LOCUS       DATLVW010000001         6773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_496215, whole genome shotgun sequence.
ACCESSION   DATLVW010000001 DATLVW010000000
VERSION     DATLVW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496215"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..856
                     /locus_tag="VNN25_00005"
     CDS             476..856
                     /locus_tag="VNN25_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89931.1"
                     /translation="MAFEKWDPQPEFERFPASATIVQSRLIRLPTPVASMLRLRGKSN
                     ANLYYDKKRKVIGIRALDKKEEGAAKIRQSDKSTSLHVPSFFRFYDIDVPGIKRFHCW
                     YDEKERMAMIDISRPSPRGRPAGT"
     gene            complement(934..2331)
                     /locus_tag="VNN25_00010"
     CDS             complement(934..2331)
                     /locus_tag="VNN25_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXH89932.1"
                     /translation="MNRIRSFARIAAVMSAVAALAVPGFAARGKADFTRFVVVGDSYG
                     AGFEAGSLNERHQVFGWPAIVAQQAGLTLCTPTSTATDPCFALPLIAYPGLPGGETVY
                     NGVGLVTVPGSGAPRMAGFARPYNDLAVPGYTVGTTMLFTGAEPTSGLGQAILRGLGT
                     EVDQAVALHPTFIAIWIGGNDFLGAVGAGSPAGLTPLATFTTQYGAMLDKLTAGAPTA
                     GMVVGTLPNNFLVVPLTSTLPTVAITPSLAPVIVNGAPIPLFTILPDGTPGPLPTGSI
                     VPLSALPLLQQGYGLPPQLKTVAPFSALPHTGEPLPDAGFISPAEQAVFVQRINDYNA
                     AIVAAAGARDIPVADITGLFNSFIPGLNFGGVALTKTFISGGIFSNDGTHLSDIGYTL
                     FANQYIKAINAGYGTHIPVASITKFFQNNGQNFGFGASANVVISPEAAQNMTVLSISA
                     PEPAPAPAPRRRAGH"
     gene            complement(2360..3646)
                     /locus_tag="VNN25_00015"
     CDS             complement(2360..3646)
                     /locus_tag="VNN25_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HXH89933.1"
                     /translation="MRSFSKIGLLVSVAVLAVSSAAFGSGFGIFEQGAKATAMGGAFV
                     ATADDPSAIFYNVAGIAQQRHTTFLAGGTLINFSNEFTGDPNDPLTVGTTGQYAKHTF
                     VPPNAYLIVPVGSNLTFGVGVFTPFGLRTNWEEPWVGRFVSRDANVKTLSVEPAIAWQ
                     TTDGRLAIGAGVEYRRSHIALNRNNAGFNPLTGRVFDSANAFLNSDWDTTTGWNVGIL
                     LKPAPTWRFGLSYRADSTIDYKGKAHFTQILTGTPLDAVVATQIPPDQTIATSINFPA
                     ILAAGIATSALPGWDLEADITHTTWSRFQTLDVTFGTTTALNFSRPQDWKDTYSYRLG
                     ANKKVNDVWDVRFGGVYDKNPQPTAGVGPLLPDSDRLGASFGIGWHSGPFILDLTEMA
                     LHFKNRSTVGVVQNDNFHGSYKTNANLIGVNAGYRF"
     gene            complement(3911..5311)
                     /gene="gatB"
                     /locus_tag="VNN25_00020"
     CDS             complement(3911..5311)
                     /gene="gatB"
                     /locus_tag="VNN25_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXH89934.1"
                     /translation="MTWEAVIGLEVHAHLSTDTKIFCACATSFGAPPNTQVCPVCLGY
                     PGALPVLNRRAVELAIRAALATGCAIHERSVFARKNYFYPDLPKGYQISQFDRPLATG
                     GSIGNVRILRIHMEEDAGKSIHHGEVSHVDLNRAGTPLVEIVSEPDIRSAEEADAYLT
                     RLRQILMYTEVCDGNMEEGSLRCDANVSVRHTGAGLGTRAEIKNLNSFRYLRSAIDFE
                     IARQIAILESGGTITQETRLYDPASGETRTMRSKEEAHDYRYFPDPDLLPLVVDRAWI
                     DEIARSVPELPHVRADRYQRDFGLSAKDAEALVTSRELADYFEATVESSGNARGAANW
                     VRNEVLRVLNEQKLDLASYRVTPGKLGRLIRLIDSGAIGGKSAKEVFDDMSATGDEPE
                     AIVERKGLSQISDPAVIREAAQGVIERNPAQVEQYRSGKQQLFGFLVGQLMKETRGKA
                     NADLANQILRELLDQA"
     gene            5389..>6773
                     /locus_tag="VNN25_00025"
     CDS             5389..>6773
                     /locus_tag="VNN25_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89935.1"
                     /translation="MKRAALLRVYLLVTISVTAMATLWTLSTSTWASSQTLLFYAALI
                     AIAAWVRVDDEVSGFEAAVAFAAILVLHDPVVALVSVFAGTAAYYVARDGRRRTFRLE
                     SLCDAAQLALSCYVVAWLYSSAVAKNAPLMAKVSGYVLLVVGYLIVHLAFVAIRQGLA
                     GNGDAIDVRRILIAQGRMLLLMSPVVAVEVLSYAAYGKLGFALAFLPVLLVAYSMRNE
                     TDAVRQNAELTRRNRELSILTESASQILTAEGDQETLRRMVALLGRLAKLKACAIVTW
                     GVNPDIPATVYRHGFCDPTDQDILAWVESAGFAESAPSRAFVFHNDQRRFPLSEGNAI
                     QVLIGIQTAEVIYGILVYETEDPSILKSGSMNLLTLLVNQAGVSLQDQLLRREMVEKT
                     TQLEAQAATKSLILEVANGLIGSFDVDAMLTRIALAIGKALGFEIVVFALFDAKREEF
                     NRRAHAGLDDV"
BASE COUNT         1225 a         1969 c         2290 g         1289 t
ORIGIN      
        1 cgaggcggtg aactaccgcg cgctggaccg cgcgtatttc aaaacggggt gaggctagtc
       61 aaaaccgatc accacagaga cacagagagc acagagtttt cgttgttacc tctgtgatct
      121 ctgtgtctct gtggtggtcg gtctcctggg gcagccgggc gcaagcattt agcctgccat
      181 ccgcgaagct cgcccgaaag gaccgaaaac gggcctgaag acggcaggtt ccagccccct
      241 cgctcgccca cgcaagttga ttggctgccg tacgttagcc gcagggagcc gcccgccttc
      301 cctgcgcggt aatgtccgcc cgatagccac tcttaatcac tcagaataat ttaatgcatc
      361 cgggtggttt tggagcgggc gaaagaagtg cgattaattg ttgacaaaat cggcttcgat
      421 tttatgatct tatcacctac gccagcaggg tacgtgatgg gtgaggagtc ggagaatggc
      481 atttgaaaag tgggatccgc agcctgaatt cgagcgcttt ccagcctcgg ctacgatcgt
      541 tcagtcgcga ttgatccgcc tgccgacgcc tgttgcatcc atgctccgct tgcgcggaaa
      601 gagcaacgcg aacctctact acgacaagaa gcgcaaggtc atcggcatcc gcgcactcga
      661 taaaaaagaa gaaggagcgg cgaagattcg tcagtccgac aagtcgacgt cgctgcacgt
      721 cccgagcttc ttccgcttct acgacatcga tgtgcccggc atcaaacgct tccattgctg
      781 gtacgacgaa aaagagcgga tggcgatgat cgacatctcg cgtccatcgc cgcgtggacg
      841 gccggcggga acgtagcgcc ccttttcgaa atcgagcaac caacaaaaag ggcggccaat
      901 cggccgccct tcgtattgaa gctgatgctg ggattagtga ccggcgcggc ggcgcggcgc
      961 gggtgccgga gccggctcag gcgcgctgat cgaaagcacc gtcatgttct gcgccgcttc
     1021 cggcgagatc acgacattcg ctgatgcacc gaatccgaag ttctgcccgt tgttctggaa
     1081 gaacttcgtg atggacgcga cagggatgtg cgtaccgtag ccggcgttaa tcgccttgat
     1141 gtactggttg gcgaagagtg tgtagccgat gtcgctcagg tgcgtgccgt cgttgctgaa
     1201 gatcccgccc gagatgaacg tcttggtgag ggcaacgccg ccgaagttga gaccagggat
     1261 gaaactgttg aagaggccag tgatgtcggc gactggaatg tcgcgcgcgc ctgcggcagc
     1321 gacgattgcg gcgttgtagt cattgatgcg ctgcacgaaa acggcctgct cggccggcga
     1381 gatgaagccg gcgtccggca gcggctcacc ggtgtggggc agcgcgctga atggtgcgac
     1441 agtcttgagt tgcggcggaa gtccgtagcc ctgctgcaga agagggagtg cgctgagcgg
     1501 aacgatggag ccggtcggaa gtggtccggg ggtaccgtcc gggaggatcg tgaacagcgg
     1561 aatcggagca ccgttgacga tgaccggcgc gaggctcggc gtgatcgcaa cagtcggcag
     1621 ggtagaggtc agcgggacga caaggaagtt gttcggaagc gtgccgacga ccatgcctgc
     1681 cgttggagcg ccggcggtga gcttatcgag catcgcgccg tactgcgtcg tgaacgtcgc
     1741 cagcggggtg aggccggccg gtgagccggc gccgacggca ccgaggaagt cattgccgcc
     1801 gatccagatg gcgatgaacg tcggatgcag ggcgacggcc tgatcgactt cggtgccgag
     1861 gccgcgaagg atggcctgtc cgagtccgga ggttggttcc gcgccggtga acaacatcgt
     1921 ggtgccgacg gtgtagcccg gcacagcgag gtcgttgtag ggacgggcga aaccggccat
     1981 gcgaggtgcg ccggagcccg ggacggtcac gaggcctacg ccgttgtaga ccgtctcgcc
     2041 gcccggcagt ccgggatagg cgatcagcgg cagcgcgaag caggggtcgg tcgcggtcga
     2101 tgtcggcgtg caaagcgtga gaccggcctg ctgggcgacg atggccggcc agccgaagac
     2161 ctgatgacgt tcgttgagag agccggcttc gaaaccggcg ccataactgt caccgacgac
     2221 aacgaagcgg gtgaagtcgg ctttgccgcg cgcggcaaag ccaggaacag cgagggccgc
     2281 gacagcagac atcaccgcgg cgatgcgggc gaagcttctg atgcgattca tatgggtttc
     2341 tcctttgtgg gttcgccgct tagaagcgat aaccggcatt gacgccgatc aggttcgcgt
     2401 ttgttttgta gctgccatgg aagttgtcgt tctgcacgac accgaccgtg ctgcggttct
     2461 tgaagtgaag ggccatttca gtgaggtcga ggatgaacgg cccgctgtgc cagccgatac
     2521 cgaacgacgc gccgaggcgg tcggagtccg gaaggagcgg tccgacgccc gcggtgggct
     2581 gcgggttctt gtcgtacacg ccgccgaagc ggacgtccca gacatcgttg actttcttgt
     2641 tggcaccgag ccggtaggag taagtgtcct tccagtcctg cggacggctg aagttgagcg
     2701 ccgtcgtcgt gccgaaggtg acgtcgagtg tctggaagcg gctccacgtg gtgtgggtga
     2761 tgtccgcttc gagatcccag ccggggagcg ccgaggtggc gatgcctgcg gccaggatcg
     2821 cggggaagtt gatggaggtg gcgatcgtct gatccggagg aatctgcgtg gccacgacgg
     2881 cgtcgagagg tgtgccggtc aggatctgcg tgaagtgagc cttgcccttg tagtcgatcg
     2941 tcgagtcggc gcgataggac agcccgaagc gccacgttgg cgccggcttg agcaggatgc
     3001 cgacgttcca gccggtggtc gtgtcccaat cgctgttcag gaatgcgttc gcggaatcga
     3061 agacgcggcc ggtcagcggg ttgaatccgg cgttgttccg gttgagtgcg atgtgcgagc
     3121 ggcggtactc gacgccggcg ccgatggcga ggcggccatc agtggtctgc cacgcgatcg
     3181 caggctcgac cgagagagtc ttcacgttgg catcgcgcga gacgaagcgg ccaacccagg
     3241 gctcttccca gttcgtgcga agtccgaacg gcgtgaagac gccgacgccg aacgtgagat
     3301 tgctgccaac cggcacgatg aggtacgcat tcggcgggac gaaggtgtgc ttcgcgtact
     3361 gccccgtcgt gccgaccgtc agcgggtcgt tcgggtcgcc ggtgaactca ttgctgaagt
     3421 tgatcaacgt ccctccggcg aggaaggtgg tgtggcgctg ctgggcgatg cctgccacgt
     3481 tgtagaagat ggcgctggga tcgtccgcgg ttgccacgaa cgcgccaccc attgctgtag
     3541 cctttgctcc ctgttcgaag atgccgaatc cggagccgaa cgcggcgctg ctgactgcca
     3601 gcaccgccac tgaaaccaat agcccgattt tgctgaaaga acgcaagctg gacctcctaa
     3661 gtaaaactcg ctaagtgact cgaattgcgc tggaaagtgg atgtctgaag aaccgctcga
     3721 tcatagcata gcgattacgg aagaaatggc agccgaattc atacgaagga tgagggatga
     3781 aaggcgtgaa gcgtagcgcc gccggcccgg cggctggacc gccggcggct cgccggcgac
     3841 cgtcgtaaga accgccaccg agccggtggc gatacagccg acgagccgtc ggcgctacgc
     3901 tactcatccg tcatgcttga tcgagcagtt ccctaaggat ctggttggcc aggtccgcgt
     3961 tcgccttccc gcgcgtttcc ttcatcagtt gaccgaccag aaagccgaag agctgctgtt
     4021 tgccggatcg gtactgctcg acttgcgcgg ggttgcgctc gatgacgccc tgcgcggcct
     4081 cgcggattac cgccgggtcg ctgatctgcg agagcccttt gcgctcgacg atcgcttccg
     4141 gctcgtcgcc ggttgccgac atgtcatcga acacttcctt cgctgatttg ccgccgatcg
     4201 ctccgctgtc gatgaggcgg atcaatcgac cgagcttgcc cggtgtgacg cggtacgacg
     4261 cgagatcgag tttttgttcg ttgagcacgc gcagcacttc attgcgcacc cagttcgcgg
     4321 cgccgcgagc gtttccgctg ctctcgacgg tcgcctcgaa gtaatccgca agttccctcg
     4381 acgtcaccag agcctcggca tccttcgcgc tcaagccgaa gtcgcgctgg tagcggtccg
     4441 cgcggacgtg cggcagttcc ggcactgatc gggcgatctc gtcgatccat gcgcgatcga
     4501 cgacgagggg caacaggtcc gggtcaggga agtagcggta gtcgtgcgcc tcttccttcg
     4561 agcgcatggt ccgcgtctcg cccgacgcag gatcgtagag ccgggtctcc tgcgtgatcg
     4621 tgccgcccga ttcgaggatc gcgatctgcc gggcgatttc gaaatcgatc gcacttcgca
     4681 ggtagcggaa cgagttcaga ttcttgatct cggcgcgcgt gccaaggccg gcgccggtat
     4741 gacgaaccga gacgttggcg tcgcagcgca gcgacccttc ctccatgtta ccgtcgcaga
     4801 cctcggtgta catcagaatc tgccgtaacc gcgtgaggta ggcatcggcc tcctcggcgg
     4861 accgaatgtc cggttccgag acaatctcga cgagcggcgt gccggcgcgg ttgagatcga
     4921 cgtgcgagac ctcgccgtgg tggatcgatt tgccggcgtc ttcctccatg tggatgcgca
     4981 ggatccggac gttgccgatg ctgccgcccg tggcgagggg ccggtcgaac tgcgagatct
     5041 ggtagccctt tggcaggtct gggtagaagt agttcttgcg ggcgaacacg gagcgctcgt
     5101 ggatcgcgca accggtggcg agcgcggcgc ggatggccag ctcgaccgca cggcggttga
     5161 gaacgggaag cgcaccaggg tatccgaggc agacgggaca gacttgcgtg ttcggcggag
     5221 cgccgaatga ggtggcgcac gcgcagaaga tcttagtgtc cgtcgaaaga tgtgcgtgca
     5281 cttcgaggcc gatgacggct tcccaggtca tgggcgcgga ttatctgtca caagtgcgat
     5341 tcagcgattg agggctggac gagtacaatt ccatcgggtt cggggtttat gaagcgtgcg
     5401 gctttgctac gcgtctacct gcttgtgacg atcagcgtca ctgcgatggc cacgctgtgg
     5461 acgctcagca cctccacttg ggcctcctcg cagacgctgt tgttttacgc ggcgttgatc
     5521 gcaatcgcgg cctgggtacg cgtcgacgat gaggtcagcg gcttcgaagc cgccgtcgcc
     5581 ttcgcggcga ttctcgttct gcacgatccg gtagtcgcgc tggtttcggt cttcgccggc
     5641 acggcggcgt attacgtagc gcgcgacggc cggcgccgca cattccggct cgagtcattg
     5701 tgcgacgcgg cgcagctcgc gctctcctgc tatgtcgtgg cgtggctcta ctcgtcggcg
     5761 gtcgcgaaga acgcaccgct gatggcgaag gtctccggtt acgttcttct cgtcgtcggc
     5821 tatctcatcg tgcacctggc gttcgtggcc atccggcaag ggctcgccgg caatggtgac
     5881 gccatcgacg tccgtcgcat cctcatcgcg caggggcgga tgctgcttct gatgtcgccc
     5941 gtcgtcgccg tcgaggtgct gtcgtacgcg gcgtacggaa agcttggctt cgcactggcg
     6001 ttcctgcccg tgctgctcgt tgcatactcg atgcgaaacg agacggacgc ggtgcggcag
     6061 aacgcagaac tgacgcggcg caaccgcgag ctctcgatcc tgaccgaaag cgcgtcgcag
     6121 atcctgactg ccgaggggga ccaggaaacg ttgcgacgca tggtcgccct cctcgggcgt
     6181 ctcgcgaagc tcaaggcatg cgcgatcgtc acttggggcg tgaaccccga catcccggca
     6241 acggtctacc gccatggatt ctgcgacccg accgatcagg acatcctcgc gtgggtcgaa
     6301 tcggccggct tcgcggagtc ggcgccgagc cgagcgtttg tgtttcacaa cgatcagcgc
     6361 cggttcccgc tgtcggaagg aaacgccatc caggtgctca tcggcattca gacggccgag
     6421 gtcatttacg ggattcttgt gtacgaaacg gaggacccgt cgatcctcaa gagcggcagc
     6481 atgaacctgc tgacgttgct cgtgaatcag gccggcgtct cgctgcagga ccagctcctg
     6541 cggcgcgaga tggtggagaa gacgacgcag cttgaggcgc aggcggcgac gaagtcgctg
     6601 atcctcgagg ttgcgaacgg actcatcggc tcattcgacg ttgacgccat gctcacgcgc
     6661 atcgcgttgg cgatcggcaa agcgctcggg ttcgagatcg tcgtcttcgc gctgttcgac
     6721 gccaagcgcg aggagttcaa ccgccgcgcg cacgcgggac tcgatgatgt atg
//
LOCUS       DATLVW010000002         4506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_372228, whole genome shotgun sequence.
ACCESSION   DATLVW010000002 DATLVW010000000
VERSION     DATLVW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372228"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VNN25_00030"
     CDS             complement(<1..890)
                     /locus_tag="VNN25_00030"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXH89936.1"
                     /translation="MAENRQFRYAHDVNTSAFSRELSARVEAYFAERGIWRHANLEMY
                     SKTVLGLVMTVAAYYWLMTGHFTALEVIGVYVILGTAQLYSAFNIAHDGNHSAYSDKK
                     WVNRVAGCVFDAIGVSSYMWRLLHNTAHHSFVNIRGADTTLVSGNIFRFSPHDQRRPL
                     HRFQHLYAPFFYCLSSLDWVLTKDFRWLMPGKSFGNRKQIKHPLSELIVLFAFKAFYY
                     TYMLVLPLLLLDVPWWSILIGFVALHACMGFAIAVIFQPNHFNEDATYPEADEEGHIG
                     NNYIQHVFDTTSDYANHPVK"
     gene            complement(1019..1690)
                     /locus_tag="VNN25_00035"
     CDS             complement(1019..1690)
                     /locus_tag="VNN25_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89937.1"
                     /translation="MTERHQSIFYAAGYAHALDKQVILLTKDEEDIPFDLKHYPHIVH
                     GGRLTDLRAELERRACHLLDAAARGDAPLATPVEISVNDVALSPDVPRLVKANDQGRS
                     GLWFDIEIRNQGGWRLRPASMQLGLITPGHYFEASADGPLYVENIPIDAGRRLFLSQE
                     EHTILPGSWETTGFIAHTRQWSDARESEPFAIRVLTESGYFDFPFTVQWPVTSEPTSA
                     ESGGA"
     gene            complement(1884..2765)
                     /locus_tag="VNN25_00040"
     CDS             complement(1884..2765)
                     /locus_tag="VNN25_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89938.1"
                     /translation="MPPRRTSSTAPRPFAFVLMPFAAAFDDVYEIAIKPACEAAGAYA
                     ERVDDQIFQGNILQRVYHQIAKADLIVGDLTGRNVNVLYETGYAHALRKHVLLLTRGS
                     GDVVFDPAHHPYIVYERLVDLRTELERKVRNLLETMEQRTSPVAIPVSVMIDDVLLDP
                     IVARDVVVRQRDDKGWIGFHVVVQNPALRVIRPLALQIGMIAPTHLEVTTRFEGVRMD
                     STRLGADAVLRILRMDFTLLPGAGTKLWMTCYPKRKYEEGEAVGAFAVRVMTVEGYYD
                     FAFNLTAAPPEPEKSPE"
     gene            complement(2868..4040)
                     /locus_tag="VNN25_00045"
     CDS             complement(2868..4040)
                     /locus_tag="VNN25_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXH89939.1"
                     /translation="MTNGNQSAKLYEEGPGGKAVGWRGVIPGAILLVGLSVLCLYALV
                     ALWPHPTPSGTLKDSNAPTTATSTAPTTTAATATGTSPATNTATSSSAANTTAAPSTS
                     TSTAPPSAANNIPSLSASTAATQQRAKECADVNDKDECDCLARVDYKRKLHDQEKSPD
                     SPRLKDDPTCVYIFGGWHLIWNETRLILVVILSGFLGALVYSLRSFFWYTGNRELKWS
                     WLLMYLLVPIVGAMLAVIFYMLLRGGLFSPTTTVADTSPFGFAAIAALVGMFTNQASE
                     KLRTVFETLMTKPATGRDASTVVPPTITSAAPSSVIAGVDTVLTLKGSGFTQTSVVII
                     GNEELKPTVLSANELTVGIPAALSAKATVLKLVVSTPGAGKSSEVNVNVSTAPPPQ"
     gene            complement(4249..>4506)
                     /locus_tag="VNN25_00050"
     CDS             complement(4249..>4506)
                     /locus_tag="VNN25_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HXH89940.1"
                     /translation="ASRRVSAGSPDYWDYATLLELAVLGKNEAEAVENLTRALPIIRA
                     KWEPETTLNNLKMIRKARKDRNEEEPAWIEDVMNALDAAAQ"
BASE COUNT          871 a         1333 c         1383 g          919 t
ORIGIN      
        1 ttcttgacgg gatgattcgc gtaatccgag gtcgtgtcga agacatgctg gatgtagttg
       61 ttgccgatgt gtccctcttc atccgcctcc ggatacgtcg catcttcgtt gaagtgattg
      121 ggctggaaga tcaccgcgat cgcgaagccc atgcaggcgt gcaacgcaac gaacccgatg
      181 aggatcgacc accacggcac atcgagcagc agcaacggca gcacaagcat gtacgtgtag
      241 tagaaggcct taaacgcgaa cagtacgatc agctcactca acggatgctt gatctgtttg
      301 cgattgccga aactcttgcc cggcatcagc cagcggaagt ctttcgtcag cacccagtcc
      361 aatgacgaca agcaatagaa gaacggtgcg tagagatgct ggaagcgatg gagcggccgg
      421 cgctggtcgt gtggcgagaa gcggaagatg ttgcctgaca caagcgtggt atcggcgcca
      481 cggatgttta cgaaagagtg atgcgcggtg ttgtgcagca ggcgccacat gtaagagctc
      541 acaccgatcg cgtcgaacac gcagccagcc acccgattca cccatttctt gtcggagtag
      601 gcgctgtgat tcccgtcatg cgcgatgtta aaggcgctgt acaactgcgc ggtaccgagg
      661 atcacgtaga caccgatgac ttccagcgcg gtgaaatggc cagtcatcaa ccagtagtac
      721 gcggcgacgg tcatcaccag gcccaggaca gtcttggagt acatctccag gttcgcatga
      781 cgccagatac cgcgctccgc gaaataagcc tccacgcgcg cagagagctc tctgctgaac
      841 gcggatgtat tcacgtcgtg cgcgtacctg aactgacggt tctcggccat gcggacacct
      901 atttagactg atggagtgaa ggacggagct tacaccaacg ctgcggcgga aaccagggag
      961 tggagaccgg caattggtga cacgcgtcgg tgctagccgc ttacttagga cgaagcgatc
     1021 acgccccgcc agattctgcg gatgttggtt cgctcgtgac cggccactga acggtgaacg
     1081 ggaaatcaaa gtatccgctc tcagtgagga ccctgatcgc gaacggttcg ctttcgcgcg
     1141 catcgctcca ttgacgcgtg tgcgcgataa aacccgttgt ctcccatgag cctggaagga
     1201 ttgtgtgctc ctcctgtgaa aggaaaaggc gtctgccggc gtcgatcggg atgttctcga
     1261 cgtaaagggg gccgtcagcg gacgcctcga agtaatgacc cggcgtgatc agcccgagct
     1321 gcatgctcgc aggtctcagc cgccatcctc cctgatttcg aatttcgatg tcgaaccaca
     1381 gaccgcttct tccctggtcg tttgctttca ccagtctcgg gacgtcgggc gagagtgcta
     1441 catcgttcac gctgatctcg accggcgtcg cgaggggagc gtcgccccgc gccgcagcat
     1501 cgagaagatg acaggcacgc cgctcgagct cagcccgcag gtcggtgagg cggcctccat
     1561 ggacaatgtg tgggtagtgc ttgaggtcga acggaatgtc ctcttcgtcc tttgtcagca
     1621 gaatcacctg cttgtcgaga gcatgcgcat agccggctgc gtagaaaata ctctggtgcc
     1681 gttccgtcat gtcggcgatg acgaggtcgg cttttgcgat caggttctgg atacgctgaa
     1741 gggtcgtttc cggaaaaagt tgggcgtcga gccgctcggc atacgcaccg ctgcttcaca
     1801 ggcaggattg atggcgagtt cccaggaatc gtcgaacgct gcgcctgacg gaacgagggc
     1861 gagagcaaat ggccgagggc cgctcattcg ggactcttct ccggctcggg cggcgcggcc
     1921 gtgaggttga atgcgaagtc gtagtatccc tcgacggtca tcacacgaac ggcgaacgct
     1981 ccgactgctt caccctcttc gtatttcctc ttgggatagc acgtcatcca gagcttcgtc
     2041 ccagctccag gaagaagcgt aaagtccatt cgcaggatcc gaaggactgc gtcggcgccg
     2101 agccgcgtcg aatccatcct caccccctcg aagcgagtgg tcacttccag gtgagttggc
     2161 gcaatcattc caatctgcag agcgagcggc cggatgacac gaagggccgg attctgcacc
     2221 acgacgtgaa aaccgatcca tcctttatca tctcgctgcc tgacgacgac gtcccgtgcg
     2281 acgatcgggt cgagtaagac gtcgtcgatc atgaccgaaa ccggaatcgc gaccggcgat
     2341 gtccgctgct ccatcgtctc gaggagatta cgtactttcc gttcgagctc cgtgcgaaga
     2401 tcgaccagcc tttcgtacac gatatacgga tggtgtgccg ggtcgaacac gacatcgccg
     2461 ctaccgcgcg tcagaagaag cacgtgtttg cgcagagcat gcgcataccc cgtttcataa
     2521 aggacgttga cgttccgtcc ggtcaagtct ccgacgatga gatcggcctt tgcgatctgg
     2581 tgatacacac gctgcagaat gttgccctga aaaatctgat cgtcgactcg ttcggcgtac
     2641 gcccccgccg cttcgcacgc cggcttgatc gcaatttcat atacgtcatc gaatgccgcg
     2701 gcaaagggca tgagcacgaa ggcaaacggc ctgggcgcgg tgctggaagt gcggcgcggc
     2761 ggcattccgc aacagtttat tcgataagca cttcgccgcc atccgccatc tgcggcgatt
     2821 ctccggcggc tcagaggatg gccaccaaga cgatgcgcgc ggcctcctta ctgcggcggc
     2881 ggcgccgtcg agacattcac attgacttca ctcgacttcc cggcgcctgg cgtcgaaacg
     2941 acgagcttga ggacggtcgc tttcgcgctg agcgcggcgg ggattccaac tgtgagctcg
     3001 ttcgcggaga ggaccgttgg cttcaactcc tcgttgccga taatgaccac cgacgtctgc
     3061 gtgaagccgc ttcctttgag ggtcaacacg gtgtcgacgc ccgcaatcac acttgatggc
     3121 gcggccgacg taatcgtcgg cggaacgacg gtggaggcgt cgcggcccgt cgccggcttc
     3181 gtcatcagcg tctcgaaaac ggttcgcagt ttttccgacg cctgattcgt gaacatgccg
     3241 acgagcgcgg cgatcgcggc gaagccgaac ggactggtgt ccgcgacggt tgtcgtcggc
     3301 gagaagagtc cgccgcgcaa gagcatgtag aagatcactg cgagcatcgc gccgacgatc
     3361 ggcacgagca ggtacatcag cagccagctc catttgagct cgcgatttcc cgtgtaccag
     3421 aagaaggagc gaagcgaata caccagcgct cccagaaatc cggagaggat gacaacgagg
     3481 atgaggcgcg tctcgttcca gatgagatgc cagccgccga agatgtacac gcacgtcgga
     3541 tcgtccttga ggcgcgggct gtcgggcgac ttctcctggt cgtgcagctt gcgcttgtag
     3601 tcgacccgcg ccagacagtc gcactcgtcc ttgtcgttga cgtcggcgca ctctttcgcg
     3661 cgctgttgcg tcgcagccgt gctggcggat agtgacggta tgttgttcgc ggccgagggc
     3721 ggtgccgtgc ttgtcgatgt tgacggcgcg gctgtcgtat tcgcggcgga tgatgacgtg
     3781 gccgtgttcg tcgccggcga ggtccctgtc gcggtcgcgg cggtagtggt cggcgcggtg
     3841 ctcgtcgcgg tggtcggcgc gttcgaatcc ttcagcgttc ccgatggcgt cggatgcggc
     3901 cacaacgcca cgagcgcata gaggcagagc actgagagtc cgacgagcag aatcgcgccg
     3961 gggatgactc cgcgccatcc cactgctttt ccgcctggac cttcttcgta cagtttcgcg
     4021 gattgattgc cgttcgtcat agcgactcgt ctccgacggc gcgatgccgc aagatctctg
     4081 tctagtttga gagggtgaac gcacgaatta tacgcgctcg gtatcaggct ttgatcaacg
     4141 ttcccgcggc gggcaggcca aattgtctct ggcggcggtt cggcgctccg tgctgcgcgc
     4201 gacgtgcccg aatcgtctca tgacgtcgaa cgtcagccgt cgactctgct attgagccgc
     4261 ggcgtcgagc gcgttcatca cgtcctcgat ccacgccggc tcttcttcgt ttcgatcctt
     4321 tcgcgccttg cggatcatct ttaggttatt gagcgtcgtc tcgggctccc atttcgcccg
     4381 aatgattggt agcgcgcgcg tgaggttctc cactgcctcg gcctcgttct ttcccaggac
     4441 ggccagctcg agcagcgtcg cataatccca gtagtccggg cttcccgcgg agacgcggcg
     4501 cgacgc
//
LOCUS       DATLVW010000003        10290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_578982, whole genome shotgun sequence.
ACCESSION   DATLVW010000003 DATLVW010000000
VERSION     DATLVW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578982"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VNN25_00055"
     CDS             complement(<1..287)
                     /locus_tag="VNN25_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89941.1"
                     /translation="MSNENEVKQNNGAAAADGQSVTPEAIVETLRALREQIPEYVQLA
                     PADAKSIQPVASVHPDFAQAAINAIGASPMVQAVVGQTPEALQQDAESAAR"
     gene            1301..2701
                     /locus_tag="VNN25_00060"
     CDS             1301..2701
                     /locus_tag="VNN25_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HXH89942.1"
                     /translation="MALPKVSLTQKIFIGLAIGVLVGWLWPDWGTAMRPLSLLFLNLI
                     KSIIAPLIFATLVIGIAGTGDIRQVGRIGFKALLYFEIVTTFALFIGLAAVNLTKPGV
                     GVSLAGVHTEVNKAVLADRASKLAKEANLAAAHGDNVTAAAKASQAADAVGQGLTAPD
                     PPAKPQKFGEIIAHLAPASILKAMAEGDVLQIVVFSVLFALAVTSIGEKARPVITWCE
                     SLADIMFRFTEFVMKFAPIGVGAAMANTIGHSGLGVLKNLGMLVLTLYGALVVFLLCV
                     LLPVALLFRVPLRDFFRAVKVPATIAFATTSSESALPKAMENMERLGVPRRIVGFVLP
                     TGYSFNLDGTTLYLALASIFVAQAAGVHLSWTQQIIMLLTLMLTSKGVAGVPRASLVI
                     LLGTLASFNLPAEGVIVILGVDELMDMARTCVNVIGNCLATVVVARWEGAFRTNEWIA
                     EEAELEQVTGLPAIAG"
     gene            2748..3191
                     /locus_tag="VNN25_00065"
     CDS             2748..3191
                     /locus_tag="VNN25_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89943.1"
                     /translation="MKRTYAFLAAVLLLAGCASSAREDSNASKITLHLEQVDTRVNIF
                     ASPGPVTVQFVLTGTNTTTEPLTLARLEVRSISDGAYTIAPVTRTLNVQLAPGQSQQL
                     PISLWGNAHGGRGYSAAPVILRGTGYMTSPKGHFAKLFTETIAQR"
     gene            3350..3796
                     /locus_tag="VNN25_00070"
     CDS             3350..3796
                     /locus_tag="VNN25_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89944.1"
                     /translation="MAKRIYALLAVLLLAAGCASSADDDPNAPKVTLHLEQVDTGVNT
                     FTFNGPVTVQFVLTAENTTSDPVTLTRIEIRTISTGAYSIAPVTTNVNLKLAPGQTQQ
                     SMISVWGYSRGGRLYSEEPVTVRGTGYLTGPKGAFVKLFTEYIAQR"
     gene            complement(4167..5168)
                     /locus_tag="VNN25_00075"
     CDS             complement(4167..5168)
                     /locus_tag="VNN25_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89945.1"
                     /translation="MKSIEKDSLYQVFMERYRVVLLATTLSAVVGLVLMATGEWLHDR
                     SHLWSSIATEAGKVVIVTSVVGLIFEFVMHDRFVKRVTRDVKRLDESIEVLQRTVAIT
                     SGAIESGLSAVYSDRQKAIDDIDGLLKEAKRGDHLMIAGISLGDFLCPHGRLYGRTSD
                     ALTAGINISALLLDMGSEAAKTRAQREEIGPETPKFGTPLWLEWYYETSCFDELKTAS
                     DLARSYLVRFDGQKRNAKNEQVGRFEARIYTLTPLCFLAIYGKTMFLESYHYAGRGGE
                     SPIMKIAQTTKGSQDNSRLFSIYMKHFNILCGLATPLEPPAYDPPAQTEPSGGAVAG"
     gene            complement(5485..5898)
                     /locus_tag="VNN25_00080"
     CDS             complement(5485..5898)
                     /locus_tag="VNN25_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89946.1"
                     /translation="MAFDALIYFVEKNGHKVDIASTGEQIEIALVDMHRYDLLILDII
                     MEPLPSSEYNEQYGGIDVLEKIAATGRGLPIIMLSVMPARLIRNEAIRRGLDLERIGV
                     KEIRRKGSVTPTELAASVKMHLRRRRDDLAGGAEC"
     gene            complement(5952..7637)
                     /locus_tag="VNN25_00085"
     CDS             complement(5952..7637)
                     /locus_tag="VNN25_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXH89947.1"
                     /translation="MADSIGPYSGIGWRRRFRLLDSRDLHALAYSFSDYRNSADYRNL
                     AEKYRLGSDQRSFLNSLKFGLLIPLTCSDINLGYVTLSWTEGTPNLAIAAELRSYVES
                     IQATVPLLYSACRSIITDNNLVAFWAAAGTIVSSQSEALCYENIALACLQLWPDSTTY
                     VGRIDPNASGAVIVAVEGRWKEEADQERQRERQIIPFGEGQFGYALTRNEPVISFSLA
                     DDNRFTYHSLRENNEYLGSGIAMKLSGLNGEPIAVMSVEHDIKNYFDPEDLRYITGIA
                     SLGYEALTVHKETAAILSRDIDTLFTQMLHDVTEPLQAIIADADVLRYEASIEPPKLA
                     DDELVKRLDNISVRAANIVEGSLNLYGQVRNLIDAGIEGASTRVTAGRVNLFRLLNSL
                     VDTWGDRAALQGVEIRSLFDSLRGIEVQCDETELLSALGHLIGNAIKYSFWGRRHGDQ
                     SGATKYGRYVSIVGRITLSNVLIEIQNYGIGILPSELRRVKEKFYRGHLAIKEGRAGT
                     GRGRWSAEAFFSSIGGRVDVASLYKSSDPTTTNGPYSTTVTAVMPYITSRRNS"
     gene            8027..8632
                     /locus_tag="VNN25_00090"
     CDS             8027..8632
                     /locus_tag="VNN25_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH89948.1"
                     /translation="MAFASEHPARPQLGRTMSFEEWAAMPEDEPGELVDGRIEEEEAP
                     DLVHALVVSWLVRILGTWLGRGGFVFGSEAKYALRADRGRKPDVAVYLGSDGRHLPRR
                     GIVRVPPDIVVEVVSPSPRDERRDRIEKMDEYAAFGLRFYWILDPSLQSLEVFELLGV
                     RYARAARATEGRMDPVPGCRELVIELDDLWAELSRLGTPDE"
     gene            8703..9446
                     /locus_tag="VNN25_00095"
     CDS             8703..9446
                     /locus_tag="VNN25_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89949.1"
                     /translation="MANTSVTVRVQAYGGKFVGDKMKPTLTVNIIGGGTPVLNQPIPS
                     PSTGTVAQPFQQCAARDTIVVQPPSQKAGEWWVIPDPAPGQLTVSFDLEVPSLVEFTA
                     TAGPQSVTGMASMFLIPGLQLTGVDPGLVITLHGLAVFTPTWTPATPGSVAISAEVTM
                     MCGCPITPLPPPAGVEAYWPDNEFLVFAQYWGDDGIAGQAGMTCGANSTYAGTITGLT
                     PGNTYNFFVVALQQAETNVGASSTITFTA"
     gene            complement(9495..>10290)
                     /locus_tag="VNN25_00100"
     CDS             complement(9495..>10290)
                     /locus_tag="VNN25_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HXH89950.1"
                     /translation="AASADVSIVKTLTTAGPFITGQSISYTIQVSNAGPSAATNIQVT
                     DTPTNLTITSVSGAGCPALPCTIASLASGANVAINVTAAINGAGSFNNTATATPSELD
                     PNTTNNTDTQGGVATASADVSIVKTLTTAGPFVTGQSISYTIQVSNEGPLAATNIQVT
                     DTPTNLTITSVSGAGCPALPCTIATLAAGANVAINVTATINAAGAFDNGATVNATEFD
                     PTLTNNADSSGNGGIATGALIPALGDWTLILLATMLAALGALLMRR"
BASE COUNT         1907 a         3198 c         3034 g         2151 t
ORIGIN      
        1 caacgggcgg ccgactcagc atcctgttgc aatgcctccg gcgtttggcc gacgaccgcc
       61 tgaaccatcg gcgatgctcc gatggcgttg atggccgcct gcgcaaaatc cgggtggaca
      121 ctggcgacag gctgaatcga cttcgcgtcc gcgggcgcca actgcacgta ctcggggatc
      181 tgctcgcgca gcgcgcgaag cgtctccacg atcgcctccg gcgtgacgga ctgaccgtct
      241 gccgccgcag cgccgttgtt ttgcttgacc tcattttcat tcgacatggt gaaactcctt
      301 tcctgccccg aagggcataa atttgttatg ggacggcgcg cgggctgagt gcgcagctcg
      361 gacgcagttc cgggttggaa cgtcatgagc gaaacgccgc aaaccgttcg ctcgcctgcc
      421 cccgtagtcc gcggccgcgg gggatacttc cgaggctgga ggagctaccg ctcaacccca
      481 cttgcatccg ggtccgtcgc gccatggctc aagcttgacg ggctcagtac ctcgtttgag
      541 tagggcaaca ggcagccgcg ctttgcgagt gactttgcct tcctcgcata cttagacgag
      601 gcaccccccg ctaaaaaagg ggtcaatttg ttaaaagatc gctacagcaa tcatcttctg
      661 taagaatgtc tttcgatatt ggagacgctc gtttcgaatt ggaggcccct ccgtttgcta
      721 tcggagacgc tccgtttgac attaacggcg ccccgtttga cattagccgc gcgccgttcg
      781 acattagcgg cgtcccgttt gacattagcg gcgttccgtt tgacattagc cgcgcgccgt
      841 ttgacattag cggcgtcccg tttgacatta gccgcgcgcc gtttgacatt agcggcgttc
      901 cgtttcacat tagccgcgcg ccgtttaaca ttagcgccgc tccgtttgac attagccgcg
      961 ctccattcga cattggcggc gctccgtttg acatcgaaga agctcccttc gataacgatg
     1021 ccgtgtcgat atcgaaggag agatcctcaa tgtccagcga ctcgtcgtcg ataccgaggg
     1081 gagcgcctcc gttatcgagc ctgtcgttgc cgataccgaa cagccgggcg cgaagtgagg
     1141 cgcagtaagt ccaggctggc gcactccgtc gaacacttcc tgggactcca gccaggatga
     1201 cgctgggccg acaccggctg acaccgaggg acgccagacc gcgatcagcg caccattcct
     1261 cgttgtactc acgccctccg tcccctacac ttcccgccac atggcactcc cgaaagtttc
     1321 cctcacgcag aagatcttca tcggtctggc catcggtgtg ctcgtcggct ggctctggcc
     1381 cgattggggc acggcgatgc ggcccctttc gctgctcttc ctgaacctga tcaagtcgat
     1441 catcgccccg ctgatcttcg ccaccctcgt catcggcatc gccggcaccg gcgacatccg
     1501 ccaggtgggg cgcatcggtt tcaaggccct gctctacttc gagatcgtga cgaccttcgc
     1561 gctcttcatc ggacttgccg ccgtgaatct caccaagccc ggcgtcggcg tttcgctcgc
     1621 cggcgtccac accgaagtga acaaggccgt gctcgccgac cgcgcatcga agctggcgaa
     1681 agaagccaat ctcgccgccg cacatggcga caacgtcacg gctgctgcga aagcctcgca
     1741 agctgccgat gccgtcgggc agggcctcac cgcgcctgac ccacccgcca agccgcagaa
     1801 attcggcgag atcatcgccc acctcgcgcc ggcctcgatc ctcaaagcga tggccgaggg
     1861 cgatgtgctg caaatcgtcg tcttctcggt gctctttgcg ctcgcggtga cgtcgatcgg
     1921 cgagaaggcg cgtcccgtca tcacctggtg cgagtcgctg gcggacatca tgttccgctt
     1981 caccgagttc gtgatgaagt tcgcgccgat cggagttggc gcggccatgg ccaacacgat
     2041 cggccattcc ggcctgggcg tcctcaagaa cctcggcatg ctcgtgctga cgttgtacgg
     2101 cgcgctggtc gtctttctgc tgtgcgtgct gctgccggtc gcgctcctct tccgtgttcc
     2161 attgcgcgac tttttccgcg ccgtcaaagt gcccgccacc atcgccttcg ccacgacctc
     2221 ctcggaatcc gctcttccga aggcaatgga gaacatggag cgcctcggcg tcccccgccg
     2281 catcgtcggc ttcgttctcc ccaccggcta cagtttcaac ctcgacggca ccacgctcta
     2341 tctcgccctg gcctcgatct tcgtcgccca ggccgccggc gtccatctct cgtggacgca
     2401 gcagatcatc atgctgctga cgctcatgct gacgtcgaag ggggtcgccg gcgtgccgcg
     2461 cgcctcgctg gtcattcttc tcggcacgct ggcgtcgttc aaccttcccg ccgagggcgt
     2521 catcgtcatc ctgggcgtgg acgagctgat ggacatggcc cgcacctgcg tcaacgtcat
     2581 cggcaactgc ctcgcaacgg tcgtggtcgc acgatgggaa ggcgccttcc gaacgaatga
     2641 gtggattgct gaggaggcag agttggaaca ggtgaccggc ctgccagcga ttgcgggttg
     2701 attccgttcc gactcccgac ccccgatcag cgacccccga gatgatcatg aaacgaacct
     2761 acgcattcct tgccgccgtt ctcctacttg ccggatgcgc ctcaagcgca cgcgaagatt
     2821 ccaacgcgtc gaagatcacg ctgcacctcg agcaggtcga cacgcgcgtg aatattttcg
     2881 cgagccccgg accggtcact gtgcagttcg tcctgaccgg gacgaatacg acaacggagc
     2941 cgctgacgct cgcacgcctc gaggtccgct cgatcagcga tggcgcctac accattgcgc
     3001 cggtcacgag gaccctcaac gtgcagctcg ctcccggcca gtcgcagcag ctcccgatct
     3061 cgctctgggg caacgctcac ggcggccgcg ggtactccgc tgccccggtc attctgcgcg
     3121 gcacgggcta tatgaccagt ccgaaaggcc acttcgctaa actcttcacc gagaccatcg
     3181 cccaacgctg acgcacgcca aacccctgac gtaccgtccc tcccaaccgc atcgggcgta
     3241 gctcacctgg acgctgccgg cgcgcggcat cccgcagatt cggaggaatg atgtccgcag
     3301 caggggagtt gcagccggtg accggggtgc ccggggtttg gatctgacga tggcaaaacg
     3361 aatctatgcg ttactggctg tgctgctcct cgccgcaggc tgtgcgtcga gcgcggatga
     3421 cgacccgaac gcgccgaagg tcacgttgca cctcgagcag gtcgatacgg gcgtgaacac
     3481 cttcaccttc aacggaccgg taaccgtgca gttcgtgctg accgccgaaa acacgaccag
     3541 cgatcccgta acgctcaccc gcatcgagat ccgcaccatc agcaccggcg cctacagcat
     3601 cgcgccggtc acaacgaacg tgaacctgaa gctcgccccc ggccagacgc aacaatcaat
     3661 gatctccgtc tggggctact cccgaggcgg ccgcctctac tccgaggagc ccgtcaccgt
     3721 ccgcggaacc ggctacctga ccggcccaaa gggcgcgttc gtcaaactct tcacggagta
     3781 catcgcgcaa aggtgagggg ctccaaaccc cggatgtacg atcgcgttcc caaccacgag
     3841 cgggcgtaga ctcagttttg cggaccgcag agcagacgga tcagactcta gtcgcgcatg
     3901 agctcgacgc gaggagttcg gtatccagcg gctccccggc agcttctggc gatcgggatc
     3961 acaatcttta tgacgtgaga aggactgcaa ggtcgtcgcc gcggctttat cgatatgctc
     4021 tgccctctca cgccactcct cccgtcctcg tcctgcggtg atcagatacg ttcagagttt
     4081 gcagtccaat ctgttgagcg gcctgcccac gtcctcgcag acgtctgagt cacgcatgtt
     4141 gttccggccg cacggttacg ttagagttag ccggcgaccg cacctccgga cggttcagtt
     4201 tgtgctggcg ggtcatacgc cggtggctct agcggcgtgg cgaggccgca gagaatgttg
     4261 aagtgtttca tgtaaatcga aaacaggcga ctattgtcct gggagccctt cgtcgtctgc
     4321 gcaatcttca taatgggaga ttccccgccg cgaccggcat agtgatatga ctcaaggaac
     4381 atggttttgc cgtagatcgc aaggaagcac aacggcgtga gtgtatagat gcgagcctca
     4441 aaccggccaa cttgttcatt ctttgcattt cgcttctgac cgtcgaaacg cacaaggtag
     4501 ctgcgtgcta ggtcgcttgc cgtcttcagt tcatcgaagc aacttgtttc gtaataccac
     4561 tctagccaca acggcgtccc aaactttggt gtttcaggtc caatttcctc acgctgggcc
     4621 ctcgttttcg ccgcttcaga tcccatatcg agaagcaggg cagaaatgtt gatgcctgcg
     4681 gtcaacgcat cggaggtgcg accatacagg cgtccgtgag ggcagagaaa atcaccaagg
     4741 gaaatacctg cgatcataag gtgatcgccg cgtttcgcct ctttcagaag cccgtcgatg
     4801 tcgtcgattg ctttttgacg atcgctatac accgcgctaa ggccgctctc gatggcaccc
     4861 gatgtgatcg cgacggtgcg ctgtagaacc tctatacttt catcgagccg cttcacgtcg
     4921 cgcgtgaccc gcttgacaaa acggtcatgc atgacaaact cgaagatgag gccgacgacg
     4981 ctagtcacaa taacaacttt gcctgcttcc gttgcaatgc tcgaccataa gtgtgatcgg
     5041 tcatgaagcc attcgcccgt tgccataagc acgagcccta ccactgcgga aagcgtggtc
     5101 gcaagtagga ccacgcggta tcgctccatg aagacctgat atagcgagtc cttttcaatt
     5161 gacttcatct tcttgcgctc cggctctcaa tctacgacga tctgcatcaa tccgaggcgt
     5221 gccttgaccc tgaaaactct aatccaacgt cccactatcg gcagtagctc tcgacttctg
     5281 aaagtcagca ggtcggggtg cagctggccc gactatgcca ggttgtgatg ttttaagtcc
     5341 gagtagtggc gcaacgtttt cggagccgcg ttctcggatc tagtactcaa tggggtctcc
     5401 tagtggccac gtcgatcggc aggcgatcac gcctgcatgg gccgtcagtt gaacacccga
     5461 tgacgccgca gaaagcctgt tgcattagca ttcagctcct ccggcaagat catcgcgccg
     5521 gcgcctgagg tgcatcttga cgctggcggc caactcggtc ggcgtgaccg accccttgcg
     5581 tctgatttcc ttcacgccaa ttcgctccaa atctaatcct cggcgaattg cctcgttcct
     5641 gatgagacgt gcaggcatga cgctgagcat gataattggg aggcctcggc cggtcgctgc
     5701 gatcttctcc agtacatcga ttcccccata ttgttcgttg tactcggacg atggcagggg
     5761 ctccatgatg atgtccagaa tcaggagatc gtagcggtgc atatcgacaa gagcaatttc
     5821 gatctgctcg ccggttgacg cgatatcgac tttgtggcca ttcttctcta cgaaatagat
     5881 caaagcgtcg aatgccattc gcctcggggt acccttcccg gcgttgtcgt caatccagag
     5941 cagtctagcc atcacgaatt cctcctggaa gtgatgtatg gcatcactgc cgtgacagtg
     6001 gtcgaataag gaccgttcgt ggtcgttgga tccgagctct tgtagaggga ggcgacatcc
     6061 acccgacctc ctatgctgct gaagaaggct tcggcgctcc agcgccctcg gcctgtaccg
     6121 gccctgccct ctttgatggc gaggtgtccg cgatagaact tctctttcac tcttcggagc
     6181 tcactcggaa ggattccgat tccataattc tgaatctcga tcaatacatt gctcaacgta
     6241 atacggccca caatgctcac gtaacgacca tacttcgtgg cgccgctctg atcaccgtgg
     6301 cggcgtcccc aaaatgagta tttgatggcg ttgccaatca aatgccccag tgcggacaag
     6361 agctcggttt cgtcgcattg gacttcgatg ccccgcagcg aatcgaacag cgatcgaatt
     6421 tcaacccctt gcagcgccgc cctgtcgccc cacgtatcca caagtgagtt gagcagcctg
     6481 aagagattga cccgccctgc cgtaactcgc gtcgacgcac cttcaatgcc tgcgtcgata
     6541 aggttacgta cctgcccata gaggttcaaa gatccttcga cgatattcgc cgcccgcacc
     6601 gatatattgt cgagacgttt caccagttca tcgtccgcta acttgggagg ctcgatactc
     6661 gcctcgtatc gcaaaacatc ggcatcggcg atgatggcct gcagtggctc cgtgacatcg
     6721 tgcagcatct gcgtgaacag cgtatcgatg tctctcgata agatggcggc ggtttccttg
     6781 tgaacggtca aagcctcgta gcccagcgag gcgatgccgg tgatgtacct aagatcttcc
     6841 gggtcgaaat agttcttgat gtcatgctcg accgacatca ccgcgatcgg ttcgccgttc
     6901 aggccgctga gcttcatcgc gattccggat ccgaggtatt cgttgttctc tctcaacgag
     6961 tgataggtga atcggttgtc gtcggcaagc gagaacgaga tgacaggctc gttccgggtg
     7021 agggcgtagc cgaactggcc ttcgccgaag ggtatgatct gcctttcgcg ctgccgctcc
     7081 tggtccgcct cctccttcca tctcccttcg acggcgacga taaccgctcc agaggcattg
     7141 ggatcaattc tgccgacata ggtggtggag tccggccaaa gttgcaggca cgccagtgcg
     7201 atgttttcgt aacaaagcgc ctccgactgg gatgacacga tcgtgccggc ggcagcccag
     7261 aacgcaacta ggttgttgtc ggtaatgatg cttcggcagg ctgagtacag gagaggcacc
     7321 gttgcttgaa ttgactcaac gtaggaccgc agctcagcgg cgatcgccag attgggcgtg
     7381 ccctcggtcc acgatagcgt gacgtacccg aggttgatat ctgagcatgt gagcggaatc
     7441 agaaggccaa atttcaaaga gttgagaaag gatcgctggt cgctgcctaa gcggtacttc
     7501 tctgccagat tgcgataatc agcactgttg cgatagtcgg agaatgagta tgcaagcgcg
     7561 tggagatccc ttgaatcaag caagcgaaag cggcggcgcc aaccgattcc cgagtatggg
     7621 ccgatgctgt cggccattgt gtagaacttg ccgaaactcg agatagcacg aatgtgagcg
     7681 taccttgcac ccgaccgatt gcagaggtcc tcgagaaatg cctgaacacc ctcgcgcggc
     7741 cgcacgacgc agttttcagc ccaggacggc agtgtggcaa ggttctccgt gtggcgtccg
     7801 tccatatgcg attcactccc gagccggtcg atgggtcgca tctctatctt taggagtgac
     7861 gcaagatgcg aattccctac cccacggatg tcttctgctc ggcgagaggc gtcggcgccc
     7921 cgcgagacgt gcggcgctaa gagggcgcga ccttgtgtcc ggagcatttc gaagcgctcc
     7981 gcttgcaaat ccgccgagcg ccctgagtgc taatatccag cccatcatgg cgttcgcatc
     8041 ggagcaccca gcgcggcccc aactcggtcg tactatgtcg ttcgaggaat gggccgccat
     8101 gcccgaggat gagccgggcg agttggtcga tggtcgtatc gaggaggaag aggcgcccga
     8161 tctcgttcat gcattggtcg tgtcctggct ggttaggatc cttgggacgt ggctcggccg
     8221 aggcgggttc gtattcggct ccgaggcgaa gtatgccctc cgtgcggatc gcggcagaaa
     8281 gcctgatgtc gcggtgtacc tcggctccga cgggcgtcac ctgccgcgac gtggaatcgt
     8341 acgcgtccca cccgatatcg tggtggaggt cgtatcgcct tcaccccgcg acgagcggcg
     8401 tgaccgcatc gagaagatgg acgagtacgc tgcgttcggc ctgcgcttct actggattct
     8461 cgacccgagc ctgcagtcgc tcgaggtgtt cgaactgctc ggcgtgcgtt acgcacgcgc
     8521 agcgcgggcc acagagggcc gcatggatcc ggttccaggt tgccgcgaac tggtgatcga
     8581 gctcgatgac ctctgggcgg agctctcccg gcttggtact ccggacgaat gatcaacggc
     8641 gtgctgccac gcggggcaca gtctcttgtt cggtctgatc tgcatccata gggagtagca
     8701 cgatggccaa tacatccgtg acagttcgcg tgcaggcgta cggcggcaag tttgtcggcg
     8761 acaagatgaa accgacgctg accgtgaaca tcattggcgg cggcacgccg gttctcaatc
     8821 agccgattcc gagtccgagc accggtaccg tcgcgcagcc gttccagcag tgcgcggccc
     8881 gcgacacgat cgtcgtgcag cctccttcgc agaaggcggg tgaatggtgg gtgatccccg
     8941 atccggcgcc ggggcagttg acggtgtcgt tcgatctcga ggttccgtcg ctcgtcgaat
     9001 tcaccgcaac tgccgggccg cagtcggtga cggggatggc gtcgatgttt ctcatccctg
     9061 gcctgcagct taccggagtc gatcctggcc tggtcatcac gctgcatggg ttggccgtct
     9121 tcactccgac gtggacgccg gccacgcccg gtagcgtcgc gatcagcgcg gaagtgacga
     9181 tgatgtgcgg ctgtcccatc accccgcttc cgccgccggc tggcgtcgag gcgtattggc
     9241 ccgacaacga gttcctggtg ttcgcccagt actggggcga cgacgggatc gccgggcagg
     9301 cgggaatgac ctgcggcgcg aacagcacgt acgccggaac gatcaccggc ctcacgccgg
     9361 gcaacacgta caacttcttc gtcgtggccc tgcagcaggc cgaaacgaac gtcggagcct
     9421 cgtccaccat caccttcacg gcttgaccgc cgtacgcaag aacgcgacgt agtagttact
     9481 ttggagcggg atgcctatcg ccgcatcagc agggctccca gcgctgcgag catcgtggcg
     9541 agcaggatca gcgtccagtc accaagagcc gggatgaggg cgcccgtggc gatgccgccg
     9601 ttgccggagc tgtcggcgtt gttcgtgaga gttggatcga attcggtggc gttgacggtg
     9661 gcgccgttgt cgaatgcgcc cgcggcgttg atcgtcgcgg tcacgttgat cgcgacgttc
     9721 gcgccggcgg ccaaggtggc gatcgtgcac ggaagggccg ggcatccggc gccgctgacg
     9781 ctggtgatcg tcaggttcgt tggcgtgtcg gtgacctgga tgttggtggc cgccaacggg
     9841 ccttcgttcg atacctggat cgtgtagctg atcgactgcc cggtaacgaa tggaccggcg
     9901 gtagtcagcg tcttgacgat cgagacgtcc gccgaagcgg tcgctacgcc cccttgtgtg
     9961 tccgtgttgt tcgtcgtgtt cggatcgagc tccgagggcg ttgcggtcgc agtgttgttg
    10021 aaggagccgg ctccgttaat ggccgcggtc acgttgatcg caacgttcgc gccggaggcc
    10081 aggctggcga tcgtgcacgg aagggctggg catccggcgc cgctgacgct ggtgatcgtc
    10141 aggttcgttg gcgtgtcggt gacttggatg ttcgtggccg ccgacgggcc tgcattcgac
    10201 acctggatcg tgtagctgat cgactgcccg gtaatgaatg gcccggcggt ggtcagcgtc
    10261 ttgacgatcg agacatccgc cgaagcggct
//
LOCUS       DATLVW010000004         6859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_413646, whole genome shotgun sequence.
ACCESSION   DATLVW010000004 DATLVW010000000
VERSION     DATLVW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413646"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="VNN25_00105"
     CDS             <1..1804
                     /locus_tag="VNN25_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXH89951.1"
                     /translation="AVIFNSSFFNKDASPAYSLKSAVIDPTREVIVQAPRTLLSYGTT
                     VGTVLWNGSEYVAAYSFAPSPPSNLTQVMLQRISRAGEPVDAPLLLSTVPLGPLTPTV
                     VTSNGHEYLVTLGKSFVRIPIGSRNPTAPAELARLVDAQEGLAFARGRQDFLAVWRET
                     DRSGATIRASRVDAKGRYLDGTGVVIATFPDDVGPPGSYVPATVSVDSDGQNWLVVWS
                     YASHVWGCRVSAAGVMLDTKPIAIAAASGATVRWGGTSWLVVSTNTRQLATTTVTSDG
                     LAGPVKVFDEISADVIVPNGWQAFYTYPLLAFDGEQFVFAAGLETYGGGIDSVAIVTE
                     RLGLSGDPIGGSKFILPPEIGRGNGSSLATNGSQDLIVFESWASGPSFQGLLLNRGHA
                     PVTITIDAPQPIAWDTSWNGNEFIVAANTQDGGVQLKHLSRLGVARDTITLPRYSGEF
                     THGVVFPPSSPARPSTLAGSDLPLGLRTRHDAYAGVSREELAFAGDVADSQLQQVEPA
                     APVLIKAIGDPMGVTLSWQPQDNVLGFEIELRQSDGTDRVVGIAAGSTSSTHIAYGGL
                     TGTTINLRAWNAAGLSAPSPDLQITTPRVRAAAK"
     gene            complement(1862..2509)
                     /locus_tag="VNN25_00110"
     CDS             complement(1862..2509)
                     /locus_tag="VNN25_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89952.1"
                     /translation="MSNQREIAATTTEAADASPAEPAEGSMPDLAAVLALIGQIEAHI
                     PGFIPYDINHVRHVANMTRFAKDVIPKMIATVSSLPSVVGLNAFDVAAGKASLAFDTA
                     MQPIIQALSSLLDGAQFTADSRLARCTGQGLAAYAWMKKTAKGPDGVVLRPYVDDMAL
                     TMKKVLNRRSKPASKPPAPEDAQDSPAPNPDQAKPVDDDDLPEDSRKAPEEAAKE"
     gene            complement(2755..2994)
                     /locus_tag="VNN25_00115"
     CDS             complement(2755..2994)
                     /locus_tag="VNN25_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89953.1"
                     /translation="MCIVNRGCASSIHDRHCHLDDADCPIDDAQWRSWFHIVAPMMRI
                     VVCRYASSIDDVHRRFMLRIVESTMRLEASMIRFP"
     gene            complement(3386..6445)
                     /locus_tag="VNN25_00120"
     CDS             complement(3386..6445)
                     /locus_tag="VNN25_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH89954.1"
                     /translation="MDETTATTADPKLAFEIYFHHARDTNTWTNIALARAASGDSLGG
                     AYMLEVPVPPMFEPLSAFRGARSRTRGARALRPLDPFREWAAQFSDAAALRLQNSTTT
                     DDIQQVLKALVLADRALAIDPGLHEAHFNRGLALEKLGLWNAAVDAYRRSLDTHDTLG
                     WTDETVDRMDHIYRPSVRELWKRGREKLELAGWYQDSSAIARIVRKFPQQSRIVAETE
                     FLGKWAGSVDSGDVLAAERNLALARRVAIAVRKSCGDSLLEDAVRAIDEGSKLPHRRA
                     LSSLVRGHRLYELATASHGAASAIAFDDAGKQFDAAGSPMGDVARYSSIVARFDSRPL
                     SDSLKDLRRIERHLNPSYFNFQALVDSEIATALAERGDVYEALERYERAQKGFEHMRD
                     PASVAKMRVFRAHLLTLMGNAAEGWQVRRPALAEADRSGDPSLLELVLNETIFDERFE
                     RSEETARSLSNVLTRISLQYERPAFRISYTRSFAKQPPVQGEHHSVNDRALSSEAANE
                     LRFANGIRLSVSQPRQAEVLLSYCIQYSRATGRALMLPYVYLYRAIAREAENNEDAAI
                     ADLRRSISLLESRRRKITRIDLRDAWIRIADDVFCELFEIYWRRGDDRRAFASGEERR
                     GLVFRDEAVGVDTSPLSPSTIASRLQPGVAMVVITSSNRHTLMTVIERNRIAMHRLEK
                     PTGFWLMERNRLRTIIQDGHEREAIALAEDLYDSLIYPLGVRTAKVSKLVIVADRPLK
                     DLPFSFLRDRRTGDYLLEQFELLQAPSASLFVQRAAPVPAGRPTTAVTLGEPAFDQRA
                     YPALPTLPAAQAESMNVAQLYPNGRSLIGSQATLRNLAESISVADVVDIAAHTKRSSK
                     EPLLLELLLAANAKGSGATSLRETSALPLKGGSTVVVAGCATAISREPGDLTNFAGSF
                     LAAGASNVVGTLWDVDDDAACAFALRFHRSLSEGNSPSASVRAAQLSMLRSDDVQLRN
                     AKGWSAYQVYTLSASNAQAPTAARRESPPNRTPAPSNFRLWSASTRN"
BASE COUNT         1339 a         2048 c         2093 g         1379 t
ORIGIN      
        1 ggccgtgatc ttcaacagct cgttcttcaa caaggatgcc tcgccggcct actcgctgaa
       61 gagcgccgtc atcgacccga cacgcgaagt aatcgtccag gcgcctcgaa cgctgctttc
      121 ctacggaacg accgtaggca ctgttctctg gaacggctcc gagtatgtcg ccgcttactc
      181 gttcgcgcct tcacctccat caaacctcac ccaggtgatg ttgcagcgaa tcagccgcgc
      241 cggcgagccg gtggatgccc ctctacttct atcgaccgtg cctctggggc cccttacgcc
      301 gactgtggtc acctcgaacg gccatgaata cctggtcact ctgggaaaat cgtttgtccg
      361 tattccgatt ggttcgagga atcctactgc tccggccgaa ctggcccgat tggttgatgc
      421 gcaggaaggg cttgcattcg cccgggggcg gcaggatttc ctggcggtat ggcgcgagac
      481 tgatcgcagc ggggccacaa tccgcgcctc gcgtgtcgat gcaaagggac ggtatctcga
      541 cgggacaggt gtcgtcatcg ctacattccc cgacgacgtg gggcctccag gctcctatgt
      601 tccggcaacg gtctcggtcg acagcgacgg gcaaaactgg ctggtggtct ggtcgtacgc
      661 gagtcacgtc tggggatgcc gtgtctccgc cgcgggtgtc atgctcgata ccaagccgat
      721 tgccattgcc gcagcaagcg gcgcaacggt ccgttggggc ggtacctcgt ggctggtcgt
      781 gagcaccaac acgcgccagc tggccaccac caccgttacg tccgatggtc tcgcgggccc
      841 ggtcaaggtc tttgatgaga tatccgcgga cgtcatcgta cccaacggct ggcaagcgtt
      901 ctacacatac ccgcttcttg ctttcgatgg ggagcagttc gtcttcgctg ccgggctcga
      961 gacgtacggc gggggaatag actccgtcgc catcgtgacg gagaggctgg ggctgagcgg
     1021 cgaccccatc ggcggatcca agttcatttt gccgccggag atcggccgcg gcaacggctc
     1081 gtcgctggcc acgaatggat cgcaggatct catcgtcttc gaatcctggg catcagggcc
     1141 ctccttccag ggtctgctgt tgaaccgagg gcacgctccg gtaacgatta cgatcgacgc
     1201 tccacaaccg attgcctggg atacgtcatg gaacggaaac gagttcattg tggctgccaa
     1261 cactcaggat ggaggcgtgc agctcaagca cctatcgagg ctcggcgttg cgagggacac
     1321 gattacgctg ccgcgctact ccggtgagtt tacacacggt gtggtcttcc cgccctcgtc
     1381 tccggcccgg ccgtcgacgc tggccgggag cgatctgcct ctaggccttc ggacacgaca
     1441 cgatgcctat gcaggcgtat ctcgggaaga gctcgcgttt gccggcgacg tagccgattc
     1501 gcagttgcag caggtggagc cggcggctcc cgtcctcatc aaagcgatcg gcgaccccat
     1561 gggagtcacg ctctcctggc agccccagga caacgtcctc ggcttcgaga tcgagctgcg
     1621 gcaatccgac ggcaccgacc gcgtcgtagg catcgcagcc ggcagcacgt cgtcgacgca
     1681 catcgcgtac ggaggcctga ccggaacgac gatcaacctt cgcgcttgga acgccgccgg
     1741 tctttcagcg ccatcgccgg acctgcagat cacgacgccc cgcgttcgcg cggccgcgaa
     1801 gtagacggtc gatctgagtc cgcggacaac gtcatccgca gatgaaagca gagagtgtga
     1861 actactcctt cgcagcctct tccggcgcct tgcgagagtc ctcgggaagg tcgtcatcat
     1921 cgacgggctt ggcttgatcg ggattcggcg cgggagagtc ctgcgcgtct tccggtgctg
     1981 gaggcttcga tgccggtttg ctgcggcggt tcaaaacctt cttcatcgtc agagccatgt
     2041 catcgacata cggacggagc acgacgccat cggggccttt ggcggttttc ttcatccagg
     2101 cgtaggccgc caacccctgt ccggtgcatc tggcaagacg gctgtcggcc gtgaattgag
     2161 cgccgtcgag caacgacgac agggcctgga tgatcggctg catagcggtg tcgaacgcca
     2221 gggaggcctt ccctgcggcg acgtcgaagg cgttcagtcc aacgaccgag ggaagagaag
     2281 agactgtggc gatcatcttc gggatcacgt ctttggcgaa gcgggtcata ttggccacgt
     2341 gtctgacgtg gttgatgtcg tagggaatga acccggggat gtgcgcttcg atctggccga
     2401 tcagggccag caccgcagcc agatccggca tggatccttc ggcaggttcg gcaggtgatg
     2461 catcggcggc ttcggttgtg gtggcggcga tctctctctg gttgctcata cgatcctcga
     2521 agaatcacgc tggtcggact tgccgtgacc cgcgtgggtg aacgggttga agtacttcaa
     2581 gccatctcga catctcatca gcgacgcgct ggcgagcgag gtcatcgaca gacctattga
     2641 ttgagacgaa actgcctggc tgaaagttac agcggcgcta gagaaggtcg tgagcgcgcg
     2701 gggtggaaag cgtttctggc gatttggcgt gcttttggga ccgattcgtc cagttcatgg
     2761 gaatcggatc atcgaggctt cgagacgcat tgtcgattcg acaatgcgca gcatgaatcg
     2821 acgatgtaca tcgtcaattg acgatgcgta gcgacaaacg acaatgcgca tcatcggtgc
     2881 gacgatgtgg aaccacgatc gccactgcgc atcatcgata ggacaatccg catcatcgag
     2941 atgacaatgc cgatcgtgga ttgatgatgc gcagccacga ttgacgatgc acatcaaaga
     3001 tccttcgatg cgctgcgaag atcgttcgca acgactggaa ggtccttcga tgcgcgtaga
     3061 agacccttcg cagcggctag aagatccttc gcagcggcca aaagatcctt cgcagcggcc
     3121 aaaagatccg tcgcatcgcc tggaagatcg ttcgcatcgc ctggaacgtc cttcgctgcg
     3181 cctggaaggt cgttcgcagc gcgttaacga tccttcgatt cttcgctccg cgcagtcgat
     3241 tcagggtgtc cattgaatgt tttcaaccac ggagacacag aggtcacgga ggtctctgtg
     3301 atctcgccgg cggggtgatc gtcgtcatcg gagcgacgct tggggacgcg ctcacgttcg
     3361 cgcagttcga cggattctgc atcggtcagt ttctggtaga agcactccaa agcctgaagt
     3421 ttgatggcgc tggcgtccgg tttggcggcg actctcgccg agcagccgtt ggcgcctgag
     3481 cattggaagc gcttagggtg tagacctgat aggcgctcca ccccttagcg ttgcgcaact
     3541 ggacgtcatc cgatcgaagc atggaaagct gagcggcgcg gaccgaagct gatggcgagt
     3601 ttccttcgct aagagaacga tggaaacgta gcgcgaatgc gcacgctgca tcgtcgtcta
     3661 cgtcccacag cgttcctact acgttgcttg ctccggcagc caggaaggag ccagcaaagt
     3721 tcgtgagatc gccgggttcc cggctgatcg cggtcgcgca accagcgacg acgacggtgc
     3781 tcccaccttt caaaggaaga gcggatgtct cgcgcaagct ggtcgcgccg ctacccttgg
     3841 cgttcgccgc gagaagtagc tccaggagca atggctcctt gctgctccgt ttcgtgtggg
     3901 ccgcgatatc gacgacatcg gctacggaga tgctttcagc gagattgcgg agcgttgcct
     3961 gcgacccgat cagcgatcgg ccgtttgggt agagctgcgc gacattcata ctctcggcct
     4021 gggcggcagg aagtgttggt agagctggat aggctctctg gtcgaaggcg ggttcgccaa
     4081 gcgttaccgc ggtggttggc cggcccgcgg ggacaggcgc ggctcgttga acaaaaagtg
     4141 aagcgctcgg cgcttgcaac agctcgaatt gttccagcag gtagtctcca gtccttcgat
     4201 cccgaagaaa cgagaacggc agatccttga gcggtcgatc tgcgacgatt acgagttttg
     4261 aaaccttcgc agttcttaca ccgagagggt agatgagcga gtcatagaga tcctctgcca
     4321 acgcgattgc ctcacgctca tggccgtcct gaatgatcgt tcggagccgg tttctctcca
     4381 taagccagaa acctgtaggc ttttccaaac ggtgcatggc aatgcggttc cgctcaatga
     4441 ccgtcatcag cgtgtgacgg ttagaggaag tgatgacgac catcgcgacg cctggctgga
     4501 gccggctcgc gatcgtcgat ggtgaaagcg gagacgtgtc taccccaacc gcttcatccc
     4561 ggaaaaccag ccctcggcgc tcctcgccgc tcgcgaaggc tcgccggtcg tctcctcggc
     4621 gccagtagat ttcgaagagc tcacaaaata cgtcgtccgc gatcctgatc caggcatcgc
     4681 gaaggtcaat tcgggtgatt ttcctccggc ggctttcgag tagcgagatc gaccgccgga
     4741 ggtcggcgat tgcagcgtct tcgttgttct ccgcttcacg agcgatcgct cggtagaggt
     4801 agacgtaggg caacatcagc gctcggccgg tcgcacgcga gtactgaatg cagtaagaga
     4861 ggagcacttc ggcttgacgc ggctggctca cagacagacg gataccgtta gcaaaccgga
     4921 gctcgttcgc tgcttcggaa gacagcgcgc ggtcgttgac cgaatgatgc tccccttgca
     4981 caggcggctg ctttgcgaac gaacgcgtgt agctgattcg gaaagcaggt ctctcgtatt
     5041 gcagcgaaat gcgcgtcagg acgttggaca gcgaccgtgc ggtttcctcg ctgcgctcga
     5101 atcgctcgtc gaagatggtt tcgttgagca cgagctccaa cagcgaaggg tcgccacttc
     5161 gatccgcctc ggccagagcc gggcgacgca cctgccagcc ctccgcagcg ttgcccatga
     5221 gagtcaggag atgggcgcga aagactcgca tctttgcgac cgatgcggga tcgcgcatgt
     5281 gctcgaagcc tttctgcgcg cgctcgtacc tttcaagcgc ctcgtagaca tcgccacgtt
     5341 cggcgagggc cgtcgcgatc tcgctgtcca caagagcctg aaaattgaag tatgagggat
     5401 ttaagtgacg ctcaatcctg cgcagatctt tgagcgagtc gctcaaaggt ctggaatcaa
     5461 acctcgcaac gatggaggaa tagcgcgcaa catcccccat cgggctgccg gccgcgtcga
     5521 actgcttccc ggcatcgtcg aaagcaatag ccgatgccgc accgtgactc gctgtcgcaa
     5581 gctcgtacag tcggtgccct cggaccaggg aagagagcgc acgccggtgc gggagcttgc
     5641 tgccctcgtc tattgctcga accgcatctt cgagcagcga gtcaccgcat gatttacgaa
     5701 ctgcgatcgc cacccgccgc gcgagcgcaa gatttcgctc ggcggcaaga acgtcgccgc
     5761 tatcaaccga ccccgcccac tttcccagaa actctgtctc cgcaacgatg cgcgattgct
     5821 gaggaaactt gcgaacaatg cgtgcaatcg ctgaagaatc ttgataccac cctgcaagtt
     5881 ccagtttctc gcgtccgcgt ttccagagct ctctgacgga tggcctgtag atatggtcca
     5941 tccgatcgac tgtttcatcg gtccacccta gagtgtcgtg ggtgtccaaa gagcggcgat
     6001 aggcatctac cgccgcattc cacaagccga gcttctccaa agcaagacct cggttgaagt
     6061 gagcttcatg aagccccggg tcaatcgcta aagcgcgatc agcaagcaca agggccttca
     6121 ggacctgctg gatgtcgtct gttgtcgtcg aattttgaag gcgaagcgcc gctgcgtcgc
     6181 tgaactgggc cgcccactcc cggaaagggt caagagggcg caacgccctt gctcccctcg
     6241 tccttgatcg agcacccctg aatgcgctta gaggttcgaa catcggcggg acggggacct
     6301 ccaacatata ggccccgcct agggaatctc cactcgccgc tctggcgaga gcgatgttcg
     6361 tccaggtatt ggtgtcgcgg gcatgatgga agtagatctc aaaggcaagc ttagggtcag
     6421 cggtggtggc cgtcgtctcg tccaacgaac ggagccttac gccgtcccgg ctctcctcgc
     6481 gctgcaccag cgtcagccaa tgctcgacac ttgcgttgag gtcaggatcg ccgaggccac
     6541 ctcgcctgcg cactgggcca ggactccagg caacacccgc caggcgcccc tcgcagatcc
     6601 gagtccttga taccgagaga atggatctaa ggtcaaattg gccgtctctg tcacgtctgt
     6661 cggggagacc gcgcgcgctg tgagacgacg tcatggccac cgctaccgcg agcgagacaa
     6721 aggtaaccag gatcgttcga tttctcgccc ccatcctcta cctccgaatg atgctcatga
     6781 acaggagaga cgctggagat ggaagtccct acctgtgagc ggtcagtcca tattttgcag
     6841 actaacgaga ccgtgggag
//
LOCUS       DATLVW010000005         6347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_248263, whole genome shotgun sequence.
ACCESSION   DATLVW010000005 DATLVW010000000
VERSION     DATLVW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248263"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="VNN25_00125"
     CDS             complement(<1..2066)
                     /locus_tag="VNN25_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXH89955.1"
                     /translation="MSKYRNAIRLLFIAVLALPLSAFGANYDVKAFIDTDNSRTTGCS
                     VSLPDGTTSTGFDTILTTSVSVTGTTGTVTAVTRQTCSGGVFGGPVPVDNGWAVGVSP
                     TGDLLIESHLGPNILTMHNVGTPRFVFTTTSGLLSDLLLQPTSTGGGDIIMPHAARDR
                     AVTLLPLRTIVLNGDGSDWAGDVPLALGTANQPALRFISVNGYAGASDFYFNFKIHTN
                     PAAPTAHDDVYDLPTLGGTLSVATLGVLNNDVPNGQSISAVLVDNPLHGALTLNPNGG
                     FVYSNDGAHVTQDQFHYFANGLLPSNIATVTIAMPGTKYVFTSADHVFFTTAIPNSFT
                     VTVTGKPTPALSEDGALPSGITFVDNGDGTATLSGTPGPGTTGIYPLVFHAEKNKPHQ
                     ADQAFTLFIGGCPTVTVTNPAQSSFPAGVPMSVTFTQSAGAPPITWSKIGTLPPGLTF
                     NTSTGVLSGTPTQSGTFPITVTATDASGCSGTGAPYPLTITCPTITVTNPVVNTGTVN
                     VPFSQTFTQSGGFGVTTFSLNSGTLPAGLTLASSGTLSGTPTQSGSFPITVKATDSHG
                     CTGISGTYTLTIGCQVITVTNPVTNTGTVNVPFSQTFTAGNTIGAVTFSLNSGTLPAG
                     LTLSSGGVLSGTPTQTGSFPITVKATDANGCSGVGATYTLTIGCQVITVNNPATNTGT
                     VNVAFS"
     gene            2463..3431
                     /locus_tag="VNN25_00130"
     CDS             2463..3431
                     /locus_tag="VNN25_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH89956.1"
                     /translation="MARPLRLEYPGALWHVTNRGVEQRSIYVDDADRVRFLHLIEETT
                     AEYGWRLLAYVLMSNHYHLLFRTPETNLSRGMKDLDGDYASSFNTRHRRVGHLFQGRF
                     KSHLVDSETYLAEVARYIVLNPVRAHIVEMPGEWRWSSYRATAGLATVPKWLDPGPIL
                     DLFNAGDWNAATHGYREFVAAAIGLDVSPWEDLRAQTFLGGGKFLQWIEQTVAQRKSS
                     SEHLRDQRGFRATTIDSVRATVTAALRLGQWPPKPGTEARLLFALLAERRTNATHAQI
                     GRLLGITSQGAGHVIQAGLRRSTADPEFAARVAATDDRLGSTEAEL"
     gene            4241..6145
                     /locus_tag="VNN25_00135"
     CDS             4241..6145
                     /locus_tag="VNN25_00135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH89957.1"
                     /translation="MRRIFFAIVLLAAPLALAQQRDDKLLDDLDRVRTFNAVAISPDG
                     HHVAWSVQGIGVTTATTGGMDVHLLPGDGDDHYVAWSPDSRSIATVSDGGKNQRQIFV
                     TRVGSTSIRVTSVQGYLAEPQYSPDGKSIAFLFIENAKRAAGPLVAMSRAVGPIEEHI
                     DEQRVAIVELATKKLRIVTPADMYVYHFDWSPDGTHLVTMAAPGSGDNNYWTAKLDVV
                     DVAAATMKPIYTPQLQIAQPKWSPDGKSIAFIEGLMSDEGSTGGDVMVIPANGGAARN
                     VTPGLKASVTSIDWTVAGKITFGEDLAGESALARLDLDSGAIETLWHGAELVTSHDLI
                     GASLAGDGTTSAVIRSGFRSAPDVWAGPIGAWKRLSSTNIASRARWGEARSIRWKSDE
                     FDVQGWLLAPAEVVAGKKYPLIVWIHGGPASASLAHWPDQRAALLSRHGYFVFFPNPR
                     GSYGQGEAFTRANVKDFGYGDLRDVMSGVDAAAASNPIDTARLGIWGWSYGGYMTMWT
                     VTQTQRFKAAVAGAGLANWQSYYGENDIDAWMIPYFGASVYDDPAVYARSAPITFIKN
                     VKTPTLVVVGERDGECPAPQSFEFWHALKTLGVETQLVVYPDEGHRFAKKEHQRDVAQ
                     RLLGWFDKHL"
BASE COUNT         1193 a         1928 c         1984 g         1242 t
ORIGIN      
        1 tggctgaaag cgacgttgac cgtgccggta ttcgtggccg ggttgttgac ggtgatcacc
       61 tggcagccga tggtcagcgt gtaggtcgcg ccgacgccgc tgcaaccatt cgcgtcggta
      121 gccttgacgg tgatcgggaa cgaaccggtc tgcgttggcg tgccggaaag gacgccgccg
      181 ctggagagcg tgaggccggc cggcagtgtg ccgctgttga gcgagaacgt taccgcgccg
      241 atcgtgttgc cggcggtgaa ggtctggctg aaggggacgt tcactgtgcc ggtgttggta
      301 accgggttgg tgacggtgat gacctggcag ccgatggtca gcgtgtaggt cccactgatg
      361 ccagtgcagc cgtgcgaatc ggtagctttg acggtgatcg ggaaggaacc gctctgcgtc
      421 ggcgtgccgg agagcgtccc gctggaagcc agcgtcaagc ctgccggcag cgtcccgctg
      481 ttgagcgaga aggtggtgac gccaaatccg cccgactgcg taaacgtctg actgaacgga
      541 acattgacag tgccggtgtt cacgaccggg ttcgtcacgg taatggtcgg gcaggtgatg
      601 gtcagaggat acggtgcgcc ggtgccgctg cagccgcttg cgtcagtggc tgtgacggtg
      661 atcgggaacg tgccgctctg cgtcggcgtg ccgcttagaa cgccggtgga ggtattgaag
      721 gtgagacccg gtgggagcgt tccaatcttc gaccatgtga tcggaggggc gcccgcgctc
      781 tgtgtgaacg ttaccgacat gggcacgccg gccgggaagg acgactgcgc gggattggtc
      841 accgtgacgg tcgggcagcc gccgatgaag agcgtgaagg cctgatcggc ctggtggggc
      901 ttgttcttct cagcatggaa gaccagcgga tagatgccgg tcgtgcctgg acccggggtg
      961 ccggacagtg ttgcggttcc atcgccgttg tcgacaaacg tgattcccga cggaagcgcc
     1021 ccatcctcgg aaagcgccgg tgtcggcttg ccggtaaccg ttactgtgaa cgagttcggt
     1081 atggccgtcg tgaagaatac gtggtcagcg ctggtgaaca cgtacttcgt gcccggcatg
     1141 gcgatcgtga ccgtggcgat gttcgaaggc aggaggccgt tggcgaagta gtggaactga
     1201 tcctgcgtta cgtgggctcc gtcgttcgag taaacgaatc cgccgttcgg gttgagcgtc
     1261 aaggcaccgt gcagggggtt atcgaccagc actgccgaga tcgactggcc gttcggcacg
     1321 tcgttgttca ggacgccgag ggtcgcgacg gacagcgttc cgcccagggt gggcagatcg
     1381 taaacgtcat cgtgcgcggt gggcgcggcc gggttggtat ggatcttaaa gttgaagtaa
     1441 aagtcgctcg cgccggcgta gccgttgacg ctgatgaacc ggagtgcggg ttgattggct
     1501 gttccaaggg ccagaggtac gtcgccagcc cagtcgcttc catcaccatt gagaacgatc
     1561 gtgcgcaacg gaagcaaggt gaccgcacgg tcgcgtgcgg cgtgcggcat gatgatgtct
     1621 cccccgccgg tggaggtcgg ctggagaagg aggtcggaca gcagccccga ggtcgtcgtg
     1681 aagacgaagc gaggcgtgcc gacgttgtgc atcgtcagaa tgttcgggcc gaggtgtgat
     1741 tcgatcagca gatcgccggt cggtgagaca ccgacggccc atccgttatc gacaggaacc
     1801 ggaccgccga agactccgcc ggagcatgtt tgccgcgtca ccgcggtcac cgttcccgtg
     1861 gtacctgtga cggacaccga ggtcgtgagg atcgtgtcga acccggtcga ggtggtcccg
     1921 tccggcagcg acacggaaca accggtggtc cggctgttgt cggtgtcgat gaacgctttt
     1981 acgtcgtagt tcgcaccgaa ggcggaaagc ggcagtgcga gaacagcgat gaacaaaagg
     2041 cgtatcgcgt tgcggtactt gctcataagg gcagattcct tgagttggct ctcgacatca
     2101 atcgcgcacc agttgacgaa gcagcagctc ccaaccgatg gcggggacga cttggatgaa
     2161 acggataata gcagctagtc cggcgactca ctaattttat ttacgaccga ttctggtgga
     2221 attgggggtg tggggggttg gagaatcagg tctcgaagct cacgctttca aaatggggtc
     2281 aggtctgaag ttgaaagtga ggaaggggga cgtcgtctcc tgatccggtc aacgttcgtc
     2341 actttcaact tcagacctga ccccattttg aaagtgccat cttgaaagca ggccgcaatt
     2401 gctctatgat tctcttccca cgcgcgaccg gtcctcggtc tctcttgcgc tcccctatca
     2461 ccatggcccg ccctctccga ctggagtatc ccggtgcgct ctggcacgtc acgaatcgag
     2521 gcgtcgagca gcggtcgatc tacgtcgatg acgccgaccg cgttcgcttc ctgcacctca
     2581 tcgaggaaac gaccgcggag tacggctggc ggttgctcgc gtatgtgctc atgtcgaatc
     2641 actatcacct gctcttccgc acgcccgaga cgaatctctc gcgggggatg aaggatctcg
     2701 acggcgacta tgcctcgtcc ttcaacaccc gtcatcggcg cgtcggtcat ctgttccagg
     2761 gacggttcaa gagtcatctc gtcgacagcg agacttacct cgccgaggtg gcgcgctaca
     2821 tcgtcctcaa tcccgtacgt gctcatatcg tcgagatgcc gggcgaatgg cgctggagca
     2881 gctatcgcgc aacagccggt cttgcaacgg ttcccaaatg gctcgaccca ggaccgatcc
     2941 tcgacctctt caacgccggt gattggaacg ctgccacgca cggatatcgg gagttcgtcg
     3001 ccgcggccat cgggcttgat gtctcgccgt gggaagacct tcgcgcgcag acattccttg
     3061 gcggaggcaa gtttctgcag tggatcgagc agacagtcgc gcagcggaaa agcagttccg
     3121 agcatctgcg tgaccaaagg gggtttcgcg cgacgacgat cgactcggtg agggcgaccg
     3181 tcacggcagc actcaggttg gggcaatggc ctccgaaacc cggcaccgag gctcgtctcc
     3241 tatttgctct gctcgccgaa cgccgtacga atgcgacaca cgctcagatc ggccgcctgc
     3301 tcgggattac gagccaggga gctggacacg ttatccaagc aggccttcgt cgatcaaccg
     3361 ccgatcctga gttcgcggcg cgcgtcgccg caaccgatga tcggctcggt tccacggagg
     3421 ccgagctctg accatcgctt tcaaaatggg gtcaggtctg aagttgaaag tgacaacgga
     3481 cgagcgctgt ttctccgcca ctcaaactct ggtcactttc aacttcagac ctgaccccat
     3541 tttgaaagtc cagcgtctcc gcgtgaccgt catcggtctc tgacgaagga atatcgatac
     3601 ttgatgaggg aagaggcgac gcttgcactg attcgacgct tgctcgattc ggtggcgtga
     3661 acggtttaac tggtgcgcgt cacccactga ctggtccgcg gcatgactca actcatcacc
     3721 tcgatgactc aactcttccg ctcaatgacc taactcatcc gctggataac ccaactcatt
     3781 cggctggatg actcaactct tccgctcgat gactcaactc atccggctgg atgacccaac
     3841 tcatccgcgt gatgactcaa ctcttccgct cgatgaccca actctttcgg ctgaatgacc
     3901 caactcatcc gcgtgatgac tcatctcatc cgggccacca gtcatagcgt catcgccacc
     3961 agtcatagca tccgcgccac gagttaactc atccgcgcca tgacccaact cattcgcgcc
     4021 atcagtcgag ctgttcgcgc caacagtcaa acgacccgcg cgtgccgggg ctcgttctgc
     4081 gtcatcagtc ggacggttgg gcggatgttt cccagaatca acgttacgct gcttgggatc
     4141 tcgtccacgt tcccgcaatc ctcgcggttc gtcgcgtcca accatgaacc ggcaagtacg
     4201 cagagcggca cgctttccaa cattgctatc cttcactccc atgcgccgaa tcttcttcgc
     4261 gatcgttctc ctcgccgctc ccctcgcgct cgcacagcag cgcgacgaca aactcctcga
     4321 tgatctcgac cgcgtccgga cgttcaacgc cgtggccatc tcgcccgacg gccaccatgt
     4381 cgcttggtcc gtgcaaggca tcggcgtgac gacggccacg accggcggca tggacgttca
     4441 cctgctaccc ggcgacggcg acgatcatta tgtggcgtgg tcgccggact cgcgatcgat
     4501 cgcgaccgtc agcgacggcg gcaagaacca gcgccagatt ttcgtcacac gcgtcggcag
     4561 cacgtccatc cgcgtcacga gcgtgcaggg ctacctcgcg gagccgcaat actcgccgga
     4621 cggcaagtcg atcgccttcc tcttcatcga gaacgcgaaa cgcgcggccg ggccgctcgt
     4681 ggccatgtcg cgcgcggtcg gcccgatcga agagcacatc gacgaacagc gcgtggccat
     4741 cgtcgagctg gcgacgaaga agctgcgcat cgtcacgccg gccgacatgt acgtctacca
     4801 cttcgactgg tctcccgacg gcacgcacct cgtcacgatg gccgctcccg gttccggcga
     4861 caacaactac tggaccgcga agctggacgt cgtcgatgtc gcggccgcaa ccatgaagcc
     4921 gatttacaca ccgcaactgc agatcgcgca gccgaaatgg tcgcccgatg gcaagtcgat
     4981 cgcgttcatc gaaggactga tgagcgacga aggctcgacc ggcggcgacg tgatggtgat
     5041 ccccgcgaac ggcggcgccg cccgcaacgt cacgcccgga ctcaaagcct cggtcacatc
     5101 gatcgactgg acggtagcgg gcaagatcac cttcggcgaa gacctcgcgg gcgagtcggc
     5161 gctggcgcgc ctcgacctcg acagcggagc gatcgagacc ctctggcatg gcgcggagct
     5221 cgtcacgagc cacgacctca tcggcgcgtc gctggccggc gacggcacga cctccgccgt
     5281 cattcgcagc ggcttccgca gcgctcccga tgtgtgggcc gggccgatcg gcgcatggaa
     5341 gagactctcg tcgaccaaca tcgcgtcgcg cgcaaggtgg ggcgaggcgc gttccatccg
     5401 ctggaagagc gacgagttcg acgtgcaggg atggctgctc gcgccagccg aggtggtggc
     5461 ggggaagaag tatccgctga tcgtctggat tcatggcggt ccggccagcg catcgctcgc
     5521 gcactggccc gatcaacgtg cagcacttct gtcgcgccac ggctacttcg tcttctttcc
     5581 gaatccgcgc ggcagctacg gacaagggga agcgttcacg cgcgcgaacg tgaaggactt
     5641 cggctacggc gaccttcgcg atgtcatgtc cggcgtcgac gccgccgccg catccaaccc
     5701 gatcgacacc gcccgcctcg gcatctgggg ctggagctac ggcggctaca tgacgatgtg
     5761 gaccgtgacc cagacgcagc gattcaaagc ggccgtggca ggcgccggtc tcgccaactg
     5821 gcaaagctac tacggcgaga acgacatcga cgcctggatg atcccctact tcggcgcgtc
     5881 ggtttacgac gatcccgccg tctacgcccg cagcgcaccg atcacgttca tcaagaatgt
     5941 gaagacaccg acgctcgtgg tggtcggcga gcgcgacggc gaatgtcccg ccccgcaatc
     6001 attcgagttc tggcacgcgc tcaagaccct cggcgtagaa acacaactcg tcgtctatcc
     6061 cgacgaagga caccgcttcg cgaagaagga acatcagcgc gatgttgcgc agcggctgtt
     6121 gggatggttc gataagcatc tatgaggcag agatcaggag agacagagat cagagatcag
     6181 agatcaggag caagggacac gtgttcggag attggagcgc gatcagagat cagagtttct
     6241 tttgtctctt gtcccttgtc tcttgtctct tgtctcttgt ctcttgtctc ttgtctcttg
     6301 tctcttgtct cttgtctctt gtctcttgtc tcttgtctct tgtctct
//
LOCUS       DATLVW010000006        10714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_579342, whole genome shotgun sequence.
ACCESSION   DATLVW010000006 DATLVW010000000
VERSION     DATLVW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579342"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VNN25_00140"
     CDS             complement(<1..1061)
                     /locus_tag="VNN25_00140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXH89958.1"
                     /translation="MNDSFDFAVIGGGIAGVSIASHLAEHARVALVEMERQPGYHSTG
                     RSVSLFCETYGGPVVRALTRASRRFYFEPPSTFCTAPLVTPRPLLMIASSSQTDALQE
                     FFTSATPGDRVERLSSREAIEAFPLLARSDLVGAALLRETADIDVHELLQGYLRLLRT
                     RGGTFRAATTLTQLARHGDAWTLSTSRGDIRATTVVNAAGAWAGEIGKLAGAMNIGLR
                     PLKRSVALIEQPPGHNMNDWPLLVDAEERFYAKPSPGRLFLSPADETLSAPCDAQADD
                     IDLAEAVDRFERATTIEVKRLVGQWAGLRSFVADREPVVGFDPLVPGFFWLAAFGGYG
                     IQVSPALSFLAASWARGCP"
     gene            complement(1324..2343)
                     /locus_tag="VNN25_00145"
     CDS             complement(1324..2343)
                     /locus_tag="VNN25_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HXH89959.1"
                     /translation="MQYEIPRPYLLFLGEQTSAPHAKTAFGLRDWAPDLCVGEFALPG
                     ATVSTGLPILTPDLAYARGARSLVIGIAPIGGSIPIRWSGVLISALEAGLDLVSGMHT
                     RLEEIPNLAATAERLGRRLINVRQPPAGLPIATGRQRTGKRLLTVGTDCALGKKYTAL
                     SLARGLRSRGIAVDFRATGQTGILIAGGGIPIDAVVSDFAAGAAELLSPDAAADHWDI
                     VEGQGSIFHPAYSGVSLALLHGTQPDVIVMCHEIGRDHILGFPNYPTPSLPDAIELTL
                     VLARRTNRQVRCGGVSLNTSALDDSSAQSVLAEYAKTLRLPVADPIRGGPAFDQLMSE
                     CMSAR"
     gene            2562..4712
                     /locus_tag="VNN25_00150"
     CDS             2562..4712
                     /locus_tag="VNN25_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH89960.1"
                     /translation="MKRIVGTMSALALTVGSTVLAAPVATDSTDAKTGIAGLEEIVVT
                     AQKREERLQDVPIAITVLQGARLDESSAVSLFDAMGNVPGVAITGAQNAAFQGGGAQL
                     TIRGVTAGAPSLAGTSPIAYYLDGVPFGLVKSAVVPDLAPYDLDRVEVLRGPQGTLYG
                     ASAEDGVVRVITNDADLQHFDIKTRLSGGTTEDGGGNYQAAVAVNVPIIDGKLAVRAV
                     VGEESLSGWIDSPVRNNVNDARLETLRLKVNAQPTDELSIGLSAWHSSSKYGGPSLSS
                     DDGTITATEPQPTDIKFNMYGLKIGYDFHSFSLSSMSGYIDYDSDNYFDISPIGPLLG
                     VTFPEGPNFPPLEHNVLNSKVFSEEINLASKQLDAWRWTAGLSYRDAKDQLLQQFIGV
                     GNEDQSKSYAVFGELSRKFFHDQLEWTLGGRYFHDDESTTDLMPPPGIPVDHFTASFN
                     ATTPRVVLSWFAQPNVTAYASYSQGFRSGFGQQPVILEAAPGFPPVQPDKLNNYEIGS
                     KGTLWDGRLSFDAAVYYVKWNDVQQSLQVLYQGASFGAFVNAGSASGAGAEFAVTARL
                     LDGLEVSVNSSWNDLKFDSDVTSADGLLFAKGQRLNRSPEYTAGASVRYTFALGSGGY
                     TGNLAASGNYTSPMSAPPQIGVANGMVFEGSDLVLARASATVKAPAHWSATAFVDNIN
                     NYKRSPLPEFPGIDEWNERVRPRTYGLQFEYHLR"
     gene            4681..5676
                     /locus_tag="VNN25_00155"
     CDS             4681..5676
                     /locus_tag="VNN25_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HXH89961.1"
                     /translation="MGYSSNTISDSQSVEQRIARVRDTARSRLEIALVLLLATTPLPI
                     WAAANAAAMTRPTLKIYVSVDMEGVAGVVSGDQLNPAGFEFERFRGFMTDEAVAAVHA
                     AVDAGATEVVVSDSHGNGENLLVERFPKDVRLVRSFPRRGAMLAGLDASFGAVVFIGY
                     HASTTNSTGVRAHTISSAHFTRVALNGAAVTEAEFNADYAGDLGVPVVFVSGDDAAIR
                     EITGRLGPLDSVVTKTSLGFHAAETLTPAAACDAIYQGVRSAIGHRGQRKPLKIAHPI
                     TVELSFKNYRAAEVLTYLRAIQRTDAHSVRFVGQDMQEVSDFVDFVDFYNPDLTP"
     gene            complement(5682..6158)
                     /locus_tag="VNN25_00160"
     CDS             complement(5682..6158)
                     /locus_tag="VNN25_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HXH89962.1"
                     /translation="MNDDANTSSIPLRPVASDAVKITIGADTFTARWEVDVSPHTCAA
                     FRRLLPYSQKIIHARWSGEACWIPLGTFDLAVGPENPISRPKPGQILFYPAGASETEI
                     LVPYGTARFNSVAGELAGNHFLTIVDGLDRLERLGRDILWHGSRDIRFEAASHRER"
     gene            complement(6313..7029)
                     /locus_tag="VNN25_00165"
     CDS             complement(6313..7029)
                     /locus_tag="VNN25_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXH89963.1"
                     /translation="MDDNPPTLAKIIRSLRVRNAWTLKEMSEKTGIPLSTLAKVEHDR
                     LTLGYDKLLQISQRLKIRMSEFFAGSSDAADGPQMTRRSIGTLDSAVRVESKNYEYFF
                     LCPDLRKKRMLPMAARLRAKTLEEFGDLVRHAGEEFLYVLSGRIEVHTEFYAPVTLET
                     GQCVYIDSTMGHAYLVGAGCEEAMVIGSCSDIDDALLNSLPEAVARAAAEPIVRLLER
                     PTAKRGIAAPARTKARSHRR"
     gene            7236..8984
                     /locus_tag="VNN25_00170"
     CDS             7236..8984
                     /locus_tag="VNN25_00170"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXH89964.1"
                     /translation="MTSTTIRRISHALLLMLPISFATLGWAAVPEFDLVIQGGRIVDG
                     TGGPSYLGDVGIRGARIVYIGPPRPMRAKQVIDAHGQAVSPGFVNMLSQAQESMIVDG
                     REESDLYQGVTLEVMGEGDSMGPLTDQMAARNESRESDIKYPINWRTLGQYLQARERA
                     GTSLNIASFVGATTVRDFVLGEGDVQPSPEQLQRMRALVRQAMEEGALGVSSALIYTP
                     ATFAKTDELAALAAEAGMCGGIYITHMRSEGDRLLEAVDETISIARQSGAPAEIYHLK
                     AGGKSNWPKLDEVIAKVTSARKSGVRITADMYTYTAGGTGLDAAMPPWVREGGPEAWT
                     QRMQSPEVRARVAKEMDDPHAGYENLYLKAGPDGMILAAFKSEKLKPLTGKSLTEVAN
                     MRHESPEDTAMDLVIEDGSRVGVIYFVMSEDNVRKESMLPWMSFGSDGEAPSPEGIFL
                     KSSAHPRAYGNFARLLAKYVRTDKAVTLQDAIHRLTALPAANLSLKDRGLLKEGYFAD
                     IVVFDPNKIQDHSTYVKPHQLASGVKDVIVNGQLALSDGIATGAHSGQFVRGRAWADG
                     KGGGCRASAKDWPWAW"
     gene            9013..>10714
                     /locus_tag="VNN25_00175"
     CDS             9013..>10714
                     /locus_tag="VNN25_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HXH89965.1"
                     /translation="MPSALQADGLRAVTLEDRYARESGTVLLSGTQALVRLLFEQRRR
                     DAAAGLDTAGFVSGYRGSPLGGLDMQLWRERDRLCAARIHFEPGVNEDLAATAVWGSQ
                     QVPLLPQAEAAGVFALWYGKGPGVDRAGDAIKHGNRQGTSRYGGVLVVFGDDHPGKSS
                     TISHQSEQALAANGVPVLYPASVQEYLDLGLHGYALSRHTGTWVGLKCVNETVETTSI
                     VDVDPGRILCRVPPSSFGDSNDIHGRFVFDPLGDEQRHVRRRLPRVHAYVRENALDRV
                     THGGPVHSDAATLGVVAAGKSWLDVIEALDALGLDAARLTTLGIAVYKVALIWPLEPL
                     ALRDFARSCAELLFVEEKAAFLEPQATHHLYNLPANVRPRIVGKHDECGAPLLPSDVP
                     LDPLEIARAIGLRLRRLGKCDLPLEERLKHIERDLEEASARVAGPTARTPYFCSGCPH
                     NSSTRLPEGSLGMAGIGCHTMAIGMNRRTLPPTQMGGEGLNWVGIAPFTGIQHVFQNL
                     GDGTYFHSGLLAIRGAVTAGVNITYKILVNDAVAMTGGQPVEGHLSVAEITHQLRAER
                     V"
BASE COUNT         2080 a         3248 c         3358 g         2028 t
ORIGIN      
        1 atggggcaac cacgtgccca tgatgcagca agaaagctga gtgccggcga aacttgaatg
       61 ccgtagccac caaatgcggc cagccagaaa aagccgggga ctagcggatc gaagcctaca
      121 accggctccc ggtcggcgac aaacgatcgg agccccgccc actgccccac gaggcgttta
      181 acctcgatcg ttgttgcgcg ctcgaagcga tcgactgctt ccgcgaggtc gatatcgtcc
      241 gcctgcgcat cgcagggagc gctcaaggtc tcatccgcgg gcgataagaa gagtcttccg
      301 ggcgatggct ttgcgtaaaa ccgctcttct gcatctacga gcaggggcca gtcgttcatg
      361 ttgtgacctg gcggctgctc gataagggcc accgatcgct tgaggggccg caggcctatg
      421 ttcatggctc cagcgagctt gccgatttcg ccggcccagg cgcccgccgc gttcacaacg
      481 gtcgtcgcac ggatatcacc gcgcgacgtg gatagtgtcc acgcatcgcc atgccgcgct
      541 aattgtgtga gtgttgtagc ggcgcggaag gttccacccc gcgtcctgag gagccgtagg
      601 tatccttgca gtaattcgtg gacgtcgata tcggccgtct cgcgaaggag tgcggcaccc
      661 accaagtcgc tccgtgccaa caaagggaac gcctcgatgg cttcgcggct cgacaggcgc
      721 tcaacccggt ctccgggcgt ggcggaggtg aagaactcct gcaatgcatc ggtctgacta
      781 ctactggcaa tcatcaacag gggacgcggc gtgaccagcg gcgcggtaca gaatgtgctc
      841 ggcggctcga agtagaagcg gcgactagcc cgcgtcaacg cgcgaacgac cggccctcca
      901 taggtttcgc agaatagcga aacggacctt ccggttgagt gatatcccgg ttggcgctcc
      961 atctccacca gcgctacacg ggcgtgttct gcaaggtggc tcgcgattga tacgccggcg
     1021 attccgcccc cgatcacggc aaagtcaaat gagtcgttca tacgttcacg cctcgctgct
     1081 gacgagcggt gcggagttgg ggcgactctt gcaccgccac agccaccaca caccgtaggt
     1141 catcaacggg atgacgtaga tcattgacat cgcgtaagac agtccgcgat agccccgcgc
     1201 gatcaaggtg acgatcccga agcgctgggc acgtcgattg caaagcgtcg tgggcactca
     1261 cgtcggcgcg ccgaaacgcc tcgacctcaa cgctgactcc caccgagcga tgtcccggac
     1321 tcgtcatcga gcgctcatac actcgctcat cagctgatcg aaggcggggc cgccgcgaat
     1381 cggatcggcg accggcaagc gaagggtctt ggcatactcg gcgagcaccg actgagctga
     1441 ggagtcgtcg agcgcggagg tatttagtga gacgccgccg cagcgcactt gacggtttgt
     1501 gcgccgagcc agcacgagcg tgagctcaat tgcgtccggc aagctcggag ttggatagtt
     1561 ggggaaacct aagatgtgat ctcgcccgat ctcgtggcac atgacgatga cgtcgggttg
     1621 ggtcccatgg aggagtgcca aggacacgcc ggaataggcc ggatgaaaaa tggagccctg
     1681 accctcgacg atgtcccagt ggtcggccgc agcatcgggg ctcagcagtt cggcggcgcc
     1741 cgcagcaaag tcggacacca cggcatcgat tgggatgccg ccgccggcga tcaggatgcc
     1801 cgtctggccg gtggcacgaa agtcgacagc aatgcctcgg ctgcgcaagc cgcgcgccaa
     1861 gctgagcgca gtatactttt tgcccagggc gcaatcggtt cccactgtaa gcaatcgctt
     1921 gccggtgcgc tgtcggccgg ttgctatcgg cagaccggcc gggggctggc ggacatttat
     1981 gagccggcgt ccaagtcgtt cagcggtcgc ggcaagattt ggaatctcct cgaggcgcgt
     2041 gtgcatgccg ctcacaagat caaggccggc ctccagtgcg ctgatcagca ctcccgacca
     2101 gcggattgga atcgagccgc caattggggc gataccgatc acaaggctgc gtgcaccgcg
     2161 tgcgtaagcg aggtccggcg tcagtattgg caatccggtg gaaaccgtgg cgccgggcag
     2221 ggcgaactcc ccgacgcaca aatccggcgc ccagtcgcgc agtccgaatg ctgtctttgc
     2281 atggggagcg gaggtttgct cgccgaggaa caatagatag ggtcggggta tttcgtattg
     2341 catcgttccc atgtcctcga tcgtgcgggc ggggcaccac atgggcgagc tgtcgcaagc
     2401 atcgaccgat gatccggccg tcatcgcttt ggttgcctcg aagatcctaa gcgggtataa
     2461 atgtcctgtc cagagaaata ttggctacag gagtcacact aatcctgata gccaccctat
     2521 tgggatcgta ggccagccgg agggatcgac gcatgacaag aatgaaacgg atcgtgggca
     2581 cgatgagcgc tctcgcgctg accgtgggat cgacggtgct tgccgcgccg gtagcgacgg
     2641 attcaaccga tgccaaaacc ggcattgcgg ggctcgagga gatcgtggtc accgcgcaga
     2701 agcgtgagga acgcctgcag gatgtgccga tcgcaatcac ggttctgcaa ggtgcccggc
     2761 tcgatgagtc gagtgccgtg agcttgttcg acgcaatggg caacgtaccc ggcgtcgcaa
     2821 tcaccggcgc gcagaatgcc gcgttccaag gaggcggcgc gcagctgacg attcgaggcg
     2881 tgacggcagg cgccccatcg ctggcgggga ccagtccaat cgcctactac ctggacggcg
     2941 ttccgttcgg tctggtgaag tcagctgtcg tgcctgacct cgccccctat gatctcgatc
     3001 gcgtggaagt gctgcgcggg ccgcaaggca ccttgtatgg tgccagcgca gaggacggcg
     3061 tcgtccgtgt gatcaccaac gacgccgatc tgcagcattt cgacatcaag acccgccttt
     3121 cgggcgggac gactgaggac ggtggcggca actaccaggc cgccgtcgca gttaacgtgc
     3181 ccatcatcga tggcaagctt gcggtccgcg cggtagtcgg cgaggaatct ttgagcggct
     3241 ggatcgacag tccggtgcgg aacaatgtta acgacgcgcg cttagaaacc cttcgcttga
     3301 aggtcaacgc gcagccgacc gacgaactgt cgatagggct ctccgcgtgg cactcgagct
     3361 ccaagtacgg cggcccgtcg ctgtcgtcgg acgatgggac catcacggcg acggaaccgc
     3421 agccgacaga tatcaagttc aacatgtacg gcctcaaaat tggatacgac ttccactcat
     3481 tttccctgag cagcatgtca ggctatatcg attacgacag cgataactac ttcgacatct
     3541 cgcccatcgg cccactgctc ggcgtcacct tccccgaggg tccaaatttc cctccgctcg
     3601 agcacaacgt cctcaattca aaggtcttct ccgaggagat caacctggcg tcgaaacagc
     3661 tcgacgcatg gcgctggact gccggccttt cttaccggga cgcaaaagat cagcttctgc
     3721 aacaattcat cggcgtgggc aacgaggacc agtcaaaatc gtacgcagtg tttggggagt
     3781 tgagtcgcaa gttcttccac gatcaattgg agtggacgct cggcggtcgg tactttcacg
     3841 acgacgagtc gactaccgac cttatgccgc ctccgggcat cccggtcgat cactttaccg
     3901 cgtcatttaa cgccactacg ccgcgagttg tgctgtcctg gttcgcccag cccaatgtca
     3961 ccgcctacgc atcgtattcg caggggtttc gcagcggctt cggccaacag ccggtgattc
     4021 tcgaggcagc tccagggttt cccccggtgc aaccggacaa gcttaataac tacgagatcg
     4081 gttccaaagg gacgctctgg gatggccggc tgtcgttcga cgcggcggtc tactacgtga
     4141 agtggaatga tgtgcagcag tccctgcagg tgctctacca gggcgccagt ttcggcgcgt
     4201 tcgtgaacgc cgggtcggca agcggagccg gcgccgagtt tgcggttacg gcccgcctct
     4261 tggacggact agaagtgagc gtgaattcca gctggaatga cctgaagttc gactccgatg
     4321 tcacgtcggc ggatggtctg ctgtttgcca aggggcaacg gctgaatcgc tcgcccgaat
     4381 ataccgccgg ggcctcagtc cgttatacat ttgccctggg ttcgggcggc tacacaggca
     4441 acctcgccgc ctcgggcaac tacacgtcgc caatgtcggc gccgccgcaa atcggcgtcg
     4501 ctaacggaat ggtcttcgag ggcagcgatc tggtgctcgc ccgcgcgagc gcgaccgtca
     4561 aggcgcccgc gcattggtcc gcaaccgcct ttgtcgacaa catcaacaac tacaaacggt
     4621 cgccgcttcc ggaattccca ggcatagatg agtggaatga gcgtgtgagg ccgcgaacct
     4681 atgggttaca gttcgaatac catctccgat agccagtcgg tggagcagcg gattgcccgg
     4741 gtccgcgaca cggcgcgaag tcgactggag atagcgctcg ttttgctgct ggcgacgaca
     4801 ccgctgccca tctgggccgc ggctaacgcg gctgcgatga cccggccgac gctcaagatt
     4861 tatgtgtccg tcgacatgga gggcgttgcg ggtgtggtct ccggggatca actcaatccc
     4921 gccggctttg aatttgagcg atttcgcggc tttatgacgg acgaggcggt cgcggccgtg
     4981 cacgccgccg tcgacgctgg cgcgaccgag gtggtggtca gcgactccca cggcaatggc
     5041 gagaatctgc tggtggagcg gttccccaaa gacgtgcgac tggtgcgctc gttcccgcgc
     5101 cgcggggcca tgctcgcagg tttggatgca tcgttcggcg ccgtcgtgtt catcggctat
     5161 cacgcttcga cgacgaactc caccggcgtg cgtgcgcaca cgatttcgag tgcgcacttt
     5221 acccgggtcg cgctcaacgg tgcggcggtg accgaggcgg agttcaacgc ggactacgcg
     5281 ggcgatctcg gcgtgccggt cgtgtttgtc tcgggcgacg acgccgctat tcgagaaatt
     5341 accggccgtc tgggccccct ggactcggtg gtcacgaaga cgagcctggg ctttcatgct
     5401 gccgagaccc ttaccccggc cgctgcatgt gacgcgatct accagggcgt ccggtcggca
     5461 atcgggcatc gcggccaacg gaagccgctt aagattgcgc acccgattac ggtcgaacta
     5521 tccttcaaga actacagagc ggccgaagtg ttgacgtacc tgcgcgcgat tcagcgcaca
     5581 gatgcgcact cggtgcgctt cgtgggtcaa gatatgcaag aagtctccga ctttgtcgac
     5641 tttgtggact tctacaatcc ggacttgacg ccttgatgcc tctatctctc ccgatggctg
     5701 gcggcctcga agcggatatc ccgacttccg tgccagagga tgtctcgccc tagccgttcc
     5761 aggcgatcga gaccatcgac gatcgtcaga aagtgattac cggcgagctc gccagcgaca
     5821 ctattgaatc gtgcggttcc gtaggggacc agaatctccg tctcgctggc accggcggga
     5881 tagaagagga tctggcccgg cttcggccgg gaaatcggat tctccggccc gacggcgaga
     5941 tcaaacgtgc ccagtgggat ccagcacgcc tctccactcc aacgcgcatg gatgatcttt
     6001 tgcgagtaag gcaatagacg ccggaacgcg gcacatgtat gcggcgagac gtcgacttcc
     6061 caccgcgcgg tgaaggtgtc ggcaccgatc gtgatcttca cagcgtctga ggcaaccggc
     6121 ctcagcggaa ttgaagaggt gtttgcgtca tcgttcatag atccatcccg gctgcctgtg
     6181 atgccgcatg ggcggcgagc aattctctat acaggacgct tgtatcctac cggagacttt
     6241 actgcaatgg ttatttgagc ggcacggtaa ttcacttcga acgagtgcgc gctccgtggg
     6301 cgacgttggc cgtcagcggc ggtgactgcg cgccttcgtg cgcgctggtg ccgctatgcc
     6361 ccgcttggca gtgggccgct cgagcagcct tacgatgggt tctgcggccg cgcgcgcgac
     6421 ggcctccggt agcgaattca agagcgcatc atcgatatcc gagcagctgc cgatgaccat
     6481 ggcctcctcg catccggcgc cgaccaagta ggcatggccc atggtagagt cgatgtaaac
     6541 gcattgtccc gtctcgagcg tcaccggcgc ataaaattcc gtgtggacct cgatccgacc
     6601 acttaatacg tagagaaact cctcgcccgc atgtcgaacg agatcgccga attcctcgag
     6661 cgtcttcgca cgaagtcggg ccgccattgg aagcatgcgc ttcttgcgca ggtcaggaca
     6721 cagaaaaaag tattcgtagt ttttagactc gacgcgcaca gcgctgtcga gggtgccgat
     6781 actgcgtcga gtcatctgcg gaccatcggc cgcgtccgat gagccggcga agaactccga
     6841 catccggatc ttcagccgct gactgatctg aagcagcttg tcatagccga gtgtcaaccg
     6901 gtcatgctcg accttggcga gcgtcgagag cggaataccc gttttctcgc tcatctcctt
     6961 cagtgtccag gcgttgcgca ctctcagcga gcgaatgatc ttggctaatg ttggcgggtt
     7021 gtcatccatt gtttcgttgt cctcagcaat cttggttcct gcggtgcgat tctgccaagg
     7081 tttcgtccct ccgtgacatc gtaccagcat tggaactcaa cccgaccacg cgccggtcca
     7141 gcccgtgcaa tggtagcacg acagattgac tgttctccat acagacgtta ccgtcctgca
     7201 tggagaaatc gctggcgaca cctacaaggt actgcatgac atcaacaaca atccgcagaa
     7261 tctcgcacgc cttgctcctc atgctgccga tcagcttcgc caccctgggc tgggccgcgg
     7321 tgcctgagtt tgacctcgtg attcagggag gccggatcgt cgatgggacc ggcggcccat
     7381 cctacctcgg agacgtcggt atccggggtg cacgcatcgt ctacataggg ccaccgcgtc
     7441 cgatgcgcgc aaagcaggtg attgacgcgc acggtcaggc cgtctcgccc ggcttcgtca
     7501 acatgctcag ccaagctcaa gagagcatga tcgtagacgg tcgtgaggaa agcgacctct
     7561 atcagggcgt cacactcgag gtgatgggcg aaggcgactc gatgggtccg ctgaccgacc
     7621 aaatggccgc gcgcaatgaa tcgcgcgaat ccgacatcaa atatcccatc aattggcgca
     7681 cgctcgggca gtatctacaa gctagagagc gggcaggcac gtcgctcaac atagcctcat
     7741 ttgtcggcgc cacgaccgtc cgcgacttcg tgctcgggga aggcgacgtg cagccttcgc
     7801 cggaacaact gcaacgaatg cgtgccctcg tgcgccaggc gatggaggag ggcgcgctcg
     7861 gagtctcttc cgccctcatc tacacgcctg cgaccttcgc caagacagac gaactcgcgg
     7921 cgcttgccgc cgaagctggg atgtgtgggg gaatctacat cacccatatg cgaagcgagg
     7981 gagaccgtct gctcgaggcg gttgacgaga ccatctcgat cgcgcgacag agcggcgctc
     8041 cagccgagat ctatcatctg aaggcaggcg gaaaatcgaa ttggcccaag ctcgatgaag
     8101 tcattgcaaa ggtcacctct gccagaaagt ctggtgtacg catcacggcg gacatgtata
     8161 cctatacggc cggcggtacc ggactcgacg ccgcaatgcc gccgtgggtt cgtgaaggag
     8221 gccccgaagc gtggactcaa cgaatgcaaa gtccggaagt ccgtgcccga gttgcgaaag
     8281 agatggacga cccccacgcg ggctacgaga acttgtacct caaggccggc cctgacggca
     8341 tgattctcgc ggcgttcaag agcgagaaac tcaagccgct aaccgggaaa tcgctgaccg
     8401 aggtcgcgaa catgcgccac gaaagcccgg aggatactgc catggacctc gtcatcgagg
     8461 acggcagtcg cgtgggtgtc atttacttcg tgatgtccga ggataacgtg cgcaaggaga
     8521 gcatgttgcc gtggatgagc tttggttcgg acggagaagc gccctcaccc gagggaatct
     8581 ttctaaaatc cagcgcgcat ccacgggcat acggtaactt cgcgaggtta ctggccaagt
     8641 acgtgcgtac ggataaagcg gttactttgc aggatgccat tcaccggctg acagccctgc
     8701 ctgcagcaaa cctatcgttg aaggatcgtg gcctgttgaa agagggctat tttgccgaca
     8761 tcgtcgtgtt cgacccgaat aagattcagg atcactccac gtacgttaag ccccatcaac
     8821 tcgccagcgg tgttaaggac gtcatcgtga acgggcaact cgcgctatcg gatggaattg
     8881 caactggcgc gcattccggg cagttcgtgc gcgggcgggc ctgggcggac gggaaaggcg
     8941 gcggctgtcg cgcctcagcg aaggactggc cgtgggcgtg gtgagccgcc cacgccgtgg
     9001 cgccggactg tgatgccgag cgcgctgcag gccgacggtc ttcgagctgt gacgctggag
     9061 gaccgctacg ctcgcgaaag tggcacggtg cttctcagcg gcacccaagc actcgtccgg
     9121 ctgttgtttg agcagcgccg tcgcgatgca gcggcaggcc tcgacaccgc aggcttcgtc
     9181 agcggttacc gagggtcgcc cttgggcggc cttgacatgc agctctggcg agaacgcgat
     9241 cggctgtgcg ccgcgcgcat ccacttcgag ccgggcgtca atgaagatct tgcggccacc
     9301 gcggtgtggg gctcgcagca agtgccattg ctgccgcaag ccgaggccgc cggcgtcttc
     9361 gcgctttggt acggcaaggg cccgggcgtc gatcgggcgg gcgatgccat caagcatggg
     9421 aaccgtcagg gaacgtcccg gtatggcggt gtcctcgtcg tctttggtga tgaccaccct
     9481 ggcaagagct caacaatttc gcaccagagc gaacaagcac tggcggcaaa cggcgttcca
     9541 gtgttgtatc cggcgagcgt gcaggagtat ttggatctgg gattgcatgg ctacgcgctg
     9601 tcccgacaca ccggtacgtg ggtcgggctc aagtgcgtga acgagacggt ggaaactacg
     9661 agcatcgttg acgtggaccc gggccgcatc ctttgccgcg taccaccatc atctttcggg
     9721 gactccaatg acatccacgg gcgctttgtc ttcgacccgc tcggcgacga acaacgccat
     9781 gtgcgacggc gtcttccgcg tgtgcatgcg tacgttcgag agaacgctct tgaccgcgtc
     9841 acgcacggcg gacctgttca cagtgatgca gccacactgg gcgttgttgc ggccggaaag
     9901 agttggctcg acgtcatcga ggcgctggac gcactcgggc tggatgcggc gcggctcaca
     9961 acacttggga tcgccgtcta caaggtcgcg ctgatctggc cgctggagcc ccttgccctc
    10021 agggactttg cgagatcctg cgccgagctg ctgttcgtcg aggaaaaagc agcgttcctc
    10081 gagccccagg cgacgcacca tctctacaat ctgcccgcca acgtgcggcc tcgaattgtc
    10141 ggcaagcatg acgagtgcgg ggcgcccctc cttccctccg atgtgccgct cgatccgctc
    10201 gagattgccc gcgcgatcgg tctgcgcctt cgccgcttgg ggaaatgtga tcttccgctc
    10261 gaggagcggc tcaagcacat cgagcgggat ctcgaggagg cgagcgcgcg agtcgcaggc
    10321 ccgaccgccc ggacgccgta tttctgttcg gggtgtccgc acaattcgtc gacgcgcctg
    10381 ccggaaggca gcctcggcat ggcggggatc ggctgtcata cgatggcgat cggcatgaac
    10441 cgccgcacgc tgccgccgac acagatgggt ggcgagggtc tcaactgggt ggggatcgcc
    10501 ccgtttacgg gcattcagca cgtcttccag aacctgggcg atggcaccta ttttcattcg
    10561 gggctgctcg ccatccgcgg ggccgtgacg gccggcgtca atatcaccta caagatcctc
    10621 gtcaacgacg cggtggcgat gactggtggg cagcccgtcg agggccatct gtcggttgcg
    10681 gagatcacgc atcagctgcg cgcggaacgc gtac
//
LOCUS       DATLVW010000007         6771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_207396, whole genome shotgun sequence.
ACCESSION   DATLVW010000007 DATLVW010000000
VERSION     DATLVW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207396"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(486..1721)
                     /locus_tag="VNN25_00180"
     CDS             complement(486..1721)
                     /locus_tag="VNN25_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89966.1"
                     /translation="MFKRIVRFSFLSLLMAAPLFPQALTIGPGFSGSPNSSDSAIAAT
                     RTDIDLVSPASATGSVTSVHVYWSSSGCTNAIKIKFFHRTGNTLTMTAERGPFSSAGN
                     DNTFAMSPAVPVQQGDLIGVARVANCGNPGTFFGFPSAGYLVYGSDVTGSVDVASGTF
                     SGSPLALGGTGTATEQLRGYIPVVGSTAGSLGSNFKTSLQLLFGGPESGSITGKLVFH
                     PASTSGTSSDPTVTYNVAAGQVVTYPDIVATFGRSGLGSVDLITTPAATKPVIITRVY
                     NDAGTLGTSGLTEDVIDPSDSRVINSGFTAFLITPVDPSKTRFNIGVRTGFSGATMTV
                     SLRDTNGSVIHTVSKSYQPNWFEQVDSTSFLGGTVIGPNQSILITVSSGSAIVYGATT
                     DNISNDPNIQFAVALFGIA"
     gene            complement(1935..2681)
                     /locus_tag="VNN25_00185"
     CDS             complement(1935..2681)
                     /locus_tag="VNN25_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89967.1"
                     /translation="MRQRNQSSSPSSTSGKRVRSDTSAGKRGRSVQTRKPSARDAVAS
                     PGPSTPPADMSPEEQAAALMAKLDEFQALMPEYQDHDPNDIRRVTGVARFAADLVMPT
                     IALVTSFPPAADRNLFDTEANQLRVRAFDALGPVVQRLNALAAGVQFTRNNGMGEAGS
                     QSLDVFTWGKSYSKRPDAAALRPYVGTMSKVVKKVLNRRKRKASTPTPAQLPPGAQGL
                     LASRVVPSKAAADGVYPDYFNEALDRATKT"
     gene            3355..3777
                     /locus_tag="VNN25_00190"
     CDS             3355..3777
                     /locus_tag="VNN25_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89968.1"
                     /translation="MPGESGLIFLESVLTARESVLEFGESGLNTFESVFKSFESVLIF
                     FESVLKLLEPGLKSGESVLMFLESVLIFFESVFKLEIDQGSVGGSESPDFGQKRAKID
                     EVVPQRTSVIPANAISLRSRCAGQREGRSAVTTRRSAG"
     gene            3988..5898
                     /locus_tag="VNN25_00195"
     CDS             3988..5898
                     /locus_tag="VNN25_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89969.1"
                     /translation="MGHVARCLAVIFVCCSLRAQVIQTIAGGGTNDGQLAAGTPLASP
                     TDVGFDRAGNLYIAESGLHRVRRVDALTKTITTIAGSGQRGFAGDGGTATAALLADPE
                     AVAIDTNGDIYIADVSNNRIRRVNAATGIITTLPVANLNAPVEIALDGAGSLYILDNG
                     HQRILRVNTSTYAVATIAGNGSNGYSGDGSQATAAALNYPRGLTIDGTGNVYIADTAN
                     QRIRRIDAATNMISTVAGNGTVGDSGDGGAALSAALNYPSSVAVDSSGNFYIADADNA
                     RVRRVDGASKIITIVPTAPLSYSIGVAVDTRGALYVTDAGKGSVLTIDAIVAGGSGDG
                     MPATAATLSLPRGLAVDATGSLYIADRFHHRIRRVDATTRIITTVAGTGEPGFSGDGG
                     PALSAQLQTPFAVAFDRAGNLLIADDDNYRIRRVDAATGTITTIAGNGIDGFGGDGGQ
                     ATSASIGEPSGVAVDSDGNVYIADPFNSRIRRVDAKTHVITTFAGSIDASGVAVDRAD
                     NVYIANAGSNTIVKIARTGAITPVAGNGQLRDPVAVAVDAAGNLYIADRGNSRICKLD
                     ATTNAITTIAGTAIEGFFGDGLIATGAQLNRPEGIAVDDNGNVYIADTSNDRVRIVTN
                     AINGTRRRAARH"
     gene            6156..>6771
                     /locus_tag="VNN25_00200"
     CDS             6156..>6771
                     /locus_tag="VNN25_00200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH89970.1"
                     /translation="MLGTGGMGVVYLAAQVEPIRRRVALKVIKPGSASREILARFSTE
                     RQALATMSHPNVAKIFDAGTTAEGDPFFVMEYVNGVRITEYCDTHCLSIRERLELFAS
                     VCDAVQHAHQKGIIHRDLKPSNVLVTTEDGKPVPKVIDFGLAKAGTLEAETLSVTKDG
                     IVLGTPEYMSPEQAGMMSIDVDARTDIYSLGILLYELLVGEPPYD"
BASE COUNT         1335 a         2016 c         2154 g         1266 t
ORIGIN      
        1 gccaccttct ccccatcaaa aaacgatggg gagaaggggc tcgattggaa ggtggcgcga
       61 gatacaggtg tcaggaccct ttgaaatccc gaacagcgca aacgcgaact tgaggttggc
      121 gacaaaaccc tcaatcgagc gccttctccc cccgcgcttt ttgcgggggg agaaggtgcc
      181 gaaggcggat gaggggggat ttgcggcctg cgcgaactca gccaacgccg agccgtttgt
      241 aatcgtgctg ctcgtgtatg gattgcctcg aacggcccct catccggcct tcggccacct
      301 tctccccatc aaaaagcgat ggggagaagg cgctcgattg gaaggaggcg cgagatacaa
      361 gtgtctcgtt caacatcacg aacactctca atcgagcgcc ttctcccctc gtgcttttcg
      421 aggggagaag gtgccgaagg cggatgaggg gggatttgcg gctcgcgcga acaagtgtca
      481 gaactttacg caatcccgaa caacgcgacc gcgaactgga tgttcggatc gtttgagatg
      541 ttgtcggtcg ttgctccgta cacgatggcg ctgccgctgg agacggtgat caggatcgac
      601 tgattggggc cgatcactgt tccgcccagg aatgaagtcg agtcgacctg ttcgaaccag
      661 ttcggctggt agctcttgct gaccgtgtgg atgacgctgc cgttcgtgtc gcgcagcgag
      721 accgtcatcg tggctcccga gaaaccggtg cggacgccga tgttgaagcg cgtcttcgac
      781 ggatcgaccg gtgtgatgag aaaggctgtg aaaccggagt tgatgacgcg cgaatccgac
      841 ggatcgatca catcctccgt caatcccgac gttcccaacg ttccggcgtc gttgtagacc
      901 cgcgtgatga tcaccggttt cgtggcggcc ggagtggtga tgagatcgac gcttcccagt
      961 ccggaacggc cgaacgttgc cacgatgtcg ggatacgtca cgacctgtcc ggcagcaacg
     1021 ttgtaggtga cggttgggtc cgacgatgtg ccgctggtgc tggccggatg gaaaacgagc
     1081 ttgccggtga tcgagcccga ctccgggccg ccgaagagga gctgcagcga ggtcttgaag
     1141 ttcgacccga ggctgccggc ggtcgagccg acgacaggga tgtaaccgcg caattgctcg
     1201 gtggccgtgc ccgtgccgcc gagcgcgagg ggcgaaccgg agaacgtgcc gctggcgacg
     1261 tcgacgctgc ccgtcacgtc cgagccatag acgaggtagc cggcggacgg aaagccgaag
     1321 aaggtcccgg gattgccgca attcgccacc cgcgcaacgc cgatgagatc tccctgctgc
     1381 acaggtaccg ccggcgacat cgcgaacgta ttgtcattgc cggccgatga gaagggtccg
     1441 cgctcggccg tcatcgtcag tgtgttcccg gtgcgatgga agaacttgat cttgatcgca
     1501 ttcgtgcagc ctgacgatga ccagtaaacg tgcaccgagg tcaccgaacc ggtcgcgctc
     1561 gcgggcgaaa cgagatcgat gtcggttcgc gtcgccgcga tggcgctatc gctcgagttc
     1621 gggctgccgg agaagcccgg gccgatggtt aaggcctgcg gaaagagtgg tgccgccatc
     1681 agaagagaca ggaaagagaa tcggacgatg cgcttgaaca tgagagagcc tccgcgcgca
     1741 tgattccata ttgttgctgg cagcgagtgt gatgcagcca acgtttgggt caggtctaaa
     1801 gtctaaagtg ccgacgcata tcctgccgcc gtgccggcac cagtcttagg cactttagac
     1861 tttagacctg acccggaaaa ccgccgcccc ccgagagcac cggacgaagg gcggcggcga
     1921 acgatcacta cgaactacgt cttcgtagcc cgatccaacg cctcgttgaa gtagtcggga
     1981 tagacaccat cagcggcggc cttggaaggg acgactcttg aagcgaggag accctgggca
     2041 ccggggggca actgcgcggg ggtgggggtt gatgccttcc gcttgcggcg gttgagcacc
     2101 ttcttcacca ccttcgacat cgtgccgacg taggggcgga gggcggcggc atcgggccgc
     2161 ttcgaatacg acttgcccca ggtgaacacg tcgagactct gggatcccgc ctcgcccatc
     2221 ccgttgttgc gggtgaactg cactccggcg gcgagagcgt tgagacgctg cacgaccggg
     2281 cccagcgcgt caaatgcacg cacccgcaac tggttcgctt ccgtgtcgaa gaggttccga
     2341 tcggcggcgg gtggaaagga cgtgaccagg gcgatggtcg gcatcaccag gtcggcggcg
     2401 aagcgagcga ccccggtcac gcggcggatg tcgttcggat cgtgatcctg atactcgggc
     2461 atcagtgcct ggaactcatc gagcttggcc atgagtgcgg cggcctgctc ctcgggggac
     2521 atatcagccg gcggcgtgga agggccgggg gatgcgacgg cgtcccgggc ggacggcttg
     2581 cgggtctgaa ccgagcggcc acgcttgcct gcgctcgtgt cgctacgaac gcgcttgccc
     2641 gaggtggaag aaggactgct gctttggttg cgttgtctca taacatctcc ggatctcata
     2701 gccacgcctg ctggacatgc gcgtggggct ggatgttgag gtacttcgac tggtttactg
     2761 accgtcagcg acgcgctgcc ggccgggaac ttcggtgctc agaatgtttg acgcatcagc
     2821 ccggcaggaa gtttctgggt ggtttcccgg cctcggcgtt tcacgctgcc gggagcgggt
     2881 tgccgcaccg ggggatgagg ttggcggctc cgcatgtcgt ccataggttt ctccgtcgct
     2941 gccggccact acctggatcg tcgtacgtga aaaacgcagg acgctggtgg ccgtgctctg
     3001 aatgtttgac gcagcagccc ctcggaaggt tccggaaccg gtttcgtaga gtcgcggact
     3061 ggcggttccg gctcgccgat ctgcgaggtt gccgggttgc ctcatcgcga tgatgcgaga
     3121 gtcgttgtcc gcgataacgg atgactcatc gacgggccgc gaagcggcaa acggacgtca
     3181 cgaagcaatc acgaatcttc cagaccagca acgtcgacgt ccgggccccc gcgaaccgcg
     3241 ttgccatctt cgtcccgtga gtgcggagtc aaacgtctcg agtccggact cgagaccgat
     3301 gagtcggtac tcacacgtct ctagtcggta ctcacgtcat ccgagtccgt actcatgccc
     3361 ggcgagtccg gactcatatt tctcgagtcg gtactcactg cgcgtgagtc ggtactcgag
     3421 tttggtgagt ccggactcaa tacgtttgag tcggtattca agtcttttga gtcggtactc
     3481 atattctttg agtccgtact caaattgctc gagcccggac tcaaatccgg tgagtcggta
     3541 ctcatgttcc tcgagtccgt actcatattt tttgagtcgg tattcaaatt agaaatcgac
     3601 caagggtccg tcggagggtc ggaatcacct gattttggtc aaaaacgggc caaaatcgac
     3661 gaagtggtgc cgcaacggac ctcggttatt cccgcgaacg caatttcatt gcgctcccga
     3721 tgcgccgggc agcgcgaggg aagatccgcc gtcacaaccc ggcgcagcgc cggctgaaaa
     3781 gtcacggatg cacgcgctga ggacgtcgat ccgccgcgcg tcgaggtcgg gcaacggagc
     3841 aatagcagca atgccgcggc ggaggaggcg accaccggcg actacgcgta ttaggcatgg
     3901 tccggcctca ctcgatggcg tgagcgcaat caccgcccgc gctacgccag cgaccaatga
     3961 atatctctat tccagaagaa gggcaaaatg ggccatgttg cgcgttgcct cgccgtgatt
     4021 ttcgtctgct gttccctgcg tgcgcaagtg attcaaacga tcgccggcgg cggcacgaac
     4081 gacggtcagc ttgcggcggg taccccgctc gcctcgccga cagacgttgg ttttgatcgc
     4141 gccggcaatc tctacatcgc ggagtcgggc ttgcatcgcg tcagacgcgt cgacgcgctg
     4201 acgaaaacga tcacgacgat cgccggcagc ggacagagag gctttgcggg cgatggcggg
     4261 acggcgaccg cggcgctgct cgctgatccc gaagcggtgg ccatcgatac gaacggcgac
     4321 atctatatcg cggacgtgtc gaacaatcgc attcggagag tcaatgcggc aacgggcatc
     4381 atcaccactc ttcctgtcgc gaatctcaac gcgccggtcg agatcgcgct cgacggtgca
     4441 ggcagtctct acatcctcga caacggccat cagcgaatcc ttcgcgtcaa cacgtcgaca
     4501 tacgctgttg ccacgatcgc cggtaacgga tcgaacggct attccggcga tggcagtcag
     4561 gccacggcgg cggcgctgaa ttacccacga ggtctcacga tcgacggcac cggaaatgtg
     4621 tacatcgccg ataccgcgaa tcagcgcatt cgacgtatcg acgcggctac gaacatgatc
     4681 agcacggtcg ctggcaacgg aacggttggt gactcaggcg atggcggcgc ggcgctcagt
     4741 gcggcactaa actatccatc gagcgtcgcc gtcgactcat cagggaactt ctacattgcc
     4801 gacgcggaca acgcgcgcgt gcgccgagtc gacggcgcgt cgaagatcat cacgatcgtt
     4861 cccactgcgc cgctcagtta ctcgatcggc gtcgccgtcg atacgcgagg agctctctac
     4921 gtcacggatg cggggaaagg cagtgttctg acgatcgatg cgatcgtggc cggaggcagc
     4981 ggcgatggca tgcctgcgac ggctgcgact ctctcgctgc cgcgcggact ggccgtcgac
     5041 gcgaccggct ctctttacat cgcggatcgc ttccaccatc ggatccgtcg tgtggatgct
     5101 acgaccagga tcatcaccac cgtcgccggc acaggcgaac cgggattctc cggcgatggt
     5161 ggcccggcgt tgtccgcgca actgcagact ccattcgccg tggcctttga tcgtgccggt
     5221 aacctcttga ttgccgacga tgacaactac cgcatccgcc gagtcgatgc ggcgaccggc
     5281 accatcacga ccattgccgg gaacgggata gacggatttg gcggcgacgg aggccaggcg
     5341 acgtcggcat caatcggcga gccgtccggt gtggccgtcg actccgacgg caacgtctac
     5401 atcgccgacc catttaacag ccggattcgg cgggtcgacg ccaaaacgca cgtcatcacc
     5461 accttcgccg gatcgattga cgcttcgggt gtggccgtcg atcgcgcgga caatgtctat
     5521 atcgccaacg ccggctccaa tacgatcgtg aagatcgcac ggacaggcgc gatcacgccc
     5581 gtagccggca atggccaact ccgcgatccg gtggctgttg ccgtcgacgc ggcggggaat
     5641 ctctatatcg cggaccgggg caacagtcgc atttgcaaat tggacgcgac gacgaacgcc
     5701 atcacaacga ttgccggaac ggccatcgaa ggcttcttcg gtgacggcct gatcgcaact
     5761 ggcgcgcagc tcaatcgccc ggaggggatc gccgtcgacg acaacggtaa tgtctatatt
     5821 gccgacacga gtaatgaccg cgtacgcatt gtgacgaacg ccatcaatgg aacgcgccgc
     5881 cgggctgcac ggcattgacc atgtcagcaa cacaaggttg ccgcctccgg aattagattc
     5941 tcgagaggag attcatcgtg tcgacacgtg tgcgctgcat cgagtgcagg aaagagttca
     6001 cggcgtcggc agaagttgtc gaccgttgcc cagattgccg gcaagacgct gagccacctg
     6061 ccacggaagg tacgaccgtc gttatcgaaa cgacacaagg caacggccag caaaccgctt
     6121 ttcctggctc cattggccca tatcgagtcc tcgaagtgct tggtaccggt gggatgggag
     6181 tcgtctatct cgccgctcaa gtcgagccga tccgccggcg cgtggcattg aaggtcatca
     6241 agccgggatc ggccagccgc gagatcctgg cgcgtttttc gacggagcgc caagcgctcg
     6301 ctacgatgtc gcaccccaac gtggcgaaga tcttcgatgc cggcacaacg gcagaggggg
     6361 acccattctt cgtcatggag tacgtcaatg gtgtgcggat cacggaatac tgcgacacgc
     6421 actgcctctc gatccgggaa aggctggagc tcttcgcatc cgtgtgtgac gcagtgcaac
     6481 acgcgcatca gaagggaatc atccatcgcg atctgaagcc ttccaacgtg ctcgtcacga
     6541 ctgaggacgg caagccggtg ccgaaagtga tcgactttgg tctggcaaag gccggcaccc
     6601 tggaggcgga gacgttgtcc gtcacgaagg atggaatcgt cctcggcacg cccgaataca
     6661 tgagtcccga gcaggccggc atgatgtcca tcgacgtcga tgcgcgaacc gacatctatt
     6721 cactcggtat tctcctctat gagctcctgg tcggcgagcc gccgtacgac c
//
LOCUS       DATLVW010000008         6234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_207425, whole genome shotgun sequence.
ACCESSION   DATLVW010000008 DATLVW010000000
VERSION     DATLVW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207425"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VNN25_00205"
     CDS             complement(<1..190)
                     /locus_tag="VNN25_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXH89971.1"
                     /translation="MQVGGSGKIKSDINVTPLVDVVLVLLIIFMVITPMLQKGVDVKL
                     PSAGNTKERKEEGKKVTVA"
     gene            complement(192..638)
                     /locus_tag="VNN25_00210"
     CDS             complement(192..638)
                     /locus_tag="VNN25_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXH89972.1"
                     /translation="MATHRWHSSVGKFTAASLTSDINVTPLVDVCLVLLIIFMVVTPM
                     LQKGVPVNLPVTEEPEKTPDTEKQLQISVKADGTVYLGSLPVIKQQMQSELEKIHERT
                     PDREIAVKGDKLVKYGAVLDALKACREVGFNNVGLIAQPKKSPGAV"
     gene            complement(616..1275)
                     /locus_tag="VNN25_00215"
     CDS             complement(616..1275)
                     /locus_tag="VNN25_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXH89973.1"
                     /translation="MSTAAKGIVIVMIIMSIYSIWVMIERFITFNQAKNQSLKLLGAL
                     SNVLTKGDYQQAIDITKKYKQSHLAKVISAGLLEFEAARRDKRYSDPEVAVEAARQGM
                     DRTAMITVAELKENLGVLATIGATAPFVGLFGTVIGIMHAFGKMATSGGGIASVSAGI
                     AEALLTTAFGLFVAIPAVWAYNYFQNRVDRFTVEMSNSGSEMSIYLLKEAGTHGNSSV
                     A"
     gene            complement(1353..2090)
                     /locus_tag="VNN25_00220"
     CDS             complement(1353..2090)
                     /locus_tag="VNN25_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH89974.1"
                     /translation="MFETTVVESRKQKVGIQRVMTLPVSVALHVVVVVGLVIGAIWNV
                     DFPMNSPAQAAQYSVAAAPPPPPPPPPPPKAAQATQVVKPVEIPKNVQEMAPTLVPEK
                     VIPVAAAPSNAGVEGGVEGGVEGGVPGGVVGGVIGGVITPAPQPKPDAPLRVGGDVKA
                     PVVINRVEPLYPEVARKARISGIVIVECTISKGGDVTDIHVLKPLPFGLDQAAAEAVK
                     RWKFKPGTLNGQPVDVIFNLTVNFKLN"
     gene            complement(2123..2947)
                     /locus_tag="VNN25_00225"
     CDS             complement(2123..2947)
                     /locus_tag="VNN25_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89975.1"
                     /translation="MKKVLCLIAALTLALSSLGCTKIRARMEIKAANEAYQKEDWSTA
                     LQHYKVARQIDPSFDDLTRLVGYSEIGLYIPDDKTPKNEAHADAAILELNNYLQKKPD
                     DRIARDALINMYLNANRTTQAIDYFVGYLKTHPADLEAVKSIATLYAKQGDFNKSLEW
                     YEKITLLDAKNPESFYIFGVVCYEKVSKNMAGADPAQKMDLINKGKGALQHAIDMKPD
                     YAEAMAYLNLLWRQQALVDAITDPVKAQADVAQADAIRNQTMQILNARKAAGAKKS"
     gene            complement(3260..3964)
                     /locus_tag="VNN25_00230"
     CDS             complement(3260..3964)
                     /locus_tag="VNN25_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HXH89976.1"
                     /translation="MGTHTGPILEGRGDTDYERYVRVPELLELQKPAELLAHPDERLF
                     QITHQAAELWLHHVDYEITRAAELLGAGDVSLASDLLDRCARIINLLRQQIVILETMA
                     PADYHVVRTSSLGRGSGGESPGFAKLLTVGKRLWPLFEALLAARKVTVIEIERAPREH
                     RELFRLVQAMLDYDEAFLNWRYSHMRLAFRVIGSKVMSLKNVPAAQLEQGTREPLFPE
                     LWETISLLTQEFKPTY"
     gene            complement(4117..5319)
                     /locus_tag="VNN25_00235"
     CDS             complement(4117..5319)
                     /locus_tag="VNN25_00235"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HXH89977.1"
                     /translation="MPFATIDEAAELYRRGEVVIIVDDEDRENEGDLCIAAEKITPEA
                     INFMARFGRGLICLALTEERCAELDLPLMVEHNTSNYGTAFTVSIEARGRVTTGISAR
                     DRAETVRVAIDPRTRPADLLRPGHIFPLRAKKGGVLKRAGQTEASVDLATIAEMMPAS
                     VICEIMNEDGTMARMPDLQAFAIKHGLKIISVADIIKYRMQTEKHVHSLASPRMPTPY
                     GDFRVHLYKSDITGEEHVALVMGEMGEQDEVLVRVHSSCLTGDVFHSARCDCGDQLDR
                     AFELIAKEEKGVILYLLQEGRGIGLLNKLKAYELQEQGHDTVQANEKLGFPPDIRDYG
                     TGSQILRDLGVRKIRLMTNNPAKYVAIEGFGLEIVERVPLEIAPSNDTRKYLEAKKAK
                     LGHMLELV"
     gene            complement(5419..>6234)
                     /locus_tag="VNN25_00240"
     CDS             complement(5419..>6234)
                     /locus_tag="VNN25_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89978.1"
                     /translation="LEAAVVEAKGGKGNNHGAALAYAELISPAGEPFVPVSVYVPASA
                     GVAADGADTIFGAVEDASGKRVTMFEEPAKLTASRTDFFVDKTLTLEPGKYTAVVGLA
                     KAGQPVLVTTGPIEVTGPSKEAVGTSRLILSDNVYELGTAEPPKSPFAFGKLKIVPKG
                     NLVFKSSDELNYFVEVNNPGIDTATNMPKLQYKLDLTGGPDKKTISAPLQEAAALPLT
                     GTPGPGHYAILSAIPLAEVKPALKPGDYTLKMKIVDMVSKQSYTVEQSFKVSG"
BASE COUNT         1130 a         1855 c         1935 g         1314 t
ORIGIN      
        1 ccgccaccgt gaccttcttg ccttcttcct tacgctcttt ggtgtttccg gcgctgggaa
       61 gcttgacgtc cacacccttc tgcagcatgg gcgtgatgac catgaagatg atgaggagca
      121 ccaacacgac gtcgacgagt ggcgtgacgt tgatgtcgga cttgattttg ccgctgccgc
      181 ctacttgcat gtcagaccgc tcctggagat ttcttcggct gcgcgatgag accgacgttg
      241 ttgaaaccaa cctcgcggca agccttgagt gcgtcgagca ccgcgccgta cttgacgagt
      301 ttgtcgcctt tgaccgcgat ctcccggtcg ggcgtgcgct catgaatctt ttcgagctcg
      361 gactgcatct gctgtttgat cacgggcaat gagccgaggt acaccgtgcc gtcagccttg
      421 accgagatct gcagctgctt ttccgtgtcc ggggtcttct ccggctcttc cgtcaccgga
      481 agattcacgg gcacgccttt ctgcagcatg ggcgtgacga ccatgaagat gatcagcagg
      541 acgagacaga cgtccaccag gggcgtaacg ttgatgtcgg acgtcagcga cgccgcggtg
      601 aactttccga cagagctatg ccaccgatga gttgccatgg gtaccggctt ccttcagcaa
      661 gtagatggac atctccgagc cggagttcga catctcgacg gtgaaacggt cgacccggtt
      721 ctggaagtag ttgtacgccc agacggccgg gatggccacg aagagaccga acgcggtggt
      781 cagcagtgct tcggcgatac cagccgacac ggaggcgata ccgccgccgg acgtggccat
      841 ctttccgaac gcgtgcatga tgccgatgac cgtgccgaag agaccgacga acggcgcggt
      901 ggcaccgatg gtggccagaa cgccaaggtt ctctttcagc tcggcgacgg tgatcatcgc
      961 ggtgcggtcc ataccctggc gggctgcttc gacggccact tcgggatcgc tgtagcgctt
     1021 gtcgcggcgc gctgcttcga actcgagaag accggccgag atgaccttgg ccaggtgcga
     1081 ctgcttgtac ttcttggtga tgtcgatggc ctgctgataa tcacccttcg tcagcacgtt
     1141 cgaaagcgcg ccgaggagct tgagtgactg attcttggcc tggttgaagg tgatgaaacg
     1201 ctcgatcatc acccagatcg agtagatcga catgatgatc atgacgatca cgatgccttt
     1261 tgccgcggtc gacattgaaa cccacaacgc tccgagactc atatccatga tgcagttccc
     1321 tccaatgact tccgggcaga ccggacgttc ttttagttca gtttgaaatt gaccgttagg
     1381 ttgaagatga cgtccaccgg ttgcccattg agagtccccg gcttgaattt ccagcgtttg
     1441 acggcctcgg ctgcggcctg atcgagaccg aacggcagcg gcttgaggac gtggatgtca
     1501 gtgacgtcgc cgcccttgct gatcgtgcac tcgacgatga cgatgccgga gatgcgcgcc
     1561 ttgcgggcga cctccgggta caggggctcg acgcgattga tgacgacagg cgctttgacg
     1621 tcgccgccga cgcgcaacgg agcatctggc ttcggctgag gagccggggt gatcactccg
     1681 ccgatgacac cgccgacgac accgcccggc acgccacctt cgacaccgcc ctcaacgccc
     1741 ccttcgacgc ccgcattgct cggcgcggca gcgaccggga tcaccttctc aggcacgagc
     1801 gtgggtgcca tttcctgaac gttctttgga atctcgaccg gtttgacgac ctgcgtcgcc
     1861 tgcgccgctt tcggcggcgg tgggggcggc ggaggcggcg gaggagccgc agcgaccgaa
     1921 tactgggccg cctgcgcggg tgagttcatc ggaaagtcga cgttccagat cgctccgatc
     1981 acgagcccca cgaccacgac aacgtggaga gcgactgaca ccggaagcgt catgacgcgc
     2041 tggataccga ctttctgttt cctggattca acgacggtag tttcgaacat ggtcttcgcc
     2101 tgtatgtaag aacgcgatcg cgctaactct tcttcgcgcc agcggccttt ctggcgttga
     2161 ggatctgcat agtctggttg cggatggcgt cggcctgggc gacgtcggcc tgcgctttca
     2221 ccggatcggt gatcgcatcg accagagcct gctggcgcca cagcaggttg agatacgcca
     2281 tcgcctcggc gtagtcgggc ttcatgtcga tggcgtgttg caacgccccc ttgcccttgt
     2341 tgatgaggtc catcttctgc gcgggatcgg cgccagccat gttcttcgac accttctcgt
     2401 agcagacaac accgaagatg tagaacgatt ccgggttctt ggcgtcgagg agcgtgatct
     2461 tctcgtacca ctccagcgac ttgttgaagt caccctgctt cgcgtagagc gtcgcgatcg
     2521 acttgaccgc ttcgaggtcg gcgggatggg ttttgaggta gccgacgaag tagtcgatgg
     2581 cctgggtggt gcggttggcg ttgagataca tgttgatcaa cgcgtcgcgc gcgatgcggt
     2641 catcgggctt cttctgcaga tagttgttga gctcgaggat ggcggcgtcg gcgtgtgctt
     2701 cgttcttcgg cgtcttgtca tccgggatgt acaggccgat ttccgagtag cccacgaggc
     2761 gcgtgaggtc atcaaagctc ggatcgatct ggcgcgctac cttgtagtgc tggagcgcgg
     2821 tggaccaatc ctctttctga taggcctcgt tggctgcctt gatctccatc cgtgcccgga
     2881 tcttggtgca gccgagcgag gaaagggcga gagtcagtgc cgcaatcagg cacaaaacct
     2941 ttttcatgaa atgtctaact ccaaacgtag tggtacgtcg cggatctgga aacggaagcg
     3001 agctgtcagg ccgtccgggc ggattgggcg acccctcccg tgagatgacg cgcgactata
     3061 ccgatcgact tttgcaccgt caaggcgttc ctaacacctg ataccgaaag ttttagttgc
     3121 attccgcgcc tgttattcat gcagctcgtc cggaggttgg acacgccaca ccgcggagaa
     3181 gaaaccatct cgtgatcatg cgtcatcgcg gcgtccttgt aacacacgga tgccctcgaa
     3241 gctacgcaaa acatgctcat caatacgtgg gcttgaattc ctgagtcagc aagctgatgg
     3301 tttcccatag ttcggggaac aacggctccc tcgttccctg ctccagttgg gccgcgggaa
     3361 cgtttttcag cgacatcact ttcgatccga tcacgcggaa ggccaggcgc atgtgcgagt
     3421 accgccagtt caggaaggcc tcgtcgtagt cgagcatggc ctgcacgagg cggaagagct
     3481 ctcgatgttc ccgtggagcc cgctcgatct cgatgacggt gaccttgcgc gcggcgagga
     3541 gcgcctcgaa aagcggccag agccgcttgc cgacggtcag cagcttggcg aaaccgggcg
     3601 actctccccc gctgccccgt cccaacgatg aggtgcggac gacgtggtag tcggccggcg
     3661 ccatcgtctc caggatcacg atctgctgcc gcaacagatt gatgatacgt gcgcagcggt
     3721 cgagcagatc ggaggccaga ctcacgtcgc cggcgccgag cagctcggcg gcacgggtga
     3781 tctcatagtc gacgtggtgc agccagagct ccgccgcctg gtgtgtgatc tggaaaagcc
     3841 gctcgtccgg atgcgcgaga agctcggccg gtttctgcag ctcgagcagc tcgggtaccc
     3901 gaacgtagcg ctcgtaatcg gtgtcgccgc ggccctcgag aatggggcct gtgtgtgtgc
     3961 ccaagcgtgc tccctagctt cctgattgtc gatgttttgg ttgcggttga attgccttcg
     4021 gcgttctcag ttatcagtga tgccgccaac ttgaagctgt tggatacccg acaacgccgt
     4081 aggcaactaa ccgacaaccg acaacgccgc aggcaatcaa accagttcca gcatgtgccc
     4141 gagctttgct ttcttcgcct cgaggtattt gcgcgtgtcg ttcgacggcg cgatctccag
     4201 aggtacgcgc tcgacgatct ccagtccgaa gccttcgatc gcaacgtact ttgccggatt
     4261 gttcgtcatc aggcggatct tgcggacgcc gaggtcgcga aggatctgcg aaccggtacc
     4321 gtagtcacgg atgtcggggg ggaagccgag cttctcgttg gcctgaaccg tgtcgtgacc
     4381 ctgctcctgc agctcgtagg ccttcaactt attcagcaat ccgatcccgc gcccctcctg
     4441 caatagatag aggatgacgc ccttctcttc cttggcgatc agttcgaagg ctcgatcgag
     4501 ctggtcgccg caatcgcagc gcgcggagtg gaagacgtcc ccggtcaggc aggaggagtg
     4561 aacgcggaca aggacctcgt cctgctcgcc catctcaccc atcaccaggg cgacatgctc
     4621 ttcgccggtg atgtcgcttt tgtacagatg cacacgaaaa tcgccgtagg gcgtcggcat
     4681 gcgcggcgac gccagcgaat gcacatgttt ctcggtctgc atccgatact tgatgatgtc
     4741 ggcgaccgag atgatcttca gaccgtgctt gatggcgaat gcctggaggt cgggcatgcg
     4801 cgccatcgtg ccgtcttcgt tcatgatctc gcagatcact gaagcgggca tcatttcggc
     4861 gatggtggcg agatcgacgg atgcctccgt ctggccggcg cgcttgagga ccccaccctt
     4921 cttcgcgcgc agcggaaaga tgtggccggg gcgcagcaga tcggcaggtc gggtacgcgg
     4981 atcgatagcc acgcgcacgg tctcggcacg gtcacgtgcg gagatgccgg tcgtcacgcg
     5041 gccgcgcgcc tcgatcgaga cggtgaacgc ggttccgtag ttggacgtgt tgtgctcgac
     5101 catgagcggc aggtcgagct ccgcgcagcg ctcttcggtg agcgccagac agatcaaacc
     5161 ccgtccgaag cgagccatga agttgatcgc ttccggcgtg atcttctctg cggcgatgca
     5221 gaggtcgcct tcattctcgc gatcttcgtc atcgacgatg atcacgacct cgccgcggcg
     5281 gtacagctca gcggcttcat cgatcgtggc aaagggcatg gtgtgattgt aagggagtgg
     5341 ggggtgggat gtagggagtg ggagagaaat caagatttat ctcccacacc caactcccca
     5401 caccccaccc cccaattcct atcctgaaac cttgaaactc tgctccaccg tgtacgactg
     5461 cttcgacacc atgtcgacga tcttcatctt cagggtgtag tcgcccggct tgagcgccgg
     5521 cttcacctcg gcgagaggaa tcgcgctgag gatggcgtag tggcccggac cgggcgtgcc
     5581 ggtgagcggc agcgccgcgg cttcctgcag cggggcgctg atggttttct tgtccggacc
     5641 gccggttaga tcgagcttgt actgcagctt cggcatgttc gtggcggtat cgatgccggg
     5701 gttgttgacc tcgacgaagt agttcagttc atcgctgctc ttgaaaacca gattgccctt
     5761 cggcacgatc ttcagcttgc caaatgcgaa gggcgatttc ggaggctcgg cggtaccgag
     5821 ctcgtagacg ttgtccgaga ggatgaggcg cgacgtgccg accgcttcct tcgacggacc
     5881 ggtcacttcg atcggaccgg tcgtcaccag aacgggttgc cccgctttgg cgaggccgac
     5941 gacggccgtg tactttcccg gctcgagcgt cagcgtcttg tcgacgaaga agtcagtgcg
     6001 cgaggccgtg agcttcgccg gctcttcgaa catcgtcacg cgcttgcccg acgcatcttc
     6061 gaccgcgccg aagatcgtgt cggcaccgtc tgcagcaaca ccggcggatg cggggacgta
     6121 aaccgagacc ggaacgaacg gctcgccggc cggcgaaatg agctccgcgt acgcgagtgc
     6181 ggcgccgtga ttgttgcctt tgccaccctt cgcctcgacg actgcggctt cgag
//
LOCUS       DATLVW010000009         3696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_124656, whole genome shotgun sequence.
ACCESSION   DATLVW010000009 DATLVW010000000
VERSION     DATLVW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124656"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VNN25_00245"
     CDS             complement(<1..282)
                     /locus_tag="VNN25_00245"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXH89979.1"
                     /translation="MGELTEKIRTELTAAMKARDAERLSTLRMLQSSFKYQQIELGHE
                     LSDEEAMTVIRKAVKQRLDSIEQYTKGNRPELADKERREMELLKTWLPPE"
     gene            321..893
                     /gene="pyrE"
                     /locus_tag="VNN25_00250"
     CDS             321..893
                     /gene="pyrE"
                     /locus_tag="VNN25_00250"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXH89980.1"
                     /translation="MPLDLPALLRETGALLSGHFRLSSGLHSPGYVQCALLLEHPRNA
                     RAIGEALAARVAAMKPAKIVAPALGGVIIGYAVAEALGLPSIFTERKDGAMTLRRGFV
                     IAPDEPVVIVEDVVTTGKSTRETAAVVEALGGRVVGFASILNRSGTPNPFDAPYEALM
                     ELNLETYDEAGCPLCARGVSLDAPGSRFSR"
     gene            973..1443
                     /locus_tag="VNN25_00255"
     CDS             973..1443
                     /locus_tag="VNN25_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXH89981.1"
                     /translation="MTDKVITIDGESCELELEREGSRFRVAEHAIELVAVHGNEAELR
                     VDGRTHLVPFIIDGTEVSFAYDGEIWIADVTVKGARTRARHADHSLSAPMPGVVLKIL
                     VAVGDDVTKGVPLLILEAMKMEHQITAPRDGIVTAIHCKEGEMVQSGLDLVTLT"
     gene            1440..2378
                     /locus_tag="VNN25_00260"
     CDS             1440..2378
                     /locus_tag="VNN25_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXH89982.1"
                     /translation="MKITIDHVHIVEVGPRDGLQNEKVTIPTDAKIDYITALADAGLT
                     TIEAGAFVSPKWVPQMADTAEVYRNIPKDPGVDYPVLVPNMRGLDRAIEAGVKSIAIF
                     TAASETFNQRNINMTIDESFINYAPVAARARAEGMRVRGYVSTAFGCPYEGDVAPEKV
                     LEVSARLLDLGCYEVSVGDTIGVGTPVQVQGVVGILLQVIPASKLAMHFHDTRGTALA
                     NSLAALEMGIPTFDASSGGLGGCPYAPGASGNLATEDLVYMLEKMGIDTGVDLSRLVA
                     ASQIIGPYLDHPLPGKYLQSCSRAAATAPASTATVQ"
     gene            2382..2813
                     /locus_tag="VNN25_00265"
     CDS             2382..2813
                     /locus_tag="VNN25_00265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXH89983.1"
                     /translation="MPESPRFENGWFVVPWQVIFRDIDAFGHVNNAIYLTYFEWARAQ
                     LWFALTGAAGVPSDIGFIVARAEIDFKLQIGMEPIDICVRIGAIRNTSFDTLYEIRKR
                     STGQVAATGMVVVVLFDWKAQTKVPVSDALRQKVRALQQET"
     gene            2923..3621
                     /locus_tag="VNN25_00270"
     CDS             2923..3621
                     /locus_tag="VNN25_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89984.1"
                     /translation="MITGFNTDIKHNEKVYHIQTEDKGLQNPYIESLVYVGGEILASK
                     KTSYAEQATTGVDEKWIGSLMEQQHRTMIAAIKRGRFDAPADITKSTPRPTLAQPVPG
                     VAEPAPIAIDEEKTLDQVIIDYLASEAESEHLELSLLNSTEFFAGTPVELRVAAKKSL
                     SQSPIAAASIEVKVISTVEPPRVIFRGKTTADGTAIVRCDIPAFAEGTAAIIISAQSP
                     IGNDEVKQLVKRKK"
BASE COUNT          691 a         1120 c         1197 g          688 t
ORIGIN      
        1 ctcgggagga agccaggtct tgagcagctc catttcgcgc cgctctttgt cggcgagctc
       61 ggggcggttg cctttcgtgt actgctcgat cgaatcgagg cgctgcttga ccgctttgcg
      121 gatcacggtc atcgcttctt cgtcgctcag ctcgtggccc aactcgatct gctgatactt
      181 gaacgacgac tgcagcatcc gcagcgtcga gagccgctcg gcatcgcgcg ccttcatggc
      241 cgcggtgagc tcggtgcgaa tcttctctgt cagttcaccc atttgcgctt gctcctcacg
      301 ccattctatt ctcgcccgcc atgccactcg acctgccggc acttctccgc gaaacgggcg
      361 cgctgctcag cggacacttc cgtctctcgt ccggacttca cagcccgggc tacgttcagt
      421 gcgcgcttct gctggaacat ccgcgcaacg cacgggcgat cggcgaggcg cttgcggcga
      481 gggtcgctgc gatgaagccg gcgaagatcg tcgcgcctgc acttggcggc gtgatcatcg
      541 gatatgccgt tgccgaggcg ctgggattgc cgtcgatctt cacggagcgc aaggatggag
      601 cgatgaccct gcggcgagga ttcgttatcg cgcctgacga gccggtggtc atcgtcgagg
      661 atgtggtcac cactgggaag tcgacgcggg agacagctgc ggtagtcgaa gcgcttggcg
      721 ggcgggtggt gggatttgcg tcgatcctca atcggagcgg gacgccgaat ccgttcgatg
      781 cgccgtatga ggcgctgatg gaactgaacc tggagacgta cgacgaggcg ggatgtccgc
      841 tttgcgcgcg cggggtgtct ctcgatgcgc ctgggtcacg gttttcgcgg tagatcacgc
      901 gaagccgcga agacgcgaaa aggaaaagga cctccttcgc ggcttcgcgt ctacgcgcga
      961 tagactccca ccgtgaccga taaagtcatc accatcgacg gggaatcgtg cgagctggag
     1021 ttggagcgcg aaggaagccg ttttcgcgtg gccgaacatg cgatcgagct cgtcgccgtg
     1081 cacggcaacg aagctgagct acgcgttgat ggccgcacgc acctcgtccc gttcatcatc
     1141 gatggcaccg aggtcagttt tgcgtacgac ggggagatat ggatcgctga tgtgactgtg
     1201 aaaggcgctc ggacgcgcgc acgccacgct gatcattcgc tgagtgcgcc gatgcccggt
     1261 gtcgtgctga agattcttgt cgcggtcggc gatgatgtga cgaaaggagt tcctctcctg
     1321 atcctcgagg cgatgaagat ggagcatcag atcactgcgc cgcgcgacgg catcgtcacc
     1381 gcgattcact gtaaggaagg ggagatggtg cagtccggtt tagacctcgt gacgctgaca
     1441 tgaaaatcac catcgatcac gtccatatcg tcgaggtggg gccgcgcgac ggcctgcaga
     1501 acgagaaggt caccatccct accgatgcga agatcgacta catcacggcg ctcgccgatg
     1561 ccggtctgac gacgatcgaa gcaggcgcct tcgtcagccc gaagtgggtg ccgcagatgg
     1621 cagacacggc cgaggtctat cgcaacatcc cgaaggaccc gggcgtcgac tatccggtgc
     1681 tggtgccgaa catgcgcggg ctcgatcgtg cgatcgaggc aggcgtgaaa tcgatcgcca
     1741 tcttcaccgc ggcgtcggag acattcaatc agcgcaacat caacatgacg atcgacgaat
     1801 cgttcatcaa ctacgcgccg gtggctgcgc gtgcgcgcgc tgaggggatg cgcgtgcgtg
     1861 gctacgtctc gacggcgttc ggctgtccgt acgaaggcga cgtcgcgccg gagaaagtcc
     1921 tcgaagtctc cgcccgcctt ctcgacctcg gctgctacga ggtctcggtc ggcgacacga
     1981 tcggcgtcgg aacgccggtg caggtccagg gcgtcgtcgg aatcctcctg caggtgattc
     2041 ctgcctccaa gctggccatg cactttcacg acacgcgcgg aacggcgctc gcgaattcgc
     2101 tcgctgcgct ggagatgggc attccgacgt tcgatgcatc gtcgggggga ttgggcggat
     2161 gcccctacgc gccgggtgct agcggcaacc tggccaccga ggatctcgtc tacatgctgg
     2221 aaaagatggg catcgatacc ggcgtcgatt tgagccggct cgtggccgcg tcgcagatca
     2281 tcgggccgta tctcgatcat ccgctgcccg ggaagtacct gcagtcctgc tcgcgcgcgg
     2341 cagcgaccgc gcctgcatcg accgcgacgg tacaatgagc cgtgcctgag tcgcctcgct
     2401 tcgaaaacgg ctggttcgtc gtcccctggc aggtgatctt tcgcgacatc gacgccttcg
     2461 gccacgtcaa caacgccatc tacctcacgt acttcgagtg ggcgcgcgcg caactctggt
     2521 ttgcgctgac gggtgcggcc ggcgtgccct ccgacatcgg attcatcgtc gcccgcgccg
     2581 agatcgactt caaactccag atcgggatgg agccgatcga tatctgcgtc cggatcggcg
     2641 cgatccgcaa cacgtcgttc gatactctct acgagatccg caagcgttcc acgggccagg
     2701 ttgccgcgac aggaatggtc gtcgtcgtgc tgttcgactg gaaggcgcaa accaaagtac
     2761 cggtcagcga cgcgcttcgg cagaaagtgc gcgcgctgca gcaagagacg tgagccgctt
     2821 gcgactggtg tgcttatcgg acttgcccga aaccgggcag caaagcatct gctaaactcc
     2881 gagacttcga gacagaacac tgtttcgcgt cggagcgtgc ccgtgatcac cggcttcaac
     2941 acggacatta agcacaacga gaaggtctac cacatccaga ccgaggataa gggactccag
     3001 aatccgtaca tcgagtcgct tgtctacgtc ggtggggaga ttctcgcctc gaagaagacc
     3061 tcatacgcag agcaggccac aacgggcgtc gacgagaagt ggatcggcag cctgatggag
     3121 cagcagcacc ggacgatgat cgccgcgatc aagcggggac gattcgatgc gccggccgac
     3181 atcacgaagt cgacgccgcg gcccaccttg gcccagccag tcccgggcgt cgccgagccg
     3241 gctccgatcg cgatcgacga agagaagacg ctcgatcagg tgatcatcga ctatctggcc
     3301 tccgaggccg agagcgagca cctcgagctc tcgctgctga acagcacgga gttcttcgcc
     3361 gggacgccgg tggagttgcg cgtagcagcg aagaagagcc tttcgcagag cccgatcgct
     3421 gccgcctcaa tcgaggtcaa agtgatttcg acggtggagc cgccgcgagt catcttccgc
     3481 gggaagacta ccgccgacgg cacagccatc gtgcgctgcg acatccccgc gttcgccgaa
     3541 ggcaccgcgg cgatcatcat ttccgcccaa agcccgatcg gcaacgatga ggtgaagcag
     3601 ttggtgaaga ggaagaagtg agttttgcgg gagtcggttt tggggagtcg gggtcggagc
     3661 tgcgtgcgtc ggttttcggg ggtcgggagt cggagc
//
LOCUS       DATLVW010000010         8652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_496887, whole genome shotgun sequence.
ACCESSION   DATLVW010000010 DATLVW010000000
VERSION     DATLVW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496887"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /gene="atpD"
                     /locus_tag="VNN25_00275"
     CDS             complement(<1..1153)
                     /gene="atpD"
                     /locus_tag="VNN25_00275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXH89985.1"
                     /translation="MANEETKTQRVGKVIQVIGPALDIEFEEGHLPAIYNAIRIVDNG
                     ELGKVPIDVIAEVANHIGENQVRCIAMKPTDGMVRGMKAIDTGGPIMVPVGRETLGRI
                     LNVLGEPVDNKGPVNAETTLSIHRQPPRFDEQSTNIEMFETGIKVIDLLEPYTKGGKT
                     GLFGGAGVGKTVLIMELINNVAKEHGGFSVFAGVGERTREGNDLWLEFAETGVIVPGD
                     PSKSKAALIYGQMTEPPGARLRVGLTGLTVAEHFRDAEGQDVLLFIDNIFRFTQAGSE
                     VSALLGRMPSAVGYQPNLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATA
                     FAHLDATSVLSRQIAELGIYPAVDPLASTSKILDPRILGDEHYNTARAVQ"
     gene            complement(1168..2028)
                     /gene="atpG"
                     /locus_tag="VNN25_00280"
     CDS             complement(1168..2028)
                     /gene="atpG"
                     /locus_tag="VNN25_00280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXH89986.1"
                     /translation="MPSTIDIRRRIRSVKNSQQITKAMKMVAAAKLRRAQERMFAARP
                     YAAGLRKVLSSVSTRVDISAHPLLQSREPERNVVVVVVTADRGLCGAFNANVIRAAQN
                     FIREQSFESVSVLTIGRKGGDFFKRRPIPVRQNVMVAQALSLGVAHEIADSLIKDFIE
                     EKIDGVYVVYNEFKSIIAQHVRAERLLPIARDFEEGPEQGGVDYLYEPGPEQILSDLL
                     PKHIEFQLYRVLLESAAAEQGARMTAMEAATKNASDMIEHLTLTYNRIRQAAITKEII
                     EIVSGAAAAQ"
     gene            complement(2035..3573)
                     /gene="atpA"
                     /locus_tag="VNN25_00285"
     CDS             complement(2035..3573)
                     /gene="atpA"
                     /locus_tag="VNN25_00285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXH89987.1"
                     /translation="MAVDLKAEEITEIIRQQLSGIGRGVDVSEVGTVVSVGDGIARVY
                     GLDRVMAGELVQFPHDIAGLALNLEEDNVGVVLLGEASAIKEGDVVKRTGKIMSVPVG
                     PALVGRVVNPLGVPIDGKGPINTTEFYPIERLAPGVVDRKPVKEPLQTGLKAVDAMIP
                     IGRGQRELIIGDRQTGKTAVAVDTIINQKGKDVICVYVAIGQKKSTVAQVVRTLEENG
                     AMDFTIVVNASASDPAPMQYLAPYGGCAMAEYFLYNGQHALIIYDDLSKHAAAYREIS
                     LLLRRPPGREAYPGDVFYLHSRLLERASKMADEKGGGSLTALPIIETQAGDVSAYIPT
                     NVISITDGQIFLETDLFHSGVRPAINVGISVSRVGGSAQVRSMRSVSGTLRLDLAQYR
                     ELAAFAQFGSDLDKATQSQLNRGRRLVEILKQGQYQPMRVALQTASVYAGVKGFLDNL
                     AVESVRQFETALHQYLSDEKGDVLDRLETATKFDADLEGALRTAIQEFKAQYVKDNAN
                     ALAA"
     gene            complement(3621..4154)
                     /gene="atpH"
                     /locus_tag="VNN25_00290"
     CDS             complement(3621..4154)
                     /gene="atpH"
                     /locus_tag="VNN25_00290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HXH89988.1"
                     /translation="MIRRFARPYARAIMDTVQSPEKAAALRDEMTSFEKVRQSSRDLQ
                     QMYANPGIDFDSKHKVTAAIAGHLGLSELAVRLLDVLIQNRRINDLAAIVAGLAAMIR
                     DATGTVAAEVRSASELSAKEQSELRAMLERKVGANVDIDVTVDPDLIGGFVAKIGSEV
                     YDASVSGKIEKFRASLA"
     gene            complement(4151..4804)
                     /locus_tag="VNN25_00295"
     CDS             complement(4151..4804)
                     /locus_tag="VNN25_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HXH89989.1"
                     /translation="MRRLILIITLLLVPAVLFAQKHGNDAGNVAQGAEKVSHEEAHPN
                     EAHGGELGPPKTYFGIPGWILKLVNMLLFFGVLGWLIGGPVKNALAARRVQIQTDAEE
                     ARARRAKADQLAGDIQARLTQIEADVRGIQERAQVEGERQKRELIAAAEAEAQKILQS
                     ARNEVESRLKHARHELTEFAGELATQRAEQILREKVTDHDRERLFEESVREVEGART"
     gene            complement(4804..5238)
                     /locus_tag="VNN25_00300"
     CDS             complement(4804..5238)
                     /locus_tag="VNN25_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HXH89990.1"
                     /translation="MQINLTPDYSLLAIMVIFILNYLIVSRFFLRPINQVIEAREEEV
                     RSANDLYEQSMARFSEATSLMENQLHAARHAAADVRDHFRRDAAAYRTSIVDKTHNEA
                     NAIVNEADERLKQDVVEARAKIVRESEALAQLAAERILGRAV"
     gene            5269..5922
                     /locus_tag="VNN25_00305"
     CDS             5269..5922
                     /locus_tag="VNN25_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXH89991.1"
                     /translation="MRTAPNPEAAVVYHKGKNVVTSPVVRGVPDKTSTETYISVLERA
                     DDFFRGVAAAQPQSLQCGRGCSLCCYGIFEIGSGDVPILAEGLEKLHHKRRAMIIRRA
                     REIVSSSAHPDLRDCTPAEKAAFFERTASTPCPNLNERGECLMYEHRPLVCRTFGLPL
                     RDGDRYIGDICELNFRDASQPQREKAAWDLQWEDALGAEDEYTIPEAIVLIARMRGW"
     gene            complement(6045..6551)
                     /locus_tag="VNN25_00310"
     CDS             complement(6045..6551)
                     /locus_tag="VNN25_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXH89992.1"
                     /translation="MSSWVGAVSGTLVVVVAAVLVAALGFVLILRGIRTPGVKKGMGN
                     DMKNKSGELNGFLDRGSSFHGELEFEDTMRIDGRFNGKIMSKNELIVGESAHIEGDVH
                     VGRIAISGTIVGKIVAAQSVQIHRNGKVYSDIDTPALIIEEGAIFQGNCVMGDKKPAT
                     VTNIASKA"
     gene            complement(6526..7401)
                     /locus_tag="VNN25_00315"
     CDS             complement(6526..7401)
                     /locus_tag="VNN25_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXH89993.1"
                     /translation="MNQRKVLGRGLGALIPQRQEQPPAPVEAHATQGLAEIPISQIQP
                     NPYQPRKTFNEASIEELARSVREHGIVQPLVVTRAGDKYKLIAGERRFRAAQKAGLTT
                     VPALIKEMMQEGDALEIALIENIQREDLNPIEEALAYSQLHDEFQLTQEEISKRVGKE
                     RSTVANFLRLLKLPDPVKKLLASGQLSMGHARALLAIESARKQEQLADRVVRKNLNVR
                     QTEMLASESSPKAVEKKEKEKDVFTRDAEDKLQRTLRTKVEIDRRRRGGVIHIRYGSE
                     DELIRVVDELMGRRR"
     gene            complement(7401..8168)
                     /locus_tag="VNN25_00320"
     CDS             complement(7401..8168)
                     /locus_tag="VNN25_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH89994.1"
                     /translation="MTKILAIANQKGGVGKTTTAINLGASVAACERRVLLVDLDPQAN
                     ATSGIGILDPERSMYDVLIEKMPLADIILPTEIPTLFVAPGSVDLVAAEVELREAIGR
                     EYYLKRVLDPVARDYDYVFIDLPPSLGLLTVNGLTAAHSVLVPLQTEYFALEGVSQLL
                     ATIERVQGAVNPGLEIEGIVLTMYDDRMNLTRQVAEEVRTHFGEKVYRTVIPRNIRLS
                     EAPSFGKPIILYDIRSRGSEAYVSLAREFIQRAENVI"
     gene            complement(8165..>8652)
                     /locus_tag="VNN25_00325"
     CDS             complement(8165..>8652)
                     /locus_tag="VNN25_00325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HXH89995.1"
                     /translation="VANLDDHIAEAVAAAGILKPQGEVLDFGSGGGLPAIPMAIVSPA
                     TRFHLVESDKKKWAFLKHIVRACELNAVVYGDRLARAVMQFPSHLQFSLVTSRAVGNP
                     EEWVPSLGKWMAPEGRVALFQGSPEVPIIRGFTKTDVFTLPRGTSNYLVTLAFHVEHP
                     A"
BASE COUNT         1564 a         2599 c         2706 g         1783 t
ORIGIN      
        1 cctggacggc gcgggcggtg ttgtagtgct cgtcgccgag gatgcggggg tcgaggatct
       61 ttgaagtcga ggcaagcgga tcgacggccg ggtaaatgcc aagctcggcg atctgacgcg
      121 agagaaccga agtcgcgtcg aggtgggcga aggccgttgc cggtgcagga tcggtgagat
      181 cgtccgcggg aacgtagatg gcctgcacgg aagtgatcga cccgcgcttg gtggatgtga
      241 tgcgctcctg caactcgccc atttccgtcg ccaggttcgg ctggtaaccg acggcgctgg
      301 gcatgcggcc gaggagggcc gagacttctg atcctgcctg cgtgaagcgg aagatgttgt
      361 cgatgaacag gagaacgtcc tgtccttcgg catcgcggaa gtgctcggcg accgtcaggc
      421 cggtgagacc gacgcggagg cgcgctcccg gcggctcggt catctgtccg tagatcagcg
      481 ccgctttcga cttcgacgga tcgcccggca cgatgacgcc ggtctctgcg aactcgagcc
      541 agagatcgtt gccttcgcgc gtgcgttcgc cgacgccggc gaaaacggag aagccgccgt
      601 gctctttggc cacgttgttg atgagctcca tgatgagaac ggtcttgccg acgccggcgc
      661 caccgaaaag acccgtctta ccgcccttgg tgtacggctc aaggagatcg atgaccttga
      721 tgccggtctc gaacatctcg atgtttgtcg attgctcgtc gaaccgcggg ggctgacgat
      781 ggatcgagag cgtcgtctcg gcgttgaccg gacctttgtt gtcgaccggc tcgccgagga
      841 cgttgagaat gcgcccgaga gtctcgcgtc cgacgggaac catgatcggg ccgcccgtgt
      901 cgatcgcctt catgccgcgg accattccgt cggtcggctt catggcgatg cagcgaacct
      961 ggttctcacc gatgtgattg gctacttcgg cgatgacgtc gatggggact ttgcccagct
     1021 cgccgttgtc gacgatgcga atggcgttgt agatcgccgg aaggtgacct tcctcgaact
     1081 cgatgtcgag cgcaggcccg atgacctgaa tcacttttcc gacgcgctgg gttttggttt
     1141 cttcgtttgc catgtcttct ccgttgtcta ttgcgctgcg gcagcgcccg agacaatctc
     1201 gatgatctct ttggtgatgg ccgcctgacg gatgcggttg taggtgagcg tcaggtgctc
     1261 gatcatgtcc gacgcgttct tcgtggccgc ttccatcgcg gtcatgcgcg ctccctgttc
     1321 ggcggcagcc gactccagga gaacccgata aagctgaaac tcgatgtgct tcggcaggag
     1381 gtccgaaagg atctgctccg gccctggctc gtagaggtag tcgacaccac cttgctccgg
     1441 gccctcttcg aagtcgcgtg caatcggcag aaggcgttct gcgcgaacat gctgcgcgat
     1501 gatcgacttg aactcgttgt aaacgacgta gacgccgtcg atcttctctt cgatgaagtc
     1561 tttgatcagg ctgtctgcga tctcgtgggc cacgccaaga gacagcgcct gcgcaaccat
     1621 gacgttttgc cgaaccggga tgggacgccg cttgaagaag tcgccgccct tgcggccgat
     1681 ggtcaggacc gagacggatt cgaaggactg ttcgcggatg aagttctgcg cagcgcggat
     1741 gacgttggcg ttgaacgctc cacagagccc tcgatctgcc gtaacgacaa ccacgacgac
     1801 gttacgctcc ggctcacgcg actgcagcag cggatgcgcg ctgatgtcga cccgcgtgga
     1861 caccgaggag aggaccttgc ggagtcctgc ggcgtacggg cgggcagcaa acatccgctc
     1921 ctgcgcacgg cgtagtttgg ccgcggccac catcttcatg gccttggtga tctgctgcga
     1981 gttcttgacg ctgcggatcc ggcgccggat atcgatcgtg cttggcatga gtgattacgc
     2041 cgccagggcg ttggcgttgt ctttgacgta ttgcgccttg aattcctgaa tggcggtgcg
     2101 gagggcgcct tcgaggtcgg catcgaactt cgtcgcggtc tcgagacgat cgagcacgtc
     2161 gcccttctcg tcactcaggt actgatgcag cgccgtctcg aactgacgca ccgactcgac
     2221 cgcaagattg tcgagaaacc ctttgacgcc ggcgtaaacc gaggcggtct ggagagcgac
     2281 acgcatcggc tggtactgcc cctgtttgag gatctcgacc aggcggcgcc cgcgattgag
     2341 ctgcgactgc gtcgctttat cgagatcgga gccgaactgc gcgaaagcgg ccagctcgcg
     2401 atactgcgcg aggtcgagac gcagcgtgcc ggagacggag cgcatggagc gcacctgcgc
     2461 tgatccaccg acgcgcgaga ccgagatacc gacgttgatg gccggacgga cgccggagtg
     2521 gaagaggtcc gtctccagga agatctgtcc gtcggtaatc gagatgacgt tggtcggaat
     2581 gtaggcggaa acgtcgccgg cctgggtctc gatgatcggc agtgcggtca gtgatccgcc
     2641 gcccttctca tcggccatct tcgatgcgcg ctccagaagc cgcgagtgaa gatagaaaac
     2701 gtcgccggga tacgcttcgc ggccgggcgg acggcgaagc agcagcgaga tctcgcggta
     2761 ggccgcggcg tgcttggaga gatcgtcgta gatgatcagc gcatgctgac cgttgtagag
     2821 gaagtactcg gccatcgcgc agccgccgta cggcgccagg tattgcatcg gcgcgggatc
     2881 ggaggcggag gcgttcacga cgatggtgaa gtccatcgcc ccgttctcct cgagcgtgcg
     2941 aacgacctgt gcgacggtcg atttcttctg accgatggcg acgtagacgc agatgacgtc
     3001 tttgcctttc tgattgatga tcgtgtcgac ggcgacggcc gtctttccgg tttggcggtc
     3061 gccgatgatg agctcgcgct ggccgcggcc gatggggatc atggcgtcga cggccttaag
     3121 tccggtctgg agcggctctt taaccggctt gcggtcgacg acgcccggcg ccaggcgctc
     3181 gatcggatag aactcagtgg tgttgatcgg acccttgcca tcgatgggaa cgccgagggg
     3241 attgaccacg cggccgacga gagccgggcc gaccggaacg gacatgatct ttcccgtgcg
     3301 cttgaccacg tcgccttctt tgatcgctga ggcttcgccg aggaggacga cgccgacgtt
     3361 gtcttcctcg aggttgaggg ccagtccggc gatgtcgtga gggaactgaa ccagctcacc
     3421 ggccatgacg cggtcgaggc cgtacacgcg cgcgatgccg tcgccgacgg agacgaccgt
     3481 gccgacttca gagacatcga cgccgcggcc gattccgctt aattgctgcc gaataatctc
     3541 tgtgatttct tctgctttca aatcaacagc cataaagtct cccgaaaatg cttcgcgcta
     3601 aaagcatcac gctaatggca tcatgctaat gatgcgcgga atttctcgat cttccctgat
     3661 acggaagcgt cgtaaacctc gctcccgatc tttgccacga agcctccgat gagatccgga
     3721 tcgactgtga catcgatatc gacgtttgca ccgaccttgc gctcaagcat cgcgcgcagc
     3781 tcgctttgtt ccttcgccga aagctcgctg gcagagcgaa cctcggcggc cacggttcct
     3841 gttgcatcgc ggatcatcgc cgccagtccg gccacgatcg cggcgaggtc gttgatccgg
     3901 cggttctgaa tgaggacgtc gagcagccgc acggccagct cggacaggcc gagatgtccg
     3961 gcgatcgcag ccgtcacttt gtgcttcgaa tcgaagtcga tcccaggatt ggcgtacatc
     4021 tgctgcagat cgcgcgatga ttggcgaacc ttctcaaagg acgtcatttc gtcgcgaagc
     4081 gcggccgcct tttccggcga ctgaacggtg tccatgatgg cgcgtgcgta tggtctggcg
     4141 aatcgtcgga tcatgtacgt gccccttcca cttcccggac actctcttcg aagagccgct
     4201 cgcgatcgtg gtcggttacc ttttcacgga ggatctgctc ggcgcgctgc gtggccagct
     4261 cgccggcgaa ctcggttagt tcgtgacgtg catgtttgag ccggctctcc acttcgttgc
     4321 gggccgactg gaggatcttc tgcgcttcgg cttcggccgc ggcgatgagc tcgcgtttct
     4381 gccgctcgcc ttcaacctgc gcgcgctcct gaatgccgcg gacgtcggcc tcgatttgcg
     4441 tcagcctggc ctgaatgtcg ccggcgagtt gatcggcctt ggcgcgacgc gcgcgggcct
     4501 cttccgcatc cgtctgaatc tggacgcggc gcgcggcgag cgcgttcttg accgggccgc
     4561 cgatcagcca cccgagaacg ccgaagaaga ggagcatgtt gacgagcttc aggatccagc
     4621 caggaatccc gaaatacgtc ttcggcggtc ccagctcgcc tccgtgcgct tcgttcggat
     4681 gcgcctcctc gtgagaaacc ttctcggcac cttgcgcgac attgccggcg tcgttgccat
     4741 gcttctgcgc gaataggact gccggaacga gtagcagcgt aatgatcagg atcagacgcc
     4801 gcatcaaacc gctctcccca gaatccgttc cgctgccagt tgcgccaacg cttcggattc
     4861 gcgaacgatc ttcgcgcgcg cttcgacgac atcctgtttg agccgttcgt cggcttcgtt
     4921 gacgatcgcg tttgcttcgt tgtgcgtttt gtcgacgatg gaggtgcgat acgcggcagc
     4981 gtcgcggcgg aagtggtcgc gcacgtcggc agcggcgtgc cgtgcggcgt gaagctgatt
     5041 ctccatcagc gaggttgctt cgctgaagcg agccatcgac tgctcgtaga ggtcgttcgc
     5101 agagcgcact tcctcctcgc gcgcctcaat cacctgattg atgggccgta ggaagaacct
     5161 gctgacgatc agatagttca ggatgaaaat caccatgatc gcgagcagag agtaatcggg
     5221 cgtcagattg atttgcatag actgttttgg agccggaaaa accggcgcgt gagaaccgcg
     5281 ccgaaccccg aggcggcggt tgtataccac aagggaaaga acgttgtcac ctcgccggtt
     5341 gtgcgcggtg tgccggataa aacgtcaacg gaaacctata tcagcgtgct cgagcgcgcg
     5401 gatgatttct ttcgcggcgt cgccgcggcg caaccgcaaa gcctgcaatg cggacgcgga
     5461 tgttcgctct gttgctacgg catcttcgag atcggctcag gcgatgtccc gattctcgcg
     5521 gaagggctgg agaagctcca ccacaaacgc cgggcgatga tcatccgccg ggcgcgggag
     5581 atcgtttcgt catccgcgca tcctgatctt cgcgattgca cgccggccga gaaagcggct
     5641 ttcttcgaac ggaccgcgtc gacgccgtgt ccgaacctga acgagcgcgg cgagtgtttg
     5701 atgtacgagc accggccgct ggtctgccgc accttcggtc ttcctcttcg cgacggcgat
     5761 cgctacatcg gcgacatctg cgagctgaat ttccgcgatg cctcgcaacc ccagcgcgag
     5821 aaagccgcct gggacctgca gtgggaggac gccctcggcg ccgaggacga atacacgatc
     5881 cccgaggcga tcgtgctgat tgcgcgcatg cgcggatggt gaggtcttgc gcttcgcgcg
     5941 ttgtcggttg tcggttggtt gcgcttcgcg cgttgtgaag acggttttga caacgcggcg
     6001 aagccgcaat caactgacaa ccgacaacgc gcgaagcgca ataatcacgc tttggacgcg
     6061 atattcgtca ccgtcgcggg cttcttgtca cccatcacgc aattgccctg gaagatggcg
     6121 ccctcttcga tgatcagggc tggtgtatcg atgtcgctgt agaccttgcc gttgcggtgg
     6181 atctgcacgc tctgggcggc cacgatcttt ccgacgatcg ttccgctgat ggcgatgcgg
     6241 ccgacgtgga cgtcgccttc gatgtgcgcg ctctcgccga cgatcagctc gttcttcgac
     6301 atgatctttc cgttgaagcg gccgtcgata cgcatcgtgt cttcgaactc cagctcccca
     6361 tgaaacgacg agccgcggtc gagaaagcca ttcagttcac cactcttgtt tttcatatcg
     6421 ttccccatgc ccttcttcac tcccggcgtt cggattccgc gcagaatcag cacgaatccc
     6481 aacgcggcaa ccagcaccgc ggccaccaca acgacgagcg taccgctaac ggcgcctacc
     6541 catgagctca tcgacgactc ggatcaactc gtcttcgctg ccataacgaa tgtggatgac
     6601 gccaccgcgg cgcctgcgat cgatttcgac ttttgtacgc aaagtccttt gaagcttgtc
     6661 ttccgcgtcg cgcgtgaaga catccttttc cttctctttc ttttctaccg ctttgggaga
     6721 gctctccgac gcaagcatct ccgtctgccg gacgttgagg tttttgcgga ccacgcggtc
     6781 ggcgagttgc tcctgttttc gcgccgactc gatcgccagc aacgcccgcg cgtggcccat
     6841 cgaaagctga ccggatgcga ggagtttctt cactgggtcg ggcagcttga gaagccgcag
     6901 aaagttggcg acggtcgagc gttcctttcc gacgcgcttc gagatctcct cctgcgtcaa
     6961 ctggaactcg tcgtgaagct gagagtaggc aagcgcctct tcgatcgggt tgagatcctc
     7021 gcgctggatg ttttcgatga gagcgatttc aagtgcatcg ccctcctgca tcatctcttt
     7081 gatgagcgcg ggaacagtcg tcaggccggc cttctgcgca gcgcggaaac ggcgctcgcc
     7141 agcgatgagt ttgtatttgt cacccgcgcg cgtgacgacc agcggctgca cgatgccgtg
     7201 ctcgcggaca ctgcgcgcca gctcttcgat cgaggcctcg ttgaacgtct tgcgcggttg
     7261 gtaaggattc ggttgaatct gcgagatcgg aatctcggcc aagccctggg tagcatgagc
     7321 ctcgacaggc gcaggcggtt gctcctgccg ctgaggaatc agcgcgccga gaccgcgtcc
     7381 aagaactttc ctctggttca tcagattacg ttctccgcac gctgaatgaa ctcgcgagcc
     7441 aggctgacgt acgcctcgct gccgcgcgaa cggatatcgt aaagaataat cggtttgccg
     7501 aacgatggcg cttcgctgag gcgaatgttc cgcggaatga ccgtgcggta gaccttttcg
     7561 ccgaagtgcg tgcgcacctc ttccgcgacc tggcgcgtca gattcatccg gtcgtcgtac
     7621 atggtgagaa cgatgccttc gatctcgaga cctgggttga ccgctccctg cacgcgctcg
     7681 atcgtagcga gcagttggga gaccccctcg agcgcaaagt actccgtctg gagcggaacg
     7741 aggactgaat gggcggcggt aagtccattg acggtgagca aaccgaggga gggcgggagg
     7801 tcgatgaaga cgtagtcgta atcgcgcgcg acgggatcga gcacgcgctt gaggtagtac
     7861 tcgcggccga tggcctcccg cagctcgact tccgcggcga cgagatcgac cgaccccggc
     7921 gccacaaaca gcgtcgggat ctccgtcgga aggatgatgt ccgccaacgg catcttctcg
     7981 atgagaacgt cgtacatgga gcgctcggga tcgaggattc cgatcccgga tgtcgcgttt
     8041 gcctgcgggt cgaggtcgac cagaaggacg cgccgctcgc acgcggcgac cgatgcgccg
     8101 aggttgatcg cggttgtcgt tttgccgaca ccgccctttt ggttcgcaat cgcgaggatc
     8161 ttcgtcatgc tgggtgttcc acgtggaacg caagggtgac cagataattc gatgtgccac
     8221 gaggaagcgt gaaaacgtcc gtcttcgtga aacctcgtat gattggaacc tcaggcgacc
     8281 cttgaaaaag cgccacccgt ccctcgggag ccatccattt ccccagcgat ggcacccact
     8341 cctccggatt tccaaccgca cgcgatgtca cgagggaaaa ctgaaggtgg gaaggaaact
     8401 gcatcacagc gcgggccaat ctatcaccgt aaaccacagc gttcaattcg caggcgcgaa
     8461 cgatgtgttt cagaaaagcc cacttcttct tgtcggattc gacgagatga aagcgtgtcg
     8521 cgggcgaaac gatagccatc ggaatcgccg gaagtccccc gccgctaccg aagtcgagca
     8581 cctcgccctg cggcttcaaa atcccagccg cggcaaccgc ctcggcgata tggtcatcaa
     8641 gattcgccac cg
//
LOCUS       DATLVW010000011         4999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_83440, whole genome shotgun sequence.
ACCESSION   DATLVW010000011 DATLVW010000000
VERSION     DATLVW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83440"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1185
                     /locus_tag="VNN25_00330"
     CDS             109..1185
                     /locus_tag="VNN25_00330"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HXH89996.1"
                     /translation="MKLLGGEAKINELAYRTGAFVRKREVQSPADLLQMLMIWAVAER
                     SLRETVTLASEAGLAELSDVALLQRLKRAGPWIGGLLGEMLTQRQDVFAEGTRVRLID
                     ASAIARIGHRGTDLRVHLGLDLGLNCTDSIDITDGRGAESLDRFTFLPGEIVLADRGY
                     AHRKALARVSEAGAHFVVRLPWSSIPLEDHEGKPFDLFAALRSLPEATAGAFPVQFRV
                     SKSSVLCRLVAIRKTEAVAAKARQHAISESRRHGTVTDPRTLEAAGYFFVLTNLPDKY
                     TASKALELYRLRWQIEMKFKNLKSILHLGEVPARNGELFKVYVMAKLLVALVIENLIS
                     GESFSPWGYPIADTEPLATLAFPA"
     gene            complement(1360..2397)
                     /gene="rlmN"
                     /locus_tag="VNN25_00335"
     CDS             complement(1360..2397)
                     /gene="rlmN"
                     /locus_tag="VNN25_00335"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXH89997.1"
                     /translation="MNLYDLPSKQLEEALGAIVSPPFRIKQIVEWMHVHGVDTFDAMT
                     NLPKELRAALAARFTIAFPQVIERTTPAADGSQKYLFQLADGNRIEAVYMPMGERTSV
                     CLSSQAGCAVGCTFCVTGFFGAGRNLTPSEMIGQFFTIQHEHAVPMEMMNVVFMGMGE
                     PLLNFEHLVTTLDMLYRNIAPKRITVSTAGIIPGIDDLADLPRRPNLAVSINAPDRRR
                     REEIMPITKKYPLDDLIASLKRYPSEKGRETTIEYVLLSGYNDSHLDAVALAKLLHGL
                     RVKVNAIPFNEDPNLPAWMKRPKEGSIDRFADTLVRNGVAVTVRRSKGREIAAACGQL
                     RGKEVAKSRRL"
     gene            complement(2402..2746)
                     /locus_tag="VNN25_00340"
     CDS             complement(2402..2746)
                     /locus_tag="VNN25_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH89998.1"
                     /translation="MDDKIKKLLRELGNAINESISSSEDVNEHIQRIRDEGYNLYVVL
                     DATIGLNRIGEEEDAPKPVPSGMQVKSDREVQFRIDVNDLALLRALGIDPTRKVRGAR
                     RVEPLAVSSDEE"
     gene            complement(2826..3647)
                     /locus_tag="VNN25_00345"
     CDS             complement(2826..3647)
                     /locus_tag="VNN25_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HXH89999.1"
                     /translation="MRAEQTLHRPLVLGGKPVDLAISFAEGELRIDGAADAKNIEKNS
                     AELRRKLGRMFQLGIDTSEFVALSRGSQSHAWVSSSGFGRLLCGATLFEDVVKIIATT
                     NTMWKQTVRMTELLVGKCGRRTKSGRVAFPEPADVARFSSEELQEDCRLGYRAKSIHT
                     LASGIISGAIDLDAMSDPSQSTEELFASYRELPGIGPYGAAHLLAMDGRHDFIAVDTE
                     FRRFVRERYHGGRPVSDRTMLRRYAKWGRWKYLAYWSELWESVAAGLQPPDAEGQ"
     gene            complement(3779..3853)
                     /locus_tag="VNN25_00350"
     tRNA            complement(3779..3853)
                     /locus_tag="VNN25_00350"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3819..3821),aa:Cys,seq:gca)
     gene            3965..4040
                     /locus_tag="VNN25_00355"
     tRNA            3965..4040
                     /locus_tag="VNN25_00355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3998..4000,aa:Asn,seq:gtt)
BASE COUNT          952 a         1495 c         1548 g         1004 t
ORIGIN      
        1 gccgcgggcg agacgcccat aggcgctaac ttagcttagt gctacttttg atggatggaa
       61 ccaagggtgg atgagaggga gtgggttcag tatcaatggc cgtaccttgt gaagctgttg
      121 ggcggtgagg cgaagatcaa cgagctggcg tatcggacag gcgcgttcgt ccgaaagcgc
      181 gaggtccaga gtcctgcgga tctgttgcaa atgctcatga tctgggccgt ggcggaacgg
      241 tcgctgcgcg agacggtgac tttggcgtcc gaggccggac tggcggagct gagcgatgtg
      301 gcgctcctcc agcgtctgaa acgagctggc ccatggatcg gtgggctgct cggagagatg
      361 ttgacgcaac gtcaagacgt ctttgcagag ggtacccgtg ttcgtctgat cgatgcgtcg
      421 gcgattgcac gcattggtca tcgaggcacg gatcttcgtg tccatctggg gctggatctt
      481 ggactcaatt gcacggactc gatcgacatc accgacggtc gcggcgccga gagcctggac
      541 cggttcacct tcttgcccgg tgagatcgtg ttggctgacc gaggctacgc ccaccgcaaa
      601 gcgctggctc gtgtgagcga agctggagcg cacttcgtcg ttcggctgcc gtggtcgtct
      661 atccctctgg aagaccatga gggcaagccg tttgatttgt ttgcggctct gcgaagtctg
      721 cccgaggcca ctgccggcgc atttccggtg cagtttcgcg tatccaagag cagtgtcttg
      781 tgtcggttag ttgccattcg aaagacggaa gcagtggctg ctaaggcacg ccagcatgcc
      841 atctccgaga gtcgtcgcca cggtacggtg accgatcctc gtactctcga agcagcaggc
      901 tactttttcg tgctcaccaa cttgcctgac aagtacacgg ccagcaaggc gctggagttg
      961 taccggctgc gttggcagat cgaaatgaag ttcaaaaacc tcaaatcgat actgcatctc
     1021 ggcgaagtgc cggcgcgtaa tggcgagctg ttcaaggtgt atgtcatggc caaactgctc
     1081 gtcgcactgg tcatcgagaa tctgatttcc ggagagtctt tttccccctg gggataccca
     1141 atcgcggaca ccgagccatt ggcgactctc gcgtttcctg cttgaccttc tgatcgacgc
     1201 gatcaaaccg catctcacct tcaagcgggc ccgtcgtcgc aaagcggcca gacgccgaca
     1261 cttctcgaac ccgccacgaa agcggaagaa tcagcgcatc gccgccatca tcctctgcgg
     1321 aggtctaagt tagcgcctat gggcgagacg cccgcactcc tataggcggc ggctcttggc
     1381 gacttctttc ccgcgcaact gcccgcatgc cgcggcgatc tcgcgccctt tcgaccggcg
     1441 cacggtaacc gcgacgccgt tgcgaacgag cgtgtcggcg aagcgatcga tcgagccttc
     1501 cttgggacgc ttcatccagg cggggagatt cggatcctcg ttgaacggga tggcgttcac
     1561 tttcacgcgg agtccgtgga ggagcttcgc gagtgccacc gcatcgagat gcgaatcgtt
     1621 gtagcccgac agcagaacgt actcgatggt ggtctcgcgt cccttctcgc tcgggtagcg
     1681 cttcagcgaa gcgatcagat catcgagcgg atacttcttc gtgatcggca tgatctcttc
     1741 gcggcgacgg cggtcgggcg cattgatcga gacggcgagg ttgggccggc gcggcaggtc
     1801 cgcaagatcg tcgatgcctg gaatgatgcc cgccgtcgac accgtgatcc gctttggcgc
     1861 gatgttgcgg tagagcatgt cgagcgtcgt cacgagatgt tcgaagttca gcaacggctc
     1921 gcccatcccc atgaagacga cgttcatcat ctccatcggc accgcgtgtt catgctggat
     1981 cgtgaagaac tgtccgatca tctccgacgg cgtcaggttg cggcccgcac cgaagaaacc
     2041 ggtcacgcag aacgtgcagc cgaccgcgca gcctgcctgc gatgagaggc acacgctggt
     2101 gcgttcgccc atcggcatgt acaccgcttc aatgcgattg ccgtcggcga gctggaagag
     2161 atacttctgg ctgccatccg ccgcgggagt ggtccgctcg atcacttgcg ggaaggcgat
     2221 cgtgaatcgc gcagcgagcg cggcacgcaa ttccttcggc aggttcgtca tagcgtcgaa
     2281 cgtatcgaca ccgtgaacgt gcatccactc cacgatctgc ttgatgcgga agggagggga
     2341 gacgatcgca ccgagtgcct cttcgagctg cttcgaaggc agatcgtaga ggttcacgcg
     2401 ctcactcttc gtcactgctg accgcgagcg gttctacgcg ccgtgcgcct cgtactttgc
     2461 gggtgggatc gatgccgagg gcgcggagga gggccagatc gttcacgtcg atgcggaatt
     2521 gaacctcgcg gtcgctcttg acctgcatgc ccgacggcac gggcttcggt gcatcttctt
     2581 cctcgccgat gcggttgagg ccaatcgtgg catcgagcac cacgtagagg ttgtagccct
     2641 catcgcgaat gcgctggata tgctcgttca cgtcttcaga gctggagatc gactcgttga
     2701 tcgcgttccc gagctcgcgc aaaagcttct tgattttgtc gtccaaggtt ccgttccggc
     2761 tgcaaggaag tgtagtacgc catcggcgaa ggtcaatatt ccgaagcggc gcctgggagc
     2821 gtatgctatt ggccctctgc gtccggcggc tgcaaaccag cagccaccga ctcccacaat
     2881 tccgaccagt aggccaggta cttccagcgg ccccatttcg cgtagcgccg cagcatcgtg
     2941 cggtcggaaa cggggcgccc gccgtgatag cgctcgcgaa cgaatcttcg gaattccgta
     3001 tcgacagcga tgaaatcgtg gcggccgtcc atcgccagaa ggtgcgcagc gccgtacgga
     3061 ccgatgcccg gcaactcccg ataactggcg aacaactcct cggtcgattg cgacggatcg
     3121 ctcatcgcgt ccagatcgat tgcaccgctg atgatcccgc tggcgagcgt gtgaatcgac
     3181 ttcgcccgat accccagccg gcaatcttcc tgaagctcct cggacgaaaa gcgcgcaacg
     3241 tccgccggct ccggaaaagc gacccgtccc gacttcgtgc ggcggccgca cttcccgacc
     3301 agcagctccg tcattctcac cgtctgcttc cacatcgtgt tcgtcgtggc gatgatcttg
     3361 acgacatctt cgaacaacgt cgcaccgcaa agcagccgtc cgaaccctga cgacgagacc
     3421 cacgcgtgcg attgcgaccc tcgggagagc gcaacgaact cgctggtgtc gatccctagc
     3481 tgaaacatgc gcccgagctt gcggcgcaac tcagcgctgt tcttctcaat gttcttcgcg
     3541 tcggccgccc cgtcgatccg cagctcgccc tcggcgaacg agatcgccag atcgaccggt
     3601 ttgccgccca gaaccagcgg ccggtgcagc gtctgctccg cgcgcaccca tcgaaacggc
     3661 gccagcagat accagccatg ggagacgacg gtcgaaccga agtgaaaatt ggcaggagtc
     3721 ggaagggagg tggaggtggg aacagacgcg ggtgaggaaa gggatgtggg cgaggaaatg
     3781 gaggcgacgg ccgggatcga accggcgaat ggaggttttg cagacctctg ccttaccact
     3841 tggctacgtc gcccatcgga ggaaagttcg cgagtgtagt gaaccgaacg tagggcgtca
     3901 atattcgtag gttgacacgc atcgcctctc tcggccacca tgccgcccct tcgcagcaag
     3961 atactgtggt gtagctcagt tggtagagcg ccggattgtt aatccggtgg tcggaggttc
     4021 gagtcctccc gccacagcca atcttcactt tcgggcggaa gcgggccatt tccgaccagc
     4081 acatcgaacg gcgaatgcaa agtggatgag agacttcggc cgtttttcac ttcgccaacc
     4141 gtgaggttcg agaagaggat tttgcagagt gcgttctttt cttcggcggg ctggctaagg
     4201 tacagagctg gcgcagcttg gagcagttcg agaggtgcag cccccgctcg cagaaaggtc
     4261 gtgcgatcga cggcggggcg gtcgagcgcg acggcgatac tcaacgcatc gatctcggcc
     4321 tgccagtcag cgacgaaggc gcgataggtt gcctcatcca tcgcaccgtc tgatttctct
     4381 tcccaggctt ttcgcatccg ggcacgcagg tgcgcgatgc gctgctcgcg ctggcgctga
     4441 tcgttcccgt cccgctcgat agcggcgtcg taccacgacg cgagttccgc ctgaatccac
     4501 gatgacacgg cctcgtcgat ctggagcgac tggagaaccg aaagcacttc gctatcgaag
     4561 acgcgttcgg ggtagaacgt gcggcagcct aaccgtccgc cgcagctgta gtagatgaag
     4621 acgtgttcgg ctttcgttgt cttcttccgt ttccgatcgc cggagagcat gccgccacag
     4681 cgcgcacagc ggagcagacc ggagaaggcg aagatgaact tgttcttctt gggtctattc
     4741 cgattgccga ggacgcgctg cacgcgctcg aagagcgatc ggctgatgag gggcggatac
     4801 tttccttcgt aggtgacgcc gccccaatca aacacgccga agtagatcgt gttcgtcaac
     4861 acgttgacga tctggctctt gcccacgcgc ttcccagtac gcgagtgcac gccgatctcg
     4921 aacgcgaagc atgccagatc gcgcaggctc atctcgccct tggccgcttc ttcgaagagg
     4981 cggcgaatga gtggtccaa
//
LOCUS       DATLVW010000012         3952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_331742, whole genome shotgun sequence.
ACCESSION   DATLVW010000012 DATLVW010000000
VERSION     DATLVW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331742"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VNN25_00360"
     CDS             complement(<1..802)
                     /locus_tag="VNN25_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90000.1"
                     /translation="MDSAAVNRQSRNDEVAPAPTPDPRPPTPATIIIALYALLAAIII
                     PVFPHFPSPNELTRWVVVASVVDDHSLEVSRAAQVLGPQFEDLAVIDGRQYSNKAPGA
                     ALVASPGYLLARPFAGPPSANNLRPVLTAMRWFGATLPLLFAALAFAHYARTRGGDPA
                     LAIAAMLFATPLFAYGLLIFSHALTAAALFGAWPLLYLRDRGGVVAGVLIGVAVASEY
                     PMLVPALVLIGGLLAQRAWGRLARVVAGGAPFALMLAIYQKLAFGSILA"
     gene            complement(812..1504)
                     /locus_tag="VNN25_00365"
     CDS             complement(812..1504)
                     /locus_tag="VNN25_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HXH90001.1"
                     /translation="MKKRFPYYPIYIDIEDHAVLIVGGGVVCARKAETMMRYGGRVTI
                     VSPEITDEIAAWERDGALTVHRKRYEEADLDGASIVIASTDDPCVNARVARDCRRRKV
                     PVNVVDVTHLCEFIVPAIIEKGSIQIAISTGGKSPALARTLKEDLQRTIGPEYAEVND
                     LLGTLRKEAKRILPTDTDRKRFFDGIIAAGVLEMLREGRKCDAYQAIVSACDAEGVPV
                     SEELQERLASAM"
     gene            complement(1586..2866)
                     /gene="glyA"
                     /locus_tag="VNN25_00370"
     CDS             complement(1586..2866)
                     /gene="glyA"
                     /locus_tag="VNN25_00370"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXH90002.1"
                     /translation="MTTIEEQRAATGHDFLQRTLSDVDPKIAAAIALETERQNDTVEL
                     IASENHVSRAVLTAMGSVFTNKYAEGYPGKRYYGGCGPTDIVETIAIDRAKQLFGAEH
                     ANVQPHSGSQANMAVYFTVLKPGDTIMGMDLSHGGHLTHGHPLSYSGRDFNVVAYHVR
                     QEDELIDYDEMERLALEHKPRLVICGASAYSRIIDFKRIREICDKSGSLMMADMAHIA
                     GLVAAGLHPSPVPYADFVTTTTHKTLRGPRAGLVLCKAAYAKDLDRSLFPGIQGGPLV
                     HIIAAKAVAFEEALQPEFKEYQAQIIRNARALAQAVAGTGFRIVSGGTDNHLFMADVF
                     SKGITGKDGQNFLEAANITVNKNTIPFDTQKPMVASGIRIGTPAVTTRGLREPEMQRI
                     AELIARVLDSKGDAGVIVEVKKGVKELCDRFPIY"
     gene            2922..>3952
                     /locus_tag="VNN25_00375"
     CDS             2922..>3952
                     /locus_tag="VNN25_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90003.1"
                     /translation="MTVRPRLLLLLLLAVLGVLLASFGFFFFRDNFATHYPIKVLSAA
                     VFRAGEIPWWNFADGGGQPLAGNPNTLTFYPDNFLYLLLPAHFAFNLHFLLHLALGWL
                     AMKSLTRSNFGATLWVLSGVTISALSFYNLVTAIALVPLAFLGAERRSAMKLGLAFGL
                     LALAGEPVTIAATAIAVLILFTRRREDAKETIPIFASSRLRVSIVSLGIAVVIAAPQL
                     IAYFEIASEVERAHRYSAHTVLSASFDPRRLLELIAGPFLSIDTPHLFPSLMIGLIVI
                     PALFRRSRYTLIAAVMFFFALGSFNPIVRWLVESIDVLRAGRFPEKFALVLCAALVVL
                     AAEYFGRSAT"
BASE COUNT          656 a         1268 c         1233 g          795 t
ORIGIN      
        1 atgccaggat gctgccgaac gcgagcttct ggtagatcgc cagcatcagc gcgaacggcg
       61 cgccgcccgc gacgacgcgc gcgagccttc cccacgcgcg ctgcgccagc agtcctccga
      121 tcaggaccag cgcggggacg agcattggat actcggacgc gacagcgacg ccgatcagca
      181 cgccggcaac gacgcctccg cgatcgcgca ggtagagcag aggccatgcg ccgaagaggg
      241 ccgcggcggt aagcgcgtgc gagaaaataa ggagtccgta cgcgaaaagc ggcgtggcga
      301 aaagcatcgc cgcgatcgca agcgccggat cgccgccgcg tgtgcgtgcg taatgcgcga
      361 aagcgagggc ggcgaacaga aggggtagcg tcgcaccgaa ccaacgcatc gcggtcagca
      421 ccgggcgcag attgttcgcc gacggcggac cggcgaatgg ccgcgcaagc aggtagccgg
      481 gcgaggccac cagcgccgca cccggtgcct tgttcgagta ttgccgtccg tcgataacgg
      541 cgagatcttc gaactgcggt ccaagaacct gcgctgcgcg cgagacctcg agcgagtgat
      601 cgtcgacgac cgacgcgacc accacccagc gggtcagttc attcggcgac gggaaatgcg
      661 gaaagacagg aatgatgatc gccgcgagca gggcatagag cgcgatgatg attgtggcgg
      721 gagtcggggg tcgggggtcc ggggtcggag cgggtgcaac ctcatcgttc cgagactgtc
      781 gattgaccgc tgccgaatcc attggtgcag cctacatcgc cgacgccagc cgctcctgca
      841 attcctcgct caccggcact ccctcggcat cgcaagcgct gacgatcgcc tggtacgcat
      901 cgcactttcg tccctcgcgt agcatttcga gaacgcctgc ggcgatgatc ccgtcgaaga
      961 accgcttgcg gtcggtgtcc gtcggaagga tgcgcttcgc ttctttccgc agcgtgccga
     1021 gcaaatcatt cacctcggcg tactccggtc cgatcgtccg ctgcagatct tccttgagcg
     1081 tccgcgcgag cgccggtgat ttcccaccgg tcgagatggc gatctggatc gatccttttt
     1141 cgatgatcgc cgggacgatg aactcgcaga ggtgtgtgac gtcgaccacg ttaacgggca
     1201 ccttgcggcg ccggcagtcg cgggcgacac gcgcgttgac gcagggatcg tcggtggaag
     1261 cgatgacgat cgacgcgccg tcgaggtcag cctcttcgta ccgcttgcga tgcacggtca
     1321 gcgcgccatc gcgctcccac gccgcaatct cgtccgtgat ctccggactg acgatcgtca
     1381 cgcggccgcc gtaacgcatc atcgtctccg ccttccgcgc gcagacaact ccgccgccga
     1441 cgatcaagac cgcgtgatcc tcgatgtcga tgtagatcgg gtagtagggg aagcgttttt
     1501 tcaatgttcg tagtgctcgc gggagtcggg tgtcgggagt cggagcctca gaatctccga
     1561 ctcctgacac ccgatcagcg actccctagt agatcgggaa ccggtcgcac agctccttca
     1621 cacctttctt gacctcgacg atcacgcccg cgtcgccctt ggaatcgagg acgcgcgcga
     1681 tcagttcggc gatgcgctgc atttccggtt cgcgcaaacc gcgcgtggtt actgccggcg
     1741 ttccgatgcg gattccggac gcgaccatcg gcttctgggt gtcgaaagga atcgtgttct
     1801 tgttgaccgt gatgttcgcc gcctccagaa agttctggcc gtccttgccg gtgatcccct
     1861 tcgagaacac gtcggccatg aaaaggtgat tgtcggtgcc gccgctgacg atccggaatc
     1921 cggtgccggc cacggcctgc gccagcgccc gtgcgttgcg aatgatctgc gcctgatact
     1981 ccttgaactc cggctgcagc gcttcctcga acgccaccgc tttcgccgcg atgatgtgca
     2041 cgagcgggcc gccctgaatg cccggaaaga gcgagcgatc gagatccttc gcgtaggcgg
     2101 ctttgcagag cacgagtccc gcgcgggggc cccgcagcgt cttgtgcgtc gtcgtcgtga
     2161 cgaagtcggc gtagggaacg ggggaagggt gcaggccggc ggcgacgagg cccgcaatgt
     2221 gcgccatgtc ggccatcatc agcgatccac tcttgtcgca gatctcgcgg atgcgcttga
     2281 aatcgatgat gcgtgagtaa gccgaggcgc cacagatgac cagccgcggt ttgtgctcca
     2341 gcgcgagacg ctccatctcg tcgtagtcga tcaactcgtc ttcctggcgc acgtgatacg
     2401 cgacgacgtt gaagtcgcgg ccggagtacg aaagcggatg tccgtgcgtg agatgaccgc
     2461 cgtgcgaaag gtccatcccc atgatcgtgt cgcccggttt gaggacggtg aagtagaccg
     2521 ccatgttcgc ctgggaaccg gagtggggct ggacgttggc gtgctcggcg ccgaaaagct
     2581 gcttggcccg gtcgatggcg atggtttcga cgatgtctgt cggaccgcat ccgccgtagt
     2641 acctcttccc cggatagccc tcggcgtact tattcgtgaa gaccgagccc atcgccgtga
     2701 gcacggcgcg cgaaacgtgg ttttcggagg cgatcagctc caccgtgtcg ttctggcgct
     2761 cggtctccag cgcgatcgct gcagcaatct tcgggtcgac atcgctgagc gtccgctgca
     2821 ggaaatcgtg gccggtagcg gcgcgctgtt cttcgatcgt ggtcatcgtc atctccttcg
     2881 aggagggtcg gcgggcgatc ttactcgata atcagcgccc gatgactgtg cgcccgcggc
     2941 tccttttgct ccttttgctc gctgttctcg gcgtgctgct cgcttcgttc ggcttcttct
     3001 tttttcgcga caacttcgcg acgcactatc cgatcaaggt gctctccgct gccgtgtttc
     3061 gggccggcga gattccgtgg tggaacttcg ccgatggtgg cgggcagccg ctggccggca
     3121 acccgaacac gctcacgttt tatcccgaca acttcctcta tctcctgctg cctgcacact
     3181 tcgcgttcaa tcttcacttc ctccttcacc tcgccctcgg ctggctggcg atgaagagcc
     3241 tcacgcgctc gaacttcgga gcgacgctgt gggtgctgtc cggcgtcacg atctcggcgc
     3301 tctcgttcta caacctcgtc accgcgatcg cactcgttcc tcttgccttc ctcggcgccg
     3361 aacgccggag cgcgatgaag ctcggtctcg cattcgggtt gctggcgctg gcaggggagc
     3421 cggtgacgat tgcggcaacg gcaattgctg ttctgatcct tttcacgcga agacgcgaag
     3481 acgcgaagga aacaatccct atcttcgcgt cttcgcgtct tcgtgtgagc atcgtttccc
     3541 tcggaatcgc tgtcgtcatt gctgcccccc aactcattgc atacttcgaa attgcgtccg
     3601 aggtggaacg cgcgcatcgt tactcggcgc acaccgtgct cagcgcatca ttcgatcccc
     3661 ggagattgct ggagctgatc gccggtccgt tcctctcgat cgacacgcca catctcttcc
     3721 cgtcgctgat gattggcttg atcgtgattc ctgcgctctt ccgccgttcg cgctacacgc
     3781 tcatcgccgc ggtcatgttc ttcttcgcgc tcggctcgtt caatcccatc gtgcgatggc
     3841 tggtcgagtc gatcgacgtg ctgcgcgccg gccggttccc ggagaaattc gcactcgtcc
     3901 tctgcgcggc cctggttgtt ctcgccgcgg agtacttcgg aagatcggcc ac
//
LOCUS       DATLVW010000013         3357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_248637, whole genome shotgun sequence.
ACCESSION   DATLVW010000013 DATLVW010000000
VERSION     DATLVW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248637"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VNN25_00380"
     CDS             <1..496
                     /locus_tag="VNN25_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90004.1"
                     /translation="SFNVSVSLDDLVLARVRQFFCDRAPVSLRDAAMIARSDPAWVLT
                     QIENPERASAGDAIPWPEVAEIVQRTWTPADLDAAVGTVEGFPRLLRMSLVHWRLPAY
                     LVIALEHIVVEMRADSPEGETLTVESYVARLLDLFMDMDRVQRLCSDPAFREAFEFPD
                     REES"
     gene            501..890
                     /locus_tag="VNN25_00385"
     CDS             501..890
                     /locus_tag="VNN25_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90005.1"
                     /translation="MAKITSDKTKFVPLAGDLSQGAAINKTSASERVLIPESAIDVDD
                     IVFRAREDIPAVFVQRGDLLIVERRKPDHAATGELVVVLIGDRSFIGHWWTKRGERAL
                     VNDGLDLVTEDKKMRIVGAITAILRWR"
     gene            complement(949..1230)
                     /locus_tag="VNN25_00390"
     CDS             complement(949..1230)
                     /locus_tag="VNN25_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90006.1"
                     /translation="MARFDPSMARFHPSMARFDPSMVRFHPSMARFHPSMARFDPSTA
                     RFDPLTARSRPSTVLFSHRRFFSAIDGSFRPSMALFSHRRLFSAIDGVF"
     gene            complement(1513..2787)
                     /locus_tag="VNN25_00395"
     CDS             complement(1513..2787)
                     /locus_tag="VNN25_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH90007.1"
                     /translation="MKSKLLLTFAVALVVLTPALRANWNQDATVTIVATDDRDDDRAD
                     RDRDRAQQKADMEEDLYDSATDALDDHNWRSAASKFQRVAEMHLAHADAALYWLAYAE
                     GKMGARSEALDTLLKLQKSYPKSKWVEDGKTLEVEIRQSAGQQIEPEHVVDEDVKLMA
                     INGLMNSDPERGIPILEGILNGHQPIKMKERALFVLSQSGSPKAFEIVVRTAKSGPPD
                     LRERAVRTLGILGGERSRSVLTDIYTSTSDIAVKKSILKSYMIAGDRTRLLALAKAET
                     NTELRADAVLQLGVIGARNELADLYSTETAVPVRKKILQAMFIGGNSEKLAEIARNEK
                     VLELKTTAIRNLGLLGGGRSGDLLLSLYNSDTRPEVRNAVIESLFIQNNGHALVNLAR
                     SEKDPEMKRRIIEKLSVMRSKEATDYLMEFLK"
     gene            complement(2784..>3357)
                     /locus_tag="VNN25_00400"
     CDS             complement(2784..>3357)
                     /locus_tag="VNN25_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90008.1"
                     /translation="RRRSRVWVTISAIAAALALAFIGGMWWHARTRQTSPVVIARSTP
                     SPAAAAQTASNSSSAVSRDRLLLVVVSDHLDSSERMLLELSNADAKHPLDVTSESRRA
                     GELVASNRIYRQTAARRGETRIASLLSDLEPVLLELSHAGSTLSPDEIASLQKRIDSK
                     GLLFKVRVVSAQVSGQEAVTKAPPKGMDSL"
BASE COUNT          666 a          985 c         1056 g          650 t
ORIGIN      
        1 ctccttcaat gtgagcgtgt ctcttgacga cctcgtcctt gcgcgagtgc gccagttctt
       61 ttgcgatcgt gcgcctgtaa gcctccgtga tgccgcgatg atcgcgcgct ccgatccagc
      121 gtgggtgctc acacaaatcg agaatccgga gcgcgcttcc gccggtgacg cgatcccatg
      181 gccggaggtt gccgagatcg tccagcggac atggacgccc gccgatctcg atgccgccgt
      241 gggcacggtc gaagggtttc caaggctgct ccgcatgtcg ctcgttcatt ggcgtctccc
      301 tgcatacctg gtgatcgcgc tggagcacat cgtcgtcgag atgcgcgcgg actcccctga
      361 aggggagacg ctcacggtcg agtcctacgt cgcgcggctg ctggaccttt tcatggacat
      421 ggacagggtg cagcggctct gcagcgaccc tgcatttcgc gaagcattcg agttcccaga
      481 ccgcgaggag agctgaggcg atggcgaaaa taacgagcga caagacgaag ttcgtgcccc
      541 ttgccggaga tctgtcccaa ggtgctgcaa tcaataaaac ctcggcgagc gaacgggtac
      601 tcattcccga aagcgcgatc gacgttgatg acatcgtctt ccgcgcgcga gaggacatcc
      661 cagcagtctt cgttcagcgc ggagatctcc tgatcgtgga gcgccgaaaa cccgaccacg
      721 cggccacggg tgagctagtc gtcgtcctga tcggtgaccg ctccttcata ggccactggt
      781 ggaccaaacg cggggagcgc gcactcgtca acgatggatt ggacctggtc actgaggata
      841 agaagatgcg gatcgtcggc gccatcacag ctattcttcg ctggcgctga agcaatgatg
      901 tcgtgcttgt aacaagacta ctaccaacca agaagcaccg ccgatggttc aaaatacacc
      961 atcgatggct gaaaagagcc gccgatggct gaaaagagcc atcgatggcc gaaaagagcc
     1021 gtcgatggct gaaaagaacc gtcgatggct gaaaagaacc gtcgatggcc gagaacgagc
     1081 cgtcaatgga tcaaaacgag ccgtcgatgg atcaaaacga gccatcgatg gatgaaaacg
     1141 agccatcgat ggatgaaaac gaaccatcga tggatcaaaa cgagccatcg atggatgaaa
     1201 acgagccatc gacggatcaa aacgagccat cgatggccga aaagaaccgt cgatggatca
     1261 aagcgcaccg tcgatggatc aaaacgaagc gtcgatgatg tgaaactcag gtacgaacgc
     1321 gctaaccacg gctccgacgg ctcacaacgc acccgttcag gagaaactca tcgcttcacg
     1381 cgacgcaaag gaatcgctga acgagtgtgg agagatacag gctggaaatg cacgaaggat
     1441 gagacgcgtc atttgccagt ttcccacgcg ttctcacaca ccccactccc tacaccccac
     1501 ccccccagcg cactacttca aaaactccat cagatagtcc gtcgcctctt tcgagcgcat
     1561 caccgacagc ttctcgatga tcctgcgctt catctcgggg tccttctcgc tgcgtgcgag
     1621 gttgaccagg gcgtgcccgt tgttctggat gaagagtgat tcgatgacgg cgttgcgtac
     1681 ttcggggcgg gtgtcggagt tgtagagcga gagaaggagg tcgccggagc ggccgccgcc
     1741 gaggaggccg aggttgcgga tggcggtggt tttgagctcg agaaccttct cattgcgcgc
     1801 gatctccgcc agcttctcgg agttgccgcc gatgaacatc gcctgcagga tctttttgcg
     1861 gaccggcacc gccgtctcgg tgctgtacag atcggccagc tcgttccggg cgccaatgac
     1921 gccgagttgc agaacggcat cggcgcgcag ctcggtgttc gtctcggctt tggcaagagc
     1981 gagcagacgc gtgcggtcgc cggcgatcat gtacgacttg aggatcgact tcttcacagc
     2041 gatgtcgctc gtcgacgtgt agatgtcggt cagtacgctg cggctgcgct ccccgccgag
     2101 gatgccgaga gtgcggacgg cgcgctcgcg aagatcgggc ggacccgatt tcgccgtgcg
     2161 cacgacgatc tcgaatgctt tcggcgaacc cgattgactg agcacgaaga gagcgcgctc
     2221 tttcattttg atcggctgat gaccgttcag gatgccctcg aggatgggga tgccacgctc
     2281 ggggtcggag ttcatcaggc cgttgatggc catcagcttc acgtcttcgt cgacgacgtg
     2341 ctccggctcg atctgctggc cggccgactg ccggatctcg acttcgagcg tcttcccgtc
     2401 ctcgacccac ttcgacttcg gatacgactt ctggagcttg aggagtgtgt cgagcgcttc
     2461 cgaccgtgcg cccatcttcc cttcggcgta cgcgagccag tagagcgcgg catcggcgtg
     2521 cgccaggtgc atctcggcga cgcgctgaaa cttcgaggcg gcgctccgcc agttgtgatc
     2581 gtcgagcgcg tcggtggcgg agtcgtagag atcttcctcc atgtccgctt tctgttgcgc
     2641 gcggtcgcga tcgcggtcag cgcggtcatc gtctcgatcg tccgtagcga cgattgttac
     2701 ggtggcatct tgattccagt tggcgcgcag ggccggcgtg agcacgacca gcgcgacagc
     2761 aaatgtgaga agcagtttcg atttcatagg gagtccattc ctttcggcgg ggctttggtg
     2821 acggcttcct ggccgctcac ctgtgcgctg acgacgcgca ctttgaagag gaggcctttc
     2881 gagtcgatgc gcttctggag cgatgcgatc tcgtcaggcg agagagtcga gccggcatgc
     2941 gacagctcga ggagcaccgg ctcgaggtcc gacagcaacg atgcgatacg cgtctcgcct
     3001 cgccgtgcgg ccgtttgccg gtagattcga ttcgatgcga cgagctcacc ggcccgtctg
     3061 ctttccgagg tgacgtcgag cggatgtttg gcgtcggcgt tcgacagttc gagcaacatc
     3121 cgctcggagc tgtcgaggtg atcgctgacg acgacaagca ggagccggtc gcgcgaaaca
     3181 gcggaagagg agttgcttgc cgtctgcgct gcggccgcag gtgacggagt gctcctggca
     3241 atgacgacgg gagacgtttg ccgcgtccgg gcgtgccacc acatgccgcc gatgaaggcg
     3301 agcgccagag ccgctgcgat tgcgctgata gttacccaga cgcgcgagcg gcgccgt
//
LOCUS       DATLVW010000014         2514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_331869, whole genome shotgun sequence.
ACCESSION   DATLVW010000014 DATLVW010000000
VERSION     DATLVW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331869"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..>2514
                     /locus_tag="VNN25_00405"
     CDS             90..>2514
                     /locus_tag="VNN25_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXH90009.1"
                     /translation="MFEMINPPSGTANVVVTLAAGGGVNMCAGAISFNNVHQTTPRRA
                     TAVANSQSQPGGKGTNPIVTVTSVARDAVVDAAISSTGTTTMTAASPQLVEWNIDSNA
                     GAGGGGETNLGCSILWATSTSTTMSWTLSVQDKWAIIATSLIPAVPPTVVRLREVTVT
                     RDNRRILISWQTSFELDNLGFNIYRDVSGQRTKINKHLIAGTAFQSKQQDAEAGHVYR
                     FGDSLDEGIFAQYWLEDVDTAGRHTMHGPVSPVGGVIDSEPNTTPLSGLGSGGEVFAS
                     PDGVGAARASVQEPASATQYAQQRDLASDNGLKIFVSQEGWYRLTRAAMTAAGFDPGA
                     DPRAISLYMLGAEQSIVVDDGDDGTLDANDTIEFYGYPLDSVSTGARTYWLRPKKGNE
                     PRVAISKIKGGDPITGSASYVYERVERSGFFAALTTNGEADNFFGEVIGNWTATEPLK
                     VGNLDPAYSGNATLDLVIQGGSLMEHRIAVAINGHDLSTVVLQRTEQPTFTFQLPQSW
                     LVSGSNDVTLTSLNGDDDISGMAKSRLTYQHLLRADNGALDVSLPGGRSVTVGGFSVK
                     TVRAFDITDPRHPTELQATVAADPQGGFAATFSTPADGMRTVLAIDSSRLLTPGELTA
                     NTPSSWSATTTSAKNSASFFIISHRSFLGAAATLKALRDNQGISTAVVDVDDLYDEFN
                     FGIRSPEAIRSFFRAAAGWKHPPSAALLLGDASMDPRNYLGVASVDFVPTKLVPTFYM
                     KTASDDWFTDFNGDGIADIPVGRIPVRTPQEASLVIGKITSRGTPAGPWSKTALFVAD
                     GPGGFDFPGV"
BASE COUNT          484 a          793 c          773 g          464 t
ORIGIN      
        1 gaagggcgat caactctgtt acctatggag gcacggcact caccgtgttc gcgtcagagt
       61 tcacgccagg ttcgaaagac ctggtggcga tgttcgagat gatcaatccg ccctcgggaa
      121 cggcgaacgt ggtcgtcacc ctggcagcgg gcggtggcgt caacatgtgt gccggggcga
      181 tctccttcaa caatgtccat caaaccactc cgcggcgcgc cacggcggtg gctaattccc
      241 aaagccagcc tggcggcaaa gggacgaatc cgatcgtcac agtgaccagc gtcgctcggg
      301 acgcggtcgt cgatgccgca atcagctcca ccggcacgac cacgatgaca gcggccagcc
      361 cgcagctcgt tgagtggaac atcgactcga acgccggtgc tggcggtggc ggagaaacga
      421 atctcggctg cagcatctta tgggccacgt cgacatcgac aacgatgtcg tggacgctca
      481 gcgtgcagga caagtgggcg attatcgcca cctcactaat ccctgccgtt ccgcccaccg
      541 tcgttcgtct gcgcgaggtc acggtgacgc gggacaacag gcgaatcctc atttcatggc
      601 aaacgagctt cgagctcgac aatcttggct tcaacattta tcgcgacgtc tccggacagc
      661 ggacgaagat caataagcac ctaatcgccg gaacggcgtt tcaatcgaag cagcaagacg
      721 cagaggcggg ccacgtctac cgcttcggcg acagcctgga tgagggcata ttcgcgcagt
      781 attggctgga ggacgtcgat acggccggcc ggcacacgat gcatgggccg gtctcaccgg
      841 tgggcggtgt catcgacagc gaaccgaata cgacgccgct ttcgggcctg ggctctggag
      901 gcgaggtatt cgcatccccc gatggcgtcg gtgctgctcg cgcctctgtg caggagccgg
      961 cgtccgcgac acagtacgcg cagcaacgcg atctcgcttc cgataacgga ctgaaaatct
     1021 tcgtcagcca ggaagggtgg tatcgcctca cgcgcgcggc gatgacggct gccggttttg
     1081 atccgggcgc cgatccgcgc gcgatttccc tctacatgct cggggccgaa caatcgatcg
     1141 tcgtggatga cggcgacgac ggcacgctcg acgcgaacga caccatcgag ttttacgggt
     1201 acccgctcga ttcggtctcc actggagcac gcacgtactg gctccgcccg aagaagggca
     1261 atgaaccgcg ggtcgcgata tcgaagatca agggtggcga tccgattacc ggcagcgctt
     1321 cgtacgtcta cgagcgggtt gagcgaagcg gcttttttgc ggcgctgacg acgaatggcg
     1381 aggccgacaa ctttttcgga gaggtgatcg ggaactggac ggccaccgag ccgctgaagg
     1441 tcgggaacct cgacccggcg tatagtggca acgccacgct cgacctcgtc attcagggcg
     1501 gttcccttat ggagcaccgc atcgccgtcg cgatcaatgg gcacgacctg agcaccgttg
     1561 tcctgcagag gaccgagcag ccgaccttca ccttccagct tccgcagtca tggctggttt
     1621 cgggctcgaa cgatgtcacg ctcacctcgc tgaacggcga cgacgacatc agcgggatgg
     1681 cgaaatcccg tctcacatat caacaccttc tccgcgccga caacggtgcg ctcgatgtga
     1741 gtctcccggg cggcagatcc gtgacggtcg gcggcttcag tgtgaaaact gtccgcgcct
     1801 ttgacattac ggacccgcgg catccaacgg agctccaggc cactgtcgcc gccgatccgc
     1861 agggcggttt cgcagcgacg ttctcgacgc ccgccgacgg catgcgcaca gtgctggcga
     1921 tcgacagctc gcgcttgctc acgcctggag aactgaccgc gaatacacca tcgtcgtgga
     1981 gcgccaccac caccagcgcg aagaattcgg cgtccttctt catcatcagc caccgttctt
     2041 tcctcggcgc cgccgcgaca ttgaaggcgt tgcgggataa ccagggaatc agcaccgcgg
     2101 tcgtcgacgt cgacgatctt tacgacgaat tcaatttcgg gattcgctct ccggaggcga
     2161 tccgttcctt cttccgtgcc gccgccggtt ggaaacatcc accgagtgcg gcacttctcc
     2221 tcggcgatgc cagcatggat ccgcgcaact atctcggcgt ggcttcggtg gacttcgtgc
     2281 cgacgaagct cgttccgacg ttctatatga agacggcgtc ggatgattgg ttcacagact
     2341 tcaatggcga cggcattgcg gacatcccgg tgggccgaat tccggttcgc actcctcagg
     2401 aagcgtcgct ggtcatcggc aagatcacgt cgcgcggtac gccggctggc ccatggtcga
     2461 aaacggcgct gttcgtcgcg gacggcccag ggggcttcga cttccccggt gtcg
//
LOCUS       DATLVW010000015         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_455732, whole genome shotgun sequence.
ACCESSION   DATLVW010000015 DATLVW010000000
VERSION     DATLVW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455732"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..473
                     /locus_tag="VNN25_00410"
     CDS             93..473
                     /locus_tag="VNN25_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH90010.1"
                     /translation="MKILVIEDQPIELKLVGRVLEAAGFTVNGIDAAEQAFASIKADR
                     PQLILLDMGLPGMDGLALVRLLKADPETCAIHVVAVTSYPEEFSRVKALEEGCDGYLL
                     KPISTRTLPQKLSAILGRETRDEP"
     gene            470..3250
                     /locus_tag="VNN25_00415"
     CDS             470..3250
                     /locus_tag="VNN25_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH90011.1"
                     /translation="MKSLKILIVDDVAVNLKLLRVRLEGEGHSIIQARNGVEALKVLD
                     AEGADVIISDILMPVMDGYRLCHEVRRSERFKDIPFIIHTATYTSPADKELSLGLGAN
                     SYLHKPASAQDLMSAISNALNTRHQKPSLSLDGMEVLNQYSERLVSKLEEKNIELTAA
                     VNQLGLQTTALETAADAILITDAQGTILWVNPAFTTMTGYSSDEAIGNTPRMLRSGMH
                     DPEVYRQFWQTINSGQVWRGEFTNRCKDGSIRYDDQTVSPVLGPDGEITHFVGVLHDV
                     TERKRAEEELRNTHAQLRQLLDHSPAVMYALKVDGETITPHLVSENISRLLGFAAEET
                     LSFDWWLGRLHPEDRDRALASLDETLREEVSCTEYRLQHKDGTYRWVEDNRRLIRDER
                     GEPAELVGVWTDVTERRRAQDDLRESERRFSDMLRNLQLVSATLDRDARITYCNDYLL
                     HLTGWNREDVLGKSWFDVFLPADLRAELQETFSSLVADLPESWHQENEIITRAGERRL
                     ISWNNTVLRSAAGEVIGTASIGEDITNRKSLERQLLRTQRMESLGTLAGGIAHDLNNL
                     FMPILMGASLIRRVEPGEATRKAVDNIERCVRRGTDLVKQVLLFARGLEGSRIVIDTA
                     SIVKEVESIAHSTFPKNVTFVTTVAPHLPMLSGDPTQLTQVLLNLCVNARDAMPGGGQ
                     ITVSMGSADVEPQFAAAHGGSGGGRYVVIEVADTGTGIPPEIIDRIFEPFFTTKETGR
                     GTGLGLSTTIGIVRSHGGFLTVSSEVGRGSSFKVYFPALFDGAFAAAEGPENEESLPR
                     GEGELILVVDDERAILEITVQTLTAFGYEVLIAEDGAQAIDTFVLHRSRIALVLTDMA
                     MPVVDGAALIGALRRIDPHVRIIAASGGPADAAKLDKSVTPHFLAKPFTAETLLRTLS
                     RVLREQTLTTRT"
     gene            3298..4293
                     /locus_tag="VNN25_00420"
     CDS             3298..4293
                     /locus_tag="VNN25_00420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH90012.1"
                     /translation="MPNHAETSLAEPTDDTSIELIALRREVEAFLELDSGDGRSEARY
                     HHVLAAVHSICAAIEDCERAGIMRGEIVEILDPVRRIHARSPFVKRLQEWPRGYPGDF
                     ETVEYLCRGPRDVPAQTIAGICEQYSLTRSVAQQHRNKVQHQAARIMRTMLERPGRAR
                     IFSIACGSCPDFRSIALHLPSLLGELWLNDGDADALDFSLRALESVRSRCQVHRGNAL
                     KVVRKAAQEHGSYFDLVMAGGLFDYLPEKHAVYLIEHAYSLLRPGGVLYFTNIARGNP
                     YRPLIEYFGDWFLIERTEDDIRAYCHTLGIPRQAVSITRDETGLALLIEITKQAV"
     gene            complement(4340..>4637)
                     /locus_tag="VNN25_00425"
     CDS             complement(4340..>4637)
                     /locus_tag="VNN25_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HXH90013.1"
                     /translation="PMKQAFIGKILMPFFKSSMLGEKPFSRNGPTDPSFIVSDERDFD
                     VERKKLLELIDRLVERGPAKAAEQTHAFFGRLSGQEWGELMYKHIDHHLQQFGV"
BASE COUNT          973 a         1391 c         1376 g          897 t
ORIGIN      
        1 tgcgacgggt acatcaccaa gccgatcgac acccggaagt tgccggggta tgtagccgaa
       61 tgcctcggcc gccggcaaac aggaaggccg gcatgaaaat cctcgtcatc gaagatcagc
      121 ccatcgagct gaaactggta ggacgcgtat tggaagcggc cggtttcacg gtgaacggta
      181 tcgatgccgc ggagcaggct tttgcttcga tcaaggctga tcgtccgcag ctcatcctgc
      241 tggatatggg tctgccgggt atggacggtc ttgcactggt gcgattgctg aaggccgatc
      301 ccgaaacgtg cgcgatccac gtcgtagccg tcacgtcgta tccggaggaa ttttctcgcg
      361 tgaaagcgct cgaagaggga tgcgatggct atctgttgaa accaatcagt acgcgcacat
      421 tgcctcagaa gctgagcgca atcctgggcc gggagacaag agacgaacca tgaagtcgtt
      481 gaagatcctc atcgtcgacg acgtggcagt caacctcaag ctcttgcggg taagactgga
      541 aggtgagggt cattccatca tccaggcgcg taatggcgtc gaagcgctga aggttctgga
      601 tgccgaggga gcggacgtca tcatctccga catcctcatg ccggtaatgg atggataccg
      661 cctctgccac gaagtacgca ggagcgaacg attcaaagac attccattca ttattcatac
      721 agccacgtac acttcgccag ccgataagga gttatcgctg ggcctcggcg cgaactctta
      781 tcttcacaag cctgcatcgg ctcaggattt gatgagcgcg atcagtaacg cgttgaacac
      841 cagacatcaa aaaccttctc tttcgctgga cggtatggag gtcctcaatc aatacagcga
      901 gaggctcgtg tccaagctcg aggaaaagaa catcgagctg accgctgccg tcaatcagct
      961 cggattgcaa acgacagcgc tcgaaacagc agccgatgca atcctgatca ccgacgctca
     1021 gggaacgatt ctctgggtca atcccgcatt tacgacgatg accggataca gcagcgacga
     1081 agccatcggc aatacacccc gaatgctcag gtccggaatg cacgacccgg aggtctatcg
     1141 ccagttctgg cagactatca acagcggcca ggtgtggcgc ggcgaattca cgaaccgctg
     1201 caaggatggc agcattcgct acgacgacca gacggtcagt cctgtgctcg gcccagacgg
     1261 tgagatcacg cacttcgtcg gcgtgctgca cgacgtgacg gagcgaaaac gggctgagga
     1321 ggagctccgt aatacccacg cacaattacg tcaattgctc gaccacagtc cggccgtcat
     1381 gtacgcgttg aaggtcgatg gcgagacgat cacgccccat ttagtcagtg aaaacatcag
     1441 tcgattgctc ggatttgccg cagaggagac gctgagcttc gattggtggc tgggacgatt
     1501 gcaccccgag gatcgggatc gggccttggc gagtctggac gaaacactcc gcgaggaagt
     1561 gagctgcacg gaataccgct tgcagcacaa agacggtacg tatcgctggg tggaagacaa
     1621 tcgccgcttg atccgcgacg agcgaggcga gcccgcggag ctcgtcggcg tctggaccga
     1681 cgtcacggag cgccggcgcg cgcaggacga cttgcgggaa agcgaacgcc ggttcagcga
     1741 catgctccgg aacctgcagt tggtttcggc cacgctcgac cgcgacgcac gcatcaccta
     1801 ctgcaacgac tatctccttc acctcaccgg ttggaatcgc gaagacgtgc tcggcaagag
     1861 ctggttcgat gtcttcctgc cggccgacct gcgggccgaa ttgcaggaaa cgttctcctc
     1921 tctggttgct gacctgccgg aatcgtggca tcaggagaac gagatcatca cccgagccgg
     1981 ggagcggcgg ctcatctcct ggaacaatac ggttcttcgc tcggccgcgg gcgaagtgat
     2041 tggcacggcc agcatcggcg aggacatcac caatcgtaag agtctcgaga ggcaacttct
     2101 gcgaacacag cgcatggaga gcctgggcac gctcgccggc ggcatcgccc acgatctgaa
     2161 caatctcttc atgccgatcc tcatgggagc gtctctgatc aggcgggtcg agcctggcga
     2221 ggccacgcgg aaggcggtcg acaacatcga gcgctgcgtc aggcggggaa ccgatctcgt
     2281 gaagcaggtt ctcttgttcg cgcgaggact cgagggctcg cgaatcgtga tcgacaccgc
     2341 cagcatcgtg aaggaggtag agtcgattgc ccacagcacc ttcccaaaga atgtgacatt
     2401 cgtaaccacc gttgctcccc atcttccgat gctttcaggc gatccgacgc aattgaccca
     2461 ggtgctcctg aacctttgcg tcaacgcccg tgacgcgatg cctggcgggg gacagatcac
     2521 agtctcgatg ggaagcgccg acgttgagcc ccaatttgcc gcggcgcacg gcggatcagg
     2581 cggcggaagg tatgtcgtca tcgaggttgc cgataccggc acagggatac cgccggagat
     2641 catcgatcgg attttcgagc cctttttcac caccaaagaa accggccgcg gcaccggcct
     2701 cgggctttcg acgacgatcg gaatcgtgcg cagtcacgga ggatttctca ccgtttcgag
     2761 cgaagtcggc cgaggcagtt cgttcaaggt ctacttcccc gcgcttttcg acggcgcctt
     2821 tgcagccgcc gagggtcccg agaacgagga gagtcttccg cggggagagg gcgagctgat
     2881 tctcgttgtc gacgatgagc gagccattct cgagatcacc gtccagacgc tcaccgcatt
     2941 cggctatgaa gttctcatcg ccgaggatgg tgcgcaggcc atcgacacat ttgtcctcca
     3001 tcgatcgaga atcgcgctcg ttctcacgga catggccatg ccggtcgtcg atggcgcagc
     3061 gctcatcgga gctctgcgcc ggatcgatcc gcacgttcgc atcattgccg caagcggtgg
     3121 accggccgac gctgccaaat tggacaagag cgtgactccc cacttcctgg ccaagccttt
     3181 tacagcggag acccttctgc gcacgctgtc cagagtgctt cgagagcaga cattgacgac
     3241 cagaacttga cgctcgacgc gggcgaaaaa tggaatgttg tccgtctctg ctaaggcatg
     3301 ccgaatcatg ccgagacgtc cctggcagaa ccgacagatg acacctctat tgagctgatc
     3361 gctcttcgcc gtgaagtcga agcgtttctc gagttggact ccggcgatgg gcgctccgag
     3421 gctcgctacc atcacgtgct cgccgcggtg cacagcatct gtgccgcgat cgaggactgc
     3481 gagcgcgccg gaatcatgcg cggcgagatc gtcgagatcc tcgatccggt gcgccgcatt
     3541 catgcccgct cgccattcgt aaaacgcctg caggaatggc cgcgcggtta tcccggagac
     3601 ttcgagacgg tcgagtatct ctgccgcggt ccgagggatg ttccggcgca aacgatcgcc
     3661 gggatctgcg agcaatactc gctgactcgt tcggtcgcgc agcagcatcg caacaaagtg
     3721 cagcaccagg ctgcgcgcat catgcgcacg atgctcgaac gccccggcag agcgcgcatc
     3781 ttctcgatcg cctgcggcag ttgtcccgat tttcgcagca tcgcgctgca tcttccgtcg
     3841 ctcctcggcg agctttggct gaacgatggc gatgccgacg cactcgactt ctcactccga
     3901 gcgcttgaat ctgtcaggag ccgctgtcag gttcatcggg gcaacgcgct gaaagtcgtt
     3961 cggaaagccg cgcaggagca cggctcgtac ttcgatctcg tgatggccgg cggtctgttc
     4021 gactacctgc ctgagaagca tgcggtctac ctcatcgaac atgcgtattc gctcttgcgg
     4081 cccggcggtg tcctctattt caccaacatc gcccgcggca atccgtatcg cccgctgatt
     4141 gagtacttcg gcgactggtt cctgatcgag cgcaccgaag atgacatccg cgcctattgc
     4201 cacaccctcg ggatcccgcg ccaagccgtc tcaatcacgc gcgacgaaac cggtctggcg
     4261 ctcctgatcg agataacgaa acaggccgtt tgacagaccg tgtagttcat tcttgtcggg
     4321 ccgttcgaac cgcggcgggc tacaccccga actgctgcaa gtgatgatcg atgtgcttgt
     4381 acatcaactc gccccactcc tgaccgctca gtctcccgaa gaaggcgtgc gtctgttcgg
     4441 cggctttcgc agggcctcgc tctaccagac gatcgatgag ctcgagcagt ttcttgcgct
     4501 caacgtcgaa gtcgcgttcg tcggagacga tgaatgacgg atcggtggga ccgttgcggc
     4561 tgaacggctt ctcaccaagc atcgaggact tgaagaacgg catcaagatc ttgccgatga
     4621 acgcctgttt catcggc
//
LOCUS       DATLVW010000016         8902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_331914, whole genome shotgun sequence.
ACCESSION   DATLVW010000016 DATLVW010000000
VERSION     DATLVW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331914"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VNN25_00430"
     CDS             complement(<1..306)
                     /locus_tag="VNN25_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXH90014.1"
                     /translation="MSGFNLIEPATGEPFATIPRATADDADAAIRRAAAAFPKWRAVN
                     SRSRGRILMRLSQLIRDNADELAKLESRNVGKPIRDSRDEVALAANCFEYYAGTTNKI
                     "
     gene            complement(1088..2314)
                     /locus_tag="VNN25_00435"
     CDS             complement(1088..2314)
                     /locus_tag="VNN25_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXH90015.1"
                     /translation="MATRFEFLREIGQGGLGSVYRGNDRETNQQVAIKMLPHSAGDPP
                     YLVHEYDVLSHLSHPNIVHVIDSFHFDGRLCFAMEMIDGVTLDRALEHEPKSCLSNNA
                     RRVFGQLHDALEYLHESGIVHGELFHGNILISSEGVLKIVDFELARRRDGRDSELFPP
                     GVFMGIPIYSSPEHFTGGTLLPESDYFVVGILLAEHLVRAHPFSVAKFPEILRQIATA
                     EFRPDFAIDSIEPVIREVIARLVSVDLARRREGWELLREQTRLDAVERSARRPDATSN
                     LLPDKGVFISHASADKPVARRIARELSDAGLAVWLDEWRIKPGESVSRSIDDALESCS
                     HVLLLLSTSAVRSPWVEREWRAAYWKEIESARVFLIPVLVSQCAIPPLLRDKKYVSLN
                     IWEEAIAELLEAITGA"
     gene            complement(2639..3568)
                     /locus_tag="VNN25_00440"
     CDS             complement(2639..3568)
                     /locus_tag="VNN25_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXH90016.1"
                     /translation="MDATRRISELPLDSERKSSIIDIPDQVPPDEIASRLIDRDFAVA
                     IVVLDTGDTKLSAEVVRRTAVINAIPAAIPPARFLVTSRSDRSLAPSDELLRDLARRY
                     NFERWVPTSSREGSGIQELRTAILESVAARVLEDKPENEVTRVVRTMTETLCKLIAKN
                     PQVIDEIEWRDLERVVALALQRIGFEVELTPPAKDGGKDVVATFAVRDERKVFYVEVK
                     HWRSGDRPGLRHVTDFIEVNATDGTDGGLFLSSSGFVTAVHGRLAEISAQRVRLGQRG
                     KIVHLCQQFVKSTAGVWRSASILPALLFEETLE"
     gene            complement(3904..5529)
                     /locus_tag="VNN25_00445"
     CDS             complement(3904..5529)
                     /locus_tag="VNN25_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH90017.1"
                     /translation="MRFPLIAVAALALQLSESIEVRVVNVDVVVADRDGKPVTGLTKD
                     DFEIFEDKKPQVITNFYEVRGGVPAGTGAETGSTALRPRSFIFFIDNRAMPPVLRKHV
                     TAEMAKFVDTVVGPNDQVSVVIWDGTFRILTPLTNEMAAVRKAIDTVARTGTPMSATS
                     DFQRVQAECTHALNMGTGREYPGIPIKLAYDQCIGSVRIETQRMTMYSRLMLNAVEVA
                     MSTVAGVEGRKVLVLAGTELPARPGLDMYQWANGRFQPYMRGFDAAIEQPPPEYRDQQ
                     RATLESIAKTANANGVTLYPISALMAADINSTQNSTGIDDAGAGFLRSGNTDAAHELL
                     ARMTGGTAAPVSQMPALLDTLRRDLDSYYSLGYRPSIDQRGDRPIAVRARNRAYTVRA
                     RQSYASKTFDEQMRDRVIANIYTPARVSDWKLQLRTGKPKPVKKGEYSLPIEIVAPPS
                     VTLLPRDGKLAGGLVVYLVVGNAQGALSTTFRQVNGIEITPTEEAVFRRTPLTFGANL
                     TIREGENLISVGLVDQAAGTMGFARTTVTASAK"
     gene            complement(5813..6772)
                     /locus_tag="VNN25_00450"
     CDS             complement(5813..6772)
                     /locus_tag="VNN25_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH90018.1"
                     /translation="MARPLRLEFADALYHVTSRGNERRPIFRTIRDRQQFLLFLGIAV
                     KRFGWSLTAWVLMTNHYHLVMQTPDPNLSKGMHWLNSRYANWFNRTHGRCGHLFQGRF
                     KALLVDREAYFAEVLRYVVLNPVRANLCARPEEYRWSSYRSTAGLEIAPDWLDVEPVY
                     RLFGADKKTAQPLYRDFVLAKIGCEDRLWDKLTNQLYLGTEEWCKTMRAQVESKVRST
                     DHPQVQRGVGRPKMHQVMTAVAKAAGETVETIRHTQGHILRSLVAWLGWHEGWVTLRS
                     IAAVLRLRSEGHISYLIKRCETVFAENTILLEQHDRALAALRA"
     gene            complement(7016..8491)
                     /locus_tag="VNN25_00455"
     CDS             complement(7016..8491)
                     /locus_tag="VNN25_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90019.1"
                     /translation="MDKKMVSDAYCHRPSIFEVNAMRNRGVALALLLLPAFAYAQVDW
                     SPGGGVSGKIALGNPSKAAVLLFHGLGETGTSTFITPSLMSVNWDYVNDPGDRNLGGG
                     HLKAGVGVLDFGVSNSLPVDQKAFFGAMMQSGFTVATWDQPGTSFAQALPSALAAFDK
                     FVADTAALNPTSPPPIALVAHSRGGLLARAVLKQRGSLGRVRWLITLHTPNGGSDMAS
                     FASRCGTDWPAGVDLGPFNPERDTIIATIRGALAPIDAKFNNPAQAELAPGSPLLMAL
                     ATGESKRPFVRYISFGGTSPRFLKMYAWTFVEPGSTIATGHNGLNPTFSWVVHATEMP
                     HIVSPMLDALPNCTPELTPGAGDGLVTDARSHLPFEDSHVTNPFNHVEVLWSPLVQGI
                     ISNELLMQLQPFPITVVPASTSTSYGLTLQFSINPANIVAVNWSVQSGPGRITTDGVY
                     HAPLPHRCWPWNPCPRGLTAVVAATARDDSGRFALASVTLN"
     gene            8696..>8902
                     /locus_tag="VNN25_00460"
     CDS             8696..>8902
                     /locus_tag="VNN25_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90020.1"
                     /translation="MKQNVPLTISSLLSIVLFTVHLAHDIVFGLDSMTRAGTFTFFAI
                     MLVSLYGTVELAGRRSGYVIMLLLG"
BASE COUNT         1794 a         2667 c         2697 g         1744 t
ORIGIN      
        1 gatcttgttc gtcgtccccg cgtagtactc gaagcagttg gcggcgagag caacctcatc
       61 ccgcgaatcg cggatcggct tgccgacgtt gcgcgactcc agtttcgcca gctcgtccgc
      121 attgtcgcga atgagctgcg acagccgcat cagtatccgt ccgcggctgc gcgaattgac
      181 cgcgcgccac ttcgggaagg ccgccgcagc gcggcggatc gcagcatcgg catcgtcggc
      241 ggtcgctcgc ggaatcgtgg cgaacggttc cccggtggca ggctcgatca gattgaatcc
      301 ggacacgcga tcagcctagt gcaaaaccca agctcagggg gaaggcagaa agcggaaagc
      361 aatgtccgcc caatgttccg cggttcagcg gcttcatacc gcgcgcaaac tgtgaaacca
      421 agagtaacgg catgaagtcc accgcaaccg cttagttggg cagcgggtgg gatcgcgtgt
      481 ggccgaggcg attctggcta ccgtctcggc cacccaacta gacgaacatg gtcagcaaat
      541 cggcgagttc cgcgcgccag aaactaagga ctgtggctgc gactcgaacc gcgagccgag
      601 cgccagccag gactgcagaa agccaaagca caaaccgaat gatcgctacg aagcgcgaat
      661 ccaacgaagt cttcatgttg agaactcctg ggctgcagtg ggtgtggtcg ttcgttacaa
      721 gcacgaagca gcgcgtcaca gttgccacga gcctctcgcg acaggagttg cgctgatgtg
      781 cgttgcacgc ggccctaccg cggtgcctcg cccagcggtc ggctcgcgcc ctgcgcgatc
      841 aaccgcgcga cctaccgctt gaggtagatc gggggaccgg tccagcccag ctggcattcg
      901 gttcgtcgac agtatgccac aacgagaagg caaacgacct tccgctgccc aaggccaaac
      961 ggttcgcccg caagacgcct tcagttgtgc cgcggcgtac gcggcgtgaa gccgtcggtc
     1021 tcgcggccca agaacaggct cggccgacgg gcgcggaata cggcgtcttg tcgggtgcta
     1081 ccgtgaatca ggcgccggtt atcgcctcga gcaactcggc gatagcttcc tcccagatgt
     1141 tcagcgacac gtatttcttg tctcgcaaaa gaggagggat agcacactgg ctgaccaaca
     1201 ccggaataag aaaaacgcgc gcagattcga tctctttcca gtacgctgcg cgccactcgc
     1261 gctcaaccca tggcgaccgt accgcgctcg tcgacagaag caacaggacg tgactgcatg
     1321 actcgagcgc gtcatcgata ctccttgaaa ccgactcccc aggtttgata cgccattcat
     1381 ccagccatac agcgagccca gcatccgaca gttcgcgagc aattctgcgc gcaaccggct
     1441 tgtcggccga agcgtgagaa atgaagacgc ctttgtcggg aagcaaattc gacgttgcat
     1501 caggccgacg tgctgaacgc tccacagcgt caaggcgtgt ttgttcacgc agcagttccc
     1561 atccttctcg gcgccgggca agatcgaccg agaccagtcg ggcgatcact tcgcgaatca
     1621 ccggttcgat agaatcgatc gcaaagtcgg gtcggaactc tgccgtagca atctgtcgca
     1681 gaatttctgg aaacttcgcc acgctaaagg gatgagcccg caccagatgt tcggccagta
     1741 gtattccgac cacaaagtag tcggactccg gcaacagcgt accgccggtg aagtgttcgg
     1801 gcgatgaata gattggtatg cccatgaaga caccgggcgg aaacaattca gaatcgcgac
     1861 catcgcgtcg tcgagcaagc tcgaagtcta ctatcttcag aaccccctcg gacgaaataa
     1921 ggatgtttcc atggaatagt tcgccatgga cgatgccgct ttcgtgaagg tactcgagcg
     1981 cgtcgtgaag ctgaccaaag acgcgtcgtg cgttattgct tagacacgac tttggctcat
     2041 gctctagggc gcgatctaac gtcacaccat cgatcatctc catcgcaaaa cagagtctgc
     2101 catcaaagtg aaagctatcg attacatgca cgatattcgg gtgagagaga tgcgacagaa
     2161 catcgtactc gtgcactaga tatggaggat cgcctgcgct gtgaggaagc atctttattg
     2221 cgacctgctg gttcgtctcg cggtcgttgc cgcgatagac gctgccgagg ccaccttggc
     2281 cgatctcgcg cagaaactcg aaccgggtcg ccattttctc acgtgcgcct aacgtctcca
     2341 ggtgagcttc ggcccacacg gaggacgtac acgtaagcgt atcagatgct gggctagagt
     2401 ccgctccacc gccgagttgc gcggcatttg cgcgacgcgt ttagccccgc ccgtgtctgc
     2461 gtctcatgaa ggtacttcca gtccggaacg ttcccaagca acgccgactg agttcctttg
     2521 gcgaatgtgt agatcgaaac gtctggtttc cacgcgcgag gagagtacct gagcacctcg
     2581 actaccagag ttgccgcagc ttcaaaccga agttgggcgt gcccgcggaa ggatctcact
     2641 attcgagcgt ttcttcgaag agcagcgccg gcagaatact cgcagatcgc cacacacccg
     2701 ctgtactctt cacaaactgc tgacaaaggt gaacaatttt accccgttgc ccgagccgca
     2761 cacgttgagc tgagatctca gcaagccgac catggacagc ggttacaaat ccagatgacg
     2821 aaagaaacaa cccgccgtca gtaccatcag ttgcgttcac ctcgataaag tccgtcacgt
     2881 ggcgcagtcc gggtcgatcg ccgcttcgcc aatgcttgac ttcaacatag aaaacttttc
     2941 gctcatcgcg gacggcgaag gtcgccacaa catccttgcc accatctttg gccggcggcg
     3001 tcaactcgac ttcgaaaccg atcctctgta gcgctaaagc gaccactcgc tcgagatcgc
     3061 gccattcgat ttcatcaatg acttgtgggt ttttggcgat cagtttgcag agcgtctccg
     3121 tcatagttcg gacgacgcgt gtgacttcat tctcaggctt gtcttccaaa acacgagcag
     3181 cgaccgactc gagtatcgcc gttcgcaatt cttgaatacc tgagccctct ctcgaactgg
     3241 taggaaccca tcgttcaaaa ttgtagcgtc tcgccaaatc acgcaagagt tcgtcgctgg
     3301 gtgcgagaga gcgatcgctg cgagaagtga cgagaaaacg tgcgggcggt atggcggcag
     3361 ggatcgcgtt gatcactgca gttcgtctca ccacttctgc tgacagttta gtatcgcctg
     3421 tgtccaagac aacgatggct acggcaaaat cgcgatcaat taaccggctc gcgatttcgt
     3481 ctggaggaac ttgatccgga atatcgatga tgctggactt ccgctccgaa tcgagtggca
     3541 actcggatat acggcgcgta gcgtccatcg accggaaagc ctcacccatt agagaacgcg
     3601 cgagagacga tttgccgacg ccggaaggtc ccatgaggac gactacggca gacggcctgc
     3661 tggatgatgc tgcggaagcc ataagttcgc gggggatagc atatcgggag gtgccgaggg
     3721 tcacaccttc ggtcgttcgg ctgacgactc tcaaaacgct aggttccaat cggagacttt
     3781 tccgcgacat cgtggccggt ggccgaaact catcggaatc gagatcgcgc tacgatgcgt
     3841 agagagtctg agccagaacc cgcggagcga aggtgtgacc ctattacgtc gtcctgctct
     3901 tgactatttg gcggaggcgg tcacggtcgt tcttgcgaag cccatcgttc cagccgcctg
     3961 gtcgaccagg ccgacagaga tgagattctc accttcgcgg atggtcaggt ttgcgccgaa
     4021 ggtcaggggc gtgcggcgaa agacggcctc ctcggtgggg gtgatctcga ttccattcac
     4081 ctggcggaag gtcgtcgaca gcgcgccctg ggcgttgccg acgacgaggt agaccaccaa
     4141 tccgccggcg agcttgccgt cgcgcgggag cagagtgacg ctcggcggcg cgacgatctc
     4201 aatggggaga ctgtactccc ccttcttcac cggcttcggt ttcccggtgc ggagctggag
     4261 cttccagtcg ctcacgcggg ctggggtgta gatatttgcg atcacgcggt cgcgcatctg
     4321 ttcgtcgaag gtcttggagg cgtagctctg gcgcgcgcgg acggtgtagg cgcgattcct
     4381 ggcgcggact gcaatcgggc ggtcgccgcg ttggtcaatg ctggggcggt agccgaggga
     4441 gtaatacgag tcgagatcgc ggcgcagggt atcgagcaga gccggcatct gcgagaccgg
     4501 cgcggccgtg ccgccagtca tgcgcgccag cagctcgtgc gcggcgtccg tgttgccgga
     4561 gcggagaaac cccgcgccgg cgtcgtcgat cccagtggag ttctgcgtcg agttgatgtc
     4621 ggccgccatg agcgcggaga tcggatagag cgtcacgccg ttcgcgttcg cggttttggc
     4681 gatgctctcc agcgtcgcgc gctgctggtc gcggtactca ggcggcggct gctcgatggc
     4741 ggcatcgaac ccgcgcatgt acggctggaa ccgcccgttc gcccattggt acatgtccag
     4801 ccccggccgc gcgggaagtt ccgtgccagc caggacgagc accttccgac cctctacgcc
     4861 cgccaccgtc gacattgcca cctcgacggc gttgagcatc aggcgcgagt acatcgtcat
     4921 tcgctgcgtt tcgatgcgca cgctgccaat gcactggtcg taggccagct tgattggtat
     4981 gccggggtat tcgcgaccag tgcccatgtt caacgcgtga gtgcactccg cctgcacgcg
     5041 ctggaagtcg ctcgttgccg acatcggcgt gccggttctg gccacggtgt cgatggcctt
     5101 tcgcaccgcc gccatttcgt tggtcagcgg cgtgaggatt ctgaatgtgc cgtcccagat
     5161 gacaaccgag acctgatcgt tcggaccgac gaccgtgtcg acgaacttcg ccatctcggc
     5221 ggtgacgtgc ttgcgcagca ccgggggcat ggcgcggttg tcgatgaaga agatgaagct
     5281 gcgcggccgc agggcggtcg agccagtctc ggcgccggtt ccggccggaa cgccgcctcg
     5341 gacctcgtag aagttcgtga tgacctgcgg ctttttgtcc tcgaagatct cgaagtcgtc
     5401 tttggtcagg ccggtgaccg gcttgccgtc gcgatcggca accacgacgt cgacgttgac
     5461 gacgcgcact tcgatcgact cgctgagttg aagggccagc gctgccacgg caatcagagg
     5521 aaagcgcacg gcaaaggcta ctcgaaccag gacactcgct tcatctcttt tgccagaggg
     5581 gtcacatctc ttttgccaga ggggtcacac cttcggtcgt tcggctgacg atgatcaaac
     5641 ggtatgtttc aatcggaccc ttgcggcgat ggcgacatcg cggccagtgg ccggccaatt
     5701 atgaggtgat attggacgga gttcacctgc cgcaaggagc gttctacgac cctcgtgacc
     5761 gcgttcgggc acacgtccgc ctgctacggc gttgaaggcg caaatcgccc gctcaggcgc
     5821 gaagcgcggc cagtgctcgg tcgtgttgct cgagaagaat ggtgttctca gcgaacaccg
     5881 tctcgcatcg cttgatcaga tacgaaatgt gtccttcgct tcgtaatcgg aggaccgctg
     5941 cgatcgagcg gagcgtgacc cagccctcgt gccatcccag ccaagccacc aggcttcgca
     6001 gaatatggcc ttgcgtgtga cgaatggttt cgacagtctc accggccgct ttcgctaccg
     6061 ccgtcatgac ctgatgcatc tttggccggc ccacgccccg ttgtacttgg ggatgatcgg
     6121 tcgaacgtac cttggactcg acttgtgcgc gcatcgtctt gcaccattcc tcagtgccga
     6181 ggtagagctg attggtcagc ttgtcccaga ggcgatcctc gcatccgatc ttcgccagaa
     6241 cgaaatcgcg gtagaggggc tgcgcggttt tcttgtccgc accaaacagg cgatagaccg
     6301 gttcgacatc cagccagtcg ggtgcgatct cgagtcccgc ggtggagcga tagctcgacc
     6361 agcgatactc ctcggggcgc gcgcacagat tcgcgcgcac gggattgagg acgacatacc
     6421 ggagaacctc cgcgaagtac gcttcccgat cgacgagaag cgctttgaat cgtccctgaa
     6481 acagatggcc acagcggccg tgggttctgt tgaaccagtt tgcgtatctg ctgttgagcc
     6541 agtgcattcc tttcgaaaga tttggatccg gggtctgcat gacgaggtga tagtggttcg
     6601 tcatcagcac ccacgcggtc agggaccagc cgaaccgctt caccgctatg ccgaggaaga
     6661 ggaggaactg ctggcggtcg cggatggtgc gaaagatcgg ccgccgctca ttgccgcggg
     6721 aggtaacgtg gtacagcgca tcagcgaatt cgagtcgtag tggtcttgcc ataatgtcaa
     6781 cgaggatccc tcggcaaacg catagctccg aggaaatcca aattgcgtag aaaaccgggc
     6841 tggcaaactc ctcggaatgg aagatcgcgc tacgacccgt agagagagtc tgaggcagga
     6901 cccgcggagc gaaggtgtga cccccactgt ttcgcggagc gaaggtgtgg ccccattgtc
     6961 ctgtccttgt cgtccttcct gttccgcgga gcgaaggtgt ggcccattgt cgccttcaat
     7021 taagtgtcac actcgccaga gcgaaacgac ccgagtcgtc ccgcgcagtt gcggccacca
     7081 cggcggtcag accgcgcgga catggattcc acggccagca tcggtgtgga agcggcgcgt
     7141 ggtacacacc gtccgtggtt atccgtcccg gcccgctttg gaccgaccag ttgacggcga
     7201 cgatattcgc aggattgatg ctgaattgaa gggtgagccc atagctggtg ctcgtgcttg
     7261 caggcacgac cgtgattgga aacggctgca gttgcatcaa caactcgttc gagatgatgc
     7321 cctgtacgag cgggctccaa agcacctcga cgtgattgaa cggattcgtg acgtggctat
     7381 cttcaaaggg aaggtgggag cgggcgtcgg tcaccaggcc gtcgcctgct cccggcgtca
     7441 gctccggcgt gcagttcggc agcgcgtcga gcattggcga aacaatgtgc ggcatttcgg
     7501 ttgcgtggac tacccacgaa aatgtcgggt tcagtccatt gtgcccggtg gcaatcgtgc
     7561 tcccgggctc cacgaaggtc caagcgtaca ttttcaggaa gcgtgggctc gtcccaccga
     7621 acgaaatgta ccgcacgaat ggacgcttgg attcgcccgt ggcgagggcc atcagcaacg
     7681 gacttcccgg ggcgagctca gcctgcgcgg ggttgttgaa tttcgcatcg atgggagcga
     7741 gcgctccgcg gatcgtggcg atgatcgtat cgcgctccgg gttgaacggg ccgaggtcga
     7801 cgccggcggg ccagtcggta ccgcatcggc tggcgaacga ggccatgtca ctgccaccat
     7861 tcggcgtgtg cagcgtaatc agccaccgaa cgcgtcccag cgatccgcgt tgcttcaaca
     7921 ccgcgcgcgc cagcagaccg cctcggctgt gagccacgag cgcgatcggc ggtggcgacg
     7981 tcggattgag tgccgcggtg tccgctacga acttgtcaaa cgccgcgagc gctgacggga
     8041 gcgcctgggc gaacgacgtc cccggctggt cccatgtggc cacggtgaat ccactctgca
     8101 tcatcgcgcc gaagaacgcc ttctgatcaa cgggaagaga gttggacact ccgaagtcca
     8161 ataccccgac ccctgctttc agatgaccgc cacccaggtt gcggtccccg ggatcattca
     8221 cgtagtccca gttcacactc atgagcgaag gcgtgatgaa cgtgctcgtg ccggtttccc
     8281 ccaggccgtg aaacagaagg acagccgcct tcgacggatt gccgagtgcg atttttccgc
     8341 tcacgcctcc accgggagac caatcgacct gagcgtacgc aaacgccggg agcagcaaca
     8401 gagcaagggc aacaccgcga tttcgcatgg cattcacctc gaaaatggaa ggacgatggc
     8461 aatatgcgtc gctcaccatc tttttgtcca ttaccggctc cacgcgaatc cgttcgtgac
     8521 gcacggattg cgtccgcgga gcgaaggtgt gaccctattg tggcggcttc gacgacgacg
     8581 ggttcgcgcg caaaccgctt gctgaaaacg aaacccacgc gcaaaacagt cgtagcaatc
     8641 ccacaggacc ggtcgtcttc ccgaacaggg cgcgcacgcg ccggcaggag ctccgatgaa
     8701 acagaacgtg ccactgacca tctcttcgtt actgtcgatc gtgctattca ccgtgcatct
     8761 tgcgcatgac attgtttttg gcctggactc gatgacgcgc gccggcacgt tcacgttctt
     8821 cgccatcatg ctcgtgtcac tttacggaac ggtggagctg gccggacgcc gatcgggcta
     8881 cgtcatcatg ctgctgctcg gg
//
LOCUS       DATLVW010000017        24536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_166292, whole genome shotgun sequence.
ACCESSION   DATLVW010000017 DATLVW010000000
VERSION     DATLVW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24536
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166292"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VNN25_00465"
     CDS             <1..850
                     /locus_tag="VNN25_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXH90021.1"
                     /translation="VLAAYRAASKHAYLPKLMEHNDAAMLQQDVGDQTLFDLLHRDRE
                     EGVGWYRKAVTLLAYFQKAGAVDINPPFTGDFFFNELEMSREFYVEKLMRVSPESAQP
                     LKPLLRKLADNISRHPYVLCHRDYHGQNIHIFNDKLYLIDYQDLRMGPDTYDIASLLR
                     DRGVARILGEETELELVDYYAEWRVHGLPARSELPQGERRSMRRRYFETLLQRSIKIL
                     GTFSKQPLTRGRMWYLDFIPPTLESVRRCIGELPEYGPLAALLPMDFSLSEARERAER
                     IHHGST"
     gene            837..1865
                     /locus_tag="VNN25_00470"
     CDS             837..1865
                     /locus_tag="VNN25_00470"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HXH90022.1"
                     /translation="MVRHKITLLPGDGIGPEVTASVVSIIECAGVDVEWEKFFVGAEA
                     LSRFGDPLPGDVLESILRNKVALKGPVTTPIGTGFSSINVRLRKTLDLYANLRPVKSM
                     PNIITRYENVDIIVVRENTEDLYSGLEHEVVPGVVESIKIITERASTRIARFAFEYAR
                     THGRKRVTAVHKANIMKLSDGLFLQCFRNVAKEYPNIEADDKIVDNLCMQMVMNPIQF
                     DVLLLENLYGDIVSDLAAGLVGGLGVVAGANIGENGAVFEAVHGSAPDIAGQNKANPL
                     ALLQSAVQMLHHIGESGAAKRVDDAITKVLDSGPEHRTRDIGGIGTTADFTTAMCETL
                     RRGTRAGD"
     gene            1923..2327
                     /locus_tag="VNN25_00475"
     CDS             1923..2327
                     /locus_tag="VNN25_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXH90023.1"
                     /translation="MIDFETHFRAGLDHFNAGEFWNAHEAWETIWLEAESEVHVFLQG
                     LIQLAAAYHHVKRGTYPGGLRLFDAALQKLEGFPKRWCGIDRTSAELAAHRHREWVAD
                     VLARNAKAERLDANDFPRLVPDDSPIPPFEHW"
     gene            2398..3408
                     /locus_tag="VNN25_00480"
     CDS             2398..3408
                     /locus_tag="VNN25_00480"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXH90024.1"
                     /translation="MQEIDQRDRELLGALQSEIPLVSTPFALIGQAIDMSEKEVIKRI
                     ERLKREGLLRQLAAQFDARALGYRSCLVAAKVDPERIDDAAAMINAHPGVTQNYRRNN
                     DFNLWFTIAVLSSSSLGLNRTIEILGSEAGCETVRALPTLRQFKGNSDTDESQSDTQF
                     APLTSVEIESVRLLQRDLPLQPRPFEAMAKTNGIGADELLAAAKSLLQRGYIRRYSAV
                     VQTRKTGFGASAMGVWIVPSDAVEDTGTKMSQNKAVSHCYLRPVYDDWPYNLYTIVHG
                     RSVDECESIINDLAIDTGVESRQALYPTREYKKARINLFSPEVEEWETAHGGSQSASA
                     AS"
     gene            3451..3741
                     /locus_tag="VNN25_00485"
     CDS             3451..3741
                     /locus_tag="VNN25_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016914159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH90025.1"
                     /translation="MTDIPELTSAQFSKAIPARVRKRLMRGEFKSGGDVTALRRFLGL
                     TQVELARAMSISVYTLRGWEQGRRSPEGPAIALLRIAARHPRVIRENLRSAA"
     gene            complement(3812..4783)
                     /locus_tag="VNN25_00490"
     CDS             complement(3812..4783)
                     /locus_tag="VNN25_00490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXH90026.1"
                     /translation="MIKRMLFISLLALPAAAQQSAPAQPPAAQSAAVAVPDSKKPVAV
                     INGETVTAEKLDRMYSNLNTQMRQNYDANGGKGALLENYIRKRLLIQEAIKSGYNKRP
                     DVIEAVDAAREAALFDRYVRDVVAAQIVTDNDVKTYYTEHPDDFATPEKVHVRHIVIG
                     VTEVGPARKTKEEALDRIKKIAVDLRSEDVASAQSTSNPEVLARLRLAHFADAAKKYS
                     EDASGESGGDLGWITKGQTDPDFETAAWSMKPGTVSGIIQTKFGFHLIFVEGRQAPGT
                     EPFESVKSSIREFLITQKASDVVTTVTKLTNELRGNSRISVFPENIK"
     gene            complement(4855..5493)
                     /locus_tag="VNN25_00495"
     CDS             complement(4855..5493)
                     /locus_tag="VNN25_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90027.1"
                     /translation="MRRSRHPEYERNVFINCPFDDEYTPIFEAVVFTIQACGFRTVCA
                     RSRLNSSEVRLEKILEIIDDCRYSIHDLSRTELDRASALPRFNMPLELGIDIGCRAYG
                     DDQEDKSFLIFDRDRFRFQTYISDIAGQDIANHEDDPNLAIARVRDWLRAETGRTNIP
                     GAKAMQSKYASFRKELPSICERFDLDILSLTFADFMHLVGDWLTPYLAPPFR"
     gene            complement(5504..5818)
                     /locus_tag="VNN25_00500"
     CDS             complement(5504..5818)
                     /locus_tag="VNN25_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL30 family ribosomal protein"
                     /protein_id="HXH90028.1"
                     /translation="MADATKPKKFRLRRLRGKTIRLKQIRSATGSPRTVRETLKALGL
                     GKLYQFAELPNTKEVRDLITKAPHMLSILDGPDILFPLEKSTLGAERIREAVEAVRAL
                     RK"
     gene            6064..7461
                     /gene="dnaB"
                     /locus_tag="VNN25_00505"
     CDS             6064..7461
                     /gene="dnaB"
                     /locus_tag="VNN25_00505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXH90029.1"
                     /translation="MSVAQLDVALDRPMPQSPDAERAVLGSILINNHAFYRVVGTINT
                     EDFFRDAHRTIFATMRRLAEQSREIDPLTLREELSKNAQLEQVGGSAYVASLVDGIPD
                     IANVERYARIVKEKSTLRRLIVMGNSVMRAALDAPSEPADVLNIAEKSIYEIAEGSIE
                     KGFVALDRITRTNMTAIEQLQHAGKLLTGIPTGYDRFNEFTSGFQNQDLIIIAARPSM
                     GKTSFMMNIAESIAIPERSGGPRPPAQRLYSVGVFSLEMSKEQIGLRILSSESGVSNH
                     LIRAGMLSERNWRDLADASARLAKAKIFIDDTPGMDIMEMRAKARRLKMEAGLDMIMV
                     DYLQLMAVKGKTESRNQEISQISRGLKAIAKELNVPLISLSQLSRRPEQRTGDHRPQL
                     SDLRESGSIEQDADVVCFIYRDEVYNKDTEEKGIAEIIIGKQRNGPIGDFKLVFRNDI
                     TKFFNYEPTPDYGPQ"
     gene            7509..8777
                     /locus_tag="VNN25_00510"
     CDS             7509..8777
                     /locus_tag="VNN25_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXH90030.1"
                     /translation="MRRALVVAALLATIALRARANVLRLNVNDTIQPISDEFIGRALD
                     QAQREHDDAVILVMSTPGGLLDSTRSIVEKIMTSRVPVIVFVAPAGSRAASAGFFILE
                     AADIAAMAPGTNTGAAHPVLFGEKMDEIMKVKMENDAAAFMRTIAAKRGRNVAVAESA
                     VRESKSFTEQEALAQKLIDVIAPNDRALLMAIDGRTLKRYDGTTTTLHVTNANINTVQ
                     MSLRQKLMSALMDPNFAFLLLVLGGLAIFAEFNHPGAIAPGVVGVIAILLALFALNLL
                     PVRYASLALLAAAFVLFALEAKFASHGVLGAGGIVCMVIGGLFLVDGPIPQMRVNILT
                     ALAVSIPIGLIAVLLTTLVLRARHGRVSTGSDGMIGEIGVARTSIGADGKVFVHGELW
                     NAMAKSVIPEGSRVRVAGINGLHLMVEPAE"
     gene            8857..9609
                     /locus_tag="VNN25_00515"
     CDS             8857..9609
                     /locus_tag="VNN25_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXH90031.1"
                     /translation="MGLVVIIVILGLYLLTSIKILPEYERGVIFRLGRLLPEAKGPGI
                     FLVFAPIDRMVRVSLRQEAFEVPPQDIITRDNVTLKVNAVVFLRVIEPRNAVVEVFDY
                     RYQTSQFAQTTLRSVLGEVELDELLAHRDSLNARIQTILDQHTEPWGVKVANVEVKQV
                     DLPESMLRAMAKQAEAEREKRAKLIHAEGEFAASQRLADAAHVLATQPTALQLRYLQT
                     LTEIGVEKNTTIVFPLPIDILSGLTKFLNKQS"
     gene            9613..10176
                     /locus_tag="VNN25_00520"
     CDS             9613..10176
                     /locus_tag="VNN25_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90032.1"
                     /translation="MSVVMIEAAVFQLRAAADEIVPGLAPQLGLCVNVLARAVESARD
                     EVNAARVSDIEFAVNDLAAAVDELPPSDAGRIQPLLDAIRGDLDALKTATSLDPKLIE
                     QINAFQSKLRERMKAIERQTYVEGGTDAPLPHPPASLRHDAIPLSHELAAAGFATPAL
                     DTLINKHDELRYHSIREILDELEVILG"
     gene            10269..10688
                     /locus_tag="VNN25_00525"
     CDS             10269..10688
                     /locus_tag="VNN25_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HXH90033.1"
                     /translation="MNALFALALLQETTTTDAVPVQTMSPAVGMVIGICYLVVILLIV
                     ISMWKIFVKAGKPGWAAIVPIYNLIVILEIAGKPIWWFILLLIPFVNIIVLIMVLISF
                     ARNFGKGVGFAIGMLILPFIFYPMLAFGDATYQPVAA"
     gene            complement(10728..11192)
                     /locus_tag="VNN25_00530"
     CDS             complement(10728..11192)
                     /locus_tag="VNN25_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXH90034.1"
                     /translation="MKTAAYEECYQSIVAIWRDEGGTTLDDIALMATINSILAHRFPH
                     FYWTGFYRVCGDRLVVGPYIGTVGCLQIEFGRGVCGTAAAERKTIIVPDVNAFPGHIA
                     CDPNSKSEIVVPVFAPDGVLVGVLDVDSDQLDAFDEEDQRGLEKIVALFGQT"
     gene            complement(11189..12655)
                     /gene="glgA"
                     /locus_tag="VNN25_00535"
     CDS             complement(11189..12655)
                     /gene="glgA"
                     /locus_tag="VNN25_00535"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HXH90035.1"
                     /translation="MKILFATAEVSPIAKTGGLGDVCGSLPKALANLGHDVTIFMPYY
                     REAHEWFANNGVTLDEAVPPMYINWGNWAAEATYLRTTLPRTDIPLVLVANDYFFNRP
                     HIYSPRLDGYDDGIERYSFFCRAVIRGCELLGIAPDVVHAHDWHTALLPIYLHSGLRG
                     SENFRGARSVYTIHNLNYQGIGTRRQFATLGLHSRYWAPDAVEHFGDVNLMKGGIIFA
                     NQVTTVSPTYAKEIQTHEMGAGLDGILRSLSFKLHGILNGIDVHEWDPADDRHIASPF
                     HLGHLAGKSLSKRALLREFKLTHKSKTPLLGFISRLTDQKGIDLLIAALPQMLAAGAQ
                     AIILGSGEPRFENALVHIAAQHPESCRVIIGFDNALAHRIYAGADILLMPSIYEPCGL
                     NQMYALRYGTVPVVRLTGGLADTVIPFHGANLDSANGFGFITINPGDFFTATWLAMLN
                     HRETKAWRQLQDNGMAADLSWERSAREYETIYHRAQFS"
     gene            complement(12726..13541)
                     /gene="amrB"
                     /locus_tag="VNN25_00540"
     CDS             complement(12726..13541)
                     /gene="amrB"
                     /locus_tag="VNN25_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXH90036.1"
                     /translation="MATRQPAVAGTFYEGDPDRLAAQVRACFAENGDVEGGERFIGAV
                     VPHAGLMYSGHVAAAFYGQAELPRRFVILCPNHTGFGHFAAINRQGSWRTPLGDVSID
                     TPLADALMQQSALLAEDDRAHAREHSLEVQLPFLQQLLGDFTFVPICLGANRYDYCEE
                     IGSAIAAVIAVAGEPVGIVASSDLNHYEDQKTTLRKDQLAIDEVLKLDPRGLWRAVEE
                     SDISMCGFIPTTAMMIAATKLGAKHARLIRHATSGDVNGDYSHVVGYAAIAIS"
     gene            13604..15046
                     /locus_tag="VNN25_00545"
     CDS             13604..15046
                     /locus_tag="VNN25_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HXH90037.1"
                     /translation="MAEIRREGERRAKLKSRFATARTVVWSWLTDPAVLLYRREAHGR
                     KRARVTTVEFFLFLREVTREFYDIEGTSRAAALAYTTLLSLIPLLVAFTTTLQRYFRT
                     VFPNFQSQIDNILNNIIPYQSPVISYHIAKFAETAQAASTFGVIIFIVITFRLFLAVE
                     TTVNQIWKVRNARGYRQKIIAFTMLFFWGPVLMGISFTTTNTLEKNRYLRVLFRQDII
                     FHIAPIIVLFIAFTMLFWLVPSTRVRFAAAAVGALVTTSLFTLVRFGFGIYADHLFHG
                     RFNLIYGALGLAVIFLIAIDILWVVILLGVEISYVYQNLYGLLRANEQQVQDEPRFDL
                     YFALRALIEISRRFDRREEAPSSYRLAEQFGTTDSQMLRVLGRLGEAQLIKETGGDWA
                     GFVPGCDPDRITIEEIVAQIEGLRREMPEIHMDDPARVAIGDLFARLTTSLSNALDHR
                     TIGQLVRELYGQRAPSRQHDERVEDPLPEA"
     gene            complement(15464..16306)
                     /locus_tag="VNN25_00550"
     CDS             complement(15464..16306)
                     /locus_tag="VNN25_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH90038.1"
                     /translation="MLRVRSMLFVIFAAVFLVLWAVVYATLPAVRHLGQMLARLLARS
                     AWATKWVSSTRDRFKNYLPVAVILIAGALLTAWAGDGFIDLAENVRAKSSTLIQTDAR
                     IHDWSVTERSPGATTFFTVMTIIGGPVSVAAIVAVIAVILAIGRRWRWLIYLLVTAGG
                     GGLLNLELKRYFARARPDVAEMLRRANGYSFPSGHAMGSAVAFGALAYLAFRAIRSWP
                     AKTAVMAFLYTLLAAIALSRVYLGVHWISDVLAGVTAGTVWVTTTTVAYETLRRIRRA
                     RGVG"
     gene            16629..17228
                     /locus_tag="VNN25_00555"
     CDS             16629..17228
                     /locus_tag="VNN25_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH90039.1"
                     /translation="MIPTNDSPEPDDRQLVEQVRHGDEGAFETLVRRKTSKVYGLCYR
                     VIGNSEDAKDISQLVFIKLWENIEKYDAQYAFDTWLYRMVTNVAIDFMRNKHSRDNAV
                     NSTLRLVKTSVDPEQTVTVQRKEVEAVFNAVSDVLSPKQKTIFVMSEMEDLRSSEIAK
                     ILGCQESTVRNHLFNARKVMQQQLKRRFPEYARLWEETR"
     gene            17225..17749
                     /locus_tag="VNN25_00560"
     CDS             17225..17749
                     /locus_tag="VNN25_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90040.1"
                     /translation="MITCRNFREKLQPGTSEPELLDHLRHCDACLDFAMSVDPDLFFR
                     SIGGEEIVPPGGVDAFVGDVMSQVRVRQAETSLAPHRTVSVWQRLAIAATVAAAVTGA
                     TLAYRFEHRPVTHPASAMSIAARTPATHFAPTATKSFVETYSSHNATIVEMPNDGSDT
                     KVVMIFDDKLPADL"
     gene            17746..18453
                     /locus_tag="VNN25_00565"
     CDS             17746..18453
                     /locus_tag="VNN25_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXH90041.1"
                     /translation="MNFKRVLLMISMTLSLCGAALAETVGEPAAATDAKPALSVRSFT
                     FKFKQADKAAAAIKPLLSAEGSMSIQPSSNALVVTDHAENMKAVAKMIGDFDKEPQSF
                     RLYVRIVGASRVEGASKIPDDLKDVARKLSMLPYNTYESVGEATVQGKEGDPGLIDMT
                     TGYRAGFHFGEYDPASDSIAVKDLQISKLTGAQKDQLTSLLKTSLNLTIGQTYILGAS
                     KAPQSQRALMIVLVARR"
     gene            18606..20003
                     /locus_tag="VNN25_00570"
     CDS             18606..20003
                     /locus_tag="VNN25_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HXH90042.1"
                     /translation="MRRFAFVAAVCILIALPLAAQNTDIESLSGLQFNFGNPGARSLG
                     MGGAFLGLADDASAAEANPAGLTILRKPEITIEGRNYQEQQVFTTSGTFPDLVRTGFS
                     HYSQRIDPTFASIVYPIKHFTFGVYYHEPLRNEGTGQVVPVRNSFTGEIKTDVPNFFL
                     PVDSNGNATGGPITSAACQALRQTNPFACLEYTVLPFLSSVKIQEKTFGLAVAYKIGN
                     FSIGATARSQRFNETAFTFRVTPTGDFSSISVQATSDIRKNNDVAKDESDITFAGGFK
                     WAPNDKFSIGGVYKQGAKFVAPTFAANENTNFAFVKAADTTFHIPDVYGAGVSVRPIP
                     VLTINADVVRVKYSNLVDNFVSINANVRAIDKAYAADDATELHLGGEYFFSTKIPFAV
                     RGGYWHDPQHSITYQGPLKNADEVAAAVLFPKTKSLNHVSVGAGLAWPRFQIDAAYDR
                     SQLFRVGSISMVTRF"
     gene            complement(20208..20765)
                     /locus_tag="VNN25_00575"
     CDS             complement(20208..20765)
                     /locus_tag="VNN25_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXH90043.1"
                     /translation="MKKLLAVLLVFAACATTHPPVVRDSGEYNRLVAADPDKRLVEIT
                     DIPRDVRYATTNNFMHAQLYLVARVYLRRAAAIALHEVERELAREGLGLKIFDGYRPY
                     RVTVRMWKPIRNPDFVADPAKGSRHNRGTAVDLTLIDRRTGHELPMPTGYDDFTSRAR
                     QDFKDLSPEVIANRAKLRRDGATRL"
     gene            complement(20762..21952)
                     /locus_tag="VNN25_00580"
     CDS             complement(20762..21952)
                     /locus_tag="VNN25_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003963148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXH90044.1"
                     /translation="MELDIFFSISQTEVDGYLPSERVMFENFFEQVVLADRLRFGTAW
                     IAESHLSTEVQKTNPGAVIPHFVGEIGLNTDILQLATRVFAKTKAIGVGSAIMNILCN
                     GGPIAAAERVKTFLALHGLDPEERRLLTIGFAAGRFPFINIPYGIVPRTSVEEAAWPV
                     VKSRIFDEATEIFLRLLRGETLNSSMITPHTLRRADFRSDADWQRVIDAYGQAVDEIP
                     IAPRWVFPNLKIVPQESRMDLLRLSIGSHDGATQIFANTILPVGVFNLSITPGDEIQK
                     TNERMRTAYHPDGGGWKRRLMPRTVLVFINDDPARAAEEARKALSNYWRALEGTLDEE
                     KVRRATGNALVGNAEMIAKQVAERFDPEDRLMLWFDFNNHDSKRVMKNMDDFMTKVAP
                     TIAR"
     gene            22067..23125
                     /locus_tag="VNN25_00585"
     CDS             22067..23125
                     /locus_tag="VNN25_00585"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HXH90045.1"
                     /translation="MNETSPINFARAMPNWQPLVEYRRNGVAENTIHGAVSWVSGRSV
                     VHSFGGNVEVYGRSMVKPIMMKVFTREFAKGLTDEQKAISVASHNGDTEHVRVARSIL
                     LQGEWGMMQAPLDVPLVQFGRQVRRPRRWYHCCSGEHAAILRGCKLKGWSRVGYVWPH
                     HPFFQEYLSYIQSVLGKDWKQGTIAKDGCGLPTVSMTVTDLARLFANLVTEKDEDWIW
                     RAMVEHPDLIGGFNRLDSTILKACNGRVIAKEGADGLLGLAIEHPEYPDGLGVVVKIA
                     HGWNPQATWYIARYILGVLGFEFRNPYKLRRQKAFIVPEVIPENLRERMTAIVPWDSW
                     DPDIDKWEFDPEELLATR"
     gene            23130..24536
                     /locus_tag="VNN25_00590"
     CDS             23130..24536
                     /locus_tag="VNN25_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXH90046.1"
                     /translation="MGGEFRAPVSGLYFDDVNPATTEVIAEVPDSDERDIDNAVRAAK
                     AAFPAWSKTPAAERSRLLLNLAVLVEQNLDELARMESIDNGKALSLAKKLDIPRAVLN
                     FRFFATAILHQASEAHVTDAAALNYTLRQPLGVVGLISPWNLPLYLLSWKIAPAIAAG
                     NTCIAKPSELTPLTANRLAELSIEAGIPPGVINIVHGYGAKAGRALTCHDDVAAISFT
                     GGTVTGAAVAANAAPRFKKISLELGGKNPNLIFADADLDDAVATSIRSSFWNQGEICL
                     CGSRIFVERSIHDVFVERLVAATKKLRIGDPLDEATDIGALISDAHLRKVMGYIDLAK
                     QEGGTIVTGGNRVDRPGYFLEPTIVTGLGCDCRVLQEEIFGPVVTITPFDDEEEAVRF
                     ANSTRYGLSATVWTRDLSRAHRVAAALDSGTVWINCWLLRDLRVPFGGMKESGLGREG
                     GFDSLMFFTEAKNVCVKI"
BASE COUNT         4741 a         7686 c         7347 g         4762 t
ORIGIN      
        1 cgtgctggcg gcatatcgcg cggcgtcgaa acacgcctac ctgccgaagc tgatggagca
       61 caacgacgcg gccatgctgc aacaggatgt cggcgatcaa acgctcttcg atctgctgca
      121 tcgcgaccgc gaggaaggcg tgggctggta cagaaaggcc gtcacgcttc tcgcttattt
      181 tcagaaggcc ggcgccgtcg acatcaaccc ccctttcacc ggcgacttct tcttcaacga
      241 gctggagatg tcgcgggagt tctacgtcga gaagctgatg cgtgtcagtc cggaatcggc
      301 acagccgctg aaaccgttgc tccgaaagct ggccgataac atctcacggc atccctatgt
      361 tctttgtcac cgtgattatc atggccagaa tattcatatc tttaacgaca agctttatct
      421 gatcgactat caggatctca gaatgggtcc ggacacgtac gacatcgcct cgctgctccg
      481 cgaccgcggc gtggcccgca tcctcggcga agagacggag ctcgagctcg tcgactacta
      541 tgcagagtgg cgcgtgcatg gtttgccggc acgctccgag cttccgcagg gcgaacgccg
      601 atcgatgcgc cgccgctatt tcgagaccct cctgcagcgc tccatcaaga ttctcggcac
      661 gttctctaaa cagccgctca cgcgcggccg catgtggtac ctcgacttca tcccgccgac
      721 gctggagagc gtccgtcgct gcatcgggga gttgcccgaa tacgggccgc tcgccgcgtt
      781 gcttccgatg gacttttcat tgtccgaggc gagagagcga gcggaaagga ttcatcatgg
      841 ttcgacataa aattactctt cttccaggcg acggaatcgg ccctgaggtc actgcctcag
      901 tcgtttcgat catcgaatgt gccggcgtcg atgtggagtg ggagaagttc ttcgtcggcg
      961 cggaagcgct ctcccgcttc ggcgatccgc tgccaggcga cgtgctcgaa tcgatcctgc
     1021 gcaacaaagt ggcgctcaag gggccggtga ccaccccgat cgggaccggc ttttcatcga
     1081 tcaacgtccg gctgcgaaag actctcgacc tctacgccaa tctgcggccg gtgaagtcga
     1141 tgccgaacat catcacgcgc tacgagaacg tcgacatcat cgtcgtccgc gaaaacacgg
     1201 aggatctcta cagcggtctc gagcacgagg tcgttcccgg cgtcgtcgag tcgatcaaga
     1261 tcatcaccga gcgcgcctcc acgcgcatcg ccagattcgc cttcgagtac gcacgcacgc
     1321 acggccggaa gcgcgtcacg gcggtgcaca aggcgaacat catgaagctc tccgacggcc
     1381 tgttcctgca gtgcttccga aacgttgcca aggagtatcc gaacatcgag gcggacgaca
     1441 agatcgtcga caacctctgc atgcagatgg tcatgaaccc gattcagttc gacgtgctgt
     1501 tgctcgaaaa cctttacggc gacatcgtct ccgatctcgc cgcaggcctg gtcggcggac
     1561 tcggcgtcgt ggccggcgcg aacatcggcg agaacggcgc agtgttcgaa gcggtgcacg
     1621 gaagtgctcc cgatatcgct ggtcaaaaca aggccaatcc gctggcactt ctgcagtcgg
     1681 ctgtgcagat gctgcatcac atcggcgaat cgggcgcggc gaagcgcgtc gatgacgcga
     1741 tcacgaaagt gctcgactcg ggaccggagc accggacacg cgatatcggg ggcattggta
     1801 ctaccgccga cttcacgaca gcgatgtgcg agacgttgcg ccgagggacg agggccggag
     1861 attgaggtag cgtggtgggt gttcttccgc gagaccgcga cgcctcgcga ccacgcaacc
     1921 gcatgatcga cttcgaaaca cacttccgcg ccggtctcga tcatttcaat gccggcgagt
     1981 tctggaatgc gcacgaagcg tgggagacga tctggctcga ggcggagtcg gaagtacacg
     2041 tgtttctgca gggcctcatc cagcttgcgg cggcctatca ccacgtcaaa cggggcactt
     2101 acccgggtgg gctgcggctt ttcgatgcgg cgttgcagaa gctggagggt tttccgaagc
     2161 gctggtgcgg gatcgatcgg acctcggctg agctggcagc gcaccgtcat cgggagtggg
     2221 tggcggatgt tttggcacgt aatgcgaaag ccgagcgact tgatgccaac gactttccgc
     2281 ggctcgttcc ggatgattct ccgattccac cgtttgaaca ctggtgatgc agcgtccgat
     2341 ctcgtatgat tcagccatcc ccaattccca acgccaccaa cgtagccgag gatccaaatg
     2401 caggaaatcg atcaacggga ccgtgagctg ctgggtgccc tgcagagtga gattcccctg
     2461 gtctcgacgc cgttcgctct catcggacaa gcgatcgaca tgtcggaaaa agaagtcatc
     2521 aagcgcatcg agcgcctgaa gcgcgaagga ctgctgcggc agctcgctgc gcaatttgat
     2581 gcccgagccc tcggctaccg ctcgtgcctc gtggcggcga aagtcgatcc tgaaagaatc
     2641 gacgacgcgg cagcgatgat caacgcgcat cccggcgtca cgcagaacta ccggcgcaac
     2701 aacgacttca atctctggtt cacgatcgcg gtcttatcgt cgtcgtcgct cggtctcaac
     2761 cgcacgatcg agatccttgg cagcgaggcc ggttgcgaga ccgtgcgcgc gctgccgacg
     2821 ctccggcagt tcaagggcaa cagcgacacc gatgaatcgc agagcgacac gcagttcgca
     2881 ccgctgacgt ccgtggagat cgagtccgtc cgcctccttc agcgcgacct gccactccag
     2941 ccgcgtcctt tcgaagcgat ggcgaagacg aacggaatcg gcgcggacga actactcgcc
     3001 gccgcgaagt cgctgctgca gcgcgggtac atccgccgct acagcgccgt cgtgcagaca
     3061 cgaaagaccg gattcggcgc cagcgcgatg ggcgtgtgga tcgtcccatc cgacgctgtc
     3121 gaagataccg gcacgaagat gtcgcagaac aaggccgtgt cgcattgcta cctccgcccc
     3181 gtgtacgacg actggccgta caacctctac acgatcgtgc acggccgttc tgtggacgag
     3241 tgcgaatcga tcatcaacga cctcgccatc gataccggcg tcgagtcgag gcaggctctc
     3301 tacccgaccc gcgagtacaa gaaagcgcgc atcaacctct tctctccgga agtggaagag
     3361 tgggagacgg cgcacggggg aagtcagagc gcgagcgcgg cgtcgtagat ctgatcggag
     3421 cgcctgttgc cgtcggtcgt aatcgccgaa atgactgaca tcccggagct cacgagcgcg
     3481 cagttttcca aggcgatccc ggcacgcgtt cggaaacgat tgatgcgggg tgaattcaag
     3541 tcaggcggag acgtgacggc gctcaggaga ttcctcggcc ttacgcaggt ggagctcgcc
     3601 cgagcgatga gcatcagtgt gtacacgctt cgcggttggg agcaaggcag acgtagtccg
     3661 gagggtcctg cgatcgcgct gctacgcatt gctgcccgcc atccgcgcgt cattcgcgaa
     3721 aacctgcggt cggctgcgta actccttctg gtcggagccc aggcaaaacg cccgcgttgc
     3781 cgcgggcgtt tctgtctgca catgagggcg tctacttgat gttctcggga aacacgctga
     3841 tgcggctgtt gccgcgcagc tcgttggtca gcttcgtcac ggtcgtgacg acgtcggacg
     3901 ccttctgcgt gatgaggaac tcgcgaatgc tggacttcac ggattcgaac ggttcggtgc
     3961 cgggagcctg acgtccttcg acaaagatca gatgaaatcc gaatttcgtc tggatgatgc
     4021 cgctgactgt gccgggcttc atgctccacg cagcagtctc gaagtcgggg tcggtctggc
     4081 ccttcgtgat ccatcccaga tcgcctccgc tctcccctga cgcatcctcg gagtacttct
     4141 tcgcagcatc cgcgaaatga gcgaggcgca atcgggccag aacctccgga ttcgacgtgc
     4201 tctgtgccga ggccacatct tcgcttcgaa gatcgacggc aatcttcttg atgcgatcga
     4261 gagcctcttc ctttgttttg cgcgcaggac caacctcggt cacgccgatc acgatgtgcc
     4321 tcacgtgaac tttctccggc gttgcaaagt cgtcaggatg ctcggtgtag tacgtcttga
     4381 cgtcgttatc cgtcacgatc tgtgcagcca cgacgtcgcg cacgtagcgg tcgaagagcg
     4441 ctgcttcgcg agcggcatcg accgcctcga taacatccgg ccgtttgttg taaccggact
     4501 tgatggcctc ctggatgaga aggcgcttgc ggatgtagtt ctcgagcagc gcgcccttgc
     4561 ctccgttggc atcgtagttc tggcgcatct gcgtgttgag attgctgtac atgcggtcga
     4621 gcttctcggc cgtgacagtc tcaccattga tgacggcgac cggcttcttg gaatcgggga
     4681 cagccaccgc cgccgattgc gccgcgggtg gctgcgccgg cgcactttgc tgtgcggccg
     4741 cgggcagggc cagcagcgag atgaaaagca tgcgtttgat caaagcgaat cctccagact
     4801 gattcagacg acgcggtgac caacagggtt tcaggaggag ggcattccga ggtgctagcg
     4861 aaatggaggc gcgaggtaag gagtgagcca gtctccgacc aaatgcatga aatcggcgaa
     4921 cgtcaggcta aggatgtcga gatcgaatcg ctcgcagatc gagggcagct ccttccgaaa
     4981 gctggcatac tttgattgca tcgccttcgc gccgggaatg ttcgtgcgac cggtttcagc
     5041 gcgaagccaa tcgcgaacac gtgcgatggc gagattcgga tcatcttcgt gattggcgat
     5101 gtcctgcccg gcaatgtcgg aaatgtaggt ctgaaatcga aaccggtcgc ggtcgaaaat
     5161 caggaaactc ttatcttctt gatcgtcgcc gtaagcacgg cagccgatgt cgattccgag
     5221 ctcgagcggc atgttgaaac gcggcagggc gctcgctcga tcgagctccg tgcgtgacag
     5281 atcatggatt gagtagcggc agtcgtcgat gatctcgagt atcttttcga gccgcacttc
     5341 gctcgaattt aacctcgacc gggcgcacac ggtccggaaa ccgcaagcct gaatcgtgaa
     5401 gacgaccgcc tcgaagattg gcgtgtattc gtcgtcgaat gggcagttga tgaagacgtt
     5461 tctctcgtac tcaggatgac gacttcgccg cacctcgaaa tcctcacttg cgcagagcgc
     5521 gaactgcttc gacggcttct cgaattcgct ccgctcccaa cgtgcttttc tccagcggga
     5581 agaggatgtc cggaccgtcg aggatcgaaa gcatgtgagg agctttcgtg atcaggtcgc
     5641 gaacttcctt cgtgttcgga agttctgcga actgatacag ctttccgagt ccgagagcct
     5701 tgagtgtttc tcgaacagtg cgagggctgc cagtcgcact tcggatctgc ttcagccgaa
     5761 tcgtcttccc tcggagtcgt ctcagacgga acttcttcgg tttcgttgcg tcggccatac
     5821 ctgatgataa acccgccggg tcgatcatga ttccgattct acgagacgta gaaaccagca
     5881 acttcctgtc aagagaaatc ttcacttttc aacagaccga atcaccgctc tgctaacctg
     5941 cgctcacctc cccggtcgat ggcgtttgcc gtcctgtgga aaaggtgtgg agggactgtc
     6001 tagcgattgt gggcatcgcg gcgcaacgac aaaccagccc tacactgcgc gtcccaaagt
     6061 ccaatgtctg ttgctcaact cgacgtcgcg ctcgaccggc cgatgccgca gtctcccgat
     6121 gcggagcgtg cggttctcgg atcgattctg atcaacaacc atgcgttcta tcgcgtggtg
     6181 ggcacgatca acaccgaaga ttttttccgc gatgcgcacc gcacgatctt cgccacgatg
     6241 cggcgtcttg ccgagcagtc gcgcgagatc gatccgctca cgctgcgcga ggagttgtcg
     6301 aagaacgcgc agctcgaaca ggtcggcggc tcggcatatg tcgcatcgct cgtcgacggc
     6361 atcccggaca ttgccaacgt cgagcgttac gcccgcatcg tcaaagagaa gtcgacgttg
     6421 cggcggctga tcgtgatggg caactcggtg atgcgcgccg cgctcgatgc accgagcgag
     6481 cctgcggacg tgctcaacat cgccgagaag tcgatctacg agattgccga gggatcgatc
     6541 gagaaggggt ttgtggcgct ggatcggatc acgcggacca acatgaccgc gatcgagcaa
     6601 ctgcagcacg ccggcaagct gctcaccggc atccccaccg gctacgaccg cttcaacgaa
     6661 ttcacctcgg gttttcagaa ccaggatttg atcatcatcg cggcaagacc gagcatgggg
     6721 aaaaccagtt ttatgatgaa catcgccgaa tcgatcgcga tccccgagcg atcgggtggt
     6781 ccgcggcctc cggcgcagcg tctttattcg gtcggcgtct tctcgctcga aatgtcgaaa
     6841 gagcagatcg gcctgcgcat tctctcgtcg gagtcgggcg tgtcgaacca tctcattcgt
     6901 gccggcatgc tgagtgagcg caactggcgc gatctcgccg atgcctcggc gcgcctggcc
     6961 aaggcaaaga tcttcatcga cgatacgccg ggcatggaca tcatggaaat gcgggccaaa
     7021 gcgcgccgcc tcaagatgga agccggtctc gacatgatca tggtcgacta cctccagctc
     7081 atggcggtga aagggaagac cgagtcgcgt aaccaagaaa tctcgcagat ctcgcgcggg
     7141 ctgaaggcca tcgccaaaga actgaacgtt ccgctcatct cgctctcgca gctttcgcgc
     7201 cgtcccgaac aacgcaccgg cgatcatcgt cctcagcttt ccgatcttcg cgaatcagga
     7261 tcgatcgagc aggatgccga cgtggtctgc ttcatctacc gcgacgaggt ttacaacaag
     7321 gacacggaag agaagggcat cgccgagatc atcatcggca agcagcgcaa cggcccgatc
     7381 ggcgacttca agctcgtctt ccgaaacgac atcacgaagt tcttcaacta cgagccgacg
     7441 ccggactacg gaccgcaata ggcgtagttc cgtatcccgc taccgtcagg aagagtacga
     7501 tcagcgctat gagacgcgcc cttgttgttg ctgctctcct cgccaccatt gcccttcgcg
     7561 ctcgcgcgaa cgtcctgcgg ctcaacgtca acgacacgat ccagccgatc agcgatgaat
     7621 tcatcgggcg tgcgctcgat caggcgcagc gggaacacga cgatgcggtg atcctagtca
     7681 tgagcacgcc gggcggactc ctcgactcga cgcgctcgat cgtcgagaag atcatgacct
     7741 cgcgcgtgcc ggtgatcgtc ttcgtcgctc cggcaggcag ccgcgccgcc tcggcgggat
     7801 tcttcatcct cgaagctgcg gacatcgccg caatggctcc cggaaccaac acaggcgcgg
     7861 cccatccggt gctgttcggc gaaaagatgg acgagatcat gaaggtgaag atggagaacg
     7921 atgccgccgc cttcatgcgc acgattgcgg cgaagcgcgg ccggaatgtc gcggtcgccg
     7981 agagcgccgt gcgcgagtcg aagtcgttta ccgaacagga agcgctggcg cagaagctga
     8041 tcgatgtgat cgcgcccaac gaccgggcgc tgctcatggc gatcgatggc cgcacgctca
     8101 aacgctacga cgggacgacc acaacactcc acgtcacgaa tgcaaacatc aacacggtgc
     8161 agatgtcgtt gcggcaaaag ctgatgtcgg cgctgatgga tccgaacttc gcgttcctcc
     8221 tgctggtcct gggaggactg gcgatcttcg ccgagttcaa ccatcccgga gcgattgcgc
     8281 ccggcgtcgt cggcgtcatc gcgatcctgc tggcgctgtt cgcgctcaac ctgctgcccg
     8341 tccgttacgc gtctctggcg cttctggccg cggcctttgt gctcttcgcg ctcgaggcga
     8401 agttcgccag ccatggcgtt ctcggtgccg gcggcatcgt ctgcatggtc attggcggac
     8461 tctttctcgt cgacggaccc attccgcaga tgcgcgtgaa tatcctcacc gcactcgcag
     8521 tcagcatccc cattggcctg atcgccgtgt tgttgacgac gctcgtcttg cgtgcgcggc
     8581 acggccgcgt ctcgaccggt agcgacggga tgatcggcga gatcggcgtg gcccgcactt
     8641 caatcggagc agacgggaaa gtcttcgtcc acggcgagtt gtggaacgcc atggcgaaga
     8701 gcgtgatccc ggaaggctcg cgcgtgcgcg tggccgggat caacgggctg catctaatgg
     8761 tcgagccggc ggagtgagct ttgcggtcgc cagaccgcga gaccacgccg ccaccgcgaa
     8821 accgatacaa tcgccagcgt ccgcgcggag gttcttatgg gtctcgttgt catcatcgtc
     8881 attctcggcc tttacctgct cacatcgatc aagatcctcc ccgagtacga gcgaggcgtc
     8941 atcttccggc tgggacgttt gctgcccgag gccaaggggc ctgggatctt tctcgtgttc
     9001 gcgccgatcg accgcatggt gcgcgtctcg ctgcggcagg aagcattcga agttcctccc
     9061 caggacatca tcacgcggga caacgtcacg ctgaaggtga acgcggtcgt ctttctgcgc
     9121 gtcatcgagc ctcggaacgc cgtggtcgaa gtgttcgact accgctacca gacctcgcag
     9181 ttcgcgcaaa cgacgcttcg ctccgtcctc ggcgaggtcg agctcgacga gctcctcgcg
     9241 caccgcgact cactcaacgc ccgcatccag acaattctcg atcagcacac cgagccgtgg
     9301 ggtgtgaagg tcgcgaacgt cgaagtgaag caggtcgatc tgccggagtc gatgctccgc
     9361 gccatggcca agcaggctga ggccgagcgc gagaagcggg cgaaactcat tcacgccgag
     9421 ggcgaatttg cggcgtcgca gagactggcg gatgccgcgc acgttctggc tacgcagccg
     9481 accgcgctcc agcttcgcta cctgcagaca ttgaccgaga ttggcgtcga gaagaacacg
     9541 accatcgtct tcccgttgcc gatcgacatc ctttcggggt tgaccaagtt cctgaacaaa
     9601 caatcataaa gaatgagcgt cgtcatgatc gaggccgcgg tctttcagtt gcgggccgcg
     9661 gcggacgaaa tcgttcccgg gctcgcgccg cagctcggtt tgtgcgtgaa cgtgctggcc
     9721 agggcggtcg agtctgcgcg cgatgaagta aacgccgcgc gcgtgagcga catcgagttc
     9781 gctgtcaacg atctcgccgc tgcggtcgat gagcttcctc catcggatgc tggccgaatc
     9841 cagccactgc tggatgccat tcgcggggac ttggatgcgc tcaagaccgc gacctctctc
     9901 gatccgaagc tcatcgagca gatcaatgcc tttcaatcga agctgcgcga gcggatgaaa
     9961 gcgatcgagc ggcagacata tgtcgaaggt ggaaccgatg ccccgctccc tcatccaccg
    10021 gcatcgctgc gccacgacgc catcccactt tctcacgagc tggccgccgc cggattcgcc
    10081 acaccggcgc tcgatacgct gatcaacaag cacgatgagc tgcgatacca ctcgattcgg
    10141 gaaatcctcg acgagctcga agtcatcctg ggctgaacga tttccttgat ccttgcgtca
    10201 gccgagtccg gtatactgtt tcaacaaatc ggtcacaact tgcgcttgga gaaggagaag
    10261 gagaagagat gaacgctctg ttcgcactcg cactactgca ggaaacgacc accaccgacg
    10321 ccgtccccgt gcagacgatg tcacccgccg tagggatggt catcggtatc tgctacctcg
    10381 tcgtcattct gttgatcgtc atttcgatgt ggaagatctt cgtcaaagca gggaagccgg
    10441 gttgggccgc gatcgtcccg atctataacc tgatcgtgat tctcgaaatc gccggcaagc
    10501 cgatttggtg gttcatcctg ctgctcatcc cattcgtcaa catcatcgtg ctgatcatgg
    10561 ttctcatatc cttcgccagg aacttcggga agggcgtcgg ctttgcgatc gggatgctga
    10621 tcctgccgtt catcttctat ccgatgctcg cgttcggcga cgcgacgtat caacctgtag
    10681 ccgcgtaaga ctcctccagg gcccaggctg cgagcgcggt ctgggcccta cgtttgtccg
    10741 aagagcgcga cgatcttttc gagaccgcgc tgatcttcct cgtcgaacgc gtcgagctga
    10801 tcagaatcga cgtcgagcac gccgacgagc acgccgtcag gcgcgaacac cggcacgacg
    10861 atctccgact tcgagttcgg atcgcaggcg atgtggccgg ggaatgcgtt cacgtccggc
    10921 acgatgatcg tcttgcgctc ggcagcggca gtgccgcaaa cgccgcgtcc aaactcgatc
    10981 tgcaggcaac ccaccgtgcc gatgtacggt ccgaccacga gacggtctcc gcaaacgcgg
    11041 tagaagcccg tccagtagaa gtgtgggaaa cgatgcgcga ggatcgagtt gatcgtcgcc
    11101 atgagcgcga tgtcatcgag cgtcgttcct ccctcatcgc gccagatggc aacgatcgac
    11161 tggtagcact cctcgtacgc ggcggtcttc atgagaactg ggcgcgatga tagatggtct
    11221 cgtactcgcg agccgagcgt tcccacgaaa ggtcggcggc catgccgttg tcctgcaact
    11281 gacgccacgc tttcgtttcg cgatggttga gcatcgccag ccaggtggct gtgaagaagt
    11341 ctcctgggtt gatcgtgatg aaaccaaagc cgttcgcgct gtcgaggttg gcgccatgga
    11401 acggaatcac ggtgtcggcc agaccgcctg tgagccggac gaccgggacc gtgccgtagc
    11461 gcagcgcgta catctgattc aggccgcacg gctcgtagat cgagggcatg agcaggatgt
    11521 cggcgccggc gtagatgcga tgcgcgagcg cgttgtcgaa tccgatgatg acgcggcacg
    11581 attcgggatg ctgcgctgcg atatgcacca gcgcattctc gaaccgcggc tcgcccgacc
    11641 cgaggatgat cgcctgtgcg ccggcagcga gcatctgcgg cagcgcggcg atcagcaggt
    11701 cgatgccctt ctgatcggtc agtcgcgaga tgaatccgag gagcggcgtt ttcgacttgt
    11761 gcgtcagctt gaattcgcgc aggagtgccc gtttcgagag actcttgcct gcaaggtgcc
    11821 cgaggtgaaa cggcgacgcg atgtgacggt cgtcagccgg atcccactca tgaacgtcga
    11881 tgccgttgag aatgccgtgc aacttgaagg agaggctgcg caggatgccg tcgagccccg
    11941 ctcccatctc atgtgtctgg atctccttcg cgtaagtggg cgatacggtc gtgacttggt
    12001 tcgcgaagat gatgccgccc ttcatcaggt tcacgtcgcc gaagtgttcg acggcgtcgg
    12061 gcgcccagta gcggctgtgc aagccgaggg tggcgaattg ccgtcgagtg ccgatgccct
    12121 ggtagttgag attgtggatt gtgtagaccg agcgtgcccc gcggaagttc tcgctgccgc
    12181 gcaatcctga gtgcaggtag atcggcagga gtgccgtatg ccagtcgtgg gcgtgaacca
    12241 cgtccggcgc gatgccgaga agctcacacc cgcgaatgac cgcgcggcaa aagaacgagt
    12301 agcgctcgat gccgtcgtcg tagccgtcga gccgagggga gtagatgtgt ggccggttga
    12361 agaagtagtc gttggcaacc aggacgagcg ggatatcggt acgcggcagc gtcgtccgca
    12421 gatacgtcgc ctcggccgcc caattccccc agttgatgta catcggcggc acggcttcat
    12481 cgagcgtgac gccgttgttg gcaaaccatt cgtgcgcttc gcggtagtac ggcatgaaga
    12541 tcgtgacgtc gtgtccgagg tttgcaagcg ctttcggcag cgagccgcag acatcgccca
    12601 acccgcccgt cttggcgatc ggcgaaacct cggcggtagc aaacagaatc ttcacgacgc
    12661 gactctatct cctgacagct tctatctgcg tcccatctgc gccatctgcg gatgaacggt
    12721 tccagctacg aaatagcgat cgccgcatac ccaacgacat gcgagtagtc gccattgacg
    12781 tcgccgctgg tggcgtgccg gatcagcctt gcgtgttttg cgccgagctt cgttgccgcg
    12841 atcatcatcg ccgtcgtcgg gatgaatccg cacatggaga tgtcggactc ctcgaccgcg
    12901 cgccagagcc cgcgtggatc gagcttcagc acctcgtcga tcgcaagctg gtctttgcgc
    12961 agcgtggttt tctggtcctc gtagtgattg aggtcggagc tggccacgat gccgaccggc
    13021 tcgccggcaa cggcgatcac tgccgcgatg gcacttccga tctcttcgca gtaatcgtaa
    13081 cgattcgcgc cgaggcagat ggggacgaac gtgaagtcgc cgaggagctg ctgaagaaac
    13141 ggaagttgca cctcgagcga atgctcacgc gcgtgggctc gatcgtcctc ggcaaggagc
    13201 gctgactgct gcatcagggc atcggcgagg ggtgtatcga tgctgacatc gccgagggga
    13261 gtgcgccatg atccctggcg attgatcgcg gcgaagtgcc cgaagccggt gtggttcggg
    13321 cagaggatga cgaagcggcg cggcagctcg gcctgcccgt aaaacgcggc cgccacgtgg
    13381 ccgctgtaca tcaaaccggc gtgaggaacg acggctccga tgaatctctc tcccccctcg
    13441 acgtcaccgt tttcggcaaa gcaggcgcgg acttgggcgg ccaggcggtc cgggtctccc
    13501 tcgtaaaagg tgcctgccac ggctggctgg cgggtagcca tgatccataa cgttatcgca
    13561 tctcctggcc gcaggctcac tccttgcctt tatagttgag gtaatggccg agatacggcg
    13621 cgagggagag cgtcgcgcga aactgaaatc gaggtttgcc accgcccgga cggtcgtctg
    13681 gtcctggctg acggacccgg cggttctgct ttaccggcgt gaagcgcacg gccggaagcg
    13741 ggcgcgcgtc acgacggtcg agttctttct cttcctccgc gaagtgaccc gcgagttcta
    13801 cgacatcgag ggaacgtcac gtgcggcggc gctcgcctac acaactcttt tgtcgctcat
    13861 cccgctactc gtcgccttta cgacgacctt gcaacgctac ttccggacgg tgtttcccaa
    13921 tttccagtcg cagatcgaca acatcctcaa caacatcatc ccgtaccagt cgccggtgat
    13981 ctcgtaccac atcgccaaat tcgcggagac ggcacaggct gcgtcgacgt tcggcgtgat
    14041 catcttcatc gtcatcacct tccgcctgtt cctggcggtc gagacgacgg tgaatcagat
    14101 ctggaaggtg cggaacgccc gcggctaccg ccagaaaatc atcgccttca cgatgctctt
    14161 tttctggggc ccggtgctga tgggcatctc gttcacgacg acgaacacgc tcgaaaagaa
    14221 ccgctatctc cgcgtgctgt ttcgccagga catcattttt cacatcgcgc cgatcatcgt
    14281 cctcttcatc gccttcacga tgctcttctg gctcgtgccg tcgacgcgcg tgcgcttcgc
    14341 cgcggccgct gtcggggcgc tcgtaacgac gtcgctcttc acgctggtcc gcttcggctt
    14401 cggcatctac gccgatcatc tgtttcacgg ccgcttcaat ctgatctacg gcgcgctcgg
    14461 gttggcggtc atcttcctga tcgcgatcga catcctctgg gtcgtgatcc tcctcggcgt
    14521 cgagatcagc tacgtctacc agaacctcta cggactgctg cgcgccaacg agcagcaggt
    14581 gcaggacgag ccgcgtttcg atctctactt cgcgctccgt gcactgatcg agatttcgcg
    14641 ccgcttcgac cgccgcgagg aggcgccatc gtcgtaccgg ctcgcggaac agttcgggac
    14701 gaccgattcg cagatgctgc gggtcctggg ccggctcggg gaagcgcaac tgatcaaaga
    14761 gacgggaggc gattgggccg gcttcgttcc gggctgcgat ccggaccgca tcactatcga
    14821 ggagatcgtc gcgcagatcg aaggtcttcg ccgcgagatg cctgagattc acatggacga
    14881 cccggcgcgg gttgccatag gcgacctttt cgcgcgcctg accacgtcgc tcagcaatgc
    14941 gctggatcac cggacgatcg gccagcttgt ccgcgagctg tacggccaaa gggcgccgtc
    15001 gcgtcagcac gatgagcgtg tggaagaccc gctgcccgaa gcctgacagg cctctctcaa
    15061 gttcacttcg gactcctacc ttcgcaacta atcctttgca aaagcgcctt ctgcggaaga
    15121 cgtgatgatt gcgatatcgg tccggcgcat tccgacatcg gtccaaattg gaccggcatc
    15181 gatccagatt ggaccggcat cggtccagtt tggagcgaca tcggtccagt ttgatccgac
    15241 atcggtccag tttgatccga catcggtcca atttgatccg tcatcggtcc ggtttgatcc
    15301 gtcatcggtc caatttgatc cgtcatcggt ccagtttgat ccgtgatcgg tccagtttga
    15361 tccgtcatcg atccaatttg gaccggcatc gatccgtttc tttccgactt attaccgcct
    15421 tttccggtct tttggccgct ctaaggccgc ttttggccct tttctaaccc acgccgcgcg
    15481 cgcgccggat tcttcgcagt gtttcgtagg cgacggtcgt ggtggtcacc cagaccgtcc
    15541 cagcggtgac gccggcgaga acgtccgata tccagtgaac gccgaggtag acgcgggaga
    15601 gggcgatggc agcgagtagc gtgtagagga acgccatcac cgccgttttc gcgggccagc
    15661 tccgaatggc gcggaacgcc agataggcca gcgctccaaa tgccaccgcc gagcccatgg
    15721 cgtggccgct cgggaacgaa tagccgttcg cgcggcgcag catctcggct acgtcggggc
    15781 gggctcgggc gaaatagcgc tttagctcca ggttcagcag accgccccca cccgccgtga
    15841 cgaggaggta gatcagccag cgccagcgcc ggccgattgc caggatgacg gcgatgaccg
    15901 cgacgatcgc ggcaacgctg accgggcctc cgatgatcgt catgacggtg aagaaggtgg
    15961 tagctccggg cgagcgctcg gtgacgctcc agtcgtggat ccgcgcatcg gtctgaatga
    16021 gtgtgctgct cttcgcgcgc acgttttcag cgagatcgat gaagccgtcg ccggcccacg
    16081 cggtgagtaa tgctccggcg atgagaatca ccgccaccgg caggtagttc ttgaagcggt
    16141 cgcgcgtgct cgaaacccac ttcgtcgccc acgcactgcg cgccaacaga cgggcaagca
    16201 tctgtcccag atgcctcacc gcggggagcg tggcatagac gaccgcccag agaaccagga
    16261 agaccgctgc aaagatgaca aacagcatgc tgcgcactct gagcatagac gatgcctgta
    16321 ctggggagtt gcgggtcggg agtcgggtgt cggagccagg ctggggagtc gcgggtcggg
    16381 tgtcgggagt cagagccagg ctggggagtc gcgggtcggg agtcgggtgt cggagccagg
    16441 caagatcgct ccgacgtccg ctccgacccc gacccccgac ccccgactcc cggccttcgc
    16501 gaaatcccag gctccgaccc ccgacccccg aaccgcgact cccgcatccg ccgaaatcct
    16561 ccagccccct tggacgttcc gtcctgcact gccgtacctg tgtcacgatg cctgcggaag
    16621 tactccgaat gatcccgaca aacgactctc cggagcccga cgaccgtcag ttggtcgaac
    16681 aggtccggca cggcgacgag ggcgcattcg agacgctcgt ccgccggaag acttccaagg
    16741 tctacggcct ctgttaccgc gtcatcggca acagcgagga tgccaaggac atctcccagc
    16801 tcgttttcat caagctgtgg gagaacatag agaagtacga cgcccagtac gccttcgaca
    16861 cctggctgta ccgcatggtg acgaacgtcg ccatcgactt catgcgcaac aagcactcgc
    16921 gcgacaacgc ggtcaactcg accctgcgcc tcgtcaagac ctcggtcgat ccggagcaga
    16981 cggttacggt gcagcgcaaa gaggtcgagg cggtcttcaa cgccgtctcg gacgtcctct
    17041 cgccgaagca gaagacgatc ttcgtcatga gcgagatgga agatctccgc tcctccgaga
    17101 tcgccaagat cctcggctgt caggagtcca ccgtccgcaa tcacctcttc aacgcccgca
    17161 aggtaatgca gcagcagttg aagcgtcgtt ttcctgaata cgcccggctg tgggaggaga
    17221 cgcgatgatc acctgccgga actttcgcga aaaactccag cccggaacca gcgaaccgga
    17281 gctgctcgat catcttcgtc actgcgatgc ctgtctcgac ttcgccatga gcgtcgatcc
    17341 tgacctcttc ttccgctcga tcggtggcga agagatcgtc ccgcccggcg gcgtcgatgc
    17401 cttcgtcggc gatgtcatgt cgcaggttcg cgtccgtcag gctgagacct ccctggcacc
    17461 gcatcgcacg gtcagcgtgt ggcagcggct tgccatcgcc gcgaccgtgg ccgctgctgt
    17521 caccggcgcg acgctcgcct atcgtttcga gcaccgcccg gtcacgcatc cggcgagtgc
    17581 catgtccatt gccgccagga cgccggcaac gcatttcgcc ccgaccgcga cgaaatcgtt
    17641 cgtcgaaacg tattcatccc ataacgcgac gattgtcgag atgccgaacg acggcagcga
    17701 caccaaagtc gtgatgatct tcgacgataa gttgccggcg gatttatgaa cttcaagcgt
    17761 gttcttctga tgatctcgat gacgctgtcc ctttgcgggg cagcgcttgc ggaaactgta
    17821 ggcgagcctg ccgcggcaac ggatgccaag cccgcgctgt cggtgcgttc gttcaccttc
    17881 aagttcaagc aggctgacaa agctgccgcg gcaatcaagc cgcttctgag tgccgagggc
    17941 tcgatgtcga tccaaccgtc gtcgaatgcg ctcgtcgtca ccgaccacgc cgagaatatg
    18001 aaagcggtcg ccaagatgat cggcgacttc gacaaggagc cgcagagctt tcggctttat
    18061 gtccgcatcg tcggcgcctc gcgcgtcgaa ggtgcgtcga aaatccctga cgacctcaaa
    18121 gatgtggcgc ggaagctgtc catgcttccg tacaacacct acgaaagcgt cggcgaagcg
    18181 accgtgcagg ggaaagaagg cgacccgggg ctcatcgaca tgaccacggg ctatcgggct
    18241 ggtttccact tcggtgaata cgacccggcc agcgactcga tcgcggtcaa agacctgcag
    18301 atctccaagc tcacaggcgc acaaaaggat cagttgacgt cgctgctcaa gacctcgctg
    18361 aacctgacta tcggccagac ttatattctc ggcgcatcga aggccccgca aagccagcgt
    18421 gcgttgatga tcgtcctcgt cgctcgacgg tagagcggcg gcaacctcct acacacgggc
    18481 gcggcggatt caccctcctg ccgcgccctt tttttcgtgg ttgcagggtt tgcgatcgcg
    18541 cgaccgcgaa cccgcgcgac gggctctgct atccttcgtc caattcgttc atcggaggat
    18601 tcccaatgcg cagattcgct ttcgtggccg cggtttgtat actgatcgcg ctcccccttg
    18661 ctgcacaaaa caccgacatc gaatcgctct ccggtctgca gttcaacttc ggcaatcccg
    18721 gagcgcggtc gctcgggatg ggcggcgcct ttctcggcct ggccgacgac gcctccgccg
    18781 cggaagccaa tccggcaggg ctgaccattc tccggaagcc cgagatcacg atcgaaggac
    18841 gcaactatca ggagcagcag gtcttcacga ccagcggcac gttccccgac ctcgtgcgca
    18901 ccggcttcag ccactacagc cagcgcatcg acccgacctt cgccagcatc gtctacccga
    18961 tcaaacactt caccttcggc gtctactacc acgagccgct gcggaacgaa ggaaccggcc
    19021 aggtcgtacc ggtgcgcaac agcttcaccg gcgagatcaa gaccgacgtc cccaacttct
    19081 tcctcccggt cgacagcaac ggaaacgcca ccggtggtcc gatcacatcc gccgcttgcc
    19141 aggcattgcg tcagacgaat ccgttcgcct gcctcgagta cacggtgctg ccgtttctca
    19201 gctccgtgaa gattcaggag aagacgttcg gtctcgccgt cgcttacaag atcggcaatt
    19261 tctccatcgg cgccacggca cgctcccagc gtttcaacga gaccgccttc acgttccgcg
    19321 tcacgccgac cggcgatttt tccagcatct ccgtgcaggc cacgagcgat atccgcaaga
    19381 acaacgacgt agcgaaggac gagagcgaca tcacgttcgc gggcggcttc aagtgggcgc
    19441 ccaatgacaa gttctccatt ggcggcgtct ataagcaggg ggcgaagttc gtcgcgccga
    19501 cgttcgcggc caacgagaac acgaacttcg cattcgtcaa ggccgcggat acaacgttcc
    19561 acatccccga cgtctacggt gccggcgtgt cggttcgccc gattccggtt ctgacgatca
    19621 atgccgacgt ggtgcgggtg aagtactcga acctggtcga caatttcgtc tcgatcaatg
    19681 cgaacgttcg cgccatcgac aaagcctacg ccgccgacga cgccaccgag cttcacctcg
    19741 gcggcgagta cttcttctcg acaaagatcc ccttcgccgt gcgcggcggc tactggcacg
    19801 atccacagca ctcaatcacc tatcagggtc cgctgaagaa cgccgacgaa gttgccgccg
    19861 ccgttctctt cccgaaaacc aaatcgctga accacgtctc cgtgggcgcc ggtctggcgt
    19921 ggccccgttt ccagatcgac gccgcctacg accgttcgca gttgttccgg gtgggctcga
    19981 tctcgatggt cacaagattc tagagctgcg cttcgcgcgt tatcactgtc ttagcgctgg
    20041 tttgacaacg cgcgaagcgc aatcaaccga caaccgacaa cgcgcaaagc gcaactatcc
    20101 gacaacgcgg ccaagtcgcc aatcacaatc gctctagcgg caaatcgagc aactcaaacc
    20161 tctcccatcc gcgaaaatca aagtgccacc actcggacgg caacggatca aagccgtgtc
    20221 gcaccatcgc gtcgcagctt tgcacgattg gcgatcacct cgggggacag atctttgaaa
    20281 tcctgccgcg cgcgcgaggt gaagtcgtcg tagccggtgg gcatgggtaa ctcgtggccg
    20341 gtgcgacgat cgatcagcgt cagatcgacc gcggtcccgc gattgtggcg tgagcctttg
    20401 gcgggatcgg cgacgaagtc aggattgcgg atcggcttcc acatccgaac ggtgacgcgg
    20461 tacggacgat agccatcgaa gatcttcagt ccgagtcctt cccgtgccag ctcgcgctcg
    20521 acctcgtgca gtgcgatcgc ggcagcccgg cgcaggtaga ccctcgccac cagatagagc
    20581 tgcgcgtgca tgaagttgtt cgtcgtggcg tagcgaacgt cgcgcggaat gtcggtgatc
    20641 tcgacgagac gcttgtcggg gtcggcggcg acaaggcggt tgtactcgcc ggagtcacgg
    20701 actaccggtg ggtgcgtggt cgcgcaggcg gcaaaaacga gcaggactgc cagcagcttc
    20761 ttcatcgtgc gatcgtcggt gcaaccttcg tcatgaaatc gtccatattc ttcatcacgc
    20821 gtttcgaatc gtgattgttg aaatcgaacc agagcatcaa ccggtcttcc ggatcaaacc
    20881 gctcggcgac ctgtttcgca atcatctccg cattgccgac gagcgcgttg cctgtcgcgc
    20941 gccgaacctt ctcttcgtcg agcgtccctt ccagcgcgcg ccagtagttc gacaacgcct
    21001 tccgcgcctc ctcggctgcg cgcgccggat cgtcgttgat gaaaaccagc accgtgcgcg
    21061 gcatcagacg gcgcttccag ccgccgccat cggggtgata agccgtgcgc atccgctcgt
    21121 tcgtcttctg aatctcgtcg cccggcgtga tcgagagatt gaacacaccg accgggagga
    21181 tggtgttcgc aaagatctgc gtcgctccgt cgtgcgatcc gatcgaaagc cggagcagat
    21241 ccatgcgcga ctcctgcggc acgatcttca gattcgggaa aacccatcgc ggcgcaatcg
    21301 ggatctcgtc aacggcctga ccgtaagcat caattacgcg ctgccagtcc gcgtcgctgc
    21361 gaaagtcggc gcgccgcaga gtatgagggg tgatcatcga tgagttgagc gtctcgccac
    21421 gcaagaggcg tagaaagatc tccgtcgcct cgtcgaagat cctgctcttc acgaccggcc
    21481 acgctgcttc ctcgaccgat gtgcgcggca cgatgccgta ggggatgttg atgaacggga
    21541 agcgccccgc ggcgaagccg atcgtcagca gtcgccgctc ctccggatcg agcccgtgaa
    21601 gcgccagaaa cgtcttcacg cgttccgccg ccgcaatcgg gccgccgttg cagaggatgt
    21661 tcatgatcgc cgagccaacg ccgatcgctt tcgtcttcgc aaaaacgcga gtggcgagct
    21721 gcaggatgtc ggtgttcaaa ccgatctcac ccacaaagtg cgggatcacc gcgccgggat
    21781 tggtcttctg gacttccgtg ctgagatggc tctccgcgat ccaagcggtc ccgaatcgca
    21841 accggtcggc gagcacgacc tgctcgaaga aattctcgaa catgacccgc tcgctcggca
    21901 gatacccatc gacctcggtc tgcgagatgg agaagaagat gtccagttcc atgcaggcct
    21961 gatcgtatag gtaatctgcg tccatctgcg taatctgcgg acaggttttt atccgcagat
    22021 tacgcagatg tactcagatg aaggcctcct ataatcgccg cgtgccatga acgagacttc
    22081 ccccatcaac ttcgctcgcg ccatgcccaa ttggcagccg ctggtcgagt accggcgcaa
    22141 cggtgtggcg gagaacacga ttcacggcgc ggtctcatgg gtttcgggca ggagcgtcgt
    22201 ccattcgttc ggcggcaacg tcgaggtcta cggacgctcg atggtcaagc cgatcatgat
    22261 gaaagtcttc acccgtgagt tcgccaaagg actcacggac gagcagaagg ccatctccgt
    22321 tgcctcgcac aacggcgaca ccgagcacgt gcgcgtcgct cgctcgatcc tgctgcaagg
    22381 cgagtggggc atgatgcagg cgccgctcga cgtcccgctc gtgcagttcg ggcggcaggt
    22441 gcggcggccg cgccgctggt accactgctg ttccggcgag cacgccgcca ttcttcgcgg
    22501 ctgcaaattg aagggctggt cgcgcgtcgg atacgtctgg ccgcatcacc cgttttttca
    22561 ggagtacctc agctacatcc agagcgtact cggaaaggat tggaagcagg gaacgatcgc
    22621 taaagacggt tgcggattgc cgaccgtttc gatgaccgtc accgatctcg cccgtctctt
    22681 cgccaatctc gtcacggaaa aggacgagga ctggatctgg cgcgcgatgg tcgagcatcc
    22741 cgatctcatc ggcggcttca atcgtctcga ctccacgatc ctcaaagcct gcaacggccg
    22801 cgtcatcgcc aaagaaggag ccgacggtct cctcggcctc gccatcgagc atcccgaata
    22861 tccggacggc ctcggcgtcg tggtcaagat cgcgcacggc tggaacccgc aagccacctg
    22921 gtacatcgcc cgctacatcc tcggcgtgct cggattcgag ttccgcaatc cctacaaact
    22981 gcggcggcag aaagcgttca tcgtccccga ggtcattcct gagaatctgc gcgaacggat
    23041 gaccgcgatc gtgccgtggg actcgtggga tcccgacatc gacaagtggg agttcgatcc
    23101 ggaggagctc cttgcgactc ggtaacttca tcggcggcga attccgtgcg cccgtttcgg
    23161 gcctttactt cgacgatgtg aatccggcca cgacggaggt catcgccgag gttccggaca
    23221 gcgacgagcg cgatatcgac aatgccgttc gcgcggccaa ggctgcgttt cccgcgtgga
    23281 gcaagacacc ggcggcggaa cgctcgcggc tcctgctcaa cctcgccgtt ctcgtcgagc
    23341 agaacctcga tgagctggcg cggatggagt cgatcgacaa cggcaaggcg ttgtcgcttg
    23401 cgaagaagct cgacatcccg cgggctgttc tgaatttccg cttcttcgcc accgccattc
    23461 ttcatcaagc cagcgaagcg catgtcaccg acgcggctgc gctcaactac acactgcggc
    23521 aaccgctcgg cgtagtcggt ctgatctcgc cgtggaacct gccgctgtac ctgctgtcgt
    23581 ggaagatcgc gcccgcgatt gcagccggga acacgtgcat cgcgaaaccg agcgagctga
    23641 cgccgctgac cgcgaatcga ctggccgaac tgtccattga agccggcatc ccgccgggag
    23701 tgatcaatat cgtccacggc tacggcgcga aagccggccg cgccctgacc tgccacgacg
    23761 acgttgccgc gatctcgttc accggcggaa cggtgaccgg agccgcagtc gctgccaacg
    23821 ccgcgccgcg cttcaagaag atctcactcg agctcggcgg taagaatcca aacctgatct
    23881 tcgccgacgc cgatctcgat gacgctgtgg ccacgtccat ccgctccagc ttctggaacc
    23941 agggcgagat ctgcctctgc ggttcacgca tcttcgtcga acgctcgatc catgatgttt
    24001 tcgtcgagcg cctcgtcgcc gctacgaaga agctgcgcat cggcgatccg ctcgatgagg
    24061 ccaccgacat cggcgcgctc atcagcgacg cccatcttcg caaggtgatg ggctacatcg
    24121 atctggccaa acaggaaggc ggcaccatcg tcaccggcgg gaatcgcgtc gatcgtcccg
    24181 gttacttcct cgaaccgacc atcgttaccg gccttggatg cgattgccgc gtcctgcagg
    24241 aagagatctt cggtccggtc gtcacgatca cgccgttcga cgacgaagag gaggcggtcc
    24301 gcttcgccaa ctccacccgc tacggcctct ctgccaccgt ctggacccgc gacctctccc
    24361 gcgcccaccg agtcgccgcc gcgctcgaca gcggcaccgt ctggatcaac tgctggctcc
    24421 tccgcgacct ccgcgtcccc ttcggcggca tgaaggaaag tggcctggga agagagggcg
    24481 gcttcgactc gctgatgttc ttcacggaag cgaagaacgt gtgcgtgaag atttag
//
LOCUS       DATLVW010000018         6418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_125121, whole genome shotgun sequence.
ACCESSION   DATLVW010000018 DATLVW010000000
VERSION     DATLVW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125121"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(11..96)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(188..811)
                     /locus_tag="VNN25_00595"
     CDS             complement(188..811)
                     /locus_tag="VNN25_00595"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH90047.1"
                     /translation="MAASAAAGDFSARDVPSAHIRQTGRVVIRNDARGVTAYHFFGDI
                     RQALFTLRSGPRAMHYTPFGTALFDSGARVTAPMPAYLSALIADASKQHGVDPRLVAA
                     IAARESAYNPGAVSPAGAVGVMQLMPATARYTGVRNAFDARENVFGGVHYLRTLLDTF
                     HGDLDLTLAAYNAGPGAVQRYNGVPPFRETKDYVRTVRAAYEMALKR"
     gene            897..2576
                     /locus_tag="VNN25_00600"
     CDS             897..2576
                     /locus_tag="VNN25_00600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXH90048.1"
                     /translation="MTIVRRAAALLFLAFTACTMQSPSNDNSVKRSNETIFEGGTVLA
                     GPSQTPQANWSVVTANGTIVAAGPADAIRAAHPNARVVDVHGTTVMPGLTDAHGHLYG
                     LGLSLDTVNVIGTTSYDEVIAKVRDRAQRASAGDWILGRGWDQNHWPGKQFPTAAPLD
                     AAISDHPVWIRRVDGHAGIANSAAMRAAGVTAATPDPEGGRVLRDAAGNPTGVFIDGA
                     MSLIDSKVPAPSPELRKARVLAAAQAIAENGLTEMHDAGAEQATITAIQQLIDEHKFP
                     IRVYLMLTDEPALLESWFARKPLIGYGGRLTVRSVKMYADGALGSRGAALLAPYSDDP
                     SNSGLLISKTEHMLEVARRARAVGYQANTHAIGDRGVRNVIDAYEQAGATPSDRFRIE
                     HLQVVAPSDFSRLAPHGIIASMQPTHATSDMGWAEDRLGSERIKGAYAWRTVLNSGAR
                     LALGSDFPVEFVNPFFGIYSAVTRQDQKGNPPGGWYPDQKLTLAEAIRGFTSDAAYAA
                     FEESSRGTIEPGKLADLTIVEGDLYAAPQSSLFATKVRYTVVGGEVVYGAK"
     gene            complement(2813..3730)
                     /locus_tag="VNN25_00605"
     CDS             complement(2813..3730)
                     /locus_tag="VNN25_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXH90049.1"
                     /translation="MGILVVGSVAFDAIQTPFGKVDRCLGGSATYFSVAASFFSEVDL
                     VAVVGDDFSDDDLAIFRGRRVNLDGLQRVAGAKTFFWSGEYGFDLNVAKTRDTQLNVF
                     ADFKPELSERHQSPDVLFLANIQPDLQLDVLRQCKRPRVVALDTMNLWIDVAKESLTR
                     ILREVDLVVINEAEVRQYTKEANLIKGARQILALGPRTLVIKRGEYGVMMITKDAVFA
                     APAYPLEAVFDPTGAGDTFAGGFLGYLASRSEASDHELRRAIVFGSVLASFTVEKFSL
                     DRLREITLDDVYERYQEFRKLTHFEDHEK"
     gene            complement(3831..4697)
                     /gene="mtnP"
                     /locus_tag="VNN25_00610"
     CDS             complement(3831..4697)
                     /gene="mtnP"
                     /locus_tag="VNN25_00610"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXH90050.1"
                     /translation="MTNATIGIIGGSGLYQMAGLSVLEEQSIETPFGDPSDAYVIGEI
                     DGVRVAFLSRHGRGHRLTPSELNYRANIFGFKLLGVHTILSASAVGSMKIEYAPTDIV
                     FPHQFIDRTRHRPDTFFGNGIVAHVGFADPICTAVSAAMSKVAREVGARVHEGGVYLC
                     MEGPQFSTRAESNLYRTWGVDVIGMTNLQEAKLAREAEICYATMALVTDYDCWHETHD
                     DVSVDAVLAYLAKNAETAQKILRSSIVPTATRERNCPCASALQYGIMTSPAAMPEKTK
                     ADLAPIIGKYLT"
     gene            complement(4694..5653)
                     /locus_tag="VNN25_00615"
     CDS             complement(4694..5653)
                     /locus_tag="VNN25_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXH90051.1"
                     /translation="MRVGVVGTGYLGRLHARILTEMPEAEVAGFVETDDVIAAEVSEN
                     LKLKRFASVDALAREIDCAVVATPTITHYDVSRQLIEAGCDVMVEKPITPAVADAQRL
                     IELAASRGRILQVGHVERYNPAIMAVAPLLHEVRFVEAQRLGVFVGRSLDIDVLLDLM
                     IHDLNLVLSLLGQRIVDVRAVGVAVLTDKVDITNVRLELENGAIANLTASRVSQDRVR
                     KIRFFSREAYISVDTKEQEVKGYGLSGKTIVPIDIVVNKQEPLRAELEAFLACVRTRT
                     RPLVSGEDGLAAVELAIRVADAIEESMRRFYPEDAARPGKEGK"
     gene            complement(5650..>6418)
                     /gene="lpxA"
                     /locus_tag="VNN25_00620"
     CDS             complement(5650..>6418)
                     /gene="lpxA"
                     /locus_tag="VNN25_00620"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXH90052.1"
                     /translation="DSTAVVSGNASLAPDVVVGAYAVIGANVTIGSGTVIGPFTRVEG
                     PTVIGERNQFYGHASIGGPPQDLKYKGEHTELAIGNDNVFREFVTINRGTTGGGGKTT
                     IGSDNFFMAYSHVAHDCHVGSNTVFANSATLAGHVEIADFATIGAFSAVHQFCRVGEH
                     AFIGGGSMITQDVLPYAKTVASRDNRSYGINTIGLERKGFSKETVEALQRAYRLLVRS
                     KLNVETALAKIEEELGLFPEARYLVEFVRESKRGIIR"
BASE COUNT         1188 a         2030 c         2041 g         1159 t
ORIGIN      
        1 cgggatcacg ttcggaagct cgctgcctcg tgtcaaacag tgctgaggcc ggtggcgttg
       61 cgtcctcccg tcgccgggag tttgcctttt cgcgtgatcg gtttttatgt gattgttcga
      121 aacgaaaccg cagcctttga tcttcctacg gatgagcact tttttcgcgc gagcgaagag
      181 gccggcacta ccgcttcaac gccatctcat atgctgcgcg aaccgttcga acgtagtcct
      241 ttgtctcgcg aaatggcggc acgccgttgt agcgctgcac agcgccggga ccggcgttgt
      301 aggcggccag cgtgaggtcg agatcgccgt ggaacgtatc gaggagggtg cggagatagt
      361 gaacgccgcc gaagacgttc tcgcgcgcat caaaggcatt tcgcacgccg gtgtaccggg
      421 ccgtcgccgg catcaattgc atcacaccga ccgcgccggc tggcgagact gcgccagggt
      481 tgtaggcgga ctctcgtgca gcaatcgccg cgacgaggcg gggatcgacg ccgtgttgct
      541 tcgaggcatc cgcgataagc gccgacaaat acgccggcat cggcgctgtc acccgcgctc
      601 cggaatcgaa caacgcggtg ccgaacggtg tgtaatgcat ggccctggga ccggagcgca
      661 gcgtgaagag cgcctggcgg atatcgccga agaagtgata cgccgtgacg ccgcgggcat
      721 cgttgcggat cacgacgcgg ccggtctgtc ggatgtgggc agatgggacg tccctcgcgc
      781 tgaaatcgcc cgcggccgcg gaggcggcca cgaacgctac gctgagtgca aataaagttc
      841 gcaagggctc tgagcaacgc gcgtcccgga agccgctatt ccgatacgat tcgcgcatga
      901 cgatcgtgcg acgcgccgct gcgctactct tcctcgcgtt taccgcctgt accatgcaat
      961 ctccctcaaa cgacaactcg gtcaaacggt cgaacgagac cattttcgaa ggcggcacgg
     1021 tgctggccgg tccgtcccaa actccgcagg cgaactggtc cgtcgtcact gcaaacggca
     1081 ccatcgtcgc tgccggaccc gccgacgcga ttcgggcagc gcatcccaac gcgcgtgtgg
     1141 tcgacgtcca cggcacgacc gtgatgcccg gtctcaccga cgctcacggg catctctatg
     1201 gactcggcct ttcgctcgat accgtcaacg tcatcggcac tacgtcgtac gacgaggtca
     1261 tcgcgaaggt tcgcgaccgc gcccaacgcg ccagcgccgg agattggatt ctcggacgcg
     1321 gttgggatca gaaccactgg cccggcaagc agttcccgac agccgcgccg ctcgatgccg
     1381 caatctccga ccatcccgtc tggattcgtc gcgtcgacgg ccacgccggc atcgccaaca
     1441 gtgccgccat gcgggcggca ggtgtcacgg cagcgacgcc cgatccggaa ggtggccgcg
     1501 ttctgcgcga tgccgcaggc aatccgaccg gtgtcttcat cgacggcgcg atgtcgctga
     1561 tcgactcgaa ggtcccggct ccgtcgccgg aacttcgtaa agcacgcgtg ctcgctgccg
     1621 cgcaggcgat cgccgagaac gggctcaccg aaatgcacga cgccggcgcg gaacaggcca
     1681 cgatcacggc gatccagcag ctcatcgacg agcacaagtt cccgatccgc gtttacctga
     1741 tgctgaccga cgagcccgcg ctgctcgaat catggttcgc gcgcaaacct ctcatcggat
     1801 acggcggccg cctcaccgtt cgctccgtga agatgtacgc cgatggcgcc ctcggcagcc
     1861 gcggcgccgc gttgctcgcg ccgtacagcg acgacccgtc gaacagcgga ctgctcatca
     1921 gcaaaaccga gcacatgctg gaggtggcgc gccgcgcccg cgctgtcggc tatcaggcca
     1981 acacccacgc gatcggcgac cgcggcgtcc gcaatgtgat cgatgcctac gagcaggcgg
     2041 gagccacgcc gtccgaccgt ttccgcatcg agcatctgca ggtcgtcgcg ccctctgatt
     2101 tttcccgcct cgcgccgcac ggcatcatcg cctcgatgca gccgacgcac gccacgtctg
     2161 acatgggctg ggccgaagac cgcctcggca gcgagcggat caagggcgcc tacgcctggc
     2221 gcaccgtgct caattccggc gcccgcctcg cccttggctc cgattttccg gtcgagttcg
     2281 tgaacccgtt cttcggcatc tacagcgccg tcacccgcca ggatcagaaa ggcaacccgc
     2341 ccggcggctg gtatcccgat cagaagctga cgctggccga agcgattcgc ggcttcacgt
     2401 ccgacgccgc atacgccgcg ttcgaagagt catcacgcgg cacgatcgag cccggcaaac
     2461 tcgccgacct cacgatcgtc gaaggcgatc tctacgccgc gccgcagtcg tctcttttcg
     2521 cgacgaaggt gcgctacacg gtcgtggggg gcgaggtggt gtacggagca aagtagctgc
     2581 acgcgttcga caccaaagcg cgatccgata tttgactgag atctcaattc atttcgcgcg
     2641 aagcgctttg gactgcggtg gcgcagccac cgctttgaat cacgcgttcg aagccgaaga
     2701 cccggaagca gcgggagaag ctcacgaaga ttgacctgcg cccaaaccct ctcttgaaag
     2761 cggtggctgg cgccaccgca gtccaaagcg cttcgcgcga aatgtatcca ccctactttt
     2821 cgtgatcctc aaagtgcgtc agcttgcgga actcctggta ccgctcgtac acgtcgtcga
     2881 gcgtaatctc gcgcaggcgg tcgagcgaaa acttctcgac ggtgaacgac gccagcacgc
     2941 tgccgaaaac gatggcgcgg cgtagctcgt ggtccgaggc ctcggagcgc gaggccaggt
     3001 agccgagaaa accgccggca aaggtgtcgc ccgcgccggt cggatcgaag acggcttcga
     3061 gcgggtacgc cggcgcggcg aagaccgcat ccttcgtgat catcatgacg ccgtattcgc
     3121 cgcgtttgat cacaagcgtg cgcgggccga gcgcgagaat ctggcgcgct cctttgatca
     3181 gatttgcctc tttcgtgtac tgcctcacct cggcctcatt gatcacgacg aggtcgacct
     3241 cgcgcaggat gcgggtcagc gactccttcg cgacgtcgat ccagaggttc atcgtgtcga
     3301 gcgcgacgac gcgcgggcgt ttgcactggc gcagcacgtc gagttgcaga tcgggctgga
     3361 tgttggccag gaacaggacg tcagggcttt gatgccgctc gctcaactcc ggcttgaaat
     3421 cagcgaagac gttcagttgc gtgtcgcggg tcttcgctac gttcaggtcg aagccgtact
     3481 ccccgctcca gaagaacgtc ttcgcgccgg ccacacgctg caagccgtcg agattgacgc
     3541 ggcgtccgcg aaagatggcc agatcatcgt cgctgaaatc gtcgccgacg acagcaacga
     3601 gatcgacttc cgaaaagaac gatgccgcga ccgagaagta ggtcgccgat ccaccgagac
     3661 agcggtccac tttcccgaac ggagtctgaa tcgcatcaaa cgcaacgctg ccaacaacga
     3721 ggatgcccat gaactctccc agcgcgagtt gcgcggcgcg gagagtagcg ggattgggcg
     3781 aagggagcaa caaggccgcg gcgcggcact cgcctgagcc gaaggcggat ctacgtcagg
     3841 tacttgccga taatcggcgc cagatccgct ttcgtcttct ccggcattgc tgcggggctc
     3901 gtcatgatcc cgtattgcaa cgcgctggcg catgggcagt tccgctcgcg ggttgcggtg
     3961 gggacgatgg agctgcgcag gatcttctgg gcggtctcgg cgttttttgc gaggtaggcc
     4021 agaaccgcgt cgaccgaaac atcgtcgtgc gtttcgtgcc agcagtcgta gtcggtgacg
     4081 agggccatgg tggcgtagca gatctcggct tcgcgagcga gtttcgcctc ctgaaggttc
     4141 gtcatgccga tcacgtcgac gccccaggtg cggtagagat tcgactcggc gcgggtcgag
     4201 aattgcgggc cttccatgca gaggtagacg ccgccttcgt ggacgcgcgc gcccacttcg
     4261 cgggcgacct tgctcatcgc cgcggagaca gcggtgcaga tcggatcggc gaaaccgacg
     4321 tgcgcgacga taccgttgcc gaagaaggtg tcggggcgat ggcgcgtgcg gtcgatgaac
     4381 tggtgcggaa aaacgatgtc ggtcggcgcg tattcgatct tcattgaccc gacagcgctg
     4441 gcagagagga tcgtgtgaac gccgaggagc ttaaagccga agatgttcgc gcggtagttc
     4501 aactcgctcg gcgtgaggcg atggccgcgg ccgtgacgcg agaggaaagc cacccgcacg
     4561 ccgtcgatct cgccgatgac gtaagcatct gacggatcgc cgaagggagt ctcgatgctc
     4621 tgctcttcga gtacgctcag gcctgccatt tgatagagac cggagccgcc gatgatgccg
     4681 atggttgcgt tcgtcatttg ccctctttac ccggccgcgc agcatcctcg ggatagaaac
     4741 gccgcatcga ctcttcgatt gcgtcggcca cgcggatggc cagctcgact gcggcaaggc
     4801 catcctcccc gctcacgagc gggcgcgtac gcgtgcggac gcatgcgagg aacgcttcga
     4861 gctccgcgcg taacggctcc tgcttgttga cgacgatgtc gatcgggacg atcgtttttc
     4921 cggacaggcc gtagcctttc acttcctgct ctttcgtatc gaccgaaatg taagcctcgc
     4981 ggctgaagaa gcggatcttg cgcacgcggt cctgggagac gcggctggcg gtcagattgg
     5041 cgatggcgcc gttctcgagc tcgagccgga cgttggtgat gtcgacctta tcggtgagca
     5101 ccgccacacc tacagcgcgt acatcgacaa tgcgctgccc aaggagtgaa agcaccaggt
     5161 tgaggtcgtg gatcatcagg tcgagcagga cgtcgatgtc gagcgaacgt ccgacgaaaa
     5221 cgccgaggcg ctgggcttcg acaaagcgga cttcgtgaag cagcggtgcg acggccatga
     5281 tggccgggtt gtagcgctcg acgtggccga cctgcaggat gcggccgcgc gatgcggcca
     5341 gttcgatgag acgctgcgcg tccgcgactg ccggtgtgat tggcttctcc accatcacgt
     5401 cgcagcccgc ttcgatcaac tggcgcgata cgtcgtagtg cgtgatggtc ggcgtggcca
     5461 caacagcgca atcgatctca cgtgccagcg cgtcgacgga ggcaaaccgt ttcagcttta
     5521 ggttttccga gacctccgcg gcaatgacat cgtccgtctc gacgaagccg gcgacctccg
     5581 cctccggcat ctcggtaaga atgcgcgcgt ggagccgtcc gaggtagccg gtgccgacga
     5641 caccaaccct catcggatga tgcccctctt cgattcgcga acgaactcga cgagatagcg
     5701 cgcctcggga aagaggccaa gctcttcctc gattttcgcg agcgcagttt caacgttcag
     5761 cttcgaacga acgagaaggc ggtaggcgcg ctgcaacgcc tccaccgtct ctttcgaaaa
     5821 gcctttgcgt tccagaccga tcgtgttgat tccgtagctg cggttgtcgc ggctggcgac
     5881 ggtcttagcg tacggcagga cgtcctgcgt gatcatcgag ccgccgccga tgaaggcgtg
     5941 ctcgccgact cggcagaact gatggaccgc gctgaatgcg ccgatcgtgg cgaaatcggc
     6001 gatctcgacg tgtcccgcca gcgtggcgct gttcgcaaag accgtgttcg aaccgacatg
     6061 acagtcgtgt gcaacgtgcg aataagccat gaagaagttg tccgagccga tcgtcgtctt
     6121 gccgccgccg ccggtcgtgc cgcgattgat ggtgacgaac tcgcggaaga cattgtcgtt
     6181 accgatggcg agctcggtgt gctcgccttt gtacttgaga tcctgcggcg gacctccgat
     6241 cgaagcgtgg ccataaaact gattgcgctc gccgatcacg gtcggacctt cgacccgcgt
     6301 gaaggggccg atgacagttc cgctgccgat ggtgacgttc gcgccgatga ccgcgtaggc
     6361 gccgacgacg acgtccggcg caagggaagc gttgccgctg acgactgctg tcgaatcg
//
LOCUS       DATLVW010000019         4658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_811, whole genome shotgun sequence.
ACCESSION   DATLVW010000019 DATLVW010000000
VERSION     DATLVW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VNN25_00625"
     CDS             <1..706
                     /locus_tag="VNN25_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXH90053.1"
                     /translation="LVDNYDLSSLTTLFSGAAPLSPEVAAAATKRLHCSFTQGYGMTE
                     ASPCTHATRVGQTEGVGEPMPNTESKIVNLESGAELGPNEEGEICVRGPQVMKGYLNR
                     PDATAAMIDAAGWLHTGDVGYADDRGRFFIVDRVKELIKYKGMQIAPAELEALLLAHP
                     AIADAAVIPIADEEAGQVPKAFVVLRSGVTTEEIQSFVALRVAPYKKLRHVEITDTIP
                     RSPSGKILRRVLAARG"
     gene            834..1115
                     /locus_tag="VNN25_00630"
     CDS             834..1115
                     /locus_tag="VNN25_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90054.1"
                     /translation="MTTTTTVERVQTGIRIEKRILKVLKGMAEVHDMSLGDLIEGIVL
                     HAFEGKAPFGSESLKKIGELRKIYGLTLSAADSHKLIEDSSPRKRKKAS"
     gene            1112..1528
                     /locus_tag="VNN25_00635"
     CDS             1112..1528
                     /locus_tag="VNN25_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90055.1"
                     /translation="MTDSDFVDAFEACTLPNGSFHHRDHVRLAWIYLRDLPLLAALDR
                     FTTSLQRFAAANGRPELYHATITWAYLLLIHERMERMPADDFESFARGNADLLTWKPS
                     VLDVYYDRATLDSELARRTFVLPIKSDPQMTQMNAD"
     gene            1649..2038
                     /locus_tag="VNN25_00640"
     CDS             1649..2038
                     /locus_tag="VNN25_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXH90056.1"
                     /translation="MTDRQPPRPTDSELAILGVLWERGPSTVRDVHEALNKDGSTGYT
                     TVLKILQIMTEKGLVVRDETERAHVYESRYGEQKTQRQLLADLAERAFGGSATKLVMQ
                     ALSGRKASADELTAIRNLLDTLEGEKR"
     gene            2035..4206
                     /locus_tag="VNN25_00645"
     CDS             2035..4206
                     /locus_tag="VNN25_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXH90057.1"
                     /translation="MNGLGNLLGTPLAQAIGWALVHLLWQGLLVAAILAATLALLSKQ
                     SANARYLASCGALVVLVILGAATAYRSYEGGRLSGVGSRSETGTIALPASLETSSSSE
                     GALSSDTRLPTPDTRFELATFAKAQLPKVVLIWLVGVLLLSVRLLFGWLRAHAIAKRN
                     ATDAAPEWQRAARRLAHALDLRRAVQLLESAAVEVPTVIGWLRPVVLLPAATLTGLST
                     EQMEMILAHELAHVRRNDFFVNLMQAVVETLLFYHPAVWWISNRIRVEREHCCDDVAV
                     SVTGNALVYARALTRLEELRVADAQAFIAANGGSLIGRIRRLAGARVESPNAPSRFVA
                     GAALLVVLVALIVAPSLPLRGEQQKAVDAKATPKPQTAQSSVEVRAETAESDSEAETP
                     EADVNDAMVPVIAPRAAIEGGVRGGVEGGVEGGVEGGIAGGIAGGVTGGIADGVTVNV
                     APMAPRAFRWEGQPVIAVAPVARATVTPRVAVTVAPTVRAIAATAPRIAMSAIRGYEY
                     AWEQETPEPPEPPDTPGTPRAARTPRPPRLPRARHHIGEGGKLTVDDLIELRACGVTP
                     AYIDSMRGAGMGQLSLDEIAEMKSVGVTPEYLSSLRAAGIKFDSARNVTELRAMGITA
                     EYAAQMRAAGYGNLSTKQLVEMRAMGVTPAYVKQINDAGYHNLTPKDLVELRAQGVNP
                     EFIKSLNDAGYTNLSVKDLVRMAAMGVNADFIRDLAKYKVK"
     gene            4339..>4658
                     /locus_tag="VNN25_00650"
     CDS             4339..>4658
                     /locus_tag="VNN25_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90058.1"
                     /translation="MRRSLALALFLFLAAFTARAAQHGAWHLTNHDDSRDDGKVQLQL
                     VSDHNQNSHSMDRSEFTGLTAAQMSATAETPVDFNLIRDAGTIHFTGTFLNGEGVGRF
                     TFAP"
BASE COUNT          894 a         1519 c         1421 g          824 t
ORIGIN      
        1 gctcgtcgac aactacgacc tcagctcatt gaccacactc ttctccggcg ccgcaccgct
       61 cagccccgag gttgctgcgg cggcgacgaa acggctgcac tgctcgttca cgcagggcta
      121 cgggatgacc gaggcgtcac cgtgtacgca tgcgacacgg gtcggccaga ccgaaggcgt
      181 gggcgagccg atgccgaata cggaatcgaa gatcgtcaac ctggaaagcg gcgcggagct
      241 ggggccgaac gaagaaggcg agatctgcgt tcgcggtccg caagtgatga agggttatct
      301 gaaccgccct gacgccaccg ccgcgatgat cgacgccgcg ggctggctgc acactgggga
      361 cgtcggatac gccgatgacc gcggccgctt ctttatcgtc gaccgcgtta aagagttgat
      421 caaatacaaa gggatgcaga tcgctcccgc cgagctggaa gcgctgctcc tcgcccaccc
      481 cgccatcgcc gacgcggcgg tgattccgat tgctgacgaa gaagccggtc aagtgccgaa
      541 ggccttcgtc gtgctgcgct ccggcgtcac cacggaagag atccaatcgt tcgtcgccct
      601 gcgcgtcgcg ccgtacaaga aactgcggca tgtggagatc acggacacga ttccgcggtc
      661 gccttcgggg aagatattgc ggcgggtgtt ggcagcgcgc gggtgattcc gaagtcgcgt
      721 tcacatgaac caccacaaag acacaaagga cacagaggtt ttctctgtgc tctctgtgtc
      781 tctgtggtga ttctttctcg tctgctatct gtgtaccgtg gtactattgt cccatgacaa
      841 caaccaccac cgtagaacgc gtacagaccg ggatacgcat cgagaagcgg attctcaagg
      901 tgctcaaagg gatggccgag gtgcatgaca tgtcgctagg ggacctgatc gagggaatcg
      961 tgctgcatgc cttcgagggg aaggcgccgt ttgggagcga gtcgctgaag aagatcgggg
     1021 agcttcggaa gatctacggg ctgacgttga gcgcagccga ctcgcacaag ttgattgagg
     1081 attcgtctcc gcggaagcgg aagaaggctt catgaccgac tccgacttcg tcgacgcatt
     1141 cgaggcgtgc acgctgccta acggcagctt tcatcaccgc gatcacgttc gtctggcctg
     1201 gatctatctg cgcgatcttc ccctccttgc cgcactcgat cgcttcacga catcgctcca
     1261 gcgctttgcc gctgcgaacg gacgtcctga gctctaccac gcaacaatca cctgggctta
     1321 cctgctgttg atccacgagc ggatggagcg catgccggcg gacgacttcg agtcgttcgc
     1381 tcgaggcaac gccgacctct tgacctggaa gccgtccgtg ctcgacgtct actacgaccg
     1441 cgcgacgctc gattcggagc tggcccggcg gacgttcgtc ctgcccatca aatctgatcc
     1501 gcagatgacg cagatgaacg cagattaaga gccgctttta tctgtgcctc atctgcgcca
     1561 tctgcggatc aaaccgctca ctacgaaact cttcgttgac atccacgaaa aacttcgtat
     1621 gattgcttgc gtacaagagg aaaccaccat gaccgaccga caaccgccac gaccaaccga
     1681 ctccgagctt gccatcctgg gcgtgctttg ggagcgcgga ccgagcaccg tccgcgacgt
     1741 ccacgaagca ctgaacaagg atggatccac cggctacacc accgtcctga agattctgca
     1801 gatcatgacg gagaaaggtc tggtcgttcg cgacgagacc gagcgcgccc atgtctacga
     1861 atcgcgctac ggcgaacaga agacgcagcg ccaactcctc gccgatctcg ccgagcgggc
     1921 cttcggcggc tccgccacca aactcgtgat gcaggcactc tcggggcgca aagcaagcgc
     1981 cgacgagctg actgccattc gcaacttgct cgacaccctg gaaggagaaa aacgatgaac
     2041 ggactgggca acctactggg cactcctctg gcgcaggcga tcggctgggc gctggttcac
     2101 cttctgtggc agggtctgct cgtcgcggcc atcctcgccg ccacgctggc actcctttcg
     2161 aagcaaagcg cgaatgcgcg atacctggcc tcctgcggcg ccctcgtcgt gctcgtgatt
     2221 ctcggcgcgg cgacggcgta tcgttcttat gaggggggtc gcctttcggg agtcgggagt
     2281 cggagcgaaa ccggcaccat cgcactcccg gcgagcctcg agacttcctc atcaagcgag
     2341 ggagctctca gctccgacac ccgactcccg actcccgaca cccgcttcga actggcaacc
     2401 ttcgccaaag cccagctccc gaaagtcgtc ctcatctggc tcgtgggcgt cctgctgctg
     2461 tccgtgaggc tccttttcgg atggctgcgg gcgcatgcga tcgcgaaacg caatgccacg
     2521 gatgctgcgc cggaatggca gcgcgcggcg cggcggctgg cgcatgcgct cgaccttcgc
     2581 cgggccgtgc agcttctcga gtcggcggct gtcgaagtac cgacagtcat cggatggctt
     2641 cgtccggtcg tccttcttcc cgcggccacg ctcaccgggc tctccaccga acagatggag
     2701 atgatcctcg cgcacgagct ggcgcacgtc cgccgcaacg acttcttcgt caacctgatg
     2761 caggccgtcg tcgaaacgct gctcttctac catccggcag tgtggtggat ctctaaccgc
     2821 atccgcgtgg agcgcgagca ttgctgcgat gacgtggccg tctccgtcac cggcaacgcc
     2881 ctcgtttacg cccgtgcgct gacccgtctc gaagagctgc gcgtcgccga tgcgcaagcc
     2941 ttcatcgccg cgaacggcgg atcgctcatc ggccgcattc gccgtctggc gggagcgcgc
     3001 gtcgaatcac cgaacgctcc gtcgcgcttc gttgctggcg ccgcgctgct cgtcgtcctc
     3061 gtggcgctga tcgtcgcgcc gtcgcttccg ctgcgcgggg aacagcaaaa ggccgtcgat
     3121 gcgaaagcga caccgaagcc gcagacggcg cagagcagtg tcgaagtgag agcagaaacg
     3181 gccgaatcgg atagtgaggc tgaaacaccc gaggcggatg tgaatgatgc gatggttccg
     3241 gttattgcac cgcgcgcagc catcgaaggc ggcgttcgcg gcggcgtgga aggtggtgtc
     3301 gaaggcggag tcgaaggcgg gatcgccggt ggcatcgccg gaggagttac cggaggaatc
     3361 gcggacggcg tcaccgtgaa cgtcgcaccg atggctccac gcgccttccg gtgggaaggc
     3421 cagccggtca tcgccgtcgc gccggtggcg cgcgcgaccg tgacgccgcg agtcgccgtc
     3481 accgtcgccc caaccgttcg tgcgatcgca gcgaccgcgc cgcgaatcgc catgagcgca
     3541 atccgcggct atgagtacgc ctgggagcag gaaactcccg agccacctga gccgcccgac
     3601 acgcctggta ctccgcgtgc ggcacgcacg ccgcgtcctc cgcgcctgcc gcgtgcgcgt
     3661 catcacatcg gcgaaggcgg caagctgacg gtcgatgatc tgatcgagct ccgcgcttgc
     3721 ggcgttaccc ccgcgtacat cgactcgatg cgcggcgcgg gaatgggcca gctttccctc
     3781 gacgagatcg ccgagatgaa atccgtcggc gtgaccccgg agtacctcag cagccttcgc
     3841 gctgccggca tcaaattcga tagcgcccgc aacgtcaccg agttgcgcgc catgggtatc
     3901 accgccgaat acgccgcgca gatgcgcgcc gccggatacg gcaacctcag caccaagcag
     3961 ctcgtggaga tgcgggccat gggtgtcaca cccgcgtacg tcaagcagat caatgacgcc
     4021 ggctaccaca acctgacgcc gaaggatctc gtcgagctgc gggcgcaggg agtcaatccc
     4081 gagttcatca agtcgctcaa cgatgccggc tacacgaacc tgtcggtgaa ggatctcgtc
     4141 cggatggccg cgatgggagt gaacgcagac ttcattcggg atctggccaa gtacaaggtg
     4201 aagtgagtta acgccagcgg tcaggtagcg atgcctggcc gctggcgacg aaccattgct
     4261 tctaactttc gcgcaatcgc tacgtccaat tgaccactgg atgttacgaa cgccccgaca
     4321 acgataagga gacccaccat gcgccgttcc ctcgcactcg ccctcttcct ttttcttgcc
     4381 gcgttcacgg cgcgcgccgc gcagcatggc gcgtggcatc tgacgaatca cgacgattcg
     4441 cgcgatgacg ggaaggtgca actacagctc gtctccgatc acaaccagaa cagccactcg
     4501 atggatcgca gcgagttcac cggactcacc gccgcgcaga tgagcgccac ggccgagacg
     4561 ccggtcgatt tcaatctcat ccgcgatgcg ggcacgatcc atttcaccgg cacgttcctc
     4621 aacggcgaag gcgtcggcag gttcacgttc gctcccaa
//
LOCUS       DATLVW010000020         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_497240, whole genome shotgun sequence.
ACCESSION   DATLVW010000020 DATLVW010000000
VERSION     DATLVW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497240"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..2130
                     /locus_tag="VNN25_00655"
     CDS             316..2130
                     /locus_tag="VNN25_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HXH90059.1"
                     /translation="MKRLGLPVLALVLLSCQSLLAWNGTGHELVAGIAWDNMSPDARN
                     KAIALLESAPSNACLLDLFPNDSRPLAVRQREFFMRAATWPDVVRPKGQHDLRACTRF
                     HQREWHFVDRFWKGVSGSTGGDAPTDDTAIPIVSTNAVERLTTFRGTVNCETCGTASE
                     RATQLAWIMHLTGDIHQPLHAESRVTDANPAGDRGGNLFLLAPKSISPLHTFWDRIID
                     NSILEKPEEKPPNQQIVYLDRVIGMIVHDHPKASEAARLKPGDFDAWSLEGFETAKSS
                     AYPASLHENQTPAPAYKKNVFAIADEAIAMAGYRLADLLETTLGAGGGTIVTTRLPAP
                     SHIVIVIEENKSFGNVIGSPKAPFINGLASRGALLTKFFASHHPSQPNYIDFFAGDTL
                     GVCKDTCPIGPFTSQNLGAALIGAGKSFAGFSENLPPSGARTTCNNSVFAPKHCPWVD
                     FTNVPDSASKDFTQFPHDAAGFAALPDVSFVIPNLNDDMHNGSRITSEVGAGDTWLQT
                     NLSAYADWAEQNNSLLIVTWDEDSSSYTTHCPGTVITTTPPKNHIPTIVMGQPVTPGT
                     QSSTSYTHHDLLRTLLDIYGIAPFGGATTGKDITDIWR"
BASE COUNT          456 a          743 c          676 g          412 t
ORIGIN      
        1 cacgacgtga cgatcagcgc agcgccgccg agcagtccaa gagccagagt gaaagccacc
       61 ggtcgatgcg ttgcagttcc cgtcattgtt gcctccgcgc ctgcagtcta tctcgtttca
      121 ggcgcggctg ccggtatcgg aagcaatccg cagatgacgc agatgagagt tgtctgcatc
      181 tgtgtccatc tgcgtcatct gcggatcggt ccctcgcatc acggcagggt agggaactgt
      241 gtccaaccgg actctcctca agtaggttgc cggccctgat gaaccgggct tccccggcgg
      301 ccagaaggat gttctatgaa acgcctcggt cttccggttc ttgctctcgt tctcctgtca
      361 tgccagtcgc tccttgcctg gaacggtacc ggtcatgagc tggtcgccgg gatcgcctgg
      421 gacaacatgt cgccggacgc ccggaacaag gcaatcgcgc ttctggagag cgctcccagc
      481 aacgcctgcc ttctcgatct cttcccgaat gattcgaggc cgctcgcagt ccgccagcgc
      541 gagttcttca tgcgtgccgc gacctggccc gatgtcgtcc ggccgaaggg ccagcacgat
      601 ctacgcgcgt gcacgcgttt ccatcagcgg gaatggcatt tcgtcgacag gttctggaag
      661 ggagtctcgg gcagcaccgg cggcgacgcg cccaccgacg acacggccat ccccatcgtg
      721 tcaaccaatg ccgtggaacg gttgacgacg tttcgcggca cggttaactg cgagacatgc
      781 ggaacggcct cggaacgcgc gacgcagctc gcctggatca tgcacctcac gggcgacatc
      841 catcagccgc tccacgcgga gtcccgcgtc acggacgcga atccagccgg cgaccggggc
      901 gggaacctct ttctcctcgc accgaagtcg atctctcctc tgcacacctt ctgggatcgg
      961 atcatcgaca actcgattct ggagaagcca gaggagaagc cgccgaatca gcaaatcgtc
     1021 tacctcgacc gcgtcatcgg catgatcgtg cacgatcatc cgaaggcgtc cgaggccgca
     1081 aggctgaagc cgggcgactt cgatgcgtgg tcgctggagg ggttcgagac ggcgaagagc
     1141 tcggcgtatc cggcaagcct gcatgagaat cagacgccgg ctcccgccta caagaagaac
     1201 gtgttcgcga tcgcggacga agcgatcgca atggctggct atcggctagc cgatctgctc
     1261 gaaaccacgc tgggcgccgg cggcggcacg atcgttacca cgaggcttcc ggctcccagt
     1321 cacatcgtga tcgtgatcga ggagaacaag tcgttcggca atgtcatcgg ctcgccgaag
     1381 gcgccgttca tcaatggtct ggcgtcgcgt ggcgcgctgc tcacgaagtt ctttgcctcg
     1441 catcatccta gccagccgaa ctacatcgat ttcttcgccg gcgacacgct cggcgtctgc
     1501 aaggacacct gcccgatcgg cccgttcacc tcccaaaacc tcggcgcagc gctgatcggc
     1561 gcgggcaagt ccttcgccgg cttctcggag aatctgccgc cgtccggcgc acgcacgacg
     1621 tgcaacaact ccgtgttcgc tccgaagcat tgtccgtggg tcgatttcac gaacgtgccg
     1681 gactccgcga gcaaagactt cacccagttc cctcacgatg ccgccggttt cgcggcgctg
     1741 cccgacgtca gcttcgtcat cccaaacctg aatgacgaca tgcacaacgg cagccgcatc
     1801 acctcggagg tcggcgcagg ggacacgtgg ctgcagacga atctcagcgc gtacgccgac
     1861 tgggcagagc agaacaacag cctgctgatc gtgacgtggg acgaagacag ctcctcgtac
     1921 acgacccatt gtccgggcac cgtgatcacg acgacaccgc cgaagaacca catcccgacg
     1981 attgtgatgg gtcagccggt gacgcccggc acgcagtcgt cgacgagcta tacgcatcac
     2041 gacctcctgc gtacacttct cgacatctac ggcatcgcac ctttcggagg ggcaacgacg
     2101 gggaaggaca tcacggatat ctggaggtag gggctcgggt gaaaggtcat tcgctgcttc
     2161 gctggcttgc gctcgcatgt gggaccagga acaccccgag cagcgtcata accgaattga
     2221 caggcacggc aaactgcaac aacaaggtct tttttgaacc acagagacac agaggacaca
     2281 gagattg
//
LOCUS       DATLVW010000021         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_579812, whole genome shotgun sequence.
ACCESSION   DATLVW010000021 DATLVW010000000
VERSION     DATLVW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579812"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VNN25_00660"
     CDS             <1..530
                     /locus_tag="VNN25_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXH90060.1"
                     /translation="PDPRQTTPSDDKLARFREEAGRKREGFIEEEAASSAGDQVELLT
                     FVLAGEQYAVSIDHIVEIVTPRPTTPIPNADRSIVGIMSLRGTIVTVVDVRQKLGHPP
                     RPGSSDTRTIVAERNGETLGFEVDRVLRVLKVGTTAIEPHPVVHASEQSEAIRGVFRH
                     ANALTILLDLDKLLA"
     gene            540..1397
                     /locus_tag="VNN25_00665"
     CDS             540..1397
                     /locus_tag="VNN25_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH90061.1"
                     /translation="MELTCKQCGARFRAAIPEPLTRAVRLACPSCSNQMVLKPASETA
                     ATASSDNPPPQRPSAHTARRRIAVIADEPRPFRAFLAEHLRRLGFDATTFESGEQAFE
                     FIRRSRADLAIVNVYLKGRLGVEVTEDIRADTALEHTRVILIGALFRANRFRANPTNL
                     YGADEYIEEQIPEREFRQIIRKLFPQVGHTEELPVEPKEYDEARRLARLILSDIVIYH
                     PGKVEAGIRDDNFFEVLKEEIAEGRNYYDSRVPLRVRQDTEIFSETLQLFVRMKREEA
                     GRLMTGTER"
     gene            1394..3610
                     /locus_tag="VNN25_00670"
     CDS             1394..3610
                     /locus_tag="VNN25_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH90062.1"
                     /translation="MNADPQLLEQARSSDADARQKAAYLVAHMVDERNYPLMFELLGD
                     KDWRVRKTIVDGLLHDPQPEVVTGLLDALADPENAGKRNSATEALVRIGEPAIRPIVY
                     RLRIEKDVDVRLSLVNLLGDLRSSDAFDILVESLEGEPDINVSSSIVSSLGKYRDAAA
                     LPHLMRVLKRDDLWLKFHAVEAFGEIGDRAALPAIMPLYAEKSLRKPVLEAIGKIADV
                     GTVSFLLRIIASEERLNLTALRALVRIAEASKPKIVEQAERTLIQRKFRESFPESKIG
                     PLIDHLHATPKRDVRSFILKFLGWSGDMRALPVLMNCLQHPDSAETAAQALTDFGDGA
                     VPAVLEMLRTAEEDEIIALLLRVLNVIGGRDAGPNILPLLDHENALIRRLAIETLGEI
                     TDPRSIDYLLAKLDDTDVASQQAAVNAISGLVAAYPEKKVEVLTRIRRLLQAPSVPMK
                     LNSLSVFVNIQGDGYHDELLLASKDSDPVIRQKAISLMGKFSEERFGDQLVLSLTDEA
                     TAVRLAAINAIVRHLPAQGLDPLISALEDSDIWIRTSAAQALGEYRHPDALQPLMHHL
                     AHDLPPVRIAAIEALGKSGDERVKDVLFNCAHESDLEVRRAAILALGRVPGNEVFDYL
                     MFATTDDDWRVRAAAVTAVAVRGDLTALPALHVALQDPDTYVQQSAVMALDKLPDRSS
                     FPHLFRALENSAILDEVSDLFVRHKQLYRDLLEEAWRTADSRREVVIAAILQAMKG"
BASE COUNT          738 a         1250 c         1130 g          690 t
ORIGIN      
        1 cccccgaccc ccgacaaacg accccctccg acgacaaact cgcccgattc cgcgaggaag
       61 ccggcaggaa acgcgaaggc ttcatcgaag aagaggcagc gagcagcgcc ggcgatcagg
      121 tggaactgct cacattcgtc ctcgccggcg agcagtacgc cgtatcgatc gatcacatcg
      181 tcgagatcgt cacgccgcgc ccgacgacgc ccattccgaa tgccgatcgg tcgatcgtcg
      241 ggatcatgtc actccgaggg acgatcgtga ccgtcgtcga tgtgcggcag aagctcggac
      301 atcctcctcg gcccggaagt agcgacacac gaacgattgt tgccgagcgc aacggtgaga
      361 ctctcggatt cgaagtcgac cgcgttttgc gcgtgctcaa agtcgggaca acggccatcg
      421 aaccacaccc ggtcgtccat gccagcgagc aaagcgaagc cattcgcggc gtcttccggc
      481 acgcgaatgc gttgacgatc ctcctcgatt tggacaagct actggcctga cttgagatca
      541 tggaacttac ctgcaagcaa tgtggcgcga gattccgcgc ggcgatcccc gagccgttga
      601 cgcgcgcggt gcgtctggct tgtccttcgt gcagcaacca gatggtgctc aagccggcgt
      661 ccgagacggc ggctacggct tcgtcagaca acccgccgcc gcaacgcccc tcggcacaca
      721 cagcgcggcg gcgcattgcc gtcatcgccg acgagccgcg tcccttccga gcctttctgg
      781 ccgagcatct gcgccggctc ggcttcgatg cgacgacgtt tgagagcggc gagcaagcct
      841 ttgaattcat tcgccgctcc cgcgccgacc tcgccatcgt caacgtctac ctcaaaggca
      901 gactaggcgt cgaagtgacc gaagacatcc gcgccgatac cgcgctcgag cacacccgag
      961 tcatcctgat cggcgccctc ttccgcgcca accgcttccg cgccaatccg acgaatcttt
     1021 acggagccga cgaatacatc gaagagcaga tccctgagcg cgagttccgc cagatcatcc
     1081 gcaaactctt cccgcaggtc ggccacaccg aggagctgcc ggtcgagccg aaggaatacg
     1141 acgaagcgcg gcgcctggcc cgactcatcc tcagcgacat cgtgatctac cacccgggaa
     1201 aagtggaggc cggaatccgc gacgacaact tcttcgaggt gttgaaggag gagatcgccg
     1261 aaggccgcaa ctactacgac tcgcgcgtcc ccctgcgcgt tcgccaggac acggagattt
     1321 tctccgagac gctgcagttg ttcgtacgca tgaaacggga agaagccggg cgactgatga
     1381 cagggacgga acgatgaacg ccgatcccca actcctcgaa caggcccgct cgtccgatgc
     1441 cgacgcgcgg cagaaagcgg cctatctcgt cgcgcacatg gtcgacgagc ggaactatcc
     1501 gctgatgttc gagctcctcg gcgacaagga ttggcgcgtt cgcaagacca tcgtcgacgg
     1561 cctcctgcac gatccgcagc ccgaagtcgt caccgggctg ctcgatgcgt tggcggatcc
     1621 ggagaacgcc ggcaagcgca actccgcaac cgaagcgctg gtcagaatcg gcgaaccggc
     1681 gatcaggcca atcgtctacc gtcttcgcat cgagaaggac gtcgacgtcc gcttgtcgct
     1741 cgtcaatctc ctcggcgatc ttcgcagcag cgacgctttc gacatcctcg tcgagtcact
     1801 ggaaggagag cccgacatca acgtctcctc ctcgatcgtt tcctccctcg gcaagtatcg
     1861 cgatgccgcg gcgttgcctc acctcatgcg cgtcctgaag cgcgacgatc tgtggctcaa
     1921 gtttcacgcc gtggaggcgt ttggcgagat cggcgatcgg gctgcgctgc ccgcgatcat
     1981 gccgctgtac gcggagaagt cgcttcgcaa accggtgctc gaagcgatcg gcaagattgc
     2041 cgatgtcgga accgtatcgt tcttgctgcg gattattgcg tccgaggaga ggctgaacct
     2101 gacggcgttg cgggcgctcg tgcggattgc cgaggcgagc aaaccgaaga tcgtggaaca
     2161 ggcggaacga acgctgattc agcggaagtt ccgcgagtcg ttcccggaat cgaagatcgg
     2221 tccgctcatc gatcacctcc acgccacgcc caagcgcgat gtccgctcgt tcatcctcaa
     2281 gttcctcggg tggtccggcg acatgcgggc gcttccggtg ttgatgaact gcctgcagca
     2341 tcccgactcc gccgagacgg ctgcgcaggc tctgaccgat ttcggcgacg gcgctgtgcc
     2401 agccgttctg gaaatgcttc gcacagccga agaggacgag atcatcgcgc tgctgctgcg
     2461 cgtgctgaac gtcatcggcg ggcgcgacgc cggtccgaac atcctccctc tcctcgatca
     2521 cgaaaacgct ctcattcgcc gattggcgat cgagacgttg ggtgagatca ccgacccgcg
     2581 ctcgatcgac tacctgctcg cgaagctcga cgacaccgat gtggcttcgc aacaggccgc
     2641 ggtcaacgcc atctccggtc tcgtcgccgc ctatccggag aaaaaagtcg aggtgctgac
     2701 caggatacgc aggctgctgc aagcgccctc ggttccgatg aaactcaact ctctctccgt
     2761 gttcgtcaac atccagggag acgggtatca cgacgaactg ctcctggcct cgaaggacag
     2821 cgatcccgtg atccggcaga aggccatctc gctgatgggg aaattcagcg aagagcggtt
     2881 cggcgatcag ctcgtgctct cgctcaccga cgaagcgacg gcggtgcggc tcgcggcgat
     2941 caatgcgatc gtccggcatc ttccggcgca ggggctcgat ccgttgatca gcgccttgga
     3001 agactccgac atctggattc gcacctccgc cgcgcaggcg ctcggcgaat accggcatcc
     3061 cgatgccttg cagccgctca tgcaccatct cgcgcacgac ctaccgcctg ttcgcatcgc
     3121 cgcgatcgag gccctcggca agtcaggcga cgagcgggtg aaggacgtgc tcttcaactg
     3181 cgctcacgaa tccgatctcg aagttcgccg cgccgccatc ctcgcgctcg ggcgcgtgcc
     3241 cggcaacgag gtcttcgact acctgatgtt cgcgacgacc gacgacgact ggcgtgtgcg
     3301 tgccgcggcc gtaaccgcag ttgccgtgcg cggcgatctc accgcgctgc cggcgctgca
     3361 tgtggccttg caggatccgg atacctacgt gcagcagtcg gccgtcatgg cgctcgacaa
     3421 actgccggac cgttcgtcgt ttccgcatct gttccgggcc ctcgagaaca gcgccattct
     3481 cgacgaggtc tcggatctct ttgtccggca caaacagctc taccgcgatc ttctggagga
     3541 ggcgtggcgc acggccgaca gccggcgcga ggtggtgatc gcggcgattt tgcaggcaat
     3601 gaaggggtag tggattcgta gattagtcga tcagtggatc agggttcctt tttgtcctga
     3661 tcgactgatc cactaatcca ctaatcaact ggcaatgtcc acattcaatc tgtccctgaa
     3721 tcaccatctc gatctgccgg acgacgtgtt caggctgatg cgggatcaga tttataagcg
     3781 gacgggaatg tggttctccg acaactcg
//
LOCUS       DATLVW010000022         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_125304, whole genome shotgun sequence.
ACCESSION   DATLVW010000022 DATLVW010000000
VERSION     DATLVW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125304"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VNN25_00675"
     CDS             complement(<1..1210)
                     /locus_tag="VNN25_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXH90063.1"
                     /translation="MLIATLLFATLLFASTASAQTSRPGDGIGAAQVDAIYPDIEKLY
                     IDLHQNPELSLHEQRTAATLAARVKALGYEVTTGVGGTGVVAILRNGPGPVVMLRTEL
                     DALPIEEKTGLPFASTVKTKNDAGDVVSVSHMCGHDVHMAAWSGTAELMAKNRAHWHG
                     TLMLVGQPAEEVVAGATAMLRDGLFTRFPKPDYALGIHDEPSLPAGVIGFHSGYFRAN
                     STGLDMTVYGKGGHGAYPQTAIDPVVIAARIVLGLQTIVSRENDPNDPAVVTVGSIHG
                     GSASNIIPDQVKLQITVRSLDPAVQKRLIAAIERQAKFEALAANAPKEPLVETKSNTD
                     AVYNDPALTQRMVAAARTAMGAGQVVEMPAQMGGEDFSQFGLAGVHAVLLHVGAVDAA
                     KLEASHKSGVP"
     gene            complement(1451..1837)
                     /locus_tag="VNN25_00680"
     CDS             complement(1451..1837)
                     /locus_tag="VNN25_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH90064.1"
                     /translation="MRRVTGIGGIFMSAQDPKALCDWYKKHLGIDVQPWGGAAFDWTD
                     AEGNPTKGTTAWSIGPADGKHFAPSKAAFMVNYRVEDLTALLKALREEGCNVLEKTDD
                     GEYGKFGWVMDPEGNKVELWQPPQGQ"
     gene            complement(1930..2478)
                     /locus_tag="VNN25_00685"
     CDS             complement(1930..2478)
                     /locus_tag="VNN25_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXH90065.1"
                     /translation="MRPLRYSINVTLDGCCDHREAFADEDLHRHAVENLAQADALLFG
                     RVTYEMMEAAWRPPAGTGARPDWMEPFARTIDAAKKYVVSSTLERVDWNAELVRGDLA
                     TAVQRLKTEPGKGLFTGGVKLPMALAELGLIDEYELVVHPRIAGHGPALFAGLSKRID
                     LKLVSRLELRSGAVAMRYEPRR"
     gene            complement(2616..3269)
                     /locus_tag="VNN25_00690"
     CDS             complement(2616..3269)
                     /locus_tag="VNN25_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90066.1"
                     /translation="MRDFLASKYMNILTQYGVGHGAFAGETSVAAVAAEISDKDIHTI
                     IQKAIDDGTIMEPGPNDCLVIFLDDGIGVKDADTTMCEPTSDDAFGYHFFFKTSKGHD
                     FFYAVIPSLNDACIHNTCPDPTQCSLQVGQTREQRQTQVTTHEVSEMLTDPKLTAWFD
                     DDGGSENGDICNGEPGQITVGANTWTVQKMYSKVDDVKTKGQTICVVAPKKPLPAAH"
BASE COUNT          629 a         1153 c         1113 g          741 t
ORIGIN      
        1 gcggcacgcc ggacttgtgc gaggcttcca gcttcgcggc atcgaccgcg ccgacgtgca
       61 gcaacacggc gtgaacgcct gccagtccga attgcgagaa gtcttctccc cccatctgtg
      121 ccggcatctc gaccacctgg ccggcgccca tcgcggtgcg ggcggcggcg accatgcgct
      181 gcgtcagcgc gggatcgttg tagacggcgt cggtgttgga cttggtctcg accagcggtt
      241 ccttcggcgc attcgcggcg agcgcttcga atttcgcctg gcgctcgatt gcggcgatca
      301 ggcgcttctg gaccgcgggg tcgagcgagc gcacggtgat ctgcagcttc acctgatcgg
      361 ggatgatgtt ggaggcgctg ccgccatgaa tgctgccgac cgtgaccacg gcgggatcgt
      421 tcggatcgtt ctcgcgcgac acgatggtct gcaggccgag gacgatgcgc gcggcgatga
      481 cgaccggatc gatcgcggtt tgcggatagg cgccgtggcc tcccttgccg tacaccgtca
      541 tgtcgagacc ggtcgagttg gcgcggaagt accccgagtg gaacccgatc acgccggccg
      601 gcagggaagg ctcgtcgtga atgcccagcg catagtcggg tttcgggaag cgcgtaaaca
      661 aaccatcgcg cagcattgcc gtcgcgccag ccacgacctc ctcggccggt tgcccgacca
      721 gcatcagcgt gccatgccag tgcgcgcggt tcttcgccat gagctcggcc gtgccgctcc
      781 acgccgccat gtgcacatcg tgtccgcaca tgtgcgacac cgacacgacg tcgccggcat
      841 cgttcttcgt cttcaccgtg ctcgcgaagg gcaggcccgt cttctcctcg atcggcaacg
      901 catcgagctc cgtgcgtaac atcaccaccg gcccgggtcc gttcctgagg atggcgacga
      961 caccggtgcc accgacgccg gtcgtgacct cataccccaa cgctttgacg cgcgccgcca
     1021 gcgtcgccgc cgtgcgctgc tcgtgaagcg agagctccgg attctggtgc agatcgatgt
     1081 agagcttctc gatatcggga tagatcgcat cgacctgcgc cgcgccgatg ccgtcgcctg
     1141 ggcgcgacgt ttgcgcggat gctgtggagg cgaacaacag cgtcgcgaac aggagtgtcg
     1201 cgatcaacaa gtttgcggga gtcgtaggat gtcgcgtgtc gggggtcgta gcagaagccc
     1261 aggttctcga tttcttgacg tggtccatgc ttttctccga tgtctgtaca gtgttcaggt
     1321 ccgactggaa ctgcttgctc atgccaactg tcggagaggg cgtgcgcgac gcgaaccgca
     1381 ggagccaccg cgggctcggg tagtagcgat gagcaggtcc gtgccggctc tcctcgccag
     1441 taacggatgt ttattgccct tgtggcggct gccagagctc aaccttgttg ccctcgggat
     1501 ccatgaccca tccgaatttc ccgtactccc cgtcgtcggt cttctcgagc acattgcaac
     1561 cttcctcgcg cagtgctttg agaagtgctg tcagatcttc gacgcggtag ttgaccatga
     1621 aggccgcctt gctcggagcg aagtgcttgc cgtccgctgg accgatcgac caggccgtag
     1681 ttcccttggt cgggttgcct tcggcgtccg tccagtcgaa ggctgctcca ccccagggct
     1741 gaacgtcgat cccgaggtgt ttcttgtacc aatcgcatag tgccttggga tcctgggcgc
     1801 tcatgaagat gccgccgatg cctgtgactc ttcgcattcg gacctccgat gttgtttgaa
     1861 gcgtgcaagg gtagcactcc cggccggccc gaattgtttc catgctggcg cagactgcgc
     1921 taacaagccc taccttctcg gctcataacg catcgccacc gctcccgagc ggagctccag
     1981 ccggctcacg agcttcaggt cgatacgctt cgacagcccc gcgaacaacg ccggcccgtg
     2041 gcccgctatc ctcgggtgta ccacgagctc gtattcatca atgagaccga gctccgccag
     2101 cgccatcggg agcttcacgc ctcccgtgaa cagtccctta cccggctccg tcttgagccg
     2161 ctgaacggcc gtggccagat ccccgcgcac cagctccgca ttccaatcga cccgctccag
     2221 ggtgctcgac acgacgtact tctttgccgc gtcgatcgtc cgggcgaagg gctccatcca
     2281 atcgggcctc gctccagtcc ccgccggcgg ccgccacgct gcctccatca tttcgtatgt
     2341 cacccggcca aagaggaggg catcagcctg agcgagattc tcgaccgcat gacgatgcaa
     2401 gtcctcgtcc gcgaacgctt cacgatgatc gcagcatccg tctagtgtga cgttgatgga
     2461 gtaccgaagg ggacgcatta cctgagagta ccgcccatgc ggtggacggg caaggtcagc
     2521 ccgtttcagg gctggacccc gcgccccggc tgaagcgggg gcgagggatc tgacgtcatg
     2581 tcatgcgcgc aagcgccagt tgcgttggag tgcgatcagt gcgcagcggg cagcggtttc
     2641 ttcggcgcaa cgacacagat ggtttgtcct ttcgtcttca cgtcatcgac tttgctgtac
     2701 atcttctgaa ccgtccaggt attagcaccg accgtgatct gtccgggttc gccgttgcag
     2761 atgtctccat tctcagagcc gccgtcgtca tcgaaccacg ccgtgagctt cgggtcggtt
     2821 agcatctccg acacttcgtg agtcgttacc tgcgtctgcc gctgctcgcg cgtctggccg
     2881 acttggagag aacattgtgt cggatcggga cacgtgttgt ggatgcacgc gtcgttgagg
     2941 cttggaatca ccgcgtagaa gaagtcgtgc cccttcgagg tcttgaagaa gaagtgatat
     3001 ccgaaggcgt catcactcgt gggctcacac atggtcgtgt cggcgtcctt caccccgatg
     3061 ccatcatcga ggaagatcac caggcaatcg ttcggcccgg gctccatgat tgtgccgtcg
     3121 tcgatggcct tctgaatgat ggtgtgaatg tccttgtcgg agatctctgc ggccacagcc
     3181 gccaccgacg tttcgcccgc aaacgcgcca tgtccgacac cgtactgcgt gaggatgttc
     3241 atgtacttgc ttgcgaggaa atcgcgcatg aactgattga ggcggtcggc gcgagcacgg
     3301 ttcggtggtg tgctccaacc ggctccccag aacgatgtcg tgatcctggg cgcgctaatg
     3361 acgggcccgc catgattctt gaaatttggg tggcgggtac caacgcccgc cgctgtatga
     3421 atccgcttgt gcggcgccac tccacgcatt gccttcgtct cagaactcat accggtacct
     3481 ccgtgcataa ggacggacgc gccccagttt ttccgagcgg acacaaggag atgagatgag
     3541 aaggccaacc cccgcgcagt aatcttcagt gtgaaagcac agaggatctg cagaaaagga
     3601 gattggccat gaagaagtct accaagagcg tgagca
//
LOCUS       DATLVW010000023        21218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_208012, whole genome shotgun sequence.
ACCESSION   DATLVW010000023 DATLVW010000000
VERSION     DATLVW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208012"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(468..1979)
                     /locus_tag="VNN25_00695"
     CDS             complement(468..1979)
                     /locus_tag="VNN25_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH90067.1"
                     /translation="MDLKSGIAIEDIADGGMLSGEVGDEKLVLVRHGKEVFAVGAECT
                     HYHGPLAEGIVAGETIRCPWHHACFDLRTGAPSAPALNPIAVWNVEQRDGRVFVTTKR
                     DIPPSPRAQTTVNSVGIVGAGCAGNAAAETLRREGFEGSITMIGAEETVPIDRPNLSK
                     EYLAGSAPEDWMPLRGTDFYRDQKIDLLTGRRALQLDAKTKTLTLDDGSKRDFDAILI
                     ATGSEPVRLSPPGANRIHLLRTLRDSRAIIAEAVKGKRAVVIGASFIGLEVAASLRAR
                     EVEVTVVAPDQVPLGKTMGVEVGAFIRRLHEEHGVHFRLGTTVATFEGDRVVLATGEP
                     IAADFVVAGVGVRPNTQLAQSAGLTIDNGIVVDEFLQSSAPGIFAAGDVAHYPDPFTG
                     NKIRVEHWVAAGRQGQTAARNILGRRTPHTAPPFFWSAHYDVTIAYVGNGAGFDKVEI
                     HGSLDERRALAAYHAGSKIIAVAAIGLDRESLLIEKAMEAGDRSAIDEVIKAI"
     gene            complement(1982..2566)
                     /gene="folE"
                     /locus_tag="VNN25_00700"
     CDS             complement(1982..2566)
                     /gene="folE"
                     /locus_tag="VNN25_00700"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXH90068.1"
                     /translation="MHEAKRNEVLEFPTDLAASEAVTTILRFIGEDPSRDGLADTPGR
                     VLKAWREMTSGYGDDPAEILARTFDETSDEMVVLRNISFHSTCEHHMLPFYGEVSVGY
                     LPGKVVGISKLARLVDCFARRLQIQERLTRQIADSIEEHLEARGVGVIVRAHHLCMGC
                     RGVRQQTTEMVTSSMLGTLRSDPMARAEFLRLTE"
     gene            complement(3286..3870)
                     /locus_tag="VNN25_00705"
     CDS             complement(3286..3870)
                     /locus_tag="VNN25_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXH90069.1"
                     /translation="MKFRTLAVAAFLVTASSLFAETFNVDKAHSEATFQVRHMMSKVS
                     GRFDDFSGKINADRANPAASSVEFTMKTASINTGNADRDKDLRSANFFDADKNPEITF
                     KSTKIVASKKKDLYEVTGDLTMHGVTKRVTLPVEFNGFGKDPWGGERAGFSLTTTLNR
                     KDYGVNWNKALDNGGVLLGDDVTININIEAAKAK"
     gene            complement(3867..4388)
                     /locus_tag="VNN25_00710"
     CDS             complement(3867..4388)
                     /locus_tag="VNN25_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXH90070.1"
                     /translation="MTTKVPAMAKSIVQELKQTRPFPSKAQEVAVGLMRTADVLRRLV
                     GSIVEPKGITLQQYNVLRILRGAGKDGLPTLDIAERMIETAPGITRLIDRLETKKLVS
                     RERCPTDRRQVFCRITKSGLTLLDKLDAPMIEAADSALGHLKKHELASLIDLLDRTRE
                     HANRQFTERRRSQ"
     gene            complement(4421..5242)
                     /gene="ispE"
                     /locus_tag="VNN25_00715"
     CDS             complement(4421..5242)
                     /gene="ispE"
                     /locus_tag="VNN25_00715"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXH90071.1"
                     /translation="MTQQKLITEIPAYAKINWSLRITGRRADGFHDLETLFQTISLHD
                     TLTIRESDHFSLTCDDPSIPVDDSNLVLRAARALGAPPVAIELKKAIPAGGGLGGGSS
                     DAAAVLVALSKHFGIDKPLADLALTLGSDVPFFLAGGTAYATGRGEVLTPMPSVAGIP
                     LLLLIPEERVSTAGAFAALRRFSPPIGIDRYRSIVKDGLLSHTNELVNDFEEPIFAQL
                     PRLRELKIRLIEAGAAWAAMSGSGSTIVGAFTGAEDRDAAIGRFNDVRAVAAETK"
     gene            complement(5373..6041)
                     /locus_tag="VNN25_00720"
     CDS             complement(5373..6041)
                     /locus_tag="VNN25_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90072.1"
                     /translation="MRRSLVVLVLCAIAAGCRTTRPAGEETPLNPLTSTSANDAARQL
                     AERRAQMTSQRSLVMIRATTAGHSLSLRAQLQIDNGGRMLLTAYTPFGTNAIRLYAEG
                     GQVTFVNDIEGTWWHGGVAEFGRSFGFFGSASPSSIGLLLMGLPASADFTYEYAATGL
                     KRANAGDVVVVYDPPSYPPPHILLTHGDQSLDIQTLESATTTAAIVPPAIPSDYRCCF
                     VPKM"
     gene            complement(6043..7959)
                     /locus_tag="VNN25_00725"
     CDS             complement(6043..7959)
                     /locus_tag="VNN25_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH90073.1"
                     /translation="MKPAKKIAVLLLMLLAFSAAAWAQVPSDDAYEFILAKLAADDDK
                     LDEALSRIDKIIAKDPQNTVVLFERAMILVDASKMDAAETELRKVVALQPNFFDAERV
                     LGRVLLDRAGTDRAKVDEALAHLQNAFRINPDDLATGMAISQIYQQTGRPAEAEKVIA
                     AMLERAPDQRGLNYSYALVLTKLGRGAESLKYLERAVEVDPTFGPAIQQLVEIYQSTN
                     QWQKAADVLQPLINDDPTNLDFQRQQAYFYLRAGQSEKARSRFAALLAADPKDTRTQF
                     YLAESLSDLERYEDAEKIYRQLLEKTPDDPDILVSFGLSQMGRKKYDDATKTFKTLLA
                     VPDVPEGAVTIAKTQLALIDLQKGNFQAAIDAVKPLFIFHDKPNPQPINIAVDALKKQ
                     KRYSDAVALLQPLADKYGSDPFVNARYIEMLARAGDKDRARVAAATQSKFGVRNSISA
                     AEAYVQAEQYEPAIGVLKDALRTKPDDVDLQFELGAVIERSGDKAGAEKMFTEILEKN
                     PDNGSVLNYLGYMYAEQGVHLDRAATMLTKAVELEPHNGAYLDSLGWTYFRQGKLELA
                     EKFLNDAAQILPRDATVHEHLGDVLAKRGDANRALSLYRVALTLDPESKDEAKLRSKI
                     AELEKQQQAQQTPR"
     gene            8122..9570
                     /locus_tag="VNN25_00730"
     CDS             8122..9570
                     /locus_tag="VNN25_00730"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH90074.1"
                     /translation="MSLERIEITPFAVIAGEILQQRRSGSLTIVQARLRKTLYWAQGE
                     LALITSAAPEDSLADFLVRRGVVSPAQALQLATQNPSEAVSAFHESGLFDMSKRQTFL
                     REWLTLQFAQLFSLEEGTAAFTDEQAIEPEKRVFLQSTAAVVLDGIRAITNGLVLRRS
                     LGDLSRTIEPDPAEQSLLDAIPLTDAERKLAEALTKPQTIESFLKQFPNDSLQAARVV
                     IAMMTLGVFVVADETRTQAAAIAEESDTQRDLEILAALGPNDQRSLRAVAFSRQLASI
                     DHYQLLDVPRAASRAQIIAAAEEKKGQYSPSKYPAVVKDAVTAISKRIDEAASTLEHP
                     ARRQAYDKLLHQDPGSGGGSEDLQRRAVRHSIARQNVAHAKDLSAAGDYYGAIILLKQ
                     AVAYKPDDAEAWHLLGACQERNPKWRRDSAESFQRSLSLDPNSIDTLISLGDLYRGEG
                     LTARAQSCYEDALKIDPEHAEAKGRLAAITKR"
     gene            9573..10208
                     /locus_tag="VNN25_00735"
     CDS             9573..10208
                     /locus_tag="VNN25_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH90075.1"
                     /translation="MKTFTMTFAFLCCVSLAGSARAVTVDDWRRDQAAIEAQLQQKQY
                     AAARKASIKLTNQMLDRLGGNAEAIRLLADTVSLRASAEEGLGNADDAKWYQQVAAAI
                     DPPRKALAKDPDSPLARAVFSSPAIKAPEVIHRQQPVRPGIIDAIGPSKVTVKVVIDI
                     DGVVRQPAIVESPAPSTSYAALEAVRQWRFRPGTSDGKPVPVIFSLTVTFH"
     gene            10354..10869
                     /locus_tag="VNN25_00740"
     CDS             10354..10869
                     /locus_tag="VNN25_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90076.1"
                     /translation="MSSQLEWARLVVEVLKGIAWPLLVLTLALVYRRPLYALLSNIGG
                     IAHRAVTDSFELSLGEKLRVAFRESISDANPQTVAEAIAVAEENADKALTLLATLKSV
                     PLSHYERRFLFQAAEEAAREGSWTPAYPDARAQRAIGSLVTKGLLEKDESGAYRVHPL
                     VAHYVLNSYLE"
     gene            complement(11008..11190)
                     /locus_tag="VNN25_00745"
     CDS             complement(11008..11190)
                     /locus_tag="VNN25_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90077.1"
                     /translation="MSEDEKGATEADMQKIDVIRGAESLGYEGAGREEHSGENSPKRP
                     NNAQDELADNKPEDIR"
     gene            complement(11307..12365)
                     /locus_tag="VNN25_00750"
     CDS             complement(11307..12365)
                     /locus_tag="VNN25_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXH90078.1"
                     /translation="MAESTLKLPNEAAVPKENRLGLKAADYQGGKSTLCAGCGHDAVS
                     NQIVRAFYEMNVKSEMVAKFSGIGCSSKTPAYFLGRAHGFNSVHGRMPAVATGAMLAN
                     HHLISIGVSGDGDTASIGLGQFMHMVRRNIPMIYIIENNGVYGLTKGQFSATADEGSK
                     LKTGVVNDLPPMDLCALAVELGCGFVARGYAGDPKQMVNILKAAIAHDGLAVIDVISP
                     CVTFNNHDGSTKGYTYSKEHDELLHQIGFVQSYEQIEIEYEPGTMREVTLHDGSRLLL
                     KKLEEDYDPSSAVQALQRSHKAIEDGAMLTGLIYLRPEKKSFISLLNMSEKSLCLLTE
                     AETRPPKEALDQIMRELM"
     gene            complement(12369..14273)
                     /locus_tag="VNN25_00755"
     CDS             complement(12369..14273)
                     /locus_tag="VNN25_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXH90079.1"
                     /translation="MSDSMTATAVLDEPQAGVASIVNDCTIHVATVNGSGSQSSNNVL
                     MRSIFQMGVPVSGKNMFPSNIAGLPTWFTIRANKDGWIARKREVDIMVCMNAATSRED
                     VEALQPGSVCIYDAPLNCKAIRSDITFFEVPFAKLAAELSQDSRLRKLLTNMIYVGIV
                     AERLGIERDEIMKALTKQFKGRKKLIDPNVAAIDKGLDYARENLPSQHRWRIERMHAT
                     EGKIIIDGNAAAAIGAMFGGCTVVTWYPITPSSSLCESLIDYMKEHRIGPDGKATFAI
                     VQAEDELAAMGMVLGAGWAGARSLTSTSGPGISLMAEFAGLGYFAELPGVIWDIQRVG
                     PSTGLPTRTAQGDIFEVYYLSHGDTQHIALIPASVSECFDFAGEALDLAEMFQTPIFV
                     LSDLDLGMNNWMSDPFVYPTKAYRRGKVLDAKQLDELGGKWGRYADVDDDGIPFRTLP
                     GTDHLMASYFTRGSGHDAQARYSEKPSDYKATVDRLAKKHQTAKKFVPQPVIDRRPDA
                     TIGFIAFGTTDPALEESRDQLRREHGVETSYLRLRALPFTDDVKAFVESHERVYVVEQ
                     NRDGQMGDLIRLEVGDIQDRIRKILHYTGLPCDARSITNSLLQMESDGTRVIRVGLEG
                     RTTTQTASED"
     gene            complement(14502..15287)
                     /locus_tag="VNN25_00760"
     CDS             complement(14502..15287)
                     /locus_tag="VNN25_00760"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXH90080.1"
                     /translation="MSTTRRTLVLLVALALAACTRRSQFLEREVHLGDHTYKYRVWLP
                     HHYTKLHRWPVILYLHGSGEQGIDNQLQISEGLGPAVERFGERYKAVVVFPQCDPAHE
                     WYGEMELMTMAELNAAVREFHGDSRRIYLTGISMGGAGAWYMARHNRTFAAIVPIAGD
                     VVRAPDDPFPSDPPPDIARIVGAPDPYTTLAGMIGSTPVWAIHGALDPVVPVIESRSM
                     VTALRKAGNNARYTEYIQGEHNVWDAAYANPELVHWMLQQKLK"
     gene            complement(15288..15995)
                     /locus_tag="VNN25_00765"
     CDS             complement(15288..15995)
                     /locus_tag="VNN25_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH90081.1"
                     /translation="MGISAIIAAYNEEKTIAEVLSVLTKSSLIDEIIVVSDGSSDRTV
                     EIARSFDVTTIALRENRGKGYAMRVGVDYARHDILFFVDGDMFNVTDEHIESLLRPVI
                     RNECDMNIGVRNRGPIRNFFHLQMKVGPVLSGIRVMRRSVWDTVPLRYQERFKIEAAL
                     NFFCSRAGYRQRQTIIYDLGHLIKENKRGLLPGLTARWEMSHEVVLLHWDLYVLETWR
                     SDVEYEPVPLAEYDLFE"
     gene            16225..16725
                     /locus_tag="VNN25_00770"
     CDS             16225..16725
                     /locus_tag="VNN25_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HXH90082.1"
                     /translation="MKRTLITATLLTACCIVLAAVTLSLSGCRSLLPGEIVNPSYRLR
                     SIKPHVAIALPLTRSAMDFDFTIVVDNPNPIGVHLEYLDFDLAINDTPILHSVRADQG
                     VNIPAHGVGDIHLRTRVEYSNLKALFREIADMVQGNRASYRIEGNAYYRTPFGLKRFP
                     VTLYSR"
     gene            16826..18043
                     /locus_tag="VNN25_00775"
     CDS             16826..18043
                     /locus_tag="VNN25_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH90083.1"
                     /translation="MATDRQPSRLFAASVAGLLYFSEGLPFGIVNDLLPVYLRVHHVE
                     LTTIGFLSTVASAWTLKVFWSPLIDSFGTYRRWIATAVLAMAICMATMATLPAAIGAT
                     FWTVITIFAIASATQDLAVDAFTIRATPPELVGPVNSIRVAAYRAALIVGGGGLVALS
                     GVIGWRGSFAAAAVIAGVVFVVALRLPDDRGARDERKSIVELFRGLGHWLARPRAGFL
                     LAIVLLYRLGELAIYAMIKPYWVDRGYSPAEIGTITAVIGVIVSILGAIAGGAIVARI
                     GLYRAMLWLGAAQILSNLGYAIVATTHAGRWAIYVAAIVENIGYGLGTGAFLAFLMAI
                     CDRERAATEYAMLTAAFGLTRVVIGTPSGWIAQNAGYPAYFWLTVVLGIPGLLLVPFV
                     REQLAAKQAVVSS"
     gene            18252..18935
                     /locus_tag="VNN25_00780"
     CDS             18252..18935
                     /locus_tag="VNN25_00780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXH90084.1"
                     /translation="MKHRFFVDADLIAGSAVPLSRDERHHAHVVRVREGEEVEVFNGR
                     GAAFVAKYSPELLQLTAPAPDREAHAGLHLAMAIINLDKFDLVLQKATELGVRSIIPL
                     VTERVEIRAERYRGKAERWRKIVFEAVKQSGRSVIPVVEEPAPFLDVVRREGRKLVFD
                     ADAEPSAWRPEDPATLFIGPEGGWSEREIAEARENGCDFERLGIRRLRAETAAIVATA
                     LIAARYADI"
     gene            18972..20261
                     /locus_tag="VNN25_00785"
     CDS             18972..20261
                     /locus_tag="VNN25_00785"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HXH90085.1"
                     /translation="MPTNSLTITDNRTGKTYEVPISEETIRATDLRKIKVADDDFGLM
                     TYDPAFMNTAACRSAITFIDGDKGILEYRGYPIEQLAEKSSYLETSWLLLKGELPTKD
                     ELAAWTHTITYHTFINENLKGVLDSFRYNAHPMGMLIAMISALGTFYNEAKEIFDDGA
                     RNRQIERLIAKMITIAGFGYRHMMGMPYVYPDNNLSYPGNFLNMMFKTTELKYEPHPA
                     LERALDVLFILHADHEQNCSTSTMRVVGSSHVDPYSAVAAACAALYGPLHGGANEQVI
                     RMLTEIGSKDRIPAFIEKVKGGEGVKLMGFGHRVYKNYDPRATIIKKVAYEVFEVTGT
                     SPLLDIALELERIALEDEYFVKRKLYPNVDFYSGLIYQALKFPMDMFPVLFAIPRVSG
                     WLAQWCEMLNDADQKIARPRQIFTGAARRDYKGIEQR"
     gene            20281..21177
                     /locus_tag="VNN25_00790"
     CDS             20281..21177
                     /locus_tag="VNN25_00790"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXH90086.1"
                     /translation="MPALLETSLPLPLARRGKVRDVYDLGDSFLFVATDRISAFDVVL
                     SPGIPDKGKILTQMSTFWFRQFGDIENHLVETEWNAFPAEVRAHAELRGRSVIVRKCK
                     VVPVECVARGYLVGSGLKEYKETGKVCGIELERGLTTASRLPEPIFTPATKEETGHDE
                     NISFERMCTIVGDDLATQLRDLTLRIYQRAAAYAETRGIIIADTKFEFGIDEHATRGN
                     IVVWIDEALTPDSSRFWPADKYAVGSNPPSFDKQFVRDWLETTGWDKASTPPELPEDV
                     VMKTREKYIEAFRELTGAEPDL"
BASE COUNT         3857 a         6429 c         6790 g         4142 t
ORIGIN      
        1 cgtgcagtgt aagccgggtg gtttgcagtg cgagccgggc ggcgtgcagt gtaagccggg
       61 tggtttgcag tgcgagccag gcggcgtgca gtgtcagccg ggcggtttgc agtgtaagcc
      121 gggtggcttg cagtgtaagc cgggtggttt gcagtgtaag ccgggtggtt tgcagtgcga
      181 gccggacggt ttgcagcgta agccgggagg attgcagtgt gaaccgggcg gcttgcggtg
      241 tcagccgggc ggcttgcggt gtcagccggg cggtttgcgg tgtcagccga gcgttttgcg
      301 ctgtcagccg ggcggtttgc ggtacgcacc cagtaggcgg cggagacgcc gcctggccag
      361 ccggcgggac gccggcgttc cgccggcgca aagtgcgctt ctggcggatg gaacgctggc
      421 gtcctcgccg gctggctcgg cggcgtctcg ccgccgtggc cgcggcctca gatcgccttg
      481 atcacctcgt cgatcgcgct gcggtccccg gcttccatcg ccttctcgat cagcagcgat
      541 tcgcggtcga ggccgatggc ggctactgcg atgatcttcg agccggcgtg gtaggcggca
      601 agggcgcggc gctcatccag cgagccgtga atctcgacct tgtcgaagcc ggcgccgttg
      661 cctacatagg cgatcgtcac gtcgtagtgc gcgctccaga agaacggcgg cgcggtgtgg
      721 ggtgtgcggc ggccgaggat gttgcgagcg gcggtttggc cctggcggcc ggcggcgacc
      781 cagtgctcga cgcggatctt gtttccggtg aaggggtcgg gatagtgggc gacgtcgccg
      841 gcggcgaaga tgccgggggc gctgctttga agaaactcgt cgacgacgat gccgttgtcg
      901 atcgtcaggc cggcggattg ggcgagctgc gtgttcggac ggacgccgac gccggcgacg
      961 acgaagtcgg cggcgatcgg ttcgccggtg gcgagaacga cgcggtcgcc ttcgaacgtg
     1021 gccacggtcg tgccgaggcg gaagtggacg ccgtgttctt cgtggagacg gcggatgaat
     1081 gcgccgacct cgacacccat cgttttcccg agcgggacct ggtcgggcgc gacgacggtg
     1141 acttcgactt cgcgcgcgcg cagcgacgcg gcgacttcga gtccgatgaa gctggcgccg
     1201 atgacgacgg cgcgcttccc cttcaccgcc tcggcgatga tggcccggct gtcgcgcagc
     1261 gtgcgcagca ggtgaatgcg attggcgccc ggcggcgaca ggcggacagg ctcggagccg
     1321 gtggcgatga ggatcgcgtc gaagtcgcgt ttcgatccgt cgtcgagcgt gagcgttttc
     1381 gtcttcgcat cgagctgcaa ggcgcgacga ccggtgagaa gatcgatctt ctgatcgcga
     1441 taaaagtccg taccgcgcag aggcatccaa tcctcgggtg cgctgcccgc gagatactcc
     1501 ttcgagagat tcggccggtc gatcggaacg gtctcctccg cgccgatcat cgtgatcgag
     1561 ccttcgaatc cttcgcggcg cagcgtctcg gctgctgcgt ttccggcaca gccggcgccg
     1621 acgatgccga ccgaattgac ggttgtctgc gcccgggggg atgggggaat gtcgcgcttc
     1681 gtggtgacga agacccggcc atcgcgttgc tcgacgttcc agaccgcgat cggattcaac
     1741 gccggggcgc tcggagcgcc ggttcgcaga tcgaaacagg cgtgatgcca gggacagcgg
     1801 atcgtctcgc cggccacgat gccctcggcc agcggaccgt gatagtgcgt gcattccgcg
     1861 ccgaccgcga aaacttcctt cccgtggcgg acgagaacca acttttcatc acccacttcg
     1921 ccggaaagca tgccgccgtc ggcgatgtct tcgatcgcaa tgccggattt cagatccata
     1981 gtcactccgt aagccgcagg aactcggcgc gcgccatggg gtcgctgcgc agcgtgccca
     2041 gcatcgacga cgtcaccatc tccgtggtct gttgccgcac gcctcggcat cccatgcaca
     2101 ggtgatgcgc acggacgatg acgccgactc cgcgggcctc gagatgctct tcgatcgagt
     2161 cggcgatctg ccgcgtgagc cgctcctgga tctgcaggcg gcgggcgaag cagtcgacca
     2221 gccgggccag cttgctgatg ccgacgactt tcccgggcag atagccgacg ctcacctcgc
     2281 catagaacgg cagcatgtga tgctcgcagg tggagtgaaa actgatgttg cgcagcacga
     2341 ccatctcatc gctcgtttcg tcgaacgtgc gcgcgaggat ctcggccgga tcgtcgccgt
     2401 agccggatgt catctcccgc caggctttga gcacgcgtcc cggggtgtcc gcgaggccgt
     2461 ctcgggaggg atcctcgccg atgaaccgga gaatggtggt tacagcctcg gacgcagcca
     2521 gatcggtggg gaactccagc acttcgttac gtttagcctc atgcatcgaa tacctcctcg
     2581 tgtggcaggg caacgatctc atgaacggtt gtgtttagca agtgtttgct tgctaaacat
     2641 acgcgaatta cgaacccatg cgtcacccaa gcaagaacgc tacggatttc gacctccgcc
     2701 ctgacagcac tcgggcgagg acgcccgagc gcctgcggtc gagacgaccg cactccttgc
     2761 gctgaggagt gtgggcgtct cgcccgcggc catcggggcg tcccgcctcg gtggttggat
     2821 cggcctgata actccaaggt ttgaaacagg tccaggtctg aacagccgca ggacaaagct
     2881 agagtttgcg cggcagagac tacgaacccg tacttcaccc aagcaagagt gctacggatt
     2941 ttttagacct ccgctctgac agcacccggg cgaggacgcc cgagtgcccg cggtcgagac
     3001 gaccgcactc cttgcgctga ggagtgtggg cgtctcgccc gcggccatcg gggcgtcccg
     3061 cctcggtggt tggatcggcc tgatgactcc aaggttctaa acagacccag gtctcaacag
     3121 ccgtaggacc acgctagagt ttgcgcggca gagactacga actcgtacta cacccaagca
     3181 agaacgctac ggattttcga cctccgcact gacagcactc gggcgaggac gcccgagtgc
     3241 ccgcggtcga gacgaccgca ctccaaggag tgtgggacca acaagctact tcgccttcgc
     3301 cgcctcgata ttgatgttga tcgtgacgtc gtcgccgagg aggacgccgc cgttgtcgag
     3361 ggctttgttc cagttgacgc cgtagtcctt gcggttgagg gtcgtcgtca gggagaagcc
     3421 ggcgcgctcg ccgccccacg gatccttgcc gaagccgttg aattcgaccg gaagggtgac
     3481 gcgcttcgtc acaccgtgca tcgtcaggtc gccggttacc tcgtagaggt ccttcttctt
     3541 tgacgcgacg atcttcgtgc tcttgaaggt gatctcgggg ttcttgtcgg cgtcgaagaa
     3601 gttcgcgctg cggagatcct tgtcgcgatc ggcattgccg gtgttgatcg aggcggtctt
     3661 catcgtgaac tcgaccgatg aggcggccgg gttggcgcgg tcggcgttga tcttgccgct
     3721 gaagtcgtcg aacctgccgc tgactttgct catcatgtgg cgcacctgga acgtggcctc
     3781 ggaatgcgct ttgtcgacgt tgaacgtttc ggcaaagagc gaggacgcgg taacgagaaa
     3841 tgctgcaact gcgagggtac ggaacttcat tgggaacgtc tcctttcggt gaactgccgg
     3901 ttggcatgct cgcgggtcct gtcgagaagg tcgatgaggc tcgcgagctc gtgttttttc
     3961 agatggccga gggcggaatc ggcggcctcg atcatggggg cgtcgagttt gtcgagaagg
     4021 gtgagaccgg acttggtgat gcgacagaag acctggcggc gatcggtggg gcagcgttcg
     4081 cgcgacacga gtttcttcgt ctcgagccgg tcgatgaggc gcgtgatgcc gggcgcggtc
     4141 tcgatcatcc gttcggcgat gtcgagcgtc ggcaggccgt ctttcccggc gccgcgcaga
     4201 atcctgagga cgttgtactg ctggagggtt atgcccttcg gctcgacgat cgaaccgacc
     4261 agccggcgaa gaacgtccgc ggtgcgcatg agaccgacag caacctcctg cgccttcgac
     4321 ggaaagggcc tggtctgctt caactcctga acgatgctct tcgccatggc cggtacttta
     4381 gtggtcatta gttgattagt caagtattcg gcacgcgctt ttatttcgtc tccgctgcga
     4441 ccgcccggac atcgttgaat cgcccgatcg cggcatcgcg gtcctccgcc cctgtgaatg
     4501 caccaacaat ggtcgatccg gagccggaca tcgctgccca ggccgctccc gcctcgataa
     4561 gccttatctt cagctcgcgc agacgcggga gttgcgcgaa gatcggctct tcgaaatcgt
     4621 tgacgagctc gttggtgtgc gagagcaatc catctttgac gatcgatcga taacggtcga
     4681 tgccgatcgg tggcgagaaa cggcgcaagg cggcgaaggc gcctgccgtg ctgacgcgct
     4741 cttccgggat cagcaacaga agcgggatgc ctgcaacgga cggcatcggc gtcagcactt
     4801 caccgcggcc ggtggcgtag gcggtgccgc cggcgaggaa gaaaggaacg tccgagccga
     4861 gggtgagggc aagatcggca aggggcttgt cgatgccgaa gtgcttggac agcgcgacga
     4921 gaacggcggc ggcgtcggat gatccgccgc cgagaccgcc gccggcggga atcgccttct
     4981 tcaactcgat agcgacggga ggggcgccga gggcgcgcgc ggcgcggagg acgagattgc
     5041 tgtcatcgac aggtatcgac ggatcgtcgc aggtcagcga gaagtggtcc gactcgcgga
     5101 tcgtcagcgt gtcgtgcagc gagatggtct ggaagagcgt ctcgagatca tggaacccgt
     5161 ccgcccgcct gccggtgatg cgcagcgacc agttgatctt cgcgtacgcg gggatctcgg
     5221 tgatcagttt ttgttgtgtc atccgcctgc tcagaatgac acaacgcaat tggaaaaagc
     5281 cgtttcacca cagagacaca gaggtcacag agatcctctc tgtgttctct gtgtctctgt
     5341 ggttgccttt gcttcggcga ccatccgcgc cctcacatct tcggcacgaa gcagcatcgg
     5401 tagtccgagg gaatcgcggg ggggacgatc gcggcggtcg tcgtcgcgga ctccagcgtc
     5461 tggatatcga gactctgatc gccgtgcgtc aggaggatat gtggcggcgg ataggacggc
     5521 ggatcgtaga cgactacgac atcgccggcg ttggcgcgtt tgaggccggt ggctgcgtat
     5581 tcgtacgtga agtcggcgct cgccgggagt cccatgagca gcaggccgat cgacgatggc
     5641 gatgcggagc cgaagaagcc gaagctgcgt ccgaactcgg ccacgcctcc gtgccaccac
     5701 gtcccttcga tgtcattgac aaacgtcacc tggccgcctt cggcgtagag gcggatcgca
     5761 ttcgtgccga acggcgtgta ggcggtcagg agcatgcgtc cgccgttgtc gatttggagt
     5821 tgggcgcgca acgacagcga atggcccgcg gtcgtcgcgc gaatcatgac cagactgcgc
     5881 tggctggtca tctgcgcgcg gcgctccgca agctggcgcg cggcgtcatt cgccgacgtc
     5941 gatgtcagcg gattgagcgg cgtctcctcg cctgccggac gcgtagtcct gcaaccggct
     6001 gcgatggcgc aaagcaccag aacgacaagc gagcgccgca tctcagcgtg gagtctgctg
     6061 cgcctgctgc tgcttttcga gctcggcgat cttggagcgc agcttggcct cgtccttcga
     6121 ctccggatcg agagtcaagg ccacgcggta aagcgagagc gcgcggttgg cgtctccccg
     6181 cttggcgaga acgtcgccga ggtgctcgtg caccgtggca tcacgcggca ggatctgcgc
     6241 ggcatcgttc agaaacttct cggctagctc gagcttcccc tggcggaagt aggtccagcc
     6301 gagggagtcg agataggcgc cgttgtgcgg ctcgagctcc accgccttgg tcagcatcgt
     6361 ggcggcgcgg tcgaggtgaa cgccctgctc ggcgtacatg tacccgaggt agttcaggac
     6421 gctgccgttg tcaggattct tctccaggat ctccgtgaac atcttctcag caccggcctt
     6481 atcgccggag cgctcgatca ccgcgccgag ctcgaactgg agatcgacat catcgggctt
     6541 cgtgcgcagc gcgtctttga gcacgccgat ggcaggctcg tactgctccg cctgcacgta
     6601 cgcttccgcg gcggagatgc tgttgcgaac gccgaacttc gactgcgtgg cggcagcgac
     6661 gcgggcccgg tctttgtcgc cggcccgcgc cagcatttcg atgtaacgcg cgttgacgaa
     6721 tggatcggac ccgtacttgt cggcgagcgg ctgcagcagc gcgacggcgt cgctgtagcg
     6781 cttctgcttc ttcagcgcgt cgacggcgat gttgatcggc tgggggttcg gtttgtcgtg
     6841 aaagatgaag agcggcttga cggcatcgat ggccgcttga aagttgccct tctgcagatc
     6901 gatgagcgca agctgcgtct tggcaatggt caccgcgccc tcgggtacat ccggaactgc
     6961 cagcagcgtc ttgaacgtct tcgtcgcatc gtcgtacttc ttccggccca tctgcgacag
     7021 gccgaagctg acgagaatgt cgggatcgtc gggcgtcttc tcgagaagct gccggtagat
     7081 cttctcggca tcctcgtagc gctccagatc actcagcgac tcggcgagat aaaactgcgt
     7141 gcgcgtgtct ttcggatcgg ctgccaacag tgcagcgaaa cggcttcgag ccttttccga
     7201 ttgaccggca cggagataga agtaggcctg ctgccgctgg aaatcgaggt tggtgggatc
     7261 gtcgttgatg agcggctgta gaacgtcggc cgccttctgc cactgattcg tgctctggta
     7321 gatctcgacg agctgttgga tagcggggcc gaaggtcgga tcgacctcga cggctcgctc
     7381 cagatatttg agggactcgg cgccgcggcc gagcttcgtg agaacgagcg cgtagctgta
     7441 gttgagtcct cgctggtcgg gcgcacgttc cagcatcgcg gcgatgacct tctccgcctc
     7501 ggcaggccgt ccagtctgtt ggtaaatctg cgagatggcc atgccggtgg cgagatcatc
     7561 gggattgatg cggaaagcgt tctggagatg ggcgagcgcc tcatcgactt tggcgcggtc
     7621 ggtaccggcg cggtcgagca gaacacgtcc gagaacgcgc tcggcatcga agaagttcgg
     7681 ctgcaaggcg acgactttgc gaagctccgt ttccgcggcg tccatcttcg acgcgtcgac
     7741 caggatcatg gcgcgctcga agaggacgac cgtattctgc ggatctttgg cgatgatctt
     7801 gtcgatgcgg ctcagcgcct cgtcgagctt gtcgtcatcg gcggcaagtt tggcaaggat
     7861 gaattcgtac gcgtcgtcgg aaggtacctg ggcccacgcc gccgcgctga acgccagtag
     7921 catcagcagc agaacggcaa tcttttttgc tggtttcatc atcacctcgg agcacactcg
     7981 gcagaacgga acaacgtcaa cggacggggc attctggcgg cattgtccgt cttgcaacag
     8041 cattgcgacg aagccggacg gagcccgttt catgccacat ttgtgctaaa agcagagcct
     8101 taatcgaatt gaggatgcgg cgtgagtctg gagcgtatcg agatcacacc cttcgctgtg
     8161 atcgcggggg aaatactgca acagcgccga agcgggtccc tgaccatcgt tcaggcccgc
     8221 ctgcgcaaaa ctctgtattg ggctcagggc gagctggctc tgatcacgtc tgccgcgcct
     8281 gaagactcgc tggccgactt cctcgtccgc cgcggcgtcg tctcccctgc ccaggcgttg
     8341 cagctcgcga cgcagaatcc ctcggaggcc gtgtcggcgt ttcacgaatc cggccttttc
     8401 gacatgtcga aacggcagac gttcctgcgc gagtggctga cgctgcagtt cgcgcagctt
     8461 ttctctctcg aagaagggac cgcagcattc accgacgaac aggccatcga gccggagaag
     8521 cgggtcttcc tgcaatcgac cgccgccgtg gttctcgatg gcatccgggc gatcacgaat
     8581 ggcctcgtgc tccggcgctc gctcggcgat ctatcccgca cgatcgagcc cgatccggcg
     8641 gagcaatcgc tgctcgatgc gatcccgctg accgatgcag agcgcaagct ggcggaggcg
     8701 ctcaccaaac cgcaaacgat cgaatcgttc ctgaaacagt ttcccaacga ttcgctacag
     8761 gctgcccgcg tcgtgatcgc gatgatgacg ctgggagtct ttgtcgtcgc cgacgagacg
     8821 cgtacgcaag cggctgccat cgctgaagag agcgacacgc aacgcgacct cgagatcctc
     8881 gcggcgctcg gaccgaacga tcagcgctcg ctgcgcgcgg tggcgttctc gcggcaactg
     8941 gcctcgatcg atcattacca gttgctcgat gtgccgcgtg cggcgtcgcg cgcacaaatc
     9001 atcgccgccg cggaagaaaa gaaggggcag tactcccctt caaagtatcc ggcggtggtg
     9061 aaggatgccg tcaccgcgat cagcaaacgc atcgatgaag ccgcaagtac tctcgaacat
     9121 ccggccagac gccaggccta cgacaagctt ctccatcaag acccgggttc cggcggcgga
     9181 agcgaggatc tacagcgacg cgcggtacgg cactccatcg cccggcagaa tgttgcgcat
     9241 gcaaaggact tgagcgcggc gggcgactac tacggcgcga tcatcctgct caaacaggcg
     9301 gtcgcgtaca agcccgatga cgccgaggcg tggcacctcc tgggggcgtg tcaggagcgg
     9361 aatccgaagt ggcgccgcga ctccgcggaa agctttcagc gctccctctc cctcgacccg
     9421 aattccatcg acaccctcat ctccctcggc gacctttacc gcggcgaagg cctgaccgcg
     9481 cgcgcgcaaa gctgctacga agacgcgctg aagatcgatc ccgaacacgc ggaggcgaag
     9541 ggacggttgg cggcgatcac gaagaggtga cgatgaagac tttcacgatg acttttgcgt
     9601 tcttgtgctg cgtatctctg gcagggtccg cccgagccgt caccgtcgac gactggcgcc
     9661 gcgatcaggc ggcaatcgaa gcgcaactgc agcagaagca gtacgccgcg gcacgaaaag
     9721 catcgatcaa gctgaccaat caaatgctcg accgcctcgg gggcaacgcc gaagctatcc
     9781 ggctgctggc cgacacggta tcgctgcgcg catccgcgga agaaggcctc ggcaatgccg
     9841 atgacgcgaa gtggtatcag caagtcgccg ccgcgatcga tccgccgaga aaggcgttgg
     9901 cgaaagaccc tgattcaccg cttgcacgcg cggtcttttc atcgcccgca atcaaagcgc
     9961 ctgaagtgat ccaccgccag cagcctgtgc ggcccggaat catcgatgca atcgggccgt
    10021 cgaaggtgac cgttaaagtc gtcatcgata tcgacggcgt ggtccgccaa ccggcaattg
    10081 tggagtctcc cgctccttct acgtcctacg ccgcgttaga agcagtccgt caatggcgct
    10141 tccgccccgg aacgtcggat ggcaagccgg tgccggtgat tttcagtttg acggtgacgt
    10201 ttcactgagg gccggtcgtc cagccgcgcg aagcagtcag cgcgcgcatc gaggcagaga
    10261 tgccacgagt gctcctgatg aacagtcctg cgggcagtct tgcgcgcctc tgcttagttg
    10321 ggattcggat acactttgta ccagcagtga accatgtcct cgcaactgga gtgggcacga
    10381 cttgttgtcg aagtactcaa gggcattgcg tggccgctgc tcgtgctgac acttgcactc
    10441 gtttaccggc ggccactgta cgcgctgctc agcaacatag gggggattgc tcaccgcgca
    10501 gtcactgact ccttcgagct atcgcttggc gaaaagctac gtgtcgcatt tcgagaatcg
    10561 atctcggacg ccaatcctca aacagtcgct gaggcaatcg ccgttgccga ggaaaacgcc
    10621 gacaaggcgc tgacgcttct cgcgacactc aagagcgtac cgttgtctca ttatgaacgg
    10681 cgctttctct tccaggcggc agaagaggca gcgcgtgagg gtagctggac gccagcctac
    10741 ccagatgccc gcgcgcaacg agcaatcggt agccttgtaa caaaaggatt gctagagaag
    10801 gacgagagcg gcgcataccg agttcatcct ctcgtcgctc actacgttct caattcctat
    10861 cttgaatgag tccaccagcg tattcggtca gactgtacga cctgtcgctc gtcagccgca
    10921 atgaggtatg agcaatagcc gacgtttaca gatcgcaacc acacacggtt gggctcgctc
    10981 tactttggaa aacttgcgtt agttcgccta gcggatatct tcgggcttgt tgtccgctag
    11041 ttcgtcctgc gcgttgttcg gacgctttgg ggagttctcg ccgctgtgtt cttcgcggcc
    11101 ggcgccttcg tagccgaggg attcggctcc gcggatgacg tcgattttct gcatgtcggc
    11161 ttcggtggcg cctttttcgt cttcgctcat ggcgtattgc tgagcaagag gcgggccggg
    11221 tctcacgcga aggcgcaaaa gccgcgaaga gagcatcgct aattcccctt cgcgtcttcg
    11281 cgtcttcgcg tgagaagtct tagcagctac atcaactcgc gcatgatctg gtcgagcgct
    11341 tccttcggcg gacgcgtttc ggcttcggtg agcaggcaga gtgacttttc gctcatgtta
    11401 agcaggctga tgaagctctt tttctcgggg cgcaggtaga tgaggccggt gagcatggcg
    11461 ccgtcttcga ttgctttgtg cgagcgctgg agcgcttgca cggcgctcga cgggtcgtaa
    11521 tcctcttcca gctttttcag caggaggcgc gagccgtcgt gcagggtgac ttcgcgcatc
    11581 gtgccgggct cgtactcgat ctcgatctgt tcatacgact gcacgaaacc gatctggtgg
    11641 agtagttcgt catgttcttt cgagtatgtg tagcccttcg tcgagccgtc gtgattgttg
    11701 aacgtgacgc acggcgagat gacgtcgatg acagcaagac catcgtgcgc gatggcggct
    11761 ttgaggatgt tcaccatctg cttcggatcg cctgcgtagc cccgggcgac gaaaccgcat
    11821 ccgagctcga cggccagcgc gcagaggtcc atcggcggga ggtcgttgac gacgccggtc
    11881 ttgagcttcg agccttcgtc ggcggtcgcg gagaactgcc ctttggtcag gccgtacacg
    11941 ccgttgttct cgatgatgta gatcatcggg atgttgcgcc ggaccatgtg catgaactgg
    12001 ccgagtccga tggaggcggt gtcgccgtcg ccggaaacgc cgatcgagat gagatggtga
    12061 ttggccagca tagcgccggt ggcaacggcc ggcatgcgtc cgtgaacgct gttgaatcca
    12121 tgggcacggc cgagaaaata cgccggcgtt ttcgacgaac agccgatgcc ggagaacttc
    12181 gccaccatct ccgacttgac gttcatctcg taaaacgcgc ggacgatctg attgctgacg
    12241 gcgtcgtggc cgcagccggc gcagagcgtc gacttgccgc cctggtaatc ggccgctttc
    12301 agtccgagcc ggttttcctt cggtacggcc gcctcgttgg gcagtttgag agtactttcg
    12361 gccatgagct agtcctccga ggcagtctgc gtcgtcgtgc ggccttcgag accgacgcga
    12421 atgacgcgcg tgccgtcgct ttccatctgc aacagtgagt tcgtgatcga gcgcgcgtca
    12481 cagggcagac ccgtgtagtg aagaatcttg cggatcctgt cctgaatgtc gcccacttcg
    12541 agccggatca gatcgcccat ttgtccatcg cgattctgct cgacgacgta gacacgttcg
    12601 tgcgattcga cgaacgcttt cacatcgtcg gtgaacggca gcgcccgcag acgaaggtag
    12661 ctcgtctcga cgccgtgctc ccggcggagc tgatcgcgcg attcttcgag cgcagggtcg
    12721 gtggtaccga aggcgatgaa gccgatcgtc gcatcgggcc ggcgatcgat caccggctgc
    12781 ggcacgaact tcttcgcggt ctgatgcttt ttcgcgagcc ggtcgacggt ggctttgtag
    12841 tcggaaggct tctccgagta gcgcgcctgc gcgtcgtgtc ccgaaccgcg cgtgaagtac
    12901 gacgccatca agtgatcggt gcccggaagc gtacggaacg gaataccgtc gtcgtcgacg
    12961 tcggcatagc gtccccactt tccgccgagc tcgtcgagct gtttggcgtc gagcaccttg
    13021 cctcggcgat acgccttggt cggatagacg aagggatccg acatccagtt gttcatgccg
    13081 aggtcgaggt ccgagagaac gaagatgggt gtttggaaca tctcggcgag atcgagcgcc
    13141 tcaccggcga agtcgaagca ctccgacacc gacgccggaa tcagagcgat gtgctgcgtg
    13201 tcgccgtgcg agaggtagta aacctcgaag atgtcgccct gcgcggtgcg cgtgggcaac
    13261 ccagtggacg gaccgacgcg ctggatgtcc cagatcacgc caggcaattc ggcgaagtat
    13321 ccgagtccgg cgaactccgc catcagcgag atgcccggcc ccgaggtgga ggtcagcgag
    13381 cgagcgcccg cccatcccgc gcccaacacc atccccatcg ccgccagctc atcctcggcc
    13441 tgaacaattg caaacgtcgc ttttccatcc ggcccgatgc gatgctcctt catgtaatcg
    13501 atgagcgact cgcagagcga tgacgagggc gtgatcggat accacgtgac gacggtgcag
    13561 ccgccaaaca tggcgccgat cgccgccgcg gcgttgccgt cgatgatgat cttgccttcc
    13621 gtcgcgtgca tccgctcgat gcgccagcga tgctgcgacg gcaggttctc acgcgcgtag
    13681 tcgagccctt tgtcgatggc ggcgacgttc ggatcgatca gtttctttcg gcctttgaac
    13741 tgcttggtga gcgccttcat gatctcgtcg cgctcgatgc cgaggcgctc ggcgacgata
    13801 ccgacgtaga tcatgttcgt gagcagcttg cggaggcgcg agtcctgcga gagctcggcg
    13861 gccagcttcg cgaacggcac ttcgaagaag gtgatgtcgg agcggatggc cttgcagttg
    13921 agaggcgcgt cgtagatgca gacggagccc ggctgcagcg cttccacgtc ttcgcgcgac
    13981 gtcgcggcat tcatgcagac catgatgtcg acttcccgct tgcgcgcgat ccagccgtct
    14041 ttgttcgccc ggatcgtgaa ccaggtgggg agcccggcga tgttcgaggg gaacatgttc
    14101 ttcccgctca ccggcacgcc catctggaag atcgagcgca tcaggacatt gttcgatgac
    14161 tgcgatccgg agccgttgac ggtggcaaca tggatggtgc agtcgttgac gatggaagcg
    14221 acgccggcct ggggctcatc gagcaccgcc gtcgcggtca tgctatcgga cacgtctttc
    14281 tcctttgact ggtggattga gaagaaggag gcgggaaccg cgggctgtca ttttggaagg
    14341 gggcgattgt agcgttagaa gggtgaagag tgaagtgaga gatcggagac gaagagagca
    14401 gagagcagag actagagatc agagatcagg aatcgggcgc tggtcggacg ttttgttcga
    14461 tcccctgatc tctgatctct cgtctctgat ctctgaatct ctcacttcag cttctgctgc
    14521 agcatccagt gaaccagctc cggattggcg tacgcggcat cccacacgtt gtgctcacct
    14581 tgtatgtatt cggtgtagcg ggcgttgttg cccgccttgc gcagcgcggt gaccatcgag
    14641 cgtgactcga tgaccggaac caccgggtcc agcgcaccgt gaatcgccca caccggcgtg
    14701 ctgccgatca tcccggcgag ggtggtgtag ggatcgggcg cgccgacgat gcgggcgatg
    14761 tcgggcggag gatcgctggg gaacgggtcg tcgggagctc gcaccacatc gcccgcgatc
    14821 ggaacgatgg ccgcaaacgt ccggttgtgc cgcgccatgt accaggcgcc ggcacctccc
    14881 atcgaaatgc ccgtgaggta gatacggcgg gagtcgccgt gaaattcacg aacggcggcg
    14941 ttgagctccg ccatcgtcat cagctccatc tctccgtacc actcgtgcgc gggatcgcac
    15001 tgcggaaaga cgacgacggc cttgtaccgt tcgccgaagc gctccacggc cgggcccaac
    15061 ccttcgctga tctgcagttg attgtcgatg ccctgctcgc cgcttccatg cagatagagg
    15121 atgaccggcc agcggtggag tttggtgtag tggtgaggaa gccagacgcg gtacttgtag
    15181 gtgtggtcgc cgaggtggac ttcgcgctcg agaaactgcg aacggcgcgt gcaggcggcg
    15241 agggccagcg ctacgaggag gacgagagtg cggcgcgtgg tgctcactta ctcgaacaga
    15301 tcgtactcgg cgaggggcac cggttcatat tcgacgtcag aacgccacgt ttccagaacg
    15361 tagaggtccc aatgaaggag aaccacttcg tgcgacatct cccatcgcgc ggtgaggccg
    15421 gggagaagtc cgcgcttgtt ctctttgatc aagtgcccga ggtcgtaaat gatggtctgg
    15481 cgctgccggt aaccggcgcg cgaacagaag aagttgagag cggcttcgat cttgaagcgc
    15541 tcctggtaac ggagcggaac cgtgtcccac acactgcgcc gcatcacgcg aatgcccgag
    15601 agtaccggcc cgactttcat ctgcagatgg aagaagttgc ggatcgggcc gcggttgcgg
    15661 acgccgatgt tcatgtcgca ttcgttccga atcacgggac gcagcagcga ttcgatgtgc
    15721 tcgtcggtga cgttgaacat gtcgccgtcg acgaagaaga ggatgtcgtg gcgcgcgtag
    15781 tcgacgccga cacgcatggc gtagcccttg ccgcggttct cgcggagagc aatggtcgtg
    15841 acatcgaagg agcgtgcgat ttcgacggtg cggtcggacg agccgtcgct gacgacgatg
    15901 atctcgtcga tgagactgct cttggtcaga acgctcagaa cctcggcgat cgtcttctct
    15961 tcgttgtacg ccgcaatgat ggctgagata cccaactagc tctcctacgc tccgctcgcc
    16021 ggaatggact gcaaccggca gaccatacca cacggttttc gggttccggc ggttcggaat
    16081 cggacggaaa tcagagatca gagacatcgg agatcagaga tcaggacgca atctggtctc
    16141 atgtctctga tctctggtct cttgtctctg gtctctggtc tctgacttca ggcggccaga
    16201 ccattgcata gaatgccgtt tgcgatgaaa cgaacgctga ttaccgcaac gctcctcacg
    16261 gcctgctgca tcgtcctggc ggcggtgacg ctctcgctct ccggatgccg gtcgctgctg
    16321 ccgggtgaaa tcgtcaatcc cagctaccga ctacggtcca tcaaaccgca cgttgcgatc
    16381 gcacttccgc tcaccagatc ggcgatggac ttcgacttca cgatcgtcgt cgataatccc
    16441 aacccgatcg gcgtgcacct cgagtatctc gacttcgatc tcgccatcaa cgacacgccg
    16501 attctgcaca gcgtgcgcgc cgatcagggc gtcaacatcc ccgcgcacgg agtgggcgac
    16561 attcatctgc gcacccgcgt ggagtattcg aacctgaagg cgctcttccg cgagatcgcc
    16621 gatatggtgc aaggcaatcg ggcgagctac cggatcgagg ggaacgcgta ttaccgcacg
    16681 ccgttcgggc tgaagcgatt cccggtgacg ttgtactcgc gttagttttt tttaccacag
    16741 aggcacagag gtcacagaga aaacctctgt gttctcggtg tctctgtggt tcagtctttt
    16801 cgctctcgat tcataatgct cgcgcttggc caccgaccgt cagccctcgc gattgttcgc
    16861 tgcttcggtg gccggacttc tctacttttc ggaagggctc cccttcggca tcgtcaatga
    16921 ccttcttcct gtctatctgc gcgttcatca cgtcgagctc acgacgatcg gattcctcag
    16981 cacggtggcc tcggcctgga cactgaaagt cttctggtcg ccgctgatcg attcgtttgg
    17041 aacgtatcgg cgctggatcg ccacggcggt cttggcgatg gcgatctgta tggcgacgat
    17101 ggcgacgctg cctgccgcga tcggcgccac gttctggacc gtcatcacga tcttcgccat
    17161 cgcctcggcc acgcaggatc tcgccgtcga tgctttcact attcgcgcga cgcctccgga
    17221 gcttgtcgga cccgtgaact ccattcgcgt ggcggcttat cgcgcggcgc tcatcgtcgg
    17281 aggaggggga ctcgtggcgc tctccggcgt catcggctgg cggggcagct tcgcggccgc
    17341 cgccgtcatc gccggagtcg tgttcgtcgt cgcgctgcga ttgcccgatg accgaggggc
    17401 gcgtgacgag cgcaaatcga tcgtcgagct ctttcgcggg cttgggcact ggctggcccg
    17461 tcctcgtgcg ggctttctcc tcgccatcgt gcttctctac cgcctcggcg agctggccat
    17521 ctacgcgatg atcaaaccgt actgggtcga ccgcggctat tcgcccgcgg agatcgggac
    17581 gatcaccgcg gtcatcggcg tcatcgtcag tattctcggc gcgatcgcag gcggcgcgat
    17641 cgtggcgcgc atcggtctct atcgcgccat gctctggctt ggtgctgccc agattctctc
    17701 caacctcggg tacgcgatcg ttgccacgac ccacgccggc cgctgggcca tctacgtcgc
    17761 agccatcgtc gagaacatcg gctacggcct cggcaccggc gcattcctgg cgtttctcat
    17821 ggccatctgc gaccgcgaac gcgccgcaac ggagtatgca atgctcaccg ccgccttcgg
    17881 cctgacgcgc gtcgtcatcg ggacaccgag tggatggatc gcccaaaacg ccggctaccc
    17941 ggcatacttc tggctgaccg tcgtgcttgg gattccagga ctgctgctgg tgccgttcgt
    18001 gagggagcaa ctcgcggcga agcaagcagt cgtgtcatcc tgaaccggcg aagcgcggtg
    18061 aaggaactca aaatgcgacg atcacggcag cccagcggag cgaacgcatt caagccatgc
    18121 tctgcgtatt ctgagatcct tcgccgtcct gcgccgccag gtcgtgctgc gcgagggctc
    18181 ggacagcgag ggcggctccg gcaggctcag gatgacaccg cgcgcacatc gccctgcagc
    18241 gcggctctcc catgaaacac cgcttcttcg tcgacgcaga tctcatcgct ggttccgcgg
    18301 ttccgctgag ccgcgatgag cggcatcacg cgcatgtcgt gcgggtgcgc gaaggggaag
    18361 aggtcgaagt gttcaatggg cgcggcgcgg cgttcgtcgc gaagtactcg ccggagcttc
    18421 tgcaattgac ggctccggcg cccgaccgtg aagcgcacgc cggtctgcac ctggcgatgg
    18481 cgatcatcaa tctcgacaag ttcgacctcg tgctgcagaa ggcgacggag ctcggcgttc
    18541 gctcgatcat cccgctcgtc accgagcgcg tcgagattcg cgccgagcga tatcgcggca
    18601 aggcggagcg gtggcgcaaa atcgtcttcg aagcggtcaa gcaatccggc cggtcggtca
    18661 tccccgtcgt cgaagagccg gctccgttcc ttgatgttgt ccggcgagag ggacggaaac
    18721 tcgtcttcga tgccgatgcg gagccgagcg cctggcggcc ggaagacccg gccacgctct
    18781 tcattggccc cgagggggga tggagcgagc gggagatcgc ggaggcgcgg gagaatggtt
    18841 gcgatttcga acggctcgga attcgcaggc ttcgcgccga gaccgcagcg atcgtcgcta
    18901 ccgcgttgat cgctgcgcga tatgccgaca tttgatagat ttgcgcgctc gattgtcacc
    18961 ggaggagtct tttgccaacc aacagcctca cgattaccga taaccgtacg ggcaaaacgt
    19021 acgaagtacc gatcagcgaa gagaccattc gcgccaccga cctgcgcaag atcaaggtcg
    19081 ccgatgacga cttcgggttg atgacatacg acccggcttt catgaacacc gcggcgtgcc
    19141 gaagcgccat caccttcatc gacggcgaca aaggcatcct cgagtatcgc ggttatccga
    19201 tcgagcagct cgcggagaag agcagctacc tcgagacctc atggctcctg ctcaaaggcg
    19261 agctccccac caaagatgaa ctggctgcgt ggacgcacac gatcacgtat cacaccttca
    19321 tcaacgagaa cctcaaaggc gttctcgatt ccttccgcta caacgcccat ccgatgggca
    19381 tgctcatcgc catgatcagc gccctcggca ccttctacaa cgaggcgaag gagatcttcg
    19441 atgacggtgc cagaaaccgt cagatcgaac ggctcatcgc caagatgatc accatcgccg
    19501 gattcggcta ccggcacatg atggggatgc cctacgtcta ccccgacaac aacctctcct
    19561 atcccggcaa cttcttgaac atgatgttca agacgacgga gctgaagtac gagccgcatc
    19621 cggccctgga gcgcgcgctc gacgttctct tcatcctcca cgccgatcac gagcagaact
    19681 gttcgacgag cacgatgcgc gtcgtcggca gctcgcacgt cgatccgtat tcagccgtcg
    19741 ccgccgcatg cgccgcgctt tatggaccgc tccacggcgg cgccaacgag caggtcatcc
    19801 gtatgctgac ggagatcggg tcgaaagaca ggatcccggc tttcatcgag aaggtgaagg
    19861 gtggcgaagg cgtgaagctg atgggcttcg gccaccgcgt ctacaagaac tacgaccctc
    19921 gggcgacgat catcaagaag gtcgcctacg aggtgttcga agtgacggga accagtccgc
    19981 tgctcgacat cgcgctggag ctggagcgca tcgccctcga ggatgagtac ttcgtcaagc
    20041 gcaagctcta cccgaacgtc gacttctact ccggcctgat ctaccaggcg ctgaagttcc
    20101 cgatggacat gttccccgtc ctcttcgcca ttccgcgcgt ttccggctgg ctggcgcagt
    20161 ggtgcgaaat gctgaacgat gccgatcaga agattgcgcg cccgaggcag atctttaccg
    20221 gagctgcgcg gcgggattac aaggggatcg agcagagata gagcctcaga ccagactccc
    20281 atgcctgcgc tactcgagac cagcctcccc ctcccactcg cccgccgcgg caaagtgcgc
    20341 gatgtctacg acctcggcga ttcctttctc ttcgtcgcca ccgaccgcat ctccgcattc
    20401 gatgtcgtcc tttcgccggg gatccccgac aagggaaaga tcctcacgca gatgtcgacg
    20461 ttctggttcc gccagttcgg cgacatcgag aatcacctcg tcgagaccga gtggaacgcg
    20521 ttcccggctg aagtgcgggc gcacgccgaa cttcgcggac ggagcgtgat cgtccgcaag
    20581 tgcaaggtcg tcccggtcga atgcgtggcg cggggctatc tcgtcgggtc aggtctcaag
    20641 gagtacaagg agaccggcaa agtctgcggc atcgagttgg agcgcgggct gacgacggcc
    20701 agccgcctgc ccgaaccgat cttcacgccg gcgacgaaag aagaaacagg ccacgacgag
    20761 aacatctcct tcgagcggat gtgcacaatc gtgggcgacg atctggccac ccaactacgc
    20821 gatctgacgt tgcggattta ccagcgcgcc gcggcatacg ccgaaacgcg cggcatcatc
    20881 atcgccgaca cgaagttcga gttcggcatc gacgagcacg ccacgcgcgg gaacatcgtc
    20941 gtctggatcg acgaagcact cacccccgac tcgtcccgct tctggccggc cgacaagtac
    21001 gccgtcggat cgaacccgcc atcattcgac aaacagttcg tacgcgactg gctggaaacc
    21061 accggctggg acaaagcctc cacacctccc gagcttcccg aggatgtcgt gatgaagacg
    21121 cgcgaaaagt acatcgaagc gtttcgagag ctgacgggtg cggagccgga tttgtagagc
    21181 cgttgtttaa ccacagagac acagaggaca cagagaag
//
LOCUS       DATLVW010000024         8852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_42989, whole genome shotgun sequence.
ACCESSION   DATLVW010000024 DATLVW010000000
VERSION     DATLVW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42989"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VNN25_00795"
     CDS             <1..369
                     /locus_tag="VNN25_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXH90087.1"
                     /translation="PLTVATMNAVHNIDPVHLAAARNFGIPLRARITRVLVPAILPQL
                     IVAMRIALGVAWLVVVAAEMIAVNSGLGFLIVDARNAGNRYDLVVAGMVLIGAIGIVL
                     DLLMRRIERLDSVRWGYAAE"
     gene            366..1100
                     /locus_tag="VNN25_00800"
     CDS             366..1100
                     /locus_tag="VNN25_00800"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH90088.1"
                     /translation="MKVQFSDISMRFGAMDVLQDVTIGVEEGEFLCIVGPSGCGKSTL
                     LNIAGGFLSPAKGTVTIDGDPVTGPDRRRIFVFQERGVFPWLTVEGNIGFGLFDLPDE
                     ERQRRVAQYVHLVGLEGFEKAYPRELSGGMKQRVEVARALAVNPDVLFLDEPFGALDS
                     ITRLQMRSELLRIWRAARKTVLFVTHDIEESVQLADRVVVMSARPGRIRRVVDIDIAH
                     PRDLSSRRYIELRDLIFGEIGLAHQV"
     gene            1119..1898
                     /locus_tag="VNN25_00805"
     CDS             1119..1898
                     /locus_tag="VNN25_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH90089.1"
                     /translation="MQRPTQHATSSIEHSTFEKLVLPLLTVAVIIAIWSAAVKFSGTK
                     IFPSPIAVASAIGTLGEKSLLWRYIADSLTRVFAGYAVAIVAGIPLGFLLGWNASLAR
                     AVNPLIQMLRPISPLAWMPLAVIWFGVSNAAPIFLIALASFFPIVVAAMNGVRNVPPM
                     YLQAGRNFGLSTWQLMIHVVIPAVLPRIIVGLRLAFGIAWVVLVAAEMIAVDSGLGYL
                     IIDARNAGKRYDLVVAGMLLIGVIGLLLDTGIRRVEEWISE"
     gene            2040..2189
                     /locus_tag="VNN25_00810"
     CDS             2040..2189
                     /locus_tag="VNN25_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90090.1"
                     /translation="MRKLVLAIGAWIIAITSAHFALNVNWQVLMNDRLPEGQRKLNVA
                     YIPVT"
     gene            2217..3101
                     /locus_tag="VNN25_00815"
     CDS             2217..3101
                     /locus_tag="VNN25_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH90091.1"
                     /translation="MSAYSQSGHLFIPRMFQGFPEIKEALMSNKMQAAFMVAPMAIAL
                     RAQGVPVKIVYLGHRYGSAVVVRRDGPVHRVSDLRGRIVAVPSRFSDERLIVFRAMAA
                     NGLDAKKDLRMVEMAPPDVAGALAAGAIDAFSMGEPYPSQAEIGGFGRVLFHARQYWP
                     DYISCVLVVRQDVIDKRPDAVQVLVDGIARSGLWLDEKERAKRHRMHAADFVGRFYYH
                     QDPKLLRWALTNPIDRVKYEPLSPRRADFETIVNLMVQTGVLDHAIPFEDFVDTRFAE
                     GAHAMTAWKYEAGDDRAR"
     gene            complement(3268..4044)
                     /gene="rsmA"
                     /locus_tag="VNN25_00820"
     CDS             complement(3268..4044)
                     /gene="rsmA"
                     /locus_tag="VNN25_00820"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXH90092.1"
                     /translation="MSHPKKKWGQHFLRNRGAVMRIVEAVEPEADDIVVEIGPGEGVL
                     TERLAELPNRLVAVEIDPELSERLRAQFGDRITLVNEDAIDAPLPAQPYRAVGNLPYN
                     AGTPILRRVVADPNCRRAVFMLQKEVADRLVAKPGDEAYGYLTLFVRLYASARILMTL
                     EPKSFYPPPKVRSAVVVLDPDRKPFVSDELIALISASFRMRRKKLVNNLLDLWTREEI
                     VAAMTSAGIDEGIRAERLSLEDFARLGSALGNEHPARSHP"
     gene            4117..5106
                     /locus_tag="VNN25_00825"
     CDS             4117..5106
                     /locus_tag="VNN25_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXH90093.1"
                     /translation="MDQATISSHIAAAHDVIARLRAEVGKAVIGQEAIVDRLVIALLI
                     RGHVLLEGVPGLAKTLLARTLAGAIDASFHRIQFTPDLLPADITGTLIFDPAGRAFKP
                     RQGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGDTSYRLPEPFIVIATQNPIE
                     HEGTYALPEAQVDRFVMKLRVGYPTQDEELRMLGIYLDPTAQQIGIQRILRIVEIEQI
                     AHLVASVHVDERISRYIIQLVSATRNPANFKLSSLVPHIEFGASPRATLALAHVAKGY
                     AFIQGRPYVIPEDVKAIAPDVLRHRLVLSYEAAAEQVTADAIVEQVLGAVAVP"
     gene            5132..6031
                     /locus_tag="VNN25_00830"
     CDS             5132..6031
                     /locus_tag="VNN25_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXH90094.1"
                     /translation="MNLFRRKKPILRPPLARRVRDLEVLSARLIRAGFAGDYHSAFHG
                     RGIEFSQVREYQPGDDVRTIDWNVTARSGAPHVKEFIEERDLTVLVAIDVSGSMAFGS
                     VDWRKCDIAAELAAVFAFSAAESSDRIGLLLFSNNVRRYIPPRRGRMHAQVIVRAAVD
                     AAMRGCEGIADLDAAARYLEHVSARRAVVIVISDFLDQNFERTMRRLNRRHDVVAIAV
                     GDPREDRFPEGGLARVIDAERGVQRLVDLRRAGVGGRAEARRTLIERRFRAAGIDHLT
                     VSTAIGYDHDLLRFFRERGIRRR"
     gene            6091..6927
                     /locus_tag="VNN25_00835"
     CDS             6091..6927
                     /locus_tag="VNN25_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90095.1"
                     /translation="MLALLLLTAITTFQPVRPTVGDPITIDFAAPTAVDPSPDYEVVS
                     RNGRQVVIRTFVPHTIAVTGKAADGPVSVMIPIHSVLKPDDKLEPAPLKPPKAEPWPR
                     MPFIAIGIAALCAIGAWTAVVLLARRRKPKMVVVVVPAELYRSRVLGLRRAAIGMRWA
                     SLADAVRSYLASVRSDLGAELTTKELLARVGEGEQAGEVAGEPPAEQPPGRRRYDGRG
                     YDARGVLGEILHQGDLEKFSPWGPAPADFDDAVNRALEIPLWIEPPAEPVAAPGEQEA
                     AA"
     gene            6924..7931
                     /locus_tag="VNN25_00840"
     CDS             6924..7931
                     /locus_tag="VNN25_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH90096.1"
                     /translation="MIRFANPLWLLLILAVAARIALVIRDRRKGFGSFTISSLSLVGP
                     KQSLRAQLAGVPFLLESLAALAMIVALSRPQRVTKMASNDRYGIDIVIALDASGSMAA
                     EDFKPRNRFGVAKELIGNFIERRQDDRIGIVTFGVRAATRVPITYDRDIAGAILERAE
                     VGENGDGTAIGHAIATCVNRLRTSKTRSRIIILVTDGVNNAGSIDPLTAAQLAARSGI
                     KIYTIGVGSRGPVPIPMKRQDPFTGEIVTTYQLVRADIDEETLAAIAKATNGEYFRAT
                     DAHTMGAVLDRIDHLEKTRLSAPKSERIEELYAAPLAAGIAALALALLAGETLWQKVT
                     A"
     gene            8125..>8852
                     /locus_tag="VNN25_00845"
     CDS             8125..>8852
                     /locus_tag="VNN25_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH90097.1"
                     /translation="MTAFTFRAPQLLWLALAVPLALFFFLARERTRTLVARRFVSERL
                     RGIANPLRSIRPWLVAVALLGCVGALAGPEMGFRVVPIEARETNRVIVMDVSLSMLAQ
                     DVGTSRLDAAKAIAKRIIDAQSGRVGLVIFESAAEVGSPLTSDGEAVEALLDSIQAGE
                     VSNPGSDISVALTAALRLVAGDPAQSGDIVVISDGEDQGTKLDETIAKLAQRGIPVST
                     ILVGTSGGSTIPRPEGGGELRDDSG"
BASE COUNT         1496 a         2751 c         2899 g         1706 t
ORIGIN      
        1 ccgctaaccg tggccacgat gaatgcggtg cacaacatcg atccggttca tctcgccgcc
       61 gcccggaatt tcggtatccc gctgcgggcg cggatcaccc gagtccttgt cccggccatc
      121 cttccgcagc tcatcgtggc catgcgcatc gccctcggcg tggcctggct cgtcgtcgtg
      181 gccgcggaga tgatcgcggt caactccgga ctcggctttc tcatcgtcga tgcccgcaac
      241 gccggcaacc ggtatgacct cgtcgtcgcc gggatggtcc tcatcggcgc gatcggcatc
      301 gtcctcgatc tcctgatgcg acgcatcgag cggctcgatt cggtccgctg gggttacgcc
      361 gccgaatgaa agttcagttc tccgacattt cgatgcggtt cggcgcgatg gacgtcctgc
      421 aggacgtgac catcggcgtt gaggaggggg agttcctctg tatcgttggc ccgtccggct
      481 gcggcaagtc gacgctcctc aacattgccg gcggcttcct gtcgccggcg aaggggaccg
      541 tgaccatcga cggcgatccg gtgaccgggc ccgaccggcg ccgcatcttc gtgttccagg
      601 agcgcggcgt cttcccatgg ctgaccgtcg aagggaacat cggattcggt ctcttcgatc
      661 tgcctgatga ggagcggcag cgccgtgttg cgcagtacgt ccacctcgtc ggcctcgagg
      721 gtttcgagaa ggcgtatcca cgcgagttgt ccggcggcat gaagcagcgc gtggaagtgg
      781 cgcgcgcgct ggccgtcaac ccagacgtgc tctttctcga tgagcccttc ggtgcgctcg
      841 attcgatcac gcgcctgcag atgcgcagcg agcttctgcg catctggcgt gccgcgcgca
      901 agaccgtgct gttcgtgacg cacgacatcg aagagtcggt gcagctcgcc gaccgcgtcg
      961 tagtgatgag cgcgcggccg gggcgtatcc gtcgcgtcgt cgacatcgat atcgcccatc
     1021 cgcgcgacct gtcgtcgcga cggtatatcg agctacgcga cctgatcttc ggggaaattg
     1081 gattggcgca tcaggtctga aacggaatga atgtgaccat gcaacgaccc actcaacacg
     1141 caacgtccag cattgaacat tcaactttcg agaaactcgt gctgccgttg ctcactgtgg
     1201 cagtcatcat cgccatctgg tccgcggcgg tgaagttctc cggcacgaag atctttccgt
     1261 cgccgatcgc cgtcgcgagc gccattggta cgcttggcga gaagagcctg ctgtggcgct
     1321 acatcgccga ttcgctcacg cgcgtttttg ccggctacgc cgtagccatc gtcgccggca
     1381 tcccgctcgg atttctcctc gggtggaacg catcgctggc ccgtgcagtc aatccgctga
     1441 ttcagatgtt gcggccgatc tccccgctgg cgtggatgcc gctggcggtg atctggttcg
     1501 gcgtcagcaa cgccgcgcca atctttctca tcgcactggc gtcattcttt ccgatcgtcg
     1561 tggcagcgat gaacggcgtg cgcaacgtcc cgccgatgta ccttcaggcc ggccgcaatt
     1621 tcggcctgtc gacgtggcaa ttgatgatcc acgtcgtcat ccccgcggtg ctgccgcgca
     1681 tcatcgtcgg cctgcgtctg gcgttcggaa tcgcctgggt ggtcctcgtt gccgccgaga
     1741 tgatcgccgt cgactccgga ctcgggtacc tcatcatcga tgcccgcaac gccggcaagc
     1801 gctatgacct ggtcgtcgcc ggcatgctcc tgatcggcgt gatcggcttg ctcctcgata
     1861 cagggattcg gagggtggag gagtggatta gtgagtaaag cagagatcag agattatcag
     1921 agatagagat caggagacag agatcaggga ctagagatca tagatcagga gtctcgggtg
     1981 cctttctggt ctctgatctc tagtctctga tctctgcttc aaaaggaggt gatcggaaca
     2041 tgcggaaact cgtccttgcc atcggcgcct ggatcattgc catcacatcc gcgcactttg
     2101 cgctgaatgt gaactggcag gtgctgatga atgaccggct gccggaggga cagcggaaac
     2161 tgaatgtcgc gtacatcccg gtaacgtgac atctggcttg cccggtcacc gactacatct
     2221 ccgcctactc gcagtccgga catctcttca tcccgcgcat gtttcaggga ttccctgaga
     2281 tcaaagaagc gctgatgtcc aacaagatgc aggcggcgtt catggtcgcg ccgatggcaa
     2341 tcgctctgcg ggctcagggc gttccggtga agattgtcta cctcggccac cgctacggtt
     2401 cggccgttgt cgtccgccgt gacggcccgg tccatcgtgt gtccgatctg cgcggacgga
     2461 tcgtcgctgt gcccagccgg ttttccgacg agcggctgat tgtcttccgg gccatggccg
     2521 cgaatggtct ggacgcgaag aaagacctgc gcatggtgga gatggcgccg ccggatgttg
     2581 ccggcgcgct agccgcgggc gcgatcgacg cgttctcgat gggtgagccg tatccgtccc
     2641 aggcggagat cggaggcttc ggccgcgtgc tctttcacgc aagacagtac tggcccgatt
     2701 acatctcctg cgttctcgtc gtccggcagg acgtgatcga caagcgcccc gatgctgtgc
     2761 aggtgctcgt cgacggcatc gcccgatcgg gactctggct cgacgaaaaa gagcgcgcca
     2821 aacgccatcg tatgcacgcc gccgatttcg tcggccgctt ttactatcac caggatccga
     2881 agctgctccg ctgggcgctg acgaatccga tcgaccgcgt gaagtacgag ccgctctccc
     2941 cccgtcgcgc cgacttcgaa acgatcgtca atctgatggt ccagaccggc gtgctcgatc
     3001 acgccatccc attcgaagac ttcgtcgaca cccgcttcgc cgagggcgcg cacgcgatga
     3061 cggcttggaa gtacgaggcg ggggatgatc gggcgaggtg aaaagccgct taccacagag
     3121 acacagagga cacagagtca ctctgtgctc tctgcgcctc tgtggttgat ggttttatac
     3181 acaccctaag acctcgcaac gccctccggc gactagacaa ccagcaggtc gagcttcctt
     3241 gaaatctcgt tgtccgatgc cgagatttca aggatgggat cgagccgggt gttcgttgcc
     3301 cagtgcgctc ccaagcctcg caaagtcctc aagcgacagc ctttccgcgc gaataccctc
     3361 atcgattccg gcagacgtca tcgcggcaac gatctcctca cgcgtccaga ggtcgagcag
     3421 gttgttgacc agtttcttgc ggcgcatgcg gaatgaggcg ctgatcagtg cgatcaactc
     3481 gtcggacacg aagggcttgc gatcggggtc gagcacgacg acggcgcttc gaacctttgg
     3541 cggcggatag aaagacttcg gctccagcgt catgaggatg cgcgccgagg cgtagagacg
     3601 gacgaagagg gtgaggtagc cgtaggcctc atcgccgggc tttgccacga ggcggtccgc
     3661 cacttctttc tgcagcatga aaacggcgcg gcggcaattg ggatcggcga cgacgcgtcg
     3721 caggatgggt gtgccggcgt tgtatggaag gttgccgacc gcgcggtagg gttgggcggg
     3781 gaggggagcg tcgatggcgt cttcgttgac gagcgtaatg cggtcgccga actgcgcgcg
     3841 aagacgctcc gacagctcgg gatcgatctc gacagccacg agccggttcg gcagctccgc
     3901 aagcctctca gtcagcacgc cctcgcccgg cccgatctcg acgacgatgt catcggcttc
     3961 gggctcgacc gcctccacga tgcgcatcac cgcgccgcgg ttgcggagga agtgctggcc
     4021 ccacttcttt tttggatgac tcattgctgg atacaacacg caacattcaa catttcgctt
     4081 ctaacccgcg tacgccctca cgataaactc tccgacatgg atcaggccac catcagttcg
     4141 cacatcgccg cggcgcacga cgtgattgcg cgcctgcgtg ccgaggtcgg gaaggcggtg
     4201 atcgggcagg aggcgatcgt cgaccggctc gtcatcgcgc tcttgattcg cggacacgtc
     4261 ctcctcgaag gcgttcccgg acttgccaag acgctgctcg cgcgcacgct ggccggtgca
     4321 atcgacgcgt cgtttcaccg tatccagttc acgcccgacc tgctgccggc cgacatcacc
     4381 ggcaccttga tcttcgatcc tgccggccgc gcgttcaagc cgcgtcaggg accgattttc
     4441 gcgaacctcg tgcttgccga cgagatcaat cgtgcacccg cgaaagtgca gtcggcactg
     4501 ctcgaggcga tgcaggaacg gcaagtcacg atcggcgata cctcgtatcg tcttcccgaa
     4561 ccgttcatcg ttatcgccac gcagaacccg atcgaacatg aggggacgta cgcgcttccg
     4621 gaagcgcagg tcgaccgctt cgtgatgaag ctgcgcgtcg gctatccgac gcaggacgaa
     4681 gagctgcgga tgctcggcat ctacctcgat ccaacggcgc agcagatcgg cattcaacgc
     4741 atcctgcgaa tcgtcgagat cgagcagatc gcgcacctcg tggccagcgt acacgtcgac
     4801 gagcggatca gccgctacat catccagctc gtcagcgcca cgcggaatcc ggcaaacttc
     4861 aagctctcct cgctcgtgcc gcacatcgag ttcggtgctt cgccgcgcgc aacgctcgcg
     4921 ctcgcgcatg tggcgaaggg ctatgcgttc attcagggcc ggccgtatgt gatccccgag
     4981 gatgtcaaag cgattgcgcc ggacgtgctg cgtcaccggc tggtcctcag ctacgaagcg
     5041 gccgccgagc aggtgaccgc cgatgccatc gtcgagcagg tgctcggcgc ggtggcggtg
     5101 ccgtgatggc ggtcgcgggg atctcgcgac catgaatctt ttccgccgca agaagccgat
     5161 tctccggcca ccgctggcgc ggcgtgtccg cgacctcgag gtcctgtcgg cgcggttgat
     5221 cagggccggg ttcgcgggcg actatcactc ggcgtttcac gggcgaggga tcgagttttc
     5281 gcaggtccgc gagtaccagc cgggcgatga cgtgcgcacg atcgactgga acgtcacagc
     5341 ccgcagcggt gcgccgcacg tcaaggagtt catcgaagag cgcgacctga ccgtgctcgt
     5401 ggccatcgac gtctccggct cgatggcgtt cggttcagtc gactggcgca agtgcgacat
     5461 cgcggcggag ctggcggcgg tcttcgcgtt ttccgccgcc gaaagctcgg accggatcgg
     5521 attgcttctc ttctcgaaca acgtacgccg ctacattccg ccgcggcgcg gcaggatgca
     5581 cgcgcaagtg atcgtccgtg ccgcggtcga tgcagcgatg cgcgggtgcg agggaatcgc
     5641 ggacctcgac gccgcggcgc gctatctcga acacgtcagc gcacgtcgtg cggtggtcat
     5701 cgtcatcagc gacttcctcg atcagaattt cgagcgcacg atgcgccgcc tcaaccgccg
     5761 ccacgacgtc gtggccatcg cggtgggcga tccgcgcgag gatcggtttc ccgagggagg
     5821 tctggcccgc gtcatcgatg cagagcgcgg cgtgcagcgg ctggtcgatc ttcgacgggc
     5881 tggagtaggt gggcgcgccg aggcgcgacg tacgctgatt gaaagacggt tccgcgctgc
     5941 cgggatcgac catttgacgg tgtcgaccgc gatcggatac gaccacgacc tcttgcgatt
     6001 cttccgcgag cgcggcatcc ggaggcgatg aatgccgatc gatgaatttg tgttcctcgc
     6061 tcgtcgttca tctttcgtcg ctcgatagag atgctcgcac ttctcctcct cactgcgatt
     6121 acgacgtttc agccggttcg gccgacggtc ggcgatccga tcacgatcga tttcgcggcc
     6181 ccgaccgcgg tcgatccatc gcctgactac gaggtggttt cgcgcaacgg ccggcaggtc
     6241 gtgatccgga cgtttgtgcc acacacgatc gcggtaaccg gaaaggcggc ggacggaccg
     6301 gtcagcgtga tgattccgat tcattcggtg ctgaagccgg atgacaagct ggagccggcg
     6361 ccgttgaagc cgccgaaggc agagccgtgg cctcggatgc cttttatcgc gatcggcatc
     6421 gcggcgctct gcgcgattgg ggcgtggacg gcggtggtcc tgctggcgag gcggcggaag
     6481 ccgaagatgg ttgtggtggt cgtgccggcg gagttgtatc gctcgcgggt gcttgggctt
     6541 cggcgcgcgg cgatcgggat gcgctgggca tctctcgccg acgcggttcg ctcgtatctc
     6601 gcctcggttc gttctgacct cggcgcggag ttgacgacga aggagttgtt ggcgcgggtg
     6661 ggggagggcg agcaagctgg tgaagttgcc ggcgagccgc cggcggaaca gccgccgggc
     6721 cggcggcgct acgatggacg cggctacgat gcgcgcggtg ttctcgggga gattcttcat
     6781 cagggggacc ttgagaagtt ctcgccgtgg ggaccggctc cggcggattt tgatgatgcg
     6841 gtcaatcggg cgttggagat cccgttgtgg attgagcctc cggccgagcc ggtggccgcg
     6901 cctggcgagc aggaggcggc ggcgtgattc gctttgccaa tccgctctgg ctgttgctga
     6961 tcctggccgt cgctgcgcgc atcgcgctgg tcattcgtga ccggcgcaag ggctttggct
     7021 cattcacgat ctcgtcgctc tcgctggtcg gtccgaaaca gagtctgcgg gcgcagcttg
     7081 cgggggtgcc gtttctgctg gagtcgctcg cggcgctggc gatgatcgtg gcgctttcgc
     7141 ggccgcagcg cgtgacgaag atggcctcga acgaccgcta cggcatcgac atcgtgattg
     7201 cgctcgatgc ctcgggcagc atggcggcgg aggacttcaa gccccgcaac cgattcggcg
     7261 tggccaagga gctgatcggc aacttcatcg agcggcggca ggacgaccgg atcggaatcg
     7321 tgacgttcgg tgtgcgtgcc gctacacgcg ttccgatcac gtacgaccgc gatattgccg
     7381 gcgcgattct cgagcgcgcg gaagtcggtg agaacggcga cggcacggcg atcggccacg
     7441 ccatcgcaac ctgcgtgaac cgtttacgca catcgaagac gcgcagccgg atcatcattc
     7501 tcgtcaccga cggggtgaac aacgcgggct cgatcgatcc gctcacggcg gcgcagcttg
     7561 ccgcacgctc cggcatcaag atctacacga tcggtgtcgg cagccgcggt ccggtgccga
     7621 ttccgatgaa gcgccaggat ccgttcacgg gcgagatcgt gacgacgtat caactcgtcc
     7681 gcgccgacat cgacgaggag acgctggccg caattgcaaa ggcgacgaac ggcgagtact
     7741 tccgggcgac ggacgcgcac acgatgggcg cagtcctcga ccgcatcgat catctagaaa
     7801 agacccgcct tagcgcaccc aagagcgagc ggatcgaaga gctctacgcc gcgccgctcg
     7861 ccgccggaat cgcggcgctt gcgctcgcgc tgctggcggg tgagacgttg tggcagaagg
     7921 tgacggcgtg agggcagaga tcagggaagg cagagatcag agagcaggag cctgcgcggg
     7981 tcgtcctcgt cggacctctg ttgccttgtg tctgaatcac cgatcagcga ctagagatca
     8041 gagatcagga gaccgcgccg gccaactcct cctggtctct gatctcttgt ctctaaccac
     8101 tcagaaccgc gggagcaaga tccagtgacc gccttcactt tccgcgcgcc ccaactcctc
     8161 tggctcgcgc tcgccgtccc tctcgcgctg ttctttttcc tggcgaggga gcgcacgcgg
     8221 acgctcgttg cgcggcggtt tgtttcggag cggcttcgcg ggattgccaa tccgctgcga
     8281 tcgatccggc cctggctcgt ggcagtggcg ctgctgggct gcgtgggcgc gctggcggga
     8341 ccggaaatgg gctttcgcgt cgttccgatc gaggcgcgcg agacgaaccg tgtgatcgtg
     8401 atggacgtct cgctcagcat gctggcgcag gatgtgggca cttcgcgtct cgacgcggcg
     8461 aaggcgatcg ccaaacgaat catcgatgcg cagtcgggac gcgtcgggct agtcatcttc
     8521 gagtcggcgg ccgaggttgg atcgccgctg acgagcgatg gcgaggcggt ggaggcactg
     8581 ctcgattcga ttcaggcggg cgaagtctcg aatccgggct ccgacatttc ggtggcgttg
     8641 actgcggcgc tcaggctggt cgctggcgat ccagcgcaga gcggcgacat tgtcgtcatc
     8701 agcgacggcg aggatcaggg cacaaagctc gacgagacga tcgccaaact ggcgcagcgc
     8761 gggattccgg tctcgacgat cctcgtcggc accagcggcg gaagcacgat tccgcgaccg
     8821 gaaggtggcg gcgaacttcg cgacgacagc gg
//
LOCUS       DATLVW010000025         6014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_43190, whole genome shotgun sequence.
ACCESSION   DATLVW010000025 DATLVW010000000
VERSION     DATLVW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43190"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VNN25_00850"
     CDS             complement(<1..623)
                     /locus_tag="VNN25_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH90098.1"
                     /translation="MLSVARAETQISFEALRAREFSRLDVQDLAYLDYAGSALYGESQ
                     LRAHHALLSEGVFGNPHSDSGPSHASTEIIDTARRRVLQFLDVDESTHDVCFTANTSA
                     AIKLVAESYPFQPESTFLLSADNHNSVNGIREYARRGGARVRYIPLDDELRLLDAQAI
                     IESESDRGGLLAFPAQSNFSGVRHPLAFVAKAKSRGFDVLLDLAAYVP"
     gene            complement(617..2071)
                     /locus_tag="VNN25_00855"
     CDS             complement(617..2071)
                     /locus_tag="VNN25_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HXH90099.1"
                     /translation="MNAVAPFFELSDHGPGAALIAAVAIGIAFGWTLERAGLGSAPKL
                     AGQFYFADLTVFKVMFSAIVTAMLGAFWLARLGMLDLSRVYVPETYLLPQLAGGLLFG
                     IGFVIAGLCPGTSCVAAATGRGDGLLVMFGMFAGVLGTGLAFGSISKFYASTARGSIT
                     LPQLLDVPYGAVVFAVVAMALIAFWLIRRFEPSFDATQTYDRAPSPPRLLAGEKVPKA
                     DEGAFAGTASEQDLLHDEHGVPASSASSARPPSSGFATFSPRKKRGGRRRSIREFGAN
                     LTVQFAWAALLLGALAAFAGSPYRAAHATVDIQRLALAVAHEEDHVTAIELAEWIRGR
                     KAGLRIVDLRTPTEFETYHVPRAERIALESLATAPLRATDTIVLISGGGAHAAQAWVL
                     LQALGYKQVYFLRGGLHEWIDDVMNPTILATAPPRAMAEFKRVGEISRYFGGVPRIVD
                     KLEPRPANAAGATEGERSESSTPEAAAAIRRRGC"
     gene            complement(2068..2589)
                     /locus_tag="VNN25_00860"
     CDS             complement(2068..2589)
                     /locus_tag="VNN25_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HXH90100.1"
                     /translation="MTESRPPRPYANPYVAGIGLGLVLLAAFVIMGRGLGASGAFASG
                     AAGVSAAVAPARAAANPFFARYLSGDGPWREWLLFEIVGVILGGFLSALAAGRLRFAV
                     ERGPHIARGTRLGYAFGGGAVMGIGAVLARGCTSGLALTGGALLSAGSWLFMIAAFAG
                     GYLFAPIVKKAWR"
     gene            complement(2586..3512)
                     /locus_tag="VNN25_00865"
     CDS             complement(2586..3512)
                     /locus_tag="VNN25_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH90101.1"
                     /translation="MQLRTLIRVTFVCSILLASSILFASPRQSLVVSADWLKQHISDP
                     DLVLLHVGDKAGYEAGHLPGARLASMADVSVSDHGEKGLMLEMPPAEDLRHKLEALGI
                     SDKSRVIVYYGKDWVSPSTRIIFTLDYAGLGARSSLLDGGQDAWTRAGGTVTKDVPAA
                     KTGTLAPLKIRPIVVDAETVKSRIGAPGYAIVDGRDAVFYDGVETPSHNNEKQRTGHI
                     HGAFSIPYTSITDDRMLLKSPEELAALFAKAGVKPNDTVIGYCHIGQQTTAMLFAART
                     LGHPVLLYDGSFQDWSRHTDYPVDNPSEKAGK"
     gene            complement(3660..4322)
                     /locus_tag="VNN25_00870"
     CDS             complement(3660..4322)
                     /locus_tag="VNN25_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXH90102.1"
                     /translation="MKVLIFGATGSAGGSILRACLASSIVDDVRTITRRPLPLDHPKL
                     HSFVHGDFLDFTPVATAFDDLDALLFCLGVSAAKVSTDEYRVITHDYTMAAANLLKAR
                     SPQAAFHFISGQGTRPGSKIMWSRVKGETENELMDLVGAVCWRPAAIDGEPVGTYRMF
                     QPFYRLLRPFRNLYINGQDFGRAMLQATIEKMRRRVIENREIREIAERASLSADVADE
                     HR"
     gene            complement(4319..5764)
                     /locus_tag="VNN25_00875"
     CDS             complement(4319..5764)
                     /locus_tag="VNN25_00875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH90103.1"
                     /translation="MPFSSFGLHADLLRGVQELGFTRPTPIQNDAIPPAVAGKDVLAC
                     AMTGSGKSAAFLLPILNKLIGKKRGTTRALILTPTRELAAQIDEHLRDLAVHTPLSGA
                     AVYGGVGAGPQEHAFRNGVDVIVATPGRLLDHFQYSYAKLEGLEILVLDEADRMLDMG
                     FLPDIRRVLKHLPPKRQTLFFSATMPAAIVELSREMLKNPVTIAIERKSLPATGITQS
                     VFPVSQDLKMPLLQALLDRGEVENAIIFTRTKHRANRVYEQLERNKVKVARIHGNRSQ
                     MQRTDALAGFKSGRYRILVATDIAARGIDVEALSHVVNFDVPNMPEDYIHRVGRTARA
                     ELTGDAITFVAPDEENDLRAIERAISKRLPRITLPDFDYKQKPAEKLEIPIQERIAAI
                     RAKKAEDRARAKAKLERKSAGSSHAAAPSRQPQRNAGRPAAGSGRAPQRGAAPSHSAG
                     RGPSSAPAHRRPASPSSNRPANSPPGNRRRP"
BASE COUNT         1066 a         1893 c         2009 g         1046 t
ORIGIN      
        1 ggtacgtatg ccgcgagatc gagcagaacg tcgaagccgc gggactttgc cttggcgacg
       61 aacgcgagcg gatgccgcac accggagaag ttcgattgcg ccggaaacgc cagcaatcct
      121 cctctgtccg actcactctc gatgatcgcc tgggcgtcca ggagccgcag ctcatcgtcg
      181 agcgggatgt agcgcacgcg cgctccgcct cggcgcgcgt actcgcggat gccgttcacg
      241 gagttgtgat tgtcggccga caggagaaac gtcgattccg gctgaaaggg atagctctcg
      301 gcgaccagtt tgattgccgc actggtgttg gccgtgaagc agacatcgtg cgtcgactca
      361 tccacgtcga ggaactggag gacgcgccgc cgcgcggtgt cgatgatctc ggtgctggca
      421 tgcgacggcc ccgagtccga gtgcggattc ccaaacaccc cctcggacag cagggcatga
      481 tgcgcgcgga gttgcgactc gccgtagagc gcgctaccgg cgtagtcgag ataggcaaga
      541 tcctgcacgt cgagccggct gaactcgcgc gcgcgcaacg cctcgaacga gatctgcgtt
      601 tcggctcggg cgacgctcag catccacgcc tccggatggc cgcagcggct tccggcgtcg
      661 acgattcgga gcgctcaccc tcggtggcgc ccgcagcatt cgcaggccgc ggttccagct
      721 tgtcgacgat gcgcgggacg ccgccgaagt agcggctgat ctcgcccacc cgcttgaatt
      781 ccgccatcgc gcgcggcggt gcggtggcca ggatcgtcgg attcatcacg tcgtcgatcc
      841 actcatgcag tccgccgcga agaaagtaga cctgcttgta tccgagcgcc tgcagcagca
      901 cccacgcctg cgcggcatgc gcgccaccgc cggagatgag cacgatggtg tcggtcgctc
      961 ggagcggcgc agtggcgagc gattcgagtg cgatgcgttc cgcgcgcggc acatgataag
     1021 tctcgaattc ggtgggagtg cgcagatcga cgatccgcag tcccgccttg cggcctcgaa
     1081 tccactcggc aagctcgatg gcggtgacgt gatcctcttc gtgcgcaaca gccaaagcca
     1141 gacgttggat atcgaccgtt gcatgcgctg cgcgataggg gctgccagca aatgccgcga
     1201 gtgcgccgag gagcaaggcc gcccaggcga attgcacggt gagattcgcg ccaaactctc
     1261 taatcgagcg ccttctcccc ccgcgctttt tgcgggggga gaaggtggcg aagccggatg
     1321 aggggggacg tgccgatgac gctgacgatg ctggaacacc gtgctcgtcg tggagaagat
     1381 cctgttcgct agccgttcct gcaaacgccc cctcatccgc cttcggcacc ttctcccccg
     1441 ctaaaaggcg cgggggagaa ggcgctcgat cataagtctg cgtcgcatcg aatgaaggct
     1501 cgaatcgtcg gatcaaccag aacgcgatca acgccatcgc taccacggcg aagaccaccg
     1561 ctccatacgg aacgtccagc aactgcggca gcgtgatcga accacgtgcc gtgctcgcgt
     1621 agaacttgct gatcgagccg aacgccagcc cggttccgag gacgccggcg aacatgccga
     1681 acatcaccag caatccatcg ccacgtccgg ttgccgccgc gacgcacgat gttcccggac
     1741 agagcccggc tatgacgaac ccgatgccga aaagcagtcc gccggcgagc tgcggcagca
     1801 ggtacgtctc cggcacgtac acccggctga ggtcgagcat gccgaggcgg gcgagccaga
     1861 acgcgccgag catggcggtg acgatcgcgc tgaacatcac cttgaatacg gtgagatcgg
     1921 caaagtagaa ctggccggcg agcttcggtg cgctccccaa ccctgcgcgc tccaacgtcc
     1981 agccgaaggc gatgccgata gcgaccgcgg cgatgagcgc tgctcccggc ccgtggtcgc
     2041 tgagctcgaa gaatggagcg actgcgttca tcgccacgcc ttcttcacga taggcgcgaa
     2101 cagatacccg cccgcgaatg ccgcgatcat gaacagccag cttcccgcgc tgagcaacgc
     2161 gccgcctgtg agcgcgaggc cgctcgtgca tccgcgcgcc agcacggcgc cgatgcccat
     2221 caccgcgccg ccgccgaacg cgtagcccag ccgcgtcccg cgtgcgatgt gcggcccgcg
     2281 ctcgaccgcg aagcgcaacc gcccggcagc aagcgcggag agaaacccgc cgaggatcac
     2341 gcctacgatc tcgaacagca gccactcccg ccacggtccg tctcccgaca gatagcgcgc
     2401 gaagaacgga ttcgcggccg cgcgcgccgg cgcgactgcg gcggatacgc ccgccgcgcc
     2461 gctcgcaaac gcgcccgacg cgccaaggcc gcggcccatg atcacgaacg ccgcgagcag
     2521 cacgagtccg aggccgatgc cggcaacata cggattcgcg tatggtcgcg gtgggcgaga
     2581 ctccgtcact tgcctgcctt ctccgatgga ttgtcgactg ggtaatcggt gtggcgagac
     2641 cagtcctgga acgatccgtc gtacagcagc accggatggc cgagcgtgcg cgcggcgaag
     2701 agcatcgccg tcgtctgctg gccgatgtgg caatagccga tgacggtgtc gttcggcttc
     2761 acgcccgctt tcgcaaacag cgccgccaac tcctcgggcg acttcaagag catccggtcg
     2821 tcggtgatgg aggtgtaggg aatgctgaac gcaccgtgaa tgtgtcccgt gcgttgcttc
     2881 tcattattgt gactcggcgt ttcgacaccg tcgtagaaaa cggcatcgcg tccatcgacg
     2941 atcgcgtatc cgggcgcgcc gatccgcgac ttgaccgtct ccgcgtcgac gacgatcggg
     3001 cgaattttca gcggtgcgag cgttcccgtc ttcgccgccg gaacgtcctt cgtcacggtg
     3061 ccgcccgcgc gcgtccacgc atcctgaccg ccgtcgagca gcgaagagcg cgcgccgagt
     3121 cctgcgtaat cgagcgtgaa gatgatgcgc gtcgacggcg agacccagtc cttgccgtaa
     3181 tagacgatga cgcgcgactt atccgagatc ccgagtgctt ccagcttgtg ccgcagatcc
     3241 tcggcgggcg gcatctccag catcaacccc ttctccccat gatcggagac cgacacatcg
     3301 gccatcgagg cgagccgcgc tccggggagg tggccggctt cgtaaccagc cttgtcgccg
     3361 acgtggagca gaacgagatc gggatcgctg atgtgttgct tcaaccagtc cgcgctgacg
     3421 acgagcgact ggcgcggcga ggcgaagaga atgctgctag cgagcagaat cgagcatacg
     3481 aacgtgacgc ggatgagtgt gcgcaattgc atggatgtct ccttacgagg agcagcatcg
     3541 gtcgcgccgc accaaacgtc accccctttg cagcgagttg gactgaggga ggggtgaagt
     3601 ggcggttggg ggggtcttaa tccgcagatg tcgcagatga acgcagactg gagaccgtct
     3661 tatctgtgtt catctgcgac atctgcggat aggcttgctc gctccgcgat ctcgcgaatc
     3721 tcgcgattct cgatcacgcg gcggcgcatc ttctcgatcg tcgcttgcag cattgcgcgt
     3781 ccgaagtcct ggccgttgat gtagagattg cggaagggcc gcagcagacg gtagaacggc
     3841 tgaaacatcc tgtacgtgcc gaccggctcg ccgtcgatcg cggcgggacg ccagcagacg
     3901 gcgccgacga gatccatcag ctcgttttcg gtttcgcctt tgacgcgcga ccacatgatc
     3961 ttgctgccgg gacgggtgcc ctggccgctg atgaagtgaa acgccgcctg cgggctgcgc
     4021 gccttcagga ggttcgctgc ggccatggtg tagtcgtgcg tgataacccg gtattcgtcg
     4081 gtcgagactt tcgcagccga tacgccgagg cagaaaagga gggcgtcgag gtcatcgaag
     4141 gcggtggcga ccggggtgaa atcgagaaag tcgccgtgta cgaatgaatg aagcttcgga
     4201 tgatcgagtg gcaggggccg ccgcgtgatc gtgcgaacgt catcgacgat cgatgacgca
     4261 aggcaggcgc ggagaatact gccgcctgcc gagccggtcg cgccgaagat caggaccttc
     4321 acggacgccg ccgattgccg ggtggagaat tggccggacg gttactcgac ggcgatgccg
     4381 gccggcgatg ggccggtgcg gatgacggcc cgcgacctgc ggaatgactc ggtgctgcgc
     4441 cgcgctgcgg tgcgcggcct gagccggcag ccggccggcc tgcgttgcgc tgcggctgac
     4501 gcgacggtgc ggcagcgtga ctcgagcctg cactttttct ctccagcttc gccttcgctc
     4561 ttgcccgatc ctccgccttc ttcgcgcgaa tcgcggcgat gcgctcctgg atcgggatct
     4621 ccagcttctc cgccggtttc tgtttgtaat cgaagtcggg gagcgtgatg cggggcaacc
     4681 gcttgctgat ggcgcgctcg atggcgcgaa gatcgttctc ttcgtccggc gccacgaacg
     4741 tgattgcatc gccggtcagc tctgcgcgcg cggtgcggcc gacgcggtga atgtaatcct
     4801 caggcatgtt cggcacgtcg aagttcacca cgtgcgagag cgcctcgacg tcgatgccgc
     4861 gcgcggcgat gtcggtggcc accaggatgc gataacgccc tgacttgaag ccggccagcg
     4921 cgtcggtgcg ctgcatctgg ctgcggttcc cgtggatgcg cgcgaccttg accttgttgc
     4981 gctcgagctg ttcgtagacg cggttggcgc gatgcttcgt ccgcgtgaag atgatggcgt
     5041 tctcgacctc gccgcgatcg agcagcgcct gcagcagcgg catcttcaga tcctgggaga
     5101 cggggaagac cgactgcgtg atgccggtag caggcaacga cttccgctcg atggcgatcg
     5161 tgaccgggtt cttgagcatc tcgcgcgaca gctcgacgat cgccgccggc atcgttgccg
     5221 agaagaacag cgtctggcgt ttcgggggga gatgtttcag cacgcggcgg atatcgggga
     5281 gaaatcccat gtcgagcatc cggtcggcct cgtcgagcac gaggatctcg agcccttcca
     5341 gcttcgcgta cgaatactga aaatgatcga gcagccggcc gggcgtggcc acgatcacgt
     5401 cgacgccgtt gcggaaggcg tgctcctgcg gcccggcgcc gacgccgcca tagactgctg
     5461 cgccggagag gggagtgtgg accgccagat cgcgcagatg ctcatcgatc tgcgccgcca
     5521 gctcgcgggt cggtgtgagg atgagggcgc gcgtcgttcc gcgcttcttg ccgatcagct
     5581 tgttgagaat cggcaggagg aacgccgcgc tcttgccgct gccggtcatg gcgcaggcga
     5641 ggacgtcttt tccggctacc gccggcggaa tggcatcgtt ctgaatcggt gtggggcggg
     5701 tgaatccgag ctcctggact ccgcgcagca gatcggcgtg aagtccgaat gaggaaaatg
     5761 gcacgttgat gactctcttt ctttcgtttg cggcgcgccg gtctggttga cgagcacttc
     5821 cgcgattgaa cgttcggtgg attgatccgg gattgacgtt ggggccaccc gggatcgtat
     5881 gcagggccgg tcgcacgcca gccaatccaa cggaccggga cgatgcgcca tccggtggga
     5941 acgcggcgca gcgctgtttt tattcgatgc ccaaaaggta gtggggggtg gggagtgggg
     6001 agtgggattg cgag
//
LOCUS       DATLVW010000026         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_208221, whole genome shotgun sequence.
ACCESSION   DATLVW010000026 DATLVW010000000
VERSION     DATLVW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208221"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..459
                     /locus_tag="VNN25_00880"
     CDS             118..459
                     /locus_tag="VNN25_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HXH90104.1"
                     /translation="MNIETRKQGDVVIVDFEGRLAVGVGDDLLPRLIEQLLNEGNRKI
                     LLNLSEMDYIDSNGLGELVQSLKVSGRHGASLRLLKPQDRVKKTLKLTNLLPMFSVYD
                     TEADALKAFDA"
     gene            complement(551..1108)
                     /locus_tag="VNN25_00885"
     CDS             complement(551..1108)
                     /locus_tag="VNN25_00885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXH90105.1"
                     /translation="MNRLRVTLSIVFLVITFPTFAEDVISGPLTMAVSTQKKILVVRI
                     GATEVRRYSVAVGTRAKPTPMGQFTVKHIVWNPAWNPPDAKWAKGKAPAGPGDPKNPM
                     KIVKIFFQEPDYYIHGTDAEDSLGSAASHGCIRMAQEDVYKLARYLQDHAGAQKSDDW
                     YAQVINGNQSQSVILPQAVPIAIGK"
     gene            complement(1216..2208)
                     /locus_tag="VNN25_00890"
     CDS             complement(1216..2208)
                     /locus_tag="VNN25_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90106.1"
                     /translation="MRHRNPQDRRCTYLVVIDSDREPLSDLRDFASYLSTLSVAGCEV
                     VVVDGSSTPAFEENGRSFRWVSRHVAARPRQRNFQGSIDPVRAALDFGSCDKVIVAGP
                     NVRYDEDAIGRICALLDTHEVVEPQDYFAPLPWWSGIEEARMLVHRGVDPLPDHGVTF
                     GMRKWPVRGLRSIDVAWSNGVDSVRRLAAQGAEVFSACDVFVRRLPPRLAEWLRERPR
                     QADDDFVLPLKTAFFFTLLPAAVLLAVLGGVRLAGGYAGAVACASMVLAIRGRMGASS
                     VFPLRACLSAPLWVLERSVSVYWALFRKLRGAADDTVGSVLTERKINAKIASGE"
BASE COUNT          476 a          755 c          705 g          440 t
ORIGIN      
        1 actacgtgct cgagatcgac tgcgtcgctc agggggtcac atggttcgcg caagtcggat
       61 cgtcgcccgc cagactcgca ctcgacgtcc ttcccgacga tgtatgatgc gccggcaatg
      121 aacatcgaga cccgcaaaca aggtgatgtc gtcatcgtcg atttcgaagg ccggctggcc
      181 gtcggcgtcg gagacgacct tcttccccgc ctcatcgagc agcttctcaa cgaaggcaac
      241 cgcaagatcc ttctcaacct ctccgagatg gattacatcg acagcaacgg cctcggcgaa
      301 ctcgtgcaaa gcctcaaagt gtccggccgt cacggcgcct cgctccgcct cctcaaaccg
      361 caggaccggg tgaaaaagac ccttaagctg acgaacctgc ttccgatgtt cagcgtctac
      421 gacaccgagg ccgatgcgct caaagcgttc gatgcttagc ctgatttaca gacggcattc
      481 aatatagtaa actctccgca atacaggaga gccatgcccg tcttttcccc ctcccttcgc
      541 gccggctgca tcacttcccg atcgcgatcg gcactgcctg cggcaggatc acactctgac
      601 tctgattgcc gttgatcacc tgcgcgtacc agtcatccga tttctgcgcg ccggcgtgat
      661 cctgcagata gcgcgcgagc ttgtacacat cctcctgcgc catgcggatg catccatgcg
      721 acgccgcgga gccgagtgaa tcttcggcgt ccgtgccgtg gatgtaataa tcaggctcct
      781 gaaagaagat ctttacgatc ttcatcggat tcttcggatc gccgggtcca gccggtgcct
      841 tccctttcgc ccacttggca tccggcgggt tccacgccgg attccagacg atgtgtttca
      901 cagtgaattg tcccatcggc gttggctttg cccgcgtgcc aacggcaacc gagtaccgtc
      961 gcacctcggt cgcgccaatc ctgacgacaa gaatcttctt ctgcgtcgat accgccatcg
     1021 tgaggggacc gctgatgacg tcctcagcaa acgttggaaa cgtaattacg agaaatacga
     1081 ttgagagagt tacgcggagc ctgttcatgc acctgctatt tgcaggtttt gtgtcaacgc
     1141 gagcgaagca gcgtcagcga agccgcgacc gcgagcacga ttgcgaagta gacgaagatc
     1201 gtccagtcga gggccttact cgccgctggc aatctttgcg ttgatcttcc gctcggtgag
     1261 aacggaaccg acggtatcgt cggccgcgcc gcgcagtttg cggaagagag cccaatacac
     1321 gctgaccgac cgttccaaca cccacagcgg tgcagaaagg catgcgcgaa gcgggaacac
     1381 agacgaggct cccattcgcc cgcggattgc cagaaccatc gaggcgcagg cgacggctcc
     1441 cgcatatcca cctgccagcc ggacgccgcc gaggactgcc agcaagaccg ctgccggaag
     1501 gagcgtgaag aagaacgcag tcttgagcgg cagcacgaag tcgtcgtcgg cctgccgagg
     1561 acgttcgcgc agccactccg caagacgcgg aggcagcctt cgcacgaaca catcacacgc
     1621 gctgaacacc tcggcgccct gcgcggcgag ccggcggacg gaatcgacgc cgtttgacca
     1681 ggcgacgtcg atcgatcgca ggccgcgtac gggccacttg cgcatgccga acgtcacgcc
     1741 atgatcgggc agcggatcga caccgcggtg aacgagcatg cgcgcttctt cgataccgct
     1801 ccaccacggc agcggtgcga agtaatcctg cggctcgacg acttcgtgcg tgtcgaggag
     1861 cgcgcagatg cggccgatgg cgtcttcgtc gtagcggaca ttcggtccgg ccacgataac
     1921 tttgtcgcag ctgccaaagt cgagcgctgc gcgaaccggg tcgatcgatc cctggaagtt
     1981 tcgctgcctc gggcgggctg caacgtgccg gctgacccac cgaaagcttc tgccgttttc
     2041 ttcgaacgcc ggagttgaag agccgtcgac gaccacgact tcgcagccgg caacagacag
     2101 tgtggatagg taggaagcaa aatcgcgaag atcgctcagt ggctcccggt cgctgtcgat
     2161 cacgaccagg taggtgcacc ggcgatcctg aggattgcgg tgtctcatgg ccggttggaa
     2221 aagcgagtcg tgtgccatgg ggggcggggt gtggggggtg gggagtggga gatgaatcat
     2281 tttcgggtgt cgggagtcgg gggtcggagc ttcacaccga catccgtgag agtgcctgcg
     2341 actcccctcg aacccgctac cccccccccc cccccc
//
LOCUS       DATLVW010000027         4558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_373355, whole genome shotgun sequence.
ACCESSION   DATLVW010000027 DATLVW010000000
VERSION     DATLVW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373355"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VNN25_00895"
     CDS             <1..346
                     /locus_tag="VNN25_00895"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HXH90107.1"
                     /translation="DQAARSLAEASWIESAPAVSIPAEITAWDLGPGESAVIATCLHL
                     DGARPVLDDLAGRKCARAFRLPVIGTVGLVIAAHRLGHIDARKTLLDLRAAGMWLSDA
                     VIDQALRLAGLN"
     gene            complement(350..1147)
                     /locus_tag="VNN25_00900"
     CDS             complement(350..1147)
                     /locus_tag="VNN25_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXH90108.1"
                     /translation="MRVTVCELPHEPVALAAAWAALCEHTERHASELVLLPELAMIDP
                     LWQDEHFDAARWDAAEARSELWRHRLTELRVAHVVGTRPITIDDRRFNQGFFWSDSGE
                     LLPLRRKFYLPDEPGGWEARWFDRGDSHFTEYHADALSFGLNICTELWAVETYAAYAA
                     RGVQVILSPRATAAATTNKWLSVGVVAAVRSGAFSLSSNRVDPTGACGGVGWIIDPSG
                     HILALTTPDAPFATVDIDLSAAAEAKSEYPRYVFGGRRGERGSSGSV"
     gene            complement(1157..3217)
                     /locus_tag="VNN25_00905"
     CDS             complement(1157..3217)
                     /locus_tag="VNN25_00905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXH90109.1"
                     /translation="MHIDAFHNTGSRNNGHLLVIALPVVMAAALAWWYASPSAAQPAP
                     DQKPRLESTVDASIKPGDDFFAYANGAWLKATAIPAGKQRWGTRDELEGLARRRIAEL
                     LDTASAAPAGSPARKVADFRAAYMNEAAIEARGLAPPQPLLNQIEKVSDKTQLTRLLG
                     RGMRADVDPEGFGIYDSASVLGLSVEQSIHGEKTNVAFLLQGGLGLPNREDYLSADSA
                     KRERHRQSIAKMLRLAGFDRADERASAVLALETAIAQSHSTSEASSNDHNADNVWTRA
                     DFAQRAPGMDWTAFFDAADLGRQQELVVWQPTAVTGLAALVASQPLETWKDYLRFHAI
                     HDYADVLPRAFADEAGAAPSRAERALAATQSAMSDAIGRMYAERYFPAAQKARLERIS
                     DNVRAALIKRVEAATWMTAATKAGSLEKLKTLYVGLGYPDQWEDYGSLTVDPKNPLGN
                     LQRVSDRAYRNALARLGQPVNLKYWYSAPQTVAALLVFQQNSYVMDAALLEPPKYDHA
                     SSDAAAYGSVGALIGHDLTHYIDTLGADYDTEHRMRHWWTAEDMQHFEALAQPLVDQF
                     AAYQPLPGLSINGKLTLRENIADLGGLAAALDAFHKTLGSRLTDTDYVRKLDREFFIA
                     YAQTQRRKISEDANRKQIATNDHAPEDYRADTVRNLDAWYDAFDVRPGQRLYLEPAAR
                     VRVW"
     gene            3426..4481
                     /locus_tag="VNN25_00910"
     CDS             3426..4481
                     /locus_tag="VNN25_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH90110.1"
                     /translation="MNTRAQLIDVLFVIVPHSLLLDIAGPAEAFRLANQHRELRGLPP
                     RFRLSFAGPVTTMPTSVGLALGDLVPLPEQLHAPTWVVVVGQPTVHVTNVTPEIAVTA
                     QWLRDRLREALVRADTPHRLVTICSGTLLAARAGLLGTRRCTTHHELLPMLRALAPRA
                     KVIENRVFVVDGPLASSAGITAGIDVALHLIAEECGEALAASVAEDMVVYLRRSPRDT
                     ELSPFHMHRRHLHPTVHRVQDAIVSEPQRDWDMASLADVGHAAERHLLRLFVDHAGIS
                     PLHYLRLIRLERARQSLEYGATVTRAAAVAGFGSDVQLRRAWSRQWGGSPRDAARAAE
                     SAASAPRSPRTTRSRSR"
BASE COUNT          731 a         1473 c         1529 g          825 t
ORIGIN      
        1 agatcaggcg gcacgctcgc tcgccgaggc aagttggatc gaaagcgctc cagcggtctc
       61 aattccggcc gagatcactg cgtgggatct cggacccggc gagtcggcag tcatcgcgac
      121 gtgtctgcac cttgatggcg cgaggcccgt gcttgatgat ctcgccggca ggaagtgtgc
      181 gcgcgcgttc agattgcccg tcatcggaac cgtcggtctg gtgatcgcgg cccatcgcct
      241 cgggcacatc gacgcacgga agactctgct cgacctacgc gcggcaggaa tgtggctatc
      301 ggatgctgtc atcgatcagg ccctgcggct cgctgggctg aactgagaat cagaccgatc
      361 cgctcgaccc gcgctcaccg cgtcgccccc cgaacacgta acgcggatac tcgctcttcg
      421 cctccgccgc ggcggacaga tcgatgtcga ccgtcgcgaa tggcgcgtcc ggcgtcgtga
      481 gcgccaggat gtgaccggac ggatcgatga tccatccgac gccgccgcat gcgccggtcg
      541 gatcgacgcg gttggacgac aagctgaatg cgccggagcg cacggccgcc actacgccaa
      601 ccgacagcca tttgttcgtc gtggcggcag ctgtcgcgcg gggcgagagg atgacttgca
      661 cgccgcgggc ggcgtaggcg gcgtaggtct cgacggccca cagttcggtg cagatgttga
      721 ggccgaacga aagagcgtcg gcgtggtatt cggtgaaatg ggaatcaccg cggtcgaacc
      781 agcgcgcttc ccagccgccc ggttcatccg ggaggtagaa cttgcgtcgc aacggcagca
      841 gttcgccgga gtctgaccag aagaaaccct ggttgaagcg acggtcgtcg atggtgatcg
      901 gtcgggttcc gacgacatgc gcgacgcgca actcggtgag gcgatggcgc cacagctcgc
      961 tgcgcgcttc tgcggcatcc cagcgggcag cgtcaaagtg ctcgtcctgc cagagcggat
     1021 cgatcatcgc cagctccggc agcagcacca gctcggaagc gtggcgctct gtgtgctcgc
     1081 acaatgcggc ccatgcggcg gccagcgcta ctggctcgtg cggcagttca cagaccgtga
     1141 cacgcatggt cgcttgctac cagacccgca cgcgcgccgc gggctcgagg tagagccgct
     1201 ggcccgggcg cacgtcgaag gcgtcgtacc acgcatcgag attgcgaacc gtgtcagctc
     1261 ggtagtcttc cggcgcgtgg tcgttggtgg ctatctgctt tctgttggcg tcctcgctga
     1321 tcttgcgccg ttgcgtctgc gcgtaggcga taaagaactc gcgatccagt ttgcgcacgt
     1381 agtcggtatc cgtcagtcga ctgccgagag tcttgtggaa ggcgtcgagc gcggcggcga
     1441 ggccgccgag gtcggcgatg ttttcgcgga gcgtgagttt gccgttgatc gagaggccgg
     1501 ggagcggctg gtacgccgcg aactgatcca cgagcggttg cgcgagagcc tcgaagtgct
     1561 gcatatcctc ggcagtccac cagtggcgca tccgatgctc cgtgtcgtag tcggcgccga
     1621 gggtgtcgat gtagtgcgtg aggtcgtgtc cgatgagcgc cccgacggaa ccgtaggcag
     1681 cggcgtcgga cgacgcgtga tcgtacttgg gtggctccag caacgcggca tccatcacgt
     1741 acgagttctg ctgaaacacg aggagcgcgg cgaccgtctg cggcgcgctg taccagtact
     1801 tcagattgac gggctgtccg aggcgggcga gggcgttgcg gtaggcgcgg tcggagacgc
     1861 gttgcaggtt gcccagcgga tttttcgggt caacagtcag cgacccgtaa tcctcccatt
     1921 gatcgggata gccgaggccg acgtacaacg tcttcaactt ctccagcgaa ccggccttgg
     1981 tggccgccgt catccacgtc gcggcctcga cgcgcttgat gagcgccgcg cggacgttgt
     2041 cggagattcg ctccaggcgc gccttctgtg cggcggggaa gtagcgctcg gcgtacatcc
     2101 ggccgatcgc gtcgctcatt gccgattgcg tggctgcgag ggcgcgttcg gcacgtgacg
     2161 gagccgcgcc cgcctcgtca gcgaatgcgc gcggcagcac gtcggcgtaa tcgtggatgg
     2221 cgtggaagcg caggtaatcc ttccatgtct ccagcggctg cgatgcgacg agcgccgcca
     2281 gaccagtgac cgcggtcggt tgccagacta cgagttcctg ctggcggccc agatctgccg
     2341 catcgaagaa cgcggtccag tccatgccgg gtgcacgctg cgcgaagtcg gcgcgcgtcc
     2401 agacgttgtc ggcgttgtgg tcgtttgacg atgcctcgga cgtgctgtgg ctttgcgcga
     2461 tcgccgtttc cagcgccagc accgcgctcg cgcgttcgtc ggcgcggtcg aagccggcga
     2521 gccttagcat cttcgcgatc gattggcgat ggcgttcccg ctttgcggaa tccgcgctga
     2581 gataatcctc gcgattgggc agtccaagtc ctccctgcag caggaaggcg acgttggtct
     2641 tttctccgtg gatgctttgc tcgacggaga ggccaagcac gctggcggaa tcgtagatgc
     2701 cgaatccctc gggatcgacg tcggcgcgca tcccgcggcc gaggaggcga gtgagttgcg
     2761 ttttgtccga aaccttctct atttgattaa gcagcggctg gggcggcgca agacctctgg
     2821 cctcgatcgc agcttcattc atatatgcag cgcgaaagtc cgcaaccttg cgcgcgggcg
     2881 agccggccgg cgcggcgctg gcggtatcga gcagttccgc gatgcggcgg cgggccaaac
     2941 cttccagctc atcgcgcgtt ccccatcgtt gtttgcccgc cgggatagcg gtggccttga
     3001 gccaggcgcc gttggcatac gcgaagaaat cgtcgcccgg tttgatgctc gcgtccacgg
     3061 tgctctcgag ccgcggcttc tggtcgggtg ccggttgcgc agcggacggg gacgcatacc
     3121 accacgccag cgccgccgcc atgacgaccg gcagcgcgat cacgagaagg tgtccattgt
     3181 tccggctacc agtgttgtgg aatgcgtcga tgtgcatgcc gcgaaggtac cgacgctgcg
     3241 atgtcctgtc gcgacgcagg acgacaacct tcaatcactt tcggacatgc acctgaaaga
     3301 aaaaaccacc acagagaccc agagagcaca gagaacttct ccgtgacctc tgtgtctctg
     3361 tggtaaatct cgtgggtcgg gtgagtggcg tggcatagac atatgctttg attcgctagg
     3421 agcacatgaa cacccgcgca cagctcatcg acgttctctt cgtcatcgtg ccgcattcgt
     3481 tgctgctcga catcgccggg cccgcggaag catttcgtct cgccaaccag catcgcgaac
     3541 tgcgcggact gccgccgcgc tttcgtctca gcttcgccgg gccggtgacg acgatgccga
     3601 cgtcggtcgg actggcgctt ggggacctcg ttccattgcc ggagcagctc cacgcgccga
     3661 cctgggtagt ggtcgtcggc caaccgaccg ttcacgtgac gaacgtgacg ccggagatcg
     3721 cggttaccgc gcaatggttg cgcgatcggc tgcgcgaagc gctcgtccgc gcggacacac
     3781 ctcaccgcct tgtgacgatc tgctcaggca ccctcctcgc cgcgcgagcg ggcctcctcg
     3841 gcacgcgccg ttgcacgacg caccacgagc tgttgcccat gcttcgagcg ctcgcgccgc
     3901 gcgcaaaggt catcgagaac cgcgtcttcg tcgtggacgg tccgctcgcg tcgagcgcgg
     3961 gcatcacggc ggggatcgat gttgcgctcc atctgatcgc cgaggagtgc ggggaggcgc
     4021 tggcggcaag cgtggccgag gatatggtgg tgtacctgcg gcgctcgccg cgcgacaccg
     4081 agctgtcgcc gttccacatg caccgccgcc acctccaccc gaccgtgcac cgcgtgcagg
     4141 acgcgatcgt ctccgagccg cagcgcgact gggacatggc atcgctggcc gacgttggcc
     4201 acgcggccga gcgacacctc ttgcgtctgt tcgtcgacca cgccggcatc tcgccgctcc
     4261 actacctccg cctgatccgg ctggagcgcg caagacagtc cctcgaatac ggcgcgaccg
     4321 tcacgcgggc cgccgcagtc gccggcttcg gctccgacgt gcaactgcgc cgcgcctgga
     4381 gccgccagtg gggcggctcg ccgcgtgatg ccgcgcgcgc ggcggaatcg gccgcctcgg
     4441 cacctcgatc accgcgcacg actcgatctc gcagccgctg agacgctcgc ttcggtcggt
     4501 gtacgatgcc tccacaccat cggaggcgcg gatttcacca gccagtttgc atagtgtc
//
LOCUS       DATLVW010000028         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_43337, whole genome shotgun sequence.
ACCESSION   DATLVW010000028 DATLVW010000000
VERSION     DATLVW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43337"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..493)
                     /locus_tag="VNN25_00915"
     CDS             complement(152..493)
                     /locus_tag="VNN25_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXH90111.1"
                     /translation="MHYPFEKHFLVCVGGRCSDPERGIERGEFIHAELKGHNKKLGRK
                     ATVRVCTVSCLELCDHGPNMIVDDIVYSHLTRETAREVYAGVMGDGPPRDDLQLSEVE
                     FRAGGSAALKK"
     gene            complement(612..2012)
                     /locus_tag="VNN25_00920"
     CDS             complement(612..2012)
                     /locus_tag="VNN25_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HXH90112.1"
                     /translation="MIKFGTSGWRGIIGEDFTFSNVRIASQGITNYLKKSNLKGNGVV
                     IAYDTRFLSEKFALEAARILAFNGIQAYLCTRDVPTPTAAFETVRRKAMGAINFTASH
                     NPPEYNGLKFSTSNGAPALPEITKQIEEEIRIVAETGERIDVYEKAELIEEIDPKDRY
                     LNELKQKVDVDVIRKSGLRIAIDSLYGTSRDYLDYFLLESGVEVKIIHNYRDPYFGGF
                     SPECNEKNLGELRKVVTDEGFDLGLATDGDADRFGIIDDRNRFVSPNVIISLLALYLK
                     RDRKIPGGLARSVATTHLIDAIARKLDVELFETPVGFKYIGELILEDKIAMGGEESAG
                     MSMYRHLPEKDGILACLLVAEMVAATGKKLNQLVEEMYAEFGYFYSKRIDMKLTPQLK
                     ESLAEKLANPPKSIDAFTVRSVNKTDGVKLIFDDNNWLLFRLSGTEPVARIYAEACSP
                     RDLKNVLDAGKKWALS"
BASE COUNT          342 a          597 c          666 g          434 t
ORIGIN      
        1 ccgctgtcgc gtttgaattc gacgatcagc atcggcagca cgcccgatcc ctgcaccgtc
       61 gtggtcgtcg ccaggacgaa cgatttcgct ttcggctccc gcgcgcatgc ggccagcagc
      121 agcgcgattg cggcaagtgc acgacgcatc gctatttctt caacgccgcg ctgccgccgg
      181 cgcggaactc cacttcgctc agttgcaggt catcccgcgg cggcccgtcg cccatgacac
      241 ccgcgtagac ctcgcgcgct gtctcgcgcg tgaggtgcga gtagacgatg tcgtcgacga
      301 tcatgttcgg tccgtgatcg cacagctcga ggcacgagac cgtgcagacg cgcaccgtgg
      361 ccttgcgccc gagtttcttg ttgtgcccct tgagctcagc atggatgaat tcgccccgtt
      421 caatcccgcg ttcgggatcg ctgcatcggc caccgacgca gacaaggaaa tgcttttcga
      481 aggggtagtg catggttagg gtgtggggag tagggggtgg ggggtgggag aggactgttg
      541 gggtgtcgag ggagcgggcc ttgagctcca ttctttctcc caccccccac cccctactcc
      601 ccacacccca ctcacgacaa cgcccacttc ttccccgcat cgagcacgtt cttcagatct
      661 cgcggactac acgcctcggc gtaaatgcgc gcgaccggct cggtgccgga gaggcggaag
      721 aggagccagt tgttgtcgtc gaagatcagc ttcacgccgt cggttttgtt gacggagcga
      781 acggtgaagg cgtcgatcga cttcggcggg ttggcgagct tctcggccag cgattccttg
      841 agctgcggcg tcagcttcat gtcgatgcgt ttggagtaga agtagccgaa ctccgcgtac
      901 atctcttcga cgagctgatt cagtttcttg ccggtggctg caaccatctc cgccacgagc
      961 aggcaagcca ggatgccgtc cttttccggc aggtggcggt acatcgacat gcccgcgctc
     1021 tcttcgccgc ccatggcgat cttgtcctcg aggatcagct cgccgatgta cttgaagccg
     1081 accggggtct cgaagagctc gacgtcgagc ttgcgggcaa tcgcgtcgat gagatgcgtc
     1141 gtggccacgg agcgcgctag gccgccgggg atcttgcggt cgcgtttcag gtagagcgcg
     1201 agcagcgaga tgatcacgtt cggcgagacg aagcggttgc ggtcgtcgat gatgccgaaa
     1261 cggtcggcgt cgccgtcggt ggccaggccg aggtcgaagc cctcgtcggt gacgaccttg
     1321 cgcagttcgc cgaggttctt ctcgttgcat tcgggggaga agccgccgaa gtaaggatct
     1381 cgatagttgt gaatgatctt gacctcgaca cccgactcga ggaggaagta gtcgaggtaa
     1441 tcgcgcgagg ttccgtaaag cgagtcgatg gcgatgcgca aaccgctctt gcggatgacg
     1501 tcgacgtcga ctttctgttt gagctcgttg aggtagcggt ctttcggatc gatctcttcg
     1561 atcagctcgg ccttctcata gacgtcgatc cgctctccgg tctcagcgac gatgcggatc
     1621 tcttcctcga tctgcttcgt gatctcgggc agcgcgggcg cgccgttgga ggtggagaac
     1681 ttcagtccgt tgtactcggg gggattgtgc gaggcggtga agttgatcgc gcccatcgcc
     1741 ttgcgccgga cggtctcaaa cgcggcggtg ggggtgggga cgtcgcgcgt gcagaggtag
     1801 gcttggattc cgttgaaggc gaggatgcgg gccgcttcca gcgcgaactt ctcggagagg
     1861 aaccgcgtgt cgtaggcgat gacgacgccg ttccctttca gattcgactt cttcaggtag
     1921 ttggtgatgc cctggctggc gatgcggacg ttgctgaaag tgaaatcctc gccgatgatg
     1981 ccacgccatc ccgaggtccc gaatttgatc atggttctcc aatcaatgcg gcgtgggaa
//
LOCUS       DATLVW010000029        14554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_249220, whole genome shotgun sequence.
ACCESSION   DATLVW010000029 DATLVW010000000
VERSION     DATLVW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249220"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VNN25_00925"
     CDS             <1..353
                     /locus_tag="VNN25_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH90113.1"
                     /translation="PHPPLPTPGVGNDIPLGATGVTGLDADFPYTIYIDRMKSLIGQH
                     YLRPGAARAGTIGTVFFTVDRDGKIRDARSEISSGDSTFDRAALRAVLEASPLPPLPF
                     GYNGTYLGVHLTFR"
     gene            350..1612
                     /locus_tag="VNN25_00930"
     CDS             350..1612
                     /locus_tag="VNN25_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90114.1"
                     /translation="MKPKTLLLSLTLLLAALPLAAQTQIQTTLTPGQVITIARIAVPR
                     PETTLPLETINTPFFAPLTRDLAYSGVFAIAPIPPGNLNSIELIKAAGAQFLLALKID
                     KQGDEYVVDARLIDSTGAQQFGQRYRGGAGALTRIAHTLTNDLVRVVNGKPGIFMSMI
                     GFASNRSGNFEIWLMDWDGANQRQITHHNALSILPSWSADSQRMVYTSFFRGTSDMYI
                     ISRRGGARVHVPTGLNLNTSATFSPISNDIAFVGSVAGNPDIYVVKDDGTNLRRLTHD
                     SSIESTPEWSPNGTQISFTSGRSGSPQIYTMDAEGSNVQRISFEGEWNDDATWSPDGS
                     QLAYTSRVNGRFQIRVADLATKTSRILAGEGSNEQPTWSPDGKWIAFQSNRSGKWQIY
                     RMRVDGGTDLLQLTFDGENKDPDWSKKE"
     gene            1799..2401
                     /gene="pal"
                     /locus_tag="VNN25_00935"
     CDS             1799..2401
                     /gene="pal"
                     /locus_tag="VNN25_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXH90115.1"
                     /translation="MNKAFKAFAVTIALLALIAAPACKKKVAPVVAPSVDTSMTVPPV
                     PATVTTATAQTVTETPDFVSPKTDTIAVDTLPGDIEELNRVAQSRGYVQDAFFEFNDS
                     TLTAAAQTALTSSATWLKKNGQYNLLIEGHCDERGTEQYNLALGDRRANQAKEYLVTL
                     GVDAGRIRTVSYGEERPFDPGHDESAWAKNRRDHLVLVGK"
     gene            2416..3264
                     /gene="ybgF"
                     /locus_tag="VNN25_00940"
     CDS             2416..3264
                     /gene="ybgF"
                     /locus_tag="VNN25_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXH90116.1"
                     /translation="MQSFIRIALRITLLGAATLSSVACVTSSDVEKLQSQISDLQEQL
                     VQVKRTSSSKEEVQNVNKTIADQTQMLLKSNATLVAKVDQIEDKMQGGQGAVEQTNNR
                     LDRIAQQLTQTQHDVDALKAAIAQASAAATANVTVPATSGSELTVQPPAGTAEDPMQV
                     YQSAYRDYQRGNYDLAIAGFRDFASRNPNSELSGNAAYWIGESLYSQKKYREAIQQFD
                     SVVTRYPKSDKVAGALLKKGYGYIALGEKSQGIVQLQYVVHEHPTSPEAALARQKLKS
                     LGVDSK"
     gene            3310..3570
                     /gene="rpsT"
                     /locus_tag="VNN25_00945"
     CDS             3310..3570
                     /gene="rpsT"
                     /locus_tag="VNN25_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXH90117.1"
                     /translation="MANTRSAEKQQRQAEKANTRNRAGKSRLRSALKKTRAAVDAGGA
                     DASVLSTGFSEIDKAAKRGVIKGNTADRYKSRLAKASKRAEK"
     gene            3680..3829
                     /locus_tag="VNN25_00950"
     CDS             3680..3829
                     /locus_tag="VNN25_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90118.1"
                     /translation="MSARLSSRDRIEHASLTTIELVIFWGVIGAVLYLIAERGRGQAA
                     GVSLS"
     gene            complement(3850..4788)
                     /locus_tag="VNN25_00955"
     CDS             complement(3850..4788)
                     /locus_tag="VNN25_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90119.1"
                     /translation="MSDSTFNVSKDSIQISYRRFLSESAAGFVLILLFAASYYYPVFT
                     RYSLRASFGGDPHIGSETKVFVFVTLFLLATPLGYAVNAFSWLLFSQVQTALETFFCR
                     PGTHRIFPILSNFEARHVSPIMKELGIDDATFATMNWFLRTSLENPHLEKLIAETSVR
                     NLVIFLRNTALFLVSGGLIALYSAQRTGLAKAWCAAIMLLIAFALTTTWTYKPIARIL
                     AIGGVFGVGALVYVASDLELAARASFLIVTSVVVVLIAGVIGYYNCCAILLHTHLARA
                     AVGVGTAASSDNVNKPFGIITALVTEAAKLERERKI"
     gene            complement(4961..5179)
                     /locus_tag="VNN25_00960"
     CDS             complement(4961..5179)
                     /locus_tag="VNN25_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH90120.1"
                     /translation="MSRVPIDHILELPTAERVAMVQEIWESMLEHPENVEITAAQRDE
                     LERCWVELQQNPDDGESWSDLKRSLMNE"
     gene            complement(5198..6403)
                     /locus_tag="VNN25_00965"
     CDS             complement(5198..6403)
                     /locus_tag="VNN25_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH90121.1"
                     /translation="MTDHLLQYRDRFPILNKTKYLVSHSLGAMPEATREALVEYADLW
                     ASRGVRAWGDKWWMMSIEVGDIIAPLIGAPPGSVVMLPNVTTAEAVVLSSYEYTAPRN
                     RVVIVDGEFPSVRYIYDRLARRLGAEIVNVPHDSSGLGFDLDRLLAAIDERTQLVPIG
                     QILFESSYMIDVEAIARRCREVGATLVLDVFQSAGIVPVDVTGWDVPIAVGGVLKWLC
                     GGPGGSFLYVDPALRPKLEPSFTGWMAHANPFAFEAPPMRFRDDALRFALGTPPIPAL
                     YAAREGPKVIAEASGGDMNVIRAKSLRQTQRIMDLADARGFELRTPRESDRRGGSVSV
                     LMPHAKEVAYELNAEDIVCDFRPGAGVRFSPHFYTTDEELEIAFATVDEILRTDRWKR
                     QAAKQTIVT"
     gene            complement(6400..7527)
                     /locus_tag="VNN25_00970"
     CDS             complement(6400..7527)
                     /locus_tag="VNN25_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HXH90122.1"
                     /translation="MTSKEEGVRATNQDFIRYDQVEKHQTYQLAEGLDENQFDDDFQV
                     RTVDVSRFLHGNADDKRAFATQLGDALHEIGFAIIEGHGVDPSLYAEAERRIEEIFST
                     LTLEEKMRFRAERFGSVNQGYFPIKETSNMHPDLVEGWVFCRRAFEDPAKFWPVVGSG
                     LKSKVPAQDHFSSDDVRGTLDFRPDPAFFEQIVHAHERLILPIMQSLLTYLGCDAHLF
                     DAKLTGTNFGLRLNYYPPVSASDDASGAARLLGHEDIDMFTFLPAPRVEGLQVLNRRN
                     MKWVRIAAPPGSIILNTGDYMQRISNDIFPSTTHRVSKPRDPEQRNKTRVSFPMAVYL
                     WEDELLEVLPCIANPKYPPVKAINFHTSITSKFYGDDYAVK"
     gene            7550..8305
                     /locus_tag="VNN25_00975"
     CDS             7550..8305
                     /locus_tag="VNN25_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH90123.1"
                     /translation="MVAKLDGGCLVAGLKEGEPVVDGALRIWKHFGREAGAKAISLRV
                     LELPAGERATLRNEVADEVLYVIAGSGAAGDSAISVDSGIYLPPSASLDIVAGETLTL
                     VSSQCPDVDGGQIAAGPAAGQPAGRRRYGGALVVAMRDRPVERTGDRWYREIINAGDG
                     SIQVTQFVGGIPPGRSPDHYHLYEEVIVILEGTGVMWAGTSNTPIETGSCIFLPHGQV
                     HCVENRGEGEMRLLGMFYPAGSPAVRYGANGKP"
     gene            8330..10633
                     /locus_tag="VNN25_00980"
     CDS             8330..10633
                     /locus_tag="VNN25_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90124.1"
                     /translation="MRWTVLALLVLSSSTLAADTHVTLTGASEGAEVCRFQARDREKP
                     IERWLSAQAVTCVATDAALTFPPGLWNVFARAKGMVSVEPMLVDGNSAPANLALSLVP
                     AATVVVQLPAEARGVLYAPKHAIAFPASERTTVPAGEELWLIVIVKAAPVAIVPIASL
                     EPGIERVVDARAINDAPAVLGWIHVSEADRAAIKTARGVQLPHIGITATGKEVVAASL
                     PVQDALNGAFVLFRGLSAGSADLRLDGRGWLPFRRSLRVEPKPVTLLREPIAARASTT
                     IIVNWSTSGDLAALDRSLGSCETPKEAPSLELTIAACPEPKPGKSLDPATCSVIRKET
                     LRNDLSYGSVRVDEVPPGMYRAELRFGRLPPFDVMEDVPPLQQRPMAPLQAKYFEAYG
                     SLTRGGEPLHEDARILFPHDGVGFAPRDTGEYRGVVKEGFRSDAKIDVVTCSGKRAYV
                     ITDREMNVWARTRFDIDIPDNTLTVTVVDTFTQRPLPPATLKYVVMSLQGRFPLLTRE
                     VSQSDEGDERGKQVAGQFVIKGLPERKLLLTVSCPGYKKKELDPFSMTKSEKKEIDVD
                     LVPLGGSEARVISSRPFANGIIFWFSSTGAEMERAELAPDGTFHFEQTHYRDETMTVV
                     SASHPLWILRAPPVEKATPLQVKFPDAAPQRDVEVTIYNMPPRLLTPIGVAIGGLRVP
                     QPALAQHLALRGTAPLVKGGGPLLIPGLAESGPIDILRGPPGAQRLQQSLPLPPLMDP
                     SLRNFAPVATQRLEPGSGAVAFDGAAK"
     gene            complement(10714..11118)
                     /locus_tag="VNN25_00985"
     CDS             complement(10714..11118)
                     /locus_tag="VNN25_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90125.1"
                     /translation="MLNVIAAFVLTFTAIDLHDPAVRGVLTELLRNARYGCVNTEEAA
                     FLIRDAHGATFFLRWRANGELNRASWRGPIPAGTVAIVHTHPNWLPLPSNIDARVARE
                     TSLPVYVVTLTRIARTDGGKPVLVVRGDWSGS"
     gene            complement(11178..11537)
                     /locus_tag="VNN25_00990"
     CDS             complement(11178..11537)
                     /locus_tag="VNN25_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90126.1"
                     /translation="MAPDKNDKIPTSSIVMKAIDAKTFSELEAFVKDLEARPTDRLLR
                     DLPDLVALSESKASIIAYVLATKFRHADEDGRHTIRESVAGTLERLPPGEERERVSHV
                     LDRLHGLQNAPRVTDFE"
     gene            complement(11695..12225)
                     /locus_tag="VNN25_00995"
     CDS             complement(11695..12225)
                     /locus_tag="VNN25_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH90127.1"
                     /translation="MATKKTGKTKKPSAKAKSAKRVAKPAAKKAAKKSAAKTASAKKD
                     SGGLRLLSASPSFTVNDINKSLAWYRDVLGFTVGDRWEMDGVLRGVELSAGDVLFMIG
                     QDDWQKGRDRVKGVGVRLYCETDQDIDKIAARIKAAGGTLAQEPTDQSWGYREISLDD
                     PDGYKLTIGKALKKKR"
     gene            12586..13020
                     /locus_tag="VNN25_01000"
     CDS             12586..13020
                     /locus_tag="VNN25_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HXH90128.1"
                     /translation="MARLLDVNVLVALFIPEHEHHAAAMKWFSNEAAIAGWATCVITE
                     LGAIRVCAQLPGGLWPPEATADRLLMLTISNRGYVWWPETVSPAVVPEVRSAGTAKHV
                     TDRYLLGLARQNGGQVVTFDRALAVAGGDDVIGLLPVGGEVP"
     gene            complement(13118..13363)
                     /locus_tag="VNN25_01005"
     CDS             complement(13118..13363)
                     /locus_tag="VNN25_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH90129.1"
                     /translation="MQRRMLKTIVSSKGRIVLPAALRKQDGIVPGDEFIVERLDRGQY
                     RLVRRARKPNEGVLDWLLACPEKGFFVPIETESTDTL"
     gene            complement(13382..14428)
                     /locus_tag="VNN25_01010"
     CDS             complement(13382..14428)
                     /locus_tag="VNN25_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90130.1"
                     /translation="MNITNLRIAAWRVALCGAFLFAAQTLYAADPTYTVSMQALPGAS
                     DAGIGMDYIIFDPATRSVWVPAGNTGAVDVVDPATGKVRQITGFPTAEMGTGDRKRVV
                     GPSSVTIGDGTIYIGNRGDSTVCAVNPKTLVRGTCHKVDSMPDGLAYVAGMKEVWVTT
                     PRDKSIRILDAATLNEKAKLTFEGNPEGFAVDGKRGRFYTNMEDKDVTLAIDLGTHKT
                     VATWKSTCGADGPHGLRVDEKAGHLFIACSARTEVMDVGHDGAVLSSIDTGDGADDLD
                     YASATHMVYIGAAKAGLLTVAHADAKWILSIVAKVPVHTGARNPAVTSDGTVYLAHSG
                     LGKLSDLVVATPKK"
BASE COUNT         2817 a         4412 c         4510 g         2815 t
ORIGIN      
        1 ccccccaccc cccactcccc acacccggcg taggcaatga catcccgctc ggcgccaccg
       61 gcgtgaccgg actcgacgcc gatttcccct acactattta catcgaccgg atgaaatccc
      121 ttatcggcca gcactatctg cggcctggcg ccgcccgggc gggaacgatc gggaccgtct
      181 tcttcaccgt tgatcgcgat ggcaagatcc gcgatgcaag aagcgaaatc tccagcggcg
      241 acagcacgtt cgaccgcgca gccctccgtg cagttctcga agcttctccg cttccgcccc
      301 ttccgttcgg ctacaacggc acgtaccttg gagtgcattt gaccttccga tgaaaccgaa
      361 aactcttttg ctctcgctga cccttctgct ggcagccctt ccgctggcag cacagacaca
      421 gatccagacg acgctaaccc ctggtcaggt catcacgatc gcgcgcatcg ctgttccccg
      481 cccggaaacg acgctgccgc tggagacgat caacacgccg ttttttgcgc cgctgacgcg
      541 cgatctcgcc tattccggcg tcttcgccat cgcgccgatt ccgccgggaa acctcaatag
      601 catcgagctc atcaaagccg cgggcgcgca attcctcctg gcactcaaaa tcgataagca
      661 gggcgacgaa tacgtcgtcg acgcccggct catcgactcg accggggcgc aacagtttgg
      721 gcaacggtat cgcggcggcg ccggcgcgct tactcgcatc gcccatacgc tgaccaacga
      781 tctcgtccgc gtcgtcaacg gcaagccggg catcttcatg tcgatgatcg gcttcgcctc
      841 gaaccgctcc ggcaacttcg agatctggtt gatggattgg gacggcgcga accaacgcca
      901 gatcacgcac cacaacgcgc tctcgattct cccgagctgg tccgccgaca gccagcgtat
      961 ggtctacacc tcgttcttcc gcggcacgag cgacatgtat atcatcagcc gccgcggcgg
     1021 cgcgcgcgtc cacgtgccca cggggctcaa tctcaacacc tctgccacct tctcgccgat
     1081 cagcaacgac atcgctttcg tcggctccgt cgccggcaat cccgacatct acgtcgtgaa
     1141 agatgacggc accaacctgc gccggctgac gcatgactcc tcgatcgagt cgacgccgga
     1201 gtggagtccg aacggaaccc agatctcctt tacctcgggg cgcagcggtt cgccgcagat
     1261 ctatacgatg gatgccgagg ggtcgaacgt gcagcggata tcgttcgagg gcgagtggaa
     1321 tgatgatgcc acctggtcgc cggacggatc gcagctcgcg tacacatcgc gcgtcaacgg
     1381 acgcttccag atccgcgtcg ccgatctggc aacgaaaacg agccgtatcc tggcgggcga
     1441 aggctccaat gaacaaccga cctggtcgcc ggacggcaag tggatcgcct tccaatcgaa
     1501 tcgaagcggc aaatggcaga tctaccggat gcgcgtcgac ggcggcacgg acctgctgca
     1561 actcacgttc gacggagaaa acaaagaccc tgattggtcg aagaaagagt gaaagcagag
     1621 atcagagggt gagacagaga tcagagacta gagatcagag acaaggggct tcctgatctc
     1681 tgatccctga tttctgatac gtctgatatg cctgatctct tgtctctgct tgtctctgct
     1741 ttctgatctc tggtctcttg tctctgctgt actagaatcc gcaaagaggt aactaacaat
     1801 gaacaaagca ttcaaagcct tcgcagtaac catcgcactt ctcgcactca tcgccgcgcc
     1861 ggcctgcaaa aagaaagtgg cgccggtcgt ggccccctca gtcgatacct cgatgaccgt
     1921 tccacctgtt ccggcgacgg tgacgaccgc gaccgcacag acggtgaccg agacgcccga
     1981 cttcgtcagc ccgaagaccg acacgatcgc ggtcgatacg cttccgggcg acatcgaaga
     2041 gttgaaccgc gtcgcccagt cgcgcggcta cgtgcaggac gcgttcttcg aattcaacga
     2101 ctccacgctc acggccgctg cgcagacagc gctgacgtcg tcggcgacct ggctcaaaaa
     2161 gaacgggcag tacaacctgc tcatcgaagg tcattgcgac gaacgcggca ccgagcagta
     2221 caacctcgcc ctcggcgacc gccgcgcgaa ccaggccaaa gagtatctcg tcacactcgg
     2281 cgtcgatgca ggccgcattc gcaccgtgag ctacggcgaa gagcgtccgt tcgacccagg
     2341 tcacgacgaa tccgcgtggg cgaagaaccg ccgcgaccat ctcgtgcttg tagggaaata
     2401 gcggggaaat agcgaatgca atcgttcatc cgaatcgcgc tgcgcatcac tctgctcggg
     2461 gccgccacgc tgtcgtcggt cgcatgcgtg accagctccg acgtcgaaaa actgcagagc
     2521 cagatctccg atcttcagga gcagctcgtc caggtcaagc gtacgtcttc gagcaaggaa
     2581 gaagtgcaga acgtcaacaa gacgatcgcc gaccagacgc agatgctctt gaagtcgaac
     2641 gccacgctgg tggcgaaagt cgatcagatc gaagacaaga tgcagggcgg gcagggggcg
     2701 gtcgagcaga ccaacaaccg gctcgaccgg atcgcgcagc agctcacgca gacgcagcac
     2761 gacgtcgacg ccttgaaagc ggcaattgcc caggcctcgg ctgccgcgac ggcgaacgta
     2821 acggtgccgg ctacgtccgg cagtgagctc accgtacagc cccccgcggg gacggccgaa
     2881 gacccgatgc aggtctacca atcggcgtat cgcgactacc agcgaggcaa ctacgatctg
     2941 gccatcgccg gcttccgcga cttcgcttcg cggaacccga attcggagct ttccggcaac
     3001 gccgcctact ggatcggcga gagtctttac tcgcagaaga aatatcgaga ggcgatccag
     3061 cagtttgaca gcgtcgtcac gagatacccg aagtccgaca aggtggccgg tgcgctgcta
     3121 aagaaaggtt acggatacat cgcgctcggc gagaagtcgc agggcatcgt gcagcttcag
     3181 tatgtcgtac acgagcaccc cacctcaccc gaggcggcgc tggcacggca gaaactgaag
     3241 tcgttgggcg tcgattcgaa gtagaatacg cggctcgaaa ccgcacaaac ttacgtagga
     3301 gcaacattca tggcgaacac ccgatcggcc gaaaaacagc agcgacaggc cgaaaaagca
     3361 aacacccgca accgcgccgg caagtcgcgg ctgcgctcgg cgctcaaaaa gacccgcgcg
     3421 gcagtcgatg ccggcggcgc cgacgcgagc gtcctttcca ccggcttctc cgagatcgac
     3481 aaagctgcga agcgcggcgt gatcaagggc aacaccgccg accgctacaa atcgcggctg
     3541 gccaaagcct ccaagcgcgc cgaaaagtaa cgcgcgtccc gatccggtct ccaaacggcg
     3601 cacctcggtg cgccgttttt tttgtgcctt tttgatgcgg caatcgacgc aggagtgcga
     3661 gcaccaaggt gaggtaagtt tgtcggcacg cctatcgtcc cgcgatcgta tcgagcacgc
     3721 ctcgctgacg acgatcgagc tggtgatttt ctggggcgtg atcggggcgg tgttgtattt
     3781 gattgcggag aggggacgag gccaagcggc cggagtgtcg ctgtcctagc caccctccag
     3841 ctggtctgct catatctttc gttcgcgctc caatttggcg gcttccgtga cgagggccgt
     3901 gatgatgccg aacggcttat tcacattgtc ggatgaagca gccgtcccga ccccaactgc
     3961 agctctggca agatgagtat ggagaagaat cgcgcagcaa ttatagtagc cgatgactcc
     4021 cgctatgagc acgaccacca cggaggtcac gatcaagaag gacgctcttg ccgcgagctc
     4081 tagatcggaa gcaacgtaaa caagcgcacc tacgccaaac acaccgccaa ttgccagaat
     4141 gcgggcgata ggcttgtaag tccatgttgt ggttaacgca aatgcgatga gcaacatgat
     4201 tgccgcgcac cacgccttgg caagcccggt ccgttgggca gagtaaagcg caatgagccc
     4261 accagagacc aggaatagag ccgtgtttcg caagaagatc actaggttgc gtacggaggt
     4321 ctctgcaatc agtttttcca agtggggatt ctcgagtgaa gtgcgcagaa accaattcat
     4381 ggtggcgaat gtcgcgtcat caatgccaag ctccttcatg atcggtgaga cgtgacgcgc
     4441 ttcgaagttt gaaagtattg gaaagattct gtgagtccca ggcctgcaga aaaacgtctc
     4501 tagcgcggtc tgaacttgac tgaagagtag ccagctgaac gcgttcacgg cataaccaag
     4561 aggagtagca agaagaaaga gggttacgaa gacaaacact ttagtctccg acccgatgtg
     4621 agggtcgcca ccaaaactag cgcgaaggga ataccttgta aacaccgggt aatagtaaga
     4681 ggcagcgaac agtagaatca gtacgaagcc tgctgcgctc tccgaaagga aacggcgata
     4741 tgagatttgg atgctgtctt tggaaacgtt gaacgtactg tcggacatga gttccccctc
     4801 tcaagaggag tgtctctcca gtagattctc ctaccctggc gcctgatgtg tttccagaca
     4861 ttcgaatcct cacgtgcctt tccgggaacg ctgcgatgct cgaaagcggt gaggaaccca
     4921 cgtgaccgag gcactgcccg gctccaaagg aacgcttgat ttattcgttc atgagcgagc
     4981 gcttcaaatc actccacgac tcgccgtcgt cgggattctg ttgaagctca acccagcaac
     5041 gctcgagctc atcgcgctgc gccgctgtga tctcgacgtt ctccggatgt tcgagcatgc
     5101 tctcccagat ttcctggacc atcgcaacgc gttctgcggt cggaagttcg aggatgtggt
     5161 cgatcggtac gcggctcata gtgtgaatgt tactccctca cgtaacgatc gtctgcttcg
     5221 ccgcctgccg tttccagcga tccgttcgca ggatctcgtc gactgttgcg aaggcgatct
     5281 ccagctcttc gtccgttgtg tagaagtgcg gagagaagcg gacgccggcg ccggggcgga
     5341 agtcgcagac gatgtcctcg gcgttcagct cgtaggcgac ttccttcgca tgtggcatga
     5401 gcacggagac cgagccgccg cgtcggtctg attcgcgcgg ggtgcgcagc tcgaacccgc
     5461 gcgcatcggc gagatccatg atccgctgcg tttgtcgcag cgactttgcg cggatgacgt
     5521 tcatgtcgcc gcccgaggcc tcggcgatca ccttcggacc ttcgcgggca gcgtagagcg
     5581 cggggatggg cggtgtgccg agggcgaagc ggagggcgtc atcgcggaag cgcatcggtg
     5641 gcgcttcgaa tgcgaaggga ttcgcgtgcg ccatccagcc ggtgaagctc ggctccagct
     5701 tcgggcgcaa cgctggatcg acgtagagga acgagccccc tggtccgccg cacagccact
     5761 tcagcacgcc gccgacggcg atcggaacat cccagccggt gacgtctacc gggacgatgc
     5821 cggcggattg gaagacgtcg aggaccagcg tggcgccaac ttcgcggcag cggcgtgcga
     5881 tcgcctcgac gtcgatcatg taggacgact cgaagagaat ctggccgatc gggacaagct
     5941 gcgtccgctc gtcgatcgcc gcgaggaggc ggtcgagatc gaagccgaga cctgatgagt
     6001 cgtgcggcac gttaacgatc tccgcgccga ggcggcgggc gagacggtcg tagatatagc
     6061 ggacggaagg gaactcgcca tcgacgatga cgacgcggtt acgcggcgcc gtgtattcat
     6121 acgacgacag cacgaccgcc tcggcggtgg tgacgttcgg cagcatgact accgagccgg
     6181 gcggcgctcc gatcaacggc gcgatgatgt cgccgacctc gatcgacatc atccaccact
     6241 tgtcgcccca cgcgcgcacg ccgcgggagg cccagaggtc ggcgtactcg accagcgcct
     6301 cgcgcgtcgc ttccggcatt gcgccgagag aatgcgagac gaggtacttc gttttgttga
     6361 ggatgggaaa gcgatcgcga tactgcagaa gatggtcggt catttcaccg cgtagtcgtc
     6421 tccatagaat ttgctggtga tgctggtgtg aaagttgatc gctttcacgg gcggatattt
     6481 cggattggca atgcagggca gcacttcgag caactcgtct tcccacaaat aaaccgccat
     6541 cgggaaggag acgcgcgtct tgtttctctg ctcgggatcg cgcggcttgc tgacgcgatg
     6601 cgtcgtcgac gggaagatgt cgttgctgat gcgctgcatg tagtcgccgg tgttgaggat
     6661 gatgcttccc ggcggcgcgg cgatgcgcac ccatttcatg ttgcggcgat tgaggacctg
     6721 caggccctcg acgcgcgggg cggggaggaa cgtgaacatg tcgatgtcct cgtgaccgag
     6781 gagacgggcg gcgccggaag catcatcgga ggccgagacc ggcgggtagt agttgagacg
     6841 aagaccgaag ttcgtaccgg tcagcttcgc gtcgaagaga tgcgcgtcgc atccgaggta
     6901 ggtgaggagg ctctgcatga tgggaaggat caggcgttcg tgcgcgtgaa cgatctgttc
     6961 gaagaacgcg gggtcaggtc taaagtctaa agtgccccgc acatcatcgg atgagaagtg
     7021 gtcctgcgca ggcactttag actttagacc tgacccgact accggccaga acttcgccgg
     7081 atcttcgaac gcgcggcggc agaagaccca tccctcgacg agatcgggat gcatgttgct
     7141 cgtctctttg atcgggaagt agccttggtt gacggagccg aagcgctcgg cgcggaaacg
     7201 catcttctct tcgagcgtca aggtgctgaa gatctcttcg atcctccgct ccgcctcggc
     7261 ataaagactc ggatcgacac cgtgcccttc gatgatggcg aagccgatct cgtgcagcgc
     7321 gtcgccgagt tgcgtggcga aagctcgctt gtcatcggcg ttaccgtgga gaaagcggct
     7381 gacgtcgacg gtgcggactt ggaagtcgtc gtcgaactga ttctcatcca gcccttcagc
     7441 gagctggtag gtctggtgct tctcgacctg gtcgtaacgg atgaagtcct ggttggtggc
     7501 gcggactcct tcttccttgc tggtcatgca cggagcataa tcccacggaa tggttgcaaa
     7561 actcgatggt ggttgtttgg tggccgggct gaaagagggc gagccggtcg tcgacggcgc
     7621 tcttcgcatc tggaaacatt tcggccgcga ggcgggcgcg aaggcgatct cgctgcgcgt
     7681 gctggagttg ccggcaggtg agagggcgac gctgcggaac gaggtggcgg atgaggtgct
     7741 ttacgtcatc gctggttcgg gagctgcggg tgattcggcg atctcggtcg acagtggcat
     7801 ttacctgccg ccgtctgcat cattagatat tgtggctggc gagacgctga ctcttgtcag
     7861 ctcgcagtgt ccggatgttg acggtggtca aatcgccgcc gggccggcgg cgggacagcc
     7921 ggcgggccgc cggcgctacg ggggcgcgct ggttgtggcg atgcgcgatc gccccgttga
     7981 gcgcacggga gatcgctggt atcgcgaaat catcaacgcc ggcgacggca gcatccaagt
     8041 cacgcagttc gtcggtggca ttccgcccgg ccgttcgccg gatcactacc atctgtacga
     8101 agaggtgatc gtgatcctcg agggcaccgg tgtcatgtgg gcaggcacgt cgaatacgcc
     8161 gatcgagacc ggctcgtgca tcttcctccc gcacggtcag gtgcattgcg tcgagaaccg
     8221 aggcgagggc gagatgcggt tgcttggtat gttctaccct gcggggagtc cggcggtcag
     8281 atatggcgca aacgggaaac cataggcacc tcgctttcgt caaaagtaca tgcgctggac
     8341 cgttctcgca cttctcgtcc tctcttcgtc gacacttgct gccgatacgc acgtcacgct
     8401 gacaggggcc tccgaggggg cggaggtctg tcgatttcag gcgcgtgatc gggagaaacc
     8461 gatcgagcgt tggttgagcg cgcaggcggt tacctgcgtg gcgacggatg cggcgctgac
     8521 ctttccgccc gggctctgga acgtctttgc gcgggcgaag gggatggtgt cagttgaacc
     8581 gatgctcgtt gacggcaaca gcgcgcctgc gaatctggcg ctttcactcg ttcccgcggc
     8641 gacggtcgtg gtgcaacttc cggcggaagc gaggggcgtg ctgtacgcgc cgaaacatgc
     8701 catcgcattc cccgcgtcgg agcggacgac ggttccggca ggtgaggagc tctggctcat
     8761 cgtcatcgtg aaggcagccc ctgtcgctat cgtccccata gcgtcgctcg agcctgggat
     8821 cgagcgcgtt gtcgatgcgc gcgctatcaa cgacgcgccc gccgttctcg gctggattca
     8881 cgtttccgaa gctgaccgtg ccgcgatcaa gacggcgcgt ggcgtccaac tgccgcacat
     8941 cggaatcacc gccacgggca aggaagtcgt cgccgcctcg ctacccgtcc aggacgcgtt
     9001 gaacggcgcg ttcgttctgt ttcgcggcct atccgcgggg agcgccgatc ttcggctcga
     9061 tgggcggggg tggcttccgt tccggcgctc gctccgcgtc gaaccgaagc cggtgacgct
     9121 gctgcgcgaa ccgatcgccg cccgtgcgtc gacgacgatc atcgtcaact ggagcacgtc
     9181 cggcgatctc gccgcgctcg atcgctcact cggttcgtgc gagacgccga aggaggctcc
     9241 gtcattagag ctcaccatcg ccgcgtgtcc ggaaccaaag ccgggcaagt cactggaccc
     9301 ggcaacgtgt tccgtcattc gcaaagaaac gcttcgcaac gacttgtcct acggatcggt
     9361 ccgggtcgac gaggttccgc ccggtatgta ccgcgccgag ctgcgtttcg gccggctgcc
     9421 tccgttcgat gtcatggagg atgtgccgcc gctgcagcag cggcccatgg cgccgttgca
     9481 ggccaagtat ttcgaagcgt acggcagtct cacgcgcggc ggtgaaccgc tgcacgaaga
     9541 cgcccgcatc ctttttccgc acgacggcgt cggattcgcg ccgcgcgata ccggggagta
     9601 tcgcggcgtg gtcaaggagg ggtttcgctc ggatgcgaag atcgacgtcg tgacctgcag
     9661 cggcaagcgg gcatatgtca tcaccgaccg ggaaatgaat gtctgggcgc gaacgcggtt
     9721 cgacatcgat attcccgata acacgctcac cgtcacggtc gtcgacacgt tcacacaaag
     9781 gccgctgcct ccggcaacgc tcaagtacgt cgtcatgtcg ctccagggac gatttcctct
     9841 gctcactcgc gaggtcagcc agagcgatga gggcgatgaa cggggcaagc aagtcgcggg
     9901 acagttcgtg atcaagggac tgccggagcg caagctgctt ctcaccgtga gctgccccgg
     9961 ctacaagaag aaggagctcg atccgttctc gatgacgaag agcgagaaaa aggagatcga
    10021 cgtcgatctc gttcccctcg gcgggtcgga agcgagggtc atctcctccc gtcccttcgc
    10081 aaacggaatt atcttctggt tctcatcgac gggcgcggag atggagcgcg ccgagctcgc
    10141 tcccgacgga acgttccatt tcgagcagac gcactatcgc gacgagacca tgacggtcgt
    10201 ctcggcctcg catccgctgt ggatcctgcg tgctccgccg gtcgagaaag cgacgccgct
    10261 gcaggtcaag tttcccgacg ccgcgccgca gcgtgatgtc gaggtcacga tctacaacat
    10321 gccgccccgg ctgctgacgc cgatcggtgt ggccatcggc ggactgcgtg ttccgcagcc
    10381 ggcgctggcg cagcatcttg cgctccgagg aaccgcgccg ctagtcaagg gagggggacc
    10441 gttgctcatc cccggcctcg ccgagagcgg cccgatcgac attctgcgag ggccgccggg
    10501 tgcgcagcgg ctccagcagt cgcttccgct tccgccgctc atggatccat cgcttcgcaa
    10561 cttcgctccc gtggccacgc aacggctcga gccggggagc ggggcggtgg cattcgacgg
    10621 agcggcgaag tagcgaacga ctgccacaaa gacgcaaagg atggcttcac cccgtgtcct
    10681 ttgtgtctct gtggttcatt tttttggccg aggttagctt ccgctccaat cgccgcgcac
    10741 gacgagcacc ggcttcccgc catccgttcg cgcgatgcgc gtgagcgtca cgacgtagac
    10801 cggcaacgat gtctcgcgcg ccacgcgggc atcgatgttc gacggcagcg gcagccagtt
    10861 cggatgggta tggacgatgg cgacagtgcc ggcgggaatc ggaccacgcc aggaagcgcg
    10921 gttcagctct ccgttcgccc gccagcgaag aaagaacgtg gcgccgtgcg cgtcgcggat
    10981 caggaacgcg gcttcttcgg tgttcacgca accgtagcga gcattgcgca acagttcggt
    11041 gagcacgcca cggactgcgg gatcgtgaag atcgatcgcg gtgaacgtga gaacgaaggc
    11101 ggcgatgacg ttcagcatca tgcctgcttg aacgatcggg aacgggaaag gttatcggcg
    11161 cgagacgcgt actcccatca ctcgaagtcg gttacacgag gggcgttctg gagaccgtga
    11221 agacgatcga ggacgtgcga gacgcgttcg cgctcctcac ctggcggaag gcgctccagt
    11281 gttccggcca cactctcccg aatcgtgtgg cggccatcct cgtccgcgtg gcggaacttg
    11341 gtcgccagca cgtaggcaat gatcgacgcc ttcgattccg acagcgccac gagatcggga
    11401 aggtcgcgca gcaggcggtc ggtgggacgc gcttcgagat ctttgacgaa ggcctccagc
    11461 tcgctgaagg ttttcgcgtc gatcgctttc atcacgatcg atgacgtcgg gatcttgtcg
    11521 ttcttgtcag gtgccataag tgcgtcgatc gtacatgaat cgcgcctact cctgatttcg
    11581 caccgccgtc agcctgagaa agaacggtta cgaatcgctg tggttacttg gtctgatgct
    11641 ttccggtgac gggaggctcc ctgcgcgaag tttgatcgtg aaacggattt gcgactaccg
    11701 cttcttcttc agcgctttcc cgatcgtgag cttgtatccg tcgggatcgt ccagggagat
    11761 ttcgcggtag ccccacgatt gatccgtcgg ctcctgcgcc agcgttccgc cggcggcctt
    11821 gattcgtgct gcgatcttgt cgatgtcctg atccgtttcg caatacaggc gcactccgac
    11881 gcctttgaca cggtcgcgtc ctttctgcca atcgtcctgt ccgatcatga acagcacatc
    11941 gcccgcggag agctcgacgc cgcgcagcac tccatccatc tcccatcggt cgccgacggt
    12001 gaagccgagg acgtcgcgat accaggccag actcttgttg atgtcgttga cggtgaagct
    12061 gggactcgcg gagaggagac ggagaccgcc gctatccttc ttcgcgctcg ccgtcttcgc
    12121 agcgctcttc ttcgccgctt ttttcgcggc gggtttggcg acgcgcttcg cgctcttcgc
    12181 cttcgcagag ggcttcttgg ttttgcccgt tttctttgtg gccatggggt tgtcctcctt
    12241 cgttgcttga ggctgcaact atcgctcccg ggaatgattg ccgtcaacga ggggggatct
    12301 tgtcgctcgt ttgtgcgacg atagcggaag acaatttgag gtttagcgat gccgaccgcc
    12361 acgatcacac tggatgtcac cttaaaagcc cgtctcgcag agcttgccaa ccagacagga
    12421 caaggtgtcg atgatttcgt tcaagcattt cttcgacgtg ttgcggatgc cgacatacgg
    12481 tttgatcgag gcgttccggt gttcccgccg cgacccggtg cccctccgct aaccgtcgaa
    12541 gatgttgatc gaatcgcaaa cggggacggg ggataggccc gccccgtggc ccgtctcctc
    12601 gacgtgaacg tcctcgttgc gctgttcata cctgaacatg aacatcacgc cgcagcgatg
    12661 aagtggttct cgaatgaggc tgccatcgca ggatgggcaa cgtgtgtaat aaccgagctg
    12721 ggcgcgatta gagtctgtgc gcaactcccg ggcggcctgt ggcctccgga agcgaccgcg
    12781 gatcgcttgc tcatgttgac gatctcgaac cgcggctacg tttggtggcc ggaaacagtg
    12841 tcgcctgcag tagtacccga ggttcgatca gcgggcaccg cgaagcacgt aacggaccgt
    12901 tatctgctcg gtttggcacg gcaaaacggc gggcaggtag tcaccttcga tcgggcgctg
    12961 gcggtcgcag gtggagatga tgtcatcggt ctgttgcctg tgggcgggga ggtaccgtga
    13021 atgccatgag cttggctcag cttctgaagg agatcaaaac gctgccgacc tgggaacgca
    13081 aacgtctcct gcgagaagtc ctcgtcaacc agaaatctca cagcgtgtcg gtcgattcgg
    13141 tttcgatggg aacgaagaat cccttctccg ggcaggcgag cagccaatcg aggacacctt
    13201 cgttcggctt tcgtgcgcgg cgcaccaaac ggtattggcc gcgatcgagc cgttcgacga
    13261 tgaactcatc tcccggcacg atgccgtcct gcttacgcag cgcggctggc agcacgattc
    13321 tgcccttgga ggaaacgatc gttttcaaca tgcgccgttg catttcgttc ttcgtgtgat
    13381 cctatttctt cggcgtcgcc accaccagat cgctcagttt tcccagtcct gaatgcgcca
    13441 ggtagaccgt gccgtcgctc gtgaccgccg gattgcgcgc gcccgtgtgc accggtacct
    13501 tggccacgat cgacaagatc cacttggcgt cggcgtgtgc gaccgtgagc aaccctgctt
    13561 tcgccgcgcc gatatagacc atgtgcgtgg cggatgcgta atcgaggtcg tcggcgccgt
    13621 ctccggtatc gatcgatgag agcaccgccc cgtcatgccc cacatccatc acctcggtgc
    13681 gcgcgctgca ggcgatgaag agatgtcccg ccttctcatc gacgcgcaat ccgtgaggac
    13741 cgtcggcgcc acacgtcgat ttccaagtgg caactgtctt gtgcgttccc agatcgatcg
    13801 ccagcgtcac gtctttgtct tccatgttcg tgtagaagcg gccgcgcttg ccgtcgaccg
    13861 cgaagccttc cgggttgccc tcgaaggtca gtttcgcttt ttcattcagt gttgccgcat
    13921 caaggatgcg gatcgacttg tcgcgcggcg tcgtcaccca cacttccttc atgcccgcga
    13981 cgtaggcaag tccgtcaggc atcgagtcga ccttgtggca ggtgccgcga acgagcgtct
    14041 tcgggtttac ggcgcaaacg gtggagtcgc cgcgattgcc gatgtagatc gtgccgtcgc
    14101 cgatcgtcac ggagctgggg ccaacgaccc gcttgcgatc gccggtaccc atctcagccg
    14161 tcggaaagcc ggtgatctga cgcaccttcc cggtggccgg atcgacgacg tcaacggcgc
    14221 cggtgttccc cgcgggaacc cacacggagc gcgtcgccgg atcaaagatg atgtagtcca
    14281 tcccgatgcc ggcgtcgctg gcgcccggca gtgcctgcat cgacaccgtg taggtggggt
    14341 cggcggcgta aagggtttga gcggcgaaga gaaacgcgcc gcaaagggca acgcgccaag
    14401 cggcgattcg tagattcgtg atgttcatga tggctcctat tcgtgcgggg aaagtcaggc
    14461 ggaacgcgat tctagcagcg gttcgtggag tgtaattacc accacagaga cacagagttc
    14521 acagaggtct ctgtgtcctc tgtgtctctg tggt
//
LOCUS       DATLVW010000030         4466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_167208, whole genome shotgun sequence.
ACCESSION   DATLVW010000030 DATLVW010000000
VERSION     DATLVW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167208"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VNN25_01015"
     CDS             complement(<1..294)
                     /locus_tag="VNN25_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90131.1"
                     /translation="MIRTIVAILTVLPAAFAARAQSSAEVNAGIQYNFSSPGARSLGR
                     AGAFIADASDATAAYANPAGLVNLPTREMSIEGRASEFVNAYSASGHAFGPPSG"
     gene            complement(291..992)
                     /locus_tag="VNN25_01020"
     CDS             complement(291..992)
                     /locus_tag="VNN25_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXH90132.1"
                     /translation="MYKTGIRVAFTMPRVALLAILLAALPASLSALCHQPPVPKIDWV
                     GTLTGCSPGTGPCAAYEPINFFAKTADGSSFEACDHFDWDFGDGSPHSSLQNPTHIFA
                     FPGSFPVSLLITNDTGLFESDARFVIVAATVLPAIVEFKATPNQVTPGQTTVFSWNTA
                     NTTRGVRIENVHSSSVPAVLLVTIQQSIGTYSFIPNATMAYTLTAFGDAAQITSPAPI
                     TVEVVGASRGRAVRH"
     gene            complement(1157..2689)
                     /locus_tag="VNN25_01025"
     CDS             complement(1157..2689)
                     /locus_tag="VNN25_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH90133.1"
                     /translation="MHIQHLKEGGFRYIVVSDSTWYVQLASLGDGSIHSEASHRDKEG
                     TERLSPSVCTRLLRLGWLRSEAPAQGATRRARAFLRRWPRTTTSGEIAEGSVKTLRDV
                     FGIASPASLTIRRSEFSERSVPTIKKSGEWVSPGNRAQPPKNPNLHLPAGSIVRNAHS
                     GRQYRVENLLGSGGFGAAYRATQVSGSRLSGGCVLKIATDPQGWHREAYFGELLRGEP
                     GIVSVYETFAWVPQGEHRRPLYCLVSELAEGGDLARHLRERPVPWTESRARREITRLA
                     RAVKLLHASGAVHRDITPGNVLVTPGGVLKLGDFGIALHRAGSRNVAADAFAPRFAPL
                     PIRGRETVSWKPADDVYQLGQLFAFMLIGRAHATVTPRDVKTLSCSAEVKAVIQRCIG
                     ERRRRFANAEEMLGALGSANVPAPRQRVVRSLLGKRVVFTGTMDILRAKAKRLVKKAG
                     GTVENNISRATDIVVVGQHSPNWKADKKGRKLLDVDRERELGHDIAVISERRFRNLVG
                     LG"
     gene            complement(2855..3061)
                     /locus_tag="VNN25_01030"
     CDS             complement(2855..3061)
                     /locus_tag="VNN25_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH90134.1"
                     /translation="MKQISGKDLCRIVERHGWMLLRVHGSHHIYGRGGSIVRLSIPVH
                     GNSSVKPGLLRHLAKLAEIPEDEL"
     gene            complement(3058..3261)
                     /locus_tag="VNN25_01035"
     CDS             complement(3058..3261)
                     /locus_tag="VNN25_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH90135.1"
                     /translation="MKVKVVIHEEAEGGYWAEVPAIPGCATQGETFEELLQNLYEAVE
                     GCLSVDLDHPSDEKSARIIEIAV"
     gene            complement(3369..>4466)
                     /locus_tag="VNN25_01040"
     CDS             complement(3369..>4466)
                     /locus_tag="VNN25_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH90136.1"
                     /translation="GLWDMHVHLMIDGHADYAHWFKKYPSMYRKVIMPASAHQLLMAG
                     VTSARDLGAPLDDILAVRDAINRGEIPGPTMYVAGPFIQHEPYPGEEDSRWGVKGAED
                     ARAKVRRIAEAGVNEVKMIDQDLMTMDEVRAIVDEAHKHGLTVVAHAHRPEEIRRGLA
                     AGVDCFEHTGLATAPEYPPDIMEKLRERTAKMSLGPLFWTPTVEGLWNYESLRDNPEP
                     LDSDSWHLGLPDEVVDDIRQSLKHPGQLPYYQITPQRRPTLKRKIEQLRSAGVVLLVG
                     TDSGIPMNFHSQSTWNELDVWVRKIGIPAMDAIRSATYWPSVLMKVSKDVGTVSEGKF
                     ADIIAVKGDVLEHIDLLSRVDLVMKHGKRMK"
BASE COUNT          872 a         1443 c         1291 g          860 t
ORIGIN      
        1 accactcggc ggaccaaacg catgaccgct agcgctgtac gcgttgacga actcggaagc
       61 gcgtccctcg atcgacatct cgcgcgtcgg aagattgacc aatccggccg gattggcgta
      121 cgcggcggtg gcgtcgctcg cgtcggcgat gaatgctccc gcccggccca gactgcgcgc
      181 acccggactg gagaagttgt attgaatccc ggcgttcacc tccgcggacg actgcgcgcg
      241 cgcagcaaag gccgccggca acaccgtcag gatcgcaaca atcgttctga tcaatgccgc
      301 accgcacgcc ctctcgacgc gccaacaacc tcgaccgtga tcggcgccgg tgacgtgatc
      361 tgcgccgcat cgccgaaagc cgtgagcgtg taagccatcg tcgcattcgg gatgaacgag
      421 tacgttccaa tggactgttg aatcgtcacc aggagcaccg ccggtacgga ggatgaatgt
      481 acgttctcga tgcggacgcc cctggtcgtg ttcgctgtgt tccagctgaa aacagtggtt
      541 tgtcccggcg tcacttgatt cggagtcgcc ttaaactcga cgatggctgg caagacggtg
      601 gccgccacga tcacaaagcg cgcatcgctt tcaaagagtc ccgtgtcgtt ggtgatcaac
      661 agggaaacgg ggaagctccc cgggaacgca aagatgtgcg tgggattctg cagcgaggag
      721 tgcggactac cgtccccaaa gtcccaatcg aagtggtcgc acgcttcgaa cgaactgccg
      781 tccgcggttt tggcgaagaa attgatcggc tcatacgccg cgcatgggcc ggtgcccgga
      841 gaacagcccg tcagcgtccc gacccagtcg attttcggta ccggaggctg atggcacaaa
      901 gcactcaatg atgctggtaa cgcagcgagc agaattgcga gaagcgcgac gcgaggcatc
      961 gtgaatgcca cgcgaattcc tgttttgtac actgttcctg aattataccc aatgtgtcaa
     1021 acgctcgcaa agttccagaa actcgccgag aacccgaaag ggctcgacga catgggcgtg
     1081 aatgtgacgt acaagaggta ctgcggtggc gccagccacc gctttcaact cgcggttcga
     1141 ttcgacgcga acttgattat cccaacccaa cgagattccg gaatcttcgc tccgagatca
     1201 ccgcgatgtc gtgaccaagc tcgcgctcac gatcgacgtc gaggagcttt cgtcccttct
     1261 tgtccgcttt ccaattgggc gaatgctggc cgaccacgac gatgtcggtc gcgcggctga
     1321 tgttgttctc gaccgtcccc cctgccttct tcaccagcct ctttgctttc gctctgagga
     1381 tgtccatggt tccggtgaag acgacgcgct tgccgagcaa ggagcgcacg actcgttgcc
     1441 gcggcgccgg aacgttcgcg ctacccaacg cgcccagcat ctcctccgcg ttggcgaagc
     1501 gtcttctccg ctcgccgatg caacgctgga tcaccgcctt cacctccgcg ctgcacgaaa
     1561 gcgtcttcac gtcgcgggga gtcacggtcg catgagcacg gccgatgagc atgaacgcga
     1621 acagttggcc gagttggtaa acatcgtccg ccggtttcca cgaaacagtc tcccgacctc
     1681 tgatgggcag cggcgcaaag cggggtgcaa aggcatcggc ggcgacattt ctgctgccgg
     1741 cacgatgaag agcgatgccg aaatcgccga gcttcaacac tccgccgggc gtgaccagca
     1801 cgtttccggg agtgatgtcg cgatggaccg caccggacgc atgaagcaac ttgacggcac
     1861 gcgccagacg cgtgatctca cgccgcgctc tcgattccgt ccaaggcacg ggacgttcgc
     1921 ggaggtgacg agcgagatcg ccgccttccg caagctccga cacgagacag tagagcggcc
     1981 ttcgatgttc gccctgcggc acccatgcaa acgtctcgta cacgctgacg atgcccggct
     2041 cccctcgcaa cagctcgccg aaatacgctt cacggtgcca gccctgcgga tcggtggcga
     2101 tcttcagaac acagccgccc gacaatcggg acccgctcac ttgcgtcgcg cgatacgcag
     2161 cgccgaagcc gccgctgccg aggaggtttt ctacgcgata ttggcggccg ctgtgcgcgt
     2221 tcctgacaat cgatccggcc ggcagatgca ggttggggtt cttcggcggt tgggcgcggt
     2281 ttcccggcga cacccattcg ccggacttct tgatcgtcgg aacggaacgc tcggaaaact
     2341 cagagcgccg tatcgtgagc gacgcgggcg acgcgatccc gaagacatcg cgcaacgtct
     2401 tgaccgaccc ttcggcgatc tcgccgctcg ttgtcgtgcg cggccatcgc cgcaggaacg
     2461 ccctggcccg ccgcgtcgct ccctgagccg gcgcttccga tctgagccat cccaaccgca
     2521 gcaaccgcgt gcagactgat gggctcagtc gctcagtacc ttccttgtcg cgatgactcg
     2581 cctcgctatg aatactcccg tcgccgagcg atgcgagctg cacataccaa gtcgaatccg
     2641 aaacgacgat gtagcggaag ccgccctctt tgaggtgctg gatgtgcacg gaaagcgcgt
     2701 ccgtgagcgc atcccagctt tcgagccgaa agcgacttgc tcctgaacgc caagaggttc
     2761 ccgccatagg tttcccaatg ataaagcgga ggcggaagga gtctcacacc aacgatcgag
     2821 gctctcgctc tgaggtttca tggacgctcc ctcgctacag ttcgtcctca gggatctcgg
     2881 ccagctttgc cagatggcgc agcaggcccg gcttgacaga cgagtttccg tgcactggga
     2941 tcgaaagtct cacgatcgat ccgccgcggc cgtagatatg gtgactgcca tggactcgaa
     3001 gcagcatcca gccatggcgc tccacaatgc ggcagaggtc tttccccgag atctgcttca
     3061 aacggcgatc tcgatgattc gagcagactt ctcgtcagac ggatgatcga ggtccacgga
     3121 cagacatcct tcgacagcct cgtaaaggtt ctgaagaagt tcctcgaaag tctctccctg
     3181 cgttgcgcag ccgggaatgg ctggaacttc ggcccaatag ccgccttctg cttcttcgtg
     3241 aatcacgact tttactttca tttttcggcc ctccgattag aagacgcctc aggagtgtcg
     3301 cagatcccgg caggattcgg aactcacgtt cgtcagcatc ggctcgcgga accgtcgagt
     3361 acccccgatc acttcatccg cttcccgtgc ttcatcacca ggtcgacgcg cgaaagcaga
     3421 tcgatgtgct cgagcacgtc gcccttcacc gcgatgatgt cggcgaactt cccttcactg
     3481 accgtcccca cgtccttcga cactttcatg agcaccgatg gccagtaggt cgcgctgcgg
     3541 atcgcgtcca tcgccgggat tccgatcttg cggacccaga cgtctagctc gttccatgtc
     3601 gattgcgaat gaaaattcat cgggatcccg ctgtccgtac cgaccagcag cacaacgcct
     3661 gcactgcgaa gctgctcgat cttgcgcttc agcgtcggcc ggcgctgcgg cgtgatctgg
     3721 taatacggaa gctgaccagg atgcttcagc gattgacgga tgtcgtcgac gacttcgtcc
     3781 ggcaatccga ggtgccagga gtcggagtcg aggggctcgg gattgtcgcg cagcgattcg
     3841 taattccaga gaccttccac cgtcggcgtc cagaagagcg ggccgagact catctttgcg
     3901 gtgcgctcgc gcagcttctc catgatgtcc ggcggatact cgggggcagt tgccagtccg
     3961 gtgtgctcga agcagtccac acccgcggcc agaccgcgcc ggatctcctc gggacgatgc
     4021 gcatgcgcaa cgacggtcaa gccgtgtttg tgcgcctcat cgacgatggc cctgacctca
     4081 tccatcgtca tcagatcctg atcgatcatc ttcacttcgt tgacgcccgc ctcggcaatg
     4141 cggcgaacct tcgcccgcgc gtcctccgca cccttcacac cccagcgcga atcttcctct
     4201 cccggatacg gctcgtgctg gatgaaggga cccgcgacat acatcgtcgg accgggaatc
     4261 tcgccgcggt tgatggcatc acggacagca aggatgtcgt ccagcggcgc accgagatcg
     4321 cgcgcactcg tcacccctgc catcaacaac tgatgcgccg aggccggcat gatcaccttg
     4381 cgatacatcg acggatactt cttgaaccag tgcgcgtagt cggcatgccc gtcgatcatc
     4441 agatggacgt gcatgtccca aagccc
//
LOCUS       DATLVW010000031        26521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_1414, whole genome shotgun sequence.
ACCESSION   DATLVW010000031 DATLVW010000000
VERSION     DATLVW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 26521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VNN25_01045"
     CDS             <1..729
                     /locus_tag="VNN25_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90137.1"
                     /translation="FGIRGLMPALWLEPIRDLPRFMMIAVAFGVFVVTAGLVLNIINR
                     WRSGDMREALYGPRGATWAMLYWIILVLLARAFVPATVRIPGSVIAYVGIGAIVVIAG
                     AGPLMKWLGRDGESRPRPAVRATPWWLRALESSVELVDTLFSFFANTISFVRIAAFAA
                     VHAGVFIAIFALADTIAAMRFGGVLSIVVHVAGNVLVILLEGLTVSVQVLRLEYYEFF
                     GKFFRGGGERYAPLTLRPGSERHA"
     gene            722..1057
                     /locus_tag="VNN25_01050"
     CDS             722..1057
                     /locus_tag="VNN25_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HXH90138.1"
                     /translation="MRKKLLWTIAAIVPVVAALVVVPLFAAPEAARVAAPVPPEVVRW
                     GFVAAAIAAGLAALGAAYAVAVVGAAAMGAIAEKPETAGRALIFVGLAEGIAIYGLII
                     AIMILGRLG"
     gene            1054..1257
                     /locus_tag="VNN25_01055"
     CDS             1054..1257
                     /locus_tag="VNN25_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HXH90139.1"
                     /translation="MKPFVIGSPEDVVGFALAGIDGVICATREEADAALTRVDAGVLV
                     IMSADFARAPSPGNLIVALPARM"
     gene            1257..1817
                     /locus_tag="VNN25_01060"
     CDS             1257..1817
                     /locus_tag="VNN25_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HXH90140.1"
                     /translation="MQTLGSPASIIAAIHEEADAEAERIDAATAVELASIRSDAAAAN
                     VGIADREERLSAARRMNEERMAKQEWDGRRAVIEQREKWIQRVSAAARTKWSSGPPAT
                     RREQLNALIREAVSRFPRGTCEVAVCTADRTLVDVSDDIKVTTAPIAGGCIVTIGDVS
                     FDNSFEARARRQEPEWRNALSGMYTR"
     gene            1814..3481
                     /locus_tag="VNN25_01065"
     CDS             1814..3481
                     /locus_tag="VNN25_01065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HXH90141.1"
                     /translation="MSDIAGRITEISGWVVRARANVPLALREVVLVGTERLIGEVIAL
                     DRAEATIQVYEETEGVAPGDPLFATGDPLSIELGPGLLGGIFDGMQRPLRSLADAEGD
                     FIGRGAQMPSLDREKLWDFIPEVEAGREVHEGDVLGRVLETPVLAHPIAVPPGISGKL
                     AWIASAGPRRIMDAIARLDDGRELTMLQKWRARVRRPVREHLVPDAPLITGQRVLDTF
                     FPLVRGGTAAMPGGFGTGKTVTQHQLCRWAEADVIIFVGCGERGNEITQLIDELPRLV
                     DPRTKRLLAERSILIANTSNMPVSAREASLFTGVTIGEYYRDLGYDVLLLADSLSRWA
                     EALREISGRLGEMPAEEGYPPYLSSALAAFFERAGSVVTLSGGHGSLTIIGAISPPAG
                     DLTEPVTRHAARFVRTFWTLDRQLAGARVFPAVSTRSSYSEVPPGVTSWWSENVSPEW
                     AATRDGALRFLEEASLIEETARLVGVDSLPERERYLLQLSSAFQEGFLQQDAYDASDA
                     CCAPARQMTLLETFMRARSEGLEQIGKGAPVSTDFAALQRMKTEPPS"
     gene            3478..4854
                     /locus_tag="VNN25_01070"
     CDS             3478..4854
                     /locus_tag="VNN25_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HXH90142.1"
                     /translation="MTTAGLERRGLAELRGPLAFVEGVSGIAFHEFVDLVGSDGETRP
                     GRVLSTTEKGAIVEVFGVTDGLQLDASRVRFHGRPMRFSVGLELLGRVFDGLGRPRDG
                     LPPPACEERRTINGSPINPCRRAYPRDFLETGISSIDALNSIVLGQKIPIFTESGLAH
                     DDLAMQIIRQARAPGVERFAVVFAALGLPRDTAERYLTGLQASGARGRLVAFLNLADD
                     PAAERLITPRLALTAAEYLAFDAGYHVLVVLHDITNYGEALREISAARGEVPSRKGYP
                     GYLYSDLASLFERAGRIAGRPGSITQIPIVTLPSGDLTHPIPDLTGYVTEGQIVLDRE
                     IARRGVYPPVAVLSSLSRLMSDGIGEGRTRDDHSDVAKQLYAAVAHVQRARGLASIIG
                     VEELTEIERTYLRFGERFERELLAQAGDESRTIAQTLDRAWSVLRELPRAEMIRVRAP
                     ILEKYFAS"
     gene            4851..5459
                     /locus_tag="VNN25_01075"
     CDS             4851..5459
                     /locus_tag="VNN25_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HXH90143.1"
                     /translation="MIGAATRSRLFELRRDRSAARHSAELLDRKREVLLREIARRERL
                     RNDLRAVVAAKYAEAQLRLRVARVELGMRGVEAVALAQPVRQIIARRDTSVMGVRIPQ
                     LRATVEPYRAFYGAAATTESLDECGAAFTDLVPHLVALAQEEIAVMRLSAAMRKTTKL
                     VNALQRVVLPRIEKEIRAIVDGIEEDERDESVRRRVGRAERL"
     gene            5819..6961
                     /locus_tag="VNN25_01080"
     CDS             5819..6961
                     /locus_tag="VNN25_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HXH90144.1"
                     /translation="MAQKKTTAGTSRSRIVKQPVAVASARKPSRTAASPAALLSEVRE
                     LILQTRKTVAQGVNSALVLLYWQIGQRIRIDILKEKRAGYGEQIFHSLSGKLTAEFGG
                     GYTERNLANMVRCAEVFPDRNILHTLCAKLSWSHFRRLIYVDDPLKREFYAEMCRVEQ
                     WSVRTLHERIQSMLFERTALSKKPDSLIAAELKMLRDEDKLSPDLVFRDPYVLDFLGL
                     KDTFGENDLEAAILREIEAFILEIGVGFCFVERQKRMQIDGRDYYLDLLFYHRKLRRL
                     VAVELKVGDFEAADKGQMELYLNWLKRHERERDEDEPLGMILCAGKSQEHVELLELQK
                     SGIHLASYLTTLLPWKQLETKLREAVKLARQRLQEPALGVAQGRPS"
     gene            7234..9291
                     /locus_tag="VNN25_01085"
     CDS             7234..9291
                     /locus_tag="VNN25_01085"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90145.1"
                     /translation="MFAVTYVVPADRVEVERSNAIIVGRVLRSHVERSPQFGIETVTD
                     VVLEETVKGSVESLFQIHEPGGVLDDQAYVIPGVPAFIVGDRVLLFLYQRENGEYTVN
                     DFELGSFHFTKDAAGRELVIRNESEVNGWDLDGTPHKEPHRAAQQFINYIRGIVRGEE
                     VGEDYVVATLPLAGVTETVKAEPGSLHPITTAAFTAASYTVDLSAGLGARWNIFPSTV
                     SWNRGNSETGVLGNGASQIATAFASWNGGGAHYVLASANPNPNGIMDALDGINNIVFE
                     KNLTGAGLQPYSCTKGGALGMGGMHSALFGGGTHVFHGETFGTTVEVDVSMNQGLGAC
                     TLAQLSSEQFNTTVTHEVGHTLGFRHSDQNRTQNALCTTDPSLDCSNAALMDHLLLVG
                     LNGQLQPWDSSAISIVYGNGPACTPPSIAQQPGGSTITSGNSAFLSVTATGTPPLTYQ
                     WFAGSSGNTSTPVNGAGATISVGPAVTTSYWVRVTGQCAPAADSAAATITVNPANCPT
                     VVLGTPQAIPVNGGFQLSINTSGGNSFTYLWFLGTSPGLGSQIGAGNPLLVNPAQTAS
                     YWCRVTNNCSNTADSAVVTVTIVPCSAPQIVNQPQNQTALIGTTASLTVGANGTAPTI
                     TWFQGPSGNTSTPVGSGKTITSPVLTQTTQFWARITNSCGSIDSNAGTITVEVARHRA
                     ARR"
     gene            complement(9396..10100)
                     /locus_tag="VNN25_01090"
     CDS             complement(9396..10100)
                     /locus_tag="VNN25_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90146.1"
                     /translation="MKRNARTIAFTSFAVVIVLAAALAAQAQDVKTPYPSMAPLDQYM
                     MERDAEIALARSAAPDSISRDAEVMVLGEHGYETAVKGKNGFVCLVERSWASPLDHPD
                     FWNPKLRAPNCFNAVASRSYLPLTVRKTTVILATRSKVEMVKDIKAAIDKKELPPPEL
                     GAMCYMMSKQGYLNDAAGHWHPHLMFFLPLTDPATWGAGLPGAPLLSASDDLGRMTVF
                     MVPVGKWSDGTAADQH"
     gene            complement(10097..10891)
                     /locus_tag="VNN25_01095"
     CDS             complement(10097..10891)
                     /locus_tag="VNN25_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90147.1"
                     /translation="MSELDSPETSSLLLKTGRLLFALTVVASGIYQLVTARWVNLVPV
                     NPAHLPPLWLAYLFGVLLVLIGAGLLVRKTVLPAAIALATLLLVLFFGFSLRVAIPHA
                     SMGYVWVDPLMILAILGGVSLVVYPREGSSASSADRVFEKATHFVPWALGAFLAYCGA
                     LHFPYAKYVAGLIPPWIPGHMFWTYFAAVALIAGGIGVVVPQTARMAAMLSGVMLFSW
                     FFLVHIPLAINTHTVSEVSRGFQALQDSAVAFMLAGTVHIRKRSDQ"
     gene            complement(11025..11891)
                     /locus_tag="VNN25_01100"
     CDS             complement(11025..11891)
                     /locus_tag="VNN25_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013971850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXH90148.1"
                     /translation="MSRTLLAVLAVLVAMVSFQTGASLAKQLFPLVGPVGTTTLRLGF
                     GALILCAIWRPWRQKLTRAELRTIAIYGMAMGGMNLSFYIALKTIPLGIGVAVEFTGP
                     LAVAVIASRRGIDFVWAALAAAGIVLILPIFRGSPSVDIHGVLWALLAGACWGAYILV
                     GQRAGASVHGGTATALGMATGALLVLPFGVAVVGKPLLNPAILPIGIAVAILSGAFPY
                     SLEMIGLKHLPAHTFGVLMSVEPALGAVIGRILLQEKLALLQIVAIACVIAASVGSTL
                     SSRRAAPVSDVA"
     gene            complement(12218..12718)
                     /locus_tag="VNN25_01105"
     CDS             complement(12218..12718)
                     /locus_tag="VNN25_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH90149.1"
                     /translation="MKAIDLMLMEFEQESATTRRLLERVPTEKLGWRPHPKARSLGEL
                     ANHIASSQKRVAAAIQTTPYDLGSGGDGSVPANAADIVTTFDSNVADAKGMLAGMTDA
                     DLMSVWEGQVGGNTVFSAPKIGVIRAILLNHTYHHRGQLSTYLRQLDVTLPSVYGPTA
                     DENPFM"
     gene            complement(12735..13166)
                     /locus_tag="VNN25_01110"
     CDS             complement(12735..13166)
                     /locus_tag="VNN25_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXH90150.1"
                     /translation="MNDASVVVRRVFRASRERLFRALSDPNELIQWFSPEGYRNPSVD
                     ADVKPGGCFRIAMQKLPDGEPFFVSGAYRVVEPPARLAFTWTWEDPKLNVRDTLVTID
                     LLDRGEDTEVVLTHELLPVERRESHSQGWNAMLAKLATTID"
     gene            complement(13186..13518)
                     /locus_tag="VNN25_01115"
     CDS             complement(13186..13518)
                     /locus_tag="VNN25_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006078595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXH90151.1"
                     /translation="MVNSSLHLDRVFHALADSTRRGILAQLARQGQSSVGELAEPHDM
                     SAPAISKHLRVLEEAGLIEREIDGRVHRLSLGSTPLDEATRWIEQTRKFWESQFDSLE
                     RHLQRNPH"
     gene            13720..13872
                     /locus_tag="VNN25_01120"
     CDS             13720..13872
                     /locus_tag="VNN25_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90152.1"
                     /translation="MAKTPAQRGALGRRRKTAGYKAAATKRHRALAQKASATTGKKNE
                     ESPKKP"
     gene            13869..14213
                     /gene="arsC"
                     /locus_tag="VNN25_01125"
     CDS             13869..14213
                     /gene="arsC"
                     /locus_tag="VNN25_01125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746035.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HXH90153.1"
                     /translation="MTDLFLHNPRCSKSRAALALVREAGVELPVREYLQDPLSVAELR
                     RIVKLLGVRPIDIVRRGEPQYKALGLGDATPDDEVLRAMAEHPILIERPIVVRGGRAV
                     VGRPPETVKEIL"
     gene            14329..15384
                     /locus_tag="VNN25_01130"
     CDS             14329..15384
                     /locus_tag="VNN25_01130"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXH90154.1"
                     /translation="MKNIAIGAVLFLAALNVAGQSAIRLPEPSPAATVGQTIGLTDVT
                     INYHRPAVNKRKIWNGLVPYGSLWRTGANENTTISFSTPVRIEGQLLPAGTYALYAIP
                     AASQWTMIFSRFTGDWGVYNYDPSEDVLRVTVTPQTSADSEERLTYRFDDVTNSSAIA
                     SLRWEKVRVPIKIEVDLKATIRASISDTLRGGKHWDPNAWAAAARWELRNGDPDTALT
                     YADHSLALGTTYGGLRTKAAVLDKKGDAKGAAELRDRAASIATEAETIGSGYGVPLGA
                     KRYDESISYLNNYATAHPSSRELWRVYTGLGEVYATKGDPAKAKEYFDKAMAAAHDTA
                     EKTEVWDSINSAAAEAK"
     gene            complement(15515..16504)
                     /locus_tag="VNN25_01135"
     CDS             complement(15515..16504)
                     /locus_tag="VNN25_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH90155.1"
                     /translation="MQKRKLGKSNLEVSALGLGCMGLSFGFGPATEKQEAIALIRAAV
                     ERGVTFFDTAQVYGPYVNEEVVGEALDPFRDQVVIATKFGFAFDAEKKQVGLGSRPEY
                     IKQMTEGSLKRLRVEAIDLYYQHRVDPNVPIEDVAGAVKELIAEGKVKHFGLSEAGVR
                     TIRRAHAVQPVTALQSEYSLFWREPEEEILPTLEELGIGFVPFSPLGKGFLTGKIDET
                     TTFDKTDFRNTVPRFNAENRKANQALVDLVSSIAAKKKVTPAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLGGANVELAPDDLREIESAAAEINVQGARYSEGAQKMIDR"
     gene            16742..17926
                     /locus_tag="VNN25_01140"
     CDS             16742..17926
                     /locus_tag="VNN25_01140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90156.1"
                     /translation="MITILHVITSLTRAGAESQLLNIVCNTTSDELSHIVCYLHPPDD
                     LAADFRSSGIETICLNLPRRRPWLSAPWKLIPILRARRPDFIQTRLLDADLSARFSTL
                     LGPSIPIVNTLHMPTYDAETIRVGGWPVYKMAVLKRLDRWTARATRPLFIAISESVKR
                     SAVRNLGLSPAAVRVIYNSIDQKRLQCDPEEPRRIRREAGIPAGAFVYLTVGRLSPEK
                     GQTVLLRAFKEVAAAFPDAYLVFVGEGPQAGAHETLATELGIRDRVRFLGNRGDVGAC
                     LEMAGAFVFPSLFEGWGAAPIEAMFKGLPCIVTRIEPILELFVDGETGLVVTPDSEAE
                     LASAMMMLSRDPDLRRRLGAAARASAVERFDSRQGMRVWSQFYRELAEMHQYSATSEK
                     SP"
     gene            complement(17904..19040)
                     /locus_tag="VNN25_01145"
     CDS             complement(17904..19040)
                     /locus_tag="VNN25_01145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90157.1"
                     /translation="MRRGSVAAVPPRVTVLLATYNDEPFLGAAIDSVLAQTFSDFELL
                     VVIDACTDRSRDIVESYRDSRVRLLVNETNLGLAASLNRGLAIIASDYVARLDGNDLC
                     FPERLARQVAYLDAHPEVAVVGSQATLIDVAGRRTAEMRRPVTDLGLRWLSIFGSPLI
                     HSSVMFRRAIVWDALGGYDGRHRVGEDFEMWNRLAKEHAVGNLPEKLVAYRDDPYSTT
                     GAERHPERDKYLKRKAPMVYENLRRELRWDDVPRRWGECWVETSDALIPQTADAVRDA
                     VAMIESCAERFASLHPEAAANEEVIVHQADMLALALVKAPVAGRLFSLQLWKVIWHRH
                     RKTALRVLPRFATVLLLGQGPLRFHRVLRRRRADRWRLRATSPR"
     gene            complement(19082..20584)
                     /locus_tag="VNN25_01150"
     CDS             complement(19082..20584)
                     /locus_tag="VNN25_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH90158.1"
                     /translation="MRRLLPISLFLVFAPTLFAQQQPFSLAIDVGDRFTSGTLADVRF
                     TIENISDQDRTNVFLDMFLGNGSAMTKMTPSDPATDCSTLNIGQSRVGCRIPLLAAHT
                     KSEVTATAHYPDAGHYSLRFFSTVEISRDATFYREYPVTTALDGGPGSFRQAILDTNA
                     ECPANIPCRIDFEISEPVPDEGWFTIAPKSPLPEVVAPDIAIDGERQTTISGNTNPKG
                     PEIFLDGRGVDTADGLTILGARATVRGLAIGGFPGNGMLVLRSAMLVEDNYIGIDPAG
                     TIPVPNGLRGIMGDGYGAEIRNNVLSHNTRSGVFLWHNANIHDNRIESNGASGIFLGG
                     ESGFDDSVIVNNIITGNHDFGIAVPHRNLVDVRANSIAHNAGGGIDVGLDGRTLETDD
                     SFVGPVVAPHIESARFDAASGDTIIDGPTPFGFSPRTIYLYANATLDADGFAEGERFL
                     GSPQFAGGRFTLRVHEDLRGLFVDGNTTVTQNDLIITHATSEFGPPVRVE"
     gene            complement(20707..21096)
                     /locus_tag="VNN25_01155"
     CDS             complement(20707..21096)
                     /locus_tag="VNN25_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH90159.1"
                     /translation="MVDEEVPNEDARRALVVDDDAAIRILVSRVLSRRGFLVDAAADG
                     AEAIEDLLEHDYDLITLDLMMPRIDGYGVVKYLQEHRPELFNRLIVMTAFGPHALEKI
                     CPPVVRMIHKPFEIDELVREAEEAIAS"
     gene            21341..21787
                     /locus_tag="VNN25_01160"
     CDS             21341..21787
                     /locus_tag="VNN25_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90160.1"
                     /translation="MFELLLPKSFDNNYRGQKLALWIFGLFALLKSVIGVNSIINGRL
                     VMTGADGIPLDTYPAPAAQSLVALWALLGLAHIIIGLLCVFALIRYRSMVPFLFTLLL
                     LQHLGGRVILEYIPLVRVGAPPAFIVNFIQLSLLILGLALSLWRRR"
     gene            22044..24401
                     /locus_tag="VNN25_01165"
     CDS             22044..24401
                     /locus_tag="VNN25_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH90161.1"
                     /translation="MTDTDLQAELPVLIDAILDYAIILIGKDGAVRSWHTGAVRTMGY
                     EAGEIVGSHFSRFYEPDAIAAGQPQADLEKAAREGRAEDEGWRLKKDGTRFWASSVIT
                     PVRGPGGEITGFASVTRDLTQRREAEERLRQSEETFRLLVASVKDYAIFLLDPTGKIM
                     TWNAGAERIKGYAPSEIIGKHFSAFYPEADIQNGKPAMELEVARETGVFEEEGWRLRK
                     DGTRFWANVVITAVHDESGELRGFAKVTRDLSKRREADERLRQSEEMFRLLVASVKDY
                     AIFLLDPEGHVMTWNAGAERIKGYAPREIIGHHFSDFYSEEDKRNGKPARELEIARES
                     GSVEDEGWRLRKDGTRFWANVVITAIFDERRVLRGFTKVTRDMTERKRAEEVQQAFLE
                     QREARLQAEEERRRAEASVRAAQEANRAKDEFLMTLSHELRTPLTSILGWARLLPTMQ
                     PEDQGFADAVGSINRSAQLQARLIDDVLDVSRIVSGKLRLTVEDVEIDHVLAAAVETV
                     GPSADAKMITIEKELAPQLGLIVADATRLQQVVWNLLTNAVKFTPRSGRITLRARRTS
                     SHLQIAVTDSGAGIDPGFLPYVFEPFRQAENPRTRVHGGLGLGLSIVRYLVEAHGGTV
                     AAESAGRDQGSTFTVTLPLGALASQRPEPITFSALPSVTDLPKKLGGVTILLVDDDVE
                     SRRLVHTVLTRAGAQVTVTASGPEALESIKQRTFDLVITDIAMPHMDGYTLARKIREQ
                     VPNQKIVALSAFPAGRAGMADTPFDVYLFKPIEPVELVDAIAAAV"
     gene            24516..>26521
                     /locus_tag="VNN25_01170"
     CDS             24516..>26521
                     /locus_tag="VNN25_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90162.1"
                     /translation="MFAVVAAVFALTAHAQTLTVYDDALQNGFADYSFGGGTNLNDTP
                     FVHTGTKSASILGHNFNALSFAHAPGNVLTALHTSDTPILRFWVNGGTASGQQFHFSL
                     QLNGAFVGSAAALDTYISGGGVAQNVWRQVTIDLRQAPFNAVDFDRINLQSDEDAAHT
                     DASATYFDDFVLGQPTASAVNPMQIEHDVVVHAITSDRFTWRDSSGQPRVASLAHNDN
                     ETEGGSHGGALREFKYQLSNGQTRTATVTTYGNAGYGGFGYVTMHSAHYAGCNGDDSP
                     LGMGFAGHWQRIFEGKHHAIFRFTQLYPRNCPTSGSIITRYLPVTIDWVFSTGHDNPL
                     WTVTYDIDQIAATGGQPVAANMYWDDSRAPYGELNIDGDGGTDLNGVAWGDFYKFTTT
                     NATGTAITWNSPFDWTQQNTVPFVKEWLDAPLTADNPAKHDATIGIVQTQMIRQQDAG
                     GARDQSVGSDITPYWHKTQSDGVQSAGPGHALPNGDNWPYQACGDNLYADGITTNNAR
                     MTWKTQFGFAGQSTYTINNGAVESSADTAAGYPKKSYSTYIVLGQHSTLPVDAQIAQV
                     ETVQSLTLTAATGTVVTSGPAGVTRGDTVPYAPAGYNHVYGALAFQASGNALDANIQV
                     TPITTFDVSAPGFYYAFNGIAGQNPPLSLVQGVTYHFNINTTAS"
BASE COUNT         4950 a         8178 c         8462 g         4931 t
ORIGIN      
        1 ttcggcattc gcggcctgat gccggcgctg tggctggagc cgattcgcga tctgccgcgg
       61 ttcatgatga tcgcggtcgc cttcggcgtg ttcgtcgtga ccgcggggct cgtgctcaac
      121 atcatcaatc gctggcgcag cggcgacatg cgcgaggcgt tgtacggacc gcgcggtgcc
      181 acgtgggcga tgctgtactg gatcattctg gtgctcctgg cgcgcgcgtt cgtgccggca
      241 acggtgcgca ttccgggcag tgtgatcgcg tacgtcggaa ttggcgcgat cgtcgtcatc
      301 gccggggccg gtccgttgat gaaatggctc ggtcgcgatg gtgagagcag gccgcgtccc
      361 gctgtgcgcg cgacgccttg gtggctgcgc gcgctggagt cgtcggtcga gctggtcgat
      421 acgctgttct cgtttttcgc gaacacgatc tccttcgtcc gcatcgccgc gtttgcggcc
      481 gtacacgccg gcgtgttcat cgccatcttc gcgctggcgg acacgatcgc ggcgatgcgc
      541 ttcggcggcg tgctgtcgat cgtcgttcac gtcgccggca acgtgctggt gattctgctg
      601 gaaggcttga ccgtgtccgt gcaggtcctt cgtctcgagt actacgagtt tttcgggaag
      661 ttcttccgcg gcggcggcga acggtacgcc ccactgacgc tgcggccagg gagtgaacgt
      721 catgcgtaag aaactactgt ggacgatcgc ggcgattgtg ccggtcgtcg ccgcgcttgt
      781 tgtcgttccg ctgttcgccg caccggaagc ggcgcgcgtg gcagctccgg ttccgccgga
      841 ggtggtgcga tggggatttg tcgcggcggc gatcgccgcg ggcctcgccg cactcggagc
      901 ggcgtatgcg gtggcggtcg tgggcgccgc agcgatggga gcgattgcgg agaagccgga
      961 gacggctggc cgcgcgctga tcttcgtcgg tctggccgaa ggcatcgcca tctatggatt
     1021 gatcatcgcc atcatgatcc tcgggcggct tggatgaaac cgttcgtaat cggctcgcct
     1081 gaagacgtcg ttggattcgc gctggcggga atcgacggcg tcatttgcgc gacgcgcgaa
     1141 gaggctgacg ccgcgctcac tcgcgtggac gccggcgtac tggtcatcat gtcggccgac
     1201 tttgcgcgcg cgccatcgcc cggcaacctg atcgtcgccc tcccggcgag gatgtaatgc
     1261 agacgctcgg cagtcccgcc tcgatcattg ccgcgatcca cgaagaagcg gatgcggaag
     1321 ccgagcgcat cgacgccgcg actgcggtcg agttggcgtc gatccgttcg gacgccgcag
     1381 ccgcaaacgt cgggatcgcc gatcgtgaag agcggctgag tgccgcacgg cgaatgaatg
     1441 aagagcgaat ggcaaagcag gagtgggatg ggcggcgcgc cgtcatcgaa cagcgggaga
     1501 aatggattca gcgcgtgtcc gcagcggcgc ggacgaaatg gtcgagcggc ccgccggcca
     1561 cgcgccgcga acagctcaac gcgctcattc gtgaggcggt gtcgcgattt ccgcgcggga
     1621 cgtgcgaagt tgccgtctgc accgccgatc gcactctcgt cgatgtttct gacgacatca
     1681 aagtcaccac cgcaccgatc gccggcggct gcatcgtcac gattggcgac gtctcattcg
     1741 acaacagctt cgaggcgcgg gcgcgccgcc aggagccgga gtggcgcaac gcgctgagcg
     1801 ggatgtacac gcgatgagcg atatcgcggg ccgcatcaca gaaatctccg gctgggttgt
     1861 gcgcgcccgc gcgaacgtac cgctcgcgtt gcgggaagtc gtgctcgtcg ggactgagcg
     1921 gctcatcggc gaagtcatcg ccctcgatcg cgctgaagcg acgattcaag tctacgaaga
     1981 aaccgaaggt gtcgcgccgg gcgatccact attcgcaacc ggcgatccgc tctcgatcga
     2041 gctcgggccg ggccttcttg gtggcatctt cgatggtatg cagcgtccac tgcggagcct
     2101 cgccgatgcc gagggcgact tcattggacg cggcgcgcag atgccctcgc tcgatcgcga
     2161 gaaattgtgg gatttcatcc ccgaagtcga agccggacga gaggtgcatg aaggcgatgt
     2221 gctcgggcga gtgctcgaaa cgcccgtgct cgcgcacccg atcgccgttc ctcccgggat
     2281 tagtggaaag ctcgcctgga ttgcgtcagc gggaccgcgc cggatcatgg acgccatcgc
     2341 gcgactcgat gatggccgcg agctgaccat gttgcagaag tggcgcgcgc gtgtgcgccg
     2401 cccagtacgc gagcaccttg tgccagacgc gccactgatc accggacagc gcgtgctcga
     2461 cacctttttc ccgctggtgc gcggcggaac cgcagccatg ccgggcggct tcggcaccgg
     2521 aaaaaccgtc acacaacatc agctctgtcg ctgggccgaa gcggatgtga tcatcttcgt
     2581 cggatgtggc gagcgcggaa acgagatcac ccagctcatc gacgaactgc cgcgcctcgt
     2641 cgatccccgc acgaagcgtc tgctcgcgga gcgcagcatc ctcatcgcca acacgtcgaa
     2701 catgccggtg tcagcgcggg aggcatcgct ctttaccgga gtcacgatcg gcgagtacta
     2761 ccgcgacctc ggctacgacg tccttctcct cgcggactcg ctttcacgat gggctgaagc
     2821 gttgcgcgaa atctcgggac gactcggcga aatgccagct gaggaaggct atccgccgta
     2881 tctttccagc gcgctcgccg cattcttcga gcgcgccggc agcgtggtca cgctctccgg
     2941 cgggcatgga tcgctgacga tcatcggcgc tatttctccg ccagccggcg acctcactga
     3001 gccggtgacg cggcacgccg cgcgattcgt gcgcacgttc tggacgctcg accgccagct
     3061 cgccggcgca cgcgtctttc ccgccgtgag cacacggtcg tcgtacagcg aagttccgcc
     3121 gggtgtgacg agctggtggt cggagaacgt gtcgccagag tgggccgcga cgcgcgatgg
     3181 cgcgctgcga tttctcgagg aggcatcgct gatcgaggag accgcgcgcc tcgtcggcgt
     3241 cgacagcctc cccgagcgcg agcgatacct gctgcagctc tccagcgcgt ttcaggaggg
     3301 ttttcttcag caggacgcgt atgacgcaag cgatgcgtgc tgcgcgccgg cccggcagat
     3361 gacgttgctg gagacgttca tgcgcgcacg aagcgaaggt ctcgagcaga tcggcaaagg
     3421 agcgccggtc tcgaccgact tcgcggcgct gcagcggatg aagacggagc cgccgtcatg
     3481 accaccgccg gtctggagcg ccgcggtttg gccgagttgc ggggtccgct tgcctttgtc
     3541 gaaggcgtgt caggcattgc ctttcacgaa tttgtcgatc tcgtcggatc ggatggcgag
     3601 acgcgtcccg ggcgcgtttt gtcgacgacg gaaaagggcg ccatcgtcga agtcttcggc
     3661 gtgacggatg gcttgcaact cgacgcatcg cgggttcgct tccacggccg gccgatgcga
     3721 ttcagcgtcg gtctggaact gctcgggcgt gtgttcgatg ggcttggacg tccgcgcgac
     3781 ggattgccgc cgccggcatg tgaggaacgc cgcacgatca acgggtcgcc catcaatccg
     3841 tgccgccgcg cgtatccgcg tgacttcctg gagaccggga tctcttcgat cgacgcgttg
     3901 aacagcatcg tcctcggaca gaagattccg atcttcaccg agagcggact cgcccacgac
     3961 gatctggcga tgcaaattat ccggcaggcg cgcgcgccgg gcgtggaaag atttgcagtg
     4021 gtgttcgcgg cgcttggtct gccacgcgac acggccgagc gttatctcac cggcctgcag
     4081 gccagcggcg cgcgcggacg tctcgtcgca tttctcaacc tcgccgacga tccggccgct
     4141 gagcgtctca tcacgccgcg cctggcattg accgcggccg agtatctcgc ctttgacgct
     4201 ggctatcacg ttcttgtcgt tcttcacgac atcaccaact acggcgaggc gctgcgtgag
     4261 atctctgcgg cccgagggga ggtgccgagc cggaaagggt acccgggtta tctctacagc
     4321 gatctcgcct cgctcttcga acgcgccggg cggatcgccg gccggcccgg atcgatcacg
     4381 cagatcccga tcgtcacgtt gccgtcgggc gatctcacgc atcccattcc tgatctcacc
     4441 ggttacgtca ccgagggaca gatcgtgctc gatcgcgaga ttgcacggcg cggcgtctat
     4501 ccgccggtcg cggttctgtc gtccttgtca cgattgatgt cggatggcat cggcgaaggc
     4561 cgtacacgcg acgatcattc ggacgtggcg aagcaactct acgcggcggt tgcgcacgtg
     4621 cagcgcgcgc gcgggctggc gtcgatcatc ggcgttgagg agttgacgga gatcgaacga
     4681 acgtatctcc gattcggaga acgcttcgaa cgggagttgc tcgcgcaggc cggcgatgaa
     4741 tcgcggacga ttgcgcagac tctggatcgc gcctggagcg tgctgcgtga attgccacgc
     4801 gccgagatga tccgcgttcg cgcgccaatt ctggagaagt acttcgcgtc atgatcgggg
     4861 cggcgacccg atcgcgtctc ttcgagcttc gccgggaccg ttccgcggcg cggcacagcg
     4921 cggagctgct cgatcgaaaa cgcgaagtgc tgctgcggga gatcgcacgc cgcgagcggc
     4981 tgcgcaacga tctccgcgcc gtcgtcgccg cgaagtatgc tgaggcccaa ctgcgccttc
     5041 gcgtcgcccg cgtggagctc ggcatgcgcg gcgttgaagc cgtggcactc gctcaaccgg
     5101 tccggcagat cattgctcgc cgcgacacct cggtcatggg cgtgcgcatc ccgcaactcc
     5161 gcgcaacggt tgaaccatac cgcgcgtttt acggcgccgc cgcaaccacg gaatcgctcg
     5221 acgagtgcgg cgcagccttc accgatctcg ttccgcatct cgtagcgctt gcgcaggaag
     5281 agatcgccgt catgcgcctc tcggcggcca tgcgaaagac gacgaaactc gtcaacgcgc
     5341 tgcagagagt cgttctccct cgaatcgaaa aggaaatccg cgccatcgtc gacggcatcg
     5401 aagaagacga gcgcgatgag tcagtgaggc ggcgggtagg acgcgccgag agattgtgaa
     5461 gaaatcgttg acgacatcgt ccggcggatc cgagtaccaa gcaaccggtc tcgtgcagta
     5521 agtcccgcgc atagttccag agccgatcat tgccgcctgg atcatcacgt cggtccatct
     5581 tcgtccgcag gaaacgggag cgcttgggcg gggggtgttc gcaaggtcgc ggagacgatg
     5641 ggccagacct tcgggccggc attggggaca cgccttaagc cgccctcgac gcgctattcg
     5701 ccgcgctcca ctgctcgtat cgcgattttc cattccgagg aggtcggcgg gcgtttttct
     5761 ccacctcacg tggattcctc ggccatcttg acagtgccga gggatcctcg ggcgcgttat
     5821 ggcccagaag aagacgaccg ccggaacgtc gcggtcgaga atcgtcaagc agccggtggc
     5881 tgttgcttcc gcgcgcaaac cgtcgcggac cgccgccagc ccagccgcac tgctgtccga
     5941 ggtgcgggaa ctgatccttc agacgcggaa aacggtcgcg cagggcgtca actccgcgct
     6001 tgtgttgctg tactggcaga tcggccagcg catccgaatc gacatcctga aggagaagcg
     6061 ggcgggttac ggcgaacaga ttttccactc actgagtgga aaattgactg cggagttcgg
     6121 cggcggatac acggagcgga atctggccaa catggttcgc tgtgccgagg tgtttcccga
     6181 caggaacatt ttgcacacac tgtgtgcaaa attgagttgg tcgcatttcc gccgcctcat
     6241 ctatgtcgat gaccccttga agcgagagtt ctatgcagag atgtgccgcg tcgagcaatg
     6301 gagcgttcgg acactccatg aaaggatcca atcgatgctc ttcgagcgaa cggcgctctc
     6361 gaaaaagccg gatagtctga tcgccgcgga gttgaagatg ctccgcgacg aggacaaact
     6421 ctcgcccgat ctcgtcttcc gcgacccgta tgtgttggac ttcctcgggt tgaaggacac
     6481 tttcggtgag aacgatctgg aggcggccat tctgcgcgag atcgaggcct tcatccttga
     6541 gatcggcgtc ggcttctgtt tcgtcgaacg acagaagcgg atgcagatcg acggacgcga
     6601 ctactacctc gacctcctct tctaccaccg caagctgcgg cgactggtgg ccgtcgagct
     6661 gaaagtcgga gacttcgaag ccgcagacaa ggggcagatg gagctctatc tgaactggct
     6721 gaagcgacac gaacgcgagc gcgacgagga cgagccgttg ggaatgatcc tctgcgcagg
     6781 caagtcgcaa gaacacgtcg agcttctcga actgcagaag agcggcatcc atctggccag
     6841 ttacttgacg actctcctgc cttggaagca gctcgagacg aagttgcgcg aggcggtgaa
     6901 actggcgcgc cagcggcttc aggaaccggc attgggggtc gcgcagggtc gcccgtcgtg
     6961 agtgagaccg gcaatgagtg gaggtccggc ctgatttcgg gtggttcaac agcagtggct
     7021 ggcgccaccg cagtccaaag cgcttcgcgc gaaataagat gagcactggt ggtcgagatt
     7081 gaccttgtcg cggacgcgat gtgacctgcg ttacgcgccc cgccagcctg ggggcctatg
     7141 ttcagagaga ttcaatgaca tggagatctc gtggtgcgct cctcgctcag actctcgctc
     7201 ccgtttgttt tcgtagttgc tgtcgcctca ccgatgttcg cggtcaccta cgtcgttccg
     7261 gcggatcgcg tcgaggttga gcgatcgaat gccatcatcg tcggccgcgt gcttcgatcg
     7321 cacgtcgaaa gatcacccca gttcgggatc gagaccgtca cggacgtcgt tctcgaagaa
     7381 acggtcaaag gaagtgtcga aagcttattt cagatccacg agcctggcgg agtgctggac
     7441 gaccaggcgt atgtgattcc aggagtgccg gcattcatcg tgggcgaccg cgtattgctt
     7501 ttcctctacc agcgtgagaa cggcgagtac accgtcaacg acttcgaact tggctcgttc
     7561 catttcacga aggacgccgc tgggcgggag ctggtgattc gcaacgagtc cgaggtgaac
     7621 ggttgggacc tcgacggcac gccgcacaag gagccgcacc gtgccgcgca gcaattcatc
     7681 aactacattc ggggcatcgt acgcggtgag gaagtcggag aggactatgt cgttgcgacg
     7741 ctgcctctgg ccggtgtaac agaaacggtg aaagcggaac ccggctctct tcatccgatc
     7801 accacggcgg cgttcacggc ggcctcctat accgtcgatc tctccgccgg gttgggggcg
     7861 cggtggaaca tcttcccgag caccgtgagc tggaatcggg ggaattcgga gaccggcgtt
     7921 ctcggcaatg gcgcgtcgca gatcgccacc gccttcgcct cctggaatgg tggcggtgcc
     7981 cactatgtcc tggcgagcgc aaaccccaat cccaacggca tcatggacgc gctcgacggt
     8041 atcaacaaca tcgttttcga gaaaaacctc actggcgccg gtctccaacc ttacagttgc
     8101 accaagggcg gcgctcttgg catgggcggt atgcactctg ctctgttcgg tggtggcacg
     8161 cacgtatttc acggcgagac attcggcacc accgtggagg tcgacgtcag catgaatcag
     8221 ggcctcgggg cctgcaccct ggcgcaactt tcgtcagagc agttcaatac gacggtcacg
     8281 cacgaagtcg ggcacacgct cgggttccgt cactcggatc agaaccgtac tcaaaatgcc
     8341 ttgtgcacga ctgaccccag tcttgattgt agcaacgcgg cgctcatgga tcacctcctc
     8401 ctcgtcggtc tcaacggcca gcttcaaccc tgggacagca gcgccatcag catcgtttac
     8461 ggcaacggcc cggcgtgcac gccgccttcg atcgcccagc agccgggcgg ttcgacgatc
     8521 accagcggca actccgcgtt tctctcggtg accgcaacgg gcactcctcc gctcacgtac
     8581 cagtggttcg ccggttcgag tggcaacacc tccacgccgg taaacggcgc cggcgcgacg
     8641 atcagcgtcg gccccgcggt cacgacctct tactgggtcc gcgtcacggg ccagtgcgcg
     8701 cctgccgccg acagcgccgc cgctaccatt accgtgaacc cggccaactg tccgaccgtt
     8761 gttctcggga cgccgcaggc gattcctgtg aatggcggct ttcagctctc gatcaatacg
     8821 agcggcggca attcgtttac ctacctgtgg tttctgggga cgagccccgg cctcggctca
     8881 cagatcggtg caggcaatcc tctgctcgtg aatccggcgc agacggccag ctactggtgc
     8941 cgcgtcacga acaactgcag caatacagca gacagcgccg tcgtgacggt gacgatcgtc
     9001 ccgtgctcag cgccgcagat cgtcaaccag ccgcagaatc agactgccct catcgggacc
     9061 actgcttccc tgacggttgg cgccaacggt accgctccga cgatcacatg gttccagggg
     9121 ccgagcggaa acacgtcgac tccagtcggt tcggggaaga cgatcaccag ccccgttctg
     9181 acgcagacga cgcagttctg ggctcgcatc accaattcgt gcggctcgat cgatagcaat
     9241 gccggtacga tcactgtgga ggtcgcccgt catcgcgcgg cgcgccgata agttgcgaag
     9301 tgaattggca gaacgcgttt cacgcggaga cgcggagcac gcagagagaa cctccgcacc
     9361 tccgagcgat ggtcttggcg cggtgtcctt cgctgtcagt gctgatccgc agccgtcccg
     9421 tccgaccatt tcccgaccgg aaccatgaac acggtcatcc ggccaagatc gtccgaggcg
     9481 ctcaaaagtg gggcgccggg cagacctgcg ccccaggttg caggatctgt cagtggaagg
     9541 aagaacatca ggtgggggtg ccagtgtccg gcggcgtcgt tcaggtaacc ttgctttgac
     9601 atcatgtagc acatcgcgcc cagctccggc ggcggcaatt ccttcttgtc gatcgcggcc
     9661 ttgatgtcct tgaccatctc aactttggag cgtgtcgcca ggatcaccgt ggttttcctt
     9721 accgttagtg ggagatagga ccgcgaggcc accgcgttga agcagttcgg agcccgcaac
     9781 ttagggttcc agaagtctgg atggtcgagt ggggaggccc acgatcgttc caccaggcag
     9841 acgaaaccgt tcttgccttt gacggcggtc tcgtaaccat gttctccgag gaccatgacc
     9901 tcggcatccc tcgagatgga gtcgggagca gcgctccgcg ccagcgcgat ctcggcatct
     9961 cgctccatca tgtattggtc aagcggcgcc atgctcggat acggtgtttt gacgtcctgg
    10021 gcttgcgcgg ccaacgcggc tgccagcacg atgacgaccg cgaagcttgt gaacgcaatc
    10081 gtcctggcgt ttcgcttcat tgatcactcc tctttcgaat gtgcaccgtg ccggccagca
    10141 tgaacgcgac ggcgctgtcc tggagcgcct gaaacccgcg ggagacctcg ctgacggtat
    10201 gcgtgttgat ggcgagggga atgtgaacga ggaagaacca gctgaagagc atgactcccg
    10261 aaagcatcgc cgccatgcgc gccgtttgcg gcacaacgac gccgatccct ccggcgatca
    10321 gcgcgacggc tgcaaagtag gtccagaaca tgtgccccgg aatccacggc ggaatcagac
    10381 ccgcaacgta cttggcgtac ggaaagtgta aagcgccgca gtaagccaga aatgctccga
    10441 gcgcccacgg tacaaagtgc gttgcctttt cgaaaacgcg gtcggccgag ctggccgatg
    10501 atccctcgcg tggatacaca acgaggctca caccgccgag gatcgccagg atcatcaagg
    10561 gatcgaccca tacgtatccc atcgaggcgt gtgggatcgc tacgcgcaag ctgaatccaa
    10621 agaacaacac gagcagtagc gtcgcgaggg cgatggcggc tggcaagacc gttttcctga
    10681 cgagtaagcc tgcgccgatg agaacgagga gcacgccgaa caggtaggcc agccacagcg
    10741 gcgggagatg agcgggattc accggcacga ggttcaccca gcgcgccgtc acgagttggt
    10801 agatccccga cgcgacgacc gtcagagcga agaggagccg gccggtcttc agcagcagcg
    10861 atgatgtttc aggagaatcg agctcgctca ttgccgcgaa aagctagcat gacgacggtc
    10921 tcgcagtgag gtccaaaacg gtgacgcttg caggggtgtc ggttacgaca cttgctcaaa
    10981 gctgtgacct ttttccggtc tgatccgtct gattgatggc agcgtcaggc gacgtcgctg
    11041 acgggagctg cgcgccggct cgatagcgtg ctgcccaccg aggctgcgat gacgcacgca
    11101 atcgcgacga tttgcagtag ggcgagtttt tcctgtagca ggatccgccc gatcaccgcg
    11161 ccgagcgccg gctcgacgct catcaggacg ccgaatgtgt gcgccggcag gtgcttcagg
    11221 ccgatcattt ccaacgagta cgggaacgcg cctgagagga tggctacggc gattccgatc
    11281 ggcaggatcg cgggattcag gagcggtttg ccaacgacgg cgacaccgaa cggcagcacg
    11341 aggagcgcgc cggtggccat gcccaaggcg gtggcggttc cgccgtgaac cgaggcgccg
    11401 gcgcgctggc cgacgaggat gtaagcgccc cagcaggcac cggctaggag cgcccacagc
    11461 acaccatgaa tgtcgactga tggcgacccg cgaaagatcg ggaggatgag aacgatccct
    11521 gcggccgcca gcgcagccca gacgaaatcg atgccgcggc gcgacgcgat caccgcaacc
    11581 gccagcggcc cggtgaactc gactgccacg ccgattccga gcggtatggt cttgagcgcg
    11641 atgtaaaagg agaggttcat tccgcccatg gccatgccgt agatcgcgat ggtccgaagc
    11701 tcggcgcgag tgagcttctg ccgccacggg cgccagatgg cgcagaggat cagcgcgccg
    11761 aagcccaatc gcagcgtggt ggttccgacc ggtccgacga gcggaaagag ttgtttggca
    11821 agcgaggcgc cggtctggaa ggagaccatg gccacgagca cggccaacac cgcgaggagc
    11881 gtgcggctca tgtcgtgcgt ggcttcggga agtgcatgtc gatcgcgagg acgatcgtgt
    11941 tccgttccat acgtaggacg atacacgaag catcgagtcc gctcgtggtg cttacaccgc
    12001 gcgagatgtt ccggagtgca ggcgtctcgc ctgcggccac cggggcgtcc cgcctcggtg
    12061 gttggattcg gcctgatagc cccaaagttg aaactgggct tgatctgaga aacaggccct
    12121 ggctatgtcg ctccaacacc cgactcccaa acttcgaccc ccgccctgac taccctcggg
    12181 cgaggacgcc cgagggtccg cggtcgagac gaccgcacta catgaacggg ttctcatccg
    12241 ccgtcgggcc gtaaaccgac ggtagggtca cgtcgagctg gcgcaggtag gtgctcaact
    12301 gtccgcggtg gtgataggtg tgattgagca gaatggcgcg gatgactccg atctttggtg
    12361 cgctgaagac cgtgttgccg ccgacctggc cttcccatac cgacatgaga tcggcgtcgg
    12421 tcatccctgc cagcattccc ttcgcatcgg cgacattgga gtcaaacgtc gtgacgatgt
    12481 ccgcggcgtt cgccgggacc gagccgtcgc cgccggagcc gaggtcatac ggggtcgttt
    12541 gaatggccgc ggcaactcgc ttctgggagc tggcgatgtg attggcgagc tcgccgaggg
    12601 agcgggcctt ggggtggggc cgccagccga gcttttccgt tggtacgcgc tcgagcaagc
    12661 ggcgtgtcgt cgctgactcc tgttcgaact ccatcaacat cagatcaatt gctttcatgg
    12721 tttcgtctcc ttattcaatc aatggtggtg gcaagtttcg cgagcatggc attccagccc
    12781 tgcgaatggg attcgcgtcg ttcgaccggc aacagctcat gggtgagcac gacctccgtg
    12841 tcctccccac gatcgaggag atcgatggtg accagagtgt cgcgaacgtt taacttcggg
    12901 tcttcccagg tccaggtgaa cgcgaggcga gccggtggct ccacgacgcg gtatgcgccg
    12961 ctcacgaaga acggctctcc atctggtagc ttctgcatgg cgattcgaaa acatccgccc
    13021 ggtttcacat cggcatcgac cgaaggattt cgatagccct ccggcgagaa ccactggatc
    13081 agctcattgg ggtcgctcaa ggcgcggaag agccgttccc tcgaggcgcg gaacactctg
    13141 cggacgacaa cagatgcgtc attcattgaa cactcttcct tccgatcaat ggggattccg
    13201 ctggaggtgc cgctccagcg agtcgaattg cgattcccag aatttccgcg tctgctcgat
    13261 ccaccgcgtc gcttcatcga gcggcgtcga gccaaggctg aggcgatgga cgcggccatc
    13321 gatctctcgc tcgatcaggc cggcctcctc cagtacacga aggtgcttcg agatggcggg
    13381 cgctgacatg tcatgcggct cggcaagctc tccaaccgac gactgaccct gtcgagcgag
    13441 ctgcgccagg atcccgcgcc gcgtcgaatc ggccagcgca tgaaagacgc ggtctagatg
    13501 cagagaactg ttaaccatct ggttaatgat agcggtgagg agatcgggag tcaagagggt
    13561 tcttcgggag tgcatcgggc gacaacctca gtgacctctg tgtctctgtg gttcaaatcg
    13621 attgtggatt ttccacgggc gcgattgcgc taccatctaa cggtggcgcc aggcctgtga
    13681 gtatggagcg ccagccctcg acgattcgca ggagtccgca tggcaaagac gccagcacaa
    13741 aggggagcgc tcggccgtcg tcgcaagacc gccggttata aagcagccgc taccaaacgg
    13801 catcgcgccc tcgcgcagaa ggcttctgcg acgactggca agaagaatga ggaatcgccg
    13861 aagaagccat gactgatctc ttcctccaca accctcgctg cagcaagagc cgcgctgcgt
    13921 tggcactcgt tcgcgaggct ggcgtcgagc tgccggtccg cgagtatctg caggatccgc
    13981 tgtccgtcgc cgaactgcgc cggatcgtga agctgcttgg cgttcgtccg atcgacatcg
    14041 tgcgccgcgg cgagccgcag tacaaagccc tcggcctcgg cgacgccacg ccggacgacg
    14101 aggtgctgcg cgcgatggcc gagcatccga tcctcatcga gcggccgatc gtcgtccggg
    14161 gtgggcgggc ggtggtgggg cggccgccgg aaaccgtgaa agagatcctt taacggacga
    14221 cgcgtctgcg agtggcgcgg agcggcgtct cgacccgtct cgccggttcg ctgcacgtta
    14281 catgatcgat acaggaaggg ccttcgcgct cgctctaaga ttgcgggcat gaagaacatt
    14341 gcgatcggag ccgtcctctt tcttgccgcg ctgaacgtcg ccgggcagtc ggcgatcaga
    14401 ctgccggagc cgagtcccgc tgccaccgtt ggccagacga tcggtctcac cgacgtcacc
    14461 atcaactatc accgtcccgc ggtgaacaaa cgaaagatct ggaacggcct cgtgccgtac
    14521 ggttcactgt ggcgcaccgg agcgaatgag aacacgacta tctcgttctc cacgccggtg
    14581 aggatcgaag ggcagttgct gccagccgga acgtacgccc tgtatgcaat tccggcggcg
    14641 tcgcagtgga cgatgatctt cagcaggttc accggcgact ggggtgtcta caactacgac
    14701 ccgtccgagg atgtcctgcg cgtgacggtg acgccgcaga cttcggccga ctccgaggag
    14761 cggctcacct atcggttcga cgacgtgacc aacagctccg ccatcgcctc actgcggtgg
    14821 gagaaggtgc gtgttccgat caagatcgag gtcgacctca aggccaccat ccgggcctcg
    14881 atcagcgaca cgctacgcgg cgggaagcat tgggatccga atgcgtgggc ggccgcggcg
    14941 cgctgggaac tgcgcaacgg cgatcctgac accgcgttga cctacgccga tcattcgctc
    15001 gccctgggta cgacatacgg cggcctgcgt acgaaggcgg cggtgctgga caagaaaggg
    15061 gatgcgaaag gagccgcgga gctgcgcgac cgcgctgcgt cgatcgccac cgaggcggag
    15121 acgatcgggt cgggatacgg cgtcccgctg ggcgcgaaga ggtacgacga gtcgatcagc
    15181 tacctgaaca actacgcaac cgcgcacccc agcagccggg agctttggcg cgtctacacc
    15241 ggcctcggcg aggtgtacgc gacgaagggc gatcctgcaa aggcgaagga gtacttcgac
    15301 aaggccatgg ccgccgcgca cgacacagcc gagaaaacgg aggtatggga ctcgatcaat
    15361 tccgccgcgg cggaggcgaa gtgagggatt ggcagtccct tcggacggct gatcgaatcg
    15421 cgcatttggg acgtcatctc tgtgtcctca gggtctctgt ggttaaaggc ttttgacacc
    15481 acagagacac aaagggtgca gagaactcgc gagatcaccg atcgatcatc ttctgcgcgc
    15541 cctcggagta ccttgcacct tgcacgttga tctctgcggc ggcgctctcg atctcgcgga
    15601 gatcgtccgg tgctagctcg acgttcgcgc cgccgaggtt ttcttcgagg cggtggagct
    15661 tggtggtgcc ggggatggga acgatccacg gcttctgcgc gagcagccag gcgagcgcga
    15721 tctgcgccgg cgtcaccttc ttcttcgccg cgatcgaact caccagatcg accagcgcct
    15781 gattcgcttt ccggttctcg gcattgaagc gcggaacggt gttgcggaag tcggtcttgt
    15841 cgaacgtcgt cgtctcgtcg atcttgccgg tcaggaatcc cttgcccaat gggctgaagg
    15901 gaacgaagcc gattccaagc tcttcgagag taggcaggat ctcctcttcc ggctctctcc
    15961 agaacagcga gtactcgctc tggagcgccg tcaccggctg caccgcatgc gcacgccgga
    16021 tcgtccgcac gcccgcctcg gatagcccga agtgcttcac ttttccctcg gcgatgagct
    16081 ctttgaccgc gccggcgaca tcctcgatcg gcacgttggg atcgacgcga tgctggtagt
    16141 agagatcgat cgcctcaact cgcagccgct tcagcgagcc ctcggtcatc tgcttgatgt
    16201 actcgggccg gctgcccagt ccaacctgct tcttctcggc atcgaaggcg aaaccgaact
    16261 ttgtagcgat gacgacctgg tcgcggaacg ggtcgagcgc ttcaccgacc acctcttcat
    16321 tgacgtaagg gccgtaaacc tgcgccgtgt cgaagaacgt cacgccgcgc tcgacggccg
    16381 cgcggatcag agcgatcgcc tcctgcttct ccgtagccgg gccgaagccg aaactcaacc
    16441 ccatgcaacc gagtccgagg gcggagactt cgaggtttga tttgccgagt ttgcgtttct
    16501 gcatgggatt ctcctttgtg tctgagaacg atccgcagat gacgcagatg atacgcagat
    16561 aagacccgtt ctgagtctgc gttcaatctg cgtcatctgc ggataagcct ttgacgccga
    16621 aatcggtaaa acgtctgcgt tgatcggacg gccgctttcg cactggattg tgtaacgatt
    16681 caggcgcgtg atgtgtcgta ggatcgtttc caccgcgatc cggccattct ctgactttct
    16741 gatgatcaca attctgcacg tcatcacctc gctcacgcgc gcaggggccg agagtcagct
    16801 cttgaatatc gtgtgcaaca ccacgagtga cgagctctcc cacatcgtct gctaccttca
    16861 tccacccgac gacctggccg ccgactttcg gagctccggc atcgagacga tctgcctcaa
    16921 ccttcctcgc aggcggccct ggctgtctgc gccgtggaag ctcatcccca ttctccgtgc
    16981 gcgccgccct gatttcattc agacccggct tctcgatgct gacctgagcg cacgtttctc
    17041 cacactcctc ggtccctcga ttcctatcgt caatacgttg cacatgccca cctacgatgc
    17101 ggagacgatt cgtgtgggag ggtggccggt gtacaagatg gccgtgctca agcggctgga
    17161 ccggtggaca gcgcgcgcca cgcgtccgct gttcatcgcg atctcggaga gcgtcaaacg
    17221 atcagcagta cggaacctcg gtctttcacc ggcggccgta cgcgtgatct acaactcgat
    17281 cgatcagaag aggcttcagt gcgatcccga agagcctcgc cgcatccgaa gagaggctgg
    17341 aatcccggcc ggagcattcg tctatctgac ggtagggaga ctgtcgccgg agaagggcca
    17401 gacggtgctg ttgcgcgcat ttaaagaagt ggcagcggcg ttcccggatg cgtacctggt
    17461 tttcgtcggc gaagggccgc aggccggtgc tcacgaaacg ctcgcgaccg agttgggcat
    17521 tcgcgaccgc gtacgctttc tgggcaatcg tggcgacgtg ggcgcatgcc tggagatggc
    17581 cggcgcgttc gtcttcccgt cgctctttga aggatggggc gcagcgccga tcgaggcaat
    17641 gttcaaggga ctgccatgca tcgtgacgcg catcgagccg atccttgaac tgttcgtgga
    17701 cggcgagacg ggtctcgttg tcacgcctga ctccgaggcc gagttggcgt ctgcgatgat
    17761 gatgctatct cgcgatccgg atctgcgccg ccgcctcggc gcggccgccc gcgcgtcagc
    17821 cgtcgaacgg ttcgacagcc gtcaaggaat gagagtgtgg tcgcagttct accgcgagtt
    17881 ggcggagatg catcaatact cggctacctc ggagaagtcg ccctaagccg ccagcgatcg
    17941 gcgcggcgcc gtctaagtac gcgatggaaa cggagagggc cctgacccaa gaggagaacg
    18001 gtcgcgaatc gggggagcac gcgcagcgcc gtcttccggt gacggtgcca aatcaccttc
    18061 caaagctgca gcgagaacag ccggccggct accggcgcct tgactagcgc gagagcgagc
    18121 atgtccgctt gatgcacgat gacttcctca ttcgcggcgg cctcgggatg cagtgaggcg
    18181 aagcgttccg cgcaggactc gatcatcgct acggcgtcgc ggacggcgtc ggccgtctgc
    18241 gggatcagtg catcgctggt ctcgacccag cactcccccc agcggcgggg aacatcgtcc
    18301 cagcgcagct cccggcgcag gttctcgtaa accatcggcg ctttgcgctt caggtatttg
    18361 tcgcgctcgg ggtgtcgttc ggcaccggtt gtcgagtatg ggtcgtcgcg gtaggccacg
    18421 agcttctcgg ggaggttgcc cacggcgtgc tccttcgcca ggcgattcca catttcgaag
    18481 tcctcgccga cacgatgtcg cccgtcgtac ccgccgagcg cgtcccacac gatggcgcgg
    18541 cggaacatca cggaactgtg gatgagagga ctgccgaaga tgctgagcca gcgcaatccg
    18601 aggtcggtga ccggccggcg catctcggcg gttcttcgtc ccgcgacatc gatgagcgtc
    18661 gcctgcgacc cgacgacggc gacctcggga tgcgcatcga gatatgccac ctgccgggcc
    18721 agcctctccg ggaaacagag gtcgttgcca tcgagtcggg ccacatagtc gctggcgatg
    18781 atcgccagcc cgcggttgag cgaggccgcg agaccgaggt tcgtctcatt cacgaggaga
    18841 cgaacgcgcg agtcgcgata gctctcgacg atatcgcgcg aacgatcggt gcaagcatcg
    18901 atgacgacga gcagctcgaa atcagagaag gtctgcgcca gaacggagtc gatcgccgcg
    18961 ccgagaaacg gctcgtcgtt gtacgtcgcc aacagaacgg tcacgcgcgg cggcacagcc
    19021 gctacgctac cgcgacgcac acgcgcgggc aatcgcggaa gagccttgcg atagagaatc
    19081 gctactcgac gcgcactgga ggtccgaatt ccgaggtggc atgggtgatg atcagatcgt
    19141 tctgggttac ggtcgtgttg ccgtcgacga atagtccgcg cagatcctcg tgtacgcgca
    19201 gcgtgaagcg gccgcctgcg aattgcggtg atccgaggaa gcgctctcct tcggcaaaac
    19261 catcggcatc gagagtcgcg ttcgcataga gatagatcgt ccgcggagag aagccgaacg
    19321 gcgtaggacc gtcgatgatc gtgtcgccgc tcgccgcgtc gaaccgcgcc gattcgatat
    19381 gcggcgcaac gacgggaccc acgaatgaat cgtcggtctc cagtgtccgc ccgtcgagac
    19441 cgacgtcgat tccacccccg gcgttgtggg caatcgagtt tgcgcgtacg tcgacgagat
    19501 tccgatgcgg caccgcgatg ccgaagtcgt gattgcccgt gatgatgttg ttcacgatga
    19561 cgctgtcgtc aaagccgctt tcgccgccga gaaagatgcc gctggcgccg ttcgattcga
    19621 tgcggttgtc gtggatgttc gcgttgtgcc agagaaagac tccgctgcgc gtgttgtgac
    19681 tgagaacgtt gttgcggatt tcggcgccgt aaccatcgcc catgatgccg cgcagtccgt
    19741 tcggaactgg aatagtaccg gcgggatcga tgccgatgta gttgtcctcg accagcatcg
    19801 ccgaccggag gacgagcatg ccattgccgg gaaagccacc gatggccagg ccgcgcaccg
    19861 ttgctctggc gccgagtatg gtcaggccgt cggcggtgtc gacaccgcgg ccgtcgagga
    19921 agatctccgg acctttcggg ttcgtgttgc cgctgatggt ggtctgcctc tctccgtcga
    19981 tggcgatatc cggcgccacc acttccggta acggcgattt cggtgcgatc gtgaaccacc
    20041 cctcgtcggg taccggctcg ctgatctcaa agtcgatccg gcacgggatg tttgccgggc
    20101 actcagcgtt cgtgtcgaga atggcttgcc ggaacgaacc cgggccgccg tccagggccg
    20161 tagtcacggg atactctcga taaaacgtag cgtcgcgaga gatctcaacg gtcgagaaga
    20221 acctgagtga gtagtgccct gcatcgggat aatgagcggt ggctgtgacc tcactcttcg
    20281 tgtgtgcggc gagcagggga atccggcagc caacgcgcga ttgtccgata ttgagcgtcg
    20341 aacagtcggt ggccggatcg gatggcgtca tcttcgtcat cgcggacccg ttgccgagga
    20401 acatgtcgag gaagacgttc gtgcggtcct ggtcggagat gttctcgatc gtgaagcgaa
    20461 cgtcagcgag ggtgccggac gtaaagcgat cgccaacatc gatggcgagt gagaacggct
    20521 gctgctgggc gaagagggta ggggcgaaca cgagaaagag ggaaatgggg agtagtcgtc
    20581 gcatgactca ttcgacagta cgcctcccga tctcattctg tctacgtggg ttagcggcct
    20641 cgacaggcag acgtcacgcg cgaagaggcg aacgcgcgaa gagggagaac gcgtctaacg
    20701 gactggttat gaagcgatcg cttcctccgc ttcgcgcacc agctcgtcga tctcgaatgg
    20761 tttgtgaatc atgcgcacga ccggcgggca gatcttctcc agagcgtgcg gcccgaacgc
    20821 agtcatcacg atcagacggt tgaacagctc cggtctgtgc tcctggagat acttcacgac
    20881 gccgtatccg tcgatgcgcg gcatcatcag gtcgagcgtg atcaggtcgt aatcgtgctc
    20941 cagcagatcc tcgatcgcct cggcgccatc agcagcggcg tccaccagaa agccgcgccg
    21001 gctgaggacg cgcgacacga gaattcggat tgcagcgtcg tcgtcgacga ccagcgcgcg
    21061 ccgcgcgtct tcgttcggta cttcttcgtc aaccacaccc ttcaccttct gggttattgg
    21121 gaccaagcaa cggtgaagcc aagtaggagg ctgttgtcgg gagtcgggtg tcggcgaacg
    21181 cggacagggt ggctttcact catcaagggt gccagcagtc gagtgtcgat cccccagcta
    21241 gcgaggtcgt gcgtacctta tctccgaccc ccgaccagct atccgttttc aatcggaccg
    21301 ccgaaactct ccatctcacg caacgtacct tcttcccatc atgtttgagc tgctgctgcc
    21361 gaagtccttc gacaacaact atcgcgggca gaaactcgcg ctctggatct tcggcctgtt
    21421 cgcgttgctg aagtccgtca tcggcgtcaa ctccatcatc aacgggcgcc ttgtgatgac
    21481 cggagccgac ggaatccctc tcgacactta cccggccccc gccgcccagt cgttagtcgc
    21541 actctgggct ctcctcggac tcgcgcacat catcatcggt ctgctgtgcg tattcgccct
    21601 gattcgctac cgcagcatgg ttcccttcct gttcacgctg ctcctgctgc agcacctcgg
    21661 cggacgagtc atcctcgaat acatcccgct cgtcagggtt ggagccccac cggcattcat
    21721 tgtcaacttc atccaactct cattgctgat cctcggcctg gccctatcgc tgtggcggcg
    21781 acggtaaacg aatgcccctg gggtctttcg cgacgaagtc gctgtggact gcggtggcgc
    21841 agccaccgct ttgaatcacg cgttcgaaag cggcagctgg cgctgccgca gtccaaagcg
    21901 cttcgcgcga aaacgatcga cacgccgatc actcgcacgg tcgagccaga ggaatcagcc
    21961 cctctccccc gtggcgcgct ctttgattac attgatcctg ggctggggac tagggatagg
    22021 cttgcctggc gaacgactga actatgacgg atacggatct gcaggctgag cttcctgttc
    22081 tcatcgatgc gattcttgac tacgccatca tcctgatcgg taaggacggc gccgttcgct
    22141 cgtggcatac gggagcggtg cggacgatgg gatatgaggc gggggagatc gtcggcagtc
    22201 acttctcgcg cttttacgag cccgacgcca tcgcagcggg gcagcctcag gcggatctgg
    22261 agaaggcggc gagggaaggg cgcgccgagg atgagggctg gcggctgaag aaggacggta
    22321 cacgcttctg ggcgagctca gtcatcacac cggtccgcgg tccgggcggc gagatcaccg
    22381 gatttgcgag cgtcacgcgg gaccttacgc aacgtcgcga ggcggaagag cgcctgcggc
    22441 agagcgagga gaccttccgg ttgctcgttg cctcagtgaa ggactacgca atctttcttc
    22501 tcgacccgac cggcaagatc atgacgtgga acgcgggtgc cgagcgcatc aagggctatg
    22561 cgccgagcga gatcattggc aaacacttct cggccttcta tcccgaggcg gacattcaga
    22621 acggcaagcc ggcgatggag ctggaggttg cgcgggagac gggcgtcttc gaggaagaag
    22681 ggtggcggtt gcgcaaggat ggcacgcgct tctgggcgaa tgtcgtgatc accgccgtac
    22741 acgacgagag tggcgagctc cgcggcttcg ccaaggtgac gcgggacctg tcgaagcggc
    22801 gcgaagccga cgagcgactc cgccagagcg aggagatgtt tcgccttctc gtggcctcgg
    22861 tgaaggatta cgcaattttc cttctcgatc ccgagggcca cgtgatgacg tggaacgccg
    22921 gcgccgagcg gatcaaaggg tatgcgccgc gcgagatcat cggccatcac ttctcggact
    22981 tttactccga ggaggacaag cgcaacggga agccggcgcg tgagctcgag atcgcgcgcg
    23041 agagcggcag tgtcgaggat gaaggctggc gcctgcgcaa ggacggcact cgcttctggg
    23101 cgaatgtcgt catcaccgcg atcttcgacg agcgccgggt gcttcgcggt ttcacgaaag
    23161 tcacgcgcga catgacggag cggaagcgtg cggaggaagt gcagcaggcc tttctggagc
    23221 agcgtgaggc acgactgcag gcagaagagg aacgccgccg cgccgaggcg tccgttcgcg
    23281 cggcgcagga agcgaatcgc gccaaagacg agttcctgat gacgctttcg cacgagctgc
    23341 gcacgccgct gacttccatc cttggatggg cgcgtttact tccgaccatg caacccgagg
    23401 atcagggatt cgcggacgcg gtgggatcga tcaatcgcag cgcccagctc caggcccggc
    23461 tcatcgacga cgttctcgac gtgtcgcgca tcgtctccgg gaagctgcgt ctgaccgttg
    23521 aagatgtcga gatcgaccat gtgctcgccg ccgcagtcga gacggtgggg ccgagcgcag
    23581 atgcaaagat gatcacgatc gagaaggagc tcgcgccgca gctcgggttg atcgtcgccg
    23641 acgccacgcg gctgcaacag gtggtctgga atctgcttac gaacgccgtg aaattcacgc
    23701 cgcggagcgg ccggatcacc cttcgtgcac ggcgcacgtc gtctcatctt caaatcgccg
    23761 tcaccgacag tggcgccgga atcgatcccg gctttctgcc gtatgtcttc gagccgttcc
    23821 ggcaggccga gaacccgagg acgcgcgttc acggaggatt gggattggga ctgtcgatcg
    23881 tccgctacct ggtcgaggcg cacggcggga ccgtggccgc ggagagtgcg ggccgcgatc
    23941 agggctccac attcacggtc acgcttccgc tgggtgcgct ggcctcgcag cgtcccgagc
    24001 cgatcacctt ctccgcgctt ccctcagtta cggaccttcc gaaaaagctc ggcggcgtga
    24061 cgatccttct cgtcgatgat gacgtcgaaa gccgcaggct ggtacacacg gtgctgaccc
    24121 gcgcaggcgc gcaggtgacg gtgacggcat cgggaccgga agcgctcgag tcgatcaagc
    24181 agcgcacatt tgatctcgtc attacggata tcgccatgcc gcacatggac gggtacacgc
    24241 tggcgcgcaa gatccgggag caggtgccga accagaagat cgtcgccttg agcgcctttc
    24301 ccgccggccg cgccggcatg gccgacaccc cgttcgatgt ctacctgttc aagccgatcg
    24361 agccggtgga actggtggat gcgatcgcgg ccgcggtgta gcgacggatc aatcgctgga
    24421 ggctgttgtc tcgtcatcgg ctacgatgat cgccacgaat tccggaaggt gagatgagag
    24481 caaacgcggc ggttagcagg gcaagtactc ggattgtgtt cgcagttgtg gccgcggtgt
    24541 tcgcactgac ggcacacgcg cagacgctga cggtctatga cgatgcgctg cagaatggct
    24601 ttgcagacta ttcgtttggt ggcgggacga acctgaacga cacgccgttc gttcacaccg
    24661 gcacgaaatc agcgtcgatc ctcggccaca atttcaatgc gctctcgttt gcccatgcac
    24721 cgggaaacgt tctcacggca ctgcatacgt ccgacacgcc catccttcgg ttctgggtta
    24781 atggcggcac cgcttcgggg cagcagttcc acttcagcct gcagttgaac ggagcgttcg
    24841 tcggaagtgc tgcggccctc gacacgtaca tcagcggtgg gggagtagcg cagaacgtct
    24901 ggcgtcaggt gacgatcgac ctccgccagg caccgttcaa cgccgtcgac ttcgatcgca
    24961 tcaaccttca gagcgatgag gatgccgcgc acaccgatgc cagcgcgacc tatttcgacg
    25021 atttcgttct cggccagccc accgcctctg ccgtcaaccc gatgcagatc gagcatgacg
    25081 tagtggttca tgcgatcacc agcgaccgat ttacctggcg cgacagcagc ggtcagccgc
    25141 gtgtggcgtc actcgctcat aacgacaacg agacagaagg cggatcgcac ggtggtgcgc
    25201 ttcgcgaatt caagtatcag ctctccaacg gccagacccg caccgctacc gtgaccacat
    25261 acggcaatgc cgggtatggc ggtttcggct acgtgaccat gcactcagct cactatgccg
    25321 gctgcaacgg tgatgactca ccgctcggca tgggtttcgc gggtcattgg cagcgcatct
    25381 tcgaaggaaa gcatcacgcc atttttcgtt tcacccagct ctatccgcgc aactgtccga
    25441 cctccggctc gatcatcacg cgctatctcc cggtcacgat cgattgggtc ttctcaacgg
    25501 gtcacgacaa tccattgtgg accgtcacct acgacatcga ccagatcgcc gcaacgggag
    25561 gtcagccggt ggccgcgaat atgtattggg acgattcccg cgcgccatac ggtgagctca
    25621 acatcgacgg cgacggcggg acggacctga atggagttgc ctggggcgat ttctacaagt
    25681 tcaccacaac gaacgccacc ggaacggcca tcacatggaa cagccccttc gactggacgc
    25741 agcagaatac ggtgcccttc gtgaaggagt ggctcgacgc gccgctcacc gcggataatc
    25801 cggcgaagca cgacgcgacg atcggaatcg tgcagacaca aatgatcagg cagcaggacg
    25861 ctggcggtgc ccgcgaccag agtgtaggca gcgacatcac tccgtactgg cacaagacgc
    25921 aatcagacgg cgtccagagc gccggccccg gacacgcgct tcccaatggc gacaactggc
    25981 cgtatcaggc ctgcggagac aacctttacg ccgatggcat cacgacgaac aacgcgcgga
    26041 tgacctggaa gacgcaattc ggattcgccg gccagagcac ctacacgatc aacaatggcg
    26101 ccgtcgaatc gtcagccgat accgcggccg gttatccgaa gaagagttac tccacctaca
    26161 tcgttctcgg gcagcacagc acgctgccgg tggacgccca gatcgcgcag gtcgagacgg
    26221 ttcagtcgct cacacttacc gcggcgaccg gcactgtggt cacgtccggt cccgccggag
    26281 tgacgcgcgg cgacacggtc ccctatgcac ccgccggata caaccacgtc tatggcgcgc
    26341 ttgcgttcca agccagcgga aacgcgctcg atgcgaacat ccaggttacg ccgataacga
    26401 cattcgatgt ctcggctccc ggtttctatt acgccttcaa cggcatcgcc ggacagaacc
    26461 cgccgctgtc tctggtccag ggtgttacct atcacttcaa catcaatacc accgcgagtc
    26521 a
//
LOCUS       DATLVW010000032         8532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_126234, whole genome shotgun sequence.
ACCESSION   DATLVW010000032 DATLVW010000000
VERSION     DATLVW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126234"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VNN25_01175"
     CDS             complement(<1..474)
                     /locus_tag="VNN25_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90163.1"
                     /translation="MTAEGNLKSTKATPLTAPTEAPGQLKIAWLVPDGSLLKKDEVIV
                     RFDPTDFETQLVAGREDHDTAGNKMLKTNSESSTTNTNLRRDAHQASDELLAARQFKF
                     DDAEIFSRYQRVESQIDETLAGRKKEHAEGVLGVRAKLSAADRDLVSIEQRKADLK"
     gene            complement(681..1916)
                     /locus_tag="VNN25_01180"
     CDS             complement(681..1916)
                     /locus_tag="VNN25_01180"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH90164.1"
                     /translation="MRKSLIGAGIAIAAVLAGAGWTRNKLAADRQGDWVRVSRGDLVT
                     GIDVTGTLSAVDSGSFGPPPVNDVWEYKISMIAPEGSDVKVGAPVLGFDTTDLQKRLD
                     EKSAERDQAHKEIEKNTADLALRREDEKLKLADAEARLRKAQLKLEGPPDIVSSNDRR
                     QIELDFALAKQETESIRQRIASFERAAAAQIALLTSKQLTAASVVNETQNAIKAMSIA
                     APRNGTIVYVVNWRGDKKKVGDTCWRMERVIEIPDLARMMAKGEVDEADAGKIAVGQR
                     VVFNLDAHPDEEFAGTIKEAGRTVQQQQGTKDPIKVLKVNIVLDRTDPAKMRPGMRFH
                     GTVELARAKSALLIPRSAVFVTDSGPVAYRRGAFSVTAVPLKLGRENEKTVEVVSGLS
                     ASDRVLVPKAADGKEPQKS"
     gene            complement(1909..3090)
                     /locus_tag="VNN25_01185"
     CDS             complement(1909..3090)
                     /locus_tag="VNN25_01185"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH90165.1"
                     /translation="MKMFRTCASVALALAAASCFSGYRTEADAKALRVRRGTFARDVV
                     LTGELRAARGEELAVPRLPQWQTTIKWIAPEGAEMKQGDRVIELDNSAFSANLDAKRQ
                     AVEQAQQQLQQKDAEWAADTLEKALDVERKRSEYDKAKIDASMPKEIISARDYNDRQI
                     KFKRAEVERAKAIDVLKSQTMSVKSDRDNLLLSLQKAQRELTTAEKAISELILRAPKN
                     GIVVIRDHPWEGRKLQEGDGVFVGWTLAQFPEMQSLRVEADLADVDDGRIGAGMPATI
                     VIDAYPGMSFPGRVTSISAVAQEASRTSLKRAFRVLVALDKLDSSRMRPGLSSRVIVH
                     AMLQRDALLVPRAALDFAGAKPRVHLATGKSVDIILGACNAQECVVKSGLTEGETLGH
                     A"
     gene            3298..4287
                     /locus_tag="VNN25_01190"
     CDS             3298..4287
                     /locus_tag="VNN25_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXH90166.1"
                     /translation="MANPKHVAKLKEGADAWNEWRMMKDEAPDLSGVELDGDSLDGYD
                     LHFADFSNAELEGVSFQGALCNDSWFKKATINRAMFYQAELKRSNFEDSLIGFTVFHS
                     CSLVESNFTAARLIRNVFADVSLRDVRGLDATRAPRNNSLGIDTFFRSRGLPESFLRS
                     SNVPEAFIQYAASLVGKAIEYYSCFLSHSSKDGEFARRLYNDLQGKNVRTWFAPEDLK
                     IGDRFRSRIDESIRLHDKLVIILSANSVNSDWVESEVESALERERKEGKDVLFPIAID
                     DEGFTSQQSWAADIRRKRHIGDFRKWKSHDDYTNAFDRLVRDLKKSQPPIIMR"
     gene            complement(4863..5984)
                     /locus_tag="VNN25_01195"
     CDS             complement(4863..5984)
                     /locus_tag="VNN25_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXH90167.1"
                     /translation="MSELLVAPEPTKKQQEWRLAGVVFFAILALGILYATFVILEPFL
                     APIMLGAVLATVTFPVYRRLRDRMHGANRAASVMLILITFTVLLPAFLIIVLLVQEAN
                     VVVKHLQSGDMERTLQRLDISNRMPWLKRVAPNFDPAAINPAKVIIPVVQRAPAWIAA
                     HGASVIGGVAGLVIALFLVLLSAYFFYVEGETIVAEASALSPLPARYNAQFAENFKGV
                     VDATFRGQVITSLTQGIFIGIGLAVAGIPGAILWGAVATVLSLLPVVGSAAVWIPAAL
                     YLWVAASIGDRSYFGAIFLTVWGVVVVPIIEHVVRPWAMKGKMQLPAIPLLVSVLGGM
                     EAFGFVGLVVGPLVFSLLISVIDIYKRSFRIPPSEGIVA"
     gene            complement(6000..6617)
                     /locus_tag="VNN25_01200"
     CDS             complement(6000..6617)
                     /locus_tag="VNN25_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH90168.1"
                     /translation="MRAHPAFSSNALESHAPDVLRVVVVSTAPLVRASLRTILGEFDD
                     LDVTEAEPSRLRAVNAEVMLCDGNAPDVDVPALVLVRDAAGAAEALACGARGVLARTA
                     APRRMHAALHAVAEAEIVIDDAFADSLLRNPRAEVEMIEPLTAREREVLRLLASGQTN
                     KEIGQRLGVTDHTIKFHVNGILGKLGAQTRTEAVVEAARRGIIAV"
     gene            6879..7427
                     /gene="rfbC"
                     /locus_tag="VNN25_01205"
     CDS             6879..7427
                     /gene="rfbC"
                     /locus_tag="VNN25_01205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HXH90169.1"
                     /translation="MNIRETTLPGVVIIEPKVFGDDRGFFMETYRLDAFRDAGIDAVF
                     VQDNHSRSAKGVLRGLHYQEPNAQGKLVRCTRGALYDVAVDIRRGSPSFGRWFGTELT
                     ESNKRMLWVPPGFAHGFCSLEDGSDLVYKCTTLYDPPADRAILWNDPDIGIEWPIASP
                     QLSPKDAAAPRLRDATFLPTHP"
     gene            7512..7588
                     /locus_tag="VNN25_01210"
     tRNA            7512..7588
                     /locus_tag="VNN25_01210"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7546..7548,aa:Asp,seq:gtc)
     gene            7684..7815
                     /locus_tag="VNN25_01215"
     CDS             7684..7815
                     /locus_tag="VNN25_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90170.1"
                     /translation="MGSLRAEASFSTLDYYEVAYSQILSMQVRHGANVLITIMNDAC"
     gene            7917..>8532
                     /locus_tag="VNN25_01220"
     CDS             7917..>8532
                     /locus_tag="VNN25_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90171.1"
                     /translation="MAHVTFVHGIANKPAADRLLQIWLKALAFGDGIDLGANGVTSSM
                     VYWADVMYAAPLPPEEASEESVGGGAAPLRLESAEMAGIMPLTGDVDMSWLANASPEE
                     LQLVNRIAAEVAVKSGDPAVAVRAELSAASVAVGAQLERIPLPWFVKQPLMRILLRDV
                     HHYLFNAESTPRPKETFRVRDEIRRRMLAKLQEGAMKERPHVVVS"
BASE COUNT         1669 a         2816 c         2441 g         1606 t
ORIGIN      
        1 cttcagatcg gccttgcgct gctcgatcga aacgagatcg cggtcggccg cggaaagctt
       61 ggcgcgcacg ccgagtacac cttccgcgtg ctccttcttc cggcccgcca gagtctcgtc
      121 aatttgcgac tcgacgcgtt gatagcgcga gaaaatctca gcatcgtcaa acttgaattg
      181 ccgcgccgct agcagctcat cgctggcctg atgcgcatcg cggcgaaggt tcgtgttcgt
      241 cgtcgacgac tcgctgttcg tcttgagcat cttgttgccg gcggtgtcgt gatcctcgcg
      301 tcccgctacg agttgcgtct cgaagtccgt cggatcgaaa cgaacgatga cttcatcctt
      361 tttcaacaac gagccgtcgg gaacgagcca ggcgatcttg agttgtcccg gcgcctcggt
      421 aggcgcggtg agcggtgtcg ccttcgtcga cttcagattt ccttccgcgg tcacgcggcg
      481 cgcgaaacga acgggctcga cacggaaagt agggacctcg gatccctgac gacacgaccc
      541 ggccagcagc gccagagcca cgagcgcgcc tcgccgagga gtcaagcgat tcaccacaga
      601 gacacagagg acacagagga gggtctctgt gctctctgtg cctctgtggt gattcttctc
      661 ctcggcattt aggtgccggc tcacgatttc tgcggctcct tgccgtccgc ggccttcgga
      721 accagcacac ggtcgctcgc cgagagtccg ctgaccacct cgaccgtctt ctcgttctcg
      781 cggccgagct tcagcggtac cgctgtcacc gagaacgctc cgcggcgata cgcaaccggc
      841 ccgctgtccg tcacgaacac cgcgctgcgc ggaatcagca gcgcgctctt cgcacgcgcc
      901 aactcgaccg tgccgtgaaa acgcattccc ggccgcatct tggcggggtc ggtgcggtcg
      961 aggacgatgt tcaccttgag caccttgatc ggatccttcg ttccctgttg ctgctgcacc
     1021 gtgcgcccgg cttccttgat cgtgcccgcg aactcctcgt ccggatgcgc gtcgagattg
     1081 aacaccacgc gctgtcccac cgcgatcttg cccgcatcgg cttcgtcgac ctcgcccttg
     1141 gccatcatgc gggccaggtc agggatctcg atgacgcgct ccatccgcca gcaggtgtcg
     1201 cccaccttct tcttgtcgcc gcgccagttg accacgtaga cgatcgtccc gttgcgcggc
     1261 gcggcgatcg acatcgcctt gatcgcgttc tgcgtctcat tgacaaccga tgcggccgtc
     1321 agttgcttgc tcgtcagcaa cgcgatctgc gctgcggctg cacgctcgaa cgaagcgatc
     1381 cgttgccgga tcgattccgt ctcctgcttc gccaacgcga agtcgagctc gatctgcctg
     1441 cggtcattcg agctgacgat gtcgggcggt ccttccagct tcagttgcgc cttccgcaac
     1501 cgcgcctcgg cgtccgcaag tttgagcttc tcatcctcgc ggcgaagcgc cagatcggct
     1561 gtgttcttct cgatctcttt gtgcgcctga tcgcgttccg cgctcttttc gtcgagccgc
     1621 ttctggagat ccgtcgtatc gaagccgagg accggcgctc ctaccttcac gtcactcccc
     1681 tcgggggcaa tcatcgagat cttgtactcc cagacgtcgt tgactggcgg cggtccgaag
     1741 ctgcctgagt cgaccgcgga gagcgtgccg gtcacgtcga tgccggtgac cagatcgccg
     1801 cgcgacacgc gaacccaatc accctgccgg tccgcggcca gcttattgcg cgtccatccc
     1861 gctcccgcca gaacggcggc gatggcgatg ccggcaccga tcagcgactt acgcatgccc
     1921 cagcgtctcc ccttcggtca atccgctctt cacgacgcac tcctgcgcgt tgcaagcgcc
     1981 gaggatgatg tcgacgctct tgccggtggc aagatgaacg cgcggcttcg cgccggcgaa
     2041 gtcgagtgcc gcgcgcggaa cgagcaacgc atcgcgctgc aacatggcat gcacgatcac
     2101 acgcgacgac aggccggggc gcatgcgcga cgaatcgagc ttgtcgagtg cgacgagcac
     2161 gcggaacgcg cgcttcagcg aggtgcggct ggcctcctgc gccaccgcgg agatcgacgt
     2221 gacgcggccg ggaaacgaca tccccggata ggcgtcgatg acgatcgtcg ccggcatccc
     2281 tgcgccgatt cgtccgtcgt cgacatcggc gagatccgct tcgacgcgca acgactgcat
     2341 ctccggaaat tgcgccagcg tccatccgac gaagactcca tctccttcct ggagtttccg
     2401 cccttcccat ggatgatcgc ggatgacgac gattccgttt ttcggcgcgc gaagaatcag
     2461 ctcgctgatg gctttctcgg cggtcgtcag ctcgcgttgc gctttctgca gactgagcaa
     2521 cagattgtcg cggtccgatt tgaccgacat cgtctgcgac ttgagcacgt cgatcgcttt
     2581 ggcccgctcc acctcggcac gtttgaattt gatctgccgg tcgttgtagt cgcgggccga
     2641 aatgatctcc ttcggcatcg aggcatcgat cttggccttg tcatactcgg aacgtttgcg
     2701 ctcgacgtcg agcgccttct ccagcgtgtc cgctgcccac tccgcatcct tctgctgcaa
     2761 ctgctgctgc gcctgctcta ctgcttgacg ttttgcatcg aggttcgccg agaaggcaga
     2821 gttatcgagc tcgatgaccc ggtcgccctg cttcatttcc gccccctcgg gcgcaatcca
     2881 cttgatcgtc gtctgccact gcggcagccg cggaaccgcc agctcctcgc cgcgcgccgc
     2941 gcgcaactcg ccggtgagaa cgacatccct ggcaaacgtg ccacgccgca cgcgcaacgc
     3001 cttcgcatcc gcctcggtgc gataacccga gaagcaggat gctgcggcaa gcgcgagcgc
     3061 caccgaggcg caggttcgga acatcttcat catcgagatc ctcgattgta cgaccaactt
     3121 cgtacaacgc tggtaacgct acggttaaga cgcgtgcgac agaaaaaggt tagccgcccg
     3181 ctggtccatc tgcccatgta ctactggtta gacgtattgg acgcttactg gttacatcaa
     3241 gttagttcca gaacgcggaa gccgtcgccg aatttcgtgt ggaagtacac tctgcggatg
     3301 gcgaatccga agcatgtggc gaagttgaag gagggggcgg acgcgtggaa cgagtggcgg
     3361 atgatgaaag atgaggcacc cgatctttca ggagtggaac tggacggtga ttcgctcgat
     3421 ggctacgacc tacacttcgc ggatttctcg aatgccgaac tagagggtgt gagttttcag
     3481 ggcgccctct gtaacgactc ctggttcaag aaagccacaa tcaatagagc catgttctac
     3541 caagcagagt tgaagagatc gaacttcgaa gactcgctca tcggattcac ggttttccat
     3601 tcgtgctctc tcgttgaaag caacttcacc gcagcgaggc tcattcgcaa cgtattcgcg
     3661 gacgtctctc tgcgagacgt ccgcgggctg gacgctaccc gcgctccacg taacaactcc
     3721 ctcggcattg atacattctt tcgatcccgc ggcctcccag agtccttcct tcgcagctcc
     3781 aacgtccccg aagcgttcat ccagtacgcg gcatctctcg tcggcaaggc gatcgagtac
     3841 tactcctgct tcctcagcca ctcctccaaa gacggcgagt tcgcacgccg actctacaac
     3901 gacctgcagg gaaaaaacgt ccgcacctgg ttcgcgcccg aggatctgaa gatcggcgac
     3961 cgcttccgct cgcgtatcga cgaatccatt cgcctccacg acaaactcgt gatcatcctc
     4021 tctgccaact ccgtcaacag cgattgggta gaatcagaag tcgagtcggc tctcgagcgc
     4081 gagcggaagg aaggaaaaga cgtcctcttc cccatcgcca tcgacgacga gggcttcaca
     4141 tcgcagcagt cgtgggcagc cgacatccgc cgcaaacgcc acatcggcga cttccgcaaa
     4201 tggaaatcgc acgacgacta caccaacgcc ttcgaccgcc tggtccgcga cctcaaaaag
     4261 tcccagcccc cgatcatcat gcgctaagtg gtgggtcaat ttttaaaaga ccggtcacca
     4321 caaaggcaca aaggccacag agaacctccg tgtcctctgt gtgtctctgt ggttgccttt
     4381 ccttcggcga ccacgataag ccgtccaaat tgacccacga tcaatcgata atcaccgccg
     4441 ggcgagacgt cacccaaaag acaacgagtc tcttgcgaaa gaaccgtaag ttccatgatg
     4501 agacccgtga gtttcatgaa tgagacccgg agtctcatgc atgagaccag tgagcttcat
     4561 gaatgaggct cgtggacttc atgaatgagg ctcgggacct catgcgtgag actcggcgac
     4621 ctcatgcgtg agactcggcg acctcattca tgagactcgg gacttcatgc atgagactcg
     4681 ggacttcatg catgagactc ggggacctca tacatgagac tcggggactt catgcatgag
     4741 actcgggact tcatacatga gactcgggac ttcatacatg agactcggga cttcatgcat
     4801 gagactcgga gtctcatgaa gattgtgagg cagaagggtt aaaacacttc ccacgcagca
     4861 acttacgcca cgatcccctc actcggcggt atccggaacg atcgtttata gatgtcgatc
     4921 accgaaatca gcagcgagaa gaccagcggg ccgacgacca ggccgacgaa gccgaaggcc
     4981 tccatcccgc cgaggacgga gacaagtagt ggaatggcag ggagctgcat ctttcccttc
     5041 atcgcccacg gcctcactac atgctcgatg atcgggacga ccaccacgcc ccagaccgtg
     5101 aggaagatcg ccccgaagta gctgcggtcg ccgatcgatg ccgccaccca gaggtacagc
     5161 gccgccggga tccacaccgc cgctgagccg accaccggta gcagcgacaa caccgtcgcc
     5221 accgcgcccc acagaatcgc gccgggaatc ccggcgacgg ccagaccgat gccgatgaag
     5281 atgccctgcg tcaacgacgt gatcacctgc ccgcggaaag tggcgtcgac cacacctttg
     5341 aagttctccg cgaattgggc gttgtaccgc gccggcagcg gcgacaacgc cgacgcctcg
     5401 gccacgatcg tctccccttc cacgtagaaa aagtacgccg aaagaagaac gaggaacagc
     5461 gcgatcacca ggccggccac cccaccgatg accgatgccc cgtgcgcggc gatccacgca
     5521 ggcgccctct gcacgacggg aatgatcacc ttcgccggat tgatcgcggc aggatcgaag
     5581 ttcggcgcga cacgcttcaa ccacggcatc cggttgctga tgtcgagacg ttgaagcgtt
     5641 cgctccatgt cgcccgactg cagatgcttc acgacgacat tcgcctcctg caccagcagc
     5701 acgatgatca gaaacgccgg caggagaacg gtgaacgtga tcaggatcag catcaccgag
     5761 gccgcgcgat tcgctccgtg catgcggtcg cgaaggcgcc ggtagaccgg aaacgtgacc
     5821 gtcgccagca ccgcgccaag catgatcggc gcaagaaacg gctcgaggat gacgaacgtg
     5881 gcgtagagaa tcccgagcgc gagaatggcg aaaaagacga caccggccag ccgccactcc
     5941 tgctgtttct tcgtcggctc aggagccacc aggagctcgg acatgcgatc gataccgcac
     6001 taaaccgcga tgatgccgcg ccgcgccgct tcgacgaccg cctcggtacg cgtttgcgcg
     6061 ccgagcttgc cgagaatgcc gttcacgtgg aacttgatgg tgtgatcggt gacgccgagg
     6121 cgttgaccga tctccttgtt cgtctgaccg gaagcgagga gacgcaacac ttcgcgctcg
     6181 cgcgccgtca gcggctcgat catctcgacc tcggcccgcg gattccgcag cagcgaatcc
     6241 gcaaacgcat catcgatcac gatctccgcc tcggctaccg catgcaacgc cgcatgcatc
     6301 cgccgcggcg cagccgtccg cgcgagcacg ccccgggcgc cgcacgccag cgcttccgcg
     6361 gctccggcag catcgcgcac cagcacaagc gcgggaacat cgacatccgg cgcattgcca
     6421 tcgcaaagca tcacctcggc attcacagcg cgcaaccgcg aaggctccgc ctccgtgaca
     6481 tcgagatcat cgaactcgcc cagaatcgtg cgaagactgg cccgcaccaa cggcgcagtg
     6541 ctgacgacaa ccacgcgcag aacgtccggt gcatggcttt cgagagcgtt ggaactgaat
     6601 gccggatgcg cgcgcatgga gaaacaatac agcaaaacgg ggtgtggggg gtggggagtg
     6661 gggagtggtt ggagattcga gatcagagat caggggatca gagatcagga gatcaggaga
     6721 tcagagatca ggagatcagg agatctggcc ctggccgctg atccctgcta tctctgatct
     6781 ctgcacttct gatctctgca tctctgatct ctgcatctct gactcccaca ccccactccc
     6841 cacaccccac tccccacacc ttgctatcat ccgcgccctt gaacatacga gaaaccacac
     6901 ttcccggagt cgtgatcatc gagccgaaag tcttcggcga cgatcgcgga ttcttcatgg
     6961 agacgtatcg actcgacgcc ttccgcgacg ccggcatcga cgccgtcttc gtccaggaca
     7021 accactcccg ctcggccaaa ggcgtcctgc gcggcctgca ctaccaggag ccgaacgcgc
     7081 agggaaagct ggtgcgctgc acgcgcggcg ctctctacga tgtcgccgtc gacatccgcc
     7141 gcggctcccc atcattcggc cgctggttcg gaaccgagct gacggaatcg aacaagcgca
     7201 tgctctgggt cccccccggc ttcgcgcacg gcttctgttc cctcgaagac ggctcggacc
     7261 tcgtctacaa atgcaccacg ctctacgatc cccccgccga ccgcgccata ctctggaatg
     7321 accccgacat cggcatcgaa tggccgatcg cttcgccgca gctgtcgccg aaggatgccg
     7381 cggcaccgcg gctgcgcgac gcgacctttc ttcccaccca tccttgacac cccgctgccc
     7441 cacccgtagc atgcgcgcct ctcgggaatg ccgccagcag aggtcaggtt agcgaggttt
     7501 ccctacgacg cggggtcgta gttcagttgg ttagaacgcc ggcctgtcac gccggaggtc
     7561 gcgggttcga gtcccgtcgg ccccgccaaa ttaagcagtc atcagccgtc ggcctccgta
     7621 tttctagcat ccgctattca gcgaagagct gcctctccga taaacatctc attggctttc
     7681 tgcgtgggct cattacgggc ggaggcttct ttctccactc tcgactacta cgaagtcgcc
     7741 tactcgcaga ttctctcgat gcaggtacgt cacggcgcca acgtgctgat cacgatcatg
     7801 aatgacgcgt gctgagcgcc gcgtcagccg ggacatcgga caaaaaaagg caaaggcgtt
     7861 cccgaagccg gtagggaatc tcctctccca gtcactccct gacaggggag agttgcatgg
     7921 ctcacgttac gtttgtgcat ggcatcgcaa acaaaccagc ggcagaccgg ttgcttcaga
     7981 tctggctcaa ggcgcttgcg ttcggcgacg gcatcgattt gggagcgaac ggcgtcacgt
     8041 cgtcgatggt ctattgggcg gatgtcatgt acgccgctcc tctgccgccc gaggaagcga
     8101 gcgaggagag cgtcggcggc ggtgccgcac cgcttcggct ggagagtgcc gaaatggccg
     8161 gcatcatgcc gctcaccggc gacgtcgaca tgtcatggct ggcgaacgca tcgcccgagg
     8221 agctgcaact ggtgaacaga attgccgcgg aagtggctgt gaaaagcggc gatccggccg
     8281 ttgccgttcg cgcggagctc agcgcggcca gcgttgccgt cggcgcgcag ctcgagcgca
     8341 ttccccttcc gtggttcgtc aagcagccgc tgatgcgcat cctcttgcgc gacgttcatc
     8401 actatctctt caacgcagaa tcgacgcccc gcccgaagga gacatttcgg gtgcgtgacg
     8461 agatccgccg gcgcatgctc gccaagcttc aggagggcgc gatgaaggag cgcccgcacg
     8521 tcgtcgtgag cc
//
LOCUS       DATLVW010000033         3267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_497866, whole genome shotgun sequence.
ACCESSION   DATLVW010000033 DATLVW010000000
VERSION     DATLVW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497866"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..2755
                     /locus_tag="VNN25_01225"
     CDS             281..2755
                     /locus_tag="VNN25_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009049076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90172.1"
                     /translation="MWNRILESKPPAPGTVLDEAKAAGRDAKSMPGADEDYYADMDYG
                     VTKNPEAVRAALDPYVPGITADAAVKSAVRGRNNWIVWTAGNDRLWDELSRQSAGYLD
                     LLKTLSNHPSLQFSRANRWSVLGLVNEPCFKKSSGPRADRWGLWLDERVEGNGCGPDP
                     FENETKYPGVKSGARGTGKLALGSYYGYATGIVGLRLFPNPAFDEKAQAKWDANRYYT
                     DPTYYNDTKLVKPYRVAMSCGFCHVGPNPTNPPADPENPTWANLNSNPGAQYFWIDRI
                     FMWDHDASSFVFQLFHTSRPGALDTSLVSSDQINNPRTMNAIYDLGARLEIGRKWGAE
                     HLAGGSADNKQFNDYVPAGTPLTKFYDKGTQTAYTTHVLKDGSDSVGALGALNRVYIN
                     IGLFSEEWLLHFRPLLGGTALQPISPIKIADADKNSTYWQANIAQTPDLALFFLASAK
                     PDYLSNAPGGAAYLATDAALVPRGKEVFSERCARCHSSKLPEKAFANFFPPGSIGPDY
                     LKYWNEYWQWTKTQEFKDQMKAIVMSPDFLKDNYLSTELRVPSTLMETNICSPLATNA
                     IRDNIWDNFSSESYKNLPSVGKVTIHDPYTGEPSEYEMPAGGRGYTRPASLISVWATA
                     PYFQNNSLGSFDGSGSVAGREKTFEDGITQLLWPEKRQGSRSYVTASGKSHPGVIDVT
                     PGPTFLNVAPGFLPASLAPLSGTLDTFFPNVFNSGGVKIGPIPTGTPINLLSNIDLTK
                     GAEVLDLLLHIKHDLKSLPDNYTEEQARAQFAPLVPKLLGVSKCKDFIVNRGHYFGTD
                     FLPAKEGEPGLTDADKHALMAFLKTF"
     gene            2780..>3267
                     /locus_tag="VNN25_01230"
     CDS             2780..>3267
                     /locus_tag="VNN25_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXH90173.1"
                     /translation="MADVTAEYEYVVVGSGAGGGTVAARLAESGFKVLLLEAGGDPVT
                     LQGGDPNDPTGNRLPDDYNVPVFHPFASENDAMKWDFFVRHYKDNALQRRDEKAVDTP
                     DGPRILYPRAGTLGGCTAHNAMIMIYPHNDDWRFVEDLTGDRSWSPDNMRKYFERMEN
                     CH"
BASE COUNT          708 a         1105 c          904 g          550 t
ORIGIN      
        1 gaatcggcgc gatcgtcgta aagggtcacg tcctggagat tccaagcatg actcgccttc
       61 gcgaactggc gaagatgtag cgggccgctt cgtccgatca gaccgatcag accgatccga
      121 ccgattcgag caaaaacaga actttgggtg gggaaaccgt cggcccaatc actcctaaat
      181 atagaaggat gaggttgccc gcctcgtcag ggagggatcc gtgaaaaagc tcctgctcac
      241 gatcattatc atcgtcatcg tcgtccttgg cggtctctac atctggaaca ggattctgga
      301 gtccaagcca ccagcgccgg gaacggtcct ggacgaagcc aaggccgccg gacgcgacgc
      361 gaagagtatg ccgggtgccg acgaggatta ctacgccgac atggattacg gcgtgacgaa
      421 gaatccggaa gccgttcgcg cagcactcga tccgtacgtt cccggcatca ccgccgatgc
      481 agccgtgaag agcgccgtgc gcgggcggaa caactggatc gtctggaccg caggcaacga
      541 ccggctgtgg gacgagctct cgcgccagag cgccggatac ctcgacctgc tgaagaccct
      601 ctccaaccat cccagcctcc agttcagccg cgccaatcgc tggtcggtgc ttggactcgt
      661 caacgagccc tgcttcaaga aatcgagcgg accgcgcgcg gatcgctggg gcctctggct
      721 cgatgagcgc gtcgaaggaa atggttgcgg gcccgatcca ttcgagaacg agacgaagta
      781 tccgggcgtc aagagcggcg cccgcggcac aggcaaactg gcactcggct cctactacgg
      841 ttacgccacc ggcatcgtcg gactgcgcct cttccccaac cctgctttcg atgaaaaagc
      901 acaagcgaag tgggatgcaa accgttacta caccgatccg acctattaca acgataccaa
      961 actggtgaag ccgtatcgcg tggccatgtc gtgcggcttc tgtcacgtcg gtccgaatcc
     1021 caccaacccg ccggctgatc ccgaaaaccc gacctgggcg aatctgaaca gcaatcccgg
     1081 cgcacagtac ttctggatcg accgtatttt catgtgggac cacgacgcgt cgagcttcgt
     1141 attccagctc ttccacacct cgcgccccgg cgcgctcgac acgtcgctcg tctcgtccga
     1201 ccagatcaac aacccgcgca cgatgaacgc aatctacgac ctcggcgcgc gtctcgagat
     1261 cggccgcaag tggggcgcag aacatctcgc cggcggcagc gcggacaaca aacagttcaa
     1321 cgattacgtc ccggccggca cgccgctgac gaagttctac gacaagggca cgcagacggc
     1381 gtacaccacg cacgtgctca aggatggctc cgactccgtc ggcgcgctcg gcgcgctcaa
     1441 ccgcgtttac atcaacatcg gcctcttcag cgaggaatgg ctgctgcact tccggccgct
     1501 tctcggcggc accgcgctcc agccgatctc gccgatcaag atcgccgatg ccgacaagaa
     1561 ctcgacgtat tggcaggcga acatcgcaca gacgcctgac ctcgccctct tcttcctcgc
     1621 cagcgcgaag ccggattacc tgagcaacgc tccgggcggc gccgcctacc tcgctaccga
     1681 cgccgcgctc gtcccgcgcg gcaaggaagt cttctccgag cgttgcgccc gctgccactc
     1741 cagcaagctg cccgaaaaag cgttcgcgaa cttcttcccg cccggaagca tcggaccgga
     1801 ttacctcaag tactggaacg agtactggca gtggaccaag acgcaggaat tcaaggacca
     1861 gatgaaggcg atcgtcatga gtccagactt cctgaaagac aactacctct ccaccgaact
     1921 gcgcgttccg tcgacgctga tggaaacgaa catctgcagt ccgctggcaa cgaacgcgat
     1981 ccgggacaac atctgggaca acttctcctc cgagtcgtac aagaacctcc cctcggtcgg
     2041 taaggtcaca atccacgatc catacaccgg cgagccgtca gagtacgaga tgccggccgg
     2101 cggccgcggc tacacgcgtc ccgcctcgct gatcagcgtc tgggcgacgg caccctactt
     2161 ccagaacaac agcctcggca gcttcgacgg cagcggttcc gttgccggcc gcgaaaagac
     2221 ctttgaggac ggcatcacgc agttgctctg gcccgagaag cgtcaaggca gccgcagcta
     2281 cgttaccgcg tccggaaaat cgcatcccgg agtcatcgac gtcacgccgg gaccgacgtt
     2341 cctcaatgtc gcgccgggat tcctccctgc ctcactggct cccctttccg gaacgctcga
     2401 cacgttcttc ccgaacgtct tcaactccgg cggcgtcaag atcggaccga ttccgaccgg
     2461 cacgccgatc aaccttctct ccaacatcga cctgacgaag ggcgcggaag tgctcgacct
     2521 tctgcttcac atcaaacacg acctcaagtc tctgccggac aactacaccg aagaacaggc
     2581 cagagcgcag ttcgcaccgc tcgtaccgaa gctcctcggc gtcagcaagt gcaaagactt
     2641 catcgtcaac agaggacact acttcggcac cgatttcctg ccggcgaaag aaggcgagcc
     2701 cggtctcacc gacgcagaca aacacgcgct catggcattc ctgaagacgt tctgatcgcc
     2761 acctggacag aggagctcaa tggctgatgt gaccgcggaa tacgaatacg tcgtcgttgg
     2821 gtcaggcgcg ggaggcggta cagtcgccgc ccgcctcgcc gaatccggtt tcaaagtcct
     2881 gctcctcgaa gccggcggcg atccggtgac cctgcagggc ggcgatccga acgatccaac
     2941 tggcaaccga ctgccggatg actacaacgt ccccgtgttc catcccttcg cgtccgagaa
     3001 cgacgcgatg aagtgggact tcttcgttcg tcattacaaa gacaacgcgc tgcaacggcg
     3061 cgacgagaag gccgtcgata ctcccgacgg cccgcgcatc ctttatcccc gcgccggcac
     3121 gctcggcggc tgcaccgcgc acaacgcgat gatcatgatc taccctcaca acgacgactg
     3181 gcgcttcgtc gaagatctca ccggtgaccg ctcctggtcg ccggacaaca tgcgcaagta
     3241 cttcgagcgg atggagaatt gccacca
//
LOCUS       DATLVW010000034         3376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_415031, whole genome shotgun sequence.
ACCESSION   DATLVW010000034 DATLVW010000000
VERSION     DATLVW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415031"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VNN25_01235"
     CDS             complement(<1..566)
                     /locus_tag="VNN25_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90174.1"
                     /translation="MVQDPYSTVFARISALDEAARMAMAGLYLANYDGTSEALFLSDL
                     AKKDEALLVYSGPQLVGFTTLMLFEREWRSERIRVVYSGDTVVDRAHWGQQALAFDWI
                     GRMGGIKRERPDVPLYWFLLVKGHRTYRYLPLFGKSFHPHWSVDRSDLKPLADALATE
                     MFPDDYNPATGVVEFRRSRGHLKADIALP"
     gene            complement(1033..1437)
                     /locus_tag="VNN25_01240"
     CDS             complement(1033..1437)
                     /locus_tag="VNN25_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HXH90175.1"
                     /translation="MANTSKRGFASMDTSKQREIASKGGRAAHAKGTAHEFTSDEARL
                     AGRKGGEAVSRDRAHMSAIGREGGHSRGRTRQPMTGDDANASTGSGTDTERQNYIGHA
                     SDRESFGEPERRPMRQDNNEIRSDNNPGRGTL"
     gene            1737..1829
                     /locus_tag="VNN25_01245"
     tRNA            1737..1829
                     /locus_tag="VNN25_01245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1771..1773,aa:Ser,seq:gct)
     gene            complement(1856..2065)
                     /locus_tag="VNN25_01250"
     CDS             complement(1856..2065)
                     /locus_tag="VNN25_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90176.1"
                     /translation="MVSEKSGRWIVAAERLTANPKDRVPCPSCKAGYLETTTADFPDG
                     SHTDVYMKCPACGSANVLTYMKEER"
     gene            complement(2363..>3376)
                     /gene="thrC"
                     /locus_tag="VNN25_01255"
     CDS             complement(2363..>3376)
                     /gene="thrC"
                     /locus_tag="VNN25_01255"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXH90177.1"
                     /translation="QSDSLRAIVGLESPTYVSPPTPDTLYFKHEGENPTGSFKDRGMT
                     VAMTQAKRLGARAVACASTGNTSASLAAYAAQAGIRPVVFIPAGKVATGKLAQTLAYG
                     ATALHVRGDFDAAMTLVREACDKLGIYLVNSINPFRIEGQKTIVWELLQDLEWDAPDW
                     IVVPAGNLGNTSAFGKALREAFDAGWIRTMPRLASIQASGANPFFRSYRDSFATRHKI
                     TAETIASAIRIGDPVSHAKAVTAIQRTNGVVEEVTDDELMSAKRQIDEMGIGCEPASA
                     TTLAGVRKLRAAGVMRDGERIVCVLTGHLLKDADSILRNVPKERTIEIDPTIEAVEKA
                     LNT"
BASE COUNT          612 a         1014 c         1085 g          665 t
ORIGIN      
        1 ggaagggcga tatccgcctt gaggtgaccg cgcgagcggc gaaactccac gacaccggtc
       61 gcggggttgt agtcgtcagg gaacatttcc gtcgccagcg cgtccgccag cggcttgaga
      121 tcgctgcggt cgacgctcca atgggggtga aacgacttgc cgaagagcgg caggtagcgg
      181 tacgtgcgat gccctttgac gagcagaaac cagtacagcg gcacgtcggg acgctcgcgc
      241 ttgatgcctc ccatccgtcc gatccagtcg aacgcgagcg cctgctgccc ccagtgcgcg
      301 cgatcgacga cggtgtcgcc ggagtagacg acgcggatgc gctccgatct ccattcgcgc
      361 tcgaagagca tgagcgtcgt gaaaccgacg agctgcgggc cggagtagac gagaagcgct
      421 tcgtcctttt ttgccagatc gctcaggaag agcgcctcgg aggtcccatc gtagttggcg
      481 aggtacaagc cggccatcgc catcctggcc gcttcatcga gggctgagat gcgggcgaag
      541 acggtcgagt aggggtcctg aaccatgacc acacaccggc tgggatgcta gcacggcagg
      601 aaacggtgca tcgtgtccgc aaaaggtgac gatcgcacgg gtaaaaatgg aaactagaca
      661 atatcagaat ccgttaacgg taaaacggaa tccgttaacg gtaaatcaga atccatgaac
      721 ggtaaaccgg aatccgttca cggtaaatca gaatccatta gcggtaaatc gaaatccgtt
      781 aacggtaaat tggaatgagt gaacgggaaa tcggaatgag ttgacgggtt ctgcgaatgc
      841 attcacgtgc aaaaacctgt gtcacgcgga gccgcggaga ccgctgaggg gtttcgcgcc
      901 tcttcgtgca ctctcggcga gagcgtggcc gcttacccgc tccgaccccc gacccccgaa
      961 aggcgacccc cgcaaaaaga aaaaggaccg ccggtcctgc tggaacggcg gtccggaaga
     1021 gaggagaagg gcttagagcg ttccgcggcc ggggttgttg tcggagcgga tctcgttatt
     1081 gtcctgacgc atcggtctgc gctccggctc tccgaagctc tcgcggtcgc tggcgtggcc
     1141 gatgtagttc tggcgctcgg tgtcggtgcc gcttcccgtg ctggcgttgg cgtcatcgcc
     1201 ggtcatcggc tggcgtgtcc gtccgcggct atggccgccc tcgcggccga ttgctgacat
     1261 gtgggcgcga tcgcggctga ccgcttcccc acccttccgc ccggcgaggc gggcctcgtc
     1321 ggaagtaaat tcgtgagctg ttcctttcgc gtgagcggcg cgaccgccct tactggcgat
     1381 ctcgcgctgc ttcgatgtgt ccatcgacgc gaaaccgcgc ttgctggtgt tggccatgag
     1441 tgtctccttt gagggatgag tagtacagcg tgaactgcgc actacttgct tgcgtagcga
     1501 gctaatcagg cttcacgatc tgtgccagta agctccgcaa taactgggac gggggtgggg
     1561 tgtagggtgt ggggagtggg agagttgttt gagagttttc cagaaacgac tcgccacgtc
     1621 tcccactccc cgcaccctgc accccacccc ccatcccgta tctttgcgcg ccacgggaat
     1681 tgacccccct caatccgcat tctgctgcgt gcatttggca ccatcggttg agccacggag
     1741 agatgggtga gtggctgaaa ccaacggttt gctaaaccgt cgtagggcct aaagctctac
     1801 cgagggttcg aatccctctc tctccgccac cgcgcacggc gcgctgcact ctttttcagc
     1861 gctcttcctt catgtacgtc aggacgtttg ccgacccaca cgcgggacat ttcatgtaga
     1921 cgtccgtgtg cgagccgtcc ggaaagtcag cggtggtggt ctcgaggtag ccggccttgc
     1981 agctgggaca cggcacgcgg tccttcggat tcgcagtcaa ccgctctgcc gcaacaatcc
     2041 atcgccctga tttctcggaa accaaacgta acctcccgat caagctcctc aatcgtacgc
     2101 tccttcggca cgttgcgtag gatccccgcg gcgaggcgca caagtagcgc aggcattcct
     2161 gcctgcagcc gctgggcgtc cctgcccagc gggacttcga agcacgagtc tgcgtaccct
     2221 ccaccggcac gccaccatcc gagtgcggtt accgaaagac gaggccgcga ccggcgttcg
     2281 aaacgcgcca gtcgatagac tcaacgtttg cgcaataccg ctgggcgagg acgcccagcg
     2341 gctgcaggca agaatgcctg cactacgtgt tcagcgcctt ttccacggct tcgatcgttg
     2401 gatcgatttc gatcgtccgt tccttcggta cgttgcgcag gatcgagtcc gcatccttga
     2461 gaagatgtcc ggtgaggacg cagacgattc gttcgccgtc gcgcatgacg ccggcggcgc
     2521 ggagtttcct cacgccggca agcgtagttg ccgacgccgg ctcacagccg atgcccatct
     2581 cgtcgatctg gcgcttggcg gacatcagct cgtcgtcggt cacttcttcg acaacgccgt
     2641 tcgtgcgctg gatggcggtg accgctttgg cgtggctgac gggatcgcca atgcggatcg
     2701 cgctggcgat ggtctcggcg gtgatcttgt ggcgcgtagc gaacgaatcg cgatagctgc
     2761 ggaagaaggg gttggcaccg ctggcctgga tcgaggcgag gcgtggcatc gtcctgatcc
     2821 agccggcgtc gaaggcctcg cgcagcgctt tgccgaaggc gctggtgttg ccgaggttgc
     2881 cggcggggac gacgatccaa tcgggagcgt cccattccag atcctgcagc agttcccaga
     2941 cgatcgtctt ctgtccttcg atgcgaaacg ggttgatgga gttgacgagg tagatgccaa
     3001 gcttgtcgca ggcttcgcgg acgagcgtca tggcggcgtc gaaatcgccg cgcacgtgca
     3061 gcgccgttgc gccgtacgcg agtgtctgcg cgagcttgcc ggtggcgact ttccccgccg
     3121 gaatgaacac gaccgggcga atgccggctt gcgccgcata cgcggccagc gaggcgcttg
     3181 tgtttccggt cgacgcacac gccaccgcgc gcgcgccgag tcgtttggcc tgcgtcatgg
     3241 ccacggtcat gccgcggtct ttgaatgagc ccgttgggtt ttcgccttcg tgtttgaagt
     3301 agagggtgtc gggggtcggg ggggaaacgt aggtcggact ctctagtccg acgattgcgc
     3361 gcaaagagtc ggactg
//
LOCUS       DATLVW010000035         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_580540, whole genome shotgun sequence.
ACCESSION   DATLVW010000035 DATLVW010000000
VERSION     DATLVW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580540"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1075)
                     /locus_tag="VNN25_01260"
     CDS             complement(242..1075)
                     /locus_tag="VNN25_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH90178.1"
                     /translation="MSSYIKVGQENSTPIELYYEDHGSGSPVVLIHGWPLNGASWEKQ
                     TAALLAAGHRVITYDRRGFGSSSKPALGYNYDTFAADLDVVLSTLKLTDVSLVGFSMG
                     TGEITRYIGKYGAKRVRKAVLIGTLGPYLVKTADNPEGVDRSVFEGLKAAIKADRPAT
                     LMDFLRNFYNYDVTGGKLVSERVLEANWNVATGASAIGTLACIDSWIEDFRQDITRNT
                     VPTLILHGDADRVLPPDVSSRRQAKMIKNVRFVELKGGPHGVLWTHAEEVNSELVKFL
                     A"
     gene            complement(1273..1737)
                     /locus_tag="VNN25_01265"
     CDS             complement(1273..1737)
                     /locus_tag="VNN25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH90179.1"
                     /translation="MNQQKPDASAAKVAMNLEIEVIPVSDVDRSKQFYQLLGWRLDAD
                     DAPAKNVRIVQFTPPGSGCSVTFGKGITVAAPGSAVGALIVSDIKAAHDELIRRDIAT
                     SEMWHGASFPVEARLPGPDPKRTSYGSFFAFNDPDGNAWLVQEVTTRHPGRI"
     gene            complement(1799..2731)
                     /locus_tag="VNN25_01270"
     CDS             complement(1799..2731)
                     /locus_tag="VNN25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HXH90180.1"
                     /translation="MASAAAVADNPPLPTQIVDLANKVDGVHPGFRAFHAKGVVVEGT
                     FKASSEAARLSRATLFNGSPIPVTARFSDGSGMPTVPDGSPAMPRGIAIKYHLAGGGD
                     TDMVTNSFKFFPVGTGEDFRDLLQAITESPPDAPHPNKVEQFFGSHPNAPKAIGSLPI
                     PDSFADEEYHGIDAFVFVSKSGQRQAVRYVIAPEKLVHITPEEAAKQSPDYLFEDLAK
                     RIAQKPLVFHLKAQLAEPGDQTKDASLPWPDDRKVVDLGVLTLTKVVPNSAQAEKKLL
                     FLPTNLTPGIELSDDPLPRVRSAAYGVSYGRRSQ"
     gene            3184..>3417
                     /locus_tag="VNN25_01275"
     CDS             3184..>3417
                     /locus_tag="VNN25_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008038818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90181.1"
                     /translation="MTTKQKTIIVTKTTFSSCFRRVAAGLGASLALGALSAHAVPTPF
                     PADFRSSQVVNADATINVRVGGHGPAVVLIHGFG"
BASE COUNT          651 a         1018 c         1025 g          723 t
ORIGIN      
        1 agagttgacc gggcgcgaac gtcggattcg cgttcgcggc gactttcgat gcatccggga
       61 cgaagccaag cgactgcgcg acaggatcgg tcggatcgag cgccggcagg tcagtggccc
      121 ttgcatctgc cgcacctagc actggaatta ccagggcgga tgcaagcgca gactttagaa
      181 agtcacggcg gacaatggcg tgagacacgg gctgttcctc cgaaagtttg atgaactctt
      241 gctaagctaa aaacttcacc agttcggaat tgacctcttc tgcatgagtc cacagcacgc
      301 catggggacc gcccttgagc tccacgaatc tcacgttctt gatcatcttc gcctgtcttc
      361 tggatgaaac gtctggcggc agaacgcgat cggcgtcacc gtgcagaatg agggtcggta
      421 ccgtattgcg agtgatgtcc tgacggaagt cctcaatcca tgaatcgatg caggccagcg
      481 tgccgatcgc agacgcgccg gtggccacgt tccagttggc ctcaaggaca cgctcgctca
      541 cgagcttgcc tccggtgaca tcgtagttgt agaagttgcg cagaaagtcc atcagcgtgg
      601 ccggtcgatc agctttgatg gctgccttaa gaccctcgaa gacactcctg tccacgcctt
      661 ccgggttgtc ggccgtcttc acgagatacg gtcccagcgt gccgatgagc acggccttgc
      721 gcacccgctt cgcgccatac ttgccgatgt accgcgtgat ttcgcccgtg cccattgaga
      781 agccgacgag agaaacatct gtgagcttca gcgtgctaag cacaacatcg agatcggcag
      841 caaaggtgtc gtagttgtag ccgagagcgg gcttgcttga actgccgaaa cctctgcggt
      901 cgtaggtgat gacgcgatgg cccgcagcga gtagtgccgc cgtctgcttt tcccacgacg
      961 cgccgttcaa cggccatccg tggatgagga ccaccggcga tccggagcca tggtcctcgt
     1021 agtagagttc aatgggcgtt gagttttcct ggccaacttt gatgtaactg ctcatgggct
     1081 ctctctttct ctccgtttgt gtggactgta acgatctgcc aagtgcctcg gttcgagacg
     1141 tgcgcggcag cagcgccgcc gccatccccg ccgcgacgag cttgagccca tcgcggcggc
     1201 tgatgtggcc ttcggaactg ctcgctgcag ccgctgaaac atctttctcg ttcatgcaag
     1261 tttccctacg tatcagatac ggccggggtg ccgagttgtg acctcttgca ccagccaggc
     1321 gttgccatcg ggatcattga aggcgaaaaa cgacccgtag ctcgtacgct tggggtcggg
     1381 accgggaagg cgtgcctcca ccgggaacga tgcgccgtgc cacatctcac tcgtggcgat
     1441 gtcacggcgg atgagttcgt catgagccgc tttgatgtcg gatacaatga gcgccccgac
     1501 ggccgagccg ggcgctgcca cagttatccc cttgccgaac gtgaccgagc accctgagcc
     1561 gggaggcgtg aactggacga tgcgaacgtt cttagccggg gcgtcgtccg cgtcgagcct
     1621 ccaacccaag agttggtaga actgcttgga tcggtcgacg tcggacacgg ggatgacctc
     1681 gatctcgagg ttcatggcga ctttcgctgc gctggcgtca ggcttctgtt gattcatgtg
     1741 attttccgtt tgctaatctg tggtggtgat ttgccggccc cgtcgagcgt gcaggcatct
     1801 actggctgcg cctcccgtac gagacgccat aagcggcgga gcgtacgcgc ggcagcgggt
     1861 cgtcggagag ctcaataccg ggtgtgagat tcgtcggcag gaacagcagc ttcttctccg
     1921 cctgcgcgct gttgggcacc actttagtca gcgtcagcac gccgagatcg acgaccttgc
     1981 ggtcatcagg ccaaggcagg ctcgcatcct tggtctgatc gccgggctcg gccaattgcg
     2041 ccttcaagtg aaaaaccagc ggcttttgcg cgatccgctt ggccaggtcc tcgaacaggt
     2101 aatcaggtga ctgcttggct gcctcttccg gcgtaatgtg caccaatttt tccggcgcta
     2161 tcacgtagcg cactgcctgc ctttgaccgc tcttgctgac gaagacgaaa gcgtcaatgc
     2221 cgtggtactc ctcgtcggca aagctgtccg gaattggcag tgaaccgatg gccttcggcg
     2281 cattcggatg gctgccgaag aactgttcca ccttattcgg gtgtggggcg tcgggtgggc
     2341 tctcagtgat cgcctgcagc agatcacgaa agtcctctcc ggtccctacc ggaaagaact
     2401 tgaacgagtt ggtcaccatg tcggtgtcac ctccgccggc caggtggtac ttgatggcga
     2461 tgccacgggg catcgcaggc gagccgtcgg gcacggtcgg catcccactg ccgtcagaaa
     2521 agcgcgcagt caccggaatc ggactgccgt tgaatagcgt tgcgcggctg agccgcgcag
     2581 cttcggacga ggctttgaag gtgccttcca cgacaacgcc tttcgcatga aaagcgcgaa
     2641 accccggatg cacgccgtcg accttgttcg ccaaatcgac gatttgtgtc ggcaaaggtg
     2701 ggttgtctgc gacggcggct gcggaagcca acagcagcga tgcatacgtt aaggacctca
     2761 cgaggcgcct tgcgccggag cgtagggaac cttcaaagaa aatcccagtt gccccgctcg
     2821 aaactagtaa gcgcttcatt gattctcctg tcgtcgcgtg attacacggt tgcgattagg
     2881 acagaagcgc cgcgagtcca ccattgtgcg atcgtatagt tcgtgcgtac agatacgatg
     2941 cgatcccgtt tccaccccat gcattcatac catcgtgcaa tggcgcactt accgcgcgta
     3001 tgcctacatg tgatctgtcc ttcgagagta gagcaatcat gaaaagcggg ttcttcattt
     3061 cttcgctgga ctgttttgct tctccatgtg gacgcgcgat ctgttcgccg cggaatcctc
     3121 tgagggacac ttcatgattg cgattttcag tccaacagcc acaggcgaag gcggaaaact
     3181 cagatgacta cgaaacagaa gaccatcatt gtgacgaaaa cgacgttctc gagttgcttc
     3241 cgccgagtcg cggccggcct cggcgccagc ctggcgctgg gcgccctgtc ggcgcacgcc
     3301 gtgcccacgc ccttcccggc cgacttccgc agctcgcagg tggtgaacgc cgacgcgacg
     3361 atcaacgtcc gcgtgggcgg ccacgggccc gcagtcgtgc tgatccacgg cttcggc
//
LOCUS       DATLVW010000036        22107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_126413, whole genome shotgun sequence.
ACCESSION   DATLVW010000036 DATLVW010000000
VERSION     DATLVW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22107
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126413"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2341
                     /locus_tag="VNN25_01280"
     CDS             <1..2341
                     /locus_tag="VNN25_01280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH90182.1"
                     /translation="FHRGEETLAVHEPLAVTRGGFLIFRDSIARFADEGTHAWASLLR
                     RDRIEIPAKEIDEFIKETAVSKSPVRIDFPAAVAWKESLGVPHAVIEVRVPSWHDYVN
                     AIPNFEYGGFSVKGLVRTRAIYDEKTKSIIVRDLAEESRKLDVLWVQTFKRTGDYVSI
                     SPERFDECIPRLIAAGWEVRGAEGTYAGFGELDLSLRSSIDWFDLDANVTFGEQRVRT
                     PRLLAALRKGEAFVKLDDGRLGMIRPEWREQLEPFLATASEEHAEGLRFRRNQVLLLD
                     AMLATRKNVAFDDVFEQARRKILDVVVQPEQPPDSFTGELRPYQSEGLGWFTFLRSLS
                     FGGCLADDMGLGKTIQVLALLDQRRNLPKKEHRPSLVVVPRSVVFNWKKEAARFTPKL
                     RVLDHATPDRIRATDHFLEYDVVITTYALLRNEVELFADFEFDYAILDEAQAIKNASS
                     QAAKAATLLRARHRLALSGTPVENHLGELASLFEFLNPGMLRAKTTRVTKMLRDAGGK
                     TAQAAEARVMLARTVRPFILRRTKAQVATELPDRVEQTVYCDLEPKQRKLYDELRDFY
                     RASLLGRVAEKGIAKSKIHILEALLRMRQAACHPGLVDAKRTGETSAKLDKLFEELPI
                     LIDERHKVLVFSQFTSLLAIVQREMRQRGINYAYIDGKTRNREAEVSRFQEDSEIPVF
                     LISLKAGGLGLNLTAADYVFLLDPWWNPAVEAQAIDRAHRIGQSRNVMAYRLVARDTI
                     EEKILLLQQSKRALAEAIIGEENSILRQLTAEDLDLLLS"
     gene            2459..3646
                     /locus_tag="VNN25_01285"
     CDS             2459..3646
                     /locus_tag="VNN25_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90183.1"
                     /translation="MRTIILALIVLSLTSALSAQNVVVGTQSLGGTIAQSAAPPFTMI
                     DLSHPATADGSVASASVRWAAGSCTAAFKLKFLRPASPSSLTTFTLVAERGPFNAVAG
                     RNQLVLTPPVDVHAGDLIAVTSLGTFAACGSPATLIDSGGALMRISGDVSTGSFNGFY
                     TRDRSLGARATDTTEVLEGVVTAAGSLAGNFGSFFRTSIQIASPGGGTSSGTLVFHPA
                     GVAASPIDQAIPYTATSGNAISFTDIVQQMGKTGLGTIDVISNNGFPPIVTARIYNDT
                     PNGTSGFTEEMITPRDALHFGDTGVLLTPPDLTKFRVNIGVRTFSSPATINVQYGFRS
                     QSNMDFPANTFRQYSLAEFGDASPLANEQIILSMISGDAIVYLSTTDNQTNDSSVRFA
                     RRN"
     gene            complement(3643..5505)
                     /locus_tag="VNN25_01290"
     CDS             complement(3643..5505)
                     /locus_tag="VNN25_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXH90184.1"
                     /translation="MSELRYLRFGPFRLDRRERLLMRDGQPAALPPKAVDLLIALTAE
                     PGALRTKEELLAQVWPDVVVDESNLSQTIFVLRKALGDDDGQWIATVPRRGYRFSGKV
                     SADDSAAMPAPAEAQGSEPNRRWNRSRLVIAAAIAVILIAAATIAIIKLRKRHQGDAI
                     HSIAVLPFRPLDEQRGDRPLELGIADTLITKLSVLPDLIIAPTRAVAAFVDHPVDSLA
                     AGRTLDVDGVVEGSIQRVGQRIRCNVRLLRVSDGRAILADEYDEDASDLFVLEDRIAQ
                     RVADALDLRLSGRNRRDLARHSTKNAEAYQLYTQGRFQWNMFDQAGFLSSIRYYEAAL
                     RLDPRYADAWAGLANSYSVIGIYGPLPREESFMKAREAALRALALDPDLANAHAALGG
                     SGIFYTHNWREADEELHRALASDPNDVDAHSLRAYLLQAFARPEEAIAEVRRAHEIDP
                     TWIVPRNDLLKSLFFARRFDESIAEGRRAISLEPRNRFALNNTAFALISRGQRGQAAQ
                     LASAALAIAPNDARAVSNLGVIAARAGDRATAQKQIETLRQLRTHGDWRTDYEIATVY
                     AALGDNAAAFVALDDSLASHYPFIFEVHIDPRLDPLHNDPRFAPFLAKLGLDQR"
     gene            5730..6299
                     /locus_tag="VNN25_01295"
     CDS             5730..6299
                     /locus_tag="VNN25_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90185.1"
                     /translation="MRQAFSIDQRRGNARAIVTTRRAAVLVALVALGACNRVAPSAAS
                     ASRVASTSVVENNDSSAPAFEDKVPDIEACALLDRVDVIRIFGPLRDEAKLDRGLRGE
                     RDCRWHNEEGQWLKASLYGASRWELEKAIVSEMHPRAIANAGDEAFSVKQGTDSVVHV
                     RKGGAVIEVSCSCGIEKSESLARIAAARL"
     gene            complement(6321..7157)
                     /locus_tag="VNN25_01300"
     CDS             complement(6321..7157)
                     /locus_tag="VNN25_01300"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HXH90186.1"
                     /translation="MRAVTDSALLTHTPALVKLDADHPGFHDPSYRRRRDEIAHAALT
                     YRDGDPAPDVVYTDAEHEVWREVWRHLDSLHARYACREYLDAATRIALSRQQIPQLRA
                     VNTTIAPHFGFRMLPVAGLVTAEAFLKELGRGVFLSTQYMRHHSVPLYTPEPDVVHEL
                     IGHAATFVHADFAQLNRAFGRAALTASASDIERIGRVYWYTLEFGVVREEKALKVYGA
                     GLLSSFGELGRFETEEWLTDFDVEVVADTPYDPTHYQDRLFVAQSFDGMTRDVLAWLD
                     RF"
     gene            complement(7298..10447)
                     /locus_tag="VNN25_01305"
     CDS             complement(7298..10447)
                     /locus_tag="VNN25_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXH90187.1"
                     /translation="MSDSDDIDDIRPPVAPNERTATSIINRVVAGSLRQRYLLLFVAA
                     LLLAAGIYSFKRLPVDAYPDLSPPQVEITAQWQGRAPEEVERLITVPLEVEMNGIPNV
                     EVVRSISLYGLSDVRITFQPGTDNYFARQQVFERLAGVSLPDGVAPDIAPLFSPSGLI
                     YRYVLDSPDRSPMELKTLEDWVIERQYKSVPGVADDSGLGGPTMQYQVLLDPAKVAAV
                     GLSVPLIVESLSANNSNAGGGFYSQGGQFFYVRGLGRLTTPEDIGNVVVAVHNGTPVL
                     VKDVAQVAIGEAPRLGQFGFNNRDEAVEGVILMRTGEQAQVVLDRVEKKTKELNDSIL
                     PKDVKIRPFYDRRDLIALTTRTVEDNLLRGMLLVLVILIFFLYDFRSGLIVAATIPLS
                     LLFAFICLDLKHVPANLLSIGAIDFGILVDAAVVMVENIFRRLAINHEDGDPLTTSEV
                     IVQAAAEVDRPIFYAVAVIIAAFLPIYALTGPSGALFRPMADTTIFALIGALLLSLTL
                     VPALCFWALRKVRERRNVVFEAIRSVYVRGLDACLAHPWLTTTVSVILFALSLLLIPG
                     IGAEFMPHLDEGSLWVRANMPSTISFEEAAKISPQVRTILHSFPEVTVVSSELGRPDD
                     GTNPTGFFNDEFFVGLKPYSQWNGRYHSKAQLIEAIDEKLKAFPGIVFNFTQPAEDAV
                     DEAETGLKSALAVKIFGPDLAVLEDRGNAIKAVLQKVRGIGEISVIRQLGQPSLTVTV
                     DRAKIARYGINVADINGLIEAAVGGTAATQVVQGERSFDLVVRLKPEFRQTPEAIGNI
                     LVATPGGQQVPLKELADVREVSGASFIYREDNSRYIGVQYSIKDRDLAGAVEDAQRQV
                     AKSVQVPTGYRVVWGGEYENYTASKKQLQIIVPVTLLVIFMLLFMLYSNFKFPMITVA
                     AVLISGPIGGLLALYLTHTPFSVSSGIGFIALFGVSVQTAVIFISYANELRQRGLGIA
                     EATREAAILRLRPIMMTALVAALGLLPAALSTGIGSDTQKPFALVIVGGLFSRLLISV
                     FLMPALYALVARPHDRLEV"
     gene            complement(10444..11592)
                     /locus_tag="VNN25_01310"
     CDS             complement(10444..11592)
                     /locus_tag="VNN25_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH90188.1"
                     /translation="MSKQQSNRSLQSIALIGASVVLVLTVASCSQETNSKTETEMKTA
                     STAVRNVRTEVVAVSRFHRSIEATGTVAFDQNRSTQVLSPISGPVARILVGFGSHVGR
                     GEAMAIVSSPDFAAAMSALRRAEATARNAKRVAELDRQLFKNDAIARRDMEQAETDAV
                     GAEADRDSALQQLRSLGVTQQTIDDIQQNRPVTNPGGTIRSPLAGTVVEKLISPGQLL
                     QAGTTPCFTVADLSNVWVLANVFESDLPFVKIGDTADVVTGAGVAPMKGRVSYIAALV
                     DPNTRAVSVRIDVPNPGEVLKRDLYVRAVIQSQQESQGLLVPVSAVLRDEENLPFVFV
                     AKPDGSFGRQSVQIGSRIGDQQEITGGLTAGQTIVVEGGLFLSTVEHS"
     gene            complement(11585..12883)
                     /locus_tag="VNN25_01315"
     CDS             complement(11585..12883)
                     /locus_tag="VNN25_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH90189.1"
                     /translation="MLKSAGFCILFVVGGISPFAYAQDVQPAGELLRLSRQQAIDQAL
                     ARNYGLIAAREQVEQARAQVVVAAAFPDPSFAADVSGLPHPLSPRSSTGSDQGVAVTI
                     PFPGKTSLRRAVATADLRVAEFNLAQLRQQVASQAVQAYDAILVAKRHADDLITSKEF
                     AQDFLQKTQARFMAGTVAKVDVVKAQVDVSQAENDLIANERTAATARATLNRLLGRSG
                     GAPLELTDDLTFPGSLAAVETLEKLAESSRPELQSLQKQLEGAKAATRLAGKFWAPDV
                     SVSFTRNAAEGTPTSFTSGVSFGIPILFWQHQRGEIAAAQHREAELTANISDIRVQIS
                     LDVQTAFANASIALRQAIFIRDQLLPEAKEVYRVASASYGLGGSSALDLLDAKRTLLD
                     AEGQYADALGAANDAEAALELAVGTSLPKMSTVNTGETHE"
     gene            complement(12930..13274)
                     /locus_tag="VNN25_01320"
     CDS             complement(12930..13274)
                     /locus_tag="VNN25_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90190.1"
                     /translation="MWRARRWLAGLMVVVFAAVEMYAAAPQLHVHLQAGALGTVQQHR
                     GLSLTTRSSGRNAAPNDCLACRTFSLATALISWTGVTPPSVNQTLALPTLTVFAASGF
                     HDDARGRAPPAC"
     gene            13450..13953
                     /locus_tag="VNN25_01325"
     CDS             13450..13953
                     /locus_tag="VNN25_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXH90191.1"
                     /translation="MPEKQTLRRARKDKAEGKAPSTQAGEFVREEIDHIREGKHGARS
                     AKQAIAIGLSKARRAGVNLPPPAKGTTSEETRQSAARALKNGRAGGNHKTSAKRSKAT
                     LKALKREPRAAGSHLALERQALSSSRKRGPAARQRAAEKAVETKGPAGLHRAAVKAAA
                     TRSRAHH"
     gene            13983..14183
                     /locus_tag="VNN25_01330"
     CDS             13983..14183
                     /locus_tag="VNN25_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90192.1"
                     /translation="MKKVRLSFPQMMFVIATRAALAGGVALLNADRVKRETRRRIGWI
                     LAGVGVATTVPAAMMARRNLVA"
     gene            complement(14192..14854)
                     /locus_tag="VNN25_01335"
     CDS             complement(14192..14854)
                     /locus_tag="VNN25_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXH90193.1"
                     /translation="MSTMTAHDEGLVHLSIGPVSLDGNLLIPHAASGLVLFAHGSGSS
                     RFSTRNRHVAAFLREGGLGTLLIDLLTAEEERVDERTGQLRFDINMLAGRLAGAVEWL
                     SQNPETKSLPVGLFGASTGGGAALVAAARRPDRVAAVVSRGGRPDLAGDSLPLVKAPT
                     LLIVGGNDEPVISMNQRAFEQMSAVKRIEIIPGATHLFEERGTLERVAELARDWFQTY
                     LH"
     gene            complement(14865..15533)
                     /locus_tag="VNN25_01340"
     CDS             complement(14865..15533)
                     /locus_tag="VNN25_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HXH90194.1"
                     /translation="MFDQPFRDRVEAGLLLGAELNRRFGHRADALVLALPRGGVPVGF
                     EVAEALNAPLDVFIVRKLGVPGQEELAMGAIASGGVRVLNREVIRYASISDRVIDTVA
                     KAEQQELERREKSYRGVRPPIDVAGKTVFVVDDGLATGSTMRAAVMALRNMNAHEVIV
                     AVPVAAKETCLEFRAIADDIVCLRTPSPFQAVGLWYDDFGQTTDEEVHALLDRAWKTL
                     GAAS"
     gene            complement(15686..18556)
                     /locus_tag="VNN25_01345"
     CDS             complement(15686..18556)
                     /locus_tag="VNN25_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH90195.1"
                     /translation="MQSVDAGILVGVAIPQESPRLPLPDGTNVGPYRILSLLASGGMG
                     DVYRARDERLGRSVALKVLPTGLTDDRDRLERFAQEAKSASALNHPHIVTIYEIGQAR
                     PSCIVQPIPGTDQRQRPEEIHYIAMELIEGETLREFIAGGRSLSRRLEILAQTAEGLG
                     KAHAAGIVHRDLKPDNIMVSTEGYAKVVDFGLAKLVEPTAGWNPIGADSPTMRALTQQ
                     GELIGTAGYMSPEQILGKVIDQRSDIFSFGCIVYETIAGAKPFNGDSFVDTLHEVLHA
                     TPPAIEHYDPLMQQELRRIVGKCIEKDREYRYQSIRDVALDLRAAAANAGNPTGPITL
                     PQLEQPKAAHGLRWLAAFAAAVVIGVIGWQIHLRMSASATTAAPVAHTALQRITTNGH
                     VTHLAISPDGRFAAYTLSDGTAGPTVWLQQIATGSRVVVLPPSPKMDYTGLAFSADGN
                     HLLASRYEGTIFGAVVKVPLLGGTPARIIGDADTAVTPSPDGKLLAVVRNVLEKGESR
                     LLLTAPDGSHERVLATLPLTEGMMMPSPAWSPDGTRIAISHGHSLFTIDVSTGAKTSI
                     PMATSEGAIRDVAWTSAGDTLLVTALDDRWAGHCQLLRVNLGTGAVTNVTDDSDDYAE
                     PHAIGKSVAAIQAKYQSAIWSVVPGTAPVQLTRGLGSFDGLSGLTTAPDRSIIYASAA
                     GGTADLWIANADGSDARQLTNDERLESRPVVTANGTTIVYATHSQGRSSLWRMNIDGG
                     QQREIVSAPVIHDFILTPDRKQIVYASGNDATSFSSLMSIPVDGGKPSTIATAGTILK
                     PMQITPDGRTVIYALLDGQAFRLFKVAMTGGPVTRLTSEASNYGSISPDGKLIASAWK
                     SPFNPKLAIISINGGAPTRIFDLSGHLYRWTPDGKGIVYIKHDGKQENLFMQPLAGGP
                     PTALTNFTEGSIENYEWSADGSRIVLTHYIKTSDIVLLGASS"
     gene            18853..19848
                     /locus_tag="VNN25_01350"
     CDS             18853..19848
                     /locus_tag="VNN25_01350"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXH90196.1"
                     /translation="MSSCSFKPAAELPQARNSRLTDAPIVSSLGPEPWKSPWWKTAKG
                     DGATFYYIVAIHILAAIGLVFFPTPGWKVIVASVALGWLGGLGTTVCYHRALAHTALR
                     LHPVVKHVLIFFAMFNGSGSPDSWTANHRQHHSKVETVDDISSPAIGGFWWAHLRWLW
                     QAGNVPLNRWCPDLNKPEYRFWNRIQVPMLAVSLFGGLVFGLPAFFWLGSMRLVASLH
                     AQCFVNSIAHMRENREHGEDSSQNMGWLAPVQFFQGENWHQNHHASPSSARFGWKFWQ
                     VDVGWYSIFMLELVGLATSVKRPSHREKAVSSVVAPQVVAPQLVPPQVVAFDTPA"
     gene            19924..20346
                     /locus_tag="VNN25_01355"
     CDS             19924..20346
                     /locus_tag="VNN25_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HXH90197.1"
                     /translation="MNCPRDAAELEVKSFGTTPVRTCPVCSGMFLAHGQLNQVAGPIR
                     GDLEFCTVDLDSFQHEDAYGPITCPNDGAPMGKVDFNIDTTIILDYCKKCLGFWMDGK
                     ELARINEEVKRLNEADAEVPDPLLVRISQFFWNLPLPH"
     gene            20467..22038
                     /locus_tag="VNN25_01360"
     CDS             20467..22038
                     /locus_tag="VNN25_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90198.1"
                     /translation="MRTTRFLPKIAGYAFASLLCALLAFMAGCKAETATNTDTSATTT
                     AAASTTETASTAATSSAAAAASGMSTFKPVPLPDPHIAGYAFPEPEPTIIDWTKTNNQ
                     KAINTHAWGIWTALTQETTETFANQKLRVFETWPDVDDLMAASAAPAGGIAAMATTPR
                     LAHRLKKPRQFEHGGGAAALGLAANPGESTVLAFVKYDPIGGDHIVKNKLFSASNLSA
                     MLAAGKQAIPDFPNPSLALKPVFQTLSGSTVGGGRYYRLSTWPGSPALTYNSKDKMYH
                     SIPFPQNLWKQCVWIDIQATGKGPGTGGVDTTCAADGSSRTPASTYSVDEFIHFQLTA
                     AEAAAENAEGQVPPAKAGDFSVLVAMHVTSREITRWTWETFWWVPNPDKPTAPSSPAI
                     AADRPPQLQGAPRNYAHCTAYDMENPATPLVGGNNNGDSIYCFNPYLEAPFTPSVLPK
                     SIPGNTMFQGKTVQTANNVGAQTNCMSCHGQANWNPPTQPNIYTGDQYIAMNAPQFKG
                     VLKADFLWSIPDNAK"
BASE COUNT         4177 a         6773 c         7133 g         4024 t
ORIGIN      
        1 gtttcatcgc ggcgaggaaa cgcttgccgt tcatgagccg ctcgcagtca cgcgcggcgg
       61 gttcctgatc tttcgcgact cgatcgcccg gtttgcggac gaaggaacgc acgcttgggc
      121 gtcgctgctg cggcgcgacc ggatcgagat tccggcgaaa gagatcgacg agttcatcaa
      181 agaaacggcc gtatccaagt caccggtgcg gatcgatttt ccggccgccg tggcgtggaa
      241 ggagagcctt ggtgttccgc atgccgtgat cgaagtgcgc gttccgtcgt ggcacgacta
      301 tgtcaatgcg attccgaact tcgaatatgg cggcttttcg gtcaaggggc tcgtcagaac
      361 gagggcgatt tacgatgaga agacgaagag catcatcgtg cgcgacctgg ccgaggaatc
      421 gcgcaagctc gacgtgctat gggtacagac attcaaacgc acgggcgact acgtatcgat
      481 ttcgcccgag cgtttcgacg agtgcatccc gcgattgatc gcagccggct gggaagtccg
      541 cggcgccgag gggacgtacg ccggtttcgg cgagctcgat ctctcgctcc gcagcagcat
      601 cgattggttc gatctcgacg ccaacgtcac gttcggtgag cagcgcgtcc gcacgccgcg
      661 ccttctcgct gcgcttcgca aaggcgaggc cttcgtcaag ctcgacgacg gacgcctcgg
      721 catgatccgc cccgagtggc gcgaacagct cgagccgttc ctggctacag catccgaaga
      781 gcacgccgag ggattgcggt ttcgccgcaa tcaggtgctg ctgctcgacg cgatgctcgc
      841 cacgcggaag aacgtcgcct tcgacgacgt gttcgaacag gcgcggcgca agatcctcga
      901 cgtcgtcgtg cagcctgagc agccgcctga ttcattcacc ggcgagttgc gtccctatca
      961 aagcgaaggc ctcggctggt tcacattcct ccgcagtctc agcttcggcg gctgtctcgc
     1021 cgacgacatg ggcctcggca agaccattca ggtgctcgcg ctactcgatc agcgccgcaa
     1081 cctgccgaag aaggagcatc gtccgtcgct cgtcgtcgtc ccgcgctccg tcgtcttcaa
     1141 ctggaagaag gaagcggcgc gtttcactcc gaaacttcgc gtgctcgatc acgccacgcc
     1201 cgaccgcatt cgcgccaccg atcacttcct cgaatacgac gtcgtgatca cgacctacgc
     1261 gcttctgcgc aacgaggtcg agctctttgc cgatttcgaa ttcgactacg cgattctcga
     1321 cgaggcgcag gcgatcaaga acgcgtcgag tcaggctgcg aaagcggcca cgctgcttcg
     1381 cgcccggcat cgtctggccc tcagcggtac gcccgtcgag aaccacctcg gcgagcttgc
     1441 cagtctcttc gagttcctca atcccggcat gctccgcgcg aagacgacgc gcgtgacgaa
     1501 gatgctgcgc gacgccggcg ggaagacggc gcaggcagcc gaggcgcgcg tgatgctggc
     1561 gcgcacggtg aggccgttca ttctgcgccg gacgaaagcg caggtggcca ccgagctgcc
     1621 cgaccgcgtc gagcagacgg tgtattgcga cctcgagccg aagcagcgaa agctctacga
     1681 cgagctgcgc gatttctatc gcgcctcact cctcggccgc gtcgcggaga aaggcatcgc
     1741 caaatcgaag atccacatcc tcgaagcgct gttgcgtatg cgccaggccg cctgtcatcc
     1801 cggccttgtc gacgcaaaac gaaccggcga aacctccgcc aagctggaca agctgttcga
     1861 agagctgccg atcctgatcg atgagcggca caaggttctc gtcttctcgc aattcacctc
     1921 gcttctcgcc atcgtgcaac gcgagatgcg gcagcgcggc atcaattacg cgtacatcga
     1981 cggaaagacg cgcaatcggg aagccgaggt gagccgcttt caggaagact ccgagatccc
     2041 ggtcttcctc atcagcctca aagccggcgg cctcggcctc aacctcaccg ccgccgacta
     2101 cgtcttcctc ctcgacccct ggtggaaccc cgccgtcgaa gcgcaagcca tcgaccgcgc
     2161 ccaccgcatc ggccagtcca gaaacgtgat ggcctaccgc ctcgtcgccc gcgacacgat
     2221 cgaagaaaag atcctgttgc tccaacaaag caaacgcgcg ttagccgagg cgatcatcgg
     2281 agaggagaac agcatcctgc gacagttgac ggcggaggat ctggatttgc tgctgtcgtg
     2341 agcctgcgcc ggtcggcctc aggacacgct cctgagaggt gattgaggga aacctgaggc
     2401 cttcctgaag actttgagag gctgcgtaac cgatcgtgga caccgaagga gatactccat
     2461 gcgcacgatc attcttgctc tcatcgttct ctcgctgacc tctgcccttt ctgcccagaa
     2521 cgttgtcgtc gggacgcaat cgctcggggg caccatcgcc cagagcgcgg ctccgccgtt
     2581 cacgatgatc gatctgtcgc acccggcgac ggcagacgga tcggtagcga gcgcttccgt
     2641 tcgttgggcc gcgggaagtt gcaccgccgc attcaaattg aagtttctgc ggccggccag
     2701 cccgagctcg ttgaccacgt tcactcttgt ggccgagcga gggccattca acgcggtagc
     2761 cgggaggaat cagcttgtgt tgacgccgcc cgtcgatgtc catgcgggcg acctgatcgc
     2821 cgtgacgtcg ctggggacct tcgcagcctg cggctctcct gcgaccctca tcgattccgg
     2881 cggtgccctc atgcggattt cgggcgacgt ctcgaccggc agcttcaatg ggttttacac
     2941 gcgcgatcgt tccctcggcg cgcgtgccac cgacaccacc gaagtactcg agggtgtcgt
     3001 gacggccgcg ggatcgctgg ccggcaactt cggttcgttc ttccgcacct cgattcaaat
     3061 cgccagtccg ggcggcggga cgtcgagcgg gacgcttgtc tttcatccag ccggcgttgc
     3121 tgcctcgccc atcgatcagg ccattcccta caccgcgacc agcggaaacg cgatctcgtt
     3181 tacggacatc gtgcagcaga tgggaaagac gggattgggc acgatcgacg taatctcgaa
     3241 caacggtttt ccgcccatcg tgacggcgcg gatttacaac gacacgccga atgggacatc
     3301 cggattcacc gaggagatga tcacgccgcg cgatgcactg cattttgggg acaccggagt
     3361 gctgctcacg ccaccggatc tgacgaagtt ccgcgtcaac atcggggtcc gcaccttctc
     3421 ctcgccggcg acgatcaacg tgcagtacgg ctttcgatcg cagagcaata tggactttcc
     3481 ggcgaacacc ttccggcaat actcgctggc cgaattcggc gacgcatcgc cgctcgcgaa
     3541 cgagcagatc atcctttcta tgatcagcgg cgatgcgatc gtgtacctgt cgacgaccga
     3601 caaccagacg aacgacagca gtgtgcgatt cgcacggcgg aactagcgct gatcgagtcc
     3661 gagtttggcc aggaagggag cgaagcgcgg atcgttgtgc agaggatcga gtctcggatc
     3721 gatgtgtact tcgaaaatga aagggtagtg cgaggccaga gagtcgtcga gggcgacgaa
     3781 ggctgcggcg ttgtcgccga gcgcggcgta gacggtggcg atctcataat cggtgcgcca
     3841 atcgccgtga gtgcgcaact ggcgcagcgt ctcgatctgc ttctgcgcgg tggcacgatc
     3901 acccgcgcgc gcagcgatta cgccgaggtt gctgacggcg cgcgcgtcat taggagcgat
     3961 ggcgagagcg gcactcgcca actgcgctgc ctggccacgc tgacctcggg agatcaaagc
     4021 gaatgccgtg ttgttcagcg caaaacggtt gcgtggctcc agcgaaatgg cacgccttcc
     4081 ctctgcgatg gactcgtcga agcggcgcgc aaagaacagc gacttgagca gatcgtttct
     4141 cgggacgatc cacgtcgggt cgatctcatg tgcgcggcgc acttcggcga tggcttcctc
     4201 cggacgcgcg aacgcctgca gcagataggc ccgcaagctg tgggcatcga catcgttcgg
     4261 atcggaagcg agcgcccggt gcagctcctc atccgcttcg cgccagttgt gtgtatagaa
     4321 gatgccgctg ccgcccaggg cggcatgtgc attggccagg tcaggatcga gcgccaacgc
     4381 gcgcagcgcc gcctcacgtg ccttcatgaa cgattcttca cgcggcaggg gaccgtagat
     4441 tccgatgacg gagtagctgt tcgccagtcc agcccacgcg tccgcatagc ggggatcgag
     4501 cctcagcgct gcctcgtagt agcggatgct cgaaagaaag ccagcctgat caaacatgtt
     4561 ccactgaaag cggccctgcg tgtagagctg ataggcctca gcgtttttcg tggagtgcct
     4621 ggcgaggtcg cggcggttac ggccactgag ccggaggtcg agcgcgtcgg cgacgcgttg
     4681 tgcgatccga tcttcgagca cgaagagatc ggacgcgtcc tcgtcgtatt cgtcggccag
     4741 gatcgcacgc ccatcgctga cgcgcaggag ccgtacgttg cagcggatgc gctgcccgac
     4801 gcgctggatg ctgccctcga cgacaccatc gacgtcgagc gtgcgccccg cggcgagcga
     4861 atcgacggga tgatcgacga aggcggccac agcgcgcgtc ggggcaatga tcagatcggg
     4921 caagacactg agtttcgtga tcagcgtgtc ggcgatgccg agctccagag gacggtcgcc
     4981 gcgctgctcg tcgagcgggc ggaacggaag gacggcgatg gagtggatgg cgtcgccctg
     5041 gtgccgtttc cgcagtttga tgatggcgat cgtcgccgct gcgatcagga tgactgcgat
     5101 tgccgcggcg atgacgagcc gcgaccgatt ccatcgtctg ttcggttccg acccctgcgc
     5161 ctccgccgga gccggcatcg cagcagaatc gtccgcgctc accttgcccg agaacctgta
     5221 gccgcgccgc ggcacggttg cgatccattg tccgtcgtcg tcgccgaggg ccttgcgcag
     5281 gacgaagatc gtctgggaga ggttggattc gtcgacgacg acgtcgggcc acacctgcgc
     5341 gagcaattcc tctttcgtcc gcagcgcgcc gggctccgcg gtgagcgcaa tcaacagatc
     5401 aaccgctttg ggaggaagcg ctgccggctg gccgtcgcgc ataagaaggc gctcccgacg
     5461 atcgagacgg aacggtccga agcggagata gcgcagctcg ctcatgcagt cgggcgcatg
     5521 cgcgggggcg atcccatcgt catcggttca ccaaacaagc gagcgatgga cgccggccac
     5581 tcgtgaacct gcgatgcgct gtcgtgttga tcgtgcacta ggagctcgtt gatcgagact
     5641 tgagcctttc ctgaagactt ttctctcgtc gcaaccgacc gtatgcgggt ggccgatggt
     5701 aacgcaagtg accgtccagg gaggttccga tgagacaagc tttctcgatc gatcaacgtc
     5761 gtgggaacgc gcgggcaatt gtcactaccc gccgcgcggc agttctcgtt gcactcgtcg
     5821 cacttggtgc ctgcaaccgc gtagcgccgt ccgccgcctc ggcgagcagg gttgcatcca
     5881 cgtccgttgt cgaaaacaac gactcgtccg cgccggcctt tgaagacaag gtccccgata
     5941 tcgaggcgtg cgcgcttctg gatcgcgtcg atgtcatccg gatcttcggt cctcttcgcg
     6001 atgaggcgaa gctcgatcgc ggccttcgcg gcgagaggga ctgccgctgg cacaacgagg
     6061 aagggcaatg gctgaaagcg agtctttacg gcgccagccg ctgggagttg gagaaggcaa
     6121 tcgtcagcga gatgcacccg cgtgcgattg ccaacgccgg agacgaagcc ttttccgtca
     6181 agcagggcac cgacagtgtc gttcacgttc gcaagggagg agcggtcatc gaagtgagct
     6241 gctcttgcgg catcgaaaaa tccgaatcgc tggcgcggat cgccgccgcg aggctctagg
     6301 tgagaaaaat cgccgtccgt ttagaagcga tcgagccagg ccagtacgtc gcgcgtcatt
     6361 ccgtcgaagc tttgggcgac gaagaggcgg tcctgatagt gcgttgggtc gtacggtgta
     6421 tcggcgacga cttcgacgtc gaaatccgtg agccactcct cggtctcgaa gcggccgagc
     6481 tctccgaatg aggagaggag gccggcgccg tagaccttga gtgctttctc ttcgcgcacg
     6541 acgccgaact cgagcgtgta ccagtagacg cggccgatcc gttcgatgtc gctggccgag
     6601 gcggtgagcg cggcgcggcc gaaggcgcga ttcagttggg cgaagtctgc gtggacgaac
     6661 gttgcagcat ggccgatgag ctcgtgcacg acgtccggct cgggcgtgta gagcgggacg
     6721 ctgtgatggc gcatgtactg cgtcgacaga aagacgccgc ggccgagctc tttgagaaag
     6781 gcctcggccg tcacgaggcc ggcgacgggg agcatgcgaa acccaaagtg cggcgcgatc
     6841 gtggtgttca cggcgcgaag ttgggggatc tgctggcggg acagggcgat gcgtgtggcg
     6901 gcatcgaggt actcgcggca tgcgtagcgc gcgtgaagcg agtcgagatg ccgccacact
     6961 tcgcgccaga cttcgtgctc cgcgtctgtg taaacaacat cgggcgcggg atcgccgtcc
     7021 cggtacgtga gcgcggcatg cgcgatctca tccctgcggc gccgatacga tggatcgtga
     7081 aatccggggt ggtccgcatc gagcttgacc agtgcggggg tgtgtgtcag gagcgccgag
     7141 tcagtcacgg ctctcacgaa cacgaggtgt acggaggcga ttcctcaccc gaaaacgctc
     7201 acgcgaagac gcgaaggcgc gaagaggaag gacgatgact gttgtcttcg cgtcttcgcg
     7261 ccttcgcgtg agcgccttcg cgtgagtctt cgcgcgatca cacctccagc cgatcgtgcg
     7321 ggcgcgcgac gagtgcgtag agcgcgggca tgaggaagac gctgatcaat aggcgggaga
     7381 agagacctcc gacgatgacc agcgcgaatg gtttttgcgt atcggatccg attccggttg
     7441 atagcgctgc gggcaggagc ccgagggcgg cgacgagggc ggtcatcatg atggggcgga
     7501 ggcggaggat tgcggcttcg cgcgtggctt cggcgatgcc gaggccgcgc tggcggagct
     7561 cgttcgcata agagatgaag atcacggccg tttggacgga cacgccgaat aaggcgatga
     7621 agccgattcc ggaggagaca gagaatggcg tgtgggtgag atagagcgcg agaaggccgc
     7681 cgatcgggcc ggagatcaga accgcggcga cggtaatcat cgggaacttg aagttcgaat
     7741 agagcatgaa gagcagcatg aagatcacga gcagagtcac aggcacgatg atctgaagct
     7801 gtttcttcga cgctgtgtaa ttctcgtatt cgccgcccca gacgacacga tagccggttg
     7861 ggacctgcac ggactttgcg acctggcgtt gtgcgtcttc gactgcgccc gcaagatcgc
     7921 ggtctttgat cgagtattga acgccgatgt agcgcgagtt gtcttcgcgg tagatgaacg
     7981 acgcgccgct gacttcgcgg acgtcggcca gctccttgag cggcacctgt tgaccgcctg
     8041 gtgtggccac gagaatgttg ccgatcgcct ccggagtctg gcggaactcc ggtttgaggc
     8101 ggaccaccag gtcgaagcta cgctctccct gaacgacctg tgtggcggca gtgccgccga
     8161 ccgctgcttc gatcagaccg ttgatgtcgg cgacgttgat gccgtatcgc gcgatcttgg
     8221 cgcggtccac cgtaacggtg agactcggtt gaccgagctg gcgaatgacc gagatttctc
     8281 cgatgccgcg aaccttttgc aggacggcct ttatcgcgtt tccacgatct tccaacacag
     8341 cgagatccgg tccgaaaatc ttcaccgcca acgcgctctt gagacccgtt tccgcttcgt
     8401 cgaccgcatc ctcagccggc tgcgtgaagt tgaagacgat gcctggaaat gccttgagtt
     8461 tttcgtcgat ggcttcaatc aactgcgcct tggagtgata acggccattc cactggctat
     8521 acggtttgag cccgacgaag aactcatcat tgaagaagcc ggtcggattg gtcccgtcgt
     8581 cggggcgccc caactcggag ctgacgacag tcacttcggg gaacgagtgc agaatcgtgc
     8641 gtacctgcgg cgagatcttg gcggcctctt cgaacgagat cgtcgacggc atgtttgcac
     8701 gcacccacaa ggagccttca tcgaggtgcg gcataaactc cgctccgatc cccgggatca
     8761 gcagcaagga gagtgcgaag agaatcacgc tgacggtcgt agtgagccag gggtgggcga
     8821 ggcaagcatc caggccacgc acataaacgc tgcggatggc ttcgaacacg acgttgcggc
     8881 gttcgcgcac cttgcgcaga gcccagaagc aaagagccgg taccagcgtc agcgacaaca
     8941 gcagcgcgcc gatgagcgcg aagatcgtcg tgtcggccat cggccggaac agtgcgccgg
     9001 atggaccggt caacgcgtaa atcggcagaa aggccgcaat gatgacggct actgcgtaga
     9061 agatcggccg gtctacttct gccgccgcct gcacgatgac ttcgctcgta gtgagaggat
     9121 caccatcctc gtgattgatc gccaatcgcc gaaaaatgtt ctcgaccatg acgacagcag
     9181 catcgacgag gatcccgaag tcgatcgctc cgatcgataa gaggttcgca ggaacgtgtt
     9241 tgaggtcgag acagatgaac gcgaacaaca gggacagagg gatcgtggcg gcaacgatca
     9301 atccggaacg gaagtcgtag aggaagaaga tgaggatgac gaggacgaga agcatccctc
     9361 gcaacaagtt gtcctcgaca gtgcgggtcg tgagagcgat caaatcgcgc cggtcgtaaa
     9421 acggacgaat cttgacgtcc ttcggcagga tcgagtcgtt cagctctttc gtcttcttct
     9481 cgacgcgatc gagcacgacc tgggcctgtt ccccagttcg catgaggatc acgccttcga
     9541 cggcctcatc gcgattgttg aagccgaact ggccgaggcg cggagcttcg ccgatggcga
     9601 cctgagcgac atccttgacc agcaccggcg tcccgttgtg aaccgccacg accacgttgc
     9661 cgatatcttc cggagtcgtg agccggccga gaccgcggac gtagaagaac tgcccgccct
     9721 gagagtagaa gccgcctccc gcgttgctgt tgttggcgct cagcgactcg acgatcagcg
     9781 ggacggacag cccgaccgcg gcgactttgg ccggatcgag gaggacttga tactgcattg
     9841 tcggcccgcc gaggccggag tcgtcggcga cgccgggaac ggacttgtat tgacgctcga
     9901 tcacccagtc ttcgagagtc ttcaactcca tcggcgaacg atcggggctg tcgaggacgt
     9961 agcggtaaat caggcccgac ggtgagaaga gcggcgcaat atcaggagcg acgccgtccg
    10021 gcagcgacac gccggcgagg cgctcgaata cctgctggcg ggcgaagtag ttgtcggttc
    10081 ccggttgaaa cgtgatgcgc acatcggaca gaccgtagag agagatcgag cgcacgacct
    10141 ccacgttcgg gatgccgttc atctctactt cgagcggaac ggtgatcagg cgctcgacct
    10201 cttccggcgc gcgcccttgc cattgcgcgg tgatctcgac ctgcggcggc gaaaggtcag
    10261 gatacgcatc caccggcagc cgcttgaacg agtagatccc ggcggcgagg agcagcgcgg
    10321 caacgaacag gagcagatag cgctgccgca gcgagccggc gacaacacgg ttgatgattg
    10381 aagttgctgt ccgctcgttt ggcgctaccg gcggccggat gtcgtcgatg tcgtccgaat
    10441 cgctcatgag tgttccaccg tcgagagaaa gagtccgccc tcgaccacga tcgtctggcc
    10501 cgcggtgaga ccgccggtga tctcctgctg atccccgatg cgcgatccga tctgcacgct
    10561 ctggcgcccg aacgacccat ccggtttggc gacgaagacg aacggaagat tctcctcatc
    10621 gcgcagcact gccgagacgg gaacgagcag gccctggctc tcctgctgcg actggatgac
    10681 cgcgcgaaca tagagatcgc gcttgagtac ctcgcccggg ttcggaacgt cgatgcgaac
    10741 ggagactgcg cgcgtattcg gatcgacgag cgcggcgatg tagctgactc tccctttcat
    10801 cggagccacg ccggcgcccg tcacgacgtc ggcggtgtcg ccgatcttca cgaagggaag
    10861 atcggactcg aacacgttgg cgagcaccca gacgttcgac agatcggcga ccgtaaaaca
    10921 gggtgtggtg ccggcctgca gcaactgtcc gggcgagatc agcttctcga cgaccgtgcc
    10981 ggcgagcggc gatcgaatcg tccctcccgg attcgtgacc ggtcggttct gctggatgtc
    11041 gtcgatcgtc tgctgcgtga cgccgaggga gcgaagctgt tgcaaggcgg aatcgcggtc
    11101 cgcctcggcg cccacggcat cggtctcggc ctgctccatg tcgcggcgcg cgatcgcatc
    11161 gttcttgaag agttggcgat cgagctcggc gacgcgcttg gcgttgcgcg cggtagcttc
    11221 cgcgcggcgc agtgcgctca tcgcggcggc aaagtcaggc gacgagacga tggccatcgc
    11281 ctcccctcgg cccacatgac tcccaaagcc gacgaggatg cgagcgaccg gccccgagat
    11341 gggagagagc acttgcgtgg agcggttctg atcgaaggca acggtgccgg tcgcttcgat
    11401 gctccggtgg aatcgcgaca cagccacgac ctcggtgcgc acgttgcgaa ccgccgtgct
    11461 cgcggtcttc atttcggttt cggtcttgct gttcgtttcc tgcgagcagg atgctacggt
    11521 caagaccagc acgacgcttg cgccgatcag ggcgatcgat tgcagggaac gattcgattg
    11581 ctgcttactc atgcgtttct cccgtgttca ccgtagacat cttgggaagc gacgtgccga
    11641 cggccagttc cagcgccgcc tcggcgtcat tggccgcacc cagggcgtcg gcgtactggc
    11701 cctcggcgtc gagcagggtt cgtttggcgt cgagcagatc gagcgccgac gagccgccca
    11761 atccgtagct cgcagaagcg acgcgataaa cctctttcgc ttccggcaaa agttgatcgc
    11821 gaatgaaaat cgcctgacga agcgcgatcg aagcgttagc gaatgcggtt tgcacgtcga
    11881 ggctgatctg cacgcggatg tcgctgatgt tcgcggtgag ctcggcctcg cgatgttggg
    11941 ccgcggcgat ctcaccgcgc tggtgttgcc aaaagaggat cgggatgccg aacgagactc
    12001 cgctggtgaa tgacgttggc gtcccctccg ccgcgttgcg cgtgaatgag acgctgacgt
    12061 ccggtgccca gaatttgccg gcgagccgcg tggcggcctt cgcgccttcg agctgcttct
    12121 ggaggctttg cagttcgggg cgggacgact ccgccagttt ctcgagagtc tcgaccgcag
    12181 caagcgagcc ggggaacgtc aggtcatcag tcagttccag cggcgcgccc ccggatcgac
    12241 ccagcagacg attgagcgtc gcgcgtgcag tagccgcggt gcgctcgttg gcgatgaggt
    12301 cgttttccgc ttgtgaaaca tcgacttgcg ccttgaccac atcgaccttg gcaacggtgc
    12361 cggccatgaa tcgcgcctgc gtcttctgga gaaagtcctg cgcgaattct ttgctcgtga
    12421 tcaagtcgtc ggcgtgccgt ttcgccacga ggattgcgtc gtaggcctgc accgcctgcg
    12481 acgccacctg ctggcgtagt tgcgccaggt tgaattcagc gacgcgcagg tccgcggtgg
    12541 cgacggcgcg tcgaaggctc gtttttccgg ggaacggaat cgtcaccgcc acgccttgat
    12601 cgctgccggt cgacgagcgc gggctcagcg gatgcggcag gccggatacg tccgcggcga
    12661 agctcggatc cggaaacgcc gctgcgacga cgacctgcgc gcgcgcttgc tcgacctgtt
    12721 cacgcgcggc gatcaggccg taattacgag ccagagcctg atcgatcgcc tgctgccgcg
    12781 agagccgaag cagttcgccg gcaggttgaa catcctgcgc atacgcgaat ggtgaaatac
    12841 cgccaacaac aaaaagaatg cagaagcccg cgctcttgag cacagagccc tccttgaacc
    12901 tggaaggtca agtggaggga aagggggaac tagcaggcgg gaggggcgcg gccgcgggcg
    12961 tcgtcgtgga atccggacgc cgcaaacaca gtcagggtgg gcagtgcgag agtctgattc
    13021 acgctcggag gcgtaacacc ggtccacgag atcagtgccg tcgccagact gaacgtacgg
    13081 caggccaggc aatcgttcgg cgccgcattc cggccggacg accgcgtcgt gagcgatagc
    13141 ccccgatgct gctgcaccgt tcccagcgct ccggcctgaa gatgaacatg gagctgcggc
    13201 gcggcggcat acatctccac cgcggcgaac accaccacca tcaatccggc caaccagcgc
    13261 cgagcacgcc acatgggcgg ggacactaac acaggccgct gaacagccga aagcggtggg
    13321 cattgcagaa ctcaaaacca ttaaccacag agacactgag aacacggaga cctctgtgta
    13381 ctcagtgcct ctgtggttaa tggtttttgg ccagtcctgt gcacttgttt tcatgcggag
    13441 gacacgcaaa tgccagagaa gcaaactcta cgacgcgcaa gaaaagacaa ggcggaaggg
    13501 aaggcgccgt cgacgcaggc gggcgagttc gttcgtgaag agatcgatca cattcgcgaa
    13561 ggaaagcatg gcgcgcggtc ggccaagcag gccatcgcca tcgggttgtc gaaagctcgc
    13621 cgcgccggtg tgaacttgcc gcccccggcg aaaggaacga ccagcgagga gacgcgccag
    13681 tctgcagcgc gggctctgaa gaacggacgc gctggtggga atcacaagac gtcggcgaaa
    13741 cgctcgaaag cgacgctgaa agcgttgaag cgcgagccac gcgctgccgg atcgcatctc
    13801 gcgctcgagc gtcaggcgct cagcagttcc cgcaaacgcg gacctgccgc aaggcagcgg
    13861 gccgcggaga aagcggtcga gacgaaagga ccggccggtc tccatcgcgc cgcggtcaaa
    13921 gcggcagcga caaggagccg tgcgcatcac tagtcgtgaa tcccgaggag aggaggtcaa
    13981 tgatgaaaaa ggttcgattg agctttccac agatgatgtt tgtgatcgcc acgcgcgcag
    14041 cactggccgg cggcgtcgct ctgctgaatg cagaccgggt caagagggag acgcgacgaa
    14101 ggatcggttg gattctggcg ggcgtcggcg tcgccaccac ggtccctgcc gcgatgatgg
    14161 cgcgccgcaa tctcgttgcg tgatgtgaca ctcagtggag gtacgtctgg aaccagtcgc
    14221 gcgccagctc ggcgactcgt tccagcgttc cccgctcttc gaacaggtgc gtcgcgcctg
    14281 gaatgatctc gatacgcttg accgcgctca tttgttcgaa agcgcgttgg ttcatgctga
    14341 tgaccggctc atcgttgcct ccgacgatca ggagcgtcgg cgccttcacg agtggcagtg
    14401 agtcgcccgc cagatcgggg cggccgccgc gcgagacgac agcggccacg cggtccggcc
    14461 tgcgcgccgc tgcgacgagc gctgcgcctc cccccgtgct ggcgccgaag agtccgaccg
    14521 gcagcgattt cgtttccggg ttttgactga gccactccac cgctcccgcc agccgtcccg
    14581 ccagcatgtt gatgtcgaac ctcaattgcc ccgtcctctc atcgacccgc tcctcctcgg
    14641 cggttagcag gtcgatcaac aacgtgccca gccctccttc gcgcagaaac gcagcgacgt
    14701 gccggttgcg cgtgctgaag cggctgcttc cgctgccgtg cgcgaagaga acgaggcccg
    14761 aggccgcatg agggatgagc agattgccgt cgagggaaac aggaccgatc gacaaatgaa
    14821 cgagtccttc gtcgtgagcc gtcatcgtgg acatggggtg ctcctcacga tgcggctccg
    14881 agcgtcttcc atgcccggtc gagcagtgcg tgaacctctt cgtcggtggt ctggccgaag
    14941 tcgtcgtacc agagcccgac cgcctggaac ggagacggtg tgcgcaggca gacgatgtcg
    15001 tcggcgatgg cgcgaaactc gaggcacgtc tcttttgcag cgacgggaac ggccacgatc
    15061 acttcgtgtg cgttcatatt gcgcagcgcc atgaccgcgg cgcgcatcgt cgagccggtg
    15121 gccagaccgt catcgacgac gaagaccgtc ttgccggcga cgtcgatcgg cggtctgact
    15181 ccccgatagc tcttctcgcg ccgctccagc tcctgttgct cggctttagc cacggtgtcg
    15241 atgacacgat cggaaatcga tgcgtaacgg atgacctcgc ggttgagcac gcgcacgccc
    15301 ccactggcga tggctcccat ggccagctct tcctgaccag ggacgccgag tttccggacg
    15361 atgaacacgt ccagcggtgc gttcagcgct tccgccactt cgaagccgac cggtacaccg
    15421 ccgcgcggaa gcgcgaggac gagagcatcg gcgcgatgtc cgaatcgccg gttcagttcg
    15481 gcgcccagga ggagtccggc ttcgacgcga tctcggaagg gttgatcgaa catcagattc
    15541 atcccttagc tttccttctg caagacgcgc gcatgttcgc tcacgcgaag acgcgaagac
    15601 gaacgggaag gaaagcccgc aaccgtcaaa cgcttcgcgc cttcgcgtct tcgcgtgaga
    15661 acctaacgag aagccagcgc gttcgctacg agctcgcgcc cagcagcacg atgtcgctcg
    15721 tcttgatgta atgcgtcaac acgatccggc tcccgtctgc ggaccattcg tagttctcga
    15781 tcgagccctc tgtgaagttc gtcagcgcgg ttggtggtcc gcctgccagc ggctgcataa
    15841 agagattctc ctgtttgccg tcgtgcttga tatagacgat tcccttgccg tcaggagtcc
    15901 agcgatagag atggccggag aggtcgaaga tccttgtcgg tgcgccgccg ttgatggaga
    15961 tgatggccag cttggggttg aacggcgact tccaggcgga ggcgatcagt tttccgtcgg
    16021 gcgagatgga gccatagttc gatgcttccg aggtcagccg ggtgaccggg ccgccggtca
    16081 ttgcgacttt gaacagcctg aaggcctggc cgtcgagcag cgcgtagatg accgtgcgtc
    16141 cgtcgggtgt gatctgcatg ggtttgagga tcgtacccgc cgtcgcaatc gtcgagggct
    16201 tgccgccgtc gacagggatg ctcatcagcg atgagaagct ggtggcgtcg ttgcctgatg
    16261 cgtagacgat ctgttttcga tcgggcgtga ggatgaagtc gtggatcacc ggagcactga
    16321 cgatctcgcg ctgctggccg ccgtcgatgt tcatccgcca gagagacgac cgtccctgac
    16381 tgtgggtcgc atagacgatc gtcgtgccgt tggcggtgac gacgggccgc gactcgagcc
    16441 gttcgtcgtt ggtcagttgg cgggcatcgg atccatccgc gttggcgatc cacagatccg
    16501 cggttccgcc tgcggcggac gcgtagatga tgctgcggtc gggcgcggtc gtcagaccgg
    16561 agaggccgtc gaagctgccg aggccgcgtg tcaactgcac gggggcggtg ccaggaacca
    16621 ccgaccagat cgcggactga tactttgcct gaatggccgc gaccgacttg ccgatcgcgt
    16681 gtggctcggc gtagtcatcc gagtcgtccg tcacgttggt gacggcgccg gtgccgagat
    16741 tgacgcgaag cagttggcag tgtccggccc agcggtcatc gagcgcggtc acgagcagcg
    16801 tgtcgcccgc actagtccag gccacgtcgc gaatcgcgcc ctcggatgta gccatgggga
    16861 tgctcgtctt ggcgccggtg ctgacgtcga tggtgaagag actatggccg tgcgaaatgg
    16921 cgatcctcgt tccgtccggc gaccacgcgg gcgagggcat catcatgcct tccgttagcg
    16981 gcagcgtcgc cagaacgcgc tcatgcgatc cgtcgggcgc cgtaagcagg agacggctct
    17041 cgcccttctc gagaacgttg cgcaccacgg ccagaagttt gccgtccggc gacggtgtga
    17101 cggccgtgtc agcgtcgccg atgatgcgcg ccggagtgcc gccgaggagc ggcaccttca
    17161 ccaccgcgcc gaagatcgtc ccttcgtagc gcgaggcaag gaggtgattc ccgtccgctg
    17221 aaaaggcgag accggtgtaa tccattttcg gcgatggcgg gagaacgacg acgcggctgc
    17281 cggtagcgat ctgctgcagc cacacggtcg ggccggcagt cccgtccgac aacgtgtagg
    17341 cggcgaatcg tccgtcgggt gaaatggcga gatgggtgac gtggccgttg gtcgtgattc
    17401 gttgtaatgc ggtatgcgca accggagcgg ccgtcgttgc cgaagcgctc atccgaagat
    17461 ggatctgcca gccgatgact ccgatgacca cggctgcggc gaaagcagcc agccagcgca
    17521 ggccgtgagc ggctttgggc tgctcgagtt ggggcagcgt aatgggtccg gtaggattgc
    17581 cggcgttcgc cgccgccgca cgcaggtcga gcgcgacgtc gcgaatcgac tggtagcggt
    17641 actcgcggtc tttctcgatg cacttgccga cgatgcgccg cagctcctgc tgcatcagtg
    17701 ggtcgtaatg ctcgatcgct ggaggcgtcg cgtgcagcac ttcgtgcagc gtgtcgacga
    17761 acgaatcgcc gttgaatggc ttcgcgccgg caatcgtctc gtacacgatg cagccgaagg
    17821 agaagatgtc ggatcgctga tcgatgacct tcccgagaat ctgttcgggc gacatgtagc
    17881 cggccgttcc gatcagttcg ccctgttggg tgagtgccct catcgtcggc gagtcggcac
    17941 cgatcggatt ccatcccgcc gttggctcca cgagtttcgc caacccgaag tcgacaacct
    18001 tcgcgtaccc ctcagtggac accatgatgt tgtccggttt gagatcgcga tgaacgatgc
    18061 cggccgcgtg cgccttgccg aggccctcgg cggtttgggc gaggatttcg aggcggcggc
    18121 tcagtgaacg gccgccggcg atgaactcgc gcaacgtctc gccttcgatc aactccatgg
    18181 cgatgtagtg gatctcttcc ggacgctgac gctgatccgt tcccggaatc ggctgcacga
    18241 tgcaggaggg acgagcctgg ccgatctcgt agatcgtgac gatgtgcgga tggttcagag
    18301 ccgaagccga tttcgcttcc tgcgcgaaac gctcgagacg gtcgcggtcg tcggtcaaac
    18361 cggtcggcag aaccttgagc gctacgctgc ggccgaggcg ctcatcgcgg gcgcggtaga
    18421 catcgcccat tccaccggac gcgagcaggg agaggatgcg gtacgggccg acattcgttc
    18481 catcgggaag cggcaggcgg ggtgattcct gaggaatcgc gactccaacc agaatcccgg
    18541 catcgaccga ttgcatcact cacctcctgt aagcagaccc gtattgagtt atacgcgggg
    18601 tttcggaaag gttcgtggac gagcggttac ggttcagagc cggaacggga aggaatgcgg
    18661 gcatcaatgg gtgcgacgcc gcggacgtgc tacagtcctt tgcgtggggc actatcgcgg
    18721 tgcctcatcg atggcgcgtt ggttacctgc ttgcgtcgtt gccgcaaggt tcgttgcgag
    18781 tcccccacgc cacgtctcgc gaggccatcc tcgtgctcct ggccgcggtc gaaacccgct
    18841 gaaaggcacc agatgtcctc atgttcgttt aaacccgcgg cggagctgcc gcaagcacgc
    18901 aattcgcgtc tcaccgacgc accgatcgtc tccagtctcg gccccgaacc atggaagagc
    18961 ccgtggtgga agaccgcgaa aggcgacggc gccacgttct attacatcgt cgctattcac
    19021 atcctcgccg cgattggtct cgttttcttc ccgacgccgg gctggaaggt gatcgtcgcg
    19081 tcggtcgcac tcggctggct cggaggattg ggaacgacgg tctgctacca ccgcgcgctg
    19141 gcgcacacgg cgcttcgcct tcatccggtg gtgaagcatg tgctcatctt cttcgccatg
    19201 ttcaacggct ccggatcgcc cgattcgtgg accgcgaatc acagacaaca tcattccaag
    19261 gtcgagacgg tggacgatat ctccagtcct gccatcggcg gcttctggtg ggcacacctt
    19321 cgctggctgt ggcaggcggg aaacgttccg ctgaatcgct ggtgccccga tctcaacaag
    19381 ccggagtatc gcttctggaa ccggatccag gttccgatgc tggccgtctc gctctttggc
    19441 ggtcttgtct tcggcctgcc cgcgtttttc tggctcggct cgatgcgcct cgttgcatct
    19501 cttcacgcac agtgcttcgt caacagcatc gcgcacatgc gcgagaatcg cgaacacggc
    19561 gaggattcat cgcagaacat gggatggctc gcgcctgtcc agttttttca aggtgagaac
    19621 tggcaccaga atcatcacgc ctcaccatcg tcggcgcgtt tcggatggaa gttctggcag
    19681 gtcgacgtcg gctggtacag catcttcatg ctcgaactgg tcggtctggc gacgtcggtg
    19741 aagcgtccgt cacatcgtga gaaggctgtc agctcggtgg ttgctcctca ggtggttgcc
    19801 cctcagctgg ttcctcctca ggtcgttgcg ttcgatacgc ctgcctgagc ggttcacgac
    19861 gctcgccccg actgcgtaag caggaatcgg agtacgatcc gatgagcagc cgaggaggac
    19921 gcattgaact gtccgcgtga tgccgcagag ctggaagtga agtcgttcgg tacgacgccg
    19981 gtccggactt gtcccgtttg tagcgggatg ttcctggcgc atggacagtt gaaccaggtg
    20041 gccggcccga tccgcggcga cctcgagttc tgcacggtcg atctcgactc gtttcagcac
    20101 gaggacgcgt acggaccgat tacctgcccg aacgacggcg cgccgatggg caaggtcgac
    20161 ttcaacatcg acaccacgat catcctcgac tactgcaaga agtgcctcgg tttctggatg
    20221 gacggcaaag agctggcgcg catcaacgaa gaagtgaaac gcctgaacga agcggatgcc
    20281 gaagtccccg acccgctgct cgttcgcatc tcgcagttct tctggaacct gccactaccg
    20341 cactagcgtc tcatctgcgt tcatctgcgt aatctgcgga tccgcctcca cagagggaag
    20401 tcccgaaagc catcgcagac gcacaggtgt aagctattcc tccacaatca aaggagggag
    20461 aagagtatga gaacgacacg gttcctgccg aagatcgcgg gttatgcttt cgcttcacta
    20521 ctttgtgcgc tgctggcgtt catggccggc tgcaaagctg agacggcaac caataccgat
    20581 acctctgcca cgacgaccgc ggctgcttcg acgaccgaaa ctgcgtcgac ggccgccacg
    20641 tccagcgccg ctgcggcggc cagcggcatg tccaccttca aaccggtgcc gctgcctgat
    20701 ccgcacatcg ccggctatgc gttccctgag cccgagccga cgatcatcga ctggacgaag
    20761 accaacaacc agaaagcgat caacacgcat gcgtggggca tctggacggc gttgacgcag
    20821 gagacgaccg agaccttcgc gaatcagaag ctccgtgtgt tcgagacatg gccggacgtg
    20881 gacgacctga tggctgcgtc ggcggcgcca gccggaggga tagcggcaat ggcgacgacg
    20941 ccgcgtctcg cacaccggct gaaaaagccg cgtcagttcg agcacggcgg aggcgcggcc
    21001 gcgctcgggc tcgccgccaa cccgggcgaa tcgaccgttc tcgcgttcgt gaaatatgac
    21061 cccatcggcg gcgatcacat cgtcaagaac aagcttttct cggcatcgaa tctgagcgcg
    21121 atgctcgctg ccggcaagca ggccatcccc gattttccga atccgtccct cgcgctcaag
    21181 ccggtattcc agacgctttc cggaagcacg gtcggcggcg gacgctacta ccggttgtcg
    21241 acctggcccg gctcgcccgc gctcacgtac aacagcaagg acaagatgta tcactcgatt
    21301 ccgtttccgc agaacctgtg gaagcaatgc gtctggatcg acatccaggc gacgggcaag
    21361 ggacctggga ctggcggcgt agacacgacg tgcgccgcgg acggctcctc gcggacaccg
    21421 gccagcacgt acagcgtcga tgagttcatc cacttccagt tgacggcggc cgaggccgcg
    21481 gcggaaaacg cggaaggtca ggtgccgccg gcgaaggcgg gcgatttctc cgtgctcgtg
    21541 gcgatgcacg tcacctcgcg cgagatcaca cgctggacgt gggagacgtt ctggtgggtc
    21601 ccgaaccccg acaaaccaac ggcgccgagc tcgccggcca tcgccgccga tcgcccgccg
    21661 caactccagg gcgcgccgcg caactacgcg cactgcactg cctacgacat ggagaacccg
    21721 gccacgccgc tcgtcggcgg caacaacaac ggagactcca tctactgctt caatccgtat
    21781 ctcgaagcgc cattcactcc cagcgtgctg cccaaatcga ttccgggcaa caccatgttc
    21841 cagggcaaga cggtgcagac cgccaacaac gtcggcgcac aaacgaactg catgagctgc
    21901 cacggacaag cgaactggaa cccgccgacg cagccgaaca tctacacggg cgatcagtac
    21961 atcgccatga atgcgccgca attcaaaggc gtgctcaaag ccgacttcct ctggtcgatc
    22021 cccgacaacg cgaagtagtt cctggcgccg gccgtgatgt cacggccggc gtttgtttct
    22081 tgggggagtg gggggtgggg agtgggg
//
LOCUS       DATLVW010000037         3960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_456695, whole genome shotgun sequence.
ACCESSION   DATLVW010000037 DATLVW010000000
VERSION     DATLVW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456695"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VNN25_01365"
     CDS             complement(<1..1629)
                     /locus_tag="VNN25_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXH90199.1"
                     /translation="MSLRALLLRHRKDPRFIVTLPLLLLAATSLIYYAVQRAKELSTE
                     ALSSQMLLFVLANINVLLIFGILFVLLRGVVKLVLERQRGILGSRFRTKLAVAWVATS
                     LIPVLILFFFATDLLRTSIDHWFNAPVSRILTNGETIAQMAQDQSASMAAAAAREIAF
                     SSKVDDPAQLEGVLSHVQQFHGVDMVGLYRDGELVRVVANPRAPVQEVSEPPRKFFDD
                     VLAKGQATKIDVGASGKWIRVATRVGSTPYVAIAGVFIPASMSRLIDESIIAHKTFEQ
                     LNAERPTLKASQTSLFLAVTLAVLFGTLWTSIYASRRITVPIKALAEATGRLAEGGYG
                     HRVAVAATDEVGRLIESFNEMSTQIDEQRQALTQANRYLSTVLDSVSAGILAFSDNFD
                     LLSINHAALQMLQVDEPADSQPRANLADVLTGDLTPLLETLRELTARGGRAREITLIR
                     GGELRYLEVSVARLSGSGDEGWVVAIEDSTSLMQAQKLAAWNEAARRIAHEIKNPLTP
                     IQLSAERIARKFRNGDHDTALAIDEGTKTIVSEVA"
     gene            complement(1626..2174)
                     /locus_tag="VNN25_01370"
     CDS             complement(1626..2174)
                     /locus_tag="VNN25_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXH90200.1"
                     /translation="MKSFPLTALLLLLASAGFSADPKIDTLTARSVNRQVNVHFTMGH
                     AFDRQQTVQGLQSGVPTSLVYIVEAYRDRPNWFDEGIARSRIEVIATFNSLTREYLLN
                     YRRDHKLVRSETFNDLASLEKAMTNIDEPALFDIGKRPPYKVMVRVKADLMRGWLLYF
                     IPWEISTRWMAVRVTAPPENGR"
     gene            complement(2225..3103)
                     /gene="lpxC"
                     /locus_tag="VNN25_01375"
     CDS             complement(2225..3103)
                     /gene="lpxC"
                     /locus_tag="VNN25_01375"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXH90201.1"
                     /translation="MHVKRKSISTKVSITGIGLHSGIYTKVELHPAHAGSGITFVRAD
                     LGMRIPALQASTTALDHATTVGRDDVSVGTVEHLLSALMACGITDLDIHINGPEVPII
                     DGSALPFMHLIDAAGVRELGADVRVLRVKEPIEVVDGNRSIRITPSNRLVVKYKIDFD
                     HPTIGKQALQFDFHHDSFLKKIAPARTFGFKQDVDKLRAAGLARGGSIENCIVLDERG
                     VMNGPLRYRDEFVRHKILDLIGDLALIGRPVVGEITAYRAGHALHSRFVEKILRAAEA
                     EEDGVLIGAAAAESFA"
     gene            complement(3274..3753)
                     /gene="smpB"
                     /locus_tag="VNN25_01380"
     CDS             complement(3274..3753)
                     /gene="smpB"
                     /locus_tag="VNN25_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXH90202.1"
                     /translation="MRGPMATKSEAGEKLIASNKKAYHDYFILQKYEAGLSLRGTEVK
                     SLRDGKANLKDAYVLFKGGEAFLFNANISPYTHGNLQNHEPERSRKLLLHRKEIEKAR
                     EQVVEKGLTVVPLRLYFKGGRIKVEIAVVRGKKLYDKRETEKRKEAVREAAVAMKQR"
     gene            complement(3755..>3960)
                     /locus_tag="VNN25_01385"
     CDS             complement(3755..>3960)
                     /locus_tag="VNN25_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH90203.1"
                     /translation="FAAALDLPINRYSHKPLLPRILALRENVTAYDGAYVALAEILDL
                     PLVTRDGRLARSSGHTARIEFIE"
BASE COUNT          714 a         1192 c         1289 g          765 t
ORIGIN      
        1 tgcaacctcg gagacgatgg tcttcgtccc ctcatcgatc gccagcgccg tgtcgtgatc
       61 gccgttgcga aactttcgcg cgatccgctc ggcggagagc tggatcggcg tcagcggatt
      121 cttgatctcg tgcgcgatac ggcgcgcggc ctcgttccag gctgcgagct tctgagcctg
      181 catgagcgac gtcgaatcct cgatcgccac cacccaacct tcatcgccgc tgcccgagag
      241 gcgggccacc gacacttcga gatagcgaag ctcgccgccg cggatgagcg tgatctcgcg
      301 cgcccgccct ccccgcgccg tcagctcgcg cagcgtttcg agcagcggcg tcaggtcgcc
      361 ggtcaggaca tcagcgaggt tcgcgcgcgg ctgcgaatcg gcaggctcgt cgacctgcag
      421 catctgcagc gcggcgtgat tgatgctcaa aaggtcgaaa ttgtcgctga aggcaaggat
      481 tccggccgag acgctgtcga gcaccgtcga gaggtatcga ttcgcctgcg tcagcgcctg
      541 ccgctgttcg tcgatctgcg tcgacatctc gttgaacgat tcgatgagtc tccccacttc
      601 gtcggtcgcc gcaacggcta cgcggtgtcc atacccgccc tccgccaacc ttcccgtggc
      661 ctcggcgagc gctttgatcg ggaccgtgat ccggcgcgag gcgtagatcg acgtccacag
      721 cgttccgaaa agaacggcca gcgtgacggc caggaaaagc gatgtctgcg aagccttcaa
      781 cgtcggacgc tcggcgttga gctgctcgaa ggtcttgtgc gcgatgatcg actcgtcgat
      841 gagccggctc atcgatgccg ggatgaagac tcccgcgatg gcgacgtacg gcgtcgatcc
      901 gacgcgcgta gcgacgcgaa tccacttccc gctggcgccg acatcgatct ttgtggcctg
      961 tcctttggcc agaacgtcgt cgaagaattt gcgcggcggc tcggacactt cctgcacggg
     1021 cgcacgcggg ttggcgacga ctcgcaccag ctcaccgtcg cggtagagac cgaccatatc
     1081 cacgccatga aactgctgta cgtggctgag aacgccttcg agctgtgccg gatcgtccac
     1141 cttcgagctg aacgcgatct cgcgcgctgc cgccgccgcc atcgatgccg actggtcctg
     1201 cgccatctgg gcgatggtct cgccgttcgt cagaatgcgg ctcacaggcg cgttgaacca
     1261 atggtcgatc gacgttcgga gcaggtcggt cgcgaagaaa aacaaaatga ggaccgggat
     1321 cagcgacgtg gccacccagg caaccgccag cttcgtccgg aagcgtgagc cgaggatgcc
     1381 gcgctggcgc tcgaggacga gcttgacgac gccgcgcaga agcacgaaga ggattccgaa
     1441 gatcaacagg acgttgatgt tcgccagtac gaagagcagc atctggctgc tcagcgcctc
     1501 ggtggagagc tccttcgcgc gctggaccgc gtagtagatg agactcgttg ccgccagcag
     1561 cagcaacggc aacgtcacga tgaagcgcgg gtcctttcga tgccgcagaa ggagcgcgcg
     1621 aagactcatc gcccgttctc cggtggcgcg gtcacgcgca cggccatcca gcgcgtcgag
     1681 atctcccacg ggatgaagta gaggagccag ccgcgcatca ggtcggcctt gacccgcacc
     1741 atgactttgt agggtggccg tttgccgata tcaaagaggg ccggctcgtc gatgttcgtc
     1801 atcgcctttt ccagcgatgc gagatcgttg aacgtctccg aacggacgag cttgtgatcg
     1861 cgccggtagt tgaggaggta ttcgcgggtc agcgagttga aggtggcgat cacctcgatg
     1921 cgcgatctgg cgatcccctc gtcgaaccag tttggccggt cgcggtaggc ctcgacgatg
     1981 tacacgaggc tcgtcggcac gccactctga agcccctgga cggtctgctg gcgatcgaac
     2041 gcgtgcccca tcgtgaagtg aacgttgacc tgccgattca cggagcgggc tgtgagcgtg
     2101 tcaatcttcg gatcagcgga gaatccggcg ctggcgagga gcaggagtag cgcggtgaga
     2161 ggaaacgatt tcacggtgtg gcaaatttac cacacagcga ccaatcgcac ggtgttgtct
     2221 cggtttacgc gaagctctct gccgccgcag cgccgatcaa aacgccgtct tcctcggctt
     2281 cggccgcgcg cagaatcttc tccacgaagc gcgaatgcag cgcgtgcccg gcgcgatagg
     2341 cggtgatctc gcccaccacc ggacggccga tcagcgccag gtcgccgatc agatcgagga
     2401 tcttgtggcg gacaaactcg tcgcgatagc gaagcggccc attcatgacg ccgcgctcgt
     2461 cgagaacgat gcagttctcg atagagccgc cgcgggccag accagcagcg cgcagcttgt
     2521 ccacatcctg cttgaacccg aaggtccgcg ccggagcgat cttcttgaga aagctgtcgt
     2581 ggtgaaagtc gaactggagg gcctgcttgc cgatcgtggg gtggtcgaag tcgattttgt
     2641 acttcacgac cagccggttc gacggggtga tgcggatgct ccggttgccg tcgacaacct
     2701 cgatcggttc cttgacgcgg agaacgcgga catcggcgcc cagctcgcga acgcccgccg
     2761 catcgatgag gtgcatgaac ggcagcgcgc ttccgtcgat gatcgggact tccgggccat
     2821 tgatgtggat gtcgaggtcg gtgatgccgc acgccatgag cgcggagaga agatgctcga
     2881 cggtgccgac ggagacatca tcgcggccga ccgtcgtggc atgatcgagc gcggtggtcg
     2941 atgcctgcag ggcgggaatg cgcatgccga ggtcggcgcg gacgaaggtg atgccactgc
     3001 cggcatgagc tgggtgaagc tcgactttcg tgtagatgcc ggagtggagg ccgatgccgg
     3061 tgatgctgac ttttgtgctg atcgacttac gtttgacgtg catgtccggg cctccaagga
     3121 gcaggcgagg tgccacgcac gtgcgatccc atcgaaatgc acgtaagctt tctgttcgca
     3181 gtcgtttacc ggcaaactta gctccggttt gtttccgcga ttctgtggtg ggtctgagtc
     3241 actagtgtgg ctgtctgacc acacgacccg gtctcaacgc tgcttcatcg cgacggcagc
     3301 ttcgcggacc gcttctttgc gtttctcggt ctcgcgcttg tcgtagagct tcttgccacg
     3361 cacgacggcg atctcgactt tgatcctgcc gcctttgaaa tagaggcgga gaggaacgac
     3421 cgtgaggccc ttctcgacga cctgctcccg cgccttctcg atctccttcc gatgcaagag
     3481 gagctttcgc gaccgttcgg gctcgtggtt ctgcaggttt ccgtgcgtgt agggagagat
     3541 gttggcgttg aagagaaagg cctcccctcc cttgaacagg acgtaggcgt ccttcagatt
     3601 cgctttcccg tcacgcagcg acttgacctc ggtgcccctg agcgagagtc cggcctcgta
     3661 cttctggagg atgaagtagt cgtgatacgc cttcttgttg gaggcgatga gcttctcgcc
     3721 agcttcggac ttggtggcca tggggccgcg gattctactc gatgaactcg atgcgggcgg
     3781 tgtggcctga ggagcgcgcg aggcgaccgt cacgagtgac gagtgggaga tccagaatct
     3841 ctgcgagcgc aacgtaggca ccgtcgtaag cagtcacgtt ctcgcgcagg gcgagaattc
     3901 gagggagcag cggcttgtgt gagtagcggt tgataggcag gtcgagcgcc gcagcgaagg
//
LOCUS       DATLVW010000038         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_580556, whole genome shotgun sequence.
ACCESSION   DATLVW010000038 DATLVW010000000
VERSION     DATLVW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580556"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..690
                     /locus_tag="VNN25_01390"
     CDS             55..690
                     /locus_tag="VNN25_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90204.1"
                     /translation="MSLLMKQGGPSRGALPLAGNEVLVGPDPDICKVTGVMFAARREF
                     LIKDLGERTYYSILTTLTPTTLQQAMNPVASAWYDFSTIVEYDKAIYQSCRAKYPHIL
                     ELVGAASAELGIGRVFHQLDAAELYAFLENLAQFHEQYQKYGQLTLERTPTGARMHYR
                     DYPCYSPYFCASGNGFMLEAILRHGATEADVVETQCHCRGDQVCTYELTWR"
     gene            complement(700..933)
                     /locus_tag="VNN25_01395"
     CDS             complement(700..933)
                     /locus_tag="VNN25_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90205.1"
                     /translation="MSKQAKHFAVCIRNDEYEGLLQLRKIYEVLEDAFAEQHNMIRVI
                     DEEGEDYLYPRDWFLPIELPQRVEEAIGGLTQS"
     gene            complement(935..1168)
                     /locus_tag="VNN25_01400"
     CDS             complement(935..1168)
                     /locus_tag="VNN25_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90206.1"
                     /translation="MKFELLTTISNVETIASGRGIRILDYLVRQFGRGHWFKRKGTAS
                     VLFEDGTIAHVELHWFEAHGIGKRKMRVKRGLK"
     gene            complement(1359..1733)
                     /locus_tag="VNN25_01405"
     CDS             complement(1359..1733)
                     /locus_tag="VNN25_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90207.1"
                     /translation="MRLEHLTAEEEDFWRGEFAQQNWIFGALGLSVTLPEFSGGTPAL
                     RKMHFGYYLQPLHLNADDREDLPRKLVHVLARRKPPLDFLELSGAGVRIPHCFPQGFT
                     LGSVLRITLQEMQKLPLKDEGG"
     gene            complement(1733..2221)
                     /locus_tag="VNN25_01410"
     CDS             complement(1733..2221)
                     /locus_tag="VNN25_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXH90208.1"
                     /translation="MARTNLTFDRTKQRALPIWLLILAVLLIVARIVSKQYPEKAEHD
                     LVRWMPISQAIGIARTTHRPVVYEFSAAWCGPCKMLEREVFMDEDLAKRINDRFIAVK
                     VVDTKQEEGRNWPDVQALMDRYAVNAFPTIVIAGPDGNVLDRVVGYPGAGKMAAMIDR
                     PR"
     gene            complement(2208..2945)
                     /locus_tag="VNN25_01415"
     CDS             complement(2208..2945)
                     /locus_tag="VNN25_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXH90209.1"
                     /translation="MSLVVVIESIRRHFAHLGYIAALLILIVIVVMMTAIGAPASAPY
                     ALISLFTLVAGCQLIGPEFSSGTLQLILSKPVGRSSYLTGRVAGVVLSIWVAVAIILA
                     SDIIGRLIAGTPIVWRTVLWTALVSALKVLLTCSLLALFGSFTRSYVNIGIYLGGQIV
                     LSGIYGLLSIMKNAFSGEMAAVGRLLRAHPGILEGYRAVQTNLFPDDPMIPFDRNWIL
                     LMTCNAVVAVLLACVIFSRREVPYGAD"
     gene            complement(2942..>3767)
                     /locus_tag="VNN25_01420"
     CDS             complement(2942..>3767)
                     /locus_tag="VNN25_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH90210.1"
                     /translation="KGVDLTIAPGEVVGLIGPNGAGKTTLMSCIVGFLNADEGTITID
                     GRSNDDLDVRRRTGFVPERMNFDRRANGGAFLRYMARLAGVPRDRVHARVDELLNRLG
                     LADAEDKRLSQYSRGMLQRIGMAQALMLDPDFLFLDEPTSGLDPNGVFLVRDLIAEEK
                     ARGAAVLLNSHQLAEIEKVCDRVLFLSGGVIARDEALREIARITIVVTMLPGSYDAAA
                     VEAAAGAVPVNDVVATNVTSEAEVAQLVSRIVSTGAGVVDVRRRTADLESIFRGEA"
BASE COUNT          760 a         1175 c         1142 g          690 t
ORIGIN      
        1 cactgtgccg aaagtcctat ctcccataaa atagagcacg gaagccccag gcccatgtca
       61 ctcctcatga agcagggtgg accctcccgc ggcgcgttgc cgctcgcggg gaacgaggtc
      121 ctcgtcggtc cagatccgga catctgcaag gtcaccggcg tgatgttcgc cgcgcggcgc
      181 gagttcctga tcaaggacct cggcgagcgg acttattact cgatcctgac cacgctcact
      241 ccgaccacgc tccagcaagc gatgaatccc gtcgcctcgg cgtggtacga cttctccacg
      301 atcgtcgagt acgacaaagc gatctaccag tcctgccgcg cgaaatatcc ccacatcctg
      361 gagctcgtcg gcgccgcctc ggccgaactg ggaatcggaa gagtctttca ccagctcgac
      421 gccgccgagc tctacgcgtt cctggagaac ctcgcgcagt tccacgagca ataccagaag
      481 tacggccagc tcacgcttga gcgcaccccg accggcgcgc gcatgcacta ccgcgactac
      541 ccctgctaca gcccctactt ctgcgccagc ggcaacggct tcatgctcga agcgatcctc
      601 cgccacggcg ccaccgaggc cgatgtggtg gaaacgcaat gccactgccg cggcgatcag
      661 gtgtgtacgt atgagttgac gtggcggtga ggtcgccgct cacgactgcg taagcccgcc
      721 aatcgcctct tcaacgcgct gcggaagttc gatcggcaaa aaccaatccc tcggatagag
      781 atagtcctcg ccctcttcgt cgatcacgcg gatcatgttg tgctgctcgg cgaacgcgtc
      841 ttccagaacc tcgtagatct tccgaagctg gagcaacccc tcgtactcgt cgttgcggat
      901 acagaccgcg aagtgctttg cttgtttact catgtcattt caaaccgcgc ttgactctca
      961 ttttccgctt gccgatgccg tgcgcttcaa accaatgcaa ctcaacgtgg gcaatcgtac
     1021 catcctcaaa cagaactgag gccgttccct tgcgtttgaa ccagtggcct cttccgaatt
     1081 gccgaacgag gtaatcgagg atgcggattc cacgcccgga ggcgatggtt tcaacgttcg
     1141 agatggtggt gagaagttcg aacttcacgc caccgaggat ccctcggacc atgtgctcca
     1201 accgaggaat ccgcttcagg tagagagaac cggtttgcaa acccctcgga atggaaaaag
     1261 cgtctaccgc acgtagagtc aaaaccttta ccacagagac acagaggtca cagagtaaga
     1321 cgatgttctc tgtgtctctg tggttcaaaa gaagtcgcct acccgccctc atccttcaac
     1381 ggtagctttt gcatctcctg caacgtaatc cgcagcacgc tgccgagggt gaagccttga
     1441 gggaaacagt gggggatgcg gacgccggcg ccgcttagtt cgaggaagtc gagtgggggt
     1501 ttgcggcggg ccaggacgtg gacgagtttg cgggggaggt cttcgcggtc gtcggcgttg
     1561 aggtggagcg gttggaggta gtagccgaag tgcattttgc ggagggcggg cgtgccgccg
     1621 gagaattcgg ggagggtgac gctcagtcct agggcgccga atatccagtt ctgctgggcg
     1681 aattcgccgc gccagaaatc ttcctcttct gccgttaggt gttcgagacg catcagcggg
     1741 gacgatcgat catggcggcc attttgccgg cgccgggata gccgacgacg cggtcgagga
     1801 cgttgccgtc gggaccggca atcacaatcg tcggaaaggc gttgacggcg tagcgatcca
     1861 tcagcgcctg cacgtcgggc cagttgcggc cctcttcctg ctttgtgtcg accactttca
     1921 cggcgatgaa gcggtcattg atccgcttcg cgaggtcctc gtccatgaac acttcgcgct
     1981 cgagcatttt gcacggcccg caccatgcgg cggagaactc gtaaaccacc ggccggtgcg
     2041 tcgttctcgc gattccaatc gcttgactga tcggcatcca gcgcaccaga tcgtgttccg
     2101 ccttctccgg atattgcttc gaaacaatgc gcgccacgat cagcaatacg gccaggatca
     2161 gcagccatat cggcagcgcg cgttgtttcg tgcggtcgaa ggtgagatta gtccgcgcca
     2221 tacggcacct cccgccgcga gaagatcacg cacgccagca gcacggcgac gaccgcgttg
     2281 cacgtcatca gcagaatcca gttgcggtcg aacggaatca tcgggtcgtc cggaaagaga
     2341 ttcgtctgga cagctcggta gccctccaga ataccgggat gggcgcgcag cagccggcct
     2401 accgcggcca tctctccgct gaaggcgttc ttcatgatcg agaggaggcc gtagatgccg
     2461 gagagcacga tctgcccgcc gaggtagatg ccgatgttga cgtaggatcg ggtgaagctg
     2521 ccgaagagcg cgagcagcga acaggtcagc agcaccttca gcgcggacac gagcgctgtc
     2581 cagagaacgg tgcgccagac gatcggcgtt ccggcgatca gacgcccgat gatgtcgctg
     2641 gcaagaatga tggcgacggc gacccagatc gaaagcacca cgcccgccac gcggccggtc
     2701 agatacgacg aacgtccgac cggcttgctg agaatgagct gcagcgtgcc ggacgaaaac
     2761 tcggggccga tgagctggca gccggcgacg agcgtgaaga gactgatcaa tgcatacggc
     2821 gcactggccg gcgctccgat cgccgtcatc atcacgacga tgacgatgag gatcagcagc
     2881 gcggcgatgt agccgaggtg cgcgaagtgg cgccggatcg attcgatgac gacgaccagg
     2941 ctcacgcttc acctcggaag atcgactcga gatcggccgt tcgccggcga acgtcgacga
     3001 cacctgcacc tgtgctgaca atcctcgata caagttgggc gacttcagcc tcggatgtca
     3061 cattcgtcgc aacgacatcg ttcaccggca ctgcgccggc cgctgcttcg accgcggcag
     3121 cgtcgtacga acccggcagc atcgtgacga cgatggtgat gcgcgcgatc tcgcgcagcg
     3181 cttcgtcgcg tgcgatcacg ccgccgctga ggaaaagcac gcgatcgcat actttctcga
     3241 tctcggcgag ctggtgcgag ttgagcagca ccgccgctcc cctcgccttc tcctcggcga
     3301 tcaggtcgcg tacgagaaag acgccgttgg gatcgagtcc cgaggtgggc tcatcgagaa
     3361 agagaaaatc aggatcgagc atcagcgcct gagccatacc gatccgttgc agcatgccgc
     3421 gcgagtattg cgagagacgc ttgtcctcgg cgtcggcaag cccgaggcgg ttgagcaact
     3481 catcgacacg cgcgtgcacg cgatcgcgag gcacgcccgc tagccgcgcc atgtagcgca
     3541 ggaacgcgcc gccgttggcg cgacggtcga agttcatccg ctcgggcacg aatcccgtgc
     3601 ggcggcgaac atcgagatca tcgttcgacc gtccatcgat cgtgatcgtc ccttcatccg
     3661 cattcagaaa gccgacgatg cacgacatga gcgtggtctt gccggcgccg ttcggtccga
     3721 tgaggccgac cacctcgccg ggcgcgatcg tcaggtcgac gcctttg
//
LOCUS       DATLVW010000039         3899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_249582, whole genome shotgun sequence.
ACCESSION   DATLVW010000039 DATLVW010000000
VERSION     DATLVW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249582"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1396
                     /locus_tag="VNN25_01425"
     CDS             65..1396
                     /locus_tag="VNN25_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXH90211.1"
                     /translation="MRRAWLVPRNLTLALALLLLTTCSSQTSDHRERLEFWALGSEGE
                     AVASFIPEFERRNPDIHVVVQQIPWSAAHEKLVTAFVGDAMPDISHMGNTWMPEFVAL
                     NALDDLGPYVASSAVVKQSDYFPGIWATNVVGATLYGIPWYVDTRVLFYRSDVLAEVG
                     FPHGPRTWAEWRDAMQRIHDQKKCRWAILLPTNEYEPITMLALSNHATLLTPDGTRGA
                     FSEPGFAGAFAFYVDTFRRGWAPAVSNSEIANLYQQFAEGDFAMYITGPWNVGEFRKR
                     LPPEMQDKWATAPLPAVNASEPMGISMAGGGSLVVFRASRHKAAARKLIDFLSEPAQQ
                     VRFYELVGDLPARRAAWQSPLLANDKHFPAFRAQLERVTPIPKVPEWEQIATAIYDRG
                     EAAARGAVPPAVALRQLDARADELLEKRRWMLAQSRSRGAAATQSRVGESP"
     gene            1393..2268
                     /locus_tag="VNN25_01430"
     CDS             1393..2268
                     /locus_tag="VNN25_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXH90212.1"
                     /translation="MKTPEARAALLFLAPAIVLLSVFFFAPVIAGFALSVTDFDLYSI
                     GDIHNLRFIALRNYGDLLGNPIFWTAFANTLYFALVGGPLTVAISLVAALLVNAKLTR
                     WKALFRTIYFAPVVTTLVAVSIVFKYLYHPRFGIINLALGAMHLPQPDWLGDPHWAMP
                     AIILLGVWKGFGYTMIIFIAGLQNVPEELYEAARLDGAGMWRQFRHVTLPMLGPTFLF
                     VGVVVAIGQLQIFAEPYVMTKGGPLNKTLTIVMMMYEQGFKFWRMGYAAAVAFVLFLI
                     IGAATLVQMRLREKQ"
     gene            2265..3086
                     /locus_tag="VNN25_01435"
     CDS             2265..3086
                     /locus_tag="VNN25_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXH90213.1"
                     /translation="MSRRSESLLLHSVLIAGGFLTLFPLLWMLSASFMSNGESTTFPP
                     HLLPHAATLGQYRQLFLRLNIGRAFLNSAIVASTVTISSVLFGSMAGYAFAKLRFGGR
                     DRMFGLLLTAIIVPPQVGMLPLFLLMKTLHLVNTYWGVIIPSMVTVFGIFLIRQFMLS
                     VPQELLEAARIDGASEWRIYWSVVMPLARPILATLATFMFMSTWNDFMWPLIILSNQS
                     HHTLPVALANLSGEHVLDVELMMAGSVVTILPVLIVFLVLQRYYIAGLMVGSVKG"
     gene            3554..>3899
                     /locus_tag="VNN25_01440"
     CDS             3554..>3899
                     /locus_tag="VNN25_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90214.1"
                     /translation="MRSTSAARADRESRNAVSLRLTYGHEGPQCGHLSAPARACCCKR
                     YTSASRRTAPDRPSQPDLQDESLRWDAVAEEARAFLNGIEGAALIRRQRLALIAGQGY
                     TIGPQLARDPAKA"
BASE COUNT          646 a         1287 c         1205 g          761 t
ORIGIN      
        1 cactgaccgc catacccgat gtggtgtcat cctgagccgc caacggacgg cgaaggatct
       61 caaaatgcga cgcgcatggc tcgtgccgcg taatttgaca ctcgctcttg cgctgttgct
      121 gctcactacc tgttcctcac aaacctccga ccaccgcgag cgcctcgaat tctgggctct
      181 cggcagcgaa ggagaggcag tggccagctt catccccgag ttcgagcggc gcaatccgga
      241 tattcatgtc gtcgtgcaac agatcccgtg gtctgccgcg cacgaaaagc tggtcaccgc
      301 gtttgtcggc gatgccatgc ccgacatctc gcacatgggc aatacgtgga tgccggagtt
      361 cgtcgctctc aatgccctcg atgatctagg gccctatgtt gcgtcgtccg cggtggtcaa
      421 gcagtccgac tatttccccg gaatctgggc aacgaacgtc gtcggcgcga cgctgtacgg
      481 aatcccgtgg tacgtcgaca cgcgcgtgct cttttatcgg agcgatgttc tggcagaggt
      541 cggatttccg catggcccgc gcacgtgggc ggagtggcgc gatgcaatgc agcggattca
      601 cgatcagaag aagtgccgct gggcgattct tctgccgacg aatgagtacg agccgatcac
      661 gatgctcgca ctctccaatc acgctacgct gttgaccccg gacggaacgc gcggcgcatt
      721 ttcggagccg ggctttgccg gcgcgttcgc gttctatgtc gacacgttcc gaagagggtg
      781 ggcgcctgcc gtcagcaaca gcgagatcgc gaatctctac cagcagtttg ccgagggcga
      841 cttcgcgatg tacatcacgg gcccgtggaa cgtcggggag ttccgcaagc ggctgccgcc
      901 ggaaatgcag gataagtggg ccactgcgcc actcccagcc gtcaatgcca gcgagccgat
      961 gggcatttcg atggccggcg gcggcagtct tgtcgtattt cgcgcctcgc ggcacaaagc
     1021 cgccgcgcga aagttgatcg attttctctc cgagcccgcg cagcaggtgc gtttctatga
     1081 actggtcggc gatctcccgg cgcgccgcgc ggcctggcag tcgccgctgc tggccaatga
     1141 caagcatttc cccgcgttcc gtgcgcagct cgagcgcgtg acgccgatcc ccaaggttcc
     1201 ggagtgggag cagattgcca ccgcgatcta cgaccgcggc gaggcggccg cgcgcggagc
     1261 ggtgccgccg gcggtcgcgc tccgtcagct cgacgctcgt gcggacgagt tgctggagaa
     1321 gcggaggtgg atgctggcgc aatcgcggtc gcgaggtgcg gcggccacgc agtctcgcgt
     1381 cggagagtct ccatgaaaac ccccgaagcc cgcgctgcgc ttctctttct ggcaccggcc
     1441 atcgtgctgc tgtcggtgtt cttctttgcg ccggtcatcg cgggattcgc gctcagcgtc
     1501 acggacttcg acctctattc gatcggcgac atccacaacc ttcgtttcat tgcgctgcgc
     1561 aactacggcg acctcctcgg caatccgatc ttctggacgg ccttcgcgaa cacgctctac
     1621 tttgcgctcg tcggcgggcc gctgaccgtg gcgatttcgc tcgtggcggc actgctggtg
     1681 aatgcgaagc tcacgcgctg gaaggcgctc tttcgcacga tctactttgc gccggtcgtc
     1741 acgacgctcg tcgcggtgtc gatcgtcttc aagtacctct accatccgcg cttcggcatc
     1801 atcaatctcg ccctcggcgc catgcacctg ccgcagcccg actggctcgg cgatcctcac
     1861 tgggccatgc ctgccatcat cctgctcggc gtctggaagg gcttcggcta cacgatgatc
     1921 atcttcatcg ccgggcttca gaacgtcccg gaggagctgt acgaagcggc gcgcctcgat
     1981 ggggcaggga tgtggcggca gttccggcac gtcacgttgc cgatgctcgg accgacgttc
     2041 ctcttcgtcg gcgtcgtcgt cgccatcggc caactgcaga tcttcgccga gccgtatgtc
     2101 atgaccaagg gcggcccgct caacaagacg ctgacgatcg tcatgatgat gtacgagcag
     2161 ggcttcaaat tctggcgcat gggatacgcc gcggcggtgg cgttcgtgct ctttctcatc
     2221 atcggcgcag ccacgctggt gcagatgcgg ttgcgggaga agcaatgagc cggcggagcg
     2281 aatcgctgct cctccactcc gtgctcatcg ccggcggctt cctgacgctt tttccgttgc
     2341 tgtggatgct ctccgcgtca ttcatgtcga atggggagtc gaccaccttt ccgccgcact
     2401 tgttgccgca tgccgccacc ctcggtcagt accggcagct ctttctgcgc ctcaacatcg
     2461 gccgcgcctt tctgaatagc gccatcgtgg catcgacggt caccatctcg tcggttctct
     2521 tcggatcgat ggccggctac gccttcgcca aactccgctt cggaggacgc gaccgcatgt
     2581 tcggtcttct cctcaccgcc atcatcgtgc cgccgcaggt ggggatgctg ccgctctttc
     2641 tgcttatgaa gactctgcac ctcgtcaaca cctactgggg cgtgatcatc ccgtcgatgg
     2701 tcacggtctt cggcattttc ctcatccgcc agttcatgct ctccgtgccg caggaactgc
     2761 tggaagcggc ccgcatcgac ggcgccagcg aatggcggat ctactggtcg gtggtcatgc
     2821 cgctggcgcg gccgattctg gccacgctgg caacgttcat gttcatgtcg acgtggaacg
     2881 acttcatgtg gcctctgatc atcctctcca accagtcgca ccacaccctc cccgtcgcac
     2941 tggccaacct cagcggcgag cacgtgctcg acgtcgagtt gatgatggcc ggatcggtcg
     3001 tcaccatcct gccggtgctc atcgtcttcc tcgttctgca gcggtattac atcgccgggt
     3061 tgatggttgg cagcgtgaag gggtagcggt ggccatcggc agaacgttgt catcctcgta
     3121 acggacggcc tcgagaccgt cgaacgcgag accggcaacg cacgtcgcgt gcgtccccgt
     3181 gccgacagcg ctgcaccctc ggcgcgcacg cgcggaacct ggtgtttgca gcaacgttgc
     3241 gcccccctgc acacgttcgg acgcggcccg cagcagcgta gttccatcgg gccgcagtgc
     3301 ggacacggcg tgccgcaacc ccgtcacctt gcgccgcaca tgccgccatc atgagcctca
     3361 atgcggccat ggagagccgc aatgcggtca gctcgcgccg cacgtgcgga cagagagagc
     3421 cgcaatgcgg tcaactagtg ccgcacgtgc ggacaggaag agccgcaatg cggtcaacta
     3481 gcgccgcacg tgcggacagg aagagccgca atgcggtcaa ctagcgccgc acgtgcggac
     3541 aggaagcgcc gcaatgcggt caactagcgc cgcacgtgcg gacagggaga gccgcaatgc
     3601 ggtcagcttg cgcctcacgt acggccacga agggccgcaa tgcggtcacc tcagtgcgcc
     3661 tgcgcgcgca tgttgctgca aacgttacac ttcggccagc cggcgcactg cccctgacag
     3721 gccgtctcaa ccggatttgc aggacgagtc gctccgctgg gatgccgtgg ccgaagaggc
     3781 gcgcgcgttc ctgaacggga tcgagggagc agccctcatt cgccgtcaac ggctagcgct
     3841 catcgcaggg caggggtata cgatcgggcc gcagctcgcc agggatccgg ccaaggcag
//
LOCUS       DATLVW010000040         6306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_208679, whole genome shotgun sequence.
ACCESSION   DATLVW010000040 DATLVW010000000
VERSION     DATLVW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208679"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..723
                     /locus_tag="VNN25_01445"
     CDS             25..723
                     /locus_tag="VNN25_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HXH90215.1"
                     /translation="MGIGVATGADNVFILSSEKADQVESDRLVPLVMRSDIQEGNIEW
                     AGRFVINTFGPDGRAVDLSWYPRLAAYLAGHEHAVRARHVSRRGNAFWFRTIDRVYPD
                     LVERPKLLIPDIAGANEVAFDAGGYHPHHNLYFVTSDIWDLEVLGGLLSSRVALFFIW
                     SYAVKMRGGYLRFQAQYLRRIRVPRPESLSTSLKRQIRVAFKTRDFGKLDRLALLAYG
                     LGGLPEFSFIDTRK"
     gene            complement(1499..2575)
                     /locus_tag="VNN25_01450"
     CDS             complement(1499..2575)
                     /locus_tag="VNN25_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH90216.1"
                     /translation="MAIIRAALVDDADDDLKIAGRLLRTGIQCDPIRPPDEENSFEQL
                     INQLRAGNYDIVLLDYRLDDEVPRGYRGGTVAARLKEFHPDMPVVLLTTEEKLEASVR
                     HNPRVAALFDHLVLKEDLAHADTRKRRAAEMHDLVASYQILKEEEAQPNDDLYDIVSR
                     LARATEDDRFAPSVRNRAARTPELANWFLQEFLAYPGSVLPISDVAARLGIAQTAAEG
                     DAVRSWLAPASYAGVFATIRERWWRGRLDLRLIGKRSDLRDKPATARAAFISKAIGVR
                     IPPARCAWCGGEYVERACAICKEAVDASHFLVGNIDDRPGWAEPARVCFRCIAQGDAE
                     DVRFITGAEQIAHKLRTGELKRPA"
     gene            complement(2594..4717)
                     /locus_tag="VNN25_01455"
     CDS             complement(2594..4717)
                     /locus_tag="VNN25_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXH90217.1"
                     /translation="MSQTGTLQFAIDARHIRQLGRELVADKITAVAELIKNAYDADAT
                     TVTLRFGGDTERSGGCLQITDDASGMTFVDVRDRWMRLSTDYKARTPRSSRFQRWRAG
                     QKGIGRFATETLGEKLVLTTTSETTGEHLQITFDWTEHYSAGTELQSVENKYVLSVGN
                     PEQPFTTLHIERLHDSWNKGDMERIGRAVYLLQPPRPPEKVGDDPGLVVHIISADGTE
                     VRIEPDLDAFLDAATAVITGAVDENGIGRWQIRSDRFELDEERILPDRILTTGAFRFR
                     AYYFIYARAALGNVAVGRARAFGRDYGGIRLYRDDLRIMPYGEPTDDWLRLADIYRRR
                     EYLPPIGNNAFFGEVAITRDENVLMIDTASREGVVENESLRALRVFVSDGLLWGILQV
                     AAARGKKGKSTRRKKDAPTRVDLLVRALAAVGAPANEETAARLSAVVDEIMADAARAD
                     AEQQERVQDLIGELELLRVLASLGTSIAVFSHELKAIMNASSGALLDVEDALTGGVDI
                     EMVRASVNNLGEAMGKLDHLGEYIATYVSDSRRRERVPQPLHAIIKEFVSSFERTVNK
                     RGITFDPDVVEPETLRTTPMSRSQIQAILFNLLTNAIKAMDGEGPQTRRIRISARRDG
                     NYASLRFEDTGAGVPAELRHRIFDPFVSASLPSEQELGVGTGLGLKIVRDIVTAYNGD
                     IDLVEATEGFTTAFEVRLPIWTDSV"
     gene            4899..5495
                     /locus_tag="VNN25_01460"
     CDS             4899..5495
                     /locus_tag="VNN25_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90218.1"
                     /translation="MSSRLSAIGFLIFVSMTLSALAADSAGKWEASRSGSGRLLTYSG
                     VATVLGKATPVTVKFFCDTTYTANEKGALGFDLTLGHVAALAPFDFDDFEGPDAPAVA
                     KELLVLTITRKGQAPLSFKVAPSGSYPEEGSFTFEGSDVTHKAVSTAKSILRALGDDA
                     DELRFTITDYRNAKVKIELTIPVAGKQADFKALLAGVK"
     gene            complement(5580..5996)
                     /locus_tag="VNN25_01465"
     CDS             complement(5580..5996)
                     /locus_tag="VNN25_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH90219.1"
                     /translation="MAQLNGYLFFNGNCAEAMGFYAKTLGGKVDIMKVGKSPAAEQMP
                     PGSDDLVIHARLELPGGAVLMASDWLDKTPYPSMKGFALSVGYPTGDEAHRTFDLLVE
                     GGNAPMPFGKTFFAEGFGMLTDRFGTPWMVAGGMMG"
     gene            complement(6042..>6306)
                     /locus_tag="VNN25_01470"
     CDS             complement(6042..>6306)
                     /locus_tag="VNN25_01470"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HXH90220.1"
                     /translation="AHNYNAIAIVKLETHDITTELHGTVKSISASQWVIGGPPGSLAP
                     DFIVKIASTTVIYPDPKVGDRVVVVGTRDATGTFTATKISKEP"
BASE COUNT         1200 a         1819 c         1885 g         1402 t
ORIGIN      
        1 ctcgaagatg atggcgctac acgggtgggc atcggcgtcg ccacgggtgc ggacaacgtg
       61 ttcatccttt ctagcgagaa ggcggatcag gttgaatctg accggcttgt accccttgtc
      121 atgcgttccg acattcaaga agggaacatc gagtgggcgg gccgctttgt catcaacacc
      181 ttcggtcccg acgggcgtgc cgtggacctt tcttggtatc caagacttgc ggcttacctg
      241 gccggtcatg agcatgctgt acgcgcccgg catgtttccc ggaggggcaa cgccttctgg
      301 ttccgaacga tcgaccgtgt ctatcccgac ttggtggaac ggccgaagct actcatcccc
      361 gacatcgcgg gcgcgaacga ggttgccttc gatgctggcg ggtaccatcc gcatcacaac
      421 ctgtacttcg tcacttccga tatctgggat ctcgaagttc tgggtggcct tcttagctcg
      481 agagtggctc tcttcttcat atggtcctat gctgtgaaga tgagaggtgg atatctgcgc
      541 tttcaagcgc agtaccttcg ccggatacgc gttccgaggc ctgagtcgct ctcaacgtca
      601 ctcaagagac agattcgggt agcattcaaa acgcgcgact tcgggaagct tgacaggtta
      661 gcgctgcttg cgtacggact cggcggactg cccgagtttt cgttcattga tacgcgtaag
      721 taagcgttag acccggtgtg tccgccactg gatgctcatt tggccggtcg cggattccaa
      781 tcgctcgcat tattcgcttg cgctgcaagc caagagttcg ccgtggaggt tgtgttcatg
      841 taggtgtcca gccgcctcat gtagtgcgtt aggacgagcc tttcggcggt ttttacatgt
      901 tcccgccatg gattcgttcc gctgactctc ttccaggcct ccgggtaaaa cgaaaacagt
      961 acgaattcga cgatcagtat gaagtcggta ggacttgacg gccatcgagg aaccttaagc
     1021 cacatcgtgt ccggtttctt gccttgcgca ggttttccgc ctagctgaag gtgccaggcg
     1081 ggtcctgctt gcccggggtt ggcgaggtcc agatggtgcc ggacgatgag atcatcggtt
     1141 cccgcttcgt agacctcgat ggctacggtg cagttcgcca acggcgctgc gcaccaatcg
     1201 cggggtttcg atagagtctt tgaggagaag tcgaggtcac actcgaggcg caggtcgacc
     1261 gtgtgtgtat cgatcgcgac gggcacaaag cgtagcgtgg tcgcgggtct accaaacctg
     1321 attctccagc ctgtgcattt gcgcggattt gggctattgc tcctacaggc atggcatcgc
     1381 gtcacgacgc gactcggagt gtcggcgacc acccacgagg cgatgacccc ctggcgcacc
     1441 agttcttggg cgacgaagac cacaacttcc tcagcggcgg ggcagcacca gggttccttc
     1501 atgctggccg tttgagttca cccgtgcgga gcttgtgggc gatctgttcg gcacctgtga
     1561 tgaatcgaac gtcctcagcg tcaccctgtg cgatacagcg gaagcatacc ctcgctggtt
     1621 ccgcccaacc aggtcggtca tcaatattgc caactagaaa gtggcttgcg tcgactgctt
     1681 ctttgcagat cgcgcacgcg cgttcaacgt actcgcctcc gcaccatgca catcgcgccg
     1741 gtgggatgcg cacccctatc gccttggaaa tgaaggcggc gcgcgccgtc gccggcttat
     1801 cacgcaagtc cgacctcttt ccaatcaaac gcagatcaag tcgcccgcgc caccaccgtt
     1861 cgcggatagt tgcaaatact ccagcatagg atgctggagc caaccaggac cgcaccgcat
     1921 caccctccgc tgctgtttgt gcgataccaa ggcgcgcggc tacgtccgat atcgggagga
     1981 ccgacccggg gtaggcaagg aactcttgca aaaaccagtt tgcgagttcc ggtgtgcgtg
     2041 ccgccctatt gcggactgaa ggtgcgaatc gatcgtcttc ggtcgctctt gccaggcgac
     2101 tcacaatatc gtagaggtca tcgttcggct gagcttcctc ttctttcaag atctgatagc
     2161 tcgcgacgag gtcatgcatt tcagccgctc gacgtttgcg tgtgtctgca tgagcaagat
     2221 cctccttcag aacaaggtga tcgaagagcg cggctacccg cgggttgtga cggaccgacg
     2281 cctcgagctt ctcttcagta gtcaggagca cgacaggcat gtccgggtga aattccttca
     2341 gccgtgccgc gaccgttccg ccgcggtatc cacgcgggac ttcgtcatca aggcggtagt
     2401 ccaagagaac aatgtcgtag tttcccgcac gtaattggtt gatgagctgt tcgaaggagt
     2461 tctcttcgtc cggcggacga attggatcgc actgaatgcc agttcttagt aaacgtccag
     2521 ctatcttgag atcgtcatcg gcgtcatcaa ccagcgccgc ccttattatt gccatcgagt
     2581 cccctttctt tgtctacacc gagtcagtcc agatagggag ccgaacctcg aatgcggtcg
     2641 tgaagccctc ggtcgcctcc accagatcga tgtcgccgtt ataagccgtt acaatgtccc
     2701 tcacgatctt taggcccagc cctgtgccca caccgagttc ctgttcggat ggcagactgg
     2761 cactgacaaa tgggtcgaaa attcggtgtc gaagctccgc gggtacacca gctccggtgt
     2821 cttcgaaacg gaggctcgcg tagttaccat cacgacgcgc gctgatacgt atccgccgag
     2881 tctgaggtcc ctctccgtcc atagctttga tcgcatttgt caacagattg aagagtatcg
     2941 cttggatctg agaacgactc attggcgtcg ttctcagtgt ttcgggctcg acgacgtcgg
     3001 ggtcaaacgt gatcccgcgc ttgttcactg tccgctcaaa ggagctgaca aattccttga
     3061 taatggcatg cagtggttga ggcactcgtt cgcgccggcg actatcgctg acgtatgtgg
     3121 cgatgtactc gccgagatgg tctagcttac ccatagcctc gcccaagtta ttgacactcg
     3181 cgcgtaccat ctcaatatca actccacccg tgagtgcgtc ttcgacgtcc aggagggctc
     3241 cgcttgaagc attcatgatt gccttgagtt cgtggctgaa tactgcgatc gaggtcccga
     3301 gtgatgccag cacgcgcaac aattcgagct cgccgataag atcctgcacg cgttcctgct
     3361 gttccgcatc ggcgcgcgcc gcatcggcca taatctcatc gacaacggca cttagccgcg
     3421 ccgcggtctc ctcgttcgcc ggcgcaccca cagctgcgag cgcccggacg aggagatcta
     3481 ctcgggtagg tgcatctttt tttcggcgtg tcgacttgcc cttttttcca cgcgccgcgg
     3541 caacttgcag tatcccccag agtagtccgt cgctaacgaa gacgcggagg gccctcaagg
     3601 attcgttctc gacgacgcct tcgcggctgg cggtgtcgat catcaggacg ttttcgtctc
     3661 tcgttatagc cacttcgccg aagaaggcgt tgttgccgat aggcggcagg tactcccgtc
     3721 ggcggtagat gtcggcgagc cgaagccaat cgtcagtggg ttcaccgtac ggcatgatgc
     3781 gcagatcatc cctgtacagg cggatgccac cgtaatcgcg accgaaggcc cgcgcgcgcc
     3841 ccacagcgac attgcccagc gcggcgcgcg cgtagatgaa atagtatgcg cggaatcgaa
     3901 acgcgccggt ggttagtatt cgatccggaa ggattctttc ttcatccagt tcaaagcgat
     3961 cgctccgtat ctgccagcgg ccaattccat tctcgtcgac tgcgccagtg atgacagctg
     4021 tagcagcgtc gagaaaggca tcaagatccg gctcaatgcg aacttcggtc ccatcggcgg
     4081 agataatgtg aacaaccagt ccggggtcgt caccaacttt ctcgggtggc cgaggcggct
     4141 gcaacaggta aacggctcgg ccaatgcgtt ccatatcgcc cttgttccac gagtcgtgca
     4201 gccgctcaat gtgcaaggtc gtaaagggtt gttccgggtt gccgacactg aggacatatt
     4261 tattctcaac cgactgcagc tctgtcccgg cgctgtagtg ctccgtccag tcgaatgtga
     4321 tctgaaggtg ttcaccagtg gtttcgcttg tagttgtgag aacgagcttt tcaccgagcg
     4381 tttcggtggc aaagcgacct ataccctttt gccccgcgcg ccaacgctga aaacggctgg
     4441 agcgaggagt gcgcgccttg taatcggtag aaagacgcat ccatcgatcg cgcacatcga
     4501 cgaaagtcat cccactggcg tcgtcggtga tctgaaggca accaccggat cgctccgtat
     4561 cgcccccgaa gcgaagcgtg accgtagttg cgtcggcgtc gtaggcgttt ttgatgagct
     4621 cagccactgc cgtgatcttg tcagcgacaa gctctcgccc gagctggcgg atgtgacgcg
     4681 cgtcgatcgc gaactgcagc gtcccggttt gcgacatcgt ggaggaggtt tctaccacac
     4741 tcggcctcac cgtcgagtac tgtcgggaga atgtccgatt gctacgaacg ggtggacgcg
     4801 acaacctcgc cttcatcgca gtgagtggtg ctgtgctacc gtcccgcgcg ctggatcagc
     4861 aggacactgg atgcgccacc actgatggag aacttgtaat gtcgtcacgc ctctctgcaa
     4921 ttgggttcct cattttcgtt tcgatgacgc tctcggcgct ggccgcggat tcagccggga
     4981 agtgggaggc gtcgcggtcg gggagtgggc ggcttctgac gtactccgga gtcgctaccg
     5041 tgctggggaa ggcgacgccg gttacggtga agtttttctg cgacacgacg tacacggcga
     5101 acgagaaggg cgcgcttgga tttgatctga ctctcgggca tgttgcggcg ctggcaccgt
     5161 tcgacttcga tgacttcgaa ggaccggatg cgcctgcggt ggcgaaggag cttctcgttc
     5221 tcacgattac gcggaagggg caggcgccgc tgtcgttcaa agtggcgccg agcgggtcgt
     5281 atccggagga gggaagcttc acatttgagg gatcggacgt gacgcataaa gcagtcagca
     5341 cggcgaagtc gatcctccgc gcgcttgggg acgatgcgga tgagttgcga ttcacgatca
     5401 ccgactatcg caacgcgaaa gtgaagatcg agctgacgat tccggtggcg gggaagcagg
     5461 cggatttcaa agcgctgctt gctggagtga agtgacgggg agtggggagt tgggggtggg
     5521 gagtgggaga acgacgtctt cgtagacatc attcatcaca ccctctcccc aaacaatcct
     5581 cagcccatca ttccgccggc gaccatccac ggcgtgccga agcggtctgt cagcatgccg
     5641 aagccctcgg cgaagaacgt cttgccgaag ggcatcgggg cgttgccacc ttcgaccagc
     5701 agatcaaagg tgcgatgcgc ttcgtccccg gtcggatagc cgactgagag cgcgaagccc
     5761 ttcatcgatg gatagggcgt cttgtccagc caatccgagg ccatgagcac ggcgccgccg
     5821 gggagctcga ggcgggcgtg gatgacgaga tcgtcggaac ccggtggcat ttgctccgcg
     5881 gccggcgatt tgccaacctt catgatgtcg accttgccgc cgagcgtctt cgcgtagaaa
     5941 cccatcgctt ccgcgcaatt accgttgaag aacaggtagc cgttgagttg ggccatatcg
     6001 aattgcttct ccttgttcga ttcagcttcg ttgcggctga atcagggttc cttgctgatc
     6061 ttggtcgccg tgaacgtccc ggtggcgtcg cgggttccga ctacgaccac acggtcgccg
     6121 actttgggat cgggatagat gaccgtcgtc gaggcgatct tgacgatgaa gtcgggagcg
     6181 agcgagccgg gagggccgcc gatcacccac tgcgaggcgc tgatcgattt cacggtgccg
     6241 tgcagttcag tcgtgatgtc gtgcgtctcg agcttcacga tggcgatggc gttgtagttg
     6301 tgcgcg
//
LOCUS       DATLVW010000041         8177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_333728, whole genome shotgun sequence.
ACCESSION   DATLVW010000041 DATLVW010000000
VERSION     DATLVW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333728"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1132
                     /locus_tag="VNN25_01475"
     CDS             215..1132
                     /locus_tag="VNN25_01475"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:398..400,aa:Sec)
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HXH90221.1"
                     /translation="MFHPLAGAAYVHADAASFVDNADLVIAVERNGEAAAYPIRQLAY
                     HHLVQDVVGGVPIVVTYUTLCHTGLVWETTVDGRTLHFHLAGINNQNFIMRDEETGSW
                     WQQISGEAIQGPLKGTRLKHVPQDEIGFALWKREHAAGRVLRPDAKIAAAGLYAGADW
                     EAHIAKMPVATNTHDARLQPRELIVGVTVDGRSKAYTMSDVKQQNPIIDTIGAVPIVI
                     AVAEDGRSVRAFRRVVDGRVLELFAKPSSSPITFVDSATGSEWDFGGRAITGPLAGHQ
                     LTKIDVLSDYWFDWRTYHPDTAIFRVGLK"
     gene            complement(1226..3019)
                     /locus_tag="VNN25_01480"
     CDS             complement(1226..3019)
                     /locus_tag="VNN25_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXH90222.1"
                     /translation="MVAFSSAITAAVAASPPKHTNRLARETSRYLLQHATNPVDWYPW
                     GEAALKAAREQNKLIFLSIGYSTCHWCHVMEAESFSNDAIGAAMNEHFIAIKVDREER
                     PDIDSAYMAIARKLMSDPGWPLNVILTPEGKPFFAASYIPRDRLQTLIERLGTSWKEH
                     PEQIASTAAMVMQSLAPEAAENERAPDAAMLKTAYQQLAERFDAVNGGFLPPPKVPAA
                     HQLMFLLRYWRRSGDKHALEMVETTLRAMRRGAIWDKARSGFHRYTVDAEWREPHYEK
                     MLCDQALLAIAYLEAYQATHKLEYASTAREIFTYVLRDLRAPSGAFYSASDSDERGVR
                     DEKILTDWNGLMIAALASGAVVLDDETYAIAAKRAAAVVLADERLQHQRSHPAYLDDY
                     AFAVWGLLNLYEATFEVQYLDRAISLERESLSRFRDSDGRFYMTANDSETLLVRPREA
                     SDGSLPSGNSVQLMNLVRIARITGDPFFDKAANDLLRTSAGEVDVIPSASAHLMSAVD
                     FLIGPSFEIVLAGTDVHPLQRAVYASFVPNKVVLRSGADVARIAPFTKMQKAIRGKAT
                     AYVCTNQQCKLPTNDTGKVAELLGATPARPR"
     gene            complement(3092..6190)
                     /locus_tag="VNN25_01485"
     CDS             complement(3092..6190)
                     /locus_tag="VNN25_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXH90223.1"
                     /translation="MAQLSILAICFSASALLAQVPTIINEIDQLESVRDPKCYATASR
                     LEDFIYGTPLDFDARAEKIALQKQFIRDLWLKASAAAASAGKTQIDVDTLRPILQAAV
                     PYVATAGGDWVVRPDDAHRTEVSARDRRQYGSVAYALRAILAVQQDAFVDGTKIVPLD
                     PPAVELFKESIDLVTLAALQHADHATRATNHERLGAKELKAGWADVIGHAPAVAPEAK
                     PAHVASATAPQKFATINAIVAEKLAAYEKYNDLSQAVFLRNVQVYMSRHMWPTDPAEG
                     KAFKDQFTETMIAWTGDVMLEAEKRAKKRGHALIRVEDVHDAVQLFEPHVLNEYEDCI
                     YFPRLPKNEQVVIEAYDLDAFRDPGLHWFYLKEVLADPKYKGTLEPDPFAAELLTEGG
                     AQMGTLILRVAGKIATAEGKDRLDRTHLSRGVTEIQRLIDKNASMPPEKKTAVTIASA
                     STPPAKTGGKYFTDVTASSGIQFEHRMSDWLARLIRSYTVKEGDKLVLAVPPAFGGSG
                     IAAEDIDNDGDIDVLLLSGSGNALYLNDGKGHFTDVTESAGLNWKRPDGRPGEPRQAI
                     IADFDNDGLEDIFITYVDDDHRLYKNLGHAKFEDVTARAGLGGKGLVGGPATAFDYDK
                     DGLLDLYIGYFGDYIHGELPTFARRNTNALPDKLFHNKGNFVFEDVSAGSGVENPGWA
                     QAVGHLDFDGDGWEDLICGNDFGSNSWYRNLGGTGKFEDVSAKLGTDKPSYTMNIGIA
                     DLNRDGYPDVYISNIVTMNKDEKYVLPDAKTRLKLNPQKMANMRVVEANDLWESAAAN
                     GKLNSYVQSTAIGRGYKSTGWSWGADFFDFDNDGDDDLYLVNGMNEYAVYSSVNPYFT
                     DATGAQQQAVVPVAEKEAPVFFVNRNGMLIEDSAQSGADPLGNARAVAQFDFDGDGDL
                     DMMVNNFNGPAILYRNNSETNGNHWIKIRLTGDPSKGVTRDAIGAKIIVDTAHQKGMW
                     REVFSTIGYLTSNPKEQHVGLGTDKHADVTVIWPSGEKQTFKGLAADKSYRIVQGTGL
                     VK"
     gene            complement(6403..6549)
                     /locus_tag="VNN25_01490"
     CDS             complement(6403..6549)
                     /locus_tag="VNN25_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90224.1"
                     /translation="MAELREWILEREAEVWDREIERDAASGKLDKLFEKSVADHLAGK
                     SREI"
     gene            complement(6561..7787)
                     /locus_tag="VNN25_01495"
     CDS             complement(6561..7787)
                     /locus_tag="VNN25_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90225.1"
                     /translation="MNVTFTGTPPFQGVWSDGEHFFSNETSLVRHVQIPGDYRIATFK
                     DAVCDGTQTGVAVVPPLIPTAQVWASGNGCALVDSAYVNFSGKAPFSACWNDGTCFTT
                     FSSQIVRPLTTVTPMLTLASGSDRDGCPLAITGGTRVLPSPKAKIERKCLTVGAFEGN
                     NVTEFLVTIDGTFVEQAFPTWSDGSHALFRYFTIDQTTTYTLNSLSVYGWQCAPLIDP
                     PRSVTVYPTPVPALSSSAADVCAGSTFSAAVTPPPPGTQVTWEMFNGTILSGQGTNAV
                     QVQGVAGPDGNITGYLYCTFTFSDPTHCPLASRTQVRVVTDPYATLSLDSTPLHSGKT
                     NYISFTLGYTVTGWSLENSMGDPITLVGKCDITNGGTCTATYTSTHGPGQSTITLHAK
                     SACPTKDVSGVVTIVP"
     gene            7786..>8177
                     /locus_tag="VNN25_01500"
     CDS             7786..>8177
                     /locus_tag="VNN25_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90226.1"
                     /translation="MSAEPHERGPELTMAFGLSQGGFGTMAKLRSAALMQAPRVKRTA
                     RPTLPLDPAVTVTESPVAVAGVPFVIVHEATAPGCGRTDAEKTVSGMTESGPSMAAGG
                     LEQTPIVYGIAAWAVEQAEALTGLFATAR"
BASE COUNT         1496 a         2477 c         2558 g         1646 t
ORIGIN      
        1 gccattccgt ccgcagtcgg cgcgcggagt ctcgatctcg tatgcgctgc ggacgatttc
       61 gccgatcgtc acgattgttg cgctgctgat cgtcgcgtgg ctgatcttca cgctctggcg
      121 aggcgcgcga tggtggggga gaatcggcat cgtcattctt cttctgttaa cgaccgtcag
      181 cacttggttc gcgcggcaga atcattttga gtggatgttt catccgctcg cgggcgccgc
      241 gtatgtgcac gcggacgcgg cttcgtttgt cgacaacgcc gatctcgtga tcgccgttga
      301 acggaacggc gaagcggctg cctatccgat ccggcagctc gcgtatcacc atctcgttca
      361 ggacgtcgtc ggtggcgtac cgattgtcgt cacctattga acgctctgcc acaccggtct
      421 ggtgtgggag actacggtcg acgggcgaac gctgcacttc cacctcgccg ggatcaacaa
      481 ccagaacttc atcatgcgcg acgaggagac cggctcgtgg tggcagcaga tcagcggcga
      541 ggcgattcag ggaccgttga agggaacgcg gttgaagcac gttccgcagg atgagattgg
      601 attcgcgctc tggaaacggg aacacgccgc cggccgcgtg ctgcgccccg acgcgaagat
      661 cgccgcggcc ggactctacg caggcgctga ttgggaggcg cacatcgcca agatgccggt
      721 ggcgacgaac acgcacgacg cacgcctgca accacgcgag ctcatcgtcg gcgtgacggt
      781 cgatggccgg tccaaggcgt acacgatgtc tgacgtcaaa cagcagaatc cgattatcga
      841 cacgatcggc gcggttccga tcgtgatcgc ggtagccgag gatgggcggt cggtgcgcgc
      901 gttccggagg gttgttgatg gacgtgttct cgagctcttc gcgaaacctt catcgtcacc
      961 gatcacgttc gtcgatagcg caaccggaag cgaatgggat ttcggcggcc gcgcgatcac
     1021 cggtccgtta gcaggccatc agctaacgaa gatcgatgtt ctcagcgact actggttcga
     1081 ctggcggacg tatcacccgg atactgcgat ctttcgggtt gggttgaagt gagtttcaaa
     1141 gacagccttt cacgcggaga cgcggaggtg cggagggaga tgagttgctg ttctccgcat
     1201 ctccgcgcct ccgcgtgact tcgagctagc gaggcctagc cggagtcgct ccaagcaact
     1261 cggccacctt ccccgtgtcg ttcgtcggca gcttgcactg ctgatttgta caaacgtacg
     1321 ccgtcgcctt tccacggatg gccttctgca ttttcgtgaa cggtgcgatt cgcgcgacat
     1381 cggctcccga tcggaggacc actttgttcg ggacgaacga tgcgtagacg gcgcgttgca
     1441 gcggatgaac gtcagtgccc gcaagcacga tctcgaatga tgggccgatg aggaaatcga
     1501 cggcgctcat gagatgggcg gatgcggagg ggatgacgtc gacttcgccc gcgctcgtgc
     1561 gcaggagatc gttcgccgcc ttgtcgaaga acggatctcc tgtgatgcgg gcgatgcgca
     1621 ccaggttcat gagctgcacc gagttgccgg acgggagcga cccgtcgctc gcttcgcgtg
     1681 ggcggacgag cagcgtctcg gagtcattcg cggtcatgta gaagcggccg tcagaatcgc
     1741 gaaaacgaga caacgactcg cgctccagcg agatagcgcg atcgaggtac tgcacctcga
     1801 acgtcgcctc gtacaggttg agcaggcccc agacagcgaa ggcgtaatcg tcgagatacg
     1861 cgggatggct gcgctggtgc tgcaagcgct cgtccgccag cacgacggcg gcagcgcgtt
     1921 tcgccgcgat tgcgtatgtc tcgtcgtcga ggaccacagc gcctgacgca agagctgcga
     1981 tcatcaggcc gttccagtcg gtgaggatct tctcgtcgcg gacgccgcgc tcgtcggaat
     2041 cgctcgcgct gtagaacgcg ccgctcggtg cgcgcagatc gcgcagcacg taggtgaaga
     2101 tctcgcgcgc tgtgctcgcg tactcgagct tgtgcgtggc ctgatacgcc tccagatacg
     2161 caatggccag cagcgcctga tcgcagagca tcttctcgta gtgcggctcg cgccactccg
     2221 cgtcgacggt gtagcgatga aagcccgatc gtgccttgtc ccagatcgcg ccgcggcgca
     2281 tcgcccgcaa cgtcgtctcc accatctcca gcgcatgctt gtcgccgctg cgccgccagt
     2341 agcggagcag aaacatgagc tgatgcgcgg ccggaacttt cggcggagga agaaagcctc
     2401 cgttgacggc gtcgaaccgc tcggcgagct gctggtacgc ggtcttcagc atcgcggcat
     2461 cgggtgcgcg ttcattctcc gcggcttccg gcgccagcga ctgcatgacc atcgccgcgg
     2521 tcgatgcgat ctgctccgga tgttctttcc acgacgtccc caatctctcg atcagcgtct
     2581 gcagtcgatc ccgcggaatg tacgacgcgg caaagaacgg ctttccttcc ggcgtcagaa
     2641 tcacattcag cggccagccc ggatcggaca tcagcttgcg cgcgatggcc atgtaggcgc
     2701 tgtcgatatc cggccgttcc tcgcgatcga ccttgatggc gataaagtgc tcgttcatcg
     2761 ccgcgccgat cgcatcgttc gagaacgact cggcctccat cacgtgacac caatggcaag
     2821 tcgaatagcc gatcgaaagg aagatcagtt tgttctgttc gcgcgcggcc ttcagcgcgg
     2881 cttcgcccca cggataccaa tcgaccggat tggtagcgtg ctgcaggaga tagcgcgagg
     2941 tttcgcgagc gaggcggttc gtatgttttg gcggcgacgc tgcgactgcc gcggtgatcg
     3001 ctgaagaaaa ggcaaccaca gagacacaga gaacacggag gattctctgt gctctctgtg
     3061 tctctgtggt gatcgactct ttcaaattaa ctcacttcac cagacccgta ccctgcacga
     3121 tgcgatagct cttgtccgcg gccagccctt tgaacgtctg cttctccccg cttggccaga
     3181 tcacggtcac gtcggcgtgc ttgtcggtgc cgaggcctac gtgctgctcc ttcggattcg
     3241 acgtcaggta gccgatcgtg ctgaagactt cgcgccacat gcccttctgg tgtgccgtgt
     3301 cgacgatgat cttcgcgccg atggcatcgc gggtcacgcc tttcgacgga tcgccggtga
     3361 ggcggatctt gatccagtga ttgccgttgg tttcggagtt gttgcggtag aggatcgccg
     3421 gcccgttgaa gttgttgacc atcatgtcga gatcgccgtc gccgtcgaag tcgaactgcg
     3481 cgacggcgcg tgcgttgccg aggggatcgg cgccgctctg cgccgagtct tcgatcaaca
     3541 tcccgttgcg attgacgaag aacaccggcg cttccttctc agcgaccggc acgaccgcct
     3601 gctgttgcgc ccccgtggca tcggtgaaat atgggttgac gctggagtag accgcgtact
     3661 cgttcatacc gttgacgaga tagagatcgt catcgccgtc gttgtcgaaa tcgaaaaagt
     3721 cggcacccca cgaccagccg gtcgacttgt acccgcggcc gatcgccgtc gattgcacgt
     3781 acgaattgag cttcccgttc gcggccgccg actcccagag atcgttggcc tcgaccacgc
     3841 gcatattggc catcttctga ggattcagtt tcaggcgcgt cttcgcgtcg gggaggacgt
     3901 acttctcgtc cttgttcatc gtcacgatgt tcgagatgta gacgtcggga tagccgtcgc
     3961 ggttcaaatc ggcgatgccg atgttcatcg tgtagctcgg cttgtccgtg ccgagcttcg
     4021 ccgacacatc ctcgaacttg cccgttcctc ccagattgcg ataccaggaa ttgctgccga
     4081 agtcattgcc gcagatgagg tcttcccagc cgtcgccgtc gaaatcgaga tggccgacag
     4141 cctgcgccca accgggattc tccacgccac tgccggcgct gacatcctcg aagacgaagt
     4201 tgcccttgtt gtgaaagagc ttgtcgggaa gcgcgttcgt gttgcggcgc gcgaacgtcg
     4261 gcaactcgcc gtgaatgtaa tcgccgaagt aacctatgta gagatcgagt aggccgtctt
     4321 tgtcgtagtc gaacgccgtc gccggaccgc cgacgagacc tttaccgcca agacccgcac
     4381 gcgccgtgac gtcctcgaac ttggcgtgcc cgaggttctt gtagaggcgg tgatcgtcat
     4441 cgacgtacgt gatgaagatg tcctccagac cgtcgttgtc gaagtcggcg atgatcgcct
     4501 ggcgcggttc gcccggccgc ccgtcggggc gcttccaatt cagacctgcc gactcggtga
     4561 catcggtgaa gtggcctttg ccgtcgttga gatagagcgc gttgcccgat ccgctcagca
     4621 ggagcacgtc gatgtcgcca tcattatcaa tatcctcggc cgcgatgccc gagccgccga
     4681 acgcaggcgg caccgccagc acgagcttgt cgccttcctt gaccgtgtag ctgcggatga
     4741 gacgcgccag ccagtccgac atgcgatgct cgaactgaat gccgctcgat gcggtgacat
     4801 ccgtgaagta cttgccgccg gttttcgccg gaggtgttga tgccgaggca atcgtgaccg
     4861 cggtcttctt ctccggcggc atcgatgcgt tcttgtcgat cagccgctga atctccgtga
     4921 cccctcggga gaggtgcgtc cgatcgagcc ggtctttccc ttccgccgtc gcgatcttcc
     4981 cggccacgcg caggatgagc gttcccatct gcgctcctcc ctcggtcagc aactccgccg
     5041 cgaagggatc gggctccagc gttcctttgt acttcggatc ggccagaact tctttgaggt
     5101 agaaccagtg caggccggga tcgcggaagg cgtcgaggtc gtatgcctcg atgacgactt
     5161 gttcgttctt cggcagccga gggaagtaga tgcagtcttc gtattcgttg agcacgtgcg
     5221 gctcgaacaa ctgcacggcg tcgtgaacgt cctcgacgcg gatcaaagcg tggccgcgct
     5281 tcttcgcacg cttctccgct tcgagcataa cgtcgcccgt ccaggcgatc atcgtctccg
     5341 tgaattgatc cttgaacgcc ttcccctcgg caggatcggt cggccacata tgccgcgaca
     5401 tgtagacctg cacgttgcga aggaaaaccg cctgcgagag gtcgttgtac ttctcatacg
     5461 ccgccagttt ctcggcgacg atggcattga tggtggcgaa cttctgcggc gccgtcgcac
     5521 tggccacgtg cgccggtttc gcctcgggtg ccacagccgg agcgtgaccg atgacatccg
     5581 cccatcctgc tttcaactct ttcgcgccga ggcgttcgtg gttcgtggcg cgtgtggcgt
     5641 gatcggcgtg ctgcagcgca gcgagcgtga cgagatcgat tgattccttg aacagctcga
     5701 cagcaggtgg atcgagcgga acgatcttcg tgccgtcaac gaatgcgtcc tgctgcacgg
     5761 ccagaatcgc gcgcagcgcg tacgcaaccg atccgtattg ccgccggtcg cgcgcggaga
     5821 cctcggtgcg atgcgcatca tcaggacgca caacccaatc gccgcccgcg gtagccacgt
     5881 acggcaccgc agcctgcagg atgggacgaa gcgtatcgac gtcgatctgc gtcttccccg
     5941 cgctcgccgc cgcagcgctc gccttcagcc acagatcgcg aatgaactgc ttctgcagcg
     6001 cgatcttctc tgcacgcgca tcgaagtcga gcggcgtccc atagatgaag tcctcgagcc
     6061 tgctggccgt cgcatagcac ttcggatcgc gcaccgactc gagctgatcg atctcgttga
     6121 tgatcgtcgg aacctgggca agaagggcgg acgccgaaaa gcagatcgca aggatagaga
     6181 gttgagccat tcgcatagga cgctccgcaa acgtgtctgt ttcggaaagt gtactcggat
     6241 gagaagcgtc ctcagcgcac tcgacgattt cgcacagaag gcgctacgac gaaagaagtc
     6301 gtgcgtattc gtcatgcgcg ctcgtagttc tcacgggcga gacgttggat ttcttcagga
     6361 agctggctga atcgatccca gaaatcgggc tggtgaagtg cttcaaatct cccgactctt
     6421 gccagccagg tgatcagcga cggatttttc gaacagcttg tcgagcttcc ccgaagccgc
     6481 atcgcgttcg atctcacggt cccacacctc ggcttcccgc tcgaggatcc actcgcgcag
     6541 ttccgccaac tcagccgacg ttacggcacg atcgtcacca ccccggaaac atccttcgtg
     6601 ggacaggcgc tcttagcatg gagggtgatc gtcgattgcc cgggaccgtg cgtgctggtg
     6661 taggttgccg tgcacgtacc cccattggtg atgtcacatt tgcctaccag ggtgatcgga
     6721 tcacccatgg agttctccag agaccacccc gtgacggtgt acccgagggt gaagctgatg
     6781 tagttcgtct tgccggaatg gagaggcgta ctatccagcg agagcgttgc ataaggatcg
     6841 gtgaccacgc gcacctgggt ccgactggcc aatgggcagt gggtgggatc ggaaaacgtg
     6901 aacgtgcaat agagatatcc cgtgatgttg ccatcgggac ctgcgacgcc ctgtacttgt
     6961 acggcgttcg tcccctgccc cgaaagaatc gtgccgttaa acatttccca ggtcacctga
     7021 gtgcccgggg gcgggggcgt aaccgcggca ctgaacgtcg aacccgcgca tacatcggct
     7081 gcactcgacg agagtgcggg gaccggagtt ggatagacgg tcacggatcg aggaggatcg
     7141 atcaagggag cgcattgcca cccgtagaca ctgaggctat tgagcgtata ggtcgtcgtc
     7201 tggtcgatcg tgaaataacg aaaaagcgca tgcgagccat ccgaccatgt gggaaacgcc
     7261 tgctcaacga aggtgccatc gatggtgacg aggaattcgg tcacgttatt gccctcgaag
     7321 gcgccgaccg tcaagcactt gcgctcgatt ttggctttgg gtgaagggag cactcgcgtg
     7381 cctcccgtga tggccagggg gcagccgtcg cgatcgctcc ccgacgccag cgtcagcatc
     7441 ggagtgaccg tcgtaagcgg cctcacgatc tgtgaggaaa acgttgtaaa gcaggttccg
     7501 tcattccagc atgcggagaa cggtgccttg ccagagaagt tcacgtaggc cgagtcgacc
     7561 agagcacagc cgttcccgga tgcccacact tgcgccgtag ggatcaatgg cggcaccacg
     7621 gccacacctg tctgcgtacc atcgcagacg gcgtccttga acgttgcgat ccggtaatcg
     7681 ccgggaatct ggacgtggcg cacgagggaa gtttcgttgc tgaagaagtg ctccccgtcc
     7741 gaccacactc cttgaaacgg aggggtacct gtgaaggtga cgttcatgag cgccgagccg
     7801 cacgagagag ggccggagct gacgatggct ttcggattgt cacagggggg tttcgggacg
     7861 atggcgaagc tgcgctcggc cgcgctcatg caggcgccgc gcgtgaagcg cacagccagg
     7921 ccgacgctgc cgctcgatcc tgccgtgacc gtcactgagt cgcctgtcgc ggtcgccggc
     7981 gtgccattcg tgatcgtcca cgaggccacc gcaccgggct gcggacgcac ggacgccgaa
     8041 aagactgttt cgggaatgac ggaatccggc ccttcgatgg ccgccggcgg attggagcag
     8101 actccgatcg tgtacggcat cgcggcgtgg gcggtggaac aggcggaggc gttgacggga
     8161 ctcttcgcga cagcgcg
//
LOCUS       DATLVW010000042         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_291301, whole genome shotgun sequence.
ACCESSION   DATLVW010000042 DATLVW010000000
VERSION     DATLVW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291301"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNN25_01505"
     CDS             complement(<1..432)
                     /locus_tag="VNN25_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXH90227.1"
                     /translation="MNVKTDRLAGLWRSSVPLFVSAGFWVLNRLAYSRKRIVVYGLDD
                     VRPTILEGSRFLALAALIIFVVRLVDAVVFDLIMRRRRDVVAPQLLRQMVSIAVYLVL
                     FASALKTFFDYDVRTALTTGAVLAAVIGLALQDTLGNLFGGI"
     gene            complement(429..671)
                     /locus_tag="VNN25_01510"
     CDS             complement(429..671)
                     /locus_tag="VNN25_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90228.1"
                     /translation="MSVPLFFALYCLEAGLFFIIVPWTRVWSMNSLLHATPALILLST
                     NPFVRGFVSGFGVAHLLVGVKDIMRIAAGRKDGRPE"
     gene            complement(779..3049)
                     /locus_tag="VNN25_01515"
     CDS             complement(779..3049)
                     /locus_tag="VNN25_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HXH90229.1"
                     /translation="MPNAAGMVQVNEENAMRKSTLLLLLFAAVIALPLYARDRSHSYI
                     TYDDGGTVIRQAEDGREVDARVNMPVFPGDEVTTSRRGRLEIRMADGNIIALDRSTTV
                     FFKSILDSYDGDSDQTIVELRSGHVAIQRDEETRELLRLDTPNASYAASEAAIYAVEA
                     DAKTQDRVTVFDGTMEVRTPSRTTRVRQGEVAHVDGEGMYDLLGSHDAADEFERWFLQ
                     RASRYNTASSRYLDRSLAYSEADLGSSGAWFFAANFGGWCWRPHVAAGWRPYYNGYWR
                     HSPGGVLVWVSYDPWGWVPYHYGRWAYQPAYGWVWLPGAAYAPAWVYWMYSPSVIGWA
                     PMGWYDCYGPYYDWAYRPYSRAGFIGGGFRGRIRPGEVDLRPWTFVTPETLMHRHIDQ
                     ASLAGDIVRDRLHRDGDSGLVTNSPARFTRNELRDPNTAIGNVFKHGIHGGAAGGAGK
                     DGLAADMTPFFRRDPQLPNSIRERVVHAQPVDGAVGTGLTGGHTGAPSGVPTPGTSGT
                     LEGQITRGADGNVPRSGITPGIIHDGRVVGGHDDVPNGGLRRGDTPSGATATPVSAGS
                     SSTNDWRRGRAVDGGLRPGAGVQAPSPDANRREVQPGDHGAIDKGKTNGNDSGLHHDS
                     PPSNEVSPNVTPVPPPDNWRGRAVGRHENSSGPSAPPPVGDANKSDVPRRIIDGVGGP
                     RISHGDGPANDSPKHDRSTHSSEPSSSSGQGSRDSGRSTERSSPPSSQPSSTERSHDS
                     GSHESPKAESGHGKKD"
     gene            complement(3159..3899)
                     /locus_tag="VNN25_01520"
     CDS             complement(3159..3899)
                     /locus_tag="VNN25_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXH90230.1"
                     /translation="MRALVVSDLHSNAEALRVVMNRVRRKKFDRVICLGDFVGYGAQP
                     NQVLDTMRTMKMPKLYIRGNHDRVAAGLDQGEGFNAVAKTAAIWTRDHLSARNRHFLV
                     KLPLGPLEQDGVLICHGAPHNEDEYVFHEGHARDIFAESTAPFILYGHTHLPVIFSID
                     RMHRVKGVAIRGDVVVRLDHDHRYLINPGSVGQPRDRNPDSSFVIFDSKRLTVQFFRV
                     AYDVAKTQASILRAGLPEILAQRLAYGA"
     gene            complement(3896..>4180)
                     /locus_tag="VNN25_01525"
     CDS             complement(3896..>4180)
                     /locus_tag="VNN25_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXH90231.1"
                     /translation="QFIQSEGLSVYLSAPYSLLRGRIGDKAAERPLFRDDAAAHELYN
                     NRIRYYRMSDITIEIREEETTNEIVERLLLELPKGFLDPGRRPPAGGTRS"
BASE COUNT          778 a         1294 c         1387 g          721 t
ORIGIN      
        1 aatgccgcca aacagattgc cgagtgtgtc ctgcagcgcc aggccgatga ctgccgcaag
       61 aaccgcgccg gtcgtgagcg ccgtcctgac gtcgtagtcg aagaaggtct tgagcgcgct
      121 cgcaaaaaga acgaggtaga cggcgatgga gaccatctgc cgaaggagct gcggcgcgac
      181 cacgtcccgc cgccgccgca tgatcagatc gaaaacaacg gcgtcaacca gccggaccac
      241 gaagatgatc agcgcggcca gcgcaagaaa gcgggaacct tcgagaatag tcgggcgcac
      301 atcgtcgagc ccgtagacca cgatcctctt gcgagagtaa gccagccggt tgagcaccca
      361 gaaaccagcg ctgacgaaga gcggaacgga agagcgccag aggccggcca gccggtccgt
      421 cttgacgttc actccggccg cccatccttg cggccggcag cgatccgcat gatgtctttg
      481 actccgacca gaaggtgagc cacgccgaag ccggagacga agccgcgaac gaacgggttc
      541 gtggataaga ggatcagcgc cggcgtcgcg tgcagcagcg agttcatgct ccagacccgc
      601 gtccaaggca cgatgatgaa aaagagccct gcctcgagac agtagagggc aaagaagagg
      661 ggaacggaca tcggggggat tgtagcggaa ggttcgcgca gggccgcggc ggggattggc
      721 gatccgctac ttcaaaggag ccggagagcg gcagcatggc cgccgctctc cggaagactc
      781 agtctttctt gccgtgtccg ctttcggctt tcggcgactc atgcgatccg ctgtcgtgag
      841 agcgttcggt gcttgacggc tgtgacgatg gcggggacga acgctccgtg ctgcgaccgg
      901 aatcgcggga gccttggccg ctgctcgagc tcggctccga ggagtgagtc gatcggtcgt
      961 gcttcggtga gtcgttggca ggcccgtctc cgtgtgagat gcgtgggccg ccgacgccgt
     1021 cgatgatgcg gcgcggcaca tcgctcttgt tcgcatcgcc gaccggcgga ggcgctgacg
     1081 gcccgctgct gttctcatga cgcccaaccg cgcgcccgcg ccagttatcg ggcggcggaa
     1141 cgggcgtcac attcggtgac acttcattgg atggcggcga gtcgtgatgc agaccgctgt
     1201 cattcccgtt cgtttttccc ttgtcgatcg cgccatgatc gccgggctga acttcgcgcc
     1261 ggttcgcgtc cggcgacgga gcctggacgc cagcgccggg tcgcagaccg ccgtcgaccg
     1321 cacgcccgcg gcgccagtca ttggtgctgc tgcttccagc ggaaacaggc gtggccgtcg
     1381 ctccactcgg agtgtcgccg cggcgcaaac cgccgttggg aacatcgtca tgtccgccga
     1441 caacacgtcc gtcatgaatg atgcctgggg tgattccgga acgtggcaca ttgccgtccg
     1501 cgccgcgggt gatctggcct tccagggtac cggacgtgcc cggcgtagga acgcccgaag
     1561 gcgcgccggt gtggccgcca gtcaagccgg tgcccacggc cccgtcgaca ggctgcgcgt
     1621 gtacgacgcg ctcgcgaatc gagttgggaa gctgagggtc gcggcggaag aacggcgtca
     1681 tgtccgctgc cagaccatcc ttgcctgcac caccggctgc gccaccatgg attccgtgtt
     1741 tgaagacgtt gccgatggcg gtgttcggat cgcggagctc atttctcgtg aaccgggccg
     1801 gcgagttggt caccaggccg ctgtcgccgt cgcggtgcaa ccggtcacgc acgatgtcgc
     1861 ctgcaagcga agcctgatcg atgtggcgat gcatcagcgt ttccggcgtc acgaacgtcc
     1921 aggggcgaag gtcgacctcg ccggggcgga tgcgtccgcg gaagccgccg ccgatgaatc
     1981 cggcgcgcga gtacggacgg tatgcccagt catagtacgg gccgtagcag tcataccaac
     2041 ccatcggcgc ccagccaatg actgacgggc tgtacatcca atagacccac gccggcgcat
     2101 acgccgcacc ggggagccac acccacccgt acgccggttg gtaggcccag cggccgtagt
     2161 ggtagggaac ccatccccac ggatcgtacg aaacccacac gagaacgccg cccgggctgt
     2221 gccgccagta accgttgtaa tacggacgcc agccggcagc gacatgcggg cgccagcacc
     2281 agccgccgaa gttcgcggcg aagaaccagg cgccgctcga cccgaggtcg gcttccgagt
     2341 aggccaggga acgatcgaga taacggctgc tggcggtgtt gtaccgcgag gcgcgctgca
     2401 ggaaccaccg ctcgaactcg tccgcggcgt cgtgtgagcc gaggaggtcg tacatccctt
     2461 cgccgtcaac gtgcgcaacc tcgccttgcc gcacgcgcgt cgtgcgagac ggcgtgcgca
     2521 cttccatcgt gccgtcgaac acggtcacgc ggtcctgggt ctttgcatcc gcttcgacgg
     2581 catagatcgc tgcctcgctg gcggcgtagc tggcgttcgg cgtatcgagg cgcagcaact
     2641 cgcgcgtctc ttcgtcgcgc tggatggcga cgtgaccgga gcgaagctcg acgatcgtct
     2701 ggtcggagtc gccgtcgtac gagtcgagga tcgatttgaa aaagacggtc gtggagcgat
     2761 cgagggcaat gatgttgccg tcggccatac ggatctcgag gcggccgcgc cggctcgtcg
     2821 tgacctcatc gccggggaag accggcatgt tgacgcgcgc atcgacttcg cggccatcct
     2881 cggcctggcg aatgacggtt ccaccatcgt catacgtgat gtacgaatgg ctgcggtccc
     2941 gcgcgtacaa cggaagagcg atgaccgctg cgaaaagaag aagaaggagt gtggacttgc
     3001 gcattgcgtt ttcctcgttg acctgcacca ttcctgctgc gtttggcatg ccacgcaacg
     3061 attgtcgatt cgctcctgtt ttcagcgact tactacggtt tagacacatg caccaaacgc
     3121 tcgtctcctc gatcgaggac agcgccgtca ccggacggtt acgctccgta ggcgagacgc
     3181 tgggcgagga tctccggcag acccgctcga aggatcgacg cctgcgtttt cgcgacgtcg
     3241 taagcgacgc ggaagaactg gaccgtaagg cgtttcgagt cgaagatcac gaacgacgaa
     3301 tcgggattgc gatcgcgcgg ctgcccaact gagccgggat tgatcaggta gcgatgatcg
     3361 tgatccagcc gcacgacgac gtcgccgcga atggcaacac ccttcacacg gtgcatgcgg
     3421 tcgatcgaga agatcacagg caggtgtgtg tggccgtaga ggatgaacgg tgccgtgctc
     3481 tcggcgaaga tgtcgcgggc atggccctcg tgaaaaacgt attcgtcttc gttatgagga
     3541 gcgccgtggc agatcagcac tccgtcctgc tccagtgggc caaggggtaa cttcacgaga
     3601 aagtggcggt tgcgcgcgga gagatgatcg cgcgtccaaa tggccgcggt cttggcgacg
     3661 gcgttgaagc cctcgccctg atcgaggccg gcagcgacgc ggtcatgatt ccctcggatg
     3721 tagagcttcg gcatcttcat cgtccgcatc gtgtcgagca cttggttggg ctgcgcgccg
     3781 tagccgacga aatcacccag gcagatcaca cggtcgaact tcttccggcg aacgcggttc
     3841 atcaccacgc gcagcgcctc ggcgttcgag tgcagatcgc tgacgacgag tgccctcatg
     3901 aacgagtgcc tcccgccggt gggcggcgcc ccggatcgag aaagcctttc ggcaattcca
     3961 gcaggaggcg ctcgacgatt tcgttcgtcg tttcctcctc gcggatttcg atcgtgatgt
     4021 cggacatgcg gtagtagcgg atgcggttgt tatacagctc gtgcgcggca gcatcgtcgc
     4081 gaaaaagcgg ccgctcggcg gctttgtcac cgatgcggcc acgcaagagc gagtatggtg
     4141 ccgaaaggta caccgaaagc ccctcgctct ggatgaactg
//
LOCUS       DATLVW010000043         7575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_333739, whole genome shotgun sequence.
ACCESSION   DATLVW010000043 DATLVW010000000
VERSION     DATLVW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333739"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1444
                     /gene="ispG"
                     /locus_tag="VNN25_01530"
     CDS             299..1444
                     /gene="ispG"
                     /locus_tag="VNN25_01530"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HXH90232.1"
                     /translation="MKARVTRKVAIGNITIGGDSPIAVQSMTATKTQDVAATIRQVEL
                     LEAAGADVIRIAIDTAKDVTALAEVRAARPNANLVIDLQENYRLAERVAPFVQKLRYN
                     PGHLYHIERDKPIRDKVAYLVDVARAHGCAIRIGVNAGSVDPEQLDRYPKEDSISPMI
                     DSALEHCLMLDDFGFDQYVVSLKDSDPNKVIDANIRFAAARPDVPLHLGVTEAGMPPD
                     GVIKTRVAFEQLITRGIGDTLRVSLTLPFEDKGQEITVGRKILEDIANGRLRSVARFE
                     QKGMNIISCPSCSRVENEAFIDLAQNVKEMTRYANEHDITIAVMGCRVNGPGETDDAD
                     LGMWCAPTYVNLKRGPETLGHYTYDEILGRLRVELDKLIEEKRGVAV"
     gene            1441..1938
                     /gene="lspA"
                     /locus_tag="VNN25_01535"
     CDS             1441..1938
                     /gene="lspA"
                     /locus_tag="VNN25_01535"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXH90233.1"
                     /translation="MKRIPYLVLSVIVILADAWTKFLVSSRIDLHESIPLIPNFLQLV
                     HVRNTGAAFGIGANADSRFVPLLLNLGAIGVFFVVVAYAFRAAVTDRVLQTGLHLILG
                     GAIGNLIDRFRLGYVVDFVDVFVKSHHWPAFNVADSAICIGIGLLFLDMRKKPDEVTA
                     AEPAA"
     gene            2083..2700
                     /locus_tag="VNN25_01540"
     CDS             2083..2700
                     /locus_tag="VNN25_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH90234.1"
                     /translation="MPRQLIPHNEYQKLKIRSRGYLPHWEIEGATYSITHRLIDSLPI
                     EVTRQLSLEKQAMERQITGGIRALTVIERSRIGDAFALRLDDELHAERGACHLRNSKL
                     ADLVAGNLQYFNGRRYELYSWCVMSNHVHTVVKPFAGECLEEILHSWKSYTAHKATAI
                     VGTRKLWAREYFDRMIRDERHLADAISYVRNNPAKAGLVNWKWVG"
     gene            complement(2782..3714)
                     /locus_tag="VNN25_01545"
     CDS             complement(2782..3714)
                     /locus_tag="VNN25_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXH90235.1"
                     /translation="MFPVAAIASQPRVRPQGPRVNIVGVAVLVLPLVTGVAVTIATEN
                     PFGVIGGLLLGLVLMQSPKVAKQWEKAVVLRLGKFIGLRGPGLFWIVPFIDNVSSWID
                     QRVITTSFAAEQTLTSDTVPVNVDAVLFWMVYDAEKAALEVQQYQDAVSWAAQTALRD
                     IIGRTSLGDLLRGREKIEELLQELIDGRCNPWGVTVQSVEMRDVIIPDALQDAMSREA
                     QAAREKAARIILGEAEVEIAKLFQVASVSYHDNPTALHLRAMNMLYEGLKEKGAMMIV
                     PSTAVESMGMGGLMGMAAMAQRQLTESAPAEPAA"
     gene            complement(3714..4103)
                     /locus_tag="VNN25_01550"
     CDS             complement(3714..4103)
                     /locus_tag="VNN25_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXH90236.1"
                     /translation="MAAPITIDANDRTPIYAQLERGIRVAIATGRMRAGDQLPTVRQL
                     AVDLRVNANTVAKVYLALEREGVVVTRRGVGTFIAESVPKASHAGHRERRLAAHIDRF
                     LTDSAALGYSPREAMQQIARRLKEGES"
     gene            complement(4235..5560)
                     /locus_tag="VNN25_01555"
     CDS             complement(4235..5560)
                     /locus_tag="VNN25_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH90237.1"
                     /translation="MRLTRTIALHLIATIAVIAIAAAFGANVFFHASQRGATFFGAAL
                     FLLGMAVIALTMRAPLRRIRRNLSALVDGVRGFADDDFSLRLHVTGHDEITDLVAIYN
                     EIGNILRDQRNESVQRELLFETVLQASPLSMILTSNHGRIVFVNRAARDLFGAGDRIE
                     GRRLDELADEVPALRDALRATGGALFTVDGETFQLSQRTFYLNTREHVLYLIARLTQD
                     LRRQELDIWKRAIRVVNHELNNSLAPIHSLFHSANVVRDRPGDSARLVEINAAIEERL
                     ASLQQFIDGYAHLARLPKPRRERVVWSELLNAVLPLAPFVIAEPLPADECSFDRTQIE
                     QVLLNLVKNAAEAGSEEREIVVSVVRRGNASVLTVQDRGRGMDDETMNRALLPFYSTK
                     PGGTGLGLALVGEIVDAHGGNVRISRRDGGGISITCLIPDGGVAESSSS"
     gene            complement(5646..7025)
                     /locus_tag="VNN25_01560"
     CDS             complement(5646..7025)
                     /locus_tag="VNN25_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH90238.1"
                     /translation="MASTHGHGRIDGTVLIVEDQPAVAKALLLLFDIHGIDAIAVSNP
                     ADAEEVIRRGDADAVVQDMNFTPGATGGEQGLALFRSVRAIDPGMPVILLTAWTALET
                     AVQMVKEGAIDYLAKPWSDDKLLAIVGNALTERRGERDVRRSRSALAASVDLCGVVYE
                     STAMHRIVTLALQVAGADVPVLITGPNGTGKEKIADLIQANSRRRKAPFVKVNAGALP
                     DELLESELFGAEAGAFTGSKRLRIGRFETANGGTLFLDEIGNLSSAGQTKLLRVLQNG
                     EFERLGSSDTHRVDVRLLCATNADLAQAVRSGAFREDLFFRLNVIEIHVPPLRERRED
                     VLPIAESFLVAARKSSGRAWRLGADALAALTAYDWPGNIRELQNRIQRATVTAAGDIL
                     SAADLGLSTDRPPTPAPVHGEKEQIENVLREAGGNVSDAAKDLGVSRQALYRKMQRLG
                     IVLERRPRS"
     gene            complement(7395..>7575)
                     /locus_tag="VNN25_01565"
     CDS             complement(7395..>7575)
                     /locus_tag="VNN25_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH90239.1"
                     /translation="VAAAYGLNVLLVTNAAGAKLDWRFVVAAVVLLWLQGIVATLIPA
                     MRATRFSPVIATRGA"
BASE COUNT         1401 a         2395 c         2374 g         1405 t
ORIGIN      
        1 gggattggtg ccgccgacct ggatgatcac tggcggcgtg caggtctgcg cttgcacgac
       61 acctggcatc gccagcgtca ggatcagaag cactgcgttg gtgaagcggg acatgagacc
      121 tccggaggcg tcacaggagc aacgcgaaag gctatttatt tgcgtaatgg aagataggtg
      181 gggccaccgg aacttatccg atgccgagca cggctgcaat aggtttatgc cgaacgaatg
      241 ttcggtcttg gactcccacg cggaagggtt tgagccctcc ccgcgttcac gacacccgat
      301 gaaagcacga gtcacccgta aggtcgctat cggcaacatc accattggtg gtgacagccc
      361 catcgccgtg cagtcgatga cggccacgaa gacgcaggac gtcgccgcca cgatccggca
      421 ggtggaactg ctcgaggcgg ccggcgctga cgtgatccgc atcgccatcg ataccgccaa
      481 ggacgtcacc gcgcttgccg aagtacgcgc cgcccggccc aacgccaacc tcgtcatcga
      541 tcttcaggag aactaccgtc tggccgagcg cgtcgcgccg ttcgtgcaga agctccgcta
      601 caaccccggc cacctctacc acatcgagcg cgacaaaccg atccgcgaca aggtcgccta
      661 cctcgtcgac gtcgcacgtg cgcacggctg cgcgatccgc atcggcgtca atgccggctc
      721 ggtcgatccc gagcagctcg acagatatcc gaaagaagac tcgatctcgc cgatgatcga
      781 ttccgcgctc gagcactgcc tgatgctcga cgacttcggc ttcgatcaat acgtcgtgtc
      841 gctcaaagac tccgatccaa acaaggtcat cgacgcaaac atccgtttcg ccgctgcccg
      901 tcccgacgtt ccgcttcacc tcggcgtcac cgaagcgggc atgccgccgg acggcgtgat
      961 caagacccgc gttgccttcg aacagctcat cacccgcggc atcggcgaca cactccgcgt
     1021 ttcgctcacg ctgcccttcg aagacaaagg tcaagagatc accgtcggcc gcaagatcct
     1081 cgaagacatc gccaacggcc gcctccgctc cgtagcgcgt ttcgagcaga aggggatgaa
     1141 catcatcagt tgcccttcct gctcgcgcgt cgagaacgaa gcgttcatcg atctcgcgca
     1201 gaatgtcaaa gagatgacga ggtacgcgaa cgagcacgac atcacgatcg ccgtcatggg
     1261 ctgccgcgtg aacggacccg gcgagaccga cgacgccgac ctcggcatgt ggtgcgcgcc
     1321 aacgtacgtc aatctcaagc gcggcccgga gacgctcggc cactacacgt atgatgagat
     1381 tctcggccgt ctgcgtgtgg agctcgacaa attgatcgaa gagaagcgag gcgtcgcggt
     1441 ttgaaacgaa tcccctatct cgttctctcc gtcatcgtga tccttgccga cgcctggaca
     1501 aagtttctcg tctcgtcgcg catcgatctt cacgagtcga ttccgctgat ccccaacttc
     1561 ctgcaactcg ttcacgtgcg caacaccggc gctgccttcg gaatcggcgc gaatgccgac
     1621 tcccgtttcg ttccccttct cctgaacctc ggcgccatcg gcgtcttctt cgtcgtcgtc
     1681 gcttatgcct ttcgcgctgc ggtgaccgac cgagtgctcc agaccggact gcacctgatt
     1741 ctcggtggcg ccatcggcaa tctcatcgac cgcttccgcc tcggctacgt agtcgatttc
     1801 gtcgacgtct tcgtaaagtc gcaccactgg cccgccttca acgtagccga ctccgccatc
     1861 tgcatcggca tcggcctcct cttcctcgac atgagaaaga agcccgacga agtcaccgcc
     1921 gcggagccgg cggcgtaacc tggtgtagcg ccgtagacaa gcgtagcgcc gtagacaagc
     1981 gtagcgccgt cggctcgacg gctgttccgc cgccggcccg gcggcgacct ttccgaggta
     2041 cacttcccac tcccacaccg catcgcgcgt gctccgcgcg aaatgccacg tcaactaatc
     2101 ccacacaacg aataccaaaa actaaagatc cgctcccgcg gttatctccc gcattgggag
     2161 atcgagggtg cgacctactc gatcacccat cggctcatcg attcgcttcc catcgaagtc
     2221 acacgccagc tctcgttaga aaagcaggcg atggagcgcc agatcaccgg cggaatccgc
     2281 gcacttactg tcatcgagcg aagccggatc ggggatgcct ttgcgcttcg tctcgacgat
     2341 gagctgcatg cagagcgagg agcgtgtcat ctgcgaaatt cgaaactagc ggatctcgta
     2401 gccggcaacc tgcagtactt caacggacgt cgatacgagc tttactcgtg gtgcgtgatg
     2461 tcgaatcacg tccacaccgt ggtcaagcca ttcgcaggcg agtgcctcga ggagattctc
     2521 cattcgtgga agtcgtacac ggcgcacaag gcgactgcga ttgtcggaac gaggaagctt
     2581 tgggcgcgtg agtattttga tcgcatgatt cgcgatgagc ggcatctggc ggatgcgatc
     2641 agctatgtac ggaacaatcc tgcgaaggcg gggttggtga attggaagtg ggttgggtga
     2701 gagtcgccgg cgagccgccg gcggtacagc cggcgggccg ccggcgctac gctacacaca
     2761 cttgcggccc gccggtttct tctacgctgc cggttccgct ggcgcggatt cggtcaactg
     2821 gcgttgggcc atggcggcca tgcccattag gccgcccatg cccatcgact ccactgcggt
     2881 cgacggcacg atcatcatcg cgcccttttc tttcagtcct tcgtacagca tgttcatcgc
     2941 gcggaggtgc agggcggttg ggttgtcgtg atacgagacc gaggccacct ggaagagttt
     3001 ggcgatctcc acttctgctt cgccgaggat gatgcgggcg gctttctcgc gcgcggcttg
     3061 cgcttcgcgc gacatcgcgt cctgcagcgc gtcgggaatg atcacgtcgc gcatctccac
     3121 cgactgcacg gtcacgcccc acggattgca gcgaccgtcg atgagctcct gaagcagttc
     3181 ttcgatcttc tcgcgtccgc gcagcagatc gccgagggac gtgcgtccga tgatgtcgcg
     3241 cagtgcggtc tgcgcggccc agctcacggc atcctgatac tgctgcactt cgagcgctgc
     3301 cttctcggcg tcgtacacca tccagaagag aacggcgtcg acgttgaccg gcacggtgtc
     3361 cgaggtcagc gtctgctcgg cggcgaagct cgtcgtaatc acgcgctgat cgatccacga
     3421 cgacacgtta tcgatgaacg gcacgatcca gaagagtccg ggcccgcgca gtccgatgaa
     3481 cttgccgagg cgaagaacga ccgctttctc ccactgcttg gccaccttcg gcgactgcat
     3541 cagcacgagg ccgagcaaaa gaccgccgat gactccgaag ggattctccg tggcgatcgt
     3601 gacggccacg ccggtcacga gcggcagaac cagaacggcc acgccgacga tgttgacgcg
     3661 cggcccctga ggacgaacac gtggttggct ggcgattgct gcgactggaa acattagctt
     3721 tctccttcct tcagtcgccg cgcgatctgc tgcattgctt cgcgcggcga atacccgagg
     3781 gctgcggaat cagtgaggaa gcggtcgatg tgcgcggcaa ggcgtcgctc gcgatgtcct
     3841 gcatgcgacg ccttcggcac cgactcggcg atgaacgtcc cgacaccgcg ccgcgtcacg
     3901 acgacgccct cgcgttcgag cgcgaggtag accttggcga cggtgttggc gttcacgcga
     3961 aggtcgacgg cgagctggcg gacggtcggc aattggtcgc cggcccgcat ccgtcccgtg
     4021 gcgatggcga cgcgaatgcc gcgctcgagt tgcgcgtaga tcggcgttcg gtcattggca
     4081 tcgatggtga tgggtgcggc cacgctgtcc tcttgtacta cgactttagt acactacgga
     4141 gaaaggtgaa tagggtttcc gatccggggt tagtgcgtac ggaatgagcg cttgcaaccg
     4201 gtaagcgggc ggcggctcac gtctaaggat gaagttacga cgaggatgac tccgccactc
     4261 ccccatccgg gatcaagcaa gtaatcgaga ttccgccacc gtcgcggcgg ctgatgcgaa
     4321 cattccctcc gtgcgcgtcg acgatctcgc cgacgagggc gaggccgagt ccggtaccgc
     4381 cgggcttggt tgagtagaac ggcaggaggg cgcgattcat tgtctcgtcg tccattccgc
     4441 ggccgcggtc ctggacggtg agcacgctcg cgttgcctcg ccgtacgacg ctcaccacaa
     4501 tctccctctc ctcgcttcca gcctcggcgg cgttcttgac gaggttgagg agcacctgct
     4561 cgatctgcgt gcggtcgaac gaacactcgt ccgccgggag cggctcggcg atcacgaacg
     4621 gggcgagcgg aaggacggcg ttgagcaact cgctccagac gacgcgctcg cggcgcggct
     4681 tcggcaaccg cgccaggtgg gcgtagccgt cgatgaactg ctgcagcgag gccagccgct
     4741 cctcgatcgc ggcgttgatc tcgacgagcc gtgcgctgtc gccggggcgg tcgcggacga
     4801 cgttcgcgga atgaaaaagc gagtggatcg gcgcaagcga gttgttcagc tcgtgattga
     4861 cgacgcggat ggcccgcttc cagatgtcga gctcctgccg gcggagatcc tgcgtcaggc
     4921 gggcgatgag gtagagaacg tgctcgcgcg tattgagata gaacgtgcgc tggctgagtt
     4981 ggaaggtctc gccatcaacg gtgaacaacg cgccgccggt tgcacgcagt gcgtcgcgca
     5041 gtgcgggcac ttcatcggca agctcgtcga ggcggcgtcc ttcgatgcgg tcgccggcgc
     5101 cgaaaagatc gcgggccgcg cggttgacga agacgatgcg gccatggttg ctcgtcagaa
     5161 tcatcgatag cggcgatgcc tgcaagacgg tctcgaagag cagctcgcgc tggaccgatt
     5221 cgttgcgctg atcgcgcagg atgttgccga tctcgttgta gatggcgacg aggtcggtga
     5281 tctcgtcgtg tccggtgacg tgcagacgaa ggctgaaatc atcgtcggcg aacccgcgaa
     5341 caccgtcgac gagcgcgctg aggttccggc ggatgcggcg tagcggtgcg cgcatggtga
     5401 gggcgatgac ggccatgccg aggaggaaga gtgccgcgcc gaagaacgtg gcgcctcgct
     5461 gcgaggcgtg aaagaaaacg ttggcgccga aggcggccgc gatagcgatc acggcgatcg
     5521 tggcgatgag atggagcgcg atcgttctcg tgaggcgcat ttgcgagaga aacaattaac
     5581 cacagagaca cagaggacac agagatgaaa ttctctgtgc tctctgtgtc tctgtggtta
     5641 aggtctcacg atcttggccg ccgctccagc acgatgccga ggcgctgcat ctttcggtac
     5701 agcgcctggc gcgagacgcc gaggtccttc gcggcatcgg agacgttgcc tccggcctcg
     5761 cgaaggacat tctcaatctg ctccttctcg ccgtgaacgg gagcgggggt gggcgggcgg
     5821 tcggtggaga ggccgaggtc ggcggcggag aggatgtcgc cggcggcggt gacagtggcg
     5881 cgctggatgc ggttctgcag ctcgcggatg ttgcccggcc agtcgtatgc ggtgagcgcg
     5941 gcaagcgcgt cggcgccgag ccgccatgcg cggcctgagc tcttgcgcgc ggcgaccagg
     6001 aacgactcgg cgatcggaag cacgtcttcg cggcgctcgc gcagcggcgg cacgtgaatc
     6061 tcgatcacgt tgaggcggaa gaagagatcc tcgcggaacg ctccgctgcg aacggcttgg
     6121 gccagatcgg cgttcgtcgc gcagaggaga cggacgtcga cacggtgcgt gtcgctgctg
     6181 ccgaggcgct cgaactcgcc gttctgcagt acgcgcagta gcttcgtctg accggcggat
     6241 gacaagttcc caatctcatc gagaaacagc gtcccaccgt tggcagtctc gaaccgtccg
     6301 atgcgcagcc gtttcgatcc cgtgaacgcg cccgcctcgg cgccgaataa ttcgctctcg
     6361 agcagttcat cgggcagcgc gccggcgttc accttaacga atggcgcttt gcgtctccgc
     6421 gagttggcct ggatcaggtc cgcgatcttt tccttccccg tgccgttcgg tcccgtgatc
     6481 agcaccggca cgtcggcgcc ggcgacctgc agtgccagcg tgacgatgcg gtgcatggcc
     6541 gtgctctcat agacgacgcc acagagatcg acgctcgcgg ccagcgcgga acgggagcgt
     6601 cgcacatcgc gctcgccgcg ccgttcggtc agcgcgttgc cgacgatggc cagcagtttg
     6661 tcgtcgctcc acggtttggc gagatagtcg atcgctcctt ccttcaccat ctgcaccgcc
     6721 gtttcgagcg cggtccaggc ggtgagaagg atcaccggca taccgggatc gatcgcgcga
     6781 acgctgcgga agagcgccag cccctgctcg ccaccggtcg ctcccggcgt gaagttcatg
     6841 tcctgcacga cggcgtcggc gtccccgcgt cggatcacct cttcagcatc ggccggattc
     6901 gagacggcaa tcgcgtcgat gccgtggata tcgaacagta gcaacagcgc tttcgcaact
     6961 gccggctgat cttcgacgat caggaccgtc ccgtcgatgc ggccgtgtcc gtgcgtcgaa
     7021 gccatgcttg cctgttggtg cgttccaatc aattgatgct cccgatcaga gagaagagat
     7081 tagccggatt tctctcactt ccgcactact ctccaatcga gcgccttctc ccccacgctt
     7141 tttgtggggg agaaggtggc gaagccggat gagggggcat ctgcgcaatg aatctgtttt
     7201 cccgcacttt gcaacgcccc cctcatccgc ctccggcacc ttctcccccc gaaaagcgcg
     7261 gggggagaag gcgctcgatt gaaaaggagt cgcagaaatg tgtgagaaat gcgggtcagc
     7321 gacaagcgat ccggaacact ctgcgacatc accctgatct ctgatctctg atctctcgtc
     7381 tctaatctct gacttcatgc tccgcgtgtt gcaatcaccg gcgagaagcg cgtggcacgc
     7441 atcgccggaa tcagcgtcgc cacgataccc tgcaaccaga ggagcacgac ggcggcgacg
     7501 acaaatcgcc aatcgagctt cgcgccggcc gcattcgtga ccagcaggac attcagtccg
     7561 taggctgcgg cgacg
//
LOCUS       DATLVW010000044         7303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_456842, whole genome shotgun sequence.
ACCESSION   DATLVW010000044 DATLVW010000000
VERSION     DATLVW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456842"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="VNN25_01570"
     CDS             <1..1721
                     /locus_tag="VNN25_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HXH90240.1"
                     /translation="SGWGSGFRRHVAENAALLNLSRGGRSSKSYTAEGHWADALRRHP
                     TYILVQFGHNDMPGKGLDRETTIPAFRANMARYVDEARAAGAKPILVTPLTRRYFGVD
                     GRIHSDLGDYAEATRSVAAEKNVPLIDLQARSIELLDRLGPSISSSLGPLKPDGTLDV
                     TLDKTHLNEAGSALFGALMAEEVQRVVPELAPFIRAVVLPAPVPLWSSRMAESVMKRT
                     PDPLLLDSDKPKWDYTQGLVLKAMFDLAQRTGDERYRKYATAWYDGMIDADGTIHGYR
                     LDEYSLDRINPGKPLFALYEKTHDEKYRKAIETLRRQLREQPRNADGGFWHKKRYPHQ
                     MWLDGLYMAAPFLAQYGKVFHEPAAFDDVITQFVLMEKHARDESTGLLTHGWDESRLQ
                     KWADPKTGRSPAFWGRAMGWYAMGLVETLDFIPNDHPRRGELIAILQRFVEAITRVQD
                     PKSSVWWQVVDQPGREKNYLESSASAMFTFVLLKSSRLGYIDAKYAAVGRRAYDGLLK
                     EFVEVDKDGLVTIHRAVQVVGLGGDPERERYRDGTYEYYVTEKVRDNDPKAVGPFIFA
                     SMEMER"
     gene            1798..1977
                     /locus_tag="VNN25_01575"
     CDS             1798..1977
                     /locus_tag="VNN25_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90241.1"
                     /translation="MLLLRALSHGGPHLSDRDGFASGKPLKHAEATIQGRRLLAFERQ
                     QPIAANRYIEPVTLL"
     gene            complement(2210..3151)
                     /locus_tag="VNN25_01580"
     CDS             complement(2210..3151)
                     /locus_tag="VNN25_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90242.1"
                     /translation="MAQSEWAPDALGAHRGNAGPVHHVVFDHCSATWAIDENLSASGP
                     ADMDSTRDPDVTSHDITLRFCLIAEGLSHATHPKGEHSKGTLVHDGVRNVVIVGCLYA
                     HNRERNPRLKGGTTSIVAGNVMYNWGSACVGVGKHGNRKELQPSETVLVGNVAIAGAD
                     THSQVLVKSVDPGGRVFLRDNVAVAANGAVLPMTDDGVVPLPSPRDLPDIHPWDAAAH
                     VLRSAGARPAHRDPIDARIVRSVIDGTGRIIDSQEQVGGYPVRAQTNRVLSVPDGADA
                     RRKWLESLSNALVDDTTLDLAALWKRVGLLASAEPTR"
     gene            3215..3466
                     /locus_tag="VNN25_01585"
     CDS             3215..3466
                     /locus_tag="VNN25_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90243.1"
                     /translation="MSLDREAPTNQRDAWIGGGLAGDSNPGMPDIEGPALQIDHSSDF
                     EDDEARTRVLQGAAKRAWTVSGERGDPKYPIEPPDSRFG"
     gene            3716..7291
                     /locus_tag="VNN25_01590"
     CDS             3716..7291
                     /locus_tag="VNN25_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH90244.1"
                     /translation="MKQRISLAVLIVLVLILISAPLAAQESRGAIDGIVKDTSGALLP
                     GVTLTVKNVETNVTAMSVSDSKGAYHVSHLNPGTYSVEAGLDGFKTEVRKGIAVHVGA
                     TVTVDFKMEQGALSEVIQVTASTPLLDTSSPATGQVIDSAQIQRLPLGDGTAYMLSRL
                     APGLVDNSDLHFDRPMDNAGLAGIVANGSQGGNDFTLDGAPNKVSPNTSSPGNNSGVV
                     GFSPPSDAIAEFKVQTNAFDAESGHSAGATVNLALKSGTNGVKANVSYFNRDSSRTAT
                     PLLTERADAPKPPRQYNRETSTIAGPIVRDRTFFMVSYEHLRDVNAEPATYTVPTVKM
                     RQGDFSEFSGLIYDPLTATGSNLTRKPFSNNQIPIERINAVAKAYAALYPLPNKPGTS
                     GNYFTNQLRPYDYNAILGRVDHNINDSNKLFIDGYYNKRQEDRYNWALGAANANNGTI
                     NGFDVTHGFDYRSNTGTTLGYTNVRSSNLVFDVRTAWSQFGEWRQAGDQFDPASMGFS
                     PQAVALMNGYKYLPFITFGGFSTTNSGSTIATLGSMRSDWGTGFNRPFTNISFVPSAD
                     WQIYGHSIRTGYELRDQHWKIEVPAYGAGRYNFNGAYTRLNNSAPLNDQAQEWAQFLL
                     GLPTTGTGAVGNSGAVSSQFEIAAKANYSQVSHAFFVQDDWQLNSNLTVNLGVRFELD
                     QAMTEAHNQAISGFDTSISSPIEAAALANYAKNPIAQIAVGDFHVRGGLQFTDGGIYN
                     DLHKVMPRAAFAYQIGDKTVLRAGAGLFSYDYYFDAGNQTGFSQPTAIVTTDNNGATF
                     LANLSNPIPTGGLIQPTGASLGAATGLGLAIGSVTPSQRESPYYRRWQIGGQHDLGAG
                     WKMEVNYLDSRGTHLPVQRELNGIPYQYLSTSNIRDTAQESFLSASVANPFAGMLPGT
                     TLNGATTTRGQLLKPFPEFLSIVTEEYRGSDAYRAASVSLEKRFNQGASLTASYTRSR
                     LTDKLNMLNPSNGILENRVSPNDRPNRATFGGTVDLPFGAGKKWGSNWSGFTQALIGG
                     WSVSATYQYQSGFPLSWGNVYYDPSRNPNDLHSHIGKHVCPGNPNGVAGLDCPAWDTS
                     GFFIAGGTGRTDSRIQLGNNVRTFPSTLSNVRSDPLQLLDVGIYKNFGLPRGMTFQLR
                     IESINALNYTVLFNSNMDPRNAQFGLVNQDRNNPRDIQLGGRLSF"
BASE COUNT         1499 a         2332 c         2114 g         1358 t
ORIGIN      
        1 agagcggatg ggggagcggg ttcagacgac atgtcgcgga gaatgcggcg ttgctgaacc
       61 tctcgcgtgg cgggcggagc tcgaagagtt atacggccga gggacattgg gccgatgcgc
      121 tgcgccggca tccgacgtac atcctggtgc agtttggcca taacgacatg cccggcaaag
      181 gactcgatcg cgagaccacc attcccgctt tccgcgcgaa catggctcgt tacgtcgacg
      241 aggcgcgcgc cgcgggcgcc aaaccaatcc tcgtcacgcc gctcacccgc cgctacttcg
      301 gcgtcgacgg acgcattcac tccgaccttg gcgattacgc cgaagcgaca cggagcgttg
      361 cagcggagaa gaacgtgccg ctcatcgacc tgcaagcgcg gagcatcgag ctgctcgacc
      421 gcctcgggcc ttcgatcagc tcttcgctgg gaccgctgaa accggacggg acactcgatg
      481 tgaccctcga caagacacat ctcaatgaag cggggagcgc gctattcggt gcattgatgg
      541 ccgaggaggt gcagcgcgtc gtgcccgaac ttgcgccgtt catccgcgct gtagtactgc
      601 cggctcctgt gcctctctgg tcatcgcgca tggcggagtc ggtgatgaag cggacacccg
      661 atccgctgtt gttggacagc gacaaaccga agtgggacta cacgcaaggt ctcgtgttga
      721 aggcgatgtt cgatctcgcg caacgcacgg gcgatgaacg ctatcggaag tacgcaacgg
      781 cctggtacga cggcatgatc gacgccgacg gcaccatcca tgggtatcgt ctcgatgaat
      841 acagtctcga tcgcatcaat ccgggcaagc ctctcttcgc gctctacgag aaaacgcacg
      901 acgaaaagta tcgcaaagcg atcgaaacac tgcgccggca actccgcgaa cagccgcgca
      961 atgcggacgg cggtttctgg cacaagaagc ggtatccgca tcagatgtgg ctcgacggct
     1021 tgtacatggc cgcgcctttc ctggcgcaat acggaaaggt gttccacgag ccggccgcat
     1081 tcgacgacgt catcacgcag ttcgttctca tggagaagca tgcccgcgac gaatcgacag
     1141 gcctgctcac gcacggctgg gatgaaagtc gcctgcagaa gtgggcggac ccgaagacgg
     1201 gacgctctcc cgccttctgg ggacgagcga tgggctggta cgcgatggga ctcgtcgaga
     1261 cgctcgactt cattccgaat gatcacccgc gccgcggtga gctcatcgcc atcctgcaac
     1321 gcttcgtcga agcaattacc cgtgtgcagg atccgaagag cagcgtctgg tggcaggtcg
     1381 tcgatcagcc cggccgcgag aagaactacc tcgagtcgtc cgcatcggcg atgttcacgt
     1441 tcgttctgct gaagtcgtcc cggctcggct acatcgacgc gaagtacgcc gcggttggcc
     1501 ggcgcgcgta cgacggtctt ctcaaagagt tcgtcgaagt cgacaaggac ggcctggtaa
     1561 cgatccaccg cgccgtccag gtagtcggtc tcggcggcga tccggagagg gaacgttacc
     1621 gcgacggaac ctacgagtac tacgtgaccg agaaggtccg cgacaacgat ccgaaagcag
     1681 tcgggccgtt cattttcgca agcatggaga tggagaggta gtcttctatc cgggtgatcc
     1741 cgtgaatccc aatcgcgatg tctccaccca aggggcagat caagcgcgtt tcaccgaatg
     1801 cttcttcttc gcgcgctgtc gcacggcggc ccgcatctca gtgatcgaga cggttttgcg
     1861 tctggcaagc cgctgaagca tgccgaagcc acgatccagg ggcggcggtt gttggcgttc
     1921 gagcgtcagc aacccatcgc ggccaatcgc tatatcgaac cggtcaccct gctttagcgc
     1981 aagccgatca cggatgtcct tcgggagcgt gatctgtcct ttggacgtca aggtcgtgga
     2041 actcaaaacc gcctccttac ccaacgcaga gtatcgctga tctggtaagg gtgtcccctt
     2101 tcgaaagcaa cgactgatct gacggggaga cgatggggag atgtcgaaga gcttctctct
     2161 ccccgcacta tgcgcctctc tttgagtcct tccgtccaga aagcaggcgc taccgtgtgg
     2221 gttcagcgct ggccaggagt cccaccctct tccaaagcgc cgcgagatct agcgtcgtgt
     2281 catcgacgag agcatttgac aacgattcga gccacttcct ccgcgcatcg gcaccatccg
     2341 gaaccgaaag aactcgattg gtctgcgctc gcaccgggta tccgcctacc tgttcctggc
     2401 tgtcgatgat ccttccagtt ccgtcgatca ccgatcgcac gatgcgcgcg tcgatcggat
     2461 cgcggtgggc ggggcgggcg ccggctgagc ggagaacgtg agcggcagca tcccagggat
     2521 gaatgtcggg tagatcgcga ggcgaaggaa gtggaacgac accatcgtcc gtcattggaa
     2581 gaaccgcacc attcgcggcg actgcaacgt tgtcgcgcag gaagacccgt ccgcccggat
     2641 cgacactctt caccaagact tgcgagtgcg tgtctgcccc agcgatcgca acgtttccga
     2701 cgagcaccgt ctcggaaggt tgcagttcct tacgatttcc atgctttccg acgccgacgc
     2761 acgctgagcc ccagttgtac atgacgttcc cggcaacgat ggacgtcgtg ccacctttga
     2821 ggcgagggtt gcgctcgcga ttgtgcgcat agagacagcc gacgatcacc acattgcgga
     2881 ctccatcgtg gaccagtgtc cccttcgagt gctcgccctt tggatgcgtc gcgtgcgaca
     2941 gaccttcggc aatgaggcaa aaccgcagcg tgatgtcgtg ggatgtaacg tcgggatccc
     3001 tggtcgagtc catatctgct ggtccggatg ccgatagatt ctcgtcgatg gcccaggttg
     3061 ccgagcagtg atcgaagacg acgtggtgaa ccgggccggc gttcccgcgg tgcgcgccga
     3121 gggcgtcggg agcccattcg ctctgagcca aaccacgtac cgatcctgct cctccgtcgc
     3181 ctgggcggac agcgatgtgt tgaatgacga cgtcatgagt ctcgaccgtg aggctcccac
     3241 gaatcagcgt gatgcctgga tcgggggcgg tctggccggc gacagtaacc cagggatgcc
     3301 tgacatcgag ggaccggccc tgcaaatcga tcactcctcc gacttcgaag acgacgaggc
     3361 gcggacgcgt gtcctccaag gcgcggcgaa gagagcctgg accgtcagcg gcgagcgtgg
     3421 tgacccgaag taccccatcg agccccccga tagtcgtttc ggctagacct attggtaata
     3481 ccaatgatga cgcgatcact gcttgctgtt tgtcatcgtg tccaccctgt cgcgttggcg
     3541 ctggtatcac tgcgcaagcg aaaacgatag ccgcgagagc aacggaggag atcacatggt
     3601 tgagtgattt tgtcatattg gtaagacaac acttgactag catgcgttag tggtcatata
     3661 acatctccgt tctcaaacca agcaaatcct gcgcgaaaac gaggaggtcg cccccatgaa
     3721 gcaacgaatc agccttgcgg tgctcatcgt cctggtcctc attctcatca gcgctcctct
     3781 cgcggcgcag gaatcccgag gggcgatcga cggcatcgtg aaagacacat ccggggcact
     3841 ccttcccggc gtgacgctca cggtcaagaa cgtcgaaacg aacgtcaccg ccatgtcggt
     3901 gtcggactcc aaaggcgcgt accacgtctc tcacctcaat cctggaacct attccgtgga
     3961 agcgggactc gacgggttca agaccgaggt ccggaaaggc atcgcggtcc atgtcggcgc
     4021 tacggttacc gtcgacttca agatggagca gggcgccctc tcggaagtca tccaggtgac
     4081 tgcgagcacg ccgctgctcg acacctcgtc tcccgccaca gggcaggtga tcgacagcgc
     4141 gcagatccag cggcttcctc tcggagacgg tactgcctac atgctgtcgc gcctcgcgcc
     4201 cggcctggtt gacaactcag acctccactt tgaccggccg atggacaacg ccggactggc
     4261 cggaatcgtc gccaacggct cgcagggcgg caacgacttc acccttgatg gtgcaccgaa
     4321 caaggtgtct cccaatacga gcagccccgg caacaatagc ggtgtggtcg gcttctcgcc
     4381 tccctcggac gccatcgccg agttcaaggt gcagacgaat gcattcgacg ccgaatcggg
     4441 tcactccgcc ggcgcgaccg tgaacctggc gttgaagagt ggaaccaatg gcgtgaaggc
     4501 gaacgtttcg tacttcaatc gcgacagcag caggacggcg acgccgctcc tcacggagcg
     4561 tgccgacgcc ccaaaaccgc cccggcaata caaccgcgag acgtcgacaa tcgccggtcc
     4621 catcgtccgg gatcgcacct tcttcatggt ttcctacgag catctccgtg acgtgaacgc
     4681 cgagcccgcg acctataccg tgccgaccgt caaaatgcgg cagggtgatt tctcagagtt
     4741 ctcgggtctg atctacgatc cactgacggc caccgggagc aacctcactc gcaagccgtt
     4801 ctcgaacaac cagattccaa tcgagcggat caacgccgta gccaaggcgt acgccgctct
     4861 ttatccgctc cccaacaaac ccggcacgag cggcaactac ttcaccaacc agttgcggcc
     4921 ctacgactac aacgccatcc tcgggcgtgt cgatcacaac atcaacgact ccaacaagct
     4981 cttcatcgac gggtactaca acaagcggca ggaggaccgg tataactggg ccctcggagc
     5041 cgcaaatgcg aataacggca ccatcaacgg cttcgacgtc actcacggtt tcgactatcg
     5101 aagcaacacc ggcacgacgc tcggttacac caacgtccga tcgagcaacc tcgtgttcga
     5161 cgtccgcacc gcctggtcgc agttcggcga gtggcgacag gcgggagacc agttcgatcc
     5221 ggcaagcatg ggtttctccc cgcaggccgt ggcgttgatg aatggataca agtacctgcc
     5281 gttcatcacc ttcggcggat tcagcacgac aaactccggc tcgacgatcg ccacgctcgg
     5341 ttcgatgcga tcggactggg gaaccggttt caaccggccc tttaccaaca tctcgttcgt
     5401 gcccagtgcc gactggcaga tttacggcca ttccatccgc accggttacg agcttcgtga
     5461 tcaacactgg aagatcgaag ttcccgcgta tggcgcggga cgctacaact tcaacggtgc
     5521 gtacacccgg ttgaacaact ccgcgccgct gaacgaccag gcgcaggagt gggcacagtt
     5581 tcttctcggg ctccccacca caggaaccgg tgcggtcgga aattcaggcg ccgtttcgag
     5641 ccagttcgag atcgcagcca aagcgaatta cagtcaggtc tcgcacgcct tcttcgtgca
     5701 ggacgactgg cagctcaact cgaatctgac cgtgaacctc ggcgtacggt tcgagttgga
     5761 ccaggccatg accgaggcgc acaaccaagc catttccggc tttgatactt cgatcagcag
     5821 tccgatcgaa gccgcggcgc tggcgaacta cgccaagaac ccgatcgcgc agattgccgt
     5881 cggtgacttc catgttcggg gaggtttgca gttcaccgac ggagggatct acaacgacct
     5941 tcacaaggtg atgccccgcg ccgccttcgc ctaccagatc ggagataaaa cggtgctccg
     6001 cgccggagcg ggcctcttca gttacgacta ttacttcgat gccggcaacc aaacgggctt
     6061 ctcgcagccg acggcaatcg tcaccacgga caacaatggc gcgaccttcc tcgcgaatct
     6121 gagcaacccg attccgactg ggggcctgat tcagcctact ggtgcctcgc tcggtgctgc
     6181 caccggtctc ggattggcga ttggttcggt cacgccctcg cagcgcgaaa gcccgtacta
     6241 ccggcgttgg cagatcggcg gccagcatga cctcggtgcc ggctggaaga tggaagtcaa
     6301 ctatctcgac tcgcgcggta cgcatctgcc ggtgcagcgc gaattgaacg gtattccgta
     6361 ccaatacctc tccacatcca acattcggga caccgcacaa gagagcttcc tgagcgcctc
     6421 ggtcgcgaat cccttcgccg gcatgctgcc cggaacgacc ctcaacggcg caacgaccac
     6481 gcgcggccag ctcctgaagc cttttcccga gttcctgagc atcgttaccg aggagtaccg
     6541 cggttccgac gcctatcgcg ccgcctcggt gtcgctggag aagcggttca atcagggcgc
     6601 ctccctgaca gcctcctata cccgttcacg gctgacggac aagctcaaca tgctgaaccc
     6661 gtcgaacggc attctcgaga accgcgtttc gcccaatgac cgtccgaatc gcgccacatt
     6721 cggagggacc gtcgatcttc cgtttggtgc cggcaagaag tggggctcca actggagtgg
     6781 attcacgcag gcgctcatcg gcggctggtc cgtcagcgcg acctatcaat atcagagcgg
     6841 tttccccctg agctggggca acgtctacta cgatccgtcc cgcaacccga acgatctcca
     6901 ttcccacatc ggcaaacacg tctgccccgg taacccgaac ggagtcgccg gtctcgattg
     6961 tcccgcctgg gatacctcgg gcttcttcat cgcgggcggc accggccgca ccgattcacg
     7021 catccagctc gggaacaacg ttcgcacgtt cccgtcgaca ctgtccaatg tccgctccga
     7081 tcctcttcag ctgttggacg tcggcatcta caagaacttc ggtcttcccc gcggcatgac
     7141 cttccagctc aggatcgaat cgatcaacgc gctgaactac accgtgctct tcaactccaa
     7201 catggacccg cgcaacgcac agttcggact cgtaaaccag gaccgcaaca atccgcgcga
     7261 catccaactc ggcgggcggc tatccttcta aggtgaggtt gcc
//
LOCUS       DATLVW010000045         5085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_126832, whole genome shotgun sequence.
ACCESSION   DATLVW010000045 DATLVW010000000
VERSION     DATLVW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126832"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..1185)
                     /locus_tag="VNN25_01595"
     CDS             complement(517..1185)
                     /locus_tag="VNN25_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90245.1"
                     /translation="MGLWRAPRLGAGNESLSDSRLPTPGFARNTVVFLDNDEGGVTGF
                     IEEFWGVRVRAKNIKEARKQLKDAAQTFIDENREDILRQMDSDGPVTREKMLLEIWRR
                     PQSGVDFNREIEDEEKHMLARKSPSIVVFAILVTAMLCVASCTTTRSDENVRLKTARA
                     IGGVTSEQVTISNLHSWMKTRPSGFALAVSPVSENWEAATPKGHYICESDDWLKRGAN
                     CVKR"
     gene            1974..2693
                     /locus_tag="VNN25_01600"
     CDS             1974..2693
                     /locus_tag="VNN25_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90246.1"
                     /translation="MTRWFPASAFAFLLLSACVTSQPIHQVGAPDPVTDPRFTGDARP
                     MSDPRVSGDLNPVADATNVGAVDEAARESRERAEAGARTGRRVGVVAGTLAAVLGGPS
                     RDSVEGMVGRYVITRDAATLAGAIIGGVKGGKEGAKRGSELDLQFAELKKIDGLDVTR
                     PTPDRIDVFMETVPNHETLAAISAVFAGHEQRWVDIEAADPIPLDLRDSLIDLGVPES
                     SISVQRNDGLRGVILRIRYQS"
     gene            2765..>5085
                     /locus_tag="VNN25_01605"
     CDS             2765..>5085
                     /locus_tag="VNN25_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXH90247.1"
                     /translation="MATDMRTDAGAVLSTSELNLIHAWWRAANYLSVGQIYLLANPLL
                     REPLLLEHTKPRLLGHWGTTPGLNFIYVHLNRVIKARDLSVIYIAGPGHGGPGLVANT
                     YLEGTYSEIYSNIGQDADGLRKLFKQFSFPGGIPSHVAPETPGSIHEGGELGYALVHA
                     YGAVFDNPDLIAACVVGDGEAETGPLAASWHSNKFLNPARDGAVLPILHLNGYKIANP
                     TVLARISNDELRDLFRGYGYEPHLVEGDDPATMHQLMASAMDAVLDEIRAIQTAARTN
                     GFSERPIWPMIILRSPKGWTGPKEVDGKPAENSWRSHQVPFSELAGNPAHLKLLEQWM
                     KSYKAEELFGEEGRLRPEIAELAPRGERRMSANPHANGGVLLRDLRMPDFRDYATDVD
                     VPGTKLAEATRIAGGFLRDIIRKNPDNFRLVGPDETASNRLNAVFETTDRVFTGTILP
                     TDDHITHDGRVMEVLSEHLCQGWLEGYLLTGRHGLFSCYEAFIHIIDSMFNQHAKWLK
                     TTRHIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVTNKKAEVVRVYLPPDAN
                     TLLSVMDHCLRSRNYVNVMIAGKQPALQYLDMDAAIKHCSAGVGIWEWASNDENAEPD
                     VVMACAGDVPTLEILAAVDIVRQRIPDLKVRVVNIVDLMALQPQSEHPHGLSDADFDS
                     IFTTNRPVIFAFHGYPWLIHRLTYRRRNHDNLHVRGYKEEGTTTTPFDMTVLNNLDRF
                     HLVIDVIDRVPGLNDRCAHVKQDMRDMLVEHHE"
BASE COUNT          943 a         1612 c         1523 g         1007 t
ORIGIN      
        1 cgcccacact cctctgttcg gagtgcggtc gtctcgaccg cgggcactcg ggcgtcctcg
       61 cccgagtgct gtcagggcgg aggtcgaaat ccgttaacgt cctttcttga gcaataccct
      121 catgccattt agcccttgaa atcctcaaga ggcgcgtcga agtcgggagc catgtagtgg
      181 gagtggggtg tgggggcttt gcacacaaac tccggcctgc acataaagtc gggtggctgc
      241 acataaagtc gctgaggatg gctcgggtac cttcgctcaa gccagctcac gcgtctgttc
      301 gccgaatggc gtcaagctgc gagtagtagt ccgtgtggcc gcaagcgttg caggctatcg
      361 tctctgcggt tcacccgcac gaaccgcgac gcgaagacct atgggttcgc gatgcggcaa
      421 cgcagcggtt cgtgtcgggt gcaaccgctt gttgggcggc gccgagcctc gaaagctaag
      481 gcacagagtt gcgcgagatg cccggtcggg cagacgctat cgcttgacgc agttggcacc
      541 gcgcttaagc cagtcgtctg actcgcatat gtagtgtcct ttgggcgttg cagcttccca
      601 gttctcggac accggcgata cggccagggc gaatccagac ggcctggtct tcatccagct
      661 atgaaggttg ctgattgtga cctgctctga ggtgacgccg ccaatggctc gagccgtttt
      721 aagacgaaca ttctcgtccg agcgagtggt ggtgcatgaa gcaacacaaa gcatggcagt
      781 tacgagtatc gcgaacacaa ctatcgacgg ggactttcga gcaagcatgt gtttctcctc
      841 gtcttcgatt tcacgattga agtctactcc tgactgtgga cggcgccaaa tctccagcag
      901 catcttctcg cgcgtcaccg gaccatccga gtccatctgc cggaggatgt cctcgcggtt
      961 ctcatcgatg aacgtctgcg ccgcgtcctt gagttgcttg cgggcttcct tgatgttctt
     1021 cgcgcggacg cgaacgcccc aaaactcctc gatgaagccg gtgacccctc cttcgtcatt
     1081 gtcgagaaag acgaccgtat tgcgtgcaaa gccgggtgtg gggagtcggg agtcggagag
     1141 cgattcattt ccagctccca gtcgcggcgc acgccagaga cccatggtga tctggcttgt
     1201 ttctccgact cccgaccccc gacgcgctac acccgcacgc caagcgtgct gttcggcttt
     1261 tcggccgaaa aacgcgtttc tctgacgtgc tgttacgttt ttccgaggga aaacgcgttc
     1321 atccgatgtg ctgtcgcgct tgttcgccga aaaacgtgtt tttcctgcgt gctatcgcgt
     1381 ttttccggcg agtcgtcacg tttttccggc gtgctgtcgc gtttctccgc cgaaaaacgc
     1441 gtttttccgg cgtgctgtcg cgtttttccg agaaaaaacg cgttcacccg gcgtgctgtc
     1501 gcgtttttcc tgcgaaaaac gcgttcatcc ggtgtgctgt cgcgtttttc cgcgaaaaaa
     1561 cgcgttcatc cggcgtgctg tcacgttttt cagccgaaaa acgcgtttat ccgcgctcgt
     1621 tcaggacaac caggtggctt gtcttgtcct cgaaaagaga cgcgttggcc tccgcacacg
     1681 ccgctcctcc cgttcgccga ggtcttccgg caaacgggga gcggcggagc aacattcatc
     1741 tccccgctgc atcattcatt acacgctcga gcccgaggcg cgccagcgta acgataaccg
     1801 tcgaccatgc atgagcttgc aagcgatgtg atcgcacgac acccgcagca ccgtcgtcgg
     1861 cgctccccct ccagacgcgt ggcgagcgaa tcgatcctcc gctggcaggg gcattgcgaa
     1921 ccaacggctg tcgccatcgc ccgctgctac agactttgac tggagatcgc atcatgactc
     1981 gctggttccc ggcctccgca ttcgccttcc ttcttttgtc tgcctgcgtg acgtcgcagc
     2041 ccatccacca agtgggcgct cctgatccgg taaccgatcc tcgcttcacc ggtgacgcca
     2101 gaccgatgtc cgatccacgc gtttccggcg acctcaatcc tgtcgcggac gcgactaacg
     2161 tcggcgcggt cgatgaagcg gcgcgggaat cgcgggaacg ggcggaggcc ggtgcgcgca
     2221 ccggacgccg cgtcggcgtt gtggccggaa ctctcgctgc ggtgttgggt ggaccgagcc
     2281 gcgattctgt agaaggtatg gttggccgct acgtcatcac gcgggacgca gccaccctgg
     2341 ccggtgcgat catcggcggc gtgaagggcg ggaaggaagg ggcgaagcgc ggatccgagc
     2401 tcgatctgca gtttgccgag ttgaagaaga tcgacggcct cgatgtgact cgcccgactc
     2461 ctgatcgcat cgacgtcttc atggagaccg tgccgaatca cgagacgctc gccgcgatct
     2521 ctgcggtctt cgccggacac gagcagcggt gggtggacat cgaagcagcc gaccccatcc
     2581 cccttgatct tcgcgactcc ctcatcgacc tcggcgtccc cgagtcgagc atcagcgtgc
     2641 agcgcaacga cggactccga ggcgtcattc ttcggattcg ctatcagagt tgacgtcgcg
     2701 attcgtttcg ccgaaacgat tggctcatgg aatccgtaac tacgcggtgg catgatgcct
     2761 gcgcatggct accgatatgc gaaccgacgc cggcgcagtt ctctcaacga gtgagttgaa
     2821 cctcattcac gcctggtggc gtgcggccaa ctacctctcc gtcggccaga tctatctgct
     2881 cgccaacccg ttgctgcgcg agccgctgct gctcgagcat acgaagccgc ggcttcttgg
     2941 acactggggg acgacgccgg ggctcaactt catctacgtg catctcaacc gcgtcatcaa
     3001 ggcgcgcgac ctcagcgtga tctacatcgc cgggccgggt cacggcgggc cggggctggt
     3061 tgcgaatacg tatctcgaag ggacgtacag cgagatctat tcgaacatag gtcaggatgc
     3121 tgatgggctg cggaagctct tcaagcagtt ctcgtttccc ggcgggatcc ccagccatgt
     3181 cgcgccggag acgcccgggt cgattcatga aggcggcgag cttggctacg cgctcgtgca
     3241 tgcgtacggc gcggtcttcg acaatcccga tctcatcgcc gcatgcgtcg tcggcgacgg
     3301 cgaggcagag acggggccgc tggcggcgag ctggcactcg aataagttcc tcaatcctgc
     3361 tcgcgacggc gccgtgctgc ccatcctgca tctcaacgga tacaagatcg ccaatcccac
     3421 agttctggcg cgcatcagca acgatgagct gcgcgatctc ttccgcggct acggctacga
     3481 gccgcatctc gtcgaaggcg acgacccggc cacgatgcat cagttgatgg cgtcggcgat
     3541 ggacgcggtc ctcgacgaga tccgcgctat ccagaccgcc gcgcgaacga acggattctc
     3601 cgagcggccg atctggccga tgatcatcct gcgcagtccg aaagggtgga ccggaccgaa
     3661 agaggtcgac ggcaaaccgg ccgagaactc ctggcgatcg catcaggtcc cgttttccga
     3721 gctggccggc aatcccgcgc acctcaagct gctcgaacag tggatgaaga gctacaaagc
     3781 cgaggagctg tttggtgaag aggggagatt gaggccggag atcgcggagc tcgcgccgcg
     3841 cggcgagcgg cgcatgagcg cgaatccgca cgcgaacggc ggagtgctcc ttcgcgatct
     3901 gcgcatgccc gactttcgtg actacgcaac ggacgtcgat gttccgggca cgaagttggc
     3961 ggaggccacg cgcatcgccg gagggtttct gcgcgacatc atccgcaaga accccgacaa
     4021 cttccggctc gtcggtcccg atgaaaccgc gtccaatcgc ctcaacgccg tcttcgagac
     4081 gaccgaccgc gtcttcacgg gaacgatcct tccgacggac gatcacatca cgcacgacgg
     4141 ccgcgtcatg gaagtgctga gcgagcatct ttgccaggga tggctggaag gctacttgtt
     4201 gaccggccgg cacgggctct tctcctgcta cgaagcgttc attcacatca tcgactcgat
     4261 gttcaaccag cacgcgaagt ggctgaagac cacgcgccac attccctggc ggcggccgat
     4321 cgcctcgctc aactacctgc tgacgtcgca cgtgtggcgg caggatcaca acggcttcag
     4381 ccaccaggat cccggcttca tcgatcacgt cacgaacaag aaggccgagg tcgtgcgcgt
     4441 gtacctgcct cccgacgcga atacgttgct ctccgtgatg gatcactgcc tgcgcagccg
     4501 caactacgtg aacgtgatga tcgccgggaa gcagccggcg ttgcagtacc tcgacatgga
     4561 cgcggccatc aaacattgct cggccggcgt tggtatctgg gagtgggcaa gcaacgacga
     4621 gaacgccgag ccggacgtgg tgatggcttg cgcgggcgac gttcccacgc tcgagattct
     4681 cgctgccgtc gacatcgtgc ggcagcgcat cccggacctg aaggtccgcg tcgtcaacat
     4741 cgtcgacctg atggcgctgc agccgcagtc ggagcatccg cacggactct ccgacgccga
     4801 tttcgattcg attttcacga ccaaccgccc cgtcatcttc gccttccacg gctatccgtg
     4861 gctcattcat cgcctgacct atcgccgcag gaaccacgac aaccttcacg ttcgcggata
     4921 caaagaggaa gggacgacga ccacgccctt cgacatgacg gtgctcaaca atctcgaccg
     4981 cttccacctc gtgatcgacg tcatcgaccg cgtgccgggg ctcaacgacc gctgcgcgca
     5041 cgtgaagcag gacatgcgcg acatgctcgt cgaacatcac gagta
//
LOCUS       DATLVW010000046         4304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_208879, whole genome shotgun sequence.
ACCESSION   DATLVW010000046 DATLVW010000000
VERSION     DATLVW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208879"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VNN25_01610"
     CDS             complement(<1..570)
                     /locus_tag="VNN25_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXH90248.1"
                     /translation="MESFNSLFQSTKPQAINDFNAIKISLASPEKIRSWSYGEVTKPE
                     TINYRTFKPERDGLFCAKIFGPITDWECLCGKYKRMKHRGVICDKCGVEVTKSKVRRE
                     RMGHIELASPVSHVWFFKGLPSRIGHLLDISLRDLERILYFESYVTIDPGDLSNVLDE
                     KELLTEERYRELRTAHGDVFVAKMGAEAIK"
     gene            complement(589..780)
                     /locus_tag="VNN25_01615"
     CDS             complement(589..780)
                     /locus_tag="VNN25_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90249.1"
                     /translation="MAKKVTAVASMPDASCPACQGMGAVSMTAHFPSGDQEWEAPCWM
                     CYGNTVALSLPTPRSKDNA"
     gene            complement(784..>4304)
                     /gene="rpoB"
                     /locus_tag="VNN25_01620"
     CDS             complement(784..>4304)
                     /gene="rpoB"
                     /locus_tag="VNN25_01620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXH90250.1"
                     /translation="YVRIDRKRKFLGSVFLRALGLTTDEEILRQFYTPIKAKLTGDQV
                     TLSFDRRVLDQEEAKERHSIRGRRTVRTVFAGVKLDQKMADALTKHNEASAKVDFAAM
                     DKAVFLSDVVDVNSGEVLFEASENLPADWADVLRAHDVESVDVIFPEWDLVSDILLNT
                     VRKDTAKSFEAAIIEIYRRMRPGDPPTLESAKALFEGMFFDARKYDFSRVGRFKFNIK
                     LDLDSPVTQKTMSAEDFFVVINYLLRLRKDVGRVDDIDNLGNRRVRAVGELLENQFRI
                     GLVRMERAIKEKMSVHQDIDSAMPHDLINSKPVIAAIKEFFGSSQLSQFMDQTNPLSE
                     VTHKRRLSALGPGGLSRERAGFEVRDVHPTHYGRICPIETPEGPNIGLISSLACYARI
                     NEYGFIESPYKKVENGRVLDHYRIVKVGDSSFTLSQIVEKRDLQKENNRIGKENAGAK
                     TRKGQLALAEAEPYAFYLSAWDEERYTIAQANVVMDEEGNLVHDRVIARQSGDFVSIE
                     KEKVDFIDVSPKQLVSVAASLVPFLENDDANRALMGANMQRQSVPLLRTDSPLVGTGM
                     ENIVARDSGAVVLCKRAGIVDLVDSNRIIVRVEAEDVETGETKEFGADIYQLIKFKRS
                     NQNTCITQKPNVREGQRVRKGQVLADGPCTDAGELALGRNILVAFMPWRGNNFEDAIL
                     VSEKLAKEDYYTSIHIEEFEIEARDTKLGPEEITRDIPNVSEGALRDLDESGIVRIGA
                     TVKQGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRDASLKTPPGIEGTVVDVKI
                     FSRKGVEKDLRAKSIEEGEIERMNRNIQDEIRIITDARNKRIGDVLSDKKLQRDVVDF
                     RSGETLVGKGQTATKETVNRLSRRELLALPVDEETRETIRMFVERAENRIRVLEQKAE
                     ERREDLQKGDELPPGVIKMIKVYVAMKRKLSVGDKMAGRHGNKGVISRILPEEDMPYL
                     PDGTPVEIVLNPLGVPSRMNVGQILETHLGWAARVLGLYFSTPVFDGAIESEIKAQLK
                     AANLPDDGKTPLFDGMTGEQFEQRVCVGYIYMLKLSHLVDDKIHARSIGPYSLITQQP
                     LGGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTSKSDDVEGRSKIYEAIVKGEVPDD
                     PGLPESFNVLVRELQSLCLDVELLKE"
BASE COUNT          752 a         1245 c         1389 g          918 t
ORIGIN      
        1 cttgatcgcc tcggcgccca tcttggcgac gaagacgtca ccgtgcgcgg tgcgaagctc
       61 gcggtagcgc tcttcggtga gcagctcttt ctcgtcgagg acgttcgaca ggtcgcccgg
      121 atcgatcgtc acatacgact cgaaatagag gatgcgctcg aggtcgcgca gcgagatgtc
      181 gaggaggtga ccgatgcgtg acggcaggcc cttgaagaac cagacgtgcg aaacgggcga
      241 ggccagctcg atgtgtccca tccgctcgcg gcgcaccttc gacttcgtca cttcgacgcc
      301 gcacttgtcg cagatgactc cgcgatgctt catgcgtttg tacttgccgc agaggcattc
      361 ccaatcggtg atcgggccga agatcttggc gcagaagagg ccgtcgcgct ccggcttgaa
      421 ggtgcggtag ttgatcgtct ccggcttggt tacttcgccg tacgaccacg accggatctt
      481 ctccggagac gcgaggctga tcttgattgc gttgaaatcg ttgatcgcct gcggcttggt
      541 ggactgaaac aatgagttga agctttccaa attgcgcctc cctatgtgtc aggcgttatc
      601 ttttgaacgc ggtgtcggca acgaaagcgc cacggtgttg ccgtagcaca tccagcaggg
      661 ggcttcccat tcctggtcgc ccgaggggaa atgtgcggtc atgctgaccg ctcccatacc
      721 ctgacaggca gggcaggaag cgtcgggcat actggcaacg gctgtgactt ttttcgccat
      781 tcgctattcc ttcaggagct cgacgtcgag acagagcgac tgcagttcgc gaacgaggac
      841 gttgaatgac tcgggcagac ccggatcatc cggaacctcg cccttgacga ttgcctcgta
      901 aatcttggag cggccctcga cgtcgtcgga cttcgaggtg agcagctcct gcaaggtgta
      961 ggcggcgccg tacgcttcga gagcccagac ttccatttct ccgaagcgct gaccgccaaa
     1021 ctgagccttg ccaccaagcg gctgctgtgt gatgagtgaa tacggtccga tcgaacgcgc
     1081 gtgaatcttg tcgtcgacca gatgcgacag tttcaacatg tagatgtagc cgacgcagac
     1141 gcgctgctcg aactgctcgc cggtcatgcc gtcgaagagc ggggtcttgc cgtcgtcggg
     1201 caggttcgcg gccttgagct gcgctttgat ctcggactcg atggcgccgt cgaacacggg
     1261 cgtcgagaag tagagcccga gaacgcgggc ggcccagccg aggtgcgtct cgaggatctg
     1321 accgacgttc atgcgtgacg gaacgccgag cggattgaga acgatctcga ccggcgtgcc
     1381 atccggcagg tacggcatgt cctcttcggg gaggatgcgg ctgatgacgc ccttgttacc
     1441 gtggcgtccg gccatcttgt cgccgacact cagcttgcgc ttcatggcga cgtagacctt
     1501 gatcatcttg atgacacccg gcgggagctc atcgcccttc tggaggtctt cgcgacgctc
     1561 ttcggccttc tgctccagca cgcggatgcg attttcggca cgctcgacga acatgcggat
     1621 tgtctcgcgc gtctcttcat cgaccggcag cgccagcagc tcgcggcgcg agaggcggtt
     1681 gaccgtctcc ttggtcgcgg tctgaccctt gccgacgagg gtctcgcccg agcggaagtc
     1741 gaccacgtcg cgctgcagct tcttgtcgct gaggacgtca ccgatgcgct tgttgcgggc
     1801 gtcggtgatg atgcggatct cgtcctggat gttgcggttc atccgctcga tctcgccctc
     1861 ttcgatcgac ttggcgcgaa ggtctttctc gacgcccttg cgcgagaaga tcttcacgtc
     1921 gacgaccgtg ccttcgattc cgggaggcgt tttcagcgag gcatcgcgaa catcgccggc
     1981 cttctcaccg aagatggcgc ggagcagctt ctcttcagga gtgagctgcg tctcgccctt
     2041 cggcgtgact ttgccgacga ggatgtcgcc ctgttttacg gtagctccga tgcggacgat
     2101 gcccgactcg tcgaggtcac ggagcgcgcc ttccgacacg ttcgggatgt cgcgcgtgat
     2161 ctcttccggt ccgagcttcg tgtcgcgggc ttcgatctcg aactcttcga tgtggatcga
     2221 ggtgtagtaa tcctctttgg ccagcttctc ggagacgagg atggcgtctt cgaagttgtt
     2281 gccgcgccac ggcatgaagg caaccaggat gttgcgtccc aaagccagct caccggcatc
     2341 ggtgcagggc ccgtcggcaa gcacctggcc cttgcggacc ctttgccctt ctcgtacgtt
     2401 tggcttctgc gtgatgcagg tgttctggtt ggagcgtttg aacttgatga gctgatagat
     2461 gtcggcgccg aactctttgg tctcgccggt ctcgacgtct tcggcttcga cgcgaacgat
     2521 gatgcggttc gagtcgacga ggtcgacgat gccggcgcgc ttgcagagaa cgacggcgcc
     2581 ggagtcacga gccacgatgt tctccatacc ggtgccgacg agcggcgagt cggtgcggag
     2641 gagcggaacg gactggcgct gcatgttggc gcccatgagc gcgcggttcg cgtcgtcgtt
     2701 ctcaaggaag ggaacgagcg atgcggccac ggaaacgagc tgtttcggcg agacgtcgat
     2761 gaagtcgact ttttcttttt caatagatac gaagtcgccg gactgccgtg cgatgacgcg
     2821 atcgtgaacg agattgcctt cctcgtccat caccacgttg gcctgcgcga tcgtgtagcg
     2881 ctcttcatcc catgcggaga gatagaacgc gtacggctcg gcttcggcaa gcgcgagctg
     2941 gcccttgcgg gtcttcgcgc cggcgttctc cttgccgatg cggttgtttt ccttctgcag
     3001 gtcgcgcttc tcgacgatct ggctgagcgt aaagctcgag tcgccgacct tgacgatgcg
     3061 gtagtgatcg agcacgcggc cgttttcgac cttcttgtac gggctctcga tgaaaccgta
     3121 ctcgttgatg cgggcgtagc acgccagcga cgagatcaga ccgatgttcg gaccttcagg
     3181 cgtctcgatc gggcagatac ggccgtagtg cgtcggatga acgtcgcgca cttcgaatcc
     3241 ggcgcgctcg cgggagagac cacccggtcc caaggctgac agacgccgtt tgtgcgtgac
     3301 ttcggagagc ggattcgtct gatccatgaa ctgcgagagc tgcgacgaac cgaagaactc
     3361 cttgatggca gcgatgaccg gcttggagtt gatgagatcg tggggcatcg ccgaatcgat
     3421 gtcctggtgg acggacatct tttccttgat cgcgcgctcc atgcgcacaa ggccgatgcg
     3481 gaattggttc tcgagcagct cgccgactgc gcggacacgg cggttgccga ggttatcgat
     3541 gtcgtcgacg cggccgacgt ccttgcggag gcggagcagg tagttgatga cgacgaagaa
     3601 gtcttcggcc gacatcgtct tctgcgtaac gggcgaatcg agatcgagct tgatgttgaa
     3661 cttgaaacgg ccgacgcgcg agaagtcgta tttgcgggcg tcgaagaaca tgccttcgaa
     3721 cagtgccttg gccgactcaa gggtcggagg atcgcccggg cgcatgcggc ggtaaatctc
     3781 gatgatggcc gcttcgaacg acttcgcggt gtctttgcga acggtgttga gcaggatgtc
     3841 ggagacgaga tcccactccg ggaagatgac atcgacggac tcgacgtcgt gcgcgcgaag
     3901 aacgtcagcc caatcggcag gcaggttctc gctcgcttcg aagagaacct cgccgctgtt
     3961 gacgtcgacg acgtcggaga ggaagacggc cttatccatc gccgcgaagt cgaccttggc
     4021 ggacgcttcg ttgtgcttgg tcagagcatc ggccatcttc tggtcgagct tcacgcctgc
     4081 gaacacggtg cggaccgtgc ggcggccgcg gatcgagtga cgctctttcg cctcttcctg
     4141 atcgaggacg cggcggtcga acgacagcgt gacctggtcg ccggtgagct tcgccttgat
     4201 cggcgtgtag aactggcgaa ggatctcttc gtcggtcgtc aatccgaggg cgcgaaggaa
     4261 gacggaaccg aggaacttgc gcttgcggtc gatgcggacg taga
//
LOCUS       DATLVW010000047         6763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_498187, whole genome shotgun sequence.
ACCESSION   DATLVW010000047 DATLVW010000000
VERSION     DATLVW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498187"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1698)
                     /locus_tag="VNN25_01625"
     CDS             complement(64..1698)
                     /locus_tag="VNN25_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90251.1"
                     /translation="MPANSRFKIAYALLIVCAAARLLLQMAALPPYAGLDEIYHVARL
                     AFVRAEHRNPTTTEPSIPPYLVASIAAAQGDALRRSDSQSDKRVQSDWRVRPTSSAIA
                     MPAMGESGDRWPSIVAERGGHILDDRPLAAADVKPYVVRNYEAQQTSIYYSLTACLVP
                     LRSAIFELRMWRLLSLLFALITVIATAEIGRRWIGPIGILGGAILVSLPTWETLILRA
                     SNDAFACMLVAVAIAISVAAPRRPLIIAMEALTWGLALNAKMYTWPLLIVLPLLWRRE
                     KAGKARIAAVSAVSLIAIVLTFAELSARTSTEVVQYPGGSPLGVVALAHQAGPVHEAG
                     ANVSEIVRVTIASAAWTSGQHNDALRPAAIALYLGPVIIAMIVALRRENVAPALLTLG
                     AFALAQTYNVIVCVLAQRAGSQVPIGGKEGWYWFVLVPIVVPALLLPALSRWRLAAWW
                     IVAWDVVITEVALFQDFAGASSPAHGSMLFRWGPLHLPFTAHLGGIGVGPFAAWTTLI
                     RVAHLGAFFGLESILQESTKTKLRLNDRNTDIAHPD"
     gene            1827..3080
                     /locus_tag="VNN25_01630"
     CDS             1827..3080
                     /locus_tag="VNN25_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH90252.1"
                     /translation="MATHDPYAALRVRNFLWLILGYATSTVAREAQIVVVGWQVFQVT
                     RDPLALGMIGLAEALPFIAVALYAGHVADRLPRRPIAIVGTFGMLLSAIALLLFTTSG
                     MIARGHVWPIYLVIFLSGILRSFIRPAFTALSAEVVTRDLFANAVAWRSSTWQFAAVL
                     GPAIGGLIYGFAGPAAAYAVVIVLMTLALGALWFIAHDVRPQQAEEIPIGESLRIGIR
                     FLLGQRILLAAMTLDLFSVLFGGAVALLPIFAHMLGAGPQGLGVLRAAPAAGSLVTGI
                     IVAHRRPMRRTGAALFAAVAIFGITIIFFALSHNFWISLALLTVGGMADNVSVIIRGT
                     LLQTLTPQHLLGRVSSVNQIFIGSSNEIGAFESGVAARLLGTVPSVIFGGVMTLVVVA
                     VTMAWSPQLRKMREIDGVSTSTTPS"
     gene            complement(3215..4435)
                     /gene="secF"
                     /locus_tag="VNN25_01635"
     CDS             complement(3215..4435)
                     /gene="secF"
                     /locus_tag="VNN25_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXH90253.1"
                     /translation="MQIFVNSKYDFVRWRFMAVSFSVLFMLVGLAMFFKHGINWGIDF
                     AGGATVTLKFRDAVPMDRLRADLADASIQQYGKAEDRAILIRLPELKQETDYAGQVVA
                     KLIKDLNPEAATKLDLNFQGRDRLAELLYSLDPDAKGTLPTAKDYYRNVAQNVINKRS
                     EVGIFTAISQATSAPGVTTASARILNEKCFLGAFNLLNQETVGPQVGAELQQKAIWAV
                     VLSTLAMGVYLWLRFDLMFGASAIVCIVHDVLMSLAFLMMINGEFSLNIVAALLLIVG
                     FSINDTVVMYDRVRENRRKLKTRMTLEEQLNLAMNQTLSRTILTSGSVVVVLVALILF
                     GGKVIHEFAWILLIGVLAGTYSTVTIVPAVAIAWNNLTGRTHDLSGPATRPRAEARVE
                     TVPPPPQQRKRRVG"
     gene            complement(4457..6025)
                     /gene="secD"
                     /locus_tag="VNN25_01640"
     CDS             complement(4457..6025)
                     /gene="secD"
                     /locus_tag="VNN25_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXH90254.1"
                     /translation="MTRGLLWKVILILVVLVGFAAATIPTQDNPEPIRRGLDLKGGTH
                     LVMRVNVGDAVRLETDQASEMLKAQAKKTNLPIPVTRRVNDTTFVATLPADTPVTPYE
                     TIAKDFLGSFDTGRTPEGLMQFKMKTASQTAIERDTVAHTVETIDNRVNALGVTEPYI
                     APESGYRIVIQLPGVDDPARVKDIIKTTAQLQFRLVEGNPGSDAAAALASVPDNMKTE
                     VDVMPGTKEDALGRAAGIEYYAVRKTVPVTGTDLKNARVQKGQLGQPNVGFSLTPDGA
                     RKFGDLTGANVHRRLAIVLDNKVVSAPVINSQITDSGVIEGSFSAQQAADLSLVLRSG
                     SLPASLTTLEERTVGPSLGRDSIRQGILASIIGFIALVLSVLLYYKGAGINAVLALLL
                     NLVILLGMMAIFHATLTLPGIAGIILTLGMAIDSNVLVFERIREELRDGKTVRAAIEN
                     GFSRAFGTIIDTHMTTIISALFLFQFGTGPIKGFAVTLLIGLFASVFTAFFVSRVIFD
                     VMYKAQNRPTEISI"
     gene            complement(6053..6370)
                     /gene="yajC"
                     /locus_tag="VNN25_01645"
     CDS             complement(6053..6370)
                     /gene="yajC"
                     /locus_tag="VNN25_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXH90255.1"
                     /translation="MTAIALLLQAGSGGTASFLINILPIAAIFLVFYFLVIAPANKQR
                     RKTAEMLSTLKKGDRVLTSGGIYGTIQAVEPEVVYLKIAENVKVKVARSAITGITTGD
                     AAE"
     gene            complement(6411..>6763)
                     /locus_tag="VNN25_01650"
     CDS             complement(6411..>6763)
                     /locus_tag="VNN25_01650"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HXH90256.1"
                     /translation="AAGIDMFDCVMPTRNARNGSLFTSLGKVAIKNARYATDQRPLDP
                     NCSCMTCRTVSRAYLRHLYVNGEIAAMVYNTVHNVSFYLDLMRGIRHSIASNSLQAFR
                     DAFLSRVVSGEVDA"
BASE COUNT         1237 a         1979 c         2242 g         1305 t
ORIGIN      
        1 cgctgtccga cggagccggt agcgccgagg acggcgaccg ggattcgagg ggcgcggcgc
       61 gcgtcagtct gggtgggcaa tgtcagtgtt tcggtcattc agtctcagct ttgtcttcgt
      121 ggattcctgc aagatcgatt ctaagccgaa gaaggcgcct aggtgggcga cgcggatgag
      181 tgtggtccac gcggcgaacg gtccgacgcc gattcctccg aggtgcgcgg tgaacggaag
      241 atgcagcgga ccccaccgaa aaagcatcga tccatgcgcc ggtgacgaag cgcctgcaaa
      301 gtcttggaac aacgcaacct cggtgatcac tacgtcccaa gccacgatcc accatgcggc
      361 caatcgccat cgcgacaacg ccgggaggag gagcgccggc acgacgatgg gtaccagaac
      421 gaaccagtac cacccttctt ttccgccgat cggaacctgg ctgccggccc gttgtgcgag
      481 cacgcagacg atgacgttgt aagtttgcgc gagtgcgaag gcgcctaggg tgagcagagc
      541 cggcgcgaca ttctcgcggc gcagcgccac gatcatagcg atgatgaccg gaccgagata
      601 gagcgcgatt gcagccgggc ggagtgcgtc gttgtgctgc cccgaggtcc aggcggcgct
      661 ggcgatggtg acgcggacga tctcgctgac gttggcgccg gcttcgtgta ccggcccagc
      721 ttgatgagcg agcgccacga cgccgagcgg actgccgcct ggatactgca cgacctcagt
      781 gctcgttctt gccgaaagct cggcgaacgt cagaacgatg gcgatgagcg acacggcgga
      841 gacggcggcg attcgagcct tgcctgcctt ctctcttcgc caaagtaggg gaagcacgat
      901 gagcagcggc cacgtgtaca tcttcgcgtt gagtgccagt ccccacgtca gcgcttccat
      961 ggcgatgatg agcggccggc gtggtgctgc cacgctgatg gcgatggcga ctgcgacgag
     1021 catgcaggcg aaggcatcgt tgctggcgcg aaggatgagc gtttcccagg tcgggagtga
     1081 gacgagaatc gcgccgccga ggattccgat cggaccgatc cagcgccggc cgatctccgc
     1141 ggtcgcgatc accgtgatca gcgcgaaaag gagcgacagc agccgccaca tccgcagttc
     1201 gaagattgcg cttcgcagcg gtacgaggca cgccgtgagc gagtagtaaa tgctcgtctg
     1261 ctgggcttcg tagttgcgga cgacgtatgg cttcacgtcg gcagcagcca acggacgatc
     1321 gtcgagaata tggccgccgc gttccgcaac gatcgagggc cagcggtcgc cagactcacc
     1381 catcgcgggc atggcgatcg cgctagacgt aggtcggact ctccagtccg actgcacccg
     1441 tttgtcggac tgagagtccg acctacgtaa cgcgtcgcct tgagccgccg cgatgctcgc
     1501 gactaagtac ggtgggatcg aaggctcggt cgtcgtcgga ttgcgatgct cggcgcgcac
     1561 gaacgcgagg cgcgccacgt ggtaaatctc gtcgaggccg gcgtacggcg ggagcgcggc
     1621 catctgcagc agaagccgtg cggccgcgca gacgatgagc agcgcgtagg cgattttgaa
     1681 acgcgagttg gcaggcatgc gtgaacagag atcagcgact agagatcaga gatatcagag
     1741 ataccggata aacctgatct cttgtctctg atctctggtg tacgcgcatt ttcacacgtc
     1801 tgcatcgcga tacactccgc gcccgcttgg ccacgcacga tccgtatgca gcgctccgcg
     1861 tccgcaactt cctttggctg atcctcggct acgcaacttc gacggtggcc cgcgaggcgc
     1921 agatcgtcgt tgtcggctgg caggtgtttc aggtcacgcg cgatccgctc gcgctcggga
     1981 tgatcggtct cgccgaggcg ttgccgttca tcgcggtggc gttgtatgcc gggcatgttg
     2041 ctgaccgcct gccgcgtcgt ccgatcgcca tcgtcggcac gttcgggatg ctgctctcgg
     2101 cgatcgcgct gctgctcttc acgaccagcg ggatgatcgc gcgaggccac gtctggccga
     2161 tctacctcgt catctttctg agcggtatct tgcgcagctt catcaggccg gcgttcacgg
     2221 cactgtcagc tgaggtcgtc acacgcgacc tattcgcgaa cgccgttgcc tggcgtagct
     2281 caacgtggca gttcgccgcc gtgctcgggc ctgctattgg tggattgatc tatggctttg
     2341 ccggaccggc tgcggcgtac gcagtcgtga tcgtgctgat gacgctggcc ctcggcgcgc
     2401 tgtggttcat cgcgcacgac gtgcggccgc agcaggcgga agagatcccg atcggcgaga
     2461 gtcttcgcat cggcatccgc ttcctcctcg ggcaacgcat tctcctagca gcgatgacgc
     2521 tcgatctctt ctccgtactc ttcggcggcg cggtggcgct gctgccgatc tttgcgcaca
     2581 tgctcggagc cggaccgcag ggtctgggtg tccttcgcgc agcgccggca gccggctcgc
     2641 tcgtcaccgg catcatcgtc gcgcaccggc gtccgatgcg gcgtaccgga gcagcgctat
     2701 tcgcagcggt ggcaatcttc ggcatcacga tcatcttctt cgccctgtcg cacaacttct
     2761 ggatctcgct ggcgctgctg accgtcggcg ggatggctga caacgtgagc gtgatcatcc
     2821 ggggaacgct gttgcagacg ctgacgccgc aacaccttct cgggcgcgtg tcgtcggtga
     2881 atcagatctt catcggctca tcgaacgaga tcggcgcgtt cgagtccggc gttgcagctc
     2941 gccttctcgg aaccgtaccc tcggtgatct tcggcggtgt gatgacgctg gtcgtcgtcg
     3001 ctgtgacgat ggcgtggtcg ccgcagttgc ggaagatgcg ggagatcgat ggggtcagta
     3061 catcgacgac accatcttga accacagaga cacagagaac acagagatca gatgctgatc
     3121 tccgcggcct ctgtgcctct gtggtgagcg cttcttgaaa acaaaaacgc cgccgaagcc
     3181 ggcggcgttt ttcgtgatga ggacgatgtg attatcagcc cacgcggcgc ttgcgctgct
     3241 gcggtggcgg aggcactgtc tcgacgcgtg cttcggctcg cgggcgggta gccggtccgg
     3301 agaggtcgtg cgtacggcct gtcaggttgt tccaggcgat cgcaacggcg gggacgatcg
     3361 tgacggtcga gtaggtgccg gcgagaacgc cgatgagaag gatccacgcg aactcgtgga
     3421 tcaccttgcc gccgaagagg atcagcgcca cgagcacgac cacgaccgag ccggacgtca
     3481 ggatcgttcg cgacagggtc tggttcatgg ccaggttgag ctgctcttcg agcgtcatgc
     3541 gtgtcttcag cttccgcctg ttctcgcgga cgcggtcgta catgacgacg gtgtcattga
     3601 tcgagaagcc gacgatgagc aggagtgcgg cgacgatgtt cagcgagaac tcgccgttga
     3661 tcatcatcag gaaagccagc gacatgagga cgtcgtggac gatgcagacg attgccgagg
     3721 cgccgaacat cagatcgaaa cgaagccaga ggtaaacgcc catggccagc gtcgaaagga
     3781 cgacggccca gatggctttc tgctgcagct ccgcgccgac ctgcggaccg accgtttcct
     3841 gattgagcag gttgaacgcg ccgaggaagc atttctcgtt gaggatgcgc gcggaggcgg
     3901 tcgtgacgcc gggagctgag gtggcctggc tgatggccgt gaagatgccg acttccgaac
     3961 gcttgttgat cacgttctgc gcgacgttcc ggtagtaatc cttcgcagtg ggcagcgttc
     4021 ccttcgcgtc aggatcgagc gagtagagaa gctcggccag ccggtcgcgg ccctgaaagt
     4081 tgagatcgag cttggtcgct gcttcgggat tgagatcctt gatcagcttg gcgaccacct
     4141 gtccggcgta gtccgtttcc tgcttcagct ccggcaaacg aatgaggatc gcgcggtcct
     4201 ccgctttgcc gtactgctga atcgacgcgt cggccaggtc agcgcggagg cggtccatcg
     4261 gaacagcgtc acggaacttg agtgtgaccg ttgcgccgcc ggcgaaatcg atgccccagt
     4321 tgatgccgtg cttgaagaac atggccagac cgacgagcat gaagagcacg gaaaagctga
     4381 ccgccatgaa gcgccagcgt acgaagtcat atttggaatt gacgaatatc tgcatgggta
     4441 tctctcctcg cctggcctag atgctgatct cggtcgggcg attctgcgct ttgtacatga
     4501 cgtcgaagat cacgcgcgac acgaagaacg cggtgaagac cgacgcgaac agaccgatga
     4561 gaagtgtgac ggcgaatccc ttgatcggac ccgttccgaa ctggaacagg aacaacgccg
     4621 agatgatcgt cgtcatgtgg gtgtcgatga tggtgccgaa cgcgcgcgag aagccgttct
     4681 cgatcgcggc gcgcacggtc ttgccgtcgc gcagctcttc gcggatacgc tcgaacacga
     4741 ggacgttgga gtcgatggcc atgcccagtg tcaggatgat gccggcgatt ccgggcaggg
     4801 tcagggtggc gtggaagatc gccatcatgc cgagcaggat gacgagattc agcagcagcg
     4861 cgagaaccgc gttgattccg gcgcccttgt agtagagcag cacggagagg acgagggcga
     4921 tgaaaccgat gatcgacgcc agaatgccct gtcggatcga gtcgcgtccg agcgacggtc
     4981 cgaccgtgcg ctcttcgaga gtcgtcaacg atgccggcag cgatccgctg cggaggacga
     5041 gagagagatc ggcggcctgc tgcgcgctga agcttccttc gatgacgccg gagtcggtga
     5101 tctggctgtt gatgaccggc gcggatacga ctttgttgtc gaggacgatg gccagccggc
     5161 gatggacgtt ggcgccggtg aggtcgccga acttgcgcgc accatcaggc gtcagcgaga
     5221 aaccgacgtt cggctgtccg agctggcctt tctgcacgcg tgcgttcttg agatcggtgc
     5281 cggtgacggg aacggtcttg cgaaccgcgt agtactcgat gcccgccgcg cggcccagcg
     5341 cgtcttcctt cgtgcccggc atcacgtcga cttccgtctt catgttgtcg ggaacgctgg
     5401 ccagggcggc tgccgcgtcg gagccgggat tgccttcgac gaggcggaac tgcagttggg
     5461 ccgtcgtctt gatgatgtcc ttcacgcggg ccgggtcgtc gacgccgggc aactgaatga
     5521 cgatgcgata gccgctctcg ggtgcgatgt agggctcggt gacgccgagg gcgttgacgc
     5581 ggttgtcgat cgtctcgacg gtgtgggcga ccgtatcgcg ctcgatggcc gtctgcgacg
     5641 ccgtcttcat cttgaactgc atcaaccctt ccggtgtgcg gccggtgtcg aaggagccga
     5701 ggaagtcttt ggcgatcgtc tcgtacggag tgaccggcgt atcggccggg agcgttgcca
     5761 cgaacgtggt gtcgttcacg cggcgcgtca ccggaatcgg cagattggtt ttcttcgcct
     5821 gcgccttcag catctccgag gcctggtcgg tctcgaggcg aacggcgtcg ccgacgttga
     5881 cgcgcatgac cagatgcgta ccgcccttca ggtcgaggcc gcgtcggatc ggctcagggt
     5941 tgtcttgggt cggtatcgtc gcggcggcga agccgacgag gacaacgagg atcaggatga
     6001 ctttccacaa cagacctcta gtcaagattt cctccaggaa gatagggggc ggtcactcgg
     6061 ccgcatcgcc cgtggtgatt ccggtgatcg ctgaacgtgc aaccttgacc ttcacattct
     6121 cggcgatctt caggtagaca acttcgggct caacagcctg aatcgtcccg taaatcccgc
     6181 ccgatgtaag gacgcgatca ccctttttga gcgtactcag catttccgcc gtcttccgcc
     6241 gctgcttgtt cgcgggggcg atgacgagga agtagaaaac aagaaagatt gccgcaatcg
     6301 gcaggatgtt gatgagaaag gaggccgttc cgccggaacc ggcctggaga agcaaagcaa
     6361 tcgctgtcat atcgtttttc tctgtttttc gctcgggggc tctctgtcga tcaggcgtct
     6421 acttcgccgc tcacgacccg cgagaggaac gcatcgcgaa aagcctgcaa agagttggac
     6481 gcgatagagt gccgaatacc tcgcatcaag tcaaggtaaa acgaaacatt gtggacggtg
     6541 ttatagacca ttgccgcgat ttcgccgttc acgtagaggt gccgcaggta ggcgcgggag
     6601 accgtccggc aggtcatgca ggagcagttg ggatcgagcg gacgctggtc ggtggcgtac
     6661 cgtgcattct tgatcgcgac cttgcctaag ctcgtgaaaa gcgagccgtt gcgcgcgttg
     6721 cgcgtcggca tcacgcagtc gaacatgtcg atccccgcgg cca
//
LOCUS       DATLVW010000048         7073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_168210, whole genome shotgun sequence.
ACCESSION   DATLVW010000048 DATLVW010000000
VERSION     DATLVW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168210"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VNN25_01655"
     CDS             complement(<1..363)
                     /locus_tag="VNN25_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90257.1"
                     /translation="MRLFPTLRGRLIAMVAALVAVMIGSVAVISTRVAHYEIRKFDVA
                     VTTGGSPRLPPATSFQIDRLKPPTPGTIAVFYREHGTWAGVQPAVDQMAKTSGTDILL
                     FDRDRRLVASSSNQLRNAR"
     gene            complement(363..1022)
                     /locus_tag="VNN25_01660"
     CDS             complement(363..1022)
                     /locus_tag="VNN25_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH90258.1"
                     /translation="MRRVLIVDDDPKVCETLDRYLAHAGYTTASALDGARALELARTF
                     APDLIVLDLMLPRVDGLEVCRTLRATSQVPIIMLTARTTERDKLAGLALGADDYLTKP
                     FSPRELVARVEAVLRRAGSSERNECVAAGTLVIDPDRCEVTVAGEPVVLTATELRLLL
                     SLARSPGRVFRRDELARLALGDDFDGMDRTIDAHIKNLRRKVGAELIATVFGIGYKLV
                     A"
     gene            complement(1134..1664)
                     /locus_tag="VNN25_01665"
     CDS             complement(1134..1664)
                     /locus_tag="VNN25_01665"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HXH90259.1"
                     /translation="MRTSTRIATLAVLLFATFAWATPPPPCTTIIIVRHAEQTDPTAT
                     DPPLSPAGAARAAALAVALEHSGVQAIYVTQFKRTKQTAARVASLLHVPVTEVPVDLA
                     HVGEYPRQLARRIFTEHPSGTVLVVSHSNTVPGIVSALTNIAVHPLASTEYDRFTVVT
                     AGPSGCQHVLQAQYGH"
     gene            complement(1661..2188)
                     /locus_tag="VNN25_01670"
     CDS             complement(1661..2188)
                     /locus_tag="VNN25_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXH90260.1"
                     /translation="MQSKATTVQEYLSSLPADRREAIERVREVILANLDKDYEEGMAY
                     GMIGYSVPHRIFPSGYHADPRQGLMFAALASQKNYMAVYVMGLYTGCDEANESALVRW
                     FREAWAKSGKKKLAMGKACIRFKKIDDLPLDVLGEAIRRIPAKVYIENYMKARRAIEA
                     AGGARKAAATRHKSA"
     gene            complement(2256..3233)
                     /locus_tag="VNN25_01675"
     CDS             complement(2256..3233)
                     /locus_tag="VNN25_01675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH90261.1"
                     /translation="MKKRTLGKSLEVSSIGLGCMGMSEFYGTGDDAQSIATIHRALDL
                     GCNFLDTADMYGPFKNEELVGRAIRGRRDEVVLATKFGNMRGDDGAFLGVNGKPDYVR
                     NACEGSLKRLGVDHIDLYYQHRVDRTTPIEDTVGAMAELVKAGKVRYIGLSEAAPATI
                     RRAHAVHPVTALQTEYSLWSRDPEDEILPTIRALGIGFVSYSPIGRGFLSSRIKTVDD
                     LEPNDFRRNSPRFKGENFERNLDLVRDIEAIAKAKGVTPAQLAIAWVLARGNDIVPIP
                     GSKSIGHLEENIAAAEITLTASELARIDEAAPKGIAAGDRYASMATVNA"
     gene            complement(3268..4098)
                     /locus_tag="VNN25_01680"
     CDS             complement(3268..4098)
                     /locus_tag="VNN25_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH90262.1"
                     /translation="MRTLILASLLAFTFNLGAQTPETPIGFIPEAIAFKDAPPTLPPG
                     SKVSVLEGNPGAEGMFTMRVRIPAGSAISPHWHPRQERVTVLAGAVDLGFGSVANKSS
                     VTRYRAGSFYLNPPRVVHYLFFPENTELQITGVGPWELMTTDPNEKPAATTVKVRDIT
                     PAPGSELTPTTTIKATIDYDIQNFRPDTYFLDFVFESRVPNRTIGIAPTVVPYRKDAT
                     QTHFVESATGTITLIQDLGRFLDLPDLKRPIRMRVYVHEKRSETSNHVVGMSDWIEFH
                     "
     gene            4179..4832
                     /locus_tag="VNN25_01685"
     CDS             4179..4832
                     /locus_tag="VNN25_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HXH90263.1"
                     /translation="MNCNRYVGTVQTEWLPDGRRMKLLQTLQYIDPACNTWEAPKEWV
                     VDGASIPQFAWSIIGGPFEGKYRNASVIHDVACDQKTKPWEAVHEVFYNAMIASGVSI
                     VTAKIMYAAVYHFGPRWPQTVKAVVRSRDVDFHITSDTEDIRRMASRETNASPWVLND
                     SLPGAEDQDESAVNVTIEPPPPSLAPEDFEQLRRAIEQRGPDAAESMTLEEIRSYQP"
     gene            4917..5423
                     /locus_tag="VNN25_01690"
     CDS             4917..5423
                     /locus_tag="VNN25_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HXH90264.1"
                     /translation="MNNHAHGHGLRRRDEIQRIVREQSVHSQEELLALLKKRGFRVTQ
                     PTLSRDLRELGVAKSPNGYITHDAFAGGAVVAFAPRDVVETRLEQAVAEYVTGADTAG
                     NLVVIKTPAASAQPVASAIDAAQIDGVLGTIGGDDTIFVAFRTTDAATTFAQRLRRTI
                     GVRRRNVR"
     gene            5445..6512
                     /gene="argC"
                     /locus_tag="VNN25_01695"
     CDS             5445..6512
                     /gene="argC"
                     /locus_tag="VNN25_01695"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXH90265.1"
                     /translation="MNEPISFADRVDAAAAPVAAAPKKRVAIVGATGYSGAELTAIMA
                     RHHGVDLVALFSSSSTARGPVTPTLPKLIAEPFTLDALIAANPEVVFLCTPNEVSAEL
                     APKLLDAGMTVIDLSGAFRLEEPSMYPTWYGFAHENPKLLGEAVYGLTEYCNGELKKA
                     RLVANPGCYPTSILLALRPLNFAIDREQPVICDSKSGVSGAGKKADVNFSFAELFGNF
                     KAYGVGNHRHEPEIRQQLHLGDRAPLVFVAHLLPVHRGILSTMHVGFTHAMTEQQITD
                     AYARVYENAPFVRVLPAGQLPELKNVVNTPRAEIGFQLLQGGRRAVIVSVIDNLLKGA
                     ASQAVQNFNRMFDFAETEALI"
     gene            6512..>7073
                     /gene="argB"
                     /locus_tag="VNN25_01700"
     CDS             6512..>7073
                     /gene="argB"
                     /locus_tag="VNN25_01700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXH90266.1"
                     /translation="MNVIKLGGSLLDDASRRAEVLGAIAKRWKSGEQIVLVHGGGKHV
                     DANLKRLGIAKRTHAGLRITDDATLDVVVSTLAGSVNKMLVSELAAMGVRAAGFSGCD
                     ASTLIAELHEPIEGVALGHVGRVTGGDPALINAVIGAGIMPILSSVAEGPNGSLLNVN
                     ADTAAAAIAIAVGAEKLVFITDVAGLL"
BASE COUNT         1297 a         2153 c         2280 g         1343 t
ORIGIN      
        1 ccgcgcgttg cgaagttggt tgctcgacga cgccaccagc cgccggtcac gatcaaagag
       61 aagaatgtcg gttccagaag tcttcgccat ctgatcgacg gccggttgca cgccagccca
      121 cgtgccgtgc tcgcggtaga acacggcaat tgttcctggc gtaggcggct tcagccggtc
      181 aatctgaaac gacgtagcgg gcggcaagcg aggactccct ccggtcgtca cggcgacgtc
      241 gaacttacgg atctcgtaat gcgccacgcg cgtcgagatg acggccacgc tgccgatcat
      301 gacggcgacg agcgccgcga ccatagcgat gaggcggccg cgcagtgtgg gaaagagccg
      361 catcaggcca ccagtttgta gccgatgccg aagacggtgg cgatgagctc cgcaccgact
      421 ttgcggcgca gattcttgat gtgcgcgtcg atcgtgcggt ccattccgtc gaagtcgtcg
      481 ccgagtgcga ggcgggccag ttcgtcgcgg cggaagacgc ggccggggga gcgcgccaac
      541 gagaggagca gccgcaactc ggtggcggtc agcacgaccg gctcgccggc gacggtgact
      601 tcgcaacggt cgggatcgat caccagcgtg cccgccgcga cgcactcgtt cctctccgac
      661 gatccggccc ggcgcagcac agcttcgacg cgtgccacga gctcgcgcgg gctgaacggc
      721 ttggtcaggt aatcgtcggc gccgagggcg aggccggcga gtttgtcgcg ctcggttgtc
      781 ctggccgtca gcatgatgat cggaacctgc gaggttgccc gcaacgtgcg gcagacttcg
      841 agcccgtcaa cacgaggcag catcaggtcg agcacgatca agtcgggagc gaacgtccgt
      901 gccagttcca gcgcccgcgc gccatcgagc gctgatgccg tcgtgtagcc ggcatgcgcc
      961 agatagcgat cgagcgtctc gcaaactttc ggatcgtcat cgacgatgag gacgcggcgc
     1021 atggctccat cgtagggtta attgtgaatt tggggtgaag gctgtcacgc gaagacgcga
     1081 agacgcgaag atggcgatgg cctcttcgtg tcttcgcgcc ttcgcgtgac aactcaatgc
     1141 ccgtattggg cctgaagcac gtgctggcag ccggaagggc ccgcggttac gacggtgaac
     1201 cggtcgtact cggtgcttgc aagtggatgg accgcgatgt tcgtcagcgc gctcacgatt
     1261 ccggggacgg tgttgctgtg gctgactacg agaacggtgc cgctgggatg ctccgtgaag
     1321 atgcgacgcg cgagttgacg cggatactcc ccgacatgcg cgagatcgac cggaacctcg
     1381 gtcacaggaa cgtggagcag cgatgcgacc cgcgccgcgg tttgcttcgt ccgcttgaat
     1441 tgcgtgacgt agatggcctg cacgcccgag tgctcgagcg ccacggccag tgccgccgcc
     1501 cgcgcggctc cggcgggact cagtggagga tcggtggccg taggatctgt ctgctctgcg
     1561 tggcgaacga tgatgatcgt cgtgcaggga ggcggtggcg tggcccaggc gaaggttgcg
     1621 aacagaagga cagcgagtgt ggcgattcgt gtggaagttc tcatgctgat ttgtgcctcg
     1681 tcgctgccgc cttacgggct ccgcctgcgg cctcgatggc gcggcgtgcc ttcatgtagt
     1741 tctcgatgta gaccttcgcc ggtatccgcc ggatcgcttc gcctaggacg tcgagcggca
     1801 gatcgtcgat cttcttgaat cggatgcacg cctttcccat ggcgagcttc ttcttgccgc
     1861 tcttcgccca ggcttcgcgg aaccagcgaa cgagtgcgct ctcgttcgct tcatcgcacc
     1921 cggtgtacaa acccatgacg tagacagcca tgtagttttt ctgcgaggcg agcgcggcga
     1981 acatcaatcc ctggcgcgga tcggcgtgat agccggaggg aaagatccgg tgcggcaccg
     2041 agtagccgat cattccgtag gccatccctt cttcgtaatc cttgtcgaga ttcgcgagga
     2101 tgacttcgcg aacgcgctcg attgcctctc gtcgatcggc aggcagggag gacaggtatt
     2161 cctgaacggt cgtcgccttg ctttgcatgg ggaaagtata ggtccgcaga tgacgctcga
     2221 cgcagatcca ctgatctttc gtctggggac agggctcacg cgttcacggt ggccatgctc
     2281 gcgtaccggt caccggcggc gatacctttc ggcgccgcct catcgatccg cgccagttct
     2341 gaagcagtca gcgtgatctc agccgcggca atgttttcct cgaggtggcc gatgctcttg
     2401 ctgccgggga tcgggacaat gtcgttgccg cgagcaagca cccatgcgat ggcgagctgc
     2461 gcgggcgtga caccttttgc ctttgcgatc gcttcgatgt cgcgcacgag gtcgagattg
     2521 cgctcgaagt tctcgccttt gaatcgcggc gagttgcggc ggaagtcatt cggctcgaga
     2581 tcgtcgaccg tcttgatgcg gctcgagaga aatccgcggc cgatgggcga ataggaaacg
     2641 aagccgatgc cgagcgcgcg tatcgtcggc aggatctcgt cttccggatc gcggctccag
     2701 agcgagtact cggtctgcag cgcggtgacc ggatgcacgg catgtgcgcg gcgaatcgtg
     2761 gccggcgccg cctcggacag tccgatgtac cggacctttc cagccttcac cagctccgcc
     2821 atcgccccaa ccgtatcttc gattggcgtc gtgcggtcga cgcgatgctg gtagtagaga
     2881 tcgatgtgat cgacgccgag tcgcttgagg gagccttcgc acgcgttgcg aacgtagtcg
     2941 ggttttccgt ttacgccgag aaacgcgccg tcgtcgcccc gcatgttgcc gaacttcgtg
     3001 gccagaacga cttcgtcgcg gcggccgcga atggcgcggc cgaccaactc ttcattcttg
     3061 aacggaccgt acatgtcggc cgtatcgaga aagttgcacc cgaggtcgag cgcgcgatgg
     3121 atcgtggcga tggattgggc gtcgtcgcct gtgccataga actccgacat gcccatgcat
     3181 ccgaggccga tggaggagac ttcgagagac ttaccgagcg tgcgtttctt catggtgcaa
     3241 acgtagcagc aaacggagtg ccgctggtca gtggaactcg atccaatcgc tcatcccaac
     3301 cacgtggttc gaggtctcgg accgtttttc gtgaacgtag acgcgcatgc ggattgggcg
     3361 cttcagatcg gggagatcga ggaagcgccc gaggtcctga atcagagtga tggttccggt
     3421 cgcgctctcg acgaaatgtg tttgggtggc atcttttcga taagggacga cggtgggggc
     3481 gatgccgatc gttcgattgg gcaccctcga ctcgaagacg aagtcgagga agtaagtgtc
     3541 gggccggaaa ttctggatgt cgtagtcgat cgttgctttg atcgtcgttg tcggggtcag
     3601 ctcgctgccc ggcgccggag tgatgtctcg caccttgacc gtcgttgcgg ccggcttctc
     3661 gttcgggtcg gtcgtcatca actcccacgg accgacgccg gtgatctgca actcagtgtt
     3721 ttcagggaag aagaggtagt gcaccacgcg cggcgggttc agatagaagc tgccggcgcg
     3781 atagcgagtg acggagctct tgttcgcaac ggaaccgaac ccgaggtcga cggcgccggc
     3841 gagaacggtg acgcgctcct ggcgcggatg ccagtgcggc gaaatcgcgg agccggccgg
     3901 aatgcgcacg cgcatcgtga acatcccctc ggcgccagga ttgccttcga gcacggacac
     3961 tttcgatccc ggcggcagcg tcggtggcgc gtctttgaat gcgatcgcct cggggatgaa
     4021 cccgatcgga gtctccggcg tttgcgcgcc gaggttgaag gtgaacgcca ggagcgacgc
     4081 gaggatcaat gtgcgcatct attaatgata gttgcgggcc gcaccgatgc gataattcgc
     4141 gcacaaccaa caacgctgcg tgaagccggg ggtgcgtgat gaactgcaac cgatacgtgg
     4201 gaaccgttca aaccgaatgg cttccggatg ggcgccgcat gaagcttctg cagacgctgc
     4261 agtacatcga tcctgcctgc aacacttggg aagcgccgaa ggaatgggtc gtcgacgggg
     4321 cgtcgatccc tcagttcgca tggtcgatca tcggcggtcc gttcgaaggc aagtatcgaa
     4381 acgcatcggt gattcacgac gtcgcatgcg accagaaaac gaagccgtgg gaagcggttc
     4441 acgaagtctt ctacaacgcc atgatcgcct cgggagtcag catcgtcact gccaagatca
     4501 tgtatgcggc ggtgtaccac ttcggtccgc gctggccgca aacggtgaaa gcggtggtca
     4561 gaagtcgtga tgtcgacttt cacatcactt ccgacacaga ggatattcgg cggatggcgt
     4621 cacgcgagac gaacgccagc ccatgggtac tgaacgactc cttgccaggc gcggaagatc
     4681 aggatgagtc ggccgtaaac gtgacgatcg aacctccgcc tccatcgctg gcgccggaag
     4741 attttgagca gttgcgtcgc gccatcgagc aacgaggtcc tgacgcggct gaatcgatga
     4801 cgctcgaaga gatccgaagc taccagccct agtcccgctc ccgacgcccc acattcggaa
     4861 taccaggctc acaaccctgt tgacatccat cgaaggatga ataaaaattc gactcgatga
     4921 ataatcatgc gcatgggcac ggactgcgga gacgcgatga gatccagcgg atcgttcggg
     4981 agcagtccgt tcattcgcag gaggagttgc tggcgctgct gaagaaacgc ggcttccgcg
     5041 tcacgcagcc gactctctcg cgcgacctgc gcgagctcgg cgtggccaag tcgcccaatg
     5101 gctacatcac ccatgacgcg ttcgcgggcg gcgctgtcgt cgcctttgcg ccgcgtgacg
     5161 tcgtcgagac gcggctcgaa caagccgttg ctgaatatgt caccggcgcc gataccgccg
     5221 gcaacctcgt tgtaatcaag actccggccg cgagtgcgca gccggtcgcc agcgccatcg
     5281 atgccgcgca gatcgacggc gtcctcggca ccatcggcgg cgacgacacc atcttcgtcg
     5341 ccttccgcac caccgacgcc gccactacct tcgcgcagcg cctgcgacgg accatcggcg
     5401 tccgccgccg caacgtccgc tgagcgcgtt cccagagaat tgccatgaac gaacctattt
     5461 catttgctga tcgtgttgat gccgccgcgg cgccagttgc ggctgcgccg aagaagcgcg
     5521 tcgccatcgt tggcgccaca ggctactccg gcgcggagct gaccgcgatc atggcgcgcc
     5581 atcacggcgt tgatctcgtc gcgctttttt cgtcgagcag caccgcgcgc ggcccggtca
     5641 cgccgacgct tccgaagctc atcgccgagc cgtttacgct cgacgcactg atcgctgcga
     5701 atcccgaggt tgtttttctt tgcacgccga acgaggtgtc cgcggagctc gcgccgaaac
     5761 tgctcgacgc cggcatgacc gtcatcgatc tttccggcgc atttcgtctc gaggagccgt
     5821 cgatgtatcc gacctggtac ggcttcgcgc acgagaaccc gaaacttctc ggcgaggctg
     5881 tctacggact caccgaatac tgcaacggcg aattgaagaa agcgcgtctc gtcgccaatc
     5941 ccggctgcta tccgacgtcg atccttctcg ctttgcgtcc gctcaatttc gcgatcgacc
     6001 gcgagcagcc ggtgatctgt gattcgaaga gcggagtctc tggagcgggc aagaaagcgg
     6061 acgtgaactt ctcgtttgcg gaattgttcg gcaacttcaa ggcgtacggc gttggcaatc
     6121 atcgccacga gcctgagatc cgccagcagc ttcacctcgg cgaccgagcg ccgcttgtct
     6181 tcgtcgcgca tttgctgccg gtgcatcgcg gaatcctctc gacgatgcac gtcggcttca
     6241 cgcacgcgat gaccgagcag cagatcaccg atgcgtatgc gcgggtatac gagaacgccc
     6301 cgttcgtgcg tgtgctgccg gccggccaac tgccggagct gaagaacgtc gtcaacacgc
     6361 cgcgcgcgga gatcggcttc cagctcctgc agggcggccg ccgcgcggtg atcgtttcgg
     6421 tcatcgacaa cctgctcaaa ggcgcagcct cgcaggccgt acagaacttc aaccgcatgt
     6481 tcgattttgc ggaaacggag gccttgatct gatgaacgtc atcaaactcg gcgggagcct
     6541 tctcgatgat gcgtcgcgcc gcgccgaagt gctaggcgcg attgccaaac ggtggaagag
     6601 cggcgagcag atcgtgctcg tacacggcgg cggtaagcat gtcgacgcga atctcaagcg
     6661 cctcggcatc gcgaagcgca cgcacgcagg tctgcgcatc accgacgacg caacgctcga
     6721 tgtcgtcgtg tcgaccctgg ccggatcggt gaacaagatg ctcgtatcgg agctcgcggc
     6781 gatgggcgtc cgcgcggcag gcttcagcgg ctgcgatgca tcgacgctca tcgccgaact
     6841 gcacgagccg atcgagggcg tcgcgctcgg tcatgtcgga cgcgtcaccg gcggcgatcc
     6901 tgcgctcatc aacgccgtca ttggcgccgg catcatgccc attctctcgt cagtcgccga
     6961 aggcccgaac ggatcgctgt tgaatgtgaa cgcggacacg gccgctgccg cgatcgcgat
     7021 cgcagtcgga gccgagaagc ttgtcttcat caccgacgtc gccggtctgc tcg
//
LOCUS       DATLVW010000049         5666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_168228, whole genome shotgun sequence.
ACCESSION   DATLVW010000049 DATLVW010000000
VERSION     DATLVW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168228"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="VNN25_01705"
     CDS             <1..1729
                     /locus_tag="VNN25_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXH90267.1"
                     /translation="TLIRTTTAGIAKATVTASSAGPVVVNATVNNVTKSITINFQDAV
                     IPPNPTPTTPTISLVDPSFGLPTGNQTVTITGTNFRPPVRVLFDPGSGQAPKEGFVTS
                     VTPTTITVTTPAFDLGVSQQLIVAITVIVEAGSPTEQRVTKAAAFTYTAPVLTPVFRA
                     LSPTSGSIDGGTRIVITGDAFEAPIQVFFGSAQAQVLSVTFHEIDVISPTARETNPNG
                     SGTVTGPVDIRILNVNSGKSVSVPGAFRYINKMQITSSSPTFGSALGGTNIIIDGIGF
                     TDGAVVDVGDVRASVIKVTGTEVTARTSPIAVPCASTGALTIKVTNIDNGDSATGGTF
                     SYIGVPSVITSITSSGSPTVGSALTVNVQNPGVGLLGTAIIAFTVGGQPANVTPLQIS
                     TGTGSQAFTVIVPPGLTFPTGSCLVGGFPGTQQLPGNFEVRFTNTTTGCTAATTITIN
                     PTAAGAACVVPPTAAVTSPAQTCPVANLTPPSVVAAGPATQTANITITNNGTQQLVLG
                     PPTVTPNNATLISIVPNSAQNVPGGGSANYTITLDPTAAGATGATITFTTNDPTKPTL
                     TVTVCGNAT"
     gene            1839..3122
                     /locus_tag="VNN25_01710"
     CDS             1839..3122
                     /locus_tag="VNN25_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH90268.1"
                     /translation="MAQPGINAKLEELQFRLKTDPKSRLFFPLAEELRRHGKLAEAEQ
                     VLRNGLHVHTSYLSAWVSLGRVLREQGKNAEAVEALTTAMQVDPGNVVAARLLADAYL
                     DLGEKVEAIKKYKLVNALMPSEELEEKIVNLDREIQAPAPSVQESVPESRQEPVPEPI
                     APKPQSDAPPESPFAAEESPSPFAEGEPAHATELVGAAWEEEVPSAPSVHASDAQTPE
                     VTRDAQKPEVTQAEPYHYTVETPFDTGDDEPMAARHVVSPFEDPAGYSAAALEVEQPE
                     GMHIEETPVEHEASDPWSETQPEAFPESEPAAEVFAPAEPEPAPSADADGDVTATTTM
                     ADLYVRQGLIEDARKIYEQVLQRHPDDGEVRAKLEALTASAPAVAAAHEEPVNDEPIY
                     DEPIDEPVATIAGSPKVRVLQQWLAKVGRREEGRA"
     gene            3115..3480
                     /locus_tag="VNN25_01715"
     CDS             3115..3480
                     /locus_tag="VNN25_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90269.1"
                     /translation="MHSGVFRDALESIRDHVEGTLAVSLIALDGIAIESITSDNVPLD
                     VMGAEFGGFIKSVRLSNTDLHTGDVLQFSLFTEEYITLLSAVTPEYFLLLVMQPDGNY
                     GRARHELAKAKFALRDELV"
     gene            3492..3992
                     /gene="accB"
                     /locus_tag="VNN25_01720"
     CDS             3492..3992
                     /gene="accB"
                     /locus_tag="VNN25_01720"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXH90270.1"
                     /translation="MNFKEILELIDKVADRGIGGVEIEQAGLKVRIDGKAAAQPQVIH
                     SYGDRSSAPAAPPAPAPIAPAPPTAAEKAATAKAEEEAAEAGMHIITSPIVGTFYRSA
                     NPEAEPFVNVGDRISKGQVLCIIEAMKLMNEIESDVDGVIATIYPQNGQPIEYGEKLF
                     AVKLSS"
     gene            3997..5349
                     /gene="accC"
                     /locus_tag="VNN25_01725"
     CDS             3997..5349
                     /gene="accC"
                     /locus_tag="VNN25_01725"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXH90271.1"
                     /translation="MPTISKVLIANRGEIALRVILACKELGLKTVAVHSEADRDALHV
                     RYADDDVCIGPAASRQSYLNISSIIAAAEITGADAIHPGYGFLAENAHFAEIAGECGL
                     TFIGPTPESIRLMGNKAVARDTAKKAGVPILPGSDGPVRTFEEARDVAQTIGFPVILK
                     ASAGGGGRGMRICNDETSLQSAFDTARNEAERAFGSADVYIEKYLEHPRHIEIQVFGD
                     THGRVVHLGERECSIQRRHQKLLEESPSPVLTQELRHAMGEAAIKLCKAVDYVNAGTI
                     EFLFQDGEFYFMEMNTRIQVEHAVTEEVTGMDLVKEQIRVASGLPLTVPQHEFRLRGH
                     AIEFRINAEDPVTFAPNPGKIREFHIPGGPGVRVDTAAYRDYVIPPHYDSLIAKLIVR
                     GKDRSEALARGRRALEQFIIEGVKTSIPLHRAIVNNDRFIRGEFSTRFMEEFEWGREG
                     "
     gene            5483..>5666
                     /locus_tag="VNN25_01730"
     CDS             5483..>5666
                     /locus_tag="VNN25_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014879889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXH90272.1"
                     /translation="MKIFVVGFMGAGKTTVGRELAARLAAPFFDLDELIESGEQTSIK
                     ELFAQQGEPYFRKRERE"
BASE COUNT         1128 a         1824 c         1712 g         1002 t
ORIGIN      
        1 cactctgatc cggaccacca cggcaggcat agccaaagcg acagtcacgg caagcagcgc
       61 cggtccggtc gtcgtgaacg cgaccgtcaa caacgtgacg aagagcatca cgatcaactt
      121 ccaggacgcg gtcattcccc ccaacccgac accgaccacg ccgacgatca gcctcgtcga
      181 ccccagcttt ggattgccga ccgggaacca gaccgtgacc atcaccggca cgaacttccg
      241 tccgccggtg cgcgtccttt tcgatccggg cagcggacag gcgccgaagg agggattcgt
      301 cacaagcgtc actccgacta cgatcacagt cacgacgcct gccttcgacc tcggcgtgag
      361 ccagcagctc atcgtcgcca ttacggtcat cgtcgaagcg ggctcgccca cggaacaacg
      421 ggtgaccaag gcggctgcct tcacctacac ggcacccgtt ctgacgccgg tcttccgcgc
      481 cctctcgccg acgtccgggt cgatcgatgg cggaacacga atcgtcatca ccggcgatgc
      541 cttcgaagct cccatccagg tcttcttcgg atcggcgcag gcccaggttc tcagcgtcac
      601 cttccacgag atcgacgtca tatcgccgac cgcgcgcgaa acgaatccaa acggatccgg
      661 caccgtcacc gggcccgttg acatccgcat tctcaatgtc aactcgggta agtccgtgtc
      721 cgtccctggg gcgttccgat acatcaacaa gatgcagatc acttcttcgt cgccgacatt
      781 cggcagcgct cttggcggca cgaatatcat catcgacggt attggcttca ccgatggggc
      841 cgtcgttgac gtcggtgatg ttcgcgcctc ggtgatcaag gttacaggga ccgaggtcac
      901 ggcgcgtacg tcgccaattg ccgttccgtg cgccagcaca ggggcgctta ccatcaaggt
      961 gacgaatatt gacaatggcg actccgcaac gggcggcaca ttcagctaca tcggcgttcc
     1021 ctccgtcatc acatcgatca catcgtcggg aagtccgacg gtgggcagtg ccctcacggt
     1081 caacgtccaa aaccctggcg tcgggctcct cggtaccgcc atcatcgcgt ttaccgttgg
     1141 aggtcagccg gccaacgtaa cgccgttgca gatctcgacc ggaactggct cgcaggcctt
     1201 cacggtcatc gttccccctg gtctgacgtt cccgacgggt tcctgcctgg taggaggatt
     1261 ccctggcaca cagcaactgc cgggcaactt cgaggttcgg ttcaccaata cgacgacggg
     1321 ctgtacggcg gcgaccacga tcacgatcaa cccgacggcc gcgggagcag cgtgcgtggt
     1381 gcccccgacc gctgccgtaa cgtcccccgc gcagacgtgt ccggtggcca acctcacgcc
     1441 gccctccgtc gttgcggcag ggcccgcgac gcagactgcg aacatcacga ttacgaacaa
     1501 cggaacgcag cagttggtgc tcggtcctcc gacggtgacc ccgaacaacg ccacgctgat
     1561 ttcgatagtg ccgaactccg cgcagaacgt gcccggtggc ggcagcgcga actacacaat
     1621 cacactcgac ccaacggctg ccggggcgac cggcgcgacg atcaccttca caacgaacga
     1681 tccgacaaag ccaacgctca cagtgacggt ctgcggaaac gcaacatagt gtagaaagca
     1741 ggaccaacca ccgaaccgcc ccgcacgccg gggcggttcg tttctgcggc cgaggttgcg
     1801 ccgcccgcgg ttgttgaaca gaattcgaac ttcagcacat ggcccaaccc ggtatcaacg
     1861 ccaaactcga agagctgcag ttccggctca agaccgatcc gaagagcagg ctctttttcc
     1921 cgctcgcgga agagctgcgg cgacacggga agctcgctga agccgaacag gtgctccgca
     1981 acggcctcca cgtgcatacg tcctaccttt ccgcctgggt gagcctcggc cgcgttctcc
     2041 gtgagcaggg caagaacgcc gaggcggtcg aggccctgac gacggccatg caggtcgatc
     2101 cgggaaacgt cgtcgccgcc cgcttgctgg ccgacgccta cctggacctc ggcgagaagg
     2161 tcgaggcgat caagaagtac aaactcgtga atgccttgat gccttcggaa gagctcgagg
     2221 agaaaatcgt gaatctcgac cgtgagattc aggcaccggc gccttcggtg caggaatcgg
     2281 tgccggagtc acggcaggaa ccagtgccgg agccgattgc tcccaagccg cagtctgatg
     2341 ctccgccgga gtcaccgttc gccgccgaag agtcgccctc gccgttcgcg gagggggagc
     2401 cggcgcacgc tacggagttg gtcggcgcgg catgggagga ggaggtgccg tccgctccct
     2461 ccgttcacgc gagcgatgct cagacgcccg aggtgacccg ggacgctcaa aagcctgagg
     2521 tgacccaggc tgagccttat cactacaccg tcgaaacgcc gttcgatacc ggcgacgatg
     2581 agccgatggc tgcccgccac gtcgttagtc cattcgagga tcctgcgggc tattcggcgg
     2641 ctgcactgga agtcgaacag cccgagggta tgcacatcga ggagacgcct gtcgagcacg
     2701 aggcatccga tccgtggtcc gagacgcagc cggaagcatt cccggagagt gagccggcgg
     2761 ctgaagtatt tgcccctgcc gagccggagc cggcgccttc agcggacgcg gatggtgatg
     2821 tcacggccac gacgacgatg gcggacctct acgtgcgcca gggcttgatc gaggacgcgc
     2881 gcaagattta tgagcaggtg ttgcagcgtc atcccgacga tggcgaagtg cgcgccaagc
     2941 tcgaagcgct cactgcctcg gcaccggcgg tcgccgctgc tcacgaagag ccggtcaacg
     3001 atgaaccgat ctacgatgaa ccgatcgatg agccggttgc gacgattgcc ggctccccga
     3061 aagtgagagt tctgcagcaa tggctggcca aagtcgggcg gcgggaggaa gggcgtgcat
     3121 agcggcgttt ttcgtgacgc actcgaatcg attcgcgacc acgtcgaggg taccctggcg
     3181 gtcagcctca tcgcgctcga cggtatcgcc atcgagtcga ttacgtccga taacgttcct
     3241 ctcgacgtca tgggtgccga gtttggcggc ttcatcaaat cggttcgcct gtcgaacact
     3301 gaccttcaca ccggcgacgt tttgcagttc tccctattca ctgaagagta cattaccttg
     3361 ctctcggcgg tcaccccgga gtactttctg ctgctcgtga tgcagccgga cggaaactat
     3421 ggccgcgcgc ggcacgagct ggcgaaggcg aaattcgctc ttcgcgatga gctggtctga
     3481 gggatcgaat tttgaatttc aaagagattc tcgaactgat cgacaaagtt gcggaccgcg
     3541 gaatcggcgg cgttgagatc gaacaagccg gtctgaaggt gcgcatcgac ggcaaggcgg
     3601 ccgcacaacc gcaggtcatt cactcgtatg gcgatcggag cagcgctccg gccgctccgc
     3661 ccgcgccggc gcccatcgct cctgcgccgc ctacggctgc ggagaaggcg gccacggcca
     3721 aggcggagga ggaagccgcc gaagcgggga tgcacatcat cacctcaccg atcgtaggca
     3781 cgttctaccg ctcggccaat ccggaagcgg aaccgttcgt caacgtcggc gaccgcatct
     3841 ccaaggggca ggttctctgc atcatcgagg cgatgaagct gatgaacgag atcgagtcgg
     3901 atgtcgacgg tgtcatcgcc acgatctacc cgcagaacgg tcagccgatc gagtacggcg
     3961 agaaactctt tgcggtgaag ctctcctcct gacgctatgc cgacaatctc gaaagtcctg
     4021 atcgccaacc gaggcgagat cgcgctacgc gtcatccttg cctgcaagga gctggggctc
     4081 aagaccgtcg cggtgcattc ggaggccgat cgcgacgcgc tccacgtccg ctatgccgac
     4141 gacgacgtct gcatcggacc ggcggcctcg cgccagagtt acctgaacat ctcctccatc
     4201 atcgccgcgg ccgagatcac cggcgccgac gccattcatc ccggctacgg cttcctcgcg
     4261 gagaacgcgc acttcgccga gatcgccgga gagtgcggtc tcacgttcat cggcccgacg
     4321 cctgagtcga tccggctgat ggggaacaaa gccgtggccc gcgacacagc gaagaaggcc
     4381 ggcgttccca tcctgccggg tagcgacgga ccggttcgca cgttcgagga agcccgcgat
     4441 gtggcccaga cgatcggatt ccccgtcatt ctcaaggcct cggccggcgg cggcggacgt
     4501 ggcatgcgca tctgcaacga cgagacgagt ctccagagcg cgttcgatac cgcacgcaac
     4561 gaggccgagc gcgccttcgg ttcggccgac gtctacatcg aaaagtatct cgagcatccg
     4621 cgccacatcg agattcaggt cttcggcgat acccacggcc gcgtcgttca cctcggcgaa
     4681 cgcgagtgct ccatccagcg ccgccatcag aaacttctcg aggagtcgcc atcgccggtc
     4741 ctgacccagg agttgcggca tgcgatgggc gaagcggcga tcaagttgtg taaggcggtc
     4801 gattacgtca acgcggggac gatcgagttc ctctttcagg atggcgagtt ctatttcatg
     4861 gagatgaaca cccgcatcca ggtcgagcac gccgtcacgg aagaagtcac cggcatggat
     4921 ctggtgaaag agcagatccg cgtcgcttcc ggtttgccct tgaccgtacc gcagcacgaa
     4981 ttccgcctcc gcggccacgc catcgagttc cgcatcaacg ccgaagatcc ggtgacgttc
     5041 gcgccgaacc cgggaaagat ccgggagttt cacatcccgg gcggcccggg agtccgcgtc
     5101 gacactgccg cataccgcga ctatgtgatc ccgccacatt atgactcgct gatcgccaag
     5161 ctgatcgtga gagggaagga tcgctctgag gcgttggctc gcggccggcg agcactggag
     5221 cagttcatca tcgaaggtgt gaagacgtcg attcctcttc accgcgcgat cgtcaacaac
     5281 gatcgcttca ttcgcggcga gttctccacc cggttcatgg aagagttcga gtgggggaga
     5341 gaggggtgag agacagagat cagcgacaag agattagaga tcagaccagc tccgttgatt
     5401 cctgatctct tgtctgtgat ctcctatctc tgatttctgc tgtcctgatc tcttatctct
     5461 tgtctctgat ctctgctcca acatgaaaat cttcgtcgtt ggattcatgg gcgccggcaa
     5521 gactacggtc ggccgggagc tcgcggcgcg tctggcggct cccttcttcg acctcgatga
     5581 gctcatcgag tcgggcgagc aaacatcaat caaggagctc tttgcgcagc agggtgagcc
     5641 ctattttcgc aagcgggagc gtgagg
//
LOCUS       DATLVW010000050         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_1782, whole genome shotgun sequence.
ACCESSION   DATLVW010000050 DATLVW010000000
VERSION     DATLVW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VNN25_01735"
     CDS             complement(<1..252)
                     /locus_tag="VNN25_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90273.1"
                     /translation="MAVSQKEYDHSVFINCPFDRDYNALFDAIVFTITDCGFRPVCAR
                     ERMNSAEIRLDKIVDLIRDSRYSVHDLSRTEVDEKYSLPR"
     gene            complement(272..526)
                     /locus_tag="VNN25_01740"
     CDS             complement(272..526)
                     /locus_tag="VNN25_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90274.1"
                     /translation="MNVRKKSSSRPVVAKKPAKRPVKKAVAAKEPARSTGKRISTREA
                     EREAMNHVISRPYLSPLGKREISSRRIIAAIKAVMKAQGS"
     gene            623..2173
                     /locus_tag="VNN25_01745"
     CDS             623..2173
                     /locus_tag="VNN25_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90275.1"
                     /translation="MTRRLAAAALFVLIAIAAVAPIRSYDYFWHLTTGRWIVEHHSIP
                     TVDPFAVASAQVPWINGEWLYEIVLYAVHGIAGDPGISIISALLAAAIFAAGFVFATR
                     ENAVGPALLIAAIAFAGASDRLGVRPAAAAALLIVIALGILSSHMRLTPLVIAYACVT
                     VVWINVHPSAILAPVLALITMLIDVRRWTVAIASAAALLINPFGWNAILSPLRLSSTI
                     RSGAFVNTEWLPSSFSYFPLLYVVTGVLALLFVLTPDKRANAWRFVTFAILGALAFRY
                     VRNQGLYFAALPLLLPPLGNVSRRVSGVLAAIALAPLAWVFQHDVHRLGVDDERFPMR
                     AVAALHSYQLDGNIYNADQFGGLLEWTFYPLRCALTDGRNELFGDFIAADAAAHRDSR
                     AWHALIAKYDARLAVDEYQTQRMQVIDGLTGEKRLLPASLVRYRRHDWALIAFDDAAM
                     VFARRNAFPPAQLAAIEYRYLVPDDPSIGYTTTEIRDAARAEVVRARAQFGDIRVVNE
                     LERGIAAN"
BASE COUNT          359 a          693 c          661 g          469 t
ORIGIN      
        1 ccgcggcaag ctgtacttct catcgacctc ggttcgcgac agatcatgca ccgagtagcg
       61 cgaatcgcga atgaggtcga caatcttgtc gagtcggatt tcggcggagt tcatgcgctc
      121 gcgcgcgcag accgggcgga atccgcagtc ggtaatcgtg aacacgatcg cgtcgaagag
      181 cgcgttgtag tcgcggtcaa acgggcagtt gatgaatacg ctatggtcgt actccttctg
      241 cgagacggcc atgaggctaa ttctcgaagt cctacgagcc ctgggccttc atcactgcct
      301 taatcgccgc gatgattcgc cgggaactga tctcacgttt gccaagcggc gacaaatagg
      361 gacgcgagat gacatgattc atggcctcgc gctctgcctc gcgagttgag atgcgtttac
      421 cggtggagcg agcgggttcc tttgccgcca ccgctttttt taccgggcgt ttcgcgggct
      481 tcttggcgac gacaggtcgt gaagatgatt tcttgcggac gttcattttc gtgatggcaa
      541 ttatacgcgc atcgttgtaa ccagagcacc gctcgtggac ctcggtacac agcccagctc
      601 cgatgactat actcgcgacc ccatgacgcg acgtctcgcc gcagccgccc tcttcgtcct
      661 catcgccatc gccgcagtcg cgccgattcg cagctacgac tacttctggc atctgacgac
      721 cgggcgctgg atcgtcgagc atcattccat tcccacagtc gatccgttcg ccgtggcgag
      781 cgcgcaggtg ccgtggatca atggcgagtg gctgtacgag atcgtgctct acgcggtgca
      841 cggcatcgcg ggtgatcccg gtatctcgat catcagtgcg ttgctggcag cagcgatttt
      901 cgcggcggga ttcgtgttcg cgacgcgcga gaatgctgtc gggccggcgc tgctcatcgc
      961 tgccatcgca ttcgccggcg cttccgatcg attgggcgtt cgtccggccg ccgcggcagc
     1021 gcttctcatc gtcatcgccc tcggcatact cagttcgcac atgcgcctca cgccgctcgt
     1081 gatcgcgtac gcgtgcgtga cggtcgtctg gatcaatgtg cacccatcgg cgattctcgc
     1141 gccggtgctg gcgctcatca cgatgctgat cgatgtgcgc cgctggactg tcgccattgc
     1201 cagcgcggcg gcgctcctca tcaatccctt cggatggaac gcgatcctgt cgccgttgcg
     1261 gctctcatcc acgattcgca gcggcgcgtt cgtcaacacc gaatggctgc cttcgtcatt
     1321 cagctacttc ccgctgctgt acgtcgtcac tggggtactg gcgctgctgt ttgtgctaac
     1381 gcccgacaag cgggcaaacg cctggcgctt cgtcaccttc gccatcctcg gcgcgctggc
     1441 gtttcgctat gtgcgcaacc aaggtctcta cttcgccgcg ctgcctttgc ttttgccgcc
     1501 gctcggcaac gtttcgcgcc gcgtttccgg cgtattggct gcaatcgcgc tcgcgcctct
     1561 tgcctgggtc ttccagcacg atgtgcatcg cctcggcgtc gatgacgaac gattcccgat
     1621 gcgggccgtc gccgccctcc attcgtacca gctcgacggc aacatctaca acgcagatca
     1681 gttcggcggg ctgttggaat ggacgttcta cccgttgcgg tgcgcgttga cagacgggcg
     1741 caacgagctg ttcggagact tcatcgccgc ggatgccgcc gcgcaccgcg acagccgcgc
     1801 gtggcacgcg ttgattgcga aatacgacgc acgtctggcc gtcgacgagt atcagacgca
     1861 aaggatgcag gtcatcgacg gcctcacagg cgagaagcgc ctgttacccg catcgctcgt
     1921 ccgctaccgc cgccacgatt gggcgctcat cgcgtttgat gatgcggcga tggtgttcgc
     1981 gcggcgcaac gcgtttccgc cggcgcaact tgcggcgatc gagtaccgct atctcgtgcc
     2041 tgacgatcca tcgatcggat acacgacgac ggagatccgc gacgcggcga gggctgaggt
     2101 tgttcgcgcc cgcgcgcagt tcggggacat cagagtggtg aacgagttgg aacgcggcat
     2161 cgcggcgaat tagccaaaaa ag
//
LOCUS       DATLVW010000051         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_126918, whole genome shotgun sequence.
ACCESSION   DATLVW010000051 DATLVW010000000
VERSION     DATLVW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126918"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..457)
                     /locus_tag="VNN25_01750"
     CDS             complement(149..457)
                     /locus_tag="VNN25_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXH90276.1"
                     /translation="MTDFIRICKQSEIPQPGDVREFTANGLALCVANLDGTICVLNGT
                     CPHEEGPLGEGTIESGRIVCPWHGYAFDLRTGEAEGEPTLKAQVFEAKIEGGELRAKL
                     "
     gene            complement(574..2301)
                     /locus_tag="VNN25_01755"
     CDS             complement(574..2301)
                     /locus_tag="VNN25_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXH90277.1"
                     /translation="MAATTDSNPESSTPRPEEPRTSNFLRGMIAEDLRTQKFGNATIQ
                     TRFPPEPNGYLHIGHAKAICLDFGLADEVGGRTNLRFDDTNPEKEEQEYVDSIQADVR
                     WLGFEWERLCYASDYFGQLYEWALKLVRDGKAYVDDLTADEIRQHRGTLTEPGKNSPY
                     RDRSVEENLDLFTRMKAGEFPDGARVLRAKIDMTSPNINLRDPIMYRILHASHHRTAD
                     EWCIYPMYDYAHGQSDSIENVTHSMCTLEFADHQPLYRWFIDNLGIFPSQQIEFDRLN
                     LTYTLLSKRKLLQLVNEKRVSGWDDPRMPSLSGIRRRGFTPEGIRTFVNSLGATRTNG
                     IVDIEMLEHFQRDDLNHRASRAMAVLRPLKIVIDNYPEGQSEFVEVQNNPEDPSAGTR
                     QVPFSRELYIEQDDFREVPPPKYYRLSPGKEVRLRSAYFITAQSVSKDAAGNITEVRC
                     TYDPASRGGNSPDGRKVKSTMHWVSALHAITAEVRVYDKLFTTPDPQAVPEGKTFLDN
                     LNPNSLEVIADAKLEPSLANAKPGDHYQFERVGYFTADPDSKEGAPVFNRTLPLKDTW
                     AKIEKKAGA"
BASE COUNT          484 a          680 c          971 g          615 t
ORIGIN      
        1 tgaattggaa gctgctggtg gggaaagctg gaaagtgagc aataagcgat gaagagaata
       61 agtgattaag tgaacaggtg acacccatcc tacgcgggaa ttgagaaatc ccaggtcccc
      121 gagtgcgagg gacctggggg aaccccggtt atagttttgc ccgcagttct ccgccttcga
      181 tctttgcctc aaagacctgc gccttgagcg tgggctcacc ttccgcttct ccggtgcgga
      241 gatcgaatgc atagccgtgc catgggcaaa cgatgcggcc gctttcaatg gtcccctcgc
      301 ccaggggccc ttcttcgtgg ggacaggtgc cgtttagcac gcagattgtt ccgtcgagat
      361 tggctacaca aagcgctagt ccatttgccg tgaactcgcg tacgtcgccg ggctggggga
      421 tttcagattg tttgcagatg cggatgaagt cggtcatgat tcctctctct gagagattcc
      481 aaacgcggtg tactcagaat cgtagcctcc caccctcgcg acagtaaaaa gtcgcgagaa
      541 ttgggcacgc gacatgcgat gggaccgtca agttcaggca ccggccttct tctcgatttt
      601 cgcccaggtg tcttttagcg ggagcgtgcg gttgaagacg ggtgcgccct ctttgctgtc
      661 ggggtcggcg gtgaagtagc ctacgcgctc gaattggtaa tggtcgcctg gtttggcgtt
      721 ggctagcgaa ggttcgagct tggcgtcggc gatgacttcg agcgagttgg gattgagatt
      781 gtcgaggaag gttttgcctt cggggacagc ttgcgggtcg ggcgtggtga agagcttgtc
      841 gtagacgcgg acttcagcgg tgattgcgtg cagcgccgag acccagtgca tggtggattt
      901 caccttgcgg ccgtcgggcg agttgccgcc tcggctggcg gggtcgtagg tacagcggac
      961 ctcggtgatg ttgccggcag cgtctttcga cacgctttgc gcggtgatga agtaggcgga
     1021 gcggaggcgg acttccttgc cgggcgagag gcggtagtac ttgggcggag ggacttcgcg
     1081 gaagtcgtcc tgctcaatgt agagctcgcg gctgaagggg acctggcgcg tgccggcact
     1141 gggatcctcg ggattgttct ggacctcgac gaactccgac tggccttcag ggtagttgtc
     1201 gatgacgatc ttgagcgggc gcagcacggc catggcgcgc gaggcgcggt ggttgaggtc
     1261 gtcgcgctgg aagtgttcga gcatctcgat atcgacaatg ccgttggtgc gcgtggcgcc
     1321 cagcgaattg acgaaggtgc ggatgccttc cggcgtgaag ccgcggcggc gaatgccgga
     1381 gagcgagggc atgcgcgggt cgtcccagcc gctgacgcgc ttctcattca cgagttggag
     1441 gagtttgcgc ttggagagca gcgtataggt caggttgagg cgatcgaact cgatctgctg
     1501 cgacgggaag atgccgaggt tgtcaatgaa ccagcggtag aggggctggt ggtcggcaaa
     1561 ctcgagggtg cacatggagt gggtgacgtt ctcgatcgag tcggactggc cgtgcgcgta
     1621 gtcgtacatg ggatagatgc accactcgtc ggcggtgcgg tgatgcgagg cgtggaggat
     1681 gcggtacatg atggggtcgc gcagattgat gttgggcgat gtcatgtcga tcttggcgcg
     1741 gaggacgcga gcgccgtcgg ggaattcgcc ggccttcatg cgggtgaaga ggtctagatt
     1801 ttcctcgacg gagcggtcgc ggtaaggcga gtttttgccg ggttcggtga gggtgccgcg
     1861 gtgctggcgg atctcatcgg cggtgaggtc atcgacgtag gccttgccgt cgcggacgag
     1921 cttgagcgcc cactcgtaga gttggccgaa gtagtcggag gcgtagcaga ggcgctccca
     1981 ttcgaagccg agccagcgga cgtcggcctg gatggagtcg acgtactcct gctcttcttt
     2041 ttcggggttg gtgtcgtcga accggaggtt ggtgcgacca cccacttcat cggcgagccc
     2101 gaagtcaagg cagatcgcct tggcgtggcc gatgtggagg tagccgttgg gctcgggcgg
     2161 aaagcgcgtc tggatggtcg cgttgccgaa cttttgcgtg cgtaggtctt cggcgatcat
     2221 gccgcggagg aaattggagg tgcggggttc ttccgggcga ggcgttgagc tttccgggtt
     2281 cgagtctgtc gttgctgcca tgggtctatt gtttcatttt tgcgcgacga aacggaattc
     2341 agctaggctc tatccccttt gtcctcgcat aacgggaaca acgccttgct cttcggtgtc
     2401 taaggcaata tgcgcacagt caagttgttc tgcgtgattg ccgcagttta cttggtcggt
     2461 acagcggtct tcttgtttgc gtacgggcag actctgaaga agcgcgcaga ggcgctaatt
     2521 ctcacctcat acgacttgtc caactggacg ggaaggcctc ccacactagc cgtccttgaa
     2581 agcaagctcc gcgggcagct aaagcctatg tcaggttgca ggtcgtcgga atgtagctat
     2641 gaggtacgcg tgtcgaacgg tgggctggcc gcgctcaaga tggctcccta tgcggaattg
     2701 agcgcggagt atgcggttcg agacgggata gtgcaatcga gcatgctgga
//
LOCUS       DATLVW010000052         6758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_249903, whole genome shotgun sequence.
ACCESSION   DATLVW010000052 DATLVW010000000
VERSION     DATLVW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249903"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..800
                     /locus_tag="VNN25_01760"
     CDS             162..800
                     /locus_tag="VNN25_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HXH90278.1"
                     /translation="MTALAEYREPRVGRATEPGLRNIAARFGTQSYCDCGTAPELSMA
                     GVAPYTLEAWIKPRSVTGGFIVGKLSEGSLSIVRGHLVAGRNVGDRGVGSVAIRSTTV
                     LETGRWYHVATTFDGMSMTLYVNGRPEVRTRVLTRLKGFSSEATLIGAQKNQELLCNF
                     FLGLIGEVRIWNVSRTAGQMLTWMDTVEPAASGLVAHYGSWSNPEGRHVNHS"
     gene            1233..1355
                     /locus_tag="VNN25_01765"
     CDS             1233..1355
                     /locus_tag="VNN25_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90279.1"
                     /translation="MTEIEAFVVYDNAGRRYWLYLGNWLKRPLATRRLRRYIEP"
     gene            1445..2545
                     /locus_tag="VNN25_01770"
     CDS             1445..2545
                     /locus_tag="VNN25_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HXH90280.1"
                     /translation="MKTLFPEVRPIAEHAKRWAEVTDRLRILRNATCYTILTTGRIAA
                     KIELCKDVHSIQLALDCALRRQGELLGDNANAKEARRKHSKQFVHLSSAAMTLSELRQ
                     LLTRTPSGAAVSQEDLRYMVATLENLSRSQRLVSRFLRKSPTHWIIPSLPARDAASIR
                     QGQDNLPPDPDDIPYRLHRQVYNTEIPAAEACAAMLVRFADAPEGAQNALARQVSDEG
                     RHAAVVLAKLLQEATRKKQYPINFSIWSNAYGATTLAEAFCIEQVIGEGFGLGNDLFM
                     AARYRRLKRTDLAEMQSWIHADEVNHARVGIQWFRQLAGAEASELLSHLEKQFVHKPI
                     AGRWFMRALRCKIGFTHEEIDRQHRMAQKQVP"
     gene            2561..4087
                     /locus_tag="VNN25_01775"
     CDS             2561..4087
                     /locus_tag="VNN25_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90281.1"
                     /translation="MRLISQAEESAIGDMLEHSDPAIQKRGLQRLCSQLQEGRLLVNR
                     ALIEPRLMNLRWSADIKVRSWLYNTIARLRTRSFRDYLFHQIVFGETDAENITWAVAA
                     FFSSALPDEVAQLLKQPALTFFDTALELAAQLYYDGPILGRIEQKPFEKAVGYDAIFA
                     KWYALLFGYDKVDRRIPEFTFPHVEYIGALTSHHDDDVAEYSIWSLFMSRTADRSAVQ
                     LQPAEVGARNPHIRRWYYRLIAKSAADVLANADFFKSRIATEQDVIAREGLAAGLGRN
                     YVPSLAADMLTWFMLEEDELVRLAMAPHFAMYAAVDPAYEELVAAVVSRTEDAPDLTS
                     KVLIATAERVRPTTHVTQLLTEREGRLARRSGLVVNIRGAIDMSDNSKSVTNIAHGGN
                     VNVGSINLGDLENSSLEVISRIDAAGPQSAELKLTLDRLVRELTAATEITADDKEVVH
                     AVVRDIAEYQRVPQERGRTLLQKSVATLRGVSSALPAAAAVATEIHAVIDAIMRLLAP
                     "
     gene            complement(4563..5021)
                     /locus_tag="VNN25_01780"
     CDS             complement(4563..5021)
                     /locus_tag="VNN25_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HXH90282.1"
                     /translation="MGQTIYWAGPLFTQAERMWNRRCADELRTRGFTVVLPQEEATAF
                     MIKGKADLKRIAENCYQQAITSDILVAVLDGADCDSGTGVEIGLRIGDRKTPSRAGVI
                     GVLTDFRVIERGGPNAMLTLVDDIVSVSSLHDDLNELISALESAILRLSR"
     gene            complement(5390..5744)
                     /gene="ssrA"
                     /locus_tag="VNN25_01785"
     tmRNA           complement(5390..5744)
                     /gene="ssrA"
                     /locus_tag="VNN25_01785"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5799..6644
                     /gene="nadC"
                     /locus_tag="VNN25_01790"
     CDS             5799..6644
                     /gene="nadC"
                     /locus_tag="VNN25_01790"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXH90283.1"
                     /translation="MSSHDSERNAEHAMHEIVRRALEEDLPDITSEAIFAPGERGQAR
                     FLVKTPGVLAGLQYAAAVFSALDPEAVFIPTRNDGDSVEAGDVVAEVGASVIGLLGGE
                     RTALNLMQRASGIATATRRYVDAVRGTNAKIYDTRKTAPGLRALDKYAVRCGGGENYR
                     MGLYDMFLIKNNHVDRAGSITAAVERIRSRSMPQKIMVEVRDTLELDEALALRPDFIL
                     LDNMSVAQMAEAVARVGGSVPLEASGGVNLDTVRAIAETGVDRISIGALTHSVVAMDI
                     SMRIL"
BASE COUNT         1432 a         1946 c         2006 g         1374 t
ORIGIN      
        1 ccccacagcg ccggcacacg aggcgtgcga ggcgatcgat cagctccatg ttgtcctcga
       61 ggaagatctc tctctcacga cggaaaaacg tccgggcccg tcgcactctc ttgtcattga
      121 ccgcctggtc tgcaacgaac ggcggcgacg aggagagaac gatgactgca ttggccgaat
      181 atcgggaacc acgcgtaggg cgagccacag agcctgggtt gcgcaacata gccgcacgtt
      241 tcgggacgca gtcctattgc gattgcggca ccgccccgga gctgagcatg gccggcgtcg
      301 ccccgtacac cctcgaggcg tggatcaagc cgcgctccgt caccggaggc ttcatcgtcg
      361 gcaagctctc cgagggctcc ctctcgatcg tccggggcca cctggtcgcg ggccgcaatg
      421 tgggagaccg gggagtggga tcggtggcga tccgctcgac cacggtgctc gagaccggcc
      481 ggtggtacca cgtcgccacg acctttgacg gaatgtccat gaccctctac gtgaacggca
      541 ggccggaagt gcgcacgagg gtgctcaccc gtctgaaagg attctcgagc gaggccacgc
      601 tcattggcgc acaaaagaac caggaactgc tctgcaactt ctttctgggg ctgatcggcg
      661 aggtccgaat ctggaacgtc agccgcacag ccggccagat gctcacgtgg atggacaccg
      721 tggagccggc cgcatcggga ctcgtggctc actacggctc ctggagcaac ccggagggac
      781 gtcacgtcaa ccattcgtaa gggggcgcgc ttcaggtgcc gtcgaaaggt ggccaacgtc
      841 agctcccacc taccttcgcg cctacacgaa cgatccacat aaagggtaat cttcttaggt
      901 gatcgtctgc cggagaaggc ccatgagtgg accgcgctga tcggagggat tctcggcacg
      961 ctgctcggcc tttcaagctt cgtcctcgcg tggcttaata ccgacgcgat cgggcacggc
     1021 tccgcctcgt agctagtgac gacatgggcg agtatgccca ccgtcggctc aacctctcga
     1081 tcacgaccat cggacgccga cctatccaga tcacgtcccc ttggatcttc tagaagaaca
     1141 cccggcgggg tgaccccatc gaaccatggc gtgaagagcc gcttctcctc acggagcatc
     1201 aacatatctt cgagttcgca aatgacattc ccttgaccga gatcgaagca ttcgtcgttt
     1261 atgacaacgc gggccgtagg tactggctct acctcgggaa ctggctcaag aggcctctcg
     1321 ctactcgcag gttgaggcgg tacatcgagc cgtaggagca gttcctacct aggtttcggt
     1381 aggattactt caacctctcc gccgtcgtat gatacgttga ccttctaccc gacaggacta
     1441 agccgtgaag acactctttc ctgaggtgcg cccgatagcg gaacacgcca aacgatgggc
     1501 agaggttacc gaccggctac gcattctccg caacgccact tgctacacga tacttacgac
     1561 tggcaggatc gccgcaaaga tcgagctttg taaggatgtg cattctattc agttggcact
     1621 ggactgtgct ttgcgccgcc aaggcgaact gttgggagat aacgcaaacg ccaaagaagc
     1681 acgccgaaag cactcgaaac aatttgtgca cttgtcctca gcggccatga ctctgtccga
     1741 gctccgccag ctattgacca gaaccccgtc gggcgcggct gtatcacagg aggacctgcg
     1801 gtacatggtc gcgacgctcg agaaccttag ccgctcgcaa cgcctggtca gccgcttctt
     1861 gcggaagtca cccacgcatt ggatcattcc ctcgttgccg gcccgggatg cagcatctat
     1921 acggcaaggc caggataatt tgccacccga tccagatgat atcccgtacc ggctgcaccg
     1981 ccaggtttac aacacagaga ttcctgcagc ggaggcctgc gcggcaatgc tcgtgcgatt
     2041 cgccgacgcg ccagagggag cacaaaacgc gttagcccgc caggtttcag acgagggcag
     2101 acacgccgcg gtggtgctcg caaagctttt gcaggaagca actcgcaaga agcagtaccc
     2161 aatcaatttc agtatctggt ccaacgccta tggtgccacc acgcttgcgg aagccttctg
     2221 catcgaacaa gtcatcgggg aagggttcgg acttggtaac gaccttttca tggcagcacg
     2281 atatcgacgc ctaaagcgta ctgacctagc cgaaatgcag agctggatac acgccgacga
     2341 ggtaaatcac gcccgggtag gtatccaatg gtttcggcaa ctcgcgggcg ccgaggcctc
     2401 cgagcttctc agtcacctcg aaaagcaatt cgtccacaag cccattgcgg gacgttggtt
     2461 catgcgtgcg ttgcgttgca agatcggctt tacgcatgaa gaaatcgatc ggcaacatcg
     2521 aatggcgcag aaacaggtac cctaagactt caacaggacc gtgcgactaa tctcccaggc
     2581 agaagaatcc gccatcggcg atatgctgga gcactcagac ccggctattc aaaagcgagg
     2641 tctccaacgt ctctgtagcc aactgcaaga gggacgcctc ctcgtcaacc gagccttgat
     2701 cgaaccgcgg ctcatgaacc ttcgatggtc cgccgacatt aaggtcaggt cttggctata
     2761 caacacaatc gcgaggttgc ggacgcgttc atttcgcgac tatctctttc accagatcgt
     2821 gttcggtgag accgatgcag agaacattac ctgggcggtc gccgccttct tctcgtcagc
     2881 gttgcccgac gaggttgctc agctgctgaa gcagcccgcg ctgacgttct tcgatacggc
     2941 attggagtta gcagcgcaac tatactatga tggacctatt cttgggagga tagaacaaaa
     3001 gccctttgag aaagctgtcg ggtacgatgc cattttcgcc aaatggtatg ccctgttatt
     3061 cggctatgac aaggttgatc gtcgcattcc agaatttaca ttcccacacg tcgaatatat
     3121 cggtgcgtta acgagtcacc acgacgacga cgtcgcagag tattcgatct ggtccctctt
     3181 catgtcgaga acggctgacc gatctgcggt ccaattgcag cctgctgagg tcggtgccag
     3241 gaatccgcac attcggcgct ggtattatcg gttaattgcg aagtcggccg ccgacgtgct
     3301 cgcaaatgcg gatttcttca agtctcgcat cgccaccgag caagacgtta ttgcgcgcga
     3361 gggattagcg gctggccttg gaagaaacta cgtcccctcg ctggccgctg atatgctcac
     3421 gtggttcatg ctggaggagg acgaactcgt ccggctggca atggcgccac actttgcaat
     3481 gtatgctgcc gtcgatccgg cttacgagga actcgtggct gccgtggtga gtaggaccga
     3541 ggatgcccct gatctcacca gtaaggttct gattgcaacc gccgaacgcg tccgccccac
     3601 cacgcacgta acacagttgt tgaccgagcg cgagggtcgg ttggcaaggc ggagcggact
     3661 cgtcgtaaac atccgaggag caatagatat gagcgataat agcaaatcgg ttaccaacat
     3721 agcgcacggt ggtaatgtca atgtcggcag tatcaacctt ggtgacctcg aaaactcgtc
     3781 gctggaagtg atttcccgaa tcgacgcagc gggcccgcag agcgctgaat tgaagctcac
     3841 gctagataga ctcgtgcgcg aacttacggc ggctacggag attacggcgg acgacaagga
     3901 ggttgtgcac gcggtagtgc gtgacattgc ggagtatcag cgcgtcccgc aggagcgagg
     3961 acgaacgctg ctacaaaaat cggtcgctac gttaagagga gtcagttcgg cgttgccggc
     4021 ggcagcagcc gtcgcgaccg agatccacgc tgttatcgac gctattatgc ggttattggc
     4081 gccatgacag aatcagtaat tggtgataca aatctgacga tgacggacgt gattcttagt
     4141 tcaagcgcgc gcttttatcc gatagtcatc gaacttaggg acgagctcca acgagccggg
     4201 ctatccgtgc aaacgccgaa tttgaagttt gttgcggcat cggtatcgcc cacgcgcaag
     4261 cggcagttga cggtcgactt tctcgcgaag atatcgaaga gtcgcgtggt gtgcgtcgtt
     4321 acggacgcta gtggatatgt tgggcgaagc gtcagcttag aggtcgggtt cgcctatgca
     4381 atccgtaaac cgctcgtatg cttgcaagac cttgaggatc cggcgatttc ctgcctgtgc
     4441 aagaagctgt cgtcggcgaa cgcgctgatt cggtttacgc gagcgcttcg atcacgaagc
     4501 cgcgtctctc aatgaaatgg ttgagacgac ggaataatgg cgtagtctgt tggagcgaga
     4561 aatcatcgac tcaaccgtag tatggcgctc tcaagagcgc ttatcagttc gtttagatca
     4621 tcgtggagcg acgagacaga gacgatgtcg tcgacaagcg tcaacatcgc gtttggtccg
     4681 ccgcgttcta tgacgcggaa gtccgtcaga acaccgataa cgcccgcccg gcttggcgtc
     4741 ttgcgatcgc caatccggag tcctatctcc acaccggtgc cactatcaca gtctgcgccg
     4801 tccagaactg cgactagaat atcgctagtg atcgcctgct ggtagcagtt ttctgctatc
     4861 cttttgaggt cggccttccc tttgatcatg aaggcggtcg cttcttcctg cggcaacacg
     4921 acagtaaatc ctcgcgttcg taattcgtcg gcgcaccgcc tgttccacat tcgctcggct
     4981 tgtgtgaata agggtcctgc ccagtagatc gtctgtccca ttatcgctcc aagtagttag
     5041 cagaggctcc gccgaaggcc attagctcgt gcggctcagc aacagtagct caatcgcaga
     5101 gtaggcaggt actaagagtt cttccgggga ctccaatttt gggccgtggg gtgacgctct
     5161 ttgtgcagcg gatcgtctca ccgcgctgag ttccctgcac caagtccggc cggcacagcg
     5221 gcggtaggta cggaagatat acgggcagga ttccggtgtg gccgtagact tgaaacatcg
     5281 cgctctccgt tctaaatcga agagtacgta ttaggtacac cgccgaaacg ctggattctc
     5341 ctgatttgct aaacccctct ggaagagtgg tttaggttgt gaaaatcgtt ggtggaggcg
     5401 gcgggagttg aacccgcgtc cgagagcagt gaagcctggc tactacatgc ttatctctcc
     5461 cttggttctc gtccactttc tgcccggaga gcggggaatg aatgtggacc agcccctggg
     5521 ttttcgtcgt ctgcgccggg acggttcgca gacgcttagc tcaccttaat gacatccggc
     5581 accgacggcg tgagcaacca tcgggcggac gctcgtgggt ttttaggccg cgagagcgag
     5641 ttggctgttg ttcgcagcta ctcaggttga tcagatttac gaggtaatca gctcggcatg
     5701 caaccatgac cccgacatgt ccccgtcgaa gccggtacgc ccccaaattt tcaacgatct
     5761 gcgcgacaaa acccctccgc tgatggcagc attcctgtat gagctcacac gattctgaac
     5821 ggaacgccga gcacgccatg cacgagatcg tgcgccgcgc gctggaggaa gatcttcccg
     5881 acatcaccag cgaggcgatc ttcgcgcccg gcgaacgcgg gcaggcgcgc tttctcgtca
     5941 agacgcccgg agtgctggcc ggactgcaat acgcggcggc ggttttcagc gctctcgatc
     6001 cggaggccgt cttcatcccg actcggaacg acggcgactc ggtcgaggcc ggggatgtgg
     6061 tggccgaggt gggggcgtcg gtgatcgggt tgctgggggg agaacgcacg gcgctcaacc
     6121 tcatgcaacg cgcctcgggc atcgccacag ctacgcgccg ctacgtcgat gcggtccgag
     6181 gaacgaacgc gaagatttac gacacgcgca agaccgcgcc gggactgcgc gcgctcgaca
     6241 agtacgcggt gcgctgcggc ggcggcgaga actaccgcat ggggctctac gacatgttcc
     6301 tgatcaagaa caaccacgtc gacagagccg gttccatcac cgcggcagtc gagcggatcc
     6361 ggtcaaggtc gatgccgcag aagatcatgg tcgaggtgcg cgacacgctg gagctggacg
     6421 aggctctggc gctgcggccc gacttcatcc tgctcgacaa catgagcgtc gctcagatgg
     6481 ccgaggcggt ggcgcgggtg ggtgggagcg tgccgctgga ggcgtcgggg ggggtcaatc
     6541 ttgacacggt tcgcgcgatc gcggagacgg gggtcgatcg gatctcgatc ggagcgctga
     6601 cgcactcggt ggtggcgatg gatatttcga tgagaatttt gtgattccgg tgcgagggtc
     6661 aagagaaccg tttcacgcgg agacgcggag gtcgcggagg tggtttctcc gcatctccgc
     6721 gtctccgcgt gaaaccaaag aatcgaaatc acttggca
//
LOCUS       DATLVW010000053         8502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_44546, whole genome shotgun sequence.
ACCESSION   DATLVW010000053 DATLVW010000000
VERSION     DATLVW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44546"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VNN25_01795"
     CDS             <1..537
                     /locus_tag="VNN25_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXH90284.1"
                     /translation="GGFDFPGVAASLTTLLPSSITSRTIDFSKTSSAHADVVNGMNNG
                     SLLTTYVGHASVEMWANSVFLSSDASVLTNGTRLPVVFAMNCLNGYFHDLYTESMAEA
                     LLRAPNGGAVAVWASSTLTEPDQQSLMSHELFRQLFRTDVNLTLGDAVTRAKFAATDP
                     DVRKSWILFGDPTMKLRP"
     gene            complement(547..3744)
                     /locus_tag="VNN25_01800"
     CDS             complement(547..3744)
                     /locus_tag="VNN25_01800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HXH90285.1"
                     /translation="MRSSASSADQKKSRPYAELRSASAFSFLDSASLPEDLIEHAARK
                     DIPAMALIDTNGVYGAPRFYGAAKKAGVRAIVGAEVVLGNEQQKPGLTLTRMDAARSN
                     ARETRLTLLVESHTGYRNLCKLLTAGALDRPKAQARFTWDLIAQYAEGLHCITGGDEG
                     PLAHALTKNGVDDAEKLLHKISGIFKGRTHIELQRHHRRDEEHRNIALVDFARRFHLP
                     LIATNGVRYARAEDKELHDVLTCIREGKHVDSAGRLLGVNRERFIKGADEMAMLFADL
                     PEALDGAWKLANTLDFTLANLGYQFPEYPVPDGESPMSFLRKIAWNGATTRFRPLTAK
                     AQAQIEKELATIEKLDLAGYFLIVWDIVQFCQREKILVQGRGSAANSAVCYALGITAV
                     DPVSMELLFERFLSEERGEWPDIDLDLPSGDQRERVIQHVYQTYGVYGSAMTANVITY
                     RDRSSAREVGKALGFSLEQADKLSKTLGTWNFGEIRERMESMPQELAGAGFDPADLRV
                     QHFMRLQLQIQNLPRHLGQHSGGMVMARGRLDEVVPLEPAAMPGRVVIQWDKDDCADL
                     GIIKVDLLGLGMLAAIEEMIPLIRTNEGIDVDLAHLPQDDKRVYKMLNEADTVGMFQV
                     ESRAQMASLPRNAPAKFYDIVVQVAIIRPGPIVGGMVKPFFDRRQGKAPLEYPHPCLE
                     PILRRTLGVPLFQEQLLRIAMVAADFTGGEAEELRRAMGFKRSMERMGEIEKRLRSGM
                     TKNGIDLPAQDQIVKSITSFALYGFPESHAASFALIAYASAYLKAHHPAAFYIALMNA
                     WPMGFYHPATLIKDAERHGVTVLPIDVNHSGWHCRWERGAVRLGMKYVKGLRETAARS
                     IEASSPFRNADDLARRAMLRDDQLTKLAFAGALASLGLKRRASLWQAAQAAKAAGELF
                     ESIAPSSTESPLDEMAPGEETLADYAAMDLTTGPHLLSHFRPQLKRDGVYSATQLKKL
                     PNGKRVATAGAVIVRQRPGTAKGFVFLTLEDETGISQAIVKPDLFRDHRALIVGSPGL
                     IVEGILQNEDGQCSVRADKFWSLEGIGDVASHDFH"
     gene            complement(3795..5432)
                     /locus_tag="VNN25_01805"
     CDS             complement(3795..5432)
                     /locus_tag="VNN25_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HXH90286.1"
                     /translation="MTRLACFYVPMFALAARLRSEPDLLHEAVGIVEGNGNNAHIVAA
                     TRRARQKGIHPGLSLTQARSLLPKLIARGRDAECERTAQEALLEVAESFSPRVEDAGE
                     GVVFLDVMGMERHFVDAMSDVGRTLQSDISSSRTRESDLRTPEHLLARAAIRACEAVS
                     LPARVGIAASKLAARVAAELPKSPNVIAPGEERTFLAPLPLTRLSPELEAAAMLQRFG
                     LSSIGDLARLPESEIASRLGEIGRELHWAARGIDPRPLMPRAMPPEFREGMELEWPLV
                     ALEPFLFIANAALDRLAKRMEIQGFACKRLEFTLTLDPDGYDARGIDLPAPTRDVKTM
                     LTLMRLDLEKTPPGAPVVGFSFVAHPDKPRRAQLSLFGPTAMSPEKLATTIAKLVALL
                     GDGRVGMPMTVDGHLPERFAISDFDPPPPPDVRRKPSRAHGLLAVRTFRPPIAIEVTT
                     RETADKETQIASIRTIANSLPPSTPHSTKRIEVGATIEIAGTVRICSGPWRVEEGWWS
                     DRPDVREYWDVELERGGVYRLFRGVSEDWFVDARYEM"
     gene            complement(5429..6337)
                     /locus_tag="VNN25_01810"
     CDS             complement(5429..6337)
                     /locus_tag="VNN25_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90287.1"
                     /translation="MVESALRPLPDPPPIPPGEHDAIRNLRASLEASIASKLTSGSEL
                     IRALARQRREEILPTTIEPLDALLGGGLSRGKMTEIAGRGTRFSIVIAALAAATSIGE
                     AAALIDAGDCFDPQLAEAAGVDLQRLLWARPRTMKQMVTAAEMLGATGFQLVIVDMAT
                     RRPPGRRVPDAAWVRLARVAESHGATILVSAPYALTGTTSEAMILAYAAHARWLGRGN
                     APRVLAGIETRLKLEKHRRMRPGQSVSVFFRCEEAIRGGRESGVGSRIQNPNTSSPSA
                     SQLQGRDPLKAFSDSRLPTCDTRKAS"
     gene            6606..6899
                     /locus_tag="VNN25_01815"
     CDS             6606..6899
                     /locus_tag="VNN25_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXH90288.1"
                     /translation="MNGEYDSTTTVEIFGQTYNVRGEGDPNYLTELARFVDSRMREVA
                     AQVATVDPMKIAILAALNIADEFSRFRKERDNAVGIWIEKTEEMSERLGKIIT"
     gene            6929..7143
                     /gene="ssrS"
                     /locus_tag="VNN25_01820"
     ncRNA           6929..7143
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VNN25_01820"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7197..>8502
                     /gene="rny"
                     /locus_tag="VNN25_01825"
     CDS             7197..>8502
                     /gene="rny"
                     /locus_tag="VNN25_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXH90289.1"
                     /translation="MNINGYVIAVLIGVAGAVVLAALWAAVYRKRGAAQIDEARRNAD
                     RILEDARKAAESKSKEADLEVKEKLLQMRSDFDRKSQQRNDELKTSEKRLQQKEENLD
                     KKTQQLDSRMAEVEKRDRAIDGREKTLEQREGELGELIEAQRHKLEQVAGLTMDEAKR
                     ELIRGIENEAKLDAAQIIKRIETEANEHGNLKARKILGMAIQRMASDYVAENTVSVVD
                     LPSEDMKGRIIGREGRNIRALELATGVDLIVDDTPEAVILSGFDPIRREIARISLERL
                     MQDGRIHPARIEELVEKVRQELDQKLFEEGEAAAFSLGITDIHPEVLRLLGRLRYRTS
                     YGQNVLMHSLEVAQLAALMAVELGVNDTIAKRGGLLHDIGKSIDREMEGTHLELGRQV
                     LEKYGEKREIIHAMECHHGDYDPTTVEAVLVNAADALSAARPG"
BASE COUNT         1590 a         2531 c         2794 g         1587 t
ORIGIN      
        1 gggggcttcg acttccccgg tgtcgcggca tcgttgacga cactgctgcc gtcctcgatc
       61 acgagccgga cgatcgattt ctcgaagacc tcttcggcgc acgcggacgt cgtcaatggc
      121 atgaacaacg gttcgctgct caccacctac gtcggccacg cgtcggtcga gatgtgggcc
      181 aacagcgtct ttctttcgtc ggatgcgagc gtgttgacca atggcacccg cttgccggtt
      241 gtctttgcga tgaactgcct gaacggctac tttcacgatc tctacaccga gagcatggcc
      301 gaggcgctgt tgagggcgcc taatggggga gccgtcgcgg tgtgggcatc ttcaacgctc
      361 acagagcccg accagcagtc gctcatgagt catgagttgt tccggcaact tttccgcacg
      421 gatgtgaacc tgacgctcgg cgatgctgtc acgcgcgcga agtttgctgc cactgaccct
      481 gacgttcgca agtcgtggat tctgttcggc gatccgacca tgaaactgcg gccatagctc
      541 accctttcaa tggaagtcgt ggctggccac gtcgccgatt ccttccagcg accagaactt
      601 gtccgcgcgc accgagcact gcccatcctc gttctgcagg atgccttcga cgattagtcc
      661 cggcgagccg acgatcagcg cgcgatgatc gcggaagaga tcaggcttca cgatggcttg
      721 tgagatgccg gtttcatctt cgagcgtcag gaagacgaat ccctttgcgg ttcccggacg
      781 ctgacggacg atgaccgcgc cggcagtggc gacgcgcttt ccattcggca gtttcttcag
      841 ttgcgtggcc gagtagacgc catcgcgttt cagttgcgga cggaagtgcg acagcaggtg
      901 agggccggtc gtcagatcca tcgccgcgta gtcggcgaga gtctcctcgc ccggcgccat
      961 ctcatcgaga ggagattccg ttgaggatgg tgcgatgctt tcgaatagct cacccgccgc
     1021 tttcgctgcc tgtgccgcct gccagagtga tgcgcggcgt ttgaggccga gggaggcgag
     1081 ggcgccagcg aaggcgagct tggtgagctg atcgtcgcgc agcatcgccc ggcgcgcgag
     1141 atcgtcggcg tttctaaatg gggaagacgc ttcgatcgat cgcgcggcgg tctctctcaa
     1201 ccccttcacg tacttcatgc cgaggcggac cgcgccgcgt tcccagcggc agtgccagcc
     1261 ggagtggttc acgtcgatcg gcagcaccgt cactccgtgg cgctctgcgt ctttgatcag
     1321 cgtcgcggga tgataaaagc ccatcggcca cgcgttcatc agcgcgatgt agaaagcggc
     1381 aggatgatgc gctttcaggt acgcgctggc gtaagcgatc agcgcgaagc tggcggcgtg
     1441 cgattcgggg aagccgtaca acgcaaacga ggtgatcgac ttcacgatct gatcctgcgc
     1501 cggcagatcg atgccattct tcgtcatgcc cgagcgcagc cgcttctcga tctcgcccat
     1561 ccgctccatc gatcgtttga agcccatggc gcggcgcagt tcctccgcct cgccgccggt
     1621 gaagtcggcg gccaccatgg cgatgcggag aagttgctcc tggaacagcg gcacgccgag
     1681 ggttcggcgg aggatcggct cgagacacgg gtgcggatat tcgagcggcg ctttgccctg
     1741 acggcggtcg aagaacggtt tgaccatgcc gccgacgatc ggtcccggac ggatgatcgc
     1801 cacctgcacg acgatgtcgt agaacttcgc tggtgcattg cgcggcagcg acgccatctg
     1861 cgcgcggctc tcgacctgga acatgcccac cgtatcggct tcgttgagca ttttgtagac
     1921 gcgcttgtcg tcctgcggca gatgcgcgag gtcgacgtcg atgccttcgt tcgtgcggat
     1981 gagcgggatc atttcttcga tcgctgcgag catgccgagg ccgaggaggt cgactttgat
     2041 gatgccgagg tcggcgcagt cgtctttgtc ccactgaatg acgacgcggc cgggcatggc
     2101 tgccggttcg agcggcacga cttcatcgag ccggccgcgg gccatcacca tgccaccgga
     2161 gtgttgcccg aggtgacgcg gcagattctg gatctgcagt tgaaggcgca tgaagtgctg
     2221 cacgcgcaga tcggccggat cgaagcctgc tccggccaac tcctgcggca tcgactccat
     2281 ccgctcgcgg atctcgccga agttccaggt gccgagggtc ttggagagtt tgtcggcttg
     2341 ttccagcgag aagccgagcg ccttaccgac ctcgcgcgcc gaggagcggt cgcgataggt
     2401 gatgacgttg gcggtcatgg ccgagccgta cacgccgtag gtttggtaaa cgtgctggat
     2461 gacgcgctcg cgttgatcgc ccgacggcaa gtccagatca atatccggcc actcgccgcg
     2521 ctcctcggat agaaaacgct cgaagagcag ttccatcgaa acaggatcga cggcggtgat
     2581 gccgagcgca taacagaccg cgctgttggc cgccgagccg cgtccctgca cgagaatctt
     2641 ctcgcgctgg cagaactgca cgatgtccca gacgatgagg aagtagccgg ccaggtcgag
     2701 cttctcgatc gtggccagct ccttttcgat ctgcgcctgc gctttggcgg tgagcgggcg
     2761 aaagcgcgtg gtggcgccgt tccatgcgat cttgcgaagg aacgacatcg gcgactcgcc
     2821 gtcgggcacc gggtactcgg ggaattggta gccgaggttg gcgagggtga agtcgagcgt
     2881 attggcgagt ttccaggcgc catcaagcgc ctcggggaga tcggcgaaga gcatggccat
     2941 ctcatccgca cctttgatga atcgctcgcg gttcacgccg aggagccggc cggcgctgtc
     3001 gacatgcttt ccttcgcgga tgcaggtgag gacgtcgtgc agctctttgt cctcagcgcg
     3061 tgcataacga acaccattcg tggcgatgag cgggagatga aagcggcggg cgaaatcgac
     3121 gagggcgatg ttgcgatgct cttcgtcgcg ccggtgatgg cgctgcaact cgatgtgcgt
     3181 gcgtcctttg aagatgccac tgatcttgtg cagcaatttt tctgcgtcgt cgacgccatt
     3241 cttcgtcaac gcgtgcgcga gcggaccctc atcgccgccg gtgatgcaat gaagaccctc
     3301 ggcgtattgc gcgatgaggt cccacgtgaa tcgcgcctgc gctttgggac ggtcgagcgc
     3361 gccggcagtg aggagcttgc agagattgcg gtagccggta tggctttcga cgagaagcgt
     3421 gagccgcgtt tcgcgcgcat tcgagcgcgc ggcgtccatg cgcgtgaggg tcagtccggg
     3481 cttttgctgc tcgttcccaa gcacgacctc ggcgccgacg atcgcgcgca ctcccgcttt
     3541 cttcgccgca ccataaaagc gcggtgcacc gtagacgcca ttcgtgtcga tgagcgccat
     3601 tgcagggatg tctttcctgg ccgcgtgctc gatcaagtct tccggaagac ttgcactgtc
     3661 aaggaaagag aacgcgcttg ccgatcgcag ctcggcgtat gggcgtgatt ttttttgatc
     3721 cgcagatgac gcagatgaac gcagatgtcg attgccttta tctgtgtgca tctgcgtcat
     3781 ctgcggatca atccctacat ttcataccgc gcatcaacaa accaatcctc ggacacaccg
     3841 cggaagagtc gatacacacc gccgcgctcg agctcgacgt cccagtattc gcggacatcg
     3901 ggccggtcgc tccaccatcc ttcctccaca cgccatgggc cggagcagat gcgcaccgtg
     3961 ccggcgatct cgatcgtggc gccgacttcg atgcgtttcg tggagtgggg agtggagggt
     4021 gggagggagt tggcgatggt gcggattgag gcgatctggg tttctttatc cgccgtctcg
     4081 cgcgtcgtca cttcgatggc gatgggaggg cgaaatgtgc ggacagcgag cagcccgtgc
     4141 gcacgcgacg gtttgcgccg gacatcaggt ggtgggggag ggtcgaaatc gctgatggcg
     4201 aaacgttcgg ggagatggcc gtcgaccgtc atcggcatgc caacgcggcc atcgccgagg
     4261 agtgcgacga gcttggcgat cgtggtggcc aacttctccg gcgacatcgc agtcggacca
     4321 aacagcgaga gctgcgcgcg gcggggcttg tcaggatggg cgacgaagga gaagccgacg
     4381 accggcgcgc cgggcggggt cttctccaga tcgaggcgca tcagtgtcag catcgtcttc
     4441 acgtcgcgtg tcggtgcggg gagatcgatg ccgcgcgcgt cgtagccatc aggatcgagc
     4501 gtcagcgtga attcgaggcg cttgcaggcg aagccctgga tctccatccg tttggccagg
     4561 cgatcgagcg cggcgttggc gatgaagaga aacggctcga gggcgacgag cggccattcc
     4621 agctccatcc cttcgcggaa ttcaggcggc atcgcgcgag gcatgagagg acgcggatcg
     4681 atgccgcgcg cggcccagtg aagctcgcgc ccgatttcgc cgagccggct ggcaatctcg
     4741 ctttccggga gccgggccag atcgccgatc gatgagagcc cgaaacgctg cagcattgcc
     4801 gccgcttcaa gctccggcga aagacgcgtg agtggaagcg gagcgagaaa cgtcctttct
     4861 tctcccggtg caatgacatt cggcgacttc ggcaactccg ccgcaacccg cgccgccagc
     4921 ttgctcgccg caatgccgac gcgcgccggc agcgagacgg cttcgcacgc gcgaatggcg
     4981 gcgcgggcga ggaggtgttc gggggtacgt aggtcggact ctctagtccg actgctcgag
     5041 atgtcggact ggagagtccg acctacgtca ctcatggcat caacaaagtg cctctccatc
     5101 cccatcacat cgagaaagac gaccccttca ccggcgtctt cgacgcgcgg cgagaaggat
     5161 tcagcgactt cgagcagcgc ttcctgcgcg gtgcgctcgc attcggcgtc acggccgcgg
     5221 gcgatcagct ttgggaggag cgagcgagcc tgcgtgagcg agagtcccgg atggatgcct
     5281 ttctgacgtg cccttctcgt ggcggcgacg atgtgtgcgt tgttgccgtt cccttcaacg
     5341 atgccgaccg cttcgtggag cagatcgggc tcactgcgca gccgcgccgc cagcgcaaac
     5401 atcgggacgt agaaacaggc aaggcgagtc atgaggcttt gcgggtgtcg caggtcggga
     5461 ggcgggagtc ggagaaagct ttgagcggat cgcgaccttg aagttgtgaa gcggagggcg
     5521 aggatgtatt cgggttttgt atccgactcc cgacccccga ctcacgaccc ccgcgtatcg
     5581 cctcctcaca ccgaaagaag accgagacgc tctgccctgg ccgcatgcga cgatgcttct
     5641 ccagtttcag ccgcgtctcg attccagcca gaacgcgcgg cgcattccct cggcccagcc
     5701 atcgcgcgtg cgctgcgtac gcgaggatca ttgcttccga ggttgtgccg gtgagggcgt
     5761 agggggcgga gacgaggatc gtcgcgccgt gcgattcggc gacgcgggcc aggcggaccc
     5821 aggcggcgtc gggcacgcgc cggccggggg gacgtcgcgt cgccatatca acgatgacga
     5881 gctgaaagcc ggtggcgccg agcatctccg cggccgtcac catctgtttc atcgtgcgcg
     5941 gacgagccca aaggagtcgc tgcagatcga cgccggccgc ttccgcgagt tgcggatcga
     6001 agcaatcgcc ggcatcgatg agggctgctg cttcgccgat gctggtggcg gcggcaagcg
     6061 cggcaatgac gatcgagaag cgcgttccgc gtccggcgat ctccgtcatc ttgccgcgcg
     6121 acaatccgcc gccgaggagc gcatcgagcg gctcgatggt cgtgggaagg atctcttcgc
     6181 gtcgctgacg agccagagcg cgaatcagtt ccgagccgct ggtcagcttc gacgcgatcg
     6241 atgcctcgag gctcgcgcgc aggttgcgga tggcgtcatg ttcgccgggc gggatgggag
     6301 gtgggtcggg gagggggcgg agggccgact ccaccactcg tggagtcctt gccggggttg
     6361 agatcgaggt ttgaaggagc tgaaactgcg agtcgagagt acgagcgtgt acggccatgg
     6421 gggaagtcgc atgactaact taggcggtaa ttcagcgtaa gtcaatgaac agttccttga
     6481 tccttcgcgt cttcgcggct tcgcgtgagc aggtgtcacg cgaagccgcg aagaacgcga
     6541 aggagaatcg cacgcgcatc cgtctgctgc tataatcctc gcttgttcta aggtaaattt
     6601 ttccgatgaa cggtgaatac gactcgacca ctacggtcga gatctttggt cagacgtaca
     6661 acgttcgtgg ggagggagac ccgaactatt taacggagct tgcgcggttc gtcgattcca
     6721 ggatgcgcga ggtcgcagcg caggtcgcta cggtcgatcc catgaaaatc gccatcctgg
     6781 cggctctcaa tatcgccgat gagttttccc gattccggaa agagcgcgac aacgcggtcg
     6841 gtatctggat cgagaaaaca gaggaaatgt cggagcggct cgggaagatc atcacctgag
     6901 cctcatgttt tcagacaggc agttttcgga gtccctgcag agttcgtgat gaacgatcta
     6961 actcttgaac cgatgctttt ttcttacttc gggtgctcgc gtcttcgggt cgcgtgcacg
     7021 ccgtccttcc gagtggcaac attcggaata tggagacggt agcctaaacg gcacgggtag
     7081 agcgcccaac ctgtttctgc aggttcaagt aagaatccaa cacacggctc aggcggggtc
     7141 ctccgaaatt tcctgacgac agaagcgcgc aagcctcccg gaacagggga ggcagtttga
     7201 atatcaatgg atatgtgatt gcagtgctca tcggcgttgc gggcgcagtc gtgctcgccg
     7261 ctctctgggc agccgtttac cgtaagcgcg gcgccgcgca gatcgacgaa gcacgccgga
     7321 acgctgaccg catcctcgag gatgcccgca aggccgccga gtcgaagagc aaggaagccg
     7381 acctcgaggt caaggaaaaa cttctgcaga tgcggtcgga cttcgaccgc aagtcgcagc
     7441 agcgcaacga cgagctgaaa accagcgaga agcgcctgca gcagaaggaa gagaacctcg
     7501 acaagaaaac gcagcagctc gattcgcgca tggccgaggt cgagaagcgc gaccgcgcca
     7561 tcgatggccg cgagaaaact ctcgaacaac gcgaaggaga gctcggcgag ttgatcgagg
     7621 cgcaacgcca caagctcgaa caggttgcgg gtctgaccat ggatgaggcc aagcgcgagc
     7681 tgatccgagg catcgagaat gaggccaagc tcgacgcggc ccagatcatc aagcgcatcg
     7741 agaccgaggc aaacgagcac ggtaacctca aggcccgcaa gatcctcggc atggccatcc
     7801 agcgcatggc ctccgactac gtcgccgaga acaccgtctc cgtcgtcgat ctcccaagcg
     7861 aagacatgaa gggccgcatc atcggccgcg aagggcgcaa tatccgcgcg ctcgagctgg
     7921 ccaccggcgt cgatctgatc gtcgacgaca cgccggaagc ggtcatcctg tccgggttcg
     7981 atccgattcg ccgcgagatc gcgcgcatct cgctcgaacg cctcatgcag gacggccgca
     8041 tccatccggc ccgcatcgag gagttggtcg agaaggttcg ccaggagctc gatcagaagc
     8101 tgttcgagga aggggaggcc gcggccttct cgctcggcat caccgatatc catcccgagg
     8161 ttctccggct cctcggcagg cttcgttatc gcacctcgta cggacagaac gtgctgatgc
     8221 actcgctcga agtggcgcag ttggccgcac tcatggccgt cgagcttggc gtcaatgaca
     8281 cgatcgcaaa acgcggcgga ttgctgcacg acatcggcaa gtcgatcgac cgcgagatgg
     8341 aagggacgca tctcgagctg ggacgccagg tgctggagaa gtacggcgaa aagcgcgaga
     8401 tcatccacgc gatggagtgt caccacggcg attacgaccc gacaacggtc gaggcagtgc
     8461 tcgtgaacgc cgctgacgca ttgtccgcgg cgcgtccggg cg
//
LOCUS       DATLVW010000054         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_127129, whole genome shotgun sequence.
ACCESSION   DATLVW010000054 DATLVW010000000
VERSION     DATLVW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127129"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VNN25_01830"
     CDS             complement(<1..242)
                     /locus_tag="VNN25_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90290.1"
                     /translation="MRAPLAILLLFAATSVSAAIVDDFDDVAKWTAAPSDGVSLNLVS
                     DHGAMRMDFDFRGHGGWAAARRHFDIALPDNYRFAFR"
     gene            381..1619
                     /locus_tag="VNN25_01835"
     CDS             381..1619
                     /locus_tag="VNN25_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH90291.1"
                     /translation="MRRRTKIIIGVVALVIVGVVTAGFVSQRGKDVTPVTFGKVQRQD
                     LTSKVSANGRIDAKRKVDLSANVMGQVINLAVREGDVVKKDDFLLQIDQKQLAATARS
                     ADAALQAVFSDRDAAKAMAIAADQDFQRAQKNYDQKIIPRADLDRAQSSRDSARANVM
                     ALEQRISQARENLNAARDTLSKTTMRAPMAGIVTALPVEEGEVAVIGTMNNAGTVLMT
                     IADMSVVEAVMEVDETDVPSVKVGQRASVTIDAYPNKTFPGLVTEVASSPIKRNGVTA
                     SGSTEAVNFEVKIQVENPPPGVRPGFSASADIITGTRSKALAIPIQALVVREKPGSTP
                     GKPQDEEGVFVHQNGTVKFETVKTGLSGDSMIELVSGAKEGQQIVTGPFRALREIKDG
                     TKVKEQKEKEGEGTSGDKKS"
     gene            1717..>2621
                     /locus_tag="VNN25_01840"
     CDS             1717..>2621
                     /locus_tag="VNN25_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH90292.1"
                     /translation="MDTLRIAVAALAAHKLRSFLTLLGVIIGVMTVISVVSIISGLNA
                     YIAEKVFTLSPDVYIVTQFGIITSREQFLIAVKRKKIDWHDYEAIRQQCTRCAAVGAQ
                     ENTRMTVKNGSQKLGGVRIQGGTANIGEINNVDIEEGRFFTRTEDEHAAMVAVIGSDV
                     KDELFGKLDPLGRTIHIAGHPARVIGLLRKQGSVLGRSQDSQLYMPLSAYQKAFGTRG
                     GLNLFVRAAGGVPGLEKSLDQVRVVMRSRRKTGFRSDDPFGVVSAEALQTLWKSITAA
                     GFAVMIFISGISLVVGGIVITNIMLV"
BASE COUNT          548 a          795 c          853 g          425 t
ORIGIN      
        1 cggaaggcga agcgatagtt gtccggcagc gcgatatcga aatgccgccg tgccgccgcc
       61 cacccgccgt gaccgcggaa gtcgaaatcc atccgcatcg cgccgtgatc ggaaaccagg
      121 ttgagcgaca cgccgtcgga gggagccgca gtccatttcg cgacgtcgtc gaagtcgtcg
      181 acgatcgctg cgctgaccga tgtggcggcg aagaggagaa gaatggcgag cggcgcgcgc
      241 atgagtaccg ctagtttacg gttcgttgag taaacgcggg gcaacccggt cgccgccggg
      301 gcggcgattc cttcaatcgc ccggcgcgcc gcaactccgc gactgcgtta ccgtatagct
      361 cttccaccaa ggagatcttc atgcggcgtc gtaccaagat catcatcgga gttgtcgcgc
      421 tggtcatcgt cggcgtggtc acggccggat ttgtgtcgca gcgcggcaag gacgtcacgc
      481 ccgtcacgtt cggcaaagtg cagcggcaag atctgaccag caaggtttcg gcgaacgggc
      541 ggatcgatgc gaaacgcaaa gtcgatctct ccgcgaacgt gatgggtcag gtcatcaacc
      601 tcgccgtgcg cgagggcgac gtcgtgaaga aggacgactt cctcctgcag atcgatcaga
      661 agcagctcgc cgcgacggct cgaagcgccg atgccgcgct gcaagccgtt ttctcggacc
      721 gcgacgccgc gaaggcgatg gccatcgccg cggatcagga cttccagcgc gcccagaaga
      781 actacgatca gaagatcatt ccgcgcgccg atctcgatcg tgcacaaagc tcgcgtgatt
      841 ccgcgcgcgc caatgtgatg gcgctcgagc agcgcatcag ccaggcgcgc gagaacctca
      901 atgcggcgcg cgacacgctc tccaaaacca ccatgcgcgc gccgatggcc ggcatcgtca
      961 ccgcacttcc tgtcgaagag ggcgaagtgg ccgtgatcgg cacgatgaac aacgccggca
     1021 cggtgctcat gacgatcgcc gacatgagcg tcgtcgaagc ggtgatggag gtcgatgaga
     1081 cggacgtgcc gtccgtgaaa gtcggccagc gcgccagcgt caccatcgac gcctatccga
     1141 acaagacgtt cccgggcctg gtcaccgagg tggcgtcgag ccccattaaa cgcaatggcg
     1201 tcaccgcgag cggcagcacc gaagccgtga acttcgaggt caagattcag gtggagaatc
     1261 cgccgccggg tgtgcggccg ggattctcag cgtcagccga catcatcacc ggcacgcggt
     1321 cgaaagcgct ggcgatcccg attcaggcgc tcgtcgtgcg cgagaaaccg ggcagtacgc
     1381 ccggcaagcc gcaggacgag gaaggagtct tcgtccacca gaacgggacg gtgaagttcg
     1441 agacggtgaa gacgggtctt tccggcgaca gcatgatcga gctcgtgagc ggcgcgaaag
     1501 aagggcagca gatcgtgacc ggcccgttcc gcgcgttgcg ggagatcaag gatgggacga
     1561 aagtgaagga gcagaaagag aaggaagggg aagggacgag cggagacaag aagagctagt
     1621 tgcgggggtc gcggttcggg agtcgggagt cggagagaaa actcagtctc cgctccgacc
     1681 cccgaccccc gaccagcgac ccccgacacc aaaaccatgg acaccctccg catcgctgtc
     1741 gccgcccttg ccgcgcacaa gcttcgcagc ttcctgacgc tgcttggcgt gatcattggc
     1801 gtcatgacgg tgatctcggt tgtctcgatc atctccggac tcaacgcgta catcgctgag
     1861 aaagtgttca cgctcagccc ggacgtttac atcgtcacgc aattcgggat catcaccagc
     1921 cgcgagcaat tcctcatcgc ggtgaagcgc aagaagatcg actggcacga ctacgaggcc
     1981 atccgccagc agtgcacgcg ctgcgcggcg gtgggggcgc aggaaaacac gcgcatgacc
     2041 gtcaagaacg gctcgcaaaa actcggcggc gttcggatcc agggcgggac tgcaaacatc
     2101 ggagagatca acaacgtcga tatcgaagag ggacggttct ttacgcgaac cgaggatgag
     2161 catgccgcga tggtggccgt gatcgggtcg gacgtgaagg atgagctgtt cggcaagctc
     2221 gatcccctcg gacgcacgat tcacatcgcc ggccatccgg cgcgcgtgat cggactcttg
     2281 cgaaagcagg gctcggtcct cgggcgaagt caggacagcc agctctacat gccgctttcg
     2341 gcctaccaga aggcctttgg tacgagaggc ggcttgaatc tctttgtgcg ggcggcgggc
     2401 ggagttccag gactcgagaa atcactcgat caagtgcgcg ttgtgatgcg cagccggcgc
     2461 aagaccgggt ttcgctcgga cgatccgttc ggcgtcgttt ccgccgaggc actgcagaca
     2521 ctgtggaaga gcatcactgc cgccggcttc gctgtgatga tcttcatctc cggcatctcg
     2581 ctcgtcgtcg gcggcatcgt catcacgaac atcatgctgg t
//
LOCUS       DATLVW010000055         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_334233, whole genome shotgun sequence.
ACCESSION   DATLVW010000055 DATLVW010000000
VERSION     DATLVW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334233"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VNN25_01845"
     CDS             complement(<1..1092)
                     /locus_tag="VNN25_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HXH90293.1"
                     /translation="MPAPLCFVLMPFGDKTDAAGKIIKFDEVYKQVIQPAISDAGMMP
                     IRADEELTRGMIHKPMFERLILCDFAVADLTTANANVFYELGVRHAVRPGATVLLFAA
                     GGRLPFDAAPLRSVPYDLSDTGVPADAATARAELTKQLKHIREARDDDSPIFDLLKPF
                     PSAIDHLKTNTFQDQVALTQQMEEQLRSAKTADELKTFEANLGALDGVESAILVKLFL
                     SYRDAKAWQSMIDLAAAMPEPLRRTVMIREQLGLALNRAGKSDDAEHVLTTLIQERGG
                     SSETYGILGRVYKDRWQKATNNFIAKGELNRAIDAYLKGFESDWRDAFPGVNAVTLME
                     VRNPPDPRRLDILPIVRYAASRRVSAGSPD"
     gene            1381..>2034
                     /locus_tag="VNN25_01850"
     CDS             1381..>2034
                     /locus_tag="VNN25_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH90294.1"
                     /translation="MTTGRARHTGLRAAVDHLFGYDVFISYSRGDGAAFGRGLATELE
                     EMDLSCFLDENELPAGYPLSGTLRAALRRSSVLALVSTKAARASTFVAMEVSTFAATK
                     RPIVPIVIREAEGDIPEEQPEIPWESIRDRELVWVSDVADAVSASTVTAVRDALRVTR
                     KRTLASRVMFSAAAVLLLVSLLAGGAAVIAIREQQRAEQEAKSARQAAARARAEEKRA
                     "
BASE COUNT          357 a          605 c          677 g          395 t
ORIGIN      
        1 gtccgggctt cccgcggaga cgcggcgcga cgcggcgtaa cgcacgatgg gcagaatgtc
       61 gagccgtcgc gggtcaggcg gattgcgcac ctccatcaat gtcacagcgt tgacgccggg
      121 aaacgcgtcg cgccagtcgg attcgaagcc tttgaggtag gcgtcgatgg cgcgattgag
      181 ctcgcccttg gcaatgaagt tgttcgtggc cttctgccac cgatccttgt agacacggcc
      241 caggatcccg taggtctcac tgctgccgcc gcgttcctgg atcagcgtcg tcagcacgtg
      301 ctccgcgtcg tcgctcttcc cggcgcggtt cagggcaagg ccgagttgct cgcggatcat
      361 gaccgtgcgc cgtagcggtt ccggcatggc ggcggcgaga tcgatcatgg actgccaggc
      421 cttggcgtcg cgataggaaa ggaacaactt gacgaggatc gcggactcga ctccgtcgag
      481 cgcgccgaga ttcgcttcga acgtcttcag ctcatcggct gttttggcgc tgcgcagctg
      541 ctcctccatc tgctgcgtga gcgcgacttg atcctgaaat gtattcgttt tcaggtggtc
      601 gatggcggag gggaaaggct tgagcaagtc gaagattgga ctgtcgtcgt cgcgtgcctc
      661 acggatgtgc ttcagttgtt tcgtcagctc ggcgcgcgcc gtcgctgcat ccgcagggac
      721 gcccgtatcg gagagatcgt acgggaccga gcgcagcggc gccgcgtcga acggcaatct
      781 gcctccggcc gcgaaaagca gcacggtcgc tcccggccgc accgcgtgcc ggacgccgag
      841 ctcgtagaac acgttggcgt tcgcggtggt gagatccgcg accgcgaagt cgcagaggat
      901 caaccgctcg aacatcggct tgtggatcat gcctcgcgtc agctcctcgt cggcgcggat
      961 cggcatcatc ccggcgtcgg agatcgccgg ctggatgacc tgcttgtaca cctcatcgaa
     1021 cttgatgatc ttgccggcgg catcggtctt gtcgccgaag ggcatgagaa cgaaacagag
     1081 cggtgctggc atggtggtta gcatagcagc gtgaggtttt cagggggctc ttccccgtca
     1141 acggccctgt cggaagaacc gcccccttct cctccgtgac gggcgggagg tcgctgtatt
     1201 ttcacaagca ataggctgtc ggataagttg tatgaattga gcgagttggg ctccgcaata
     1261 cgacgtacga acttgccgcg cctcgcaagg tgcagacgtc gtgtgagtgt gacgaactgc
     1321 caaggctacg cccgtggaag ctcagcggaa acgcgggaga tgtgcgctat tgtatttgct
     1381 atgaccaccg gtcgcgcacg gcacaccggg cttcgcgccg cggttgacca tctattcgga
     1441 tacgacgtgt tcatctccta cagccgcggt gatggcgcag cgttcggccg cggtctcgca
     1501 acggagctcg aagagatgga cctctcgtgc tttctcgatg agaacgagtt gccggccgga
     1561 tacccgctca gcggaacgct gcgcgccgcg ttgcgccgaa gcagcgtgct cgctctcgtc
     1621 agcaccaaag ccgcgcgcgc ttcgacgttc gtggcgatgg aggtgagtac attcgccgcc
     1681 acgaagcgtc cgattgttcc gatcgtgatt cgcgaagctg aaggcgacat tcccgaagag
     1741 cagcccgaaa ttccgtggga atcgattcgc gaccgcgagc tggtctgggt gagtgatgtt
     1801 gcggacgccg tgtcggcttc gacggtgacg gcggtacggg atgcgctgcg tgtgacacga
     1861 aagcgaacgc ttgcatcccg cgtcatgttc agtgcggcgg ccgtactgct gctggtctcg
     1921 ctgcttgccg ggggtgccgc cgtcattgcg atacgcgaac agcaacgcgc cgagcaggaa
     1981 gcgaaatctg cacgccaagc ggccgcacga gcacgtgccg aagagaagcg cgca
//
LOCUS       DATLVW010000056        16042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_457162, whole genome shotgun sequence.
ACCESSION   DATLVW010000056 DATLVW010000000
VERSION     DATLVW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16042
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457162"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1129
                     /locus_tag="VNN25_01855"
     CDS             134..1129
                     /locus_tag="VNN25_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90295.1"
                     /translation="MERVRTAQPLRLFSVGAIAYTAIAILLVRSQVYATHPDVLAWGL
                     TFDLGISIPLIYWFLFVRTGVMRPMTLIPLFVIAVGIASRIVPPGQHHFAQQLGYVAA
                     PLDLVTLWMVARRLMRARSAAAEDSDVPTRIAQITRQLFGDGAVAGFVALEISSIYYG
                     LFCWRKRAAPGFSIYKRSGWDTVLICILVLITAESIGVHLLLQAWSVRTAWVVTLLDL
                     YGAIWLLGDYHAMRLRPITIDEQSLHVRFGFRWSISIPLNTIASIEPIRSESDWKRKG
                     VLKVALLDEPRLLIRLDAPAIATGLAGIRKTIDAVAVLPDDLEGFEEALRILIRR"
     gene            complement(1202..1645)
                     /locus_tag="VNN25_01860"
     CDS             complement(1202..1645)
                     /locus_tag="VNN25_01860"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HXH90296.1"
                     /translation="MADATQSFSNHRRFLPLFHFVALPLLIINLIIRIVYAIMHRGAR
                     LVWWEIAFALALVLLALTTRQMVLTVQDRLIRLEETLRLQRCLPEDLRGRIGELSSSQ
                     LIGLRFCGDDSELAALVRSAVNGELKGREDIKKQIKTWRPDTMRA"
     gene            complement(1680..3479)
                     /locus_tag="VNN25_01865"
     CDS             complement(1680..3479)
                     /locus_tag="VNN25_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90297.1"
                     /translation="MIRNALLLALTAALLLPLPAQATVGCCNFCPDQYEQEGPYPYFE
                     VIRTQRQLLDQDVVAGAGWKRIFESCSQVNRVNVTASQDATVVVRAFLQLNNQTTPGV
                     DVEYRWLLDDVPVGAVFHFRVGLAGFPHGDDINIVLPNVAAGAHRVAIDARVLAESGT
                     IKFRLMFVTAQGFPSQHFPGASLISDRAVTIGTDWTMVGTKFAVAPAEEARLYLQSYV
                     AADAKSTIDFRYVVDGFPMEPFTVVMPEGGGLALWDHFADILDPGSHPVHLEARGDSA
                     ATISMTQVEAATAPATYSSYLLPWSDIDAVGSLGDAHQPLNCLILSAAGAGGTGGLGG
                     TPACGKYQLLLDSQLDAAGASDDYAIFGDGYVEIENRSATSGIVTLTVEAIYEDASAA
                     FCNTLIDTPNSLCANPLQCSTADFTISEFTVLPGRTQKFFYVDPVHWGSSKPNHIRLW
                     ARSQDGCGAAPVDLVFGHSRLGLQLVPAGVGSCFSARAQGRAAAPALTVVASAGKAVL
                     QWQSPESINGSWEILRATDQGSFSLIARNPSGLRTLTDTTVKPGVAYRYEVRAYAVST
                     DFAVGSSYVCGGLSNEIRVVVPPVAPRKRASGH"
     gene            complement(3479..7384)
                     /gene="dnaE"
                     /locus_tag="VNN25_01870"
     CDS             complement(3479..7384)
                     /gene="dnaE"
                     /locus_tag="VNN25_01870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXH90298.1"
                     /translation="MSFAHLHLHTEYSLLDGASRPEELAKRVAKMGMPACAITDHGNM
                     FGAVEFYNAMKKEKVKPIIGCEMYVAYGSRFDKAGVEDGQADAGANNHLIVLAANNEG
                     YKNLVKLVSAGYTEGFYYKPRIDKELLREHREGLIVLSSCLKGEVSQSLAGGNLTKAK
                     EAALQYKDILGADNFFLEIQDHGIPDQQKIVPMMARLGEETGLRLVATNDSHYLSKDD
                     AFAHEVLLCIGTGKTLGDEKRMKFYSDDFYVKSPDEMRRIFSGYGEALDNTVKIAERI
                     NVTLDVKGYHLPNFPVPEGKDLAGYFAEVARAGLERRLKAAAPLFAAKKKKHEVQAYH
                     DRLESEIDIIRTMGFPGYFLVVWDFIKYAKDNGIPVGPGRGSGAGSLVAYSMGITDVD
                     PLDYDLLFERFLNPERLSMPDIDIDFCMNRRGEVIEYVRRKYGADKVAQIITFGTMAA
                     KSVVRDVGRVLGLPYGLVDKVAKTIPTGPDVTLPTAAKDSPALIEAMKNDHEVERIIE
                     IGSRLEGLSRHAGMHAAGVVITPEPVTNYVPLYRTNRDEIVTQYDMRIVEKMGLLKMD
                     FLGLRTLTVIDDAIKSAKAVEGVTIDIETLPLDDPEVFRLFQEGRTKGVFQFESGGMV
                     DLLRKSRPTRFEDLAALNALYRPGALDAGMVDEFVRRKNGLSKAKYLVPAMKGILEET
                     YGVIVYQEQVMQIAQQVAGYSLGQADLLRKAMGKKDAAVMAAERDKFVTGAVAKGIDK
                     KKANDLFDYIEPFARYGFNKSHSVAYALVAWQTAWLKVHFPRHFMAALMTSEMDRTDF
                     VVKFINEASQMGIRILPPDINESNYAFTVVGPNIRFGLGAVKGVGGSAIESILEARHR
                     KGRFASLLDFCEAVDLRACNKKVIEALIKSGSFDFLGTPRKVLFETLENTADSAQRAR
                     EEKERGQSSLFGGMMAAPAPPPLPSAQAAALHKLAEWSDDEKLRFEKETLGFYITGHP
                     LNKFADELSIFANATTDTLHQHIDEIVNIGGIVSQIKKSKIKKGPNEGKLMAKFILDD
                     QRGSVEVVVFSDLYSKYAKWLDNGIAVLLTAAVKDTGGMQAGRSAALQSAEQHAQRAD
                     DEYAHVSGYDVSEDDVEDDRDPKEIEAEKYGDRANNLGLFGGVGSGELGVAAESPILD
                     DEALDDSSSISDTRFTTPDDDDLSPTPDTPLPTPDETNFASHAATFHEAPITPELNAL
                     EIVPLDGIRDKKVKEIALEVAYPRMTEETVRKIREIVDEHPGEVPLTISIVELPQSLI
                     DNSPRGEVKLRVNQHFRVQPGASLTAKLLQVHATAKYVF"
     gene            complement(7492..9501)
                     /locus_tag="VNN25_01875"
     CDS             complement(7492..9501)
                     /locus_tag="VNN25_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXH90299.1"
                     /translation="MKLRVLAAIAVLCLVSAFEASGADLLNDLRTPSLSEPVAVNNVT
                     LTIGHLKLMMASGSAAKLSAAGEPVGFFFKGAGRFEYVAETTEMPVVTRNVKSDSHAT
                     LAGATISEDVTEAMVMLVGATSPDLGTSTAGTSLADDLKAHQAVFKFAQIEPGEHLIA
                     AQKLGLPGAAAYIEIVTPKDKLVYRYDSADLQEESLTSVHIWNSYVSDKTLSAYLFPT
                     ILSSQPVGRDVRAYARPAFTITALDYSLTTDGDNAKLEMTETIAPRFGGQRAFRFNLD
                     TELIVVANKPARRINVRSVVDEQGKPVSFAHEIDNLLVVLPAAATQPFKLKFVIDGDF
                     LFREEGDNAWQLVNFAWYPKSRNWAGNYSTVHSLIKVRKPFIPIAPGTLIARKEEGDY
                     NVVENVVEKPIAFPIVEGGKYHIFEEKRDDRTVRVATYGIASEKGAKTLIGMTFGFIE
                     YYEYFLGPFPWKEFNIIQVNTYGYGQAPDATMFITNEAFNPLKPSDEDYTMSAEQYFS
                     QGINERIAHEIAHQYWGSAVKMPSPEEQWLTETFAEYSAGLLLKKFKGDAVFNRMINS
                     WRARATQAAAIAPIPYANRIGGDRFMARAARTQLLYDKGPLLLAAIHKQLGDEKFLTF
                     LKSYTKSFQGKFGTTADVAGLLGFMTKQDWKPFFDQYYWGLAMPQ"
     gene            complement(9608..10237)
                     /locus_tag="VNN25_01880"
     CDS             complement(9608..10237)
                     /locus_tag="VNN25_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90300.1"
                     /translation="MATMLSLAVTTSPYLSQVQIVVWAVTIVVGGIGAYKGIRELQMS
                     TESRKTELRWKQAAVARDLLGEIHHGFYSSAAVQMFDWWDCTRNYSVKHDGIVLVSDA
                     GEKGLIEIATDDIRAALALPMHQQINEKEIFIRDCFDWFFYYVDRIDQYVLNGLVTIE
                     DVAAVFNHYSKLILRERELFEAFMKQHEYHYAVHFFRRHLPSYLPSPDS"
     gene            complement(10294..11019)
                     /locus_tag="VNN25_01885"
     CDS             complement(10294..11019)
                     /locus_tag="VNN25_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90301.1"
                     /translation="MPKFFFYAGTGFCLFLGLCAAAGLMSQAEGGPMVFAAVRGTFVS
                     GTKAAAWAIMVALIAGIATAAILIRMAALLAARNVFGALFALLATTASVVALAWTLLL
                     QARMVMLVRHGLGYAELAPLHFAALMMLGCFVSLAFLALRPYFSVQASRVLSALVFLP
                     FPLYCLILEQEFFVSTKLSPLPSVSPASNVFFGVVAILFFAVAMHCVRHRHMFIETTN
                     LRELLDTRVDRTARPIGGVAFGS"
     gene            complement(11254..11760)
                     /locus_tag="VNN25_01890"
     CDS             complement(11254..11760)
                     /locus_tag="VNN25_01890"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HXH90302.1"
                     /translation="MTRFEIVPGDITKLKVDAIVNAANTSLLGGGGVDGAIHRAAGPE
                     LLAACRLLNGCATGDAKITPGFRLPAKYVIHTVGPVWHGGTHDEPALLASCYRRSLEL
                     ALANDVRTIAFPAISCGVYGYPIDAAAEIAVGETSRVLAGNELIDQVLFVAFGDDVRR
                     ALESALRR"
     gene            complement(11757..12218)
                     /locus_tag="VNN25_01895"
     CDS             complement(11757..12218)
                     /locus_tag="VNN25_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXH90303.1"
                     /translation="MKYWLMKCEPSAYSIDDLARDGTSSWEGVRNYQARNFMRDEMKT
                     GDRVLFYASNADPSGVTGIAEISREAYPDKFARQKGHDYYDPKATEENPIWYMVDIRF
                     VEKFPHIVSLETLKSAPGLEEMVVTKKGSRLSIQPVTKSEFGIVRALGRSK"
     gene            12291..13046
                     /locus_tag="VNN25_01900"
     CDS             12291..13046
                     /locus_tag="VNN25_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90304.1"
                     /translation="MRLRIPPASASLILIAIFASRLAAAEPPCELNYRTDGTTSETSV
                     LTSVAPETVVERLPFLLAKAGVTIHWSQPPKGIIEAEGLDVKAGKSGDDTRVTFRSHL
                     QPPANSATLCRYASLAGSPPEPKRPPVAQDPALIERMKDDLLKLHTIVMQGSRLNNVQ
                     FSSPSNFLQFAVTDIKASPVKKEYKVSMLLPRSICAISHEDMDDLTLEMSGEGRSAPR
                     TKPVRVEMSLVYEGEGAASHLSEASILKIESTK"
     gene            13054..13761
                     /gene="truA"
                     /locus_tag="VNN25_01905"
     CDS             13054..13761
                     /gene="truA"
                     /locus_tag="VNN25_01905"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXH90305.1"
                     /translation="MRLLLTIQYLGTRYAGWQSQTNATAIQNVIEQALSTMFGGSIRI
                     EGAGRTDSGVHARAQRAHFDAPFDIEMRGFRRGLNDLLPRDIRVIGVESVADDFHCRF
                     ITKSKTYVYRIWNDEVADVFAAETSAHVPQPLDVALMHTTAQQLQGRHDFAIFTVAGP
                     EVSSTERTITAIDVVGNGAAIAITVSADGFLRSMVRRIAGSLIEIGRGKLEPEAIWTV
                     ARWTAPAKGLTLWEVRY"
     gene            complement(13949..14071)
                     /locus_tag="VNN25_01910"
     CDS             complement(13949..14071)
                     /locus_tag="VNN25_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90306.1"
                     /translation="MRSAVLLTQNVGCSFNEPSRFCVSGRIFLTQRFDRHFYMS"
     gene            14189..14410
                     /locus_tag="VNN25_01915"
     CDS             14189..14410
                     /locus_tag="VNN25_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90307.1"
                     /translation="MRQEIREESAAAHADTRLLFGATAEDMRRLVKNVADGVTLANEK
                     IDRFDEKIERIAAGLELRIIRLEVESSRR"
     gene            14815..15630
                     /locus_tag="VNN25_01920"
     CDS             14815..15630
                     /locus_tag="VNN25_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXH90308.1"
                     /translation="MIDLRKLAEPGSIEETLLDKIDFKRLPRHIAVIMDGNGRWARSR
                     NLPRVEGHRAGIAAVREIVETAARLELGVITLYAFSVENWKRPRFEVATLMMLLKEYL
                     RKELATLMDNNIRFTAIGRVEGLDPSVQRELKYAADQTARNSGLLFQIALNYGGRAEI
                     VDTVNRIMAVLREREMSDAQIDEQFFSDHLYTANIDDPDLLIRTSGELRVSNFLLWQI
                     AYAEIHVTKVLWPDFRRRDLFEAIIDFQSRDRRYGAVDESDAQMFIEADGKQS"
     gene            15723..>16042
                     /locus_tag="VNN25_01925"
     CDS             15723..>16042
                     /locus_tag="VNN25_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90309.1"
                     /translation="MKRELTALIAAPLAIWIVGWANPYVFDAAIALIAALALYEFLVL
                     GERKGYRLPVALCLVIMMFIVAAFILESVSVEMGVFVALLLIPASYVVSRVDLEKALP
                     SSAIA"
BASE COUNT         3102 a         4967 c         4867 g         3106 t
ORIGIN      
        1 gtctcttgtc tcttgtctct tgtctcttgt ctcttgtctc ttgtctcttg tctcttgtct
       61 cttgtctctt gtctcttgtc tctgatctct ttatccaaaa cccatccgca tcctgaacgt
      121 agaaccaatc aacatggaac gagtccgcac cgcacaaccg cttcgcctct tcagcgtagg
      181 cgcgattgcg tacaccgcga tcgcaatcct gctggtgcgg tcgcaggtgt acgcgacgca
      241 tcccgacgtt ctggcctggg gtctcacctt cgacctcggc atctccattc cgctgatcta
      301 ttggttcctt ttcgtgcgca ccggcgtgat gcggccgatg acgctgatcc cactgttcgt
      361 gatcgctgtc ggcatcgcat cgcgcatcgt gcctcccggg cagcatcact tcgcccagca
      421 actcggctac gtcgccgcgc cgctcgatct cgtgactctg tggatggtgg cgcgccggct
      481 gatgcgggct cgcagcgcgg cagccgaaga tagcgacgtt cccacgcgga tcgcgcagat
      541 cacgcgccag ttgttcggtg acggcgccgt cgcaggcttc gttgcgctcg aaatcagctc
      601 gatctactac ggcctcttct gctggagaaa gcgtgccgcg cccgggttca gcatctacaa
      661 acgcagcggc tgggacacgg tgctcatctg catcttggtg ctgatcacag ccgagtcgat
      721 cggcgttcat ctgctgctcc aggcgtggtc tgtccgcacc gcctgggtcg taacccttct
      781 cgatctctac ggcgcgatct ggctcctcgg cgactatcac gccatgcggc tacgtccgat
      841 cacgatcgat gaacagtcgc tgcacgtgcg cttcggattc cgatggagca tctccattcc
      901 gctaaacacc atcgcgtcga tcgagcccat tcgctccgaa tcggattgga aacgcaaagg
      961 cgtgctgaag gtcgcgctgc tggacgagcc tcgcctgctc atccgcctcg atgcccccgc
     1021 gatcgccacc ggcctcgccg gcatccgaaa aacgatcgac gcagtggccg ttctgcccga
     1081 cgacctcgaa gggttcgagg aagcgctgcg gattttgatc cgcagatgac gcagattaga
     1141 cgcagataaa ggccgttact ttctcatctg cgtctcatct gcgtcatctg cggatcagcc
     1201 gtcacgcccg catcgtatcg ggccgccagg tcttgatctg cttcttgatg tcttcgcggc
     1261 ctttcaactc gccgttgacc gcggagcgca cgagagcggc caactcgctg tcgtcgccgc
     1321 agaaacgaag accgatcaac tgactgctgg aaagctcgcc aatgcggccg cgcagatctt
     1381 ccggaagaca gcgttgcagg cgcagcgtct cttcgagccg gatcaagcga tcctgaacgg
     1441 tgagcaccat ctgccgcgtc gtaagcgcga gcaaaaccag cgcaagcgcg aacgcgatct
     1501 cccaccacac cagccgcgcg ccgcgatgca tgatcgcgta gacgattcgg atgatcagat
     1561 tgatgatcag cagcggaagg gcgacgaagt gaaacaacgg taagaaccgg cgatgattgg
     1621 aaaaggactg cgttgcgtcg gccatgagcg tctcctcgcc gctcaggatc gcatggccgt
     1681 caatgtccgg aggcccgctt ccgcggcgca actggaggaa cgacgactcg gatctcgttc
     1741 gatagcccgc cgcatacata ggaggatccc accgcgaaat cggtggacac cgcgtacgcg
     1801 cgcacttcgt atcgataggc gacgccgggc ttcaccgtcg tgtcggtgag cgttcgcaac
     1861 ccactcgggt tacgagcgat caagctgaat gagccctgat ccgtcgcgcg aaggatttcc
     1921 cacgagccgt tgatcgattc cggcgactgc cactgcaaca ccgccttccc tgccgacgcc
     1981 acgacggtaa gcgcgggcgc cgcggcgcgt ccctgcgcac gcgcggagaa gcaggagccc
     2041 actcccgcgg gaacgagctg caggccgagg cggctgtgac cgaagacgag atcgacgggg
     2101 gcggcgccgc aaccatcctg cgaacgcgcc cagagccgga tgtggttcgg cttcgacgat
     2161 ccccaatgca cgggatcgac gtagaagaac ttctgtgtgc gtcccggaag caccgtgaac
     2221 tcggagatcg tgaagtcggc tgtcgagcac tgaagcggat tggcgcacag actgttgggt
     2281 gtgtcgatga gcgtgttgca gaacgctgcg ctcgcatctt cgtagatcgc ctccaccgtc
     2341 agcgtcacga tgccgctggt agcgctgcgg ttctcgatct cgacgtagcc atctccgaaa
     2401 atcgcgtagt cgtccgacgc tccagcggca tcgagctgcg aatcgagcag caactggtac
     2461 ttgccgcacg ccggcgtccc ccccaacccg cccgtcccgc ccgcgccagc ggccgagagg
     2521 atcaggcagt tcaacggctg atgcgcgtcg ccgagcgaac cgactgcgtc gatgtcactc
     2581 catggaagga gataactgct gtacgttgcg ggcgcggtag ctgcttcgac ctgcgtcatc
     2641 gagatcgtgg cggcggagtc tccgcgcgct tcgaggtgaa cggggtggga gccgggatcg
     2701 agaatatcgg caaagtgatc ccacaacgcg aggccgccac cttccggcat cacgaccgtg
     2761 aagggctcca tcggaaagcc gtcgacgacg taacgaaagt cgatggtgct ctttgcatct
     2821 gccgccacat aactctgcag atacaacctc gcctcctcgg ctggcgcaac cgcgaacttc
     2881 gtacccacca tcgtccaatc cgtaccgatc gtgaccgcgc gatcggagat gagcgacgcg
     2941 ccgggaaaat gctgcgacgg aaatccctgc gcggtcacga acatcaggcg gaacttgatg
     3001 gtcccgcttt ccgcaagaac gcgcgcgtcg atggccacac ggtgcgcacc ggcggcgacg
     3061 ttcgggagaa cgatgttgat gtcgtcgcca tgagggaacc cagcaagtcc gacgcggaag
     3121 tgaaacaccg cgccgaccgg aacatcgtcg agcagccatc ggtattcgac gtcgacgccg
     3181 ggagtggtct gattgttgag ctgcaggaat gcgcggacca ccactgttgc gtcctgcgac
     3241 gccgtcacgt tcacgcgatt cacctgcgag cagctctcga agatgcgctt ccaaccggcg
     3301 ccggccacga cgtcctggtc gagcagttgc cgttgcgtgc ggatcacttc gaagtacgga
     3361 tacggccctt cctgctcgta ctgatcgggg cagaagttgc agcaaccgac cgtcgcttgt
     3421 gcggggagcg gaagaagcag ggcggcagtc agcgcgagaa gaagagcatt gcggatcatc
     3481 aaaagacgta cttagctgtg gcgtgaacct gcaatagctt tgccgtgagc gacgcgccgg
     3541 gctgcacgcg gaagtgctga ttgacgcgca gcttcacttc gcccctcggc gagttgtcga
     3601 tgagcgactg cggcaattcg acgatcgaga tcgtcagcgg gacctcgccc ggatgctcat
     3661 ccacgatctc gcggatcttg cgcaccgtct cctccgtcat gcgcggatag gcgacctcga
     3721 gcgcgatctc tttcaccttc ttgtcgcgaa tcccgtcgag cggaacgatc tcgagcgcgt
     3781 tgagctcggg cgtgatcggc gcttcgtgaa aggtggcggc gtgggaggcg aaattcgttt
     3841 cgtcgggggt ggggagtggg gtgtcggggg tgggagataa atcgtcatcg tcgggagtcg
     3901 tgaatcgcgt gtcgctgatc gaagacgagt cgtccagcgc ctcgtcatcg agaattggag
     3961 actctgctgc cacacccaac tccccactcc ccaccccccc gaacaaaccc agattgttcg
     4021 cgcggtcccc gtacttctcc gcctcgatct ctttcggatc gcggtcgtcc tcgacatcgt
     4081 cttcgctcac gtcgtagccg gagacgtgcg cgtactcgtc gtcggcgcgc tgtgcgtgct
     4141 gctccgcgga ctgcagcgcg gcgctgcggc cggcctgcat gccgcccgtg tccttgaccg
     4201 cggcggtgag caggacggcg atcccattgt cgagccactt cgcgtatttc gagtagagat
     4261 cggagaagac caccacctcg accgagcctc gctgatcgtc gaggatgaac ttcgccatca
     4321 gcttcccttc attcggtccc ttcttgatct tcgacttctt gatctgcgag acgatgccac
     4381 cgatgttcac gatctcgtca atatgttgat gaagtgtgtc tgtggtggcg ttggcgaata
     4441 tgctcagctc gtccgcgaat ttgttgagcg gatgtccggt gatgtagaag ccgagggttt
     4501 ccttttcgaa gcgcagcttc tcgtcgtcgg accattcggc gagtttgtgc aacgcggccg
     4561 cttgcgccga tggcaaaggt ggcggcgccg gcgcagccat catgccgccg aagagcgatg
     4621 actggccgcg ctccttctct tcccgggcgc gctgcgcgga gtcggccgtg ttctcgagcg
     4681 tctcgaacag gaccttgcgc ggcgtgccga gaaaatcgaa cgagcccgac ttgatcaacg
     4741 cttcgatcac cttcttattg caggcgcgaa ggtcgacagc ctcgcagaag tcaaggagcg
     4801 acgcgaacct ccccttccgg tgccgcgctt cgaggatcga ttcgatcgcg ctgcctccca
     4861 cccctttcac ggcgccgagt ccgaagcgga tgttcgggcc gacgacggtg aaggcgtaat
     4921 tcgattcgtt gatgtcgggc ggcagaatgc ggatgcccat ctgcgacgct tcgttgatga
     4981 acttcacgac gaagtcggtg cggtccattt ccgacgtcat cagcgctgcc atgaagtgcc
     5041 gcgggaaatg aaccttgagc cacgctgtct gccaggccac gagcgcgtag gcgaccgagt
     5101 gcgacttgtt gaagccgtag cgcgcgaagg gctcgatgta gtcgaacaga tcgttcgcct
     5161 tcttcttgtc gatgcctttg gcgactgcgc cggtgacgaa cttatcgcgc tccgccgcca
     5221 tgaccgcggc gtctttcttg cccatcgcct tgcgcagcaa gtcggcttgt ccgagggagt
     5281 agccggccac ctgttgggca atctgcatga cctgctcctg gtagacgatg acgccgtacg
     5341 tctcttccag gattcccttc atcgccggca cgaggtactt cgccttcgac agtccgttct
     5401 tgcggcgcac gaactcgtcg accatgccgg catcgagcgc gccggggcga tagagcgcgt
     5461 tgagcgccgc cagatcttcg aagcgcgtcg gccgcgactt gcgcaggaga tcgaccatgc
     5521 cacccgactc gaactggaac acgcccttcg ttcgcccttc ctggaacagc cggaagacct
     5581 cgggatcgtc gagcgggagc gtctcgatgt cgatcgtcac accctcgact gccttcgccg
     5641 acttgatggc gtcgtcgatc acggtgagtg tgcgcagacc gaggaagtcc atcttgagaa
     5701 gacccatctt ctcgacgatg cgcatgtcgt actgcgtcac gatttcatcg cggttcgtgc
     5761 ggtagagcgg cacatagttc gtgaccggct ccggcgtgat gacgacgccc gcggcatgca
     5821 tcccggcgtg acgcgaaaga ccttccagcc gcgagccgat ctcgatgatc cgctcgacct
     5881 cgtgatcgtt cttcatcgcc tcgatgagcg ccggcgagtc cttcgccgcg gtcggcagcg
     5941 ttacgtccgg ccccgtgggg atggtcttgg ccactttgtc gacgaggccg tacggcagtc
     6001 cgaggacgcg gccgacgtcg cgcacgacgg acttggccgc catcgttccg aacgtgatga
     6061 tctgcgcgac cttgtccgcg ccgtacttgc ggcgcacgta ctcgattacc tcgccgcgcc
     6121 ggttcatgca gaagtcgatg tcgatgtcgg gcatcgaaag gcgctcggga ttgaggaagc
     6181 gctcgaagag gaggtcgtag tcaagcggat cgacgtcggt gatgcccatc gaataggcga
     6241 cgagcgatcc ggcaccggaa ccacggcccg gtccgaccgg aatgccgttg tccttcgcgt
     6301 acttgatgaa gtcccacacg acgagaaagt agcccgggaa tcccatcgta cggatgatgt
     6361 cgatctcgct ctcgaggcgg tcgtggtacg cctgcacctc gtgttttttc ttcttcgcgg
     6421 cgaagagcgg cgccgccgcc ttcaaccgcc gctccagccc cgcgcgcgct acctcggcga
     6481 agtaacccgc cagatccttc ccctccggca ccgggaaatt cggcaggtga tagcccttga
     6541 cgtcgagcgt gacattgatg cgctcggcga tcttcacggt gttgtcgagc gcttcgccgt
     6601 agccgctgaa gatacggcgc atctcgtcgg gactcttgac gtagaagtcg tcactgtaga
     6661 acttcatccg cttctcgtcg ccgagcgtct tgcccgtgcc gatgcagagg agaacttcgt
     6721 gcgcgaaggc atcgtccttc gagaggtagt gcgagtcgtt ggtggcgacg agacgcagcc
     6781 cggtttcctc acccagcctg gccatcatcg gcacgatctt ctgctggtcg gggatgccgt
     6841 ggtcctgaat ctcgaggaag aagttgtcgg cgccgaggat gtctttgtat tggagcgcgg
     6901 cctctttggc cttggtgagg ttgccgcccg caaggctctg cgagacctcc cccttcaggc
     6961 acgacgagag aacgatcagc ccttcgcgat gctcgcgcag caactccttg tcgatcctcg
     7021 gtttgtaata gaacccctcg gtgtatcccg ccgagacgag cttgacgagg ttcttgtaac
     7081 cctcgttgtt cgcggcgaga acgatgaggt gattgttcgc gccggcgtcg gcctgaccgt
     7141 cttcaacccc tgccttgtcg aagcgtgagc cgtaggcgac atacatctcg cagccgatga
     7201 tcggcttcac cttctccttc ttcatggcgt tgtagaactc gaccgcgccg aacatgttgc
     7261 cgtggtcggt gatggcgcag gccggcatgc ccatcttcgc gacacgcttc gcgagctctt
     7321 cgggccggct ggcaccgtcg aggagggagt actcggtgtg aaggtggagg tgagcgaacg
     7381 acatgagggc gccgcccgga gtttagccgc ccggccgctg caattggcaa cgaggtctgt
     7441 gcactcgcag ctgagggcgt gatttttcta cggctcgtag actcgccgca gctactgcgg
     7501 catggccaga ccccagtagt actggtcgaa gaacggcttc cagtcctgct tcgtcatgaa
     7561 cccgagaagg ccggccacat cggcggtggt tccgaacttt ccctggaagg acttcgtgta
     7621 cgacttcagg aacgtgagga acttctcgtc gccgagctgc ttgtggatgg ccgcgagcag
     7681 gagaggacct ttgtcgtaga gcagttgcgt tctggcagcc ctggccatga agcgatcgcc
     7741 accgatccga ttcgcgtacg ggatcggcgc gatggcggcc gcctgcgtcg ctctggcacg
     7801 ccatgaattg atcatgcggt tgaagaccgc atcgcccttg aacttcttca ggagcaagcc
     7861 ggctgagtat tcggcgaacg tctccgtgag ccattgctct tccggactcg gcatcttcac
     7921 cgcggatccc caatattgat gcgcgatctc atgggcaatg cgctcgttga ttccctgcga
     7981 gaagtactgc tccgcggaca tcgtatagtc ttcgtccgag ggtttgagcg ggttgaaggc
     8041 ctcatttgtg atgaacatcg tggcgtcggg cgcctgccca taaccgtagg tattgacctg
     8101 gatgatgttg aactccttcc acgggaacgg cccgagaaag tattcgtagt attcgatgaa
     8161 gccgaaggtc ataccgatga gggtcttcgc acccttctcg ctggcgattc cgtacgtggc
     8221 gacgcggacg gtcctatcgt cccgcttctc ctcgaagatg tggtacttgc caccttcgac
     8281 aatcggaaac gcgatcggct tctcgacgac gttctcgacg acgttgtaat cgccctcttc
     8341 cttccgagcg atcaaggtac ctggcgcgat cgggatgaac ggctttctga ccttgatcag
     8401 cgagtgaacc gtggagtagt ttccggccca gttgcgcgac ttcggatacc aggcaaagtt
     8461 gacgagttgc caggcgttgt ctccttcctc gcggaacaga aagtcgccgt cgatgacgaa
     8521 cttcagcttg aacggctgtg tcgccgccgc cggcagcacg acgaggagat tgtcgatttc
     8581 gtgcgcgaag gaaacaggct ttccctgctc atcgaccacg gaccggacat tgattcttcg
     8641 agccggtttg ttggcgacga cgattagctc cgtatcgagg ttgaaacgaa atgccctttg
     8701 gcctccgaag cgtggcgcga tcgtttccgt catctccagc ttcgcgttat cgccgtcggt
     8761 ggtaagcgaa tagtcgagcg ccgtgatcgt aaacgccggc cttgcgtacg ctcgcacgtc
     8821 ccgtcccacc ggctgcgacg atagaatcgt tgggaagaga taggcgctga gggtcttgtc
     8881 agaaacatag ctgttccaga tatgcaccga cgtcagcgac tcctcctgca gatcagccga
     8941 gtcgtaccgg taaaccagct tgtccttcgg cgtgacgatc tcgatgtacg ccgccgctcc
     9001 cggcagaccc agtttctgtg cggcgatcaa gtgctctccc ggttcgatct gcgcgaattt
     9061 gaagacagcc tgatgggcct ttaagtcatc cgcgagcgaa gttcccgcag ttgaagtgcc
     9121 gaggtcaggg ctggtggcgc caaccagcat caccatcgcc tctgtcacgt cttcgctgat
     9181 cgtggcgccg gcaagagtgg cgtgcgagtc gctcttcacg ttccgcgtta ccaccggcat
     9241 ctcggtcgtc tcggccacgt attcgaatcg gccggcgccc ttgaagaaga agcccaccgg
     9301 ctcgccggct gcgctgagct ttgcagccga tccgctggcc atcatcagct tcagatggcc
     9361 aatcgtgagc gtgacgttgt tcaccgcgac cggttcactg agcgacggcg tgcggagatc
     9421 gttcaaaaga tcggcgccgc tggcttcaaa cgcagaaaca aggcacagga ccgcgatcgc
     9481 ggccagaaca cgcagcttca tggaggtctc cgaggatggg atgccgagga ttgtagccta
     9541 cgaatcgaat gcgacgccgc cgatgggacg ggtcgtgcaa cttacgcggc gcccgttcgc
     9601 cagcggacta agaatcggga gacggaaggt acgacgggag gtggcgccga aagaaatgga
     9661 cggcgtagtg gtattcatgc tgcttcatga aggcttcgaa caactcgcgc tcccggagaa
     9721 tgagcttgga gtaatgattg aacacggccg cgacatcttc gatggtcacg aggccgttta
     9781 ggacgtactg gtctatccga tcgacgtagt aaaagaacca gtcaaaacaa tcgcggatga
     9841 agatttcttt ttcgttgatc tgctggtgca tcggcagcgc gagcgcggcg cggatgtcat
     9901 cggtcgcgat ctcgatcagg cccttttcac cagcatcgga cacgaggacg atcccgtcat
     9961 gcttgacgga gtaatttcgc gtgcaatccc accagtcaaa catctgaacc gccgccgacg
    10021 aatagaagcc gtgatgaatt tcgcccagga ggtcgcgcgc tacggcagcc tgcttccagc
    10081 gcagctctgt cttccgagac tcggtgctca tctgcagctc gcggatgccc ttgtaggcgc
    10141 ctatcccgcc gacgacgatc gtgacggccc agaccacaat ctggacttga gaaaggtagg
    10201 gtgacgttgt gacggcaaga gacagcattg tggccaccgc ggcatcgccg ctcggggata
    10261 cgcaaagtcg aagaacggaa gacggattgt agcctacgaa ccgaatgcga cgccgccgat
    10321 gggacgggcc gtgcgatcga cgcgcgtgtc gagcagttca cggagattgg tcgtctcgat
    10381 gaacatgtgc cgatggcgga cgcagtgcat ggccaccgcg aaaaagagga tggccacaac
    10441 gccgaagaag acattcgatg ccggcgaaac ggaaggcaac ggcgagagct tcgtcgaaac
    10501 gaaaaactcc tgctcgagga tcaggcagta gagcgggaac ggcaggaaaa cgagcgcgga
    10561 gagaacccgg cttgcctgca cggagaagta cggacgcagt gccaggaagg ccagcgaaac
    10621 gaagcagccg agcatcatca gtgccgcgaa atgaagcggc gccagctccg cgtatccgag
    10681 tccatggcgg acgagcatca ccatgcgcgc ctgcagaagg agcgtccagg caagagcgac
    10741 gaccgacgcc gtcgtcgcca acagcgcgaa aagcgccccg aagacattac gcgccgccag
    10801 aagcgccgcc atccggatga ggattgcggc agtagcgatg ccggcgatca acgcaaccat
    10861 gattgcccac gccgccgctt tcgtccccga cacgaaggtc ccgcgaaccg ccgcgaaaac
    10921 catcggcccg ccttccgcct gcgacatcag ccccgcggcc gcacaaagcc ccagaaagag
    10981 gcaaaaacca gtgccggcgt aaaagaagaa cttaggcatg ctgtaaatag agtagcaccg
    11041 gggggtgggg agtggggtgt ggaacgtggg agatcagcaa acaagagtgt cagcggcaag
    11101 acacagcaac gggcaatgtc atgcgttggc cgcctcggtc gccgcgcagc cggacggaac
    11161 gatcgcgaca atccggggac agagaatggc ttcgacggca accgcgcatt tgattctctc
    11221 ccaccgccca caccccacac cccatgcccc tcctcatcgc ctcagcgcgc tttccagcgc
    11281 gcgccggacg tcatcgccga acgcaacgaa gaggacctga tcgatcaatt cgttgccggc
    11341 aaggacgcgt gatgtctcgc cgactgcgat ctccgccgcc gcatcgatcg gatagccgta
    11401 gacgccgcaa ctgatcgccg ggaacgcgat cgtccgcacg tcgttcgcca gcgccagctc
    11461 gaggcttcgc cggtaacacg aggcaagcaa cgccggctcg tcgtgtgtcc cgccatgcca
    11521 gaccgggcct accgtgtgga tcacgtattt cgccgggagc cggaacccgg gagtaatctt
    11581 cgcatcgccg gttgcgcagc cgttgaggag ccggcaggcc gcgagaagct cgggcccggc
    11641 agcgcgatgg atcgcgccat cgacgccacc gccgccaagt aacgacgtgt tggcggcgtt
    11701 cacgatcgca tcgactttca gcttcgtgat gtcgcccggg acgatctcga atcgcgtcat
    11761 ttgctgcggc ccagagcgcg cacgataccg aactcgctct tcgtcaccgg ctggatcgac
    11821 agacgcgagc ctttctttgt cacgaccatc tcctcgaggc cgggcgcgga tttcaacgtc
    11881 tcgagcgaaa cgatgtgcgg gaacttctcg acgaaccgga tgtcgaccat gtaccagatc
    11941 ggattctcct cggttgcctt cggatcgtag tagtcatgcc ccttctgacg ggcgaacttg
    12001 tcgggatagg cctcgcggct gatctccgcg atgcccgtga cgccggaagg atccgcgttc
    12061 gaggcgtaga aaaggacgcg gtcgcccgtc ttcatctcgt cgcgcatgaa attgcgcgcc
    12121 tgatagttgc ggacgccttc ccaactcgac gtgccgtcgc gggccaggtc atcgatcgaa
    12181 taggcggagg gctcgcactt catcagccag tatttcatcg gatcgattgt aatccgccgc
    12241 tcctccgggg gattaagcct gttcgcgctg aggttccttt gtccgccatg atgcgcctac
    12301 ggattcctcc cgcctctgct tctctcattc tcatcgccat ctttgcgagc cggcttgcgg
    12361 cagcagagcc cccttgcgag ctgaactaca ggactgacgg tacgacctcg gagacctctg
    12421 tacttaccag cgttgcaccg gagacagtcg tcgagcgtct gccctttttg ttggcgaagg
    12481 cgggcgttac gatccattgg tcccaacccc ctaaaggcat catcgaagcg gaaggactgg
    12541 acgtcaaagc cgggaaaagc ggcgacgaca cacgcgtcac gttccgctct catctgcagc
    12601 cgccggcgaa cagcgcaacg ctctgccggt acgcatcgct tgctggaagc ccgcccgaac
    12661 cgaaaaggcc tcccgtcgca caagatcctg ccctcatcga acggatgaag gacgatctat
    12721 tgaagttgca caccatcgtt atgcagggca gccgtctgaa taacgtgcag ttctcatctc
    12781 cgagcaattt tcttcagttt gccgtcaccg acatcaaagc ctctccggtc aagaaggagt
    12841 acaaggtttc gatgctgctg ccgcgcagca tatgcgccat ctcccacgag gacatggacg
    12901 acctgacctt ggaaatgtcg ggcgaaggta ggtctgcgcc tcgcacgaaa cctgtgcgcg
    12961 ttgagatgtc gctggtttac gagggcgaag gggcggcatc gcatctcagc gaggcctcga
    13021 ttctcaagat cgagagcacg aagtaacgcc tccgtgcgtc ttctcctcac gattcaatac
    13081 ctcggcacgc gctacgccgg ctggcagtcg cagacgaatg ccactgcaat tcagaacgtg
    13141 atcgagcaag cgctgtcgac catgttcggt ggttccatcc gcatcgaagg ggccggccgt
    13201 acggactccg gcgtccatgc ccgggcgcaa cgcgcacatt tcgacgcacc cttcgacatc
    13261 gagatgcgcg gtttccggcg cggcctgaat gatctgttgc cccgcgacat ccgggtgatc
    13321 ggcgtcgaga gcgtcgcgga cgacttccac tgccgcttca tcacgaagtc gaagacctac
    13381 gtctaccgga tctggaacga cgaagtcgcg gatgtcttcg ccgcggagac gagcgcgcat
    13441 gtcccccagc cgctcgatgt tgccctgatg cacacaaccg cgcagcagtt gcagggccgc
    13501 cacgacttcg caatcttcac cgtagccggt ccggaagtga gctcgaccga gcggacgatc
    13561 acggcgatcg acgtcgttgg caacggtgca gcgatcgcga tcacggtcag cgccgacggt
    13621 ttccttcgct cgatggtccg ccgcatcgcc ggctcgctga tcgagatcgg ccggggcaag
    13681 ttggagccgg aggcaatctg gacggtggct cgctggacgg ctccggcgaa gggtctgacg
    13741 ctgtgggagg ttcgctacta agcgcgttct acgaggagta gcttcgaggt acccgaggta
    13801 cgatattcgc gttatggatg acgatctcaa acgggtcctc gaagaaatgc gtagcgagaa
    13861 cgccgctgct cacgcagaga cgcggcgatc cgttgaaaaa acagccgatg cgctgcgcca
    13921 ggaaaacgcg gctgctcacg cagagacgct acgacatgta gaaatgacga tcgaagcgct
    13981 gcgtcaggaa aatgcggccg ctcacgcaga aacgcgaagg ttcgttgaaa ctacagccga
    14041 cgttctgcgt caggagaacg gcgctgcgca cgccgagacg ctacggctcg tagagtctag
    14101 gaccgatgcg ttacgtcagg agatacgcga agagagtgcg gcagctcacg cggaaacccg
    14161 tcgatacgtt gagactacaa ccaacgcgat gcgacaggag atccgcgaag agagtgcggc
    14221 agcccacgcc gatactcggc tgctgttcgg agccacggcc gaagacatgc ggcgcctcgt
    14281 aaagaatgtg gccgacggtg tgacgttggc caatgagaag atcgaccgct tcgacgagaa
    14341 gatcgagcgc attgctgccg gactcgaact ccgcattatt cgtctcgaag tggagagctc
    14401 aagacgatga gcgaaatcaa accgataccc tatgcccaga gatccgccat cgaacaggag
    14461 gagtgggatt cgaggtacga ggaggtcatg gcgcgcgtga gggagatcgc caaccccggc
    14521 gggcggaatc ctccgcgttc tcccgaggaa gtgatggaaa gcgtgcgagc catcgccgaa
    14581 ggaggcgggc ctcgtcggtg gaaatcggaa gaagagctct ggcgaatcat tgcccagatc
    14641 gcacgtggcg gcccagccac tcttgagtaa tgcgcggctc ggccgcaccg gggcggcggc
    14701 ccgcacgagc gcaggcgcct gacgcaagcc atgtgaatcg caggagcatt gcaacctgcg
    14761 acgcacgatc aacagaacgc gcgcgcggct ttgcgatacc atccccggct ccagatgatc
    14821 gatctgcgaa aactcgctga accgggctcg atcgaggaaa cactcctcga caaaatcgac
    14881 ttcaagcgcc tgcctcggca catcgccgtg atcatggacg gcaacggccg ttgggcgcgg
    14941 tcccgcaatc ttccacgcgt cgaaggacat cgggccggga tcgccgcggt gcgcgagatc
    15001 gtcgagactg cggcccgcct cgagcttggc gtcatcacgc tctacgcgtt ctccgtggaa
    15061 aactggaagc gcccgcggtt cgaagtggcc accttgatga tgctgctcaa ggagtacctc
    15121 cgcaaagagt tggccacgct gatggacaac aacatccgct tcaccgccat cggacgcgtc
    15181 gagggtctcg atccttcggt gcaacgcgag ctcaaatacg cggcggatca gaccgcgcgc
    15241 aacagcggcc tgctcttcca gatcgctctc aactacggcg ggcgtgccga gatcgtcgat
    15301 acggtcaacc gcatcatggc cgtgctgcgc gagcgcgaga tgagcgatgc ccagatcgac
    15361 gagcaattct tctccgatca tctctacact gccaacatcg atgatcccga cctcctcatc
    15421 cgcacgagcg gcgagttgcg cgtctccaat ttcctgctct ggcagatcgc gtacgccgag
    15481 attcacgtca ccaaagtgtt gtggccggac ttccgccgcc gcgatctctt cgaagccatc
    15541 attgattttc aatcccgcga ccgacgctac ggtgccgtcg atgaatccga cgcccagatg
    15601 ttcatcgagg ccgatggcaa gcagagctga gaagagatca gcgacgagag atcagagatc
    15661 gggtgctcat cctgaccccg gaactcctct gatctcgaat ctctcgtctc tgatctctat
    15721 ctatgaagcg tgagctgaca gcactcatcg ccgctcccct ggccatctgg atcgtcggct
    15781 gggcgaatcc gtacgttttc gatgcggcca tcgcgctcat cgccgcgcta gcgctgtacg
    15841 agtttctcgt gctgggagaa cggaagggct atcgccttcc ggtagcgctc tgcctcgtga
    15901 tcatgatgtt catcgtcgcc gccttcatcc tcgagtcggt ctctgtcgag atgggagtct
    15961 ttgtagcgct actcctgata ccggcgtcct acgtcgtgtc gcgtgtcgat ctggagaagg
    16021 ccctcccctc gagtgcgatc gc
//
LOCUS       DATLVW010000057         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_291677, whole genome shotgun sequence.
ACCESSION   DATLVW010000057 DATLVW010000000
VERSION     DATLVW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291677"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..>2650
                     /locus_tag="VNN25_01930"
     CDS             131..>2650
                     /locus_tag="VNN25_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH90310.1"
                     /translation="MTLTIMSGLIFAGLWSMKHIALDALPDLSDTQVIIYSKWDRSPD
                     IIEDQVTYPIITSLLGAPKVKAIRGFSDFGYSYVYVIFDEGTDLYWARSRVLEYLSKT
                     QSSLPQGVKTELGPDATGVGWVFQYALVDKSGKHSLADLRSLQDWTIRYQLQSVPGVA
                     EVASLGGFVRQYQITVDPNKLAAYRIPLMQVMSAVRASNNEVGARLVEWSGAEYMVRA
                     RGYVKNVADLEKIVVKTDERGTPVMLRDVANIQLGPELRRGVAELDGQGETAAGIVVM
                     RQSEDALAVIARVKERLAEIEKTLPEGVKIVTTYDRSELIERSIATVKHELLIEIIIV
                     SLIIVIFLWHFPSALIPIVTIPAAVILSFIPMKIFGLNANIMSLGGIAIAIGALVDAS
                     VVVVENAHKKLEVWEADGRKGDYKELLIHAVQEVGRPSFFSLLVIALSFTPIFALVAQ
                     EGRLFRPLAFTKNFAMAIAAVLAITLAPAMMMLVIRPNQKPETRNQKRGPSRFGAVTR
                     LASGFWFLVSGLFNAVLVGKIHPEEKHPISKILFKLYTRPAKLVLRHPRTVIGIAVMI
                     VIFTIPVYMKLGHEFMPPLNEGSLLYMPTTLPGISVAQAEGLLQRQDQLLKEFPEVER
                     VFGKAGRAETSTDPAPFSMMETIVVLKPQTAWRTKARWYSNWPDFTKPMFRPIWPDTI
                     SWDELVNEMNARLTLPGQTNAWSMPIKNRIDMLTTGIRTPVGVKIFGSDLAEIEKIGQ
                     HLEGILQKIPGTRSVFGERVAGGYFVDFDLNRGEIARHGLTIGEVQEVIMTAIGGDNV
                     STTVEGRERYPINIRYPRELRDDIDNLSRTLVSTMGGAQIPL"
BASE COUNT          522 a          822 c          787 g          519 t
ORIGIN      
        1 cacgcctgaa ggcggcgctg gcgcagatga acgcgcccgg aggccaccag cacggacagt
       61 agccggcatt gtgcgtggag acaccagcat gatcgaaaag ctcataaaaa tctgcgccac
      121 gaacaggttc atgaccctga ccatcatgtc cgggctcatc ttcgccggcc tctggtcgat
      181 gaaacacatc gcgctcgatg ctctgcccga cctctccgac acgcaggtca tcatctattc
      241 gaagtgggac cgcagccccg acatcatcga agaccaggtc acgtatccga tcatcacctc
      301 gctcctcggc gctccgaagg tcaaggccat ccgcggcttt tccgacttcg gttactcgta
      361 cgtctacgtc atttttgacg agggcacgga cctctactgg gcgcgcagtc gtgtcctcga
      421 atatctttcg aagacgcaat catccctgcc ccagggcgtg aagacggagc tcgggcccga
      481 tgccaccggc gtcggttggg tctttcagta cgcactggtc gataagagcg gcaagcactc
      541 gctcgcggat cttcgttctc tgcaggactg gaccatccgc tatcaacttc agtccgtgcc
      601 aggcgtcgcc gaggtcgcct ccctcggggg attcgtccgg cagtaccaga tcacggtcga
      661 ccccaacaag ctggcggcct accggatccc gctgatgcaa gtgatgagtg ccgtgcgcgc
      721 atcgaacaac gaggtcggcg cgcggctggt cgagtggagc ggagccgagt acatggttcg
      781 ggcccgcggt tatgtgaaga acgtcgccga tctcgagaag atcgtagtca agacggatga
      841 acggggtacg cccgtgatgc tgcgggatgt ggccaacatt cagctcgggc ccgaactgcg
      901 gcggggagtt gccgagctcg atggtcaggg agagacggcc gccggcatcg tcgtcatgcg
      961 ccagagcgag gacgcgctcg ccgtgatcgc ccgcgtcaaa gagcgccttg ccgaaatcga
     1021 gaaaacgctt cccgagggtg tgaagatcgt taccacctac gaccggtccg agctgatcga
     1081 gcgctcgatc gccacggtga aacatgagct gctgatcgag atcatcatcg tctcgctcat
     1141 catcgtcatt tttctctggc actttcccag cgctctgatc ccgatcgtga ccatcccggc
     1201 cgcggtcatc ctctccttca taccgatgaa gatcttcggg ctgaatgcca acatcatgtc
     1261 gctcggcggg atcgccattg ccatcggtgc tctcgtcgat gcttcggtcg tcgtcgtcga
     1321 gaacgcgcac aagaaactgg aggtgtggga ggccgatggc cgcaagggcg actacaagga
     1381 gctgttgatt cacgcggttc aggaggtcgg gcgtccgagc ttcttttcgc tgctcgtcat
     1441 cgcgctctcc ttcacgccga tctttgcgct cgtagcacag gagggacgcc tgttccggcc
     1501 gctggcattc accaagaact tcgccatggc catcgccgcg gtcctggcga tcaccctggc
     1561 gcccgcgatg atgatgctcg tcataagacc aaaccagaag ccagaaacca gaaaccagaa
     1621 acgtggaccc tcccgcttcg gggctgtgac tcgtctcgct tctggtttct ggtttctggt
     1681 ttctggtttg ttcaacgcag tgctcgttgg caagatccat cccgaggaga agcaccccat
     1741 ctcgaagatc ctgttcaagc tctacacgcg ccctgcgaaa ctggtcctgc ggcatccgcg
     1801 caccgtgatc ggcatcgctg tgatgatcgt gatcttcacg attccggtct acatgaagct
     1861 cggtcacgag ttcatgccgc cgctgaacga aggctccctc ctctacatgc ccacgacgct
     1921 gccgggcatt tcggtcgcgc aagcggaggg actgctccag aggcaggatc agttgctgaa
     1981 ggagttcccg gaagtcgagc gtgtattcgg taaggccggc cgtgccgaga cctccacgga
     2041 tccggcacct ttctcgatga tggagaccat cgtcgtgctc aagccacaga ccgcgtggcg
     2101 cacgaaggcg aggtggtatt cgaattggcc ggacttcacg aagcctatgt tccggcccat
     2161 ctggccggac acgatttcct gggacgagct cgtgaacgag atgaacgcaa gacttacgct
     2221 gccggggcag accaacgcct ggtcgatgcc gatcaagaac cgcatcgaca tgctcaccac
     2281 tggcattcgc actccggtcg gcgtgaagat cttcggatcg gacctcgctg agatcgagaa
     2341 gatcgggcaa cacctcgagg gcatcctgca gaagattccc ggaacccgca gcgtcttcgg
     2401 tgagcgtgtc gcaggcggct acttcgtcga ttttgacctc aaccgcgggg agatcgcgcg
     2461 gcatggactg acgatcggcg aagtgcagga agtgatcatg accgccatcg gcggcgataa
     2521 cgtctcgacg acggtggaag gacgggagcg ctacccgatc aacattcgct acccgcgcga
     2581 gctgcgcgat gacatcgaca acctctcccg cacgttggtg tcgacgatgg gcggtgccca
     2641 gattccgctc
//
LOCUS       DATLVW010000058         7915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_250096, whole genome shotgun sequence.
ACCESSION   DATLVW010000058 DATLVW010000000
VERSION     DATLVW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250096"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1228
                     /locus_tag="VNN25_01935"
     CDS             140..1228
                     /locus_tag="VNN25_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXH90311.1"
                     /translation="MPTKEQILEALKGVKFPGLSRDIVSFGFVHDIVVEGGNVAFTVR
                     FQTENQNAKEQITRDAEAAVRALPGVANVRMTVDVGSRQAAPMGGAPAEMLPGVKYKI
                     AVASGKGGVGKSTVSTNLALALHALGYSVGMLDADIYGPSQQMMLGIDGRPQIDETDE
                     KIIPMENHGIKTMSLGLITDPDTPVIWRGPMVMKALDQFLTDVKWGELDFLIIDLPPG
                     TGDAQLTLTQKVPLSGAVVVTTPQDVALIDARKGLAMFRKVNVPVLGIIENMSYFICR
                     HCGEREEIFGHGGGRKTAELLGVPFLGEIPIDPKVVVGGDTGAPIVVLDPSSAAAQAF
                     RTLAATVAKQVESGHAGHTHEPSHAHAH"
     gene            complement(1298..1939)
                     /gene="aat"
                     /locus_tag="VNN25_01940"
     CDS             complement(1298..1939)
                     /gene="aat"
                     /locus_tag="VNN25_01940"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXH90312.1"
                     /translation="MSRFPDPRYARGDIVAMGDDLRVETLRDAYRHGIFPWPHEELPL
                     PWFSPRRRTVILFDELHAGRSLRKAQRRSGYRCTIDQAFSDVIAACAEVPRGDEAGTW
                     IGPEIITAYTRLHRAGDAHSVEVWSGEDLVGGLYGVDSGGVFTGESMFHRAPDASKIA
                     LLFLIEHLRERGATWIDCQVMTPHMEALGAREIRRSLFLDMLAKEQASARTLF"
     gene            2142..4736
                     /locus_tag="VNN25_01945"
     CDS             2142..4736
                     /locus_tag="VNN25_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXH90313.1"
                     /translation="MAIGCEALANTPPHAPVLIEPSATIPTDPGDVHMATAPFVDDDP
                     GDTHLCSDWEIRAADLTTVVWSSYCATGVLSVHIHLGDGNFAASGARLLGNTRYIVAV
                     RFRDSSGDPETEWSDWTTQAFMTSAGSSVQPLEIVGILQSPQPRLHDPNGNDIVLSGG
                     ASVSLVSAEGTPLLNFTSAGVSNEIPPSSEDHFVVKAVVSSGALPFSLTESNVDFTDE
                     GGARRTIYLPAVELPASASAGFWISRDGSSFNADADKPPFASLARSADTPWSVLEPGY
                     RIERVVTGLQLPVNIAFVPNPLPDADAPIYYITELYGSIRAVLRDGTLRDYATGLLNF
                     DPTGIFPGSGEHGVVGTAVDPVTHDLFVALVYASAIGDHFCKVIRIYSADGGRTASGS
                     TTILDMVGDPIGASHQISSLTIGPDGKLYVHVGDGFETEVARSVNSFRGKILRINQDG
                     TAPVDNPFYDPSDGIRAADYVLALGFRNPFGGAWRAADASLYEVENGPTTDRLAKVSA
                     GLDYQWDGTDESMLAHAAYTWSPSVAPVNITFVQKETFDGSAFPAEKQDHAFVSESGP
                     TWVAAQTPDGKRIREFAFDGGGNVTSTQPFVEYNGAGYATVAALTAGPDGLYFSDLFP
                     EQGSPIGHGANIYRVSYTGTVAIGAEVTDAAARSVRFSSMVTVPGATNLTWIFGDGSS
                     SKEANPVHVFPSNGPFEVKLSVTGASNQVVDNYTRVRFPNAPGVGLVATYSDGSGMQV
                     TRVDNNIDFDWVDEAPPVPAESFTVVWKGEIVATVSAFYTFDMHTTGTATLNINGKTV
                     IEGDDPGMTNPIFLEAGQHYTFSLETVDTVPTGVTQLLWTAPGMPQAVVPKEAFYTSV
                     SRRRAAGH"
     gene            complement(4821..7106)
                     /gene="clpA"
                     /locus_tag="VNN25_01950"
     CDS             complement(4821..7106)
                     /gene="clpA"
                     /locus_tag="VNN25_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HXH90314.1"
                     /translation="MIARQLQKSFEFALNEAMKRRHEYVTLEHLLYALLHDRDVIDII
                     RACGGDIPLLQKQLDDFMNRTLEQLAEDSEVQPVLTAMLQRVVQYAQLHAQSSGQKEV
                     DTGQMLAAIYQAERSQAVYLLRSQGVNKIDVLQYLSHGIGKDTPAPNPAFVGDEQPAP
                     EQSGDPLKLFAVNLNEKAGKGEIDPLIGRGPELERTVQILGRRRKHNPLFVGEPGVGK
                     TAIAEGLALKITKNEVPAVLKDAVIWSLDMGAVLAGTKYRGEFEQRLKAVVNALLAKP
                     EAILFIDEIHTIVGAGAVSGGTMDASNILKPAIASGKLRCIGSTTYAEYKSSFERDRA
                     LARRFQKIEVGEPTVEETIDILKGLKTYYEKHHNVAFTDDALKLAAELSAKYMHDRHL
                     PDKAIDVLDEAGSRARIREAGAAGAASQPASDAAAPAPATPPTSIGIDDIEYVVARMA
                     KIPPRTVAVSEKERLQSLDADLKRAIYGQDHAVAQVVNAIKISRSGLGQPEKPVGSFL
                     FSGPTGVGKTELARQLATSLGVEFIRFDMSEYMEPHTVSRLIGAPPGYVGFDQGGLLT
                     DAIIRTPYAVLVLDEIEKAHPNLFSILLQVMDHATLTDNNGKKADFRNVVLIMTTNAG
                     AREMSDSSMGFGNTSGEKGKGRGAIERTFTPEFRNRLDAWIAFEALSFETTERVVDKF
                     IEELRVQLRAKNVTLELAEPGRAWLAKNGYDRAFGARPMARLFHSKIKEPLVDAILFG
                     KLQDGGNVVVGASGDTLTLDY"
     gene            complement(7148..7459)
                     /locus_tag="VNN25_01955"
     CDS             complement(7148..7459)
                     /locus_tag="VNN25_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HXH90315.1"
                     /translation="MLPIAELDEAPVAESNEKTEEPPLFKVLLHNDDYTTMEFVVWVL
                     ESVFNMTEEQAIQVMLNVHITGVGVAGIYTFEVAEMKVEKTTALAREQEYPLLATLER
                     A"
     gene            complement(7672..>7915)
                     /locus_tag="VNN25_01960"
     CDS             complement(7672..>7915)
                     /locus_tag="VNN25_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HXH90316.1"
                     /translation="PHYLDPDSLSTHKGETREDRIREFHEENLAPVVGLREGSCLRVE
                     DGVTTLLGEKTARVFRRGREAVEVSPGGIDRYLSES"
BASE COUNT         1459 a         2503 c         2445 g         1508 t
ORIGIN      
        1 gactggatca acgaggtcat gatcacgcgc gggctggcca gtccgcagga catgaacgtc
       61 atctcggtgg tcagctcgta cgaagaggcc ataccgatca tccgtgcctc gtacgaacgc
      121 tacattcagg agaaaacgaa tgccgacgaa ggaacagata ctcgaggcgc ttaagggcgt
      181 caaattcccc ggactctcgc gcgacattgt gtcgttcggg ttcgtccacg acatcgtcgt
      241 cgaagggggc aacgtcgcct tcacggtccg cttccagacc gagaaccaaa acgccaagga
      301 acagatcaca cgcgatgcgg aggcggcggt acgtgcgctg cccggcgtcg cgaacgtgcg
      361 gatgaccgtg gacgtcggct cgcgtcaggc cgcaccgatg ggcggcgcgc cggccgagat
      421 gctgcccggc gtgaagtaca agatcgccgt cgcctcgggg aaagggggcg tcggcaagtc
      481 gacggtctcc acgaacctcg cactggcact gcacgcgctc ggctacagcg tcggcatgct
      541 cgacgccgat atctacggac cttcgcaaca gatgatgctc gggatcgacg gacgtccgca
      601 gatcgacgag accgacgaga agatcatccc gatggagaat cacggcatca agacgatgtc
      661 gctcggcctc atcacggatc ccgacacgcc cgtgatctgg cgcgggccga tggtgatgaa
      721 ggcgctcgac caattcctga ccgatgtgaa atggggcgag ctcgatttcc tgatcatcga
      781 tcttcctccc ggcaccggcg atgcacagtt gacgctgacg cagaaggtgc cgctgtcggg
      841 tgccgtcgtg gtgaccacgc cgcaggatgt ggcgctgatc gacgcgcgca aagggctggc
      901 gatgttccgc aaagtgaacg tccccgtcct cggcatcatc gagaacatga gctacttcat
      961 ctgccgccac tgcggcgagc gggaagagat cttcggccac ggcggcggcc gcaaaaccgc
     1021 cgagctcctc ggcgtcccat ttctcggcga gatccccatc gatccgaaag tcgtcgtcgg
     1081 cggcgacacc ggcgcgccga tcgtggtgct cgatccgtca tcggcagcag cacaagcctt
     1141 ccgcactctt gccgcaaccg tggcgaagca ggtggaatcg ggccacgcgg gccacacgca
     1201 cgagccgtcg cacgctcacg cgcattaaat acttgttcac gcgaagccgc gaagacgcga
     1261 agaaacggtt cttcgcacct tcgcgtcttc gcgtgctcta aaaaagggtt cgcgccgagg
     1321 cctgttcctt cgccagcatg tccaggaaca ggctccgccg gatctcgcgt gctcccaacg
     1381 cctccatgtg cggcgtcatc acctggcaat cgatccacgt cgcgcctcgc tctcggagat
     1441 gttcgatgag gaagagcagc gcgattttcg aggcgtccgg tgcgcgatga aacatcgact
     1501 cgccggtgaa cacgccaccg gaatcaactc cgtacagacc gccgacgaga tcctcgccgc
     1561 tccacacttc gacgctgtga gcgtccccgg cgcgatgaag acgcgtgtag gcagtgatga
     1621 tctccggacc gatccacgtg ccggcctcgt caccccgcgg aacctcggca caggccgcga
     1681 tcacgtcgct gaatgcttgg tcgatcgtgc atcggtatcc gctgcgccgc tgggctttgc
     1741 gcagcgaccg gcctgcgtgc agctcatcga aaagaatcac cgtccgccgg cgcggtgaga
     1801 accacggcag cggcagttcc tcgtgcggcc acggaaagat gccgtgacgg taggcgtcgc
     1861 gcagcgtctc cacgcgcaga tcgtcgccca tggccacgat gtcgccgcgg gcgtagcgag
     1921 gatcgggaaa gcgggacatg gccgctccat tatcggtgga cgttccgggc gggtataccg
     1981 atctgtttcc gaaagcgtac ggattccatt attcgggcac acgattcgct tgctggccag
     2041 tctgattccc tgacgcatcg cggactgatt ctcgcgagca ggagttgacg gtaatgcgga
     2101 cgagctgcag agctcttcgg gcgtgggtgg ttctggtagt gatggcaata ggttgtgagg
     2161 cgctcgcaaa cacaccgcct cacgcaccgg ttctcatcga gccgtcggcg acgattccga
     2221 ccgatcccgg cgacgtacac atggccaccg ctcccttcgt cgacgatgat ccgggggata
     2281 cccatctctg cagtgattgg gagatccgtg ccgcggatct caccacggtc gtctggtcct
     2341 cctactgcgc caccggcgtt ctttccgtgc acatccacct cggcgacggc aacttcgccg
     2401 cgtcgggagc gcggctcctc ggcaacacca ggtacatcgt cgccgtccgt ttccgcgaca
     2461 gcagcggcga tcctgaaacg gagtggagtg actggacgac gcaggcgttc atgacctcgg
     2521 cgggttcgtc cgtgcagccc ctcgagatcg tcggcatact gcaatcgccg caaccgcgtc
     2581 tgcacgaccc gaatggcaat gacatcgttc tctcgggcgg agcttcagtc agcctcgttt
     2641 ccgcggaagg gactccgctc ctgaacttca cgagtgcagg cgtcagcaac gagatcccgc
     2701 cctcctccga agaccatttc gtcgtcaaag ccgtcgtcag cagcggcgct ctcccttttt
     2761 cattgaccga gtcgaacgtc gactttacgg acgagggcgg cgcgcggcga acgatctatc
     2821 ttcccgcggt cgaactgccg gcgtccgcgt cggccggatt ctggatctca cgcgacggaa
     2881 gctccttcaa cgctgacgct gacaagccgc cctttgccag cctcgcgcga agcgccgata
     2941 cgccgtggag cgtgctggag cctggatatc gcatcgagcg agtcgtgacc gggttgcagc
     3001 tgccggtcaa catcgcattc gttcccaacc cacttccgga tgcggatgcg cccatctatt
     3061 acatcaccga gctatacggg agcattcgcg cggtcctgcg cgacggcacc ctgcgcgact
     3121 atgccaccgg cctgctgaac ttcgatccca ccggcatttt cccaggctcc ggcgaacatg
     3181 gagttgtcgg aacggccgtt gatccggtga cgcacgatct cttcgttgcg ctcgtctacg
     3241 catcggcgat cggcgatcac ttctgcaaag tcatccgcat ttacagcgcg gacggcggcc
     3301 gcaccgcttc gggaagcacc acgatcctgg acatggtggg cgatccgatt ggtgcgtcgc
     3361 atcagatctc cagtctcacc atcggtcccg acggaaagct ttacgtgcat gtgggagatg
     3421 gtttcgagac cgaggtggcc cggtcggtca attcgttccg agggaagatt ctgaggatca
     3481 atcaggacgg cactgcgcca gtggacaatc ctttctacga cccgtccgac ggcatccgtg
     3541 ctgccgacta cgtccttgcc ctcggcttcc ggaatccctt cggcggtgcg tggcgtgccg
     3601 cggacgcctc gctttacgaa gtcgagaatg gtcccaccac ggacaggctg gcgaaagtgt
     3661 cggcgggact cgactaccag tgggacggca ccgacgaaag catgctggcc cacgcggcat
     3721 acacctggtc gccctccgtc gcgcccgtga acatcacctt cgttcagaag gagacgttcg
     3781 acggcagcgc gttcccggcc gaaaaacagg accacgcctt cgtaagcgaa tcggggccga
     3841 cgtgggttgc ggcgcagacg ccggacggta aacgtattcg cgagttcgcc ttcgacggcg
     3901 gcggcaacgt cacctcgacc caaccgttcg tcgaatacaa cggcgccggc tacgcgacgg
     3961 tcgcggcgct cactgccggt ccggacgggc tctacttcag cgacctcttt cccgagcagg
     4021 gaagcccgat cggtcacggt gcgaacatct atcgcgtcag ctataccggc accgtcgcga
     4081 ttggagccga agtgacggat gcggcggcgc gcagcgtcag gttctcatcg atggtcaccg
     4141 ttcccggcgc gacgaatctc acctggatct tcggcgacgg atcgagcagc aaggaggcca
     4201 accccgttca cgtgttccca tccaatggtc ccttcgaggt gaagctgtcg gtcaccggcg
     4261 catcgaatca agtagtcgac aactacacgc gggttcgatt cccgaacgcg ccgggcgtgg
     4321 gtctggtggc gacctatagt gatggaagcg gaatgcaggt gacgcgagtc gacaacaaca
     4381 tcgatttcga ctgggtcgac gaagcgccgc cggtccctgc ggagtcgttc acggtggtct
     4441 ggaaagggga gatcgtcgcc accgtttccg cgttctacac cttcgacatg cacacaacgg
     4501 gcactgcgac cctgaacatc aacgggaaga cggtgatcga aggggacgat ccgggaatga
     4561 cgaatccgat cttcctcgag gcaggtcagc actacacatt cagcctcgag accgtcgaca
     4621 ccgtgccgac cggcgtcacg cagctcctct ggaccgcgcc cggcatgccg caagcggtcg
     4681 ttccgaagga agcgttctac acatcagtga gccggagaag ggcggcgggg cattaatctc
     4741 aaaagcaatc aaccacagag acactgagga cacagagatt tctctgtgct ctctgtgtct
     4801 ccgtggtgat tgtttttgga tcaatagtcg agagtcagcg tatcgccgct cgcgccgacg
     4861 accacgttcc cgccatcctg cagcttcccg aagaggatcg cgtcgacgag cggttccttg
     4921 atcttcgagt ggaacagacg cgccatcgga cgtgcgccga acgcgcggtc gtagccgttc
     4981 ttagccagcc acgcccgtcc cggctccgcc agttcgagcg tcacattctt cgcgcgtagt
     5041 tgcacgcgca gctcttcgat gaacttgtcg acgacgcgct cggtggtctc gaaggagagc
     5101 gcttcgaagg cgatccaggc gtcgaggcga ttgcggaact cgggcgtgaa cgttcgctcg
     5161 atcgcgccgc ggcctttgcc tttttcgcca ctcgtgttgc cgaagcccat cgatgaatcg
     5221 ctcatctcgc gcgctcccgc atttgtggtc atgatcaaca cgacgttgcg gaaatcggcc
     5281 ttcttgccgt tgttgtcggt cagcgtggcg tgatccatga cttgcagcag gatggagaac
     5341 agattgggat gcgctttctc gatctcgtcg agcaccagca ccgcataggg cgtgcggatg
     5401 atggcgtcgg tcaaaaggcc gccctgatcg aagccgacgt aacccggcgg cgcgccgatc
     5461 agccgcgaga ccgtatgcgg ctccatgtac tccgacatat cgaagcgaat gaactcgacg
     5521 ccgagcgacg tcgcaagctg ccgcgccagc tccgtcttgc caacgccggt cggcccggag
     5581 aagaggaacg atccaaccgg cttctccggc tgcccaagtc ccgagcgcga gatcttgatc
     5641 gcgttgacga cctgcgcgac ggcgtggtcc tggccataga tggcgcgctt cagatcggcg
     5701 tcgagactct gcagacgctc tttctccgac accgccaccg ttcggggcgg aatcttggcc
     5761 atgcgtgcga cgacgtattc gatgtcgtcg atgccgatgc ttgtcggagg ggtagccggg
     5821 gccggcgctg ccgcgtcgga agccggctgc gatgccgcgc cggctgcgcc tgcttcgcga
     5881 atccttgccc gcgatcccgc ttcgtcgagt acgtcgatcg ctttgtcggg cagatgacgg
     5941 tcgtgcatgt acttcgcgga aagctcggcg gccagcttca gagcgtcgtc ggtgaaggcg
     6001 acgttgtgat gtttctcgta gtacgtcttg aggcccttga ggatgtcgat cgtctcctcg
     6061 accgtcggtt cgccgacttc gatcttctgg aaacggcggg ccagcgcgcg gtcgcgctcg
     6121 aacgatgact tgtactcggc gtaggtcgtc gaaccgatgc agcgcagctt ccctgatgcg
     6181 atggcgggct tgaggatgtt cgaggcgtcc atcgtgcctc cgctcaccgc acccgcgccg
     6241 acgatcgtgt ggatctcgtc gatgaagaga atcgcttccg gcttggccag cagcgcattc
     6301 acgaccgctt tcaagcgctg ctcgaactcg ccgcggtact tcgttccagc cagcacggcg
     6361 cccatgtcga gcgaccagat gacggcatcc ttgagcaccg ccggcacctc attcttcgtg
     6421 atcttgagcg ccagcccctc ggcgatcgca gtcttgccga cgcccggctc gcctacgaag
     6481 aggggattgt gcttgcggcg gcgcccgagg atttgaacgg tgcgctcgag ctcggggccg
     6541 cggccgatga gaggatcgat ctcgcctttc ccggctttct cgttgaggtt gacagcgaag
     6601 agtttgagcg gatcgccgct ctgttccggc gccggctgtt catcgccgac aaaggccgga
     6661 ttcggtgcgg gagtgtcttt gccgatgccg tgcgagaggt actgcaggac gtcgatcttg
     6721 ttgactccct ggcttcggag gagatagacc gcctgggagc gctcagcctg atagatcgcc
     6781 gccagcatct gaccagtgtc gacttccttc tggcccgacg actgcgcatg aagctgggcg
     6841 tactgaacca cgcgctgaag cattgccgtc agcaccggct gaacctcaga atcctcagcg
     6901 agctgctcta gcgtgcggtt catgaaatcg tcgagctgtt tctggagcag agggatgtcg
     6961 ccgccgcagg cgcggatgat gtcgatgaca tcgcggtcgt gcagcagggc gtagaggaga
     7021 tgttcgagcg tgacgtactc gtggcgccgt ttcatcgcct cgttgagggc gaattcgaag
     7081 gacttctgga gttgtcgggc gatcatggat ctgggtacgg aacgaaaaag ggtgtcggag
     7141 gatttcgtca ggcccgctcc agcgtggcca gcagcggata ctcctgctcc cgcgccagcg
     7201 ccgtcgtctt ctcgaccttc atctccgcca cctcgaatgt gtagattccg gccacgccga
     7261 cgccggtgat gtggacgttg agcatgacct ggatggcctg ttcctccgtc atgttgaaga
     7321 ctgattcgag gacccagacc acgaactcca tggtcgtgta gtcatcgtta tggagcaaca
     7381 ccttgaacag gggcggctcc tcggtcttct cattgctctc agcgactggc gcctcatcga
     7441 gctccgcgat cggaagcatg gggtcacttt aacgcggttc gcttttcgtt gcggggagtg
     7501 gggagtgggt gtggggggtg gggagttgag tgtggaagaa cgctaaattg atctatctcg
     7561 cgctgctcct caatcgagcg ccttctcccc atcgcttctc agatggggag aaggtggccg
     7621 aaggccggat gaggggctgc caagctgaaa ccgctgctca acccaacact ctcacgactc
     7681 cgaaagataa cgatcaatgc cacctggcga cacctccacc gcctctcgcc cccgccggaa
     7741 gacccgcgcc gtcttctcgc cgaggagtgt agtcacgcca tcttcgacgc gaaggcacga
     7801 cccctcgcgt aaccccacca ccggcgccag attctcctca tggaactcgc ggattcgatc
     7861 ctcgcgcgtc tcccccttat gcgtcgaaag agaatcggga tcgagatagt gtgga
//
LOCUS       DATLVW010000059         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_2222, whole genome shotgun sequence.
ACCESSION   DATLVW010000059 DATLVW010000000
VERSION     DATLVW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VNN25_01965"
     CDS             <1..300
                     /locus_tag="VNN25_01965"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HXH90317.1"
                     /translation="AIALTKLDVLDEFDEIPVCTGYNVRGENWKTFPAFAVAHNDYKP
                     VYRTFKGWKSPTVGMTSYDELPQAAKDYVRFIEDETECPATIISTGPRREETIVR"
     gene            complement(303..542)
                     /locus_tag="VNN25_01970"
     CDS             complement(303..542)
                     /locus_tag="VNN25_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH90318.1"
                     /translation="MPKFPVDAPKARVLAALAHLGFKVVREREHIALERVNDDKTRTP
                     LTVPNHRTLRGSTLRSICNQAGISRDEFLRAYDQS"
     gene            complement(542..766)
                     /locus_tag="VNN25_01975"
     CDS             complement(542..766)
                     /locus_tag="VNN25_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017652075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH90319.1"
                     /translation="MSSIKIIVEKHEDGYVAYPVGMRGAVVGEGDTYDEALADVRSAI
                     RFHIETFGATAFADDSPVLEAFLAETEVAV"
     gene            821..1792
                     /locus_tag="VNN25_01980"
     CDS             821..1792
                     /locus_tag="VNN25_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXH90320.1"
                     /translation="MPTAAPPVRPVTLDEIYAARERIRGVITRTPLIKLRHDTAAPEI
                     WLKLDNLQPINAFKIRGAANAVAMLTPEERAKGVWTISAGNAGQGVAFAAREYGIPCS
                     VMAIETAPETKIERMRNLGARIVKAPFDACWKAMEERRFEGMEGSFVHPFDDHNFIAG
                     NASLGLEILEDLPTVRTVIAAIGGGGLVSGIGSAIRQLAPTVRILGAEPETAAPGARS
                     FEKASAQQFEEWQPSFVDGAGGKSMFPRMWERMQGIVDGSIVVTLDETKRAMRMVAEK
                     VRVIAEGAGALAVAAALSGKAGEGPIVAVISGGNIDLKKFAELIGAA"
     gene            complement(1789..2235)
                     /locus_tag="VNN25_01985"
     CDS             complement(1789..2235)
                     /locus_tag="VNN25_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HXH90321.1"
                     /translation="MEAFSGTITRWTGSTAAFGCAMAVIVIWVITGPVFAYSNTWQLV
                     INTGTTIVTFLMVFLIQRAQNTDALAVQLKLDEIVAALEGASNRLISVEDLSEEELKI
                     LRIHYQRLAEMAKRDEKITESHSIEEAEARHNFKEDRRKKKLLAKS"
BASE COUNT          443 a          710 c          735 g          418 t
ORIGIN      
        1 gccatcgccc tgaccaagct cgacgtcctc gacgagttcg acgagatccc cgtctgcacc
       61 ggctacaacg tccgcggcga gaactggaaa acgttccccg cgttcgccgt agcccacaac
      121 gactacaagc cggtctaccg cacattcaaa ggctggaagt cgcccaccgt cggcatgacg
      181 tcgtacgacg agctgccgca ggcagcgaag gactacgtcc gcttcatcga ggacgagacg
      241 gagtgccccg ccacgatcat ttcgacgggc ccaaggcgcg aagagacgat tgtgcgataa
      301 ggctacgact ggtcataggc acggaggaac tcatcgcgcg agatgccggc ctgattgcaa
      361 atcgaccgaa gtgttgaccc tctcaacgtg cggtgattcg gaaccgtgag cggtgtgcga
      421 gttttgtcat cattcacacg ctcgagcgcg atgtgctcac gttctcgtac aaccttgaaa
      481 ccgagatgcg cgagtgccgc gagaacgcgt gctttcggcg cgtcgaccgg aaacttaggc
      541 attacaccgc gacttcggtt tcagcgagga acgcttcgag cactggcgag tcatccgcaa
      601 atgccgtggc tccgaacgtc tctatgtgaa atctgattgc cgagcgaacg tcggcgagcg
      661 cctcgtcgta ggtatcgcct tcacccacga ccgctccgcg catgccgacc ggataggcaa
      721 cgtagccatc ttcatgcttc tcgacgatga tcttgatcga gctcatagct gtcagtatcg
      781 catgacatgg cgggtccgac gccgtctaca atgtcgcccc atgccgactg ccgctccgcc
      841 cgtccgccca gtcacgctcg acgagatcta cgctgcccgc gaacggattc gcggcgtcat
      901 cacgcgcacc cccctgatca agttgcggca tgacacggcg gcgccggaga tctggctcaa
      961 gctcgacaac ctgcagccga tcaatgcgtt caagattcgg ggcgctgcga atgcggtggc
     1021 gatgctgaca cccgaggagc gggcgaaagg ggtctggacg atcagcgcgg gaaatgcggg
     1081 ccagggtgtg gccttcgctg cgcgggagta cggcattccc tgctcggtga tggcgatcga
     1141 gacggctccc gagaccaaga tcgagcgaat gcgcaacctc ggcgcgcgga tcgtcaaggc
     1201 gccgttcgac gcctgctgga aggcgatgga agaacggcga ttcgaaggaa tggaaggctc
     1261 attcgtgcat cccttcgacg atcacaactt catcgcaggc aacgcctcgc tggggctgga
     1321 gatcctcgaa gatctgccga ctgtgcggac cgtcatcgcg gcgatcggcg gcggcggact
     1381 cgtctccgga atcggcagcg ccatccgcca actggcgccg acggtgagga tcctcggcgc
     1441 cgagccggag actgcggcgc ccggcgcgcg ttcgttcgag aaggcctcgg cgcagcaatt
     1501 cgaggagtgg cagccgtcgt tcgtcgacgg cgcgggcggt aagagcatgt ttccgcggat
     1561 gtgggagcgg atgcagggca tcgtcgacgg ctcgatcgtg gtcacgctcg acgaaaccaa
     1621 gcgcgccatg cgcatggtgg ccgaaaaggt tcgcgtcatc gccgaagggg ccggcgcgct
     1681 tgccgtggcc gcagcactca gcggcaaagc aggcgaaggg ccgatcgtcg cggtgatctc
     1741 gggcggcaac atcgacttga agaaattcgc ggagttgatc ggcgcggctt agctcttggc
     1801 gaggagcttc ttctttcgtc gatcttcctt gaagttgtga cgcgcctcgg cttcctcgat
     1861 cgagtgcgac tcggtgatct tctcgtcgcg tttcgccatc tcggcgaggc gctgatagtg
     1921 gatgcgaagg atcttcagct cttcctctga cagatcttcg acggagatca gccggttgct
     1981 cgctccctcc agcgcagcca cgatctcgtc gagcttgagt tgtacggcca gcgcgtcggt
     2041 gttctgggca cgctgaatca agaagaccat caggaacgtg acgatcgtcg tgccggtgtt
     2101 gatcacgagc tgccaggtat tcgagtaggc gaacaccggc ccggtgatca cccagatgac
     2161 gatgaccgcc atggcgcagc cgaaggcggc ggtgctgccg gtccatcgcg tgatggtccc
     2221 cgagaacgct tccaggcgat cgacgacacg gcatctccat cggcgatcgc ctggaagcgt
     2281 tctcggggac catcacgcga tggacc
//
LOCUS       DATLVW010000060         3800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_539890, whole genome shotgun sequence.
ACCESSION   DATLVW010000060 DATLVW010000000
VERSION     DATLVW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539890"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VNN25_01990"
     CDS             <1..1585
                     /locus_tag="VNN25_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90322.1"
                     /translation="LTSETPNLALAPDGRTLCFGALQGGERWLFLRRLDSFDVRRVEG
                     SIDATCPFWSSDGEWIGFSAHGKLWKTNVEGRQAPAALTDVWPTGAVASWLGHTILFA
                     DHGGGRREIYRIADQGGTPVKVTEPKKSEWRHTWPHLLADGHHFLYWSAAANSFDHQI
                     ILASFDSPSTSVLLPNVSNVALIRRDQLVYVRDAELLSQRFDVDRGTLIGEPVLIAKD
                     VEYFYPTGRATFDAANGVIVYRTDTRTGRLVAADRKGVTRLIDDHGPFDRLSLSYSND
                     GKRTAVTVLNRATGLGDIWIYDLSRAVRDRLTNDSGLAITPVWAPDGRSIVYSLAAGT
                     YPHLFRRDLTASASEELLPPGTFQFAGSFSSDGNTLFFEREVPQTKTDIYRLDMHTRK
                     AEPVLNSTFYEEDPQVSADGKWLAFWSDSTGSSEVYLQSLSAGEVHRVHISTNGGTSP
                     RWRGDGQELFYLSSKDVVMSVTPKVAGQWDETNLTELFHLPPDTLRFAPSPDGQSFLL
                     VEGAQGAADSLFHVITDGQ"
     gene            1620..2096
                     /locus_tag="VNN25_01995"
     CDS             1620..2096
                     /locus_tag="VNN25_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH90323.1"
                     /translation="MPARKSSSMFALALATISLIAVAAYAGAPLKGVDVKLGKSPGGG
                     ASARTTDAEGKLDLGVLPAGSYYLMISAPKGTDVTRDPEAQIEIKGATGGTIKKRWDY
                     AKKKAFDAAPADSSARVAAPIGEEKIIFTSDGSHPVVITASTNSAILKSKSNISNN"
     gene            2713..>3800
                     /gene="tilS"
                     /locus_tag="VNN25_02000"
     CDS             2713..>3800
                     /gene="tilS"
                     /locus_tag="VNN25_02000"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXH90324.1"
                     /translation="MSSSVIVEAIRRFLAAHELVQRPIVAAVSGGGDSTALLVALVEI
                     GDIKFTAAHVNHHLRGTESDDDEAYVRELCARYDIPLRVADGTLDHEAVRHRGIEAAA
                     REVRHARLGEIAGDALIATAHQKNDQAETVMMRLMTGGGIAALRGIHPVRDDGIIRPL
                     LEVSRKEIERFLADRNITPRFDRSNANPRFLRNRVRAMLRDFDESVIDNLAAIADQAR
                     QQWPVLEQAIDAAEHVIASEDETLFRSMPENAWLRQALLLRHIRRLDPDARDVSAADL
                     ARLAGETKKTSVTKSLELSKEGDQIVLRHRSNNPTTPDFEIEIEAGTEVLIPEIATKV
                     RIQPTTDNRQRTPPGVQLIQLPKGAKPT"
BASE COUNT          776 a         1253 c         1095 g          676 t
ORIGIN      
        1 cctgacgtcg gagacaccaa acctcgcgct cgctccggac ggccgaacgc tctgctttgg
       61 ggcgctccaa ggaggcgaac gctggctctt tctgcggcgc ctcgactcat tcgacgtcag
      121 acgggtggaa ggatcgatcg atgcaacctg ccctttctgg tcctccgacg gagaatggat
      181 cggattcagc gcgcatggca aattgtggaa aacgaacgtc gaagggcgtc aggcacccgc
      241 tgcgctcacc gacgtctggc cgacaggcgc agttgcatcg tggctcgggc acaccattct
      301 ctttgccgac cacggcggag ggcggcgcga gatctatcgc atcgcggacc aaggcggaac
      361 gccggtcaag gtgaccgagc cgaagaagag cgaatggcgc cacacctggc ctcatctcct
      421 tgctgatgga caccattttc tctactggtc ggcggcagcc aactcattcg accaccagat
      481 catcctcgct tcgttcgact cgccctccac gtccgtgctg ctgccaaacg tctccaatgt
      541 ggcgctgatc aggcgcgatc aactcgtcta cgtccgcgat gccgagctcc tgagccagcg
      601 ctttgacgtc gatcgcggga cgctcatcgg cgagccggtg ctgatcgcga aggacgtcga
      661 gtacttctac ccgacagggc gtgctacctt cgatgcagcg aacggcgtca tcgtctatcg
      721 aaccgacacc cgcaccggcc ggctcgttgc cgcggatcgc aagggagtga cgcggctcat
      781 cgatgaccac ggcccattcg atcgactgag cctcagctat tcgaacgatg ggaaacggac
      841 cgcagtaaca gttctcaacc gcgccacagg cctgggcgac atctggatct acgacctttc
      901 gcgcgccgtc cgagaccgcc tcaccaatga ttcgggcctg gcgattactc ccgtctgggc
      961 tcccgacgga cgatccatcg tctattcgct cgccgcggga acgtatcctc acctctttcg
     1021 ccgcgatctg accgcatcgg cttccgaaga gcttcttccc cctggtacgt ttcaatttgc
     1081 gggaagcttt tcttccgacg gaaacacgct cttcttcgaa cgagaggtcc ctcagacaaa
     1141 gaccgacatt taccgcctcg acatgcacac gcgaaaggcg gagccggtgc tcaacagcac
     1201 gttttatgag gaggacccgc aggtttcagc ggacggaaaa tggcttgcgt tctggtccga
     1261 cagcaccgga tcgagcgagg tgtatctgca gagtctgagc gctggcgaag tgcatcgcgt
     1321 ccatatctcg acgaacggag gaaccagccc gcgctggaga ggggatggcc aggaactgtt
     1381 ttacctttcc agcaaggacg tagtcatgag cgtgacgccg aaggtcgcag gccaatggga
     1441 tgagacaaac ctcaccgagc tttttcactt accccccgac acgctccgct tcgccccgag
     1501 ccccgatggt caatcgtttc tgctcgtcga aggtgcgcaa ggcgccgccg actccctctt
     1561 ccatgtgatc accgacgggc agtagtgcca tactggattc cgatccttag gaggattcca
     1621 tgcctgcacg caagagctct tcgatgttcg cactcgccct cgccacgatc tctttgatcg
     1681 ctgttgcagc ctacgccggt gcgcctctca agggtgtcga tgtcaaattg ggaaagagcc
     1741 ccggtggagg agcttccgcg cgcacgaccg acgccgaagg gaagctcgac ctcggcgtac
     1801 taccagccgg ctcgtactac ctgatgatca gcgcgccgaa aggcactgac gtgactcgcg
     1861 atcccgaggc gcagatcgag atcaaaggtg cgacgggcgg cacgatcaag aaacgctggg
     1921 actacgcgaa gaaaaaggcg ttcgatgccg cgcctgccga ttcgtccgcg cgagtcgcgg
     1981 ccccaattgg tgaagaaaag atcatcttca cttccgacgg cagccacccg gtcgtgatca
     2041 ccgccagcac caacagcgcc atcctcaagt ccaagtcaaa catctccaac aactgacgag
     2101 gagggttggc tgcatctcac actcgtcgag agtctccaat cgagcgcctt cgcccccacg
     2161 cttctcagtg ggggagaagg tggcggaagg ccggatgagg gggcgtctga tgtcgcggca
     2221 cggcttccaa aacgccaagc gcactccggg atacttcgcc aatctctcga atgcgatgtc
     2281 gtattttgca gcacccccct catccgcctt cggcaccttc tccccccgaa aagcacgggg
     2341 ggagaaggcg ctcgattgga aggcgctcca aagaagaagg acgaggtgta gcaggaaggc
     2401 gcaagcgggt gagaaatgcg gggttgctgc ctttcacacc ctcgcaggaa atctcaatcg
     2461 agcgccttct cccccacgct tctcagtggg ggagaaggtg gccgaaggcc ggatgagggg
     2521 gcctctgatg tcgcggcacg gcttccaaaa cgccaagcac actccgggat acttcgccaa
     2581 tctctcgaac aacgcgatgt cgtattttgc tgcacccccc tcatccgcct tcggcacctt
     2641 ctccccccga aaagcatggg gggagaaggc gctcgattgg aaggcgtcga ccaaaaggtg
     2701 tgagaagtgc aggtgagctc gagcgtgatc gtcgaggcca tccgccgctt cctcgccgca
     2761 cacgaacttg tgcagcggcc gatcgtcgct gctgtctccg gcggcggcga ctccacggcc
     2821 ttgctcgtcg cgcttgtgga aatcggcgac atcaagttca ccgccgcgca cgtcaaccat
     2881 caccttcgcg gcaccgaatc ggatgacgat gaggcatatg tccgcgagct ttgcgcgcgt
     2941 tacgacatcc ctttgcgcgt cgctgacggc acgctcgatc atgaggctgt ccgtcatcgc
     3001 ggcatcgagg cggccgcgcg cgaagtgcgt catgcgcggc tgggcgagat cgccggcgac
     3061 gcgctcatcg ccaccgcgca tcagaagaat gatcaggccg agaccgtgat gatgcgcctg
     3121 atgaccggcg gcggcatcgc tgcgctacgc ggcatccatc ccgtccgcga tgacggcatc
     3181 atccgtccgc tgctggaggt gtcgcgcaag gagatcgagc gcttcctcgc tgaccgcaac
     3241 atcactcccc gcttcgatcg ctcgaacgca aatccgcgct ttcttcgcaa tcgcgtccga
     3301 gccatgcttc gcgacttcga cgagagcgtc atcgacaacc tcgccgccat cgccgatcag
     3361 gcgcgccagc agtggcccgt tctcgaacag gccatcgatg ccgccgagca tgtgatcgcg
     3421 tcggaggacg aaacgctatt ccgctcgatg cccgagaacg cctggttgcg tcaggcactc
     3481 ctcctccgcc acatccgaag actcgatccc gacgcgcgcg acgtgtccgc cgccgatctg
     3541 gcgcggctgg caggcgagac taagaaaacc tcggtcacga aatcgctcga gctctcgaaa
     3601 gaaggcgatc agatcgtcct ccgccaccgc agcaacaacc cgaccacccc cgacttcgaa
     3661 atcgagatcg aagccggcac cgaggtgctc attccggaga tcgcaacgaa ggtccgcatc
     3721 caaccgacaa ccgacaaccg acaacgcacg ccgccaggcg tgcaactcat ccaactaccg
     3781 aaaggcgcca aaccaacctt
//
LOCUS       DATLVW010000061         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_334867, whole genome shotgun sequence.
ACCESSION   DATLVW010000061 DATLVW010000000
VERSION     DATLVW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334867"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VNN25_02005"
     CDS             complement(<1..232)
                     /locus_tag="VNN25_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90325.1"
                     /translation="MKRLGLPVLALVLLSCLPQSLLAWNGTGHELVAGIAWDNMSLDA
                     RNKAIALLESAPSNACLLDLFPNDSRPLAVRQR"
     gene            466..843
                     /locus_tag="VNN25_02010"
     CDS             466..843
                     /locus_tag="VNN25_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90326.1"
                     /translation="MTGTATHRPVAFTLALGLLGGAALIVTSSAISRGPIVLLPYALL
                     VLATAVYLRIEHVQHFARRFSLALGSFMVATVVLYLFIGLVAAKSLFVISLWGHAWRL
                     GIMLAIGAALSAAVAQLTATQAS"
     gene            complement(864..1373)
                     /locus_tag="VNN25_02015"
     CDS             complement(864..1373)
                     /locus_tag="VNN25_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90327.1"
                     /translation="MRKISLVILFVFVAPRLMALSPSDAEDRDGRAQLAEMSSSLKEI
                     VVLLKEQATMQRTELLMRRVTLASTELGAAQDRLRRLDQESAALKSEQGELEGMLSRI
                     QSETASSEDARASRQTRMSEIKSRLAAIQDRFSAVTRDTISAQNEIETLRREFQDWRN
                     LLDKALVKP"
     gene            1447..1902
                     /locus_tag="VNN25_02020"
     CDS             1447..1902
                     /locus_tag="VNN25_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90328.1"
                     /translation="MTPATYRILDAVADSFNPLLALIALAIPLLQKPRNLRATIAYYI
                     STGAAIVFVYLVRALDDRYLLWTSIGLDYSTHSAFAASVVVSIGSFYRRWLVPLICAA
                     IAYFALELVMRYHGVADIVSASVLAGSAAFGLHLAATRVLRVPASKLAL"
BASE COUNT          368 a          691 c          573 g          451 t
ORIGIN      
        1 cgcgctggcg gactgcgagc ggcctcgaat cattcgggaa gagatcgaga aggcaggcgt
       61 tgctgggagc gctctccaga agcgcgattg ccttgttccg ggcgtccagc gacatgttgt
      121 cccaggcgat cccggcgacg agctcatgac cggtaccgtt ccaggcaagg agcgactggg
      181 gaaggcatga caggagaacg agtgcaagaa ccggaagacc gaggcgtttc atagaacatc
      241 cttctggccg ccggggaagc ccggttcatc aggtggggcc ggcaacctac ttgaggagag
      301 tccggctgga cacagttccc taccccgccg tgattcgagg gaccgatccg cacatgacgc
      361 agatggacac agatgcagac aagtctcatc tgcgtcattt gcggattgct ttcgataccg
      421 gcaggcgtgc ctgaaacgag atagactgac ggcgcggagg caacaatgac gggaactgca
      481 acgcatcgac cggtggcgtt cactctggct cttggactgc tcggcggtgc tgcgctgatc
      541 gtcacgtcgt cggcaatctc acgtggaccg atcgtgctcc ttccttacgc actgctcgtt
      601 ctcgccacag cggtatatct ccgtatcgag cacgttcagc acttcgcacg aaggttcagc
      661 ctcgccctcg gctccttcat ggtggcgacc gtcgttctct atctcttcat aggactcgtt
      721 gctgcaaaga gcctctttgt catctcgctt tggggtcatg cgtggcggct cggaatcatg
      781 ctggctatcg gagccgcact cagcgccgcg gttgcccagc tgacagcgac gcaggcgtcg
      841 tagctgacgc agaccgcagc gaactacggc ttgaccagcg ctttgtccag cagattccgc
      901 caatcctgga actcgcggcg cagcgtctcg atctcgttct gcgcgctgat cgtgtcccgg
      961 gtcacggcac tgaatcgatc ctgaatcgcc gccagcctgc tcttgatctc gctcattctc
     1021 gtctgacgcg aggcacgcgc gtcctccgag cttgcggttt cggactgaat tctgctcaac
     1081 atcccttcga gctcgccctg ctcgctcttg agcgcggcgg actcctggtc gagcctccgg
     1141 agccgatcct gcgcagctcc cagctccgtc gacgcaagag ttacgcgcct catcagaagc
     1201 tcggtcctct gcatcgtcgc ttgctccttc agcagcacga cgatctcctt gagcgagctg
     1261 ctcatctccg ccagttgcgc gcgcccatcg cgatcctcag catcactcgg cgagagtgcc
     1321 atcagccgcg gcgccacgaa gacaaacagg atgacaagcg atatctttct catggtttct
     1381 gctccaatcg gtcaacactc gaacctcacg atattcgcat aatcctcgcg atcgaaggcg
     1441 taatccgtga ctccggccac ctacagaatc ctcgacgcag tcgccgattc cttcaatcct
     1501 ctcctcgcgc tcatcgctct cgcgattccc ctcctgcaaa agccgcgaaa ccttcgcgcg
     1561 acgatcgcct actacatttc caccggcgct gcgattgtct tcgtctacct cgttcgcgca
     1621 ctcgacgacc gctacctgct ctggacctcc atcgggctcg actacagcac gcactccgcc
     1681 ttcgctgcat cggtcgtcgt atcgatcggc tcgttctacc ggcgttggct tgtccctctc
     1741 atttgtgcgg caattgccta cttcgcgttg gagctggtca tgcggtatca cggagtcgcc
     1801 gatatcgtct ccgcatccgt cctggccggc agcgcagctt ttggtcttca cttggccgcg
     1861 acccgcgtat tgcgggtgcc ggcgtctaaa ctggctctct gaatcatgcg ctatatcgcg
     1921 atcgtcgcca tggcagtctt cgcgagcggc cgtcacggtc atctctgtcg tcagtggacc
     1981 tatccaacat ccgtaagccc gccaaatgaa cccttcccat tcggcgcgga aaggagcgct
     2041 cgatgtcgcc gcgaactctg catgcactga ccctggccgc gat
//
LOCUS       DATLVW010000062         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_457421, whole genome shotgun sequence.
ACCESSION   DATLVW010000062 DATLVW010000000
VERSION     DATLVW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457421"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..889
                     /locus_tag="VNN25_02025"
     CDS             131..889
                     /locus_tag="VNN25_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXH90329.1"
                     /translation="MHTIVIALAAFAAGAINSIAGGGTLLSFPALVWIGVNPVMANAT
                     NTFALWPGSFAAMVGFRRELANVRRWLLLLTIPALLGGATGGVLLLHTSMATFTRLVP
                     FLILGATLLLATQELITRRLGVLAHAHENPSAAWITIVAIFQFLVGIYGGYFGAGIGI
                     LILAALGLIGLTDMHEMNGLKNLLAICINGVAAIYFAWSGAVIWKDGFIMAVAAIIGG
                     YLGARVARHLGRTFVRRSVVVIGLAMGIALFFKR"
     gene            1012..3036
                     /locus_tag="VNN25_02030"
     CDS             1012..3036
                     /locus_tag="VNN25_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH90330.1"
                     /translation="MRKVSQCAAIVLSIAAAASAQLKESITVNHVDVPVAVVDRSGNP
                     IRGLQAKDFEILDQGKVKEIRSFDIVDFASPASVKATSPLNPVARRNFLILFDLTFST
                     PQTVVRAQAAAGDFLKKMVGPRDRVGVATLDIEHGFRLLSAFTTDRRLVVAAIKNPRS
                     FRGFDPLQVAGTNPLEDVAGDLPDAPGSDNALGVSSDLKEILRGSARLEDDYGREKID
                     RQVTALTGLATTLRAVSGQKHIVFLSEGFESRLIQGRDARGGQAEKTEENTAVEAGQT
                     WKVSNDDRYGNSNSLSLITRLTEAARRSDVILDAVDIHGLRSDIDARKGNSPKSNEGL
                     HLLAGSTGGTVFQNSNDLSSDFGRVLKSQEFVYVLGFQTTAEQPGKFHALKVRVNVPG
                     ARVTARTGYYEGGGVGNILERELTSAEVIINDIPQDTIHLESLAAPFPTTGASAQVPV
                     IIEVNGQDLAAAVGTTRRGTAEIYVYAFDEDGLVRDSTYQRLALDLAKLGDSLKNGIK
                     YYATLSLPPGRYAIKSLVNLAETAKKGFVRKDIVVPANGDVAISQPLFFEEPGKWLMI
                     RGSSHDKTNAGYPFVANGQAFIPSAGVNVRRGEPRTFAVFVENASPDELSVESSPKST
                     IVSQTGSGLRSLLLLRLDSADASAAALDVVVRKRGSDDLRKSSIPLTIRQ"
     gene            complement(3081..>3476)
                     /locus_tag="VNN25_02035"
     CDS             complement(3081..>3476)
                     /locus_tag="VNN25_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90331.1"
                     /translation="RRRVPLAVPSSDNCYLVCDPGHKNATVLPHTADRAGIRSLKRHL
                     TVAEIEQYVTRSGSVDEIIAIAEHLEACFECRDRAVAIVDPGTAAHELRVAELPRERR
                     PAMTTSVVVMCIAIVVIVIAILVFVLGLK"
BASE COUNT          652 a         1144 c         1022 g          658 t
ORIGIN      
        1 atccaaccat cgaggcggga cgcctcgatg gccgcgggcg gagacgcccg cactcctcac
       61 gcaaggcgcg cgtctcgacc gactggaata tgaaatgctg tcccgcctct gattcctgat
      121 ctctgactcc atgcacacca tcgtcatcgc cctcgctgcc tttgcggccg gcgcaatcaa
      181 ctcgatcgcg ggcgggggaa cgcttctctc ctttcccgcg ctcgtgtgga tcggcgtcaa
      241 cccggtgatg gcgaacgcca ccaacacctt cgcgctctgg ccggggtcgt tcgcggccat
      301 ggtcgggttc cgccgcgaac tggccaacgt ccgccgctgg cttctgctcc tgacgattcc
      361 cgcgctcctc ggcggggcaa caggcggtgt gctgctgttg cacacctcga tggcgacctt
      421 cacgcgcctc gtaccgtttc tcatcctcgg cgcaaccctg ctcctcgcga cacaagagct
      481 gatcacgcgc cgcctcggcg tgctcgcgca cgctcacgag aatccgagcg ccgcctggat
      541 cacgatcgtc gccatcttcc agtttctcgt cggcatctac ggcggctact tcggcgcagg
      601 catcggcatc ctaatcctgg ctgcgctggg actcatcggc ctgacggaca tgcatgagat
      661 gaatgggctc aagaacctgc tggccatctg catcaacggc gtcgccgcga tctacttcgc
      721 ctggtccgga gctgtgatct ggaaggatgg attcatcatg gcagtcgccg cgatcatcgg
      781 cggctacctc ggcgcgcgcg tcgccaggca cctcggccgc accttcgtcc ggcgatcggt
      841 cgtggtgatc ggtctggcca tgggcatcgc cctcttcttc aaacgctgag tgcgcttgcg
      901 ccgcccaccg acgcttcagt ttggcaaaac ttctttgcaa aatgtcatcg ctacccggtg
      961 tcgtggtacg ggaccgagcg tacgatagtg attcaaacga aggggttccc catgcggaag
     1021 gtctcgcagt gcgcagcaat cgtgctttcc atcgcggcgg cggcttcggc gcaactgaag
     1081 gaaagcatca ccgtcaacca tgtcgacgtt ccggtagccg tcgtcgaccg cagcggcaat
     1141 ccgatccgcg gacttcaggc caaggatttc gagattctcg accagggcaa ggtgaaagag
     1201 atccgctcgt tcgacatcgt cgacttcgcc tcgcctgcct cggttaaagc tacctcgccg
     1261 ctcaatccgg tcgcccgccg caatttcctt atcctcttcg acctcacatt ctccacgcca
     1321 cagacggtgg ttcgggctca agccgctgcg ggcgacttcc tcaagaagat ggtcgggccg
     1381 cgcgaccgcg ttggcgtcgc tacgctcgac atcgagcacg gcttccggct tctcagcgcc
     1441 ttcacgaccg atcgccggct cgtggtcgcg gcaatcaaga atccgagatc gtttcgtggc
     1501 ttcgatccgc ttcaggtcgc gggcaccaac ccgctggaag atgtcgccgg tgacttgcct
     1561 gatgcgcccg gatccgacaa tgccctggga gtctcgtccg atctcaagga aatcctccgc
     1621 ggatcggccc gtctcgaaga cgattacggc cgcgaaaaga tcgatcgcca ggtgacggca
     1681 ttgacggggc tggcgaccac gctgcgggcg gtttccgggc agaagcacat cgtcttccta
     1741 tccgagggat tcgaatcgcg gctcattcaa gggcgcgacg cgcgcggtgg acaagccgaa
     1801 aaaactgagg agaacacggc ggttgaagcg ggccagacgt ggaaggtttc taacgacgac
     1861 cgctacggaa actccaacag cttgagcttg atcacgagac tcacggaagc ggcccgccgg
     1921 tccgacgtga ttctcgatgc cgtcgacatt cacggtctgc gttccgacat cgacgcgcga
     1981 aagggaaata gcccgaagtc gaacgaagga cttcacctgc ttgccggttc cacaggcggc
     2041 acagtcttcc agaactcgaa cgatctgtcc tccgatttcg gacgcgttct caaatcgcag
     2101 gagttcgtct acgtcctcgg cttccagacc accgcagagc agcccggaaa gttccatgcg
     2161 ttgaaagtgc gggtgaacgt gccgggggcg cgagttacgg cgcgcaccgg atactacgag
     2221 ggtggcggcg tcggaaacat cctcgagcgc gagctcacga gcgccgaggt gatcatcaac
     2281 gacattccgc aagacacgat ccaccttgaa tcgttggcgg cgccgtttcc cacgacggga
     2341 gcgagtgcgc aggtccccgt gatcatcgag gtcaacggcc aggatctcgc cgccgccgtt
     2401 gggacgacac gtcggggaac cgcggaaatc tacgtttacg ccttcgacga ggacggtttg
     2461 gtgcgcgact cgacctacca gcgactcgct ctcgatctgg ccaagctcgg cgacagcctc
     2521 aagaacggca tcaagtacta cgcgaccctc agcctccctc ccggccgcta cgccatcaag
     2581 agcctcgtca acctcgcgga gacggcaaag aagggattcg ttcgcaagga catcgtcgta
     2641 cctgccaacg gagatgtcgc gatctcccag ccgttgtttt tcgaagagcc gggcaagtgg
     2701 ctcatgatcc gcggcagctc gcatgacaag accaacgccg gctatccctt cgtcgccaac
     2761 ggacaggcgt tcattccctc cgccggagtg aatgtccggc gcggcgagcc gcgcacgttc
     2821 gcggtcttcg tggagaacgc ctcgcccgat gagctctcgg tcgaatcgag cccgaaatcg
     2881 acgatcgtct cgcagacagg gagcggcctg cgatcactgc tccttctccg tctcgattcc
     2941 gccgacgctt cggcagcggc actcgacgtg gtcgtgcgca agcgcggcag cgacgatctg
     3001 cgcaagtcaa gcattccgct gacgattcga caatagcttt caccacaaga ggacggagtt
     3061 gagctcagtg tctctgtggt tcatttcagt ccgagcacga atacgaggat cgcaataacg
     3121 atcacgacga ttgcgatgca catcacaacg acactggttg tcatcgccgg tcgcctctca
     3181 cgcggcaatt ccgctacgcg taattcgtga gcggccgtcc cgggatcgac gatcgcaacc
     3241 gcgcggtccc ggcactcgaa gcaggcctcc agatgctcgg cgatggcgat gatctcgtcc
     3301 acgctacccg agcgggtcac gtactgctcg atctcagcca ccgtcagatg ccgcttcaat
     3361 gacctgattc ctgcgcggtc ggcagtgtgc ggcagaaccg tcgcattctt gtgaccgggg
     3421 tcacaaacga ggtagcagtt atcgctgctg gggaccgcca gcggaaccct tcgccg
//
LOCUS       DATLVW010000063        17324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_415255, whole genome shotgun sequence.
ACCESSION   DATLVW010000063 DATLVW010000000
VERSION     DATLVW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17324
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415255"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VNN25_02040"
     CDS             complement(<1..370)
                     /locus_tag="VNN25_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HXH90332.1"
                     /translation="MLKDFDAALTDRVADAYEAYYDLLRFIARRRFRIPAPDIQPLVH
                     DVFVSFMRHEKVIGDDRRWLIAAISNACRNYWRNLKPGQQLDNEHLDPRSPVDDVLAR
                     HDVSLLFARLPAACRSILRLR"
     gene            812..2365
                     /gene="zwf"
                     /locus_tag="VNN25_02045"
     CDS             812..2365
                     /gene="zwf"
                     /locus_tag="VNN25_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXH90333.1"
                     /translation="MSVVAPAIEANPLREGLEQERVPDASCLVIFGASGDLTQRKLIP
                     ALYSLAHDGLLPPGQTIIGYARPDYTDDAFRMAMRESCDKFARTRPVDEAVWDNFAKG
                     LFYVRGEFSEPEGILRLNARMKECDETRGTCGRRIYYLAVPPQFFPTITELLGREKMV
                     SDPEQGGPFTRAIIEKPFGHDLASAKELNRVAVTTFRERQVFRIDHYLGKETVQNLLV
                     LRFANGIFEPFWNRQYIDHVQVTVAESIGVEKRGPYFETAGISRDIIQNHMLQLVSLI
                     GMEPPVAFEADAVRDEKVKVLRALREFPRGHETNVSVRGQYTDGSVLGEKAIAYRKEV
                     KVDPNSNVETYAALKIFIDNWRWADVPFYLRAGKRLPKRVTDISIHFRPAPYPLFNKL
                     THVTMQPNVLAIRIQPDEGISLKFDSKVPGPTVRTSPVTMEFRYATSFGAEPPEAYER
                     LLLETMLGDSTLFARRDEVETAWAWLDPLLNAWAADPIGPELYSAGTWGPEAADKLIE
                     RDGRKWRRP"
     gene            2502..3575
                     /locus_tag="VNN25_02050"
     CDS             2502..3575
                     /locus_tag="VNN25_02050"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HXH90334.1"
                     /translation="MNDITSADVRVDVNAIEKSLSELWRGQKDGEDAVTRAALWNVVA
                     HTSTSETHALAAETLGRASAIVPQRTIVVRSNPAAEAELTSWISANCHLVGGGKQVCS
                     EEINIVAGGDRIHRVPPVVSALLIPDMPVAFWWIGDLPNEHEEYVELLLEPADRLIVD
                     SVYFDSPADLALVSRIAEQTSTSPADLNWVRLEEWRAATASIFDPPTMRSRLNAIRRV
                     RVIAGSSNHEFFGESVESLLYASWLSAQVGHAVDANGKVEGAAGSIDYVFERRHQSTD
                     VGGIALVEISFEDGACASIARDRDRGVLVANVDGSVTVQSVTRTLEQQMEKLIVRQLK
                     RIDGDRVLRRVLPIAIKLAKRVA"
     gene            3619..4311
                     /gene="pgl"
                     /locus_tag="VNN25_02055"
     CDS             3619..4311
                     /gene="pgl"
                     /locus_tag="VNN25_02055"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXH90335.1"
                     /translation="MNLRIFDTAADALSATARTIMQRVEAGARTIALSGGSTPKPLYA
                     SLGSSPLREELAQFPITWIVVDERYVPIDDPESNAGMMQKTLFANGLSPSHRFLRFRT
                     DLNDPAATARAFEDEWRTLGITTLDLVLLGMGDDGHTASLFPGTPVLEVEDRIAAEVY
                     VPRLDSWRVTLTKPILRAAKLRLVLAPGAKKKPVLEALQSGTDYPVAQATTGVETWWM
                     IDREAAPNSAAP"
     gene            4667..4945
                     /locus_tag="VNN25_02060"
     CDS             4667..4945
                     /locus_tag="VNN25_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90336.1"
                     /translation="MGTKKRGRHKRQGLSRRAKEGGRSFLPGPYLSISVSLVAGQLEQ
                     LDHLAVEIRALSGKWITRSGILTAIIEASLQTAARPDAGGEAEGSGPQ"
     gene            5896..6432
                     /locus_tag="VNN25_02065"
     CDS             5896..6432
                     /locus_tag="VNN25_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90337.1"
                     /translation="MSDTLASGVPAPGDEPVTPTTTHYQEVAKQFLDAFIAAIAQIPM
                     LEEKHASTAQFVQTHSRFSNEFVSTVLTSVVADPQLQNINKFDVVEARDTLQYLDAFR
                     SVIDQVQLFLTNLKFSCATRKARAVFQGLQVYAVAKGLGRDATSASVASLAKIMKRDL
                     NRPGRPLKKKEVPPPTNQ"
     gene            6572..7654
                     /locus_tag="VNN25_02070"
     CDS             6572..7654
                     /locus_tag="VNN25_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HXH90338.1"
                     /translation="MLRHLARHGGYDARVNVSATLRQSVQIVQYRSQAAATVPNGAAI
                     LSSRRRRYVYDANHSHTLPGKLVLDERHTGSTDIEANEAFDGSGITYDFFSRVFRRNS
                     IDGKGMPLISTIHYGEHFENAMWDGKQMIYGDGDGKVFNRFTIAPDVIGHELTHGITQ
                     YTARLVYLNQSGALNEHISDAFGIMIKQWALGQTASQSDWLIGKGLLGPDVHGDAIRS
                     MKAPGTAYDDPALGRDPQPAHMRNYVNSPQDNGGVHINSGIPNNAFYRAAIAIGGDVW
                     LVLGPIWYQVLTVQLFPGAGFQDFANATVKAAGSLFGFGGAVQIAVYDAWSQVGLQAP
                     KALMQKTSKERCRIRFRSRPAPWMQP"
     gene            7731..8126
                     /locus_tag="VNN25_02075"
     CDS             7731..8126
                     /locus_tag="VNN25_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90339.1"
                     /translation="MSTTQRSTSQEHHNEDNPLSFESINAHIKDIYMEALTSPIREAW
                     SPDVEVTPADEAEPVSRTVRFVKSYRAVRPILVALGATPFIPGTWCSVVTTFVIMLDD
                     MSASFKAGKDQLLSDMTPSVAMEPKLPVG"
     gene            complement(8431..9711)
                     /locus_tag="VNN25_02080"
     CDS             complement(8431..9711)
                     /locus_tag="VNN25_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90340.1"
                     /translation="MGTAVAIIMVLKRMAPPDRRLTWQLVIAYALLVTTTRIAYVWYP
                     RYSETASLLYSVVGVVTGVVLFAVSLLVIRTLWPQLLARPRVRLTPLGHDAIETYVKA
                     ILDSNAFITALNESLPRGAADAEYGLDSVPYLLSSIRTRRERLDRSTRGLLRLTVIAA
                     TVTALIISFFAWVLVNDESVGAPRALRSLRENTDSLATSLRYLNSDYRGTTEFARLTH
                     AINAKADTGNLPTKAKLQATIRVGMADEPLADLVAALRPVAGELRYDTPSYGRDVDSA
                     LEALETQNTERRAALERVGQLQPRLSALIDRVSQELGKDQNRVAEVVRRIALGLIVAT
                     FFLAIVRFVANLYRADYNQLLRADFDDILVRSFYVTFKASEGMGNERAAVLSSYIRAL
                     STFEPPKAAAEEKGLSKEDSDLLKEILAAAAKKL"
     gene            complement(10440..10661)
                     /locus_tag="VNN25_02085"
     CDS             complement(10440..10661)
                     /locus_tag="VNN25_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HXH90341.1"
                     /translation="MARRNLTLSEKTPAHPKTGRGYRSSDGHWVPSPQQSPNGPPPGA
                     SAQCNDGTYSFSRHHSGTCSHHGGVRRWL"
     gene            10642..10809
                     /locus_tag="VNN25_02090"
     CDS             10642..10809
                     /locus_tag="VNN25_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90342.1"
                     /translation="MRFRRAIQPFRPCHWALHHVGEQAETSTVSPIARVASVADRALG
                     EECNIAWLFVR"
     gene            complement(11106..11384)
                     /locus_tag="VNN25_02095"
     CDS             complement(11106..11384)
                     /locus_tag="VNN25_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90343.1"
                     /translation="MREPNDWRLINQLTYFTGVELGWRRYRKFREGWDHDHCEFCWAK
                     FAEREDSEVLHEGYATPDEMKWVCGPCFEDFKDLFGWIVVASPDNPVV"
     gene            complement(11832..12374)
                     /locus_tag="VNN25_02100"
     CDS             complement(11832..12374)
                     /locus_tag="VNN25_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90344.1"
                     /translation="MILRALILALAFISVYVPTASAHTLVWDLRAESADLTYTGGFVY
                     DPHTGAITSWTVEVILKTDGTPLVVFTPTNSHVTESPAPGEVGNIKFEFSSDDGGFVF
                     TRTLTLTPAKPLLDAGNVVIPVLGTFQCGGDAVCQTITSGHIDNIADPAAVSALSPLA
                     IVMLGASLVFIVLRASHACG"
     gene            complement(12766..13065)
                     /locus_tag="VNN25_02105"
     CDS             complement(12766..13065)
                     /locus_tag="VNN25_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90345.1"
                     /translation="MAKSSLRTGSISFLAIWAAVWLLFLVMRFSPFDYRVIPGIGMIM
                     LGALGLALLAPIVAIALAGAASVRQPRVPLNWITLGCAIAALFGQGLLFLITKWL"
     gene            complement(13714..14082)
                     /locus_tag="VNN25_02110"
     CDS             complement(13714..14082)
                     /locus_tag="VNN25_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HXH90346.1"
                     /translation="MTLFATAGACLIFIGLVLFLRANAFRARSRMTDGRITRYKEDSD
                     GDVSFSIVTFHDDAGTAHETYGPSGSPLPPIGTVVRVYYDMSDPSRAWTAGSITAWVL
                     PTLFVIAGLATIFAGVVLAR"
     gene            complement(14195..14551)
                     /locus_tag="VNN25_02115"
     CDS             complement(14195..14551)
                     /locus_tag="VNN25_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HXH90347.1"
                     /translation="MKKLAFAIALLVLSSQAAFAQDPLVVNAKTLRLKFENARVRVLE
                     ATLKPGEKEHLHSHPSYLFYVIDGGKVRNHSADGKVSEAEFKAGDVIYRDPLTHWSEN
                     IGTTTIHLILVELKEK"
     gene            complement(14586..15326)
                     /locus_tag="VNN25_02120"
     CDS             complement(14586..15326)
                     /locus_tag="VNN25_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HXH90348.1"
                     /translation="MPIEIGHIEAIFRYPVKSMGGEQLDAANLGWHGLDGDRRLAFRK
                     MNDRSGFPWLTAGKLPDLLLFAPHRRDDAAPEDLPTHVRTPDGEELPLFGEELAAEVG
                     RRHGAPVQMMQMKHGIFDEGSVSVIASDTVQEISRLAGQSVDVRRFRPNIVARLLRSG
                     AFQEDDWVGGVLSFGEGDDAPAVTVTMRDLRCSMLNLDPDSASSAPEMMKAVVRANQN
                     TAGIYGTVTRAGRLAVGQPIFLHAAGSR"
     gene            complement(15413..15793)
                     /locus_tag="VNN25_02125"
     CDS             complement(15413..15793)
                     /locus_tag="VNN25_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90349.1"
                     /translation="MDSPHFQRIPILFDSWYSVLSTALGLFPSSSYLQVTNDVVHVRM
                     GWAFRAAFPKSAVASTEIASRSVVSRGVHGFAGRWLVNGSGQGLVIINLNPVQRCHVL
                     GLRLRLKQLIVSVAEPGALVALLN"
     gene            complement(15820..16425)
                     /locus_tag="VNN25_02130"
     CDS             complement(15820..16425)
                     /locus_tag="VNN25_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90350.1"
                     /translation="MNWPPDSALHADVNPQRGVCLVRYHLAYGAARSGIVGQFTIGRR
                     MVESGVSNGMEDRRRRLFWHGILLFVLGLVTGLIEQKLKNPRMGLAAHLEGLMNGTFL
                     LALGAAWNEVRLSRRMSGLAFGAALYGAYANWASTTFAAVLGTAAMTPLASGTFKGQP
                     WQELLVAFGFVSVALAMLISSGLLLWGFRKCRPAAQDPKSV"
     gene            complement(16443..>17324)
                     /locus_tag="VNN25_02135"
     CDS             complement(16443..>17324)
                     /locus_tag="VNN25_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90351.1"
                     /translation="LTLAAMLFTLGIAQASSPQPRDPQKYTRLLIPFHERVASVAGAW
                     NVNWWIHNDGDTPADAFPLAVGGGLPPPPGPDGPRVFILANPAIGPHGTPHGTNGDAL
                     PAYPASPVVPVVTSGTGAFLYAESARLAQLVIGGSLQFNNGPATALHAIPETSFAIGT
                     RNILPIPLLTGWRYALRIYALPETAGNPRVTVRIYDMQALSLHGEEPLIATIETSLFI
                     PTSRVLPCLEPCDVPGGSFAPASLQLFDLARRDPTAFFSTLRVEIEPQSPDLRWWAVV
                     SATDNVSEAVTLYELER"
BASE COUNT         3586 a         5363 c         5178 g         3197 t
ORIGIN      
        1 agcgcaggcg caggatcgac cggcacgccg ccggcagcct cgcgaagagc agagacacgt
       61 cgtggcgcgc gagcacgtca tctaccggtg agcgcggatc gaggtgctca ttatcgagtt
      121 gctgtcccgg cttcaagttc cgccagtagt tgcggcaagc gttgctgatg gctgcgatga
      181 gccagcgacg atcgtcgccg atcactttct cgtgcctcat gaatgagacg aacacgtcgt
      241 ggaccagagg ctggatgtcg ggcgccggga tccggaaacg ccgccgcgcg atgaaccgca
      301 aaagatcgta gtaggcctcg taagcgtccg cgacccgatc ggtgagtgcg gcatcgaaat
      361 ccttaagcat ggattggcgg atgcgcgaag tatcaccaac cacccctcgg atggcaatcc
      421 ccaattgata acaaagcgag cgctgccggt tctgatccca aagacgagag gcctctccga
      481 acgaacgttc gcccggcttg cctggcgggc ggaagggagg gtctatgctc gattttctcg
      541 tcagtctctg gctggacagc cgcatttcca ttccggacgg ttagtcagtc tttcgctgtg
      601 tcagggatcg ggggcgcgtc tccgatcctt gatttgcgag gaggggtcag agatcagaga
      661 ctagagatca gagactagcg atcagagatc agaccgatca gaccgatccg actgatctct
      721 tgtctctgat tttcctgatc tcttgtctct gatcccccac gcctgcccac ccgctccttc
      781 gcatacaatc gcgccgaacg gaggagcaaa catgtcggtg gtggctcccg cgatcgaagc
      841 aaatcctctt cgggagggtc tggagcagga acgggttccc gatgcgtcct gcctcgtcat
      901 tttcggcgcc tcgggtgatc taacccagcg caagctgatt cccgcgctct actctctggc
      961 gcacgacggc cttcttccgc ccggccagac catcatcgga tacgcccgtc ctgattacac
     1021 cgacgacgcc tttcgcatgg ccatgcgcga gtcgtgcgac aagttcgcgc gtacccgtcc
     1081 ggtcgacgaa gcggtctggg acaacttcgc caaaggcctc ttctacgttc gcggcgaatt
     1141 cagcgagccg gaaggcatcc tccgcctgaa cgccaggatg aaggaatgcg atgaaacgcg
     1201 cggcacctgc ggcagacgga tctactatct cgccgtcccg ccccagttct ttccgaccat
     1261 caccgagctg ctcgggcgcg aaaagatggt ctcggatccc gagcagggcg gcccgttcac
     1321 gcgcgccatc atcgagaagc cgttcggcca cgacctggcg tcggcaaagg agctgaaccg
     1381 cgtcgctgtg acgaccttcc gcgaacgcca ggtcttccgc atcgatcact acctcggcaa
     1441 agagaccgtc cagaaccttc tcgttctccg cttcgccaac ggcatcttcg agcccttctg
     1501 gaaccgccaa tacatcgacc atgtgcaggt caccgtggcg gagtcgatcg gtgtggagaa
     1561 gcgcggtccg tacttcgaga ccgcaggcat ctcccgcgac atcatccaga atcacatgct
     1621 gcagcttgtc tcgctgatcg gcatggagcc gcccgtagcc ttcgaagccg acgccgtgcg
     1681 cgacgagaag gtcaaagtgc tccgcgcgct tcgcgagttc ccgcgcgggc acgaaaccaa
     1741 cgtttcggtg cgcggccagt acaccgacgg ttccgttctc ggcgagaaag cgatcgccta
     1801 ccgcaaggaa gtgaaggtcg atccgaattc gaacgtcgaa acgtacgcgg ccttgaagat
     1861 tttcatcgac aactggcgct gggcggatgt gccgttctac ctccgcgccg gaaaacgcct
     1921 gccaaaacgc gtcaccgaca tctccatcca cttccgtccg gctccctatc cgctcttcaa
     1981 caagctcacg cacgtgacga tgcagccgaa cgtgctggcc attcgcatcc agcccgatga
     2041 aggcatctcc ctgaagttcg attcgaaggt tcccggtcca acggtgcgca cgtctcccgt
     2101 gacgatggag tttcgctacg ccacgtcgtt cggcgccgag cctcccgagg cgtacgagcg
     2161 gctgctgctc gaaacgatgc tcggcgactc cacgcttttc gcgcgccgcg acgaagtgga
     2221 gaccgcgtgg gcgtggctcg atccgctgct caacgcctgg gccgccgatc cgatcggtcc
     2281 cgagctctat tccgcgggta cgtggggccc tgaggccgcc gacaaactca tcgagcgcga
     2341 cggccgcaag tggagaaggc cgtgatgtcg gtttcattcg cgactacaag tttgcgttta
     2401 ttaagtaggc gttttttacg taggtcggac tctctagtcc gacttgctca tgatgtcgga
     2461 ctggagagtc cgacctacgt aaacccccga ctcccgacgc catgaacgac atcacctctg
     2521 ccgacgttcg cgtcgatgtg aatgcgatcg agaagagcct ctccgagctg tggcgcgggc
     2581 agaaggacgg cgaggacgcc gttacacgcg cggcgctctg gaacgtcgtc gcccacacct
     2641 cgaccagcga gacgcatgcc cttgcagccg agaccctcgg gcgtgccagc gccatcgtgc
     2701 cgcagcgcac gatcgtcgtc cgctcgaatc cggctgccga ggcggagctg acgtcgtgga
     2761 tcagcgcgaa ctgccacctc gtcggcggcg gcaaacaggt ctgctccgaa gagatcaaca
     2821 tcgtcgccgg gggagacaga attcatcgcg tgcccccggt cgtcagcgcg ctcctcatcc
     2881 ccgacatgcc ggttgcgttc tggtggatcg gcgatctgcc gaacgagcat gaagagtacg
     2941 tggagttgct cctggagccg gccgatcggc tgatcgtcga ctcggtctat ttcgattcgc
     3001 ccgccgacct ggcgctcgtc tcccggatcg ctgagcaaac ctcgacgtcg cccgcggatc
     3061 tgaactgggt ccgcctcgag gagtggcggg cggccacggc ttcgatcttc gatcctccca
     3121 cgatgcggag ccgcctcaac gccatccgcc gcgtgcgggt catcgcgggc agctcgaatc
     3181 acgagttctt cggcgagtcg gtggagtcgc tgctctacgc ttcgtggctg agcgcgcaag
     3241 tcggacacgc cgtcgatgcg aatggcaaag tcgaaggcgc tgccggctcg atcgattacg
     3301 tcttcgagcg taggcatcag tccacggatg tcggcggcat cgccctggtc gagatctcgt
     3361 tcgaagacgg tgcctgcgcc agcattgcac gcgaccgcga tcggggagtg ctcgtcgcca
     3421 atgtcgacgg cagcgtaacg gtccaatctg tcacccgcac gctcgagcag cagatggaga
     3481 aactgatcgt ccgccaactc aaacgtatcg acggcgaccg cgtgctgcgg cgcgtcctgc
     3541 cgatcgcgat caagctggcg aaacgggtgg cgtgatcacg cgaagtcgcg aaggcataat
     3601 caatccagag aagacctcat gaacctccgc atcttcgaca ccgccgccga cgcactctcc
     3661 gccaccgcgc gcacgatcat gcagcgcgtc gaagcgggcg cacgaaccat cgccctttcc
     3721 ggcgggtcga caccgaagcc gctctacgcg tcgctcggat catcgccatt gcgcgaagag
     3781 ctggcgcaat ttcccatcac ctggatcgtc gtcgatgagc gttacgtccc gatcgatgat
     3841 cccgaatcaa acgctgggat gatgcagaag acgctcttcg caaacggtct ctcaccgagc
     3901 caccgtttcc tccgcttccg cacggatctg aacgatcccg ccgccaccgc ccgcgccttc
     3961 gaagacgaat ggcgcaccct cggcatcacc accctcgacc tcgtcctcct cggcatgggc
     4021 gacgatggcc acaccgcctc cctcttcccc ggcaccccgg tcctcgaagt cgaagaccgc
     4081 atcgccgccg aggtctatgt gccgcgcctc gactcctggc gcgtcacgct gacgaaaccg
     4141 atcctccgag ccgccaagct ccgcctggtc ctcgcccccg gcgccaaaaa gaagccagtg
     4201 ctcgaagccc tccagtctgg gactgactac ccagtcgcgc aagcaacgac cggcgtcgaa
     4261 acgtggtgga tgatcgaccg ggaagcggca ccgaacagcg ccgcaccgta gcgccggcgg
     4321 cccgccggcc gtaccgccgc cggcccggcg gcgaccagac atagcgcagg cgttcggcca
     4381 ccacgcaaag gcaaccacag agacacagag aacacagagg ggatctctgt gacctctgtg
     4441 tctctgtggt taccgggctt tcaacaccac ctcgggtttc cggcaacaga taccgacgga
     4501 aaccatcctc cccgtaactt tctcctggct gagctcgtct cactgatgag ggatgagagt
     4561 cgggcgcggc gatcaatcgg accgcacggg ccggccagtc aggaagtgag cttcgtcatg
     4621 cgcatctcac ggattgaggt ggcgtgtgcc gagggaagga gtgctgatgg gtacgaaaaa
     4681 gcgggggagg cacaaacggc aaggcttgtc gcgccgggcg aaggagggcg ggcgcagctt
     4741 tctgcccgga ccgtacctct cgatcagcgt ctctctcgtg gcgggacaac tcgagcagct
     4801 cgatcacctg gccgtcgaga tacgcgcgct gagcggcaaa tggatcaccc gctcgggaat
     4861 cctcaccgcc atcattgaag cgtcgctgca aaccgcggcg cgcccggatg cggggggaga
     4921 agccgagggg agcggaccgc aatgaggctc aatccgtacg tttgcgtgca ggttgccaca
     4981 ctcatggccc tcacgagtgg agcgccgccg gctcgcgcgg cgggagccgc acgcggttcg
     5041 aatgcgcgtc cgcctcgggt ggagaggccg aaccttcatc cgtccgcaaa aaaggtcaac
     5101 tcctgccgaa aaaggtccgg acgcgatttt aataaggcga aaaccacggc cgaaataggt
     5161 ctcgacctaa tttcacgagt ggaaacccct accgaaattg gtctcgaccc aatttcacga
     5221 gtcaaacccc taccgaaatt ggtctcgacc caagtttcac gagtcaaacc cctaccgaaa
     5281 ttggtccgga ccccacttca cgagtcaaaa cagctactga aattggtccc gacctaattt
     5341 cagtagtcaa aacgaccacc gaaattagtc ccgacccaat ttcagtagtc aaaacgacca
     5401 ccgaaattgg tcccgacctg atttcagtag tcaaaacgac tactgaaatt ggtctcgacc
     5461 tatttcaaga gtgcgaacca ccaccgaaat cgagccggcc actttttttt gaaatacgta
     5521 agaccactcc cgagacacgt gcggaccatc ttccgagagg acaacccggg atccggcagg
     5581 tgcagacctc tccacgagag agcgtcgtcg agattcaacg cccgaacttt ccctcctgac
     5641 aggcctcgcg gtgccttctt acgcacaatt ccctaagtaa tgagattcgt gcctgtagcg
     5701 gtagtttaac aattgtacct atccttttgc caaaccctct tcgtatacag attatcgact
     5761 cgatctttca gggcatgtgc gcaccattgc cccaatgcct gcggcgcaac ggccattgct
     5821 ccgcggtgcc actcgccgcc gtaagcgcgc gtcgcgtctt acgtccggtt ttactaagga
     5881 taaggagaac aaatcatgtc tgacaccctt gccagtggtg tacccgcgcc gggcgacgag
     5941 cccgtcacgc cgaccaccac acactatcag gaggtagcga agcagtttct cgatgccttc
     6001 atcgctgcga ttgcccagat ccccatgctc gaggaaaagc atgcatccac ggcgcaattc
     6061 gttcaaaccc attcgcggtt ttcgaacgag ttcgtctcca cggtgctcac ctcggtcgtg
     6121 gcggatccgc aactgcagaa catcaacaag ttcgacgtcg tcgaagcccg cgacacgctg
     6181 cagtacctgg acgctttccg ttcggtcatc gaccaggtgc agctgtttct gacgaatctc
     6241 aagttcagtt gcgccaccag gaaggcgaga gccgtcttcc agggtctgca ggtctacgcc
     6301 gtcgccaaag gtctcggccg cgacgccacc agtgcttccg tcgcgtcgct cgcgaagatc
     6361 atgaagcgcg acctgaatcg tccgggccgg cctctgaaga agaaggaggt gcccccgccc
     6421 acgaaccagt aacacgcgtg cagtagaaac ccgcacggcc gcgttctcgc gcggccgtgc
     6481 gcaacacgcc aaccacctaa accgctaaga aaaggagttt cgtgtcatgc aaaacataca
     6541 tcatcaagct tgtacggcaa ttccgccgca catgttgcgt cacctggccc ggcatggggg
     6601 ctacgacgcg cgggtgaacg tctccgccac actacggcaa tccgttcaga tcgtgcagta
     6661 ccgctcccaa gccgccgcca ccgtgccaaa cggtgcggcc atactcagca gcagacggcg
     6721 ccgctacgtc tacgacgcca atcacagcca cacgctcccg ggcaaattgg tcctggacga
     6781 gcgccacaca ggcagtaccg atatcgaggc caacgaagcg ttcgacggct ccggcatcac
     6841 ctatgacttc ttctcccggg tcttccgtcg caactcgatc gatggcaagg ggatgccgct
     6901 catctcgacg atccactacg gcgagcactt cgagaacgcc atgtgggacg gaaagcagat
     6961 gatctacggc gacggcgacg ggaaggtttt caaccgcttc acgatcgctc ccgatgtgat
     7021 cggccacgag ctcacgcacg gcattacgca gtacacggcc aggctcgtgt atctcaatca
     7081 gtccggcgcc ctcaacgagc acatctcgga tgcgttcggg atcatgatca agcaatgggc
     7141 gcttggccag accgcatcgc agtccgactg gctcatcggc aagggtctcc tcgggcctga
     7201 cgtacacggc gacgccatcc gctccatgaa agcgcctggc accgcctacg acgaccccgc
     7261 cctcgggcgc gacccgcagc ccgcgcacat gcgcaactac gtcaactcac cccaggacaa
     7321 cggaggcgtg cacatcaact cggggatccc gaataacgcc ttctatcgtg ccgcgatcgc
     7381 catcggcggt gacgtctggt tggttctcgg cccaatctgg taccaggttc tgaccgtgca
     7441 gttgttcccg ggcgccgggt tccaggattt cgccaatgcc acagtcaagg ccgccgggag
     7501 cctgttcggt ttcggcggcg cggtgcaaat cgccgtgtac gacgcgtggt cgcaggtcgg
     7561 attgcaagcg ccgaaagcgc tgatgcagaa gacgtcaaaa gagcgatgcc gcatcaggtt
     7621 ccgcagccgt cccgcgccct ggatgcagcc atgacccgtt tccagtccac caaccaccaa
     7681 cccgcgcgcc gtggcgacgt cccgcgcttg accgatacaa aaggaaaatc atgagcacaa
     7741 cacaacgcag cacctcacaa gagcaccaca acgaagacaa ccctctctcc ttcgagagca
     7801 tcaacgccca catcaaggac atctacatgg aggcactcac atcgcccatc cgggaggcgt
     7861 ggagccccga cgtggaggtg acacccgccg acgaagcgga accggtcagc cgtacggtac
     7921 gtttcgtcaa gagctaccgc gcagtccgcc cgattctggt ggccttagga gccactccgt
     7981 tcattccggg cacgtggtgc tcggtggtga cgacgttcgt catcatgctc gacgacatga
     8041 gcgcaagctt caaagcaggg aaggaccaac ttttgagcga catgacccct tccgtggcga
     8101 tggagccgaa gctcccagtc ggctgagtag caacatcatg aatagctccc acccgccggc
     8161 accggccgtt tcgtccccaa acctacgccc gccgttaact cacggcaaac cgtaaatcga
     8221 gacgatccac gagccgcaat gccgccggcg atatccccaa gcagcaagcc ggagcccgcg
     8281 caagcgggct ccggtgtttt tgggggggaa tgctcagtgg agtgggcgag atcgtccgca
     8341 gaaacctgga tctcatgaac gggacgatcg ctctgttcga ccgcaccaac gagtttctca
     8401 agacgctcgt cggagcgtgt gcctcaccac ctatagcttc ttcgcagcgg cggccaatat
     8461 ctccttgaga agatctgaat cctccttcga aagccctttc tcctccgcag ccgccttggg
     8521 aggctcaaat gttgacaagg ccctaatata gctacttagg accgcagcac gctcgttgcc
     8581 catcccttcg gaagctttga atgttacgta gaagcttcgc acgagaatat cgtcaaagtc
     8641 cgcgcgaagg agttggttgt agtccgcacg atagaggttc gcgacaaacc gcacgatggc
     8701 caaaaagaac gttgcaacga tgaggccgag cgcgatacgt ctaacaacct ccgcgactcg
     8761 attctggtct ttgcccagtt cttgactaac acggtcaatc agcgcgctca aacggggttg
     8821 aagctgtcct acgcgctcga gcgcggcgcg cctttcggta ttctgcgtct cgagagcttc
     8881 cagcgcgcta tcaacatcgc gtccgtagct tggggtgtcg tatcttagct cgcccgcaac
     8941 cgggcgtaga gccgccacta aatcagccag cggttcatcc gccataccga cgcgaatagt
     9001 cgcttggagc ttcgccttgg tagggagatt cccggtgtcg gctttggcat tgatggcgtg
     9061 tgtgagtcgc gcgaattccg tcgttccacg atagtcggaa ttgagatagc gcagtgaggt
     9121 tgcgagagag tcggtatttt cgcgcaaaga gcgtagcgcc ctcggtgcgc cgacagactc
     9181 gtcgttgacc agaacccacg cgaagaaaga gattattagc gccgtcaccg tagcggcgat
     9241 gacggtcaat cgcagcaggc ctctcgtgga ccggtccagg cgttcgcggc gtgtacgtat
     9301 cgaactgagg aggtacggca cgctatcgag gccatattcg gcatcggccg cgccgcgtgg
     9361 caggctctcg tttagcgcag ttatgaatgc gtttgaatca agaattgcct tgacataggt
     9421 ctcgatggcg tcgtggccca gcggtgtaag acgtactcgg ggccgggcca agagctgcgg
     9481 ccagagtgta cgaataacca gaagtgaaac ggcgaagaga acgacgcctg tgacgacgcc
     9541 gaccaccgaa tacagtaagc tggcggtctc gctataccgg gggtaccaaa cgtaagcgat
     9601 gcgcgtcgtt gtaacgagga gggcgtacgc tataactaat tgccaggtta agcgccgatc
     9661 gggcggcgcc atgcgcttga gcaccattat gatggcgact gcggtgccca agactggtat
     9721 cccgccaacc gcaacgttca gcacgagacc ccagtccggc gctccaggcg gtagtaagaa
     9781 gaggtagtcg atcacgaagc cctccgaatt gaattcggtg tttgctgagt atacgtcgag
     9841 aaggagagga ggtacccgtt ctgtgaaggt ggggtggttc gccggcgacg cgacgacgag
     9901 ccaaccgaaa agatctttcg cgttgacccg ggctcgagta ctgccactga cggatcctcg
     9961 gggtcgtaag cgaccgggat ttctcttccg tacgggtacc ggcgcagatc cgactcgacg
    10021 aggcagcgcc aggaatgtct ccatactcag ccgaaccgaa tccgtgttcc tcgaaactcc
    10081 tgaccattga cgacgtagcg gaactgaagg tcaggcttgt atgcagcgcc ttcgtcccac
    10141 tcctgaccaa cgcgctgaat ctttccgaga aagcaaccgg ccgaagcgag acagtccttt
    10201 atgcaaccac tgtaaggagc aagcacgcct gggccaacca ccgaggcgtg acgccccagt
    10261 ggccgcgggc gagacgccca cactccttgg gcgaggagtg cggtcgtctc gaccgcgggc
    10321 actcgggcgt cctcgcccga gggcggtcag ggcggaggtc gaagcctcga cgttcttgct
    10381 tgcttgaagc ggcatctatt cgccgcgcaa actcgtggcc tgcgctttaa ccgctttagc
    10441 taaagccacc gccgaacccc tccgtggtgc gagcacgttc cgctgtggtg ccgggagaag
    10501 ctgtacgtgc cgtcgttgca ttgggccgac gcaccgggcg gtggtccgtt cgggctttgc
    10561 tgtggcgacg gtacccagtg accgtccgag ctccggtagc cgcggcctgt cttcgggtgg
    10621 gccggcgttt tctcggaaag agtgagattt cggcgcgcga tccagccgtt tcgtccgtgc
    10681 cactgggcgc tgcaccacgt tggcgagcag gccgagactt cgactgtttc gccgatcgcc
    10741 agggtggcaa gcgtcgctga ccgtgcgctt ggcgaggagt gcaacatcgc ctggcttttc
    10801 gttcggtaag tcgccgcgac cgctggtgcg gcgagaaggg cgatcgtgag agccagggcg
    10861 aatctgtcgc gccgcgaaag ggtcatgggg ttccttgtag gcggccgggc tccgaatgac
    10921 acgactcgtc ttctcgtctt ctttgcttcg ccggctcgaa cgcgccgggc gcacgaattg
    10981 cgattctcgc cgaatacagc gtcgggacaa gccgcgctga catccggaga atcaacgcaa
    11041 cggtaacacc ggcggcgccg cgggatgaga gcaacggtga gtttattttc tagacacgac
    11101 agcgactaca caacagggtt atccggggac gcgacaacga tccacccgaa aagatctttg
    11161 aagtcttcga agcacgggcc gcagacccac ttcatctcgt ccggtgtcgc atagccctca
    11221 tgtagaacct cactgtcttc acgctccgcg aacttcgccc agcagaactc gcagtgatca
    11281 tggtcccaac cttcgcggaa cttccggtag cgacgccaac cgagctcgac acctgtgaag
    11341 taggtgagtt ggttgatcag ccgccagtcg tttggctctc gcattcgtat tttgacggca
    11401 aacgtttaga gcactggcga ggaaagtcct tcgtactgcc gaacagacgc aagctcaccg
    11461 atccgcgcgg aagagtgtag cgcgcctgag acgcagcaag ttgcggctcc tccgttgccg
    11521 ttagagagct ccaacagctc gtacacgtat tttgcctgtt gccgagggcg tcggagaact
    11581 tgttgatgaa gatgcctttc gcacagcccg gtcaggcctg tcccaggcgt ttcggatatc
    11641 gaggcagccg tgttcaaggt gcctgcgctg ccaaccgcgc cgcagttcgc ccgctgggga
    11701 ctcccgccgc cgttcgtgtc catgccgcac cgtcgatcac gctgctgccc tgtccttcag
    11761 cgtgtacgtc tcgcttccgt tgaccttcgg ccggccgacg tgggcggcgg gcgcgtcgat
    11821 tccgagaatt cctacccgca cgcatgcgaa gcgcggagca cgatgaacac gagactcgct
    11881 ccgagcatga cgatcgccag cggcgaaagc gccgagacgg ccgctgggtc cgcgatgtta
    11941 tcgatatgcc cagaggtaat agtttgacag accgcgtcgc cgccgcactg gaaagtacca
    12001 agcaccggaa tcacgacatt tccggcgtcg agcagtggct ttgcaggtgt aagcgtgagg
    12061 gtccgcgtga aaacaaaacc accatcgtcg gacgaaaact cgaacttgat attgccaact
    12121 tcacctggtg ccggtgactc agttacgtga gagttggttg gcgtgaagac gacaagtggc
    12181 gtgccgtcgg tcttcaatat gacctctacg gtccatgatg taatggcccc agtgtgcgga
    12241 tcataaacaa agccgcccgt ataggtgaga tcggcgcttt cagcgcgaag gtcccagacc
    12301 agcgtatgcg cacttgcggt gggtacgtat acggagataa acgcgagcgc cagaatgagt
    12361 gcgcgtagga tcatgaagtg acacctttag ctttactggt ttgtccgcct caggccgcac
    12421 tgcgacgccc gtgagaatca ccagtcgtgc gtgaagactg caggtgccgc agcgagcggt
    12481 gacgcagcgg catgccgctg atgttgtcca taatattgtt tcgagcaggg cggcgtgcaa
    12541 taggcaatta tggaattggt agcgatatgc ggtgcgtgat gacccgagag ccaaatgaag
    12601 cgcgatgttc tcctggcgcg cattccaggc cgaggttggg tcaggtcaaa agtgaaagta
    12661 acgaaactgg aatcgggcag ccaggcagga tcgattcgtc atgactatta ctttcgacct
    12721 gtccccagcg ttgatctcct ggtccgctcc gccgagagaa aggcatcaca gccacttcgt
    12781 gatgaggaac agaagtccct ggccgaagag agcggcgatc gcgcatccga gggtgatcca
    12841 gttcaggggc acccgaggct gtcggactga tgcggcgccg gcaagtgcta tggccacgat
    12901 cggagccagg agggcaagcc cgagtgcgcc gagcatgatc atgccgatac ctggaatgac
    12961 cctgtaatcg aacggcgaaa agcgcatcac gagaaacaaa agccagaccg ccgcccagat
    13021 ggcaaggaag gatattgacc cggttcgcaa cgaggatttg gccatttctt gcgttgatgc
    13081 ctaacggctc tgcggttcat ccgcacgaag cgcggcgcgg tgaagtatag cgcccgcggc
    13141 acagcaacag gcgcttcaag tgtgttgcag cgccgaggtt gcgccacgca tcaaaagtca
    13201 cgggtggacg ggtaccagcg cggagcaatc tgcgccagca ggctcggaac gtgcgctgag
    13261 gatgtctcga gcaacagcat acagggcctc cgagcgtgat attgaagagc ggctcgctgc
    13321 ttccagtgcg agtcaggccg aggaggtgca ggtggcgatc aggtccgccg tgctcgtacg
    13381 cgactagtat ccgagtgcgg gacacaccga cgagaacaaa tcgcctgctg atgcggcctc
    13441 gctcggtagt acagccggag ttgaacggct gtccggcctc ggccagcggg aagggaagca
    13501 ccttgccgat tcggagcgca atacctctcg gcaccgcatc ctgccgcagg aattctttga
    13561 aggagtctct ctgaaacgcc gcgatcgtct gcgagagcgt tgcaatgtga gttggggctc
    13621 tgagtccggc gacgacgata gcaatggcgg cgaatagcga acgttgcatt ccttctatac
    13681 gaccgaggcg acctccaggt tcccctcgtc cgcctagcgg gccagcacca cgccagcaaa
    13741 gattgttgcc aggcctgcga ttacgaagag cgttggtaga acccaggctg tgatggaccc
    13801 tgccgtccag gccctggacg gatccgacat gtcgtaatac acgcgcacga ccgttccgat
    13861 cgggggcagc ggagaccccg agggtccgta tgtttcatga gcggttccgg cgtcatcgtg
    13921 gaatgtcacg atggagaagg acacgtcacc atcggaatcc tctttgtacc gtgtgatcct
    13981 gccatcggtc atccgactgc gggctcggaa ggcattcgct cgtaaaaaca ggaccaggcc
    14041 gatgaagatc aggcaagcgc cggctgtcgc gaacaatgtc attggattct gcggagattc
    14101 tgcacacaag caaagcaggt caccacagag acacagaggt cacagagtac gcactcaacc
    14161 tcggtgtcct tagtgtctct gtggttcatg tttatcattt ctcttttagc tcgaccagga
    14221 tgaggtggat tgtcgtggtt ccgatgttct ccgaccagtg tgtgagtggg tcgcggtaga
    14281 tcacgtcgcc cgctttgaat tcggcttcgc tgacctttcc gtcagcagag tgattgcgga
    14341 ccttgccacc gtcgatgacg tagaagagat agctcggatg cgagtgaagg tgctccttct
    14401 ctcccggttt cagcgtcgcc tccagcacgc ggacgcgcgc gttctcgaat ttgagccgca
    14461 aggtctttgc gttgacgacg agcggatcct gagcgaatgc ggcctgagac gagagcacca
    14521 acaacgcgat ggcgaatgcg agtttcttca tcacaacctc ctgatgccta tacagacctt
    14581 acagattacc tgctcccggc cgcatggagg aatatgggtt gtccgacagc aagacggccg
    14641 gcgcgggtga cggtgccgta gattccggct gtgttctggt tcgcgcgaac gaccgccttc
    14701 atcatttctg gcgccgagct cgcggagtcg ggatcgaggt tcagcatcga gcatcgaaga
    14761 tcgcgcatcg tgacggtgac ggcgggagcg tcgtccccct cgccgaacga gagcacgccg
    14821 ccgacccagt catcctcctg gaaagcaccc gatcgcagta gacgagccac gatgttggga
    14881 cgaaatcgcc gcacgtccac gctttggcct gccagccggc tgatctcctg caccgtatcc
    14941 gaggcgatca cggagacgct cccttcatcg aagattccat gcttcatctg catcatctgt
    15001 acgggagcgc cgtgccggcg tccgacctcc gcggccagct cctctccgaa gagagggagc
    15061 tcttcgccgt ccggcgttcg aacatgggta ggaaggtctt ccggcgcagc gtcgtcacgg
    15121 cggtgcggag cgaagaggag cagatcggga agcttgcctg cggtcagcca cgggaacccg
    15181 ctgcggtcat tcatcttccg gaatgccaga cgccggtcac catcgagacc gtgccagccg
    15241 aggttggcgg cgtcgagctg ttcgccgccc atggatttca cgggatagcg gaagatggcc
    15301 tcgatgtgtc cgatttcgat gggcatgtct gcgcgtgttg tacgttggat cgtggccgag
    15361 cgtcggggtg aggagacggt ttcacgcgga ggcgcgggga acgcggagag aatcaattga
    15421 ggagtgcgac gagagctcct ggctccgcca cgctcacgat gagttgcttc agccggagcc
    15481 gcaacccgag aacgtgacac cgctgcaccg ggttgaggtt gatgatgact agtccctggc
    15541 cggacccatt caccagccac cgccctgcga acccatgcac tcctcggctc accacactcc
    15601 ggctggcaat ctctgtcgat gcgacggcgc ttttcggaaa tgctgcgcgg aaagcccaac
    15661 ccatgcgaac gtggaccacg tcgttcgtca cttgcaagta cgagctcgaa ggaaacagcc
    15721 cgagtgcggt cgacagcacc gaataccagg agtcgaacag aatggggatt cgctgaaagt
    15781 gagggctgtc catggtgaaa atcgcgtgca gagcaagcgt tagacgctct taggatcttg
    15841 cgctgcgggt ctgcatttcc ggaaacccca tagaagcagg ccggaagaga tcagcattgc
    15901 cagagcaaca gatacaaagc cgaaggcgac cagtaactcc tgccatggct gtcccttgaa
    15961 cgtacccgac gcaagcggcg tcattgctgc tgtgccgagg accgcggcga acgttgtcga
    16021 ggcccagttg gcatatgcgc cgtaaagcgc cgcaccgaat gccaatcccg acatgcgacg
    16081 ggaaagacgc acctcattcc acgccgcgcc caaggcgagc aggaaggtgc cgttcatcag
    16141 tccttcgaga tgtgccgcca ggcccatgcg cgggtttttc aacttctgtt cgatcaggcc
    16201 agttacgaga ccaaggacga aaaggagtat cccatgccag aacaagcgac gtctgcgatc
    16261 ttccatcccg ttcgaaactc ctgattcaac catcctgcgg ccgatagtga actgtccaac
    16321 gatcccagag cgagcggcac cgtacgcgag atggtaacgc accaggcaca caccgcgttg
    16381 cgggttcacg tccgcatgca gcgccgagtc aggcggccaa ttcatcgcgg gtccgcttct
    16441 gctcaccgct ccagttcata cagcgtgacc gcctcggata cgttgtcggt ggctgagacg
    16501 acagcccacc aacgcaggtc gggtgattgg ggctcgatct cgacgcgaag agtcgaaaag
    16561 aacgcagtcg gatcgcgccg cgcgaggtcg aagagctgaa gcgaagcggg tgcgaacgat
    16621 ccgccgggga catcgcacgg ctccaagcac ggcagaacac gcgacgtcgg tatgaagaga
    16681 ctcgtctcga tcgtagcgat gagcggttct tcgccgtgga gcgacagcgc ctgcatgtcg
    16741 tagattctga ctgtcacccg cggattgcct gcggtctcag gcaaggcgta gatccggagt
    16801 gcgtatcgcc accccgtgag caggggaatc ggaagaatgt tccgcgtccc gatggcgaat
    16861 gaagtttcgg gaatcgcgtg cagcgccgtc gccggaccat tgttgaactg gagagagcct
    16921 ccgatgacga gctgcgcgag acgagccgat tccgcgtaga ggaaagcgcc ggtgcccgat
    16981 gtgacgacgg gaacaaccgg agaagccgga tacgccggaa gtgcatcgcc attcgttccg
    17041 tgaggtgtgc cgtgtgggcc gatggctgga ttcgcgagga tgaacacccg cgggccatca
    17101 ggcccaggcg gcggcgggag tccacctcca acggcaagcg gaaacgcatc ggccggggtg
    17161 tcgccgtcat tgtggatcca ccaattcaca ttccaggcgc cggccaccga ggctacacgc
    17221 tcatgaaagg gaatcaggag gcgcgtgtac ttctgcggat cacgcggctg cggcgaagac
    17281 gcttgtgcga tgccgagggt gaaaagcatc gcggccaggg tcag
//
LOCUS       DATLVW010000064        13561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_498724, whole genome shotgun sequence.
ACCESSION   DATLVW010000064 DATLVW010000000
VERSION     DATLVW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13561
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498724"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VNN25_02140"
     CDS             <1..2010
                     /locus_tag="VNN25_02140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXH90352.1"
                     /translation="MTPIAVLFLATSLAAQTAPEHGVFVEDIDKNADACTNFYDYANG
                     AWRAANPIPGSMQKWSRRWKAGEQSKEQLRALLDDESKKKDWPKGSIDQQISDFYASC
                     MDQARVDALGAKPIQPLLHEIKAMRTAADLQKTIADLHDLQMFAAFGITSASDNHNPG
                     YVIARVFASGLGLPDRDYYLKPEKRFADARAKYRVHVAKMFELTGYSAAEAKRASEAI
                     FAMETRLAAAQLDNVALRDPAATDHKYQVATLQKFTPHVDWTRYLDRFKVAPADLNVD
                     QPEYMGAVEKELRSTPLATWKAYLAWNVINSAARHLSSPIAAEEFAFYGKYLSGSTEM
                     KPRWKRCVEGDDMFLGEALGQRYVERYFPPEAKARMKELVKNLLLAMGDTIRGLDWMS
                     PATKTRALEKLATFNPKVGYPDKWKDYSSVVISPTSYWENVEAGLKFDNADSLAQAGK
                     PVDRGRWGMTPPTSDAYYNPLLNEIVFPAGILQPPAFDVHNADAVNYGAIGVVIGHEI
                     SHGFDDQGAQYDAQGRLNNWWTAEDLKRFHARTACVSDQFENYFIEPDIHHQGKLVLG
                     ESIGDLAGAKIAWLAFQKAQQTKPAVAIDGFTPEQQFFIAWGQFRGDETRPETQRLMV
                     QGDPHPVAKYRVIGPLSNLPEFAKTFGCKADAAMVRPADKRCAVW"
     gene            complement(2482..3033)
                     /locus_tag="VNN25_02145"
     CDS             complement(2482..3033)
                     /locus_tag="VNN25_02145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH90353.1"
                     /translation="MASAITPLILTIPGLWNSGPLHWQTHWEARHPSFRRVQQRDFDH
                     PDRDEWVATLDAAVRACPDPPILAAHSLGCSLVAQWAIDCGGRGVRGAFLVGPSDVEA
                     PDYPVEGRSFAKMPLAKLPFPSIVVASTNDQYVSSDRARLFADAWGSRLVFIGDAGHI
                     NGASGYGPWPEGERMLLDFSEEC"
     gene            complement(3312..4634)
                     /locus_tag="VNN25_02150"
     CDS             complement(3312..4634)
                     /locus_tag="VNN25_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXH90354.1"
                     /translation="MSTTEGPPPAGNDGLPLLGETLSFAKNPFGFMEERLARHGRIFR
                     SNVLGRKTAVIAGPEAAGKFIDTDLVKREGAMPPHVQELFGGRSLPLLDGEVHSVRKK
                     LVNQAFNRAAITAYLPIVQQTVERSFRAWPAAGEMRWLDEFKRLAIEVICTTVMGMQP
                     GAEMDRLREDYGTLTNGFASLPINIPGTRYRKALQARDRILDVLRRLVRERRQTPTDD
                     GLSRMLGAAITSNAELSDDDAALELHHIVIAGFIVFAELGSIVQQLTAHPDVRAKLTA
                     EIETVAPSGPLTLETLMAMPYLLQVVNEVKRLTPIVAAVFGKAKKSLEFEGVSVPAGW
                     MVMWAVTPSHVAQSSYTNPTAFDPDRFSPERAEDKRHEHAFAPQGAGPATGHRCPGLD
                     FATYIMEIFAVVLLRGYKWSIPPQNFEMDYSKTPPEPKDGLRATVTEA"
     gene            4804..5391
                     /locus_tag="VNN25_02155"
     CDS             4804..5391
                     /locus_tag="VNN25_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90355.1"
                     /translation="MTIRFRSVLVLLFAIASVQAASARTIVVPAGTSIHFKLRQTIST
                     ASAQTGQTVPATLTAPIVVNGVTVARTGSPAEVHISNAEASGRIGGSAKLTFSLASIT
                     LANGSVASVRTSRYSREGRAHAKHNAEYIAGAGIIGALAGQAIGHDSDATAKGAAIGS
                     GVGIAAAAKTGKFDFSLPAGSRFSLKLRAPIHATL"
     gene            5520..6677
                     /locus_tag="VNN25_02160"
     CDS             5520..6677
                     /locus_tag="VNN25_02160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90356.1"
                     /translation="MRVLVIASRYPWPAYSGDRMRTSSWLAALAPHARVALVAPEGMV
                     PDGVAPFDFYPARRSLTRGIRVAFTILSNGLPIQSLLAAPYDWDRAIAAARHDLGEFD
                     TTIVILARTDPWLRGSLEGGVKILDAIDSLRRNAAERGNAASIPMRWFWRQEERRLAR
                     AEKDIATFYDHVVVVSEEETSDLGAHAVAIGNGVPVAPLDIDAPRRFDFGFWGRLKYF
                     ANADAAMWLIDEIWPAILERNPSATCVIAGADAPRAILRAAERRAITFISPVDDVAAL
                     ARSVRVALLPIRYGSGQSNKVVEAGEAGCAIVATRQALRGLGPLASHVRFAHDASSFA
                     DAALALLVPHARYAAGSALRRVTSAMYSRSLVLDQLAHIAGVPVAAEAVTA"
     gene            6674..8026
                     /locus_tag="VNN25_02165"
     CDS             6674..8026
                     /locus_tag="VNN25_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXH90357.1"
                     /translation="MIRPSSKEKVIRVAGLMAGDFLLAWAALAIVVYIRRNVPLAFTK
                     SLLPPSTIRLDALTVCFFGGSLLIAFGLSGFYRRRALPHERPLIVTALLIQVAILAIG
                     GAFDQRLLPRTILLGVLAIEWLALPAWRRFVRIGLPIRPRQTILVGDAAQVRAAVAGL
                     DAVSDRRIRIAGWAGAPDQTLDETLSIPNIGLTSNPDVRVLLREIEEVIFVNPEASPR
                     DRLELLQIRGPRGYLLLASHADALLTSSMLGWVGDQPLIEIAVGCGYGLRAVVKRTMD
                     IVIGALLVVVAAPFALIAAAAIWLNDRGAVLIRQPRIGLGGVTFPMWKFRSMHVPHAG
                     RADGDGVTRIGGFLRRYRIDELPQLLNIIAGDMSLVGPRPERPEIVAQILSVLPEFEL
                     RSLVRPGLAGLAQVSAERDSRPEVKLRYDITYMCDWSLQLDVRLLLSSVSASLSGSGL
                     "
     gene            8071..9072
                     /locus_tag="VNN25_02170"
     CDS             8071..9072
                     /locus_tag="VNN25_02170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH90358.1"
                     /translation="MRETHLRTSATSADKTAARLLSIVIPTFNTAAMTLACCRAAIAA
                     APAESEVIVVDDGSTDGTCEVLRAEVPEVRIVRLDVNRRFAAAANAGVAAANGAIILL
                     LNSDTRVDREAPAKLFDAFAADVTLGVAGAQLIDADGTPQWSGGPQPTLLWMAVMVSG
                     IAHVLPRRKRTRGGDVGWVSGAAMAFRREAWNDAGPFNEMYRFYAQDLELCIRARAKG
                     WHVRVIEDARVVHDGGATLRRARHVAELPHDPSLLWLDLLSWGRAHYGRGWAWSARAL
                     MYTAASLRILSRTLRELFLRGDARRSSRSTTRVYAAALRQLFVEGEQSAGKDFGREA"
     gene            complement(8983..10287)
                     /locus_tag="VNN25_02175"
     CDS             complement(8983..10287)
                     /locus_tag="VNN25_02175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH90359.1"
                     /translation="MRILQLTPRLSYPPSDGGRVAMLQIATAMQRLGADVRILSLNPR
                     KQYADLETARQALAPIAVDAVDIDTSSLPASVLRGFLRIGTPMLVARFFSARFRRELR
                     ALLEASDVDVVQIESPFLLPYVPAIRAVSRATIVLRSLNVEFRIWEQIAEREKRVAFR
                     AGLQFIAGSLRRYEIRHLNTCDAIVPITEEDARDFRALGCSRPIHVLAGGVDVEEVSG
                     AEDIGGEDHVSGFARRRGRRRASRRDGGVPPAGMGNAAPRVGFLGSLDYRPNQDAAVW
                     IAEELGPRLRGKIHIAGSNAPQWLRERLDASGVTFEGEVPDAKAFIRSMDVMMAPLFS
                     GGGMRIKILEAMALGKPVVATTIGAAGIGVRHGEDILLADDVNSFAAAVSELLHDPAK
                     ARAIGDAGRRLVASRFSTEVLARGLLTFYEKLTKGRGVDAGR"
     gene            complement(10291..11586)
                     /locus_tag="VNN25_02180"
     CDS             complement(10291..11586)
                     /locus_tag="VNN25_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90360.1"
                     /translation="MIRTIGRVARSEGIGSAVRRAQERIGEAMRIQMMLARGAVGRPH
                     RSPILNISATGPFARLGGLPTQLIARLDEERALRDVAWLYPGIIELAVHARRAPQFTS
                     GDALFDAGFERSVAYALSVTGASAIHIEGTAGLPIGSILEMTKSGIDVVLGVHDFSLF
                     CARPHLIEEPMGVFCGYCMEADRCHRCLRETSNAGPNDQAQRRATARQLLAAARAVVF
                     PSTFLRDKHQELFALPDLNAHVIEPAVPDEPIARAVRRARGQRPRIAYAGSLKRHKGA
                     HFLPEIITAFADDDVDWHIFGGGDENFLRSVRHLPHTTVHGYYRGGTLPSLLARHQID
                     LGMLLSIWPETWCFTLSECWAAGVPAVGFAHGAIAERITSSGGGWLSPIEEGSYGIVN
                     IVRQWLAGELTTAIPAVTRAPRDAATDHGALYRSLNLLD"
     gene            complement(11583..>13561)
                     /locus_tag="VNN25_02185"
     CDS             complement(11583..>13561)
                     /locus_tag="VNN25_02185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90361.1"
                     /translation="LILVIDGPQDDAVETIVRSFASVVSQVLRNEARCGFVASVNRGM
                     RACSRDVVLLNSDTIVTARWLDKLIDASISSGDIGTVTPLSNNATLCSVPRVFEENLI
                     PTGFDAESFAALVERVSERTYPRLPTGVGVCLFIRRALLDDIGYFDVERFGLGYGEEN
                     DFCMRALARGWLHIADDATFIAHAGHRSFGSSSSALQRKAARTLARRHPDYMPAIAAF
                     MKMNPLASVHERINHALRARTPVTARRGPRKVVHLVHGWPPRQQAGTELYAYWLGRRQ
                     LQWREVSVFTRMADADRRQGEPVELSDDGARVRLVTNNFVQRNPVARNALRDWTFERA
                     FARFLAEEKPELIHIHHLAGHTFSLAGVAKRFGVPIVQQIQDWWPLCRRVNLFDFQWQ
                     RCSGPGIAKCAQCAPMTRIAPAPLWNRALHRMRRQAARRALSVADAFVMGSQCIHDDY
                     ERAGLFPAGILAFVLPYGVEMTAREARKPARRPLRFGIVGSIIPHKGIHIAADAFRGI
                     DPSLATLHVWGDTSASPAYVETLRALGGDLTFEGRFAEDAKAAIFDSIDVLLVPSVGL
                     ESFGLAAREAMARGVPVVASADGALLEMFEPGLCGELFPPRDADALQSIIVRLAIAPE
                     IVDGWSSRLPKVKSVDAHAEEIEKVYEIVMGSRR"
BASE COUNT         2518 a         4409 c         4216 g         2418 t
ORIGIN      
        1 atgacgccga tcgccgtcct cttcctcgcc acctcccttg ccgcgcagac cgctcccgag
       61 catggtgttt tcgtcgaaga catcgacaag aacgccgatg cctgcacgaa cttctacgac
      121 tacgcgaacg gtgcctggcg cgcggcgaat ccgattccag gctcgatgca gaagtggagc
      181 cgccgctgga aagccggcga gcagagcaaa gagcagctcc gtgcgctcct cgatgatgag
      241 tcgaagaaga aggactggcc gaagggatcg atcgatcagc agatcagcga cttctacgca
      301 tcctgcatgg atcaagcgcg cgtcgacgca cttggcgcta agccgatcca gccgctgttg
      361 cacgagatca aggcgatgcg caccgcggcg gatttgcaga agaccatcgc tgatcttcac
      421 gacctgcaga tgttcgcagc cttcggcatc acctcggcat ccgacaacca caatcccggc
      481 tacgtcatcg cccgcgtctt cgcctccggc ctcggcctcc ccgaccgcga ctactacctg
      541 aagcccgaga agcgcttcgc cgatgcccgc gccaagtacc gcgtccacgt cgcgaaaatg
      601 ttcgagctca ccggttactc ggcagccgag gcgaaacgcg ccagcgaagc gatcttcgcg
      661 atggagaccc ggctcgcggc cgcgcagctc gacaacgtcg cccttcgcga tcccgcggcg
      721 accgatcata aataccaggt ggccacgctg caaaagttca cgccacacgt cgactggacg
      781 cgctatctcg atcggttcaa agtcgcgcct gcggatctca acgtcgatca acccgaatac
      841 atgggcgcgg tcgagaagga gttgcgttcg acgccgctgg ccacatggaa ggcctacctc
      901 gcctggaatg tgatcaacag cgccgcgcgc catctctctt cgccgatcgc agccgaagaa
      961 tttgcgttct acggaaagta tctgagcggc tccacggaga tgaagccgcg ctggaagcgg
     1021 tgcgttgaag gcgatgacat gttcctcggc gaagcgctcg gccagcggta cgtggagcgc
     1081 tacttcccgc ccgaggccaa ggcgcggatg aaagagctgg tcaagaatct cctgctggcg
     1141 atgggtgaca ccattcgcgg tctcgactgg atgagtcccg ccacaaagac gcgcgcgctc
     1201 gagaaactgg ccaccttcaa tccgaaagtc ggttaccccg acaaatggaa ggactacagc
     1261 agcgtcgtga tcagcccaac gtcgtactgg gaaaacgtcg aggccggatt gaagttcgac
     1321 aacgcggaca gtctggcgca ggcgggcaaa ccggtcgatc gcggacgttg gggcatgacg
     1381 ccgccgacct ccgatgccta ctacaacccg ctgctcaacg agatcgtttt cccggccggc
     1441 atcctgcagc cgccggcctt cgacgtccac aacgccgatg ccgtgaacta cggcgcgatc
     1501 ggcgtcgtga tcggacacga gatcagtcac ggcttcgacg atcagggcgc gcagtacgac
     1561 gcgcagggcc gcctcaacaa ctggtggacc gccgaggatc tgaagcgctt ccatgcgcga
     1621 acggcgtgcg tgtccgatca gttcgaaaac tacttcatcg agcccgacat ccatcaccag
     1681 ggcaaactcg tcctcggcga atcgatcggc gatctggccg gcgcgaagat cgcctggctg
     1741 gcatttcaga aagcgcaaca gacaaagccc gcggtggcca tcgacggttt cacgcccgaa
     1801 caacagttct tcatcgcctg gggtcagttc cgcggcgacg aaacacgccc ggagacacag
     1861 cggctgatgg tgcagggcga tccgcatccg gtggccaaat accgcgtcat cggcccgctg
     1921 tcgaacctgc ccgagtttgc gaagaccttc ggatgcaaag ccgacgcggc gatggtccgg
     1981 ccagccgata agcgatgcgc ggtctggtga tcacgaggtt aaccggagcg tgatttcgtc
     2041 atcaacatca cagaaatcgt cttcaacgtt acagagattg gtgtccaacg atgcagagat
     2101 tgtctgtatc gataaagaca ttgtctgtat cgataaagac attgtctgca tcgatagaga
     2161 cgattgactg taacgttaga gacattgtct gcatcgacag agacgactga ctgtaacgtt
     2221 agagacattg tctgcatcga tagagacgac tgactctaac gttacagacg attgactgta
     2281 acgttagaga ccaatgtctt cagcgtcata gactttgtct tcaaggctaa cgtgattgtt
     2341 ttcagtgata tcgaacattc gcagaaccat cgaaacctgg atactgacga ttccttgacg
     2401 gtggcgttgc agcaggcaac gaacgtcatc atggccaatc gtctaggaca gcgagaacgg
     2461 ctacgagcga acacgctccg ctcaacactc ttcgctgaaa tcgagcaaca tccgctcccc
     2521 ttccggccac ggaccgtagc cggacgcgcc gttgatgtgg ccagcgtcgc cgatgaagac
     2581 gagacgactt ccccaggcat cggcgaagag acgcgcgcgg tcgctgctca cgtactgatc
     2641 gttcgtgctg gcgacgacga tgctcggaaa gggcagcttg gccagcggca tcttcgcgaa
     2701 gcttcgtcct tcgaccggat agtccggcgc ctcgacatcg ctcggcccga cgagaaacgc
     2761 tccccgcacg ccgcggccac cgcaatcgat cgcccattgc gcaacgagcg agcagccgag
     2821 ggagtgggcg gcgaggatgg gaggatcggg acaggcacgc acggccgcgt cgagcgtggc
     2881 cacccactca tcgcgatcgg gatgatcgaa atcgcgctgc tgcacgcgcc gaaaggacgg
     2941 atgcctcgct tcccaatgcg tctgccaatg caacggaccg gagttccaga ggccggggat
     3001 cgtgagaatg agaggggtga tagcgcttgc catcatcgga gtttagcccg cgagcttgct
     3061 gtcatcctga gactgccgga gccgcccgcg cgaatcgttg acgtttgcgc ggtgtgtgcg
     3121 cggcggcgta ggacgtcgaa ggatctcaaa ttacgaggcg catgccatgt gcgcggcaac
     3181 ttgagatcct tcgacgtcct gcggcgccgt cccacgatgc gtgagggagc gagtcgtgcg
     3241 ggcgcctccg gcgtctcagg atgacaccta acttcacggt tacgttaagg accattgggg
     3301 tgtcatcaaa ctcatgcctc cgtcaccgtc gcgcgcagac cgtccttcgg ttccggcggg
     3361 gtcttgctgt aatccatctc gaagttctgt ggcgggatcg accacttgta tccgcgaaga
     3421 agaactacgg cgaagatctc catgatgtat gtcgcgaaat cgagacccgg gcagcggtgt
     3481 cctgtcgccg gccccgcacc ctgcggcgcg aacgcatgct cgtgccgttt gtcctcagcg
     3541 cgctccggcg agaagcggtc gggatcgaat gccgtcggat tcgtataact gctctgcgcc
     3601 acatggctcg gcgtcaccgc ccacatcacc atccatccgg cggggacgga aacgccttcg
     3661 aattcgagcg acttctttgc cttgccgaat accgcggcca cgatcggcgt aaggcgcttc
     3721 acctcattga cgacctgcaa caggtacggc atcgccatca acgtctcgag cgtgagcggt
     3781 ccgctcggcg caacagtctc gatctcggca gtcagtttcg cgcgcacatc aggatgcgcc
     3841 gtgagctgct gcacaatcga tccgagctcc gcgaagacga tgaatccggc gatgacgatg
     3901 tgatgcagct cgagggcggc atcatcgtca gaaagctcgg cgttggatgt aatggcggca
     3961 cccagcatgc gcgacagtcc atcgtccgtc ggtgtctggc gccgctcgcg cacgagcctg
     4021 cgaagcacat cgagaatccg gtcgcgcgcc tgcaacgcct tgcggtaacg cgtgccggga
     4081 atgttgatcg gcagcgacgc gaatccgttc gtgagcgttc cgtagtcctc acgcagccgg
     4141 tccatctcgg ctcccggctg catccccatc accgtcgtgc agatcacttc gatcgccagg
     4201 cgtttgaact catccagcca gcgcatctcc cctgccgccg gccacgcgcg aaaggaccgc
     4261 tccacagtct gctggacgat cggcagatac gcggtgatgg cggcgcggtt gaaggcctga
     4321 ttgacgagct tcttccgaac gctgtgaacc tcgccgtcga gcagtggaag gcttcggccg
     4381 ccgaacagtt cctgtacatg cggcggcatc gccccttcgc gcttgacgag atccgtgtcg
     4441 atgaacttgc ccgccgcttc ggggccggca atgacggcgg tctttcgccc gagaacattg
     4501 gatcggaaga tccttccatg ccgcgccagc cgctcttcca tgaagccaaa cggattcttc
     4561 gcaaacgaca aggtctcgcc gaggagaggc agaccatcat tccctgcagg gggcggacct
     4621 tcagtcgtgc tcaatgggga cctccgatgt ttggtaacga tacgctgatg cagggccgct
     4681 ggatgttatt gatccgcaga tgacgcagat gatcacagat gaagcgcggc accttcatct
     4741 gcgtcccatc tgcgtcatct gcggatgaat ccggcatcgt cctcgcaagc aggagtcaaa
     4801 tacatgacaa tccgattccg ctctgtactc gttctccttt tcgccatcgc atcggtccag
     4861 gctgcgtccg cgcgcacgat cgtcgttcct gccgggacgt cgattcactt caagcttcgc
     4921 cagacgatct ccaccgcttc ggcgcaaacc gggcagacgg ttccggccac gctcaccgcg
     4981 ccgatcgtcg tcaacggcgt gacggttgcg agaaccgggt cgcccgctga agtccacatc
     5041 agcaatgccg aagcctccgg ccgcatcggc ggttcggcga agctgacgtt ctcgcttgcg
     5101 tcgatcacgc tcgccaatgg cagcgtcgcc agcgttcgca cgtcgcgcta ctcacgcgaa
     5161 gggcgcgcgc acgccaagca caatgccgaa tacatcgcag gcgccggcat catcggcgcg
     5221 ctcgccggac aagccatcgg ccacgacagc gatgcaacgg cgaaaggcgc cgcgatcgga
     5281 tccggtgtcg gaatcgctgc cgcagcaaag accggcaagt tcgacttttc cctccccgcc
     5341 ggaagccggt tcagtttgaa gttgcgagct ccgatacacg cgacgttgtg atccagaagc
     5401 cttaaccaca gagacacaga ggtcacagag aaatctctgt gatctctgcg cctcgggcgt
     5461 aaactctcat cactcagggc cctcctgccc agacggaggc agcccacgtc caaatcctca
     5521 tgagggtgtt agtgatcgct tcgcgctacc cgtggcctgc ctactcgggc gaccggatgc
     5581 gtacgtcgag ctggctcgca gcgctggctc cgcacgcgcg cgttgcgctt gtggcccccg
     5641 aggggatggt gccagacggt gtggctccgt tcgatttcta tcccgcgcgg cgctccttga
     5701 cgcgcggcat tcgcgttgcc ttcactattc tgagtaacgg tctgccgata cagagtcttc
     5761 tggccgcacc ctacgattgg gatcgcgcca tcgccgccgc ccgtcacgac cttggcgagt
     5821 tcgacacgac catcgtcatc ctcgcgcgca ccgatccctg gctccgcggc tcgctcgaag
     5881 gcggcgtgaa aatcctcgac gccatcgatt ctctccgccg caatgccgca gagcggggca
     5941 atgccgcatc gattccgatg cgatggttct ggcgtcagga agagcgtcgc ctggcgcggg
     6001 cggaaaaaga tatcgcgacg ttctacgacc acgtcgtcgt cgtgagcgaa gaagagacat
     6061 ccgacctcgg cgcccacgcc gtcgcgatcg gaaacggcgt tcccgttgcg cctctggaca
     6121 tcgacgctcc tcgacgattc gatttcggtt tctggggccg gctcaagtac ttcgcgaacg
     6181 ccgacgcggc gatgtggctg atcgatgaga tctggccggc catcctcgag cggaatccgt
     6241 ctgccacgtg cgtcatcgcc ggcgccgacg cgccgcgcgc gatcctccgt gcggccgagc
     6301 ggcgcgccat cacgttcatc tcgccggtcg atgacgtggc cgcccttgcg cgctccgttc
     6361 gcgttgcgct gcttcccatc cgttacggca gcggccagtc gaacaaggtc gtcgaagcgg
     6421 gcgaagcagg ctgcgcgatc gttgccacgc gtcaggcgtt gcgtggactc gggccgctcg
     6481 cgtcacacgt tcgcttcgcg cacgatgctt catcgttcgc agatgccgcg ctggcgttgc
     6541 tcgttccgca cgctcgctac gccgccggca gcgcgctccg ccgtgtcacc agcgcgatgt
     6601 actcgcgttc cctggttctc gaccaactcg cacacatcgc gggagtacct gtggccgccg
     6661 aggcggtgac ggcgtgatcc gaccgagctc gaaagagaag gtgatccgag tcgcgggcct
     6721 gatggccggc gacttccttc tggcgtgggc cgcacttgcc atcgtggtct acattcgccg
     6781 gaacgtacct ctcgccttca ccaaatcgct gctgcctcct agcaccatcc ggctcgatgc
     6841 gctgaccgtt tgcttcttcg gcggctcgct actgatcgca ttcggtctga gcggcttcta
     6901 ccggcggcgg gccctccccc atgaacgtcc gctcatcgtg acggcgcttc tcatccaggt
     6961 ggcgatcctg gcgatcggcg gcgccttcga ccagcgcctt cttcctcgca cgattctcct
     7021 cggcgtcctt gcgatcgaat ggctggctct ccctgcgtgg cgcaggttcg tcaggattgg
     7081 gctgccgatt cgtccgcgcc agacgatcct ggtcggcgat gccgcgcagg tgcgcgccgc
     7141 ggtggccggg ctggacgcgg tatccgaccg gcggatccgc atcgccggat gggccggggc
     7201 tcctgaccag acgctggacg agacgctgtc gattccgaac atcgggctga cgtccaatcc
     7261 cgacgtacgt gtgctgctgc gcgagatcga ggaagtgatc ttcgtcaatc ccgaggcgtc
     7321 gccgcgcgac agactggagc tgttgcagat tcgcgggcca cgcggctacc tcctgctggc
     7381 atcacacgcc gacgcgctcc tcacctcttc gatgcttggg tgggtcggcg atcagccgct
     7441 gatcgagatc gcggtcggct gtggttacgg actccgcgcg gtggtcaagc gaactatgga
     7501 tatcgtgatc ggagcgctgc tcgtcgtcgt tgccgcaccg tttgcgctca tcgccgccgc
     7561 ggcgatctgg ctcaacgacc gcggagccgt cctcatccgg caaccgcgca tcgggttggg
     7621 cggtgtgacg tttccgatgt ggaagttccg ctcgatgcat gtgccgcacg ccggccgcgc
     7681 cgatggcgac ggcgtcacgc gcatcggcgg attcctgcgc cgctatcgca tcgacgagtt
     7741 gccgcagttg ctgaacatca tcgccggaga catgagcctg gtcggcccgc gaccggagcg
     7801 gccggagatc gtcgcgcaga ttctcagcgt gcttccggag ttcgagttgc gcagtctggt
     7861 gcgtccgggc ctggcgggcc tggcacaggt ttcggcggag cgcgacagcc gcccggaagt
     7921 gaagctccgc tacgacatca cctatatgtg cgactggtcg ctacaactcg atgtgcggct
     7981 gttgctctcg agcgtgtctg cgtcgctctc cggaagcggc ctttgatccg cagatggctt
     8041 tgatccgcag atggcgcaga tgagacgcag atgagagaaa cgcatctgcg aacatctgcg
     8101 acatctgcgg ataagacagc ggcgcgacta ctctccatcg tcattccgac gttcaatacg
     8161 gcggcgatga cgctggcgtg ttgccgcgcc gcgatcgcag ccgcgccggc cgaaagcgaa
     8221 gtgatcgtcg tcgacgacgg ctcgaccgat gggacgtgcg aggtcttgcg tgccgaggtt
     8281 ccggaggttc ggatcgttcg gcttgatgtc aatcgtcgtt tcgccgcggc cgcaaacgcg
     8341 ggtgtcgcgg cggcgaacgg cgcgatcatt cttttgctca acagcgacac gcgagtcgat
     8401 cgggaagcgc cggcgaagct cttcgacgcc tttgctgccg acgtgacgct cggcgtggcg
     8461 ggcgcgcaac tgatcgatgc cgatggcacg ccgcagtgga gtggcggtcc tcaaccgacg
     8521 ctgctctgga tggctgtgat ggtgagcggg atcgcacacg tgcttccgcg gcgcaagcgc
     8581 acgcgcggcg gcgacgttgg atgggtgagc ggcgcggcga tggcgtttcg gcgcgaagcg
     8641 tggaatgacg ccggcccttt caacgagatg tatcgctttt atgcgcagga cctcgagctc
     8701 tgcattcgcg ctcgcgcaaa gggctggcac gtgcgcgtga tcgaggatgc gcgggtcgta
     8761 cacgacggcg gcgcgacgct gcgccgcgcc cgtcacgttg cggagcttcc tcacgatccg
     8821 tcgctgctct ggctcgacct gctgtcgtgg gggcgcgcgc attacggacg cggctgggcg
     8881 tggtccgcgc gcgcgctgat gtacacagcg gcgtcactgc gaatcctcag caggactctt
     8941 cgcgagctgt tcctgcgagg tgatgcgcgg cgatcgtcgc ggtcaacgac ccgcgtctac
     9001 gccgcggccc ttcgtcagct tttcgtagaa ggtgagcagt ccgcgggcaa ggacttcggt
     9061 cgagaagcgt gatgccacga gacgccgtcc cgcgtctccg atcgcgcgcg ccttcgccgg
     9121 atcatgcagc agctccgaaa cagccgcggc gaaggaattg acatcgtcgg caagtaggat
     9181 gtcttcgccg tgcctcacgc cgatccccgc cgctccgatc gtcgtcgcca ccaccggctt
     9241 gccgagcgcc atcgcctcca gaatcttgat acgcatgccg ccgcctgaga acagcggagc
     9301 catcatcacg tccatcgatc ggatgaacgc cttcgcgtcc gggacctcgc cttcgaacgt
     9361 gacgccgctc gcatccagcc gctcgcgcag ccattgcggc gcgttgcttc ccgcgatgtg
     9421 aattttgcca cgcagccgcg gccccaactc ctcggcgatc caaaccgcag catcctgatt
     9481 gggccggtag tcgagcgagc cgagaaaccc gacgcgcggc gcagcgttgc ccatacccgc
     9541 gggcggaacg ccgccgtctc ggcggctggc ccggcggcgt cctcgccgcc gcgcgaatcc
     9601 ggaaacgtga tcctcgccgc caatgtcctc ggcgcctgac acctcctcca catccacacc
     9661 gcccgcaaga acatgaatag gccgcgagca cccaagcgcc cgaaagtcgc gggcgtcctc
     9721 ctccgtaatc gggacgatgg catcgcacgt gttgaggtgg cggatctcgt aacggcgaag
     9781 gctgccagca atgaactgca atcccgctcg gaacgccaca cgcttttcac gctccgcaat
     9841 ctgctcccag atacgaaact cgacgttgag cgatcgcagg acgatcgtcg cacgcgacac
     9901 ggcgcgaatc gccggaacat acggcagcag aaacggactc tcgatctgaa cgacatctac
     9961 atcggacgcc tccagcaacg ctcgcaactc tcgcctgaac ctcgccgaga agaagcgggc
    10021 cacgagcatc ggtgtcccga tccgcaggaa accgcgcagc accgaagcag gcaagctgga
    10081 ggtgtcgatg tcgactgcgt cgaccgcaat cggagcaagc gcctgccgtg ctgtctccag
    10141 atccgcatac tgctttctcg gattcagcga caggatgcgc acatccgcac cgagtcgctg
    10201 catggccgta gcgatctgaa gcatggcgac gcggccaccg tcgctcggcg gatacgagag
    10261 tcgcggcgtg agttggagga tgcgcatcgt ttagtcgagc aagttcagcg aacgataaag
    10321 cgcgccgtga tcggttgctg cgtcccgagg cgctctggta actgcaggaa tggccgtcgt
    10381 caactcgccg gccagccatt gccgcacgat gttcacgatg ccgtacgaac cttcctcgat
    10441 cggcgaaagc cagccgccgc cgctgctcgt gatgcgttca gcgattgcgc catgggcgaa
    10501 tccaacggct ggcacgccgg cggcccagca ctcggagagc gtgaaacacc acgtctcggg
    10561 ccagatcgaa agcaacatcc cgaggtcgat ttggtgcctg gcgagaagcg agggaagcgt
    10621 gcctccgcgg taatagccgt ggaccgtcgt gtgcggaaga tgccgtacgg accgcaggaa
    10681 gttctcatcg ccgcctccaa agatgtgcca gtcgacatcg tcgtcggcga aagcagtgat
    10741 gatttccggc aggaagtgag cacccttgtg tcgtttcagg ctgccggcat atgcgattcg
    10801 cggccgttgt ccgcgcgctc tgcgcacggc tcgcgcgatc ggctcgtcag gcactgctgg
    10861 ctcgatcacg tgcgcgttga gatccggaag cgcgaagagc tcctggtgct tgtctcgcag
    10921 aaatgtggac gggaagacga cagcacgcgc agctgccagc agttgccggg ccgttgcgcg
    10981 gcgctgcgcc tgatcattcg ggccggcgtt cgaggtttcg cgcaggcaac ggtgacatcg
    11041 atccgcctcc atgcaatagc cgcagaaaac gcccatcggt tcttcgatca gatggggtct
    11101 ggcgcagaaa agggaaaaat cgtgcacgcc gagaacgacg tcgatccccg atttcgtcat
    11161 ctcgaggatg ctgccgatcg gaagacccgc cgtcccctcg atgtggatcg cgctcgcgcc
    11221 ggtcacgctc agcgcgtacg cgacggaacg ctcaaatccc gcatcgaaca gcgcatcgcc
    11281 ggatgtgaat tgcggcgcgc ggcgggcgtg cactgccaat tcgatgatcc ccggatagag
    11341 ccaggccaca tcacgcaagg cgcgctcctc atcgaggcga gcgatgagct gcgtcggcag
    11401 cccgccgagg cgcgcgaacg gtccggtcgc ggagatgttc aggatcggcg atcggtgtgg
    11461 acgaccgact gctcctcggg cgagcatcat ctggatgcgc atcgcctcgc cgatccgctc
    11521 ctgcgcgcgg cgaaccgcgg aaccgatgcc ttcggagcga gccacgcggc cgatcgttcg
    11581 aatcatcggc gcgagcccat cacgatctcg tagaccttct cgatctcctc cgcatgcgcg
    11641 tcgacgctct tcaccttcgg aaggcgtgac gaccagccgt cgacgatctc aggggcaatg
    11701 gccagccgga cgatgatgct ttgaagcgcg tcggcatcac gcggcggaaa cagctctccg
    11761 cacaatcccg gctcgaacat ctcgagcaga gctccgtcag ccgaggccac gaccggaaca
    11821 ccgcgggcca tggcctcgcg cgcggcaagg ccgaacgatt cgaggccgac cgaggggacg
    11881 agcagcacat cgatcgagtc gaagatcgcc gccttcgcat cctcggcaaa ccttccttcg
    11941 aaggtcagat cgccgccgag cgctcgcagc gtttcgacgt acgccggcga agcggacgtg
    12001 tcgccccata cgtgcaacgt ggccagcgac ggatcgatcc cgcgaaatgc gtccgcggcg
    12061 atatgaatgc ccttgtgagg aatgatcgat ccgacgattc cgaaacggag cgggcgtcga
    12121 gcgggtttgc gcgcttcccg cgctgtcatt tccacgccgt acggcaacac gaacgccaga
    12181 atccccgcgg gaaacaaccc ggcgcgttcg taatcgtcgt gaatgcactg cgaccccatt
    12241 acgaaggcgt cggccaccga aagcgctcga cgcgccgcct gccgccgcat gcgatgcagc
    12301 gcgcggttcc agagcggagc gggagcgatg cgcgtcattg gcgcgcactg cgcgcacttg
    12361 gcgatgcccg gcccgctgca cctctgccat tggaaatcaa acaggttcac gcgtctgcac
    12421 agcggccacc aatcctgaat ctgctgaacg atcggaacgc caaagcgttt cgcgacgccg
    12481 gccagcgaga aagtgtgccc ggccagatga tggatgtgga tcagctccgg cttttcctcg
    12541 gccaggaacc gcgcgaaggc gcgctcgaac gtccagtcgc gcaacgcatt gcgcgcgacc
    12601 ggattgcgct gcacgaagtt gttggtcacg agccggacgc gcgcgccgtc gtcagagagt
    12661 tcgacgggct cgccctgtct gcggtctgcg tcggccattc gcgtgaagac cgagacctcg
    12721 cgccactgca gttgccgccg accgagccaa tacgcataca gctccgttcc ggcctgctgc
    12781 cgcggcggcc agccatgcac gagatgaacg actttgcgcg gcccgcggcg tgccgtaacc
    12841 ggcgtgcggg cgcgcaacgc gtgattgatg cgctcatgca cggaagcgag cggattcatc
    12901 ttcatgaacg ctgcaatcgc gggcatgtag tcaggatgcc ggcgcgccaa tgtcctcgcc
    12961 gccttgcgtt gcagtgcgct gctcgacgag ccgaagctgc ggtggcccgc gtgcgcgatg
    13021 aacgtggcgt cgtccgcgat gtggagccac ccgcgcgcca gcgcccgcat gcagaagtcg
    13081 ttctcttcgc cgtagccgag gccgaaacgt tcgacatcga agtacccgat gtcgtcgagc
    13141 agagcacggc ggatgaagag acagactccg acgccagtcg gcaaccgcgg atacgtccgc
    13201 tcagaaacgc gctcgacgag cgccgcgaat gactccgcgt cgaagccggt ggggatgaga
    13261 ttttcttcga atactcgcgg cacggagcag agcgtggcgt tgttcgacag cggcgtgacc
    13321 gtcccgatgt cccccgagga gatgctggcg tcgatcagtt tgtcgagcca gcgggcggtg
    13381 acgatcgtgt cgctattgag caggacgacg tcgcgcgagc atgcacgcat cccgcgattc
    13441 acactggcga caaatccgca gcgcgcttcg ttgcgcagga cttgcgaaac caccgaggcg
    13501 aacgagcgca cgatcgtttc gacggcatca tcctgcgggc cgtcgatcac cagaatgagt
    13561 c
//
LOCUS       DATLVW010000065         8205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_374517, whole genome shotgun sequence.
ACCESSION   DATLVW010000065 DATLVW010000000
VERSION     DATLVW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374517"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1954)
                     /locus_tag="VNN25_02190"
     CDS             complement(125..1954)
                     /locus_tag="VNN25_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXH90362.1"
                     /translation="MTIEQEGRLAKPPLALAERAILLVLAAGLLVVPVILGSGLDTFR
                     LPKELIFRGEAIALLMLAVFWLTSRRRTWTFTRRPEFLLTAAVIGWCLITTATSTNRP
                     LSVDSLITILAAAVIFIATCLAAETVTVIAIDVLMIACCANAVLVILQELRIWTPFPL
                     PPDTAAHYGSVALLGNANDVGMYLVVPAIAAIVLAVTASGLRRWIYAGVGILLVAGIA
                     ASGTRTALGALLAALIVFALSHSRRAALVVGAIIILLGLVILSPSTSMGRGVRELAEA
                     VKHRDYQNLFSERLPPFLSALDMARDHPLAGVGPGCFKYHYMAYRIGLRGRYPDPWLE
                     GFAMNWGEVHNDHLQVAAETGLPGYALFLLAIGIGSGIVGSLRRAPVSASTPEATFAR
                     AFRWPLATALFVICLAQFPLEIAAPRLVILTLGALCVTWSNDDRAPRPAGAHPPRLAQ
                     RPFAALLLRMAALVAAGYAVNQLCIAPYRGNLILREVAARSLHAQSLDDRHAIDIAHT
                     NLHDLDEAARGRRLDPAWYLLYGTNCEILGRWDEAAATYTTALRIDDRPEIYVNRGLV
                     RLHLGQTDLAVSDLATAARFDPNVIYQLEGELRARVTAAVGAR"
     gene            2244..2789
                     /locus_tag="VNN25_02195"
     CDS             2244..2789
                     /locus_tag="VNN25_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH90363.1"
                     /translation="MSQSTYEDYLALEHDSELKHEFADGEIFAMAGGSPRHSALAFRI
                     SVALGATLPPGCTGFQSDMRIRVLATGRATYPDVSMVCGPIELDPDDRSGTTITNPLL
                     LVEVLSPSTEDVDRVSKWRDYQRIPSLQEYVLVNQEPRVEIYRRLASGSWEYLDVQEG
                     VVKLVSGATLDLSILYANLPN"
     gene            3883..4425
                     /locus_tag="VNN25_02200"
     CDS             3883..4425
                     /locus_tag="VNN25_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90364.1"
                     /translation="MSNATPVTPNPDPAKLAPEQVIAQLRTMSSQMEDVAPLSKEQRK
                     LIRQRLRIQPKPIVEASINVIGVLDNVSQAIGQPLDEVRQLQDDSLRWDAVAEEARAF
                     LKGIEGANLNRRQRLSLIGMQAYAIGTQLAKDPDNAVLVPHVEEVKRLKGVSRRKKVK
                     QAPPTPAPAPAPVTSTASKS"
     gene            4487..5545
                     /locus_tag="VNN25_02205"
     CDS             4487..5545
                     /locus_tag="VNN25_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90365.1"
                     /translation="MKNDYASQRAAVLIDETIAALQRMEDAPTSPLKGFRTTKRRREY
                     RRYAERLRRGQVEPVYKNLHTAEQLAEIYEQTARRDEIFEEACGDLARISRDLKRVVE
                     QEGSAAAIRTFNAMLREAGLAAAFNGPASEAAQRHRRMWFLISVGARRESEKRRQLPH
                     QPFLPLLTKDPRTDLRDELAAAVILDSPPTGEAVIAIPAEDSESGCGRVLMRIGVGNA
                     SWIGSFERGQKGPGPSTVQMMPDGKHLFVSAAGAGYIIDATSRTLVEQTGTDVVSVEG
                     NEPGTLFVINHNDMSLEAFGRAGRLWKTDAISSGGLRRMTASDDAIIGEARRPSPPGW
                     AAFSVELATGEVRFGEAR"
     gene            complement(5586..6734)
                     /locus_tag="VNN25_02210"
     CDS             complement(5586..6734)
                     /locus_tag="VNN25_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXH90366.1"
                     /translation="MLVVAVTIVVVSANAADFKISSAEDVTRAKKEWDARVRFERAVD
                     PILKDVSLEELTLISASTAAAITRVQSEIENRSRLKKAIDPILRKYTIEELSIILSQA
                     SIHHGDTTPVLQAKPATPQSVINRDATVALIPISLLKKQADGTYQLNTSTTVGAAFAM
                     CAREQFFNEPSAASCAGVLIAPDLVITAGHCVFNAQYLDLAVVFGYRVDGSGQLPAFY
                     KADDVRFLTDETDVTNVIFGQDWGIVTLDHAVTVSPARIRMSGVVGDQDGVYVIGYPA
                     GMPESAAGIATANVKDNRDNDVFMADIDTLERSSGSPVFNRNDDTVEGIFEHGDKWVN
                     TGTCNLIMRCPASGICADEEVTRIRCLPLAPDVRGVLKISGKTSQPCK"
     gene            complement(6758..7549)
                     /locus_tag="VNN25_02215"
     CDS             complement(6758..7549)
                     /locus_tag="VNN25_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90367.1"
                     /translation="MRIEFEGGICEVMAHKSGTPRAVFLVNKMTPHLVEIFLPLNPDV
                     TPAAQKKEIEDVTGLTAQPISTKGFRLSNVKGWMFRIVDPTGSGSPMQPALTPSLDFD
                     FLVPHLSRVAGVTEADLDPGIDKDPDASGKFVDFVELDGGVLSAVPYCKRGVFDPDTE
                     KRSIRAFAQTVVLTGSTAATAVLQMRKTSRDTWQSLHFGSVDLVRITFRNQPASSDGM
                     PHFDMHKNILNSGSTLTWPDIPPGQIVEFCPQSDTVPGCSDTQWP"
     gene            complement(7688..>8205)
                     /locus_tag="VNN25_02220"
     CDS             complement(7688..>8205)
                     /locus_tag="VNN25_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH90368.1"
                     /translation="ASCYRGASILRGYEVTHSRFIDEIRAANIVHFAGHALNVPDRPL
                     DSALIVAPDKGVASVAASDIMQLHLNHILLVFLSTCRSAESGGRRDGVQNLATAFLVA
                     GAPTIVASISDVEDQSARRLATAFHSKFATSDDAASALHEAAVQSGDYREHPSTFRMY
                     VLGGSRRFVGH"
BASE COUNT         1667 a         2540 c         2510 g         1488 t
ORIGIN      
        1 agcagcgttt cgaagccgtc ggccggaagc gcctcgctca accagaccac gtgccgggcc
       61 ggaccgccta cgttgagccg ggcgatgagg cgaaggacgc ggattggccg cggcgcatcg
      121 ggcgtcatcg cgcgccgacc gcggcggtta cgcgggcgcg aagctcaccc tcgagttggt
      181 agatgacatt cggatcgaaa cgtgcggccg tggcgagatc ggatacggcg aggtccgttt
      241 gtcccaggtg cagtctgacg agaccgcggt tcacgtagat ctccgggcgg tcatcgatgc
      301 gcaaggcggt cgtgtaggta gccgcggcct cgtcccagcg gccgaggatc tcgcagttgg
      361 tgccgtagag cagataccag gcgggatcga ggcggcgccc acgggcagct tcgtcgagat
      421 cgtgcagatt cgtgtgggca atgtcgatgg cgtgccggtc atcgagtgat tgcgcgtgca
      481 gcgaccgcgc cgcaacctcc cgcaggatga gattcccgcg gtagggagcg atgcatagct
      541 ggttgacggc gtagccggca gcgacgagcg ccgccattcg cagcagcagc gctgcgaagg
      601 gtctttgcgc cagccgaggc ggatgcgcgc ccgcggggcg aggcgcccga tcgtcattgc
      661 tccatgtgac gcagagtgct ccgagcgtga ggatgaccag ccgcggtgcc gcgatctcga
      721 gcggaaattg ggcgaggcag atcacaaata gtgccgtcgc cagcggccag cggaacgctc
      781 gcgcaaacgt tgcttcaggc gttgatgcgc tgacaggcgc gcgtcgcagg cttcccacga
      841 ttccactgcc gatcccgatc gccaggagga agagcgcgta tcccggcagt ccggtctcgg
      901 cagccacctg caggtgatcg ttgtggacct cgccccagtt catggcgaag ccttccaacc
      961 acggatcggg atatcgcccg cgaagcccga tgcggtaggc catgtagtga tacttgaaac
     1021 agcccggtcc gactccggcg agcggatgat cgcgcgccat gtcgagcgcg gagagaaacg
     1081 gaggcagacg ctccgagaat agattctgat agtcgcgatg cttcaccgcc tcggcaagct
     1141 cgcgaactcc tcggcccatc gaagtcgacg gagaaagaat caccagcccg agcaggatga
     1201 tgatcgcgcc aacgaccagc gcggcacgcc gggagtgact gagcgcgaag acgatcagcg
     1261 cggccagaag cgccccgagg gcggtgcgtg tgccgctggc ggcgattccg gccacgagca
     1321 gaatccccac tcctgcgtag atccagcgcc ggagaccgct cgcggtcaca gcgagcacga
     1381 tcgccgcgat ggccgggacg acgagataca tgccgacgtc gttggcattg ccgagcagcg
     1441 caacactgcc gtagtgcgcg gctgtgtccg gcggcagagg aaacggcgtc cagatcctca
     1501 gctcctgcag gatgacgagg accgcattcg cgcagcacgc gatcatcagc acatcgatgg
     1561 cgattaccgt caccgtctcc gcggccaggc aggtcgcaat gaagatgact gcagcagcaa
     1621 gaatggtgat caggctgtcg acgctgagcg gtcgattcgt cgatgtagct gtcgtgatca
     1681 gacaccagcc gatgaccgcg gccgtgagca gaaactcagg gcgccgcgtg aacgtccacg
     1741 tgcgccgccg tgacgtcagc cagaacacgg ccagcatcag gagcgcgatc gcctcgccgc
     1801 ggaagatcaa ctccttcggc aggcgaaacg tatcgagccc actgccgaga atcaccggca
     1861 cgacgagcag acccgcggcg agcacgagaa gaatggcccg ttcggcgagg gcgagcggcg
     1921 gcttcgccag ccggccttcc tgctcgatcg tcatcacggc gccccctctt cgaacccatg
     1981 ttggcgggcg gacgcccagg cgcggttcgt gatgctcatg tgtgatgggc tcgccggaaa
     2041 tctaggcgcg ccgattctgc cacatcggag tgcggtcgtc tcgaccgcgg gccctcgggc
     2101 gtcctcgccc gagggctgtc agggcggagg tcgacgtcca ggagtgaatg accgagtttt
     2161 ctaggggtac cttgcaatcg ccagactgac aactctcctg gcggtcgatt accctgaaca
     2221 cgcgacaacc acacctctgt ctcatgagcc aatccaccta tgaggactac ctcgcgctcg
     2281 aacacgacag tgagctgaaa cacgagttcg ctgacggtga gatctttgcc atggcgggcg
     2341 gatcgcctcg gcacagcgcc ctcgccttca ggatcagcgt tgccctcggg gccacccttc
     2401 ctccgggttg cactggattc cagtcggata tgcgaatccg cgttctcgcc acgggccgag
     2461 ccacctatcc ggacgtctcg atggtctgcg gtccgatcga gctcgacccc gatgaccgca
     2521 gcggcacgac gatcacgaat cccctgctac tcgtcgaggt cctatccccg tcgaccgaag
     2581 acgtcgaccg ggtgagcaag tggcgggatt accaacgcat cccgtcgctg caggaatacg
     2641 tcctcgtcaa tcaggagccg cgcgtcgaga tctaccgccg tctcgcgtca ggaagctggg
     2701 agtatctcga cgtgcaggaa ggtgtcgtaa agctcgtgtc gggcgcgacg ctggaccttt
     2761 cgattctgta cgcaaacctt ccgaactgag cggctcctgc ctttcgtgtg cgcgtgacgg
     2821 ctcacggcgc accgtcaaca ctccacgttc ggtgcgcaat gcgtctcagt cgcttcatcc
     2881 tgccatttgt ggcgatcgtg tggcacgccg gacgacttgc ggtgcgcatc gggcgcacgc
     2941 gatcgacacc gtgtcgcaac gttggacccc cggtgcgcac gtacgacacc cactcgcgcc
     3001 gcaacgctac acctccggtg tagcatgcga caccgtctat gaagcaacgc tccacccctg
     3061 gtgtggcatg ccacaccgtc tatgaagcaa cgctccaccg ctgatgtggc atgcgacact
     3121 gcccatgaag caacgctaca cccccggtgt agcaatgcga cgccacccat gtagcaacgc
     3181 cacacctcgg gtgtagcaat gcgacactgc ccatgaagca acgctacacc cccggtgtag
     3241 ccaatgcgac accacccatg tagcaacgct acacctcggg tgtagcaatg caacactgcc
     3301 catgccgcaa cgctacactc ccggtgtcgc aatgcggcac tgcccatgta gcaacgctac
     3361 acgcccggtg tagcaatgcg gcactaccca tgtagcaacg ctacacgccc ggtgtaggaa
     3421 tgcgacgctg cccatgaagc aacgctacac ccccggtgta gcatgcggcc acggcccgtg
     3481 tagcaacgct acatccgccg tgtagcaacg ccacattctc ggttgcacat gtgcgcgcgc
     3541 atcgtgttgc acgatttttt ttgcgtgtag ccgcattgcc ccttgacaag tcgtttcaat
     3601 gggcttatat cgttcgctac aacgtcgaat ttcgtaagat cttcggcttt ggcggctgtc
     3661 ggcggtacag gatggtgctg gatagcacac gattaaacct gtaccggcgc gggagttgag
     3721 cttgctcctc ctgccgcggg cacccgaggt gtgtacacac cgcgggaaaa catcaaccgg
     3781 aagaatcggc gcgtagccgc gacttccagc cggtggccgc tcgcacttgt cgacgagtcg
     3841 tgacgtcacc tccggcctta gctcgtagaa ggagactatg tcgtgagtaa tgcaacccct
     3901 gttacaccaa accctgatcc ggccaagctg gcccccgaac aggtaatagc gcagttgcgc
     3961 accatgtcgt cgcagatgga ggacgtagcg ccgttgtcga aggagcagcg caagctgatc
     4021 agacagcgtc tgcggataca gccaaagccg atcgtcgagg catcaatcaa cgtcatcggc
     4081 gtgctggaca acgtatcgca ggcgatcggg cagccgctcg acgaagtccg tcagctgcag
     4141 gacgactcgc tccgttggga cgccgtggcc gaagaggcgc gcgcgttcct gaagggtatc
     4201 gagggagcaa acctcaatcg ccgtcaacgg ctctcgctca tcgggatgca ggcgtacgcg
     4261 atcgggacac agctcgccaa ggacccggac aacgcagtgc tcgtaccgca cgtcgaggag
     4321 gtcaaacggc tgaaaggcgt ctcgcgccgg aagaaggtga agcaagcccc tccgacgccg
     4381 gcgccggcac ccgcgcctgt aacgtccacc gcatcaaaat cgtaaacgta taaggaggta
     4441 gtgaacccgg gcgcgcacag aagcgcgccc gggttccatg acgatcatga agaacgacta
     4501 cgcatctcaa cgggccgcgg tgctgatcga cgagaccatc gccgccttgc agcgcatgga
     4561 agacgcaccg acctcaccct tgaagggctt ccggactacc aaaagaagac gggaataccg
     4621 gcgctacgcc gagcgcctgc gccgcggaca agtggagccg gtctacaaga atcttcacac
     4681 cgccgagcaa cttgcagaga tatacgagca gaccgcccgg cgcgacgaga ttttcgaaga
     4741 ggcgtgtggc gatttggcga gaatcagccg ggacctcaag cgcgtcgtcg agcaggaagg
     4801 ctctgccgcc gccataagaa cattcaacgc gatgctccgg gaggcggggc tcgcggcggc
     4861 gttcaacggt ccagccagcg aggccgcgca acgacatagg cgaatgtggt ttctcatctc
     4921 ggtcggcgcg aggcgcgaat ctgagaagcg ccgccaactg ccgcaccagc ccttcctccc
     4981 actcttgacg aaggatccgc gaaccgacct gcgcgatgag ttggccgcag ccgtgatcct
     5041 cgactcaccg cccacgggcg aagcggtgat cgcgattccg gccgaggatt ccgaatccgg
     5101 ctgcgggcgc gttctcatgc gcatcggtgt cggcaatgca tcatggatcg gcagcttcga
     5161 gcgtggtcag aagggtcccg gtcccagcac cgtccagatg atgccggacg gcaagcacct
     5221 gttcgtatct gcggccggcg ccggctacat catcgacgcg acgtcgcgaa cgctcgtaga
     5281 gcagaccggc acggacgtcg tcagcgtgga gggaaacgaa ccgggaacct tattcgtcat
     5341 caatcacaac gacatgagcc tggaagcgtt cggaagagcc ggccgcctct ggaaaaccga
     5401 cgccatcagc tccggaggac tccgacgaat gacggcctcg gacgacgcga tcatcggcga
     5461 agcgcggcga ccatccccgc ccggctgggc cgctttctcc gtggaactgg cgacgggcga
     5521 agttcgtttt ggcgaagcac gttgaagatc gggagagctt gggggtccta cggaaacagc
     5581 acgtcctatt tgcacggttg tgacgtctta ccactgatct tcaaaacgcc cctcacatct
     5641 ggggccagcg gaaggcaccg gatgcgtgtc acttcttcat cggcgcaaat cccgctcgca
     5701 gggcaacgca tgatgagatt gcaggtgccg gtgttcaccc atttatctcc gtgttcgaaa
     5761 atgccctcca ccgtgtcgtc attgcgatta aataccggtg accctgaact gcgctccagc
     5821 gtgtcaatgt ctgccatgaa gacatcattg tcgcggttgt cctttacatt cgctgtggcg
     5881 atacctgcag cggactccgg catgcctgct ggatatccga tcacatacac accgtcttgg
     5941 tctccgacga ctccgctcat tcgaatacgc gccgggctga cagtgacggc gtggtcgaga
     6001 gtgacgattc cccaatcctg tccgaagatc acgttcgtga cgtcggtctc atccgtgaga
     6061 aatcggacgt cgtccgcctt gtagaaagca ggcagttgac cgctgccgtc gactcgatat
     6121 ccgaaaacaa cggcaagatc gaggtactgc gcgttgaaca cgcagtgccc cgcggtgatc
     6181 acaagatcgg gagcgatgag gacgcctgca caggaggcgg cagacggctc gttgaagaac
     6241 tgctcgcggg cacacattgc gaaagcagcg ccgactgtcg tgctagtgtt gagctgatag
     6301 gtcccatccg cttgtttctt caacagagat atcggtatca gtgcaaccgt cgcatccctg
     6361 ttgatcacac tttgcggagt agccggtttt gcttgcaaaa cgggagtcgt atcaccatgg
     6421 tggatacttg cttgactcag gatgatcgag agctcttcga tcgtgtactt cctcaagatg
     6481 gggtcgatcg ctttcttgag ccggctgcgg ttttcaattt cgctctgtac gcgggtaatc
     6541 gccgcggccg ttgacgcgct gatcagcgtg agctcttcaa ggctgacgtc cttcaagatc
     6601 ggatcgactg ccctctcgaa tcgcacgcga gcgtcccact ctttctttgc tcgcgtgacg
     6661 tcctctgccg aactgatttt gaagtcagcg gcgttagcag atacgacgac gattgtgacc
     6721 gcgacaacca gcaagaatat cctcaaagcg cgcggcctca tggccactgc gtgtccgagc
     6781 agcccgggac tgtgtccgac tgaggacaaa actccacaat ctggcccggg gggatatcgg
     6841 gccaggtcaa cgtcgatccg ctgttcaaga tgttcttgtg catgtcgaaa tggggcatac
     6901 catccgatga cgccggttgg ttccggaacg ttatgcgtac caggtcgacg gacccaaagt
     6961 ggagtgactg ccacgtatca cggctagttt ttcgcatctg cagcacagcg gttgccgcgg
     7021 tggaacccgt gagaaccacc gtctgcgcaa acgcgcgaat cgagcgcttt tcggtgtcgg
     7081 ggtcgaaaac gcccctctta cagtacggca ccgcgctcaa cacgcctccg tccagttcaa
     7141 caaaatcgac aaatttcccg ctcgcgtcag gatctttgtc aatccctgga tcgagatcgg
     7201 cctctgtgac ccccgccacc ctggagaggt gaggcacgag aaaatcgaag tccaatgatg
     7261 gcgtaagggc tggttgcatc gggctgccgg atcctgtcgg gtcgacaatg cggaacatcc
     7321 accctttcac gttgctcaac cgaaatccct ttgtcgagat cggctgcgcc gtcagaccgg
     7381 tgacgtcttc gatctctttc ttctgggctg ccggcgtcac gtccggattc agcggcaaga
     7441 agatctcgac gagatgaggt gtcattttgt tgacgaggaa gacggcccgc ggagtgccgc
     7501 tcttgtgtgc catcacttca cagattccgc cctcgaactc gatccgcacg agtttcgccg
     7561 gagtgacgga ggcgggcttc gcgggcgtgc cgggcggtac cggccctttg cagcttgccg
     7621 cgatgacggg gaccagaacg agcatcattt ggcggacaaa aacggttttt accatttctc
     7681 tctcctctca atgtcccaca aagcgccggc tgcctccgag cacatacatt cgaaatgtag
     7741 atggatgttc tcgataatcg ccggactgca ctgccgcttc gtgtagtgcc gaggcagcgt
     7801 cgtcggatgt ggcgaacttg ctgtggaatg cggtcgcaag tcggcgggct gattgatcct
     7861 caacgtcgga gatcgaagcg acaatcgttg gcgcgccagc gaccagaaag gcagtcgcta
     7921 gattctgtac gccgtcccga cgtccgccgc tttccgcgga acgacacgtc gataagaaaa
     7981 caagcagtat gtgattcaga tgcaattgca tgatgtctga agcggcgact gaggcgacgc
     8041 ctttgtctgg cgcgacgata agcgccgaat cgagcgggcg gtcgggaaca ttcagggcgt
     8101 gcccggcgaa gtgaacgatg ttcgccgcac ggatctcgtc gataaacctg ctatgcgtga
     8161 cctcgtatcc gcgaaggatg ctggcgccgc ggtaacaact tgcca
//
LOCUS       DATLVW010000066         9868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_169168, whole genome shotgun sequence.
ACCESSION   DATLVW010000066 DATLVW010000000
VERSION     DATLVW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169168"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VNN25_02225"
     CDS             <1..350
                     /locus_tag="VNN25_02225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH90369.1"
                     /translation="RFGESRNIRKASFGKLASARLRRPFDLVVCSDALHYVPDDEIHA
                     GLPELVRLAGGVLFLEVLTADDDIIGDLNGMIRRPAIWYRKLFEKAGLLQVAPYTWLA
                     PALRSEAAELEKS"
     gene            complement(416..1795)
                     /locus_tag="VNN25_02230"
     CDS             complement(416..1795)
                     /locus_tag="VNN25_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH90370.1"
                     /translation="MGVDRLVIRRLSLLVLLISTSALAQTVQITSKPTALVYGNLFVP
                     VQTHEPVVRVALFINGVKFDEKPGGSVDFPVVIGDYLRRLRFRAVGYDAQGNVAGEDE
                     MVVNDPRPPFRIRLHAGPGRLEANVVKPEQLAISSVDFLIGEEKVASASAPPYNAVFD
                     PAKFPNAVYARVVAHARDGSEANDVLFFGTNPSEQVDVAVQQVPVSVVGGNRAPKAGE
                     LTLLDNGVPRKIEALTPASDQPLYVILLIDYSESMLEELPVVKLAARQFAQKLLRPQD
                     RIAVVGFNQTTFWLTPFTSDFNAAAASVDRVKPSGETHLYDTAIEMLYELQKQPGRHA
                     LVILTDGVDQGSHFKLDHLVHYARYAGVPVYPIIKNKILTRLMRFGVGYLEARKLAAI
                     ARDTGATYFIIKGERELPDIYARLADELRNQYQLAFYSDLSVPDQWHALRITSSAGQQ
                     LRIPLGYFP"
     gene            complement(1774..2874)
                     /locus_tag="VNN25_02235"
     CDS             complement(1774..2874)
                     /locus_tag="VNN25_02235"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXH90371.1"
                     /translation="MAKPRTTAPSIASQGFPKLDLPIRPPYPPMEAKRVDKIPAGDVW
                     QFEPKWDGFRAVVFKSGDDVVVQSKAGQPLGRYFPEVVEAVRGLKQKEFVLDGEIVIS
                     VGGRLSFDHLLQRIHPAESRVRKLAAETPAHYFVFDLLVVKGKSLIEKTVEARREQLE
                     RFFESVPEDSLIRLSPATTDRKVAGDWFNKFTKAGLDGVMAKRLGEPYHSGDREGMVK
                     VKHLKTADCVVGGFRYGEGTRTVGSLLLGLYDSEGRLVHIGHTSSIRKDDRAALTKQL
                     EALRGENPFEVRVPGGPSRWATEKTGEWEPVKPQLVCEVEYDYFSQGRFRHGSKFLRW
                     RPEKKPRQCLIEQVTGKNDVGRMNDEWALIGS"
     gene            complement(2907..3872)
                     /gene="ligD"
                     /locus_tag="VNN25_02240"
     CDS             complement(2907..3872)
                     /gene="ligD"
                     /locus_tag="VNN25_02240"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXH90372.1"
                     /translation="MAASKRLLIPTDVTDVVLNVGSREVRLTNLPKLFWKNLKITKRD
                     LLQYYADISPYLLPHLAGRAMVMKRYPNGAEGDFFYMKRAPEPRPDWIDICPIPHGAG
                     PVQFPIVQDLPSLLWLINLGCIDLNQWYARCDDYNRPDVLHFDLDPGPGADFKMIREV
                     ALRVHEILDHLGMQTFAKTTGSKGIHVYIPIVRGPLQKEVWTFAKAIAIELEARNPNI
                     ITAEYRKVKRPYARVLVDYNQNAWGRTLASIYSVRPTPFAGVSTPVTWEEISEGIDIQ
                     DFRIDNVRERVAEVGDLWKAVAVGKKRFDLTTVFSAPKPKTTKKR"
     gene            complement(3904..4521)
                     /locus_tag="VNN25_02245"
     CDS             complement(3904..4521)
                     /locus_tag="VNN25_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90373.1"
                     /translation="MPSRIFLLSPANTSGIRAKQFISPRASFEAALQYRSPEGVEIAK
                     AFAFMSSLYFRGKIGYAQHFAKPAAVDGCGVYVITPGFGLVPPDWRLTSERMKVMSRT
                     PVDGSEQNYRKPLERDALSLAATLEHDAEVVLLGSVASGKYVDILWPIFETRLRFPAA
                     FAGLGDMSRGGLMLRAVRANRELEYATLDAPRHRTDGNGKIPEGW"
     gene            4664..5332
                     /gene="modB"
                     /locus_tag="VNN25_02250"
     CDS             4664..5332
                     /gene="modB"
                     /locus_tag="VNN25_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HXH90374.1"
                     /translation="MTLHHILLFTLAVAAISTVCIAPVALALAAWSWRAGAKGLVLDA
                     LAALPLVVPPTAVGFVLLEILSRRSVVGALLERIGVEVLFTPKAVVLACAVMSFPLMF
                     TAFRVAIETTDRRYFDVARTLGASPVRAFARVTLPLAWRGLLSGVLLAFCRALGEFGA
                     TILIAGNIPGRTQTMALAIYDRVQNGNDHEGAMLVALVVGIAWVLVSISSILIMKQRR
                     RLDA"
     gene            5329..6366
                     /locus_tag="VNN25_02255"
     CDS             5329..6366
                     /locus_tag="VNN25_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH90375.1"
                     /translation="MIEIDIRLPLAHFMLEVRCALSANVTAIVGPSGAGKTSLIESIA
                     GLRRAHGRIAIDDAALVDTGRGIDLPPERRSIGYVPQDAALFPHLNVSDNIRFGGADR
                     DRFDSLVAALELAPILSRTPASLSGGERQRVALARALMTRPRLLLLDEPLASIDQPLR
                     ERILLYLRRVRDLGIPMIYVTHQPFEALALSSWCVVLRNGVIAATGPSRSVLADPDVA
                     GSVDNVFEVSEPSHDRELGITRATTSDGLTLVLPYDQVAEARFPIVVRIRADDIVVFG
                     EKPRSISSRNIFEGTVASLRASEGIADLIVTTPAPLTVRITQAAAQDLSLRLGSRVWL
                     ALRSRAFRIVG"
     gene            6391..6678
                     /locus_tag="VNN25_02260"
     CDS             6391..6678
                     /locus_tag="VNN25_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90376.1"
                     /translation="MLIKGMTEQEIRVLQEYRRVGTESLTIAAITAIKHPAGGGESPA
                     LSLVEKGFLTTDAARENFALTPKAKEFLSYDPKPEFEEGGASEEVPAEVAE"
     gene            6824..7198
                     /locus_tag="VNN25_02265"
     CDS             6824..7198
                     /locus_tag="VNN25_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90377.1"
                     /translation="MKDGDKAKSKAAKAKASGKETSSKTAAKAKGGKTVKAVSKKESS
                     SPGKASERKAGSIKASPKIVVKASSKGSGKAIVKAAAGSKAGEKAGRSDDADIRFSNP
                     AIESAFRRAVKKYPNAFRRLTD"
     gene            7080..7631
                     /locus_tag="VNN25_02270"
     CDS             7080..7631
                     /locus_tag="VNN25_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXH90378.1"
                     /translation="MKKPAAPTTPTSDSQTQPSKAPSGGPSRSIPTLSAALPTDSSVR
                     YLTVQDIVELHRAISVEFGGNQAHPGVVDSAFGLLNSVQRPQVMMLGREAYATFPDKV
                     AAFLFALLMNSPFRGGNRRVALASLFAFCELNNRDVDTRLLDEKAAENLFKRVAGHRE
                     LGIPPENVFREIRETMTRVIMPL"
     gene            7675..8175
                     /locus_tag="VNN25_02275"
     CDS             7675..8175
                     /locus_tag="VNN25_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXH90379.1"
                     /translation="MATRKNTKASKKRASRNPDAIALLKKDHEKVRGLFAQLEKSAMR
                     GGPKVQQLVTKIDTELKIHTTIEEEIFYPAFRDAVRSKDDQKMYYEAQEEHHVVKLVI
                     PEVNDSEMSGPEFAAKAKVLQELVEHHADEEEQEMFPSARKAFSRSELQDLGDRLAAR
                     KKELGG"
     gene            complement(8216..8662)
                     /locus_tag="VNN25_02280"
     CDS             complement(8216..8662)
                     /locus_tag="VNN25_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXH90380.1"
                     /translation="MVDAVTNTPATCPECGAKRPDPSRVCPACGARPMRADDKRAADN
                     EVRTEDLEPYVALRYIARLFKVLAILLLVMLIGEVVTGVITSGVGAFMTLLGEATRLL
                     VVAGLLWAGGDITILLIDAGHDLRVARILLGRINATLHEREAREKK"
     gene            complement(8714..>9868)
                     /locus_tag="VNN25_02285"
     CDS             complement(8714..>9868)
                     /locus_tag="VNN25_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90381.1"
                     /translation="RVFGGLNARQKHIFNWITVVIVMLYVTGWIGAIHDVVEQRWEIA
                     RMAKEAEAKVPGVKTPPMKAPVPAPPPPLPAPPIATQIFQPQAPTIAYIDDAMLGFLN
                     PLRGKSGKVFFTTLTPGASIVSGAPGGAKSVLTGGTEKVSSDFTAPEQPGVYKLAIQL
                     NQAMRQLDDFSVITLVPFSEKRAGHIGTYYLGSWPFENGGTPKTPAYADPAGFIEVTQ
                     ANADTPISAHFKLRDFLTKDQPNVWPKYLLLNPKLLDKLELTIQELEATGHPVKHFHI
                     MSGFRTPEYNHGGGNTEGRANLSRHMYGDASDTFVDNDGNGVMDDLNHDGRVDDRDAE
                     VIQQAVERVEIKNPSLVGGVGVYSACCGHGPFTHIDVRGYRARWHGTGSG"
BASE COUNT         1846 a         3022 c         3094 g         1906 t
ORIGIN      
        1 aacgcttcgg cgaatcgcgc aacatccgga aggcgtcatt cggcaagctg gcgtccgcac
       61 ggcttcggcg gccattcgat ctcgtcgtct gctccgacgc gctgcactac gtccccgacg
      121 atgagattca cgccggcctt cccgagctcg tgcgcctcgc cggcggcgtg ctctttctcg
      181 aagtgctcac cgccgacgac gacatcatcg gcgatctcaa cggcatgatc cgccgcccag
      241 cgatctggta ccgcaagctc ttcgagaaag cgggtttgct ccaggtcgcg ccgtatacct
      301 ggttagcgcc tgcgcttcgt tccgaggccg cggagcttga aaagtcttga accacagaga
      361 cacaaaggtc acagaggttt tctctgtgct ctctgtgtct ctgtggtgat ttgcattagg
      421 gaaaatatcc gagcggaatc cgcaactgct gccctgcgct cgacgtgatt cgcagcgcgt
      481 gccactgatc gggaacggag aggtcggagt agaacgcgag ctgatattga ttgcgcagct
      541 cgtcggcgag tctggcgtag atgtccggca gctcgcgctc gcctttgatg atgaagtacg
      601 tagcgccggt gtcgcgggcg atcgcggcca gctttcgcgc ctcgaggtat cccactccga
      661 agcgcatcag gcgtgtgagg atcttgttct tgatgatcgg atagaccggc acgccggcgt
      721 aacgcgcgta gtgaacgaga tgatcgagct tgaagtggct tccctggtcg acgccgtcgg
      781 tgaggatgac cagcgcatga cgtcccggct gtttctgcaa ctcgtacagc atctcgatgg
      841 ccgtgtcgta gaggtgcgtt tcgcctgacg gcttcacgcg gtcgacagaa gcggcagcag
      901 cgttgaagtc gctcgtgaat ggcgtcagcc agaacgtcgt ctggttgaag ccgacgacgg
      961 cgattcgatc ctgcggccgc aggagcttct gcgcgaactg ccgcgcggcc agtttcacca
     1021 ccggcaattc ttcgagcatc gattcggagt agtcgatgag cagaatgacg tagagcggct
     1081 ggtcggatgc aggcgttagc gcttcgattt tccgcggtac gccgttgtcg agcaacgtta
     1141 gctcgccggc ctttggcgca cgattacctc cgacgaccga gaccggaacc tgctgcaccg
     1201 cgacgtcgac ctgctcgctc ggatttgtcc cgaagaagag aacgtcgttg gcttcactgc
     1261 cgtcgcgcgc gtgtgcgact acgcgtgcgt agaccgcgtt cggaaacttg gctgggtcga
     1321 acaccgcgtt gtacggcggg gcgctcgcgc tggcgacctt ttcctcaccg atcaggaaat
     1381 cgacggacga gatcgcgagc tgctccggct tgactacgtt tgcttcgagc cttccaggac
     1441 cggcgtgcag ccggatccgg aacggaggcc gcggatcgtt gacgaccatc tcgtcttcgc
     1501 cggcgacgtt gccctgggcg tcgtagccga ccgcgcgaaa acgcagacgc ctgaggtaat
     1561 cgccgatcac gaccgggaag tcgaccgacc cgccgggctt ttcgtcgaac ttcactccgt
     1621 tgatgaacag cgccacgcgc acgaccggct catgcgtctg caccggcacg aagagattgc
     1681 cgtagacaag cgcggtcggt ttcgaggtga tctgcaccgt ctgcgcgagc gccgaggtgc
     1741 tgatcagaag tacgagaagg gacagtcgtc ggatcacgag ccgatcaacg cccattcatc
     1801 gttcatcctt ccgacgtcgt tctttcccgt cacctgctcg atcaggcact gacgcggctt
     1861 cttctccggc cgccagcgca gaaacttcga tccatggcga aagcggccct gcgagaagta
     1921 gtcgtattcg acttcgcaga cgagttgcgg cttcaccggt tcccattcgc ccgtcttctc
     1981 cgttgcccat cgcgacggcc cgccaggaac gcgcacttcg aacggattct cgccgcgcag
     2041 cgcttccagt tgtttggtca gcgcagcacg atcgtccttc cggatcgatg acgtgtggcc
     2101 gatgtgaacg agccgccctt cggagtcgta gagcccgagg aggagtgagc cgacggttcg
     2161 ggtgccttcg ccatagcgga aaccgccgac gacacaatcc gcagtcttga ggtgcttcac
     2221 cttcaccatc ccctcgcggt cgccggagtg atacggctcg ccgaggcgct tggccatgac
     2281 gccatcgagt ccggctttcg tgaacttgtt gaaccagtcg ccggcgacct tgcggtcagt
     2341 cgtcgcgggc gaaagcctga tcagcgagtc ttccggcacg ctctcgaaga atcgctccaa
     2401 ctgctcgcgc ctcgcctcga ccgtcttctc gatgagcgat tttcctttca ccacgagcag
     2461 atcgaaaacg aagtaatgcg ccggcgtttc ggcggcgagc ttgcgaacgc gcgactctgc
     2521 gggatgaatg cgttgcagca gatggtcgaa agagagacgt ccgccgacgg agatgacgat
     2581 ctcgccatcg agcacaaact ccttctgctt caacccgcgc accgcctcca caacctcggg
     2641 gaagtagcgc ccgagcggtt gtcccgcttt cgactgcacg acgacgtcgt caccgctctt
     2701 gaacacgacg gcgcgaaacc catcccactt cggctcgaac tgccagacgt cgccggccgg
     2761 aatcttgtcg acgcgcttcg cctccatcgg cgggtaaggc ggcctgatcg ggaggtcaag
     2821 cttcgggaag ccttggcttg cgatacttgg cgcggtggtt cgaggttttg ccatttgagt
     2881 ctctgcgtct tctgcgtctc tgtggttcaa cgctttttcg ttgtcttagg tttcggcgca
     2941 gagaacaccg tcgtcaaatc gaaccgcttc ttccccaccg ccaccgcttt ccacagatcg
     3001 ccaacctcgg ccacacgttc gcggacgttg tcgatgcgga agtcctggat gtcgatgccc
     3061 tcgcttatct cctcccacgt caccggcgtc gagacaccag cgaacggcgt tggccgcacc
     3121 gaatagatcg acgccagcgt gcggccccag gcgttttggt tgtagtcgac gaggacgcgc
     3181 gcgtacggcc gtttcacttt tcgatattcg gccgtgatga tgttcggatt tcgtgcttcg
     3241 agctcgatgg cgatcgcctt cgcgaacgtc cacacctctt tctgtaaagg accgcgcacg
     3301 atcgggatgt agacgtggat gcctttcgag ccggtcgtct tggcgaaggt ctgcatgccg
     3361 aggtgatcga ggatctcgtg aacgcgaaga gcaacctcgc ggatcatctt gaaatcggcg
     3421 ccgggaccgg gatcgagatc gaagtgaaga acgtcggggc ggttgtagtc atcgcatcgc
     3481 gcgtaccact gattcaggtc gatgcagccg aggttgatga gccagagaag ggagggcaga
     3541 tcttgcacga tcgggaactg caccgggcca gcgccgtgcg gaatcgggca gatgtcgatc
     3601 caatcgggcc gcggctccgg cgcgcgcttc atgtagaaga aatcgccctc ggcaccattc
     3661 ggatagcgct tcatcaccat ggctcggccg gcgagatgtg gcagaaggta cggcgagatg
     3721 tcggcgtagt actgaagaag atcgcgcttg gtgatcttca ggttcttcca gaagagtttc
     3781 ggcagattcg tcagccgcac ttcgcgcgaa ccgacgttga gcacgacgtc ggtgacatcg
     3841 gtggggatga ggaggcgttt ggatgcggcc acgactgcag attgtgcagc ggttatgcct
     3901 tcgtcaccag ccctcaggga tttttccgtt gccatccgtg cggtgcctcg gcgcgtcgag
     3961 agtcgcatat tcgagctcac ggttcgcgcg aacggcgcgc agcatgagcc caccgcgcga
     4021 catgtcgccg aggcctgcga aggcggccgg gaagcgaagg cgcgtttcga aaatgggcca
     4081 gaggatgtcg acgtacttgc cacttgcgac gctgccgagg aggacaacct cggcgtcgtg
     4141 ttcgagagtg gcggcgagcg aaagcgcatc gcgctcgagc ggctttcggt agttttgttc
     4201 gctgccatcg acgggcgttc gcgacataac cttcattcgt tcggatgtca gacgccagtc
     4261 aggtggaacg agcccgaaac ctggcgtgat gacgtaaacg ccgcagccat cgactgcagc
     4321 gggcttcgca aagtgctggg cgtaaccgat cttgccgcga aagtagaggg agctcatgaa
     4381 cgcgaacgcc ttcgcgatct cgacaccttc gggagaccgg tactggagcg cggcttcgaa
     4441 gctcgcgcgc ggcgaaatga attgcttcgc gcgaatcccg ctggtgttgg cgggcgagag
     4501 taggaagatc cgggagggca cggggtggtg aaacaacgag ggcgccggat gtcatttcgg
     4561 gcttcggcgt cgaggctcga tttttatcac tcgggcgggg acgcccgagt gtcgcgggcg
     4621 agacgcctgg cgagacgccc gcactcctct ggcgtaagct tggatgacat tgcaccacat
     4681 ccttctcttc acgctcgctg tggcggcgat cagcacggtt tgcattgcgc cggtggctct
     4741 ggcgctggct gcgtggtcgt ggcgagcggg ggcgaagggg ctcgttcttg atgcgctcgc
     4801 ggcgttgcca ctggtcgtgc cgccgacggc ggtggggttt gtacttctgg agattctttc
     4861 gcggcgaagc gttgtcggcg ctcttctcga gcgcatcggt gtcgaggttc tcttcactcc
     4921 gaaagcggtc gtcctcgcct gcgccgtgat gtcgtttccc ctgatgttca cggcgttccg
     4981 cgtggcgatc gagacgaccg accggcgcta tttcgatgtg gcccgaacgc tgggcgcgtc
     5041 gccggtccgc gcattcgcgc gcgtgacgct cccgctggcc tggcgcggcc tcctgagcgg
     5101 cgtcctgctc gcattctgcc gcgccctcgg cgagttcggc gcgacgattc tcatcgccgg
     5161 caacatcccg ggccgcaccc agacgatggc tctggcgatc tacgaccgcg tgcagaacgg
     5221 aaacgatcac gagggagcaa tgctggtcgc gttggtcgtc ggcatcgcct gggttctggt
     5281 gtcgatcagc agcatcctga tcatgaagca gcggagacgg ttggacgcat gatcgagatc
     5341 gacattcgtc tgccgctcgc gcatttcatg ctggaagtcc gctgcgcact gagcgcaaac
     5401 gtcaccgcga tcgtcggacc gtccggcgcg gggaagacgt cgctgatcga atcgattgcc
     5461 ggactccgcc gcgctcacgg caggatcgcc atcgacgacg ctgcgctggt cgatactgga
     5521 cgcggcatcg atcttccgcc ggagcgccgg tcgatcggct acgtgccgca ggacgccgcg
     5581 ctctttcctc acttgaatgt cagcgacaac atccgcttcg gcggcgcgga ccgcgatcga
     5641 ttcgactcgc tcgtcgcggc actcgagctt gcgccaatcc tgagccggac gccggcttcg
     5701 ctttcgggcg gtgagcgcca gcgcgtggcg cttgcacggg cgctgatgac gcggccgcgc
     5761 ctgttgttgc tcgacgaacc gttggcgtca attgatcagc cgcttcgcga gcgcatcctc
     5821 ctgtacctga gacgagttcg cgacctcggc atcccgatga tctacgtcac ccaccagccg
     5881 ttcgaagcgc tcgcgctcag cagttggtgc gtcgtccttc gcaacggagt gatcgccgcc
     5941 accggaccgt cccggagcgt gctcgctgat cccgatgtgg cgggaagcgt cgacaacgtg
     6001 ttcgaagtgt cggaaccgtc gcacgatcgc gagctgggga tcacgcgtgc gacgaccagt
     6061 gacggtttga cgctggttct tccctacgat caggtggccg aggcgcggtt tcccatcgtc
     6121 gtgcgaatcc gggcagatga catcgtcgtt ttcggcgaaa agccaagatc gatttcctcg
     6181 cgcaacatct tcgaaggaac cgtggcgtcg ctccgcgcga gcgaaggcat cgccgacctc
     6241 atcgtgacga cacccgcgcc gctgactgtg cggatcacgc aggccgccgc gcaggatctt
     6301 tcgttgcgtc tgggctcgcg cgtctggctc gcgctgcgca gccgcgcctt ccgtatcgtc
     6361 ggctagccgg tccccgttac aatgtgcagc atgttgatca aaggaatgac ggagcaggag
     6421 atccgagtcc tgcaggagta tcggcgggta ggcaccgagt cgctgaccat cgcggcgatc
     6481 acggcaatca agcatccggc cggaggtggc gagagtccgg cgttgtcgct tgtggaaaag
     6541 ggctttctga cgaccgacgc ggcccgggaa aacttcgccc tgacgccgaa ggcaaaggag
     6601 tttctttcct acgatccgaa gccggagttc gaggagggag gcgcctcgga ggaggtacct
     6661 gccgaggttg ccgaataaat tgcacacaaa tcggtgcaat ctataactca cgtaaattca
     6721 gcggtttagg ggtgtggcat tgggtcacaa atgatacctc tgcgcgtaaa cgccattgac
     6781 tcaggaactt agctgacgta gcatgagtcg ccgaggattg gaaatgaaag atggcgacaa
     6841 agcaaaaagc aaagcagcca aagccaaagc ctcagggaaa gaaaccagca gcaaaaccgc
     6901 agcaaaagcc aaaggcggca aaaccgtcaa agccgtcagc aaaaaagaaa gcagcagccc
     6961 cggcaaagcc agcgaaagaa aagccggcag catcaaagcc agccccaaaa tcgtcgtcaa
     7021 agccagtagc aaaggctccg gcaaagccat cgtcaaagcc gcagccggct ccaaagccgg
     7081 tgaaaaagcc ggccgctccg acgacgccga catccgattc tcaaacccag ccatcgaaag
     7141 cgccttcagg cgggccgtca agaagtatcc caacgctttc cgccgcctta ccgactgaca
     7201 gcagcgtccg ttacctgacc gttcaggaca ttgtcgagtt gcaccgcgcg atcagcgtgg
     7261 agttcggcgg caaccaggca catcccggcg tggtcgactc tgcattcgga ctcctcaaca
     7321 gcgtgcagcg gccgcaggtc atgatgctcg gacgcgaggc gtacgcgacc ttccccgaca
     7381 aggtggcagc gttccttttt gccctgctca tgaacagtcc gttccgcgga ggcaaccgcc
     7441 gcgtggctct ggcctccctc ttcgcgttct gtgagctcaa caaccgtgac gtcgacacac
     7501 gccttctgga cgagaaagcg gcggagaatc tcttcaagcg cgtggcagga catcgtgagc
     7561 tcggcatccc gcccgaaaac gtcttccgtg agattcgcga gacgatgacg cgcgtcatca
     7621 tgccgctgtg atgaatgggc acgccttgtg atcttcccgg cgcggaggat tccaatggct
     7681 accagaaaga acacgaaggc atcgaagaaa cgagcgtcgc gcaacccgga tgcgatcgca
     7741 ctcctgaaga aggatcacga aaaggttcgg ggtttgttcg cacagctcga gaaatcggcg
     7801 atgcgcggag gtccgaaggt ccagcaactc gtgacgaaga tcgacacgga gctgaagatc
     7861 cacacgacca tcgaagaaga gattttctat cctgccttcc gcgacgccgt ccgttcgaag
     7921 gacgatcaga agatgtacta cgaggcccag gaagagcacc acgtggtcaa gctcgtcatt
     7981 ccggaggtca acgattcgga gatgagcggc ccggagtttg cggcgaaggc aaaggtgctc
     8041 caggagctcg tcgagcatca cgcggacgaa gaggagcaag agatgtttcc cagcgcccgc
     8101 aaggcgttca gccgttcgga gcttcaagac ctcggtgacc gcctggccgc gcggaagaaa
     8161 gagctcggcg gctaatcgaa gatggattcg gcccagcgcc actgggccga atcattcact
     8221 tcttttcgcg cgcctcccgc tcgtgcagcg tggcgttgat tctgccgagg aggatgcgag
     8281 ccacgcgcag atcgtgtccg gcgtcgatca ggaggatcgt gatgtcgccg ccagcccaaa
     8341 gcaatcctgc caccacgaga agccgcgtcg cctcgccgag gagcgtcatg aacgcgccga
     8401 cgccgctcgt gatcacgccg gtgacgacct ctccgatgag catgacgagg agaaggattg
     8461 ccaggacctt gaaaagccgc gcgatgtagc gcagcgcgac gtacggttcc agatcttcag
     8521 tgcgcacttc attgtcggca gcgcgcttat cgtcggcgcg catcggccgc gcaccgcatg
     8581 ccgggcagac gcgcgacgga tcgggacgct ttgcgccaca ttcgggacaa gttgccgggg
     8641 tgttcgttac tgcgtcgacc acgttacctc ctgatatttg ctccgggtgc cggctctcgt
     8701 gtgccggaaa acatcatcca cttcccgttc cgtgccaccg cgcgcggtac ccgcgcacgt
     8761 cgatgtgcgt gaacgggccg tggccacaac atgcggaata aacgccaact ccgccgacga
     8821 gcgacggatt cttgatctcc accctttcca cggcctgctg tatgacctcg gcatcgcggt
     8881 catcgacgcg accgtcgtgg ttgaggtcgt ccatgacgcc gttgccatcg ttgtcgacaa
     8941 acgtgtccga ggcgtcgccg tacatgtgcc ggctcaggtt ggcccgcccc tcggtgttgc
     9001 cgccgccgtg gttgtattcg ggcgtgcgga aaccgctcat gatgtggaag tgcttgaccg
     9061 gatggccggt cgcctccagc tcctgaatgg tcagctcgag cttgtcgagc agcttgggat
     9121 tcaacagcag gtacttcggc cagacattcg gctgatcctt ggtcagaaag tcgcgcagtt
     9181 tgaaatgcgc ggagatcggc gtgtcggcgt tcgcctgcgt gacttcgatg aacccggccg
     9241 gatccgcata cgctggcgtc ttcggtgttc ctccgttttc gaacggccaa ctgccgagat
     9301 agtaggtgcc gatgtggccg gcccgcttct ctgaaaacgg aacgagcgtg atgacgctga
     9361 aatcgtcgag ctgccgcatg gcctgattga gctggatggc aagcttgtag acgcccggct
     9421 gctccggcgc ggtgaagtcg gacgacacct tctcggttcc gccggtcagc accgacttcg
     9481 cgccacccgg cgcgccggag acgatcgaag cgccgggcgt aagcgtcgtg aagaacacct
     9541 tcccgctttt tccgcgcaac ggattgagaa agccgagcat ggcgtcgtcg atgtaggcga
     9601 tcgtcggcgc ctgcggctga aaaatctgcg tcgcgatcgg cggagccggc aagggtggcg
     9661 gcggagccgg cacaggcgcc ttcattggcg gcgtctttac tcccggcacc ttcgcttccg
     9721 cctccttcgc catgcgggcg atctcccatc gttgttcgac gacgtcgtga atggcgccga
     9781 tccaaccggt gacgtacagc atcacgatca cgaccgtgat ccagttgaag atgtgcttct
     9841 gccgcgcgtt cagcccgcca aacacgcg
//
LOCUS       DATLVW010000067         3877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_209501, whole genome shotgun sequence.
ACCESSION   DATLVW010000067 DATLVW010000000
VERSION     DATLVW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209501"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VNN25_02290"
     CDS             <1..1408
                     /locus_tag="VNN25_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH90382.1"
                     /translation="FSDELAIATDGKAKIFSVSVKDRGAIPLAGHAGKAFWYSTKNGC
                     FVTSTFYYDPPDKPLPPWVKSWCDQKLADTYRKGPGDESSPPSDKWNGQWVLSRHPST
                     YLYRGFTNEYPKGSIAALNMTTLVDRYHFGSTFPHTFADPGAGFYGALMITPIGDELT
                     AAFAEQIITSEQLGKHSVTDYLAVSFSSTDYIAHWFSPASLESEDNILRLDVTLAKLL
                     AFIDKEVGLKNTIVVLSGDHGGVEYPEYLKTLRINSDRLKPDEIEGAATKALKARYPS
                     IPEDIWNCPRKPKEPPTPCIFTWSQPSFYLHQQVIAANKLNQPEVEAVMASGLMKLPG
                     VAMALTSADMRSGVGDLDPAFVAQVRRNYNPERSGDVYVVQQPQWQILEAGSNDEGPS
                     TLLQHGSPWTYDTYVPVAFAGMEIPAAKIARRVYTVDVAATLSSLVWTKIPSGCVGVP
                     LTEVVHSEMAGPTATPSH"
     gene            1875..2384
                     /locus_tag="VNN25_02295"
     CDS             1875..2384
                     /locus_tag="VNN25_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90383.1"
                     /translation="MQPSQAQAQSAEAPRISEHILDGARDPGLFLPAELLDVLLQGLH
                     PKATFRMHAREALAKDIREMGYTEDDFWNKLQQLSAPYRALTSRPKNLAVHHVKTPDG
                     KVASFLIDVDQCVARHKLLQSARATFGAEAFQRFLYAAVAPQVWRSDTTQSPDPAQEL
                     IFVAGGCRQ"
     gene            2399..3613
                     /locus_tag="VNN25_02300"
     CDS             2399..3613
                     /locus_tag="VNN25_02300"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90384.1"
                     /translation="MTAALFVLAAPATQAGQLYVHVGFQFSFHNDGIYYADAYYDYDI
                     FDFFTANVWGVATLNGSGQASQYFGPTMANHPDVASGQWDFSGAFMSCYEAHATAFED
                     NGTTQGAGSGVKCAYWQPDSYNVLISTNVDGVLHTLRNDRYQEGTSVSYHPDTPPGFE
                     FIGWSGVVNSSEESITFRMPSNDTSLCGNFTYIGTGGDPPNTDGGTENEDLPCFPGID
                     VNCYGSPVIINFEKGDYRLTGSSAPVLFDMTGNGHPRAMGWTAAGADEAFLWLDRNHN
                     GRVTSGAELFGNFTPLQNGQLAKNGFEALRELDTNGDGVVDEQDPIWSRLMLWKDLNH
                     NGISEPNEILPIAGSGVISIDLHDHWSGRRDASGNLFKYESLVSITNGPGQAIRKHPV
                     YDIFFAYVPYVP"
BASE COUNT          788 a         1207 c         1142 g          740 t
ORIGIN      
        1 gttcagcgac gaactggcga ttgcgaccga cggtaaggcg aagattttct cggtctcggt
       61 gaaggatcgc ggcgcgatcc cgctggcggg ccacgccggc aaggcgttct ggtactcgac
      121 caaaaacggc tgcttcgtca ccagcacctt ctattacgac cctcctgata aaccgctccc
      181 gccgtgggtg aagtcgtggt gcgatcagaa gcttgccgac acgtacagga agggccccgg
      241 tgacgagagc agtcctccct ccgacaaatg gaacggccaa tgggttctgt cccggcaccc
      301 ttctacgtat ctctacaggg gcttcaccaa cgagtatccc aaaggcagca tcgccgcgct
      361 gaacatgacc acgctcgtcg acaggtacca tttcggcagc acgtttcccc acacgttcgc
      421 ggacccagga gcggggttct acggcgccct gatgatcacc ccgatcggag acgagctaac
      481 cgccgccttt gcggagcaga tcatcaccag cgaacagctc ggaaaacaca gcgtcacgga
      541 ttacctcgcc gtcagcttct cgtcaacgga ctacatcgcg cattggttca gccccgccag
      601 cctcgaaagc gaggacaaca tcctccggct cgacgtcacg ctggcgaagc tgctggcgtt
      661 catcgacaag gaggtcgggc tgaagaacac gatcgtcgtg ctctccggcg atcacggagg
      721 ggtggagtac cccgaatacc tcaaaacgct caggatcaat tccgaccggc tcaaaccgga
      781 cgaaatcgag ggcgccgcca cgaaggctct gaaagcgcgg tacccatcga tccccgaaga
      841 catctggaac tgcccgcgaa aaccaaagga accgcccacg ccgtgcatct tcacgtggtc
      901 gcaaccctcc ttctatcttc accagcaggt gattgccgcg aacaaactga atcagcccga
      961 agtcgaggcg gtgatggcga gcggcctcat gaagctgccc ggcgtggcca tggcgctcac
     1021 cagcgccgac atgcgcagcg gcgtcggcga tcttgatccg gcgttcgtgg cgcaggtccg
     1081 gcggaactac aacccggagc gctcgggcga tgtgtacgtc gtgcagcagc cgcagtggca
     1141 gatcctggag gccggctcca acgacgaagg ccccagcact ctgctccagc atggatcgcc
     1201 gtggacgtat gacacgtatg taccggtcgc gttcgcgggg atggagattc ccgcggcgaa
     1261 gatcgcgcgc cgcgtctaca cggtcgatgt ggcggcgacg ctgtcgtcgc tggtctggac
     1321 gaagatcccg tcagggtgtg tcggggtgcc gctgaccgag gtggtgcatt cggaaatggc
     1381 cgggcctact gccacaccga gtcactgaag ccggccacgc gcgtaactcc atcggcgcca
     1441 tcgatacgcg cgagcacttc gcgcagcacg gccaggtcga gaatcgccat tcttcgcaag
     1501 ctaacgggaa agcccagcga ggcaacctcc atgcgctttc ttgcacacgt cacgttctcg
     1561 catgatttga ttcattgact tcttacggaa gtatcactac gttgctctga atcaacgaaa
     1621 tcaagagagt gaggttactt atgcgacgct ggccaatgtt ggtctgtctc atgctcccgg
     1681 gcttcgcggt ccacgcggca ccgcccgtca ccacaacgcc tggcgttagc gccgtcatcg
     1741 gccggggatc atcggggaag cctgcgtggg agtggagtga cgaggaacgt atcagagtgc
     1801 ggtttgaccc cgcctcaatt cgtgagcggt ccgcagcaca cgcagccgcg ctgccgccat
     1861 acgcccaacc acacgtgcag ccctcccaag cacaggcgca gtccgccgaa gcaccgcgga
     1921 tctcggagca catccttgat ggagcacgtg accccggcct cttcctccca gccgagttgc
     1981 tcgacgtgct cttgcagggg ctgcatccca aggcaacatt ccgaatgcac gcacgcgagg
     2041 cactggcaaa ggacatccgc gagatgggct acaccgagga tgacttttgg aacaagcttc
     2101 agcagctctc ggctccttac cgcgcgctaa cgtcgcgacc gaagaacctc gctgtccatc
     2161 atgtgaaaac gcctgatggc aaggtggcgt cgttcctcat tgacgtagac caatgcgtag
     2221 cccgtcacaa acttctccag tccgcccgcg cgacgttcgg cgcagaggcg tttcagcgtt
     2281 ttctgtacgc tgctgtcgct cctcaggtgt ggcggtccga taccacacag tcgcccgatc
     2341 cggcgcagga acttatcttc gttgcagggg ggtgccgtca gtgaaacgtt ggattctagt
     2401 gaccgccgct ctctttgtgc ttgccgcgcc ggccacgcaa gcgggtcagc tctacgtgca
     2461 cgtcgggttc cagttttcat ttcacaacga tgggatctac tacgccgacg cttattacga
     2521 ttatgacatc ttcgatttct ttacagcgaa tgtctggggg gttgccacac taaacggcag
     2581 cggacaagcg tcgcagtatt tcggccccac aatggccaac catcctgatg tagcttcagg
     2641 gcaatgggat ttttctggcg cgttcatgtc ctgctatgag gcgcacgcga cggccttcga
     2701 ggacaatggc acgacgcagg gcgcgggcag cggagtgaaa tgcgcgtatt ggcagccaga
     2761 ttcttacaat gtattaatct ctaccaacgt tgacggagtc ctccacacct tgcgcaatga
     2821 tcgctaccag gaagggacct cggtttccta tcacccagac acaccgccgg gtttcgagtt
     2881 catcggttgg tcgggtgtcg tgaactcatc ggaggagagc atcaccttcc ggatgcctag
     2941 caatgatacg agcctatgcg ggaacttcac ctacattggc accggtggcg atccgcctaa
     3001 caccgatggc gggaccgaga acgaggacct tccttgtttc ccgggtatag acgtgaattg
     3061 ctacggctca ccggtcatca tcaatttcga gaagggcgac taccgcttga cgggcagcag
     3121 tgcgcccgta ctcttcgata tgaccgggaa cggccatccg cgggcgatgg gctggaccgc
     3181 cgccggcgca gacgaggcat ttctctggct cgatcgcaac cacaatggca gggtgaccag
     3241 tggcgcggag ttgttcggca acttcacacc gctgcagaac ggtcagcttg ccaagaatgg
     3301 ctttgaagcg cttcgcgagc tcgatacgaa cggcgacggc gtcgtcgacg aacaagaccc
     3361 gatttggtcg cggctcatgc tgtggaagga cctgaaccac aacggtatct cggaaccgaa
     3421 cgagatcctg ccgatcgccg gaagtggtgt catctcgatt gatctccatg atcactggtc
     3481 gggaagacgc gatgcgtctg gcaacctgtt caaatatgag tcgctcgtct cgatcacgaa
     3541 cggccctggg caggccattc ggaaacaccc tgtttacgat atcttcttcg cttacgttcc
     3601 atacgttcca tagcgcatct gctgcccggc gggcgctcgg gtccgccggg atttgctcgc
     3661 tctgaggatg acctgtcgcg cgagccgtca gggtgtgtcg gcgtgccgct gaccgaggtg
     3721 gtgcatgcgg aagtggctgg gcctactgcc acaccgagtc actgaagccg gccacgcgcg
     3781 taactccgcc ggcgccgtcg atacgtgcga gcacttcgcg cagcacggcc aggtcgagaa
     3841 tcgccattcc gaacggcgaa acgatcgtcg ggacgcc
//
LOCUS       DATLVW010000068         9163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_335144, whole genome shotgun sequence.
ACCESSION   DATLVW010000068 DATLVW010000000
VERSION     DATLVW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335144"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..701
                     /locus_tag="VNN25_02305"
     CDS             126..701
                     /locus_tag="VNN25_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90385.1"
                     /translation="MSSSTPNPDTPAAGTPAVGPQTRDAQERIARLRAIADDFPDDAS
                     PKPLTTAERRLASRTSVAFLEKAAVFAEAAPNVGVALSVDTLVLRDAIASDLATGGIV
                     DETRALTSRLEDANARKKLKAVRLARGIYRVAKSYVTTDLGNVVKPHVDEMKRTLQRP
                     YTSKKKTAPAKPPATTPAATPKPPDSTSQQK"
     gene            882..1178
                     /locus_tag="VNN25_02310"
     CDS             882..1178
                     /locus_tag="VNN25_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXH90386.1"
                     /translation="MKKTTGKSQTKRSANIFTDLGFQPHEAAVMLLRCELAEALRKWI
                     DREGITQAQAAERLGVAQPGISEIARNKVHKLSLDYLVGLCAKAGVAVKVRTAA"
     gene            1457..2203
                     /locus_tag="VNN25_02315"
     CDS             1457..2203
                     /locus_tag="VNN25_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90387.1"
                     /translation="MKRSWSGEIQKLAELESFDAAAFITDAKDKSQQDCGDFVLALAL
                     VFNDIKGLLAAISLLVEEVPGDRTTPSRELGAFAGVYFQVIRTLFGALRELLDLVKRE
                     EATHKGPSFQTVVRQMPSNQRKAWAKLVAASEANKSSDPDVNFLIRARNAVAYHYETA
                     AIGQGFCRAFKDASDEPFLSRGSTIASTRFYFADRAAQSFLQLQLGDEDIESYFSRDP
                     KLIHDIAFALFSVVTGFITSRGCAWRKPAN"
     gene            complement(2366..4066)
                     /locus_tag="VNN25_02320"
     CDS             complement(2366..4066)
                     /locus_tag="VNN25_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90388.1"
                     /translation="MDTSFAIRLMYSNVAGIPRDPEKLWSLAKTSAPGDDLISLLAVI
                     SNALIFLGATNQKVHAELEAEFAQADLREALTRHRAAAEGRVILFNRPAHLITLKLLL
                     GIEHAAGTDASFPRIGEITLHANDYVESPEEIWDADPDLLGILAEFAPVWELLNPRDV
                     FQLFVRTYLLLTEHVTAHEGMVKLFVAQLGAAPPDLLVDGLRIDDYLALVFGIFTNLR
                     TAVTKDKTCIIDMHQFFRVTTLPHEGLTAFLNRRSGDVSAFYDELKLNVSSTDEFRAY
                     IIEGTKAIDVTVIKQRPIFCRSDGRHSVLDGRFLIELLSTTLYWTLFDAIDEKKARNT
                     FSTFWGECFEAFVLRELAFFYPPASAILHTRVPIEDGEIDAMLDFGDFIIVFEIKSGL
                     LAKDPRLMRDPKKLHDELHKKFVDGTGVWQLVKAVRAVANGEVGTSRKGCRIYPVLVG
                     DEPVLQCLVANRYLDQQFGAQLTDRPQNVAPLTVMLIDEMEEGLPYFASGSIAWQEAL
                     DARFEPDGVSPDSFQTALAAIGAAKKIPKQRNDFLSEQGERIGKLIFDRYHFGDTKTE
                     "
     gene            complement(4257..4682)
                     /locus_tag="VNN25_02325"
     CDS             complement(4257..4682)
                     /locus_tag="VNN25_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH90389.1"
                     /translation="MEPIDISGLPLDALLVLDSAPIIYLLEDHPRLRRRFEPLFASHA
                     AGHVRFAVTTITIAEVLTGPLGAGEEVLARRYRSILESWQVVDMDAGIAESAARMRGL
                     FRLKLAEAVQVASALAINADALVTHDRDFSSVTGLRVIS"
     gene            complement(4670..4924)
                     /locus_tag="VNN25_02330"
     CDS             complement(4670..4924)
                     /locus_tag="VNN25_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXH90390.1"
                     /translation="MSRITRKGAEEARNQMPALLEAAEKGTSTIITRHGHPVAALVPL
                     EQFSARGQQKALLPLKGTGRGLWGKSSARAIRALRDEWNR"
     gene            complement(4977..7403)
                     /gene="priA"
                     /locus_tag="VNN25_02335"
     CDS             complement(4977..7403)
                     /gene="priA"
                     /locus_tag="VNN25_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXH90391.1"
                     /translation="MPAGLSEFAEVALPVAVHGTFTYAIPPSLRDSVRLGSRVEVPFG
                     AKRNTGFVVALTDRAPESGKIKPILGVLDDDEPALLPEIIDLCRWAADYYIAPLGEML
                     RTALPANMSARGKREISLAGDDAMIAAALEAKQILDGDLALIEELRRRSLPFAAALEA
                     GSRSTIDRLRDAGIVTVADRVTDAKGVRYDRFVILETPPDGLTAKQQQAVEVLASRGG
                     EMSVRALENAGVSAAVLGALSKKSVIRIERRARRHTLDAFLAGLDPASVGEIRHSEEQ
                     RAAIAAIRAGMGTFAPFLLEGVTGSGKTEVYIELMRDAVRRGEQALLLVPEISLTPVF
                     ASRLKERFGERIAILHSSLSASERFDQWWRARRGEVDVAIGPRSALFTPFQRLGLIVV
                     DEEGDSAYKQDESPRYNARDLAVVRAQFRGIPIVLGSATPSLESRENAARGKYTLIRM
                     LSRVESRPLPIADVIDLRKERAEKEDKGFVIFSNPLKERLKETFAAGEQAIILMNRRG
                     YAPYLLCRDCGHDFRCRDCSVTLTVHRREGLLICHYCGLRKKIPEHCPLCNGVTMQPI
                     GFGTEKVEERFRRDFPGINVAVLDRDSTRKKGELVRILDAFRRQETQALIGTQMLSKG
                     HHFPSVTLTAVLNADSILGYPDFRSAEKTFYLLTQVAGRSGRGDLIGTVLIQSAFPTH
                     YAIQHALRHDYEAFYESEIQFRKTFHYPPVTSMIAILFRGELLGDVDRAALECGRKLE
                     EALKPIANTRIQGPAPAPMARIKGVYRYQILLRSPHRTAIRKAVEAVMIGKKWKGVEV
                     AIDVDPINIL"
     gene            complement(7409..8260)
                     /locus_tag="VNN25_02340"
     CDS             complement(7409..8260)
                     /locus_tag="VNN25_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90392.1"
                     /translation="MRRLIIPILLLAAAHPLAAAFADHDLVVPVVGRAAGGAGRLFLT
                     ALWITNADDHPATLTLSYLESGHANPSPRQTTVLLAPRETHVFDPLGPPILTSINSIG
                     ALRIEANADIVASARIYGYLPEAGPSTAVSMALTAIPTRLGVGNGQSALLHGDGALDA
                     RYKLYLVETAGAALSVVVSIEDLHGQTRAEKRIFLDRFEHVSADAHDLFPSVKLDQAV
                     VRIRGVNGEGRVIAAGAQIVAGSQDACAYEMSFAPEPHTRIRAPEAAVYVAIALVVGL
                     AIIWRRK"
     gene            8328..>9163
                     /locus_tag="VNN25_02345"
     CDS             8328..>9163
                     /locus_tag="VNN25_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90393.1"
                     /translation="MAALLYLLSAAGALLLVRRFLTLSRHVSAVLILLPLCLTGRALV
                     TGNVYAPIDLAYHFDPLASMSTKVGIEQIANPLPSDVTVQFLPWNAALRWAIAHRQWP
                     LWNPFELGGNVLAAAAQSAPFHPVTLLGLLLPMPDALGYMASMAYFLAALGMFLFLRN
                     LELRSLPSLFGAAGWMLSTYVVTYTHTAHGNAIALLPFVMLGARMVARSPGVRAASLL
                     AGALILLTLCGHPETSLHVIALATVYLIFCAREKWGLVIISGLGAGIAALLLTAFFLL
                     PM"
BASE COUNT         1765 a         2753 c         2842 g         1803 t
ORIGIN      
        1 tgacaccacg cggatggcgt gtcgccttgc cgcgtctgca ccctcgtcgt ccggcgccaa
       61 tggtggcgct gacgactcca tgaaccccat tcgaagggtg cgacaacagc atcccgaggt
      121 atccgatgtc ttcttccaca ccgaatcccg ataccccggc ggcaggaacg cctgccgtcg
      181 gtccccaaac ccgcgacgcg caggagagaa tcgctcgcct gcgcgccatc gctgacgatt
      241 ttcccgacga cgcatcaccg aaaccattga ccacggcgga gcgccggctg gcgagccgca
      301 cgtccgtcgc attcctggag aaggctgctg tcttcgccga ggctgctccg aacgtcggag
      361 tggcgctttc cgtcgacacg ctggtattac gcgacgccat cgcctcggat ctggccaccg
      421 gaggcatcgt cgacgagaca cgagctctaa cctcgcgcct ggaggacgcg aatgcccgca
      481 agaagctcaa agcggtgagg cttgcgcgtg gcatctatcg tgtggccaag agctacgtga
      541 ccaccgacct ggggaatgtc gtgaaacccc atgtcgacga gatgaagcgc acattgcagc
      601 ggccgtacac gagcaagaag aagacggcgc cagcaaagcc gccggcaacc acgccagcgg
      661 caacgcccaa gccgcctgat tcaacgtcgc aacagaagta accgaggggc cggaggaggg
      721 tatggtttgg caatccacct ccccggctcc tcacacgacc cgtagttacg agtcgagcac
      781 tacccgaccc gccacaaacc gcgggttgga ttttatgatg aaccgacatc cctcgacatc
      841 gaagtggcga aacggcggta ccgtttgatc ggagtcaatt catgaagaaa acgacgggta
      901 agtcccaaac gaaaaggagc gccaacatct tcaccgatct cggctttcag cctcatgaag
      961 cggccgtcat gttgctgcga tgcgaactgg cggaagctct gcgcaaatgg atcgatcgag
     1021 aagggatcac ccaggcgcag gccgccgagc gtctgggcgt ggcgcaaccg ggcatctcgg
     1081 aaattgcccg aaacaaagtg cacaagctat cgctcgatta cctcgtcgga ctctgtgcca
     1141 aggccggggt tgcggtgaag gtgaggacgg cagcgtaggg ccgtattcat ccgctgtttg
     1201 gcaggatgct catcgaactt tacctgatac gcgcaagctg accttcagtt tgctggctct
     1261 tccgtcagct ctcgtttatc tatcttcggc tcccctgcga cgtagccaga tgccgcccat
     1321 tctctggcca cggagtcggg gaaggggtca ggtctaaagt gtagagtgcc gagaacgatg
     1381 cgtcgctaag tcgagcaggc tgaatcggca ctctagactt tagaccgcaa tcggcacccg
     1441 actagaatga atcagcatga agcgaagctg gtccggtgaa atccagaagc tcgcagagct
     1501 cgagagcttc gacgcggcgg cgttcatcac agacgcaaaa gacaaatcgc agcaagattg
     1561 cggagacttt gtacttgctc tcgccctcgt gttcaacgac atcaagggcc ttctcgcggc
     1621 aatttccctt ttggtcgaag aagttcccgg agatagaaca actccgagtc gagagctagg
     1681 agctttcgct ggagtctatt tccaagtcat ccgcaccctt ttcggcgcgc ttcgcgagct
     1741 cctcgacctg gtgaagaggg aggaggcaac tcacaaaggc ccgtcgttcc aaaccgttgt
     1801 cagacagatg ccaagcaatc agcgcaaagc ttgggcgaaa ctcgtagcag catcggaagc
     1861 aaacaaaagc tccgatcccg acgtcaactt cctgatccgt gcacgcaacg cagtcgcgta
     1921 tcactacgag acggccgcta tcgggcaagg cttttgccga gcattcaagg atgcttcaga
     1981 tgagccgttc ctgtctcgag gttcgactat tgcgtcgaca cgcttctact ttgcggatcg
     2041 ggccgcccag tcttttcttc agctccaact cggcgatgag gacatcgaaa gctacttttc
     2101 aagagacccg aagctgatcc acgacatcgc cttcgcgttg ttctcggttg tgacggggtt
     2161 catcacctcg cgcggctgtg cttggcggaa gccggccaat tgaaagggaa ctcacgaacc
     2221 ggactgagaa cgccgcttct ccaagcgtcg ggggcgccac ccctttcaag gcggaaggcg
     2281 cgtccagcga gtcaggagcg atggtcggca agaacttcga tgcaaaccga gaaacccgca
     2341 ggggcaccac tcggagttat ggtcgtcact cagtcttcgt gtcaccgaag tgatatcgat
     2401 caaaaatcaa tttacctatt cgttcgccct gctcactcag gaagtcgttt cgttgctttg
     2461 ggatcttctt tgcggcgcca atggccgcaa gcgctgtttg gaaggaatcg ggcgatactc
     2521 cgtcgggctc gaatcgcgcg tcgagcgctt cctgccacgc gatgctccca cttgcgaagt
     2581 agggaagccc ctcttccatc tcgtcgatca gcatgaccgt gagcggggcg acattttgcg
     2641 gtcgatcggt aagctgcgcc ccaaactgtt ggtcgaggta gcgatttgcg acgaggcatt
     2701 gcagcaccgg ctcgtcgccg accaagactg gatagatgcg gcatcccttc cggctcgtgc
     2761 cgacctcacc attcgcgacc gcgcgaacag ccttaacgag ctgccagacg ccagtgccgt
     2821 cgacgaactt cttgtgcagt tcgtcatgta gcttctttgg atcgcgcatc aggcgtgggt
     2881 ccttcgctaa taagcccgac ttgatctcga agacgatgat gaagtccccg aagtcgagca
     2941 tcgcatcgat ctcgccgtcc tcaataggga cacgcgtgtg aaggatcgca gatgcagggg
     3001 ggtaaaagaa tgccaattcc cgcaaaacga aagcctcgaa gcactcgccc caaaaggtcg
     3061 aaaacgtgtt cctcgccttt ttttcatcga tcgcatcgaa gagcgtccag tacagcgttg
     3121 tcgaaagcag ctcgatgagg aagcgtccgt ccaaaacaga atgccggccg tcggagcgac
     3181 agaagattgg tcgttgcttg atgaccgtca catcgatcgc ctttgtacct tcgatgatgt
     3241 aggccctgaa ttcgtccgtg ctggagacat tcagtttcag ttcgtcgtag aaggcggata
     3301 catcaccact acggcgattt agaaatgcgg tgagcccctc gtgcgggaga gtggtgacgc
     3361 ggaagaactg atgcatgtcg atgatgcacg tcttgtcttt cgtcaccgcc gtacgcagat
     3421 tcgtgaagat gccgaacacc agcgcgaggt agtcatcgat tcgcagaccg tcgacgagga
     3481 gatcaggagg cgctgcgccg agttgtgcaa cgaagagctt caccatcccc tcgtgggcgg
     3541 ttacgtgctc ggtcagtaga aggtaggtcc gaacgaagag ctggaagaca tcacgcggat
     3601 tcagaagctc ccacaccggc gcgaactcgg cgaggattcc gagaagatca ggatccgcgt
     3661 cccaaatctc ctcaggcgat tccacgtagt cgtttgcgtg caaagtgatc tctccgatgc
     3721 gagggaagga cgcgtcggtg ccggccgcat gctcgattcc gagcagcagc ttcaatgtga
     3781 tcaggtgagc cggcctgttg aagaggatca cgcgcccctc tgcggccgcc cgatgtctcg
     3841 ttagtgcttc ccgtagatcg gcctgagcaa actccgcctc taactcagca tgcaccttct
     3901 gattcgtggc tccgagaaat atgagagcgt tgctgatgac agcgagcaag ctgatcagat
     3961 catcgccggg agcgcttgtc ttcgccagac tccagagctt ctctggatct cgtgggattc
     4021 ccgcgacgtt ggagtacatc aggcggattg cgaacgatgt gtccacctga gtagtgtacg
     4081 tcgggccgcc tgatggaaaa catcgtggga ccgaagcgac gggacttggt agcgaaggcg
     4141 ggatagattg tggcttgagg gtcaggtcga aaccgaaact gacggcatgt cgcgcggatc
     4201 gaatggttca ggcgaagggt cgtctgtttc agtttcgacc tgaccccttc cgttgatcac
     4261 gagataaccc tgagcccggt cacactcgaa aagtctcgat catgagttac gagcgcgtcc
     4321 gcattgatcg cgagtgcgct cgcgacctga accgcttcgg cgagcttcag acgaaacaaa
     4381 ccgcgcatcc gggcggcact ctcggcgatg cctgcgtcca tatcgaccac ctgccacgat
     4441 tcgagaatgc tgcgatacct gcgtgccagc acttcctcgc ccgcgccaag aggacccgtc
     4501 aggacctcgg caatcgtgat cgtcgtaacc gcgaagcgga cgtggcccgc cgcatgggat
     4561 gcgaaaagcg gttcgaagcg gcgcctgagt cggggatgat cctcgaggag atagataatc
     4621 ggcgccgagt cgagaaccag cagcgcatcg agcggtagcc cggaaatgtc tatcggttcc
     4681 attcgtcacg cagggcgcgt atcgcgcgcg cactcgattt cccccataga ccgcgcccgg
     4741 ttcctttcag cgggagaagc gccttctgct gccctctggc agagaactgc tcgagcggaa
     4801 cgagagcggc aacgggatga ccgtgcctcg tgatgatggt ggacgtaccc ttttccgccg
     4861 cttccagcaa cgccggcatc tgatttcgcg cttcttcagc tcccttgcgt gtgatgcggc
     4921 tcacgatcgt catactgtac agggctgtac ggaacgcgtc aaggcggctt ggggggctac
     4981 agaatattga tcggatccac gtcgatcgcc acctccaccc ccttccactt cttgccgatc
     5041 atcacggctt ccaccgcttt gcggatggcg gtgcggtggg gggagcggag gaggatctgg
     5101 tagcggtaga cgcctttgat tcttgccatc ggggcggggg ctgggccctg gattcgcgtg
     5161 ttggcgatcg gtttgagcgc ttcttcgagt ttgcggccgc attccagggc ggcgcgatcg
     5221 acgtctccga gtagttcgcc gcggaagagg atggcgatca ttgatgtcac tggcgggtag
     5281 tggaaggtct tgcggaactg gatctcggat tcgtagaagg cttcgtagtc gtggcggagg
     5341 gcgtgttgga tggcgtaatg ggttgggaag gcgctctgta tgaggacggt tccgatcagg
     5401 tcgccgcggc cggagcggcc ggcgacttgc gtcaacagat agaacgtctt ctcggcggag
     5461 cggaaatcgg ggtagccgag gatggagtcg gcgttgagga cggcggtgag ggtcacgctg
     5521 gggaagtgat ggcctttgct cagcatctgc gttccgatta gagcttgggt ctcttgccgg
     5581 cggaaggcgt cgaggatgcg cacaagctcc cctttcttgc gcgtggaatc gcggtcgagc
     5641 acggccacgt tgatgccggg gaaatcgcgg cggaagcgtt cttccacttt ctcggtgccg
     5701 aagccgattg gttgcatcgt tacgccatta cacagcggac agtgctcggg gatcttctta
     5761 cgcaatccgc agtagtggca gatcagcaat ccctcgcggc ggtgtaccgt cagggtcacg
     5821 ctgcaatcgc ggcaacggaa atcgtggccg caatcgcggc acaacaaata cggcgcgtag
     5881 ccgcggcgat tcatcaggat gatggcctgc tcccctgctg cgaacgtctc cttcaatcgc
     5941 tctttcagtg gattgctgaa gataacgaag cctttatcct ctttttcggc tcgctctttt
     6001 ctgagatcga ttacatcggc gatcggcagc ggacgtgatt cgacgcgtga cagcatccgg
     6061 atcagcgtgt atttgcctcg tgccgcgttt tcacgcgatt cgagcgacgg cgtggcggat
     6121 ccgaggacga tggggatgcc gcggaattgc gcgcggacca cggccagatc gcgggcgttg
     6181 tagcgcggcg actcgtcctg cttgtaggcg ctgtcccctt cttcgtcgac gacgatcagt
     6241 ccgaggcgct gaaagggggt gaagagcgcg gagcgcggcc cgatcgcgac atcgacctcc
     6301 cctctccgtg cccgccacca ttgatcgaag cgttcgctgg ccgaaagact gctgtgcagg
     6361 atggcgatgc gctcgccgaa acgctccttc aatcgcgacg cgaacaccgg cgtgagcgag
     6421 atttcgggga cgagtaacag cgcctgctcc ccgcgccgca cggcgtcgcg catcaactcg
     6481 atgtagacct cggtcttccc gctgccggtc actccttcga ggaggaacgg cgcgaaggtt
     6541 cccattcccg ctcgaatcgc agcgatcgca gcgcgctgct cctctgaatg acggatctcg
     6601 ccgacgctgg ccggatcgag cccagccagg aacgcgtcga gcgtgtggcg ccgcgcacgg
     6661 cgctcgatcc ggatcacgct cttcttcgat agcgctccga gcaccgccgc gctcacgccc
     6721 gcgttctcca gcgcgcgcac tgacatctcc cctcctcggc tcgccaacac ttccaccgcc
     6781 tgctgttgct tcgcggtgag accgtcgggc ggcgtttcga ggataacgaa gcggtcgtag
     6841 cggacgccct tcgcatcggt aacgcggtcg gcgacagtga cgatgccggc atcgcgaagg
     6901 cgatcgatcg tcgaccgcga gcctgcttcg agcgcggccg cgaacggaag cgaacgccgg
     6961 cgcagttcct cgatcaacgc cagatcgcca tcgagaatct gtttcgcttc gagcgcggcg
     7021 gcgatcatcg catcatcgcc ggccagcgag atctcacgct tgccgcgtgc cgacatgttc
     7081 gcgggcagcg cggtgcgaag catctcgccg aggggggcga tgtagtaatc ggccgcccag
     7141 cggcagaggt cgatgatctc cggcagcagc gccggctcgt catcgtccag cacgccgagg
     7201 attggtttga tcttccctga ctcgggagca cggtccgtga gcgcaacgac gaatcctgta
     7261 ttgcgttttg cgccgaacgg gacttcgacg cgtgacccga ggcggactga gtcgcgcaga
     7321 gaaggaggga ttgcgtacgt gaacgtgccg tgcacagcga caggcagcgc cacctcggcg
     7381 aattcagaga gaccggccgg catcggcgct attttctccg ccagatgatg gccagcccca
     7441 ccacgagtgc aatcgccaca tagaccgcgg cctcgggtgc gcgaatgcgc gtgtgcggct
     7501 cgggcgcaaa gctcatctcg tacgcgcagg catcctgcga tcccgcgacg atctgcgctc
     7561 ccgcggcgat gacgcggccc tcgccgttga cgccgcgtat gcggacgacg gcttgatcga
     7621 gcttcaccga tggaaaaagg tcgtgtgcat cggcggagac gtgctcaaag cggtcgagga
     7681 agatccgctt ctctgcgcgc gtctggccat gcagatcttc gatcgagacc acgacggaga
     7741 gcgccgcgcc cgcggtctcc accagataga gcttgtaacg ggcgtcgagc gcgccatcgc
     7801 cgtgcagcaa tgcggactga ccgttgccga cgccgaggcg tgtggggatg gcggtgaggg
     7861 ccatggagac ggcggtcgag ggccccgcct cggggagata accgtagatt cgcgcggacg
     7921 cgacgatgtc ggcattcgct tcgatgcgca gggcgccgat gctgttgatg ctcgtcagaa
     7981 tcggcgggcc gaggggatcg aagacgtgcg tctcgcgcgg agcgagcagg accgtcgtct
     8041 gccgcggcga tggattggcg tggccactct cgagatacga caacgtcagc gtggctggat
     8101 gatcgtcggc gttcgtgatc cagagcgccg tgaggaagag acggcccgcg ccgccggctg
     8161 cacggccaac gaccggaacc acgaggtcat gatcagcaaa cgccgcggcg aggggatgcg
     8221 ccgccgccag gagaagaatg gggatgatga gtcttcgcac gtgatcagta tgcggcgcgc
     8281 gcggttacaa tcaatggttg agtggcaagg tgaccgctcg ggcttccgtg gccgcactcc
     8341 tttatcttct gtccgccgcc ggcgcgctcc ttctcgtgcg ccgcttcctg acgctttcgc
     8401 gtcacgtgtc ggcggtgttg attctccttc cactttgcct cacgggtaga gcgcttgtca
     8461 ccggaaacgt ctacgcgccg atcgatctgg cctatcactt tgatccgtta gcctcgatgt
     8521 cgacgaaggt tgggatcgag cagatcgcta atccgctgcc ctcggatgtg accgtgcagt
     8581 ttctaccctg gaacgcggca ttgcgttggg cgatcgcgca ccgccaatgg ccgctctgga
     8641 atccgttcga gctcggaggg aacgtgctgg ccgcggcggc gcaatcggct ccgttccatc
     8701 ccgtcacact cctcggcctt ctgcttccga tgccggacgc gctcggatac atggcctcga
     8761 tggcgtactt cctcgcggct ctggggatgt ttctcttcct gcgcaacctg gagcttcgtt
     8821 cgctgccgtc gctcttcggt gccgctggat ggatgctctc gacgtacgtg gtcacctaca
     8881 cgcacactgc gcacggcaac gcgatcgcgc tgctcccgtt cgtcatgctc ggcgcacgga
     8941 tggtggcgcg cagtccggga gtgcgcgcgg cgtcgctgct tgccggtgcg ctgatcctgt
     9001 tgacgctgtg cggtcatccg gagacctcac ttcacgtcat cgccctggcc acggtctatc
     9061 tgattttctg cgcgcgtgag aaatgggggc tcgtgatcat cagcggcctc ggcgccggga
     9121 tcgcagcgct gctcctcacc gcattctttc tgctgccgat gat
//
LOCUS       DATLVW010000069         3288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_581495, whole genome shotgun sequence.
ACCESSION   DATLVW010000069 DATLVW010000000
VERSION     DATLVW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581495"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="tilS"
                     /locus_tag="VNN25_02350"
     CDS             <1..300
                     /gene="tilS"
                     /locus_tag="VNN25_02350"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXH90394.1"
                     /translation="QLIQLAKGAKPTFTIRNRRHGDRFRPLGMSYDKKLKDLLIDRKI
                     AAASRDRIPLLIWQGAIVWVAGVEVSDLFKVTGEAADLYEVAIEKVHESLQRPGD"
     gene            260..775
                     /locus_tag="VNN25_02355"
     CDS             260..775
                     /locus_tag="VNN25_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXH90395.1"
                     /translation="MRKFTKVFSAREIEQRVAALAAEIRDDAGDAPLFLLGILKGASC
                     FLADLLRRIPGEAGYGFIDVVRDVADADTAAALEITYLSWIDIRDRNVYVVKDVVSTG
                     VIETYLMMQLRQKQPSTLKLVALLDRPDARTVDLTVDFRAFEASDGPYAGYGLEFEGR
                     CGNLPYIASVK"
     gene            954..2906
                     /gene="ftsH"
                     /locus_tag="VNN25_02360"
     CDS             954..2906
                     /gene="ftsH"
                     /locus_tag="VNN25_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXH90396.1"
                     /translation="MNSTLKTVLLWAVIIVLVVLLWSLFNTTKGTSETIAFSDFLDRV
                     NNGSVASVTIRGDDVHGETKQTAPGGKKEFQATIMANYPSMIDQLRAKGVKVQVEHAA
                     DAPFITALITWAPVLFLVGLWIFFMRQMQAGGNKALSFGKSKAKLLSGNAKKVTFKDV
                     AGVDEAKIELHEIIEFLKEPQKFTKLGGKIPKGVLLMGPPGTGKTLLARAIAGEANAP
                     FFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFESNDGVILVAATNRPDVLDPALLRPGRFDRRVVVDLPDLKGRE
                     GILRVHTRTIPLSEDVDPVVIARGSPGFSGADLANLVNEAALNAARYDKKKVQMIDFE
                     FAKDKVMMGVERKSMVMSDREKKNTAYHESGHTIVAAVLPAADPLHKVTIIPRGRALG
                     LTQQLPTEDKYSYSKRYLEAMLAVLMGGRLAEEIFIGEITTGAGNDIERASAMARRMV
                     CEWGMSDLGPVTFGKKEEAIFLGREFAQHQDFSEATAVEIDREVKRILDKAYAHAKEI
                     ITAHKSSLDRIARKLLERETLEGWEVNDILREETGTDYLKMKEYYPEASAGETMTIAP
                     GSGPSTDVSTPVQQPPAPAPVPDRTS"
     gene            3048..>3288
                     /locus_tag="VNN25_02365"
     CDS             3048..>3288
                     /locus_tag="VNN25_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HXH90397.1"
                     /translation="MTRIAVDVPEEIAKRLENAWPDVSRGALEAVALEGYRDGTLSRD
                     QVGELLGFSLWETEAFLKQRQAYLQYTEEDLALDRR"
BASE COUNT          677 a         1141 c          900 g          570 t
ORIGIN      
        1 caactcatcc aactagcgaa aggcgccaaa ccaaccttca ccatccgcaa ccgccgccac
       61 ggcgaccgct tccgtcccct cggcatgtcc tacgacaaaa agctgaagga tctgctgatt
      121 gatcgtaaga ttgccgctgc gtcgcgcgat cgcattcctc tcctcatctg gcagggcgcg
      181 atcgtctggg tggctggcgt cgaagtctcg gatctgttca aagtgacagg cgaggctgcc
      241 gacctttacg aggtcgcgat tgagaaagtt cacgaaagtc ttcagcgccc gggagattga
      301 acagcgcgtg gctgcgctgg cggcggagat tcgcgacgat gccggcgacg ctcccctctt
      361 cctcctcggc atcctcaagg gcgcatcgtg cttcctggcc gatctcctgc gccgcattcc
      421 cggcgaggcc ggctacggct tcatcgacgt cgtccgcgat gtcgccgacg ccgataccgc
      481 cgccgcgctt gagatcacgt atctgagctg gatcgacatc cgcgaccgga acgtctacgt
      541 cgtcaaagat gtcgtctcga ccggcgtcat cgagacgtat ctgatgatgc agcttcgcca
      601 gaaacagccg tcgaccttaa agctggtcgc cctcctcgac cgccccgacg ctcgcacggt
      661 cgacctgacc gtcgatttcc gcgccttcga ggcctccgac gggccctacg ccggctacgg
      721 cctcgagttt gaaggccgtt gcggaaacct gccgtacatt gcgtccgtta aatagaactt
      781 gttcgtagcg tcgttgggta tcgcccagaa acccacaaac catgcaaccc cacccacccc
      841 ccacacccta cgtcccacac cccgacccag aatttcacca cctgccgctc cgttcttccc
      901 acggcagtca atggcacgca cgaagcattc gcgtgctctc cttggaggtt tgattgaatt
      961 cgacgctcaa aacggtgctt ctctgggccg tcatcatcgt tctcgtggtt ctgctttgga
     1021 gcctcttcaa caccacgaaa ggaacgagtg aaaccatcgc cttcagcgat ttcctcgacc
     1081 gcgtgaacaa cggctccgtc gccagcgtga cgattcgcgg tgacgacgtt cacggcgaga
     1141 ccaagcagac cgcgcccggc ggcaagaaag agttccaggc cacgatcatg gccaactatc
     1201 catccatgat cgatcagctc cgcgccaaag gcgtgaaggt gcaggtcgaa cacgccgccg
     1261 acgccccgtt catcaccgca ctcatcacct gggctcccgt cctcttcctc gtcggcctct
     1321 ggatcttctt catgcggcag atgcaggccg gcggcaacaa agccctctcc ttcggcaaat
     1381 cgaaagcgaa actcctctcc ggcaacgcca agaaggtgac gttcaaagac gtcgccgggg
     1441 tcgatgaagc gaagatcgag ctgcacgaga tcatcgagtt cctcaaagag ccgcagaagt
     1501 tcaccaagct cggcggcaag atccccaaag gcgtcctgct gatgggacct cccggcaccg
     1561 gcaagacgct cctcgcgcgc gccatcgccg gcgaggccaa cgcccccttc ttctccatct
     1621 ccggctccga cttcgtggag atgttcgtcg gcgtcggcgc ctcccgcgtc cgcgaccttt
     1681 tcgagcaggg caagaagaac gccccctgca tcatcttcat cgatgagatc gacgccgtcg
     1741 gccgtcatcg cggcgccgga ctcggcggcg gtcacgacga gcgcgaacag acactcaacc
     1801 agctcctcgt cgagatggac ggcttcgaat ccaacgacgg cgtgatcctc gtcgccgcca
     1861 cgaaccgtcc cgacgtcctc gaccctgccc tgcttcgtcc gggccgcttc gaccgccgcg
     1921 tcgtcgtcga cctccccgac ctcaaaggcc gcgagggcat cctgcgcgtg cacacgcgaa
     1981 cgattccgct gtccgaggat gtcgatccgg tcgtgatcgc acggggctca cccggattca
     2041 gtggcgctga cctcgccaac ctcgtcaacg aagccgccct aaacgccgcc cgttacgaca
     2101 agaagaaagt ccagatgatc gacttcgagt tcgcgaagga caaggtcatg atgggcgtgg
     2161 agcgcaagtc gatggtgatg agcgaccgtg agaagaagaa cacggcctat cacgagtcag
     2221 gacacacgat cgtcgccgcg gttctccctg ccgccgatcc gctgcacaaa gtcacgatca
     2281 tcccccgcgg ccgcgccctc ggcctcacac aacagctccc caccgaggac aagtactcgt
     2341 actcgaagcg ctacctcgag gcgatgctgg ccgtgctgat gggcggccgc cttgccgagg
     2401 agattttcat cggcgagatc accacgggtg ccggcaacga catcgagcgc gcctcggcca
     2461 tggcccgccg catggtctgc gaatggggca tgtccgacct cggccccgta accttcggca
     2521 agaaagagga agccatcttc ctcggccgcg agttcgccca acaccaggac ttctccgaag
     2581 ccaccgccgt cgagatcgac cgcgaagtca aacgcatcct cgacaaagcc tacgcgcacg
     2641 ctaaagaaat catcaccgcc cacaaatcgt ccctcgaccg catcgccaga aaactcctcg
     2701 agcgcgagac gctggaagga tgggaagtca acgacatcct ccgcgaagaa actggcaccg
     2761 actacttgaa gatgaaggag tactaccccg aagccagcgc cggcgaaaca atgacgattg
     2821 cgcccggctc gggcccatcg acagacgttt ccacgccggt gcaacagccg cccgcaccag
     2881 cgccagtacc cgatcgcacg tcgtagcttc actccaccca caccgcaatg tcagggccgc
     2941 cgcaaggcgg ccctttccat tggaacgccg gcgcgcaatt acgctccctc tcccccgtgc
     3001 caccgtcctc agagcaccag cgccttcagg tgcgataaga ttgcatggtg actagaattg
     3061 ctgtcgatgt acccgaggaa attgcaaagc ggctcgagaa cgcctggcct gacgtctccc
     3121 gtggcgcact cgaagctgtg gcgttggaag ggtatcgcga cggcacgttg agccgcgacc
     3181 aggttggaga actcctaggc ttctcgttat gggaaacgga ggcgtttctc aagcagcgcc
     3241 aagcctatct ccagtacacg gaggaggatc tcgcgctcga ccgcagag
//
LOCUS       DATLVW010000070         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_335339, whole genome shotgun sequence.
ACCESSION   DATLVW010000070 DATLVW010000000
VERSION     DATLVW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335339"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..973
                     /locus_tag="VNN25_02370"
     CDS             20..973
                     /locus_tag="VNN25_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH90398.1"
                     /translation="MSATVSSTFSRRFRRVAAALAASLALGALSAHAAPTPFPADFRS
                     SQVVNADARINVRVGGHGPAVVLIHGFGETGDMWSPMAAELAKDHTVVVPDLRGMGLS
                     SHPAGGYDKWTQAGDIRAVLDKLGIDRADIVAHDIGTMVAYAYAARYPDKTSRLVVMD
                     SPIPGIPPWNQIVRLPALWHFNFGGPDAERLVAGRERIYLDRFWNEFAGDPSKIDEAT
                     RRHYAAIYARPGAMHSAFAQFLAIGQKDEADNLKAMETKLTMPVLAIGAEKAFGANVA
                     VVMRNAATNVQQVVVPNAGHWLMEEAPAVTIAAVQNFLAAR"
     gene            complement(1013..>2095)
                     /locus_tag="VNN25_02375"
     CDS             complement(1013..>2095)
                     /locus_tag="VNN25_02375"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HXH90399.1"
                     /translation="VVNSHLVPTAAFQSNPDIDFRAAELHQAIARSLGSCESERIDFT
                     LAATTLLGDTVAANLLLVGFALQRGWLPVGAPAVERAIELNGTAVELNRRALKLGRLA
                     AHAPERLAALILEAAAARRATPVPMVPDGEGATEARIRVREAFLAEYQDRTYAQRYRS
                     LIDRVATREQIVVPGSKALTDAVARGYFKLLAYKDEYEIARLHATTLAGQLDATFSGA
                     AKLSFHLAPPGVARPDPQTGQIRKIELGSWILPVFRLLAKLKFLRGTVFDPFGHTAER
                     KMERRLITEYEDLVEHMMQTLCSERLDLAVELAKLPDGIRGFGHVKDRHVAAVKKRET
                     ELRQQWDRGRMDIIHEGVHQPIQSPK"
BASE COUNT          359 a          709 c          664 g          363 t
ORIGIN      
        1 agacaccaag gagccaagca tgtccgcgac cgtttcgtcc acgttctccc gccgcttccg
       61 ccgagtcgcg gccgccctcg ccgccagcct ggcgctgggc gccctgtcgg cgcacgccgc
      121 gcccacgccc ttcccggccg acttccgcag ctctcaggtg gtgaacgcgg acgcgaggat
      181 caacgtccgc gttggcggcc acgggcccgc ggtcgtgctg atccacggct tcggcgagac
      241 cggcgacatg tggtcgccga tggccgccga gctcgcgaag gaccacaccg tcgtcgtgcc
      301 cgacttgcgc ggcatgggcc tgtcctcgca ccctgccggc ggctacgaca agtggacgca
      361 ggccggcgac atccgcgccg tcctcgacaa gctcggcatc gaccgcgccg acatcgtggc
      421 ccatgatatc ggcacaatgg tcgcgtatgc ctacgcggcg cgctatccag acaagacgag
      481 ccgcctggtc gtgatggact cgccgatacc cggcatccca ccgtggaacc agatcgtgcg
      541 cctgcccgcg ttgtggcact tcaacttcgg cggcccggac gccgagcgcc tggtcgccgg
      601 ccgcgagcgc atctacctcg accgcttctg gaacgagttc gccggcgacc cgtcgaagat
      661 cgacgaagcg acgcgccgcc actacgccgc gatctacgcc aggcccggcg cgatgcactc
      721 cgcgttcgcc cagttcctcg cgatcggcca gaaggacgag gccgacaacc tcaaggcgat
      781 ggagacgaag ctgacgatgc cggtgctcgc gatcggcgcc gagaaagcct tcggcgccaa
      841 cgtggcggtc gtcatgcgca acgcggcgac gaacgtgcag caagtcgtcg tgccgaacgc
      901 cggccactgg ctgatggagg aagcgcccgc ggtgacgatc gccgctgtgc agaacttcct
      961 cgcagcacgc tgacgggtgg cctcaagatt cggatcgttt cgtacgccag gatcacttgg
     1021 gagattgaat tggctggtgg accccttcat ggatgatgtc cattcgtcct cggtcccatt
     1081 gctgccgtaa ctccgtctcg cgtttcttta ccgctgcaac atgtcggtcc ttgacgtgtc
     1141 cgaatccacg gattccgtct ggcaattttg cgagctccac ggcaagatcc agccgttccg
     1201 agcaaagagt ctgcatcatg tgttcgacca gatcctcata ctcggtgatc aagcgacgtt
     1261 ccattttgcg ttccgccgta tggccgaacg ggtcgaagac cgtgccccgt aggaatttta
     1321 atttcgcgag caagcggaac acgggaagaa tccacgaccc cagttcgatc tttcgaatct
     1381 ggcctgtttg cggatccgga cgggcgactc ccggcggcgc caggtgaaac gacagctttg
     1441 ccgcgcctga gaacgttgcg tcgagctgcc ccgccagcgt cgtcgcgtgc aaacgcgcga
     1501 tctcgtactc gtccttgtag gcgagaagtt tgaaatagcc tcgggcgact gcgtctgtga
     1561 gggccttcga gccgggcacc actatttgct cacgggtggc gacccggtcg atgagcgaac
     1621 gatagcgctg cgcgtaggtt cgatcttggt actcggccaa gaacgcctcc cgtactcgaa
     1681 tccgcgcctc ggtggcgccc tcgccgtccg ggaccattgg caccggggtc gcacggcgag
     1741 cggcggcggc ttccagaatc agcgcggcga gccgttcggg ggcgtgcgcg gccaacctac
     1801 cgagcttcag ggcgcgacga ttcaactcca cggcggttcc attgagttcg atggcccgct
     1861 cgaccgcggg tgcgccaacg gggagccagc cgcgttgcag ggcaaacccc acgagaagca
     1921 ggttcgccgc caccgtatcc ccgagcagcg ttgtcgccgc tagtgtgaaa tcgatgcgct
     1981 ccgactcgca cgagccgagc gaccgagcta tcgcctggtg cagctccgcg gctcggaaat
     2041 cgatgtcggg gttcgactgg aatgccgcgg tcggaacgag gtgcgaattg accac
//
LOCUS       DATLVW010000071        13924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_85317, whole genome shotgun sequence.
ACCESSION   DATLVW010000071 DATLVW010000000
VERSION     DATLVW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85317"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VNN25_02380"
     CDS             <1..1774
                     /locus_tag="VNN25_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH90400.1"
                     /translation="DGRIPNLRALAAGGTATSLQTIQPTVSPMVWTTVATGLTPDRHG
                     VIDFMDRGRHSPVDAYSRRVPALWDVADAFGRPALVANWWSGWPATAPNSIFYGTPVE
                     DVANAVYPSSLGDRATSLAVPVSTVGYEQAHRFLNITPDEWNTAIDANNPSDPIVIFR
                     SILAKTWNDHRVAINLYNDQRKNGQDPMLVMVSYEGTDAVNHLFSPYHPPYRDGISEQ
                     NYRKFWPAVSNYYAEVDRLIGEWMNVLPHDTTVMVMSSYGFRWGKNRPRSMPNGGAAL
                     SDHRNPGMFIAYGPHVIPTRLHPMSVYDVAPTVLSLLGLPQSIEMPGHAATWALKDIT
                     PITSVRVVSYGEFVAAKPVGTSATIAEAPYEAQLQAIGHLNDPTRNLTPVLEDEGDAK
                     RAATPLSPERWGQYAYYNNLGVELRAKGKLSEAIDAFDRAIDINPTRPTPHLNEAMVF
                     FDRQQFAPADAQILNAIALGLPNAESYLIDFAALYRQRNMNARAIALLYQGRKLFPQS
                     YVIAVNLGSALLEAERYTEGVPELVRALGLQPSSTLALNNLGLFYAKQNDYGRALDFW
                     NRSLSVDAHQPQIREAAVAARSRL"
     gene            complement(1913..2437)
                     /locus_tag="VNN25_02385"
     CDS             complement(1913..2437)
                     /locus_tag="VNN25_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90401.1"
                     /translation="MVGIWLGTQLRLSRKDDQQSSRGEISCQTGGEGLSKGEEQASAL
                     RGLHDKENLAEAIDLPLLRLACHCFDKHAVRFDIHVACLDIHSVSFDIQAALPRHSER
                     ENVEADILKVEAQLLNVEADVWNVEAQLLNVEPDFSNVEAEVLNVEAAVLNVEAQFLN
                     VEAEICMFRQKMEC"
     gene            2427..2933
                     /locus_tag="VNN25_02390"
     CDS             2427..2933
                     /locus_tag="VNN25_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXH90402.1"
                     /translation="MPTIEREIGRFQDFLARQGQKLTQERTALVREIFATHYHFEADE
                     LLFKMKQKSTKISRATVYRTLELLVKSGLVRRVHLGEDHYHYEHVRGDSHHDHLVCTA
                     CGSVIEFNDPELEQRQLEICARKKFTPTFHNLQILGVCDSCRRKGEQPASPDRVTSIG
                     EGAMRLRK"
     gene            2943..3701
                     /locus_tag="VNN25_02395"
     CDS             2943..3701
                     /locus_tag="VNN25_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HXH90403.1"
                     /translation="MIPALIIVFREGFEAFLTVAIIFAFLRKTGRDWLRPAVYAGIAT
                     SIAASFALGYALMRISEHQSLIEGILGVVAAVLVASFVVHVWRVAPRMKGDMERRLDS
                     YTSATSRFLAIAGVFFFTVLMVTREGMETALMLIQVRNSRFILGSLLGIAAAAAMSWA
                     WAHYGHRIDIKRFFQVTGLFLLLFTIQILFYSIHEFSEAEVLGRWSEAIHNATEPYSP
                     VGIYGKWISVAMVGLCAAWLVGVSMKDRFERSRA"
     gene            3755..4150
                     /locus_tag="VNN25_02400"
     CDS             3755..4150
                     /locus_tag="VNN25_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90404.1"
                     /translation="MPEFLLAHLGIPFLAGLVFLFMTAASDAHPLSLKSCNEVAVDFT
                     VLSIGATGAIFLNPRLVAHWKELTGVYGIVVVLVNLLLASFLVYRRRWRQETPTKMQA
                     YVDLFLGVLALTVTLMAFYAGFTGSVQKT"
     gene            complement(4354..5748)
                     /gene="gltX"
                     /locus_tag="VNN25_02405"
     CDS             complement(4354..5748)
                     /gene="gltX"
                     /locus_tag="VNN25_02405"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXH90405.1"
                     /translation="MKLRFAPSPTGFLHVGGARTAIFNLLHARRHGGTMLLRIEDTDV
                     ERSRQRHAEQIISSLQWLGVEWDEGPVYQSDRLDRYRERVEELVVSGQAYRCYCTVEE
                     LEAERAAAEKAGKQWRYSGRCRASEGDPAEPHVIRFAVSSGTIEFHDLIRGDVQFEGD
                     LIDDFVLLRSDGNPTYHLSVVVDDIDMEITHVARGDDHLSNTPKHVLLFRAFGAAVPS
                     FAHLPLILGSDKKRLSKRTGATSAEEYREMGIVPDALFNFLTLLGWSPGENREIFTRD
                     DAAHWFDLSHVNKAPAVFDPEKLLWMNGQYLMRMSAEQIYPHLLPFLGDGAKTLDDLR
                     TIIDLHKMRARTLREIAEQMAVYFVGDDAIEYDPDVVKKHIKGDDLSTRLSTLHDALA
                     STEPFDVATSEAALRKVAEEQGVSAAKLIHPLRLALTGKGASPPIFDVAVVLGKERSL
                     RRLQRLIERIPKLD"
     gene            5959..6351
                     /locus_tag="VNN25_02410"
     CDS             5959..6351
                     /locus_tag="VNN25_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH90406.1"
                     /translation="MHRSRGFSLAEVLVALAILAIVITTTIAMFAERTKRMREASETI
                     IAYQALSNEAEYWRRLDFAAIDDPAQQKFQSPDLSVLAPLAPYSTSVKVETPQPDVRN
                     VTLTIRWATNREARLAIARANTGGTGLW"
     gene            6345..6860
                     /locus_tag="VNN25_02415"
     CDS             6345..6860
                     /locus_tag="VNN25_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH90407.1"
                     /translation="MVRRDRSRGFTLMEVVVATALFGVFLFIIVTLTSEMRRNEKKWP
                     VDFFSNPEVGSVLARLRRDVYDSTSMPGSFDVYTASPTTLILYTISVDRSSAETVVWD
                     FSKPGEAHRKAYRVNQLSSEWVARSVPVFICPSCLTGESVNGEPAVHIQAVDKEGKLA
                     IDEIFVPRPHA"
     gene            6820..7248
                     /locus_tag="VNN25_02420"
     CDS             6820..7248
                     /locus_tag="VNN25_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90408.1"
                     /translation="MPSTRSSFRARTRERGYVLITAIALAILYFALMELMLIDSSRAL
                     REAQRFRSKVIATTLAESAAELSAASMVTRNAGNDINASDEQGSMKASCTVTGTANGT
                     AFNIDAEATTSGVAPLTSKVSVQGRISQSRVIVDYTYHSQ"
     gene            complement(7371..7652)
                     /locus_tag="VNN25_02425"
     CDS             complement(7371..7652)
                     /locus_tag="VNN25_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90409.1"
                     /translation="MIDNMNPTSQFHPYQRETSVPVAERPLTGLDSVLDKIGIDPTSV
                     RSVRDQMRNLKVNDSFGKVRAYAKANPGKMLGGLAALAIGLGVMRARRA"
     gene            complement(7718..8299)
                     /locus_tag="VNN25_02430"
     CDS             complement(7718..8299)
                     /locus_tag="VNN25_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXH90410.1"
                     /translation="MQTKAATIPSALLTRLVRGRAALDVLLVIGATVVIALAAQVAIP
                     LPFSPVPLTLQPLAVLLVGVTLGSVRGAAAAALYLLEGFSGLPVFALGHAGPMWLAGP
                     TAGYLLSYPCAAWVAGWVSERGWGSTTLRAVAGMLAALGVIYLGGFSWLAVLSGSRAA
                     WIGGVRPFILADIVKVAIAASLLPQMQRLIAKI"
     gene            complement(8400..10961)
                     /locus_tag="VNN25_02435"
     CDS             complement(8400..10961)
                     /locus_tag="VNN25_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXH90411.1"
                     /translation="MTDRILDALARLCLRRSKEILIAGLVLAVLAVAGATRLSFDPDL
                     LNLIPQQNRQVNEFRKVLKDLGTIDYHIVVIDMPKGRDVHEYDSLIDAIAEGYRKNPK
                     IEDVTYKIPNPLDFIEIILPKALLFLSPAELDEVAAKLTDEGIRASVARNHAMLQTPQ
                     ALAMKPLVQYDPFNLVPVFLRKFQSAGSGFKIDTSSGYYMSSDHSMLLILTKPRRPAQ
                     DVPFGKELLSEGTLLEAQALKEFQKTAEPGTPLPKVSHTGGYEIAVGDADLIRQDVII
                     NIIGSVVGVLALFLYAFRRLASVIYAGIPLALGLLLTFGMAGVTYGKLSQASAGFAAL
                     LAGLGIDFITVLYGRYVDERNRGASMQDAIRVIMRSTMPGVFVAAITTAGTFFAFLAT
                     DFRGMTQLGLLTGVGIVFFLLCVMFLLPALIVFSERKQARRAPKLYLHSFGAGKLIDI
                     SVARPGITIGIWVVFVVACGVLATRVKFSDNIQDLRAKGNTGVLNQTRITEKFGQSFD
                     FMMYVCEGKTVEEALEKTRAAAEDLDGLVRDHTIASYQSISSFLPPAVDQQKIIDRLR
                     AGSGNEFNFARVDHTFRAALAENGFRPEAYDDYMKLFAQTLQPAQPVTIQTIGNKDIE
                     KLTTRFIKPVNGGWMSVINVYPTGGKWGRDVSPKLLAVPDRHPGDILTGVNLVSATLR
                     RIVKADATRSTIIGFIAVFVLMFLSFRTVKLTALSFVPFVAGAVGMLGLMALLDLQFN
                     FMNIFVGLMIIGVATDYAIYMLQRYREDVAAFRGSAHETGKAIVMAALTAIVGYGSFA
                     FSHYPGLRSIGYASTFGIGLSGLAAITLLPAILVMKKAEIRDKRSEIKTTFSSDL"
     gene            11014..11604
                     /locus_tag="VNN25_02440"
     CDS             11014..11604
                     /locus_tag="VNN25_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90412.1"
                     /translation="MRSQISQVAFLMVLLVAACQKAEPLTAAKAEQILASSRFAAEPI
                     YAEVPQKVWWNAHFPKDEFDERSLRTFDNLTKAGLITVTGGPTPDGAAYVAKTTQKGF
                     PVLGTAPSARGPVFRGLICYKVYDGLRNFQRHPTEPTTGHGELVWHYTKPTTLYPMFE
                     TKQNKPLDKPFLSLVSFYYKDHQWQFDVTVRKTEAE"
     gene            12116..>13924
                     /locus_tag="VNN25_02445"
     CDS             12116..>13924
                     /locus_tag="VNN25_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH90413.1"
                     /translation="MNDSVSRENISSVAHQMAAQHGGSIQRIWQDALKGYFAVMTEAQ
                     AQALSHNPNVKYVEENAAMFTSSRVPTNVDPACDYPSNCTTADNRLWHLDVLDQNDAV
                     GTHDYAYCTDGSGVYVYVVDFGVMRAHREFNNDPNRVADGYNASGDCLNKDSHGHCLA
                     SLDYYPAYDPCHGSPTAADQDMGMEVFGNSHGTGVGSLVAGRNIGVAKNATIVPVKVA
                     RCGEFSSKLIQRNTTYPPGVIVRSNQGFYQTLNTGTTGPGVPNPCCSPDGSLNLQFLG
                     NDGLTNFGTVQMIIEGLDWILRPVEQGGNPYPKSPAVVTLSTYRVVGTDGVTNTPLGS
                     SLSFEDAIANLLKYNNGQGITVIASANNQDANACDTSPGRMSRNNPNNPNDPNHPYKV
                     ITAGGTMLRNNPDSNPATGGSNVNLPEPTYDSSKATRIARWRCNAGDSDGCSANIYAQ
                     PPPTAPNPATNSSAYVGWTLGSNGGQCVTLFAPAKNIPVATIAADAGGNPSYRDSRAT
                     NGLASGTSWSAPFVAGVVARILQNNPGYSVDQVYSALMSNTTPDLDPIELDPPGVTGT
                     PNALLHIPDVSIAPLPATITGPFTASASGTAPLTYQW"
BASE COUNT         2798 a         4328 c         4121 g         2677 t
ORIGIN      
        1 cgacggccgc atcccgaacc tcagggcgct ggccgcaggg ggcacggcga cgtcgctcca
       61 gaccattcag ccgacggtct cgccgatggt ctggacgaca gtcgcgaccg gcttgacgcc
      121 tgatcgtcat ggtgtgatcg attttatgga ccgcggccgg cattcgccgg tggacgcgta
      181 ttcgcgccgc gtgccggcgc tttgggatgt ggcggatgca ttcggtcgtc ctgctctcgt
      241 cgccaactgg tggtcaggct ggccagccac ggcaccgaac agcatcttct atggcacacc
      301 ggtcgaggat gttgccaacg ctgtctatcc ttcgtcgctt ggcgatcgcg cgacgtcgct
      361 ggccgtgccc gtcagtaccg tcggctacga acaagcgcac cgctttctca acatcacgcc
      421 cgatgagtgg aacacggcta tcgacgcaaa caacccgtcg gatccgatcg tcatcttccg
      481 gtcgatcctg gcgaagacgt ggaacgacca ccgcgtggcc atcaatctct acaacgatca
      541 gcggaagaac ggtcaggatc cgatgctggt gatggtctcg tacgaaggga ccgacgcggt
      601 caaccatctc ttctcgccct accacccgcc ctaccgggac ggcatcagcg agcagaacta
      661 ccgcaagttc tggccggccg tttcgaatta ctacgccgag gtcgatcggc tcatcggcga
      721 atggatgaac gttctgcccc atgacaccac ggtcatggtc atgtcgtcgt acgggtttcg
      781 ctgggggaag aaccggccgc gctcgatgcc gaacggcggc gcggcgttat cggatcaccg
      841 aaatcccggg atgttcattg cttacggtcc gcacgtgatc cctacccgat tgcacccgat
      901 gtcggtttac gacgtcgctc caacggtgct gtccctcctc ggcctaccgc agtcgatcga
      961 gatgcctgga cacgcggcga cgtgggcact caaagacatc acgccaatca cgtctgtccg
     1021 cgtcgtcagc tatggtgaat tcgtcgcggc gaaaccggtg gggacgtcag ccaccatcgc
     1081 cgaggcgccg tacgaagcgc aactgcaggc gattggccac ctcaacgatc ccacgcgcaa
     1141 tctgactccc gttctcgagg acgagggtga cgcaaagaga gcggcgacgc cgctgtcgcc
     1201 ggagcgttgg ggacaatacg cgtactacaa caacctcggc gttgagttgc gcgccaaggg
     1261 gaagctgagc gaggccatcg acgcattcga ccgcgccatc gacatcaatc cgacgcgccc
     1321 gacgcctcat ctcaacgagg cgatggtgtt cttcgaccgg cagcaatttg ccccagccga
     1381 cgcgcagatc ctgaacgcaa ttgcgcttgg attgccgaac gcggaaagtt atctcatcga
     1441 ctttgcggcg ctctaccggc agcgcaacat gaacgcccgc gcgatcgcgt tgctgtatca
     1501 gggccgcaag ctcttcccgc agtcgtacgt catcgccgtg aacctcgggt ccgcgctact
     1561 ggaggccgag cgctacacgg agggcgtgcc ggagctcgta cgcgcactcg ggctccagcc
     1621 gtcctccacg ctggctttga acaacctcgg cctgttttac gcgaaacaga acgactacgg
     1681 tcgcgctctc gacttctgga accgctcgct ctcggtcgac gcccatcaac cgcagattcg
     1741 ggaggcggcg gttgcggcgc ggtcgcggtt gtaggggtgc agggatgtcg gccggtgaca
     1801 atgggaagag gtgccacgaa aacgtacgcc gaggcgagga cgactgcacc tctttccggg
     1861 acgccagtct cgatattggt aacgtttccc cgaaacaggc aagagttgcg cttcaacatt
     1921 ccattttctg cctaaacatg cagatttctg cctcgacatt aagaaattgg gcttcaacat
     1981 tcagaaccgc tgcttcgaca ttcaaaacct cagcctcaac attcgaaaag tcaggttcga
     2041 cattcagaag ttgtgcttcg acattccaaa cgtcagcttc gacattcaga agttgtgctt
     2101 caactttcaa aatgtctgct tcgacattct cccgttctga atgtcgaggc aaggcggcct
     2161 gaatgtcgaa gctgacggag tgaatgtcga ggcaggcgac gtgaatgtcg aagcggacgg
     2221 catgcttatc aaagcagtgg catgccaatc gaagcaaagg cagatcgatt gcttcggcaa
     2281 ggttctcctt gtcgtggaga cctcggagcg ccgacgcctg ctcctctcct ttcgacagcc
     2341 cttcgcctcc cgtttgacac gaaatttctc cgcggctaga ctgttgatcg tctttgcgac
     2401 tgagtctcag ttgcgtaccc aaccaaatgc ccaccatcga acgcgagatc ggccgcttcc
     2461 aggacttcct ggccaggcag ggacagaagc tgacgcagga gcgtaccgca ctcgttcgcg
     2521 agatcttcgc gacgcactac cacttcgagg ccgatgaact gctcttcaag atgaagcaga
     2581 agagcaccaa gatctcccgc gccaccgtct accgcacgct ggaactgctc gtaaagtccg
     2641 gactcgtccg ccgggtgcac ctcggcgaag atcactacca ctacgagcac gtccgcggcg
     2701 attcccacca cgatcatctc gtctgcaccg cctgtggttc cgtcatcgag ttcaacgatc
     2761 cggagctgga gcagcgccag ctcgagatct gcgcccgcaa gaagttcacc ccgacgtttc
     2821 acaatctcca gatcctcggc gtctgtgact cctgccgccg gaaaggggag cagcccgcct
     2881 cacccgatcg cgtcacgtcg atcggcgaag gggccatgcg gctccgaaaa taaacccact
     2941 ccatgatccc tgccctcatc atcgttttcc gcgaaggctt cgaggccttt ctcacggtcg
     3001 ccatcatctt cgcgttcctc cgcaagaccg gccgcgactg gctgcggccc gcggtctacg
     3061 ccggaatcgc cacctccatc gccgccagct tcgccctcgg ctacgcattg atgcggatca
     3121 gcgagcatca gtcactgatc gaaggcattc tcggcgtcgt cgccgcagtg ctcgtggcct
     3181 cattcgttgt tcacgtatgg cgtgtcgctc cgcggatgaa gggcgacatg gaacgccggc
     3241 tcgacagcta cacctccgcc acttcacgct tcctcgcgat cgccggcgtc ttctttttca
     3301 cggtactgat ggtgacgcgc gagggaatgg agacggcgct aatgctgatc caggtccgca
     3361 atagccgttt cattctcggc tcactcctcg gcattgccgc cgccgccgcg atgtcatggg
     3421 cgtgggcgca ctacggccac cgtatcgaca tcaaacgctt cttccaggtg accggcctct
     3481 tcctgctgct cttcacgatc cagatcctct tctattcgat tcacgaattc tcagaagcgg
     3541 aagtcctcgg ccgatggagt gaagccatcc acaacgccac cgagccgtat tctccagtcg
     3601 gcatctacgg aaaatggatc tccgtcgcga tggtcggcct ctgcgcggcg tggctcgtcg
     3661 gtgtttcgat gaaggaccgg ttcgagcggt cacgagcgta gtaatatcgg cgcatcgtga
     3721 tagcctaagg ctatacatcc ttcgggcgat aacgatgcca gagttcttgc ttgctcatct
     3781 gggaattcct ttcctggccg gattggtctt cctttttatg acggctgcgt ctgacgcaca
     3841 tccgctgtcc ctcaagagct gcaatgaggt cgcggttgac tttaccgtgc tcagtattgg
     3901 cgcgactggc gcgatcttct tgaatcctcg cttagtagct cactggaaag aacttaccgg
     3961 cgtgtacggt atcgttgtgg tattggtcaa ccttttgctt gccagctttc tcgtgtaccg
     4021 tcgtcgctgg cgacaggaga cgccgacaaa gatgcaggcg tatgtcgacc tctttcttgg
     4081 cgtgcttgct ctcaccgtaa cgctgatggc tttctacgcc ggcttcacag gttcggtaca
     4141 aaagacctaa tggagagaac atggactttc taaaatttgt ggctacggcg gtgctctcct
     4201 cagcgctcgt atcggctgtt ctttttgcga tcatttcaaa gctgacgcca agaagtcacc
     4261 gaactaacgc gacacctgat gccgagccaa cgactgccaa agtcgagatc cgcgagagac
     4321 ctcgcattgc ggtttagcgg gctctgagca cgattaatcc aacttcggta tccgctcgat
     4381 caaccgctgc aaccttcgca gcgaccgctc cttccccagc accacggcaa cgtcaaagat
     4441 cggcggcgac gctcctttgc ccgtgagcgc gagccgcagc ggatggatga gtttcgcggc
     4501 gctgacaccc tgctcttcgg caactttgcg cagtgcggcc tccgaggttg ctacgtcgaa
     4561 cggctcagtt gaggccagcg cgtcgtgcag cgtcgaaaga cgggtgctga gatcgtcgcc
     4621 ctttatgtgt ttcttcacga cgtcggggtc gtactcgatg gcgtcgtcgc cgacgaagta
     4681 gacggccatc tgctcggcga tctcgcgaag tgtccgtgca cgcatcttgt ggagatcgat
     4741 gatcgtccgc agatcgtcga gcgtcttcgc tccgtcgcca agaaacggca aaaggtgcgg
     4801 atagatctgc tcggcggaca tgcgcatcag gtactggccg ttcatccaga gcagcttttc
     4861 cggatcgaag acggcgggcg ccttgttgac gtgcgacaga tcgaaccagt gcgcggcgtc
     4921 atcacgcgtg aagatctcgc ggttctcgcc gggcgaccag cccagcaggg tcaggaagtt
     4981 gaagagcgca tcgggaacga tgcccatctc gcggtactcc tcggccgagg ttgcgccggt
     5041 gcgcttggaa agccgcttct tgtcgctgcc aaggatgaga ggaaggtgcg caaatgacgg
     5101 cacggcggcg ccgaacgcgc ggaagagcag cacgtgtttc ggcgtgttcg agaggtgatc
     5161 gtcgccgcgc gcaacgtgcg tgatttccat gtcgatgtcg tcgacgacta cggagaggtg
     5221 ataggtggga ttgccgtcgg aacggaggag gacgaaatcg tcgatcaggt cgccctcgaa
     5281 ctgcacgtcg ccgcggatga ggtcatggaa ctcgatcgtt ccggacgaca ccgcaaaacg
     5341 aatgacatgc ggctccgcag gatcgccctc ggatgcccgg caacgtccgg agtagcgcca
     5401 ttgcttgccg gccttttccg cggccgcccg ctcggcttcg agctcctcga ccgtgcaata
     5461 gcatcgatac gcttgcccgc tgacgaccag ctcctcgaca cgctcgcgat aacgatccag
     5521 ccgatcgctc tgatagaccg gcccttcatc ccactccacg ccaagccact gcagcgagct
     5581 gatgatctgc tccgcatgac gctgacggga tcgctcgacg tccgtgtcct cgatgcgcag
     5641 cagcatcgtg ccgccatgac gacgggcgtg aaggagattg aaaatcgcgg tgcgggcgcc
     5701 cccgacgtgg aggaaaccgg tggggctggg agcgaaacgg agcttcatgg ggttgatgat
     5761 agctgttagc ttcggaagag atcagagaca agagattaga gatcagagat cagagatcag
     5821 agagaggtta tcgaagggtc agccgcgaac ctggcctctt gtctctgatc tcttgtctct
     5881 gatctcttgt ctctgatctc ctgcatctga tctctcatct ctgatccccc agcctcccag
     5941 cgcgtagaat caaaggccgt gcaccgctcg cgtggtttct cgcttgctga ggttctggtc
     6001 gcgttggcga ttctggcgat cgtgatcacg accaccatcg ccatgtttgc ggagcggacg
     6061 aaacggatgc gcgaggccag cgagacgatc atcgcgtatc aggctctgtc gaacgaggcc
     6121 gagtattggc ggcggttgga ctttgccgcg atcgatgatc cggcgcagca gaagttccaa
     6181 tctcctgacc tctccgtgct tgctccgctg gctccgtaca gcacgtccgt caaggtcgaa
     6241 acgccgcagc cggatgtccg caacgtcacg ctgacgattc ggtgggcgac gaaccgggag
     6301 gcccgcctgg cgatcgcacg ggcgaataca ggtgggactg gcctatggtg agacgtgacc
     6361 gctcacgcgg attcacgctg atggaggtcg tggtcgcgac ggcgctcttc ggtgttttcc
     6421 tcttcattat cgtcacgctt acatcggaga tgcgccgcaa cgagaagaag tggccggtcg
     6481 atttcttctc gaatccggaa gtcggcagcg tgctggcgcg cctccgtcgc gatgtctacg
     6541 acagcacctc gatgcctggc agctttgatg tctacacggc ctcgcccacg acgctgatcc
     6601 tctacacgat cagcgtcgac cgctcttcgg ccgagaccgt cgtgtgggac ttcagcaaac
     6661 cgggcgaggc gcaccgcaag gcgtaccggg tgaaccaact gtcgtccgaa tgggtggcgc
     6721 gcagcgtacc ggtcttcatc tgtccgtcgt gcctgaccgg agaatcggtc aacggcgagc
     6781 ccgcggtgca tatccaggca gtcgacaagg aggggaagct tgccatcgac gagatcttcg
     6841 ttccgcgccc gcacgcgtga gcgcggctac gtgctgatca ccgcgatcgc gctggcgatc
     6901 ctctacttcg ctctgatgga gttgatgctg atcgactcct cacgcgcgct gcgcgaggcc
     6961 cagcgtttcc gctcgaaggt catcgccaca acgctcgccg aaagcgcggc cgagctttcg
     7021 gcggcgtcga tggtcacgcg caatgccggg aacgacatca acgcgagcga cgagcaggga
     7081 tcgatgaagg cgagttgcac cgtcactgga acagcgaacg gaaccgcgtt caacatcgat
     7141 gcggaagcaa cgacgagcgg cgtggcaccg ctgacgtcga aggttagcgt acagggcagg
     7201 atcagccaat cgcgcgtgat cgtggattac acgtatcact cgcagtgagg gtgtcgctga
     7261 tcgggggtcg ggagtcggag cgaacccggg accagcgaga gtttgacggg agtcgatcaa
     7321 atcggggttt ctgttctaac tccgacacgc gacccccgac ccccgacgtc ctacgctctc
     7381 cgcgcccgca tcacccccaa accaatcgca agcgcagcaa gtcctcccag catctttccc
     7441 ggattcgctt tcgcgtacgc ccggaccttg ccgaacgaat cattcacctt gagatttctc
     7501 atctgatcgc ggacggagcg gacgctcgtc ggatcgatgc cgatcttgtc cagaacgctg
     7561 tccagcccgg tcagcggacg ctccgcgaca ggtacgctcg tctcgcgctg ataggggtgg
     7621 aactgactgg tgggattcat gttgtcgatc attcacatcc tcctcgtacg gttatttaca
     7681 ctgcgaggag ggtgccacga acggcgtgca acgaccgtca gatctttgcg atcagccgct
     7741 gcatctgcgg aagcagcgac gctgcgatgg cgaccttcac gatgtccgcg aggatgaacg
     7801 gccgcacgcc gccgatccac gcggcacgcg agccggacag gacggccagc caggaaaagc
     7861 cgccgaggta gatcacgccg agggcggcaa gcatgccggc aacggcgcgg agcgtggtcg
     7921 agccccaacc tctttcggac acccagccag ccacccacgc ggcgcacgga taggagagaa
     7981 ggtagccggc cgtcggacct gcgagccaca tcggcccggc gtgaccaagc gcgaagacgg
     8041 ggagtccact gaagccctcg aggagataga gcgctgccgc ggcggcgccg cgaacggagc
     8101 ccaacgttac gccgacgagg agcacagcca gcggctgcag agtcagaggc actggcgaaa
     8161 acggcagcgg aatggcgacc tgcgcggcca gggcgatgac gacggtcgcg ccgatgacga
     8221 gcagcacgtc gagtgcggca cgcccgcgca cgaggcgcgt gagaagggcc gaagggatgg
     8281 tagcggcttt cgtttgcata gtttctccaa agcagagatc agagactgga gacaagagat
     8341 cagaaggaaa cgttgcctga tctctgatct ctagtcgctg atctctgtct cttttcagat
     8401 cagagatcag aagaaaacgt cgtcttgatc tctgatcgct tgtctctgat ctctgccttc
     8461 ttcattacga ggatcgccgg cagcagcgtg atcgccgcga ggccgctcag tccaattccg
     8521 aaggtggaag catacccaat cgatcgcagc cccggatagt gcgagaacgc gaacgagccg
     8581 taacccacga tcgccgtgag cgcggccatg acgatcgctt tgccggtctc atgcgcgctg
     8641 ccgcggaacg ctgcgacatc ttcccgataa cgctgcagca tgtagatcgc ataatcggta
     8701 gccacgccga tgatcatcag tcccacgaag atgttcatga agttgaactg aaggtcgagc
     8761 agcgccatca atccgagcat ccccacggct ccggcgacga acggcacgaa cgagagcgcg
     8821 gtcagcttca cagttcggaa actgaggaac atcagcacga agacggcgat gaagccgatg
     8881 atcgtcgagc gtgtggcatc cgccttcacg atgcgccgga gcgttgcgct gacgagattc
     8941 acacccgtga ggatgtcgcc gggatggcga tccggtaccg cgagcagctt cggcgaaaca
     9001 tcgcggcccc atttgccgcc cgtcggataa acattgatca cagacatcca gccgccgttg
     9061 accggtttga tgaaacgggt ggtcagcttc tcgatgtctt tgttgccgat ggtctggatc
     9121 gtgaccggct gcgccggttg cagcgtctgc gcgaagagct tcatgtaatc gtcgtaagcc
     9181 tcgggacgga agccgttctc agcgagcgcg gcgcggaacg tgtgatcgac tcgcgcgaag
     9241 ttgaactcat tgccgctgcc ggcgcgaagc cggtcgatga tcttctgctg atcgactgcc
     9301 ggcggcagga agctggagat cgattgatac gaggcgatgg tgtggtcgcg cacgaggccg
     9361 tcgaggtcct cggcagcggc gcgcgtcttc tccagcgctt cctcaaccgt ctttccttcg
     9421 cagacgtaca tcatgaaatc gaacgactgg ccgaactttt cggtgatcct cgtctgattg
     9481 aggaccccgg tgttcccctt cgcgcggaga tcctggatgt tgtcgctgaa cttcacgcgc
     9541 gtcgccagca ccccgcaggc cacgacgaag accacccaga ttccgatcgt gattcccggc
     9601 cgggctacgg agatgtcgat gagtttgccg gcgccgaacg agtgcagata gagcttcggc
     9661 gcgcggcgag cctgcttccg ctccgaaaac acgatgagcg cgggcagcag gaacatcacg
     9721 cacaacagaa agaacacgat accgacgccg gtcagcaaac caagctgcgt catgccgcga
     9781 aagtcggtcg ccagaaacgc gaagaacgtt ccagccgtgg tgatggcggc aacgaaaacg
     9841 ccgggcatcg tcgagcgcat gatcacgcgg atggcgtcct gcatcgacgc tccgcgattg
     9901 cgctcgtcga cgtagcgtcc gtagagcacg gtgatgaaat cgatccccag tcctgcgaga
     9961 agtgcggcga atcccgcgct cgcctgcgag agcttgccgt aggtgacgcc agccattccg
    10021 aacgtcagca ggaggccaag cgcgagcgga atgccggcgt agatgacgga ggccagccgg
    10081 cgaaaggcgt agagaaacag cgccagaaca ccgaccaccg atccgatgat gttgatgatg
    10141 acgtcctgac ggatcaggtc cgcgtcgccg accgcgatct cgtacccgcc tgtgtgcgaa
    10201 accttcggca ggggcgttcc cggctcggca gtcttctgga actccttcag cgcctgcgcc
    10261 tcgagcagcg tcccttcgct cagcaactct ttgccgaacg gcacgtcctg cgccggccgg
    10321 cgcggcttcg tgaggatcag cagcatgctg tgatccgacg acatgtagta gccgctcgag
    10381 gtgtcgatct tgaatccgct gccggcggac tgaaacttgc gaaggaagac cgggacgagg
    10441 ttgaagggat cgtattgaac gagcggcttc atcgcgaggg cttgcggcgt ctggagcatg
    10501 gcgtggttgc gcgccaccga ggcgcggatg ccttcgtccg tcagtttcgc agccacttcg
    10561 tcgagctcag ccggcgacag gaacagcagc gccttgggca gaatgatctc gatgaagtcg
    10621 agcggattgg ggatcttgta cgtcacatcc tcgatcttcg gattcttccg gtacccctcg
    10681 gcaatggcat cgatgagcga gtcgtattcg tgaacgtcac gccctttcgg catgtcgatg
    10741 acgacgatgt gatagtcgat cgtgccgagg tccttcagca ctttgcggaa ctcgttgacc
    10801 tgccggttct gctgcgggat gaggttgagc aggtcggggt cgaacgacag gcgcgtggcg
    10861 ccggccacgg caagaacggc gagcacgagc ccggcgatga ggatttcttt gctgcgccgc
    10921 aggcagagtc gcgcgagcgc gtcgaggatt cggtcagtca attaaggtct cgcggtatcg
    10981 ttcgtggcgc gcggaaagcg gggggataac aacatgcgca gtcagatcag tcaagttgca
    11041 ttcctgatgg tgttgctggt tgcggcctgc cagaaggccg agccactcac tgctgcaaaa
    11101 gccgagcaga tcctggcgag ctcccgtttt gcggccgagc ccatctacgc tgaggtgccg
    11161 caaaaggtgt ggtggaacgc gcactttccg aaggatgagt tcgacgagcg gtcgcttcgc
    11221 acgttcgaca acctcacgaa agccggcctc atcaccgtca ccggtggccc aacccccgac
    11281 ggcgccgcgt acgtcgcgaa gaccacgcag aagggctttc ccgtcctcgg caccgcaccg
    11341 agcgcccgcg gtccggtctt tcgcggcctg atctgctaca aggtctacga cggtctgcga
    11401 aactttcagc gccatccgac cgagccgacg accggacacg gtgagctggt gtggcactac
    11461 accaagccga cgacgctcta tccgatgttt gagacgaagc agaacaagcc gctcgacaag
    11521 cccttccttt cgctggtgtc gttctactac aaggatcacc agtggcagtt cgacgtcacg
    11581 gtgagaaaga cggaggcgga gtaaccgacc cagcctccac ggccctccga ctttaccgca
    11641 acgcctttga cgatcttctc gggcacgagc tcgaggctgg atgtgggtgg cgcgaccgga
    11701 gcggctcacg ttgttacagt gcggtgagtc ttacagcgaa gaccgcggac agcttacgga
    11761 gtggcagcag tttcttgaat gccgcgaacg cctgaccgag gttcgttcct gaaaacagca
    11821 tgtgaacctt cacaccgcga gctgcgtgcg cgcgaagtgt cgagcatgtg tcagctctat
    11881 ctctgttagc ggcaatgaca ccgttccgtg tccccgtcac gattatcgcg acctatatag
    11941 atacacaggt ccaaccaagc tgcataggcg tgttgaccat ggagacttcg ctatgagaac
    12001 tgtgagaacg tgtgtcgcga cactagccct tttgttcatc ccgttgttat taaccgccgg
    12061 cgaaggacgg atccggcgaa gccctaatgc tgtcaagggc gagtacatcg tcgtattgaa
    12121 cgattccgtt tcacgcgaaa acatttcctc cgtcgcccat caaatggccg cccagcacgg
    12181 cggctccatt caacgcatct ggcaggacgc tttgaagggc tatttcgccg tcatgaccga
    12241 agcgcaagcc caagccctca gccacaatcc caacgtcaaa tacgtcgaag aaaacgccgc
    12301 aatgttcacg tccagcagag ttccgacgaa cgttgatccg gcgtgcgatt acccctcgaa
    12361 ttgcacgacg gcggacaacc gcctctggca tctcgacgtt ctggatcaga acgacgcggt
    12421 aggcactcat gactacgcct attgcacgga cggcagtggc gtctatgtgt acgtcgtcga
    12481 tttcggtgtg atgcgcgccc atagggaatt caacaatgat cctaaccgcg ttgccgatgg
    12541 ttataacgcc agcggcgatt gtctcaacaa agacagccat ggccattgcc tcgcctctct
    12601 ggattactat ccggcatacg acccatgtca cggatcgccg acagccgcgg atcaggacat
    12661 gggcatggaa gtctttggca acagccacgg aacaggagtg gggtcgctcg tagccggacg
    12721 gaatatcggc gtggcgaaga acgccacgat cgtaccggtc aaggtggcgc gctgcggtga
    12781 attctcctca aagctcatcc agcgcaacac cacctaccca cccggggtga tcgtgcgctc
    12841 gaaccagggt ttttaccaaa cattgaacac cggcaccacc ggtccgggcg tgccgaaccc
    12901 ctgctgctct ccggacggaa gcttgaacct gcagttcctc ggcaacgacg gtctcactaa
    12961 tttcgggacc gtgcagatga tcattgaggg actggactgg atcctccggc ccgtagaaca
    13021 aggcgggaat ccctatccga agtcaccggc cgtggtgacg ctcagcacct acagggtggt
    13081 tggtaccgat ggcgttacga acacgccact tggctcctca ctgagtttcg aggacgctat
    13141 cgccaatctg ttgaagtaca acaacggcca gggcatcacc gtcatcgcct cggcgaacaa
    13201 ccaggacgcc aacgcctgcg acaccagtcc cggcaggatg tcgcgcaaca acccgaacaa
    13261 tccgaacgat ccgaaccacc cctacaaagt catcaccgcg ggcgggacga tgctccggaa
    13321 caatcccgat tccaatcctg ccaccggcgg ctccaacgtg aacctgccgg aacccaccta
    13381 cgactcgagc aaggcaacgc gcattgcccg ctggcgctgc aacgccggag actccgacgg
    13441 atgctccgcg aacatctacg cgcaacctcc acccaccgct ccgaacccag ccaccaattc
    13501 gagcgcgtat gtgggctgga ccctcggctc gaatggcgga cagtgcgtaa ccctctttgc
    13561 tccggccaag aacatccccg tggcgacgat tgccgccgat gccggtggca atccctccta
    13621 tcgcgattcc agagccacca acggtctggc ctccggaact tcctggtctg cgccgttcgt
    13681 cgccggcgta gtcgctcgca ttcttcagaa caatccggga tacagcgtcg atcaggtcta
    13741 ctcggctctg atgagcaaca ccaccccaga tctcgacccc atcgagctcg acccgcccgg
    13801 cgtcaccgga acgccgaacg cgcttctgca catccccgac gtcagcatcg ctccattgcc
    13861 ggccaccatc accggtccct tcacggcatc cgcttcggga accgctccgc tgacgtatca
    13921 gtgg
//
LOCUS       DATLVW010000072         4183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_457752, whole genome shotgun sequence.
ACCESSION   DATLVW010000072 DATLVW010000000
VERSION     DATLVW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457752"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..861
                     /locus_tag="VNN25_02450"
     CDS             313..861
                     /locus_tag="VNN25_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90414.1"
                     /translation="MSDNVNNTRGTSNPDPANLTAEAVIAQLRTMRSQIANVAPLSKE
                     QRKLVQRRLRVLPPPVVEASISVIGSSDTVAQAVGQPLGDVLQLQSDSVHWNLVADEL
                     RSFLKGIEGANLLRRDQLAFIASQAYLFGSQLARSPVNADLVPQVEEIKRLKTLARRK
                     KTTQNPPPSPAHETSTTPKNEE"
     gene            1167..3173
                     /locus_tag="VNN25_02455"
     CDS             1167..3173
                     /locus_tag="VNN25_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HXH90415.1"
                     /translation="MNALSLRVFSFIASLSIAACATLAAQTAKSPAPPQPVLMPPSIA
                     APVDQPYAGPVQLEVDLTNVTDRVAHVREQIPVQRNARELVLLYPQWIPGAHSPVGPI
                     SRIGGIVVTVDGRRVQWIRDQINVYAFHIPLGTTARTVELTFDYLSPIKPAEGRIEWS
                     DAIVDLAWNEVAMYPAGFFSRDIPFDVTLKLPQGWKHATALETASEEGSVVKFKRTTL
                     NTLVDSPLYAGVHFKRVDLSPSPTDVVRLNVFADDEKDLEISKDALEKHKNLAIEADK
                     LYQSHHYDHYDFLLLLSSNVGGIGLEHHQSSEDGSRPNYFTEWDRGNLRSDLLAHEYT
                     HSWNGKFRRPAGLWTPNFNVPMRDDLLWVYEGMTQYWGSVLAARSGIRTPEQARYVFA
                     RVAAGFEVSPGRDWRPLIDTTNQPTVSERRPMSWGSWQLGENYYTEGALIWLDADTKI
                     RELTKDRKSLDDFAAAFLGDFNGSFVTVTYDLQDIVKILNTIAPYDWMKFFRDRVDAL
                     HPAVPIDGITNGGYKLVYSDKIPSWLTRTETAAGGADFSTSLGFSVGGQRPGTAAAGP
                     ESMDLGNVWWNSPAFKAGVTPDMHIVSVDGKAYTSKVLREAIIEAEQTRRPIELQFRR
                     GEDYKSFSIPYYGGLRIPSLERVDGTPDRLDEILAPSKRPLPAM"
     gene            3461..4066
                     /locus_tag="VNN25_02460"
     CDS             3461..4066
                     /locus_tag="VNN25_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH90416.1"
                     /translation="MSTAAALREHDDTSNADHIIVIHDVTWEDYERLLDIRGDHSAPR
                     ISYLNGEVEIKSPSKDHERIKSYIGHLLETWCIDRGIEVRPFGSWTLTREENERAAEA
                     DECYTFGTEPRESPQLAIEVEWTRGGIDKLEIYRKLGVDEVWYWRKGVIEIYALAGGT
                     LTRTQRSRLLPHLDVELLASMLDRGTLTQAVRDFRKALQES"
BASE COUNT          834 a         1324 c         1255 g          770 t
ORIGIN      
        1 ctccacgcgt tgccgcttga caagtcgtct caaccggact taacctttta ttacaaagtc
       61 gaagatcgta ggacctccga ttttggcggc tgtcggcggt acgggctgtg ctggatagca
      121 caaattggtc ctgtaccggc gtgggagttg agcttgctcc tcctgccgcg ggcacccgag
      181 gtgtgtacac acgtcgggaa aacatcaacc ggaagaatcg gcgtagccgg gacttccagc
      241 cggtggccgc tcgcacttgt cgacgagtcg tgacgtcacc tccggcctta gctcgcgtaa
      301 ggaaggtatg tcatgagtga taacgtcaat aacacccgtg gtacatcgaa ccctgatccg
      361 gcaaacctga ccgccgaagc ggtcatcgcg cagttgcgca ccatgcgctc gcagatcgct
      421 aacgtcgcgc cgttgtcgaa ggaacagcgc aagctggtcc agcggcgact gcgggtgctg
      481 ccgcccccgg tcgtcgaagc atcgatcagc gtgatcggct cgtcggacac ggtggcgcag
      541 gctgtgggcc agccgctcgg cgacgttctc cagcttcaga gcgactctgt ccactggaac
      601 ctcgtcgccg atgagctgcg ctctttcctc aaaggcatcg aaggggcgaa tctgctccgt
      661 cgcgaccagc tcgcgttcat cgcatcgcag gcgtatttgt tcggatcgca gctcgcgcgg
      721 agcccggtca acgccgatct cgtgccgcag gtggaagaga tcaagcgtct gaagactctc
      781 gcgcgccgca agaagacgac gcagaaccct ccgccgtctc ccgcacacga aacgtccacg
      841 acaccgaaaa acgaagagta ggggaggttg cgaacccggg cgcgctcgca ggcgcgctcg
      901 ggtccatgtg cgtttagcgg aaggactttc tcacgcgaag acgcgaagac gcgaagggtt
      961 tgacgttgcg acgctctcct tcgcggtctt cgcgccttcg cgtgagaaat gagttgcggg
     1021 caagccagcg ctgagttctc ggcgcctgtg gcgaacgttt tccggtaagt tgcccgaaca
     1081 cgatccgcga catcaaggcg acgccgtcaa tggcataaga agtgggtcga tcccatcgct
     1141 cgattcgacg tagactctcg cgcgccatga atgcgctctc cctacgcgtc ttctctttca
     1201 tcgcatcgct gtcgatcgcc gcctgcgcca cgctggccgc gcaaacggcg aagagccctg
     1261 cgccgccgca gccggtgctc atgccgccgt cgatcgctgc gccggtggat cagccctacg
     1321 ccggccccgt gcagctcgag gtcgatctga cgaatgtcac ggaccgcgtg gcgcacgtgc
     1381 gcgagcagat tcccgtccag cgtaacgccc gcgagctggt gctgctgtat ccgcaatgga
     1441 tccccggcgc ccactcacct gtcggtccga tctcgcgcat cggcggaatt gtcgtcaccg
     1501 tcgatggaag gcgcgtgcag tggatccgcg accagatcaa tgtgtacgcc ttccacattc
     1561 cgctgggaac gacggccaga acagtggagc tcacgttcga ctatctctcg cccatcaagc
     1621 cggcggaagg ccgcatcgaa tggtccgacg ccatcgtcga tcttgcgtgg aacgaagtgg
     1681 ccatgtatcc cgcgggcttc ttctcccgcg acattccgtt cgacgtcacc ctcaaactcc
     1741 cgcagggctg gaaacatgcg accgcgctgg agacggcatc cgaggaaggg tccgtggtga
     1801 agttcaaacg gacgacgctg aacacgctcg tggattcgcc gctttatgcc ggagtgcatt
     1861 tcaaacgcgt cgacctttcg ccttcaccaa ccgacgtcgt tcgactgaac gtcttcgctg
     1921 acgatgaaaa agatctcgaa atctccaagg acgcactcga aaagcacaag aacctggcca
     1981 tcgaggccga caagctgtat cagtcgcatc attacgacca ttacgatttc ctgctgctgc
     2041 tcagcagcaa tgtcggtggc atcggactgg agcaccatca gtcgagcgaa gacggctcgc
     2101 ggccgaacta cttcaccgag tgggatcgcg gcaacctgcg ctccgacctg ctggcacacg
     2161 agtacacgca ttcgtggaac ggcaagttcc gccgtccggc cggcctctgg actccgaact
     2221 tcaacgtgcc catgcgcgac gacctgttat gggtttatga ggggatgaca cagtactggg
     2281 gaagcgtgct tgcggcgcgt tcggggatcc ggacgccgga gcaggcgcgc tatgtctttg
     2341 cgcgcgttgc tgccggattc gaagtcagcc caggccggga ttggcgtccg ctgatcgaca
     2401 ccacgaacca gcccaccgtt tcggagcgcc gtcccatgag ctggggcagt tggcaactgg
     2461 gggaaaacta ttacaccgaa ggggcgttga tctggctgga cgccgatacg aagattcgcg
     2521 agctcaccaa ggaccggaaa tcgctcgacg acttcgccgc cgcattcctc ggcgatttca
     2581 acgggagttt cgtcacggtg acttatgacc ttcaggacat cgtcaaaatc ctgaacacga
     2641 ttgcgccgta cgattggatg aaattcttcc gcgaccgtgt ggatgcgctg cacccggccg
     2701 tccccatcga cggcatcacc aacggcggct acaaactcgt ttacagcgac aagatccctt
     2761 cctggctgac cagaacggaa accgccgcgg gaggagccga tttctcgacg tcgctcggtt
     2821 tttcggtcgg cggtcagcgg cctggtaccg cggcagcagg tcctgagtcg atggatctcg
     2881 gcaacgtgtg gtggaacagt ccggcgttca aggcaggcgt gacgcccgac atgcacatcg
     2941 tctccgtcga cggaaaggcc tacacttcga aagtactgcg cgaagccatc atcgaggccg
     3001 aacagacccg gcgcccgatc gagctccagt tccgccgcgg agaagactac aagtcgtttt
     3061 ccatcccgta ttacggcggc ctgcgcatcc cttcgctgga gcgcgtcgac ggcacgcccg
     3121 accgcctcga cgagatcctc gcgccaagca aacgaccgct tccggcaatg tagcgccggc
     3181 tgcagcttct atctgcgtcc catctgcgtc atctgcggat aaggcttttc ccccgcctac
     3241 acgcccgagc tcgtcaccgc ggacgacggt tcggtaacga tttacgtcca ggcacacaaa
     3301 ccggagaagc acgccgccaa ctggctgccc gtcccgaaag gacacttcag ccttctgttc
     3361 cgcgtctacg ggccgctggg cagcgcgctc gccgggacgt acgttccgcc caagatccat
     3421 cggggcgcgc cgcggtgatg gcgggcggta cactttcatc atgtccacgg ctgcggcgct
     3481 tcgcgaacac gacgacacgt cgaacgccga tcacatcatc gtcattcacg atgtcacgtg
     3541 ggaagactac gagcgcctgc tcgacattcg tggagatcac tcggccccgc gcatcagcta
     3601 tctgaatggg gaagtcgaga tcaagagccc gtcgaaggat catgagcgga tcaagtcgta
     3661 catcggacac cttctcgaga cgtggtgcat cgatcgcggc atcgaagtga ggcctttcgg
     3721 cagttggacg ctgacgagag aagagaacga acgcgccgcc gaagccgatg agtgctatac
     3781 ctttggaacg gagccgcggg aaagccccca actcgcgatc gaagtcgagt ggacacgggg
     3841 cggcatcgac aagctggaga tttaccgcaa actcggcgtc gatgaagtct ggtactggcg
     3901 caaaggagtc attgaaatct acgctctcgc gggaggaacg cttacccgaa cgcagcgcag
     3961 ccggctcctt ccacacctcg acgtcgaact gctcgcttcg atgctcgatc gcggcacgct
     4021 cactcaggcc gtacgcgatt tcagaaaggc gctccaagaa tcgtaggcta ctcataggcg
     4081 ctggccaata agctgcgcgt ggatccaccc gcctgggtgt gccaagactt ctgcgcgaga
     4141 ggccgcgtgc gcgaggggcc acgttgtcga cgcgcgcggg ggt
//
LOCUS       DATLVW010000073         5532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_457754, whole genome shotgun sequence.
ACCESSION   DATLVW010000073 DATLVW010000000
VERSION     DATLVW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457754"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..873
                     /locus_tag="VNN25_02465"
     CDS             313..873
                     /locus_tag="VNN25_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90417.1"
                     /translation="MSDPVNNTPGTSNPDPAMLTAEAVIGQLRSMRSQIANVAPLSKE
                     QRKLVQRRLRVLPPPVVEASISVIGSSDTVAQAVGQPLGDVLQLQSDSVHWNLVADEL
                     RSFLKGIEGANLLRRDQLAFIASQAYLFGSQLARNPVNADLVPQVEEIKRLKTLARRK
                     KATQNPPPPFPAPAPHETSAAPKNAE"
     gene            983..2476
                     /locus_tag="VNN25_02470"
     CDS             983..2476
                     /locus_tag="VNN25_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90418.1"
                     /translation="MQFEHDLLISYAHIDDQALVEGENGWVARFHRLLEIRVGQLLGQ
                     TPKIWRDLKLQGNDYFADTILDRLPRIAALVSIFSPRYVQSEWCTRELREFCLAAERS
                     GGVRLAGKARVFKVVKTPISLDRFPEQVQPMLGYDFFVYDDAGRPRELAQVHAQDSDR
                     VFLAKVDDLAYDIVQLLEMLRRDEIVVTQEDRKGTVFLAEVSFDLRDDREAIKRDLIH
                     KGYEVLPDRPLPLIASELDGVVREQLLRSKLSIHLIGRNYGLVPDGAAQSVVERQQEL
                     ASQLAAIAGFSSVIWISPGVEVEDERQRQFIGRLHTDPAVHAAAELLEVPFEDLKTLV
                     YRTLAPPPAPVLTPPAVPVSSAGELTRIYLLCDQEDVEATRPIEDFLFDQGFEVILPI
                     FDEDEAQARLEHEEHLKSADAILIFYGASGERWLQRKLGDLQKCKALGREKPWLGSAV
                     YLGAPATPHKERFRTHAATVIHEPAGGFTPAALEPFLSEVSRARGTQ"
     gene            2473..5496
                     /locus_tag="VNN25_02475"
     CDS             2473..5496
                     /locus_tag="VNN25_02475"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90419.1"
                     /translation="MTSALESYAEEWSNPFPGLRPFEPDEDYLFFGRERQTDDLLKRL
                     RTTRFLSILGTSGSGKSSLVRSGLIPSLYGGGMTVAGSRWRMAIMRPGDDPIGNLAAA
                     LSATEALGAQSDEEGMTRSFFETTLRASNLGLIECVQQARIAPRDNVLVLIDQFEELF
                     RYKRSRRAAGRDDALAFVKLLLATRGSEAPLYIALTMRSDFIGDCMEFGGLPEVINDG
                     VYLVSRMTRDELRSAITGPVAVGGATIAPRLVSRLLNDVGDDPDQLPILQHALMRTWE
                     RWRAEHEPGEPLDLRHYEAIGTMQTALSRHAEEAFAELSGNEQVIAEKMFKALTDKET
                     DVRGVRRPAAMAEICALTGASLGQVAAVIDHFREHGRTFLMPPAGTALDDDSIIDISH
                     ESLMRVWTRLSQWADDESKSAQTYMRVARAAELHEQGAAALWRDPELQLALTWRDAAK
                     PVAAWAARYDASFDRAMAFLDASKAERDREIREKETRRRVLAAILIAASTIIAAFGVY
                     AFKQKMQAEAAQKVAVYARTRAEEALARVKQERARADTEKKRAEDQAFVAEQQKQEAD
                     KERKNADVERATALEQKTLAEKQTAVAQQQTTIATGERVKATQAQQVATKNAQEALDQ
                     KRKADDARIEAERLSHREEAHALALKVIAQTARSDSSAHDALAAERLNRENGGRIEDP
                     DIFKAMLLTLQDNRLHLWQGSDSFRSLVIAPDGRTAFAGGDDGSLFTFDLTRDDVPRK
                     IGKFPGGVRALAARGNLLAIGTANAHISLRDLHDPAKTIRDLEAGTDVISALAFQPNG
                     PLLAAGSFDGSLRVWNTSDTSPPQKKASPAGESARINAVAFDAGGTSLAAGTSQGAFV
                     VRTADLTGGEDRVCEGDVRSLAIGAGKLACGLSDGKILEVGLHGSKTPQRLEGHTSSV
                     NSLRYNARGDVLLSGSSDGTVRLWDRQAGNVPPIVLSGHESWVWAAVFASNEDRVLSA
                     GKDVRLWPAKTKVLAADLCAVIGGGDKELCEMVKH"
BASE COUNT         1033 a         1760 c         1771 g          968 t
ORIGIN      
        1 ctccacgcgt tgccgcttga caagtcgtct caaccggact taacctttta ttacaaagtc
       61 gaagatcgta ggacctccga ttttggcggc tgtcggcggt acgggctgtg ctggatagca
      121 caaattggtc ctgtaccggc gcgggagttg agcttgctcc tcctgccgcg ggcacccgag
      181 gtgtgtacac gcctcgggaa aacatcaacc ggaagaatcg gcgtagccgg gacttccagc
      241 cggtggccgc tcgcacttgt cgacgagtcg tgacgtcacc tccggcctta gctcgcgtaa
      301 ggaaggtatg tcatgagtga tcccgtcaat aacacccctg gtacatcaaa ccctgatccg
      361 gccatgctga ccgccgaagc ggtcatcggg caattgcgca gcatgcgctc gcagatcgcg
      421 aacgtcgcgc cattgtcgaa ggaacagcgc aagctggtcc agcggcgtct gcgggtgctg
      481 ccgcccccgg tcgtcgaagc atcgatcagc gtgatcggct cgtcggacac ggtggcgcag
      541 gcggtaggcc agccgctcgg cgacgttctc cagcttcaga gcgactcagt ccactggaac
      601 ctcgtcgctg atgagctgcg ctcgtttctc aaaggcatcg aaggggcgaa tctgcttcgt
      661 cgcgaccagc tcgcgttcat cgcatcgcag gcgtacttgt tcggatcgca gctcgcgcgg
      721 aacccggtca acgccgacct ggtgccgcag gtggaagaga tcaagcgtct caagactctc
      781 gcgcgccgca agaaggcgac gcagaatcct ccgccgccgt ttccggcgcc tgcaccccac
      841 gaaacgtccg cggcaccgaa aaacgcagag taaagaacgt tgtgagcccg ggcgtgcttg
      901 caggcgcgcc cgggctccac ggaagtcgga atcccggatc ggcgggtatc gcgaacagtg
      961 ttactgttta cataagcaat caatgcaatt cgagcacgat cttctgatca gttacgccca
     1021 tatcgatgat caggcgcttg tggaaggtga aaacgggtgg gtggcgcgct tccatcgtct
     1081 gctcgagatt cgtgtcggcc agctccttgg tcagacgccg aagatctggc gtgatctgaa
     1141 actgcagggc aacgactact tcgccgacac catcctcgat cgtctgcctc gcatcgcagc
     1201 actcgtctcc atcttctcgc cgcgctacgt ccagtcggag tggtgtactc gtgagctgcg
     1261 agagttctgc ctcgccgccg aacgctccgg cggcgtgcgt ctggctggaa aggcgcgtgt
     1321 cttcaaggtc gtcaagacgc cgatcagtct cgatcgcttt cccgaacagg tgcagccgat
     1381 gctcggctac gatttctttg tctacgacga cgcgggccgg ccaagggagc tggcgcaagt
     1441 gcacgctcag gattcggatc gcgtcttcct cgccaaagtc gacgatcttg cctacgacat
     1501 cgttcagctt ctggagatgc ttcgccgcga tgagatcgtc gtcacgcagg aggatcgaaa
     1561 agggacggtc ttcctcgcgg aagtcagctt cgaccttcgc gacgaccgcg aagccatcaa
     1621 acgcgatctg atccacaagg gctacgaagt gcttcccgat cgtccgctgc cgctcatcgc
     1681 ctccgagctc gatggcgtcg tccgcgagca gcttctgcgc agcaaactct cgattcatct
     1741 catcggccgc aactacggcc tggttcccga tggtgcggcc cagtcggtcg tcgagcgcca
     1801 gcaagagctc gcatcccaac tcgcggcgat tgccggcttt tccagcgtca tctggatctc
     1861 tccaggcgtc gaggtcgaag acgagcgtca acgccagttc atcggccgtc tgcacaccga
     1921 tcccgcggtc cacgccgctg ccgagttgct ggaagtgccg ttcgaagatc tgaagacgct
     1981 cgtctaccgc acgctcgcgc cgccgcccgc gccggtactg actccgccag cggtgcccgt
     2041 ctcctcagcc ggcgagctca cgcgcatcta ccttctttgc gatcaggagg acgtcgaagc
     2101 gacccggcca atcgaggact ttttgttcga ccaggggttc gaagtcatcc tccccatctt
     2161 cgacgaagac gaagcgcagg cgcggctgga gcacgaggag catctgaaat cggcggatgc
     2221 aattctcatc ttctatggcg cgagcgggga gcgatggctg caacgcaagc tcggcgatct
     2281 gcagaaatgc aaggcgctcg ggcgcgagaa gccgtggtta gggtcagccg tctacctcgg
     2341 cgcgccggcc acgccgcata aggaacgatt ccgtacgcac gcggctaccg tcatccatga
     2401 gccggccggc ggcttcactc ccgccgcact cgaaccgttt ctcagcgaag tctccagggc
     2461 ccgagggacg caatgacctc ggcgctcgag tcgtacgcgg aggaatggtc caatccgttc
     2521 cccggtctgc ggccattcga gccggatgag gactatctct tcttcggccg cgagcgtcag
     2581 accgacgacc tgctgaagcg gcttcgtacc acgcgcttcc tctccattct cggcacctcg
     2641 ggaagcggca agtcatcgct ggttcgctcg ggactcattc cgtcgcttta cggcggcggc
     2701 atgaccgtcg ccggatcgcg ctggcgcatg gccatcatgc gtcccggcga cgatccgatc
     2761 ggcaatcttg cggccgcgct cagcgcaaca gaggccctcg gtgcgcagtc ggatgaggag
     2821 gggatgacgc gttccttctt cgagacgacg ctccgcgcca gcaacctcgg gttgatcgag
     2881 tgcgtgcagc aggcgcgcat cgcgccgcgc gataacgtgc tcgtgctcat cgatcagttc
     2941 gaggagctct tccgctacaa acgcagccgc cgcgcagccg gacgcgacga cgcgctcgcg
     3001 ttcgtgaagc ttctgctggc cacgcggggg agcgaggcgc cgctctacat cgcgctgacc
     3061 atgcgctccg acttcatcgg cgactgcatg gagttcggtg ggctgcccga ggtgatcaac
     3121 gacggtgtgt atctcgtttc gcggatgacg cgcgacgaat tgcgttcggc catcaccggg
     3181 ccggtcgcgg tcggcggcgc cacgatcgcg ccgcgtttgg tatcgcggct gctcaacgac
     3241 gtcggcgacg atcccgatca gctccccatc ctgcaacacg cgctcatgcg gacgtgggaa
     3301 cggtggcgcg cggagcacga gccgggcgag ccgctcgacc ttcgccacta cgaagcgatc
     3361 ggcacgatgc agactgcgct ctcgcgccat gccgaggagg ccttcgcgga gttgagcggt
     3421 aacgagcaag tcatcgccga gaaaatgttc aaggcgctga cggataagga gacagatgtg
     3481 cgcggcgtgc gccggcccgc ggcgatggct gagatctgcg cgctcaccgg tgcgtcgctc
     3541 ggacaggtcg cggcggtgat cgatcacttc cgcgagcacg gccggacatt cctgatgccg
     3601 cctgccggca cggcgctcga cgacgactcg atcatcgaca tctcccacga gagtctgatg
     3661 cgcgtctgga cgcgtctctc gcagtgggcg gacgacgagt cgaagtcggc ccagacctac
     3721 atgcgcgttg cccgcgccgc cgagctccac gaacagggtg cagctgcgct gtggcgcgat
     3781 ccggaactgc aactcgcact aacgtggcgc gatgccgcga agccggttgc ggcgtgggct
     3841 gcgagatatg acgcgtcgtt cgatcgcgcg atggcgtttc tcgatgccag caaggccgaa
     3901 cgtgaccggg agatccgcga gaaagagacg cggcgccgcg tgcttgcggc gatcctgatc
     3961 gcggcgtcga cgatcatcgc cgcgtttggc gtgtacgcat tcaaacagaa gatgcaggcg
     4021 gaggcggcgc agaaagtggc ggtttacgcg cggaccagag cggaggaggc actcgcgcgg
     4081 gtaaagcagg agcgggcccg cgctgacacg gagaagaaac gcgcggagga ccaggcgttc
     4141 gtcgcggagc agcagaaaca ggaggcggac aaggagcgaa agaatgccga cgtggaacgc
     4201 gccacagcgc tggagcaaaa gacgctcgca gagaagcaga ccgctgttgc acagcagcag
     4261 acgacgatcg ctaccggcga gcgggtgaag gctactcagg cgcaacaggt tgcgacaaag
     4321 aacgcacagg aagcgctcga tcagaagagg aaggcggatg acgcccgcat cgaggcggag
     4381 aggctcagcc accgcgagga ggctcacgcg cttgcgctga aggtcattgc gcaaacggcc
     4441 cggtccgaca gctcggcgca tgacgcgctc gcggccgaac gtttgaatcg tgagaacggt
     4501 ggcaggatcg aagacccgga catcttcaaa gccatgctgc tgacgctgca ggacaaccgg
     4561 ctgcacttgt ggcaaggctc ggattccttc cggtccctgg tgatcgcacc cgacggccgg
     4621 accgccttcg ccggcggtga cgacggcagc ctgtttacat tcgatcttac tcgtgacgat
     4681 gtaccgcgga agatcggcaa gtttcccggc ggcgtgcgcg cgctggcggc gcgcggcaat
     4741 ctgctcgcga tcggcacggc caacgcccac atctcccttc gcgatcttca cgatcccgca
     4801 aagaccattc gcgacctcga agccggaacg gatgtgatca gcgcgctcgc gttccagccg
     4861 aacggcccgc tactcgccgc cggcagcttc gacggctccc tgcgtgtatg gaacaccagc
     4921 gacacgtcgc cgccgcagaa gaaggccagt cctgccggcg agagcgctcg aatcaacgcc
     4981 gtcgcgttcg acgccggcgg aacgtcgctc gcggccggaa ccagccaggg cgcttttgtc
     5041 gtccgaacgg ctgacttgac cggtggcgaa gatcgcgttt gcgagggtga cgtccgcagt
     5101 ctcgccatcg gcgctggaaa actcgcctgt ggcctcagcg acggaaagat cctcgaagtg
     5161 ggacttcacg gttcgaagac gccccagcgg ctcgaaggac atacgtcgtc cgtaaactcg
     5221 ctccgctaca acgcgcgcgg cgacgttctc ctctccggaa gctcggatgg cactgtgcgt
     5281 ttgtgggaca ggcaggccgg aaacgttccg ccgatcgtcc tgtcgggtca cgagtcatgg
     5341 gtgtgggccg cggtcttcgc ctcgaacgaa gacagggttc tctccgccgg caaggacgtg
     5401 cggctgtggc ccgcaaaaac gaaggtactg gccgcggacc tttgcgcagt catcggtggt
     5461 ggggacaaag agctatgcga aatggtgaag cactgatgcg aagctgggtc tctgccgctc
     5521 ttttccttct cg
//
LOCUS       DATLVW010000074        11114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_169786, whole genome shotgun sequence.
ACCESSION   DATLVW010000074 DATLVW010000000
VERSION     DATLVW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169786"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VNN25_02480"
     CDS             <1..242
                     /locus_tag="VNN25_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90420.1"
                     /translation="IACRIQMGINFLYPTPTFARGFARAIDFWGTADVYNVCDSPDTA
                     DAMAMWADWKDVGADLVEAVRILALDAPVKNFSRP"
     gene            262..525
                     /locus_tag="VNN25_02485"
     CDS             262..525
                     /locus_tag="VNN25_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90421.1"
                     /translation="MNLYLLFAIALGAISGTAEPTVARPRFADVAAECRAMSCEQSIA
                     SPRSTLHHARSESTAPRVVESAPARIEAPLSGAATPRAPATDC"
     gene            575..3319
                     /gene="secA"
                     /locus_tag="VNN25_02490"
     CDS             575..3319
                     /gene="secA"
                     /locus_tag="VNN25_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXH90422.1"
                     /translation="MINKILTTIFGSKHERDVKKMLPTVAQINALEPEISALSDDGLR
                     GKTDEFRARLRPAVEALETAKKEIPRDEVVVRTAKEDLQAALNEILPEAFAVAREAGK
                     RVLNMRHFDVQLMGGIVLHSGKISEMKTGEGKTLVATLPVYLNALTGRGVHVVTVNDY
                     LARRDSEWMGRLYKFLGLTVGVIQNHHGDRERQEAYGCDVTYGTNNEYGFDYLRDNMK
                     FDLESMVQRDHVYAIVDEVDSILIDEARTPLIISGPSEERPDKYYSVDRIIPRLVKET
                     DYQVDEKQHTSVLTEEGVLHAEKLLGVNNLYDPDNMDTLHAVQQALRAHTLYKLDVEY
                     VVKDGEVLIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIEAENQTLATITFQNLFRMY
                     EKLAGMTGTADTEAAEFDKIYKLEVVVIPPNRQMVRKDQADLVYRTEREKFNAIVEEI
                     KVLNEKGQPALVGTISIEKSEALASALKRKGVPHVVLNAKYHEKEAEIVAQAGRKGAV
                     TIATNMAGRGTDILLGGNPEFAAAQKNRDVADPAERKRVEEEIQEQWKLDHDEVVALG
                     GLYIVGTERHESRRIDNQLRGRSGRQGDPGASRFYLSLEDDLMRIFGGERLMNWMEKL
                     GMEEGVPIEHGMVSRSIERAQKQVEGRNFEARKHLLEYDDVMNKQRESVYTLRRSILE
                     GREGKDYILNAADEIVDYLIETHIPEDARETPNEAELNAELYDFFGIDLRAANIDLSS
                     TPRAAVKDAVIEVVNRRYEEKEASIGTEMMSLHLKYILLQVIDQLWKDHLLNVDHLKE
                     GIGLRGYGQRDPLIEYKKESYEIFQEMMELIQDRVVKYLWKTEVVVEREGERQDEVQR
                     VQRAMPPPKPKAQPMVFSGAGGVSGPAQVQRKEPKIGRNDPCWCGSGKKYKKCHGNAA
                     "
     gene            3605..4075
                     /locus_tag="VNN25_02495"
     CDS             3605..4075
                     /locus_tag="VNN25_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90423.1"
                     /translation="MFYTLEAVSFTRDAVIHARDDVCCTRDAAFCTPDGMFCTPDEMF
                     CTRDEMFCTRDEMFCAPDGMFCTRDEMFWAPDEMFCTRDEMFCAPDGMFCTPDGMFCT
                     RDEMFCAPGEMFCAPDEMFCTHDAAVCTHDAVVCTPDPVICTHDAAFWTHDATL"
     gene            4206..5486
                     /locus_tag="VNN25_02500"
     CDS             4206..5486
                     /locus_tag="VNN25_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HXH90424.1"
                     /translation="MHASRLALTLFFSSAALTVHLAAQPALTSWKLNTTGETGTYTLQ
                     NGTKGTLPANVQLVQYDATYVYVSASGIPSYDIGPFPGNPNVPANQHYIAAFARTPVV
                     PATKVAAQLGVNGLYVNGVAMFNADDGNSWRNASRTESPQGDGIWHRNGGVVEAVGFD
                     PCEGHPQQQGVYHHHEYSPCLGAQLGATTTSHSPLLGFALDSFPMYGPYGYSSPLDPT
                     TGVRRMVSGYRLRSITQRHALPDGTILTPAQYGPDVDSNFVLGRYLEDFEWLAGLGDL
                     DAHNGRFCVTPDYPAGTYAYFLTLDEHGQPAFPYTIGLTFCGTPNIGTHATVPLTGVT
                     TLPVLAITSFVPPSGGAGSSVVITGKGFTGVKAVYFNEVPSASFSIVGDTQITATVPS
                     GGAAGRIRVDRADAYARSTTDFQPSQPRRRAAGK"
     gene            complement(5673..7559)
                     /locus_tag="VNN25_02505"
     CDS             complement(5673..7559)
                     /locus_tag="VNN25_02505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH90425.1"
                     /translation="MKHASPIIRSRWFARIAAITFVLLFVGASLLAETTGSANELAGL
                     WKAKKRFGPDARGALVLQRSGNAWTADMAGRIVPVTVANGELTFKLPDNGGTFRGRRD
                     DQGVIRGHWLRPGTALYWKATPISASPVVLKPDGPNRWRGNVDPIEETFTFYLLLQPQ
                     PDGSFRAVLRNPEFDLGTQQGVERLTRDGNALKLMGKRGGKEQVVASGTYDAENAVIT
                     FAIPGRGGSYDFLRDGDDSGFYPRGKQPGRYIYRPPLARDDGWQVSTLDEANIDRPTI
                     EKFVQSILDTPMDSQDAPQVHGVLIARHGKLVLEEYFHEQDRDLLHNTRSASKSLTSL
                     LVGAAMHAGVPLKLSSPVYQVMNGGTFPPDLEPQKRAMTLEHLLTMSSGYFCDDTNDD
                     APGNEDKMWEQEAEPDFYRYTMKVPMATPPGENAVYCSASPNLALGMLGRAAHEDPTS
                     FFDRLIGGPMKIRRYEWALDPAGHPYGGGGMALLLRDFIKLGQLMLNGGTWEGRRIVS
                     QEFVARAGAPLYHLRNIYYGYLWWGEDYPYKNRTVHTYSARGAGGQTVTVVPDLDLIV
                     ATFAGNFSSRKGMFAASTDPIPRIILPAVREPGDDKNAPVIEREYVTPYGASKDGSRV
                     SKKP"
     gene            7730..8080
                     /locus_tag="VNN25_02510"
     CDS             7730..8080
                     /locus_tag="VNN25_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90426.1"
                     /translation="MADRDFVVENLDFDVENLDIAVENVDIAVENLDIAVENRHCDVE
                     ILDIAVRNLDIDVKTHDIDVENLDIAVRDLDIAVENLDIDVENLDLVVVNLHLVVVNL
                     HFDVDDRRPTGRFE"
     gene            complement(8098..9291)
                     /locus_tag="VNN25_02515"
     CDS             complement(8098..9291)
                     /locus_tag="VNN25_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH90427.1"
                     /translation="MNVVFISGNRAWGGSEELWSAAAAVLAADGHTVTVFKSGVDESE
                     PRIRRLRALSCRVRDLARFPLMPRRLFTLVRTLSSAAAYGHEAMRLLIGLALSRRPDL
                     VLVSQGGNHDGYVLAEVCRRLRLPYAIVSHKASELYWPADGQQKTIAAVYRAAVAWFF
                     VSEHSRRLTEEQLGFPLPGAAVVRNPFLVPWEPRTDWPDDGNEWRLACIGRLHPKEKG
                     QDLLLRVLARRHWRDRPLAVTFYGEGLQRASLERMAQNLGLTNVTFAGFVHDVPAIWS
                     GHHGLILPSRCEGLPIVLVEAMLSGRVAIVTDVGGNAEVVEDGASGYVAAAATEDALD
                     EAMERAWNERDRWREVGAAAAARIRKLIPPEPERCFADRLVGLACSDAASSTSSYEAA
                     KTPHS"
     gene            9291..10361
                     /locus_tag="VNN25_02520"
     CDS             9291..10361
                     /locus_tag="VNN25_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXH90428.1"
                     /translation="MRYSRFILRDRLGHIVNPIVDLTRADQWWEYKLVTILSAFYATA
                     LVLHVAVSSLWVSALSLLLAIVAAAIYASVINDVTDLAGDREAGKRNAMAGRSRSMVA
                     ALVALSAAAGSLFAWLWRDDTPLLWSYLATWLAFSLYSLPAFRLKERGAAGVLCDAAG
                     EQLFPALVAVFLASRGAHRAVGVAWMASVAVWALAYGLRGILWHQLTDEDNDRAAGFR
                     TFVCRHPRAAPAIGTFIVFPIELGALAAMLWQIDSAWPPAFLVLYALYAVLSARRWQT
                     SPVIVVPQPRFFIVLHQFYSDLFPVALLIAGSVRDRRVLIVLAVHLLLFPRRVIHAIC
                     RLRASTARTVVNASEPHHGGVG"
     gene            complement(10280..>11114)
                     /locus_tag="VNN25_02525"
     CDS             complement(10280..>11114)
                     /locus_tag="VNN25_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90429.1"
                     /translation="CVRDPVVFTTALAARALSITPSAARVRSRATDFLLREMEPDGLW
                     RYPSTEHAGHDHIPLDVDDTSIASAALAAAGQRFPDNRALLLANRERSGLFRTWIVRW
                     WPHPRKIYRFFRETTAEIRDVDAVINANVVIYLGDCKETRPAIAHMLAVLRANDEMRS
                     TKWYESRFTVWYFFSHALAGIAPEAGGMIVPRIEAATPRNALELALAASALQLWNRTP
                     DLQPLIDAQLPSGAWPSAGFYHAGLERISKKEFQPTPPWWGSEALTTVLAVEALSRQI
                     A"
BASE COUNT         2075 a         3575 c         3448 g         2016 t
ORIGIN      
        1 ggatagcctg ccgaatccaa atgggcatca actttcttta cccgactcca accttcgccc
       61 gcggctttgc ccgagcgatc gatttctggg gaacagctga cgtgtacaac gtctgcgact
      121 cgccggacac cgccgatgca atggcaatgt gggcagattg gaaagatgtc ggtgccgatc
      181 tcgtcgaagc ggtgcggatc ctggcgctcg acgcccccgt gaagaatttc tcgagacctt
      241 aatgctcttt agcccttttg catgaacctc tatctcctct tcgcaatcgc gcttggcgca
      301 atctccggga cggccgaacc gaccgtcgcg cggccgcgct ttgccgatgt ggctgccgag
      361 tgcagggcga tgagttgcga gcagtcgatt gcatcgccgc gaagcacgct ccatcacgcc
      421 cgttcggaat cgaccgcacc ccgcgtcgtc gaaagcgctc cggcccgcat cgaagcaccg
      481 ctttccggcg ccgcaacgcc gcgcgcccct gcaactgact gctgagccgt tttctctcgc
      541 tcgcggttcg tttgttgctg ggaaggaaag caccatgatc aacaagatac tcaccacgat
      601 tttcggctcg aagcatgagc gcgacgtcaa gaagatgctg ccaacggtgg cgcagatcaa
      661 cgctctcgag ccggagatca gcgctctgag cgatgacggg ttgcgcggca agacggatga
      721 gtttcgtgcg cgtctccgcc ccgccgtcga ggcgctcgaa acagcaaaga aagagattcc
      781 tcgcgatgaa gtcgtcgtcc gcacggccaa ggaagatctt caggccgcgc tcaacgagat
      841 tctccccgag gcgttcgcgg ttgcacggga agccggcaag cgcgttctca acatgcgcca
      901 cttcgacgtc cagctcatgg gcggcatcgt tctgcacagc ggcaagatct cggagatgaa
      961 gaccggcgaa ggtaaaacgc tggtggccac gctgccggtc tacctcaacg ccctgaccgg
     1021 ccgcggcgtg cacgtcgtca cggtcaacga ttacctggcc cgccgcgact ccgagtggat
     1081 gggccgcctc tacaagttcc tcggcctcac cgtcggcgtg atccagaacc accacggcga
     1141 ccgcgagcgg caggaagcgt acggctgcga cgtcacctac ggaacgaaca acgaatacgg
     1201 cttcgattac ctgcgcgaca acatgaagtt cgacctcgag tcgatggtgc agcgcgacca
     1261 cgtctacgcc atcgtcgacg aggttgactc catcctcatc gacgaggcgc gcacgccgct
     1321 catcatcagc ggcccttccg aggagcggcc cgacaagtac tactccgtcg accgcatcat
     1381 tccgcgactg gtcaaggaaa ccgactacca ggtcgatgag aagcagcaca cctctgtcct
     1441 gaccgaagag ggcgtcctcc acgccgaaaa gctcctcggc gtcaacaatc tctacgaccc
     1501 cgacaacatg gacacgctgc acgccgtgca acaggcgttg cgcgcccaca cgctctacaa
     1561 actcgacgtc gagtacgtcg tcaaagatgg tgaagtcctc atcgtcgacg agttcaccgg
     1621 acgcctgatg cccggccggc gctggagtga cggtcttcac caggcggtcg aagcgaaaga
     1681 gggcgtcaag atcgaagccg agaaccagac cctggctacg atcacgttcc agaacctctt
     1741 ccgcatgtac gagaagctcg ccggcatgac cggtactgcg gacaccgagg cggcggagtt
     1801 cgacaagatc tacaagctcg aggtcgtcgt catcccgccg aaccggcaga tggtgcgcaa
     1861 agatcaggcg gacctcgtct atcgaaccga gcgcgagaaa ttcaacgcca tcgtcgaaga
     1921 gatcaaggtg ctcaacgaga aagggcagcc tgcgctcgtc ggcacgatct cgatcgagaa
     1981 gtcggaagcg ctggcgtccg cactgaagcg aaaaggcgtc ccgcacgtcg ttctcaacgc
     2041 caagtaccac gagaaggaag cggagatcgt cgctcaggcc ggacgcaaag gggcagtcac
     2101 gatcgccacg aacatggctg gccgcggcac cgacatcctc ctcggcggca accccgagtt
     2161 cgctgccgcg cagaagaacc gagacgtggc cgatcctgcc gaacggaaac gagtcgagga
     2221 agagattcag gagcagtgga agctcgatca cgatgaggtc gtcgccctcg gcggtctcta
     2281 catcgtcggt accgaacgcc acgaatcacg ccgcatcgat aaccagctcc gaggccgttc
     2341 gggacgtcag ggcgatcccg gagcttcgcg cttctatctg tcgctcgaag acgatctgat
     2401 gcgcatcttc ggcggcgagc ggctcatgaa ctggatggag aagctgggga tggaagaggg
     2461 cgtccccatc gagcacggga tggtgtcccg ttcgatcgag cgggcgcaga aacaggtcga
     2521 aggccgcaac ttcgaggccc gcaaacactt gctcgagtac gacgacgtca tgaacaagca
     2581 gcgtgagtcg gtctacacgc tgcgccgctc gattctcgag ggccgcgaag ggaaggatta
     2641 catcctcaac gccgccgacg agatcgtcga ttacctgatc gagacccaca tccccgagga
     2701 tgcgcgcgag acgccgaacg aggcggagtt gaacgccgag ctgtacgact tcttcggcat
     2761 cgacctgcgc gcggcaaaca tcgatctttc gtcaacgccg cgcgccgcgg tgaaggacgc
     2821 cgtgatcgaa gtcgtcaatc ggcgctacga ggagaaggaa gcgtcgatcg gcacggagat
     2881 gatgagtctc cacctcaagt acatccttct gcaggtcatc gatcagctct ggaaggatca
     2941 ccttctgaac gtcgatcacc tcaaggaagg aatcggcctg cgcggctacg gccagcgcga
     3001 tccgctcatc gagtacaaga aagagtcgta cgagatcttc caggaaatga tggagctgat
     3061 ccaggaccgc gtcgtcaaat acctctggaa gaccgaggtg gtcgtggagc gggaagggga
     3121 acgccaggac gaagtgcagc gcgtccagcg agccatgccg ccgccgaagc cgaaagcgca
     3181 gccgatggtc ttctccggcg ccggcggagt ctccggcccc gcgcaggtgc agcgcaaaga
     3241 accaaagatc ggccgcaacg acccctgctg gtgcggcagc ggcaagaagt acaagaagtg
     3301 ccacggcaac gcggcgtagg gaagcagaga tcagagacaa gacgtacgga gtgcggtcgt
     3361 ctcgaccgcg ggcattcggg cgtcctcgcc cgagtgcagt cggggcggag gtcgaaattc
     3421 gtgtcgaaca cactcttggg cggcgccctt agtctccaac tgtgagaaat gacaagctaa
     3481 aacgggcggg agcaaaccgc cttctctcct ctccctctct ctccgggctc cttggctgct
     3541 gctgacgttg ttggcgtcgc gccgtccgcg agcgggctgg gaacgttttc gaaccgtcga
     3601 tccaatgttt tacaccctcg aagctgtgtc tttcacgcgc gatgccgtga ttcacgcccg
     3661 cgacgatgtc tgttgcaccc gcgatgctgc gttctgcacc cccgatggga tgttctgcac
     3721 ccccgatgag atgttctgca cccgcgatga gatgttctgc acccgcgatg agatgttctg
     3781 cgcccccgat gggatgttct gcacccgcga tgagatgttc tgggcccccg atgagatgtt
     3841 ctgcacccgc gatgagatgt tctgcgcccc cgatgggatg ttctgcaccc ccgatgggat
     3901 gttctgcacc cgcgatgaga tgttctgcgc ccccggtgag atgttctgcg cccccgatga
     3961 gatgttctgc actcacgatg ctgcggtctg cacccacgat gctgtggtct gcactcccga
     4021 ccctgtgatc tgcactcacg acgccgcgtt ctggacccac gacgctacgt tgtgaaccac
     4081 cgacggtgct tttcgaactt cggcagcgtc atcgggactt ggggctcctg agcggatgtg
     4141 ctggccttct gagctctctt tcgtggctgt acgatagcga tctccgcatc tcaggaggag
     4201 cccgaatgca cgccagccgc ctggcactca cgctgttttt cagctccgct gctctgacgg
     4261 tgcatcttgc cgcgcagccg gcactcacgt cgtggaagct gaacaccacc ggtgagaccg
     4321 gaacgtacac gctgcagaac gggacgaaag gcacccttcc agcgaacgtg cagctggtcc
     4381 agtacgacgc gacctacgtg tacgtgagtg cgagcggcat tccgtcatac gacatcggtc
     4441 catttccagg gaaccccaac gtcccggcaa accagcatta catcgccgcg ttcgctcgca
     4501 cgcccgtcgt gcctgcaacg aaggtcgccg cccaactcgg cgtcaacggc ctgtatgtga
     4561 acggcgtcgc aatgttcaac gcggacgacg gcaactcctg gcgaaacgcc agccgtaccg
     4621 agtcgccgca gggcgacggg atctggcacc gcaatggagg agtcgtcgag gcggtcggct
     4681 tcgacccgtg cgaaggacac ccgcagcagc agggcgtcta tcatcatcac gagtactctc
     4741 cctgtctcgg agcgcagctc ggcgccacga ccacgtcgca ttcgccgctg ctcggctttg
     4801 ctctcgatag ctttccgatg tacggcccgt acggctactc gtcgccgctc gatccgacga
     4861 cgggcgtgcg gagaatggtg agcggttatc ggctccgttc gatcacgcaa cgacacgcgc
     4921 tccccgacgg aaccattctt acccccgcgc aatacggccc cgacgtcgat tccaactttg
     4981 tactcggccg ctacctcgag gacttcgaat ggctggccgg actcggcgat ctggacgcgc
     5041 acaacggccg cttctgcgtg acgccggatt accccgcagg cacgtatgcc tacttcctta
     5101 cgctcgacga gcacggccag ccggccttcc cctacacaat cggcttgacg ttttgcggaa
     5161 cgccgaacat cggcacgcac gcgaccgttc cgcttacagg tgtgaccaca cttccggttc
     5221 tcgccatcac cagcttcgtg ccgccgtcag gcggagcagg gagcagcgtt gtcatcaccg
     5281 ggaagggttt cacaggagtg aaagcggtct acttcaacga ggttccgtca gcgagcttca
     5341 gcatcgtggg tgacacgcag atcaccgcga ccgtcccgag cggcggcgct gccggacgga
     5401 tccgagtcga ccgcgcggac gcgtacgcgc ggagtaccac cgacttccag ccctcgcagc
     5461 cgagacgccg cgcggccggc aagtgatgcg cgccccagac ccatcgaggc gggacgcctc
     5521 gatgcccgcg gtcgagacga ccgcactcct tgcgacaccc aagcctcgca gcgctgagga
     5581 gtgcggtcgt ctcgaccgcg ggcactcggg cgtcctcgcc cgagtgcggt aagggcggag
     5641 gtcgaaatcg ccgccgtcct tgcaagcgtt aactacggtt tcttcgagac gcggctcccg
     5701 tccttcgatg cgccgtacgg agtcacgtac tcccgttcga tgaccggagc attcttgtcg
     5761 tcgccgggct cgcggaccgc gggcaggata atgcgcggga tgggatcggt cgaggcggcg
     5821 aacattccct tccggctgct gaagtttccg gcgaacgtgg ccacgatcag atcgaggtcg
     5881 ggaactactg tcaccgtctg gccgccggcg ccgcgggcgg agtaggtgtg gaccgtccga
     5941 ttcttgtatg ggtagtcctc gccccaccag agatagccgt agtagatgtt gcgcaagtga
     6001 tagagcggcg cgccggcgcg tgcgacgaac tcctgactga cgatccggcg gccttcccat
     6061 gtgccgccgt tgagcatcaa ctggccgagc ttgatgaaat cgcgcagaag aagagccatt
     6121 ccgccgccgc cgtacggatg tcccgcggga tccagcgccc attcgtagcg gcggatcttc
     6181 attggcccgc cgatcagacg atcgaagaag gacgtcgggt cttcgtgcgc ggcgcggccg
     6241 agcatgccga gggcgagatt ggggctggcg ctgcaataga cggcattttc tcccggcggc
     6301 gtcgccatcg gaaccttcat cgtgtagcga tagaaatcgg gctccgcttc ctgttcccac
     6361 atcttgtctt cattgcccgg cgcgtcatcg ttcgtgtcgt cgcagaagta gcccgacgac
     6421 atcgtcaaaa ggtgctccag cgtcatggcg cgcttctgcg gctccaggtc cggcggaaac
     6481 gtgccgccgt tcatgacctg atagaccggg ctggagagct tcagcggaac gcctgcgtgc
     6541 attgcggcgc cgacgagtag agacgtcagg ctcttcgacg cggaacgggt gttgtggagt
     6601 aggtcgcggt cctgttcgtg gaagtactcc tccaggacca gctttccgtg acgggcgatg
     6661 agaacgccgt gaacctgggg cgcgtcctgc gaatccatcg gcgtgtcgag aatgctctga
     6721 acgaacttct cgatcgtagg ccggtcgata ttcgcttcat cgagcgtgct cacctgccag
     6781 ccgtcgtcgc gtgcgagcgg tgggcggtag atgtagcggc cgggttgctt gccgcgtggg
     6841 tagaacccgc tgtcgtcgcc gtcgcgcagg aagtcgtagc tcccgccgcg gccggggatc
     6901 gcgaaggtga tcacggcgtt ctccgcgtcg tacgtcccgc tcgccaccac ctgctctttt
     6961 ccgccgcgct tccccatcag tttcaaggcg ttgccgtcgc gcgtcagccg ctccacacct
     7021 tgctgggtgc cgaggtcgaa ctccggattg cgcaggacgg cgcggaacga gccatcgggt
     7081 tgcggttgca gaaggagata aaaagtaaag gtctcctcga tcgggtcgac gttgccgcgc
     7141 catcgattcg gaccgtcagg cttcaacacg accggagacg cgctgatggg cgttgccttc
     7201 caatacaagg ccgttccggg ccgcagccaa tggccgcgga tgacgccctg atcatcgcgt
     7261 cgtccacgga acgtcccacc gttgtccggc agcttgaacg tgagctcgcc gttggcgaca
     7321 gtcaccggaa cgatccggcc ggccatgtcg gcggtccatg cgttcccgct ccgttgcaac
     7381 acgagcgcgc cgcgcgcgtc gggaccgaat cgcttcttgg ctttccacag tcccgccagc
     7441 tcattcgcgg aaccagttgt ctcggccaga agcgacgctc ctacgaacaa cagaacgaac
     7501 gtgatggcag cgatgcgagc gaaccaacga ctgcgaatga tcggcgaggc gtgtttcatc
     7561 gtgtcgtagc aatgttagac gccgggtgga cgccttcgtt agtaagcgcg ctgatgcgta
     7621 gcgagtcaat tcggaaaacc attcaccacg gagacacaga ggacacagag ttggactcga
     7681 tcgcgatgtc gtcaatcgac gcttcgctgc tgaaaatgtc gactgcgagg tggccgatcg
     7741 agacttcgtt gtggaaaatc tcgacttcga tgtggaaaat ctggacatcg cagtggaaaa
     7801 tgtcgacatc gcagtggaaa acctggacat cgcagtggaa aatcggcact gcgatgttga
     7861 aattctggac atcgcagtta gaaatctgga catcgatgtc aagactcacg acatcgatgt
     7921 cgaaaatctg gacatcgcag tcagagatct ggacatcgca gtggaaaatc tggacatcga
     7981 tgtcgagaac ctcgatctcg ttgtggttaa tttgcactta gttgtggtta atctgcactt
     8041 cgatgtcgac gacagacgcc cgactggtcg ttttgagtag tggccgatcg cgcccgttca
     8101 gctgtgtggt gtcttcgcgg cttcgtaaga cgaagttgag gacgcggcgt ccgagcaggc
     8161 cagtccgacg agccgatcgg caaaacaacg ctccggctcg ggcgggatga gcttacggat
     8221 tcgcgctgcc gccgccgcgc cgacttcgcg ccaccgatca cgctcgttcc aggcgcgctc
     8281 catcgcctcg tcgagcgcat cttccgtcgc tgcggcggcg acgtagccgc tcgccccatc
     8341 ctcaaccacc tcggcgttgc cgcccacatc ggtcacgatc gccacccgcc cggacagcat
     8401 cgcttccacc agcacgatcg gcaatccctc gcatcgcgac ggaagaatca ggccatggtg
     8461 gccgctccag atcgcgggga catcgtgtac gaagcccgcg aaggtcacgt tcgtgaggcc
     8521 gaggttctgc gccatccgct ccagactggc gcgctgcagg ccctcgccgt agaacgtcac
     8581 cgcgagcggc cgatcgcgcc agtgccgtcg cgccagaacg cgcagcagaa gatcctgccc
     8641 cttctccttt gggtgcagac gtccgatgca ggccagtcgc cattcgttcc cgtcgtccgg
     8701 ccagtccgtg cgcggctccc acggaacgag aaacgggttc cgcaccaccg ctgcccccgg
     8761 aagcggaaat ccgagctgct cctccgtcag ccggcggctg tgctcggaga cgaaaaacca
     8821 agccaccgcg gcgcggtaga cggcggcgat cgtcttctgc tgcccatcgg ccggccaata
     8881 gagctcgctc gctttgtgcg acacgatcgc gtacggcagg cggaggcggc ggcacacctc
     8941 ggcgagaacg tatccgtcgt ggttgccacc ctgcgaaacg agcacgagat ccgggcggcg
     9001 cgagagcgcc aatccgatca ggagccgcat ggcctcgtgc ccgtaggcag cagcggacga
     9061 caacgtgcga acgagcgtga acaggcgcct gggcatgagt gggaagcggg ccagatcgcg
     9121 aacgcggcat gagagcgcgc gcaagcgccg gatgcgcggc tcggattcat cgacgccgct
     9181 cttgaagacg gtcactgtgt gcccatccgc cgccagcacg gcagcggccg cgctccacaa
     9241 ctcctcgctg ccgccccagg cccggtttcc gctgatgaag acgacattca ttgcgctatt
     9301 ctcgttttat tctgcgcgat cgcctcggcc atatcgttaa tcccatcgtc gatctcacga
     9361 gagcggacca gtggtgggag tacaagctcg tcacgatttt gtcggccttc tatgcgaccg
     9421 cgctcgtcct gcacgtcgcc gtgagctcgc tgtgggtcag cgcgctgtcg ctgctgctcg
     9481 cgatcgtcgc ggccgcgatt tacgccagcg tgatcaacga cgtcaccgac ctggccggcg
     9541 accgcgaggc ggggaaacgg aatgccatgg ccggccgctc ccgctcgatg gtggcggcgc
     9601 tcgtcgcgct ttccgccgcc gcaggctccc tcttcgcctg gctctggcgc gatgacacgc
     9661 cactcctctg gtcttacctg gccacgtggc tggcattctc gctctattcg cttccggcgt
     9721 ttcggctcaa ggagcgtgga gcggcgggcg tcctgtgcga cgctgccggg gagcaactgt
     9781 tccccgcgct ggtcgcggtg ttcctggcca gccgcggggc gcaccgcgcg gtgggtgtgg
     9841 cctggatggc cagcgtcgcc gtctgggcgc tcgcgtatgg cctgcgcggc atcctctggc
     9901 atcagttgac cgatgaagac aacgatcgcg ccgccggctt tcggaccttt gtgtgccgtc
     9961 atccgcgcgc ggcacccgcc atcggcacgt tcatcgtttt ccccatcgag ctcggcgcgc
    10021 tcgcggccat gctgtggcag atcgatagcg cgtggccgcc ggcgtttctc gtgctgtacg
    10081 cgctctacgc ggttctcagt gcccgccgct ggcaaacatc gccggtgatc gtggtgccgc
    10141 agccgcgctt cttcatcgtc ctgcaccagt tctattccga tctgtttccc gtagcgctgc
    10201 tcatcgccgg ctccgttcgc gaccggcgag tgctcatcgt tctggccgtt catctcctgc
    10261 tgtttccgcg gcgcgtgatt catgcgatct gtcggctcag ggcctcgact gcgaggaccg
    10321 tcgtcaacgc ctcggagccc caccacggcg gcgtcggctg aaactccttc tttgagatcc
    10381 gctcgaggcc cgcgtgatag aagccggcgc tgggccaggc tcccgaggga agttgcgcgt
    10441 cgatcagagg ctggaggtca ggcgtgcgat tccacagttg cagagccgac gcagcgagtg
    10501 cgagctcgag cgcgttgcgc ggagtcgcgg cctcgatgcg cggcacgatc atcccgcccg
    10561 cctcgggtgc gatcccggca agcgcgtgcg agaaaaagta ccagacggtg aaccggctct
    10621 cgtaccactt ggtggacctc atttcatcat tggcgcgcag caccgcgagc atatgcgcaa
    10681 tggccggccg cgtctctttg cagtcgccga ggtagatgac gacgttggcg ttgatcacgg
    10741 catcgacatc gcgtatctcc gccgtggtct cccggaagaa gcgatagatc ttgcgtggat
    10801 ggggccacca tcgcacgatc cacgttcgaa agagaccgct gcgctcgcga ttggccagca
    10861 gcagggcgcg gttgtccggg aagcgttgtc cggcagccgc aagtgctgcg gacgcgatcg
    10921 acgtgtcgtc gacatcgagc ggaatgtggt cgtgtcccgc gtgctcggtc gacggatagc
    10981 gccacagacc gtcgggctcc atttcgcgca acaggaaatc ggtggcgcgc gaccgtacgc
    11041 gcgcggcgga tggtgtgatc gacaacgccc gcgccgcgag cgctgttgta aagacgaccg
    11101 gatcacgaac gcag
//
LOCUS       DATLVW010000075         7524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_169820, whole genome shotgun sequence.
ACCESSION   DATLVW010000075 DATLVW010000000
VERSION     DATLVW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169820"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VNN25_02530"
     CDS             complement(<1..452)
                     /locus_tag="VNN25_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90430.1"
                     /translation="MKRTLTACFVFLLFGCATTPRTAVRVDVEEQPDARHWRVHYEFP
                     RPVSEISFPNTRAPFRGTRWTIVEPAGATWKGLWQHDTIVFRSPTTHTTVEFADDFSE
                     REKDYLLNVAFTDGSRLLYTGHLRVSDEPHVWHFQTAANRTIRVADRSS"
     gene            546..1118
                     /locus_tag="VNN25_02535"
     CDS             546..1118
                     /locus_tag="VNN25_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH90431.1"
                     /translation="MTELALRHATYEDLLKVPDNLVAELIDGELYTSPRPGRDHARTS
                     SVLGARLTPPFDIGDGGPGGWWIIDEPELHLGRHALVPDLGGWRRERTPEYPNTSGCE
                     VPPDWVCEVQSPGTARIDRVKKLPIYAEHAVQHIWLIDPATQTLEVLRLEEGRWVIAG
                     NYGGDDVVRAEPFEAIELALSALWIEPAAK"
     gene            1198..1770
                     /locus_tag="VNN25_02540"
     CDS             1198..1770
                     /locus_tag="VNN25_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH90432.1"
                     /translation="MTDLVLRCATYEDLLKVPDHLVAELIDGELYTSPRLAPRHSNVM
                     TLLGSDLNVRFQRGRGGPGGWWILDMLQLRLAAESLVPDIVGWRRERLPNLPDTATID
                     VAPDWVCEVQSPSTALFDRVKKLPVYAGQGVQHVWLVDPAVRTLEVLRLENRRWILLG
                     NYSDDDIVRAEPFEAVQIELGALWIDGPAS"
     gene            complement(1789..5316)
                     /gene="smc"
                     /locus_tag="VNN25_02545"
     CDS             complement(1789..5316)
                     /gene="smc"
                     /locus_tag="VNN25_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXH90433.1"
                     /translation="MFRLDKLEIHGFKSFYDPAHLAFPAPMTAVVGPNGCGKSNICDA
                     IIWVLGENRASHIRGETMDDVIFQGSARRRPLSMGEVTLTLATVNGDTDHPASDDGRI
                     TINRRVFRTGESEFRLNGKRVRLKDISDILMDTGLGIRNYSVMEQGKIDLILSNKPQD
                     RRKLIEEAAGITKYKTKRRAAELKLEETQQNLLRIDDTLSEVTRNLNSLKRQAGKARR
                     HKELSEQLSTAKRALYKGRIVAAQAEQAAAEEGMAAAQASESELAAQLATDEAELAEN
                     RTTLSSRSSHASHVREEMASLSGDVERLRSFLQQSETNLGDLIQRSENAQMQIAQLET
                     EAAEQQALLDERAQALEVARGERNALREIAEQTEKDRQERSDEVRRHEKALADAREVL
                     MRTIARISEARNQLHQIEVAVEKCEFYLGKLSESARKATESRDGALTLSADWDQRVQE
                     AEGALETARQTAADAISRNDELTIRRNELRDTLAGARDRVSQMTYKLDSLRTLLVSLE
                     SQDEDVRRAILELIPNATPAAEAVRATEGFEAALDTLLRDVSKAVVVEDARTVIDAIG
                     RLRERGAGRGAFITLDFVASGEGGRRGDAKYAVVGDGEIADAVRRAMPEAYIVGSLGE
                     ALDRAEERPSATFVTLEGDVVRGPLVIGGKSANAVPGVFSLKRELSDLETLLGTEETR
                     ASGIAGDLQRIEDDVRGADDARILAEERARAADQELRERRGNRERAKQELDRFERDLA
                     VASEEQTLYLEEKEQLIARKNAAIEELRQLEQQEAECQDQIRQHEEQLTILRSTFEAV
                     TEAASKARVDVEAAAGNVNAIQREHENLSRIVVSLGARATSLLQEIESLARKRQETEA
                     AVESSRGQLDESLRKLHDLSEVRVEIEAEVADLQVRVQELETRAVASREQWNTAKDSL
                     FESERRSDRATAAFDLLREQIALDLHAGIDALAEVEPPSDDEGRAALETEVAKLTDQI
                     EKVGPVNVLAIDEHQELEQRESFLRTQRDDLVQAIESLRSTIRKINLTSRELFRDAFH
                     AVNEYFTEIFISLFGGGTARMQLLDEEDVLESGIELVAQPPGKKNQSIALLSGGERAL
                     TALALLFAIFRYKPSPFCILDEVDAPLDEVNNERFVRLVRDMSRDTQFIVITHSRRTM
                     EAADVLYGVTMEEAGCSRLVSVNFAEVEA"
     gene            5507..6553
                     /locus_tag="VNN25_02550"
     CDS             5507..6553
                     /locus_tag="VNN25_02550"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXH90434.1"
                     /translation="MTLDDFLQHEIDVGSFPSAAYAIGSSRGIERENALGHAVAVPMR
                     IPATLDTLYDCASITKPLITTTLALQLLDLDPYVRSLLTHTSGMRAWLPLYLYDNYLD
                     AIRDHGPEYEPGTRVIYSDLNFVLLWSMLPNFVELARERILAPLGIEHDAMFNPPPSQ
                     RPRIAATEWGQRYEAKMAAVSGQRSEIRDQRHSDQRSAVSGQGQRLFAQRDGLIWGET
                     HDGNSHFAGGTAGNAGLFATARAIFRLAQSWTNAELLPRALVDEATSNLTPNLEDARG
                     LGWQKPTGSLATSMLTDRAYGHTGFTGTSVWIEPERDRIMVLLTNRVHPCAAPIAMQR
                     IRGEFHRWALTYNE"
     gene            6952..>7524
                     /locus_tag="VNN25_02555"
     CDS             6952..>7524
                     /locus_tag="VNN25_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH90435.1"
                     /translation="MILRSVHVENFKSVKDSTVFTIDEKVTCLVGKNESGKTAILQAI
                     TKLNPTEPSLARFDEMEYPRHKLNEYQESEEEANVLTTVWALESDDQEALAAVLGPSV
                     KTLETITVSKGYDNEQVYSVVIDEEAVVEHLIDSHELLDDERTTLDDATTIAQLRKSV
                     DEIDDPSQRQRNLLLTLSKSFEKKSAVIVAM"
BASE COUNT         1356 a         2357 c         2330 g         1481 t
ORIGIN      
        1 gagcttcgat cggccacacg aatcgttcga ttcgcggcgg tctgaaaatg ccagacatgc
       61 ggctcgtcgc tgacgcgcag atggccggtg tacaggaggc gacttccgtc ggtgaaggcg
      121 acgttcagca gatagtcctt ctcccgctcc gagaagtcat ctgcaaactc gactgtggtg
      181 tgcgtcgtcg ggctgcggaa aacgatggtg tcgtgctgcc acagcccctt ccacgtggcg
      241 ccagcgggct cgacgatcgt ccagcgggtg ccgcgaaacg gtgcgcgcgt attcggaaag
      301 gagatctcgg agacaggacg cggaaactcg tagtgaaccc gccagtgccg cgcgtccggc
      361 tgctcctcga catcgacacg tacggcagtg cgaggagtcg tcgcgcagcc gaagaggagg
      421 aaaacgaagc atgcggtgag ggttcgtttc atttctagtt tgacgagcgc cgaggagttg
      481 aagttacaca gaggcattat tgccaacaac gtgatcgagt gtgatcgccg aggtatcatt
      541 tcgacatgac ggaactggca ctccggcacg cgacctatga ggacctgctg aaggttcccg
      601 acaacctcgt cgctgagttg atcgatgggg agctgtatac gtcgcctcgg ccgggccgag
      661 accacgcacg cacaagctca gtattgggcg cgaggctgac cccgcccttc gacatcggtg
      721 atggcggccc gggtggatgg tggatcatcg acgagcctga gttgcacctg ggacgccatg
      781 cgcttgttcc cgatctcggc ggatggcgca gagagagaac gcctgagtat ccgaacacct
      841 caggttgtga ggttcctccc gattgggttt gcgaggtcca atcgccggga accgctcgaa
      901 tcgatcgcgt gaagaaacta ccgatctacg cagagcatgc ggttcaacat atttggctga
      961 ttgacccggc cactcaaacc ctggaggtgc tgcgtctcga agaaggccgc tgggtcattg
     1021 cgggcaacta tggcggcgat gacgttgttc gtgccgagcc attcgaagca attgagcttg
     1081 cactctccgc gctgtggatt gagcctgccg cgaagtaaga ctccctcgca gtagcctcga
     1141 tcaaatgatc gtggcggctt cgtgtgagtg gcaataacgc cgaggtacca tttcgacatg
     1201 acggatctgg tactccggtg cgcaacctat gaggacttgc tgaaggttcc cgatcatctc
     1261 gtcgctgagt tgatcgatgg agagttgtat acatccccgc gactggcccc tcggcattca
     1321 aacgtgatga ctttgctggg gtccgatcta aacgttcggt ttcaacgggg acgcggaggg
     1381 ccgggcggct ggtggattct cgacatgctg cagctacgtc ttgcggctga atcactcgtg
     1441 ccagacattg tgggttggcg gcgtgagcga ctaccgaacc tcccggacac ggcgaccatc
     1501 gacgtcgctc ccgattgggt ttgcgaggtt cagtcgccga gtactgcgct cttcgaccgc
     1561 gtcaagaagc tgccagttta cgctgggcag ggagttcagc atgtgtggct ggttgacccg
     1621 gccgtcagga cactcgaagt cttgcgttta gagaaccgtc gatggatcct gctcggcaac
     1681 tacagcgacg acgacatcgt gcgcgctgag ccgttcgaag cggtccagat tgagcttggt
     1741 gctctctgga tcgacgggcc tgcttcgtag ggattgatcc cctagaccct acgcctcaac
     1801 ctccgcgaaa tttactgaca ccagccgcga acagcctgct tcttccatcg tcacgccgta
     1861 gagcacgtcg gcggcttcca tcgtgcggcg gctgtgcgtg atcacgatga actgggtgtc
     1921 gcgggacatg tcgcgcacca ggcgcacgaa gcgctcgttg ttcacctcat cgagcggtgc
     1981 gtcgacctcg tcaaggatgc agaacggcga cggcttgtag cggaagattg cgaagaggag
     2041 cgccagcgcg gtcaacgcgc gctcgccgcc ggagagcagg gcgatcgatt gattcttctt
     2101 gcccggtggc tgtgccacca gctcgatgcc gctttcgaga acgtcttctt cgtcgagcaa
     2161 ctgcatccgt gccgtgccac cgccgaagag cgagatgaag atctccgtga agtactcgtt
     2221 caccgcgtgg aacgcgtcgc ggaacaactc gcgcgaggtg aggttgatct tgcggatcgt
     2281 gctgcgcagc gactcgatcg cttgcaccag atcgtcgcgc tgcgtgcgaa ggaacgactc
     2341 gcgctgctcc agctcctgat gctcgtcgat agccagcacg ttcactggtc cgaccttctc
     2401 gatctgatcg gtgagtttcg ccacttcggt ctcgagcgcc gcgcgccctt catcatcgga
     2461 cggcggttcc acctcggcca gcgcatcgat gcccgcatga agatcgagcg cgatctgttc
     2521 gcgcagcagg tcgaacgccg cggtggcgcg gtcgctgcgc cgctccgact cgaacagcga
     2581 atccttggcc gtgttccact gctcgcgcga cgccaccgcg cgtgtctcca gctcctgcac
     2641 gcgcacttgc agatcggcca cttccgcttc gatctcgacg cgcacttcgc tgagatcgtg
     2701 cagtttccgg agcgactcat cgagctggcc gcgcgagctt tcgaccgcgg cctctgtctc
     2761 ctggcgcttg cgggccagcg actcgatctc ctgcagcagg ctcgtggcgc gggcgccgag
     2821 ggagacgaca atgcgcgaga ggttctcgtg ctcgcgctgg atagcgttca cgttgccggc
     2881 cgcggcctcg acatcaacac gcgccttcga cgccgcctcc gtcaccgctt cgaacgtcga
     2941 gcgcaggatc gtgagctgtt cctcgtgctg ccggatctga tcctggcatt ctgcttcctg
     3001 ctgttcgagc tggcgcagct cttcgatcgc tgcgttcttg cgcgcgatga gctgttcctt
     3061 ttcttcgagg tagagcgtct gctcctcgga tgccaccgcg aggtcgcgct cgaagcggtc
     3121 gagctcctgc ttggcgcgct cgcgattgcc acgccgctcg cgcagctcct gatccgcggc
     3181 acgcgcccgc tcctcggcga ggatgcgcgc gtcatcggcg cctcgcacat cgtcttcgat
     3241 cctctgcaag tctccggcga tgccgctggc gcgcgtctct tccgttccga ggagcgtctc
     3301 gagatcggac aactcgcgct tcagcgagaa gacgccggga actgcattcg cggacttgcc
     3361 gccgatgacg agcggcccgc gaacgacgtc gccttccagc gtgacgaagg tcgcgctggg
     3421 ccgctcttcc gcgcgatcga gtgcttcgcc gagcgagccg acgatgtacg cctcgggcat
     3481 ggcgcggcgc acggcgtccg cgatctcacc atcgccgacg accgcatact tggcgtcgcc
     3541 gcgccgtccg ccttcgcccg atgcaacgaa atcaagagtg atgaaggcgc cgcgtccggc
     3601 gccgcgctcg cgaagccgtc cgatggcgtc gatgaccgtg cgtgcatctt cgacgacgac
     3661 ggccttcgag acatcgcgca gcagcgtatc gagcgccgct tcgaatcctt ccgtcgcgcg
     3721 caccgcctcg gccgccggtg tcgcgttcgg gatcagttcg aggatggcgc gtcgaacgtc
     3781 ctcgtcctgc gactccagtg acacgagcag cgtgcgcagc gagtcgagct tgtacgtcat
     3841 ctgcgagacg cggtcgcgcg ctccggcgag cgtgtcgcgc agctcgttgc gccgaatcgt
     3901 cagctcatcg ttgcgggaga tcgcgtcggc cgccgtctgg cgtgcggtct cgagcgcgcc
     3961 ctcggcctcc tgaacgcgtt gatcccaatc ggcgctcagc gtcagtgcgc catcacggct
     4021 ctccgtcgct ttgcgcgccg actcgctcaa cttgccgaga tagaactcgc acttctcgac
     4081 ggccacttcg atttgatgca gttgattgcg cgcctcggag atccgggcga tggtgcgcat
     4141 caatacctcg cgcgcatcgg ccagcgcctt ttcgtggcgg cgcacttcgt cggagcgttc
     4201 ctgccggtct ttctccgtct gctcggcgat ctcgcgcagg gcattgcgct cgccgcgcgc
     4261 gacctcgaga gcctgcgctc gttcatcgag cagcgcctgc tgttccgctg cttctgtctc
     4321 cagttgcgcg atctgcatct gcgcgttctc gctgcgctgg ataagatcgc cgaggttcgt
     4381 ttcggattgc tggaggaacg agcgaaggcg ctccacgtcg ccggagagcg acgccatctc
     4441 ctcgcgcaca tgcgacgcgt gcgacgagcg cgaagacaat gtcgtgcggt tctcggccag
     4501 ctccgcttcg tctgtcgcca gttgcgccgc cagctccgac tcgctcgcct gcgccgcggc
     4561 catcccttcc tcggcagccg cttgctccgc ctgcgcggcc acgatgcggc ctttgtacag
     4621 cgcgcgtttc gcggtcgaga gttgctcgct cagctccttg tgccggcgcg ctttgcccgc
     4681 ctgccgcttg agcgaattga gattccgcgt gacctcggag agcgtatcgt cgatgcgcaa
     4741 caggttctgc tgcgtctcct ccagcttcag ctccgcggcg cgccgcttcg ttttgtactt
     4801 cgtgatgccg gccgcttcct cgatcagctt gcggcgatcc tgcggcttgt tggaaaggat
     4861 cagatcgatc tttccctgct ccatcaccga gtagttgcgg atcccgagtc cggtatccat
     4921 gaggatgtcg gagatgtcct tgaggcggac gcgcttgccg ttgaggcgga actccgactc
     4981 gccggtgcgg aagacgcggc ggttgatcgt gatgcggccg tcgtcggagg caggatggtc
     5041 ggtgtcgcca ttgaccgtcg ccagcgtaag cgtgacctcg cccatcgaca gcgggcggcg
     5101 gcgcgccgag ccctggaaga taacgtcgtc catcgtctcg ccacgaatgt gggaggcgcg
     5161 gttctcgccc aggacccaga tgatggcgtc gcagatattc gacttgccgc agccgttggg
     5221 gccgacgacg gccgtcatcg gagcggggaa ggcgaggtgc gccgggtcgt agaacgattt
     5281 gaagccgtga atttctaact tgtcgagacg aaacattgaa tggaaagcac ctgacggatc
     5341 gatttgaacg cgccggatgg tagcagcaat ccccaggcgc ggatgggtgt gaattgcctg
     5401 gtcgtaaacc attcacggca aagggatatt cgcggttgac acggcagtgg cgcaatcgtt
     5461 cctgtgcatg tgcggcccgg gccatggctc atacaatggc gccgcgatga cgctcgacga
     5521 ttttctgcag cacgagatcg atgtggggag tttcccgagt gcggcctacg cgatcggctc
     5581 ctcacgcggc atcgagcgcg agaacgccct cggccacgcc gtagcagtgc cgatgcgcat
     5641 cccggccacg ctcgatacgt tgtacgactg cgcgtcgatc accaagccgc tcatcacgac
     5701 gacgctcgcg ctgcagttgc tcgatctcga tccctacgtt cgcagcctgc tcacgcacac
     5761 ctcgggcatg cgtgcatggc tcccgctgta tctctacgac aactacctcg acgcgatccg
     5821 cgatcacggc ccggagtacg agcctggcac gagagtcatc tactccgacc tgaatttcgt
     5881 gcttctctgg tcgatgctcc ccaacttcgt cgaactcgcg cgcgaacgaa tcctcgcccc
     5941 cctcggcatc gagcacgacg cgatgttcaa tccgccaccg tcgcaacgcc cgcgcatcgc
     6001 cgcgacggaa tggggccagc gatacgaggc gaagatggcg gcggtcagcg gtcagcgatc
     6061 agagatcaga gatcagcgtc acagcgatca gcgatcagcg gtcagcggtc agggacaacg
     6121 actgttcgcg caacgcgatg gtttgatctg gggcgaaacg cacgacggca actcccactt
     6181 cgccggcggg accgcgggca acgccggcct cttcgccacc gcccgcgcca tcttccgcct
     6241 cgcccaatca tggacaaacg ccgaactgct cccgcgcgcc ctcgtcgacg aagccacctc
     6301 caacctcacc cccaacctcg aagacgcccg cggcctcggc tggcagaaac cgaccggcag
     6361 cctcgccacc tccatgctga ccgaccgcgc ctacggccac accggcttca ccggaacatc
     6421 cgtctggatc gagccagaac gcgatcggat catggtattg ctaacgaatc gcgtgcatcc
     6481 atgcgccgcg ccgatcgcga tgcagaggat acgcggggag tttcatcggt gggcgttgac
     6541 ttataacgag tagagtgtaa ggtcttcatg cgtacaacgc tcgacgtacc ggaacccctg
     6601 ctggacgaag ctcgcctgct tcttagtgtt gggcgccttc tacgtcgacc agcagaaaca
     6661 ggggtcttgg gtttgcggtg gcgcacctct tcaacggcga gcctcacgac tatgtcctga
     6721 tttcttgata cgggtgcgga gacccgacga attcgaggac atgctcattc cctgagacga
     6781 agggatacga tccacgccag gacgtgaagg ctacggcggc tgagcggtga gtgaaggtgg
     6841 taaatgcaga cgattcctcg gccgctgacg atatcgcgtg aaaaagatgc cagagatcag
     6901 tgaagtcatc tcaacggtcg cggaaactgc cgcggcaacg gagtcgagcg catgattctt
     6961 cgatcggtgc acgtagagaa tttcaagagc gttaaggatt caactgtttt cactatcgat
     7021 gaaaaggtga cctgcctcgt tggcaagaac gagtctggca agacagcgat cctgcaagcg
     7081 attacgaaac tgaacccgac agaaccttca ctcgcgaggt tcgatgagat ggagtacccg
     7141 cgtcacaaac ttaatgagta ccaagagtca gaggaagagg cgaatgtcct gacgaccgtt
     7201 tgggcgctcg agagcgacga tcaagaggcg ctcgcggcag tactcggacc ttccgtgaag
     7261 acgctcgaaa ccatcacggt gtcgaaggga tacgacaacg agcaggtgta ctcggttgtg
     7321 atcgatgaag aggcggtcgt agagcacctg atcgactcgc acgaactgct cgacgacgaa
     7381 cgcaccacgc ttgacgatgc gaccactatc gcccagcttc gcaagtcagt tgatgagatt
     7441 gatgaccctt cacagcggca gagaaacctt cttttgacgc tgagcaaatc cttcgagaag
     7501 aagagtgcag tcattgtcgc gatg
//
LOCUS       DATLVW010000076         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_335793, whole genome shotgun sequence.
ACCESSION   DATLVW010000076 DATLVW010000000
VERSION     DATLVW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335793"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VNN25_02560"
     CDS             complement(<1..503)
                     /locus_tag="VNN25_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HXH90436.1"
                     /translation="MTSPYAQSLWNDLFIPGIPYLEKALRTLLVYVAVLLGLKLAGKR
                     ELSQLNPFDLVVLLLLSNTVQNAIIGNDNSVAGGLFGAAVLLLTNHFLVRYLFKSGRL
                     DKVEGSPDVLISDGRILRDRLDREMITVPELEAAARKQGINSVAEIKECRLETGGALT
                     FVTRVPTL"
     gene            complement(725..>2003)
                     /locus_tag="VNN25_02565"
     CDS             complement(725..>2003)
                     /locus_tag="VNN25_02565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH90437.1"
                     /translation="RIAFTGALNAKPLRFYNQGDLFIVDAVPGAQPRNLTTSYDFSIE
                     DGLSGDQHPPRAASGGGIVWSRDGKSVVVVTAENGRANLKRFSTTDGKVDSVTTGAND
                     VEAYSASADGSKIVTLVSTPTNIGDLFVVDANTPRAAPRQITRVNEGLLSTLDLSAPE
                     EFWYNSFDGKRIQGWILKPPHFDASKKYPLILEIHGGPNAAYGQTFTHEFSWFAAKGY
                     VVLYTNPRGSTSYGQDFANIIQFHYPGDDYKDLMAGVDEVLKRGYVDSTRMGVTGGSG
                     GGVLTNWTITQTHRFAAAVSQRSIADWSGFWYTADFTLFNPTWFRAAPWEDPKGFAQV
                     SPITYVANVTTPLMLIEGEADMRTPPADGGEQMFRALKYLHKPTVMVRFPDETHELSR
                     SGKPWHRVERLRHIVGWFDVYLSHISNSDYEVH"
BASE COUNT          396 a          572 c          618 g          417 t
ORIGIN      
        1 agagtcggga ccctcgtgac aaacgtcagc gcgccgcccg tctcgagcct gcactccttg
       61 atctccgcca cagaattgat tccctgtttg cgcgcggcgg cctccagctc ggggacggtg
      121 atcatctccc tgtcgaggcg atcgcgcaga attctgccat cgctgatgag tacatcggga
      181 ctaccctcga ccttgtcgag cctccccgac ttgaacaggt agcgaaccag aaagtgattg
      241 gtgaggagaa gaaccgccgc gccgaacaat cctccggcca ccgagttgtc attgccgatg
      301 atcgcgttct ggacggtgtt cgagagaagg aggaggacga cgaggtcgaa cgggttgagc
      361 tgcgagagct cgcgtttgcc cgccagtttg agtccgagca ggacagcgac gtagacgaga
      421 agggtgcgga gagctttctc gaggtacggg atgcccggga tgaagagatc gttccagaga
      481 gattgtgcgt agggagaagt cattttcctg tcgccgttgg tgggatttgt gcgggcagag
      541 taggcatcca caaaaccaaa gcggggagct gacagcttta cagctaatgg ccaggagcct
      601 tcatactggc gatctgaagg gagccggcga ttcaaccatc cgaaatggcc tacgcctgta
      661 tgagagctct gagctatcag ctttggttgc tgtcggctcc ccgcttcagt actcgatgtg
      721 ccagtcaatg cacctcgtaa tcagaatttg aaatatgaga gaggtacacg tcgaaccatc
      781 caacaatgtg tcgtagtcgc tccacccggt gccacggttt tccggagcgg gacagctcgt
      841 gcgtctcgtc ggggaagcgg accatcacgg ttggtttgtg cagatacttc agagcgcgga
      901 acatctgctc gcccccgtct gccggtggcg ttcgcatgtc agcctcgccc tcgatcagca
      961 tcagcggcgt cgtcacattc gcgacgtagg tgatcggcga gacctgggcg aagcctttgg
     1021 gatcttccca cggcgcagcg cggaaccagg tcggattgaa gagcgtgaag tccgcggtgt
     1081 accagaagcc cgaccagtcg gcgatcgaac gctgtgacac ggctgccgcg aagcgatgtg
     1141 tttgcgtgat cgtccagttc gtcagtacac cgccaccgct gcccccggtt actcccatgc
     1201 gcgtcgagtc aacgtaccca cgcttgagaa cctcgtcgac gcctgccatc agatccttgt
     1261 agtcgtcgcc cggataatgg aactggatga tgttggcgaa atcctgtccg tacgaagtgc
     1321 tgccgcgcgg attggtgtag agcacgacgt agcccttggc ggcgaaccag gaaaactcat
     1381 gcgtgaatgt ctggccgtaa gcggcgttcg ggccgccgtg gatctcgagg atgagcggat
     1441 attttttcga agcatcgaaa tgcggcggct tgagaatcca gccctggatg cgtttgccgt
     1501 cgaagctgtt gtaccagaat tcctcgggcg cgctcaagtc gagcgtgctc aacaggcctt
     1561 cattgacgcg cgtgatctgc cgcggggcgg cccgaggcgt gttggcatcc acaacgaaca
     1621 aatcgccgat attggtgggt gtcgatacga gcgtcacgat cttcgatccg tcggcgctgg
     1681 cactataggc ttcgacgtcg ttggcgcccg tcgtaaccga atcgaccttt ccgtcggttg
     1741 tcgagaagcg cttgagattc gcgcgtccgt tctcggccgt cacaaccacc acgctctttc
     1801 cgtcccgact ccatacgatt ccgccgccgg aagccgcgcg cggcggatgc tgatcgccgc
     1861 tcagtccatc ttcgatggag aaatcgtagc tcgtggttag gttgcgcggt tgagcaccgg
     1921 gcactgcatc cacgatgaat agatcaccct gattgtaaaa gcggagcggc ttcgcgttca
     1981 gggctcccgt gaatgcgatg cgc
//
LOCUS       DATLVW010000077         3052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_250782, whole genome shotgun sequence.
ACCESSION   DATLVW010000077 DATLVW010000000
VERSION     DATLVW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250782"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VNN25_02570"
     CDS             <1..226
                     /locus_tag="VNN25_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXH90438.1"
                     /translation="FGTPIINQPQVAILGVGGVKKRPVVVETKEGDVIAIRSMCILAL
                     TFDHRLIDGAVADQFMARLRSLIEAGQFTL"
     gene            complement(275..1813)
                     /locus_tag="VNN25_02575"
     CDS             complement(275..1813)
                     /locus_tag="VNN25_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HXH90439.1"
                     /translation="MPADQSASFSLANVIRAHGVGVRGLDVPRRSEQYEGRFGRMFRS
                     LPSADHERADLITLANRMIADPEPDPGDATPDPEENRGISAGYTYVGQFIDHDITFDP
                     ASSLQKTNDPDGLVDFRTPRFDLDSVYGRGPDDQPYLYESDGIHLLHGTRLLTGNPGD
                     PNTFDLPRDTRDAGERRAIIGDPRNDENVIVSQLQGIFIRFHNRIADLLGGSAKFADV
                     QRVVRWHYQWAVLHDFLPTIAGWEMVRNVLPHLRKDGKDKSPYDEKPDLRFFHWRDSP
                     FMPIEFSVAAYRFGHSMIRPQYRLNSTLPNRFPIFPDLRGFHEFPTPWAIEWPLFFHI
                     HGGAPDTGPTRVQPAYKIDTSLVDPLRNLPFITDGIPSLAQRNLLRGLTFGLPSGQTV
                     ARHMCIDPIPDDQLKCGKLTEEDFPNNPKLIDISPNFADNAPLWFYILAEPQLQFEDD
                     DTPITLGPVGGRIVTEVIAGLMTGDNHSYLSQQPCWRPIKELTRSGHFGFAELVLVAT
                     GKLP"
     gene            1907..>3052
                     /locus_tag="VNN25_02580"
     CDS             1907..>3052
                     /locus_tag="VNN25_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HXH90440.1"
                     /translation="MSRWIALLGLVLAVSAYGQAISPVVKNSHAAAATSRPIDARIPF
                     PAANETWLRARSKHFVFISSADENRTRAIAAELETLAAALTKVDATFSTSSGPATRVI
                     VFTNPRESRPYFDMLRDRPNSDVSGVFVSQRDGGTMLINDGSTWRGGDRPPLHELVHD
                     LLESGGARIPLWLDEGLAEYFSNATIRRGSVSAGEPMRNHIATLRQRRRIPPAELFVT
                     TRESDTYNLPPKQDVFYAESWAIVDWLVRSASSSGAEFYGFVHDVANGIDVETALQLR
                     YHRSLRDVDWALTRSLTAPRAGWAISIPVPQTDTGVTLRSMDRATVLYELGHFIAGMD
                     DKAGEAERHYRAALEVNPRHAPSLAGLASLRIADAKYAEAMPLFERAI"
BASE COUNT          574 a          971 c          940 g          567 t
ORIGIN      
        1 cttcggaacg cccatcatca accagccgca agtcgccatc ctcggcgtcg gcggcgtgaa
       61 gaagcgcccg gtcgtcgtcg aaacaaaaga aggcgacgtg atcgccatcc gctcgatgtg
      121 catcctcgcg ctgaccttcg atcatcgcct cattgacggc gccgtcgccg atcaattcat
      181 ggcccgcctc cgctccttga tcgaagccgg ccagttcacg ctctgaagtg acgcggcggc
      241 ctgcgttttc gcaaggccgc cgcatcatca aacactacgg aagttttcct gttgccacga
      301 gaacgagctc cgcgaagccg aaatggccgc tgcgggtgag ctccttgatc ggccgccagc
      361 acggttgctg cgacagatac gaatggttgt cgccggtcat caaacccgcg atgacttcgg
      421 tcacgatccg cccgccgacc ggcccgaggg tgatcggagt gtcgtcatcc tcgaattgca
      481 actgaggttc ggcgaggatg tagaaccaga gcggtgcgtt gtccgcgaag ttcgggctga
      541 tgtcaatgag cttcggattg ttcggaaaat cctcttccgt cagtttgccg catttgagct
      601 ggtcgtcagg aatcggatcg atgcacatgt gtcgcgccac cgtctggcca gacgggaggc
      661 cgaacgtgag gccgcgcaga aggttccgct gtgccagcga tgggatgccg tcggtgatga
      721 acggcagatt gcgcagcgga tcgacgagcg aggtgtcgat cttgtacgcc ggctgcacgc
      781 gcgtcggacc ggtgtccgga gcgccgccgt gaatgtggaa gaagagcggc cactcgatcg
      841 cccacggagt cggaaactca tggaacccgc gaagatcggg aaagatcggg aagcggttcg
      901 gtagcgtcga gttgagccgg tattgcggac gaatcatgga atggccgaag cggtatgcgg
      961 cgaccgagaa ctcgatcggc atgaacgggg agtcgcgcca gtggaagaaa cgcagatccg
     1021 gcttctcgtc gtacggcgac ttgtccttcc cgtctttgcg cagatgcggc agaacgttgc
     1081 gaaccatctc ccatcccgcg atggtcggca gaaagtcgtg caggaccgcc cactgataat
     1141 gccagcggac gacgcgctgc acgtcggcaa acttcgcgct gccgccgagt agatcggcaa
     1201 tccggttgtg gaagcgaatg aagatcccct gcaactgcga gacgatgacg ttctcgtcgt
     1261 ttcgcggatc gccgatgatc gcgcgccgct cgccagcatc gcgggtgtcg cggggaagat
     1321 cgaacgtgtt cggatcgccc ggattgccgg tcagcagacg cgttccgtga agcagatgga
     1381 tgccgtccga ttcgtagagg tacggctgat catccggacc gcggccatag acggaatcga
     1441 ggtcgaaccg cggcgtgcgg aagtcgacca gtccatcggg atcgttcgtc ttctgcaggc
     1501 tgctcgcagg atcgaacgtg atgtcgtggt cgatgaactg tcccacgtac gtgtagcccg
     1561 cggagatccc gcggttctct tccggatcgg gagtcgcgtc tcccggatcg ggctccggat
     1621 ccgcgatcat ccgattcgca agagtgatga gatccgcacg ctcgtgatcg gcggacggca
     1681 aactccggaa catccggcca aagcggccct cgtactgctc cgaccgtcgc ggcacatcca
     1741 accctcgaac accaacgccg tgggcacgaa tgacgttggc caaactgaaa cttgcactct
     1801 ggtcagcagg cataatccct ccgtaataaa agagtgacgg acagtctgcc acgattttcg
     1861 ccgctccgga actttctgcc gtgctgcctc ttgttcttca ccgccgatga gtcgatggat
     1921 cgcactgctc ggtctcgtgc tcgctgtttc ggcctatgga caggccatct ctcccgttgt
     1981 gaaaaactcg cacgccgctg ccgcgacatc gcgtccgatc gacgcccgca ttcccttccc
     2041 tgccgcaaac gagacctggc tccgcgcgcg ctcgaagcac ttcgtcttca tcagcagcgc
     2101 ggatgagaac cgcactcgcg caatcgccgc ggagctcgag acactagccg cggcgctgac
     2161 gaaggtggac gccacattca gcacttcgtc gggcccggcg acgcgcgtga tcgttttcac
     2221 gaacccccgc gagagcaggc cgtacttcga catgctgcgg gatcggccca actccgacgt
     2281 cagcggagtt tttgtttccc agcgcgacgg cggaacgatg ctgatcaacg acggctcgac
     2341 ctggcgtggc ggcgaccggc cgcccctcca cgagctcgtc cacgatctgc tggaatcggg
     2401 cggcgcgcgc atcccgctct ggctcgacga agggctggcc gagtacttca gcaatgccac
     2461 catccgccgc ggctccgttt cggcgggcga gccgatgaga aatcacatcg ccacgcttcg
     2521 ccagcgaaga cgcattcctc ccgccgagct cttcgtcacc acccgtgagt ccgacacata
     2581 caaccttccg ccgaagcagg atgtctttta cgcggaaagc tgggccatcg tcgattggct
     2641 ggtccgttcc gcgtccagca gcggcgccga gttctacgga ttcgttcatg acgtcgcaaa
     2701 cggcatcgat gtcgagacgg cactgcagct gcgctatcac cgttcactgc gcgatgttga
     2761 ttgggcgctt acgcgttccc tgactgcgcc gcgcgcagga tgggcgatct cgattccggt
     2821 accgcaaacg gacaccggcg tgaccctgcg gtcgatggac cgggcgacag ttctctacga
     2881 gcttggtcac ttcatcgccg gaatggacga caaggccggc gaggccgaac gccactatcg
     2941 cgcggcgctc gaagtgaatc cgcggcatgc gccatcgctc gccggactcg cttctctccg
     3001 cattgccgac gcgaagtacg ccgaggcgat gccgctgttc gagcgcgcca tc
//
LOCUS       DATLVW010000078         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_292510, whole genome shotgun sequence.
ACCESSION   DATLVW010000078 DATLVW010000000
VERSION     DATLVW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292510"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..3248)
                     /locus_tag="VNN25_02585"
     CDS             complement(135..3248)
                     /locus_tag="VNN25_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH90441.1"
                     /translation="MTHILNPASYGFSPFAVPTLVTAALMLFFGAGVMVRRMSRVAGA
                     FFAISAAASVWLIAFTFLYCAKDAATALFWSRAAYLGVPFLAPAIYHFTAEMLRIAKE
                     RRVAIGAAWGMAVVFSALAVTGVLVDRVQHYWWGFYPRYSLVVSPPFLLFFFGYLIAS
                     LVEFVRAFPKSQGVEKKRIRLLIIAFGVAYLGCVDYLPKFGFAVYPFGYAALLGFVVL
                     VAIAFRKYDLVAITPSIAAREIIGTMADVLFVCDRDGRIEFANAAAERVLGYPGESLV
                     GRQFEELLVAGDDDTFVESLRRRSIRNMERRFHASDGRQIDLMMSLAPVLQHGEPAGV
                     VILGRDMREQKDAEREVRRAVTLLESTLDSTADGILVIGDGGRRVLTYNQRFVEMWRI
                     PAELLEARDDRGLIGCVLDQLVDPEHFLRTIETLYAQPEAESFDLLEFKDGRRFERYS
                     IGRAVEGIANVRVWSFRDVSARFAAEAALRESELRYRLLFEQNAAGVCVTEADGTIVD
                     CNLTFAMMLGYARSALIGSNAADLYVNSSDREDIVEMLRDSTTLNSVEVELEKQDGQS
                     MWALTNLTMGGERIHSTVVDISDRKRAEEQIEFHAYHDVLTNLPNRKLFTDRLTHSLS
                     RARRSGKPLAVMFVDLDHFKSINDTLGHEAGDELLLEMARRLRANIRDDDTVARLGGD
                     EFTVILAELRHPEDAVSVAEKLIQAIEQPLTIAGTSIEVSASIGIALFPDDGSDAESL
                     LRNADSAMYRAKESGRNTYHLCTDDMKRRAVERLSLETRLRRAITDGQLVLHYQPQIS
                     LTTGGAVGVEALVRWNDPERGMVHPSAFIPLAEESRLILPLGEWVLHTACAQMMSWRN
                     AGIDLPLMSVNISLRQFQQNDIVRTVRRVLADTGLDAGSLELEITETAAMQNAETTVD
                     VLQALRELGVSIAIDDFGTGYSSLNYLKRFPITAVKIDRAFIRDLATSEGDAAIVSAV
                     VGIARALKLRVIAEGVETEDQLSFLRRRNCDAAQGYLFSRPVSAATLPGRLIGHPLDA
                     PAFRMYV"
     gene            3333..>4198
                     /locus_tag="VNN25_02590"
     CDS             3333..>4198
                     /locus_tag="VNN25_02590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH90442.1"
                     /translation="MPSVVIAGAGVIGASIAWHLAARGVRDVVVLDRGSELGAGSTPR
                     ATGGFRAQFATPTDIALSLLSREKLRRFEAEIGVDSGYRPYGYLFIARSEAAMAQLRD
                     ANALQHACGLPEARLIGADEVRAINPAVKDDAIVGGTFCPTDGFIRAMKITNGYAAAA
                     QRDGVRFDFGVTVHRMRIEGDRIVALETSQGDVDADVFINAAGAWGSTLGIDVPVTPL
                     RRQVAATIEQDVLPESMPMTVWADDGFHFRVRDKRVLLLWPDTPPPGFDATFDGTTWL
                     PRVLDFTNARVP"
BASE COUNT          787 a         1328 c         1355 g          728 t
ORIGIN      
        1 ggcatgctcg cggccgtgcg caacgacgac gatccgcgag ggctttactg caccgcgtgt
       61 ttcactggga agtatcccgt gttgcacgaa gaggcggaag ccgcggcgtt tgcctagacg
      121 cgcgacgctc ggcgctacac gtacatccgg aaggctgggg catcgagcgg atgtccgatc
      181 agacgcccgg gcagcgtggc cgcggaaacc ggccggctga agaggtagcc ctgcgcggcg
      241 tcgcagttcc ggcggcgcag gaacgacaac tgatcctccg tctccacgcc ctcggcgatg
      301 acgcgaagtt tcaatgcgcg cgcgatgccg accactgcgg agacgatcgc ggcatcgcct
      361 tcgctcgtgg ccagatcgcg aatgaaggcg cgatcgatct tcacggccgt aatcgggaag
      421 cgcttcagat agttgagcga cgagtagccg gtgccgaagt cgtcgatcgc gatcgaaacg
      481 cccagctcgc gaagcgcctg cagcacatcc accgtcgttt ccgcgttctg catcgctgcg
      541 gtttccgtga tctccagttc gagtgaaccg gcatcgagac cggtgtcggc gagcacgcgc
      601 cgcacagtcc tcacgatgtc gttctgctgg aactgccgga gcgagatgtt caccgacatc
      661 agcggaagat cgatgccggc gttgcgccac gacatcattt gcgcgcaggc cgtgtgcagc
      721 acccactcgc cgagcggaag aatgagccgg ctctcctcgg cgagcggaat gaacgccgac
      781 ggatgcacca ttccgcgctc cggatcgttc caccgaacca gcgcttccac cccgaccgcc
      841 ccgccggtgg tgaggctgat ctgcggctga tagtgcagaa cgagctggcc atcggtgatc
      901 gcgcggcgca ggcgcgtctc gagcgacagg cgctccaccg cgcgacgttt catgtcgtcg
      961 gtgcagaggt gatacgtgtt ccggccggac tccttggccc ggtacatcgc gctgtcggcg
     1021 ttgcgcagca gcgactcggc gtcggaccca tcgtccggaa agagggcgat gccgatgctg
     1081 gccgagactt cgatcgaagt tcccgcgatc gtcagcggct gctcgatcgc ctggatcagt
     1141 ttctccgcca cactgaccgc atcctcggga tgacggagct ccgccaggat gaccgtgaac
     1201 tcgtcgccgc cgagtcgggc aacggtgtcg tcgtcgcgga tgttggcgcg aaggcgccgc
     1261 gccatttcga gcaacagctc gtcgccagcc tcgtgtccca gcgtgtcgtt gatcgacttg
     1321 aagtggtcga gatcgacgaa catcacggcc agcggcttcc cggaacggcg ggcgcgggaa
     1381 agactgtgcg tcagacggtc ggtgaatagt tttcggttcg gcagattcgt cagcacgtcg
     1441 tggtacgcgt gaaactcgat ctgctcctca gcgcgcttcc ggtcgctgat gtcgacgacg
     1501 gtagagtgga tccgttctcc ccccatcgtc agattcgtga gcgcccacat cgactgcccg
     1561 tcctgctttt cgagctcgac ctcgacgctg ttcagagtcg tcgagtcgcg gagcatttcc
     1621 acgatgtctt cgcggtccga tgagttgaca taaaggtcag cggcgttgct tccgatcagt
     1681 gccgaccgcg cgtatccgag catcatggcg aacgtcaggt tgcagtcgac gattgtccca
     1741 tccgcctcgg tcacgcaaac gccggccgcg ttctgctcga acaatagccg gtaccgcagc
     1801 tccgactccc gcagcgcggc ttcggcggcg aaccgtgcgg aaacgtcgcg gaagctccag
     1861 acacggacgt tggcaatgcc ctcgaccgca cgtccgatcg agtaccgctc gaagcgccgg
     1921 ccgtctttga actcgagcag gtcgaagctc tccgcctccg gctgcgcata caacgtttcg
     1981 atcgtgcgga ggaagtgctc gggatcgacg agttgatcga ggacacatcc gatgagaccg
     2041 cgatcgtccc tcgcttcgag cagttccgcg ggaatgcgcc acatctcgac gaagcgctgg
     2101 ttatacgtca gcacgcggcg gccgccgtcg ccgatgacga gaatgccgtc ggctgtcgaa
     2161 tcgagcgtcg attcgagaag cgtcaccgcc cgtctcacct cgcgctccgc atctttctgc
     2221 tcgcgcatat cgcggccgag gatgacgact ccggccggct cgccatgttg caggacggga
     2281 gcgagcgaca tcatcagatc gatctgacgg ccgtcgctgg cgtggaaccg gcgctccatg
     2341 ttccggatgc tgcggcggcg cagcgattcg acaaacgtgt cgtcatcgcc tgcaacgagc
     2401 aactcttcga actgacgccc gaccaggctc tccccgggat agccgaggac ccgctccgcc
     2461 gcggcattgg cgaactcgat gcggccgtcg cggtcgcaca cgaagagcac gtccgccatc
     2521 gtcccgatga tctcgcgcgc ggcgatggaa ggggtaatgg caacgaggtc gtactttcgg
     2581 aacgcgatcg cgacgagcac gacgaacccg agcagggccg cgtagccgaa cggatagaca
     2641 gcgaacccga acttcggcag gtagtcgacg cagccgaggt atgcgacgcc gaacgcgatg
     2701 atgaggagac gaatacgctt cttctcaacg ccttgcgact tcgggaacgc gcgaacgaat
     2761 tcgacgagcg aggcgatgag atatccgaag aagaaaagga ggaacggtgg tgacaccacc
     2821 aggctgtaac ggggatagaa accccaccag taatgctgca cgcgatcgac gagaactccg
     2881 gtcacggcca gagcggagaa gacgacggcc attccccacg ctgctccgat cgcgacgcgc
     2941 cgctctttcg cgatccggag catctccgcc gtgaagtgat agatggccgg ggcgaggaat
     3001 ggaacgccga ggtaggcggc gcgcgaccag aacaacgcgg tcgccgcgtc cttcgcgcag
     3061 tagagaaagg tgaaagcgat gagccacacc gaggccgccg cggagatggc gaagaacgcg
     3121 cccgccacgc gcgacatccg gcgcaccatg acgcccgcgc cgaagaaaag catcagcgcc
     3181 gccgtcacaa gcgttggtac agcgaacggc gaaaagccgt agctggccgg attcaggatg
     3241 tgtgtcacta aacctctcaa tctactgggg taacgctagt ttgaaggatg gaagcccgtt
     3301 tgtcgaaccg cacgggccat aatccacttt ccatgccgtc ggtcgtcatc gcaggagccg
     3361 gagtcatcgg tgcgagcatc gcctggcatc tagccgcccg cggcgtgcgc gatgtggtgg
     3421 tgctcgaccg cggcagcgag ctcggtgcgg gcagcacgcc acgcgccacg ggcgggtttc
     3481 gcgcgcaatt cgcgacgcca actgacattg cgttgtcgtt gttgtcgcgc gaaaaactgc
     3541 gtcgatttga ggccgagatc ggcgtcgatt cgggctaccg gccgtatggc tatctcttca
     3601 tcgcgcgcag cgaagcggca atggcgcagt tgcgcgacgc gaacgcgctg cagcatgcgt
     3661 gcggattgcc cgaggctcgg ttgatcggtg ccgatgaagt tcgcgcgatc aatcccgccg
     3721 tcaaggacga tgccatcgtc ggaggcacgt tctgtccgac cgacggcttc atccgcgcaa
     3781 tgaagatcac gaacggttac gccgcggcgg cgcagcgaga cggtgtccga ttcgattttg
     3841 gcgtcaccgt acatcgcatg cggatcgagg gcgatcgaat cgttgcgctg gagacatcgc
     3901 agggtgatgt cgacgccgac gttttcatca acgccgccgg tgcctggggt tcgacgctcg
     3961 gcatcgacgt gccggtaacg ccgctgcgcc ggcaggtggc tgcaacgatt gagcaggacg
     4021 ttctgcctga gtcgatgccg atgacggtct gggccgacga cggctttcac tttcgcgttc
     4081 gcgacaaacg cgtgctgctg ctctggcccg atacacctcc tccaggattc gatgcgacat
     4141 tcgacggcac gacctggctt ccgcgcgtgc tcgacttcac gaacgcgcgc gtgccgtg
//
LOCUS       DATLVW010000079         4323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_209946, whole genome shotgun sequence.
ACCESSION   DATLVW010000079 DATLVW010000000
VERSION     DATLVW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209946"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VNN25_02595"
     CDS             <1..360
                     /locus_tag="VNN25_02595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXH90443.1"
                     /translation="LRAVAQVLRDSCRVYDVPGRYGGEEFCVVLPETKTGNTTVVAER
                     IRERMESSSFNVGSDSVVVTASIGIAGIDSMEGEGNVSPSILIDRADRALYSAKHRGR
                     NRVELWDAAMQGAAMAH"
     gene            611..856
                     /locus_tag="VNN25_02600"
     CDS             611..856
                     /locus_tag="VNN25_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90444.1"
                     /translation="MLYAGPARTWWLFAAASALFVIIVTFAYFIPVLLRLPDAAKMPE
                     GQLTRMVRLWVLLNWFRFAILVAAWFAGLKAFAIALA"
     gene            complement(989..1411)
                     /locus_tag="VNN25_02605"
     CDS             complement(989..1411)
                     /locus_tag="VNN25_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH90445.1"
                     /translation="MDLVLKLREVRRLKGLSQKDVARLSGVGEKTISSFETGERVGSM
                     KLAQLKRLLAVYDMTEADFFGGGVEKKIAPWELDQDQFATVRMIEELQSLPKPSQRNL
                     LAKFQLMIETMSEVHEPRHRRPRYLRDDPEWQMMASRN"
     gene            complement(1433..2053)
                     /gene="lexA"
                     /locus_tag="VNN25_02610"
     CDS             complement(1433..2053)
                     /gene="lexA"
                     /locus_tag="VNN25_02610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXH90446.1"
                     /translation="MTYLTERQRDILQFIRDCQRERGVAPTHREICDHFGFSSYGTVY
                     KHLSLLQKKGLIRRDWNQKRGVELVENDDKPASATASGVLELPLFGYIAAGRPLDVEI
                     SNETITVPERLTSRGENYVLKVKGDSMIDDGIMDGDYVIIARRERAENGEMVVANVSG
                     EVTLKRFYHEGEKVRLQPANSMMHPIYASARDVAVQGVVVGLMRRF"
     gene            complement(2144..4123)
                     /locus_tag="VNN25_02615"
     CDS             complement(2144..4123)
                     /locus_tag="VNN25_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH90447.1"
                     /translation="MRIRLFALAAATLTACAHTAPPPTPPLAAAHATQTRAFIMGGRK
                     AGTETVVIDGPTRTVDFEFNDRGRGPKTHTVLTSGGNAITASLHTSGNDYFKVPVDET
                     YANGTWSNGAEKGNAPAGAPAFFVSMYGPPEETAVLARALLAAPEHRLALLPAGEASI
                     RKVGELTVSAAGRSQHVTCYEISGLGFTPSPIWLDDHNELFASASSWSSVIADGWDSV
                     LPQLLKAQDAWRDEAVSREAARLMRVPVGGGIVITNARLFDPVTLQTTPGTTIVIRGN
                     RIEAAGTGIAIPNGLEHIDAAGRTVIPGLWDMHTHNSADDGLLNISNGITSVRDLAND
                     TDFLLDLRKKIDSGAAIGPRIVLAGIIDGPGKYAGPTKVLVDTEAQAREAVENYAKLG
                     YEQIKIYSSVRPELVPFIARLAHEHGMRVSGHVPAFMRAEDAVRAGYDEIQHVNFLFL
                     NFWPDIQDTRTPVRFTAVAERAATLDLNSAEVKAFIELLHEHHTVIDPTVSVFEGMFV
                     SRKGTMSPSYATVADRLPPQVRRGFLTGGLPVPDGMDQRYRDSFRNLLAMVKALYDAH
                     VPIVAGTDAMAGYTLARELELYVEAGIPPAEVLRIATLGAAHVMKHDDQLGSIAPGKL
                     ADLDIVDGDPATNISDVRKVVTVIKDGKVFDPKAR"
BASE COUNT          758 a         1343 c         1357 g          865 t
ORIGIN      
        1 ctgcgcgcgg tggcccaggt gctccgcgat agttgccgtg tgtacgacgt tccgggccgc
       61 tacggcggcg aagagttctg cgtcgttctt ccggaaacga agaccggcaa cacgacagtc
      121 gtcgccgaac gaatccgtga acgaatggag tcgagctcct tcaacgtggg cagcgattcc
      181 gtcgtggtaa ctgcatcgat cggaatcgcc ggcatcgact cgatggaagg cgaaggaaac
      241 gtcagcccct cgatcctcat cgaccgcgcc gatcgcgcgc tctactcagc caagcaccgc
      301 ggccgcaacc gggtcgaact gtgggacgcg gccatgcagg gcgcggcgat ggctcactag
      361 cggagttggc caagaaaact catggcgttc catgacattg cgctctggct tctcgtcatc
      421 tcgctcggga ttcaaacagg cgccggcctg ttcgagacgc gcgtgctggt gccgttatgg
      481 gcttcctctc cgccgcagtc cgtgctggcg tacctcgcgg tcacgatccg cccggattcc
      541 ggccaccgcc tctggatctt tctgacgccg ctcaccggga tcatttcgct gctgaaatct
      601 cgtagcggcc ttgctgtacg cagggccagc gcgaacatgg tggctcttcg ccgccgcgtc
      661 ggctctcttc gtcatcatcg tgacgttcgc ctacttcatc ccggtgctat tacgcctccc
      721 ggatgcggcg aagatgccgg aggggcagct cacgagaatg gttcgattgt gggtactgct
      781 gaattggttc aggtttgcga ttctcgtagc cgcatggttt gctggactga aggcattcgc
      841 gatcgctctc gcgtagcaga actgatgcgg ctcggccggc aaactgcgcg ttagaagatt
      901 tcgcgggtgg aacttgagcg ggccggcaac ggtcggatca agtcacgcgg cgcgggtcgt
      961 tctgttcggc cgaggaggca cgcgcccttc agttccgcga agccatcatc tgccactcag
     1021 gatcatcgcg aaggtagcga gggcgcctat gccgcggctc gtgaacctcc gacattgtct
     1081 cgatcatcag ctggaacttc gccaggaggt tacgctgcga cggtttcggc agcgactgaa
     1141 gttcttcgat catgcgcacg gtggcgaact ggtcctgatc gagctcccat ggcgcgatct
     1201 tcttctccac acctccgcca aagaaatcgg cctcggtcat gtcgtagacc gcgagcagcc
     1261 gcttgagctg tgcgagtttc atcgagccga cgcgctcccc ggtctcgaac gagctgatgg
     1321 tcttttcgcc gacgccggag aggcgcgcca catccttctg gctcaggccc ttgaggcggc
     1381 ggacttcacg aagtttgagt acgagatcca tggttcttcc tcactttcgc gatcagaaac
     1441 ggcgcatcag accgacgact actccctgca ccgcgacgtc gcgggcggag gcgtaaatcg
     1501 gatgcatcat tgagttggca ggctgcaggc gaaccttttc gccctcgtgg tagaaacgct
     1561 tgagggtgac ttcgccgctg acgttggcga cgaccatctc gccgttctca gcgcgctcgc
     1621 ggcgggcgat gatgacgtaa tcgccgtcca tgatgccgtc gtcgatcatc gagtcgccct
     1681 tcaccttgag cacgtagttt tcgccgcggc tggtgaggcg ctccgggact gtgatggtct
     1741 cgttggatat ctcgacatcg agcgggcggc cggcagcgat atagccgaat aacggcagct
     1801 cgagcactcc ggacgcggtc gccgaggccg gcttgtcatc gttctcgaca agctcaacgc
     1861 ctcgtttctg gttccagtcg cgtcggatca gccccttctt ctggagcagg gagaggtgct
     1921 tgtagaccgt accgtacgat gaaaacccga agtgatcgca gatctcacgg tgcgtcggcg
     1981 ccaccccgcg ctcccgttgg caatcccgga tgaactgcag aatgtcgcgt tgtcgctcgg
     2041 tcagatacgt catgtccgcc tcgtgaaagg acaatatagg cggtaacaac gcgaaagtca
     2101 agcggaagtt agcgtaatgt aggcgtaaaa ggcgtaagtg cctctaccgg gccttcggat
     2161 cgaacacttt cccgtccttg atcacggtga cgaccttccg tacatcactg atgttcgtcg
     2221 ccggatcgcc gtcgacgata tcgagatcgg ccagcttgcc cggcgcaatc gagccgagct
     2281 ggtcgtcgtg cttcatcacg tgagcggctc ccagcgtggc gatgcgcaac acttcggcgg
     2341 gtgggatacc ggcttcgacg tacagctcga gctcgcgagc cagcgtatag ccggccatag
     2401 cgtcggttcc ggccacgatc ggcacatgtg cgtcgtagag cgcttttacc atcgccagga
     2461 ggttgcgaaa cgagtcgcgg taacgctggt ccatgccgtc gggtaccggt agcccgccgg
     2521 tgagaaatcc gcggcgcacc tgcggcggca gacggtcggc gaccgtcgcg tagctcggcg
     2581 acatcgtccc tttgcgcgaa acgaacatcc cttcgaagac cgagactgtc gggtcgatca
     2641 cggtgtgatg ctcgtgcaga agctcgatga acgctttgac ctccgcagaa ttcaaatcga
     2701 gggtcgcggc gcgctcggcg accgccgtga accgcaccgg cgtgcgcgtg tcctgaatgt
     2761 cgggccagaa gttgagaaag agaaaattga cgtgctggat ctcgtcgtag ccggcgcgaa
     2821 cggcatcctc cgcgcgcatg aacgccggca cgtgtccgct cacgcgcatc ccgtgctcat
     2881 gagcgaggcg ggcgatgaat ggtacgagct ccggccgaac cgaactgtag atcttgatct
     2941 gctcgtatcc gagcttcgcg taattctcga ccgcctcgcg cgcctgcgcc tcggtatcga
     3001 cgagcacttt cgtcggacct gcgtatttgc cgggaccgtc gatgatgcct gccaggacga
     3061 tgcgcgggcc gatcgcggcg ccactgtcaa tcttctttcg caggtcgagc aggaaatcgg
     3121 tgtcgttggc gagatcgcga accgaggtga tgccgttgga gatgttgaga aggccgtcat
     3181 cggcggagtt gtgagtgtgc atgtcccaca ggccaggtat gactgtgcga ccggccgcat
     3241 cgatgtgctc gaggccatta ggaatggcaa tgccggttcc ggcggcttcg atgcgattgc
     3301 cccggatcac gatcgtcgtt cccggcgtcg tctgcagcgt gaccggatcg aacagacgcg
     3361 cgttcgtgat gacgatgccg cctcccactg ggacgcgcat gaggcgcgcg gcctcacgcg
     3421 agaccgcctc gtcgcgccat gcatcctgcg ctttcagcaa ttgcggcagg acgctgtccc
     3481 agccatcggc gattaccgac gaccaggagg atgccgaggc aaacagctcg ttgtggtcat
     3541 cgagccagat tggcgacggc gtgaagccaa gcccgctgat ctcgtaacag gtaacgtgct
     3601 gcgagcgtcc ggctgctgac accgtgagct cgccaacctt gcggatcgat gcctctcccg
     3661 ccggtagcaa cgccaaacga tgctccggcg cggccagcaa cgcgcgcgca agcaccgcgg
     3721 tttcctcggg tgggccgtac atcgagacga agaacgccgg tgcgcctgcg ggtgcgtttc
     3781 ctttctccgc accgttcgac cacgtgccgt tcgcataagt ctcatcgacc ggcaccttga
     3841 aatagtcatt gccgctggtg tgcaacgacg ccgtaatcgc gttcccgccg ctcgtcagca
     3901 ccgtgtgcgt cttcgggccg cgccctcggt cgttgaactc gaagtcgacc gtgcgcgtcg
     3961 gtccgtcgat caccactgtc tctgtccccg ccttccggcc ccccatgatg aaggcgcgtg
     4021 tctgggttgc atgcgcggcg gccagtggcg gagtcggcgg aggagctgtg tgcgcgcatg
     4081 cggtcaacgt agcggcggcg agagcgaaga ggcggattcg catccgcaaa gtgtacgatc
     4141 tcggcgcatg ccgcgcgcgc ccgtcaccgt catcatccac accctcgatg aaaccgcctt
     4201 catcgaggac tgcctccgct cggtggcgtg ggcagacgag atctacttgc tcgattcctt
     4261 ttcgaccgat ggtacggtgg agctcgttcg cgagcgtttt ccggccgttc gcatcgaaca
     4321 gcg
//
LOCUS       DATLVW010000080         9828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_250842, whole genome shotgun sequence.
ACCESSION   DATLVW010000080 DATLVW010000000
VERSION     DATLVW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250842"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VNN25_02620"
     CDS             <1..678
                     /locus_tag="VNN25_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXH90448.1"
                     /translation="GKGFSVVAAEIRDLSERTASSTREIANLIRSVQEEVGNALRSMS
                     SGTKLVEDGVSLSHEAGKALNNILDSSTKASGMGKEIAAGTREQTAGSENITRAIDRL
                     QELLSQINGATTQQAQGSDHIMNAVESMREVTKYVRQAMTEQRSGSSMISASAEKMID
                     MIHEIFQVATNQAAESEKIVATMEQVRALAEGSGSYAAEMGESLTLLSDAIRGLDEEI
                     RRFRVRG"
     gene            complement(691..1761)
                     /gene="prfA"
                     /locus_tag="VNN25_02625"
     CDS             complement(691..1761)
                     /gene="prfA"
                     /locus_tag="VNN25_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXH90449.1"
                     /translation="MYEKLDEIEKRYEELQQKMADPAVAVDVAAFRDTMKAVSEIDDI
                     VAKYRQLKDVRKRLADAREMLKSDDDMRELAEMEIAELEFRAPELEKEIQILLQPKDP
                     NDDKNVFVEIRAGTGGDEASLFAAEIMRMYIRYAETRRWKVDMTDANYSSVNGIKDAT
                     LLIEGNKVYSRLKFESGVHRVQRVPQTETQGRIHTSAITVAVLPEVEDIEIQVNEKDL
                     RIDTFCSSGPGGQSVNTTYSAVRMTHLPTGVVVSCQDEKSQIKNRQKALKVLKARLYD
                     IERLKRESELSATRKGLIGSGDRSEKIRTYNFPQDRVTDHRIGLTVHNLPDIMNGNLD
                     VLLEPLLGHFQAEKLREAVEAR"
     gene            complement(1836..2855)
                     /locus_tag="VNN25_02630"
     CDS             complement(1836..2855)
                     /locus_tag="VNN25_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HXH90450.1"
                     /translation="MRAPKAWLGSARLISGTFGAVMNGLTAAMPAGTADRNVDMLIGG
                     QAVIEGVMMRSLTGYSVAVRQPNGDVAIKKDKLVSVTTKYPFLKFPVLRGSVVLIQSL
                     ILGIRALNYSASVATASEEGEKEMSSWAVASSMLMALVLGVGVFILAPLGITNLIRHY
                     FAPGMGNLAYNAIDGVLRALFFFVYILSISFMDEIKRVFQYHGAEHKTVYTFEASEDL
                     TVENARKKSTLHPRCGTSFLMFVMAISILVFSLVPSTVPFAVKFLARVVLIPLIAGLA
                     YEVIRFSARHLGNPVCRMLTRPGMWLQKITTKEPDDSQLQVAITALKEALMYDIVEET
                     QAAVA"
     gene            complement(2855..3082)
                     /gene="rpmE"
                     /locus_tag="VNN25_02635"
     CDS             complement(2855..3082)
                     /gene="rpmE"
                     /locus_tag="VNN25_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXH90451.1"
                     /translation="MKPEIHPKYQQVQVHCACGEQWMTGSTKKELRVEICSKCHPYFT
                     GKQKLLDTAGRIDRFQQRYAKKAGPKEAAAK"
     gene            complement(3169..3396)
                     /locus_tag="VNN25_02640"
     CDS             complement(3169..3396)
                     /locus_tag="VNN25_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH90452.1"
                     /translation="MHVTWQELRDVCRLLGCVQARIKGDHLIMTRAGLARPIVIKMDR
                     GIGEDIIRSNMRTLGIDRTEFERLLAIVRKR"
     gene            complement(3408..3740)
                     /locus_tag="VNN25_02645"
     CDS             complement(3408..3740)
                     /locus_tag="VNN25_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH90453.1"
                     /translation="MNETISLDVELRAFVRRDTDERWMAVCPSIDVASQGADADDAKR
                     CLDEAVQLWFESCIERGTLERALQESHFRPLHDAEVDRADVDTTTDDLLGEPFIVSVT
                     IPAYRATA"
     gene            complement(3766..4356)
                     /locus_tag="VNN25_02650"
     CDS             complement(3766..4356)
                     /locus_tag="VNN25_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HXH90454.1"
                     /translation="MSKLQFRFSLPKFVQALVFLSKNGVSDLTKLKAAKLFYFADKKH
                     MLKYGRPIIGDSYFALNLGPVPSQADDFFDEALAAHQAGPSTPEQEEFLQYLDVVADY
                     WPRYVANGSENFRVFSKSDLEVLGDVAKKYGRLHWKQLVDLSHEEPAYRLADKGRAPN
                     GRAPMTYETFFEGADSEMLKLAEVEQEHRDLVAAVA"
     gene            complement(4462..5466)
                     /locus_tag="VNN25_02655"
     CDS             complement(4462..5466)
                     /locus_tag="VNN25_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXH90455.1"
                     /translation="MPSHLFLLVAGLIGVALVFDYINGFHDAANSIATVVSTRVLSPG
                     QAVIWAAFFNFVAAFGFGTAVAKTIGNGMIDIHAVTFGVIFGGLIGAIVWDLITWYFG
                     LPTSSSHALIGGYAGAAVARYGFGAIIPSGWTKTLVFIVIAPLIGLVLGFLLTVAIMW
                     IFQRKSPKSVDKTFRRLQLVSAAAYSLGHGTNDAQKTMGIIAGVLFTAGYLKTFYIPI
                     WVILAAHSAIGLGTLSGGWRIIHTMGSKITKLQPVGGFAAETAGAITLFLASSFGIPV
                     STTHTITGAIVGVGATRRLSAVRWGVAGRIVWAWILTIPMAAIMGAVTYWIVTLFGAK
                     "
     gene            complement(5466..6083)
                     /locus_tag="VNN25_02660"
     CDS             complement(5466..6083)
                     /locus_tag="VNN25_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXH90456.1"
                     /translation="MRFRLLPREETFYADMIAMSDQLKVGARLLEEMFAPDPPIADKA
                     HEIKEVEHKCDFLTHAIIQRLNKTFVTPMDREDIHSLARTLDDVMDAIDNAASLVPLY
                     KIDRVRPGARELAKVISSSADEVRRAVIALERRDGVMACAVEINRLENEADRIHQKAV
                     GQLFDEERDPIVVMKWKEILDLLEEATDRCEDVANLLENVVVKHS"
     gene            complement(6124..9396)
                     /locus_tag="VNN25_02665"
     CDS             complement(6124..9396)
                     /locus_tag="VNN25_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH90457.1"
                     /translation="MSNQVLIVASEVAARAAAEVAASLSFAPVVAGTEEEALALLDRE
                     RFALIAVTAGDDFPHLRAEAERRQPAAPLLELPEVNGDNSALRRLMIQYLDRRVRPKP
                     LGSEERYRFLSSILESFTGTLELKEVLRRIVTITRQELGADRAWLLHPVNAQAEFAKV
                     RFAVSAPEYTVPNEDYASTLSLKQSSNLIQRLLDASGPIVVMENDPDLDREQAAIFQV
                     RSEIVQVLRPRDDEPWAFGLHQISSARVWTDEEISLFAEIGRYATLALNNTLLHERAV
                     REMAKVNAILDQIPESAAIYDAQGRLERMNAAAQREPVSLFANDPESRVRKSRHRYID
                     GSPLEADDLPSMRAIRGDVVKSDYLVHDARSGDDRVVNLKAAPIRDDRNHIIGSVVLS
                     RDVTDERLTAEREAWRRRRAECLANLGVDTVTVQPSFEELDDPARRVADAVGGTAMIY
                     LYHSPSSELRMVGMASVAPIANEVQRFREYLTRNPFHAGEGIAGTVFQIGRPLLYSEF
                     GGKAATDFARDSTEAGLVAAMNEQTLIATPIESYGDRIGALVIARSDQRRSFDAEDLE
                     FAQAVAERIGAASHIHRLTRISQEGHRAAEELARREVDARVRFEAVIETAPIGVAVFS
                     ADELRIELANARFVDFVQRLGKIAIDTPLVGLRVDEVIPSLEKPLKQVAESGEMRFDE
                     AFKADTRPTPTYINRIVSAVRGRFSGITQNLTVLIQDVTDQVNARREIEDLAQAMAER
                     SARLDSILSSMTDGLWVYDAGGQVVSVNEAAITMFGFGSRTEAAEHGSFAQLNLRYPD
                     ARPIPADDLPHARALGGMTVPDYLAMGRHFITGKDLDLSIAAAPIESNGIVGAVLVIR
                     DITALQELDRKKDEFLSVASHELRTPLTTIKGYTQLLAQTINDLPPEERMTYLNAVLG
                     EVDRMMGLISELLDVSRIETNRLQIDRQPVRWLEFLDRRANAFRVQNPARTIRFSAEI
                     AETTMPVDPDRMRQVVDNLISNAIKYSAEGTNIELVARIEENQMLTAVIDQGIGIPTD
                     EIPKLFERFHRARNVSSRYYGGLGLGLYIAKAIVEAHSGSIHVTSEEGQGATFTIRLP
                     LP"
     gene            9464..>9828
                     /locus_tag="VNN25_02670"
     CDS             9464..>9828
                     /locus_tag="VNN25_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HXH90458.1"
                     /translation="MPKRSFDELVQLMHTLRGPNGCPWDRKQTLATLKPFVIEESYEV
                     VDAIDRDDRAALAEELGDFLLQAVFIAELTSEEGSFDIYDAVTAIHDKLVRRHPHVFG
                     DVEANDAEEVLVNWEKLKN"
BASE COUNT         1876 a         3017 c         3056 g         1879 t
ORIGIN      
        1 ggcaagggct tctccgtcgt agccgccgag attcgcgatc tttccgagcg caccgcatcc
       61 tcgacccgcg agatcgccaa tcttatccgc tccgtgcaag aagaagtcgg caacgcgctc
      121 cgctcgatgt cttcaggcac caagctcgtc gaagacggcg tatcgctctc gcacgaggca
      181 ggcaaggcgc tgaacaacat cctcgattcg tcgacgaaag cgtcgggcat ggggaaggag
      241 atcgccgccg gaacgcgcga gcaaacggcc gggagcgaga acatcacgcg cgctatcgac
      301 cggttgcagg agcttctaag ccagatcaac ggcgccacca cccaacaggc gcagggctcc
      361 gaccacatca tgaacgccgt cgagtcgatg cgcgaggtga cgaagtacgt gcgccaggcc
      421 atgaccgaac agcgctccgg ctcctcgatg atctctgcct cggccgaaaa gatgatcgac
      481 atgattcacg agatcttcca ggtggccacc aatcaggctg ccgaaagcga gaagatcgtg
      541 gccacgatgg agcaggtgcg cgcgctcgcc gaaggcagcg ggagctatgc cgccgagatg
      601 ggcgaatcgc tcacgcttct ctctgacgcc atccgaggat tggacgaaga gatccgccgc
      661 ttccgcgtgc ggggctgatc gttcgtccgg tcagcgcgcc tcgaccgcct cgcgcagctt
      721 ctccgcctgg aagtggccga ggagtggctc gaggaggacg tcgagattgc cgttcatgat
      781 gtcggggagg ttgtggacgg tgagaccgat gcggtgatcg gtgacgcgat cctgcgggaa
      841 attgtaggtg cggatcttct ccgatcggtc gccgctaccg attaatcctt ttcgtgtcgc
      901 cgacagctcc gactcacgct tcagacgctc gatatcgtag agacgagcct tcaggacctt
      961 gagcgctttc tgccggttct tgatctgcga cttctcgtcc tgacacgaca ccacgactcc
     1021 ggtcggaagg tgcgtcatgc ggaccgccga gtaagtcgtg ttcaccgact ggccgccagg
     1081 cccggacgaa cagaaggtgt cgatgcgcag gtccttttcg ttgacctgaa tctcaatatc
     1141 ttcgacctcg ggaagaactg ccacggtgat ggccgaggtg tggatgcggc cttgcgtctc
     1201 agtctgggga acgcgctgca cgcggtgaac acccgactcg aatttcagtc tcgaatagac
     1261 cttgttgcct tcgatgagca gagtggcgtc tttgatcccg ttgaccgagg agtaattggc
     1321 gtcggtcatg tcgactttcc agcgacgcgt ctcggcgtag cggatgtaca tgcgcatgat
     1381 ctcggcggcg aacagcgacg cctcatcgcc gcccgttccg gcgcggatct cgacgaagac
     1441 gttcttgtcg tcgttcggat ccttcggttg cagcagaatc tggatctctt tctccaactc
     1501 cggcgctcgg aactcgagct cggcgatctc catctctgcg agctcacgca tgtcgtcatc
     1561 cgacttcagc atctcgcgcg cgtcggcgag acgcttgcgg acgtccttca gctggcggta
     1621 tttggcgacg atgtcgtcga tctccgacac agccttcatc gtgtcgcgga aggccgcgac
     1681 gtcgacggca acggccggat cggccatctt ctgttgcagc tcttcgtaac gcttttcgat
     1741 ttcgtcgagt ttctcgtaca tggtgcagtc ccgctgaaaa caaaaatggg aagacgcggc
     1801 atgcgccttc ccacggtgaa tgtgacgaac gtttgctaag caaccgcggc ttgagtttcc
     1861 tcgacgatgt cgtacatcag cgcttctttg agcgcggtga tcgcgacctg gagctgtgag
     1921 tcatccggct ccttcgtggt gatcttctgc agccacatgc cgggacgggt cagcatccgg
     1981 caaacgggat tgccgaggtg acgagcagaa aagcggatca cttcatatgc aagaccggcg
     2041 ataagcggaa tcaggaccac gcgcgccagg aacttcacgg cgaacggcac ggtcgaaggg
     2101 acgagcgaga agaccaggat cgagatggcc atcacgaaca tgagaaagct cgtgccgcag
     2161 cgcggatgga gcgtcgactt ctttctcgcg ttctcgacgg tcaggtcctc gctcgcttca
     2221 aaggtgtaga ccgtcttgtg ctcggcgccg tggtactgga agacgcgttt gatctcatcc
     2281 atgaacgaga tcgagaggat gtagacgaag aagaacagcg cgcgcagcac accatcgatc
     2341 gcgttgtagg cgaggtttcc catccccggc gcgaagtagt gacggatcag gttcgtgatc
     2401 ccgagcggcg cgaggatgaa tacgccgacg ccgagaacga gcgccatcag catcgagctc
     2461 gcgacggccc agctcgacat ctccttctca ccctcttcgc tcgccgtagc cacgctcgcg
     2521 gagtagttca gcgcgcggat gccgaggatg agcgactgga tgaggacgac cgagccgcgc
     2581 agtaccggga acttgagaaa cggatacttc gtcgtgaccg agacgagctt gtccttcttg
     2641 atcgcgacgt cgccgttcgg ctgacggacc gcgacggaat agccggtgag cgagcgcatc
     2701 atgacgccct cgatcacggc ctgaccgccg atcaacatgt cgacgttacg atccgccgtt
     2761 ccagccggca tggcagcggt gagcccgttc atcacggcgc caaaggtgcc gctgatcagt
     2821 ctcgccgagc caagccaggc cttcggggct cgcattattt tgccgccgct tctttcggtc
     2881 cggccttctt cgcgtagcgc tgctggaaac ggtcgatgcg gccggcggtg tcgagcagct
     2941 tctgcttgcc cgtgaagtaa ggatggcact tcgagcaaat ctcgacgcgc agctctttct
     3001 ttgtcgagcc agtcatccat tgttcgccgc aggcgcagtg cacctggacc tgctgatact
     3061 tcgggtgaat ctctggtttc attggattta cctctctgag gacctttccg agtcgtcctg
     3121 aaaacggcga gtatatccaa aaaattctgg gcggttccgg tgccggaatt agcgcttcct
     3181 gacgatggcc agcaggcgct cgaactccgt ccgatcgatt ccgagcgtac gcatgttgga
     3241 tcgaatgatg tcttcgccaa tgccccgatc catcttgatg acgattggcc tggccaggcc
     3301 ggcccgcgtc atgatgaggt ggtcaccttt gatgcgagct tgtacgcatc ccaacagacg
     3361 acagacgtcg cggagttcct gccaggtgac gtgcatcgac ccgactctta cgcggtggct
     3421 cggtacgccg gaatcgtcac actaacgatg aacggctcgc ctagaaggtc gtccgtggtc
     3481 gtatcgacgt ctgcccggtc gacttccgcg tcatgcaggg gacggaagtg tgattcctgc
     3541 agggcccgct ccagcgtccc tcgttcgata cagctctcga accagagctg aacagcctca
     3601 tcaaggcatc gttttgcatc gtctgcatcg gcaccctgcg acgcaacgtc gatcgaagga
     3661 cagacagcca tccagcgctc atccgtatcg cgccgcacga acgcgcgcaa ctccacatcg
     3721 agggagatcg tttcgttcac ggtatgagga taaccctact tcgcgtcatg ctaccgctgc
     3781 aaccagatcg cggtgctcct gctctacctc ggcgagcttc agcatctcgc tgtccgcgcc
     3841 ctcgaagaat gtctcgtacg tcatcggagc gcggccattc ggtgctctgc ccttgtcggc
     3901 tagccgatac gccggctcct cgtgcgacag gtcgacgagc tgcttccagt gcagtcttcc
     3961 gtacttcttt gccacgtcgc cgagcacttc caggtcggac ttcgagaaaa cgcggaagtt
     4021 ctcgctgcca tttgccacgt agcgaggcca gtagtccgca acgacgtcga gatactgcag
     4081 aaactcctcc tgctcgggag tggaagggcc ggcctgatga gcggccaatg cctcgtcgaa
     4141 gaagtcgtct gcctgcgaag gaaccggacc cagattgagg gcaaagtacg aatctccgat
     4201 gatcggccgg ccgtacttga gcatgtgttt tttgtccgcg aagtaaaaca gctttgcagc
     4261 cttcagtttc gtgagatccg aaacgccgtt cttcgaaagg aagacaagcg cctgcacaaa
     4321 cttggggagg ctgaaccgaa actgaagttt gctcatctca tgaattcttc ggtagctacg
     4381 actatacgcc cgcgggctca agcagcgaga agcacaatgg cacggacgca aatttccgcc
     4441 cccgttagcg cttcgtcaac cctacttcgc gccgaacagc gtcacgatcc agtacgtcac
     4501 cgcccccatg atcgcggcca taggaatcgt caggatccac gcccagacga ttctccctgc
     4561 caccccccaa cgcacggcgg aaaggcggcg tgttgcgccg actccgacga tggcaccggt
     4621 gatcgtgtgc gtggtcgaga ccgggatgcc gaaggagctg gccaggaaga gcgtgatcgc
     4681 gccggcggtc tcggcggcga agccacccac cggctgcagc tttgtgatct tcgagcccat
     4741 cgtatgaatg atccgccagc cgcccgacag cgtgcccagg ccgatcgccg agtgcgcggc
     4801 gagaatcacc cagatcggga tgtaaaacgt cttgaggtac ccggcagtga agaggacgcc
     4861 agcgatgatg cccatcgtct tctgcgcgtc gttcgtgccg tgtccgaggg agtaggcggc
     4921 ggcggaaacg agctgcaggc gccgaaacgt cttgtcaacc gacttcggtg acttgcgctg
     4981 gaagatccac atgatcgcca ccgtgagcag gaatccgagc acgaggccga tcagcggagc
     5041 gatgacgata aagacgagtg tcttcgtcca gcccgaaggg atgatggcgc cgaagccgta
     5101 gcgcgccact gccgctcccg cgtaaccgcc gatgagcgca tgcgacgagc tcgttggcaa
     5161 cccgaagtac caggtgatca ggtcccagac gatcgcgccg atcaggccac cgaaaatcac
     5221 gccgaacgtt acggcgtgga tgtcgatcat cccgttgcca atagtcttgg ccaccgccgt
     5281 tccgaagccg aacgcggcta cgaagttgaa gaaagccgcc cagatcacgg cctgacccgg
     5341 cgagagaacc cgcgtggaga cgaccgtcgc gatcgagttc gccgcatcgt gaaagccgtt
     5401 gatgtagtcg aacaccaacg ccacgccgat caggccggcg accagcagaa agagatggct
     5461 cggcatcagc tgtgtttcac gaccacgttc tcgagcaggt ttgcgacatc ctcgcagcga
     5521 tccgtggcct cttcgagaag gtcgaggatc tccttccact tcatgaccac gatcggatcg
     5581 cgctcttcgt cgaaaagctg tccgacagcc ttctggtgga tgcggtctgc ttcgttctcc
     5641 agccggttga tctcgaccgc gcatgccatg acgccgtcac gccgttcgag cgcgatgacg
     5701 gcgcgccgga cttcatcggc cgatgacgaa atcacctttg ccagctcgcg cgcgcccggg
     5761 cggacgcgat cgatcttgta cagcggcacc aggctcgcgg cgttgtcgat ggcgtccatc
     5821 acgtcatcga gggtgcgggc gagcgagtgg atgtcctcgc ggtccatcgg cgtgacgaaa
     5881 gtcttgttga ggcgctggat gatcgcgtgg gtcaggaaat cacacttgtg ctcgacttcc
     5941 ttgatctcgt gagccttgtc ggcgatcgga ggatcgggcg caaacatttc ttcgagcaga
     6001 cgcgcgccca ccttgagctg atcggacatt gcgatcatgt cggcgtaaaa cgtttcttcc
     6061 cgggggagca ggcggaatcg caagggggga acctccgtcg aaaacgggcg gattgtatcg
     6121 gttttacggc aacggcagcc ggatcgtgaa cgtcgcgccc tgcccttcct cgctggtgac
     6181 atgaatcgag ccgctgtgcg cttcgacgat ggccttggcg atgtagaggc cgaggccgag
     6241 accgccgtag tagcggctcg agacgtttcg tgcgcggtgg aagcgctcga acagcttcgg
     6301 gatctcgtcc gtcggaatgc cgatgccctg atcgatcacc gcggtcagca tctgattctc
     6361 ctcgatccgc gcgacgagct cgatgttggt tccttccgcc gaatacttga tcgcgttgga
     6421 gatgaggttg tcgacgacct gccgcatccg gtctgggtca accggcatcg tcgtctccgc
     6481 gatctcggcg gagaagcgga tcgtgcgtgc aggattctgc acacggaacg cgttcgcgcg
     6541 gcgatcgaga aactcgagcc agcgcaccgg ctgccggtcg atctgcagac ggttcgtctc
     6601 gatgcgggag acgtcgagca gctcggagat cagccccatc atgcggtcga cttcgccgag
     6661 gacggcgttg aggtaggtca tccgctcctc gggcgggaga tcgttgatcg tctgcgcgag
     6721 cagttgcgtg taacccttga tcgtcgtcag cggcgtccgt aactcgtgag acgcgaccga
     6781 gaggaattcg tccttcttgc ggtcgagctc ctgcaatgcc gtgatgtcgc gaatcacgag
     6841 aacggcgccg acgatgccgt tgctctcgat cggcgcagcg gcgatcgaaa ggtcgagatc
     6901 cttgcccgtg atgaagtgcc ggcccatggc caggtagtcg gggacggtca tacctcccag
     6961 cgcgcgagcg tggggaaggt cgtccgccgg aatgggccgg gcatcgggat agcgaaggtt
     7021 caactgcgca aacgatccat gctctgccgc ctcggtgcgc gagccgaagc cgaacatcgt
     7081 gatggcagcc tcattgacgg agacgacctg ccctccggcg tcgtaaaccc agagaccgtc
     7141 cgtcatcgag ctgaggatcg agtcgagtcg cgccgatcgt tcggccatcg cctgcgccag
     7201 atcctcgatc tcgcggcgcg cgttgacctg atcagtgacg tcctggatca gcacggtcag
     7261 gttctgggtg atgcccgaga aacgtccgcg cacagcggac acgatgcggt tgatgtacgt
     7321 cggcgtcggc cgcgtgtcgg ccttgaacgc ttcatcgaag cgcatctcgc ccgactcggc
     7381 gacctgcttg agcggcttct ccagcgacgg gatgacttcg tcgacgcgca ggccgaccag
     7441 cggcgtatcg atggcgatct tgccgagtcg ctgcacgaaa tcgacgaagc gggcgttggc
     7501 cagctcgatg cgcagctcgt ccgcggagaa gacggcgacc ccgatcggcg cggtctcgat
     7561 cacagcctcg aaacgaacgc gcgcatcgac ttcgcgtcgg gccagttctt cagcggcgcg
     7621 atggccttcc tgggagatgc gcgtcagccg gtggatgtgg ctggccgcgc cgatccgctc
     7681 cgccacggcc tgcgcgaact cgagatcctc ggcgtcgaaa ctgcgccgct gatccgagcg
     7741 cgcgatcacc agagcgccga tacgatcgcc gtacgattcg atcggcgtag cgatcagcgt
     7801 ttgttcgttc atcgccgcca cgaggccggc ctcggtcgaa tcgcgcgcaa aatcggttgc
     7861 cgccttcccg ccgaactccg agtagagcag cggccgtcca atctggaaaa ccgttcctgc
     7921 gatgccttcg cccgcgtgga aggggttgcg cgtcaggtat tcgcggaacc gctgcacttc
     7981 attggcgatg ggagcgacgg acgccattcc gaccatgcgc aactcgctcg acggcgagtg
     8041 atagaggtag atcatggccg tcccgccgac ggcatcggcg acgcggcgcg ccggatcgtc
     8101 gagctcttcg aatgacggct gtaccgtcac ggtgtcgacg ccgaggttgg cgagacattc
     8161 agcgcggcgg cggcgccatg cttcgcgctc ggccgtcaga cgctcgtccg taacatcgcg
     8221 cgacaacacc acggagccaa tgatgtgatt gcggtcgtcg cgaatcggcg ccgctttcag
     8281 gttgacaacc cggtcgtcgc cactgcgcgc atcgtggacg agatagtcgg atttgacgac
     8341 gtcgccgcgt atcgcacgca tcgacggaag atcgtccgct tcgagcgggc tgccgtcgat
     8401 gtagcgatgc cggctcttgc gaacgcggct ttcgggatcg ttggcgaaga gcgaaacggg
     8461 ctcgcgctgc gctgcggcgt tcatccgctc caaccgcccc tgcgcgtcgt aaatcgcggc
     8521 agactcgggg atttgatcga ggatggcgtt gaccttggcc atctcgcgga cggcgcgctc
     8581 gtgcagcagc gtgttgttga gcgcgagcgt ggcgtagcgg ccgatctcgg cgaagagcga
     8641 gatctcttcg tccgtccaca cacgcgcgga cgaaatctga tgaagcccga acgcccacgg
     8701 ctcgtcgtcc cgcgggcgga gcacctgcac gatctcggag cgcacctgga aaatcgcggc
     8761 ctgctcgcga tcgagatcgg gatcattttc catgaccacg atcggtccgc tggcgtcgag
     8821 gaggcgctgg atcagattcg atgactgctt caacgaaagt gtggatgcgt agtcttcatt
     8881 cggaaccgtg tattcaggcg cgctgacggc gaagcggact ttcgcgaact ccgcctgcgc
     8941 gtttaccgga tggagcagcc acgcccgatc ggcgccgagc tcctggcgcg tgattgtgac
     9001 gatgcgccgc agcacttcct tcagttcgag cgtgccggtg aaggactcga ggatgctgga
     9061 gagaaaccgg tagcgttctt cagacccgag gggtttcggc cggacacgcc ggtcgagata
     9121 ctggatcatc agccgccgca gcgcgctgtt gtctccattc acctcgggta actcgagcag
     9181 cggtgcggcc ggctgcctcc gctccgcttc cgcgcgcaaa tgcgggaaat cgtcgccggc
     9241 ggtcacggca atcagcgcga atcgctcgcg gtcgagaagg gcaagcgctt cctcctcggt
     9301 acccgccaca accggcgcga agctgagcga tgccgccacc tcagcggctg ctcttgcggc
     9361 cacttcggag gccacgatca gaacctgatt cgacatgcga gatggcgctg aagaaatcga
     9421 gcaacgatgc taccaccaag aatcgcacgc atagaatgcg ttcatgccga agcgctcttt
     9481 cgatgagctc gtgcagctca tgcatacact ccgcggcccg aatggttgtc cgtgggaccg
     9541 caagcagacg ttggccacct taaagccgtt tgtgatcgag gagtcgtacg aagtcgtcga
     9601 tgccatcgac cgcgacgatc gtgctgcgct agccgaggag ctcggcgatt tcctgctcca
     9661 agcggtgttc atcgccgagc tgacgagcga agaaggatcg ttcgacatct acgacgcggt
     9721 cacggcgatt cacgacaagc tcgttcgacg gcacccgcat gtgttcggcg acgtcgaagc
     9781 caacgacgcc gaggaggttc tcgtcaactg ggagaagctc aagaacga
//
LOCUS       DATLVW010000081         6713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_540532, whole genome shotgun sequence.
ACCESSION   DATLVW010000081 DATLVW010000000
VERSION     DATLVW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540532"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VNN25_02675"
     CDS             <1..294
                     /locus_tag="VNN25_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH90459.1"
                     /translation="VVLAGCRGASNEDPRAFIRTVADAFLIAGVPTVIATSYDLDDAE
                     APPTMRLLHTFLRNGDDAAEALRKTTLIELRSARGVPLSIRFQAVGGASALIK"
     gene            524..2545
                     /locus_tag="VNN25_02680"
     CDS             524..2545
                     /locus_tag="VNN25_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH90460.1"
                     /translation="MAQKKAVALVERKACERVMFGVGRARRFTQDSPVLPDVWIKFAE
                     DLDVKRELLLTPFAEARPGIVRRVVVDRLGEDPMTDGEPREVIYNQSTALATVSFAEL
                     VRVILPMTNWWSNMLWQRVDKKRVPINPGVLLQDTKNRQAVARALEDPERRASQLGVS
                     ADLLWVFRIVGFLAVARKEHRRRTESEFEPVLPDISTPGESSEPDAPPYDWSREVEAV
                     AELLEGVEPHEDVMSRIWTVNLNRQAATAIVRSSVSVKADAARRLFELSCKDLVWAVV
                     DSGVDATHPAFRRRKEADINGRQPIYEEPFELVNGKWMNRTRIVATYDFRLIRLLLNP
                     ARLNPPSPKLSQRVRDILTGDSKESHDIREELKTLTSRLLSGSEIDWSLLEPLLAVPH
                     TSAYTDEVLPSGETGHVPRNDHGTHCAGILAADWRAEEPGREKQPTMQGICPDLGLYD
                     LRVFDDAGLGDEFSIIAALQYIRFKNAHKDYFVIHGANLSMSIRHDVMNFACGRTPVC
                     EEAERLVNNGVVVVAAAGNEGYVRYETATGPSEDYRSISITDPGNADLVITVGATHRF
                     KPHTYGVSYFSSRGPTGDGRYKPDLVAPGEKIESTVPNENVSKKDGTSMATPHVSGAA
                     ALLMARYEELIGRPTRIKQILTSTTTDLGRERYFQGAGMLDILRALQSV"
     gene            2562..3698
                     /locus_tag="VNN25_02685"
     CDS             2562..3698
                     /locus_tag="VNN25_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90461.1"
                     /translation="MPLKLEALPAQHGDSLLLHYGPENDPQIIIIDGGPKGVYASSLR
                     PRLAELKDERSPDDPMPVRLVMVSHIDEDHILGILNLCDELSDSSGQRLIAVQELWHN
                     AFSDIVGDEDDLYLKSASGAAKVSSTGDVLVQLDVHPRTEAIIASVPQGKRLRETAKK
                     LGWILNDERPLMMAGTGNDTIDAGGELKLTILGPSRERLIALQKDWMKKVKEAQKKAE
                     ESGAQTVEYKDTSPPNLASMIVLATDGKRRILLTGDGRGDYILEGIENAGLMEDDKFP
                     VDVLKVPHHGSIRDLDTDFFERIPANDYIISANGRFSNPDIPTLEMIVAVRGNEPYNI
                     HLTYPVDQFNAAYDRQRLQELIDESQHKSYKIIPRDPSATSIVV"
     gene            complement(3789..4337)
                     /locus_tag="VNN25_02690"
     CDS             complement(3789..4337)
                     /locus_tag="VNN25_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90462.1"
                     /translation="MDEDTQAIVPLERSAPSETDAAQEVAVHPPEELAAGVAKVLDLI
                     AERLELEIPHPSTARQVRGARTVSREFVLSLMAAADRRPDLSFFARFDSAEARSVLES
                     EDAYRLTAERTAMFLSSLKYTIEARWARVVADAMLAFTMASIFAKDPMNADLAAEVEN
                     MRRHLGRKGVRKKKAKKGEPPE"
     gene            complement(4787..4969)
                     /locus_tag="VNN25_02695"
     CDS             complement(4787..4969)
                     /locus_tag="VNN25_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90463.1"
                     /translation="MRIVDDCSWKRVLEIWPAPLPGLADAAIVAVAAANRYEAVATFD
                     RKLSNRLEAFGLAAYW"
     gene            complement(5146..5805)
                     /locus_tag="VNN25_02700"
     CDS             complement(5146..5805)
                     /locus_tag="VNN25_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90464.1"
                     /translation="MESFEDRLRRFVKGADREFALTIFMMVRNALLIEVEDAAKKHLL
                     TLTLLGTHAAMQMVCEKVFGLKGIDATEFYLEQFVDGTAPDRKYSAIVAELHEQRNVM
                     AHQGFSSTQHVIAYDWGLSGGFEWKGNTLHLNPDIYIADFIAGAARYSKVATTYVTDF
                     EMAKRKYAFIRDWLDLPKGDSLDDQLKNVKNLPDLPALRAAEPPLQDVIVTKYGLYAR
                     R"
     gene            complement(5918..6337)
                     /locus_tag="VNN25_02705"
     CDS             complement(5918..6337)
                     /locus_tag="VNN25_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90465.1"
                     /translation="MKKTWAGWLSLGAGVFWQATGTAHHLEYLKKIIVDRLDVAAIGS
                     FVVQSPAIMVIALSCGWLIYVKQHSSHEDAVQNPEMVHCVPSPTRTIPPAREANPHPD
                     FEMPQHPQIAHNCDEIEVTERIILERKVVYRAAKSIR"
     gene            complement(6384..>6713)
                     /locus_tag="VNN25_02710"
     CDS             complement(6384..>6713)
                     /locus_tag="VNN25_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HXH90466.1"
                     /translation="GHVVLHTKELIARALHPFKPSLNRRSEHAIFRDTEWQGDAFAAA
                     FIAPLQAVLQLHDEIGYLSPTEIAQTFGLSKQAAAIRLDNVLDLVPRRRLRIVGRDPR
                     RQLADWS"
BASE COUNT         1387 a         2065 c         2018 g         1243 t
ORIGIN      
        1 gtggttctag ccggttgccg cggcgcttca aatgaagatc ctcgcgcatt cattcgaacg
       61 gtggcggatg cattcctcat tgccggcgtg ccgacagtca ttgcaacgtc gtacgatctc
      121 gatgacgcgg aagctccccc aacgatgcgc cttctgcata catttctccg caacggcgat
      181 gacgcggcgg aggcgcttcg caaaacgact ctgatcgaac ttcggtcagc gcgtggtgtg
      241 ccgctctcaa tccggttcca ggcagtcggc ggcgctagtg cgttgattaa gtaagggcgg
      301 cagaggcctg agctacgccg tcggacgtgc cagtcagcgc gagccgatgt cacgaaggca
      361 ccattcaccg ccgtgagtga tcgactctgg ggtcacaccg tcggttttcg aaagtctctt
      421 cgaacgcggg gcataactcg cacccgtcca cgaacctgga taggctcacg cggatgggta
      481 gggaaaacac tccttgcagc accacctact acaggggggt gacatggcgc aaaagaaagc
      541 tgtggcattg gtcgagagga aggcgtgcga gcgcgtcatg ttcggcgtgg gacgcgcgcg
      601 gcggttcaca caggattcgc ccgttcttcc cgatgtctgg atcaaattcg cggaagacct
      661 cgatgtgaaa cgcgagctgc tcctaacgcc gttcgccgag gcgagaccgg ggatcgtacg
      721 gcgcgtcgta gtggaccggc tcggtgagga tcccatgacg gacggcgagc cgcgcgaagt
      781 gatctacaac caatccaccg ctttggccac ggtctcgttt gccgagctgg tgcgcgtcat
      841 cctgccgatg accaactggt ggtcgaacat gctgtggcag cgcgtggaca agaagcgcgt
      901 ccccatcaat cccggcgtgc tactgcagga tacgaagaat cggcaagcgg tagcccgggc
      961 tctcgaggat ccggaacggc gcgcgtccca actcggtgtg tcggccgacc tgctgtgggt
     1021 cttccgcatc gtcggtttcc tcgcggtcgc gcgcaaagag caccgccgcc gcacggagag
     1081 tgagttcgaa ccggtgctac cggacatcag cacgcccggc gaatccagcg agccggacgc
     1141 gccgccctac gactggagcc gcgaagtcga ggcagtcgcg gaattgctgg agggagtcga
     1201 gccgcacgaa gacgtgatgt cccgcatctg gacggtgaac ctcaatcgcc aggcagcgac
     1261 ggcgatcgtg cgttcctccg tatcggtgaa ggcggacgcg gcgcggcggc tcttcgagct
     1321 gagctgcaag gatctcgtgt gggccgtcgt cgacagcggc gtcgacgcga cgcatcccgc
     1381 attccggcga cgcaaggaag cggatatcaa cggccgtcag cctatttatg aggagccgtt
     1441 cgagctcgtg aacgggaagt ggatgaatcg cacgcgcatc gtcgcgacat acgactttcg
     1501 cctcatccgg ctactactca atcccgcgcg tctcaacccg ccttcgccga agttgtcgca
     1561 acgcgtgcgc gacattttga ccggtgactc aaaagagtcg cacgatatcc gcgaggagct
     1621 gaagactctc acgtcgcggc tgctctccgg cagcgagatc gactggtcgc tgctggagcc
     1681 gctgctcgcg gttccgcaca ccagcgccta caccgacgag gtactcccga gcggcgaaac
     1741 aggacatgtt ccgcgcaacg accacggcac gcactgcgcc gggatccttg ccgcggactg
     1801 gcgcgcggag gagcccggcc gcgagaagca acccacgatg cagggcatct gtcccgacct
     1861 cggtctctac gatctgcgtg tcttcgacga cgccggcctt ggggacgagt tctcgatcat
     1921 cgcggcactc cagtacatcc gcttcaagaa cgcgcacaag gactacttcg tcattcatgg
     1981 tgcgaacctc tccatgtcca tccgccacga cgtgatgaac ttcgcctgcg gacgcacgcc
     2041 tgtgtgcgag gaggcggagc ggctcgtgaa taacggagtg gtcgtggtcg ccgcggcggg
     2101 caacgagggc tacgtgcgct acgagactgc cactggtccg tccgaggact atcggagcat
     2161 ctccattacc gacccgggca acgcggatct cgtcatcacc gtaggcgcga cgcaccgctt
     2221 caaaccgcac acgtatggcg tcagctactt ctccagtcgc ggtccgacag gcgacggacg
     2281 ctataaaccg gacctcgtcg cgccaggcga gaagatcgag tcgacggtgc cgaacgaaaa
     2341 cgtgtcgaaa aaggatggaa cgagcatggc cacgccgcat gtcagcggcg ccgcggcctt
     2401 gctcatggcc cgttacgagg agctgatcgg aaggccgact cgcatcaagc agatccttac
     2461 ctcgacgacg acggaccttg ggcgcgaacg atatttccag ggtgcgggga tgctggacat
     2521 cctgcgcgca cttcaatcag tgtgaacgaa agaggattgc catgccactc aagctcgaag
     2581 ctcttcccgc gcagcacggt gactcgcttc tcctgcatta cggaccagag aacgatccgc
     2641 aaatcatcat catcgacggt ggcccgaaag gcgtctatgc gtcctcactt cgtccgaggc
     2701 tcgcggaatt aaaggacgag cgatctccgg acgatccgat gcctgtccga ctggtgatgg
     2761 tcagccacat cgacgaagac cacatcctcg ggatcctcaa cctatgcgac gagctttccg
     2821 actcgtcggg ccagcgtctc atcgccgtgc aggagttgtg gcacaacgcc ttcagcgaca
     2881 tcgtcggcga cgaggatgac ctatacctca agagcgcgtc cggtgccgcg aaagtctcgt
     2941 cgaccggcga cgtgctcgtg cagctcgacg tgcatccccg caccgaggca atcatcgcca
     3001 gcgttccgca gggcaagcgc ctgcgagaga ccgcgaagaa gctggggtgg atcctcaacg
     3061 acgagagacc gctgatgatg gccggcaccg ggaacgacac catcgatgcc gggggcgagt
     3121 tgaagctcac catcctcggc ccgtccaggg agcgcctcat cgcgctccag aaggactgga
     3181 tgaagaaggt gaaagaagca cagaagaagg ccgaggagtc gggcgcgcag accgtcgagt
     3241 acaaggacac gtctccgccg aacctggcca gcatgatcgt actggccacg gacggcaagc
     3301 gcagaatcct tctcactggc gacggacgtg gcgactacat cctggaaggg atcgaaaacg
     3361 ccggcctgat ggaagacgac aagttcccgg tagacgtcct caaggtcccg caccacggca
     3421 gcatcaggga cctcgacacg gacttcttcg agcgcatccc ggccaatgat tacatcatct
     3481 cagccaacgg ccggttcagc aatccggata tcccgacgct ggagatgatc gtcgcagtgc
     3541 gcggcaacga gccgtacaac atccacctga cgtacccggt cgatcagttc aacgcagcct
     3601 acgaccgcca gaggcttcag gaactgatcg atgaatcgca gcacaagagt tacaagatca
     3661 tcccaaggga tccgagcgcg acgagcatcg tggtgtgaaa cctgaacgcc tagtcgcggc
     3721 gcttactttg gcggggccga cgggactcga acccgcgccg cccagcgtga caaccccgcg
     3781 ctctactctc actccggagg ctcgcccttc ttcgccttct tcttccgcac gcctttgcgg
     3841 cccaggtgcc gccgcatgtt ttcgacctcg gcggccaagt ccgcgttcat cggatccttg
     3901 gcgaagatgc ttgccatcgt gaacgcgagc atcgcgtcgg cgacgactct ggcccagcgg
     3961 gcttcgatcg tgtatttcag gctggaaagg aacatggcgg tgcgctcggc cgtgagccgg
     4021 taagcgtctt cggattccag cacggaccgc gcctcggcac tgtcgaatcg tgcgaaaaag
     4081 gaaaggtctg gacggcggtc cgccgcggcc atcagcgaca gcacgaactc ccgcgagacg
     4141 gtccgcgcac ctcgcacctg gcgtgccgtc gacgggtggg gaatctcgag ctccaggcgt
     4201 tccgcgatca ggtccagcac cttcgcaacc cccgccgcca gttcctcggg aggatggacg
     4261 gccacctcct gcgcggcgtc cgtttcggac ggcgcactcc tttccagcgg cacgatcgcc
     4321 tgcgtgtctt cgtccatagg tacctcctcg attacctctg acgaattgca gtgcgaaagg
     4381 ttacgcagca gaggcgggcc aacaatggcg tcgcgaatgt cggacggcga tacgttcgcg
     4441 atgacgcgga tcacctcgcg agacaatttc gtcgaattga atatctgacg ctcagacgtt
     4501 cgatacaacg cggaagtcat tcaaaagctc gccgctggtc atgaatatct gagcgtcaga
     4561 cattcgcaac gtccaaggac ccgcgaatgt ctaactgcca gacgttcgca atgacgcagt
     4621 agcttcgaat gtctgacggc cagacgttca cgatgacgcg gtcgcctcgc gagacaattt
     4681 cgtagaatcg aatgtctgac gctcggacgt tcgatgcaac gtggaagtca tccgcagcga
     4741 gtcatgaacg tctgagcgcc ggacactcgc agagatttac tgcgagctac caatatgcgg
     4801 ccaggccgaa cgcttcgagg cggttggata gcttccggtc gaaggtcgct accgcttcgt
     4861 atcggttcgc ggcggcaacg gcgacgatcg ccgcatccgc gaggcccggg aggggtgcgg
     4921 gccagatttc cagcacgcgc ttccacgagc agtcatcgac gatccgcatg ccggggaagg
     4981 tgacgacggc gtggactgtg gccgccagcc tctgccccgt cagcccgtac tggctatgca
     5041 gtcgttatcc tggctcgtcg tggctccgtt gaagcagccc gcttcggcgg cgagtttgtt
     5101 ccaaacgcga agtccttcaa gctgcatgcc gatagtatag cggcttcagc gtctcgcata
     5161 gaggccatac ttggtcacga tgacgtcttg aagcggcggt tccgctgctc gaagtgccgg
     5221 caggtcggga aggttcttta cgttcttcag ttgatcgtcc agagagtcgc ccttgggcag
     5281 gtcgagccaa tcacgaatga acgcgtattt cctcttcgcc atctcgaagt cggtcacgta
     5341 cgtggtagca accttcgagt atcttgccgc tcccgcgatg aagtcggcga tgtagatgtc
     5401 gggattcaaa tgcagcgtgt tgcctttcca ttcgaagcca ccggagaggc cccaatcgta
     5461 ggcaatcacg tgctgagtgc ttgagaagcc ctgatgagcc atcacattcc gttgctcgtg
     5521 aagttcagcg acgatcgcag agtacttgcg atcaggcgca gttccgtcga cgaactgctc
     5581 gaggtagaac tccgtagcgt caatgccctt caggccgaaa accttctcac agaccatctg
     5641 catcgccgcg tgagtgccca ggagcgtaag cgtgagcagg tgtttcttcg cggcatcttc
     5701 gacttcgatc agaagcgcat tccgaaccat catgaagatg gtcaacgcga actcgcgatc
     5761 agcacccttt acgaaccgac gaaggcgatc ctcgaaactt tccatcacac cctcctcccc
     5821 atcgagtttc ggaacgagac ctcgcgtcgg cgcaaaccaa tcgacggata tgaaaatgcc
     5881 tgcgaaggtt gagcgctaga aagctgtcgc gaaccagtca acggatgctc ttggctgcgc
     5941 gatagacgac tttccgctcg agaatgattc tttcagtgac ttcgatttca tcgcagttgt
     6001 gtgcaatctg tggatgctgc ggcatctcga agtctgggtg aggattggct tctctagcag
     6061 gtgggatagt ccgagtggga ctcggaacac aatgcaccat ctcggggttc tgcacggcgt
     6121 cttcatgaga tgaatgttgc ttgacataga tcagccaccc acagctcaac gcgataacca
     6181 tgatggcagg actctgaacg acgaacgaac ctatggctgc aacgtccaag cggtccacga
     6241 taatcttctt gagatattcc aggtgatgcg cagtacccgt ggcctgccag aacacaccgg
     6301 ctccgagtga caaccaaccc gcccacgttt ttttcaagat agtcggccgc gcatccatag
     6361 tcccaacccc caccctcatc tactcacgac caatcggcga gctgtctccg gggatcgcgg
     6421 ccaacgatgc gcaatcggcg ccgcggcact aaatcgagga cgttgtcgag tcggatcgcc
     6481 gccgcctgct tcgagaggcc gaaggtttgc gcgatttctg tcgggctgag gtaaccgatc
     6541 tcatcatgga gctgcagcac agcttggagc ggcgcgatga atgcagcggc gaatgcatcg
     6601 ccctgccact ccgtatcgcg gaagatcgcg tgctccgaac gccgattgag cgacggcttg
     6661 aacggatgaa gggcccgtgc gatgagttct ttcgtgtgga gaacgacatg ccc
//
LOCUS       DATLVW010000082         9698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_336053, whole genome shotgun sequence.
ACCESSION   DATLVW010000082 DATLVW010000000
VERSION     DATLVW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336053"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VNN25_02715"
     CDS             complement(<1..279)
                     /locus_tag="VNN25_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXH90467.1"
                     /translation="MIGLEAEVTAPDVASFADDAPADSPNRPSIRTSDPINRRILEVS
                     EDRIRGFVSDPFSTIAELSGVPLPVVTQRIRAMLESGTIRRVRQTLLAT"
     gene            complement(276..1460)
                     /locus_tag="VNN25_02720"
     CDS             complement(276..1460)
                     /locus_tag="VNN25_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH90468.1"
                     /translation="MISVTKLLGGSSYFGDRLRYHDQSAHQTTGARPGSGPVVVWNAT
                     KTCNLECVHCYADAETVKFANELTTAEAKAMIEDLAALNVPALLISGGEPLVRPDIFE
                     LAAYAMSLGVRVTFSTNGTLITKEKAQKIADIGVTYVGISVDGDEERHDRMRCREGAY
                     RDALRGIRNCRDAGIRVGVRFTVTQDNVDEVDSVFRLVENEGIGRLCIYHLVYSGRGS
                     YLEGIDLEAEQKRVLMNKLLEQVERWTAEGREIEVMTVDNHADAPFIYYWLLDRDPVR
                     ADEALSLMRNNGGNRSGIAIAAIDSFGIVHPDQFTSTHAVGSIRERPFSQIWNDSRIQ
                     LLSNLRDRKPLLQGRCATCQWLPCCNGNFRARAEAATGDYWQSDPGCYLLDSEVISGA
                     PE"
     gene            1744..1941
                     /locus_tag="VNN25_02725"
     CDS             1744..1941
                     /locus_tag="VNN25_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXH90469.1"
                     /translation="MFTRKTSFVSLTWVFAVGAVAGAAAALLYAPMTGRKMQKKVADV
                     TDKVIDKVDDIKSAVLKVANG"
     gene            2148..2405
                     /locus_tag="VNN25_02730"
     CDS             2148..2405
                     /locus_tag="VNN25_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HXH90470.1"
                     /translation="MNNLLILAVGFGAAFGTTIAWLPQVGRTWSTRSARDFSWGYLFL
                     FMMGVFLWTMYGVLRHDIVVIVANGVTLLLVMSVGLVKARE"
     gene            2493..3542
                     /locus_tag="VNN25_02735"
     CDS             2493..3542
                     /locus_tag="VNN25_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HXH90471.1"
                     /translation="MTQRDFAFIQTAPGRVFIGWGPFEQLPFRRPGRPAFFIADFFLD
                     DPHPWRHPATWEETTFEDLALRLEDRDAPRVTWEPPPLDDFRALFESARAAMARGDFQ
                     KIVPVIFESGRFAERGDYWHWFFTRLGELPPGLRAYGYSYGTHGVAGATPELLFEGFA
                     RGYRTMALAGTRPVAQAAELLNDPKELREHRFVVDDIVRRLSPFGNIEVGPLGTLRMP
                     KIAHLITPIFFSESGGSERMSFAEMIRRLHPTAALGVSPRNEAGERWLREADHGVKRR
                     TFGAPFGLEREDHRAVALVAIRNLQWDGERVRVGSGAGLIAESRVERELEELREKREQ
                     VKALFGVTAHVEAFA"
     gene            3539..5275
                     /locus_tag="VNN25_02740"
     CDS             3539..5275
                     /locus_tag="VNN25_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXH90472.1"
                     /translation="MTNIAEARRVIEEAVARGATDFCVCAGSRNAPLLAVLGVSDLRV
                     FSFVDERSAAFFALGRAKITGRPTVVITTSGTAVAELLPAAIEAHYSAIPLIFITADR
                     PARYRGTGAPQAIEQEEIFGVYARGSVQHINIEFDEPLIDDAVSRWNAAVPAAGQAAS
                     SPPQRSPSLSRSLRGGEDAAEPAGGDAGVPSAFAEHPLVIVGGLHERDRDRVRDFAIA
                     LNAPVYAEPLSGLREDPALAHLLITAGERMIGRGDFDGVIRIGNVPTARFWRDLDESR
                     RDLSLISFSALPFTGCSRGVVHPIEALPVDVTPRERDEQFFKADHEQAAAIERILDAE
                     PQSELALLRELSRTIPENSRVYLGNSLPIREWDLAATRDARGFAIEANRGANGIDGQL
                     STFFGQCDPSRMNVCVIGDLTAIYDSNAPWIVPQLDRAIDFQIVIINNGGGRIFSRVA
                     SLRKLDESIRERIIENSHDLRFEHWAAMWGMEYSVITNAGDLARSRRLVVSTALDARS
                     ARDAGFLPSGHAMAQLAQLPNRAPFSPTVFVGEKVPKADEGVFLSGKRVIEIRPDPES
                     TQRAWAKFDALW"
     gene            5272..5880
                     /locus_tag="VNN25_02745"
     CDS             5272..5880
                     /locus_tag="VNN25_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH90473.1"
                     /translation="MITALHGFLGAPSDWNFLRDAGFVIDTPPLDAIPPRGDTLLGYS
                     LGGRLALQAILAGARYERAIFISTGLGIEDEAARAARRASDEAWAHRFESDDWDAVIN
                     AWNAQPVLAGPALPRTRDDYDPRALREWSSAALPPVASRLHELTIPTLWIAGARDTKY
                     VAEAHRAASLASNARVAIIENAGHRVPWERPEALITLLRNPF"
     gene            complement(5964..6938)
                     /locus_tag="VNN25_02750"
     CDS             complement(5964..6938)
                     /locus_tag="VNN25_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HXH90474.1"
                     /translation="MPSPIRDFIQHHYRHFNAASLRAAADDYIKHIDGGNKMLLTMAG
                     AMSTAELGISLAEMIRQGKIHAICCTGANLEEDIFNLVAHDHYERVPGYRYLSANDEK
                     ALLDRHMNRVTDTCIPEGEAMRRIESAVLRHWMAKDKEGRRLFPHEFMYEILRDGSIK
                     EFYQIDPKNSWMIAAAEKNLPMFVPGWEDSTLGNMYTGHCITGDVGNVHTVRTGIEYM
                     IELADYYTKTTKDSSIGFFQIGGGIAGDFPICVVPMLHQDLQRTSVPLWGYFCQISDS
                     TTSFGSYSGAVPNEKITWGKLGVDTPSHVIESDASIVAPLMFGYVLGW"
     gene            7167..>9698
                     /locus_tag="VNN25_02755"
     CDS             7167..>9698
                     /locus_tag="VNN25_02755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXH90475.1"
                     /translation="MKQTITLLLLVCATAFADDANLTRSIETDVRTLAAPNMEGRGLG
                     TKGIGLAASYIEGRLHALGLQPAFGKSYRQAFPVKIGVSLGTGNKLEGVDPSAWLPLG
                     FSSAGAFSGPLVFVGYGIDATPIGYNDFDGVDLKGKVALMLRYEPQEKDDNSKFDGRK
                     PSRWSAMRYKVLKARERGAVAVVFVTGPIQDEGKDKIPRLMNDGPESPAGIPVIQVQT
                     SVAQNWLKDSGIDLAQFQKDVDADLKPRSKVIGKIISGVADVKPEFVDGENLAGVLPG
                     RGALANEIVVIGAHYDHLGYGGQGSMKPNVHAIHPGADDNASGDAAVLAIAERLRNQL
                     DDVKNRRTIAVALFSGEEVGLAGSSWFVGHPPSALSSVVAMINLDMVGMMRDNRLIVF
                     GTDSAPQWKEVVDHATEFAKISVTSSGDGYGPSDQTSFYAKQIPVLHFFTGAHDRYHT
                     PEDVAESINYAGMEHVVDFATSVMMHLAAGRATPHYVRAASAPAMEGDSRGYGAYLGT
                     VPDYSAMDATSGGVLLADVRPGSPADKAGIRGKDKIVSIGGTRIENLYDMSYALQDHK
                     PGDTVDIIVLRDGVRTTLRATLTTRGVTPATPPAAAPAKPLVIGAGKPYDKTFDGEKH
                     LKDIRQLTFGGENAEAYFSPDGTKIIYQATVPGAGCDQEYTMDLVTGETKLVSSGKGR
                     TTCGYYRYPQGDRIVYATTEGGDAACPPRPDMSKGYVWPVYKSFDVVEANVDGSNAKK
                     ITTAEGYDAEMTWCHRGGKMIFTSMRDGDLELYEMDEASGKVKRLTNAPGYDGGAFYN
                     ADCTEIVWRANRPVGPALVEDRALLAQGLVKPLHMELFLMNADGTN"
BASE COUNT         1725 a         3091 c         3092 g         1790 t
ORIGIN      
        1 cgtcgccagc agtgtctgcc gcacgcggcg gatcgtgccc gattcgagca tcgcccggat
       61 tcgttgcgtg acgacaggaa gcggcacgcc tgacagctcg gcgatcgtgc tgaacggatc
      121 gctcacgaat ccgcgaatac gatcctccga cacttccaga atgcggcgat tgatcggatc
      181 actggtgcgg atcgacggac ggttaggcga gtcggccggt gcgtcgtcag cgaacgatgc
      241 aacgtccggt gcggtcacct cagcttcgag cccgatcatt ccggcgctcc tgagatgact
      301 tctgaatcga gcaggtaaca ccccggatcg gactgccagt aatcaccggt agccgcctcg
      361 gcgcgcgcac ggaagttgcc gttacagcat ggcagccact ggcaggtcgc gcagcgtcct
      421 tgcagcagag gcttgcgatc gcgcaaattg ctcaggagct ggatccgtga gtcgttccag
      481 atttgcgaga acggccgctc gcgaatcgat ccgaccgcat gggtcgaggt gaactgatct
      541 ggatggacga taccgaagct gtcgatggcg gcgatggcga tgccggagcg gttgccgccg
      601 ttgttgcgca tcagcgagag cgcttcgtcc gcgcgcacgg gatcgcggtc gagcagccag
      661 taatagatga agggggcgtc ggcatggttg tcgacggtca tgacttcgat ctcgcgtccc
      721 tcggcggtcc agcgttcgac ctgttcgagg agcttgttca tcagcacgcg cttctgctcg
      781 gcctcgaggt cgatgccttc gagatacgag ccgcggcctg aatagacgag gtggtaaatg
      841 caaagccggc cgatgccttc gttctccacg agacggaaga cgctatcgac ctcgtcgacg
      901 ttgtcctgcg tcaccgtgaa gcggacgccg acgcggatgc cggcgtcgcg gcagttgcga
      961 atgccgcgca gggcgtcgcg gtacgcgcct tcgcggcagc gcatgcggtc gtggcgctct
     1021 tcgtcgccat cgacgctgat gccgacgtag gtcacgccga tgtcggcgat cttctgcgcc
     1081 ttttctttcg tgatcaaggt gccgttcgtc gagaaggtca cgcgaacgcc gagggacatg
     1141 gcgtaggcgg cgagctcgaa gatgtcgggg cggacgagcg gttcgccgcc ggagatcaga
     1201 agggccggca cattgagcgc cgccagatct tcgatcatcg ccttcgcctc ggccgtcgtc
     1261 agctcgttcg cgaacttgac ggtctccgcg tccgcgtagc agtgaacgca ttcgaggttg
     1321 cacgttttcg tggcgttcca caccacgacc ggtccgctgc cgggccgcgc gccggtggtc
     1381 tggtgcgctg attggtcgtg atagcgaagc ctgtcaccaa agtaagagga gccaccgagt
     1441 agtttcgtca cgctgatcat ttcgtctccg tttgtcgaaa gaaatcatcg gcccggcgtg
     1501 aacaggagaa cgtgacggca tctacgccgc cctgtgacgc gcgacacatt gcgcgggagt
     1561 ggggtggtgt cgggggtcgg gggtcgtaga ttggctcccg actccggtcc cgagccgctt
     1621 ttgctccgac ccccgacccc cgacgcccgc caacactacg aatcccgcgc accagagtgg
     1681 gtgacacggg tcatcgccgc cgcgccgcgc gaaacctatt gtgtggaacg aaaggagcga
     1741 ccaatgttca cacgcaaaac gtcatttgta agtctcacct gggtcttcgc agtcggagcc
     1801 gtcgccggtg ccgcagcggc actgctttat gcaccgatga ccggccggaa gatgcagaag
     1861 aaggtcgccg acgtcaccga caaggtgatc gacaaagtcg acgacatcaa gagcgcggtc
     1921 ctgaaggttg cgaacgggtg accgcgggag tcgtagtcgt gtcgggggtc gggggtcgta
     1981 tcgtcgggtg tcgtagggtt gtcgggggtc gggagtcgta gagtttctac tttccggctc
     2041 cctcgtttgg ctccgactcc cgacccccga ctccccaccc ccgcatcacg cagggatgtg
     2101 acgcccatca tgctgccagc ggcccgcctg cgtcagcgtg gcgctgtatg aacaacctac
     2161 tgattctcgc cgtcggcttt ggagcagcat tcggaacgac gatcgcctgg ctcccgcagg
     2221 tcggacgcac atggagcacg cgcagcgccc gcgatttctc gtggggctac ctcttcctct
     2281 tcatgatggg cgtcttcctc tggacgatgt acggcgtgct ccgccacgac atcgtcgtca
     2341 tcgtcgccaa cggcgtgacg ctgctactgg tgatgtcagt gggcttggtg aaagcgcggg
     2401 agtgagtttt gatccgcaga tgacgcagaa ggagcgcaga taatccttcg cgtcttcgcg
     2461 tcttcgcgtg agcacactac aattcgctcc gcatgacgca gcgcgacttc gccttcatcc
     2521 agaccgcgcc gggacgcgtc ttcatcggat ggggaccgtt cgaacagttg ccgttccggc
     2581 ggcccggccg gcccgcgttc ttcatcgccg acttctttct cgacgatccg catccatggc
     2641 ggcatccggc cacgtgggag gagacgacgt tcgaagatct tgcgttgcgc ctcgaggatc
     2701 gtgatgcgcc gcgcgtgacg tgggaacccc caccgctcga tgacttccgg gcgctcttcg
     2761 aatccgcgcg cgcagccatg gcccgcggcg atttccagaa gatcgttccc gtcatcttcg
     2821 agtcaggccg cttcgccgag cgcggcgact actggcactg gttctttacc cgtctcggcg
     2881 agttgccacc aggcctgcgc gcgtacggct actcgtacgg cacgcacggc gtggccggcg
     2941 cgacaccgga gttgctcttc gagggattcg cgcgcggcta tcggacgatg gcattggccg
     3001 gcacgcgtcc ggttgcgcag gccgcggagc tcctcaacga tccgaaagaa ctgcgcgagc
     3061 atcgcttcgt cgtcgacgac atcgtccgcc gcctttctcc gttcggaaac atcgaagtcg
     3121 gtccgctcgg cacgctgcgc atgccgaaga tcgcgcatct catcacgccg attttcttca
     3181 gcgagagcgg cggctcggag cggatgtcct tcgccgagat gatccgccgc ctccacccca
     3241 ccgccgccct cggcgtttcg ccgcgcaacg aagcggggga gcgatggctg cgcgaggccg
     3301 accacggcgt gaagcgcaga acgttcggcg cgccgttcgg tctggagcgc gaagatcacc
     3361 gcgctgtggc gctcgtggcc atccgcaatc tgcagtggga cggcgagcgt gtgcgcgtcg
     3421 gatcgggcgc aggcctcatc gccgagagcc gcgtcgagcg cgagctggaa gagctgcgcg
     3481 aaaagcgcga gcaggtgaaa gcgctcttcg gcgtcaccgc gcacgtcgaa gcattcgcat
     3541 gaccaacatc gccgaagcac ggcgcgtgat cgaagaggcg gtggctcgcg gcgcgaccga
     3601 tttctgcgtt tgcgcgggct cgagaaatgc gccgctgctg gcagtgctcg gggtaagcga
     3661 tctgcgagtc ttctccttcg tcgatgagcg ctccgctgct ttcttcgcct taggccgcgc
     3721 taaaatcacc ggtcgcccca ccgtcgtcat caccacctcg ggtaccgcag tcgccgagct
     3781 cctccccgcc gccatcgaag cgcactattc cgccatcccg ctgatcttca tcaccgccga
     3841 ccgtcccgct cgataccgcg gcacgggcgc cccacaagca atcgagcagg aagagatttt
     3901 cggcgtctac gcccgcggct cggtgcaaca catcaatatc gagttcgacg agccgctgat
     3961 tgatgacgcg gtgagtcgat ggaacgctgc cgtcccggcg gctggccagg cggcgtcctc
     4021 gccgcctcaa cggagtccgt cactttcccg gtcactccgc ggcggcgagg acgccgccga
     4081 gccagccggc ggggacgccg gcgttccgtc cgcttttgcg gagcacccgc tcgtgattgt
     4141 cggtggtctg cacgaacgcg accgcgatcg ggttcgtgac tttgcgatcg cgctcaatgc
     4201 gccggtctat gcggagccgc tctccggcct gcgcgaagat ccggcgctcg cacatctttt
     4261 gattaccgcg ggcgagcgaa tgatcggacg cggcgatttc gatggcgtta tccgcatcgg
     4321 caatgtcccc accgcgcgct tctggcgcga tctcgacgaa tcacgccgcg atctttcgct
     4381 catcagtttc agcgcgttgc cgttcactgg atgcagccga ggagtggtcc atccgatcga
     4441 agccttgccg gtcgatgtga ctccacgcga acgcgatgaa caattcttta aagccgacca
     4501 cgagcaagcc gctgcgatcg aacgaattct cgacgccgag ccgcaatcgg agctggcgct
     4561 tcttcgcgag ctctcacgca ccatccccga aaactcccgc gtctacctcg gcaactccct
     4621 ccccatccgc gaatgggatc tcgccgccac gcgcgacgcc cgcggcttcg ctatcgaagc
     4681 caaccgcggc gccaacggca tcgacggcca gctctccaca ttcttcggcc agtgcgatcc
     4741 gtcgcgcatg aacgtctgcg tcatcggcga cctcaccgcc atctacgaca gcaacgcccc
     4801 ctggatcgtc ccacaactcg atcgagcgat cgacttccaa atagtcatca tcaacaacgg
     4861 cggcggccgc atcttcagcc gcgtggcctc gctgcgcaag ctcgatgaat cgatacgcga
     4921 gcgcatcatc gaaaacagcc acgacctccg cttcgaacat tgggccgcga tgtgggggat
     4981 ggagtactcc gtcatcacga acgcgggcga tctcgcgagg tcgcgcaggc tcgtggtctc
     5041 gacggcgctc gatgcgaggt ccgcgcgcga cgcgggattt ctgcccagtg gacatgcgat
     5101 ggcacagctg gctcagctcc ccaatcgagc gcccttctcc cccacggttt ttgtggggga
     5161 gaaggtgccg aaggcggatg agggggtatt tctaagcgga aaacgtgtga tcgagatccg
     5221 ccccgatcct gaatccaccc aacgagcctg ggcaaaattc gacgcgctat ggtgatcacc
     5281 gctctccacg gcttcctcgg cgcgccgagc gactggaact tcctccgcga cgccggcttc
     5341 gtcatcgaca cgccgccact cgatgccatc cccccacgcg gcgacaccct tctcggctac
     5401 tccctgggcg gccgtctcgc tctgcaagcg atcctcgccg gtgcgcgcta cgaacgcgcc
     5461 atcttcatct ccaccggcct cggcatcgaa gacgaagccg cccgcgccgc ccgccgcgcc
     5521 agcgacgaag cctgggccca tcgtttcgaa tccgacgatt gggacgcggt gatcaacgcg
     5581 tggaacgccc agccagtcct cgccggcccc gcgcttccgc gcacgcgcga cgactacgac
     5641 ccccgcgccc tccgcgaatg gtcatccgcc gccctcccgc ccgtcgcctc gcgcctccac
     5701 gagctgacca tccccaccct ctggatcgcc ggcgcccgcg acaccaaata cgtcgccgaa
     5761 gcgcaccgcg ccgcctcgct cgcgtccaac gcgcgcgtgg cgatcatcga gaatgccgga
     5821 catcgcgttc cgtgggagcg acccgaggca ttgatcacgc tgctgcgcaa ccccttttaa
     5881 ccacagagac acagaggtta cagagatacc gaatttgaac tctgtgatct ctgtgtctct
     5941 gtggttaaag attctaaaaa actctaccac cccaacacat acccaaacat cagcggcgcg
     6001 acaatcgagg catccgattc gatcacgtgg cttggtgtgt cgacgccgag tttgccccac
     6061 gtaatctttt cgttcggcac tgcgccggag tacgagccga agctggttgt gctgtcactg
     6121 atctggcaga aatagcccca tagcgggacg ctggttcgtt gcaggtcctg gtggagcatg
     6181 gggacgacgc agatggggaa gtcgccggcg atgccgccgc cgatctggaa gaagccgatc
     6241 gaggagtcct tcgtcgtctt tgtgtagtag tcggccagct cgatcatgta ttcgatgcct
     6301 gtgcggacgg tatgcacgtt tccgacgtcg ccggtgatgc agtggccggt gtacatgttg
     6361 ccgagggtgg agtcttccca gccggggacg aacatgggga ggttcttttc ggcggcggcg
     6421 atcatccacg aattcttcgg atcgatctgg tagaactctt tgatcgagcc gtcgcgaagg
     6481 atctcgtaca tgaactcgtg cgggaaaagg cggcggcctt ctttgtcctt ggccatccag
     6541 tgtcgcaaca ccgccgattc gatccggcgc atcgcctcgc cctcggggat gcaggtgtcg
     6601 gtgacgcgat tcatatgccg gtcgagcagc gccttttcat cgttcgccga caggtagcga
     6661 tagcccggca ctcgctcgta gtgatcgtgg gcgacgaggt tgaagatgtc ttcctcgagg
     6721 ttcgcaccgg tgcagcagat cgcgtggatc ttcccctgcc ggatcatctc ggcgagcgaa
     6781 atcccaagct cggctgtcga catcgccccg gccatcgtca ggagcatctt gttgccgccg
     6841 tcgatgtgct tgatgtagtc gtcggcggcg gcgcggagtg aggcggcgtt gaagtggcgg
     6901 tagtggtgct ggatgaagtc gcgaatcgga ctcggcatgc cgcggattct atatgcgctt
     6961 gccacggtag ttgtcctcga gtggttgaaa accgtttcac gcggagacgc ggagacgcgg
     7021 ggtgttttct ctccatctcc gcgtactccg cgcctccgcg tgaccggtct ctccttcgat
     7081 gaacagaata tggacacgtg cgggatatca catcggattg tgaccaaccg tatggccggg
     7141 acactctgga gcgttacgat gtccctatga aacaaaccat cactctgctg ctcctcgtgt
     7201 gcgcgaccgc gtttgcggat gacgccaatc tgaccaggtc gatcgagacc gacgtccgga
     7261 cgctcgctgc cccgaacatg gaaggccgcg ggctcggcac gaagggcatc ggcctcgcgg
     7321 cgagttacat cgaggggcgg ttgcacgcgc tcgggttgca gccggcgttc ggcaagtcgt
     7381 accggcaggc gtttccggtg aagatcgggg tttcgctcgg cacgggcaac aagctcgaag
     7441 gcgttgatcc gtcggcgtgg ctgccgctgg ggttctccag cgccggcgca ttttccggtc
     7501 cgctcgtgtt cgtcggctac ggcatcgacg ccacgccgat cggctacaac gactttgacg
     7561 gcgtcgatct caaaggcaaa gtcgcgctga tgctgcgcta cgagccgcag gagaaggacg
     7621 acaactcgaa gtttgatggc cgcaagccga gccgctggtc ggcgatgcgc tacaaggttt
     7681 tgaaggcgcg cgagcgtggc gcggttgcgg tggtcttcgt caccggcccg attcaggacg
     7741 agggcaaaga caagatccca cgcctcatga acgacggccc ggagagtccg gccggcattc
     7801 cagtcatcca ggtgcaaacc tcggtcgcgc agaactggct caaggattcg ggcatcgacc
     7861 tggcgcagtt tcagaaggat gtcgatgccg atctgaagcc gcgctcgaaa gtgatcggca
     7921 agatcatcag cggtgtcgcc gatgtgaagc cggagttcgt tgacggcgag aatctcgctg
     7981 gcgtgcttcc gggccgcggg gcactcgcaa acgagatcgt cgtcatcggc gcgcactacg
     8041 atcacctcgg ctacggcgga cagggatcga tgaagcctaa cgtccacgcc attcatcccg
     8101 gcgcggatga caacgcgtcc ggcgatgccg cggtgctggc gatcgcagag cgcctgcgca
     8161 atcaactcga cgacgtgaag aaccggcgca cgatcgccgt ggcgctcttc tccggcgaag
     8221 aagtcggtct ggccggctcg tcgtggttcg tcgggcatcc gccgtccgcg ttgtcgagcg
     8281 tggtggccat gatcaatctc gacatggtcg ggatgatgcg cgacaaccgc ctgatcgtgt
     8341 tcggaacgga ctccgcgccg cagtggaaag aggtcgtcga tcacgcgacg gagtttgcga
     8401 agatcagcgt tacatcgagc ggcgacggat acggcccctc ggatcagacg agcttttacg
     8461 cgaaacagat tccagtgctg cacttcttca ccggtgcgca cgatcgctat cacacgcccg
     8521 aggatgtggc ggagagcatc aactacgcgg ggatggagca cgttgtcgat tttgcgacga
     8581 gcgtgatgat gcatctggcg gcgggacgtg cgacgccgca ctacgtgcgc gcggcgtcgg
     8641 ccccggcgat ggaaggcgat tcgcgcggct acggtgccta cctcggcacg gttcccgact
     8701 actcggcgat ggacgccacg agcggcggcg tgctcctcgc ggatgtccgc ccgggctctc
     8761 cggccgacaa ggccggaatc cgcggcaagg acaagatcgt ctcgatcggc ggcacacgca
     8821 tcgagaatct gtacgacatg agctacgcgc tgcaggatca caagccgggc gatacggtcg
     8881 acatcatcgt gctgcgcgat ggcgtgcgca cgacattgcg cgcgacattg acgacccgag
     8941 gcgtgacgcc ggcaactccc cccgctgcgg cgcccgcgaa accattggtc atcggggcgg
     9001 gcaagccgta cgacaagacg ttcgatggcg aaaagcatct gaaagacatt cgccagctca
     9061 cgttcggcgg cgagaacgcc gaggcgtatt tcagcccgga cgggacgaag atcatctacc
     9121 aggccacggt tccgggcgcg ggctgcgatc aggagtacac gatggatctc gtgaccggcg
     9181 agacaaagct ggtctcgagc ggcaaaggcc ggacgacgtg cggctactac cgttatccgc
     9241 aaggcgatcg catcgtctac gcgactacgg aagggggcga cgctgcctgt ccgccgcggc
     9301 cggatatgtc gaagggttac gtctggccgg tgtacaagtc gttcgatgtc gtcgaagcga
     9361 atgtcgatgg atcgaacgcg aagaagatca cgaccgccga gggctacgac gcggagatga
     9421 cgtggtgcca tcgcggcggg aagatgatct tcacgtcgat gcgcgacggc gatctggagt
     9481 tgtatgagat ggacgaggcg agcgggaagg tcaagcggct gacgaacgcg cccggctacg
     9541 acggcggcgc gttttacaat gccgactgca cggagatcgt ctggcgcgcc aatcgtccgg
     9601 tcggtccggc gctcgtcgaa gaccgcgcgc ttctggcgca ggggctggtc aagccgctgc
     9661 acatggagct cttcctgatg aacgccgacg gcacgaac
//
LOCUS       DATLVW010000083         5067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_250900, whole genome shotgun sequence.
ACCESSION   DATLVW010000083 DATLVW010000000
VERSION     DATLVW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250900"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNN25_02760"
     CDS             <1..271
                     /locus_tag="VNN25_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HXH90476.1"
                     /translation="KYTKEKEDIKKQAEGFQKEYDALNFHDDQFDMNEACISVAIALF
                     GVTALTKKKWLLGLASVFMFFGVLFGLAGFLGWSIHPDFLARILS"
     gene            complement(335..646)
                     /locus_tag="VNN25_02765"
     CDS             complement(335..646)
                     /locus_tag="VNN25_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH90477.1"
                     /translation="MRSRFHKGAKADLAEDIEYYDLATSGLGDRFLSEVQAAVAFLEN
                     FPEGARTISGDVRGKGLTRFPHTLLYVIEKNEVVILSVAHQRQDLLEWLKIVRVRRTR
                     G"
     gene            complement(643..879)
                     /locus_tag="VNN25_02770"
     CDS             complement(643..879)
                     /locus_tag="VNN25_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH90478.1"
                     /translation="MGHRKLDDLAMEALDLGRESRAALAKRLLDSLDEPSADEVGPGW
                     LAEAERRYQEIKDGTTRTIPSEEVFAELAARHQK"
     gene            complement(939..1133)
                     /locus_tag="VNN25_02775"
     CDS             complement(939..1133)
                     /locus_tag="VNN25_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH90479.1"
                     /translation="MSEFMVDVEREDDGRWIGEVTDLPGVLCYGATRDEAVAKAKALA
                     FRVLADRLDHGEDIPEPHDV"
     gene            complement(1153..4245)
                     /locus_tag="VNN25_02780"
     CDS             complement(1153..4245)
                     /locus_tag="VNN25_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXH90480.1"
                     /translation="MSFSEPFIRRPVGTALLSVAILMAGALGYRNLPVAPLPQVEFPT
                     ISVSAGLPGADPETMASSVATPLERQFGRIAGVTQMTSNSSSGSTNVTLQFELDRDID
                     AAARDVQAAINAARGQLPANLPSNPNWRKVNPADAPIMILGLTSDVKTQPQMYDVADS
                     VLAQKIAQIDGIGQVFVGGSSRPAVRVEVNPLQLSKLNVGIDQVRAAIGTANANRPKG
                     SISGPGNVWMLQTNDQLFKANEYRSLIVASRDGKPVILSEVARVEDSVENINSAGSVN
                     GKPAISLVLFRQPGANIIGTVDRVRAMIPQLQATIPPSIHLDVVLDRTGTIRASVRDV
                     EVTLSISILLVILVVFVFLRSGWATVIPSVAVPLSLIGTFGVMYLLGYSLDNLSLMAL
                     TISTGFVVDDAIVVIENISRHLEDGMKPLDAALRGAREIGFTVFSMSTSLIAVFIPIL
                     LMGGIVGRLFREFAVTLSVAIVFSLIISLTTTPMMCAKFLRSEKERKHGRLYRASERA
                     FDHLRSGYEHTLDFVLDHRLPVLLVTLATIGFTVYLYVVIPKGFFPSQDTGRLNGNVQ
                     GAQDTSFDALITKLNQFMRIVEKDPAVESVVSFTGGGRGGGNSASMFVTLKPLEVRKA
                     SAQEIIARLRPKTSHIPGAQLFLTAVQDVRVGGRQSNAEYQYTLQGDNVADVMTWAPK
                     LSKAMQKMKELKDVSSDQQNQGLQASVVIDRQTAARLGISLASIDSTLYDAFGQRQVS
                     VMYSGMNQYHVVMEVDPKFSGSPAALSNVYVHSSTGREVPLSTIAHFGLDQTPLSVPH
                     QGQFPAVTISFNLSPGVSLGVATAAIEDEERKMGMPSSIHPGFQGTAQAFQQSLSTEP
                     MLILAALLAVYIVLGILYESLVHPVTILSTIPSAGVGALIALLLTNTEFTIIALIGII
                     LLIGIVKKNAIMMIDFALEAERKHGKSARDAIREACLLRFRPIMMTTMAALFGGLPLA
                     LGGGTGSELRRPLGIAIVGGLIMSQMLTLFTTPVTYMYLDKLRLRGREKQPAADPVRA
                     "
     gene            complement(4454..>5067)
                     /locus_tag="VNN25_02785"
     CDS             complement(4454..>5067)
                     /locus_tag="VNN25_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH90481.1"
                     /translation="PASIHAEFQGTAAVFRSSLANEGYLILAALITVYIVLGVLYESY
                     IHPLTILSTLPSAGVGAILALMLFHMELSVVALIGIILLIGIVKKNAIMMIDFALEAE
                     REQNKPAQEAIYDACLLRFRPIMMTTFAALFGGLPLALGTGTGSELRHPLGVTIVGGL
                     LVSQLLTLYTTPVVYIWFDNLAKRMKRHVRVGNPVHGVQPAEG"
BASE COUNT         1001 a         1431 c         1659 g          976 t
ORIGIN      
        1 gaagtacacg aaggagaaag aggacatcaa gaaacaagcc gagggttttc agaaagagta
       61 cgacgcactc aattttcacg acgatcagtt cgacatgaat gaagcctgca tctccgtcgc
      121 catcgccctc ttcggcgtca cggccctgac gaagaagaaa tggctcctcg gcctggcctc
      181 ggtcttcatg ttcttcggcg tgttgtttgg cctggcgggg ttccttggct ggagcattca
      241 ccccgatttt ctggcgagga tcttgtcgta ggttgacgtc gggctcgatc actgtcgaaa
      301 cccgaataga ggttggcgtc cttgctgtaa ttgtttagcc gcgcgtgcgc cgtacgcgaa
      361 cgatcttcag ccactccagc aaatcctggc gttgatgagc gacagaaagg atgaccacct
      421 cgttcttctc gatcacgtac agcaatgtgt gtggaaaacg ggtgagccct ttgcctcgga
      481 catctccaga aatggttcga gcgccttccg gaaagttctc aagaaaagcg actgcggctt
      541 gaacttccga caggaatcga tcgccgagtc cggaagtagc gaggtcgtaa tactcgatat
      601 cctcggcgag atccgcttta gcacccttgt ggaaccggga tctcactttt ggtgacgggc
      661 cgccagctcg gcgaacactt cttcggacgg aatggtgcgc gtcgtaccgt ccttgatctc
      721 ctggtatcga cgctccgctt cagctaacca gcccgggcca acttcatccg cagaaggttc
      781 atccaaactg tcgagcagcc gtttagcaag cgctgcccgt gactcacgac cgagatcgag
      841 cgcttccatg gcgaggtcgt ccagtttccg atggcccata gaggtgattc tactgcgcaa
      901 gccggtcgtt ttcgacctgc cccctccttt ttgcgcgatt aaacgtcgtg aggctccggg
      961 atgtcttcgc cgtgatcgag gcggtcagcg agaacgcgga acgcaagagc tttggctttg
     1021 gcgacggctt cgtcgcgcgt ggcgccatag caaaggactc cgggcagatc ggtcacttct
     1081 ccgatccagc gaccatcgtc ttctcgctcg acgtcgacca tgaattcgct catctaagtg
     1141 attctatgac gcctacgccc tcaccggatc cgccgccggt tgcttctcgc gtccccgtaa
     1201 ccgcagcttg tcgaggtaca tatacgtcac gggcgtggtg aagagggtga gcatctggct
     1261 catgatcagg ccgccgacga tggcgatgcc gagggggcgg cgaagctcgc tgccggtgcc
     1321 gccgccgaga gctaggggaa ggccgccgaa gagcgcggcc atcgtcgtca tcatgatcgg
     1381 acggaagcgg aggaggcagg cttcgcggat ggcatcacgg gcgctcttgc cgtgcttgcg
     1441 ctcggcttcg agggcgaagt cgatcatcat gatggcgttc ttcttgacga tgccgatcag
     1501 cagaatgatt ccgatcagcg cgatgatcgt gaactccgtg ttggtcaaca gcaacgcgat
     1561 cagcgcgccc acgccggccg acgggatcgt ggagaggatc gtgaccggat gcacgagact
     1621 ctcgtagagg atgccgagga cgatgtagac agcgagaagc gcggcgagga tcaacatcgg
     1681 ttccgtcgac agcgattgct gaaacgcctg tgccgtcccc tggaagccgg gatgaatcga
     1741 tgacggcatc cccatcttcc tttcctcatc ttcgatggcg gcggtggcga cgccgagcga
     1801 gacgccgggc gacaggttga aggagatggt tacggcgggg aactggccct ggtgcggaac
     1861 ggagagcggc gtctgatcga gaccgaagtg agcgatggtg ctgagcggca cttcgcgtcc
     1921 ggtcgacgag tgaacgtaga cgttgctcaa tgccgcaggt gagccgctga acttcggatc
     1981 gacttccatc accacgtgat actggttcat tccgctgtac atgaccgaga cctgccgctg
     2041 accgaaggcg tcatagagcg tgctgtcgat cgaggcgagc gagatgccga ggcgtgcggc
     2101 agtctggcgg tcgatgacca ccgaggcctg caacccctga ttctgctgat cgctggaaac
     2161 gtctttcagc tccttcattt tctgcatggc tttcgagagt ttcggcgccc atgtcatgac
     2221 gtcggccacg ttgtcgccct ggagcgtgta ctgatactcg gcattgctct gacgaccgcc
     2281 gacgcgcacg tcctgcacgg cggtgaggaa gagttgggcg ccgggaatgt gtgacgtctt
     2341 cgggcggagc cgggcgatga tctcctgtgc cgaggccttg cggacctcca gcggcttgag
     2401 cgtgacgaac atgctggccg agttgccgcc tccgcggccg ccgccggtga aggagacgac
     2461 gctctcgacc gccggatcct tctcaacgat gcgcatgaac tgattgagct tcgtgatcag
     2521 cgcgtcgaac gatgtgtcct gagcgccctg cacgttgcca ttcagtcgtc cggtgtcctg
     2581 cgaaggaaag aagcccttcg gaatgacgac gtagaggtag acggtgaaac cgatcgtggc
     2641 cagcgtgacg aggaggacgg gcaggcggtg gtcgagcaca aagtcgagcg tgtgctcgta
     2701 gccgcttcgg aggtgatcga aagcgcgctc gctggcgcga tagaggcggc cgtgcttccg
     2761 ctccttttcg gagcgaagga acttggcgca catcatcggt gtcgtcgtca gcgagatgat
     2821 cagagaaaag acgatggcca cgctcagcgt gacggcgaac tcgcggaaga ggcggccgac
     2881 gatgccgccc atgagcagga tggggatgaa gacggcgatg agcgacgtgc tcatggagaa
     2941 gacggtgaag ccgatctcgc gtgcgccgcg aagtgcggcg tcgagcggct tcatgccgtc
     3001 ctcgagatga cggctgatgt tctcgatcac gacgatggca tcgtcgacga cgaatcccgt
     3061 cgaaatggtc agggccatga gcgagaggtt gtcgagcgag tagccgagga gatacatcac
     3121 gccgaaggtg ccgatgagcg agagcggcac ggccacactc ggaatgactg tggcccagcc
     3181 gctgcgaagg aagacgaaaa cgacgaggat gacgagaaga atcgagatgc tcagtgtcac
     3241 ctcgacgtcg cgcaccgagg cgcggatcgt gccggtgcgg tcgaggacga catcgagatg
     3301 gatcgacggc gggatcgtgg cctgcagttg cggaatcatg gcccggacgc ggtcgacggt
     3361 gccgatgatg ttcgctccag gctgacggaa gaggaccagg gaaatggccg gcttgccgtt
     3421 caccgatccc gcactattga tattctcgac cgagtcctcc acccttgcca cctcggagag
     3481 gatcaccggc ttgccgtcgc gcgaggccac gatgagactc cggtattcgt tggccttgaa
     3541 gagctgatcg ttcgtctgca gcatccagac gttgcctggg ccggagatcg atccttttgg
     3601 gcggttggcg ttggcagtgc cgatcgccgc gcgcacctga tcgatgccga cattgagctt
     3661 gctgagctgc agcggattga cttcgacgcg gacggccgga cgcgagctgc cgccgacgaa
     3721 gacctgtccg atcccgtcga tctgggcgat cttctgagcc agcacggaat cggcaacgtc
     3781 gtacatctgc ggctgggttt ttacgtccga ggtgaggccg agaatcatga tcggcgcgtc
     3841 ggcgggattg actttgcgcc agttcggatt cgacggcaga ttcgccggaa gctggccgcg
     3901 cgcggcgttg atggccgcct gcacgtcgcg cgcggcggcg tcgatgtcgc ggtcgagctc
     3961 gaactgcaga gtgacgttcg tcgagcccga ggaactgttc gaggtcattt gcgtcacgcc
     4021 ggcgatgcgt ccgaattgac gttccagcgg cgtggccacg gacgaagcca tcgtctccgg
     4081 atcggcgccc ggcaatcccg cgctcacgga gatcgtcgga aattcgacct gcggcaaggg
     4141 cgcgaccgga aggttcctgt agccgagcgc ccccgccatc aggatcgcaa cgctcaggag
     4201 cgccgtgcct accggtctgc ggatgaatgg ctcggagaag ctcatgcgga aggatgaagg
     4261 cagaaggatg aaggatgaag gttcgaagaa tgaatgaggc gcgcagagag acagagatca
     4321 gagacaagag acgagagatc agagacaaga gacaagagat cagaaggaaa cgttgctctc
     4381 atctctgatc tctcatctct gatctctgtc ctcgtaaccc ttgactcagc actcatcctt
     4441 catccttctc ccctcatcct tcggctggct gtactccgtg caccggattg ccgacgcgca
     4501 cgtggcgttt catccgtttg gcgagattgt cgaaccagat gtagaccacc ggcgttgtgt
     4561 agagcgtgag gagctggctg acgagcaggc cgccgacgat cgtgacgccg aggggatggc
     4621 gcagctctga gccggtgccg gtgccgagcg cgagggggag gccgccgaag agtgcggcga
     4681 acgtcgtcat catgattgga cggaagcgca gcaggcaggc gtcgtagatc gcttcttgcg
     4741 ccggcttgtt ctgttcgcgc tcagcctcga gcgcgaagtc gatcatcatg atcgcgttct
     4801 tcttcacgat gccgatgagc aggatgatgc cgatcagcgc gacgacgctc aactccatgt
     4861 ggaagagcat cagcgccagg atcgcgccga cgccggccga cgggagcgtc gacagaatcg
     4921 tgagtggatg gatgtagctt tcgtacaaca cgccgaggac gatgtagacg gtgattagcg
     4981 cggcgaggat gaggtagccc tcgtttgcca gcgagctgcg gaagaccgcg gcggtgccct
     5041 gaaattcggc gtggatgctg gccggca
//
LOCUS       DATLVW010000084         3319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_210056, whole genome shotgun sequence.
ACCESSION   DATLVW010000084 DATLVW010000000
VERSION     DATLVW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210056"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VNN25_02790"
     CDS             complement(<1..560)
                     /locus_tag="VNN25_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXH90482.1"
                     /translation="MKRVLAASLLFAVSSFAQSTPQQPVTINDQIVVTASSLPESVQA
                     TPAAVTVITRADINARAALDVADVLREVPGLVLARSGSPGKATSLFTRGAASTQTLVL
                     WNGIVLNNPYFSGYDWGRFSTAGVEQIEVVRGPYSALYGSEAMAGVVNVLTAPRSSGV
                     HGDFESGGHGLRNAALSGDYAAESIQA"
     regulatory      complement(592..780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            929..1267
                     /locus_tag="VNN25_02795"
     CDS             929..1267
                     /locus_tag="VNN25_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90483.1"
                     /translation="MNILHAITLAALLHHAPMTAPVCHKGTVSYKFVGTPGATFVYAG
                     DRYSIPANGSIELLTSDTVYLAANGRSLPLDVWPIDAFGTRTVPVQAAPADSSAGQVA
                     PVNPLNTVNN"
     gene            complement(1569..2885)
                     /locus_tag="VNN25_02800"
     CDS             complement(1569..2885)
                     /locus_tag="VNN25_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH90484.1"
                     /translation="MSKRFAFVTSALLALAASTFAQSVIPNNPGKKLTFAIRNATIVP
                     VTSAPIPNGTIVFANGVITAVGTNAAIPAGATVIDGTGLFVYPGLIDSGSHVGLEEIS
                     AVPGTVDTAELGDINPNARAEVAVNPHSNVIPVTRVNGITSVVTEPEGGIISGSSAMI
                     QLAGWTPKEMTLKAPLAMHIHFPRLRTAAFDEVPQDEEAAKEATKNYTKQIDKLRDTL
                     RDAQSYAKAAAARTNHPDVKRFDRDLILEALVPVVEGREPVVMHANLERDIRAAIKFA
                     DEFKLKVILADAEDVAKVIPELKSHNIPVILGPILSLPPREDDPYDLVYGNAKTLFDA
                     GIPFAIQSQDSHNARNLPYQAAACAAFGLPKDAALRAVTIAPAQIFGMADRIGSLETG
                     KLANIIMTDGDPLEIVTHVKRLFIGGEDISLETNQTLLWEKFKARP"
     gene            complement(2878..>3319)
                     /locus_tag="VNN25_02805"
     CDS             complement(2878..>3319)
                     /locus_tag="VNN25_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH90485.1"
                     /translation="VALMDRAGVLTSVNSDSAELARHLNQDAAKAMKYGGLSEEEALK
                     LCTLNGAKQLRLDHRLGSIDTGKDADLVIWNGHPLSAYSRVETTFVDGEIVFDRQRDL
                     AMRQQMATEKKDRLKKEADEEAKNKKSSRPGKTDDEPKPEVTNE"
BASE COUNT          577 a         1002 c         1071 g          669 t
ORIGIN      
        1 gcctggatgg actcggccgc gtagtccccg ctcagggccg cattgcgcag tccgtggccg
       61 ccgctctcga agtcgccgtg aacgccgctg cttcgcggcg ccgtcagtac attgacgacg
      121 cctgccatcg cctcggaacc ataaagcgcg gagtagggcc cgcgaacgac ttcgatctgt
      181 tcgacgccgg cggtcgagaa gcgtccccag tcgtagccgg agaagtaggg attgttcagg
      241 acgatcccgt tccagagcac gagcgtctgc gtcgatgcag cgccgcgcgt gaagagcgag
      301 gtggctttgc cgggcgagcc gctgcgggcg aggacgaggc cggggacttc gcgcagaacg
      361 tctgcgacgt cgagcgcggc acgagcattg atatcggcgc gcgtgatgac ggtgaccgcg
      421 gccggcgtgg cctgcacgga ctcgggcagc gacgacgcag tcacaacgat ctgatcgttg
      481 atagttacgg gttgttgtgg agtggactgg gcgaacgagg aaacagcaaa aagcagagac
      541 gcagcgagaa cacgcttcat gacgactcct tccctccgaa gaaggccgat caaaagtggg
      601 ttccgagcca gccggtctcc tggcttccgg gtcatcctcg tcgcgccgcc ttcccgcatc
      661 gagcgatgca gtggctttcg tggagcgagt tgtcgccggt cacagtggcg ggggccgcgc
      721 cggcatcaca ccggcttccc gttcactgac tcagaggaaa ttgggaaaga actgtggggg
      781 actatacacc gatgggatag ggagacgatg cgtccgatat tcgcacgctc tgtcttgatt
      841 caggaatgcc ttcaatcgca gttttgagct aaatcattga tttgattgaa aagttcgtct
      901 ggtacctgcc ttggagtgtg tccagtgcat gaacatactg cacgcaatca ccctcgccgc
      961 tctcctccat cacgcaccga tgacggctcc ggtttgccac aagggaacgg tgtcgtacaa
     1021 gttcgtcgga acgcccggcg caacgttcgt ttacgcaggc gatcgttact cgatcccggc
     1081 gaacggctcg atcgagctgc tcaccagcga cacggtctat ctagccgcga acggccgctc
     1141 gctgccactg gatgtctggc cgatcgatgc gttcggaact cggacggttc cggtgcaggc
     1201 ggcgcccgcc gattcttcag cgggacaggt tgcgccggtc aatccgctga acaccgtcaa
     1261 caactagcgc gcttgcgagc gggagaacgt cagatgaccc agatcggaca gttcgcgacc
     1321 gatacatcga tggaccttcg cggcggccgc cagtcgtggc gtgcgctcag catgctctcg
     1381 tcggattcgt tcatcatctg gcgactgcgg cgatcatcct tcgctgcgca cttcacgaac
     1441 gcgacgcgag caatgcattg cgcgttgaga gtgcgctgtc attccgattc tcacgcgaag
     1501 gcgcgaagac cgcgaagaag agcatcgcag cctcaacccc ttcgcgtctt cgcgtgagaa
     1561 cgtcttagtc acggccgcgc tttgaacttc tcccacaaca gcgtctggtt cgtctcgagc
     1621 gaaatgtcct ctccgccgat gaacaggcgc ttcacgtgcg tcacgatctc cagcggatcg
     1681 ccgtccgtca tgatgatgtt ggccagcttg cctgtctcga gtgatccgat ccggtcggcc
     1741 attccgaaga tctgcgccgg tgcgattgtc accgcgcgca gggccgcgtc tttcggcaag
     1801 ccgaacgccg cgcacgccgc cgcctgatac ggcagattgc gcgcgttgtg cgaatcctgc
     1861 gactgaatcg cgaacgggat cccggcatcg aacagggtct tggcgttgcc gtagacgaga
     1921 tcgtacggat cgtcctcgcg cggcggaagc gacaggatcg gcccgaggat gactggaatg
     1981 ttgtgcgact tcagttccgg aatcaccttg gctacatcct cggcgtccgc gagaatcacc
     2041 ttcagcttga actcgtccgc gaacttgatg gccgcgcgga tgtcgcgctc caggttcgcg
     2101 tgcatcacca ccggctcgcg cccctccact accggaacga gcgcctcgag gatcagatcg
     2161 cggtcgaagc gtttgacgtc cggatgattc gtgcgcgccg ccgctgcctt cgcgtacgac
     2221 tgcgcgtcgc ggagagtgtc gcgcaacttg tcgatctgct tggtgtagtt cttcgtcgct
     2281 tctttcgccg cttcttcgtc ctgcggaacc tcatcgaacg cggcagttcg caggcggggg
     2341 aagtggatgt gcatcgccag tggagccttg agcgtcatct ccttcggcgt ccatccggcg
     2401 agctggatca tcgcggacga gccggagatg atgccgccct cgggttcggt gacaacgctg
     2461 gtgatgccgt tcacgcgcgt gaccggaata acgttgctat gcggattcac cgcaacctcg
     2521 gcgcgcgcat tcggattgat gtcgccgagc tccgcggtgt cgacggtgcc ggggacagcg
     2581 ctgatctctt ccagcccgac atgcgagccg ctgtcgatca gaccgggata gacgaagagg
     2641 cccgtaccat cgatgaccgt cgcgcccgcg ggaatggctg cgttcgtgcc gaccgcggtg
     2701 atgacgccat tcgcgaagac gatcgtgccg ttggggatgg gtgccgaggt gacggggacg
     2761 atcgtggcgt tgcggatggc gaaggtgagt ttcttgccgg ggttgttcgg gatgacggat
     2821 tgggcgaagg tcgatgccgc gagggcgaga agcgcagatg taacgaacgc gaaccgctta
     2881 ctcattggtc acctccggct ttggctcgtc gtcagtcttg ccggggcgcg aggacttctt
     2941 gttcttcgct tcttcgtcgg cttctttctt caatcgatcc ttcttttccg tcgccatctg
     3001 ctgacgcatg gccaggtcgc gctggcggtc gaagacgatc tcgccgtcga cgaacgttgt
     3061 ttcgacgcgt gagtacgcgg acagcggatg gccgttccag atgacgagat cggcgtcctt
     3121 gccggtgtcg atcgagccga ggcggtgatc gaggcggagt tgtttggcgc cgttcagcgt
     3181 gcagagtttc agcgcctcct cctcggataa cccgccgtac ttcatcgctt tggccgcgtc
     3241 ctgattgaga tggcgcgcca gctcggcgga gtcggagttg accgaggtga gcacgccggc
     3301 gcgatccatg agggcgacg
//
LOCUS       DATLVW010000085        22003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_46406, whole genome shotgun sequence.
ACCESSION   DATLVW010000085 DATLVW010000000
VERSION     DATLVW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22003
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46406"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3699
                     /locus_tag="VNN25_02810"
     CDS             <1..3699
                     /locus_tag="VNN25_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HXH90486.1"
                     /translation="SLVVNPPSPSIGLLKQVGTSNTGPWNSFLAIASGPVFYRFTVEN
                     TGDVPLTLGPTTLTDNNVDVSACNAGFNGLILPVAVAGNDNHIVTCVVGPVASSPGAH
                     TNTASAAATFNGNPVTSPNDSATYATTSLTLVKSATESTFTLPGDTLHYSYLVTNSGF
                     APLQGPVTVADNKTTVTCPPVNTVGDLDNFLDPGESITCTATYIVTPADVSNAQVTNT
                     AQATADSVNSNSSSKMVPLSTSADVSIDKSLTTAAPYLTGQTINYTLTVANAGPSTAT
                     NIQVTDTPSNLTITNVSGSGCLALPCTIASLASGANTQIFVTATINAAGAFDNSATAT
                     AAQPDPVPSNNTDNTLNGGTAVDSADLSIVKTFTTNAPYHHGDTITFNLHVTNNGPST
                     ATNVTVVDSNHPGLTITGVSGSGCSAVPCNIGTLASGASADVSVSATIDAPGAFQNNA
                     SVSADQNDPDSSNNSSQLNAVATTSADVSIVKAITTPGPYYAGESITYTLTVHDNGAS
                     AATMVTVTDTPTNMTITSASSNDNAGNHHCDSFPCTFGSPLANTHTVIITVTATITAD
                     GAFSNTATANGQDFDPDTSNNTSTAGGTAGPTADLAVVKTFITASPYHHGDTIQMNLH
                     VTNNGPSTATNIVVFDNGVGLSPVSMTGGNCTGASIGGNQNQNIQCTIASLASGASAD
                     VVVTSTINAPGAFQNNASVSTNDQIDPDSSNNSSQLNAIATTSADISVVKSITTPGPY
                     YAGETITYTLTVHDNGASASTQLTVTDTPSNMTIVSASSNDNAGNHHCDSLPCTFGSP
                     LANTHTVIITVTATINAAGPFSNTATVSGNDFDPNTANNTSTAGGTALASADVSLVKT
                     LVTTGPFTVGETITYTLVVANAGPSTATNIQVTDTPTNLTITNVSGGGCVALPCTIAS
                     LASGANTTITVTATIVTGGAFNNSATATGTESDPNPGNNTDNTGNGGSTAVADVSIVK
                     TLVTAGPYYAGQSVSYTLVIHNAGPSIATGIQVTDTPTNQTITGVTGSGCSALPCTIP
                     SLAVSASTTINVTATITAAGTFTNSATATPIEFDPNMSDNTGAAGNGGVALASADVSL
                     VKTLVTVGPFAPGQSISYTLVVHNSGPSTATSIQVTDTPTNLTITNVSGGGCAALPCT
                     IATLAVGASTTINVTATIVAAGAFDNSATATPAEFDPDTTNNTDNTGNGGSAAQAPAG
                     GPTLSTLGLIIMGLLLAMAGAMVMKIGE"
     gene            3819..4604
                     /locus_tag="VNN25_02815"
     CDS             3819..4604
                     /locus_tag="VNN25_02815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXH90487.1"
                     /translation="MLKAIAFDLWETLLTDTPEISRRQERLRLDRMERVLVERGFEET
                     AARIETAYRSFWHRCYERYWSRDEDIACRVQIEHFLDELGLDTATFDEPSLAALEDVY
                     ARAAVDILPSPVPGANEVLASLRERGYLIGLISNTGRTPGYALREILEHLGMASSIDA
                     MVFSNEHGVCKPNPSIFSTLRDSLGVDYDEMMFVGDNIYVDVHGAQSLGMTGVHFDPP
                     TRGTAIAPPVDHGLEIVPHATIRDLRELIEIVELHQGSVLAAR"
     gene            4794..5420
                     /locus_tag="VNN25_02820"
     CDS             4794..5420
                     /locus_tag="VNN25_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH90488.1"
                     /translation="MRYDNFIVPNHRNLNRRSRGRLPHWELDQANYSITFRLRDSLPR
                     AVAQALFEEREHILRTAMNASERAEADRMFALRLDSHLDAGYGSCILREDRNAAIVAG
                     ALQHFDRTRYNLHAWCVMPNHVHAMFYLERGADLPEIMHSWKSYTSHEIGQGVIWQRE
                     YYDRIVRDARDFRDSRNYVLNNPAKAGLRGWKWVGYGSGGVGEEQPTG"
     gene            5420..6346
                     /locus_tag="VNN25_02825"
     CDS             5420..6346
                     /locus_tag="VNN25_02825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH90489.1"
                     /translation="MAARTPPSQPAGRQRSAGAKRAMGEWGRCHKARLLRMERWRPRR
                     LARRRPAAVTRAETPATLFSMRDRSADDWNALGEAEPFFAVLTDERFLRERMSEVDRE
                     AFFASGEADIAYLFVLIAQPDFAPKSALDFGCGVGRLTRALTKRIERVAGVDVAPSML
                     RIARENVPQATFSAEIPDACFDLIVSLIVFQHIPVRRGEALLDELLSHLSDDGVAALQ
                     FTFRRPGSFLRRVARTIRGRVPLVHRIVLRMRGERPMPYMQMNEYDLERVTAILHRRG
                     CEEFRVIPTDHGGIKGAILVAKRKSGSVCPSN"
     gene            complement(6518..9427)
                     /locus_tag="VNN25_02830"
     CDS             complement(6518..9427)
                     /locus_tag="VNN25_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90490.1"
                     /translation="MKRNNRLIVLTALLAALQVSVLSLAAAETPKECALCAGVVSDLA
                     IVPATPVPLLVRIRQDDFAAAGTALDAMPPAVRAKMTIVIGYAVAADKDPMADVETHT
                     QAIVDWARTHGPFEGIGIAVDNVTPAVGGYAIKRLAVTAQGQNAASRIVFEGVGATPT
                     LTPESLTMLADTGAQSYVDVLLTDATSVKSTAAWVLEKDPVKKIWAVVPPQSPNVFFD
                     VARAFADGATRAFTSAPPTADLLASAASFDRALIGDYAFDSTAKIDLLDAKGNKIDEP
                     ALAFVRGEDLRTIIVPKGDASASTIVSLAGDDYKLPRRYDAAGDKDVTDVGTKGGRFL
                     MGMPPVTKPFVFTVEHNEVPRANVTKEALNITGHRGIPVEEIIRNHQTYKSYQESIQP
                     RYVARDATKLRFTMTGGDAIEATIAGDYFSDPKGRADWMWQDFYLNGVKWKYGKIPEL
                     PLIQPEKVTQLPLDIHLTNEYRYELVRETDLLGYHTYEVRFEPPAKAAATLPLYRGTV
                     WIDTRTWARIRLSMVQLNLTGEILSNDERVDFQPFARTTHTPLTAAEVAKADPREILW
                     LPKVVSAQQVVSAAGHGTVVLRETDFTDFRIDPADYDTHLAEAEKSDARMVRETKAGL
                     RYLERTPTGERVVKEGFDSSRTFLLGGIHHDSGLQYPVVPLGGIDYFNFDFKHTGIQT
                     NVFFAGVVLVANATNPNVANTQTNVGADLFAIAIPSTQSLYRGGKEQLGEEVKALPTR
                     ISIRAGHPFLQFGKVDVSLTVEHDTYMRSDNTASTFIVPSDTFVISPSIDGQYARWGA
                     TVTGFFDYNKRTTWKPWGNPAEFDPKQKTYMDFGGSIAKSIYLPKFQRIGVEVDYMDG
                     TRLDRFSEYGFGFFGAQRIHGIKSGSVLADRAILGHLSYGFVFSDQFRLEAFYDHGLI
                     DNKLAGYQREPFQGLGIAGQTVGPYGTLLRLDIGKTIGRNAQKGFVADVVFLKLF"
     gene            9491..10357
                     /locus_tag="VNN25_02835"
     CDS             9491..10357
                     /locus_tag="VNN25_02835"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXH90491.1"
                     /translation="MFFNPSSGLKMPAAEQDALQQAAAEAGLEVIRVTRGLDCADVIN
                     QRVQEGIRLFVAAGGDGTINAVVQPLVNTDAILGVIPVGTYNHFAKDLGIPLVWRDAL
                     DVIVSGATKPIDTARVNDRFFVNNVSMGLYPELVARREERGRDYPRWKARLFAALGTL
                     RKYPHIAVTLDTENHHEVIRTHVLMISNNVYDLSRLGIEAPRPALEEGRLSIYWLPHV
                     RRLALTSFVAHYLAGRVHSAPGFRSFRTSRVRVQSSKKHFSVGVDGEVVTMETPITIT
                     IVPQSLSVKVPR"
     gene            10452..11447
                     /locus_tag="VNN25_02840"
     CDS             10452..11447
                     /locus_tag="VNN25_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXH90492.1"
                     /translation="MKTHWCLFAVALFCFEAGAAIGADPANLVVNGDFEQNGGLGTSV
                     FTGWTVFNLTGSSGSWYAQMGTMPVPATQGCSKDFVVPQPPSGFAAMTTQSTHGSHIL
                     YQDVAIPSGASSVTLSFDLYLNSGSVFEIAPTLDFHQTPNQQFRVDLLDPSAPVADTG
                     SGVLANLFQTKATDSRFDTAYSRHTYDLSLFAGRTVRVRFAEVDSVDCFSAGVDNVSI
                     TAAACPASAPGSVVVAFHGGSTGCTTSATKCAAGEAISFSALGVGYTFQDCDTYSWDF
                     GDGGVSSLRSPNRAYASAGSYTVRLTVTNSLGSSAAGAVVVNVGPSPPRRRAAKP"
     gene            11724..13016
                     /gene="asnS"
                     /locus_tag="VNN25_02845"
     CDS             11724..13016
                     /gene="asnS"
                     /locus_tag="VNN25_02845"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HXH90493.1"
                     /translation="MTNVRIKELSQHVGEKVTIDGWLYNKRTAGKLIFPIVRDGSGYL
                     QCVVFKKEVSEETWKAADEVTQESSVRIIGTVRSEARAPGGVELGVETLEIVGPAADY
                     PISPKEHGTDFLMNHRHLWLRSSKQHAVLRIRNEVEKGIRDFFYDRDFVLIDSPILTA
                     NAAEGTSTLFETDYFGEKAYLSQSGQLYLEPAAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFLEFEGLQSLAEEFVEYLVTRALERCQEELKTLERDQSKLEGVKRPFP
                     RITYREAIDLLASKGMDAKFGDDLGGDEETVIANSFDRPVMISRYPTDIKAFYMQPDP
                     NDPSVVLGLDMIAPEGYGEIIGGSQRIHDHDLLLERIKQHKLPVEKFQWYLDVRKYGT
                     FPHSGFGMGIERCVAWISGVPHLRETIPYPRMLNRIYP"
     gene            13117..14817
                     /locus_tag="VNN25_02850"
     CDS             13117..14817
                     /locus_tag="VNN25_02850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXH90494.1"
                     /translation="MPEDTDNRQPTTDNAAPPGAALRIIPLGGLGEIGMNLMVYEYGD
                     DAIIADCGMMFPDASTLGVDVIVPDMTYLFDRPERVKAVFLTHGHEDHIGAVPFLVEQ
                     LHVPVYGMPLTLGFVRDKLEEFGIEDVDLRPFMPRDVVDAGVFRVEGIQVTHSIVDAL
                     GLAIRTPVGTLIHTGDFKIDHTPVDGKRTDLARFAAYGEEGVLALMSDSTNALVPGHG
                     PSEKSVGNSLVNIFANLTGRIIVTTFASHIHRVQQIVDAARKHKRKVFLIGRSLVDNA
                     ETAERLGYLRIPREQRPGANAKPSDYANHEVLILTTGTQGEPSSALSRMAIGEHKSVE
                     IQKGDVVILSARTIPGNERAVSHVIDNLYRRGAEVLNWENASVHVSGHACEEELKLML
                     NVTRPKFFIPIHGTLRHLIHHARLAKSVGVPHGIVITNGQVAEIAGDEIRVLEERVPY
                     GKVFIDSEAEEVPEVVVRDRKHLAEDGFVIVVVAIDSNGLVGREPEIITRGLLHVDES
                     QDILAEVRAQLVQMLHACGPDELRDSDLVQEKMRALLKRYFRKSMGRRPMILPVIWEM
                     "
     gene            14939..15763
                     /gene="uppP"
                     /locus_tag="VNN25_02855"
     CDS             14939..15763
                     /gene="uppP"
                     /locus_tag="VNN25_02855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HXH90495.1"
                     /translation="MTIPEAIILGLVQGLTEFIPVSSTAHLLIVPSVLGWGDPGAAVS
                     AVIQFGTLLAAIIYFFSDIVRLIGGFFRGLVTRRPLADVDSREAWLVVIGTIPIVILG
                     LLFKKHIESTFRGLWIVTTMVIAVAILMHIAEAYAKRRQFRGFDDMTVMDAAVIGLGQ
                     CLALIPGSSRSGSTIMPAIFRGIDRPTAARYSFLLSIPAVGGAGFLELFKERHALGAL
                     GWTPIAVAIVVAFISGYASIWFLIRYLRTHTTHVFIYYRYALGAAMIAMLMTGYLK"
     gene            complement(15923..15994)
                     /locus_tag="VNN25_02860"
                     /pseudo
     CDS             complement(15923..15994)
                     /locus_tag="VNN25_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
     gene            complement(16082..16600)
                     /gene="hpt"
                     /locus_tag="VNN25_02865"
     CDS             complement(16082..16600)
                     /gene="hpt"
                     /locus_tag="VNN25_02865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXH90496.1"
                     /translation="MAQTTLFTKADIDRRVAEMGAEITRDLAGSDLIAMCVLKGAMFF
                     CSDLMRQIAFDVALDFIQISSYGNQKTSSGVVTILKEPQLDMRDRTVLIIEDIIDSGL
                     SMKEVFRYIKLDRGAKEVRTATFLDKPTARKVEFKADYIGFSIDPKFVVGYGLDFAEK
                     YRNIPDIQVLEE"
     gene            16633..17388
                     /locus_tag="VNN25_02870"
     CDS             16633..17388
                     /locus_tag="VNN25_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXH90497.1"
                     /translation="MKIYRFLDGGRVAIGVEDGERIVRYTGRDATALGQATSHAIPRG
                     TAELLPPVVPTKIVAVGRNYAEHAKELGNEAPSEPIIFLKPPSALLPPNGTIIRPPQS
                     QRVDFEGELAIVIGKTARNITAANWRSVVLGFTCANDVTARDLQKKDVQFTRGKSFDT
                     FCPLGPCIETDLDPSALSLRTRVNGDVKQNGNTRDMVFNCGAVIEFITSVMTLEPGDV
                     ILTGTPAGVGPLAAGDHVEVEIEGIGVLQNVVA"
     gene            17456..18511
                     /locus_tag="VNN25_02875"
     CDS             17456..18511
                     /locus_tag="VNN25_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90498.1"
                     /translation="METLRADLLRLFPRLRTLPADAYVVGGAVRDLIFGAAPADVDVA
                     CLDPLACARVQGRNVIRLGREDHLSAWRVVDGPHVYDFAALLDGSIDADLARRDFTVN
                     AMAVDLRSGALLDPHGGRADLKARIVRMVDASNFDDDPLRMVKAVRMAVRFGFSIDDA
                     TLTAIRERAPRVADVAVERVAYELTVIFSAKSFRRALALLHETALDVPLFDRALDPNA
                     FEADDVPLAAALALLVPDAEGFAKRWRLSGTLLRDVVTLQHLSDAHSLLDLYDAGEIV
                     AGQLTPMLRAVGREERIDLPDFAMRPLLTGDEIASLAAIAAGPRVGQLKRALLEEQLS
                     GRITTREDAVAFITASA"
     gene            complement(18495..19166)
                     /locus_tag="VNN25_02880"
     CDS             complement(18495..19166)
                     /locus_tag="VNN25_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90499.1"
                     /translation="MRKQLLFVAAALLLAGAAAADDEVTHSFSTSSARAHVHRVVIDI
                     PAGSVKIRNGASDRIVVRGEVRRHFDGYRRRAKEQSIADDISTEIAIDGDEATVRRHY
                     GPGAQSWSAKSIHTEVDVTVDVPPGVDVDVSTRYGEVNIDGAFGRIDTDLRAGEVHVR
                     TPRVNVRDLRASVLIGEVHTYFGDHYIENEGVFPRTAHFYNAAGRSDVSVHSTFGEVH
                     VTLTQ"
     gene            complement(19356..19517)
                     /locus_tag="VNN25_02885"
     CDS             complement(19356..19517)
                     /locus_tag="VNN25_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90500.1"
                     /translation="MIATNWKNNLEAPVNVEGRRLFTPRRNPNPKNDCPEKSGVFCQV
                     GATVRSRTA"
     gene            complement(19632..20639)
                     /locus_tag="VNN25_02890"
     CDS             complement(19632..20639)
                     /locus_tag="VNN25_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXH90501.1"
                     /translation="MRIGIDARKIADFGIGTYIRGLLGALVAQRDGDTYVAFAPESHA
                     RFLPAGIEHVVLDAPHYSIRELVAVGRAADRAKIDLFHAPHYVVPFTSVPLVVTVHDL
                     IHLRHPNPLARMYARQMLGRAVRRSQRVLTVSESVKREIVATFGCADEHVVVTPNGVG
                     PPFAAEGNAAEGRYFLYVGNDKPHKNVDVLVDAFDRIDGASLVLTGAPFERFRDRERV
                     VVSGFVSDDELAALYRGAIALVMPSREEGFGLPALEAMACGCAVITSNAAALVEITGD
                     AALHAEPAIDALADAMQRVASDAALRADLARRGIERAKNFTWMRCAERTRGAYRASMT
                     R"
     gene            complement(20636..21655)
                     /locus_tag="VNN25_02895"
     CDS             complement(20636..21655)
                     /locus_tag="VNN25_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXH90502.1"
                     /translation="MNFQVTIDGRALVGNRTGIGVHTAEIARRLPDAPLIASHAEIAD
                     RSGLERCRFRVDPMALGVLWQQFVLPRIAKSGVVWGPHGTLPLALRIPSVATLHDFSS
                     LTMPFRHRAKTVLSFNAFIGRSLQMATRIAAVSRAVAEEAVRWFGVARERIEIVPNGV
                     DDFFSSAIEAGESDYVLYVGTIEPRKGLDDLLAVWDSLPQPRPRLVLCGDAGWGDVQL
                     PNGAEVTGYVDRTTLRDLYRGARAFVYPSRYEGFGIPPLEAMACGSPVIATRTGAIPE
                     YADGAALLIDPGDRNALRASLLQLLGDQTLRDELRSRGAERARAYSWERSAALMTQLL
                     AEAAR"
     gene            complement(21751..>22003)
                     /locus_tag="VNN25_02900"
     CDS             complement(21751..>22003)
                     /locus_tag="VNN25_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90503.1"
                     /translation="EAMAYGVPSIAARSSSLPEIGGDAALYFEPHDAKGLEAQISRVL
                     MDAALREHLAAAGPLRAAQFRWDVAAEQTLAVLRKAAGS"
BASE COUNT         4080 a         7170 c         6724 g         4029 t
ORIGIN      
        1 tcgctggtcg tcaatccgcc tagtccgtcg atcggactgc tcaaacaggt cggcaccagc
       61 aataccggac cgtggaattc attccttgcc atcgcctccg gccctgtctt ctatcgcttc
      121 accgttgaga acaccggcga cgttccgctc acgctcggcc cgacgacgct caccgacaac
      181 aacgtggatg tctcggcttg caacgcagga ttcaacggct tgatcctgcc cgtggctgtg
      241 gccgggaatg acaaccacat cgtcacctgc gtcgtcggac ccgttgcttc gtcgcccggc
      301 gcgcacacca acactgcgag cgccgccgct acgttcaacg gaaatccggt gacatcacca
      361 aacgactccg cgacgtacgc caccaccagc ctgaccctcg tcaagagtgc cacggagagc
      421 acgttcacgc tccccggcga cacgctgcac tacagctatc tcgtcacgaa cagtggcttc
      481 gcgccgctgc agggtccggt caccgtcgcc gacaacaaaa cgacggtgac atgtccacca
      541 gtcaacaccg tcggcgacct ggacaacttc ctcgatccgg gcgagtcgat cacctgcacg
      601 gcgacctata tcgtgacgcc ggcagacgta tcgaatgcgc aggtgacgaa cacggcgcag
      661 gcgacagctg acagcgtcaa ctcgaacagc tccagcaaga tggtgccact ctccacctcg
      721 gccgatgtgt cgatcgacaa gagtttaaca acggccgcac cgtacctcac cggccagacc
      781 attaactaca cgctcacggt ggccaacgcc ggcccatcga cagcgacaaa catccaggtc
      841 accgacacac cctcgaacct gacgatcaca aacgtgagcg gcagcggctg cttggcgctt
      901 ccctgcacga tcgccagtct cgcatccggc gcgaacacac agatcttcgt cactgccacg
      961 atcaatgccg ccggtgcatt cgacaacagc gccactgcca cggccgcgca gcccgatcct
     1021 gttccgtcga acaacaccga caacacgctc aatggcggta cggccgtcga ttcagccgac
     1081 ctctccatcg tcaagacctt caccaccaac gccccgtacc atcacggcga caccatcacg
     1141 ttcaatctcc acgtgaccaa caacggcccg tcgacggcga cgaacgtcac cgtcgtcgac
     1201 agcaaccatc ccggcctgac cattaccggc gtcagcggca gcggctgttc cgcggtgccg
     1261 tgcaacatcg gcacgctggc cagcggagca agtgccgacg tcagcgtctc cgcgaccatc
     1321 gatgctccgg gcgcgttcca aaacaacgcc agtgtctccg ccgaccagaa cgatcccgac
     1381 tcgtcgaaca acagcagcca gttgaacgcc gtggccacga cttcggcgga cgtctccatc
     1441 gtgaaggcca tcaccacgcc cggcccgtac tatgccggcg agtcgatcac atacacgctg
     1501 accgtccacg acaacggcgc ctcggccgcc acgatggtca ccgtcaccga tacgccgacg
     1561 aacatgacca tcaccagcgc cagcagcaac gacaacgccg gaaaccatca ctgcgattcg
     1621 ttcccctgca cgttcggctc gccgctggcc aatacccata ccgtcatcat caccgtcact
     1681 gccacgatta ctgccgacgg cgccttcagc aacaccgcca ccgccaacgg ccaggacttc
     1741 gatcccgaca cgtcgaacaa cacatccacc gcaggcggga ccgctggccc gacggccgac
     1801 ctcgcggtcg tgaagacctt catcaccgct tcgccctacc accacggcga cacgattcag
     1861 atgaacctgc acgtgacgaa caacgggccg tccaccgcca cgaacatcgt ggtgttcgac
     1921 aacggcgtcg gcctctcccc ggtgagcatg acgggcggca actgcaccgg cgcatcgatc
     1981 ggcggcaacc agaaccagaa cattcagtgc acgatcgcct cgctggcatc aggagcaagc
     2041 gccgacgtcg tggtgacctc gacgatcaac gcccccggcg ccttccagaa caacgccagc
     2101 gtcagcacga acgatcagat cgatccggat tcgtcgaaca attccagcca gttgaacgcc
     2161 atcgccacca cctcggcgga catctccgtg gtgaagtcga tcaccacgcc tggcccgtac
     2221 tacgccggcg agacgatcac gtacacgctc acggtccacg ataacggcgc ctcggcctcg
     2281 acgcagttga ccgtcaccga cacgccgtcg aacatgacca tcgtcagcgc cagcagcaac
     2341 gacaacgccg gcaatcatca ttgcgactcc ttaccctgca ccttcggctc gccgctggcc
     2401 aacacccaca ctgtgatcat caccgtcacc gccacgatca acgccgccgg cccgttcagc
     2461 aacaccgcca ccgtcagcgg caatgacttc gatcccaaca ccgcgaacaa cacgagcact
     2521 gcaggcggca cggccctcgc gtccgcagac gtatcgctcg tgaagacgct cgtcacaacc
     2581 ggcccgttca cggtcgggga gacgattacc tacacgctcg tcgttgccaa cgccggaccg
     2641 tccaccgcga cgaacattca ggtcacggac accccgacga acctgacgat cacgaacgtc
     2701 tcgggcggcg gttgcgtggc cctgccgtgc accattgcca gccttgcctc gggcgccaat
     2761 acgacgatca cggttactgc caccatcgtt acgggcggag cattcaacaa cagcgccacg
     2821 gccaccggaa ccgaatccga tccgaacccg ggcaacaaca ccgacaacac cggcaacggc
     2881 ggatcgaccg cggtggccga cgtttcgatc gtcaagactc tcgtgaccgc cgggccgtac
     2941 tacgccggac aaagcgtcag ctacacgcta gtcattcata acgccggccc gtcaatcgcc
     3001 accggcattc aggtgaccga cacaccgacg aaccagacca tcaccggcgt cacgggtagc
     3061 ggctgttcgg cgctgccgtg cacgatccca agcctcgcag tgagcgccag cacgacgatc
     3121 aacgtcacgg ccacgatcac tgcggccggc acgttcacca acagtgcaac cgcgacgccg
     3181 atcgagttcg atccgaacat gtccgacaac acaggagccg cgggcaacgg cggcgtggct
     3241 ctggcctcgg cggacgtctc cctcgtcaag acgctcgtca ccgtagggcc gttcgcgccc
     3301 ggtcagtcga tcagctacac gcttgtcgta cataactccg gtccctcgac ggccacgagc
     3361 atccaggtca ccgacacgcc aacgaacctg acgatcacca acgtctcggg tggtggttgc
     3421 gcggcccttc cgtgcacgat cgcaaccctt gccgtcggtg ccagcacaac gatcaacgtc
     3481 acagccacga ttgtggccgc gggcgcgttc gacaacagcg cgaccgcgac gccggcggaa
     3541 ttcgatccgg acaccacgaa caacaccgac aacaccggca acggaggcag tgcggcgcaa
     3601 gcccccgccg gtggcccgac cctgagcacg ctcggcctca tcatcatggg cctgctgctg
     3661 gccatggccg gcgcaatggt catgaagatc ggcgaataac gacaggggag tgagggcaga
     3721 cgcgcgtctg tcctcactcc gtttctgtca cgccggtccg gccaaagcgc gtggaaaacg
     3781 cggggtagaa tcgtccggca tcgcttcgtc cacattccat gctcaaagca atcgccttcg
     3841 atctctggga aacgctgctc accgacacgc ccgaaatctc gcggcgtcag gagcggctgc
     3901 ggctggacag aatggagcgc gttctcgtcg agcgcggatt cgaagagacg gccgcgcgca
     3961 tcgaaactgc gtaccgaagt ttctggcatc gatgctacga gcgctactgg tcgcgcgatg
     4021 aagacatcgc ttgccgtgtg cagatcgagc actttctcga cgagctggga ctcgatacag
     4081 caacgttcga tgaaccgtcg ctggccgctc tcgaagatgt ctacgcccgc gccgccgtcg
     4141 atatccttcc tagtcctgtc ccaggcgcga acgaggtgct ggcatcgctg cgagagcgcg
     4201 gatatctcat cggtctgatc agcaataccg gccgcacacc cggctatgcg ctccgcgaga
     4261 ttctcgagca cctcggcatg gcctcgtcga tcgatgccat ggttttctcg aacgagcacg
     4321 gcgtctgcaa accaaacccc tcgatcttct ccacccttcg cgactcactc ggcgtcgatt
     4381 atgacgagat gatgttcgtc ggcgacaaca tctacgtcga cgtccacggc gcccaaagcc
     4441 tcggcatgac cggcgtccac ttcgaccccc ccacgcgcgg caccgccatc gccccgcccg
     4501 tcgatcacgg cctggagatc gtgccccacg caacgatcag ggatttgcgc gaactgatcg
     4561 agatcgtcga gttgcatcag ggttcagtgc tcgcggcgcg gtaggcggcg aggacgccgc
     4621 ctggccagcc ggcgggacgc cagcgttccg ccggcacaaa gctcgacacc tgcggatgga
     4681 acgctggcgt cctcgccggc tggctcggcg gcgtcccgcc gccgttgcat tgccacccac
     4741 tccgaggtag ccttctcgcc ccacaccatc agacgcgctg gctccggcgc gctatgcgct
     4801 atgacaattt cattgtcccc aatcacagaa atctaaatcg ccgttcaagg ggaagactcc
     4861 cgcactggga gctggatcag gcgaactact caattacctt ccgccttcgc gactcgctgc
     4921 cacgggccgt cgcccaggca ctattcgagg agcgtgagca catccttcga acagccatga
     4981 atgcctcgga gcgcgcagaa gcggaccgca tgttcgcctt gcggctcgat tcgcatctcg
     5041 acgcgggcta cggaagctgc atcctgcgcg aggatcgaaa tgctgccatc gtcgcgggtg
     5101 ccttgcagca cttcgatcgg actcggtaca accttcacgc gtggtgcgtg atgccgaatc
     5161 acgttcacgc gatgttctac ctcgagcgcg gcgccgatct gcccgagatc atgcattcat
     5221 ggaagtcgta cacgtcgcat gagatcgggc aaggcgtgat ctggcagcgc gagtactacg
     5281 atcgcatcgt gcgcgacgcg cgagactttc gggacagccg gaactacgtg ctgaacaacc
     5341 ctgcgaaagc gggattgcgc gggtggaagt gggtgggcta cggcagtggc ggagtaggcg
     5401 aggagcagcc gaccgggtga tggcggcgag gacgccgcca agccagccgg cgggacgcca
     5461 gcgttccgcc ggcgcaaaac gcgcgatggg cgaatgggga cgctgccaca aagcgcgtct
     5521 cttgcggatg gaacgctggc gtcctcgccg gctggctcgg cggcgtcccg ccgccgtcac
     5581 ccgcgcggag actcctgcta cgctcttctc gatgcgcgac cgttccgctg acgactggaa
     5641 tgctctgggt gaggcggagc cgttcttcgc cgtccttacc gacgagcgct ttcttcgcga
     5701 acggatgtcc gaggtggatc gggaggcttt cttcgccagc ggggaggcgg acatcgccta
     5761 ccttttcgtt ctcattgccc aacccgactt tgcgccaaag tccgcgctcg actttggatg
     5821 cggtgttggg cgcctgacgc gcgcgttgac gaagcggatc gagcgtgtgg ccggcgtcga
     5881 tgttgcgccg agcatgctac ggatcgctcg tgagaacgtt ccgcaagcga cgttttcggc
     5941 ggagatcccc gacgcctgct tcgacctcat cgtctcgctg atcgtttttc agcacatccc
     6001 cgtgcggcgg ggcgaggcgc tgctcgacga gcttttaagc cacctctccg atgacggcgt
     6061 agctgcgctg caattcacct tccgccgtcc aggctcattc ctgcgccgag tagcacgcac
     6121 gattcgaggc cgtgtgccgc tcgtgcatcg catcgtcctg cgcatgcgcg gcgagcggcc
     6181 gatgccctac atgcagatga atgagtacga cttggagcgc gtgaccgcga tccttcatag
     6241 gcgcggctgc gaggaattcc gcgtcattcc gaccgatcat ggcgggatca aaggggcaat
     6301 tttggtcgca aagcggaaaa gtgggagcgt gtgtccgtct aactgaatgc gtgcagaggc
     6361 cccctcgatg gccaaggttg ggcacggcta cggctggtgc gagcggcggc cctgcacggt
     6421 tttgttttgg ggagtgggct gtggggggtg gggtgtggga gagagacagc gttcttgaaa
     6481 ctctcccaca ccccactccc tacaccccgc cccccgatca aaacaacttc aagaacacca
     6541 catccgccac gaaccccttt tgcgcgttgc gtccgatcgt cttgccgatg tcgaggcgca
     6601 gcagcgttcc gtaggggcca accgtctggc cggcgatgcc gaggccctgg aacggctcgc
     6661 gctgatagcc ggcgagcttg ttgtcgatca ggccgtggtc gtagaacgct tccaggcgga
     6721 actgatcgga gaagacgaag ccgtacgaaa ggtggccgag gatggcgcgg tcggcgagga
     6781 cggagccgga cttgatgccg tgaatccgct gcgccccgaa gaagccgaaa ccgtactccg
     6841 aaaaacgatc gaggcgcgtt ccgtccatgt aatcgacttc cacgccgatg cgctggaact
     6901 tcggcagata gatgctcttg gcgatcgagc cgccgaagtc catgtacgtc ttctgcttcg
     6961 gatcgaactc cgccgggttc ccccacggct tccacgtcgt tcgcttgttg tagtcgaaga
     7021 agccggtgac cgtggcgccc cagcgtgcgt actgcccgtc gatcgacggc gagatgacga
     7081 acgtatcgga cggcacgatg aacgtcgatg cggtgttgtc gctgcgcatg taggtgtcgt
     7141 gttcgacggt cagcgagaca tcgaccttgc cgaactgcag gaacggatgc ccggcgcgga
     7201 tggagatgcg cgtcggcagc gccttcacct cttcaccgag ctgctccttg ccgccccgat
     7261 acagcgattg agtcgaaggg atagcgatcg cgaaaaggtc ggcgccgacg ttcgtctgcg
     7321 tgttggccac gttcggattc gtggcgttgg cgacgaggac gacgccggcg aagaaaacgt
     7381 tcgtctggat gcccgtgtgc ttgaaatcga agttgaagta gtcgatgccg ccgaggggga
     7441 cgaccggata ctgcaggccg gagtcgtggt gaatgccgcc gaggaggaag gtgcgcgagg
     7501 agtcgaagcc ttctttgaca acgcgctcgc cggtcggtgt gcgttcgaga tagcggaggc
     7561 ccgcttttgt ctcgcgcacc atccgcgcgt ccgacttctc ggcttccgcg aggtgcgtgt
     7621 cgtagtcagc gggatcgatg cgaaagtctg tgaagtcggt ctcacgcaga acgacggtgc
     7681 cgtgtcccgc cgccgagacg acctgttggg cgctgacgac tttcggcagc cagagaatct
     7741 cgcgcgggtc ggccttggca acttccgcgg ctgtgagcgg cgtgtgcgtc gtgcgcgcga
     7801 acggctggaa gtcgacccgt tcatcgttcg agagaatctc gccggtcagg ttgagctgca
     7861 ccatcgacag gcggatgcgc gcccacgttc gggtgtcgat ccacaccgtg ccgcgataga
     7921 gcggcagggt cgccgcggcc ttcgctggcg gttcaaagcg cacttcgtag gtgtgatagc
     7981 cgaggaggtc ggtctcgcga acgagctcgt agcgatattc gttcgtgaga tggatgtcga
     8041 gcggaagctg cgtcaccttc tcgggttgga tcagcggcag ctccgggatc ttcccgtact
     8101 tccacttcac gccgttgagg tagaagtcct gccacatcca gtcggcgcgc cctttcggat
     8161 cggagaagta gtcgccggcg atggtcgctt cgatcgcatc gccgcccgtc atcgtgaagc
     8221 gaagcttggt ggcgtcgcgc gcgacgtagc ggggctggat cgactcctgg tatgacttgt
     8281 acgtctggtg attgcggatg atctcttcga ccgggatgcc gcggtggccc gtgatgttca
     8341 acgcttcctt ggtcacgttc gcgcgtggca cttcgttgtg ctcgaccgtg aagacgaacg
     8401 gcttcgtcac gggcggcatc cccatcagga aacggccacc ttttgtgccg acgtcggtca
     8461 cgtctttgtc gcccgccgcg tcgtagcggc ggggaagttt gtagtcgtcg ccagccagcg
     8521 aaacgatggt cgacgcagaa gcatcgccct tcggaacgat gatggtgcgg agatcctcgc
     8581 cgcgcacaaa cgccagggcg ggctcgtcga tcttgttgcc tttggcgtcg agcagatcga
     8641 tcttggccgt ggagtcgaag gcgtagtcgc cgatgagtgc gcggtcgaaa ctggccgccg
     8701 aggcgaggag atcggcggtt ggaggagcgc tcgtgaaggc acgggtcgcg ccgtcggcga
     8761 acgcgcgggc gacatcgaag aaaacgttcg gcgattgcgg cggaacaacg gcccagatct
     8821 tcttcaccgg atccttttca agaacccacg ccgcggtcga cttcacgctc gtggcatcgg
     8881 tgaggagtac gtcgacgtac gactgcgcgc cggtgtcggc gagcatggtg agcgattcgg
     8941 gagtcagcgt cggtgttgct ccgactccct cgaacacgat cctcgacgcc gcgttctgcc
     9001 cttgtgcggt aacggcgagc cgtttgatcg catagccgcc gacggccggc gtcacgttgt
     9061 ccacagcgat gccgattccc tcgaatggtc cgtgtgtgcg cgcccagtcg acgatcgcct
     9121 gcgtgtgcgt ctcgacgtcg gccatcgggt ctttgtccgc agcaacggca tatccgatga
     9181 cgatggtcat cttcgcgcgc acggctggcg gcatggcatc gagcgcggtg cccgctgccg
     9241 cgaaatcatc ctggcggatg cgaacgagca gcggcacggg tgtggcaggg acgatcgcga
     9301 ggtccgaaac cacaccggca cagagagcgc attctttcgg agtctcagca gctgccagcg
     9361 agaggacgga aacttgcagt gcagcgagga gggcggtcag gacgatgagt cgattgttcc
     9421 gtttcatttt gccgttctgc aaacgccagc gttggagttg atattgccgg agcaaaaagt
     9481 taaaggcgtc atgtttttca atccgtcatc gggcctgaag atgcctgcgg cggagcaaga
     9541 tgctctccag caggcggccg ccgaggccgg actggaggtc atccgcgtga ctcgcgggct
     9601 cgattgtgca gatgtcatca accagcgagt gcaggaaggg attcgcctct tcgttgctgc
     9661 gggaggcgac ggaacgatca acgcggtcgt tcagccactc gtgaacacgg atgcaatcct
     9721 cggcgtgatt cccgtcggca cctacaacca tttcgcgaaa gacctcggca tcccactcgt
     9781 ctggcgcgac gcgctcgatg tgatcgtctc gggtgccacg aagccgatcg ataccgcccg
     9841 cgtgaacgat cgcttcttcg tcaacaacgt ctcgatgggt ctctatcccg aactggtggc
     9901 gcgccgcgaa gaaaggggac gcgattatcc gcgctggaag gcccgtcttt tcgccgcgct
     9961 tggcacgctc cggaagtacc cgcacatcgc cgtcacgctc gatacggaga atcaccacga
    10021 ggtgattcgc acgcacgttc tgatgatctc gaacaacgtg tacgacctct cgcgcctcgg
    10081 catcgaagcg ccgcgcccgg cactcgagga agggcggctg tcgatctact ggttgccgca
    10141 tgtccggcgc ctcgcgctga ccagtttcgt ggcccactat ctcgccggcc gcgtacatag
    10201 cgcaccaggc ttccgctcgt tccgtacgtc gcgcgtgcgc gtgcagtcgt caaagaaaca
    10261 cttctccgtc ggcgtcgacg gcgaagtcgt gacgatggaa acgccgatca cgatcacgat
    10321 cgtgccgcag tcgctgtcgg tgaaagtgcc gcgctgacgg cggcgagacg ccggcgctcc
    10381 gtcaagcccc gcaccacctg aatccagtcc ggttgtttgc tcgtagtaac tggcaaggag
    10441 aatcggatgc catgaaaacg cattggtgcc tcttcgccgt tgctcttttc tgcttcgaag
    10501 ccggcgctgc catcggtgcc gatccggcga atcttgtagt gaacggcgac ttcgagcaga
    10561 acggagggct cggcacaagc gtatttaccg gttggacagt gttcaatctg acgggaagtt
    10621 ccgggagctg gtatgcccag atgggaacca tgcctgtgcc ggccacacag ggctgctcga
    10681 aggatttcgt cgttccccag ccgcccagcg ggttcgccgc gatgaccacg cagagcactc
    10741 acggctcgca catcctctat caggacgtcg cgattcccag cggagccagc tccgtgacgc
    10801 tgtcattcga cctctacttg aactcgggct ccgttttcga gatagcgccg acgcttgact
    10861 ttcaccagac gccgaaccag caatttcgag tcgatctgct ggatccgtcg gcgccggtgg
    10921 cggataccgg ctcaggagtg ctggccaatc tcttccaaac caaggccacg gactcgcgct
    10981 tcgacacggc atactcacgt catacatacg acctctcgtt gttcgccgga cgtacggttc
    11041 gagttcgctt cgcggaagtc gacagcgtgg attgtttcag cgcaggagtc gacaatgtct
    11101 cgatcacggc cgctgcctgt ccggcaagcg ctccggggtc cgtcgtcgtc gcgtttcacg
    11161 gcggttcaac cggctgcact acctcggcaa ccaaatgcgc agccggggaa gcaatctcct
    11221 tttcggcgct cggggtcggc tacacgttcc aggactgcga cacctactca tgggacttcg
    11281 gcgacggcgg tgtttcatca ctccgctccc ccaaccgcgc gtatgcatcg gcaggctcct
    11341 acaccgtgcg gttgacggtc accaattcgc tcggttccag tgctgccggc gctgtcgtcg
    11401 tgaacgtcgg accttcgccc ccgcgtcgga gggcggccaa gccttagccg ggcgcttcca
    11461 cctcggcgtc gatggcgact cgtcacgctc gggccgcaat cgctgtcggt gaaggcgacg
    11521 cggtgacggc ggcgagacgc cgccgagcca gccggcgagg acgccagcgt tccatccgcg
    11581 tatggcgcgc tgagcaacgc tcaaacccgc acatcgcgcg ccttgtgccg gcggaacgcc
    11641 ggcgtcccgc cggctggcca ggcggcgtct cgccgcctaa cgcgcaggac gccggacttc
    11701 gtccgctata atcccggccc tttatgacca acgttcgaat caaagagcta tcgcagcatg
    11761 tcggggagaa ggtcaccatt gatgggtggc tgtacaacaa gcgcacggcc ggcaagctga
    11821 ttttccccat cgtccgcgac ggcagcggct atctgcagtg cgtcgttttc aagaaggaag
    11881 tcagcgaaga gacgtggaag gctgccgacg aagtgacgca ggagtcgtcg gtgcgcatca
    11941 tcggtacggt tcgcagcgag gcgcgtgctc ccggcggtgt ggagctcggc gtcgaaacgc
    12001 tggagatcgt cggcccggct gctgactatc cgatctcgcc gaaagagcac ggcaccgact
    12061 tcctgatgaa ccaccgccac ctctggctgc ggtcatcgaa gcagcatgcc gttctgcgca
    12121 ttcgtaacga ggtcgagaaa ggcatccgcg atttcttcta cgaccgcgat ttcgtcctca
    12181 tcgattcgcc gatcctcacc gccaatgctg ccgagggaac gtcgacgctg ttcgagaccg
    12241 actacttcgg cgagaaggcc tacctctcgc agtcgggcca gctctatctc gagccggcag
    12301 cggcagcatt cggcaaggtc tactgcttcg gtcctacctt ccgcgccgaa aaatcgaaaa
    12361 cgcgccgcca tctgatggag ttctggatgg tcgagcccga ggttgcgttc ctcgagttcg
    12421 aagggctgca gtcgctggcc gaggagttcg tcgagtacct cgtcacgcgc gcgctcgagc
    12481 gctgccagga agagttgaag acactcgagc gcgatcagtc gaagctcgaa ggggtgaagc
    12541 ggccgttccc acgcatcacc tatcgcgaag ccatcgatct tctcgcctcc aaagggatgg
    12601 atgccaaatt cggcgacgac ctcggcggcg acgaagagac agtcatcgcc aactccttcg
    12661 atcgtcccgt gatgatcagc cgctacccga ccgatatcaa agcgttttac atgcagcccg
    12721 atccgaacga tccctcggtc gttctcggcc tcgacatgat cgcgcccgaa ggctacggcg
    12781 agatcatcgg cggctcgcag cgcatccacg atcatgacct gctgctcgaa cggatcaagc
    12841 agcacaaact gccggtcgag aagttccagt ggtacctcga cgtgcgcaaa tacggcacct
    12901 tcccgcactc cggcttcggc atgggaatcg agcgctgcgt cgcctggatc agcggcgtcc
    12961 cgcatcttcg cgagacgatt ccgtatccgc gaatgctgaa tcggatttat ccctgattgc
    13021 gccttcggcg cgttgtcggt tgccggttgc cggttgtcag ttgtcagtta tccgttatcg
    13081 gttgtcggtg cgatagcatc gccgtcctca tgaaccatgc ctgaagatac cgacaaccga
    13141 caaccgacaa ccgacaacgc ggcgccgcca ggcgccgcac tcagaatcat ccctctcggc
    13201 gggctcggcg agatcgggat gaacctgatg gtctacgagt atggcgacga cgccatcatc
    13261 gccgactgcg ggatgatgtt tcccgacgcc tcgaccctcg gcgtcgacgt catcgtcccg
    13321 gacatgacct acctcttcga ccggccggag cgcgtgaagg cggtctttct cacgcacggc
    13381 cacgaagacc acatcggcgc ggttccgttt ctcgtcgagc aactgcacgt gccggtgtac
    13441 ggaatgccgc tcacgctcgg attcgtacgc gacaagctgg aagagttcgg gatcgaggac
    13501 gtcgatctcc gcccgttcat gccgcgcgat gtcgtcgacg ccggcgtgtt ccgcgtcgaa
    13561 gggatccagg tgacgcactc gatcgtcgac gccctcggcc tggccattcg cacgccggtg
    13621 ggaactctga ttcacaccgg cgacttcaag atcgatcaca ccccggtcga cggcaaacgc
    13681 accgatctgg cacgcttcgc ggcgtatggc gaagagggcg tgctggcatt gatgagcgat
    13741 tcgaccaacg cgcttgtgcc gggtcacggg ccgtcggaga agtcggtcgg caattcgctc
    13801 gtcaacatct tcgcgaacct taccggccgc atcatcgtca cgacgttcgc ctcgcacatc
    13861 caccgcgtgc agcagatcgt agacgccgcg cgcaaacaca aacgcaaggt ctttctcatc
    13921 ggacgctcgc tcgtcgacaa cgccgaaacc gccgagcgtc tcggctactt gcgcatcccg
    13981 cgggagcagc gcccgggagc gaacgcgaag ccgtccgact atgcgaatca cgaagtgctc
    14041 atcctgacga ccggcacgca gggcgagccg tcatcggctc tctcccgcat ggcaatcggc
    14101 gagcacaaat cagtcgagat ccagaagggc gatgtggtca ttctttcggc gcgcacgatt
    14161 ccgggcaacg agcgcgccgt ctcgcatgtc atcgacaacc tttaccgccg cggcgccgag
    14221 gtgctgaact gggagaacgc gagcgttcac gtctccggcc acgcctgcga agaagagctg
    14281 aagctgatgc tcaacgtcac gcgcccgaag ttcttcatcc ccatccatgg cacgcttcgg
    14341 catctcatac atcacgcgcg attggcgaag agcgtcggcg tcccgcacgg catcgtcatc
    14401 acaaacgggc aggtcgcgga gatcgccggc gacgagatcc gcgtgctcga agagcgcgtg
    14461 ccgtacggca aggtcttcat cgactccgag gcggaggaag tgccggaagt cgtcgtgcgc
    14521 gaccggaagc atctggccga ggatggcttc gtgatcgtcg tcgtggcgat cgactcgaat
    14581 ggcctggtcg gacgcgaacc ggagatcatc acgcgcggat tactgcatgt cgatgagagc
    14641 caggacatcc tggccgaggt gcgcgcacaa ctcgtgcaga tgctgcacgc gtgcggcccc
    14701 gacgaattgc gcgacagcga tctggtgcag gaaaagatgc gcgcgctgct caaacgctac
    14761 ttccgcaaat cgatgggacg aagaccgatg attctgcccg tgatctggga gatgtgaggt
    14821 gaagcagaga tcagggacta gagatcagag atcagagcgt gtaacgatcg gtccgatccg
    14881 tctgatcgct gatctctagt ctctcagcac tatcgagcac ccacggagaa cgaaaccaat
    14941 gaccatcccc gaagcaatca tcctcggcct cgttcagggc ctgaccgaat tcatccccgt
    15001 cagctcgacg gcgcatctgc tgatcgttcc gtccgttctt ggatggggcg atccgggggc
    15061 ggcggtgtcg gcggtgattc agttcgggac gctgctggcg gcgatcatct atttcttcag
    15121 cgacatcgtt cggctcatcg gcggcttctt tcgcgggctc gtcacgcgcc ggccgctcgc
    15181 ggatgtcgac tcgcgcgagg cctggctggt cgtcatcggc acgatcccca tcgtcatcct
    15241 ggggttgctc ttcaagaagc acatcgaatc gacctttcgc gggttgtgga tcgtcacgac
    15301 gatggtgatc gcggtggcca tcctgatgca catcgccgag gcgtacgcga aacggaggca
    15361 gttccgagga ttcgatgaca tgaccgtgat ggacgccgct gtcatcgggc tcgggcaatg
    15421 tctggcgctc atcccaggct cgtcacgcag cggctcgacg atcatgccgg cgatctttcg
    15481 cgggatcgac cggcccactg cggcgcgtta ctcgttcctg ctctcgattc cggcggtcgg
    15541 cggcgcgggc ttcctcgagc tcttcaagga gcgccacgcc ctcggcgcgt tgggctggac
    15601 gccgatcgca gtggccatcg tcgtggcttt catctccggt tatgcctcga tctggtttct
    15661 gatccgctac ctgcgaacgc acacgacgca cgtcttcatc tattaccggt acgcgttggg
    15721 ggcggcgatg atcgcgatgc tgatgacggg atacttgaag taggtaaact cactcttcta
    15781 aaacctgtat gtccgggaat gttccggtac ttctccgcga agtcgagtcc gtagctggct
    15841 acttacatta ggttttacgc aggtcggact ctccagtccg actactcgtg atgtcggact
    15901 agagagtccg acctacgtaa actcactctt tgagcgcctg aatctccggg atgttccggt
    15961 acttctccgc gaagtcgagt ccgtagccga cgacgggata cttacactag gttttacgta
    16021 ggtcggactc tccagtccga ccgcacgtga tgtcggacta gagagtccga cctacgtaaa
    16081 ctcactcttc taaaacctgt atgtccggga tgttccggta cttctccgcg aagtcgagtc
    16141 cgtagccgac gacgaacttc gggtcgatgg agaaaccgat gtagtctgct ttgaactcga
    16201 cttttcgcgc ggtcggcttg tcgaggaacg tcgccgtgcg cacttccttc gcgccgcggt
    16261 cgagtttgat gtagcggaag acttccttca tcgagaggcc gctgtcgatg atgtcttcga
    16321 tgatcagcac cgtccggtcg cgcatgtcga gctgcggttc cttcaggatc gtgaccacgc
    16381 cggacgacgt cttctgattg ccgtacgacg agatctggat gaagtcgaga gcgacgtcga
    16441 aggcgatctg ccgcatcagg tcgctgcaga agaacatcgc gcccttgagc acgcacattg
    16501 cgatgaggtc actgcccgcg aggtccctcg tgatctccgc gcccatttcc gctacacgcc
    16561 ggtcgatgtc agctttggtg aacaacgtcg tttgcgccat ggttgcggat tctacgatta
    16621 caattcgtcg ctgtgaaaat ctatcgcttt ctcgacgggg gccgtgtcgc catcggtgtg
    16681 gaggatggtg aacggatcgt tcgttacacg ggccgtgatg cgaccgcgct tggccaggcc
    16741 acaagccacg cgattccccg gggcactgcc gaactcctgc ctcccgttgt ccccacaaag
    16801 attgtcgcgg tgggacggaa ctacgccgag cacgcgaagg agctcggcaa cgaagcgcct
    16861 tccgagccga tcatttttct caaaccgccg agcgctcttc tgccgccgaa tggcacgatc
    16921 atccgtcccc cgcaatcgca gcgcgtcgat ttcgagggag agctggccat cgttatcggc
    16981 aagaccgcgc ggaacatcac cgccgccaac tggcgcagcg tcgtcctcgg cttcacctgc
    17041 gccaacgacg taaccgcccg cgatctgcag aagaaggatg tgcaattcac gcgcggcaag
    17101 agcttcgaca ccttctgccc cctcggcccc tgcatcgaaa cggatctcga tccatccgcg
    17161 ctctcgcttc gcacgcgggt caacggtgat gtcaaacaga acggcaacac gcgcgacatg
    17221 gtcttcaact gcggcgccgt catcgagttc atcacctcgg tgatgacgct cgaaccaggc
    17281 gacgtgatcc tgaccggcac tcccgccggc gtcggccccc tcgccgccgg cgaccacgtc
    17341 gaggtcgaga tcgaaggcat cggggtcttg cagaatgtgg tcgcctagga gtgcgggcgt
    17401 ctcgcccgcg gccatcgggc gtcccgcccg atggtgttag ttggggcatg acgccttgga
    17461 aaccctccgc gccgacctcc tccgcctctt cccgcgcctt cgcacgcttc ccgcggatgc
    17521 ctacgtcgtc ggtggggcgg ttcgcgatct gatcttcggc gctgctcccg ccgacgttga
    17581 tgtggcctgt ctcgatccac ttgcctgcgc aagagtgcag ggccgcaacg tcatccggct
    17641 cggacgcgaa gatcatctca gcgcgtggcg tgtggtcgac ggtccgcacg tttacgattt
    17701 tgcggcgctg ctcgatggca gcatcgacgc cgatcttgcc cgccgcgatt tcaccgtcaa
    17761 cgccatggcc gtcgatctgc gctcgggcgc attgctcgac ccgcacggcg gccgcgctga
    17821 cctcaaagcg cgcatcgtcc gcatggtcga cgccagcaac ttcgacgacg atccgctgcg
    17881 gatggtcaag gcggtgcgga tggcggtgcg cttcggtttc tcgatcgatg acgccacgct
    17941 gacggcgatt cgcgaacgtg ctccgcgcgt tgccgatgtg gcggtcgaac gggttgcgta
    18001 cgagctgacc gtgatctttt ccgcgaagtc cttccgtcgc gccctagcct tgctgcacga
    18061 gacagcgctc gacgtgccgc ttttcgaccg ggcactcgat ccaaacgcgt tcgaagcgga
    18121 tgacgtgccg ttggccgcgg cgctggcgct gctggttccg gacgccgagg ggtttgcgaa
    18181 acggtggcga ctgagcggga cgctgctgcg ggacgttgtc acgctgcaac atctcagcga
    18241 tgctcactct ctgctcgacc tgtacgacgc cggcgagatc gtcgccgggc agttgacgcc
    18301 gatgctgcgg gcagtcggcc gcgaggagcg catcgacctt cccgacttcg cgatgcggcc
    18361 gctgctcacc ggcgacgaga tcgcctcgct ggccgcaatc gctgccggtc cgcgcgtcgg
    18421 tcagttgaaa cgcgcgctgc tcgaggaaca gctttccggc cggatcacga cgcgcgagga
    18481 cgccgtcgcc ttcatcactg cgtcagcgtg acgtgtacct cgccgaaggt cgagtggacg
    18541 ctcacgtcgg atcgccccgc ggcgttgtaa aagtgcgcag tgcggggaaa gacgccctcg
    18601 ttctcgatgt agtgatcgcc gaagtaggtg tgcacctcgc cgatgagcac cgaggcgcgc
    18661 aggtcgcgga cgttcacgcg gggagtccgg acgtgaactt cgccggcgcg caagtccgtg
    18721 tcgattctgc cgaacgcgcc gtcgatgttg acctcgccgt agcgcgtgga gacgtcgacg
    18781 tcgactcccg gcggcacgtc gaccgtgaca tcgacctcgg tatgaatgct ctttgcgctc
    18841 cagctctgcg cgccggggcc gtagtgccgc cgcacggttg cctcgtcgcc gtcgatcgcg
    18901 atctcggtgc tgatgtcgtc cgcgatcgac tgttccttgg cccggcgccg gtagccgtcg
    18961 aagtggcggc ggacttcgcc gcggacgacg atgcggtctg aggcgccgtt gcggatcttc
    19021 acgctgccgg ccggaatgtc gatgacgacg cggtggacgt gcgcgcgtgc cgaggatgtg
    19081 gagaaggagt gggttacctc gtcatcggcc gctgcggctc cagccaggag cagcgccgcc
    19141 gccacgaaga gaagttgctt gcgcatgggg tgtgtcctcc acgatagaga acggtgcgcg
    19201 agcgaaaagg ttatcgaaaa aaaacgaggg gcccgaaggc ccctcgagta cgactcccca
    19261 aaagtcgatg aagtgcttac ttcttcttcg ctgacttctt agctggcttc ttcgctgctg
    19321 cctttttctt tgcggccatc aagctcacct gcctttcatg ccgtacggga cctcacggtc
    19381 gcgcctactt ggcaaaacac gccggatttc tccgggcaat cgtttttcgg gttggggttg
    19441 cggcggggtg tgaagagacg acgaccttcg acattcaccg gagcttcgag gttgttcttc
    19501 cagttcgtgg cgatcatcgt tttaacttgt cgttaatatg cgatcgttaa tccatattgt
    19561 caagcaattg tttatgaaaa tcgtaatcga gacgcgatgt tccacgtccg ttccacgaaa
    19621 aagagtgctt ttcagcgcgt catcgaggcg cgatacgcgc cgcgcgtgcg ctccgcgcag
    19681 cgcatccacg tgaagttttt cgcgcgctcg atgccgcgcc gcgcgagatc ggcgcgcaac
    19741 gccgcgtcgg atgcgacgcg ctgcatcgca tcggcgagcg catcgatcgc cggctcggca
    19801 tgcagtgccg catcacccgt gatctccacg agcgccgccg cattcgacgt gatcaccgcg
    19861 catccgcagg ccatcgcttc gagtgcggga aggccgaagc cctcttcgcg gctcggcatc
    19921 accagcgcaa tcgcgccgcg gtagagcgcc gccagctcgt catcgctcac gaatccgctg
    19981 acgacgacgc gctcgcggtc gcggaagcgc tcgaacggcg caccggtgag aacgagcgag
    20041 gcgccgtcga ttctgtcgaa tgcgtcgacg agcacatcga cgtttttgtg cggcttgtcg
    20101 ttgccgacgt agaggaagta gcgtccttcc gccgcgttgc cctccgcagc gaaaggcggg
    20161 ccgacaccgt tcggcgtgac gacgacgtgc tcgtcggcgc agccgaacgt agctacgatc
    20221 tcgcgcttca cgctttcgct caccgtcagc acgcgctggc tgcggcgcac cgcgcggccg
    20281 agcatctgcc gggcgtacat ccgcgccagc gggttcggat gacgaaggtg gatgagatcg
    20341 tggaccgtga cgacaagggg cacgctagtg aagggaacga cgtagtgcgg ggcgtggaag
    20401 agatcgatct tcgcgcggtc ggcggcccgt ccgaccgcaa cgagctcgcg gatcgagtag
    20461 tgcggcgcgt cgaggaccac gtgctcgatc cctgccggca gaaaccgggc atggctctcc
    20521 ggtgcgaagg cgacgtaggt gtcgccgtcc cgttgcgcca cgagagcacc cagcaatccg
    20581 cggatgtacg tgccgatccc gaagtcggca atcttcctgg cgtcgatccc gattctcacc
    20641 gcgccgcctc agcaagaagt tgtgtcatca atgccgccga ccgctcccac gagtaagcgc
    20701 gcgcccgctc agcgccgcga ctgcgcagtt catcgcgcaa tgtttgatcg ccgaggagtt
    20761 gaaggagcga ggcgcgaagg gcgttgcggt cgcccggatc gatgagcagc gcggcgccgt
    20821 ccgcatattc gggaatggcc cccgtgcgcg tggcgatgac cggagagcca cacgccatcg
    20881 cctcgagcgg aggaatgccg aagccttcgt agcgcgaggg atagacgaag gcgcgcgcac
    20941 cgcggtagag gtcgcgcagc gtcgtgcgat cgacgtagcc agtgacttcc gcgccgttcg
    21001 ggagctgcac gtcgccccag ccggcgtcgc cgcacaacac gagccgcggg cgcggctgtg
    21061 gcagcgagtc ccaaacagcc agcaggtcgt cgagcccttt gcgcggctcg atcgtgccga
    21121 cgtagaggac gtagtcggat tctcctgcct cgatcgccga cgagaagaag tcgtcgacgc
    21181 cgttcggaac aatctcgatc cgctccctcg ccactccgaa ccaccttacc gcctcctcgg
    21241 ccaccgcacg cgagaccgcg gcaatgcgcg tagccatctg cagactgcgt ccgatgaacg
    21301 cgttgaaaga gagcacggtc ttcgcgcgat gccgaaacgg catggtcagc gagctgaaat
    21361 cgtgcagcgt cgccaccgac gggatgcgca gcgcgagcgg gagcgttccg tgcggtcccc
    21421 acacgacgcc gctcttcgcg atgcgaggaa gtacgaactg ttgccacaag acgccgagcg
    21481 ccatcggatc gacgcgaaag cggcagcgct cgagtcccga ccgatcggcg atctccgcat
    21541 gcgacgcgat gagcggggca tcgggtagac gccgcgcgat ctcggcagtg tgaacgccga
    21601 tccctgtgcg gttgccgacg agggcgcgtc cgtcgatcgt gacttggaag ttcaatggat
    21661 caagtgtagc ttcctgtgag gctcaccaca gagactcaga ggacacagag ataagcgaaa
    21721 cctctgtgcc ctctctgtct ctgtggtgct tcaagaaccc gccgccttcc gcaaaaccgc
    21781 cagcgtctgt tcggcggcca catcccaacg gaactgcgcc gcgcgcagcg gtcctgccgc
    21841 agcgaggtgc tcgcgaagag cagcatccat cagcacgcgg gagatctgcg cttcgaggcc
    21901 tttagcatca tgcggttcga agtagagcgc ggcgtcgccg ccgatctccg gcagcgacga
    21961 cgaacgtgct gcgatcgacg gaacgccgta cgccatcgct tcg
//
LOCUS       DATLVW010000086         6392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_170057, whole genome shotgun sequence.
ACCESSION   DATLVW010000086 DATLVW010000000
VERSION     DATLVW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170057"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VNN25_02905"
     CDS             complement(<1..396)
                     /locus_tag="VNN25_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HXH90504.1"
                     /translation="MTLRWLVTGAGGMVGHDVRAALETRGEEVVALSKSELDITDGTV
                     VSEAVRDARPDVIVNCAAYTKVDDAEKKEHLATAINGSAVEFLAHAADRAGALLVQLS
                     TDFVFDGSKRTPYEINDPTAPLSAYGRSKL"
     gene            complement(419..610)
                     /locus_tag="VNN25_02910"
     CDS             complement(419..610)
                     /locus_tag="VNN25_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXH90505.1"
                     /translation="MFGTLGLPELLVIFAIILVVFGAGKLPQLGKGLGEGISNFRDGL
                     KGRDDKKAATPSLEEKKNA"
     gene            763..3129
                     /locus_tag="VNN25_02915"
     CDS             763..3129
                     /locus_tag="VNN25_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXH90506.1"
                     /translation="MIDLTGLNPQQREAVTHGEGPLLVLAGAGSGKTRVITYRIAWLI
                     GELGVHPTNILAVTFTNKAASEMVSRVGLLAPNSGARAHGSLWIGTFHSTSLRMLRRH
                     AERLGYTKSFVVYDTSDQLTLVRRCMRELQMNDEAFPPRSILNRISGAKNELIGPAEY
                     EKETLDFFGSRVAEVYRMYQRRLKEYDAMDFDDLIGNYVQLIEKNEDVAEEIHNRFQH
                     LLIDEYQDTNRAQYMLIKALAGKKQNIVAVGDEDQSIYKFRGADINNILNFERDFPGA
                     RIIKLEQNYRSTGNILGAATGVVSNNIARKGKTLFTDSGSGEPVRVVSCTNEREEAQF
                     VIEKITNGRGKYKLSDYAILFRTNAQSRPFEEELLRSNIPYSVVGGVKFYERAEIKDI
                     LSFLRLTIRPHDTPSIERVINVPSRGIGDTTTKALNDQAAAQNVTLWTIIEGELAFLP
                     PRASKAVKEFREIVHDLNRAANNPLPELYDYLLLRTGYRRMLQESRDLQDESRLQNID
                     EMMNSAREYSEQNPGASLGDYLDSLTLMSDLDKYESQKGVTLMTLHAAKGLEFKVVFL
                     TGMEEGILPHSQSKDSNDDLEEERRLCYVGMTRAREQLWCVHCLERRLHGQFREQSPS
                     PFITEIPEEVREEVRMARARYVPEAQSWRERPMGSYDRYGDRYSNEGRRAPSYGRPTA
                     PARPAPPPRPVAVAPPQPRKNDSVNGVLSFFKESPVQLDPAALRPATQQSAPSADFKR
                     AQRVRHEQFGDGVILTMEGSGPDAKLTVYFDRIGSKKFIAKYAKLIRI"
     gene            3156..4343
                     /locus_tag="VNN25_02920"
     CDS             3156..4343
                     /locus_tag="VNN25_02920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXH90507.1"
                     /translation="MNGGSTLPLEGILVADFSRVLAGPLATQLLADSGARVIKVEEPG
                     GGDETRRWGPPFVGGISAYFLSVNRGKESVTLNLRSEDGWEAARRLIERADVVIDNFL
                     PAQKASLRFAPRHINPRAVHCTIGGFDSDSAEANTPGYDLLAQAGSGLMSITGEEDGP
                     PMKIGVALADVLTAHYAFGAICAALRGRERTGEGCSLEVSLFGATIASLVNVAQNVLV
                     TNREAGRYGNAHPSIVPYQVFHGSDRAFAIGAGTDRHFTQLCERVLRRPELAREARFA
                     TNAQRVKHRKTLVPLLEEEFSKRTAAQWVARCRRAGIPASLVRGVREALRTPRARPLV
                     ETVEHPEIGSYEAIRNPVRIDGRRRAIGSAPPTLGQHTNAILRELGFDQRSISAMRKS
                     GAI"
     gene            complement(5027..5254)
                     /locus_tag="VNN25_02925"
     CDS             complement(5027..5254)
                     /locus_tag="VNN25_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90508.1"
                     /translation="MNGIDPEFQQILVRVVMVGGTIMIVGATGLFFAFRAFAPSAKKG
                     SDFRAVVAIIAVLAVVMIGCFILLIFSYAKR"
     gene            complement(5251..5856)
                     /locus_tag="VNN25_02930"
     CDS             complement(5251..5856)
                     /locus_tag="VNN25_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90509.1"
                     /translation="MARPILGTFNQQNALVLDLGISGAFIEHYGVATSGLNCRLQFPW
                     KGEPIEFAGRVARTFVVRVSGNEVLSHTGIRFTEPVGESESRLHDLMAVFVGSILAAQ
                     KANKDGVRLDEDHLTLMDVGGARRTRVRGLKRYRMEPDGTWNHGTTTDPNQLADGFTV
                     AAYEYEEDLEMLCRAYELADEEGRRLIRLVAELSVRTVVAS"
     gene            5954..>6392
                     /locus_tag="VNN25_02935"
     CDS             5954..>6392
                     /locus_tag="VNN25_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90510.1"
                     /translation="MTERQTERRSIWRVGPDSTWTTVIPVVFIIASLLTLVFLPVLIG
                     NRTAQMRAEITDVAEPARKAANKIETDLSTELNEIIAYQVTTQPQYVRNYQDLLTNQQ
                     SSDRLLHRLGPRLGPAVQGHLASLEKESREWHADVASNEFLKRQ"
BASE COUNT         1306 a         1970 c         1945 g         1171 t
ORIGIN      
        1 gagcttcgag cgcccgtacg cggaaagggg ggcagtcgga tcattgatct cgtagggagt
       61 ccgcttcgag ccgtcgaaaa caaaatcggt cgagagctgg acgagcagcg cacctgcgcg
      121 gtcggcggca tgcgcaagaa actcgacggc cgaaccgttg atcgcggtcg ccagatgctc
      181 cttcttttcc gcgtcgtcga ccttcgtgta cgcggcgcag ttgacgatca cgtcgggccg
      241 cgcatcgcgg actgcttcgc taacgacggt gccgtcggtg atgtcgagct cgctcttcga
      301 caaggcaacg acttcttcgc cgcgcgtttc cagcgccgcg cgaacgtcgt ggccgaccat
      361 cccgcccgct ccggtgacga gccagcgaag cgtcacgggc acctcagcgc gcagagtttc
      421 aggcgttctt cttttcttcg agcgacggtg ttgcagcctt cttgtcgtcc cgtcccttca
      481 gcccgtcgcg gaagttggag atgccttcgc cgaggccctt gccgagttgc ggcagcttgc
      541 cggcgccaaa gacgacgagg atgatggcga agatgacgag aagctcaggt aatcccagtg
      601 tgccgaacat gttagatagc ctccgaagtg gttgccgcgg cggcccctga ctccccgcgt
      661 tggataccgc aaagtgtagt acgaattctc ttcccaatca ggtaacagcg ctcacggggt
      721 cttggatttc gcgtcacagc gcggctgtta ccatgcaacc cagtgatcga tcttaccgga
      781 ctcaatccac agcagcgcga agccgtgacc catggcgaag gaccgcttct cgtcctggct
      841 ggcgcggggt cgggcaaaac gcgtgtcatt acctatcgga tcgcctggct gatcggcgag
      901 ctcggtgtgc atccgacgaa catcctcgcg gtaacgttta ccaacaaggc tgccagcgag
      961 atggtgagcc gcgttggcct tctcgctccc aacagcggcg cgcgcgctca cggcagcctt
     1021 tggatcggca cgttccactc cacgtccctt cgcatgttgc gccgccacgc cgagcgcctc
     1081 ggatacacca agtccttcgt cgtctatgac acctccgacc agctcacgct cgtgcgccgc
     1141 tgcatgcgcg agctgcagat gaacgacgag gcgtttcctc ctcgttcgat tctgaaccgc
     1201 atctcaggcg ccaagaacga gctgatcggg ccggccgagt acgaaaaaga gacgctcgac
     1261 ttcttcggat cgagggtggc cgaggtctat cggatgtacc agcggcgcct gaaagagtac
     1321 gacgctatgg atttcgacga tctcatcggc aactacgtcc agctcatcga gaagaacgag
     1381 gacgtcgccg aagagatcca caaccgcttc cagcaccttc tgatcgatga gtaccaggac
     1441 accaaccgcg cgcagtacat gctgatcaag gcgctggccg gcaagaagca gaacatcgta
     1501 gcggtcggcg atgaagacca gtcgatctac aagttccgcg gcgccgacat caacaacatc
     1561 ctcaatttcg agcgcgactt tccgggcgcg cgaatcatca agctcgaaca gaattaccgt
     1621 tcgaccggca acatcctcgg cgcggccacc ggcgtggtgt cgaacaacat cgcccgcaag
     1681 gggaagacgc tcttcaccga ctccggcagc ggcgagccgg tgcgcgtggt gtcgtgcacc
     1741 aacgagcgcg aagaggcgca gttcgtgatc gagaagatca ccaacgggcg tgggaagtac
     1801 aaactctcag attacgcgat tctgtttcga acgaacgcgc agtcccggcc gttcgaagaa
     1861 gagttgctcc gttcgaacat cccctactcc gtcgtgggcg gcgtgaagtt ctacgagcgg
     1921 gccgagatca aggacattct ctcgtttctc cgcctgacga tccggccgca cgacacgccg
     1981 tcgatcgagc gcgtgatcaa cgtgccgtcg cgcggcatcg gcgatacgac gacgaaagcg
     2041 ttgaacgatc aggccgcggc gcagaacgtc acgctgtgga cgatcatcga aggcgaactt
     2101 gcgttcctgc cgccgcgcgc ttcgaaggca gtcaaagaat ttcgcgagat cgtgcacgac
     2161 ctgaatcgcg cggcgaacaa tccgctgccc gagttgtacg actatctgct cctgcgcacg
     2221 ggctatcggc ggatgctgca ggagtcgcgc gatctccagg acgagtcacg cctgcagaac
     2281 atcgacgaaa tgatgaactc tgcgcgggag tactcggagc agaaccccgg cgcgtcgctc
     2341 ggcgactacc tcgattcgct gacgctgatg tccgatctcg acaaatacga atcgcagaag
     2401 ggcgtgacgc tgatgacgct gcacgccgcg aaagggctcg agttcaaggt cgtgttcctt
     2461 acgggcatgg aagagggaat cctgccgcac tcgcagtcga aggattcgaa tgacgatctc
     2521 gaagaggagc gccgcctctg ctacgtcggg atgacccgcg cccgcgaaca actctggtgc
     2581 gtacactgcc tggagcgccg ccttcacggg cagtttcgcg aacagagccc gtcgccgttc
     2641 atcacggaga tcccggaaga ggtacgcgag gaagtgcgca tggcccgggc gcgctatgtg
     2701 ccggaagcgc aatcgtggcg cgagcgtccg atggggtcgt acgatcggta cggcgaccgc
     2761 tactcgaacg aaggccggcg cgcgccctcc tacggacggc ccacagcgcc cgcccgcccg
     2821 gctcccccgc cgcgtcccgt tgcagttgcg ccaccacaac cgcgcaagaa cgactcggtc
     2881 aacggagtgc tgtccttctt caaagaatcg cccgtgcaac tcgatccggc ggcgctccgt
     2941 cctgccacgc agcagagtgc gccgagcgcc gacttcaaac gggcgcagcg cgtacgccat
     3001 gaacagttcg gcgacggcgt cattctgacg atggaaggct ccggccccga cgccaagctc
     3061 accgtctatt tcgaccgcat cggctcgaag aagttcatcg ccaagtatgc gaagctgatt
     3121 cggatctgag cacgcgaaga cgcgaaggcg cgaagatgaa tggtggatcg acgctccccc
     3181 tcgaaggcat cctcgtcgcc gatttctcgc gtgtcctggc cggcccgctg gcgacccaac
     3241 ttcttgccga ctcgggagcc cgcgtcatca aagtcgagga acctggaggc ggcgacgaga
     3301 cgcgacgctg ggggcctccg ttcgttggcg gcatcagcgc gtactttctg tcggtcaatc
     3361 ggggcaagga atcggtcacg ctgaatctga ggtccgagga tggatgggag gcggcgcggc
     3421 ggctcatcga gcgggccgat gtcgtgatcg acaacttcct gcccgcacag aaagcatcgc
     3481 tgcgatttgc tcctcggcac atcaatccgc gcgcggttca ttgcacgatc ggcggattcg
     3541 acagcgacag tgccgaggcg aatacgcccg gttatgacct gctggcccag gcaggctccg
     3601 gtttgatgtc gatcaccggc gaggaggacg gaccgccgat gaagattggc gtcgcgctgg
     3661 ccgacgttct gaccgcgcac tacgcgttcg gggcgatctg tgctgcgctt cgcgggcgcg
     3721 agcgcacggg cgaagggtgc tcgctggaag tctcgctatt cggcgcgacg atcgcctcgc
     3781 tcgtgaatgt cgcgcagaac gtgctcgtca cgaaccgcga agctgggcgc tacggaaacg
     3841 cgcatccgtc gatcgttccg tatcaggtct ttcatggcag cgaccgcgcg ttcgcgatcg
     3901 gcgccggcac cgaccgtcat ttcacgcagc tctgcgaacg cgtgctccgc cggccggagc
     3961 tggcgcgcga ggcgcgtttc gcgaccaacg cccagcgggt gaagcatcgc aagacgctcg
     4021 tgccgctgct cgaggaagaa ttcagcaaac gcacggcggc tcagtgggtt gcacggtgcc
     4081 ggcgcgcagg catccctgcc tcgctcgttc gcggcgtgcg agaggcgctc cgaacgccga
     4141 gggcacggcc gcttgtggag acggtcgagc atcccgaaat tgggtcgtac gaagccatcc
     4201 gcaacccggt acgcatcgac gggcgtcggc gcgccattgg ctccgcgccg ccaacgctcg
     4261 ggcagcatac gaatgccatc cttcgcgagc ttggcttcga ccagcgctcg atcagtgcaa
     4321 tgcgaaagag cggcgcgatc tgagatcaac aaatctaccg gccgcgcgga gaaggtagga
     4381 aggggtcatc ctcggggtgc cgttgacgaa gactcgaata tgtccatgcg cttattgagt
     4441 gctgcaaaac ggcgagcaca cgggcgcgaa agtggggagg gtggtggttc ggatagcggg
     4501 ggcagtaggt tttatctgcg tctatctgcg ctcatctgcg ggtcgactgc tttttgatgg
     4561 ggtccaggac accaatcaaa tgtccatgga tacttatagg tcagcgatca gcagctcgaa
     4621 tctatccatg gagtagataa aactaccatt ccggctggcg aaattaggcc atggcaaatt
     4681 taaggtctgg aagccagatc cataaaatgt ccatggacta atttagcgag tgccatggac
     4741 actttcggta atggttttgc ctaccgtatt tgtccatggc actatttcac gagtcgaaga
     4801 gccacgctaa atatggctat gggtactttc ggtagtcgat ttcataattt cggtagatcc
     4861 aaggatgaat tcgacgagtg tcgaagcctc ttcgcgaagg attttggcta ccatatatgt
     4921 ccatggacat catttgcgac ctcggaggct tcgcagcgcg cgggcaggcc tggatactcg
     4981 accgccagag ctagaaacta actggggttc agatctccct tcagtcttaa cgtttcgcgt
     5041 acgagaagat cagcaggatg aagcagccga tcatcacgac agccagcacg gcaatgatcg
     5101 cgacgacggc ccgaaaatca ctccccttct tcgcagacgg cgcgaacgcg cggaaagcga
     5161 agaagaggcc cgtcgcaccg acgatcatga tcgtgccgcc gaccatgacg acacgcacca
     5221 ggatctgctg aaactcggga tcgattccgt tcacgaagcc acaaccgttc gcacgctaag
     5281 ctccgcgacc agacggatca gtcgccgccc ttcctcgtcg gcaagctcgt acgcgcggca
     5341 cagcatctcg agatcttctt catactcata agcggccacc gtgaaaccat cagcgagctg
     5401 gttcggatcg gtcgtcgttc catgattcca ggttccgtcc ggctccatcc ggtagcgttt
     5461 cagccctcgc acgcgcgtgc ggcgcgcgcc accgacgtcc atcagtgtca ggtgatcttc
     5521 gtcgaggcgt acgccgtcct tgttcgcttt ctgcgcagcc aggatcgatc cgacgaagac
     5581 cgccatcagg tcgtgcagac gcgattcgga ttccccaaca ggctcggtga accgaatgcc
     5641 ggtgtgcgat aggacttcat tgcccgagac gcggacgacg aacgtgcgtg ccaccctccc
     5701 ggcaaattcg atcggctcac ctttccaggg aaactgtaac ctgcagttca acccactcgt
     5761 cgcaacaccg tagtgctcga tgaaggcgcc gctgatgccg aggtcgagga cgagcgcatt
     5821 ctgttgattg aacgtgccga ggattggtcg ggccaatcga aggcgttgga agactctgcg
     5881 atcgtttacg tgcatggggc gtggcgaagg caagcatatt gctgcatttt ggcgacagcg
     5941 tccaaggagg accgtgacag agcggcaaac ggaacggcga agcatctggc gagtaggtcc
     6001 ggacagcacg tggacgaccg tgatcccggt cgtcttcatc atcgcgtcgc tgctcactct
     6061 cgtcttcctg ccggtgctca tcggaaaccg gacggcgcag atgcgcgcgg agatcacaga
     6121 tgtcgccgaa ccggcgcgca aagcagcgaa caagatcgag acagatctct cgacggagct
     6181 caacgagatc atcgcctatc aggtaaccac ccagcctcag tacgtgcgca actaccagga
     6241 tcttcttacc aatcagcaga gcagcgaccg tcttctgcac cggttgggac cacgcctcgg
     6301 tcccgccgtg cagggccacc ttgcctcact cgagaaagaa agccgcgagt ggcatgccga
     6361 cgtcgcgtcg aacgagtttc tgaaacggca ga
//
LOCUS       DATLVW010000087         4366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_3114, whole genome shotgun sequence.
ACCESSION   DATLVW010000087 DATLVW010000000
VERSION     DATLVW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3114"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1792
                     /locus_tag="VNN25_02940"
     CDS             242..1792
                     /locus_tag="VNN25_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90511.1"
                     /translation="MIRWLALFVLAAVSAQAAEPLRVGRITIDAVPVFSGAEEAHGRF
                     YGLMNVLHVQTRVALLRRFLLFREGDPYDPALIEETERALRLFDFLESASVTASAPHD
                     GVVDVAVVTHDALTTAVNADFSNEGGIAAYSADVTQKDLFGSGSSLDLHTEHGIERSG
                     TAVELTDPALFGPYWNLDTLYAKNSDGSEEKVAIDRPLYSYRTPWTASALFDQLQRNE
                     RIFALGEVAARFRHEHRELALSRSKVLHADREGSSSIVAAIDLLDDSFGALPARPDDV
                     IPRARHFRFVEAGYERIGFRFVKLDYVDSDIREQDFNLGLYATIRAAISPSQSSDRPL
                     TFRLRAAAGSGYAFSERAFVIGQITASTRAPRDRNTTIAIEGRSVSRFQTRYPQAFVT
                     RAHLDLGWQLDHDVQFLADGQNGLRAYPDFAFEGSRRLIVNAEHRISLGRELLQFVGP
                     GVAVFADSGQAVDGPFRGMKSDAGAGLRLGVSRLDALIRIDWAYAFNASPLSRRGPVW
                     SISTSQAF"
     gene            complement(1876..2685)
                     /locus_tag="VNN25_02945"
     CDS             complement(1876..2685)
                     /locus_tag="VNN25_02945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH90512.1"
                     /translation="MIRNATRDDLPRIVKLLARANDAPYDLVRVAEEKCFGAGVGGEP
                     EVRVADDFAGISVTCGTYLRILAVDRARRGRGVGSALLREARERGRAQVVAAEAGNYF
                     TPGIAATDEPAIDFFVKRGYREIASTQNLVASVESGKPGVGVVRATPASRERILHFIE
                     KEFGRIWRFEAGRAFENDPPTLFYVEVDGEIAGFAAHDANNRGLGFFGPTGVARAHRG
                     RGLGARLLHASLADLHRLGYQSAVIPWTDAIEFYRKACGATVAERFVTLGV"
     gene            2728..3111
                     /locus_tag="VNN25_02950"
     CDS             2728..3111
                     /locus_tag="VNN25_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90513.1"
                     /translation="MSPVVLWSKRPEEKVLRHETTSLANIRLSRPSVGAERATIVQEA
                     SVKTKRELLQDLVGTTINVTAEHVILPVPKHTPGLHASTMATLTEMGEDMIHVVYEHY
                     HRNPGDILEQWITIDSITEIKRCRT"
     gene            3144..3461
                     /locus_tag="VNN25_02955"
     CDS             3144..3461
                     /locus_tag="VNN25_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90514.1"
                     /translation="MKNLDTRILLTFMLVVMFFAGGMLLNYFASREQVTLGEKEQASD
                     VKLIEYVKSKADNFSQSAKVLFDFGKIALGALLTMLSGVKAPTTAPTIAPQDAVPRRA
                     FDE"
     gene            3577..4008
                     /gene="trxA"
                     /locus_tag="VNN25_02960"
     CDS             3577..4008
                     /gene="trxA"
                     /locus_tag="VNN25_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXH90515.1"
                     /translation="MDEIVRCASCGQANRVPPVGSGRKAVCGRCKAPLTAGSDGHPVD
                     VTDANLAETIRHGAYVVDFWAAWCGPCRMIAPIIEELAASRSDVHFAKLNVDQNPQAA
                     SQYRAMSIPLLVFFKDGVEAGRVVGAVPRGRIEAAIKQYFG"
     gene            4162..>4366
                     /locus_tag="VNN25_02965"
     CDS             4162..>4366
                     /locus_tag="VNN25_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH90516.1"
                     /translation="MVKLPTVDRVISLVRKPGQPLPSAAASKTRESRLRGAFDHAPVG
                     IALASMHGTWLETNERFCELIGYT"
BASE COUNT          801 a         1400 c         1329 g          836 t
ORIGIN      
        1 cgttctggta ccgcccgagc ctctacctcg gcttcggctt cggcgggttc tacggcggcg
       61 gattccgcgg aggccacttc ggtggtcacg gcggtcgtca tcactaactc gtagtaagcc
      121 tcgttcacgg tcgacgaaag cccgggcctc gcgcccgggt tttttttgtg gcggtgatga
      181 ggatttgcgg cctgtcccca gcttcaccgt cgcgtcatgt ttgggctgat acttagcgcc
      241 cgtgatccga tggcttgcgc ttttcgttct cgccgcggtg agcgcgcagg ccgcggagcc
      301 gttgcgcgtc gggcgcatca ccatcgacgc cgtgcctgtc ttcagtggcg ccgaggaggc
      361 gcatgggcgg ttttatgggc tcatgaacgt gcttcacgtg cagacgcgag ttgccctgct
      421 gcgccggttt ctgctcttcc gcgaagggga tccgtacgat cctgcactaa ttgaagagac
      481 cgagcgcgca ctgcggctgt tcgactttct ggagagcgcg tcggtcaccg catcggcgcc
      541 gcatgacggc gtcgtcgacg ttgcggtcgt cactcacgat gcgctgacga cggccgtcaa
      601 cgcggacttc tcgaatgagg gagggatcgc cgcgtattcg gccgacgtca cgcagaagga
      661 tctcttcgga tcaggcagtt cgctcgatct gcatacggag cacggcatcg aacggagcgg
      721 gacagcggtc gagcttaccg atccggcgct cttcggaccg tattggaacc tcgacacgct
      781 ctacgcgaag aactcggatg gcagcgaaga gaaggtggcg atcgaccggc cgctctactc
      841 ctaccggacg ccctggaccg cgagtgcgct cttcgatcaa ctgcagcgca acgaacggat
      901 cttcgcgctc ggggaagtgg cggcgcggtt ccggcacgag catcgcgagc ttgcgctgtc
      961 ccgctcgaag gtcctgcacg ccgatcggga agggtcttcg agcatcgtcg ctgcgatcga
     1021 cctgctcgat gattcgttcg gggcgttgcc ggcgcgtccg gacgatgtca ttccgcgtgc
     1081 gcgtcacttc cgcttcgtcg aagccggcta cgagagaatc ggattccgct tcgtcaagct
     1141 cgattacgtc gacagtgaca ttcgcgagca ggatttcaac ctcgggctct acgcgacgat
     1201 tcgcgcggcg atctcgcctt cgcaatcgag cgatcgtccc ttgaccttcc ggcttcgcgc
     1261 tgccgccgga tcgggctacg cattcagcga gcgtgcgttc gtcatcggcc agatcaccgc
     1321 gtccacgcgc gcgccccgcg accgcaacac gacgatcgcg atcgaaggcc gctccgtatc
     1381 gcgctttcaa acgcgctacc cacaggcatt cgtcacgcga gctcacctcg atctgggctg
     1441 gcagctcgat cacgacgtgc agtttcttgc cgacggtcag aacggacttc gcgcctatcc
     1501 cgacttcgct ttcgaaggaa gcaggcggct gatcgtgaat gcggagcatc gcatttctct
     1561 cggacgcgag ctgctccagt tcgtcggccc cggtgtggcg gtgttcgccg attccggaca
     1621 agcggttgat ggtccattcc gaggaatgaa gagcgatgcc ggagccggat tgcgcctcgg
     1681 cgtctcccgc ctcgacgcgc tgatccggat cgattgggcc tacgcgttca atgccagtcc
     1741 actcagccgc cgcggtcctg tctggtcgat ctcgacatcg caggcgttct gaacgtagtg
     1801 caggcattct tgcctgcaac cgccgggcgt ccctgcccgg cggtcattgg atgccagatt
     1861 gttcgctcgc gatactcaca ctccgagcgt cacaaaccgc tccgctaccg tcgccccgca
     1921 cgctttgcga taaaactcga tcgcgtcggt ccagggaatc acggcgctct ggtaaccgag
     1981 gcggtgcagg tcggcgagcg aggcgtgaag cagtcgcgcg ccgaggccgc ggccgcggtg
     2041 ggcgcgggca acgcccgttg ggccgaaaaa tccgagtccg cgattgttcg catcgtgcgc
     2101 ggcgaagccg gcaatctcgc cgtccacttc gacatagaaa agagtgggcg gatcgttctc
     2161 gaacgcacgt ccggcctcga atcgccagat gcgcccgaat tctttttcga tgaaatggag
     2221 aatgcgttcg cgtgaggccg gcgtcgcgcg aactactccc acccccggct tcccactttc
     2281 gacgctcgcc accagattct gcgtcgacgc gatctcgcgg tatccgcgct tgacgaagaa
     2341 atcgatcgcc ggctcatcgg tggccgcgat gccgggcgtg aagtagttgc ccgcctcggc
     2401 agcgacgact tgcgcgcgtc cgcgctcccg cgcctcacgc agcagcgcgc tccccacgcc
     2461 gcgtccccgg cgcgcgcgat cgaccgccag gatgcgaagg tacgtcccgc aggtgacgct
     2521 gatcccggca aagtcatcag cgacgcgcac ctcgggttca ccgccaacac cggcgccaaa
     2581 gcacttctcc tcggcgacgc ggacaagatc gtagggcgcg tcgttcgccc gcgccaacaa
     2641 cttcacgatg cgcggcaagt cgtcgcgcgt ggcgtttcgg atcacgagtg ggattacacc
     2701 acggtagggg agtgggggaa tcgcgcaatg tcaccggtgg tgctgtggag caaaaggccg
     2761 gaagaaaagg tgcttcgaca cgaaacgaca agtctcgcca atattcgcct gagccgaccg
     2821 agtgtcggcg ccgaacgcgc tacgattgtc caggaggctt ccgtgaagac aaagcgagaa
     2881 ctcttacagg accttgtcgg cacaacgatc aacgtaactg cggaacacgt gatccttccg
     2941 gtaccgaagc acacgcccgg cctgcatgcg tccacgatgg cgacgctcac agaaatgggc
     3001 gaggatatga tccatgtcgt gtacgagcac taccaccgca atcctggcga tatcctggag
     3061 caatggatca ccatcgattc gataaccgag atcaaacgtt gtcgaacttg accaatgcct
     3121 atctttgaga aggagccgct aagatgaaga atctcgatac aagaatcctt ctgacgttca
     3181 tgctagtcgt aatgttcttt gccggtggca tgctgctcaa ttacttcgct tcacgcgagc
     3241 aggtcacact cggcgaaaag gaacaggcct cggatgtaaa gctcatcgaa tacgtaaaga
     3301 gcaaagccga caacttctcg cagtcagcca aggttctctt tgacttcggc aagatcgcgc
     3361 tcggcgccct actgactatg ctctcaggtg taaaggcgcc gactaccgcc ccaaccatcg
     3421 caccccaaga cgccgtgcca cgccgcgcct ttgatgaatg actcccgtga ggtattcctc
     3481 ggcgttccgt tcgctcgtga gccaagaaac tcagcaaccc gacacccgac acccgactcc
     3541 caacacccgg cccacgtttc gcaatgcatc ctcgtcatgg acgagatcgt tcgttgtgca
     3601 agttgtggtc aggccaaccg ggttcctccc gtcgggagtg gacggaaggc tgtttgcggt
     3661 cggtgcaagg cgcctctgac agccggatcg gacggtcatc ccgtcgacgt cacggacgcc
     3721 aatctcgccg agacgattcg tcacggcgcg tacgtcgtcg acttctgggc ggcctggtgc
     3781 gggccgtgcc ggatgatcgc gccgatcatc gaggagctcg ccgcgtcgcg aagtgatgtt
     3841 cacttcgcca agctcaatgt cgatcagaat cctcaggcgg cgtcgcagta ccgggcgatg
     3901 agcattccgc tgctggtctt tttcaaggat ggcgtggaag ccggccgcgt cgtcggcgcc
     3961 gtgccgcgtg ggcgaatcga ggcggcgatc aagcagtact ttgggtaggt gcttccagga
     4021 aaaccggtca cgcgaagacg cgaagacgcg aagggaagcg cggacagccg gcggttcatg
     4081 cggatctttc tccgaccccc gactcccgac aaccgactcc cttcacaaag tgctaccctt
     4141 tcccgactcc cttaattcaa catggtcaag ctcccgacgg tcgatcgagt catctcgcta
     4201 gtgcgcaaac ctgggcaacc gctgccttcg gccgcggcct cgaagacgcg ggagtcgcgt
     4261 ctgcgcggcg cgttcgacca tgcgccggtc ggaatcgcac tcgcctcgat gcacggcacg
     4321 tggctggaaa ccaacgagcg attctgcgag ctgatcggct acacgc
//
LOCUS       DATLVW010000088         7019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_85841, whole genome shotgun sequence.
ACCESSION   DATLVW010000088 DATLVW010000000
VERSION     DATLVW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85841"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VNN25_02970"
     CDS             complement(<1..396)
                     /locus_tag="VNN25_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXH90517.1"
                     /translation="MKSREQATGKPASPLLDKRIHDPGDWREETLDRMRALILEADPE
                     MIEERKWKKPSNAAGVPVWSHNGIVCTGEMYTKAVKLTFAQGASLPDPSHLFNSSLEG
                     NMRRAIDIHEGEKVDARAFKALVKAAVAQN"
     gene            complement(393..794)
                     /locus_tag="VNN25_02975"
     CDS             complement(393..794)
                     /locus_tag="VNN25_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH90518.1"
                     /translation="MRIKWTTLYVDDQEKALQFYTETLGFQKKADFTNGNYRWLTIAS
                     PEDPDGMELVLESNANPAGKAYQEALRNQSRPAAQFLVKDVQAEHDRLASKGVTFTVP
                     VTRTTGSIIAVLDDTLGNLIQLVQLTWGAGA"
     gene            complement(936..1718)
                     /locus_tag="VNN25_02980"
     CDS             complement(936..1718)
                     /locus_tag="VNN25_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXH90519.1"
                     /translation="MSRDSRFEVPAQPRDRQAEAIISLEKGIRSRDEVLAIVAHDLRN
                     PLTIIKSSAALLQLTTDEVQRKELIEMISTSCGRMQSLVGDLLDVARIESGGFTVDRT
                     DVDAADVIEEMRRMFEPAAAEKGIRFEADASGVLPPLYADRNRLIQILANLVSNALRV
                     TDRGSVRLTVDGDRDKARFSVTDTGPGIPAAELPHLFDRFWQVRSSRRGGAGLGLAIV
                     KGFVEAHQGTIEVTSEVGKGSQFRFVIPSFKDKCSHSMRLPV"
     gene            2004..2834
                     /locus_tag="VNN25_02985"
     CDS             2004..2834
                     /locus_tag="VNN25_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90520.1"
                     /translation="MESRGGRRFALQKAAFGVLAILVVLLSVDVLGVFGAGDRKESGK
                     RIPDATVAAWNKLDADAKQLLDKEHDNVVAEIGHRIEHEHLLFTLKFALVGGILYGLL
                     QGAFGRGDTHIEKTAFAALIVWAAVIAAAIVDLRIMANQSFLVTLGGWSRQYEELRVG
                     ANGVDLAWEAFLANNLLSQPHYPALRVSGQILTALLFAFSGSLFLLSKESDHDPAIAL
                     VSGSCAVLSIGLMTVAAISLRQRGVAVLLYLSFGTVAAILALVLAHTSKRNYRAEIRD
                     "
     gene            complement(2922..4472)
                     /locus_tag="VNN25_02990"
     CDS             complement(2922..4472)
                     /locus_tag="VNN25_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH90521.1"
                     /translation="MAERKVISSRARTWTLAAAILGSCMAFIDGTVVNIAIPVLQRSM
                     GATVSDTQWIVDAYLLVLSSLMLAGGGLGDHLGRRKVFAIGIAMFAVASAVCGAAVNP
                     HQLIAARAFQGLGAALLVPGSLAIITATFPASERGRAIGTWSSLTSLAVIAGPLFGGW
                     LVQAVSWRAVFFINLPIAAITLLIVWRAMPPDQKTKNAGRIDWGGTLLVTFALAALTF
                     GMIEAPSRGMRSALVIGPLLFGTIAFIAFIVLERRIDDPIMPLTLFRSRAFSGANLLT
                     LLLYAALSTVTFLLPFNLIQVQHYSPAQAGGAFLPFVATMSILSRWTGGLADRFGPRL
                     PLIVGPLLACCGFLLLARPGIGGSYWTTFFPAVLVLGLGMATTVAPLTTTVMTSFGEE
                     KHAGAASGINNTVARAAGLLSIALFGALSIMIFARSLDARLAANGASPALRQTMAEQR
                     LRLAEAKPPATLDATEKARVSAAIDDAFIRAFRISMLAAAALAAASAAGALVIGGTIG
                     SSARALGK"
     gene            complement(4454..5428)
                     /locus_tag="VNN25_02995"
     CDS             complement(4454..5428)
                     /locus_tag="VNN25_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXH90522.1"
                     /translation="MASLLPLPTPHPPLPTPSVAFERRRPNAPTSDPRLPTPPTRARV
                     LLSIAILLLAACAKQKPIVVGSKNFTESVLLGEIVAQRLERAGCSVERKLDLGGTLVC
                     DKAITAGALDVYPEYSGTALTAILHHDAMRDRAAVMKKVAADYGARGLSWGPALGFEN
                     TFAMIVRSSDAQRLSLKTISDLGKVSATFRPGFGYEFVERPDGWNGLLQHYGLRFAEA
                     PRTMDLGLTYRALAAGEIDLIAGNSTDGLIDALGLTVLADDRHFFPPYDAALVVRRDL
                     GTKCAAGPAALASLAGSLDATAMRRLNLAIDRDKRSPSEVARTWLKEK"
     gene            complement(5579..6202)
                     /locus_tag="VNN25_03000"
     CDS             complement(5579..6202)
                     /locus_tag="VNN25_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH90523.1"
                     /translation="MSDLTAEILHATAQHLLIVGVAMLVATSAGIPIGIACIRNPRSG
                     RIVLRLIDIIQTVPSLALFGFLIPLPFIGGIGIRTAIVALILYSLLPVVRNTVTGIAG
                     VDPLIREAATALGMTERQRLMRIDLPLALPSIFGGIRIATVIAIGVATIAAAVGGGGL
                     GTLIFRGVAMVDSTRILAGALPAAALAVAADLLFGALERLAARRLGV"
     gene            complement(6199..6945)
                     /locus_tag="VNN25_03005"
     CDS             complement(6199..6945)
                     /locus_tag="VNN25_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH90524.1"
                     /translation="MGSSVVIDRATVAGSNGNAIVRDVSLRIDPGESVAVIGRSGAGK
                     TTLLRLLNGLAAPSTGAVVIDGLPLGEPGLAERRRRIGTILQAPALFPHRTVLENVAT
                     VPRLLGWPDERIRQASAALLQRLDLPFDRFAARFPRSLSGGEQQRVGIARAMVADPPL
                     LLCDEPFSALDPIVRRDLQDTFVGLRDGGSVTLLFVTHDVAEALRVARRIVLIDDGAI
                     VADLTAGDFVRSAHPLIKRFLDAARIPEAS"
BASE COUNT         1309 a         2134 c         2317 g         1259 t
ORIGIN      
        1 gttctgcgcc accgcggcct tcacgagcgc cttgaatgcg cgcgcgtcga ctttctctcc
       61 ttcgtggatg tcgatcgccc gtcgcatgtt gccttccagg ctggaattga acagatgcga
      121 tgggtctggg agactggcgc cctgggcgaa ggtgagcttc accgccttcg tgtacatctc
      181 cccggtgcag acgatcccgt tgtgcgacca gaccgggacc cccgccgcat tggacggctt
      241 cttccatttc cgctcctcga tcatctcggg gtcggcctcc aggatcagcg cccgcatccg
      301 gtccagggtt tcctcgcgcc agtcccccgg gtcgtggatc cgtttgtcga ggagcgggga
      361 ggcgggtttc ccggtcgctt gctcgcggct cttcatgctc ctgcccccca tgtgagctgc
      421 acgagctgga tcaaattgcc aagggtgtcg tccagcaccg cgatgatgga gcccgtcgtg
      481 cgcgtgaccg gcacggtgaa cgtcactccc ttactggcca atcgatcgtg ctccgcctgt
      541 acgtccttca ccagaaactg agccgcgggc cggctctggt tgcggagcgc ttcctggtag
      601 gctttgccgg ccgggttggc attggactcc agcacgagct ccatgccatc cgggtcctcc
      661 ggcgaagcaa tcgtgagcca gcggtagttc ccattcgtga aatcggcctt cttctgaaag
      721 ccaagcgtct ccgtatagaa ctgcagcgcc ttctcctgat cgtcaacgta caaagtagtc
      781 cacttgatgc gcatcgcact ctcctgttcc tggtcaccct atgccggttc ttttccgcaa
      841 gagaagccag caaccaatac gccagaccga acgcgatcgg agcctgctca cgtgttctgg
      901 ccgattctag cgatggccgg gcgaagaggg aaccgtcaga caggcaaacg catcgaatgc
      961 gagcatttgt ccttgaagga tgggatgacg aaccggaact gcgatccctt tccaacctcg
     1021 ctcgtcacct cgatcgtccc ctgatgtgct tcaacgaatc ctttgacgat ggccagcccg
     1081 agtccggcgc cgccgcggcg gctcgatcga acctgccaga agcggtcgaa caaatgaggc
     1141 agttccgcgg ctggaatccc gggacctgtg tcggtgacgg agaatcgagc cttgtctcga
     1201 tcgccgtcga cagtcaaacg gacactcccg cgatcggtaa cgcgcagggc attgctgacg
     1261 aggttcgcga ggatttggat gaggcggttg cggtcggcgt acagcggcgg aagaacgccg
     1321 gaggcatcgg cctcgaaacg gatccctttc tctgcagctg ccggctcgaa catccgccgc
     1381 atttcctcaa tgacatcggc tgcatctaca tccgtgcgat cgacagtaaa gcctccgctc
     1441 tcgattcgcg cgacgtcgag caggtcgcct acgagcgact gcatccggcc gcatgaggtg
     1501 gaaatcatct cgatgagctc ttttcgttga acctcgtcgg tagtcagttg caggagcgcg
     1561 gcgctgctct tgatgatcgt cagcgggttt ctcaagtcgt gggcgacgat ggccaacact
     1621 tcgtccctcg atcgaatacc tttttcgagt gagatgatcg cttcggcctg acgatcgcgc
     1681 ggctgtgcgg gaacttcgaa gcgactgtcg cgcgacacgg gacccgtttc tttctgaata
     1741 gagatgctcg tttgaattga ggacattgga cggtcctcct cggagcagca tcgatcgtgg
     1801 ggccgctctt gtcagtgagc gctgaggttc cagcgcggtg accgagggca catcatcgtg
     1861 tgcgtgaggc tatcctcggc ctcgctgctc ccgaccggcc cagccacaga cggctttcat
     1921 ctgcgtacta tctgcgtcat ctgcggatcg ggctcacgcg tgcgctcctt gaccgcggtg
     1981 agccaccacg caatactcgg aaaatggaat cgcgtggcgg tcgccggttt gctctgcaga
     2041 aagcagcttt cggcgttctc gcgattcttg tcgtcctgct ttccgtcgat gtcctgggcg
     2101 tattcggtgc gggagaccgg aaggaatcgg ggaagcgcat tcctgatgcc acggtcgcgg
     2161 cgtggaacaa gctcgacgcc gacgcgaagc agcttctgga caaagagcac gacaacgtcg
     2221 tcgcggagat cggtcaccgc atcgagcacg agcatcttct ttttacgttg aagttcgcac
     2281 tggtcggagg gattctttat gggctgttgc agggcgcctt cggccgcggc gatacgcaca
     2341 tcgagaagac tgcattcgcc gcgttgatcg tctgggccgc ggtcatcgcg gccgcgatcg
     2401 tcgacctccg aatcatggcg aaccagagct tcctggttac tctcggcggt tggtcgcggc
     2461 aatacgaaga gctccgagtc ggtgccaacg gtgtcgatct tgcgtgggag gcattccttg
     2521 cgaacaatct gctcagtcaa cctcactatc cggcacttcg cgtgagcggg cagattctca
     2581 ccgcgctctt gtttgccttc agcggctcat tgttcttatt gtccaaagaa agcgaccacg
     2641 atccggcgat cgcactcgta agtggcagtt gtgcggttct gagcatcggc ctgatgaccg
     2701 tcgccgcgat cagtctccgg caacggggcg tggcggtctt gttgtatctg tcctttggta
     2761 ccgtggccgc tatcctcgcc ctcgttcttg cgcatacatc gaagagaaac tatcgggcag
     2821 agatcagaga ttagagatag gagagcagag atcagaggct atcagggcca gaggtccagg
     2881 aacgcctttc tggtctcttg tctctgatct cttgtctctt atcacttgcc caaggcgcgg
     2941 gccgatgatc cgatcgttcc cccgatcacc agcgcgcctg cggcgctggc ggcggccaga
     3001 gcggcggcgg cgagcatgct gatccggaat gcgcggatga aggcatcgtc gatggcggcg
     3061 gagacgcggg ctttctcggt ggcgtcgagt gttgccggag gcttcgcttc cgccaggcgc
     3121 aatcgctgtt ccgccatcgt ctgacgcagc gcgggggagg cgccgttcgc tgccagccgc
     3181 gcgtcgagag atcgggcgaa gatcatgatg gagagcgcac cgaagagcgc aatcgacagc
     3241 aggccggctg cgcgcgcgac ggtgttgttg atgccggacg cggcgccggc gtgtttctcc
     3301 tcgccgaaag acgtcatcac cgtcgttgtc agcggagcga cggtcgtagc catccctaat
     3361 ccgaggacga ggacggcggg gaagaacgtc gtccaatagg aaccgccgat gccgggacgg
     3421 gccagcagca ggaatccgca acaggccagg agagggccga cgatcagcgg cagccgcggt
     3481 ccgaagcggt cggcgagtcc gcctgtccat cgcgagagga tcgacatcgt cgccacgaat
     3541 gggagaaacg cccctcccgc ctgcgccggc gagtagtgct gcacctggat caggttgaaa
     3601 gggagcagaa aggtcaccgt cgaaagcgcg gcgtacagca gcagcgtcag cagattcgcg
     3661 ccgctgaatg cgcgggaacg gaacagcgtg agcggcatga tcggatcgtc gatccgccgt
     3721 tcgaggacaa tgaaggctat gaacgcaatc gtcccgaaca gcagcggccc gatgacgaga
     3781 gcgcttcgca ttccgcgcga tggtgcttcg atcattccga acgtgagcgc cgcgagcgcg
     3841 aacgtcacga gaagggtccc gccccaatcg atccgcccgg cgttcttcgt tttctgatcc
     3901 ggcggcatag cgcgccatac gatcaggagt gtgatcgctg cgatcggaag attgatgaag
     3961 aagacagcgc gccaggacac cgcttgcacc agccatccgc cgaagagcgg gccggcgatg
     4021 acggcaagcg aggtgagtga cgaccaggtg ccgatggcgc ggccgcgctc actggccggg
     4081 aacgtcgcgg tgatgatcgc caggctgccc ggaacgagga gcgccgcacc gagcccctga
     4141 aatgcgcgcg ccgcgatcag ttggtgaggg ttgaccgcgg ccccgcaaac ggccgacgcc
     4201 actgcgaaca tggcgatgcc gattgcgaag actttgcggc gtccgaggtg atcgccgagc
     4261 ccgccgccgg cgagcatgag cgaggagagc acgagcaggt aggcgtcgac gatccactgc
     4321 gtatcgctga ccgtcgcgcc catcgaacgc tgcagcaccg gaatggcgat gttgacgacc
     4381 gtcccgtcga tgaaggccat gcaggagccg aggatggcgg ccgccagcgt ccaggtgcgc
     4441 gcgcgtgaag agatcacttt tctttcagcc atgttctcgc cacctcggac gggctccgtt
     4501 tgtcgcggtc gatggccaga ttcaggcggc gcatggccgt cgcatcgaga ctacccgcga
     4561 gggaggccag cgccgccggg ccggccgcgc actttgtccc gaggtcacga cgcacaacca
     4621 gtgccgcgtc gtagggcgga aagaagtgcc ggtcgtcggc caggaccgtg agaccgagtg
     4681 cgtcgatgag gccgtcggtg gagttgcctg cgatcaggtc gatctcgccc gcggcaagcg
     4741 cgcgatacgt cagtcccaaa tccatcgttc gcggcgcctc ggcgaaacgg agtccgtaat
     4801 gctgcagcag tccgttccat ccatcgggcc gttcgacgaa ctcgtagccg aagcccggac
     4861 ggaatgtcgc gctgaccttg ccgaggtcgg agatggtctt cagcgagagc cgttgcgcat
     4921 cgctgctgcg cacgatcatg gcgaaggtgt tctcgaaccc gagggccggc ccccacgaga
     4981 gtccgcgcgc gccgtagtca gccgcaactt tcttcattac cgccgcgcga tcgcgcatcg
     5041 cgtcgtgatg gaggatggcg gtgagcgcag tcccggagta ctcgggatag acgtcgagcg
     5101 ctcccgcggt gatcgctttg tcgcagacga gcgtgccgcc gagatcgagt ttgcgttcca
     5161 ccgagcagcc ggcgcgctcg agccgttgcg ccacgatctc gccgaggagt acggactcgg
     5221 tgaaattctt ggagccgacg acgatcggtt tctgtttcgc gcaggcggcg agaagaagaa
     5281 tggcgatcga taagagaaca cgggcgcggg tggggggagt cgggagtcgg gggtcggagg
     5341 tcggggcgtt cggtcgacga cgctcgaaag cgacggaggg ggtcgggagt ggggggtggg
     5401 gggtggggag tgggagaaga gaggccacgg ttttcctacg gctgccgaca ccgacgaagg
     5461 gggtcgggag tgggggatgg ggagtggcga gtgggagaag agcgacctcg gttcttctcc
     5521 gacacccgac tcccgacacg cgactcccga cgccaaccat gtccatattg tcgcaggatc
     5581 agacgcccag tcgtctggca gccagacgct ccagggcgcc gaagagcaga tcggcagcga
     5641 cggcgagcgc cgcggcgggg agagcgccgg cgaggatccg cgtgctgtcg accatggcca
     5701 cgccgcggaa gatcaacgtg cccagcccgc cgccgcccac cgccgcggca atcgtcgcga
     5761 cgccgatcgc gatcaccgtg gcgatgcgaa tgcctccgaa gatcgaaggc agggcgagag
     5821 gaagatcgat cctcatcaat cgctgccgct cggtcattcc aagcgcggtg gccgcctcgc
     5881 ggatcaatgg gtcgacgccg gcaattccgg tcacggtgtt ccggacgacc ggcaggagtg
     5941 agtaaagaat gagcgcgacg atcgcggtgc gaataccgat gccgccgatg aaaggcaacg
     6001 gaatgaggaa cccgaagagt gccagagatg gaacggtctg aatgatgtcg atcaggcgga
     6061 ggacgatgcg tccggagcgc ggattgcgga tgcaggcgat gccgatggga atccccgcgg
     6121 atgtggccac gagcatggcc actccgacga tgagcaggtg ctgcgctgtc gcgtgaagga
     6181 tctcggcggt caggtcgctc atgacgcctc cgggatgcgc gcggcgtcga ggaagcgttt
     6241 gatgagcgga tgcgcggatc gaacgaagtc gcctgccgtc agatccgcga cgatcgctcc
     6301 gtcgtcgatc agcacgattc tgcgagccac gcgaagcgct tccgcaacat cgtgcgtgac
     6361 gaacagcaac gtgacgctgc cgccatcgcg cagcccaacg aaggtgtcct gaagatcgcg
     6421 gcggacaatc ggatcgagcg cgctgaatgg ctcgtcgcag agcagcagcg gcggatcggc
     6481 aaccatggcg cgggcgattc cgacacgctg ctgttcccct cccgaaagcg agcgtgggaa
     6541 acgtgcggca aaacgatcga acggcaagtc caggcgttgg agcaatgccg cactcgcttg
     6601 ccggatgcgc tcgtccggcc agccgaggag gcgcggaacg gtggccacat tctcgagcac
     6661 cgtccgatga ggaaagagag caggcgcctg caggatcgtt ccgatgcgcc gccgccgctc
     6721 ggccagtccc ggttcaccca gcggcaagcc atcgatgaca accgctccag tactcggagc
     6781 ggccagcccg ttgagaagcc ggagcagcgt ggttttgccc gcgccactcc tgccaatcac
     6841 cgccaccgat tcgcccggat cgatacgcaa cgagacatcg cgcacgatgg cattgccgtt
     6901 cgatccggcc acagtcgcgc gatcgatgac tacagaagag cccacgcggc gattgtatgg
     6961 gggtcaggtc taaagtctaa agtgccgaag acacttgatc gtctttagcg acgatcctc
//
LOCUS       DATLVW010000089         8036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_336444, whole genome shotgun sequence.
ACCESSION   DATLVW010000089 DATLVW010000000
VERSION     DATLVW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336444"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..923)
                     /locus_tag="VNN25_03010"
     CDS             complement(240..923)
                     /locus_tag="VNN25_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXH90525.1"
                     /translation="MLIRKFFAVTLCVSALAFPAAAARRAPATDSLDEVIRKVQLAQS
                     NTNTLQADFRQEKTLALLSNTEVSTGRFVYSKPNNVLWTYDAPKRVTMLIANGTLTTY
                     YPDLRKAERIEVKQYQDRIFKYMGASGAIDELGAWFNFTFTNTSDKPYFVLDLDPKSK
                     TVAKRVRHLRIYIDRKTFLTTQFEYTEGDGDKTRYEFTNVKVNAPIESSRFSLQLPSS
                     VRVESMKLQ"
     gene            1000..1863
                     /locus_tag="VNN25_03015"
     CDS             1000..1863
                     /locus_tag="VNN25_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90526.1"
                     /translation="MKKNLQAIAILFTVVLTAASAPSASAAIEYEFRQTMSSDLESIP
                     SSDCAGRAILDGDRSRVEFLSGNTYPIGTYIIATNGSRTMTFVDPSRKSYVEVNAASV
                     ASSIGARKITIANKKTDLTKMPDQITIAGFPTEHYRLVMSYDITVNFGTLPLTQSVKT
                     TIDKWTTTAFGDVGDTFLASGALHTGNPDLDAIVSAENTNIKGFALRQVVSVTTINNR
                     AAANADSQLHINRTVTQTSELVVTSIQPTAKVSLATFVVPLTYHKAGPVQDDTQKSPM
                     QTLSMEPSGQE"
     gene            2183..3490
                     /locus_tag="VNN25_03020"
     CDS             2183..3490
                     /locus_tag="VNN25_03020"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXH90527.1"
                     /translation="MRRFAHRPRVYAAIALPLAIPFVLAAVTTDGALTQPPIVNSAAM
                     IAVPPLRYADLTAPTAASLPEHSVVLTVEDGDTLESVLLAGGLGRADVGALNSEFGKT
                     IDLRRLRPGHLLRFHYDTRGSVDSVEMKVTGWGEIDAIRNASGFTITPRQAQQREVET
                     TVSAAIDSSLWDAVRGAGEGPGLVSELADVFQWDIDFFALQKGDSFSVVVNKRYAGSD
                     LVGYGPIVAARFSYEGKTYEAFRCQTPDGRAGYYARNGSPLRKQFLRAPLQFSRITSG
                     FSKSRYHPLLHIFRPHHGVDYGAPVGTPVMTTADGVVMSATYNHGEGNYVRIRHSARI
                     ETSYLHLSRFAKGIKPGKSVSQGEVIGYVGATGLATGPHLDYRVSDNGTWLNPLQLKS
                     ITSDPLRGDSLGAFRTSVAALSPKLDTPAQQLIAFAAKRRALF"
     gene            3521..5374
                     /locus_tag="VNN25_03025"
     CDS             3521..5374
                     /locus_tag="VNN25_03025"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90528.1"
                     /translation="MKKVIVRAPVRADLAGGTLDLWPLYLFHPGSRTVNVAISYHAES
                     EVEETGDGAIDIHLTDQHYEQRYQSIHELAADPKAALIHRALEHFHLTGIRITTRTDA
                     PRGSGLGGSSALTITLVRALSELAGAPIEGDDLIALVRDLETRLLGVPAGIQDYYPPV
                     YGGLMAIHLNPGAVLRHPIALPVERLAEHMLLHYTGVAHFSGTNNWDIYKRQIEGKKK
                     IHKGLDRIAESAIEMEKALESGDMEAAGVALGHEWANRKALIEGISTPEIESAIAAAV
                     GAGAWSGKVCGAGGGGCIVFLMSPERRESVREALSHVPGQVIDAHPVAHGLIIERSDD
                     VPKARPRFKMRSSPSLEQLYVFGGSGAYKPHLLAEAIITQSEPRSGLHVDIVKSFVAP
                     MHASDGTVRWSEARTIEPEKLDIRAVPDPNHRVDDGLKPEMLMQGALLSEDAFRDYVS
                     GTAKLTLLHNPEFALYSQQDEGRSAFLKRCAEEAKLRIELQQEKLESTFRRRIDQVKE
                     LSERDQREREASDETTESERGPEVNVAWGQTIYNITSGKPAAVDESPHSLREDDYLSK
                     IRQIQRSWDRELQVIREELEAKAREIEEIVITPSAKNIEITKYLILWAAGL"
     gene            complement(5379..5648)
                     /locus_tag="VNN25_03030"
     CDS             complement(5379..5648)
                     /locus_tag="VNN25_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90529.1"
                     /translation="MEFRSGTLMKIKSKTYEAVAEYWNTHEMSDENSEPVELEVDLQR
                     PATYFPIEKRLADQLKTAAAAQGVSSKALLNRWVQQKIEEEVASK"
     gene            5699..5775
                     /locus_tag="VNN25_03035"
     tRNA            5699..5775
                     /locus_tag="VNN25_03035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5733..5735,aa:Pro,seq:ggg)
     gene            5946..>8036
                     /locus_tag="VNN25_03040"
     CDS             5946..>8036
                     /locus_tag="VNN25_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90530.1"
                     /translation="MNANIQDELDRWTNRIRQIDRILGPAISSLIDLARWNVDVGGAS
                     PQSLHPFAGWVREPFYFPAWLTDDREPVLQDRINLTTALCVRVLQRLWLIRRRFPALE
                     KKLEGLQELVDGKGRGRDLGEFLADAFLPPTDQEDAYRAYIRNANLMSRWYPFSTANI
                     LWVLLRGGESKAYTGAGVLALFAMIWSLNGTEGAPGAAIGMLPASAYVTNSCLTAIRS
                     LTGACRRRAEIVKEIAERIDEIDQLVTHTRSSAQLPFHIEALLSLLYAFRRVAITGSA
                     FEEELTAIDNISRNVGKNGKPDLIWAQVRKELRTIVDNIAASGDELTKDFNSVRTVLT
                     EIGDSVDAAYLSDDPTDAIRRLEELGISVPAVGFTRSEARKAFWDMRAEAVRIAQKAC
                     RSAMEAFEVSVKELHPDASSEGSLANAFRSIQRGNEAVIAAIGETVDKSQHWCASVVM
                     REIANASSGNWTEFDPAELAGALESATLARAIESPQLIADAVVKALDGLCGRGAWIAG
                     QPFMLDETFSMAAVAPAADVIFIVTRVLRAHPDITAADDQLLSFVDWIEASRTEFLIS
                     QLSQTALPKEIRLCGWVSERQARPRRIDLWNTAFTIDALLELRSLFEDRLWALCERRF
                     TIVEDIGPLGELEAADLDTPHRSRLHRRLADMARATSSAGYRHADYSLVLHGPPGSAK
                     TKVVGALASELWRMN"
BASE COUNT         1615 a         2583 c         2319 g         1519 t
ORIGIN      
        1 aatcttaaaa cgacccctcc gaatcacaga tcgatctctt ttctcgagat cgcgatctcc
       61 ttttttgaga tcgctacccc cccgaatcct aaaacgaccc ctccaaactc accatcgagg
      121 tgcctccttt tgaggacaat cgactacgac tcgatgagga agagtgttcc ctcggcgcgc
      181 aaggcagatt caacgcgcga agcgcaacca accgacaacc gacaacgcgc gcagcgcaat
      241 tactgcagtt tcatcgattc cacccgcacg ctgctcggca gctggagtga aaagcggctc
      301 gactcaatcg gggcattgac cttcacgttc gtaaactcgt agcgcgtctt gtccccgtcg
      361 ccttcggtgt actcgaactg ggtcgtgagg aaggtcttcc tgtcgatgta gattcggagg
      421 tggcgcacgc gcttcgcgac cgtcttgctc ttcgggtcga ggtcgaggac gaagtagggc
      481 ttgtcggagg tgttcgtgaa cgtgaagttg aaccaggcgc ccaactcatc gatcgcgccg
      541 ctggctccca tgtacttgaa gatgcggtcc tggtactgct tcacctcgat ccgttcggct
      601 ttgcgaaggt cgggatagta ggtcgtgagc gtgccgttgg cgatgagcat ggtcacccgc
      661 ttcggcgcgt cgtaggtcca gagaacgttg ttcggtttcg aatagacgaa ccgcccggtc
      721 gagacctcgg tgttggagag aagtgcgagg gtcttttcct gacgaaaatc cgcctgcagc
      781 gtgttcgtgt tcgactgcgc gagctgcacc ttacgaatga cttcgtcgag cgagtcggta
      841 gccggcgccc ggcgggccgc cgcggctggg aaggccagag ctgacacgca gagggtgacc
      901 gcgaaaaact tcctgatcaa catgggctgc cccaacgccc cgcccgaaag tcgtatttca
      961 ggcgatgcga gttatactca gtgaaaactg aggttattga tgaaaaagaa cttgcaggca
     1021 attgccatcc tgttcaccgt cgtgctcacc gccgcaagtg ccccgtctgc ttccgccgcc
     1081 atcgagtacg agttccggca aacgatgtcc tcggatctcg aatcgatccc ctcaagcgat
     1141 tgcgccgggc gtgccatcct cgacggcgac cgctcccgcg tggagtttct ctcgggcaac
     1201 acctacccga tcggcaccta catcatcgcc accaacggct cgcgaaccat gaccttcgtg
     1261 gacccctcgc ggaagtccta cgtggaggtc aatgccgcca gcgtcgccag ctccatcggc
     1321 gcccgcaaga tcaccatcgc caacaagaag accgatctca ccaagatgcc tgaccagatc
     1381 accatcgccg gctttcccac cgagcactac cgcctggtca tgagttacga catcaccgtc
     1441 aatttcggca ctctcccact cacgcagagc gtgaagacga cgatcgacaa gtggacgacc
     1501 accgccttcg gcgacgtcgg cgacacgttc ctcgccagcg gggcgttgca caccggaaac
     1561 cccgacctcg acgcgatcgt ctcggcggaa aacacgaaca tcaaagggtt tgcgctcagg
     1621 caggtcgtca gcgtcacgac gatcaacaat cgcgccgccg ccaacgccga ctcgcagttg
     1681 cacatcaacc gcacggtgac ccagaccagc gaactggtcg tgacgtcgat tcaaccgacc
     1741 gcaaaggtgt cgttggcgac cttcgtcgtt cctctgacct accacaaggc cggcccggtc
     1801 caggacgata cgcagaaatc gccgatgcag acgttgtcga tggagccgag tggacaggag
     1861 tgacggaaag cagagatcag agactagaga tcagagatca ggggctgaca gacaagactt
     1921 tgtggaaccc ggacctgtat ctctgatctc cacccggacc tttatctctg atctccaaac
     1981 tctggtaacc ctgatctcta gtctctgatc tctggtaacc ctaatctctg atctccagtc
     2041 tctggtaacc cctgatctct agtctctggt ctctaatctc tgtagtcagg cggtcgggag
     2101 ggcgtgcatg gtacgatccg gtcagttcgt ccgcggaacc ggttcaagat cgatgaaaag
     2161 atcgttttct atcgctgagg tcgtgcgccg tttcgcacac cggccccgag tgtacgccgc
     2221 gatcgctttg cctctcgcga tccccttcgt cctcgctgcc gtcaccaccg acggtgcgct
     2281 cacgcagccg ccgatcgtga acagcgcggc aatgattgcc gttccccctc tccgctatgc
     2341 cgatctgacc gccccaacgg cggcctcgct tcccgagcat tccgtcgtcc tgaccgtcga
     2401 agacggcgac acgctcgaat ccgtcctcct cgccggcgga cttgggcgtg ccgacgtcgg
     2461 cgcgctcaat tccgagttcg gcaagaccat cgacctccgc cgcctgcgcc ccgggcacct
     2521 ccttcgattt cactacgaca cccgcggaag cgtggacagc gtcgagatga aggtcaccgg
     2581 ctggggtgag atcgacgcga tccgcaatgc ctcgggcttc acgatcacac ctcggcaggc
     2641 gcaacagcgc gaggtcgaaa ccaccgtctc cgccgccatc gactcatcgc tgtgggatgc
     2701 cgttcgcggc gccggcgaag ggccggggct cgtttccgag ctggccgatg ttttccaatg
     2761 ggacatcgac ttcttcgcgc tgcagaaggg cgactcgttc agcgtcgtgg tcaacaagcg
     2821 ctatgcaggc tctgatctcg tcggatacgg cccgatcgtc gcggcacgct tttcctacga
     2881 ggggaaaacg tacgaagcct tccgctgcca gacccccgat ggccgcgccg gctactacgc
     2941 ccgcaacggc agtccgcttc gcaagcaatt cctccgcgcg cctctgcaat tcagccggat
     3001 cacctcaggc ttctcgaaga gccgctacca tcctctgctg cacatcttcc ggccgcatca
     3061 tggcgtcgac tacggcgcgc cggtcgggac gccggtgatg acgaccgctg acggcgtggt
     3121 gatgtccgcc acgtacaacc acggcgaggg aaactacgtt cgcatccgtc acagcgcaag
     3181 gatcgagacg tcgtacctcc acctttcgcg tttcgcaaaa gggatcaagc cgggcaagag
     3241 cgtgagccag ggcgaggtga tcggctacgt cggcgctact ggcctcgcga ccggaccgca
     3301 tctcgactac cgcgtgtccg acaacggcac gtggctcaat cctctgcagc tcaaatcgat
     3361 cacatccgat ccgcttcgcg gcgactccct cggcgccttt cgcaccagcg ttgctgctct
     3421 gagtcccaaa ctggacactc cggcacagca actcatcgcc ttcgcggcga agcggcgggc
     3481 acttttctag cagtcgcacg gatcaacgaa cgcccacccc atgaaaaagg tcattgtccg
     3541 cgcgccggtg cgcgccgatc tcgctggcgg taccctcgat ctctggccgc tctacctctt
     3601 tcatcccggc tcgcgcaccg tcaacgtcgc catctcgtac cacgccgaat ccgaggttga
     3661 agagaccggc gacggcgcga tcgacattca cctgacggat cagcattacg agcagcgcta
     3721 ccagtcgatc cacgagcttg ccgccgatcc gaaagcggcg ctgatccatc gtgccctcga
     3781 acactttcac ctcaccggaa tccgtatcac cacccgcacc gacgccccgc gcggctccgg
     3841 tctcggcggc tcttccgcgc tgacgatcac gctcgtccgc gcgctctcgg agctcgccgg
     3901 tgcgccgatc gagggcgacg acctgatcgc cctcgtccgc gatctcgaaa cgcgccttct
     3961 gggcgtgccg gccggcattc aggattacta cccgccggtc tacggcggcc tcatggccat
     4021 ccacctcaac ccgggcgccg tcctgcgaca tccgatcgct ctgccagtgg aacgtctggc
     4081 cgagcacatg ctcctgcact acaccggcgt cgcgcatttc tccggcacga acaactggga
     4141 catctacaaa cgccagatcg agggcaagaa gaagattcac aaaggcctcg accgcatcgc
     4201 cgagagcgcc atcgagatgg agaaggccct cgagagcggc gacatggagg cagctggcgt
     4261 cgctctcggg cacgaatggg cgaaccggaa agcgctgatc gaagggatct ccacgccgga
     4321 gatcgagtcc gcgatcgcag cggctgtcgg agccggcgcg tggtcaggga aagtctgcgg
     4381 tgccggcggc ggtggttgca tcgtcttcct gatgtcgccg gaacgccgcg aatcggtgcg
     4441 cgaagccttg tcgcacgttc ccggacaggt catcgacgct catccggtcg cacacggtct
     4501 gatcatcgag cgcagcgacg atgtgccgaa agcgcggccc cgcttcaaga tgcgcagctc
     4561 gccatcgctc gaacagctct acgtgttcgg cggcagcggc gcgtacaaac cgcacctgct
     4621 ggcggaagcg atcatcacgc agagcgagcc gcgcagcggc ttgcacgtcg acatcgtcaa
     4681 gagcttcgtg gcgccgatgc acgccagcga cggcacggtt cggtggtccg aggcgcgaac
     4741 gatcgagccg gaaaaactcg acatccgcgc ggtacccgat cccaaccacc gcgtcgatga
     4801 cggtctgaaa ccggagatgc tcatgcaggg agcgcttctg tcggaggacg cgttccgcga
     4861 ctacgtgagc ggcacggcca agctgacgct cctgcacaac cccgagttcg ctctttactc
     4921 gcagcaggac gaagggcgct cggcgttcct gaagcgttgc gcagaagagg caaaacttcg
     4981 catcgagctg cagcaggaga aactggagag cacgttccgc cgccgcatcg accaggtgaa
     5041 agagctctcc gagcgcgatc agcgcgaacg cgaagccagc gacgaaacga ccgaaagcga
     5101 gcgcggcccc gaggtcaacg tcgcctgggg ccagacgatc tacaacatca ccagcggcaa
     5161 acccgccgcc gtcgacgaat cgccccactc cctgcgcgaa gacgactacc tctccaaaat
     5221 ccgccagatc caacgatcct gggacagaga gctccaggtc atccgcgaag aactggaagc
     5281 caaagcccgc gagatcgaag agatcgtgat cacgccgagt gcgaagaaca tcgagatcac
     5341 caagtacctg atcctctggg cggcggggct gtaggcagct acttcgacgc gacctcctct
     5401 tcgatcttct gctgcaccca ccgattgaga agggccttcg aggaaactcc ctgcgctgcg
     5461 gccgcggtct tcagttgatc ggcgagacgt ttctcgatgg gaaagtaggt cgcaggccgc
     5521 tgcagatcga cttccaactc gaccggctcg gagttctcgt ccgacatctc gtgcgtgttc
     5581 cagtactcgg cgaccgcttc gtaggttttg ctcttgatct tcatcagcgt gccgcttcgg
     5641 aattccaagt cagcgttttc gttacgtccg gacgcgcatg ctacgatgcg caccgagtcg
     5701 ggcagtagcg cagcctggta gcgtacctga atggggttca ggtggtcggg ggttcaaatc
     5761 ccccctgccc gaccaaacaa ctaaccccaa cgggccgtta tacgccgaag aatctcgatc
     5821 agctgcgact gttcggggac ctgtcctact cacgcccatt tccggttccc tatcgtgggg
     5881 tagggaaagg gtactattcc gacactccct gagtgtggtg cgaggatcag accggggggg
     5941 cgaagttgaa cgcaaatatt caagatgaac tcgatcgttg gacgaatcga attcggcaga
     6001 tcgaccgcat ccttgggccg gcgatttcta gcctcatcga tttggcccgg tggaacgtcg
     6061 acgtgggggg ggcttctccg caatctctgc atccgttcgc tggttgggtt cgcgagccat
     6121 tctactttcc agcttggctg acagacgacc gtgagcctgt gcttcaggac aggatcaacc
     6181 tcacgacagc gttgtgcgtg cgagtcctgc aacgtttgtg gttgataagg cgccggttcc
     6241 ctgctttgga aaagaagctc gaaggccttc aagaacttgt tgatggaaag ggtcgcggcc
     6301 gtgaccttgg ggagtttctc gctgacgcct tcctcccgcc cacagaccaa gaagacgcgt
     6361 atcgagcgta tatacggaac gccaacctca tgtcgcgttg gtatcccttt tcaaccgcga
     6421 acattctttg ggtcctcttg aggggcgggg agtcgaaggc gtacacgggc gccggagtgt
     6481 tggcgctctt cgcgatgatc tggtcgctga atggaaccga gggggcgccc ggcgccgcta
     6541 tcggcatgtt gccagcatcc gcatacgtca ccaactcctg tctcacggct attcgttcgt
     6601 tgacgggtgc ctgccgcagg cgtgctgaaa tcgtcaagga gatcgccgag cggattgacg
     6661 agattgacca gctcgtgact cacacgcggt catcggcaca acttcccttt cacatcgagg
     6721 ccctacttag tctcctatac gcattccgaa gagtagcaat caccgggtcg gccttcgaag
     6781 aagagctcac agcgatagac aacatcagta gaaacgtcgg gaagaacggt aagccggatc
     6841 tcatttgggc gcaagtacgg aaggaactcc ggaccatcgt cgacaacatt gccgcctcgg
     6901 gcgacgagtt gactaaggac ttcaattcag tgaggacagt gctcaccgaa attggcgaca
     6961 gcgttgatgc cgcctacttg agcgatgatc cgacagacgc aatcaggcgc ctcgaagaac
     7021 tcgggatcag cgttccagca gtcggcttca cgcgatcgga ggcccggaag gctttctggg
     7081 acatgcgggc agaggctgtc cgcattgccc agaaggcatg tcggtcggcg atggaagcct
     7141 tcgaggtttc ggtgaaggag cttcatcccg acgcatccag cgaaggcagt ctcgcaaatg
     7201 ccttccgatc gattcaaaga ggtaacgaag ccgtcatcgc cgccatcgga gagaccgtcg
     7261 acaagtcaca acattggtgt gctagcgtcg tgatgcgcga gatagccaac gcgagtagcg
     7321 gtaactggac agagttcgac ccggccgaac tggctggcgc gctggagagc gccaccttgg
     7381 cgagggcgat cgaaagccct caactgatcg cggacgcggt cgtgaaggcg cttgatggtc
     7441 tatgcggacg aggcgcatgg atcgcgggtc agccgttcat gctggacgaa acgttttcga
     7501 tggcggctgt cgccccggct gccgacgtca tcttcatcgt cacccgcgtg ctgcgcgccc
     7561 atcccgacat taccgctgcc gacgatcaac tgctctcatt cgtcgactgg atcgaggcct
     7621 cccgaaccga gttcttaatc agtcagttat ctcagaccgc tttaccgaag gagatccgtc
     7681 tctgcggctg ggtttcggag cgccaagccc gtccccgcag aatcgacctt tggaacaccg
     7741 ctttcactat cgacgcgctg ctcgaactgc gtagcctttt cgaagatcgg ctgtgggcac
     7801 tgtgtgaacg acgcttcaca atcgtcgagg acatcggtcc cctcggtgaa cttgaagccg
     7861 ccgatctgga cacgccgcac cgctcacgcc tccaccgccg gctggcagat atggctcgcg
     7921 ccaccagctc cgccggatat cgccatgccg attactcact ggtgctccac ggaccgcctg
     7981 gatcagcgaa aaccaaggtc gtcggcgcgc tcgctagcga actttggagg atgaac
//
LOCUS       DATLVW010000090         7651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_129308, whole genome shotgun sequence.
ACCESSION   DATLVW010000090 DATLVW010000000
VERSION     DATLVW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129308"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VNN25_03045"
     CDS             complement(<1..279)
                     /locus_tag="VNN25_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH90531.1"
                     /translation="MSEARRLHRYNYADYVALEMFSTSKHEFLDGEIYAMVAGSEEHS
                     ALSAAMVHALGNTVGDRPCRVHTSDLRIFVESVGLATFPDCSVVCGPLQ"
     gene            382..711
                     /locus_tag="VNN25_03050"
     CDS             382..711
                     /locus_tag="VNN25_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90532.1"
                     /translation="MSDDHDPLAEIERLDRFFDGDEEGERPIDVLRASGVSIPDERDL
                     DDATLHARLWELIRHRRPDRFRRSIRILEEDEETGSGLEQSPPMGGDNLSLPLLWSSR
                     GLKAGKD"
     gene            716..1411
                     /locus_tag="VNN25_03055"
     CDS             716..1411
                     /locus_tag="VNN25_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90533.1"
                     /translation="MSTSVSDGYPEVTLLLRHWSNGGSALPAVKKLTECGYGDYAAAV
                     ARLALRAPDCPDRESLEAAIAQIANVSDGWVRAIEEFANHPSEERWDELMRFVPVEEH
                     YQRFRYTLALLLSLHCDGNILFQCATKIGMTSDAFDLARSGTVDPEVIEARGEGSAAR
                     PAWLGLAAQAAFARNDRWSTLRYLREASQYGESSVLSWSSISEIRSEADEEPQRRIGP
                     RRRAARVEPFCVS"
     gene            complement(1438..2481)
                     /locus_tag="VNN25_03060"
     CDS             complement(1438..2481)
                     /locus_tag="VNN25_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXH90534.1"
                     /translation="MRRILRFLLRSLLVVIALCGVLAVLFVYLVYTPPSDVPHLSGTV
                     TKGSIIVDGLPRTYRTYAPRGLAKGASLVVVMHGSGENGGQIRVGTGYGFDRLADAHG
                     FAVVYPDAYQGYWDVCSVVGAVSVKGLKIDDVRFLTGLADKLVAEIGVDPSRVFAVGG
                     SRGGSMALRLALEAPSRFRAVAAVSASVPTPENFQCKPAGHGTSSVMIMNGTEDPIVP
                     FNGGDVNLLGFFYRNGKVRSSRGSAQYFADLNNIAGTPETRETRVADGVRVEQVLWRN
                     HSNVEVELVAIHGGGHGIPQPYRRRPRILGPSVKEPNGPAMIWSFFDRQRAALSAKTK
                     GNHRDTESTEGSL"
     gene            complement(2553..5894)
                     /locus_tag="VNN25_03065"
     CDS             complement(2553..5894)
                     /locus_tag="VNN25_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HXH90535.1"
                     /translation="MAVTNRQRVDQALELLNRGLLPYVEREMKSVYGAAKWEKQAILA
                     LGDRPLLKAGNWDTPALLSVMIGEWQNVFKRGLGQTERSIVGEMRDIRNRWAHQEVFT
                     SDDTYRVFDNVHRLLTAIGAKEAIELDRQKQELLRIRFEEQAKKETVKAAVAAVETGV
                     SPSSGLKPWREVITPHPDVHSGRYQQAEFAADLGQVHRNEGSDEYRDPSQFFQRTYIT
                     LGLRTLLSGALQRLSGMGGDPIVELQTNFGGGKTHSMIALYHFFSGVSADKLRDIEPV
                     LAEAKLEKLPKANRAVLVGQELPLAEPIKKKDGTVVRTMWGELAWQLLKKEGYELVAE
                     ADRKGVSPGSTALRELFTLAEPCLVLIDEWVAHARQLVGKDDLPAGTFDSNFSFAQEL
                     TEAARACKKTLVVASVPASDDQHERASPWVEDIEVGGEAGRTALKRLENVFGRMQSPW
                     RPASAEESFEIVRRRLFQPMTADVYKHRDAVIRAFGDTYNTQKSEFPSGCSEGDYRRR
                     MESSYPLHPELFDRLYEDWSSLQRFQRTRGVLRLMAAVIHALWRDEDKSLLIMPASVT
                     LYDSATVNELMLYLDDALRPVVEREVDGTDSLPLGLDKENPNFGRLSAARRVARTIFM
                     GTAPRLRSATKGIEDKRIILGCVQPGEIVATFRDALRRLADRASHLYVNENRYWFDTS
                     PSINRTAQDRAEGWKADVVHKEIEDRLRVEAKTRGDFDRVQVIENASQSGDVPDERDV
                     RLVIIGPEAPHTKGSTSSAMTVAQTVLEQRGNSPRSYKNALVFLAAESKALDSLEQAV
                     RTWLAWKSIDAEKETMDSLSMFQRRQVETKLKDSESAVHARIPETYTWLLVPEQQFDE
                     KERKVGPLTWREVRVTGSDHLSVRASKKMVNDGMLIATHWAPVLLRMELDRIPLWRGD
                     HVSVKQLADDFATYVYLPRLRNADVLLNTISAGASAILWEKDTFAYADVYHEETKEYG
                     GLIARSAPPSVDMTGIVVRSEIARTQITRDTPVTPVDASNPTSPTQALSPGSELSAVP
                     SPEPSIPTRFWANLDVDALRMPREAGQIAEAVVAHLNGLVGAKVRISVEIEARVPDGI
                     PDSVQRTVSENCRTLKIDAFGFEEK"
     gene            complement(6116..6619)
                     /locus_tag="VNN25_03070"
     CDS             complement(6116..6619)
                     /locus_tag="VNN25_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90536.1"
                     /translation="MERSLNRMPDLDWLPPLVVLADSGGDWQRYVEVVYGFFKADFIA
                     SSPTYDGRRCACKRHPLIQGKEGTFWHLISEGGVEEDRLPDLRRCERIRWPRPMVEHS
                     PGKGVVSWRSSRPGDKKRIVIALEDFSYVVVLADRGKYLLLWTAYCVEQEHRRAKLRR
                     EYERDSA"
     gene            complement(6604..7299)
                     /locus_tag="VNN25_03075"
     CDS             complement(6604..7299)
                     /locus_tag="VNN25_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HXH90537.1"
                     /translation="MTGFNIYCDESCHLEHDTSKVMVLGAIWCPEEKVREIAVRMREI
                     KQKHGLPRWYEVKWTKVSPSKLGFYLELLDYFFDDDDLRFRAVIVPDKGSLRHADFGQ
                     DHDTFYYKMYYSLLKNILNPDDRYNIYLDIKDTRSHAKEQKLREILCTSLHDRDLEVI
                     ERLQSVRSCEVEQIQLADFLIGIVSYVNRGISGSAAKQTLVERMRQRSGYRLTLSSLV
                     RATKVNIFRWNAA"
     gene            complement(7296..>7651)
                     /locus_tag="VNN25_03080"
     CDS             complement(7296..>7651)
                     /locus_tag="VNN25_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90538.1"
                     /translation="LRAAKGKVQLLGREQLDIDWDPVSDKRLTIWEVAQHLIQRLKSG
                     GEQDAADLLRKVGGMGEMARDLAYRLYSVCERKGWTEEAIAYNSLVVAWPEISRLAAD
                     SSNTSASAQGTMFKA"
BASE COUNT         1490 a         2383 c         2226 g         1552 t
ORIGIN      
        1 ttgcagcggg ccgcagacta ccgagcaatc gggaaacgtc gccaggccaa ccgattcgac
       61 aaagatccgc aggtccgagg tgtgcacgcg gcagggacga tcaccgacgg tgttaccgag
      121 cgcgtgaacc atcgcggctg acagcgcgga gtgctcctcg gaaccagcaa ccatcgcgta
      181 gatctcgccg tcgaggaact cgtgcttgct ggtggagaac atctcgagcg ccacgtagtc
      241 ggcgtagtta tagcgatgca gccgacgcgc ttcgctcatc cagcgattct aatccgactc
      301 ctcagaacac tctccactca gtagaaaaac ttgactctcc ctcaatcccg agggagcctc
      361 ggtgcgtgag gaggttcggc aatgtctgac gaccacgatc cgctcgcgga gatcgaacgg
      421 cttgaccggt ttttcgatgg cgatgaggag ggagaacgcc cgatcgacgt cctgcgcgcc
      481 agcggtgtat cgattccgga cgagcgcgat ctcgatgacg ccacgctgca tgcgagactc
      541 tgggagctca tacgtcatcg ccgccctgac agattccgcc gatccatccg aatactggaa
      601 gaagatgagg aaacgggatc cggacttgag caaagccctc caatgggggg ggacaatttg
      661 tccctcccct tgctttggag ttcgcgaggt ttgaaagccg gaaaggactg actggatgag
      721 cacttccgtc agcgacgggt atcccgaagt gacgctcctg ctgcgccact ggagcaacgg
      781 cggcagcgcg ttaccagctg tgaaaaagct gaccgagtgc ggctacgggg actacgcggc
      841 agccgtcgcc cgcctggcgt tgcgagcgcc ggattgcccc gatcgcgaaa gcttggaagc
      901 ggccatcgcg caaatcgcga acgtatccga cggctgggtg agagcgattg aggagtttgc
      961 gaatcatccg agcgaagagc gctgggatga gctgatgcgc ttcgtccccg tcgaagagca
     1021 ctatcagcgc ttccgttaca ccctcgcact tctcctgtcc cttcattgcg acggcaacat
     1081 tctctttcag tgcgccacga agatcggaat gacctcggac gcattcgatc tcgcccgaag
     1141 cggcacggtc gacccggaag tgatcgaagc gcgaggtgaa ggatcggcag cgcggcccgc
     1201 ctggttaggc ctcgccgccc aggccgcctt cgcgcgcaac gaccggtggt cgaccctccg
     1261 ctatctccgc gaggcgagcc aatacggaga gagctctgtc ctgtcatggt ccagcatctc
     1321 cgaaatccgg agcgaagccg atgaggagcc tcaacgacga attggaccgc gtcggcgtgc
     1381 cgcgcgtgta gagcctttct gtgtctcttg aaaccggtca ccacaaagac gcaaaggtca
     1441 cagagacccc tctgtgctct ctgtgtctct gtggttgcct tttgttttcg ccgacaacgc
     1501 cgcccgctgc cggtcgaaga aggaccagat catcgccggt ccgttcggtt ccttcaccga
     1561 tggtcccagt attcgcggac ggcgccggta tggctgcggg atgccatgtc caccgccgtg
     1621 aatggcgacg agctccacct caacattgga gtggttccgc cagagtacct gctcgacgcg
     1681 aaccccgtct gccacccggg tctcgcgcgt ctccggcgtg ccggcgatgt tgttgaggtc
     1741 cgcaaaatac tgcgctgacc ctcgcgacga tcggaccttg ccgttcctgt aaaagaagcc
     1801 gaggaggttc acatcacccc cattgaacgg gacgatggga tcctccgtac cgttcatgat
     1861 catgacggaa gacgtgccgt gtccagcagg cttgcactgg aaattctccg gcgtcggtac
     1921 gctcgccgaa accgccgcca ccgcgcgaaa ccgcgaagga gcttcaagcg ccagccgaag
     1981 cgccatgctg ccccctcggg aaccaccaac cgcaaacacg cggctcggat cgacaccgat
     2041 ctcggcgacg agcttgtccg ccaaccccgt cagaaacctc acgtcatcga tcttgagacc
     2101 cttcacgctg accgcaccaa caacgctgca cacatcccag taaccctggt acgcatcggg
     2161 ataaacgacc gcgaagccat gtgcatcagc aaggcgatcg aacccatacc cggtcccgac
     2221 tcgtatctgc ccgccgtttt ctcccgaccc gtgcatcacc accacaagcg acgctccctt
     2281 cgccagcccc cgcggcgcgt aggtccggta agtccgcggc agtccatcga caataatcga
     2341 ccccttggtc acggtgccgg acagatgggg aacgtcagaa gggggtgtgt agacaagata
     2401 gacgaacagc accgccagca cgccacacaa cgcaatcacg acgagcaggc ttcgaaggag
     2461 gaaacgtaga attcttctca tcgccatcga tctcgagagc ccgcggccgc cttaccgcgc
     2521 tagcgcgaac tagatccact ccgcaacggc gtctatttct cttcgaaccc gaatgcgtca
     2581 atcttcagcg tccggcagtt ctcgctgacc gttcgttgaa cactgtcggg aataccgtca
     2641 ggcaccctcg cctcgatctc gacactgatc ctcacctttg ctccaacaag gccattgagg
     2701 tgagcgacca cggcttctgc gatctggcca gcttcgcgag gcatgcgaag agcgtccaca
     2761 tcgaggttcg cccagaagcg agttgggatg cttggttccg gagaagggac ggcggacagt
     2821 tcgctaccag gagacagagc ctgcgtaggg gaggttggat tggaggcgtc gaccggcgta
     2881 acgggcgtat ccctcgtgat ctgagtgcgt gcgatttcgg atcgcacaac gatcccagtc
     2941 atgtcgaccg atggcggcgc acttcgggca atcagccctc catattcctt cgtctcctcg
     3001 tggtaaacgt cggcataggc aaaggtgtct ttctcccaga ggatcgctga cgcgcccgcg
     3061 gagatcgtgt tcaggagtac gtctgcgttc cgaagtcgag gcaagtaaac gtacgtcgca
     3121 aagtcatcag caagctgttt gacgctgacg tgatcgcctc gccagagcgg aatccgatcg
     3181 agctccatgc gcaggagaac aggtgcccag tgcgtcgcga tcagcatccc gtcgttgacc
     3241 atctttttcg acgctcggac tgagagatga tcgctgcctg tgacgcgcac ctcgcgccag
     3301 gtcagtggcc caaccttccg ttccttctca tcgaactgtt gctcggggac caggagccat
     3361 gtataggttt ccggaatgcg cgcatgcacg gcgctctcag agtccttcag cttggtttcg
     3421 acctgtcttc gctggaacat cgaaagagaa tccatcgtct ccttctctgc gtcgatcgac
     3481 ttccacgcaa gccaggttcg gaccgcctgc tccagcgaat cgagagcctt cgattcagcg
     3541 gcgagaaaaa cgagcgcgtt cttgtagctt cgcggcgagt tccctcgttg ttccaggact
     3601 gtctgcgcca cggtcatcgc cgagctggtg cttccttttg tatgcggagc ctcggggccg
     3661 atgatcacaa gccgcacatc gcgctcgtcc ggcacgtcgc cgctctgcga ggcgttctcg
     3721 atcacctgga cgcggtcgaa atctccacgc gtctttgctt cgacgcgaag gcggtcttcg
     3781 atctccttgt gaacgacgtc agccttccaa ccctcggcgc gatcctgcgc agtccggttg
     3841 atactcggcg acgtatcgaa ccaatagcga ttctcgttga cgtagagatg cgacgcacga
     3901 tccgccaaac gacggagagc gtcgcggaac gttgcgacga tctcgccggg ctgcacacat
     3961 ccaaggatga tccgtttgtc ctcgatcccc ttcgtcgcag agcgaagtct tggagcggtt
     4021 cccatgaaga tcgtgcgtgc cacacggcgc gccgccgata aacggccgaa gtttggattc
     4081 tctttgtcga gaccgagggg aagagagtcg gtgccatcca cctcgcgctc gacaactggc
     4141 cgcagcgcgt cgtccaaata caacatcaac tcgttcaccg tcgcagagtc gtagagcgtc
     4201 acagatgcag gcatgatgag aaggctcttg tcctcatctc tccagagcgc atggatgacg
     4261 gcagccatca gacgaaggac tccgcgcgtg cgctggaatc gttggagcga tgaccaatcc
     4321 tcgtatagcc ggtcgaacag ttcgggatgc aacggatacg acgactccat ccggcgcctg
     4381 tagtcccctt cgctgcaacc gctcggaaac tccgacttct gcgtgttgta cgtgtcgccg
     4441 aacgcgcgaa tgacggcgtc gcgatgcttg tagacatccg ccgtcatcgg ttggaacagg
     4501 cgccgccgaa cgatctcgaa actctcctcc gcgctggctg gtcgccacgg cgactgcatc
     4561 cggccgaaga cattttcgag ccgcttgagc gccgtcctgc cggcctcgcc gccgacttcg
     4621 atgtcctcga cccagggact cgcccgttca tgctgatcat ccgaggccgg gacgctcgcg
     4681 accacgagtg ttttcttgca ggccctcgcg gcctcggtca gttcctgcgc aaagctgaag
     4741 tttgaatcga acgttccggc aggcagatca tccttgccga cgagctgtcg cgcgtgcgcg
     4801 acccattcgt cgatgaggac gaggcatggc tccgcgagcg tgaaaagctc tcgcagcgcg
     4861 gtggaaccgg gactgactcc tttgcggtca gcctcggcta cgagctcgta gccttccttt
     4921 ttcagaagct gccaggcaag ctcgccccac atcgtccgga cgaccgtgcc gtccttcttc
     4981 ttgatcggct ccgcgagcgg tagctcttgc ccgacgagca ccgctcgatt ggctttcggc
     5041 agcttttcga gctttgcttc agctaagact ggctcgatat cgcgcagctt gtccgccgag
     5101 actccgctga agaagtggta gagcgcgatc atcgagtgtg tcttgccgcc gccgaaattt
     5161 gtttgcagct cgacgatggg atcgccaccc attcccgaca gccgctgcaa cgcacccgag
     5221 agaagtgtgc gcaggccgag ggtgatgtag gtgcgctgga agaactggga gggatcgcga
     5281 tactcatcgc ttccttcgtt gcggtggacc tgccccaggt ctgctgcaaa ttcggcctgt
     5341 tgatagcggc ctgagtggac atcaggatgc ggcgtgatga cctcccgcca cggcttcagt
     5401 ccagagctcg gggacacgcc tgtctcgacc gcagcgacag ccgctttaac cgtctccttc
     5461 ttggcttgct cttcaaagcg aatgcgaagc agttcctgct tttgccgatc gagctcgatc
     5521 gcctctttcg cgccgatcgc ggtcagcagc cggtggacgt tgtcgaagac gcgataggtg
     5581 tcatcgctgg tgaagacttc ctgatgtgcc cagcgattgc ggatgtcacg catctcgccg
     5641 acgatcgaac gctcagtctg accaagcccg cgcttgaata cgttctgcca ctcgccgatc
     5701 atcacgctga gaagcgcagg tgtgtcccag ttccccgctt tcaaaagcgg ccggtcgccg
     5761 agggcgagaa tcgcctgctt ctcccacttc gctgcgccgt agaccgactt catctcccgc
     5821 tcgacgtagg gaagcagtcc tcggttgaga agctccaacg cctggtcgac tcgctgtctg
     5881 ttcgtgactg ccatgttcag ccttcctgag tcagccagcg aagaacttga tcccctggtt
     5941 gatgtcttcg tgttcgaggt ctggaggcct catcgttggc attattccag acaaagccgc
     6001 tgttctttgc cgcgcacctt gccgaattga attcgcggca ctacattgca attagctcat
     6061 ccaccacggt gtggaaggag taacggggtc gtcctaacgg gcggccccag cttttttagg
     6121 ctgagtctct ctcgtattcg cgtcgcaact tcgcccggcg atgctcctgc tcgacgcagt
     6181 aggccgtcca aagaaggaga tactttccgc ggtcggccag cactacgacg tagctgaaat
     6241 cctcgagcgc gatcacgatc cgttttttgt ctccaggcct tgacgagcgc caggagacga
     6301 cgccctttcc cggcgagtgc tcaaccatcg gccgcggcca gcgaatccgt tcgcagcggc
     6361 gcaggtcggg caggcggtcc tcctccactc ccccctcgct gatcaagtgc cagaacgttc
     6421 cctctttccc ctggatcaat gggtgccgct tgcatgcgca gcggcgcccg tcgtacgtgg
     6481 gcgacgaagc gatgaagtct gccttgaaga agccgtagac gacctcgacg tagcgctgcc
     6541 agtcgcctcc ggagtcggca agcacaacga gcggaggcag ccagtcgaga tccggcatgc
     6601 gattcaggct gcgttccaac gaaagatgtt gaccttcgtt gcgcggacga gcgaagagag
     6661 cgtaagccga taccctgagc gttgacgcat gcgctcgacg agcgtctgct tggccgcgct
     6721 tccggagatc cctcggttga catacgaaac gattccgatc agaaagtccg cgagctggat
     6781 ctgttcgact tcacacgaac gaacgctttg cagtctctca atcacttcca ggtcgcggtc
     6841 gtgaaggctg gtgcagagga tctccctcag tttctgctcc ttggcgtgac tcctcgtgtc
     6901 cttgatgtcg agatagatgt tgtaacgatc gtcgggattg aggatgtttt tcaagaggga
     6961 gtagtacatc ttgtaataga acgtgtcgtg atcctgcccg aagtcggcgt ggcgaagaga
     7021 gcccttatcc gggacgatca cggcgcggaa gcgaagatcg tcatcgtcga agaagtagtc
     7081 gagtagttcg agatagaacc ccagtttcga aggcgacacc ttcgtccact tgacctcgta
     7141 ccaccgcggc aggccatgct tttgtttgat ctcacgcata cgcacggcga tttcgcggac
     7201 cttctcctcc ggacaccaga ttgcgccgag aaccatgacc tttgaggtgt catgttccag
     7261 gtggcaactc tcgtcgcaat agatgttgaa gccggtcatg ctttgaacat cgtcccttgt
     7321 gcagacgcgg aggtgttgga cgagtcagcg gccagccgcg agatctccgg ccatgcgacg
     7381 acgagagagt tgtaggcgat tgcctcctcg gtccagcctt tgcgctcgca gacggaatag
     7441 aggcggtagg cgagatcgcg agccatctcg cccatgccac cgactttgcg gaggaggtca
     7501 gccgcgtcct gttcgccgcc gctcttgagg cgctggatca ggtgctgtgc cacttcccat
     7561 atggtgaggc gtttgtcgga taccggatcc cagtcgatat cgagttgttc gcgtccgaga
     7621 agctggactt tcccttttgc ggcccgaagg a
//
LOCUS       DATLVW010000091         7186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_129390, whole genome shotgun sequence.
ACCESSION   DATLVW010000091 DATLVW010000000
VERSION     DATLVW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129390"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VNN25_03085"
     CDS             <1..600
                     /locus_tag="VNN25_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90539.1"
                     /translation="GGGTAVVSWDADSLPAAAEEWEAFLSIDGGKRYAIRITPHLDIA
                     RHQASFPVPNITTGDARLLLRFGNERSETEVELPASFAIEGTFDGTPIWPSVSTSAAP
                     GEEARHGDGGVVAWVAGSRDGGGSHLVTASIPESMRGQSLCSGTGAPSQSAAMQRTPL
                     TAPPDQSPLSSSFTHQQLPSHSRKAAFTDVLLVSGRLNI"
     gene            752..2806
                     /locus_tag="VNN25_03090"
     CDS             752..2806
                     /locus_tag="VNN25_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH90540.1"
                     /translation="MKKILLILLLITSVAAAQTPVVPPQHETIEVTATRVAEDVLIVP
                     ASVTVIDGDDLRARNATDLGSALSTVAGVSIAPGGEGGPAGAVPEIWGLREFDAFLLV
                     VDGVPWGGAFNPDLPTLDLTDVDRIEVLRGSAPVMYGATSFVGVIHVIHRAAGAAGAG
                     HVSAGSYGSGSIAASVPISQTPELQQSISANADRRRFRDDDTGFDRIHALYRASSQHT
                     AGTFRFDADLTGIRQDPNSPHPRIGSSLTSDVPIDANHNPGDAHLDQNRIHLVAGWDA
                     KLAGSPWTTTIAVSHSTFDIVRGFLTDVVTTDPNAFGFSQNRNVNDVYFDTHIVRTIS
                     PELRFVAGLDHLYGNAHATSATFDYFAPLNGGRGERSGDVPVFEQFDARDRRNFSGLY
                     ATSQWTANPRLRFDVGARLNRTTESRHTDGPDGPESETRSFNKLSGSAGASYTLFTRG
                     QDTAMLFADYRNTFKPAAIDFGPEAEVDILNPETASSYEAGAKGRCQDGKLRWTVSLF
                     QMDFRNLVIATTVDGSPVLQNAGKERFQGAEAEVEYEWAPALRAEFGYSYHDARFRDF
                     IKEFDPGVPTQLAGKRLEMTPFNLASAGLLFAPKGGFNANASINYVGDRWLNMRNTAP
                     AKSYTTWSAGLGYRMTRGELRLDGHNLTNVRPPVAESELGDAQYYLLPARSFDVSYRW
                     FF"
     gene            complement(2852..3916)
                     /locus_tag="VNN25_03095"
     CDS             complement(2852..3916)
                     /locus_tag="VNN25_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXH90541.1"
                     /translation="MAIQKIDRFLKLMNDRGASDFHLTVGRPPMLRASGTMESIRYRT
                     LSQADFTELVKPIVPERMWEDYLLTGDADLAYEIPGQARYRVNLFRQQRGGGAVFRVI
                     PTKIMTIEQLGLPEQVRRVASIRSGLVLVTGPTGSGKSTTLAAIIDEINENRSLHIIT
                     IEDPIEFVHSNKKSLVHQREVGSHAKSFAEALKAAGREDPDVILVGEMRDLETIAMAL
                     SAAERGTLVFGTLHTNNAAKTMDRIISVFPAPEQEGVRNVLGETIRGVVAQQLMPRIG
                     GGRVAALEILFASPAIGNMIREGKTSQVTSAIQTGVREGMVDMDTSIRRLFKDGKVTA
                     RAAYDKAIDKEQFKDLLEKS"
     gene            complement(3916..6420)
                     /locus_tag="VNN25_03100"
     CDS             complement(3916..6420)
                     /locus_tag="VNN25_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXH90542.1"
                     /translation="MALFSSAVSEALKSLAAKTYATQEDRDVLLGRVEAADGVRARDV
                     MWMLFRPDRALRDSAVRVLMRIRDAETLDQFLAESKGKPDQALRAAAVSLFSLGLTGL
                     DARFAQLLAPPAKETKEWRELQEATRRLLAEAPVTRQIEPLLWQLATTGPADERMPFL
                     NKLSANEFDDNAIKRWRRFAAEPEHAVRDKAIEILATRAPQQSLDLLIEALPNSGYGL
                     QQIIVEAITRAAAGQGAAFADKILPGLASGDAATRTSILKILLGIGDPAGVIKRYIRF
                     SSSLAGFVRDRTLESLREFGSALVEPVIELLHDADPDTRSGAMAVAGVFEDVRIVPAT
                     IELLRDADWWIRISAADTLGRLKDPRAVEGLVSVLGDPDVKWAAVEALGRIADPRALP
                     ALGKMLADPSPDVRIEVMLALKNFKHPQVLNAVTSIAKSDPDRGVRTQAVEILDEMAS
                     LDRTSQEQVDAIRKTALAVSGSQGEPKLNTMLIATRNQGASDFHLAVGQPPIVRLSAD
                     LLRAQGEPFTSEQTASMLKEVLTEKQWTALETLHQVDFCYVVPQGGRYRANVFFDHRG
                     YNAVFRVIPDQPPTIVDLGLPLHLAEISDYHQGLVLICGPSGSGKSTTLAALVNLFNE
                     TRKDHVLTMEDPVEFVHPFKNCLVNQREVGTHTDSFARALRAALREDPDVIVIGELRD
                     NESISLALTAAETGHIVLGTLNSTSAPKAIDRILSSFPADEQPQIRASLSESLKYCIA
                     QRLLPGKEARKQVAAFEILKGTGNIGTMIRDEKTYQIYSAMQIGRSQGMQTFDEALKD
                     LLRRDQITAETAYMAALKKEDFESMVSAEFVARGKA"
     gene            complement(6553..7035)
                     /locus_tag="VNN25_03105"
     CDS             complement(6553..7035)
                     /locus_tag="VNN25_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90543.1"
                     /translation="MRHIFGAFLALVLVALPALAVDAGHAEGALVIDGVKYQLNFAYA
                     IAKQKNQLTDRNEMTRIILTDRALPDGTKLTDLDNTLPGDLNGVIICVDKQGRVGHVA
                     VQHPTGNYDGGYFEGIPDYEYKPHKGDSGTIGGSVSSLRVKTNTMSFSYDATFVAALK
                     "
BASE COUNT         1262 a         2277 c         2303 g         1344 t
ORIGIN      
        1 ggcggcggta cagccgtggt gtcgtgggat gcggattcgc ttccggcagc agccgaagag
       61 tgggaggcat ttctctcgat cgacggtgga aagcgttatg cgattcgcat cacgccgcat
      121 ctcgacatcg cccgccatca ggcctcattc cccgtcccca acatcaccac cggcgacgcg
      181 cgcctgctcc tccgcttcgg caatgagagg agcgagaccg aagtcgagct tccggcttcc
      241 tttgcgatcg agggaacgtt cgatgggacg ccgatctggc cctctgtctc gacatccgcg
      301 gcacctggag aagaagcgcg tcacggtgat ggtggcgtcg tcgcctgggt ggccggcagc
      361 cgcgatggcg gcggatcgca tctcgtcacg gcaagcatcc ccgaaagcat gcgcggccag
      421 agcctttgct ccggcaccgg cgcgccttcg caatctgccg caatgcagcg gacgccgctc
      481 acggcacctc ccgatcaatc gccgctctct tccagcttca cgcatcaaca gctgccttcc
      541 cattccagga aagcggcgtt caccgatgtc ctcctcgttt cgggccggtt gaacatttag
      601 gagcgatcct ccgctctcgc cgatgttccg cgaggccgga gccggctgat tctcgaatcc
      661 ctaccccgtc tgaacgctcg cccgacgtgc cgggttcgtg aaccacgcct gtctccggca
      721 gcgaacacct tttcgttgga ggactcgttc cgtgaaaaaa attctgttga ttcttctctt
      781 gattacgagc gtcgccgccg cgcagacgcc cgttgttccg ccgcagcacg aaacgatcga
      841 agtcacggct acgagagtgg ctgaagacgt cctcatcgtt cccgcctcgg tcaccgtcat
      901 cgacggcgac gatcttcgcg cacgcaacgc caccgacctc ggctccgccc tctccaccgt
      961 tgcgggcgtc tcgatcgcgc cgggcggcga aggcggccct gctggcgctg ttcctgagat
     1021 ctggggactt cgcgagttcg acgcctttct gctcgtcgtc gacggcgtac cgtggggcgg
     1081 cgctttcaat cccgatctgc ccacgctcga tctcaccgat gtcgaccgga tcgaagtgct
     1141 tcgcggatcg gcgcccgtga tgtacggcgc aacgtcgttc gtcggcgtga ttcacgtcat
     1201 ccatcgcgca gccggagccg ccggagccgg ccatgtctcc gccggctcat acggaagcgg
     1261 atcgatcgct gcgtcggtgc cgatctcgca gactcccgag ctgcagcaat cgatcagcgc
     1321 gaatgccgac cgcagacgtt tccgcgacga cgacaccgga ttcgaccgca tccacgcgct
     1381 ttatcgtgca tcgtcgcagc acacggccgg aacgttccgc ttcgatgcgg acctgaccgg
     1441 cattcgtcag gaccccaaca gccctcaccc gcgcatcgga agcagtctta ccagcgacgt
     1501 tccgatcgat gccaaccaca atcccggcga tgctcatctc gatcagaacc gcatccatct
     1561 cgtcgcaggc tgggacgcga aactggccgg ctcgccgtgg acgacgacga tcgccgtttc
     1621 tcattcaacc ttcgacatcg tgcgcggttt cctcaccgat gtcgtcacga ccgatccgaa
     1681 tgcgttcggc ttctcacaga accgcaacgt caacgacgtc tacttcgata cgcatatcgt
     1741 ccgcacgatc tcgccggagt tgcggttcgt cgccggactc gatcatcttt acggcaacgc
     1801 tcacgcgacg agcgcgacgt tcgactactt cgcgcctctg aacggcgggc gcggcgaacg
     1861 cagcggcgac gttcccgttt tcgaacagtt cgatgcgcgc gaccgccgga acttctccgg
     1921 tctctacgca acctcacaat ggaccgccaa cccgcgcctc cgcttcgacg ttggcgcgcg
     1981 cctgaaccgg acgacggaaa gccgccatac ggatgggccc gacggcccgg agtcggagac
     2041 gcgatcgttc aataagctct ccggaagcgc cggcgccagc tacaccctgt tcacgcgcgg
     2101 ccaggacacg gccatgctct tcgccgacta ccgcaataca ttcaaacctg cggcaatcga
     2161 cttcggcccc gaggcggagg tggacattct caatccggag acggcatcca gctacgaagc
     2221 gggcgcgaaa gggcgttgcc aggacgggaa gctgcgctgg acggtttcgc tgttccagat
     2281 ggacttccga aacctcgtga tcgctacgac ggtcgatggc tcgcctgtcc tccagaatgc
     2341 cggcaaagaa cgcttccagg gggcggaagc ggaggtggag tacgaatggg cgccggcact
     2401 tcgtgcggaa ttcggctaca gctaccacga cgcgcgtttc cgcgacttca tcaaggagtt
     2461 cgatccgggt gtccccacgc aactggcagg gaagcggctg gagatgacgc cgttcaatct
     2521 cgccagcgcc ggtctgcttt tcgcgcccaa gggcggattc aacgccaacg ccagcatcaa
     2581 ctacgtcggc gaccgttggc tgaacatgcg caacaccgcg ccggcaaagt cctacaccac
     2641 ctggtccgcc ggcctcggat accgaatgac ccgcggcgag ttgcgcctcg atggccacaa
     2701 cctgaccaac gtccgccctc cggtcgcaga gagcgagctt ggcgacgcgc agtactacct
     2761 tctgccagcg cgcagcttcg acgtgtccta ccgctggttc ttctgattcc ggtcgtcgca
     2821 ggcgggcggc tcgtccgcgc gcctgcgaga cctaggactt ctccagcaga tccttgaact
     2881 gctccttatc gatcgccttg tcgtaggccg cgcgggccgt cactttgcca tctttgaaga
     2941 ggcggcggat cgatgtgtcc atgtcgacca ttccttcgcg cacgccggtc tggatcgccg
     3001 acgtgacctg cgaggttttg ccttcgcgga tcatgttgcc gatggcgggc gaggcgaaga
     3061 ggatctcgag ggcggcgacg cggccgccgc cgattctggg catgagttgc tgggcgacga
     3121 cgccacggat cgtttcgccg agaacgttgc gaacgccttc ctgctcgggg gcggggaaga
     3181 cgctgatgat gcggtccatc gtcttggcgg cgttgttcgt gtggagcgtg ccgaagacga
     3241 gcgttcctcg ttccgccgcg gacaaggcca tggcgatcgt ttcgaggtcg cgcatctcgc
     3301 cgaccaggat cacgtccggg tcttcgcgcc ccgccgcctt cagcgcctcg gcgaacgatt
     3361 tcgcgtgcga gccgacctcg cgctgatgca cgaggctctt cttgttcgaa tgaacgaatt
     3421 cgatcgggtc ttcgatggtg atgatgtgca gactgcggtt ctcgttgatc tcgtcgatga
     3481 tcgcggccag cgtcgtcgac ttgccggagc cggtcgggcc ggtgacgagc accaggccgc
     3541 tgcggatcga tgcgacgcgg cgcacctgct cgggcagtcc gagctgctcg atggtcatga
     3601 tcttggttgg gatgacgcgg aagaccgcgc cgccgccgcg ctgctgccgg aagagattca
     3661 cgcgatagcg cgcctgacct ggaatctcgt aggcgaggtc ggcgtcgccg gtcagcaggt
     3721 aatcctccca catccgttcc ggcacaatcg gcttcaccaa ctccgtgaaa tcggcctggg
     3781 agagcgtgcg atagcgaatc gactccatcg ttcccgaggc gcgcaacatc ggcggacggc
     3841 cgacggtcag atgaaaatcc gacgcgccgc ggtcgttcat cagcttgagg aagcggtcga
     3901 tcttctgaat cgccattacg cttttcctcg ggccacgaac tcggcactga ccatcgattc
     3961 gaagtcttcc ttcttcagcg cggccatgta cgccgtctcc gcagtgatct ggtcgcggcg
     4021 gagaagatct ttcagagcct catcgaacgt ctgcattccc tgcgagcggc caatctgcat
     4081 cgctgagtag atctggtacg tcttttcgtc gcgaatcatc gttccgatgt tgcccgtccc
     4141 tttgaggatc tcgaaggcgg cgacctgttt tcgcgcttcc ttccccggca gcaggcgttg
     4201 cgcaatgcag tacttgagcg actcggagag cgatgcgcga atctgcggct gctcgtcggc
     4261 gggaaaactg gagaggatgc ggtcgatcgc tttgggtgcg ctcgtcgagt tgagggtgcc
     4321 gaggacgatg tggccggtct cggcggcagt gagtgcgagc gagatcgact cgttgtcgcg
     4381 cagctcgccg atcacgatca catcgggatc ttcgcgcaac gccgcgcgca acgcacgcgc
     4441 gaacgaatcg gtgtgcgtac cgacctcgcg ctggttgacg agacagttct tgaacggatg
     4501 cacgaactcg accggatcct ccatcgtcag gacgtgatcc ttgcgcgtct cgttgaagag
     4561 attgacgaga gcggccaggg tcgtcgactt tcccgacccg gacggaccgc agatgagcac
     4621 gaggccctgg tgatagtcgg agatctccgc gagatgcaac ggcagtccga gatcgacgat
     4681 ggtcggcggc tgatccggaa tcacccggaa gacggcgttg taaccgcgat gatcgaagaa
     4741 gacgttggcg cggtaacgtc caccctgcgg aacgacgtag cagaagtcca cctggtgcaa
     4801 ggtctccagc gccgtccact gcttttcggt cagcacttcc ttcagcatcg acgccgtctg
     4861 ctccgaggtg aagggctcgc cctgcgcgcg caacaggtcg gccgagagcc ggacgatcgg
     4921 aggctgcccg acagcgagat gaaagtcgga cgcgccctga ttgcgcgtgg cgatcaacat
     4981 ggtgttgagt ttcggctcgc cctgcgagcc ggagacggcc agggcagtct tccgaatggc
     5041 gtcgacctgt tcctgcgacg tacggtcgag cgatgccatc tcatcgagga tctcgactgc
     5101 ctgggtgcgc acgccgcggt cgggatcgct cttggcgata ctcgtgactg cgttgagcac
     5161 ctgcggatgc ttgaagttct tgagtgcgag catcacttcg atgcgcacgt ccggcgaggg
     5221 atcggccagc atcttgccga gggccggcaa tgcgcgcgga tcggcgatgc gtccgagcgc
     5281 ctcgaccgcc gcccacttca cgtcgggatc gccgagaaca ctgaccagac cttccacggc
     5341 gcgcggatct ttgaggcggc cgagggtgtc ggcggcggag atgcggatcc accagtccgc
     5401 atcgcgcagc aattcgatcg tggcaggcac gatgcggaca tcctcgaaaa cgccagcgac
     5461 tgccatggct ccggagcggg tatcgggatc ggcatcatgg agcagctcga tgacaggctc
     5521 gacaagcgca ctaccgaatt cgcgcagcga ctcgagcgtg cggtcgcgaa cgaagcccgc
     5581 cagactgctc gaaaagcgga tgtagcgctt gatcacgccc gcgggatcgc cgatgccgag
     5641 gaggattttc aggatggatg tgcgcgtcgc ggcatcgccg gaggcgagtc cgggaaggat
     5701 cttgtccgcg aatgcggcgc cctgccctgc cgccgcgcgc gtaatcgcct cgacgatgat
     5761 ctgttgtagt ccgtagccgg agttgggaag agcctcgatc agcaggtcga gcgattgctg
     5821 cggcgcgcgg gtcgcgagaa tctcgatggc tttgtcgcgg actgcatgtt cgggctcggc
     5881 agcgaaacgg cgccagcgct tgatggcgtt gtcatcgaat tcgttggccg agagtttgtt
     5941 gagaaacggc atccgctcgt cggcggggcc ggtggtagcg agttgccaga gcagcggttc
     6001 gatctgccgc gtgacgggcg cttcggcgag aagacggcgc gtcgcctcct gcagctcgcg
     6061 ccactctttc gtctccttcg ccggcggagc aaggagctgc gcgaaacggg catcgagtcc
     6121 ggtcagcccg agcgagaaaa gagataccgc ggccgcgcgc aacgcctgat cgggcttgcc
     6181 tttggactcg gcgaggaatt ggtcgagcgt ctccgcatcg cgaatgcgca tgagcacgcg
     6241 cacggccgag tcgcggagcg cgcggtcggg acggaacagc atccacatga cgtcgcgggc
     6301 gcgcacgccg tccgccgctt cgacgcggcc gagcagcacg tcgcggtctt cctgcgtcgc
     6361 gtaggtcttc gcagcgagac tcttcagcgc ctcgctgacg gcagaagaaa agagggccat
     6421 ggacgggcga agtgtacaac ggtggggacg ttagaaaacc tgcggcggta ttcacggtca
     6481 acagatgcag gctgcagccc gacagcactc aggcgagacg cctgagtgcc cgcggtcgag
     6541 acgaccgcac tcctacttca gcgcggccac gaacgtcgcg tcgtatgaaa acgacatcgt
     6601 gttcgtcttc acgcggagcg aggagacgga tccgccgatc gtgcccgagt cgcctttgtg
     6661 aggtttgtac tcgtagtcgg ggatcccttc gaagtagccg ccatcgtagt tgccggtcgg
     6721 atgctggacg gcaacgtgtc cgacgcgccc ctgtttatcg acgcagatga tcacgccgtt
     6781 cagatcgccg ggaagcgtgt tgtcgaggtc cgtgagcttg gtgccgtcgg gaagggcgcg
     6841 gtccgtgagg atgatgcggg tcatttcgtt gcggtcggtg agctggttct tctgcttcgc
     6901 gatcgcatac gcaaagttga gctgatactt cacaccatcg atgacgagag cgccttccgc
     6961 gtggccggca tcgaccgcga gggctggcag agcaacgaga acgagcgcaa gaaacgcccc
     7021 gaaaatgtga cgcatgacac acctccctgt ggcctgtgat cgggagtgta gcatcgggtg
     7081 cggggagcgt ggtgtaggta gtggggggtg gggaatgggg agtgggtggg gagttgggcg
     7141 tcggattttg ggcatcgcgg gctcgcaatc ccactcccca ctcccc
//
LOCUS       DATLVW010000092         8957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_3269, whole genome shotgun sequence.
ACCESSION   DATLVW010000092 DATLVW010000000
VERSION     DATLVW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3269"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VNN25_03110"
     CDS             <1..710
                     /locus_tag="VNN25_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH90544.1"
                     /translation="GPSAQALRDVSVGIAKTLNCLNETQGDDCTACQRIERRIHPDVH
                     FIEVAGERKMISVEQIREIVAAASLRPFEGRNKIFIIDPAHAISVGGSNSLLKTLEEP
                     ARDTTFILLTRSPDLLLPTIRSRCQMIFVGGEPEENDALAASIAAALTRFSDNRDSAA
                     LLGLASLIASQDEVSDAVALLGKVLCDAVAGRLPLSIEHERLLAAADATTAAIRWLNV
                     NADARMLVEQALAELVV"
     gene            1221..3119
                     /gene="selB"
                     /locus_tag="VNN25_03115"
     CDS             1221..3119
                     /gene="selB"
                     /locus_tag="VNN25_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HXH90545.1"
                     /translation="MSRKKRVVGTAGHIDHGKTALVLALTGTDTDRLPEEKRRGITID
                     LGFASWFTDDYQIGFIDVPGHERFVKNMLAGIGGIDSALLVVAADESIKPQTREHFAI
                     CRLLDIKTGVIAITKRDLVEDDILDLVRMEIEEMVAGSFLAGKPIVPVSSVTRAGLDD
                     LRHAIVASVAEVEDRDASTRVFRLPIDRVFTMKGFGSVITGTTFAGRLHVDSDVEVLP
                     GGKRSRARNIQVHGESRDSASAGERTSVNLPDIDLPELHRGQQLVTPGTLRPSQIITA
                     RLDLLPASFGGAKPLKDQTRIRFHHLSAELLGSIRFIDETADLKPGTGAYVQIRLESP
                     VVAVAGDRFVIRRYSPAITIGGGVILDTHLPKLSRNTRAGLLDVLATGTLQERVELMA
                     KLEGLRGLSMHEVQARTGIRVETLAKELKNVPHLADSGERRWIHQDVITDFRRRAMDF
                     LRNYFEENRMALNVPKSEFVQKLLPRGADAAMINFLLQDLAREKIVAIAGDALDIPGR
                     SKTLGGAEGELARMIEGRFAEAGLAPPPVSELINERTQKARVIEGVIGFLVKQGNLVR
                     LAEGIYVHRDALAAARERISTRSGDTIDVGQFKEFFGLSRKIAIPLLEWMDREGVTKR
                     MGDVRKVL"
     gene            complement(3179..3490)
                     /locus_tag="VNN25_03120"
     CDS             complement(3179..3490)
                     /locus_tag="VNN25_03120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HXH90546.1"
                     /translation="MKRGEVRWYRFQNPDKRRPVVVLTRDAMLDYLSEVTIVPITSTV
                     RDIPSEVILGPEDGMPRRCAINLDHVQTVSKGRLGGIVATLPSARMIEVRDALLFALG
                     Y"
     gene            complement(3487..3717)
                     /locus_tag="VNN25_03125"
     CDS             complement(3487..3717)
                     /locus_tag="VNN25_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXH90547.1"
                     /translation="MRTVQMTLDEELVTQVDRAARRLKTTRSAFTREALREALKRVTT
                     LQREAKHRRGYEEHPVKPDEFGDWEAEQVWPE"
     gene            complement(4332..5087)
                     /locus_tag="VNN25_03130"
     CDS             complement(4332..5087)
                     /locus_tag="VNN25_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90548.1"
                     /translation="MGLARRKWLAALIVFLAACNRPPTNQARPAASGPKIHAVIVTIQ
                     TTMPPSPKTWMHTIVIANDRARSSDEVDEWRLFDFKEKSVTFVDDLSKTYRKVSFADV
                     EAAHRASLSQPAPEGMPRAQFVLGNAQKTLQGVVAKQSTIRIGAYQRELWIASHPLIP
                     LGLFAIMQASEPVSSPLAGVMRQADEVLIEIKGFPMSDHAELPYEKRKLVVDNEVVKI
                     EKGEVAASWLKVDGTYREVKVRPDTAKSHPETK"
     gene            complement(5087..7741)
                     /gene="polA"
                     /locus_tag="VNN25_03135"
     CDS             complement(5087..7741)
                     /gene="polA"
                     /locus_tag="VNN25_03135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXH90549.1"
                     /translation="MPPKRPTLFLVDGSNNVYRSYFAIRNLTNSAGLATNAVYGFTQT
                     LKKLLKDFEPDCIAVAFDISGAKTRHEAFADYKKDRRPMPDDLAIQLPLVNEVLEGFG
                     IPVIGVQDWEADDLLGTLACVARDRGYDVVLATSDKDFFQLVGNGIKLFHTGREVLYD
                     ANGVAGAFGLPPEKVVDVMAIWGDAIDNIPGVPGIGEKGAKALITEFGSLEGVYENLD
                     KLKPAQRKKLEESRDKAFMSRELARIKCDLPVDDIDFEKLKRQEPDRAKLHDVFSRLE
                     FASLMQEFLPQAPAVEREYRIAANAAEVRDFIAGATEVAVWFESVTAEGHDDLLAASL
                     SAKQAESLIVPIASSAGGSPELREALQEMLRSDRLFVAHDIKLQLRRLAANHWPLPTR
                     IADTMIMSYVINPGLPSHALGNVARDRLKQDVESRKDVVDTAPLFALDHEIGSTALSS
                     YQQYLGEKSDVAMALKEMLAPELGREPALIDIYDRIEMPLVPVLQRMEARGIKIDTAL
                     LRESSNNMAQQLAKLETEIYAEAGIEFNINSPQQLGQILFEKLQYPVSKKTKGTKAFS
                     TSVDVLQELASHGFAVPRLILSHRELHKLKSTYIDALPQLVAADGRVHTSFNQAVAAT
                     GRLSSSDPNLQNIPIRTELGREIRKAFIAEEGNVLLAADYSQVELRILAHISQDPDLI
                     ETFQRGADIHRATASKMFNVPEDQLTHDQRRAAKTINFGVLYGMSAFRLSNELNIPTS
                     QAKDFIDAYFGRYPKIQEYLDRTLEEARRSGKVTTLFGRVRYIPEIHNKSFTVRGNAE
                     RMATNAPIQGTAADILKLAMIALDERLEKAAQMLLTVHDEIVIEVPETRAADVAGIVK
                     ETMENIFPLAVPLKVDAHYGRSWFDAKE"
     gene            complement(7754..8032)
                     /locus_tag="VNN25_03140"
     CDS             complement(7754..8032)
                     /locus_tag="VNN25_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90550.1"
                     /translation="MNVDYENLENDIANGSFRETLREELILGFRQLHQSGERLPLASY
                     LSAQIAEVVNRGAAEPIGSDLAFHLYQEILAAVEAARDQVMGEPIPPS"
     gene            complement(8029..>8957)
                     /locus_tag="VNN25_03145"
     CDS             complement(8029..>8957)
                     /locus_tag="VNN25_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90551.1"
                     /translation="LVDAGSSDLVRTMLDLYQSQVLAFYDPLTHTYFALDQMPAAAGG
                     LLDNEMMRESVVIHELTHALQDQRFRAGARDLALQKDTDGSPALHALMEGEATFVMLD
                     YVLDRAGQKMSDVMGSPEALELLTSSMNAGDASIDPGTPKYFAESLKFPYAEGLKLVI
                     EGYRRGGWKMIDSMDENPPRSTREVLHPSEYFTRLAGSAASPPAFDDKSSLPGALTVE
                     HLGEFHWRFLVGEAGAGWVDDRVTVTQNLHCDTTVLVDTQWQDVAHARAFRDAYDAFL
                     RKRGLDPQVAVDGSHVKAAYGADDKLMESFLR"
BASE COUNT         1706 a         2751 c         2758 g         1742 t
ORIGIN      
        1 acggaccgtc ggcacaggcg ctgcgggatg tctccgtcgg aatcgcgaag acgctcaact
       61 gtctgaacga gacgcagggc gacgattgca cggcctgcca gcggatcgag cggaggatcc
      121 atccggacgt tcacttcatc gaggtcgccg gcgagcggaa gatgatcagc gtcgagcaga
      181 tccgcgagat cgttgccgcc gcctcgctgc gtccgttcga gggccgcaac aagatcttca
      241 tcatcgatcc cgcgcacgcc atcagtgtag gcggctcgaa ctcgctcctc aagacgctcg
      301 aagagccggc ccgcgatacg accttcatcc tcctgacgcg atcgccggat ctcctgctgc
      361 cgacgatccg ctcgcgctgc cagatgatct tcgtcggcgg cgagcccgaa gagaacgacg
      421 ccctggccgc gtcgatcgcc gcagccctga cgcgcttttc cgacaatcgc gacagcgcgg
      481 ctctcctcgg cctggcctcg ctcatcgcat cacaggacga ggtcagcgat gccgtggcat
      541 tgctcggaaa ggttctttgc gacgccgtag caggccggct gccgctctcg atcgagcacg
      601 agcggctcct cgccgccgcg gacgccacaa ccgccgcaat ccgctggctc aacgtgaacg
      661 ccgacgcgag gatgctggtc gagcaggcgt tggctgagct ggtggtttga ggttgattgt
      721 tggggtactg agctgttgag ctccccggaa ggtcagcaac ctggctcagc gaccaggcaa
      781 ccctccacgt aaccgtttac aaaaagaacc ggccccgctt tttagacggg gccggggaag
      841 atctactgga tgtgagtggg tcgaacgctc aggggagaga tgttgctgga tcggtaagat
      901 tcaaagaaga attcaagcgg taaatcgaga ggcgggcggt tgaaacggaa agctaatccg
      961 ccccacatca tccagtaaac ttgtcaaagt tcgaacggcg aaactgatta tcgcacgacc
     1021 ttgtgaaaaa tttctcaatg taacagcaac gtgacaaacc tcttagttgc tgagacataa
     1081 ccaacacttt cggaacggca attattgtag tacagaacga ccgttcggtc aatcatacgt
     1141 ggccgggccc ttccaggttt cgggcttcgt cactcgcaca cgccgttcgc gtgccagcat
     1201 tgctcggata cactcggctc atgtcccgca agaaacgtgt cgtcggcacg gccggccaca
     1261 tcgatcacgg caagacggct ctcgtgcttg ctctgaccgg taccgacacc gaccgccttc
     1321 ccgaagagaa gcgccgcggc atcaccatcg acctcggatt tgcgagctgg ttcaccgacg
     1381 actaccagat cggattcatc gacgttcccg gtcacgagcg cttcgtcaag aacatgctcg
     1441 cgggcatcgg cggcatcgac agcgcgctgc tcgtcgtcgc ggcggacgaa tcgatcaagc
     1501 cgcagacacg cgagcacttc gccatctgcc ggctgctcga catcaagacc ggcgtcattg
     1561 cgatcacgaa gcgcgatttg gtcgaggacg acatcctcga tctcgtgcga atggagatcg
     1621 aagagatggt ggccggaagc ttcctcgccg ggaagccgat cgtcccggtg agcagcgtga
     1681 cccgcgcagg tctcgacgat ctgcggcacg cgatcgttgc ttcggtcgcc gaggttgagg
     1741 accgcgatgc atcgacaagg gtgtttcggt tgccgatcga tcgcgtgttc acgatgaaag
     1801 gcttcggctc ggtgatcacc ggcacgacgt tcgccgggcg ccttcatgtc gacagcgacg
     1861 tcgaagtcct gccgggaggc aaacgcagcc gcgcccgcaa cattcaggta cacggcgaat
     1921 cgcgcgattc agcttcggcc ggagagcgga catccgtcaa cctgcccgat atcgatttgc
     1981 cggagcttca ccgcggccag cagttggtca cgccgggcac gctccgtccc tcgcagatca
     2041 ttaccgcgcg cctcgatctc ctgccggcct cgttcggcgg cgcaaagccg ctcaaagacc
     2101 agacccgcat ccgcttccat cacctttctg cggagctcct tggcagcatt cgcttcatcg
     2161 atgagacggc cgatctcaag ccgggaacgg gcgcctacgt ccagatccgt ctcgaatcgc
     2221 cagtcgtcgc cgtggccgga gaccggttcg tcatccgccg ctattcacca gcgattacga
     2281 tcggcggcgg agtgattctc gacacgcact tgcccaagct gagccgcaac acgcgcgccg
     2341 gtctgctcga cgttctcgcg accggcacgc tgcaggagcg cgtcgagctg atggcgaaac
     2401 tcgaaggact gcgcggcctt tcgatgcatg aggtgcaggc tcgcaccgga attcgcgttg
     2461 agacgctggc aaaggagctg aagaacgttc cgcatctcgc cgacagcggg gaacggcgct
     2521 ggattcatca ggacgtcatc accgactttc ggcgccgggc gatggacttt cttcgcaatt
     2581 acttcgaaga gaatcggatg gcgctcaacg tgcccaagag cgagttcgtg cagaagctcc
     2641 tgccgcgcgg cgcggatgcc gcgatgatca acttcctgct gcaggatctg gcgcgggaaa
     2701 aaatcgtagc gattgcgggc gacgccctcg acatccccgg ccgctccaag accctgggtg
     2761 gcgcggaagg ggagctggcg aggatgatcg agggacgatt cgccgaagcc ggtctggcac
     2821 ccccaccggt gtcggagctg atcaacgagc ggacgcagaa ggcgcgcgtg atcgaagggg
     2881 tgatcggatt cctggtcaag cagggcaacc tcgtccgcct cgcggagggg atctacgtgc
     2941 atcgcgacgc gttagccgcc gcgcgcgagc gcatcagcac acgttccggc gacacgatcg
     3001 acgtcggtca gttcaaagag ttcttcggcc tctcgcgcaa gatcgccatt ccgctgttgg
     3061 agtggatgga tcgcgagggc gtcacgaaga ggatggggga tgtgcggaag gtgttgtgaa
     3121 tggctcccgc agcgaagccg gctttcaacc aaaagtcggc acgttcagtt tttcgacgtc
     3181 agtacccgag cgcgaacaga agtgcgtcac gaacttcgat catgcgagcc gacggcaagg
     3241 tggcaacgat cccacccagt cggcccttcg aaactgtctg gacatgatcg aggttgatcg
     3301 cacaacggcg cggcatgccg tcttccggtc cgaggatgac ctccgatggg atgtcacgaa
     3361 cggtgctcgt gattggaacg atcgtcactt cgcttagata gtccagcatc gcatctcgcg
     3421 tgaggacgac gacgggacgg cgcttatccg gattttgaaa acgataccag cggacttcac
     3481 cccgcttcat tccggccaca cctgctcggc ttcccagtcg ccgaactcgt ctggtttcac
     3541 cgggtgttcc tcgtagccgc gtcgatgctt tgcttcgcgc tgcagtgtcg taacgcgctt
     3601 gagagcctcg cgaagcgctt cacgcgtgaa tgccgagcga gtcgtcttca accggcgggc
     3661 agcgcgatcc acctgcgtca ccagctcctc gtccagtgtc atttgcacgg ttctcatgtc
     3721 ccgattgtat cgctactcgg atgtggacga gtatagacac gacgcagaca tccaacgtga
     3781 gtcaacaact ggccagttta caatctctta gccatgagcg agacagacct tcccccagtt
     3841 tccgatgagc tggtgcgaga gctcaaggag cggctcagag ccacaccatg agtcgcccga
     3901 aagcagcgag ccatgggaat cagtggaccg cgaggtcttc ggcttggatt gaaacggtat
     3961 gcggcgggcc gctatcggcg accacgatca tcgaccaggc agaattccta gctggcgatg
     4021 cgccgatata tcttcttcga gatccgctgc cgagtaacga agcgggacct gatgctcctt
     4081 gagcagggcg tgcacctgga agcgggtttc gaatccaagc aagcggcgaa tctggctttc
     4141 ggtgagcgcg ccgtgcgata ggcctcaacg gcgatggctt ccagcgaacg ccgagggacg
     4201 cccaccgccc tccgagagca gccgaaatat cctcgggaag gtcgatcgtg actgtcatcg
     4261 tgttctcaat cttacgctta cgaaacggag tgtgtgcttc gcaaaagtcc gcatcaaaga
     4321 tgcgttaaca cctacttcgt ctccggatgc gactttgccg tgtccggacg caccttcact
     4381 tcccgatacg tgccatccac cttcagccac gacgccgcaa cctcgccctt ctcgatcttc
     4441 accacttcgt tgtcgacgac gagtttcctc ttctcgtacg gcaactcggc gtggtcggac
     4501 atggggaaac ctttgatctc gatcagcacc tcgtccgctt ggcgcatcac gccggcgagc
     4561 ggcgatgaca ccggctcgct ggcttgcatg atggcgaaga gacctagcgg aatgagtgga
     4621 tgcgatgcga tccagagctc gcgttggtag gcgccgatgc ggatcgtcga ctgctttgcc
     4681 accacaccct gaagcgtctt ctgcgcattt ccgaggacga attgcgcgcg cggcattcct
     4741 tccggtgccg gctgcgaaag ggacgcgcga tgcgcggctt cgacatccgc gaaggaaacc
     4801 ttccgatagg tcttggacag gtcatcgacg aacgtcacgc tcttttcttt gaaatcgaag
     4861 agtcgccatt cgtcgacttc gtcgctgctt cgggcgcgat cgttggcgat gacgatcgtg
     4921 tgcatccacg ttttcggcga cggcggcatc gtcgtctgga tcgtgacgat gaccgcgtgg
     4981 atcttcggac cgcttgcggc cggccgagcc tggttcgtcg gcgggcgatt acaagcggcc
     5041 aggaaaacga tcagcgcggc gagccatttc ctccgagcga gccccattac tccttcgcgt
     5101 cgaaccagct ccgcccgtag tgcgcgtcga ctttgagcgg caccgcgagc gggaagatgt
     5161 tctccatcgt ctctttcacg atccccgcca cgtcggcggc gcgcgtctcc ggcacttcga
     5221 tcacgatctc gtcgtgaacc gtcaaaagca tttgcgccgc cttctccagc cgctcgtcca
     5281 acgcgatcat cgccaacttc aggatgtccg cggccgttcc ctgaatcggc gcgttcgtgg
     5341 ccatccgttc agcgttgccg cgcaccgtga atgacttgtt gtggatctcg gggatgtagc
     5401 ggacacgccc aaacagcgta gtcactttgc cgctgcgccg cgcctcctcg agcgtccggt
     5461 cgaggtactc ctgaatcttc ggataacggc cgaagtaggc gtcgatgaag tccttcgcct
     5521 ggctggtcgg gatgttgagc tcattcgaca gacggaaggc ggacatgccg taaagcacgc
     5581 cgaagttgat cgtcttcgcg gcgcggcgct gatcgtgcgt aagctgatcc tccggcacgt
     5641 tgaacatctt cgacgcggtg gcgcgatgga tgtctgcgcc gcgttgaaac gtctcgatca
     5701 gatcggggtc ctgcgagatg tgcgcgagga tgcgcagctc gacctgcgag tagtcggccg
     5761 cgagcaacac gttcccttcc tcggcgatga acgcttttcg gatctcgcgt cccagctcgg
     5821 tgcggatcgg aatgttctga aggttcggat ccgacgatga cagtcgcccg gtcgcggcaa
     5881 cggcttggtt gaacgacgtg tgcacgcggc cgtccgcggc aacgagttgc ggaagcgcat
     5941 cgatgtaagt cgacttgagc ttgtgcagtt cgcgatgcga caggatcagg cgcggcaccg
     6001 cgaagccgtg actcgccaac tcctgcagga cgtcgaccga ggtcgagaat gccttcgttc
     6061 ccttcgtctt cttcgagacc gggtactgca gcttctcgaa cagaatctga ccaagttgtt
     6121 gcggcgagtt gatgttgaac tcgatccccg cttcggcgta gatctccgtc tcgagttttg
     6181 cgagctgctg cgccatgttg ttggacgact cccgcagcag agcggtgtcg atcttgatcc
     6241 cgcgcgcttc catccgctgc agcaccggaa cgagcggcat ttcgatgcgg tcgtagatat
     6301 cgatgagggc gggctcgcgg cccagttcgg gagcaagcat ctctttcagc gccatcgcca
     6361 catcggactt ttcgccgagg tattgctggt aggaagaaag ggcagtggaa ccgatctcgt
     6421 gatcgagcgc gaagagcggc gcggtatcga caacatcttt gcgcgactcg acgtcctgtt
     6481 tcagccggtc gcgcgcgacg ttgccgaggg cgtggctcgg gaggccggga ttgatgacgt
     6541 acgacatgat catcgtgtcg gcgatgcgtg tgggtagcgg ccagtggttc gcggccagac
     6601 ggcgcaactg cagcttgatg tcgtgcgcga cgaagagacg gtcggagcga agcatttcct
     6661 gcaaggcctc gcgaagctct ggactcccgc cggccgagct tgcgattggg acgatcagcg
     6721 attccgcctg cttcgccgac agcgacgccg ccagcagatc atcgtgaccc tccgccgtga
     6781 ccgactcgaa ccagaccgct acttcggtgg cgcccgcgat gaaatcgcgc acctcggcgg
     6841 cgttcgctgc gatgcggtac tcgcgctcaa ctgctggcgc ctgcggcaga aactcctgca
     6901 tcagcgaggc gaactcgaga cgcgagaaga cgtcgtgcag ctttgcacgg tcaggctcct
     6961 gccgtttgag cttctcgaag tcgatgtcgt cgacgggcag gtcgcacttg atgcgggcga
     7021 gctcgcgcga catgaacgct ttgtcgcggc tctcttcgag cttcttgcgt tgcgccggtt
     7081 ttagcttgtc gagattctcg taaacgcctt cgagcgagcc gaactccgtg atcagcgctt
     7141 tcgcaccctt ctcgccgatg cctggaacgc cggggatgtt atcgatggcg tcgccccaga
     7201 tggccatgac gtcgacgacc ttctcgggcg gcagtccaaa tgcgccagcc actccattcg
     7261 cgtcataaag gacttcgcgc ccggtgtgga acagcttgat gccgttgccg acgagctgga
     7321 agaagtcttt gtccgaggtg gcaagaacga cgtcgtaccc gcggtcgcgt gcgacgcagg
     7381 caagcgtgcc gagaagatcg tccgcctccc agtcctggac accgatcacg ggaatcccga
     7441 agccctcgag cacttcgttg acgagcggca actggatggc cagatcgtcg ggcatcggcc
     7501 ggcggtcttt cttgtagtcg gcaaaggcct cgtggcgtgt tttcgcgccg ctgatgtcga
     7561 aggcaacggc gatgcaatca ggctcgaagt ccttcaacag ctttttgagc gtctgcgtga
     7621 aaccgtaaac cgcgttcgtg gccagacccg ccgagttcgt caggttgcgg atcgcaaagt
     7681 aggagcggta gacgttgttg gaaccgtcga cgaggaagag ggttgggcgt ttcggcggca
     7741 tcagagcgcg attctatgac ggcgggatcg gctcccccat cacctggtcg cgggcggctt
     7801 caacggcggc cagaatctcc tggtagagat gaaaggccag atcgctgccg atcggttccg
     7861 ccgcaccgcg attcaccact tcggcgatct gcgcggagag gtacgacgcc aacggcaagc
     7921 gctcgcctga ctgatgaagc tgccggaagc ccaagatcag ctcttcccgc agcgtctcgc
     7981 ggaacgatcc gttggcaatg tcgttctcga ggttttcgta gtcgacattc atcgtaggaa
     8041 cgactccatc aacttgtcgt cggctccgta cgcggctttc acgtgcgagc cgtcgaccgc
     8101 cacttgagga tcgagtcccc gtttgcgcag gaaagcgtcg taggcgtcgc ggaaggctcg
     8161 cgcgtgcgca acatcctgcc attgcgtatc gacgaggacc gtcgtgtcgc aatgcagatt
     8221 ctgggtgacg gtgacccgat catcgaccca tccggcgccg gcctcaccga cgaggaagcg
     8281 ccagtgaaat tcgcctagat gttcgacggt cagagcgccg ggaagcgagg acttgtcatc
     8341 gaaagcaggc ggcgaggcgg cagacccggc cagacgcgtg aagtactccg aggggtgcag
     8401 cacctcgcga gtagaccgcg gcggattctc atccatgctg tcgatcatct tccagccgcc
     8461 gcgccggtag ccttcgatca cgagcttcag cccttccgca tagggaaact tcagcgactc
     8521 ggcaaaatac tttggcgtgc cgggatcgat cgaggcatcc ccggcattca ttgacgacgt
     8581 gagcagctcc aacgcctcgg gactgcccat cacgtcactc attttctgcc cggcgcgatc
     8641 gagcacatag tcgagcatga cgaacgtggc ctcgccttcc atcagcgcgt gcagtgccgg
     8701 gctgccgtcc gtgtccttct gcagcgcaag gtcgcgcgcg ccggcgcgga agcgctgatc
     8761 ctggagggcg tgcgtcaact catgaatcac gaccgactca cgcatcattt cgttgtccag
     8821 gagcccaccg gcggccgccg gcatctgatc gagcgcgaaa taggtgtgag tcagtggatc
     8881 gtagaaagcc agcacctgtg actgatagag atcgagcatc gtgcgtacga ggtcggacga
     8941 gccggcgtcg acgagtt
//
LOCUS       DATLVW010000093         5965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_375450, whole genome shotgun sequence.
ACCESSION   DATLVW010000093 DATLVW010000000
VERSION     DATLVW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375450"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VNN25_03150"
     CDS             <1..299
                     /locus_tag="VNN25_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90552.1"
                     /translation="KHPVVLDNFGTMPDARTFERAHEALLSRAETSLARYCDTNGIRY
                     LILDNPKFGVASANAITGKTQELQSTWWWRVHHGAEAQRFHLIWSEPGLRIYRR"
     gene            326..1798
                     /locus_tag="VNN25_03155"
     CDS             326..1798
                     /locus_tag="VNN25_03155"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HXH90553.1"
                     /translation="MKPPILERWFAPSVSPSFVRARWIWLRCLGAIFFSAFYSLYFQI
                     HGLIGPRGILPASDYLGAVHRALGLKGYWFAPTLLWFSAGDGMLSAIVWAGLAASVAI
                     ILNLWPRLSIAVAGICFLSFIGAAQDFAGYQSDGMLLEAAFLSIFLAPRGIRPRMGID
                     QPPSRAAVFLLQWEWFRIYFESGLVKLLSGDEQWRNLTAMDKYYENGPLPSWIGWYVQ
                     QWPHSFHAFSAGLTLFVELFVVWLLFAPRKGSLCRSIAFLIVTPLQISIILTANYAFL
                     NYLVLCAGVLLLDDSILRGPAPVPQKNKPSLVAAFVLTTIFLTTIASFMIPSFPTVTL
                     LDPFRIANRYGLFAVMTRQRIEIEFQGTSDGVNWVAYPFRYKPQDVRARPGIYAPYQP
                     RFEWNLWFASLGSVQDNQWVLNTEVRLMENSEPVLQLFAKNPFANKPPIAVRAVAWQY
                     WFTTREERARTGAWWRRELLGAYAPTARRTADGNVSFDSE"
     gene            complement(1833..2582)
                     /locus_tag="VNN25_03160"
     CDS             complement(1833..2582)
                     /locus_tag="VNN25_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH90554.1"
                     /translation="MDVGDASLSGIAAAIAEPARARMLCCLMDGHARTSTELATVAEV
                     SPSTASVHLARLKEQHLVNVLAQGKHRYYSLAGASVAAALEALMVVAGGSRDRFVPNT
                     PPRLRAARTCYDHLAGAVAVLLHDRFKERGWLEDSPAGDGDYDLSEVGAKAIESLGVD
                     VNAVRAMRRRFACSCVDWSERRPHLAGALGAALLNLALKRKWVIRDLDSRALSVTRIG
                     VREMGTRFGVQLNSTDHHRGTESTEIITATR"
     gene            2634..3074
                     /locus_tag="VNN25_03165"
     CDS             2634..3074
                     /locus_tag="VNN25_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH90555.1"
                     /translation="MTITCLRPSTPNRREALQLIAATGAALTLGCCVLTPLDSRRTLM
                     KTTAQDAATVTPKVVKVKLENEHVRVLDFMSEPGDKEDWHFHPAFVVYVVTGGTLRIT
                     TPDGKSEDVVFKAGDILYREPRTHRTENIGKTTLHAIIVELKSP"
     gene            3172..3327
                     /locus_tag="VNN25_03170"
     CDS             3172..3327
                     /locus_tag="VNN25_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXH90556.1"
                     /translation="MGNLLWLIIVILVILWLGGFAMHIGGGLIHLLLVIAVIVLIIQL
                     ITGRRVV"
     gene            complement(3368..4177)
                     /locus_tag="VNN25_03175"
     CDS             complement(3368..4177)
                     /locus_tag="VNN25_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HXH90557.1"
                     /translation="MLSRDDFAKRFSRNAIIGMVHVGALPGAPRFGGSMDAVIDAAMR
                     DARALCEGGCDAIAFENFGDRPFFKGRVPAETIAGLTRVITEVIAEVKLPFGVNVLRN
                     DALSAIAIAAATGAAFIRVNIHTGTMLTDQGIIEGRAAESLRLRAAIAPELLLFADHM
                     VKHAVPLAAVDEVQAAKDLRHRGLADGIIISGSETGAEADRGSFARVRDALPDAPILI
                     GSGLTEQNASAFGTADAAIAGTSIKIDGRLEAPVDSDRVARLVAAFKRSVS"
     gene            4316..5725
                     /gene="cysS"
                     /locus_tag="VNN25_03180"
     CDS             4316..5725
                     /gene="cysS"
                     /locus_tag="VNN25_03180"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXH90558.1"
                     /translation="MSDLKLFNTLTREKETFEPLVPGEVRMYSCGPTVYNHLHIGNMR
                     TFLWGDLLRRYLEFRGLRVKQIMNITDVEDKIIRNAAAAGKDLTTYTAPYIESFHDSL
                     AKLRVRRADAYPRATEYIPQMVALVQQLTERGHTYVADGSTYFKVSSLPGYGKLSRVE
                     IDQSSEYSRIESDEYEKESARDFVLWKSKKDNEPAWQTPIGEGRPGWHLECSAMSMDL
                     LGETFDIHTGAVDLIFPHHENEIAQSEGATGKPFVRYWIHGEHLNIDQQKMSKSLGNI
                     YTVPEVEEMGYDLLALRYALLSVPYRTKLNFTMQSLDDAKNALSRIESFLLRVDEVSR
                     SAAHDARHSDGAADQLIGKFLIDFETAMDDDLNTAGALGALFTLIREANTAIDEGRIL
                     AGDAEGLKTAVMKIDPVFDLFPKQRENLDAEIESLIAARNAARKARNFAESDRIRDEL
                     LGRGILLEDTPAGTRWRKG"
BASE COUNT         1131 a         1944 c         1756 g         1134 t
ORIGIN      
        1 gaaaacatcc cgtcgtcctc gacaacttcg gaaccatgcc cgatgcacgc acgttcgagc
       61 gcgcgcacga agcgctactc agccgcgcgg aaacgtcgct ggcccgctat tgcgacacca
      121 acggcatccg ctatctgatc ctcgacaatc cgaagttcgg cgtcgcctcg gcgaatgcga
      181 tcaccggcaa gactcaggag ttgcaatcaa cctggtggtg gcgcgttcac catggcgcgg
      241 aggcgcagcg ttttcactta atctggagcg agcctggctt gcgcatctac cgacgatgat
      301 gttcgaagaa tacacatcgg agccgatgaa gccgccgatc ctggagcggt ggtttgcgcc
      361 ctccgtctcg ccgtcattcg ttcgcgcccg ctggatctgg ctccgctgtc tcggcgcgat
      421 cttcttctcc gcgttctact cgctctactt ccagattcac ggcctgatcg gaccgcgcgg
      481 catcctcccc gcgagcgatt acctcggcgc cgtgcatcgc gcgctcggcc tcaaaggcta
      541 ctggttcgcg ccgacgctgc tctggttcag cgcgggcgac gggatgctga gcgcgatcgt
      601 ctgggctggc ctcgccgcct ccgtcgccat catcctcaat ctctggccgc ggctatcgat
      661 cgctgtggcc ggcatctgct tcctctcgtt catcggggcc gcgcaagact ttgccggata
      721 ccagtcggat ggaatgctgc tcgaagccgc gtttctctcg atcttcctcg cgccgcgcgg
      781 aatccggccg cgcatgggga tcgatcagcc gccgtcacgc gccgcggtct tcctgctgca
      841 gtgggagtgg ttccgcatct acttcgaatc agggctggtc aagctcctca gcggcgacga
      901 gcagtggcgc aacctgacgg caatggacaa gtactacgaa aacggtccgc tgccatcgtg
      961 gatcggctgg tacgtccagc agtggccgca ctcgttccat gccttctcgg ccgggctcac
     1021 gctcttcgtc gagttgttcg tagtctggct cttgttcgcg ccgcgcaagg gctcgctctg
     1081 ccgcagcatc gcctttctca tcgtcacccc gctgcagatc agcatcatcc tcactgccaa
     1141 ctacgcgttc ctcaactatc tcgtcctctg cgccggcgtg ctgctgctcg atgactcgat
     1201 ccttcgcgga cctgcacctg tgccgcagaa aaacaagcct tcgctcgtcg cggcattcgt
     1261 gttgacgacg attttcctca cgacgatcgc cagcttcatg attccgtcct ttccaaccgt
     1321 cacgctgctc gatcctttcc gcatcgccaa tcgctacggc ctcttcgcag tcatgacgcg
     1381 gcaacgcatc gagatcgagt tccagggaac cagcgacggc gtgaactggg tcgcctaccc
     1441 attccgctat aagccgcagg atgtgcgcgc gcggcccgga atctacgcgc cctaccaacc
     1501 gcgcttcgag tggaatctct ggttcgcgtc cctcgggtcg gtgcaggaca atcaatgggt
     1561 cctcaacacc gaagtgagac tgatggagaa ttcggagcct gtgctgcagt tgtttgcaaa
     1621 gaatccattc gcaaacaaac cgccgatcgc cgttcgcgcc gtggcgtggc aatactggtt
     1681 cacgacgcgc gaagaacggg cacgtaccgg agcgtggtgg cggcgcgagt tgctcggcgc
     1741 gtatgcgccg acggcgcggc gaacggcgga cggaaatgtt tcgttcgact ccgagtaact
     1801 tgtcaaaacc gtgaaccaca gagacacaga gatcaccgag ttgcagtgat gatctctgtg
     1861 ctctctgtgc ctcggtggtg atcagtactg ttcagctgaa cgccgaatcg cgtgcccatt
     1921 tcgcgaacgc ctatccgcgt aacgctcagg gcgcggctgt cgagatcccg gatgacccac
     1981 ttgcgcttca acgccaggtt cagaagggcg gcgccgaggg caccggcgag gtgggggcgg
     2041 cgctcgctcc agtcgacgca cgaacaggcg aaacggcggc gcatcgcgcg gacagcgttg
     2101 acgtcgacgc cgagggactc gatcgccttc gctccgacct cggagagatc gtagtcgcca
     2161 tcacccgctg gcgagtcttc cagccaccct cgctctttga agcgatcgtg gagtaagacg
     2221 gcgaccgcgc ctgccaggtg atcgtagcaa gtgcgtgccg cgcgcagacg aggcggggta
     2281 ttcggcacaa agcggtcgcg cgatccaccc gcgacgacca tcaacgcttc cagcgcggcg
     2341 gcgacgctgg ctcccgccag actgtagtaa cgatgtttgc cttgcgcgag cacgttcacg
     2401 aggtgctgct ccttgagtcg agccaggtgg acgcttgctg tcgatgggct cacctccgcc
     2461 acggtcgcaa gctccgtgct cgtccgcgcg tgaccatcca tcaggcagca gagcatgcgg
     2521 gcacgcgctg gttcagcgat ggcagcggcg attccagaga gtgaggcgtc acctacatcc
     2581 atactttgat gataaacgaa gcgtgcctgt tcagcaacgg cgatactgcg cccatgacga
     2641 ttacctgctt gcggccatcc acgccaaacc gccgcgaggc cttgcagctg atcgctgcga
     2701 cgggcgctgc cctgaccctc ggttgttgtg tactgacccc actggattca aggaggacat
     2761 tgatgaaaac gactgcgcag gacgcagcta ccgtgacgcc gaaggtggtt aaggtgaagc
     2821 ttgagaatga gcatgtgcgg gttctggact tcatgagcga gccaggcgac aaggaggatt
     2881 ggcacttcca ccccgccttc gtcgtgtacg tcgtgacggg gggcacgctg cgcattacca
     2941 cccctgacgg caagagcgag gatgtcgtat tcaaagccgg cgacatcctc tatcgggaac
     3001 ccagaaccca ccgcaccgaa aacatcggca agaccaccct ccacgccatc attgtggagc
     3061 tcaagagtcc gtaaggagtg ctggccatcc cggaggatcg aaccatcgat tctggcgtcc
     3121 ataaacgccg gcgtcaccgc gctggcacgg tagtggctat tgctgagccg catgggaaac
     3181 ttactttggc tcatcatcgt gatcctcgtc atcctctggc tcggtggctt tgcgatgcac
     3241 atcggcggtg gtctcatcca tctgctgctc gtcatcgctg tgattgtgct gatcattcag
     3301 ctcatcacgg gccggcgcgt cgtctagacc agccggcgtg cagcgtggcg accgcgccgc
     3361 acaccggcta gctgacggag cgtttgaacg cggccacaag ccgcgccaca cgatcggaat
     3421 cgacgggcgc ttcgaggcgt ccgtcgattt tgatcgaggt gccggcgatc gcggcatccg
     3481 cagtgccaaa cgctgacgcg ttctgctctg tcagcccgct gccgatgaga atcggcgcgt
     3541 ccggcagcgc gtcacgcacg cgtgcgaagc tgccgcgatc cgcttcagcg ccggtctccg
     3601 acccgctgat gatgatgccg tccgcgaggc cgcggtgccg caggtctttg gccgcttgga
     3661 cctcgtcgac tgctgcgagc gggacggcgt gtttgaccat gtgatcggcg aagagcagca
     3721 actccggcgc aatcgccgca cgcaatcgca gcgactcggc ggcacgtcct tcgatgatcc
     3781 cctgatcggt cagcatcgtt cccgtatgga tgttgacgcg aatgaacgcc gcgccggtag
     3841 cagccgcaat ggcgatcgcc gacaacgcat cgttccgaag cacgttcacg ccgaacggga
     3901 gtttcacctc cgcaatcact tcggtgatga cgcgcgtcag tcccgcgatg gtctccgcag
     3961 gcacgcgccc tttgaagaac ggccggtcgc cgaaattctc gaacgcgatt gcatcgcagc
     4021 cgccctcgca caacgcccgc gcgtcgcgca tggccgcgtc gatcaccgcg tccatcgacc
     4081 cgccgaaccg cggcgctccc ggcaacgcac cgacatgcac catgccgatg atggcattgc
     4141 gggagaagcg tttggcgaag tcgtcgcggg agagcatggg cttgatggat gaacgatgaa
     4201 ggatgaagga tgaacttgca atcagagatc agagatcagg agcgtaaggc cctgatctct
     4261 aatctcttgt cgcttgtctc tctcaagtcc ccttcatcct tataatcccc ctcccatgtc
     4321 cgatctgaag cttttcaaca cgctcacgcg cgagaaagag accttcgagc cgctcgttcc
     4381 cggcgaagtg cgcatgtact cgtgcgggcc caccgtctac aaccacctgc acatcggcaa
     4441 catgcgcacg ttcctctggg gcgatctgct gcggcgctac ctcgagttcc gcggcctgcg
     4501 cgtgaagcag atcatgaaca tcaccgacgt cgaggacaag atcatccgca atgccgcggc
     4561 cgccggaaaa gatctgacca cctacaccgc tccctacatc gagtcctttc acgattcgct
     4621 ggccaagttg cgtgtccgcc gcgccgatgc ctacccgcgc gcgaccgaat acatcccgca
     4681 gatggtggcc ctcgtccagc agttgaccga gcgcggccac acgtatgttg ccgacggctc
     4741 gacctacttc aaagtctcgt cactgcccgg ctacggcaag ctctcgcgcg tcgagatcga
     4801 ccagtcgagc gaatacagcc gcatcgaatc cgacgaatac gagaaagagt cagcgcgcga
     4861 cttcgttctc tggaaatcga agaaggacaa cgagccagcc tggcagacgc cgatcggcga
     4921 agggcgcccc ggctggcacc tcgagtgctc cgcgatgtcg atggatctcc tcggcgagac
     4981 cttcgacatc cacaccggcg cggtcgacct catttttccg caccacgaaa acgagatcgc
     5041 gcagagcgaa ggtgcgaccg gcaagccgtt cgtccgctac tggattcacg gcgagcacct
     5101 caacatcgat cagcagaaga tgtcgaagtc gctcggcaac atctatacgg tgcccgaggt
     5161 cgaggagatg gggtacgacc tcctggcgtt gcggtatgcg ctgctctccg tcccctaccg
     5221 caccaaactg aacttcacga tgcagtcgct cgacgacgcc aagaacgcac tgtcgcggat
     5281 cgaatcattc ctgctgcgtg tcgatgaagt gtcgcgctcg gccgcgcacg acgcgcgtca
     5341 ttccgacggc gccgcggatc agttgatcgg caagttcctc atcgatttcg agacggccat
     5401 ggacgatgac ctcaacaccg ccggcgccct cggcgcgctc ttcacgctga tccgcgaagc
     5461 caacaccgcc atcgacgaag gccgcatcct cgctggcgac gccgaggggc tgaagaccgc
     5521 ggtgatgaaa atcgatccgg tgttcgatct ttttccgaag cagcgcgaga acctcgacgc
     5581 ggagatcgaa tcgctcatcg ccgctcgcaa cgccgcgcgc aaagcccgta acttcgccga
     5641 aagcgaccgg atccgcgacg agctgctcgg cagggggatc ctcctggaag atacgcctgc
     5701 cgggacgcgc tggcggaagg gctgatgtct aacacctttt aaccacagag acacagaggt
     5761 cacagagtgg ggttagctct gtgtcctcag tgtctctgtg gttaaggctt ttccgcagta
     5821 accgcgtaaa attgagcgat gtcatcggag aacgatcctg cctatggcct ccgagtcgcc
     5881 ttcgacctgt tcgacgcagc cgaggcactc ttgcggcaga acatcctgcg agagtttcct
     5941 gagtcaaccg agcaggagat cgaag
//
LOCUS       DATLVW010000094         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_129456, whole genome shotgun sequence.
ACCESSION   DATLVW010000094 DATLVW010000000
VERSION     DATLVW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129456"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3057)
                     /locus_tag="VNN25_03185"
     CDS             complement(<1..3057)
                     /locus_tag="VNN25_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH90559.1"
                     /translation="MHVDDDPWQVFRFDLFASRILGPFSRSPFDLLLTAAALLAIAMT
                     LTRKRGTSIAAQIARSIVAAAAAAGYMALVRNLVDNSRISTMPDHILPVSAAQGVLLA
                     ALLLFGFAVVQITRHESSLKATLIGIGIIVIPIIATAAALPYMPKRAFVWIAGAMVIS
                     LLLHALTTNFTLRTFGSALLMVMIVYMPLQLFERSSARHFIADTYAPLVVGEAGQLRA
                     MIEDTLHNEFATTELSAILPDDYRKMNLEDLAYALWLRSDLSKWRVPAVIAVHDIFDH
                     PLSRFGVGLPQFSERRPEAGREVLQIGSLTRVLLHHDFDLTIGGIPIAEGSVHVVNPA
                     DPGATTFADVYRDFFEPSFNDTVTGLRPQREIVVYDRDGNAHGATTVRLPQSPASYFA
                     TLKSGNGVWIEENEGAGSAVYVRRTDDALYAFPLQVPTRGQQVRRAGGVAIWAIAFVL
                     AVIVMRSLPVIASVIRRAPRNLDFRTRTSLYLTAVVILPLIVFVLFVRAYLANRLEAE
                     YLDRGQTALTAAERVIEDYLASTTPTSHPEEVLNDDVLSWLARVIGHDLHLYRDDALI
                     ASSRRDLFAARVESERLPGDVYANIVVGGKQLAIRHSGPAQFIELYSPVNLVHGQNYT
                     MALPLIVQGRQIEGQVNDLATTIYMLLIFIVLAAIFVAYVAAGSVTRPVQALVGGARA
                     VARGDFDLDLRVPSDPDLGLLVTTFRDMAQSIRRQQNDLRHERDRLQTLLENINAAVV
                     VLDGEMNVSATNVGARRLFGDEVIFHDEVREFLTGHRHRRAEAKEIELTVDGNVRTFR
                     VSIVPLPGGDEEMLIAEDVTEILRSNRLEAWGEMARQVAHEIKNPLTPIQLTAEHLRA
                     VAEHDDPNLAKIVMTAVENILRQVVTLRETSKEFSDYASSRTVQRRPLRLRTLLEDIA
                     ASYAESGERGIRFHAEIQPSTPENFSGDARLLRGAIANLIENAFQAAPGGRVRMNSYG
                     VDSRVIIAVDDDGPGVPPELLPKIFDPYFSTKSSGTGLGLAITRKAI"
BASE COUNT          628 a         1042 c         1167 g          580 t
ORIGIN      
        1 gattgctttg cgggtgatgg caaggccgag gccggtgccg gaggacttgg tggagaagta
       61 ggggtcgaag atcttcggca gcagttcggg agggacgccc gggccatcgt cgtcgacggc
      121 gatgatcact ctggaatcga cgccgtagct gttcatccgg acgcggccgc cgggcgctgc
      181 ctggaacgcg ttctcgatga gattggcgat cgcgccgcgg agcaggcggg cgtcgcctga
      241 gaagttctcc ggagtggacg gttggatctc cgcgtgaaag cggatgccgc gttcgccgct
      301 ttctgcgtag ctcgcggcga tgtcctcgag caacgtccgt aagcggagcg gcctgcgctg
      361 aaccgttcgc gacgaggcgt aatcgctgaa ttctttcgac gtctcgcgca gcgtaacgac
      421 ctggcgaaga atgttttcga ctgcggtcat cacgatcttc gccaggttcg gatcgtcatg
      481 ctccgcaacc gcgcgcagat gctcggcggt caactgaatt ggcgtcagcg gattcttgat
      541 ctcgtgcgcg acctgccggg ccatctcgcc ccacgcttcc agccggttcg agcggaggat
      601 ctcggtgacg tcttcggcga tgagcatctc ttcatctccg ccgggtagcg gcacgatcga
      661 tacgcgaaac gtccggacgt tcccgtccac cgtcaactcg atctctttcg cctcggcgcg
      721 tcgatgccga tgcccggtca ggaactcgcg gacctcgtca tgaaagatca cctcgtcgcc
      781 gaagagtctt cgcgctccga cgttcgtcgc cgagacgttc atctcgccgt cgagcacgac
      841 gaccgctgcg ttgatgtttt cgagcaacgt ctgcaggcgg tcgcgctcat ggcgcagatc
      901 gttctgctgc cggcggatcg actgggccat gtcgcggaag gtcgtgacga ggaggccgag
      961 gtcggggtcg gaggggacgc ggagatcgag atcgaagtcg ccgcgcgcga cggctctcgc
     1021 gccgccgacc agcgcctgca ccggacgcgt gacgctgccc gcggcgacgt aggccacgaa
     1081 gatcgctgcc aggacgatga agatcaggag catgtaaatc gtcgtggcca gatcgttgac
     1141 ctgcccctcg atctgacggc cctgaacgat cagcggcagc gccatcgtgt agttctgtcc
     1201 gtgcacgaga ttgaccggcg agtagagctc gatgaattgg gcggggccgg aatggcgtat
     1261 ggccagttgc ttcccgccga cgacgatgtt cgcgtagacg tcgccgggca ggcgctccga
     1321 ctcgacgcgt gccgcgaata gatcgcgccg gctcgatgcg atcagcgcgt cgtcgcgata
     1381 aaggtgcaga tcgtgaccga tgacccgtgc cagccagctc agcacatcgt cgttgagaac
     1441 ctcttcggga tgcgacgtcg gtgtcgtcga cgcgaggtag tcctcgatca cccgctctgc
     1501 cgcggtgagc gcggtctgtc cgcggtcgag gtactccgct tccagccggt tcgccagata
     1561 cgcgcggaca aaaagcacga agacgatcag cggcagaatg acgaccgcgg tcaggtaaag
     1621 agaggttcgc gtgcggaagt cgagattgcg cggcgcacgc cggatgaccg atgcgatcac
     1681 cggcagcgac cgcatcacga tcacggcgag aacgaaggcg atggcccata tcgcgacgcc
     1741 gcccgcgcgc cgcacctgct gcccgcgcgt cggcacctgc aaagggaagg cgtagagcgc
     1801 gtcatcggtg cggcgaacgt agaccgccga tcccgctcct tcgttctcct cgatccagac
     1861 gccgttgcca ctcttgagcg tggcgaagta agaagccgga ctctggggca gacgaacggt
     1921 ggtcgcgccg tgcgcgtttc cgtcgcgatc gtagacgacg atctcccgtt gcggccgcag
     1981 gcccgtgacc gtgtcgttga acgagggctc gaagaagtcg cgatagacgt cggcgaaggt
     2041 cgtcgcaccc gggtccgctg gattcaccac atgcacgctc ccctcggcaa tcgggatccc
     2101 gccgatggtg aggtcgaagt cgtgatgcag cagaacgcgc gtcaggctgc cgatctgcag
     2161 cacctcccgc cctgcctcgg gacggcgctc gctgaattgc ggcagtccga cgccgaagcg
     2221 gctgagcggg tgatcgaaga tgtcgtgcac ggcgatgacg gcaggcacgc gccatttcga
     2281 caggtcgcta cgcagccaga gcgcgtaggc gagatcttcg agattcatct tccggtagtc
     2341 atccggcagg atcgcgctca gctcggtcgt cgcaaactca ttgtgcagcg tgtcttcgat
     2401 catggcgcgc aactggccgg cctcaccgac gacgagcggc gcgtaggtgt cggcgatgaa
     2461 atggcgcgcg ctggaacgtt cgaaaagctg cagcggcatg tagacgatca tcaccatcag
     2521 caacgcgctt ccaaacgtcc ggagcgtgaa gttggtcgtg agcgcgtgca gcaggagcga
     2581 gatgaccatc gcgcccgcga tccagacaaa ggcgcgcttg ggcatgtagg ggagcgccgc
     2641 cgccgtggcg atgatcggga tgacgatgat gccgatgccg atgagagtgg ccttgagcga
     2701 agactcgtgt cgcgtgatct gtacgacggc aaaaccgaag aggagcagcg ccgcgagcag
     2761 cacgccctgc gccgcggaaa cgggaaggat gtggtccggc atcgtggaga tgcgcgagtt
     2821 gtcgacgagg ttgcgaacga gcgccatgta tccggctgcg gccgcggcgg cgacgatgct
     2881 gcgcgcgatc tgcgccgcga tcgacgtacc gcgtttgcgc gtcagcgtca tcgcgatcgc
     2941 caggagggct gctgccgtga gcagtagatc gaacggtgaa cggctgaatg gcccgaggat
     3001 gcgcgaggcg aatagatcga agcggaagac ctgccacggg tcgtcgtcga cgtgcatcgg
     3061 caggagcgat gcgcgtgcag cgatgacgag aaggatggtc gctatcgcgg ggagtgggga
     3121 gtggggggcg ttcgagggag tcgggagtcg ggggtcgggg gtcggagaac cgaaaagcga
     3181 tcaactgcgg ggtgtggggg gtggggagtg gggggtcgga aaaccgaaag gcgatcaatc
     3241 tatagcgctc actccttcaa cggcacaaat agcagccgaa cagtcacagc tcccaccctc
     3301 gacactgcca ccctcacctc ccccaccccc caccccccac tccccactcc ccacgatagc
     3361 gaccatcctt ctcgtcatcg ctgcacgcgc atcgctcctg ccgatgcacg tcgacga
//
LOCUS       DATLVW010000095        14403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_86020, whole genome shotgun sequence.
ACCESSION   DATLVW010000095 DATLVW010000000
VERSION     DATLVW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14403
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86020"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VNN25_03190"
     CDS             <1..813
                     /locus_tag="VNN25_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXH90560.1"
                     /translation="DRLMLVRQYGVPYSRVVTNGTLLTEATIDKIIDSQITSLTISID
                     GSTKELYEEIRIGARFETVLANIELFKELRARRGTKLPHLQINHVLMERNVDHFEEFL
                     LLLDSIRPEQTDVRTIEPMTYVTGLESRDEAFYEKVRRVRPLYADYCSRNHIEDCGFI
                     RDQAGVIDLMTATGQRMTCRRPWDTLAIHANGDVHPCMSWTRPPAGNLAQQSFEEIWH
                     GENLAQLRREFEIAQPGIDCQRCTIKKTVSAGIYDDFFFNMVNKSEAWARPE"
     gene            810..1940
                     /locus_tag="VNN25_03195"
     CDS             810..1940
                     /locus_tag="VNN25_03195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90561.1"
                     /translation="MSDDDANQAVTGDMAAELDVIIVHYHAAASVREAVAALRLDANG
                     TNIRLNIVIADNGSTPEERAVLQSLDVHYLDSGRNAGYAGAVNIAFSATSSDVVVLMN
                     EDVNVLPGCLRALSTTLRSGASIAGPQFYWDRDCTFLLPCTEERTRGKERLKVGGKRS
                     LTHLARARSKWREHARRHWRAAEPLPTTALSGALLAFRRDTWSTIGPFDEGFSLYFEE
                     DDWLLRAVHAGLLTLYVPSAKAIHRHNTKLAREAKRVQWEAESFRRFGDRYYGERFMR
                     RLALAAKREQVVPDWQELAAEPTGAVRLELPRNCTWPLWIELTPSPLGFPAAMTRITD
                     PTAEIWQLPPLRGLEFLETFYLQLVDDAGRELGAYHVQRTPR"
     gene            2702..3889
                     /locus_tag="VNN25_03200"
     CDS             2702..3889
                     /locus_tag="VNN25_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HXH90562.1"
                     /translation="MDRLIWGITDQNPLQRRAQLAQPAPPPREAQNPATAAIESLADH
                     LRTTGNITFFLGRSIAYPPSNSDITSELLSQLGMIDAASEGLLPPLDLAASFFAVEHG
                     DISLENEVVDMISQRKGAMPESYGALARLQKALSAREAIRGKDQYKQLIISNNLDVLL
                     EKALLLEGVPFTRLVQLRSSSRVQVNTVSTVQKLASGKLQVCDPTGLVGDADPNDDAA
                     LGDLISRSGVRTVYVNGGIMCDDKAGKIPTATLVSAMVEPILFKLHGSQDVAKSCAIS
                     TEQYFDTLWRSIDQKCIPDQLATIISNTPLVLVGSRILDADFRLSYTLLRTSLESGTD
                     QDRFAVLPRAPGDGRDGSHRLTMEAWDKLSVMALKKYSIQMLDEREDVFFRRLADAFA
                     SHR"
     gene            3894..5984
                     /locus_tag="VNN25_03205"
     CDS             3894..5984
                     /locus_tag="VNN25_03205"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90563.1"
                     /translation="MSAAGCSTYKPYKGTASYQAEDAELFFGRRVDAEQLLARVLSAR
                     LTLLHAQSGAGKTSLVNATVIPGLERRGWTPVRILPHTDPMASARTATLGTVLPPPEA
                     EVAAIDRVLSALPAASEMTISELLHVYDSLDIRDLRKRSLIAPLGASHRRSLSEWGDL
                     VPQFCRLLRSTIDVDAFASHIAAVRQGGDAALATGNAIDGSTSLTTLRELLDSEHLAI
                     QYKTLLAYLDPPSKRLWPFLENLIQVYGERRPRFALLLVFDQFEEMFTRFVDPGTAAV
                     LSIADLPDWRLRYQFFEELQEVYCGRLPASTIDGQPSPGGELPSIRFLISMRSEYIAQ
                     LGSIREFAPEIDQSVFHLQLLSKEGALDAIQEPARLYGYRYGKECYERIIEDLTKEER
                     FVEPTHLSLICEKLWDEAGCERSANASPADVDGGIPEIGLDIYETRLKGAKGILRAFL
                     RDYLDGLRDADERTESLELLEPLITGTGTRNIVERSQLIDVPFGKTERRKRLLQGLVN
                     RTIVRIEIRLGGDFVEITHEFLISSIQEVMQEVFFANVEYQRLRAALQALRTLGSTYA
                     GTDIEGTLPEHSFTTLHQNREKIEWTPTATDLMFRASICYGRDRETISAWSRAFAETG
                     GDADVLAVLSRVERGDATRDILSAEELRKLNAVRQTTDLTPKQTAFVLRSQLAHGTPA
                     SEVDIRYWSMRAKA"
     gene            5981..7942
                     /locus_tag="VNN25_03210"
     CDS             5981..7942
                     /locus_tag="VNN25_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH90564.1"
                     /translation="MSKDPVLVIEEFLQRDQPQLRRNAVVALGTLRAMGCVDRLVDVT
                     LTDVDATVSEAAERELVDAIKSGNKRIFDLYEQSRGQPRQRNMLYALMGRLRAKGAAT
                     PSAHGDLRQALALFTYAYPARGWKFFFRVFPAALAGAVVGFVLTALILGAVAGAAGMH
                     QGVSSDLIVDALLGALLGVVGAELSAIRTTAYTLHYSRGIGALVEIAQALIFPAGFYT
                     LIAVVIISMRAAAASSAFMPLVPLLAMIAVSRIVTILAAGTARSPLKRFFYAAAAAAA
                     AGLITATISSWLLSTPQLWVWFLSGAAALAAAFASVDARVPSSARSRARALFAGSLIV
                     LIVIMLLDLALSAYLGSSKYRDVRLDALPIGAKLESTPTTTRQQILVDKVPFHVSLNA
                     RQTVRLTLQWQAPYNVVLMDSSHKQLETGNPFIDRMLTAGTYELYFGLGNAELASNNA
                     LSKTLETLGALFRRTNGIAQSSLPRPVIISAYLWNPSPTPSASVPAQTPFSGFLGDNA
                     TPPRTALAQSRVESGTKNARLGQISAALAQFREARRIDPNVVISPNDWNALCWFATAY
                     KHAAEVVDDACENAVALSPDDYHIRDSRGVARAMTGRTQGAIEDLNFFIAATDKADRR
                     AQRQRWVESMRAGRNPFTQEDLRSLPEQW"
     gene            complement(8119..9102)
                     /locus_tag="VNN25_03215"
     CDS             complement(8119..9102)
                     /locus_tag="VNN25_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXH90565.1"
                     /translation="MNGLLRSLRRVLIALAISVSLILILLATVVRQPSSRSLAYPPGP
                     RSSADILRRHVEFLTTTVRPRGADQPENLDRAAAYIGERFREAGARVSEQPFTERGRT
                     YRNIIADFGPLAGDKLVVGAHYDAFTSTGDLPGADDNASGTAGLLELARLLGVRPPQS
                     PVELVAFTTEEPPFFGSDEMGSAVHAAQLQRSGTRISGMICLEMIGYFHGQQTWSSPL
                     LGMMYPSKGDFVAVTGGWPDRELARDVKAAMRGAGGLDVYSFTGPREMLDASDQRNYW
                     SRGWRAVMVTDTAYLRNPNYHTKRDTYDTLDYEKTARVVDGVFNYVRGWAE"
     gene            complement(9341..11614)
                     /locus_tag="VNN25_03220"
     CDS             complement(9341..11614)
                     /locus_tag="VNN25_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXH90566.1"
                     /translation="MKRAVALLFASVLLPAAAFASESFILNIPQRFDASQEIGEVRIV
                     LGLSAAPAGSQLVVNGAPPLNLGDTGTFAGDNVSFTAAAGNHVLITYVPKSNFGADFC
                     AGANATPKNIPLRFSGAQDVVDYAMSSFVVGSPMVECSKVSKRVADTVATITPMADNV
                     APALDAIARGRLPLDVVLVLDKSGSMNDLPPDAGAGNVTTKVQILKSAVDAFVANWQM
                     LDQPTADGGDWSGDRIGVVFFDSAAVAQTLAGADAPANFFVRRSAGWNPIINDVDSLT
                     PGGSTSIGGGINTGMQQWVADPSNDATFVVVTDGIQNTAPLIQPTASGFLGLLPVAGL
                     PQELRKRFIPILTIGFGTPAAVDEDLLRNIAFETSGRSYISVNATTLFDDFALTLVSI
                     LKGNTASLAARRRATLTGPGPGPLQRVTVDDSARRVVFSLQWTPPLSNALDLEVLPPG
                     ASAPATPASSRTLPQASIQSFDIPPDHPGTWNVRVKRSKKADTKGDVPYSLNVFFLER
                     HLDYRLSVNPPHPKSGEKMTLRAEVSYGGKPLTRLPDGALRVQVQRPKAAIGSLLREL
                     TEITKRGQSGDVKTPYENKVASLPRAALARILPSDAEALTLKEEGRGFYSVALDPATI
                     PGQYALEAVLDWDDPRTGQVHREERVEQLVAVKADPAKTEIRATRSADRQSVQIAVVP
                     RDQFGNALGPGYESKVTARLTTKSGKLTGPTDTRVNGTYHFTLAGLPRDERPNAEISV
                     DGVVVGDLRTIAAKAKR"
     gene            complement(11740..14322)
                     /locus_tag="VNN25_03225"
     CDS             complement(11740..14322)
                     /locus_tag="VNN25_03225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HXH90567.1"
                     /translation="MSRLLAAVIFLAALPLAAARLPKSVIPDHYAISITPDLATAKFS
                     GQETIDVDIKEPVDTITLSSVGLELHDVVVASGSKLLNPTEITFDVPNEMASLKFGQT
                     LPPGKASMRISFNGALSEQLRGLYLSKTPRRNYAVTQFESTDARRAFPSFDEPAMKAT
                     FDITLVVDDGDTAISNGAIASDTPAGPGKHAIRFRTTTKLSTYLVAMLVGDFQCVSGG
                     VDDIPIRVCSVPGMQDLGKFALSAAEAAVSFYDKYYGIKYPFGKLDLIGIPDFEAGAM
                     ENAGAITFRETALLLDDKTASIERKRGVASVIAHEIAHQWFGDLVTMKWWDDIWLNEG
                     FATFMAPKPLMAWHPEWRVDLDQVGGTLNALSIDSTRSTRPIRQKAETRNEINALFDG
                     IAYGKTAAVLRMEETWVGEETFRDGIRAYLKKFSYGNAAAEDWWGTMTASTKQPFDAV
                     MKSFVDQTGAPLLHASESCGPDGTRTVTISQERMLTQSATPVPQSWSIPVCGHEAGAA
                     AAAPCRIIAKPADAFTLAFCEKPLYLSRNGAGYFVTDYPPSMRGALRGHVGEMPPSEQ
                     ISFQGNEWLLVRNMREDVSDYLSLARALPRPAERTLVAHISGNLGYLGRTLVDDRNRA
                     AWQSYVRETMRGFAPVTWEAPAGETPEQRIMRATVLDMLGIEGADPEVIAGAKRIAQQ
                     YMNDPSSVDAVIADRALPIAAINGDEAMFDQVLAHMKSAPTPEVAARYRQLIPMFRDP
                     KLIGRAVDFIYSDQVRSQDLPGMAAQLFFNPAARQIAWSAAQSHWEMLNKKIPTAIGA
                     ITGSVGSFCDPAMKQEAQAFFATHPAGSGERSLRRGLEQIDSCIAFKTAQQTSFDTAL
                     GVTK"
BASE COUNT         2795 a         4336 c         4375 g         2897 t
ORIGIN      
        1 gaccgcctca tgctcgtccg ccagtacggc gtcccctact cgcgcgtcgt cacgaacgga
       61 actcttctca ccgaggcgac gattgacaaa atcatcgact ctcagatcac ctcgctcaca
      121 atttccatcg acggcagcac gaaggagctc tacgaagaaa tccggatcgg cgcgcgattc
      181 gaaacggtcc tggcgaacat tgagttgttc aaggagttgc gggccagacg cgggacaaag
      241 ctcccgcacc tccagatcaa tcatgtcctc atggagcgga atgtcgatca tttcgaggag
      301 ttcctgctgc ttcttgattc gattcggccg gaacagaccg acgtacgcac catcgagccg
      361 atgacgtatg taacgggcct ggaaagcaga gacgaagcct tttacgagaa ggtgcgcagg
      421 gtgcgccctc tctatgcaga ctattgcagc cggaatcaca tcgaggactg cggtttcatt
      481 cgggaccagg ccggcgtcat cgatctcatg accgcgacgg gccagcgcat gacttgccgc
      541 cgtccatggg acacgctggc gatccacgcg aatggcgacg tgcatccctg tatgtcgtgg
      601 accagaccac cggccggcaa tctggcccag cagtcgtttg aagaaatctg gcatggagag
      661 aacctcgcgc aactgcggcg agagttcgag atcgcacagc ctgggatcga ttgtcagcgc
      721 tgcacgatca agaaaaccgt atcggccggc atctacgacg acttcttctt caacatggtc
      781 aacaagagtg aagcgtgggc gcgtcctgaa tgagcgatga cgatgcgaat caagccgtca
      841 ccggagacat ggcggcggaa ttggacgtca tcatcgttca ctatcacgcg gcagcatcgg
      901 tccgggaggc agtagcggcg ctgcggcttg acgcgaacgg aacgaacatt cgcctgaaca
      961 ttgtcatcgc cgacaatggc agtacccccg aagagcgcgc cgtgcttcaa tcgctcgacg
     1021 tccactatct ggattcaggc agaaacgcgg gatacgccgg cgcggtgaat atcgccttct
     1081 ctgcgaccag ctccgatgtc gtcgtcctga tgaatgagga tgtgaacgtt ctgccgggct
     1141 gcctgcgagc actgtcgaca accttgcgct ccggagcgtc cattgccggc ccgcagttct
     1201 actgggatcg cgactgcaca tttcttctgc cgtgcacgga agagcggacg cgcggcaaag
     1261 agcgattgaa agtaggcggc aaacgaagtc tgacgcacct ggcccgtgcc cgctcgaaat
     1321 ggcgggagca tgcccgtcgc cactggcgcg cggccgagcc actgccaacg accgcgctca
     1381 gcggtgcact tctggcgttt cggcgggata catggagcac aatcggaccg ttcgacgaag
     1441 ggttctcgct ctatttcgaa gaggatgact ggctgcttcg tgccgtccac gcaggacttc
     1501 tcactctgta cgtcccctcg gcaaaagcga ttcatcgtca caatacgaaa cttgcccggg
     1561 aagcgaaacg cgttcaatgg gaggccgaat cgtttcgacg gttcggagac cgctattacg
     1621 gcgaacgatt catgcggcgt ttggctctcg cagccaaacg ggaacaggtc gttccggatt
     1681 ggcaggagct cgctgcagaa cctaccggcg cggttcgtct cgagctcccg cgaaactgta
     1741 catggcccct ctggatcgaa ctgacgccaa gtcccctcgg atttccagcg gcaatgacgc
     1801 gcatcacaga cccgacggcc gagatctggc aattgccgcc gctaagaggc ctggagtttc
     1861 tggagacgtt ctatctgcaa ctcgtcgatg acgcaggacg agagcttggc gcgtatcacg
     1921 tccagagaac accgcgttga gcacgccatc gacaacgcga gccatctctg caaatcgcgc
     1981 aggtcggatt agagcgacag cctccggagc tataggccgc aggttcgaat cctgcggggc
     2041 gcaccaaggc ttccgttccg agctctcgcc tcgcgcagcg ttgaacgccg cgatccgccc
     2101 acccgtaact ccatcgcgcc tttcgcccga acggtggaag cttgacacat cggctgacac
     2161 ggagtaacgt caatttatca cccaaaaaga gagcggggct tgccggagcg ttcgtcgctt
     2221 tccagttctt tatcggcgtc attcagttag ctcatcgagg acgagaccca tggaatcgcg
     2281 gttgctagag cgcccatgac tgcattcaac gatttcgctg cccctctaac ggacggttac
     2341 gacgtcttct tgagttacag cagcttggac cttacatcag tcgaggcgat tgccactgca
     2401 cttaaagagg agggattgtc ggtttggttc gataagtgga gactgcggcc cggcgacacg
     2461 tggcagcctg aaatcgaaaa ggggattacc gcgagccgat ccatcgcggt gttcattggt
     2521 ccgaatggca tcagttcctg ggagcttccg gaggtacgag ccgcgatcga tctggcggtc
     2581 aaatcgaaga ccagagtcat tcctgtcctc ctgccgggtg cgaacgcaac aagcgatgga
     2641 attccgctga tgctgcgcaa ttttcaattc tgcgttatgg agcgcgatcg atcagactgc
     2701 ggtggatcga ttgatctggg gaatcaccga tcagaatcca ctccaaagac gtgcccaact
     2761 cgcccagccc gcgcctccgc caagggaggc gcaaaaccct gccactgcgg ccattgaatc
     2821 tcttgccgac catctgcgca ccaccgggaa catcacgttc tttctcggtc gaagtattgc
     2881 ctatccaccc tccaacagcg atatcacctc ggaactgctt tcccagttgg ggatgatcga
     2941 tgccgcgtcg gagggactat tgccgccgct cgatctggcg gcgagttttt ttgcggtgga
     3001 acacggagat atcagtctag aaaacgaagt cgttgacatg atctcgcagc gaaagggtgc
     3061 catgcccgaa tcatacggtg cgctggcgcg gcttcagaag gcgctctcgg cccgcgaagc
     3121 aatccgggga aaagaccaat acaaacagct gatcattagc aataacctcg atgtccttct
     3181 cgagaaggcg ctgcttctgg aaggagtacc gttcacccgg ctagtgcagc ttcgatcctc
     3241 atcaagagtt caggttaata ctgtttcaac ggtacagaag ctcgcatcgg ggaagctgca
     3301 ggtttgcgac ccgaccggct tggtcggcga tgccgatccg aatgacgacg cggcgctcgg
     3361 cgatttaatc agccgcagcg gcgttcggac agtttacgtc aacggcggca tcatgtgcga
     3421 cgacaaggcc ggtaagatcc cgactgctac tttggtgtcg gcgatggtcg agccaatcct
     3481 tttcaaactt catggttcgc aggatgtcgc aaagagctgc gccatttcta ccgagcaata
     3541 cttcgatact ttgtggcggt ccattgacca gaaatgcatt cccgaccagc tggccacaat
     3601 catttccaat acaccgcttg ttctggttgg ctcgcggatt ctcgatgcgg atttccgcct
     3661 gagctacacg ctcctgcgca catcgttgga gtcgggcaca gaccaggatc gcttcgctgt
     3721 actgccccgc gcacccggtg acggccggga cggatcgcac cggctgacca tggaagcctg
     3781 ggacaagctg agcgtcatgg cactcaagaa atacagtatc cagatgttgg acgagcgcga
     3841 agacgtgttc ttccgccggt tagcggacgc gttcgcatcc catcgctgac accatgtctg
     3901 ccgcgggatg ctcgacgtac aaaccgtaca aaggcaccgc cagttaccag gctgaggatg
     3961 ccgagctctt tttcggacgg agggtcgacg cagagcagct tctggcgaga gttctttcgg
     4021 cgcggttgac gctgctgcat gcgcagtcgg gtgccggaaa gacctcgctc gtcaacgcca
     4081 ccgttatacc gggactggag aggcgcggct ggacgcccgt ccgtattctc ccgcacaccg
     4141 acccaatggc ttccgcacga accgcgaccc tcggcaccgt cctgccgccg ccggaggcgg
     4201 aagtggccgc aatcgacaga gtgctgagtg cactacccgc cgcttccgaa atgacgatca
     4261 gcgaattact acacgtatac gactcgctgg acattcgcga tctgaggaag cgctcgttga
     4321 tcgccccgct tggtgcgtcg caccggcgat cactgagcga atggggagat ttggtgcccc
     4381 agttctgccg gctgctccga tcgacgatcg acgtcgatgc attcgcgtct cacattgctg
     4441 cggtcagaca aggaggcgac gccgctctgg ccacgggcaa tgccatcgac gggagcacct
     4501 cgctgaccac gttgcgggaa cttctggaca gtgaacatct cgccatccag tacaagacgc
     4561 ttctcgcgta tctcgatcct ccctcgaagc gtctgtggcc ctttcttgag aatctcattc
     4621 aggtctacgg cgagcgccga ccgcggtttg cgctccttct cgtattcgat caattcgagg
     4681 agatgttcac gcgattcgtc gatcccggga ctgcggccgt actttcgatc gccgacctgc
     4741 cggactggcg gctgcgatac cagttcttcg aggagttgca ggaagtctat tgcggacgcc
     4801 tgcccgcttc gacaatcgac ggtcagccgt cgccaggcgg cgagcttccc tccatacgct
     4861 tcctgatctc gatgcgcagc gaatacatcg ctcaattggg ttcgatccgg gagttcgcgc
     4921 cggagatcga tcaatcggtg ttccatctcc agcttctctc caaagaggga gcgctcgatg
     4981 cgatccagga gccggcccgg ttgtacggat atcggtatgg gaaggagtgc tatgagcgca
     5041 tcattgagga tctgaccaag gaggagcggt tcgttgagcc gactcacctt tcgctgatct
     5101 gcgaaaaact gtgggatgag gcggggtgtg aacgatccgc aaatgcgtcg cccgccgatg
     5161 tggacggagg cattcccgag atcggattgg acatctatga gacgcgtttg aaaggagcga
     5221 aaggaatcct ccgcgccttc ctgcgtgact acctcgacgg cctccgtgat gcggacgagc
     5281 gcaccgagtc gctcgagctt ctcgagccgc tgattaccgg cacggggacg cgaaacatcg
     5341 tcgagcgaag tcagttgatc gacgttccat tcggcaagac cgaacgcagg aagaggctcc
     5401 tgcaagggtt ggtgaacagg actatcgtcc gcatcgaaat ccgtctcggc ggcgacttcg
     5461 tagagatcac tcacgagttc ttgatttctt cgatccagga agtcatgcag gaggtgttct
     5521 tcgccaacgt agagtatcag cgcctgagag cggctttaca ggcgctccgg actctcggaa
     5581 gcacctacgc gggaaccgac atcgaaggta ctctgcccga gcactcgttc acgacgttgc
     5641 atcagaaccg cgagaagatc gaatggaccc caacggccac ggatttgatg tttcgggcca
     5701 gcatttgtta tggacgcgat cgtgagacga tctcggcttg gtcacgcgcg ttcgccgaga
     5761 caggtggcga tgccgatgtc ctcgccgttc tctcccgggt cgagcgcgga gacgcgacgc
     5821 gcgacattct atccgccgag gagttacgca agttgaacgc ggttcgacag acgacggatc
     5881 tcactcctaa gcagacggcg ttcgttttgc gtagccagct cgcgcacgga acgcctgcca
     5941 gcgaagtgga catccgctat tggtcaatga gggcaaaggc atgagcaaag atcccgttct
     6001 cgtgatcgaa gagttcctcc agcgcgatca accgcaactg cgacgaaatg cagttgtcgc
     6061 cctcgggact ctcagagcta tgggctgcgt cgaccggctt gtcgacgtaa ccctcaccga
     6121 cgttgacgct accgtcagcg aagcggcaga acgagagctc gtcgatgcga taaagagcgg
     6181 aaacaagcgg atctttgacc tctacgagca atcgcgcggt caaccgcgac agagaaacat
     6241 gctatatgca cttatggggc gcctgcgtgc aaaaggagcc gctacgccgt ccgcccacgg
     6301 tgatttgagg caagcgctgg cgctcttcac ttacgcttat cccgcgcggg gctggaaatt
     6361 cttttttcgc gtattccccg ccgcccttgc cggtgctgtg gttgggttcg tactaacggc
     6421 cctgattctt ggggctgtcg ccggagccgc gggtatgcat cagggcgtga gctcagacct
     6481 aatcgtcgac gctttattgg gtgctcttct gggcgtggtt ggagcggagt tatccgccat
     6541 acggaccacg gcatacaccc tgcactattc acgcgggatc ggcgcgctcg tagagatcgc
     6601 acaagcattg atctttccag cgggttttta caccctcatt gcggtcgtga tcatttcgat
     6661 gcgagcagcg gccgcctcgt cagctttcat gccgctcgtc ccgctgctcg caatgatcgc
     6721 cgtatcgcga atcgttacga ttctcgccgc gggaaccgct cgttcgcctc taaaacgttt
     6781 cttctatgcc gcggcggccg ccgcggccgc cggactgatc actgcgacga tctctagctg
     6841 gttgctatcg acgcctcaac tttgggtctg gtttctttcc ggcgccgctg ccttagctgc
     6901 tgcgtttgcg tcagtcgacg cacgcgtacc gtcgagcgca cgttctcgcg cgcgcgccct
     6961 ttttgcaggt tcgttgattg tgctcatagt cattatgctc ttagatctgg ccttgtcggc
     7021 ctatcttggg agctcaaagt accgcgatgt gagacttgat gcgttgccga ttggcgccaa
     7081 actcgaatcc acaccgacaa cgactagaca acaaattttg gttgacaagg tgccctttca
     7141 tgtatcgctg aatgctcgac aaactgtacg actgaccttg caatggcagg ctccctataa
     7201 cgtagtgcta atggattcga gccataagca attggaaacc gggaacccat ttatcgatag
     7261 gatgctaaca gccggcacat acgaacttta ctttggattg ggtaacgccg aactggcttc
     7321 aaataatgca ctgtcgaaga cgttggagac tcttggcgcc ctgttccggc ggacgaacgg
     7381 tattgctcag tcttcgttgc cacggcccgt tataatatcg gcatacctgt ggaatccttc
     7441 ccccacgcca tctgcatcgg ttcctgctca gactccattc tcgggattct tgggtgacaa
     7501 tgcaacgcct cctcgcacgg ctcttgccca gtcacgtgtc gaaagcggga caaagaacgc
     7561 gcgtttaggt caaatctctg cggcgcttgc tcaatttcgc gaggcgcggc ggattgaccc
     7621 aaatgttgta atttccccca acgactggaa tgcgctctgt tggttcgcaa ccgcttacaa
     7681 gcatgctgcg gaagtggtcg atgacgcctg tgagaatgcc gtggcactgt ctccggacga
     7741 ctaccatatt cgcgacagcc gaggagtggc aagggccatg acggggcgaa cacaaggtgc
     7801 cattgaagat ctgaacttct tcatcgctgc cactgacaaa gcggaccgac gggctcagcg
     7861 tcagcggtgg gtcgaaagca tgcgcgctgg ccggaacccg ttcacccagg aggatctgcg
     7921 atctttgcct gagcaatggt gacaagccct cgcggaggta aatcgcagcc ttgatctcgg
     7981 cgctcctctc cggctcgttc aggagcagag cccggactgt tacctcctcg attaaccttg
     8041 tcgaagcggt tgggggccgc tctaccgcta gaccgcaggt tcgactcctg cctcacgcac
     8101 caaccctcag gtgcacggtc actcagccca tcctcgcacg tagttgaata cgccatcgac
     8161 gacgcgcgcc gtcttctcgt aatcaagcgt gtcgtatgtg tctcgtttcg tgtggtagtt
     8221 tggattccgg aggtaggcgg tatcggtcac catcacggcg cgccagcctc gggaccagta
     8281 gttgcgttgg tcggatgcgt ccaacatttc acgagggccg gtgaagctgt agacgtcaag
     8341 gccgccagcg ccgcgcatgg ccgccttcac gtcgcgcgcg agctcacgat cgggccatcc
     8401 tccggttact gctacaaagt cgcctttgct cggatacatc attcccaaca gaggtgacga
     8461 ccacgtttgt tggccgtgaa agtagccgat catctccagg cagatcattc ccgatatccg
     8521 tgtcccggaa cgttgcaact gcgcagcatg aaccgcgctc cccatctcgt ccgatccgaa
     8581 gaacggcggc tcctcggttg tgaacgcgac caactcgacc ggactttgcg gcgggcgaac
     8641 accgagcagt ctcgccaact ccaaaagacc tgccgttccg ctggcgttat cgtccgcgcc
     8701 cggcagatca cccgtggacg tgaacgcatc gtagtgcgct cctacgacga gcttgtcacc
     8761 ggcaagcggt ccgaagtccg cgatgatatt gcgatacgtt cttcctcgct cggtgaaggg
     8821 ctgctcggag actcgagcgc ctgcttcacg gaatcgttcc ccgatgtatg cggctgcacg
     8881 atcgagattc tccggttgat ccgcacctcg cgggcgcacc gtcgttgtca agaactcgac
     8941 gtgccgccgc aagatgtcgg cagaggatct tggtcctggc gggtacgcca ggctgcgact
     9001 acttggctgc cgaacaactg tggccaacag gataaggatc aaactcacgc tgatcgcgag
     9061 tgcgatcagg acgcgccgca acgaccgcag gagtccgttc atcttactca tgagacggca
     9121 cggctccgcc aaaggttctc ctgcgatagt aattctcgcg acgaacgggc gtgattaatt
     9181 cagggtcttc cgcgctccgc gaaccaagtg accaccgccc caatcccaat tcccgctgca
     9241 aatccccaga ccatgtcgtt tgcgtctttt ccgaagatgt gaaacgcggc gagggcagcg
     9301 gccaggaggg ctagtccgaa cagcagaggg gctttgcgca ctatcgtttt gccttcgcgg
     9361 cgatggtacg cagatctcct accaccacgc catccacgct gatctctgcg tttgggcgtt
     9421 cgtcgcgagg gaggccggcg agcgtgaagt ggtaggtacc gtttacgcgg gtgtcggttg
     9481 ggccggtgag tttgccggac ttcgtggtca gtcttgcggt cactttcgat tcgtagccgg
     9541 gcccgagggc gttgccgaac tggtcgcggg gaaccacggc gatttggacg ctctgtcgat
     9601 cggcggagcg tgtcgctcgg atttcggtct ttgcggggtc ggctttcacg gcgacgagct
     9661 gttcgacgcg ctcttcgcga tggacctggc cggtgcgggg atcgtcccag tcgaggacgg
     9721 cttcgagggc gtactggccg gggatcgtgg ctgggtcgag cgcgaccgag tagaagccgc
     9781 gtccctcttc cttcagcgtg agggcttccg cgtcggacgg aaggatgcgg gcgagggccg
     9841 cccgcggcag tgacgccacc ttgttctcat acggcgtctt cacatcgccg gactgccccc
     9901 gcttcgtgat ctcggtgagc tcgcgtagca gcgatccgat ggctgccttc ggacgttgca
     9961 cttgaactcg tagcgcaccg tcgggcagcc tcgtcaacgg cttgccgccg tacgacacct
    10021 cggcacgcag ggtcatcttt tcgccgctct tcgggtgcgg cgggttgacg gagaggcggt
    10081 aatcgagatg gcgctcgagg aagaagacgt tcagtgaata cgggacatcg ccttttgtat
    10141 cggctttctt cgaacgcttg acgcgcacgt tccaggtgcc gggatggtcg ggtgggatgt
    10201 cgaatgactg gatcgaggcc tgcggcagtg ttcgtgatga cgcaggcgtc gccggtgccg
    10261 acgcgccagg cggcaacact tcgaggtcga gcgcgttgct aagcggcggc gtccactgca
    10321 gcgagaagac gacacgccgc gccgagtcgt cgacagtcac tcgctgcagt gggccgggtc
    10381 caggacccgt taatgtcgcg cggcggcgcg ccgccagcga tgcggtgttg cctttgagga
    10441 tgctgaccag cgtcagcgcg aagtcgtcga ataacgtcgt tgcattgacg ctgatgtagg
    10501 agcggcccga cgtctcgaag gcgatgttgc gcaggagatc ctcatcgacg gctgccggcg
    10561 ttccgaagcc gatggtcagg atcggaatga atcgcttgcg cagctcctgc ggcagtccag
    10621 caacgggcag gagtccgagg aatccgcttg cggtgggctg gatcagcggc gccgtgttct
    10681 ggattccgtc agtgaccacg acgaatgtgg cgtcgttcga agggtcggcc acccactgct
    10741 gcatcccagt gttgatgcca ccgccgatcg acgtcgatcc gccgggagtg agcgagtcga
    10801 catcgttgat gatcggattc catcccgcgc tgcgccgcac gaagaagttg gcgggagcgt
    10861 cggcgccggc cagcgtttgg gcaacggccg ccgagtcgaa gaagacgaca ccgatgcggt
    10921 cgcccgacca gtcgcctcca tccgccgttg gctgatcgag catctgccag ttggcgacga
    10981 acgcgtcgac agccgacttc aggatctgta ccttcgtcgt gacattgccg gcgccagcgt
    11041 ccggcgggag atcgttcatg ctccccgact tgtcgagcac gagcaccacg tcgagaggga
    11101 gcctcccgcg agcgatcgca tcgagtgccg gagcgacgtt atccgccatt ggcgtgatcg
    11161 tcgccacggt gtcggccacg cgcttcgata ctttcgagca ctccaccatc ggcgagccga
    11221 cgacaaacga tgacatcgcg tagtcgacga catcctgcgc accggagaag cgcaacggaa
    11281 tgttcttcgg cgtcgcgttc gcaccggcgc agaagtcggc gccgaagttc gacttcggga
    11341 cgtaggtgat gagcacgtga ttgccggccg ccgcggtaaa tgagacgttg tcccctgcga
    11401 acgtgccggt gtcgccgagg ttcagcggtg gagcgccgtt gacaacgagt tgcgagccgg
    11461 caggagctgc actgaggccg agcacgatgc gaacctcgcc gatctcctgc gaggcatcga
    11521 agcgctgcgg aatgttcagg atgaagctct cgctggcgaa tgccgccgca ggaaggagaa
    11581 cagacgcgaa gagaagcgca accgctcgtt tcatggtttc tcctgtccag actggaagga
    11641 aggattcaac cacgagcgga gggatctgtc acttcgcgcc ttcgcgtctt cgcgtgcgca
    11701 tccgtgtcac gcgaagccgc gaagaacgcg aaggaggtct tatttcgtta ctcccagcgc
    11761 ggtatcgaac gacgtctgct gcgccgtctt gaacgcgata cagctatcga tctgctcaag
    11821 tcctcggcgc agcgaacgct cgccggagcc ggcaggatgc gtcgcaaaga atgcctgcgc
    11881 ttcctgtttc atcgccggat cgcagaagct tccgaccgag ccggtgatcg cgccgatggc
    11941 ggtcgggatc ttcttgttca acatctccca atgcgactgc gcggcgctcc acgcgatctg
    12001 ccgggcggcg ggattgaaga atagttgcgc agccatgccg ggaagatcct gagagcgaac
    12061 ttgatcgctg tagatgaaat cgacggcacg gccgatgagc ttcggatcac ggaacatcgg
    12121 tatcagttgg cgatagcgcg cggcaacctc gggtgtcggt gcactcttca tatgagcgag
    12181 cacttgatcg aacatcgcct cgtcgccgtt gatcgcggcg atcggcagtg cgcggtcggc
    12241 aatgacggca tcgaccgatg agggatcgtt catgtactgc tgggcgatcc gtttcgctcc
    12301 cgcgatcacc tcgggatcgg cgccctcgat gcccagcatg tccaacacgg tcgcgcgcat
    12361 gatccgttgt tcgggcgtct caccggcggg cgcttcccac gtcaccggcg cgaagccgcg
    12421 catcgtctcg cgcacatacg actgccacgc ggcgcgattg cgatcgtcca cgagcgttcg
    12481 gcccaggtat ccgaggttgc cggagatatg agcgacaagc gtgcgctccg cggggcgtgg
    12541 cagtgcgcgc gccagcgaga gatagtccga gacgtcctcg cgcatgttgc gcacgagcag
    12601 ccattcgttg ccctgaaacg agatctgctc ggagggcggc atctcaccga cgtgcccgcg
    12661 caacgcgcca cgcatcgacg gcggatagtc ggtgacgaaa tagccggcgc cgttgcggct
    12721 gagatagagc ggtttttcac agaaagcgag cgtgaaggcg tcggccggct ttgcgatgat
    12781 ccggcatggc gcggcggcgg ctgcacccgc ttcatggccg cagaccggaa tgctccacga
    12841 ctgcggcaca ggcgtggccg attgcgtcag catccgctcc tgcgagatgg tcaccgtccg
    12901 tgtgccgtcc ggtccgcacg actccgaggc gtgcagcaac ggcgcgccgg tctgatcgac
    12961 gaacgacttc atcacggcat cgaacggctg cttcgtcgac gccgtcatcg ttccccacca
    13021 atcctcggcc gccgcgttcc cgtacgaaaa cttcttcagg taggcgcgga tgccgtcgcg
    13081 gaacgtctcc tcccccaccc aggtctcttc cattcgcagg accgcggcgg tcttgccgta
    13141 ggcgatgccg tcgaagagcg cgttgatctc gttgcgcgtc tccgctttct ggcggatcgg
    13201 gcgcgtgctg cgcgtggagt cgatgctgag ggcgttgagc gtgcctccga cctggtcgag
    13261 atcgacgcgc cattccggat gccaggccat gagcggcttc ggagccatga acgtggcgaa
    13321 gccctcgttc agccagatgt cgtcccacca cttcatcgtg acgagatcgc cgaaccattg
    13381 atgcgcgatc tcgtgagcga tgacgctggc cacgccgcgc ttgcgctcga tcgaagccgt
    13441 cttgtcgtcg agcagcaacg cggtctcgcg aaaggtgatc gcgcccgcgt tctccatcgc
    13501 ccccgcctcg aagtccggga tgccgatcag atcgagtttc ccgaaggggt acttgatgcc
    13561 gtagtacttg tcatagaacg acacagccgc ctcagcagcc gaaagcgcga acttgccgag
    13621 atcctgcatg ccggggacgg agcaaacgcg gatcgggatg tcgtcgacgc cgccgctcac
    13681 gcattgaaaa tcgccgacga gcatggccac gaggtacgtc gacagcttcg tcgtcgtgcg
    13741 gaaacggatg gcgtgtttgc ccggacctgc aggtgtgtcc gaggcaatcg caccgttcga
    13801 gatcgctgtg tcgccatcgt cgacgacgag cgtgatgtcg aacgtcgcct tcatggccgg
    13861 ctcgtcgaac gacggaaacg cgcggcgtgc gtcggtcgat tcgaattgcg tgaccgcgta
    13921 gttgcgccgc ggcgttttgc tcaaatagag cccgcgcagt tgctcgctca gcgctccgtt
    13981 gaagctgatg cgcatcgacg ccttgccggg cggcaaggtc tgcccgaact tgagcgacgc
    14041 catctcgttc ggcacatcga acgtgatctc cgtggggttc agcagcttgc ttccgctggc
    14101 gacaacgacg tcgtgcagtt ccagcccgac gctgctgagc gtgatcgtat cgaccggctc
    14161 tttgatatcg acgtcgatcg tctcctgccc gctgaacttc gcggtcgcga gatcaggcgt
    14221 gatgctgatc gcgtaatgat caggaatgac actcttcggc aaacgagccg cagcaagcgg
    14281 caaagcggcg agaaagatga cggcagcgag aaggcggctc atgtgggaag gatagcgcaa
    14341 ggagtgcagg cgtctcgcct gcggcgtcga ggcgtcctcg cctcggcgtg tgtatcgtgc
    14401 gcg
//
LOCUS       DATLVW010000096        11365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_375473, whole genome shotgun sequence.
ACCESSION   DATLVW010000096 DATLVW010000000
VERSION     DATLVW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375473"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1142)
                     /gene="nadA"
                     /locus_tag="VNN25_03230"
     CDS             complement(123..1142)
                     /gene="nadA"
                     /locus_tag="VNN25_03230"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXH90568.1"
                     /translation="MSTLFEELSRVRTYEYTAEDVREFEELHEEIRALKKERNAVIVA
                     HNYYRPEILKIADFVGDSLELALMASKVRDADVIDFCGVHFMAETAKIVNPSRTVLLP
                     NLAAGCQLADSAPAEDLRKRIAELKVEFADLAVVSYVNTTAAVKALSDVIVTSGNAAK
                     VVGTLPNKNILFVPDRNLARHVAEQVPDKNIIPWEGFCYVHQQITPHEVARIREADPE
                     IEILVHPECRADVAALADAVLSTSGMVKYAKERPAKRFLVVTECGLSDRLAMEVPDKT
                     FVKGCKLCTFMKVTTLEDVRDSLKYMRYEIDIPEDIRVPAERALRRMFAATEGGTLRS
                     EIAPK"
     gene            complement(1179..1448)
                     /locus_tag="VNN25_03235"
     CDS             complement(1179..1448)
                     /locus_tag="VNN25_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HXH90569.1"
                     /translation="MSEPWRVVFTKAAQKDARKVAESGLKGKTEALIELLREDPFRTP
                     PPYEKLIGDLAGAFSRRINRQHRLVYSVHQAERTVKVLRMWTHYE"
     gene            complement(1445..1687)
                     /locus_tag="VNN25_03240"
     CDS             complement(1445..1687)
                     /locus_tag="VNN25_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXH90570.1"
                     /translation="MKRVSAKQAQGILESLIEEAAAAHVTIGITGERAEGVLVPLSDW
                     QAIQETLYILSVPGLRESIREGMAEPLDACSETLDW"
     gene            complement(1715..2035)
                     /locus_tag="VNN25_03245"
     CDS             complement(1715..2035)
                     /locus_tag="VNN25_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXH90571.1"
                     /translation="MDDEPVGDNDERNVEPNDEPVEIPITDTLDLHPFRPGEIRDVAS
                     EYLIAARDRGFRQVRLIHGRGIGVQRENIRKLLASLDYVEAFHDADGSGGGWGATVVV
                     LRQV"
     gene            2090..3187
                     /locus_tag="VNN25_03250"
     CDS             2090..3187
                     /locus_tag="VNN25_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HXH90572.1"
                     /translation="MVLFNHATREMTAKIVYYGPGLCGKTTNLMVIFDKLDPKQKGKM
                     LSLATKTDRTLFFDLLPVDIGKVGSFNLKIQLYTVPGQVFYNETRKLVLKGADAVVFV
                     ADSQPSMVESTRESFGNLLENLEQNNIDPNDTPIVIQFNKRDIPGVLPIEELQERLGF
                     EGYPYTEAAAIKGDGVMETFKLISKITAKHLYTRLQGKPSPPEKAKADSVRVKGKAIP
                     EPELEPEPEPEPEPSPTPQVNPFADSVPFPESQIDSSYERMEEVSLESLLTDGRERPA
                     TISGNVPIPIDPDDVEELDAATLVSAEETPSPDVAVAEVPDANASGVEAKVRLLEAQM
                     DSMRAGHRAELESILRELDALSARVRDRLHS"
     gene            3255..4043
                     /locus_tag="VNN25_03255"
     CDS             3255..4043
                     /locus_tag="VNN25_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90573.1"
                     /translation="MRRTLSAVLMLSIALAGCRAKEAYDKAKISQDLDKHSTLDLMKD
                     VSKDKYTPPSDGKLTDGQVQMYLKVREHEKVIAQVAKQEAQQHADAAKKAGEKSIAGM
                     MEGFKTIGSAADMLTADIRAAKDLHYNTQEYLWVKGQVMAASTATLTSKMAEATNASF
                     DSAHAQMQKSYDEAKDDQTKKMYKEMLDNYDKSRAEMKQSSAANISPAIAYNQQLLAK
                     YDGALNAITNEVAKYEDKPGDAQKSMDELQKNLDKTVADAKKTQ"
     gene            4187..5320
                     /locus_tag="VNN25_03260"
     CDS             4187..5320
                     /locus_tag="VNN25_03260"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXH90574.1"
                     /translation="MTTRRFPVHLACSRCDATFEIRALMNLCPNDSAPLLAQYDLAPA
                     RELRDEIRSRPATMWRYREVLPADDDREIVTLGEGITPLLRSTQFENVWIKDESKNPT
                     RSFKSRGMAAAVTMAKRLGAKTLAAPTAGNAGAALSAYGARAGLPVFVAMPRDTPRSI
                     IDECRGYGAEVELVAGVITDAGKRVAQYIAANGGFDLSTLKEPYRVEGKKIMGYELLE
                     DLGRLPDVVLYPTGGGTGLIGMWKAFDEMERLGWIGSERPRMFSVQSSGCAPVVRAFN
                     EGLETAPEWESPETGAWGLRVPRAVGDRLMLRALRDSKGGAIAVDESQIESAAAELRH
                     TEGIDAGPESGAALLALRELQKTGAISSSDTVVLFSTGGNKYQ"
     gene            complement(5330..5875)
                     /locus_tag="VNN25_03265"
     CDS             complement(5330..5875)
                     /locus_tag="VNN25_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90575.1"
                     /translation="MFQNHVESVRRCLIDGGTGRRFSSADFGKISAGVRKTLLLGAKV
                     LLRDQKVLLQEQMILLGEETILLQEEMHLLQEEMNLLREEMNLLGDQKVLLGEEKVLL
                     GEEKVLLGDQKVLLGEEKVLLGDQKVLLRDQKVLLEEENVLIQEENVLIREERVLIRE
                     EKVLLQEEKILVLIRKALPGA"
     gene            6012..11354
                     /locus_tag="VNN25_03270"
     CDS             6012..11354
                     /locus_tag="VNN25_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90576.1"
                     /translation="MNIRSAVLLCAGLAFGGQALAQCPASGAPVPQSPIGTNQSANVP
                     ITFSWTPSTASGVTGYDVFASTGSPSATLVCSAGSTASSCSGPAAGLTPGTYNWIVRA
                     NIPNCASGLQSTGKQFTVGCLTSSPTIQSPSDGSQNFFANQALTWSAVSGADMYDVYF
                     GVSGSGACTGTPQFTTSNTSFNPPTLAASTTYEWRVVAKKTTATTCPTTTSGCATFKT
                     AAAACNPPGSFNLTSPSDKSATASTPTLTWSASSGTDKYLLHISTQNPPAPSASDPIV
                     NGSSTSFTFGQALPQGTYYWSVDAYPPNCTTVKTSSNVFSFTVAATAACPTAPAALIT
                     PANNTTVDSPVSFDWTDVSGATSYKVIASINGAAAAILAVTRDSHYDGSLPAGASVDW
                     WIEADADNCTPVTSAHSHFNVTNAGVCPSSPGSATLLSPANGATGLTSPVTFQWSVVP
                     GATNYVVWGVTTSAVSSNERFVIGKTPGTHITTAVPQGGLTWYVEADFGDCPTATYSK
                     SSTLSVATAAVCSSIPAALLSPVNGATGLTSPVTFQWTAVSGATGYKLFIAQGNDAGD
                     LAGTTTSTSLTTLVPGGSVTWWIVTTFAGCPDIVSAKSSFTSGTQTTCGATATLNSPA
                     DGATVSSPVTLSWSTVNGASGYRLWIAVNGGSPILAARSTTAAGTSQQVQLTSGTDEW
                     YVETLFSNGCDSTFSAHRHFTVSAAANCDTHKAATLTAPIGGAVVSNPVTFNWSSTDS
                     SALQYRVWVSLNGDPFSDIGFSKDTKLQHDFGTNTGNGQWFVETLFENCPPVLSAKAS
                     FVIPTPGCGTAGPQLLSPPDGATASAPVTLVWSAVTNATDYRVIWTLNGNDMPVLQTT
                     DTSITHIVPPGLVEWRVQAVFDGCPSTRSTKSRFTIPAAQTCPPDGPVLTAPADGATN
                     VDSPVRFDWNPVSSAIGYAVFVRHNDGAPTELADTTTRTAVTKAIPEGQTEWWVVAFF
                     NGCPPAESKHAIFTIPETNCDNPRPILFTPADNSTGLASPVHFEWSRIDNATKYKVWA
                     AVDDEDASVIGTTTVNKLTVGVPSGTIHWYVEAFFDSCPSLDSATGKFTVRKSAPPCD
                     TPDRPKAAAPAQVASNSSYTVHWSAVLNATSYELQESSTADFAAATTQVVTGLSASFV
                     HTAAANPQKWRYRVRAISSCDDERGPYSRIVIVTVLPDAPQKQTSVEIGTQSTIRQTI
                     FIPGHTPPLPFTARTDKPWASVTPSSGTLGTQGVTLTIVSDPTALKLGTNTATVILTF
                     SAATKGGVSASDTTTPPGSVPVSVTTVTPVSSGGKSGPLPNSLIIPAVGHATGANSSL
                     FESDLRIANITAQAMKYQLNFTLTATDGTQSGQTTTIQVDPGSTMALDDILTSFFGDG
                     SDGSSALGVLEIRPLTTTTQSGAPSVQTVASSRTYNSTPTGTFGQFIPAIPFSQFIAQ
                     SQDSSPNPIITLQQIAQSAAYRTNFGIVEGAGEPANVLVHVFNNQGIEVVAPIPLSLL
                     PGEHQQRNLLAENGITLTDGRFEVEVVSATGKVTAYASVIDNLTNDPLLVFPVLKGAE
                     SATRYVIPGVADINNGFASWRSDIRLFNPGSTPVTATLTYFPQPGNTGASGPKQVTIP
                     AGQVMPIDNALQNLFTLTNSGGSMLVTTPSSSKLVVTARTYNQTTNGTYGQFIPAVTP
                     AGSVGLSDNRVLQLLQLESSDKMRTNLGFVETSGNPVTIEVSAIPSDSKVAAKTQIPL
                     AANQFLQVSLAQFGLGTAYNARVTVKVLSGTGRVTAYGSVIDAATQDPTYVPAQ"
BASE COUNT         2301 a         3869 c         3174 g         2021 t
ORIGIN      
        1 tgtccgggaa aagacgccgg aagcggccgc gaagcgcgta gcggaagcga tcatgcgcga
       61 aaccgaagcc ggcacctaac tgacgcgtca tcggtccggc tgtcggcgca acgttcgttc
      121 tcctacttgg gggcgatctc gctgcgaagt gttccccctt ccgttgcagc gaacatcctc
      181 cgcaacgctc gctccgccgg gactcggatg tcctcgggga tgtcgatctc gtagcgcatg
      241 tacttgagcg aatcgcgcac atcctcgagt gtggtcacct tcatgaacgt gcagagcttg
      301 cagcccttga cgaaggtctt gtccggcact tccatcgcca agcggtccga gaggccgcat
      361 tcggtgacga caaggaaacg tttcgccgga cgctccttcg cgtacttcac catccccgaa
      421 gtggagagca ccgcgtcggc cagcgcggcc acgtcggcgc ggcattcggg atgaacgagg
      481 atctcgatct cgggatcggc ttcgcggatg cgcgccacct catgcggcgt gatctgctga
      541 tgcacgtagc agaacccttc ccacggaatg atgttcttgt ccggaacctg ttcggcgacg
      601 tgacgcgcca gattgcgatc gggtacgaag aggatgttct tattcggcag cgtaccgacg
      661 actttcgccg cgttccctga cgtgacgatg acatccgaaa gcgccttcac cgccgcggtg
      721 gtgttgacgt agctgacgac ggccagatca gcgaactcca ccttcagttc cgcgatccgt
      781 ttgcgcagat cctcggcggg cgctgaatcc gcgagctggc atcccgcggc aagattcgga
      841 agcagcacgg tccgcgacgg attcacgatc ttcgccgtct ccgccatgaa gtgaacgccg
      901 cagaagtcga tgacgtcggc atcacgaact ttcgaggcca tcaacgccag ctccagcgaa
      961 tcgccgacga agtccgcaat cttcagaatc tcgggccggt agtagttgtg cgcgacgatg
     1021 acggcgttgc gttccttctt cagggcccgg atctcttcgt gcagctcttc gaactcacga
     1081 acatcttccg ccgtgtactc gtacgtccgg acgcggctca gttcttcgaa caaggtagac
     1141 atgtcttttc gaactgtagc ggattcagcg ccatttcctc attcgtagtg cgtccacata
     1201 cgcaagacct ttacggttcg ctcggcttga tgaactgaat agacaagtcg atgctggcga
     1261 ttgatgcggc gcgaaaacgc tccggcgaga tcgccgatca gcttttcgta aggtggcggg
     1321 gtccgaaacg gatcttcacg cagaagctcg atcaacgcct cggtctttcc cttcaaacct
     1381 gattctgcaa ctttcctggc gtccttttga gcggccttcg tgaaaacgac gcgccatggt
     1441 tcgctcacca gtccagcgtc tccgagcacg cgtcgagcgg ctcagccatt ccttcgcgaa
     1501 tcgactcacg caggcctgga acagaaagga tgtagagcgt ctcctgaatc gcctgccagt
     1561 cggaaagagg tacgagtacc ccctccgccc gctctcccgt gattccgatc gtcacgtgtg
     1621 cggcagcggc ttcctcaatg agactctcca ggatgccctg cgcctgcttt gcactaactc
     1681 gcttcatgcc agtcatccta acatcgccgt ccggtcaaac ctgccgcagc accaccaccg
     1741 tcgcgcccca ccctccccca ctcccgtcgg cgtcgtggaa ggcttccacg tagtcgagtg
     1801 aggccagcag cttgcgaatg ttctcccgct gcacgccgat cccgcggccg tggatcaggc
     1861 gcacttgccg gaagccgcga tcccgcgctg cgatcaggta ctccgacgct acatcgcgaa
     1921 tctcgccggg ccggaacgga tgaagatcga gcgtatcggt gatcggtatc tcaaccggct
     1981 catcgttcgg ctcaacgttc ctctcatcgt tgtcgcccac cggctcgtca tccatgccgc
     2041 gattctatgt gcgatacttt cgctcccgaa accgatccga gaaaaaccga tggtcctgtt
     2101 caatcacgcg acccgcgaaa tgaccgccaa gatcgtttac tacggtcccg gcctttgcgg
     2161 aaagacgacc aacctgatgg tcatcttcga caagctcgac ccgaagcaga agggaaagat
     2221 gctgtcgctg gcgacgaaaa ccgatcgcac gctcttcttc gaccttctgc cggtcgacat
     2281 cggcaaagtc ggctcgttca atctcaagat ccagctctac accgtgcccg gccaggtgtt
     2341 ctacaacgag acacgcaagc tggtgctcaa gggcgccgat gccgtcgtct tcgtcgcgga
     2401 ctcgcagcct tccatggtcg aatcgacccg cgagagtttc ggcaatctgc tggagaacct
     2461 cgagcagaac aacatcgatc cgaacgacac gccgatcgtc atccagttca acaaacgcga
     2521 catccctggt gtcctgccca tcgaggagct ccaggagcgc cttggattcg aaggctatcc
     2581 gtacaccgag gccgccgcga tcaagggcga cggcgtgatg gagacgttca agctcatctc
     2641 caagatcacg gccaagcatc tctacactcg gttgcaggga aaaccctcgc cgcctgaaaa
     2701 agcgaaggcc gactccgtcc gcgtgaaggg gaaggcgatt ccggagcccg agctggagcc
     2761 cgaacccgaa cccgagccgg agccgtcccc gacgccgcag gtcaatccgt ttgccgactc
     2821 cgttcctttc cccgagtcgc agatcgatag cagctacgag cgcatggaag aggtctcgct
     2881 cgaatcgttg ctcaccgacg ggcgggaacg tccggccacg atctcgggca acgtgcctat
     2941 tccgatcgac cccgacgacg tcgaagagct cgacgcagcc acgctggtat cggccgagga
     3001 gactccttca ccggatgttg cggtagccga agtacccgat gcaaatgcca gcggcgtgga
     3061 agcgaaggtt cgtctgctcg aagcgcagat ggactcgatg cgcgccggcc atcgcgccga
     3121 gctggagtcc atccttcgcg agctcgacgc gctgagcgcg cgcgtgcgtg atcgcctcca
     3181 ttcatgaacc aggcggttgc caattcggcc cgctgtccct aaaatcacgg tccatcttta
     3241 tttggaggat accaatgcgc cggacacttt ccgcggtact catgctgtca atcgcactcg
     3301 caggatgcag ggccaaagag gcctacgaca aagcgaagat ctcccaggat ctcgacaaac
     3361 actcgaccct ggacctgatg aaggacgtct cgaaggacaa gtacacgcct ccgtcggacg
     3421 gcaagctcac cgatgggcaa gttcagatgt atctcaaggt gcgcgagcac gaaaaggtca
     3481 tcgcgcaggt tgcgaaacag gaagcgcagc agcacgccga tgccgcgaag aaagcgggcg
     3541 agaaatcgat cgccggcatg atggaaggct tcaagacgat cggctccgcc gcggacatgc
     3601 tcaccgccga catccgcgcc gcgaaagatc tccactacaa cacgcaggag tacctctggg
     3661 tcaaaggcca ggtgatggca gccagcaccg ccacgctgac gtcgaagatg gccgaggcca
     3721 cgaatgcgtc gttcgactcc gcgcacgcgc agatgcagaa atcgtatgac gaagccaaag
     3781 acgatcagac gaagaagatg tacaaagaga tgctcgacaa ctacgacaag tcgcgcgccg
     3841 agatgaagca gagctccgcc gcgaacatct ctcccgccat cgcctacaac cagcagctct
     3901 tagccaaata cgacggcgcg ctcaacgcca tcacaaacga agtcgccaag tacgaagaca
     3961 aacccggcga cgcgcaaaag tcgatggacg aattgcagaa gaacctcgac aagacggttg
     4021 ccgacgcaaa gaaaacccag tagcaagatc aaatcgatcg tgaaaggccg ccacctcggc
     4081 ggcctttttt catctgcgcc catctgcgta tatctgcgga tgaaaccttt ccgcagatga
     4141 cgcagatgta cgcagatgaa cccgccctcg cgatacgatt cgaaacatga ccacccgccg
     4201 ctttcccgtc catctcgcct gctcccgctg cgacgctacc ttcgagattc gtgcgctcat
     4261 gaatctctgt ccgaatgaca gcgcgccgct tctcgcgcaa tacgacctcg cgcctgcgcg
     4321 tgagctgcgc gatgaaatcc ggagccgtcc ggccacgatg tggcgctatc gcgaggtcct
     4381 tcccgccgat gacgatcgcg aaatcgttac cctcggcgaa ggcatcaccc cgctgctccg
     4441 atcgacgcaa ttcgagaacg tctggatcaa ggacgagtcg aagaatccga cgcgctcctt
     4501 caaatcgcgc ggtatggctg cggccgtgac gatggccaaa cgcctcggcg cgaagacact
     4561 cgccgcgcca accgcgggca acgccggcgc ggcgctctcc gcctacggcg cccgcgccgg
     4621 actgcctgtc ttcgtggcga tgccgcgcga cacgccgcgg tcgatcatcg atgaatgccg
     4681 cggctatggt gccgaggtcg agttggtcgc cggcgtgatc acggatgcag gcaagcgcgt
     4741 cgcccagtac atcgctgcga acggcggctt cgatctctcg acactgaagg aaccgtaccg
     4801 cgtcgaagga aaaaagatca tgggctacga gctcctcgaa gacctcgggc gccttcccga
     4861 cgtcgtcctc tatcccaccg gcggcgggac aggcctcatc ggcatgtgga aagcgttcga
     4921 cgaaatggaa cgcctcggat ggatcggctc cgagcgcccc cgcatgttca gcgtgcagtc
     4981 gagcggttgc gcgccggtgg tccgcgcgtt caacgaaggt ctcgaaacgg cgcctgagtg
     5041 ggaatcgccg gagaccggcg cctgggggct gcgtgttccg agggccgtcg gcgaccgcct
     5101 gatgctccgt gctctgcgcg attccaaagg cggcgccatc gccgtcgacg aatcgcagat
     5161 cgaatccgcc gccgcggaac tgcgccacac cgagggaatc gatgccggcc ccgagagcgg
     5221 cgcagcgctg ctcgctctgc gcgaattgca gaaaacaggc gcgatctcct ccagcgacac
     5281 cgtcgttctg ttcagtacgg ggggaaacaa gtaccagtga cggggttttt caggccccgg
     5341 gcaaagcttt tctgatcagg accaaaatct tttcctcctg gagtaagact ttttcctccc
     5401 gaatcaaaac tctttcctcc cggatcaaaa cgttttcctc ctggatcaaa acgttttcct
     5461 cctcgagcaa aactttttgg tccctgagca agactttttg gtccccgagc aaaacttttt
     5521 cctccccgag caaaaccttt tgatccccga gcaaaacttt ttcctccccg agcaaaactt
     5581 tttcctcccc gagcaaaacc ttttgatccc cgagcaaatt catttcctcc cggagcagat
     5641 tcatttcctc ctggagcaaa tgcatttcct cctggagcaa aatcgtttcc tccccgagca
     5701 aaatcatttg ctcctggagc aaaacttttt gatcccggag cagaactttc gcgccgagga
     5761 gcagagtctt tcggacgccg gctgagatct ttccaaaatc cgcactcgaa aaccgcctgc
     5821 ctgtgccccc gtcgatgagg cacctgcgaa cgctttctac atggttttgg aacatccctt
     5881 cgcttcggat gtggccgctt cggcgaagga gaccgtgaga gaagtcacta cgttgcgtac
     5941 agactggcgt tacagtcacg tcaacgtcag acgggttggg ttcgtctaac cgggacctta
     6001 ggggaatgac catgaacatt cgatctgctg tactgctgtg cgccggactg gcgtttggcg
     6061 ggcaggcgct cgcgcagtgt ccggccagtg gcgcaccggt accgcaatca cccatcggaa
     6121 cgaatcagag cgcgaacgta ccgatcacct tcagctggac gccctcgacg gcatccggcg
     6181 tcaccggata tgacgtcttc gccagcacgg gcagcccttc cgcaacgctc gtttgctccg
     6241 ccggctcgac ggccagttcc tgcagcggtc cggcggccgg tcttaccccc ggtacgtaca
     6301 attggatcgt ccgcgccaac atcccgaact gcgcctccgg actgcagtca acaggcaagc
     6361 aatttaccgt cggctgcctc acgagctctc caaccattca gtcgccgtcg gacggttcgc
     6421 agaacttctt tgccaatcaa gcgctgacgt ggagtgccgt cagtggcgcc gacatgtacg
     6481 acgtctactt cggcgtgtcg ggatcgggcg cctgcacagg cacgccgcaa ttcacgacgt
     6541 cgaataccag cttcaatcca cccacgctgg cggccagcac gacctacgaa tggcgcgtcg
     6601 tagcgaagaa aacgactgcc actacttgtc caaccaccac cagcgggtgc gcaacgttca
     6661 agaccgccgc cgctgcctgc aatccgcccg gctcgttcaa cctcacctct ccgtccgaca
     6721 agagcgcgac cgcttcgacg ccgacattga cctggtccgc ctcctccggt acggacaagt
     6781 atcttctcca catcagcacg cagaatccgc cggctccgag cgccagcgat ccgatcgtca
     6841 acggcagctc gacgagtttc acattcgggc aggcgctccc ccagggaacc tactactggt
     6901 cggtcgatgc ctatccgccg aactgcacga ccgtgaagac ttccagcaat gtcttcagct
     6961 tcaccgtcgc cgcgacggcc gcctgtccga cggcgcccgc cgctctcatc acgcctgcga
     7021 acaacaccac cgtcgactcc ccggtgagct ttgattggac cgacgtctct ggtgcgacct
     7081 cctacaaagt gatcgcttcc atcaatggcg cggcggcggc catcctggca gtcacacgcg
     7141 actcccacta cgatggcagt cttcccgccg gtgcatccgt ggactggtgg attgaagcgg
     7201 atgccgacaa ctgcacgccg gtcacgtcgg cgcattcgca cttcaacgtc accaatgcgg
     7261 gcgtctgtcc gagcagcccc ggctcggcaa cgctcctctc accggccaac ggcgccacgg
     7321 gacttacctc tcccgtcacc ttccaatggt cagtcgtccc gggtgcgaca aactatgtcg
     7381 tctggggagt aaccacgagc gccgtcagca gtaacgagcg attcgtcatc ggcaagacgc
     7441 ccggaacgca catcaccacc gcggtgccgc agggcggcct gacctggtat gtcgaagccg
     7501 atttcggcga ctgcccgacc gcgacgtact ccaagtcctc cacgctctcc gtcgccacag
     7561 cggcggtctg cagcagcatt cccgcggcgc tgctctcgcc ggtcaacggg gcaactggcc
     7621 tgacctcacc ggtcacgttc caatggaccg ctgtaagcgg agccaccggc tacaagctgt
     7681 tcatcgcaca aggcaacgac gccggcgatc tggccggcac gacgacctca acctctctga
     7741 cgacattggt gccgggcggc tccgtgactt ggtggatcgt gacgacattc gccggctgcc
     7801 cggacatcgt ctccgcgaag tcatccttca ccagcggcac gcaaacgacg tgcggcgcca
     7861 ctgccacgct caactcccca gccgacggcg cgacggtcag ctcgcctgta acgctctcct
     7921 ggagcaccgt caacggtgcg agcggctatc gcctctggat tgccgtcaac ggcggctctc
     7981 cgatccttgc ggcgcgcagc acgacggctg cgggcacgtc gcagcaggtg caactgacga
     8041 gcggtacgga cgaatggtac gtggagacgc tcttcagcaa cgggtgcgac tcgaccttct
     8101 ccgcgcaccg ccacttcacc gtcagcgccg cggcgaactg cgacacgcac aaagcggcca
     8161 cgctcaccgc accgatcggc ggcgccgtgg tgtcgaatcc ggtgaccttc aactggtcat
     8221 caaccgattc ctccgccctc caataccgcg tctgggtctc gctcaacggt gacccgttct
     8281 ccgacatcgg tttctcgaaa gacacaaagc tccagcatga cttcggaacg aacaccggca
     8341 acgggcagtg gttcgtcgag acgctgttcg agaactgccc gccggttttg tccgcgaaag
     8401 cgagcttcgt catccccaca ccaggctgcg gaaccgcggg tccccaactc ctctcgccgc
     8461 ccgatggagc gacggccagc gcaccggtca cgctggtctg gagcgcggtc accaacgcca
     8521 ccgactatcg cgtgatctgg acgctcaacg gcaacgacat gccggtgctg cagacgacgg
     8581 atacgtcgat cacgcacatc gttccgccag gtctggtgga gtggcgtgtg caggcggtct
     8641 tcgacggttg cccgtcgacg cgctcgacga agtcacgttt cacgatccct gcggcgcaga
     8701 cttgtccacc cgacgggcca gtgctcaccg ctccggcgga cggcgccacg aatgtcgact
     8761 cccccgtccg cttcgattgg aaccccgtca gcagtgccat cggatacgcc gtcttcgtcc
     8821 gccacaacga cggcgcgccg acggagctgg ccgatacaac cacgcgtacg gcagtgacca
     8881 aggccatccc ggagggccaa acggagtggt gggtggttgc gttcttcaac ggctgcccgc
     8941 ctgccgagtc gaagcacgcg attttcacaa tcccggaaac gaactgcgac aacccgcgcc
     9001 cgatcctctt cacgccggcc gacaacagca cgggcctggc atcacccgtt cacttcgagt
     9061 ggtcccgcat cgacaacgcc acgaagtaca aagtctgggc tgcggtcgat gacgaagacg
     9121 ccagcgtcat cggcacgacg acggtcaaca agttgacggt cggcgtgccc agcggcacga
     9181 tccactggta cgtcgaagca ttcttcgact cctgcccgtc tctcgactcg gccaccggca
     9241 agttcacggt gcgtaaatcg gcgccgccct gtgacacgcc ggaccggccc aaagctgccg
     9301 cccccgcgca agtcgcttcg aacagcagct acaccgttca ctggagtgcc gtgctgaatg
     9361 cgacgagtta cgagttgcag gagtcgtcga ccgcggactt tgcggcggcc accacgcaag
     9421 tcgtgaccgg cctctccgcc agcttcgtgc acacggcagc agccaatccg cagaagtggc
     9481 gctaccgcgt acgcgccatc agcagttgcg acgacgagcg cgggccgtac tcacgcatcg
     9541 tgattgtcac cgtgcttccg gacgcgccgc agaagcagac cagcgtcgag atcggtacgc
     9601 agagcacgat ccgccagacc atcttcatcc cgggccatac gcctccattg ccattcacgg
     9661 cccgcacgga caaaccgtgg gcctcggtga cgccttcatc cggaacgctc ggtacccagg
     9721 gcgtgacgct gacgatcgtc tccgatccaa cggcgctgaa actcggcacg aacaccgcga
     9781 ctgtgatcct cacctttagc gcagcaacca agggcggcgt cagcgccagc gacaccacga
     9841 cgccgcccgg cagcgtgccg gtctcggtca cgaccgtgac gccggtctcc tccggcggca
     9901 agagcggacc gctccccaac tcgctcatca tccccgcagt cggtcacgca accggcgcca
     9961 acagctcgct cttcgagtca gatctccgca tcgccaacat cacagcccag gcgatgaaat
    10021 accagctcaa tttcacactg acggcaacgg acggcacgca gtccggtcag acgacgacga
    10081 tccaggtcga tcccggctcg accatggcac tcgacgacat cctgaccagt ttcttcggcg
    10141 acggttccga cggatcgagc gccctaggcg tgctggagat ccgcccgctg acgacgacca
    10201 cgcagagcgg cgcaccgagc gtgcagaccg tcgcttccag ccgtacctac aactcgacgc
    10261 cgaccggtac cttcgggcag ttcattccag ccattccgtt ctcccagttc atcgctcagt
    10321 cgcaagattc gtcgccgaac ccgatcatca cgctgcagca gatcgcgcag tcagccgcct
    10381 atcggacgaa cttcggcatc gtcgagggag cgggagagcc ggccaacgtc ctcgtccacg
    10441 tcttcaacaa ccagggcatc gaagtcgtgg cgccgattcc gttgtcgctc ctgcctggcg
    10501 agcaccagca gcgaaaccta ctcgcggaga atggcatcac gctcaccgat ggccgtttcg
    10561 aggtcgaagt cgtgtcggcg accggcaagg taacagcgta cgcatccgtc atcgacaatc
    10621 ttacgaacga cccgctgctg gttttcccag tactgaaggg cgccgagtcg gccacgcgtt
    10681 acgtcatccc gggcgtggcc gacatcaaca acggcttcgc cagttggcgc agcgacatcc
    10741 gccttttcaa tcccggctct accccggtca cggcgacgct gacctatttc ccccagcccg
    10801 gcaacaccgg agcgagcgga ccgaaacagg tgaccatccc cgcgggccag gtgatgccga
    10861 tcgacaacgc gctgcagaat ctcttcacgc tgaccaactc cggcggctcc atgctcgtca
    10921 ccacgccgtc gagctcgaag ctcgtcgtca ccgcgcgcac atacaaccag acaacgaacg
    10981 gcacctacgg ccagttcatc cccgcggtaa cgccggccgg atccgtcggc ctgtccgaca
    11041 accgtgtcct gcaactcctc caactcgagt catccgacaa gatgcgcacg aacctcggct
    11101 tcgtcgaaac gagcggcaac ccggtcacga tcgaagtgtc ggccattccg tccgattcga
    11161 aggtcgccgc gaagacccag atcccactcg ccgcaaacca gttcctgcaa gtctccctcg
    11221 cccaattcgg cctcggcacc gcctacaacg cccgagtcac ggtgaaggta ctcagcggca
    11281 ccggccgcgt cacggcatac ggatcggtga tcgacgccgc gacgcaggat ccgacgtacg
    11341 tgccggcgca gtaggtttta gcggg
//
LOCUS       DATLVW010000097         4525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_336844, whole genome shotgun sequence.
ACCESSION   DATLVW010000097 DATLVW010000000
VERSION     DATLVW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336844"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VNN25_03275"
     CDS             <1..200
                     /locus_tag="VNN25_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HXH90577.1"
                     /translation="RLTRDIESRGRSLKSVIDQYLTTVRPMHEEFVEPSKRHADLIIP
                     EGGENQVALDAIISRVEHLLV"
     gene            394..600
                     /gene="rpsU"
                     /locus_tag="VNN25_03280"
     CDS             394..600
                     /gene="rpsU"
                     /locus_tag="VNN25_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXH90578.1"
                     /translation="MPLVHVKEDESFENALRRFKRKCEKSGILSELKKRQHYEKPSMK
                     RKRKAIAARKKVLRKLAEERRTGY"
     gene            843..3209
                     /locus_tag="VNN25_03285"
     CDS             843..3209
                     /locus_tag="VNN25_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXH90579.1"
                     /translation="MSPFPLQQLELDRVLTLIAMEAKSSLGKEGISRRRPHRTLAACE
                     AAQADLAEMVRFFLTDGLLPLAGLTDVALLLDRDSILDIDESWIVVRCVRATQAIRET
                     FLRTDTFPRMAAHAQGIPDLGSLISKLNKYFTQDGKLREEASAELRSIRQRMQGKRSA
                     IQRVLNDVMNRSADAIQEPLIVMRGDRYCIPVRADHRNAVTGILHERSGSGASFFIEP
                     MQAIELNNDLADLLIQEREEIARITRFISAAIAEEADLIRDAASVGGEFDALQACAQF
                     HQIVGASRPAFNDDHVLEMIDGRHPLLDERLASARAEAFGEEPSDRRVMPISVRLDQS
                     STALVISGPNAGGKTVALKTTGLLVAMAMSGLPVPAADGTIIPIVDDFHVLIGDDQSV
                     LEHLSTFSAYLVRLRRVLENATARSLVLLDELGSGTDPEEGSAIATAVIEHLLATGAL
                     MIVTTHLSALKSFAVNDTRIINASMEFDSATAAPTYRMIAGIPGRSRAIDVAQIIGLP
                     SSVIDRARQHLGDRYGETDTLIAELQKKMSEVLSERDEAAGLKTSLENEIGRLALERG
                     RLEKERAKLGGSFREELDRLRDDVQRQVAAELKNLRESDRNSRASANAAEIVKTLTKP
                     VERALDFLPVEQREVHVGEKAEHRSFKVTGTIVSIEGKKAVLNVNGRKMTVEVKDLVP
                     RESGAGGRESKKTGVRPASPDSLVPEVSAELNLIGQRVDDAIDESDKFLDRSLMEGKQ
                     AVRIIHGFGTGALRKALRDHLRKHPAVKSWRPGGENEGGDGATIAVLE"
     gene            3271..>4525
                     /gene="dnaG"
                     /locus_tag="VNN25_03290"
     CDS             3271..>4525
                     /gene="dnaG"
                     /locus_tag="VNN25_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXH90580.1"
                     /translation="MAFSDLNDSVIAQVRQAADIVDFIGQVTPLKLAGKSYKGLCPFH
                     REKTPSFTVDRGKGLFYCFGCGTGGDVFKFLTLTERFTFPEAVEHVASRVGIELPKKK
                     RTARESDKDDLLEVMDEASEAFHQALGWGDNPAKRYLTERAVAQEIVDKYGFGYAPDS
                     WDYILTRLARKHGEKKLETAGLAMPQKTGNGYYDRFRNRLMIPIHNESGTVVGFGGRS
                     LDGSDPKYLNSPESEIFNKSRLLYNLHRSKDAMRRIDRAILVEGYFDAISIDHAGVPG
                     VVASMGTSLTTGQASMMRRFTTRVVIAYDGDNAGRMATLRAAPVLLAAGLSVSALDLQ
                     GEKDPDSVVQKHGVDRFLEILGSATDIFEFALAEWGTDVAKLTGREKSERVEQFVPLL
                     SAVSDPVVRNDAAQRIADAFRLEFQT"
BASE COUNT          937 a         1422 c         1363 g          803 t
ORIGIN      
        1 ggcgactcac gcgcgacatc gaatcgcggg gtcgaagcct gaagtccgtg atcgatcagt
       61 acctgacgac cgtccgtccg atgcatgagg agttcgtcga gccgtccaag cgtcatgccg
      121 acctcatcat cccggaaggg ggcgagaacc aggttgcgct cgatgccatc atttcgaggg
      181 tcgaacacct tctcgtatag ttattcgcgt tcccgtcgac cgcaaacgac ggccttgcag
      241 cagcttacaa aattaacagg atgccggaat ggagaggcgc tctccggccg tttcggtgag
      301 ctgccgtagc cgcattgaaa gcattgcccg ctgccacggt gctcttttag aatcacatcc
      361 acgggaccgc tacgcagaag ggggggattg ggattgcctc tggtgcacgt caaagaggac
      421 gagagctttg aaaacgcgct tcgccggttc aaacggaaat gcgaaaaatc ggggatcctc
      481 tccgagttga agaaacggca gcattacgaa aagccgagca tgaagcgtaa gagaaaagcg
      541 atcgctgcgc ggaagaaagt gttgcggaaa ctcgccgaag aacgtcgtac aggttactga
      601 gatccatcca atcaccaaaa cgaaggaccc gcggaaacgc gggtcctttt ttttgcgctt
      661 ctcagagatc aaagactttc tcacgcgaag acgcgaaggt ttgatgtttg cgctgctctt
      721 cttcgcgacc ttcgcggctt cgcgtgagaa atctctgagt ctctgtggtt catcgttctc
      781 ctcggccgag cgccacaaaa cggaatagcg gaccaaagca tcacgtctaa gcaaccgtta
      841 ccgtgagccc cttccccctc cagcaactcg agctcgaccg cgtcctcacg ctcatcgcga
      901 tggaggcgaa gagctcgctc ggcaaagaag gcatttcccg ccgccggccg catcgaacgc
      961 tcgccgcgtg cgaagcggcg caagccgatc tcgccgagat ggtgcgcttc ttcctcacgg
     1021 acggactgct tccactggcc gggctgaccg atgtcgctct cctgctcgat cgcgactcga
     1081 tcctcgacat cgacgaatcg tggattgtcg tccgctgcgt gcgcgcgacc caggcgattc
     1141 gcgagacgtt cctccgcact gacacgttcc cgcgcatggc tgcccatgcc caaggcatcc
     1201 ccgacctcgg ctcactgatc tcgaagctga acaagtactt cacgcaggac ggcaagttgc
     1261 gcgaggaagc gtcggcggag ctgcgctcga ttcgtcagcg catgcagggc aaacgctcgg
     1321 caatccagcg cgtcctgaac gacgtgatga atcgcagcgc cgacgccatc caggagccgc
     1381 tcatcgtcat gcgcggcgac cgatactgca tccccgtccg cgccgatcac cgcaacgccg
     1441 tgaccggcat cctgcacgag cgctccggct ccggcgcgtc gttcttcatc gagccgatgc
     1501 aagctatcga gttgaacaac gatctggccg atctgctcat ccaggagcgc gaggagatcg
     1561 cccgcattac gcgcttcatc tcggctgcca ttgccgagga ggccgatctc attcgagatg
     1621 cagcgagcgt cggcggtgag ttcgatgctc tgcaggcatg cgcgcagttt catcaaatcg
     1681 tcggcgcatc gcggccggca ttcaacgacg atcacgttct cgaaatgatc gacggacgtc
     1741 atccgctgct cgacgagcgc ctcgcctccg cgcgcgcgga agccttcggc gaagagccct
     1801 cggatcgcag agtcatgccg atctccgtcc gcctcgatca gagcagcacc gcactcgtca
     1861 tcagcggacc gaatgcaggc ggcaagacgg tggcgctcaa aacgaccggg ctactcgtgg
     1921 ccatggccat gagcgggcta ccggttcctg ccgcggacgg aaccatcatc ccgatcgtcg
     1981 acgactttca tgtcctcatc ggcgacgatc agagtgtcct cgaacatctc tcgacgttct
     2041 ccgcgtacct cgtgcggctg cgccgcgtcc tcgagaatgc cacggcgcgc tcgctcgtat
     2101 tgctcgacga gcttggctcg ggtaccgatc ccgaggaagg atcggcgatc gcgacggccg
     2161 tcatcgagca cttgctcgcc accggcgcgc tcatgatcgt gacgacgcat ctatccgcgt
     2221 tgaagagttt cgcggttaat gacacacgca tcatcaacgc ctcgatggag ttcgactccg
     2281 caacagcggc gccgacctac cggatgatcg ccggaatccc cggccgtagc cgcgcgatcg
     2341 atgtcgcgca gatcatcggt ctcccctcct ctgtgatcga ccgcgcccgc cagcacctcg
     2401 gcgaccgata cggcgagacc gacacgctga tcgccgagct gcagaagaaa atgtccgagg
     2461 tactctccga gcgagacgaa gccgctggct tgaagacctc gctcgagaat gagatcggac
     2521 ggctggctct ggagcgcggt cgattggaga aagagcgtgc aaaactcggc ggctcattcc
     2581 gcgaggagct cgaccgattg cgtgacgacg tgcaacgcca ggtggccgcg gagttgaaga
     2641 acctccgcga atcggaccgc aactcgcgtg cgagtgcgaa cgccgccgag atcgtcaaga
     2701 cattgaccaa gcccgtcgag cgcgcgctcg actttcttcc cgtcgaacaa cgcgaagtac
     2761 acgtcggcga aaaagcagag caccgctcgt tcaaagtcac cgggacaatc gtctcgatcg
     2821 agggcaagaa agccgttctg aatgtgaacg gacggaagat gacggtcgag gtgaaggatc
     2881 tggttccgcg ggagtcggga gccgggggtc gggaatcgaa gaaaaccggc gtacggcccg
     2941 cgagccccga ctccctcgtc ccggaagtct ccgcggagct gaacctcatc ggccagcgcg
     3001 tcgacgacgc catcgacgag tcggacaagt tcctcgaccg ctcgctcatg gaaggaaaac
     3061 aggccgtccg catcattcac ggctttggaa ccggcgcgct ccgaaaggcg ctgcgcgatc
     3121 acctgcgcaa acatcccgcg gtgaaatcgt ggcgtccggg cggtgagaac gaagggggcg
     3181 acggggctac gattgcggtg ctggagtgat ttcaagcaat cggaccgatc ggtcggatcc
     3241 gtccgatccg tccgattgaa ctaacaaacc atggccttct ccgacctcaa cgactccgtt
     3301 atcgcgcagg tgcgccaggc agccgacatc gtcgacttca tcgggcaggt caccccactc
     3361 aagctcgccg gcaagagcta caaaggcctc tgcccgtttc atcgcgagaa gacgccgtcg
     3421 tttaccgtcg accgaggcaa aggtcttttc tattgcttcg gatgcggcac cggcggagac
     3481 gtcttcaagt tcctcacgct gaccgagcga ttcacgttcc ccgaggcggt ggagcatgtc
     3541 gcgtcgcggg tgggaatcga gttgcccaag aagaagcgaa cggcgcgcga aagcgacaaa
     3601 gatgacctgc tcgaagtaat ggatgaggcc tccgaggcct ttcatcaggc gcttggatgg
     3661 ggcgacaacc cggccaagcg ctacctcacc gagcgcgccg tcgcccagga gatcgtcgac
     3721 aaatacggtt tcggctacgc gcctgattca tgggattaca tcctgacgcg gctggcgcgg
     3781 aagcatggcg agaagaagct tgagaccgcc ggactggcaa tgccgcagaa gaccggcaac
     3841 ggctactacg accgtttccg caaccggctg atgattccga ttcacaacga gagcggaacc
     3901 gtcgtcggat tcggcgggcg atcgctcgac ggaagcgacc caaagtatct caactcgcca
     3961 gagtcggaga tcttcaacaa gtcgaggctc ttgtataact tgcaccgatc gaaggatgcg
     4021 atgcgccgca tcgaccgggc catcctcgtc gaagggtatt tcgacgccat ctcgatcgat
     4081 cacgccggtg tgccgggcgt ggtcgcgtcg atgggaacgt cgctcacgac gggacaggcg
     4141 tcgatgatgc ggcggttcac gacgcgcgtc gtgatcgcct acgacgggga caacgccggc
     4201 cgcatggcca cgcttcgggc tgcgccagtg cttctcgctg ccggactgag cgtctcggcc
     4261 ctcgacctcc aaggcgaaaa ggaccccgat tcggtcgtcc agaaacatgg cgtcgaccgt
     4321 tttctggaga ttctcggcag cgccaccgac attttcgagt tcgccctcgc cgaatggggc
     4381 acggacgtcg ccaagctcac cggacgcgag aaatcggagc gggttgagca attcgtgccg
     4441 cttttgtccg cggtttccga tcccgtggtc cgaaatgacg cggcgcaacg gatcgcggac
     4501 gccttccggc tggagttcca gacga
//
LOCUS       DATLVW010000098         3551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_582378, whole genome shotgun sequence.
ACCESSION   DATLVW010000098 DATLVW010000000
VERSION     DATLVW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582378"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VNN25_03295"
     CDS             <1..233
                     /locus_tag="VNN25_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH90581.1"
                     /translation="RPFTPGDTLVLYTDGVTETANRDAEIWGDERLRTTITNHVNGAS
                     LATTIISDVAQWRGTNGPPDDDVTLIVVRRKN"
     gene            300..542
                     /locus_tag="VNN25_03300"
     CDS             300..542
                     /locus_tag="VNN25_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90582.1"
                     /translation="MRNRFVGVLLVTALSLWSAAGFGQEKKKEAEPLKIKVGDVAPDF
                     TLVEYDGQKVKPVSLHDFKGKKQVALAFFVFAFTGG"
     gene            552..884
                     /locus_tag="VNN25_03305"
     CDS             552..884
                     /locus_tag="VNN25_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXH90583.1"
                     /translation="MKAFQASMAKMEGSDTQVLGVSIDSPFANHAFAQQNGITFPILG
                     DMDAKAIKAYGLEKEYHIGGATMASARRATFLIDKEGKITAEQVDSEAVDPTKIVDAC
                     QISQHKTK"
     gene            complement(890..1402)
                     /locus_tag="VNN25_03310"
     CDS             complement(890..1402)
                     /locus_tag="VNN25_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HXH90584.1"
                     /translation="MTSVDLLTSSAVTIASIPQSFLDRVRSEGIDDLGQPVKRLVAAG
                     GEPCRDVLRRATAGEELILASFTPFGKPGPYKEYGPVFVLAHAGDESVRRDALPPAGS
                     ATDYLREQFVIRAYSTGEEIVDAVMVSAPALEETVDRFFQSPGTAFLHVRFPTYGCFA
                     MRIDRAHGEA"
     gene            1926..2810
                     /locus_tag="VNN25_03315"
     CDS             1926..2810
                     /locus_tag="VNN25_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH90585.1"
                     /translation="MNPRIALFIASTCALLVACASSHRNPAVTPETFVLDYNTPTDRR
                     LQARIEAIDTRLRAKYGMSTEQTAVGVLDLNRLRLAMIHPDRIEYAASVAKVGILLAY
                     FQLHPDAATQMDAETQHELGLMIKASSNEMAAKYSRAMGLREIQQVLNAQGFYDAAHG
                     GGIWVGKHYGPNSERIGDPVANHSHAATVRQLLRFYWLLSRDQLVSPAASKKMREIFL
                     SPDIPPDDHKFVKALAGRGLTILRKSGTYQDWLHDSAIIEGPNRRYVLVGLTHHPKGD
                     DYLEDLAREVDDLMARRW"
     gene            2947..>3551
                     /locus_tag="VNN25_03320"
     CDS             2947..>3551
                     /locus_tag="VNN25_03320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH90586.1"
                     /translation="MRVSIVTPSLQQGQFIERTLASVWRQNCGGLAGEIEHIVMDGGS
                     TDGTVEILERWQDRISFSTGPDGGQSAAINAGLAMAGGDILAYLNSDDVYCDGAVAAV
                     VAAFEADPSVDVVYGDADFVDCDDRIIARYPTEEWSPERLKLVCFLCQPAVFFRRRVL
                     EQFGPFNANLRYCMDYEYWLRLAARGVRFQHIPVTVAASRI"
BASE COUNT          714 a         1067 c         1107 g          663 t
ORIGIN      
        1 cccgcccctt cactcccggc gatactctcg tcctctacac cgacggcgtg accgaaaccg
       61 cgaaccgcga cgccgagatc tggggcgacg agcgcttgcg aacaacgatt accaaccacg
      121 tcaacggcgc gtcgctggct acgaccatca tcagtgatgt ggcgcagtgg cgaggcacca
      181 atggcccgcc agatgacgac gtgacgttga tcgtggtcag gcgaaagaac taagttcaac
      241 ttgatggcag gcatacacgt ttgagttaat ctaatcctgc atattcgagg aggtgccgca
      301 tgcgaaatcg tttcgtggga gtccttctgg tgaccgcgct cagtttgtgg agcgcggcgg
      361 gcttcggcca ggagaagaaa aaagaggcgg agccgctcaa gattaaggtt ggggacgtcg
      421 cgcccgactt taccctcgtc gagtacgacg gtcagaaggt caagcccgtt tcgctgcacg
      481 acttcaaggg caagaagcag gtggcgcttg cgttcttcgt cttcgcgttc accggtggct
      541 gaacgcagga aatgaaagcc ttccaggcca gcatggccaa aatggaaggc tcagacaccc
      601 aggtcctggg tgtgagcatt gacagcccct tcgcaaacca cgctttcgcc cagcagaacg
      661 gcatcacctt cccgatcctc ggcgacatgg acgccaaagc gatcaaggcg tacggcctcg
      721 aaaaggagta ccacatcggc ggcgcgacga tggcgtcggc gcgccgggcc acgttcctga
      781 tcgacaagga agggaaaatc accgcggaac aggtcgacag cgaggcggtc gatccgacga
      841 agatcgtcga cgcctgtcag atcagccagc ataaaacgaa atagctccct cacgcttcgc
      901 catgcgcgcg atcgatgcgc atggcgaagc agccatacgt cggaaagcgg acgtgcagga
      961 acgccgtgcc gggcgactgg aagaagcgat cgactgtctc ttcgagcgcc ggcgcgctga
     1021 ccatcaccgc atcgacgatc tcctccccgg tcgaatacgc gcgaatgacg aactgctcgc
     1081 gcagatagtc cgtcgcggaa ccggccggcg gaagggcatc gcgccggacg gattcgtcgc
     1141 ccgcgtgagc cagtacgaaa accggaccat attccttgta aggccccggc ttgccgaacg
     1201 gcgtgaaact cgcgaggatc agttcctccc ctgccgttgc ccggcgcaga acgtcccggc
     1261 acggctctcc gcccgccgca acgagacgct tgaccggctg cccgaggtcg tcgatgcctt
     1321 cggagcggac gcggtccaga aacgattgcg gaatcgaagc gatcgttacc gcggaggatg
     1381 tcagcagatc gacggatgtc atgtgttctc cctcttttct agagagaaca cggggatacg
     1441 gcgcggcgtt cgcggtcggt gccaaaaact cttaggcaga gttagggttg agtgtcatcc
     1501 ggagtgtgat ccggagagat catccaatcg agattggtgt catccggagt gtgatccgga
     1561 gagatcatcc aatcgagatt ggtgtcatcc tgagactgcc ggagccgccc gcgcgaatcg
     1621 cgatatttgc gcagtacgtg ctcggcggcg taggacgtcg aaggatctca aacttcgcag
     1681 atcatgtttg tccagcgcga gcgaacgtga tcaaagacgc tcgaacaaga tcctcgcatt
     1741 ttgagatcct tcgacgtcct ccgccgcaat gcctgccggt tgcccgagta gcgtaaggcg
     1801 cggctccggc gactcaggat gacacatcac ggtttgagcg catcctcaac ggcaagtatg
     1861 agaaatcaga ccggcgccag ttccttggtt accttcttcg agtcagcgca gttatccttc
     1921 ggcccgtgaa tcctcgaatt gctctcttca tcgcaagcac ttgcgcgctt ctggtcgcat
     1981 gcgcatcgag ccatcgaaac ccggccgtca caccagagac cttcgtgctc gattacaaca
     2041 cgcctaccga tcgccgtttg caggcacgca tcgaagcgat cgacacgcgg ttgcgcgcga
     2101 agtacggcat gtcgacggag caaaccgccg tcggcgtgct cgatttgaat cggctgcggc
     2161 tggccatgat tcatcccgat cggatcgagt acgcggccag cgtggcgaag gtcggcatcc
     2221 tgctggcgta ttttcaactg catcctgatg ccgctacgca gatggatgcg gagacgcagc
     2281 atgagctggg gctgatgatc aaggcttcca gcaacgagat ggcggcgaag tactcgcggg
     2341 cgatggggtt gcgcgagatt cagcaggtgc tcaatgcgca gggcttctat gacgcggcgc
     2401 atggcggcgg catctgggtg ggcaagcatt acgggccgaa cagcgagcgc atcggcgacc
     2461 cagtcgccaa ccactcccat gccgcgacag tgcgccagct tttgcggttc tactggctgc
     2521 tgtcgcgcga tcagctcgtt tcgcctgccg cgtcgaagaa gatgcgagag atttttctct
     2581 cacccgatat cccgcccgac gaccacaagt tcgtgaaagc gcttgcgggc cgaggactca
     2641 cgatcctccg caagtcgggc acgtatcagg attggctgca cgactccgcc atcatcgaag
     2701 gtccgaaccg ccggtacgtg ctcgtcgggc tgacgcatca tccgaagggc gatgactacc
     2761 tcgaggatct ggcgcgcgaa gtcgatgatt tgatggcgcg gcggtggtga aggagcctgc
     2821 cgggatggcg taacttcggc ggcatgctcc cgagtagaag cacccgggcg ggacgccctg
     2881 gtgcccgcgg acgagacgtc cgcactcctt gctatccgca agggcctgcg gaacaatcga
     2941 tttcgcatgc gcgtgtccat cgtcacgccc tccctgcagc agggccaatt catcgagcgt
     3001 acgctcgcga gcgtctggcg gcagaactgc ggcgggctcg ccggtgaaat cgaacatatc
     3061 gtcatggacg gaggcagcac cgacggcacg gtggagattc tggagcgatg gcaggaccgg
     3121 atctcctttt cgaccggtcc ggacggcggg cagtcggccg caatcaatgc cggactggca
     3181 atggccggcg gcgacatcct cgcctacctc aactcggacg acgtctactg cgatggtgcc
     3241 gttgctgccg tggtcgcagc gttcgaggcc gatccttctg tggacgtcgt ttatggcgac
     3301 gccgacttcg tcgactgtga cgatcgcatc atcgcccgct atccgaccga ggagtggtcg
     3361 ccggagcggc tgaaactggt ttgctttctc tgtcagccgg cggtgttttt ccggcgcaga
     3421 gtgctggagc agttcggacc gttcaatgcg aatctccgtt actgtatgga ctacgagtat
     3481 tggctgcggc tggccgcgcg aggggtgcgc ttccagcaca tcccagtcac ggtggcggct
     3541 tcgcggatcc a
//
LOCUS       DATLVW010000099        17717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_293130, whole genome shotgun sequence.
ACCESSION   DATLVW010000099 DATLVW010000000
VERSION     DATLVW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17717
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293130"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VNN25_03325"
     CDS             <1..441
                     /locus_tag="VNN25_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH90587.1"
                     /translation="RGLRGGADDYITKPFGLQELFARIDAVMRRSDATSVTTKTVCED
                     TLIKLDPSTREASAKDQKLKLSPKEFDLLMYLFRNKDRVVPRDELLSRVWNYRVDLNL
                     NSRTVDTHVTRLRRKLQKAVGTDAAVIVAVAKIGYRYLGEATAA"
     gene            607..1593
                     /gene="cysK"
                     /locus_tag="VNN25_03330"
     CDS             607..1593
                     /gene="cysK"
                     /locus_tag="VNN25_03330"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXH90588.1"
                     /translation="MHGKIYDNITELIGRTPLVRLNRVVPAGAAEVAAKLEAFNPLSS
                     VKDRIAISMVTEAEKAGEIKPGNTIVEATSGNTGIGLAFVAAARGYKCTLVMPDTMSV
                     ERRKLLQALGAELILTPGTAGLPEALKIAREYAEKTPGAWQSKQFDNPANPKVHRETT
                     AREILDDTGGKVDAIVAGVGTGGTVSGVGQVLKKHNPSILIVAVMPVEAQTLKGGPYA
                     PHKIQGIGPSFVPAIYDKDVVDRVLDVTYDQAVDTARRLAKQEGILVGISSGAIAWAA
                     IEVAKELGAGKRVVVVLPDTGERYLSTPLFEYTDLVNQPATTPQPAAAPTVK"
     gene            1599..2072
                     /locus_tag="VNN25_03335"
     CDS             1599..2072
                     /locus_tag="VNN25_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXH90589.1"
                     /translation="MKRFLVIAILIATPALASDITRASVIAAMNDYRAQHHLPLLRED
                     VRLDAAANDRMRDMEDQGYWAHESPDGRSPFLWLPAHGYEIRYAGENLACGFETTEML
                     VEGWMESPGHRDNILSPHFEDCGIAIIDGMTMRRGNGKSIVVMFGTARTPAMAAR"
     gene            2628..3833
                     /locus_tag="VNN25_03340"
     CDS             2628..3833
                     /locus_tag="VNN25_03340"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXH90590.1"
                     /translation="MKRITVAWLSLVTAVSLSAQMRTNAARPAPPAPVTPAAGDALAG
                     LTAAQRSAFADGLADFKSVEEVADGLGPVFNERSCAACHTTPAVGGGSTRTVTRFSRR
                     VNGVFDPLASLGGSLMQDHALTLADGVSHAFTPESVPAIATIVVQRRTTPLFGLGLVD
                     ATPDADFLALAQMQASRGDGVVGRASVVDNIRAGMKTVGKFGWKAQVPTLFQFSGDAY
                     LNEMGITTPDFPNENCPQGNCAELSFNPAPGLNDDGSGPVLLTNFMTLLAAPPRAAET
                     RDTSDGEATFDRIGCTACHVATMHTGANAIAALDRKTYHPYSDFLLHDMGSLGDDLEM
                     GSATGREMRTTPLWGLRFVTRYLHDGRATTLDQAILAHEGQGSGARDRYAALPAAVKS
                     KVIAFLQSL"
     gene            complement(3875..3997)
                     /locus_tag="VNN25_03345"
     CDS             complement(3875..3997)
                     /locus_tag="VNN25_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90591.1"
                     /translation="MNSKRNSLLIIVTVVAVLILVVWLGGGAMWHMLLKMHGRG"
     gene            complement(3990..6419)
                     /locus_tag="VNN25_03350"
     CDS             complement(3990..6419)
                     /locus_tag="VNN25_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXH90592.1"
                     /translation="MTTRWKKAVRDFWQESTRTVLVVLAIAVGISGFAAVLASYAILT
                     RELNRGYLATNPASATLRTDAVDEGLVAAIAATPGVAEVEARRTVSGRIRGASGWRNL
                     TLFVVKDFGRIRVSNLNPEQGAWPPATGEILIERDAFQVARIHIGDAVTVRTTNGPER
                     TLRVSGGVHDVGQAQARMENVVYGYITQATLGQLGEEPVLNQIKFVVSGDAFDLPHIE
                     AVTADVKRTIERRGHPVTRVDIPAPGKHPHADIMGLLLLSLSSFGLFVLILSGVIVVN
                     LLSALMAAQIRQIGIMKAIGGTRWQIARIYFGQALLLGIAAIVIAVPVGIAGSRALCR
                     AMAVFLNFDITSFAIPIWVYALVAAVGLLVPLLAAAFPVWRGSGVSVREALADFGVTG
                     SSFGTSAIDRMLGGVGGLTRPLLLAIRNNFRRRARLALTVLTLAAGGLFFMTALNLRA
                     SMINTLDRLFATKTYDLGVSLAGMAPAGDVLRIARSTPGVRSAECWIASEGVLPDAAQ
                     VPPHTSGVTHGGITAGAVSDNRFIVIALPSESQMVKPNLESGRWLRPGENDTIVVNSA
                     LAAKSAQMRVGNRVTFPMGAGPVSLQVVGIVAEPFSPPMAYMPRAFLDEKAAHAGVTN
                     NVRLILDKTDQASIDRVKALLEDGFAQAGIKAVSMTSKNDNRFGFDQHMLMIYVALII
                     MSSMIGGVGGLGLMTTMSLNLLERRREMGVMRAIGATPHAILLMLVAEGSIVGFASWA
                     LAALAAWPLGKGLGDYLVSKLFKSGLDFTFELRGLAVWLAVSLILGAIASFVPAWDAT
                     RHPVREALTYE"
     gene            complement(6416..7123)
                     /locus_tag="VNN25_03355"
     CDS             complement(6416..7123)
                     /locus_tag="VNN25_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH90593.1"
                     /translation="MINLRNVSKTYETTAGGFTALRNLDLDVLRGELVAVVGKSGSGK
                     STLLNVIGGIDAVSTGTVTIADASIQSLGESRLAAWRGRTVGFVFQFFQLLPTLTAAE
                     NVMLPMDFCGTFPPRERRRRAMTLLDRFGVSAQADKLPASLSGGEQQRVAIARALAND
                     PPIVLADEPTGNLDSANAGAVLDLFRAMASSGTTVLIATHDRDVARVIDRTIELADGV
                     LLPAAPAPLAVAAGTGA"
     gene            7308..7697
                     /locus_tag="VNN25_03360"
     CDS             7308..7697
                     /locus_tag="VNN25_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH90594.1"
                     /translation="MNQSRCAVLIVEDDDKLQELLRTLISRHCTSVDLAADGEQGIEM
                     LRAGTYDAVIVDLMLPKKNGLLVIDAIASLSYSPRVIVLSAISRYFQDRFPEGTMVLQ
                     KPFEIEKVADLIANLHEPHPELSPSAT"
     gene            complement(7713..10097)
                     /locus_tag="VNN25_03365"
     CDS             complement(7713..10097)
                     /locus_tag="VNN25_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH90595.1"
                     /translation="MTSAADGEEPERESATVSLIPAVLEHAGVGVAVFDAGGRIVVAN
                     AALRQLLGYSNDELLQLTASAITSPEDLQAGTTLFQELLSAKRSRYSIDKRLLHKRDS
                     VVWCRVTATLLHQGADDFAIAIIEDITEEKSIEKASDEVRRAHERYALVARATNDVIW
                     DWDAEQGTIIWNDALRAMFGYEPDQIDNRLQWWDSHLHPDDRERVVSGINSIIEKGGS
                     SWTDEYRFERADGTFTEVLDRGYIARSEDGTPIRMIGAMLDVTERKYAERTAAFMAEA
                     SAVLASSLDYHATVARVAKLMVPLLGDHCFIDLHAERLAAIDGDQLPAPISDPVKEVI
                     ATGKATQLSGSEARATGFRSALIVPLAVSDSQPIGALTLAMSHSGRALSERDLRVVED
                     VARRIALAVEHARLYDDIQKASLAKDEFLAILSHELSTPLTIILGWSGIVSQPDVTGE
                     ELQRGIEAIRGSALAQARLIDDVLDLSRVTTGKLHFQQELVSVGTLVDDALTAVRVAA
                     AAKRIHLVAPDKTPAIVLGDANRLRQVIWNLLTNAIKFTESGGSVALQLASAPSTVRL
                     TVADTGRGIEPDFLAHVFEPFRQADASTTRTHGGLGLGLAIVRYVVEAHGGTVAAFSD
                     GPGHGTRFVVELPAAPRAVVSDDQRHSVADASAGAQRSAELAGVRVLFVDDQSEAREL
                     ANVILSRAGAETTLASSAAEAIATFERVAVDILVTDIAMPEMDGYGLIAEIRRRDALS
                     GRRTPAIAATAYRGADDRQRVLAEGFDAYLAKPMESDTLTAAIASVLAAARAAQ"
     gene            complement(10162..10581)
                     /locus_tag="VNN25_03370"
     CDS             complement(10162..10581)
                     /locus_tag="VNN25_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90596.1"
                     /translation="MSILLVLLLLAFPASTQTTPRADGAKAASPATAKTAVHIVARMW
                     RGRVATSRADEYEKYLYDTGVLKIRMIAGNLGAEMLRRSDGDITEFIVISYWPNREAI
                     KAFAGQDIEKVHFLPRDREFLINPDEFVRHYDATSAP"
     gene            10659..11813
                     /locus_tag="VNN25_03375"
     CDS             10659..11813
                     /locus_tag="VNN25_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXH90597.1"
                     /translation="MKTPSNERQRALQILRRIERESAYASLILQNDSGFVRTLVLGVL
                     RWRSRLDFVIDAFAKGDIEPEIRDILRIGALQLLFMNVASHAAVSETVSLVPLRARGF
                     VNAILRRIASKPIPEPPDVATRTAHPRWLIERWTKTFGAERAAKIAAANQELSFPDVI
                     TDAPPSGATESELVPGMWKLTGSSADVDGIVLDEGSAVIAGIAAALGDDVLDLAAAPG
                     GKSRVMRTRGAHVVSNDVSISRLRPLIGKSASIVVSDGRQPPFARRFRVVLLDAPCSA
                     TGTIRKNPEIKWRLQEADLIAFASLQRELLASALDLASESVVYATCSLEPEENDDVVG
                     ELLRNRTDFVRGDVADLVNENVARWVENGVLRLTPEAGTDGFTAHWLRRT"
     gene            12018..12314
                     /locus_tag="VNN25_03380"
     CDS             12018..12314
                     /locus_tag="VNN25_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXH90598.1"
                     /translation="MAGKGDVINEIAEQAGISKKEAAAAFDAFVKHISDSCQRGDRCA
                     IPGLGSFAVSQRKAREGRNPRTNEKIMIPASKNIRFKAGKDLREAVNSKRKGKK"
     gene            complement(12368..13012)
                     /locus_tag="VNN25_03385"
     CDS             complement(12368..13012)
                     /locus_tag="VNN25_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXH90599.1"
                     /translation="MTNVLTPRAVIFDMDGLLLDSEPLYRVTWQTAAAELGCPIDDDF
                     YSRFVGRGNDEAERLLIERFGDAMPLDEFRVRWKRDFDERLAHAPLPHKPGSVALLDR
                     LDSRGIPKALATSTPRAVALRCLGSLYSRFSVFAFGDEVEHSKPYPDLFLLAASRLGI
                     APDDCLVIEDSEAGVRAARAAAMAVIMVPDLVEPSPEIAAMAVRVCRSLDDVLQ"
     gene            complement(13009..14037)
                     /locus_tag="VNN25_03390"
     CDS             complement(13009..14037)
                     /locus_tag="VNN25_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXH90600.1"
                     /translation="MTTIREMLENIERDTMVAQAARSSETRGRDRDEPEDDLRPSYQR
                     DRDRIIHCKAFRRLKHKTQVFLAPEGDHYRTRLTHVLEVTQIARTIAKSLRLNEVLTE
                     AIGLGHDLGHSAFGHAGEAALNKLVKGGFDHYRQSVRVVEVLENDGRGLNLTIEVRDG
                     ILKHSKGEKGELLRKRAKSRALTLEGDIVRISDIIAYVNHDIDDGIRAGVLTENEIPK
                     EIRKTLGNGAAQRIDRMVRDVIDSTLACGYEAISMSAEALGALEELRTFMFQNMYLTP
                     AVRNEFVKAQRTLTALFEHVTANPGDFLSATSDEPVERLAIDFIAGMTDRYAINLYER
                     LFVPRAFV"
     gene            complement(14186..14689)
                     /locus_tag="VNN25_03395"
     CDS             complement(14186..14689)
                     /locus_tag="VNN25_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH90601.1"
                     /translation="MIAIETANDLQVVRELILEYASSLGVDLSFQDLDHELSTLGDFY
                     ELVLIAHEGDRAAGCVALRRLDDTTCEMKRLYIRPEFRGRNLGRTLAERIIDEARHRG
                     YTRMRLDTLPTMTAAIPLYKSLGFVEIPPYRFNPIEGTKFMELRLRSAGVSPGVSPAA
                     AGRLVRQ"
     gene            complement(14686..15471)
                     /locus_tag="VNN25_03400"
     CDS             complement(14686..15471)
                     /locus_tag="VNN25_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXH90602.1"
                     /translation="MRLLQIDAFTSEPFKGNPAAVCFMDGERDDRWMANVAAEMNLAE
                     TAFLRPREDGWSLRWFTPTVEVDLCGHATLASAHAIWSENISSEAVLRFHTKSGVLTA
                     ARDGDFIELDFPAKRDEQIAAPAGLLDALGTTQATYVGRNQFDYIVELPSEKDVHNLR
                     PDHGVLRQLPVRGIIVTSRADAGEYDFVSRFFAPGSGVDEDPVTGSAHCCLTPYWAQR
                     LGKNEMNAWQASPRGGAVRVRLEGDRVKLAGHAVTVLRGELVV"
     gene            complement(15496..16281)
                     /locus_tag="VNN25_03405"
     CDS             complement(15496..16281)
                     /locus_tag="VNN25_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXH90603.1"
                     /translation="MNPEINNWLQLIFRWGHVVAGVMWIGHLWFFNFVNGPFAGKMDG
                     PTKKLVVPELMPRALFWFRWGAAWTWITGFLLLGLVYYQTKLVLFDQDHVGNPWLWLA
                     VVLITLAVGFVIYNQILKMVKNVTVASIINMVLFAGVYCLLATAGHFSGRALYIHSGG
                     IFGTMMALNVWMVIWPAQQKIIRGIKDGNPADAETVRQAGLRSRQNTYMSVPLIFLMI
                     SNHYPTVYGASMWVRDGILAGVIIVGFLFVRWMYAKAAAVQGF"
     gene            16341..17039
                     /locus_tag="VNN25_03410"
     CDS             16341..17039
                     /locus_tag="VNN25_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH90604.1"
                     /translation="MSPHDFENRVAIVTGGSTGIGRATAEMLAKRGARVAIFARSASA
                     SGQLRENTFAVDGDVSDEAAIERLFAETESRFGDCDVLINCAGMIDPKALIDTVPDDW
                     DRMFAVNVRGTYLATRRALPSMIARRAGSIVNVASISGVIGPEKFPGWVSYCASKAAV
                     ISMTEALAVEVKEFGVRVNSVSPGSVDTKMWAAASGGAPAAMTADEVAEVICFLASDR
                     ARAVNGQDVHVYSS"
     gene            17041..>17717
                     /gene="alr"
                     /locus_tag="VNN25_03415"
     CDS             17041..>17717
                     /gene="alr"
                     /locus_tag="VNN25_03415"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXH90605.1"
                     /translation="MRPTFATIDLERFAHNVDVIARLLPEGARLVPVLKADGYGHGAV
                     ELAKRLTPDRVAMIAVALLEEALELRRASITLPILVLGPISREQISIALDHDVTIGVI
                     GPEELESVCDVARDRDVAIHIKLDSGMGRMGVIESELPRVISMIRAASRLRIDGIYTH
                     FANAEDASDPFTEQQIATYDQMLAVLREHGIDAPRHHFANSPATLNGLVRPSDFARVG
                     IALYGPQR"
BASE COUNT         3373 a         5494 c         5663 g         3187 t
ORIGIN      
        1 cgcggcctgc gcggcggcgc cgacgactac atcacgaagc ctttcggcct tcaggagctt
       61 ttcgcgcgca tcgatgccgt catgcgccgc agcgacgcca cgtccgtcac caccaagacc
      121 gtctgcgaag acacgcttat caagctcgat ccgtccacac gtgaagcgtc ggccaaggat
      181 cagaagctca agctttctcc gaaagagttc gatctgctca tgtatctctt ccgcaacaag
      241 gatcgcgtcg tgcctcgcga tgagctcctc tcgcgcgtct ggaactatcg cgtcgacctc
      301 aatctcaaca gccgtacggt cgacacccac gtgacacgcc tccgccgcaa gctgcagaag
      361 gcggtcggca ccgatgccgc cgtgatcgtc gccgtcgcca agatcgggta tcggtatctg
      421 ggcgaggcga cagcagcgtg attgacaacc aaagccaaac agtcatagcg tcaccgctca
      481 catgaacacg accacttgtt gccgccgcca tacgggaccg ttcgagcgaa cgtaagcgcg
      541 tcaacagcta cttttatcga atgaggcccg ggcgtaccgc tcgggtctct tgttttagga
      601 ggagacatgc acggaaagat ctacgacaac atcacggaat tgatcggacg tacgccgctg
      661 gtgcggctca atcgcgtcgt ccccgccggc gccgcggaag tcgccgccaa gctcgaggca
      721 ttcaatccgc tctcgtcggt caaagaccgg atcgcgatct ccatggtcac cgaggcagaa
      781 aaagccggcg agatcaaacc gggcaatacg atcgtcgagg ccaccagcgg caacacgggg
      841 atcggcctgg ccttcgtggc ggcggcccga ggatacaagt gcactctggt catgcccgac
      901 acgatgtcgg ttgagcgccg caagctcctg caggcgctcg gcgcggaact gatcctgact
      961 cccggcacgg ccggcctccc ggaagcgctg aaaattgcgc gcgagtacgc cgagaagacg
     1021 ccaggggcgt ggcagtcgaa gcagttcgac aatccagcca atccgaaggt ccatcgcgag
     1081 acgacagcac gcgagatcct cgacgacacg ggcggcaagg ttgacgcgat cgtcgccgga
     1141 gtgggtacgg gtggaaccgt aagcggcgtg gggcaggtgc tgaagaagca caacccgtcg
     1201 atcctcatcg tggccgtcat gccggtcgaa gcgcagacgt tgaagggcgg accctacgcg
     1261 ccgcacaaga ttcagggcat cggaccgagt ttcgttccgg cgatctatga caaggatgtc
     1321 gtcgaccgcg tcctcgacgt cacgtacgac caggccgtcg atacggcgcg caggctggcc
     1381 aagcaggaag ggatactggt cggtatctcc tccggcgcca tcgcctgggc agcgatcgaa
     1441 gtcgccaaag agctgggcgc cggcaagcgg gtcgtcgtcg tcctcccgga tacgggcgag
     1501 cgctatcttt cgacgccgct cttcgaatac accgatctgg tcaatcaacc ggcaacaacc
     1561 ccacaaccgg ccgcggcacc tacggtaaaa taactacggt gaaacggttc ctggtcatcg
     1621 ccatcctcat tgcgacaccg gcgctcgcct cggatatcac gcgagcgtcg gtgatcgcag
     1681 cgatgaatga ttaccgcgcg cagcatcatt tgccgcttct gcgcgaggac gtgcgcctcg
     1741 acgccgcggc caatgaccgg atgcgcgata tggaagatca gggctactgg gctcacgagt
     1801 cgcccgatgg ccgttccccg ttcctctggc tgcctgcgca cggctacgag atccgctacg
     1861 cgggagagaa cctcgcctgc gggttcgaga cgaccgagat gctggtggaa ggttggatgg
     1921 aatcgccagg gcatcgcgac aacatcctct cgccgcactt cgaggactgc ggcatcgcca
     1981 tcatcgacgg aatgaccatg cgccgcggca atggaaaatc gatcgtcgtg atgttcggaa
     2041 cggcgcgaac gcccgcgatg gcggcacggt aagcctgcgc taactggcgc taactcgctg
     2101 ttctcacact cttatcgcag accttccaat cgagcgcgaa gccggatgaa ggggcgttcc
     2161 aacgagggca catccgtgca tgtaccgcgt tttgaggcag ttcggacttg gcgtagttga
     2221 tcgccctgcg tgtttgcgcc cccctcatcc gccttcggca ccttctcccc ccgcaaaaag
     2281 cgcgggggga gaaggcgctc gatgagaaag cactccagag aaaaggaaga ggtgtagcaa
     2341 ggagatgaga ggtgtagcag ggagggacac caagtgtgcg agaaatgcgg gcgaaggcgt
     2401 tcgcatctcc cgcctactcc gtggcacgag tcgaattgcc tcgcctctga gtcgccgctc
     2461 tctgaaaccg cgtcgttttg tcccttcttg tgcggctgcc agacgcagcc gtgatggcat
     2521 cgcgattgac taaggtcagt cccaccgaac accagtccca acccccaagc cggcgtatca
     2581 gccggaggtc tgttcacaga gtcacgacga agaaggagag ctctctcatg aaacgaatta
     2641 cggtcgcatg gttgtccctg gtcaccgcag tctcgctcag cgcgcagatg cgcacgaacg
     2701 cggcacgtcc tgcgccgcca gctccggtta cgcctgcggc aggcgacgcc ctcgcaggac
     2761 tgacagcagc gcaacgttcc gcattcgccg acggacttgc cgatttcaaa tcggtggaag
     2821 aagtagccga cggactcgga ccggtcttca acgaacgatc gtgcgcggcc tgccacacaa
     2881 cgccggccgt tggcggcggc agcacgcgca cggtcacgcg cttctcccga cgcgtgaatg
     2941 gagtcttcga cccactcgcg tcactcggcg gttccttgat gcaggatcac gccctcaccc
     3001 ttgccgacgg cgtatcgcat gcgttcaccc ccgagtcggt gcctgccatc gctacgatcg
     3061 tcgtgcaacg ccgcaccact cccctctttg gactcggcct cgtcgacgca acgcctgacg
     3121 ccgacttcct cgcgcttgcg cagatgcagg cgagccgcgg ggacggagtc gttggacgcg
     3181 ccagcgtcgt cgacaacatt cgagcgggaa tgaagaccgt cgggaagttc gggtggaaag
     3241 cgcaggtgcc gacgctgttt cagttctccg gcgacgccta cctgaacgag atgggcatca
     3301 ccactcccga ttttccgaac gagaactgtc cgcagggtaa ttgcgcggag ttgtcgttca
     3361 atccggcgcc cggactcaat gatgacgggt caggtcccgt gttgctgacc aacttcatga
     3421 cgctgctggc cgctccgcca cgcgcggcgg agacccgcga cacgtccgac ggcgaagcga
     3481 cgttcgaccg tatcggctgc acggcgtgcc atgtggcaac gatgcacacc ggcgcgaatg
     3541 ccatcgccgc gctcgaccgc aagacgtatc acccctattc cgatttcctg ctccacgaca
     3601 tgggctcgct cggcgacgat ctggagatgg gcagcgccac gggacgcgag atgcgcacca
     3661 cgccgctgtg gggactccgc ttcgtgaccc gctatctgca cgacggtcgg gccacgactc
     3721 tcgatcaggc cattctcgcg cacgagggac agggtagcgg cgcgcgcgat cgatatgcgg
     3781 cgctgccggc cgcggtcaaa tcgaaagtga tcgcgttcct gcagtcgctt tgaggattgc
     3841 gagcggcggc cggcgcgccg ccgctcgctt cgtttcagcc gcgcccgtgc atcttgagga
     3901 gcatgtgcca catcgcgccg cctccgagcc acacgacgag gatcagcacg gcgacgacgg
     3961 tgacgatgat gagcagcgag tttcgtttgc tattcatagg tgagtgcctc acgaacggga
     4021 tgacgggtgg cgtcccaggc agggacgaag ctggcgatcg cgccaagaat gagtgagacc
     4081 gcgagccaga cggcgaggcc gcgaagctcg aaggtgaagt cgagaccgct cttgaacaac
     4141 ttcgacacca ggtagtcgcc gaggccttta ccgagcggcc aggcggcgag cgcggcgaga
     4201 gcccagctcg caaatccgac gatgcttcct tcagccacca gcattagcag aatggcgtgc
     4261 ggcgtggctc cgatcgcgcg catgacgccc atctcgcggc gtcgctcgag gaggttgagg
     4321 ctcatcgtcg tcatcagccc cagcccgccg acgccgccga tcatgctcga catgatgatc
     4381 agcgccacgt agatcatcag catgtgctga tcgaagccga aacggttgtc gttcttgctg
     4441 gtcatgctga ccgctttgat gccggcttgt gcgaacccgt cttcgaggag cgccttgacg
     4501 cgatcgatcg aggcctgatc cgttttgtcg aggatgagcc ggacgttgtt cgtgacgccg
     4561 gcatgcgctg ctttctcatc gaggaaggca cgcggcatgt aagccatcgg cggagaaaac
     4621 ggctcggcca caatgccgac gacctgcaac gataccggcc cggctcccat cggaaacgtc
     4681 acgcgattgc cgacgcgcat ctgcgcgctc ttcgcggcga gtgccgagtt gacgacgatc
     4741 gtgtcgtttt cgccgggccg cagccaacgt cccgattcga ggttgggctt caccatctgc
     4801 gattccgacg gaagtgcgat gacgatgaaa cgattgtcgg agactgctcc ggcggtgatt
     4861 ccgccgtgtg tgacacccga ggtatgagga ggcacctggg ccgcgtcagg aagaacccct
     4921 tcgctggcga tccaacattc ggcgctgcga acaccgggtg tgctgcgagc gatacgaagc
     4981 acgtcgccag ccggcgccat gccggccagg ctcacgccga ggtcgtatgt cttcgtcgcg
     5041 aacagccggt cgagcgtgtt gatcatcgac gcgcgaagat tgagcgccgt catgaagaat
     5101 aggcctcccg cggcgagcgt cagaacggtc agcgccagcc gcgcgcggcg acgaaagttg
     5161 ttgcgaatag ccagaaggag cgggcgcgtc agaccgccga cgccgccgag catgcgatcg
     5221 attgctgatg ttccgaagct gctgcccgta acgccgaagt cggcgagcgc ttcgcggaca
     5281 gagacgccgc tgccgcgcca aaccgggaac gccgcggcaa gaagcggcac gaggaggccg
     5341 accgcggcga ccagcgcgta gacccagatc ggaatcgcga aactagtgat gtcgaagttg
     5401 aggaagaccg ccatcgcgcg gcagagcgcg cggcttccgg cgattccgac cggtacggcg
     5461 atgacgatcg cggcgatgcc gaggaggaga gcctggccga aatagatgcg ggcgatctgc
     5521 cagcgcgttc cgccgatcgc cttcatgatg ccgatctgcc ggatctgtgc ggccatcaac
     5581 gcgctcagca gattcacgac gatgacgccg ctcaggatca gcacgaagag cccgaagctg
     5641 gatagagaca gcaggagcaa tcccatgatg tcggcgtgcg gatgtttgcc gggcgccgga
     5701 atgtcgacgc gcgtcaccgg atgccctcgg cgctcgatcg tccgcttaac atcggcagtg
     5761 acggcctcga tatgcggcag gtcaaaggcg tcacccgaga caacgaactt gatctgattg
     5821 agcaccggct cttcgccgag ctgcccgagc gtcgcctgag taatgtagcc gtagacaacg
     5881 ttctccatgc gcgcctgcgc ctgcccgacg tcgtgaacgc cgccactgac acggagtgtc
     5941 cgttccggtc cgttcgtcgt gcgcaccgtg accgcatcgc cgatatggat ccgcgcgacc
     6001 tgaaacgcat cgcgctcgat gaggatctcg cccgttgccg gtggccacgc gccctgctcc
     6061 ggattcaagt tgctgacgcg aatccgtccg aagtctttta cgacgaacag cgtcaggttg
     6121 cgccagccgc tcgcccctct gatcctcccg ctcaccgtcc gccgcgcttc cacctcggcc
     6181 acgcccggag ttgccgcgat cgcggcgacg agcccttcat cgaccgcgtc ggtgcgcagt
     6241 gtcgccgacg ccggattggt cgccaggtag ccgcggttca gctcgcgcgt caggatggcg
     6301 tagctcgcca gcaccgccgc gaatccggag atgccgaccg cgatcgcgag cacgacgaga
     6361 accgtccgcg tgctctcctg ccagaaatcg cgaaccgcct tcttccagcg cgtcgtcatg
     6421 cgccggtccc cgcggcgact gccagcggcg caggcgcggc tggaaggagg acaccgtccg
     6481 ccagctcgat ggtgcggtcg atgacacggg caacgtcgcg gtcatgcgtc gcgatgagaa
     6541 ccgtcgttcc gctgctggcc atcgcgcgaa agagatcgag caccgcgccg gcgttggctg
     6601 agtcgagatt gccggtcggc tcgtctgcga gcacgatcgg cgggtcgttc gccagggcgc
     6661 gggcgatggc gacgcgctgc tgctcgccgc cggaaagcga cgctggcaat ttgtccgcct
     6721 gcgctgagac gccgaagcga tcgagcaggg tcatcgcccg ccgccgccgt tcgcgcggag
     6781 gaaacgtccc gcagaaatcc atcggtagca tgacgttctc agcggccgtc aatgtcggca
     6841 gaagctgaaa gaactgaaag acgaatccga ccgtccgccc gcgccacgcc gcgagcctgc
     6901 tctcgccgag gctctggatc gacgcgtcgg cgatggtgac cgtgccggtg gacacggcat
     6961 cgattccgcc gatgacgttg agcagcgtcg acttgccgct gcccgatttg ccaacgacag
     7021 caaccagctc gccgcggaga acatcgagat cgagattccg gagcgccgtg aagccgcccg
     7081 cagtcgtttc gtaagtcttg gagacgtttc gaaggttgat catgccggcg acggtacgcg
     7141 cgcggccctg aaccaactct gaatgggacg ttcagcttgt attcagccag aagagactga
     7201 accacagaga cacagaggtc acagaggtaa acagccttct ctgtgacctc tgtgtctccg
     7261 tggtgatggc ttttgggaat caagcttgac ctgaatatga tgacggaatg aatcaaagcc
     7321 gctgcgcggt gctgatcgtc gaggatgacg acaagctgca ggaactgctg cgcacgttga
     7381 tctcgcggca ctgcacctcc gtcgatcttg cggcggatgg cgagcaggga atcgagatgc
     7441 tgcgcgcggg cacttacgac gccgtcatcg tcgatctgat gttgcccaag aagaacggcc
     7501 tacttgtgat cgatgcgatt gcgtcgcttt cctattcgcc gagagtcatc gtgctgtcgg
     7561 cgatctcccg ctattttcag gatcgcttcc ccgaggggac gatggtgctg cagaagccgt
     7621 tcgagatcga gaaggtggcg gacttgatcg ccaaccttca cgaaccgcat cctgaattgt
     7681 cgccgagcgc gacctgagcc gagattgcgc ccttactgcg cggcgcgtgc ggcggcgagg
     7741 acactggcga tcgctgcggt gagcgtgtcc gattccatcg gcttggcgag atacgcgtcg
     7801 aaaccttccg cgagaacgcg ctggcgatcg tcggcaccgc ggtaggcggt tgcggcgatt
     7861 gccggcgttc ggcggccgga cagcgcatcg cggcggcgga tctcggcgat cagtccatat
     7921 ccatccatct ccggcatggc gatgtcggtt acaaggatgt ccactgccac acgctcgaac
     7981 gtggcaatcg cctcggccgc agacgaagcg agcgtcgtct ccgcgccggc gcgcgaaaga
     8041 atcacgttcg ccaattcgcg tgcctcggac tggtcgtcga caaagaggac gcggacccca
     8101 gccagttccg cgctgcgctg ggcgccggcg ctcgcatcgg cgaccgagtg acgctgatcg
     8161 tcgctgacga ccgcgcgcgg agcggccggt aattccacga cgaagcgtgt gccgtggccg
     8221 ggaccatcgc tgaacgccgc aaccgtcccg ccgtgcgctt ccacgacgta gcgaacgatg
     8281 gccaaaccta gcccgaggcc gccgtgcgtg cgcgtcgtcg aagcgtctgc ctgacggaag
     8341 ggctcgaaca catgggcgag aaaatcgggc tcgatgccgc ggccagtgtc ggcaacggtc
     8401 aggcgaaccg tcgacggcgc gctcgccagt tgcaacgcca cgctcccacc ggactctgtg
     8461 aatttgatcg cgttcgtcag caggttccag atgacctggc gaaggcggtt cgcgtcgccg
     8521 aggacgatcg ccggagtctt gtcgggggcg acgagatgaa tcctcttcgc ggccgcggcg
     8581 acgcgcacgg cagtcagcgc gtcgtcgacg agcgtcccga ccgaaacgag ctcctgctga
     8641 aaatgcagct tgccggtggt cacgcgtgaa agatcgagca cgtcgtcgat gagtcttgcc
     8701 tgcgcgagcg cgctgcctcg aatggcctcg atgcctcgct gcaattcctc gccggtgacg
     8761 tcgggctggc tgacgattcc tgaccagccg aggatgatcg tcaggggcgt actcagctcg
     8821 tgggaaagga tggccaggaa ctcgtctttg gccaggctgg ccttctggat gtcgtcgtaa
     8881 agccgcgcat gctcgaccgc cagggcgatg cgccgcgcca catcctcaac cacccggagg
     8941 tcacgctccg acaatgcccg tccggagtga ctcatcgcca gcgtgagcgc accgatcggc
     9001 tggctgtcgg aaacggccag aggcacgatg agcgcggaac gaaaccctgt tgcgcgcgcc
     9061 tcggatccgg atagctgcgt ggccttcccc gttgcgatga cttccttcac aggatcactg
     9121 atcggcgcgg ggagttgatc tccatcgatc gcggcgagcc gttcggcgtg cagatcgatg
     9181 aagcagtggt cgcccagcag cggaaccatg agcttcgcga cgcgcgcgac ggtggcgtga
     9241 tagtcgagcg atgatgcgag cacggcgctc gcttcggcca tgaacgccgc ggtgcgctcg
     9301 gcgtacttgc gctcggtgac gtcgagcata gcgccgatca tccggatcgg cgtcccatct
     9361 tccgagcggg cgatatagcc ccgatccagc acctcggtga acgtgccatc ggcgcgctcg
     9421 aacctatatt cgtcggtcca ggacgagcca cccttctcga tgatgctgtt gatcccggac
     9481 acgacgcgct ctcggtcgtc tggatgcaga tggctatccc accactggag gcggttgtcg
     9541 atctggtcgg gctcgtaacc gaacatggcc cggagggcgt cgttccagat gatcgttccc
     9601 tgctcggcat cccagtccca gattacgtcg ttcgtggcgc gggcgacgag agcgtaccgt
     9661 tcgtgggcgc gccgcacttc atcggatgcc ttttcaatgc tcttctcttc ggtgatgtct
     9721 tcgatgattg cgatggcgaa gtcgtccgcg ccctgatgca ggagggtggc cgtcacgcgg
     9781 caccagacga cactgtcgcg cttgtggaga aggcgtttgt cgatcgagta gcgcgaacgc
     9841 tttgcagaca acaactcctg aaagagtgtg gtgcctgcct ggagatcttc cggtgatgtg
     9901 atggcgcttg cggtcagttg caacaactcg tcgttggagt agccgaggag ttgacgcagc
     9961 gctgcgttgg cgacgacgat ccggccgccg gcgtcgaaaa cggcgacgcc gacgccggca
    10021 tgctccagca cagcagggat caggctgacc gtcgcactct cgcgttcggg ctcttcgcca
    10081 tcggcagcag atgtcacggt ttgattatgc accgccaccg ggaatgacga gtgaaccagc
    10141 cgacgctgcg acgatgcccg cctacggtgc cgatgttgcg tcgtagtgcc gcacgaattc
    10201 gtcgggattg atcaggaact cgcggtcccg agggaggaag tggactttct cgatgtcctg
    10261 accggcgaat gctttgatcg cttcgcggtt tggccagtag gaaatgacga tgaattccgt
    10321 gatgtcacca tcggatctac gcaacatctc ggcacccaga tttccggcga tcatgcggat
    10381 cttcaggacg ccggtatcgt aaaggtactt ctcatattcg tctgcacgcg aagtggcgac
    10441 tctgcctcgc cacatgcgcg cgacgatgtg gactgcggtt ttcgcagttg cggggctagc
    10501 ggcttttgct ccatccgcgc gcggtgttgt ttgcgtggaa gcggggaacg ccagcaagag
    10561 caagaccaga agaatgctca tcgaaatctc ctccgagatg tatccaggaa tcgaggacca
    10621 ggattcgagt ccgcgctcag catagactct gcgagacctt gaaaacaccg tcaaacgaac
    10681 gccagcgcgc gttgcaaatc cttcgccgca tcgagcgcga gtcggcgtat gcgtcgctca
    10741 ttctgcagaa cgattcgggt ttcgtgcgca cgctcgtgct tggcgtgctt cgctggcggt
    10801 cgcggctcga ctttgtgatc gatgccttcg cgaagggcga catcgagccg gagattcgcg
    10861 acatcctccg catcggtgcg ctgcaactgc tgttcatgaa cgttgcttca cacgccgcag
    10921 tgagcgagac ggtgtcgctc gtgccgctgc gagcgcgcgg attcgtgaac gcgatcctgc
    10981 gacggatcgc atcgaagccg attcccgagc cgccagacgt ggcgacgcga acggcgcatc
    11041 cgcgatggct catcgaacgc tggacgaaga ccttcggtgc tgagcgcgcg gcgaagatcg
    11101 cggcggcgaa tcaggaattg tcgtttcccg acgtgatcac cgacgcgccg ccgtccggcg
    11161 cgacagaatc ggagctggtt ccgggaatgt ggaagctcac cggttcgagc gcggacgtcg
    11221 acggcatcgt gctcgacgag ggaagcgcgg tcatcgccgg catcgccgcg gccctcggcg
    11281 acgatgtgct cgatctcgcg gcagcgccgg gtggcaagtc acgcgtgatg cgtactcgtg
    11341 gcgcgcacgt cgtcagcaac gatgtctcga tctcgcgtct tcggccgctc atcggaaaat
    11401 cggcgtccat cgtcgtgagc gacggccggc aacctccgtt cgcgcggcga ttccgtgtcg
    11461 tcctgctcga cgcgccgtgc agcgccaccg gcacgatccg caagaatccg gagatcaaat
    11521 ggcgcctcca ggaagcggac ctcattgcgt tcgcgtcgct gcaacgggag ttgctggcat
    11581 cggcgctcga tctcgcgtcg gagtcggtgg tctacgcgac ctgctcgctc gaacccgagg
    11641 agaacgatga cgtcgttggc gaactgctgc gcaaccgcac ggacttcgtt cgcggcgatg
    11701 tcgctgatct cgtgaatgag aacgttgccc gctgggtgga aaacggcgtc ctccgcctca
    11761 ctcctgaagc ggggaccgat ggattcacgg cgcactggtt acgacgaaca tgacgagcgc
    11821 ttgatcgcaa ctgaatctga gtcgcagcgc gcgaatgaaa tcttcgtttc ttactccaaa
    11881 acagcgcaaa aagaagtgat gcgagccccg aaaaagcgtg cgtgtgctct tgcgttgaca
    11941 aaccaagatc catctcatag attgcaaaaa acctagaaaa catcagcgtt tcaggcattt
    12001 gaagaaggag aacgtctatg gcaggcaagg gtgacgtcat caatgaaatc gccgaacagg
    12061 cgggtatttc gaagaaagag gccgcggccg cgttcgacgc gttcgtgaag cacatctcgg
    12121 actcgtgcca gcgcggagac cgctgtgcca ttccggggct cggcagcttc gccgtgtcgc
    12181 agcgaaaggc gcgcgaaggc cgcaatcccc gcacgaacga aaagatcatg attcccgcgt
    12241 cgaagaacat ccgtttcaag gcgggcaagg acctccgcga agcggtgaat tcgaagcgca
    12301 agggcaagaa gtagaacctc cacccttctt tgccgtgacg aaacgccccg cccgaggcgg
    12361 ggcgttttca ttggagcacg tcgtcgagcg agcggcacac gcggaccgcc atcgccgcga
    12421 tttcgggtga gggctcgacg aggtcgggca ccatgatcac ggccatcgcc gccgcacgag
    12481 cggcacgaac gccggcctcc gagtcttcga tcacgaggca atcatccggc gcgatgccca
    12541 gacgcgatgc ggcgagcaga aaaagatcgg ggtaaggctt cgagtgctcg acttcatcgc
    12601 cgaacgcgaa gacggaaaag cgtgagtaca ggcttcctag acagcgcagg gcaacggcac
    12661 gtggcgtcga tgtggcgagc gctttcggaa tcccgcggga atcgagacga tcgagcagcg
    12721 ccactgagcc cggcttgtgc gggagcggcg cgtgcgcgag acgttcgtcg aaatcacgtt
    12781 tccaccggac tctgaactcg tcgagcggca tcgcgtcgcc gaagcgctcg atcaacagcc
    12841 gctccgcttc gtcattgccg cggccgacga atcgcgaata gaagtcgtcg tcgatcggac
    12901 agcccagctc tgccgccgct gtctgccatg tgacgcggta gagcggttcg ctgtcgagca
    12961 gcaggccgtc catgtcgaag atgacggcgc gcggcgtcag aacattggtc aaacgaacgc
    13021 tctcggaacg aagagtcgtt catagagatt gatcgcatac cggtcggtca tgccggcgat
    13081 gaagtcgatg gccagccgtt cgaccggctc gtcgctcgtt gcgctcaaga agtcgcccgg
    13141 gttggcggtg acatgctcga agagcgcagt gagcgttcgt tgtgccttca cgaactcgtt
    13201 gcgcaccgcg ggtgtgaggt acatgttctg aaacatgaac gtccgcaact cctcgagcgc
    13261 gcccagagcc tcggcagaca tcgagatagc ttcgtagccg caggcaagcg tcgaatcgat
    13321 cacgtcgcgc accatgcgat cgatccgctg cgcggctccg ttgccaagcg tcttgcggat
    13381 ttctttcgga atctcgttct cggtcagcac gccggcgcgg atcccgtcgt cgatgtcgtg
    13441 gttgacgtac gcgatgatgt cggagatgcg gacgatgtcg ccctcgaggg tgagcgcgcg
    13501 cgacttcgcc cgtttgcgca acagctcccc cttctcgcct ttcgagtgtt tgaggatgcc
    13561 gtcgcgaacc tcgatagtga ggttcagtcc gcgtccgtcg ttctcgagca cttcgacgac
    13621 acgcaccgac tgacggtagt gatcgaaccc gcccttcacg agcttgttca acgctgcctc
    13681 gccggcatgc ccaaacgcgg aatgcccgag gtcgtggccg agcccgatcg cctcggtcag
    13741 cacttcgttg agccgtagcg acttcgcgat cgtccgcgcg atctgggtta cttccagcac
    13801 gtgcgtgagc cgggtccggt agtgatcgcc ctccggtgcc aggaacacct gcgtcttgtg
    13861 cttgaggcgg cggaaggctt tgcagtgaat gatgcggtcg cgatcgcgct gatagctggg
    13921 acggagatca tcctccggct cgtcgcggtc acgcccgcgc gtctccgaac tgcgcgccgc
    13981 ctgcgcgacc atcgtgtcgc gctcgatgtt ctcgagcatc tcgcgaatgg tggtcatcac
    14041 gcgcacttta tcgcagggag tgcgagcgtc tcgctcgcgg ccgtcgggcg tcctcgcccg
    14101 atggtgtggg gtggacttgg cgccgcgggt gggtttcgtg cgtaatcatc ccctcacgga
    14161 aacttgggac atcaactcct cggaatcact gccggacgag acgtccggca gccgcaggcg
    14221 agacgcctgg cgagacgcct gcactcctca ggcgcagttc catgaacttc gttccttcga
    14281 ttggattgaa acgatacggt gggatctcga cgaagccgag ggacttgtag agcgggatag
    14341 cggcggtcat cgttggcaga gtgtcgagac gcatgcgcgt gtagccgcgg tgccgagcct
    14401 cgtcgatgat gcgttccgcg agtgtgcggc cgaggttgcg tccgcgaaat tcggggcgaa
    14461 tgtagaggcg cttcatctcg cacgtggtgt cgtcgaggcg gcgcagtgca acacaaccgg
    14521 cggcacgatc gccttcgtgt gcgatcagaa ccagctcgta gaagtcgccg agggtgctca
    14581 gctcgtgatc gagatcctga aatgagagat cgacgccgag ggaggatgca tattcgagaa
    14641 tgagctcgcg gacgacctga agatcgttgg cagtttcgat cgcgatcaca ccacgagctc
    14701 gccgcgcaac accgtgacgg cgtgaccggc aagctttacg cgatcgcctt ccagccgcac
    14761 gcgcacggcg ccgccgcgcg gagaggcctg ccacgcattc atttcgttct tgccgagacg
    14821 ttgcgcccaa taaggagtga gacagcaatg cgctgaaccg gtgactggat cttcgtcgac
    14881 acccgatccc ggggcgaaaa agcgcgagac gaaatcgtat tcgcctgcgt ccgcgcggct
    14941 ggtaacgatg attccgcgca cgggaagctg acgcagcacg ccatggtcgg gccgaaggtt
    15001 gtgcacgtct ttctcggacg gcagctcgac gatgtagtcg aactggttgc ggccgacgta
    15061 ggttgcctgc gtggtgccga gggcgtcgag aagtcctgcc ggcgcagcaa tctgttcatc
    15121 gcgtttggcg gggaagtcga gctcgatgaa gtcgccgtct cgggccgcag tcagcacgcc
    15181 gctcttcgtg tgaaaccgca gcaccgcctc gctgctgatg ttctcgctcc agatcgcgtg
    15241 tgcggacgcc agcgtcgcgt gaccgcacag atcgacttcg accgtaggtg taaaccaacg
    15301 cagcgaccat ccatcctcgc gaggccggag aaacgccgtc tccgcaagat tcatctccgc
    15361 cgccacattc gccatccacc ggtcatcccg ctcaccgtcc atgaaacaga cggcggcggg
    15421 attgcccttg aatggctcgc tggtgaaggc gtcgatctgg agaaggcgca tttcgcggtc
    15481 tcgcagtgtc gcgaatcaga atccctgaac cgccgccgcc tttgcgtaca tccagcggac
    15541 gaagaggaag ccgacgatga tcacgccggc gaggatgccg tcgcgaaccc acatcgacgc
    15601 gccgtaaacg gtcgggtagt gattgctgat catcaggaag atcagcggta cggacatgta
    15661 cgtgttctgg cgcgagcgca ggccggcctg gcgcacggtc tctgcgtccg cgggattgcc
    15721 gtccttgatg ccgcgaatga ttttctgctg ggcgggccag atgaccatcc aaacattcag
    15781 ggccatcatc gtgccgaaga tcccgccgga atggatgtag agcgcgcgcc cgctgaaatg
    15841 cccggcggtg gccaggaggc aatagacgcc ggcgaagagg accatgttga tgatactggc
    15901 gacagtaacg ttcttgacca tcttcaggat ctggttgtag atcacgaaac cgaccgcgag
    15961 cgtgatgaga acgacggcca gccagagcca cggattgccg acgtggtcct gatcgaacag
    16021 aaccagcttg gtctggtagt agacgaggcc gagcagcagg aagccggtga tccacgtcca
    16081 cgcggcaccc cagcggaacc agaagagcgc acggggcatg agctccggca cgacgagttt
    16141 cttggtcggc ccgtccatct tgcccgcgaa cggtccgttg acgaagttga agaaccagag
    16201 gtgaccgatc cacatcacgc cggcaacgac gtggccccac cggaaaatga gttgtagcca
    16261 gttgttgatt tcgggattca tatgctctcc tcaatcgctg cggtttgggg tagcctgccg
    16321 gcgattatat gtcgcccacc atgtcgcctc acgacttcga gaatcgtgtt gccatcgtca
    16381 ccggaggctc aaccgggatc ggccgtgcca cggcggagat gttggcgaag cgcggcgcac
    16441 gcgtggcgat tttcgcgcgc tctgcctccg catccgggca acttcgcgaa aacacgtttg
    16501 ccgttgacgg ggacgttagc gacgaagccg caatcgagcg gctgtttgcc gagacggaat
    16561 cgcgcttcgg cgattgcgac gttctcatca actgcgccgg aatgatcgat ccgaaagcgc
    16621 tcatcgatac agttcccgat gactgggacc ggatgttcgc ggtcaacgtt cgcggaacgt
    16681 acctggccac acgacgcgca ttgccttcga tgattgcgag gcgtgccggc tccatcgtca
    16741 acgtcgcctc gatctcaggg gtcatcggac cggagaagtt tccgggatgg gtctcgtact
    16801 gcgccagcaa agcggctgtg atctcgatga ccgaggcgct cgcagtggag gtcaaagagt
    16861 tcggcgtgcg cgtcaacagc gtgagcccgg gatcggtcga cacgaagatg tgggctgcgg
    16921 ccagcggcgg cgcgcccgct gcaatgacag cggatgaggt tgccgaggtc atctgcttcc
    16981 tcgcgtcgga ccgggcgcgc gctgtgaacg ggcaggacgt gcatgtctac tcttcgtgaa
    17041 ttgcggccaa cattcgccac gatcgatctc gaacgctttg cgcacaacgt cgatgtgatc
    17101 gcacgtttgc tgcccgaggg cgcgaggctc gtgccggtgc tcaaagccga cggatacggc
    17161 cacggcgcgg tcgagctggc gaaacggctg acgcccgatc gcgtggcgat gatcgcggtc
    17221 gcgctgctcg aagaagcgtt ggaactgcgg cgggcttcga tcacgctgcc gatcctggtg
    17281 ctgggaccga tatcgcgcga gcagatttcc atcgcgctgg atcatgacgt gacgatcggc
    17341 gtgatcggac cggaggagct cgagtcagtc tgcgatgtgg ctcgcgatcg cgatgtggcc
    17401 attcacataa agctcgattc gggcatgggg cggatgggtg tgatcgagag cgaattgcca
    17461 cgagtcattt cgatgattcg cgcagcttcg cggctgcgta tcgacgggat ctacacgcat
    17521 ttcgcgaatg ccgaagatgc aagtgatccg ttcacggaac agcaaatcgc gacgtacgac
    17581 cagatgctgg ccgtgcttcg cgagcacgga atcgatgcgc ctcggcatca cttcgccaac
    17641 tcaccggcga cattgaatgg actcgttcgg cccagtgatt tcgcgcgcgt cggcatcgcg
    17701 ctttatggtc ctcaacg
//
LOCUS       DATLVW010000100         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_375581, whole genome shotgun sequence.
ACCESSION   DATLVW010000100 DATLVW010000000
VERSION     DATLVW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375581"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VNN25_03420"
     CDS             complement(<1..925)
                     /locus_tag="VNN25_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXH90606.1"
                     /translation="MRTRATLRAATLFVTGMAVLTPLHAYFAEWVRTSTQSVFNPERP
                     LTADFNGDGRADVVVQTGNTALRVLITNPDGTLGTPTLLYQSDYLSSTILADVDGDGR
                     KDVVTADVATNTLIVIPSNGDGTFGIPIETAVPIAPTEIAAGDFNGDGKLDLAIRSYS
                     ADILAIYAGDGSGHFSEIHREPLNNLPYQIAVGDVDGDGNLDILISDQQPAGHELRFG
                     KGDGTFEAPVSISGSATASSSAILADLNGDGRLEILTTEFNPNTVTVINNVSGRTFAA
                     PVSYLVVPSSLSPYGNPINLVVADFNDDGKPD"
     gene            complement(922..>2354)
                     /locus_tag="VNN25_03425"
     CDS             complement(922..>2354)
                     /locus_tag="VNN25_03425"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH90607.1"
                     /translation="SKYLLKVYRNDCGDSSFTATTESPTISAGQAATLNIALLPPVDV
                     FAYFGTTGQFTIKEGAQVLATASFQFGRGSVTINGLSLGNHALTASYDGDPQYGATTA
                     APLTLHVTNATTTTAVSIDPAAPVYSQGAQIHATVMSSTGDTPGGPIRFVIDGQSTFY
                     SPGTAPQATTYGPTTVGSHTVVVNYIGDATHPPSSATQTYVVSKQKPQITLGLASTAA
                     NAAFNTSLYLSSAFSGSSNPTGSVTLSEGATSLGTKSLDSYPLVYFDFPPLAPGRHEF
                     RVVYTGDANYQAMDTFLTIIAFATTSASVDARGNANNISVVWYPTSFIAVSRKTPGQA
                     WTKRMCCSPPPFTDSIGVQPETVYLYRAESYDGSSLSNVDVGMRISFTDDPLLPGAVM
                     RSVYLQEIVHAANLLRAAANLSAISASPFVAGAPVTAAGINLLRTAINEARVSLGAYP
                     FAFTGTPATGVMVGAADIRELREAVR"
BASE COUNT          441 a          641 c          811 g          461 t
ORIGIN      
        1 cgtccggctt gccatcgtcg ttgaaatcgg ccacgacgag attgatgggg ttgccatagg
       61 gactgagact ggacggaacc acgagatacg agacgggagc ggcgaaggtt cggcccgata
      121 cgttgttgat gacggtgacc gtgtttggat tgaactcggt cgtgaggatt tcaagacgtc
      181 cgtcgccgtt caagtcggcc aggattgctg acgaggacgc cgtcgctgag ccggaaatgg
      241 aaaccggcgc ctcgaacgtg ccgtcgcctt tgccgaaacg gagctcatga cctgccggct
      301 gctggtccga gatcagaatg tcgaggtttc cgtcaccatc gacgtcgcca acggcgattt
      361 gataggggag attgttcagc ggttcgcggt gaatctcgga gaagtgaccg ctgccgtcgc
      421 ctgcgtagat ggcgaggatg tccgcgctgt agctccggat tgccagatcg agttttccgt
      481 cgccgttgaa gtctccggca gcgatctcgg ttggagcgat cggaacggcg gtctcgatcg
      541 gaatgccgaa tgtgccgtcg ccattcgaag gaatgacgat caacgtgttc gtggccacat
      601 ccgccgtgac cacgtcctta cgcccgtcgc cgtcgacgtc tgcgaggatc gtgctcgaca
      661 gatagtcgga ctggtagaga agcgtaggtg ttccgagtgt gccatcggga ttggtgatga
      721 gtacgcgcag ggcggtgttc ccggtctgaa cgacgacgtc ggcgcggccg tcgccgttga
      781 agtccgcggt gagcgggcgt tccgggttga ataccgattg cgtgctggtg cggacccatt
      841 ctgcgaagta cgcatgcagg ggcgtgagca cggccatccc tgtgacgaag agtgtggcgg
      901 cgcgcagcgt tgcacgcgtc ctcatcgcac ggcctcccgg agctcgcgga tgtctgcggc
      961 accgaccatc actccggtcg ccggcgttcc cgtgaaggcg aacggatagg cgcccaggga
     1021 gacgcgtgcc tcgttgatgg ccgtgcgcaa caggttgatg cccgcggcgg tcaccggagc
     1081 tccggcgacg aagggagaag cgctgatcgc ggagaggttg gctgctgccc ggagaaggtt
     1141 cgccgcgtga acaatctcct ggagataaac cgatcgcatc acggcacccg gcaggagtgg
     1201 atcatcggtg aacgagatgc gcatccctac gtcgacgttg ctcagcgagc ttccgtcgta
     1261 ggactccgcc cgatacaggt aaaccgtctc gggttgtact ccgatggaat cggtaaacgg
     1321 tggcgggctg cagcacattc gtttggtcca agcctggccg ggtgtcttcc gggaaacggc
     1381 gatgaagctc gtcgggtacc agaccacgga aatgttgttc gcgttgccgc gggcatcgac
     1441 cgacgcagaa gtcgtcgcaa acgcgatgat cgtcaggaag gtgtccatcg cctggtaatt
     1501 cgcatcgccc gtgtagacga cgcgaaactc gtggcggccc ggagcgaggg gtgggaagtc
     1561 aaaatagacc aacggatacg agtcgagcga ctttgttccg agggacgtcg ctccctcgct
     1621 cagtgtgaca ctccctgtcg gattcgagga accggaaaat gccgaagaaa ggtagagcga
     1681 tgtgttgaat gcagcgttcg ccgcggttga cgccaggccc agcgtgatct gcggcttctg
     1741 cttcgaaacg acgtacgttt gcgtggcgct gctgggagga tgcgtggcgt cgccgatgta
     1801 gttcacgacg acggtgtggg agccaaccgt cgtcgggcca taggtcgtcg cctgcggtgc
     1861 agttcccgga ctgtagaagg tcgactggcc gtcgattacg aaacgaatcg gtccgccggg
     1921 ggtgtcgccg gtgcttgaca tcacggtggc gtggatttgg gccccctggc tgtatacggg
     1981 tgcagccgga tcaatcgaca ccgccgtcgt cgtcgtggca ttcgttacgt gaagagtgag
     2041 gggtgccgcg gtggtggcgc cgtactgcgg atcaccatcg taagaggcgg tgagggcgtg
     2101 gttaccgagg gagagaccgt taatggtcac cgaaccgcgg ccgaactgaa acgaagccgt
     2161 cgcgagtacc tgcgctcctt ctttgattgt gaattggccc gtcgtgccga aatacgcaaa
     2221 cacatcaacg ggagggagga gggcgatgtt cagcgtcgca gcttgtcccg ccgagatcgt
     2281 cgggctctcg gtcgttgccg tgaaggacga gtcaccgcag tcgttccggt agaccttgag
     2341 cagatactta ctcg
//
LOCUS       DATLVW010000101         5342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_541038, whole genome shotgun sequence.
ACCESSION   DATLVW010000101 DATLVW010000000
VERSION     DATLVW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541038"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VNN25_03430"
     CDS             complement(<1..313)
                     /locus_tag="VNN25_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90608.1"
                     /translation="MHATQGSAERRSSGTSGVGILRKSARALPIAAACASAGIGTAPL
                     TAESNNPVGMSSNRPDSERLMARQVRRNGRIHARPRSRSAAHERGFARRKLRAFFPMQ
                     RF"
     gene            444..1028
                     /locus_tag="VNN25_03435"
     CDS             444..1028
                     /locus_tag="VNN25_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90609.1"
                     /translation="MSSSVEVPVTPPQPSYAESALALVEELRAMRERIPHLVIPETRD
                     ARRRLANAASVPPEFIELTSMAVKSHAPLVRGGGQDLAQNTDLKSFAEAYGPLGDELE
                     ALTSFVKHSVTRAMNTVGSEALTTYALALRLSKRPESADLIPHVDAMRKALGKRIRKS
                     KSKPAPKPANPVTAAPEETSPVTPSSPTTDPKTK"
     gene            1463..2779
                     /locus_tag="VNN25_03440"
     CDS             1463..2779
                     /locus_tag="VNN25_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXH90610.1"
                     /translation="MLDHLKAIYLRTLDACAPERLVRAVVTYDMPRIVVAIGKCGGAL
                     RDGLDDVDEALVVVPLGYRMPEKRSRNAIGGHPDITSASVDAGRQLLEFVDAHDEILF
                     LISGGGSACVELPLTPWFDERDLIETNAKLVASALKIGDINCVRKHLSAIKGGRLAAR
                     VKRSVTLVYSDVSTGALADVASGPTLADTTTKADAIAILERIGGCDRIVAKLRDQECP
                     ETVKHIDDSRFALIADNRTLVATAARITSSMGFVPVVVEQQIESEIGDAANDLLERAS
                     RLRAGEVLIAGGEPTLVRRGDGKGGRCIELAVRMALAEAERRSDSPVRLPERRSDSLV
                     RLPVGSEREPSDWRVRPTSVSDTSVQSSSILPSIHALLGTSDGVDGNSGVAAIAVTLP
                     ASIDREVAERELLRSNSLAVAEEIGETIMIPPAGNNLRDLYLLART"
     gene            2804..>5342
                     /locus_tag="VNN25_03445"
     CDS             2804..>5342
                     /locus_tag="VNN25_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXH90611.1"
                     /translation="MTIFPAPEEHAEHTEPVHRSARERQRRGPMWGCLRGVGCTAIGF
                     FALLTIVIVGGWWYLGSSSFAGLVRLRIEKTLEARLGRDVYVGPVTIERGTQSRIIIN
                     GLRVANAPGAKHPYFATASQIIVTGGIDSFWGRKIRVGRVDIVEPHLYFEIYPAGAKL
                     THNFPHWNSGPPSRYEIYHLDLGNLYVTRGVFDFLDRRHDIAAVTSDITSTISVTPLE
                     DLYAGVVSSPQFTLRIQDYVPMTTSLRGEFRFTPDDLELRSMALEGGPDLRVFLNGHV
                     TPLSDAVYNLHVTSQIGLNRVRQIFKVNKTLDGTIDLDGMLRGKAGTFALTGGWLSPK
                     MKADVYDLTNARGTLKITGDRTDVDIQRATYAGGTLAGKYALPQYNEPYAMSVELRYK
                     DIGIEKLFNDLGIKDTGLRSAATGTLNHYWEKDRVLAGVGDGKATLTKSGTAFSNATY
                     PIPIAGSVDFKIENKYFQFKRADLVTDASTIALDNGSFEMETGSTQLSAKIDSRDFSE
                     LDRIGYNFAHSAGKNSYTLLGLGGSGTITGSIQGVLKSLRVSARITSTGAKYNNVLLG
                     DLDMDLLYEGSRALGFDKRGGVIYAEKNLLTFEKTTIRDGAARLGMTGTIFFPDSGPP
                     QFDLALDAAGYNVERAMHAVDLKLAIKGAGTGHLVVTGTADAGKVIFANFLVHQAKGD
                     LRLNGTVAWAPGKGTTNFDLDVATTNFPVADIITFLDLGTLPITGDVSGKLHLSGPKA
                     TLGGTGQVTISNGSIYGEPVTSAKTDITFTKGALKAANVTIVSPAGTITGEAQIDFGT
                     NQFSYTIKSSSIDLSKLKLLSSLANILGGNVTLSSTGAGTLDQPELVL"
BASE COUNT         1068 a         1715 c         1596 g          963 t
ORIGIN      
        1 cgaaacgctg cattgggaaa aacgcgcgca gtttgcgtcg cgcgaagccg cgttcgtgcg
       61 ctgcggagcg cgaacgaggc cgggcgtgga tgcggccgtt ccgccgcact tggcgcgcca
      121 tcaatcgttc gctgtcaggc cggttcgacg acatccccac cgggttgttc gactccgcgg
      181 tgagcggcgc cgttccaatc cctgccgatg cgcaagcggc ggcaatgggc agagccctcg
      241 cgctcttccg caggatcccc acaccactcg ttccactcga tctcctctcc gccgagcctt
      301 gcgttgcatg cattcctgct cgtcgacggt ccgttcgcgt agccgtcgtc gaagcaacaa
      361 gacggatctc cagagggcgc gcccctccgt ccgcagcaac ctgctcgctc tgatcacagc
      421 agtcccgaaa cgtggaggct ccgatgtcct catccgttga agtacccgtc acacccccgc
      481 agccgtcgta tgccgagtcc gcgctggcgc tcgtcgaaga gctccgagcc atgagagaga
      541 ggattcccca tctcgtgatc ccggagacca gagacgcgcg ccgccgcctg gcgaatgccg
      601 cctcagttcc gccggagttt atcgagctga cgtcgatggc agtcaagagt cacgcgccgc
      661 tggtccgagg aggaggccag gatctggcgc agaacaccga tctgaagagc tttgctgagg
      721 cgtatggccc gctgggtgat gagctggaag cgctgacttc cttcgtcaag cacagcgtaa
      781 ccagggcgat gaacacggtc ggaagcgaag cgctgacgac ctacgccctg gcactccgtc
      841 tgtccaagcg tcccgagtcg gccgatctca ttcctcatgt cgacgccatg agaaaggccc
      901 tcggcaagcg cattcgtaag tccaagtcca agccggcccc gaagccggcg aacccggtta
      961 cagcggctcc cgaggaaacc tcgccggtga cgccgtcgtc accgacgacc gatccgaaga
     1021 ccaagtaacc ggtcgcgtgc agtacgtagt aagtcccgcg cgggcgcgtc cgaaggcgcg
     1081 cccgcgcatt ttgtttttgg ggaggcttca atgctgcgta aagggcttcg atgcgcatca
     1141 cgcgcaaagt gtcatcctga gactgccgaa gccgccacgc gcattcgaac ccttcgcgct
     1201 gcagaaagtg gcggcgtagg acggcgaagg atctcaattt acgaggcatg tggcatgtgc
     1261 atcgtatctt gagatccttc gccgtcctgc gccgcctagc acattctgcg cgagcgggat
     1321 cgattcacgc gggcggctcc ggcagtctca ggatgacacc acggaggaga ccgcgagagg
     1381 cgacgctgat tttgtaactc cttgtaattt cttgtttctt atcaggctcc aacttccgta
     1441 atccccgtaa gatcatcgtg acttgctcga ccacctgaaa gccatctacc tccgcacgct
     1501 cgatgcgtgt gcgcctgagc ggctcgtgcg cgcggtcgtg acgtacgaca tgccgcgcat
     1561 tgtggtggcg atcggcaagt gcgggggggc attgcgggat gggctcgatg atgtcgatga
     1621 ggcgcttgtc gtcgtgcccc tggggtatcg gatgccggaa aagcgatcgc ggaatgcgat
     1681 cggcgggcat cctgacatta cgagtgcgag cgtcgacgcc ggcagacagt tgctcgaatt
     1741 cgtcgatgcg cacgacgaga tcctcttcct catctccggc ggcggatcgg cgtgcgtgga
     1801 gctgccgctg acaccgtggt tcgatgaacg ggatctgatc gagacgaacg caaaactggt
     1861 cgcctcggca ttgaagatcg gcgacatcaa ctgcgttcgc aaacatctct cggcgatcaa
     1921 gggagggcgg ttggcggcgc gggtgaagcg gagcgtgacg ctcgtttact ccgacgtctc
     1981 caccggcgcg ctggcggatg tggcgtcggg gccgacactg gctgatacga ccacgaaggc
     2041 ggacgccatc gcgatcctcg agcgcatcgg aggatgcgac cggatcgtcg ccaagctgcg
     2101 ggatcaagaa tgtccggaga cggttaagca catcgatgac tcgcgcttcg cactcatcgc
     2161 ggacaaccgc acgctcgtcg caacggccgc gagaataacg tcatcgatgg gattcgtgcc
     2221 ggttgtcgtc gagcagcaga tcgagtcgga gatcggcgat gccgcgaacg acctgctgga
     2281 acgagcgagc cgtctgcgag ccggcgaggt gctgatcgcc ggcggcgagc cgacgctcgt
     2341 gcgccgcggg gatgggaagg gggggcggtg catcgagctg gctgtgcgca tggcgctcgc
     2401 ggaagccgaa cgtaggtcgg actctccagt ccgactaccc gaacgtaggt cggactctct
     2461 agtccgacta ccggttggtt cagagagaga accgtcggac tggagagtcc gacctacgtc
     2521 agtctctgac acgtcagttc aatcgtcgtc aatccttccg tcgattcacg cactcctcgg
     2581 cacctccgac ggcgtcgatg gcaacagcgg cgttgccgcc atcgctgtca cgcttcccgc
     2641 atcgatcgat cgcgaagtcg cagaacgcga actgttgcgg tccaacagcc tggcggtagc
     2701 cgaggagatc ggggagacga ttatgattcc cccggccggc aataacctcc gtgacctcta
     2761 tcttttggct cgcacttagc aggagactga cggcctcaac gaaatgacga tcttcccagc
     2821 acctgaggag cacgcggagc acaccgagcc cgttcaccgc tcggcgcgcg agcggcagcg
     2881 gagaggcccg atgtggggct gtctgcgcgg ggttggttgc accgcgatcg gcttcttcgc
     2941 gcttctcacc atcgtcatcg tcggcggctg gtggtatctc ggatcatcga gcttcgccgg
     3001 actggtacgg ctacgcatcg agaagacgct cgaggcgcgc ctgggacgcg acgtctatgt
     3061 cgggccggtc accatcgagc gcggaacgca gagccgcatc atcatcaatg gcctgcgcgt
     3121 cgccaacgcg cccggcgcga agcatcccta ctttgccacc gcctcgcaga tcatcgtcac
     3181 cggcggcatc gactcgttct ggggacgcaa gatccgcgtc gggcgcgtcg acatcgtcga
     3241 gccgcatctc tacttcgaga tctatcccgc cggcgcgaag ctgacgcaca acttcccgca
     3301 ttggaactcg ggtccgccgt cgcgctacga aatctaccac ctcgacctcg gcaatctcta
     3361 cgtcacacgt ggcgtgttcg acttcctcga ccgccgtcac gacatcgccg cggtcacgtc
     3421 ggacatcaca tctacaatca gcgtcacgcc gctcgaagac ctctacgccg gcgtcgtcag
     3481 cagtccgcaa ttcacattgc gcattcagga ctacgttccg atgacgacca gtctgcgcgg
     3541 agagtttcgc tttacgcccg acgacctcga gctgcggtcg atggcgctcg aaggcggacc
     3601 cgatctgcgc gtctttctca atggacatgt cactccgctc agcgacgctg tctacaacct
     3661 gcacgtcacg tcgcagatcg gactcaatcg tgttcgccag attttcaaag tcaacaagac
     3721 gctcgacggc acgatcgacc tcgatggcat gctgcgcggc aaggccggaa cattcgcgct
     3781 caccggcggc tggctttcgc caaagatgaa agcggacgtc tatgacctaa ctaacgcgcg
     3841 cgggacattg aaaatcaccg gcgaccgcac cgatgtcgac attcagcgcg ccacatacgc
     3901 gggcggcacg ttggccggca aatacgcact gccgcaatac aacgaaccgt acgcaatgag
     3961 cgtcgagctt cgctacaagg acatcggaat cgagaagctc ttcaatgacc tcggcatcaa
     4021 agacaccggt ttgcgcagcg cggcaacggg cacccttaat cactattggg aaaaggaccg
     4081 agtcctcgcc ggcgtcggcg atggaaaggc cacgctgaca aaaagcggca cggcgttttc
     4141 caacgcaacg tatccgattc cgatcgcagg ttcagtcgat ttcaagatcg agaacaagta
     4201 cttccaattc aagcgcgcag acctcgtcac cgatgcctcg accatcgccc ttgacaatgg
     4261 gtctttcgaa atggagaccg gctcgaccca actatcggcg aagatcgata gccgcgactt
     4321 cagcgagctc gatcgcatcg gctacaactt cgcgcactcc gccgggaaga acagttacac
     4381 gctcctcgga ctcggcggca gcggcaccat taccggctcc atccagggag tgctcaaatc
     4441 gctgagggtg agcgcacgga ttaccagtac aggggcgaaa tacaacaacg ttcttctcgg
     4501 cgatctcgac atggatctgc tgtacgaggg ctcaagggct ctcggcttcg ataagcgtgg
     4561 tggtgtcatc tacgccgaga agaatctgct gactttcgaa aagacaacaa tccgggacgg
     4621 cgccgcgcga ctcggcatga ccggcacgat cttcttcccc gacagcgggc ctccgcagtt
     4681 cgatctcgcc cttgacgcgg ccggctacaa cgtcgagcgc gccatgcacg ccgtcgatct
     4741 gaagctcgcc atcaaaggtg caggcacagg acacctcgtc gtcaccggca cagccgacgc
     4801 gggcaaagtc atctttgcaa atttcctcgt acaccaggca aaaggcgatc tgcgcctcaa
     4861 cggaaccgtt gcctgggcgc cgggcaaagg gacgaccaac ttcgacctcg acgtcgccac
     4921 aaccaacttc cccgtcgccg acatcatcac cttcctcgac ctcggcacgc tgcccatcac
     4981 cggcgacgtg agcggtaagt tgcacctctc cggaccgaag gcaactctcg gcggcaccgg
     5041 gcaggtcacg atcagcaacg gctcgattta cggcgagccg gtaacaagtg cgaagacgga
     5101 tatcaccttc accaagggcg cgctgaaggc ggccaacgtg acgatcgtct cgcctgccgg
     5161 cacgatcacc ggcgaagcgc agatcgattt cggcacgaat cagttctcgt acacgatcaa
     5221 gtcgtcgtcg atcgatctct cgaaactgaa actgctgtcg tcgctggcga atattctcgg
     5281 cggcaacgtc acgctctcat caacgggcgc cggcacgctg gatcaacccg agctcgtgct
     5341 cg
//
LOCUS       DATLVW010000102         4393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_375660, whole genome shotgun sequence.
ACCESSION   DATLVW010000102 DATLVW010000000
VERSION     DATLVW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375660"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VNN25_03450"
     CDS             complement(<1..212)
                     /locus_tag="VNN25_03450"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90612.1"
                     /translation="MRNAINVALAIVSGVLFAWSFPNVAAGWLMFIALLPLFVALTRV
                     NSYKGAFALGWLSHGVAWLLMVPWVIR"
     gene            complement(296..706)
                     /locus_tag="VNN25_03455"
     CDS             complement(296..706)
                     /locus_tag="VNN25_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90613.1"
                     /translation="MFADRSKILIVLLLAIACGRREPLTTRELVGQYYLYVNSDYTNP
                     NQPDDRLLLKADGAYEHSFVSGQAHRSSTGTWRVNGTHVVINDWQDYAGITSLQPLGD
                     RKLHYIVFTEGTPPTIVLDSDQNIFYDRRPHQHK"
     gene            complement(768..1235)
                     /locus_tag="VNN25_03460"
     CDS             complement(768..1235)
                     /locus_tag="VNN25_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90614.1"
                     /translation="MSEDRKYRHRGYQDSGGYSSNNSSSSSRPPRTPYEPQNQRIEGA
                     PRGRTAGGFGPEVFKCNACGTQRLGLDQPTSEETCIKCGADLHTCGNCRAFDTTTLWE
                     CRENIPARISGKHVRNECTFFQPKVVRDLTADKGRQVQTPDEARKAFDALFKK"
     gene            complement(1242..1394)
                     /locus_tag="VNN25_03465"
     CDS             complement(1242..1394)
                     /locus_tag="VNN25_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90615.1"
                     /translation="MPSMNELIGLLIAIIVIWFVLKLAKVAIRLILFVIAVVIIAGAL
                     YYVFMR"
     gene            complement(1491..2588)
                     /locus_tag="VNN25_03470"
     CDS             complement(1491..2588)
                     /locus_tag="VNN25_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90616.1"
                     /translation="MRSSRVLIATALFFVFIAPSFGTCTFPVTNTGLAGGGIRLSWQP
                     TASATNYEVQVSHDGFQHSTTLANLPAGTTGVTVNELTNSPKPYSYRIIASNPSNATD
                     VSCTGAATILYNTSFSGIYQRVSRIVIPVAGSTRGANNAQFKTSLRLGPPTSESAGKI
                     IFHPAGRGGSDNDPSIPYRVERGQTLQFDDIVAAIGQSGIGSIDVVPGNWFPLSDVGL
                     LPVEARLFNEAAEGTYGAFEAPVMPPDAYRPIDWNIFVPSARFRVNIGIRTITDAHVS
                     FSHFAAGGPVTEKVLDLPADYVFLDAADHFFGVAVNPGDSILISVSGDNVIAIPFHTF
                     TDNSTNDPAVFNPQSPAHVTFPILDIPTATP"
     gene            complement(2748..3863)
                     /locus_tag="VNN25_03475"
     CDS             complement(2748..3863)
                     /locus_tag="VNN25_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90617.1"
                     /translation="MRSSRVLIATALFFVVIGPSFGSCTFPVNISNALPGFGGYRLSW
                     QPVISATNYQVEASSDGFRHSTTLANLGPGTSGVTVNELTSYVRGSYSFRITATSPSN
                     PFASDVPCTGTAMNLNFSGIAISLARRVNRIVIPVAGSTRGANNAQFKTSLRLGPPTS
                     ESIGKIIFHPAGRAGSDDDPSIPFRVERGETLQFDDIVAAIGQSGIGSLDITFIGDFG
                     LPEVGLLPVEARLFNEGVGGTYGAFEAPVMPPDAYQPLDWNIFVPSARFRVNLGIRTL
                     TTAHVTFYHFAAGGPVTEKVLDLPADYVFLDAADHFFGVAVNPGDSIQISVSGDNVIA
                     IPFHTFTDNSTNDPAVFNPQSPAHVTFPILDIPTATP"
     gene            complement(4023..>4393)
                     /locus_tag="VNN25_03480"
     CDS             complement(4023..>4393)
                     /locus_tag="VNN25_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90618.1"
                     /translation="PDAYRPIDWNIFVPSTRFRVNLGIRTLTAAHVTFYHFAAGGPVT
                     EKVLDLPADYVFLDAADHFFGVAVNPVDSIQISVSGDNVIAIPFHTFTDNSTNDPAVF
                     NPQSPAHTYFPILDIATATP"
BASE COUNT          911 a         1193 c         1476 g          813 t
ORIGIN      
        1 cgaatgaccc agggaaccat gagcagccag gcgacgccgt gcgagagcca gccgagtgcg
       61 aacgcccctt tgtaactgtt cacgcgcgtt aacgcgacga acagtggcag cagcgcgatg
      121 aacatcagcc atccggcagc gacgttcggg aaggaccagg cgaagaggac gccggagacg
      181 atcgcgagag ctacgttgat tgcgtttcgc aagcgggcgg aggatagcag ttgcggagtc
      241 acgaggttta tgaggtcgcg gagcagaaaa aacgggacag ctttcggtga ccctgctact
      301 tgtgctgatg cggccggcgg tcgtagaaga tgttctggtc actatccaga acaatggtcg
      361 gcggcgttcc ttcggtgaag acgatgtagt gcaatttgcg atcgccgagt ggctgaaggc
      421 tcgtgattcc cgcatagtct tgccaatcgt tgatgacgac atgtgtccca ttcacgcgcc
      481 atgtgccagt gctcgagcgg tgtgcctgac cggaaacaaa actgtgttcg taggcgccgt
      541 cagccttcaa gagcaggcgg tcatcaggtt ggttcgggtt ggtgtagtcg gagttgacgt
      601 acaggtaata ctggccaacg agctcgcgtg tcgtcagcgg ctcgcggcgc ccgcaagcaa
      661 tcgcgagcag cagcacgatg agaatcttgc ttctatctgc gaacatctgc gtcatctgcg
      721 gaaaagcctt atccgcagat tacgcagata gacgcagatg aataccacta cttcttgaac
      781 agcgcgtcga acgccttccg cgcttcgtcg ggcgtttgca cctggcggcc cttgtcggcg
      841 gtgaggtcgc ggacgacttt cggctggaag aacgtgcact cgttgcggac gtgcttgccg
      901 gagatgcgcg cggggatgtt ctcgcggcat tcccagagtg tggtcgtgtc gaaagcgcgg
      961 cagttgccgc atgtgtgcag gtcggcgccg cacttgatgc acgtctcttc cgaggtcggt
     1021 tggtcgagtc cgaggcgctg cgtgccacag gcgttgcatt tgaagacttc ggggccgaaa
     1081 ccgccggcgg tacggccgcg gggagcgcct tcgatcctct ggttctgagg ctcgtagggt
     1141 gtgcgcggcg ggcgcgaact gctggatgaa ttgttagagg agtagccgcc gctgtcctga
     1201 tagccgcggt gtctgtactt gcgatcttcg ctcaaaggaa atcacctcat gaaaacgtaa
     1261 taaagcgccc cggcgatgat gacgacggcg atcacgaaga ggatcagacg gatcgccact
     1321 ttcgccagct tgaggacgaa ccagatcacg atgattgcga tcaggagccc gatgagctcg
     1381 ttcatgctgg gcatggtggg gtgtgtagtg taacaggagg cgtgcgactt tgattgcacg
     1441 agctcgcgat cgaggcaccg gcgcggcagc cggtgcctca cgcaagagag ctagggcgtc
     1501 gcagtcggga tatcgagaat gggaaaggtg acgtgggccg gcgactgcgg attgaagacc
     1561 gccggatcgt tcgtcgagtt gtcggtgaac gtgtggaagg gaatggcgat gacgttgtca
     1621 ccggagacac tgatcaggat cgagtcgccg ggattgaccg ccacgccgaa gaaatgatcc
     1681 gccgcatcga ggaagacgta atcggcaggg agatcgagga ccttctccgt cacggggcct
     1741 ccggccgcaa agtgagagaa gctgacgtgc gcatcggtga tcgtgcggat gccgatgttg
     1801 acacggaacc gtgcggaggg gacgaagatg ttccagtcga tcggccggta ggcgtccggc
     1861 ggcatcaccg gcgcttcgaa cgcgccgtat gttccctcgg ctgcttcgtt gaacagccgt
     1921 gcttcgaccg gcagcaatcc cacatcggat agaggaaacc aattgcccgg aacgacgtcg
     1981 atcgacccga tgccgctctg gccgatcgcg gcaacgatgt cgtcgaactg cagcgtctgt
     2041 ccccgctcga cgcggtacgg aatcgacgga tcgttatcgc tgccgccacg tcctgccgga
     2101 tggaagatga tctttcctgc gctctccgac gtcggcggcc caaggcggag cgacgtcttg
     2161 aattgcgcat tgttcgcacc acgagtcgag ccggcgaccg gaatgacgat tctgctgacg
     2221 cgctgataga tcccgctgaa ggaggtgtta tagaggatag tcgctgcgcc ggtacacgaa
     2281 acgtccgttg cgttcgacgg gttcgacgcg atgatgcggt aggagtacgg tttgggggaa
     2341 ttcgtcagct cgttcacagt gacgccggtc gtaccagcag gaagattcgc cagcgtcgtg
     2401 gaatgctgaa acccgtcgtg cgacacctga acttcataat tcgtcgcgct ggcggttggc
     2461 tgccacgaca gtcggatgcc gccgccggcc agccccgtgt tcgtcactgg gaacgtacac
     2521 gtcccgaacg acggcgcaat gaacacaaag aacaaagcag tagcgatcag gactcttgat
     2581 gatctcatgt acctctctcc tatttgattg tgcccgcctg cgcatgaaga cacagctgcc
     2641 cgtgaggatc tccggcggga aactgcgaga tgggcgtcga gctgggacat ggaacgagct
     2701 ccgcgatcga gtcaccggcg tcagcgccgg tgcctcacga aagagcgcta gggcgtcgca
     2761 gtcgggatat cgagaatggg aaaggtgacg tgggccggcg actgcggatt gaagaccgcc
     2821 ggatcgttcg tcgagttgtc ggtgaacgtg tggaacggaa tggcgatgac gttgtcgccg
     2881 gagacactga tctggatcga gtcgccggga ttgaccgcca cgccgaagaa atgatccgcc
     2941 gcatcgagga agacgtaatc ggcagggaga tcgagaacct tctccgtcac ggggcctccg
     3001 gccgcgaagt gatagaaggt gacgtgcgcg gtggtgagag tgcggatgcc gaggttgaca
     3061 cggaaccgtg cggaggggac gaagatgttc cagtcgagcg gctggtaggc gtccggcggc
     3121 atcaccggcg cttcgaacgc gccgtatgtt cccccaaccc cttcgttgaa cagccgcgct
     3181 tcgaccggca acaggcccac ctcgggaaga ccgaagtcac cgataaaagt gatgtcgagg
     3241 gacccgatac cgctctggcc gatcgcggca acgatatcgt cgaactgcag cgtctctccg
     3301 cgctcgacac ggaacggaat cgacggatcg tcatcgctgc cggcacgccc tgccggatgg
     3361 aaaatgatct ttccgatgct ctccgacgtc ggcggcccaa ggcggagtga tgtcttgaat
     3421 tgcgcattgt tcgcaccacg agtcgagccg gcgaccggaa tgacgattct gttgacgcgc
     3481 cgggcgagac tgattgcgat gccggaaaag ttgaggttca tcgccgtgcc ggtacaggga
     3541 acgtctgatg cgaacgggtt cgaggggctc gttgcggtga tacggaagga gtaactcccg
     3601 cgcacgtagg acgtcagctc gttcaccgtg acgccactcg tcccgggccc aagattcgcg
     3661 agcgtcgtgg aatgtcgaaa accgtcggac gacgcctcta cctggtaatt cgtggcgctg
     3721 atgactggct gccacgacaa ccggtaaccg ccgaaaccgg gcagggcatt ggagatattc
     3781 accgggaatg tgcacgaccc gaacgacggc ccaatgacca caaagaacaa agcagtagcg
     3841 atcaggactc ttgatgatct catgtacctc tctcctgttt gattgtgccc ggctgcgcat
     3901 gaagacacag ctgcccgcga ggatttccgg cgggaaactg cgagatgggc gtcgcgctgg
     3961 gacatcgaac tagctccgcg atcgaggcac cggcgcggcc gccggtgcct cacgaaagag
     4021 agctagggcg tcgcggtcgc gatatcgaga atgggaaagt acgtgtgggc cggcgactgc
     4081 ggattgaaga ccgccggatc gttcgtcgag ttgtcggtga acgtgtggaa gggaatggcg
     4141 atgacgttgt caccggagac actgatctgg atcgagtcga cgggattgac cgccacgccg
     4201 aagaaatgat ccgccgcatc gaggaagacg taatcggcag ggagatcgag aaccttctcc
     4261 gtcacggggc ccccggccgc gaagtgatag aaggtgacgt gcgcggcggt gagagtgcgg
     4321 atgccgaggt tgacacggaa ccgtgtggag gggacgaaga tgttccagtc gatcggccgg
     4381 taggcgtccg gcg
//
LOCUS       DATLVW010000103         4718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_251448, whole genome shotgun sequence.
ACCESSION   DATLVW010000103 DATLVW010000000
VERSION     DATLVW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251448"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VNN25_03485"
     CDS             <1..234
                     /locus_tag="VNN25_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH90619.1"
                     /translation="DGAAVRLTSHEFRVLSYLMHHRGRVVSQAELTEHIYAQSFNRDS
                     NTVEVFIARLRRKLGSAVIETVRGLGYRIEERT"
     gene            231..1196
                     /locus_tag="VNN25_03490"
     CDS             231..1196
                     /locus_tag="VNN25_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH90620.1"
                     /translation="MKSLRSRLLIGSLLWTAGLLSISHMLFLLVLRWHAVFRIEPHMV
                     LLAGAALMLAGLLYLRGGVSRIEQLRTRLIDVREGRSDRVEGTYPSEVQPLVNDLNAL
                     LAEREEAVRRAVAKAGDLAHGLKTPLAVIAQEAARSSGAGVESGALIGQQVERMRRQI
                     DYHLAHARVGAAGTSAGIHCSVADSVDGLSRTLRRLHAERGIAIESNVSPDHAVRVRR
                     EDLDEMLGNLLDNACRFAKARVVVSSEQTEKLIAINIDDDGPGLPPSMRDEVLQRGVR
                     ADESEPGTGFGLAIVRDLAEVYGGSISLEGSPSGGLRARLQLPQG"
     gene            complement(1205..1444)
                     /locus_tag="VNN25_03495"
     CDS             complement(1205..1444)
                     /locus_tag="VNN25_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90621.1"
                     /translation="MSGPRLIVGIIVMGVAVPLTIFFLLGLHTVGEFFSVAATTFLAW
                     GTADFLSTILERPRLENRSPGLAIREDLDRRRADE"
     gene            complement(1478..2278)
                     /locus_tag="VNN25_03500"
     CDS             complement(1478..2278)
                     /locus_tag="VNN25_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH90622.1"
                     /translation="MSGLRKKYAGWALGASLALGGLGIPLKIGNMLHSADANAHRRTI
                     TGLTEKPQVDDAQPQTEAKIAGDLATAKQIADQVTSGIAGGVTGAVQDVATTVASAPA
                     TVAGAPSQIVAAADQVREQFFRKQIPFGGIIYSEARKNDLPPELVAAVVHTESSFVPT
                     ARSNRGAVGLMQLAPRTGRWLGADNLSDPAQNIQAGAKYLRYLTDRFGGDQQKAIAAY
                     NAGEGNVRRFNGTPPFKETRNYVQRVNNFRQDLGSRVDDETAATGAAQ"
     gene            2575..3192
                     /locus_tag="VNN25_03505"
     CDS             2575..3192
                     /locus_tag="VNN25_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXH90623.1"
                     /translation="MRPKLTIWLFAVAGALALTQSAHAQGYLCEESFKLDDATMQSYR
                     FGPPTPRPAPIAVAQPDPDPIKLRVLKYAAVALKHRLNGLASYYSTSLDGTLTANGER
                     YHNKKMSAAHLTLPLGSWIEVTSRATGKKLRLRVNDRGPYVNKFVIDLSQAAARYLGV
                     DVAEDRSVLIRIIGLPGEDPLPDDVIDGPKSTIIEETAALTPAGQ"
     gene            complement(3214..3924)
                     /locus_tag="VNN25_03510"
     CDS             complement(3214..3924)
                     /locus_tag="VNN25_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH90624.1"
                     /translation="MPVENNSDEVLTVEELTRLFSVLSHDLKSPIFSVDGFSDLLLAD
                     YSDKLDEEGQDFLRRIRSSAQQMKRVLDEMSHIVKLLSRPNARRPTPLREIVEEVILK
                     CNYQIEEGGVKVDLPDELPTVTVDPEKMREAISAIFANALFFSDRPKGERTIAIDCNV
                     DPDGYRICIKDNGIGIDPRYTAQIFDLGLKLDKSRGGGPGYGLYLAKRIIEDQGGTIS
                     VDALPDEGSSFCFTIPHS"
     gene            complement(3963..4646)
                     /locus_tag="VNN25_03515"
     CDS             complement(3963..4646)
                     /locus_tag="VNN25_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HXH90625.1"
                     /translation="MTTLHVDLIVPAQLIVLIGGGEFSFGETREIDEFLLRSMPPERR
                     VVAFIPAASGSAEYATHFGKYLHGIDPTVETVNVPIYRGRDSRRQKNLNALSSAGMIY
                     LGGGVTNNLLAVLRESAADLAMREAASQGTVIAAIGAAASCFGIAARDMHGIAPSLPA
                     LGWIANTVVDTSFEPENDTNLRRLMSVPETQLGLGIPVGTALAIRTGGETEIIGAGHV
                     AAFRKGQAR"
BASE COUNT          845 a         1459 c         1487 g          927 t
ORIGIN      
        1 gatggcgcag cggtccgcct gacaagtcac gaatttcgcg tgctttcgta cctgatgcat
       61 caccgcgggc gcgtggtctc gcaggcggag ctgacggagc acatttacgc gcagagtttc
      121 aatcgtgact cgaacaccgt cgaggtgttc atcgcccggc tgcggcggaa actcgggtcc
      181 gccgtcatcg agacggtgcg cggactcggc taccggatcg aggaacgcac gtgaaatcgc
      241 ttcgatcgcg attgctcatc ggctcgctgc tgtggacggc aggactactc agcatttcgc
      301 acatgctgtt tcttctcgtg ctccgatggc acgcggtctt ccggatcgag ccgcatatgg
      361 tcctcctcgc cggcgcggcg ttgatgctgg ccggacttct gtaccttcgc ggcggcgtgt
      421 cgcgcataga acaattgcgg acccggctca tcgacgtgcg cgaggggcga agcgatcgtg
      481 ttgaggggac gtatccatca gaagtacagc cgctggtcaa cgacctgaat gcgctactgg
      541 ccgagcgcga agaggcggta cgccgcgcgg ttgccaaggc gggcgacctc gcgcatggac
      601 tgaagacgcc gctcgccgtg atcgcgcagg aagcagcgcg cagttcagga gcgggcgtcg
      661 agtccggagc gctgatcggg cagcaggtcg aacggatgcg tcgccagatc gactatcacc
      721 tcgcacacgc acgcgttggc gccgcgggaa cgagtgccgg catccattgc tcagtggccg
      781 actcagttga cggactctcg cgcacgctgc gccgcttgca cgccgagcgc ggcatcgcga
      841 tcgagtcgaa cgtctctccc gatcacgccg ttcgtgtgcg ccgcgaagac ctcgacgaga
      901 tgctcggcaa cctgctcgac aacgcctgcc ggttcgcaaa agcgcgcgtc gtcgtctcat
      961 cagagcagac ggagaagctg atcgcgatca acattgatga cgacggcccc gggctgcctc
     1021 cttcgatgcg cgatgaggtt ttgcagcgag gcgtgcgcgc ggacgaatcc gagccgggta
     1081 ccggctttgg actggcgatc gtgcgcgatc ttgcggaagt ctatggaggc tcgatcagcc
     1141 tggaaggatc gccgtcgggc ggcctgcgtg cacggcttca acttccgcaa ggctgagtct
     1201 cgatctactc gtctgcgcgc cgccgatcga gatcctcccg aatcgccagc cccggtgaac
     1261 gattctcgag ccgcggcctt tccaggatcg tggagaggaa gtcggcagtt ccccaggcta
     1321 ggaacgtcgt tgcggcaacg ctgaagaact cgcccacagt gtggagaccg aggagaaaga
     1381 aaatcgtcag cggcaccgcg actcccatca cgatgatacc gacgatcaag cgaggcccgg
     1441 acatggccgt attgtatgcg cccgtgttgg tcgcgtccta ctgtgcagcg cctgtcgccg
     1501 cggtttcgtc gtccacgcgg gagcccagat cctgccggaa gttgttcact cgctgcacgt
     1561 agttgcgcgt ctctttgaac ggaggcgtcc cgttgaaacg gcggacgttc ccttccccgg
     1621 cgttgtacgc ggcgatcgct ttctgctggt cgccgccgaa gcgatcggtc aggtagcgaa
     1681 ggtactttgc tcccgcctgg atgttctgcg cggggtcgga aagattgtca gcacccaacc
     1741 agcgcccggt tcttggcgcg agttgcatca ggccgactgc gccgcggttc gaccgcgccg
     1801 tcggcacgaa actcgactcg gtgtggacga cagcagcgac gagctccggc ggcagatcgt
     1861 tctttcttgc ctcgctgtag atgatgccgc cgaagggaat ctgcttccga aagaactgct
     1921 ctctcacttg atcggcggca gccacgatct ggctgggcgc acccgccact gttgccggcg
     1981 ccgacgccac ggtcgtcgct acgtcctgga cggctccggt aacaccaccg gcgatgccgg
     2041 acgtgacctg gtcggcgatc tgtttcgccg tcgccagatc gccggcgatc ttcgcctcgg
     2101 tctggggctg cgcatcatcg acctgtggct tctcggtcag gccggtgatg gttcggcgat
     2161 gggcattcgc atcggcggaa tgcagcatgt ttccgatctt caaagggatc ccgagccctc
     2221 cgagggcgag cgaagcaccc agcgcccatc cggcatactt ctttcgtaaa ccgctcattc
     2281 tgcgcggttt gaccttcaat accgcgtcgt tgagttgggc gcggtcgaag ccatccagga
     2341 atctttcacg gaagggcctg cttgtgctgg gctcgctcat cgtagtagtc cccttttccg
     2401 tcccctctcg aaacggaatc ccggcttcgg ttatttgcaa gtgagaagcc acgacgagct
     2461 caatcgaaac aattcatcca gttgaagtta gctctgcaat agcgactttg tcttggcatg
     2521 gattggctcg cccattgaaa ggtagataaa atgacaagca gtaccgtatt ggctatgaga
     2581 ccgaagttga cgatctggtt gttcgctgtg gctggtgcct tggcacttac acagtcggcg
     2641 catgctcagg gctatctctg cgaggagagt ttcaagctcg acgatgccac gatgcagtcg
     2701 tatcgatttg gtccgccgac tccccgaccc gcgcccattg ccgttgcgca acctgacccc
     2761 gacccgatca aactccgcgt cctgaagtac gccgctgtcg cactgaagca ccgcctgaac
     2821 ggtctggcca gctactactc gacctcgctc gacggcacac tcaccgcgaa cggcgagcgc
     2881 taccacaata agaagatgtc ggcggcgcac ctgaccctgc cgctcggatc gtggatcgag
     2941 gtgacgtcgc gcgctacggg caagaagctc cgtctgcgcg tcaacgaccg cgggccgtac
     3001 gtgaacaagt tcgtcatcga tctctcccag gcagccgcgc gctacctcgg cgtcgacgtc
     3061 gcggaagacc gttccgtgct catccgcatc attgggcttc cgggggagga tcccttaccg
     3121 gacgatgtga tcgacgggcc gaagtcgacg atcatcgaag agaccgcggc tttgacgcca
     3181 gcaggccagt agcgaggacc cggcacaacc ggttcacgag tgcgggatcg tgaagcagaa
     3241 actgctgccc tcgtcaggta gtgcgtcgac gctgatcgtc ccgccctgat cttcgatgat
     3301 gcgtttggcg agatagaggc cgtagcccgg cccaccgccg cgtgacttgt cgagtttcag
     3361 gccgaggtcg aagatctgtg ccgtgtaacg cggatcgatg ccgatgccgt tgtctttgat
     3421 gcagattcgg tagccgtcag gatcgacgtt gcagtcaatg gcgatcgtgc gctccccttt
     3481 cgggcggtcg ctgaaaaaga gtgcgttcgc gaagatcgcg ctgatcgctt cgcgcatctt
     3541 ctccgggtcc acggtgaccg tcggcagctc gtcgggcaga tcgaccttca cgccgccttc
     3601 ttcgatctgg tagttgcact tgaggatgac ctcctccacg atttcccgca gcggcgttgg
     3661 gcgccgcgca ttgggacgcg acaacagctt gacgatgtgg gacatctcgt cgagcacccg
     3721 cttcatctgc tgcgcactgg agcggatgcg gcggaggaag tcctgccctt cctcatcgag
     3781 cttgtcggag taatcggcca gcagcagatc gctgaacccg tcaaccgaga agatcggcga
     3841 cttgaggtcg tgcgacagca cgctgaagag gcgcgtcagc tcttctacgg ttagaacttc
     3901 gtcggaattg ttctctactg gcatcgactg ctccgggcct ggcagcacgc gcatttctgc
     3961 aactagcggg cctgaccttt ccgaaaagcg gcgacatgcc ccgcgccgat gatctccgtc
     4021 tcgccgccgg tgcgaatcgc cagggcggtg cccacgggga tgccgagtcc gagctgcgtt
     4081 tcgggaaccg acatcagacg ccgcaagttc gtgtcgttct cgggctcgaa ggaggtgtcg
     4141 accacggtgt tcgcaatcca gccaagggcg ggaagcgagg gggcgatgcc gtgcatgtcg
     4201 cgcgcggcga ttccgaagca cgaggctgcc gctccgattg ccgcgatcac ggtgccttgc
     4261 gacgccgcct cgcgcatcgc caggtcggca gccgattccc gcagcacggc aagcaggttg
     4321 ttcgtcacgc cgccgccgag gtagatcatg ccggctgagg agagcgcgtt gagattcttc
     4381 tgccgccggc tgtcgcgtcc gcgatagatg ggaacattca cggtttcgac agtcggatcg
     4441 attccatgca ggtactttcc gaagtgcgtc gcgtactcag ccgatcccga cgccgccggg
     4501 atgaacgcaa cgacacgccg ctccgggggc atgctgcgga ggagaaactc atcaatctcg
     4561 cgggtctcgc caaaagaaaa ctcaccgccg ccgatgagga cgatgagttg cgcggggacg
     4621 atgaggtcaa cgtggagggt ggtcacgggc ggtttttacc acagagactc agaggtcaca
     4681 gaggtacggc tttgtctctg tgtcctcagt gtctcttt
//
LOCUS       DATLVW010000104        12748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_337106, whole genome shotgun sequence.
ACCESSION   DATLVW010000104 DATLVW010000000
VERSION     DATLVW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337106"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VNN25_03520"
     CDS             complement(<1..682)
                     /locus_tag="VNN25_03520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXH90626.1"
                     /translation="MRTLILAFAICASIIVPLLLLLLFPPWWLIALGVLALAHGFVLW
                     PTLRPNSQWLGPVMTSFETDSNEVWLTIDDGPTDDTPALLDALDALDVKATFFVKGVL
                     VEQRPDLVKAMLARGHTVENHSQTHPSGSFWCLPRKALAWQIDRCRKALETVGVQSRL
                     FRAPVGMKNPFVHPILQRRDLTLVGWSVRGFDSFGDDVQRVVKRIVPRVTSGSIVVMH
                     QGRGFSVAC"
     gene            complement(670..1251)
                     /locus_tag="VNN25_03525"
     CDS             complement(670..1251)
                     /locus_tag="VNN25_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90627.1"
                     /translation="MVHVRAVLAGAGRVTMNVIVNRSAARWLPSRHRFALAQLVAFTH
                     LRQGKPQDANGPDHLILHIDRDQSAAEAYAVAVWVFVTVAAYLAAILPVPQPLAIIVA
                     IPLASIVIHIPIVVGGLLLRLILGDNDHVKIVSVGTMSLLFVWSFYVARGDSWARFVA
                     WFFFAVLACNAIAAAILWLLRAAVQAEESRCAR"
     gene            complement(1209..2108)
                     /locus_tag="VNN25_03530"
     CDS             complement(1209..2108)
                     /locus_tag="VNN25_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXH90628.1"
                     /translation="MTQPSWWHRFSVRGVFWRQLLRFAILNVPPWIEPIVMASWSLFF
                     LMWGSARRAVIRNLGVIKPGSFAIVNFFRCYRLFWNYAWTIADNVRFKEARTIPDWEF
                     TGWKFFQEMQERDGGAILLTAHMGSYDLGAHLFAETSSRRIVMVRAPEADPETRAFEQ
                     DRAAEGLRIEFNTRSTDLAIDLLDVIREGNIVAIQGDRVTGEIGFLPATVFGKPTRVP
                     AGPFALALAARVPIFPVFVVRLGRRRYRLMTARPIELVRSRDRDDDFRRAVEQWVEEL
                     EHVIRDCWSQWFTFEPYWPELAA"
     gene            complement(2105..3376)
                     /locus_tag="VNN25_03535"
     CDS             complement(2105..3376)
                     /locus_tag="VNN25_03535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH90629.1"
                     /translation="MRGDSASARTRSFVSRPDQAPHGRLVLTAPAVDVIVVNWNSRAD
                     TMACLEAVASQLAGVEGAVITVVDNGSTDGSIGAIAALRPAVRLLPLGANHGFTGGVA
                     AGLARSDARNVVFLNNDAVPESGWLKALVDAIDAAPDDVISIGGKIVDPTGQFIDFIG
                     GALTFDGHAFQNGFRYPLASVDEPKSGAEILFACGGNMISRREPLLALGGFDDDYFAY
                     LEDVDFGWRSWLAGYRVLFEPRAVARHKSSATSQRLGDFERGVLFERNALQTALKNYE
                     NLNDTAASLFFTFLSRLHHYASTRNDNADELVREPFSKTSKTSHGFLARVRKKLFGAK
                     PLATIEDPLTTMQFRAFTWTVAHGERIAAKRAAVQNLRKRSDGEIFERFPLLVVPTYP
                     GDESLMRSALFRMLQPSLPSVERTLSAIMRP"
     gene            complement(3295..4527)
                     /locus_tag="VNN25_03540"
     CDS             complement(3295..4527)
                     /locus_tag="VNN25_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90630.1"
                     /translation="MTRALLVCPEPLGHRQPAGIGIRFIEMSKVLLADGHDVTLLSRD
                     AGNVAGCRVDAITPENLERYSHEAEVAIVQGHAANEVFAHAAPIPTVVDLYDPFIVEN
                     LHYYSSRGAEVFAHDHATLIQSLLRGDLFLCASEAQRLFYLGTLLATGRVNPIAFESD
                     PNLDALLRIAPFGVEAPGSAGVSSARGSDFPLPGVLFGAIYDWYDPILAIEAAAIARA
                     SLPDITLTFTTHPNPSLTPQGKLAEAMKVVEQKRYEFVRFEPWVAYEERALFFSRFGA
                     ALMTFPRSIETDLSMRTRVYDYIWGGLPIVTSSAPSTDELLTRYGAGTIVPSNFPAGL
                     AAALVRVLRDDHMRMREGARRFVEEHQWDRALQPLVEFVRSPRIDPSKDAFAATLRLP
                     EHVPSFLDRIKRRMGGSF"
     gene            complement(4524..6386)
                     /locus_tag="VNN25_03545"
     CDS             complement(4524..6386)
                     /locus_tag="VNN25_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXH90631.1"
                     /translation="MTIVEAEAIPFEPQDLRGERLLVLAPHPDDEVIGCGGLVALHLR
                     EGRPVHVVIATDGAQAGDGVQREAESRTALALLGDATIEFLRFPDRELENAAELDARL
                     AAILRQQTPDLIAVPSPLEIHPDHIALSRVFCDLIARDPSLFADLAVARIAFYEVSAP
                     LRPNALIDITSVAGTKYAAIDAHASQAAIRNYTSYARGLNAWRAMTLPTHVAFAEAYW
                     TTTLPSLRTTPFSALREAIGPQRIDIVREPLPVSVVVRTKNRPALLAEAIASIRATGY
                     PAEIVVVNDGGTPPEVENVTLVQHDTSLGRSEAANAGVRAAKSDYVAFLDDDDLFYPE
                     HLSTLSHAARGSSAAAWYSDAVSAFIDGESRQPMRIYARDFDRELLLVDNYIPLPTLL
                     LRRANFLDVGGFDAAFDLFEDWDFLIRLAQRGDFIRVPRITCEVRQIKNSGSIVAANP
                     EGSPTFRKAKLQVWRKHAALMSESMFANVFERERRRTVGIEGELVEARGARSRADCEV
                     ARLERDKRSLIAQIGALSERINETMMRISHLEGANAEIRGMLDSANRDCYAAVARISE
                     LERAAAARETASDEIERTNRALYAEVARQQNLLDTIFKSRTWKLHSIVERVKGR"
     gene            6621..7637
                     /locus_tag="VNN25_03550"
     CDS             6621..7637
                     /locus_tag="VNN25_03550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH90632.1"
                     /translation="MRRDQQQPIAGVLSLIRELTGSATRADNITDLFVSSFRALDHAI
                     DFDLAAAVMVEQNLELYVVTREGAASLIGPPLIDSIRRTLETMIPVSFAASDVIVKAE
                     RSDLPRAGGGDSLEHSTHAVITVDNHSSGILLVYRSEPFDEEEQQIVEIFAAQVSLLI
                     GQLSAREQIQGLADTDDLTGIANKRYFRRQLPQEIERARVYSLPLALLMFDVDDFKEI
                     NDGFGHTIGDVVLSELCGAVRETLRPPDLFARFGGDEFAIILPHTDLDGACSVANRIL
                     NRVRELTIPTDDESMIQCSISIGIASYRSSDKAAGDVIRRADEQLYEAKREGKNRYAA
                     ASAS"
     gene            7658..8419
                     /gene="tpiA"
                     /locus_tag="VNN25_03555"
     CDS             7658..8419
                     /gene="tpiA"
                     /locus_tag="VNN25_03555"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXH90633.1"
                     /translation="MPNRYLIANWKMNLPPEGIGAFLGAVNAEGIVVAPPFPYLREVA
                     AGASKRVAVAAQNCADQAAGAFTGEVSARMIRDCGARFVILGHSERRNIYGESDTVVA
                     KKTALAIENGLTPIVCIGENLNERERGNAMTVCSSQIAALAIPQLANAGEVIVAYEPV
                     WAIGTGRNATSAMVAEMARAVRDALVRYWPADLAQRAPVLYGGSVTPENAGDLVAHGG
                     IDGFLVGGASLDSKKFLSLAESMNLLSRSQNDVMI"
     gene            8550..8840
                     /gene="secG"
                     /locus_tag="VNN25_03560"
     CDS             8550..8840
                     /gene="secG"
                     /locus_tag="VNN25_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXH90634.1"
                     /translation="MTVFLVVLHVLISLFLILVVLLQQGKGADLAGAFGGGGSQTAFG
                     ARGATTLLHKLTTAFFVLFVITSLSLAILQARPRSSVMSGSAPSAPAAPAKK"
     gene            8857..8941
                     /locus_tag="VNN25_03565"
     tRNA            8857..8941
                     /locus_tag="VNN25_03565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8891..8893,aa:Leu,seq:gag)
     gene            complement(9105..11189)
                     /locus_tag="VNN25_03570"
     CDS             complement(9105..11189)
                     /locus_tag="VNN25_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH90635.1"
                     /translation="MGKKARNKRVAASVEAHAPTATAASADSRVALANPTIAGIAAAL
                     SVAVLAIFSQLRSHQFLTFDDPIYITNNSHVKAGVTMAGLRWAMTSLDFNWHPLTWFT
                     HMLDVQLFGLDAGDHLLVNVALHLANTLLVLAFLIIGTGRIWRSGFVAGLFAVHPLHV
                     ESVAWIAERKDVLCAFFFLLGIVLHCRFVQTRSRLLYAVVIVSFVLGLMSKGMIITLP
                     FVLLVVDYWPLQRWSLRDLRGLRPLILEKIPLFLLVIPAAVVTLAAQDHAKAITAHIG
                     LTERLANAAISYVEYLVKTIWPADLAAFYPYRVVIAPLLAFGAILVLIAITAAVMQQA
                     ARRPYLPAGWFWFLGILVPVIGIVQIGRQSMADRYTYLPLIGIFIAITWLLADLVPSK
                     VTIGAVGVTGILVLSILAHRQAGYWQDSIGLFSHALEVTPDNATAETLLGQAFLESAD
                     YTNAGKHFAVAAAMSPNDARVHNSLGLALSGLGDAGGAVREWKAAIAADAAFAEPYSN
                     LGRLELGAGRKKEAEGWFEQSIQRDPAPRTFAELARARGDLDEAVRRYQQAIAADSQS
                     SEIHNDYAATLAIAGRDQQAREEYETALRLSPGNYDADMNLGALLSRMGQNVAAVDHF
                     RRAAQERPQSFEPHVYLALVLAQTGNRDAAIGEMQKAMAIDATSTNRQVLKATGGQFD
                     GQVFIAGLQGQR"
     gene            complement(11437..11757)
                     /locus_tag="VNN25_03575"
     CDS             complement(11437..11757)
                     /locus_tag="VNN25_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90636.1"
                     /translation="MTEEPLKDQRTQILSGVVATLLEDLKSGAGDKDRRRQVEEWMRT
                     LAEKYPEFHIESGLRDYYLAEAERLRADFEKAAELTEKLALGRSIEGFLDRAADYAKR
                     IAEK"
     gene            11795..>12748
                     /locus_tag="VNN25_03580"
     CDS             11795..>12748
                     /locus_tag="VNN25_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXH90637.1"
                     /translation="MKVLVANRGEIAVRVMRACREMGYPMVAVYSEPDRAALHVLYAD
                     EAMPIGPAPSRESYLRIDRILDAAKRTGAEAVHPGYGFLAENAAFARACHDAGILFIG
                     PSPESIEAMGSKTESRQRMKAAGVPVVPGLTEAVKSFDEIEAFARDAGFPIMIKASAG
                     GGGKGMRLVEREGDLRTAFERVTSEAQSFFGDGAVYAEKFIASPRHIEVQILGDQHGN
                     VVYIGERECTLQRRHQKVVEECPSPVVDQALRERLGAMAVKAASAVNYYSAGTIECLM
                     GPDKQFYFLEMNTRLQVEHPVTEMVWGLDLVKWQLRIAQGEK"
BASE COUNT         2477 a         3896 c         4119 g         2256 t
ORIGIN      
        1 cgcacgccac cgaaaagccg cgcccctgat gcatcaccac gatcgagccg gaagtgacgc
       61 gcggcacgat tcgcttcaca acgcgctgca cgtcgtcgcc gaaggaatcg aatccgcgca
      121 cgctccaacc gacgagcgtc aggtcgcggc gctgcaggat cggatggacg aacgggttct
      181 tcattccgac cggcgccctg aaaaggcgtg actgcacacc tactgtttcc agcgcttttc
      241 tgcatcgatc gatctgccaa gccagtgcct tccgcggaag gcaccagaac gagcctgaag
      301 gatgcgtctg cgagtggttc tcgacggtgt gaccgcgcgc cagcatcgcc ttcacaaggt
      361 cgggccgttg ttcgacgagg acacctttga cgaagaacgt cgcctttacg tcgagcgcgt
      421 cgagcgcgtc gagcagcgct ggcgtgtcgt ccgtgggccc gtcgtcaatt gtgagccaga
      481 cttcgttgga gtcggtctcg aaactcgtca tcaccggacc gagccactgc gagttcggtc
      541 taagtgttgg ccagagcacg aacccatgag cgagagcgag aactccgagg gcgatcagcc
      601 accacggcgg aaacagcagc aggagaagga gcgggacaat gatcgatgcg cagattgcaa
      661 acgcgaggat cagcgtgcgc atcgactctc ctcggcctgg accgcggcgc gcagcaacca
      721 gagaatcgcc gcggcgatcg cattgcaggc caacacggca aagaagaacc aagccacgaa
      781 gcgcgcccag gaatcgccgc gcgccacgta aaacgaccag acgaacagca aggacatcgt
      841 tccgacggag acaatcttga cgtgatcgtt gtctccgaga atgagccgga gaagcagtcc
      901 tccgacaacg attgggatgt gaatgacgat cgatgcaaga ggaatcgcca cgatgatggc
      961 cagaggctgg ggcaccggca ggatcgctgc gagataggcg gcgacggtga cgaaaaccca
     1021 gaccgcaacg gcataagctt cggccgcgct ttgatcgcga tcgatgtgaa ggatgaggtg
     1081 gtccggacca ttggcgtctt gcggcttgcc ttgccgcaag tgtgtgaacg caacaagctg
     1141 cgccagcgca aagcgatgcc gcgacggcag ccatcgagcg gcggaacgat tgacgatgac
     1201 gttcatcgtc acgcggccag ctccggccag tacggctcga acgtgaacca ctgagaccag
     1261 cagtcgcgga tgacatgctc gagctcttcc acccattgct cgaccgcgcg ccggaagtcg
     1321 tcatcgcggt cccggctgcg tacgagctcg atcgggcgcg cggtcatgag tcgatagcgg
     1381 cgcctgccga gtcggacgac gaagacggga aagatcggca cgcgcgccgc gagcgccagc
     1441 gcaaacgggc cggccggaac gcgcgtcggc ttaccgaaca ccgtcgcggg gaggaagccg
     1501 atctcgccgg tcacgcggtc gccctggatg gccacgatgt tgccttcgcg gatgacatcg
     1561 agcaggtcga tggcgaggtc agtggatcgt gtattgaact cgatgcgaag accttcagcg
     1621 gcgcggtcct gttcaaaggc tcgcgtttcc ggatcggctt cgggggcgcg caccatgacg
     1681 atgcgccggc tcgatgtctc ggcgaagaga tgtgcgccga ggtcgtagct gcccatgtgc
     1741 gcggtgagca ggatcgcgcc gccgtcgcgc tcctgcatct cttgaaaaaa cttccagccg
     1801 gtgaactccc aatccggaat cgtgcgagcc tccttgaacc ggacgttgtc ggcgatcgtc
     1861 caggcgtagt tccagaagag gcggtagcag cggaagaaat tgacgatggc gaacgagcct
     1921 ggcttgatca cgccgagatt gcggatcaca gcgcggcggg ccgatcccca catcaggaag
     1981 aacaacgacc atgacgccat gacgatcggc tcgatccagg gcggtacgtt cagaatggca
     2041 aagcggagaa gttgccgcca gaacacgcct cgcacgctaa aacgatgcca ccaggacggc
     2101 tgtgtcacgg gcgcatgata gcggacagcg tccgctcgac tgacggcaaa gagggctgga
     2161 gcattcggaa gagcgcactg cgcatcagac tctcgtcgcc gggataggtt ggaacgacca
     2221 gcaggggaaa gcgctcgaag atctcgccgt cgctgcgctt ccgcaggttc tgcacggcgg
     2281 cgcgtttcgc ggcgatgcgt tctccatgag ccacggtcca agtgaaggca cggaactgca
     2341 tcgtggtgag cggatcttcg atcgttgcca acggcttcgc accgaacagt ttcttgcgta
     2401 cgcgcgccag aaatccgtgc gaagtcttcg acgtcttcga gaagggctcc cgaaccaact
     2461 cgtccgcgtt gtcgttgcgg gtcgacgcgt agtgatggag gcgcgataag aaggtgaaga
     2521 acaacgaggc tgcggtgtcg ttgaggttct cgtagttctt cagcgcggtc tgcaacgcat
     2581 tgcgctcgaa gagaacgcct cgctcgaaat cgccgaggcg ctggctcgtc gcgctcgact
     2641 tgtgacgcgc caccgcgcgg ggctcgaaca gaacgcggta accggccagc caggagcgcc
     2701 agccgaaatc gacatcttcg agatacgcga agtagtcgtc gtcgaatccg ccgagggcga
     2761 ggagcggctc gcggcgcgag atcatgttcc caccgcaggc gaagagaatc tccgcgccgg
     2821 acttcggttc gtcaaccgag gcgagtggat aacggaatcc gttctggaag gcatgaccat
     2881 cgaacgtcag cgctccgccg atgaaatcga tgaattgccc ggtcggatcg acgatcttgc
     2941 cgccgatcga gatcacgtcg tccggcgctg catcgatcgc gtcgacgagc gccttcagcc
     3001 atcctgactc cggcaccgca tcgttgttga gaaagacgac gttgcgcgca tccgagcggg
     3061 ccagacctgc ggcgacgccg cctgtgaatc cgtggtttgc gccgagcggc aggagacgca
     3121 ccgccgggcg gagtgcggcg atcgcgccga tgctgccatc ggtcgagccg ttgtcgacga
     3181 ccgtgatcac cgcgccctca acgccggcaa gctgcgatgc gaccgcctcg aggcaggcca
     3241 tcgtatccgc gcggctgttc caattcacga cgatgacgtc aacagcgggt gcggtcagaa
     3301 cgagcctccc atgcggcgct tgatccggtc gagaaacgaa ggaacgtgtt cgggcagacg
     3361 cagagtcgcc gcgaatgcgt ctttggacgg atcgatgcgc ggtgagcgaa cgaactcgac
     3421 gagcggctgc aacgcgcgat cccattgatg ctcttcgacg aagcgtctcg cgccctcgcg
     3481 catgcgcatg tggtcatcgc gcaacacgcg cacgagcgcg gccgcgagcc cggcagggaa
     3541 gttggagggg acgatcgttc ccgcgccgta tcgcgtcagc agttcgtccg tgcttggtgc
     3601 ggagctggtg acgatcggca gaccgcccca gatgtagtcg tagacgcgcg ttcgcatcga
     3661 gagatcggtc tcgatcgatc gcggaaaggt catcagcgcg gcgccgaagc gggagaagaa
     3721 caacgcgcgt tcttcataag caacccacgg ctcgaaacga acgaactcat acctcttctg
     3781 ctccacgacc ttcatcgcct cggccagttt tccctgcggc gtcagcgagg gattcggatg
     3841 cgttgtgaag gtcagggtga tgtccggcag cgacgcgcgc gcgatcgcgg ccgcttcgat
     3901 ggccaggatc gggtcatacc aatcgtagat cgccccgaag agcactcctg ggagagggaa
     3961 atcagaacca cgagcggacg agacgcccgc gctcccaggg gcttctacac caaagggggc
     4021 gatgcgcagg agcgcgtcga ggttcggatc gctctcgaac gcgatcgggt tgacgcgtcc
     4081 ggtggcgaga agtgtgccga ggtagaagag gcgctgcgct tcggacgcgc agagaaagag
     4141 atcgccgcgc agcagcgact ggatcagcgt ggcgtgatcg tgcgcgaaca cctcggctcc
     4201 gcgcgacgag tagtagtgca ggttctcgac gatgaacggg tcatacagat cgacgaccgt
     4261 cgggatcggt gcagcgtgcg cgaacacttc attcgccgca tgtccctgca cgatggccac
     4321 ttctgcctcg tgcgagtatc gctccagatt ctcgggcgtg atcgcgtcga cgcggcagcc
     4381 ggcaacatta ccggcgtcgc gggagagaag tgtcacgtcg tgtccgtcgg cgagaaggac
     4441 tttggacatc tcgatgaaac ggatgccgat gcccgccggc tgccggtgac cgaggggctc
     4501 gggacagacg agcagcgcgc gcgtcatcgt cctttcaccc gctcgacgat tgaatgcagt
     4561 ttccaggtgc gcgatttgaa gatcgtgtcg agcaggttct gctgccgcgc cacctcggcg
     4621 tacagagccc ggttcgtgcg ctcgatctca tccgaggcag tttctctcgc ggccgccgct
     4681 ctttcgagct cgctgatccg ggcaacagcg gcgtaacagt cgcgatttgc gctatccagc
     4741 atgccgcgaa tctcagcgtt cgccccttcg aggtggctga tgcgcatcat cgtttcgttg
     4801 atccgctcgc tcagcgcgcc aatctgagcg atgagcgacc gcttgtcgcg ctcgagccgt
     4861 gcgacctcgc aatccgcgcg gctgcgcgcc ccacgtgctt cgaccagttc gccttcgatt
     4921 ccgaccgtgc ggcgccgctc ccgctcgaac acattcgcga acatgctctc gctcatcagc
     4981 gcggcgtgct tgcgccacac ctgcagcttg gcctttcgaa acgtgggaga accctcggga
     5041 tttgccgcca cgatcgatcc gctgttcttg atctgacgca cttcgcaggt gatgcgaggc
     5101 actcggatga aatcgccgcg ttgcgccagc cggatgagaa agtcccaatc ctcgaagagg
     5161 tcgaatgccg catcgaatcc accgacgtcg aggaaattgg cgcggcgaag caagagtgtg
     5221 ggaagcggga tgtagttgtc gacaaggagc agctctcgat cgaaatcgcg agcgtagatg
     5281 cgcattggtt gacggctttc accgtcgatg aaggcggaga cagcgtcgct ataccacgcc
     5341 gcggcgctcg atccgcgagc ggcatgcgac aacgtcgaga gatgctcggg gtagaagaga
     5401 tcatcgtcat cgaggaaggc gacatagtcg ctcttcgccg cgcgaacgcc ggcattggcg
     5461 gcttcggagc ggccgaggga cgtgtcgtgc tggacgagcg tgacgttctc gacctcaggc
     5521 ggcgtaccac catcgttcac gacgacgatc tctgccggat agccggtggc gcggatcgat
     5581 gcgatcgcct cggccagaag ggccggccga ttcttcgttc gcaccacgac gctgaccggc
     5641 agcggctcgc gcacgatgtc gatccgctgc ggacctatcg cttcgcgaag cgcgctgaag
     5701 ggcgtcgtac gaagcgacgg cagcgtcgtc gtccagtacg cttcggcgaa cgcgacatgc
     5761 gtgggcaggg tcatggcgcg ccacgcattg agcccgcggg catatgaagt gtaattgcgg
     5821 atcgcggcct gcgaagcgtg cgcgtcgatc gccgcgtact ttgtgccggc gacggacgtg
     5881 atgtcgatca gcgcattcgg acggagcggc gcgctgacct cgtagaacgc gatgcgggcc
     5941 accgcgaggt cggcgaagag cgacggatcg cgcgcgatga ggtcgcagaa gacgcgggaa
     6001 agagcgatgt gatcgggatg gatctcgagc gggctcggaa ctgcgatgag atcgggcgtt
     6061 tgctggcgca ggattgcggc gaggcgcgcg tcgagctcgg cggcattctc cagttcgcgg
     6121 tcggggaatc ggagaaactc gatcgtggca tcgccgagga gcgcgagtgc ggtgcgtgac
     6181 tccgcttcgc gctggacgcc atcgccggcc tgcgctccgt ccgttgctat gacgacgtgc
     6241 accggccgtc cctcgcgaag gtggagggcg accaggccgc cgcatccgat gacctcatcg
     6301 tcgggatgcg gtgccagcac caggaggcgc tcgccgcgta agtcctgcgg ctcgaatgga
     6361 atcgcctccg cctcaacgat ggtcatggct cggattatag gtggtctgtg aggggtgtcg
     6421 ggcgtcgggg gtcgggtgtc ggagcgctga tgcatcggct ggcggttgtt ccgccacttg
     6481 cccgcttgtc accggcacaa tgtccattgt caattctcta ttgaagcgaa gcgccctcgt
     6541 cgtccgtata atcatcaatt cccagaagcc agcaagactc tccgacaccc gacccccgat
     6601 acccgacacc cgacaacacc gtgagacgtg accaacagca gccgattgcc ggagttctgt
     6661 cgctgatccg cgagctgacc ggctccgcca cgcgcgctga caacattacg gatctcttcg
     6721 tctcttcgtt tcgtgccctc gatcacgcca tcgatttcga tctcgccgcg gcggtgatgg
     6781 tggagcagaa cctcgagctc tatgtcgtca cgcgggaagg ggccgcctcc ctcatcggac
     6841 ctccgctcat cgacagcatc cgccgcacgc tggagaccat gatccccgtc tccttcgccg
     6901 cctcggacgt catcgtgaaa gcggaacgga gcgatctgcc gcgcgccggc ggcggcgaca
     6961 gcctggagca ctcgacccac gcggtcatca ccgtcgacaa tcacagcagc ggcatcctgc
     7021 tggtctatcg gagcgagccg ttcgacgaag aggagcagca gatcgtcgag atcttcgccg
     7081 cgcaggtctc gcttctcatc ggccagctca gcgcccgcga acaaatccag ggtcttgccg
     7141 acaccgacga cctcaccggc atcgccaaca agcgctactt ccgccggcaa ctcccgcagg
     7201 agatcgagcg tgcgcgcgtc tattcgctgc cgctggcgct gctgatgttc gacgtcgacg
     7261 acttcaagga gatcaacgac ggattcggcc acacgatcgg cgacgtcgtg ctttcggagc
     7321 tctgcggcgc agtgcgcgag acgctccgtc cacccgacct tttcgcccgt ttcggcggcg
     7381 acgagttcgc gatcatcctt ccccatacgg acctggacgg cgcctgctcc gtggccaatc
     7441 gcatcctcaa tcgcgtgcgc gagctgacga ttccgaccga tgacgagagc atgatccaat
     7501 gctcgatctc gatcggcatc gccagctatc gttcctcgga caaggccgcc ggtgacgtga
     7561 ttcgccgcgc ggatgagcag ttgtacgaag cgaagcggga agggaagaac cggtatgccg
     7621 cggccagcgc ctcgtgactg gcgatagact caccaccatg ccgaaccgct atctcatcgc
     7681 caattggaaa atgaatctgc cccccgaagg gattggcgcc tttctcggcg cagtgaacgc
     7741 ggaggggatc gtcgtcgcgc ctccgtttcc ctatctccgc gaggttgccg caggtgcgtc
     7801 gaagagagtt gccgttgcgg cgcagaactg tgcggatcaa gcggctggcg cgtttaccgg
     7861 cgaagtttcg gcgcgcatga tccgcgattg cggcgcgcgg ttcgtgatcc tcggtcactc
     7921 ggagcggcgc aacatctatg gcgaaagcga cacggtcgtc gcgaagaaga ccgcgctggc
     7981 gatcgagaac ggtctgacgc cgatcgtgtg catcggcgaa aacctcaacg agcgtgaacg
     8041 cggcaacgcg atgacggtct gctccagtca gatcgccgca ctcgcaatac cgcagctcgc
     8101 aaatgcggga gaggtgatcg tcgcgtacga gccggtctgg gcgatcggca ccggacgcaa
     8161 cgccaccagc gcgatggtgg cggagatggc ccgcgccgtc cgcgatgcgc tggtgcgcta
     8221 ctggccggcc gatctggctc agcgcgcgcc ggtcctgtac ggtgggagcg tgacgccgga
     8281 gaacgccggc gacctcgtcg cgcacggcgg gattgacgga ttcctcgtcg gcggcgccag
     8341 cctcgattcg aagaagttcc tctcgctcgc ggagtcgatg aatctgcttt cgcgctcgca
     8401 gaacgacgtg atgatttgat ctcgcaggcg agggcttgct ctcgatgtat cgtgcgcggc
     8461 tcgtcctcgc ggagcagcct tgacacctgc tccctttccg ggaagaattc gcccccttgc
     8521 gccgttagcg ccatcgcttc ggagaatcca tgaccgtatt tcttgtcgta ttacacgtcc
     8581 tcatcagtct gttcctgatc ctcgtcgtcc ttctgcagca ggggaaggga gcggatctgg
     8641 caggtgcatt cggtggcggc ggctcccaga ccgcgttcgg cgcgcgcgga gcgaccacgc
     8701 ttctgcacaa gctgacgact gcgttcttcg tcctcttcgt catcacctca ttgtcgctgg
     8761 cgatcctgca agctcgcccg cgctcttccg taatgagtgg cagcgctccc tcggcgcccg
     8821 cagctccagc aaagaagtag tagccgttct tttcaagccc aagtggcgga attggtagac
     8881 gcgcacgttt gaggggcgtg tggagaaatc cgtgccggtt cgagtccggc cttgggcacc
     8941 aacggaaaag aagtcaggca ggcaaccaca gagacataga gaacacagag gggatctctg
     9001 tgacctctgt gcctctgtgg tgaccggttt tttcaaattg acccactacc acccgatcga
     9061 tcggcggtca gtaagcctca tcgcggcgga gcacatgggc gggcctagcg ttgcccctgc
     9121 agtcccgcga tgaagacctg cccgtcgaac tggccgcccg tggctttcaa cacctgacgg
     9181 ttcgtggacg tcgcgtcgat ggccattgcc ttctgcatct ccccgatcgc cgcgtcgcga
     9241 ttgccggtct gggcaagcac gagagcgaga tacacgtgcg gctcgaacga ttgaggccgt
     9301 tcctgggcgg cccgccggaa atggtcgacg gctgcaacgt tttgtcccat ccggctgagc
     9361 agcgcgccca gattcatgtc ggcgtcatag tttcccggtg agagccgcag cgccgtttca
     9421 tattcttcgc gggcctgctg atcccgtccc gcgatcgcca gggttgcggc gtaatcattg
     9481 tgaatttcgg aggattggga atcggcggcg atggcttgct gatagcggcg gactgcctcg
     9541 tcgaggtcgc cacgcgcgcg cgccaattcc gcaaatgtcc tcggcgcggg gtcgcgttgg
     9601 atcgattgtt cgaaccagcc ctccgcttcc ttcttcctgc ctgcgccaag ctcgaggcgg
     9661 cccaggttgc tatacggttc cgcgaacgcc gcatcggcag caatcgcagc cttccactcc
     9721 cggaccgctc ctccagcgtc acccaacccg ctgagcgcga ggcccaggct attgtgaacg
     9781 cgtgcgtcgt tggggctcat cgcggcggct acggcaaaat gctttcctgc attcgtgtaa
     9841 tcggcgctct cgagaaaggc ctggcccagg agggtttcgg ccgtcgcatt gtcgggtgtc
     9901 acctccagcg catgcgaaaa gaggccgatg ctgtcctgcc agtaaccggc ctgccggtgg
     9961 gcgaggattg acaggacgag gatgccggtc actccaacgg ccccaatcgt cactttggac
    10021 ggaaccaaat cggccagcag ccaggtgatg gcgatgaaga tcccgattag cgggaggtac
    10081 gtgtatcgat ccgccatcga ctgcctgcca atctgcacga tgccgatgac cggaacgaga
    10141 atgcccagaa accagaacca tcccgcggga agatagggcc ttcgtgccgc ttgctgcatg
    10201 accgcggcgg tgatggcgat gagaacgagg atggccccaa atgcaagcag tggcgcgatc
    10261 acgacgcgat aggggtagaa cgccgccaga tcagccggcc agattgtctt gacgaggtac
    10321 tcaacatagg agatggcagc attggcgagc cgctcggtga gcccgatgtg cgctgtgatt
    10381 gctttggcgt ggtcctgggc cgcgagagtc acgacggccg ccggaatgac gagcaggaag
    10441 aggggaatct tctccaggat gagcggcctc aaaccccgca agtcacgaag cgaccatcgt
    10501 tgcaacggcc agtaatcgac cacaagcagc acgaagggga gcgtgatgat catccctttc
    10561 gacatcagac ccaacacgaa cgaaacgatc acaactgcat agaggagccg cgatcgagtc
    10621 tgcacgaacc ggcaatgaag cacgatgccg agaagaaaaa agaacgcgca gagtacgtcc
    10681 tttctctcgg cgatccaggc taccgattcg acatgaagag gatgcacggc gaacagtccc
    10741 gccacgaatc cgctgcgcca gatgcggccc gttccgatga tgaggaacgc gagaaccagc
    10801 aacgtgttgg cgaggtggag agccacattg acgagcaggt gatcgccggc atcgaggccg
    10861 aacaactgca cgtccagcat gtgcgtgaac caggtgaggg gatgccagtt gaagtcgagc
    10921 gaggtcatcg cccaacgcag gcccgccatc gttacgcctg ccttcacgtg cgagttgttg
    10981 gtgatgtaga tcggatcgtc gaaggtgagg aactgatgcg atcggagttg ggagaagatc
    11041 gcgagcacag ctaccgaaag agctgctgca attcctgcga ttgtgggatt ggcaagggcg
    11101 actcggctgt ctgcgctagc cgcggtggca gtgggtgcgt gtgcctcgac cgatgctgcg
    11161 acccgcttgt ttcgcgcttt cttacccatg ggccgggtag tgtagccgag ggtggtctgt
    11221 accagaggga cgcagagatc agaaggttga gtagcagaga tcagagacaa atcagagaca
    11281 aaccagagac cagagatcag agactggatc agtgaccaga gactggatca gagatcagag
    11341 atcagagact ggatcagaga ctggagatca gaacggtacc ctgatctcta gtctcttatc
    11401 gctgttctct ggtctcaacc gctcgcagca agcgccttac ttctccgcga tccgcttcgc
    11461 gtaatccgcc gcgcgatcga ggaatccttc gatcgatctt cccagcgcca gcttctcggt
    11521 gagctcggcc gctttctcga aatcggcgcg aaggcgttcc gcttcggcga ggtagtagtc
    11581 gcgcaggccg ctctcgatgt ggaactcggg gtacttctcg gccagcgtgc gcatccactc
    11641 ctcgacctgc cgacggcggt ccttgtctcc tgcaccgctc ttgaggtctt ccagcagcgt
    11701 ggcaacgaca ccggaaagga tctgtgtgcg ctggtctttg agaggttctt ccgtcataga
    11761 gcggggatgt tagcagcgaa tacaatgcgc ggcgatgaaa gtcctggtgg cgaaccgagg
    11821 agagatcgcg gtgcgggtca tgagagcgtg ccgggagatg ggctatccga tggtggccgt
    11881 ctactccgag cctgaccgcg ccgcgctgca cgtcctctac gcggacgaag ccatgccgat
    11941 cggtcctgcg ccatcgcggg agagctacct gcgcatcgac cgcattctcg atgccgcgaa
    12001 aaggactggc gccgaggccg tccatccagg ctacggcttc ctcgcagaaa acgcggcgtt
    12061 tgcacgcgcg tgccacgatg ccggcatcct gttcatcgga ccgtcgccgg agtcgatcga
    12121 ggcgatgggc tcgaagaccg agagccggca acggatgaaa gctgcgggcg tgccggtcgt
    12181 tcccggcctt accgaagcgg tgaagtcatt cgatgagatc gaagcctttg cccgcgacgc
    12241 cggctttccg atcatgatca aagcgtcggc gggcgggggc ggcaaaggga tgcggctcgt
    12301 cgagcgcgaa ggcgatttgc gcactgcctt cgagcgcgtc accagcgagg cgcagtcgtt
    12361 ctttggcgac ggcgccgtct acgccgagaa gttcatcgcc tcaccgcgcc acatcgaagt
    12421 ccagatcctc ggcgatcagc acggcaacgt cgtctacatc ggcgagcgcg aatgcacgct
    12481 gcaacgccgg catcagaagg tagtcgagga gtgtccgtct cccgtcgtcg atcaggcctt
    12541 gcgcgagcgc ctcggcgcga tggcggtgaa ggcagcgtcg gccgtcaact actactccgc
    12601 gggaaccatc gagtgtctga tgggacccga caagcagttc tacttcctcg agatgaacac
    12661 gcgtctgcag gtcgagcacc cggtcacgga gatggtgtgg ggcctcgacc tcgtgaagtg
    12721 gcagctccga atcgcgcagg gggagaag
//
LOCUS       DATLVW010000105         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_170832, whole genome shotgun sequence.
ACCESSION   DATLVW010000105 DATLVW010000000
VERSION     DATLVW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170832"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1017
                     /locus_tag="VNN25_03585"
     CDS             310..1017
                     /locus_tag="VNN25_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90638.1"
                     /translation="MHRIVRFVFAVVLASAALDAASQVTIVDNQFTHYAQGQMNSAAA
                     DNPNAYLYCWLYGNGLYCGAADGAGHYASCQTTDPAHKRLFAMIKRYSSVRFFWDPAD
                     SSCFLEVTVDSRNLRPRPVPATSKSSSPVFVDATAQYAYGSLAVKPDKPNQDIGCNEQ
                     SGFADLYCIAQDDAGNFVWCTTSKDSASQTLADMKLAVAGLNESSMLYFAWDNDPNTP
                     TRTCNFIYVFNASYLLP"
     gene            1035..1748
                     /locus_tag="VNN25_03590"
     CDS             1035..1748
                     /locus_tag="VNN25_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90639.1"
                     /translation="MRSIIRLAATLVLGAATTGAIADVAIVNFGTTGYFYGQLGAAAT
                     ASGPDAFIQCQLSGASLSCGASDQTGASARCSTTDAAVKNMFPMMDKSSIVQVFYDNA
                     GNCTKMAVVNLTRDLRLSSPAGSIKQSVPVSIDAANRIARGSISARPVSPNPDVTHES
                     INCTVRGNSVYECIAVDANSNSVHCLSFRSSAVQTLADQLLAAQAINEYSSVSFTYDA
                     QQLPECTSITVNNASYFGP"
     gene            complement(1733..2185)
                     /gene="dtd"
                     /locus_tag="VNN25_03595"
     CDS             complement(1733..2185)
                     /gene="dtd"
                     /locus_tag="VNN25_03595"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXH90640.1"
                     /translation="MIGLIQRVTGAKVAIGGEDVASIGKGVLALIGVERGDTEAEAER
                     LAARVLGYRIFPDASGKMNLSLRDIQGALLAVPQFTLVADTNSGTRPSFSSGASPEDG
                     RKMFLVFVEKAKALHSSVEAGRFGADMKVSLVNDGPVTFWLRVGQGPK"
     gene            complement(2182..2469)
                     /locus_tag="VNN25_03600"
     CDS             complement(2182..2469)
                     /locus_tag="VNN25_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXH90641.1"
                     /translation="MKSFTEGQHPDSARFRQERGIGMTEFPLSDEEKVQVRLHLHELE
                     MEHHDLDDIINRLSADPAQDQLQLRRLKKRKLVLKDQIQKLRARLIPNIIA"
     gene            2567..>3164
                     /locus_tag="VNN25_03605"
     CDS             2567..>3164
                     /locus_tag="VNN25_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXH90642.1"
                     /translation="MRRSDHDVTDSIRTTDPAAVGAEVARLFKDLYGKVSAEVVERGF
                     RDATALYRGEHADYYPCDTEYHDIQHVLDVTLAMARLMDGYERHRNDSPRLDPDVFEV
                     GIVAALFHDFGYLRRKRDRLHRYGAEYTLTHVSRGAEYLRGYVGELGLARHARTAAAL
                     VHFTGYERRVETIRLYDELLRRVGHMLGTADIIAQMSDR"
BASE COUNT          584 a         1088 c          922 g          570 t
ORIGIN      
        1 tattccgtga agcgcgcgcg ccgcacgcgg cgctgacgtc tgcgcgcacg cagttgtcat
       61 cttcgatgac aacatcacgc gctcgcgatt tttcccagcg atcacaggga ccaacgcttt
      121 cttccgcagt gcgcctgtag tcgtcattgc gtcccaacgc gagtgaattt tgtcacgcgc
      181 gcacgcgcca cattttcttc acgcaaggta ttcctcccac tgcgcgcggc gcgggaaaaa
      241 tatttctgca aaaaaattgc cgaacgcggc tagattctcg cgcacaagaa tttccccggg
      301 agacacaaca tgcatcgcat cgttcgtttc gtctttgccg tcgttctcgc cagcgccgcg
      361 ctcgatgcgg caagccaggt cacgatcgtc gacaaccagt tcactcatta cgcgcagggc
      421 cagatgaact cggccgccgc cgacaacccg aacgcatacc tctactgctg gctgtacggc
      481 aacggcctgt attgcggcgc cgcggacggg gccggccact acgcctcgtg ccagacgacc
      541 gatccggccc acaagcggtt attcgcgatg atcaagaggt actcgagcgt gaggttcttc
      601 tgggatccgg cggactcctc gtgcttcctc gaggtcaccg tggacagcag aaacctcagg
      661 ccgcggccgg ttcccgcgac ctccaagtca tcctcgccgg tcttcgtcga cgcgacggct
      721 cagtacgcgt acggctccct cgccgtcaag ccggacaagc ccaaccagga catcggctgc
      781 aacgagcaat cgggcttcgc cgacctgtac tgcatcgccc aggacgacgc gggtaacttc
      841 gtctggtgca cgacatccaa ggacagcgcc agccagacgc tggccgacat gaagctggcg
      901 gtcgccggtc tgaacgagtc ctcgatgctc tacttcgcct gggacaacga tccgaacacg
      961 ccgacccgca cctgcaactt catctacgtc ttcaacgctt cctaccttct gccgtaacga
     1021 gaggagcccc cgtcatgcgc tcaatcatcc gcctcgccgc gacgctcgtc cttggcgcgg
     1081 caacgaccgg cgcgatcgcc gatgtcgcga tcgtgaattt cggcacgacc ggatatttct
     1141 acggccagct cggcgcggcc gccaccgcga gcggcccgga cgcgttcatt cagtgccagc
     1201 tcagcggcgc gtcgttgtcc tgcggcgcca gcgaccagac cggcgcctcc gcgcgctgct
     1261 cgaccaccga cgcggcagtg aagaacatgt tcccgatgat ggacaagagc tcgatcgtcc
     1321 aggtcttcta cgacaacgcg ggaaactgca ccaagatggc cgtggtgaat ctgacgcgcg
     1381 atctgcggct gtcgtctccc gcggggagca tcaagcagtc cgtgcccgtc tccatcgacg
     1441 cggcaaaccg gatcgcgcgc ggctcgatca gtgcgcggcc ggtctctccc aatccggacg
     1501 tcacccacga atcgatcaac tgcacggtgc gcgggaactc cgtttacgag tgcattgcgg
     1561 tcgacgccaa cagcaactcc gtccactgcc tgtcgttccg aagcagcgct gtccagacgc
     1621 tcgccgacca gttgctggcg gcccaggcga tcaacgagta ctcgtccgtt tcgttcacct
     1681 acgacgccca gcaacttccg gaatgcacct cgatcaccgt caacaacgct tcctactttg
     1741 gaccctgacc aacccgcagc cagaacgtca ccgggccgtc gttcaccagg ctgactttca
     1801 tgtcggcgcc gaagcggccg gcctcgacgc tcgaatgcag tgctttcgct ttttcgacga
     1861 agacgagaaa catcttccgt ccgtcttcgg gtgatgcgcc gctcgagaaa ctcggccgcg
     1921 taccgctgtt cgtatccgcg accaaagtga attgaggcac agccaggaga gcaccttgaa
     1981 tgtcgcgtag ggaaagattc atcttccccg aagcatcggg gaagatccgg taaccaagaa
     2041 cccgcgccgc gaggcgctcc gcctccgctt cggtatcgcc acgctcgacg ccaatcagcg
     2101 cgaggacgcc tttgccgatc gaggcaacgt cttcgccacc gatggcgacc ttcgccccgg
     2161 tgacgcgctg aatcaaaccg atcaagcgat gatgttgggg atgaggcggg cgcgcagttt
     2221 ctggatctgg tccttgagga ccagcttgcg cttcttcagg cggcgcagct gcagctgatc
     2281 ctgcgcggga tcggcggaaa gccggttgat gatgtcgtcg aggtcgtggt gctccatctc
     2341 gagctcgtgc agatggagac gcacctgcac cttctcttcg tctgacagcg ggaactctgt
     2401 catgccaatg ccgcgctcct gacggaatcg agcggagtcc gggtgctgcc cttccgtgaa
     2461 agacttcatc gctcgcgctt ggcctttcgt ccatcctatc cctatacttg accaggggga
     2521 agggcccgcg cgaaggaaca aaaagcacag gcgcgcgggc tccagcatgc gccgtagcga
     2581 ccacgacgtt accgattcca tccgcaccac cgatccggcc gcggtcggcg cggaagtcgc
     2641 gcgcctattc aaagacctat acggcaaagt ttcggcggag gttgtggagc gcggcttccg
     2701 cgacgcgacc gccctctaca ggggcgagca tgcggactac tacccctgcg atactgaata
     2761 ccacgacatc cagcatgtgc tggacgtgac gctcgccatg gcgcggctga tggacggcta
     2821 cgagcgccac cgcaacgact cgccgcgcct ggacccggac gtgttcgagg tcggcatcgt
     2881 ggccgcgctg ttccacgact tcggctactt gcgccgcaag cgcgaccgcc tgcaccgcta
     2941 cggcgccgag tacaccctta cccacgtctc gcgcggcgcc gagtacctgc gcggctacgt
     3001 gggcgagctc ggcctggcgc ggcacgcgcg caccgccgcg gcgctcgtgc acttcaccgg
     3061 ctacgagcgc cgggtcgaga ccatccggct gtacgacgag ctgctgcgcc gcgtcggcca
     3121 catgctgggg acggccgaca tcattgccca gatgtcggac cgtt
//
LOCUS       DATLVW010000106         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_170955, whole genome shotgun sequence.
ACCESSION   DATLVW010000106 DATLVW010000000
VERSION     DATLVW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170955"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..595
                     /locus_tag="VNN25_03610"
     CDS             68..595
                     /locus_tag="VNN25_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH90643.1"
                     /translation="MRHRFAAVVLAAVALASPAFAAPKSTADTRTVSQLAAQAFPAPM
                     PKYLAGIIADAARTYDVDPNLIAAMTFRESAFNPNAVSSRGAQGLMQLMPRTAAALGV
                     RDAFDPRDNIFGGTRYVKTLLDRFHGNVELTLAAYNAGPELVAKVGPGATQEAIEYVA
                     AVKSFYQTAVATLQS"
     gene            629..1168
                     /locus_tag="VNN25_03615"
     CDS             629..1168
                     /locus_tag="VNN25_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HXH90644.1"
                     /translation="MSETQIDPRYPVGKFDRSTVPHTAEERRTFIDQIAAAPARMREA
                     VAGLSESQLDTPYRDGGWTVRQVVHHVPDSHMNAYTRVKLALTETEPTIRPYDEAAWA
                     KLNDVHDTPIAVSLTLLEALHDRWVRILRALTPDDFAKTLFHPEHGVMTLDTLVAMYA
                     WHSRHHVAHITSLRERSAW"
     gene            1252..2070
                     /gene="xdhC"
                     /locus_tag="VNN25_03620"
     CDS             1252..2070
                     /gene="xdhC"
                     /locus_tag="VNN25_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HXH90645.1"
                     /translation="MATFFDALNELMAAETPLVVVTVVDTAGSVPQDRGAKMIVTASG
                     LRFGTVGGGKVETKAIAEAQAMLRGEAGETTKFAQWNLAKDVGMTCGGIVKLYFESHN
                     VGRWCIVVFGAGHVANAVVNVLVHLDCHVTCIDPRAEWLAKIPDSPKLTKTLSSDMPS
                     LVKTLPDDAFVLLMTMGHTTDKPILLEILRTRSFPYLGVIGSDAKANILKRDIDEAGL
                     PDEAKRAFFCPIGLALGTNHPYEIAVSVAAQLLEVRDARLASRSLSEARDSSPP"
     gene            complement(2221..>2897)
                     /locus_tag="VNN25_03625"
     CDS             complement(2221..>2897)
                     /locus_tag="VNN25_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HXH90646.1"
                     /translation="AQGAVPGLASLTGARVVIQGFGNAGSIAAQLFRDTGATIIAVSD
                     STGGIFCADGIDPEMAIAHKQQSGSVVGLPGTTTVTNDDLLHIDCDVLIPAAFENQIR
                     ADNVDGVSTKLIAEAANGPTTPAADKILFARGIPVIPDILANSGGVTVSYFEWVQNIE
                     NEHWSEDQVNAHLRTKMEAATDAVLKMQIEIRDRSGADVDLRTAAFATAIKRVATLAL
                     ARGIWP"
BASE COUNT          522 a          951 c          863 g          561 t
ORIGIN      
        1 gagcttccga acgtgatccc gcatgacgcg ggacgttccg tgatccgaac cggaaaggag
       61 cgtccccatg cgtcatcgtt ttgctgccgt cgtgctcgcc gctgttgccc tcgcatcgcc
      121 tgcgtttgct gcaccgaaat cgaccgccga tacccgtacc gtcagccagc tggctgcgca
      181 ggcctttccc gcaccgatgc caaagtacct tgccggcatc atcgccgatg cggcgcgaac
      241 ctacgacgtc gatccgaacc tcatcgctgc gatgactttc cgtgagtcgg cgttcaatcc
      301 gaatgcagta tcgagtcggg gcgcccaggg actgatgcag ctcatgccac gcaccgccgc
      361 agccctcggc gtccgcgacg ccttcgatcc gcgcgacaac atcttcggcg gcacgcgcta
      421 tgtcaagacg ctccttgatc gctttcacgg taacgtcgag ttgacgctgg ccgcttacaa
      481 cgctggaccg gagctggtcg cgaaggtggg gccgggagcg acgcaggaag cgatcgagta
      541 cgtcgcggca gtgaagagtt tctaccagac ggctgtagcg acgctgcagt cctgaacttc
      601 gcttcgctca ggatagactt cgcaaaccat gagcgaaacc cagatcgatc ctcgctatcc
      661 cgtcggaaaa ttcgaccgct ccacggtgcc gcataccgct gaggagcgac gcacattcat
      721 cgatcaaatc gctgccgctc cggcgcgcat gcgcgaggca gtggcaggtc tgtcggagag
      781 tcagctcgac actccgtacc gcgacggcgg ctggacggtg cggcaagtcg ttcatcacgt
      841 acccgactcg cacatgaacg cctacacacg cgtgaagctg gcgctgacgg aaaccgaacc
      901 gacgatccgg ccgtacgacg aggccgcgtg ggcgaaactg aatgatgtgc acgacactcc
      961 gatcgctgtt tcgctcacgc ttcttgaagc gctgcacgat cgctgggtac gcattctccg
     1021 ggcgctgacc cctgatgatt tcgcgaaaac gctcttccat cccgagcacg gcgtgatgac
     1081 gctcgatacg cttgtcgcca tgtacgcgtg gcacagccgg catcacgtcg cacacatcac
     1141 ctctctgcgc gagcgcagcg cgtggtgacg tttcggatgc tgacggacgg cgatttcgtg
     1201 ggttgagatg agcctcgcgc tctgatcgct gctccgtcct acaatcgcgt tatggcaacc
     1261 ttcttcgacg cgctgaacga actgatggcg gccgagacgc cgctcgtcgt cgtcaccgtc
     1321 gtcgacacgg ccggcagcgt gccacaggat cgcggcgcaa agatgatcgt cacggcctcc
     1381 ggactccggt ttggaacggt tggcggtggc aaggtggaga ccaaagcgat cgccgaggcg
     1441 caagcgatgc tgcgcggaga agcgggggag acgacgaagt tcgcgcagtg gaatctcgcc
     1501 aaagacgtcg gcatgacctg cggcggcatc gtcaagctct acttcgaatc gcacaacgtc
     1561 ggccgctggt gcatcgtcgt ttttggcgcc ggccacgtgg caaacgcagt cgtcaacgtg
     1621 ctcgttcacc tcgactgcca cgtcacctgc atcgatccgc gcgcggagtg gctggcgaag
     1681 attcccgact caccgaaact gacgaagacg ctgtcgtccg acatgccctc gctggtgaag
     1741 acgctgcccg atgatgcgtt cgtcctcctg atgacgatgg ggcacacgac cgacaaaccg
     1801 atcctgctgg agatcctgcg cacccgctcc ttcccatacc tcggcgtgat cggcagcgac
     1861 gcgaaagcca acattttgaa gcgcgatatc gacgaggccg ggctgccgga cgaagcgaag
     1921 cgtgcgtttt tctgccctat cggcctcgcc ctcgggacga accaccctta cgagattgcg
     1981 gtgagcgtgg ccgcgcagct cctggaggtg cgggacgcgc gcctcgcttc acgctctctt
     2041 tcagaagctc gagattcatc gcctccgtga cgagggcgcg catcgtctcc gatgcgttct
     2101 ttttcgattt aatccaagca acctgcggcg gcgtgagcca gacggcaacg cgttccgtct
     2161 tcttctctcc ggccgtcttc ttctctccga cacccgaccc accactcccc acgccccgtt
     2221 tcacggccat atgcccctcg ccagtgccag cgtcgccact cgcttgattg cagtcgcgaa
     2281 agctgcggta cgcaggtcta cgtcagcgcc actgcgatcc cggatctcga tctgcatctt
     2341 caacacggcg tccgtggccg cttccatctt cgtgcgcagg tgcgcgttga cctgatcctc
     2401 gctccagtgc tcattctcga tgttctgaac ccactcaaag tagctgacgg tcacgccgcc
     2461 ggagttggcc aggatgtccg gaatcaccgg gatgcctcgg gcgaatagga tcttgtcggc
     2521 tgcaggcgtc gtcggtccgt tcgccgcctc ggcgatcagc ttcgtactga cgccgtccac
     2581 gttgtccgcg cgaatctgat tttcgaacgc cgcgggaatc agcacgtcgc agtcgatgtg
     2641 cagcagatca tcgttggtga ccgttgttgt tccgggcaga ccgacgaccg agccggattg
     2701 ctgcttatgt gcgatggcca tctccggatc gattccgtcc gcacagaaga tgccgcccgt
     2761 cgaatcgctg acggcgatga tcgtggcgcc ggtatcgcgg aagagttgtg ccgcgatcga
     2821 gccggcattg ccgaatccct ggatgacgac ccgcgcgccg gtgagtgatg cgagccccgg
     2881 cacagcgcct tgagcta
//
LOCUS       DATLVW010000107         5448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_210764, whole genome shotgun sequence.
ACCESSION   DATLVW010000107 DATLVW010000000
VERSION     DATLVW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210764"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /gene="dapA"
                     /locus_tag="VNN25_03630"
     CDS             <1..934
                     /gene="dapA"
                     /locus_tag="VNN25_03630"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXH90647.1"
                     /translation="DFGVTMTFRGLGTALITPFTDSGALDENALARFVDFQIDGGVDF
                     LVPCGTTGENPALTAAEHRRVVELVVHQANGRVPVLAGAGSNNTARAIELAEQAIDIG
                     AAGVLTITPYYNKPTPDGLRRHFGIQAEAIEKKKAGFPMIMYNVPGRTGVNMTAETTL
                     RMAAEIPNIIGVKEASANLEQIMMVIRDRANGFLVLSGDDAWTLALIGAGADGLVSVA
                     SNEIPRLMSELVTAALRGDFAAARAVHYRVLPLLTGNFMESNPIPVKTACKLLGILDS
                     DTVRSPLAPISKTNREKLEAILRECGLLESRPSS"
     gene            931..1758
                     /locus_tag="VNN25_03635"
     CDS             931..1758
                     /locus_tag="VNN25_03635"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXH90648.1"
                     /translation="MTSTIEERLATIGPKTDRMEAVRTIGLFLSELEAGSVRAAIRDE
                     DGVWHAQTWVKQGILLAFRVGVSAEFASGTLSFIDKDTIPTRRFRAQDGIRIVPGGSS
                     IRRGTFIAKGVVMMPPAYVNVGAYVDEDTMIDSHALVGSCAQIGKRVHLSAAAQIGGV
                     LEPVGNLPVIIEDDVVVGGNCGIYEGTVVRGRAVIGAGVVLTGSTPVYDTVRGQIYRR
                     SADRPLEIPYGAVVIPGSRMMKGDFAEKHALHVATPIIVKYRDEKTDASTALEDALR"
     gene            1892..3040
                     /locus_tag="VNN25_03640"
     CDS             1892..3040
                     /locus_tag="VNN25_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXH90649.1"
                     /translation="MRQPGNPATRQPPTAQRLANIELSLIRQINALATPLSVNLGIGE
                     PNVEPDETLRAFAVKAAATGSWHYTPNAGTLALRKLIAGERGFDPKSEVCVTAGTEEA
                     LFSIMQAFVDPGDEVLVPNPGFLAYATLARIAGATPVTYEIEPPDWRVDADALLARIT
                     PRTKLIVVNSPSNPLGSVIDCETLQRIANAGPLVVSDEVYREIWYETPPPSMLGMSDN
                     VIVVGGMSKSHAMTGLRLGWILAAETLMKPIVTAHQYIATCASAFSQSLAESILSDDA
                     WNRGWLERIRAQFRAQRESALFSIARELEVTIPAPAGAFYAFVPVPSCDTEMFARTLA
                     TDAAVLVIPGIAFGSMGEGFIRISYAASIDEIGTGVERIGRWLRGAAL"
     gene            3104..4006
                     /locus_tag="VNN25_03645"
     CDS             3104..4006
                     /locus_tag="VNN25_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH90650.1"
                     /translation="MALKARKAHEKLPVSPANDSVAASEISLYRNVLKIKEEHDLEEL
                     GISFWDAQTTIQWSYNADHYFHAASTMKLAVLIGVFREIDRGTLALDSLVHVRNRFTS
                     IVNQETFMLDLANDADPDVYGHLGKTLPVGELAYWMITKSSNLATNLLVDVVTIESIQ
                     QALDELEIDGVRVLRGVEDQAAFEAGLNNEVTANGLQKMLRVIADRKAFSPEASEEML
                     KIMLDQQYRSGIPAGLPKAARVAHKTGNISTVHHDAGIVFLEGREPYVLVILTQFRAE
                     TGRGSAVAEVSRDIYNTLAGLTYE"
     gene            3999..4691
                     /locus_tag="VNN25_03650"
     CDS             3999..4691
                     /locus_tag="VNN25_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90651.1"
                     /translation="MSDPQSIKVVDACDLDDELRFLLKPGGMVLDAQKRRHRLPRYFY
                     EIPSHEAAMSIRLTQHFGLNEFTLVDLKEEQRLQEFPRYIPCAVRVLAFYLEQFRKDA
                     GGSVHIAVNGGYRSPSHKLSEYATPHMWATAADIYRIGNTILKGKETIEKYNAIAEDS
                     SDDLHVLPYGHVTGLNADDHVHLDLGYITVIPREISEDRMEVPQENKPRFAFEDRRRV
                     ERRSQPVPEEGE"
     gene            4852..>5448
                     /locus_tag="VNN25_03655"
     CDS             4852..>5448
                     /locus_tag="VNN25_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90652.1"
                     /translation="MPVIGMESEFNVLLDEKEIDPRVFWGHPSAFIDRPLLPREKSSL
                     QLPTGGAVYFDRGVIEVVTPVIELAPHSTARTVRNLWEQIGFVRGQLTQWEKANDHTV
                     RLKAYSTHYNISYEIPKREQNAKRNEKALALLLAYILPVPVMLVASNRRSTGVGVRPR
                     GSRIEVTVDFTPDPGMMIAAATLIVGIVREVMTWPSYEL"
BASE COUNT         1081 a         1793 c         1608 g          966 t
ORIGIN      
        1 tgatttcgga gtaaccatga cctttcgcgg attaggcacc gccctcatca ctcctttcac
       61 tgacagcggc gcgctcgatg agaatgcgct cgcgcgcttc gtcgattttc agatcgatgg
      121 cggcgtcgat tttctggttc cctgcggtac caccggcgag aatcctgcgc tgaccgcggc
      181 ggaacatcgc cgcgttgtcg agctggtcgt gcaccaggcg aatggccgcg tgccagtgct
      241 cgcaggtgcg ggcagcaaca acaccgcacg agccatcgaa ttggccgaac aggccatcga
      301 catcggtgcc gccggcgtcc tgaccatcac gccttactac aacaagccga cgcccgatgg
      361 gctgcgccgt cacttcggga ttcaggccga ggccatcgag aagaagaagg ccggcttccc
      421 gatgatcatg tacaacgtgc cgggccgcac cggcgtcaac atgacagcgg aaacgacgct
      481 gcgtatggcc gccgagatcc ccaacatcat cggcgtgaag gaagcatccg cgaatctcga
      541 gcagatcatg atggtgatcc gcgatcgtgc gaacggattc ctggttctca gcggcgacga
      601 cgcctggacg ctggccctca tcggcgccgg cgcggacggt ctcgtttccg tcgcctcgaa
      661 tgagatcccg cgcttgatga gcgagcttgt gaccgcggcg ttgcgcggtg atttcgccgc
      721 cgctcgcgcc gttcactatc gcgttttgcc gctgctcacc ggcaacttca tggaatcgaa
      781 tcccatcccc gtcaagaccg cctgcaagct cctcggcatc ctcgacagcg acacggtccg
      841 ctcgccgctc gccccgatct cgaaaacgaa tcgtgagaag ctggaggcca ttctccggga
      901 atgcggactc ctggaatcga gaccctcgtc atgacgagca caatcgaaga gcggttggca
      961 acgatcggtc cgaaaacgga tcggatggaa gccgttcgca cgatcggact ctttctatcg
     1021 gaactggagg cgggctcagt ccgcgcagcc atccgcgacg aagacggcgt ctggcatgcg
     1081 cagacctggg tgaagcaggg aatcctgctc gcgtttcggg tgggtgtttc cgccgagttc
     1141 gcatccggca ctctttcgtt catcgacaag gacacgattc cgactcggcg cttccgcgca
     1201 caggacggca tccgcatcgt ccccggcggg tcgtcgatcc ggcgggggac attcatcgcc
     1261 aaaggcgtgg tcatgatgcc gcccgcgtac gtgaacgtcg gcgcctacgt cgacgaagac
     1321 acgatgatcg actcgcacgc gctcgtcggc tcgtgtgcgc agatcggcaa acgcgtgcac
     1381 ctctcggccg ccgcgcagat cggcggtgtg ctcgagccgg tcggcaatct tcctgttatc
     1441 atcgaagacg acgtcgtcgt cggcggcaac tgcggcatct acgaaggcac cgtcgttcgc
     1501 ggccgcgccg tcatcggcgc cggcgtggtc ctcacgggat cgacgccggt ctacgacacc
     1561 gtgcgcggcc agatctaccg ccgcagcgcc gatcgtccac tcgaaatccc ctacggcgca
     1621 gtcgtcatcc ccggctcgcg catgatgaag ggcgacttcg ccgagaaaca cgcgctccac
     1681 gtcgccacgc cgatcatcgt gaagtaccgc gacgagaaga cggacgcctc gaccgcgctc
     1741 gaagatgcgt tgcgatgaag gcaactttca aaatggggtc aggtctaaat ttgaaagtgc
     1801 cctcaaagct actgcgttac ctgttgccca aattgacact ttcaaattca gagctgaccc
     1861 cattttgaaa gttgccggag gctgaataat cgtgcggcaa cctggcaacc cggcaacgcg
     1921 gcaaccccct accgcccaac gcctcgccaa catcgagctc tccctcatcc gccagatcaa
     1981 cgcgctagcc acgcccctct ccgtcaacct cggcatcggc gagccgaacg tcgagccgga
     2041 cgaaacatta agagcgtttg cggtgaaagc agcggcgacc ggttcgtggc actacacgcc
     2101 gaatgccggg acgctcgcgc tgcgcaagct catcgccggc gagaggggct tcgatccgaa
     2161 atcagaggtc tgcgtcaccg ccggaaccga ggaagcgctc ttctcgatca tgcaggcctt
     2221 cgttgatccg ggtgatgaag tgctcgtccc caatcccggt ttcctcgcct atgcgacgct
     2281 ggcccgcatc gccggcgcga cgccggtgac ctacgagatc gagccgcccg attggcgcgt
     2341 cgatgctgat gcgttgctcg cgcgcatcac gccgaggacc aaactgatcg tcgtcaactc
     2401 gccgtcgaat ccgctcggca gcgtcatcga ttgcgagacc ttgcaacgca tcgccaacgc
     2461 cggaccgctc gtcgtcagcg atgaggttta tcgcgagatc tggtacgaaa cgccgccgcc
     2521 atcgatgctc ggcatgtccg acaacgtcat cgtcgtcgga gggatgtcga agagccacgc
     2581 gatgaccggc ctgcgtctcg gctggattct ggcagccgag acgctgatga aaccgatcgt
     2641 cacggcgcat caatacatcg ccacgtgcgc gtcagcgttc tcacaatcgc tagccgagtc
     2701 gattctttct gacgacgcct ggaaccgcgg atggctcgag cggatccgcg cgcagtttcg
     2761 cgcccaacga gagtccgcgc tcttctcgat cgcccgcgag ttggaggtca cgattccagc
     2821 gccggccggc gccttctacg cattcgtccc cgtgccgtcg tgcgacacgg agatgttcgc
     2881 gcgcacgctc gccaccgacg cggcggtact ggtcatcccc ggaatcgcct ttggatcgat
     2941 gggcgaagga ttcatccgca tctcgtatgc ggcatcgatc gacgaaatag gcaccggagt
     3001 cgagcgcatc ggccgctggc tgcgcggcgc agcgctttga ccggcgaacc gcgtgcaccg
     3061 aaacttgcaa cgaggtcgcc cgaggttcag taacattcgc caaatggcat tgaaggcccg
     3121 caaggcccac gagaagcttc cagtttcgcc ggccaacgac tccgtcgccg cgtccgaaat
     3181 ctcgctctac cgcaacgttc tcaagatcaa ggaagagcac gatctcgaag agctcgggat
     3241 ctcgttctgg gacgcgcaga ccacgattca gtggtcatat aacgccgacc actacttcca
     3301 cgccgcgtcg acgatgaagc tggccgtgct catcggcgtc ttccgcgaga tcgaccgcgg
     3361 aacgctcgct ctcgattcgc tggttcacgt ccgcaatcgc ttcacgagca tcgtcaacca
     3421 ggaaacgttc atgctcgatc tcgcgaacga tgccgatccg gacgtctacg gccacctcgg
     3481 caagacgctc ccggtcgggg agctcgccta ctggatgatc accaagtcga gcaacctcgc
     3541 caccaacctt ctcgtcgacg tcgtgaccat tgaaagcatc cagcaggctc tcgacgagct
     3601 ggagatcgac ggcgttcgcg tgttgcgcgg cgtcgaggat caggctgcgt tcgaggcggg
     3661 cctgaacaac gaagtgaccg cgaacggact gcagaaaatg ttgcgggtca tcgccgaccg
     3721 caaggcgttt tcgccggaag ccagcgagga gatgctgaag atcatgctcg atcagcaata
     3781 tcgaagcggc attccggccg gccttccgaa agcggcgcgc gtcgcgcaca aaaccggcaa
     3841 catatcgacc gtgcatcacg acgcaggaat cgtgtttctg gaagggcgtg aaccgtacgt
     3901 gctcgtcatc ctcacgcagt tccgcgccga aaccggccgc ggcagcgccg tcgcagaggt
     3961 ctcgcgcgac atctacaaca cgctcgcggg actgacctat gagtgatccg cagtcgatca
     4021 aagtcgtcga cgcctgcgat ctcgacgatg agctgcggtt tctgctcaag cccggcggga
     4081 tggtgctcga cgcacagaaa cgccgtcatc ggctgccgcg ctatttctac gagatcccga
     4141 gccacgaggc ggccatgagc atccgcctga cgcagcattt cggccttaac gagttcactc
     4201 tcgtcgacct caaggaagag cagcggctgc aggagttccc ccgctacatc ccctgcgccg
     4261 tccgcgtcct ggcgttctat ctcgaacagt tccgaaagga cgctggcggc tcggttcaca
     4321 tcgcggtcaa tggcggctac cgttcgccgt cccacaagct gtcggagtac gcgacgccgc
     4381 atatgtgggc caccgcggcc gatatctacc gcatcggaaa cacgatcctg aaggggaaag
     4441 agacgatcga gaagtacaac gccatcgctg aggattcgag tgatgatctt cacgtgctgc
     4501 cgtacggcca cgtcaccggt ctcaatgccg acgatcacgt ccatctcgac ctcgggtaca
     4561 tcacagtgat cccccgcgag atcagtgaag accggatgga agtgccgcag gaaaacaagc
     4621 cccgtttcgc cttcgaggac cgccgccgtg tcgagcgccg gtcccagcca gtgcccgaag
     4681 aaggcgaata actacgctgt gcccagcacc cggcctgccg tttgcttaga ggacgtatgc
     4741 agtagctcca tacatcccaa cgcgacggcc cggaagattt gcatgcctgg aacgccagat
     4801 ttgaaacaac agagaccagt cgggaagctc ctccagcgga ctcgactgga gatgccggtc
     4861 ataggcatgg agtcggaatt caacgtcctt ctcgacgaga aggagatcga tccgcgcgtg
     4921 ttctggggcc atcccagcgc gttcatcgac cggcctcttc ttccgcgcga aaagtcgtcc
     4981 ctgcagcttc cgaccggagg cgcggtctac ttcgatcgcg gcgtcatcga agtcgtcacg
     5041 ccggtcatcg agctggcccc gcactccacc gcccgcaccg tgcgcaacct gtgggaacag
     5101 atcggcttcg tccgcggcca gctcacgcag tgggaaaagg cgaatgatca caccgtccgg
     5161 ctgaaggcct acagcaccca ctacaacatc tcctacgaga tcccgaaacg agagcagaac
     5221 gcgaagcgca acgagaaagc gctggcgcta ttactcgcct acatcctgcc cgtgccggtg
     5281 atgctcgtgg cgtcgaaccg ccggtcgacc ggagtcggcg tccggccgcg tggcagcagg
     5341 atcgaggtca cggtcgattt cacgcccgac cccggcatga tgatcgccgc ggccacgctc
     5401 atcgtcggca tcgtccggga agtgatgacg tggccctcgt acgagctg
//
LOCUS       DATLVW010000108         4732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_171133, whole genome shotgun sequence.
ACCESSION   DATLVW010000108 DATLVW010000000
VERSION     DATLVW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171133"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(866..1636)
                     /locus_tag="VNN25_03660"
     CDS             complement(866..1636)
                     /locus_tag="VNN25_03660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXH90653.1"
                     /translation="MPRFERYVAIGDSTVEGLDDPDGRGGYRGWADRLAARLAAEQGS
                     VLHANLAIRGRTTHQIRQEQLDPAVAMRPDLVAVVSGTNDLLRRRFDPVALRDDVETM
                     QGTLTAIGATVITFTLPDLGDVMPLARIVRGRLVQMNQIIRDAASKTGAIVCDFAAYP
                     VASDPRLWSDDRLHANTAGHARIANALADHLGLAGADRSWSEPLPDAPPPRWTDQLRA
                     EAAWTRRHLLPWIWRHAQGRSSGDGITPKQPQLVLIEG"
     gene            complement(1651..3816)
                     /locus_tag="VNN25_03665"
     CDS             complement(1651..3816)
                     /locus_tag="VNN25_03665"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXH90654.1"
                     /translation="MIRFEDILDKVESYKPDFDEELLDKAFVFSAREHRGQVRSSGEP
                     YLVHPLNVAYILAEMRLDETSIAVGLLHDVLEDTLTTKETLQELFGDDVAELVDGVTK
                     ISRYAYVSKEEQQAETFRKMLLAMVSDLRVVLVKLADRLHNMRTLEFLPEERRLAIAT
                     ETMEIYAPIANRLGMGRIKSELEDLAFRHMHPRECEELQRSVNDKLAVSGDIVDRIKS
                     TLTAKLAENDINGDVFGRVKSMYSIWTKLRRQEIDVGQVYDYLAFRIVTANVKDTYAC
                     LGIIHQMWRPVPGRIKDYIAMPKPNFYQSLHTTVVADKGQPFEVQIRTREMDLIAEQG
                     IAAHWKYKEGRIGAQADDSNFLWLRQLVEWQHEITDPRVFMQSLKIDLYPDEVYTFTP
                     RGDVFAFPRGATPLDFAYRIHTDVGHHCVGARINGKLVPLRTTIKNGDIIEVITAPTQ
                     SPSREWINMVVTSRARHKIRHWLNTEQKHRSIDLGRKLIEKEAKRYRVQWRKLVAENA
                     LDAVLAEYSLPRLDDLYADVGYGKVSPRAVVERFVKDEQPPVDEGVIQRAVRKIFPFT
                     GTSTAIKVKGYDDLMTYLAKCCNPLPGERIIGYISRGKGVGVHSANCPNVRNLMFNAD
                     RQIAVEWADQRSAQFQIELEIAMEDRQGILAKVVSTVSNMKTNIRQMDSRSTDGKATA
                     ELTLEIADLKHLERIIRAIGDIEGVVAVERKFNVRHATA"
     gene            4008..4370
                     /locus_tag="VNN25_03670"
     CDS             4008..4370
                     /locus_tag="VNN25_03670"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HXH90655.1"
                     /translation="MSKRSSQLVAFVLVVAFAALPVFAAPVPSKTAADQSMDQRAADL
                     ALVRDVTANEQVAKVLADRGFTKDQVDQRLAQLSPQDLHQLAQNVNQMQAAGLTRQEW
                     IWIGIGALAALILVIAVG"
     gene            4465..>4732
                     /locus_tag="VNN25_03675"
     CDS             4465..>4732
                     /locus_tag="VNN25_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90656.1"
                     /translation="MLNVECVSRSRFTLHASLFTLLFTLLLTSCITITPKTQHASPSA
                     SVISNVPMQHWGIESCGAGSLSTVLQHYGDATTMRTWDDTLPKTR"
BASE COUNT          829 a         1429 c         1490 g          984 t
ORIGIN      
        1 cgcgatgcgg ttcgcggtct gtatgacgtg aaacggcttc aacccgagag cggccatgac
       61 tgcggccaac ggagttgtgt gctctttccc atccactcgc gcgattcctc cccggctccg
      121 tagtgggagt gtcgtctggc atgagattcc ctacctccgg catgaaacga ccactatagg
      181 gggcgtctga gccattctga ctgcgcgagt gcgcaagaag tgacacatat gttccacatc
      241 gctttctcga ttgccgtctt tgtcgcgatc cttcaggtgc cgcatgaggc tgcgctgggg
      301 cgtgccgtcc cggatacagc ccgctctcgg gttttcgtcc cgaggagacg cccttgatga
      361 cgtcaccacg gctgcgccgg gtgaaacttg cctctcgacg gtgaccacaa ttatcacacc
      421 taaccccctg gacgccttcc ttcttgggag gcgctgatcg cctgatgacg acgcagaaaa
      481 acgcttactc gggagcagaa tctccttccc aaacagggct ttcaggcctg atgaggaaag
      541 aaaaacgctc ttcaagacaa gtatagtgtt ctttaagaca acgatagtcc tctttaagac
      601 aaccaggtcc tctttaagac aaccatagtc ctctttaaga caaccattgt cctctttaag
      661 acaaccatag tcctcttaaa gacaaccatt gtcctcttaa agacaaccat agtcctctta
      721 aagacaagca tagtgttctt gaagaacgat ttttcctttc ggcttgtcta aaaatcagtt
      781 ggttggaaag cgcagtcggc aacggcgcac gactttgtgt catcagtgcc tctgccgtcc
      841 gttgtgcccc ggccaccgcg aggctctacc cttcgatcaa gacgagctga ggctgcttcg
      901 gcgtgatgcc gtcgccagac gatcggcctt gcgcgtggcg ccagatccat ggcaacagat
      961 gccggcgcgt ccaggcggcc tcggcgcgca actggtccgt ccatcgcggc ggcggcgcgt
     1021 ccggcagcgg ctcggaccat gatcgatcgg cgcctgccag gccgaggtgg tcggcgaggg
     1081 cgttggcgat gcgggcatgg cccgcggtat tggcgtggag ccggtcgtcc gaccagaggc
     1141 ggggatccga tgccaccgga tacgccgcaa agtcgcagac gatcgcgccg gtcttcgaag
     1201 cggcgtcgcg gatgatctga ttcatctgca ccaagcgtcc gcgaacgatg cgggcgagcg
     1261 gcatcacatc gccaaggtcg ggaagggtga aggtgatcac ggtggcgccg atagcggtca
     1321 gcgtgccctg catcgtttcg acgtcgtcgc ggagggccac aggatcgaag cgccgccgca
     1381 ggagatcgtt ggttccggag acgactgcga ccagatccgg acgcatcgca acggcggggt
     1441 cgagctgctc ctgccggatc tgatgggtcg tccttccgcg aatggcgaga tttgcgtgga
     1501 gcacgctacc ctgctccgcg gcgagtctgg cggcaagccg gtccgcccaa cctcgataac
     1561 cgccccgccc atcgggatcg tcgagccctt cgaccgtgct gtctccgatg gcaacgtagc
     1621 gttcgaatcg gggcatgccg gccggcgcgg tcacgccgtg gcgtgccgca cattgaactt
     1681 gcgctcgacg gcaaccaccc cttcgatgtc cccgatggcc cggatgatgc gctcgagatg
     1741 cttgaggtcg gcgatctcca gcgtcagctc ggcggtggcc ttgccgtcgg tactgcggct
     1801 atccatctgg cggatgttcg tcttcatgtt ggagacggta gagacgacct tggccaggat
     1861 gccctgccgg tcttccatgg cgatctccag ctcgatctgg aactgcgccg accgttgatc
     1921 cgcccactcg acggcgatct gacggtcggc gttgaacatg aggtttcgca cgttcggaca
     1981 gttggcgctg tgcaccccga cgcctttgcc gcgggagatg tagccgatga tccgctcgcc
     2041 cggcagggga ttgcagcact tggcgaggta ggtcatgagg tcgtcatagc ctttgacctt
     2101 gatggcggtg gaggtgccgg tgaagggaaa gatcttccgc acggcacgct ggatgacgcc
     2161 ttcatcgacg ggcggctgct cgtccttgac gaagcgctcg acgacggcac ggggcgagac
     2221 tttgccgtag ccgacgtcgg cgtagaggtc gtcgagccgt ggcaggctgt actcggcgag
     2281 cacggcgtcg agcgcgttct ccgcgacgag cttccgccac tgcacgcggt accgtttggc
     2341 ctctttctcg atcagcttcc ggcccaaatc gatggagcgg tgtttctgtt cggtgttgag
     2401 ccagtgccgg atcttgtgcc gggcccgcga ggtgaccacc atgttgatcc actcgcggga
     2461 aggcgactgc gtcggcgcgg tgatgacttc gatgatgtcg ccgttcttga tggtcgtccg
     2521 cagcggcacg agcttgccgt tgatgcgcgc accgacgcag tgatggccga cgtcggtgtg
     2581 aatgcgatat gcgaagtcga gcggcgtggc tccccgcgga aaggcgaaga catcgccccg
     2641 cggcgtgaac gtgtagacct cgtcgggata gagatcgatc ttcagcgact gcatgaagac
     2701 gcgcggatcg gtaatctcgt gctgccattc gacgagctgc cgcagccaga ggaagttgga
     2761 atcgtcggcc tgcgctccga tgcgcccctc tttgtatttc caatgcgcgg cgatgccctg
     2821 ctcggcgatg aggtccatct cccgcgtccg gatctgcact tcgaacggct gccctttgtc
     2881 tgcaacgacc gtcgtgtgca acgactggta gaagttgggc ttgggcatgg caatgtagtc
     2941 tttgattcgt cccgggaccg gacgccacat ctgatggatg atgccgaggc aggcgtaggt
     3001 gtccttgacg ttggcggtga cgatgcggaa cgccaggtag tcgtagacct ggccgacgtc
     3061 gatctcctgc cgccgaagtt tcgtccagat ggagtacatc gactttaccc ggccgaagac
     3121 gtcgccgttg atgtcgtttt cggccagctt ggccgtaagg gtgctcttga tgcggtcgac
     3181 gatgtccccg gacacggcca gcttgtcgtt gacgctgcgc tgcaattctt cgcattcgcg
     3241 cggatgcatg tgccggaagg cgagatcttc gagctcgctc ttgatgcggc ccataccgag
     3301 gcggttggcg atgggcgcat agatctccat cgtctccgtg gcaatggcca gccttcgctc
     3361 ctcgggcaag aactcgagcg tgcgcatgtt gtgaaggcgg tcggccagct tgacgaggac
     3421 gacgcgcagg tcgctcacca tggccaggag catcttgcgg aacgtctcgg cctgctgctc
     3481 ttccttcgac acgtaggcgt accgggagat cttcgtgacg ccgtccacca gttcggccac
     3541 gtcgtcgcca aagagctcct gcagcgtctc cttcgtcgtc agggtgtctt cgagcacgtc
     3601 gtgcaggaga ccgacggcga tggaggtctc gtcgaggcgc atctcggcga ggatgtaggc
     3661 gacgttgagg ggatggacga ggtagggctc gccggagctg cgcacctgcc cgcgatgctc
     3721 gcgggccgag aagacgaaag ccttgtcgag cagctcctca tcgaagtcgg gcttgtagct
     3781 ttcgactttg tcgaggatgt cttcgaagcg gatcacggca gttggtaaca ggtttggaat
     3841 cgtagctgat tctcccccgg cgtgcggcgc gcagatccgg gcattcttat tgctcccgtc
     3901 cgctagttgg atgccgtcgc cacgggtcgt gggccctgga tgccgggatt aactgaaacg
     3961 gtgaaggacg gtcgaaaccg gcctcacaca acatggaggt aattgtcgtg tccaagcgtt
     4021 cgtctcaact cgtagcattc gttctcgtcg tcgcttttgc agccctgccg gtgttcgctg
     4081 cacccgtccc gtcgaagacc gccgcggatc agagcatgga ccagcgggcc gccgacctcg
     4141 ccctcgtccg tgacgtcacg gcgaacgagc aggtggccaa ggtactggcg gaccgcggct
     4201 tcacgaagga tcaggtcgat cagcgcctgg cccagctctc gccgcaggac ctgcaccagc
     4261 ttgcgcagaa cgtgaaccag atgcaggccg ccggcctgac gcgccaggaa tggatctgga
     4321 tcggcatcgg cgcgctggct gcgttgatcc tcgtcatcgc cgtcggttga tgaaactgtt
     4381 tcgccgtagc ttccgggcga gcgggctggt tgggccgctc gctcggtgtt tttgccgggt
     4441 ttgtagagaa tggggagagc cgaaatgttg aatgttgaat gcgtgtcccg ttcacgcttc
     4501 actcttcacg cttcactctt cactctcctc ttcacactac tcctcacatc ctgcatcacg
     4561 atcaccccca aaacgcaaca cgcctccccg tccgcctcgg tcatctccaa tgttccgatg
     4621 cagcattggg gcatcgagtc gtgcggcgcg ggatcgctgt cgaccgtact tcagcattat
     4681 ggcgacgcca cgacgatgcg aacgtgggac gacacgctgc cgaagacacg cg
//
LOCUS       DATLVW010000109        12250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_337329, whole genome shotgun sequence.
ACCESSION   DATLVW010000109 DATLVW010000000
VERSION     DATLVW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337329"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNN25_03680"
     CDS             <1..609
                     /locus_tag="VNN25_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH90657.1"
                     /translation="DLYQVHNPTLDELRREEIQDAMERLQDAGKIRYWGVSVSSPEEG
                     IEIIKRGWGHALQVLYNILNQAPAQELFPLAKEKGYGIIARVPLASGLLTGKFRNDTT
                     FTSDDLRQNFLTPRRLQEALDRVDEVKSIIGGTARSLIEGALRFVLANDAVATAIPGA
                     KNVRQVETNVTAGDAWLPEDVVEKLRTRLGDYNFYQRHNVRI"
     gene            685..3525
                     /gene="uvrA"
                     /locus_tag="VNN25_03685"
     CDS             685..3525
                     /gene="uvrA"
                     /locus_tag="VNN25_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXH90658.1"
                     /translation="MPIRTISIRGARTHNLKNVSVDIPQRQLTVLTGPSGSGKSSLAF
                     NTLYAEGQRRFVESMSTYVRQFLERIDRPDVDEIDGILPAIAIEQKNSVKNARSTVAT
                     ATELADHIRLFMTYAGETFCPDCQVKVRRETPESITSAIIDSLAGKRVVVLAPIFFGA
                     ENRGEVLMQLVKAGYFRAWIGGEVHDLNETHTTGFTSLELVINRLRVDAERSGQITEA
                     IEQAFEVSKGNVNILEDAGDGNWISHRFTSRFSCNKCGTEFVEPTPHMFSFNSPLGAC
                     TNCQGYGRIIGIDMEKVIPNRALRLDEMPIAPWNSPGYEDCYDDLKKAAAKYKFRMDV
                     PIKDLSPEEWTLLYEGRSKWYGIKGFFEWLETKKYKIHVRVKLAKYRSYEPCPQCHGS
                     RLKISASYVTFRDKTVNDLFAMNVRDARRFWEYLPFAKSEESKFGHLRREIVNRLTYL
                     DEVGLSYLTLDRQTRTLSGGESQRINLAAALGSSLTETMYVIDEPTVGLHARDSERLL
                     AVLKRLKNAGNTVIVVEHDPTIIAGADQTIELGPGAGEYGGSVLYVGPPRPAVIPTES
                     DTTPATRNRAADAGFGAITLRGAREHNLQNIDVTIPLGKFVAVTGVSGSGKSTLIRNC
                     LYNRYQRDVRGATGLDVGRVAALEGTDKIYDMQFIDQSPIGRSTRSNPATYVKAWDEV
                     RKILSATTAAKLNNVTAGMFSFNTIGGRCDECEGAGTVTIDMQFLADVEVICDKCGGK
                     RFNEQVMKVTYKNKNVNDILEMTVDEAMKFFVDKRALLKKLAALRSVGLGYLRLGQST
                     ASLSGGEAQRLKLASFLSESTKSDNGHLFLFDEPTTGLHYTDVAQLIKTFRNLIDRGN
                     SVLVIEHNIQLIEAADHIIDLGPEGGDGGGELLAVGTPEEIAACERSITGRYLAKSSL
                     LRDLEVSMRQIDAGQVLSNKDAKPELLRRFTP"
     gene            complement(3570..4064)
                     /locus_tag="VNN25_03690"
     CDS             complement(3570..4064)
                     /locus_tag="VNN25_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH90659.1"
                     /translation="MLPEERLRAAIASASPHLVAISDADAAKPTAPGKWSPKQIIGHL
                     IDSASNNHQRFVRANFTDDLIFPGYDQEKWVSLGRYADAPWESLLSLWREFNLQIARV
                     MEATPADVRDVPRARHNLHQLAWRAVPENESATLEYFMSDYVDHLKHHLGQIWMMDSE
                     TTHG"
     gene            complement(4064..4411)
                     /locus_tag="VNN25_03695"
     CDS             complement(4064..4411)
                     /locus_tag="VNN25_03695"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HXH90660.1"
                     /translation="MPDPTVEQHFSGRADHVRRIYDTIVNASSALGPVEQDPKKTSIH
                     LNRRVAFAGVQTRKDALILTLKSDRDIKHRRVHKTEQTSANRWHFEVRLAAPEEVDDQ
                     VSKWIQASYELAQ"
     gene            4562..6940
                     /locus_tag="VNN25_03700"
     CDS             4562..6940
                     /locus_tag="VNN25_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXH90661.1"
                     /translation="MSAVEKDYIAPVPAPAPFEPYIPAATNMRELTPLPIIVGTILGM
                     IFGAASLYLVLKVGLTVSASIPVAVLSITFFRILSKLGGRDATILENNIVQTAGSAGE
                     SIAFGVGVTMPAILILGFDLEFTRVMLVAVLGGLLGILMMIPLRRALIVAQHGTLKYP
                     EGTACAEVLKAGASEESIAAASDSAKAEMAKLGGQATSAKTIFAGFGIGFIYKAMNVA
                     FRWWKDTPERVFGAPFAAGSVSAEISPELLGVGYIIGPRIASIMCAGGVLAYLVLIPA
                     IKFFGAGLAAPLAPEATRLIKDMSPNAIRSAYILYIGAGAVAAGGIISMLRSLPTIWH
                     GLKEGLKDMRGGSAAREGMVRTDRDLSMKLVLIGSFALVAIIALASPLYHGGTGVMAR
                     VMAAILIVVLGFLFVTVSSRLTGEIGSSSNPISGMTVATLLITCLVFLVIGWTGSTYY
                     VTALSIGAIVCIASSNGGTTSQDLKTGYLVGSTPKYQQIAILIGALASALVLGPLLLM
                     LNNNGSVVLPRISSEPSTGEEGAPLKQVENWAPGLHAEPSILATLSHKSYEGKDYLVW
                     HYTPAGGASAQELLVDPSGTPQYLVDPAINGTHKFLANGKLAPKKFDAPKATLMSYII
                     KGILNRQLPWGLVLLGVMISLVLEMSGIPSLAFAVGVYLPLSSSSPIFIGGMIRWLSD
                     LYVRKKYRDANLTEEQLVAEGDKSPGVLMASGYIAGGAIAGILIAFIAGLQDKVAWVT
                     NFNASLDRFADKNPFVSGAHSDLLSCIPFVFLILLLYLVGREILMAPKTRST"
     gene            7023..10667
                     /locus_tag="VNN25_03705"
     CDS             7023..10667
                     /locus_tag="VNN25_03705"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXH90662.1"
                     /translation="MESLGCVVFLVAVFALITFVVAIRASGRAGEATREVAELKRRLE
                     RMSQRVSHAESAAAGFADEDLRHAPAAGGVGKVPEPMPAWTGAPVRDEIALLPEEMQP
                     PAPTLPEPAPIVVPPVAAFTAEAPEPVAVLDEAISEPEPPREIPPPPPPTPHPPRPAP
                     PPKRPFDWESLIGVKLFSWIAGIALVLAALFFLSYSVEHGWLSPPVRASLGLLTGVAL
                     LLVCELRVARNYAFTANAMDGAGIAILYATLFAIHALWHLLPAAAVFALMLLVTAIAV
                     MLSIRRDSVFIALLGLVGGFATPALLSTGENKPIALFSYLLLLNVGLMWVAIRRRWPL
                     LTALSVIFTVIYEWGWIGKFLTGSQLPLAVAIFTVFAAAAASSLWMRRRADAKQGSFD
                     LVAISSAGLPLLFAIYVAAVANYGVQYNVLFTFLLLITSGLSVIAIVRGPAWLHLLGG
                     GTIALVLLVWRVSSYTPQAWPAILAWVAGFVVVQLVLSHFAPNPRAVLVPLLLLILPT
                     LAATPIPAAAPLLLFGALFVLVALIAAYAFMHHAGEAYAVGAFLAIVTEGVWSAQSIT
                     PVNLTTALLIYAAFALLFIGIPVIGRRLGREIVSPRTMLGLLLGSIAILFFLATGSVA
                     TYSLLALAILLVIINAGAIAEARKKEQAVLAAAAIVLSWTVITTWCASAMTAANLVAA
                     LSVIGGFALLAVGGGVWLSRGDDEDAGTTYLGLIGHLFLIVIATQPALAFPPWPLFAV
                     LFVLDLAIGVAAIYLRRASLMAAAMAGSQIVLMAWAANTKAAPWPVVAIGAAIAVCAM
                     ALVWFRIDRRFAIAALIALFLGDVVLIIAGFNAGTPLFASLLAAHLAIGIAILAVTWL
                     TEWHEPAVVAVALLAVATALARTSTPGTRLTFAGAIYAIFIIYPLLLGGRAKRSLYPY
                     LAAVLASVPFFIVARDAVRDAGYNYAIGVLPLFQAALMLLLLWRLLRAEPATERLLSR
                     LALVAGAALAFITVAIPLQLARQWITIAWALEAAALIWLFRRIAHRGLLIWSGALFAA
                     VFVRLLFGDDVFVHTASRLPIVNWYLYTYVVSAAAFFAAARLLPDSEAKQYPFARPVL
                     SSGGTILLFFLVNIEIADFYSTGSVLTFNFFSSSLAQDLSYTIAWAVFAVAMLIAGLF
                     LHSRATRIAAIILLLVTVLKCFLHDLARLGGLYRVGSLLGLTISLLLVGVLLQKFVIR
                     KAAPPTGEAA"
     gene            10664..11488
                     /locus_tag="VNN25_03710"
     CDS             10664..11488
                     /locus_tag="VNN25_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90663.1"
                     /translation="MIAAAFLLAALTLPEVPSGAPVDRGEFLYARPLGTIPAGVVSLT
                     LDAHVLLRSHALQDVRIVDAKGNQVPYLVIPLTAPTIVSLAIPPAAREGKQSVYRLTL
                     PYESLPNGAELELTTSASVFERDVVLRRALDESRGREAEVIDRLSWKSMPSMRASLTG
                     GRAIEVVVDEGDNAPLPIVSAKLRLPGFALRFVSPGTPLTLLYGNSSISAPRYDLALI
                     SSQLVGQPARAIALIGEPSTEPPAKHRESRLFGIAIGVAAAVLLIVLGRLVSAQRS"
     gene            complement(11469..11825)
                     /locus_tag="VNN25_03715"
     CDS             complement(11469..11825)
                     /locus_tag="VNN25_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90664.1"
                     /translation="MEKPVRRTLLPGVSDAAVPPPVVIDSRVVVARARRRAIVRDTVD
                     FLLLVCVDGLFLRWPGAHVPFLDRGDSLTVVVGLNVMLVAYLWLARSIPRWTARRVAT
                     TWSLAERTRFFRTAAR"
BASE COUNT         2094 a         4026 c         3701 g         2429 t
ORIGIN      
        1 gatctgtatc aagttcacaa tcccacgctc gacgagctgc gccgcgaaga gattcaggac
       61 gcgatggagc ggctgcagga tgcgggcaag atccgttact ggggagtgtc ggtttcgagt
      121 cccgaggagg gaatagagat cattaagcgt ggatggggcc acgcgctgca ggttctctac
      181 aacatcctga atcaagcgcc ggcgcaagag ctgtttccgc tggcaaaaga gaaaggctac
      241 ggcatcatcg cccgcgttcc gctggcctcc ggccttctca cgggaaagtt tcgcaatgac
      301 accaccttca cctcggacga tctccgccag aatttcctga cgccccgccg cctgcaggaa
      361 gcgctcgacc gcgtcgacga ggtgaaatct atcatcggcg gcacagcccg cagcctgatc
      421 gaaggcgcgc tccgcttcgt tctcgccaac gacgccgtcg ccaccgccat ccctggtgcg
      481 aagaacgtcc gtcaggtgga gaccaatgtc accgccggcg acgcctggct ccccgaggat
      541 gtcgtcgaga aactccgcac gcggcttggg gactacaact tctaccagcg gcacaatgtg
      601 cggatctgaa gtgtcgaagc ttaaccaata gatagaacca gaaacgcgga acagcgccaa
      661 cccgcaaaac gttctcaact cgacatgccc attcgcacga tctccattcg cggggcgcgg
      721 acacacaatc tcaagaacgt ttcggtcgat attccgcagc gccagctcac ggttctgacc
      781 ggaccgtccg ggtcagggaa atcgtcgctg gcgttcaata ccctgtatgc cgaggggcag
      841 cggcggttcg tcgaatcgat gtcgacgtac gtgcggcagt tcctggagcg catcgaccgg
      901 cccgacgtcg atgagatcga cggcatactc cccgcgatcg ccatcgagca gaaaaactca
      961 gtcaagaacg cgcgatcgac ggtggcaacc gcgaccgagc ttgcggacca catccgcctt
     1021 ttcatgacct atgccggcga gacgttttgc cccgactgcc aggtgaaggt gcgtcgcgaa
     1081 acgccggagt cgatcaccag cgcgatcatc gactcgctcg cggggaagcg cgtggtcgtg
     1141 ctcgccccga tcttcttcgg cgccgagaac cgcggcgagg tgctgatgca gctcgtcaag
     1201 gccggctact tccgcgcctg gatcggcggc gaggtgcatg acctcaacga gacacacacg
     1261 accggtttca cgtcgctcga gctggtcatc aaccggctgc gcgttgatgc cgagcggagc
     1321 ggccagatca ccgaggccat cgagcaggcg ttcgaggtct cgaaaggcaa cgtcaacatt
     1381 ctcgaagacg ccggcgacgg aaactggatc agccaccgct ttacgtcgcg cttttcctgc
     1441 aacaagtgcg gtaccgagtt cgtcgagccg acgccgcaca tgttctcctt caactcgccc
     1501 ctcggcgcct gcacgaactg ccagggctac ggccggatca tcggcatcga catggagaag
     1561 gtgattccga atcgtgcttt gcgcctcgac gagatgccga tcgcaccgtg gaactctccc
     1621 ggctacgaag attgctacga cgatctgaag aaagccgccg ccaagtacaa gttccgcatg
     1681 gacgttccga tcaaagatct gtcccccgag gagtggacgc tgctgtacga agggcggagc
     1741 aagtggtatg gcatcaaagg cttctttgag tggctggaga cgaagaagta caagatccac
     1801 gtccgcgtga agctggccaa ataccgcagc tacgagccct gcccgcagtg tcacggctcc
     1861 cgcctcaaga tctcggcatc gtacgtgacg ttccgcgaca agaccgtcaa cgacctgttc
     1921 gcgatgaacg ttcgcgacgc gcgacgtttc tgggagtacc tgccgttcgc gaagtcggag
     1981 gagtcaaagt ttggccacct ccggcgcgag atcgtgaatc gcctgaccta tctcgatgag
     2041 gtcggactct cgtatctcac gctcgaccgc cagacgcgca cgctctccgg cggcgaatcg
     2101 cagcgcatca atctcgcagc agcgctcggc agctcgctga ccgagactat gtacgtgatc
     2161 gacgaaccaa cagtcggcct ccacgcacga gacagcgaac ggctgctggc cgttctcaag
     2221 cgtctcaaga acgccggcaa taccgtgatc gtcgtcgaac acgatccgac gatcatcgcg
     2281 ggcgcggatc agacgatcga gttgggaccg ggagcaggcg agtacggcgg ttcggtgttg
     2341 tacgtcggac cgccgcggcc agccgtcatt cctactgagt cggataccac gcctgcgacg
     2401 aggaaccgcg ctgccgacgc cggcttcggc gccatcacgc tccgcggcgc ccgtgagcac
     2461 aacctccaga acatcgacgt gaccattccg ctcggaaagt tcgtcgcggt caccggcgtc
     2521 tccggctccg gcaagtcaac gctgatccgc aactgcctct acaaccgcta ccagcgcgac
     2581 gtccgcggcg cgacgggact cgacgttggc cgcgtggccg cgctcgaagg caccgacaag
     2641 atctacgaca tgcagttcat cgatcagtcg ccgattggcc gctcgacgcg ctcgaatccg
     2701 gccacgtacg tgaaagcctg ggacgaggtc cgcaagatcc tctccgcgac gaccgcggcg
     2761 aagttgaaca acgtcaccgc tggcatgttc tcgttcaata ccatcggtgg acgctgcgac
     2821 gagtgcgaag gcgccggcac agtgacgatc gacatgcagt tcctcgctga cgtcgaggtc
     2881 atctgcgaca agtgcggcgg caagcgcttc aacgagcagg tcatgaaagt cacgtacaag
     2941 aacaagaacg tgaacgacat cctggagatg accgtcgacg aagcgatgaa gttcttcgtc
     3001 gacaaacgcg ccctactcaa aaagctggcc gccctccgct ccgtcggcct cggctacctc
     3061 cgcctcggcc agtccaccgc ctccctctcc ggcggcgaag cgcaacgcct caagctcgcc
     3121 tcgttcctct ccgagagcac caagagcgac aacggtcacc tcttcctctt cgacgagccc
     3181 acgaccggcc tccactacac cgacgtcgcc cagctcatca agacgttccg caatctcatc
     3241 gaccgcggca actcagtcct cgtgatcgaa cacaacatcc aactcatcga agccgccgac
     3301 cacatcatcg acctcggccc ggaaggtggg gatggaggtg gcgaactgct cgccgttggg
     3361 acgccggagg agatcgcggc gtgtgagagg agtatcaccg ggcgttacct tgcgaagtcg
     3421 tccctcctcc gtgatttgga ggtctccatg aggcagatcg acgccgggca agtcttgtcc
     3481 aataaagacg cgaagccgga actgcttcgt cggtttactc cctagctccc cgcgttttcc
     3541 gtcagcacac tgtccgcttt gccgtttggt tagccatgag tggtttctga gtccatcatc
     3601 cagatctgcc ccaaatggtg cttcaaatga tccacgtagt cgctcataaa gtactcgagc
     3661 gtcgccgact cattttccgg aaccgcgcgc caagccaatt gatggagatt gtgtctagca
     3721 cgcggaacat cacgaacgtc ggcgggcgtc gcctccatca cacgcgcgat ctgcaagttg
     3781 aattcgcgcc acaaggaaag tagcgattcc cacggagcgt cggcgtaacg tccgagtgat
     3841 acccacttct cctggtcgta gccggggaag atcagatcgt cggtgaagtt ggcgcggacg
     3901 aagcgctggt ggttgttcga ggcggaatcg atcaggtggc cgatgatctg tttgggggac
     3961 cacttgccgg gggcggtcgg tttcgcggca tcggcgtcgg agatggcaac caagtggggt
     4021 gacgcggatg cgattgcggc gcgaaggcgc tcttcgggca gcatcactgc gccagctcgt
     4081 acgacgcctg gatccacttg ctgacctgat cgtcgacttc ttccggcgcg gcgagccgca
     4141 cctcgaagtg ccacctgttt gcggacgtct gttccgtttt gtggacgcgg cggtgtttaa
     4201 tgtcgcggtc ggacttgagc gtcaggatca gcgcatcctt gcgcgtctgc acgcccgcga
     4261 aggcgacgcg gcggttgaga tggatcgatg tcttcttcgg atcctgctcg accgggccca
     4321 gcgccgatga agcgttgacg atggtgtcgt agatcctgcg cacgtgatcc gcgcggccgg
     4381 agaagtgctg ttccacggtc ggatcgggca tggggaaatg atagcgctct gaagcgatga
     4441 agatgtaggc gggacgcctg cggtcccaag gcgtctcgcg aaatctaccc gatgtagaat
     4501 ggcccgcccg atgcatgcct gaacgggcaa atcggtcggc ttctccagga caggagacta
     4561 catgagcgct gtcgaaaagg attacatcgc cccggttccc gcaccggccc cgtttgagcc
     4621 ttacatcccg gcagcaacga acatgcggga gctgacgccg ttgccgatca tcgtcggcac
     4681 cattctcggc atgatcttcg gcgcggcgtc cctctacctc gttttaaagg tcggcctgac
     4741 ggtcagcgcc tccataccgg tcgccgtcct ctccatcacc ttcttccgga tactctcgaa
     4801 gctcggcgga cgcgacgcca ccatcctcga gaacaacatc gttcagacgg ccggctcggc
     4861 cggtgagtcg attgcgttcg gcgtcggcgt gaccatgccg gccatcctga ttctcggctt
     4921 tgacctcgag ttcacgcgcg tgatgctcgt cgccgtcctc ggcggtctcc tcggcatcct
     4981 gatgatgatc ccgctgcgcc gcgccctcat cgtcgcgcaa cacggcactc tcaagtaccc
     5041 cgaaggcact gcgtgcgccg aggtgctgaa ggctggggcg tcggaggagt cgatcgcggc
     5101 cgcctcggat agcgccaaag cagagatggc aaagctcggc ggccaggcca cgagtgcgaa
     5161 aaccatcttt gccggcttcg gtatcggttt catctacaaa gcaatgaacg tcgccttccg
     5221 ctggtggaag gacacgcccg agcgtgtatt cggagcgccg ttcgcggcgg gctctgtctc
     5281 cgccgagatc tcgcctgagc tcctcggcgt cggctacatc atcgggccgc ggatcgcctc
     5341 gatcatgtgc gccggcggtg tgctcgccta tctggtgctc atccctgcaa tcaagttctt
     5401 cggcgcaggc cttgcggcgc cgctcgcacc cgaagcaacg cggctgatca aggacatgtc
     5461 gccgaacgcg atccgcagcg cctacatcct ttacatcgga gcgggcgccg tggcggcggg
     5521 cgggatcatc tcgatgctcc gttcgctccc gacgatctgg cacggtctga aggaagggct
     5581 gaaggacatg cgtggcggca gtgccgcgcg cgaggggatg gtccgcacgg accgcgacct
     5641 ttcgatgaag ctcgtgctca tcggcagctt cgcgctcgtg gccatcatcg cactggccag
     5701 cccgctgtac cacggcggca ccggcgtgat ggcgcgcgtg atggcagcga tcctgatcgt
     5761 cgtcctcggc ttcctcttcg tcaccgtctc atcgcggctc accggcgaga tcggctcgtc
     5821 gtcgaatccg atctccggca tgaccgtggc cacactgctg atcacctgcc tcgtcttcct
     5881 cgtgatcggc tggaccggtt cgacgtacta cgtcacggcg ctctcgatcg gcgcgatcgt
     5941 ctgcatcgcc tcgtcgaacg gcggcaccac atcacaggac ctcaagaccg gctacctcgt
     6001 cggctcgacg ccgaagtatc agcagatcgc catcctcatc ggcgcacttg cctcggcgct
     6061 cgtgctcgga ccgttgctcc tcatgctcaa caacaacggc agcgtagtgc tgccgcgcat
     6121 cagctccgaa ccgtcgaccg gcgaagaggg cgcgccgctc aagcaggtcg agaactgggc
     6181 acccggcctg cacgccgaac cgtcgatcct ggcgacgttg tctcacaaga gttacgaagg
     6241 gaaggactac ctcgtctggc actacacacc cgcgggggga gcatccgcgc aggagctcct
     6301 cgtcgacccg agcggcaccc cgcagtacct cgtcgacccg gcgatcaacg gcacgcacaa
     6361 gttcctggcc aacggcaaac tggcgccgaa gaagttcgac gcaccgaagg ccacgctgat
     6421 gtcgtacatc atcaaaggca tcctcaaccg gcagctcccg tggggtctcg tgctcctcgg
     6481 cgtgatgatc tcgctggtac tggagatgtc cggcatcccc tcgctcgcgt tcgcggtcgg
     6541 cgtttatctg ccgctgtcgt catcgagtcc gatcttcatc ggaggcatga tccgctggtt
     6601 gtcggacctc tatgttcgta agaagtatcg cgacgcgaac ctcacggaag agcagctcgt
     6661 ggccgaaggc gacaagagcc ccggcgtgct gatggcctcc ggctatatcg ccggcggcgc
     6721 gatcgccggg attctgatcg ccttcatcgc aggcctgcag gacaaggtgg catgggtgac
     6781 gaacttcaac gccagcctcg acaggttcgc cgacaagaat ccgttcgttt ccggcgcaca
     6841 ctccgacctc ctgtcctgca tcccgttcgt tttcctgatc ctgcttctgt acctcgtcgg
     6901 gcgcgagatt ctgatggcgc cgaaaacgcg tagcacctga tccatcaatc aacgcttgat
     6961 ttgcgaacgg cggcctttcg aggccgccgt ttttcgttaa cgcggtatcc tttgccggtc
     7021 caatggaatc tcttggctgc gtggtttttc tcgtcgcggt ctttgcgctg atcacgttcg
     7081 tggtggcgat ccgtgcgtcc ggccgtgcag gtgaggcgac cagagaagtt gccgaattga
     7141 agcggcgcct cgagcgcatg tcgcagcggg tctcgcacgc ggagagtgcc gcggccggtt
     7201 ttgcggatga ggaccttcgg catgcgccgg ccgccggggg tgttggcaag gttccggagc
     7261 cgatgccggc atggaccggc gcccccgttc gcgacgaaat cgcgcttctt cccgaggaga
     7321 tgcagccgcc cgcgccgacc cttcctgagc cggctccgat cgttgttcca cccgtcgccg
     7381 ccttcaccgc agaggcgcct gaaccggttg cagtcctcga cgaagcgatt tccgagcccg
     7441 aacctccgag ggaaataccg cccccacctc cgcccacacc ccacccccca cgccccgcac
     7501 ccccaccaaa gagacccttc gactgggagt cgctcatcgg cgtgaagctc ttctcgtgga
     7561 ttgccggaat cgcgctggtc ctggcggcgc tcttcttcct cagttactcc gtcgaacatg
     7621 gctggctcag tccgccggtg cgcgcgtcgc tcggcctgct gacgggagtg gcgctgctac
     7681 tcgtgtgcga gctgcgtgtg gcgcgcaact acgccttcac ggcgaatgcg atggacggcg
     7741 ccggcatcgc gatcctctac gccacactct tcgccatcca cgcgctctgg catctgctgc
     7801 cggctgctgc cgtcttcgcg ctcatgctgc tcgtgactgc gatcgcggtc atgctctcga
     7861 tccgccgcga ctccgtcttc atcgcgctgc tcggacttgt cggcggcttc gccacgccgg
     7921 cgttgctctc gaccggtgag aacaaaccga tcgccctttt ctcctacctg ctgttgctga
     7981 acgtcggctt gatgtgggtg gccatccggc ggcgctggcc gctcctgaca gcgctgagcg
     8041 tcatcttcac tgtgatctat gaatggggat ggatcggcaa gttcctgacc ggttcgcagc
     8101 ttcctctggc cgtcgcgatc ttcaccgttt tcgccgccgc tgctgcctcc tcgctatgga
     8161 tgcgccggcg cgccgacgcc aagcagggaa gttttgacct cgtcgccatt tcctcagccg
     8221 gtctgccgct tctttttgcg atctatgtcg ccgcagtggc gaactacggc gtgcagtaca
     8281 acgttctctt cacgttcctc ttgctgatca cctcggggct ttccgtgatc gcgatcgtcc
     8341 ggggtcctgc ctggctgcac ctcctcggcg gaggtacgat cgccctggtt ctcctcgtct
     8401 ggcgcgtgtc gtcctacaca ccgcaggcat ggccggcgat cctggcgtgg gtcgccggat
     8461 tcgtcgtcgt tcaactcgtg ttgtcgcact tcgccccaaa cccgcgagcg gttctcgtcc
     8521 cgctcctact cctgattctg ccgactctcg cggcgacgcc gattcccgca gccgcgcctc
     8581 tgcttctctt cggcgcgctt ttcgtgctcg tcgcgctcat cgccgcctac gccttcatgc
     8641 accacgccgg cgaagcgtac gcagtgggag cgttcctggc catcgtgacc gagggcgtct
     8701 ggtccgctca aagcatcacg ccggtgaatc tgacgaccgc gcttctcatt tacgccgctt
     8761 tcgcactgct cttcatcggt atcccggtca tcggccgccg gctcggccgc gagatcgtct
     8821 cgccgcggac gatgctcggc cttcttctcg gctcgatcgc gattctcttc ttcctcgcga
     8881 ctggaagcgt ggccacgtat tcactcctgg cgttggcaat tctcctcgtc atcatcaacg
     8941 cgggtgcgat cgccgaggcg cggaagaagg agcaagcggt gctggcggct gcggcgatcg
     9001 tcctttcgtg gaccgttatc accacctggt gcgcctcggc gatgactgca gcgaacctcg
     9061 tcgcggcatt gtccgtgatc ggcggcttcg cgctgctcgc tgtcggcggc ggcgtctggc
     9121 tgagccgcgg ggacgacgag gacgccggga ctacgtatct cggactgatc ggacatctct
     9181 tccttatcgt catcgcgaca caacccgcgc tggcgtttcc gccatggccg ttgttcgcgg
     9241 tgttgtttgt gctcgatctc gccatcggcg tcgccgcaat ctacctgcgc cgtgccagtc
     9301 tcatggccgc tgcgatggcc ggctcgcaga tcgtgctgat ggcgtgggca gcgaatacga
     9361 aagcggcgcc atggccggtt gtcgcaatcg gcgctgccat cgcggtctgc gcgatggcgt
     9421 tggtctggtt caggatcgac cggcgtttcg ccatcgccgc gctgatcgca ctcttcctcg
     9481 gcgacgtcgt tctcatcatc gccggcttca acgcgggcac tccgctcttc gcctcgctgc
     9541 tggccgcgca tctggcgatc gggatcgcga ttctcgccgt gacgtggctg accgagtggc
     9601 acgagccggc cgtggtcgca gtggctttgc tggcggtcgc caccgcgctt gctcgcacgt
     9661 cgacgcccgg aacgcgcctc acgttcgccg gggcaatcta cgcgatcttc atcatctatc
     9721 cgctcctact tggcggcaga gcgaagcgct cgctgtatcc gtatctcgcc gccgtactgg
     9781 cgagtgtgcc gttcttcatc gtggcgcgcg acgcggtacg cgacgccggc tacaactacg
     9841 caatcggcgt tctacctctg ttccaggcgg cgctgatgct tctccttctg tggcgtttgc
     9901 tgcgcgccga gccagcaacc gagcgtttgc tttcgcgatt ggcgctcgta gctggggcgg
     9961 cgctcgcctt catcaccgtc gccattccgc tgcagcttgc aaggcagtgg atcacgatcg
    10021 cgtgggcact cgaagcggcg gcgctgatct ggctcttccg ccggatcgcg caccgcggtc
    10081 tgctgatctg gtcgggcgca ctcttcgccg cggtcttcgt gcggttgctc ttcggcgacg
    10141 acgtcttcgt gcacaccgcc agccggctgc cgatcgtcaa ctggtacctc tacacgtacg
    10201 tcgtgagcgc agcggcattc tttgccgcgg cgcgcctact gcccgattcc gaggcgaagc
    10261 agtacccgtt cgcgcggccg gttctgagct ccggcggcac gatcctgctg ttcttcctcg
    10321 tcaacatcga gatcgctgat ttctactcga ccggttcggt gctgacgttc aactttttct
    10381 catcgtcgct ggcgcaggat ctgagctaca cgatcgcctg ggcggtcttc gccgtggcaa
    10441 tgctgatcgc cggccttttt ctgcacagcc gcgcgacgcg tattgccgcg atcatcctgt
    10501 tgctcgtcac cgtgctgaag tgcttcctgc acgatctggc gcgcctcggc gggctttatc
    10561 gcgtcggctc actcctcgga ctgactatct cgttgcttct cgtcggcgtg ttgttgcaga
    10621 agttcgtgat ccggaaagca gcgcctccga ccggggaggc agcgtgatcg ctgcggcgtt
    10681 tctgctggcg gcactgacct tgcccgaagt gccgtcgggt gcgccggtcg accgtggcga
    10741 gtttctctac gcacggcccc tcggcaccat cccagcaggc gtcgtctcac tcacgctcga
    10801 tgctcacgtt ctgctgcgga gccacgcgct gcaggacgtc cggatcgtcg acgcgaaagg
    10861 caatcaggtg ccttacctcg tcattccgct gacggcgccc acaatcgtct cgcttgcgat
    10921 tccacccgcg gcccgcgagg gcaaacagtc tgtctaccgg ctgacacttc cgtacgagtc
    10981 gttgcccaat ggcgcggagc tggagctgac taccagcgcc agcgtgttcg aacgcgatgt
    11041 cgttctccgc cgggcgctcg atgagagccg gggccgcgag gccgaggtga ttgatcgtct
    11101 cagttggaaa agcatgcctt cgatgcgagc cagtctcacc ggtgggcgcg cgatcgaagt
    11161 ggtcgtcgat gagggtgaca acgcgccgtt accgatcgtc tccgcgaagc tgcggctgcc
    11221 cggtttcgct ctccgattcg tgagccccgg cacgccgttg acgctgctct acggtaattc
    11281 gtcgatctcc gcgccacgct acgatctcgc gctcatttca tcgcagcttg ttggacagcc
    11341 ggcgcgcgcg atcgcgctca tcggagaacc ctcaaccgag ccgccggcga aacatcggga
    11401 gtcacgcctt ttcgggatcg cgatcggcgt ggcggccgcc gtcctgctga tcgtgctggg
    11461 acgcctcgtc agcgcgcagc ggtcctgaag aaccgagtgc gctcggcgag gctccatgtc
    11521 gtggcgacgc ggcgcgccgt ccagcgaggg atcgagcgcg caagccagag ataggcgacc
    11581 agcatcacgt tcaaaccgac cacgacagtc agtgaatcgc cgcggtcgag aaaggggacg
    11641 tgtgcacccg gccagcgaag aaaaagcccg tcgacgcaga cgaggagcag gaaatcgacg
    11701 gtgtcgcgaa cgattgcgcg gcggcgtgcc cttgcgacga cgacacgcga atcgatgacg
    11761 acgggagggg gtacggcggc gtctgaaacg ccgggtagca gagtgcgtcg aacgggcttc
    11821 tccatcgcaa tgtgttcgac gtttggggga ggtttgttat tccaaataat cgttatgcgg
    11881 gagtcggaga gacgaggggg tcgggggtcg gaggggaggt tggtgggttg cctgtgttgc
    11941 agggtggctg tgttaactaa cgtcaacgtg atcagcaagt taaatgagaa ggtggtcgag
    12001 tctcaacatc ctggtctcgc caccacatcc acctggccaa tctaccgagt gctcctggtc
    12061 ctcaaccaca ttcacctggt cagtccacca ggtgctcctg atccgcaacc acattcacct
    12121 ggtcagtcca ccaggtgctc cttatccgca accgcattca cctggtcagt cgaccaggtg
    12181 ctcctggtcc gcaaccacat tcatctggtc agtcgatcag gtgcttctgg tccgcaacct
    12241 catcgacgtg
//
LOCUS       DATLVW010000110        10495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_130282, whole genome shotgun sequence.
ACCESSION   DATLVW010000110 DATLVW010000000
VERSION     DATLVW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130282"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VNN25_03720"
     CDS             complement(<1..331)
                     /locus_tag="VNN25_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90665.1"
                     /translation="MPHSYPQEAVLRDGRKVLIRPFAAKDVDALYEFFQHLPVAYRRF
                     AWDPIENRSLIESWGRDIDYAKVLPLLALDGNNRIVADASLHRRQGGPLRLTGRIKWM
                     IDPAFRGV"
     gene            complement(377..1027)
                     /locus_tag="VNN25_03725"
     CDS             complement(377..1027)
                     /locus_tag="VNN25_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90666.1"
                     /translation="MFLSWILYCGFAAAFSGAIATLPRRTRRKGAWLFATGIASALIA
                     LFWPAAEEHADGVHAHIDEIMPRWQFEEHHEIQINAPPERIWAAIRDVTPREIRMFQT
                     LTAIRRFGRRSGENILNAPDTKPILDVATHSGFHVLVDDPPRELVLGTHVSPQALAVM
                     NFRLEGTHLTTETRVFGQTDEARRSFAVYWRLIRPGSGIIRRSWLEAIKRRAESAR"
     gene            complement(1343..1774)
                     /locus_tag="VNN25_03730"
     CDS             complement(1343..1774)
                     /locus_tag="VNN25_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90667.1"
                     /translation="MARSDEERLLKAIKENREALTSLLERARSHWEYEDPVYRFYHGS
                     FKVFSVQHLTLAIVSALRALLPERELNKQFLEILSEGTGKVFTTAVNADWDHSTRPLL
                     EAFFHARYFLEMAVKYGDELEEPTNLLPSGWAGLLYLYNLR"
     gene            complement(1780..4863)
                     /locus_tag="VNN25_03735"
     CDS             complement(1780..4863)
                     /locus_tag="VNN25_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH90668.1"
                     /translation="MKLTDLFIKRPVLAIVVNLVILIAGLQAIRSLSVRQFPRSDMAN
                     VVVTTAYVGANADLVRGFITGPLERSIAGADGIDYLESSSAQGISTITVHLKLNFDTN
                     AALTQIQSKVAQVRNQLPPEAEAPVIDLQTVDSQFAAAYLGFSSPDLDQNQITDYLVR
                     VVQPKLSAISGVQKAQIFGDRTFAMRVWLDPAKMASLGISPSQVNSALAANNYLSALG
                     QTKGSMTSVNLVANTNLQTADEFKKLVVKEDKGTIVRLGDIADVVLGAENYDSEVRFN
                     GQTAVFIGIYVLPTANTLDVMKQVREALPEIQAQLPAGMKAGVPYDSSAYIQEAIDEV
                     LKTLTETLLIVILVIFLFLGSARGLIIPVVAIPISLVGAVALMLAFGFTINLLTLLAV
                     VLSVGLVVDDAIVMVENIERHLHMGKTPVQAALDAARELVAPIIAMTITLASVYTPVA
                     LQGGLTGSLFREFAFTLAGAVLISGVVALTLSPMMGSKLLRAGDTEKGFAGMINRHFD
                     RLRGFYARLLTGTLNFRPVVITLWIIVVLLIFPFYMFSQKELAPAEDQSFLFAILQAS
                     ANNTIEQTRLFADEVEKVYRSIPEKEATFQVIFPTGGFGGMVTKPYKERKRGIPEIQQ
                     EAAAKSASIPGIRVIQLVPPSLPGNGGGFPVDLVLVSTEEPARIAEFANQLMQKAFTS
                     GKFMFADTDLKFDQPQTRVVFDRDKLRSQGVDLSQAGRDLSVLLGGNFVNRFSIQGRS
                     YKVIPQVKRADRLTPDQLSQMYISGAGGKLVPLSTFATLESTTEPRELKRFQQLNAVH
                     VQGAIPPGVSIDSALTYLEDEAHKILPRGYTLDYAGESRQLRIEGSRFLGVFFLSAIL
                     IYLVLAAQFESFRDPFIILAGSVPLALSGSLLFSFLGLTSINIFSQVGLITLVGLVSR
                     NGILITQFANHLQEEGRDKLHAVIEAAGTRLRPILMTTAATVMGHLPLVFARGPGAGA
                     RNSIGIMLVTGMIIGTGFTLFVVPSIYVLVAKTHKRIDAVETEPEPGLEPEPATV"
     gene            complement(4951..6069)
                     /locus_tag="VNN25_03740"
     CDS             complement(4951..6069)
                     /locus_tag="VNN25_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH90669.1"
                     /translation="MAKRMIVMLVVVIAFVAAIGGYKYLQIKKAMSQSYTPPPEAVTT
                     TVAKQETWDTTISAIGTVVAVNGVTLSADLPGVVDQISFDSGRMVGKGDVLLRQDTKQ
                     ERAQLASAEAQLGLTRVQLARNRSLQAAGIVAKESLDQAAAGFTQAEAAINEIKATIE
                     RKTIRAPFAGVLGIRQVNDGQSLASGAPIVSLQALQPVYVNFSVPQREIGLLHTGGAI
                     GVTSDSFPGTETGKINAFDAVVNEDTRNIQVQAVFGNASRKLRPGMYVEAQLAQGART
                     PVIALPVSAVSYAPFGDSVFIVEDVKGPDGKTFRGVRQQFVKLGPARGDLVAVLTGVK
                     PGEEVVTSGVFKLRPGAAVVVNNKIQPGSSETPKPGNS"
     gene            complement(6344..6898)
                     /locus_tag="VNN25_03745"
     CDS             complement(6344..6898)
                     /locus_tag="VNN25_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HXH90670.1"
                     /translation="MKRQLLLLVGIVSIATLPLPVGVRIAEGAQQQVGVTLTYDPAYV
                     SLRYPGGDVPLSRGVCADVVVRAFRVAGIDLQRAVHEDMLGAFKRYPPLWGLSHPDAN
                     IDHRRVPNLMKFFERHGKTASGGFLPGDVVAWRLPGGLLHIGVVSAEVASQTKHPLMI
                     HNIGNGAQKEDVLFAYTIIGHYRW"
     gene            complement(6904..7359)
                     /locus_tag="VNN25_03750"
     CDS             complement(6904..7359)
                     /locus_tag="VNN25_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90671.1"
                     /translation="MKRIAAIVAIAAVAGYWYYQTSHAPVNAYKVFAEEILHRRYDKA
                     AAMTDGLTAAQLAQSGTQEKIGAGPAMFQTLFPSRFTIESQERGSDGTTTIKATQQVL
                     FNPPGVESVMRPAMMALMKQTVALRKKDGAWKVVSFENTFGNMETMSAR"
     gene            complement(7370..7819)
                     /locus_tag="VNN25_03755"
     CDS             complement(7370..7819)
                     /locus_tag="VNN25_03755"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH90672.1"
                     /translation="MVTPDVVLTARRHAFACLFARLVLGLIFFMAGVYKVFTLTPVGH
                     VQKWFLPFRDTFLPTWSLWAVGLAIPFIELIGGALLLIGWRVYEALLALGVVLVIVTF
                     GHLLHEPLYPFHEHVIPRLALLLFLLVMPRESDRFSIDYWIATRRQS"
     gene            complement(7851..8216)
                     /locus_tag="VNN25_03760"
     CDS             complement(7851..8216)
                     /locus_tag="VNN25_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90673.1"
                     /translation="MRRTTLVLLAVSILFVSVSLEARPGWSGTYFVNGTNPGVGVYKG
                     TLNITPRGDVYDVRWSIAGLRYTGVGIVVNETLSVAYTDANHSFIGVASYTREGGTLY
                     GHWAVAGGAVMGTETATRR"
     gene            complement(8282..8560)
                     /locus_tag="VNN25_03765"
     CDS             complement(8282..8560)
                     /locus_tag="VNN25_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HXH90674.1"
                     /translation="MCRNIKTLFNFEPPATEEEIRDASLQFVRKLCGFTKPSKANQAA
                     FDLAVEETALVAQKLFASLVTTAEPHDRETEAAKARARSAARFGRAEA"
     gene            complement(8656..9090)
                     /locus_tag="VNN25_03770"
     CDS             complement(8656..9090)
                     /locus_tag="VNN25_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90675.1"
                     /translation="MIDRPLRSELMNLLRELSEGSASRDQFRAPLSRSKDLAVREIAE
                     QAWLLCRDLPEPGSPDRKKRLREARGEISRWTLFLDGDREYEWPVLPKWARVLGFFPS
                     VLTFGLFWAPYRWWFKRSGDSQVWPFIDEKQFEEARRRGGRR"
     gene            complement(9087..9746)
                     /locus_tag="VNN25_03775"
     CDS             complement(9087..9746)
                     /locus_tag="VNN25_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXH90676.1"
                     /translation="MPTPMKVVSVNVGLPREVISRGEAVLTSIFKEPVAGRVRVETLN
                     LVGDRQSDLTVHGGAEKAVYVYPSEHYAFWREELPGVDLPWGSFGENLTVEGLAEDRV
                     RIGDEIAIGSAVFVVTQPRMPCYKLGIRFGRMDMVKRFLKSGRSGFYLSVSSEGELGA
                     GDLITLTPSQTPSITVAEFVSIYATNGRDKELLRRATLVPAIPEGWRDYLHERLREDA
                     S"
     gene            9849..>10495
                     /locus_tag="VNN25_03780"
     CDS             9849..>10495
                     /locus_tag="VNN25_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90677.1"
                     /translation="MRKTQLVALVLFASLTTLQAQPYQRKTEATPSQPIAGLTQSLWN
                     AYDRAIYDSSVYQRWNLRLLRPLTPDAAGQVLVATATSREGNVGETILVPAAGIWVTG
                     VPEVQTICRNFHGDVTMQVRQLLGLPPDADAPRVLTFKVNMSDLFRPSPDDTITTTYP
                     CQYTAGSPPPLDCGNVFPSTTTPAHYQWIATQSFLLHAVPNGYPWTHLGYTYNWAP"
BASE COUNT         1993 a         3200 c         3341 g         1961 t
ORIGIN      
        1 cgacgccgcg gaacgccgga tcgatcatcc atttgatgcg gccggtgagg cggagcgggc
       61 cgccttgtct gcggtgcagc gaggcgtcag cgacgatgcg gttgttgccg tccagcgcca
      121 ggagcgggag gactttcgcg tagtcgatgt cgcggcccca gctctcgatc agacttcgat
      181 tctcgatcgg atcccaggcg aaacggcggt aggcgaccgg gaggtgctgg aagaattcgt
      241 acagtgcatc gacgtccttc gcggcgaagg ggcggatgag aactttgcgg ccgtctcgca
      301 ggacggcctc ctgcggatag ctgtgcggca tgggcgaacg atatatcaga tcagatgatg
      361 gaaacgactg tgcctgtcat cgtgccgact ccgcccgccg cttgatcgct tcgagccagc
      421 tccggcgaat gatgccgctg ccggggcgga tgagacgcca gtagacggcg aacgaccggc
      481 gcgcttcgtc ggtctggcca aacacgcgcg tttcggtcgt gaggtgcgtg ccctcgaggc
      541 ggaagttcat cacagcgagc gcttgcggcg agacgtgcgt gccgaggaca agctcgcgcg
      601 gagggtcgtc gacgagcacg tgaaaaccgg agtgcgtggc cacatcgagg atgggctttg
      661 tgtcgggagc gttgaggatg ttctcgccac tgcggcggcc gaagcggcgg atggcggtca
      721 gcgtttggaa catgcggatc tcgcgcggcg tgacgtcacg gatggctgcc cagatccgtt
      781 ccggcggagc gttgatctgg atttcatgat gctcttcgaa ttgccagcgc ggcatgatct
      841 cgtcaatgtg tgcgtggaca ccgtccgcat gctcctcggc ggccggccag aagagggcga
      901 tgagcgcgga ggcgatgccg gtagcgaaga gccacgcgcc tttgcgtcgc gtgcggcgcg
      961 ggagtgttgc gatcgcgccg ctgaaggctg ccgcgaagcc gcaatagaga atccaggaga
     1021 ggaacatcgt tggggaagtg taatggcgct cggaaatccg cgctgatgtt gccattcacg
     1081 atgacaagcc cccattggtg tcatcctgag acagccggag ccgcccgctc ggatcgcgat
     1141 gtttgcgcag tgcgtgctcg gcggcgtagg acggcgaagg atctccagtt gccgcgcacg
     1201 tggcctgcgc ctcgtaattt gagatccttc gacgtcctac gccgccacat ctttcagcgc
     1261 gacggctcga gtacggatgg cggctccggc agtctcagga tgacacttca attacagttc
     1321 gtctcggaat gcagaccgac aatcatcgca agttgtagag atagagcaat cctgcccatc
     1381 cgctgggaag caggttcgtt ggctcttcga gctcgtcgcc gtacttcact gccatttcga
     1441 gaaagtagcg ggcgtggaag aacgcttcca acagcggccg tgtcgaatga tcccaatcag
     1501 cgttcacggc agttgtgaag actttgcctg tgccctcgct caggatttcg agaaactgtt
     1561 tgttgagctc ccgctcaggc aagagtgctc gcagcgccga gacgatcgcg agcgtcagat
     1621 gctgcacgct gaagaccttg aaactgccgt gatagaagcg atagacagga tcttcgtact
     1681 cccaatggct ccgtgcgcgt tcgagcagcg acgtcaatgc ctcgcgattc tctttgatcg
     1741 ctttgagcag gcgctcttcg tcacttcgag ccattctccc tacaccgttg ccggctccgg
     1801 ttccagtcca ggctccggct ccgtctcgac cgcatcgatt cgtttgtgcg tctttgctac
     1861 gagcacgtag atcgacggga cgacgaagag cgtgaagccg gtgccgatga tcatgccggt
     1921 gacgagcatg atgccgatgg agttgcgggc gccggcgccg gggccgcggg cgaagactag
     1981 cgggaggtgg cccatcaccg tcgcggccgt cgtcattaga atcgggcgaa ggcgggtgcc
     2041 ggcggcttcg atcaccgcgt gcagtttgtc gcgtccttct tcctgcaggt gattggcgaa
     2101 ctgggtgatg aggatgccgt tgcgcgagac gagtccgacc agcgtgatca ggcccacctg
     2161 gctgaagatg ttgatcgacg tcaggccgag gaatgagaag agcagcgaac cggagagggc
     2221 gagcgggacg gagccggcca ggatgatgaa ggggtcgcgg aagctctcga actgcgcggc
     2281 cagcacgagg tagatcagga tcgccgagag gaagaagacg ccgaggaagc ggctgccttc
     2341 gatgcgcaac tggcgcgatt cgccggcgta gtcgagcgtg tagccgcgcg gcaggatctt
     2401 gtgcgcttcg tcctcgaggt acgtcagcgc gctgtcgatg gagacgccgg gcgggatggc
     2461 gccttgcacg tgtacggcat tgagttgctg gaagcgcttc agctcgcgcg gctcggtcgt
     2521 ggactccagc gtggcaaacg tcgagagcgg aacgagtttg ccgccggcgc cgctgatgta
     2581 catctgcgag agttgatcgg gcgtcagccg gtccgcgcgt ttgacctgcg gaatcacttt
     2641 gtagctgcgc ccctgaatac tgaaccggtt gacgaagttg ccgccgagga ggacggagag
     2701 gtcgcgtccg gcctgcgaga gatcgacgcc ctgggagcgg agtttgtcgc gatcgaagac
     2761 gacacgtgtt tgcggttgat cgaacttcag atcggtgtcg gcgaacatga acttgccgct
     2821 cgtgaacgcc ttctgcatca attgattcgc gaactccgcg atgcgcgccg gctcctcggt
     2881 cgacaccagc acgaggtcaa ccgggaaacc tccgccgtta cccggcagcg acggcggtac
     2941 gagctggatc acgcggatgc ccggaatgct cgccgatttc gcggcggcct cctgctgaat
     3001 ctccggtatc ccgcgcttcc gttccttgta tggcttcgtg accatgccgc cgaagccgcc
     3061 ggtgggaaag atgacttgaa acgtggcctc tttctccggg atcgagcggt acaccttttc
     3121 gacctcgtcg gcgaacaacc tcgtctgttc gatcgtgttg ttcgcagacg cctgaaggat
     3181 cgcgaagagg aagctctggt cctcggccgg cgccagttcc ttctgcgaga acatgtagaa
     3241 cggaaagatc agcaggacca cgatgatcca gagcgtgatc acgacgggcc ggaagttcag
     3301 cgtgccggtc agcaggcgtg cgtagaaacc gcgcagcctg tcgaagtgcc ggttgatcat
     3361 gccggcgaag cctttctccg tgtcgcccgc gcgcagcagc ttcgatccca tcatcggcga
     3421 caaggtcagc gccacgacgc ccgagatgag caccgcgccg gccagcgtga aggcgaactc
     3481 gcggaagagc gatcctgtca aaccgccttg cagcgccacc ggcgtgtaca ccgaggcgag
     3541 cgtgatggtc atggcgatga tcggcgccac cagctcgcgc gcggcgtcga gcgccgcctg
     3601 caccggcgtc ttacccatgt gcagatgccg ctcgatgttc tcgaccatga cgatggcgtc
     3661 gtcgacgacg agaccgaccg ataagaccac agcgagcaac gtcagcagat tgatggtgaa
     3721 accgaaggcg agcatcaacg cgacggcgcc gaccagcgag atcggaatgg cgacgaccgg
     3781 aatgatcagg ccgcgcgcgg atccgaggaa aaggaagatc acgaggatga cgatgagcag
     3841 cgtttccgtg agcgtcttga gcacctcgtc gatcgcttcc tggatgtatg ccgaggagtc
     3901 gtacggtacg ccggccttca tccccgcggg gagctgcgcc tggatctcgg gaagcgcttc
     3961 gcgcacctgc ttcatcacgt cgagcgtgtt ggcggtgggc aggacgtaga tgccgatgaa
     4021 gacggcggtc tgaccgttga aacgcacctc ggagtcgtag ttttcagcgc cgaggacgac
     4081 gtcggcgatg tcgccgaggc ggacgatcgt ccctttgtct tccttcacca cgagcttctt
     4141 gaactcgtcg gcggtctgca gattggtgtt cgcgacgagg ttgaccgagg tcatcgaacc
     4201 cttcgtctga ccgagcgcgg aaaggtagtt gttcgctgcc agcgcgctgt tcacctgcga
     4261 cggcgagatg ccgagcgacg ccatcttggc gggatcgagc cacactcgca tcgcgaacgt
     4321 ccggtcgccg aagatctgcg ctttctgcac gccgctgatg gcggagagct tgggctgcac
     4381 gacgcggacg aggtagtcgg tgatctggtt ttgatcgagg tcgggggatg agaagccgag
     4441 gtaggcggcg gcgaactgcg agtcgacggt ctgcagatca atgaccggcg cctcggcttc
     4501 aggcggcaac tgattgcgca cctgcgcgac cttcgactga atctgcgtca acgctgcgtt
     4561 ggtgtcgaag ttgagcttga ggtgaacggt gatcgtgctg atgccctgcg cgctcgacga
     4621 ctcgagatag tcgatgccgt ccgcgccggc gatggagcgc tcgagcgggc cggtgatgaa
     4681 gccgcgcacg aggtcggcgt tcgcgccgac atacgccgtc gtgacgacga cgttggccat
     4741 gtcgctgcgc ggaaattgcc gcaccgacag cgatcggatc gcctgcaacc cggcgatgag
     4801 aatcaccaga ttgacgacaa tcgccaggac agggcgtttg atgaagaggt cggtgagttt
     4861 catgtgatta aagtcagagg gcagaaagca gaaggcagaa ggctgcgccg cttcatcctt
     4921 ttgccttcat ctttctgcct tctgccttcc ttaactgttc cccggcttcg gcgtctcgct
     4981 cgatcccggt tggatcttgt tgttgaccac tacggccgca ccgggccgca gtttgaatac
     5041 gcctgaagtg acgacttcct cgccgggctt gacgccggtg aggacggcca cgaggtcgcc
     5101 gcgcgcaggg ccgagcttca cgaactgctg gcgcacgccg cggaaggtct taccgtccgg
     5161 acctttcaca tcctcgacga tgaagacgga gtcgccgaag ggtgcgtaac tgaccgcgga
     5221 gaccggcagc gcgatgaccg gcgtgcgcgc accttgtgcc agttgcgcct cgacgtacat
     5281 ccccggccgc agtttgcgcg acgcgttacc gaagacggcc tgaacctgaa tgttgcgcgt
     5341 gtcttcgttg acgacggcgt cgaacgcgtt gatcttgcct gtctccgtgc cggggaacga
     5401 gtcggatgtg acgccgatcg cgccgccggt gtgcagcaat ccgatctcgc gctgagggac
     5461 gctgaagttg acgtagaccg gctgcaacgc ctgcagcgaa acgatcggcg cgccactggc
     5521 gagcgactgt ccgtcgttga cttgacggat gccaaggacg ccggcgaagg gcgcgcggat
     5581 tgtcttgcgc tcgatcgttg ccttgatctc gttgatcgcc gcctcggctt gcgtgaaccc
     5641 ggccgctgcc tgatcgagcg actctttcgc cacgattccg gccgcctgca gactccggtt
     5701 gcgcgcaagc tgcacccgcg tcaacccgag ttgcgcctcg gccgacgcga gctgcgcgcg
     5761 ttcctgcttc gtgtcctgcc ggagcagcac gtcacccttc cccaccatcc gccccgaatc
     5821 gaaggagatc tggtcgacga cgccgggaag gtcggcgctg agggtgacgc cgttgactgc
     5881 cacgactgtc ccgatggccg agatggtggt atcccacgtt tcctgcttcg ccacggtcgt
     5941 cgtcaccgct tcgggtggcg gggtatacga ctgcgacatc gctttcttga tctggaggta
     6001 tttgtagccg ccgatggctg ccacgaaggc aatcaccacg accagcatca cgatcatccg
     6061 tttcgccata cgactgattc cctcaactca tagaaccggc aaccggccgg tttgttacgg
     6121 cgggaaagaa tacaacggcg tttgcgggca attgaaaacg aacggtcaat ggagtgcagg
     6181 cgtctcgcct gcggccggcg ggcgtctcgc caggcgtctc gcctggcggt ggtttgccgc
     6241 aagggtcatt tcctctcagg gatcgagtct tacacgccga ggcgagacgc ctcgtcgccg
     6301 cgggcgagac gcctgcactc cttcgcgcga gggcggcgga aatctaccag cggtagtgtc
     6361 cgatgatcgt gtaggcgaag aggacgtctt ccttttgcgc gccgttgccg atgttgtgaa
     6421 tcatcaacgg atgcttcgtc tgcgacgcga cttcggcgct gacgacgccg atgtgcagca
     6481 gcccacctgg caggcgccag gcgaccacat ctccgggcag gaaccctccg cttgcggtct
     6541 tgccgtgccg ctcgaagaac ttcatcagat tcggcacgcg gcggtgatcg atgttcgcgt
     6601 cgggatggga aagcccccag agtggcggat agcgcttgaa cgctcccagc atgtcttcgt
     6661 gaaccgcgcg ctgcaggtcg atcccggcca cgcgaaacgc ccgcaccacg acatccgcgc
     6721 acacaccgcg gctgagagga acgtcgccac ccggataccg gagcgaaacg taggcagggt
     6781 cgtaggtcag cgtcacgccg acctgttgct gcgccccctc cgcgatgcgc acgccgaccg
     6841 gcagtggcag cgtggcgatc gagacgatgc caaccaggag gaggagctgt cttttcacat
     6901 tcatcaccgc gccgacattg tctccatgtt tccgaacgta ttctcgaagg agaccacttt
     6961 ccacgcgccg tccttcttgc gcagcgctac ggtctgcttc atcagggcca tcatcgccgg
     7021 ccgcatgacg gactccacgc cgggcgggtt gaacaacact tgctgcgtcg ctttgattgt
     7081 agtcgtcccg tcgctgccgc gttcctgcga ttcgattgtg aaccgcgaag gaaacagcgt
     7141 ttgaaacatg gccggcccgg cgccgatctt ctcctgcgtt cctgactgcg ccagttgcgc
     7201 cgcagtgaga ccatccgtca ttgccgctgc cttgtcataa cgccgatgaa gtatctcctc
     7261 ggcaaagacc ttgtaggcgt tcacaggagc gtgcgaggtc tggtaatacc agtagccggc
     7321 cactgctgca atcgcgacga tggctgcgat tcgtttcatg attcggccct caagactgac
     7381 ggcgtgtcgc gatccaataa tcgatcgaga agcggtcgct ttcgcggggc atcacaagca
     7441 ggaacaaaag cagagcgagc cgcggaatga cgtgctcgtg aaacggatag agcggttcgt
     7501 gcagcaaatg gccgaacgtg acgatcacga gcacgacacc cagagccagc agcgcttcgt
     7561 aaacccgcca gccgatcagg agcaacgctc cgccaatgag ctcaatgaaa gggatggcca
     7621 gtccgaccgc ccacaacgac caggtgggaa gaaatgtgtc gcgaaacggc agaaaccatt
     7681 tctgcacatg acccaccggt gtcagcgtaa ataccttgta cacgccggcc atgaagaaga
     7741 tcaggccgag cacaagacgc gcgaagagac aggcaaaggc atgccgccgc gcggtcaaca
     7801 ccacgtccgg tgtcaccatc cgcaaccctc ccacgcggcg gcgagcaacg ttaccttcgc
     7861 gtcgcggttt cggtgcccat cactgcaccg ccggcgaccg cccaatgccc gtagagcgtg
     7921 ccgccctcgc gcgtgtagga ggcgacgccg atgaaactgt gattggcatc ggtgtacgcc
     7981 acggaaagcg tttcgttgac gacgatcccg acgccggtat atcgcaggcc ggcaatgctc
     8041 cagcgcacgt cgtaaacatc gccccgagga gtgatgttga gcgtgccctt gtagacgccg
     8101 acgccggggt tggttccatt gacgaaatag gttccgctcc agccgggcct ggcctcgagg
     8161 gacaccgaca caaacaggat ggagacagca aggagaacga gagtggtccg tcgcatggtc
     8221 atttcctcct ccaatcggag gaaatggtag cgcatcggct gggctcgcct gaagttttcc
     8281 gctacgcttc cgcgcgcccg aatcttgccg cggacctcgc gcgcgccttg gcggcttctg
     8341 tctcgcggtc gtgcggctcc gcggtcgtca cgagggaagc aaacaacttc tgcgcgacca
     8401 acgcggtctc ctcaaccgcg agatcaaacg cggcctggtt cgccttcgac ggcttcgtga
     8461 agccgcacag cttgcgcacg aactgcaggg aggcgtcgcg aatctcctcc tccgtcgccg
     8521 gaggctcgaa gttgaaaagg gtcttgatgt ttcggcacat ggggaggctc ctggttggaa
     8581 agtataggga aagtgagttt ttcacgcgaa ggcgcgaagg gggatgtctc ttcgcgtctt
     8641 cgcgtcttcg cgtgactacc gccgcccgcc ccttctccgt gcctcctcaa actgcttttc
     8701 atcgatgaac ggccacactt gcgagtcacc acttcgcttg aaccaccatc gataaggcgc
     8761 ccagaagagg ccgaaggtga gcacgctggg aaagaatccg aggacgcggg cccatttcgg
     8821 aaggacgggc cactcgtact ccctgtcgcc atccagaaag agagtccagc gcgagatttc
     8881 gccgcgcgct tcgcgaagcc gtttcttgcg gtccggggat cccggttcgg gaagatcgcg
     8941 gcagagcagc caggcctgct ccgcgatctc gcgaacggca agatccttcg atcgcgagag
     9001 tggtgcgcga aactgatccc ggctggcgct tccttccgac agctcgcgaa ggaggttcat
     9061 cagttcgctg cgaaggggcc ggtcaatcac gaagcatctt cccgaagccg ttcgtggaga
     9121 tagtcgcgcc acccttcagg aatcgccggg accagcgtag cccggcgcag cagctccttg
     9181 tccctgccgt tcgtagcgta gatgctgacg aattcagcga ccgtgatcga cggcgtctgc
     9241 gagggggtca acgtgatgag atctcctgcg cccagttcac cctcgctgga aaccgaaagg
     9301 tagaaaccgc tccggccgct cttgagaaag cgcttcacca tgtccatgcg gccgaagcga
     9361 attccgagct tgtagcaagg catgcgcggc tgcgtcacga cgaacactgc cgagccaatc
     9421 gcgatctcgt cgccgatgcg cacgcgatcc tcggccagac cttcgaccgt gaggttctcg
     9481 ccgaacgagc cccagggcag atcgacgccg ggcaactcct cgcgccagaa tgcatagtgc
     9541 tcggacggat aaacgtaaac cgccttctcc gcgccgccat gcacggtgag atccgactgc
     9601 cggtccccca ccagattcaa cgtttcgacg cgcacgcggc ccgcaaccgg ctctttgaag
     9661 atcgacgtca ggacggcctc gccgcgggag atgacctcgc gcggcaggcc cacgttcacg
     9721 gacacaacct tcatcggggt gggcatttcc ggcgaatcgt agcgcagtag cgagaacggt
     9781 tgacgctcgc agaacggttg ggctagcctt cgatacttaa ttcccggatc aaaggagtgg
     9841 ccacacgcat gcgaaagaca cagctcgtcg ccctcgtttt gtttgcctcg ctcacgacac
     9901 tccaggcgca gccatatcaa cgaaagactg aggcgacgcc gagtcagccg atcgccggcc
     9961 tgacgcaatc gctatggaac gcttacgacc gcgccattta cgattcatcg gtatatcagc
    10021 gatggaacct ccgtcttctt cgcccgctta cgcccgacgc agccgggcag gtgttggtcg
    10081 caacggccac ctctcgggag ggcaacgttg gcgagacgat tctggttccg gcagcaggaa
    10141 tctgggtcac tggcgtaccc gaggtgcaaa cgatctgtcg gaactttcac ggcgacgtga
    10201 cgatgcaggt gcgccaatta ctcggattgc cgccggacgc ggatgcgccg agggtgctca
    10261 cattcaaagt caatatgagc gatctcttcc ggccctctcc agacgacacc atcacgacga
    10321 cgtatccatg ccaatatacg gccggcagcc cgccaccgct ggactgtggc aatgttttcc
    10381 cttcgacgac gacaccggct cactatcaat ggatcgctac gcagtctttc ctgttgcacg
    10441 ccgtacccaa tggataccca tggacgcacc tcggctacac ctacaattgg gctcc
//
LOCUS       DATLVW010000111         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_130371, whole genome shotgun sequence.
ACCESSION   DATLVW010000111 DATLVW010000000
VERSION     DATLVW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130371"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VNN25_03785"
     CDS             complement(<1..488)
                     /locus_tag="VNN25_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90678.1"
                     /translation="MKKLLALAALLVAIAGRAEDSPLVKAAKENGGPKKKTTKKAITN
                     ADVKKSAVKSSAPATGAKAAPKQETPAVPMPPAIADQSKLVHARADAQKRADDAQVKV
                     NDLDKELDRLEQSYYAENDPNYRDKTIAARFEQTKLQLETARKELADARDALEKLQPK
                     QQ"
     gene            complement(485..868)
                     /locus_tag="VNN25_03790"
     CDS             complement(485..868)
                     /locus_tag="VNN25_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH90679.1"
                     /translation="MNDHLFDHLPVKRVSKPWGYELIFAKTDRYVGKILHINRGESLS
                     LQYHEIKEETLFVVAGDLKLTIEVDGDRREIMLRGGEAFHIPPRMIHRMEALEDTDLA
                     EVSTPELDDVVRLEDRYGRAGTSAP"
     gene            complement(871..>2661)
                     /gene="ptsP"
                     /locus_tag="VNN25_03795"
     CDS             complement(871..>2661)
                     /gene="ptsP"
                     /locus_tag="VNN25_03795"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXH90680.1"
                     /translation="MPTTTSPSRTSIRTYKGIGVSPGIAIGRAVIVERLEAAVYRVPI
                     RDDEVPSEVNRFLEAIEKTQSQLLDLKQKVKRSMGDEYASIFEAHAMMVSDPSFADKV
                     VQKIEAEQVNAEWALAEVQEELQARFRSFDDVYLRERVADVKDVADRVQKNLQGVSHH
                     DVSEITHDVIILADDLTPSDTIHFNRRPIVGFATEAGGRTSHTSIIAKSLFMPAVIGV
                     PRLTKIVDNDEMVILDGYEGLVLVNPTQAMIAEYQSRVIRHEEAERRLLENRDLVALT
                     KDDRRITLQANIELVEELKDAIRFGAEGIGLYRSEFLYISKSPLLPNEEEHFQLYRAL
                     AEATAPQWCVIRTFDLGGRKLAKEVIGSKENNPVLGLRGLRLCMKHRDMFRTQLRALL
                     RASAFGKIKIMFPVVTSVQELRQVKTLVRELRAELDAEGIAHNPELPIGIMIEVPSAA
                     IIADILATEADFFAIGTNDLIQYSLAIDRSNENVSYLYEPLHPALLRLLKMIIDAGHK
                     ANIPVSLCGEMGADPLFAIVLLGLGLEIFSMNPSSIPVIKNIVRSVRYRDCKRIAEVA
                     LTKKTAQEIEEFIIESVAMRFPEGLINKTP"
BASE COUNT          466 a          822 c          797 g          576 t
ORIGIN      
        1 tattgctgct tcggttgcag tttctccaac gcgtcacgcg cgtctgcgag ttccttcctg
       61 gcagtttcca attgcaactt cgtctgttcg aaccgcgcgg caatcgtctt gtcgcgatag
      121 ttcggatcgt tctccgcgta atacgactgc tccaggcgat cgagctcttt gtcgaggtcg
      181 ttgaccttga cttgcgcgtc atcggcacgc ttctgggcgt ccgcacgggc atgtacaagc
      241 ttgctctgat cggcgatggc tggcggcatg ggcacagcag gcgtctcctg tttcggagcc
      301 gccttcgctc cggtcgccgg cgccgagctc ttcaccgccg atttcttcac gtcggcgttg
      361 gtgatcgctt tcttggtcgt tttcttcttc ggtccgccgt tctctttcgc cgccttgacg
      421 agcggcgagt cctctgcgcg gccggcgatg gctacgagaa gagcggcgag agcgagaagc
      481 tttttcatgg ggctgatgtg ccggcgcggc cgtatcgatc ttcgaggcgg acgacatcgt
      541 cgagctcggg cgtcgagacc tccgcgaggt ccgtatcctc caacgcttcc atccgatgaa
      601 tcatccgcgg cggaatgtga aacgcctcgc cgccgcgcag catgatttcg cggcggtcac
      661 catcgacttc gatcgtcaac ttcagatcgc cagccacaac aaagagcgtc tcttctttga
      721 tttcgtggta ctgcaaactg aggctctcgc cgcggttgat gtgcaggatc ttgccgacgt
      781 accgatccgt cttcgcgaag atcaattcat atccccacgg tttggaaacg cgtttgacgg
      841 gcaggtgatc gaagagatga tcgttcattg tcatggcgtc ttgttgatga ggccctcggg
      901 gaaacgcatg gccacgctct cgatgatgaa ctcctcgatc tcctgcgcgg tcttcttcgt
      961 cagcgcaacc tcggcgatcc gcttgcaatc acggtagcga acggagcgca cgatgttctt
     1021 gatcaccggg atcgaggacg gattcatcga gaagatctcc aggccgaggc cgaggagcac
     1081 gatggcgaag agcggatcag cgcccatctc gccgcaaagc gagaccggga tgttggcctt
     1141 gtgcccggcg tcgatgatca tcttcaacaa ccgcaacagc gccggatgca gcggctcgta
     1201 gagatagctt acgttctcgt tcgagcgatc gatcgcaagc gagtactgaa tgaggtcgtt
     1261 cgtgccgatg gcgaagaagt ccgcctcggt cgccaggatg tcggcgatga tcgcggcgga
     1321 cggcacttcg atcatgatgc cgatcggcag ctcgggattg tgcgcaatac cctcggcgtc
     1381 gagctccgcg cgcagctcgc gaaccaacgt cttcacctgc cgcaactcct gcacgctggt
     1441 gacgacggga aacatgatct tgatcttccc gaacgccgag gcgcgcagca gagcgcgcaa
     1501 ctgcgtgcga aacatgtcgc gatgcttcat gcagaggcgc agcccacgca acccgagcac
     1561 cggattgttt tccttgctgc cgatgacttc cttggcgagc ttgcggccac cgaggtcgaa
     1621 ggttcgaatg acgcaccact gcggcgcggt agcctcggcc agcgcgcggt agagctggaa
     1681 gtgctcctct tcgttgggca gcagcgggct cttcgaaatg tagagaaact ccgaccgata
     1741 gagcccgatc ccctccgcgc cgaagcggat cgcgtccttc aactcttcga ccagctcgat
     1801 attcgcctgc agcgtgatcc gccggtcgtc cttcgtcagc gcgacaagat cgcgattctc
     1861 gagtagccgc cgctccgcct cttcgtgccg gatcactcgc gactggtact cggcgatcat
     1921 cgcctgcgtc gggttgacga gcacgaggcc ttcgtacccg tcgaggatca ccatctcgtc
     1981 gttgtcgacg atcttcgtca ggcgcggaac cccgatgacg gccggcatga acaatgactt
     2041 ggcgatgatc gacgtatgcg acgttcgccc ccctgcctcg gtcgcgaaac cgacgatcgg
     2101 ccggcgattg aaatgaatcg tgtccgacgg cgtgaggtcg tcggcaagga tgatcacgtc
     2161 gtgcgtgatc tccgagacat cgtgatggga aacgccctgc agattcttct gcacgcgatc
     2221 ggcaacatcc ttcacatcgg cgacgcgctc gcgaagatag acgtcgtcga agctgcggaa
     2281 gcgcgcctgc agttcttcct gcacctcggc cagcgcccac tccgcgttga cctgctcggc
     2341 ctcgatcttc tgcacgacct tgtccgcgaa tgatggatcg ctgaccatca tcgcgtgcgc
     2401 ctcgaagatc gacgcgtatt cgtcgcccat cgagcgcttg accttctgtt tgaggtcgag
     2461 aagctggctc tgcgtcttct cgatcgcttc gagaaagcgg ttgacctcgg acggcacttc
     2521 gtcatcgcgg atcggcacgc gatacaccgc tgcctcgagc cgttcgacaa tcaccgcgcg
     2581 tccgatggcg atgccgggag agacgccgat ccctttgtag gttcggatgc tcgtgcggct
     2641 gggtgaggtc gtggtgggca t
//
LOCUS       DATLVW010000112         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_541396, whole genome shotgun sequence.
ACCESSION   DATLVW010000112 DATLVW010000000
VERSION     DATLVW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541396"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..514)
                     /locus_tag="VNN25_03800"
     CDS             complement(374..514)
                     /locus_tag="VNN25_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90681.1"
                     /translation="MEFVLGAMGREKQLKGWNRKRKIELVQRMNAKWIDLTAEWSDEP
                     TE"
     gene            842..1912
                     /locus_tag="VNN25_03805"
     CDS             842..1912
                     /locus_tag="VNN25_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXH90682.1"
                     /translation="MTSRIPFAVSLIVATLAMSASAQSGDAPFVVGSAKAARGQKATG
                     TIEVPAGVDAALSIAVVVAHGAHPGPVLALVAGAHGTEYSSIIALERVIERLDPATLS
                     GTVIVVPLVNPRSFEQKIAHVNPIDNKSMNRFYPGRMDGTQTERASYLLTKEVVERAD
                     HLIDLHGGDLDESLLPYSYWTVSGNAPQDAISRQMLLAFGVSPIIISADRPRDPAASR
                     YLENTATTRGKPSITVEAGHAGTTETDDVELLANGCFNVMRFLKMVPGEVRPIENPVW
                     VEAIQSVNSDQTGIFYPLVRRGTYVSQGMKIGYVTDYTNRTILDARAPAAGIVMFIRA
                     VPSMTKGETIANIGIVANQAPN"
     gene            complement(1936..>3001)
                     /locus_tag="VNN25_03810"
     CDS             complement(1936..>3001)
                     /locus_tag="VNN25_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXH90683.1"
                     /translation="GIVVVLILLHFRWSAGMRDRAEKMTRFKPLQTIIYWLQYLIVTT
                     ILGFPLAVYEGFFRERQYGLATQTFGPWMGDQGKELLLSAVLGAIIAVLLFGVVRKLA
                     GSWHIWASIVTMLFLIFVVMIAPVYIVPIFNNLKRLDNPKVTVPIISMARANSIPVND
                     VYEMDASRQTTRMSANVSGFGKTMRITLNDNLLRRGSLEEIQAVMGHEMGHYVLNHIP
                     KDIEFFLIVIVAGFAFLRWALDWSLGKWGERWGIRGIGDTAVLPLVVLIFSIFFFILT
                     PILNTHIRTAEREADMYGLNASRQPDGFAQAAIHLGEYRKMSPGPVEEWIFFDHPSGR
                     NRILAAMKWKAENLKLFEGH"
BASE COUNT          584 a          934 c          905 g          578 t
ORIGIN      
        1 caagttcgta gcgccttggt gtcgatagat taaatagtcc aatcgagatt ggtgtcatcc
       61 tgagactgcc ggagccgccc gcgcgaatcg cgatgtttgc acggttcgtg ctcggcggcg
      121 taggacgtcg aaggatctca aattgcgcag atcgcgttcg tccagcgcga gcgatcgtgt
      181 tcaaagacgc tcgaacgaga tcgtcgcatt ttgagatcct tcgaccgtcc tccgccgccg
      241 atgcctgccg gggcacgagc agcgaatctt gcggcggctt cggcggctca ggatgacacg
      301 gcgggcgttt acccttcctg cgaggcttac cgaatcgctg ctcatcagca tcggtgtcca
      361 gggacgacac ggatcactcc gtgggctcgt cagaccactc cgcggtgaga tcgatccact
      421 tggcattcat ccgctgcaca agctcgatct tgcgcttccg gttccaaccc ttcagttgtt
      481 tctcccgtcc catggcgccg agtacgaact ccatcgcgct cgccccaacc gtgtcatcct
      541 gagccgccgg agccgcgcct tacgctgcac gaacccccgg caggtattgc ggcggaggac
      601 ggtcgaagga tctcaaaata cgaagatctt gttcgagcgg tctttgatca cgatcgctcg
      661 cgctggacga acacgattcg cgaagtttga gatccttcga ccgtcctgcg ccgccgatgt
      721 ctgccggggc acgagcagcg aatcttgcgg cggcttcggc ggctcaggat gacacatcac
      781 actttgcgcg tatcgtcgta tcgcttccat cggctgtatc gtacccgtac gaggtgaccc
      841 gatgacttcg agaatcccct tcgcggtttc cctgatcgtt gcaaccctgg ccatgtccgc
      901 gagcgcgcaa tccggcgacg ctcccttcgt cgtcggcagc gcaaaggcgg cgcgcggaca
      961 gaaggctacc ggtacgatcg aggtgccggc cggcgtcgat gcggcgctga gcattgcggt
     1021 cgtggttgcg catggcgcgc atccgggacc agtgcttgcg ctcgtggcgg gagcacacgg
     1081 aaccgaatac tcgtcgatca tcgccctgga gcgcgtcatc gaacggctcg atcctgccac
     1141 gttgtcgggc accgtgatcg tcgttccgct ggtcaacccg cgttcgttcg aacagaagat
     1201 cgcccatgtg aacccgatcg acaacaagag catgaaccgc ttctatcccg gccggatgga
     1261 cggcacccag accgagcgtg cgtcgtatct cctgacaaag gaagttgtcg agcgcgccga
     1321 tcatctgatc gaccttcatg gaggcgatct cgacgaaagc ctgctgccgt acagttactg
     1381 gacggtcagc gggaacgcgc cgcaggacgc catctcgcgt cagatgctgc tggcgtttgg
     1441 cgtttctccc atcatcatct ccgcagatcg cccgagagat cctgccgcgt cgcgctacct
     1501 cgagaacacc gccaccacgc gcggcaagcc atcaatcact gtcgaagcgg gtcatgcggg
     1561 gacgacggaa accgacgacg tcgagttgct cgcgaacggc tgtttcaacg tcatgcggtt
     1621 cctgaagatg gttcccggcg aggttaggcc gatcgagaac ccggtatggg tcgaagcgat
     1681 ccagagcgtg aacagcgacc agaccggcat cttctatccg ctcgtacggc gcgggaccta
     1741 tgtcagccag gggatgaaga tcggttacgt caccgactac acgaaccgca cgatcctcga
     1801 cgcgcgcgcc ccggccgcgg ggatcgtcat gttcattcgc gccgttccgt cgatgacgaa
     1861 aggcgagacc atcgccaata tcgggatcgt ggcgaaccag gcgccgaact agtccggcgc
     1921 ctgctggttg cgccgctagt gaccctcgaa cagcttcaaa ttctccgcct tccatttcat
     1981 cgccgcgagg atgcgattcc gcccgcttgg atggtcgaag aagatccact cttcgaccgg
     2041 gcccggcgac atcttgcggt actcgccgag gtggatggcg gcttgcgcga agccatcggg
     2101 ctggcggctg gcgttcaggc cgtacatgtc cgcctcccgc tccgcggtgc ggatgtgcgt
     2161 attgaggatt ggcgtcagaa tgaagaagaa gatcgagaag atcaggacga cgagcggaag
     2221 cacggccgtg tcgccgatcc cgcgaatccc ccatctctct ccccatttcc cgagcgacca
     2281 atccagcgcc cagcgcagga aggcgaaacc ggccacgatc acgatcagga agaactcgat
     2341 gtccttcggg atgtgattga gaacgtagtg ccccatctcg tgacccatca cggcctggat
     2401 ctcttccaat gatccgcggc ggagcaggtt gtcgttcaac gtgatgcgca tcgtcttccc
     2461 gaagccgctc acgttggcgc tcatgcgcgt ggtctgccgc gaggcatcca tttcgtagac
     2521 atcgttcacc gggatgctgt tcgcgcgggc catgctgatg atcggcacgg tgaccttcgg
     2581 gttgtcgagc cgcttgaggt tgttgaagat cggaacgatg tagaccggcg cgatcataac
     2641 gacgaagatc aggaagagca tcgtgacgat ggatgcccag atgtgccagc ttccggcaag
     2701 ctttcgcacc acgccgaaaa gaagaacggc gatgatggct ccgagcaccg cgctcaggag
     2761 aagctctttc ccctgatcgc ccatccacgg accgaacgtc tgcgtggcaa gcccgtactg
     2821 acgctcgcgg aagaaccctt cgtagaccgc aagcggaaac ccgaggatgg ttgtcacgat
     2881 cagatattga agccagtaga tgatcgtctg cagcggttta aagcgggtca tcttctccgc
     2941 ccggtcgcgc atccccgcgg accagcggaa gtggagaagg atgagcacga cgacgatgcc
     3001 g
//
LOCUS       DATLVW010000113         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_130499, whole genome shotgun sequence.
ACCESSION   DATLVW010000113 DATLVW010000000
VERSION     DATLVW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130499"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1641
                     /locus_tag="VNN25_03815"
     CDS             304..1641
                     /locus_tag="VNN25_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH90684.1"
                     /translation="MPERDPLVAPPFDQGVFLLHYNRGREAFQEARYAEARRELENAQ
                     RMRPDDADVLNLLGLVYFKTNAFPEAEVIYRRLADENPNVFILHSNLGLILFKQGKLD
                     ESETFLLRAIELRPNYAKSHLYLGLLYRQRRRYPLALEHLRFAGADKIVREVEAEIRP
                     QSPAPLPMPVVPTPPPLRRETQRISTQKITPITDARTSPGRTMMTQPGKVPMPAASTE
                     PGVLDTAHTAARKLQDAVDETQLADDKRLDLAHKIEGASKTFTLHENGFLEINFGRSV
                     HVKRGTVSSYSGNLKFVAESGLLGTTAQTLVKAVGQGKIFVYEKGRKTFLLDLNEEFI
                     YVEGSNLLALEDTLTYRVEPIYDGTYQRKIDTVKIFGKGSLAISTHIEPLTLRVTREY
                     PLSISSNALVAWTGNVIPTVMDDKSLENVMIENVDEAGFKIRFEGDGVVVSEQ"
     gene            1694..1972
                     /locus_tag="VNN25_03820"
     CDS             1694..1972
                     /locus_tag="VNN25_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90685.1"
                     /translation="MRKRDQAPPVPPGETWSKITVVLLDRHVAYLDRIAVDIRLQRGV
                     AISRAELIRGLIEAASKTGVTLSDAPDNHGMISLLRGAWARGGSRKRR"
     gene            complement(1969..>2266)
                     /locus_tag="VNN25_03825"
     CDS             complement(1969..>2266)
                     /locus_tag="VNN25_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXH90686.1"
                     /translation="QDINAAVGSESGIPDEKLLALPEWESSPLFDSIERLVLEYADAM
                     TITGRDVSEELFARVREQFDDDAIVELTEIVAWENASSKFNRALRIPSQKLWNR"
BASE COUNT          455 a          710 c          664 g          437 t
ORIGIN      
        1 ggggagtcgc tgagacgggt gtcggtattc gggggtcggg tgtcggaggg gaatccaata
       61 ccttgaaagc tcccgcggtc agcctaacgc cggcctccgg gtagtgggtc aacttgaaaa
      121 gccgatcacc actgaggcac agaggtcaca gaggtcacag agattgcctc tgtgttctct
      181 gtgtctctgt ggttgccttt gcgtcggcga ccacgatcag ccgtccaagt tgacccatta
      241 cggacctccg acacccgacc cccgacaggc gacacccgca ccactgatac aatcgcccac
      301 cgaatgcccg aacgtgatcc ccttgtcgct ccgccgttcg accagggcgt gtttctgctt
      361 cactacaacc gcggacgcga agcgtttcag gaagctcggt acgccgaggc gcggcgcgag
      421 ctcgagaatg cgcagcggat gcggccggat gacgccgacg tcctgaacct cctcgggctc
      481 gtctacttca agacgaacgc gtttcctgag gccgaggtga tttaccggcg gttggcggat
      541 gagaatccca atgtcttcat tcttcactcc aacctcggcc tgatcctctt caaacagggc
      601 aagctcgatg agtcggagac gttcctcctt cgtgcgatcg agcttcgtcc gaattacgcg
      661 aagtcgcacc tctacctcgg ccttttgtat cgtcagcgtc gtcggtaccc tctggcgctg
      721 gagcatcttc gcttcgccgg cgccgacaag atcgtgcgcg aggtcgaggc cgagatccgg
      781 ccgcagagcc cagcacctct tccgatgccg gtggtgccga ctccgccgcc gctacgccgc
      841 gagacgcagc gaatcagcac gcagaagatc actccgatca ccgacgcccg cacctctccg
      901 ggcagaacga tgatgacgca gcccggaaag gtgccgatgc ctgccgcctc gacggagccc
      961 ggcgtactcg acacggcgca cacggctgcg cgcaaactgc aggacgcggt cgatgagacg
     1021 cagctggccg acgacaagcg gctcgacctc gcgcacaaga tcgaaggagc atcgaagacc
     1081 ttcacgttgc acgagaacgg cttcctcgag atcaacttcg gacgcagcgt ccacgtcaag
     1141 cgcggcacgg tctcgagcta ttcaggcaat ctcaagttcg tcgccgaatc cggtctcctc
     1201 ggcacgaccg cgcagacgct tgtgaaagcg gtgggacaag gaaagatttt cgtctacgag
     1261 aagggccgca agacgtttct gctcgatctc aatgaagagt tcatctacgt cgaggggagc
     1321 aaccttctgg cgcttgaaga cacgctgaca tatcgcgtcg agccgatcta cgacggcacg
     1381 taccagcgca agatcgacac agtgaaaatc ttcggcaaag gctcgttggc gatctcgacg
     1441 cacatcgagc cgctgacgct tcgtgtgacg cgcgaatatc cattgtcgat ttcttcgaat
     1501 gcactcgtcg catggaccgg caatgtgatt ccgactgtca tggacgacaa gtcgctcgag
     1561 aacgtgatga tcgagaacgt cgacgaggct gggttcaaga tccgcttcga aggcgatggc
     1621 gtggtcgtca gcgaacagta gcttttccgc gtcgcaccgt acttatgccc ggatgggaag
     1681 gtacaatccc gccatgcgca aacgcgacca ggctccccct gtaccacccg gtgaaacgtg
     1741 gtcgaagatc actgttgtat tactcgatcg tcatgtggcg tatctcgatc gcattgcggt
     1801 cgacatccgg ctgcagcgcg gcgtggccat ttcgcgagcc gagctgattc gcggactgat
     1861 cgaggccgcg tcgaaaaccg gcgtcaccct ttccgatgcg cctgacaatc acggaatgat
     1921 cagtctgctc cgcggcgcgt gggcccgagg cggctcgcgt aagcggcgtt aacgattcca
     1981 cagtttctgc gatgggatcc gtagcgcgcg gttgaatttc gacgacgcgt tttcccacgc
     2041 tacgatctcc gtcagctcga cgatcgcgtc gtcgtcgaac tgctcacgca cccgcgcgaa
     2101 caactcctcc gatacatcgc ggccggtgat tgtcatggca tcggcgtact cgaggacaag
     2161 gcgctcgatg ctgtcgaaca atggagacga ttcccattcc ggcaacgcca ggagcttctc
     2221 atccgggatg cctgactcgc tgccgaccgc agcgttgatg tcctgt
//
LOCUS       DATLVW010000114         5221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_337667, whole genome shotgun sequence.
ACCESSION   DATLVW010000114 DATLVW010000000
VERSION     DATLVW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337667"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VNN25_03830"
     CDS             <1..886
                     /locus_tag="VNN25_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXH90687.1"
                     /translation="SEGKNDTYWFAPIVADAEAGFGGCLNAFELMKQMIECGAAAVHF
                     EDQLASEKKCGHLGGKVLLPTSQFIRTLSAARLAADVMGVPTLVVARTDANSATLLTS
                     DIDERDHPFLTGERTAEGFFMIKSGVDAAIARAIAYAPYADLLWMETSTPDIGEARRF
                     ADAVHAKYPGKMLAYNCSPSFNWAKKLSADEIASFQRELGELGYKFQFVTLAGFHALN
                     FSMFELARQYREEGMTAYSRMQQSEFAAEAHGYTATKHQHEVGTGYFDEVMNVISNGQ
                     SSTTALAGSTEAAQFGRH"
     gene            complement(1090..1446)
                     /locus_tag="VNN25_03835"
     CDS             complement(1090..1446)
                     /locus_tag="VNN25_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXH90688.1"
                     /translation="MSINSAEFERLVEEALDSLPERFADLLENIVVVVEEEPDASDLD
                     VMDDPNGELLGIYRGTPLTRRSFSAMTMPEQVAIFRGPILRVTRSAQEAKRQIRETVI
                     HEIGHHFGLSDDDMSY"
     gene            complement(1450..4518)
                     /locus_tag="VNN25_03840"
     CDS             complement(1450..4518)
                     /locus_tag="VNN25_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXH90689.1"
                     /translation="MRKLAVAFLFLLLVSTTLNAQYFGRNKVQWEQFDFKVLKTEHFD
                     IYYYDREADVVNDVGRQAERWYTRLSRTFNHTFNRKPIVLYANSADFQQTTTTPELIG
                     EGTGGFTDPFMNRVVLPLTGDYAENDHVLGHELVHVFQFDIAASISNSRRRFNLEMMP
                     LWIVEGMAEYFSKGRVDPLTSMWIRDAEQHNRLPNLRQLQGDPYYFPYRYGQALLAYI
                     GGRFGDDAVVRYFLSAGMVNPEAAFDRALGVPAKQIFLDWQESAHEIYAPIVADRPAE
                     PGTPLIGSKKGVRGNLNVGPAYSPDGKYIAFLSTRAIFDIDLFLADAQTGRVLRRLAS
                     TDRNGHFEALRFVDSAGSWSADSRKLAFITFQHGDNYLGIVDVDSGRVDTVKVPGIDA
                     ITSVAWSPDGHTMVIAGQATGVSDLFLFDTESKEVHRLTNDKYADLQPAFSPDGRTIA
                     FVSDRGAGPEANLEQLYFQGMRISTIEISSGTIKTLPLFTNAKNINPQYGPDGSIYFI
                     ANPEGIADVYRYSTDGRINRVTRVQTGVSGITDMSPALSVAQRTGDITFSLYENDNYN
                     IYRLNANPPTIAVETAATPASIARAAQLPPLRGTGSTITAYMQNPAEGLLPASTVFQQ
                     TSYKPSLHIAYLGPPTVGVGVSSFGTGVGGSVSAYYSDVLGYHNIGVTFQGGGTSGVG
                     TIADQLAGEVFYLNQRHRLNWGIDTTHLPYVTAFTSSGQAPVTINGQTFLADVIQQER
                     DIQRYDDVSGIVQYPFSQTRRLEFSGGFEHQALKSEVETVFIVNNQIVSDTTDQSPFN
                     FSLSLAHASLAFVGDSSSFGFLSPVRGTRYRYEAAAYSGDLKFQTALADWRKYYFVNP
                     VTFAVRGIYYGRYGADAENGRLTPLYIGQDTLIHGYDINSIGLDECVSAPGSTSCPVF
                     DRLVGSRVAVGNAEVRVPLVGNKQFGLMSGFVPTEFFAFADAGAAWSKNDSVKFRFEK
                     NNATDHVPVTSVGAGLRLLLAYIPLEFYAAKPFQRPTRNWVTGFNIVAGW"
     gene            complement(4670..>5221)
                     /locus_tag="VNN25_03845"
     CDS             complement(4670..>5221)
                     /locus_tag="VNN25_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXH90690.1"
                     /translation="GSRSPEKLRDSVAGEGERVSSGTFEEAASFGDLIVLATHGVATQ
                     EAINLADKKNFGHKVVIDATNPLDFSTGAPRLSVGHTDSLGEQIQLALPDARVVKAFN
                     TVGNGLFFKPDLPGGPPDMFIAGNDAEAKKLVSQVCEAFGWGVIDLGGIESSRLLEPM
                     CMVWVLHGVLSGSWTHAFKMLHK"
BASE COUNT         1014 a         1542 c         1685 g          980 t
ORIGIN      
        1 cagcgaaggg aagaacgata cctactggtt cgcgccgatc gtggccgatg ccgaggcagg
       61 tttcggcggc tgcctcaacg cgttcgagtt gatgaagcag atgatcgagt gcggcgccgc
      121 ggcggtccac ttcgaggacc agcttgcctc cgagaagaag tgcgggcacc tcggcgggaa
      181 ggttcttctg ccgacgtcgc aattcattcg cacgctttcc gcggcgaggc tcgcggctga
      241 cgtcatgggc gtcccgacgc tcgtcgtcgc gcgaaccgac gcgaacagcg ccacgctcct
      301 caccagcgac atcgacgagc gcgatcatcc gttcctcacc ggcgaacgca ccgccgaggg
      361 cttcttcatg atcaagagcg gtgtcgatgc cgcgatcgcg cgggcgattg cttacgcgcc
      421 gtacgccgac ttgttgtgga tggagacgtc gacgccggac atcggcgaag cgcggcgctt
      481 cgcggatgca gtccacgcga agtatccggg gaagatgctc gcctacaact gctcgccgtc
      541 gttcaactgg gcgaagaaac tatcggcaga cgagattgcc agttttcagc gcgagctggg
      601 cgagcttggc tacaagttcc agttcgtcac cctggccggc ttccacgcgc tgaacttctc
      661 gatgttcgag ctggcgcgcc agtatcgcga ggaagggatg accgcttact cgcgcatgca
      721 gcaatccgag ttcgccgcgg aagcgcacgg ctacacggcg acgaaacatc agcacgaggt
      781 tggcaccggc tatttcgacg aggtcatgaa cgtgatctcg aacgggcaat catcgacgac
      841 ggcgctggcc ggatccaccg aagccgcgca gttcggcaga cattgaggag acatcatgga
      901 agacatccgc gactaccgat tcgaagaaga gaacgacgtc accgaagaag agcgcgagta
      961 cgacgccgcg cagcgtgacg aagtgccgtt tcacatcccg cgggattgat tcttgaacca
     1021 caaagacaca gaggacacaa aggtttctct gtgtcctcag tgtctctgtg gttaaagcct
     1081 tttcaaactt caatacgaca tatcgtcatc gctcagcccg aagtggtggc cgatctcgtg
     1141 gatgaccgtt tcgcggatct ggcgctttgc ctcctgggcg ctgcgcgtca cgcgaagaat
     1201 cgggccgcga aagatggcga cctgctccgg catcgtcatt gcgctgaaac tccggcgcgt
     1261 cagcggcgtg ccgcgataga tgccaaggag ctcgccgttg ggatcgtcca ttacatcgag
     1321 gtccgaggca tcgggctcct cctcgacgac gaccacgatg ttctcgagca ggtccgcgaa
     1381 ccgctccggg agcgagtcga gcgcctcttc gacgaggcgc tcgaactccg cggaattgat
     1441 actcacccgc taccagccgg ccacgatatt gaaaccggtc acccagttgc gcgtcgggcg
     1501 ctggaacggt ttggctgcgt agaactcgag aggaatgtac gcgagcagga gccgcaaacc
     1561 ggcccccacg ctcgttaccg ggacgtgatc ggtggcgttg ttcttctcga aacggaactt
     1621 cacggaatcg ttcttgctcc aggccgcgcc ggcatcggcg aacgcaaaga actccgtcgg
     1681 aacgaagccg ctcatcagac cgaactgctt gttgccgacc aggggcacgc gaacctcggc
     1741 gttgcccaca gccactctcg aaccgaccag gcggtcgaac actgggcacg atgtcgagcc
     1801 cggcgcgctg acgcactcgt cgagaccgat cgagttgatg tcgtacccgt ggatcagcgt
     1861 gtcctgaccg atgtaaagcg gcgtaagtct tccgttctcg gcgtccgcgc cgtagcgtcc
     1921 gtaatagatc ccgcgaaccg cgaacgtgac cgggttcacg aagtagtact tgcgccaatc
     1981 cgcgagagcg gtctgaaact tcaggtcgcc cgagtacgcc gccgcttcgt agcgatagcg
     2041 cgttccgcgc acgggtgaga ggaatccaaa cgaagaacta tcgccgacga acgctagtga
     2101 ggcgtgagcg aggctcagcg agaaattgaa cggcgactgg tccgtcgtgt cggagacgat
     2161 ctggttgttg acgatgaaga cggtctcgac ctcggacttc agtgcctgat gttcgaaccc
     2221 gccgctgaat tcgaggcggc gcgtctggct gaatgggtac tggacgatgc ccgacacgtc
     2281 gtcgtagcgc tgaatgtcgc gctcctgctg gattacgtcg gcgaggaagg tctgcccatt
     2341 gatcgtcacc ggcgcctgtc ctgacgaggt gaacgcggtc acatacggca agtgcgtcgt
     2401 gtcgatgccc cagttcaagc gatggcgctg gttgaggtag aacacttcgc ctgcgagctg
     2461 gtcggcgatc gtgccgacgc ctgaagttcc gccaccctgg aaggtcacgc cgatgttgtg
     2521 atagccgaga acgtcgctgt agtaagcgct gacactgccg cccactccgg taccgaatga
     2581 gctgacgccg acgccaacgg tcggtggtcc gaggtacgca atgtgaagcg acggcttgta
     2641 tgaagtctgc tggaataccg tgctcgccgg caacagaccc tcggcaggat tctgcatgta
     2701 cgcggtgatc gtcgagccgg tgccgcgcag cggcggaagc tgggcggccc gcgcgatcga
     2761 tgcgggcgtg gcagccgtct ccaccgcgat ggtcggcggg ttggcgttca accggtagat
     2821 gttgtagttg tcgttctcat agagtgagaa ggtgatgtcg ccggtgcgct gcgcgaccga
     2881 cagggccggc gacatgtccg tgattcccga gacgccggtc tgcacgcgcg tgacgcgatt
     2941 gatgcgccca tcggtcgagt agcggtaaac gtcggcgatg ccttcgggat tggcgatgaa
     3001 gtagatgctg ccgtcgggtc cgtactgcgg attgatgttc ttcgcgttcg tgaagagcgg
     3061 gagcgtcttg atcgtaccgc tcgagatctc gatcgtcgaa atgcgcattc cctggaagta
     3121 gagctgctcg agattggcct cagggccggc gccgcggtcc gaaacgaacg cgatggtgcg
     3181 tccgtccggc gagaaggccg gctgcagatc ggcgtacttg tcgttcgtca gacgatgcac
     3241 ctctttcgat tcggtatcga agaggaagag gtcggagact ccggtggcct gccctgcgat
     3301 caccatcgtg tgtccgtccg gcgaccaggc gacgctggtg atggcatcga tgccgggcac
     3361 tttgacggtg tcaaccctgc ccgagtcgac gtcgacgatg ccgaggtagt tgtcgccgtg
     3421 ctggaaggtg atgaaagcca gcttgcgcga gtccgcggac caactgccgg cggaatcgac
     3481 gaagcgcagc gcctcgaagt ggccgttccg gtcggtcgac gccagacggc gaaggaccct
     3541 gcccgtctgc gcgtcagcga ggaagagatc gatatcgaaa atcgcgcgcg tggagaggaa
     3601 cgcgatgtac ttgccgtcag ggctgtacgc gggtccgacg ttgagattac cgcgcactcc
     3661 cttcttgctg ccgatcagcg gcgtgccggg ttctgccggc ctgtcagcca cgatcggagc
     3721 gtagatctcg tgcgccgatt cctgccagtc gaggaagatc tgtttggccg gcacaccgag
     3781 cgcgcgatcg aacgccgcct cgggattgac catgcctgcg gagaggaagt agcggacgac
     3841 ggcatcatcg ccgaaacgtc cgccgatgta cgcgagtagg gcctggccgt agcgataggg
     3901 gaagtagtag ggatcgccct gaagctggcg aagattgggc aaccggttgt gctgctcggc
     3961 gtcgcgaatc cacatcgacg tgagcggatc gacacggccc ttcgagaagt actcggccat
     4021 cccttccacg atccagagcg gcatcatctc cagattgaaa cgccggcggc tgttgctgat
     4081 gctcgcggcg atatcgaact gaaagacgtg aaccaattcg tggccgagga cgtgatcgtt
     4141 ctccgcgtag tcgccggtca gcgggagcac gacacggttc atgaagggat cggtaaatcc
     4201 gcccgtcccc tccccgatca gctccggcgt cgtcgtggtc tgctggaagt cagcggagtt
     4261 cgcgtagagg acgatcggct ttcggttgaa cgtgtggttg aacgtgcgcg agagccgcgt
     4321 gtaccaccgc tccgcctggc gtccgacatc gttcacgacg tccgcttccc ggtcgtagta
     4381 gtagatgtcg aagtgctccg tcttcagcac tttgaagtcg aactgctccc actggacttt
     4441 gttgcggccg aagtattgag cgttcagggt ggttgaaacc aacaggagaa agaggaaggc
     4501 gactgcgagt ttgcgcatcg aatgaacctc tccgttgaaa cgtatgaatc cgctgggcgc
     4561 cgggctgtgc aaatccattg ccccttcgcg gcgacgcaat gctactcctg cacgggtaac
     4621 aagctgttgc aaacaggtga acgggcgcgg gttatggcca cgtctgaggt cacttgtgta
     4681 gcattttgaa ggcgtgggtc cacgatcccg acagcacgcc gtgaagaacc cagaccatgc
     4741 acatcggttc gagaagccga gaggattcga tcccgccgag gtcgatcaca ccccaaccaa
     4801 aggcctcgca cacctgactg accagtttct tcgcctccgc atcgttgccg gcgatgaaca
     4861 tgtcgggagg accgccgggc agatcgggct tgaaaaagag cccgttgccg acggtgttga
     4921 acgctttgac cacccgggca tcgggcagcg caagctgaat ctgctcgccg agcgagtcgg
     4981 tgtgcccgac ggataaccgc ggagcgccgg tgctgaaatc gagcggattg gtggcgtcga
     5041 tgacgacctt gtgaccgaag ttctttttgt ccgcgaggtt gatggcctct tgggtggcca
     5101 cgccatgagt agccaggacg atcagatcgc cgaagctggc cgcctcttcg aacgtgccgc
     5161 tggagacgcg ctctccctcc ccggcgacgg aatcgcgcag cttctccggc gagcgcgatc
     5221 c
//
LOCUS       DATLVW010000115         5205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_541450, whole genome shotgun sequence.
ACCESSION   DATLVW010000115 DATLVW010000000
VERSION     DATLVW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541450"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="ddl"
                     /locus_tag="VNN25_03850"
     CDS             complement(<1..499)
                     /gene="ddl"
                     /locus_tag="VNN25_03850"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="D-alanine--D-alanine ligase; DdlA; DdlB;
                     cytoplasmic; catalyzes the formation of D-alanyl-D-alanine
                     from two D-alanines in peptidoglycan synthesis; there are
                     two forms of this enzyme in Escherichia coli; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXH90691.1"
                     /translation="MPIRTLLIFGGRSAEHEVSILSARSVAEAAPKPRIEIVPLCIAK
                     DGRFASPERSARILGGDERSDRGDDDFSFDSWVRGAAIDVAFPLIHGTGGEDGTLQGY
                     LEILGIPYVGSSVTASAIGMDKGHMKYAFAAAKLPMVDFVQVHENDWRNERERIMRAV
                     NNALRL"
     gene            complement(743..1510)
                     /locus_tag="VNN25_03855"
     CDS             complement(743..1510)
                     /locus_tag="VNN25_03855"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXH90692.1"
                     /translation="MKLDTRRIVERLTTIESLAVIPRVASTNLVARRIVNECIENELS
                     LPKAMIVAEEQFAGRGRNERKWSSPAGKGIYATTLLTRPASEMTLVPLETANIVASYL
                     REVFAIDARIKWPNDIMVGGRKIAGILIEARMNEERAYMLIGTGINVEPVSDDDRPNA
                     TAISEVSPRGFHGIGVATEAFIEHLDQRLGHPPAREAVLEEWRSFTIHQTGDRIQCVI
                     GERTVEGAWNGIDEHGRALLRSGSETIEVSAGDLIVL"
     gene            complement(1596..4229)
                     /locus_tag="VNN25_03860"
     CDS             complement(1596..4229)
                     /locus_tag="VNN25_03860"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXH90693.1"
                     /translation="MATTEELNKHYEPKEIEGRLYEFWEKGEFFMPLLDAERPKFSIV
                     IPPPNITGRLHIGHALVMTLQDIIVRWKRMSGFNTLWLPGTDHAGIATQMIVERELAK
                     EGIARLDLGREKFVEKVWEWKESYGHQIIDQLKRLGSSADWTRLRFTLDEGLAHAVRT
                     AFVKLYDDGLIYRGIAMVNWCPHCRTAISDVEVEYEERASKIYHIDYPVADSDRVLTI
                     ATTRPETMLGDTALAVHPDDERYKDVIGREAILPIANRRIPIVADPILVDPEFGTGVV
                     KVTPAHDKNDYEAGKRNNLEQLQVIDEAGKMTEAAGAEFAGLDRFEARKKVIGLLRDS
                     GSLRDVKDYTHSIGFHGKCETTIEPMISRQWFVKIEPLAKPAIDAVRNGTIQIIPQSW
                     EATYFNWMENIHDWTISRQLWWGHRIPAFHCANGHTTVAIEDPATCPTCGSAEITQET
                     DVLDTWFSSGLWPFSTMGWPDDTTDLKIFYPTDVLITGFDILFFWVARMIMFGLRFTG
                     KAPFSQVFLNGLVRDEHGAKMSKTKGNVIDPLDVIDEFGADAVRFTLAIFASGRDIPL
                     AKARMQGYAAFANKIWNASRFAMMHIDTTLSSAKPIDRDSLKTVERWILSRLNDTTRD
                     VNKALSAYRFDEASNAIYKFFWHEFCDWYIEMVKPVLLGRHGTPEDQQRAKRVLLEVL
                     DRSLRLLHPFMPFITDEIWQKLGGIEPSVMVAPYPVPEESLEDEEAERMVKAVQAMIT
                     TVRDARAQRGFTPKDRFKLYVRAEGRDAAFFDSYSYLLTDLGRLNEVVVNAEPPEGVH
                     HDVVEGFTIAIEFPEKVVTKEQQERTERELEKSRKELASLDAKLVNEQFMNNAPPAIV
                     QGARARQTELRARIEKLMQNQ"
     gene            4325..>5205
                     /gene="tsaD"
                     /locus_tag="VNN25_03865"
     CDS             4325..>5205
                     /gene="tsaD"
                     /locus_tag="VNN25_03865"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXH90694.1"
                     /translation="MILGIETSCDETSVALLDREGSVRVNLIASQIARHQPFGGVVPE
                     IASREHLQHLFPLITRALEDAKLAWSDVDRIAVTAGPGLIGALLVGVAGAQGLAYGLG
                     VPLVPVNHLQGHIFSPYLNREGPSTPLPERFLSLVISGGHSSIYDVRDGVVVMLNRTR
                     DDAIGETFDKVAKFIGLPYPGGPEIEKHGREGKIDRKRFHFAMPQFNEKSLDFSYSGI
                     KAGAIRLAREHGVSAAGDPATLADFCATFQDALIDQLFDRLDIVWPMFDAPRPTEVAI
                     AGGVAANGPLRERMAEWG"
BASE COUNT          907 a         1631 c         1631 g         1036 t
ORIGIN      
        1 gcaggcgcaa cgcgttattg actgcgcgca tgatccgctc acgctcgttg cgccagtcgt
       61 tttcgtggac ctgaacgaag tcgaccatcg gcagcttcgc ggcggcgaag gcatacttca
      121 tgtgcccctt gtccatcccg atcgcggagg ccgtcacact gctccccacg tacgggatgc
      181 cgagtatctc cagataaccc tgaagcgtgc cgtcctcgcc gccggtgccg tggatcagcg
      241 gaaaggcgac gtcgatggcc gcgccgcgca cccaggaatc gaacgagaaa tcgtcgtcgc
      301 cgcggtcgct gcgctcatcg ccgccgagga tgcgggcgct gcgctcgggg gacgcgaagc
      361 ggccgtcttt cgcaatgcaa agaggcacga tctcgatgcg cggcttcggc gccgcctcgg
      421 ctacggagcg cgccgagaga atcgaaactt cgtgctccgc gctgcggccg ccgaagatga
      481 ggagtgtacg gatgggcatg gggcggagtg tagtggatca gagacaggag agaagagatc
      541 agagtaatca gagatcagag atcgggatca ggcgcgaccg acacgacggt aactcccgat
      601 ctctggtctc ttgtctccag gtctctaatt ttcaatattg gagatccggc gtgcagatat
      661 cggagacaag agatcaggaa tcggggcgcg accatcggct cgtaaccccc tgatctctaa
      721 tctcttgtct ctgatctctg cttcacagca cgatcaaatc ccccgcgctg acctcaatcg
      781 tctcactgcc gcttcgaagc agcgcgcggc cgtgctcgtc gatgccattc catgcacctt
      841 cgacggtccg ctctccgatc acgcattgga tgcggtcgcc ggtttggtgg atggtgaaac
      901 tgcgccattc ctcgagcacc gcttcgcgcg cgggcggatg tcccagccgc tggtcgaggt
      961 gctcgatgaa cgcctcggtc gctaccccga tgccgtggaa accgcgcggc gatacctcgg
     1021 agatcgcggt cgcgttcggc cggtcatcat ccgataccgg ctcgacgttg atccccgttc
     1081 cgatgagcat gtaggcgcgc tcttcgttca tccgcgcctc gatcaggatg ccggcgatct
     1141 tcctgccgcc gaccatgatg tcgttgggcc acttgatgcg cgcgtcgatg gcgaagacct
     1201 cgcgcagata cgacgccacg atgttcgcgg tctccagcgg caccagcgtc atctcgctcg
     1261 ccggccgggt cagcagcgtg gtcgcgtaaa tccccttgcc cgccggcgac gaccacttgc
     1321 gctcgttccg tccgcgcccc gcgaactgct cctcggcgac gatcatggcc ttcggcaacg
     1381 acagctcgtt ctcgatgcac tcgttgacga tgcgccgcgc aaccagattc gtcgaagcca
     1441 cgcgcggaat caccgccagc gactcgatgg tcgtgaggcg ttcaacgatg cggcgggtgt
     1501 cgagtttcat tgtgtgaggg agtcgcttgt cgggggtcgg gagccaagat tttgtcacgt
     1561 cactccgact cccgaccccc gacaaccgac ccccgctatt gattctgcat cagcttctcg
     1621 atgcgcgcac gaagctcggt ctgacgcgcc cgtgcgcctt gcacgatcgc tggtggggcg
     1681 ttgttcatga actgctcatt gaccagcttc gcatcgagcg acgcgagctc tttgcggctc
     1741 ttctccagct cgcgctcggt ccgttcctgc tgttccttcg tgacgacttt ctccgggaac
     1801 tcgatggcga tggtgaagcc ttccacgacg tcgtgatgca cgccctcggg tggctcggcg
     1861 ttcacgacga cttcgttcag gcggcccaga tcggtgagca gatagctgta tgagtcgaag
     1921 aacgcggcat cgcgtccctc ggcacgcaca tagagcttga agcggtcctt cggcgtgaag
     1981 ccgcgctgcg cacgggcatc gcgaacggtg gtgatcatgg cttgaacagc cttcaccatc
     2041 cgctccgctt cctcatcctc gagcgactcc tcgggcaccg gatacggcgc gaccatgacc
     2101 gacggctcga tgccgccgag cttctgccag atctcatcgg tgatgaacgg catgaaggga
     2161 tggagcagcc ggagcgagcg gtcgagcact tcgagcagga cgcgtttggc tcgctgctga
     2221 tcttccggcg tgccatgcct gccgaggagg accggcttga ccatctcgat gtaccagtca
     2281 cagaactcgt gccagaagaa cttgtagatc gcgttcgacg cttcatcgaa tcgataggca
     2341 gaaagcgctt tgttcacgtc gcgcgtcgtg tcgttgagac gcgagaggat ccaacgctcc
     2401 accgtcttca gcgagtcgcg gtcgatcggt ttcgccgacg acagcgttgt gtcgatgtgc
     2461 atcatggcga agcgcgacgc gttccagatc ttgttcgcga acgcggcata accctgcatg
     2521 cgcgccttcg ccagcgggat gtcgcgcccg gaggcgaaga tcgccaacgt gaaacggacg
     2581 gcgtcggcgc cgaactcgtc gatcacgtcg agcggatcga tgacattgcc cttcgtcttc
     2641 gacatcttcg cgccgtgctc atcgcgcacc aggccgttga ggaacacctg gctgaacggc
     2701 gctttgccgg tgaagcgaag gccgaacatg atcatccgcg ccacccagaa gaagaggatg
     2761 tcgaagccgg tgatcaggac atcggtggga tagaaaatct tcaggtcggt cgtgtcgtcc
     2821 ggccagccca tcgtcgagaa gggccagagt cccgacgaga accaggtatc gagaacgtca
     2881 gtctcctgcg tgatctccgc ggagccgcag gtcggacagg tagccggatc ctcgatcgcc
     2941 accgtcgtgt ggccgttggc gcaatgaaac gccggaatgc gatggcccca ccagagctgg
     3001 cgagagatcg tccagtcgtg gatgttttcc atccagttga agtacgtcgc ttcccagctc
     3061 tgcgggatga tctgaatcgt gccgttgcgc acggcgtcga tggccggttt cgccagcggc
     3121 tcgatcttca cgaaccactg ccgcgagatc atcggctcga tggtcgtctc gcacttgccg
     3181 tggaagccga tcgagtgcgt gtagtccttc acgtcgcgca gcgaaccgga atctcgcagc
     3241 aggccgatca ctttcttgcg cgcttcgaag cgatcgagcc cggcgaactc cgcgccggca
     3301 gcttcggtca tcttccccgc ttcgtcgatc acctgaagct gctcgagatt gttgcgcttt
     3361 ccggcttcgt agtcgttctt gtcatgcgcc ggtgtgactt tgacgacgcc ggtaccgaac
     3421 tccggatcga cgaggatcgg gtcggcgaca atggggatac gtcggttcgc aatcggcagg
     3481 atcgcttcgc ggccgatcac gtctttgtag cgctcgtcgt ccggatgaac ggccagcgcc
     3541 gtgtcgccga gcatcgtctc gggacgcgtg gtggcgatgg tcagtacgcg atcggaatcg
     3601 gccaccggat aatcaatgtg gtagatcttc gaggctcgct cttcgtactc gacctcaacg
     3661 tccgagatcg ccgtgcggca gtgcgggcac caattcacca tggcgatgcc gcgatagatg
     3721 aggccgtcgt cgtagagctt cacgaacgcg gtacgcacgg cgtgggcgag gccctcatcg
     3781 agcgtgaaac ggaggcgcgt ccagtcggcc gaggagccga ggcgcttgag ctgatcgatg
     3841 atctggtggc cgtacgactc tttccactcc cacactttct cgacgaactt ctcgcgcccg
     3901 agatcgaggc gggcgatgcc ctctttggcg agctcgcgct ccacgatcat ctgcgtggcg
     3961 atgccggcgt ggtcggtgcc gggcagccag agcgtgttga agccgctcat ccgcttccag
     4021 cgaacgatga tgtcctgcag cgtcatgacg agcgcgtggc cgatgtgcag ccgcccggtg
     4081 atgttcggcg gggggatgac gatcgagaat ttcggccgtt ccgcatcgag cagcggcatg
     4141 aagaactcac ccttctccca gaattcgtag agccgccctt cgatctcttt cggctcgtaa
     4201 tgcttgttca gctcttcagt ggtcgccatc ggagcgcgga ttataaggtg cggtcgcgag
     4261 gttgcgggtt ctcgcgagtc tcgcaacaaa tgcgccgcgc ggccgcgaca ctccgctccg
     4321 caccatgatc ctcggtatcg aaacctcctg cgatgagacc tcggtcgcgc tactcgaccg
     4381 cgaaggcagc gtgcgtgtga acctgatcgc ttcgcagatt gcccggcatc agccgtttgg
     4441 tggggtcgtg ccggagatcg cctcgcgcga gcacctacag catctctttc cgctcatcac
     4501 gcgagcgctt gaggatgcga agctggcttg gagcgacgtc gatcgcatcg ccgtcactgc
     4561 aggtccgggg ctcattggcg cgttgctcgt cggtgtcgcc ggcgcccagg gactcgcata
     4621 tggactgggc gtgccgctcg tcccggtcaa tcacctgcag ggtcacatct tctcgccgta
     4681 tctgaatcgc gaaggaccgt cgacgccgct tccggagcgt tttctgagcc tggtcatctc
     4741 cggcggccat tcgtcgatct acgacgtgcg cgatggcgtc gtggtgatgc tcaaccgcac
     4801 gcgcgacgac gccatcggcg agacgttcga caaggtggcg aagttcatcg gcttgcccta
     4861 tcccggcgga ccggagatcg agaagcatgg ccgcgaaggg aagatcgacc ggaagcgctt
     4921 tcacttcgcg atgccgcagt tcaacgagaa atcgctcgac ttttcgtact ccggcatcaa
     4981 ggcgggagcg attcgcctcg cgcgcgagca cggcgtgagc gcggcgggtg atccggccac
     5041 tcttgccgat ttctgcgcca cgtttcagga tgcactcatc gatcaactct tcgaccggct
     5101 cgacatagtc tggcccatgt tcgacgctcc gaggccaacc gaagtggcga tcgcgggggg
     5161 cgtggccgca aatggacctc tgcgcgagcg gatggcggag tgggg
//
LOCUS       DATLVW010000116         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_582797, whole genome shotgun sequence.
ACCESSION   DATLVW010000116 DATLVW010000000
VERSION     DATLVW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582797"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1683
                     /locus_tag="VNN25_03870"
     CDS             157..1683
                     /locus_tag="VNN25_03870"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXH90695.1"
                     /translation="MTFDEKLDELHRRKKESEAGGGEKRIERQHAEGKKTARERIALL
                     FDAGSFQELDQLVVHRSTDFGMEAQRVAGDGVVTGYGTIGGRLVYAFAQDFTVFGGSL
                     SETHAAKIIKIMDLAMKNGAPIVGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVVP
                     QISAIMGPCAGGAVYSPAITDFILMVKKTSYMFVTGPDVIRTVTHEEVTKETLGGAET
                     HNQISGVAHFAAESDEDCLATIRELLSFVPSNNMEDPPHAATTDDPNRVDDQLNDFIP
                     DSPNQPYEMRDLIRTIADEDHFLEVHQHFARNLVVGFIRLNGHSVGVVANQPAFLAGV
                     LDINASRKGARFVRFCDAFNVPLLVLEDVPGFLPGTDQEYSGIIIHGAKLLFALAEAT
                     VPKITVITRKAYGGAYCVMNSKHIRADMNYAYPTAEIAVMGAEGAVNILYKGAKDRES
                     KIAEYKDKFANPFIAAERGYVDEIIEPRFTRRKLITAFAMLRNKRDKNPPKKHGNIPL
                     "
     gene            complement(1710..2267)
                     /locus_tag="VNN25_03875"
     CDS             complement(1710..2267)
                     /locus_tag="VNN25_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90696.1"
                     /translation="MRWQKTALNLLYSATAVAFVVLVISNWRLRRSYDALQAQFSAFA
                     SRAIVPFHAGDVMPKLEVVDRAGHPVTIRTADWKRDSYVALVLPGCGPCSEQISAAEN
                     AKLPNVVLICLVSREAAAKKLENVSAGMTLYFVRPSTAASLHHRLDGFPRILRVSAEG
                     KITATCRSIVGCGGPSCTACDLPAK"
     gene            2945..>3194
                     /locus_tag="VNN25_03880"
     CDS             2945..>3194
                     /locus_tag="VNN25_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90697.1"
                     /translation="MRRLSHVVVSFALLAVPSIFAQNVGPLPPLTAHIDVKIINVDVT
                     VTDHAGRPVTGLTKDDFEIFEDGKLQTVKNFDIIENAVL"
BASE COUNT          682 a          946 c          942 g          624 t
ORIGIN      
        1 ggagtgggag agagcttccg ctccgacaca ctctgcgcga gcgtgcttgg caactcacat
       61 gtcgcccttc gaagaactaa cgactcactt gtcgcgcgca ggcaatcgag tcagcgcgtc
      121 tcccctccca ccccccactc cccacacccc acacccatga ctttcgacga aaaactagac
      181 gaactgcacc gccgcaagaa agagagcgag gcgggaggcg gcgagaagcg catcgagcgt
      241 cagcatgccg aggggaagaa gacggcgcgc gagcgtattg cgctgctctt cgatgccggc
      301 tcattccagg agctcgatca actcgtcgtt catcgatcga ccgacttcgg gatggaagcg
      361 cagcgcgtcg ccggcgacgg cgtcgtcacg ggctatggca cgatcggcgg ccgtctcgtc
      421 tacgccttcg cgcaggactt cacggtcttt ggtggatcgc tctctgagac gcatgccgcc
      481 aagatcatca agatcatgga tctggccatg aagaacggcg caccgatcgt cggcctcaac
      541 gactccggcg gcgcgcgaat ccaggagggc gtggtctcgt tgggaggcta cgccgacatc
      601 tttctgcgca acacgctggc ctcgggtgtc gtgccgcaga tctccgcgat catggggccc
      661 tgcgctggtg gcgcggtcta ttcgcccgcg atcaccgact tcattttgat ggtgaagaag
      721 accagctaca tgttcgtgac cgggccggac gtgatccgaa cggtgacgca cgaagaggtc
      781 accaaggaaa cgttgggcgg cgcggagacg cacaatcaga tctcaggtgt ggcgcatttt
      841 gctgccgagt cggacgagga ttgtctggct acgatccgcg agcttttgtc gttcgtgccg
      901 tcgaacaaca tggaggatcc gccgcatgca gcgacgacgg atgatcccaa tcgtgtcgac
      961 gatcaactca acgacttcat ccccgattcg ccgaaccagc cgtatgagat gcgtgatctg
     1021 atccgcacca tcgccgacga ggaccatttt ctggaggttc atcagcattt cgcgcggaac
     1081 ctggtcgtcg gcttcatccg gttgaatggc cattcggttg gtgtcgtcgc gaaccaaccg
     1141 gccttcctgg cgggagtgct cgacatcaac gcctctcgca aaggggcacg attcgtccgg
     1201 ttctgtgacg cgttcaacgt ccccttgctg gttctcgagg atgtgcccgg atttctgccg
     1261 ggcaccgatc aggagtacag cggcatcatc attcatgggg cgaaactgct gttcgcgctc
     1321 gccgaggcga ccgttccgaa gatcacggtc atcacgcgca aagcgtacgg tggcgcctac
     1381 tgcgtgatga actcgaagca catccgcgcg gacatgaact acgcctaccc gacggcggag
     1441 attgccgtga tgggcgcgga gggggcggtc aacattctgt acaagggcgc caaagaccgc
     1501 gagtcgaaga tcgccgagta caaggataag ttcgccaatc ccttcatcgc cgcggagcgc
     1561 ggatacgtcg acgagattat cgagccgcgc ttcacgcgcc ggaagctgat caccgccttc
     1621 gccatgctgc gcaacaagcg cgacaagaac ccgccaaaga agcacgggaa cattccgttg
     1681 taggaaacct ggtcgcgcgc gaggccgggc tattttgccg gtagatcgca ggcagtacag
     1741 gaaggtccgc cgcaaccaac gatcgaccgg caagtggccg tgatttttcc ttcagcagag
     1801 acacggagga tgcgagggaa gccatcgagg cgatggtgca ggcttgcggc ggtcgacggt
     1861 ctcacgaaat acagagtcat gcctgcagag acgttctcta actttttcgc tgcagcttcg
     1921 cggctcacga ggcatatgag tacgacgttg ggcagcttcg cattttccgc cgcgctgatc
     1981 tgctcgctgc agggtccgca acccgggaga accagagcaa cgtaggagtc acgcttccaa
     2041 tccgccgtcc ggatcgtcac ggggtggccc gcgcgatcga ctacttcaag cttcggcatg
     2101 acgtcgccgg catggaatgg gacgatcgct cttgaagcga aggcagaaaa ctgcgcttgc
     2161 agcgcgtcat acgagcgacg caagcgccag ttggaaatca cgagcacgac aaaagcgacc
     2221 gccgttgccg aatacagtaa gttcagggcg gtcttttgcc agcgcatagg gacacccact
     2281 cccacctctc aagggtgccg ccagtttacc agccagtgca gtagtaggtc gagcaagctc
     2341 cagggtcata ctggccatat tgatcgacgt taccgttgca gatgtttacc gagcaggcaa
     2401 tgttttcccc gttagcacag taccaggatt ccgtctcgaa aaatccgcta acgtctcccc
     2461 atctctccga tccgtcgcac ccgtgataat tctcgcctac aactgtctca aacgttgagt
     2521 ccgagtagta gatggtccaa gaacccgcga cgggcaacgc ttgcagggag gatccgacgg
     2581 caacaatgat cagtgccacc agtccacata ttttcagttt cgtcattgga tctttcctct
     2641 atttgtcacc gcgattttcg atctattccg gaagtgtgta tcgcccaaac tattctgcga
     2701 gagagagaat gtcaagatgg ccagtcatcg gactatcgat cggcgagtcc tcggaagaga
     2761 gtggcagacc ccacgtccgc aggaagcagg atgccgagac gtcaccattc gcatagcaag
     2821 gatatccggt cacagctgag ctcgagactc cagatctttg ttacacctac ggaacagctt
     2881 gccgtggtgc acggtgtgtt gtggcggact cggacgtaca atcggcgctt cagggggagg
     2941 atctatgcgc cggttgtcac acgtggttgt gtcgtttgcg ttgctcgctg tcccgtccat
     3001 cttcgcgcag aacgtcgggc ctctgccgcc gctgacggcg catatcgatg tgaagatcat
     3061 caacgttgac gtgacggtca cggaccacgc gggacgtccg gtcacgggcc tcacgaagga
     3121 tgacttcgag atcttcgaag acggaaaact gcagacggtc aagaacttcg acatcatcga
     3181 gaacgccgtt ctga
//
LOCUS       DATLVW010000117         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_171714, whole genome shotgun sequence.
ACCESSION   DATLVW010000117 DATLVW010000000
VERSION     DATLVW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171714"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VNN25_03885"
     CDS             <1..257
                     /locus_tag="VNN25_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90698.1"
                     /translation="PGDEVNIPKMEAILASISELIPYEVAEDEGDEPEDEEMASADER
                     GADAESAPPNDGAGFSHPIRRRRAEPVPLYSGKENPSWRL"
     gene            245..1756
                     /locus_tag="VNN25_03890"
     CDS             245..1756
                     /locus_tag="VNN25_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HXH90699.1"
                     /translation="MAAVTVEDLETRNGEIKARLKEIDSSFEGEALPEDERAEWNQLN
                     EELEGNDERIKELEARAARLVEIAGNGDSTARENGDGYSFMTRPHDVPRGDDIHDLTT
                     VRATSTSPLGQKSELRDRVLRFAERAHFPHSSIDSRKPGAPTREDVQAHIEGLLDRCV
                     ETVPGQTARYILEHGSPTYRRAFGKLLPSIAAGAPNIAGLTSEEQRALALSGTGGFPV
                     PVTLDPTIIPTSNSVVNPARAISRVVSITGSNTWNGVTSPAITAARVAEAGAATDNAQ
                     TLVQPTATVTKAHCFVPFSVEVAEDWTNLEAEFSNLLQDAKDDEEGAAFVTGVGTTVN
                     PQGFTVGATTTFAASTGLTVTAANVYGMENALPPRFRARSSIVANRGIYNVIRAIDTA
                     GGAALWLYISQGLVSQSPAPGNTGATLIGRPAWEASAMQATVVNASKIMVIGDFNYFL
                     ILDRIGMNIQSIPFLFGAGQGNLPTGQQGLYAWWRNTSKVLYANAFVAMTGTT"
     gene            1819..2001
                     /locus_tag="VNN25_03895"
     CDS             1819..2001
                     /locus_tag="VNN25_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90700.1"
                     /translation="MAPIQKTIKSPEAPRMPETVPVHIPPAKLPIPGPGMSSGGGGIT
                     PSPEPVDGSTKKVQKG"
     gene            2001..2150
                     /locus_tag="VNN25_03900"
     CDS             2001..2150
                     /locus_tag="VNN25_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90701.1"
                     /translation="MAQQPPNVHPKNPPTGKGKVDQTPGNASMGNSMHKPVTAYSDQQ
                     QEQRT"
     gene            2147..>2439
                     /locus_tag="VNN25_03905"
     CDS             2147..>2439
                     /locus_tag="VNN25_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90702.1"
                     /translation="MSAYAGYPHGQQQQKKSKQLSRTITGAATHGGLIQITSVGHTFR
                     DEDRVTITGVGGTVEANAANWRVAFIDADHFDLKGSTFTNAYTSGGTAKRTQG"
BASE COUNT          513 a          817 c          788 g          321 t
ORIGIN      
        1 agccaggcga cgaagtcaac attccgaaga tggaggcgat cctcgcgtcg atcagcgagt
       61 tgatccccta tgaggtcgca gaggacgaag gcgacgaacc ggaagacgaa gaaatggcat
      121 cggccgacga gcggggcgcc gatgccgaaa gcgcacctcc aaacgatggc gccgggttct
      181 cccaccccat ccgcagacgc cgcgcggaac cggtcccgct ctacagcgga aaggagaacc
      241 cctcatggcg gctgtgaccg tcgaggatct cgagacgcgc aacggagaga tcaaggcgcg
      301 gctcaaagag atcgattcct cattcgaggg tgaggctctg cccgaggacg aacgggccga
      361 gtggaaccag ctgaacgagg agctcgaggg aaacgacgag cggatcaagg agttggaagc
      421 acgcgcagcg cggctcgtgg agatcgccgg caacggtgac tcgacggctc gcgagaacgg
      481 cgacggctac agcttcatga cccgcccgca cgacgtcccc cgaggagacg acatccacga
      541 cctgacgaca gtcagggcaa ccagcacttc cccgctgggg cagaagagcg agcttcgtga
      601 tcgcgttctg cgctttgcgg agcgggcgca cttcccgcac tcatcgatcg actcgcgcaa
      661 gcccggtgcc ccgacacgcg aagacgtgca ggcgcacatc gaagggctgc tcgatcgctg
      721 cgttgagacg gtgccgggcc agacggcccg ctacatcctc gagcacggaa gcccgaccta
      781 caggcgggcc ttcggcaagc tcctgccctc gatcgcggca ggcgctccga acatcgccgg
      841 gctgaccagc gaggagcagc gcgcactcgc cctgagcggc acgggcggct tcccggtccc
      901 ggtaacgctc gatccgacca tcatcccgac ctcgaactcg gtcgtgaacc cggctcgcgc
      961 aatctcgcgg gtcgtgtcca tcaccggctc gaacacctgg aacggcgtca cgtctccggc
     1021 gatcaccgcc gcacgcgtgg cagaggccgg ggcggcaacc gacaacgcac agacgctcgt
     1081 ccagccgacc gcgaccgtga ccaaggcgca ctgcttcgtg cccttctcgg tcgaagtcgc
     1141 tgaggactgg acgaacctcg aggcagagtt ctcgaacctg cttcaggacg ccaaggacga
     1201 cgaagagggc gcagcgttcg tgaccggtgt cggcacgacc gtcaacccgc agggcttcac
     1261 agtcggagcg acgaccacgt tcgccgcctc gaccggcctg acggtgacgg cagccaacgt
     1321 ctacggcatg gagaacgcac tgcctccgcg cttcagggct cggagctcca tcgtcgccaa
     1381 ccgcggcatc tacaacgtca tccgcgcaat cgacacggcg ggcggcgctg ctctgtggct
     1441 ctacatctcg caggggctcg tctcgcagtc gccggctccg ggcaacaccg gggcgacgct
     1501 gatcgggcga cccgcctggg aggcatcggc catgcaggca acggtcgtca acgccagcaa
     1561 gatcatggtg atcggcgact tcaactactt cctgatcctc gaccggatcg ggatgaacat
     1621 ccagagcatt cccttcctgt tcggcgccgg ccagggcaac ctgccgaccg gccagcaggg
     1681 gctctacgcc tggtggagga acacctccaa ggtgctctac gccaacgcgt tcgtcgcaat
     1741 gacggggacc acgtaagtca gggagacagg ctggggggtc agggcggctg gccccccacg
     1801 ccaaaccgaa aggaagcgat ggcaccgatt cagaagacga tcaagagtcc agaggcaccg
     1861 cggatgccag aaacggtgcc ggttcacatt ccgccggcga agctgccgat tccgggtccg
     1921 gggatgagct cgggcggcgg cgggatcaca ccctcgccgg agccggtcga cggctcgacc
     1981 aagaaggtgc agaagggctg atggcgcagc agcctccgaa cgtgcatccg aagaacccgc
     2041 cgacaggcaa gggcaaggtc gaccagaccc ccggcaacgc ctcgatgggt aactcgatgc
     2101 acaagccggt gacggcctac agcgaccagc agcaggagca gcgcacatga gcgcctacgc
     2161 gggctatccg cacggtcagc agcagcagaa gaagtccaag cagctctcga ggacgatcac
     2221 aggcgcggcc actcacggag gattgatcca gatcacctcc gtgggccaca cctttcgcga
     2281 cgaggaccgc gtgacgatca caggagtcgg cggcacggtc gaggcgaacg cagccaactg
     2341 gcgcgtggcc ttcatcgacg ctgaccactt cgacctcaag ggttcgacct tcaccaacgc
     2401 ctatacgtcc ggtggcaccg ccaagcggac acagggata
//
LOCUS       DATLVW010000118         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_293728, whole genome shotgun sequence.
ACCESSION   DATLVW010000118 DATLVW010000000
VERSION     DATLVW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293728"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNN25_03910"
     CDS             <1..219
                     /locus_tag="VNN25_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HXH90703.1"
                     /translation="RPVVLPDTLDIEIGIVTPNQEIFLTLDGQEGLPIAQNDTVCINR
                     SPNPVTLIRTPKKSYFDVLRAKLKWGEG"
     gene            392..1261
                     /locus_tag="VNN25_03915"
     CDS             392..1261
                     /locus_tag="VNN25_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90704.1"
                     /translation="MNPTAKRALTTTRSIAVVGGIVGAGAHVGQYAALGFLDTKLNQV
                     LRAGKEFVPDGIHAVLGTLNRIAVSVYSHGYITLIMILCCGGLVWYLRRAPRVAPAVF
                     IGAFAVVVLLTLPAFTLFDLLHEPPDPARVPVTRFARAFLMPVVEGLRFSRVRPPGST
                     SWDPTAIRSALSTRYVIIVASTLYLFALAQMAMPDHKRMRRAANALVLVAVVGASYYW
                     GAIEEDTSYYRTVAKKGHAQHDEHAAFLIATVGNTCSIYSFHYGNEQLGEEFTDTDSE
                     RKDDILLTRLQQP"
     gene            1281..2159
                     /locus_tag="VNN25_03920"
     CDS             1281..2159
                     /locus_tag="VNN25_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90705.1"
                     /translation="MRSSLLLFTLLLVPQLSYSSGVRDLLNADTHNKEAIYSYGNKMD
                     FLVVGGPRDGVVTLRAFRGTRDQLIAQAHAWRPKCARLQHFVFGGWWVSSRILTFNSR
                     FDDWSLPEGTANGQWLFVFDVQPASQSVSIAGFQGIQELQLDQDTVDAYFKTGLEVTV
                     TNTKPTERTSKSNRTTPRTTPSEPATINMQILPGGSNATHGTNVIPAPVITSLSCSVK
                     QNVLRETTLVVSAGSHRQLPEVIAKRDAPLPVNASHARSRFAWFSWRRTREDRPSVSA
                     TGDESALMTSSTGNSP"
BASE COUNT          511 a          742 c          669 g          517 t
ORIGIN      
        1 cgccccgtcg tgctgcccga tacgctggac atcgagatcg gcatcgtcac cccgaaccag
       61 gagattttcc tcaccctcga cggccaggaa ggcctcccca tcgcccagaa cgacaccgtc
      121 tgcatcaaca gatcgccgaa ccccgtgacg ttgatccgca cgccgaagaa gagctacttc
      181 gatgtgttgc gggcgaagtt gaagtggggg gagggatagg gttttggtca atcgctcggc
      241 cggagaaatg acggccaggg gtagggaaaa gcgcgctcat gtccctccta agtagtaaac
      301 actcataacg aggaggactc cgccgggacg tagtccgttt cgacggctcg cggtttctca
      361 gggtccgttc cggcgattca gggagaatga aatgaacccg acagcaaagc gtgccctgac
      421 cactactcgc agcattgccg tcgttggtgg catcgtcggc gctggcgcgc acgtaggcca
      481 gtacgccgct ctcgggtttc tcgacacgaa gctcaaccag gtgttgcgtg ccggcaagga
      541 gttcgtccct gatggcattc atgcggtgct tggaacgcta aatcggatcg cagtcagcgt
      601 ctacagccac ggttacatca cccttatcat gatcctctgt tgcggcggcc tcgtctggta
      661 cttgcgccgc gctccacgag ttgccccggc cgtgttcatc ggtgcgtttg cggtggtagt
      721 tctcctcacc cttcccgcgt ttacgctctt cgatctgctc cacgagccgc ccgaccctgc
      781 ccgagttccc gtaacacgct ttgctcgagc ctttctgatg ccggtggtcg agggccttag
      841 gttctcgcgt gtcaggccac ccggaagcac gtcttgggat ccgaccgcta ttcgctcggc
      901 cctaagcact cgatacgtga tcatcgttgc aagtaccttg tatctctttg ctctagcgca
      961 gatggcgatg ccggatcaca aacgaatgcg ccgcgctgca aacgccctgg tactcgttgc
     1021 tgttgtcggc gccagctatt actggggagc gatcgaggaa gacaccagct actaccgcac
     1081 cgttgcgaag aaggggcacg cgcagcacga cgaacatgcg gcgtttctca tagcgaccgt
     1141 tggcaacacg tgttcgatat attcatttca ttatggcaat gaacagcttg gcgaagagtt
     1201 cactgatacg gatagcgagc ggaaggacga cattcttctg acgcgattgc agcaaccata
     1261 gttcaaaaaa cggaggaatt atgcgtagtt cattactcct gttcacctta ctcctcgtgc
     1321 cgcagctctc ctactcctct ggcgtgcgcg acttgctcaa cgcagacacg cacaacaaag
     1381 aggccatcta ttcatatggt aataaaatgg acttcctcgt cgttggcgga ccgcgcgacg
     1441 gggttgtaac gttgcgcgca tttcgaggca ctcgcgacca gctgatcgcg caggcccacg
     1501 cttggcgtcc caaatgcgcc cgtttacagc acttcgtctt tggaggttgg tgggtgtcat
     1561 cacgcatact gacgttcaac agtcgcttcg atgattggtc gcttccggag gggacagcca
     1621 acggtcaatg gctcttcgtg ttcgatgttc agcccgcctc acagagcgtg tctatcgccg
     1681 gctttcaagg gatacaggag ttgcagctcg accaggatac ggttgatgcc tacttcaaga
     1741 cagggctgga agttacggtt acgaacacca agccgaccga acgcacatcc aagtcgaaca
     1801 gaactactcc cagaactact ccgagcgaac ctgcgacgat caacatgcag attctgccgg
     1861 gaggaagcaa cgcgactcac ggtacaaacg tgatcccagc cccggttatc acctctctct
     1921 cgtgcagtgt taagcaaaac gtgctccgag aaacaacgct ggtcgtcagc gccggctcgc
     1981 acagacagct gccggaggtg atcgctaagc gggatgcgcc gttaccagta aacgcttccc
     2041 acgccaggag tcgctttgca tggttctcat ggcggcgcac cagggaagat cgcccctccg
     2101 tctccgccac cggtgacgag tcggcgttga tgacgtcttc caccggcaat tcgccgtgaa
     2161 cactcgtcag gaaactgctt cgtaattcga gcgaacgacc ccacgtctcg caactcgcgt
     2221 tcccgaggga tcctctcgta ctttctgcgc cgcctgattc gccatttggt aggccaagag
     2281 cgagatcgaa agcgtcggaa ggtacgaatc cgatacgatt cgcctcatga cgatcgagcg
     2341 acgcgccgct gtactactct tcctcgcttt caccgcctgc acgatgccga atacaaaacg
     2401 agaaacctct gtccctgaga ccatctacgc tggcggcac
//
LOCUS       DATLVW010000119         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_211151, whole genome shotgun sequence.
ACCESSION   DATLVW010000119 DATLVW010000000
VERSION     DATLVW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211151"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VNN25_03925"
     CDS             <1..1054
                     /locus_tag="VNN25_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HXH90706.1"
                     /translation="FDCADLVISPGFTHVVSSAHGAASDRKYDQRIGEARPIAELVAA
                     AAPAGFDLRLHQIAGIGDQVPLVRHVLDRAAEAVLVIYGDSLLSLDFAALLRFHEAVR
                     ERGAKVTIVSHRPHDLFRAESDDRTYHGILAIDGERRLTRFEEKPKRTEITELFHLAN
                     AAVFVCERPLFDEPQFRDAKNFSYDVFEPAVLLPQMPLYAFDISSGFRMDLGNTERFF
                     DANLKLLRGELLGPLPGQERSAGVRIGDGTIHDGASFTPPVLVGNGVRIERDAHIGPG
                     AVIADHCHIGARAAVRNAVLLEGCHVGAAAVVDTCVLGPHVRIGAGVTLPAHSVLGAF
                     SVAAGEDWPNWTEELR"
     gene            1051..>2866
                     /locus_tag="VNN25_03930"
     CDS             1051..>2866
                     /locus_tag="VNN25_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH90707.1"
                     /translation="MNAVSTVMFIDMVRSTALKYKDEDFHESVIRLYGVVQTVIPSNR
                     FLKFTGDGAMVAYSVDPDRPERAPLRAISNASKIIRNIDHLNYRFDTLFPIHVRVGIA
                     TGDTRQFKHGADDLIGKKVDLASRLCNEAEQDGMLVDDDTLTNAKLVAEDGTVIRELE
                     AGTRIALCERRLVLKGIPVMHGERFWDIAVEHFVDPPADDDSFSSGLVALYLRRKELK
                     GDFSPAKLLECAKKDSRFIVAGRTLLKWAEIEPSYLIEIAEARNLRLNFVIASDRALQ
                     YIDPDQLPTVKGDFKNALPKFRTLLEMDQMGSRQHVWLYESDLLILDGIVCAELSFEF
                     DDEDGKRSLFSRHAALQDINAVPWTVTAASPADRSEELANAKGAMLWVCTCPGGVLQQ
                     DRGCTAHGLHERTDVIRTKAKLLSPMGIAIEHQLRGWGEGLTSRNNHPRNYLPAMRPY
                     LECIAESRFTDVPPPVCVQMQVSSRCSTYCTMCDHFQEGRRRGKEELPLDDWQRIFAA
                     VAVCGAQTAIVSGGEPLVRDDIAILLRSVRRAGLRVGLLTNGVVDRQSEAEKEQVFEA
                     VATSADWVAVSVDGTESLDAKIRNLHVRDKSRTRAELLH"
BASE COUNT          535 a          882 c          901 g          548 t
ORIGIN      
        1 gttcgattgc gcagatctgg tcatctctcc gggctttacc cacgtcgttt cgtcggcgca
       61 cggtgcggcg tccgaccgca agtacgacca gcggattggt gaggcgcggc cgattgcgga
      121 attggtcgcg gctgcggcgc cagctggctt cgatttgcgc ctccaccaga tcgccggcat
      181 cggcgatcag gtgccgctcg tgcggcacgt cctcgaccgc gcggccgagg cggtgcttgt
      241 catctacggc gacagtctgc tctcgctcga cttcgccgca ctcctacgct ttcacgaagc
      301 tgtccgggag cgcggagcca aggtgacgat cgtgtcccac cgtccgcacg atctctttcg
      361 cgccgagagc gacgaccgga cataccacgg gatactggcg attgatggag agcggcgact
      421 gacccggttc gaagagaagc cgaagcgcac cgagatcacc gaactcttcc atctcgccaa
      481 tgcggcggtc ttcgtttgcg agcggccgct gttcgacgag ccacagtttc gcgacgcgaa
      541 gaatttctca tacgacgtct tcgagccggc ggttcttcta ccgcagatgc cgctctacgc
      601 cttcgacatc agcagcggtt tccgcatgga cctcggcaac acggagagat tcttcgacgc
      661 aaatctgaag ctgttgcgcg gcgagctgct tggccctttg ccgggacagg agcgaagcgc
      721 cggcgtacgc atcggcgacg ggaccattca tgacggcgcg agtttcactc cgccggttct
      781 cgtcggcaac ggcgtgcgca tcgagcgcga cgcccacatc ggtccgggtg ccgtgatcgc
      841 cgaccactgc catatcggcg cgcgcgccgc tgtacgaaac gccgttctcc tcgaaggctg
      901 ccacgtcggc gcggcggcgg tggtggatac gtgcgtgctc ggcccgcacg tgcggatcgg
      961 agccggcgtg acactgcccg cgcattccgt gctcggcgca tttagcgtcg ccgccggaga
     1021 ggattggccg aattggacgg aggagctccg atgaacgcag tttcaacggt gatgttcatc
     1081 gacatggtga ggtcgactgc gctgaagtac aaggacgagg actttcacga atccgtgatt
     1141 cgcctgtacg gcgtcgtcca gaccgtcatt ccctccaatc gcttcctgaa gttcacgggc
     1201 gacggcgcaa tggtcgccta cagcgtcgat ccggataggc cggagcgtgc gccgcttcga
     1261 gcgatcagca acgcgtccaa gattatccgc aacattgatc atctcaacta tcgcttcgat
     1321 actctgttcc cgatccacgt ccgcgtagga atcgccaccg gcgacacgag acagttcaag
     1381 cacggcgcgg acgatttgat cgggaagaag gtcgatctcg cgtcgcgtct ttgcaacgag
     1441 gcggaacagg acggcatgct ggtagacgat gacacgttga ccaatgcgaa gctcgtcgcg
     1501 gaggatggaa ccgtcattcg agagttggaa gcgggcactc gcatcgccct ctgcgaacgc
     1561 cggctcgtgc tgaaggggat tccggtcatg cacggcgagc gcttctggga catcgctgtg
     1621 gagcatttcg tcgatccgcc agccgacgac gactcgtttt cgagcggact ggtggcactg
     1681 tatctccgcc gcaaggaatt gaagggagac ttcagccccg ctaagttgct cgagtgcgcc
     1741 aagaaggact cccgcttcat tgtggccgga cgtacgctac tgaagtgggc cgaaattgaa
     1801 ccgagctatt tgatcgaaat cgcagaggcc cgtaatctca ggttgaattt cgtcatcgcc
     1861 tccgaccgcg cattgcagta catcgacccg gatcaactcc ccaccgtgaa aggtgatttc
     1921 aagaacgcgc tgccgaagtt tcgcaccctg cttgagatgg atcagatggg gtcgcggcag
     1981 cacgtgtggc tctatgaatc ggatctgctg atcctcgacg gcatagtctg cgcggagctg
     2041 tccttcgagt tcgatgacga agacggaaaa agatccttgt tctctcgcca cgccgccctg
     2101 caggacatta acgcggtgcc gtggacggtg acagctgctt cgccggccga tcgatcggag
     2161 gaactcgcca atgcgaaagg ggcgatgctg tgggtttgca cctgccccgg tggcgtccta
     2221 caacaagacc ggggttgcac cgctcacggc ttgcacgagc gtacggacgt cattcggacg
     2281 aaagcgaagc ttctatcgcc gatggggatc gcgatcgagc accagctccg cggttgggga
     2341 gaggggttga cgtcgcgaaa caaccatccg aggaattacc taccggccat gaggccctat
     2401 ctcgaatgca tcgccgagtc gcggttcacc gacgtgcctc ccccggtctg cgtgcagatg
     2461 caggtctcgt cgcgatgcag cacgtattgc acgatgtgcg atcacttcca ggaaggaaga
     2521 cggcgcggga aagaggagtt gccactcgat gactggcaac gcattttcgc cgccgtcgcc
     2581 gtctgcggcg cccagacagc gatcgtttcg ggcggcgagc cgctcgtgcg tgacgacatc
     2641 gccatactcc ttcgttccgt ccgtcgcgcc ggcctgcgcg ttgggctgct gacgaacggc
     2701 gtggtcgacc gtcagtccga ggcggagaaa gagcaagtct tcgaggccgt cgccacatcc
     2761 gccgactggg tggccgtctc cgtcgacgga accgagagcc tggacgctaa gatccgcaat
     2821 cttcacgtcc gggataagtc gcgcacgcgc gccgagctgc tgcacg
//
LOCUS       DATLVW010000120         9851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_376141, whole genome shotgun sequence.
ACCESSION   DATLVW010000120 DATLVW010000000
VERSION     DATLVW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376141"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="recA"
                     /locus_tag="VNN25_03935"
     CDS             complement(<1..1021)
                     /gene="recA"
                     /locus_tag="VNN25_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXH90708.1"
                     /translation="MATVPTDKLKAAETALTQIERQFGKGSIMRLGAKEFVPISAIST
                     GSIGVDAALGVGGVPRGRIVEIFGPESSGKTTLSLHVIAEAQKAGGLAAFIDAEHALD
                     AEYAKKLGVDIDNLLVSQPDSGEQALEIAEVLVRSGAIDVIVIDSVAALVPRAELEGE
                     MGDSHMGLHARLMSQALRKLTAIVSKSKTSLIFINQIREKIGVMFGNPETTTGGRALK
                     FYSSIRIDIRRTNQIKEGEEVVGSRVKVKVVKNKCAAPFRHAEFDVGYGEGISKTGEL
                     IDLGIENKIIDKSGSWFSYGDMRLGQGRENAKLFIKENPDLSGEIESKVRRAIGIGVP
                     ELTVIE"
     gene            complement(1156..1788)
                     /locus_tag="VNN25_03940"
     CDS             complement(1156..1788)
                     /locus_tag="VNN25_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524154.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HXH90709.1"
                     /translation="MKELETAAPLIPPRPTIKKLQAVAAECKACHLCENATQTVFGEG
                     KSSSTVMFIGEQPGDKEDLSGHPFVGPAGALLDKALVEAGIDRTKVYVTNVVKHFKWE
                     PRGKRRIHKKPNGAEIAACRPWLEAEIAVVKPLAIVCLGSTAAQAVISPKFRVSVQRA
                     QFVESSLAPLVTATVHPSSILRATSDEARHEEMQHFIADLKTIWKAISAR"
     gene            complement(1785..2984)
                     /locus_tag="VNN25_03945"
     CDS             complement(1785..2984)
                     /locus_tag="VNN25_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90710.1"
                     /translation="MDPAYRQTFNAAFTQERYEWYQRELSQRLNCTFEFRLAESPVFL
                     PDDFKAKIADAAQAIVDQLSDPSRLAEMKRAIPARWDTPGMDSLPTFTQVDFAVVREN
                     GVLVPKLIELQGFPSLTCLQVTQRDAWRDTMRSMEGLDSEWSCWFSGLDRESFIDLAR
                     RTITNGHDPAEVILMDIDPPRQKTYPDFAATKLLTGVDAVDPRTLTKRGKQLFRGNTR
                     VRRIYNRVVFDELISKGIDLPFDYREELDVEWAPHPNWYWVWSKYSLPFLDHPSVPRA
                     TFVSDLQKVPEDFSSRYVLKPLFSFAGGGVNVEPTAADLSAIPAADRHAWCVQEKIEY
                     EPALLAADGGGVKIEIRMMFLRPDDETKPILAQNLVRLSRGKMLGVDFNKQFTWVGSS
                     IALSQSS"
     gene            complement(2991..4004)
                     /locus_tag="VNN25_03950"
     CDS             complement(2991..4004)
                     /locus_tag="VNN25_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90711.1"
                     /translation="MNESTSLMTRRDIHESSDFFEYVRVCDSEPPADRDCIDVAILDM
                     NHSWPNAGHDSLVHAVLEAAEPLRETLAASGSKVRVLSYDVRRTQKVPQSPNGRFQLY
                     LGTGGPGHLDPRENDGVSEASQGIVESPAWEAPLFRLYDDIGAHDSAMLLGVCHSFGL
                     MCRWSGAARVELRAEKSSGMPENVLTRSAAAHPWFSQFANRLEDGRHFRVVDNRLFDL
                     VAEHSSDPNIFSREEEDGDGVTMIEFARSADGMPRILGVNHHPEIVDREHIMTVLEEK
                     RAHGEVTEAWYRERVETLRDLFVRFEKESRWTSEYTLLGPLRIQLTRLIHERTAETVA
                     QTV"
     gene            complement(4004..5113)
                     /locus_tag="VNN25_03955"
     CDS             complement(4004..5113)
                     /locus_tag="VNN25_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HXH90712.1"
                     /translation="MPPEPSFTLGIEEEFQVVDPETRQLRSHISEMFAEGEKRLKDEI
                     KREMHEAVIEVGTPICTDVNHARREVTRLRREIINLTRESGLRIAASGTHPISHWNSV
                     PITSNERYDKIVYDLQMVARANLIFGLHVHVAIEDPETRIQVMNAARYFLPHVFALSV
                     NSPFWCGQNTGWQSYRAKVFERFPRTGIPDTFRSASEYEEYVQLLIKTNCMDNAKKIW
                     WDVRPHPFFPTLEYRICDVPLRIDETICIAALFQAITYKLWSLYKRNMGWRVYRRMLL
                     NENKSRAARFGTHGKLIDFGRAEEVPYADLLEELLAFVDDVVDDLGSRDAVYYARTIV
                     ANGTGADRQLKVFEETNDLKAVVDYIISETEHGTA"
     gene            complement(5331..6290)
                     /locus_tag="VNN25_03960"
     CDS             complement(5331..6290)
                     /locus_tag="VNN25_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90713.1"
                     /translation="MRKKRIALVFGMEDTFPWALIDAINKRGGDQVEAMPAELSYLKD
                     DGVFPYDPILDRISHEIPFYRTYLKCAAASGVKIVNNPIWWSADDKFFDNLVAKSVGV
                     AVPRTVLLPHKDYPPSTEAKSFRNMKWVNWDELFAYLGFPIFMKPAYGGGWKDVYKCD
                     NQQEFFAAYDQTRDLTMMAQEAIEFDRYYRCYVIGRNRVHIMHYDPKQPHHLRYVKNP
                     PPMEPAFEARIRRDAIALCEALGYDMNTVEFAVRDGIPYAIDFMNCAPDADVASVGQD
                     NFDWVVSNMAEALIEIVTSGRKLELTGTWPSTLNTSKPVEQAI"
     gene            6492..7199
                     /locus_tag="VNN25_03965"
     CDS             6492..7199
                     /locus_tag="VNN25_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXH90714.1"
                     /translation="MYREHVFWHSPSLGRDMEFAWYGKFGRPVMLFPTSAGRYSENED
                     RGIPESLADKVNGGEIQLILVDTVNDESWYNRHVHPAVRAARHAQYDAYLRHELVPYI
                     FNRAQRGDLAVYGASFGAYHAANFAARYPDVVSRAICFSGIYDIHDQLDGYWDENCYF
                     NCPTAYIPNMDGEWVGKLSRIDWIIATGEYDTIVDKNREFSAILWSKGIQNYLEIWGG
                     VFGHDWPWWRENLRRFL"
     gene            7232..>9851
                     /locus_tag="VNN25_03970"
     CDS             7232..>9851
                     /locus_tag="VNN25_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH90715.1"
                     /translation="MRRFAVAALVVAVAVAAHAQLDFDGRLQIEMRDGSRVAIPQQAE
                     AANGQLVEFVAPPAAISAGKTAVDYQATFTRFRDDLSRIVNAGRAAKTAVTPEIRREY
                     FVVFNGAAIDVPQELVAQIRALPYVKRVEADRTVHAFASANISLVGADKVWTTLSSRG
                     KGVTVAIIDTGIDYMHPALGGGFGPGFKVIGGWDFVNNDADPFDDNGHGTHVSGIVAG
                     DSSTITGVAPDASLIAYKVLGSNGSGSDSNVLAAVERAADPNGDGNTNDHADVANLSL
                     GGGGNPDDPLSEAIDNASAAGVTFAIAAGNSGTFHSISSPGTARSAITVGASDLNDLI
                     AGFSSRGPNTKNLAIKPDVVAPGLSILSSYPGNRYVTLSGTSMATPHVAGAAALLKSL
                     HHDWTPAQIKQALMNNAKPVLDEIMAGGQGRIDAYAAATSNLVIDPPSISLGLLPADQ
                     TNWSVTRTLHLTNRGTTTLSYTVKSNAAFGEIVTPSSPTVTIPAGGSADVTLTFTFSV
                     GTVQFSTNSFTGGGQVVLANTAVPADSRNIQFAFIKAARETAHSDRAYPDAVWVSADR
                     TRTAGATFIDDNTSETLMTPGTWDMLIWTREADPKTNAIVATDFIAREKVAVAGDGAI
                     SLTAADAPHTITFAGRRENGQPLTGTGYLNSGRIVLDTTQPGGVTSLSFPWTSAPVMH
                     ISDITAQKTLLFYETVLDTEASEYYMVQHPPLTGVSDDATLTTGGSALRGGTVEIGVP
                     PPVRGDRRLVAVATNPTLSTGVSATGIIDAPIVSARVFVSPDVNPSYVNGFAFIVVTD
                     GITQYTTPLLRVIDDKLVSVGAGSVLPWTYSGSTYEYGLGPRFPLSIFATPSGTSRMS
                     LSTDIVGPLGESRVTDR"
BASE COUNT         1899 a         2959 c         3047 g         1946 t
ORIGIN      
        1 cttcgatcac cgtcagctcc ggtacgccga tgccgatggc gcggcggacc ttggattcga
       61 tctcgcccga aaggtcgggg ttctctttga tgaagagctt ggcgttctcg cggccctgcc
      121 cgagacgcat gtcgccgtac gagaaccacg agcccgactt gtcgatgatc ttgttctcga
      181 tgccgaggtc gatgagctcg ccggtcttgg agatgccttc gccgtaacct acgtcgaact
      241 cagcgtgccg gaacggtgcg gcgcacttgt tcttgacgac tttgaccttc acacgcgagc
      301 cgacgacttc ctcgccttcc ttgatctggt tcgtgcggcg aatgtcgatg cggatggatg
      361 agtagaactt cagggcccgg ccgcccgtgg tcgtttccgg gttgccaaac atcacaccaa
      421 ttttctcacg aatctggtta atgaaaatga gtgatgtttt cgacttcgaa acgattgctg
      481 ttaatttgcg aagagcttgt gacattaaac gggcgtgtaa tcccatatga gaatcaccca
      541 tctcgccttc gagctccgcg cgcggcacga gcgccgcgac ggagtcgatg acgatgacgt
      601 cgatcgcgcc gctgcgcacc agtacttcgg cgatctcgag cgcctgctcg ccgctatccg
      661 gctgcgatac gagcaggttg tcgatgtcca cgccgagctt cttcgcgtac tcggcgtcga
      721 gggcatgctc ggcgtcgatg aacgcggcca gtccgcctgc tttctgcgct tctgcgatga
      781 cgtgaagcga gagtgttgtt ttacccgatg attccgggcc gaagatctcg acgatccggc
      841 cgcgcgggac gccgcccacg ccgagcgccg cgtcgacgcc gatggatccg gttgagatcg
      901 cgctgatcgg cacgaattct tttgcgccga ggcgcatgat cgagcctttg ccgaactggc
      961 gctcgatctg cgtcagcgcg gtttcggccg ctttcaattt gtctgttgga actgtggcca
     1021 tagctggcta ctccttcgat tcgaatcggt ggatggaaag gtagtgacgc gtgctcggat
     1081 cgccggcatt cgtgtggtgg agcgagtgta cgacggcatt ttttgaaccg tcaagaaact
     1141 ctacagttcg tggatttatc gggccgaaat cgccttccag atcgtcttca ggtcagcaat
     1201 gaagtgctgc atctcctcgt gacgcgcctc atcgctggtt gcgcgaagga tcgacgacgg
     1261 atgcaccgtg gcggtgacga gaggagccag actggattcg acgaactggg cgcgctgcac
     1321 ggagacacgg aacttcggac tgatgacggc ctgtgccgcc gtcgacccga gacagacgat
     1381 agccagcggc ttcacgacgg cgatctcggc ctcgagccag ggacggcaag ccgcgatctc
     1441 ggcaccgttc ggcttcttgt ggatacgccg cttgccgcgc ggttcccatt tgaagtgctt
     1501 cacgacgttg gtgacataga ccttcgtgcg gtcgatccct gcttcaacga gagccttgtc
     1561 gagcagggcg ccagccgggc cgacgaaggg gtggccgctg agatcctctt tgtcgccagg
     1621 ctgctcgccg atgaacatca cggtggagga tgatttgcct tcgccgaaaa ccgtctgggt
     1681 ggcgttctcg cagagatgac acgctttgca ttcggcagcg accgcctgaa gcttcttgat
     1741 cgtcggccgc ggcgggatca gcggtgcagc ggtttcgagc tccttcacga cgactgcgag
     1801 agtgcaatcg acgaacccac ccaagtgaac tgcttgttga agtcgacacc gagcatcttg
     1861 cctcgggaga gtcgcaccag gttctgcgcg aggatcggct ttgtctcgtc gtcggggcga
     1921 aggaacatca ttcggatctc gatcttgact ccaccaccgt cggccgccag cagagccggc
     1981 tcgtactcga tcttctcctg cacgcaccag gcgtgacggt cggcggctgg aatggcgctg
     2041 aggtcggcgg cggtcggctc gacattgacg ccgccgccgg cgaaggaaaa gagtggcttc
     2101 aggacatagc gcgaggagaa atcctccggc actttctgga ggtcggacac gaacgtagct
     2161 cgcggcacgg aagggtgatc gaggaacggc agcgagtatt tcgaccatac ccaataccag
     2221 ttgggatgcg gagcccattc gacgtcgagc tcttcgcggt aatcgaacgg cagatcgatc
     2281 cccttgctga tcagctcgtc gaagacaacg cggttgtaga tgcgccgcac gcgcgtgttt
     2341 cctcggaaga gctgtttccc tcgcttcgtc agcgtgcgcg gatcgacagc gtcaacaccc
     2401 gtgagcaact tcgtggccgc aaagtcgggg tacgtcttct gcctcggtgg atcgatgtcc
     2461 atgaggatca cttcggcagg atcgtgccca ttcgttatcg tgcggcgggc gaggtcgatg
     2521 aatgattctc gatccagacc tgagaaccag caagaccact cggaatcgag tccttccatc
     2581 gatcgcatgg tgtcgcgcca ggcgtcgcgc tgcgtgactt gaaggcaggt cagcgacgga
     2641 aaaccctgca attcgatcag cttcggaacg agcacgccgt tttcgcgcac gacggcgaag
     2701 tcgacctgcg taaacgtagg caagctgtcc atgccgggcg tgtcccatcg cgcaggaatg
     2761 gctcgtttca tctcggctag ccgcgacgga tcggagagct gatccacgat cgcctgcgcc
     2821 gcgtcggcga tcttcgcttt gaagtcatcc ggcaagaaca ccggcgactc ggcgagcctg
     2881 aactcgaacg tgcaattcaa gcgttgcgac agctcgcgct gataccactc gtagcgttcc
     2941 tgcgtgaacg cggcattgaa ggtctgtcgg tacgcagggt ccatggcaaa tcagacggtc
     3001 tgcgcaactg tctcggccgt tcgctcatga atcagccgcg tgagttgaat ccgcagaggg
     3061 ccgaggagcg tgtactcgga ggtccagcgg ctctccttct cgaagcggac gaagagatcg
     3121 cgcagcgtct cgacgcgctc gcggtaccag gcctcggtga cctcgccgtg cgcgcgcttt
     3181 tcttcgagca cggtcatgat gtgctcgcgg tcgacgatct cggggtggtg attgacgccg
     3241 agaattcgcg gcatgccgtc agcgctgcgc gcgaactcga tcatcgtcac gccgtcgccg
     3301 tcttcctctt cgcgggaaaa gatgtttgga tccgaggagt gttcggcgac gagatcgaag
     3361 agacggttgt cgactacgcg gaagtgacgg ccatcctcca gacgatttgc gaactggcta
     3421 aaccatggat gggcggcggc gctccgcgtc aagacgttct ccggcattcc gctgctcttt
     3481 tccgcgcgaa gctcgacgcg tgcggcaccg gaccagcggc acatgagacc gaagctgtgg
     3541 cagacaccca ggagcatcgc ggagtcatgg gcgccgatgt cgtcgtacag gcggaagagc
     3601 ggtgcctccc acgccggact ctcgacgatg ccctggctgg cctccgacac accgtcgttt
     3661 tcccggggat cgagatggcc ggggccgccg gtgccgaggt agagctgaaa ccggccgttc
     3721 ggcgattggg gcaccttctg cgtgcggcgg acgtcgtacg agagtacacg gactttcgaa
     3781 cccgaggctg caagcgtttc gcgcaacggc tccgcggctt cgagtacggc atggacgagt
     3841 gaatcgtgac cggcgttggg ccacgaatgg ttcatgtcga ggatggcgac gtcgatgcag
     3901 tcgcggtcgg cggggggctc cgaatcgcag acgcgaacat attcgaagaa atcactcgac
     3961 tcgtggatgt cgcggcgtgt catcagactg gttgactcat tcatcaggcg gttccgtgct
     4021 ccgtttcgct gatgatgtag tcgacgacgg ctttgaggtc gttcgtctcc tcgaagacct
     4081 tcagttgacg gtcggcaccg gttccgtttg caacgattgt gcgtgcgtag tagacggcgt
     4141 cgcgggagcc gaggtcatcg acgacgtcgt cgacgaacgc cagaagctcc tcgagcaggt
     4201 cggcgtaggg gacttcctcc gcccggccga agtcgatgag ctttccgtgc gttccgaacc
     4261 tggcagcacg cgacttgttt tcgttgagca gcatgcggcg gtagacgcgc caacccatgt
     4321 tccgcttgta aaggctccag agcttgtagg tgatggcctg gaagagggct gcgatgcaga
     4381 tggtctcgtc aatcctcaac ggcacgtcgc aaatccggta ttcgagcgtc gggaagaagg
     4441 gatgtggccg gacgtcccac cagattttct tggcgttgtc catacagttc gtcttgatga
     4501 ggagttgtac gtactcttca tattcgctgg cggagcgaaa cgtgtcggga atgccggttc
     4561 ggggaaaacg ctcgaagacc ttggcgcggt agctttgcca cccggtgttc tggccgcacc
     4621 agaaaggtga gttgaccgaa agagcgaaga catgcggcag gaagtaccgg gccgcgttca
     4681 tcacctggat gcgcgtctcc gggtcctcga tggcgacgtg gacatgcagg ccgaaaatca
     4741 ggttggcccg tgccaccatc tgcaagtcgt agacgatctt gtcgtagcgc tcattggacg
     4801 tgatggggac gctgttccag tgagagatcg gatgtgttcc cgaggcggcg atgcgcaggc
     4861 cgctctctcg cgtcaggttg atgatctcgc gccgcagcct tgtgacttct cgccgggcgt
     4921 gattgacgtc ggtgcagatc ggcgtgccga cctcgatcac cgcctcgtgc atctcccgct
     4981 tgatctcgtc cttgagccgc ttttcccctt ccgcgaacat ctcggagatg tgcgaccgca
     5041 gttggcgcgt ttccggatcg acaacctgaa actcctcctc gatcccgagg gtgaaggaag
     5101 gttctggagg catggcggaa ggatatcagg ggaagagaga ggcagagatc aggaaaatca
     5161 gagatcagag atattagaga tcagagcagg agaaccgcga tacttctgat ctctgatttc
     5221 cagtctctgt ttgagatcgg aagcgaggcg cagagatcag gaaaatcaga gatcagacca
     5281 gtagacgggc acactcctgg tctcttgcct ctgatctcct atctcagctc tcagatcgcc
     5341 tgctccaccg gcttgctcgt attcagcgtc gacggccagg ttcctgtcag ctccaacttg
     5401 cggccgctgg tcacgatctc gatcagcgcc tcggccatgt tggacacgac ccagtcgaag
     5461 ttgtcctggc caacggacgc cacatcggca tcgggcgcgc agttcatgaa gtcgatggcg
     5521 taaggaatcc cgtcccgcac ggcgaactcg acggtgttca tgtcgtagcc gagcgcctcg
     5581 cagagggcga tggcatcgcg gcggatgcgg gcctcgaacg ccggttccat cggcggtggg
     5641 ttcttcacgt agcgcagatg gtggggctgt ttcgggtcgt aatgcatgat gtgaacacgg
     5701 ttgcggccga tgacgtagca gcggtagtag cggtcgaact cgatggcctc ctgggccatc
     5761 atggtcagat cgcgggtctg gtcataggcg gcgaagaact cctgctggtt gtcgcacttg
     5821 taaacgtcct tccagccgcc gccgtacgcc ggtttcatga agatgggaaa gccgaggtag
     5881 gcgaagagtt cgtcccagtt gacccatttc atgttgcgga aggacttggc ttccgtgctc
     5941 ggcggatagt ctttgtgagg aagaagcacc gtgcggggaa cggcgacgcc gaccgatttg
     6001 gcgacgaggt tgtcgaagaa cttgtcatcc gcagaccacc agatcgggtt gttgacgatc
     6061 ttgacaccgg aggcggcggc acacttgagg taggtgcggt agaacgggat ctcgtggctg
     6121 atgcgatcga ggatcgggtc gtaggggaag acgccgtcgt ccttgagata cgacaactcc
     6181 gctggcatgg cctccacctg gtcgccgcca cgcttgttga tggcgtcgat caacgcccag
     6241 ggaaatgtgt cttccatacc gaacacgagc gcaattcgct tttttctcat cgaaatctcc
     6301 gggcggcgga tcgtatagaa tcggcggccc agccgcaaat ggggcttgac gtaaaggctg
     6361 aatgttgaac gttcaatgtt gagtgattaa tgttgaatga aaactgcatc gattcgatca
     6421 aatgaatagc tgacgattaa tagcgaatcc aattcaacat ttaacagtca ccattcaagg
     6481 tcatcctttc tatgtatcgc gagcacgtct tctggcacag cccgtccctc ggccgcgaca
     6541 tggaatttgc ctggtacggc aagttcggac gcccggtcat gctctttccc acctcggcag
     6601 gccgatattc tgaaaacgag gaccgcggca tcccggagtc gctcgcggac aaagtgaacg
     6661 gcggcgagat ccagctaatc ctcgtcgaca ccgtgaacga cgagagctgg tacaaccggc
     6721 acgtgcatcc tgccgtccgg gcggcgcggc acgcgcagta cgacgcctat ctccgccacg
     6781 agttggtgcc gtacatcttc aaccgggcac agcgaggcga tctggcagtc tacggcgcca
     6841 gcttcggcgc ctatcacgcc gcgaactttg ccgcccgcta tcccgacgtc gtcagccgcg
     6901 ccatctgttt ctccggcatc tacgacattc acgaccagct cgacggctac tgggacgaga
     6961 actgctactt caactgcccc accgcgtaca ttccgaacat ggacggcgag tgggtcggca
     7021 agctctcgcg catcgactgg atcatcgcta ccggcgagta cgacacgatc gtcgacaaga
     7081 accgcgagtt ttcggcgatt ctctggtcga agggaattca gaattacctc gagatctggg
     7141 gaggcgtctt cggccatgac tggccgtggt ggcgtgagaa tctccgccgc ttcctctgaa
     7201 acgcacgaag ttagcaggtc agggagccca tatgcgtcgt ttcgcagtgg cagcgttggt
     7261 cgttgcggtg gccgtcgccg cccacgcgca gctcgatttc gatggccgcc tccagatcga
     7321 gatgcgcgat ggttccaggg tcgccattcc gcagcaggca gaggcggcga acgggcagct
     7381 cgtcgagttc gtagcaccgc ccgctgcgat cagcgcggga aagactgccg tcgactacca
     7441 ggcgacgttc acccgcttcc gggatgatct gagcaggatc gtcaacgccg gacgcgctgc
     7501 caagaccgcg gtcacgccgg agattcgccg cgaatacttc gtcgtcttca acggcgccgc
     7561 gatcgacgtt ccgcaagagc tggtcgcgca gatccgggca ttgccgtacg tgaagcgcgt
     7621 cgaggcggac cggacggtgc atgcgttcgc gagtgccaac atctctctcg tcggtgcgga
     7681 caaagtctgg acaaccttga gctcacgcgg gaagggcgtg accgtcgcca tcatcgacac
     7741 cgggatcgac tacatgcatc cggcgctcgg cggcggtttc gggccgggat tcaaggtgat
     7801 cggcggatgg gacttcgtaa acaacgacgc cgatcccttc gacgataacg gacacggaac
     7861 gcacgtctca ggaatcgtgg ccggagactc gtccaccatc acgggcgtag ctcccgacgc
     7921 gtcgctcatc gcgtacaaag ttctcggttc gaacggaagc ggcagcgatt ccaacgttct
     7981 cgctgccgtc gaacgtgccg ccgatcccaa cggagacggc aatacgaacg atcacgccga
     8041 cgtggccaac ctcagcctgg gaggaggcgg caacccagat gacccactct cagaggccat
     8101 cgacaacgcc tccgcggccg gagtcacatt cgccatcgcc gcgggcaaca gcggaacatt
     8161 tcacagcatc tccagcccgg ggaccgcgcg gagcgccatc accgtcgggg ccagcgatct
     8221 gaacgacctc atagccggtt tttcctcgcg cggtccgaac acgaagaacc tcgccatcaa
     8281 accggatgtg gttgcgcccg gcctttcgat tctctcgtcg tatcccggca accggtatgt
     8341 gacgctgagc ggaacgtcga tggccacgcc gcatgtggcc ggagcggctg cgctgctcaa
     8401 atccctgcac cacgactgga cgcccgcaca gatcaaacag gctctcatga acaacgccaa
     8461 gcccgtcctc gacgagatca tggccggcgg ccagggacgc atcgatgcat acgcggcggc
     8521 cacaagcaac ctcgtcatcg atcctccgtc gatcagtctc ggacttctgc cggccgacca
     8581 gaccaactgg tcggtgacgc gcacgctgca cctgacgaat cgaggaacga cgacgctgtc
     8641 ctacacggtc aaatcgaacg ccgctttcgg cgagatcgtg acgccttcgt cgccaacggt
     8701 aaccatccca gccggtggct ccgcggatgt aactctcaca ttcacattca gtgtcggaac
     8761 ggtgcaattc agcacgaact ccttcactgg cggcgggcag gtcgtgcttg caaacacagc
     8821 cgtgccggcc gactcccgga acattcagtt tgcgttcatc aaagcggcgc gggagacggc
     8881 gcactccgac cgggcctacc ccgacgcggt ctgggtcagc gcggaccgga ccaggactgc
     8941 aggtgcgact ttcatcgatg acaacacatc cgaaaccctg atgacccccg gcacctggga
     9001 catgctgatc tggacgaggg aggccgaccc gaaaacgaac gcgattgtgg ccaccgactt
     9061 catcgcccgc gagaaagtcg ctgttgccgg cgacggcgct atctcgctga cggccgcaga
     9121 cgcacctcac acgatcacat tcgcggggcg acgcgagaac ggtcagccgc tgaccggcac
     9181 cggatacctg aacagtggcc gcattgtgct ggacaccact cagccgggcg gagtcacgtc
     9241 gctctcattt ccgtggacca gcgcaccggt gatgcacatc tccgacatca ccgcgcagaa
     9301 gacgctgctg ttttacgaaa ccgtgctgga cacggaagca agcgagtact acatggtcca
     9361 gcatccaccg ctgaccggcg tgagcgatga cgcgacgctg acgaccggag gttcggcgct
     9421 gcgcggcggg accgtcgaga tcggcgtacc accgcccgta cgaggcgatc gccggcttgt
     9481 cgctgtcgca accaacccga cgctctcgac cggtgtctcg gccaccggaa tcatcgacgc
     9541 accgatcgtg agcgcgcggg tgttcgtctc gcccgacgtc aatccgagtt acgtcaacgg
     9601 attcgcgttc atcgtcgtca ccgacggcat cacccagtac acgacaccgt tgctgcgcgt
     9661 gatcgacgac aaactcgtta gcgtcggagc aggcagcgtt ttgccgtgga cctacagcgg
     9721 ttccacctac gaatacggcc tcgggccccg attccctcta tcgattttcg ctactccgtc
     9781 cggaacgtcg cgcatgagtt tgtcgacgga tatcgtcggt ccgctcggtg agtcgcgcgt
     9841 taccgatcgc a
//
LOCUS       DATLVW010000121         3469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_500586, whole genome shotgun sequence.
ACCESSION   DATLVW010000121 DATLVW010000000
VERSION     DATLVW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500586"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VNN25_03975"
     CDS             complement(<1..410)
                     /locus_tag="VNN25_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90716.1"
                     /translation="MSGQDWSITIVSLDNEGDPSKPSAAFQPDLMGAKPGDPLKAGNA
                     DLISWNNRTSNEHQPWAANAQYQPLPESQIVKIDANGNPGNYLSDPIPPWQPSSPAFT
                     TYIPTDANGNPITNPVIVYYVCKFHPDEQGQIIV"
     gene            complement(607..1194)
                     /locus_tag="VNN25_03980"
     CDS             complement(607..1194)
                     /locus_tag="VNN25_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90717.1"
                     /translation="MVYRNIDVIDRDIDVVYRDIDVENRDIAVINRDIAVINRDIDVE
                     NLDFDVENRDCDVENLDIAVRDLDIAVVNRDIDVENLDFVVENRHCDVENLDTAVRDL
                     DIDVEIHDIDVENLDTAVRDLDIAVRNLDIDVENLDVVVVNLEPPGSDGELTRSDGEP
                     GVRALRMPWIDRQPRRRRGRDQIITLGIAMAVLGI"
     gene            complement(1366..2028)
                     /locus_tag="VNN25_03985"
     CDS             complement(1366..2028)
                     /locus_tag="VNN25_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90718.1"
                     /translation="MNGITWTLVLAAATVGSLHTTAPDHWMPFAALARARGWSPARTA
                     RTTILCGLGHVTVSAALGIAAAFAGLSVIHLLGGRFQSIGNALLIAFGTVYMIWGIRR
                     SLRRDPLAPTHAHDHRHIQGHNDHDHGLTEWSLFLLFSADPCVAVVPMVMAAAGVGWR
                     TTGAVVASYELATIGTMVVLVTAAHAGVRALRMPWIDRHSHAVAGAVIITLGIAMAVL
                     GI"
     gene            complement(2025..2735)
                     /gene="hypB"
                     /locus_tag="VNN25_03990"
     CDS             complement(2025..2735)
                     /gene="hypB"
                     /locus_tag="VNN25_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HXH90719.1"
                     /translation="MCQDCGCGDVNLVPLELQTRILARNDSTAEHNRQHLRERGIFTV
                     NLMGSPGSGKTAVLESTARIFKDGRRLAAIAGDLATDNDAARLVRAGIRSCAIETGQA
                     CHLDAEMVHHELHHLDLEGVEFLFIENVGNLVCPAVYDLGQSVNVVALSVTEGEDKPL
                     KYPTMFAKADLVLLTKLDLLPHLPRVDINAIRNAIAKVMPDPHIIRMSAQDDVGVDGW
                     VAWLRARRAALMHETVPA"
     gene            complement(2735..3076)
                     /locus_tag="VNN25_03995"
     CDS             complement(2735..3076)
                     /locus_tag="VNN25_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXH90720.1"
                     /translation="MHEYSIVQSLVDSVAAAVGTRTDAVVRSVRVSIGEVAGVDCGLL
                     TTAYEVFRAGTLCENAAMTIDRIPARWECPLCGAAIARGAVLQCPQCHEPAFMAAGDE
                     IILQRVELEVA"
     gene            complement(3076..>3469)
                     /locus_tag="VNN25_04000"
     CDS             complement(3076..>3469)
                     /locus_tag="VNN25_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXH90721.1"
                     /translation="WAYAKTGLTGAESSTFMLPRLVGLRRAFELLFLNPRITPERALE
                     IGLITAVYPAATFDAEVDAIAHKIAEGPTEAMGIAKELINQAAGMDRLDVHLDREIDH
                     LARIADGADFAEGIDAFFAKRQPKFGVS"
BASE COUNT          666 a         1064 c         1068 g          671 t
ORIGIN      
        1 ctgacgatga tctggccctg ctcgtcagga tggaacttgc agacgtagta gacgatcacc
       61 ggattcgtga tgggattgcc gttggcgtcg gttggaatgt aggtcgtgaa ggccggcgat
      121 gagggctgcc acggcggaat cggatccgac aggtagttgc ccggatttcc gttcgcatcg
      181 atcttcacga tctggctttc gggcaatggc tgatactgcg cgttggccgc ccacggctgg
      241 tgctcgttgg acgtccggtt gttccacgag atcaaatcgg cgttccccgc tttcaacgga
      301 tctcccggct ttgcgcccat caaatcgggt tgaaacgccg ctgaaggctt agagggatct
      361 ccttcgttat caagggaaac aatcgtgatc gaccagtctt gaccgctcat ggcattcctc
      421 catttcgggc gcgccgaggc acgcctccgt tccaggcaca tcggattgag atgttcgaag
      481 agactaccca aataggagag tccgcaccgg aaagtttccc tacccgaatt ctccgcgtcc
      541 tccgtgtctc cgcgtgaaac gcatccaaaa ccttgtcacg cggaggcgcg aaggacgcgg
      601 aggggatcag atccccagca cggccatggc gatgccgagg gtgatgatct gatcacggcc
      661 ccggcgacgg cgtggctggc ggtcgatcca gggcatgcgc agcgcgcgga cgccgggttc
      721 accgtcggag cgggtcaact caccgtctga gccgggcggc tcaagattga ccacgacgac
      781 atcgaggttc tcgacatcga tgtccagatt tctaactgcg atgtccagat ctctaactgc
      841 tgtgtccaga ttttccacat cgatgtcatg aatttccaca tcgatgtcca gatctctaac
      901 tgcggtgtcc agattttcca catcgcagtg tcgattttcc acaacgaagt cgagattctc
      961 cacatcgatg tctcgattga ccactgcgat gtccagatct ctaactgcga tgtccagatt
     1021 ttccacatcg cagtctcgat tttccacatc gaagtcgaga ttttccacat cgatgtctcg
     1081 attgatcact gcgatgtctc gattgatcac tgcgatgtct cgattttcca catcgatgtc
     1141 tcgatagacc acatcgatgt ctcgatcgat cacatcgatg tttcgataga ccacatcgat
     1201 gtcgagattc gacccatcgc agtctcgatt gatgacatcg aagtcgagat tcgacctatc
     1261 gatgtccaga ttttccacaa cgatgtcgag agtcgacaca tcggcctcgc gcgcgggcgg
     1321 ctcaccgtct gagcgtggaa gacagcgatg tgaggcgccg gctcgtcaga tccccagcac
     1381 cgccatcgcg atgccgaggg tgatgattac ggcgccggcg acggcgtggc tgtggcggtc
     1441 gatccacggc atgcgcagcg cgcggacgcc ggcgtgggcg gccgtgacga ggacgaccat
     1501 cgtcccgatc gtcgccagtt cgtacgaggc gacgacggcg ccggtcgtgc gccagcccac
     1561 tcccgccgcg gccatgacca tcggaacgac ggcgacgcac gggtcggcgg aaaagaggag
     1621 gaacagcgac cactccgtga ggccgtgatc gtgatcgttg tgtccctgga tgtggcgatg
     1681 atcgtgcgca tgagtcggcg ccagcggatc gcggcgcagg ctgcggcgga ttccccagat
     1741 catgtagacc gttccgaacg cgatcagcag cgcgttaccg atcgattgaa accttccgcc
     1801 gaggaggtga atcacgctca gaccggcaaa ggcggcggcg atgccgagtg ccgccgacac
     1861 cgtcacgtgt ccgagcccgc acagaatcgt tgttcgtgcc gtgcgtgcag gactccagcc
     1921 tcgagcccgc gccagcgcgg cgaacggcat ccagtgatcg ggtgccgtcg tgtgcagcga
     1981 tcccaccgtt gccgcggcca ggacgagcgt ccaagtgatc ccgttcatgc cggcacggtc
     2041 tcatgcatga gggctgcccg ccgcgcacgc aaccaggcca cccagccatc gacgcccaca
     2101 tcgtcctgcg ccgacatgcg aatgatgtgc ggatcgggca tgaccttggc gatcgcgttg
     2161 cggatcgcgt tgatgtccac cctcggcaga tgcggcagca gatcgagttt cgtcaggagc
     2221 acgagatcgg ctttcgcaaa catggtcggg tatttgagcg gcttatcctc tccctcggtc
     2281 accgaaagcg caacgacgtt gacgctttgg ccgaggtcgt agacggccgg gcagacgaga
     2341 ttgccgacgt tctcgatgaa gagaaactcg acgccttcga gatcgagatg atgcagctcg
     2401 tgatgcacca tctccgcatc gagatggcag gcctggccgg tctcgatcgc gcacgagcgg
     2461 atgccggcgc gcacgagccg cgcggcgtca ttgtcggtgg caagatcgcc ggcaatcgcg
     2521 gcgaggcggc gtccgtcttt gaagattcgc gccgtcgact cgagcaccgc ggtctttccc
     2581 gaaccgggcg agcccatgag gttgacggtg aagatgccgc gctcacgaag atgctggcga
     2641 ttgtgttcgg ccgtgctgtc gttgcgcgcg aggatgcgcg tctgcagctc cagcggaacg
     2701 aggttgacat ctccacatcc gcagtcctga cacatcaggc cacctccagt tcaacgcgtt
     2761 gcaaaatgat ctcgtcgccc gccgccatga atgccggctc gtggcattgc ggacattgca
     2821 ggaccgcacc gcgcgcgata gcggcgccgc agagcgggca ctcccaacgc gccggaatgc
     2881 gatcgatcgt catcgcggcg ttctcgcaaa gcgtgccagc ccggaacact tcgtaagcag
     2941 tggtgaggag gccgcaatcg actcccgcaa cctcgccgat gctgacgcgc acgctgcgaa
     3001 cgaccgcgtc ggtccgcgtt ccaacagccg cggcaacgga gtcgacgagc gactggacga
     3061 tggagtattc gtgcatcacg acactccgaa tttcggctgc cgtttggcaa agaaggcgtc
     3121 gatgccttcc gcgaaatcgg cgccgtcggc gatgcgcgct aggtggtcga tctcgcggtc
     3181 gaggtgaacg tcgagccggt ccatccccgc ggcctggttg atcagctctt tcgcgattcc
     3241 catcgcctcg gtcggccctt cagcgatctt gtgtgcgatc gcatcaacct cggcatcgaa
     3301 ggtcgcggcc ggatagaccg cagtgatcaa gccgatctcg agcgcgcgct cgggcgtaat
     3361 gcgaggattg aggaagagga gctcgaaggc gcggcgaaga ccgacgagac gcggcaacat
     3421 gaaggtcgag ctctccgcgc cggtcaatcc cgtcttcgcg tatgcccac
//
LOCUS       DATLVW010000122         3880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_131015, whole genome shotgun sequence.
ACCESSION   DATLVW010000122 DATLVW010000000
VERSION     DATLVW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131015"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VNN25_04005"
     CDS             complement(<1..537)
                     /locus_tag="VNN25_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90722.1"
                     /translation="MNSRNFHILKYHVRVRRLSLLIPILLGIALHASAFSGRTLSPLR
                     FRDQRIDYSASIATDGQSFLVVGVYGWFPYSIYGRIITAQGVSGKPFFISPGDHAEAV
                     WNGHAYLVIVQDADYSVDVVTIPQSGGGVVSVKHVWEDTPAHRQWLAVGWNGRRVILT
                     SNDTPQDQTGGHGRVQMLD"
     gene            complement(974..>3880)
                     /locus_tag="VNN25_04010"
     CDS             complement(974..>3880)
                     /locus_tag="VNN25_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXH90723.1"
                     /translation="LFAPSAFPFGQNKIVYDKFHWNVYHATHFDIYFYDEEKPALEKV
                     VDMAESAYDDLSRKFNFQVSKKIPIIYYATHSAFEQTNVDLNFIPEGVGAFAEPTRNR
                     MVIPIDTTDEKLLQLVQHELTHVFEYEILFQGKLGKEVTSNPPTWMMEGLASFMGNDE
                     DSKDRMVLRDAVVNDAVPPITHAQGGGYFAYRFGHAVFAFIQQKYGWEGLRDFIYEYR
                     NTLGNSVDKALKRAFDVKPDEFDSQFRTWLRKQYLPALVSKGEPQEYGDSFKISEGTE
                     SEDMSPSPSPSGDLLAAFATYKEDVDVILYNIPERRLLRNLTSGFTTKYEYAIAQMLT
                     VGPVMGRDVGFSPNGDQIALFVKKERGRNLLLINALNGHIDRSVKMDFEQQLAPTYSP
                     DGKTIAFAGFKGNVPDIYLYDIGSGAIRNLTNDKFFDGAPVFSPDGKWLLYSSVVNGY
                     AKLFKLNLANPSERFQLTTGEWNDIDSWFSPDGKRIFFASDKQTGRNIAAAVEILQNA
                     EDKAKPESATPPVDLTKYAAYNIYSLNLDNGDLLQYTDVVGGCFTPVVFTGTNNRERM
                     VFASYYKRHWRLYSTTTDKPLHAAEKTVLPSAPMLASARPFFQPPVEVTIDPDKIETV
                     NSHKLFIDDVEVNAGVTSDNLFLSRSVIFMSDMLGDRRFIASLDSVSSFSNFDFLYLD
                     LHNRTNWGFRIFDSRTFFTTLSTTSDFTLDRRQLYRETGFVGILSHPFDRYHRVDAGM
                     GYEERDVNYPFIDDAGDVFFVNRRDNFPFVTASFTGDTTSFKEFGPIAGHRYELNTVF
                     APNVHRSAGTSGVLTNDYTIDARQYLPISSRALLATRVFAGYSHGSFPNFFYFGGLNT
                     VRGFEYRSIVGTKAGYANAELRFPLIDVLVTPVIAVTGIRANLFLDVGGASLPGQPYV
                     FWKNHRLVDGVASVGYGISFNLLGLELHLDFAKRTDLRSTNGKFHSEFWIGEVF"
BASE COUNT          845 a         1098 c         1143 g          794 t
ORIGIN      
        1 gtcgagcatt tgtactctgc cgtggcctcc cgtttggtct tgaggcgtat cgttggacgt
       61 cagaatcact ctacggccgt tccaacctac cgccagccat tggcggtggg ccggcgtatc
      121 ctcccaaacg tgtttgaccg agaccacgcc gcccccactc tgaggaatcg tgacgacatc
      181 gacgctatag tcggcatcct gaacgatgac cagataggcg tgtccgttcc agaccgcctc
      241 ggcgtgatca ccagggctga taaagaacgg cttaccggaa actccctgag ccgtaatgat
      301 tcgcccatag atgctgtacg gaaaccagcc gtagacccct acgaccagaa aactctggcc
      361 atccgtagcg atcgacgccg agtagtcgat cctttgatcg cggaagcgga gcggtgacaa
      421 ggttctgccg gaaaatgccg aggcatgcag agcgatacca agaaggatcg ggatcagtag
      481 tgacagccgg cgcacgcgaa catgatattt caggatgtgg aagttgcggg aattcacggg
      541 gccggccacg ccggtgagga atcgttatcg cgccggccgc agagtctccc acgagaacag
      601 cgtgtcggga acgcggttca caacacgttc ccgtcttccc actacgatga ggcggcgtgg
      661 ctcggattca aggggcgccc cgctgtcacg cctctcggaa tgcagggcaa tcccccctta
      721 gaacacctct ccgatcactc ttctcgagaa ggctatcgcc gaagcatcca aactgccgga
      781 cgaagaacag aacgcgttcg cggcgtgggc attggctgag ctggagtccg agcgccgttg
      841 ggatgatctc tttgcacgct cgcaggatct gctttcacgc ctcgccgacg aagcgcacga
      901 ggactacctt gctggtcgaa cggaaccact cgaccttgaa acgttgtgaa gtcgcaaacg
      961 ctcccgccct cccttagaac acctctccga tccagaactc cgaatggaac ttgccattgg
     1021 tgctgcgaag gtccgtccgt ttggcgaagt ccaagtgcag ttcgagaccc agcagattga
     1081 atgagatgcc gtagccaacc gatgccacgc catccacgag ccgatggttt ttccagaaca
     1141 catacggctg gcccggaagg ctcgcgccgc caacgtccag gaagagattt gcgcgaatgc
     1201 ccgtcacggc aatgaccgga gtaaccagga cgtcgatcag cggaaagcgc agctcggcgt
     1261 ttgcgtagcc ggctttggtt ccgacgatcg atcggtactc gaagccacga acggtattca
     1321 agccgccgaa gtaaaagaag ttcgggaacg aaccgtgcga ataccctgcg aatacgcgcg
     1381 tcgcaagcaa tgcacgcgaa ctgatcggaa gatactggcg ggcatcgatc gtgtagtcgt
     1441 tggtcagcac tccgctggtg ccggcgctcc ggtggacgtt cggcgcaaac accgtattga
     1501 gttcgtagcg atgccctgca atcggtccga attccttgaa cgatgttgta tcgcccgtaa
     1561 agctggctgt cacaaacggg aagttgtcgc ggcgattgac gaagaaaaca tctccagcgt
     1621 cgtctatgaa cgggtaattg acatcacgct cttcgtagcc catgcccgca tcgactcggt
     1681 gatagcgatc gaagggatgg ctcagaattc cgacgaagcc ggtctcgcgg taaagctggc
     1741 ggcggtcgag cgtgaagtcg ctggtcgtcg aaagcgtggt gaagaacgtg cggctgtcga
     1801 aaatccggaa tccccagttg gtgcgattgt gcaggtcgag gtacagaaag tcgaaatttg
     1861 agaacgacga aacggaatcc agtgaggcga tgaagcgccg gtcgccgagc atgtcactca
     1921 tgaagatgac cgaccgcgac aggaagaggt tatcggacgt gacgccggcg ttcacttcga
     1981 cgtcatcgat gaagagtttg tggctgttca cggtttcgat cttgtccgga tcgatcgtca
     2041 cctcgaccgg cggctgaaaa aatgggcggg ccgaggccag catcggcgcc gaaggcaaca
     2101 cggtcttctc cgccgcatgc agaggcttgt cggtcgtcgt cgagtaaagg cgccagtgac
     2161 gtttgtagta agaggcaaat accatccgct cgcggttgtt cgtgccggtg aaaacaaccg
     2221 gcgtgaagca gccgccgacg acgtcggtgt attgcagcag gtcgccgttg tcgagattga
     2281 gcgaatagat gttgtaggcg gcgtatttcg tgagatcgac cggcggtgta gcgctctccg
     2341 gtttcgcctt gtcttccgcg ttctgcagaa tctccactgc cgcggcgata ttgcggccgg
     2401 tctgcttgtc ggatgcgaaa aagatccgct tcccgtcagg cgagaaccac gagtcgatgt
     2461 cgttccactc acccgtggtg agctgaaagc gctccgaagg gttcgccaga ttcagcttga
     2521 acagcttggc ataaccattg acgacggatg agtacagcag ccatttacca tcgggcgaga
     2581 aaaccggcgc gccgtcgaag aacttatcgt tcgtcaagtt tctgattgcg ccgctgccaa
     2641 tgtcataaag ataaatgtcg gggacgttcc ctttgaaccc ggcgaaggca atcgtcttgc
     2701 cgtcgggcga ataggtgggc gcgagctgct gctcgaaatc catcttcact gaacgatcga
     2761 tatggccgtt cagagcattg atgagcagca gattccggcc gcgctccttc ttcacaaaga
     2821 gagcgatctg gtcgccgttc ggcgagaagc cgacgtcgcg gcccatgacc ggcccgactg
     2881 taagcatctg cgcgatcgcg tactcgtatt tggtcgtgaa accgctcgtc agattccgaa
     2941 gcagacgccg ctcggggatg ttgtagagga tgacgtcgac atcttccttg taggtcgcga
     3001 atgccgccaa aagatcgccc gatggcgacg gcgacggcga catatcctcg ctctcggtac
     3061 cctcggaaat cttgaacgaa tcgccatact cctgcggctc accctttgac acgagagcgg
     3121 gcaggtattg cttgcgcagc caggtgcgga actgcgagtc gaactcgtcg ggcttcacat
     3181 cgaaggcgcg cttgagggcc ttgtcgacgg agttgcccag cgtgttgcga tactcgtaaa
     3241 tgaaatcgcg aagtccttcc cacccgtact tctgctgaat gaacgcaaac accgcgtgac
     3301 cgaaacggta cgcaaagtag cctccgccct gggcgtgcgt gatcggtggc accgcatcat
     3361 tgacgacggc gtcgcgcagc accatgcggt ccttgctgtc ttcgtcgttc cccatgaacg
     3421 acgccagacc ttccatcatc caagtcggcg gattcgaagt gacttccttc cccagtttgc
     3481 cttgaaacag aatctcgtac tcgaacacgt gcgtcagttc gtgctgtacg agttggagaa
     3541 gtttttcgtc ggtggtatcg atcggaatga ccatccggtt gcgcgttggc tccgcgaacg
     3601 cacccacgcc ttcgggaatg aaattcagat cgacgttcgt ctgctcgaac gccgagtgcg
     3661 tggcgtaata gatgatcggg atcttcttgg agacctggaa gttgaacttg cgcgagagat
     3721 cgtcatacgc gctctccgcc atgtcgacga ccttctccag cgccggcttt tcctcatcgt
     3781 agaaatagat gtcgaaatgc gtggcgtgat agacgttcca atggaacttg tcgtagacga
     3841 tcttgttctg gccgaacgga aacgcactcg gcgcgaacag
//
LOCUS       DATLVW010000123        14082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_541736, whole genome shotgun sequence.
ACCESSION   DATLVW010000123 DATLVW010000000
VERSION     DATLVW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14082
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541736"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1022)
                     /locus_tag="VNN25_04015"
     CDS             complement(135..1022)
                     /locus_tag="VNN25_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HXH90724.1"
                     /translation="MKRFLAIVLLFAACRYERPAATIEPGSHQEEIAAWRANRASRLR
                     APDGWLALAGLYWLSEGNNDVSLPAKPPVPSQFVLQNGKVTLNPNPALMIEGKPVTAA
                     VELKNDTDAKPTVVRTGTLSLVAIRREDAVHGPRFGVRVRDTAADARTHFLGLEYFPA
                     DASYRVEARFEPYNPPKTIPITNVLGMTSNEISPGALVFTLQGKEFRLDPILEQGEKD
                     YFVIFKDATSGKETYGAARYLYAHPPDASGKTIVDFNKAYNPPCAFTPYATCPLPPPQ
                     NRLPIRIEAGEKKYAGGHG"
     gene            1131..1697
                     /locus_tag="VNN25_04020"
     CDS             1131..1697
                     /locus_tag="VNN25_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH90725.1"
                     /translation="MIPAWNEESRLLAAVRGGDRSAAEELVDRTYSSVFASLFRLCGD
                     RELAADLTQETYRKAWEALSGFSGGSQFFTWLYRIGYTTFLNHIRRPRLMKSLDDENA
                     PELPDSAPSPEDVLSSSEESERLRNAVLTLSEDLRFTVTAHFWGGLAVKEIASIEGIT
                     TVAIRKRLHKAFTLLEEVLGAAVEEEGR"
     gene            1694..2974
                     /locus_tag="VNN25_04025"
     CDS             1694..2974
                     /locus_tag="VNN25_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90726.1"
                     /translation="MTHNRKADLQRKLAMASVATPPDGLADRIKNDIPNDLRFAPESA
                     RKQMRQSAMFNLRIAASIVLLVSSLYFALHLFSRAGSNANAVATFHEPASAPAPMRPA
                     NVTLPNIPPQPGSIHAQQAADLPTLPGAPPSSIGSVQPAMRLSEKKREEAVGMTAGTP
                     AYVDTVEAPTAAPEPMIVTNAAPSPGRVAEMEGFAPERAKSDLATTAKTSAAPSIASA
                     QAAGALAPPPPAAAPRMAEARDAAPARNFVAIEESIARGETPRNGDLDVIVRHFAAPE
                     SVPSGVRVELEASSAPLDASKRLLRVSVDAATSNGEDITLTFGDAVESHHALTGTLAP
                     NETALYEIEFKRTATPDQMIASVKVGGVTRTIRGNDLHTWNSASTRMKRASLAAAWAR
                     TLQSRSRADAIVVRAREAHIDDLADLAERVERNR"
     gene            complement(2983..3237)
                     /locus_tag="VNN25_04030"
     CDS             complement(2983..3237)
                     /locus_tag="VNN25_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90727.1"
                     /translation="MTFTKEQVSGLAKTPRCPRCDLNKTIGNALCRSCRAKLPENMRM
                     ALEKIPSKDAWIVSRAVRAAARYFDVHFQSVRNFGGGRPR"
     gene            3353..4015
                     /locus_tag="VNN25_04035"
     CDS             3353..4015
                     /locus_tag="VNN25_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXH90728.1"
                     /translation="MGERVEFPSNGHSCSGYVAGKGPGIVVIQEWWGLVPHIEDIVER
                     FAREGFSAIAPDLYHGATTSSPDEAGRMLMELDADRAEKEIAAAGDWLLNRPECTSKK
                     YGVAGFCMGGALAQYTATKESNAAATVSFYGGFKKVAFDWKNLSAPILLIYAEEDQGV
                     PASQGRDLAKQLEEMGKDVQLVVYPNVNHAFFNDTGRNYNATAAADAWKRTIEFFRAN
                     VK"
     gene            4015..4938
                     /locus_tag="VNN25_04040"
     CDS             4015..4938
                     /locus_tag="VNN25_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HXH90729.1"
                     /translation="MQTLRIVAFVAFLLFCLTPWASPPIALALGLVLALTLGHPYPAR
                     NSKITKMLLQVSVVLLGFGMNLQKVIEAGRTGILFTIVTIIGTLLLGFLLGRAMGISR
                     TTAHLISSGTAICGGSAIAAVGPVVNANDEEMSVALGTVFILNSIALFAFPMIGHRLG
                     LTQPQFGVWAAIAIHDTSSVVGAAAKYGAEALQIATTVKLTRALWIVPLTIGTAFFFK
                     HKSAKIALPWFILWFVVASVIRTYVTAPAELWPALTMLARIGLTITLFLIGAGLSRKS
                     IAAVGARPLILGVVLWIVISCASLLAVMRMV"
     gene            5094..5519
                     /locus_tag="VNN25_04045"
     CDS             5094..5519
                     /locus_tag="VNN25_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90730.1"
                     /translation="MTDQIDPMTDQNGPMTDQIGPITDQNGPITDQIGPITERNGPMT
                     ERNGPMTERNGPITERNGPMTERNGPITDQIGPITDQIGPITERNGPITEQNGPMTDQ
                     NGPMTEWAGPMSEATVPISQPSACSSHNLFSRALPPSPP"
     gene            complement(5488..6327)
                     /locus_tag="VNN25_04050"
     CDS             complement(5488..6327)
                     /locus_tag="VNN25_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90731.1"
                     /translation="MKTNVANGIPRREFIRLGSFAVLGAAVLSKAETNLLGATSESGI
                     VPLMGVGYAPSLPLQGYSNPLASANSILSPDPRFISSGARVSVIGAARAASHANAAGG
                     IAVDALFAVSHRRPDDPLRFSFFSINGRTDHDDVTGPISFMIQVPSTGGVPFVVRRLR
                     PTIQKESTEQPSLEPEVAPLTLSLGNVAGPKLTRGVYVLAFREEDSDGMSDWSRMAIA
                     RVDTGYVISGATFSYLILNVDYADEVQDRRRALRGSVTGSLQEAAIPHFSPLTVGLAA
                     ALC"
     gene            complement(6489..6944)
                     /locus_tag="VNN25_04055"
     CDS             complement(6489..6944)
                     /locus_tag="VNN25_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90732.1"
                     /translation="MSTTASDYLCEISSLLDGGYPGDCVTHACRLAELLLAERKNPWI
                     GRVRDIRQVTLGTFHGPLTPIRLLGREGPTWTTHYVACKDGYAYDPLVGRPVPLAEYP
                     VIVFGRALAIDCFLDVGTTAELCLRQELPSAFRVQRVPRQSVETSGRRA"
     gene            7206..13709
                     /locus_tag="VNN25_04060"
     CDS             7206..13709
                     /locus_tag="VNN25_04060"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH90733.1"
                     /translation="MRPSVKSRLLLLLLFVLLLVPAVPAAADVTVSGTVNFSSLDGSA
                     QDDDHTVNGVFTVNGNLTVLGTINCNDTGAGSNSACPMQFAVSGNVVLAPGSGVFAEN
                     RSSEGNGADITFIVGGNVLMQGPSSPLAGAIVSSSKLNGGNNYHAGNITFNAAGTVTQ
                     EAGSVIAATAQDSFAGAISITSGAASSIGGQILSGPTRTIATSTLYTNVIMSGGGGHY
                     VGGPISIKALTHDEPGLIITSDAIIASQGSETGPTTGSPVTLEACNVQINGLVASLAQ
                     KLSGPGVVVKSGTGITIDSSTLASGAGAHRGCVRSDATHEDAGTFYVNLFAKDAITVI
                     GPATSSTLFSVTSNPGTTDKRNAGTITVISTAGIVTASGNAFQVSGTRNGDRGGTVNV
                     SAMNGVTLSGTSIDASGATSDANRAGGHINVRSYSAGVAWLNGVGDVRPVGSTAGVPV
                     GQQGTISLTYCTTLSTSGTSFPTNGSPVGVFPTTAQTCSPAAPSLPAGVTLPDCNTPP
                     TANDDSYTVAEGGTLNVPPPGVLANDTDPDSPTLTAILVSGPAHASSFTFNADGSFTY
                     VHNGGETTSDSFTYKANDGSLDSNVATVHITITPVNDAPIANNDTATVAEGGTLNVAA
                     PGVLANDTDPDSPTLTAILVSGPAHASSFTLNSNGSYVYVHDGSETTSDSFTYKANDG
                     SLDSNVATVSITITPVNDAPTANNDSYSVNEGGTLTVAAPGVLGNDTDPDSPTLTAVM
                     VSGPAHASSFTLNANGSFTYIHDGSETTSDSFTYRANDGSLNSNIATVSITVNPVNDP
                     PVAANDGPYSVLEGGTLNVAAPGVLGNDTDPDSPTLTAVLVSGPAHAASFTLNADGSF
                     TYIHDGSETTSDSFTYKASDGSLFSNVATVSIAVTPVNDAPVANNDSYGVNEGGTLTI
                     AAPGVLSNDTDPDSPTLTAVLVTGPAHAASFTLNADGSFSYVHDGSETTSDSFTYKAS
                     DGSLFSNVATVSITITPVNDPPVANDDTYAVNEGGTLNMAAPGVLANDTDPDSPTLTA
                     ILVSGPAHAASFALNADGSFSYVHDGSETTSDSFTYKANDGSLDSNVATVTITIAPVN
                     DAPVVTAGGTLTFTEGDPATVIDNSITISDSDSPNLASATVTISGNYANGQDVLAFTN
                     TANITGSFNAGTGTLTLTGSDTVANYQSALRSVTYSNSSVNPSTLQRTVTWQVNDGAG
                     VNNLSAPVTSTINVISVNSAPQLANSSTITYTENDPATVINSVITVSDVDSPNLIGAT
                     VQITTGYNSAQDVLSFAPAFGITGSFNSGTGTLTLSGTSSVANYQAALRSVKYTNTSD
                     DPTTAARTVVFQVDDGGATNNLSNTVSSTINVTAVNDPPTAFAFSGLPAQAGIPITYP
                     AGKLGGSDVEAGTTITIDTTPINVVNGSVVINANGSFTFTPIPQSAGGTASFQYRVSD
                     NGNPAPGVNGAYVTVSFSVAGPAIYFVKALGAGNCTLGNECTLTTALANIGASSNADI
                     FLGDASTHSAGAFALNSGGSIIGQGVVAASFDALFGIGTPSQGVLAPRPAINQPRPTL
                     VNSPLTLNNGSSARGFNMSNTTGDTLFALSKTGLTVTDTNITTTSNSSGQFGVNIVTS
                     SGTFAFGTITVTGGNSGSGVNFNNTTSASLVSFGDISTNGGNAVTIASSGATNFSFAN
                     VSSTTGKAVGITTATGAFSFTKISSNGAPKGITVSGLTGAGSFTVNGTGGLCDSTHIG
                     ASDCTGGTIQGSSLRGAEFTNAANVTLKNIYFKGDGTTNSGATCIDNVASGSNVTCNA
                     ALYLSGASATTLDRLYLDGTGSADMGLNGNAVSGLTMSNTEIANFTGSTKTAMLFQNL
                     TGTVGVTAANIHNNNVTHNVFVTNNTGTANITFTNPIVQTSSGAGNPDGIQVDSFVSG
                     TTLNVTASGVTISNVPGNGVTFQASSGSTMTGVLNGGTVTGTNGILMQSAGNNTSLTY
                     TVTGLTAVTTHNFGSNAITAGKANGTNGTVNGTITNNTITSALCGGGCNGITIASFGT
                     SGTSVVNVSGNDISGVDASAIAATAGQGSSSLNLTVQGNNIHNPDPGNSSYAIDLIAG
                     TSAGDAPCFFGNIGDMSSTHTVPANRNVITGSWQSGGNPIEAGIFNNSSFKLLNLVGS
                     GDTAAALWLTASNTALGADTFHLGPNQFINGTSCP"
     gene            13784..>14082
                     /locus_tag="VNN25_04065"
     CDS             13784..>14082
                     /locus_tag="VNN25_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90734.1"
                     /translation="MVRNETLRRIVDFIRSIGIEVDEGAMTRETLVPGIDIVNGSLLI
                     DEPKMCKPADLLHEAAHIALTRSDARSALDGTVNTSAAEEMSAIGWTWAASRHLQ"
BASE COUNT         2904 a         4578 c         4149 g         2451 t
ORIGIN      
        1 cggcatgcgt ggcgcaggtg attgcggacg agcgaaacgc gaagtaggcg gcgcgcggtt
       61 gcgtagcaat taccgcatta cgaaagtcgc agcgacgccg ccaaacttcg agaccgcgta
      121 accccgcgcg accgttaccc gtgcccgccc gcgtacttct tctcccctgc ttcgatccgg
      181 atcggtagcc ggttttgcgg aggcggcagc ggacaggtcg cgtagggcgt gaacgcgcaa
      241 ggcgggttgt acgccttgtt gaaatcgacg atcgtcttcc cgctcgcatc ggggggatgc
      301 gcgtagaggt agcgcgcagc gccgtaggtt tcctttccgc tcgttgcatc cttgaagatg
      361 acgaagtagt ccttctcgcc ctgctcgaga atcggatcga gtcggaactc ttttccctgc
      421 agcgtgaaga caagggcgcc gggggagatc tcgttgctgg tcatgccgag gacgttcgtg
      481 atcgggatcg tcttgggcgg gttgtagggc tcgaagcgcg cttccactcg atagctcgca
      541 tcggcgggaa agtactcgag gccgagaaaa tgcgttcgcg cgtccgcagc ggtatcgcgc
      601 actcgaactc cgaatcgtgg cccgtgcacg gcgtcttcgc ggcggattgc gaccagcgac
      661 aacgtccctg tacgaacaac ggtcggcttc gcgtctgtat cgttcttgag ctcgaccgcc
      721 gccgtcaccg gcttcccttc gatcatgagc gcaggattcg ggttcagagt caccttgccg
      781 ttctggagaa cgaactgcga cggaacaggc ggcttcgcag ggagggagac gtcgttgtta
      841 ccttcgctga gccaatagag accagcgagc gccagccagc cgtcgggagc gcgaaggcgc
      901 gaggctcgat tggcacgcca cgctgcgatc tcttcctggt gcgatccggg ctcgatcgta
      961 gcggcgggac gctcgtagcg gcaagcagcg aatagaagaa cgatagcgag gaaacgtttc
     1021 attggtcgga taggatactc aactcgtggt ttcgaaatgt cgcagagccg tgtccaatcg
     1081 gggtgagcaa tgttgcgcct tttcccgcca tccggccgga gcctgactcg gtgatccccg
     1141 cctggaatga ggagagccgg ttactggcgg cggttcgagg cggcgatcgc agcgccgcgg
     1201 aagagctcgt cgaccgaacc tactcatcag tcttcgcctc gctctttcgt ttgtgtggcg
     1261 atcgcgaact ggccgctgat ttgacgcagg aaacgtaccg caaggcttgg gaagcgctgt
     1321 ccggtttcag cggcggctcg cagtttttca cgtggttgta ccgcatcggc tatacgacgt
     1381 ttctcaatca catacgccgg ccgcggttga tgaaatcgct cgatgatgaa aacgcaccgg
     1441 agctgcctga tagcgctccg tcgcctgaag acgtactgag cagttccgaa gagagcgaac
     1501 ggcttcgcaa cgcagtgctg acgctatccg aagatctccg tttcaccgtg acggcgcatt
     1561 tctggggcgg ccttgcggtg aaggagattg catccatcga aggaatcacc accgttgcca
     1621 ttcgcaagcg gctgcacaag gccttcaccc tcctggaaga agtcctcggc gcggctgttg
     1681 aggaagaagg acgatgacac acaaccggaa agctgatctg cagcggaagc tggccatggc
     1741 ctcggtggcg acaccgcccg atggtctcgc cgaccgcatc aagaacgata tcccgaatga
     1801 tctccgattc gctccggaga gcgcgcgcaa acagatgcgg cagtcggcaa tgttcaacct
     1861 tcgaatcgcc gcgtcgatcg tgttgctcgt ctcttcgctc tattttgctc tgcacctgtt
     1921 ttcgcgcgcc ggatcgaatg cgaacgccgt tgcaacgttc catgagcccg cctctgcacc
     1981 cgcaccgatg cgtccggcaa acgtgacgct gccgaacatt ccgccgcagc ccggaagcat
     2041 ccatgcgcaa caagcagcgg atctgccaac gctgccgggg gctcctccga gcagtattgg
     2101 ctcggtgcaa ccggcgatga gactttcgga gaagaaacgc gaagaagcgg tcgggatgac
     2161 cgctggtact cctgcgtatg tcgacaccgt ggaagcgcct accgccgcac cggaaccgat
     2221 gattgtgacg aacgcagcgc cgtcgccagg aagagttgcg gagatggaag gcttcgctcc
     2281 tgagcgggcc aagagcgacc tcgctaccac cgccaagact tcggcggcgc cgtccatcgc
     2341 atccgcacag gcagccggag ctctcgcgcc tcctcctccg gccgcagcgc ctcgaatggc
     2401 cgaggcacgt gacgcggcgc cggcaagaaa cttcgttgcg atcgaagaat ccatcgcccg
     2461 aggagaaacg ccgcgcaatg gagatctcga tgtgatcgtc cgtcacttcg ccgcgccgga
     2521 gagtgttccc tccggagttc gcgtcgagct cgaagcgtcg tcagcgccgc tcgatgcgtc
     2581 gaagagactt cttcgtgtgt cggtcgatgc agccacatcc aacggtgagg acatcactct
     2641 cacgtttggc gacgcggtcg aatcgcatca cgcgttgacc ggaacgttgg ctccgaatga
     2701 gaccgcgttg tatgagatcg aattcaagcg gaccgcgact cccgatcaga tgatcgcgag
     2761 cgtgaaggtt ggcggagtaa cgcgcaccat tcgcggcaac gacctccaca cgtggaattc
     2821 tgcctcgaca cggatgaagc gcgcctcgct ggccgcagcc tgggcgcgca cgctgcagtc
     2881 acgcagccgg gcggacgcga tcgtggtgcg ggcgcgtgaa gcgcacatcg atgatctggc
     2941 ggacctggca gaacgggtcg agcggaaccg gtagccccgt cgtcagcgcg gccgtccgcc
     3001 gccgaaattg cgcaccgact ggaagtgcac gtcgaaatag cgggccgccg cgcgaaccgc
     3061 acgggagacg atccaggcat ccttcgacgg gatcttctcc agcgccatcc gcatgttctc
     3121 cggcaacttg gccctgcaac tgcggcagag cgcgttgccg atcgtcttgt tcagatcaca
     3181 ccgcggacag cgcggtgttt tggcgaggcc ggaaacttgc tccttcgtaa acgtcatcga
     3241 aaccctctct tacatcgtta ttgttacatg aatacgaaac agatgcagct cgggtcacca
     3301 gattgtcacg ggtgcattaa aatccgcgcc tgttttccag gaggagtccc cgatgggcga
     3361 acgcgtcgag tttccatcca acggccattc atgcagcggc tatgtcgcag gtaaaggtcc
     3421 gggcatcgtc gtcattcagg agtggtgggg cctcgtgccg cacatcgaag acatcgtcga
     3481 gcgcttcgcg cgcgaaggct tttccgcgat cgcccctgac ctctaccacg gcgcgactac
     3541 aagttcaccc gacgaagcgg gaaggatgct gatggagctc gacgccgacc gggccgagaa
     3601 ggagatcgcc gctgccggag attggctact caaccgtccg gaatgcacgt cgaagaaata
     3661 tggtgtcgcc ggcttctgca tgggaggcgc cctcgctcaa tacacggcga cgaaagaaag
     3721 caacgccgcc gcgacggtca gtttctatgg cggcttcaag aaggtcgcct tcgactggaa
     3781 gaatctgagc gctccgattc tgctgatcta tgcggaagag gatcaggggg tgccggcctc
     3841 acagggacgt gatctcgcca agcagctcga agagatgggc aaggacgttc agctcgtcgt
     3901 ctacccgaac gtcaatcacg cgttcttcaa cgacaccggc cgcaactaca acgccacggc
     3961 tgctgctgat gcctggaagc ggacgatcga attcttccgc gccaacgtga agtagtgcag
     4021 acgctccgca tcgttgcgtt tgtcgcgttc ctgctcttct gtctcacgcc gtgggcctcg
     4081 ccgccgatcg cgctcgcgct gggtctcgtc ctggcgttga cgctgggaca cccgtatccg
     4141 gcccgcaatt cgaaaatcac gaagatgctg ctacaggtct ccgtcgtcct gctcggcttc
     4201 ggcatgaacc tgcagaaagt gatcgaggcc ggccggaccg gaatcctctt cacaatcgtg
     4261 acgatcatcg gaacgctgct ccttggattc ctgctcggcc gcgcgatggg catcagccgc
     4321 accaccgcgc atctcatctc ctcgggtacc gcgatctgcg gaggaagcgc catcgcggcc
     4381 gtcgggccgg ttgtcaacgc caacgatgag gagatgtcgg tcgcgctcgg gacagtcttc
     4441 atcctcaact cgattgcgct cttcgccttt ccgatgatcg gtcaccgcct cgggttgacg
     4501 cagccgcagt tcggcgtctg ggcggcaatc gccattcacg atacgagctc agtcgtcggc
     4561 gccgccgcaa aatatggcgc ggaggcgctg cagatcgcga cgacggtgaa attgacgcga
     4621 gccctctgga tcgtccccct gacgatcggt accgcgttct tcttcaagca caaatcggcg
     4681 aaaatcgctc tcccctggtt catcctctgg ttcgtcgtcg cctcggtgat ccgaacctac
     4741 gtgactgctc ctgctgaact gtggccggcg ctgacgatgc tcgctcgtat aggcctgacg
     4801 atcacgctct tcctgatcgg cgcgggtctc tcacgcaagt ccattgccgc cgtcggagcg
     4861 cggccactga tcctcggcgt cgttctttgg atcgtgatct cgtgcgcttc gctgctcgcc
     4921 gtgatgcgga tggtgtgaaa agttcgaaag gtgaccgcgt gcatgatgca aggtcatcga
     4981 acccgcattt ctcacactca ttagattgat tcctttccaa tcgagcgcgc caagcgtgag
     5041 aaaggcgtca aaagaacgaa aggggcggta ataagtcgga atgttttgtt ccgatgacgg
     5101 atcaaatcga tccgatgacg gatcaaaacg gaccgatgac ggatcaaatc ggaccgatca
     5161 cggatcaaaa cggaccgatc acggatcaaa tcggaccgat cacggaacga aacggaccga
     5221 tgacggaacg aaacggaccg atgacggaac gaaacggacc gatcacggaa cgaaacggac
     5281 cgatgacgga acgaaacgga ccgatcacgg atcaaatcgg accgatcacg gatcaaatcg
     5341 gaccgatcac ggaacgaaac ggaccgatca cggaacagaa cggaccgatg acggatcaaa
     5401 acggaccgat gacggaatgg gcgggaccga tgtcggaagc gaccgttccg atatcgcaac
     5461 cgtcggcttg cagttctcac aaccttttca gcagagcgct gccgccaagc ccaccgtaag
     5521 tggtgagaaa tgcgggatcg cggcctcttg aaggcttcca gtaacgctac cgcgcagcgc
     5581 gcggcggcga tcctgaacct cgtccgcata atcgacgttc agaatcaagt acgagaacgt
     5641 ggcgccggaa atgacgtagc cggtatcgac tcgcgcgatg gccattcggc tccagtcgct
     5701 catcccatct gagtcttcct cgcgaaacgc cagcacgtag acgccacgcg tgagcttcgg
     5761 cccggcgaca ttgccgaggg aaagggtcag cggcgccact tcgggctcga gcgacggctg
     5821 ctctgtcgat tccttctgaa tcgtcggccg caagcgtcgc acaacgaagg gcacaccgcc
     5881 tgtggagggt acctgaatca tgaaagagat cggaccggtc acgtcgtcat gatcggtacg
     5941 gccgttgatc gagaagaagc tgaaacgcag ggggtcgtcc ggcctgcgat gggaaaccgc
     6001 aaaaagagcg tcgacggcga tgccgccggc ggcgttcgca tggcttgccg cgcgtgccgc
     6061 gccgatgacg gaaacccggg cgccgctgct gatgaatcga ggatcggggc tcagaatcga
     6121 gttggcactg gccagaggat tcgaataccc ctgcaacgga agtgatggcg cgtaaccgac
     6181 tcccatcaga ggcacgatgc cggactcgct cgtggccccg agaaggttcg tttcggcctt
     6241 cgacaagacc gcggcaccaa gaacagcgaa tgatccgagg cgaatgaact cgcggcgtgg
     6301 aatcccgttg gcgacgttcg ttttcataca gctctccatg cggcatgcgt tcggaagttg
     6361 cgcagtgcta ggaacgtgag aacgattggg gcgacgaaaa gatactagca cggccgcgcc
     6421 aactcaagag gaaagcgagc ggctcctcat cgcaagaccc caaacgtcga tctcgtgctg
     6481 cgcgacactc aggctcgtcg accactggtc tcgaccgatt gccgcgggac ccgctggaca
     6541 cgaaacgcgg acggcagttc ctgccgaagg cagagctccg cggtggtgcc cacatcgaga
     6601 aaacagtcaa tcgccaacgc ccgcccgaag acgatgaccg gatactccgc gagaggaacc
     6661 ggtcttccga caagtgggtc gtacgcatag ccgtccttgc atgcgacgta gtgcgttgtc
     6721 catgtgggcc cctcgcgtcc caatagtcga atcggtgtta ggggaccgtg gaaggttccg
     6781 agcgtgacct ggcgaatgtc gcgcacgcgg ccgatccagg gattctttcg ctcggcgagc
     6841 agaagctctg ccaaccggca cgcgtgcgtg acgcaatctc ccggataccc gccatcgagc
     6901 agggatgaaa tctcgcagag gtagtccgaa gcggtagtgc tcaacgaaac gattctaacg
     6961 agatccggat ggaagaccag ccgcgcccca acgcctaccc catccgaagc tgcctcggtg
     7021 gttacttacc aaatgtggtt ggtctggcca tagccatcga atcgatccgg cccagctttc
     7081 agtgccttcc gtaaacatgg agcagaaagg agccacttct tccattattg gcgttatgat
     7141 tcgcgctcac aacaagctca acggtcgtcc acttacttca gcgggatatc gaaaggacat
     7201 ttctgatgcg tccaagcgta aagtcgcgtt tgctccttct cctcctcttc gttctgcttc
     7261 tggttccggc tgtcccggcc gcggcggacg tcaccgtcag cggcaccgtc aacttctcgt
     7321 cgctcgacgg ctcagcgcaa gacgacgatc acactgttaa tggtgtgttc accgtcaacg
     7381 gcaacctgac cgtgctcggc acgatcaact gcaacgacac aggcgccgga agcaactccg
     7441 cctgtccgat gcagttcgcc gtgagcggta atgtcgtgct cgcgccggga agcggagtct
     7501 tcgccgagaa caggtcgagc gagggcaacg gcgcggacat cacgttcatc gttggcggca
     7561 acgtgctgat gcaggggccc agcagcccgc tcgcgggagc aatcgtttcg tcgtcgaagc
     7621 tgaacggtgg gaacaactac cacgccggca acatcacgtt taacgccgcc ggcacagtca
     7681 ctcaagaggc aggctcggtc atcgccgcga ctgcacagga cagtttcgcc ggcgcgatct
     7741 cgatcaccag cggagccgcg agctcgatcg gcggccagat cctctccggg ccgacgcgga
     7801 cgatcgccac gtccacgctg tacacgaacg tcatcatgag cggcggcggc ggccactatg
     7861 tcggcggccc catctcgatc aaagctttga ctcacgacga gccgggcctg atcataacca
     7921 gcgacgcgat catcgcttcg cagggctcgg agactggacc gactacggga agtccggtca
     7981 ccctcgaagc ctgcaacgtg cagatcaacg gactcgtcgc gtctctggcc cagaagcttt
     8041 cgggtccggg cgtggtggtc aagagcggaa ccggcatcac gatcgacagc agcaccctgg
     8101 cttccggcgc tggcgcgcat cgcggctgcg tgcgatcgga tgcgacgcac gaggacgctg
     8161 gtaccttcta cgtcaatcta ttcgcgaagg acgccattac cgtcatcggt ccggccacgt
     8221 catcgacgct attctccgta acgtcgaatc ccggcacgac ggacaagcgc aacgccggca
     8281 cgatcaccgt catctccaca gccggaatcg tcacggcgag cgggaacgca tttcaggtct
     8341 cgggtacccg caacggtgac cgcggcggta ccgtcaacgt ctcggctatg aacggcgtca
     8401 ccctgagtgg caccagcatt gatgcgtcgg gtgcgacgag cgacgccaac cgcgccggcg
     8461 gtcacatcaa cgtccgctcg tactcggccg gagtggcgtg gctgaacggc gtcggcgacg
     8521 tgcgaccggt tggaagcact gccggcgttc ccgttgggca gcagggaacg atctcgctca
     8581 cgtactgcac gacgctgtcg acttcgggta cgtccttccc gacgaacggc agccccgtcg
     8641 gcgtcttccc aacgaccgcg cagacgtgct cccccgccgc gccgtcgctt ccggccggcg
     8701 tgacccttcc tgattgcaat acccctccga cagccaacga cgattcctac acggttgcgg
     8761 aaggtggcac gctgaatgtt ccccctcccg gcgtactggc caacgatacc gatcccgatt
     8821 cgccgacgct cacggccatc ctcgtgagcg gtcccgccca cgcgtcatcc ttcaccttca
     8881 acgccgatgg ttcgttcacg tacgtgcaca acggcggcga aacgacgagc gactcgttca
     8941 cgtacaaggc aaacgacggt tctctcgact cgaacgtggc cacggtccac atcaccatca
     9001 cgccggtgaa tgatgcgccg atcgcgaaca acgacaccgc cacggtcgcc gagggcggga
     9061 cgctcaacgt ggcagctccg ggcgtactgg cgaatgacac cgatcccgac tcgcctaccc
     9121 tcactgcgat cctcgtgagc gggccggcac acgcctcctc gttcacgctg aattcgaacg
     9181 gctcgtatgt ctacgtgcac gacggcagcg agacgacctc cgatagcttc acttacaagg
     9241 ccaacgacgg ctccctcgac tcgaacgtcg ccacggtcag catcaccatc acgccggtca
     9301 acgacgcgcc gacagcgaac aacgacagct actcggtcaa cgagggtggc acgttgactg
     9361 tcgccgctcc gggcgtcctc ggcaacgaca ccgaccctga ctcgccgact ctcaccgcgg
     9421 tcatggtgag cggcccggcg cacgcgtcgt cattcacgct caatgcgaac ggctcgttca
     9481 cgtacatcca cgacggcagc gagacgacgt cggacagctt cacctatcgc gcgaacgacg
     9541 gctcgctgaa ctcgaacatc gccaccgtat ccatcacggt caatcctgtg aatgacccgc
     9601 ctgtggcggc caacgacgga ccgtacagcg tcctggaagg cggcacgctg aacgtcgccg
     9661 cgccaggcgt gctcggcaac gacaccgatc cggacagccc aacgctgacc gccgttctcg
     9721 tcagcggacc ggcgcacgca gcatcgttca cgctcaacgc ggatggctcg ttcacgtaca
     9781 tccacgatgg cagcgagacg acgtcagaca gcttcacgta caaggccagc gacggctcgc
     9841 tcttctcgaa cgtcgccacc gtttcgatcg cggtcactcc tgtcaacgat gcgccggtcg
     9901 cgaacaacga ctcctacggc gtcaacgaag gcggcacgct gacgatcgcc gcgcccggcg
     9961 tcctgagcaa cgacaccgat ccggacagcc caacgctgac tgccgttctc gtcaccggac
    10021 cggcgcacgc cgcatcgttc acgctcaatg cggacggctc gttcagctat gtccacgacg
    10081 gcagcgagac gacctccgac agtttcacct acaaggccag cgacggctca ctcttctcga
    10141 acgtcgccac cgtttcgatc accatcactc ccgtcaacga cccgccggtc gcgaacgatg
    10201 acacctatgc ggtgaacgaa ggcggcacgc tgaacatggc tgcgccgggc gttctggcca
    10261 acgacacgga tcctgacagc ccgacgctga ccgcgatcct ggtcagcggt cctgctcatg
    10321 ccgccagctt cgcactgaac gccgacggtt cattcagtta cgtgcacgac ggaagcgaga
    10381 cgacctccga cagcttcacc tacaaggcca acgacggctc gctcgactcg aacgtggcca
    10441 cagtcacgat caccatcgcg ccggtaaacg acgcgccggt ggtaacggcg ggcggaacgc
    10501 tgacgttcac cgagggcgat ccggctacgg tcatcgacaa ctcgatcacg atcagcgaca
    10561 gcgattcgcc gaacctcgcc agcgcaacgg taacgatcag cggtaactac gcgaacggac
    10621 aggatgtact ggcgttcacg aacacggcga acatcaccgg ttcgttcaat gccggaaccg
    10681 gcacgctgac gctgaccggt tccgacaccg tggccaacta tcagtccgcc ctgcgctcgg
    10741 tgacctacag caactcctcc gtgaatccat ccacgctgca gcggacggta acctggcaag
    10801 tcaatgacgg cgccggcgtg aacaacctca gcgctcccgt gacgtcgacc atcaatgtga
    10861 tctccgtcaa cagcgcaccg cagctcgcca acagctcgac catcacctac acggagaatg
    10921 atccggccac cgtcatcaac tcggtcatca cggtcagcga cgtcgattcg ccgaacctga
    10981 ttggcgcgac cgtccagatc actaccggct acaacagcgc gcaggacgtc ctctcgttcg
    11041 cgcctgcgtt cggcatcacc ggcagcttca actccggcac cggcacgctg actctgagcg
    11101 gcacatcctc ggtcgcgaat taccaggccg ccctgcgcag cgtcaagtac accaatacct
    11161 cggacgatcc gaccaccgcc gcgcgcacgg tggtcttcca ggtcgacgat ggcggagcca
    11221 ccaacaacct gagcaacacc gttagcagca cgatcaacgt cacggccgtc aacgatccgc
    11281 cgaccgcatt cgcgttcagc ggtctaccgg cacaggcagg catccccatc acgtatccgg
    11341 ccggcaaact tggcggctcc gacgtcgaag ccggaacgac gatcaccatc gacaccaccc
    11401 cgatcaacgt cgtcaacggc tcggtcgtga tcaatgcgaa cggaagcttc acctttacgc
    11461 cgattccgca gtctgccggc ggcacggcat cgttccagta ccgcgtctcc gacaacggca
    11521 atccggcgcc tggtgtcaac ggcgcgtacg tgaccgtgtc attcagcgtt gccggtccgg
    11581 ccatctattt cgtgaaggcc ctcggagcgg gcaactgcac gctcggcaat gagtgcacgc
    11641 tgacgaccgc tctcgccaac atcggcgcgt cgtcgaacgc cgacatcttc ctcggcgatg
    11701 cgagcacgca ctcggcgggc gcattcgctc tgaactcggg cgggtcgatc atcggacagg
    11761 gagtggtcgc ggccagcttc gacgctctct tcggcatcgg cactccgtct cagggcgtcc
    11821 tggctccccg gccggcgatc aatcagccgc gtccgaccct ggtcaactct ccgcttacgc
    11881 tgaacaacgg ttcttcggcg cgcggcttca acatgagcaa caccacaggc gacacgctct
    11941 tcgcgttgtc gaagaccggc ctcacagtca ccgacaccaa catcacgacc acatcgaact
    12001 ccagcggtca gttcggagtc aacattgtca cgtcgagcgg aacattcgcg ttcggcacga
    12061 tcacggtgac cggcgggaac tccggtagcg gtgtgaactt caacaacacc acgagcgcat
    12121 cattggtgag cttcggcgat atctccacca atggcggcaa cgctgtcacg atcgcaagca
    12181 gcggcgctac gaacttctcg ttcgctaacg tctcgtcgac gaccgggaag gcggtcggca
    12241 tcaccaccgc cacgggggcc ttcagcttca cgaagatcag ctccaacggc gcaccaaaag
    12301 gcatcacggt cagcggtttg accggcgcgg gcagcttcac ggtgaacggc acgggcggcc
    12361 tctgcgactc gacccatatc ggcgccagcg actgcacggg cggcacgatc cagggaagct
    12421 cactgcgcgg agccgagttc acgaatgccg cgaacgtcac gctgaagaac atctacttca
    12481 agggcgacgg aaccacgaac tccggtgcga cctgcatcga caacgtcgcc tcgggctcga
    12541 acgtcacctg caacgccgcc ctctacctca gcggcgcctc ggccacgacg ctcgaccggc
    12601 tctacctcga cggaaccggc tcggcggata tgggtctcaa cggcaacgcc gtcagcggtt
    12661 tgacgatgag caacaccgag atcgcgaact tcactggcag cacaaagacc gccatgctct
    12721 tccaaaacct cacgggaacg gtgggcgtga ccgcggcgaa catccacaac aacaacgtga
    12781 ctcacaacgt cttcgtcaca aacaacacgg gaacggccaa catcaccttc accaacccga
    12841 tcgtccagac gtcgtccgga gcgggcaacc ctgacgggat ccaggtcgac tcgttcgtct
    12901 caggcacgac actgaacgtc acagcgagcg gagtcaccat cagcaacgtg ccaggcaacg
    12961 gcgtcacgtt ccaggcgagc agcggctcga cgatgacggg cgtactcaat ggcggcaccg
    13021 taacgggtac gaacggcatc ctcatgcagt cggctggcaa caacacgtcg ctcacctaca
    13081 cggtcaccgg cttgacggcg gtcacgaccc acaacttcgg atcgaatgcc atcacggccg
    13141 gcaaggccaa cggcaccaac ggcacggtca atggcaccat cacgaacaac acgatcacca
    13201 gcgcgctgtg cggcggtggt tgcaacggca tcacaatcgc gtcgtttggc acgagtggca
    13261 catccgtcgt caacgtctcg gggaacgaca tctccggcgt cgatgcttct gcgatcgcgg
    13321 caaccgcggg tcagggtagc agctcgctga acctgacagt tcaaggcaac aacatccata
    13381 atccggaccc gggcaactcc tcatatgcga tcgacctcat cgccggcacg tcggctggcg
    13441 acgccccatg cttcttcggg aacatcggtg acatgagctc cacgcacact gttccggcaa
    13501 accgcaacgt gatcaccggt tcgtggcaga gtggcgggaa ccccatcgaa gccgggatct
    13561 tcaacaacag ctcgttcaag ctgctgaacc tcgtggggag cggagatact gccgcggccc
    13621 tgtggctcac agccagcaac accgcactcg gcgccgatac gttccacctc ggaccgaacc
    13681 agttcatcaa cggcacgagc tgcccgtagt cctcttcgga tgcgggcctc accggaggcc
    13741 cgcatccagc ttcggcccga acggcatctg agacctttca tccttggttc gtaacgagac
    13801 tctgcggcgt atcgtcgatt tcatccgttc gatcggaatc gaggttgatg agggggcgat
    13861 gacgcgggag acgctggtgc ccggcatcga catcgtcaat ggctcccttc tgatcgacga
    13921 gccaaagatg tgcaagccgg cagaccttct ccacgaagcg gcccatatcg cgctgactcg
    13981 gtctgacgcc aggagcgcac tcgacggaac cgtgaacacc tccgccgctg aggagatgtc
    14041 agccatcggc tggacatggg cggcgtcgag acatctacag at
//
LOCUS       DATLVW010000124         4789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_500778, whole genome shotgun sequence.
ACCESSION   DATLVW010000124 DATLVW010000000
VERSION     DATLVW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500778"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..90
                     /locus_tag="VNN25_04070"
     tRNA            4..90
                     /locus_tag="VNN25_04070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Leu,seq:caa)
     gene            193..2670
                     /locus_tag="VNN25_04075"
     CDS             193..2670
                     /locus_tag="VNN25_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH90735.1"
                     /translation="MRRHLIASLVLLILPIAVYALPRDGGTVRRHYILESTVPLDAAA
                     SAELAAQGIEVQKPLANHRYLVRMSDDVTPSDQRIRSLTVYDASRKIARAAYAEAAKG
                     NTSRLRILFQPDVTFDEARNTIAAAGGTIDTPFALPSVGPQHLTVRIPSMSVTSLAND
                     DRVFGIYGPPLVPKSLNAVAASISKVTPLFSAPYNLTGTGVVLSIFEPDGFPDVTHVE
                     FGGRVTSHFPAGSSVDKHATHTSGTLVAAGINAGAKGMAPAATLHAFTANPALPNGDF
                     DEDKLFQTKRDDTKAVGSVADSNSWDFAFSWQGGNVWFGNEDGYGAYSGLESEPYDAV
                     MRTVGEPLIVHAAGNDATSGNPNLTLPWAPHKHIEDQDPKTGAYPHTYCYSQNGTGTD
                     CTPALFGCSTGTIYCETKKHPTHGADTTVGLMASTKNSVTVGALNPDGVSIASFSSRG
                     PTTDGRVKPELVAKGVSQFSTVPANGYDTLDGTSMATPVVAGMSALLTQQYRKTFGKT
                     PSASILKTLLIAGADDLGPAGPDYTYGFGLADAKASVDLIIADGGTGSRIRTGTIMNR
                     QDVDIPVSLPATSKFRVVLGWFDPEVLLVPASPDDDPLADKTLINDLDVRVIDPTGAT
                     VFPYVLNGATPSALATKAANHTDTTEEVEIANAAAGTYHVLVHGAIGDPTGAAQDYVV
                     VVNAGTALAPCTDAYEPNDTQDTAFGNLMSGQTISAKICNPLDVDYFKFTSNSLSPLS
                     VSVTATDTPLKVTLSSAFSTTVTTTIAAGGSVNLGSVISTPISPTPNVPFFVRVEPNG
                     AVGATGAYTLTPTFGYNSIPRKRGAKH"
     gene            2790..3563
                     /locus_tag="VNN25_04080"
     CDS             2790..3563
                     /locus_tag="VNN25_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXH90736.1"
                     /translation="MTYKTLLVEEADGVLTVTLNRPDVHNAFNDELIAEAIDLFSGIG
                     ATGARVVVLRGTGPNFCAGADLNWMSRMVSYTREENVRDSSKLAQMYALMNECPLPVI
                     GRIQGAAIGGGVGLVAVCDIAIAARDAKFGLSEVKLGILPAVISPYVIAKIGETHARA
                     LFLTGERFDAERAQRIGLVHRIVDDVDAAIAETIAMLKTSGPEAVRECKKLIAHVASH
                     PLIDAIPYTIDAIAARRVSEEGQGGMTAFLKKEKAPWVR"
     gene            3954..>4789
                     /locus_tag="VNN25_04085"
     CDS             3954..>4789
                     /locus_tag="VNN25_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXH90737.1"
                     /translation="MIRRLLVPNRGEIAVRIARACREAGVESVLAYSELDDVRYVRRY
                     FDDAISLGNGDARETYLNVGRVIDSARRCGADALHPGYGFLSERAELAAACDEAGIIF
                     VGPKASSIAAMGSKAASRHLMQRLGVPVVPGYDGDDQNVSTLTTEASRIGFPLIVKAS
                     AGGGGKGMKIVRSAAELQSAIESAQREAKKSFGDDRLLLERYIEEPRHIEFQIFGDGE
                     GNVVHLFERDCSIQRRHQKVIEETPAPRYSEELRARMAAAAVAAARGVDYRSAGTVEF
                     IVT"
BASE COUNT          912 a         1517 c         1472 g          888 t
ORIGIN      
        1 ctggcccgag tggcgaaatg gcagacgcag cagactcaaa atctgccgag gttaaccctc
       61 atgtcggttc gagtccgacc tcgggcacca attgatgtga acagaccgtg acgccttgcc
      121 ttgtggcaat ttcgtcgcga cgctcgcgag tttcgcggcg tagagcagtc aacggagact
      181 aataactcgc gcatgcgccg tcacctgatc gcatcactcg tccttctcat ccttccgatt
      241 gctgtctacg cactgcctcg cgatggcgga accgtacgcc gccactacat tctggaaagc
      301 accgttccgc tcgatgcggc tgccagcgcc gagctcgccg cgcaggggat cgaagtccag
      361 aagcctctgg cgaatcatcg ctatctcgtg cggatgagcg atgacgttac gccgagcgat
      421 caacggatac gctcgcttac cgtctacgat gcgtcccgaa agatcgcgcg cgccgcgtac
      481 gctgaggctg caaaagggaa cacctcgcgg ctgcgaatcc tctttcaacc agacgtgacc
      541 ttcgacgaag cgcggaacac gattgccgca gcaggcggca cgatcgacac gccgttcgcg
      601 ctcccttccg tgggcccgca gcacctgact gtgagaatcc cgtccatgtc ggtgacgtct
      661 ctggccaacg acgatcgcgt cttcggcatc tatggaccac cactggtgcc gaagagtctc
      721 aatgcagtcg cggccagcat ttcgaaagtc actccgcttt tcagtgcgcc ctataacctg
      781 accggcactg gcgtggtcct ctcgatcttc gagccggacg gttttcctga cgtgacccat
      841 gttgagtttg gcggccgcgt gaccagtcat tttccggcgg gcagttccgt cgacaagcac
      901 gcaacacaca catccggaac cctggtggcc gctgggatca acgcgggtgc gaaagggatg
      961 gcgcctgccg caacgctcca tgcgttcact gccaaccccg cgctgcccaa cggcgatttc
     1021 gacgaagaca agctgttcca gacgaagcgc gacgacacga aagccgtcgg cagcgttgct
     1081 gatagcaact cctgggactt cgcgttcagt tggcaggggg gcaacgtgtg gttcggaaac
     1141 gaagacggct acggggccta cagcgggctg gagtctgagc cgtacgacgc ggtcatgcgt
     1201 acggtgggag agccgctgat cgtacacgca gccgggaacg acgcgacctc cggcaatccg
     1261 aacctcacgc tgccgtgggc cccccacaag catattgaag atcaggatcc gaaaacgggc
     1321 gcctacccgc acacatactg ctattcgcag aatggaacgg gtacagactg cacgccggcc
     1381 ttgttcggct gctcgacagg aaccatctat tgcgagacga aaaagcatcc gacacacggc
     1441 gccgacacga ccgtcggttt gatggccagt acgaaaaact cggtgactgt aggcgcgctc
     1501 aatccggatg gcgtctcgat cgcctcgttc agttctcgcg gcccgacgac ggacggacgc
     1561 gtgaagccgg agttggtcgc caagggtgtg tcacagtttt cgacggttcc cgcaaacgga
     1621 tacgacaccc tggatggcac gtcgatggcc acgccggtcg tcgctggcat gtccgctctg
     1681 ctgacgcagc agtatcgaaa gacgttcggc aagacgccct ctgcttcgat tctcaaaacg
     1741 cttctcattg ccggtgcgga cgacctcggc cctgccggtc ccgactacac ctacggcttc
     1801 ggtctggccg atgcgaaagc ctcggtcgat ctcatcatcg ctgacggcgg cacaggctcc
     1861 cggattcgca ccggtacgat catgaatcgc caggatgtcg acattcctgt gtcgctgccg
     1921 gcgacgtcga agttccgagt cgtgctcgga tggttcgatc ccgaggtgct gctggttcct
     1981 gccagtcctg acgacgaccc gcttgcggat aagactctta tcaacgatct cgacgtccgt
     2041 gtcatcgatc ccaccggcgc aaccgtgttt ccttatgttt tgaacggggc gactccgagt
     2101 gcgcttgcta ccaaagccgc caaccatacc gacactacgg aagaggtcga gatcgcaaat
     2161 gcggcagccg gcacgtacca cgtcctcgtc catggcgcga tcggcgatcc gaccggcgcg
     2221 gcgcaggact acgtggtcgt agtcaacgcg gggaccgcgc tcgcgccctg caccgatgcc
     2281 tacgagccga acgatacgca ggacactgct ttcggcaatc tgatgagcgg acagacgatc
     2341 tcggcgaaga tctgcaaccc gctcgatgtc gattacttca agtttacttc gaactcgctt
     2401 tcgccactct cggtgtcggt aaccgcgacc gatacaccgt tgaaggtgac cttgagcagc
     2461 gccttctcca cgacggtcac cacgacgatc gctgccggcg gctccgtgaa cctcggctcc
     2521 gtcatcagca cgccgatctc gccaacgccg aacgtaccct tcttcgtacg cgtcgagccc
     2581 aatggtgcgg tcggcgcgac cggcgcctac acgctgacgc ccaccttcgg ttacaactcg
     2641 attccgcgaa aacgtggggc gaagcattag gggtggggga gtgggtgtcg ggagttagct
     2701 cccggccccc gcgtgaccga agattcttgt tgagtcgata agggccggct gttcgtctcc
     2761 cacgccccgc acccgcatac aatccaacta tgacttacaa aacgcttctc gtcgaagaag
     2821 cggacggtgt gctgacagtc acgctcaacc gtccggacgt tcacaacgcg ttcaatgacg
     2881 agttgatcgc cgaggcgatc gatctcttca gcggcatcgg cgcgacgggc gcgcgggtcg
     2941 ttgtccttcg cggaaccggt ccgaattttt gcgccggcgc ggatctgaac tggatgtcgc
     3001 gcatggtcag ctacacgcgc gaggagaatg tccgcgattc gtcgaagctc gcgcagatgt
     3061 atgcgctgat gaacgagtgt ccgctgccgg tgatcgggcg gattcaggga gcggcgatcg
     3121 gcggcggagt cggtctcgtc gcagtctgcg atatcgccat tgccgcgcgt gacgcgaagt
     3181 ttgggttgtc cgaggtgaag ttgggcatcc ttccggccgt gatctcgccg tacgtgatcg
     3241 ccaagatcgg cgaaacgcat gcccgggcgc tctttctcac cggcgaacga ttcgacgcgg
     3301 agcgcgccca gcggatcggt ctcgtccatc gcatcgtcga cgacgtcgat gccgcaattg
     3361 ccgagacgat cgcaatgctg aaaacgtccg gtcccgaagc cgttcgcgag tgcaagaagt
     3421 tgatcgcgca cgttgcatcg catcccctga tcgacgcgat tccatacacg atcgatgcaa
     3481 tcgcggcgcg gcgcgtaagc gaagaagggc agggcggcat gaccgcgttc ctgaagaagg
     3541 agaaggcgcc atgggtgcgg tagcacctct ctgtcgcccg atgaagcggg cttccaaaac
     3601 gggggtcgcg ttccgagacc cccggctgaa gccgggggct acggtccttt cggcaatctc
     3661 gaccggcgca agatccgtag cccccggctt cagccggggg tttagcggcg ccatcctcaa
     3721 accgagagcc cgctttagcg ggcgacagag gccgcgcgca tgattcgccg tctcctcgtt
     3781 tccaaccgcg gcgagatcgc tgtccgcatc gcgcgggcgt gccgcgcggg cgctacggtt
     3841 tttgctccaa gcgagattgc cgaaagatcc gtagcccccg gcttcagccg ggggttgaag
     3901 ggcgccatcc ccatatcgag atcccgcttt agcgggcgac agagggtgca ctcatgatcc
     3961 gccgtctcct cgtgcccaac cgcggcgaga ttgctgtgag aatcgcgcgg gcgtgccgcg
     4021 aggcgggcgt cgaatcggtg ctggcctact cggagctcga cgacgttcgc tatgtccgac
     4081 gctacttcga cgatgcgatc tccctcggta acggcgacgc gcgcgagaca tacctgaacg
     4141 tcggacgggt catcgattcc gctcgccgtt gtggagccga tgcccttcat ccaggctacg
     4201 ggtttctcag cgagcgcgcc gaactggcag ctgcctgcga tgaggccggc atcatctttg
     4261 tcggtccgaa ggcgagctcg attgcggcga tgggatcgaa agccgcctca cgccatctca
     4321 tgcagcgcct cggcgttccg gtcgttcccg gttacgacgg cgacgatcag aacgtcagca
     4381 cattgaccac cgaagcgtca cgcatcggat ttccgttgat cgtcaaagcg agcgcgggcg
     4441 gcggcggcaa aggaatgaag atcgtgcgct cggccgcgga actgcagagc gcgatcgagt
     4501 cggcgcagcg cgaggcaaag aaatcgttcg gcgatgaccg ccttctgctc gagcgctaca
     4561 tcgaagagcc gcgtcacatc gagttccaga tcttcggcga cggcgagggc aacgtcgttc
     4621 atctcttcga gcgcgactgt tcgatccagc gacggcatca gaaagtgatc gaggagacgc
     4681 ccgcgccgcg ctacagcgag gagcttcgcg cgcgtatggc cgcggcggcg gtggccgcgg
     4741 cacgcggggt cgattatcga agtgccggca cggtcgagtt catcgtcac
//
LOCUS       DATLVW010000125         3887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_458886, whole genome shotgun sequence.
ACCESSION   DATLVW010000125 DATLVW010000000
VERSION     DATLVW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458886"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VNN25_04090"
     CDS             complement(<1..256)
                     /locus_tag="VNN25_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXH90738.1"
                     /translation="MRKLLVLVVIVAAVWFGARALVHRGEVKATVVFDRAGALRQGDP
                     VVADGKTIGVVRKVTHLDDTDAVSIVIDRDHRRDVVSDSLF"
     gene            374..613
                     /locus_tag="VNN25_04095"
     CDS             374..613
                     /locus_tag="VNN25_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXH90739.1"
                     /translation="MSVEDRVKSIIVEQLGVDADEVNPEASFVEDLGADSLDTVELIM
                     AFEEEFGVEISDDEAEKIKKVKDAVEYIDKRAKAS"
     gene            707..1948
                     /gene="fabF"
                     /locus_tag="VNN25_04100"
     CDS             707..1948
                     /gene="fabF"
                     /locus_tag="VNN25_04100"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXH90740.1"
                     /translation="MSKRRVVVTGIGMISPLGVGNEPTWNGLVEGRSGIGPITKFDAS
                     AFTCQIAGEVRGFDPEQWMEKKEVKKSDTFIHYAMAAAEMAVKDASLDFPNCNGERFG
                     VIVGSGIGGLPLIEEMHSKMLERGPSRISPFFIPGLIVNLAAGQISIRYGCQGPSSAP
                     ATACATGAHAIGDAFKVIQRDDADIMFAGGSEAVICKLAVGGFGAMRALSLRNDEPER
                     ASRPWDLNRDGFVIGEGAGILVLEEIEHAKSRGATIYCEITGYGMSSDAHHITSPAED
                     GGGMIRVMRSALKDAQLNPSDIDYINAHGTSTSVGDLTETVAIKGVFGADAYKVAISS
                     TKSMTGHLLGAAGGLEAAVAAMTIKTNIIPPTINYETPDPACDLDYVPNTAREQNLTH
                     VMSNSFGFGGTNATLIFSRLS"
     gene            2042..3049
                     /locus_tag="VNN25_04105"
     CDS             2042..3049
                     /locus_tag="VNN25_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH90741.1"
                     /translation="MIDHTFTPAWFLRGPHAQTIWGRMTRRRRLVKLRRETLTTPDGD
                     ELILDHLDSTVRNDRFRFVLLHGLEGSANSVYMQGMLSVIARLGFAATAVNFRSCARD
                     PKRFGGTLPNRRPRLYHSGETTDFDFVVRTLRARELQVALLAAGASLGGNVLLKWLGE
                     HPGQTDVIAAATMSVPYDLGAGSRHLEIGFIPRFYVTRFLVTLRKKAQQMAAHFPEIR
                     NRIDLEAVARAKTFREFDDAANGPIHGFTGADDYYNRSSSIHFLSAITTPTLCISAAD
                     DPFLPAEVLERAKAIASETVDFRVTTNGGHTGFVAGAWPWQCEYWAEEVLIQWLAGHA
                     K"
     gene            3046..>3887
                     /locus_tag="VNN25_04110"
     CDS             3046..>3887
                     /locus_tag="VNN25_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90742.1"
                     /translation="MNANTNLRRDLLVRLAAAVWGFAIAISLLPLWLGPPYPGQLPGF
                     AKMIGFDARAPMRFALGLMLIPLLWTLALRPLLALLARDDTRDWVRNAFASASAGILW
                     FVIIDRNLAWVLILPAIVVAVSTALRTTDARFSRHDLILLPVLATVYLALLDLVPRSV
                     EQLFVIALTIVFLVRIAIVPIRRTSGLAPSLCFALAPLGLALQSSFNSYDIRHSPWGP
                     LILAIVTPFLLRTFVNDTTAVRSRFRAALRYVIYPLAAYAYLSATSQIAAEGMPRADM
                     FEDQ"
BASE COUNT          696 a         1290 c         1171 g          730 t
ORIGIN      
        1 cgaagagcga atcgctgacg acgtcacggc gatgatcgcg gtcgattacg atcgatacgg
       61 catccgtgtc gtcgagatgc gtaaccttgc gcacgacgcc gatcgtcttc ccgtccgcga
      121 cgaccggatc gccctggcgc agcgccccgg cgcggtcgaa aacgaccgtg gccttgacct
      181 cgccgcgatg gaccagcgct ctggcgccga accacaccgc agcgacgatg accaccaata
      241 ccagcagttt tcgcatcatc gttgccatag gaactcgcga ttgacgattc gcattctgca
      301 ttcgaagttt tttgtgctaa aaaacgccct gcattccccg actcctgtgc ggggaaccca
      361 ggaggagata ccgatgtcgg tagaggatcg cgtcaaatca atcatcgtcg agcaactcgg
      421 cgtcgatgcc gacgaagtga acccggaagc gtcgtttgtc gaggaccttg gagcggactc
      481 gctcgatacc gtcgagctga tcatggcgtt cgaggaggag ttcggcgtcg agatctccga
      541 cgacgaggcc gagaagatca agaaggtcaa ggacgccgtc gaatacatcg acaagcgcgc
      601 caaagcttcg tagccctatc ccggcacggg cctgcccggc ccgtgccatt ccgggaatga
      661 tccacggatc tcccgtgtcc ctacccttgc agattcgcga atacgcatga gcaaacgccg
      721 cgtcgtcgtc accggaattg gaatgatctc gcccctcggc gtcggcaatg agccgacgtg
      781 gaacgggctc gtggaagggc gctcgggcat cggcccgatc acgaagttcg atgcgagcgc
      841 gttcacctgc cagatcgcgg gtgaggtccg cggcttcgat cccgagcagt ggatggaaaa
      901 gaaggaggtc aagaagtccg acaccttcat ccattacgcg atggcagcgg ccgaaatggc
      961 cgtgaaggac gcctcgctcg acttcccgaa ctgcaatggc gagcgcttcg gcgtgatcgt
     1021 cggctccggc atcggcggcc ttcctctgat cgaggagatg cacagcaaga tgctggagcg
     1081 cggcccgagc cggatctccc cgttcttcat ccccggcctc atcgtcaacc tcgccgcggg
     1141 gcagatctcg atccgctacg gatgccaggg accgagctcg gcgccggcta cggcatgcgc
     1201 gaccggcgcg cacgccatcg gcgatgcctt caaagtcatc cagcgcgatg acgccgacat
     1261 catgtttgcc ggcggcagcg aggcagtgat ctgcaagctc gcggttggcg gcttcggtgc
     1321 gatgcgcgcg ctctcgctgc gcaacgatga accggagcgc gcctcacggc cctgggacct
     1381 gaatcgcgac ggcttcgtca tcggcgaagg cgcgggcatc ctcgtgctcg aagagatcga
     1441 gcacgcgaag agccgcggcg cgacgattta ctgcgagatc accggctacg gcatgtcgag
     1501 cgacgcgcat cacatcacgt cgcccgccga ggatggcgga ggaatgattc gggtgatgag
     1561 gagcgcgctg aaggatgcgc agttgaatcc gtccgacatc gactacatca acgcccacgg
     1621 cacctcgacg tcggtcggcg atttgacgga gacggttgcc atcaagggcg tgttcggtgc
     1681 cgatgcatac aaagtcgcga tcagctcgac gaagtcgatg accggccatc tcctcggcgc
     1741 cgctggcggg ctggaagctg ccgtcgccgc gatgacgatc aagaccaata tcatcccgcc
     1801 gacgatcaac tacgaaacgc ccgatcccgc ctgcgatctc gactacgtcc ccaacacggc
     1861 gcgcgagcag aacttgacgc atgtgatgtc gaactccttc ggcttcggcg gcacgaacgc
     1921 gacgctgatc ttcagtcgtt tatcctgagt tggtcgttca aaacggctca ccacagagac
     1981 tcagaggaca ctgagaaacc tctgtgatct cagtgcctct gtggttcatt gataaactca
     2041 tttgatcgac cacaccttca cacccgcctg gttccttcgc ggaccgcacg cgcagacgat
     2101 ctggggccgc atgacccgca ggcgccggct cgtgaagctc cggcgcgaga cgctcacgac
     2161 gcccgacggc gatgagctca tcctcgatca cctcgactcg acggttcgca atgaccgctt
     2221 ccgattcgtc ctgctgcacg gactggaagg aagcgctaac tcggtctaca tgcagggaat
     2281 gttgtcggtc atcgcccgcc tcggcttcgc tgcaaccgcc gtgaatttcc gctcctgcgc
     2341 gcgcgatccg aaacgcttcg gcggcaccct ccccaaccgg cgcccgcgcc tctatcactc
     2401 cggcgagacg accgacttcg acttcgtcgt tcgcacactg cgcgcgcgcg agctgcaggt
     2461 tgctctcctc gccgccggcg cctccctcgg cggcaacgtt ctgctgaaat ggctcggcga
     2521 acatcccgga cagaccgatg tcatcgcggc agccacgatg tccgttccgt acgacctcgg
     2581 cgccggctct cgccatctcg aaatcggatt catccctcgc ttctacgtca cgcggtttct
     2641 cgtgacactc cggaagaagg cgcagcagat ggcggcgcac ttccccgaga tccgcaatcg
     2701 catcgatctt gaggccgtcg cgcgcgcgaa gacgttccgc gagttcgacg acgccgccaa
     2761 cggcccgatt cacggattca ccggcgcgga cgattactac aaccgttcca gctcgatcca
     2821 ctttctcagc gccatcacga cgccgacgct ctgtatcagc gctgccgatg atccgtttct
     2881 gccagccgag gtgctggagc gggcgaaggc gatcgcgtcg gagacggtgg attttcgtgt
     2941 cacgaccaac ggcgggcaca caggtttcgt cgccggggcg tggccgtggc aatgtgaata
     3001 ctgggcggaa gaggtactca tccagtggct ggctgggcac gccaaatgaa cgcgaacaca
     3061 aacctgcgac gcgatctcct cgtccgcctt gccgcggcgg tctggggctt cgcaatcgcc
     3121 atctctctgc tgccgctctg gctcgggccg ccgtatcccg ggcagttgcc gggcttcgcg
     3181 aagatgatcg gcttcgacgc ccgggcgccg atgcgcttcg cgctcggcct gatgctcatt
     3241 ccgctgctct ggacgctcgc gctgcgcccg cttctcgcgc tgctcgcgcg cgacgacacg
     3301 cgcgactggg tgcgcaacgc cttcgcgtcg gcaagcgcag gcatcctctg gttcgtcatc
     3361 atcgatcgca atctggcctg ggtgctgatc ctgcccgcga tcgtcgtcgc ggtatcgacg
     3421 gcgctgcgaa cgaccgacgc acgtttttcg cgtcacgatc tgatcctgct gccggttctg
     3481 gccacggtct atctggcgct cctcgatctc gttccgcggt cggtggagca gcttttcgtc
     3541 atcgcgctga cgatcgtctt cctcgtacgc atcgccatcg tcccgatccg gcgcacgtcc
     3601 ggccttgcgc catcgttgtg tttcgcgctc gcgccgctcg ggctcgcgtt gcagtcgagc
     3661 ttcaattcat acgatattcg gcattcgccg tggggcccgc tgatcctggc gatcgtcacg
     3721 ccatttctcc tgcgcacatt cgtgaatgac acgacggcgg tacggtcgcg gtttcgcgcc
     3781 gcgcttcgct acgtcatcta tccgctggct gcgtacgcgt acctgagcgc aaccagccag
     3841 atcgctgccg aagggatgcc gcgggcggat atgttcgagg atcagca
//
LOCUS       DATLVW010000126         5492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_4503, whole genome shotgun sequence.
ACCESSION   DATLVW010000126 DATLVW010000000
VERSION     DATLVW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4503"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1266
                     /gene="hybB"
                     /locus_tag="VNN25_04115"
     CDS             61..1266
                     /gene="hybB"
                     /locus_tag="VNN25_04115"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HXH90743.1"
                     /translation="MHTQAQPVGGPLFTRSYLLLLGLAGIGIVLILWRFAVGLGAATA
                     LSDGYPLGLWIALDVVTGTALACGGYAVALVVYIFNKGHYHPLVRPAVLTSALGYTIA
                     GASVAVDLGRPWNMWKIPTYFWNWNLNSSLLEVALCIMAYVFVLWIELSPAFLEKFAA
                     DGPARLQRLSKKILPFLNKSLIWIIALGMLLPTMHQSSLGSLMMLAGPKLHPLWLTGW
                     LPLLFLLSCLAMGYAIVVFETVFSSTTFKRNPDMPMLVNLQKIAAWTGVVFVAVRLAD
                     LAVSGNLRYAGQLDFYGSMFWLENVLWILPMIMMLTTSKPTLGSLFRGAMVAALAGAF
                     YRFDTYLVAFNPGPGWHYFPSVSEMFISFGLIAIELAAYIAIVKFFPILSGASLAGNA
                     PKPGSALAS"
     gene            1279..2976
                     /locus_tag="VNN25_04120"
     CDS             1279..2976
                     /locus_tag="VNN25_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXH90744.1"
                     /translation="MTRVTIDPITRIEGHLRIDIEVDQGKVNKAWASSTMFRGIERIL
                     LGRDPREAWLFTQRFCGVCTTVHALASVRAVEDALDLPIPINAQYIRNLTLIAHALHD
                     HIVHFYHLSALDWVDVTQVLKADPAKASSIAESLSDWPNNSTKEMAAVKTKVKALVDS
                     GQLGPFAHGYWGHPAMKLAPEVNLIAVAHYLQALEYQRYANQIVGMLGGKTPHIQNVA
                     VGGVQNAINLDSGAALNLDRLYQMKGLLDKVVSFVQNVYVPDVCAVAGFYPEWFGYGK
                     GVTNYLSVPDLPLDEKSTKFDLPGGFIPNGDLSKFHAITGQKDEYWRKAVTEDSEHAW
                     YKSGGALHPWKGETDPQLTDWKEDAKYSWVKAPRFNGQPAQVGPLANVLAGYAAGNPL
                     MVKWTDAALGKISSIAKVQATPAMLHSTLGRHGARAVRTAVLSDLAVKHWQLLTDNIV
                     KGDTAIYNPPTFPKGEISGVGFHEAPRGTLSHWVVIDDGKIKNYQAVVPTTWNASPRD
                     TSGAVGPYEASLIGNPIADAQHPLEVLRTVHSFDPCMACAVHMFDPAGNNVAKVKVL"
     gene            2973..3425
                     /locus_tag="VNN25_04125"
     CDS             2973..3425
                     /locus_tag="VNN25_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXH90745.1"
                     /translation="MKTAIYGIGNILLGDDGIGPAVVNYLFANKTLGDDVTLEDLGTP
                     SLDLPNYLTGFDRVVFIDAVAVDAPPGTIRLFSRDEIIAAAPGIRISPHEPSINDALI
                     VLDFAGGAPREVVLIGIVPHTLENGMTLSPAVGKAIPRAAMLARALAA"
     gene            3504..4559
                     /locus_tag="VNN25_04130"
     CDS             3504..4559
                     /locus_tag="VNN25_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH90746.1"
                     /translation="MDSAIDLLETRLGANWSAIRAARATTAEVIARLNASLAEFHDAN
                     FSIVVTGSLGRGEATEGSDADWLLLVDGPSDPEHAILARSIGTRIGGIVTKEVGPTGT
                     FGDIVASHELVHYIAGTRDSNENLTRRILLLSESRALTNPLVRERVIRNVLARYVLYD
                     RSVQSGSGKRQTVPHFLLNDVVRYWRTMASDYASKMWERQREGWGIRNVKLRFSRKVL
                     FIGGLLASLAGELFAPPELHEVENDDTYYLLVADLIRTQTDVPPLELLARVVLESDDK
                     SVADSIFSSYDQFLSVLADPEARAKLEAVRFEDAVHDPTYAGLRGASQRFREGVTRLF
                     FDVHPKLPKLIRDFGVF"
     gene            4559..5269
                     /locus_tag="VNN25_04135"
     CDS             4559..5269
                     /locus_tag="VNN25_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90747.1"
                     /translation="MRPIGFSTGALAKGDFRSGLEAQRELDGVDAVELSALRDHELAT
                     LVEATESLDLHGFTYVSFHAPSKLQTLNEKTVFELLLSLPDAWPLVVHPELLQTPSLW
                     RQLGGRLCIENMDNRKATGRTVLELRELFDTYPSASFCLDLGHARQIDPTMASAILML
                     RQFGGRLRQLHVSDVGPFGQHLPLGATARRSFARIAGHIPPDCPIIIESIVATDAIER
                     ELDAVTTVFESVLAQATA"
BASE COUNT         1046 a         1709 c         1620 g         1117 t
ORIGIN      
        1 cgccctgctg ggcgcggtga tgttccgtaa tcgcggcaag tccgacgaga aggagagctg
       61 atgcatacgc aggcacaacc ggtcggtggt ccgcttttca ctcgcagcta tctgttgcta
      121 ctgggcttgg cggggattgg aattgtcctg atcttatggc gcttcgcggt cggactcggt
      181 gccgccactg cgctgtctga cggatatccg ctcggcctct ggatcgcgct cgatgtcgtt
      241 acaggcaccg cgctcgcgtg cggtggttac gccgtcgcgc tcgtcgtcta catcttcaac
      301 aaagggcatt atcacccgct cgtccgcccc gccgttctca ccagcgctct cggctacaca
      361 atcgccggcg catcagtagc agtcgatctc gggcggccgt ggaacatgtg gaagattcca
      421 acctacttct ggaactggaa tctcaattcg tcattgctcg aagtggccct ttgcatcatg
      481 gcctacgtct tcgtgctgtg gatcgagttg tcgccggcgt tcctggagaa atttgctgcc
      541 gacggtccgg cacgtctgca gagactgtcg aagaagattc tcccgttcct caataaatcc
      601 ctcatctgga tcatcgccct cggcatgttg cttccaacga tgcaccagtc gtcgctcggc
      661 tcgctcatga tgctggccgg accgaaactg catccgctct ggctcaccgg ctggctgcct
      721 ctgttgttcc tcctgagctg cctggcgatg ggctacgcga tcgtcgtctt cgagaccgtc
      781 ttctcgtcca ccaccttcaa gcgcaatccc gacatgccga tgctggtcaa cctgcagaag
      841 atcgcggcat ggaccggcgt cgttttcgtc gccgtccggc tggcagacct cgcggtcagc
      901 ggaaatcttc gttacgccgg acagctcgat ttctacggat cgatgttctg gctcgagaac
      961 gtgctgtgga ttctgccgat gatcatgatg ctgacgacgt ccaagccgac gctcggtagt
     1021 ctgttccgag gggcgatggt ggcggcattg gcgggggcgt tctaccggtt cgacacctac
     1081 ctcgtcgcgt tcaatcccgg tccgggctgg cattacttcc cgtccgtttc cgagatgttc
     1141 atcagtttcg gcctgatcgc gatcgagctg gccgcgtaca tcgcaatcgt caagttcttc
     1201 ccgattcttt ccggagcttc gctggctggt aacgccccga aacccggtag cgcgctcgct
     1261 tcataggaga gtacgacaat gaccagagtc acgatcgacc cgatcacacg catcgaagga
     1321 caccttcgca tcgacatcga agtcgatcag gggaaagtca acaaggcgtg ggcctcctcg
     1381 acgatgttcc gcggcatcga gcgcattctc ctcgggcgtg atccgcgcga agcctggctg
     1441 ttcacgcagc gcttctgcgg cgtctgcacg accgtacacg cgctggcctc ggtacgcgcg
     1501 gtcgaagacg cgctcgatct gcccattccg atcaatgcgc agtacatccg caacctcacg
     1561 ctgatcgcgc atgcgctgca cgatcacatt gtccacttct accacctctc tgcgctcgac
     1621 tgggtcgacg tcacgcaggt gctgaaggcg gatccggcga aggcgtcatc gatcgccgag
     1681 agcctctccg actggccgaa caactcgacg aaagagatgg ccgccgtgaa gacgaaagtg
     1741 aaagcgctcg tcgacagcgg tcagctcggt ccgtttgcac acggttattg gggacatccg
     1801 gccatgaagc tggcgcccga ggtgaacctc atcgccgtcg cgcactacct gcaggcgctc
     1861 gagtaccagc gttacgcgaa ccagatcgtc ggcatgctcg gcggcaagac gccgcacatc
     1921 cagaacgtcg cagtcggcgg cgtgcagaac gccatcaatc tcgacagcgg cgcggcgctc
     1981 aatctcgacc ggctctatca gatgaaaggg ctgctcgaca aagtcgtctc cttcgtccag
     2041 aacgtctacg tgcctgacgt ctgcgccgtg gccggctttt atcccgaatg gttcggctac
     2101 ggaaaaggcg tcacgaacta cctctccgtt cccgatctgc cgctcgacga gaagagcacg
     2161 aagttcgatc tgccgggcgg cttcattccg aacggcgatc tctcgaagtt ccacgccatc
     2221 accggccaga aagatgagta ctggcgcaaa gcggtgaccg aggatagcga gcacgcctgg
     2281 tacaagagcg gaggggcgct gcatccgtgg aaaggggaga ccgatccgca gctcaccgat
     2341 tggaaggaag acgccaagta ctcctgggtc aaggcgccgc gcttcaatgg acagcccgcg
     2401 caggttggtc cgctggcgaa cgtgctggcc ggatacgcag ccggcaatcc gctgatggtg
     2461 aagtggacgg acgcagcgct cggcaagatc tcatcgatcg cgaaggttca agccacgccg
     2521 gcgatgctgc actccacgct cggccgtcac ggcgcgcgcg cggtccgcac tgcggtgctc
     2581 tccgatctcg ccgtcaagca ttggcagctt ctgaccgaca acatcgtcaa aggcgacacg
     2641 gcgatctaca acccgcccac gttcccgaaa ggcgagatct ccggcgtcgg tttccacgaa
     2701 gcgccgcgcg gcacgctgtc gcactgggtc gtcattgacg acggcaagat caagaactac
     2761 caggccgtcg ttccgacgac gtggaacgcc agcccgcgcg acacgagcgg cgcggtcggc
     2821 ccctacgaag cgtcattgat cggcaatccg atcgccgacg cgcagcatcc gctcgaggtt
     2881 ctgcgcaccg tgcactcgtt cgatccctgc atggcctgcg cggttcatat gttcgatcct
     2941 gcggggaaca atgttgcaaa agtgaaagtg ctgtgaaaac cgcgatctac ggcatcggca
     3001 acattcttct cggcgacgac ggtatcgggc cggcggtcgt gaactatctc tttgccaaca
     3061 aaacgcttgg cgacgacgtc acactcgaag acctcggcac gccctcactc gacctgccga
     3121 actatctcac cggattcgat cgcgtcgtct tcatcgatgc cgtcgctgtc gatgcgccgc
     3181 cgggaacgat ccgccttttc tcgcgcgacg agatcatcgc cgccgcgccc ggaatccgca
     3241 tcagcccgca cgagccgtcg atcaatgacg cgttgatcgt gctcgacttc gccggcggcg
     3301 cgccgcgtga agtcgtcctg atcggtatcg tgccgcacac gctggagaac gggatgacgt
     3361 tgtcgccggc agtcggcaag gcgattccgc gcgcagcgat gctggctcgg gcgctggccg
     3421 cctaactgaa tgaactgaac ggaacgaaac tacgcactgt agagaattgg ctaacgagag
     3481 ccagggtctg acaaactggc ctcgtggact ccgcaatcga cctgctcgaa acgcgcctcg
     3541 gtgcaaactg gagcgcaatt cgggctgcgc gggcaacgac tgccgaggtg atcgccaggc
     3601 tcaacgcatc actcgcagag ttccatgatg cgaatttctc gatcgttgtg acgggctccc
     3661 tcgggcgagg ggaggcgacc gaggggagtg acgctgactg gttactcctc gtagacggcc
     3721 cttccgatcc agagcatgcg attctcgctc gtagtatcgg gactcgaatc ggaggcatcg
     3781 tcacgaaaga agtcgggcca accggcacct ttggcgacat cgtggcgagc cacgagctcg
     3841 tacactacat cgccggaaca cgcgactcga acgagaacct cacgcgacgc atcctcctgc
     3901 tttcggagtc aagagcgttg acgaatcctc tagttcgcga aagggtcatt cgcaatgtac
     3961 tcgcacgcta tgttctctac gaccgctccg tccagagcgg aagtggaaag cggcagactg
     4021 ttccccattt cctgctgaac gacgtcgtgc ggtattggag aacaatggcc tcggactacg
     4081 catccaagat gtgggaaagg cagcgagaag gttggggcat ccgcaacgtg aagctccggt
     4141 tctcgcggaa ggtgctattc atcggaggcc tcctggcttc gctggcggga gaacttttcg
     4201 cgccgcccga gctccacgag gtggagaacg atgacaccta ttatctgctt gtcgcggatc
     4261 tgatccggac gcagactgac gttccaccac tggaattgct tgcgcgggtg gttcttgaat
     4321 cagacgacaa gagtgttgct gactcgatct tctcttcata cgatcagttt ctgagcgtcc
     4381 tcgccgatcc cgaggccaga gcgaagctcg aggctgttcg gttcgaagat gcggttcatg
     4441 atccgactta cgccggttta cgaggtgcca gccagagatt ccgtgagggc gtcacgcgcc
     4501 ttttcttcga cgttcatcct aaactaccga agttgatccg tgattttgga gtgttttgat
     4561 gagaccaatc ggcttttcaa caggggcgtt ggcgaaaggc gatttcagga gtggcctcga
     4621 agcccagcgc gaactggatg gcgtcgatgc cgttgagctc tcggcgctac gtgatcatga
     4681 gctagcgacg ctggtggaag cgaccgaatc gctcgacctg cacggattca cttatgtctc
     4741 gttccacgcg ccgagcaagt tgcagacact taacgagaag acggtgttcg agttgctgct
     4801 cagcctcccg gatgcatggc cgctggtcgt ccaccctgag ctcttgcaga cgccctcgct
     4861 ttggcgtcaa cttggtgggc gtctttgtat cgagaatatg gacaatcgca aggcgaccgg
     4921 gcggacggtc ctcgagttgc gcgaactgtt tgatacttac ccctcggcaa gcttctgcct
     4981 cgacctcggc cacgcccggc aaatcgatcc gacgatggcc tcggccattc tgatgctccg
     5041 acaatttggt ggccgcctcc gccagttgca cgtgagcgat gtcgggccgt tcggccagca
     5101 tctacccctt ggcgcaaccg ctcggcgctc gttcgcccgg atagccggtc acataccgcc
     5161 ggattgtcca atcatcatcg aatcgatcgt tgcgaccgat gcgatcgagc gtgaattgga
     5221 tgcggtgact accgtttttg aatcggtcct ggcgcaggca acggcgtagc gtccacgctg
     5281 aacactgcgg tcctcctccc cccccccacg tgacatccgt caccacctcc tgtgtcctca
     5341 aacatcgctc cgcgttcgcg ctgcgcctac cttgagcgca cgggaatcgg gacatgacaa
     5401 caggtggact tcatatcgag atccgtggga cggtacaggg agttggttac agaccgtggg
     5461 tgtatcaact ggcgcagcgc gccggggtcc gc
//
LOCUS       DATLVW010000127         5089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_211690, whole genome shotgun sequence.
ACCESSION   DATLVW010000127 DATLVW010000000
VERSION     DATLVW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211690"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..478
                     /gene="rpsK"
                     /locus_tag="VNN25_04140"
     CDS             65..478
                     /gene="rpsK"
                     /locus_tag="VNN25_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXH90748.1"
                     /translation="MATARAPKAGARRAGTKKKERKSIPHGVAAINASFNNTIVAISD
                     PMGNTLSWSSAGRIGFKGSRKGTPFAAQVAAQNAAQLAQEHGVRSVDVRVKGPGAGRE
                     SAIRALAASGLDIKSIKDVTPIPHNGCRPPKRRRV"
     gene            680..1306
                     /gene="rpsD"
                     /locus_tag="VNN25_04145"
     CDS             680..1306
                     /gene="rpsD"
                     /locus_tag="VNN25_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXH90749.1"
                     /translation="MARYSDPVCRLCRREGMKLFLKGDRCFKDKCSIERRNYAPGQHG
                     RRRSKVLGYGIQLREKQKVKRIYGLLEKQFRLYFKRAAGKTGITGDNLLRQLELRLDN
                     VVYSLGFAASRAQARQLVRHGHIEVNGKKINIPSYQGRKGDVIQVREKSRKNDQIRQA
                     VETASGRGIPSWLELNPDQFLGRVNDLPKREDIRLPIQEQLIVELYSK"
     gene            1404..2387
                     /locus_tag="VNN25_04150"
     CDS             1404..2387
                     /locus_tag="VNN25_04150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXH90750.1"
                     /translation="MLWSDFQKPKKLDYDAETLTPNYGRFTAQPFERGFGTTIGNALR
                     RVLLSSIEGAAITAVRVEGVLHEFSSLTGVVEDMTDVVLNLKQVPFRMHGEGPKTLYL
                     EKKGPGVVTAADFEEDSDIEILDPTAHIATLSKEGSLKLEARLKRGRGYQVADRNADG
                     DLPLGYIPIDSIHSPVRRVNYHVEGARVGQMTDYDKLVIEVWTNGAVSPQEAVGLAAD
                     LLGDHLRIFSQFEARGEEAEDVAAPEVDPRMAEMLTKPIEELDLSVRSANCLKNANIR
                     TLGDLVQRTEREMLSTKNFGRKSLDEIKDVLASLGLSFGMTRAPRGAEMRD"
     gene            2422..3012
                     /gene="rplQ"
                     /locus_tag="VNN25_04155"
     CDS             2422..3012
                     /gene="rplQ"
                     /locus_tag="VNN25_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXH90751.1"
                     /translation="MRHGMVNRKLGRTSAHRNALFRNQLASLIDRERIITTLPKAKEL
                     KPQIERLVTLARTDSVHNRRQAARVIADDTKVQKLFSALAQRFADRPGGYTRIIKLGP
                     RRGDAAEMCILEFVDFKLETEAPADETPAKGAKKSAPKKETKAKAEPEEEEDEPKPKA
                     KPKKPAKAAAKPKAEKPKAKEKPPAKKTAAKKKSGK"
     gene            3098..3733
                     /locus_tag="VNN25_04160"
     CDS             3098..3733
                     /locus_tag="VNN25_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HXH90752.1"
                     /translation="MANINLRVVDLYHLDNVTSFQQAADSGLWGVIHKATTGATGQDD
                     AYAKRRQAVLDAGLLWGAYHWGTNADVGQQVDNFLAVAQPDDATLVALDFEETAGNQM
                     TLDQARQFLTLVAGRLGRKPVLYCGGLFKTDMGNTKDAFFGSHRLWLAQYGPTPQPQA
                     SWDSVWLWQYTDSKTGPQPNQVPGIPGDTSGNLDCNSYSFTKEQLAGEWAS"
     gene            complement(3832..4956)
                     /locus_tag="VNN25_04165"
     CDS             complement(3832..4956)
                     /locus_tag="VNN25_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXH90753.1"
                     /translation="MPRVTDDARPDPDELLRLTKTDDKGRGRLKVYLGMAAGVGKTMR
                     MLDDAHRMRRAGVDIAIGLVETHGREETATRIADLEVIPRQKVSYRDVLIEEMDLRAV
                     IARHPDVAVVDELPHTNAPGSKNEKRWQDVEDLLASGISVMTALNVQHLEGVQDVVHS
                     ATGIEIRERVPDRIVREADSVILVDLPVAELQERLRQGKIYPPEQATRALDNFFRTEL
                     LVRLRELALLQTATFSGAPVVVQGAAEVRLAVALPFEPLIAKSLVLRASRLAGRMNTR
                     WYAVYVRQRRAHPENISAEQHRKLSDNVQLAISLGATVVTRESEDVAGALLDFAKSEG
                     ITMLVVGRPTRSGLVSRVVPSTVHRLLERGRGFDIVIVDV"
BASE COUNT         1049 a         1595 c         1558 g          887 t
ORIGIN      
        1 gtgggagatt gaacatcgtt ctccgacacc ccacacccta ctccccactc cccgaggaaa
       61 aacaatggca actgcacgag caccgaaagc tggagcccgc cgcgcgggaa cgaagaagaa
      121 agagcggaag agcattccgc atggcgtggc ggcaatcaac gcctcgttta acaacacgat
      181 cgtggcgatc tccgatccga tggggaacac cctatcctgg tcgagtgccg gccgcatcgg
      241 cttcaaaggc tcgcgcaaag ggacgccgtt cgcggcccaa gtcgcagctc agaatgccgc
      301 gcagcttgca caggagcacg gcgttcgctc ggtcgatgtg agggtcaagg gaccgggagc
      361 cggacgtgag tccgccattc gcgccctggc cgcgtccgga ctcgacatca agtcgatcaa
      421 ggacgtcacg ccgattccgc acaacggttg ccgtccgccg aagcgtcgtc gggtgtagta
      481 aagcagtagt ggatcagtga tcagtcgatt agtggatcag gacaacgatt cccgagtcgc
      541 ctaatctacg aatccactga tcaactgatc tactttttcc ggtacgcgta gttccggtcc
      601 gcagttccgt acggcgtgca gcagccgccg tacacgcgac gttccatggt gggacgggcg
      661 cgaaacttca ggaggtctag tggcacgtta ctcggatccc gtttgccggc tctgccggcg
      721 agagggtatg aagctgtttc tcaagggcga ccgttgtttc aaagataagt gctcgatcga
      781 gcgccgcaat tacgcgcctg gtcagcatgg ccgccgccgt tccaaggtgc tcggctacgg
      841 aatccagctt cgcgaaaaac agaaggtcaa gcgcatttac ggtctgttgg aaaagcagtt
      901 ccgcctttac ttcaaacgcg ccgccggcaa aaccggcatc accggcgata atcttcttcg
      961 ccagctcgag ctccggctcg acaacgtcgt ctactccctc ggttttgccg cttcccgcgc
     1021 tcaggcccgc cagctcgtgc gccacggcca tatcgaagtg aacggcaaga aaatcaacat
     1081 cccttcctac cagggtcgca aaggggatgt catccaggtt cgcgaaaaga gccgcaagaa
     1141 tgaccagatt cgccaggccg tcgagacggc gagcggacgc ggcattccgt cgtggctcga
     1201 gctcaatccc gatcagttcc tcggccgtgt gaacgatctt ccgaaacggg aagacatccg
     1261 cctgccgatc caggaacagc tcatcgtcga actctactcg aagtaaccaa gtctggttgc
     1321 cggctctcag ccgttgactc gcgggatgtt ccgggagtca accgccgagg gccggcagcc
     1381 gatacagcga ggaggccgct cacatgttgt ggtcagattt ccagaaaccc aaaaagctcg
     1441 attacgatgc cgaaactctc acgccgaact acggccgttt taccgctcaa ccgttcgagc
     1501 gcggcttcgg tacgacgatt ggtaatgcgc tccgccgcgt gcttctttcc tcgattgaag
     1561 gcgccgcgat cacggccgtc cgcgtcgaag gcgtgctcca cgagttctca tcgctcaccg
     1621 gcgtggtcga agacatgacc gacgtcgttc tcaatctcaa gcaggtgcca ttccgtatgc
     1681 acggcgaagg tccgaagacc ctctatctcg agaagaaggg accgggcgtc gtcacggccg
     1741 ccgacttcga agaggactcc gacatcgaga tcctcgatcc gacggcacac atcgccacgt
     1801 tgtcgaagga aggctcgctc aagctcgaag cgcgtctcaa gcgcggccgc ggctaccagg
     1861 tcgccgaccg caatgccgat ggcgatctgc cgcttggcta catccccatc gactcgatcc
     1921 attcgcccgt tcgccgcgtg aactatcacg tcgaaggcgc gcgcgtcggt cagatgaccg
     1981 actacgacaa gctcgtcatc gaagtgtgga cgaacggcgc ggtctcgcct caggaagccg
     2041 tcggccttgc cgccgacctc ctcggcgatc accttcgcat cttcagccag ttcgaagcgc
     2101 gcggtgaaga agccgaggat gtggcagcac cggaagtcga tccgcgcatg gcggaaatgc
     2161 tcaccaagcc gatcgaagag ctcgacctct ccgttcgctc cgccaactgc ctcaagaacg
     2221 ccaacatccg cacacttggc gatctcgtgc agcgcaccga gcgggagatg ttgtcgacca
     2281 agaacttcgg ccgcaagtcg ctcgacgaaa tcaaagacgt cctcgcatcg ctcggtctgt
     2341 cgttcggcat gacccgcgcg ccgcgcggcg ccgagatgcg cgactagttt ttccggcgtt
     2401 gaggaaagtg aggggaacgc aatgagacac ggaatggtca atcgcaagct gggacgcacg
     2461 agcgcgcatc gcaacgcgct cttccgcaac cagctcgcct cgctcatcga ccgggagcgc
     2521 atcatcacca cgctgccgaa ggcgaaagag ctgaaaccgc agatcgagcg gctcgtgaca
     2581 ctggcgcgca ccgacagcgt tcacaaccgc cgtcaggccg ctcgcgtcat cgccgacgac
     2641 accaaggtgc agaagttgtt cagcgcgctg gcgcagcgtt tcgctgatcg tccgggcggc
     2701 tacacccgca tcatcaagct cggacctcgc cgcggcgacg ccgccgagat gtgcatcctc
     2761 gagttcgtcg acttcaagct ggagaccgag gcaccggccg atgagactcc tgccaagggc
     2821 gctaagaaat ccgctccgaa gaaggagacc aaggcgaagg cggagcctga ggaagaagaa
     2881 gacgagccca agccaaaggc gaagccgaag aagcccgcga aagcggcggc caagcccaag
     2941 gcagaaaagc cgaaggccaa agaaaagccc ccagccaaga aaaccgccgc caagaagaaa
     3001 agcggcaagt agtcagaagg cccgcctcgt gcgggccttc gcattttccg ggatttgatg
     3061 ggaacatgtt ggtccatttg aatggaggac cagcacgatg gcgaatatca atctccgagt
     3121 cgtcgatctc taccatctcg acaacgtcac cagctttcag caggccgccg attcgggtct
     3181 ctggggcgtc atccacaagg ccacgaccgg cgcaacgggc caggatgatg cgtacgcgaa
     3241 gcggcgccag gcagtcctcg atgccggtct tctctggggc gcctatcact ggggcacgaa
     3301 cgcggacgtc gggcagcagg tggacaactt cctcgcggtc gcgcagccgg atgacgcaac
     3361 gctcgtcgct ctcgacttcg aggaaacggc cgggaaccag atgacgctcg atcaggcccg
     3421 ccagttcctc acgctggtcg cgggacggct tggccgcaag ccggtccttt actgcggcgg
     3481 cctgttcaag acggacatgg gcaataccaa ggacgccttc ttcggctcgc atcgcctctg
     3541 gctggcgcag tacggcccga cgccccagcc tcaggctagc tgggattccg tgtggctttg
     3601 gcagtacacc gattcgaaga ccggcccgca gcccaaccag gttccaggga tcccgggcga
     3661 tacgtcaggc aacctcgatt gcaattcgta cagcttcacg aaggagcaat tggccggcga
     3721 gtgggcgtcg tagctcattg gtcggacaaa aatgcaccac agagacactg aggacacaga
     3781 gttttaacgg ctgctctgtg acctctgtgt ctctgtggtg caaagggctc gttacacatc
     3841 gacaatcacg atgtcgaatc cccgccctct ttccagtagg cggtggacgg tgctcggtac
     3901 gacgcggctg acgagccctg atcgcgtggg gcgtccgact acgagcatcg tgatcccctc
     3961 ggatttcgcg aagtccagaa gcgcaccggc gacatcctcg gattcgcggg tcacgaccgt
     4021 ggcgccgagg gagatggcga gttggacgtt gtcgctgagc ttgcggtgct gctctgcgct
     4081 gatgttctcc ggatgggcgc ggcgctgccg gacgtagacg gcgtaccagc gcgtgttcat
     4141 gcggcctgcc agacgcgatg cgcgtagcac cagcgacttt gcgatcagtg gctcgaacgg
     4201 aagcgcgacc gccagacgca cctcggcggc tccctgcacc acgaccggcg caccggaaaa
     4261 cgtcgccgtt tgcagcagtg ccagctcgcg caaccgcacg agtaactctg tgcgaaagaa
     4321 gttgtcgagc gcgcgggtag cctgctccgg cggatagatc ttcccctgac gcaggcgctc
     4381 ctgaagttcg gcgacgggaa ggtcgacgag gatcaccgag tcggcctcgc gaacgatgcg
     4441 gtccggtacg cgctcgcgaa tctcgattcc ggttgcggag tggacgacgt cctgcactcc
     4501 ttcgagatgc tgcacgttga gcgcggtcat caccgagatc ccgctggcga gaaggtcttc
     4561 cacatcctgc cagcgctttt cgtttttcga gcccggcgca ttcgtgtgcg gcagctcatc
     4621 gacgacagcg acatccggat gccgcgcgat gacggcgcga agatccatct cttcgatgag
     4681 gacatcgcga taggagacct tctgccgcgg aatcacttcc agatcggcaa tgcgcgtcgc
     4741 cgtttcctcg cggccatgcg tttcgacgag accgatggcg atgtcgacgc cggcgcggcg
     4801 catgcggtgg gcgtcgtcga gcatgcgcat cgtcttgccg acgccggcag ccatgccgag
     4861 gtagaccttc agcctgccac gtcctttgtc gtcggttttc gtgaggcgca gcagctcgtc
     4921 gggatcggga cgggcatcgt cggtaacccg gggcatgcgg tcagaatacc tgacaaggtt
     4981 tctggctgcg cggagggtgc gagtgtgaga gcatgcgagt gatggagaca gggtcaatac
     5041 gatgcggccg cgggaaggag catggggcga agccaccgcg ttcttcgag
//
LOCUS       DATLVW010000128         4333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_294286, whole genome shotgun sequence.
ACCESSION   DATLVW010000128 DATLVW010000000
VERSION     DATLVW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294286"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..364
                     /gene="rpsS"
                     /locus_tag="VNN25_04170"
     CDS             71..364
                     /gene="rpsS"
                     /locus_tag="VNN25_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXH90754.1"
                     /translation="MARSLKKGPFIDGHLQKKVDTMNRERDKKVIKTWSRRSTVIPDM
                     VGHTIAVHNGRKFVPVYISENMVGHKLGEFAQTRTFKGHSGKKVEKTAKPAAK"
     gene            379..765
                     /gene="rplV"
                     /locus_tag="VNN25_04175"
     CDS             379..765
                     /gene="rplV"
                     /locus_tag="VNN25_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXH90755.1"
                     /translation="MEATATLKYLKMSPQKVRLVVDLVRGKKVEEALQILRFTKKTCA
                     KDLEKLVRSAVANAEGKEENLDTDELVVSKIYVNEGPREKRIQPAPMGRAYRIQKRQA
                     HVTVHVSDEVKAVNERSGGKGRARAK"
     gene            777..1427
                     /gene="rpsC"
                     /locus_tag="VNN25_04180"
     CDS             777..1427
                     /gene="rpsC"
                     /locus_tag="VNN25_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXH90756.1"
                     /translation="MGQKVNPYGFRLGFNKTWHSRWFAGKAYAETLHRDLKLRDDLKK
                     RLSHAGVSEIDIERTANKMRINIYTSRPGIIIGRKGAEVDKLRDELQKMVNGEVYINI
                     QEIQRPELDAQLVAENIATQLERRIAFRRAMKKAMESAFRFGAKGIKVRVGGRLNGAE
                     IARTEWYQDGRLPLHTLKADIDYGLALARTTYGIIGVKVWIFKGEMAKAKSRKRPQ"
     gene            1525..1950
                     /gene="rplP"
                     /locus_tag="VNN25_04185"
     CDS             1525..1950
                     /gene="rplP"
                     /locus_tag="VNN25_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXH90757.1"
                     /translation="MMMPKKVKFRKQQRGRRRGVATRGAKVSFGDFGLQAMEAGWVTA
                     RQIEAARIAMTRHVKRGGKIWIRIFPDKPITKKPLETRMGKGKGAPEEWVCVIKPARI
                     LYEMEGVTREVAEEALRLAAHKLPIKTRFVTRLQTEVGE"
     gene            1947..2138
                     /gene="rpmC"
                     /locus_tag="VNN25_04190"
     CDS             1947..2138
                     /gene="rpmC"
                     /locus_tag="VNN25_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXH90758.1"
                     /translation="MKTKELRDKSHDELQTRERDLSEQLYKLRFQRATGRMEAPSKVK
                     QVRREIARIKTLQGEKARG"
     gene            2143..2415
                     /gene="rpsQ"
                     /locus_tag="VNN25_04195"
     CDS             2143..2415
                     /gene="rpsQ"
                     /locus_tag="VNN25_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXH90759.1"
                     /translation="MSATEETKPSKNERTKTGVVVSNKMQKTVVVAVENLVKHGMYQK
                     YVKQTSKFLAHAENNDINVGDRVVIEESRPLSKRKRWAVREVIEKA"
     gene            2519..2890
                     /gene="rplN"
                     /locus_tag="VNN25_04200"
     CDS             2519..2890
                     /gene="rplN"
                     /locus_tag="VNN25_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXH90760.1"
                     /translation="MIQMGTVLQVADNTGAKKIACIKMLGGSTAGQYARLGDVITASV
                     KEASPDGTVKKGAVVKAVVVRTVKESRRRDGSYIKFDDNAAVLINDAREPVGTRVFGP
                     VARELRERKFMKIISLAPEVI"
     gene            2955..3308
                     /gene="rplX"
                     /locus_tag="VNN25_04205"
     CDS             2955..3308
                     /gene="rplX"
                     /locus_tag="VNN25_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXH90761.1"
                     /translation="MYGNPTKQREIIRVKPHVKKNDLVLVLSGKDRGKRGKVLRVNAT
                     KGTAVVERVNMMKKHTKANPQKNVSGGILEREAPIKLSNLQVVCPSCGKPARTGTHRT
                     AEGAARYCRKCNTEM"
     gene            3433..3975
                     /gene="rplE"
                     /locus_tag="VNN25_04210"
     CDS             3433..3975
                     /gene="rplE"
                     /locus_tag="VNN25_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXH90762.1"
                     /translation="MAARLKEKYQTEVRKKIQDQFGIKNPMAVPKIEKVVLNMGMGEA
                     ISNAKILDSAVDELGQITGQKPIITKAKKSIASFKLREGQAIGAMVTLRGEKMYEFLD
                     RLISTALPRVRDFRGVPSKSFDGRGNYTLGIRDHLIFPEIDITKVDKSKGMNITIVTN
                     AKDDDQARFLLRELGLPFTK"
     gene            3985..4170
                     /locus_tag="VNN25_04215"
     CDS             3985..4170
                     /locus_tag="VNN25_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXH90763.1"
                     /translation="MAKISMIVKAERKPKFKSRKVNRCKQCGRPRGFLRKFQLCRICF
                     RLLSLGGYIPGITKASW"
     gene            4213..>4333
                     /gene="rpsH"
                     /locus_tag="VNN25_04220"
     CDS             4213..>4333
                     /gene="rpsH"
                     /locus_tag="VNN25_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXH90764.1"
                     /translation="MSMTDPIADMLTRIRNGIQSHHERVELPASKLKVEIARIL"
BASE COUNT         1071 a         1244 c         1350 g          668 t
ORIGIN      
        1 cgaccaaggg tcacaagaca cgcaagaaca agcggacgaa caagttcatc gtcaagcgga
       61 gaaccaagta atggcacgtt cactcaagaa gggcccgttc atcgacggcc acctgcagaa
      121 gaaggtcgac acgatgaacc gcgagcgcga caagaaagtc atcaagacgt ggtcgcgccg
      181 ctccaccgtc atcccggaca tggtcggcca cacgatcgca gtccacaatg ggcgcaagtt
      241 cgtgccggtg tacatctccg agaacatggt cggccacaag ctcggcgagt tcgcacagac
      301 gcgcaccttc aagggccaca gcggcaagaa agtcgaaaag actgcgaagc ctgcggccaa
      361 gtgacacgag gaccaatcat ggaagcaaca gcaactctca agtatctgaa aatgtcgccg
      421 caaaaggtcc ggctcgtagt cgacctcgtg cgcgggaaga aggtcgagga agctctgcag
      481 atcctgcgct tcacgaagaa aacgtgcgcg aaggacctgg agaagctggt gcgctcggcg
      541 gttgcgaacg ccgagggcaa ggaagagaac ctcgacaccg acgaactggt ggtctcgaag
      601 atttacgtca atgaggggcc gcgcgagaag cgtatccagc cggcgccgat gggacgtgcc
      661 taccggattc agaagcggca ggcgcacgtg acggtgcatg tgtcggacga agtcaaagcg
      721 gtcaacgagc ggagcggcgg caaaggccgc gcccgcgcaa agtaacggag gctatcttgg
      781 gtcagaaggt caacccgtac ggatttcggc ttggtttcaa caagacgtgg cactcccgct
      841 ggtttgcggg gaaggcttac gccgagacgc ttcatcgtga tctgaagctt cgcgacgatc
      901 tgaagaagcg gctgtcccat gccggagtgt cggagatcga catcgagcgc actgcgaaca
      961 agatgcgcat caacatctac acctcgcgcc cgggcatcat catcggacgt aagggcgccg
     1021 aggtcgacaa actgcgcgat gagctgcaga agatggtcaa cggcgaggtg tacatcaaca
     1081 tccaggagat ccagcgtccg gagctcgacg cccagctcgt ggccgagaac atcgccaccc
     1141 agctcgagcg ccgcatcgcc ttccggcgcg ccatgaagaa ggccatggaa tcggctttcc
     1201 gcttcggcgc gaagggaatc aaggtccgcg tgggcgggcg cctgaacggc gccgagatcg
     1261 cccgcacgga gtggtaccag gatggccggc ttccgctgca cacgctgaag gcggacatcg
     1321 attacggcct ggcgctggcc agaacgacgt acggcatcat cggcgtcaag gtatggattt
     1381 tcaaaggcga aatggcgaaa gccaagtcgc ggaagcgccc gcagtagtga gatcgggcgt
     1441 cgggcgtcgg gtgccggtag tcggagctga gttctccgac ccccgacccc cgacctccga
     1501 ctcccgcaca ggagaaaccg catcatgatg atgccgaaga aggtcaagtt cagaaaacag
     1561 cagcgcggtc gccgtcgtgg tgtggcgact cgtggtgcga aggtgtcgtt cggcgacttc
     1621 ggtctgcagg cgatggaagc gggctgggtt acggcgcgcc agatcgaggc agctcgtatc
     1681 gcgatgaccc gtcacgtcaa gcgcggcggc aagatctgga tccgcatttt cccggacaag
     1741 ccgatcacga agaaaccgct cgagactcgt atgggtaagg gaaaaggtgc accggaagaa
     1801 tgggtgtgcg tcatcaagcc ggcccggatc ctctacgaga tggaaggcgt gacgcgcgag
     1861 gtcgctgaag aagcgcttcg cctggccgca cacaaacttc cgatcaagac acgcttcgtc
     1921 acgcgtctgc agacggaggt gggtgaatga aaacgaaaga gcttcgcgat aagagtcacg
     1981 acgagcttca gacccgcgag cgcgatctga gcgagcagct ttacaagctt cgctttcagc
     2041 gtgccacggg ccggatggaa gcgccgtcga aagtgaaaca ggtgcgccgc gagatcgccc
     2101 gcatcaaaac cctccagggt gaaaaggcga ggggctaaga ccatgtcagc aactgaagaa
     2161 accaaaccca gcaagaacga gcgcaccaag accggcgtcg tcgtatcgaa caagatgcag
     2221 aagacggtcg tcgtcgccgt cgagaacctc gtcaagcacg ggatgtatca gaagtatgtg
     2281 aagcaaacga gcaagttcct cgcgcacgcc gagaacaacg acatcaacgt cggcgaccgc
     2341 gtggtcatcg aggaatcgcg tccgctgtcg aagcggaagc ggtgggccgt gcgcgaagtg
     2401 atcgagaagg cgtagtaacc gacaaggatg agggatgaag gacgaaggat gaagactgga
     2461 tgattgctcg agcattcatc cttcatccct ccgccttcat cctttgggag aaacggcaat
     2521 gattcaaatg ggaactgtcc tacaggtcgc ggacaacacc ggagcgaaga agatcgcctg
     2581 catcaagatg ctgggcggat cgactgccgg ccagtacgcg cgcctcggcg acgtgattac
     2641 ggcgtcggtc aaggaagcct cgcccgatgg caccgtcaag aagggcgctg tcgtgaaggc
     2701 ggtcgtcgtc aggacggtca aagagagccg ccgccgcgat ggctcgtaca tcaagttcga
     2761 cgacaacgcg gccgttctca tcaacgacgc gcgcgagccg gtcggcacac gcgtctttgg
     2821 cccggttgcc cgcgagctgc gcgagcggaa gttcatgaaa atcatttcac tggcaccaga
     2881 ggtcatctag aaaacaggtc ccggcggtca ggttcggttc tccgaccccg acagccggca
     2941 gccggagcaa aacaatgtac ggaaatccga cgaagcaaag agaaatcatt cgcgtcaaac
     3001 cgcatgtgaa gaagaacgat ctcgtcctcg tcctttcggg caaggaccgc ggcaagcggg
     3061 gcaaggtgct gcgtgtgaac gcgacgaagg gaacagccgt cgtcgagcgc gtcaacatga
     3121 tgaagaagca caccaaggcc aatccgcaga agaacgtttc gggcggcatt ctcgagcgcg
     3181 aagcaccgat caagctttcg aacctgcagg tggtctgccc cagttgtggc aagccagccc
     3241 gcaccggcac ccatcgcacc gctgaaggcg cagcgcggta ctgccggaag tgcaatacag
     3301 agatgtagta acgggcgtcg gggatcgggt gtcgggagtc ggagccagaa catctccgac
     3361 tcccgacccc cgaaacgcga ctcccgcaaa cgtggccgta caacgccggc ccggaaggaa
     3421 ccaggaaaga ccatggcagc gcgactgaaa gagaaatatc agaccgaagt gagaaagaag
     3481 attcaggacc agttcgggat caagaacccg atggcggttc cgaagatcga gaaggtcgtc
     3541 ctcaacatgg ggatgggcga agcgatctcg aacgccaaga tcctggacag tgctgtcgac
     3601 gagctgggcc agatcacggg ccagaagccg atcatcacca aggccaagaa gtcgatcgcc
     3661 agcttcaagc tgcgcgaggg acaggccatc ggcgcgatgg tgacgctgcg cggggagaag
     3721 atgtacgagt tcctcgaccg tctgatctcc acggcgctgc cccgggttcg tgacttccgc
     3781 ggcgttcctt cgaagtcgtt cgacggacgc ggcaactaca cgctcggcat ccgcgaccat
     3841 ctgatcttcc ccgagatcga catcaccaag gtcgacaagt ccaaggggat gaacatcacc
     3901 atcgtcacga acgcgaagga cgacgatcag gctcgtttcc tcctccgcga gctcggtttg
     3961 cccttcacca aataagagga acacatggca aaaatctcga tgatcgtcaa agcggaacgg
     4021 aagccgaagt tcaagtcccg caaagtcaat cgctgcaaac aatgcggacg tccgcgcggt
     4081 tttctccgga agtttcagct ctgccgcatc tgcttccggc tgctttcgct cggcggctac
     4141 atcccgggca tcacgaaggc gagctggtag tcccggctct caaagactga ataggaatca
     4201 caggagtcac gaatgtcgat gacagaccca atcgcggaca tgctcacgcg gattcgcaac
     4261 ggaatccagt cgcatcacga acgcgtcgag cttcccgcgt cgaagctgaa agtggagatc
     4321 gcccgcatcc tca
//
LOCUS       DATLVW010000129         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_172828, whole genome shotgun sequence.
ACCESSION   DATLVW010000129 DATLVW010000000
VERSION     DATLVW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172828"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1448
                     /locus_tag="VNN25_04225"
     CDS             261..1448
                     /locus_tag="VNN25_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90765.1"
                     /translation="MKRLILSLAMIAAVGCASPQQQKTPPAAQPPSFQPSYGNDMGTS
                     PVGVIPSAMLHDGLRNKDVEVSIEYPTRGAGPFPVIIFSPGYGGSSHGYEGLISYWTS
                     YGYVCIRPMHADAGALGDTMRDLLAMRRDDRPTRNQQRRDRARVDAEAKAKPPANLAE
                     MIWDKEREPQWRDRVRDVTVILDALNDLEKNYPELAGKMDHARIGVGGHSYGAFTAML
                     IGGTKTFGNPPLALGDPRVKAIVAMSPQGIAANRGLTADSWKDLRIPAMFMTGTRDYG
                     ADQTEGPDWHRLGFDHSPAGDKYFVLIRGANHMTFTGVASGLGEQFEPNPPMIDPRTG
                     QQMYPTQQPSNRGSVITDRGTFASIRSISLMFWDSYLKNKKEAKELLDPSKYAGGVEV
                     TRK"
     gene            complement(1463..2131)
                     /locus_tag="VNN25_04230"
     CDS             complement(1463..2131)
                     /locus_tag="VNN25_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HXH90766.1"
                     /translation="MNIALFDIDGTLTESNAIDSLCFADAFRDLFGVQIDTDWDSYQH
                     TTDRGIVVEALRRARGREADAHEIALHKARFVQLLAERMMELNEIPGAAAFLAQILAR
                     NWRVVLCTGAWGESARLKLERAEFPTELPLASCDDDVAREGILRRGIALAGGGVGDVV
                     VSFGDAPWDVRAAASLRLPFIGIAAGVRAESLRRSGAAEVFADFEDAAAVFAGIDRCV
                     RCSA"
     gene            2313..2564
                     /locus_tag="VNN25_04235"
     CDS             2313..2564
                     /locus_tag="VNN25_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXH90767.1"
                     /translation="MAFVITDNCIKDALCVDACPVDCIHPRMDEASFEGVTQMYVDPA
                     ECIDCGACIPACTSDALFALDDVPADKQQFIAANAAYFA"
     gene            2649..>4064
                     /locus_tag="VNN25_04240"
     CDS             2649..>4064
                     /locus_tag="VNN25_04240"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXH90768.1"
                     /translation="MTDKKIIWTNVDEAPALASYSLLPIVRAFTKGTGVAVETGDISL
                     AGRILASFPERLTEAQRVPDDLARLGALTLSPEANIIKLPNISASIPQLKEAIRELQE
                     QGYDVPDYPEVAASDAEKAIQARYAKVLGSAVNPVLREGNSDRRAPLAVKNFARKNPH
                     KLSGWNAGSKAEVAYMSEGDFYGNEQSIVMDTGGAFRIELAGNDGAVTILKDGLTATP
                     GEILDGTFMSVKALRAFYGAQIEDARSNGQLLSVHLKATMMKVSDPILFGHAVRVFFK
                     AVFEKHAASLRDAGVNPNLGLGDLYRKIQSLQEAKRAEIEADIQATYAKRPALAMVDS
                     DNGITNLHAPNDIIVDASMPVVIRDAGKMWNADGKLQDTKALIPDRCYATMYEEIIEN
                     CKKFGALDPATMGSVPNVGLMAQKAEEYGSHPTTFEIPFEGVVRVVDANGNVLMRHKV
                     EAGDVWRMSRAKDVPIQDWVKL"
BASE COUNT          818 a         1298 c         1260 g          688 t
ORIGIN      
        1 agcagggtgg gggagtgggg agtggggagt ggggtgtggg gggggtcgga gcggcagaga
       61 tcagagataa tcagagatca gaggtcgaag atcggagcgt ctagaccaat ctccgactcc
      121 cgacccccga cgtcctccga cccccgactc ccactctcca ccccccactc cccaccccct
      181 gtcacgctcc tgtgagaatg ccgcccattg attttgcatt catcaaggtt gatgtacgtt
      241 catttgcact cggaggctcg atgaagcgac tcattctgtc cctcgccatg attgccgcgg
      301 tgggatgcgc atctccccag cagcagaaaa cgccgcccgc ggcgcaaccg ccgtcgttcc
      361 agccgtcgta cggcaacgac atgggcacct cgccggtcgg cgtcattccc tccgcgatgc
      421 tgcacgacgg cctgcgcaac aaggatgtcg aggtatcgat cgagtatccg acgcggggcg
      481 cgggaccgtt tccggtcatc atcttctcgc ccggatatgg cggatcgagc cacggctatg
      541 aagggctgat ctcgtactgg acgagttacg gctacgtctg catccggccg atgcacgccg
      601 acgccggcgc gctgggcgac acgatgcgcg accttctggc gatgcggcgt gatgaccggc
      661 cgacgcggaa tcagcagcgg cgcgaccggg cgcgcgtcga tgccgaggcc aaggcgaaac
      721 cgccggcgaa tctggcggaa atgatctggg acaaagagcg cgagccgcag tggcgcgacc
      781 gcgtgcgcga cgtcaccgtc atcctcgatg cgctcaacga tctggaaaag aactacccgg
      841 aactggccgg caaaatggat cacgcccgca tcggcgtcgg cggccattcc tatggcgcgt
      901 tcacagcgat gctgatcggt ggcacgaaga ccttcggcaa tcctccgttg gccctcggtg
      961 atccgcgtgt gaaggcgatc gtggccatgt caccgcaagg cattgccgca aaccgcggac
     1021 tgaccgccga ttcctggaag gacctccgca tccccgcgat gttcatgacc ggcacgcgcg
     1081 attacggcgc cgaccagacg gaaggccctg actggcaccg cctcggcttc gaccactcgc
     1141 ccgccggcga caagtacttc gtcctcattc gtggcgcgaa ccacatgacc ttcaccggcg
     1201 tagcgtcagg cctcggcgaa cagttcgagc cgaacccgcc gatgatcgat ccgcgcacgg
     1261 gccagcagat gtacccgacg cagcagccat caaaccgcgg ctccgtcatc accgaccgcg
     1321 gcactttcgc ctcgatccgc tcgatctcgt tgatgttctg ggactcgtat ctcaagaaca
     1381 agaaagaggc gaaggaactg ctcgatccat ccaagtatgc ggggggcgta gaggtgacga
     1441 ggaagtaggg ggcggtcgct gctcaggcgc tacaacggac gcaccgatcg ataccagcaa
     1501 agaccgccgc tgcatcctca aagtcagcga acacctcggc ggctccgctc cgccgaagac
     1561 tctctgcgcg cacgcccgca gcgatgccga tgaagggaag tcggagactc gccgctgcgc
     1621 gcacgtccca tggtgcgtcg ccgaaggaga cgacgacatc gccgacgccg cctcccgcca
     1681 gtgcgatgcc gcgccgcagg atgccttcgc gcgcgacgtc gtcatcacac gacgccagcg
     1741 gcagttcagt ggggaattcc gcgcgctcca gcttgagccg cgccgattca ccccacgcgc
     1801 cggtgcaaag cacgacacgc caattccgtg caaggatttg agcaaggaaa gcggcggccc
     1861 cgggaatctc gttcagctcc atcatccgct ccgcgagcag ttgtacgaaa cgcgctttgt
     1921 gcagcgcgat ctcatgagcg tccgcctcgc gtccgcgcgc ccgccgtagc gcctcgacca
     1981 cgatgccgcg atcggtcgtg tgctgatagg aatcccaatc cgtgtcgatc tggacaccga
     2041 agaggtcgcg gaacgcatcc gcaaagcaga gcgagtcgat cgcgttgctc tccgtcagcg
     2101 tgccgtcgat atcgaaaagg gcgatgttca ttgtttatcc gcagatgacg cagagaggcg
     2161 cagatcgaga ggatctcatc tgcgttcatc tgcgtcatct gcggaccggc aactgtgatc
     2221 tctgtcatac caaacagtga cccgacaaac acatccacgt ggcatcgccc gctaccatcg
     2281 ctctcgaaat ctagctttca ggagacgatc aaatggcctt cgtgatcact gacaactgca
     2341 tcaaagacgc gctgtgcgtg gacgcctgtc cggtcgactg cattcatccg cggatggatg
     2401 aggcttcgtt cgagggagtc acgcagatgt acgtcgaccc tgcggagtgc atcgactgtg
     2461 gcgcctgcat tccggcctgc acctcggacg cactatttgc gctcgacgac gtgcccgcgg
     2521 acaagcagca gttcatcgct gcgaatgcgg cgtacttcgc atagcgttac gcccctcgtg
     2581 tagtacgacg gaacgccgcc gtcccggcgg ctggcccggc ggcgtcctcg ccgccgcaag
     2641 gaacgattat gaccgacaag aagatcatct ggacgaacgt cgacgaagcg ccggcgctgg
     2701 catcgtattc actgcttccg atcgtgcggg cgtttacgaa agggacgggc gtcgccgtcg
     2761 aaacgggcga catctccctc gcggggcgga tcctggccag cttcccggag aggctgacgg
     2821 aagcgcagag ggttccggat gatctggcgc ggctcggcgc gctcacgctg tcgcccgagg
     2881 ccaacatcat caagctgccg aacatcagcg cttcgattcc gcaattgaaa gaagcgatcc
     2941 gcgagctgca ggaacaaggg tacgacgtcc ctgactatcc cgaggtggct gcgagcgacg
     3001 ccgaaaaggc gatccaggcg cgctatgcga aggttctcgg cagcgcggtg aatcccgtcc
     3061 tccgcgaagg gaactctgat cgcagagctc cgctggccgt gaagaacttc gcccgcaaga
     3121 atcctcacaa gctgagtggg tggaatgccg gctcgaaggc cgaagtcgcg tacatgagcg
     3181 agggcgactt ctacggcaac gagcagtcga tcgtcatgga caccggcggc gcgttccgga
     3241 tcgagctcgc cggcaacgat ggcgcggtca cgatcctgaa ggacggtctg accgcgacgc
     3301 cgggcgagat tctcgacggc accttcatga gcgtgaaggc attgcgcgcg ttttacggcg
     3361 cgcagatcga agacgcccgc agcaatgggc agctgctctc cgttcatctc aaggcaacga
     3421 tgatgaaggt gtccgatccg attttgttcg gtcacgccgt ccgcgttttc ttcaaggctg
     3481 ttttcgagaa gcacgctgcc tccctgcgcg atgccggcgt caatcccaac ctcgggctcg
     3541 gcgatctcta ccgcaagatt caatcgctgc aggaagcgaa gcgcgccgag atcgaagccg
     3601 atatccaggc cacgtacgca aagcgtccgg cgctggcgat ggtcgactcc gacaacggca
     3661 tcacgaacct ccatgcgccg aacgacatca tcgtcgacgc ctccatgccg gtcgtcattc
     3721 gtgacgccgg aaagatgtgg aacgctgacg ggaagctgca ggacacgaag gctctcatcc
     3781 ccgaccgctg ctacgccacg atgtacgagg agatcatcga gaactgcaag aagttcggcg
     3841 cgctcgatcc ggcgacgatg ggctccgtgc cgaacgtcgg cctcatggcg cagaaggcgg
     3901 aagagtacgg atcgcatccg acgacgttcg agattccctt cgaaggcgtg gtccgtgtcg
     3961 tcgatgcgaa cggcaacgtg ctgatgcggc acaaggtcga agcgggcgac gtctggagaa
     4021 tgtcgcgagc caaggacgtt ccgatacagg attgggtgaa gctg
//
LOCUS       DATLVW010000130         6456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_339174, whole genome shotgun sequence.
ACCESSION   DATLVW010000130 DATLVW010000000
VERSION     DATLVW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339174"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3421)
                     /locus_tag="VNN25_04245"
     CDS             complement(<1..3421)
                     /locus_tag="VNN25_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH90769.1"
                     /translation="MSTSHAIVFYVNGNRVEIADPDPTTLLIDWLRSDAIGFTGTKLS
                     CGEGGCGACTVMLSRWEPKERRVAETAVNSCLRPLCSLDGAMITTTEGIGSTREKLDP
                     VQYAIAANNGSQCGFCTPGFVMNMFTFLRNNPKPEQQQIEDLFDGHLCRCTGYRPILE
                     GMKSFAVDSRKHSTYSTNHKCFADVPIEQHLPGPRPELEFPPELIEYANAMSSLESAG
                     RGYRWFRPANLAEAQQIKAANGGNSANVKLVVGNTSIGIYKADVADPHVLIDIHAIPS
                     LLGFRDSDKGLDIGAAESLQNVIDELQRVIATKPKEKTRGFAAFRTHLLDVANLQVRN
                     IGSIGGNVMMTRAQAETDSPFPSDVYLVLETLGASITIASRDYDGGIKTFAMAELPAV
                     STLPADAIAQSFHIPYSQAGDEIETFKIAYRDQNAHAIVNAGFVVRLDKKGVVESATI
                     LYGGLAAMPSRMTKTEAAITGAKWTDATLQKALDVLAAEVGSVIRPIPGTNFLPDGYR
                     ESLTETLFYKFFLHVANERFPKEVQPENRSGGEVYVRPLSGGTQTVSVYHQERPVGEP
                     ILKTTAFVQAAGEQKYTQDIPLPPHGYDSAFVISEIASGTFGYKTDATAALKAAQSMY
                     DGVVAYITADDIPNKKNPYLGLGGDDVVFALNQQIICWGQPIGLVVAKDRWTAQRAAY
                     FIQTEMIDFAPTKPVLTIDEAVAAKDFFTDQQYNEHIPYITRKGSDEKWLKNPTQPMK
                     GCNVVEGEMGNEAQEHFYMETQGALALPDAQGAMTIYAGTQQGSSVQSAVAQLLEIEN
                     SAVTVFQRPLGGGFGGKQARPLVINPAPAIAAWILNRPVRLILDRNTDMNVTGKRHPY
                     QGHFHATFTKKGEIKGYKVQLYSNGGSTYDLSFPVMDLSQQHSDGAYFVPTWHSRGDV
                     TRTNNASNTAFRSFGVVQATLICEEAIEKIAHACGMRAEDVRWKNMYQDGSKRSYQLT
                     PYAQKLDQCNIRDLWKRLMKSSDFNKRRDGVDKFNAKNRWRKRGLTMIPIKYGVGYQP
                     RLLDQGVAYVAAYAADGSVMLQHGGAESGQGIDTKMMQIAAETLGIDMSIIRVAQTST
                     DTMPNATPTAASSGSDLFGGAVRNACLELRARLEKYCKENKVKGWKTEWKKL"
     gene            complement(3431..4390)
                     /locus_tag="VNN25_04250"
     CDS             complement(3431..4390)
                     /locus_tag="VNN25_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXH90770.1"
                     /translation="MTRIPVLIDTDVDIDDWMAILYLSMHPNVDLRGITVTGVGACHL
                     TPGTRNALNLLQLTGKPDVPVAKGTSAPLSYSNVFPQSIRGPIDAVYNVPLPANPTAP
                     VTEGAVDFLRATLMASSTPMTILAIGGGTNLGTLLNNYPDVAPKIERIVMMGGAVYVP
                     GNVNVVDPDYTNQVAEWNFFLDPLGGQYMFDSGVPVTLIPLDASDHVPLNLPFYDRIQ
                     QRHGTPQAAFIYQTLTADIPFVQSGTFYFWDPLAAAVLTDPGLASGKTLKLRVVQELD
                     EEADHSGQLVEDPDGASIDVMLDADADAFYDLFLDVINGSING"
     gene            4506..5453
                     /locus_tag="VNN25_04255"
     CDS             4506..5453
                     /locus_tag="VNN25_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXH90771.1"
                     /translation="MDVILVHDAAIDEYMSQVLLTTMPGINLLGAVIVNADCIDSQAM
                     QTAWQIMSYIGRTDIPMGLSSARGWNPFPWSYRSDCTRESEINCLKSFPPNPAWPPFP
                     DGDALLQSLMEKAAGKVTLLVTCPFTPIMNLLQKSPALNAKIEQIIWMGGAIHVGGNL
                     DPATVPTPPWNKCAEWNAFWDAVSVEWVFKNTTCPIIQFPLDMTNRATIATSFMETLA
                     VQAHNGSRYSELAFESYMLVTYETFYDMWDVVTTCWLTSPKFFEPPKTMHLTIDTALN
                     NTQACIKQAPGGREVQVVLNFSKDGQQAFYDYVAKQFNR"
     gene            5505..>6456
                     /locus_tag="VNN25_04260"
     CDS             5505..>6456
                     /locus_tag="VNN25_04260"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH90772.1"
                     /translation="MSSIFRVTRRQFLRDIGVGTGALVLGCSIVPESVFAAERTRTTV
                     APGLAFNAYVAITSLHGDIIITTHRSEMGQGIRSSLAAVLADELEADWSRVTLRQADA
                     DAVSFEIPLFYGTTNASLELASTPALIKGEDAQQTEASQSMALYYMPMRLFGAAIRVV
                     MLRAAARKLQVDPAELEASQQKVWHRPTNRSVEYHRLLLQAGRVPLPTTDEVKAALKP
                     ANQWRFMGRNMPFMDARDMVTGKAVYGADVETGRRGMLTAMIVRCPVANGALKSFDPS
                     AALAVRGVKFVEPVLSPGFLRGMTGGVGANYVPHAGVAVLA"
BASE COUNT         1130 a         1978 c         2067 g         1281 t
ORIGIN      
        1 agagcttctt ccattccgtc ttccagccct tgaccttgtt ctccttgcag tacttctcga
       61 gacgcgcacg cagctcgaga caggcatttc gcaccgcgcc gccgaacagg tccgatcctg
      121 atgacgccgc agtcggcgtt gcattcggca tcgtgtcggt cgaggtctgg gcgacgcgga
      181 tgatgctcat gtcgatgcca agagtttccg cggcgatctg catcatcttg gtgtcgatgc
      241 cctgacccga ctccgcgccg ccgtgctgca gcatcacgct tccgtcggct gcgtacgccg
      301 ccacatacgc gacgccctga tcgagcagcc gcggttgata gccgacgccg tacttgatcg
      361 ggatcatcgt caggccgcgc ttgcgccagc ggttcttcgc gttgaacttg tcgacgccgt
      421 cacggcgctt gttgaagtcg gacgacttca tcagccgctt ccagagatcg cggatgttgc
      481 actgatcgag cttctgcgca tacggcgtaa gctgatacga gcgcttcgat ccatcctggt
      541 acatgttctt ccaccgcaca tcctcggcgc gcataccgca cgcatgcgcg atcttctcga
      601 tggcctcctc gcagatcagc gtggcctgca cgacgccgaa actgcggaac gcggtgttgc
      661 tggcgttgtt cgtccgcgtc acgtccccgc ggctatgcca cgtcggcacg aagtacgcgc
      721 catccgagtg ctgctgcgaa agatccatga ccgggaagct gaggtcatac gtcgatccgc
      781 cgttggagta gagctgcacc ttgtagcctt tgatctcgcc cttcttcgtg aacgtggcgt
      841 ggaagtggcc ctgataggga tggcgcttgc cggtgacgtt catatccgta ttgcggtcga
      901 ggatcagccg cacgggacga ttgagaatcc acgcggcgat cgccggcgct gggttgatta
      961 ccagcgggcg cgcctgcttg ccgccgaatc cgccgccgag cggacgctga aagacggtga
     1021 cggcgctgtt ctcgatctcc aggagctgcg cgacggcgga ctgcaccgac gatccctgct
     1081 gcgtcccagc gtagatcgtc atcgccccct gcgcgtcggg caatgcgagc gcgccctgcg
     1141 tctccatgta gaaatgctcc tgcgcctcgt tgcccatctc cccttcgacg acgttgcatc
     1201 ccttcatcgg ctgcgtcgga ttcttgagcc acttctcgtc gcttcctttg cgggtgatgt
     1261 aggggatgtg ttcgttgtac tgctggtcgg tgaagaagtc cttggcggcg acagcctcat
     1321 cgatcgtcag caccggcttc gttggcgcga agtcgatcat ctccgtctgg atgaagtacg
     1381 cggcgcgttg cgcggtccag cgatccttcg ccacgaccag tccgatcggc tggccccaac
     1441 agatgatctg ttggttgagc gcgaagacga cgtcgtcgcc gccgaggccg aggtatgggt
     1501 tcttcttgtt ggggatgtcg tcggcggtga tgtaggcgac cacgccgtcg tacatggact
     1561 gcgcagcctt caacgcggcc gtcgcatctg ttttgtagcc gaaagtaccg ctggcgatct
     1621 cgctgatgac gaacgcggag tcgtaaccgt gcggcggcag cgggatgtcc tgcgtgtatt
     1681 tctgctcgcc ggcggcctgc acgaacgcgg tggtcttgag gatcggctcg ccgacggggc
     1741 gctcctggtg atagacgctc accgtctgcg tgccaccgga gagcggccgc acgtaaacct
     1801 cgccgccgga gcgattctca ggctgcactt ccttcggaaa gcgttcgttg gcgacgtgga
     1861 gaaagaactt gtagaagagc gtttccgtga gcgactcgcg atagccgtcc ggcaggaagt
     1921 tcgtgcccgg gatcgggcga atgacgcttc cgacttccgc cgcgagcaca tcgagcgcct
     1981 tctgcagcgt cgcgtcggtc cacttcgcgc cggtgatggc cgcctccgtc ttcgtcatcc
     2041 gcgacggcat cgccgcgagt ccgccgtaga gaatcgtggc cgactcgacg acgcctttct
     2101 tgtcgagccg gacgacgaag ccggcattca cgatcgcgtg cgcgttctga tcgcgatagg
     2161 cgatcttgaa cgtctcgatc tcgtcgcctg cctgcgagta cggaatgtgg aatgactggg
     2221 cgatggcgtc ggcgggaagc gtcgacaccg ccggcagttc ggccatcgcg aaggtcttga
     2281 tgccgccgtc gtaatcgcgc gacgcgatgg tgatcgatgc gccgagggtt tcgagcacga
     2341 ggtagacgtc ggaagggaat ggagagtccg tctccgcttg cgcgcgcgtc atcatcacgt
     2401 tgccgccgat cgagccgata ttgcgcacct gcagattggc gacgtcgagg agatgcgtgc
     2461 ggaacgccgc gaagccgcgc gtcttttcct tcggcttcgt cgcgatgaca cgttgcagtt
     2521 catcgatcac gttctgcaat gactccgcgg caccgatatc gaggcccttg tcggagtctc
     2581 ggaatccgag gagagaggga atggcgtgga tgtcgatgag gacgtgcggg tcggcgacgt
     2641 cggccttgta aatgccgatc gaggtgttgc cgaccaccag cttgacgttc gccgagttgc
     2701 cgccgtttgc ggccttgatc tgctgcgcct ccgcgaggtt tgcgggacgg aaccagcgat
     2761 aacctcggcc tgcgctttcc agcgacgaca tcgcgttcgc gtactcgatc agctcgggcg
     2821 ggaactcgag ctcgggacgc ggtcccggca gatgctgttc gatcgggaca tcagcaaaac
     2881 atttgtggtt cgtcgagtac gtgctgtgct tcctgctatc gacggcgaag ctcttcatcc
     2941 cctcgaggat cgggcggtag ccggtgcagc ggcagagatg gccgtcgaag agatcctcga
     3001 tctgctgctg ttcgggcttt ggattgttgc gcaggaacgt gaacatgttc atcacgaacc
     3061 cgggtgtgca gaagccgcac tgcgatccgt tgttcgcggc gatcgcgtac tgcacgggat
     3121 cgagtttctc gcgcgtgctt ccgatcccct cggtggtcgt gatcatggcg ccgtcgagcg
     3181 aacagagcgg acgcagacac gaattgaccg cggtctcggc gacgcgccgt tccttcggct
     3241 cccagcgcga gagcatcacc gtgcaggcgc cgcagccgcc ttcgccgcag gagagcttcg
     3301 tgccggtgaa accgatggca tcgctgcgaa gccaatcgat gagcagcgtg gtcggatcgg
     3361 gatccgcaat ctcgacgcgg tttccgttga cgtagaagac gatcgcgtgg gatgtgctca
     3421 tgcgctctcc ttacccattg attgaaccat tgatcacatc gaggaagagg tcgtagaagg
     3481 catcggcgtc cgcgtcgagc atcacgtcga tcgatgctcc atccggatct tcgacgagct
     3541 ggccggagtg gtccgcttct tcgtcgagct cctggacgac gcgcagcttc agcgtctttc
     3601 cgcttgccag gcctggatcg gtcaggaccg ccgccgcgag cgggtcccag aaatagaacg
     3661 tcccgctctg cacgaagggg atgtccgcgg tgagcgtctg gtagatgaac gccgcctgcg
     3721 gcgtaccgtg gcgctgctgg atgcgatcgt agaacggcag attcagcggc acgtggtcgg
     3781 acgcatcgag cggaatgagc gtgaccggca cgccggagtc gaacatgtac tggccgccca
     3841 gcggatcgag gaagaagttc cactcggcga cctgattcgt gtagtcggga tcgacgacgt
     3901 tgacgtttcc gggaacgtag accgcacctc ccatcatgac gatgcgctcg atcttcggcg
     3961 cgacgtcagg gtagttgttg aggagcgtgc cgaggttcgt gccgccgccg atggcgagga
     4021 tggtcatcgg agtagaagac gccatcagcg tcgcgcgcag aaagtcgaca gcgccctccg
     4081 tcacgggcgc ggtcgggttg gccggaagcg gaacgttgta gacggcgtcg atcgggccgc
     4141 gaatcgattg cgggaagacg ttgctgtagg agagcggcgc gctcgtcccc ttcgcgaccg
     4201 ggacgtccgg cttgccggtg agctgcagaa ggttgagcgc attgcgcgtc cccggcgtga
     4261 gatgacatgc gccgacgccg gtgacggtga tgccgcggag atcgacgttc ggatgcatcg
     4321 agagatagag gatggccatc caatcgtcga tgtcgacgtc ggtgtcgatg agcacaggga
     4381 ttcgcgtcat gcgtccgcct ttcgtgtcgt gttcttcggg gatgccgcca tcctaaagcg
     4441 agctgcctcg gcccgcaaga atgttaggat gcagatccac ccaaaaactt cggaaggagc
     4501 atcccatgga cgtcatcctt gtgcacgacg ctgcgatcga cgagtacatg tcgcaggtcc
     4561 tcctcaccac catgcccggc atcaatctcc tcggcgccgt catcgtcaac gccgactgca
     4621 tcgactccca ggccatgcag accgcctggc agatcatgag ctacatcggc cgcaccgaca
     4681 tccccatggg gttgagcagc gcgcgcggct ggaatccgtt tccgtggtcg taccgcagcg
     4741 actgcacgcg cgagtcggag atcaactgcc tcaagagctt cccgccgaat ccggcatggc
     4801 cgccattccc cgatggcgac gcgctgctgc agtcgctgat ggaaaaggcc gccggcaagg
     4861 tgacgctgct cgtgacctgt ccattcacgc cgatcatgaa tctgctgcag aagtcgccgg
     4921 cgctgaatgc gaagatcgaa cagatcatct ggatgggcgg cgcgatccat gtcggcggca
     4981 atctcgatcc ggccaccgtt ccgacgccgc cctggaacaa atgcgcggag tggaacgcct
     5041 tctgggatgc cgtctcggtc gaatgggtct tcaagaacac gacctgtccc atcatccagt
     5101 tcccgctgga catgaccaat cgagccacaa tcgccacgag cttcatggag acgctggccg
     5161 tacaggccca caacggctca cgctactcgg agctggcgtt cgagagctac atgctggtga
     5221 cctacgagac gttctacgac atgtgggatg tcgtcactac ctgctggctc acgagtccga
     5281 aattcttcga gccaccgaag acgatgcacc tgaccatcga tacggcgctg aacaacacgc
     5341 aggcctgcat caagcaggcg ccgggcggcc gtgaagtgca ggtggtgctg aacttttcga
     5401 aggacggtca gcaggcgttc tacgactacg tggcgaagca gttcaatcgg tgaggttagg
     5461 cgcggcctgg tccacgtccg accgcgccgt gtgtcaactt ggcgatgagt tctattttca
     5521 gagtcacgcg caggcaattc ctccgcgaca tcggcgtggg tacaggagcg ctggtgctcg
     5581 gttgttcgat tgtgcccgaa tcggtttttg ccgctgagcg gacgaggacg actgtggctc
     5641 cgggcctcgc attcaatgcg tacgttgcca tcacatccct gcatggggac atcatcatca
     5701 ccacccaccg ttccgagatg ggacagggca tccgatcgag cctcgcggcg gttcttgccg
     5761 acgagctcga ggcggactgg agccgggtaa cgctgcgaca ggctgacgct gatgccgtga
     5821 gtttcgagat ccctcttttc tatggcacaa ccaacgcttc tctcgagctc gccagcactc
     5881 cggcgcttat caaaggcgag gacgcacagc aaaccgaagc ttcgcaaagc atggccctgt
     5941 actacatgcc catgcgcctc ttcggggcgg ccatccgggt ggtgatgctc cgtgcggccg
     6001 ccagaaagtt gcaagtcgat ccggccgagc tcgaggcctc gcagcagaag gtgtggcaca
     6061 gacccacgaa ccgttccgtc gaataccaca gactgctgtt gcaggccggc agggtgcctt
     6121 tgcccacgac ggacgaggtc aaggccgcgc tcaagccggc caatcagtgg cgcttcatgg
     6181 gcaggaacat gccgttcatg gacgcccggg acatggtcac cgggaaagcg gtctacggcg
     6241 ccgacgtcga aaccggccgg cgcggcatgc tgaccgccat gattgtccgc tgcccggtag
     6301 ctaacggcgc gttgaagtcg ttcgacccgt ccgcggcgct cgcggtgcgc ggcgtgaagt
     6361 tcgtggagcc ggtcctttcc ccggggttcc tccgcggcat gaccggcggg gttggcgcaa
     6421 actacgttcc gcatgccggc gtggcggtat tggcag
//
LOCUS       DATLVW010000131         8830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_252357, whole genome shotgun sequence.
ACCESSION   DATLVW010000131 DATLVW010000000
VERSION     DATLVW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252357"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3728
                     /locus_tag="VNN25_04265"
     CDS             <1..3728
                     /locus_tag="VNN25_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXH90773.1"
                     /translation="HTPPPTPHTPVTIIDEGHRRAIDLALEIPRSNVETVMSNEVWGE
                     YYDRLTQLIGEHKTTLVFVNTRRMAERAARHLSERLGDDAVTAHHGSLSKEKRLDAES
                     RLKNGQLKALVATASLELGIDIGHVDLVCQIASPHRIATLLQRVGRSGHSVSGLPKGR
                     LFPTSLDDLIECAALMREVRRGELDRIVTHDAPLDVLAQQIVAETSCEDYTEDELFAL
                     VRRAWPYRDLARKNFDEVLAMVAEGFATKRGRRGALVHRDEVNHRVRGRRGSRMLAIT
                     SGGAIPEVADYKVVLEPDDTVVGTLNEDFAIESNAGDIFQLGNASWQITQVLSGTVRV
                     SDAKGAPPTIPFWLGEAPARSDELSRAVSEMRADLGMILSGGGEWGVGSGSDRPDDQS
                     NQLSETALPHPTPHSPPPDHRLPAQHWLISQTGMPPEAAREIVAYLDDSRRALGALPT
                     QDTLILERFFDESGGMQLVLHAPFGSRINKAWGLALRKRFCRQFNFELQAAATEDAIL
                     LSLGPQHSFPLADVFRYLHPSTARDVLIQAMLAAPVFPTRWRWNTTISLAIPRNRGGR
                     KTPPPLQRAMADDLMAAVFPDAAACPENLGGDRELPDHPLVNQVVRDCLEEAMDFDGL
                     ARVLTRIHAGEIECIARDTTEPSPLAHEILNAKPYAFLDDAPLEERRTQAVMTRRTTA
                     NDLGALDPSAIARVREEARPDPRDADELHDALLTFGFLLEGEVDAPELFTELAAARRA
                     TVLFGQEHRQECLCHTTPASEDGLCDIMPGRDRLSLRSGSDVAQTLLSVQAPAGIWIA
                     AERIPEILAIHPTITPDIDPPASRKNKEWSREEAVVEIIRGRISLLGPTTATELAASM
                     HIAESDADAALLKLEQEGLVLRGSFTPHPPLPTPQQWCDRRLLARIHRYTLNRLRAEI
                     EPVTATDFTRFLFAWQHVTRSTRLTGIDGLHEVIKQLDGFEIAMNAWERHILPLRIDR
                     YEPSLLDSLCFSGEVGWARLTTGVALAPRSHIPAWLTPESPPPALSENAQRVLEALQT
                     RGASFARDLDADQKTAQETIDELANAGLITSDGFIGRRPERAGRWSLLAASTDREESV
                     ETQARALLRRYGVIFRRLLTREPNAAPWRELARVYRRLEARGEIRGGRFVAGMSGEQF
                     ALPESVERLREIRRSAPDGRLIVISAADPLNLIGILTTGERIRAIPGTRIAYRDGIAL
                     SVMEGDFLRPLAEPDMDVAMALAGRRVPVAAGFVGRA"
     gene            complement(4124..4864)
                     /locus_tag="VNN25_04270"
     CDS             complement(4124..4864)
                     /locus_tag="VNN25_04270"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXH90774.1"
                     /translation="MTASLFELQLHARPSNLGTPRPRTRNHETLRPPFDVHAISVPAR
                     MFTGDFLFTHRAADRLWFALGDVAGKGLPAALVMAMIQEELERRIASCARAGCDPATT
                     MRRLHEFLEPLLPSNRFATAVIGWLRDNGTMTIVNAGHCPPMLVRADGRIEEIDSTGP
                     VVGIMAASGWISATFTLNAGDQLILYSDGVIEAASPDGEEFGVEGIVQALPAFASSRD
                     VASAIHAAVSQHANGARYDDLSLLVIGR"
     gene            complement(4978..5835)
                     /locus_tag="VNN25_04275"
     CDS             complement(4978..5835)
                     /locus_tag="VNN25_04275"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXH90775.1"
                     /translation="MTRIDALRTRELASIEKTAVEQHFKTCRSCNDSQADVVDLADAI
                     KSLAVAPERSCREAVKEQIADSFDRVGDAWVAFSKGRIRMIAKAQSIDDVREAYAKKY
                     GRVLEPGTIPDALRRQVEAAVKGEGVATPKVDLDAANDLEHDVMQTLVRIPRGEVRTY
                     AWVARQAGRPRAVRAVASIIARNAVPYVVPCHRVVPSAGGVGNYIFGSATKRALLERE
                     GVNVDELDELAREHVRYIGSRTTHIFCFPTCRDAKRIQEKNRMPFLDADDAMEHGYRP
                     CRHCQPVAA"
     gene            complement(5843..6475)
                     /locus_tag="VNN25_04280"
     CDS             complement(5843..6475)
                     /locus_tag="VNN25_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH90776.1"
                     /translation="MKNSWTGGELAGEPDRREGGDLAVAVHRCEPGAFERLIDRFERP
                     LFGYAHGILQNAFDAQEVVQDAMMRAHKALTRQYDEARCAALALKPWLFKTVRNLCLN
                     KRRSKRSSLEQPLETFDDGRIGPFVSLEGSDLERKEEIELLRRAMALLPVDARELIVL
                     RFMEEMSYAEIAKTVGQSEAALRGRVFRSLKLLRDALEKPATQKGVAYAM"
     gene            6752..7333
                     /locus_tag="VNN25_04285"
     CDS             6752..7333
                     /locus_tag="VNN25_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXH90777.1"
                     /translation="MRKLKIIEHISLDGVIQHSADDGDFPYSDWTAPYRTPAGREAVL
                     AAQGESFDLLLGRRTYDIWSGFWPKAPRSPMADGINAATKFVATHRPESLEWGPVEGL
                     GPDIVAGIRRIKSQDGPNLILWGSSTLTSTLLEHGLADEVLLIVYPLLLGTGKRFFAE
                     GTPARSFELVSTNAMPSGNILSTYKVAGPLKTG"
     gene            8523..>8830
                     /locus_tag="VNN25_04290"
     CDS             8523..>8830
                     /locus_tag="VNN25_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90778.1"
                     /translation="MNPNQETINPSAAADLQQAAPTLSPEGVVDQLRATRAQIGEMTP
                     LTVAQRKALHGRIRTSNPILQASINVIGAHDIVAKAIGQPADDMRLLCDETNRWTAVE
                     "
BASE COUNT         1558 a         2976 c         2779 g         1517 t
ORIGIN      
        1 cccacacccc accccccaca ccccacaccc cagtaaccat catcgacgaa ggccaccgcc
       61 gcgccatcga tctcgcgctc gagatcccac gttcgaacgt cgagacggtg atgtcgaacg
      121 aggtttgggg tgagtattac gaccggctga cgcagctcat cggcgagcac aagaccacgc
      181 tcgtcttcgt caacacgcgg cggatggccg agcgggcggc gcggcatctc agcgagcggc
      241 ttggcgacga tgcggtgacg gcgcatcacg gcagtctgtc gaaagagaag cggctcgatg
      301 ccgagtcgcg tttgaagaac gggcagctca aagcgcttgt ggcgaccgca tcgctggagt
      361 tgggcatcga catcggtcac gtcgatctcg tctgtcagat cgcgtcgccg catcgcatcg
      421 ccacgctgct gcagcgcgta gggcggtcgg gccactcggt gtccggtttg ccgaagggcc
      481 ggctcttccc cacctcgctc gatgacctca tcgaatgcgc ggcgttgatg cgcgaagtgc
      541 gacgcgggga gctcgatcgc atcgtcacac atgacgcgcc gctcgatgtg ctcgcgcagc
      601 agatcgtggc cgagacgtcg tgcgaggact acaccgagga tgagctgttc gcgctggtgc
      661 gcagggcgtg gccgtatcgc gatctggcgc ggaagaattt cgatgaagtg ctggccatgg
      721 tcgcagaagg cttcgccacg aagcgcgggc ggcgcggcgc gctcgtccat cgcgacgaag
      781 tgaaccatcg cgttcgcgga cgccgcggct cgcgcatgct ggccatcacg tccggcggcg
      841 cgatccccga ggtggcggat tacaaggtgg tgctggagcc ggacgatacg gtcgtcggca
      901 ccctcaacga agacttcgcc atcgagagca acgccggcga catcttccag ctcggcaacg
      961 cctcgtggca gatcacgcag gtcctgagcg gcaccgtgcg cgtgtccgac gccaaaggcg
     1021 cgccgccgac gatccctttc tggctcggcg aagcgcccgc gcgcagcgat gagctgtcgc
     1081 gcgcggtgag cgagatgagg gcggatctgg ggatgatcct ttcggggggt ggggagtggg
     1141 gggtggggag tgggagtgat cgacctgacg atcaatcaaa tcagctatca gaaaccgcgc
     1201 tcccacaccc caccccccac tccccacccc ccgaccaccg actccccgcc caacactggc
     1261 tcatctccca aaccggcatg ccgcccgagg ccgcgcgcga gatcgtcgcc tacctcgacg
     1321 actcacgccg cgcgcttggc gcgctaccga cgcaggacac gctcatcctc gaacgtttct
     1381 tcgacgaatc gggcggcatg cagctcgttt tgcacgcgcc attcggcagc cgcatcaaca
     1441 aagcgtgggg gctggcgctg cgcaaacgct tctgccggca gttcaacttc gagttgcagg
     1501 ccgcggccac cgaggatgcg attctgttgt cgctgggacc gcagcactcg tttccgctcg
     1561 cggatgtttt ccgctatctg cacccatcga cagcgcgtga cgttctgatc caggccatgc
     1621 ttgccgcgcc ggtgttcccg acgcgctggc gctggaacac tacgatctcg ctggctatcc
     1681 cccgcaatcg cggcggccgc aagacgccgc cgccgttaca gcgagcgatg gccgacgacc
     1741 tcatggccgc cgtctttcct gacgccgcgg cctgccccga aaacctcggc ggcgaccgcg
     1801 agctgccgga tcatccgctc gttaatcagg tcgtccgcga ttgcctggag gaggcgatgg
     1861 acttcgacgg cctcgcccgc gtgctgacgc gcatccacgc cggcgagatc gaatgcatcg
     1921 cccgcgacac caccgagccg tcgccgctcg cgcacgagat cctcaacgcc aagccgtacg
     1981 cgttcctcga cgacgcgccg ctcgaggagc gacgcaccca ggccgtcatg acccgccgca
     2041 ccaccgccaa cgaccttggc gccctcgatc cctccgcgat cgcgcgcgtc cgcgaggaag
     2101 cgcgtcccga tccgcgcgat gcggatgaat tgcacgatgc gctgctgaca ttcggattcc
     2161 tgctcgaagg cgaagtcgat gcgccggagt tgttcacgga actggcggcg gcaagaaggg
     2221 cgactgtgtt gtttggtcag gagcacagac aagagtgtct gtgccacacc actcctgcct
     2281 ccgaagacgg tttgtgcgac atcatgcccg gccgcgaccg gctgagctta agatccggaa
     2341 gtgatgtggc acagacactc ttgtctgtgc aggccccggc aggtatctgg attgccgccg
     2401 aacgaatccc cgagatcctg gccatccatc cgacgattac ccccgatatc gacccacccg
     2461 catcgcgcaa aaacaaagaa tggtcccgcg aagaagccgt cgtcgagatc atccgtggcc
     2521 gcatctccct cctcggcccg acaactgcaa ccgagctggc cgcatcgatg cacatcgccg
     2581 aatccgacgc cgacgccgcc cttctcaaac tcgagcagga aggcctcgtc ctccgcggtt
     2641 cttttactcc ccacccccca ctccccaccc cccaacagtg gtgcgaccgc aggctcctag
     2701 cccgcatcca tcgctacacg ctcaaccgct tgcgcgccga gatcgaacca gtcaccgcca
     2761 ccgacttcac gcgcttcctc ttcgcctggc agcacgtcac cagatcgacg cggctgaccg
     2821 gaatcgacgg tcttcacgaa gtcatcaagc aactcgatgg cttcgagatc gccatgaacg
     2881 cgtgggagcg gcacatcctc ccgctgcgca tcgatcgcta cgaaccatcg ctgctcgatt
     2941 cgctctgttt cagcggcgaa gtgggatggg cgcggctgac caccggcgtc gccctcgcac
     3001 cgcggtcgca catccccgcc tggctcacgc ctgagtcccc gccgccagcc ttgagcgaaa
     3061 acgcgcagcg cgttcttgaa gcactccaaa cacgcggcgc atctttcgct cgcgatctcg
     3121 atgccgatca aaaaacggct caggaaacga tcgacgagct ggcgaacgca ggcctcatca
     3181 cctcggacgg cttcatcgga cgccgccccg agcgcgccgg acgctggtcg ttgcttgccg
     3241 cgagcaccga ccgcgaggaa tctgtcgaaa cacaagcccg cgcgttgctg cgaagatacg
     3301 gagtcatctt ccgccgcctg ctgacgcgcg aaccgaacgc tgctccctgg cgcgagcttg
     3361 cccgcgtcta ccgccgcctc gaagcaagag gcgaaatccg tggcggccgc ttcgtcgccg
     3421 gcatgtccgg cgaacagttc gcgctccccg agtcggtcga gcgactgcgc gagatccggc
     3481 gaagcgcgcc cgacggacgc ctcatcgtca tcagcgccgc cgacccactc aacctcatcg
     3541 gcatcctcac caccggagaa cgaatccgcg ccatccccgg cacgcgcatc gcctaccgcg
     3601 acggcatcgc gctatccgtg atggaaggcg acttcctgcg cccgttagcc gagccggaca
     3661 tggacgtagc aatggcgctt gcgggacggc gagtgccggt tgcggcgggg tttgtggggc
     3721 gggcgtaacg cggagggacg aaagagaaag agtggaagcg gttcgataat gagatcggca
     3781 ctcctgccga agcaggattc tagctttcgc taatcgatgc cccaggacgt agatctcgaa
     3841 cccggtattt cgatcattcg taaccgcttc cgcggagaaa tctacgtcgt cggcgccgcc
     3901 gaggcaaccg gtcgattagc gcaacacatc ggcggcgaga cgccgccggg ccagccgccg
     3961 agacggcggc gttccgtccg aaggatcatt tgccacttta cgcgcgtccg gtgagcaagg
     4021 ctcgcccgtc caaaggatcc tttgccactt gcggcggaac gctggcgtcc cgccggctgg
     4081 ctcggcggcg tcctcgccgc cgcggttctc gccgctgcgc cgcctaccgc ccgatcacga
     4141 gcagcgatag atcgtcgtat cgcgcgccgt tggcgtgttg cgacaccgcg gcgtggatcg
     4201 cggacgcgac gtcgcgcgac gacgcaaacg ccgggagagc ctgcacgatc ccttcgacgc
     4261 cgaactcctc gccgtccggc gatgctgctt cgatgacgcc gtcgctgtag agaatcaact
     4321 gatctcctgc gttcagcgtg aacgtcgctg atatccagcc cgaggcggcc atgatgccga
     4381 cgaccgggcc ggtcgaatcg atctcttcga tgcggccatc ggcgcgaacg agcattggtg
     4441 ggcagtggcc ggcgttgacg atcgtcatcg tgccgttgtc gcgaagccag ccgatgacgg
     4501 cggtggcgaa gcggtttgac ggcaggagcg gctcgagaaa ctcgtgcagg cgtcgcatcg
     4561 tcgtcgctgg atcgcagccg gcgcgcgcgc acgaagcgat gcggcgctcc agctcttcct
     4621 ggatcatcgc catcacgagc gcggcaggga gtcccttccc tgccacgtcg ccgagggcga
     4681 accagagacg gtcggcggcg cggtgggtga acaagaagtc gccggtgaac atgcgcgctg
     4741 ggacggagat ggcgtggaca tcgaatggcg gccgcaaggt ctcgtgatta cgcgtgcgtg
     4801 gtctcggcgt cccgaggttg ctcggtctcg cgtgaagttg aagctcgaaa agtgaagcgg
     4861 tcatgatgtt ctccgtttta gaaagcctgc gatcctcgtg cttcggcagg ggattcctcg
     4921 cccaccgccg ccccgcccgc tcggaatgac aggcggaggc ctcgaacccc gcaaccgcta
     4981 cgccgccacc ggttggcagt ggcggcaggg acggtagccg tgctccatcg cgtcgtcggc
     5041 atcgaggaag ggcatccggt tcttctcctg aatccgtttc gcgtcgcggc aggtcgggaa
     5101 gcagaagatg tgcgtcgtgc gggagccgat gtagcgtacg tgctcccgcg ccaactcgtc
     5161 gagctcatcg acattcacgc cctcgcgttc cagcaacgcg cgcttcgtgg cgctgccgaa
     5221 gatgtagttg ccgacgccgc ccgccgaggg cacgacgcga tggcacggca cgacgtatgg
     5281 aacggcgttg cgggcgatga tgctggccac ggcgcggacg gcccgaggac ggccggcctg
     5341 acgggcaacc caggcatagg tgcgcacttc gccgcgcgga atgcggacga gtgtctgcat
     5401 cacgtcgtgc tcgagatcgt tcgcggcatc gagatcgacc ttcggcgttg cgacgccttc
     5461 acccttcacc gcggcttcca cctgccgccg taacgcgtca gggatcgttc caggctcgag
     5521 cacgcgtccg tactttttcg cgtaggcctc gcgaacgtca tcgatcgact gcgctttggc
     5581 aatcatgcgg atgcggccct tggaaaaggc aacccacgca tcgccgacac ggtcgaaaga
     5641 atcagcgatc tgctccttca ccgcctcgcg gcaggagcgc tccggcgcga cggccagcga
     5701 tttgatcgcg tccgcgaggt cgacgacgtc cgcctgcgaa tcattgcaac tgcggcaggt
     5761 cttgaagtgc tgctccaccg ccgtcttctc gatcgaggca agctcgcgtg tgcgcagcgc
     5821 gtcgatcctg gtcaataccg ttctacattg catacgccac ccctttctgg gttgccggtt
     5881 tctccagcgc atcgcgcagc agcttgagcg accggaacac cctgccgcga agcgccgcct
     5941 cggactggcc taccgtcttt gcgatctccg cgtacgacat ctcctccatg aaccgcagca
     6001 cgatcagctc gcgcgcgtcg accggcagca aagccatggc ccgccgcagc agctcgatct
     6061 cttccttccg ctcgagatcc gacccctcga gactgacgaa cggcccgatc cggccatcgt
     6121 cgaaggtctc cagcggttgc tcgagcgagc tgcgtttcga gcgccgcttg ttgagacaaa
     6181 gattgcgaac ggtcttgaaa agccagggct tcaacgccag cgccgcgcag cgggcctcgt
     6241 cgtactgccg cgtgagcgcc ttgtgcgcgc gcatcatcgc gtcctgcacg acttcctgcg
     6301 catcgaacgc gttctgcaga atgccgtggg catagccgaa gagcggtcgt tcgaaccgat
     6361 cgatgagcct ctcgaacgcc cccggctcgc agcgatggac ggccacggcg aggtcgcccc
     6421 cttcacgccg atcaggctcc cccgccaatt cgccgcctgt ccaactgttc ttcatgttgc
     6481 taacaacacg ggtgaaggga gtttcgttcg ggagcattca ttttgtgacg agccagcttt
     6541 ccagcgtagc gccgacggcc tcgtcggctg gtccgccgcc ggcccggcgg caactccttg
     6601 caagaaccgc cagcgagccg ctggcgatac agccgacgag gccgtcggcg ctacgctgcg
     6661 taactaccct gctcgttcac gggattcgtc ggttgcatgc tttctgagaa gacacagtgg
     6721 ttacaattcc atcggcaggt aacctcatcg catgagaaag ctcaaaatca tcgaacacat
     6781 ctcgctcgac ggcgtgatcc agcactccgc cgatgacggc gatttcccct acagcgactg
     6841 gaccgcgccc tatcgaaccc ccgctggccg ggaggcggtg ctcgccgcgc agggcgagag
     6901 cttcgatctg ctgcttggcc gtcgcaccta cgatatctgg tcgggcttct ggccaaaagc
     6961 gccgaggagt ccgatggcgg acggcatcaa tgcggccaca aagtttgtcg ctacccaccg
     7021 tccggagagt cttgaatggg ggccggtcga gggacttgga ccggacatcg tcgcgggcat
     7081 tcgccggatc aagtcgcagg acggcccgaa ccttatcctc tggggtagct ccacgctgac
     7141 atcgacgctg ctcgaacatg ggctggcgga tgaagtcctg ctgatcgtct atccgctcct
     7201 gttgggcacg gggaagcgct tctttgcgga ggggaccccg gcacgctcat tcgagctcgt
     7261 cagcacgaac gcaatgccgt ccggcaacat cctcagtacc tacaaggtcg ccgggccgtt
     7321 gaagaccgga tagttcaacg acgaagaagc cggtggggtc acaccttcgc acttcggtcg
     7381 ttccggatcg tgctcgccta agcctctctc tgagagtgac ttaccactcg gatacaattc
     7441 acggcaccgc aggctgcgag agcccacaaa gcagccaagc gatgcgtttg cagcaactta
     7501 ggcacgaatt cgagcctgcg cgcgtggcga cgccttccgg gtgtggctga cgcaagtcga
     7561 tgaagccgcg cgagttatgc ccacgctcga ggagattagc gaagaccgaa ggtgtgaccc
     7621 caggaacgcc gagctcgcga accgcgcggc gtcaagccgg acgtgtgggg cgtccggtca
     7681 gacgcatgcg gcgtccatcc gcgcgtgtgg aacgtccagt cagacgtgtg cggcgtcgat
     7741 ccgcacgtgt gaggcgtccg gtcagacgcg tgcggcgtcc atccgcgcgt gtggaacgtc
     7801 cagtcagacg tgtgcggcgt ccatccgcgc gtgttgggcg tccggtcaga cgtgtgcggc
     7861 gtccatccgc gcgtgtgggg cgtccagtca cgggtgcggc gtcgatccgc acgtggtggg
     7921 cgtccagtca gacgcgtgcg gcgttgatcc gcacgtgtgc ggcgcccggg cggacctgtg
     7981 gatcgtccag ctcagacggt gagtcgctca gccggcgcgg acggtcatcg ggcgcgagcg
     8041 gtaactcatt acgacgacgt ggccgggcgc ccggtgtacg cgtaaggtcg tcgggtttgc
     8101 gctgacagca attggtgttt gccgatgttg tgtcctcgga atgccagcgg cgactcagta
     8161 acgcagcatg cacggcgacg ccgccaactt gacatcgccc accaggctct gctgcaatac
     8221 gcggcctcgt ggggccggct tgccggcttc ttgagactgg agccggcgct cgtgagagtc
     8281 ccacgctcct ggcggccaac gggtgagaac gacggcgctg gaaagtgcga ctggtctcat
     8341 ccctgcggga gttgagcggt gctcctcctg ccgcgggctc tcgaagtgcg cgcgcacttc
     8401 ggggaacttc aaccggaaga gcggcgcgtc gccgtctttt ccagccggtg gccgcctgca
     8461 cttgtgcaca ggtgctcgtc gttcccctcc ggcctttacc gacatgttct gaggagaacg
     8521 ttatgaatcc caatcaggaa accatcaatc cgtccgccgc cgcggatctt caacaggcgg
     8581 ctcccacatt atcccccgag ggggtcgtcg atcagttgcg cgccacgcgg gcgcagatcg
     8641 gcgaaatgac accgttgacg gtcgcgcagc gaaaagcgct tcacgggcgc atccgtacgt
     8701 ccaatccgat tctgcaggcg tcgatcaatg tcatcggtgc gcacgacatc gtcgcgaagg
     8761 cgatcggcca gccggcagac gacatgcgtc tgttgtgcga cgagaccaac cgctggacgg
     8821 cagtggagga
//
LOCUS       DATLVW010000132         7205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_339314, whole genome shotgun sequence.
ACCESSION   DATLVW010000132 DATLVW010000000
VERSION     DATLVW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339314"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VNN25_04295"
     CDS             complement(<1..396)
                     /locus_tag="VNN25_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH90779.1"
                     /translation="MAETTLATAEVAREPKRRAYDVFLSYSSASQPWVRRFADALTSS
                     GITTWFDAHDILPGERWRDKIEKALRQSRVLIMVLTPESVQRPWTFFELGAALADGKR
                     IVPVLSEDVDPADIPALVRQFQFVREKTPE"
     gene            complement(389..820)
                     /locus_tag="VNN25_04300"
     CDS             complement(389..820)
                     /locus_tag="VNN25_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH90780.1"
                     /translation="MAKKKSKWLVFISHSSEDTWVARQIAREINDRGATPFLDEADVD
                     VGAEFEEDIRDFLDKADELFVLFTPWALERPYVWIEIGAAWQRRIPIVVVLHGLSAKE
                     FQVHPNAPVFLKKRDIIRLNEVDKYLKQLSSKVGPRKKSNG"
     gene            complement(949..3390)
                     /locus_tag="VNN25_04305"
     CDS             complement(949..3390)
                     /locus_tag="VNN25_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH90781.1"
                     /translation="MPNLPQNVDRFDVIRILGKGGMGTVYLARDQRLDRLVAVKVLHA
                     EDMGEADKRARFMREARTAAAIRHPNVATIYEVGETPEGVPFIVMEYSEGETLSQRMR
                     RRPPEAAEFLSIAKQIAAGVAAAHECGIVHRDLKSANIMIEPTGQVKILDFGLAKALP
                     TQLPRLTDSSSRRLFGTLHYLAPEQVRGQPADERADLFSVGVILYQMATGQLPFNADA
                     PLMVLEKIRDAEPEPFVARDPAFPTAATKIIGKLLKKEPHDRYQTARDLLSDLADIDT
                     PTARYAASVSPTHSSIGRTRPRPRWTRLVMTIVAFAAGAAAIVYFNRHNRAEPVPASA
                     TPPPPIRSMAVLPLDNIANNSNDDFLSVGLADALVTKLQQIPSLQVRPTSAVVEFHKK
                     KIDMKTAAEQLHVDSVLEGHFLAAGDLVRVNLQLTDSRTGYNVWADTIDGKRGDLIKL
                     IDDVSSRTVAGLNQKLGVQKAGNASEPRSSSPRAFEEYLRARALSGTLVPADHQAQEA
                     DLRRAIGFDPNFAAAYAELAIAMSLGQARGLDTAPETTERAMWYAHQAVRLDPHLAAA
                     HLALGRVFVKDPERFREAVRENLAALQLNPNDINALNNVANFFVSIGDTQRVRCVGDR
                     LVSLDPNSNEAKIRGYWYVNAVDPEGALSNAPAALAARDTELAGRDIRANAFILIGNL
                     ASAETEARRISQLVPQHYLGKSLRAMIAAAKGDRPAAEAALHSFEADANRLHWAAMRQ
                     ALCYAKLGDRNNAMHWVNRAAELGNHSWFAWIKHPWMQSLQTDPEYQTVIGKMKADLD
                     DVSGDVMGVYQLICH"
     gene            3502..>7205
                     /locus_tag="VNN25_04310"
     CDS             3502..>7205
                     /locus_tag="VNN25_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH90782.1"
                     /translation="MTSTLEALLAPDLSLPPRYQVRAILKETPSTCVYRVFDVSDQRD
                     EAIKILRHEVSEPQQLLRFKTEFSTLASLEHESIIKVFDYGLLPNSYPYFTMEYFAGK
                     KISEYFDGHSWSSLYDVILQIASALHHIHHLGIIHLDLKPSNILVDEAGKAKIMDFGV
                     AIESRQVLDRQIRGSLHYMPPEVLKQDRVDSRADLYALGMTLYETVTGALPGYGRPAI
                     EVIRMHLDEDVRPPSSINPRIPPELEQIIMKLLEKDPRQRFASAAALLQEVASAAGKK
                     VGAGELLLGGGELFAAPLIGRRAEVAQLRSLIAEARDGRGNGVIIAGAEGMGKSRIVR
                     DANLRAQLDGARVFCGRCPINRKTAYAPFFEIFQQMVLAVNPGADVSAELRRLLRPTA
                     PAGSMNDAPRATGKYQLYNRIVQAVQDMYGFLSAGASEGESNASPLILVIDDVQWADP
                     STTELFSFLVGEAKQNRLLVIGTLTIDPSTEGPSEQGTNLVAWERRAREGAFPTIRVE
                     ALNEPLVREHLQSLLGEENISDGLVRWTLWESAGSPLNIRRVVDYLIAHAYLYWTPAG
                     WIADMDRIEALRIPGGAGSILMEKVEALPPELRAVIEAAAVFGESSGVDVLTSVAGPS
                     AEEAYVALRGVVSLGLLDESNDGKMITFPQIHLREAIYNAMTERRRTELHHRIAVVLE
                     SALTAGATHILGEVAYHYARANEQIKGVHYSVEAGDLAMRTIAHEGATEFYRSALELM
                     DLAGAEEAKKAEVREKLADAYYRSSDHRAAMQAYQFLLKSIQARSKDDAPNADLARVM
                     KKIGKVLGKRGEQEAAQSYYQSAIDQYEKLGRWNDVAELLNRIAWMYRERDDIAAARA
                     SSERALALLDPSKPTVVFGYVMNTLGCLEYNLGNWQASRELLLEALKVADKVGSEQLS
                     KVATTNIGNTLWKLGDWINALQYYKMNLALSEAEGDLWDLITAYNNVGIIEFSRGNFQ
                     VAAEFFEKSVRIDEKIGAVENEALARENLAESLEALGRWNDALVQYNRCIGLQGFDET
                     RASRSSVYIPLARLMGKKGDIAKAMSYAQKALSAAERARDEDLIAEASYVLANIEDER
                     ENYGEAERHLARTLSIFASHQTTQGLSRAHTAAASMAFTQQRMDEAWRHAELGAMYAR
                     QLDDRFALAKNDWMWGKIFSSQGERDAANAKFESARATFELLETPYEHARLLFDIGLL
                     KDEPEEATQTIRSAIRIFERLEATHDLERAR"
BASE COUNT         1401 a         2152 c         2271 g         1381 t
ORIGIN      
        1 ttccggcgtc ttttcccgga caaactgaaa ctgacggaca agggcgggta tgtccgcggg
       61 gtccacatct tcagaaagca cgggaacgat gcgttttcca tccgccagag cggcacctag
      121 ttcaaagaac gtccatgggc gttgaacgct ctcgggcgta agcaccatga tcagaactct
      181 gctctggcga agcgcctttt cgattttgtc tcgccagcgc tcgccgggaa ggatgtcgtg
      241 agcatcgaac caggttgtaa tgcccgacga ggtaagcgca tccgcgaacc tgcgaaccca
      301 tggctgacta gcagaagaat acgagagaaa tacgtcgtac gcgcgccgtt ttggctcgcg
      361 cgcgacctcc gccgttgcta gtgtggtttc agccattgct cttcttcctt gggccaacct
      421 tcgagctcaa ctgcttgaga tacttgtcta cttcattcaa acgaatgatg tcccgtttct
      481 tcagaaatac aggagcattc ggatgaactt gaaactcctt tgccgacaaa ccgtgcagga
      541 cgaccacgat cggaatgcgg cgttgccatg ccgcaccgat ctcgatccag acgtacgggc
      601 gctctaatgc ccacggcgtg aaaagaacga aaagctcgtc tgccttgtcc agaaagtctc
      661 gaatatcttc ttcaaattcc gcccctacat cgacgtcggc ttcgtctaag aagggcgtcg
      721 cgccacgatc atttatctca cgtgcgatct gacgcgcaac ccatgtgtct tcgcttgagt
      781 gactgatgaa aacaagccat ttgctcttct tcttcgccat cgcatctcgt tgagcgcgtc
      841 atgttcatcg tatcgcaagc gatgaatgtt tgacactgcg acgtccaggc ggagtgccgg
      901 gcagcagcag acggcgcaga gtgcaaagga taggtttgcg gcgtgccctc agtggcagat
      961 gagctgatac acacccatca catcgcccga cacgtcatcg agatcggctt tcatctttcc
     1021 gatgacggtc tggtactcgg gatcggtctg cagcgactgc atccacgggt gtttgatcca
     1081 ggcgaaccag ctgtggtttc caagctcagc ggcgcggttg acccagtgca tggcattgtt
     1141 gcgatcaccg agttttgcgt agcagagtgc ctggcgcatc gccgcccagt gaaggcggtt
     1201 ggcgtccgct tcgaacgaat gcagcgccgc ttccgcggcc ggacgatcac ctttcgcggc
     1261 tgcgatcatc gcgcgaagac tcttgccgag gtagtgttgc gggacgagtt gggaaatgcg
     1321 gcgggcctct gtctccgcgc tggcgagatt gccgatcagg atgaacgcat tggcccggat
     1381 gtcgcggccg gccagctccg tgtcgcgtgc ggccagcgcc gcgggcgcgt tcgacagcgc
     1441 cccctccgga tcgacggcgt tgacgtacca atagccgcgg atcttggctt cgttggagtt
     1501 gggatcgagg ctgaccaggc ggtcgccgac gcatcgaacg cgctgcgtat cgccgatgga
     1561 gacgaagaag ttggcgacgt tgttgagcgc gttgatgtcg ttcggattca actgcaacgc
     1621 tgccagattc tcgcggaccg cctcgcggaa acgttccggg tccttgacga acacgcggcc
     1681 cagagcaaga tgagccgcgg caagatgcgg atcgagccgc accgcctggt gtgcgtacca
     1741 catggcacgc tcggtcgttt ccggcgcggt gtccaacccg cgagcctggc cgagagacat
     1801 ggcgatcgcg agctcggcgt aggcggcggc gaagttcgga tcgaaaccga tggcacggcg
     1861 cagatcggct tcctgtgcct gatgatccgc ggggacgaga gttccgctga gcgcgcgggc
     1921 acgcaagtac tcttcgaatg cgcgtggact cgacgaccgc ggctccgatg cgttgcctgc
     1981 cttctgcacg ccgagctttt gattcaggcc ggcgaccgtg cgcgaggaaa cgtcgtcgat
     2041 cagcttgatg agatcgccgc gcttgccgtc gatcgtgtcg gcccagacgt tgtagcccgt
     2101 tcgcgagtcg gtgagttgga gattcactcg cacgagatcg ccggcggcga ggaagtgtcc
     2161 ttccagcacg ctgtcgacgt ggagctgctc ggcggcggtc ttcatgtcga ttttcttttt
     2221 gtgaaattcg acgaccgcgg acgtcggccg cacctgcagc gacgggatct gctgcaactt
     2281 tgtcacgagc gcgtccgcca gaccgaccga gaggaagtca tcgttggagt tgttcgcgat
     2341 gttgtcgagt ggaagcaccg ccatcgaacg gatgggaggc ggcggggtgg ccgacgcagg
     2401 aacaggttcc gcccggttgt gacgattgaa gtagacgatc gccgctgcgc cggccgcgaa
     2461 cgcgacgatg gtcatgacga gccgtgtcca tcgcgggcgc gggcgcgttc gtccgatcga
     2521 cgaatgcgtc ggcgacactg aagccgcata tcgggcagtt ggtgtgtcga tgtcagcgag
     2581 gtcgctcagc aggtcgcgag cggtctgata acggtcatgc ggttcttttt tcagcagctt
     2641 gccgatgatc ttcgtggcgg cggtagggaa ggcgggatcg cgcgcgacga acggctccgg
     2701 ctcggcgtcg cgaatcttct ccagcaccat cagcggcgcg tcggcgttga acggaagctg
     2761 gcccgtcgcc atctgataga ggatgacgcc gaccgagaag agatcggcgc gttcgtcggc
     2821 cggctgacca cgcacctgct ccggcgccag atagtgaagc gttccgaaaa ggcgtctgct
     2881 cgaggaatcg gtcaaccgag gtaactgtgt cggaagggcc ttggccagac cgaaatcgag
     2941 gatcttcacc tgacccgtcg gctcgatcat gatgttcgcc gacttcaggt cgcggtgcac
     3001 aatgccgcac tcgtgcgcgg cggcgacgcc cgcggcgatc tgtttggcaa tcgagagaaa
     3061 ctcggccgcc tcgggtggcc ggcgccgcat cctctgcgac agcgtttccc cttccgagta
     3121 ctccatcacg atgaagggaa caccctcggg agtctcgcca acctcgtaga tcgtggcgac
     3181 gttgggatgc cggattgccg cggctgtcct cgcttcccgc atgaatcgcg cgcgtttgtc
     3241 cgcctcgccc atgtcctcgg cgtgcaggac ctttacggcg accagccgat cgagccgttg
     3301 atcgcgggcc aggtagaccg tccccatacc gcctttgccc aggatgcgga taacgtcgaa
     3361 tcgatcaacg ttctgaggca ggttcggcac gcggccgata ttgtacttga cggacgagtg
     3421 gtcaactttc tgacccgcaa cggaaccttg ctgggttaat atttacaccc gtaacagaat
     3481 tgtcatcttg atcaacgaaa catgaccagc acacttgaag ctctgctcgc tcccgacctc
     3541 tctcttcctc cgcggtacca agttcgcgcg attctcaaag aaacgccctc gacttgtgtg
     3601 tatcgcgtgt tcgatgtttc ggaccagagg gacgaggcga tcaagattct ccggcatgag
     3661 gtgtcggagc cgcagcaact tttaaggttc aagacagagt tttcgactct ggcctcgctc
     3721 gagcacgaga gcatcatcaa agtcttcgac tacggtctgc tgccgaattc ttacccctac
     3781 ttcaccatgg agtatttcgc gggcaagaag atctcggagt acttcgacgg acacagctgg
     3841 tcatcactct acgatgtgat tctgcagatc gcctcggcac tgcatcacat ccaccatctc
     3901 ggcatcattc acctcgatct gaagccgtcg aacatcctcg tcgatgaggc cggcaaggcc
     3961 aagatcatgg atttcggagt ggcgatcgag agccggcagg tgctggaccg gcagatccgt
     4021 ggctcgctgc actacatgcc gcccgaggtg ctgaaacagg atcgcgtgga ctcgcgggcc
     4081 gacctttacg ccctcggcat gacgctctac gagacggtca cgggcgcgct gcccggttac
     4141 ggccggccgg cgatcgaggt cattcgcatg catctcgacg aagacgtgcg tccgccttcg
     4201 tcgatcaatc cgcgaatccc cccggagctc gagcagatca tcatgaagtt gctcgagaag
     4261 gatccgcggc aacgcttcgc ctcggcagcg gcgcttctgc aggaagttgc atccgcggca
     4321 ggaaagaaag tcggtgccgg tgaattgctg ctcggcggtg gagagctctt cgcggctccg
     4381 ctcatcggcc gccgggccga ggtcgcgcag ctccggtccc tgatcgccga ggcgcgcgat
     4441 gggcgcggta acggcgtgat catcgccggc gcggagggaa tgggaaagag ccgcatcgtc
     4501 cgcgatgcca atctgcgcgc gcagctcgac ggagcgcgcg tcttctgcgg acgctgtccg
     4561 atcaaccgca agaccgcgta cgcgccgttc ttcgagatct ttcagcagat ggttctggcg
     4621 gtgaatcctg gagcagatgt ttccgccgag cttcgccgcc tgcttcgccc gacggcgcca
     4681 gccgggtcga tgaacgacgc gccgcgggcg accggcaaat accagctcta caaccgcatc
     4741 gtgcaggcgg tgcaggatat gtacggcttc ctcagcgccg gcgcctcgga aggggagtcg
     4801 aacgcatcgc cgctgatcct cgtgatcgat gacgtgcagt gggccgatcc gtcgacgact
     4861 gagcttttct cgtttctggt tggcgaggcg aaacagaatc gccttcttgt catcgggact
     4921 ctcacgatcg acccgtcgac cgaaggacca tcggagcagg gaacgaacct cgtcgcgtgg
     4981 gagcgccgcg cgcgggaggg agcgttcccg accatccgcg tcgaggcgct caatgagccg
     5041 ctcgtccgcg agcacctcca gtctctcctc ggcgaagaga acatctccga cggtcttgtg
     5101 cgctggactc tgtgggagtc tgcaggttcg cccctgaaca tccgccgcgt cgtcgactat
     5161 ctcatcgcac acgcctatct gtactggacg cccgccggat ggatcgcgga catggatcgc
     5221 atcgaagcgt tacgcattcc gggtggtgcg ggatcgatcc tgatggagaa agtggaagcg
     5281 cttcctccgg agctccgggc ggtgatcgag gccgccgcag tgttcggcga gtcttccggc
     5341 gttgacgttc tcacgtccgt cgccggtcct tcggcggaag aagcctatgt agcgttgcgc
     5401 ggcgtcgtct cgctcggtct gctcgacgag tccaacgacg gaaagatgat cacgtttccg
     5461 cagattcatt tgcgcgaggc gatctacaac gcgatgaccg agcgccgccg cacggagttg
     5521 catcaccgca ttgccgtggt gctggaatcc gcgctgacgg caggtgcgac gcacattctc
     5581 ggcgaagttg cttaccacta cgccagagcc aacgagcaga tcaaaggcgt gcactactcc
     5641 gtggaagcgg gcgacctggc aatgcgcacg atcgcgcacg agggtgcaac ggagttttat
     5701 cgcagcgcgc tcgaactgat ggatctcgcc ggcgcggagg aagcgaagaa ggcggaggtc
     5761 cgcgagaaac tggccgacgc ctactaccgc agcagcgatc accgcgcggc gatgcaggca
     5821 taccagttcc tgctgaagag cattcaggct cgcagcaaag acgacgctcc gaacgccgat
     5881 ctcgcccggg tcatgaagaa gatcgggaag gtgcttggca agcgaggcga gcaggaagca
     5941 gcgcagtcgt actaccagag cgcgatcgat caatacgaga agctcggcag atggaatgat
     6001 gtcgccgaac tgttgaaccg cattgcgtgg atgtatcgcg aacgggacga tattgcggcg
     6061 gcgcgtgcat cgtccgaacg ggcgttggca cttcttgatc cgagtaaacc gaccgtcgtc
     6121 ttcgggtacg tgatgaacac gctcggttgc ctcgaataca acctcggcaa ctggcaagcc
     6181 tcgcgcgagc tcctgctcga ggcgctgaaa gtggccgaca aggtcggttc cgagcaactc
     6241 tccaaggtgg caacgacgaa catcggcaac acactgtgga agctgggcga ttggatcaac
     6301 gccctgcagt actacaagat gaacctggcc ctttcggaag cggaaggcga tctgtgggac
     6361 ctcatcacgg cgtacaacaa cgtcgggatc atcgagttca gccgcggcaa ttttcaggtg
     6421 gcggccgaat ttttcgagaa gagcgttcgc atcgacgaga agatcggcgc ggtcgagaat
     6481 gaagcgctgg cgcgggagaa cctggcggaa tcgttggagg cgctcggccg ctggaacgac
     6541 gcgctcgtgc aatacaaccg ctgcatcggc ctgcagggct tcgacgagac gcgcgcctcc
     6601 cgttcatcgg tgtacatccc gctggcgcgg ctgatgggaa agaagggcga cattgcgaag
     6661 gcgatgtcgt acgcgcagaa ggcgctctcc gcggcggagc gcgcccgtga cgaagatctc
     6721 atcgccgaag cgtcgtacgt tctggcgaat atcgaggatg agcgcgaaaa ctacggtgag
     6781 gccgaacgcc acctggcgcg cacgttatcg atcttcgcct cgcatcagac cacgcaggga
     6841 ctctcgcgag cgcacaccgc agcggccagc atggccttca cgcagcagcg gatggatgaa
     6901 gcgtggcggc atgcggagct gggcgcaatg tacgcgcgcc aactcgacga ccgcttcgcg
     6961 ctggcaaaga acgattggat gtggggaaag atcttttcgt cgcagggtga acgtgacgcg
     7021 gcaaacgcca agttcgagtc cgcgcgcgcg acattcgagc tgctcgagac gccgtacgag
     7081 catgcacggc tccttttcga catcggcctt ctcaaggacg aaccggaaga ggcgacgcaa
     7141 acgattcgga gcgccattcg catcttcgag agactcgagg ccacgcacga tctcgagcgc
     7201 gcgcg
//
LOCUS       DATLVW010000133         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_583534, whole genome shotgun sequence.
ACCESSION   DATLVW010000133 DATLVW010000000
VERSION     DATLVW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583534"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNN25_04315"
     CDS             complement(<1..415)
                     /locus_tag="VNN25_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90783.1"
                     /translation="MKHGPGLIVVPCQELARYHRFTDSLANLEKPEGSLVQFTVGMSI
                     TDNLNMGVRSMQPEHEWVWLLGDDHIFDPNTLMRLLDRNEDIIAPLVVQRFPPFGLID
                     FQERIGDTEFHRRVQFEDLPDEPFVVERSGSLMLIR"
     gene            complement(412..978)
                     /locus_tag="VNN25_04320"
     CDS             complement(412..978)
                     /locus_tag="VNN25_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90784.1"
                     /translation="MPSRYEDRTKEELLELAADKNVEGRSSMSKDELITALRGEPSDA
                     SDEPKVPVADPATVPAAGDPPPVTEPGEEPQHPKPGEAPGPAAAVAAPHYERLSAPGQ
                     RSPSTPAEFATDWHMEAERDALIRGLAGAKGRLLEAQQLEDGAPNKEQRIKDAKAEIK
                     AVNDKLDHYGWGQKSASKRPAGQAKSTR"
     gene            complement(1039..2397)
                     /locus_tag="VNN25_04325"
     CDS             complement(1039..2397)
                     /locus_tag="VNN25_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HXH90785.1"
                     /translation="MPDDQAPDLSEKYSGLVQRYNESCEMVDNAFIAQEKAREDANTT
                     EEQRDKLLAEYSSAVTVRDRLYEEMQDKSKLEEARAQHRPITFDGKVARVKEPDVYRE
                     DNVHEISFFRDLYNAQLKNDPAAQDRINRHQQHEIEKRAVTSSTLGGIIPAQYLVDLY
                     AKASRNGRVFADQVNGQTLPDVGMSLVVPRLTQGLSAAAQTTQNTAVVTQDPTETDLT
                     VNVNTIAGYSPVSRQAIERAAYSDRILFEDLTARYWAVLDTYCINGSGSNNQPLGLLG
                     TSSISTSAASTMTAAGIYPKIADIIQQIAVAVGGLGYGATKIFMHPRRWGFFEASVDS
                     SNRPLIVPSGPGFNAMGTDNNPEADVGLVGNMHGIPVYVDPNIPTNLGTNTNEDRIIA
                     IAHPIVHLWERSNDPVTLSFEQQAGTSLQVQLVCYGYVAFTAGRYPAASGVVTGLTPP
                     TF"
     gene            complement(2412..3164)
                     /locus_tag="VNN25_04330"
     CDS             complement(2412..3164)
                     /locus_tag="VNN25_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HXH90786.1"
                     /translation="MTELRYAIAPIQDVEVRDTSGTDDNTWTMSGYAAVFDETTTLLD
                     SKFIRLTESIDQRAFNGVLANQGLNTSDGVVHFNFGHDMNRAVAATNVPAGTPGSLEL
                     STDKRGLRFMAKVPRDDPDGIALAVKMRNGVVKQASFAFTVKEASYDYRELDEGPDTE
                     HRTIMEVAHLYDVCATPQGAYSSTEAGLRSYSASLGQPSDWDGGQHRQPESGPGGETE
                     VSPETEGRGSSNDLEWPDLRESYFRHRPIKVE"
     gene            complement(3157..>3543)
                     /locus_tag="VNN25_04335"
     CDS             complement(3157..>3543)
                     /locus_tag="VNN25_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HXH90787.1"
                     /translation="GVQLERAVPNLEQDLATWLRFGLGPELERIESALEADEQLFGGA
                     QTYPGFDTQEFVRGDLQTEATILGTLVQQGIITPDEARTRLWGGLEELPDGLGKIPQV
                     TPVGGAPNPNPLPAVQPVSQNGGGYD"
BASE COUNT          532 a         1113 c         1173 g          725 t
ORIGIN      
        1 gtctgatgag catcagcgag ccggagcgct cgaccacgaa cggctcatcg ggtagatcct
       61 cgaactgaac gcgtcggtga aactccgtgt ctccgatccg ctcctgaaag tcaatcagcc
      121 cgaacggtgg aaagcgctgc acgaccagcg gcgcgatgat gtcctcgttg cggtcgagca
      181 gacgcatcag cgtgttcggg tcgaagatgt ggtcgtcacc gagcagccag acccattcgt
      241 gctcgggctg catcgagcgc acgcccatgt tcaggttgtc ggtgatcgac atgccgaccg
      301 tgaactggac gagcgagcct tccggcttct ctaggttcgc cagcgagtcg gtgaagcggt
      361 ggtaacgcgc aagctcctgg cacgggacga cgatcaggcc ggggccgtgc ttcatcgcgt
      421 cgacttcgcc tgaccggccg gccgcttgct cgcggacttt tgaccccatc cgtagtgatc
      481 gagcttgtca ttgaccgcct tgatctcggc cttggcgtcc ttgatccgct gttccttgtt
      541 cggagctccg tcctcgagtt gctgagcctc gagcagtcgc cccttggctc cggcgaggcc
      601 gcggatcagc gcgtcccgct cggcttccat gtgccaatcg gtcgcgaact cggcaggcgt
      661 cgacggtgag cgctggccag gtgccgagag ccgctcgtag tgcggcgcgg ccaccgctgc
      721 cgcaggaccg ggcgcctctc cgggcttcgg atgctgtggc tcctcacctg gctcggtcac
      781 cggaggcggg tcccctgctg ccgggactgt cgccggatcg gccaccggaa ccttcggctc
      841 gtccgaggca tcggaaggct cgccgcgtag tgcagtgatc agttcgtcct tgctcatgct
      901 cgagcgacct tcgacgttct tgtcggccgc gagctccaga agctcctctt tggttcggtc
      961 ttcgtatctg cttggcattc gaatcctcct gtccttggtg ggggccgggt gcccgacccc
     1021 cacccaaaga ctgtccgctt agaaggtcgg cggggttaga cccgtgacga ctccggaagc
     1081 ggctgggtag cggccggctg tgaaggcgac atacccgtaa cacaccagct gaacctgcaa
     1141 gctggttccg gcctgctgct cgaagctgag cgtgaccggg tcgttggagc gctcccagag
     1201 atgcacgatc gggtgcgcga tggcgatgat gcggtcctcg ttcgtgttgg tgccgaggtt
     1261 cgtcgggatg ttcgggtcga cgtagacggg gatcccgtgc atgttgccga ccaggccgac
     1321 gtcggcctcg gggttgttgt cggtgcccat ggcgttgaag ccggggcccg acgggacgat
     1381 cagcggccgg ttggacgagt cgaccgatgc ctcgaagaat ccccagcgcc gtgggtgcat
     1441 gaagatcttg gtggctccgt agccgagacc gccgaccgca accgcgatct gctggatgat
     1501 gtctgcgatc ttcgggtaga tgcccgccgc cgtcatcgtt gatgccgccg aggtcgagat
     1561 gctcgaggtg ccgagcagtc cgagcggctg gttgttcgag ccgctgccgt tgatgcagta
     1621 ggtgtcgagc actgcccagt agcgagccgt cagatcctcg aagaggatcc ggtcgctgta
     1681 tgcagcccgc tcgatcgcct gccgcgagac cggcgagtag ccggcgatcg tgttcacgtt
     1741 gaccgtcagg tcggtctcgg tcgggtcctg cgtgacgact gcggtgttct gcgtcgtctg
     1801 cgctgccgcc gagagcccct gggtcaggcg aggcacgacc agcgacatac cgacgtcggg
     1861 aagcgtctgg ccgttgacct ggtctgcgaa gacgcgcccg ttgcgggagg ccttggcgta
     1921 gaggtcgacc aggtactgcg cggggatgat cccgccgagc gtcgagctcg tgacagcgcg
     1981 cttctcgatc tcgtgctgct ggtggcggtt gatccggtcc tgcgccgccg ggtcgttctt
     2041 cagctgagcg ttgtagagat cgcggaagaa gctgatctcg tggacgttgt cctcacggta
     2101 gacgtccggc tctttgacgc gtgccacctt gccgtcgaaa gtgatcggcc ggtgctgggc
     2161 acgggcctcc tcgagcttcg acttgtcctg catctcctcg tagaggcgat cgcggacggt
     2221 gacggcggac gaatactcgg ccagaagctt gtcgcgctgc tcctcggtcg tgttcgcgtc
     2281 ctcgcgcgcc ttctcctgcg cgatgaaggc gttgtccacc atctcgcagg actcgttgta
     2341 gcgctggacg agccccgaat atttctcgga gaggtccggc gcctgatcgt caggcatgac
     2401 tgcccctttt gctactcgac cttgatcggc ctgtgcctga agtaactctc cctcaggtcc
     2461 ggccattcca ggtcgttgct gctcccgcgg ccttccgtct ccggactgac ctcggtctcg
     2521 cctcccggtc cactttcggg ctgacgatgc tggcctccgt cccaatcgga cggctgaccg
     2581 agcgaagccg agtagctccg caaacctgct tcggtgctgc tgtaggcgcc ctgaggcgta
     2641 gcgcacacgt cgtaaagatg agccacttcc atgatcgttc ggtgctcggt gtcgggacct
     2701 tcgtccagtt cccggtagtc gtagctcgct tccttgaccg tgaaggcgaa tgaggcctgc
     2761 ttgacgacgc cgtttcgcat cttcactgcc agcgcgatcc cgtcagggtc atcacgcggc
     2821 accttcgcca tgaagcgcag cccgcgcttg tcggtcgaga gctcgaggct tccaggtgtg
     2881 ccggcgggga cattggtggc agcgacggcg cgattcatgt cgtgaccgaa gttgaagtgc
     2941 acgacgccgt ccgaggtgtt gagaccctgg ttggcgagca cgccgttgaa ggcgcgctgg
     3001 tcgatcgact cggtcaggcg gatgaacttg ctgtcgagca gcgtcgtcgt ttcatcgaag
     3061 acagccgcgt agccgctcat cgtccaggtg ttgtcatcgg ttccggaggt gtcccgcacc
     3121 tcgacatcct gaatcggagc gatcgcatag cgaagctcag tcatatccac cgccattctg
     3181 acttacgggc tggacagcgg gtagcggatt cgggttcgga gcgccaccaa caggcgtgac
     3241 ctgcgggatc ttgccgagac catccggcaa ttcctcgagg ccaccccaga gccgggtgcg
     3301 cgcttcgtcg ggtgtgatga tgccctgctg gacgagcgtc ccgaggatgg tcgcttccgt
     3361 ttgcagatcg ccacggacga actcctgcgt gtcgaagccg ggataggtct gggcgcctcc
     3421 gaagagttgc tcgtccgcct cgagcgccga ttcgatccgt tcgagctcgg ggccgaggcc
     3481 gaagcgcaac caggtagcca ggtcctgctc gaggttcgga actgcgcgct ccagctgcac
     3541 tcc
//
LOCUS       DATLVW010000134        12999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_417647, whole genome shotgun sequence.
ACCESSION   DATLVW010000134 DATLVW010000000
VERSION     DATLVW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417647"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VNN25_04340"
     CDS             complement(<1..660)
                     /locus_tag="VNN25_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXH90788.1"
                     /translation="MLRIVLLFISGVAMASELPKTVAETTDYKSTSRYDDVISFVRAI
                     QSIDPGIRVETFATTNEGRALPLVIVGPAGVVDPRTAHASGLPIVFIMANIHAGEVEG
                     KEAAQMLLRELVSSKRKLREKLVILIAPIYNADGNEKISTEHRKEQVGPVNGVGVREN
                     GQGLDLNRDYMKLESPEARGLVSKVLDRWDPLVTVDLHTTDGSYHGYQLTYAPMLTPD
                     AD"
     gene            744..1187
                     /locus_tag="VNN25_04345"
     CDS             744..1187
                     /locus_tag="VNN25_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90789.1"
                     /translation="MAQNEPSMDENNRRWPKTTVDASTAKNEPSMAENEPSMDQNEPS
                     MDQNEPSMDQNEPSMDQNEPSMDQNEPSMDQNEPSMDQNEPSTDQNEPSMDQNEPSMA
                     KKSRRWIKTNHRWPKRAVDGSKRTIDGQKEPSMDQNEPSMAKKNH"
     gene            complement(1265..2032)
                     /locus_tag="VNN25_04350"
     CDS             complement(1265..2032)
                     /locus_tag="VNN25_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH90790.1"
                     /translation="MIIRDITAPNPGPFTLSGTRTYLLGASTVIDPGPAIDSHIDAIR
                     DAMPALRTILLTHRHEDHAPAAVPLKRATGARILAPAGVLDDTIVDQRIAGGESLLIE
                     SEALQVIATPGHTSEHVCFLTSAGDLFTGDTVLGEGTTTIFPPDGSMSCYIDSLRVLL
                     THKPKRIFPGHGPTRDDACAVIEQYIAHRLEREQQVLDSLAAGATSAAAIRERIYPDL
                     DHRLRGAAEIQINAHLDKLREEGRVVERGGEYKVIRK"
     gene            complement(2029..2736)
                     /locus_tag="VNN25_04355"
     CDS             complement(2029..2736)
                     /locus_tag="VNN25_04355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXH90791.1"
                     /translation="MTSHEVSNDQLPRGTGDSPEVMPLPAASVILLRDSPLQVLMMRR
                     HAKASFVPDAWVFPGGMVDASDRELGDGTEIGTMRVAAIRELFEESGIWLGAPRDDVD
                     EWRRALLAGEVTFATLVREAPLNLDALVLTSRWITPIGVPKRFDTCFFLAIAATDAVP
                     SVDNGEDGEAVEVRWITPSAAIDTLPIVFPTLKNLEALAGFDNAKQLLDSRRGIDVPT
                     TRPVLVVENGKKKIILP"
     gene            complement(2747..3163)
                     /locus_tag="VNN25_04360"
     CDS             complement(2747..3163)
                     /locus_tag="VNN25_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HXH90792.1"
                     /translation="MPFLTDDPIDSAALVRSVMRPSDGAYVLFEGVVRNHHEGKAVES
                     IFYDAYRPMAEKEIARIVDEVQHANADVAIAVVHRLGHLVIGDASIAIVAASPHRAEA
                     FAACRMIIDRIKETVPIWKKERGPGGEEWVGWQGKC"
     gene            complement(3167..3859)
                     /locus_tag="VNN25_04365"
     CDS             complement(3167..3859)
                     /locus_tag="VNN25_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90793.1"
                     /translation="MDHAVALVQAYLQLNGYFTSAEYPIIAAAGRNGFRTLTDIDVLA
                     FRFPSGLPSPDTGASRKTPRGLEMNDVDPGLGVPSDSIDMLIGEVKEGRVGINSGARD
                     PQVLKAVISRLGDSTLDRDGIVADLLAHGSATLPSGFVIRLVAFGSFPPGAEVPPCRI
                     ISLGHVLDFLQRYVRKHWNVLRHLQFKDPAFGFLMTLEKAKRGGAGRRGEAGVEIVSS
                     TPRATGPRRPGR"
     gene            4006..4365
                     /locus_tag="VNN25_04370"
     CDS             4006..4365
                     /locus_tag="VNN25_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXH90794.1"
                     /translation="MANYDCTKCPAYCCSYERIVVNKRDLKKLAKHFDLDPEVAAKRF
                     TKTREGEVVLRHQKDKIYGSVCMFLDTKTRRCTIYNARPGVCHEYPDYPTCGYYDFLS
                     WEREQQQDEKFIPLKKG"
     gene            4425..5423
                     /locus_tag="VNN25_04375"
     CDS             4425..5423
                     /locus_tag="VNN25_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH90795.1"
                     /translation="MNRLMMCTAALLLLAVPVAGQSSPPAAESAAPPGVMSETVSVGY
                     VMIPFTVLGDKGRPLKDLRDAEVDLMVDGQRVRSDMFEKSENAPVSFTILLDGSGSMA
                     LAGKMDSARAAIGALIGHRRPGDDFALYVFAASKAVEVVPFTQNVAEITNGLAKVDPY
                     GKTAFFDALVTMPERSSLGKNPSRAIILLSDGIDNASSMTRGALASRLQGISVPIYPL
                     GLRDPGARTDDRKVPREDLSDIGLLNEVATLTGGKLYLGTTPQQIAEAVSHIEDDLRA
                     QYLIGFAPTGKGAVKYRTISLKLTGRARSVHVRAGYLGTEPPQVASRNKNNERKKS"
     gene            5423..6064
                     /locus_tag="VNN25_04380"
     CDS             5423..6064
                     /locus_tag="VNN25_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXH90796.1"
                     /translation="MNQKTGLRIVTTTALAVMLGACATSKSVDQKIAEAQAQSSKKIE
                     SVEGQVEDLQTKQKATDTHLSQNDAAIAQLSQESKDALKRAQEAGVLAKGKVVFEQTF
                     KEDRIKFKVNSYELNKDAQAALDDFAGKVKALNEQYYIEIQGHTDDTGGRKLNDELGE
                     RRADEVRRYLSRSQQVPLNRMSTISYGDSLPVSSNKTRKGRAENRRVVIVVLE"
     gene            6164..6427
                     /locus_tag="VNN25_04385"
     CDS             6164..6427
                     /locus_tag="VNN25_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXH90797.1"
                     /translation="MKTATIREFRGNIAKLIDSDESVLVTKHGKPAAIMYPLRDPAGI
                     PLEVRRKLYVAMAGEIAKKLAAQNVTEEEIERDFAEFKKRRRR"
     gene            6456..6809
                     /locus_tag="VNN25_04390"
     CDS             6456..6809
                     /locus_tag="VNN25_04390"
                     /inference="COORDINATES: protein motif:HMM:NF021634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH90798.1"
                     /translation="MAALRVCRAPNLAIVTTDENVAEVLEYLPQFAARYGLPEETLLD
                     TFELLPIQVYSQPDYSTHLFAARALMAGRDPDEVPLAALALKLGIPIWSNDRDFEDFP
                     HGVYPTARLLNLLGL"
     gene            complement(6814..7560)
                     /locus_tag="VNN25_04395"
     CDS             complement(6814..7560)
                     /locus_tag="VNN25_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXH90799.1"
                     /translation="MKIATWNVNGIRARETQLLDWVASAQPDVICLQELKASPAQIPE
                     SICNLNGYWCYWHGAVAYSGVALHMRRETFPEEPKFSHPHFDLETRIVEARSGDAVFA
                     SVYVPNGGKDYVAKIDFMKALIGYARAQKEQGLKLVISGDYNIARTDMDVHPKERKPN
                     IIGQRPEERELLEAMLAEGLVDVGRKLYPEDEEYFSWWAPWRNLRQRNIGWRLDYVLA
                     SEVLAERAISCPSYREIGTSDHAPVVATFE"
     gene            complement(7756..8184)
                     /locus_tag="VNN25_04400"
     CDS             complement(7756..8184)
                     /locus_tag="VNN25_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXH90800.1"
                     /translation="MKRLSTIALLLLTACATSSSNAPNADVLLKADREFAKQSAARGH
                     LAWYEFMAPGASKPANGGRVLTGPQEISDSMLAAFGSGFTLSWEPARAQISRGGKLGY
                     TWGRYHSVFRGKARDGTYMTVWEKQPDGSWKVLFDTGDPD"
     gene            complement(8181..9227)
                     /locus_tag="VNN25_04405"
     CDS             complement(8181..9227)
                     /locus_tag="VNN25_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH90801.1"
                     /translation="MPEKTRIRPPIIELALARTKEFLREKEAMFWVFGFPLILALALG
                     FAFREKPPDRIPVGVVAGPNAQQRLTALQKSKTLLPRIMNEQEARDALRRGKVSLLIE
                     GTDTVVYRFDATRPDALSARNETDDVLQAAAGRRNVLTPREERVHEQGARYIDFLIPG
                     LLGMNLMGTGMWSMGFTIANARMKKLLKRLVATPMRKTDYLLAMFLSRLIWLLLEVSV
                     LVAFGWIVFGVRVNGSLAVLAILCIVGGYAFSGIGLLTASRAKTIEAVSGLMNLIMMP
                     MWLCSGVFFSYERFPDSVKPFIRVLPLTLLNDALRAVVNDAAGLPQISLQLGLLAVWG
                     IVTFIIGLKIFRWQ"
     gene            complement(9220..10140)
                     /locus_tag="VNN25_04410"
     CDS             complement(9220..10140)
                     /locus_tag="VNN25_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH90802.1"
                     /translation="MPDVIACRNLRKYYADVKAVDGLDLSVASGECFGLLGPNGAGKT
                     TTIEILEGLTESDSGEVEILGLTWRKNERELRERIGISLQETQLTEKLTVFETLRLFR
                     SFYRVGADPEELLRDLSLDEKHDARVGKLSGGQRQRLAVACALVGNPDVLFLDEPTTG
                     LDPQSRLQLWDIVQRFRQKGGTVLLTTHYMEEAERLCDRVAIVDHGHLIALGTPAELI
                     AKLQARNVIEFTSEPRLEQAVVEAIDGCAGVTAHREGWLLRVHSLAEAVPRLIDTVEK
                     QGAKLVTLSTHEATLEDLFVSLTGRELRDA"
     gene            10255..11214
                     /locus_tag="VNN25_04415"
     CDS             10255..11214
                     /locus_tag="VNN25_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXH90803.1"
                     /translation="MRAIVFDHFGDESVLHLGEVDPPPLGVDELRIRVRAAGVNRADL
                     LQRQGHYPPPPGASPILGMECAGEVIEVGSNVTGWRAGDRAMALLAGGGYAEETVVHH
                     GSAMHVPDFLSDDEAGGIPEVFLTAYLNVFELARARTGETLLVHGGGSGVGTAATTLA
                     KLAGLRVIVTAGSREKCERCLAHGADEAINYNEDDFVERARGANVILDHIGARYLARD
                     LECLAVGGRVVVIGSMGGPGSIDLNVGSLLSKRQQIIGSTLRARPNEEKAAIVASFVA
                     RFGDDLRAGRIRPVIDRIFPIAEAAAAHRALKEDHFGKIVLKM"
     gene            11257..11466
                     /locus_tag="VNN25_04420"
     CDS             11257..11466
                     /locus_tag="VNN25_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH90804.1"
                     /translation="MSRAKLSATYRVRIPKEVRESAHLKQGQEFQIIVKGGIVHLVPV
                     KSLEEMRGFAPGLTTEGLRDKSDRM"
     gene            11463..11840
                     /locus_tag="VNN25_04425"
     CDS             11463..11840
                     /locus_tag="VNN25_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH90805.1"
                     /translation="MNVIDSCGWIEVFAGGPLSDLFISYLRDLHSVITPTIVVYEVYK
                     KLKQTTSEDDALSALTILQQTRVVPLSVGISLTAADLAIKYGLSMADAIVLATGYESD
                     ADIVTSDRAFENLPRVVYYAKRG"
     gene            complement(11847..12491)
                     /locus_tag="VNN25_04430"
     CDS             complement(11847..12491)
                     /locus_tag="VNN25_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXH90806.1"
                     /translation="MRRLAILILNVMVTLACHLGPTSPATSAASANDLAKTKRLLDAG
                     LDPNARDDAGYTPLIAASRAGKVPMIRLLRSHGADPNLRDAAVNSWTPLLHAIHKAQP
                     ASVAALLDAGADPNGTDKDGTTPLMMAAGYGYTDIVKLLLARGANPRIATSDGITALD
                     LARAGVPDIDRFTVFACQDDTVEALLAADPKLPDHTANLPLPARIAMHAKRCKA"
     gene            12609..12767
                     /locus_tag="VNN25_04435"
     CDS             12609..12767
                     /locus_tag="VNN25_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90807.1"
                     /translation="MSDQKAHDLLRKHNISLDDVKQRRTTLHHLRHIVHSEELPFDVF
                     KALVKLRR"
BASE COUNT         2668 a         3849 c         3997 g         2485 t
ORIGIN      
        1 gtcagcgtca ggtgtcagca tcggggcgta ggtgagttgg tagccgtggt acgagccgtc
       61 ggtggtgtgc agatcgacgg tgacgagcgg gtcccaccgg tcgagcacct tgctcacgag
      121 cccgcgcgct tcgggggatt cgagcttcat gtagtcgcga ttgaggtcga gcccctgccc
      181 gttctcgcga acgcccacgc cattcaccgg ccccacctgt tccttgcgat gctcggtgga
      241 gatcttctcg tttccgtcgg cgttgtaaat cggtgcgatg aggatgacga gcttctcgcg
      301 cagtttgcgc ttcgaggaaa cgagctcgcg caacagcatt tgcgccgcct ctttcccttc
      361 cacctcgccc gcatggatgt ttgccatgat gaagacgatc ggcaatccgc tggcgtgggc
      421 agtgcgcgga tcgacaacgc ccgctggtcc aacgatgaca agcggcagcg cccgtccttc
      481 gttcgtcgtc gcaaaggtct cgacccggat gccaggatcg atcgattgga ttgcgcggac
      541 gaaggagata acgtcgtcgt atcgcgacgt tgatttgtag tccgtcgtct ccgcaaccgt
      601 cttcggcaat tcggaggcca tcgcaacccc gctgatgaaa agaaggacga ttcgcaacat
      661 cagtcgcatg aaggaactct accagcggct ggactggcac agaggatcaa gaagcacggt
      721 cgacggttca aaaagcaccg tcgatggctc aaaacgaacc atcgatggat gaaaacaacc
      781 gtcgatggcc gaaaacaacc gtcgacgcgt cgacggctaa aaatgaacca tcgatggccg
      841 aaaacgaacc gtcgatggat caaaatgagc catcgatgga tcaaaacgaa ccgtcgatgg
      901 atcaaaacga gccatcgatg gatcaaaacg agccatcgat ggatcaaaac gaaccgtcga
      961 tggatcaaaa tgagccgtcg atggatcaaa acgaaccgtc gacggatcaa aatgaaccat
     1021 cgatggatca aaacgaaccg tcgatggcca agaagagccg tcgatggatc aaaacgaacc
     1081 atcgatggcc aaaaagagcc gtcgatggat caaaacgaac catcgatggc caaaaagaac
     1141 cgtcgatgga tcaaaacgaa ccatcgatgg ccaaaaagaa ccattgatgg ctcaagacat
     1201 accgcgatgg cgtgagaaca gctcctggcg ggcgtgaaat acagctccgc ggttgcttcc
     1261 gtgctcattt gcgaatgact ttgtattcgc caccgcgctc aacgacccgc ccttcctccc
     1321 tcagcttgtc caggtgtgcg ttgatctgaa tctccgctgc gccgcggagg cggtgatcga
     1381 gatcgggata gatccgttcg cgaatcgcgg ctgccgaggt tgcgccggcg gcgaggctgt
     1441 cgaggacttg ctgctcgcgc tcgaggcggt gagcgatgta ctgctcgatc acggcgcagg
     1501 cgtcgtcgcg ggtcggaccg tgtcccggaa agatgcgctt cggtttgtgg gtaagaagga
     1561 cgcggagcga atcgatgtaa cagctcatcg atccgtcggg tgggaagatc gtggttgttc
     1621 cttcgccgag gaccgtgtcg ccggtgaaga gatcgccggc ggacgtgaga aagcagacgt
     1681 gctcgctcgt gtggccgggc gtggcgatga cctgtagcgc ttcactctcg atgaggagac
     1741 tctcgccgcc tgcgatgcgc tgatcgacga tcgtgtcatc gagaacacct gccggagcga
     1801 ggattcgcgc acccgtagcg cgtttgagcg ggactgcggc aggcgcgtgg tcttcgtggc
     1861 gatgcgtgag caggatcgtg cgcagcgccg gcatcgcatc gcggatggca tcaatgtgcg
     1921 agtcgatcgc cgggccggga tcgatcacag ttgatgcgcc gagcaggtac gtgcgcgtgc
     1981 cgctcagcgt gaacggtccg ggattcggcg cagtgatgtc gcgaatgatc acggcaggat
     2041 gatcttcttc ttcccgttct cgacgacgag caccggccgg gttgtcggga cgtcgattcc
     2101 gcgccgtgag tcgagcagtt gtttcgcatt gtcgaatccg gcgagcgctt cgagattttt
     2161 cagcgtggga aaaacgatcg gcagcgtgtc gatcgccgcg gacggtgtga tccagcggac
     2221 ttcgacggct tcgccatctt cgccgttgtc gaccgaagga acagcgtcgg tggcggcgat
     2281 agcgagaaag aagcaggtgt cgaaacgctt cggaacgccg atgggtgtga tccatcgcga
     2341 agtcaaaacg agtgcgtcga gattgagcgg agcttcgcgg acaagcgtgg caaacgtcac
     2401 ctccccggcg agcaacgcgc gacgccattc atcaacgtca tcgcgcggcg cgccaagcca
     2461 gatgccgctc tcttcgaaca gctcgcgaat cgcggcgaca cgcatcgtgc cgatttcggt
     2521 tccatcgccg agctcgcggt cgctcgcgtc gaccatgccg ccgggaaaga cccacgcgtc
     2581 cggtacgaac gacgccttcg cgtgacgccg catcattagt acctgtagcg gcgaatcgcg
     2641 aagaaggatc acgctcgctg ccggaagcgg catcacctcg ggcgaatcgc cggtaccgcg
     2701 cggaagttga tcgttggata cttcgtgaga cgtcacgcca aatccctcag cacttgccct
     2761 gccatcccac ccactcttcg ccgccggggc cgcgttcttt cttccagatc ggaacggtct
     2821 ctttgatgcg gtcgatgatc atgcggcagg ccgcaaacgc ctcggcacga tgcggcgagg
     2881 cggccacgat agcgatcgac gcatcgccaa ttacgaggtg gccgagccga tggacgacgg
     2941 cgatcgcgac atccgcgttc gcgtgctgca cctcatcgac gattcgtgcg atctctttct
     3001 ctgccatcgg gcgataagcg tcgtaaaaaa tcgactcgac cgcttttcct tcgtggtggt
     3061 tgcgaacgac tccttcgaac agcacgtacg cgccatcgga cggacgcatc acgctgcgca
     3121 cgagcgccgc ggagtcgatc gggtcgtcag tgagaaaagg cagtggctac ctcccgggcc
     3181 gcctcggccc cgtcgctcgc ggtgtcgacg aaacgatctc taccccagcc tcccctcggc
     3241 ggcccgcacc gccacgcttc gccttctcca gcgtcatcag aaaaccgaac gcgggatcct
     3301 tgaactgaag atgccgtagc acgttccaat gttttcgcac gtagcgctgc aggaagtcga
     3361 gcacgtgtcc gaggctgatg atgcggcagg gaggaacctc ggcgcccgga ggaaacgagc
     3421 cgaaggcgac cagacggatc acgaagccgg aagggagcgt cgccgacccg tgcgccaaca
     3481 gatccgcgac gatgccatcg cgatcgagcg tcgaatcacc gagacggctg atcacagcct
     3541 tcaaaacctg aggatcgcgc gcgccgctgt tgattccgac ccgcccttct ttcacctcgc
     3601 cgatcagcat gtcgatcgag tccgaaggaa cgccgagtcc gggatcgacg tcgttcatct
     3661 cgagtccgcg cggcgtcttc cgtgaggcgc ccgtgtcagg cgatgggagg ccggacggaa
     3721 aacgaaacgc aagcacgtcg atgtccgtca gcgtccggaa tccgttcctc ccagccgcag
     3781 cgatgatcgg atattcagcc gacgtgaagt aaccgttgag ctgcaaatac gcctgcacca
     3841 gagcgactgc gtggtccatg aatgcatcat agtgaaagca gagatcagag gagcagagat
     3901 cagagacaac ggagattggg accgaggagc aagcgtcgct atcaccccct gatctctgat
     3961 ctccgcttcc ctgatctctg tccattcccg tacactcgaa tgcgcatggc gaactacgac
     4021 tgcacgaagt gcccggcgta ctgctgcagc tacgaacgga tcgtcgtcaa caagcgcgat
     4081 ctcaaaaagt tggcgaagca cttcgatctc gatcccgaag tggcggccaa gcgcttcacg
     4141 aaaactcgtg agggggaagt agtccttcgc caccaaaagg acaagatcta cggcagtgtt
     4201 tgtatgtttc ttgatacgaa aacgcgccga tgcaccatct acaacgcgcg gccgggcgta
     4261 tgccacgagt accccgatta ccccacgtgc ggctactacg attttctcag ttgggagcga
     4321 gagcagcagc aggacgagaa gttcattccg ctgaaaaagg gatagcgagc tctgcaccac
     4381 cctttccgaa atgcagattg gatagttatt tgttcacaaa accgatgaac aggctcatga
     4441 tgtgcacggc ggcgttgctg ctgctcgcgg ttcccgtcgc tgggcagtcc agcccacctg
     4501 cggcagagag cgcagcaccg ccgggcgtga tgtccgagac ggtgtcagtc ggatacgtga
     4561 tgattccctt cacggtcctc ggcgacaagg gccgtccgct caaagacctg cgcgatgccg
     4621 aagtcgatct gatggtcgat gggcaacgcg tgcggagcga catgttcgag aagtcagaga
     4681 acgcgccggt ctcgttcacg atcctgctcg acggctccgg ttcgatggct ctcgcgggaa
     4741 agatggactc ggcgcgggcg gcgatcggcg cgctcatcgg acatcgccgg cctggcgacg
     4801 acttcgcgtt gtacgtcttc gcggcatcaa aggcggtcga agtcgttccg tttactcaga
     4861 atgtcgccga gattaccaac ggtctggcga aggtcgaccc gtacggcaag acggctttct
     4921 tcgacgcgct ggtgacgatg ccggagcgga gtagcctggg caagaatccg tcgcgggcga
     4981 tcatcctgct cagcgatgga atcgacaacg cttcgtcgat gacgcgcgga gcgcttgctt
     5041 cgcgtcttca gggaatctct gttccgatct atccgctcgg ccttcgcgat cccggcgcca
     5101 ggaccgacga tcgcaaagtt cctcgcgaag atctctcgga catcggcttg ctgaacgaag
     5161 tcgccacgct gaccggcgga aagttatacc tcggaactac gcctcaacag atcgcggagg
     5221 ccgtctcgca catcgaagac gatcttcgcg cgcagtacct catcgggttc gcgcccacag
     5281 ggaagggtgc ggtaaagtac cggacgatct cactcaagct gacaggccgc gcacgctccg
     5341 tgcacgtacg cgccggatac ctcggtaccg aacctcccca ggtcgcatcc cgcaacaaaa
     5401 acaacgaaag gaagaagagt tgatgaacca gaaaacagga ctccgcatcg tcaccacgac
     5461 ggcactcgcc gtcatgctcg gcgcctgcgc cacctcaaaa tcagtcgatc agaagatcgc
     5521 tgaggcgcag gcacagtcga gcaagaagat cgaatcggtc gagggtcagg tcgaggatct
     5581 gcagaccaaa cagaaggcta ccgacacgca cctctcacag aacgacgccg ccatcgcgca
     5641 gctcagccag gaatcgaagg acgcgctcaa acgcgctcag gaggctggtg tcctggcgaa
     5701 agggaaggtc gtcttcgaac agacgttcaa ggaagaccgg atcaagttca aggtcaactc
     5761 ctatgagctg aacaaggacg cccaagcggc actcgacgac tttgccggca aagtgaaggc
     5821 actgaacgag cagtactaca tcgagattca gggccacacc gacgacaccg gcggacgcaa
     5881 gctcaatgac gagttgggcg aacgccgtgc cgacgaagtg cgccgctacc tctcccgctc
     5941 gcagcaggtg ccactgaacc gcatgtcgac gatctcgtac ggcgattcgc tgccagtgtc
     6001 atcgaacaag acgaggaagg gccgcgccga gaaccggcga gtcgtcatcg tcgtgctcga
     6061 gtagtttcta tttcgcggat gagaacaggg cggactttcg ggtccgcctt ttttgcgtcg
     6121 ctggaagttg tgtacacact cgtgtatctt atttggccac gccatgaaga cggcaacgat
     6181 tcgcgaattc cgaggcaaca ttgcaaaact gatcgacagc gacgaatccg tgctcgttac
     6241 gaagcacggc aaacccgcgg cgatcatgta tcccctgaga gacccagcag gaatcccgct
     6301 ggaagttcga cggaagctgt acgtggcaat ggctggggag atcgcgaaga aactcgctgc
     6361 ccagaatgtc accgaagaag agatcgagcg tgacttcgca gaatttaaga agcgtcgccg
     6421 ccgatagcaa cgttctcctt tccgcgattt ccgggatggc cgcgcttcgg gtatgcagag
     6481 cgccgaatct cgcgattgtg acgacagacg agaatgtcgc cgaggtgctc gagtacttgc
     6541 cgcagttcgc tgcccgatac ggacttcccg aagagaccct cctcgacacg ttcgagttgt
     6601 tgcctattca agtctattca caacctgact actcgactca tctcttcgcc gctcgcgctt
     6661 tgatggcggg ccgcgatccg gacgaagtcc cgctggcagc tctggcgttg aaacttggta
     6721 taccgatttg gtccaacgac cgcgacttcg aagattttcc gcacggcgtc tacccgactg
     6781 caaggctgct gaatcttctc gggttgtgac gatctactcg aacgtcgcca ccaccggcgc
     6841 gtggtcgctt gttccgatct cgcgatagga cggacatgag atcgcgcgct cggccagcac
     6901 ttcactggcc agcacgtagt cgaggcgcca gccgatgttg cgctgacgga gattgcgcca
     6961 tggagcccac caactgaagt actcctcatc ctcgggatac agcttccttc cgacatcgac
     7021 gaggccctcg gccagcatcg cttcgagtaa ctctcgctct tcggggcgct gaccgatgat
     7081 gttcggcttg cgttctttgg gatggacgtc catgtcggtg cgggcgatgt tgtagtcgcc
     7141 gctgatgacc agcttcagtc cctgttcttt ctgtgcgcgg gcatagccga tgagcgcttt
     7201 catgaagtcg atcttggcga cgtagtcctt gccgccgttc gggacgtaga cagatgcgaa
     7261 gaccgcgtcg ccggagcgcg cttcgacgat gcgggtctcg aggtcaaagt gcggatgcga
     7321 gaacttcggc tcctcgggaa acgtttcgcg ccgcatatgc agcgcaacgc cggagtacgc
     7381 aaccgcgcca tgccagtagc accagtagcc attgagattg cagatcgact cggggatctg
     7441 cgcaggcgag gctttgagct cctgtagaca gatcacatca ggttgcgcgc tcgcgaccca
     7501 atcgagcagt tgcgtctcgc gggcgcggat tccgttcacg ttccaagtgg cgattttcat
     7561 ggtttgaggg tgtgggggtg gggagtggga tgtcggagca aaagcgaaac catccgacgc
     7621 ttcgctcaaa gccctcgacc tcttgttccc cgcttcctat tctcccagtt cagctatcga
     7681 gtcgttgtgt cgaattttcg gacaggaacg cttcgcgtct tctcccacca cccactccct
     7741 actccccact ccccatcagt caggatctcc cgtatcgaac agcactttcc atgaaccgtc
     7801 aggctgcttt tcccacacgg tcatgtatgt gccgtcgcgt gctttgcctc ggaagaccga
     7861 gtggtatcga ccccacgtgt agccgagctt tcccccgcgc gagatttgcg ctcgggccgg
     7921 ctcccacgac agcgtgaagc cgcttccgaa tgcagcaagc atgctgtcgg agatttcctg
     7981 tgggccggtc agcacgcgac cgccgttggc cggcttggag gcacccggcg ccatgaattc
     8041 gtaccaggca agatggccgc gggctgccga ctgtttcgcg aactcgcggt cggccttcag
     8101 caaaacatcg gcgttcggcg cgtttgacga cgacgtggcg catgccgtca gaagcagcag
     8161 ggcgatcgtt gagagtcgtt tcattgccat cgaaagatct tcaacccgat gatgaaggtg
     8221 acgatgcccc acacggccag caatccgagc tggagcgaga tctgaggcag gccggcggca
     8281 tcattgacga ccgcgcgaag cgcatcgttc agcaacgtca gcggcaggac gcggatgaat
     8341 ggcttgaccg aatcggggaa tcgttcgtac gagaagaaaa cgccggagca gagccacatc
     8401 ggcatcatga tcaggttcat caggccgctc accgcttcga tcgtcttcgc ccgcgatgcc
     8461 gtcaacagcc caatgcctga gaacgcgtag ccgccgacga tgcaaagaat ggccagcact
     8521 gcgagcgagc cattgacgcg cacgccgaac acgatccagc cgaaggcgac cagcaccgaa
     8581 acctcgagaa gaagccagat gagccgcgac agaaacatcg ccagcaggta gtccgtcttg
     8641 cgcatcggcg tggccacgag acgcttgagc agcttcttca tgcgcgcgtt cgcgatcgtg
     8701 aagcccatgc tccacatccc ggtgcccatg aggttcatgc cgaggagacc ggggatcaga
     8761 aaatcgatgt agcgagcgcc ctgctcgtgg acgcgctcct cgcgcggcgt tagaacgttg
     8821 cgacgtccgg ccgcagcctg gagcacatcg tccgtctcgt tgcgcgcgct cagtgcatcg
     8881 ggacgtgtgg catcgaagcg atagacgacg gtgtccgttc cctcgatcag cagcgagacc
     8941 ttgccgcgcc gcagggcgtc gcgtgcttcc tgctcattca tgatgcgcgg cagcagcgtt
     9001 ttcgatttct gtaacgctgt cagccgctgt tgcgcgttcg gtccggccac gacaccgacc
     9061 ggtatgcgat cgggcggctt ttcgcgaaag gcgaatccca gagcgagggc caggatcagc
     9121 ggaaagccga agacccagaa catcgcttcc ttctcgcgca gaaactcctt cgtgcgggcc
     9181 agcgccagct cgatgatcgg aggccggatg cgcgttttct caggcatcgc gcagctccct
     9241 tccggtcagc gagacaaaga gatcttcgag cgtcgcttca tgcgtcgaca gggtcacgag
     9301 cttcgccccc tgtttctcga ccgtgtcgat caaccgcggc actgcctcgg cgagcgaatg
     9361 aacgcgcagc agccaaccct cgcgatgcgc ggtgacgccg gcgcagccgt cgatggcttc
     9421 gacgacagcc tgctcgagcc gcggctcgct cgtgaactcg atcacgttgc gcgcctgaag
     9481 ctttgcgatc aactcggctg gcgtgccgag ggcgatgagg tgaccgtggt ctacgatggc
     9541 gactcggtcg cagaggcgtt ccgcttcttc catgtagtgc gtagtcagaa gaaccgtccc
     9601 acccttctgc cggaagcgtt gcacgatgtc ccacaactgc aggcggctct gcggatcgag
     9661 gccggtcgtc ggctcgtcga gaaagagaac atcgggattg ccgaccagcg cgcatgccac
     9721 cgcgagccgc tgccgctgac cgcccgagag cttgccgacg cgtgcgtcgt gtttctcatc
     9781 gagcgagaga tcgcgtagca actcctcggg atcggctccg acgcgataaa acgatcgaaa
     9841 caaccgcagc gtctcgaaga cggtgagctt ctcggtgagc tgcgtctcct gcagcgagat
     9901 gccgatgcgc tcacgcaact cgcgctcgtt tttgcgccat gtgagaccga ggatctcgac
     9961 ctcgccggag tcggactcgg tgagaccttc gaggatctca atcgtcgtgg tcttgcccgc
    10021 tccattcggc ccgagaagtc cgaaacattc gccactggcg accgagaggt cgagtccgtc
    10081 gaccgccttc acgtcggcgt agtacttgcg caaattgcgg caggcgatga cgtcgggcat
    10141 gcggagggaa acgatacaac aagatttgac gcgcgcccga gggagagaat ggcagcgtac
    10201 acatgcagaa atcgttcgac gcctcttggc taccgttgtg acgaggagga gaaaatgcga
    10261 gcaatcgttt tcgatcactt cggcgatgaa tccgttcttc acctcggcga agtcgatccg
    10321 cctccgctcg gcgtggacga gctgcgcatt cgcgtgcgtg ccgcgggagt gaatcgcgcc
    10381 gatctgctgc agcgccaagg ccactatccg cctcctcccg gcgcctcgcc aatcctcgga
    10441 atggaatgcg cgggcgaagt gatcgaagtc ggttcgaatg tcacaggttg gagggccggc
    10501 gaccgtgcga tggcgttgct cgcgggtggc ggttacgccg aggagacggt cgttcatcac
    10561 ggatcggcga tgcatgtgcc ggatttcttg agcgatgacg aggctggcgg catcccggag
    10621 gttttcctca ccgcctatct caacgtcttc gagctcgcgc gtgcacgcac gggcgagacg
    10681 ctcctcgttc acggcggcgg cagcggcgtc ggcacagccg cgaccacgct cgcgaagctg
    10741 gcaggattgc gcgtgatcgt taccgccggc tcgcgcgaaa agtgcgagcg ctgtctcgcg
    10801 cacggcgccg acgaagccat caattacaac gaggatgact tcgtcgagcg ggcacgcggc
    10861 gcgaatgtga ttctcgatca catcggcgcg cgatacctcg ctcgcgatct cgaatgcctc
    10921 gcagtcggtg gccgtgtcgt cgtcatcgga tcgatgggcg gcccgggcag catcgatctg
    10981 aacgtcggat cgctcctcag caagcgccag caaatcatcg gatcgacatt gcgcgcccgg
    11041 ccgaacgagg agaaagcggc gatcgtggcg tcattcgtcg cgcgtttcgg tgacgacctg
    11101 cgcgcgggcc ggatccgccc ggtgatcgat cgcatctttc cgatcgccga ggccgctgcg
    11161 gcgcatcgcg cgttgaagga agatcacttc gggaagatcg tgctgaagat gtgacgttgc
    11221 cgttgacgtg ttattttgtg gcacgaaagg tcgaatatga gccgcgcaaa attgtcagcg
    11281 acataccggg tgaggattcc gaaggaagtg cgtgagtccg cgcatcttaa acagggacag
    11341 gaatttcaga tcatcgtaaa gggcggcatt gttcatctgg tgccggtaaa gagtcttgag
    11401 gagatgcgcg gtttcgcccc tggcctgacg accgaagggc tgcgcgacaa gagcgaccgg
    11461 atgtgaacgt catcgattcg tgcggatgga tcgaggtgtt cgcaggcggg cctttgtcgg
    11521 atttgttcat atcctacctg cgcgaccttc attcggttat cacgccgacg atcgttgttt
    11581 atgaggtcta caagaaactg aagcagacaa cgtcggagga tgatgctctg tcggcgctca
    11641 cgattcttca gcaaacaaga gtcgttccgc tttccgtcgg aatctcgctt acggctgcgg
    11701 acctcgcgat taagtacggt ctctcgatgg cagatgcgat tgtgctcgcc acaggctacg
    11761 agtcagacgc ggacatcgtg accagtgata gggcgttcga gaacctgccg cgggttgtct
    11821 actacgccaa gcgggggtaa ctcgcgttat gccttgcacc gcttcgcgtg catcgcgatt
    11881 cgcgccggca acggcaggtt tgcggtgtgg tcggggagct ttggatcggc ggcgagaagt
    11941 gcctccaccg tatcgtcctg acaagcgaag acggtgaagc gatcgatgtc ggggacgccg
    12001 gcgcgcgcga ggtcgagagc ggtgatgccg tcgctcgtgg cgattcgcgg gttggctccg
    12061 cgcgcgagga gaagtttcac gatgtcggtg tagccgtagc cagcggccat catcaatggt
    12121 gtcgtgccgt ccttgtcggt tccattggga tcggcgccgg catcgagcag agcggcgacc
    12181 gacgcgggtt gcgctttgtg aatcgcgtga agcaacggcg tccaagagtt gactgcagcg
    12241 tcgcggaggt tcggatcggc tccgtggctg cgcagaagcc ggatcatggg cacctttccg
    12301 gcccgggatg cggcgattag aggggtgtag ccagcgtcat cgcgagcgtt tggatcgagt
    12361 ccggcgtcga gcaagcgctt cgtctttgcc aggtcgttcg cgctcgcggc cgatgtggcg
    12421 ggcgaggtcg gaccgaggtg gcaggccagc gtgaccatga cgttgaggat gagaatggca
    12481 aggcgtcgca tgaacgcaat gtaggatcga aatcgccttg agtcaagtac ttgtcactgt
    12541 caagtgaaat agctttcgga ttggacctgt tcgactgagc tccgaatcaa ccatttgtgg
    12601 ggagagctat gtctgaccag aaagcacacg atcttcttcg taagcacaac atctcgctcg
    12661 acgacgtgaa acaacgccgg acgacgctgc accaccttcg ccacatcgtt cactccgagg
    12721 agctgccgtt cgacgtcttc aaggcgttgg tcaagctgcg ccggtaagcg gggatgtggg
    12781 ttggatctgt ggagatcaga gactagagat cagtggatta ctaaatcaga agacgaacgc
    12841 tgcgtggcat tctgatctct tgtctcttgt ctcttgtctc ttgtctctga tctctgatcc
    12901 ccgatcacga atcgactttc cttttccgcg ccacgagaac gaatgccgcg ccgtagagcg
    12961 ccatcgcgat cgcggtcaga aaacgcagtc cggtgacga
//
LOCUS       DATLVW010000135         8118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_583608, whole genome shotgun sequence.
ACCESSION   DATLVW010000135 DATLVW010000000
VERSION     DATLVW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583608"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..536
                     /locus_tag="VNN25_04440"
     CDS             153..536
                     /locus_tag="VNN25_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXH90808.1"
                     /translation="MSDYYQDLIAWQKAMVLAECAYELTEAFPKREMYGLARQVRNAA
                     VSVPANIAEGHGRITRGEWQQFLGIARGSLLEVETELILAARFKYINQSQLKIALSKS
                     SEVGRLINGLLRASGSRAPRKPFGG"
     gene            823..1038
                     /locus_tag="VNN25_04445"
     CDS             823..1038
                     /locus_tag="VNN25_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90809.1"
                     /translation="MEIPSKVSIFSRSLELKGKPGTLIAINDNGFYEIVMDFQGRNHV
                     VLFPIAETAVIFNEALPTIGADFEVER"
     gene            complement(1096..2094)
                     /locus_tag="VNN25_04450"
     CDS             complement(1096..2094)
                     /locus_tag="VNN25_04450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXH90810.1"
                     /translation="MKKATILMILALAAPAVFAQNKAAAPTTPAPATPAASAQAGDPV
                     VVSASGVTIHKSEFEAALKTLPAEYQSYAQGPGKKQFADDYLRMRLLANEGMKSGLEN
                     DPEVQNQLTLMRENLVASAELKKIDSGIAVSDADVQKAYADNAKQYEQVKARHILIAP
                     KGSPAAQTGKKELTDDEAKAKAEDLRKQIVAGANFEELAKKESDDAGSGARGGDLGAF
                     GHGQMVPEFEAAAFAAKVGEVTPVVKTQFGYHIIKVDAHDSKPLAEVRPEIEKNLKQV
                     KLHAALDAMKDSAHPVYDEKYFAPPPAPEAPAAVPVPPPAPKSDTKKDAVTPKKKP"
     gene            complement(2387..3637)
                     /locus_tag="VNN25_04455"
     CDS             complement(2387..3637)
                     /locus_tag="VNN25_04455"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXH90811.1"
                     /translation="MAGVAYDARGAILVMEMNVITRPDIRFTRRVREMQISPTLAVMN
                     RAIELTAQGVDVVDLGPGEPDFPTPESVCAAGKRAIDQGLTKYTSGNGTKALRDALAG
                     RYNRRYGTSLAAENVIAGTGGKQELFNITLALVDEGDEVIIPSPYWVSFPDQVLFAGG
                     KPVFAKTDARNGFRPTLEDIEAVASERTRGVIINSPNNPTGAVITEQELRRIVEWCAS
                     RDAFLMFDETYEFFVYEGSKHVSAVRWFEEYPSTIIGVNSMSKTFAMTGWRLGYAVAH
                     RDVIAAAGKIQSHSTSNPSSISQAAALEALGGGEDEVQRMWEAYHERRAWLIPAINAI
                     DGLRCDNTDGAFYIFPDVSSFFGGRIRDSQSFADYLLDEARVAVIPGSAFGSDDHVRI
                     SYATSMERLKEGVGRIASALSKLR"
     gene            complement(3595..4773)
                     /locus_tag="VNN25_04460"
     CDS             complement(3595..4773)
                     /locus_tag="VNN25_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90812.1"
                     /translation="MILKLIKTSTAASAAAWSALWTFPAILLSSFIIAWAAEAAQFLM
                     AQGLALAILAWLQTLPEFAVEAVIAWQAGKDPSKTHLVIANFTGSLRLLVGLGWPMIY
                     FVAAAFGWKREHRFITVELDAEHSIEVFGLFLPIAYFTFIWWKGSLTIWDALPLIASY
                     VLYLWILWKIPPRDDEGEALEDLGFVPRRVLMMPPRMRNAAILTLFFAGGGILFFAAH
                     PFLDSMLAIATSLGVSTFIFVQWVAPFLSEFPEKLSAFYWARKVRTANIALMNMVSSN
                     INQWSILSAMIPIIFVFSAGELRPLTFDVHQRAEILLTILQSFIGFLLLMNLQLKMHE
                     ALILFVLWLAQFAMPPWRGPMIYVYLAWCAVELVRLAVSGHAPPAWRGLRTTLAARFS
                     "
     gene            4803..5492
                     /locus_tag="VNN25_04465"
     CDS             4803..5492
                     /locus_tag="VNN25_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXH90813.1"
                     /translation="MTISSRQNVWFKRIREAIHDHDDEIVIEGPKAVADAVAAGWVPI
                     KTFGRGVDFTAGLFDSLAETKSPQNVIGLFHRPQSSLDQVFARDASVVIALDGVQDPG
                     NVGTIVRLAAAFDANGIVLLPGCADPYSPKAIRSSAGAILSVPVVSASVEELLTRGWP
                     IVAADARGKAGDPPARRAVLAFGSEGAGLSPVLAEHARTIAIRMSTRIESLNVATSAA
                     ILLARSYELRA"
     gene            5606..6427
                     /gene="rsmI"
                     /locus_tag="VNN25_04470"
     CDS             5606..6427
                     /gene="rsmI"
                     /locus_tag="VNN25_04470"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXH90814.1"
                     /translation="MPGKLLIVGTPLGNLGDFPPRAIEALKSADLILCEDTRHTRKLL
                     THFGINQPSERYDDHTEEAKSGAFAERIAAGETIALVSDAGMPVVSDPGFRLVRLARE
                     RGLTIEPIPGPFAGVLALVASGIAPLPFTFLGFAPHRTGERTDFYRDMAKLGHTAIIY
                     ESPERVVGSLEDALAVLGDVEVTVAREMTKLHEEILSGTISEVLAELRGRQSIYGEIT
                     IVFAAALKAAASTSPEEVAAEFERLRAGGMRRNDAVKAVAEKFGMRKNDVYRMLI"
     gene            6643..7791
                     /locus_tag="VNN25_04475"
     CDS             6643..7791
                     /locus_tag="VNN25_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXH90815.1"
                     /translation="MDFRLTDEQTQIQEMVRDFALAEIKPHLPEWDEAQHFPVDTFKR
                     AGGLGMLGVTIPEEYGGAGLSYIDYIHVVEELAVIDSGFALSVAAHNSLCTGHIYLAG
                     NDEQKKKWLPKLTSGEWIGAWGLTEPSSGSDASGMHTTAVKDGDDWVLNGTKNFITNA
                     TYADISVVLAVTDRNDSKRGVTAFAVERARPGIRAGKKENKLGMRVSDTAELVMEDCR
                     VPASNILGEIGHGFIGAMKVLDGGRISIGALAVGIARGAYEAARDYAKQRHAFGKSIA
                     EFQAIQFMLADMATEIDAARLLCYRAATMKDAGEKVTQASAMAKLYASEVAVRATEKG
                     VQIFGGYGFIKEFPAEKFYRDVKLCTIGEGTSEIQRMVIARGLLAESS"
     gene            7897..>8118
                     /locus_tag="VNN25_04480"
     CDS             7897..>8118
                     /locus_tag="VNN25_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90816.1"
                     /translation="MADHSDSRPPIPDSREDSQPPTSDSLAHALTSGKYRALAQAVSL
                     VERDDGRAERLLADIYPATGRARIIGITGS"
BASE COUNT         1552 a         2560 c         2447 g         1559 t
ORIGIN      
        1 atctgatctc tgatccgatt cccgtctgat ctctatcctg cttcccgatc tctgatctct
       61 gattatctct gatctccagc aggcttcatg tagactcata atcccacacg ggcggccgct
      121 cggcatgctc gctccctagg caccggcaaa tcatgtcgga ctattaccag gatctcatcg
      181 catggcagaa ggctatggta ttggcggagt gcgcctacga actgactgag gcgtttccga
      241 agcgcgagat gtacggtctc gcacgtcaag tgcgtaacgc tgcggtctcc gttccggcca
      301 acatcgcaga ggggcacggc aggatcaccc gcggagaatg gcagcagttc ctcggcatcg
      361 cgcgcggatc gctgctcgaa gttgagaccg aattgattct cgccgcacgc ttcaaataca
      421 tcaaccagtc tcaactcaag attgccctga gcaaatcgtc agaggtggga aggttgatca
      481 acggattgct ccgcgcgagc ggcagcagag cgccccggaa gccgttcggg ggctgagccg
      541 cggtgggaca gagatcagtg agacagagat cagagatcag acgctggtct ctggtctctc
      601 gtctctggtc tctcgtctct ggtctctcgt ctctggtctc tcgtctctgg tctctcgtct
      661 ctgatctctc gtctcttgtc tggccgctga tctccctggc tttgatctat ttgtctgcga
      721 tgcctgatcc ttgatctctg atctctctat ctctgatctc ttcgtctctg atctctgctg
      781 tcccgttgac tccatccctc gcgcaaccta cccttacacg caatggaaat tccttcgaag
      841 gtctcgatct tcagccgcag cctcgagttg aagggcaagc caggcactct catcgccatc
      901 aatgacaacg gcttctacga gatcgtcatg gattttcagg gccgcaacca tgtcgtgctc
      961 tttccgatcg ccgagactgc cgtcatattc aacgaggcgc ttcctaccat cggcgcggac
     1021 ttcgaagtcg agcgctgaaa cgaaaacgcc gctggagacc cagcggcgtt tcagtttgat
     1081 tcggatgaat gtgccttacg gcttcttctt cggagtcaca gcgtccttct tcgtgtcgct
     1141 cttcggagcg gggggcggga cgggcacggc ggccggggct tcgggtgccg gaggaggcgc
     1201 gaagtacttc tcgtcgtaca ccgggtgggc gctgtccttc atcgcgtcga gcgcggcgtg
     1261 gagcttgacc tgcttgaggt tcttctcgat ttccggacga acctcggcca gcggcttcga
     1321 gtcgtgtgca tcaactttga tgatgtgata gccgaactgc gtcttcacga ccggcgtgac
     1381 ctcaccgacc ttcgcggcaa acgcggccgc ttcgaactcc ggaaccatct ggccgtggcc
     1441 gaatgctccg aggtcgccgc cgcgggcacc ggagccagcg tcgtccgatt ccttcttcgc
     1501 gagctcctcg aagttcgcac cggcgacgat ctgcttgcgg agatcctcgg cctttgcttt
     1561 cgcttcgtcg tcggtcagct ctttcttgcc cgtttgtgcg gccgggcttc ccttcggcgc
     1621 gatgaggatg tgccgcgcct tgacctgctc gtactgtttc gcgttgtcgg cgtacgcctt
     1681 ctgaacgtcg gcgtcgctga ccgcaatacc gctgtcgatc ttcttcaact ccgcgctggc
     1741 gacgagattc tcgcgcatca gcgtgagctg gttttggact tcgggatcgt tctccagccc
     1801 gctcttcata ccctcattgg cgagcagacg catgcgcagg taatcgtcag caaactgctt
     1861 cttgccgggt ccctgcgcgt agctctggta ttcggcggga agcgttttga gtgccgcctc
     1921 gaactccgac ttgtgaatcg tgacaccgct cgccgagacc accacaggat ctccggcctg
     1981 agccgaagcc gcaggagtcg ccggagccgg cgtcgtcggt gcggccgctt tgttctgcgc
     2041 gaacactgcc ggagcagcga gcgcgagaat catcagaatc gttgctttct tcacagaatc
     2101 tcctttgttg ccttattgcg cgatcatttg gacagcgaat cgtaacggga gggtgtgagg
     2161 cttgattcca gcgggagaca gagatcagag acaagagatc agagacagaa ggcgccgcgg
     2221 gagacggaga ttagagacaa gagatcggag acagaatgcg aagcgaccga gacaggagac
     2281 aaagagatca gagatggagc cgttgacgga gacgagaggc aagagagttc aacagtaggt
     2341 gctgtcgacc tgatctctga tctctgaatc tctgatctct gatttttcag cgcagcttgc
     2401 tcaacgccga cgcgatccgt cccacgcctt ccttcagccg ctccatcgac gttgcgtaac
     2461 tgatgcgcac gtggtcgtcg ctgccaaacg cggaaccagg aatcacggca acgcgcgctt
     2521 cgtcgagcag atagtcggcg aagctctgcg aatcgcgaat gcggccgcca aagaaactgc
     2581 tcacgtcggg gaagatgtag aaagcgccat ccgtgttgtc acagcgcagt ccgtcgatcg
     2641 cattgatggc cggaatcagc cacgcgcgcc gctcgtgata ggcctcccac atgcgctgca
     2701 cttcgtcctc gccgccgccc aatgcctcga gcgccgcagc ctgcgagatc gagctcggat
     2761 tcgatgtcga gtgactctgg atcttcccgg ccgcggcgat cacgtcgcga tgcgcaacgg
     2821 cgtagcccaa tcgccagccg gtcatggcga atgtcttcga catcgagttc acgccgatga
     2881 tcgtcgacgg atactcttcg aaccagcgca ccgcggacac gtgcttcgat ccctcgtaga
     2941 cgaagaactc gtacgtctcg tcgaacatga ggaacgcgtc gcgcgaggcg caccactcga
     3001 cgatgcggcg cagctcctgc tcggtgatga ccgcgcccgt cggattgttg ggcgagttga
     3061 tgatgacgcc gcgggttcgc tcgctggcca ctgcttcgat gtcttccagc gtcggacgga
     3121 agccgttgcg cgcatcggtc ttggcgaaaa ccggttttcc gccagcgaat agaacctggt
     3181 ctgggaagct cacccagtac ggcgaaggaa tgatgacctc atcgccttcg tcgaccaatg
     3241 ccagcgtgat gttgaacagc tcctgtttcc cgcccgtgcc tgcgatcacg ttctctgccg
     3301 ccagcgacgt gccgtaacgc cggttgtagc ggccggcaag cgcatcgcga agcgctttcg
     3361 ttccgttgcc gctcgtgtac ttcgttagtc cctgatcgat ggcgcgcttg ccggcggcgc
     3421 agaccgactc cggcgtcgga aagtcgggct caccgggccc gagatcgacg acgtcgactc
     3481 cctgcgcggt caactcgatg gcgcggttca tcaccgcgag cgtcggcgag atctgcatct
     3541 cgcgcacgcg gcgcgtgaag cggatatcag gtcgtgtgat cacgttcatt tccatcacga
     3601 gaatcgcgcc gcgagcgtcg tacgcaaccc ccgccatgcc ggcggagcgt ggccgctgac
     3661 ggccaggcgc accagctcga cggcgcacca cgcaagatac acataaatca tcggtccgcg
     3721 ccatggcggc atcgcgaatt gcgctaacca caggacgaag agaatgagcg cttcgtgcat
     3781 cttcaattgc aggttcatca gaagaagaaa gccgataaag ctctgcagaa tcgtgaggag
     3841 gatctcagcg cgctggtgta catcgaacgt cagcggccgc aactcgccgg cgctgaacac
     3901 gaagatgatc gggatcatcg ccgagagaat gctccactga ttgatgttgg acgagaccat
     3961 gttcatcagc gcgatgttgg ccgtccgaac cttgcgcgcc cagtagaacg cgctcagttt
     4021 ttccgggaac tcggagagga acggcgcaac ccactgcacg aaaatgaacg ttgagacgcc
     4081 gagtgacgtc gcgatggcca gcatcgagtc gaggaacggg tgcgcggcga agaagagaat
     4141 cccgccgccg gcgaagaaga gcgtcaggat cgcagcgttg cgcatcctcg gcggcatcat
     4201 cagtacgcgc cgcgggacga atccgagatc ctcgagcgcc tccccctcgt catcgcgcgg
     4261 tgggatcttc cacaggatcc agagatagag aacatacgaa gcgatgagcg gcagcgcatc
     4321 ccaaatcgtc agcgacccct tccaccagat gaatgtgaag taggcgatcg gcaggaaaag
     4381 cccgaagacc tcgatggaat gctcggcgtc caactcgaca gtgatgaagc ggtgctcgcg
     4441 cttccagcca aacgccgcgg cgacgaagta gatcatcggc cagcccagtc cgaccagcag
     4501 ccgcagactg ccggtgaagt tcgcgatcac gaggtgcgtc ttcgacgggt ccttcccggc
     4561 ctgccaggcg ataaccgctt cgacggcgaa ctccggcaac gtctgcaacc acgccaggat
     4621 cgccagcgcc aatccctgcg ccatcagaaa ctgcgccgcc tcggcagccc aggcaataat
     4681 gaacgacgaa agaagaatgg ccgggaacgt ccagagcgcc gaccatgccg cggcggaggc
     4741 agcggtcgac gtcttgatga gtttcagtat catcgcggag ccaggagcgc gcattctagt
     4801 tgatgaccat cagcagcagg cagaacgtat ggttcaagcg gatacgcgaa gccattcatg
     4861 accacgacga tgagatcgtg atcgaaggac cgaaagccgt ggccgacgcg gtcgcggcgg
     4921 gatgggtgcc gatcaagacg ttcgggcgcg gcgtcgattt cactgccgga ctttttgatt
     4981 cgctcgcgga aacgaaatcg cctcagaacg tcataggcct ctttcatcgg ccgcaatcga
     5041 gtctcgatca agtcttcgcc cgcgacgctt cggtcgtcat cgcgctggac ggtgttcagg
     5101 atccaggcaa tgtcggaacg atcgtgcgat tggctgccgc gtttgacgcg aacgggatcg
     5161 tgctgctgcc cggctgcgcc gatccataca gtccgaaggc gatccgctcg tcagccggcg
     5221 cgatcctcag tgtgcccgtt gtcagcgcct cggtcgagga gctgctgacg cgcggatggc
     5281 cgatcgttgc ggccgatgcg cgtggtaagg ctggcgatcc tccggcgcgg cgtgcggtgc
     5341 tggcgtttgg gagcgaaggt gctgggctct cgcctgtcct ggctgagcac gcgcgaacga
     5401 ttgccattcg gatgtcgacg cggattgagt cgctgaatgt ggcgacttcg gcggcgattc
     5461 tgctggctag gagttacgag ctgcgggcgt aggttgtgtt caacgtcgcc gccgggccgg
     5521 cgccggtaca gccggcgagc cgccggcgct acgcctttcg ctgcgtccag ccttggcgct
     5581 acggcgcgcg atacactcgc tcctcatgcc gggcaaactt ctcatcgtcg gcacgccgct
     5641 cggcaacctc ggcgattttc cgccgcgcgc gatcgaggcg ctcaaatctg cggacctcat
     5701 cctctgcgaa gacacgcggc atacacgcaa gttgctgacg cacttcggca tcaatcagcc
     5761 gtccgagagg tacgacgatc acaccgaaga ggccaagtcc ggagcgttcg ctgagcgtat
     5821 cgcagccgga gaaacgatcg cgctcgtctc cgacgccggc atgccggtcg tcagcgatcc
     5881 cggcttccgc ctcgtccgcc tggcgcgcga gcgcggactg acgatcgagc caattcccgg
     5941 cccgttcgca ggcgtgctcg cgctcgtcgc cagcggcatc gcacctctcc ccttcacatt
     6001 cctcggcttc gccccgcacc gcaccggcga gcgcaccgat ttctaccgcg acatggcgaa
     6061 gctcggccac accgcgatca tctacgaatc gccggaacgc gtcgtcggct cgctcgagga
     6121 cgcactcgcc gttctcggcg acgtcgaagt caccgtcgca cgcgagatga cgaagctgca
     6181 cgaggagatc ctcagcggca cgatcagcga agtgctcgcc gagttgcgcg ggaggcagtc
     6241 gatctacggc gagatcacga tcgttttcgc ggcagcgctg aaagccgccg ccagcacgtc
     6301 accagaggag gtcgcggccg agttcgagcg cctgcgggct ggagggatgc ggcgcaacga
     6361 tgcggtcaaa gcggtagcgg agaagttcgg gatgcggaag aacgatgttt acaggatgct
     6421 gatttaggtt tgccaaagcg aaggcgacca cagagacaca gagagcacag agatatctct
     6481 gtgacctctg tgtctctgtg atgaacggtt tttcaaattg accggccacc gctcatccga
     6541 tgctaccagc acctctctcc ctgctatcat ccctgcttcc gcatgtgcgg cgcgtgaaag
     6601 cctgcgcgcc cccgcgacaa tcagatttcc ccaggagccc gaatggattt ccgcctcacc
     6661 gatgagcaga cgcagattca agagatggtg cgcgatttcg cgctcgccga gatcaagcct
     6721 caccttccgg agtgggatga ggcgcagcat tttcccgttg acaccttcaa gcgcgccggc
     6781 ggcctcggca tgctcggcgt caccatcccc gaggagtacg gcggcgctgg tctttcgtac
     6841 atcgattaca tccacgtcgt cgaagagctc gccgtcatcg acagcggttt cgcgctcagc
     6901 gtggctgcgc acaactcgct ttgcacggga catatctacc tcgccggcaa cgacgagcag
     6961 aaaaagaagt ggcttcccaa actgacttcc ggcgaatgga tcggcgcgtg gggactcacc
     7021 gagccaagct ccggcagcga cgcctcgggc atgcacacca ccgcggtgaa ggacggtgac
     7081 gactgggtgc tgaacggcac gaagaacttc atcaccaacg ccacctacgc cgacatttcc
     7141 gtcgtgctcg ccgtcaccga ccgcaacgac tcgaaacgcg gcgtgaccgc gttcgctgtc
     7201 gagagggcgc ggccgggcat ccgagcgggc aagaaggaaa acaaactcgg catgcgcgtt
     7261 tccgacaccg ccgagctggt gatggaagat tgccgcgtcc ccgcctccaa catcctcggc
     7321 gagatcgggc acggattcat cggcgcgatg aaggtgctcg acggcggccg catctccatc
     7381 ggcgcactcg ccgtcggcat cgcccgcggc gcgtatgaag cagcgcgcga ctacgccaaa
     7441 cagcgccacg ccttcggaaa gtcgatcgct gagtttcagg ccatccagtt catgctcgcc
     7501 gacatggcca ccgagatcga cgccgcgcgc ctgctttgtt atcgcgccgc cacgatgaaa
     7561 gatgccggcg agaaagtgac gcaggcctcc gcgatggcca agctgtacgc atcggaagtc
     7621 gccgtccgcg ccactgaaaa aggtgtccag atcttcggcg gctacggctt catcaaagag
     7681 ttcccagccg aaaagttcta ccgcgacgtg aagctctgca ccatcggcga aggcactagc
     7741 gagatccagc ggatggtgat cgcgcggggt ctgctggcgg agagttcgtg agttcttacc
     7801 gggtgtcggg tgtcgggtgt cgggagccgg agctgtcaac gctcatgctt cgcgtttcaa
     7861 tcttcacgcg ccaggcctcg gcgtaaaagg tctccgatgg ccgatcactc cgactcccga
     7921 cccccgatac ccgactcccg cgaagactcc caacccccga cttccgactc cctcgcgcac
     7981 gctctcacct ccggcaaata ccgcgcgctg gcgcaggccg tatcgctcgt cgagcgtgac
     8041 gacggccgcg cggagcgatt gctggcggac atctaccccg cgaccggccg cgcgcgcatc
     8101 atcggtatca ccggctct
//
LOCUS       DATLVW010000136         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_294616, whole genome shotgun sequence.
ACCESSION   DATLVW010000136 DATLVW010000000
VERSION     DATLVW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294616"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="VNN25_04485"
     CDS             complement(<1..1906)
                     /locus_tag="VNN25_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90817.1"
                     /translation="MALFNRALLLQRLELRDEAGQIAFRAATASVGTPWQQEALDLVA
                     VRRTNDDSRTWDRERPRLESAALANDDRRVQDIVTRFPEQARKWGVSIYPTQWADDVL
                     LGSDAASRDLSTARTIGRVLQTINQDSLVADAVHSIDDSKDRATTYEIAGAYLIYRDG
                     RMALSKDRTSDAERLLRDAEAQFRTLRSPMVLAASYYRGVATHASGQTAAAEEILRPV
                     REELKRRGYRSLNAETGWELGLCLLERGDISGAETVFAESRDTFDLLGERQMRASMDG
                     LLANAAEVAGDPEQAWRYRVKALAVQTIPRRRIGMLAVAINGCIQRHEWTRASALLNL
                     TVDAAEHDRDAIEVVTALLQRSTVLAYEHDAAGARHDLAEIARWRRSPEVISIASRMQ
                     AGADFVEGLLERTDRANVAIAAFGRAASYYAHHHLEIYAPAAYLERARVERRVGRIGN
                     SALDVNAGLAVAEQIRTSVRDVQQRAMNLATMNELFDEGLTIAYERNTKNEAFELAEH
                     ARARSILESFSGDGDAPNALPLSIDAIRSAMAPDSAIIEYASLDEFTIAFVVTPSLFE
                     MARLPVTRDDIRTLSAATGRGAMSDPFERAHKMLIQPLTAWLTGVHQLAIVPDGNLFA
                     LPFASLGTANG"
     gene            2909..3286
                     /locus_tag="VNN25_04490"
     CDS             2909..3286
                     /locus_tag="VNN25_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90818.1"
                     /translation="MIRLLFAAAVGLFVLSLPLTKTKLGGQLRIAAGVCFALALVPSL
                     LFGLFFQPIPAPLGHPDPATIGTRIGAGLSCLGALVILSLFAYAILALRKKLFRPKKD
                     PWDIFFARGGGKKRVQPKDPVAP"
     gene            3283..>3901
                     /locus_tag="VNN25_04495"
     CDS             3283..>3901
                     /locus_tag="VNN25_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXH90819.1"
                     /translation="MKGVYDLLFGDRKTSYTRARANTSAASTSASDTLASLDGKDSFP
                     FGSTLHGDAVRLAPDGLGHGLTCGASGSGKSSALSLIVEGFAAAPGHRVEIIDPKGET
                     CIVLAEHAAMTVLRLPQDQRRTFAERFHVIDVRDDAVTPANLFAVPPQMSPALLAALR
                     ASAMVNAGDHEFSDLMQHGLFLVFSVAIALQRGITKKFVRRLLLDD"
BASE COUNT          714 a         1213 c         1183 g          791 t
ORIGIN      
        1 gtccgttcgc ggtgccaagg ctggcgaaag gtaacgcgaa gagattgcca tcggggacga
       61 ttgcgagctg gtgcacgccc gtcaaccatg ccgttaaggg ttggatcaac atcttgtgtg
      121 cacgctcgaa cggatcgctc atcgctcccc tgccggtcgc cgccgataaa gtgcggatat
      181 catcgcgcgt gactgggagc cgtgccattt cgaaaagtga aggcgtcacg acgaaggcaa
      241 tggtgaactc gtcgagcgac gcgtattcaa tgatggcgga atcaggcgcc attgcgctgc
      301 ggattgcgtc gattgatagc ggcaatgcat tcggagcatc gccgtcgccg ctgaatgact
      361 ccagaatgct tcgcgcgcgg gcgtgctccg cgagctcgaa tgcttcgttc ttggtattgc
      421 gctcatatgc gattgtcagt ccttcgtcga agagctcgtt catcgttgcc aaattcatgg
      481 cgcgctgttg aacgtcacga acactcgtcc ggatttgctc ggcaacagcg aggccggcat
      541 ttacgtccag cgcgctgttg ccgatccgcc cgacccgacg ctcgacacgg gcgcgctcga
      601 gatacgccgc cggtgcatag atctcgagat gatggtgcgc ataatagctg gcagcgcggc
      661 cgaacgccgc gatcgcgacg tttgcacgat cggttcgctc caataaaccc tcgacgaaat
      721 cagctccggc ttgcattctc gaagcgattg aaattacctc aggagatctc cgccacctag
      781 cgatttcggc gagatcgtga cgcgcgcccg ccgcgtcgtg ctcataagcg aggacggtgc
      841 tgcgctgcag cagtgccgtg acaacctcga tggcatcgcg gtcatgttcc gctgcgtcaa
      901 ccgtgaggtt gagaagcgcc gatgcgcgtg tccattcgtg gcgctggatg catccgttga
      961 ttgccacggc aagcattccg atacggcggc ggggtatcgt ttgcactgcg agagccttca
     1021 cgcggtaacg ccacgcttgc tcgggatcac cggcgacctc cgcggcgttt gcgagaagcc
     1081 cgtccatcga cgctcgcatc tggcgctcgc caagcaagtc gaaggtgtcg cgagattcag
     1141 cgaacaccgt ctcagcgccg gagatgtcgc cgcgctcgag aagacacaag ccaagttccc
     1201 atccggtctc ggcgttcaac gagcgatagc cgcgacgttt cagttcctcg cgcaccggcc
     1261 tgaggatttc ctccgcggcg gcggtttggc ccgatgcgtg agtcgcaacg cccctgtagt
     1321 aagaagcagc gagcaccatc gggctgcgca acgtacgaaa ttgcgcttcc gcatcgcgga
     1381 gcaacctctc agcgtccgac gttcgatcct tactcagcgc cattcgtcca tcgcgataga
     1441 tcagatatgc gcccgcgatt tcgtaggtcg tggctctgtc cttcgaatca tcgatgctgt
     1501 gaaccgcgtc tgcgacgagc gagtcttggt tgatggtttg cagaacacgt ccgatcgtgc
     1561 gcgcggtgga cagatcacga cttgcagcat cactccccag caagacatca tcggcccact
     1621 gtgtcgggta aatcgaaaca ccccactttc tcgcctgctc agggaatcgg gtgacgatgt
     1681 cctgaacgcg gcgatcatca ttcgcgagtg ccgcactttc gaggcgcggg cgctcacgat
     1741 cccaggttcg cgagtcgtca ttcgttcgcc gcacggcgac aagatcgagt gcctcttgtt
     1801 gccagggcgt gcccaccgat gccgttgctg cacgaaatgc gatttgccct gcctcgtccc
     1861 gcagctcgag ccgctgcaac aggagcgcgc ggttaaagag tgccactgca ttattcggat
     1921 cagccgatag cgcgcgatcg ctagcggcga gaccgtcgat cgccgatgcc agtttctctg
     1981 gccgttctga ctgcaacaga tacgcggcag cgagctccga gcacacgtcc aaggacccag
     2041 cccggcaaag cggagcgagt ctctcgattg cgccgtccgc gtcgttcagg agaagcttgg
     2101 ccaagccgac tgcgcgtggt gccttgtcac cgttcgccgc ggcaaccgtg cgggcctcgc
     2161 tttggaattc gagttgcgcg gtgccagcga ccggtgctcc gcgcgagatc aacagcggca
     2221 catgttcaaa cggttcgccg aggcgcgccc gaagcagtcg gctcggaagt ctggacgctg
     2281 caactgaaag tcggttgatg agtacgcgat ccaagtctct tgtgcgatcg aagatctctt
     2341 tggaggtaac cgcggcaatc gctacggcga tggccacaac accgacaact cctggcgctc
     2401 tcttcagtgc aatcgaaatc acgtaaacgc tcttttaggt aggacggacc accgagtatt
     2461 gttccagact tctgcttcgc caaggagaca tttcggttgt ggcggctgaa ttcgaccagc
     2521 gcgcaccgga ttagcaagca gtccatgagt agagttccag tcggacaacg caccgttgcg
     2581 catggtgctc gagcgcagtg ccagcggcgc gcacggggat gcatgaggtc atggtgttcc
     2641 ttgacgccgg acacgcgtgg tgtgggcccc cggccgagtt gcccggaccc gcgggcaact
     2701 ccatctttgg ggttgatcag gatcgagttg cacggacgcg tagttctgga tgagattcta
     2761 ctcacgtatc cattcgatgg gcgcccctga tctccgccaa gtccctgaaa caaccctggc
     2821 tggggcgagt ggacgattat cgaaccgctc tgctcggcct gctggcccag cccacacctg
     2881 aattctctca gattctggag gcggcgcgat gatccgcctc ctcttcgccg ctgccgtggg
     2941 cctgtttgtc ctgagcctcc cgcttaccaa gacgaagctc ggcggacagc tgcgcatcgc
     3001 cgcgggtgtc tgcttcgctc tcgcactggt cccgtctctc ctgttcggtc tcttctttca
     3061 gcccataccc gcaccattgg gtcatcctga tcccgcgacg atcggaacgc ggattggggc
     3121 gggcctgtcc tgcctcggcg cgctcgtcat cctgtcgctc ttcgcgtacg cgatcctcgc
     3181 tcttcgaaag aagctctttc gcccgaagaa agatccctgg gacatcttct tcgcccgcgg
     3241 tggcgggaag aaacgggtcc aaccaaaaga tccggtggct ccgtgaaggg cgtctacgat
     3301 ctgctgttcg gcgatcggaa gacctcctat acacgcgcac gagcgaatac gtctgcggcc
     3361 agcacctctg cgtcagacac actcgcgagc ctcgacggga aagacagctt ccccttcgga
     3421 tcgacgctgc atggagatgc cgtgcgtctc gcgcccgatg gcctcggcca cggccttacc
     3481 tgcggcgcga gcgggagcgg aaagtcgtcc gcgctgtcac tcatcgtcga agggttcgcg
     3541 gcggcaccgg gacatcgggt ggagattatc gaccccaagg gagagacctg catcgtcctg
     3601 gccgagcatg cggcgatgac ggtcctgcgc ctgccgcagg atcagcgccg aacgttcgcc
     3661 gagcgctttc atgtcatcga tgttcgcgac gatgccgtga cgcccgcgaa cttgtttgct
     3721 gtcccaccgc agatgagtcc cgcgcttctc gcggcgctgc gcgcatcggc gatggtgaac
     3781 gcgggcgacc atgagttcag cgatttgatg cagcacgggt tgttcctcgt gttctcggtt
     3841 gcgatcgcac tgcaacgcgg catcacgaag aagtttgtgc gacgactgct gttggacgac
     3901 g
//
LOCUS       DATLVW010000137         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_173376, whole genome shotgun sequence.
ACCESSION   DATLVW010000137 DATLVW010000000
VERSION     DATLVW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173376"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VNN25_04500"
     CDS             <1..289
                     /locus_tag="VNN25_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXH90820.1"
                     /translation="RAASYLTEEGLAVTKDVVLSDDIATAILDYSGRMRADIIALATS
                     GIGGMSRFVFGTVADEVTRNATTSLLVFHPRTPIEVANTNAHASTKALAGV"
     gene            291..851
                     /locus_tag="VNN25_04505"
     CDS             291..851
                     /locus_tag="VNN25_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXH90821.1"
                     /translation="MNDTKAPRAGTAATAMDVPREAPEMERMWSARHGDVIFREMSRD
                     EIESMLLRNRVGRLAFSLHDRVDIQPIHYIYERGWLYGRTSEGDKITALKHNQWVAFE
                     LDEVTDLFDWRSIVIHGSFWIMHPRGSPRAEELWTKAAELVSKIVPGALTETDPVAFR
                     QTLFRIAVSDVRGREAKMKPLEPNDP"
     gene            complement(856..2448)
                     /locus_tag="VNN25_04510"
     CDS             complement(856..2448)
                     /locus_tag="VNN25_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH90822.1"
                     /translation="MADRITHDILAEIVEIAADAIICMDAFQRITFFNKGAEAIFGWT
                     SVEIVGQRIETLIPERYRSIHPHHVAEFGRSQVTARRMGERREIAGVRKNGEEFPAEA
                     AIAQVHQGENVVYSVVLRDVTVRKRFEQRQQFLAEAGEKLASAFGSSETLNQVARLAV
                     PSMADGCILENRVGNGFLAGAAAHVDPAIEELLDEIGLAGARVPPKGHPLTEILGKPV
                     PVLLQSEAASSLLEASANLAYIKAVRAMNPQSALFLPLIAREQLIGALTLFRTSRGFD
                     GDDLGFAEDLARLAALALDNARLHDTVRASLRSRDEMLGVVSHDLRNPVAAVKMLSRT
                     LLGAQENDGTQAHESIALISQAAEQMDALIRDLLDVNRLDSGNLSISAVPVEPSVLLT
                     DSLQTLRPLVEEKGIDLDIQIETALPKVAADRERIQQTLSNLVGNAIKFSPTGSKIVV
                     VARSDSEGVVFSVLDRGRGIAEGQLPRVFDRYWQSSRTDRQGAGLGLAIAKGIVEAHG
                     GRIWIESRLGEGTTASFTLPLA"
     gene            complement(2561..>2740)
                     /locus_tag="VNN25_04515"
     CDS             complement(2561..>2740)
                     /locus_tag="VNN25_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90823.1"
                     /translation="SFALGDCASVLSIEHAARALPAIAVTAVFRRKSRREPFLANSAT
                     GIFTWSFVDMELPLE"
BASE COUNT          587 a          835 c          801 g          517 t
ORIGIN      
        1 gcgcgcggcg agctacctga cggaggaggg actcgctgtg accaaggacg tcgtgctaag
       61 cgacgacatc gccactgcca tactcgacta ttcggggcgc atgcgagctg acatcatcgc
      121 tttggccacc agcgggattg gcggcatgag ccggttcgtc ttcgggacgg ttgcagacga
      181 ggtaacgagg aacgcgacga cttcgcttct cgtctttcat cccaggaccc caatcgaggt
      241 tgccaatacc aacgcacacg cgagcacaaa agccctggct ggagtctgac atgaatgaca
      301 cgaaggcacc gcgggccgga acagccgcga cagcgatgga tgtgccgagg gaggctccag
      361 aaatggagcg gatgtggtca gcgcgtcacg gagatgtgat tttccgcgaa atgtcgcgtg
      421 atgaaatcga gtcgatgctg ctccgcaatc gcgtcggtcg tctcgccttc tcactccacg
      481 acagggtcga catccagccc attcattaca tctacgagcg gggctggctg tacggccgca
      541 cttcagaggg cgacaagatc acggcgctga aacacaatca atgggtcgcg ttcgagctgg
      601 acgaggtcac cgaccttttc gattggcgga gcattgtcat ccacggaagc ttctggatca
      661 tgcacccccg cggaagtccg cgtgccgagg aactatggac caaggcagcc gagctggtga
      721 gcaagatcgt cccgggcgcg ttgaccgaaa cagacccggt tgcgttcaga cagacgcttt
      781 tccgaatcgc cgtcagcgac gtgcgcggga gagaagcgaa gatgaaaccg ttggagccca
      841 atgacccgtg atcggtcagg ccaatggaag tgtgaatgag gcggtagttc cttcgcctag
      901 ccggctctcg atccagatcc tgccgccatg ggcctcgacg atgcctttcg cgatcgcgag
      961 gccgaggccc gcaccctgtc gatccgtccg tgaggactgc cagtaacgat caaaaactct
     1021 cggcagctgc ccctcggcga ttccacggcc acgatccagc accgaaaaga ccacaccttc
     1081 ggagtcgctt cgcgcaacta ccacaatctt cgaaccagtc ggtgaaaact tgatcgcatt
     1141 tccaacgaga ttcgacagcg tctgctgaat tcgctcgcga tccgcggcga ctttgggcaa
     1201 agccgtttct atctggatgt cgaggtcgat gcccttttct tcgacgagcg gccgaagagt
     1261 ctgcagcgaa tcagtgagga gaaccgaggg ctccactggt acagcggaga tggagagatt
     1321 tcccgagtcg agccggttca catcgagaag atccctgatg agcgcatcca tctgctcagc
     1381 tgcctgagaa atcaacgcga tgctttcgtg cgcctgcgtt ccgtcattct cctgtgcgcc
     1441 gagcaaggtg cgactcagca tcttgacggc ggcgacaggg tttctgagat cgtgagagac
     1501 gacaccaagc atctcgtcac gagaacgcag gctggcgcgc acggtgtcat gcagacgcgc
     1561 gttatccaaa gccagcgccg ccagccgcgc gagatcttct gcgaacccga gatcgtcgcc
     1621 atcgaagccg cgactcgtgc gaaaaagggt tagcgcaccg atcagttgct cacgcgcaat
     1681 gagcggaagg aagagggcgg attgcggatt catcgctctt accgccttga tgtacgcgag
     1741 gttggcgctc gcttccagaa ggctggacgc cgcttcggac tgcaacaaga caggaactgg
     1801 ctttccgaga atctctgtca gcggatgccc tttgggcgga acacgcgcgc cagccaggcc
     1861 gatctcatcg agcagttcct caatggccgg atcgacgtga gccgcggcgc ctgccaggaa
     1921 cccatttcca actctgtttt ccaggatgca gccgtcagcc atggaaggaa ccgccaaccg
     1981 cgccacctga ttcaacgtct ccgatgatcc gaatgcagac gccagcttct cgcccgcctc
     2041 ggcaaggaat tgttgtcgct gctcgaagcg cttgcggact gtgacgtcgc gaagcacgac
     2101 ggagtagacg acattctcgc cctggtggac ttgcgctatt gcggcctcgg ctggaaactc
     2161 ctcgccgttc ttccggacac ccgcgatctc gcgtcgctca cccatccgtc tcgcggtcac
     2221 ctgggagcgg ccaaattcag caacgtggtg ggggtgaatg gaccgatacc gctccggaat
     2281 cagcgtctcg atacgctgcc cgacgatctc cacggacgtc cagccgaaaa tggcctccgc
     2341 ccctttgttg aagaaggtga tgcgctgaaa tgcatccatg cagatgatcg catccgccgc
     2401 gatctccacg atctctgcca gaatgtcgtg cgttatccgg tcggccatct gtgtccggtt
     2461 atcccctaca aaagggcgtc gtttccctct gaataatcct ccaacccacg acagagtcaa
     2521 cgctcgctga atcgtaagtt gggcatgtgc cggattcacc tcattcgaga ggaagctcca
     2581 tgtccacgaa cgaccaggta aagatgcccg tcgcactgtt cgctaagaag ggttcgcgcc
     2641 gggatttcct gcgaaacacc gccgttaccg caatcgcggg cagtgccctg gccgcgtgtt
     2701 caatagacaa aaccgaagca cagtcgccga gcgcgaacga
//
LOCUS       DATLVW010000138        14596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_49990, whole genome shotgun sequence.
ACCESSION   DATLVW010000138 DATLVW010000000
VERSION     DATLVW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49990"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VNN25_04520"
     CDS             complement(<1..1073)
                     /locus_tag="VNN25_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXH90824.1"
                     /translation="MAPLSRFLFVLLFLTTLGCRGDVAHLDDVGKMVYEVKPAVVRIS
                     AFATAQFRYAPSTIKSIEAALKQDGFGVTAQNLGQNEMDVPTGAGGSGSGFIINPDGL
                     ILTSGHVVAPTRDAATLDRDLLHNGAIAALIKHFPVDELRKLHRTGALDRYIDTLTAN
                     GHLVAREVTNEVDLSNGEILPFTIERFSPPLAQHGTDLALLRVKRKNLPVATLGDSDS
                     TRVSESIWAFGYPAVASSTDDVIGGWLSRDSDLETTLSPGIITAIKRNVANTPVFQSN
                     VAIYQGNSGGPAVNRNGEVVGISTWGRSDAEAIKFLVPINVARSFLAEAKVPINMQGE
                     FDKHYRASLDAAQDGKWTVASSEL"
     gene            complement(1081..2307)
                     /locus_tag="VNN25_04525"
     CDS             complement(1081..2307)
                     /locus_tag="VNN25_04525"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXH90825.1"
                     /translation="MSELVSAEQPVVPTPRLGVQAVLRTDVGLVRSENQDFGTYTTPR
                     EESHSHPGGRLLVVADGMGGHRGGATASRLAGETVKAQYLGSETYDISQALREALARA
                     NARIFSEAQANPDLRGMGTTTSALVVKNNQAWFAHVGDSRIYVVRGDDIRQITEDHSL
                     VASMVREGLLTAKEAETHPRRNVLQRSMGVSEDVDIDVNGPFDVQKDDVFILCSDGLH
                     GLVKEPELKEIAALPIDQAATEYVKRALSRGAPDNVTVIVAKIVAADDPGDATLVDAD
                     ETLADHPYDETQRDTAPIMPLTEPIPAKAEESAPTAAHSSVSAEPATAPGNVPLEHAD
                     AATPGDTLETPVEPDAATAKTIETPIGVNPAPAPRGSLLGWILIIVLALGAAGAWVYW
                     NQHQGAASKTTAPSRR"
     gene            complement(2380..2688)
                     /locus_tag="VNN25_04530"
     CDS             complement(2380..2688)
                     /locus_tag="VNN25_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXH90826.1"
                     /translation="MRVHARDALAIANQIGRTAFLDNRPMQHAVIRCLTVVGEAANRV
                     SEETCRALPDVAWSEAVGLRNVLVHDYHRIDIARIWTIVEEYLPPLLAALDSYLSPIE
                     "
     gene            complement(2708..3022)
                     /locus_tag="VNN25_04535"
     CDS             complement(2708..3022)
                     /locus_tag="VNN25_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH90827.1"
                     /translation="MRHIAIEVDNAKLRDFCRRWKLTELSLFGSVVTGNFGPNSDVDV
                     MVQFADGAPWSLTSWLEMQRELESLFQRHVDLVEREAIERSDNRFRKREILSSAVALD
                     VA"
     gene            complement(3270..4955)
                     /locus_tag="VNN25_04540"
     CDS             complement(3270..4955)
                     /locus_tag="VNN25_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH90828.1"
                     /translation="MPDLAPHQCAAVTRVLSLIGRYGGAILADEVGLGKSFVAATVAA
                     HFQRGGSDIEMIMPASLTGQWRETLRDFAVEARIITHDALAGDPIVGQGDRLIVVDEA
                     HAFRNRHTQRWCALARRSVCARLLLVTATPICNSVDDLLALVSLIAADDALRLRGVAS
                     IDDAFGSRDAQALDVIVRELVIRRERDVLPPELRFGALDRRVIRHPVVDAPIDALQFP
                     LSASAPLLRQFLNRRLESSPAALLESVRRQRHFYERVIESGRPLSRRDYRQAFAADED
                     ADAFQQVLFWDLHAPASGIDLDAIRSELRTLEEIRMIVEQAGDAKVSLLEASLTDDPT
                     LIFTGSAATARALARRLHCGLATARDGRGAIEAFRRGSLDQLVATDLASEGLNLQRAG
                     VVVHYDIPWNPVKLDQRNGRAHRIGQTRDSVRAIYFLPEARSRVVETVASKNRTRRRI
                     LDSRNDVIVTLSPGLLTLPQHLPKDSPALALTRALRSRSLCPPPALMRRYRAGVELLF
                     EAMACEYLDARRLAELEVVLERERALTSSVMTALECSSVQVSGSVESNESLQR"
     gene            complement(4948..8181)
                     /locus_tag="VNN25_04545"
     CDS             complement(4948..8181)
                     /locus_tag="VNN25_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HXH90829.1"
                     /translation="MITAARIQECCSREAVLELFRELGYEVAPVAIALDEWRRAGIEI
                     GWSDAITLDLAARTPELDLYIVSGDPLPEAGAIGAFLRSLTSYNALVKPVVVAVGGVA
                     CRGSGVGAGSGLLGSCVVASKETDPSPTPDSRPATSAPRLEIHDLSSRREHRRLDIDL
                     ARPSAHALDRLNLLASGREPARIFDRALDRESLTRQFFERFRRAVREVSLAIVATRPH
                     ETKDAADGQALLLLSRLLFLYFIQQKGWLNGERRFLIDRIDAAIRNRRGVYTTLFTPL
                     FFGCLNTAVRERDDDAHGLGRIPYLNGGLFEPSPFEERNPSLDLPDELLRRVVEEVFE
                     SFAFSIDESDAAGTHIDPEMLGKVFESLMANEERLSTGSFYTPRAIVDVLAARAIVAW
                     LSDGDDAAMDFLEATLRGESARRPRNARALAQRLQEVAILDPACGSGAFLLGALRVVE
                     RLTLVLEQDVPRDLRRRIVERSLYGVDLKPEAVRLCELRLWLAIVAQTDASADSIQPL
                     PNLDRNILQGNSLLSPTDFLGDNRGDVYREWIYALRAQSDLIARYRNAPHRERASVAR
                     LIRANDGRLAAELVAKAIDLDEREQEQLCAPQRDLFGRLRALNIERCRELYERIGANR
                     RLLDRIEDGEIGFFSFDVHFAHVMARGGFDVVVGNPPWVRNSRIEPGMKRMYAERFPL
                     FHGDGTRGAAAFHQPDLSIAFVERALSLAVQGGVVSLLVPAKLLNAGYAAPLRRASAA
                     NVVALDDWSSDAKRHFDADTFPLGMTLSRRNGATPSPCISVTAAGESFAVSRAALTLQ
                     GSEWSLLPPDVHAIVARIQASHASLGDTLGRTPVMGVKTGDNGSFFLDVGRVRANTIE
                     TIDGLQVPLSAVCRCVRGRDVRRWTIGEPTWMLWPPAGGWPNPPRWVERFAEARGVEV
                     DSLKLSYIRPEHVGIKVVWKDLSRGLCAAVLEDSVRLGERNIPLVPNQTLYAIDAVTI
                     DEAHVIAAMLNSTIVNVLAIAIAERAKDSYFRYFGRTVARLPLPAFFNASSSWNRLLR
                     AARRAAESPAAMEDIDRTVAVLYGVSNEEHERLASFLRKRLGQAGDA"
     gene            8217..9929
                     /locus_tag="VNN25_04550"
     CDS             8217..9929
                     /locus_tag="VNN25_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH90830.1"
                     /translation="MPIRWAYLKRVLAGAIFGLYIANLLYFLNPQIDITPGRLATVTI
                     VYGLICGLLAGTVLWLFRVLRVRLLGKPETDRDYRPHGFGFVVLAAFVATAIYWMHLE
                     VFRIYLPVGAVRVLSKATNVITATSFALLLLWFAERNASRRTSRILFICGVLLIAVSS
                     VFLYQRRDSYRTEAQSVVANVGTVAGQRPVIVVAIRNLPYDWILTMTGEGSLPFFEQA
                     RTHDYVTRLEPFPTISPKSLWASVATGKLPFRHGVTGRLGYRTPLNRPGERFLIIPGG
                     VGFKVWGLIPPVERVATPLPSGLSLPFWSVFERLGIRSAVIGWPWSQPMGASRLVTDR
                     YFESPPRKESEIIPPQFAASVAALKAVPADADTQRFNGTGNARQRIVNSLADDLSSVA
                     ILRASAPSFALTVCTLGGLEQAQRAIHIFSNELPPRSTVKGMVLRAYAEQIDRILAAL
                     ARDYPNHTLVIVSPSGPVPPELPATPYALLRDFVDPADPSADDGFVLIRGPGIVRSDK
                     PRSATAFDVVPTVLYIAGLPVARDMDGSILSDAFSEESLRTNPLSVVQTYEAKRLVVR
                     RGAP"
     gene            complement(10148..10738)
                     /locus_tag="VNN25_04555"
     CDS             complement(10148..10738)
                     /locus_tag="VNN25_04555"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXH90831.1"
                     /translation="MPKRQRGFNLIEILVVVAIIGIIALCAVPSFASFRRRVSCAAAG
                     NEMRSILRAVRSRAITRGANAGVKFVNTGSNWTYTLYDDGNGNGIANADIKSGVDRRY
                     FGPAVTMPLYHIAAIAQLPNGLRDPDGDPLKPDDPALQFGRSTICSFSPLGNGTPGTI
                     YISDGIDQLYAVRVFGPSGRVRLLRYNSGNRKWEER"
     gene            complement(10880..11287)
                     /locus_tag="VNN25_04560"
     CDS             complement(10880..11287)
                     /locus_tag="VNN25_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXH90832.1"
                     /translation="MRKSHLIVLSAILILSMSTAVMAADTQQTQAAPTGVVNINSADV
                     AQLAFLPRVGVKAAQRIIDYRKEHGSFKKTSDLMQVKGFGEKSYERLAGYLSVDGKTT
                     LAAKVHAGRLGGGKRTKSVKPSKSSSSPSPATR"
     gene            complement(11580..13403)
                     /gene="asnB"
                     /locus_tag="VNN25_04565"
     CDS             complement(11580..13403)
                     /gene="asnB"
                     /locus_tag="VNN25_04565"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH90833.1"
                     /translation="MCGINGIVSFRAGAPLVDLDELTRTREAMRARGPDAAGSWMSSD
                     GRTGFGHRRLSIIDVSARANQPMVSRDGRMVLTFNGEIYNYAELRADLEAEGETFDTT
                     SDTEVVLRMYAKWGDAMLPRMRGMFAFAIWDARTRTLFVARDPYGIKPLYYANDGATF
                     RFASQVKALLAGGNVSATRDPAGIVGFLLRGNIPEPFTIYEAIRELPAGSSMIVTESG
                     PSEPRSYFSIAGILREAVKENVRFSEEERAAIIADAVRESVRYHLVADVPVGAFLSAG
                     RDSGTITALAMESIRDLHTVTLRFAEYDGTPKDEAPLAAVVAREYGTIHRTSTLSSEA
                     FHTALPQALEAMDQPTLDGLNSYFVCKAAAELGWKVALSGTGGDELFGGYSTFRIIPR
                     VVRMFGAFRYMPRLAGAFARMQPKMARTEARFSPKTAYTLKYCTSYEGAYLMKRGLFL
                     PDDVASVVGDEMACEGLKRLRILDLIRETITPDPGTPVARVMALESSLFLRSQLLRDI
                     DWASMAHSLEVRVPLVDAFLLRQVAPAIVANGRLNGKDLLAASPRQPLPAAILQRKKS
                     GFTVPIRTWLGHDREAKSQFGGRPWASYVLEAGGHLPATLA"
     gene            13655..>14596
                     /gene="clpX"
                     /locus_tag="VNN25_04570"
     CDS             13655..>14596
                     /gene="clpX"
                     /locus_tag="VNN25_04570"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXH90834.1"
                     /translation="MKEKRDIYRFLHVTPRRIYEQLDQYVVGQKHAKEVLAIAAYNHL
                     KRIEYLKFGGDVSIRKSNILMIGPSGSGKTYLARTLAKILDVPFAYNDATAFTEAGYY
                     GEDVEVAIGRLLFATNQNVDAAENGIVFIDEIDKIARRSGGTRTGSGSRDIGGEGVQQ
                     SLLKILEGEKLFVPLNVTSHWNKYDYVEIDIANILFICAGAFSDIDDNSDAKPIGFFG
                     DGDVPAKDISTEELVKYGFLPELLGRLPVHVELDSLTADDLVTILTEPREAMIPEYQR
                     LCALDQIQLEFSAGALYAIAEAALKQKLGARALRAILE"
BASE COUNT         2678 a         4613 c         4704 g         2601 t
ORIGIN      
        1 agctcgctcg atgccacggt ccacttgcca tcctgcgcgg cgtcaagcga tgcgcggtaa
       61 tgcttgtcga actccccctg catgttgatc ggcacctttg cctcggcaag aaaactccgg
      121 gcgacgttga tcggaacgag aaacttgatc gcctccgcgt cgctgcggcc ccaggtcgag
      181 atgccgacga cttcgccgtt ccggttgacg gctggcccgc ccgagttgcc ctgatagatg
      241 gcgacgttcg actgaaacac cggcgtgttg gcgacgtttc gtttgatggc ggtgatgatg
      301 ccgggactga gagtcgtctc gaggtcgctg tcgcgcgaaa gccagccgcc gatgacatcg
      361 tcggtgctgc tggccacagc gggatagccg aacgcccaga tcgactcgct cacacgagtg
      421 gagtcggagt cgccgagcgt cgccacgggc aagtttttcc gtttcacgcg cagaagggcg
      481 agatcggtcc catgctgcgc caacggcggc gagaagcgct cgatcgtgaa cggcagtatc
      541 tcgccgttcg acagatcgac ttcgttggtc acttcacgcg ccacgagatg gccgttggcg
      601 gtcagcgtgt cgatgtagcg atcgagcgca ccggttcgat gaagtttgcg aagctcgtcg
      661 accggaaagt gtttgatcag cgccgcgatc gctccgttgt gaaggagatc gcgatcgagc
      721 gtggcggcgt cgcgcgtcgg tgcgacgaca tgcccgctcg tgagaatgag gccatcggga
      781 ttgatgatga agccgctgcc gctgccgcca gctcccgtcg gcacatccat ttcgttctgg
      841 ccgaggttct gcgcagtgac gccgaagcca tcctgcttca gcgccgcttc gatgctcttg
      901 atcgtcgacg gcgcgtagcg gaattgcgcg gtcgcgaacg cgctgatgcg cacgaccgcc
      961 ggcttcacct cgtagaccat cttcccgacg tcatcgaggt gcgccacatc gccgcggcaa
     1021 ccgagcgttg tgagaaacag aaggacgaag aggaagcggg acaggggtgc catcgggagg
     1081 ttaccggcgt gagggagcgg tggttttgga ggcggctcct tgatgttgat tccagtagac
     1141 ccaggcgcca gcggcaccga gcgcgagaac gatgatgaga atccagccga gtagcgatcc
     1201 ccgaggggcc ggggcgggat tgacgccgat cggcgtttcg atcgtctttg ccgttgctgc
     1261 gtccggctcg acgggcgtct cgagcgtatc gccgggagta gcggcatcgg cgtgttctag
     1321 cggcacattg cccggcgcag tggctggctc tgcggagacc gaggaatgcg ccgccgttgg
     1381 cgcggactcc tcggcctttg caggaatcgg ttcggtcagc ggcatgatgg gtgccgtgtc
     1441 gcgctgtgtc tcatcgtagg ggtgatcggc cagcgtctca tcggcatcga cgagcgtcgc
     1501 gtcgccgggg tcatcggcag cgacgatctt cgccacgatc acggtgacgt tgtccggcgc
     1561 tccccgcgac agcgcgcgct tcacgtactc ggtggccgcc tgatcgatcg gcagggcagc
     1621 aatctctttc agctccggct ctttgacgag gccgtgcagg ccgtcgctgc agagaatgaa
     1681 gacgtcgtcc ttctgcacgt cgaacgggcc attgacgtcg atatcgacgt cctcgctaac
     1741 tcccatcgaa cgttgcagga cgttgcggcg cggatgcgtc tcagcctctt ttgcggtgag
     1801 cagcccttcc cgcaccatcg acgcgacaag cgagtgatcc tcggtgatct ggcggatgtc
     1861 atcgccgcgg actacgtaga ttcgcgagtc gccgacgtgt gcgaaccagg cttggttgtt
     1921 cttgacgacg agcgccgaag tcgtcgtccc catccctcgg agatccggat ttgcttgcgc
     1981 ctcggagaag atccgtgcgt tggcccgagc cagcgcctcg cgcaacgcct gggagatgtc
     2041 gtaggtctcg ctgccgagat attgagcctt caccgtctca ccggcgagcc gcgatgcagt
     2101 ggcgccgccg cgatgcccgc ccatgccgtc cgcgacgacg agcagccgtc cgccaggatg
     2161 actgtggctc tcctcacgcg gcgtcgtgta ggtgccgaag tcctgattct ccgagcgcac
     2221 cagccccaca tcggtgcgca ggactgcctg aacaccgagg cgcggcgtgg ggacgaccgg
     2281 ctgttctgcc gagacgagct cgctcatatg cgtggagaca ggcgaggatt atacagggtt
     2341 gagggggaag ggaatcgggg gcgggaaccg atccgtgact cactcaatcg gggagagata
     2401 ggaatcgagg gcggcgagga gtggcggaag gtactcttca acgattgtcc agattcgggc
     2461 gatatcgatg cggtggtagt cgtggacaag cacgttgcgt agtccgacgg cttcgctcca
     2521 ggccacgtcc ggcaatgccc ggcatgtctc ttccgagact cgattggcag cctcgccgac
     2581 gacagtcaag cagcggatga ctgcatgttg catcggccgg ttatcgagaa aggcggtacg
     2641 cccgatttgg tttgcgatgg caagggcgtc tcttgcatgt acgcgcatgt cctcgagata
     2701 cccgaggtca cgcaacgtca agggccaccg ccgatgagag gatctcgcgc ttccggaagc
     2761 ggttgtcgct tcgttcgatc gcctcgcgct cgacgagatc gacgtggcgc tgaaagagtg
     2821 actcaagctc ccgctgcatc tccaaccagc tggtcaggct ccatggcgcg ccatctgcga
     2881 actgcaccat cacgtcaaca tcgctgtttg ggccgaagtt gcccgtcacg acgctgccga
     2941 aaagagagag ctctgtgagc ttccagcgac gacagaagtc gcgtagtttg gcgttgtcga
     3001 cctcgatcgc gatgtggcgc atggaaggaa agtttaccgg cttttcccaa gcgccgcgag
     3061 gcgtgcaggt cgtaggagtg cggtcgtctc gaccgcgggc actcgggcgt cctcgcccga
     3121 gtgctgtcgg ggcgaagtcg aaattcgcag acgttgctgg attgtgagag gtcaaatgaa
     3181 aagacgtgat tttgatgagc tcgtagaaag cgtacgggaa gccgggcaaa tccttcgcgg
     3241 agaggtcggg cctgctcgca agttcctgtt taccgctgaa gagactcgtt cgattcgaca
     3301 gagcccgaga cctgcacgga agagcactcc agcgcagtca tcacggagga agtgagcgct
     3361 cgctctcgct ccagtaccac ctccaattcc gcgagccggc gtgcgtcaag gtattcgcag
     3421 gccatggctt cgaagagcag ctcgaccccg gcgcgatagc ggcgcatgag ggcgggaggc
     3481 gggcacaggc ttcgtgaacg gagggcgcgt gtcaacgcca gcgccggcga atctttcggg
     3541 aggtgttggg ggagggtcag caagcctggc gagagcgtca cgatgacatc gtttcgtgaa
     3601 tcgagaatcc gcctccgtgt ccggttcttc gacgcgaccg tttcgacgac gcggctccgc
     3661 gcttcgggca gaaagtagat ggcgcgaacg gaatcgcggg tctggccgat gcggtgcgcg
     3721 cggccgttgc gctgatcgag tttcaccggg ttccagggga tgtcgtaatg aacgacgacg
     3781 cctgcccgct gcaggttcaa cccttcggaa gcgagatcgg tcgcgacaag ctggtcgagc
     3841 gatccgcggc gaaaggcctc gatcgcgccg cgtccgtcgc gcgcggtggc cagtccacaa
     3901 tgaagccggc gcgccaacgc gcgtgctgtc gccgcggagc cggtgaagat aagggtggga
     3961 tcatcggtca gcgacgcttc gagcagcgac actttcgcgt ccccggcttg ctcgacgatc
     4021 attcgaatct cctcgagggt gcgcagttcg cttcgaatcg cgtcgaggtc gatccctgat
     4081 gccggcgcgt gtaagtccca gaaaagaacc tgctggaacg catccgcatc ttcgtcggct
     4141 gcgaacgctt gccggtaatc gcgccgcgaa agagggcgcc cgctttcgat gacgcgctcg
     4201 tagaaatggc gctggcggcg aacggattcg agcagcgccg cggggctcga ctcgaggcgc
     4261 ctattgagaa actgccgcag cagtggtgcg ctggcgctca gcggaaactg caatgcgtcg
     4321 attggcgcat cgacaacggg atggcggatg acgcggcggt cgagcgcgcc gaagcgcagc
     4381 tcgggcggca gtacgtcgcg ctcgcgccgg atgacgagct cgcgcacgat cacgtcgagc
     4441 gcctgcgcgt cgcggctgcc gaatgcgtca tcgatcgaag cgacgccgcg aagccgcaaa
     4501 gcatcatccg cagcaatgag cgagacgagc gcgagaaggt cgtcgacaga gttgcagatc
     4561 ggcgtcgccg tgacgagcag cagcctcgcg caaacggagc ggcgcgccaa ggcacaccac
     4621 cgctgcgtgt ggcgattgcg gaatgcgtgc gcctcgtcga cgacaatgag ccggtcgccc
     4681 tgaccgacga tcggatctcc cgcgagggcg tcgtgcgtga tgatccgcgc ctcgactgcg
     4741 aagtcgcgca acgtctcgcg ccattgcccg gtcagcgacg caggcatgat catctcgatg
     4801 tctgatccgc cgcgctgaaa gtgtgcggcc accgtcgctg ccacaaacga cttgccgagg
     4861 ccgacttcgt ccgcgagaat cgccccgccg tagcggccga tcagcgacag cacgcgcgta
     4921 acggccgcgc actggtgcgg cgccaggtca ggcatcgccg gcctggccaa gccgcttgcg
     4981 aaggaacgac gccagccgct cgtgctcttc gttggaaaca ccgtagagca cagccacggt
     5041 tcgatcgatg tcctccatcg cggccggact ctccgctgcg cgccgggccg cgcggagcaa
     5101 ccggttccag gaggagctcg cgttgaagaa ggccggaagc ggcaagcgcg cgacagtgcg
     5161 tccgaaatag cgaaagtacg agtctttggc gcgctcggcg atggcgatgg ccaggacatt
     5221 gacgatcgtc gagttcaaca tcgctgcaat gacatgcgcc tcgtcgatcg tcaccgcatc
     5281 gatcgcatac aacgtctggt tcggaacgag cgggatattg cgctcgccga gccgcacgga
     5341 atcctcgagc actgcagcgc aaagaccgcg cgaaagatct ttccacacca ccttgatgcc
     5401 gacatgctcg ggacgaatgt aggacaattt cagcgaatcg acttcgacgc cgcgcgcctc
     5461 ggcaaagcgc tcgacccaac gcggtggatt cggccaacct cccgccggcg gccagagcat
     5521 ccacgtcggt tcgccgatcg tccagcgccg cacatcgcgg ccgcggacgc agcggcagac
     5581 ggcgctgagc ggaacctgaa ggccgtcgat ggtctcgatg gtgttcgcgc ggacgcgccc
     5641 gacatcgagg aaaaacgagc cgttgtcgcc ggtcttcaca cccatcaccg gcgtgcggcc
     5701 gagagtgtca ccgagtgacg cgtgcgacgc ttgaatgcgc gcaacgatcg cgtgcacatc
     5761 gggcggcaga agggaccatt cggagccttg caatgtcagc gcggcgcgcg aaacagcgaa
     5821 ggattcgccg gccgcggtga cggaaatgca tggcgacgga gtggcaccgt tgcgccgcga
     5881 cagcgtcatg cctagcggga acgtgtcggc atcgaagtgg cgcttggcat cggaggacca
     5941 atcgtcgagc gcgacgacgt tcgccgccga cgcgcgccgc aacggcgccg catagccggc
     6001 attgagcaac ttggcgggca ccaggagcga gacgacgcct ccctgcaccg ccaacgagag
     6061 cgcgcgctcg acgaatgcga tcgaaagatc gggctgatga aatgctgccg cgccgcgcgt
     6121 gccgtcgccg tgaaagagag gaaaccgctc cgcgtacatt cgtttcatgc ccggctcgat
     6181 gcggctgttg cgaacccagg ggggattgcc gacgacgacg tcgaatccgc cgcgcgccat
     6241 cacatgggcg aagtggacgt cgaaggagaa gaatccgatc tcgccatcct caatccgatc
     6301 gagcagacgg cggttcgcgc cgatgcgctc gtacagctcg cggcaccgct cgatgttcag
     6361 cgcgcgaagg cggccgaaga gatcgcgttg cggcgcgcag agctgttcct gttcgcgctc
     6421 atcgaggtcg atcgccttcg ccacgagctc cgcggcgagg cgtccgtcgt tcgcacggat
     6481 gagtcgcgcg acggaggcac gctcgcgatg cggcgcgttg cggtagcggg cgatgaggtc
     6541 cgactgcgcc cgaagcgcat agatccattc gcgatagacg tcgccccgat tgtcgccgag
     6601 aaaatcggtt gggctcagga gcgagttgcc ctgcaggatg ttgcggtcga ggttcggcaa
     6661 cggctggatg ctgtcggcgg atgcatccgt ctgcgcgacg atggccagcc acaatcgcaa
     6721 ctcgcagaga cgcaccgcct ccggtttcag atcgacgccg taaagcgaac gctcgacgat
     6781 gcgccggcgg agatcgcgag ggacatcctg ctcgagcact agcgtgagcc gctcgacgac
     6841 acgaagcgcg ccgagaagaa acgcgccgga accacaggcc ggatcgagga tggcaacctc
     6901 ttgcagacgc tgcgcgagcg cacgggcatt gcgcgggcga cgcgcagact cgccgcgaag
     6961 cgtagcctcc aggaagtcca tcgccgcgtc gtcgccatcg ctcagccacg ccacgatcgc
     7021 gcgggccgcc agaacgtcga cgatggcgcg cggcgtgtag aagctgccag tggacagccg
     7081 ctcctcgttc gccatcaacg actcgaatac ttttccgagc atctccggat cgatgtgtgt
     7141 tccggccgca tccgactcgt cgatcgaaaa cgcgaaactc tcgaacacct cctcgaccac
     7201 gcgccgcagc agttcgtccg gcagatcgag actcgggttt cgttcttcga agggagaggg
     7261 ctcgaagaga ccgccgttga ggtacgggat ccggccgagc ccatgcgcgt cgtcgtcacg
     7321 ctcgcgaacg gccgtgttga ggcagccgaa aaagagcggc gtgaaaagtg tggtgtaaac
     7381 gccgcgccgg ttgcggatgg cggcatcgat gcggtcgatc agaaagcgcc gttcgccatt
     7441 gagccaccct ttctgctgga tgaagtagag aaacaggagg cgcgagagga gcagcagcgc
     7501 ctgaccatcg gcggcgtctt tcgtctcatg cggacgtgtc gcgacgatcg ccagcgagac
     7561 ctcgcgcacc gcgcggcgaa agcgctcgaa gaactggcgc gtcaacgact cgcggtcgag
     7621 ggcgcggtcg aagatgcgcg ccggctcgcg gccggaagcg agcaggttga gcctgtcgag
     7681 tgcgtgtgcc gacgggcgtg ccaggtcgat gtcgagtcgc cgatgctcgc gccgcgatga
     7741 aagatcgtga atctccagcc gtggtgcgga agtcgctggt cgggagtcgg gcgtcggaga
     7801 tgggtctgtt tccttgctcg caacaacgca ggaaccgagc aacccggaac ccgctccgac
     7861 tcccgacccc cgacaagcga cgccccctac tgcgacgacc accggcttga ccagcgcgtt
     7921 gtacgacgtg agcgaacgga ggaacgcccc gatcgcaccc gcctccggca gtggatcgcc
     7981 gctgacgatg tagagatcga gctccggcgt acgcgcggca agatcgagcg tgatggcgtc
     8041 gctccatccg atctcgatcc cggcccggcg ccactcgtcc aatgcgatgg cgactggcgc
     8101 gacctcgtac ccgagctcgc ggaaaagttc gagcactgcc tcccgcgagc agcattcttg
     8161 gatgcgtgcc gcggtgatca cgccgcacct ccgcgcgata cactgcgcgg gccggcatgc
     8221 cgattcgttg ggcgtatctc aagcgcgtac tcgcaggtgc gatcttcggc ctctacatcg
     8281 ccaaccttct ttactttctc aacccgcaga tcgacatcac acccggtcgc ctggcaacgg
     8341 tcacgatcgt ctatggcctg atctgcggcc tgctcgccgg tacagtcctc tggctcttcc
     8401 gcgtccttcg cgtacgcctc ctcggcaaac cggaaactga ccgcgactac cggccacacg
     8461 gcttcggttt cgtcgttctg gccgcgttcg tggccacggc catctactgg atgcacctcg
     8521 aggtgttccg catctatctg ccggtcggcg ccgtgcgagt gctgtcgaaa gcgacgaacg
     8581 tgatcaccgc gacctcgttc gcgctcctgc tgctctggtt tgccgagcgc aatgccagcc
     8641 gccgcacctc gcgcatcctc ttcatctgcg gcgtgctgct gatcgcggtt tcgtccgtct
     8701 tcctctacca gcgccgcgac agttaccgca ccgaggcgca gtcggtggtg gcgaatgtcg
     8761 ggacggtggc cggccagcgt cccgtgatcg tcgtcgccat ccggaatctg ccttacgact
     8821 ggatcctcac gatgaccggc gagggctcgc tgccgttctt cgagcaggca cggactcacg
     8881 actacgtcac gcgcctcgag cccttcccca cgatcagccc gaagtcgctg tgggcctcgg
     8941 tcgcgaccgg gaagctgccg ttccgccacg gcgtcacggg acgcctcggc tatcgcacgc
     9001 cgctcaatcg tccaggcgaa cgattcctga tcattccggg cggtgtcggc ttcaaagtgt
     9061 ggggactgat tccgccggtc gagcgcgtgg ccacgccgct tccctccgga ctctctcttc
     9121 ctttctggtc ggtcttcgag cggctcggca ttcgttccgc ggtgatcggc tggccatggt
     9181 cgcaaccgat gggagcgtcg cgccttgtca ccgatcgcta cttcgaaagc ccaccgcgaa
     9241 aggagagcga gatcattcct ccgcagttcg ccgccagcgt ggcggcactc aaggccgtgc
     9301 ctgcggatgc ggatacgcag cgcttcaacg gcacgggcaa cgcgcgccag cggatcgtga
     9361 acagtctggc cgacgacctc agctcggtcg ccatcctccg cgcctcggcg ccgagctttg
     9421 cgctcaccgt ctgcacgctc ggtgggctgg agcaggcaca acgcgccatt cacattttca
     9481 gcaacgagct tccgccccgc tcgaccgtga aggggatggt cctgcgcgcg tacgcggaac
     9541 agatcgatcg gatcctggcg gcgctcgcgc gcgactatcc caatcacacg ctcgtgatcg
     9601 tgtcgccgtc cggacccgtc ccgccggaac tgcccgcgac cccttacgcg ctgctccgcg
     9661 atttcgtcga tccggccgat cccagcgccg acgacggctt cgtgctgatc cgcggtcctg
     9721 gcatcgtccg cagcgacaag cctcgctccg caaccgcctt cgacgtcgtg cccaccgttc
     9781 tctacatcgc cggccttccc gtcgcccgcg acatggacgg cagcatcctc tccgacgcct
     9841 tctccgagga gtcgttgcga acgaatcccc tgtcggtggt gcaaacctac gaagcaaaga
     9901 gactcgtagt gcggcgcgga gcaccgtaac tccaacgcag ctctaccatc gaacgcctta
     9961 cttcgagcaa atcgcggtgc tctatcatcg agcgccttct ccccccgcgc tttttgcggg
    10021 gggagaaggt ggcgaagccg gatgaggggg gcgagacact ccgaaagaaa gatgttcctg
    10081 cgccgtggga agagtgtctg ctcaagggag gaggcagcac gccgtacgca ggaagagcga
    10141 acgactctca tcgctcctcc cacttccgat tcccgctgtt gtagcgcagc agtcgcaccc
    10201 ttccgcttgg gccaaacacg cgaaccgcgt agagctggtc gatgccgtcg ctgatgtaga
    10261 tcgtccccgg agtgccgttt cccaacggcg agaacgagca gatcgtcgag cggccgaatt
    10321 gaagggcagg atcgtcgggc ttgagcggat cgccatcggg atcgcgcaat ccgtttggca
    10381 gctgcgcgat cgcggcgatg tggtacagcg gcatcgtcac cgccgggccg aagtagcgcc
    10441 ggtcgacgcc ggacttgatg tcggcattgg cgattccgtt gccgttgccg tcgtcgtaaa
    10501 gcgtgtaggt ccagttgctc cccgtattca cgaacttcac gccggcattc gctcctcggg
    10561 tgattgcacg cgaacggact gcacgcagga tgctgcgcat ttcgttgccc gccgccgcgc
    10621 aggagacacg ccgcctgaag ctggcgaagg agggaaccgc gcaaagggcg atgattccga
    10681 tgatggcgac gacgaccagg atctcgatca agttgaaacc gcgttgacgt ttaggcacac
    10741 gttctccttt cgggtgatca ggtggttggg gatcagccgg cagacagcag cgatcggtct
    10801 ggccgctgat ctctgacgct gacctctccg aggtggccgc cccgcttgcg cgaggcggcc
    10861 tttttggagg ggcggcgcgt taccgcgtcg cgggggaggg cgatgaggac gacttcgaag
    10921 gcttgacgga cttggtgcgc tttccgccac cgaggcggcc ggcgtgaacc ttcgcggcca
    10981 gcgtcgtctt gccgtcgacc gaaaggtagc cagccagacg ctcgtacgac ttctcgccga
    11041 agcccttgac ctgcatcagg tccgaggtct tcttgaagct gccgtgctcc ttgcgatagt
    11101 cgatgatgcg ctgtgccgct ttgacgccga cgcgcggcag gaatgcgagc tgcgccacgt
    11161 ccgcgctgtt gatattgacg acgcctgtcg gtgcggcctg ggtctgctgt gtgtcggccg
    11221 ccatgaccgc ggtcgacatc gagaggatga gaattgccga aaggacgatg aggtgggact
    11281 tccgcatggt ctttttctcc ttatttgttg gtggttgatt ggttggttga acgttgacgc
    11341 gtttcgttgt tttggctgtt tcccacacga ggaccgttga tcccctacat cagatgaaaa
    11401 ctcgtggctc cgtttggcac cacctccttt cgcggcttct tagtgcgatc ggacggccag
    11461 cgtgcctaaa cgtgcaagtc attgcgaaaa cgcgacttac gcgaaaacgc gaagcggcgc
    11521 agcgtcaaga atgacgctgg agagcaaagg atttttacgt gaggcttgtc tgacggatgt
    11581 caggccagcg tggcaggaag atggccgccc gcttcgagca catagctcgc ccacggccgg
    11641 ccgccgaatt gcgatttcgc ttcgcggtcg tggccgagcc aggtgcggat ggggaccgtg
    11701 aagccgctct tcttgcgctg gagaatcgct gccggcagcg gctgccgcgg cgaggcggca
    11761 aggaggtctt ttccgttgag gcggccgttt gcgacgattg cgggagcaac ttgccggaga
    11821 agaaaagcgt cgactagcgg tacgcgtact tcgagcgaat gcgccatcga ggcccagtcg
    11881 atgtcgcgaa gcagctggct tcgcaggaac aacgacgatt cgagcgccat gacgcgtgcg
    11941 accggcgttc cgggatcggg cgtgatggtt tcgcggatga ggtcgagaat gcggagacgc
    12001 ttcaacccct cgcatgccat ctcgtcgccc accacggacg caacgtcatc cggcaggaag
    12061 aggccgcgct tcatcagata ggcaccttcg tacgacgtgc agtacttcaa tgtgtacgcc
    12121 gtcttcgggc tgaaacgggc ctcggttctg gccatcttcg gttgcatccg ggcgaaggca
    12181 ccagccaggc gtggcatgta ccggaacgcg ccgaacatgc gcaccactct cgggatgatg
    12241 cggaaggtcg agtagccgcc gaacaactcg tcaccacccg tgcccgagag cgcgaccttc
    12301 cagccgagtt ccgcggctgc cttgcaaacg aagtaggagt tgagcccgtc gagagtcggc
    12361 tgatccatcg cctcgagcgc ctgcggcagc gcagtgtgaa acgcctcgga cgagagtgtc
    12421 gacgtccggt gaatcgttcc gtactccctg gccaccaccg cggccagcgg cgcttcgtcc
    12481 ttgggagttc cgtcgtattc cgcaaagcgg agagtcaccg tgtgcaggtc gcggatgctc
    12541 tccattgcca gggcggtgat ggtgccggag tcgcggccgg cggagagaaa cgcgccgacg
    12601 ggaacgtcgg cgacgaggtg gtagcgaacc gactcgcgga cagcgtcagc gatgatcgca
    12661 gcccgttcct cctcgctgaa acgaacgttc tccttcacag cttcgcgaag gattccggca
    12721 atcgaaaaat aggagcgagg ctccgacgga ccactctccg tgacgatcat cgagctgcct
    12781 gccggcaatt cgcggatcgc ttcgtagatc gtgaagggct caggaatgtt gccgcgcagc
    12841 agaaatccga cgatgccggc cggatcgcgc gtcgccgata cgtttccacc agccagaagg
    12901 gctttgacct gcgacgcgaa acggaacgtc gccccgtcgt tcgcgtagta gagcggcttg
    12961 atcccgtacg gatcgcgggc aacgaacagc gtccgcgttc gcgcatccca gatcgcgaag
    13021 gcgaacatgc cacgcatccg agggagcatg gcgtcgcccc actttgcgta cattcgcaga
    13081 accacttccg tgtctgacgt cgtatcgaac gtctcgccct cggcctcgag gtccgcgcgc
    13141 aactccgcgt agttgtaaat ctctccgttg aacgtcaaca ccatccggcc atcgcggctg
    13201 accatcggct gattggcgcg ggcgctgacg tcgatgatgg agaggcgccg gtgaccgaaa
    13261 ccggtacgtc cgtcgctcga catccacgaa ccggctgcgt ccggaccacg cgctcgcatc
    13321 gcctcccgtg ttcgcgtcaa ctcatcgagg tcgaccaacg gtgcgccggc gcgaaaggag
    13381 acgatgccgt tgattccgca catgaagggg cggcagtctc acacatctgg cttaaatccc
    13441 tcatgaagcg ggaagcagag atcagaggac cagagacaag agatcaagga accagagatc
    13501 agggaaccag agacaagaga tcagggggaa gcacggttcc tctccgatgt ctacatctag
    13561 tctgcatctt cctaatcgct gacctctgat ctctgatctc tagtctccgc tcctctgatc
    13621 tctcctcccc gggcttatac tcgactcgcc gcccatgaag gaaaagcgtg acatctaccg
    13681 cttcctgcac gtcacaccgc ggcgcatcta cgaacagctc gatcaatacg tcgtcggcca
    13741 gaagcacgcc aaagaagtcc tggccatcgc cgcgtacaac catctcaagc gaatcgagta
    13801 cctgaagttc ggcggcgacg tctccatccg caagtcgaac atcctgatga tcggcccctc
    13861 gggatcgggc aagacctacc tggcccgcac cctggccaag atccttgacg ttcccttcgc
    13921 ctacaacgat gcaactgcgt tcaccgaggc tgggtattac ggggaggatg tcgaggtcgc
    13981 gatcggaagg ctgctctttg ccacgaatca gaacgttgac gccgcggaga acggaatcgt
    14041 cttcatcgac gagatcgaca agatcgcgcg gcgctccggc ggcacgcgca ccggctccgg
    14101 atcgcgcgac atcggcggcg aaggcgtgca gcagtcgctg ctcaagatcc tcgagggcga
    14161 gaagctcttc gttccactga acgtcacgtc acactggaac aagtacgact acgtcgaaat
    14221 cgacatcgcc aacattctct tcatctgtgc cggcgcgttc agcgacatcg acgacaacag
    14281 cgacgccaag ccgatcggct tcttcggcga tggcgatgtt cccgcgaaag acatctccac
    14341 cgaagagttg gtcaagtacg gcttcctacc cgagctcctc ggacgccttc ccgtccacgt
    14401 cgagctcgac tccctcaccg ctgacgatct cgtcaccatc ctcaccgagc cacgcgaggc
    14461 gatgatcccg gagtaccagc gcctctgcgc gctcgaccag atccagctcg agttctccgc
    14521 cggcgcgctc tatgcgatcg ccgaggcggc gctgaaacag aagctcggcg cgcgggcgtt
    14581 gcgcgccatc ctggag
//
LOCUS       DATLVW010000139         2795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_5114, whole genome shotgun sequence.
ACCESSION   DATLVW010000139 DATLVW010000000
VERSION     DATLVW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5114"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VNN25_04575"
     CDS             <1..281
                     /locus_tag="VNN25_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90835.1"
                     /translation="FCTPGDLFCTPGDLFCTPGDLFCTPSDLFCTPGDLFCTPEEVRC
                     TPDEAHCTPDEVHYTPEEVHCAPDEVHCTHDAVFPISDSVFHTVDAAS"
     gene            complement(417..959)
                     /locus_tag="VNN25_04580"
     CDS             complement(417..959)
                     /locus_tag="VNN25_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90836.1"
                     /translation="MPADARAKLVRQLQIAYSAELGAGYAYRGHWHSVRDPGERDHIR
                     QIEGEEWHHRELVGGLLQQLGGKPSPLREFIFWWIGHFIAGFCHVGGWFAPMYGAGRL
                     ERGNIVEYEDAAVFAAECGHDEMIDCLLTMAEVEWDHEAYFRTKAASHPFVRILKMWP
                     ATEPKEAIRARFAENEKATA"
     gene            1005..1943
                     /gene="fabD"
                     /locus_tag="VNN25_04585"
     CDS             1005..1943
                     /gene="fabD"
                     /locus_tag="VNN25_04585"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXH90837.1"
                     /translation="MEHNTAFVFPGQGSQYAGMGKDVAAAFPAARRVFDDIDAALGFS
                     ISQLCFEGPDDQLKLTENTQPAILAVSSAIHAVLEERGATRRDVVAGHSLGEYSAIVS
                     VGGLTPADAAQLVRMRGRFMQEATPVGSGGMAALIGPSVEEVTSICEEAAQGEVVSIA
                     NINAPGQIVIAGTKAGIDRAIAVAKARGVRRALPLPVSAPFHCALMKPAEERLKPLLD
                     DAPFKDLWMSLVSNVDASPIGTSTAVRNALVRQVVSPVRWVESVQKMVSMGVRRFVEI
                     GPGAVLTGLIKRIDPSVELINVSDVPSIEKFLESMQ"
     gene            1940..2680
                     /gene="fabG"
                     /locus_tag="VNN25_04590"
     CDS             1940..2680
                     /gene="fabG"
                     /locus_tag="VNN25_04590"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXH90838.1"
                     /translation="MKIDLTGRTALVTGASRGIGEAIALRLAESGAHVLCAARNLEHV
                     ERVAGVITANGGSAAAVQLDITSDDIRERIKALIEQQPIDILVNNAGITDDDLFIRMK
                     PEAWTSVLRTNLDSAFHITQEVVKKMIRARWGRVINISSIVGLSGNPGQVNYASSKAA
                     LIGFTKALALEIGSRSVTVNAIAPGFVKTAMTDAMTDDARKTLEARIALHRLGTVDDI
                     AYAAVFLASEQAGYITGTVMKVSGGLYT"
BASE COUNT          493 a          925 c          862 g          515 t
ORIGIN      
        1 tgttctgcac ccccggtgac ttgttctgca cccccggtga cttgttctgc acccccggtg
       61 acttgttctg cacccccagc gacttgttct gcacccccgg cgacttgttc tgcacccccg
      121 aagaagtgcg ctgcaccccc gatgaagcgc actgcacccc cgatgaagtg cactacaccc
      181 ccgaagaagt gcactgcgcc cccgatgaag tgcactgcac ccacgatgct gtgtttccca
      241 tctccgattc tgtctttcac acagtcgatg ctgcgtcttg aaccatcgat gctgcgtctc
      301 gacccatcgt cggtgctttt tgatccgtcg atgctgggtt ttgaatcatc gacgcttgtt
      361 tcgaaccacg acacctatga ggatgacggc aaagggatac accacttccg ccgcacctac
      421 gccgtcgcct tctcgttctc cgcgaaccgg gcccggatcg cttccttcgg ctcggtcgct
      481 ggccacatct tgaggatccg cacgaacgga tggctggccg ctttggtccg aaagtacgct
      541 tcgtgatccc actccacctc ggccatcgtc agaaggcagt cgatcatctc gtcgtggcca
      601 cactccgccg cgaacaccgc cgcatcctcg tactcgacga tgttgcctcg ctccagccgg
      661 ccggcgccgt acatcggtgc gaaccagccg ccgacgtggc agaagccggc gatgaagtgg
      721 ccgatccacc agaagatgaa ttcgcgcaga gggctcggtt ttccgccgag ctgctggagc
      781 aagccgccga cgagctcgcg gtgatgccac tcctcgcctt caatctgccg gatgtgatct
      841 cgctcgccgg ggtcgcggac gctgtgccaa tggcctcgat acgcatagcc ggccccgagc
      901 tccgccgagt aggcgatttg gagttggcgg acgagttttg cgcgggcgtc ggcgggcatt
      961 cgcagcgatt gtagccgctc cggtccatgt atgattccgc gccgatggag cacaacacag
     1021 ctttcgtttt ccccggacag ggaagccagt acgccgggat gggaaaagat gtcgctgcgg
     1081 cgtttcccgc ggcgcgccgc gttttcgacg acatcgacgc cgcactcggc ttttccatct
     1141 cgcaactctg cttcgaagga cccgacgatc agctcaagct gacagagaac acgcagccgg
     1201 cgattctggc tgtgtcgtcc gcaattcacg cggtgctcga agagcgcggc gccacccggc
     1261 gcgatgtcgt ggccggccac tccctcggcg aatacagcgc gatcgtcagc gtcggcggat
     1321 tgacgccggc cgatgcagcg caactcgtcc ggatgcgcgg ccgtttcatg caggaggcca
     1381 cgcccgtcgg cagcggcggc atggccgcgt tgatcggtcc gagcgtcgaa gaagtcacgt
     1441 cgatctgcga ggaagcggcg cagggggagg tcgtctcgat cgccaacatc aacgctccgg
     1501 gccagatcgt catcgccgga acgaaagccg gcatcgaccg ggccatcgcc gttgccaagg
     1561 ctcgcggcgt gcgccgcgcc ttgccgctcc cggtctccgc gccatttcac tgcgcgctga
     1621 tgaagccggc cgaagagcga ttgaagccat tgctcgacga cgcgcccttc aaagatctgt
     1681 ggatgtcgct ggtgtcgaat gtcgacgcct cgccgatcgg gacctcgacc gcggtccgca
     1741 acgcgctcgt ccgtcaggtg gtctcgcccg tgcgctgggt cgagtccgtg cagaagatgg
     1801 tatcgatggg cgtccggcgc tttgtcgaga tcggcccggg cgcggtgctc accggtctca
     1861 tcaaacgcat cgatccatcc gttgagttga tcaacgtctc ggatgtgccg tcgatcgaga
     1921 agttcctgga gagcatgcaa tgaaaatcga tctcaccgga cgcaccgctc tcgtcaccgg
     1981 cgcctcccgc ggcatcggcg aagcgatcgc gctgcggctt gccgagtccg gcgcgcatgt
     2041 gctctgtgcg gcgcgaaacc tcgagcacgt cgagcgcgtg gcaggcgtca tcaccgcgaa
     2101 cggcggcagc gcggccgccg tgcagctcga catcacgagc gacgacattc gcgagcgcat
     2161 caaagcgctc atcgaacagc agccgatcga cattctcgtc aacaacgccg gcatcaccga
     2221 cgacgatctc ttcatccgga tgaagccgga ggcgtggacg agtgtcttgc ggacgaacct
     2281 cgactccgcg tttcacatca cccaggaagt ggtgaagaag atgatccgcg cgcggtgggg
     2341 ccgcgtcatc aacatctcct cgatcgtcgg actgtcgggc aacccgggcc aggtgaacta
     2401 cgcgtcgtcg aaggcggcgc tgatcggttt cacgaaagcg ctggcgctcg agatcggctc
     2461 gcgcagtgta accgtgaacg cgatcgcccc cggcttcgtg aagaccgcca tgaccgatgc
     2521 catgaccgat gacgcccgca aaaccctcga agcccgcatc gccctccacc gcctcggcac
     2581 cgtcgacgac atcgcctacg cggccgtttt tctagcctcg gagcaggcgg gctacatcac
     2641 cgggactgtg atgaaggtga gcggcgggct gtatacgtag tacgcgggag tcgggggtcg
     2701 ggggtcgggg gtcggagaag caccggtagt ttccggcaaa cgaatggaac ctggtggcgt
     2761 tatcagcgcg acaggtcggt cattgcacgc gggag
//
LOCUS       DATLVW010000140         6916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_87747, whole genome shotgun sequence.
ACCESSION   DATLVW010000140 DATLVW010000000
VERSION     DATLVW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87747"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..930)
                     /locus_tag="VNN25_04595"
     CDS             complement(25..930)
                     /locus_tag="VNN25_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXH90839.1"
                     /translation="MRDLEIKRILVPTDLSEQSIVSLQYARFFAERFSSSLTLLYVDP
                     LVFPISDAGADIPLYIATTPGHIAKLEKEVRTYAGAALLGYPYDVAAAVGEPVPAIVR
                     EEQERAADLVIMATHGLRGWRRLVLGSVTEGVLRGGDSPVLSVSRSGERLRTPDPVKK
                     ILCPINFTETARASLEYASHLAANFDCELVIVHVVEEGVKTKPIAAGGVRSWIDPAIQ
                     NRCSFREIVQRGGAAERVLDCAEDLGADLIVIGAQHKRFRDETVIGTTTERLVRFARV
                     PVLSVPRRVEVKKTRDEVRAAHVYR"
     gene            complement(1062..2195)
                     /locus_tag="VNN25_04600"
     CDS             complement(1062..2195)
                     /locus_tag="VNN25_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH90840.1"
                     /translation="MTSSEAHPIVMWELTRACDMHCRNCSIGATGSPGANELTTYEVY
                     KTIDQIDVLAPREFIITGGDPLAREDIVQIVNYARRRGLDPALVVSPTHQLTADAITR
                     LERNGLTRIIFNIDGSSPAIHDGVKGTAGTFAITLHAMRWAEQAGLSIEVNTLITRHN
                     AGDLRAIADLIRPIGIKRWNLYFLVPITASYQSAMLTAAEAESLFETIDEIQGKETFA
                     VRVVEAPHYRRYLLQRNLQTRIDNAEVGSWEDFRGYETGDDHPQRDLMDSAVDNARGF
                     VFISHAGDVRASEFLLASAGNLRYRPLSQIYRGSDLFVALRDPANLTGKCGRCEFRHI
                     CGGSRARAWVTEGNIFADDPLCDYEPGVAMPLHAAAHGHAAAS"
     gene            complement(2203..3099)
                     /locus_tag="VNN25_04605"
     CDS             complement(2203..3099)
                     /locus_tag="VNN25_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXH90841.1"
                     /translation="MTEKLNCILVPSDFSEPALHALRYAGKLARELNARLVLLYADPF
                     VPPVDVSATVGGWDDETFEYVKLCAEEQLRIDARATLDPAVSYDVVVRVATPLAGILE
                     QAHESSAGLIVMGTHGRSGFKRLIIGSVTEAVMRHAEVPVIAVPPRGVATPSIKTVVC
                     PVIYKAQCRDALTFAAEIAPPDARFIIISANPNDEVEPADYLAALRAWIPEAIAARCD
                     VRTFGDEHAVNPIEGFAKSTHADLIVAAEPAIRTTADVLRGTFAARLLQHSECPVLTV
                     NAPTAKIAARYAHQEAPAMAHQ"
     gene            complement(3625..4308)
                     /locus_tag="VNN25_04610"
     CDS             complement(3625..4308)
                     /locus_tag="VNN25_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH90842.1"
                     /translation="MPRRVLVVEDDAVSRQLLKRLLEVEGHEAETASGYFEAVKLLDR
                     QRYDLILLDLTLEDGDGLVLCRRIRARDQTPIMIITSRGAASDVVTGLELGADDYVVK
                     PFDIDVLTARIRAQLRRAAETRRGEALDQIRIGRLVIDNGIRDAIVEGIPAGLTNKEF
                     ELLMLLARSQGRAVAKEDILAHLFEGEVRSEKILAVYIRRLRQKIEMDPDKPTILHTV
                     RGFGYRLGD"
     gene            complement(4359..5012)
                     /locus_tag="VNN25_04615"
     CDS             complement(4359..5012)
                     /locus_tag="VNN25_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXH90843.1"
                     /translation="MIHAASDEVLERLWYATEEARTVMSPDELHLDAEDVDLNAVLEA
                     LIAEGDVQCSTGYCLTQAGAQRARGIVRRHRLAEVLFIQAFGTNLRDAEVSACEMEHM
                     LSEPVVERVCAFLGHPPVCPHGKPVPAGECCRVFSSSVQPLIRRATELPVGATATIAF
                     IAPKTLSRIDRLAAFGIVPGSEIRLIARNPSCVIGCGSSSIALDDDIGGEIYVRSVA"
     gene            complement(5193..>6916)
                     /locus_tag="VNN25_04620"
     CDS             complement(5193..>6916)
                     /locus_tag="VNN25_04620"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HXH90844.1"
                     /translation="QLSEYAVPFVVALNMADELRSMDEGPDVVALSQAVGVPFVSVSA
                     LRRWNIGKLKLTLESARRGSMAVSYPMAVEVGIAAVREVLALHAASIANRGVALAILA
                     GDVTLRPLLTASCPAPVLAKLDDLRAAVQSQLARPLSTLISHSRVATVDELLATMQRK
                     SASHVDGLRDRIGRTCMHSIYGVPILLGVLALAWLLVGKIGAGTLVDLMQKHVFEHGI
                     NPWTIRLIDWLAPFPHKHVITDGVITSAYAGHASGFWGNVARTIHDLLVGPYGVVTMA
                     ITYAIAIVLPVVVTFFTFFGLLEDSGYLPRLAVILNRGFRKIGLNGKAVLPMVLGLGC
                     DTMATLTTRILDSKKEATIVTLLLALGVPCSAQLGVILAMLAPLGITATLIWLLVTLL
                     TIVLAGWAASRVVPGEPGDFVLEIPPMRVPRLSNIAIKVAARTEWYLKEAVPLFIVGT
                     LLLYGLDATGMLKYIQRMLGPLIIRGLGLPAEATNAFVIGFLRRDYGAAGLYAMQRAG
                     HLTPNQVVIALTVITLFIPCIANLMVMLKERGARMGWAIAGFIIVYSFGIGVVMNAAF
                     KVLGVVL"
BASE COUNT         1301 a         2168 c         2256 g         1191 t
ORIGIN      
        1 tgtgtctctg tggttcaaaa gggtttatct ataaacgtgc gcggcacgca cctcgtcgcg
       61 cgtcttcttc acttccacgc gcctcggcac cgacaacacc ggcacgcgcg cgaagcggac
      121 caggcgctcg gtggtcgttc cgatcacggt ctcgtcgcgg aagcgtttgt gttgcgcgcc
      181 gatgacgatc aggtccgcgc cgaggtcctc ggcgcagtcg aggacgcgct cggcggcacc
      241 gccgcgttgc acgatctcgc ggaagctgca tcgattctgg atcgcgggat cgatccagct
      301 tcgaacgccg ccagcggcga tcggtttcgt tttgacgcct tcttcgacga cgtgaacgat
      361 gaccagctcg cagtcgaagt tcgcggcgag atgagacgcg tactcgagtg atgcacgcgc
      421 cgtctccgtg aagttgatcg ggcagaggat cttcttcacc ggatcgggag tgcgaagacg
      481 ctcaccgctc cggctgacgc tcagcaccgg gctgtctccg ccgcggagca cgccctcggt
      541 gaccgatccg aggacgagac ggcgccagcc acggagtccg tgcgtggcca tgatcacgag
      601 atcggccgcg cgttcctgct cctcgcgaac gatcgccgga accggctcgc cgacggctgc
      661 cgcgacgtcg tagggatagc cgaggagcgc ggcaccggcg taggtgcgca cctccttctc
      721 cagcttcgca atgtgcccgg gcgttgtggc gatgtagagc ggaatgtccg cgccggcatc
      781 gctgatcggg aacaccagcg gatcgacgta gagcagcgtc agcgacgagg agaagcgctc
      841 cgcgaagaac cgcgcatatt gcagcgagac gatcgactgc tcgctgagat cggtcgggac
      901 gagaattcgt ttgatttcga gatcacgcat gatgccctcc tgaagttcgc ggtttttcct
      961 atcgtcagca tcggcccgac taccgacggc gtcggtgacg aaggaacggc gtccgtgtga
     1021 ctgcgcatac atcgcagccg ttcgcgcccg cgcgaaaacc tctacgacgc tgctgcgtga
     1081 ccatgcgccg cggcgtggag cggcatggcg acgcccggct cgtaatcgca cagcgggtcg
     1141 tcggcgaaaa tgttcccctc ggtcacccag gctcttgcac gggatccgcc gcagatgtgg
     1201 cggaactcgc agcggccgca tttgccggtc agattggcgg gatcgcgcag ggcgacgaag
     1261 agatcgctgc cgcgatagat ctggctgagc gggcgatagc ggaggtttcc ggcgctggcg
     1321 agaaggaact cgctggcgcg gacgtcgccg gcgtgcgaga tgaagacgaa gccgcgggcg
     1381 ttgtcgaccg ccgagtccat gaggtcgcgc tgcggatgat cgtcgccggt ctcgtacccg
     1441 cggaaatcct cccagcttcc gacctcggcg ttgtcgatgc gcgtctgcag attgcgctgc
     1501 aaaaggtagc gccggtagtg aggcgcttcg acgacacgga cagcgaaggt ctccttgccc
     1561 tggatctcgt cgatggtttc gaagagactc tccgcctcgg cggcagtgag catcgccgac
     1621 tgatacgacg ccgtgatggg cacgaggaag tagaggttcc agcgcttgat gccgatcggg
     1681 cggatcaaat cggcgatggc acgcaggtcg ccggcgttgt ggcgcgtgat gagcgtgttg
     1741 acctcgatgg agaggccggc ctgctcggcc catcgcatcg cgtggagcgt gatcgcgaag
     1801 gttcctgccg tccccttgac cccgtcgtgg atcgccggcg acgaaccgtc gatattgaag
     1861 atgatgcgcg tgagtccgtt gcgctccaac cgcgtgatgg cgtccgccgt caattgatgg
     1921 gtcgggctga ccacgagcgc cggatcgaga ccgcggcgcc gcgcgtagtt gacgatctgg
     1981 acgatgtctt cacgcgccag cggatcgcct ccggtgatga tgaattcgcg cggggcgaga
     2041 acgtcgatct ggtcgatcgt cttgtagacc tcgtacgtcg tcagttcgtt cgcgcccgga
     2101 ctgccggtgg cgccgatgga gcaattgcgg cagtgcatgt cgcacgcgcg cgtgagctcc
     2161 cacatcacga tgggatgggc ttcgctgctg gtcatgagca cctcattgat gggccatcgc
     2221 cggcgcttcc tggtgtgcat agcgcgcagc aatcttcgcc gtgggagcgt ttaccgtcag
     2281 aacgggacat tcgctgtgct gcagcagacg cgcggcgaac gtgccgcgca gtacatccgc
     2341 ggtcgtccgg atggccggct ccgcggctac gatcagatcg gcgtgcgtgc ttttcgcaaa
     2401 gccctcgatc gggtttacgg catgctcatc gccgaacgtc cgcacgtcgc agcgcgcggc
     2461 gatcgcttcc ggtatccagg cgcgcaacgc ggccagatag tccgcgggct ccacctcgtc
     2521 attcggattg gcactgatga tgatgaaccg cgcatcgggc ggcgcgatct ccgccgcgaa
     2581 ggtcagcgca tcgcggcact gagccttgta gatgaccggg cagacgaccg tcttgatcga
     2641 tggcgtcgcg acaccgcgcg gcggtacggc gatcaccggt acctccgcat gccgcatcac
     2701 cgcctcggtg accgatccga tgatcagccg cttgaacccg ctgcggccat gcgtacccat
     2761 gacgatcagt ccggcggacg actcgtgcgc ctgctcgaga atgccggcaa gcggcgttgc
     2821 aacgcgcacg acgacgtcat acgaaacagc gggatcgaga gtcgccctgg cgtcgatgcg
     2881 cagttgctcc tccgcgcaaa gtttcacgta ctcgaaggtt tcgtcatccc agccgccaac
     2941 agtggcgctg acatcgaccg gcgggacgaa gggatcggca tagagcagaa cgagccgggc
     3001 gttgagctct cgcgcgagct tgccggcata acggagcgcg tggagggccg gctcgctgaa
     3061 gtccgaggga acgagaatgc agttgagttt ctccgtcatc atggccctcc cgctggctcc
     3121 ttggtgacga tagaagggag gcgcaagccc ggcgatgacc ggggtcattc gggggtgtga
     3181 tggcggtcac gtgggggaat ggttgggcgg cggacggggg gagccgggcc ggggtggctc
     3241 gtgtcaccac cgtgtcatcg cttccggcac ccaccatcgt gtcatccgta ggatgacacc
     3301 aaggccggcg ctgcggtgct aactatcgct aaaataacga tatagagatg agcgaacaac
     3361 ctccagattc tggttaacaa caaccagatt ctggatgaag gagaccagat tctggtcaac
     3421 aacaaccaga ttctggttga agaagaccag attctggttg aagacaacca gattctggct
     3481 gaagacatcg ggcatcgaac caacctgggc gaaccacttt tgacgatccc ttcacggcca
     3541 gcagcgtttt ccgggcttcg acctccgccc tgacagcact cgggcgagga cgcccgagtg
     3601 cccgcggtcg agacgaccgc actcctagtc tccgaggcgg tagccgaagc cgcggacggt
     3661 gtgcaggatt gtgggtttgt cggggtccat ttcgattttc tggcgaaggc ggcggatgta
     3721 gacggccagg atcttttccg agcggacttc gccctcgaag agatgcgcga ggatgtcctc
     3781 cttcgccacc gcccgtccct gactccgcgc caggagcatc aggagctcga actccttgtt
     3841 cgtcaggccg gcaggaatcc cttcgacgat ggcgtcgcga atgccgttgt cgatcacgag
     3901 gcgcccgatg cggatctgat ccaacgcttc gccgcggcgt gtctcggccg cgcgccgcaa
     3961 ctgcgcgcga atccgtgctg tcagcacatc gatgtcgaat ggcttgacga catagtcgtc
     4021 agcaccgagc tcgaggccgg tcaccacgtc gctggcagcg ccgcgggacg tgatgatcat
     4081 gatgggcgtc tgatcgcgcg cccggatgcg gcggcagaga accaacccat cgccatcctc
     4141 gagcgtcagg tcgagcagaa tgaggtcgta gcgctggcga tcgagcaact tcaccgcttc
     4201 gaaatagccg gacgccgtct ccgcttcgtg cccttcgacc tccagaagcc tcttcaacaa
     4261 ctggcgcgaa acagcgtcat cttcgactac cagcacccgc cgtggcatcg tgacaatttt
     4321 agcgccgcat tcatcatcca aagcattcgc acgcggcatc aagccacgct ccgaacgtag
     4381 atctcccctc cgatgtcgtc gtcgagcgcg atcgagctcg agccgcagcc gatcacgcag
     4441 ctcgggttcc gcgcgatgag ccggatctcg ctgcctggaa cgattccgaa tgccgccagg
     4501 cggtcgatgc gagagagagt cttcggcgcg atgaatgcga tcgtcgcggt cgcgccgacc
     4561 ggcagctcgg ttgcgcgccg gatcagcggc tgcacgctgc tcgaaaagac gcggcagcac
     4621 tcccccgctg ggaccggctt gccgtgcggg cagacaggag gatggccgag aaatgcgcag
     4681 acgcgttcga cgaccggctc ggagagcatg tgctccatct cgcacgccga gacttcggcg
     4741 tcgcgcaggt tcgttccgaa ggcctggatg aagagcactt ccgcgagccg gtgccggcgc
     4801 acgatgccgc gcgcgcgctg cgcgcctgcc tgcgtgaggc aatagccggt gctgcactga
     4861 acgtctccct ccgcgatcag cgcctcgagc acggcgttga gatcgacatc ctccgcgtcg
     4921 aggtgaagct cgtccggcga catcacggtt cgcgcttcct ccgtcgcata ccagagccgt
     4981 tcgagcactt catcggatgc tgcgtgaatc atgggacctc cgtttgaaca gtgacgtcag
     5041 caagcgcgta atgcggccgc tgccgacaaa ctcatagaaa cagcgcgggc aacgggacat
     5101 ctcgcatccg cgcgagatcg gacacgacga atgacacccc gaagcgacga aatcggtgcc
     5161 gcacatcgga cactccagat ggacgtcatc gttcacagca cgactccaag aaccttgaat
     5221 gcggcgttca tcacgacgcc gatgccgaat gagtagacga tgatgaagcc ggcgatcgcc
     5281 caacccatcc gcgcgccgcg ctccttgagc atgaccatca ggttggcgat gcacggaatg
     5341 aagagcgtga tgacggtcag cgcgatcacg acctggttgg gcgtgaggtg gccggcgcgc
     5401 tgcatcgcgt agagaccggc ggcaccgtag tcgcggcgca ggaatccgat cacgaacgcg
     5461 ttcgtcgctt cggccggcaa tcccagtccg cggatgatga gcggcccgag catccgctgg
     5521 atgtatttca gcatgccggt ggcgtcgagg ccgtacagca gcagcgtgcc gacgatgaag
     5581 agcggaacgg cttcctttaa gtaccactcc gtccgcgccg cgactttgat ggcgatgttc
     5641 gacagacgcg gaacgcgcat cggcggaatc tcgagaacga aatcgcccgg ctcgcccgga
     5701 acgacgcgcg acgccgccca tcccgccagc acgatcgtca gcaacgtcac cagcaaccag
     5761 atcagcgttg ccgtgatccc gaggggcgcg agcatcgcca ggatcacacc gagttgggcg
     5821 gagcatggga cgccgagtgc gaggagaagc gtgacgatgg tcgcttcctt ctttgagtcg
     5881 aggatgcgcg tcgtcagcgt ggccatcgtg tcgcatccga ggccgaggac catcgggagg
     5941 acggccttgc cattgaggcc gatcttgcgg aaaccgcgat tgaggatcac cgcaaggcgc
     6001 ggcaaatagc cggagtcttc cagcaacccg aagaacgtga agaaggtgac gaccaccggc
     6061 agcacgatcg cgatcgcgta cgtgatcgcc atcgtcacga cgccgtacgg gccgacgaga
     6121 agatcgtgaa tcgtgcgcgc gacgtttccc cagaatccgg acgcgtgacc cgcgtatgcc
     6181 gaggtgatca cgccatcggt gatgacatgt ttgtgcggaa acggcgcgag ccagtcgatc
     6241 aggcggatcg tccacggatt gatcccatgt tcgaacacat gcttctgcat gagatcgacg
     6301 agcgtgccgg cgccgatctt tccgacgagc agccaggcca gggcgagaac gccgagcaaa
     6361 atcgggacgc cgtagatgga gtgcatgcag gtgcgtccga tgcgatcgcg gagaccatcg
     6421 acatgcgagg cgctctttcg ctgcatcgtg gccagcagtt cgtcgaccgt tgcgacgcgt
     6481 gaatgcgaga tcaacgtgct gagcggacgg gcgagctgac tctgcaccgc cgcgcgaagg
     6541 tcatcgagct tcgcgagcac gggcgcggga cacgaggcgg tgagaagcgg ccgcaaggtg
     6601 acgtcgcccg cgaggatcgc aagcgccaca ccgcggttgg caatggacgc cgcgtgcaat
     6661 gccagcacct cacgaaccgc ggcgattccc acttccaccg ccatcggata cgacaccgcc
     6721 atcgatcctc ggcgtgcgct ctccagcgtc agcttgagct tcccgatgtt ccaacgccgc
     6781 agcgcggaga cggaaacgaa cggcacgccc acggcctgcg agagcgcgac gacgtccgga
     6841 ccttcatcca tcgagcgcag ttcgtccgcc atattgagcg cgacgacgaa cggcaccgcg
     6901 tactcgctga gctgca
//
LOCUS       DATLVW010000141         8267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_5194, whole genome shotgun sequence.
ACCESSION   DATLVW010000141 DATLVW010000000
VERSION     DATLVW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5194"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1874
                     /locus_tag="VNN25_04625"
     CDS             <1..1874
                     /locus_tag="VNN25_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXH90845.1"
                     /translation="LRTPAARAQRRITTAKLPWMLAVATTLLVPVVMSLRGQDPFRLP
                     KNLLFLAFAIAIAAAAIIVWLRGTGRDQQSVAVRTVPLIAIGSVCWTALASLFSTNHA
                     LSYQAILWVAAVAIFACAVDLGGRTRGTLAMAWPLIPAAINTVLFLLQRFHVWNPMTF
                     PADLPDHMRYTALLGNPDDVGGLLVAPALVAVALVLCDRRRRALWIPAATLLLAGIAT
                     GRLTSLIACGAGLLVMGFVRSRRVGAIATALLVVGGIALVVGDPPTRARASSIANALR
                     AHDYAEATSGRITAFVSASMMAMDHPLFGVGPGCFRWAYFEYKVAAERAHPSLAKAWS
                     ADYNFGEAHNDHLQTLAESGLPGYALFVAALVALAASSRRKKNSESEDETERGTLVRT
                     LALPTSVAVAILCLAHFPLHLAASTVTIVYIATLCVSWGAHAEIKVPPALNERLAAIL
                     RPRRLSPALSIAFVIVVVIGAAFLITRIALQPWQCNLTEEEIQTRTSLTYDSVTDPVQ
                     TSRNAHQNLAALETCLDVSPHDIALYMLAAANQRLIGRDADAATMYRRALQYDRRPEL
                     YYNLGVVELQLRQRDAAIADLLTAVSFSRAYLHDLPADVQAEVSDALRRQYPYLAGS"
     gene            complement(1828..2445)
                     /locus_tag="VNN25_04630"
     CDS             complement(1828..2445)
                     /locus_tag="VNN25_04630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXH90846.1"
                     /translation="MLRAFDVAVSLVAIVFLSPLFAVIAVAVKLTSPGPLFYRGPRVG
                     RFGAEFSILKFRTMRAGSAGPSITRAGDPRVTPVGRFLRLWKLDELPQFVNVLRGEMS
                     LVGPRPEAPEYVLLYTPEERNVLRVRPGITSPASLHYRDEESLLTGQAWRDEYVNRIM
                     RDKLRIDLEWLDRRTFASDLRLLARTAASIFSSRRDTDTAAAERH"
     gene            complement(2445..3599)
                     /locus_tag="VNN25_04635"
     CDS             complement(2445..3599)
                     /locus_tag="VNN25_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HXH90847.1"
                     /translation="MTFIPFARPDLGAAEMDAVRAVLESGWLTTGERVHEFEAAFAAA
                     VGAKHAVALNSCTAALHLALEAIGLRAGDEVIIPAYTFTATAEVVLYFGATPVLVDID
                     ARTLNIAPRRVAEAMTVRTKAIIPVHFGGLAADLDALRAVAEPRDISIIDDAAHAFPS
                     RYNGKNIGAISDITCFSFYATKTITTGEGGMLCTDDDAYAARCRLMALHGISHDAWKR
                     YRAEGSWYYEVVAPGFKYNMTDIAAALGLAQLRRADAMRARRQEIAERYNAAFEGHEF
                     ELPAFGDAREHAWHLYPLRLPRGADRGAFVDALRARGIGASVHFIPLHLHPFYRDRYG
                     FTPDSFPVSIAEYEREVSLPIYSAMSDAEVGEVIGAVTAVIRGASRVLAG"
     gene            complement(3596..4198)
                     /locus_tag="VNN25_04640"
     CDS             complement(3596..4198)
                     /locus_tag="VNN25_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH90848.1"
                     /translation="MIVRSMARGDLAGVVAIHLAAFPNFFLSFLGPRFLHVFYGFIAG
                     EGIALVALADGQLAGFVAGVLEPRLFYRRLMRRRFAQLALAITPTVLRHPSTLARVTR
                     RGRQRTSAGNHEPAGAELMSLAVEPGQDHRGIGRALVAAFAMHVAASGERRLWLTTDA
                     ADNDRVIHFYESQGFRLSREFVTAEGRAMVELTRDVEPPS"
     gene            complement(4195..5409)
                     /locus_tag="VNN25_04645"
     CDS             complement(4195..5409)
                     /locus_tag="VNN25_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH90849.1"
                     /translation="MASRQQPRALGAPTIRVWLLNPYGPIPGEGWRDYRFTLMARALA
                     ERGHEVTWFTATFAHQSKQQRAAGWERREVSERFAIELVPTPSYRRNIGVARLWFEQL
                     FARRVNKRGRALSHPDVILAADPPQFCGAAGRRLARHHHAFFVTDCFDLWPELFVAAA
                     PYSLRFLVNAAVAPLRKLRRRNLRATSLAIAVAETYRRVLEREGARRTVTIPIGIDVN
                     AFAPHEKRARDHVALVYAGSLGEHYDLDTLLDVLATMPKVRLTIAGRGPAEERLRRRS
                     ASLPNVFFAGAVGVEKLSELYAASDVGLAPYQAGSTVALPTKLFDYLAAGLPVITSLD
                     SEVSTLIEDAHAGVRYKAGDAASLRAAIVALSDPSRRGPMATAARELAMRFDARPLYA
                     QFADAIEALVRS"
     gene            complement(5409..6443)
                     /locus_tag="VNN25_04650"
     CDS             complement(5409..6443)
                     /locus_tag="VNN25_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXH90850.1"
                     /translation="MENQGLEIDWRARLRHAWPQIGIGAVIGIGTLMLAVRGVDWPAV
                     RSALLHTRGSMILLALLSVAGAISLSVVRWRLLFFPAHRQLSWRGLAGAIVIGQTANI
                     VIPARVGELGRIYLVGSREGVSKAHVTATIVVEKVADLAVFAVAIGLALVGMAMPEWM
                     SRSGVAFVAVAAVLVVATVALTFWSESLLRFLERLAERLLPAKWARRTVGVADAALEG
                     LRSLRDWRLGLGVWLLSGAIFFLSVTNNYLVFKAMRLPLSPVAALFLTIVLRIGVAPP
                     SLPGRLGLFQYLVVLALAMFSVNRTAALTYSFALYGTAVLPVLFAGVVSLFAIRWTAR
                     SLGSIAGREV"
     gene            complement(6637..7878)
                     /locus_tag="VNN25_04655"
     CDS             complement(6637..7878)
                     /locus_tag="VNN25_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXH90851.1"
                     /translation="MPDTSSPRPALPRFVWFELFLVLILAPLLAFPPLGHPWLLFVAF
                     LLPVLWLIKPLPITPLNGSLLLLVASVLLSLTASFDIAYSTGKVFGVVLGAAIFFAVV
                     RAIRSRRVLDFAIETYAAAGGALAAIGILGTNWIGKIGALQSLTAHVPAVIRGVPGQQ
                     EGFQPNAIAGALLFFIPLQLALVISGRRRVLAVLLLLFTSGVFLLTQSRNGWLSLAVA
                     MGAWAIWQSRGRLRRVFVGAVIVVAIVSAAFWTYGGSRLSERVVGGGLSNDVESRVEL
                     WSRAIYMIEDFPFTGIGMNNFRRVMPVMYPVFLTPPDYDVAHAHNQLLNTGAELGVPG
                     LVAYIAILLGVATMLVRTSRTTTDARLRWIAAGLGAGFLAFFGFGMADAIALGAKVGI
                     VFWAALALVVAVDACSREMSS"
     gene            complement(7928..>8267)
                     /locus_tag="VNN25_04660"
     CDS             complement(7928..>8267)
                     /locus_tag="VNN25_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HXH90852.1"
                     /translation="ALRGGAPSNVSVFAGRIADTGRDPMPIMAESVRILADVPGAELI
                     WASPRELLNVFQADEIGCHIITATNDILKKLDLIGRDLADYSLDTVKMFFNDAAQAGF
                     ALDVTAAAPR"
BASE COUNT         1717 a         2705 c         2519 g         1326 t
ORIGIN      
        1 ggctccgtac tcccgcggcg cgtgcgcaac gtcgcatcac gacagcgaaa ctgccgtgga
       61 tgctggcggt cgccacgacg ctgctcgtac cggtcgtgat gagcttgcgt ggccaagacc
      121 cgttccggtt gccgaagaat ctgctcttcc tcgcattcgc catcgccatc gcggccgctg
      181 cgattattgt ctggctgcga ggcaccgggc gcgaccaaca gtcagtcgca gtacgcaccg
      241 tgccgctgat cgcaatcgga agtgtgtgct ggacggcgct tgcgtcgctc ttcagtacca
      301 accacgcact cagctaccaa gcgatcctct gggttgcagc cgtcgccatc ttcgcctgtg
      361 ccgtcgacct cggcggccgc acccgtggca cgcttgcaat ggcatggccc ctgattcccg
      421 cagccatcaa cactgttctc tttctgctcc agcgtttcca cgtgtggaat ccgatgacgt
      481 ttccggccga cctgccggat cacatgcgct acacggccct tctggggaac cctgatgacg
      541 tcggcggtct gctcgtagcg ccggcgctgg tcgcggtcgc gctggttctt tgcgaccgac
      601 gccggcgcgc gctctggatt cctgcggcga cgctcctgct ggccggcatc gcaacggggc
      661 ggctgacttc gctcatcgcg tgcggcgcgg gactgctggt gatgggattt gtgcgatcgc
      721 gccgagtcgg agcgatcgca accgcattgc tcgtggtcgg gggaattgcc ctcgtcgttg
      781 gcgatccgcc gacgcgcgca cgcgcctcga gcatcgccaa cgcattgcgc gctcacgact
      841 acgccgaagc aacctccggc cggattacgg cgtttgtttc agcgtcgatg atggcgatgg
      901 atcatccgct cttcggagtc gggccaggtt gtttccgctg ggcatacttc gaatacaaag
      961 tggccgcgga gcgcgcccat ccgtcgctgg cgaaagcgtg gagtgcagat tacaacttcg
     1021 gcgaagccca taacgatcat ctgcagactc tcgccgagag cggcctgccc ggttacgcgc
     1081 tcttcgtcgc cgcgctggtc gcactggccg cctcatcccg tcgcaagaaa aacagcgaaa
     1141 gcgaagatga aacggagcgc ggcaccctcg ttcgtacgct ggcacttccg acgagcgtgg
     1201 ctgtcgccat tctctgtctc gcacactttc ctctgcacct cgccgcgtcg acggttacga
     1261 tcgtctacat cgccacactc tgcgtgtcat ggggtgcaca tgcggagatc aaggttcctc
     1321 cggcattgaa cgagcgcctc gcggcaatcc tccgtccgcg tcgtttatca cctgctctct
     1381 cgatagcttt cgtcattgtg gtcgtaatcg gcgcagcatt cctgatcact cgaatcgcgc
     1441 tccaaccctg gcagtgcaat ctcacagagg aggagattca gacacggacg agtctgacct
     1501 acgacagtgt gaccgacccg gtacagacgt cccgcaacgc gcaccagaac cttgccgcgc
     1561 tggagacgtg cctcgacgta tcgccccacg acatcgctct ctacatgctc gccgcggcaa
     1621 accagaggct gatcggccgc gatgcggatg cggcgacgat gtaccgccgc gcgctgcaat
     1681 acgaccggcg tccggagctg tactacaacc tcggcgtcgt ggagctccag ctgcgccaga
     1741 gggatgctgc gatcgccgac ttgttgacgg ccgtgtcgtt cagccgcgcg tatctgcacg
     1801 atctgccggc tgatgtgcag gccgaggtta gtgacgctct gcggcggcag tatccgtatc
     1861 tcgccgggag ctgaagatcg acgccgcggt gcgagcgagg agacgaagat cggacgcgaa
     1921 cgtccggcga tcgagccact cgagatcgat gcgtagtttg tcgcgcatga ttcgattcac
     1981 gtactcgtcg cgccacgctt ggcccgtcaa cagagactcc tcgtcgcgat aatgaagcga
     2041 tgccggactg gtgatgccgg gccgcacgcg cagcacgttc cgctcctccg gagtgtaaag
     2101 cagcacatat tcaggcgctt caggacgtgg ccccacgagg ctcatctcgc cgcgcagaac
     2161 attcacaaat tgcggcaact cgtcgagctt ccataatcgc aggaaacgtc ctacaggcgt
     2221 gactctcgga tcgcctgcac gcgtaatcga cggtcccgcg gagccggctc tcatcgttcg
     2281 gaacttcaag atcgagaatt ccgcgccgaa gcggcccacc cgaggcccgc ggtaaaaaag
     2341 ggggcctggc gaggtgagct tcactgccac cgcgatcacc gcgaaaagag gtgacaggaa
     2401 gacgatggcg acgagcgaga cggcgacgtc gaacgcgcgc agcatcaccc ggccagaacg
     2461 cgcgacgcgc cgcgaatcac ggccgtcacc gcgccgatca cctcgcccac ctcggcatca
     2521 ctcatcgccg agtagatcgg aagcgagact tcgcgctcgt attcagcgat gctgaccggg
     2581 aaggaatcgg gcgtgaaacc gtagcggtcg cggtaaaaag gatgaagatg cagcgggatg
     2641 aagtgcacgc tcgcgccgat tccccgcgcg cgcaacgcat cgacgaatgc tccgcggtca
     2701 gcacctcggg gaaggcgcaa cggataaaga tgccacgcgt gttcgcgagc gtcgccgaac
     2761 gccggcaact cgaattcatg accttcgaat gcagcgttgt atcgctctgc gatctcctgc
     2821 cgccgcgccc tcatcgcgtc cgctcggcgc agttgtgcga gaccaagcgc ggcggcgatg
     2881 tcggtcatgt tgtacttgaa ccccggagct actacctcgt agtaccacga accttcagcc
     2941 cgataacgct tccatgcatc gtgactgatg ccatgcagcg ccatcagccg gcatcgggcc
     3001 gcgtaggcgt cgtcatcggt gcagagcatg ccgccctcac ccgtcgtgat cgtcttcgtc
     3061 gcgtaaaaac taaagcaggt gatgtcgctg atcgcgccga tgttcttgcc gttgtagcgc
     3121 gacggaaacg cgtgcgcggc atcgtcgatg atggaaatgt cccgcggctc ggcgaccgcg
     3181 cgaagcgcat cgagatcggc agccagtccc ccgaaatgaa cgggaatgat cgccttcgtc
     3241 cggactgtca tcgcctccgc gacgcggcgc ggagcgatgt tcagcgtccg ggcatcgata
     3301 tccaccagca ccggagtcgc gccaaaatag aggacgactt ctgcggtagc ggtgaacgtg
     3361 taggccggaa taatgacttc gtcgccggcg cgaagaccga tggcttcgag agcgaggtgc
     3421 agcgccgccg tacacgaatt gagcgcaacc gcgtgtttcg cgccgacagc cgccgcgaac
     3481 gcggcctcga actcgtgcac gcgctccccg gtcgtcaacc agcccgattc gagaaccgcg
     3541 cggacggcat ccatttccgc tgcgcccaga tccggccgtg caaaggggat gaaggtcacg
     3601 atggcggctc cacgtcccga gtgagctcca ccattgcacg cccctcggcg gtgacaaact
     3661 cacgcgacag ccggaatccc tgactctcgt aaaagtggat cacgcgatca ttgtctgcgg
     3721 catcggtggt cagccacaag cgccgctcgc cggaagctgc cacatgcatc gcaaatgcgg
     3781 cgaccagcgc acggccaatc ccgcgatggt cctgtcccgg ctcgaccgca agtgacatca
     3841 attccgcgcc cgccggctcg tgattcccag cactcgttcg ctgacgcccg cgccgcgtga
     3901 cgcgcgcgag ggtcgacgga tgacgcaaca ccgtaggagt gattgccagc gctagttgag
     3961 caaagcgccg cctcatcagc cgccggtaga acagtctcgg ctccagaaca cctgcgacga
     4021 agccagccag ctgcccgtcc gcaagcgcga cgagggcgat gccttcgccg gcgatgaagc
     4081 cgtagaagac atgaagaaaa cgtggtccaa gaaacgacag gaagaagttc gggaaggcgg
     4141 cgaggtgaat tgccacgacg cccgcaaggt ctcctcgcgc catcgagcgc acgatcatga
     4201 gcgcaccagc gcctcgatgg catcggcgaa ttgcgcgtag agcggacgag catcgaatcg
     4261 cattgccaac tcacgcgctg cggttgccat cggtccgcga cgactcggat cggagagcgc
     4321 aacgattgca gcgcgtagag acgcggcatc gccagctttg tagcgaacac cagcgtgggc
     4381 atcttcgatc aacgtgctca cctcgctgtc gagcgaagta atcacgggaa gtccggcggc
     4441 gaggtaatcg aacagcttcg tcggcaacgc aacggtcgac cctgcttggt acggcgcaag
     4501 tccgacgtcc gaagccgcat agagctccga tagtttttcc acaccgaccg cgccggcgaa
     4561 aaacacgttc ggcagcgacg cagaccgcct gcgcaggcgt tcctcggcag gtcctcgccc
     4621 ggctatcgta agcctgacct taggcatggt cgcgagtacg tcgagcaaag tgtcgagatc
     4681 gtagtgttcg cccagactgc ccgcgtaaac cagcgcaacg tgatcgcgag cgcgtttctc
     4741 gtgcggcgca aaggcattca catcgatacc gatcggaatc gtcaccgtgc gccgcgcacc
     4801 ctcgcgttcg aggacccgac ggtacgtctc ggcgacggca atggcgagcg aagtcgcacg
     4861 aagattgcgt cgccgcaact tgcgcaaagg cgcgacagcc gcgttgacga ggaagcgcaa
     4921 cgagtacggc gccgcagcaa cgaacagttc cggccagaga tcgaagcagt ctgtgacgaa
     4981 aaacgcgtga tgatgacgag ccagtctccg tccggctgcg ccacaaaact gtggaggatc
     5041 cgcggcgagg atgacatcag ggtgcgacaa cgcccggccg cgtttattca cgcgccgcgc
     5101 aaacagttgt tcgaaccaga gccgcgcgac gccaatattg cggcgataag acggcgtcgg
     5161 aacgagctcg atggcgaatc gctcgctgac ttcgcgacgc tcccaccccg ccgcccgctg
     5221 ctgtttcgac tgatgagcaa acgtggccgt gaaccaggtt acctcgtggc cgcgttccgc
     5281 aagcgcccgc gccatcagcg tgaatcggta gtcgcgccag ccctcacccg gaatcggccc
     5341 gtacggattg aggagccaga cgcgtatggt tggcgcgccg agcgcccgcg gctgctgccg
     5401 gctagccatc aaacttcccg gcctgcgatg ctcccgagac tccgtgccgt ccaccgaatc
     5461 gcgaagagcg acacgacgcc cgcgaacaga accggaagga ccgccgtgcc gtagagcgca
     5521 aacgaatagg tcaacgcggc agtacgattg acgctgaaca ttgccagcgc cagaacgacc
     5581 aggtactgga acagtcccag acgtcccggc agcgacggcg gcgctactcc gatgcgaagc
     5641 acgatcgtca gaaacagcgc tgcgaccggc gagagtggaa gccgcatcgc tttgaagaca
     5701 aggtagttgt ttgtcacgga caaaaagaag atcgcgccgg agagcagcca cactcccaac
     5761 cccagtcgcc agtcgcgcag cgagcgcaac ccttcgagcg cagcatcagc cacgccgacc
     5821 gtgcgacgcg cccacttcgc cggaagaaga cgctcggcga gacgttccag gaatcgcagc
     5881 agcgattcgc tccagaacgt cagcgcgacc gttgccacaa ccagcaccgc ggccaccgcg
     5941 acgaacgcca caccggaacg cgacatccac tcgggcatag ccattccgac gagggcgaga
     6001 ccgatggcaa ccgcgaacac agccaggtct gcgaccttct cgaccacgat ggtcgccgtc
     6061 acgtgcgcct tgctgactcc ctcgcggctg ccgacgaggt agatccggcc caactcgccg
     6121 acgcgcgccg gaatgacaat gttcgcggtc tgcccgatga caattgcgcc ggccagtccg
     6181 cgccaactga gttgacgatg cgcaggaaag aaaagcagac gccagcgaac aacggatagc
     6241 gaaatggcac cagccaccga gagcagtgcg agcagaatca tgctgccgcg cgtatggagc
     6301 agcgcgctcc gcacggcagg ccaatcgacg ccccgcaccg ccagcatcag cgtgccaatg
     6361 ccgatgaccg ccccgatgcc gatctgcggc caggcgtggc gcaggcgggc gcgccagtcg
     6421 atctctagtc cctggttttc catcgcagta caaacgggag tgcaagccag agaacggctt
     6481 cccaccaccg gcttaccgtt gccacgccca cacaaaacat cgcagcccca acgaacgcgt
     6541 aacgggacac gagcgtgcgg tggaaactcc aagaggcgac gacgatggca gcacctatga
     6601 tccagatgga atgccgcaac acgctccacc aatcgatcac gatgacattt cccggctaca
     6661 cgcgtcaacg gcaacgacga gtgccagtgc cgcccagaaa acgataccga ccttggcgcc
     6721 gagtgcgatc gcgtcagcca ttccgaatcc gaagaacgcc agaaatccgg ccccgagtcc
     6781 tgcggcaatc cagcgcaagc gagcgtcggt cgtcgtgcgc gacgttcgta ccagcatcgt
     6841 ggcaaccccc agcagaatcg cgatgtatgc gacaagccca gggacgccga gctcagcacc
     6901 cgtgttgagc agttggttgt gagcatgggc aacgtcgtag tcgggcgggg tcaggaagac
     6961 cggatacatc accggcatca ctcggcggaa attgttcatg ccgatgccgg taaatggaaa
     7021 atcttcgatc atgtagatgg cgcgcgacca caactcgacg cggctctcca cgtcattcga
     7081 aagaccgcca cccacgacgc gctcgctaag tcgtgatccg ccgtacgtcc agaacgccgc
     7141 gctcacgatg gccacaacga tgacggctcc gacgaagaca cgtcgcagtc gtccgcgact
     7201 ctgccatatc gcccatgctc ccatcgccac cgccagactg agccacccgt ttcgcgactg
     7261 cgtcaaaaga aagacgccag atgtaaagag gagaagcaga acggccagca cgcgccgcct
     7321 tccgctgatt acgagcgcga gctgcagggg aatgaagaag agtaaggctc ctgcgatcgc
     7381 attaggctga aagccttctt gctgccccgg cacgccgcgg attacggccg gtacatgagc
     7441 agtgagactc tgcagcgcgc cgattttgcc gatccagttc gtccccagaa tcccgatcgc
     7501 cgcaagggca ccgccggcag cggcgtacgt ttcgattgcg aagtccagca cgcgtcgcga
     7561 acgaatagcg cgcactacgg caaagaagat cgctgcgccg aggacgacgc cgaagacttt
     7621 gcccgtgctg taggcgatat caaaactcgc cgtcaggctg agaagaacgg acgccactag
     7681 aagcagaagc gatccgttga gaggagtgat gggcagtggc ttgatcagcc acagcaccgg
     7741 aaggaggaag gccacgaaga gcagccacgg atgcccgagg ggcggaaacg ccagcaacgg
     7801 ggcgaggatc agaacaagga aaagctcgaa ccagacgaag cgcgggagtg cgggccgcgg
     7861 actggacgtg tcaggcacgg cggcggtcaa tagcgctccc actcccggcg ccgcgtgact
     7921 gcggccatca tcgcggcgca gccgcggtca cgtcgagtgc aaaccccgcc tgcgcagcgt
     7981 cattgaagaa catctttacc gtgtcgagcg aatagtcggc caggtcgcgg ccgatgagat
     8041 cgagcttctt gaggatgtcg ttcgtggcag tgatgatatg gcagcctatc tcgtcggcct
     8101 ggaagacgtt cagaagctcg cgcgggctgg cccaaatcaa ttccgctccc ggcacatcgg
     8161 cgaggattcg caccgactcc gccatgatcg gcatcggatc gcgtccggta tcggcgatgc
     8221 gacccgcgaa cacagagacg ttgctcggcg ccccgccgcg aagagca
//
LOCUS       DATLVW010000142         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_87822, whole genome shotgun sequence.
ACCESSION   DATLVW010000142 DATLVW010000000
VERSION     DATLVW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87822"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VNN25_04665"
     CDS             complement(<1..1134)
                     /locus_tag="VNN25_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HXH90853.1"
                     /translation="MLRKFLHIGMGFGALLLARLPWRYAALIGVGGIIVNWLLLHRIV
                     GRRVSRHERGWDIGIVLYPFSVTVLIIVFHWHLEIAAVAWVLLAFGDGFSTVIGKRLP
                     LAALPWNPAKSWGGLLAFILFGGAAAFLIARVFGAPHDVAIGAAVLVSAIAESLPLGI
                     DDNVTVPFIAAGVLAAMAIKPIMTLASVPPIAWPWIIVNTVLAILGFALRSVDVSGAV
                     AGWILGCVIVIGGGPPMYVALLAFFVLGTLATKLGYARKARAGLAQEGGGRRGAAHAF
                     ANVGVAAICAVACWRGLGLVPLFMGIAALATAAADTTASEIGQLFGKRAFLPLTLRRV
                     EPGTEGAISLEGTLSGVIAASIVAMAGTALAVHQFRPGFTGNIV"
     gene            1225..2814
                     /locus_tag="VNN25_04670"
     CDS             1225..2814
                     /locus_tag="VNN25_04670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH90854.1"
                     /translation="MTDSPAEQPTDSPAGLTSKPPLLSVVVPCYNERSTVAELLRLVR
                     DVPIDKEIIVVDDKSTDGSAAIVAALAKEWPEIRHVFQPVNQGKGAAIRRGIAEARGE
                     IVLIQDADLEYDPHEYPKLIQPIVDGHADVVFGSRFEGYPRRVMLYWHRLGNNLLTFL
                     SNCTTNLDLTDMETCYKVFRRDVIQSINLKSDRFGIEPEVTAKLARRGYRIFEVPISY
                     YGRDYWEGKKINWKDGFSAIWTIFRYGLFDDPSNEPATYKTLRRRGSLSRYNRWIWEA
                     VAPFVGQRVLEAGAGSGTMTRFLYGRELIVATDKETPYIDRLRNAFRRRPGIKVERLD
                     LDSDAALALAHYNFDTVMCINVLEHTDDDAAALRRVYQLLTPGGRVVIFVPAGKDLFG
                     TLDRGIGHQRRYDQDELLAMMRETGFEIEDSFYQNRAAMLAWRLNSKIFNRGALPAAQ
                     SKIFDRLVPLIKALDGDRPKKGLSLIAIGRKGVGSGGLGVGANADATQPVETGVAGRG
                     SGVGAETTPSTSAASNTAQLP"
     gene            2811..3737
                     /locus_tag="VNN25_04675"
     CDS             2811..3737
                     /locus_tag="VNN25_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HXH90855.1"
                     /translation="MNSAAPDSSANDSSGEHASAIASTPTPHTPLPTPRTTLILAGGA
                     GTRLRPLSSDDNPKQFLRIFDGVSLLEKTVARVGPLVSTESIFISTNDQYAAKCTAFL
                     PELPKGNILTEPARRNTAPAIALCCFAIEAITGGDPVIAVLASDHYIADEAEFVRVLD
                     RAYQFASKNDYLVAIGIEPTEPNTGYGYLQLGDEIEPGITAVERFTEKPSRERAEEFL
                     AAGNYAWNASMFVWRASVFRRALAAAAPEIANVTRENYESMPSISIDYALMEKAPRVA
                     AIRGDFGWSDVGSFEALERVGVPVASLLAKYK"
BASE COUNT          722 a         1222 c         1201 g          622 t
ORIGIN      
        1 gacgatgttg ccggtgaagc ctggacggaa ctgatgcact gccagggccg tccctgccat
       61 cgctacgatc gacgcggcga tgacgccgga cagcgttcct tcgagcgaga tcgccccctc
      121 ggttcccggc tcgacgcggc gtagcgtcag cggaaggaac gcgcgcttgc cgaagagctg
      181 cccgatctcg gacgcagtgg tgtccgcagc ggcagtggcc aatgcggcga ttcccatgaa
      241 cagcggcacg aggccgaggc cacgccagca ggcaacggcg cagatcgcgg ccacgccgac
      301 gttcgcgaac gcgtgtgcgg ctccgcggcg tccgccacct tcctgcgcca gaccggcgcg
      361 agccttgcgc gcgtagccga gctttgtcgc aagcgtgccg aggacgaaga aggcaagcag
      421 cgcgacatac atcggaggac cgccgccgat gacgatgacg catccgagga tccagccggc
      481 cactgcgccc gagacatcga cgctccgtag cgcgaagccg agaatcgcca gcacagtgtt
      541 gacgatgatc catggccagg cgatcggcgg gacgctggcc agcgtcatga tcggcttgat
      601 cgccatggcc gcgagcacgc cggcggcgat gaatggcacc gtgacgttgt cgtcgatgcc
      661 gaggggtagt gattctgcga tggcgctgac caggacggcc gcgccgatgg cgacatcgtg
      721 cggcgcgccg aaaacgcgcg cgatcagaaa tgccgccgca ccgccgaaca ggatgaacgc
      781 gagtaacccg ccccacgact tcgcggggtt ccacggtagc gccgcgagcg gcaggcgttt
      841 accaatcacg gtcgagaatc cgtcgccaaa cgcgagcagc acccacgcca cggccgcaat
      901 ctcgaggtgc cagtggaaga cgatgatcag caccgtcacc gagaacggat agagcacgat
      961 gccgatgtcc cagccgcgct catggcgtga gacacgccgg cccacgatgc ggtgcagcag
     1021 cagccagttg acgatgattc cgccgacgcc gatgagcgcc gcgtaccgcc acggaaggcg
     1081 ggccagcagc agcgcaccga agcccatgcc gatgtgcagg aactttcgca gcatctcgtt
     1141 cgcttcgtgt cgcggctctt ccgcgagccc ggttgacctg cgttgatcga gcatcgtttt
     1201 caacagctta caatacgcgc tccaatgacg gattctcctg ccgaacaacc gaccgactcc
     1261 cctgccggcc tcacgtccaa accgccgctt ctctccgttg tcgttccctg ctacaacgag
     1321 cggtcgacgg tggcggagct gcttcgtctc gtccgcgatg tgcccatcga caaggagatc
     1381 atcgtcgtcg atgataagtc gactgacggc tccgcggcca tcgtcgctgc gttagcaaaa
     1441 gagtggcccg agatccgcca cgtgttccag ccggtgaacc agggcaaggg ggcggcgatc
     1501 cggcgtggga tcgccgaggc gcgcggcgag atcgtgctga tccaggatgc cgacctcgag
     1561 tacgacccgc acgagtatcc gaagctgatt cagccgatcg tcgacggtca cgccgacgtc
     1621 gtcttcggct cccgcttcga aggctaccct cggcgcgtca tgctctactg gcatcgcctc
     1681 ggcaacaacc tcctgacgtt cctctccaat tgcacgacca acctcgatct cacggacatg
     1741 gagacctgct acaaagtttt ccgccgcgat gtgatccagt cgatcaacct gaagtctgat
     1801 cgtttcggca tcgagcccga ggtgacggcg aaactggctc ggcgcgggta ccggatcttc
     1861 gaagtgccga tctcgtacta cggccgcgat tactgggaag ggaagaagat caactggaag
     1921 gatggcttca gcgccatctg gacgatcttc cgctacgggc tctttgacga tccgtcgaac
     1981 gagccggcga cgtacaaaac gttgcgccgg cgcggctcac ttagccgcta caaccgctgg
     2041 atctgggagg ccgttgcacc gttcgtcggg cagcgtgtgc tcgaggcggg tgcgggctcc
     2101 gggacgatga cgcgttttct ctacggacgc gaactgatcg tggccacgga caaggagacg
     2161 ccgtacatcg accgtctgcg caacgcattc cggcggcgtc ccggcatcaa ggtcgagcgg
     2221 ctcgatctcg acagcgatgc cgcactcgct ctcgctcact acaacttcga cacggtgatg
     2281 tgcatcaatg ttctcgagca cacggacgac gatgcagccg cgctgcgccg cgtgtaccaa
     2341 ctgctcactc ccggagggcg cgtggtcatc ttcgtgccgg ccggaaagga tcttttcggc
     2401 acgctggacc gcggcatcgg ccatcagcgc cgttacgacc aggacgagct gctggcgatg
     2461 atgcgcgaga caggattcga gatcgaggat tcgttctacc agaaccgcgc cgcgatgctg
     2521 gcctggcgcc tgaattcgaa gatcttcaat cgcggcgcgc ttcccgccgc ccagtcgaag
     2581 atcttcgacc gcctcgtgcc gctgatcaaa gcgctcgacg gcgacaggcc gaagaagggg
     2641 ttgagtttga tagcgattgg gcggaagggt gtggggagtg gggggttggg ggtgggagca
     2701 aacgccgacg caacgcagcc ggtcgagacg ggtgtcgctg gtcgggggtc gggagtcgga
     2761 gcagaaacga caccatccac ttcagccgct tcgaacactg ctcaacttcc atgaacagcg
     2821 ccgctcctga ttcgtccgct aacgattcat ccggcgaaca tgcgtccgcg attgcttcaa
     2881 ctcccacacc ccacacccca ctccccacac cccgaaccac cctcatcctc gccggcggcg
     2941 ccggtacgcg cctgcgccca ctgtcatccg acgacaatcc gaagcagttt ctgcggatct
     3001 tcgacggcgt ctcgctgctc gagaagacgg tggcgcgcgt cgggccgctc gtttcgacgg
     3061 agtcgatctt catttcgacg aacgaccagt acgcggcgaa gtgcacggcg tttctgcccg
     3121 agctgccgaa aggcaacatc ctgacggagc cggcgcggcg aaacacggca ccggcaatcg
     3181 ctctttgctg ctttgcgatt gaagccatca caggcggtga tccggtgatc gccgtcctgg
     3241 cctcggatca ctacatcgcc gacgaggccg agttcgtacg cgtgctcgat cgcgcgtatc
     3301 agttcgcatc gaagaacgac tacctcgtgg ccatcgggat cgaaccgacg gagccgaaca
     3361 ccggctacgg ctacctgcag ctcggcgacg agatcgagcc ggggatcacc gcggtcgagc
     3421 gcttcacgga gaaaccgtcg cgcgagcgcg cggaggagtt tctggcggca ggcaactacg
     3481 cgtggaacgc cagcatgttc gtctggcggg ccagcgtctt ccggcgcgcg cttgcggccg
     3541 cggcgccgga gatcgcgaac gtcacgcgcg agaactacga gtcgatgccg tcgatctcga
     3601 tcgactatgc gctgatggag aaagcgccgc gcgttgccgc catccgcggc gacttcggct
     3661 ggagcgacgt cggctcgttc gaagcgctgg agcgggtggg agtgccggtg gcgtcgttgc
     3721 tggcgaaata caagtgaaca aaaaaaggca ttcaccacag agacacg
//
LOCUS       DATLVW010000143         4023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_252957, whole genome shotgun sequence.
ACCESSION   DATLVW010000143 DATLVW010000000
VERSION     DATLVW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252957"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VNN25_04680"
     CDS             complement(<1..639)
                     /locus_tag="VNN25_04680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXH90856.1"
                     /translation="MTPVLARSGWAGTFVVLSSLMLITTLTGCASVPAEPAPAPSPVS
                     RITLPSDEDDWSQLKAQMRVDLNRYNSREIARTPTGPTPTVTTQTRDREFAAKATAMF
                     APLSRMPLRLPVVGVRTSMLEDSWHAPRDGGNRVHKGIDIFAAKGTEVVAVVDGVISF
                     IGDQKLGGHCIWLTTENGASFYYAHLDRWAAGLYEGMEVQAGDLLGYVGNTGN"
     gene            complement(703..2037)
                     /gene="radA"
                     /locus_tag="VNN25_04685"
     CDS             complement(703..2037)
                     /gene="radA"
                     /locus_tag="VNN25_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXH90857.1"
                     /translation="MAKKQQAVFSCSECGGQSPKWLGRCPECNAWNSYGEEQIAPLIL
                     SSGTEPVAIGALDIDNAARTSTDIAALDRVLGGGLVAGAVTLVGGEPGIGKSTLLLQA
                     AEKLARDGVVLYVSGEESPRQIAIRARRLGATNNNIRLFAETSVERIVAVAESLKPVA
                     VIIDSIQTVHTITNASTPGSVGQVRDSAGRLMTAAKRLSIPFFLIGHITKEGTIAGPK
                     ALEHIVDTVLYFEGEKFQNYRVVRACKNRFGPANEVAIFEMHDSGLEEVANPSAALIS
                     QRSTAAGSAIVAAVEGTRPLLIEVQALVSATHFPSPRRMAIGMDANRVSLLLAVLEKK
                     NGGNFLASDVYANLAGGLQIDEPAIDLGVIAALISSQRNLPIAHDVVVFGEVGLLGEI
                     RSVSQADLRAREAAALGFRRIIIPQSNAAEIRADVEVLGVRRVDEFAAMLFG"
     gene            complement(2239..3309)
                     /locus_tag="VNN25_04690"
     CDS             complement(2239..3309)
                     /locus_tag="VNN25_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXH90858.1"
                     /translation="MKRFLCCTSVLLFAVTSFAADLPLPELFKRAKDEFTAGRYKESL
                     ADFELLDTNSARPGFENDRAKLIPVVTFYRGANLAALGRKDDAKAAFAMYLGLAPNAS
                     IASPPFPKATVDLFEQARKESRGRSTTMATSYASFAPPAGWTLPADEHWIESPVRYLL
                     TPAQKKEYATFTTNAERAAFVEAFWKQLDPTPDTAVNEFRAEVERRVAFADAMFGAGK
                     QAGRYNDRALIFTFLGPPTYAAMSRIGASEDALGSLRANGNDDMGRAARSSNSTNPAG
                     LSTLSGQQTDANLDQTLNRGTKESWIYRQGRIPQGIGFQEVRFQFLSKEGYGSGVLQK
                     DPDAMQTLGLAAEFARRDKKLN"
     gene            complement(3400..>4023)
                     /locus_tag="VNN25_04695"
     CDS             complement(3400..>4023)
                     /locus_tag="VNN25_04695"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF036996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HXH90859.1"
                     /translation="LIAANYDVPEVCLYFGGRLLRGCRAVKVSADGFAAFASPNIPPL
                     GTVGIDIEINWDLVRKPRPRQTMHVEEIAAPVVSALRLFPGISPELVRNVLRPPLQGL
                     VLETFGVGNGPDQNEAFLAALADATAHGVVIVDCTQCLEGTVDLTEYAAGTALARAGV
                     ISGFDMTAEAALAKLYYLFSRGYGAGKVKREVQRDLRGEMTVAKGQV"
BASE COUNT          728 a         1190 c         1346 g          759 t
ORIGIN      
        1 gttgccggtg ttgccgacgt agccgaggag atcgccggcc tggacttcca tcccttcgta
       61 aaggcccgcg gcccagcggt cgagatgtgc gtagtagaac gatgcgccgt tttcggtcgt
      121 cagccagatg caatggccgc cgagtttctg atcgccgatg aaactgatca cgccgtcgac
      181 cactgcgacg acttcggtgc ccttggccgc gaagatgtcg atgcccttgt gaacgcggtt
      241 cccgccatcc cgaggggcgt gccagctatc ctcgagcatt gaggttcgga cgccgacgac
      301 cggcaggcga agcggcatgc gcgaaagggg cgcgaacatc gcggttgcct tagccgcgaa
      361 ctcgcggtct cgcgtttgcg tcgtcaccgt tggcgtcgga ccggtcggcg tacgagcgat
      421 ctcgcggctg ttgtaccggt tgagatcgac gcgcatctgc gctttcaact ggctccagtc
      481 atcctcgtcg gacgggagag tgatcctgct gacggggctt ggagcaggcg caggctcggc
      541 gggcaccgac gcgcagccgg tgagagtagt gatgagcatg aggctgctga gcacgacgaa
      601 cgtgcctgcc cacccactgc gcgcgagaac cggagtcata ggagtagtgg tcttgggaac
      661 gacgggctga agtgtaaata attcgaagag ccaagtaaag catcatccga aaagcatcgc
      721 ggcgaactca tccacgcgac ggactcccag tacttcgacg tcggcgcgaa tctcggcggc
      781 gttcgactgc ggaatgatga tgcggcggaa gccgagggct gcggcttcgc gggcgcggag
      841 gtcggcttgg gagacactgc gaatctcgcc gagcaggccg acttcgccga agacgacgac
      901 gtcgtgcgcg atgggaagat tgcgctgcga ggagatgagc gcggcaatga cgccgaggtc
      961 gatggcaggc tcgtcgatct gcagaccgcc ggcgagattc gcgtaaacgt cgctggccag
     1021 gaagttgccg ccgttctttt tctcgagcac ggccagcaac agggagacgc ggttggcgtc
     1081 catgccgatc gccattctgc gcggggaggg gaagtgcgtc gcggagacga gcgcctgcac
     1141 ttcgatcaga agtggccgcg ttccttcgac ggcggcgacg atcgcggagc cggcggccgt
     1201 gcttcgttgc gagatcaatg ctgccgaggg attggccact tcctccagac cgctgtcgtg
     1261 catctcgaaa atcgcgactt cgttggcggg tccgaaacgg ttcttgcacg cgcgcacgac
     1321 acggtagttc tgaaacttct cgccttcgaa gtagagaacg gtgtcgacga tgtgctcgag
     1381 cgctttcggg ccggcgatcg tcccttcttt ggtgatgtgg ccgatgagga agaacggaat
     1441 ggagagacgc ttcgccgcgg tcatgaggcg gccggcggaa tcgcgcacct gcccgacgga
     1501 gcctggcgtg gacgcattcg tgatggtgtg tacggtctgg atcgaatcga tgatgacggc
     1561 gaccggcttc aacgactcgg ccacggccac gatgcgctcg actgaggttt cggcgaagag
     1621 gcggatgttg ttgttcgtcg caccgaggcg gcgcgcgcgg atggcaatct gccgaggcga
     1681 ctcctcgccg gagacgtaga gcaccacgcc gtcgcgtgcg agcttctccg cggcttgcag
     1741 cagaagcgtc gacttgccga tgccgggctc gccgccgacg agcgttaccg caccggcaac
     1801 gagcccgccg ccgagcacgc ggtcgagcgc ggcgatgtca gtcgacgtgc gagcggcgtt
     1861 gtcgatatcg agggcaccga tcgcgaccgg ctcggtgccc gagctcagga tcaacggcgc
     1921 gatctgttct tcgccgtacg agttccaggc gttgcactcg ggacagcggc caagccattt
     1981 gggcgactga ccaccgcatt cgctgcacga gaagactgcc tgctgtttct ttgccacgtg
     2041 aagcgattgt agcgaagcgg tgcgttcgtg ccgtcaactt cgccgattcc ctctcagcgg
     2101 cggtctccag cgttctacga aacgtagatc ggagagatca gagacaagag atcagagaca
     2161 agagattagg agatcggcgc cgggtttctg cttccttcca tcgatctcta atctctgatc
     2221 tcttgtctct gatctctttc aattgagttt cttgtcccgt cgtgcgaact ccgcggccag
     2281 gccgagggtc tgcatcgcat cgggatcctt ctgcagaacg cccgaaccgt aaccctcttt
     2341 cgacaggaac tgaaaacgca cttcctggaa cccgatccct tgcggaatcc ggccttgacg
     2401 atagatccac gattccttcg tgccgcggtt cagcgtctga tcgaggtttg cgtcagtctg
     2461 ctgcccggaa agcgtggaaa ggcccgcggg attcgtggag ttgctggagc gggcggcgcg
     2521 gcccatatcg tcgttgccgt tcgcacgcaa cgaaccgagg gcgtcttcgg atgcgccaat
     2581 cctggacatg gccgcgtagg tcggcgggcc aagaaacgtg aatatcaacg ctcgatcgtt
     2641 gtaccggccg gcctgtttgc cggcgccaaa catcgcgtcc gcgaatgcca cgcggcgctc
     2701 gacctcggca cggaattcgt tgacggcagt atccggtgtc ggatcgagct gcttccagaa
     2761 agcctcgaca aatgctgcgc gctcggcgtt cgtggtgaag gtcgcgtact ccttcttctg
     2821 tgccggggtg aggagataac ggacaggaga ctcgatccaa tgctcgtcgg cgggtagggt
     2881 ccagcccgcg ggcggcgcaa aggatgcgta ggacgttgcc atcgtcgtcg accggccgcg
     2941 tgactccttg cgggcctgct cgaagagatc gacggtcgct ttcgggaagg gcggcgaagc
     3001 gatcgacgcg ttcggcgcaa ggccgagata catggcgaag gcagctttgg cgtcgtcctt
     3061 gcggccgagg gcagcgaggt tcgcgccgcg atagaacgtg acgaccggaa tcaacttggc
     3121 gcggtcgttc tcgaagccag gccgcgcgct gttcgtatcg agcagctcga agtcggcgag
     3181 cgattctttg tagcgtcctg ccgtgaattc gtctttggct cgtttgaaaa gctcggggag
     3241 gggaaggtcc gcggcgaacg aagtcaccgc gaagagcagc acggatgtgc agcaaaggaa
     3301 acgtttcatg gtcagtgggc tcctgtgggt caggtctaaa gtctaaagtg ccgcagacga
     3361 ttttgcctcg ggcgacgttc gtcctcggca ctttagactt tagacctgac ccttggccac
     3421 cgtcatttcg ccgcgaagat cgcgctgtac ttcgcgcttc acctttccgg ctccatatcc
     3481 gcggctgaac aggtagtaca acttcgccag cgcggcctcg gcggtcatat cgaatccgct
     3541 gatcacgccg gcgcgggcga gagcggttcc ggcggcgtat tcggtgaggt cgacggttcc
     3601 ctcgagacac tgcgtgcagt cgacgatcac cacgccgtgg gccgtggcgt ccgcgagcgc
     3661 ggcgagaaac gcttcgttct gatcgggacc gttgccgacg ccgaacgtct cgagcacgag
     3721 cccctgcagc ggcggccgaa gcacgttccg cacgagctcg ggagagatac cggggaagag
     3781 tcgcagggcg ctgacgacgg gtgcggcgat ctcttcgacg tgcatcgtct gccgcggccg
     3841 cggcttgcgg acgagatccc agttgatctc gatgtcgatg ccaacggttc ccagcggtgg
     3901 gatgttcggc gaggcaaacg cggcaaatcc atccgcgctg actttcacgg ccctgcaacc
     3961 gcgcagcagg cgccccccga agtaaaggca aacctcgggg acgtcatagt tcgccgcgat
     4021 gag
//
LOCUS       DATLVW010000144         7170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_133240, whole genome shotgun sequence.
ACCESSION   DATLVW010000144 DATLVW010000000
VERSION     DATLVW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133240"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VNN25_04700"
     CDS             complement(<1..1636)
                     /locus_tag="VNN25_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH90860.1"
                     /translation="MNTAVRRLLIIACVVSAVSLSAQTKKKTTTMPSTPEPATVATDQ
                     AQPSADIPKGDEAATAPVTADTDKDASDPRATRLSLDGAVHTAIERNIGVQLQRYDLG
                     MAGAGILAAYSVFDPLASATLDHTNSQNPVSSAFQSSGGTGTDANASLRDTIPTGGNF
                     SITAANRRFTSTGNGTLFSPGYNASLGASFTQPLARNFGVDITRRGITIARNTLGINQ
                     EAFKVVLLDTTSAVEQAYYDLIYTRRFVDVVKEALFLARDQSRITQIRIDVGASAPLD
                     ILQPRVQIATGEENLINAVAAVRAAEDRLRALLNVAPEDWDRPIIPTDDIGYTPMTVN
                     IQDAVARAYELRPEIKEQQLTTEIRKVTYKYARNQVLPQFDLSLGYNAAGTAGTFNPP
                     VAGVSNTNFSHAAREVLNNDFPGWNVGVTIGVPVFNFGARAEKTRARLDMELSQAAEA
                     QSRQNIGVDVRTTARAIDTAAKNIFAAKTAREAAEENVNAERKRYENGMSTNFQVLQI
                     QQQLSDARGAELQALVGYNKAIAAYHRAIGDLLDTRN"
     gene            complement(1791..2840)
                     /locus_tag="VNN25_04705"
     CDS             complement(1791..2840)
                     /locus_tag="VNN25_04705"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXH90861.1"
                     /translation="MTVAPIEPPPTLPLGMGQPLLVVRDLTKYFPVKRGVLSRVVAQV
                     KAVDGISFGVSRGETLGLVGESGSGKTTVGRCILRLIEPTSGSVEFDGQDVLELDAGD
                     MRRMRRHMQIVFQDPYASLNPRMTVGTIIAEPLIIHGGMSSGDRASRVAQLLETVGLS
                     PDYQKRYPHEFSGGQRQRIGVARALATNPKFLVLDEPVSALDVSVQAQVVNLLQDLQE
                     QLGLTYLFIAHDLSVVEHISTRVAVMYLGKIMELAGRRELYADPLHPYTKALLSAVPI
                     PDPTRHKSRIILRGDIPTPLNPPSGCVFHTRCPIAQFPICSDVVPPLIEHKPGQFAAC
                     HFAGDPVAPAAILPS"
     gene            complement(3071..4135)
                     /locus_tag="VNN25_04710"
     CDS             complement(3071..4135)
                     /locus_tag="VNN25_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH90862.1"
                     /translation="MTQPIGLPGARVAYAEVDPREAILEVKNLRTSFPTGSGFVHAVD
                     NVSFNVRKGEALALVGESGCGKSVTAMSIMRLVAPPGRITAGEVHFKGRNLAELSERD
                     MRTVRGNDIAMVFQEPMTSLNPVFKIGAQVAEAIRIHNKVSRKQAWKRAGEMLELVSI
                     PDPIKRLDDYPHQLSGGMRQRVMIAMALSCDPELLIADEPTTALDVTIQAQIMELLAS
                     LQQRLGLAILLITHDLGIVAEFCERVIVMYTGRVVEESPVRELFASPAHPYTRGLLKS
                     LPSVSAGDPSAPHRLPTIKGMVPSISSLPRGCKFNPRCPDVMDICLGNEPALMLVSPG
                     HDARCYLHGDKADPERLEKS"
     gene            complement(4313..5980)
                     /gene="murJ"
                     /locus_tag="VNN25_04715"
     CDS             complement(4313..5980)
                     /gene="murJ"
                     /locus_tag="VNN25_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXH90863.1"
                     /translation="MSIDPTSTNVPPKPKPRAAGFAVLVAAGILLSRIAGLIRVRVFA
                     HYLGNSPAAGAYNAGLKIPNFLQNLFGEGVLSASFIPVYARLIAEDDDEMAGRVAGVI
                     LSLLGVIVMIIVTVCVFITPWIIDLIAPGFHGDVRQLTIRVVQILFPATGLLVFSAWC
                     LGVLNSHHKFFVSYVAPVVWNAAMIASLLFFGGRTSQSGLVIALSWGTVVGCALQFGI
                     QIPFVLKYAPRIKLSLQTALPPVRAVIRSFGPVVISRGVVQLSGYVDQIIASWLGTAA
                     VAGLSYAQTIYFLPISLFGMSVAAAELPGMSGAIGTDEEINAALRKRLERGFRQIAFF
                     VVPTVTAFIFIGRVLVAALYQSGAFHSDDTLYVWYILVGSTFGLLAVTLGRLYSSAFY
                     ALRDPKTPLRFAMLRVSLTAVLGLLFAFPLRPYIIDLIVLLHMPVPSLPEGPSLLGVI
                     GLTASAGMAGWIEFAMLRRALTRRIGSVKLAPSFLGRLWISGLSAALAGVAFHLYVSP
                     RITPHLPFRHISEAILVSGAFGLVYFGAAIALGVPEARATLQRFTRR"
     gene            6044..>7170
                     /locus_tag="VNN25_04720"
     CDS             6044..>7170
                     /locus_tag="VNN25_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXH90864.1"
                     /translation="MPNDRLIRIAGAGPSGLAAAIALARAGRNVEVHEAKRDVGTRFI
                     GDLQIIEGASESEPVPDFLDRLGIERNFYFRAANWATFYDHRNKGRVIRSDKPYGWFI
                     HRGAEEGTLDRGLLAQAQAAGAKVIFNSRLSPDQADIVATGPASPDGLAKEMTWRMGE
                     NVDRTLQSGNVGRTLQSDAAESRTGESDLRKPIPPELIDVYFNHKLAPGGYSYLFILD
                     GIATFGCAIVADFKRIDSYFDHSLAAAKRLHNLEIPADARTGYSYMNFHLKQQSTSNG
                     SRFVGEAAGFQDYLFGLGIRYALTSGSLAARSILEERDYDELWRHELGTKQETSLVNR
                     FLYESGGNFGLSMFVRQASRAKDFHRYLSGWHRSSWWKKMLS"
BASE COUNT         1380 a         2171 c         2346 g         1273 t
ORIGIN      
        1 tattgcgcgt gtcgagcagg tcgccgatgg cgcggtggta ggcggcgatg gccttgttgt
       61 aaccgaccag agcctgcaac tcggcaccgc gcgcatccga aagctgctgc tggatctgca
      121 gcacctggaa gttggtgctc atgccgttct cgtagcgctt gcgctccgcg ttgacgttct
      181 cctctgcagc ctcgcgagcc gtcttcgccg cgaagatgtt cttagcggcg gtgtcgatgg
      241 cacgggcggt cgtacggacg tcgacgccga tgttctgccg cgactgcgcc tcggcggcct
      301 gcgagagctc catatccagg cgggcccgcg tcttttctgc acgagcgccg aagttgaaca
      361 ccggcactcc gatcgtcaca ccgacattcc agccggggaa gtcgttgttg agaacctccc
      421 gggcggcatg cgagaagttg gtgttggaca ctcccgcaac gggcggatta aacgttccgg
      481 ccgttccggc cgcgttataa ccgaggctca ggtcgaactg cggaaggacc tgattgcgcg
      541 cgtacttgta cgtcaccttg cgaatctcag tggtgagctg ttgctccttg atctccggac
      601 gaagctcgta cgcgcgggcc acggcgtcct ggatgttgac ggtcatcggt gtgtagccga
      661 tgtcgtcggt cgggatgatg gggcgatccc aatcctcggg ggcgacgttg agcagagcgc
      721 gcagcctgtc ttcggcggca cgcaccgcgg ccaccgcgtt gatgaggttc tcttcgcccg
      781 tggcgatctg cacgcgcggc tggagaatgt cgagcggcgc cgacgcgccg acgtcgatgc
      841 ggatctgggt gatgcgggac tggtcgcgag cgaggaacag cgcttccttc accacgtcga
      901 cgaagcggcg ggtgtagatc agatcgtagt aggcctgttc gacggcggag gtagtgtcga
      961 ggagaacgac cttgaaggcc tcctgattga tgccgagggt gttgcgcgcg atcgtgattc
     1021 cgcggcgcgt gatgtcgaca ccaaagttgc gggccagcgg ctgcgtgaag gacgcgccga
     1081 ggcttgcatt gtagccggga ctgaagaggg taccgttgcc cgtgctcgta aagcggcggt
     1141 ttgcggcagt gatggaaaag ttgcctccgg tcgggatggt gtcgcggagc gacgcattcg
     1201 cgtctgttcc cgttccgccg ctggactgga aggcggaaga gacgggattc tgactgttcg
     1261 tgtgatcgag agtggcgctg gccagcggat cgaacacgct atacgctgcc agtatccccg
     1321 ccccggccat tcccaggtca tagcgctgga gctgtacgcc gatgttccgc tcgatcgccg
     1381 tgtgaacggc accatcgagc gagagacgcg tcgccctcgg gtcggaagcg tctttgtccg
     1441 tgtcggcggt aaccggcgcg gtcgcggcct catcgccctt gggaatgtca gcgctcggtt
     1501 gagcctgatc cgtagcaacg gtagccggct ccggcgtcga aggcatcgtc gtagtcttct
     1561 ttttcgtctg cgccgagagc gacacagcgc tcactacgca ggcaatgatc agcaaccgtc
     1621 gaacagcggt attcatgtaa accctcctgg acacgcctct tctacgcatc agtcaatgga
     1681 ttgtttcgct cgcgcgccgc ggcctgagcg atggccaagc ggaccgattt ccggcgggcg
     1741 cattgtattc gttgccggta cgtttgacgg aaagaaacgt ggaacgtcgc ttacgaaggc
     1801 aatatcgccg ccggcgcaac cgggtcgcct gcgaagtgac aggccgcgaa ttgacccggt
     1861 ttatgctcga tgagcggcgg tacgacgtcg ctgcagatcg gaaactgcgc gatcgggcaa
     1921 cgggtgtgaa agacgcatcc gctcggaggg ttgagcggcg tcggaatgtc gccgcggaga
     1981 atgatccggc tcttgtggcg agtgggatca ggaatcggaa cggcggacaa aagcgccttc
     2041 gtgtacggat gcagcggatc ggcgtaaagc tcgcgccggc ctgccagctc catgatcttg
     2101 ccgaggtaca tgacggctac gcgcgtggag atgtgctcaa ctaccgaaag gtcatgggcg
     2161 atgaacaggt aggtcaagcc gagctgctcc tgcaggtcct ggagcaggtt gaccacctgc
     2221 gcctggacag agacatcgag cgcggagacc ggctcatcga gaacgaggaa cttcggattg
     2281 gtggccaaag cacgggcaac gccgatccgc tgccgctgac cgcccgagaa ttcgtgggga
     2341 tagcgtttct gatagtcggg actgagcccg accgtttcga gaagttgcgc aacgcgcgat
     2401 gcacgatcgc cggatgacat ccctccatga atgatgagcg gctcggcgat gatcgtcccg
     2461 acggtcatgc gcggattgag cgatgcatac ggatcctgaa acacgatctg catgtggcgg
     2521 cgcatgcggc gcatgtcgcc ggcgtcgagc tcgaggacat cctgcccgtc gaattcgacg
     2581 ctaccggacg tcggctcgat cagcctgagg atgcagcgtc cgactgtggt cttgcccgat
     2641 cccgattcgc cgacgaggcc aagcgtctcc ccccggctga cgccgaagct gatgccatcg
     2701 accgccttca cctgcgcgac gacgcgcgag agaacgccgc gcttgacggg aaagtacttc
     2761 gtcagatcgc gcacgacgag aagaggttgg cccatgccga gtgggagggt gggcggcggt
     2821 tcgattgggg cgacggtcat gcgcgggctc cggggtttgc cgtcaaatgc cccctcatcc
     2881 ggccttcggc caccttctcc cccacaaaaa gcgtggggga gaaggcgctc gattggaggg
     2941 agtcgcgact tggatttcgc gcacccttca gcgactccct tccatcgagc gccttctccc
     3001 cccgggcttt ttcgggggga gaaggtgccg aaggcggatg aggggggcgt tgcaaaatac
     3061 gattagccat tcagcttttc tccaacctct ccggatccgc cttatcgcca tgcagatagc
     3121 aacgcgcgtc gtgacccgga ctgacgagca tcaacgccgg ctcgttgccg aggcagatgt
     3181 ccatcacgtc gggacaacga gggttgaact tgcagccccg tggaagactg gagatcgacg
     3241 gcaccatccc tttgatcgtc ggcagccgat gcggcgccga tggatcgccg gcgctgacgc
     3301 tcggcagcga cttgagcagg ccgcgggtgt acggatgcgc tgggctcgcg aagagctcgc
     3361 gaaccggcga ttcctcgacg acgcggccgg tgtacatcac gatcacgcgc tcgcagaatt
     3421 cggcgacgat gccgaggtcg tgggtgatga gaaggatggc cagcccgagg cgctgctgca
     3481 acgacgccag caactccatg atctgcgcct ggatggtcac gtcgagcgcg gtcgttggct
     3541 cgtcggcgat gagcaactcg ggatcgcacg acagcgccat cgcgatcatc acgcgttgcc
     3601 gcataccgcc ggaaagctgg tgcggatagt cgtcgagccg tttgatggga tcgggaatcg
     3661 atacgagctc cagcatctcc cccgcccgct tccacgcctg ctttcgcgac actttgttgt
     3721 gaatgcgaat ggcctcggcc acctgcgcac cgatcttgaa caccggattg agcgacgtca
     3781 tcggctcctg aaagaccatg gcgatgtcgt tgccgcgcac ggtgcgcatg tcccgctccg
     3841 aaagctcggc caggttgcgc cctttgaagt gaacctcgcc cgccgtgatc cgtcctggcg
     3901 gcgcgacgag gcgcatgatc gacatggccg tgacgctctt gccgcatccc gattcgccga
     3961 cgagcgccaa cgcctcccct ttccggacgt tgaaggagac gttgtcgacg gcgtgaacga
     4021 atccactccc tgtaggaaac gaggtgcgga gattcttgac ttcgaggatg gcctcgcgcg
     4081 gatcgacttc ggcgtaagcc acgcgcgcgc ccggcaaccc tattggctgc gtcatttgca
     4141 cgctccccgt catcggctgc tccgtcacgc aacgcggatc gtagcaagaa tggtgtcaga
     4201 tactggcgaa agttgacgag acccttgtca ccacagagac acagaggaca cagagttagg
     4261 cgtctctctc tgtgtcctct gtgtctccgt ggttcagcct ttttcgaccg aactaccgcc
     4321 gtgtgaaccg ttgcagcgtc gctcgcgcct cggggacgcc gagggcgatc gcggcgccga
     4381 agtagacgag tccgaaggca ccggagacca ggattgcttc gctgatgtgg cggaatggca
     4441 gatgtggcgt gatacgcgga gaaacgtaga gatgaaaggc cacgccggct agcgccgcgc
     4501 tcaatcctga gatccagagc cgcccgagga atgacggcgc gagcttgacg ctgccgatgc
     4561 gtcgcgtgag cgcgcgtcgc agcatcgcga actcgatcca gcctgccatt cccgccgacg
     4621 cggtgagccc gatcacgccg aggagcgatg ggccttccgg gagactcggc accggcatgt
     4681 gcagcaatac gatcaggtcg atgatgtacg ggcgcagcgg gaaggcgaag agcagtccca
     4741 gaacggcggt gagcgacacg cgcagcatgg caaagcggag cggcgttttc ggatcgcgca
     4801 gcgcgtagaa cgccgatgag tagagccggc cgagcgtcac ggcgagcagc ccgaaggtcg
     4861 agccgacgag gatgtaccag acgtagagcg tgtcatcgct gtggaacgcg cccgactggt
     4921 agagcgccgc cacgagcact cgtccgatga agatgaacgc ggtcacagtc ggcacgacga
     4981 agaacgcgat ctgccggaac ccgcgctcga ggcgcttgcg cagcgctgcg ttgatctcct
     5041 cgtccgtgcc gatggctccc gacattcccg gcaactccgc cgcggccacc gacatgccga
     5101 aaagactgat cgggagaaag tagatcgtct gcgcgtacga gagcccggcc acggcagcgg
     5161 tgccgagcca actggcgatg atctgatcga catagccgct cagttgcacg actccgcggc
     5221 tgatgaccac cggtccgaag ctgcggatga cggcccgcac cggcggcagc gcggtctgca
     5281 gagaaagctt gatccgcggc gcgtatttga gaacgaacgg aatttgaatt ccgaactgca
     5341 gcgcgcatcc gacgacggta ccccacgaaa gggcgatcac gaggccgctc tgcgacgtcc
     5401 gtccgccaaa gaaaaggagc gaggcgatca tcgccgcgtt ccagaccacc ggcgcgacgt
     5461 acgagacgaa gaacttgtga tggctgttca gaacaccgag gcaccacgcg gagaagacga
     5521 ggagtcctgt tgcaggaaag aggatctgga cgacacggat ggtcagttgg cgcacgtcgc
     5581 cgtgaaagcc cggcgcgatg agatcgatga tccatggcgt gatgaagacg cagaccgtga
     5641 caatgatcat gacgatcacg ccgaggagcg agaggatcac gcccgccacg cggccagcca
     5701 tctcatcgtc atcctcggcg atcagacgcg catacaccgg aatgaacgac gcggagagaa
     5761 ctccttcgcc gaagaggttc tgcaggaagt tcgggatctt gaggccggcg ttgtaggcac
     5821 cggcggctgg cgagttgccg aggtagtgcg cgaacacgcg gacgcgaatg agaccggcga
     5881 tgcggctgag gagaatcccg gcggcgacga gcaccgcgaa accggcggca cgcggcttcg
     5941 gtttcggcgg aacgttcgtg ctggtcggat cgatgctcat cgatgaagcg caagcctact
     6001 gcggaattcg cgccggaatc gcgcggtacc ataccgggaa gtgatgccga atgatcgtct
     6061 gatacggatt gctggtgccg gaccttcggg tcttgctgcc gccatcgcgc tcgcgcgtgc
     6121 gggacgcaat gtcgaggttc acgaggcaaa acgcgacgtc ggcacccgtt tcatcggcga
     6181 cctgcagatc atcgaaggag catcggagag cgagcctgtc cccgattttc tcgaccggct
     6241 ggggatcgag cgaaacttct acttccgggc cgccaattgg gccacgttct atgaccacag
     6301 gaacaaaggc cgcgtgatcc gcagcgacaa accctacggc tggttcatcc atcgcggcgc
     6361 cgaggaagga acgctcgacc gcggcctcct cgcgcaggcc caagccgcag gcgcgaaggt
     6421 catcttcaac agccgcctct cacccgatca agccgacatc gtcgccaccg gtccggcctc
     6481 gccggatgga ttggcgaagg agatgacgtg gaggatgggg gaaaacgtag atcggactct
     6541 ccagtccgga aacgtaggtc ggactctcca gtccgacgcg gcggagagtc ggactggaga
     6601 gtccgaccta cgtaaaccta tcccccccga gctcatcgac gtctacttca accacaagct
     6661 cgcccccggc ggctactcgt acctattcat cctcgacggc attgccacgt tcggatgcgc
     6721 gatcgtcgct gacttcaagc ggatcgattc ctacttcgat cactcgctcg cggccgcgaa
     6781 gaggctgcac aatctcgaga ttcctgccga tgcgcgcacc ggctactcgt acatgaactt
     6841 tcacctgaag cagcaatcga cgtcgaacgg ctcgcgcttc gtcggcgagg ccgcgggatt
     6901 tcaggactat ctcttcggcc tcggcatccg ctacgcgctc acgagcggct cgctcgccgc
     6961 gcgcagcatc ctcgaggagc gcgactacga tgagctttgg cgccacgagc tcggaaccaa
     7021 gcaggagacc agtctagtca atcgattcct atacgaaagc ggcggcaact tcggcctgtc
     7081 gatgttcgtt cgccaggcgt cgcgcgcgaa agactttcat cgctacctga gcggatggca
     7141 tcgcagcagt tggtggaaga agatgctctc
//
LOCUS       DATLVW010000145         5779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_295067, whole genome shotgun sequence.
ACCESSION   DATLVW010000145 DATLVW010000000
VERSION     DATLVW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295067"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..641
                     /gene="orn"
                     /locus_tag="VNN25_04725"
     CDS             75..641
                     /gene="orn"
                     /locus_tag="VNN25_04725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HXH90865.1"
                     /translation="MAPTRKPKRSKKHLVWIDCEMTGLDPTKDVLLEIATIITNYDLE
                     IIARGPVLAIRRTEAVLAAMDAWNRRTHKKSGLIRRVLTEGVSTAEAERQTLAFVKKY
                     CYVRTAPLCGNSIGQDKRFIAKYMPKLDAFLHYRTVDVSTIKVLVNEWYGDKYKCPKK
                     QEVHRALADIEESIAELAHYRKKVFVKS"
     gene            complement(648..1016)
                     /gene="rbfA"
                     /locus_tag="VNN25_04730"
     CDS             complement(648..1016)
                     /gene="rbfA"
                     /locus_tag="VNN25_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXH90866.1"
                     /translation="MRKTRRTSRVGESVRDALVEVFRFELTDLPLGLISITGVEVSPD
                     LHFARVFLSGLNEKETKDMVAELHQRRGFIRSQLGKRIHLRSTPELDFKFDETAMRAG
                     RIEDLLSDINKEPKSDDEES"
     gene            complement(1037..2011)
                     /locus_tag="VNN25_04735"
     CDS             complement(1037..2011)
                     /locus_tag="VNN25_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90867.1"
                     /translation="MNDDYLWDGSNEPGDADTDVRQLEALLARYRSAAPMPDFRRVVV
                     MRRRRWPLAVAAAAAIIAIVLGLAWFLRPRTSEWKATAIAGTSSLPDRPLRVGDVLHT
                     DSASRIRLESRSVGVIDVDGKSTIRLIESRKGRQRLALDVGVIHARTMSPPGVFVVDT
                     PQARAIDLGCEYVLAIAQGGSGELRVTSGWVDLSDGLWQSLVPQGASASISAGGRISV
                     PVFDDADPRFRAAVQNFEHAHDLNTILALSRKHDAFTLLNLFRLATPDEATRLYDRLN
                     ELVPAPPPMRREAVRNWQPSTTELWWRPVLAASGLGEIRKKKGMLDGL"
     gene            complement(2008..2526)
                     /locus_tag="VNN25_04740"
     CDS             complement(2008..2526)
                     /locus_tag="VNN25_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH90868.1"
                     /translation="MSARTDAELVLAAQAGDVASFEDLYVKYAPVVHSILLGRLPAAD
                     ADDVTQNVFMSALTKLNSLREPAAFAGWIARTARNAAEDHRRKFSETIEIDDKYATRA
                     TQGDDAEAAHALNLIRALPEAYRETLMLRLVEGMSGSEIAARTGLTPGSVRVNLHRGM
                     QLLRDALGGSAS"
     gene            2619..3179
                     /locus_tag="VNN25_04745"
     CDS             2619..3179
                     /locus_tag="VNN25_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90869.1"
                     /translation="MTRIIIPLHLLAILCASLLVGCTRAEAKSGLTSSVRIAGPEEPG
                     ERLVVTGHVLGRDGTPRGGVVIEVHHTDAHGVYLAEGAHGPNPAVAARLWGRLVTAPD
                     GSYRIETIKPGGYPGGGAPAHMHIHLRDGKSDEWALLEFAGDPNLTPAMIAKDGAGGT
                     FATIRPLERNATGVLHCTRDFRVGTK"
     gene            3176..3736
                     /locus_tag="VNN25_04750"
     CDS             3176..3736
                     /locus_tag="VNN25_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXH90870.1"
                     /translation="MRRAALFFLFALPLCAEPTRYYVKPVYTSLRFSIVKWGVLKEEG
                     IFRDFNGTLVYDPAHPEASRIDVVAKAASLDTKNDTRDQTVTGEDFLNAARYPTIEFH
                     STGVDHNFVTGNLTIHGTTRRIRFPVTSLGTHEIPSIGRMAGFETTFTINRRDYGVLG
                     ARWGAVPGVLSDAVELHITIGGITRP"
     gene            3836..4333
                     /locus_tag="VNN25_04755"
     CDS             3836..4333
                     /locus_tag="VNN25_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90871.1"
                     /translation="MTRFERWTLSLSALSTMVTIAGIVFVGLQLRGANDSLRTGVYSN
                     MSNWTFELDKAFLDHPELRPYFYDGKDIQQSDPLYFRAETMAEFILDNFDSIMKFDRT
                     YPETLHEGWRNWIYDSFSRSPLLVRTMEKLKPEYEPGPAWPIYADWKRRHSSQSPPKR
                     VTPLN"
     gene            complement(4362..4667)
                     /locus_tag="VNN25_04760"
     CDS             complement(4362..4667)
                     /locus_tag="VNN25_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXH90872.1"
                     /translation="MFVAVALFELHIEYAQSLKEKRMVVRSLRAKLRDRFEISAAEVA
                     LQDVHQRARMAISFVTGERTVAEGLLEKIQTFIESNTDAVLGGWTSELLDFDEETGL"
     gene            complement(4707..5693)
                     /gene="infB"
                     /locus_tag="VNN25_04765"
     CDS             complement(4707..5693)
                     /gene="infB"
                     /locus_tag="VNN25_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXH90873.1"
                     /translation="MGAKGVVHEAKKETGRGIVATVLIQDGTLKIGDAFFSGATSGRV
                     RAMTDERGKRINEAAPATPVQLTGFDDVPNAGDSLQVVDEQTQARMIGQMRQEKAREL
                     SQGKTSRMSLDQLFARMQSGTAKELNLILKADVQGSVEVLSDTMRKMSTAEVKVNVIH
                     ASVGAISTNDVLLASASDAIVVGFNVRPERSAGELAEKEGIDVRLYTVIYNLVDEIRL
                     AMTGLLDPKFQEVFQGRAEVRSTFKVPKAGIIAGCMVTEGLIPRAASVRLLRDNRVIL
                     EGKISSLRHFKNDVSEVRQGFECGIGIEKFQDIKIGDIIEAFKVEQLAAALA"
BASE COUNT         1103 a         1763 c         1820 g         1093 t
ORIGIN      
        1 ctgccgcatc cacgctgagc ggctccggta ttcttccccg gttcgcagcg aggaagcatc
       61 gaaccggagg ccacatggcg ccaacacgca aaccgaaacg cagcaagaag catctcgtct
      121 ggatcgattg cgagatgacc gggctcgatc cgacaaagga tgttctgctc gagattgcca
      181 ccatcatcac gaactacgat ctggagatca tcgctcgcgg gcccgtactg gcgattcgac
      241 gaacggaggc ggtgctggcg gcgatggatg cctggaaccg gcggacgcac aagaagagtg
      301 gattgatcag acgggtgctc accgaaggcg tcagcacggc cgaggcggag cggcagacgc
      361 tggcgttcgt gaagaagtac tgctacgtgc ggacggcgcc gctgtgcggg aactcgatcg
      421 ggcaggacaa gcggttcatc gccaagtaca tgccgaagct cgacgcgttt ctgcactacc
      481 gcacggtcga cgtcagcacg atcaaggttc tggtgaacga gtggtacggc gacaagtaca
      541 agtgcccaaa gaagcaggaa gtgcatcgcg cactcgccga catcgaggag tcgatcgccg
      601 agctggcgca ctaccggaag aaggtcttcg tcaagtcgtg agcggactta cgattcctcg
      661 tcgtcgctct tcggctcttt gttgatgtcg ctgagcaggt cttcgatgcg tcccgcgcgc
      721 atggctgtct cgtcaaactt gaagtcgagc tcgggggtcg agcggaggtg gatccgtttt
      781 ccgagttgcg aacggatgaa gccgcgccgc tgatggagct cggccaccat gtccttcgtt
      841 tccttttcgt ttagaccgct gaggaagacg cgggcgaagt ggagatcggg gctgacttcg
      901 acgccggtga tggagatgag tccgagcgga agatcggtca gctcgaatcg gaagacttcg
      961 acgagcgcgt cacgaacgga ctcgccgacg cgggaggttc tgcgggtttt gcgcatcagg
     1021 cggcgaggat aacaactcag agcccgtcga gcatgccttt cttcttcctg atctcgccga
     1081 ggccgctggc ggccaggacg ggacgccacc atagctcggt ggtcgacggc tgccaattgc
     1141 ggactgcctc acgccgcatc ggtggtggcg cgggcacgag ctcgttcagg cgatcgtaga
     1201 ggcgcgtggc ttcgtcggga gtggcgaggc ggaagagatt cagcagcgtg aaggcgtcgt
     1261 gcttgcggga gagcgcgagg atcgtgttca gatcgtgtgc atgctcgaag ttctgcacgg
     1321 cggcgcgaaa gcgcggatcg gcatcgtcga agacagggac gctgatccgg ccgcccgcgc
     1381 tgattgaggc cgaggcgccc tgcggaacca gcgattgcca gaggccgtcg ctcagatcga
     1441 cccagccaga ggtcacgcgc aactcgccgc tgcctccttg ggcgatggcg aggacatatt
     1501 cgcatccgag gtcgatggcg cgggcttgcg gggtatcgac gacgaacacc ccaggcggcg
     1561 acatcgtcct cgcgtggatc accccgacgt cgagcgcgag gcgttggcgg cctttgcggc
     1621 tctcgatcag gcgaatggtc gacttgccat cgacgtcgat gacgccgacg cttcgtgact
     1681 ccaggcgaat gcgtgacgcg gagtcggtat gcagaacatc accgacacgc aacggacgat
     1741 ctggcagcga ggacgtgccg gcgatggccg tggctttcca ttcgctggtt cgtggccgga
     1801 ggaaccacgc caaaccgagg actatggcga tgattgcggc ggcggccgca actgcgagcg
     1861 gccaacgtcg cctgcgcatg accacaacgc ggcgaaagtc gggcatcggc gctgcgcttc
     1921 gataacgggc gagcagtgct tcgagctggc ggacgtcagt atccgcgtct ccgggctcat
     1981 tgctgccgtc ccacaggtag tcgtcgttca tgaggcactc ccgccgagcg catcgcggag
     2041 aagttgcatt ccgcgatgca gattcacgcg caccgatccc ggcgtcaatc cggtgcgtgc
     2101 ggcgatctcg gaacccgaca ttccttcgac gagccggagc atcagcgtct cgcgatacgc
     2161 ctccggaagc gcgcggatca agttcagcgc atgcgccgcc tcggcgtcat cgccctgcgt
     2221 cgctcgtgtc gcgtacttat cgtcaatctc gatcgtctca gaaaacttgc ggcgatgatc
     2281 ctccgcggcg ttacgcgccg ttcgtgcgat ccagcccgcg aaggcggcag gctcacgaag
     2341 tgaattgagt ttcgtgagcg cggacatgaa cacgttctgc gttacgtcgt cggcgtcggc
     2401 ggcgggcagc cgcccgagaa gaatcgagtg cacgacgggc gcatatttca cgtagagatc
     2461 ctcgaaggac gcaacgtcac ctgcctgtgc ggcgagaacg agctcggcgt cggtgcgggc
     2521 agacatcagc gcctgtagga cgcggccggc gcccggagtg ttaacacgtt aacgctcgtc
     2581 atcgtcgtgg cgtcctcccg ccaaaggaga gctcaatcat gacgcgaatc atcatcccac
     2641 tgcaccttct ggcgattctt tgtgcatcgc tgctcgtcgg ctgcacgcgt gccgaggcga
     2701 aatcgggact gacttcgagc gtgcggatcg ccggtcccga agagcccgga gagcggctcg
     2761 tcgtcaccgg tcacgtcctc ggacgcgatg gcacaccccg cggaggcgtg gtcatcgaag
     2821 tgcatcacac cgacgcgcac ggcgtctatc tggccgaggg ggcgcatgga ccgaatccgg
     2881 cagtggcggc gcggctatgg ggccggctcg tgaccgcgcc ggacggcagc tatcgcatcg
     2941 aaacgatcaa gccgggcgga tatccgggtg gcggcgcgcc ggcgcacatg cacatccacc
     3001 ttcgcgacgg aaagagcgat gaatgggcgc tgctcgagtt cgcaggcgat ccgaatctga
     3061 ccccggccat gatcgcgaaa gacggcgccg gcggaacctt cgccacgatc cgtccgctcg
     3121 aacgcaatgc aacgggcgtc ttgcactgca cgcgcgactt tcgggtaggt acaaaatgag
     3181 gcgcgccgca ctgtttttcc tcttcgcgct gccgctctgc gcggagccga cgcgctacta
     3241 cgtcaagccg gtctacacga gcctgcgctt ctcgatcgtg aagtggggcg tgctgaagga
     3301 agaaggcatc ttccgcgatt tcaacggcac actcgtctac gatccggcgc atccagaggc
     3361 gtcgcgcatc gacgtcgtgg ccaaggctgc cagtctcgat acgaagaatg acacccgcga
     3421 tcagaccgtg accggcgagg acttcctcaa cgccgcgcgc tatccaacga ttgagtttca
     3481 cagcacgggc gtcgatcaca acttcgtcac cggcaatctg acgatccacg gcaccacgcg
     3541 ccgcatccgc tttccagtca cgtccctcgg cacgcacgag atcccgagca tcggcaggat
     3601 ggcaggattc gaaacgacct tcacgatcaa tcgccgcgac tacggagtgc tcggcgcgcg
     3661 gtggggtgcg gttccgggtg tgctcagtga tgcggtggag ctgcacatca cgatcggggg
     3721 aattacgagg ccgtaggaca ttgccaatcg ccattagttt caggagttat tgacatcgag
     3781 gagttggcga tttacagtca ggcacgtgtc gtttgaacac aaaccgggta atccaatgac
     3841 tcgttttgag cgttggacac tgtccctgtc cgctctttcg acgatggtta cgattgccgg
     3901 tattgtattc gttgggcttc agcttcgtgg tgccaatgac tctctgcgca ctggcgtgta
     3961 ctcaaacatg tccaattgga ccttcgaatt ggacaaggct ttcctcgatc atcctgagct
     4021 tagaccttat ttctacgatg gcaaggacat ccaacagagt gatccgctgt atttcagggc
     4081 tgagacaatg gcagagttta ttctcgataa ctttgattcc atcatgaagt tcgaccgcac
     4141 atatccagaa accctccatg aggggtggag gaactggatt tacgactcct tcagtcgcag
     4201 cccactcctt gtacgaacga tggagaagct gaaacccgag tatgagcctg gccccgcatg
     4261 gcccatttat gccgactgga agcgcaggca ttcttcacag agcccgccca aacgagtaac
     4321 tcccctcaac taagttccga gcagaacgag gctatctttc cctatagtcc cgtttcttcg
     4381 tcgaaatcga gcaactcgct cgtccagccg ccgagcaccg cgtcggtgtt tgattcgatg
     4441 aaggtctgaa tcttctccag cagtccctcg gcgaccgttc gctcgcccgt gacaaacgag
     4501 atggccatgc gagctcgttg gtgaacatcc tgaagcgcca cctcggccgc ggagatctcg
     4561 aaccggtcgc gcagtttcgc gcggaggctg cgcaccacca ttcgcttttc cttcagcgac
     4621 tgggcgtact cgatgtgcaa ttcgaagagt gcgacagcga cgaacatgcg aatgggagcg
     4681 cggcgggttg gccgccgcgc tccggattac gccaacgcag cagcgagctg ctcgaccttg
     4741 aatgcttcga tgatgtcgcc aatcttgatg tcctggaact tctcgattcc gatgccgcac
     4801 tcgaagccct ggcggacctc ggagacatcg ttcttgaagt gtcgcaggga gctgatcttc
     4861 ccttcgagga tcacgcggtt atcgcgcagc aggcgcacgc tcgcggccct cgggatgagt
     4921 ccctcggtga ccatacagcc ggcgatgatg cctgccttcg gaaccttgaa cgtggagcgc
     4981 acttccgcgc ggccctggaa gacttcctgg aacttcggat cgagcaggcc cgtcatggcc
     5041 agccggatct catcgaccag gttgtagatg acggtgtaga gacggacgtc gatgccttcc
     5101 ttctcggcca gctcgcccgc cgaccgctcg ggccggacgt tgaagccgac gacgatggca
     5161 tcggaggcgg atgcgaggag aacgtcgttt gtcgagatcg cgccgaccga cgcgtggatc
     5221 acgttgacct tcacctcggc ggtggacatc ttgcgcatcg tgtcgctcag cacttcgacc
     5281 gagccctgca catcggcttt gaggatgagg ttgagctctt tggctgtgcc cgactgcatg
     5341 cgcgcgaaaa gctgatcgag cgacatgcgc gaggtcttgc cctgcgaaag ctcgcgcgcc
     5401 ttttcctggc gcatctggcc gatcatgcga gcctgggtct gctcgtcgac gacctgcaaa
     5461 gaatcgccgg cgttcgggac atcgtcgaat ccggtcaact gcaccggcgt ggccggagcg
     5521 gcctcgttga ttcgcttgcc gcgctcgtcg gtcatcgcgc ggacgcggcc ggacgttgcg
     5581 ccggagaaga acgcatcgcc gatcttcagc gtgccgtcct ggatcagaac cgtggccacg
     5641 atcccgcggc cggtttcctt cttcgcctcg tgcacgacgc ccttggcgcc catctccggc
     5701 gaagccggcg gcatcaccca gcacatcggc gcgtatcagg tgcgccacaa cggcaagacg
     5761 atcaccttcc tggacaccc
//
LOCUS       DATLVW010000146         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_295153, whole genome shotgun sequence.
ACCESSION   DATLVW010000146 DATLVW010000000
VERSION     DATLVW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295153"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VNN25_04770"
     CDS             <1..743
                     /locus_tag="VNN25_04770"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90874.1"
                     /translation="SPVTSGDSSQPAFLSIVSDTFREQMEAIMTLGPRRLLSHRAVFV
                     ALIGTLSLATLGLGVAHAVPSGGGPTSKYKVGVTLTRESTPDVFNFAVTVCDRDSGAV
                     VSTANLRSSVEQWSMATSETTSKKSGHHTAVIRAIGHADGTAEVETTVDTEPAIIAKI
                     VAKRVDSQENLSDNPISLDLKDADIRDVLKTFAHLTNTEIVADDDVHGTVTLTLHNTP
                     WKEGLEKILADASLRSERSGNTIHVHRR"
     gene            773..1282
                     /gene="xrtH"
                     /locus_tag="VNN25_04775"
     CDS             773..1282
                     /gene="xrtH"
                     /locus_tag="VNN25_04775"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HXH90875.1"
                     /translation="MALAPRQTRFLIVFAILLIAFYALITIGWVDTHAVLPFTGALAR
                     VSGAALNLIGERVHVDGTIIAGPRFAVDIRGGCNGLEAVVFVCAAMLAFHAPMRKRLI
                     GAAAAAVILEALNIIRIDSLYLLGVYHRSIFETFHLAVWQTLMFGAAVLLFLIWTSKV
                     TPRDAPASL"
     gene            1263..1787
                     /locus_tag="VNN25_04780"
     CDS             1263..1787
                     /locus_tag="VNN25_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90876.1"
                     /translation="MLLRVSRFLAGLVLGVLLWWAATPAYNYVLAPATQLLLHADPRL
                     APLVLVPQERKIFVTSSTPIPTATIPADQLTYNIILLLALFASNERAWSLANLRGLAI
                     ALAILFASHCASLLISIESTYALRQGAWSEAHYGDAGANFWLTAELVFRIVGMFGVVF
                     AAWWGLSHSKSVST"
     gene            1811..2223
                     /locus_tag="VNN25_04785"
                     /pseudo
     CDS             1811..2223
                     /locus_tag="VNN25_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
BASE COUNT          392 a          736 c          664 g          431 t
ORIGIN      
        1 catcgccagt gacttccggt gacagcagcc agcccgcgtt cttgagcatc gtcagcgaca
       61 ccttccgcga acaaatggag gccatcatga ctcttggacc acgccgtctt ctcagccacc
      121 gtgccgtctt tgtcgctctc atcggaactc tttcgctcgc gacactcggg ctcggagtgg
      181 cgcacgccgt tccctccggc ggtggtccaa cttcgaaata caaggtcggc gtgacgttga
      241 cgcgtgagtc gactcccgat gtgttcaact tcgccgtaac cgtgtgcgat cgggacagcg
      301 gcgcagtggt ctcgacagcc aaccttcgca gcagcgtcga acagtggtcg atggcgacgt
      361 ccgaaacgac ttcgaagaag tccggacacc acacggcggt catccgcgcg atcggccatg
      421 cggatggcac agccgaagtt gaaacgacgg tagacaccga gccggccatc attgcgaaga
      481 tcgtcgcgaa gcgcgtcgac tctcaggaaa atctgtccga caaccccatc tctctcgatc
      541 tcaaagatgc cgacatccgc gacgtcctga aaacgttcgc gcatttgacg aataccgaga
      601 tcgttgccga cgacgacgtg catgggaccg tgaccctcac gctgcacaac acaccctgga
      661 aggaagggct cgagaagatc ctcgccgacg ccagccttcg ttcggagcgc agcgggaaca
      721 cgatccacgt gcatcgccgg tgaggtccgg cgccaactgc tagaatcgcc gcatggctct
      781 tgctccgcgg caaacgcggt ttctcatcgt ctttgcaatt cttctcatcg cgttctacgc
      841 gctcatcacc atcggatggg tggacacgca tgccgtgttg ccgttcaccg gcgcgctggc
      901 ccgcgtctcg ggcgcggcgc tgaacctgat cggcgagcgc gtgcatgtcg acggcacgat
      961 catcgccggg ccgagatttg cggtggacat ccgcggtggc tgcaacggtc tcgaggcggt
     1021 cgtcttcgtc tgcgcggcga tgctcgcctt tcacgctccg atgcggaaac ggttgattgg
     1081 cgctgccgcc gcggcggtca tcctcgaagc gctcaacatc atccgcatcg attcgctcta
     1141 cctgctcggt gtctatcacc gcagcatctt cgagaccttt cacctcgccg tctggcagac
     1201 gctgatgttc ggcgccgcgg tgctgctctt cctgatctgg acctcgaagg tcaccccgcg
     1261 cgatgctcct gcgagtctct aggtttctcg ccggcctcgt gctcggcgtg ttgctttggt
     1321 gggccgctac acccgcctac aactacgtgc tggcgccggc cacgcaactg ctgcttcacg
     1381 ccgatcctcg gctggcgccg ctggtgcttg tgccgcagga gcgcaaaatc ttcgtcacgt
     1441 catcgactcc gatcccgacg gcgacgattc ctgccgatca gctcacctac aacatcattc
     1501 tgctgctggc gctcttcgca tccaacgaac gcgcctggag tctcgccaat cttcgcgggc
     1561 tggcgatcgc gctggcgatc ctcttcgcgt cacactgcgc gagcctgttg atctcgatcg
     1621 aatcgaccta cgccctccgg caaggtgcgt ggagcgaggc ccactacggc gacgccggcg
     1681 cgaacttctg gctcaccgcg gagctggtct tccgcatcgt cgggatgttc ggtgtggtct
     1741 tcgccgcgtg gtggggactg agtcactcga agagcgtatc gacataagct gccggatcga
     1801 acggaatcag atcctcggcg ctgccgacac ctttcgcgca gccgcaatcg aacaactaca
     1861 gatgtgggcc ggccgcatcg gcgtcgatat ggtcaagcac caggcgggct ccgatccggg
     1921 ggcggttgcg cacgacaccg tcaccgcggc gaaggcgcgt ggcaccgacg tcgtcctcat
     1981 cgacaccgcg ggccgtcttc acaacaagtc gcacctgatg caggaattgt ccaaggttcg
     2041 ccgcgtgatc gagaaggagc tgccgggtgc gccacacgaa acgctcctcg tgctcgacgg
     2101 caccaccggc cagaacggcg ttagtcaggc ggcggcgttc ctcgaggctg cgcaggtcac
     2161 aggaatggtc gtgacgaagc tcgatggcac ggcgaaaggc ggcgtgattc tttcgatcat
     2221 gcg
//
LOCUS       DATLVW010000147         8669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_542865, whole genome shotgun sequence.
ACCESSION   DATLVW010000147 DATLVW010000000
VERSION     DATLVW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542865"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1715)
                     /locus_tag="VNN25_04790"
     CDS             complement(42..1715)
                     /locus_tag="VNN25_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH90877.1"
                     /translation="MKRAALTVLLFVVPFTAFAASAPTQAVIVMMKQTGRFTVKSLAA
                     NLNPSVSDGERDVRDLPGIHGFAANLTADEIASLKASGKVISIDPDLERHALTDSVSA
                     GQQTTPYGLTAIDAPPVWPVTRGKSLSNGPAIHVAIIDTGIDYNYSELAAAFKAGFNY
                     ITRTADPLDDNGHGTHVAGTIAAANDGAGVVGIASDVDIYSLKVLDTCGSGRTSDIIQ
                     ALQWVVDKKKEIGGNWIINLSLGSDTPSTPEQTAFDAASNAGILIFAAAGNSYDGTDG
                     LSYPAGYPSVVSVGAVDSNNLIASFSQRGADLKLVAPGVNVISTFLNGRVATDDGRKI
                     PANFADGTLSSGDSACLSKGPVTAKFVSGGTGNATEIPASVSGKIALIERGGTDPSGT
                     GLTFLAKTRNALAKGAIGVVVYSNANPVVTPGFSALTQTDVNTLPPMALIERADGLAL
                     LATPNVTLTMSFDSHGLTELFALLNGTSMATPHAVGAAALVWAASPNSTSTNVATALE
                     QTAKDLGTAGQDTTYGYGLVDALAAAKQLNPAAFVPAPVKGRQPGRRGH"
     gene            1841..2284
                     /locus_tag="VNN25_04795"
     CDS             1841..2284
                     /locus_tag="VNN25_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXH90878.1"
                     /translation="MTEWKTNLIDSNEGIGTLLRDTKTIAVLGIKPESHAGQAGHYVP
                     AHMAQAGYEIIPVPVYYPEVTEILGVPVVRTLAAIGRPIDMVNVFRKPSDIPAHVDDI
                     LAAKPKSVWFQLGIRNDEAAQKLAEAGIKVVQDRCLMVEEGRHRR"
     gene            2341..3798
                     /locus_tag="VNN25_04800"
     CDS             2341..3798
                     /locus_tag="VNN25_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH90879.1"
                     /translation="MPTHRRVDLPPFTFWETRAGHGTPIVLLHGLGGSSDWWRYNIDI
                     LAESHLVSAVDLVGFGRNRLFMRQSKLPLRFDDIAALLARWIESSFAEPVHLVGNSMG
                     GHIAIHLAASRPDLVRSLVLVDATGIPFEVAPGAHIESLAMPRGWRTFLLILARDLFR
                     AGPTAIALSFARLLRDDARPLLKTLTLPVLLVWGETDPLVPLTYARQILEILPNARLR
                     VISRAGHVPMWENPREFNEALLSFLDELERGSNVSEPAFSWGLSGWTNGIAHRAAGRR
                     RDVVLVHGLGMSSAYFIHFARALHARGTNPIAPDLAGFGESVDGPAAGPEEHARSLAA
                     WADALSIRDAVWIGHSVGCNAVAHLQRSRPDLVRRLICIGPLWSPRHPSRLLFSLLID
                     AFREPLPLFGYVLRAYLRCGIGRWIATFRRYAKDLRSAPPECLMLAGKRDPLVDRDAI
                     ASLTFVVGAHACHYSNPEEAADSCELDVAERTASS"
     gene            complement(3841..4329)
                     /locus_tag="VNN25_04805"
     CDS             complement(3841..4329)
                     /locus_tag="VNN25_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXH90880.1"
                     /translation="MLLPFDQVIEISVRLLAAVLIGGCIGLDREIRRKPAGMRTHALV
                     SLGAALVMLIVVRTGPGQIEHVDAVSRAIQGIIAGVGFLGGGAILKSEHDIIHGLTTA
                     ASIWLVASLGIACGVGQWVAALIALALALLVLIVGEPVERLVHRIANRETGNVTGNER
                     RD"
     gene            4446..4937
                     /locus_tag="VNN25_04810"
     CDS             4446..4937
                     /locus_tag="VNN25_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HXH90881.1"
                     /translation="MRSHKLFLLAVLTISIATLSAAPPPTGADAVVSQLYKDFAWETV
                     PDHAEKPGLIDQPRAVLRRYFDDGLTNLILEDRACAERTHEICRLDFSPIWDSEDPGA
                     SGLKVSATKDPSVVRVTFRYPGSNELMDLSYRMTKTKDGWRIHDIAYHSHASLLSLLR
                     AKM"
     gene            4976..5380
                     /locus_tag="VNN25_04815"
     CDS             4976..5380
                     /locus_tag="VNN25_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH90882.1"
                     /translation="MTTESLPFLGRTISASLTVKDLQKSLDWYQDVVGFTVDRKHERE
                     GKLVAVSLKAGEIRLLIGQDDGAKGWDRAKGEGFSLHITTDQNIDELANRIKARGGSL
                     LSEPADMPWGPRVFRVQDPDGFKITISSVPAA"
     gene            6035..6409
                     /locus_tag="VNN25_04820"
     CDS             6035..6409
                     /locus_tag="VNN25_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90883.1"
                     /translation="MNMPFEFGIEYGCRLFGAEPLTEKRCLVLEKDPHEFRKAISDLS
                     GIDIKHHGNEPEGIIRAVRDWFVETVGLRRVHSATRIWRRFNVFTSSFYVARLADGFT
                     DRDLNMMPIPEYIDFTEAWLAE"
     gene            complement(6429..6728)
                     /locus_tag="VNN25_04825"
     CDS             complement(6429..6728)
                     /locus_tag="VNN25_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH90884.1"
                     /translation="MRRLIVEPRAEREIAVASKWWHRNREKAPSAFDDDVAAAIDEVR
                     IEPGLGVSIAGTRRPNVRRVTLQRVRYYVYYRVNEQDALVVLAIRHTSRRPPRGL"
     gene            complement(6709..6873)
                     /locus_tag="VNN25_04830"
     CDS             complement(6709..6873)
                     /locus_tag="VNN25_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90885.1"
                     /translation="MKHQPAETDEVVELTPEEIAELDDRADAVERGEFVDGDEVLRRL
                     RSGYYATTDR"
     gene            complement(6870..>8669)
                     /locus_tag="VNN25_04835"
     CDS             complement(6870..>8669)
                     /locus_tag="VNN25_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HXH90886.1"
                     /translation="SRYETLSSEIGETTVRSGYDKEELIILDEFIEHGRAIRAERERA
                     ASVGEKPNFIPLVRSWGGVTIAYRKSIADAPAYRLNHEEVIKAFEEGIAYAESLSPVE
                     AIADEFGHVKSLLFEKQINEDGRWKDSGNIIELDARSVMVAAGTSPNVIYEKEHPGTF
                     KLDKYGQFFQNFAVSAADAGPELIEVDGNRELGFFTSYQHPASREKVISFYGDNHPRY
                     AGNVVKAMASAKDGYQHVARLFTRELMQLDRSAEAQVARDAQWSGLLQTLDDGLLAVV
                     AQINRLTPTIVEVVIRAPFASQHFEPGQFFRLQNFEANAPLIDGTRLTMEGIALTGAW
                     TDPERGLLSLIVLELGGSSRLCAQLKPGEPVVVMGPTGAPTEIPHNETVLLAGGGLGN
                     AVLFSIARAFKMNGCKVVYFAGYKKKDDVFKRDEIEAGTDQVIWSVDAGDPIEPRRPQ
                     DRGFTGNIVQSILAYAQTSPEFDLRNVQRLIAIGSDGMMRAVKDARHGVLAPFLNDHH
                     VGIGSINSPMQCMMKQVCAQCLQRHVDPITGKESFIFSCFNQDQPLDSVDFTNLRARL
                     RQNTVVEKMTNLWLTRLLAKQQAKPAEVSLEMS"
BASE COUNT         1551 a         2675 c         2721 g         1722 t
ORIGIN      
        1 gacgctcgaa ggcgccccgt cggggtttga aagcgaaaac gttagtgacc gcgccggccc
       61 ggctgacgcc ccttcacggg cgccggcacg aacgccgccg ggttgagctg ctttgccgcg
      121 gccagggcgt ctacgagacc atagccatag gttgtgtctt gtccagcagt accgaggtcc
      181 ttcgcggtct gttcgagtgc agtagcgaca ttggtgctcg tagagtttgg cgaggccgcc
      241 cagaccagag ccgccgcgcc aaccgcgtga ggcgtggcca tcgaagtgcc gttcaggagc
      301 gcaaagagct cggtgaggcc gtgactgtcg aagctcatag tcaatgtcac attcggggtg
      361 gccagcagtg ccagaccgtc cgcgcgctcg atcagcgcca ttggaggcag tgtgttcacg
      421 tccgtctgcg ttaaggcgct gaagccgggt gtgacgactg ggtttgcatt gctgtaaacc
      481 acgacgccga ttgcgccctt cgccagagcg ttgcgcgttt tcgccagaaa cgtgagacct
      541 gtgccgctcg gatcggtacc accgcgttcg atgagcgcga tctttccgct gacgctggcc
      601 gggatttccg tcgcgttgcc ggtgccgccg gacacaaact tggcggtgac cgggcctttg
      661 gagaggcatg cggagtcgcc gctactcagt gtgccgtcgg caaaattcgc cggaatcttc
      721 cggccatcat ccgtcgccac acggccgttg aggaacgtgg agatcacgtt aacgccgggc
      781 gcgacgagct tgagatcggc acctcgctgc gagaagctgg cgatgagatt gttggagtcg
      841 accgcgccta ccgaaacgac cgatgggtat ccagcgggat acgacagtcc gtcggtcccg
      901 tcgtagctgt ttccggcggc ggcaaagatg aggatgcctg cgttgcttgc ggcgtcgaac
      961 gcggtctgtt ccggcgtgga cggagtgtcg gatccgagcg agagattgat gatccagttt
     1021 ccgccgatct ctttcttctt atccacaacc cactggagcg cctggatgat gtcgctcgtc
     1081 cggccgctgc cgcaggtgtc gagcactttc agagaataga tgtcgacatc cgaggcgatg
     1141 ccgacgactc cggcaccatc attcgcggca gcgatggtcc cggcaacatg ggtgccgtgg
     1201 ccgttatcgt cgagagggtc agcggtacgg gtgatgtagt tgaagccggc cttgaaggcc
     1261 gcggcaagct cggagtagtt gtagtcgatg ccggtgtcga tgatggccac gtgaatggcc
     1321 ggaccgttcg acagtgactt gccgcgcgtg accggccaaa ccggcggtgc gtcgatggcc
     1381 gtcaatccgt aaggtgtggt ctgctggccc gcgctcacgg aatcggtgag ggcatggcgc
     1441 tcgaggtccg gatcgatcga gatgaccttg cccgatgcct tgagcgatgc gatctcgtcg
     1501 gcggtgaggt tggccgcgaa tccgtgaatg ccgggcaggt cgcgaacgtc gcgttcgccg
     1561 tccgagacac tcggattgag attcgccgca agagacttga cggtgaagcg gccggtttgc
     1621 ttcatcatca cgatgacggc ctgcgtcggc gccgaggcgg cgaacgcggt gaacggaaca
     1681 acgaagagaa gcacggtcag ggctgcacgt ttcatccgac gatagacctc ttgttatttg
     1741 gacttgtcgc gtaaaaagga gacgccgctg gaggttattc cgtgtaactc gatcgtatca
     1801 ccgcaccgcc gttgcgcgct ttttgctacc ctccgccacc atgacagagt ggaaaacgaa
     1861 cctcattgat agcaacgaag gcatcggcac gctgctgcgc gacacgaaaa cgatcgccgt
     1921 cctgggcatc aaaccggagt cgcacgcagg gcaggcaggc cattacgttc ccgcccacat
     1981 ggcccaggcg ggctacgaga tcatccccgt tcccgtttac taccccgaag tcaccgagat
     2041 cctcggcgtg ccggtcgttc gaacgctcgc cgcgatcggc cgtccgatcg acatggtcaa
     2101 cgtcttccgc aagccatccg acatccctgc gcacgtcgac gacatcctcg cggcaaagcc
     2161 gaagagcgtc tggttccaac tcggcatccg caacgatgaa gcggcgcaga agctggccga
     2221 ggcagggatc aaggtggtgc aggaccggtg tctgatggtg gaagagggac ggcatcgaag
     2281 gtaggtgttt ctccgcgttc tgcgcgcctc cgcgtgaaac ggttgtgaac ccgcattgac
     2341 atgcccacgc atcgccgcgt cgaccttccg ccgttcacct tctgggaaac gcgcgccggg
     2401 cacgggacgc ccatcgtcct tcttcacggc ctcggcgggt cgtccgattg gtggcgatac
     2461 aacatcgaca tcctcgccga gagtcacctg gtgtcggccg tcgatctcgt cggcttcgga
     2521 cgcaatcgcc tgttcatgcg gcagagcaag ctccctctgc gattcgatga cattgccgct
     2581 cttctggcgc gatggatcga atcgtcattc gccgagccgg tccacctcgt tggcaactcg
     2641 atgggcggac acatcgccat ccacctggcg gcgagccgtc ccgacctcgt acgttcgctg
     2701 gtactcgtcg acgctaccgg catcccgttc gaggtcgcgc ccggcgcgca catcgagagc
     2761 ctggccatgc cgcgtggctg gcgaacgttt ctgctgatcc tggcgcgaga tctctttcgt
     2821 gccggcccga ctgcgatcgc gctttcgttc gcacgcctgc tgcgcgatga cgcccggccg
     2881 ctgctgaaga cgctgacgct tcctgtgctt ctcgtctggg gggagacgga tccgctcgtg
     2941 ccgctgactt atgcgagaca gattctcgag atcctgccga acgccaggtt gcgcgtgatc
     3001 tcccgcgccg gccacgtgcc gatgtgggag aaccctcgcg aattcaacga ggcgctcctg
     3061 tcatttctgg acgagctcga acgcggctcg aacgtttccg agccggcgtt ttcgtggggc
     3121 ctgtcgggat ggacgaacgg aatcgcccat cgcgctgccg gccgccggcg cgacgtcgtg
     3181 ctcgttcacg gactcggcat gtcgagcgcg tacttcatcc acttcgcgcg ggcgctccac
     3241 gcgcgcggca cgaatccgat cgctcccgat ctagccggtt tcggcgagag cgtcgatggt
     3301 ccggctgccg gccccgagga gcacgcgcgc agcctggcag cctgggcgga cgcattgtcg
     3361 atccgcgatg cggtgtggat cggtcattcg gtcggatgca acgccgtcgc gcatctccaa
     3421 cgttcgcgac ccgacctcgt gcgccgcctt atctgcatcg gtcccctatg gtcgccgcgc
     3481 catccgtccc gactcctgtt ctcgctgctc atcgacgcgt tccgcgagcc gctgccgctc
     3541 ttcggatacg tcctccgcgc ttatctccgc tgcggcattg gacgctggat cgcgacgttt
     3601 cgccgctacg ccaaagatct ccgcagcgcg cctcccgagt gcctgatgct cgctggtaag
     3661 cgcgatccgc tcgtcgaccg cgacgccatc gcgagcctga cgttcgtcgt cggagcacac
     3721 gcctgtcatt actcgaatcc cgaggaggcg gcggatagtt gtgaactcga tgttgctgag
     3781 cgtactgctt cttcgtagcg ccggcggctg gtgcggcaat gtcaggcaga cgggtgacgc
     3841 ctagtcgcgc cgctcgttgc ccgtgacgtt tccggtttct cgattggcga tccgatgcac
     3901 gagccgttcg accggctcgc cgacgatgag gacgagaagt gccagcgcca gagcgatgag
     3961 cgccgccacc cattgcccga cgccgcaagc aatccccaga gaagccacga gccagatcga
     4021 tgccgccgtc gtgaggccgt ggatgatgtc atgctcgctc ttgagaatcg ctccgccgcc
     4081 gagaaatccg actccggcga tgatcccctg aatggcccgg ctgaccgcgt cgacatgctc
     4141 gatctgcccc ggtcccgttc gcacgacgat cagcatgacc agcgccgcgc cgagcgagac
     4201 gagcgcatgc gtccgcatcc ccgccggctt ccgccggatc tcgcgatcga gcccgatgca
     4261 cccgccgatc agcaccgcgg caagaaggcg gaccgagatc tcgatcacct ggtcgaatgg
     4321 gaggagcatg gccgaattgt ttcacaggag cgagccggag gtcgttggga ctgtgcatta
     4381 ccatgtggcc aagtccgcgg cattcgcagc ggccgaacat cagtcagtac gttaggaaga
     4441 atcagatgcg ctcgcacaaa ctgttcctgc tcgcagtcct cacgatttcg atcgccactc
     4501 tctccgccgc gccgcctcca accggcgccg acgccgtagt atcgcaactg tacaaagact
     4561 tcgcgtggga gacggtgccc gatcatgcgg agaagcccgg cctgatcgac cagccgcgcg
     4621 cggttcttcg ccgctatttc gacgacggcc tcacgaacct cattctcgaa gatcgcgctt
     4681 gcgccgaacg aacgcatgag atttgccggc tcgacttctc gccgatctgg gattcggagg
     4741 atcccggtgc gagcgggctg aaggtatccg cgacgaaaga cccgagtgtc gtgagagtga
     4801 catttcgtta cccggggagc aacgagctga tggatctgtc ctatcgcatg acgaaaacca
     4861 aagacggttg gcgcattcac gacatcgcct accactctca cgcctcgctg ctctcgctct
     4921 tgcgcgcgaa gatgtaagac cttcccaaca tccgccatcg aatccaggag acttcatgac
     4981 cacagagtcc cttcccttcc tcggccgcac catttccgcc tcgctcaccg tgaaggatct
     5041 tcagaagagc ctcgactggt atcaggatgt agtgggattt acagtcgatc ggaagcacga
     5101 acgcgaagga aagctggtgg cggtgtcgct gaaagcgggc gagatacgcc tcctgatcgg
     5161 tcaggacgat ggagccaaag ggtgggatcg agccaagggc gagggattct cgctgcacat
     5221 cacgaccgat cagaacatcg acgagctcgc gaaccggatc aaagcccgag gcggaagcct
     5281 tttgtcggag ccagccgata tgccgtgggg cccgcgggtg ttccgcgtgc aagacccgga
     5341 cggattcaag atcacgatct cttcggttcc cgcggcgtga cgtatcacga ccgcctctgc
     5401 gcggtcattg cgcacttcgc tggagatttc gggatgcgag acaggcgcga gcgttgatgg
     5461 agaagttggg gtgggaaggt gcgggttttc tggttcacgt gaccgcggtg cgggtaggcg
     5521 agtgttatca tttacaacga tggacaagct cgtgaactct ctccaggtgt ttcctacgat
     5581 gggcggagga tggagcgtcg cgaagtgggg cgcaatacgc gccttgaagc gattccccac
     5641 caaagaagca gccgaggcgt gggcccgcgc acgaagtatc aaggaacgca ccgttctttt
     5701 catccaccgg agagacggca ccgttgctgc gatgagatcg tacgggaacg gtcctcatcc
     5761 ggcgcgcgct cggaaacctt aagtggagca acaaccgcac gccttcgagc ggagcgtatt
     5821 cgtcaactgc cccttcgata gcgactatct gcccctcttg cgtcctttgc tcttcacaat
     5881 cctctatctg cggtttaggc cgcgtatcgc gtccgaacgc tccgactccg gggagagccg
     5941 gatcgataag atctgctcgt tgatccgcga gtgccgatac agcgtccacg atctctcccg
     6001 cctacgtgct ggcgaaatcg gagagatttc acgaatgaac atgccgttcg agttcggtat
     6061 cgagtatggc tgtcgtttgt tcggagcgga gcctctgacc gagaagcgtt gtctcgtgct
     6121 tgagaaggat ccccacgagt ttcgtaaggc gatctctgat ttgtccggca tcgatatcaa
     6181 gcatcacggc aacgagcctg agggaatcat cagagcggtt cgcgattggt ttgtagaaac
     6241 agtcggtctg cggcgcgtac acagtgcgac tcggatctgg cgacgattta atgtcttcac
     6301 ttcgtcgttc tacgttgcac gcctcgccga tggttttacg gaccgcgacc tgaacatgat
     6361 gccgattccg gaatacattg acttcaccga ggcgtggctc gccgagtagg tcgcatcgaa
     6421 tcctgttttc acaaaccgcg gggcgggcgg cgggaggtat gtcgaatcgc aagcacaacc
     6481 agcgcatctt gttcgttgac gcggtagtag acgtagtatc ggacgcgttg gagagtcacc
     6541 cggcggacgt taggacgtct tgtgccggca atggagacgc cgagcccggg ttcgatgcgg
     6601 acctcatcaa tcgcggcggc gacatcatca tcgaacgccg acggagcctt ttcccgattg
     6661 cggtgccacc atttcgaagc gaccgcaatt tcgcgctcag cacgaggctc aacgatcagt
     6721 cgtcgcatag tagccgctgc gaagacgccg aagcacctcg tctccgtcaa cgaactcgcc
     6781 acgctcgact gcgtctgcgc ggtcgtcaag ttcggctatc tcttccggcg tcagctcgac
     6841 tacttcatcc gtttctgcgg gttggtgctt catgacatct ctaatgatac ctcggcaggc
     6901 ttcgcctgtt gcttcgccag cagccgcgtc agccacagat tcgtcatctt ctccaccacc
     6961 gtgttctgcc gcaggcgtgc gcgcaggttc gtgaaatcga ccgagtcgag cggttggtcc
     7021 tgattgaagc aactgaagat gaaggactct ttgccggtga tcgggtcgac gtggcgttgc
     7081 aggcattgtg cgcagacctg tttcatcatg cattgcatcg gcgagttgat cgagccgatg
     7141 ccgacgtgat ggtcgttgag gaacggggcg aggacgccgt gccgcgcgtc tttcaccgcg
     7201 cgcatcatcc cgtcggagcc gatggcgatg aggcgttgga cgttgcggag gtcgaattcc
     7261 ggcgacgtct gcgcatacgc cagtatcgac tgcacgatgt tgccggtgaa gccgcggtcc
     7321 tgcgggcggc gcggctcgat cggatcgccg gcatcgaccg accagatcac ctgatcggtg
     7381 cccgcttcga tctcatcgcg tttgaagacg tcgtccttct tcttgtagcc ggcgaagtag
     7441 acgaccttgc agccgttcat cttgaacgcg cgggcgatcg agaagaggac ggcgttgccg
     7501 aggccgccgc ctgcgaggag aacggtctcg ttgtgcggga tttcggtcgg cgcgccggtt
     7561 ggccccatca ccacgaccgg ctcgcccggt ttgagctgcg cgcaaagacg tgacgagccg
     7621 ccgagctcga ggacgatcag cgagagcagg ccgcgctcgg ggtcggtcca cgcgccggtc
     7681 agcgcgatcc cttccatcgt caggcgggtg ccgtcgatca gcggcgcgtt cgcttcgaag
     7741 ttctgcagac ggaagaactg gccgggctcg aaatgctgcg atgcgaacgg tgcgcggatg
     7801 acgacttcca cgatcgtcgg cgtcaggcga ttgatctgcg ccacgacagc gagcagaccg
     7861 tcgtcgagcg tctgcagcag tccgctccat tgcgcatcgc gcgcaacctg cgcctcagct
     7921 gaacgatcga gctgcatcag ctcgcgtgtg aagaggcggg cgacgtgttg ataaccatcc
     7981 ttcgcggagg ccatcgcctt gacgacgttg ccggcgtagc gtgggtgatt gtcgccgtag
     8041 aacgagatca ccttctcgcg cgacgccgga tgctgatacg acgtgaagaa cccgagctcg
     8101 cggttgccat cgacttcgat cagctcgggg ccggcatcgg ccgccgagac cgcgaagttc
     8161 tggaaaaact gtccgtactt gtcgagcttg aatgtgcccg gatgttcctt ctcgtagatc
     8221 acgttcgggc tcgttcccgc ggccaccatc acgctgcgcg cgtcaagctc gatgatgttt
     8281 cctgagtctt tccagcggcc gtcttcgttg atctgcttct cgaagaggag cgacttcacg
     8341 tggccgaact cgtcggcgat cgcttccacc ggcgagaggc tctcggcgta agcgatccct
     8401 tcctcgaagg ccttgatcac ttcttcgtgg ttgagccgat acgccggcgc gtcggcgatc
     8461 gattttcggt aggcgatcgt cacgccgccc caggagcgaa cgagcgggat gaagttgggc
     8521 ttctcgccaa cggacgcagc ccgctcacgc tcggcgcgga tagcgcggcc gtgctcgata
     8581 aactcatcga ggatgatcag ttcctctttg tcgtagccgc tgcggactgt cgtctcgccg
     8641 atctcggagg agagcgtctc gtagcggct
//
LOCUS       DATLVW010000148         6658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_584176, whole genome shotgun sequence.
ACCESSION   DATLVW010000148 DATLVW010000000
VERSION     DATLVW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584176"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VNN25_04840"
     CDS             complement(<1..214)
                     /locus_tag="VNN25_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HXH90887.1"
                     /translation="MFFHTIDQVRRFFKILGGFTLLGLGAVMLVTPGPGWLVIFLGLT
                     LLAAEFVWARRLMDRMKEQGTRLKDSV"
     gene            complement(664..1179)
                     /locus_tag="VNN25_04845"
     CDS             complement(664..1179)
                     /locus_tag="VNN25_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXH90888.1"
                     /translation="MEAQQIFVNQLSGTVSRKILQNVVGTLSSMLKTAQYWGYICEGI
                     SFTKLVLQERAITRPSKFFAAEPVRAILAISQGWHHVMLAIAAMTGLRIGETLALKVS
                     DFDFERKLLTVHRSVWRGKLSIPKTPTSQAVLPLPDTLVRIVREHFATLKSEWLFLNI
                     YGGFLTSANVA"
     gene            complement(1614..2429)
                     /locus_tag="VNN25_04850"
     CDS             complement(1614..2429)
                     /locus_tag="VNN25_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HXH90889.1"
                     /translation="MTLDIWLESHPYLQAVADFHAQVAMAAASIPSGSAQIPNWDNYR
                     GDYQAGIPLLHSARAAIDFEPAEAILVSLVERMASILLPEKLALECRDSDDQLRSERN
                     APGRAVAWLLGGEAFEPAHPGLLRYLGWTAFAKYLRPLGDAFGTWREEEQWLRSYCPT
                     CGTLPAMAQLVGTDPGRLRLLSCGCCRTRWQFLRIGCPFCERTDDHRLSALAIEGENY
                     LRIDYCQSCRAYLKTYNGEGSEPLFLADWSSLHLDIIARDRGLKRLANSLYEL"
     gene            complement(2452..4704)
                     /locus_tag="VNN25_04855"
     CDS             complement(2452..4704)
                     /locus_tag="VNN25_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXH90890.1"
                     /translation="MPRQRDQGRPPDHTKIQFPEGARMTNAAAVGPIRATLIDITNCI
                     GCRACQVACKQWNEREGEETELEAELGFQNPATLSAKTYTLIAFHEMENPQKPGGVES
                     AFVMQRCLHCLEPACASACPTTALYRQADGPVTYDVDKCIGCRYCMLACPWDVPAAEW
                     NSLAPKISKCTHCADRADQPVPIAFNGKSLSNDAGKRFIESIATPACVKACPADALRY
                     GTRDEMLALAHKRISDRPDKYVDHIYGEKELGGTSVVYLSAVPFEKLGFPKYGEKPFP
                     AFTSTALGAVPPAVMTVGALLGATYAFFRKRLQAVSDGSAAAKHSGDHGHVEFESLPN
                     KLMTPFNWVLLLLMAFGGASLVARFALGLGGSTHLSNTYPWGLWILFDLVWIAVAAGA
                     FAMAGVIYVLQRKDLYGLGRTAVLMGLLSYSFVMVTLLADLGLPWHSYQLALQAPGHS
                     AMFEVSWCVGLYVTILLLEFLPVPFERWRLHRAAAAWRRWNGVYVAAAVTLFVYLLSR
                     SLVYAFVTAVIFGALAWMFRARDEKPEPIMLAIAAVTLSTMHQSSLGSLYLLMPNMLA
                     PQWWSPVMPVSFLLSSIAAGTALVILIDMWIAKGWRRPREITRLASVGQIAFWSLLAY
                     IVFHLGDMAIRNQFSGAFAGRLGLAFAAEIVLGGVIPLILLARRSFRQRPGVLFVAAL
                     LTVLGVTYNRMNVVLFAMTFRGRMPWRAAESYAPSVVEWGISIGLIAATIFLFGLGAR
                     LMPVLPREEA"
     gene            complement(4632..>6658)
                     /locus_tag="VNN25_04860"
     CDS             complement(4632..>6658)
                     /locus_tag="VNN25_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXH90891.1"
                     /translation="DDPHCIFSRLKTFVSRYTLETGEQITGIPAEQIKQIAEMMAKNR
                     PGSILYALGMTQHTTGVQGIRAFTILQLLLGNIGKPGGGVNALRGEPNVQGACDMAVL
                     YNYMPGYLNSASNGEPTIHDYVRKNGIADSRYLVNTLKAFFGGAASAENDYAYAWLPK
                     RNASKDYGTLPMFEDALAGKLKLLWIVGQNPAVTVPNLRLIFDAMAKLETLVVQEIWE
                     TETAAFWRRPGVDPKSISTEVILLPAAFFMEKNGTISNSGGMVQWRHAAVKPPGQAKP
                     DGEIVDLVFRRVRDLVKDSDEPRDEIIQKAFWNYTTAEDVLREINGYALRDNPETGLK
                     KGDLVRKISDLRADGSTSSGAWIYAGIFAQGENLSKRRDSRTDPGGLGLYPGFGWTWP
                     NNMRILYNRASCDRNGKPYPGSKSIVWWDKQAKRWTGYDVPDVPVATDGPDTPNGQRA
                     FHLGAEGVGRLFAAVYADPDEKHPGYPRDVAYVPKDGPLPEMYEPVESPVENILHPRV
                     KSNPMLKYPRVATHQPIGTVDKYPYVLMTSTVAEHWCGGSTTRNIPWLNELVPEPVVE
                     IPVALAQKLGVNSGDWVRVSSARGELEVKALVTPRMKELKIGDRQVTVVWMPYNWGYQ
                     GLSTGPSVNHITIDAADPGAGTQETKACLVNVIRAGRPTTRRSSSPRGRA"
BASE COUNT         1353 a         1969 c         2026 g         1310 t
ORIGIN      
        1 aaaccgaatc cttcaatcgc gtgccctgct ctttcatccg gtccatcaat cgccgtgccc
       61 acacaaactc tgcggccagc agtgtcaggc cgagaaaaat gaccagccag ccgggcccgg
      121 gtgtgaccag cataaccgct cccagcccca gaagcgtaaa ccctccgaga attttaaaaa
      181 agcgccggac ttgatcgatg gtgtgaaaga acataggccc tcatgggcag aggcggggga
      241 atcctgattg tgactggaac tcctgtggca atcaacaacc acattatttg gatgcgtaac
      301 cgagcgaaaa gggcatcgat tccgcaaacg agccgcaaaa aaattgcggc gaaagtggga
      361 ttcgaaccca cctgcggatg cccgcaaatt actggtttaa ctgactcacg gtgattcggt
      421 taggtccatc caggtccgga accgactcga ccttctcgac gaccgcccgc cgtgattcca
      481 cctggacatg gctgtataca ccaatcgtga cgcgtgggtc tgcatgccgg agctgcgttt
      541 gtgcctcctg tggcgttgct ccgctgttcc gttgtagaga agcagcgtcg gatgggtatg
      601 ccggaatgca tggaacccac agcgcggaag accaagacca tcgaggatcg gcgacagcac
      661 gtgttacgca acatttgccg aagtcaggaa gccgccatat atgttcagga acagccattc
      721 gcttttcagt gtcgcgaagt gctcgcgaac gatccgcacc aacgtatcgg gcaaagggag
      781 gaccgcttgg ctggttgggg tttttgggat gctcagtttc ccgcgccaga cggagcgatg
      841 cacggtgagc agtttccgtt cgaagtcgaa atcgctgact ttcagcgcta gagtttcgcc
      901 gatccgtaga cctgtcatgg cagcaatcgc aagcatcacg tggtgccagc cttgcgatat
      961 tgccagaatg gcacgtaccg gctcggctgc gaagaacttg gatggtctgg taatggccct
     1021 ctcctgaagc accaactttg taaaactgat gccttcgcag atgtaacccc agtattgtgc
     1081 ggttttgagc atagaagaga gtgtgccgac aacgttctgc agaatcttcc gtgaaacagt
     1141 tcccgagagc tggttgacga agatctgttg agcctccaat cccaactcat tcagtcgcgt
     1201 cttgccgaga atgggcagga tttaattcct caggtgtgac tccgccgcat ggatggtcga
     1261 agcttttcgt ttcgaaagaa cttcctgttt ccatcgttcg gcgaattctg caacggttgc
     1321 gacccggccc ggacgatagg ttagcgaatt gatcggggcc aaaagcactt ccaattgtcg
     1381 ttccgctaat cgcttcgtgg gaaactcgtc tctcgacccc aacagtgtgg atcgccgaat
     1441 gcgggcttcc tcgccgtcca ggcagatgac gattcaagcc attgtcgggg cggacttcta
     1501 tgtggactgc gaagcgaggg aaagtattca gcgcacagca tttctacact acaactggat
     1561 ttcctttctt agcttctgtc acccgtccgt tacaagatcg aagctgggcc tggtcacagt
     1621 tcgtagagtg aattagctaa ccgtttcagt ccacgatcac gggcgataat gtcgaggtga
     1681 agcgaactcc aatccgccag aaagaggggc tcgcttccct ctccgttata ggttttgaga
     1741 taggcgcggc aagactggca gtagtcgatg cgaagataat tctctccctc gatcgccagg
     1801 gcggaaagcc gatgatcgtc tgtcctttcg caaaacgggc acccaattcg caggaattgc
     1861 caacgcgtgc ggcaacagcc gcaagaaagg agacgcaatc ggccgggatc ggttccaacc
     1921 agctgcgcca ttgcgggcag cgtaccgcaa gtgggacagt aactgcggag ccattgctct
     1981 tcctcgcgcc aggttccgaa agcatccccc agtgggcgca gatatttggc gaacgcagtc
     2041 caaccgaggt agcggaggag acctgggtgg gctggttcga aagcctcgcc gccgagtaac
     2101 caagcgaccg ctcggcccgg cgcattccgc tcgctgcgca attgatcgtc cgaatctcga
     2161 cactccaagg ccaatttttc tggcaatagg attgaagcca tcctctcgac gagcgatacc
     2221 agaatcgcct cagcgggctc gaaatcgatc gccgcgcggg cgctgtgcag gagagggatg
     2281 cccgcctgat aatcaccccg gtagttatcc cagttcggaa tctgcgcgct accacttggg
     2341 atgctggctg cagccatcgc aacttgagcg tgaaagtcag cgactgcttg caggtacgga
     2401 tggctctcca gccagatatc tagggtcatg gaatggatat ccccatcctt ctcaagcctc
     2461 ttcccttgga aggactggca tgagtcgtgc gccgagtccg aaaaggaaga tagtcgcggc
     2521 gatcaagccg atcgaaatcc cccactcgac gactgacggc gcataactct ccgctgcgcg
     2581 ccagggcatt cggcctcgaa aagtcatcgc gaagaggacg acgttcatcc ggttgtaagt
     2641 cacgccgagt accgtgagta aggccgcgac gaagagcacg ccgggacgtt gccgaaacga
     2701 cctgcgggcc agcaagatga gcggaatcac gccgccaagg acgatctcgg ccgcgaaggc
     2761 caggccgagc cttcctgcaa aggcgcctga gaattggttg cgaatcgcca tgtctccgag
     2821 gtgaaacacg atgtaggcga gaagcgacca gaatgcgatc tgtccgaccg aggcgagccg
     2881 cgtaatctcg cgcggtcgac gccatccctt cgcgatccac atgtctatga ggatcacaag
     2941 cgcggtgccg gctgcaatcg acgacaacaa gaacgagaca ggcatcacgg gagaccacca
     3001 ttgcggggcg agcatgtttg gcatcaacaa gtacagggaa ccgagcgagc tctggtgcat
     3061 ggttgatagc gtgacggcgg cgatcgcgag catgatcggt tctggttttt cgtcgcgcgc
     3121 gcgaaacatc caggcgagtg cgccgaagat cacggcggtc acgaaggcgt agactaggct
     3181 gcgcgaaagc agatagacga acagtgtcac ggccgccgcc acatagactc cattccaccg
     3241 ccgccaagcc gctgcggcac gatggagtct ccaacgctcg aacggaaccg gcaggaactc
     3301 gagcaggagg atcgtgacgt agaggccgac acaccacgag acctcgaaca tggccgagtg
     3361 gcccggagcc tgtagggcga gttggtagga gtgccagggc agaccgaggt cagcgaggag
     3421 agtgaccatc acgaaggagt agctcaagag gcccattagg acggctgtgc gacctagccc
     3481 gtacaggtct tttcgctgga gcacatagat cacgccggcc atggcgaacg cgcccgcggc
     3541 caccgcgatc catacgaggt caaagaggat ccacaaaccc cacgggtatg tgttggacaa
     3601 gtgagtgctg ccgccgaggc ccagggcgaa gcgcgcgacc aacgaggcgc cgccgaatgc
     3661 catgagcagg aggagcaccc agttgaaggg agtcatcagc ttgttcggaa gagactcgaa
     3721 ttcgacgtgg ccgtgatctc cggaatgttt ggctgctgcc gatccgtctg acaccgcctg
     3781 gagccgcttc cggaagaagg cgtacgtggc accaagcagg gcgcccacgg tcattacggc
     3841 agggggaaca gcgccaagcg ccgtactggt gaaggccgga aagggcttct ccccatactt
     3901 ggggaaaccg agtttctcga agggcacggc cgaaaggtac accacgctcg tcccgcccag
     3961 ttctttttct ccgtagatgt ggtcgacgta cttgtcgggc cggtcggaga tccgcttgtg
     4021 cgcgagcgca agcatttcgt cccgagttcc gtagcgtagg gcgtcggcgg ggcacgcctt
     4081 gacgcaggcg ggagtcgcga tgctttcgat gaatcgcttg cccgcgtcgt tggacagcga
     4141 cttgccgttg aacgcgatcg gcaccggctg atcggcgcgg tcagcgcagt gggtgcactt
     4201 cgagatcttc ggggcgagag aattccactc cgcagccggc acatcccatg gacacgccag
     4261 catgcagtag cggcagccga tgcacttgtc aacgtcgtat gtcactgggc cgtcggcctg
     4321 ccggtagagc gccgtcgtcg ggcaagcgga ggcgcaggct ggctccagac agtggagaca
     4381 ccgctgcatc acgaacgcgg attccacgcc gcccggcttt tgcgggtttt ccatctcgtg
     4441 gaacgcgatc agcgtgtatg tcttcgcgct gagcgtcgcc gggttctgga agcccaattc
     4501 agcctccagc tcagtctcct cgccctcgcg ttcattccac tgcttgcagg ctacctggca
     4561 cgcccggcat ccgatgcagt tggtgatgtc gataagcgtc gccctgatcg ggccaacggc
     4621 tgcggcgttt gtcatgcgcg ccccctcggg gaactggatc ttcgtgtggt cgggcggccg
     4681 gccctgatca cgttgacgag gcacgccttg gtttcctggg tccccgctcc aggatccgcg
     4741 gcgtcgattg tgatgtggtt gacgctcggg ccagtcgata acccctgata tccccagttg
     4801 tacggcatcc agacaacggt gacttgccga tcaccgattt tcagctcctt catgcggggc
     4861 gtgacgaggg ctttgacctc gagctcaccg cgcgccgacg agaccctcac ccagtctccc
     4921 gaattcaccc caagcttctg cgcgagggca accggtatct ccaccaccgg ttcggggact
     4981 agctcgttga gccatggaat gttcctagtc gtcgagccgc cgcaccagtg ttcggcgacc
     5041 gtcgacgtca tcagcacata cggatacttg tcgacggttc cgatgggctg atgcgttgcg
     5101 acacgcggat acttgagcat cgggttgctc ttcacgcgtg ggtgaaggat gttttccacc
     5161 gggctctcga cgggctcgta catttccggc agcggaccgt ccttaggtac gtaggccacg
     5221 tcgcgcggat atcccggatg cttctcgtcc ggatccgcgt aaaccgccgc gaacaggcgc
     5281 ccgacgccct ccgcgcctag gtggaaggct cgctggccat tcggcgtgtc cgggccatcg
     5341 gtcgcgacgg ggacgtccgg cacgtcgtat cccgtccagc gcttcgcctg cttgtcccac
     5401 cagacgatgg atttggagcc tggataaggc ttgccgttcc gatcacacga cgcccggtta
     5461 tagaggatgc gcatgttgtt gggccaggtc cacccgaaac caggatagag accgaggcct
     5521 ccgggatccg ttcgcgagtc gcggcgtttc gaaaggttct caccctgcgc aaaaatgccc
     5581 gcatagatcc acgcgccgga cgacgtggaa ccgtcggctc gaagatcgct gatcttgcgg
     5641 acgaggtcgc ccttctttag gcccgtctcc gggttgtcgc gaagggcgta cccgttgatc
     5701 tcgcgcagga cgtcctcggc cgtggtgtag ttccagaagg ccttctggat gatctcgtct
     5761 ctcggctcgt ccgagtcctt gaccagatcg cgcacccggc ggaatacgag gtccacaatc
     5821 tcgccgtcgg gtttggcttg ccccggaggc ttcacggcgg cgtggcgcca ttgcaccatg
     5881 ccacccgagt tgctgatggt accgttcttt tccatgaaga aggccgcggg cagcaggatc
     5941 acctcggtgg aaatcgattt cgggtcgacg ccgggacggc gccagaaggc tgcggtctcg
     6001 gtctcccaga tctcctgtac cacgagcgtc tcgagcttcg ccatcgcgtc gaatatcagc
     6061 ctgaggttgg gcacggtgac tgcgggattc tggccgacga tccagaggag cttcagcttg
     6121 ccggcgaggg catcctcgaa catcggcagg gttccgtagt ccttggaagc gttccgcttg
     6181 ggcagccatg cataggcata atcgttctcg gcgctggccg ccccaccgaa gaacgccttg
     6241 agcgtgttga caaggtaccg actatcggcg atcccattct tgcgcacgta gtcgtggatg
     6301 gtcggctcgc cgttcgatgc agagttaaga tagcccggca tgtagttgta gagcaccgcc
     6361 atgtcgcagg cgccctgcac attcggctcg ccgcgcagcg cgttgacgcc gccgcccggc
     6421 ttgccgatgt tgccgagcag aagttgtagg atagtgaacg cgcggatccc ctgcacgccg
     6481 gtggtgtgct gggtcatccc gagcgcgtag aggatggatc ccggccggtt cttcgccatc
     6541 atctccgcga tctgcttgat ctgttcggct ggaatgccgg tgatctgctc gcccgtctcc
     6601 agcgtgtaac gtgagacgaa ggtctttagg cgggagaaga tgcagtgcgg gtcatcca
//
LOCUS       DATLVW010000149         7996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_174117, whole genome shotgun sequence.
ACCESSION   DATLVW010000149 DATLVW010000000
VERSION     DATLVW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174117"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VNN25_04865"
     CDS             <1..139
                     /locus_tag="VNN25_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXH90892.1"
                     /translation="GNAGRRVGYGSASQFSREYSRFFGSAPARDVNRLREEGLTTTDS
                     R"
     gene            complement(348..3326)
                     /locus_tag="VNN25_04870"
     CDS             complement(348..3326)
                     /locus_tag="VNN25_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HXH90893.1"
                     /translation="MLTFATAGSQETVVVRNSAAILNDIVAFSTGSGLNFKSRRQSVD
                     SMRGHEHQPRRAHIWAHTAAGCCALLLTLAAHADPFVRPVVSGRQLLIGGVPFELRSF
                     AYSPVPIGENPAASTSTRYTDARLIRRDGPLMTAAGANGIRIFDAMTIKSDGTGEVHT
                     NLAFIREAALQGLWVVMGTGFPCDLDFANATIRNNVVQAHVNLAAQFGAEPNVLMWAP
                     GNEMNLAIAHSHFADWYSLLEAISSAVKTAQGPGGPLLCGDNGDVSSVDDACPTSVFS
                     NGGATAGMTLAPSVDVWAVNLFRGASLGTLREELLAAPAKPYWIAEMGIDSFNNTLQV
                     QDEASQAAVLKALWDQIRVFDDVLFGADLGFWSDEWWKCGAPTVQDNCGNPFGNGPDN
                     FINEEHLGAMRIAAGAQGEVNQVIPKQAYTASQTAWADACPNALGPEPFLADFDAGTS
                     NNYAGFSFGQQNGIGVTSGNTAAGASGAANDFALRVAQASVPGATPYIIAVLTLFPPS
                     TNTGYDFSSFDTLSFDIRKGTSTAFGSWLVRLEDSSDPPNEFMNDSVALSALTTSFQH
                     IQIPLTSFTAPSKPVDLTRLAQIVFAAVLPGSRPSSLEIDFILDNVRIYSSSAPASCN
                     RTPTGIALSPSSLVEGTPNSTVVGAFSTTDPDAADSHTYTLVNDPSNGGFGLSSANLV
                     VANTSLLTAAQSPYLLSVRSADNGSPSLAVERFVSVQVCAYQISPVGTNVSSASGSGS
                     IQVTATTSCSWTAAAPAGSFVTISSGSSGTGSGTVQYSFTPNGSGSLRSTTLTIAGKS
                     FTLSQNSSGVSMSLAATASSSQVALSWSAASGATSYSVMRSANNSAFSSVITTSNLFY
                     TDASVVNGTGYRYEILALANGPVSMGYSNIELVVPFAWTDPSLTAGTIVKAAHLAELR
                     SAVNAARATLGWQPLTFNGTIAGGQTVGRNHLVDLRSAVDAVRAGAGEASASYSDPTV
                     TVNVTPIRAPHILDLRAALQ"
     gene            complement(3970..4986)
                     /locus_tag="VNN25_04875"
     CDS             complement(3970..4986)
                     /locus_tag="VNN25_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXH90894.1"
                     /translation="MQTYAAIDLHSNNGLLVVADETDRVLRQKKLANKLELFVAELEP
                     FRATLQGVAVESTFNWYWLVDGLLKHGFPVMLVNTAAIQQYEGLKYTDDPYDARWLAH
                     MMRLGILRTGYIYPREERAVRDLLRKRASLVQQRTANILSVQNLEQRNRGAKISSREV
                     KQLTEERIGELYDNEDLGLALTTTIKVIEALTEQIRCLEKAVLKKARLRDEFKLLLTV
                     WGVGDILGLTIMYEAGDMSRFADVGPFASYCRCVQAQRLSNGKKKGENNGKNGNRYLA
                     WAFVEAAHYAIRNYEGAKRFYDRKAAKTLPVVAIKAIAHKLARASYHVLRDQKPFDPK
                     KVFP"
     gene            complement(5198..7657)
                     /locus_tag="VNN25_04880"
     CDS             complement(5198..7657)
                     /locus_tag="VNN25_04880"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH90895.1"
                     /translation="MNHRVPFLLAALACTTFVQGQQTQLSPGVGNAVLLARNSVQVDN
                     TVTIVSGDVIVNDATVGPVLGELALSLDRAVITPAGYKIAGTSIDLDSGAVVGGDAYY
                     NALTNQGTIAGTQFSPLALPVFAGFPPALARPPGSGNVTVAANTSLTLGEGAYGNLTI
                     NKGATLLLSGGGYAFQSITVASGGSIRYAAHADIVVSGRADFGANDVVSPADGSGLTP
                     AAMRIQVDGINGTTGVLGATPPSIHVGQSSKISATLYATAGSLILDQSVEGNGAFVGR
                     DILVGHNSKLTLNSAYNQPPSANAQVVFTTGSDPLPITLTGTDPEGGTLTFSIVSAPH
                     AGTLSAPVSASPTSANVTYTPSAANVADSFTFRVTDPAGATGDAVVTINPTAQEPPPP
                     PPTTVIATDSSAQTAQDVAATLTLSGTPPAGVALTFSIVPSTGPFHGSLGAVSQGTEV
                     PERSATVVYTPDAGYTGPDSFQFQACGTIASVNTCDAASFSITVGTAPSDPPSIAHDV
                     QVSTFANTSVLVSLGDSSITTASRHFIIKPMAATLQPVAIAGNVADSNGDQLGDNVNA
                     LPGPAPVFMSAGVNQGGTAGSGGTTRMQFEWDMTGISGSLSNLQSADVILPTNRGTVD
                     SLDTFFYAAGVSGDGLLTNSDFEAPAEQIPGVVMPVPPSMPIGSDGTFSFSVLGPIHT
                     AAQNGFSFFAIQGRVNESLTGPARGLQVRTTADGNISSGNIPKLSLTTPGVTAPLTYR
                     ITSLPTSGVLRDSANALITTVPYDLPSAQVSYTPNTGFLGTDTFSFSVSNGVTTSNAL
                     GTISVFIPNCQTNPAGCNDGR"
BASE COUNT         1464 a         2271 c         2692 g         1569 t
ORIGIN      
        1 ggggaacgcg ggccggcgcg tcggttatgg gagcgcgtcc cagttcagcc gcgagtacag
       61 ccgcttcttc ggcagtgcgc ccgcgagaga cgtcaatcgc ttgcgcgaag agggattgac
      121 gacgacagac agccgctgaa agtcgtgaac ctcaacttgg agttgtcaac ctgaagttga
      181 ggtgatccgc tcttgaattg aggtgtctcg acgtcaattc aaggttggga gtcctcaaat
      241 tgaggttatc aaacctgact ttgaggttgc tgaatctcaa tctaggttga ccccctaacc
      301 agcggccgga ggctgctcgt ggggttctcg aacatcggtc cacgacatca ttgcaatgcg
      361 gcgcggagat cgaggatgtg cggggcgcgg atcggggtga cgtttacggt cacagtgggg
      421 tcggagtatg aggcggaagc ctctcctgct cctgcgcgca ctgcgtcgac ggctgagcgg
      481 agatcgacga gatggttgcg tcccacggtc tggcctccgg caattgtccc gttgaaggta
      541 agcggctgcc agccgagggt ggcgcgggcg gcattgaccg cgcttcgcaa ctcggcgagg
      601 tgagctgctt tcacgatcgt tccggcggtc agcgacggat cggtccaggc gaaggggacg
      661 accagctcga tattgctgta gcccatcgag accgggccat tggccagcgc cagaatctcg
      721 taacgatagc cggtcccatt cacgaccgaa gcatcggtat agaacaggtt ggatgtcgtg
      781 atcacgctgc tgaaggcgct gttgttcgcg ctcctcatga cggaatatga cgtcgcgccg
      841 gatgccgcgc tccaggagag tgcgacctgc gaggatgacg ccgtcgcagc gagactcatc
      901 gatacgccgc tggagttctg ggaaagggtg aaactcttgc cggcgatcgt cagcgtcgtt
      961 gatcgcaagc ttccgctgcc gttcggggtg aatgaatact gcactgtgcc actccctgtt
     1021 ccggacgagc ccgagctgat cgtgacgaag ctgccggccg gagcggccgc cgtccaggag
     1081 caactcgtcg tcgccgtgac ctggattgag ccgctgccgc tcgccgagga gacattcgtc
     1141 ccgaccggtg agatctggta ggcgcaaacc tggacgctga caaaccgctc cacggcgagc
     1201 gatggcgagc cgttgtcggc ggatcgcacg gagagcagat acggggactg ggccgcggtc
     1261 aggagggagg tgttggcgac gacgaggttg gcgctcgaga gtccgaatcc gccgttcgac
     1321 gggtcgttga cgagcgtgta ggtgtggctg tcggccgcgt cggggtcggt cgtcgagaaa
     1381 gcgcccacga ccgtgctgtt tggcgtgcct tccacgagac tggaggggct cagggcaatg
     1441 ccggtcggtg tccggttgca ggacgccggc gcggaggagg agtagatgcg gacattgtcg
     1501 agaatgaaat cgatctcgag actcgaagga cgggagccgg gcaggacggc ggcgaagacg
     1561 atttgggcca aacgggtgag atccacgggc ttggagggcg cggtgaaact cgtcagcgga
     1621 atctggatgt gctggaacga agtcgtgagc gccgagagcg cgacgctatc gttcatgaat
     1681 tcgttcggag gatcgctgga gtcctcgagg cgaacaagcc acgagccgaa ggcggtgctc
     1741 gttccctttc ggatgtcgaa ggaaagggta tcgaaggagc tgaagtcgta gcccgtgttg
     1801 gtggacggag ggaagagcgt gaggacggcg atgatgtaag gggtggcgcc gggaacggag
     1861 gcttgcgcga cgcgcaacgc aaaatcgttc gcggctccgg atgcgccggc agcggtgttc
     1921 ccggaagtca cgccgattcc gttttgctgg ccgaacgaga agccggcgta gttgttggaa
     1981 gtaccggcgt cgaagtccgc caggaatggt tccggtccga gggcgttggg gcaggcgtcc
     2041 gcccaggcag tttggcttgc ggtgtacgcc tgtttaggga tcacttgatt gacctccccc
     2101 tgagcgccgg cagcgatcct catcgcgccg aggtgctcct cgttgatgaa gttgtcaggt
     2161 ccgttgccga aagggttccc gcaattgtcc tgtacggtcg gagcgccaca tttccaccat
     2221 tcatcgctcc agaaaccgag gtcggcgccg aagaggacgt catcgaagac gcggatctgg
     2281 tcccaaagag ccttgagtac cgcagcctgc gacgcttcgt cctgcacctg gagggtattg
     2341 ttgaaactgt cgatacccat ttcagcaatc cagtaaggct tggcgggagc agccaggagc
     2401 tcttcgcgca gcgtccccag cgaagcccct cggaagagat tgactgccca gacgtcgacg
     2461 gatggggcga gcgtcatgcc ggccgtagcg ccgccgttgc tgaagacgga ggtgggacaa
     2521 gcgtcatcga cgctgctcac gtcgccattg tcgccgcaga gcagagggcc gcccggcccc
     2581 tgggcagtct tgacggccga ggagatcgct tcgagaaggc tgtaccagtc ggcgaagtga
     2641 ctgtgagcga tagccagatt catctcgttg ccgggtgccc acatgaggac gttcggctcc
     2701 gcgccgaatt gcgcggccag attgacgtgc gcctggacga cgttgttgcg gatggtggcg
     2761 ttggcgaagt ccaggtcaca ggggaagccg gttcccatga cgacccacag gccctgaagc
     2821 gcggcttcgc gaatgaaggc gaggttggta tgcacctcgc cggtcccgtc ggacttgatg
     2881 gtcatggcgt cgaagatacg aatgccgttt gccccggccg cggtcatgag cggcccatct
     2941 cggcggatga gacgagcgtc ggtgtagcgc gtgctcgtcg aggcggcggg attctcgccg
     3001 atggggacgg gagagtacgc gaaggagcgc agttcgaagg gaacgccgcc gatcagaagc
     3061 tgacgtccgg aaacgacggg gcgaacgaag ggatcggcat gcgccgcgag agtcaggaga
     3121 agcgcgcagc agccggccgc ggtgtgcgcc caaatgtgcg cacgacgagg ttgatgttcg
     3181 tgtccgcgca tgctgtcgac ggactgcctc ctgcttttga aattcaaacc actgccggta
     3241 ctgaaagcca cgatgtcatt caggatggcg gcgctgtttc ttacgacgac ggtctcctgg
     3301 gagccggccg ttgcaaacgt cagcacactc ggagctcgtc atccgcaggt tttcgttcgg
     3361 cgatccagac cttcatagtt gtctctccag tctgagtgga tgacttcagc catggcgccg
     3421 ccgcaagcgc cgatagcgaa cttgaacagt tcctgtgagt agtcaccggc caaaggacaa
     3481 cctgagagtt gatatgtatg tccgtaccgc aggaggtgcc agcggcgaaa acggaaaatg
     3541 gggtcaccca ttagcaggcg ttgtcaatat gggcgggcgt tgcccttagc tgcgtctagc
     3601 agctgtgatt gatggtggtc ctttcgaggt ggcgagatga tttggcagtc ccggtgtttc
     3661 agtcgagcgg tgtcggatga atccgcacca gtcacccatc aggatcaagg ctggggagtc
     3721 gtccgaagag cggctccggc ccctgacgca caaggcgtcc cagaaacgga tcggtcatac
     3781 gagcgcgtgt ccaattggaa ggtgcacaga actcgtggct caccggccga gaccggcgtc
     3841 caaccccttg tggctcacgc gctcgtcgcg aaatcataac gtcaactcat cctggccggt
     3901 gcggcctcca atcaggtcgc tggttgtgaa ccaaccccaa agtggtggct gaccgcaccg
     3961 accaagctct caagggaaca ctttcttcgg gtcgaacggt ttctggtcgc gcagcacgtg
     4021 gtacgaggcg cgagcgagct tgtgggcgat ggccttgatg gcgacgaccg gcagggtctt
     4081 tgctgctttc cggtcgtaaa agcgcttcgc tccctcgtag ttacgaatgg cgtagtgagc
     4141 tgcttcgacg aaggcccagg ccagatacct gttgccgttc ttcccgttgt tctcgccctt
     4201 cttcttgccg ttggacagac gttgcgcttg cacgcagcgg cagtacgaag cgaacggtcc
     4261 gacgtcggcg aagcgactca tgtcgccggc ctcgtacatg atggtcagcc cgagaatgtc
     4321 gccgacgccc cacaccgtca gtagcagctt gaactcatct cgcaagcgcg ctttcttgag
     4381 cacggccttc tccagacagc ggatctgctc ggtcagcgcc tcgatcacct tgatcgtcgt
     4441 ggtgagcgcc agccccagat cctcgttgtc gtagagctca ccgatacgct cttcggtgag
     4501 ttgtttgact tctctcgatg agattttcgc tcccctgttt ctttgctcga ggttctgaac
     4561 gctcaggatg ttggccgtcc gttgctggac caggctggcg cgctttcgca gcagatcgcg
     4621 aaccgctcgc tcctctcgcg gatagatata tccggtgcgc aggatgccca ggcgcatcat
     4681 gtgagcgagc caccgcgcgt cgtaggggtc gtccgtgtac ttgagtcctt cgtattgctg
     4741 aatcgctgct gtgttgacga gcatcaccgg aaagccgtgt ttgagcagcc cgtcgacgag
     4801 ccagtaccag ttgaaggtcg actccacggc cacaccttga agcgtcgctc ggaatggttc
     4861 gagctcggcg acgaacagtt ccagcttgtt cgccagcttc ttctgcctca gcacccggtc
     4921 ggtttcatcg gcgactacca gcagcccgtt gttcgaatga agatcgattg ccgcatacgt
     4981 ttgcatcgca agcctccttt gtccacgatg ctatgaccac gcagcgggga ctgggttgtg
     5041 cctgctatat gcgtatcagg tctgaagtgt gaagtgacgg aagaacgctg gtcgcccgaa
     5101 gccacgaccc gccttccgtc acttcacact tcagacctga ccccattccc acgcgggatt
     5161 cggacccgcg tggttttttt gcgtcgatat cgctgcctta ccttccgtcg ttgcagcctg
     5221 cggggttggt ttggcaattg gggatgaaga cgctgatcgt gcccagggcg ttgctcgtgg
     5281 tgacgccgtt gctgacggag aacgagaagg tgtcggtgcc gaggaagccg gtgttggggg
     5341 tgtagctcac ctgggcgctc ggcaggtcgt acggaactgt cgtgatcagc gcgttggcgc
     5401 tgtcgcgcag gacgccgctc gttgggagcg aggtgattct gtaagtcagc ggcgcggtga
     5461 ctcccggggt agtcaacgac aacttgggaa tgttgccgct gctgatgttt ccgtccgccg
     5521 tcgtgcgcac ctggagtccg cgtgccggtc cggtcagcga ttcgttcacg cggccctgaa
     5581 tggcgaagaa gctgaaaccg ttttgggccg cggtgtgaat cgggccaagt acgctgaagc
     5641 tgaaggtgcc atcgctgccg atcggcatcg acggcggtac cggcatgacg acgccgggga
     5701 tctgttcggc cggagcttcg aagtccgagt tggtgagaag gccgtcgccg ctcacgcctg
     5761 cggcatagaa gaatgtgtcg agcgagtcga cggtgccgcg gttcgtgggc aggatcacgt
     5821 ccgcgctctg aaggttgctt agcgaaccac tgatgccggt catatcccac tcgaactgca
     5881 tacgcgtggt tccgcccgag cctgcggtgc cgccctgatt cacaccggcg ctcatgaaga
     5941 ccggcgccgg gcccggaagc gcgttgacgt tatcgccgag ttgatcgccg ttggagtcgg
     6001 cgacattacc ggcaatcgcg acgggttgga gcgttgccgc catcggtttg atgatgaaat
     6061 gtctgctcgc cgtcgtgatg gagctgtcgc cgagcgagac gagaacgctg gtgttcgcaa
     6121 aggtcgatac ctgaacatcg tgagcgatgc tcggcggatc ggacggcgca gttccgaccg
     6181 tgatcgagaa cgacgcggca tcgcaggtgt tgacgctggc gatcgtgccg catgcctgga
     6241 actggaagct gtccggtccg gtgtatccgg catcgggcgt atacacgacc gtcgcgcttc
     6301 gttccggaac ctcggtgccc tggctgaccg cgccgagcga tccatggaac gggccggtgc
     6361 tcggaacgat cgagaaggtg agcgcgacgc cggccggagg agtgccgctc agcgtaagcg
     6421 tcgcggcaac gtcctgcgca gtctgcgcgg aactgtcggt cgcgatgacc gttgtcggag
     6481 ggggtggagg cggctcctgc gccgtcggat tgatcgtcac gaccgcgtcg ccggtggctc
     6541 cggcaggatc ggtcacgcgg aacgtgaagg agtcggcgac gttggccgcc gacggtgtgt
     6601 aggtaacgtt ggcgctcgtc ggcgaagcag acaccggcgc ggagagcgtc cccgcgtgcg
     6661 gtgcggagac gatgctgaac gtcagcgttc caccctccgg atccgtcccg gtcagcgtaa
     6721 tcggcagcgg atccgagccg gtcgtgaaga cgacttgcgc attcgctgac ggaggctggt
     6781 tgtaggcgct gttgagagtg agcttgctgt tatggccgac gagaatgtcg cgcccgacga
     6841 aggcgccgtt tccttcaacg gactgatcga ggatgagcga gcccgccgtg gcgtagagag
     6901 tcgcggagat tttgctggac tgtccgacat gaatggaggg aggcgtcgcg ccgaggacgc
     6961 cggtcgtgcc gttgatgccg tcgacctgaa tacgcatcgc cgccggagtg agtccggaac
     7021 cgtcggccgg cgagaccacg tcgttggcgc cgaagtccgc gcgcccgctg acgacgatgt
     7081 cggcgtgtgc ggcataacga atcgatccgc cgcttgctac ggtgatcgac tggaaggcat
     7141 acccgccacc gctcaggagc agcgtcgccc ctttgttgat ggtcaggttt ccgtacgcac
     7201 cctcgcccag cgtgagcgaa gtattggcgg caacggtcac attgcccgaa ccgggcggac
     7261 gagccagcgc cggcggaaac cccgcgaata cgggcaaggc cagcggcgag aactgcgtcc
     7321 cggcaatcgt gccttgattc gtcagagcgt tgtagtaggc atcaccgccc accaccgcgc
     7381 cggagtcgag atcgatcgat gtgcccgcga tcttgtagcc ggccggcgtg atgaccgcgc
     7441 ggtcgagcga cagcgctaat tcgccgagga ccggaccaac ggtggcgtcg ttcacgatca
     7501 cgtcgccgga gacgatggtc acggtgttgt cgacctggac gctgttcctc gccagaagaa
     7561 cggcgttgcc gacgcccggc gagagttgcg tttgttgccc ctgaacaaac gttgtgcagg
     7621 cgagtgccgc cagaagaaac gggacacgat ggttcatcgc gtgatcctca tctgagttgt
     7681 ttccggggtt gtaggtttcg ggttgaccgt caatgtacgt cctgttgggt gccggcgcac
     7741 gaaaaagaca ccttcatgag tctcccaaag cggttctttt cggaaacgtg acggaggatc
     7801 gggcggaaag ggatcgttta tggaatgaga ggtgggtact gcttgctcgg ctgcttagtg
     7861 ctctgcttgc tggattgctc aataaacggt ctttgcaacc aagcaaccaa gcaatcccgc
     7921 tccgaccccc gacccccgaa aacccatcct cgaattacac ttacgcgatg acgattgccg
     7981 gtgtgatcct cgcggc
//
LOCUS       DATLVW010000150         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_50731, whole genome shotgun sequence.
ACCESSION   DATLVW010000150 DATLVW010000000
VERSION     DATLVW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50731"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VNN25_04885"
     CDS             <1..328
                     /locus_tag="VNN25_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90896.1"
                     /translation="TGFDSATKGFLNTRGEQLSSGHASRPSSTSTQVPFRPFAPKEIA
                     AQHEVTQEAVQAAPQVGAEGEIYDPPFFRKGFSRPDGSGGFGPMASSDFGNDLDIPTV
                     IRNLSD"
     gene            complement(545..2038)
                     /locus_tag="VNN25_04890"
     CDS             complement(545..2038)
                     /locus_tag="VNN25_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90897.1"
                     /translation="MNPARSLSRFIVGSMLCLLLVPAIALAKSGNSADPLARMTASGS
                     VAIWTTAAGHDKIALTVVGPDGSVFQKEFAAGVTPMFRLQDLGGKATDGSYTYEMRIV
                     PNVSSSVRQQLAAAREAGDDVAAARIQREAGLAEQMVQSGTLSVSNGAFIDPDATEPV
                     LRQPAAAAPTSSGTVSASSITPPKKISPLDIVQADDVIIQGSLCVGLDCVNNESFGFD
                     TIRLKENNTRIKFDDTSTSTGFPNHDWQLTANDAASGGANKFSIEDITAATVPVTVTG
                     SAPTNSVFVDSTGRVGFRTATPVLDLHVATSNTPAMRLEQNNSGGFTAQTWDIAGNEA
                     NFFVRDVTGGSRLPFRIRPGAPTSSIDINASGNVGIGTASPSRSLEVDNSTIPALGLR
                     NTGQAADQKYWEFSVFGTQLRGSVQNDANTTEAFWLQVARGAGNAINSVSFPNGNVGV
                     GTTSPTSKLQVVGLSVFANNAAAVAGGLSAGAFYRTGGDPDLVCVVH"
     gene            complement(2191..>2724)
                     /locus_tag="VNN25_04895"
     CDS             complement(2191..>2724)
                     /locus_tag="VNN25_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH90898.1"
                     /translation="KGPSAVIKTHHNVGGLPEKMGLKLIEPVRELFKDEVRRLGLELG
                     LEDEFVWRHPFPGPGLAVRILGEVTAERVAILQNADEIVIEEIRRAGLYREISQAFAV
                     LLPVRSVGVMGDERTYENVVAVRAVSTTDFMTADWYRMPHDVLDAISRRIVNEVRGIN
                     RVVYDISSKPPSTIEWE"
BASE COUNT          498 a          812 c          890 g          524 t
ORIGIN      
        1 gaccggattc gattcggcga cgaagggttt cctcaacacg cggggcgagc agctttcgtc
       61 aggtcacgcg tcgcggcctt cgtcaacttc gacgcaggtg ccgttccggc cgttcgcgcc
      121 gaaggagatt gcggcgcagc acgaggtaac gcaggaagcg gtgcaggcgg cgccgcaggt
      181 aggcgcggag ggggagatct acgatcctcc cttttttcgc aaaggattca gccggcccga
      241 cggctccgga ggtttcgggc cgatggcgtc gagtgatttc ggcaatgacc tcgatattcc
      301 gacggtgatc cgcaacttga gcgactagcg tttcccacgc ggcgcgtccc tcctgcgcgc
      361 tgcacgcgat ccaggggaga gggacccggg atcatctgct ctgtaacggg gcgcggtctt
      421 ccgccgcgcc ccgtactttt ttctgggagt gccgagctcg atgatcgcgc gcaaagaaaa
      481 accgcgcggc gcgtggagga tctccacacg ccgcgcggct caggtcaagc gattcgcgag
      541 gggtctagtg aacgacgcaa acgagatcgg gatcgccgcc cgtccgatag aacgcgccgg
      601 ctgacaatcc gccagcgact gccgcggcat tgttggcgaa gaccgacagg ccaacgacct
      661 gcagtttgct ggtaggcgat gtcgtcccaa cgcccacgtt gccgttaggg aagctgacgc
      721 tattgatggc attgccagcg ccccgagcga cctgcaacca gaacgcctcg gtggtatttg
      781 cgtcattctg gacggaaccg cgcaactgcg ttccgaagac ggagaactcc cagtacttct
      841 ggtctgctgc ctgcccggtg tttcggagtc ccaacgccgg gatggtgctg ttgtctacct
      901 cgagactccg cgacgggctg gctgtgccaa taccgacgtt gccgctggcg ttgatgtcga
      961 tcgagctggt cggcgcgccg ggacggatgc ggaacgggag gcgcgagccg ccggtcacat
     1021 cgcgcacgaa gaagtttgct tcgttgcccg cgatgtccca ggtctgcgcg gtgaatccgc
     1081 cgctgttgtt ctgctcgagg cgcatcgccg gcgtgttgct ggtggcgaca tgaaggtcga
     1141 gcactggcgt ggcggtacgg aagccaacgc ggcccgtgct gtcgacgaag acggagttcg
     1201 tcggcgcgga gccggtgacc gtgaccggaa ccgtcgcggc ggtgatgtcc tcgatggaga
     1261 acttgtttgc gccgccgctg gcagcatcat tggccgtcaa ctgccagtcg tggttcggga
     1321 agccggtcga cgtgctggtg tcgtcgaact tgatgcgtgt gttgttttcc ttcagccgga
     1381 tcgtgtcgaa gccgaacgac tcgttgttca cgcagtcgag gccgacacaa aggcttccct
     1441 gaatgatcac gtcatcggcc tggacgatat cgagcggcga gatcttcttc ggcggggtga
     1501 tcgatgaggc gctcaccgta cccgaagagg tgggggccgc cgccgcaggc tggcgaagaa
     1561 ccggctcggt ggcgtccgga tcgatgaatg cgccgttgga caccgacagt gtccccgact
     1621 gcaccatctg ctcggccagt ccggcttcgc gctggatgcg ggccgccgca acatcgtcgc
     1681 cggcttcgcg cgccgctgcg agctgctgtc tgacggagga gctcacgttc gggacgatgc
     1741 gcatctcgta cgtgtaggag ccgtccgttg ccttgccgcc cagatcctgc agacggaaca
     1801 ttggcgtgac gcccgccgca aactctttct ggaatacgga tccgtcaggg ccgacgacgg
     1861 tgagcgcgat cttgtcgtgg ccggctgccg tcgtccagat tgctaccgaa cctgatgccg
     1921 tcatgcgggc aagcgggtcg gcgctattgc cggatttggc gagcgcgatc gcggggacta
     1981 gtaacagaca caacatggag ccgacgatga aacgggaaag agaccgggct ggattcatgc
     2041 aggttctcct cttcaaccac gcacgaaatg tgcgaggaac tgattttttg gtaggacgcg
     2101 tatgagtaac ttacaacgtc ttgtgacgca gagctacaca aaaatggaaa ctcgagggac
     2161 ggaacaaata aaatagattt ggaatctccc tcactcccat tcgatcgtgc tgggaggctt
     2221 cgaggagatg tcgtagacca cccggttgat gccccggacc tcgtttacga tgcgccggct
     2281 gatggcgtcg agcacgtcgt ggggcattcg gtaccagtcg gcggtcatga agtcggtcgt
     2341 cgatactgca cggaccgcga cgacattttc gtaggtccgc tcgtcgccca tcactcccac
     2401 gctacgcacc ggcagcagaa ccgcaaatgc ctgagaaatc tcacgataca ggccggcgcg
     2461 acggatctct tcgatcacga tttcatcggc gttctgaagg attgcgacgc gctcggcggt
     2521 tacttcgccg aggattcgca cggccagccc ggggccggga aacggatggc gccagacgaa
     2581 ttcgtcctcc agcccaagct cgagtccgag acggcgcact tcatccttga acagctcacg
     2641 aacgggctca atcagtttga gtcccatctt ctcgggcagc ccgccgacgt tgtggtgcgt
     2701 cttgatgacg gccgagggac cttt
//
LOCUS       DATLVW010000151         5661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_133508, whole genome shotgun sequence.
ACCESSION   DATLVW010000151 DATLVW010000000
VERSION     DATLVW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133508"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VNN25_04900"
     CDS             <1..340
                     /locus_tag="VNN25_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH90899.1"
                     /translation="RGFLLKDISYQQLMSAIRAIAAGGTVFQPAITQRLLRAAGTPAP
                     DAMPVEELTARELEVVRLMAGGYSNKEIAHALGTAEGTIKNQVSSILSKFGVRDRTRA
                     VLKALEARLL"
     gene            complement(376..969)
                     /locus_tag="VNN25_04905"
     CDS             complement(376..969)
                     /locus_tag="VNN25_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90900.1"
                     /translation="MKHTWISYTAALAIVFFAGTTSAMAQADLSLTFSGGPEFIGTVG
                     VGTGGPARPVAPGSVAEYGFLVFNSGPAAALGVVVTITFPVGITIAPLNSPGCSSSLV
                     AGNPTVTCSQASLASGGSYAGDIVVGLANNYPWQNGLTATASVTSSTPDPLSSNNSVT
                     DHLVVDPPVWIPAMDTRVLAALIAVLALVGVVSVSRT"
     gene            complement(966..2843)
                     /locus_tag="VNN25_04910"
     CDS             complement(966..2843)
                     /locus_tag="VNN25_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH90901.1"
                     /translation="MIGKALTHSITVIVGLKLDHEWVPEGYLAREDVSKQRAAIDTAL
                     ARLKTRYIDKMLDIQNFSPIPYACFVADVGLLQRLDHDDEVTSIELNELGSVGLRQSA
                     PLVGATAAWSAGYDGTGQTIAIVDTGIDKTHPFLSGKVVAEACSSNPEFGVSSSTVCP
                     NGQTQMVGTDAGVNCNVSISGCSHGTHVAGIAAGSSTTDGIWGIARGAKLISVQVFHR
                     VDDQSVCLSFGDSTPCAETSVRDIAAGLNQVADFAQQFQIAAVNISIQFQNFFSTREI
                     CRTSQPAVGTAIQNLRSRGIAVVGITGNYGNKSALAWPGCIPGVIDVGASTKDDAVAG
                     FSDSTSELDLLAPGDGNATHPGITSSVPGGGYAAYWGTSMAAPHVAGALAILRQKSPS
                     ASAGLLLSDLATTGVLLSDPQNQVTKPRINILAALSADHTPPSPPGSLVAAPTAPTSV
                     SLSWTASSDEHGIGQYSVQRRSFSTASFIEVGTSTSLSYADTSVAANSTYQYQIKAVD
                     TSGNMSAASNSVRATTILFSDDPLMVLTTSIRGVHVTELRSAVDSLRTFASLSAATWT
                     DSVPFGIIIKATHIQELRDRLGEALSALGITPPPYTDPSLSPGVSIKGIHVVEIRRNT
                     K"
     gene            3104..3265
                     /locus_tag="VNN25_04915"
                     /pseudo
     CDS             3104..3265
                     /locus_tag="VNN25_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002807809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(3380..4015)
                     /locus_tag="VNN25_04920"
     CDS             complement(3380..4015)
                     /locus_tag="VNN25_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90902.1"
                     /translation="MRKTNKSVVNPSNPVTAAPTAEDIAAQLMGLMDQIEALVPGFTP
                     HDTNDARRVANVARFAKDLIPQVITTVTALPSVGNVNIFDVEDGKTALELDASMRPVA
                     QRLASLLDGVTFTVGSRLAKSTGQGLAMFAWMKKHARTPEGVAARPYVDEMSRTMKRI
                     MNRRKPVHPPTPVPAGAQSLLAPNLAQAAATAPDDDLPEDFRKALEEAVKD"
     gene            complement(4281..4874)
                     /locus_tag="VNN25_04925"
     CDS             complement(4281..4874)
                     /locus_tag="VNN25_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90903.1"
                     /translation="MSKEIEIAVDTPPANGSMPDVDLLLALIDQIEANVPGFIPYDIN
                     DARRVGNLARFAKDMIPKMIATVSSLPSVAGMNTFDVVEGKISLAYDTAIQPVIQRLS
                     ALLNGVQFTTDSRLARSTEQVLATYAWMKKKAKSPSGVELRPYRDDMALTMKKVLNRR
                     SKPASKPAALEEAAEPVDDDDDRSEDSRTAPDDDVKN"
     gene            5171..5485
                     /locus_tag="VNN25_04930"
     CDS             5171..5485
                     /locus_tag="VNN25_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90904.1"
                     /translation="MRSDEPTMHIVNLTMASNESTLCIVNLTMSSDESTLGIVDMTIW
                     SDELTVCIVDMTLCIIEMTMSIVDSTMRIVDPTIRRPRLTMCINDSSMRLEDPSRLRV
                     FA"
     gene            complement(5538..>5661)
                     /gene="pheS"
                     /locus_tag="VNN25_04935"
     CDS             complement(5538..>5661)
                     /gene="pheS"
                     /locus_tag="VNN25_04935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401887.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXH90905.1"
                     /translation="PEEWSGFAFGLGLDRCAMIKFDIPNIRTLFENDLRVLQQF"
BASE COUNT         1182 a         1523 c         1788 g         1168 t
ORIGIN      
        1 gcgcggcttc ctgctcaagg acatctcgta tcaacaactg atgagcgcga tccgagccat
       61 tgccgcggga ggaacggtat ttcagccggc gatcacgcag cgtcttcttc gcgccgccgg
      121 aacgcccgct cccgacgcca tgccggtcga agagctgacc gcgcgcgagc tggaagtggt
      181 ccgcctcatg gccggcggct acagcaacaa agagatcgcc cacgccctcg gaaccgccga
      241 gggaacgatc aagaaccagg tctcgagcat cctgtcgaag ttcggcgtgc gcgatcggac
      301 gagggcggtg ctgaaggcgc tggaagcgcg gttgctgtag tcgcggaggt ttcttcgtcg
      361 agtggctacg aatacttacg tgcgagacac gctcaccacc ccgacgagcg ccagcacggc
      421 gatcaacgcc gcgagaacgc gtgtgtccat cgccggaatc catacaggag ggtcgacgac
      481 gagatggtcg gtcacgctgt tgttcgaaga gagcggatcg ggagtcgacg aggtaacgga
      541 tgcggtggcg gtcaaaccgt tttgccaggg atagttgttc gccagaccga ccacaatgtc
      601 tccggcgtac gagccgcctg acgcgaggct ggcttggctg catgtcaccg tgggattgcc
      661 agccacaagc gaggaagaac agccaggaga attcagcgga gcgatcgtta tccccaccgg
      721 aaacgtaatc gtgacaacga ctccgagcgc agcggccggg ccgctgttga atacaaggaa
      781 accgtattca gccaccgagc caggagcaac cggcctcgcc ggaccaccgg tgcccacacc
      841 gacagtaccg ataaactcgg gaccgccaga gaaggtcagc gaaaggtctg cttgtgccat
      901 agcactggtg gttcccgcga agaaaacgat cgccaatgca gcggtgtatg aaatccatgt
      961 gtgtttcatt tcgtgttcct ccggatctca acaacatgga tgcctttgat ggacacaccg
     1021 ggcgacaagc tcggatcggt gtacggcgga ggcgtaatgc cgagcgcgct gagggcttca
     1081 ccaaggcgat cccgaagctc ttggatgtga gtcgccttga tgatgatacc gaacggtact
     1141 gagtcggtcc atgtggcggc tgagagactt gcaaacgtcc tcaggctgtc tactgcactc
     1201 cgcagttccg tgacatgcac accgcggatt gatgtggtca gcaccatgag ggggtcgtcc
     1261 gagaagagaa tcgtggtcgc tcgaacgctg ttgcttgcag ccgacatgtt gccggatgtg
     1321 tcgacagcct tgatctggta ttggtaggta ctgttagcgg caaccgacgt gtcggcgtat
     1381 gacagtgagg tgctcgttcc gacctcaatg aaggatgctg tcgaaaagct gcggcgctgg
     1441 acactgtatt ggccgatgcc atgctcgtct gatgacgccg tccaggagag cgagaccgac
     1501 gtcggtgcgg tcggagcagc cacaagagaa cctggaggtg aaggcggagt gtgatcggca
     1561 ctgagtgctg cgaggatgtt gatgcgcggt tttgtgacct gattctgtgg atcggacagg
     1621 agcactccgg tcgtggcaag atcacttagg agcaggcctg cagaggctga gggcgacttt
     1681 tgtctgagaa ttgcgagcgc gcccgctaca tggggcgcag ccatcgaggt accccaatat
     1741 gctgcatatc cgccgcccgg cacagatgag gtaatcccag gatgggtggc gttcccatct
     1801 cccggtgcaa gaaggtcaag ctccgaagtg ctatccgaga aaccagctac agcgtcatct
     1861 ttggtagatg ctccgacgtc gatgacgcct ggaatgcagc caggccaagc cagtgcgctt
     1921 ttgttcccgt agttgccggt gattcctaca acagcgattc cccttgaccg cagattctgt
     1981 attgcagtgc cgacagctgg ctgagaagtt ctacagatct ctctcgtgct aaaaaaattc
     2041 tgaaattgga tgctgatatt cacggcggcg atttggaatt gttgcgcaaa atctgcaact
     2101 tggttaagac ctgcggcgat atcgcgtacc gaagtctcag cacaaggagt agaatcgccg
     2161 aacgagaggc atacgctctg gtcatctacg cggtggaaga cctggacgga aatcagtttc
     2221 gcgcctcggg cgatgcccca gataccgtct gtagttgacg agccggcggc gatgcccgcc
     2281 acgtgagttc cgtgagagca gccggagatc gagacattgc agtttacgcc cgcgtctgtg
     2341 ccgaccatct gcgtttggcc attggggcag accgtggacg aagagacgcc aaattctgga
     2401 ttggaggagc aagcctcggc gacgactttg cctgaaagga atggatgagt cttgtcaatg
     2461 cccgtgtcaa cgatggcgat cgtttgcccg gtgccgtcat agcctgctga ccatgcagcc
     2521 gtcgcaccca cgaggggagc gctctgccga agtcctacag agccgagctc gttgagctcg
     2581 atgctcgtga cttcatcatc gtggtcaagg cgttgcagca gcccgacatc ggctacgaag
     2641 catgcgtacg ggatcggcga aaaattttgg atgtcgagca tcttgtcgat gtatctcgtt
     2701 ttcagtctag ccagcgccgt gtcgatcgcg gctcgctgct tgctcacatc ctcacgagcg
     2761 aggtagccct cgggaaccca ctcgtggtca agcttcaacc cgacgatgac ggtaatcgag
     2821 tgagtcagcg ctttgccgat caatacgtcg atcggtgcgc ccagccggga tcccggtgac
     2881 ggtgtggcag gagcgctggc gtccggctgc acgaacaagt atctcgtgga gagacgagat
     2941 acttgcgccg aggcgatgaa ggccgttgag atgcaaacga caaacactgt tgcgcgcatc
     3001 gcgcaaagac gcaaatggac ctccgggaaa cgcgacgcaa tgtgcggcga ggtgcagact
     3061 agtcgatcaa ggcaatgacg tccagcttga cgtaagcaca ttttcgcggc gcgaactgga
     3121 agtcgtcccc ctaatggccg gcggttacag caacaaagaa atcgcccacg ccctcggcac
     3181 cgccgaggga acgatcaaga atcaggtctc gagcatcctg tcgaagttcg gcgtacgcga
     3241 tcgaacgaag gcggtattga aagcgctcag aagcgcggtt gctgtaattc gtggtctcgc
     3301 gatgtgttca atccgcgcgc ttacccaata ccaaaacacc gccccccgcg cgagactcgc
     3361 gcgaggggcg gggcggggtc tagtccttca cggcctcttc gagggccttg cggaaatctt
     3421 ccggaaggtc gtcgtcgggg gctgttgcgg cggcttgggc gagattcggc gcgagaaggc
     3481 tttgcgcgcc tgccggcacc ggcgtcggtg gatggactgg tttacggcgg ttcatgatcc
     3541 gcttcatcgt tcgtgacatt tcatccacat acggacgcgc tgccaccccc tcgggggttc
     3601 tcgcgtgttt cttcatccag gcaaacatcg ccagtccctg tccggtggat ttcgcgagcc
     3661 ggctgccgac ggtgaatgtg acgccgtcga gcagcgatgc cagacgctgc gcgaccggtc
     3721 gcatggaggc atcgagctcc agagcagttt tcccgtcctc gacgtcgaag atgtttacgt
     3781 ttccgacgga cgggagagcg gtgacggtgg tgatgacctg tgggatgaga tccttggcaa
     3841 agcgcgccac gttggctacg cgcctggcgt cattcgtatc gtgcggcgtg aagccgggga
     3901 cgagagcttc gatctgatcc atcaggccca tgagctgggc ggcgatgtcc tcggccgttg
     3961 gtgcagcagt gactgggttg gaggggttga caacactctt gttcgttttt cgcatgatca
     4021 ctcctcgaaa ccacgcgaga cagactctct ccgtataccg cgtggggtta ggggtttagt
     4081 caattcgagc cagctctgta cgccggcagc gacgcgctgg cggcgaggtt catcgatgag
     4141 acctgtcttc ttagacgagt ctcggtcggg caaggttacg gagggacaag tttctggggc
     4201 tgatctgatg agggtgtggg gagtcggggg tggggagtgg gagcgtatcg acgaccccta
     4261 accccgagct gaaataggag ctaatttttc acatcgtcgt ccggcgctgt gcgggaatcc
     4321 tcggaacgat cgtcatcatc gtcgacgggt tcggccgctt cttccagtgc tgccggcttc
     4381 gatgctggtt tgctgcggcg gttcaggacc ttcttcatcg tcagggccat gtcatcgcga
     4441 tacggacgaa gctcgacacc agagggactt ttggcttttt tcttcatcca ggcgtaggtc
     4501 gccaacacct gttcggtgga tctggcaaga cggctgtcgg tcgtgaactg aacgccgttg
     4561 agcaacgccg acaggcgttg gatgaccggc tggatggcgg tgtcgtacgc cagggatatc
     4621 ttcccctcga cgacgtcgaa ggtgttcatt ccggcgactg agggaagaga ggataccgtg
     4681 gcgatcatct ttggaatcat gtctttggca aagcgggcca ggttgcctac gcgtctggcg
     4741 tcgttgatgt cgtagggaat gaacccgggg acgttcgctt cgatctgatc gatcagggcc
     4801 agcaacagat cgacatccgg catggatcca ttggcaggcg gtgtatcgac ggcgatctct
     4861 atctctttgc tcataagatc ctcgaagaat cacgctggtc cgacatgttg tgacccgcgt
     4921 gggtaaacgg gttgaagtac ttcaagccaa ctcgacgtct cgtcagcgac gcgctgccga
     4981 gggaggtcat cgatagacct attcggttag acggggtcac ctgacaaaag gttccggctg
     5041 tgcaagagaa ggtcgttgag cgcgcggaat aatcgcgatt ttctggtgat tcatgccgcg
     5101 tttgggactc attggtccag ttcttgtgaa ttggatcatc gagtcttcta gccgcattgt
     5161 cgatttgacg atgcgaagcg atgaaccgac gatgcacatc gtcaatctga cgatggcgag
     5221 caatgaatcg acgctgtgca tcgtcaatct gacgatgtcg agcgatgaat cgacactagg
     5281 catcgtcgat atgacgatat ggagcgatga attgacggta tgcatcgtcg atatgacgct
     5341 gtgcatcatc gaaatgacga tgtcgatcgt ggattcgaca atgcgcattg tcgatccgac
     5401 gatacgcaga ccacgattga cgatgtgcat caatgattct tcgatgcgtc tagaagatcc
     5461 ttcgcgactt cgcgtcttcg cgtgaacagg gaatcacgcg aagacgcgaa gacacgaagg
     5521 agaaacgaaa ggcgggctca gaactgctgc agtacgcgca gatcgttctc gaagagcgtg
     5581 cggatgttcg ggatgtcgaa cttgatcatg gcgcagcggt cgaggccgag gccgaaggcg
     5641 aagccgctcc actcctcggg g
//
LOCUS       DATLVW010000152         3261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_542930, whole genome shotgun sequence.
ACCESSION   DATLVW010000152 DATLVW010000000
VERSION     DATLVW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542930"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..653)
                     /locus_tag="VNN25_04940"
     CDS             complement(129..653)
                     /locus_tag="VNN25_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90906.1"
                     /translation="MKISSMVLLAIAISSTAISGAEQGNVTGKMVINGKTIKLTHVYA
                     SAGPSRWDKKKVEVRVILSDVPLSDEELASGSKREALANAGKLHAMELILADDPMGHP
                     GKRSMSNDIYDSAFTTAQQPMRLSGLDTFETKTDDGKTIAGHHFMESPHKFSDIGNNV
                     TFQDDVTFTAAVAK"
     gene            complement(1492..2649)
                     /locus_tag="VNN25_04945"
     CDS             complement(1492..2649)
                     /locus_tag="VNN25_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH90907.1"
                     /translation="MRPIADSFALVLTGGGARAAYQVGVLRCLARHLPKNAFDIITGV
                     SAGAINAAFVASRNAPLPAVIDELSEVWRTLELKDVIRIDSRSLARNVFGWGTKLISG
                     GNRVAPGIRGLVDTTPLRHLLERLFVTNDKGEISGIAENIVRCHPRAVSVTALDYTTG
                     KTVTWTTGCDIETWSRPLRRSVEQPLSVDHIMASAALPLVFPAVLVGKHWYGDGGIRL
                     SAPLSPALHLGATRMLAISTHYAPSSSEADEPQIAGYPPPAQILGQLMDAIFLDVMDE
                     DTVRLQRSNEFLGHLPPDLRGGYRPVDFAVIRPSQDIGKLAAEYEPALPRPFRFLTRS
                     LGTRETSNADFMSLFMFVPEYLQRLMEIGEADADANIEAIMRVGTGTVQAR"
     gene            complement(2686..3186)
                     /locus_tag="VNN25_04950"
     CDS             complement(2686..3186)
                     /locus_tag="VNN25_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HXH90908.1"
                     /translation="MEKILVSACLLGQPVRHNGAHKRSDDQILQRWIEEGRVVAVCPE
                     IAGGLPVPRPASEVVGGADGSKVLAGEVKVIDFNGRDVSHHFVSGASVALEAARANNI
                     RIAILKEGSPSCGSGYTYDGTFTSTRVPGHHGVTAALLRDNGVRVFSETQLAEAEECL
                     LQLGSK"
BASE COUNT          600 a          984 c         1050 g          627 t
ORIGIN      
        1 gagaagtaac gtcttcttcg cgtcttcgcg tgagcatctg tgtcacgcga agccgcgaag
       61 ggcgcgaagg agaaaagcgc tcgatcgcga agagatctac cgcccttctc cagcgtccct
      121 cgcgtcgcct acttcgcaac cgccgccgta aacgtcacgt cgtcctgaaa cgtgacgttg
      181 ttgccgatgt cgctgaattt gtggggtgat tccatgaagt ggtggccggc gatggtcttg
      241 ccgtcgtcgg tcttggtctc gaacgtgtcg aggccgctca ggcgcatcgg ctgctgggcg
      301 gtggtgaagg cggaatcgta gatgtcgttc gacatcgagc gcttgcctgg atgccccatc
      361 ggatcatcgg ccaggatcag ctccatcgcg tgtagtttgc cggcgttcgc cagcgcctcg
      421 cgtttgctgc cgctggcgag ttcctcgtcg ctcagcggca cgtcggagag gatcacgcgg
      481 acctccacct tcttcttatc ccaacggctc gggcctgcgg acgcgtagac gtgcgtgagc
      541 ttgatggtct tgccgttgat caccatcttt ccggttacgt ttccctgctc ggcgccagag
      601 atggcggttg atgagatagc gatggcgagg agcaccatgg acgagatctt catagggtca
      661 ttctatgctt cgggcgagag ggtcagctcg agaccgggga tgcgcacgta gcgcaggcat
      721 tcctgcctgc taccgtcggg cgtccctgcc cggcgggggt tgccgcgaaa tgcgaaattc
      781 accgccgctg cttcgacatc gaaagcctcc cctcgatatt ggagacgctc cgtttgacat
      841 tagcggcgct ccgtttgaca ttggccgcgc cccgtttgac attagccgcg ctccgtttga
      901 cattagccgc gccccgtttg acattagccg cgcgctgttt gacattagcc gcgctccgtt
      961 tgacattagc cgcgctccgt ttgacattag cggcgctccg tttcgaacga cgaggcagta
     1021 gggtcagacc ttcgttcgtc gtctgcctgc aaaccattga aaaagctatt acttgcgagg
     1081 gcacaccggg agcgctgcga tgcctcttca gttattcgca ctaaattcag gagaagaaaa
     1141 agaagacgtt tcacgcggag tcgcggagat gcggaggaga gacgccgcgg cctccgcgtc
     1201 tccgcgtgaa aaacaggtgc tcccccagca ccacgcaaat aacaaccgaa ggcatgaggg
     1261 tcagaccttc gctcgttgcc tgcctgcaaa tcattgaaaa gcggatactt gcgagggcac
     1321 cttgagccgt cacgatgttt tcttttgtta ttcgcatcta ttcgggcgaa aaaacgaagg
     1381 cctggcccca cggcaacgcc acagcaaccg gcgacccact cagttgcgcg actcacggaa
     1441 accaccgccg ggcgaggacg cccgacggcc gcaggagacg cctgcattcc tctagcgcgc
     1501 ctgaaccgtc cccgtcccta ccctcatgat cgcctcgata ttcgcgtcgg cgtcggcttc
     1561 gccgatctcc atcaggcgtt ggaggtattc ggggacgaac atgaagaggc tcatgaaatc
     1621 ggcgttggag gtttcgcggg tgccgaggct gcgggtgagg aagcggaagg ggcgtgggag
     1681 ggcgggctcg tattcggcgg ccagcttacc gatgtcctgc gacgggcgga tgacggcgaa
     1741 atcaaccggc cggtaacctc cccgaaggtc gggcgggagg tgacccagaa actcgttgga
     1801 gcgctgcaga cgcacggtgt cctcgtccat cacgtcgagg aagatcgcgt ccatcagttg
     1861 gccgaggatc tgggccggag gcgggtagcc ggcgatctgc ggctcgtcgg cttcggatga
     1921 cgagggcgcg tagtgcgtgg agatggccag catgcgcgtg gcgccgaggt gcagggctgg
     1981 agagagcggc gcggagagac ggatgccgcc atcaccgtac caatgctttc ccacaaggac
     2041 cgcgggaaag acgagcggca gggcggccga ggccatgatg tgatcgacgc tcagcggctg
     2101 ctccacgctg cggcgcaggg ggcgcgacca cgtctcgatg tcgcagccgg tggtccacgt
     2161 cacggtcttg ccggttgtgt aatcgagcgc ggtcaccgac acggcccgcg gatgacagcg
     2221 gacgatgttc tcggcgatgc cggaaatctc gcccttgtcg ttcgtgacaa agagccgctc
     2281 cagcagatga cgaagaggcg tcgtatccac cagtccccgg atgcccggag cgacccggtt
     2341 tccgcccgaa atgagcttcg tcccccagcc gaagacgttg cgcgcgagcg agcgggaatc
     2401 gatccggatc acgtctttca actccagcgt tctccagacc tcggacagct catcgatgac
     2461 ggcaggcagc ggcgcgtttc gcgaggcgac gaaggccgcg ttgatcgcgc ccgcggaaac
     2521 gccggtgatg atgtcgaagg cgttcttcgg cagatgccgc gcgagacaac gcagcacgcc
     2581 cacctgatac gcagcgcgcg cgccgccgcc agtgagcacg agtgcgaagg aatcggcgat
     2641 cggcctcatg cacctaaaga atacgccgca actttccgat tacgatcact tcgatccgag
     2701 ctgcagtagg cactcttccg cttctgcgag ttgcgtttcg ctgaaaacgc ggacgccgtt
     2761 gtcgcggagc agcgcggctg tgacgccgtg gtgaccaggc acgcgtgtgg aggtgaacgt
     2821 tccgtcgtag gtgtagccgc tgccgcacga tgggctgcct tctttgagaa ttgcgatgcg
     2881 gatgttgttg gcgcgcgcgg cttcgagcgc gacgctggct ccgctcacga agtgatgcga
     2941 gacgtcgcgg ccattgaagt cgatcacctt gacttcgccg gcgaggactt tcgaaccgtc
     3001 cgcgcctccc acgacctctg aagccggccg cggcacaggg aggccgccag cgatctccgg
     3061 gcagacagcg acgacgcgtc cttcctcgat ccagcgctgc aggatctgat cgtcggaccg
     3121 cttgtgcgcg ccgttgtggc ggacgggctg gccgaggagg caggcgctga cgaggatctt
     3181 ttccattggc gttttacact taaccacaga gacacagagg acacagagtt gaagacacta
     3241 tctctgtgac ctctgtgtct c
//
LOCUS       DATLVW010000153         6844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_253314, whole genome shotgun sequence.
ACCESSION   DATLVW010000153 DATLVW010000000
VERSION     DATLVW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253314"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VNN25_04955"
     CDS             complement(<1..373)
                     /locus_tag="VNN25_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH90909.1"
                     /translation="MLSRDARLLFAARIVRLFAYGFVAVVLVLYLSSIGLDDHRIGLL
                     LSLTLAGDVIVSLYLTTRADSLGRRRTLLAGAVLMIGAAVVFALTRDFTLLVLAATIG
                     VISPSGNEVGPFLAVEQVALAH"
     gene            complement(378..1652)
                     /gene="tig"
                     /locus_tag="VNN25_04960"
     CDS             complement(378..1652)
                     /gene="tig"
                     /locus_tag="VNN25_04960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXH90910.1"
                     /translation="MLLNYEDVSPVKKIVEVEIPAGLISAESQRVTSEFSRQVKIPGF
                     RPGKVPLGVIRSRFAKEIQEQVMERLLPQTFSEAIADKGLEVVGDPHLQHVDPVIEGA
                     PVKYKAEFEVKPQFELKQYRGLVIDDPKIEVAEADVDGMIERLREQASVYRPETERGL
                     EENDFAMIEMTTSGEGIDAETRGGHFRLGEETPLPELHEALRGKKPGESATFDKTYPD
                     DAQNESFRGKNIHHEVTLKEIRVQEKPEITDEFAQSTGGWENVAQMREAIAADIRKHR
                     ELEVRRLKQTQLSELLLAAHELEVPDTLAEEEVGRSLQNYARFLVSQGVDVQKAEIDW
                     RKMRDEFLPEAVKRVKRSLILEQIAKKENLIVSDVEADAHIRRAAKEADRDFAEVKHQ
                     LRHDGGYEKLRTSMSQDKALDLVLDEATLRSA"
     gene            complement(1688..1773)
                     /locus_tag="VNN25_04965"
     tRNA            complement(1688..1773)
                     /locus_tag="VNN25_04965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Leu,seq:tag)
     gene            complement(1828..1903)
                     /locus_tag="VNN25_04970"
     tRNA            complement(1828..1903)
                     /locus_tag="VNN25_04970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1868..1870),aa:Lys,seq:ctt)
     gene            complement(1985..2539)
                     /locus_tag="VNN25_04975"
     CDS             complement(1985..2539)
                     /locus_tag="VNN25_04975"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXH90911.1"
                     /translation="MLTVPNALTLLRLFAVPVFLYASFRGEYRLAFFIFAGAAFTDFL
                     DGVIARRFNQRSRIGAILDPAADKTMMVGGYLFYTLRDGLPCGIPGWLTFTVLIRDFV
                     IILFAYLLYTRIHVKRFPPSWAGKTSTMLQAGTLAVTIEINAFGPALFPFLQVLFRIA
                     MLATLFSAWDYLRKGKGMIASANP"
     gene            complement(2650..3333)
                     /locus_tag="VNN25_04980"
     CDS             complement(2650..3333)
                     /locus_tag="VNN25_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH90912.1"
                     /translation="MLPTSFYRKFSLFAELDDRELASIAAVAKTRRYAKDDAVFHADE
                     IGDVFYLIREGQVKVTMISPEGKEIILSILGVGDFFGEMALFDNEPRSATVIATEPLE
                     IVSIWRSDFLHILSENFSIAQKVLAELSKRLRDASNRIESLATMDVYGRLARFFLDLA
                     RQNGKSLDNDYIAVTRPTHQAIANMIGTSRETVSRLIHDLMKQNLLLSEGKTIYLRKS
                     ALDQFRAEL"
     gene            3375..4697
                     /locus_tag="VNN25_04985"
     CDS             3375..4697
                     /locus_tag="VNN25_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH90913.1"
                     /translation="MLHSVAKFPTRVAIKRIIPISSGKGGVGKTTFAINFSLALSRHG
                     RTILVDLDTGTSAIRNAIDVPIDKDLYHFFRKSEPLNSCVTHLDAKLDPKGVFRNFGF
                     VAGPLHLIDEITNFGAANKDRLIEAINGLDADYVILDMRAGLDANVIDFLPFSNSGVL
                     IFTPHLPSATLAASDIVKAILFRKLRLIFSRESPFFSTVNGKLDFWKLINDLIDQVED
                     VYDSAIGNLDAFADDLAGNLGDHPITRTVQQTLEEFRVHYVLNLFNGVDDSFQTAVKP
                     FIENLTSNVSARCGVTNLGWITRSEAIHRANCNKIPAMLLPPEGKKAPKSNRVDQELE
                     DLRSMAIGMEPVRKKAARQQKSSTSVDPADHLANQLNVLRSMFDQKKDDDFHANFDYA
                     SQRALFTMKTRRPSEFGDIRVYRPAEMLATIFAKSNVPTSIDWGELDL"
     gene            4780..5634
                     /locus_tag="VNN25_04990"
     CDS             4780..5634
                     /locus_tag="VNN25_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH90914.1"
                     /translation="MPDPVQPDRAEMLDADEHTLYWELFGTGEREVVCLLNGLAMHTA
                     AWHSFLPRLRPDYDVLLWDYPGQGRSTSFDVPYFVDRIASYLVRILDRIGVERVHLVG
                     ISYGGLVALEFARQFQDRLHTLVVSGILLSREKLFDMYQDLSLRFYAGGPETFDLYTR
                     YMYEKIFGETFVRAAADRLESMRTNFYERYKDRTAALARLTLAQDPFFEALETRLDEY
                     RAIRTPALVIAAEHDRVIAPGVQRKIAAILPNSRFELVPDSGHVVYLEHPDRFFPMVR
                     QLFASKSV"
     gene            5812..6531
                     /locus_tag="VNN25_04995"
     CDS             5812..6531
                     /locus_tag="VNN25_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH90915.1"
                     /translation="MNLKDKVVIVTGGGAGIGQATAAAFAHEGAKVVAWDITSSPKVD
                     VTSRDSVESAAAMVIADHGRIDVLVNNAGIVRDAQLVKWKDGEVVSTMDDATFDAVVN
                     VNLKGVFLCTRAVVPHMIRQGHGVVLNASSIVGLYGNFGQTNYAATKAGVISFTQTWA
                     RELGRFGIRVNAVAPGFIATDILKAMPQKVLDSMAARTPLGRMGDPLDIANAYVWLAS
                     DAARFVSGAVLSVDGGLVLGT"
     gene            6553..>6844
                     /locus_tag="VNN25_05000"
     CDS             6553..>6844
                     /locus_tag="VNN25_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HXH90916.1"
                     /translation="MNYCNIVGTGSFLPEIEVTNDDLRERLSATAPDFVDKMEMASGI
                     LKRWRAPEGWAASDLAQRAATEALLRAEKVATDVDLIILGTDSPDYLTPATSV"
BASE COUNT         1347 a         2133 c         2040 g         1324 t
ORIGIN      
        1 catgcgccag cgcaacctgc tcgacagcca gaaagggccc cacttcgtta ccgctcgggc
       61 tgatcacgcc gatggtcgca gccagcacga gcagcgtgaa atcgcgtgtc agtgcaaata
      121 cgaccgcggc gccgatcatc agcacggcgc cggcgagcag cgttcgccgc cggcccaggg
      181 agtcggcccg cgtcgtcagg tagagggaaa cgatgacatc gccggcgagg gtgagcgaca
      241 gaagcaaccc gatccgatgg tcgtcgagcc cgatcgacga aagatagagc acgagaacga
      301 cggcgacgaa gccgtacgcg aaaagccgca cgatgcgcgc ggcgaaaagg agtcgcgcat
      361 cacgcgagag catgagatca ggcgctgcgc agcgtcgcct cgtccaggac gagatcgagc
      421 gctttgtcct ggctcatcga cgtccgcaac ttctcgtagc cgccgtcatg ccgcagctgg
      481 tgcttgacct ctgcgaagtc gcggtcggcc tctttcgcgg cacggcggat gtgcgcgtcc
      541 gcttcaacgt cgctgacgat cagattctct ttcttcgcaa tctgctcgag gatgagtgag
      601 cgtttgacgc gtttcacggc ctcgggaaga aactcgtcgc gcatcttgcg ccagtcgatc
      661 tccgctttct ggacgtcgac gccttgcgaa accaggaagc gcgcgtagtt ctgcagcgac
      721 cggcccacct cttcctcagc cagcgtgtcc gggacttcca gctcatgggc ggcgagaagg
      781 agctcggaga gctgcgtctg cttcagccgg cgcacttcga gctcgcgatg tttgcggatg
      841 tcggcggcga tggcctcgcg catctgcgca acattttccc atccgccggt gctctgggcg
      901 aattcgtccg tgatctctgg cttttcctgg acccggattt ctttgagcgt gacctcgtgg
      961 tggatgttct tgccgcgaaa gctctcgttc tgcgcatcgt cgggatacgt cttgtcgaac
     1021 gtggccgatt cgcccggctt cttgccgcgc agcgcttcgt gcaactccgg cagcggcgtc
     1081 tcttcgccga gccggaaatg gcctccgcgc gtttcggcat cgatgccttc gccggacgtc
     1141 gtcatctcga tcatcgcgaa atcgttctct tcgagtccgc gctcggtctc ggggcgatag
     1201 acgctggcct gctcgcgaag cctctcgatc atgccgtcga catcggcttc ggcgacttcg
     1261 atcttcgggt cgtcaatgac caggccgcga tactgcttca actcgaattg cggcttgacc
     1321 tcgaactcgg ccttgtactt gaccggcgct ccttcgatga ccggatcgac gtgctggaga
     1381 tgcgggtcgc cgacgacctc gagacctttg tccgcgatgg cttcgctgaa cgtttgcggc
     1441 aggagccgct ccatgacctg ctcctggatc tctttggcaa agcgcgaacg gatgacgccc
     1501 aggggtactt tgccgggacg gaagccgggg attttcacct gacggctgaa ctccgacgta
     1561 acgcgctgcg actccgcgga gatgaggccc gccgggatct cgacctcgac gatcttcttg
     1621 actggggaaa cgtcttcgta attgagaagc attcgctggc aaccctcgga tgaattttcg
     1681 gctggattgg tgcgaaaggc gggactcgaa cccgcacggc tttgggccac tggatcctaa
     1741 gtccagcgcg tctgccagtt ccgccacttt cgcccggatg caaaaaagga ccgagcgcga
     1801 gcccagtcct ctgcatcgta cttgatttgg tgcccccact aggactcgaa cctagaacca
     1861 acagattaag agtctgctgc tctaccaatt gagctatgga ggcttaggac ccctgctaac
     1921 ttcgcgcgcc caccatctat ttctcggcgc gagccgcgca ttctaggaga cgggccggaa
     1981 gcggtcaagg attcgcggac gcaatcatcc ccttcccttt tcgcaggtag tcccatgcgg
     2041 agaagagtgt cgcgagcatg gcgatgcgga agaggacctg aagaaacggg aacagcgccg
     2101 ggccgaacgc gttgatctcg atcgtcacgg ccagcgtacc tgcctgcaac atcgtcgacg
     2161 tcttcccggc ccacgacggc ggaaagcgct tcacgtgaat ccgcgtgtag agaaggtagg
     2221 cgaagaggat gatcacgaag tcgcggatga gcaccgtaaa cgtgagccat cccggaatcc
     2281 cgcacggcaa accatcgcgc agcgtgtaga aaaggtagcc accgaccatc atcgttttgt
     2341 ccgcggccgg atcgaggatc gcaccgatgc gcgagcgctg attgaaccgc cgcgcgatca
     2401 cgccatcgag aaagtcggta aacgccgccc ccgcgaaaat aaagaacgcc agccggtact
     2461 cgcctcggaa tgaggcatag agaaaaaccg gcaccgcgaa gagccggaga agcgtgagcg
     2521 cgttggggac ggtgagcatt gcggtagcgg agggtacgcg gttacgcggt ctcgcggaag
     2581 attagtctcg cgcactcgcg gttgtgatgc aggcacttgc gcgttgcacg agacttcgcg
     2641 aaccgagaat cacagctccg cgcggaactg atcgagcgct gatttgcgga gatagatcgt
     2701 cttcccctcg gatagaagga gattttgctt catcagatcg tggatcaatc gcgacaccgt
     2761 ctcgcgcgac gtgccgatca tgtttgcgat cgcctggtgc gtggggcggg tcacggcgat
     2821 gtagtcgttg tcgagtgact tgccgttctg acgggcgaga tcgagaaaga agcgagcgag
     2881 gcggccgtag acatccatcg tcgccagtga ctcgatccga ttcgaagcgt cccgaagccg
     2941 tttggagagc tccgccagca ctttctgagc gatcgagaaa ttctcgctga ggatgtgaag
     3001 aaagtcggag cgccagatcg acacgatctc gagcggctcg gtggcgatca ccgtggccga
     3061 gcgcggttcg ttatcgaaca acgccatctc gccgaagaaa tcgccgacgc cgaggatcga
     3121 gaggatgatc tctttcccct cgggtgagat catcgtcact tttacctgcc cttcgcggat
     3181 gagatagaag acgtcgccga tctcgtcagc atggaaaaca gcatcgtcct tcgcgtagcg
     3241 gcgcgttttc gccaccgctg cgatcgacgc cagctcacgg tcgtccagct ccgcgaagag
     3301 cgagaacttc ctgtagaacg atgttgggag cacttgcgcc gggattttag cagcaaagca
     3361 gtccagcaaa cccgatgcta cactccgtcg cgaagttccc aacgagagtg gccatcaaac
     3421 ggatcattcc gatctcctcc ggcaagggcg gagttggcaa gacaaccttc gccatcaact
     3481 tttcgcttgc cctctcgcgt cacggccgca cgatcctcgt cgacctggac acgggcacgt
     3541 cagcgatccg caacgccatc gacgttccga tcgacaaaga cctctaccat ttcttccgca
     3601 agagcgagcc gctcaattcc tgcgtgacgc atctcgacgc gaagctcgat cccaaaggcg
     3661 tcttccgcaa cttcggcttc gtggccggac cgctccacct gatcgacgag attacgaatt
     3721 tcggcgcggc gaataaagac cggctgatcg aagccatcaa cgggctcgat gccgactacg
     3781 tcatcctcga catgcgcgcc ggcctcgacg ccaacgtcat tgacttcctg ccgttctcga
     3841 actccggagt gctcatcttc acgccgcatc tcccgagcgc cacgctggcc gcgtccgaca
     3901 tcgtcaaagc gattctcttc cggaagctgc gcctgatctt cagccgtgag tcgccgttct
     3961 tttctaccgt caatggaaaa ctcgacttct ggaagctgat caacgatctc atcgatcagg
     4021 tcgaggacgt ttacgactcg gcaatcggaa acctcgacgc gttcgccgac gatctcgccg
     4081 gcaacctcgg cgaccatccc atcacccgca ccgtccagca gacgctcgag gagttccgcg
     4141 tccactacgt cctcaatctc ttcaacggcg tcgatgattc gttccagaca gccgtgaagc
     4201 cgttcatcga gaatctgacg tccaacgttt ccgcgcgatg cggcgtcacc aacctcggct
     4261 ggatcacgcg cagcgaagcc atccaccgcg ccaactgcaa caagatcccg gccatgctcc
     4321 tgccacccga ggggaagaaa gcgccgaaga gcaaccgcgt cgatcaggag ctcgaagacc
     4381 tccgctcgat ggccatcggc atggagcctg tccgcaagaa agccgcgcgg cagcagaagt
     4441 cgtcaacgag cgtcgatccc gccgaccatc tcgccaatca gctcaacgtc ctgcggtcga
     4501 tgttcgatca gaaaaaggac gatgacttcc acgccaactt cgattacgcc tcgcaacgcg
     4561 cgctcttcac gatgaagacg cgccgcccca gcgagttcgg cgacatccgc gtctaccgcc
     4621 ccgccgagat gctggccacg atcttcgcga agtcgaacgt gccgacctcg atcgattggg
     4681 gcgagttgga tttgtagcgt cgttggcgac cgtccctcac gctgcgcgat ctcgaaccca
     4741 cgcaatcctc agctccgagt gaatttcaaa ggaaggacga tgcctgatcc cgtacaacct
     4801 gaccgcgcgg agatgctcga cgcggacgag cacacgctct actgggagct tttcggcaca
     4861 ggcgaacgcg aggtcgtctg tctgctgaat ggactggcga tgcacacggc ggcgtggcac
     4921 agcttccttc cgaggcttcg cccggactac gacgtgctgc tatgggatta cccgggacag
     4981 ggccggtcga cgtcattcga tgtgccgtat ttcgtcgacc gcatcgcgtc gtacctcgtg
     5041 cgcattctcg atcgcatcgg tgtggagcgc gtacatctcg tcgggatctc gtacggcggg
     5101 ttggtcgcgc tggaattcgc tcgtcagttt caggaccggc tgcacacgct cgtagtgtcg
     5161 ggcattctcc tgtcgcggga gaagcttttc gacatgtacc aggatctatc gctccgcttc
     5221 tatgctggcg gtccggagac gttcgacctc tacacacgct acatgtacga gaagattttc
     5281 ggcgagacct tcgtccgcgc tgccgcggac cgtttggagt cgatgcggac gaatttctac
     5341 gagcgataca aggatcgaac ggccgctctg gcccggctga cactcgcgca ggatccattc
     5401 ttcgaggccc tggagacgcg gctggacgag tatcgcgcga tccggacgcc ggcgctcgtc
     5461 atcgcggcgg agcacgaccg cgtcatcgca cccggagtgc agcggaagat agcggctatt
     5521 ctgccgaaca gtcgctttga gctcgtgccg gactcgggtc atgtcgtcta cctcgagcat
     5581 ccggaccgct tcttcccgat ggtccgccag ctctttgctt cgaaatcggt ctaagggagc
     5641 ccacgctcgg ggaaggtgaa gtagccgggt ggagaatctt gacatccacc cccaaccacc
     5701 cataattgca tttacaaacc gaccggtcgg ctagtttcaa ccataggacc ctccattccc
     5761 gggaactcgc ggcgatctca gccgtgtccc tgtcaaaggc cgagcatgca actgaatctg
     5821 aaggacaaag tggtgatcgt caccggtggc ggcgctggga tcggccaggc aaccgcagcg
     5881 gcgttcgctc acgaaggggc gaaggtcgtc gcctgggaca tcaccagctc acccaaggtc
     5941 gacgtaacaa gccgggactc cgtcgaatcg gctgccgcga tggtgattgc ggatcacggg
     6001 cgaatcgatg tgctggtcaa taacgccggc atcgtccgcg acgcccaact cgtgaagtgg
     6061 aaggatggcg aggtcgtctc tacgatggac gatgcgacct tcgacgcggt cgtcaacgtg
     6121 aacctcaaag gagtgttcct ctgcacgcga gcggtagtgc cccatatgat ccgtcagggt
     6181 catggcgtgg tcctcaacgc ctcctcgatc gtaggcctct atggcaactt cggacagaca
     6241 aactacgcgg ccacgaaggc gggcgtgatc agctttacgc agacttgggc acgcgagctg
     6301 ggccggttcg gcatccgcgt gaatgcggta gctcccggat tcatcgccac tgacattctc
     6361 aaggccatgc cgcagaaggt cctggactcg atggcggcgc gaacgcctct cggtcgcatg
     6421 ggcgacccct tggacattgc gaatgcctac gtctggctgg cctcggatgc ggctcgattc
     6481 gtcagcggag cggtgctgtc agtggatggg gggttggtgt tggggacctg agggttcctg
     6541 actcctcttg tgatgaacta ctgcaacatc gtcggcacgg ggagtttcct tcccgaaatc
     6601 gaagtcacga acgacgacct ccgtgagcgt ttatccgcga ctgcgccgga cttcgtcgac
     6661 aagatggaga tggccagcgg aattctcaaa cgctggcggg caccggaagg atgggcggcc
     6721 tcggatctcg ctcaacgggc cgcgactgag gctcttctgc gcgcggagaa ggttgcaacc
     6781 gacgtcgacc tcatcatcct cggcacggac tcacccgact acctcactcc tgcgacctcg
     6841 gtcg
//
LOCUS       DATLVW010000154         4274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_133604, whole genome shotgun sequence.
ACCESSION   DATLVW010000154 DATLVW010000000
VERSION     DATLVW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133604"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..552
                     /locus_tag="VNN25_05005"
     CDS             70..552
                     /locus_tag="VNN25_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH90917.1"
                     /translation="MAFEIREATESDVPLILQFIRDLAEYERLAHMVVATEEQLRSTL
                     FGAVRVAEVLIGSSDGVPAGFAIFFHNYSTFLAQPGIYLEDLFVKPEFRGRGYGKALL
                     VRLAQIARDRNCGRVEWAVLNWNEPSIAFYKSLGARPMEDWSVFRLTGEKLDALAASA
                     "
     gene            complement(582..896)
                     /locus_tag="VNN25_05010"
     CDS             complement(582..896)
                     /locus_tag="VNN25_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HXH90918.1"
                     /translation="MSFAPIQKEVDDYISQFKEGYFPPLSMLARMTEELGELARALSH
                     QAGFKKPKSGEPAGEIADELCDLIFVAVCLANSLDIDLDNAFAAAMTKYRTRDADRWT
                     RK"
     gene            complement(913..1266)
                     /locus_tag="VNN25_05015"
     CDS             complement(913..1266)
                     /locus_tag="VNN25_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH90919.1"
                     /translation="MIGAFLFAVITSTAGPPGCPAGPIGRVGCCRDAPQLLTRVDEPY
                     RQVAERRHLHGIAIIELIIDTTGGVCAAHVLRGLDPEFDRAGLVAVRQWKFRPALNYS
                     GKPVTVAFNVTVKAK"
     gene            complement(1263..1739)
                     /locus_tag="VNN25_05020"
     CDS             complement(1263..1739)
                     /locus_tag="VNN25_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXH90920.1"
                     /translation="MLFLEFDDDELAAITRSMVMSRFAEGDAILEEGNANRALHVLRS
                     GRIRVSRRVGDSEVSLCDLVAGQTFGELSIIEDGVASATLRAVAETEVLSMPMHSLAQ
                     FLQEHPTAASKFWRAIAVELRRRLLQTNDVVSSYFEINRNLVENATFREAYAMCNR"
     gene            1890..2261
                     /locus_tag="VNN25_05025"
     CDS             1890..2261
                     /locus_tag="VNN25_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH90921.1"
                     /translation="MDGLLRNLIKTVRRRMKADEELTVEAVTKELGYTRQYVSGRFHR
                     TTGRLLSHFLKEKRLEKAARLLKGGNTRVSQIARRCGFDSENYFRQQFRERFGMSPRQ
                     FRANGTLRALSDRPLVQAQMA"
     gene            complement(2348..2938)
                     /locus_tag="VNN25_05030"
     CDS             complement(2348..2938)
                     /locus_tag="VNN25_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90922.1"
                     /translation="MRLGVITLGHVHEGELAALTPHLEHLEQLVLDVPFTDPLTKHRA
                     ELNRAVDAASDDWLLIVREREVVDEAIAGEIAAAMRAQTAWGFRIRSVPMYAGKPLRI
                     GMDEGELRLFHRRHLLRRGDLGVQGTVVRLNNVLRSITFESVDAHRDYLAKNGVPHSL
                     LRRLLLFVHHALVTRAFDRNTLQYLWIEAGYDHGAG"
     gene            complement(2941..3729)
                     /locus_tag="VNN25_05035"
     CDS             complement(2941..3729)
                     /locus_tag="VNN25_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXH90923.1"
                     /translation="MIRILYIGDVVGQPGRKALHQKLNHVVDREKIDFTIVNIENAAG
                     GFGVTEAIMEELSELPIQAYTSGNHIWDKKDFVENYDHYEHVIRPANYPQANPGKGRV
                     IRQTAAGVNVAVVQVMGNVFMPPCDNPFYVIDRELKEIGDAAKVIVVDIHCEATSEKQ
                     AMGRHLDGRASVVVGTHTHVPTADERILPMGTAFQTDVGMTGAYDSIIGFRPDEVLYR
                     FLMNTPKKLEVAKRDIRLTGAMIEVDETTGRASSIVRIQERVEE"
     gene            complement(3813..>4274)
                     /locus_tag="VNN25_05040"
     CDS             complement(3813..>4274)
                     /locus_tag="VNN25_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXH90924.1"
                     /translation="HDIGKSIDREMEGTHLELGRQVLEKYGEKREIIHAMECHHGDYD
                     PTTVEAVLVNAADALSAARPGARREILENYVHRLERLEGIANSMEGVSKSFAIQAGRE
                     LRIIVNAEKISDEQSIWLSKDVARKIEAELQYPGQIKVTVIREMRAVDYAK"
BASE COUNT          807 a         1356 c         1294 g          817 t
ORIGIN      
        1 aaccgacacg ccaacagcca ccacgcaaca cacaatcaac ctacgcatcc agatatcatc
       61 gcatgcacga tggcattcga aattcgagag gcaacggaat ccgatgtccc gctgatcctg
      121 cagttcattc gcgatctcgc cgaatacgaa cggcttgcgc acatggtcgt ggcgaccgag
      181 gaacaactgc gctccacgtt gttcggagct gtgcgcgtgg ccgaggtgtt gatcggctcc
      241 tcggacggcg tgcccgcagg cttcgcgatc ttcttccaca actactcgac gttcctcgcg
      301 cagccgggca tctatctcga ggatctattc gtcaagccgg agtttcgcgg ccgcggctac
      361 ggcaaagcgc tgctggttcg tctcgcgcaa atcgcccgcg accgcaactg cggccgcgtg
      421 gagtgggcgg tgctcaactg gaatgagccc tccatcgcgt tctacaagtc gctcggcgca
      481 cggccgatgg aggattggtc cgtgttccgg ctgacaggcg agaagctgga cgcactcgcg
      541 gcctcggcgt agcgcaaccg cgcaaccccg ctaccgcaga ctcacttcct cgtccaacga
      601 tccgcgtcgc gcgtccggta cttcgtcatc gccgccgcga aggcgttatc gagatcgatg
      661 tcgagagaat tcgcgaggca gacggcgacg aagatcagat cgcatagctc atcggctatc
      721 tcgcctgccg gctcgccgct cttcggcttc ttgaacccgg cctgatggct cagcgcgcgc
      781 gccagctcgc ccagctcctc ggtcatacgc gccagcatcg agagcggcgg gaagtagcct
      841 tccttgaatt gagagatgta gtcgtcgact tccttctgga ttggcgcgaa gctcatggcg
      901 gaagtgtatc ggtcacttcg ccttcacggt cacattgaat gcaacggtga ccggcttgcc
      961 ggagtagttc agggccgggc ggaacttcca ctgccgcact gcgacgaggc cggcgcggtc
     1021 gaactcaggg tcgagtccgc ggagaacgtg cgccgcgcaa acgccaccgg tcgtatcgat
     1081 gatcagctcg atgatggcga tgccgtgcag atgccggcgc tccgcaacct ggcggtaagg
     1141 ctcgtcgaca cgcgtaagaa gctggggcgc gtcgcggcaa catccgacgc ggccaatcgg
     1201 tccggccggg cagccgggag gaccagccgt cgatgtgatg acagcgaaga gaaacgcgcc
     1261 gatcatcggt tgcacatggc gtacgcctcg cggaacgtcg cgttctcgac gaggttgcgg
     1321 ttgatctcga agtagctgct gacgacgtcg ttggtctgca gcaggcgacg acggagttcg
     1381 acggcgatgg cgcgccagaa cttgctcgcg gcagtgggat gctcctgaag aaactgcgcc
     1441 agggagtgca tcggcatcga caggacctcg gtctcggcaa ccgcgcggag cgtggccgag
     1501 gcgacaccgt cctcgatgat cgaaagctcg ccaaacgtct gaccggcaac gagatcgcag
     1561 agcgagactt cgctgtcgcc gacgcggcgc gatacacgga tacggccgct gcgcaggaca
     1621 tggagggcgc gattagcgtt tccttcctcg aggattgcgt cgccctcggc gaatcggctc
     1681 atgaccatcg accgcgtgat tgcagccagc tcatcatcgt caaactcgag aaacaacatc
     1741 acgctgcgaa taaaagttga ctcttccacg agatctcccg ccgattctgg gtataatgtc
     1801 gccacactta attcgattaa tgccggatcg ggttgctgaa ccgcaagggt acagcggctc
     1861 gaccggcgca tcaacgaggg agggccgcga tggatggcct cttacgcaat ttgatcaaga
     1921 cggttcgccg ccgcatgaaa gcagatgaag agctcacggt cgaagcggtt accaaggagc
     1981 tcggctacac gaggcagtat gtctcaggcc gtttccaccg caccaccggc cgcctgctga
     2041 gccacttcct gaaggagaag cggctggaga aggcagcgcg cctgctgaag ggcgggaaca
     2101 cgcgcgtatc gcagatcgcc cggcgctgcg gtttcgactc ggagaactac ttccggcagc
     2161 agttccggga gcgcttcggc atgtcgccgc gccagttccg cgcaaacggc acgctgcggg
     2221 ctctgtcgga tcggccgctc gtgcaagcgc agatggcgta gtgcgttcga agcaatgcac
     2281 cacagagacg cagaggacac agagaaaacc tctgtgctct cagtgcctct gtggtgaaag
     2341 cttttaatca acccgcaccg tgatcgtacc ctgcctcgat ccagaggtat tgcagggtgt
     2401 tccgatcgaa cgcgcgcgtc accagagcat gatggacgaa gagaagtaac cggcggagca
     2461 gcgaatgcgg cacgccgttc ttcgccaggt agtcgcggtg cgcgtccacg gactcgaacg
     2521 tgattgatcg cagcacatta ttgagtcgca cgacggtgcc ttgaacgccg aggtcgccgc
     2581 ggcgcagaag gtggcgccga tgaaacaggc gcagctcacc ctcgtccata ccgatgcgca
     2641 atggtttgcc ggcgtacatc ggcacggaac ggatgcgaaa tccccacgcg gtttgcgcac
     2701 gcatcgccgc agcgatctcg ccggcgatcg cctcatcaac cacctcgcgc tcgcgcacga
     2761 tgagcagcca gtcgtccgat gccgcatcga cggcgcgatt caattccgcg cgatgcttcg
     2821 tcagcggatc ggtgaacggc acatcgagca cgagttgctc cagatgctcc agatgcggcg
     2881 tcagtgctgc gagctcgccc tcgtggacgt gcccgagcgt gatcacgccg aggcgcatgg
     2941 ctactcctcg actctctctt gaatccggac gatgctcgac gcgcggcccg tcgtctcgtc
     3001 gacttcgatc atcgcgcctg tcagccggat gtcgcgcttg gccacttcca gcttcttcgg
     3061 tgtgttcatc aggaagcgat acagcacctc atcgggacgg aagccgatga tcgagtcgta
     3121 cgcgcccgtc atgccgacat cggtctgaaa tgctgtaccc atcggcagta tccgctcgtc
     3181 cgcggtcggc acatgcgtgt gcgtcccgac gacgacggag gcgcggccgt cgagatgccg
     3241 ccccatcgcc tgcttctccg acgtggcttc gcagtggata tcgaccacga tgacctttgc
     3301 cgcgtcgccg atctctttca actcgcggtc gatgacatag aacgggttat cgcacggcgg
     3361 catgaacacg ttgcccatca cctggacgac cgcaacgttg acgcccgccg ccgtctgccg
     3421 gatcacgcgt cccttgcccg ggttggcctg cgggtagttc gccggccgga tcacgtgctc
     3481 gtagtgatcg tagttctcga cgaagtcctt cttgtcccag atgtgattgc cagaagtgta
     3541 agcctggatc ggaagctcgc tcagctcctc catgatcgcc tcggtcacac caaacccgcc
     3601 cgcggcgttc tcaatgttca cgatcgtgaa gtcgatcttc tcccgatcga ccacgtgatt
     3661 cagtttctgg tgcagtgcct tccggcccgg ctggccgacg acgtcgccga tgtagaggat
     3721 gcggatcatt ggttagtttc ggtcgctgac ttgctcagtt gctgggttgc tgcggtctcg
     3781 cacaacgacc gagcaaccca gaaccctggc cactacttgg cgtaatccac cgctctcatt
     3841 tcgcggatca cagtcacctt gatctgaccc ggatactgca actccgcttc gatcttgcgg
     3901 gcgacatctt tcgacagcca gatcgactgc tcgtcggaga tcttctccgc attgacgatg
     3961 atgcgaagct cgcgcccagc ctggatggcg aagctcttcg agacgccctc catcgagttg
     4021 gcgatccctt cgaggcgctc gaggcgatgc acgtagttct cgagaatctc acgacgtgcg
     4081 cccggacgtg ccgcggacaa cgcatcggcg gcgttgacca gcacggcctc gaccgtcgtc
     4141 ggatcgtaat cgccgtggtg gcattccatg gcgtggatga tttcgcgctt ctcgccatac
     4201 ttctcgagca cctggcgtcc gagctcgaga tgcgtccctt ccatctcgcg gtcgatcgac
     4261 ttgccgatgt cgtg
//
LOCUS       DATLVW010000155         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_377661, whole genome shotgun sequence.
ACCESSION   DATLVW010000155 DATLVW010000000
VERSION     DATLVW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377661"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..471)
                     /locus_tag="VNN25_05045"
     CDS             complement(127..471)
                     /locus_tag="VNN25_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90925.1"
                     /translation="MTHEAAVTISNRLFERNPAMWYRWLAFADSRGMTLLALIYVAVR
                     ELRDAAARAAFETGEDFSVITWKWREEIVGCKQCTQCTLPALATNSLRSTVSLVGGGQ
                     MANVESVKARGE"
     gene            complement(468..782)
                     /locus_tag="VNN25_05050"
     CDS             complement(468..782)
                     /locus_tag="VNN25_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90926.1"
                     /translation="MIREQQAGAFVAALEADDTDQILIVDDGEEPTATTPPALLLELT
                     EGTRGDPTPVPAELPVPLGREALIVIERFRFVLGYQCGWGAASIEEVIRNVRALMPRR
                     NR"
     gene            complement(779..1192)
                     /locus_tag="VNN25_05055"
     CDS             complement(779..1192)
                     /locus_tag="VNN25_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90927.1"
                     /translation="MGTAAQILTDSVNAAIAVGLTISLDSDMLGVVCTSSEAPLWEID
                     PMAKEISPLGAVLLATQPPLVDEDRALAHVFQSRPEFHEGIEDGACGRRNLARDDRLY
                     TEGHLLGVQVRELIQRRYGIPVERSSIEITEEIDA"
     gene            complement(1202..1882)
                     /locus_tag="VNN25_05060"
     CDS             complement(1202..1882)
                     /locus_tag="VNN25_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90928.1"
                     /translation="MKRKALFLITVLTATAACSQAPESIDDTSAPLFVDNHVTGGHGP
                     LSANGNQAAWGTVRLWSGSFGEDWLGSVDGNYIAHPTEYHTGDRPYVAMSPNFTFATA
                     YEFEAAPGVFYGRSSAITTSFVQFAYLPAAIVISYDDACPLVNATQWTTPHPCTGDWT
                     PFDGGGVPTFVIGNGIVKPGLPYGDYLKYTAPGGPGSWCTFNFSDLPNVAKPAGAPNH
                     AVAIDSCP"
     gene            complement(2030..2590)
                     /locus_tag="VNN25_05065"
     CDS             complement(2030..2590)
                     /locus_tag="VNN25_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXH90929.1"
                     /translation="MSVPIDQMTPDELRAEVIRLRSELTDREEIRGGKRSQSMVMLRR
                     LVEGAGLDRCLEWPGSRSKSGGYGRIYLRGTNYRAHRAAWELVNGPIPKGLLALHKCD
                     NPPCVNPHHLFLGTDADNMADAIAKGRRTIENGRRGFKLLPEQVVKIRKLRADGLSTY
                     ALGRQFGVTHNLVSEICNRKKWRSVK"
BASE COUNT          483 a          863 c          815 g          520 t
ORIGIN      
        1 tgcacttcac agcgccgcca gccgtcaaga tgagagtcgc aattgccaac tgaacgttgt
       61 gctcatcaag cggaccagcc gccatcgccc tgccgcacgt gtcgcaaaca tcgaccaagt
      121 aagtgctcat tcgcccctcg ctttcacgct ctccacattc gccatttgcc cgccgcctac
      181 cagcgaaacc gtcgaacgca gcgaattcgt tgccaaagcc ggcagcgtgc attgcgtgca
      241 ttgtttgcaa ccgacgattt cctcgcgcca cttccaggtg atcacgctga agtcctcgcc
      301 cgtctcgaac gcggcccgcg cagcggcgtc gcgcagttcg cggaccgcca cgtagatcag
      361 tgccagcaac gtcatcccgc gagagtcggc gaacgcgagc cagcgatacc acatggccgg
      421 attgcgctcg aacagtcgat tcgagatggt aaccgccgcc tcgtgtgtca tcggtttctc
      481 cgcggcatca gagccctcac gttgcgaatt acctcctcga tcgatgccgc gccccacccg
      541 cactggtagc cgagcacgaa gcggaagcgc tcgatgacga tcagcgcctc gcggccgaga
      601 gggacgggca gttccgcggg cacgggagtc ggatcgccgc gcgttccctc ggtaagctcg
      661 agcagcaagg ccggtggcgt cgtcgcggtc ggctcctccc cgtcgtcaac gatcagtatc
      721 tgatcggtgt catccgcctc cagcgccgcc acaaacgccc cggcctgctg ctcccgaatc
      781 atgcgtcgat ctcctcggtg atttcgatgc tcgatcgctc gacggggatg ccgtaccgcc
      841 gctgaatcag ctcgcgaacc tgcacgccga ggagatgtcc ctcggtgtag agccgatcat
      901 cgcgtgcgag gttgcgccgc ccgcacgcac catcctcgat cccctcatgg aactctggcc
      961 gcgactggaa cacgtgcgcg agcgcgcggt cctcgtcgac gagcggtggc tgagtggcga
     1021 ggagaaccgc cccgagcggc gagatctcct tcgccatcgg gtcgatctcc cagagcggcg
     1081 cttcgctcga cgtgcagacc acgccgagca tgtcggagtc tagagagatc gtgagaccga
     1141 cggcgatagc cgcgttcacg ctatcggtga ggatctgtgc tgcggtgccc atggtgcagc
     1201 cttacgggca gctgtcgatc gcgaccgcgt gattcggagc acccgcaggc ttcgccacgt
     1261 tgggcagatc ggagaaattg aacgtgcacc acgagcccgg gccgccgggt gccgtgtact
     1321 tgaggtaatc gccgtagggc aacccaggct tgacgatgcc gttgccgatc acgaacgtgg
     1381 gcacgccgcc gccgtcgaat ggggtccagt cgcccgtgca gggatgcggc gtggtccact
     1441 gcgtcgcatt caccagcgga caggcgtcat cgtagctaat gacgatcgcg gcagggagat
     1501 atgcgaactg aacgaatgag gtggtgatcg cgctggaacg tccgtagaac acgccaggcg
     1561 ctgcctcgaa ctcataggcc gtcgcaaacg tgaagttggg cgacatcgcg acgtagggcc
     1621 tatctcccgt gtggtattcc gtcggatgcg cgatgtagtt accgtctacg ctcccaagcc
     1681 agtcctcgcc gaagctaccc gaccagagtc ggacggttcc ccatgccgcc tggttgccgt
     1741 tggcggacag tggaccatgc ccgccggtta catggttgtc gacgaacagt ggggcagacg
     1801 tgtcgtcgat cgattctggc gcctgagaac aggctgcggt cgcggtcaga acggtgatca
     1861 ggaacagtgc ttttcgtttc attgggtctc ctcttgacag ggagacctgc gcgcacgtat
     1921 gaccgccggt tgatggggcc gggctgtcct gcgcgagcag gtcggttgcg gttctcggcc
     1981 ccgtccctcg ctccaacggg ggacggggca tttactaaaa acgccggtcc tacttgactg
     2041 acctccattt ctttcggttg cagatttcgc tgactaggtt gtgcgtgact ccgaattgac
     2101 ggccaagcgc ataagtgctg agtccgtcag cacgaagctt cctgattttg acgacttgct
     2161 caggtagcaa cttgaatccg cggcgtccgt tctcgatcgt tcgtcttcct ttggctatcg
     2221 catcggccat gttgtcggcg tcggtaccaa ggaataggtg atgcgggttc acgcagggcg
     2281 ggttgtcgca tttgtgaagc gcgagcagac ccttcggaat cgggccattg accaattccc
     2341 atgcagctcg atgcgcgcgg tagttggttc ctcgcagata gatccgtcca tacccgccag
     2401 actttgaccg cgaaccgggc cattccaggc atctatctag accggcacct tcgacaaggc
     2461 ggcgcaacat aaccatcgac tgcgatcgtt tgccgccacg gatttcttca cggtcggtca
     2521 gttcagatcg aaggcgtatg acttcggcgc gtaactcatc gggcgtcatc tgatcgatcg
     2581 gaacagacat cacgccgtct cccggaccgc gtcgcacagc agcgaccacg tgaggttgct
     2641 cggcgccttg acgcccgccg cgcgcgccca ccgctcgcgc t
//
LOCUS       DATLVW010000156         4498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_584362, whole genome shotgun sequence.
ACCESSION   DATLVW010000156 DATLVW010000000
VERSION     DATLVW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584362"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VNN25_05070"
     CDS             complement(<1..227)
                     /locus_tag="VNN25_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90930.1"
                     /translation="MAAKNRPYEQFGTFILFKKLDTDSLGELWRAARLDGSTLSPILA
                     LRRMSGGNRTAMTQSASEASQLVPLLSGTTFV"
     gene            complement(293..1327)
                     /gene="mltG"
                     /locus_tag="VNN25_05075"
     CDS             complement(293..1327)
                     /gene="mltG"
                     /locus_tag="VNN25_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXH90931.1"
                     /translation="MTIGLFAAVLAVAGGWFFIRAMRSPFRGYTEPYKDVEVRHGLRT
                     ATILKHLETEGIVRDEYLPLVYMKIVRHNDKIKAGIYEFSKPMTAEEVLDKLVRGDVV
                     AKSVTVREGLDRFAVAHIFVQEGFGRDAEWDKLTADPELIRDIAPDAKTLEGYLFPDT
                     YKFNPGTPPHEIIAAMVNNFRKHFGAEMNYITTGLDLHKTVTLASIVETEARMPQERP
                     LVASVYLNRINRHMLLGADPTVIYALKLAGHWDGNIRKGDLQIDSPFNTYRFHGLPPG
                     PIANPGLASLQAAAAPAKSDFLYFVAKHDGTHAFATNLADHDRNVQIYQIQWYRDQRA
                     AQQAQSQTTK"
     gene            complement(1378..1785)
                     /gene="ruvX"
                     /locus_tag="VNN25_05080"
     CDS             complement(1378..1785)
                     /gene="ruvX"
                     /locus_tag="VNN25_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXH90932.1"
                     /translation="MSIIAIDYGGRRIGVAVSDSGAIARPHSVVKNEGDVIEKLASLA
                     KDLEAETIVLGVPRRPNSSGGEQKFRDFAERLRQKTCKVVVLWDEALTTSEASRQLRD
                     HGMKRREAQREIDMHAAAVILQSWLDDAARRTS"
     gene            1840..2988
                     /locus_tag="VNN25_05085"
     CDS             1840..2988
                     /locus_tag="VNN25_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXH90933.1"
                     /translation="MKNFNLDAILEAMLKMSDRVSDLNFSVGRPPQVEIDGQLAPVNF
                     PGLPRLTPYQTEMIAMHMLRGDRELTRTLLTNGSVDLSYSIPQKTRFRVNIFAQRGTY
                     AIVLRVIPEGIPSLESLKLPAELGNICKLKNGIVLVTGPTGSGKSTTLAALINKINNE
                     YAYHIITIEDPVEYMHKHIKSTVNQRELGTDTKSFALALRAALRQAPKVILIGEMRDV
                     ETIEIAMEAAETGHLVLSTLHTIDAARTIDRIVGVFPKDQEAQVRTRFAQSFRYVVSQ
                     RLLPKRGGGRLAVMEILKSTMRTRDYVLRGETDGRSITDAMHDGSVDGMQTFDDELEK
                     LWNERVITREIALAYASNPTNLALRLTDEPEAAPPAGSDGDSMLSMLE"
     gene            2992..3621
                     /locus_tag="VNN25_05090"
     CDS             2992..3621
                     /locus_tag="VNN25_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90934.1"
                     /translation="MKIVCTSCQKPLTLDETRLPMKPVLIPCPVCQTKLTVDRRNLEM
                     AGAVASGQPAAATQTAEPLTQQHEEDDGFGAKALIVGLDHPALRQAAKMIGFMPVFFA
                     SPQQARDYFMQEYPPLVMLHPAQITPPPLVDFGPIVSLAPTDRRKAFFILVSDNLRTL
                     DGNAAFLYGINLVIAAKDLGAFQQIYREAHGYHERLYAPLAAALKAMES"
     gene            complement(3668..4147)
                     /locus_tag="VNN25_05095"
     CDS             complement(3668..4147)
                     /locus_tag="VNN25_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH90935.1"
                     /translation="MIRKNQKGFTLIELLIVVAIIGILAAIAIPNLLTAMARSKQKRT
                     MADMRTIATAWEARATDVNKYSAAGACSIPTTSSSPTDMGGILSPTYIKVLPPRDGWN
                     TGWEYRVGTQTYAIISYGKDQTAAASYATCGPTTNFDCDIVYAEGTFLIYPDGVQTQ"
BASE COUNT          835 a         1323 c         1413 g          927 t
ORIGIN      
        1 acgaatgtcg ttccgctgag gagcggcacc aactggctcg cctcggacgc gctttgcgtc
       61 atcgcggtac ggttgccgcc gctcatccgc cgcagagcga gaatcggact gagagttgat
      121 ccatcgaggc gggcagcgcg ccagagctca ccgagcgaat cggtgtccag tttcttgaat
      181 aggatgaacg ttccgaactg ctcgtacgga cggtttttgg cagccatcta tcgggaccct
      241 ctcggcttgt tggcggtccg cggaggatag cacaaaggtt attggcagtg agttatttgg
      301 tagtttgcga ctgcgcttgc tgcgccgcac gctgatcgcg ataccactgg atctggtaga
      361 tctgcacgtt gcgatcgtgg tccgccaggt tcgtggcgaa tgcatgtgta ccgtcatgtt
      421 tggcgacgaa gtagagaaag tcgctcttag ccggcgcggc ggcggcctgg agcgacgcca
      481 gccccggatt ggcgatcgga ccaggcggca agccgtggaa acgataggtg ttgaacggcg
      541 aatcgatctg cagatcgccc ttgcggatgt ttccgtccca gtggccggcg agcttgagcg
      601 cgtaaataac ggtgggatcg gcgccgagga gcatgtggcg attgattcgg ttgaggtaga
      661 ccgatgccac gagggggcgc tcctgcggca tgcgggcctc ggtctcgaca atggaagcca
      721 gcgtgaccgt tttgtgcagg tcgagaccgg tggtgatgta gttcatctcg gcgccgaagt
      781 gcttgcggaa gttgttgacc attgccgcga tgatttcgtg gggcggtgtg ccgggattga
      841 acttgtaggt gtcggggaag aggtagccct cgagggtttt cgcatcgggg gcgatatcgc
      901 ggatcaattc gggatctgcg gtcagcttgt cccactcggc atcgcgtcca aacccttcct
      961 gtacgaagat gtgtgcaacc gcaaagcggt ccagtccttc gcggactgtc acgctcttgg
     1021 cgacgacatc gccgcgaacc agtttgtcca atacttcttc ggccgtcatc ggcttcgaga
     1081 actcgtagat ccccgctttg atcttgtcgt tgtgccggac gatcttcatg tagacgagag
     1141 gaaggtactc gtcgcgaacg attccctcgg tttccaggtg cttgaggatc gttgcggtac
     1201 ggagtccatg gcgtacttcg acgtctttgt atggctccgt atagcctcgg aacggcgagc
     1261 gcatcgcccg gatgaagaac cagccgccgg caacggccag gaccgcggcg aagaggccga
     1321 tggtcatcaa cgactgcagg atcttccggc agccgccttg cctgcgtgcg cggcgagcta
     1381 tgacgtcctc cgggctgcgt cgtcgagcca ggactggaga atcaccgccg cggcgtgcat
     1441 gtcgatttct cgctgagcct cgcgccgttt catcccatgg tcgcgcaatt gccgtgaggc
     1501 ctcggacgtc gtgagcgctt catcccagag cacgaccact ttgcacgttt tctgccgcag
     1561 ccgttcggcg aagtcgcgga acttctgttc gccgccgctg ctgttgggcc gccgaggtac
     1621 gccgagcacg atggtctcgg cctccagatc cttcgccaga ctggccagct tttcgatgac
     1681 gtctccttcg ttcttcacga cagaatgggg tcgggcaatc gcgccggagt cgctgaccgc
     1741 cacaccgatg cggcggcctc cgtagtcgat ggcgatgatg gacatgttcg cgattgtact
     1801 tggccgtaca acgtacaatg gcagaacccg aggacgcaga tgaagaactt caacctggac
     1861 gccattctcg aggcgatgct gaagatgtca gatcgggtgt ccgacctcaa cttttcggtc
     1921 gggcgtccgc cgcaggtcga gatcgacggg cagctcgcgc cggtcaactt ccccggactc
     1981 cctcggctga cgccatacca gacggagatg atcgccatgc acatgctgcg cggcgaccgc
     2041 gagctcacgc gtacgctgct gacgaacgga tcggtcgatc tctcgtactc gattccgcag
     2101 aagacccggt tccgcgtcaa catctttgcc cagcgtggca cctacgcgat cgttcttcgc
     2161 gtcattccgg aagggattcc gtcgctcgag tcgctgaagc tgcccgcgga gctcgggaac
     2221 atctgcaagc tgaagaatgg catcgtcctg gtgacgggac caaccggctc gggcaaatcg
     2281 acgacgctgg ccgccctgat caacaagatc aacaacgagt atgcgtatca catcatcacg
     2341 atcgaagatc cggtcgagta catgcacaag cacatcaagt cgaccgtgaa tcagcgggag
     2401 ttaggtaccg atacgaagtc gttcgcgctt gctctgcgcg ccgcgctgcg gcaggcgccg
     2461 aaagtgatcc tcatcggcga gatgcgcgac gtcgaaacga tcgagatcgc gatggaagcc
     2521 gccgagaccg gacatctcgt tttgtcgacg ctgcacacga tcgatgcggc cagaacgatc
     2581 gaccggatcg tgggcgtctt tccgaaggat caggaggcgc aggtgcgcac gcggttcgcg
     2641 cagtcgttcc ggtatgtcgt ctcgcagcgt cttctgccga agaggggtgg aggccggctg
     2701 gcagtgatgg agatcctcaa atcgacgatg cggactcggg attacgtgct ccgaggggag
     2761 acggatggac gctcgatcac cgacgccatg cacgatggtt cggtggacgg gatgcagacc
     2821 ttcgatgacg agctcgagaa gctgtggaac gagcgcgtga tcacgagaga gatcgcgctc
     2881 gcctatgcgt cgaacccgac caacctcgcg ctgcgcctca cggacgagcc ggaagccgcg
     2941 ccgccggccg gaagcgatgg cgattcgatg ctgtcgatgc tggagtaagc gatgaagatc
     3001 gtctgcacgt cctgccagaa gccgctcacc ctcgacgaga cccggttgcc gatgaagccg
     3061 gtcctcattc cgtgtcccgt ctgtcaaacc aagctgacgg tcgaccgccg caacctggag
     3121 atggccggcg cggtggcatc gggacagcca gctgcagcaa cgcagacggc ggaaccgctc
     3181 actcagcaac acgaggaaga cgacggattc ggcgcgaagg cgctgatcgt cggcctcgat
     3241 catcctgctc tgcgtcaggc cgcgaagatg atcgggttca tgcctgtctt cttcgcctcg
     3301 ccgcagcagg cacgcgacta cttcatgcag gagtatccgc cgctggtgat gctccatccc
     3361 gcgcagatca cgccgccgcc gctcgtcgat ttcgggccga tcgtctcgct cgcgccaacg
     3421 gatcggcgga aggcgttctt catcctcgtt tccgacaacc tgcgcacgct cgacggcaac
     3481 gccgcgtttc tgtacggcat aaacctcgtg atcgctgcga aagacctcgg tgcctttcag
     3541 cagatctacc gcgaggcgca cggctaccac gaacggcttt atgcgccgct cgctgcggcg
     3601 ctcaaagcga tggagtcata aaaaagggcg ccttgcggcg ccctccgttc cttcactctg
     3661 tcggatgtta ctgggtctgc actccgtccg gatagatgag gaaggtaccc tcggcgtaga
     3721 cgatgtcgca gtcgaagttc gtcgtcggtc cgcatgtcgc gtaggaggcg gcggccgtct
     3781 ggtccttccc gtacgagatg atcgcgtacg tctgggtgcc gaccctgtac tcccaacccg
     3841 tgttccagcc gtctctcggc ggtagaacct tgatgtaggt cggtgacagg attccgccca
     3901 tgtccgtggg cgaggacgat gtcgttggga tgctgcatgc acctgctgcg ctgtacttgt
     3961 tcacgtcggt agcgcgggct tcccaagcgg tggcgatcgt acgcatgtcg gccatcgttc
     4021 gtttctgctt cgaccgtgcc attgccgtga gcagatttgg aatcgcgatg gccgcgagaa
     4081 tgccgatgat cgccacgacg atcagcagct cgatgagagt gaaacccttc tgattctttc
     4141 taatcatgaa tgaacctcct aatgtgcatc gaccatcgca atcgggatgc cgatcggctt
     4201 gcttgaacaa cccgcttgaa atcagatagt tacacgccgt gttcacgacc gcttggcgct
     4261 ttcttgacgt ttttcgttag tgggcatgga caaattccgt cagtgtcgga tatttggacg
     4321 ccaattgccg ctttgggccg gtgagcaggc tggggatgcc cggcgttgga gcactgaacc
     4381 gtcaaaacag aaagggcgcc cgagggcgcc ctttcgtttt gttgcttgaa ccgcgcctgg
     4441 attactgggt ctgcacgccg tccgggtaga tcaggaacgt accctcggca tagacgat
//
LOCUS       DATLVW010000157         7712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_543002, whole genome shotgun sequence.
ACCESSION   DATLVW010000157 DATLVW010000000
VERSION     DATLVW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543002"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VNN25_05100"
     CDS             <1..512
                     /locus_tag="VNN25_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HXH90936.1"
                     /translation="GLEASPLRRYDASTMTKAAACPISYETVDHNASRVTCILTIVAL
                     AAISSMAVLWPGLGTIALILAAALTLDYAIRAWTRYPSPMQSLGRGFARAFGIPEKRS
                     DAAPKRFACRIGFFFALGVVAFLPVAPRMAVMVAGALLFFNVLDGVFDFCVGCWMYTM
                     FLLPRGTDS"
     gene            complement(604..873)
                     /locus_tag="VNN25_05105"
     CDS             complement(604..873)
                     /locus_tag="VNN25_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90937.1"
                     /translation="MTLDEALADAIRRDHASAPVSEQDHVMLAYVSQLTRDATAIQRE
                     DHDRLRAVGFDDVAILQITLIASWFNYINRVADALGVGRDSGGGA"
     gene            complement(904..1185)
                     /locus_tag="VNN25_05110"
     CDS             complement(904..1185)
                     /locus_tag="VNN25_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90938.1"
                     /translation="MTWIRTISFPEADDTLRKAIEGQRALYPAEYATPGFPGQLPGQG
                     IVDAHSLLPDALFQAFATFGALMAPNLPLERRQHEMIATVVSATNRCHY"
     gene            complement(1154..1498)
                     /locus_tag="VNN25_05115"
     CDS             complement(1154..1498)
                     /locus_tag="VNN25_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH90939.1"
                     /translation="MTRSRQTDPDALVFGAILRRERLARGWTIVKLARRAGMNSQYLG
                     VIEFGRNSPTLATILELAEVLGADAGELVREVAAARKKPKVPQTQAGARRSVSTSTGG
                     ERHDMDPDDQLP"
     gene            2224..3021
                     /locus_tag="VNN25_05120"
     CDS             2224..3021
                     /locus_tag="VNN25_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH90940.1"
                     /translation="MVRSAITSLVCLAALMSGCAATARQSSPTEGTSPAAHVQLLTIE
                     LRDHPDPHTGALRVIDGARPWPLELGALLDAVSHDWAPTSVYDVRRARLAAGVIEQTE
                     ETTVRVLESNRNSARLSIKLDAAAQPIEVTVPINSTVVVGSEAGGQRHAFVALSLLDR
                     TTRMRIPEVFSTREPGIVPPLQLDHSPLAAPESARANGQKGVLLDVEIDERGNVIRAH
                     VLGHRFLSDTEIAAIEKTVSGWKFKPGTANGKAVRVVTTITSVFREP"
     gene            3851..4417
                     /locus_tag="VNN25_05125"
     CDS             3851..4417
                     /locus_tag="VNN25_05125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH90941.1"
                     /translation="MIAQPLLHECSDPLRSTFAAAHDRIRRGATLLLLLALTIAPCAF
                     AKSQERLTQAPDFSLPGASGTVSLHDFRGKVVLIDFWASWCVPCRQSFPWMTSMMNRY
                     GAQGLEIIAVNLDKKRDAADRFIEAFPATFPVVFDPSGKTAEGFRVEAMPSSFLVSRT
                     GEIIHIYAGFDPGHVQTIETQIQEALSK"
     gene            4423..4641
                     /locus_tag="VNN25_05130"
     CDS             4423..4641
                     /locus_tag="VNN25_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011043595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HXH90942.1"
                     /translation="MTRYLWRFIATAALLLVAGCATVQPWDRDLLAEKKMSLNPSPML
                     QAVDEHIYFSKEASMGGMDAGGGGCGCN"
     gene            4626..5891
                     /locus_tag="VNN25_05135"
     CDS             4626..5891
                     /locus_tag="VNN25_05135"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HXH90943.1"
                     /translation="MRLQLELGKAPKVSLRDALHGAALALLIIPATAQAQSTPSDRID
                     VTSLLYGEQGRTEVVEPVVRFTHLLPDGQSVSAQLAFDVITGASPSGALPSGTVQTRT
                     SASGHVITSKAGQIPVAPFHDERVELEGTWHKPLNRLISSDLGFHVSREKDYQSLGVN
                     AKVSTELMRRLLTVTVGGGVSHDTVFPTGGTPAGLSDGTIVKLGSNAKNVTNVLLGAS
                     RIVSRQWLVAVNGTETLERGYLTEPYKVISIMSHVNGTPIGELTDKRPSTRNRSAVLL
                     SSVYHLTDDVLYTSYRYYWDTWSIRSHTIDLKYRHDLGDDSYIEPLLRYYQQSAASFY
                     TVGFVEGATLPDFATSDYRLGRLRTITAGATYSFRPDFATGEFTVRGQYMRQFGNGSP
                     SNAIGIQRNFDLSPPVNIVAVVLGYSFDY"
     gene            5899..6825
                     /locus_tag="VNN25_05140"
     CDS             5899..6825
                     /locus_tag="VNN25_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXH90944.1"
                     /translation="MQAAARSAEQEATDDAPDLRRVDDYFIGGFHAMASPCTLLIDTD
                     DRTEAGRCLAIASAEAIRIERKFSRYRRDNIVYDINHAEGRPVVVDHETALLLNYAAT
                     CYVISDGMFDITSGVLRRVWTFDGGCKVPSRQSVEACLRHLGWQRVAWKNPVLTIPVG
                     MEIDFGGIGKEYAVDRAAALIASRTKSSFVVNFGGDLYTAGLRRDGRTWAIGIDDPGH
                     TGKSALYRLDVPKGGIATSGDARRFVQWRGKRLGHILNPKTGWPVEGAPRSITVFGRT
                     CMEAGTLSTLAYLQGRNAKQFLEQQGVQFWIV"
     gene            complement(7404..>7712)
                     /locus_tag="VNN25_05145"
     CDS             complement(7404..>7712)
                     /locus_tag="VNN25_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90945.1"
                     /translation="DEVIADALLAFLNSSFVGWYLDQRTPAFRGGYKKFEVQHLTDIP
                     IPRAFFDIEIQDCLTRLARDASFFHIANPPDTRSDAEAKIDEIVNSLLPETERANLRS
                     "
BASE COUNT         1612 a         2379 c         2205 g         1516 t
ORIGIN      
        1 agggtttgga agcgagccca ttgcggcgtt acgatgccag cacgatgacc aaagccgctg
       61 cctgcccgat ctcgtacgag accgtcgatc acaacgccag ccgcgtgacg tgcatcctga
      121 cgatcgttgc tctggctgcg atctcttcga tggcggtgct gtggcccggt ctcggaacga
      181 ttgcgttgat cctcgccgcc gcgctgacgc tggactacgc gatccgtgcc tggaccagat
      241 acccgagccc gatgcagagc ctcgggcgcg gtttcgcgcg cgcgttcggc atccctgaga
      301 agcgaagcga cgccgcaccg aagcgcttcg cctgccgcat cggcttcttc ttcgccctcg
      361 gcgtcgtggc gttcttgccc gtggctccca ggatggcggt catggtggcg ggagctctgc
      421 tgtttttcaa cgtgctcgac ggcgtgttcg acttctgcgt cggctgctgg atgtacacga
      481 tgttcctgtt gccgaggggt acggactcct gagccggcgt ttctctgcat tctccgcggc
      541 tccgcgtgaa actcaaaacc cttttttcac gaggaggcgc ggagaacgcg gagaaaaaga
      601 agctcaggcg ccgccacctg aatcgcggcc gacaccaagc gcatccgcga cgcgattgat
      661 gtagttgaac cacgatgcga tcagcgtgat ctgcaggatt gcgacgtcgt cgaaccccac
      721 cgcacgcagc cgatcgtggt cttcgcgctg tatggctgtg gcatctcgcg tgagctgcga
      781 gacgtacgcc agcattacgt gatcctgctc cgagacagga gcgctggcgt gatcgcggcg
      841 gatcgcgtcc gccagcgcct cgtccagggt cacccgacgc agaaactctg cgtgcgagac
      901 gattcaatag tggcagcgat tggttgccga tacgacggtg gcaatcatct cgtgctgccg
      961 gcgctccagc ggcaggttcg gcgccattag tgcaccaaac gtcgcgaacg cttggaacaa
     1021 cgcatcgggc aggaggctgt gcgcgtccac gatgccctgg cccggcaact gcccgggaaa
     1081 tcccggcgtg gcgtactcgg cgggatagag agctcgctgc ccctcgatcg ccttgcgcaa
     1141 cgtgtcatcc gcctcaggga agctgatcgt ccggatccat gtcatggcgt tcgcccccag
     1201 tactcgtcga tacgctgcgt cgcgcgcccg cctgcgtctg cgggaccttc ggcttcttcc
     1261 gcgccgctgc cacttcgcgg accaactccc ccgcatccgc tccgagcacc tcggccaact
     1321 ccaggatggt cgcgagggtg gggctgttcc taccgaactc gatcacgccg aggtattggg
     1381 aattcatccc ggcgcgccgg gcaagcttga cgatggtcca cccgcgagcc aggcgctcgc
     1441 gccggaggat cgctccgaag acaagggcat caggatcagt ctgtcgagat cgcgtcatga
     1501 ctcggataat atatccatgg gcattccaat tacacagatt tgcctatcaa agatgtccat
     1561 agacatcgaa catcagcaga ttgactgcct gaatttgtcc atggccatat ttcgccagtc
     1621 aaaacgacta ctcaaatttg ccatggcatg atttaaaaat tgccatagca atattaaacg
     1681 aaggcaactt cgtcaaaaag atgtccatgg atataaatcg gacatcatca agcttgtcga
     1741 aagttgccat ggccatattt cgtcagtcaa aactacaacc gaaatttgcc atagcatgat
     1801 taagcagtgc cgaatcatgg ttaaacggac gacacttcgt ccaagaatat gtccatgggt
     1861 ataaatcaga catcatcaag cctactgaaa attgccatgg ccatatttcg acagcctacg
     1921 cgacctaccg aataagtcca tagcatgaat tcgccaggca ggatgactac cgaaagatct
     1981 ttgatgatct actcatcaac gattttcgcg tccgctcgag acgcagcgat ggttcacgag
     2041 ccgctctgcc tcgctttcac ccaacgaacc ggcgcgccgc tgcgtttctc aagcgaaagg
     2101 tggctgcccg cgcgtctcaa tgtgcgcgaa agatgcatcg cggtctccgg ttccgaggcc
     2161 cggcgtggac tcggattggg gggcgggcat tcgtcgcgcc tgatgatcag ggtggaggga
     2221 ctcatggttc gctcagcgat cacttctctc gtatgtttgg cggccttgat gagcggatgc
     2281 gcggcaacgg ccaggcaaag ttccccaacg gagggaacga gcccggcggc gcacgttcag
     2341 ttgctcacga tcgagctccg ggatcatccc gatccgcaca ccggcgcgct tcgcgtcatc
     2401 gacggcgcgc gcccgtggcc tctggaactg ggagccctcc tggatgccgt gtcgcacgat
     2461 tgggcgccaa catcggtcta cgacgttcgg cgcgccaggt tagcagccgg cgtcatcgag
     2521 caaaccgagg agacgacggt ccgcgttctc gagtcgaatc gcaattccgc gcgcctatcc
     2581 atcaagctcg acgcggcagc gcagccgatc gaggtcaccg tcccgatcaa cagcaccgtc
     2641 gtcgtcggca gtgaagccgg cggccagcga cacgcattcg tcgccctctc cctcctcgac
     2701 cgcactacgc gaatgcgcat ccccgaagtc ttcagcacac gcgagccagg aatcgtcccg
     2761 ccgctgcaac tggatcactc accgctcgca gctccagagt cagcccgcgc gaacgggcaa
     2821 aagggcgtgt tactcgacgt cgagatcgac gaacgtggca atgtcatcag agcccacgtc
     2881 ctcggccacc ggttcctctc tgacacggaa atcgctgcga tcgagaagac ggtgtctggg
     2941 tggaagttca agcctggtac cgcaaacgga aaagctgtgc gggtcgtgac gacgattacg
     3001 tctgtctttc gcgagccttg acgactgcaa cctttctcac acgcttatcg caactctttc
     3061 caaccgagcg ccttctcccc cacgcttttc agtgggggag aaggtgccga aggcggatga
     3121 gggggcgttt caacaagcag acatccgtgc atgtgccacg ttttcaggca gttcgtacga
     3181 ggcgtggttg gtcgccctgc gcatctacgc ccccctcatc cggcttcgcc accttctccc
     3241 cccgcaaaaa gcgcgggggg agaaggcgct cgattgggaa agcactccaa gaaaggaaga
     3301 ggtgtagcag ggcgcgcagc aaggacgtgc aaccgtggga gaaatgcggg ttaacccggg
     3361 ttacgccttc gctcccactc cccacaccct actccccacc cccgaaccct tacggcgagt
     3421 gagcctgatc ccgcgaaccg cacaaatcaa tcgtgcgata aacgatcttt cgcgcgcgtg
     3481 gatcgtaacg ttcttcccgt aaggccacga gccgcgagtc gtgaaaggtc aggtcgacca
     3541 agctgaggtc tttcctgttc cccgacgggc tcgccgccga gcgcgcgagc caggaagggt
     3601 ctgatccttt gtagcctttc tcgcgcgcca gttgcgtgac ctgttcgcgg gtcatgccgc
     3661 atcgaagcga atcccggaat gattgcgcag catcgggccg ctgacacaca gccgagctga
     3721 gcagaatgac ggcgatcaat agcactcgtg tcgctcgtct ctgcatcgat gtgaacaaac
     3781 taacacagcg tacccactac gaaacgcggt taatgccgcg tttatgagcg tgcgcggatg
     3841 atcgccagcg atgatcgccc aaccactcct gcacgaatgt tccgacccgc tccgttcgac
     3901 cttcgcagcc gctcatgacc gcatccggcg cggcgcaacg cttctgcttc tgctcgctct
     3961 gacaatcgca ccatgcgcct tcgcgaaaag ccaggaacgc ctgacacagg cgcccgactt
     4021 ttcgctgcct ggagccagcg gaacggtctc gttgcacgat tttcgcggca aagtcgttct
     4081 tatcgacttc tgggcgtcgt ggtgtgttcc ctgccgccag tcctttccct ggatgactag
     4141 catgatgaat cgttacggcg cgcaaggcct cgagatcatc gcagtgaacc tcgacaagaa
     4201 gcgagacgcc gcggacaggt ttatcgaagc ctttccagcg acttttcctg tcgtatttga
     4261 tccctccgga aaaaccgccg agggatttcg cgtagaggct atgccatcga gcttcctcgt
     4321 gagccgcacg ggcgagatca tccacatata cgcaggattc gatcccgggc acgtgcaaac
     4381 catcgaaacg cagatccagg aggcattatc aaaatgaaaa gaatgaccag gtatctctgg
     4441 cgattcattg caaccgcggc gctcctgctc gtcgctggat gcgcgaccgt tcaaccgtgg
     4501 gatcgcgatc ttctcgccga aaagaaaatg agtctgaatc cctcccccat gctgcaggcg
     4561 gtggacgagc acatctactt cagcaaagag gcttccatgg gcggaatgga tgctggaggc
     4621 ggcggatgcg gttgcaatta gagctgggca aggctcccaa agtctccttg cgcgatgcgt
     4681 tacacggcgc tgcattagcg ctgctcataa ttccggctac agcacaagct cagtccaccc
     4741 cttcggaccg catcgatgtc acgtcactgc tgtacggaga acagggccgc accgaggtgg
     4801 tcgaaccggt cgttcgcttc acacatctgc taccggacgg acagtcggtc tccgcgcaac
     4861 tggcattcga cgtcatcacc ggagcctcac ccagcggagc gctgccttcc ggaacggttc
     4921 aaacccgcac cagcgcgtca ggccatgtca ttacgtcaaa ggccgggcag attccggtgg
     4981 cccctttcca cgatgagcgc gtggagctgg agggcacatg gcacaagccc ttaaaccgcc
     5041 tcatcagctc cgacctcggc ttccacgtct cccgggagaa ggactaccaa tcgcttggcg
     5101 tcaatgcaaa agtgtcgaca gagttgatgc gccggcttct cactgtcact gtcggcggcg
     5161 gcgtcagtca cgacaccgta tttccaaccg ggggcacgcc agcgggatta tccgacggga
     5221 ctatcgtcaa gctcggaagc aatgccaaga acgtcaccaa tgtattgctc ggcgcctccc
     5281 gcatcgtgag ccgtcaatgg ctcgtggccg tcaacggcac cgagacgctc gagcgcggat
     5341 atctgacgga gccttacaaa gtcatcagca tcatgagtca cgtgaatggc acgcccatcg
     5401 gcgagctgac cgataagcgg ccctccacgc gcaaccggtc agccgtgctg ctgagctccg
     5461 tctatcacct gaccgacgat gtgctctata cgtcgtatcg ctattactgg gatacctgga
     5521 gcatccgttc ccacaccatc gatctgaaat accgtcacga cctgggcgac gattcctaca
     5581 ttgagccgct cctccgctat taccagcaga gcgcggccag cttctacacg gtcggattcg
     5641 tagaaggcgc gacgttgccg gactttgcga cgagtgatta caggctgggc agattgcgaa
     5701 cgatcacagc aggtgccacg tatagcttcc ggcccgactt cgcgactggc gaattcaccg
     5761 tgcgcggcca gtacatgcgc caattcggca atggctcgcc gtccaatgcg attggcatcc
     5821 agcgaaactt tgatctctcg ccgcccgtca atatcgtggc ggtcgtgctc ggctattcat
     5881 ttgattactg aggactcctt gcaggcagca gctcgaagtg cagaacagga agcaaccgac
     5941 gacgcgccgg atctccggcg cgtcgacgat tacttcatcg gcgggtttca cgcgatggcc
     6001 agcccctgca cgctgctcat cgacaccgac gaccgcacag aagctggccg gtgtctcgcc
     6061 atcgcgagtg cggaagcgat ccggattgaa cggaaattca gccggtaccg ccgcgacaac
     6121 atcgtttacg acatcaacca cgccgaggga cggccggtcg tcgtcgatca tgagactgcc
     6181 ctgctgctca actatgcggc gacgtgttac gtcatatccg atggcatgtt cgacatcacc
     6241 tcgggcgtgc ttcgccgtgt gtggacgttc gacggaggat gcaaggtgcc gtcgcgtcag
     6301 tcggtagaag cttgtctgcg ccacctagga tggcagcgcg tcgcgtggaa aaacccggtt
     6361 ctgaccatcc cagtcggaat ggagatcgac ttcggcggaa tcggaaagga gtacgccgtc
     6421 gaccgggccg ccgctctgat cgcgtcgcgc acaaagagct cgttcgtcgt gaatttcgga
     6481 ggcgacctct acaccgcggg tctgcggcga gacggccgga catgggcaat cggcatcgac
     6541 gatcccggcc acaccggcaa gtccgccctt taccggctcg acgttccaaa gggcggcatt
     6601 gccacgagcg gcgacgctcg ccgtttcgtg caatggcgcg gcaagcgcct cggccacatc
     6661 ctcaacccga agaccggctg gcccgtcgag ggcgcccccc gatcgatcac ggtattcggc
     6721 cgcacgtgca tggaggctgg aacgttatcc acgctcgcct acctgcaagg aaggaacgcg
     6781 aaacagttcc tggagcagca aggcgtgcag ttctggatag tgtgagcgcg ggaatcggca
     6841 cgacgcgctg cgccgcggtc tgtagatttc ctttcggggt aggatggcgc taacggtcca
     6901 agaccagacc cgccactcgc gccgactaca agtcatcgcc gtcccaagag cgcatgtcgc
     6961 aaagctcgaa ctgatgcgtt cttgcacgca ttaagcgata aaagtcgcgc gtgtcaagag
     7021 tcgcaatctt tattcttctt cctaggcact ctcgagagag cttatccgca agaagcatga
     7081 gcatcgcatc aacgtagtca ataccgagat tggaacacag ctcattggaa cgcgacgaaa
     7141 ggtgctcgtc atctctaacc acggtaactg taccgggggt cgagacccat cgcagcattt
     7201 cgatcgcagg agctgcgctg cgcgctctac tattaacgat gccccacgcc tcgccgatca
     7261 cgcacaaagg aactaatatg gagggtacag tgtctagaaa gtagctagcc tcaatgtgaa
     7321 aatcgtcacg tggaaggaac cgcgcggaga ggatattcgt gtcgacgagg acggcgttcc
     7381 tgggaatgtc ggcgcgatac tcgtcaagaa cgtaagttgg cacgttctgt ctccggcaac
     7441 aatgagttaa cgatctcgtc tatcttcgcc tctgcatcag aacgagtgtc gggtggattt
     7501 gcgatgtgaa aaaaacttgc gtcgcgagcg aggcgagtga ggcagtcctg tatctcgatg
     7561 tcgaaaaacg ccctaggaat cggaatatca gtcaagtgct ggacttcgaa tttcttgtat
     7621 ccaccgcgga atgctggtgt tctttgatca agataccaac ccacaaagct gctattgaga
     7681 aatgcgagta acgcatcggc gatcacttcg tc
//
LOCUS       DATLVW010000158         7360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_5623, whole genome shotgun sequence.
ACCESSION   DATLVW010000158 DATLVW010000000
VERSION     DATLVW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5623"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1516
                     /locus_tag="VNN25_05150"
     CDS             365..1516
                     /locus_tag="VNN25_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXH90946.1"
                     /translation="MGNDILGIGLTGTSLTDLERRILRESTPFAVVLFGRNVGEAEEL
                     RELIREIKSLAPGAPPIFMIDEEGGRVDRLRHLIPGLPSAEAFAEGAEPAPMSQWCGR
                     VIGMALRWFDVEIDLAPVVDIRGDESPKGLERRTFGNDAEMVVELAGAFVRGLQGTGT
                     AACLKHFPGIGLGTGDPHYGETVMDLSLDELDRRDLVPFARLGNEAGGIMVGHGTYPQ
                     IDPSGSPASLSRILNRGLLDRIGFEGAAVSDDMEMHAVSDLGTYEEIADRALIAGNDV
                     ILFCSHIERVPDLQRHLRDKTKQDSVFAARFAEACKRAEGYRAHVARLRGNAPPPVAT
                     FDDVIDEAVRFVEKLELTRPHREIIIPDIDRRKGGRVPGKGRTGREEWT"
     gene            complement(1638..2807)
                     /locus_tag="VNN25_05155"
     CDS             complement(1638..2807)
                     /locus_tag="VNN25_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH90947.1"
                     /translation="MPRLKPQVRLLSFVSLLNDSASEMIYPLLPVFLTSVLGATPVTI
                     GVIEGAADGLASILKLVAGSVSDRLSRRKPLVVAGYALAAASRALIAVAGRWPTVLTA
                     RLIDRTGKGIRSAPRDAIIADVTPAADRGHAFGFQRALDHTGAVVGPLLALFFLNLLH
                     VPMRTLFMIAVIPGAIGVVLLLFLKEEPHTSTASEGSGLKASVPLPSRFWLAIAAVAL
                     FSLANSSDAFLILQAHASGVSTAMLPALWAAHHVIKSLFSTRAGALSDRVDRRLMLIA
                     GWCCYALIYVIFPFARSLWFFVALFVLYAIPFTLSEGAERAWISDLVPAEGRGKSFGI
                     YYLANGLCVLAGTILFGEIYQHIAPRAAFWTGAGLAVAAAAAVMIVPRRRSAQRS"
     gene            2929..3819
                     /locus_tag="VNN25_05160"
     CDS             2929..3819
                     /locus_tag="VNN25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXH90948.1"
                     /translation="MSEIKKVGVLGCGLMGSGIAQIAAQAGYETVVREVEQKFLDKGL
                     AGIDKSLSKFVEKGKLQAADKDASMSRLKGTTNLDDLADCDIVIEAIIENAELKKGTY
                     AALDKIVKKDALFASNTSSLTITELSMATARPKQFVGLHFFNPVPLMKLVEVVRTILT
                     SDESFTRAFQFAKSLGKEAVAARDNSGFIVNRLLVPYILDAIRAYEEGVGSIEDIDKA
                     MMLGCGYPMGPFTLLDFVGLDTTYFITEVMFAEYQEKRFAAPPLLRKMVLAGRLGRNK
                     TGSGFYDYASGTPVPFDKAK"
     gene            3868..4659
                     /locus_tag="VNN25_05165"
     CDS             3868..4659
                     /locus_tag="VNN25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXH90949.1"
                     /translation="MPYENLENVKAEVRDGILYVTIDRPKVLNALNAQTVEELYRIFA
                     EGRDDESVKAVILTGGGEKAFVAGADINELAQKTPITGKDTSERGQFILSFIERFPKI
                     VIVAINGFALGGGCELALACHIRIASEKAQIGLPEVTLGIIPGYGGTQRMARLLGKGK
                     ALELICTGDRIGAADAEKIGLVNRVVPADQLMATCEEMAKKIMSRGPLAVRAAIEAVN
                     HGSEMSFEEGQFLEASLFGLLCASADTKEGMQAFLEKRAANFQGK"
     gene            4953..5141
                     /locus_tag="VNN25_05170"
     CDS             4953..5141
                     /locus_tag="VNN25_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH90950.1"
                     /translation="MSSVDVIRRLRADGWTLVRTKGSHQHFKHPAKPGIVTVPHPRKD
                     MPFGTLKSIERQSGVKLT"
     gene            5138..5596
                     /locus_tag="VNN25_05175"
     CDS             5138..5596
                     /locus_tag="VNN25_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH90951.1"
                     /translation="MSTHEMDTALQYVAYVRKEGRRHLIEFPDAPGCQTFADREADIY
                     SNAKEAIEGWIEAHLVEGMDLPHTHRPPKVNERRVHITISPKLVLALQFRSMRSERGW
                     SQAEIAKRVGVSQQQIAKLEDPDANPTIETIMKVAHAFDMDLLVGFKKIA"
     gene            5714..6967
                     /locus_tag="VNN25_05180"
     CDS             5714..6967
                     /locus_tag="VNN25_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HXH90952.1"
                     /translation="MTENLQNLIYDWNAGNERPARPSPKKIEFDDETLRDGLQSPSVT
                     DPSIEEKIRILHFQNGIGADTSDIGLPGAGPHVQKSVERLAREIVDAKLNIAANCAAR
                     THENDIRPIAEISQRVGIPIEACTFIGSSPIRQFAEEWDLDWIIEQSTKAVRFAVGEG
                     LPVMYVTEDTTRAKPEDVEKLYTAAIEAGAKRVCVCDTVGHATPWGVRNLIRFVRGVV
                     DRANPDVKIDWHGHSDRGLGVINSIAAIEAGADRIHGSAAGIGERVGNTPIDLLMVNL
                     KLMEWIDNDLTTLPEYVRFVSEAVHIPLPDQYPVFGHDAFRTGTGVHAAAIIKAKKKG
                     SEWLADRVYSGVPAGMFGLEQIIEVGPMSGLSNVIYWLDSKGYPQDEALAKEIFQLAK
                     STNRVLTEEELHSCARRWQEVVAKG"
     gene            7040..7126
                     /locus_tag="VNN25_05185"
     tRNA            7040..7126
                     /locus_tag="VNN25_05185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7073..7075,aa:Leu,seq:caa)
BASE COUNT         1537 a         2233 c         2326 g         1264 t
ORIGIN      
        1 cccatcgagc gccttctccc cccgcgcttt ttgcgggggg agaaggtggc gaagccggat
       61 gagggggcct tccaatgagc acgccattcg tgcatatgcc gcgttttcag gtagttcggc
      121 cgaggcgttg ttgatcgtcc tgcgcacttg cttcccccct catccggctt cgccaccttc
      181 tccccccgca aaaagcgcgg ggggagaagg cgctcgatgg gaaggtactc ccgaggaaga
      241 ggggaaagac ccgaagaaag agcagaagcc ttcgcgcgtt cttgcttcgc atttgacaac
      301 gcgcgaagcg caatcaaccg acaaccgaca acgcgcgcag cgcaataagc tacgatcgca
      361 atccatgggc aacgacattc tcggcatcgg ccttaccgga acatcgctga cggacctgga
      421 gcggcgcatc ctgcgcgaat cgactccgtt cgccgtcgtt ctattcggac gcaacgtcgg
      481 ggaggccgag gagctgcgcg aactgattcg ggaaatcaag tcgctggcgc cgggcgcgcc
      541 gccgattttc atgatcgatg aggaaggtgg ccgcgtcgac cggttgcgcc atctcattcc
      601 cgggcttccc agcgcggagg cattcgcgga aggcgcggag ccggccccga tgtcgcagtg
      661 gtgcgggcgc gtgatcggca tggccttgcg ttggttcgac gtcgagatcg atctcgcgcc
      721 ggtggtcgac atccgcggcg acgaatcacc gaagggtctg gagcgccgca ccttcggcaa
      781 cgacgctgaa atggtcgtgg agcttgccgg tgcattcgtt cgcggactcc agggcacagg
      841 caccgccgcc tgcctcaaac attttcccgg catcggactc ggcaccggcg atccgcacta
      901 cggcgagacg gtgatggatc tctctttgga tgaactcgat cgccgtgatc tcgtcccctt
      961 cgcgcgactc ggcaacgaag caggcggcat catggtcggt cacggaacgt atccgcaaat
     1021 tgatccctcg ggcagcccgg cgtcgctgag ccgcatcctc aatcgcgggc tcctcgaccg
     1081 catcggcttc gagggcgccg ccgtcagcga cgatatggag atgcacgccg tctccgacct
     1141 cggcacctac gaagagatcg ccgatcgtgc cctgatcgcc ggcaacgatg tgattctctt
     1201 ttgcagtcac atcgagcgcg tccccgacct gcagcgccat cttcgcgaca agacgaagca
     1261 ggattccgta ttcgccgccc gcttcgcgga agcctgcaag cgtgcggagg gctaccgcgc
     1321 gcacgtcgca cgcctacggg gaaacgcgcc gccaccggta gcgacattcg atgacgtcat
     1381 cgacgaagcc gtccgcttcg tcgaaaagct cgagctgaca cggccacacc gcgagatcat
     1441 catccccgac atcgaccgcc gcaaaggtgg ccgcgtcccc ggcaaaggcc gcactggccg
     1501 cgaggagtgg acgtagcgcg ggaaagcgta gcgccgtcgg cccgacggct gtatcgccac
     1561 cggctcggtg gcgactttga tcgtgattcg aaaaacgccg ccgggccggc agcggtacag
     1621 ccgtcgagcc gacggcgcta cgaacgctgc gcagaacgac gccgcgggac gatcatcacc
     1681 gctgctgctg ccgccaccgc caatcccgcg cccgtccaga acgcagcgcg tggtgcgatg
     1741 tgctggtaga tctcgccgaa gaggatcgtg ccggcgagta cgcagagtcc gttggcgagg
     1801 tagtagatgc cgaagctctt gccgcgccct tccgccggca cgaggtcgct gatccaggcg
     1861 cgttccgccc cctcggacaa cgtaaacgga atcgcgtaga ggacgaagag cgcaacgaag
     1921 aaccagagcg atcgggcgaa ggggaagatc acgtagatga gcgcgtagca gcaccaacca
     1981 gcgatgagca taagacgccg gtcgacgcgg tccgacaatg cgccggcgcg cgtcgagaag
     2041 agcgacttga tcacgtgatg ggcggcccag agcgccggaa gcatcgccgt ggagaccccc
     2101 gaggcgtgcg cttgcaggat cagaaatgcg tcgctcgagt tcgcgaggga gaacagcgca
     2161 accgcggcga ttgcgagcca gaatcgggag gggagtggga cggatgcttt gaggccgctg
     2221 ccctccgaag ctgtggacgt gtgcggctcc tctttcagaa agagaagcag caccacgccg
     2281 atcgcgccgg ggatgaccgc gatcatgaag agcgtccgca tcggaacgtg gagaagattc
     2341 agaaaaaaga gcgcgaggag ggggcctacg acggcgccgg tgtggtcgag agcgcgctga
     2401 aaaccgaagg catgaccccg atccgcggcg ggtgtgacgt cggcgatgat ggcgtcgcgc
     2461 ggtgcggaac gaatcccttt gccggttcgg tcgatcaggc gggcagtcag cacggtcggc
     2521 catctgcccg ccaccgcgat caaagcgcgc gatgccgctg ccaacgcgta gccggcgacg
     2581 acgagcggtt tgcgtcgcga aagccggtcg gaaaccgacc ctgcgacgag cttgagaatc
     2641 gacgccagac cgtccgctgc tccttcgatc acgccgatcg tcacaggtgt cgcgccgagg
     2701 accgacgtca ggaacaccgg cagcagcgga tagatcatct cgctcgccga atcattcagg
     2761 agcgagacga acgagagcag gcgaacctgt ggtttgagac gcggcatcgc ttcaatgatg
     2821 aacgaggaag ggtgagacag gaaccgccat cacagtgcaa tgtgtttgcc gctcatcctc
     2881 gacggctcat cgttcgctta gaatgcgccg ctgcaaagga gcaggaccat gtcagagatc
     2941 aagaaagttg gcgtcctcgg ctgcggcctc atggggtcgg ggatcgctca gattgcggcg
     3001 caggctggct acgagaccgt cgtccgcgaa gtggaacaga agtttctcga caaagggctg
     3061 gccgggatcg acaagtcgct cagcaagttc gtcgagaagg gcaagctgca ggcggccgac
     3121 aaagatgcct caatgagccg gttgaaggga acgacgaacc tcgacgacct cgccgattgc
     3181 gacatcgtca tcgaggccat catcgagaat gcggagctga agaaagggac ctacgccgcg
     3241 ctcgacaaga tcgtcaagaa ggacgcgctc ttcgcatcca acacgtcgtc gctgaccatc
     3301 accgagctgt cgatggccac ggcccgtccg aagcagttcg tcgggcttca cttcttcaat
     3361 ccggtgccgc tcatgaaact cgtcgaggtc gtgcgcacga tcctgacgag cgacgagtcg
     3421 ttcacccgcg ccttccagtt cgccaaatcg ctcggcaagg aagcagtcgc ggcgcgcgac
     3481 aactccggct tcatcgtcaa ccgcctgctc gtgccgtaca tcctcgacgc tatccgtgcg
     3541 tacgaggagg gagtcggctc aatcgaggac atcgacaagg caatgatgct cgggtgcggc
     3601 tatccgatgg gtccgttcac gctgctggat tttgtcggcc tcgatacgac gtacttcatc
     3661 accgaggtga tgttcgccga gtatcaggaa aagcgcttcg ccgcgccgcc gctgctccgc
     3721 aagatggtgc tcgcgggccg tctcgggcgg aacaagaccg gaagcggttt ctacgactac
     3781 gcgagcggca cgccggtgcc attcgacaaa gcgaaatagt ttttcatctg cgttccatct
     3841 gcgtcatctg cggataaagg acactccatg ccttacgaga atctcgaaaa cgtcaaagcc
     3901 gaagtgcgcg acggcatcct ctacgtcacg atcgaccggc ccaaagtgct caacgccctg
     3961 aacgcgcaga cggtcgaaga gctgtaccgc atctttgccg aggggcggga cgatgagagc
     4021 gtcaaagcgg tgatcctcac aggcggcggg gagaaagcct tcgtcgccgg cgccgacatc
     4081 aacgaactgg cgcagaagac tcccatcacc ggcaaggaca cctcggagcg cggacagttc
     4141 atcctctcgt tcatcgagcg cttcccgaag atcgtcatcg tggcgatcaa cggcttcgca
     4201 ctcggcggtg gctgcgagct cgcccttgcc tgccacatcc gcattgcctc ggagaaagcg
     4261 cagatcgggt tgcccgaggt gacgctgggc atcattccgg ggtacggcgg aacgcagcgt
     4321 atggcgcgcc tgctcgggaa gggcaaagcg ctggaattga tctgcaccgg cgaccgcatc
     4381 ggcgccgcgg acgccgagaa gatcggcctc gtcaatcgcg tcgtccccgc cgaccagctc
     4441 atggccacgt gcgaggagat ggcgaagaag atcatgtcgc gtgggccgct ggccgttcgt
     4501 gccgcgatcg aagcagtcaa tcacggcagt gagatgtcat tcgaagaggg ccagttcctc
     4561 gaggcgtcgc tcttcggcct gctctgcgcg agcgcggaca cgaaggaagg gatgcaggcg
     4621 tttctggaga agagggcggc gaattttcag gggaagtgag atcggatatg gccgaggcgc
     4681 gaagcacctg aagatctggt acgactccga aggcgactac gtcgaagtga tcttcgatca
     4741 gaagacaggg tacttccgcg aaaccgagag tgaccacgtc atgaagaagg tcgacgacga
     4801 agggaacgtg cttggttttt ccgtgctcgg cctccgtgcg atgaagcaga acccgctcga
     4861 cgtcgcgctt tgagcatgat tgatcctggg gtcgggggat tggcgagaaa gatcttcttg
     4921 gattacaacc taaaagttgt ataattcaat gtgtgtcctc cgtagacgta atccgaagac
     4981 tccgcgcgga cggatggacg ctggtgcgca cgaagggttc gcaccagcat ttcaagcacc
     5041 cagcgaagcc ggggatcgtc acggtgcctc acccgcggaa ggacatgccg ttcggcacgc
     5101 taaagagcat cgagcgtcaa tcgggagtga agctgacatg agcactcatg agatggatac
     5161 cgcgctgcag tacgttgcct atgtgcgtaa ggaggggcgc cggcatctga tcgagttccc
     5221 tgacgcccca ggctgtcaga ccttcgcgga tcgcgaggca gacatctact cgaatgccaa
     5281 ggaggcgatc gagggatgga tcgaggccca tctcgtggaa gggatggact tgccgcatac
     5341 gcaccggccg ccgaaggtaa atgaacggcg cgtccacatc acgatttctc ccaagctcgt
     5401 tcttgccttg cagttccgat ccatgcgaag tgagcgcggc tggagccagg ccgaaatcgc
     5461 aaagcgcgtc ggtgtctcgc aacagcagat cgccaagctc gaagatcctg atgcaaatcc
     5521 tacaattgag acgatcatga aggttgccca tgcgttcgac atggacctcc tcgtcggttt
     5581 caagaagatc gcctgagcga agcggtggcg aggcgaccgg cggaccgtca gctttaaggc
     5641 gtcgtaagcc gtccacttgc cggtgaacga caggtcagcg aaagtcgtcc acagttgcta
     5701 gtattcgtac tcgatgaccg agaacctcca gaacctcatc tacgactgga acgcgggcaa
     5761 cgagcgtcct gcgcggccgt cgccgaagaa gatcgagttc gatgacgaga cattgcgcga
     5821 cggactccag tcgccctcgg tgaccgatcc gtcgatcgag gagaagatca ggattctgca
     5881 cttccagaac ggcatcggcg ccgacacctc ggacatcgga ctgcctggtg ccgggccgca
     5941 cgtccagaaa tcggtcgagc ggctggcgcg cgagatcgtc gatgcgaagc tcaacatcgc
     6001 cgccaactgc gcggcgcgga cgcatgagaa cgatatccgg ccgatcgccg agatctcgca
     6061 gcgtgtcggc atccccatcg aggcctgcac cttcatcggc tcctcgccca tccggcaatt
     6121 cgcggaggag tgggatctcg actggatcat tgagcaatcg acgaaggcgg ttcgctttgc
     6181 cgtcggcgaa gggctgccgg tcatgtacgt caccgaagac acgacccgcg caaaaccgga
     6241 ggacgtcgag aagctctaca ccgccgcgat cgaagctggt gcgaagcgcg tctgcgtctg
     6301 cgatacggtt ggccacgcca cgccgtgggg agttcgcaac ctcatccgat tcgtccgcgg
     6361 cgtcgtcgac cgcgccaatc ctgacgtgaa gatcgactgg cacggccaca gcgaccgcgg
     6421 cctcggcgtc atcaactcca tcgcagcgat tgaagccggc gccgaccgca tccacggctc
     6481 agcggcaggc attggcgagc gcgtcggcaa cacgccgatc gatctgctga tggtcaatct
     6541 caaactgatg gagtggatcg acaacgacct gacgacgctg cccgagtacg tgcgcttcgt
     6601 ctcggaagcc gtgcacattc cgctgcctga tcagtatccc gttttcggcc acgacgcctt
     6661 ccgcaccggc accggcgtcc acgcagcggc gatcatcaaa gctaagaaaa agggaagcga
     6721 gtggcttgcc gaccgcgttt actcaggcgt tcccgcagga atgttcggtc tcgagcagat
     6781 cattgaagtt ggcccgatgt ccggcttgtc gaacgtgatc tactggctcg actcgaaagg
     6841 ctatccgcag gacgaagcgc tggcgaaaga gatcttccaa ctggcgaagt cgacgaaccg
     6901 ggtcctgacg gaggaagagc tgcacagttg cgcgagacgg tggcaggagg tcgtcgcgaa
     6961 aggatgaaga cggaaggatg acggatgaag ttgctttgga attcatcctt catccttcag
     7021 gcttcatcca ttgatcctgg cccgagtggc gaaatggcag acgcagcaga ctcaaaatct
     7081 gccgaggtta accctcatgt cggttcgagt ccgacctcgg gcaccaattg atgtgaacag
     7141 atcgtgacgc cttgcctcgt ggcaatttcg tcgcgacgct cgcgagtttc gcggcgtaga
     7201 gcagttaacg gagactaata actcgcgcat gcgccgccac ctgatcgcat ctctcgtcct
     7261 tctcatcctt ccgattgctg tctacgcact gcctcgcgat ggcgggacaa tgcgtcgcca
     7321 ttacattctg gagagcaccg ttccgctcga tgcggctgcc
//
LOCUS       DATLVW010000159        12008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_377694, whole genome shotgun sequence.
ACCESSION   DATLVW010000159 DATLVW010000000
VERSION     DATLVW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377694"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..804
                     /gene="msrA"
                     /locus_tag="VNN25_05190"
     CDS             214..804
                     /gene="msrA"
                     /locus_tag="VNN25_05190"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXH90953.1"
                     /translation="MKRILLSLAALILLFAAAPQPRRATATFAGGCFWCMEPPFERLH
                     GVLSVTSGYTGGRVDKPTYEQVSDGFTGHREAVEVVYDPAQISYQQLLDVYWYNVDPF
                     DQSGQFCDKGGQYRAAIFVHDAGQRRLAEASKQAVGVRFHRDVSVDIVTAGTFYRAEE
                     YHQHYSKKNPLRYKFYRFNCGRDQRLKEVWGAAPEH"
     gene            complement(922..2361)
                     /locus_tag="VNN25_05195"
     CDS             complement(922..2361)
                     /locus_tag="VNN25_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXH90954.1"
                     /translation="MARPRLPKSTIECPDCTKASGFPLGQSEGFSRRRFMQIAGTGLV
                     ASYFADVLSPRLLQGATTAGGVYLRATAKNAIFIFLPGAPSNIDTWDLKEGAWTPSDF
                     NPTSYDNNNVRFPQGLMPMTAAQLDKMTFVRSGLSWAAVHTLGQAWVQIARNPGGATG
                     SIAPHIGAVVALESQINRKPTDVLPGFIALNSTGIPASGYFPASYAPFQVVPAATGLP
                     TIAHPDGASRFSDRWSFMQQLDSARTTGALGKATLDMNDFYTQSKALMDAPNINAVFS
                     FSTAEHTKYGATTFGDSLIVARNIVAAGKGTRFVQATLGGWDHHSGIYTKPANGVNSL
                     YTQCAQFDPAYASLLADLSTMPGSVSGKTLLDETLVVIVAEFGRTVGVLNNQAGRDHN
                     LRMTTVWAGGGIRGGNVIGKTDVTGGSVADFGWSGNRDIRPEDVVSTIYSALGIDYTT
                     MRHDDPLNRGFEYVPFAKDGTYKPIDEMF"
     gene            complement(2374..4215)
                     /locus_tag="VNN25_05200"
     CDS             complement(2374..4215)
                     /locus_tag="VNN25_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HXH90955.1"
                     /translation="MKQWIALAVVAPTLLLGVEKETCPFGPFDLRSPREQQASLNHQL
                     SAVAEAVAPSGRRRSATPPAGDVSYPVSVNYIDDEIFGKMKTDGIRPAPMSSDEEFLR
                     RVTIDLTGQIPDPATVLAFLADKSADKRAKTIDALLASDAFNDRWTMWFGDLVQNVQV
                     SANSREFPQGRNAYYTYIHDSIKANKPYDQMVRELLGGMGDSFLAGPPNYWVRQIQPN
                     GPIQDTYDNLAAESGRMFLGMPMQCISCHNGLGHLDQVNLYLKGHARYDFWGNASFFS
                     RGRFAKSDKNVNNSSYVITEATTGSYQLNTTSGNKTARAPIGTQTTVSPSFLMTGETP
                     RAGEAWRDAYGRMLTANPQFSLAAVNYLWQQMFGLAIVEPANNMDPTRYDPNNLPAGQ
                     TVQPNHPALLTRLGSDFTANHYDLRAILRTMAMSNAYQLSTQYTASTWNESWTPYYAR
                     HIPRRLMAEALLDAVAKSTSAPITYTVSGGLPAVTSAMKLPDTLEVRGNVYGRFLDEF
                     GRGNRDDEPRENDSSIAQALSLMNDGTVVVNRTHRATANSTVAKVLASTTDPNSIADQ
                     LYLNTLSRYPTASEKAQAVAYLKSGTLANKTEDLQWVLLNSLEFLFD"
     gene            complement(4467..4988)
                     /locus_tag="VNN25_05205"
     CDS             complement(4467..4988)
                     /locus_tag="VNN25_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH90956.1"
                     /translation="MFRLETDRLYIRPWTPADRPAFTGLTEDPEVMRYVHGGQPYAED
                     EVDEWFTRQARQLAEHDVCMGALIEKATGRLVGIAGTQPLGTTGNFEIGWWLARDVWG
                     RGYATEAGGAAMRHVLETLGHKRVVAIIDPDNEPSKRVVGRLGMHYESRVTGEQLGHR
                     KPQIVVDLFAKEA"
     gene            5808..8468
                     /locus_tag="VNN25_05210"
     CDS             5808..8468
                     /locus_tag="VNN25_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH90957.1"
                     /translation="MEEPLDLSGRELGRYHLLSLLGSGGMGAVYEAIDETLGRHVALK
                     ILPPELVGDADRVRRFSQEARAASALNHPHVITVYDIGTHEVDGKVLHFIAMEKLDGR
                     TLRETLRDGPLPAENAVEIMLQVTDGVAAAHASGIVHRDLKPENVMITTDGYAKVLDF
                     GLAKLQEDPSQTSDTSASTDVMLTGAGTILGTVGYMAPEQAAGRRADHRADIFSIGCI
                     LYEVVSGRRAFRGESTLDTLHRIVHDDPEPLSSLVPGTPHELPRIVGKALAKDPDKRY
                     QSARELAVDLRVLLRQVHSDDAGRAIVQEKRRSWTLPIVVIALLVLIASAAIWAALRW
                     QYRTGSAEGGRLEIKRVTSSGDVISAVISPDGKYIAYCVSNSSGQSLWVRQLATRQSL
                     QIIPPIQHAAVYGQTFTPDGNAIVYVLVGSNDPNGALYRVSTLGGQPERLLEGIEDSP
                     SFSPDGRRITYLRRDFPRPGESALMVAGSDGTGARMIATRRYPELFAPIFFGGPSWSP
                     DGSIIATAVARAEHPWAANLFAFDPEHGTAKPLSDGNWRALAQAAWLPDMTGLVVVGT
                     RQDDPFASAGLALRLWFVPYPSGAPRQITRDLLFYRAATISADGRSLLTVGNDVSCQL
                     WRVPLGHSEQRQKISTGKMDGWEGLSLAPDGRIIFSAVEETDINLVIANADGGDSKPL
                     TRPPGAAMFPAAFSNGFAFIAPGPSGNEVRATGYDLQGTRTIVRGVDGHPIDVSRDGQ
                     WLAYRVDRRLWKIRMDGTGAKQLTSSPVTFASWSPSGDRLAILYNDGHDRLGILPAAG
                     GATTWSIPVSLHSHSIVRWSPDGNSLLVNDFQNDHADIWRIPFTGPPQKLTTLEEHDA
                     YFFDVLPDGKTAIVCRGPLSRDAVLITGFH"
     gene            8542..8796
                     /locus_tag="VNN25_05215"
     CDS             8542..8796
                     /locus_tag="VNN25_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HXH90958.1"
                     /translation="MATKKIQAAMLSLALLLPAGSTFASTYHHHYRHYQHHYSQTRGA
                     VIGAVAGAAIDHHQPLKGALIGGAVGEGVQMLRNHHKRHY"
     gene            8828..10291
                     /locus_tag="VNN25_05220"
     CDS             8828..10291
                     /locus_tag="VNN25_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH90959.1"
                     /translation="MKRLTILLLFLATTALGQKPDQHAVIAEFIDLLAIPNLASDTPN
                     IARNAAAVQAMFEKRGVPTRLLALDGAPPIVIGDLRVPGATRTIAFYAHYDGQPVDAT
                     QWKSAPWTPVMRDAAGNDIDWRSAKELNPEWRIYARSAGDDKTPIVGMAAALDLLRAA
                     GHKPAVNLRFVFEGEEEAGSPHLAAYLAKYPDVLRPDAWILCDGPVHQSRRMQLVFGA
                     RGVVDLELTVYGPVKGLHDGHYGNWVPNPIVRLTHLIDSMRDESGRILIAHFYDDVRP
                     SSKAEQDAIAKMPPVESDLKHEFAIAETEGNGRPLNELLMLPALNVRGIQSGHVGAQA
                     SNTIQTEAVASLDFRLVPAETPESVKALVEHHLETLGYTIVRTTPDLAVRAAHAKLVK
                     AVWGSGYPPARTPLDLPFSNQIATLMTTAGHDPVRVPTLGGSIPMYLFQQPANTPVLV
                     LPIANHDDNQHAANENIRLQNLWDGIDVYVALFEGLK"
     gene            10423..11058
                     /locus_tag="VNN25_05225"
     CDS             10423..11058
                     /locus_tag="VNN25_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90960.1"
                     /translation="MRERFGVILFSLFFASVAAAQLSRNPCINFPCPALQSCISPADA
                     PSCVADSPTRCSVVTVYTHGLMDSFAQPVDPTTPSPTLTTFLGAAISRYDTASCNQHF
                     GDSFPVGSCLNCKFCGAKVEIHLKPCGSDQPGNDDYVIGVAPFGTTQILASGRIWPTG
                     VTPREEILVVNIPADKLNQLFCNVRVRPPTLDVYVEDDTIVDWVRVTIYSS"
     gene            11165..11761
                     /locus_tag="VNN25_05230"
     CDS             11165..11761
                     /locus_tag="VNN25_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXH90961.1"
                     /translation="MSKARLETFTDGVVAILITIMVLELHVPHGTAWSALGPLIPSLL
                     TYALSFIFLAIYWNNHHHMFHAVNRVNGRILWANMHLLFWLSLVPFITGWMGENNFAS
                     IPLAAYGVVLLMAAIAYTILQFAIIADQGPHSPLAAAVGDDVKGKISAALYAIAIPLS
                     FYQRWIAGAIYLTVAMIWLAPDRRIEKKVESGVLPHEK"
BASE COUNT         2211 a         3767 c         3694 g         2336 t
ORIGIN      
        1 tagccgcaac caaatgaaac ccaccaaatc tgcaaccaat cagttttggt gaaaatcttt
       61 gcgtcgcgcg cagaaaccgc tggttagtag tacagagaac acagagggga tctctgtgac
      121 ctctgtgcct ctgtggtgac cgggttttca aattgaccac catccaacat ttaactggat
      181 tcccaaggac gctctgtacg aatgcccaac cacatgaaac gcatcctcct ctccctcgcc
      241 gcgctgatcc tcctcttcgc tgccgcgccg caaccacgcc gcgcgacggc cacattcgct
      301 ggcggttgct tctggtgcat ggaaccgccg ttcgagcggt tgcatggcgt cctctccgtc
      361 acctcggggt acacaggcgg acgcgttgac aagccgacgt acgagcaggt ctcagacggt
      421 ttcacaggac atcgcgaggc cgtcgaagtc gtctacgacc ccgcgcagat ctcgtatcag
      481 caactactcg atgtctactg gtacaacgtc gatccattcg atcaatcggg gcagttttgc
      541 gacaaaggag gacagtatcg cgcggcgatc ttcgttcacg atgccgggca gcgacgtttg
      601 gccgaggcat cgaaacaggc cgtcggcgtg cgatttcacc gcgatgtctc cgtggacatc
      661 gtgaccgcgg gaacgttcta tcgtgccgag gagtatcacc agcactactc caagaagaac
      721 ccgcttcgct ataagttcta tcggttcaac tgcgggcggg atcagcggct gaaggaagtg
      781 tggggcgcgg cgccggagca ttgaagttgg gggagtgggg tgtggggtgt ggggtgtggg
      841 agcaaaaacg caagcaaaac agagcacaga gactcagttt ttttctccca ccccccactc
      901 cctacacccc actccccgca actagaacat ttcgtcgatc ggcttgtacg ttccgtcttt
      961 cgcgaagggg acgtactcga agccgcggtt gaggggatcg tcgtggcgca tcgtggtgta
     1021 gtcgatgccg agggcggagt agatcgtcga gacaacgtct tccgggcgga tgtcgcggtt
     1081 gccgctccat ccgaaatcag ccacgctgcc gccggtgacg tcggtcttcc cgatcacatt
     1141 gccgccgcga atgccgccgc ccgcccacac cgtcgtcatg cggagattgt gatcgcggcc
     1201 ggcttgattg ttgaggacac cgacggtgcg gccgaactcg gccacgatga cgacgagcgt
     1261 ctcatcgaga agcgtttttc cggagactga gccaggcatc gtggacaggt cggccagtag
     1321 cgatgcgtac gccggatcga actgcgcgca ctgcgtgtag agcgaattca cgccgttcgc
     1381 aggcttcgtg tagatgccgc tgtggtgatc ccatccgccg agggtggcct gaacgaagcg
     1441 ggttcccttg cctgccgcga cgatgttgcg cgccacgatc agcgaatcgc cgaaggtcgt
     1501 cgcaccgtac ttggtgtgct cggcagtcga gaagctgaag acggcgttga tgttgggcgc
     1561 atccatcaag gctttcgact gcgtgtagaa gtcattcatg tccagcgtgg ctttaccgag
     1621 cgcgcctgtg gtgcgggcgg agtcgagttg ctgcatgaac gaccagcggt cggagaagcg
     1681 tgaggctcca tcgggatgcg cgatggtcgg caagccggtt gcggccggca cgacctggaa
     1741 cggcgcatac gatgccggga agtagccgga ggccgggatg ccggtggagt tgagggcgat
     1801 gaagcccggc agaacgtcag tcggcttgcg attgatttgc gactccagcg cgacgaccgc
     1861 accgatatgc ggagcgatcg atccggtcgc gccacccgga ttccgcgcga tctgcaccca
     1921 cgcctgtccc agcgtgtgca ccgccgccca ggagagaccg gaacgaacga acgtcatctt
     1981 gtcgagctgc gcggccgtca tcggcatcag cccctgcggg aagcgcacgt tgttgttgtc
     2041 gtacgacgtg gggttgaagt cgctaggcgt ccatgcccct tccttcaggt cccacgtgtc
     2101 gatgttgctc ggcgcgccgg ggaggaagat gaagatggcg ttcttcgcgg tggcgcgcag
     2161 atagacaccg ccggccgtgg tcgcgccctg cagcaggcgc gggctgagaa cgtcggcgaa
     2221 gtacgaggcc acgaggccgg tgccggcaat ctgcatgaag cggcggcggg agaagccctc
     2281 actctggccg agcggaaaac cgctggcctt cgtgcagtcg gggcactcga tcgtgctctt
     2341 cggaagtctc ggtcttgcca tggtcatcag tcctcaatca aacagaaact cgagcgagtt
     2401 gagtagcacc cactgcagat cttcggtctt gttggcgagt gttccgctct tcagataggc
     2461 aacggcctgc gccttctcgc tcgcggtggg gtagcgcgaa agcgtattca gatagagctg
     2521 atccgcgatg ctgttgggat ctgtggtcga agccagcacc tttgctaccg tcgaattcgc
     2581 cgtagcccga tgtgtacgat tcacgaccac ggtgccgtca ttcatcaacg agagagcctg
     2641 ggcaatcgag gagtcgttct cgcgaggctc atcatcgcgg ttgccacggc caaattcgtc
     2701 gagaaagcgt ccgtaaacat taccgcgcac ttcgagcgtg tcggggagtt tcatggccga
     2761 cgtgaccgcc ggcaggccgc cgcttaccgt gtacgtaatc ggtgcgctgg ttgacttcgc
     2821 cacggcgtcg agcagcgctt cggccatcaa gcgccgcgga atgtgacgcg cgtagtacgg
     2881 cgtccacgat tcgttccacg tgctggcggt gtactgcgtc gaaagctggt aggcattcga
     2941 catcgccatc gtgcgcagaa tcgcgcgcag gtcgtaatga ttggcggtga agtcgctgcc
     3001 gaggcgggtc aggagcgcgg ggtgattcgg ctggacggtt tgtcccgccg ggaggttgtt
     3061 cgggtcgtag cgcgtcgggt ccatgttgtt ggccggctcg acgatggcga ggccgaacat
     3121 ctgttgccag aggtagttca cggccgcgag cgagaactgc gggttggcgg tgagcatgcg
     3181 tccgtaggca tcgcgccagg cttcacccgc acgcggcgtc tcgccggtca tgagaaacga
     3241 cggcgaaacg gtggtctgcg ttccgatcgg agcgcgtgcg gtcttgtttc ccgacgtcgt
     3301 attcaactga taggagccgg tcgtcgcttc ggtgatgacg tagctggagt tgttgacgtt
     3361 cttgtcggac ttcgcgaagc ggccgcgcga gaagaacgaa gcgttgcccc agaagtcgta
     3421 acgtgcatgt cccttcagat agaggttgac ctgatcgaga tggccgaggc cgttgtggca
     3481 ggagatgcac tgcatcggca tgccgagaaa catccggcct gattcggcgg cgaggttgtc
     3541 gtacgtgtcc tgaatcggac cgttgggttg gatctgccgc acccagtagt tcggcggccc
     3601 cgccaggaag ctgtcgccca tgccgccgag tagctcgcgc accatctggt cgtagggctt
     3661 gttcgctttg atcgaatcgt ggatgtaggt gtaataggcg ttgcgtccct gcgggaactc
     3721 gcggctgttc gcggagacct ggacgttctg tacgagatcg ccgaaccaca tcgtccagcg
     3781 gtcgttgaag gcgtcggagg caaggagcgc atcgatcgtc ttggcccgct tgtccgcgga
     3841 cttgtccgcg agaaaagcca gcaccgtggc cggatcggga atctggccgg tgaggtcgat
     3901 ggtcacgcga cggaggaact cttcgtcgga cgacatcgga gccggccgga tgccgtcagt
     3961 cttcatcttg ccgaagatct cgtcgtcgat gtagttgacc gacaccgggt acgagacgtc
     4021 gccggcagga ggagtggccg agcgacggcg tccgctcggt gccaccgcct cggcaaccgc
     4081 cgagagctgg tggttcaggc tggcctgctg ctcgcgcggc gagcggaggt caaacggacc
     4141 gaacgggcac gtttcctttt caacgccgag aaggagtgtc ggcgcgacaa ccgcgagagc
     4201 aatccattgt ttcatgtcgc tttggactcc tgtcacatag gaatgtttac atgctacgcc
     4261 cgaatgggaa ggagggcgta gttcatgcca agtgggtgcc cacgcgaaga cgcgaagtcg
     4321 cgaaggtgtt caaaaggctc cttgacgaag agatcgacct gcggcttgcg gcgacccgga
     4381 tccagtacgg agggcatcgt cacatgccgg gcgggtaccc caacctgcct ctctccttcg
     4441 cgtcttcgcg tcttcgcgtg accggtttac gcttccttcg cgaagaggtc gaccacgatc
     4501 tgcggcttgc ggtggcccag ttgctcgccg gtgacgcggg actcgtagtg catgccgaga
     4561 cggccgacga cacgcttgga gggctcgttg tcggggtcga tgatggcaac gacgcgcttg
     4621 tggccaagcg tttcgagcac atggcgcatg gcggcaccgc cggcttccgt ggcatagccg
     4681 cggccccaga cgtcgcgggc cagccaccag ccgatctcga agttgccggt cgtgccgagg
     4741 ggctgggttc cggcgatgcc gaccaggcgg ccggtagcct tttcgatcag cgctcccatg
     4801 cagacgtcgt gctccgccaa ctggcgtgcc tgacgcgtga accattcatc gacttcgtcc
     4861 tccgcatacg gctgcccgcc atggacatac cgcatgacct cggggtcttc ggtgagtccg
     4921 gtgaaagccg gccggtcggc cggcgtccac ggccggatgt agagacgatc cgtctcgagt
     4981 cgaaacatgt gtgcccgatc gaaccgctac ttcttgtctt tcttgggctt cttcgtttct
     5041 ttcttccgag cgttgtttcc tttggccatg acgtcctcct aatgtttgtt gctgatcaag
     5101 gttagcggat tttctggttt tgggaagtgg ggggtggggt atgggggtgg cagcgggtgc
     5161 gcgagacccg aggctgggtc gctggcgacg tgcggacccg ccgcgcgaaa cccgcggggt
     5221 cggggtgtgg acccgagggt tttccatgca gacccttggg ttatccgcgc gaccccgagg
     5281 gttttccatg cgaaccctgg gttatcccgt gcgaccccga gggttttcca tgcagaccct
     5341 tgggttatcc gtgcgacccc gagggttttc catgcgaacc cttgggttat ccctgtgtac
     5401 cccgagggtt taccgtgcaa ccccgcgggt ttaccgtgcg acccccaggg ttttccatgc
     5461 aaacccttgg gttatccgtg cgtaccccga gggtttacgg tgcaaccctg agggtttacc
     5521 gtgcaacccc gagggtttac cgtgcaaccc cgagggtcta ccgtgcaacc ccgagggtct
     5581 accgtgcaac ctcgagggtt taccgtgcaa ccccgagggt ctaccgtgca accccgaggg
     5641 tttaccgtgc aaccccgagg gttgccgtgc aacccccagg ttttaccgcg cggacccgag
     5701 gcctcgtcgc gattctgagg tttatcccac aacccacccc ccactcccca ccccccagga
     5761 gcagccgagc cttcacacaa actgattgac ggcgttactc tttacaaatg gaggagccgc
     5821 ttgacctctc gggccgcgag cttggacgct accatctcct gtcgctcctc ggatcgggtg
     5881 gaatgggtgc ggtgtacgag gccatcgacg agaccctcgg acgtcatgtc gccctgaaga
     5941 tcctgccgcc ggagctggtc ggcgacgcgg atcgagtccg gcgtttttcg caggaagcgc
     6001 gcgcggcatc cgcccttaat catccgcacg tcatcacggt ctacgacatc ggcacgcacg
     6061 aagtcgacgg aaaggtactg catttcattg caatggagaa gctcgacggc cgaacgttgc
     6121 gcgagactct cagagacggt ccgctgccgg ccgagaacgc ggtcgagatc atgttgcagg
     6181 tgaccgatgg agtggcggca gcgcacgcat caggcatcgt tcatcgcgat ctcaaaccgg
     6241 agaacgtgat gatcaccacc gacggctacg cgaaagtgct cgacttcggt ttggccaaac
     6301 tgcaggaaga tccgtcgcag acgagcgaca cgagcgcctc taccgacgtg atgctgaccg
     6361 gcgccggcac aatcctcggc accgtcggct acatggcgcc ggagcaagcc gccgggcggc
     6421 gcgcagatca ccgggccgac atcttctcga ttggttgcat cctttacgaa gtcgtcagcg
     6481 gacgtcgtgc gttccgcggc gagtcgacgc tggatacgct gcatcgcatc gtccacgacg
     6541 atccagagcc gctatcgtcg ctcgttcctg gcacgccgca cgagcttccg cgcatcgtcg
     6601 gaaaagcact ggcgaaagat ccggacaagc gctaccaatc ggcgcgcgag ctggccgtcg
     6661 atctgcgcgt tctgctccgg caagtgcact cggacgatgc gggccgcgca atcgttcagg
     6721 aaaaacgacg ctcgtggacg ttgccgatcg tcgtcattgc cctgctggtt ctcatcgcgt
     6781 cagcagcgat ctgggctgcg cttcggtggc agtatcgaac gggaagcgcc gagggaggcc
     6841 gtctggagat caagcgcgtc acgtcgagcg gcgacgtgat ttccgcggtc atttcgccgg
     6901 acggcaagta catcgcgtac tgcgtatcga actcctcggg acaaagcctc tgggtccggc
     6961 aactggccac gcggcaaagc ctccagatca ttccgccgat ccagcacgcc gccgtgtacg
     7021 ggcagacctt cactcccgac ggaaacgcga tcgtctacgt gctcgtgggc agcaacgacc
     7081 cgaacggggc gctctatcga gtgtcgacgc ttggcggcca gccagagcgg cttctcgaag
     7141 gcatcgagga ctcgccttcg ttttcgccgg acgggcgccg gataacttac ctgcggcgcg
     7201 acttcccgcg gccgggcgag agcgcactca tggtggcggg cagcgacggc acgggcgcac
     7261 gcatgatcgc taccaggcgg tatcccgagc tcttcgcccc gatcttcttc ggaggtccga
     7321 gctggtcacc cgacggatcg ataatcgcga cggccgtcgc acgcgccgag catccctggg
     7381 ccgcgaatct gttcgcattc gatcccgagc acggaaccgc gaaaccgctg agcgatggaa
     7441 attggcgcgc tctcgcacaa gctgcgtggc tgcctgacat gacgggtctc gtggtcgttg
     7501 gcacacgtca ggacgatcct tttgcgtccg cgggattggc cctgcggcta tggttcgttc
     7561 cctatccgtc gggcgctccg cgtcagatca ccagggatct gctgttttac cgcgcggcca
     7621 cgatctcggc agatggccgc tcgctcctga ccgtggggaa cgacgtgtcc tgtcagttgt
     7681 ggcgcgttcc gttgggacac agcgaacagc gtcagaagat ctcgacagga aaaatggatg
     7741 gatgggaggg cttgtcgctg gcacccgacg gccgcatcat cttcagcgcc gtcgaagaaa
     7801 ctgacatcaa tctcgtcatc gcaaatgccg acggcggaga ttcaaagcca cttacccggc
     7861 ctcccggtgc agcgatgttt cccgccgcgt tctccaacgg attcgccttc atcgcacccg
     7921 gcccttcggg caacgaggtt cgcgccacag gctacgacct ccaaggaaca cggacgatcg
     7981 tgcgcggagt cgacggccat ccgatcgacg tttcgcgcga cggccaatgg ctggcctaca
     8041 gagtcgatcg ccgactctgg aagattcgca tggacggtac aggcgcgaag cagttgacgt
     8101 ccagtcccgt cacctttgcg tcgtggtctc caagcggtga ccggctcgcc atcctctaca
     8161 acgacggaca cgaccgcctc ggcatcctgc cggctgcggg cggtgcgacg acgtggagta
     8221 ttccagtctc tctccactct cacagcatcg ttcggtggag ccctgacggt aattctctgc
     8281 tcgtcaacga ttttcagaac gatcacgccg acatctggcg gattccgttc actggtccgc
     8341 cacagaagct cactaccctc gaggaacacg acgcgtattt cttcgatgtt cttccggacg
     8401 ggaaaacggc gatcgtttgc cgcggcccgc tgagccggga cgcggttctg atcaccggtt
     8461 ttcactgatc taaatctctt caactgttga gtttaagcgg caccgatctc gcactagtac
     8521 aggccaggag gaacacccca aatggccacc aaaaagatcc aggcagcgat gttgagcttg
     8581 gcgctccttc tgcctgccgg aagcacgttc gcctcgacgt atcatcacca ctatcgccat
     8641 taccagcacc actacagcca gacccgaggg gcagtcatcg gagccgtcgc cggtgcggca
     8701 atcgatcacc atcagccgct gaagggtgcg ctgatcggtg gcgcggtcgg cgaaggcgtc
     8761 cagatgctgc gcaaccacca caaacgtcac tactagcaga accggaaatc acgtatggtg
     8821 aggcgcgatg aaacgcctca ccatccttct cctttttctc gcgaccacag ctctcggcca
     8881 gaagcccgac cagcacgccg tcatcgccga gttcatcgat cttctggcga ttcccaacct
     8941 cgcctccgat acgcccaaca tcgcgcgcaa cgccgccgcg gttcaggcca tgttcgagaa
     9001 acgcggcgta ccgacgcggc tgctcgcgct cgacggcgcc ccgccgatcg tcatcggcga
     9061 ccttcgcgtg ccgggcgcga cgcgcaccat cgcgttctat gcgcactacg acggccaacc
     9121 agtcgatgcc acgcagtgga aaagcgcgcc gtggactccc gtcatgcgcg atgccgcggg
     9181 taacgatatc gattggcgga gtgcaaaaga gctgaacccc gagtggcgga tctacgcgcg
     9241 ctccgcgggc gacgacaaga ctccgatcgt cggaatggcc gccgcactcg atcttctgcg
     9301 cgccgcgggt cacaaaccgg cggtcaatct ccgtttcgtt ttcgagggcg aagaggaagc
     9361 gggctcacca cacctggccg cgtatctggc gaagtatccc gacgtcctcc gtcccgatgc
     9421 gtggattctt tgcgacgggc cggttcacca gagccggcgc atgcaactcg tcttcggggc
     9481 gcgcggcgtg gtcgatctcg agctcacggt ctacggtccg gtgaaaggac tccacgacgg
     9541 ccattacggc aactgggtcc cgaacccgat cgtccggctc acgcacctga tcgattcgat
     9601 gcgcgacgag agcggtcgca ttctcatcgc gcatttctac gacgacgtcc ggccgtcgtc
     9661 caaggccgag caggacgcga tcgcgaagat gcctcccgtc gagtcggacc tgaaacacga
     9721 gttcgcaatc gcagagaccg aagggaacgg ccgcccgctg aacgagctac tgatgctgcc
     9781 cgcgttgaac gtccgaggca tccagtcggg acacgtcggc gcgcaggcgt cgaacacgat
     9841 tcagaccgag gcggtggcgt cgctcgactt ccgcctggtg cccgcggaaa cgccggagtc
     9901 ggtaaaggcg ctcgtcgagc accacctcga aaccctcggc tacacgatcg ttcgcacaac
     9961 cccggacctg gcagtgcgcg ccgcgcacgc gaagctcgtc aaagccgttt ggggctccgg
    10021 ctatccaccc gcccgcacgc cgctcgacct ccccttctcg aaccagatcg cgacgctcat
    10081 gaccaccgct ggccacgatc ctgtccgcgt ccccaccctc ggcggcagca tcccgatgta
    10141 cctcttccag cagcccgcca acacgccggt actggtcctc cccatcgcca accacgacga
    10201 caaccagcac gccgcaaacg aaaatatccg tctgcagaat ctgtgggatg ggattgatgt
    10261 gtatgtggcg ctgttcgagg ggttgaagta gcgtttcgac cgccgctacc atttttcctt
    10321 cttgaattac tccgcaagcg aacctacata gttaccggga gcgggctctt cggcaacaag
    10381 ttgatcgtcc gcgtgagcct tgccctacgg aggattcaaa tgatgagaga gcgattcggc
    10441 gtgattcttt tcagtctttt tttcgccagc gtggcggcgg cgcagttatc ccgcaatcca
    10501 tgcataaact tcccttgccc ggcgcttcag tcctgcatct cgccggccga cgcaccgtcc
    10561 tgcgttgccg attccccaac ccgctgctcg gtcgtcacgg tctacacgca cgggctgatg
    10621 gactcgttcg ctcaacccgt tgacccgacg acgccgagcc cgaccctcac cacgttcctc
    10681 ggcgccgcaa tcagcaggta cgacacggcg agctgcaacc agcatttcgg ggacagcttt
    10741 ccggtcggct cttgtctgaa ttgcaagttc tgcggcgcca aggttgagat ccacttgaag
    10801 ccatgcggaa gtgatcagcc cgggaacgac gactacgtga tcggcgttgc acccttcggc
    10861 acaactcaga tcctcgcgtc gggtcgaatc tggccaacag gtgtcacgcc gagggaggag
    10921 attcttgtgg tcaacatccc ggccgacaaa ctcaatcaat tgttttgcaa cgtacgcgtc
    10981 aggccaccca ctctcgacgt ctacgtggaa gacgacacca tcgtcgactg ggtgcgcgtg
    11041 acgatctact cgtcctaagc tgccacgaga acaccgctcc aagttcgtga tcgcgagcca
    11101 tcgaccgcga tggctcgcga ctgacgctct tttgcatcca tcgcgcacga tctaaactga
    11161 cgcgatgtcc aaagcccgcc tcgaaacctt taccgacggc gtggtcgcca ttctcatcac
    11221 gatcatggtg ctcgagctcc atgtaccgca tgggacggcg tggagcgcgc tcgggccgct
    11281 cattccgtcg ctcctcacct acgcgctcag cttcatcttt ctggccatct actggaacaa
    11341 ccatcaccac atgtttcacg ccgtcaaccg cgtgaacggc cggatcctct gggcgaacat
    11401 gcacctgctc ttctggctct cgctggtgcc gttcatcacc ggatggatgg gagagaacaa
    11461 cttcgcgtcg attccgttgg cggcgtacgg agtcgtcctg ctcatggcgg cgatcgcgta
    11521 cacgatcctg cagttcgcaa tcatcgccga ccagggtccg cactcgcccc tcgctgccgc
    11581 ggtcggcgac gacgtcaagg gaaagatctc tgcggcgctg tacgcgatcg cgattccgct
    11641 ctcgttttac caacgctgga tcgcgggcgc gatctacttg actgttgcga tgatctggct
    11701 tgcccccgac cggcgcatcg agaagaaagt tgagtcaggc gttcttccgc acgagaagtg
    11761 ataatcgttt cacgcggagg cgcggaggac acggaggtaa ccaactccgc gcctccgcgt
    11821 ctccgcgtga aacggttctt ggaaactatc cgaacgttcc tgtgaggctt tccgcgactt
    11881 cgccatcgct gtggtagaag tcgcgccaag gagctacaca tgaaaagagt catcttcgca
    11941 gccgccgcaa ccgccttgct ggcgttcaac gcatttgccg ccgacgcgcc tcagccgccc
    12001 gcggaagg
//
LOCUS       DATLVW010000160         2873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_88299, whole genome shotgun sequence.
ACCESSION   DATLVW010000160 DATLVW010000000
VERSION     DATLVW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88299"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..2224
                     /locus_tag="VNN25_05235"
     CDS             275..2224
                     /locus_tag="VNN25_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH90962.1"
                     /translation="MRLFGDDIFISYAHADAGEYALALVARLSKRYAVYVDRVGSKAG
                     EQISDKVLRHLKKASMLIVIVSDDATRETSKVREEVELFALTKRPILVIDAGALDRCS
                     WKESFQGLSIAKESPEAFAKHRPSPGVRRSVEQTRKYWTRTNRLLAIAASIIIVTIGV
                     VAVAWRQVDRASREVQRQQSAAAALRLANLATTAWQRGMTPVDDVLLMVAESLRRGAT
                     PSALQLASVARSLRPRFVSRFGIDFEDKMQLQDGRTLVLNKSESNVVEFIDLSTGGAR
                     LATPDCNKIFLDSMTPTAVAVKCSNHDIPRGLFIDLGTGAITPAAIPAGRGQTIAGLI
                     AGGPLANAVVVSAVVFEFRDRTSGERIGEPVTVGNGSGTPPTIQLLTFSEDGRVLAVA
                     STSNAGGDVWLFEVPTGRLCGHLILAGAAGEIALNHDGSALAVKSGTPNDDLGSGTIE
                     MALSSGGQELTLAWRMMFHSGLDQSLHFVGDAVMFRDSDVVRIVAAGERGEAREVRRI
                     VDGGRLNFATVFPDGDVMTVTDDHATRWKVGAIPAPGGYPAGIDSTGSRVVLWRDRTV
                     IVRDLARGADVFRKYFPLESDDDDPADDSQPPVALDAGGHRLTVGFPKHTDTYDVLTG
                     DNCRTCIPIRRAMHGTSFMPATARS"
     gene            2152..>2873
                     /locus_tag="VNN25_05240"
     CDS             2152..>2873
                     /locus_tag="VNN25_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90963.1"
                     /translation="MPNLYPDPTRDAWDVVYAGNGSLVTEADHMEHKPVRFTGVRITV
                     MDPAGKLIDDFNLPAEARSLSSSFDAHWVAYASGGKVYLRDTVRKKSEPIAGAGAALL
                     SSDAKVLAVVSGGRVAVHDLSHRSEVASFKETGTVSQLAFSDDHQRLGLVCGDQVGYR
                     VLLYDLASKQITYEAPADSKPLIAFRPGERTLTVVGPAGDVASVDDDPLRVAGAICKE
                     TGRSLSPDRWSQLLGREPWVDT"
BASE COUNT          518 a          859 c          941 g          555 t
ORIGIN      
        1 cctcgacccc gctacgctcg ttcctgctgc ccgctccggc ccgtttgcgc aagaaaacaa
       61 agcagctggc gccccggaag ggccagtctc gggccgttgg ttcgcagcga ccgtatttca
      121 gcgctttggt accctacttt agcttggaga gaaaaacgga ggcctgccgt gtatcatcct
      181 ttcgtgatcc gcgtctgcat cgtctcgcat caaacgcatc gcgaccggct cgaaatgctg
      241 cagcaggatt tgccggggca gtagcggagt ttcgatgaga ctcttcggcg acgacatctt
      301 catctcgtac gcgcatgccg acgcgggtga atacgcgctg gcgctcgtgg cccggttgtc
      361 gaagcggtat gcggtgtacg tcgaccgcgt cggcagcaag gcgggagagc agatctcgga
      421 caaggtgctg cggcatttga agaaggcctc gatgctgatc gtgatcgtgt cggatgacgc
      481 gacacgagaa acctcgaaag tacgcgaaga ggtggagctc ttcgccctga caaaacggcc
      541 gattctggtc atcgatgccg gcgcgctaga tcgatgttcg tggaaggaga gcttccaggg
      601 actgtccatc gccaaagagt cgcccgaggc gttcgcgaag catcggccct cgccgggcgt
      661 acggcgaagc gtggaacaga cgcgaaagta ctggacgcgg acgaaccgcc tgctggccat
      721 cgcggcgtcg atcatcatcg tgacaatcgg cgtggtcgcc gtggcgtggc ggcaagtcga
      781 tcgcgcgtcg cgcgaagtac agcggcagca gagcgcggca gcggcgctcc gactggccaa
      841 tctggcgacg accgcgtggc agcggggcat gacgccggtc gatgatgttc tgctgatggt
      901 cgcggagtcg ttgcggcgag gcgcgacgcc gtcggcgttg caactcgcct ccgtcgcgcg
      961 gtcgctccgt ccgcggttcg tgtcccgatt tgggatcgac ttcgaggaca agatgcagct
     1021 acaggacggt cgaacgctcg ttctcaacaa gagcgaatcg aacgtcgttg agttcatcga
     1081 tctctcgacc ggcggcgcaa gactcgcaac tccggactgc aacaagatct tcctcgattc
     1141 aatgacgccc acagcagtgg cggtcaagtg ctccaatcac gacataccac ggggtctttt
     1201 catcgatctc ggtaccggcg ccatcacgcc tgccgcgatt cctgccggga ggggacaaac
     1261 gatagccggt cttatcgccg gcgggcctct ggcgaatgcc gtggttgtca gtgctgtggt
     1321 gttcgagttt cgcgatcgta cgagcggcga gcggatcggt gaacctgtca ccgtcggcaa
     1381 cggttcaggc acgcctccga cgattcagct gcttaccttc agcgaagacg gcagggtgct
     1441 ggcggtcgcc tcaacgtcga atgcgggcgg cgatgtctgg ctcttcgagg ttccgaccgg
     1501 caggttgtgc gggcatctga tccttgccgg cgcggccggc gagatcgcgc tcaatcacga
     1561 cggcagcgcg ctggccgtca agagcggcac ccccaacgac gacctcggca gcggcacgat
     1621 cgagatggct ttgtcgagcg ggggacaaga attgacgctg gcgtggcgaa tgatgtttca
     1681 ctcgggactt gaccaatcgc tccatttcgt cggcgacgcc gtgatgtttc gtgacagcga
     1741 cgtcgttcgg atcgtcgccg ccggcgagcg gggtgaggcg cgcgaagtgc gtcgaatcgt
     1801 cgatggcgga cgacttaatt ttgcgacggt tttccccgac ggtgacgtca tgaccgtgac
     1861 ggacgatcat gctacccgct ggaaggtcgg cgccattcca gcacccggag gttatccggc
     1921 gggcatcgat tcaacgggga gccgggtcgt tttgtggcgc gatcgcacgg tgatcgttcg
     1981 cgacctggcg agaggtgccg atgtgttccg caagtacttt cccctcgaga gcgacgatga
     2041 cgatcccgcc gacgattctc aaccgccggt cgctcttgac gccggcggac accgtttgac
     2101 agtcggtttt ccgaagcaca ctgacacgta cgacgtcctc accggggaca attgccgaac
     2161 ctgtatcccg atccgacgcg cgatgcatgg gacgtcgttt atgccggcaa cggctcgctc
     2221 gtgaccgagg ccgaccatat ggagcacaag cccgtacgtt tcactggcgt gcggatcacc
     2281 gtgatggatc ccgcaggaaa gctgatcgat gacttcaatt tgcccgccga ggcacgttcg
     2341 ttgtcgtcga gcttcgacgc gcattgggtc gcgtatgcgt cgggcggcaa ggtgtatctt
     2401 cgggacactg tcagaaaaaa atcggaaccc attgccggag caggggcggc gttgctctcg
     2461 tccgacgcaa aggtcctcgc cgtcgtttcc ggggggcgtg tagcggttca cgatctcagc
     2521 caccggagcg aagtcgcttc attcaaggag acgggcacgg tcagccagct ggccttcagc
     2581 gacgatcacc agcgtctcgg ccttgtctgc ggcgatcagg tcggctatcg cgtgctgctc
     2641 tatgacttag cgtcgaagca gatcacgtac gaggcgccag ccgacagcaa gccgttgatc
     2701 gcgttccggc cgggcgagcg tacactgacg gtagtcggcc ccgcgggtga tgtcgcttcc
     2761 gtcgacgacg atcctcttcg cgtcgcgggt gcgatctgca aagagacggg ccgatctctg
     2821 tcacctgatc gatggtcgca actgctgggc cgtgagccgt gggtcgacac ctg
//
LOCUS       DATLVW010000161        14494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_253660, whole genome shotgun sequence.
ACCESSION   DATLVW010000161 DATLVW010000000
VERSION     DATLVW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14494
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253660"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VNN25_05245"
     CDS             <1..629
                     /locus_tag="VNN25_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH90964.1"
                     /translation="GLIYICNPNNPTGSVTPRAALRSFLTSVPSTTAILVDEAYFHYA
                     SSADYESVIPLIASRPNLIVARTFSKIYGMAGLRCGYAVSSRELIKQLAAQQAWDSMN
                     IMAIAAAQASLGDRQHVIDGSKRNAATKADVVASLKNLGYEIIPSETNFIMINVRRDV
                     RPLLAAMHTHGVHVGRLFPALPQHMRVTIGKPDQMQAFVKAFGEVVKG"
     gene            778..954
                     /locus_tag="VNN25_05250"
     CDS             778..954
                     /locus_tag="VNN25_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90965.1"
                     /translation="MAIPGRVAERLSAGLKRFGPILSSAKSRDVNESHTSMIVTDLLA
                     DIIGYDKSDQAVGG"
     gene            954..1634
                     /locus_tag="VNN25_05255"
     CDS             954..1634
                     /locus_tag="VNN25_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXH90966.1"
                     /translation="MDTHRAAIRNEILIVLLALASCSLLVFELVSDASQEQMAWIQRI
                     DLAIALVFLGEFLIRLAKADNRRQFFRRFWWELLAAIPITGHAARALRGLPLLRTIRV
                     IRLLRLVRLTARVRVLIKDSHAFIHGAWLIEISTSVVALVSAAALSFHYFEHGTNPNV
                     HTVADSFWWAIVTVTTVGYGDIYPITTGGRLIAVGLMLVGIGTLGLYSAAIASFVVKR
                     HMEEEDRK"
     gene            complement(1914..2429)
                     /gene="def"
                     /locus_tag="VNN25_05260"
     CDS             complement(1914..2429)
                     /gene="def"
                     /locus_tag="VNN25_05260"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXH90967.1"
                     /translation="MSILKVSRLGHPVLRLKSSDVEVADIKAGKFRALIDDMIETMHE
                     YEGVGLAGPQVHLPLRIFVFEVQEKVAKRRGISKVGAGVFFNATYEPIGDDTITDWEG
                     CLSVPFLGGETPRYKRVRLRGLDHEGNPAELEVSDFTARIFQHEIDHTDGHVYLDRMP
                     DLTTLGYTVVL"
     gene            complement(2440..3657)
                     /locus_tag="VNN25_05265"
     CDS             complement(2440..3657)
                     /locus_tag="VNN25_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH90968.1"
                     /translation="MKKTSLAVLFLTVFIDLIGFGMVIPFLSFYAREYGANGTTVGMV
                     VGIYSIMQFFFAPVWGRLSDRIGRRPVILISLTASCIGYFLFAFSRGLTLLFLSRIIA
                     GAGGANIGTAQAYIADKTTPENRAKGMGIIGAAFGMGFIFGPPLSGVLSYIGTSHGMA
                     GNLLPGAVAGSLSLIALMIAFFVLGESKPPDLQPRSGIPPQFDKRVWRGLATHGLLLA
                     IIATIFITLLAVAGMETSVTLHARDRFHFRQPDLSWFFLFMGVIVAGIQGGLIGKLAK
                     RFGEKALIAAGAASFTIGLAFVPLVWRVPLLYVVAFFIAVGQGLTYPSLTSLLTKASP
                     SSEHGTMLGLASGIGSLARFIGPIWCGFLYDLAQARGAFYGSAVLTAIAFVIALRMRK
                     QPLLVDHATSAAG"
     gene            3690..4373
                     /locus_tag="VNN25_05270"
     CDS             3690..4373
                     /locus_tag="VNN25_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HXH90969.1"
                     /translation="MRKLVLFDIDGTLISDQGASREAFATALADVYGFRGDLRRYDFS
                     GRTDPQIVRMILHDDARTDGDIDSQLPLFWQHYLGGLARNAPGRVRMLPGIRALLDAV
                     CADDHLTVALLTGNIEPGARLKLGAIGLNDTFAFGAFGSDSPRREELPPIAATRAADR
                     TGHAFDGGNVVIIGDSIYDVRCGIPHGATTIAIASGKTSADTLRAENPDHFFESAEDV
                     EGIMAAMKG"
     gene            4564..5358
                     /locus_tag="VNN25_05275"
     CDS             4564..5358
                     /locus_tag="VNN25_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXH90970.1"
                     /translation="MSHYLLPLLFIAIIYVAMIVMNERYRLFSSDHFSSNVTKVVAYL
                     WLALLMFGLSTLVVGASLRVPTARDLSRVPFYSLFGLHAILIVFLAGWWLLTKRPPLG
                     EFFSIPRRGNGDAILTGFAVGVGGWAVTLSVALIIALLLMASGLIPKHPAPSPMIGWM
                     AALPLWKKATIVLSAMTVEEAFFRGWLQKRIGVIASTALFALAHSGLGQPLLLIGVAV
                     ISLIIGFTFYRTKNLIPGIIAHGIFDAVQLFVLIPVAFKMSGMGAP"
     gene            5864..7003
                     /locus_tag="VNN25_05280"
     CDS             5864..7003
                     /locus_tag="VNN25_05280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HXH90971.1"
                     /translation="MTSTPARERIVLIDDSTNDLEVTRRFLERRGFDVSAATSGEEGL
                     ALAQAITPAAFIVDYRMPIMDGFEVTRRIKADPLLQTIPVLMLTGADTSQTVVEGLEA
                     GADDFVTKGSDTEVLLARLRALLRVKRYQDQLRKLNQQITRDLQIARRVQEALVPNGT
                     FRGPHIEIRSAYIPSETLSGDFYDYFLQDELMYLFVADVSGHGLPASILVALLKSYIH
                     TEADALKPLSSFMASLNDFLFSVSLPTQFATAQLFRIDAAGNLVYSNAAHPPFLLFKR
                     SSGQTQIHESASHLLGAMPSMVFEEQRLQVAPGDTLFVHTDGLTDRRNESGEFYSIDR
                     IVALLNNSGDADLNTLYDQIYEDVSTFAATEEMRDDIAFVVTRFQ"
     gene            complement(7165..8664)
                     /gene="ahcY"
                     /locus_tag="VNN25_05285"
     CDS             complement(7165..8664)
                     /gene="ahcY"
                     /locus_tag="VNN25_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXH90972.1"
                     /translation="MTATLEVTHSFEASRAAGREPYKVRDLSLAALGRNEMRLAEHEM
                     PGLMAVREKYGKSKPLAGARVMGSLHMTVQTGVLIETLAILGADVRWVSCNIFSTQDH
                     AAAAVVVGRPESGGTTDDPRGVPVFAWKGETLEEYWWCTSEALLWPDGGGPNLIVDDG
                     GDATLLVHKGAEFEGKGHVPEFNPADDPEEWGVILDLLRAEAKGNPKRWTKVAADIKG
                     VSEETTTGVHRLYQMMEAGDLLFPAINVNDSVTKSKFDNIYGCRHSVVDGLNRATDVM
                     IAGKLSVVCGYGEVGKGCAQALRGQGARVVVTEIDPICALQAAMEGFEVRTLESVVER
                     ADIFVTATGNKNVITADHMSRMKNKAIVSNIGHFDNEIDMAGLKKFAGVERVTIKPQY
                     DEWVFPDGHSVLILAEGRLMNLGCATGHPSFVMSASFTNQVLAQLALWQNKGEYQKKV
                     YVLPKKLDEEVARLHLAHLGVELTKLSAEQSKYLGTPPEGPYKADHYRY"
     gene            9126..9374
                     /locus_tag="VNN25_05290"
     CDS             9126..9374
                     /locus_tag="VNN25_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90973.1"
                     /translation="MTRQTIRNLRVPLPEETYDQLKSESNRAHRPATTLAREAISHWL
                     EERRRSRLAGDIETYAREHANTDADLDADLERATLMLA"
     gene            9435..9728
                     /locus_tag="VNN25_05295"
     CDS             9435..9728
                     /locus_tag="VNN25_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXH90974.1"
                     /translation="MTVSVTEFRARCLEILREAERDRKVVEITRRGVVVARLLPAAAD
                     SRVKARPWERLRGSGMLFGPPEQGSRSPFDVEGADLGLKREQIVSAIRDSREK"
     gene            complement(10151..10384)
                     /locus_tag="VNN25_05300"
     CDS             complement(10151..10384)
                     /locus_tag="VNN25_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90975.1"
                     /translation="MNTIFGGQTRWTPVKRDRTAVKRDGTVVKRDGTPVKRDGTLIKR
                     DSTPVKRDNTLVKRDSTPEKRVSTPGQARQYAG"
     gene            10837..11385
                     /locus_tag="VNN25_05305"
     CDS             10837..11385
                     /locus_tag="VNN25_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90976.1"
                     /translation="MSEITQPPKNPATEAGATPALSPDEVVAQLRALRQQIGEVTPLT
                     KQQRIALRAHAALPNDVVQASINALGASDGVQQVLGQPVDEARQMAEDANRWIAVEDE
                     LRAMLKGISGANLIRRQRVGLLSVQAYVISQQLVRDPSNASLLPHVEEIKRLRSLGRR
                     KKPATPAPQEPPVVVTSQHLDS"
     gene            11467..11943
                     /locus_tag="VNN25_05310"
     CDS             11467..11943
                     /locus_tag="VNN25_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90977.1"
                     /translation="MKNPYEEEAARYMAALQAIGSAINDLAATAPPPPGPMSRRARKV
                     TPAVIRRWIADLRSGKMRPIDPLKDPNILADELEGELMIIAAMVHAGSMLQRVKGSLL
                     SRIETKAKKTLAEAMTIFREARGLARTSDDEALKERVRRMDRALGRGVRRKVRIRR"
     gene            complement(12048..13499)
                     /locus_tag="VNN25_05315"
     CDS             complement(12048..13499)
                     /locus_tag="VNN25_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH90978.1"
                     /translation="MPTQRIDMHTHVFNVRYLPVEGIIRSRGVPDLIAKGLAKVLNAR
                     TGDDIEPSAPLFAAAVAADAQGADELPAVHAQTSGEAIDQLASGTPKELIDDPDVRAA
                     LDHPDVRVALAAMQGLHVGELQALAMNHVESADVEPQFAALYGQLSRMEPASGIFETG
                     AEYLRWFNFLTHSERVIMQTLLATYGDDVHLFIHHMMDMEAYYHHGRCYYDFVNDQLP
                     RMRRLVNASGGRLLTFVAYSPDRPNDLNVVRRALDDTDAVGVKFYPPNGYRPDEARHD
                     ALYTELVHRDAPLFAHCTPTGMEATPGSGANSNPTYWANVLGRFPSLRLCLGHAGGDE
                     PWFGLAPWDGSYAAQAVALATRYDNVYLEFGYHDHILDPAVREGFIARLSGLIAANAG
                     LEKKIMYGTDWHMIEKINNHQEYFHDFAAAFADPRLSSYSDAFFYTNAVSYLRLPQFR
                     DSRAAINGVDDPVVGNLDRVIAVAEQMGIDHNP"
     gene            13832..>14494
                     /locus_tag="VNN25_05320"
     CDS             13832..>14494
                     /locus_tag="VNN25_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90979.1"
                     /translation="MPTNRNSTHLRISLLFIVFIGAARLGIAQENPYFITYTHHMEEP
                     GSLEVALNPVLGNPKGGAKHFLASSLELEYGVNGWWTSELYLDGQKTRPESASFTGYR
                     FENRFRLLMKEHAINPVLYVEYEDITGADKILKEVVGFDSFRDLLEPAEDLRHEKKRE
                     VEAKLILSSNRRGWNFAGNLIAEKNLNGDPIEFGYAAGVSRPLVLAATPNPCSLCAEN
                     FTL"
BASE COUNT         2832 a         4552 c         4337 g         2773 t
ORIGIN      
        1 caggtttgat ctacatctgc aatccgaaca atccgaccgg cagcgttacg ccacgcgcgg
       61 ccctgcgtag tttcctgacg agcgtcccgt cgaccacggc gattctggtc gacgaggcgt
      121 actttcacta cgccagcagc gccgactacg agagcgtgat cccgttgatt gcatcgaggc
      181 cgaatctgat cgtggcgcgc acgttctcca agatctacgg catggccgga ttgcgctgcg
      241 gctatgcagt cagctcgcgt gagctgatca aacagctcgc cgcacaacaa gcctgggact
      301 cgatgaacat catggccatc gccgccgccc aggcctccct cggcgaccgg caacacgtca
      361 tcgatggcag caaacgaaat gccgcgacga aggcggacgt cgtggcatcg ctcaagaacc
      421 tcggctacga gatcatcccg tccgaaacga acttcatcat gatcaacgtc cgccgcgatg
      481 tacggccgct gctcgccgcg atgcacacac acggcgtcca cgtcggccgc ctgttccctg
      541 cgctgccgca acacatgcgc gtcacgatcg gcaagccgga tcagatgcag gcgttcgtga
      601 aggcgtttgg ggaagtggtg aaaggctgag cagccgcaag agcgattgaa cgacccccgt
      661 cagatggggg cagcagccga ttcgctcgag tgcaaaagag actcgacgcc acaaaacgcc
      721 agggatcccg tctttccttc agtcatccgc gaaagctgat gcacaaggag aatgacgatg
      781 gcgatccccg gtcgcgtggc agaacgattg tccgccggat taaagcggtt cggcccgatt
      841 ctgtcctcgg ccaagtcgcg agacgtgaat gagtcacaca cgtcaatgat cgtcacggat
      901 ctccttgcgg acatcatcgg ctacgacaag tctgatcagg ccgtcggtgg ctgatggaca
      961 cgcaccgcgc tgcgattcgc aacgagatcc tcatcgttct tctcgcgttg gcgagttgct
     1021 cgctcctcgt cttcgagctc gtctccgatg cttcgcaaga acagatggcg tggatccaac
     1081 gaatcgacct cgccatcgcg ttggtattcc tcggtgagtt cctcatccgg cttgcgaagg
     1141 ccgacaatcg gcggcagttt ttccgtcgtt tctggtggga gctgcttgcg gcgatcccga
     1201 ttactggtca cgcggcaagg gcgttgcggg ggctgcccct gcttcgcacc atacgcgtca
     1261 tccggctgct ccgtctcgta cggctgaccg ccagagtacg cgttctcatc aaagactcgc
     1321 acgcgttcat tcacggagca tggctcatcg aaatctcgac gtccgttgtc gcgctagtct
     1381 ccgcggctgc gctttcgttc cactatttcg aacacggcac gaacccgaat gtgcataccg
     1441 ttgcggacag cttctggtgg gcaatcgtca cagtaacgac tgtcgggtac ggcgatatct
     1501 acccgatcac gaccggcggc cgcttgatcg cggttggact catgctcgtc ggaatcggaa
     1561 cgctgggcct ctacagcgcg gcgatcgcga gtttcgtcgt gaagcggcac atggaagagg
     1621 aagatcggaa gtgagaggac cgagtgcgct gtaaacctct ctttttcctg gagcaatctt
     1681 caatcgagcg ccgaaggacg gatgaggggg gtgtagcaat cgacgggctc gaagttctcg
     1741 gaaacgtgaa cgccatcgct tcggcaacgt tgtcgcaaca gtcaaacgcc ccctcatccg
     1801 gcttcgccac cttctccccc acaagaagcg tgggggagaa ggcgcgcgat tggaaaagta
     1861 aggcgggatg ggtgagtaag aaatgcgact gcagagctaa ttcggtttgt ttcttacaac
     1921 accaccgtat atcccagcgt cgtcagatcc ggcatccggt cgaggtagac gtggccgtcg
     1981 gtgtggtcga tctcgtgttg aaagatgcgg gcggtgaagt cggacacttc cagctcggct
     2041 ggattgcctt cgtggtcgag gccgcggaga cggacgcgtt tgtagcgcgg agtctcgccg
     2101 ccgaggaagg ggacgcttag gcagccttcc cagtcggtga tcgtatcgtc gccgatcggc
     2161 tcgtacgtcg cgttgaagaa gacgccggcg ccgaccttcg agatgccgcg gcgtttggca
     2221 actttctcct gcacctcgaa cacgaagatg cgcagcggaa ggtgaacttg cggaccggcc
     2281 agtccgacgc cttcgtattc gtgcatcgtc tcgatcatgt cgtcgatcag cgcgcggaac
     2341 ttgcccgcct tgatgtcggc gacttcgacg tcggaggatt tgagccgcaa gacggggtgc
     2401 ccgaggcggc tgactttgag gatcgacatg aggcgaagtt taacctgccg cgctggttgc
     2461 atgatcgacg agcaacggct gctttcgcat tcgcagcgcg atgacgaacg cgatcgccgt
     2521 caagaccgcg ctgccgtaaa acgcgccgcg cgcctgggcc agatcgtaga ggaatccgca
     2581 ccagatcgga ccgatgaatc gcgccagcga gccgatgccg gaggcgagtc cgagcatggt
     2641 tccatgctcg ctcgacggcg atgctttcgt cagcaacgac gtgagcgacg gataggtcag
     2701 cccctggccg acggcgatga aaaacgcgac cacgtacaac agcggcacgc gccagacgag
     2761 gggaacgaag gccagtccga tcgtgaacga tgcggcgccg gcggcaatga gcgccttctc
     2821 gccgaagcgc ttggccagct tcccgatcaa gcctccctga atgccggcca cgatcacgcc
     2881 catgaaaaga aagaaccacg acagatcggg ctgcctgaaa tggaatcgat cgcgcgcgtg
     2941 cagcgtgacg ctggtctcca tgcccgccac cgcgagcagc gtgatgaaga tcgtcgcgat
     3001 gatcgccagc agcaacccgt gcgtggccag cccacgccaa acacgcttgt cgaactgcgg
     3061 cgggattccg ctgcgtggct gcaagtccgg cggtttcgac tcgccgagta cgaagaaggc
     3121 aatcatcagc gcaatgagcg aaagcgagcc ggcaacagct cccggaagga gattacctgc
     3181 catgccgtgg ctcgtcccga tgtacgacaa cactccgctg agcggtggtc cgaagatgaa
     3241 gcccatcccg aacgcagcgc cgatgatccc catccctttc gcgcgattct cgggcgtggt
     3301 cttgtcggcg atgtaggcct gtgctgtgcc gatgttcgcg ccgcccgcgc cggcgatgat
     3361 gcgcgagagg aagagcagtg tcaggccgcg cgagaacgca aaaaggaagt agccgatgca
     3421 gctcgcggtg aggctgatga gaatcaccgg acgtcgcccg atgcggtccg agaggcgccc
     3481 ccacaccggc gcgaaaaaga actgcatgat cgagtagatg ccgaccacca tccccaccgt
     3541 cgtcccgttt gcgccgtatt cgcgggcgta gaacgagagg aacgggatca ccatcccgaa
     3601 tccgatgagg tcgatgaaaa ccgtgaggaa aaggacggcc agagacgttt tcttcataga
     3661 aggcgcgcga agtgtactct gtcactccga tgcgcaaact cgttctattc gacatcgacg
     3721 gcacactcat cagtgaccaa ggcgcttccc gcgaagcctt cgcgacggca ctggcggacg
     3781 tctacggatt tcgcggcgat ctgcgccgct acgacttctc cggccgcacc gatccgcaga
     3841 tcgtgcgcat gattctgcac gacgacgcac gcaccgatgg tgacatcgat tcccagctcc
     3901 ctctcttctg gcagcactac ctcggcggcc tcgcgcgcaa cgcgcctggc cgcgtccgca
     3961 tgctgcccgg catccgcgcg ctgctcgacg cggtttgcgc tgacgaccat ctgactgtgg
     4021 cacttctcac cggcaacatc gagccgggcg cgcgtctcaa gctcggtgcc atcggattga
     4081 acgacacttt cgcgttcggc gccttcggca gcgactcgcc gcgccgcgaa gagttaccgc
     4141 cgatcgccgc cacgcgtgca gccgacagga ccggccatgc cttcgacgga gggaacgtcg
     4201 tcatcatcgg cgactccatc tacgacgtcc gttgcggcat cccccacggc gccacgacga
     4261 tcgcgatcgc gtccgggaag acgtccgcgg atacattgcg cgcagaaaat ccggatcact
     4321 tcttcgaatc agccgaggac gtggagggga tcatggccgc gatgaaggga tagcgttttg
     4381 cgctacgcgc gttgtcgggt gtcgggtgat tgcgcttcgc gcgttgtcag ccgagacgta
     4441 ttgataccgt tgcgggatac tcaagcactg tatcgatctc gactttcttc tgacaacgcg
     4501 cgaagcgcat ctaaccgaca accgacaacg cgcgcagcgc atcgattaca ctttgcgcgc
     4561 cagatgagcc attacctgct gccccttctc ttcatcgcca tcatttacgt cgcgatgatc
     4621 gtgatgaacg agcgctatcg tctcttttcg agcgatcact tttcgtcgaa cgtgaccaaa
     4681 gtcgtcgcct acttatggct ggcgctgctc atgttcgggc tgagcacgct ggtcgtcggc
     4741 gcatcgctgc gggtaccgac ggcgcgagac ttgtcgcgcg ttccgtttta ctcgctcttc
     4801 ggcctgcacg cgattctgat cgtcttcctc gccggctggt ggctcctcac gaagcgtccg
     4861 ccgctcggcg agtttttcag cattccgcgg cgcggcaacg gcgatgcgat cctgaccgga
     4921 tttgcggtcg gcgtcggtgg ctgggccgtc acgctctccg tcgctctgat catcgcgttg
     4981 cttctcatgg cgtcgggact catcccgaaa catccggcac cgtcgccgat gatcggctgg
     5041 atggcggcgt tgccattgtg gaaaaaggcg accatcgtcc tttccgcgat gacggtcgag
     5101 gaggcattct tccgcgggtg gctgcagaag aggatcggcg tcatcgcctc gactgccctt
     5161 ttcgccctcg cccactccgg cctcgggcag cccctgctgc tgatcggcgt ggccgtgatc
     5221 tcgctgatca tcggcttcac gttttaccgg acgaagaacc tgatcccagg catcatcgcg
     5281 cacggcatct tcgacgcggt gcagctcttc gtgctcatcc cggtggcgtt caagatgtcg
     5341 ggaatgggcg cgccgtagat tccgaatcgg agccggacgc ggtcatcggc cggcagcgtg
     5401 cgacgaacgc gggaatcggc gctgcaacga cgcgcctcgt gcagactctc accttcggcg
     5461 cggtattggc gggcgtcgcg gacgcgcgca ctggcgtcga ctgcagagcc ggacgcgtgc
     5521 tgctttgcgt cagcacggtc cggccctgca gatcgatcgc ggactcgaga acgaacgttg
     5581 taatcgcccg gatcgcagcg cgtttcggat tctcgggaag gtgacaggcc agtaccagag
     5641 aaggtagaag gaggagcgcc gccgttcgtc tcacataacc taagacgacc gatgcgcccg
     5701 aaagttgggc caaaaccagc gacgtgacga ggttgtaaca cgacctccat cgctgccaca
     5761 gcaaggcgac caccgagata cagaggacac cgagaaacct ctgtgtcctc tgtgcctcgg
     5821 tggtgaaagg ttttctcccc ctcccggaat gtagaatccc gccgtgacct ccacccctgc
     5881 gcgtgagcgg atcgttctga tcgacgacag cacgaacgat ctggaggtga cgcgccgctt
     5941 tctggaacgc cgcggtttcg acgtctcggc ggcgacatcc ggcgaagagg gactggcgct
     6001 tgcccaggcc atcacccctg ccgccttcat cgtcgactat cgcatgccga tcatggacgg
     6061 cttcgaagtc acgcgccgta tcaaggccga tccgctgctg cagacgattc cggtgctgat
     6121 gctgacgggt gccgatacgt cgcagacggt cgtggaaggg ctcgaagccg gtgccgatga
     6181 tttcgtcacc aaaggctccg ataccgaggt gctgctggcg cgcctccggg cgttgttgcg
     6241 cgtcaagcgt taccaggacc agctccgcaa attgaatcag cagatcacac gcgacctgca
     6301 gatcgcccgc cgcgttcagg aagcgctcgt tccgaacggc acgttccgcg gaccgcacat
     6361 cgagatccgc tccgcctaca ttcccagcga aacgctctcc ggcgatttct acgactactt
     6421 cctgcaggac gagttgatgt acctcttcgt cgcggacgtg tcagggcacg gacttccggc
     6481 ctcgatcctc gtcgcattgc tcaagagcta tatccacacc gaggctgacg cgctcaagcc
     6541 gctgtcgtcg ttcatggcca gtctcaacga cttcctcttc agcgtttcgc ttccgacgca
     6601 gttcgccacc gcgcagctct ttcgcatcga cgccgccggc aatctcgtct attcgaacgc
     6661 cgcgcatccg ccgtttctgc tgttcaagcg cagcagcggc cagacccaga tccacgagtc
     6721 ggccagccac ctcctcggcg cgatgccctc gatggtcttc gaagagcaac gcctgcaggt
     6781 cgcgcccggc gacacgctct tcgtccacac cgacggtctg accgatcgcc gcaacgagtc
     6841 gggcgagttc tactccatcg atcgcatcgt cgcgctgttg aacaactcag gcgacgcgga
     6901 cctcaacacg ctctacgatc aaatctacga ggacgtctcc acgttcgccg cgaccgagga
     6961 gatgcgcgac gacatcgcct tcgtggtcac ccgctttcaa tgagcaaaaa gaaactcgga
     7021 gacgcggagt ggtcctgaga tcagagacaa gagatcagag actagagatg gagatcacgt
     7081 ctctgatctc tggtctctga tctctgtctc ttgtctctgc tcttctcccc gcgtctccgc
     7141 gtgaaatgct tttcgaaacg aacctcaata ccgatagtga tccgccttat acggcccttc
     7201 cggaggcgtc ccaagatact ttgactgctc ggccgacagc ttcgtcagct cgacgccgag
     7261 gtgcgcgaga tgcagccgcg ccacttcttc gtcgagcttc ttcggcagca cgtacacctt
     7321 cttctgatac tcgcccttgt tctgccagag cgcgagctgc gccagcacct ggttcgtgaa
     7381 tgacgccgac atcacgaacg acggatgccc tgtcgcgcac ccgaggttca tcagtcgccc
     7441 ctcagcgagg atcagcacgc tgtggccgtc cgggaagacc cactcgtcgt actgcggctt
     7501 gatcgtcacc cgctcgacgc cggcgaactt cttcagcccc gccatgtcga tctcgttgtc
     7561 gaagtggccg atgttgctga cgatggcctt gttcttcatc cgcgacatgt gatcggcggt
     7621 gatgacgttc ttgttgccgg tggcggtgac gaagatgtcg gcgcgctcga cgactgactc
     7681 cagcgtccgc acttcgaacc cttccatggc cgcctgcagc gcgcagatcg ggtcgatctc
     7741 ggtgacgacg acgcgtgcac cctggccgcg caacgcctgt gcgcaacctt tgcccacttc
     7801 gccgtaaccg cagacgaccg acagcttgcc ggcgatcatg acgtcggtgg cgcgattgag
     7861 gccgtcgacg acggagtggc ggcatccata gatgttgtcg aacttcgact tggtgaccga
     7921 gtcgttgacg ttgatggccg ggaagagcag atcgcccgcc tccatcatct gatagagcct
     7981 gtgtacgccc gtcgtcgtct cctcggacac acccttgatg tcggcagcga ctttcgtcca
     8041 gcgcttcgga ttgcccttcg cctcggcacg aagaagatcg aggatgacgc cccactcctc
     8101 cggatcgtcc gcaggattga actccggaac gtgtcccttc ccttcgaact cagcgccctt
     8161 gtgcacaaga agcgtggcat cgccgccatc atcgacgatg aggttcggcc cgccgccatc
     8221 cggccacaac agcgcctcgg aggtacacca ccaatactcc tcgagcgtct cgcccttcca
     8281 ggcgaagacg ggcacgccgc gcggatcgtc cgtcgtcccg cccgattccg gccgcccgac
     8341 gacaaccgcc gccgccgcat gatcctgcgt cgaaaagatg ttgcagctga cccagcgcac
     8401 atcggcgccg aggatggcca gagtctcgat gagcacgccg gtctgcacgg tcatgtgcaa
     8461 cgaccccatg acccgcgccc ccgccagcgg cttcgacttc ccatacttct cccgaaccgc
     8521 catcagtcca ggcatctcat gctccgccag cctcatctcg ttgcgcccaa gcgcggccag
     8581 cgacagatcc cgaaccttgt acggctcccg ccccgccgcg cgggacgcct caaacgaatg
     8641 cgtaacttcc agtgttgcag tcatgcgaat gtgttgctcc ttcgaacatt acgaggccgg
     8701 caggaaggcc gtccatcggt gggatgagga ttgtaacgca ggggagggcg ggaaccgagc
     8761 ggacggagga ttactctctc ccgaacgcct cgttcatagc acgagcaatc ttccgcgcgg
     8821 aatgagcgtc gagagaaccg gctagctgca tccacggctg agaatccggc ccggacgaga
     8881 ggtccctgat ctcgacagcc agtttggcat gatcgagcac accgatgaga ggctctccaa
     8941 gtttgagcac gttctcatca gcgaggtact ctgcctcaat cgttctggac atctgcaagg
     9001 ataacagtga cggccgaccc tttcgatgaa aggcaccgaa gtcgcgtttt cggaggcgga
     9061 gtccggtgca ttcacgttgc gtcgcatcgt tcttgaaatg acacaacgaa atacaggagc
     9121 gattcatgac acgtcaaacc atccgcaacc ttcgagtgcc attgcccgaa gagacgtacg
     9181 accagttgaa aagtgagtcg aacagggccc atcggccggc aacgacgctg gcacgggaag
     9241 cgatcagcca ttggctcgaa gaacgtcgcc ggagccggct cgcgggggac attgagacgt
     9301 acgccagaga gcatgcgaac acggacgcgg atcttgatgc cgatctcgag agggctacgc
     9361 tgatgctcgc gtaaccatgg caaagatcgt tagctctcga tgttggcgtc cgttgccgcg
     9421 atatcattct tgaaatgacc gtttcagtaa ccgagtttag ggcgcgatgc cttgagatcc
     9481 ttcgggaggc cgagcgcgat cgaaaggtcg tcgagatcac gcggcgagga gttgtggttg
     9541 cacgactcct tcccgctgcc gccgattcgc gagtcaaagc caggccgtgg gaacgactcc
     9601 gcggctccgg aatgcttttc ggaccacccg agcaagggtc ccgctccccc ttcgacgtcg
     9661 aaggcgccga cctcggtctg aaacgcgaac agatcgtcag cgcgatccgt gacagtcggg
     9721 aaaagtgagt ggctgctcgc tcgtacaccg tcccagtccc gggcataacc tcccgatcca
     9781 accaccgagg cgggacgcct cgatggccgc gggcgaagac gcccgcactc ctttgctgtt
     9841 gcgcggatcc ggcatccaga accggtcgca gtggatttga cgaccttgga cagcccgcga
     9901 cgcaagctat ccctgtcgac ggaaccggca ggcggacaag cggggtacac ggactaccag
     9961 gaccacccgc tgtgccgtcg cgctcatccg ccgtgctgac gcgttttttc gacgaaaatc
    10021 gtgttcatcc gtcgtgttga cgcgttcatc agccgtgctg tcgtgttcat ccggcgtgcg
    10081 gtcgtgttca tccggcgtgc tgtcgcgttt gaccggcgtg ctgtcgcgtt tatccgccgt
    10141 gctgtcgcgt ttatccggcg tactgtcgcg cttgaccggg ggtgctgacg cgtttttcag
    10201 gcgtgctgtc gcgttttacc agcgtgttgt cgcgcttgac cggcgtgctg tcgcgtttga
    10261 tgagcgtgcc gtcgcgcttg accggcgtgc catcgcgttt gaccactgtc ccatcgcgtt
    10321 tgaccgctgt tctgtcgcgt ttgaccggcg tccatcgcgt ttgaccgccg aaaatcgtgt
    10381 tcatcccccg tgttgtcccg tttttctgcg gaaaatcgcg ttcatccacc gaaaactcgc
    10441 cattgttccg ccaagaatca cgcgtatcgg cattttttac ctagacattt aacagatttg
    10501 taacatttgt accccctttt tccgcagacc acctgcgtct aaggtacaga agcgttggtg
    10561 atgtccgcct tcgcttcaca gttaggggct tttggtcgca gtggaccggc cccggcggcc
    10621 aatggacgcg atagtgcttg agtgccggcc atcgcgtccc tgcgggagtc gagctgcgct
    10681 cctcctgccg tgaggcgccc gaggtccggc cggactcggg atacgacaac ccggaaacgg
    10741 cggcgcgtag ccgtccgctc cagccggaag gccgcctgct atgtgcacga ccttccagcc
    10801 atacaccgcc ccgaaagggc agaaggaacc atcatcatgt ctgagattac acaacccccg
    10861 aagaaccccg ccacggaggc gggagcaaca ccggcgcttt cgccggacga agtggttgca
    10921 cagttgcgcg ccttacggca acagatcggc gaagtcaccc cactgacgaa gcagcaacgg
    10981 atcgccctgc gcgctcacgc ggcacttccg aacgatgtgg tgcaggcgtc gatcaacgcc
    11041 ctcggcgcct ccgacggcgt gcagcaggtc ctcggacaac ccgtcgacga ggcgcggcaa
    11101 atggccgagg acgcgaaccg ctggatcgcg gtcgaggatg agctgcgcgc gatgctcaaa
    11161 ggcatctccg gagcgaacct catccgccgc cagcgcgtcg gtcttctttc cgtgcaggcc
    11221 tatgtcatca gccagcagtt ggtgcgcgat ccaagcaacg ccagcctgct tccgcacgtg
    11281 gaggagatca agcgtctgag gagcctcggc cgccggaaaa aaccggccac gccggcgccc
    11341 caagagccgc ctgtcgtcgt aacgtcgcaa cacctcgatt cgtagtagtg catagaggtg
    11401 caccgggcgc gcagtacgcg cgcccggtgc ttttctttgc tttcgtcgac gactatggag
    11461 aacgtcatga agaacccgta cgaagaagag gcggcccgct acatggcggc gctgcaggcc
    11521 atcggatccg cgatcaacga tctcgccgcg actgcgcccc cgccaccggg gccaatgagc
    11581 cgccgcgcgc gcaaagtgac cccggcagtc atccgccgct ggatcgcaga cctgcgctcc
    11641 ggaaagatgc gcccaatcga tccgttaaaa gacccaaaca tccttgcgga cgagctcgag
    11701 ggagagctga tgatcatcgc ggcgatggtg cacgccggct cgatgctcca gcgggtgaaa
    11761 ggttcactct tgtcgaggat cgagaccaag gcaaagaaaa cgctcgccga agcgatgacc
    11821 attttccgcg aagcccgcgg gctggctcgc acctcggacg atgaagcatt gaaggaacgc
    11881 gtgcgccgca tggaccgcgc gttaggacga ggggtgagac ggaaggtacg tatccgcaga
    11941 tgacgcagat ggaggcagat gaggagggat catctgtctg ggctccatcc tcacgaatgt
    12001 ggaacagcaa cagaatcgcc ctgaacgctt cgtggtgttc ggcttggcta cggattgtgg
    12061 tcgattccca tctgctcggc caccgcgatc acgcgatcaa ggttaccgac gacgggatca
    12121 tcgactccgt tgatggcagc gcgcgaatcg cggaactgcg gcagccgcaa gtaggagacc
    12181 gcattcgtgt aaaagaaagc gtccgaatat gacgagagac gcggatccgc gaaagcggca
    12241 gcgaaatcgt gaaaatactc ctggtggtta ttgatcttct cgatcatgtg ccagtcggtc
    12301 ccgtacatga tcttcttctc gagtcccgcg ttcgcagcga taagcccgga aagacgagcg
    12361 atgaatccct cgcgcacggc gggatcaagg atgtgatcgt gatagccgaa ttcgagatag
    12421 acattgtcgt agcgcgtcgc aagtgcgact gcctgggcag cgtaggagcc atcccacggc
    12481 gcgagcccga accacggctc atcgccgccg gcgtgaccga ggcagaggcg caggctcggg
    12541 aacctgccta gcacattcgc ccagtaagtc gggttggagt tagcgccgct accgggagtg
    12601 gcctccatcc cggtgggcgt gcagtgtgcg aaaagcggtg cgtcgcggtg gacgagctcc
    12661 gtgtagagag catcgtggcg cgcctcgtcg ggccggtacc cattcggagg atagaacttc
    12721 acgcccacgg catccgtgtc gtcaagtgcg cggcgcacga cgttcagatc gttcggacga
    12781 tccggactgt acgcgacgaa ggtcagcaga cggccaccag acgcgttgac aagccggcgc
    12841 attcgaggca attggtcatt cacgaagtcg tagtagcagc gtccatgatg gtagtaagcc
    12901 tccatgtcca tcatgtggtg aatgaacagg tgaacgtcat cgccgtaggt agcgagcagc
    12961 gtctgcatga tgacccgctc gctgtgtgtc agaaaattga accagcgcaa gtactcggcg
    13021 ccggtctcga agattccgga cgccggctcc atgcgcgaga gttgcccgta gagagccgca
    13081 aattgcggct caacatcggc gctctcgaca tgattcatgg ccaatgcttg caactcgccc
    13141 acatgaagac cttgcatcgc cgcaagagcg acgcgaacgt cgggatgatc gagcgccgcg
    13201 cgaacatcgg gatcgtcgat cagctccttc ggcgttccgg aagccagttg atcgatcgcc
    13261 tccccggagg tttgcgcgtg gaccgcaggc aactcatccg caccctgagc atcggcagct
    13321 accgcagcgg cgaaaagcgg tgccgagggc tcgatgtcgt cgccggtccg cgcgttgaga
    13381 actttcgcca atccctttgc aatcaggtcc gggacaccac gcgagcggat gatcccctcc
    13441 accggaaggt accgaacgtt gaagacgtgt gtgtgcatgt cgatgcgctg ggtgggcatg
    13501 gcgtgctcct gaatcgggta gattgggcta ctctgctagt gtgacggacg gttgacgcgc
    13561 tttccctttc cgacggtccc ggaccagcgg actcacgaac cgaacatggg gtcagtgcac
    13621 gacaagctca gtctcatgca gcaacttggg gcgattccgc cgtaggacct tcgcttcaca
    13681 gaaccacaga gacgcagagc acacgaataa cctctgtgcc ctctgcgact ctggttcaag
    13741 acctcggcac gattgacgcg atctcccgcg gtgtgtagac tgtgtatgca actgagactc
    13801 agtctcaagc tacgcctcga aggaagaccg cttgcccacc aacaggaact ccacacatct
    13861 gagaatctcc ctgctcttta tcgttttcat cggagccgcg cgcctcggca tcgctcagga
    13921 gaacccctac ttcatcacct acacgcatca catggaggag cccgggagtc tggaggtggc
    13981 gctcaatccg gttctcggca atcccaaagg cggcgcgaag cacttcctcg cctcttcgct
    14041 cgagttggag tatggggtga acggttggtg gacgtctgag ttgtatctgg acgggcagaa
    14101 gacgcggcct gagagcgcct cgttcaccgg ctatcgattc gagaaccgct ttcgtctcct
    14161 gatgaaggag cacgcaatca atcctgtgct gtatgtcgag tacgaggaca tcaccggcgc
    14221 ggacaagatc ctcaaagagg tcgtcggctt cgattcgttt cgcgatctgt tggagccggc
    14281 cgaggatttg cgtcatgaga agaaacgcga ggtggaggcg aagctgattc tttccagcaa
    14341 ccggcgcgga tggaactttg ccgggaatct catcgcggag aagaatttga atggcgatcc
    14401 gatcgaattc ggatatgccg ccggcgtcag ccgtccattg gtgctggccg caactcccaa
    14461 tccctgttct ctctgcgccg agaacttcac gctc
//
LOCUS       DATLVW010000162         3756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_502336, whole genome shotgun sequence.
ACCESSION   DATLVW010000162 DATLVW010000000
VERSION     DATLVW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502336"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VNN25_05325"
     CDS             <1..554
                     /locus_tag="VNN25_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90980.1"
                     /translation="HRVRAWAGYRLPLPAGSLEATVLQSYESGMPYSIAGAINLTRYP
                     GAPANFGYASVPNGLYYFSGRGELRTEATRSTDLALRYSVRVSTVEFFAQGDLLNSFN
                     NAAIFDPQRLSTTVTTAATSATFLPFNPAKTTPVQCPTGASAAACTAMGANYQMASNF
                     GTPLNNLAYQTPRTVRMSLGVRF"
     gene            639..1415
                     /locus_tag="VNN25_05330"
     CDS             639..1415
                     /locus_tag="VNN25_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH90981.1"
                     /translation="MKRVKLLRMRLHVALLLLVAASAFAEEPGHWAEVNGHRMYYETS
                     GSGRPLLLLHGGGNTIHGSFAKQLDVFAKTHSVIAPEQIGHGHTPDVKGPFTYARMAS
                     DTAALLVKLKLKDVDVVGWSDGGIVALILAVQHPELVRRVVVSGANFSPEGYLADDLR
                     QMRARDVESPATRDARLNHLWATSPTKDELSPALLTGLHRRVLVMVGDHDAIQLDHTI
                     ALYRALPDARLCILPGTGHGTFIQRPEWVNAIVASFLDEP"
     gene            complement(1425..1757)
                     /locus_tag="VNN25_05335"
                     /pseudo
     CDS             complement(1425..1757)
                     /locus_tag="VNN25_05335"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(1770..2189)
                     /locus_tag="VNN25_05340"
     CDS             complement(1770..2189)
                     /locus_tag="VNN25_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90982.1"
                     /translation="MSRRFNWQLWSGFALSIAAFVSYFVFFARFPITRDMPWVSLILF
                     AIAIALLVIGLRRATGRRIGAWIVTTLGILVFALFCFDIFIGAKWLPTSSHAPAIGSK
                     APDFVLLDIHRKPVALAGLLTEPATKGVLLIFYRGYW"
     gene            2280..3254
                     /locus_tag="VNN25_05345"
     CDS             2280..3254
                     /locus_tag="VNN25_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXH90983.1"
                     /translation="MLTALLFAAATAALPSLPPMSGTGSLGTPANPTQFTFYVGGDNR
                     PDSGDAPSPGFQSLIPAMQNAMKTTPAAFVLWGGDIIKGKKSSDAKTQYPLVLPLFAK
                     LGVPVFNVPGNHELDKKVSGDCKEGPDDNGKLLADYTASVSQQPNGWFQYGNSVFIGI
                     NTEEVPGKDSQPSGCFNGYVSTTQLSLIQAKIAELPKGTNVFLFMHRPTQDNNSHAMR
                     PDPADSGTKYGKKIMAFVNYVNALTNPNVVYVFASHDHRFYQAVAGTSSKTGFVVTGG
                     GGAPLSGCPDKGRPGAYYHWLQVAVNGATVTVTPVPLTNTTPCGPPPQ"
BASE COUNT          716 a         1189 c         1152 g          699 t
ORIGIN      
        1 gtcatcgcgt ccgcgcctgg gccggttatc gtctgccgct gccggctggt tcgctcgaag
       61 cgacagtcct tcagagctac gaatcaggga tgccgtactc gatcgccggc gcaatcaacc
      121 tgactcgcta ccccggcgca cccgcgaact tcggctacgc atcagtgccc aacggtctgt
      181 actacttcag cggccgcggc gagcttcgca cggaggcaac gcgctcgacg gatctcgcgc
      241 tgcgctacag cgtgcgcgtc tcgacggtcg agtttttcgc gcagggcgat ctgctcaatt
      301 ctttcaacaa tgccgcgatc ttcgatccgc agcgtctcag cacgacggtc acgaccgctg
      361 cgacatccgc gacgttcctg ccgttcaatc cggcgaagac gacaccggtg cagtgtccga
      421 cgggcgcatc ggccgcggca tgcacggcga tgggcgcgaa ctatcagatg gcgtcaaact
      481 tcggcacgcc gctgaacaac cttgcgtacc agacgccacg aaccgtgcgg atgtctttgg
      541 gggttcggtt ctgaacttct gaagcacaga caagagtgtc tgtgccacat aacctccgct
      601 ttgaagcaga cgctgggtac ggccactctt gtctgtgcat gaaacgtgtg aaactgctgc
      661 gcatgagatt gcacgtagct ttgctgttgc ttgttgctgc ctccgccttt gccgaagaac
      721 cgggtcattg ggccgaggtg aatgggcatc gcatgtacta cgagacgagc ggctcggggc
      781 ggccgctgct attgctgcat ggcggcggca acacgatcca cggctcgttc gcgaagcagc
      841 tcgatgtctt tgcgaagaca cattcggtca tcgcgccgga gcagatcggc catgggcata
      901 cgcctgatgt caaaggtccg ttcacgtatg cacgcatggc cagcgacacg gctgcgctac
      961 tcgtcaaact caagctcaag gatgtcgacg tcgtcggctg gagcgacggc gggatcgtgg
     1021 cgctcatcct tgcggtgcaa catccggagc tcgtgcggcg cgtcgtggtc agtggcgcga
     1081 acttttcgcc cgaggggtat ctggcggacg acctgcggca gatgcgcgcc agggatgtgg
     1141 agtcgccggc gacacgcgat gcgaggctca atcacctgtg ggcgacatcg ccaacgaaag
     1201 acgagttgag tccggcgctt ctgacgggac tccacaggag agtgctcgtc atggttggcg
     1261 accacgacgc catccagctc gatcacacga tcgcgctcta tcgcgcgttg cccgacgccc
     1321 ggctctgcat tctgccgggg acgggacacg gaacgttcat ccagcggccg gagtgggtga
     1381 atgcgatcgt ggcgtcgttt ctggacgaac cgtaagtttg tgtctcactg aacgacgcgc
     1441 gccgcctcca gcatctgctc cggccgcgcg cgaatgcgga gatcctcggt caggttgcgc
     1501 cagcgcacga tgccattgcg gtcgaccagg aactcggcgg ggcgtgcgat gtcgtgtccg
     1561 cccgggctct cgcccttgtg aacgaggtcg tatcgccgga tggtctcggt gttgggatcg
     1621 gagagaagcg gaaaggtgta gccggttttc ttcacgagat cgcgcgactc ctcgggagtg
     1681 tcgacgctga tcgcaacggg ttggatgccg gcagcgtgga actcggcgag atggtcttca
     1741 ataccccgta actcggagtt gcagaacggt caccagtagc cgcgatagaa gatgaggagg
     1801 acgcccttcg tcgccggctc ggtcaggagt cctgcaagcg caaccggctt tcgatggatg
     1861 tcgagaagga cgaaatcggg cgccttgctg ccgattgccg gcgcgtgcga ggaggtcgga
     1921 agccacttcg cgccgatgaa gatgtcgaag cagaagagcg cgaagacgag gatgccgaga
     1981 gtggtgacga tccacgcgcc gatcctgcgt cccgtcgcgc ggcgcagtcc gatcacgagg
     2041 agcgcgatgg cgatggcgaa gagaatcagc gatacccacg gcatgtcgcg cgtgatcgga
     2101 aagcgggcaa agaaaacgaa gtagctgacg aacgccgcga tgctgagcgc gaagccggac
     2161 cagagctgcc agttgaagcg tcgggacatg agcccatcat aggcgcagga tccgttggcg
     2221 ccacccaatc aagctggaaa gcagggtagg gatctgcctc tttcgcgact ctccccagca
     2281 tgcttacagc gctcttgttc gccgccgcca ccgccgcact cccgtcactt ccaccaatgt
     2341 ccggcaccgg gtccctcggc actccggcca atccgactca gttcaccttc tacgtcggcg
     2401 gcgacaaccg ccccgactcc ggcgacgctc cgtcgcccgg gtttcaatcg ttgatcccgg
     2461 cgatgcagaa cgccatgaag acgacgcccg ccgcattcgt gctctgggga ggcgacatca
     2521 tcaagggcaa gaaatcgtcc gacgcgaaaa cgcagtatcc gctcgtgctg ccgttgttcg
     2581 ccaagctcgg cgtaccggtg ttcaacgttc cggggaacca tgagctggac aaaaaggtaa
     2641 gcggcgactg caaagaaggt cccgacgaca acggcaaact gctcgccgac tacacggctt
     2701 ccgtttccca gcagccgaac ggctggttcc agtacgggaa ctctgttttc atcggcatca
     2761 acaccgagga ggtcccgggg aaggattctc agcccagcgg ctgcttcaac gggtatgtca
     2821 gcacgacgca actctcgctc atacaagcga agatcgccga actgccaaag gggacgaatg
     2881 tcttcctctt catgcaccgt ccgacgcagg acaacaactc gcacgccatg cgcccggacc
     2941 cggcggacag cggcacgaag tacggcaaga agatcatggc cttcgtcaac tatgtgaacg
     3001 cgctcaccaa tccgaacgtg gtctacgtct ttgcctcgca cgatcaccgt ttctaccagg
     3061 ccgttgcagg cacctccagc aagacaggat tcgtcgtcac gggcggcggg ggcgcgccgc
     3121 tttcgggctg ccccgacaag ggccgtccgg gcgcgtacta tcactggctg caagtcgccg
     3181 tgaatggtgc aacggtcacg gtgactccgg ttccgctcac gaacaccacg ccgtgcgggc
     3241 ctccgccgca atgacgaggt cgattgtcgg gtcatcccaa cggtcttgac gtaaagcgac
     3301 ggaacgccgg cgtcctccgc tttcacctac gttgtccgcg gctagacgcc agcgttcccc
     3361 tgccagagcc gaagagtgct taagtcaagg cttggacacg atccgtgacg cgcgtacgtc
     3421 cttagtcctc cgcttcacgc tacttcttca gcacctcgtc atccggttgc acgcccagca
     3481 cggcaccctt tgccttcatc cacgccgcca gctcgtcgtg ttttccgcgc attgcctcgg
     3541 ccagcggagt cctccgcttt cacgtaggtc ggactctcca gtccgacaga acgagagtcg
     3601 gactggagag tccgacctac gttgtccgcg gctggacgcc ggggttcccc tgcgattgcc
     3661 gaagagtgct taagtcaagg cttggacacg atccgtgacg cgcgtacgtc cttagtcctc
     3721 cgcttcacgc tacttcttca gcacctcgtc atccgg
//
LOCUS       DATLVW010000163        10614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_253861, whole genome shotgun sequence.
ACCESSION   DATLVW010000163 DATLVW010000000
VERSION     DATLVW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253861"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VNN25_05350"
     CDS             <1..1506
                     /locus_tag="VNN25_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HXH90984.1"
                     /translation="PFGPRARKDAALYLGFDSPLQFTDQQVNLMFYVRTEEEKGSTHC
                     DVDLSTMPLPATIVWEYWTGTVWQHFELDKDDTRAFTRSGHVLFPGPGSKFKKLAAGE
                     VKTALYWIRARLTAPTYEKAPELEAALTNTTTARQATTIRDEILGDSDGRPEQKFTLA
                     SSPVVDRDRAERAVTKNGVVTITSVRLEIDEGLGFELWQEVDDFYASGPGDMHFRCNH
                     TTGEILFGDGDHGRIPTANPTGEPNVIAREYRSGGGRAGNVGAGQITAMQTSVDGVAS
                     VTNMRAALGGSDEEGLDDAKLRAPMALKSKNRAVTAEDFEYLAMATPGVRLRRAKALP
                     LYHPEFNTAPIPGVVTVVIVPDSDSPKPSPGEATRRIVCEHLNQHRLLTSEVCVIAPA
                     YRLVLIEADIVVAASADLSQVKRQVEDALTRYFHPINGGDEGLGWPFGGEIFYSSVYR
                     VLLQVEGVVRIRENQMLIWLDGERQTFCRDVAINIGELLWTDGHDIRVAYA"
     gene            1499..3538
                     /locus_tag="VNN25_05355"
     CDS             1499..3538
                     /locus_tag="VNN25_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HXH90985.1"
                     /translation="MRNGRESLLLNELAGWRAASLTATLELAESRLRLRRQPGSLRPL
                     VDAGGSFGGLTYPSGLAVDADGRIYVIDGRTIKRFDPCTGKFTTLPCVLLDKPRGIAI
                     SPSDDLFVTDGDRVLVYLLPSLKLLRVITRRKWEPWDVAVTLDCRVFISDYAGGVVWR
                     VRRHDLVPVVEALVKPTAIAVDAQCNLYVIQEGKKEVVVFDEKGKLVRTITVADEAKD
                     AFCPTGIAVDANGNVCIADLADRTVYTTASGCDEARPVRAVDAAPGGLAFDARGNPIV
                     AEADRKRICTLEAKAAYETEGTFVSEALDSRIYRCQWHRVRLHRALPAGAQIYVETFS
                     SESLKTDEEIAGLPDSRWLTREYDAQVSEGEWDCLVQSPPGRYLWLRLTLIGNGGVTP
                     EIGKVRVHFPRASSMQYLPAVFGENAVARDFVARFLSIFDTIRDGISDNVRDAAAYFD
                     PEATPAGFLDWLASWIGLALERNWPVAKRRMLVRDAHKLYEKRGTPEGLRMHIRLFAG
                     REPLLLEHFQLRRWMFLNRGRLGDCSALFGASIVRRLQLDRFSRIGEFQLVDSGDPLR
                     DPFLVHAHQFSVMVPLRRGADAESERRALERIIEASKPAHTKAELHLIEPRLRIGTQA
                     FIGIDTVVGEYPSGVTTGKGNLGRDTVLGPPTDESRPPAMRIGARSRIGVSTSLR"
     gene            3555..6224
                     /locus_tag="VNN25_05360"
     CDS             3555..6224
                     /locus_tag="VNN25_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXH90986.1"
                     /translation="MSDCTQHGSGHANGCIECEVPQLARNHYFTGKLLVERDFTDEQR
                     YFVGKDRRHNRFLHGRGTVCGLKVVEHPNPACRSKYVIVKPGLAIDCCGREVIVCRDE
                     TFDFRSRVEEILLANAPEPAEGEEKEPDDRPHTVQICIKYRECGDEEIPALFDECGCD
                     DGGCQPNRILETFAPDVIVDPEAPKKDVAGGLSLEWKNTIAATFANKVVLDEANKKLW
                     VLGDFPLLKKGFSSKMFVFNLDNLSPISVTELSAYEFDLSQDGKLLYLAEDHQIGIYD
                     ATTFGAGAVPIKTIATATRTVALAASGDKSIYAIINGKVIAWDGTTETEISIGGPMDL
                     VVTTDGKWLFVAEAAEQKVLMIDTSKLTDPPVEFDATAPWRLAVETTTDGMRLAVADN
                     SAQQLRLYSVKPGDVKPFVQLGDPYPLPTGGNPPDLQFSGGARWVYVPGVSEPKLFAM
                     DAHAIELKKPQPSLSIEVGLSPHNIAMRGDGRKLFVAFMGIDPGLGGPDTTTPAAGGV
                     AVIDVTEEDCRSIFDQIIEPCPECGGDDCLVLATIEKYVFGDSLTEANIDNLRDRPLL
                     PSTSVITEVVECILEHGGTGGVGPQGPPGQPGATGPTGLTGPTGLTGPTGPTGPTGLT
                     GPTGHDGKNAVPLDLPRIVAINWVHDQTYLPNAGDFGKLWSRGLVIAFDRPMLAETLP
                     RSIELLIRRSESDDIYKFNRLQCWCAIPGMIEGVHIEAPACGKVPAAVGSPIPSGPVN
                     GVRFRPINANGAFVTDMPMAEYLVLLYGDQILGDAKIEVEGKMVHPALDANHLSPYLT
                     GIPAVHALGTRCPTGNLTEGGTFRSWFRYGVNINDVNPKDLGEIVGNEAVAKAIIDFR
                     TKNGFINSIEDLAQVKGVGAATMTKLRENASVS"
     gene            6237..8135
                     /locus_tag="VNN25_05365"
     CDS             6237..8135
                     /locus_tag="VNN25_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HXH90987.1"
                     /translation="MASPKPVILPRQKPYYVPLNTSRKTAAQCCQCPACLGLECLERP
                     RYFAGQLLTEAELNSEQSYVIAKNRLHNRYLHGWGVVCGLEVVCHNCDGFVTVRAGYA
                     LDPCGNDVIVCKDTELNILDAIQKCVDARRKSNDDCPPWRDPNDTCSDDDQHWCVTLQ
                     YVENEGRSVMPLQETKSHCGCGGKCGGGGGCGCGGNGNSKKNGGCSCGATKAATPKKT
                     ACEPTRIFEQYRLGIIPEPDECASRRQMKSDWTTVTKRYEYSDLAKVKVGANVGLFSI
                     QSLIPGSIRKHPLFPALVAAAPDDSIFMRAIALAQELEDFLAQRFSARELEMLRAIGL
                     RCRYEQAYRSDEHKKTLADLRAISVALEAFNTDRNSYPVEVRDFEDLRDTLSPTYMKE
                     VPQTDGWGTAYHVVVTKDKYRIISAGEDRKFDASSERMDAPPNEDIVIQNSVIVWTGN
                     STTYDDSSPAYQLCCKFRRAIKDLYAENPLNVRCVPFDCPPCAEDPTNSNDERQIGAS
                     DEPASTPNNPMCCLIDALTNYYIDALCMLFLPGCPKPPCEDRVIIACVTVQKGKIVHI
                     CNHACRHYAGSFNSVQHWMSVLPVIPLIGAILRDVCCSENGFDVLLGLSGSSRAQLRR
                     TEEGVLHA"
     gene            8146..10026
                     /locus_tag="VNN25_05370"
     CDS             8146..10026
                     /locus_tag="VNN25_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90988.1"
                     /translation="MAEVIERPNYYQLQFLGAEDFKSEQAYHRDMRRRHNIGPHRWGI
                     VVGLELVEKPRDSGTGFDVYIQAGMAIDGFGREIFVAAPTKLDSADFQFVSGTLPSLH
                     EVWIAYREEEALPPEFGYGTCESDGSNNRVREDFRIVIDPAGDKHAAIVVDGRPADPS
                     TIPDDESVPYQELPDSAAARWLVRIGTVNWDPQAKEFVTITDGSDPAKEREQLLQGRV
                     YAGIVAEEVLGPNQKLRLRPRAAFAATDIDKSDFAFVEGRMRVKGSIAEEKDIFLEGG
                     KISFQNAAGSDANAALWIQRMGGNSNAWDLRVHIGADTDHEKHRLTVGTGSDPIALST
                     EKVFFAVRGDDRVEIPTGTLYFGETKRQMMTLYAATDDAEPPYGIGVQDSSLYFRTGG
                     HVYWYRGGAHEDGQGKPGGTGVELLHLDDKGCLSFGSKTRQMLNLWSTSYGIGVQSAT
                     LYFRSAFDFRWFRGGSHGDGRGDTNGGTLVMSLDDSGSLFLEGDAVIGRSLTVSGGLA
                     IDGNLVMAKGKAMILDGTRFPIDIRTGEVGFSTGGATSGQHTFSVFSRLPKITGGAFI
                     HVALADIKHDGTVSAPRWSVSAHTTNTQTNDNTFNFTFDWIVDSTHSTINLVSYVAFF
                     IA"
     gene            10040..10237
                     /locus_tag="VNN25_05375"
     CDS             10040..10237
                     /locus_tag="VNN25_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002786697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90989.1"
                     /translation="MFEQRIEELQKELAKGEQQLASLDRERALTRDTILRIQGAIQVL
                     EELRATEPPAESRPVLAHTSS"
     gene            10234..>10614
                     /locus_tag="VNN25_05380"
     CDS             10234..>10614
                     /locus_tag="VNN25_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90990.1"
                     /translation="MIESTARFTITRAGCTYIVPREHPAPDDVRRRLDGVLRDRVLTP
                     DVLDALGPEADPAVWLIERLDVGVAIFDALEDDRVAELWGREIARAVAHTVRRGAGVL
                     RFADRAAFIAQYVADAAAGRDGGKW"
BASE COUNT         2038 a         3450 c         3410 g         1716 t
ORIGIN      
        1 ccgttcggtc cgcgcgcgcg caaggacgcc gcgctctacc ttggattcga ttcgcccctg
       61 cagttcaccg accagcaggt gaacctgatg ttctacgtcc gcaccgaaga agagaaagga
      121 agcacgcact gcgacgtcga cctgtccacg atgccgctcc ccgccacgat cgtatgggag
      181 tactggaccg gcacggtgtg gcagcacttc gagctcgaca aagacgatac gcgcgccttc
      241 acgcgcagcg gccacgttct cttccccgga ccgggatcga agttcaagaa gctcgcggct
      301 ggtgaagtga agacggcgct ctactggatc cgcgcgcgcc tcaccgcgcc gacgtatgag
      361 aaagcaccgg agctcgaggc agcgctcacg aacacgacca cggcgcggca ggcgaccacc
      421 atccgcgacg agatcctcgg cgacagcgac ggccgtcccg aacagaagtt cacgctcgct
      481 agctcgcccg tcgtcgatcg cgaccgcgcc gagcgcgccg tcacgaagaa cggcgtcgtc
      541 acgatcacca gcgtgcggct ggagatcgac gaaggactcg gcttcgagct ctggcaggaa
      601 gtcgacgact tctacgcctc gggtccgggc gacatgcact tccgctgcaa tcacaccacc
      661 ggcgagatcc tcttcggcga cggcgatcac ggtcgcatcc cgaccgccaa tccgaccggc
      721 gagccgaacg tgatcgcgcg cgagtaccgc tccggcggcg gacgcgcggg caacgtcggc
      781 gcgggacaga tcacggcgat gcagaccagc gtcgacggcg tggcgtcggt gacgaacatg
      841 cgcgccgcgc tcggcggatc ggatgaagaa gggctcgacg acgcgaagct ccgtgcgccg
      901 atggctctga agagcaagaa ccgcgcggtc acggcggagg acttcgagta cctggccatg
      961 gctacgccgg gtgtgcgcct ccgccgcgcg aaagcgctgc cgctctatca ccccgagttc
     1021 aacaccgcac cgattccggg cgtcgtgacg gtggtgatcg tgcccgacag cgattcgccg
     1081 aaaccgtcgc ccggcgaagc gacgcggcgc atcgtctgcg agcacctcaa tcagcatcga
     1141 ctcctgacgt ccgaggtctg tgtgatcgcg ccggcctacc ggctggtgct gatcgaggcc
     1201 gacatcgtcg tcgccgccag cgccgatctc tctcaggtca aacgccaggt cgaagacgcg
     1261 ctgacgcgct acttccatcc gatcaatgga ggggacgagg gactgggctg gccgttcggc
     1321 ggcgagatct tttactcgag cgtttatcgc gtgctcctgc aggtcgaagg agtggtgcgc
     1381 attcgcgaga accagatgct gatctggctc gacggcgaac ggcagacgtt ctgccgcgac
     1441 gtggccatca acatcggcga actgctctgg actgacgggc acgacatccg ggtggcgtat
     1501 gcgtaacgga cgcgagagcc tgctgctcaa cgagctcgcc ggatggcggg ccgcgtcgct
     1561 cactgcgacg ctggagctgg cggagtcgcg ccttcgtctc cgccgccagc cgggatcgct
     1621 ccgcccgctc gtcgacgccg gcggctcgtt cggcggactg acctatccga gcggcctggc
     1681 cgtcgatgcg gacggtcgga tctacgtcat cgacggcaga acgatcaaac gctttgatcc
     1741 ctgcaccggc aagttcacga cgttgccgtg cgtgctactc gacaagccgc gcgggatcgc
     1801 gatctccccc tcggacgacc tcttcgtgac cgacggtgat cgcgtgctcg tctacctcct
     1861 gccgtcgtta aagctgctgc gcgtgatcac ccgccggaag tgggagccgt gggatgtcgc
     1921 ggtcacgctg gactgccgcg tgttcatcag tgattacgcg ggcggcgtgg tctggcgtgt
     1981 acgacgacat gacctggtgc ctgtcgtaga agcgctcgtc aaaccgactg ccatagccgt
     2041 cgatgcgcaa tgcaacctct acgtcatcca ggagggtaag aaggaagtcg tcgtcttcga
     2101 cgagaaggga aaactggtcc gcacgattac ggtggccgat gaggcgaagg acgcgttctg
     2161 tcccaccggc atcgcggtcg acgcgaacgg caacgtctgc atcgccgatc tggcggaccg
     2221 aacggtctac acgactgcga gcggatgcga cgaagcgcgg cctgtacgtg cggtcgacgc
     2281 cgcgccgggc gggctggcgt tcgacgcgcg cggcaatccg atcgtcgccg aggccgatcg
     2341 caagcgtatt tgcacactgg aggcgaaggc ggcttacgag accgaaggga cattcgtctc
     2401 tgaagcgctc gacagccgga tctatcgctg ccagtggcat cgcgtgcggt tgcatcgcgc
     2461 gctgccggcc ggcgcgcaga tctacgtcga gacgttcagc tcggaatcgc tgaagacgga
     2521 cgaagagatc gccggcctcc ccgactcgcg ctggctaacg cgcgaatacg acgcgcaggt
     2581 cagcgagggc gaatgggatt gcctcgttca gagtccaccg ggacgttacc tctggctgcg
     2641 cctgacgctg atcgggaacg gtggcgtgac gccggagatc ggcaaagtgc gcgtgcactt
     2701 cccgcgtgcg tcgtcgatgc agtatctccc cgcagtgttc ggcgaaaacg cggtggcgcg
     2761 cgatttcgtg gcccgcttcc tctcgatctt cgacacgatc cgcgacggta tctcggacaa
     2821 cgtgcgcgat gcggcggcgt acttcgaccc cgaggccacg ccggccggat ttctcgactg
     2881 gctggcgtca tggatcggcc tggcgctgga gcgcaactgg ccggtggcga agcggcgcat
     2941 gctcgtgcgc gacgcgcaca agctttacga aaaacgcggc acgcccgagg ggctgcggat
     3001 gcacatccgc ctcttcgccg gccgcgagcc gcttctcctc gagcacttcc agctgcgacg
     3061 ctggatgttt ctcaaccgcg gccggctggg cgattgctcc gcgctcttcg gcgcgtcgat
     3121 cgtgcgccgg ttgcagctcg accgcttcag caggatcggc gagttccagc tcgtcgacag
     3181 cggcgatccg ctgcgcgatc cgttcctcgt ccacgctcat cagttctccg tgatggtgcc
     3241 gctccgccgc ggcgccgacg ctgagtcgga gcggcgcgcg ctggaacgaa tcatcgaggc
     3301 gtcgaagcca gcgcacacca aagcggaact gcacctcatc gaaccacgcc tgcgcatcgg
     3361 cacgcaggcg ttcatcggaa tcgacacggt cgtcggtgaa tacccgtccg gtgtgaccac
     3421 cggcaaggga aatctcggcc gcgacaccgt gctcgggcct cccacggacg aatcgagacc
     3481 accggcaatg cgcatcggcg cgcggagcag gatcggcgtc agcacgtcgc tgcgctgacc
     3541 ggaaggagtg aagcatgtcg gactgcacac agcacggatc agggcacgcc aacggttgca
     3601 tcgaatgcga agtgccgcaa ctcgcgcgca accactactt caccgggaag ctgctggtcg
     3661 agcgggactt caccgacgag cagcgctact tcgtcggcaa agaccgccgc cacaaccgct
     3721 tcctccatgg acgcggcacg gtctgcggtc tgaaggtggt cgagcatccc aatcccgcct
     3781 gccgctcgaa gtacgtgatc gtgaagccgg gactggcgat cgactgctgc ggccgcgagg
     3841 tgatcgtctg ccgcgacgag acgttcgact ttcgctcgcg cgtcgaggag atcctcctgg
     3901 ccaacgctcc ggagccggcc gaaggggagg agaaggagcc ggacgacaga ccgcacaccg
     3961 tgcagatctg catcaagtac cgcgaatgcg gcgacgaaga gatcccggcc ctcttcgacg
     4021 aatgcggatg cgatgacggc ggctgtcagc ccaatcgcat cctggagacg ttcgcgccgg
     4081 atgtgatcgt cgatcccgag gcgccgaaga aggacgtggc cggcggcctg tcgctggagt
     4141 ggaagaacac catcgccgcc acgttcgcga acaaggtcgt tctcgacgag gcgaacaaga
     4201 agctctgggt cctcggcgat tttccgttgt tgaagaaggg cttctcctcg aagatgttcg
     4261 tcttcaacct cgacaacttg agcccgatct cggtgacgga gcttagcgcg tacgaattcg
     4321 atctctcgca ggacggcaag ctgctctatc tcgctgaaga tcaccagatc gggatctacg
     4381 acgccaccac attcggcgcc ggcgcggtgc cgatcaagac gatcgccacc gccacacgca
     4441 cggtcgcgct cgcggcatcg ggcgacaagt cgatctacgc catcatcaat gggaaggtga
     4501 tcgcatggga cggcacgaca gaaaccgaga tcagcatcgg cggcccgatg gacctcgtcg
     4561 tcaccaccga cggcaagtgg ctgttcgtcg ccgaagcggc cgaacagaag gtcctgatga
     4621 tcgacacatc gaagctcacc gatccgccgg tggagttcga cgcgaccgcg ccgtggcggc
     4681 tggcggtgga gacgacgacc gacggaatgc ggctcgcggt ggccgacaac agcgcgcagc
     4741 agctccggct ttatagcgtg aaaccgggcg acgtgaagcc gttcgtgcag ctcggcgatc
     4801 cgtacccatt accgactggc ggcaatccac ccgatctgca gttctccggc ggcgcgcgct
     4861 gggtctacgt gcccggcgtc agcgagccga agctcttcgc catggacgcg catgcgatcg
     4921 agttgaaaaa gccgcagcct tcgctctcca tcgaggtcgg cctctcgcct cacaacatcg
     4981 ccatgcgcgg cgacggacgc aagctcttcg tggcattcat gggcatcgat ccggggctgg
     5041 gcgggcccga tacgacgacc cctgcggcgg gcggcgtggc ggtcatcgac gtcaccgagg
     5101 aggactgccg gtcgatcttc gatcaaatca tcgagccgtg tcccgaatgc ggcggcgacg
     5161 actgcctcgt gctggccacg atcgagaagt acgtgttcgg cgactcgctc accgaagcga
     5221 acatcgacaa cctccgcgac cgtccgttgc tgccgtcgac gagcgtgatc accgaggtcg
     5281 tggaatgcat cctcgaacat ggaggtaccg gcggcgtcgg tccgcaaggt ccgccgggac
     5341 aaccgggagc gaccgggcca acgggactga ctggaccgac cggactaacg ggaccaacgg
     5401 gaccaacggg accaacggga ctgactggac caacgggaca cgacgggaag aacgcggtcc
     5461 cgctcgatct gccgcgcatc gtggccatca actgggtcca cgatcagacc tacctcccca
     5521 acgccggcga ctttgggaaa ctctggagcc gcggacttgt gatcgcgttc gacaggccga
     5581 tgctggcgga gacgctgccg cgctcgatcg agctgctcat acgccgctct gagagtgacg
     5641 acatctacaa attcaatcgc ctgcagtgct ggtgcgcgat tcccgggatg atcgagggtg
     5701 tgcatatcga ggcgcccgcg tgcggcaagg ttccggccgc tgttggatct cccatcccga
     5761 gtggtccggt caacggcgtc cgtttccgtc cgatcaatgc gaacggagcc ttcgtgacgg
     5821 acatgccgat ggccgagtac ctcgtcctcc tctacggcga tcagatcctc ggcgatgcga
     5881 agatcgaagt cgagggaaag atggtccatc ccgcgctgga tgcgaatcac ctttcgccgt
     5941 acctcaccgg aatcccggcg gtgcatgcac tcggcacacg ctgcccgacg ggcaacctca
     6001 ccgaaggggg cacgttccgc agttggttcc gctacggggt gaacatcaac gacgtgaatc
     6061 cgaaggatct cggggagatc gtcggcaacg aggccgtggc caaagcgatc atcgattttc
     6121 gcacgaagaa cggcttcatc aactccatcg aagacctcgc gcaggtcaag ggcgtcggcg
     6181 ccgcgacgat gaccaagctg cgcgaaaacg cgtcggtgag ttagcaggag gacgagatgg
     6241 catcaccgaa accagtgata ctacccaggc agaaaccgta ctacgtgccg ctgaacactt
     6301 cgcgcaagac ggcggcgcaa tgctgtcaat gtccggcctg tctcggactg gaatgcctcg
     6361 aacgcccgcg ctacttcgcc ggccagttgc tcaccgaggc agagctgaac agcgaacagt
     6421 cgtacgtcat cgccaagaat cgattgcaca accgctatct gcacggatgg ggcgtcgtct
     6481 gcggactcga agtcgtgtgc cacaactgcg atggtttcgt caccgtgcgc gccggttatg
     6541 ccctcgatcc gtgcggcaac gacgtcatcg tctgcaagga cacggagctg aacatcctcg
     6601 atgccattca gaaatgcgtc gatgcccgac ggaagagcaa tgacgactgc ccgccgtggc
     6661 gcgatccgaa cgacacctgt tccgacgacg accagcactg gtgcgtcacg ctgcagtacg
     6721 tcgagaacga agggcgttcc gtcatgccgc tgcaggagac aaagagccat tgcggctgcg
     6781 gcgggaagtg cggaggcgga ggcgggtgcg gctgcggtgg aaacggcaac agcaagaaga
     6841 acggcggatg ctcgtgcggc gcgacgaagg cggcaacgcc gaagaaaacc gcatgcgagc
     6901 cgacgcgcat cttcgagcag taccgcctcg gcatcatccc ggagcccgac gaatgcgcga
     6961 gccggagaca gatgaagagc gactggacca cggtcaccaa gcgctacgag tactccgacc
     7021 tcgcgaaggt gaaggtcggg gcgaacgtcg gattgttcag catccagagt ctcatcccgg
     7081 gaagcatccg caagcatccg ctcttccccg cgctggtcgc ggctgcgccc gacgactcga
     7141 tcttcatgcg cgcgatcgcc ctggcgcagg agctcgagga tttcctcgcc cagcgcttca
     7201 gcgcgagaga actggagatg ctgcgagcga tcggcttgcg ctgccggtac gaacaggcct
     7261 atcgcagtga cgagcacaag aagacgctgg ctgacctccg ggcgatatcc gtcgcactcg
     7321 aagcgttcaa tacggaccgc aactcctatc ccgtcgaagt gcgggatttc gaggacctca
     7381 gggataccct tagcccgacg tacatgaaag aggttcccca gaccgacggc tggggcacgg
     7441 cctaccacgt cgtggtcacc aaagacaaat accggatcat cagcgccggc gaggacagga
     7501 agttcgacgc cagctccgag aggatggacg cgccgccgaa cgaggacatc gtcatccaga
     7561 acagcgtcat cgtctggacc ggaaactcga cgacgtacga cgactcctcg ccggcgtatc
     7621 agctctgctg caagttccgc cgtgcgatca aggatctcta cgccgagaac ccgctcaacg
     7681 tccgttgcgt cccgttcgat tgtccaccct gcgccgaaga ccccacgaat tcgaatgatg
     7741 aacgacagat cggtgccagc gacgaaccgg cgagcacgcc gaacaacccg atgtgctgcc
     7801 tgatcgacgc gctgacgaac tactacatcg acgcgctctg catgctcttt ctccccggat
     7861 gcccgaagcc gccctgcgag gaccgcgtga tcatcgcctg cgtcaccgtg cagaagggaa
     7921 agatcgtcca catctgcaac cacgcctgcc gccattacgc cggcagcttc aactcggtgc
     7981 agcactggat gtcggtactg ccggtgatcc cgctgatcgg cgcgatcctg cgcgacgtct
     8041 gctgctcgga gaacggcttc gacgtcctcc tcggtctttc gggcagctcg agagcgcaac
     8101 tgcggcggac ggaagaaggc gtcctgcacg cgtgaggaac atgccatggc cgaagtgatc
     8161 gaacgtccca actattacca gcttcagttc cttggcgcgg aggatttcaa atccgagcag
     8221 gcctatcacc gcgacatgcg gcggcggcac aatatcggtc ctcaccgctg gggcatcgtc
     8281 gtcgggctgg aactggtaga gaaaccgcgc gacagcggca ccggcttcga cgtctatatc
     8341 caggccggca tggccatcga tggattcggc cgcgagatct tcgtcgccgc gccgacgaag
     8401 ctcgacagcg ccgacttcca gttcgtcagc ggcacgctgc cgtcgctgca cgaagtctgg
     8461 atcgcctatc gcgaggagga agcgctgccg ccggagttcg gctacggcac gtgcgagagc
     8521 gacgggagca acaaccgcgt acgcgaggac ttccgcatcg tcatcgatcc tgccggcgac
     8581 aaacacgcgg cgatcgtcgt ggatggacgt ccggccgatc cgtcgacgat tccggacgat
     8641 gagtcggtgc cgtatcagga gctccccgac tccgcggccg cgcgctggct ggtgcgcatc
     8701 ggcaccgtga actgggatcc gcaggcgaag gagttcgtga ccatcaccga cggatcggat
     8761 ccggcgaaag agcgcgagca gcttctccag ggccgcgtct atgccggcat cgtggccgag
     8821 gaggtgctgg ggccgaatca gaaactccgg ctccgaccgc gtgcggcgtt cgccgccacc
     8881 gacatcgaca aaagcgattt cgctttcgtc gaagggcgga tgcgcgtgaa ggggagcatc
     8941 gccgaggaga aagacatctt cctcgaaggg gggaagattt ccttccagaa cgcggccggc
     9001 agcgatgcca acgcggctct ctggatccag agaatgggcg gcaactcgaa cgcctgggac
     9061 ctgcgagtcc acatcggcgc cgacaccgac cacgagaaac atcgccttac cgtcggcaca
     9121 ggaagcgatc ccatcgcact gtcgacggag aaggtcttct tcgccgtacg cggcgacgat
     9181 cgcgtggaga ttccgaccgg cacgctctac ttcggcgaaa cgaagcggca gatgatgacg
     9241 ctctacgcgg caaccgacga cgccgagccg ccgtacggca tcggcgtgca ggactcctct
     9301 ctctactttc gcacgggcgg gcatgtgtac tggtaccgcg gcggcgccca cgaagacggc
     9361 caggggaagc cgggcggcac cggcgtcgag ttgctgcacc tcgacgacaa gggctgcctc
     9421 tccttcggct cgaaaacccg gcagatgctg aatctctgga gcaccagcta cggcatcggc
     9481 gtgcagtccg cgacgcttta tttccgcagc gcgtttgact tccgctggtt ccgcggtggc
     9541 tcgcacggcg acggacgcgg cgacacgaac ggaggcacgc tggtgatgtc actcgatgac
     9601 tcgggtagcc tctttctcga gggcgacgcc gtcattgggc gcagcctgac cgtgtcgggt
     9661 ggcctggcga tcgacggcaa cctggtgatg gctaaaggga aagcgatgat cctcgacgga
     9721 acgcgcttcc ccatcgacat ccgcaccggt gaagtggggt tcagcaccgg cggtgcgact
     9781 tcgggtcaac acacgttctc cgtgttcagc cggcttccca agatcaccgg cggcgcgttc
     9841 attcatgtgg ccctcgccga catcaaacac gacggaaccg tgagcgctcc gcggtggagc
     9901 gtgtcggctc ataccaccaa cacgcaaaca aacgacaaca ccttcaactt caccttcgac
     9961 tggatagtcg acagcacgca ctcgacgatc aatctcgtgt cgtacgtcgc cttcttcatc
    10021 gcctgaggag tgatcggcca tgttcgaaca acgcatcgaa gagcttcaga aggaactggc
    10081 gaagggagag cagcaactgg cctcgctcga ccgcgagcgc gccctgacgc gggacacgat
    10141 tctgcgcatc cagggcgcga tccaggtgct cgaggagttg cgcgccacgg agcctcccgc
    10201 tgaatcgcgg ccggtcctgg cgcatacgtc gtcatgatcg agtccaccgc gcggttcacc
    10261 atcacccgtg cgggctgcac ctacatcgtt ccgcgcgaac atcccgcgcc ggacgacgtg
    10321 cgccggcgcc tggatggagt tctgcgcgac cgggtgttga cgccggacgt cctcgatgcg
    10381 ctcgggcccg aggccgatcc ggcggtctgg ctgatcgaac gcctcgacgt cggcgtggcc
    10441 atcttcgacg ctctcgagga cgacagggtc gcggagctgt ggggacgcga gatcgcgcgc
    10501 gccgtcgccc acacggtgcg gcggggcgcc ggcgtcctgc gcttcgccga tcgcgccgcg
    10561 ttcatcgcgc aatacgtcgc cgatgccgcc gcgggccgcg acggcggcaa gtgg
//
LOCUS       DATLVW010000164         4793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_377806, whole genome shotgun sequence.
ACCESSION   DATLVW010000164 DATLVW010000000
VERSION     DATLVW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377806"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(703..1671)
                     /locus_tag="VNN25_05385"
     CDS             complement(703..1671)
                     /locus_tag="VNN25_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXH90991.1"
                     /translation="MTKLLILSEHDVERLLTMRECTEVMEEALSALARGEVHNPLRSM
                     IRGEGAKGILGLMPSYRGGPQPHYGLKEVCVFPGNPAIGLDTHLGGVLLHSGETGQLL
                     AVMNASAITAIRTAAVSAVATKLLAREDARTLAIIGAGVQARTHLEAIPLVRNINDVR
                     IVSRTPAKAAALAGKSARVVASVEEAVRGADIIVTATSSREPILKREWLADGVHINAV
                     GSSIAVARELDGATVAASSLFVDRRESTINEAGDYLFALREGAISDGHIRAEIGDILI
                     GKAKGRSSDDEITLFKSLGLAVEDLASAQFLFEKASREKAGSWVDF"
     gene            complement(1668..3110)
                     /locus_tag="VNN25_05390"
     CDS             complement(1668..3110)
                     /locus_tag="VNN25_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90992.1"
                     /translation="MNGNWDPRTLVTARSASRLFPLLLLAAFVAVVSFVAFHHEPWRD
                     EADPWLYVRDAGLMTILARTRYAGLPALWFLCLAPLAKLGLAYASQKVLHLAIAAAGV
                     WLLATRSPLSRLTATLLTFSYYIAYEYSVVVRSYALTIMLTFAAAALYRRSRERPIAF
                     ALVLLLLFNCNAQGFFIAGTFAALFVIERIERRQIGARDAIAAALMAASALLAWFQVR
                     TPPDPARQAVMRVFNRDAFAWTVSNAFLPTIPTSVGFLIGVAVLLLITFAIRRSREAV
                     LFLWLPLSVLAILYSYVWLGGLRHAGFFLLLTIVAIWIAGDRDGELIDRRWAAAAAIA
                     LNLSLLVSMFVAMQYWVADIREPFSGAQEMAAFIRDQKLDRIPIAAHNLTQCEALLPW
                     LPGTRFWYAGLGEEGTYMKWDAALERALNVPYPAAERRSQEHFQGRPWLLLFNVEMPD
                     PAAHGFRLLHATAEPFEKTDERYWLYAPTR"
     gene            complement(3107..4018)
                     /locus_tag="VNN25_05395"
     CDS             complement(3107..4018)
                     /locus_tag="VNN25_05395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH90993.1"
                     /translation="MRHRFDSVVDGFAILGCDRCGSARTWPALDETAIGSWYPKAYYG
                     ERNVRFNPLFERLTRWFRRRRVDVIRRLTRPGAVLDVGCGRGFTLHFLREAGYQPVGV
                     ELSPTAASHARDVLELDVRTGDFLSQTFESDEFKAVVFWHSLEHMIDPLQALDRAWEI
                     LEPGGLLVIAVPNFVSIQAAWTGRQWFHLDVPRHYVHFSTRGLTDALRRRGFRMTSVA
                     HFSLEQNPYGWIQSLLNRVFEHDLLYSILKEPAARAHRIREFPLQTIATTIGAIFLLP
                     VAFVLMMLETLLRRGGTIEVYARKPER"
     gene            complement(4072..4434)
                     /locus_tag="VNN25_05400"
     CDS             complement(4072..4434)
                     /locus_tag="VNN25_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH90994.1"
                     /translation="MPTHIKQPSVIEAAGNKPKRIEEYIGRVNSKSAGISVARMQSPS
                     GWVEPGQTPEFEEYTVVLRGSLRVTTREGVIDVGAGEAIITRSGEWVQYSTPGAEGAE
                     YIAVCLPAFSMDSVHRDA"
     gene            complement(4557..>4793)
                     /locus_tag="VNN25_05405"
     CDS             complement(4557..>4793)
                     /locus_tag="VNN25_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH90995.1"
                     /translation="GNKAASAIRASVDDDPDSAVRDKAVFAVSQLPDDESVPMLIELM
                     KSHRDPSVRKKAAFWLGQKHDPRALAAIEDILRK"
BASE COUNT          941 a         1564 c         1478 g          810 t
ORIGIN      
        1 gccgtttctc aatgatttgc agtccacaaa cgaccaaagg cctggcccca ctggtgcttg
       61 acgcgatcgc ggttacttcg cgagacaact tcgtagaatc gaatatctaa cgctccgacg
      121 ttcgctgcaa cgcggaagtc actcaaagct cgctgctggt caagaatatc tgagcgtcag
      181 acattcgcaa cgtccaagga cccgcgaatg tctaacggcc agacgttcgc gatgacgcgg
      241 tagcttcgaa tgtctgacgc tcagacgttc gttgcaacgc ggaagtcatt caaagcccgc
      301 cgctggtcat gaatatctga gcgtcagaca ttcgcagtgt cggcggagcc gcgaatgtct
      361 gacggccaga cgttcgcgac gacacggtca cctcgcgaga cgatttcgta gaatcgaata
      421 tctgacactc agacgttcga tacaacatgg aagtcattca aagcccgccg cgagtcatga
      481 acctccgagc gcccgacact cgcagagtcg ccggagccgc gaacgaccgt cacgcagaca
      541 ctcgcggtgc cgcggcagcc gttcgaagct ggcttcgagc agcgaataat gaccttcaaa
      601 tactcacgcc ccctggcccg caatgaacag cacgatcatc acgaacgtga ggatcgccat
      661 gacgagccag cttggccgcg ctgcgattgc tctcagtcct agctagaaat caacccagct
      721 ccccgccttc tcgcgtgagg ccttctcgaa caggaattgc gccgatgcga ggtcttcgac
      781 ggccaacccg agcgacttga agagggtgat ctcatcgtcg gacgagcgcc ccttggcctt
      841 tccgatcagg atgtctccga tctcggctcg gatgtgaccg tcgctgatcg ctccttcccg
      901 gagagcgaag aggtagtcgc cggcctcgtt gatcgtcgat tcacgccggt cgacaaagag
      961 agatgaggcc gcaaccgttg cgccatcgag ctcgcgggcg accgcgatgc tggagccaac
     1021 cgcattaatg tggacgccgt ccgcgagcca ttcacgcttc aggatcggct cgcgggagct
     1081 ggtcgcggtc acgatgatgt ccgcgcctcg tacggcttct tcgaccgagg cgacgacgcg
     1141 ggcactcttc cctgcgaggg cggcagcctt cgcgggcgtc cggctgacga tgcgcacgtc
     1201 attaatgttg cgcaccagcg ggatggcctc gaggtgtgtt cgtgcctgaa cgccggcgcc
     1261 gatgatggcc aacgtccgag cgtcttcgcg ggcgagcagt ttcgtggcca cggcggacac
     1321 cgcggcggtc cggatcgcgg tgatcgccga tgcgttcatg actgctagaa gttggccggt
     1381 ctcaccactg tggagtagga ccccgccgag gtgggtgtcg agaccgatgg cagggttgcc
     1441 ggggaagacg cagacttcct tgaggccgta gtgcggttgc ggtccgcccc gatacgacgg
     1501 catcagaccc agaatgccct tcgctccttc gccacggatc atgcttcgca gcgggttgtg
     1561 aacctcgcct cgggccagcg cgctcagggc ttcttccatc acctcggtgc actcgcgcat
     1621 cgtgaggaga cgctcgacgt cgtgttcgct gaggatgagt agctttgtca tcgtgtggga
     1681 gcgtacagcc aatagcgctc gtctgtcttc tcgaatggtt cggcggtcgc gtgcaggagc
     1741 cggaagccgt gcgcggccgg gtccggcatc tcaacattga atagcagcag ccagggccgc
     1801 ccttgaaaat gctcctgcga acgcctttca gcggccggat acggaacatt gagagcacgc
     1861 tccaacgccg cgtcccactt catgtaggtt ccctcttccc cgagtccggc gtaccagaac
     1921 cgggtgccgg gcaaccaggg cagaagcgct tcgcactgcg tgaggttgtg cgccgcaatc
     1981 gggatgcggt cgagcttctg atcgcggatg aacgcagcca tctcctgcgc tcccgagaac
     2041 ggctcgcgaa tgtcggcgac ccagtactgc atcgccacaa acatcgagac gagcaggctg
     2101 aggttcagcg cgatcgcggc cgcggcagcc cagcggcgat cgatcaactc cccgtcgcga
     2161 tcgccggcga tccagatagc cacgatcgtc agcagcagaa aaaagccggc gtggcgcagg
     2221 ccgcccaacc agacatagga gtagaggatc gccagcacgg acagtggcag ccagaggaag
     2281 agaaccgcct cgcgggatcg gcgtatcgcg aatgtgatta ggagcaggac cgccacgccg
     2341 atcaggaaac cgacgctggt cggaatcgtc ggcagaaacg cgttgctgac cgtccaggca
     2401 aacgcgtcgc gattgaaaac gcgcatgacc gcctgccggg ccggatcggg cggcgtgcgc
     2461 acctggaacc aggcgagcag cgcgctcgcg gccatgagcg ccgcggcaat cgcgtcgcga
     2521 gcgccaatct gtctcctctc gatccgttcg atcacgaaga gagcggcgaa cgtcccggcg
     2581 atgaagaagc cctgcgcgtt gcagttgaaa agcagcagaa gcaccagcgc gaatgcgatc
     2641 ggccgctccc ggctgcgccg gtaaagcgct gccgccgcaa acgtgagcat tatcgtcagc
     2701 gcatagctgc gaacgacgac cgaatactcg tacgcgatgt aatacgaaaa ggtcagcagg
     2761 gttgccgtaa gccgtgacag cggcgatcgc gtcgccagca gccacacccc agccgcggca
     2821 atcgccaggt gcagcacttt ctggctggcg taggcgagac cgagcttcgc cagcggcgcg
     2881 aggcagagga accagagcgc gggcaatccc gcgtaacgcg tcctcgcaag gatggtcatc
     2941 agaccggcat cgcgtacgta cagccacgga tccgcctcgt cgcgccacgg ctcgtgatga
     3001 aaggccacga acgagacgac cgcgacgaac gcagccagca gcaaaagcgg aaaaagccgc
     3061 gacgccgagc gcgccgtgac cagcgtccgc ggatcccagt tgccgttcat cgctccggct
     3121 tccgagcgta cacctcgatc gtcccgccgc gccgcagcag cgtctccagc atcatcagca
     3181 caaacgccac cggcagcaga aagatggcgc cgattgtcgt tgcaatcgtt tgcaacggga
     3241 actcgcggat ccggtgcgcc cgcgccgccg gctctttcag aatcgaatag agcagatcgt
     3301 gctcgaagac gcggttcagg aggctctgaa tccagccgta cggattctgt tcgaggctga
     3361 agtgcgcgac ggacgtcatc cggaagccac gccggcgtag cgcatcagtc aaaccgcgcg
     3421 tcgagaagtg gacgtagtgc cgcggcacgt cgagatgaaa ccactgccgc cccgtccatg
     3481 ccgcctggat gctgacgaag ttgggaacgg cgatgacgag cagcccgccc ggctcgagaa
     3541 tctcccacgc ccgatccaac gcctgcagtg ggtcgatcat gtgctcgagg gagtgccaga
     3601 agacgactgc cttgaattcg tccgattcga aggtctggct gagaaaatcg cccgtccgca
     3661 catcgagctc gaggacgtcg cgcgcatgcg aggcggctgt cggcgaaagc tcgacgccca
     3721 caggctgata ccccgcctct cggagaaagt gcaacgtgaa cccgcggccg catccgacgt
     3781 cgagcaccgc tcctggccgg gtcagccggc ggatgacgtc gactctgcgc cgccggaacc
     3841 agcgcgtcag tctctcgaaa agcggattga accggacgtt gcgctcgccg tagtaggcct
     3901 tcggatacca cgatccgatt gccgtctcgt cgagcgccgg ccaggtacgc gccgatccac
     3961 agcgatcgca ccccagaatg gcgaagccgt cgacgacgga gtcgaaccgg tgccgcaccg
     4021 gcgacccgca tgcgagacag aatgctgtat cgggcgccgc cacggtcgcg ctcaggcgtc
     4081 gcggtgcacg ctgtccatcg agaacgccgg caggcacacc gcgatgtact ccgccccctc
     4141 ggcgcccgga gtcgagtact gcacccactc cccgctgcgc gtgatgatgg cctcgccggc
     4201 gccgacgtcg atcacgcctt cgcgcgtcgt cactcgtaac gatccacgca gaacgaccgt
     4261 gtactcctcg aactccggcg tctggcccgg ctcgacccac ccgctggggc tctgcatgcg
     4321 cgccacactg attccggcgc tcttcgaatt gacgcggccg atgtactcct cgatccgttt
     4381 cggtttgtta cccgccgctt cgatgacgga aggctgtttg atgtgcgttg gcatcgggga
     4441 agtttacctg ctccggttct cccccttcgc gttcttcgcg gcttcgcgtg ctcccggttt
     4501 tcacgcgaag acgctcacgc gaagacgcga aggcgcgaag aacagcaacg gagctctcac
     4561 ttcctcagga tgtcctcgat cgccgccagc gcgcgcgggt cgtgcttctg tcccagccag
     4621 aaggcggcct tcttccgcac gctcggatcg cgatgcgact tcatcagctc gatgagcatc
     4681 ggaaccgatt cgtcgtccgg aagctgcgac acggcaaaca ccgccttatc gcgcaccgct
     4741 gagtcgggat catcgtccac cgacgcgcga atggcgctgg cggctttgtt tcc
//
LOCUS       DATLVW010000165         3287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_584611, whole genome shotgun sequence.
ACCESSION   DATLVW010000165 DATLVW010000000
VERSION     DATLVW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584611"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VNN25_05410"
     CDS             <1..290
                     /locus_tag="VNN25_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH90996.1"
                     /translation="DERDSTQVKRATTPDKHDSTRVKRDDMASERVSTPDERDFSAGK
                     RDTTPLKPDGTANKRDGTPIKRVSTPDEGDFHPVKCDSTPVKRVFLTLAAN"
     gene            422..3151
                     /locus_tag="VNN25_05415"
     CDS             422..3151
                     /locus_tag="VNN25_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH90997.1"
                     /translation="MLQPDAMLGPYRIVSLLGEGGMGAVYRARDTRLGRDVAVKILTA
                     LTLSDQERVQRFEQEARATGMLNHPNLLTIYDVGKENGTAYIVSELLEGETLRERLER
                     GPLATRRAVDAALQVANGLAAAHEKGIIHRDLKPDNIFLTRDGRAKILDFGIAKLSAT
                     SDDGGLFPQAATEPGMVLGTVGYMSPEQVRGEKVDTRSDIFAFGAILYEMLTGVRAFK
                     RDSSIETLSAILKEDPPDLAESLPNVPPALDRLTRRCLEKDRDHRFQSARDLAFNLET
                     MAAASAAGTYSSVSNPAMGTMSGASTAQTPTVRVGDPVEPRRTSATAIRPATAAMPAT
                     ARMPVRHATVAQPLPRRVSPVLLTLLFLAAIGGAVFAGWYLATNKMKEEAPPEVVFHR
                     MTFRRGEVRAARFGPDGDSIVYSAAWDGNPSEVFVANRQSPEPRPLGVKDADVLAVSK
                     STELAVLLRRDRLTSLGTLARVPVAGGTPREIAEQVLQADWSPDGTNLVAIRGGNGTF
                     RIESPIGKVLYETPHALRDIRVASDGRIAFVESYSGKSDVSILGAKSGKPVSIARGWS
                     HGVTGLAWAPGNKEIWITATDTGAPPALYAVNTETGESRLVNRLTGSMRLYDIAPAGH
                     VLLSNGMWRAALEVQAPGESVERDLSWLDWSMLADLSRDGRTILFNEPREGGGAASSM
                     YLRRLGEPTPVRIADGYGDAISPDGKYVLGHSGPKLVVVPTGSGESRELKIEGAFDFG
                     AAWLPDGRRIVIAGALPKKGYQLHVIDTLDETEKAISPENIWGDATRPFAVSPDGRYV
                     AGMTQQQTIALYPVDASAEPSIVPAIQPGEVPIVWSADGASLFVYHPSALPAQVTRVT
                     LATGAREAWKQFSPTEPAGVYRIAPICITPDATAYAYDALRMLGDLYVVDGLR"
BASE COUNT          608 a         1142 c         1017 g          520 t
ORIGIN      
        1 cggatgaacg cgacagcacg caggtaaaac gcgccaccac gccggataaa cacgacagca
       61 cgcgggtaaa acgcgacgac atggcgagtg aacgcgtcag cacgcccgat gaacgcgatt
      121 tttccgccgg gaaacgcgac accacgccgt taaaacccga cggcacggcg aataaacgcg
      181 acggcacgcc gataaaacgc gtcagcacgc ccgatgaagg cgattttcac ccggtaaaat
      241 gcgacagcac gccggtcaaa cgtgtatttc tcacactcgc agcgaattga agcagtgagt
      301 acccatcgag gagcgcaaag tcagcactct cagagctcga cggcaccgcc cattgatact
      361 cattcccact ccccaccccc gacacgccac cccccttccg ctgctaccat gcgctcatcc
      421 catgctgcag cctgacgcga tgctcggtcc ctatcggatc gtcagccttc tcggcgaagg
      481 agggatgggc gctgtctatc gcgcccgcga cacccgcctc gggcgcgacg tcgcggtcaa
      541 gatcctcacc gcgctcacac tcagcgacca ggagcgcgta cagcgcttcg agcaggaagc
      601 acgcgccacc gggatgctca accatcccaa cctgctgacg atctacgacg tcggcaaaga
      661 gaacggcacg gcttacatcg tcagcgagct gctcgaagga gagacccttc gcgagcgatt
      721 ggagcgcggg ccgttggcga cgcggcgcgc ggttgatgcc gcgctgcaag tcgcgaacgg
      781 cctcgccgcg gcgcacgaga aaggcatcat ccatcgcgac ctgaagcccg acaacatctt
      841 cctcacgcgc gacggccggg ccaagatcct cgacttcggc atcgccaaac tgagcgccac
      901 ctcggacgat ggcggcctct tcccccaggc cgccaccgag cccggcatgg tcctcggcac
      961 ggtcggctac atgtcgccgg aacaggtgcg cggcgagaag gtcgacacgc ggtccgacat
     1021 cttcgcgttc ggcgcgatcc tctacgagat gctgacgggc gtacgcgcgt tcaagcgcga
     1081 ctcgtcgatc gagacgctca gcgcaatcct gaaagaggat ccgcccgacc tcgccgagtc
     1141 gctgccgaac gttcctccgg cactcgaccg cctcacgcgc cgctgcctcg aaaaggaccg
     1201 agaccatcgt ttccagtctg cccgcgatct cgccttcaat ctcgaaacga tggcggcggc
     1261 ttcggcggcc ggaacctatt cgagcgtctc caatcctgcg atgggaacaa tgtcgggagc
     1321 atccacagcg cagacaccga ccgtgcgtgt cggcgacccc gtggaaccac gccgcacctc
     1381 cgcaaccgcg atccgccctg ccaccgctgc gatgccggcg acggcgcgca tgcctgtgcg
     1441 ccacgcgacc gtcgcacaac cgttgccgcg gcgcgtgtct ccggttcttc tgacgcttct
     1501 ctttcttgct gcgatcggcg gcgccgtgtt tgccggctgg tacctcgcga caaacaagat
     1561 gaaggaggaa gcgcctcccg aggtcgtctt tcaccggatg acgttccggc gtggggaagt
     1621 acgcgcggcg cgcttcggtc cggacggcga ttcgatcgtc tacagcgcgg cgtgggacgg
     1681 caatccgtcc gaagtcttcg tcgccaaccg gcagagcccg gagccgcggc cgctcggcgt
     1741 gaaagacgcc gacgtccttg ccgtctcgaa atcgacagag ctggccgtcc ttcttcgccg
     1801 cgaccgtctc acaagcctcg gcacgctggc acgcgttccg gtcgcggggg gaacaccgcg
     1861 cgagatcgcc gaacaagttc tgcaggccga ctggtcgccc gacggcacga acctcgtcgc
     1921 catccgcggc ggcaacggca cattccgcat cgagtcgccg atcggcaagg tcctttacga
     1981 aacgccacac gcgttgcgcg acatccgcgt cgcatccgat gggcgtatcg ccttcgtcga
     2041 gtcgtactcg gggaagagcg atgtctcgat cctcggcgca aaatcaggca agccggtttc
     2101 gattgcgcgc ggctggagtc acggagtcac cggtctggcc tgggctccgg gcaacaagga
     2161 aatctggatc accgcaaccg acaccggcgc gccgccggcg ctttacgcgg tcaacaccga
     2221 gactggcgag tcgcggctcg tcaaccggct gaccggatcg atgcggctgt acgacatcgc
     2281 ccctgccggg cacgtgctac tgtcgaacgg gatgtggcgc gcggcgctgg aagtacaggc
     2341 accgggcgaa tcggtggagc gcgatttgtc gtggctggac tggtcgatgc tggccgatct
     2401 ttcgcgcgac ggaagaacga tcctgttcaa tgaaccgcgc gaaggcggcg gcgcggccag
     2461 ctcgatgtac cttcgccgcc tcggcgagcc gactccggtc cggatcgccg acggatacgg
     2521 cgacgccatc tcccccgacg gcaagtacgt tctcggtcac agcgggccga agctcgtcgt
     2581 ggtcccgacc ggaagcggcg agtcgcgcga gctcaagatc gaaggtgcgt tcgacttcgg
     2641 cgccgcctgg ctccccgacg gccgccgcat cgtcatcgct ggcgcgttgc ccaagaaggg
     2701 ctatcagctt cacgtcatcg acacgctcga cgagacggag aaggcgatct cgccggagaa
     2761 catctggggc gatgccaccc gtccgtttgc cgtctcgccg gacggccgct acgtcgccgg
     2821 aatgacgcaa cagcagacga tcgcgctcta tcccgtcgac gccagcgcgg agccgtcgat
     2881 cgtccccgcg atccagccgg gggaagtgcc catagtctgg tcggccgacg gcgcatctct
     2941 ctttgtgtat caccccagtg cccttcccgc ccaggtgacg cgggtgactt tggccaccgg
     3001 cgcgcgggaa gcgtggaagc agttctcgcc caccgagccg gccggcgtgt acagaattgc
     3061 gccgatctgc atcacgcccg acgccaccgc ctacgcctac gacgccctgc ggatgctcgg
     3121 cgatctgtac gtcgttgacg gactgcggta accgcgcggt gaagcaacgc gttagagcga
     3181 tcagcgttca cggcgcgcga tcagccgccg atcaggacgt tttcgcggcg cggtgcggtt
     3241 ctcacgagtc acttggcgaa cttggctttg gcatcccggc aagctat
//
LOCUS       DATLVW010000166         2858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_88566, whole genome shotgun sequence.
ACCESSION   DATLVW010000166 DATLVW010000000
VERSION     DATLVW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88566"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VNN25_05420"
     CDS             <1..364
                     /locus_tag="VNN25_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH90998.1"
                     /translation="TAGTNFRAWIYRILRNTFLTSKSGLRGAPQVSIDDDPDRFEGLA
                     EEATPETIFFSRANAAALQTAIEALPLPFREVILLSDVENFSYKEIAETLSIPIGTVT
                     SRLLRARAKVREALRGSK"
     gene            364..1038
                     /locus_tag="VNN25_05425"
     CDS             364..1038
                     /locus_tag="VNN25_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXH90999.1"
                     /translation="MQDLTAEEVERYADGEIPPEALPEIEAHLHDCSRCANAVLGVMQ
                     MKRAVRAAMPRFTPRRPLVLRKRAPRTFVWFAAAAALIVIAIGGEMLVLSRNTSAREL
                     ADLHSTILASANPIDVISTDRHTVKPWFEGRVPFAVNVPDLSATPFRLIGGRVVFWHG
                     RPGAYLLVSKGAHKISLFIFESLPSLGTTPQMTIDEWRTNDLTYVAVGDVSRDDLEAL
                     RRAFGG"
     gene            1132..1365
                     /locus_tag="VNN25_05430"
     CDS             1132..1365
                     /locus_tag="VNN25_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH91000.1"
                     /translation="MKHKTASLFWTGRSQAVRLPKEFRFEGEVVLVRRDGKAVILEPA
                     IGWPQGYVDSFKGIPDDFERPPQGKIEKRQKLG"
     gene            1373..1771
                     /locus_tag="VNN25_05435"
     CDS             1373..1771
                     /locus_tag="VNN25_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH91001.1"
                     /translation="MPFLLDTDTCIYALKQNPAVLDQLLSKSREEIVVSVITEAELRT
                     GAAKSSSPVRTLRLVENFLGPLALAEFTSDDAIAYAHIRAKLERAGTPIGPLDTLIAA
                     QAIARKLTLVSNNEREFRRVPRLQLENWTR"
     gene            1892..>2858
                     /locus_tag="VNN25_05440"
     CDS             1892..>2858
                     /locus_tag="VNN25_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXH91002.1"
                     /translation="MPTRETDDTRVTPDDPRYLAVIDKRFNKRFKASPDYVRIVRSTG
                     AAVAAVDEAVREGRRLVVTSGGHCLEGFVSDADVRVIVDMAPMKTVYYDAEMRAVAVE
                     AGATVGEAYRALFETWGVVVPLGEYPGIGMGGHVAGGAFGFLCRQLGLAADYLYGVET
                     VTVDESGIALSVVATRETSDPNRELWWAHTGGGAGNFGIVTRYWFRSPDADGDDPAAL
                     LPRAPESITTFKAEWNWSDIDEQSFLRLVRNHGIWCEQNSGADSPNASLFTLLEVHRK
                     QFGKIIARGVSTAGVSAKRQVDEHLTALSEGIVAPGGREVTRMSWL"
BASE COUNT          533 a          863 c          937 g          525 t
ORIGIN      
        1 caccgccgga acgaactttc gcgcctggat ctaccgcatc ctgcgcaaca cattcctgac
       61 gtcgaagagc ggcctgcgag gcgcgccgca ggtttcgatc gacgacgatc ccgatcggtt
      121 cgagggattg gcggaggaag cgacgccgga gacgatcttc ttcagtcgcg ccaacgccgc
      181 ggcgttgcag acggcgatcg aagcgctacc gctgccgttc cgcgaagtca tccttttgtc
      241 ggacgtcgaa aacttcagct acaaggaaat cgcggagacg ttgtcgattc cgatcgggac
      301 ggtgacgtcg cgtctcctgc gcgcgcgggc caaagtgcgc gaagcgttgc gaggatcgaa
      361 atgatgcaag acctgactgc ggaggaagtg gagcggtacg cggacggaga gattccgccc
      421 gaggcgctgc cggagatcga ggcgcatctc cacgattgca gccgttgcgc gaacgcggtg
      481 ctgggggtga tgcagatgaa gcgcgcggtc cgcgccgcga tgccccgctt tacgccgcgc
      541 cgtccgctgg tgctgcgaaa acgcgcgccg cgaacgttcg tatggtttgc cgcagcggcc
      601 gcgctgatcg tcatcgccat cggcggcgag atgctcgttc tctcccgcaa cacctcggcg
      661 cgcgagctgg ccgatctcca ttcgacgatc ctggccagcg cgaatccgat cgatgtcatc
      721 tccaccgacc gccacaccgt gaagccgtgg ttcgagggtc gggtgccatt tgcagtcaac
      781 gttcccgatc tctcggccac gccgtttcgc ctgatcggtg gccgtgtcgt cttctggcac
      841 ggccgtccgg gcgcctacct gctggtcagc aaaggcgcgc acaagatctc gctcttcatt
      901 ttcgagtcgc tccccagcct cggcaccacg ccgcagatga cgatcgacga atggcgcacg
      961 aacgacctga catacgtggc agtcggcgac gtttcccggg acgacctcga ggcgctacgt
     1021 cgggcgttcg gagggtaggg ggcggtaggg gggcggcgat cgaagtgaag aaacggtttt
     1081 gatcggtccg ccttgacaat tcgttaccgg tacctatcat cggtagctac catgaaacac
     1141 aaaacagcca gccttttctg gaccggacga agtcaggccg tgcgcctccc gaaagagttc
     1201 cgtttcgagg gcgaagtcgt cctggtgcgc cgcgacggga aggctgtgat tctcgaaccg
     1261 gcgatcggat ggccccaggg ctatgtcgat tccttcaaag gcattcccga cgatttcgag
     1321 cgtccgccac aagggaagat tgagaagcgc cagaagctgg gatgacgaca gtatgccgtt
     1381 cctgctcgac accgacacgt gcatctacgc gctcaaacag aatcccgccg ttctggatca
     1441 gttgctttcg aagagccgcg aagagatcgt ggtcagcgtg atcaccgagg ccgagcttcg
     1501 gaccggagca gccaagagct catcgcccgt caggactctt cgcctcgtcg agaacttcct
     1561 tggcccgctc gccctcgccg aattcacctc ggacgacgcc atcgcctacg cccacatccg
     1621 cgcgaagctg gaacgcgccg gcactccgat cggtcccctc gacaccctca tcgccgctca
     1681 ggcgatcgcc cgaaagctca ccctcgtttc aaacaacgaa cgcgagtttc gaagagtgcc
     1741 gagattgcag ctcgagaatt ggacgcggtg agaaagggta agggtaggtc acgaaacagg
     1801 agagtcaggg cgcagccatc ttcttccgga tctcgttgat tgccatccgt ttgatctgag
     1861 cactagcggc aacggatatt cttgcaagac gatgccgaca cgagaaaccg acgacacccg
     1921 agtcactccc gacgatcccc gctatctcgc ggtgatcgac aagcgtttca acaaacgctt
     1981 caaagccagt ccggactacg ttcgtatcgt tcgctcgacc ggcgccgcgg tcgccgcggt
     2041 cgatgaggcg gtgagggagg ggcggcggct cgtcgtcacc agtggcgggc attgcctcga
     2101 ggggttcgtg tccgatgccg acgtgcgcgt gatcgttgat atggcgccaa tgaagacggt
     2161 gtactacgac gcggagatgc gggccgtggc ggtcgaggca ggggcaaccg tcggcgaggc
     2221 atatcgggca ttgttcgaga cttggggagt cgtcgttccg cttggcgagt atccggggat
     2281 tgggatgggc gggcatgtgg ctggcggcgc gtttgggttc ctctgccggc agcttgggct
     2341 ggccgcggat tatctctacg gcgtcgagac ggtcacggtc gacgagtccg gaatagcgtt
     2401 gagtgtggtg gcgacacggg agacgtccga tccgaatcgc gagctctggt gggcgcatac
     2461 cggcgggggc gcgggcaact tcggcatcgt cacgcggtac tggttccggt cgcctgacgc
     2521 tgacggcgac gatccggccg cgctgctgcc tcgcgcgccg gagtcgatca ctacgttcaa
     2581 agcggaatgg aactggagtg acatcgacga gcagtcgttc ctgcgactgg tcaggaatca
     2641 cgggatctgg tgcgagcaaa acagcggcgc tgattctccg aacgcctcgc tcttcacgct
     2701 cctcgaagtc catcgcaagc agttcgggaa gatcattgct cgtggtgtga gcacagccgg
     2761 cgtgtccgcc aagcgacagg ttgacgagca tctgacggcg ctcagtgagg gaatcgtcgc
     2821 gccgggtggc cgggaggtga cgcggatgtc gtggctcg
//
LOCUS       DATLVW010000167         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_295793, whole genome shotgun sequence.
ACCESSION   DATLVW010000167 DATLVW010000000
VERSION     DATLVW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295793"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VNN25_05445"
     CDS             <1..351
                     /locus_tag="VNN25_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91003.1"
                     /translation="TCANETTAASKVEAPLACNLLALTADERKRHFEVVGPALRRLRT
                     GVRELPNGYEFQFPSDAKTVALLNEWVGRERRCCPFFEMDVRTGPKGGPLSLRLTGRD
                     GTKEFIRMSEWITR"
     gene            complement(474..1382)
                     /locus_tag="VNN25_05450"
     CDS             complement(474..1382)
                     /locus_tag="VNN25_05450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH91004.1"
                     /translation="MRSNPRISIVIPSLNQGRFIEETILSILGQAWPDIELIVIDGGS
                     IDETLDVIRDYEASIDFWISEPDRGQADAINKGLRKATGEIVTWFGADDVYANGIFAS
                     VAEAWRRNPDAIYAAPVANFYARGREQLVRPFGLSVENVVQYWKRQSRWHDPGLFWSR
                     AAINAVGEIDVSLRYALDYDYLIRALQHASVAYIDQVAAGFRLHGRSKTIAQTEAMMA
                     ETASVSRRYWGLVENLDRTGFERSEYEVRLRRAASKLVRLNREGLPLLRACLREHLFA
                     TPVRLAWVFPTVLAERLGRLRRGRHI"
     gene            1527..2243
                     /locus_tag="VNN25_05455"
     CDS             1527..2243
                     /locus_tag="VNN25_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HXH91005.1"
                     /translation="MNMTLTQKQAPEGMAGISGFKLIIGIFFAILGVLLTLDNLDLID
                     ADRYLAYWPLVLVAIGILKFQEAGHRVLGAFLIGIGALNLISNTVDLRFSVFDLWPAA
                     LILAGIGIVAHAFGFRLPSLSGQSESTIWAIFGVRKIKIGSPAYTGARIIAFMGGCEL
                     DLTKADMEQGPATIELFVMWGGIEIKVPEGWEVIGNTVPIMGGAEIRTKAAPGGRRLI
                     VNGLAFMGGVEIKSVAAEAV"
     gene            2240..2611
                     /locus_tag="VNN25_05460"
     CDS             2240..2611
                     /locus_tag="VNN25_05460"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HXH91006.1"
                     /translation="MKNRRRINADGLFWGLLLIAGGSLLLLQRLHIANFSWKLGSWWP
                     LFIVLVGMSKLAHRRSIWSGLWLIAVGAWLQAVTLHWYGLTYNSSWPLLLVILGAGMI
                     GRTIIESFRRRDALEGENHHE"
BASE COUNT          513 a          845 c          857 g          547 t
ORIGIN      
        1 acgtgcgcca acgagaccac cgctgcttca aaggtcgagg cccctctcgc ctgtaacctg
       61 ctggctctga cagcagatga acggaaacgg cacttcgaag tcgtcgggcc ggctcttcgt
      121 cgattgagaa ccggcgtgcg cgagcttccg aacggttacg agtttcagtt tccgagcgat
      181 gcgaagaccg tcgcacttct caacgaatgg gtcgggcgcg aacgccgctg ctgcccattc
      241 ttcgaaatgg atgtgcggac gggtccaaaa ggcggcccgc tgtcgcttcg tttgaccgga
      301 agagatggga cgaaagagtt cattcgcatg agcgagtgga tcacgcgatg agttaggcga
      361 ctgaaggagt gcggtcgtct cgaccgcgga cactcgggcg tcctcgcccg agtgtcgtca
      421 gggcggcggt cgcaattcgt cggcgctctt ggtagctttg ccgtgcagaa cccctagatg
      481 tgcctcccgc ggcgcaaccg gccgagacgc tccgcgagca ctgtcggaaa cacccacgcc
      541 aatcggaccg gcgttgcaaa tagatgctcg cgcagacacg cacgcaagag gggcaggcct
      601 tcgcggttga gccgcacaag cttcgaggcc gcccgccgaa gtcgcacctc gtactccgag
      661 cgctcgaagc cggtacgatc gaggttttca accaaccccc aataacgccg gcttactgat
      721 gccgtctcgg ccatcatcgc ctccgtctgc gcgatcgtct tcgaccgccc gtgcagccga
      781 aagccggcgg caacttgatc gatgtacgcc acggacgcgt gttgcagcgc acggatcagg
      841 tagtcgtagt ctaacgcata gcgcagagaa acatcgatct cgccgacagc attgatcgcg
      901 gcacgcgacc aaaacaaacc cggatcgtgc cagcgtgact gcctcttcca gtactgcacc
      961 acgttctcga ccgagaggcc gaacggacga acgagctgct cgcgtccacg cgcgtaaaaa
     1021 ttcgcaacag gggccgcgta gatggcgtcg ggattccgcc gccaagcctc cgcgacggac
     1081 gcgaagatcc cattcgcgta gacatcgtcg gcgccgaacc aggtgacgat ctcgccggtc
     1141 gctttgcgaa gacctttgtt gatcgcgtcg gcctgtccgc gatcgggctc gctgatccag
     1201 aaatcgatcg atgcctcgta atcgcggatg acgtccagcg tttcgtcgat cgacccacca
     1261 tcgatgacga tgagctcgat gtccggccac gcctgcccga ggatcgagag gatcgtctct
     1321 tcgatgaacc ggccctggtt cagcgacggg atgacgatcg agatgcgcgg attggatctc
     1381 atcgccgcga gagtaccgcg gccgttcagc gcggaaaacg cccgagtgcg ccattcgccg
     1441 ctttttagcc gccattcgcc ggtggagcgc agacgcagcc ggccaggctt cgtatcttgc
     1501 ggctgtcgct gacgaaagga tttctcatga acatgacact cacacagaaa caggctcccg
     1561 aggggatggc tggcatctcc ggcttcaagc tcatcatcgg aatcttcttc gcgatcctcg
     1621 gcgtgctgct gactctcgac aacctcgatc tgatcgatgc ggaccgctac cttgcctact
     1681 ggccgctcgt cctggtcgcc atcggtatcc tcaagttcca ggaggccggg cacagggttc
     1741 tcggtgcgtt tctgatcggc atcggcgcgc tcaacctgat ctccaacacg gtagatcttc
     1801 gcttttcagt cttcgacttg tggccggccg cgctgatcct ggccggaatc gggatcgtcg
     1861 ctcatgcctt cggttttcgc ctgccgtcgc tttccggcca gtccgaatca accatttggg
     1921 caattttcgg cgtccgcaag atcaagatcg gctcgcccgc ctacaccggc gcccggatca
     1981 ttgccttcat gggcggatgt gagctcgacc tgacgaaggc cgacatggag cagggaccgg
     2041 cgacgatcga gctgttcgtg atgtggggcg gcatcgagat caaggtgccc gagggatggg
     2101 aagtcatcgg caacaccgtt ccgatcatgg gcggtgcgga gatccgaacg aaagcagcac
     2161 cgggaggccg caggctcatc gtcaacggac tggcgttcat gggcggcgtc gagatcaaga
     2221 gcgtcgcggc ggaggcggta tgaagaatcg acgacggatc aatgccgacg gccttttctg
     2281 gggcttgctt ctgatcgcgg gcggatctct gctgcttctt cagcgtcttc acatcgctaa
     2341 tttttcgtgg aaacttggat cgtggtggcc gctcttcatc gtgttggtcg ggatgtcgaa
     2401 gctcgcccat cgacgctcga tctggtcggg gttgtggttg attgcagtgg gtgcgtggct
     2461 gcaggcagtg acgcttcatt ggtacggcct gacttacaac tcgtcgtggc ctttactact
     2521 cgtcatccta ggcgccggga tgatcgggcg cacgatcatc gagtcgttcc ggcgccgcga
     2581 cgcgctcgaa ggcgagaacc atcatgaatg acgacgaacg gcggcgcagg tcgatcgacg
     2641 ccgatggcct ctcgcgcatc ggccttcgca tcaaatcccg taagcgcggc gataatccgt
     2701 gggcgcggct ggtgtggggc gcggcgattc tcactgcggg cgtgattggc tggctcgatc
     2761 ac
//
LOCUS       DATLVW010000168         4450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_378063, whole genome shotgun sequence.
ACCESSION   DATLVW010000168 DATLVW010000000
VERSION     DATLVW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378063"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..1782
                     /locus_tag="VNN25_05465"
     CDS             553..1782
                     /locus_tag="VNN25_05465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXH91007.1"
                     /translation="MAARALTAVIAFALFTALPAAATPASIDAGATILCYHIVESPHD
                     PRMEVSREAFHQQMRYLAMTGYNVIPLRQLYDYVTGKRASLPKNAVVITFDDGWRSTY
                     TEVFPEMKRYGFPFTIFIYPAIVGQTSHAINWKQLKEMSDAGVDIESHSLSHPFLTRR
                     RHADLDQKAYNAWLEKELSDSKRLLERATSKPVQFLAYPFGDYDHFLVDSVSKAGYEA
                     ALTCESGRVRRGSNPLRMRRVVVEKRVDFVSFRRYLGAGSMRVDEMTPLPGVVYDPTP
                     MVTISAKIPHHESIDPKSVGMALLSAAGSLPYSYDPETGAISVAAVKESLQGTYQRAL
                     VWATDAKSGKRVEASWVFKLPEPRLPIVPSLVSDPQRVTPGPETAPQGQSGAAQAIVS
                     SGGGSGSITRPQRAPKG"
     gene            complement(1923..2654)
                     /locus_tag="VNN25_05470"
     CDS             complement(1923..2654)
                     /locus_tag="VNN25_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXH91008.1"
                     /translation="MKKLVALFLFAAAATAVHAQDADLINPDRPGLADGSGVVGRGVF
                     QLEAGLERDHASDGVSISTPLLLRYGLTKAVELRVEGNGLVHSSGTNEIAPLSIGAKV
                     HFADAPSLGVIVRAFVPSGSGSERSHSTTGDVRLAADINLGERWSLNPNIGVASLDDG
                     DGRFTAGLAALTVQYNLSDHANVFVDGALQSPESHGGSTALTVDAGAVRIIGSNTQLD
                     FSIGWGARGTTPPNVFWSAGISRRF"
     gene            2868..3164
                     /gene="yhbY"
                     /locus_tag="VNN25_05475"
     CDS             2868..3164
                     /gene="yhbY"
                     /locus_tag="VNN25_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HXH91009.1"
                     /translation="MALTSKQRQYLRGLAHALEPVVRVGRGGVSQSVIEETKRSLAAH
                     ELIKVRIENDDSAQRRTLGEELARACDAEVAGSIGKLAILYRAKDEEPEIELPA"
     gene            complement(3264..>4450)
                     /locus_tag="VNN25_05480"
     CDS             complement(3264..>4450)
                     /locus_tag="VNN25_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXH91010.1"
                     /translation="MGYDLYRLNASIGYGLTDNWEISASLPYDRITGHGGDRNGFING
                     FYYQGKFSDAGMAPLHLATKFSFGPSSATSHLAVSLFADLPTGNKDSGIASGSTNFGA
                     GLHYTASQFTVGGSYTIVGDRSAKNSNFPFLAPDSEKLPNELLLEVGWNHPIHFWRTT
                     NWITEISTTTYTGGDVKPPSPVTLVTGLRHWIGDSGWAINGGVRWNAAKFSRDHKECR
                     VTELDDCALSGLVGITFAPLHLAAVAPPPPPPAPLPEPPPPPPPPTPVAPPEPPPVTV
                     PHVPTELRTDEIHFEAGSARLSNIAKAILDDVALRMKQEPTATAIAIGYNDDKENTGP
                     NKDLDRRRAEAVRDYLVTRHGIDPARITIEGRDGREPVGDNTTGEGRLRNRRVVIRLM
                     LP"
BASE COUNT          837 a         1377 c         1431 g          805 t
ORIGIN      
        1 ggttgtgggg agtgggttgt ggggagtggg ttgtggggag tggggagtgg ggcgtgggag
       61 caggaccgac gcatggagca ggaccgacgc atgtgctcct ccttgcgatg cctcttatcc
      121 ttcttccttg gagtgcttcc ccatcgagcg ccttctcccc ccgcgctttt tgcgggggga
      181 gaaggtggcg aagccggatg aggggggcgc aaatacgcag ggcgatggat cttgccttgt
      241 ccgaagtgtc tgaaaacggg cgacatgttg ggatgtcgta cgcattggaa cgccccctca
      301 tccggccttc ggccaccttc tcccccacaa aaagcgtggg ggagaaggcg ctcgattgga
      361 aggtttgccg caagagtgtg cgaaaagccg cccacccgac cacgcggcaa cccagaaacc
      421 cgagaaacca cctcgtgatc gcgcacacat ctcctcgtga tcgcgataca ataaccactg
      481 cgctcgcagg taccgttgtt gcagaggggc gaacgcaatc atctccttta ccatcaatca
      541 ctcggagctc ccatggccgc gcgggcgttg acagcagtca ttgcgtttgc acttttcact
      601 gctctacctg cggctgccac gcctgcgagc atcgacgcag gcgcgaccat cctctgctac
      661 cacatcgtcg agtcccctca tgacccgcgc atggaggttt cgcgagaggc gttccaccag
      721 cagatgcgtt acctggcgat gaccggctac aacgtcattc ctcttcgcca gctctatgac
      781 tacgtgaccg gaaagcgggc ctcgcttccg aaaaacgcgg tcgtcatcac cttcgacgac
      841 ggatggcgca gcacctatac cgaagtcttc ccggagatga agcgctacgg atttccgttc
      901 accatcttca tttatccggc gatcgtcggc cagacttcgc acgcgatcaa ctggaagcag
      961 ctcaaagaga tgtccgatgc cggcgtcgac atcgagagcc actctctttc gcatccgttt
     1021 ctcacacgcc gccgtcacgc cgatctcgat caaaaggcct acaacgcctg gctcgaaaaa
     1081 gagttgagcg acagcaagcg tctgctcgag cgcgccacat cgaagccggt gcagttcctc
     1141 gcctatccgt tcggcgacta cgatcacttc cttgtcgaca gcgtcagcaa agcgggctac
     1201 gaggctgcgc tgacgtgtga gtcgggacgc gttcgccgcg gcagcaatcc gctgagaatg
     1261 cgccgcgtcg tcgtcgagaa gagagttgat tttgtcagct tccgccgtta cctcggcgcc
     1321 ggctcgatgc gtgtcgacga gatgactccg ctgcccggcg tggtttacga cccgacgccg
     1381 atggtcacca tctccgccaa gatcccgcat cacgagtcga tcgatccgaa gtcggtcggg
     1441 atggcgctcc tctccgccgc aggctcgctc ccctactcgt acgaccccga gaccggcgcg
     1501 atctccgtcg ccgcggtcaa ggaatcgctg cagggaacct atcagcgtgc cctggtctgg
     1561 gccaccgacg ccaaatccgg caagcgcgtg gaggcgagct gggtgttcaa gctgccggaa
     1621 ccgcggttgc cgattgtgcc gtctctcgtg agcgacccgc aacgcgtcac tcccggcccc
     1681 gagaccgcgc cgcaagggca atccggcgca gcgcaggcaa tcgtctccag cggcggaggc
     1741 tcgggatcga tcacgcggcc acagcgcgcg ccgaagggat agaaaagaga tcagagacaa
     1801 aagagattag agacaaaggg tggacttggg tcctatctct gatctcttgt ctctgatctc
     1861 tgtctctacg gagtttttac gtctccgcgt ctccgcgtga ccaatcctcc tgctccggat
     1921 agctagaacc gccggctgat ccccgccgac cagaacacgt tcggcggtgt cgtccctcgg
     1981 gcaccccaac cgatggagaa atcgagctgc gtgttgctcc cgatgatcct gacagcgccg
     2041 gcgtccaccg tcagcgccgt cgagccgccg tggctttccg gagactgcag cgcgccgtcg
     2101 acgaagacat tggcgtgatc cgagaggttg tactgcacag tgagcgcggc cagtccggcg
     2161 gtgaaccgcc catcgccgtc gtcgagcgag gctacgccga tgttgggatt gagcgaccat
     2221 cgctcgccga ggttgatgtc ggcggccaga cggacatcgc cggtcgtcga gtgagagcgc
     2281 tcggaaccgc tgcccgaagg aacgaaggcc cgtacgatca caccgagtga aggcgcgtca
     2341 gcaaaatgaa ccttcgcgcc aatcgacagc ggcgcgatct cgttggttcc gctggaatga
     2401 actaggccgt ttccttcgac gcgcagctcg accgcttttg tcagaccgta gcgcagcagc
     2461 aaaggcgtcg agatcgatac gccatccgag gcatgatcgc gttcgaggcc ggcctcaagc
     2521 tgaaagactc cacgcccaac gacaccgctg ccgtcagcga ggccaggccg gtccggattg
     2581 atgagatccg cgtcctgagc atgaacggca gttgctgccg cagcaaagag aaagagcgca
     2641 acaagtttct tcatacgcga agagtatcaa ggagtgcagg cgtctcgcct gcggccgttg
     2701 ggcgtcccgc ccaacggtgg tgtccgaaat cgacaaacgt cactcgacgg attccagcaa
     2761 tggccgccat caaaacacgg actctcgtcg tcgccgcgca attccgtcgg gcgagacgcc
     2821 cgccggccgc aggcgagacg cctgcactcc tgttaccatg cccgcaaatg gcgctcacat
     2881 cgaaacagcg gcagtacctc agggggctgg cccatgctct cgagccggtc gtgcgcgtcg
     2941 ggcgtggcgg cgtcagtcag tcggtcatcg aagagacgaa gcgctcgctg gccgcccacg
     3001 agctgatcaa ggtccgcatc gaaaacgacg attccgcgca gcgccgcacg ctcggcgagg
     3061 agctggcgcg cgcgtgtgat gccgaggtgg cgggaagcat cgggaagctg gcgattctct
     3121 atcgggcgaa agacgaagag cccgagatcg aattgccggc gtagcggcgc cgccagtctg
     3181 gcgggaacgc aatcaaacaa cgctccacaa aaacgaaggg cagcctcgcg gctgcccttc
     3241 gcaggtaact gctgtgaaga cgattacgga agcatcaggc gaatcactac acggcgattg
     3301 cggaggcggc cttcgccggt ggtgttgtcg ccgaccggct cgcggccgtc gcggccttcg
     3361 atcgtgatgc gcgcgggatc gattccgtgg cgcgtcacga ggtagtcgcg aaccgcttcg
     3421 gcgcggcggc gatcgaggtc cttattcgga cccgtgtttt ccttgtcgtc gttgtagccg
     3481 atggcgattg cggtcgcggt gggctcctgc ttcatgcgca gagcgacgtc atcgaggatg
     3541 gctttggcga tgttcgacag acgcgccgaa ccggcttcga agtggatctc gtcggtgcgg
     3601 agctcggtcg gaacgtgcgg gacggtcacc ggaggcggct ccggcggagc gaccggtgtc
     3661 gggggaggcg gcgggggcgg gggctccggc agcggggcgg gcggcggagg cggcggcgcg
     3721 acggctgcaa gatgcagcgg tgcgaacgtg attccgacca ggcccgacag tgcgcagtcg
     3781 tcgagctcgg tgacgcggca ttccttgtga tcgcggctga acttcgctgc gttccagcgg
     3841 acgccgccgt tgatggccca accgctgtcg ccaatccagt ggcgcagacc ggtcacgagc
     3901 gttacgggcg acgggggctt cacatccccg cccgtataag tcgtggtgct gatttccgtg
     3961 atccagttcg tggtgcgcca gaagtgaatt ggatgattcc agcccacttc gaggagcagc
     4021 tcattcggca gcttctccga atcgggagcg aggaacggga agttcgaatt cttggcgctg
     4081 cggtcgccca cgatcgtgta gctaccgccc accgtgaact ggctggccgt gtagtgcaga
     4141 ccagcgccga agttcgtgct tccgctggcg atgccgctgt ccttgttgcc ggtcggaaga
     4201 tcggcgaaca gcgagacggc cagatgcgat gtggctgacg acggaccaaa actgaacttc
     4261 gtggccaggt gcagtggcgc catgccggcg tcggagaact tgccctggta gtagaagccg
     4321 ttgatgaaac cgttgcggtc tccgccgtga ccggtgatgc ggtcataagg cagcgacgcg
     4381 ctgatctccc agttgtccgt gaggccgtag ccgatcgagg cgttcaaccg atagaggtca
     4441 tagcccatgt
//
LOCUS       DATLVW010000169         7863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_88702, whole genome shotgun sequence.
ACCESSION   DATLVW010000169 DATLVW010000000
VERSION     DATLVW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88702"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VNN25_05485"
     CDS             <1..919
                     /locus_tag="VNN25_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXH91011.1"
                     /translation="YGNGEDNIVTAVGTFSMSTITASSSVAASSTGNTASVTPSNGTT
                     VTYAWTITGGTITSATNIASITYTAGASGSVVLRATAYNTRANNGSGFPSGTVGVTDT
                     KTVTIVTVSPPTNVVAAALTSTSVQVTWTAASGATKYEISRSANNSTFTAVCVVASTC
                     PTSSPFTDSTASANTAYIYKVKSVDAGSNLSAFSNGDLATTVIFTDSLVQNTTKVKLV
                     HMNELRTAMTAVCTLAANPSPCSTAFTDPTLSTSTKVSVLHLTELRAKLNAARSTLGL
                     TAQSYADGSITTATPIRAAQINELRNGVQ"
     gene            complement(930..1490)
                     /locus_tag="VNN25_05490"
     CDS             complement(930..1490)
                     /locus_tag="VNN25_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXH91012.1"
                     /translation="MRRGILFVPLLLAGCGFLSSKPSTFYSLDRIPSQAPVAVVRGLP
                     IGIDVVLPPGLNRKELVVRQANHQLEVRGREQWTTTLQPLVLHTLAFDLASRLPEGMV
                     ILPGQSQPDGGMRSLDVVVEELAAGPDHAVVLDARWTMHESGRADITRREHIVTDIPS
                     LDSANVATGISNAIGTLADHIVGQVR"
     gene            complement(1493..3253)
                     /locus_tag="VNN25_05495"
     CDS             complement(1493..3253)
                     /locus_tag="VNN25_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXH91013.1"
                     /translation="MSGPDDRRSTDREPGEDTPQAVVHVPKWSPWLWVIPVVAIFLAG
                     WLVVRYGIFGGGDITVRFADARGLERYSPVRYRGAKVGTVQKISIDKNLGQVSVRISM
                     DASMNHALRTGTRFWIVEPGLEGGGLGGLLSGTYIGIAPGDGDEVSEFVGQEDAPVLF
                     PAEAGKTFILESHGLGSVAVGSPLQYHGIRVGSILGSEYDAAHGVTFVHAFVAKRFEN
                     EVRQGTRFWRGGGLNLAFSGAGISSGGTSVSSLLNAPIAFYTPEVLPGPPVAEKTHFE
                     LYDSQAAAEAAADGPQLTYLTYFRGPVKGLVAGTPVQMNGVEVGRVRDVRLRYVPASA
                     TLESPVTLEIDPRKLELPVTPSTTRDEMRATMNDALQKLVQKGLRASLATSLVLPGAS
                     AVTLDMTGTAGSAHLVLTSDPPIIPSSAGSSGLEGALSAVNDVAARIRNLPIEEIAGH
                     LSNASQKMDALVSDPALHQSLQHLNASLADIEKITAATRENVGPITKSVRNAATSAEE
                     AAKSVNENIAPIAKSLRSAADSADTAAKRATQLVGSSQKQNYDLGTLIKELTDAAEAV
                     RALASYLTENPDALLKGRGK"
     gene            complement(3250..4461)
                     /locus_tag="VNN25_05500"
     CDS             complement(3250..4461)
                     /locus_tag="VNN25_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HXH91014.1"
                     /translation="MTLTCADCGRTQTIPPLPPGADAECQRCDRLLSHRSKIGLGVDL
                     PLACAIAILVLMPPAVFMPLMDSTIKNLVFQESRLVSSVPVIYGEVWFPFAFGFLFFA
                     FLFPAVRAALQIVVLGSIRVGKIVRHAGRFFRWSEELRIWSMTEVVVIAGIVAYYRAA
                     ISASVVLRVGAWCYLAVAIFAFIADRALDRRAVWSSILRDHTTAPVRHLASCGICEMA
                     VSTRRPGDPCPRCGHTLDSDAAKRYIPAVTAIAAAIPLLLPAYAFSVIVNDRITGVWE
                     HTVVGTVQLLADYGYWQVGVIMLIAGVLIPFLELVGMIWLLVTVRFPTRRGLVLRTRV
                     YRVLHRLVRWPMVIPFVAAMAAPIVEFKGIDEIVAGTGATPLFLFIALTMLAVRLFEP
                     KLMWRTAGGAW"
     gene            complement(4581..5978)
                     /locus_tag="VNN25_05505"
     CDS             complement(4581..5978)
                     /locus_tag="VNN25_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91015.1"
                     /translation="MSRMLMMSRLSMALSLLFAATWAMAIQPLKHERTFHIRQFDRSF
                     VVCGLRCPDNKENLVGGRLAPEMSFEMDPGESVEVWIDDPNPLLFTYKFGGITVVKNS
                     DVVALEAIATELSKVGGALQLKAGPSAKILASSRVWSDIDRKTQATSELERKITRLSA
                     AIKELARLQKEDVPTEIALSADPANWKKVRDNVESWDLSSLSQDVTDGYAAIDNAVEP
                     LDLTASTTPEILQALSNRSKVDALLKGLKDFADTVETIGVPIAITNTNGSPVFIEFDT
                     LNDQRFVVSIASLTGAQGFSQAAGDKLTAEHIPLKEDLTIIAIPHHNVHLRTAVAAVY
                     NFVRRPTFDANKSGDDLVIRRKDADRWTGQNVGLMLHIVPANWDQQFLSGEFQFGITP
                     IADQFAAYLGGGVVFNKYFSLATGIVAQRVDRLATGLHVGDVVASADDVKTVKRFQMG
                     AYICLTVDFKMPTGK"
     gene            6455..7264
                     /locus_tag="VNN25_05510"
     CDS             6455..7264
                     /locus_tag="VNN25_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH91016.1"
                     /translation="MNRKTIVQTAALIVVGIILALVANAFASRDRKLALVGSYPNALT
                     LPPHETAALPSQAVTETSPPTSMVTPSVTTAASTQTPASTTTAPATTTQPAATAAIET
                     VGLNPAAKNPAAKAPSSPPPVTASAPTPHSPLPTPPEDDPIKRFPPHPDKPYVEIAFA
                     EVNWLHGKKVLFLDARRTSVYEQGHISGARPYSVWESDIDDKVRKLFDERGDSQQSLP
                     IVIYCSGGECEDSHMLAQKLWLIQFNNIYVYKDGFPDWQQRGGPVHTGANP"
     gene            7261..7731
                     /locus_tag="VNN25_05515"
     CDS             7261..7731
                     /locus_tag="VNN25_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXH91017.1"
                     /translation="MKRLLTHPWLTIRVQIALGAIFVIAAWPKIADPPSFAHMIYNYR
                     LVPSSLINISSLVMPWIEILAGLALILGVWKTAARNVIIALLAVFIVAISINLMRDNA
                     IDCGCFNVADRGKSHEQRIFDMKVDVARDLGMLLLAAQLWYAGKRSDELDGIDN"
BASE COUNT         1579 a         2599 c         2274 g         1411 t
ORIGIN      
        1 gtacggtaat ggtgaggaca acatcgtcac cgcggtcggc acgttctcga tgtcgaccat
       61 caccgcctcc tcgagcgtcg ctgccagttc gaccggcaac acagcgtcgg tcactccatc
      121 caatggcacg acggtcacct atgcctggac gatcaccggc ggcacgatta ccagcgctac
      181 gaacatcgcc tccatcacat acaccgccgg cgcatcagga agcgtcgttc tacgcgccac
      241 ggcgtacaac acacgggcaa acaacggctc tggatttccc tccggaactg taggcgtgac
      301 agacacgaag accgtaacga tcgtcaccgt ctccccaccc accaacgtcg tggctgctgc
      361 gctcacctcg accagcgttc aggtcacatg gaccgccgcc tccggcgcga cgaaatacga
      421 gatctcgcgc tcggccaaca acagcacttt cacggccgtg tgcgtcgtgg cgagcacctg
      481 cccaacctct tcgccgttca ccgacagtac ggcatccgcg aacacggcgt acatctacaa
      541 agtaaagtcc gtcgacgccg gatcgaacct ttcagcattc agcaacggcg acctcgccac
      601 cacggtgatc ttcacggact ccctggtgca gaacacgacg aaagtgaagc tcgtacacat
      661 gaacgagctg aggacagcga tgaccgccgt ctgcacgctg gcggcgaatc cgtcgccttg
      721 cagcacagcc ttcaccgacc cgaccctgag tacatcgacc aaggtcagcg ttctgcacct
      781 gacggagctg cgcgcgaaac tcaatgccgc acgcagcacg cttggattga ccgcacagtc
      841 gtacgctgat ggatcgatca cgacggcgac acccatcagg gcggcacaaa tcaacgaact
      901 gcgcaacggg gtgcagtagc tgggacgact taccgcacct gccccacgat gtgatccgcg
      961 agcgtgccga tcgcgttgct gatgccggtg gccacgttcg cgctgtcgag gcttgggatg
     1021 tcggtgacga tgtgctcgcg ccgcgtgatg tccgcgcggc cggactcatg catcgtccag
     1081 cgtgcgtcga ggacgactgc gtgatccggc ccggcggcca gctcttcgac gaccacgtcc
     1141 agcgatcgca tgccgccgtc cggttgcgac tgacccggca gaatcaccat cccctcagga
     1201 agccgagaag ccagatcgaa cgccagcgtg tgcaggacga gcggttgcaa ggtcgtcgtc
     1261 cactgctcgc gtcctcgcac ctcgagttga tgattggcct gccggacgac gagctctttt
     1321 cgattgaggc cgggagggag cacgacgtcg atgccgatgg gaaggccgcg cacgacagcg
     1381 acgggcgcct gcgatgggat gcgatcgagc gaatagaacg tgctcggctt actggacagg
     1441 aagccgcagc cagccagcag caggggcacg aacagaatgc cgcggcgcat catcatttcc
     1501 ccctcccctt cagcagtgcg tcaggattct cggtcaggta cgacgccagc gcgcgcaccg
     1561 cttccgccgc atcggtcagc tctttgatca gagtgccgag gtcgtagttc tgcttttgtg
     1621 aagagccgac gagctgcgtg gctcgcttcg ccgcggtatc cgcggaatcg gcggcgctgc
     1681 gaaggctctt cgcgatcggc gcaatgttct cgttaactga cttcgccgcc tcctcggccg
     1741 acgttgccgc gttccgcacg ctcttcgtga tcggcccgac attctcgcgc gtcgccgccg
     1801 taatcttctc gatgtccgcc aacgacgcgt tcagatgctg caggctttga tgcagcgcgg
     1861 gatcgctgac cagcgcatcc atcttctgcg aggcgttgct cagatgtccg gctatctcct
     1921 cgatgggcag attgcgaatc cgcgccgcga cgtcgttgac cgccgagagc gcgccttcca
     1981 gtccgctgct gccggcagac gacggaatga tcgggggatc gctggtcagc acgagatgcg
     2041 cgctgcccgc tgttccggtc atgtcgagcg ttacggcgct cgctcccggc agcacgaggc
     2101 tcgtcgccag actggcgcga agtcccttct gcacgagctt ctgcagcgcg tcgttcatcg
     2161 ttgcgcgcat ttcatcgcgc gtcgtcgacg gcgtcaccgg aagctccagc ttgcgcggat
     2221 cgatctccag cgtcaccgga ctctcgagcg tcgcggacgc gggcacgtag cgcagccgca
     2281 catcgcgaac gcggccgact tcgacgccgt tcatctgcac cggtgtgccg gcgacgagtc
     2341 ccttcaccgg cccgcggaaa tacgtgagat acgtcaactg cggtccatcc gccgccgcct
     2401 cggctgccgc ttgcgagtcg taaagctcga agtgcgtttt ctccgcgact ggcgggcccg
     2461 gcagaacctc cggcgtgtaa aacgcgattg gcgcattcag caacgaggag actgacgtcc
     2521 cgccggacga gatgcccgcg ccggaaaacg ccaggttgag ccctccgccg cgccagaaac
     2581 gcgtgccctg ccgcacttcg ttctcgaaac gcttggctac gaatgcatgc acgaacgtca
     2641 cgccgtgcgc cgcgtcgtac tcggaaccta ggatgcttcc gacgcgaatg ccgtgatatt
     2701 gcagtggcga gccgactgcg acggatccga ggccgtgcga ctcgagaatg aacgtcttgc
     2761 ccgcctcggc ggggaagagc accggcgcat cttcctgccc aacgaactcg ctgacttcgt
     2821 cgccgtcgcc gggcgcgatt ccgatgtacg tgccggagag gagaccgccg aggccgccgc
     2881 cttcgaggcc gggctcgacg atccagaagc gcgtaccggt gcgcagcgcg tgattcatcg
     2941 acgcgtccat cgagatgcgc acggagacct ggccgaggtt cttgtcgatg ctgatcttct
     3001 gaacggtgcc gaccttcgca ccgcgataac gcaccggact gtatcgctcc agtcctcggg
     3061 catcggcaaa gcgaaccgtg atgtctcccc cgccgaagat tccgtagcgg acgacgagcc
     3121 agccggccag gaagatggcc acgaccggaa tgacccagag ccatggagac cacttcggaa
     3181 cgtggaccac agcctgcggc gtatcctcgc ccggttcccg atcggtactg cggcgatcgt
     3241 ctggcccgct caccatgcgc ctcctgccgt gcgccacatc aacttcggct cgaacaaccg
     3301 caccgccagc atcgtgagcg cgatgaacag gaagagcggc gtcgctccgg taccggcgac
     3361 aatctcgtcg attcccttga actcgacgat cggcgcggcc atggcggcta cgaacgggat
     3421 gaccatcggc cagcgcacga gccggtgcag aacacgatag acccgcgtac gcagaaccag
     3481 cccgcggcgc gtcggaaagc gcaccgtcac cagaagccag atcatcccaa ccaactcgag
     3541 gaacggaatg agcacgccgg cgatcagcat gatgacgccg acctgccagt agccgtagtc
     3601 cgccagaagc tgcacggtgc cgacgacggt gtgctcccag actccggtga tccggtcatt
     3661 gacgatgaca gagaaggcat acgcgggaag cagcagcgga atagcagccg cgatcgccgt
     3721 gaccgccggg atgtagcgtt tcgccgcatc gctgtcgagc gtgtgcccgc agcgcggaca
     3781 gggatcgccc ggccgccgcg tgctcaccgc catctcgcag ataccgcacg aagctaggtg
     3841 cctgaccggc gcggtggtgt gatcgcgcag aatcgagctc cagacagccc gccggtccag
     3901 cgcgcggtcc gcgatgaacg cgaagatcgc cacagcgagg tagcaccacg caccgacgcg
     3961 cagcacgaca ctcgccgaaa tggccgcgcg gtagtacgcc acgattccag cgatgaccac
     4021 gacctcggtc atgctccaga tccgcagctc ctcactccat cggaagaagc gccctgcgtg
     4081 ccggacgatc ttccccaccc ggatcgagcc gagcacgacg atctgcagcg ccgcgcgcac
     4141 tgccgggaag aggaaggcga agaaaagaaa gccgaacgcg aacggaaacc acacctcccc
     4201 ataaatcacc ggcacgctcg atacgagccg gctctcctga aagacaaggt tcttgatggt
     4261 tgagtccatc agcggcatga acacggcggg cggcataagg accaggatgg cgatcgcgca
     4321 tgcgagcggc agatcgacgc ccaatccaat cttcgaacga tgactgagca gccggtcgca
     4381 gcgctggcat tcggcgtcag cgccgggcgg aagcggagga atggtctgcg tcctgccgca
     4441 gtcagcgcag gtgagggtca tgagatcgct gccacgaagg ccatcaaaac aggcccatcg
     4501 gtgcaagagc gattcccaga aggagaaccg gggcctgtga cgattcaggc cccggaggtg
     4561 agaagtgctg ctattaaaga ctacttcccg gtgggcattt tgaagtcgac ggttagacag
     4621 atatacgcgc ccatctggaa acgcttgact gtcttcacgt catcagcgga ggcaacgacg
     4681 tcaccgacat gaagtcctgt cgccaaccgg tcgacgcgct gggcaacgat gcctgtagcg
     4741 agtgagaagt atttgttgaa cacgactccg ccacccagat acgcggcaaa ctggtccgcg
     4801 atcggtgtga tgccgaattg aaactctccg ctgagaaact gctggtccca gttggctgga
     4861 acgatatgca gcatgagacc gacattctga ccggtccatc ggtcggcatc tttcctgcga
     4921 atcactagat catcgccgga tttgttcgca tcgaacgtgg gccggcgaac gaagttgtac
     4981 acggccgcca ctgccgtcct cagatgcacg ttgtgatgag gaatggcgat gatggtgagg
     5041 tcctctttta gcgggatatg ttccgcggtg agcttgtctc cggccgcctg cgagaagccc
     5101 tgcgcaccag taagagaagc gatgctcaca acgaatctct gatcgttcag cgtgtcgaac
     5161 tcgatgaaga ctggactgcc gttcgtgttg gtgatggcga tgggcacgcc gatcgtttcg
     5221 accgtgtcgg caaaatcctt gaggcccttg aggagtgcgt caactttcga gcgattgctg
     5281 agggcttgga gaatctcagg agtggtactt gcggtgaggt cgagaggttc aaccgcatta
     5341 tcgatcgccg cgtaaccgtc ggtcacatcc tggctcaggc tgctgagatc ccaactctcc
     5401 acattgtccc gcactttctt ccaattcgcc ggatcggcac tcaaggcgat ctcagtaggc
     5461 acatcctcct tttgaaggcg cgcaagttcc ttgatggccg cacttagacg cgtaattttt
     5521 ctttcgagct cgcttgttgc ctgcgtcttc cggtcgatgt cggaccatac cctggaggag
     5581 gcgagtatct tcgccgaagg gcccgccttc aattgcagcg caccacccac cttcgacaac
     5641 tcggtcgcaa ttgcttccaa ggcaaccaca tcggagttct tcaccacggt gatcccgccg
     5701 aacttgtagg tgaatagcag cgggttcgga tcgtcgatcc acacttcgac cgactcgcct
     5761 ggatccatct cgaagctcat ctccggcgca agcctgccac ccacgagatt ctccttgtta
     5821 tcggggcagc ggagaccgca gacgacgaag ctccgatcga attgccgaat gtgaaacgtg
     5881 cgctcatgct tcagcggctg aatcgccata gcccacgttg ctgcgaaaag cagagatagg
     5941 gccatcgaga ggcgtgacat catcaacatg cgagacatgg tttctcctcg ttaccaaatt
     6001 ttggcgtagg tgacctgaag ctggtactca tctaggagtg tcgcgagcgg aacttttcct
     6061 accctcacgc atcagagacg gataccggag cgtttttcct tcctctgccg ccgtccaacg
     6121 cctttcgcat cgagatccat gggcggaaaa catatcgctc ctctcgatga acccggccac
     6181 ctgccagaga tccaacgctg tcgtcgccgg agaatggggg tcaggaatgc ccagtcctat
     6241 cggcaagagt tttcttgcgt cacttcacac tcagacctga ccccactccc gctccgactc
     6301 ccgacccccg aaaaccgacc cccgcataca ggacctgtag aatgcgctcg cggctgcttt
     6361 tcgagccgtg agtgagcatc cgatccgttg cccgctctcc gaagttcgca ccttcgcgaa
     6421 acgtcgcacg tttcactgcg tctaactatt gactatgaat cgaaaaacga tcgttcagac
     6481 cgccgccctc atcgtcgtag ggatcatcct cgcactcgtc gccaacgcgt ttgcctcgcg
     6541 cgaccgcaag ctcgcgcttg tcggttcgta cccgaacgcg ttgacgctcc cgccgcacga
     6601 gacggccgcc cttccttcac aggccgtgac ggagacgagc ccacccacca gcatggtgac
     6661 tccgagcgtc actactgctg cgtcaacgca gacgcccgcc tcaacgacga cggcgccagc
     6721 cacgacaacc cagccggcgg caactgctgc catcgagacg gtcggactga atcctgcggc
     6781 aaagaacccg gccgcaaagg ctccctcatc tccgccgccg gtgaccgcct ccgctcccac
     6841 cccccactcc ccactcccaa ccccccccga agatgatccc atcaagcgct tccccccgca
     6901 tccggacaaa ccgtacgtcg agatcgcctt cgccgaagtg aactggctgc acggcaagaa
     6961 ggttctcttc ctcgacgccc ggcgcacctc ggtctacgaa cagggccaca tcagcggtgc
     7021 gcggccttat tcggtgtggg agtccgacat cgatgacaag gtgcgcaaac tgttcgacga
     7081 gcgcggcgat tcgcaacagt cgctgccgat cgtcatctac tgcagcggag gcgagtgcga
     7141 ggattcgcac atgctggcgc agaaactgtg gctcattcag ttcaacaaca tctacgtcta
     7201 caaggacggc ttccccgatt ggcagcagcg cggcggaccg gtccacacgg gagcgaatcc
     7261 gtgaagcgtc tgctcaccca tccctggctc accatccgcg tccagattgc gctcggcgcg
     7321 atcttcgtca tcgccgcctg gccgaagatc gccgatccac cgtcgttcgc gcacatgatt
     7381 tacaactacc ggctcgtccc ttcgtcgctg atcaacatca gctcgctggt gatgccatgg
     7441 atcgagatcc tcgccggcct cgccctgatc ctcggcgtat ggaagaccgc cgcgcggaac
     7501 gtgatcatcg cgctcctggc ggtcttcatc gtggccatct ccatcaacct catgcgcgac
     7561 aacgccatcg actgcggatg cttcaacgtc gccgaccgcg gcaaatcgca cgagcagcgc
     7621 atcttcgata tgaaggttga cgttgcgcgc gacttgggga tgctcctctt ggcagcccag
     7681 ctttggtacg cgggcaagcg ctcggatgaa ttggacggaa ttgacaattg acaatggaca
     7741 attgacaacg aacaagtgtc tctcagggag cagcctcgcc tttcgcccgc attccattgc
     7801 cgatcgcaca aaccgtgttg tgtcatcctg agccgccgaa gccgccgcaa gattcacgac
     7861 act
//
LOCUS       DATLVW010000170         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_6175, whole genome shotgun sequence.
ACCESSION   DATLVW010000170 DATLVW010000000
VERSION     DATLVW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6175"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..261)
                     /locus_tag="VNN25_05520"
     CDS             complement(1..261)
                     /locus_tag="VNN25_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91018.1"
                     /translation="MRATYKAALWALAGWVSIVAYAQGPASSATAQTGATMASEASQP
                     GVSKKAERAANRAFAKKVHLALNKTKGLGGTEIAVFADARTG"
     gene            complement(410..712)
                     /locus_tag="VNN25_05525"
     CDS             complement(410..712)
                     /locus_tag="VNN25_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HXH91019.1"
                     /translation="MIRHIVMWRVAGRTASERRETAAHVRREFESLRGLIPGLLELVV
                     GLDVSEVDYACDVVLVADFESDAALDAYANHPEHLRVREALAGLRETRHQVDYYPG"
     gene            complement(979..1953)
                     /locus_tag="VNN25_05530"
     CDS             complement(979..1953)
                     /locus_tag="VNN25_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXH91020.1"
                     /translation="MDRYTQMEFFALTAELGSLSKAAERLGISNAAASRMLSALEDRL
                     GTRLVERTTRRLWLTDAGREYRQRCASILADIAEADTAVSDMAWRPRGVLRVTSSVSF
                     AVLHIAPWLPEFRQRYPELSVHITAANRYPNFIEAGIDCAIRTREHEGDSGITVRRLA
                     HTRRVLAASPAYLAKHGMPEKPEDIARQNVLVYSYATNPYLLQFKRGRERRNIPINAV
                     LESNEGQVICAAARAGLGIVVQPLYIVHDDIVAGRLVPLLGDWELPPLTINLAYQSRQ
                     NQPAKIRVFADALTEHVRTLDLENRWNAMPKIAVAARIAPKTRVSDSR"
     gene            2086..>2689
                     /locus_tag="VNN25_05535"
     CDS             2086..>2689
                     /locus_tag="VNN25_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HXH91021.1"
                     /translation="MRNINEDTITQAVIAAMSDCGNDRLRTVMTSLVQHLHSFAREVK
                     LTESEWLYAINFLTDVGHITDDKRQEFILLSDTLGLSMLTTTQNNRKPSECTEATVLG
                     PFFVEGAPEYQNGDDISNGASGLPCFVTGRVRGLHGEPVSGAALEVWQSDEDGNYDVQ
                     YAAQHQGDVEHRGRGRLHTLADGRFHFRSILAEAYPIPHDG"
BASE COUNT          557 a          840 c          747 g          545 t
ORIGIN      
        1 ctaaccggtt cgcgcatccg cgaaaaccgc gatttcggtc ccacccaagc ccttcgtctt
       61 gttcaacgcc aggtgaactt tcttcgcaaa cgcgcggttc gcggcacgtt cggccttctt
      121 cgagacgccg ggttgcgacg cttcactggc catcgtggcg ccggtttgag ccgtcgcgga
      181 ggacgccggc ccttgggcat atgcaacgat agaaacccat cccgccaacg cccacaacgc
      241 cgccttatat gtcgctctca ttggcttacc ttgctatttt caaagattaa aattgctcgg
      301 cacatgtatc gggtctttct tcgaaacacg gtgcccgctc cgacttcgtt gttgccccca
      361 cctcgagggc tcatccttac cgcggtgtaa ttagttgaag acagccgccc tagccgggat
      421 agtagtcaac ctgatgacgt gtctcccgca agccagcaag tgcttcgcga actcgaagat
      481 gttcggggtg gttcgcatag gcgtccaagg cagcgtccga ttcaaaatcg gccaccagga
      541 cgacgtcgca cgcgtagtcg acctcgctga cgtcgagtcc cacaacgagc tcgagcaagc
      601 ccggaatcaa gcctcgaagg ctctcgaact ctctcctgac atgagcggcc gtttcgcggc
      661 gttcgctcgc agtccggccc gcaacacgcc acatcactat gtgtctgatc atcgcttcgt
      721 ctcacgaagt ggattcaaaa tgaagtagtc gcctgattat attttccgat cgccaagttt
      781 aaaaaaagca taagccgatc ttcggcgaag aaaaatgcgt gtgctggaaa attgacttcc
      841 agtaagtgga agaatcgatc gaagcgatca ggcacagttc aacttcgccc ggcttggtgc
      901 gagcgctccc tgtcgcgagc gatagggctt cagccctcgg ctcaggcgag ccttgcgcgg
      961 cgctccgtcg gtgccgcgct accgggaatc ggaaacccgg gttttcggcg cgatccgtgc
     1021 agcaacggca attttaggca tcgcgttcca gcgattctcg aggtcgagcg tcctgacatg
     1081 ctcggtcaac gcatcggcaa aaacccgaat cttcgcgggc tggttctgtc ggctctggta
     1141 cgcgaggttg atcgtcagcg gcggaagctc ccagtcgccc agcaacggca ccagccgacc
     1201 ggcgacgata tcgtcgtgca cgatgtacag cggctgaact acgatcccca gtcctgcgcg
     1261 ggccgcggcg cagataacct gtccttcatt ggactccagc accgcattga tcggaatgtt
     1321 gcgccgctcc ctgcctcgtt tgaattgcaa cagatagggg ttcgtcgcgt agctataaac
     1381 gagcacgttc tgacgggcaa tatcttcagg cttttccggc atgccatgtt ttgccagata
     1441 cgccggtgac gcggccagca cacggcgagt gtgcgctagc cgccggacgg taatgccaga
     1501 atctccctca tgttcgcgcg tgcgaatcgc acaatcgatt cccgcctcga tgaaattcgg
     1561 ataccggttc gccgccgtga tatggacgga tagctctgga tagcgttgtc tgaattccgg
     1621 aagccatggc gcgatatgca agaccgcgaa agacacggaa ctcgttacac gcagcactcc
     1681 ccgcggtcgc catgccatgt cactgacagc cgtatccgct tcggcgatgt ccgcgagaat
     1741 cgaagcacaa cgctgccgat actcacggcc cgcatccgtc agccacagcc ggcgagtcgt
     1801 acgctcgacc agacgtgtcc ccaaacgatc ctcgagcgcg ctcaacatcc gactcgccgc
     1861 tgcattcgaa atgccgagcc gttccgcggc tttggacagg ctgccgagtt ctgctgtcag
     1921 cgcaaagaac tccatctgcg tgtagcgatc catcggatga tttttccgct caaaggaaaa
     1981 ttgttttcac tatagcgcat attttttctt cctgaacctc gctagagtgc cgacatggcc
     2041 gctcctcggg agtgagatca gtcagcacag atgcggagac aggcgatgcg caacatcaac
     2101 gaagacacca tcacacaggc agttatcgca gcgatgtcgg actgcggcaa tgacaggctg
     2161 cgcacggtca tgacgagctt ggtccagcat ttgcactctt tcgcaagaga ggtcaagctg
     2221 acagagagcg agtggctcta cgccatcaac ttcctgaccg acgtcggcca tatcaccgac
     2281 gacaagcgcc aggagttcat cctcctgtcg gatacgctcg gcctgtcgat gctgacgacc
     2341 acgcagaaca accgcaagcc ctccgaatgc acggaggcca cggtgctggg gccgttcttc
     2401 gtcgaagggg cgcccgaata ccagaacggt gatgacatct ccaacggcgc ctcaggcctg
     2461 ccgtgcttcg tcacgggccg ggtccgcggt cttcacggcg agccggtgtc cggtgcggcg
     2521 ctcgaagtct ggcaatcgga cgaggacggc aactacgacg tgcaatacgc cgcccagcat
     2581 caaggcgatg tcgagcatcg gggccggggc cgcttgcaca cgcttgccga cggacgcttt
     2641 cattttcgct cgatcctcgc agaggcttat ccgatcccgc acgacggtc
//
LOCUS       DATLVW010000171         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_6176, whole genome shotgun sequence.
ACCESSION   DATLVW010000171 DATLVW010000000
VERSION     DATLVW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6176"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VNN25_05540"
     CDS             complement(<1..1489)
                     /locus_tag="VNN25_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH91022.1"
                     /translation="MKNDSMLLRCGHLVLLIVALLGFVPSLAAQSTYQLPPKEVVDAF
                     DAAPLPEATLSPSKKVIALSSRRGQPSISEIAQPMLRLAGARINPNNFGQHRAGLIYA
                     IKLKAIAGGAEIDVKTPPDAHIANIRFSPDGSKLAFLNTRSDGIDLWVADVATGKTTL
                     ATNHANSTAGDVFDWMPDNATLLCKVVPEGRGPAPSEPVTPAGPNVQVSEGKSAQAAT
                     YEDMIRTAHDEALFEYYFTAQLALISADASKTTLVGKPAIFGGVSPSPNGDLILVTKI
                     KRPFSHLIPMNGFPEDVEVWDRKGSVIKQLADRPSREGVPLTGVEPGPRGHHWRLDQP
                     ATIVWTEALDGGNPKNKVPFRDRVVSLAAPFSGQPVELGKTEFRSGGILFTEKGVALL
                     TEFDRVSRRIRTWILEPGAEPKKLWDRKQDAAYENPGNPVMRIDGSDNRGFGGRDGLV
                     IQTGDSIYLTGQGASKEGDHPFFDRLNLKTLATERLFHSAPDAYEN"
     gene            complement(1569..>2084)
                     /locus_tag="VNN25_05545"
     CDS             complement(1569..>2084)
                     /locus_tag="VNN25_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91023.1"
                     /translation="AARSEGVGKGSEFVVRLPLAAYDEQHGSGSAAAPKERETEVHAA
                     RRIAIVEDNKDVRDLLRLKLSRLGHTVVDARDGLEGLRIVLAERPDLALVDLGLPGID
                     GFQLAKEVREQLGDDVILVAVSGFGQPDDKRRAIEAGFDEHLTKPADVHDIENLLMRL
                     PPRNARAAEIG"
BASE COUNT          330 a          639 c          702 g          413 t
ORIGIN      
        1 cgttctcgta agcgtcgggc gcgctgtgaa agagccgttc cgtggcgagc gtcttcagat
       61 tcaggcggtc gaagaacgga tgatcgcctt cctttgacgc gccctggccg gtcagataga
      121 tcgagtcgcc ggtctggatc accaggccgt cgcgtccgcc gaagccgcgg ttgtcgctgc
      181 cgtcgatgcg catgaccggg ttgcccgggt tttcgtacgc cgcgtcctgc ttgcggtccc
      241 acagcttttt cggctctgcg cccggttcga ggatccacgt ccgaatgcgc cgcgagacgc
      301 ggtcgaattc agtgagaagg gcgacgcctt tctccgtgaa caggatgccg ccggagcgaa
      361 actcggtttt tccgagctcg acgggctgcc cggagaacgg tgcggccagc gacaccacgc
      421 gatcgcggaa cgggactttg tttttcggat tgccgccgtc gagcgcttcg gtccagacga
      481 tcgtcgccgg ctgatcgagc cgccagtgat gtccgcgcgg tccgggctcg acgccggtca
      541 gcggcacgcc ttcgcggctc gggcggtcgg ccaattgttt gatgaccgag cccttccgat
      601 cccacacctc gacatcttca gggaagccgt tcatcgggat gagatgcgag aaaggacgct
      661 tgatcttcgt gacgagaatg agatcgccgt tgggcgacgg agatacgcct ccgaagatcg
      721 ccggcttgcc gacgagcgtg gtcttgctcg cgtccgcgct gatcagcgcg agttgcgcgg
      781 tgaagtagta ctcgaacagc gcctcgtcgt gcgcagttcg gatcatgtct tcgtacgtgg
      841 ctgcctgcgc gcttttgcct tcgctcacct gaacgttggg gccggccggc gtcaccggtt
      901 ccgagggcgc cggaccgcgg ccttcgggaa cgaccttgca gagcagcgtg gcgttgtcag
      961 gcatccagtc gaagacgtcg ccggccgttg aattggcgtg gttggtggcg agcgtcgtct
     1021 tgccggtggc cacatcggcg acccagagat cgattccatc gcttcgcgta ttgagaaacg
     1081 ccagcttcga gccgtcggga gagaatcgga tattggcgat gtgggcatcc ggaggcgtct
     1141 tcacgtcgat ttcggcgccg ccggcgatcg ccttcaactt gatcgcgtag atgagccccg
     1201 cgcgatgctg gccgaaattg ttcggattga tgcgtgctcc cgccagccgc agcatcggct
     1261 gggcgatctc gctgatcgat ggctggcctc gccgcgacga cagtgcgatg accttcttcg
     1321 acgggctgag ggtcgcctcc ggcagcggcg cggcatcgaa agcatcgacc acttccttcg
     1381 gcggcaactg gtacgttgat tgtgcggcca gcgaggggac gaacccgagt agcgcgacga
     1441 tcaggaggac cagatggccg cagcgaagga gcatcgaatc gttcttcatg gcgcggattg
     1501 tagcggacgg accgatgcgt gatgcgtgac gattcgaacg ccgtggccgc gagaagagct
     1561 ggcgatgact acccgatttc ggcggcgcgc gcattccgcg gtggcaggcg catcagcaaa
     1621 ttctcgatgt cgtgcacatc ggctggtttc gtcagatgtt catcaaaccc ggcttcgatc
     1681 gcgcgccgct tgtcgtcggg ctgaccgaag ccgctgacgg cgacgaggat gacgtcgtcg
     1741 ccgagctgct cgcggacttc cttcgccagt tggaagccgt cgattccggg aaggccgagg
     1801 tctacgagag cgagatcggg gcgctcggcg aggacgatac gcagtccttc caggccgtcg
     1861 cgcgcatcga cgactgtgtg gccgagccgg ctgagtttga ggcggaggag atcgcgaaca
     1921 tccttgttgt cttcgacgat ggcgattcgc cgtgcggcat gcacctccgt ctctcgctcc
     1981 ttcggagcgg cggcgctgcc gctgccgtgt tgctcgtcgt atgccgccaa cggcaagcgg
     2041 accacgaact cgcttccctt gcccacgcct tcgcttcgtg ccgc
//
LOCUS       DATLVW010000172         6504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_134557, whole genome shotgun sequence.
ACCESSION   DATLVW010000172 DATLVW010000000
VERSION     DATLVW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134557"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VNN25_05550"
     CDS             <1..1443
                     /locus_tag="VNN25_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXH91024.1"
                     /translation="LLQPASFALIQNEILLLKAIGVKAVSVEVSFPMLDADFFNSIGQ
                     PGYQSQFTTFYANVAAAIRAQGLQVIVESQSMIPTGLQSSWGSGLQNYYASLTTFQAY
                     ENARSQTAGVVAQTMHPDYFVLQEEPDTEATQSGQASAGTVAGSTTMLNGSLTAARQA
                     NVAGMKIGAGLGSWLQQYQLFANSFTLQHCGQTVSGQTQPCISQPLDFLDLHLFPITE
                     NAVDCTPPPAPKACTAPNFWQNAMNILSTANAASVPMAISQTWLRKVRDSEWVQINGD
                     IQEAREAYSFWAPLDLAFLQDVYDFANYGQMLFVVPFNTQNFSAYIDWTGGNSTCSVS
                     GTPACTAQQGEGGGNTPSQVFGAVQSAGTTNVAAARYSSVGIGYHNLIVSDTVAPRNP
                     TNVFVQRPAPTTATVSWSPSTDDVGVAGYHIFRNGTPLRDVFQSPLQDTGLSSTTNYT
                     YQVQAFDLAGNVSAAVSASTQNGRRRAVAH"
     gene            complement(1545..2996)
                     /locus_tag="VNN25_05555"
     CDS             complement(1545..2996)
                     /locus_tag="VNN25_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXH91025.1"
                     /translation="MRIITDDRYIRTYSYVVLHLTAALLFSLTMQSTAPAAEHYKLRF
                     AIDPKTHSLSAGADLGIRNTEARAITLVPVLLYRLMDVSAASDGAGNPLRFAQKVVKF
                     PDEPTWQVNAVEVTLAKPLPPGQTTTVHLTYAGPLFGYPEVMQYVRDTISEQYSLIRA
                     ETMAYPIVAPASIAGWRQSFKNKFDFEIETKVPAGFTAVCNGKDVGEAQTQEGTSTFR
                     CIGDAGSAQISVAVAKFRVLDDPERNLRVYALDADAEAGARVMDDMRRSLDFYRSYFG
                     EMRRGGSLTLVEIPDGWGSYGVPGHIFQSAAAFKDRSRASELYHEVGHIWNARASDDV
                     QRARYFDEAFASYFEALAVRQFEGPDAFRALMRSYRQTYLDRVARDARGRTVPISGYG
                     KEEIGVFSYTKGAWSLYVLHQLLGEDAFRRAISDFLTAYSDKPAGFDDFRHAVEKSSG
                     HDVGPWFEQWITGTNASAMLIDGKSVEEMGGKR"
     gene            3080..3826
                     /locus_tag="VNN25_05560"
     CDS             3080..3826
                     /locus_tag="VNN25_05560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH91026.1"
                     /translation="MTVQERAERLDRSTFFGVPIAGFEKAGRQQFVLLLMAGLEPHSK
                     VLDLGCGVLRAGYWLIHFLDPHCYFGIEPHRERLETGRTLLLEPDTERVKEPRFDTNP
                     EFDSSVFGVKFDFFLAYSIWTHASKPQIEMMLDNFLRDSTPGAVFLTSVLPAVWPRRD
                     YGRKGWFGTSHELGQPGCIRHSLKWIDDACRRRGLRVNNLGRERDGQTWLQISRESGA
                     RLLFRTIWAEWFPVRLSRRLFEKVGRRRRA"
     gene            complement(3827..4951)
                     /locus_tag="VNN25_05565"
     CDS             complement(3827..4951)
                     /locus_tag="VNN25_05565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH91027.1"
                     /translation="MRVLIAGHSLGEIGGVQSYERDLASWLLARGHSPVVYATELGDA
                     ARQLNKLTVPVVDDLSSITAPIDIIHGDSAIETMAALLHFSTTPAIFVCHGWEDLGRT
                     TPRFPRTLRYVAVDDTCADRLVTREGIAPERISVLLNAVDMSAFRQREPLPDRPRRAV
                     VFGNLAHELTFLPAIRAACRRASIELDVVSASAGTAVANPESILGGYDLAFAKAKCAM
                     EAMACGLAVILCSEAGLGGTVRSIDFDRLRRLNFGIRTLQNPLTTETVLAELALYDAD
                     DARRVSDRIRESAPSDALHESLLSLYQSVIDEHAGMSSKNDGVAESRAASAFLHALAT
                     RERHREGRMWMLANAAQRVLQVPVVGPMGTRLLRWLAPKR"
     gene            complement(4939..5682)
                     /locus_tag="VNN25_05570"
     CDS             complement(4939..5682)
                     /locus_tag="VNN25_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91028.1"
                     /translation="MVHLLVAHPGHELLLHGWISRNKPVVHVLTDASAHSSDARLSRT
                     AELLRGLGARKGAIFGRLTDAEAYAMILERNTPLLASLVAELAEQLEEDQPAIIVGDA
                     AEGYNPVHDLCRLIAGAAIAMAGVSTKQYEYAVVNHPHSSDATIVLDLDAVEYAAKME
                     RAREQAAALNDIETLLSRHGADAYRTEALYPVADWTAVGGDEPPLYERFGEERVAAHR
                     YTQVIRRRQHMLPLRDALCAEVEKRSCAF"
     gene            5776..6066
                     /locus_tag="VNN25_05575"
     CDS             5776..6066
                     /locus_tag="VNN25_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXH91029.1"
                     /translation="MKKLLGSIIMSAALVASLGCSNWNRMTPKSLDNSTTASEIKKNL
                     LADHLTGVDVDVTEGVATLSGHLPTKADREKAYDDAAKVTGVKKVVNHIDVP"
     gene            complement(6044..>6504)
                     /locus_tag="VNN25_05580"
     CDS             complement(6044..>6504)
                     /locus_tag="VNN25_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HXH91030.1"
                     /translation="SIWFTTHRDVHPEGARMDEVLADMIAYAGAIKTADAGALVLGPE
                     EWRWSGYFYSGYDQQWGAAHDWSSLSDRAGLAIDLAERDYAARRCLPGRVECGHHGAA
                     AEHHAGCSHLLKQRAASPRGESLMNTPRRTRVRLFRSVTTKTALTARRCG"
BASE COUNT         1254 a         2008 c         2043 g         1199 t
ORIGIN      
        1 ctgctgcagc ccgccagttt tgccctcatt cagaatgaga tccttctcct gaaggcgatc
       61 ggtgtaaagg ccgtctcggt ggaggtgtcc ttcccgatgc tggacgccga tttcttcaac
      121 tccatcggtc agcctggtta ccaatcgcaa ttcacaacct tctacgcaaa cgtggcggcg
      181 gcaatccgcg cccaggggct gcaggtgatc gtcgagagcc agtcgatgat ccccacgggc
      241 cttcaatcgt catggggcag cggcttgcag aattactatg caagcctgac caccttccag
      301 gcctacgaga atgcccggtc acagacggcc ggtgtcgtcg cccaaacgat gcatcccgat
      361 tacttcgtgc ttcaggagga gccggacacc gaggccactc agtcggggca ggcgtcggcc
      421 gggactgtgg ccggttcgac aacgatgttg aatggcagcc tgacagcggc cagacaggcc
      481 aacgtcgccg ggatgaagat cggcgcaggg ctcggctcgt ggttgcagca gtatcaattg
      541 ttcgcgaaca gcttcacgct ccagcattgc ggtcaaaccg tcagcggaca gacgcagcct
      601 tgcatttccc agccactcga cttcctggat ctgcatctct ttccgatcac cgagaacgcc
      661 gtggactgca cgcctccacc ggccccgaag gcatgcaccg ccccaaactt ctggcaaaac
      721 gccatgaaca tcctcagcac ggccaacgcg gcgagcgtgc cgatggccat ctcccagacg
      781 tggctccgaa aggttcgcga cagcgaatgg gtccagatca acggcgacat tcaggaggcc
      841 cgcgaggcgt acagcttctg ggcaccgctc gatctggcgt ttctgcaaga tgtgtacgac
      901 ttcgcgaact acggccagat gttgttcgtc gtcccgttca atacgcagaa cttctccgcc
      961 tatatcgact ggaccggcgg caactcgacg tgcagcgtta gcggcacgcc ggcgtgtacg
     1021 gcgcagcaag gtgaaggcgg cggcaataca ccgtcccagg tctttggcgc cgttcagtca
     1081 gccgggacca ccaacgttgc tgccgcgcgt tacagcagtg tggggatcgg gtatcacaac
     1141 ctgatcgtgt ccgacacggt ggcgcccagg aatcctacca acgtgtttgt gcagcggccg
     1201 gctccaacca cagcaaccgt gagctggtca ccgtcgaccg acgacgtcgg cgttgcggga
     1261 taccacatct tccgtaacgg gacaccgctt cgcgacgttt ttcagtcacc gctgcaggac
     1321 acgggccttt catcgacaac caattacaca taccaggtgc aggccttcga tctggcagga
     1381 aatgtctcgg ccgcggtgtc ggcgtccacg caaaatggac ggcgaagagc ggtcgcgcat
     1441 tagcaacgag ccaactctaa tcgcctgcaa gcgggagaaa tgcggttagg ttgagtgtca
     1501 tccggagtga ttgtccaatc gagacagaac acgttttggt gcgcctagcg cttaccaccc
     1561 atctcctcga cgctcttccc atcgatcagc atcgcagatg cgttggttcc tgtgatccat
     1621 tgttcgaacc aggggcctac gtcgtggccc gacgacttct cgacggcgtg gcggaagtcg
     1681 tcgaagccgg ccggcttgtc gctgtacgca gtgaggaaat cggagatcgc gcggcggaat
     1741 gcgtcttcgc cgagcaactg atgcaggacg taaagggacc aagcgccttt ggtgtacgag
     1801 aagactccga tctcttcttt gccgtagccg gagattggaa ccgtgcggcc tcgcgcatcg
     1861 cgcgccactc gatcgaggta cgtctgccgg taactccgca tcagcgcgcg aaacgcgtcc
     1921 ggtccctcga actgccggac cgccagcgct tcgaagtacg aggcgaaggc ttcgtcgaag
     1981 taacgagcgc gctgcacgtc gtcgctcgcc ctggcgttcc agatgtggcc aacctcgtga
     2041 tacagctccg acgcccggct gcggtccttg aaggctgccg cgctctggaa aatgtgcccg
     2101 ggcacgccgt acgagcccca gccgtcgggg atttcgacga gcgtgaggct gccgccgcgg
     2161 cgcatctcgc cgaaatacga tcgatagaaa tcgagagagc gccgcatgtc atccatcacc
     2221 cgcgcacccg cttccgcatc ggcgtcgagg gcatagaccc gcaaattacg ttccggatcg
     2281 tcgaggacgc ggaacttcgc cacggctacg ctgatctgcg cgcttcctgc atcgccgatg
     2341 cagcggaacg ttgaagtccc ttcctgcgtc tgcgcctctc cgacgtcctt tccgttgcag
     2401 acagcggtga agccggccgg cactttcgtt tcgatctcga agtcgaattt attcttgaac
     2461 gactgccgcc agccggcaat cgaggcaggt gccacgatcg gatacgccat cgtctccgct
     2521 cgaatcaacg agtactgctc gctgatcgtg tcccggacgt actgcatgac ttcggggtag
     2581 ccgaagagcg gccctgcgta ggtcaaatga acggtcgttg tctgcccggg aggaagcggc
     2641 tttgcaagcg tcacttccac ggcgttcacc tgccaagttg gttcatctgg aaacttgacg
     2701 accttctgcg caaaacgcag cgggttgccc gcaccgtcgg aagcggcgct gacatccatc
     2761 agccggtaga gcagcacggg gacaagcgtg atcgctcgcg cctcagtgtt gcggatgccg
     2821 aggtcggctc ccgcggagag cgaatgagtc ttcggatcga tggcaaagcg gagcttgtaa
     2881 tgctccgcgg cgggcgccgt actttgcatg gtcaaggaga agagaagggc tgcggttaga
     2941 tggagcacga catacgaata tgttcgtatg taccggtcgt cggtgatgat gcgcaccggt
     3001 cgatgtttgc cttgttactg agtacgccgc gcaggtgcgg atgtccatca ggcggcagtg
     3061 ctgtgatcca atctcaccga tgacagttca ggagcgcgcg gagcggcttg accgttcgac
     3121 gtttttcggg gtgccgatcg cggggttcga gaaggcggga cggcaacagt tcgttttgct
     3181 cttgatggcg ggcctggaac cgcactcgaa ggttctcgat ctcggttgtg gagttctacg
     3241 ggcaggatac tggctcatcc acttcctcga tccgcattgc tactttggga tcgagccgca
     3301 ccgtgagcgg ctcgagacgg gaagaacctt gctcctggag ccggacaccg agcgcgtcaa
     3361 agagccgcgg ttcgatacga atccggaatt cgacagcagt gtcttcggcg tcaaattcga
     3421 tttcttcctc gcctattcga tctggacgca cgcttcgaag ccacagattg aaatgatgct
     3481 cgacaacttt ctgcgagact cgacgccggg cgcggtgttt ctcacctccg tgctgccggc
     3541 cgtctggccg cggcgtgact atggtcggaa aggatggttc ggcacgagcc acgagctggg
     3601 acagccgggt tgcatccggc acagtctgaa gtggatcgac gatgcgtgtc gtcgccgcgg
     3661 cctgcgcgtg aacaacctgg gccgagagcg cgacggacaa acgtggcttc aaatctcgcg
     3721 cgagtccgga gcgagactct tgttcagaac gatctgggcg gaatggttcc cggtcagatt
     3781 gagccggaga ttgttcgaga aagtcggccg tcgccgtcgc gcgtaatcat cgtttcggag
     3841 ccagccagcg aaggagccgg gtacccatcg gtccgacgac cggcacctgc agcacgcgtt
     3901 gcgcggcatt cgccagcatc cacatccgac cttcccggtg gcgctcccgg gtagccagag
     3961 catggagaaa cgccgaagcc gcgcggctct cggcgacccc gtcgttcttc gaagacatac
     4021 ccgcatgctc gtcgatgacg ctctggtaca acgacaggag cgactcgtgg agcgcatccg
     4081 atggcgccga ctcgcgaatt cgatccgaga ctctgcgggc atcatcagcg tcgtacagcg
     4141 cgagctccgc aagaacggtt tccgtcgtca gcggattctg aagcgtgcgg atgccgaagt
     4201 tgaggcggcg caggcgatcg aagtcgatcg aacgaaccgt gccgccgagg ccggcctcgc
     4261 tgcagagaat gacggccagt ccgcacgcca tcgcctccat tgcgcacttc gccttcgcaa
     4321 acgccaggtc gtagccgccg aggatcgact ccgggtttgc cacggctgtt ccggcggagg
     4381 cgctgacgac gtcgagctcg atggacgccc ggcggcaggc cgcgcggatc gccggcagaa
     4441 acgtgagctc gtgcgcgaga ttgccgaaga ccaccgcgcg ccgcggccgg tccggaagag
     4501 gctcgcgctg ccggaaggcg ctcatgtcga ccgcattgag cagcaccgaa attcgttcgg
     4561 gcgcaatccc ttcgcgcgtc acgaggcggt cggcgcaggt atcgtcgacg gcgacgtagc
     4621 ggagggttcg ggggaatcgc ggcgtagtcc ggccgaggtc ttcccagccg tggcaaacga
     4681 agatggccgg cgtggtcgaa aaatgaagca gcgcggccat ggtctcgatc gcgctgtcgc
     4741 cgtggatgat gtcgatcggc gcggtgatgc tgctcaaatc gtcgacgaca ggaacggtca
     4801 gtttgttgag ctggcgcgcg gcatcgccga gctccgtggc ataaacgacc ggcgaatgcc
     4861 ctcgggcgag cagccacgac gcgaggtcgc gctcataact ctgcacgccg ccgatctcgc
     4921 cgagcgaatg gccggcgatc agaacgcgca tgagcgcttt tccacctcgg cgcacagcgc
     4981 gtcgcgcaac ggaagcatgt gctggcgacg acggatcact tgcgtgtacc ggtgcgcggc
     5041 gacccgctcc tcgccgaaac gttcgtagag cggtggttcg tcgccgccca cggccgtcca
     5101 gtcggcgacg ggataaagcg cctcggttcg ataggcgtct gcaccgtgtc gcgacaacaa
     5161 cgtttcgatg tcgttcaacg cagcggcctg ctcgcgcgcc cgctccatct tggcggcgta
     5221 ttcgacggca tccagatcga gaacgatcgt cgcgtcggac gaatgaggat gattgaccac
     5281 ggcgtactcg tactgtttcg tggagacgcc ggccatggcg atcgcagcac cggcgatgag
     5341 gcggcagaga tcgtgaaccg ggttgtagcc ttcggcagcg tcgccgacga tgatcgcggg
     5401 ctgatcctct tccagttgtt ctgcgagctc cgcgacgagc gaggcgagca ggggagtgtt
     5461 gcgctcgagg atcatcgcgt acgcctcggc gtcagtaagc cttccgaaga tcgcgccttt
     5521 gcgggcgccg aggccgcgca ggagctccgc cgtccggctg agacgcgcgt cggaagagtg
     5581 cgccgaagcg tcagtcagga cgtggacgac cggtttgttc cgggaaatcc aaccatggag
     5641 gagaagttcg tgacctggat gagcgactag tagatgaacc atcgggcgga gggaaattat
     5701 acgcgagcgc cgcactctcg tttgcgcgga gcctggcttg cctattgctc cccgcccagt
     5761 caaaggagta acgagatgaa gaagttgctt ggatccatca tcatgtccgc ggcgttggtc
     5821 gcgtccctcg gctgctcgaa ctggaacagg atgactccga agtcgctcga caattcgacg
     5881 acggcgtcag agatcaagaa gaacctgctg gccgaccacc tgaccggcgt cgatgtcgat
     5941 gtcaccgaag gcgtcgcgac gctgtccggt cacctgccca ccaaagcgga tcgcgagaaa
     6001 gcatacgacg acgcagcaaa ggtcaccggc gtgaagaagg tcgtcaacca catcgacgtg
     6061 ccgtaagggc tgtcttcgta gtcactgaac gaaagaggcg gacccgcgtc cgcctcggcg
     6121 tgttcatcag cgattcaccg cgcggcgacg cggcgcgctg tttcagcagg tgggaacacc
     6181 cagcgtgatg ctctgcggcg gcaccgtgat gaccgcactc gacccggccg gggagacatc
     6241 gtcgagccgc gtaatcgcgt tcagcgaggt cgattgccag acctgcgcga tcagacaagc
     6301 tcgaccaatc atgtgccgct ccccactgct ggtcataccc actgtaaaaa tacccgctcc
     6361 atcgccactc ttcgggaccg aggacgagag cgccggcatc ggccgtcttg atcgcgcccg
     6421 catacgcgat catgtccgcc aacacttcgt ccatccgtgc cccctcgggg tgcacatcgc
     6481 gatgcgtggt aaaccagatc gacg
//
LOCUS       DATLVW010000173         3589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_88868, whole genome shotgun sequence.
ACCESSION   DATLVW010000173 DATLVW010000000
VERSION     DATLVW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88868"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VNN25_05585"
     CDS             <1..550
                     /locus_tag="VNN25_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH91031.1"
                     /translation="SYLIQRTDGNVLIDSPRFSGPLVKNIDSMGGVSKLLLTHQDDVA
                     DHAKFHDRFGCERVLHRDDVHSRTASIELQPAGIDPISLGNEIVMIPTPGHTKGHAVF
                     LYRDRYLFTGDHLAWSERRGHLYAFRSACWYSWSEQIRSMKRLLDYRFEWVLPGHGRP
                     VHLDPAAMRASLERCVVWMKAN"
     gene            646..1665
                     /locus_tag="VNN25_05590"
     CDS             646..1665
                     /locus_tag="VNN25_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXH91032.1"
                     /translation="MKYFALLKQTISEFGNDKAPRLGAALAYYTIFSLAPLLLIVIAI
                     SGLAFGHDAAQNKIFEQLRGVLGPQAAVSVQDMVKNAAKPKSGTVATIIGIVTLIFGA
                     SGVFGQLKDALNTIWDVKPREGGGILTLVKDRFLSFAMVLGVGFLLLVSLVIDTAITA
                     IGTYAGSHLPGGEVLWHAAELIFSFCVITVLMAMMFRLLPDLKIEWRDVWLGAAITSL
                     LFVLGKFALGIYFAKSAVGSSFGAAGSLVIVLLWVYYSAQILLFGAEFTQVYAHAHGS
                     LKGAKQSEAPQPAPIPVVVLKPSAAGGAAKLLTGGAAGLIIGLIAGLVGAIVVFVKSA
                     KKLVT"
     gene            complement(1672..1941)
                     /locus_tag="VNN25_05595"
     CDS             complement(1672..1941)
                     /locus_tag="VNN25_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91033.1"
                     /translation="MNVKSPTRTTMTRLIPPLAVWAIGRILDSPSVKGTVMQLDGETY
                     KRRHDAVRAVRKGLKNARSNASWVAAGAAAIAIGIGLVAKAASSK"
     gene            complement(2169..2765)
                     /locus_tag="VNN25_05600"
     CDS             complement(2169..2765)
                     /locus_tag="VNN25_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91034.1"
                     /translation="MELIAQQYRDAIAALQTIMPLLKFDSRQTRRVAASAKFAAGLIE
                     PTIAMVTSASLMKDRNLFNVEEAEFALELRSQIAPLFRQLASLAADGLFTIDSKLSLS
                     SVKALQAYKLVQHAAAQPDGAELDPYLAEMERAVMKALNRSKRSAPVPTPEPTPTPAE
                     LPPGARGFMAPRPAAAKTAEPESVADLFDKALADVTKK"
BASE COUNT          703 a         1065 c         1087 g          734 t
ORIGIN      
        1 cagctatctc attcaacgaa cggacggcaa cgtcctgatc gactcaccac gcttctctgg
       61 gccgctcgtg aagaacatcg actccatggg cggcgtgtca aagttgctgc tgacgcatca
      121 ggacgacgtc gccgatcacg cgaagtttca cgatcgcttc ggctgcgagc gcgttctgca
      181 tcgcgacgac gtgcattcgc ggacggcatc gatcgagctg cagccggccg gcatcgatcc
      241 aatctcgctc ggcaacgaaa tcgtgatgat cccgactccc ggccacacga aaggccacgc
      301 ggtgtttctc tatcgcgatc gctatctctt caccggcgat catctcgcct ggagcgagcg
      361 ccgcggccac ctctacgcat tccgcagcgc ctgctggtat tcgtggagcg agcagatccg
      421 ctcgatgaaa cgattgctcg actatcgctt cgagtgggtc ctccccggcc acgggcgccc
      481 agtccacctt gatccggctg cgatgcgcgc gagtctggag cgatgcgtcg tctggatgaa
      541 agccaattga caattgatca tggacaattg acaacgcgcg gtgcgtgtcg gccagcgttg
      601 tccattgtca attggtaggt acagccattg ctctacgcgt gagggatgaa gtatttcgcc
      661 ctcctcaagc aaaccatctc cgaattcggc aacgacaagg cgccccgcct cggcgcagca
      721 ctggcgtact acacaatctt ctcgctcgcg ccgctgctgc tcatcgtcat cgcaatatcg
      781 ggcctcgcct tcggccatga cgccgcgcag aacaagatct tcgagcaatt gcgaggagtt
      841 ctcggtccgc aggctgccgt cagcgttcag gacatggtca agaacgccgc gaagccgaag
      901 agcggaacgg tcgccacgat catcggcatc gtcacgctga tcttcggcgc ttcgggcgtc
      961 ttcggccagc tcaaggacgc gctcaacacg atctgggacg tcaaaccgag agagggcggc
     1021 ggaatcctga cgctggtcaa ggaccgtttc ctctcgttcg cgatggtcct cggcgtcggc
     1081 ttcctcctgc tcgtttcgtt ggtgatcgac acggcgatca cggcaatcgg aacctacgcc
     1141 ggcagtcacc tcccgggcgg tgaagtgctc tggcacgcgg cagagctcat cttttcgttc
     1201 tgcgtgatca cggtgctcat ggcgatgatg ttccgcctcc tgcccgattt gaaaatcgaa
     1261 tggcgcgacg tctggctcgg cgccgcaatc acctcgctac tcttcgtcct cggcaagttc
     1321 gcgctcggca tctacttcgc gaaaagcgcg gttggatcga gcttcggcgc cgccggttcc
     1381 ctcgtcatcg ttctcttatg ggtttattac tcagcgcaga tcctgttgtt cggcgcggag
     1441 ttcacgcagg tctacgccca cgcacacgga agtttgaagg gcgcaaagca gtcagaagca
     1501 ccgcagcccg caccaatacc ggtcgtcgta ctcaaaccct cggcagcggg cggggcagcc
     1561 aagctgctca ctggcggcgc tgcaggcctc atcatcggac tgattgccgg gttagtcggc
     1621 gcaatcgtcg tgtttgtgaa gtcagcgaag aaactggtta cttgattcga gttacttgct
     1681 tgacgccgct ttcgccacca gtccgattcc gatcgcgatt gccgcagcgc cggcggccac
     1741 ccacgaagca ttcgaccgcg cgttcttcag gcccttcctg accgcgcgta ccgcgtcatg
     1801 cctccgcttg taagtctcgc cgtcgagctg catgacggtg cctttcacac tcggcgaatc
     1861 gagaattctg ccaatggccc agaccgccag cggaggaatg agccgcgtca tcgtcgtacg
     1921 ggtcggagat ttgacgttca tgagattacc cctgagcgtt gaaggagcaa ggaccctgcc
     1981 gggttgggtg gcgcgggttc cgaggttggg cagtctcgtg gacgccggga cactccaagg
     2041 cccgcgaggc tccgcacaag cgatctccgt tcccgaggtg gcaatctccg ttggcgagat
     2101 ggtgatttct cctaaccctg aaaccgccgc cccccacccg gccaaatggg gggcggcgca
     2161 tatgggggct acttcttcgt cacgtcggcg agggctttgt cgaagaggtc ggcgacgctt
     2221 tccggctcgg cggtcttggc tgcggccgga cggggcgcca tgaaacccct cgcgccgggc
     2281 ggcagttcgg ccggggtcgg ggttggctcc ggggtgggaa ccggggcgga gcgcttgcta
     2341 cggttcagcg ccttcatcac agcacgctcc atctcggcaa ggtaggggtc aagctcggca
     2401 ccgtcgggtt gggcggcggc gtgctgcacc aacttgtacg cctggagtgc ttttaccgag
     2461 gagagagaca gcttgctgtc gatcgtgaac agcccgtcgg ccgcaagcga ggccagttgc
     2521 cgaaacagcg gggcgatctg actgcgcaac tccaacgcga actcggcttc ctccacgttg
     2581 aacaggttcc gatccttcat cagcgaagca gaggtgacca ttgcgatcgt cggctcgatc
     2641 aagccggccg cgaacttcgc gctggcggcg acacgcctgg tctggcggga gtcgaacttc
     2701 agcagaggca tgatcgtctg tagcgccgcg atggcatcgc gatactgctg ggcgatcagc
     2761 tccactgcct tcgcggccgc cgccgcgggg ccgtcagggg tggttggtgt ggatagggag
     2821 ggggaagtat cagtcacaga catcgtctaa ctccttacta cagcgtcatt cgtcaatacc
     2881 cggccgggcc tgtctactca cccggcaacg gacgcgttgc cgggcggtct gtttttttct
     2941 cagacatagg tttgacgcac gagctcggct gaggtttctg gagctgaggt tacggagggc
     3001 cgaaaggtat cttccgaggg gggcaaaaag cgggatttcg gagcaatcca aatttgctcc
     3061 ggagcaatcc aaagttgctc cgtaggaaag aaaagatgct ccgtaggaaa aaaaagattc
     3121 tccggaggaa agaaaagttg ctacggagca tcaaaagatt ctccgtacgg gggagaaagt
     3181 ttctacggag catcagaaag attctccgta gggggaaaaa gatgctacgt aggaatccaa
     3241 agatgctact taggaaagaa aagatcctgg ggagcaataa aaagtttcta cgtaggaaag
     3301 aaaagatcct ccgtaggaaa gaaaagatgc tccgtaggaa tccaaagatg ctccgtagca
     3361 aagagaagat gctcgaaatc ggccgttccg agctgcgaag ggtcaggcct ttgctttcct
     3421 ggaagttgta actcgttggc agtcagttac ttgtgcgccg gagcgctggc gtctcgctgg
     3481 ctgggtcaat ttgaagggag tttgtaacgt gacccctcgg cccttccttc cgggcagtgt
     3541 ccactgtcca attcgcccgc cctttcggct gccagtttag ccattccaa
//
LOCUS       DATLVW010000174         8101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_213436, whole genome shotgun sequence.
ACCESSION   DATLVW010000174 DATLVW010000000
VERSION     DATLVW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213436"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1719)
                     /locus_tag="VNN25_05605"
     CDS             complement(274..1719)
                     /locus_tag="VNN25_05605"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXH91035.1"
                     /translation="MTTFVLIGAIAVLLVLGVLVLVLGNRHARRYQHSRRIRVDRFKL
                     KRRHAAIELEVFGSREVVDAVRVYAKDHHVSIEEATHQARVYLREIVPKFNLLAYYRL
                     GAPLARGIMHFLYRPVVEHKTLRDFNARAPKNAVVVYLINHRSNADYVLVAHMLFKFV
                     SLSYAVGEWARVWPLNHLFKWFGGYFIRRRYREPLYHAILSSFVSTITKNGVTQGVFI
                     EGGLTRDGAFQKPKLGMLDYIVGGKRDPGFTAPLFLIPTAINYDRVLEDRNLTEELIG
                     KEDRRSKSEKLKTTGDFLFRSLLRSIFKRFKRYGYAVVTFGAPVSVDDFIREHPSILS
                     SSFDDRKEAMTGLAELVMTEISNALPVTPVTLLARIFADRPDTPLTDEEIVDAIDGYR
                     TTWQDRVWLLREKTGTEIWHAARRVVELRHLIQPAERWRSDLFEGSGVPVIEEAWQWN
                     PRETLLRDYYANSLMTFAEVKTRGWPERRKA"
     gene            complement(1862..3928)
                     /locus_tag="VNN25_05610"
     CDS             complement(1862..3928)
                     /locus_tag="VNN25_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HXH91036.1"
                     /translation="MSQVIRAVCPHDCPDTCMMLVTVDDDGRATRIGGDPEHAFTQGF
                     LCTKVSKYLERTYHSDRLTFPQIRAGAKGEGRFRRATWDEALALIAERLSAIIASPDG
                     PQAILPYSYAGTMGLVQGEAMASRFFRRIGASFLDRTICSSAGGEAFALTYGAKMGTD
                     PEAVPEAKLILLWGTNTLTSNPHLWPFIRKARANGAPVICIDPIRTRTAAASDEHIAI
                     RPGTDAAVAMGMMHVLFRDGHVDRQYLDEMTHGWEKLRDRVLAEYAPERVAAICRLPV
                     ETIERLGTLYGTTRPTFIRLNYGLQRHAGGGSAVRAISLLPAITGAWNDAGGGCQLSS
                     SGTFAMNTAAMERTDLGNHAARTINMTLLGRALTEVDNPPVKGLIVYNSNPGSIAPDR
                     ENVLRGLRRDDLFTVVLEHFQTDTADYADVLLPATTQLEHEDIHKAYGHLYAMYNRRA
                     IEPLGEALPNSEIFRRIAAAMKLDFPELRESDEELMRSALTDTAEVMGGVTLEALREH
                     GSVRLNLPTPHMPFRRGAMLKTPSGKIEIDSAQVAALGLDPVPDYIAPRESEERAPEL
                     ARRFPLALLSPPAHEFLNSSFANVPSLRRSAGKPALEIHADDAKARAIEDGARVRVFN
                     DRGTFTADAVVTDRVCPGVVSAPSVWWGKLSGDGANANQTTSQALTDIGRGATFYDNL
                     VEVAPV"
     gene            complement(4119..4472)
                     /locus_tag="VNN25_05615"
     CDS             complement(4119..4472)
                     /locus_tag="VNN25_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXH91037.1"
                     /translation="MAKSKGGKIESKLRDYALAFPEAYEEFPWGHSAIKVKKKAFLFM
                     GTGEDGDFSISVKLPHSRDMAVDLPFTEPTGYGLGKSGWVTAHLKDEKSLNLIKVWIE
                     ESYRANAPKKLVKGL"
     gene            complement(4475..4849)
                     /locus_tag="VNN25_05620"
     CDS             complement(4475..4849)
                     /locus_tag="VNN25_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXH91038.1"
                     /translation="MRTNPLRKVSDICLAFPEATVERHGSHATFRVRKKVFAYFLDNH
                     HGDGIVSVCVKTRLGENQDLARDQPSRFYLPAYIGPRGWVGVRLDFPKVSWNEVAAFI
                     ASSFAIVAPKTLAAAARRNVNS"
     gene            complement(4862..5242)
                     /locus_tag="VNN25_05625"
     CDS             complement(4862..5242)
                     /locus_tag="VNN25_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH91039.1"
                     /translation="MAGERDLRPPVWIGHVVLETDRLAETEQFMRTIGMRSVVQRPEV
                     AVLELRGGTHLVLTPRTGAVPGDAPFDLMVEDLEATHQRFVDLGLDPSPIGTASANHR
                     TFSVREPAGNVITFFSNHVSGNPV"
     gene            complement(5247..6539)
                     /gene="fadI"
                     /locus_tag="VNN25_05630"
     CDS             complement(5247..6539)
                     /gene="fadI"
                     /locus_tag="VNN25_05630"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HXH91040.1"
                     /translation="MWQYTPRVAVVRGLRTPFAKSGTHYARLSALDLGKLVVAELVQR
                     SGIDPNSIQELVFGNVIPSVKAPNIAREIVLGTGLPRTLPGYTVSKACASSNQAITSA
                     ADLIFRGYADVVVAGGSESLSDIPILFSKNFSDALVGASKQKSIGRKLGSFSKIRPKD
                     LAPDAPAIAESTTGLTMGESAEKMAKENGITREAQDKFALQSHHRAAEATASGRFKDE
                     VMTVMVPPGFDNIVETDNLIRGDSTAEAMAKLRPVFDRKYGTITAANSSPLTDGASAV
                     LLMSEDKAKALGIKPIGFIKSYAFAATDPFDQLLQGPVFALPIALERAKITLKDIGII
                     EMHEAFAAQVLSNIQWIGSKKIAQEKLGRSEPVGEIDPEKINLTGGSIALGHPFGATG
                     ARIVTTVCNELQRTGQQYGLVTVCAAGGIGVAMVLERE"
     gene            complement(6605..>8101)
                     /locus_tag="VNN25_05635"
     CDS             complement(6605..>8101)
                     /locus_tag="VNN25_05635"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXH91041.1"
                     /translation="GLTAATSVRYTVNNIDANDGQFVTTPSCSGPPGSSTLFGITSCG
                     DGAASVLLSWTAASGALQYRVNRDGAVIGTTTTDRTFTDDAVIAGHSYSYTISALNSS
                     GSTESNTLTATIAPCVPPPGAFAASASTFCTTGASPAPAVHVSWTASSNAASYVVNRN
                     GAAYTSSLSSGTLALDDLNVTVGQTYAYTVTATNSGGSTVSSGKSVTITSAICPATRP
                     TPPVLSENTVCNGTSPGDHLSWTASANATSYIVFRNGTALQSLPSSTLAYDDTSVIPG
                     QTYSYFVRASNSGGTADSNTINISLPTTICQPPPAPFALTATAICDIAASPSAAIRLT
                     WTASANATSYAVFRAGTKIADVSTTSFVDTANIVAGQSYAYLVRASGPGGTTDSEEFD
                     LTVDPAVCQSACSFACAANVVATAAEEFPVRFSLQQQTSCADVTATWAFGDGTGSTEL
                     TPTHTYPSPGIYRWTVTAGSAASAACQNSGTITITSVPPPAKRRAIRH"
BASE COUNT         1502 a         2417 c         2679 g         1503 t
ORIGIN      
        1 atatcaaaca accgctttcg atatcgagac tggagactcg aatatcaggg aagtctccgc
       61 atccgcagat gacgcagatc gacgcagatt caggacgctc atctgcgttt catctgcgtc
      121 atctgcggat aagcaagaga agcgcgctat gcggcgtcga cgtgggcgca gcggcgttgc
      181 gcccacctgc caacggaaac tgcgtgtggc cgttccattc gagaaccacc gccgggcgag
      241 gacgcccggc ggttgcaggc aagaatgcct gcactacgcc ttcctccgct ccggccaccc
      301 gcgcgtcttc acttccgcga acgtcatcag cgagttggcg tagtagtcgc ggaggagcgt
      361 ttcgcgcggg ttccactgcc atgcttcctc gatgacggga acgccgctgc cttcgaagag
      421 gtcggagcgc cagcgttcgg cgggctggat caggtggcgg agctcgacga cgcggcgggc
      481 ggcgtgccag atttcggtgc cggtcttttc gcgcaggagc cagacgcggt cttgccatgt
      541 ggtgcgatag ccgtcgatgg catcgacgat ctcttcgtcg gtcagaggag tgtcgggacg
      601 gtcggcgaag atgcgcgcga gcagcgttac cggcgtgacc ggcaaggcgt tcgagatctc
      661 ggtcatcacc agttcggcga gaccggtcat cgcttccttg cggtcatcga acgacgacga
      721 aaggatcgaa ggatgttcgc ggatgaagtc gtcgaccgag accggcgcac cgaacgtgac
      781 cacggcgtag ccgtagcgtt tgaaccgttt gaagatgctg cgcagaagac tgcggaagag
      841 aaaatcgccc gtagtcttga gcttctccga cttggaacgc cggtcttcct tgccgatcag
      901 ctcttcggtg agattgcgat cctcgagcac gcggtcgtag ttgatcgccg ttgggatcag
      961 aaagagcggc gcggtgaatc ctggatcgcg cttgccgccg acgatgtagt cgagcatgcc
     1021 aagcttcggt ttctggaacg cgccatcgcg cgtcagtccg ccttcgatga agacgccctg
     1081 cgtcacgccg ttcttcgtga tcgtgctgac gaagctggag aggatggcgt ggtagagcgg
     1141 ctcgcgataa cggcggcgga tgaagtagcc gccgaaccat ttgaagagat ggttgagtgg
     1201 ccagacgcgc gcccactccc ctaccgcgta cgacagcgag acgaacttga agagcatgtg
     1261 cgcgacgaga acgtagtcgg cgttggagcg gtgattgatg aggtaaacca ccaccgcgtt
     1321 cttcggcgca cgggcgttga aatcgcgcag ggtcttgtgc tcgacgacag gtcgataaag
     1381 aaagtgcatg atgccgcggg cgagaggcgc gccgaggcgg tagtaggcca ggagattgaa
     1441 cttggggacg atctcgcgga gatagacgcg cgcctggtgc gtggcctcct cgatcgagac
     1501 gtgatgatcc ttcgcgtaga cgcggacggc atcgacaacc tcgcggctgc cgaacacttc
     1561 gagctcgatc gccgcatgcc gccgcttcag cttgaagcgg tcgacgcgga tgcgccgcga
     1621 gtgctggtaa cggcgcgcat gacgattgcc gaggacaagg acgagcacgc cgagcacgag
     1681 cagcacggcg attgccccga tcagaacaaa ggtcgtcatg ggatagcgcg actttatcgc
     1741 gcttgcgtca aaaggcagag atcagagact ggagatcaga gactagagtc gttaataact
     1801 gcccgatctc tgatctcttg tctctcatcg cctcctgcca atcgcccgct tggagcccag
     1861 atcagaccgg cgcgacctcg accaggttgt cgtaaaacgt cgcaccgcgg ccgatgtcgg
     1921 tcagcgcctg tgacgtggtc tggttcgcgt tggcgccatc gcccgaaagc tttccccacc
     1981 acaccgacgg cgcggagacg acgcccggac aaacgcgatc ggtgacgacg gcgtcggcgg
     2041 tgaaggtgcc gcgatcgttg aagaccctga cgcgagcacc gtcctcgatg gcccgtgctt
     2101 tggcgtcgtc ggcatgaatc tccagcgccg gcttgcccgc ggaacgacgc agcgatggaa
     2161 cgttggcgaa gctggagttg agaaactcat gcgcgggagg cgagaggagc gccaacggaa
     2221 aacggcgcgc cagctcggga gcgcgctcct ccgattcgcg cggcgcgatg taatccggaa
     2281 ccggatcgag gccgagcgcg gcaacctgcg ccgagtcgat ctcgatcttc ccgctcggcg
     2341 tcttcagcat tgccccgcgc cggaacggca tgtgcggcgt cggtagattg agccgcacgg
     2401 agccgtgctc acgtagcgct tccagcgtca cgccgcccat cacttcggcg gtgtcggtga
     2461 gcgccgaacg catcagctct tcatcgcttt cgcggagctc cggaaagtcg agcttcatcg
     2521 ccgcggcgat gcggcggaaa atctcggagt tcggtaacgc ctcgcccaac ggctcgatcg
     2581 cacggcggtt gtacatcgca tagagatgac cgtaagcctt gtgaatgtct tcgtgctcga
     2641 gctgggtggt ggcaggaaga aggacgtcgg cgtagtcggc ggtgtcggtt tggaagtgtt
     2701 cgaggacgac ggtgaagaga tcgtcgcgcc gcaaaccacg cagcacgttc tcacggtcag
     2761 gtgcgatcga tccgggattg gagttgtaga caatcaaccc tttcaccggc ggattatcca
     2821 cctcggtcag cgcgcggccg agcagcgtca tgttgatcgt gcgcgcggca tggttgccga
     2881 ggtccgtgcg ctccatcgca gcggtgttca tggcgaaggt gccggatgac gagagctggc
     2941 aaccgccgcc ggcgtcgttc catgcgccgg tgatcgcggg cagcagcgag atggcccgca
     3001 cggcggaacc accgccggcg tgacgctgca gtccgtagtt gaggcggatg aaggtgggac
     3061 gcgtcgttcc gtagagagtg ccgaggcgtt cgatggtctc gacggggagc cggcagatgg
     3121 cagcgacacg ttcgggtgcg tactcggcga gaacgcggtc gcgaagcttt tcccaaccgt
     3181 gcgtcatctc gtcaagatat tgacgatcca catgtccatc tctgaacagg acgtgcatca
     3241 tccccatcgc caccgcggcg tcggttccgg ggcggatcgc gatgtgctcg tcggacgcgg
     3301 ccgccgtcct cgtgcgaatc ggatcgatgc agatgaccgg cgcgccgttc gcgcgcgctt
     3361 tccgaatgaa gggccagagg tgaggattcg acgtgagcgt gttcgtgccc cagagcagga
     3421 tcagcttcgc ttccggaacg gcttccgggt cggtgcccat cttcgcgccg taggtcaaag
     3481 cgaatgcctc cccacctgcg ctcgagcaaa tggtccggtc gaggaacgac gcgccgatgc
     3541 ggcggaagaa gcgcgaggcc atcgcttcgc cctgcacgag gcccatcgtc ccggcgtacg
     3601 agtacggcag gatcgcctgc ggaccgtcgg gcgaggcgat gatggcgctg agccgctcgg
     3661 caatcagcgc cagcgcttcg tcccacgtgg cgcggcggaa gcgtccctcc cctttcgcgc
     3721 cggcgcgaat ctgcggaaag gtcagccgat ccgagtggta cgtccgctcc agatacttcg
     3781 acactttcgt gcagaggaaa ccctgcgtga acgcgtgctc gggatcgccc ccgatgcgcg
     3841 tggcccggcc gtcgtcatcg accgtgacga gcatcatgca ggtatcggga caatcgtgcg
     3901 ggcagaccgc gcggataact tgggacatgg ttgagtaagt ttaacccggc gcgaaagacg
     3961 atgaaatcgt cctcgtgatg caccatgaaa atcgaacgct gaccgttcga ttttcagggt
     4021 cgctgcgcat tgcgattcat cgtgcgagac ctccgctctt acgcgacacc accgtcgggc
     4081 ggggacgccc gacggccgcg cgcgagacgc ccgcactcct agagtccctt caccagcttc
     4141 ttcggcgcat tcgcacggta actctcttcg atccatactt tgatcaggtt gagcgacttc
     4201 tcgtccttca agtgcgcggt gacccagccg ctcttgccga gaccgtagcc ggttggctcg
     4261 gtgaagggaa gatcaacagc catgtcgcgc gagtgcggga gcttgaccga gatcgagaag
     4321 tcgccgtctt cgccggttcc catgaagagg aaagccttct tcttcacttt gatcgcactg
     4381 tgtccccacg gaaactcttc gtaggcttcg gggaacgcga gagcgtagtc gcgaagcttc
     4441 gactcgatct tgccgccttt ggatttcgcc atggtcagga gtttacgttc cggcgagccg
     4501 ctgccgccag agtcttcggg gcgacgatgg cgaagctcga cgcgataaac gccgcgacct
     4561 cgttccacga aaccttgggg aaatcgagcc gcactcctac ccaaccccga ggaccgatgt
     4621 atgccggcag atagaaccgc gacggttgat ccctggcgag atcctggttc tcgccgaggc
     4681 gcgtcttcac gcataccgat acgatgccgt caccatgatg gttgtcgagg aagtaggcga
     4741 agaccttctt gcgaacgcgg aacgtagcgt gcgagccgtg ccgctcgacg gtggcttcgg
     4801 ggaaggcaag gcagatgtcc gagaccttgc gaagaggatt ggttcgcatc gcggtcagcg
     4861 ctcacactgg attgccggaa acatggttgg agaagaacgt aatgacgttt cccgctggtt
     4921 cccgaactga gaacgtcctg tggttggccg aggcggttcc gatcggcgag ggatcaaggc
     4981 cgagatcgac gaaacgctgg tgcgtggctt cgagatcctc gaccatcaga tcaaatggtg
     5041 catcgccggg aaccgctccc gtcctcggcg tgagaaccag atgcgtaccg ccgcgaagct
     5101 ccagcacggc cacttccggc cgttgaacga cagagcgcat cccgatcgtg cgcataaact
     5161 gctctgtctc tgccaggcgg tccgtctcca gaacaacgtg gccgatccag acgggagggc
     5221 gcagatctcg ttcgccggcc atcggcctac tcccgttcca acaccatcgc cacgccgatc
     5281 ccgcccgccg cgcaaaccgt cacgaggccg tattgctggc cggtgcgctg cagctcgttg
     5341 cagaccgtcg tcacgatgcg ggcgccggtc gcgccgaagg ggtggcccaa ggcgatcgat
     5401 ccgccggtga gattgatctt ctccggatcg atctcgccga ccggctcgct gcggccgagt
     5461 ttctcctgcg cgatcttctt cgatccgatc cactggatgt tcgacagcac ctgcgcggca
     5521 aacgcctcgt gcatctcgat gatgccgatg tccttgagcg tgatcttcgc gcgctcgagt
     5581 gcgatcggaa gcgcgaagac aggtccctgc agaagctgat cgaacggatc ggtagccgcg
     5641 aacgcgtagc tcttgatgaa gccgattggt ttgatgccga gcgcttttgc tttgtcttcc
     5701 gacatcagca ggaccgctga cgcgccgtca gtgagtggcg acgaattcgc agcggtgatc
     5761 gtgccgtact tgcgatcgaa caccggccgc agtttcgcca tcgcctcggc cgtcgaatcg
     5821 ccgcgaatca ggttgtcggt ttcgacaatg ttgtcgaatc ccggaggcac catcaccgtc
     5881 atcacttcgt ctttgaatcg cccgctcgcc gtcgcctcgg cggcgcgatg atggctctgc
     5941 agcgcaaatt tgtcctgcgc ctcgcgcgtg atgccgttct ctttggccat cttctcggcc
     6001 gactcgccca tcgtcagtcc ggtcgtcgac tcggcgatgg caggcgcgtc gggcgcgaga
     6061 tccttcggac ggatcttgct gaacgatccc agtttcctgc cgatgctttt ctgtttgctc
     6121 gcgccgacga gggcgtcgga gaagttcttc gagaagagga tcgggatgtc ggagagcgac
     6181 tcgctgccgc cggcgacgac gacatcggcg tagccgcgga agatcagatc agcagccgag
     6241 gtgatggcct gattcgacga cgcacacgcc ttgctgaccg tgtagcccgg caacgttcgc
     6301 ggtaggccgg tgccgaggac gatctcgcgt gcgatgttcg gcgctttgac cgaggggatg
     6361 acgttgccga agaccaattc ctggatgctg ttgggatcga tgcccgagcg ctgcacgagc
     6421 tccgcgacga cgagcttgcc gaggtcgagc gcggagaggc gcgcgtagtg ggttcccgac
     6481 tttgcgaaag gtgtgcgaag acctcgaacg acagcgacgc gaggggtgta ttgccacatg
     6541 cggcgaagat tagcacgcgc gaacgccgcg gcccttcggc cgattcagtg ggtggcggcg
     6601 aggttcagtg ccgaattgcg cgccgttttg cgggcggcgg gacggaggtg atcgtgatcg
     6661 ttccgctgtt ctgacacgcc gccgatgccg cactcccggc tgtgacggtc catcggtaga
     6721 tcccggggct cgggtacgta tgggtgggcg tcagctcagt cgagcccgtg ccgtctccga
     6781 atgcccacgt cgcggtcaca tcagcgcagg aggtctgctg ctgcaacgaa aaccgtacgg
     6841 ggaactcctc cgcggcggtt gcgaccacat tggcggcgca cgcgaagcta caggcagact
     6901 ggcagaccgc gggatcgacg gtcaaatcaa actcctccga atccgtcgta cctcccggac
     6961 cggatgctcg gacgaggtac gcatagctct gcccggcgac gatgttggcg gtgtcgacga
     7021 atgatgttgt ggatacgtca gcgatcttcg ttccggcgcg gaagacagcg taggaggttg
     7081 cgttcgctga cgccgtccaa gtcaaacgga tggcagcgga aggcgatgcg gcgatgtcgc
     7141 agatcgccgt cgcggtcagc gcgaatggtg ccggcggcgg ctgacagatg gttgtcggca
     7201 gcgagatgtt gatcgtgttc gagtcagccg tgccgccgct gttcgaagcg cgaacgaagt
     7261 acgagtacgt ctgacccggg atgaccgagg tgtcgtcgta cgcgagggtc gatgagggca
     7321 atgactgcaa cgccgttcca ttgcggaaga cgatgtaact cgtagcgttc gcagacgcgg
     7381 tccacgagag atggtcgccg gggctggtgc cattgcaaac tgtgttctcg gacagcaccg
     7441 gcggggtcgg ccgggttgcc ggacagatcg cgctcgtgat cgtgacgctc tttccgctcg
     7501 acaccgtgct gccgccgctg ttcgttgccg tcaccgtgta agcgtaagtc tgtcccacgg
     7561 tcacgttgag atcatcgagc gccagcgtgc cggaggagag cgaagaggta taggccgcgc
     7621 cattgcgatt gacgacgtag gaagcggcat tcgaggaagc ggtccacgag acatgcaccg
     7681 ctggcgcagg cgatgcgccg gtcgtgcaaa acgttgaagc agaagccgcg aatgcgccgg
     7741 gcggagggac gcaaggggcg atggtagccg tgagagtgtt cgactcggtc gaaccggagc
     7801 tgttcaacgc ggagatcgtg tagctgtacg aatgtccagc gatgacggca tcgtccgtga
     7861 acgtacgatc cgtcgtggtc gtaccgatta cggcgccatc ccgattgacg cgatactgca
     7921 aggctcccga cgcggcggtc cagctcaaca gaacgctcgc ggcgccatcc ccgcacgagg
     7981 tgatgccaaa aagggtagag cttccgggcg gaccggagca tgacggcgtc gtcacgaact
     8041 ggccgtcatt cgcgtcgatg ttgttgaccg tataccgcac ggaggtggcg gctgtaagcc
     8101 c
//
LOCUS       DATLVW010000175         9267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_418750, whole genome shotgun sequence.
ACCESSION   DATLVW010000175 DATLVW010000000
VERSION     DATLVW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418750"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1419
                     /locus_tag="VNN25_05640"
     CDS             142..1419
                     /locus_tag="VNN25_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXH91042.1"
                     /translation="MRHAAFPDGLSRDRLIEWYRETRKVTRALFTIPTEEAYYDRPIR
                     LRNPIVFYEGHLPAFAVNTLIKLALKREGIDSELETLFARGIDPENEEAAKTPTDFWP
                     TRDEVQRYGGNAEDQIIRALCDGPIDDGLVPQRRNAEAAFTILEHELMHQETLLYIFH
                     ELPYERKNALAPLRSGRTATRSTPVTKDMVRIPAGEATLGATGTDFGWDNEISQLRVA
                     VGDFAIDRHNVTNGDYLEFVRATGAEGPHFWLKRGDEWYWRAMFDLTPLPLDCPVYVT
                     HEEASAYAAWRGKRLPSEGEYHRAAFGTPDGDERSFPWGDALPDTSRGNFGYANWDTV
                     PVGSYPDGVSAWGVHDLVGNGWEWTSTLFEPFPGFEPMSSYPVYSADFFDGKHYVLKG
                     ASPATAPELIRRSFRNWYRPAYPYVYATFRCCA"
     gene            1525..2130
                     /locus_tag="VNN25_05645"
     CDS             1525..2130
                     /locus_tag="VNN25_05645"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXH91043.1"
                     /translation="MTLTQTYKPKTFNLSGLTGISDKTLEMHFKLYEGYVTNTNLLSE
                     KIASILEDGSVDKEEMPAYSELTRRLGFEYNGMVLHEYYFGNMKRNAGGNVPRGSKFR
                     AAIERSFPSFEIWKTDFVSVGKMRGVGWAIAYLDPTAGRVSNHWIELHENGNVAGFIP
                     LLVMDVWEHAYLLDYKPADRPKYIEAFLANVDWDAVEKRMA"
     gene            complement(2134..5667)
                     /locus_tag="VNN25_05650"
     CDS             complement(2134..5667)
                     /locus_tag="VNN25_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH91044.1"
                     /translation="MHDHAVFFDPSHRRWWWIKRIGTLLGLMAVVTVSFWLVSLFTVP
                     LLPGFRGITEDIRRGLRVPAHKQARAQYLQKNARERLLSLVRQDRKNQLAREAKGPIR
                     AIETGKPIVAAFYAPWQETGLHSLDANASRMTHLLPVWVHLRADAQGLDFHDWDPLLT
                     PHNLEVLKKARANNLNIVPVFSNAQVSTNGSGEFDPKRVHLFLNNPAIQAKTILDLKR
                     WCVTMRFQGINVDFENLDAADYPLLIPFLKRMKTSFAQKGLLVSIDLEARKEAREKLD
                     WRAVASICDFVVVMAYDEHSENSPPGPIASISWYRGVVLNAVQSIPREKLVIGIANYS
                     YDWMEGREWAEPLTYQGALVMAHDYRQEEKPEDIVDFDETALNPTFRYKDDDGKEHEV
                     WMLDAVTAANQWLIGQNYGVRGLAIWVLGSTDPSIWSFIDRDHLNDPVNMKALSEPDF
                     PYDVEFVGEGEIAHVDKLPTKGSRSLEIDPQTGLALDEDYHKFPTSYVISRTGYKPKM
                     IALTIDDGPSSPYTSDILDELKALHVPATFFLIGQNAERYPNLVRRIWNEGHEIGNHS
                     YTHPNIGVIPPQEARLELNATQRVFQSLLHRSTLLFRPPYNADAEPTSAMEVIPIKMA
                     SELNYITVLEFIDTQDWNTEEKMPNGSIHRRNANDMWETAWAQLDGGEHGSCILLHDG
                     GGDRTETVKLIPILVRELHKRGYTFVPVSTLIDSTRDKVNPPVNPGDTLMAANDRIVF
                     EVIYLFELFLGVAFITAIILGTARIVFVTILALVAKWRERHRRFDDAVRPTISVVIAA
                     FNEATVIARTIRAVLENNYAPLEIIVVDDGSADGTADEVRANFGDSVTLLVQPNGGKA
                     SALNLGIAMATGEIIIALDADTLFARDTIEKLVRHFADPLVGAVAGNVKVGNRMNPLT
                     HWQSIEYVTSQNLDRRAYAIINSVTVVPGAVGAWRREAILQAGGYSTDTMAEDMDLTW
                     RIRRIGWRIETESAAVGYTEAPDSFRALFKQRFRWAFGTLQSLWKHRRAVGRYGWFGR
                     VMLPALWLFQVAFQVLSPLIDLQILWSIASVVRAWMRGRLAGDWQPLPGALSSLYLIG
                     FMYAFFFVMELIGSLIAYKLDRENPKVLVWLFWQRFLYRQLMYAVVLRSLKTAASGMR
                     TGWGKLERKGTVEMDGQVIAEPAELQVREAQ"
     gene            5755..6723
                     /locus_tag="VNN25_05655"
     CDS             5755..6723
                     /locus_tag="VNN25_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91045.1"
                     /translation="MTQTVIEVSGLKKQYRSAVGRSRITALDGIDFTVREGELFGLLG
                     PNGAGKTTAVKILLDLTRPTAGEARINGLPAGNPESRRRVGYLPEGHKIPGYLTARQA
                     LGIFGKMSGLDDLTIKRRSVELLEKLRIAQWIDVRVKKYSKGMTQRLGLAIALLHSPH
                     VLLLDEPTDGVDPVGRREIRDILQAEAAANGTAVLLNSHLLSEIELTCNHVAVLRNGK
                     VAAAGSIEELTRRAAKYKMVPATPVDDALVASFRESGAGVERVNGHMLLTVDDVTHLN
                     ALVDKLRAGGGLLTELSPVRSTLEDVFVDLVRAVDPGAQQSEGALL"
     gene            6720..7568
                     /locus_tag="VNN25_05660"
     CDS             6720..7568
                     /locus_tag="VNN25_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXH91046.1"
                     /translation="MKVLMANIEDVMREAAARWTLVAYFFLSTIFIIIFASAINLDIV
                     DGALAGAKLFGKQVEMNSSISIEKLVMGFESGFSGVLYVLCTFLAIFATAHLVPRMQE
                     KGTIDLYLSRPVSRVKLILSRYVAGLILAGSNVLYLIGSIWMIVAWKTHVFHPRFFLA
                     GVVILFLIATLLAFAFLIGVITSSTAVSIMATYGVFFFGVMLAGHARIEAALSKEWQA
                     FAIKMLYWIMPKTAELATAVVAYVAGGEIHNEMLTITPAPFVSTAVFGLVCLVLASWL
                     FQRKEF"
     gene            7569..8549
                     /locus_tag="VNN25_05665"
     CDS             7569..8549
                     /locus_tag="VNN25_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91047.1"
                     /translation="MKKQLVSLAVLLLALAPAAFAKNDAMSLIPNDSVSVGVVRLNDM
                     RSSSLSAALFGQTAKISNDGEAQKFLSESGLQPAKDVDVIVFATSPRTNLGSEADFLI
                     AADGRFNVERLTAALVTRGAVKKTSPHGVYYLLPEKGTEPHHQGVVAFPDAHLALIGS
                     EAAVVGALANHAAGGTSFATASGLGRDMVRIDPSATAFVLVDVPRAQRITGAPKLNGN
                     SSSSQALGAALKNVSTVAIWATDTGDSLKLNAIGLSTDAETLGLVEDTLRGALSAMRL
                     AVQDKSPEMVSTLRKFTVSRTDNSVSISGSISGEAFRTWVAKSQAHGSMR"
     gene            complement(8615..>9267)
                     /locus_tag="VNN25_05670"
     CDS             complement(8615..>9267)
                     /locus_tag="VNN25_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91048.1"
                     /translation="DIVGPLGESRVTDRTGTTTAFFDANGNRILSSGYSTSIDLTTPP
                     GRYRIEVANLGTLAPGVNKTTTATMNVDSSQPDYFPPVVTTMMLLDGGGAVVTRLEPH
                     GSGSLLFSAADYIGSTSISGRVYQQVRSDATKVSYRYGGETAWRPLTATQVTEDTAHG
                     AGILYRVDLGAITNVDRSLVDLKFDLADVTGNTTTVTMVPAFSVGPELPPRRRTAH"
BASE COUNT         1769 a         2800 c         2870 g         1828 t
ORIGIN      
        1 gggcgcacga aaggcaacat ccctgtctac aacgtggcga tgccgctact gatcgccaac
       61 gatggaagca ggccgccttt ccgcgtgatc gaagtgccgg tcggagaggc cgcggctgtc
      121 tgacagaact acaattctcc catgcgacac gctgcatttc cggacggatt gagccgcgac
      181 cgcctcatcg agtggtatcg cgaaacccgc aaggtgacgc gcgccctctt caccatcccg
      241 acggaagagg cgtactacga ccggccgatc cgcctgcgca atcccatcgt cttctatgaa
      301 gggcatcttc cggcattcgc cgtcaacacg ctgatcaagc tggcgctgaa gcgcgagggg
      361 atcgattctg agttggagac gttgttcgca cgcggcatcg atccggaaaa cgaggaagcg
      421 gcgaaaacgc cgaccgattt ctggccaacg cgtgatgagg ttcaacgata tggcgggaac
      481 gccgaggatc agatcatccg ggcattgtgc gacggaccga tcgatgacgg tctcgtcccg
      541 cagcggcgga atgcggaggc cgcgttcacg atcctcgagc atgagctgat gcaccaggag
      601 acgttgctct acatcttcca cgagctgccg tatgagcgga agaacgcgct cgctccgctg
      661 cgcagcggca gaacggccac gcgatcaaca ccggtaacca aagacatggt tcgcatcccc
      721 gccggagagg cgacgctcgg cgctacgggt actgacttcg gatgggacaa cgagatctcg
      781 cagctgcgag tcgccgtcgg cgatttcgcc atcgaccgcc acaacgtcac caacggcgac
      841 tacctcgagt tcgtccgtgc tacgggggcc gaggggccgc acttctggct gaaacgcggg
      901 gatgagtggt actggcgggc gatgttcgat ctcactccgc ttccgctcga ttgcccggtg
      961 tatgtcacgc acgaagaggc gagcgcgtac gcggcctggc ggggcaaacg cttgccgagc
     1021 gagggggaat accaccgcgc tgcgttcgga acgccggacg gggacgagcg ctcgtttccg
     1081 tggggtgatg cgcttcctga cacgagccgc ggaaacttcg gatacgcgaa ctgggacacc
     1141 gtgccggtcg gctcctatcc cgacggtgtc agcgcgtggg gcgtacacga tctcgttggc
     1201 aacggttggg agtggacctc gacgctcttc gagcctttcc caggctttga gccgatgtcg
     1261 tcctatcccg tctattcagc ggactttttc gacgggaagc actacgtgtt gaaaggtgcg
     1321 tcgccggcga ccgctccgga gctcattcgg cgcagcttcc ggaactggta tcggcccgcc
     1381 tacccgtacg tttacgcgac gttccgctgc tgcgcatagc gctccaaacg aatcggccgg
     1441 actcatgcga atcgtcgttc gttaaggcag tctctgcctc agaaatgcgc acgattggtg
     1501 cactgtcgtc gggccatgga aacgatgaca ctcacccaaa cctacaagcc aaaaacgttc
     1561 aacctctcgg gactgactgg catctccgac aagacgctgg agatgcactt caagctttac
     1621 gaaggctacg tgacgaacac caatctcctc agcgagaaga tcgcgtccat tctcgaagat
     1681 gggagcgtcg acaaggagga gatgcccgcc tattcggagc tgacgcggcg cctcggcttc
     1741 gaatacaacg gcatggttct gcacgagtat tacttcggga acatgaagcg caacgcgggc
     1801 ggcaacgtgc ctcggggcag caagttccgc gctgcaatcg aacgcagctt ccccagcttc
     1861 gagatctgga agaccgactt cgtcagcgtc ggcaaaatgc gcggcgtcgg ctgggccatc
     1921 gcctatctcg atccaaccgc aggacgggtt tcgaatcatt ggatcgagct gcatgagaac
     1981 ggcaacgtgg ccggcttcat cccgctgctc gtgatggacg tctgggagca cgcctacctg
     2041 ctcgactaca agccggcaga tcgcccgaaa tacatcgagg cgttcctggc gaacgtcgac
     2101 tgggatgcgg tcgagaagcg gatggcgtaa aggttactgc gcctcgcgaa cctgcagttc
     2161 ggcgggctcg gcgatcacct ggccgtccat ctcgaccgtc cctttgcgct ccagcttgcc
     2221 ccagccggtg cgcatgccgg aggccgcggt cttgagtgat ctgagaacaa cggcatacat
     2281 caactggcga tacagaaagc gctgccagaa caaccagacc aggaccttcg gattttcgcg
     2341 atcgagtttg tatgcgatca gcgacccgat cagttccatc acgaagaaaa acgcgtacat
     2401 gaagccgatg aggtagagcg acgagagtgc tcccggcagc ggctgccagt ctccggcgag
     2461 ccgtccgcgc atccaggccc gcacgactga ggcgatggac cagagaatct gcagatcgat
     2521 cagcggcgac aacacctgga acgcgacctg gaagagccac agcgcaggca gcatcacgcg
     2581 tccaaaccag ccgtagcggc cgaccgcacg ccgatgtttc cagagagact gcagcgtgcc
     2641 gaaggcccag cggaaccgtt gcttgaaaag cgcccggaag ctatcgggag cctcggtata
     2701 tccgaccgcg gccgattccg tctcgatgcg ccagccgata cgccggatgc gccacgtcag
     2761 gtccatgtcc tcggccatcg tgtcggtcga ataaccgccc gcctgcagga tggcctcgcg
     2821 tcgccacgct ccgacagctc cgggaacgac cgtgaccgag ttgatgatcg cgtacgcccg
     2881 gcggtcgaga ttctgactcg tcacgtactc gatcgactgc cagtgtgtga gcggattcat
     2941 ccggttgccg actttgacgt tgccggccac ggcaccgacg agcggatcgg caaaatgacg
     3001 gacgagcttt tcgattgtgt cgcgggcaaa gagtgtgtcg gcgtcgagcg cgatgatgat
     3061 ctcgccggtg gccatggcga tgccgaggtt gagcgcggaa gccttgccgc cgttgggttg
     3121 gacgagcagc gtgactgaat cgccgaagtt cgcgcgcact tcgtccgcgg tgccatcggc
     3181 tgaaccatca tcgacgacga taatttcgag cggcgcgtag ttgttctcga gcaccgcgcg
     3241 aatcgtccgc gcaatcacgg ttgcttcatt gaacgcggcg atgacgacgc tgatggtggg
     3301 cctgactgcg tcgtcgaacc ggcggtgacg ttcgcgccac ttcgcgacga gagcgaggat
     3361 ggtcacgaag acgatgcgcg cagtgcccag aatgatcgcc gtgatgaacg cgacgccgag
     3421 gaagagctca aagaggtaga tgacctcgaa cacgatccgg tcgttggcag ccatcaacgt
     3481 gtcgcccgga ttcaccggag ggttcacctt gtcgcgcgtc gagtcgatga gcgtcgaaac
     3541 agggacgaag gtgtagcctc gtttatgaag ctcgcgcacg aggatcggga tcagcttcac
     3601 ggtctcggtg cggtcaccgc cgccatcgtg caggaggatg caactgccgt gttctcctcc
     3661 atccagttgg gcccaggctg tctcccacat gtcgttagcg ttacgacgat ggatggagcc
     3721 gttgggcatc ttctcttctg tgttccagtc ctgcgtgtcg atgaattcaa ggacggtgat
     3781 gtagttgagc tccgaggcca tcttgatcgg tatgacttcc atggccgacg tcggctctgc
     3841 gtctgcgttg tacggtggac ggaagagcag cgttgaccgg tgaagaaggc tctggaagac
     3901 gcgctgtgtc gcgttcaatt cgaggcgagc ttcctgaggt gggatcaccc cgatgttggg
     3961 atgggtatac gagtggttcc cgatctcatg accttcattc cagatgcggc ggaccagatt
     4021 gggataacgc tcggcgttct gaccgatcag aaaaaatgtc gccggaacgt gcagcgcttt
     4081 cagctcgtcg aggatgtcgg atgtatatgg actggaaggt ccatcatcga tcgtcagcgc
     4141 gatcatcttc ggtttgtatc cggtccggct gatcacgtag gaggtcggaa acttgtgata
     4201 gtcctcgtcg agagcgagtc ccgtttgggg atcgatctcc agtgagcgcg agcctttagt
     4261 cggcaacttg tcgacatgcg caatctcgcc ctcgccgacg aactcgacgt catatgggaa
     4321 gtcgggctcg ctcagcgctt tcatgttgac cggatcattc aggtgatcgc ggtcgatgaa
     4381 actccagatt gacgggtcgg tcgatccgag aacccagatc gccaggccgc ggacgccgta
     4441 gttttgtccg atcagccatt ggttcgcggc agtgaccgca tcgagcatcc agacttcgtg
     4501 ctctttcccg tcgtcgtcct tgtagcgaaa tgtgggattg agtgcggtct catcgaaatc
     4561 gacgatgtct tccggcttct cttcctgccg gtaatcgtgg gccatgacca gagcgccctg
     4621 gtacgtcagc ggctcggccc actcgcgccc ttccatccag tcgtaggagt agttggcgat
     4681 gccgatgacg agcttctcgc gcggaatact ctgcaccgca ttgagaacga cgccgcgata
     4741 ccaggagatc gacgcgatcg gtccgggagg actgttctcc gagtgctcgt cgtaggccat
     4801 gacgacaacg aagtcgcaga ttgacgccac cgcgcgccaa tcgagttttt cccttgcttc
     4861 tttcctggct tcgagatcga tgcttacgag cagccccttc tgcgcaaacg acgtcttcat
     4921 cctcttgagg aacgggatga gaagcggata gtcggcggcg tccaaattct cgaagtcgac
     4981 gttgatgcct tggaagcgca tcgtcacgca ccaacgcttg agatcgagga tcgtcttcgc
     5041 ctggattgcc ggattgttca gaaacagatg aacgcgtttg ggatcgaact caccggagcc
     5101 gtttgtggag acctgggcgt tgctgaagac gggaacgata ttcaagttgt tcgctcgcgc
     5161 cttcttgagt acctcgaggt tgtgtggcgt cagcagggga tcccagtcat gaaaatcgag
     5221 gccctgggca tcagcgcgaa ggtggaccca gaccggtagc aggtgcgtca tcctgctggc
     5281 attggcatcc agcgagtgaa ggccggtttc ctgccacgga gcgtagaagg cggccacgat
     5341 cggcttgccc gtctcaatcg cgcgaatagg ccctttggcc tctcgcgcga gctggttctt
     5401 tcgatcctga cggacgagtg agagaagacg ctctcgtgca ttcttttgaa ggtactgcgc
     5461 tcgagcctgc ttgtgtgccg gcacgcgcag gcctctcctg atgtcttccg taattccgcg
     5521 gaaaccgggc aacagcggta ccgtgaagag actgacgagc cagaagctca ccgtgaccac
     5581 agccatcagg ccgaggagcg tcccgatacg cttgatccac caccagcggc ggtgacttgg
     5641 atcgaaaaat actgcgtggt cgtgcatggc tacgagtgga cgatcatata gaacccgttt
     5701 ggaaacaggg gcattctgcg caacccgggc cgcgttaccc cgtaagacct caccatgact
     5761 cagaccgtca tcgaggtatc aggcctcaaa aagcaatacc ggtcggcggt cggccgttcg
     5821 cggatcaccg ccctggacgg tatcgatttc accgtccgcg aaggggaact gttcggcctt
     5881 ctgggaccga acggcgccgg aaaaacgacc gcagtcaaga ttcttctcga cctcacgcgt
     5941 ccgacggccg gcgaagcccg catcaacggt ctgccggctg gcaatccgga gagccgccgc
     6001 cgggtcggct atctgcccga gggccacaaa atcccgggct acctgactgc gcgccaggcg
     6061 ctggggatct tcggaaagat gtccggcctc gatgacctca cgatcaagcg ccgttcggtg
     6121 gagttgcttg aaaagctgcg gatcgcgcag tggatcgatg tccgcgtcaa gaagtactcg
     6181 aaaggcatga cgcagcgcct cggcctggcc atcgcgctcc tgcactcgcc acacgttctc
     6241 ctgctcgacg agccgaccga tggcgtcgat cctgtcggcc gccgcgagat ccgcgacatt
     6301 ctgcaggccg aagcggcggc gaatgggacg gcggtgctgc tgaactcgca cttgctatcg
     6361 gagatcgagc tgacgtgcaa tcacgtcgcc gttctgcgca acggcaaggt cgccgcggcg
     6421 gggagcatcg aagagctcac gcgccgcgcg gcgaaataca agatggttcc ggccacgccc
     6481 gtcgatgatg cgctcgtcgc ctcgttccgc gagagtggcg caggcgtgga gcgggtcaat
     6541 ggccacatgc tcctgaccgt cgatgacgtc acgcatctga atgcgctcgt tgacaagctg
     6601 cgtgccggcg gcggtttgct gaccgagctt tcacccgtcc gctccacgct cgaagacgtg
     6661 ttcgtcgatc tcgtccgcgc ggtcgatccc ggagcgcagc agtccgaagg agcgctgcta
     6721 tgaaagtcct gatggcgaac atcgaagacg tgatgcgcga ggcggccgcg cgctggactc
     6781 tcgttgccta tttcttcctc tccacgatct tcatcatcat ctttgcctcg gccatcaacc
     6841 tcgacatcgt cgatggcgcg ctggccggcg cgaagctctt cggcaaacag gtcgagatga
     6901 acagctcgat ctcgatcgag aagctcgtga tgggtttcga gtcggggttc tcgggcgtcc
     6961 tttacgttct ctgcacgttt ctggcgatct tcgcgacggc gcatctcgtg ccacgcatgc
     7021 aggagaaggg aacgatcgat ctctatctct cgcggccggt gtcgcgcgtg aagctgatcc
     7081 tctcgcgata cgtggccggg ctcatcctcg ccggcagcaa cgttctttac ctcattggct
     7141 cgatctggat gatcgtggcc tggaagacgc acgttttcca tccgcggttc tttctcgcag
     7201 gcgtcgtgat cctctttctg atcgccacgc tgctcgcgtt cgcattcctc atcggcgtca
     7261 tcacctcgtc gaccgcggtc tcgatcatgg ccacctacgg cgtcttcttc ttcggcgtca
     7321 tgctggccgg ccacgcgcgg atcgaagcag cgctgtcgaa ggaatggcag gcattcgcaa
     7381 tcaagatgct ctactggatc atgccgaaga cggcggagct cgcgacggcg gtcgtcgcat
     7441 atgtagcagg cggtgagatt cacaacgaga tgctcacgat cacgccggct ccattcgtca
     7501 gcaccgcggt tttcggtctg gtctgtctcg ttctcgcttc ctggttgttc caacgaaagg
     7561 agttctaaat gaagaagcag ttagtttccc tcgcagtcct gttgctggcg ctcgctccgg
     7621 ccgcgttcgc aaagaacgac gccatgtccc tcatcccgaa cgattccgtc tccgtaggcg
     7681 ttgtccgcct caacgacatg cgcagcagct cgctctccgc ggcgctcttc gggcagacgg
     7741 caaagatcag caatgacggc gaggcgcaga agttcctgtc tgagtccggc ctccagccgg
     7801 cgaaggacgt cgatgtcatt gtttttgcca cgtcgccccg cacgaacctc ggcagcgaag
     7861 ccgattttct catcgctgct gacggccgct tcaacgtcga gcgtttaacg gctgcgctcg
     7921 tgactcgcgg cgcggtgaag aagacttcgc cgcacggcgt ttactacctg ctgccggaaa
     7981 agggcacgga accccatcat caaggcgtcg ttgcgttccc ggacgcgcat ctcgcgctca
     8041 tcggcagcga agcggcggtt gtcggggcgc tggccaacca cgcagctggc ggaacctcgt
     8101 ttgcgaccgc cagcggcctc ggacgcgaca tggtccgcat tgacccgagc gcgaccgcgt
     8161 tcgtgctcgt cgacgtgccg cgcgcgcagc gcatcaccgg cgcgccgaag ttgaacggaa
     8221 acagctcgtc gtcgcaggca cttggcgcgg cgttgaagaa cgtctcgacc gtcgcgatct
     8281 gggcgaccga cacgggcgac tcactgaagc tcaacgcgat cggtctgtcg accgacgccg
     8341 aaactctggg tctcgtcgaa gacacgctgc gtggcgctct ttcggcgatg cgtctggccg
     8401 tgcaggacaa atcacccgag atggtgtcca cgttgcgcaa gttcacggtg agccgcaccg
     8461 acaactcagt ctcgatctct ggcagcatct ccggcgaggc attccgtact tgggttgcga
     8521 agtcgcaggc gcacggcagc atgcggtagg cagtttctga cgattgaaca tccgtagggc
     8581 cggccgcgcg ccgggcccta ctcgtttctt gatgtcaatg cgcagtccgg cgacgtggcg
     8641 gcaattcggg gcctaccgaa aatgcaggca ccatcgtcac tgtcgtggtg ttgccggtga
     8701 cgtccgcaag atcgaacttc aagtcgacca gtgaccggtc gacgttcgtg atggcgccga
     8761 ggtcgactcg atagaggatt cccgcgccat gagccgtgtc ttcggtgacc tgggttgcgg
     8821 tcaacgggcg ccaggccgtc tcgcctccgt atcgatacga caccttcgtt gcgtcgctgc
     8881 ggacctgctg gtacacgcgt ccgctgatgc tggtgctgcc gatgtagtcc gcggcggaga
     8941 agagaaggga gccgcttccg tgcggttcaa ggcgcgttac aacggcgccc cctccgtcga
     9001 gcaacatcat ggtcgttacg acgggtggaa agtagtcggg ctgcgacgaa tcgacgttca
     9061 tcgtcgccgt tgtcgtcttg tttacgccgg gagcgagcgt tcccagattg gccacttcga
     9121 tgcgataccg ccccggagga gtcgtcagat cgatggaggt cgagtagccg ctgctgagaa
     9181 tccggtttcc gtttgcgtcg aagaacgcag tcgtggtgcc ggtgcgatcg gtaacgcgcg
     9241 actcgcccag cggaccgaca atatccg
//
LOCUS       DATLVW010000176         4816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_460540, whole genome shotgun sequence.
ACCESSION   DATLVW010000176 DATLVW010000000
VERSION     DATLVW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460540"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VNN25_05675"
     CDS             complement(<1..903)
                     /locus_tag="VNN25_05675"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HXH91049.1"
                     /translation="MIMLTSTIAVFLTCAGFVASGLVNFRKRLLGDITATAEMISSHS
                     TAALTFGDRHAAQEVLNALTAKPSVIAAAIYTSRGEPFARYEPKASIHIPQKLQPDGF
                     YEGSDRFELFHSIRLDHERVGTVYIATDASDRNIRLLQYAEIGTTIVLISLLTAFLVA
                     AWLQRSLSGPIVELAHVAGHVSEHKDYRVRATPPNANSGDEIANLVAGFNSMLTEIEH
                     RDDRLLLIQTHLEKTVARRTGELTVANEELLEAKNAAEKIAEINAHLARESALILNNA
                     TDGILVIGLDNRATFINPAGSRMLR"
     gene            complement(945..1544)
                     /locus_tag="VNN25_05680"
     CDS             complement(945..1544)
                     /locus_tag="VNN25_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HXH91050.1"
                     /translation="MALLSPAARARPTHGARLVRSRLVCVVLAVGLSARSVGAQSVAD
                     EYQVKAAYLLNFARFVEWPADVLPASSPLEIGIVGDNPFGMAIDETLRGKSANGHPIH
                     LRHLHWDDVLTSCQIIFISTSEEAHLPDILRNLGQNSILTVSDIDRFSLRGGVIEFRM
                     VGNRVRFDINREPAIAARLSISSKLLSVARAVHEGSASR"
     gene            complement(1532..3550)
                     /locus_tag="VNN25_05685"
     CDS             complement(1532..3550)
                     /locus_tag="VNN25_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH91051.1"
                     /translation="MPNAGKICERVCSTVTAVRRKVLAAGFIVLSLAAGTLQAQENRD
                     LSSLSVDDLMNVEVTSVSRRGQKLSDTAAAVFVITQDDIRHSGAMSIPEVLRIVPGLD
                     VARINGNTWAISARGSNGQYANKLLVMIDGRTVYTPLFAGVFWDVQDTLLDDVDRIEV
                     IRGPGGTLWGANAVNGIINIITKHAIETQGTLVTAGGGAVDGSSAAVRYGGSLGKNGN
                     YRVYAKTFDRPDSTGAATPIHDAWKLGHGGFRADWASRGGDNFTIQGDVYRGHAATGG
                     GGFVDPANPFDIRPASTKVSGDDLLFRWTAIQSSRSDTTLQVFVDDTGRNKPTFDVKR
                     RTADIDFNHHLELGTNDIVWGAEFRRSNDRAAGPGLQLVRDSSTAATTSAFVQDEIAV
                     AHSLHVIAGTKVQYDSLSHLQFQPTLRILYRVNERQTLWAAATSAIRMPAETELYGKI
                     DVGAFPDGRGNTGLIVVYGNRALQPERVESYEVGYRCQISPNIDIDATAFHNRMRDLL
                     ATATPVPFVDSTGRLIVPLHIVNAMDGRSYGGEFLLTNTLTPNWNVAVGYSFLHMITM
                     DEAGVRSAAITDPRHQLQLRSNMELSRGFSLDTAAFYVGRIGNNVPAYVRLDAQLSWR
                     AARHWELSISGQNLLDGHHAEFAGSPTGQSDAAIPIQRTVNGRIAWRF"
     gene            4040..>4816
                     /locus_tag="VNN25_05690"
     CDS             4040..>4816
                     /locus_tag="VNN25_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH91052.1"
                     /translation="MICKHDLDGRILFVSQSVCDALGMPAAILRKMTIQDLLAKDSTW
                     GFAAYMQRMRRDGFADGIMNIVTRDGRKRTWKYRNALEMDEHSVSVILGVARDVTECE
                     EAFQAVLRSEEYFRSIVENVSDIIAIMELDGSLRYGSPSVCRVLGYSRRALRGMRGLD
                     LIHPDDAAQATRYLARQIGDPAAAQTIELQVRHANGAWRSFEIMATNLVKKGRTAAIV
                     LNARDITGRKFLEAQLTQANRLGALGRLAATVAHEFNNVLM"
BASE COUNT          920 a         1429 c         1535 g          932 t
ORIGIN      
        1 gcggagcatg cggctgccgg ccggattgat gaaggttgcg cggttatcga ggcctatgac
       61 gaggattcca tcggtggcgt tgttcagaat caacgcgctt tccctggcca gatgcgcgtt
      121 gatctcggcg attttctcgg ccgcgttctt tgcttcgagc agttcttcgt tggcgacggt
      181 caactccccg gtgcggcggg caaccgtttt ctcaagatgc gtttgaatca ggagaagcct
      241 gtcatcgcga tgttcgatct cggtgagcat gctgttgaat ccggcgacca gattggcgat
      301 ctcatcgccg ctgttcgcgt tgggcggagt tgcccgaacc ctatagtcct tatgttcaga
      361 gacgtgaccg gcgacgtgcg caagctcgac gatgggcccg gagagggagc gctgcaacca
      421 tgccgctaca aggaatgcag tcagcagcga gatgaggacg atcgttgtcc cgatttccgc
      481 gtattgcagc agccggatat tgcggtcact agcgtcggtt gcgatgtaca cagtaccgac
      541 gcgctcatga tcgagccgga tggaatgaaa cagttcgaag cggtcgcttc cctcatagaa
      601 gccgtccggc tgaagcttct gggggatatg aatcgacgcc ttcggttcgt atcgcgcaaa
      661 cggctcgcct cgggatgtat agatcgccgc ggcgatcacc gatggttttg ccgtaagggc
      721 attcaacacc tcctgggcgg cgtggcggtc tccaaacgtc agtgcggcgg tgctgtgaga
      781 gctgatcatt tccgcggtcg cggtgatgtc gccaagcaaa cgcttccgga aattgacgag
      841 accagaggcc acaaagccag cgcatgtcaa gaagacagcg atggtgcttg tcagcatgat
      901 catgccggtc agttttccgc gaatggagct tctccacctc gcggtcatct cgatgcactc
      961 ccttcatgaa cggcccgggc aacgctcagc aatttggagc tgatgctcag ccgcgcggcg
     1021 atggccggtt cgcggttgat gtcgaagcga acgcggttac cgaccatccg aaactcgatg
     1081 acgccgcccc gaagcgagaa gcggtcgatg tcgcttacgg tgaggatgct gttctgccca
     1141 aggttgcgca ggatgtccgg gaggtgcgcc tcctcggacg tgctgatgaa gatgatctgg
     1201 cacgacgtca gaacatcgtc ccaatgcagg tgccggaggt gaatggggtg accattcgca
     1261 gactttccgc gcagagtttc atcgatcgcc atcccgaagg ggttgtcgcc aacgatcccg
     1321 atctccagag gcgaggaggc gggaaggacg tcggcgggcc actcgacgaa gcgggcgaag
     1381 ttcagcaggt atgcggcctt tacctggtac tcgtcagcga cggactgcgc cccgacgctg
     1441 cgagccgaca gccccaccgc gagcacgacg cagacgagac gcgagcggac gagccgcgcc
     1501 ccgtgagtcg ggcgcgctcg cgccgcggga ctcaaaagcg ccatgcgatt ctcccgttca
     1561 cggtacgttg aatcgggatt gcggcgtcgc tttggccagt ggggctaccg gcgaattcgg
     1621 cgtggtgccc atcaagcagg ttctgtccgg agatgctcag ttcccagtgt ctcgccgcgc
     1681 gccacgacag ttgtgcgtcg agtcgtacat acgccggtac gttgttgccg atgcggccga
     1741 cgtaaaaggc cgcggtgtcg agcgagaatc cgcgcgaaag ctccatgttg gagcgcagtt
     1801 gcagttggtg gcgcggatcg gtgatcgcgg ccgaccgcac gccagcttcg tccatggtga
     1861 tcatgtgcag aaaggagtag ccaaccgcga cgttccagtt cggggtgagc gtgttggtga
     1921 ggagaaactc tccgccgtac gagcgcccgt ccatcgcgtt cacgatgtgg agcggaacga
     1981 tcaaccggcc cgttgaatcg acaaatggga ctggcgtagc cgtggccagg agatcacgca
     2041 tccggttatg aaaggccgtc gcgtcgatat cgatgttggg ggaaatctgg cagcggtacc
     2101 caacttcgta cgattcgact cgctccggct gcaacgcccg attgccatag accacgatga
     2161 gaccggtgtt tccccttccg tcgggaaagg caccgacgtc gatctttccg taaagctcgg
     2221 tctcggcggg cattcgaatc gcgctcgtag cggccgccca gagtgtctgc cgctcgttca
     2281 ccctgtacag aatgcgcaac gtcggctgaa actgcagatg cgagagggag tcgtactgaa
     2341 ccttcgtgcc ggcgatcacg tgcagggaat gagcgactgc aatttcatcc tgcacgaacg
     2401 cactagtggt cgccgcggtg ctcgagtcgc gaacgagctg cagtcctgga ccagcggcgc
     2461 gatcgttgga gcgacggaat tcggcgcccc agacaatgtc gttcgttccg agctctaggt
     2521 gatgattgaa gtcgatgtcg gccgttcgcc gtttgacgtc gaaagtcggt ttgttgcggc
     2581 cggtgtcgtc gacgaacacc tgcaaggtcg tgtccgaccg gctggattga atcgccgtcc
     2641 agcgaaagag aagatcgtcg cctgagactt tggtggacgc gggccggatg tcgaaggggt
     2701 tggcaggatc gacaaatcct ccgcccccag tggccgcatg tccgcgatag acgtctccct
     2761 ggatcgtgaa attgtcgcct ccgcgtgagg cccagtcagc gcggaagccg ccgtgtccga
     2821 gcttccaggc atcgtgaatc ggagtggcgg ctccggtcga atcggggcgg tcgaatgtct
     2881 tggcgtagac gcggtaattg ccgttctttc ccagcgatcc gccgtaacgg acggccgccg
     2941 acgatccgtc gaccgcgcct ccgccggctg tgaccaacgt tccctgcgtc tcgatcgcgt
     3001 gcttcgtgat gatgttgatg atgccgttga ccgcattcgc tccccaaagt gtgccaccgg
     3061 gaccgcggat gacctcgatg cggtcgacgt catcgagaag agtgtcctga acatcccaga
     3121 agacgccggc gaagagcggc gtgtagacag tccgcccgtc gatcatgacg aggagcttgt
     3181 tcgcgtattg tccgttggag ccacgcgccg agatggccca ggtgttcccg ttgatccgcg
     3241 ccacgtccag ccccggcacg atgcgcagga cctccggaat gctcatcgcc cccgagtggc
     3301 ggatgtcgtc ctgcgtgatc acgaagacgg cagcggcggt gtccgacaac ttctgccctc
     3361 ttcgcgacac cgaagtcacc tcgacgttca tcaggtcgtc gacgctgagc gaggagagat
     3421 cgcggttttc ctgtgcctgg agtgttcccg cggccagcga caagacgatg aaccctgcag
     3481 ccagaacttt ccggcggaca gcggtgacgg ttgagcagac gcgttcgcag atttttccag
     3541 cgttgggcac aagcacttcc tttgtttgag acctcgggac agggtcaact gggaagtgcg
     3601 ctggggagcc gggaaaaata cagggtgtgg gggcggggtg tagggggtgg gatgtggagc
     3661 gtaggggttg gtgatccctc gcgacacagt cttttcacgc ggagacgcgg agaagcgggg
     3721 aggctttgaa cctttcctcc gcgtcctccg cggctccgcg tgaccgggtt tttgtgggtc
     3781 cagcgacgag cttgcctccc aacccgtgat cccccactcc ccactccctc gcctgtattt
     3841 ttccaaccct tctccggcac tcccattcag cgcggtgagt ccgccgcagt ccccatcaaa
     3901 ggagccggcc acaagccgtc accgaacaat gctcgaacac caattgaacg caatcagtca
     3961 ttccgaggga gcgatgccga gagggcagga aacatttgat gaaacaggca gaagcgagcc
     4021 ggccgcgccg agcgccgatt tgatttgtaa acatgatctc gacggccgca tcctcttcgt
     4081 cagtcagtcc gtctgcgacg cgctcggcat gcccgccgcc attcttcgca agatgacgat
     4141 tcaggatctc ctggcgaagg acagcacgtg gggcttcgcg gcctacatgc agagaatgag
     4201 gcgcgacggc ttcgcagacg gcatcatgaa tatcgtcacg cgcgacggca gaaagcggac
     4261 ctggaagtac cgcaatgcgc tcgagatgga tgagcactcc gtgtccgtca tcctgggcgt
     4321 cgcacgcgac gtgaccgaat gtgaggaagc tttccaggcc gtattgcgaa gcgaagagta
     4381 cttccgctcg atcgtcgaga acgtctccga catcatcgcc atcatggagc tcgacggcag
     4441 cctccgctac ggcagtccct cggtctgcag agttctcggc tactcgagga gagcgcttcg
     4501 tggcatgcga ggcctcgatc tgatccatcc ggacgatgcg gcacaagcga cgagatacct
     4561 ggcgcgccag atcggggatc cggccgctgc ccagaccatc gaattgcagg tccgccacgc
     4621 caacggtgcc tggcgctcct tcgagatcat ggccacgaat ctggtcaaga aggggcggac
     4681 cgcggccatc gtgctgaacg cccgcgacat caccggacgc aaattcctgg aggcgcaact
     4741 gacgcaggcc aaccgcctcg gcgcactcgg ccgcctcgcc gccaccgtcg cgcacgagtt
     4801 caacaatgtg ctgatg
//
LOCUS       DATLVW010000177         4869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_6486, whole genome shotgun sequence.
ACCESSION   DATLVW010000177 DATLVW010000000
VERSION     DATLVW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6486"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VNN25_05695"
     CDS             <1..878
                     /locus_tag="VNN25_05695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXH91053.1"
                     /translation="PSSGSVAIIEMLHMLEHYDVGSMGWQSARYLHLLTEVMRRAFAD
                     RAEFLGDPDFNTMPIAALTSAKYAEERRSTIDLERASSSKDIRAGDPAPFEKTDTTHF
                     SIVDADGSMVSNTYTLNDWFGSGVTAKGTGVLLNNEMDDFTSRPGVANEYQLIQSAKN
                     AIAPKKRPLSSMVPLIMLDDGKPWLAVGAAGGPRIISTVLEIVLAVVDFHINIQQAVD
                     AGRIHHQWMPDEIYWEPDGTNPDTRSILETMGHKFHEKGLDHISDANAVMIDPKTGLR
                     QGGSDARRSGVAAGY"
     gene            895..2265
                     /locus_tag="VNN25_05700"
     CDS             895..2265
                     /locus_tag="VNN25_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH91054.1"
                     /translation="MNLDKKSLLAWAQDHRADFESTLQQFVEIPSVSSEPERADDIRR
                     CAELAAAKIRGFGGVASILETEGNPIVHGRFDVDPSLPTVTVYNHLDVQPASAETEPW
                     KNDPFTFIKEGDRYLGRGTTDDKGPALTALFAIRAAHEAGVRVNIHTIWELEEEIGSP
                     NFEGAMKKYAEQLATNSVLVSDTIWVSRERPACPAGLRGLQGFELSLETAKTDQHSGV
                     VGGAARNPIGELMKLVTKMYNATTGKVKIKGFYDDVMQPSKKELNDFKNSGFTVKKFK
                     KDHGFKSIRTEDPMEVMKRIWALPTMEVHGLVGGYTGAGIKTVVPPRATVKISCRLVP
                     NQDPKKIKKLVRDFVKQHNPDVKISFEASMEAYQAPTTGPLANAVRSAMKFAFGREPV
                     FVREGGSIGAVVSMEQVLKAPVLFLGLSLPEHGYHAPNENYDWQQASGGMVAFVKYFE
                     EIAGMK"
     gene            complement(2339..2581)
                     /locus_tag="VNN25_05705"
     CDS             complement(2339..2581)
                     /locus_tag="VNN25_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91055.1"
                     /translation="MPTRLEQFLKSHRIKPAHLARESGYSRQHLLRIRMGRMEPTRRC
                     IAAIVAACRHLANEPVLANDLFDLGEEERQPKIADI"
     gene            2701..3510
                     /locus_tag="VNN25_05710"
     CDS             2701..3510
                     /locus_tag="VNN25_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH91056.1"
                     /translation="MLGETVRAARLERGLTQERLARLAGVSRRHLAALEKGANVSVAV
                     LRKVATVLQLAEINLGELTVRTDQSTSTSVNMPVLAETIREAHAGTLRAQVLLARAGS
                     ILGDDEAGPSSVVVRFPKMQPLRIEGKGRRRGANAIADRPGESRVPIAGEIRQGAEVV
                     ELSGENAPLPSAWIAAGEKVFRARGDLLQAVGIEDGDLLIVELRPTGRAASGELVIVK
                     AGELLYVGHWWQKHGRKSLMTDGLSEVTTGGTASKRALKVMAAINAIVRVR"
     gene            complement(3932..4174)
                     /locus_tag="VNN25_05715"
     CDS             complement(3932..4174)
                     /locus_tag="VNN25_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXH91057.1"
                     /translation="MDWKEMITADPEVMVGKPVIAGTRLTVEFVVERLADGWTEEQLL
                     ENYPGLKREQIHACLAYAAEMLSEVQMYSLPLRRHA"
     gene            4346..>4869
                     /locus_tag="VNN25_05720"
     CDS             4346..>4869
                     /locus_tag="VNN25_05720"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXH91058.1"
                     /translation="MRRSLLLIAFAGLAAIQAGCGSSHTAAPVAATTAPPVAAYFAPH
                     GFDLTAMDTSVKACDDFYRFAVGKWRDTHPLPAQYSRYGRFEELSERNRDVLHQIAEQ
                     DAALTTAAPGSAEQKVGDFYASCMNEAAIEAAGVTPIQPELDRINAVSDRSSLLAEIT
                     HLQTAGYAPLFRVGG"
BASE COUNT          981 a         1482 c         1513 g          893 t
ORIGIN      
        1 caccgtcgtc cggaagcgtg gcaatcatcg agatgctgca catgctcgag cattacgacg
       61 tcggctcgat gggctggcag tcggcgcggt atctccatct gctgactgaa gtgatgcgta
      121 gggctttcgc ggatcgcgcg gagtttctcg gcgatcctga cttcaatacg atgccgatcg
      181 ccgcgctgac gtcggcgaaa tatgccgagg agcggaggag cacgatcgat ctggagcggg
      241 cgtcgtcgtc gaaagacatt cgggccggcg atccggcgcc gttcgagaag accgacacga
      301 cccacttttc gatcgtcgat gccgacggca gcatggtcag caacacctac acgctgaacg
      361 actggttcgg cagcggcgtc accgcgaaag gaacgggcgt gctgctgaac aacgagatgg
      421 acgacttcac ctcgcgtccg ggcgtggcga acgaatatca actcatccag agcgcgaaga
      481 acgcgatcgc gccgaagaag cggccgctct cctcgatggt gccgctgatc atgctcgacg
      541 atggcaagcc gtggctcgcg gtcggcgccg cgggagggcc gcgcatcatc agcacggtcc
      601 tcgagatcgt gctggccgtc gtcgactttc acatcaacat ccagcaggcc gttgacgccg
      661 gccgcatcca ccatcagtgg atgccggacg aaatctattg ggagccggac gggacgaatc
      721 ccgacacgag gtcaatcctg gagacgatgg gccacaagtt tcacgagaag gggctcgatc
      781 acatctccga cgccaatgcc gtgatgatcg atccgaaaac gggcctgcgc caggggggat
      841 cggatgcccg ccggagcggc gtggcggctg gctactgaca tagaatccgc cgctatgaat
      901 ctcgataaga aatcccttct cgcatgggcg caggatcacc gcgccgattt tgaatcgacg
      961 ctgcagcagt tcgtcgaaat cccgtcggtt tcctccgagc ctgagcgcgc cgatgacatc
     1021 cgccgatgcg cggagctggc cgcggcgaag atccgcggat tcggcggcgt agcctcgatc
     1081 ctcgaaacgg aaggcaatcc gatcgtacac ggacgtttcg acgtcgatcc ctcgcttccc
     1141 acggtcaccg tctacaacca cctcgatgtg cagccggcgt ccgccgagac cgagccatgg
     1201 aagaacgatc ccttcacatt catcaaagaa ggcgaccgct acctcgggcg cggcaccacc
     1261 gacgacaagg gtccggccct caccgcgctg ttcgcgattc gcgcggcgca tgaggcgggc
     1321 gtgcgcgtca acatccacac gatctgggag ctcgaggaag agatcggatc gccgaacttc
     1381 gaaggggcga tgaagaagta cgcggagcaa ctcgccacga actccgtgct cgtctctgac
     1441 accatttggg tctcgcgcga acggcctgcg tgcccggcag ggctgcgcgg tctgcagggt
     1501 ttcgagctgt cgctggagac ggcgaaaacc gatcagcact ccggcgtcgt cggtggcgca
     1561 gcgaggaatc cgatcggcga gctgatgaag ctggtcacga agatgtacaa cgccacgacc
     1621 ggtaaagtga agatcaaagg cttctacgac gacgtcatgc agccgtcgaa aaaagagctg
     1681 aacgacttca agaactcagg tttcaccgtc aagaagttca aaaaggacca cggcttcaag
     1741 tcgatccgca ctgaagatcc gatggaagtg atgaagcgga tctgggcgct cccgacgatg
     1801 gaagtacacg gcctcgtcgg cggctatacc ggcgccggca tcaaaacggt cgttccgcca
     1861 cgggccacgg tcaagatctc ctgccgcctg gtgccgaatc aggatccgaa gaagatcaag
     1921 aagctcgtcc gcgatttcgt caagcagcac aaccccgatg tgaagatcag cttcgaggca
     1981 tcgatggagg cgtatcaggc tccaacgact ggtccgctcg cgaacgccgt ccgcagcgcg
     2041 atgaagttcg ccttcggccg cgagccggtc ttcgtccgcg aaggcggttc gatcggtgcg
     2101 gtcgtctcga tggaacaggt cctcaaggca ccggtcctct ttctcggcct ctcactcccc
     2161 gaacacggct accacgctcc gaacgagaac tacgactggc agcaagccag cggcgggatg
     2221 gtggcgttcg tgaagtactt cgaggagatc gcggggatga agtaaaggag gtcacacaga
     2281 ggggcagaga gtacggaagt gaactgttat ctctgtgact tctacgagcc tgtggtgatc
     2341 aaatgtcagc gattttcggc tgccgctctt cttcgccaag gtcgaaaagg tcgttggcga
     2401 ggaccggctc atttgccagg tggcggcagg cggcgacgat cgctgcgatg cagcggcggg
     2461 tgggctccat ccttcccatg cggatgcgca ggaggtgctg ccggctgtag ccggattccc
     2521 tcgccaggtg ggctggtttg atccggtggc tcttgagaaa ctgttccagt cgcgtcggca
     2581 tgtgcaatat agtgcatcat aatccgcggc atgatgggtg tcaatcggta agtgcggagc
     2641 actcctagat gagttgctta gattacgcga acctagtaac gtataagtta cttggcaccc
     2701 atgctcggag aaaccgtacg tgcggcccgc ctggagcgag gcctgactca ggagcggctg
     2761 gcccgccttg cgggtgtcag ccggcggcat cttgctgctc tggagaaagg cgcaaatgtg
     2821 tcagtcgcgg tcctgcgcaa ggtagcgacc gtgcttcagt tggccgagat caacctgggc
     2881 gagctgaccg tccggacgga ccaatcgacg tcgacgtccg tcaatatgcc ggtgctggcc
     2941 gagacgatcc gcgaggcgca cgcaggcact ttgcgggcgc aggtgctctt ggcccgcgcc
     3001 ggatcgattc tcggcgatga cgaggccggt ccctcgtccg tcgtcgtgcg ttttccgaag
     3061 atgcagcccc tgcggatcga aggaaagggt cggcggcgtg gggcaaatgc gatcgccgac
     3121 cgtcccggag aatctcgagt cccgatcgca ggcgaaatcc ggcaaggcgc ggaggttgtg
     3181 gagctctccg gcgagaacgc cccgctcccg tctgcatgga tcgcggcagg tgagaaagtc
     3241 ttccgcgctc gcggcgatct tttgcaagcg gttggaatcg aagacggcga cctgctgatc
     3301 gtcgaattac gtccgactgg ccgcgccgct tcaggcgagc tcgtgatcgt gaaggcgggc
     3361 gagctgctct acgtcggtca ttggtggcag aaacacggcc gcaaatcgct catgacggac
     3421 ggcttgtccg aggtgacgac aggcgggacg gcgtccaagc gtgcactgaa ggtgatggcc
     3481 gcgatcaacg cgatcgtgcg ggttcggtag gagagcgttg cagaattaca aggttgcagc
     3541 ggtgcaggtt ccgccgctat aggtcttcct tggtaacccg gccactggct aaggaagcgg
     3601 cgtcttgcga acctcgtgtt ctcaatcacg cagaaaagac gatggaggtt ctccctctcg
     3661 ctctccacga ggtccatcac cagaacgacg gtgtcttgca tgctcgtctc gcgcagccga
     3721 atcagcacga cgcccaccga atggcctcgt ctgaaaacaa gctcgccgaa gtctttgtcg
     3781 aatgtgatga ccacgcgccg ttcgtgcgcc gacttttcaa tcagatcgac gtcgggagtg
     3841 cgtggaacgg agtaagccca gaacacttcg tggccggatg aggtcagcgt ctcaacgagc
     3901 agtcccggcg tgttggcgtc cgcgagaaac ttcatgcatg ccggcgcaac gggagcgagt
     3961 acatctgaac ctccgaaagc atctcagcgg cgtacgccaa gcacgcgtga atctgctccc
     4021 gcttcaatcc cgggtagttc tcgagtagct gctcttccgt ccacccatcg gcgaggcgtt
     4081 cgacgacaaa ctcgaccgtc aggcgagtgc cagcgatgac aggtttgccc accatcactt
     4141 ccggatccgc ggtgatcatc tctttccagt ccatcatgtt tttgcctgtc ggctttccgc
     4201 tccgcaagga taaccgcccg agaaccggaa gtgaaactgg caaaccagca accccagctc
     4261 ccaacctggc accattcgtt cacgtcacgt tccggttacc ttgcatacga tcgcgccgaa
     4321 aaatttggac cccaatggag gaactatgcg ccgttctctt cttctgatcg cgttcgccgg
     4381 attggccgca atccaagccg gatgcggatc gtcacacacc gctgcacccg ttgctgcgac
     4441 aaccgcgccg ccggttgccg cctacttcgc gccgcatggt ttcgacctga ctgcgatgga
     4501 tacgagcgtc aaagcctgcg atgacttcta tcgattcgcc gtcggcaagt ggcgcgacac
     4561 gcatccgctt ccggcgcagt actcccgcta cgggcgtttc gaagagttgt ccgagcgcaa
     4621 ccgcgatgtg ctccatcaga tcgccgagca ggatgcggcg ctcaccaccg ccgctcccgg
     4681 cagcgcggag cagaaagtcg gcgacttcta cgcctcctgt atgaatgaag ccgcgatcga
     4741 ggctgccggc gtcacgccga tccagccgga gctcgatcgg atcaacgccg tcagcgaccg
     4801 gtcctcgctc ctcgccgaga tcacgcatct tcagacggca ggttacgcgc cgctcttccg
     4861 cgtcggcgg
//
LOCUS       DATLVW010000178         5828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_134835, whole genome shotgun sequence.
ACCESSION   DATLVW010000178 DATLVW010000000
VERSION     DATLVW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134835"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VNN25_05725"
     CDS             <1..660
                     /locus_tag="VNN25_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXH91059.1"
                     /translation="LEQNPWDTIAERYPIGAVVSGKVRNLTDFGAFVEVEDGIDGLIH
                     ISDMTWNRRLKHPSEVLKKGDAVQARVISVDAENQRLSLSIKEFLPNEWDTFAKTHNV
                     GDEVIGTVSKITDFGLFIRVADGVEGLAHVSEISRDPKAKLEKSFHPGSPVRAKVIKI
                     DWPDKKIGLSTRDVEPLTEEEMAHHAEAARVAEESHVHEPETHSESETETPTPETTPQ
                     G"
     gene            664..1632
                     /gene="sppA"
                     /locus_tag="VNN25_05730"
     CDS             664..1632
                     /gene="sppA"
                     /locus_tag="VNN25_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXH91060.1"
                     /translation="MSELDPVLPAGEPIVATPPRKSRAGIFFFGAFSGCLVVVIGFLI
                     LGVFIASFAGNDTTSRGDVFGDKVAIIPIDGEILGSREAIEALHRYARNSNVKAIIMR
                     INSPGGAIAPSQEIYEEIRNVRAHSGKPIIASLDSVAASGGYYIASACDRIVANPGSI
                     TGSIGVILQWMETKDLLAWAKLKPETITSGPMKAAGSPYQDLTDAQRAYFQDIVTQLH
                     SQFVRAVAAGRKGKLTEAEVGKIADGRVFTGEQALGLKLVDELGNLDDAINVAGKLGG
                     IHGRPGTIYPKKRTRGLFDALTSDDSDTETAIGRILSRRPRFLYQW"
     gene            1656..2042
                     /locus_tag="VNN25_05735"
     CDS             1656..2042
                     /locus_tag="VNN25_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HXH91061.1"
                     /translation="MDHLEPEHELEDKAGVMTKAELVDEVARVVQLTKKQAETIVNIV
                     FDSIVDSLRSGQKIELRGFGSFRLRSRKSRTGRNPKTGEKVEVPSKKIPYFKPGKELK
                     ELINQVLEASSAARTESSAKAANEEG"
     gene            2060..2635
                     /locus_tag="VNN25_05740"
     CDS             2060..2635
                     /locus_tag="VNN25_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXH91062.1"
                     /translation="MNENIRRPRVILHRSSFILFMEILFTPWRYSYLTSPKSEASPGC
                     IFCNAARAEGLRETQTLFRNADALVMLNRYPYTNGHLMVAPVAHDALLFDSRDDSLRA
                     LIRLTAEAQRILSDVYHPEGFNVGMNFGQVAGAGVADHYHMHIVPRWGGDANFMTVTA
                     RTRIVPEDLEVTYERLLPHFQQIRKESLSIQ"
     gene            2632..3003
                     /locus_tag="VNN25_05745"
     CDS             2632..3003
                     /locus_tag="VNN25_05745"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HXH91063.1"
                     /translation="MKGRTIFAMLLAYLIPGAGHLYLGKYARAATFFCVVALMFVIGI
                     SIDGDLYMLGHTGGSLLRLLAALGSLGAGVIYWIAAWMGVHGDVTSITFEYGTAFTIT
                     AGLMNLLLILDAFDIAQGRKE"
     gene            3000..3185
                     /locus_tag="VNN25_05750"
     CDS             3000..3185
                     /locus_tag="VNN25_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91064.1"
                     /translation="MSLLRNHIVLMFLYALGTGIFFALLWKETRAERIRFFLLVFFSL
                     FLGGIALAWMMYPFPLR"
     gene            3182..4288
                     /gene="lpxB"
                     /locus_tag="VNN25_05755"
     CDS             3182..4288
                     /gene="lpxB"
                     /locus_tag="VNN25_05755"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXH91065.1"
                     /translation="MKLAVIAGEASGDLHAAEVVRELKVLDPALEVFGIGGDLLAAEG
                     VRLIHHAREMGIVGLFNVLRHLRMFRRIYRDTIDEIAQQKPDAVLLVDYPEFNLRVAE
                     RCKAMGIRVIYYISPQVWAWRKGRVKKIARIVDLMIVIFPFEETFYREHDVPVVYAGH
                     PLIDELAGVARGDHEPGTPLRLALLPGSRRGEVSQLLPAMLDAVANLRRERELDAFVI
                     QATTISSAELLAIMQTANVYVRIVPHGGGEELASADVALSSSGTATLECAVIGTPVIV
                     MYRLSTATLWFARRLVSLQHFSLVNILAGKKVVPELLQHDVNGDRIAAEVRALAQPEN
                     HARVRAELTAVRALLGKGGASHRAAEAIMSAVHR"
     gene            4285..>5828
                     /locus_tag="VNN25_05760"
     CDS             4285..>5828
                     /locus_tag="VNN25_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91066.1"
                     /translation="MTVLRRLYRYLRHYKAWAALAFGSMIIFAVTQTAMIGLVQPIFD
                     IGLTPPSSKPYAETHQSKEEKVKSGILDTVLEREKPEGQRGWLINHGDELNHQFHGWW
                     DKASRKIQFRRLLLAMLIVIILRTFASFFSEYAFQKVGLSTVRDLRNELYERLIFQSH
                     RFFSERSTGEMVSRVVSDADAIQAAVSTRMGDLFQESLTLFFLLVYVFWSNPELALIT
                     FIAAPLLVFPVVHFGKRLRGTTHRSQERMADMATLLEETIRGVRIVKAFTMERFEISR
                     FRDATKRHLSSNLKAQKIQALSSPVMDFITGIGGLSLFLYAQRRIGNGTLSIGALMAY
                     MAAVAAMYQPMKKLNKVNLSVNTALSAAERVFRMLDIPNEVEDRPKAETISAVGSGIC
                     YENVSFQYEEEPVLRDVGLTVAPGEIVALVGGSGAGKSTFLNLLPRFYDVNAGRITID
                     GHDIRDLQLSSLRGLMGLVTQEVVLFNDTVRNNIAYGRGDVDSERVIDAAKAANAHDF
                     ISAMPQG"
BASE COUNT         1122 a         1839 c         1744 g         1123 t
ORIGIN      
        1 ctcgagcaga atccgtggga cacgattgcc gagcgttatc cgatcggcgc agtcgtctca
       61 gggaaggtcc gcaacctcac ggacttcggc gcgttcgtgg aggtcgaaga cggaatcgac
      121 ggtctgatcc acatctccga catgacctgg aaccgccggc tcaagcatcc gagcgaagtc
      181 ctgaagaagg gcgacgccgt gcaggcccgc gtgatcagcg tcgacgcaga gaaccagcgg
      241 ctgtcgctct cgatcaaaga gttcctgccg aacgaatggg acaccttcgc caagacccac
      301 aacgtcggcg acgaggtgat cggaacggtc tccaagatca ccgacttcgg tcttttcatc
      361 cgcgtagcgg acggcgtgga agggctcgcg catgtctccg agatttcgcg cgatccgaag
      421 gcgaagctcg aaaagtcgtt ccatccgggc tctccggtgc gcgccaaggt gatcaagatc
      481 gactggcccg acaagaagat cgggctgtcg acgcgcgacg tcgagccgct cacggaagag
      541 gaaatggcac accacgccga ggcggcgcgt gtggccgaag aatcgcacgt tcacgaacca
      601 gaaacgcatt cagaatccga aacagagact cccactccgg agacgacgcc gcaaggctga
      661 tccatgtcgg aactcgatcc cgtactccca gccggtgagc cgatcgtggc aacaccgccg
      721 cgcaaatccc gcgccggtat tttctttttc ggcgcgttca gcggctgtct ggtcgtcgtc
      781 atcggtttcc tgattctagg agtcttcatc gcgtcgttcg ccggcaacga cacgacgtcg
      841 cgcggcgatg tcttcggcga taaggtggcc atcattccga tcgatggcga gatcctcggc
      901 tcgcgcgaag cgatcgaggc gttacaccgc tacgcgcgca actccaacgt caaagcgatc
      961 atcatgcgga tcaacagccc cggcggagcc attgcgccgt cgcaggagat ctacgaagag
     1021 atccgcaacg tccgcgcgca cagcggcaag ccgatcatcg cctcgctcga cagcgtcgcg
     1081 gcatcgggcg gctactacat cgcctcggcc tgtgaccgta tcgtcgccaa tcccggctcg
     1141 atcacgggat cgatcggcgt cattctgcag tggatggaaa cgaaagatct gctggcctgg
     1201 gcgaagctga agccggagac catcaccagc ggtccgatga aagcagccgg atcgccgtat
     1261 caggatctga ccgacgcgca gcgtgcctat tttcaagaca tcgtcacgca gctccactcg
     1321 cagttcgtac gtgccgtggc cgctggccgc aaaggaaaac tgaccgaggc tgaagttgga
     1381 aagatcgccg acggccgcgt cttcaccggc gagcaggccc tcgggctcaa gctcgtcgat
     1441 gagttgggca atcttgacga tgccatcaat gtagcaggca agctgggcgg cattcacgga
     1501 cgtcccggca ccatctatcc gaagaaacga acgcgcggct tgtttgatgc gctgaccagt
     1561 gacgactccg atacagagac cgccatcggc aggattctca gccggcgtcc gcgttttctc
     1621 tatcagtggt gaccgtcatg agtgaggagg cacctttgga tcacctggaa cccgaacacg
     1681 agctcgaaga taaagcgggc gtgatgacca aagcggagct cgtcgacgag gtggcccgcg
     1741 tcgtccagct cacgaagaag caggcggaga cgatcgtcaa catcgtcttc gattcgatcg
     1801 tcgattctct ccgctccgga cagaagatcg agctccgcgg cttcggaagt ttccggctgc
     1861 gcagccgcaa gtcgcgcacc ggccgcaatc ccaaaaccgg cgagaaggtg gaagtaccgt
     1921 cgaagaagat cccgtacttc aagccgggca aagagctgaa ggagctgatc aatcaggtgt
     1981 tggaggcctc gtccgctgct agaaccgaat catctgcgaa agcagcgaac gaggaaggat
     2041 gaacgatgaa ccatgaggga tgaatgagaa tatccggcgg cctcgggtca tccttcatcg
     2101 ttcatccttc atccttttca tggaaatcct gttcactccc tggaggtact cctacttgac
     2161 ctcgccgaaa agcgaggcct cacctggttg catcttctgc aacgctgcca gagcggaggg
     2221 ccttcgtgag acccagaccc tcttccgcaa cgcggacgcg ctggtcatgc tgaaccgcta
     2281 tccctacacg aacggccatc tgatggttgc gccggtggcg cacgatgccc ttctgttcga
     2341 cagccgcgac gattcgttgc gcgcgctgat ccgcctcacc gccgaggcgc agcgcattct
     2401 ctccgacgtg taccatcccg aaggattcaa cgtcgggatg aacttcggcc aggttgccgg
     2461 ggccggagtg gccgatcact accacatgca catagtcccg cgttggggag gcgatgccaa
     2521 cttcatgacc gtcaccgccc gcacgcgcat cgttcccgaa gatctcgaag tcacctacga
     2581 acgccttctc cctcacttcc agcaaatccg aaaggaatct ctctccatcc aatgaaaggc
     2641 cgcacgattt tcgcgatgct gctggcgtac ctcattccag gcgccggaca tctctacctc
     2701 ggcaagtacg cccgggccgc caccttcttc tgcgttgtcg cgttgatgtt cgtcatcggc
     2761 atctcgatcg acggcgatct ctacatgctc ggtcacaccg gcggttcgct gctgcggctc
     2821 cttgccgctc tcggctccct cggcgcggga gtgatctact ggatcgccgc gtggatgggc
     2881 gttcatggcg acgtcacctc catcacgttc gaatacggca cagcgttcac gattacggca
     2941 gggctgatga atctgctcct gatcctcgac gccttcgata tcgctcaggg tcggaaagaa
     3001 tgagccttct ccgcaatcac attgtcctga tgttcctcta cgccctgggc acgggcatct
     3061 tcttcgcgct gctctggaaa gagacgcgcg ccgagcggat tcgcttcttt ctgctggtgt
     3121 tcttctcgct ctttctgggc ggaatcgcgc tggcctggat gatgtatccg ttcccgctgc
     3181 gatgaagctt gccgtcatcg ccggcgaggc ttccggcgat cttcacgccg cagaagtagt
     3241 ccgtgagctg aaggttctcg atccggcact ggaggtgttc gggatcggcg gcgacctgct
     3301 ggccgccgaa ggtgtgcgct tgattcatca cgccagggag atgggcatcg tcggcctctt
     3361 caacgtgctc cgccacctgc gcatgtttcg cagaatctat cgcgacacga tcgacgagat
     3421 cgcgcagcag aagccggatg ccgtgctcct cgtcgattat cccgagttca atctgcgggt
     3481 tgcggagcgc tgcaaagcca tgggcatccg cgtcatctat tacatttcgc cgcaggtctg
     3541 ggcctggcgg aagggacggg tgaagaagat cgccaggatc gtcgacctga tgatcgtcat
     3601 ctttccgttc gaggagacgt tttaccgcga gcacgatgtg ccggtcgtgt acgcaggcca
     3661 tccattgatc gacgaactgg ctggtgttgc acgcggtgat cacgagccgg gcacgccgct
     3721 gcgattggcg ctccttcccg gcagccggcg cggcgaggtc tcgcaactgc ttccagcgat
     3781 gctcgacgcg gttgccaatc ttcgccgcga gcgcgagctc gacgccttcg tgatccaggc
     3841 caccacgatc tcctcggccg agttgctcgc gatcatgcag acggccaacg tctacgtccg
     3901 catcgtgccg cacggcggcg gcgaagagct ggcgtcggcg gatgtcgcgc tctcgtcgtc
     3961 cggcaccgcc acgctcgaat gtgcggtcat cggtacgccg gtcatcgtca tgtaccggct
     4021 gtcgacggcg acgctctggt ttgcccgacg gctcgtgagc ctgcagcatt tcagcctcgt
     4081 caacatcctg gccgggaaga aggtcgtccc ggaactgttg cagcacgacg tgaacggtga
     4141 tcgcatcgca gcggaagtgc gcgcgctggc gcaacccgag aatcacgcgc gcgttcgagc
     4201 cgagctcact gcggttcgcg cactcctcgg caagggcggc gccagtcatc gggccgcaga
     4261 agcgataatg agcgcggtgc atcgatgacc gttcttcgcc ggctctaccg ctaccttcgc
     4321 cactacaagg catgggcggc gctcgccttc ggatccatga tcatcttcgc cgtcacgcaa
     4381 acggccatga tcggactcgt gcagccgatc ttcgacatcg gcctgacccc gccgtcgtcg
     4441 aagccatacg cggagaccca tcagtcaaag gaagagaagg tcaagagcgg catcctcgat
     4501 acagtcctcg agcgcgagaa gcctgaaggt caacgtggtt ggctcatcaa tcacggggac
     4561 gaactgaatc accagtttca cgggtggtgg gacaaagcca gccgcaagat tcagttccgg
     4621 cgccttctgc tggcgatgct gatcgtcatc atcctgcgga cctttgcctc gttcttctcc
     4681 gaatatgcgt tccagaaagt cgggctgtcg acggtgcggg acctccgcaa cgagttgtac
     4741 gagcgtctca tcttccagag tcatcgcttc ttctcggaac gatcgacagg ggagatggtc
     4801 agccgcgtgg tgtcggacgc cgacgccatt caggccgcag tgtcgacgcg catgggtgat
     4861 ctgttccagg agtcgctcac gctcttcttc ctgctcgtct acgtcttctg gtcgaacccc
     4921 gagcttgcgc tgatcacgtt catcgccgcg ccgctgctcg tctttccggt cgtccacttc
     4981 ggcaaacgtc tgcgaggcac gacgcaccgt tcgcaggagc ggatggcgga catggccact
     5041 ctgctcgagg agaccatccg cggcgtacgg atcgtcaagg ccttcacgat ggagcgtttc
     5101 gagatcagcc gtttccgcga cgccacgaag cggcatctct cgtcgaacct gaaggcgcag
     5161 aagatccagg cgctctcctc gccggtcatg gacttcatca ccggcatcgg cgggctgtct
     5221 ctcttcctgt atgcgcagcg ccgcatcggc aacggaacgc tgagcatcgg cgcgctcatg
     5281 gcctacatgg cggcggtggc ggcgatgtat cagccgatga agaagctgaa caaggtcaat
     5341 ctctccgtga acacggcgct ctcggccgcc gagcgcgtct tccggatgct cgacattccc
     5401 aacgaagtcg aagacaggcc gaaggcagag acgatttcgg ccgtgggcag tggcatctgc
     5461 tatgagaacg tgtccttcca atacgaggag gaacctgtcc tgcgcgacgt cggactcacg
     5521 gtcgcgccgg gcgagatcgt cgctctcgtt ggcggaagcg gcgccggcaa gtcgaccttc
     5581 ctgaatctcc tgccgcgctt ctacgacgtc aatgccgggc gcatcaccat cgacggtcac
     5641 gacatccgcg atctgcagct ttcctcgctg cgcggcctga tgggactggt gacgcaggag
     5701 gtcgtgctct tcaacgacac ggtgcgcaac aacatcgcct acggccgcgg ggacgtcgac
     5761 tcggagcgcg tgatcgatgc ggcgaaggct gccaacgcgc acgatttcat ctccgcgatg
     5821 ccgcaggg
//
LOCUS       DATLVW010000179         3074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_418780, whole genome shotgun sequence.
ACCESSION   DATLVW010000179 DATLVW010000000
VERSION     DATLVW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418780"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VNN25_05765"
     CDS             <1..324
                     /locus_tag="VNN25_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91067.1"
                     /translation="IVGAEHLIGGAEHLIGGAEHLIGSTEHSIGGAKHIIVVSGRRIV
                     AAKDIMVGAEHIIACANHSIAGERRSVESVKHWINGSKRALPQHGRHDANHSSNSQGG
                     CSRPS"
     gene            complement(463..1314)
                     /gene="panC"
                     /locus_tag="VNN25_05770"
     CDS             complement(463..1314)
                     /gene="panC"
                     /locus_tag="VNN25_05770"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXH91068.1"
                     /translation="MILATTIEATRAAVATARTSGRTIGFIPTMGFLHDGHLSLIDAV
                     RANGADFIVVSIFVNPTQFGPNEDFERYPRDEARDRDLLEGKDVDVLFLPSVSVMYPS
                     GSQTSVHTGDVARPLEGERRPGHFDGVATVVLKLFNIVQPDLAVFGRKDAQQCAVIER
                     MVRDLDVPVKLVFAETAREDDGLARSSRNSYLSADERAIAPALYRALRAGEEAISREI
                     HDTGAIESLMRKTIEESPGLTIDYLAVIDPETFLTPADFHRDILLAGAVKVGRTRLID
                     NVRIARA"
     gene            complement(1452..2333)
                     /gene="panB"
                     /locus_tag="VNN25_05775"
     CDS             complement(1452..2333)
                     /gene="panB"
                     /locus_tag="VNN25_05775"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXH91069.1"
                     /translation="MADTPKRITVPAIRAMKGEKRIGMITAYDYPSAKVADAAGSDII
                     LVGDSLGMVVLGYPDTLSVTVDDMLHHTRAAARGAAHALVVGDMPYLSYHVSVEESVR
                     NAGRFIQAGAQAVKVEGGKASRIRAIEAILDAEIPVMGHIGLTPQSVNALGGFKLQGK
                     NLDDARRLVDEALALDQAGCFSIVLECVPSELAAMITERVSIPTIGIGAGPSCDGQVL
                     VFHDLLGIYDGHRAKFVRRYANIADDMRAAIEHYLADVRDGSFPDDQKESFHAASADD
                     LRALYGEGVVIEMPKAQ"
     gene            complement(2460..>3074)
                     /locus_tag="VNN25_05780"
     CDS             complement(2460..>3074)
                     /locus_tag="VNN25_05780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXH91070.1"
                     /translation="AVEGPIGVGKTSLVNLLAKRFRGTKILEDVDNPFLADFYKDKRG
                     AAFRCQLFFLLSRYDQQRSISQRDLFTELILADYSFPKDKIFAYMTLEDSELMIYNRM
                     YELLYDSVPKPDLVIYLQASLDTLLKRVKKRGRAYEKSISQDYLKELSEAYSHYFYRY
                     DETPLLVVNTNEIDFVNTPEHFDQLIEQIRNAKKGTQYYVPLGS"
BASE COUNT          604 a          895 c          965 g          610 t
ORIGIN      
        1 atcgtgggcg cagaacatct catcgggggt gcagaacatc tcatcggggg tgcagaacat
       61 ctcatcggga gtacagaaca cagcatcggg ggtgcgaaac acatcatcgt ggtgtcagga
      121 cgcaggatcg tggctgcaaa agacatcatg gtgggtgcag agcacatcat cgcgtgtgcg
      181 aatcacagca tcgcgggtga aagacgcagc gtcgagagtg tgaaacattg gatcaatgga
      241 tcgaaacgcg ccctgccaca gcacggacgg cacgacgcaa accacagcag caacagccaa
      301 ggtggttgct cccgcccgtc ttagcttgtc atttctcaca gttgtaggag tgcgttgtca
      361 gttgattgcg gcgctgccgc gccccgttgt cgggagaccg aggcgacaat cccgcgcaac
      421 cgacaacgcg cgaagcgcaa tcaaccgaca acgcacgcgc cgctaggcgc gtgcaatccg
      481 cacgttgtcg atcaacctcg tccgcccgac cttcaccgcg ccggcgagga ggatgtcgcg
      541 gtgaaaatct gcgggcgtga ggaatgtctc gggatcaatg accgccagat agtcgatcgt
      601 caagccggga ctctcttcga tcgtcttccg catcagcgat tcgatcgcgc cagtgtcgtg
      661 aatctcccgg ctgatcgcct cttcgccggc gcgcagggct cgataaagtg ctggagcaat
      721 cgctcgctca tcggcggaga gatagctgtt ccgcgatgat cgcgccagac catcatcctc
      781 acgcgccgtc tcggcaaaga cgagcttcac ggggacgtcg aggtcgcgca ccatccgttc
      841 gatcaccgcg cattgctgcg cgtctttgcg gccaaagaca gcgaggtcgg gctgcacgat
      901 gttgaagagt ttcaagacga cggtggctac gccgtcgaag tgtcccgggc ggcgctcccc
      961 ttcaagtggc cgggcgacgt cgcccgtgtg taccgatgtc tgcgagcccg aggggtacat
     1021 gacgctgacc gaggggagga agagaacatc cacgtctttg ccttcaagta gatccctgtc
     1081 gcgggcttca tcgcgagggt agcgctcgaa atcctcattc gggccgaact gcgtcgggtt
     1141 gacgaagatc gacacgacga tgaagtctgc gccgttcgcg cgcacggcgt cgatgagcga
     1201 gagatggccg tcatgcagga agcccatcgt cgggatgaag ccgatggttc tgccggaggt
     1261 acgcgcggtt gcaacggcag cgcgggtggc ctcgatggtt gttgcgagga tcatcgatgc
     1321 tgtttagctt ctagagcaca gacaggagtg tctgtgccac accaagcccg gctccgaggt
     1381 gtgtttgtgc ccaaagccac gtggcacaga cactcttctc tgtgcttctc gatctgcaag
     1441 cggactcggt ctcattgcgc cttcggcatc tcgatcacga caccttcacc atatagcgct
     1501 cgcagatcat ccgcgcttgc cgcgtggaac gactccttct gatcatcggg gaaacttccg
     1561 tcgcgcacat ccgccaagta atgctcgatt gcggcgcgca tgtcgtcggc gatgtttgcg
     1621 tagcgccgca cgaacttcgc tcggtggccg tcgtagatgc cgaggaggtc gtggaagacg
     1681 aggacttgtc cgtcgcagga ggggccggcg ccgatgccga tggtcgggat cgaaacgcgc
     1741 tcggtgatca ttgctgccag ctccgacggc acgcactcaa gcacgatcga gaaacagccg
     1801 gcctgatcga gcgcgagcgc ctcatcgacc agtcgccgcg cgtcgtcgag attctttccc
     1861 tgcagtttga agccgccgag agcgttgacc gattgcggcg tgaggccgat gtggcccatc
     1921 accggaatct cggcgtcgag aatggcttcg attgcgcgga tgcgcgaggc tttgccgcct
     1981 tcgaccttga ccgcctgcgc gcccgcctgg atgaagcggc cggcgttgcg gacggactct
     2041 tccacgctca cgtgatacga gaggtagggc atatcgccga cgacgagcgc gtgcgcggcg
     2101 ccgcgtgcgg ccgcgcgtgt gtgatggagc atgtcgtcga ccgtgacgct cagcgtgtcg
     2161 ggataaccga gtaccaccat gccgagggaa tcgccgacca ggatgatgtc ggagccggcc
     2221 gcgtcggcga ccttcgcgct cggatagtca tacgcggtga tcatcccgat gcgcttctcg
     2281 cctttcatgg cgcggatggc cgggacggtg atgcgtttcg gcgtgtctgc catttgctcc
     2341 tccgtggtgg gccgggagga gcaagtagtc agagatcaga gatcagagat caggaaagct
     2401 gagtccctga tctctaatct ctgaccactg accactacgc ttcgtctcgg tccgtgtact
     2461 caggatccaa gcggtacgta gtactgcgtg cctttcttcg cattgcgaat ctgctcgatg
     2521 agctgatcga aatgctcggg cgtgttgacg aaatcgatct cgttcgtgtt cacaaccagc
     2581 aatggcgtct cgtcgtaccg gtaaaagtag tgtgagtacg cctcgctgag ctctttgagg
     2641 taatcctgcg agatcgactt ctcgtaagcc cgtccgcgct tcttcacgcg tttcaatagt
     2701 gtatcaaggg aagcctgaag atagataacc aggtccggct tcggcacgct gtcgtacagg
     2761 agctcgtaca tccggttgta gatcatcagc tccgagtctt cgagcgtcat gtacgcgaag
     2821 atcttgtctt tcggaaacga gtagtccgca aggatcagct cggtgaagag gtcgcgctgc
     2881 gagatcgatc gctgctggtc gtagcgcgag agcagaaaaa agagctggca gcggaaggcc
     2941 gcgccgcgct tgtctttgta aaaatcggcg aggaacgggt tgtcgacgtc ctcgaggatc
     3001 ttggtccccc gaaagcgctt agcaagcagg ttcaccaacg acgtcttccc gacgccgatc
     3061 ggtccctcga cggc
//
LOCUS       DATLVW010000180         6655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_52092, whole genome shotgun sequence.
ACCESSION   DATLVW010000180 DATLVW010000000
VERSION     DATLVW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52092"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VNN25_05785"
     CDS             complement(<1..1526)
                     /locus_tag="VNN25_05785"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXH91071.1"
                     /translation="MRSDVPAALPLIAYAAGLALGYSYAEAAGFAVVAVLLIALRRTR
                     MAFACLALAGGVWAAAHAREATRVDDAAIAPLVTDRFVTVVAPIDRDWSVRGEAHALR
                     CVRFVADGIAIERPLTIYVRFEPSEIGMERSIRAEGFLRRNERGDLALTIKSPRLMAY
                     EGKLNPMAPGAWNRALANRIRPFAGVHPTEVALVEAIALGRSERLTDEVRDSYKHGGT
                     YHLLVFSGLQIAFAAALIALALRWLRAPRTADWSLLIFSIIAPLFIGPTASVSRATIG
                     IGLYAISRILHRPTTLENLWCVAALLRLIIAPHDLVEPAFHLTYAGAGALLFAGKALA
                     ARRARWIAYAGAAEMSITPLTLFHFHQYALGGSVMTMLLTPIVFAMLVLSVAICAIPC
                     NALFAALGSLHQLCTFVNGVATPVWGFFAAPPLIALVTGYAIAILAVAFLRGRIRALA
                     ILAAMLIPIGGAIVVARQDVVAPRLTMLDVGQGDSLLLRSPKHVVLIDAGGRLGDAHF
                     G"
     gene            complement(1516..2631)
                     /locus_tag="VNN25_05790"
     CDS             complement(1516..2631)
                     /locus_tag="VNN25_05790"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXH91072.1"
                     /translation="MRVGDQLTIAPTELVAGGAALAKVDGFPIFIAGAYPGDVAVVRL
                     VEVKKGFAQADLVRLVEASPFRRAVPCPVTNECGGCDWTALRLDKQLEAKERILTESL
                     RRVGKIDLATLPPITVHPSPLNYRIRSRLHQDGGKVGFFASRTNRVVQLPAECEVVGP
                     ETARALSSGAGFTGSEAAEIQIWEVDGKLVTEGELTIDGFHVTTDVFFQVNRHLLGTL
                     RRLVADIASRSAKKETAVDLYSGVGFFSVPLAGVFANVTAVEGSASAHACAARNVPDN
                     VTLVNAPAEWWVERMARVDFVFLDPPRSGAKRNVIDAIGTRAGSMVCFLACDPVTFAR
                     DASRLIASGWRLASLDLLDLFPNTHHVETLASFERAQ"
     gene            2674..2889
                     /locus_tag="VNN25_05795"
     CDS             2674..2889
                     /locus_tag="VNN25_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH91073.1"
                     /translation="MRDLASILALPTEDRLRLIEAIWDSLTEVPEAVPVSDELKSKLD
                     RRLDSYHANRTAVRPWGEVRAELFDPK"
     gene            3249..3773
                     /locus_tag="VNN25_05800"
     CDS             3249..3773
                     /locus_tag="VNN25_05800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH91074.1"
                     /translation="MFGSRDQALQRAAGGDHESFATLIHEHQDMVFSIAWNFLHDRGR
                     AEEIAQEVFLQLYRTLGEIESPAHLTFWLRRVTSNRCIDESRRGRWRIVTLDEASDVA
                     HDADATDPLLKRKVGQMLQRLPAQQRLALTMRYQEGLEPSEIGAVLGMPLNTVKSHLR
                     RGLAALREWLGATS"
     gene            3770..4093
                     /locus_tag="VNN25_05805"
     CDS             3770..4093
                     /locus_tag="VNN25_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91075.1"
                     /translation="MNITDKNIEEALQQTLARTSAPEGFADRVLARIGDPQSQPQRSW
                     LSASALRRIAAAILFTAALGGLTAHQIAEHRRAEGELARREVMTALHIASAKMRVAQR
                     AVARD"
     gene            4151..4723
                     /locus_tag="VNN25_05810"
     CDS             4151..4723
                     /locus_tag="VNN25_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HXH91076.1"
                     /translation="MNATKLLLSLLFTIIASAAFPLAAQAQTPRINMDFPELASKARE
                     TVDVTLDGPMLRLAGRFLSSTDADERSAKEMINGLSGIYVRSYEFEHEGEYDKAIAER
                     IRTQLGSSWKKIVKVTSRTREDVDIYAEMRGDAITGLLIISAEPREFTVVNIIGPVDL
                     EKLTGLEGQFGIPHITRDMKSKHGKEEHHE"
     gene            4716..5201
                     /locus_tag="VNN25_05815"
     CDS             4716..5201
                     /locus_tag="VNN25_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91077.1"
                     /translation="MSNRRRILLFVALMSVLTLGATPAHAGFDDVVRAVESRYHVHHT
                     GIPFFGLIRFALWVAQPDGVSDLQLATFEHTHIDDERGLAEIVTRNAGEEFRPLVRTR
                     SSRSGEVTQILAHPMGRDRVALLIFAHERAETTIVRVVVSMDKFEEAMNKPDSVMASI
                     R"
     gene            5321..6343
                     /locus_tag="VNN25_05820"
     CDS             5321..6343
                     /locus_tag="VNN25_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009487257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HXH91078.1"
                     /translation="MTFLIYGANGYTGELIAREAVRRGLRPIVAGRSAEKIDLLASEL
                     GLEGRAFPLTEPKLDGVDAVLHCAGPFVHTSAPMVRACLDAGIHYLDITGEIPVFEAI
                     MTMNDVAVQRGVTLIPGVGFDVVPTDCLAAMLAAKLPGATELELAFYTPKAELSRGTL
                     KTMIESIGEGGAIRRDGRIVRVPPAYDVRTIPFSCGPRTAMTIPWGDVSTAFHTTGIP
                     NIRVYTGASPKSVARMRRIRSFLPLLGIKPVKRLLQSFANRRSGPGEAQRAMGRTYLW
                     GRAANGVDEVTMTMTTPEGYLFTVLSALAAVERLLASPRRPGSFTPAKYFGAEFVTTI
                     VGVELT"
     gene            complement(6492..>6655)
                     /locus_tag="VNN25_05825"
     CDS             complement(6492..>6655)
                     /locus_tag="VNN25_05825"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876496.1"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXH91079.1"
                     /translation="HITVPAGLAFGLPVGLSIFGGAWSDPSLIKYAYAFEQAAKARQR
                     PKFLSATSA"
BASE COUNT         1324 a         2199 c         2008 g         1124 t
ORIGIN      
        1 ccgaaatgcg catcgccaag ccgtccgcct gcgtcgatca ggacgacgtg cttgggtgag
       61 cgaaggagca gcgaatctcc ctgaccgacg tcgagcatcg tcaaacgagg cgcaacgaca
      121 tcctgtcgcg cgacaacgat ggcaccgcca atcggaatca gcatcgcagc cagaatcgcg
      181 agtgcgcgta tccggcctcg caggaacgcg accgcgagga tcgcaatcgc atagccggtt
      241 accagcgcga tcagtggcgg tgcggcgaag aagccccata ccggcgtggc cactccattg
      301 acgaacgtgc agagctggtg cagagacccc agcgccgcga agagcgcgtt gcagggaatc
      361 gcgcaaatcg ccaccgacag cacgagcatc gcgaaaacga tcggcgtcag cagcatcgtc
      421 atcaccgatc cgccgagcgc gtactggtga aagtggaaga gcgtcagcgg cgtaatcgac
      481 atctccgccg ctcctgcgta ggcgatccag cgcgcgcgcc gtgcggccag cgctttgccc
      541 gcaaagagca gcgcacccgc tcccgcgtac gtcaggtgaa aagccggctc gacgagatcg
      601 tggggcgcga tgatgagacg caacagcgcc gccacgcacc agagattctc cagcgttgtc
      661 ggacgatgca gaatccgtga gattgcgtag agaccgatgc cgatcgtggc gcgcgacacc
      721 gaagccgtcg gcccgatgaa gagtggcgcg atgatcgaga agatcagcag cgaccagtcc
      781 gccgtccgcg gcgcgcgcaa ccagcgcagc gccagcgcga tcagtgccgc cgcgaacgcg
      841 atctgcaatc ccgagaaaac gagcaggtga tacgtcccgc catgtttgta gctgtcgcgc
      901 acctcatcgg tgagccgttc gcttcttccc agtgcaatcg cctcgacgag cgcaacctcg
      961 gtcggatgca caccggcgaa cgggcggatc cgattcgcta gcgcacggtt ccacgcgccc
     1021 ggcgccatcg gattcaactt cccttcgtac gccatcaacc tcggcgactt gatcgtcagc
     1081 gcgaggtcgc cccgctcatt gcgccgcaga aagccttcgg cccggatcga gcgctccatg
     1141 ccgatttccg acggctcgaa ccgcacataa atcgtcagcg gccgttcgat cgcgatgccg
     1201 tccgccacga agcgcacgca acgcaaggca tgcgcctcgc cgcgcaccga ccagtcgcga
     1261 tcgatcggag cgacgacggt gacgaaccta tccgtgacga gcggtgcgat cgcggcgtca
     1321 tcgacacgcg tcgcttcacg cgcatgcgcg gcagcccaga cgccgcccgc gagcgcaaga
     1381 caagcgaacg ccatccgcgt cctacgaagc gcgatgagca acaccgccac aaccgcgaat
     1441 ccggcagcct cggcgtacga atacccgagg gccagaccgg cggcgtaagc gatcagcgga
     1501 agtgcggcgg gaacgtcact gcgcacgctc gaacgaagcg agcgtttcca cgtgatgcgt
     1561 attcgggaag aggtccagga ggtccagcga cgcaagccgc cagccagacg cgatcagccg
     1621 gctcgcgtcc cgtgcaaacg ttaccggatc gcaggcaagg aagcaaacca tcgagcccgc
     1681 gcgcgtgccg atggcatcga tgacattacg cttggcgccg ctgcgaggcg gatcgagaaa
     1741 gacaaagtcg acgcgcgcca tccgctctac ccaccactcc gccggcgcat tcacgagcgt
     1801 cacgttgtcc ggcacattgc gcgccgcgca agcgtgcgcc gaggccgatc cctcgaccgc
     1861 cgtgacgttc gcgaacacgc ctgcgagcgg cacgctgaag aaaccgacac ctgagtagag
     1921 gtcgaccgcg gtttccttct tcgccgaccg cgatgcgatg tccgcgacca gccgccgcag
     1981 cgtgccgagg agatgccggt tgacctggaa gaaaacatcg gtcgtaacgt ggaacccatc
     2041 gatcgtcagc tccccctcgg tgacaagctt tccatcgact tcccagatct gaatctccgc
     2101 tgcctcacta cctgtaaaac ccgcacccga cgaaagtgcg cgcgccgtct ccggccccac
     2161 cacttcgcac tccgccggaa gctgcacgac gcgattcgtc cgcgatgcga agaacccgac
     2221 cttgccgccg tcctgatgca gccggctacg aatccggtaa ttgagcggcg acggatgcac
     2281 cgtgatcggc ggaagcgtgg ccagatcgat cttgccgacc cgccgtaacg actccgtcaa
     2341 aatccgctcc ttcgcctcga gctgcttatc gagccgaagt gccgtccaat cgcacccgcc
     2401 acattcattc gtcaccggac agggaaccgc gcgccgaaat ggactcgcct cgaccaatcg
     2461 cacgagatcc gcctgcgcaa accccttctt cacctcgacc agccgcacga ccgcgacgtc
     2521 gccgggatag gcgcccgcga tgaagatcgg aaacccatca accttcgcca gcgccgcgcc
     2581 gccggcgacg agttcggtcg gggcaatcgt cagctgatca ccaactcgca ttgacggcga
     2641 ggatagcgcg gtcccctcgg atagacttac tcaatgcgtg acttggccag cattcttgcg
     2701 ctgccaacgg aggaccgcct tcgtctcatc gaagcgattt gggactcact caccgaggtt
     2761 cccgaagcag tcccagtcag cgatgaactg aagtcgaagc tcgatcgccg ccttgattcg
     2821 taccatgcga accggaccgc ggttcgtccc tggggcgaag tccgcgccga gttgttcgac
     2881 cccaagtaag cgcctctcgc gcgccaggga gcgacccttc tacttagcag ccgagcactc
     2941 tgcgcgcaag aaggagaggt gtcatcctga gacgccggag ccgccatccg tacttgaggc
     3001 gtcgcgctgc aagatgtggc ggcgcaggac gtcgaaggat ctcaaattac gcgacgctgt
     3061 catgtgcgcg gcaacttgag atccttcgcc gtcctgcggc gccagggaag atctgtgtga
     3121 aagcaacgag tggcgcgggc gcctccggca ggctcagaat gacacctaaa ttcctttatc
     3181 gatgctgatt gcattcgttc gcccgattcc gccgtatata gcctcagatg actgctacgg
     3241 cgccattgat gttcgggtcc cgggaccagg ctctgcaacg ggcggcgggc ggggatcatg
     3301 agtcgttcgc gacgctcatc catgaacatc aggacatggt cttcagcatc gcctggaact
     3361 ttcttcacga ccgggggcgt gccgaggaga tcgcgcagga ggtattcctg cagctctatc
     3421 gcacgctcgg cgagatcgaa tcgccggcgc acctgacctt ctggctgcgc cgcgtgactt
     3481 ccaaccgctg catcgatgaa tcgcggcgtg gccgctggcg catcgtcaca ctcgacgaag
     3541 catctgatgt tgcgcacgac gccgatgcga cggacccgct actcaagaga aaagtcggtc
     3601 agatgctgca gcgtctgccc gcgcaacagc gcctcgcgct gaccatgcgt taccaggagg
     3661 gactcgagcc ttccgagatc ggagcggtgc tggggatgcc gctcaacaca gtcaagagcc
     3721 atcttcgccg cgggctggcc gcgttgcgcg aatggctggg agccacgtca tgaacatcac
     3781 ggacaagaac atcgaggaag cgctgcaaca aactctggcg cggacctcag cacccgaggg
     3841 cttcgcggat cgcgtgctcg ctcgtatcgg cgatccgcaa tcgcagccgc aacggagctg
     3901 gctgtccgca tcggcactcc gccgcatcgc tgccgccatc ctcttcaccg cagccctcgg
     3961 cggacttacc gcgcatcaga tcgccgaaca ccgccgcgcc gagggagagc tcgcccgacg
     4021 tgaggtgatg accgcactgc atatcgccag cgccaagatg cgcgtcgcgc aacgcgcggt
     4081 ggcgcgggat tgaaaccaga gatccgtcgg atcagtcaga tcaaaactaa ccaagagagg
     4141 cacacacacc atgaacgcaa ccaaacttct actcagctta cttttcacca tcatcgccag
     4201 cgcggcgttt cctctcgccg cccaggcgca gacgccgcgc atcaacatgg actttcccga
     4261 gctcgcctcc aaggcccgcg agacggtcga tgtcacgctc gacggaccga tgcttcgcct
     4321 ggccggcagg tttctctcca gcaccgacgc cgatgagcgc tcggcgaagg agatgatcaa
     4381 cggcctctcc ggcatctacg tccgcagcta cgagttcgag cacgaagggg agtacgacaa
     4441 ggcaattgcg gagcggatcc gcacacagct cggctcctcg tggaagaaga tcgtcaaggt
     4501 caccagcagg acgagagagg atgtcgatat ctacgcggag atgcgcggcg acgccatcac
     4561 cggacttctc atcatctcgg cagaacccag agagttcacc gtcgtaaaca tcatcggtcc
     4621 ggttgatctc gagaaactga ccgggctcga aggacagttc ggtatcccgc acatcacgcg
     4681 cgacatgaag tccaagcatg gcaaggaaga gcaccatgag taatcgccgc cgcatccttc
     4741 tgttcgtcgc gctgatgagc gtcctcaccc tcggcgcgac accagcgcac gcaggtttcg
     4801 acgatgtcgt ccgtgccgtc gagtcccgct accacgttca tcacacgggg attccgttct
     4861 tcggcctgat tcgtttcgcg ctctgggtcg cgcagcccga tggagtttcc gatctgcaat
     4921 tggcgacctt cgagcacacg cacatcgacg acgaacgtgg cctggccgag atcgttacgc
     4981 gtaatgccgg cgaggagttc cgcccgctgg tccgcacgcg atcgagccgc agcggcgagg
     5041 tgacgcagat cctcgcgcat ccaatgggcc gcgaccgcgt cgccctgctg atctttgccc
     5101 acgagcgcgc ggaaacgacg atcgtccgcg tggtcgtgtc gatggataag ttcgaagaag
     5161 cgatgaacaa gccggacagc gtgatggcga gtatccgata attgcgggcg cctggcggcg
     5221 cccgcgttgt cggttgtcgg ttgattgcgc ttcgagcgtt gtcgattttc taacttcgcg
     5281 taattcgcgt cttcgcgtga gaaactgtca aagcgcagca atgactttcc tcatctacgg
     5341 tgcgaatggc tacaccggcg agttgatagc tcgggaggcg gtgcgccgcg gattgcggcc
     5401 gatcgttgca ggtaggagtg cggagaaaat cgacctgctc gcgagcgagc tcggcctcga
     5461 agggcgcgcg tttccgttaa cagagccgaa gctcgacggc gtcgatgctg ttcttcattg
     5521 cgcggggccg ttcgtccaca ccagcgcgcc gatggtgcgg gcctgtctcg atgccggcat
     5581 tcactacctc gacatcacgg gtgagatccc tgtcttcgaa gcgatcatga ccatgaacga
     5641 tgtcgcggtt cagcgaggcg ttacgctcat ccccggcgtg ggcttcgacg tcgttcccac
     5701 cgattgcctg gccgccatgc tcgcggcgaa acttcccgga gcgaccgagt tggagctcgc
     5761 tttctacacg ccgaaagcgg agctgagccg cggcacgctg aagacgatga tcgagtcgat
     5821 cggtgaagga ggcgccatcc gccgcgatgg caggatcgtg cgcgttccgc ctgcgtacga
     5881 cgtgcgcacg attcccttct cctgcggacc gcgcacggcc atgaccattc cgtggggcga
     5941 tgtctccacg gcgtttcaca ccaccggcat cccgaacatt cgtgtctaca cgggcgcgtc
     6001 gcccaagtcg gtcgcgcgca tgcggcgcat ccgttcgttt ctgccactcc tcggaatcaa
     6061 gccggtcaag cggctcttgc agtcgttcgc taatcgccgc agcggaccgg gtgaagcgca
     6121 gcgcgcgatg ggacgaacct atctctgggg ccgcgcggca aacggcgtcg acgaagtcac
     6181 gatgacgatg acgactcccg aaggctattt gttcaccgtg ctgagcgcgc tggcggcagt
     6241 ggagcggttg cttgcatcgc cgcggcggcc gggcagcttc acccccgcga aatacttcgg
     6301 cgcggaattc gtgaccacga tcgtaggtgt tgaactgacg tgagacgcaa actcctcccc
     6361 ttctacgccg ccttcatcgc cttgatcgcg atctcattcg cctggcagat gctgcacggg
     6421 atttgtcccg tcccctgatt actgcatctg cgaacatctg cgtcatctgc ggaccggttt
     6481 ttatccgcgg attacgcaga tgtagcagat aggaacttcg gacgctgacg cgcctttgcc
     6541 gcctgctcga acgcgtacgc gtacttgatc agcgagggat cgctccacgc gccgccgaag
     6601 atcgacaggc cgaccggtaa gccgaaggcc agtccggccg gaaccgtgat gtgcg
//
LOCUS       DATLVW010000181         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_503202, whole genome shotgun sequence.
ACCESSION   DATLVW010000181 DATLVW010000000
VERSION     DATLVW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503202"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VNN25_05830"
     CDS             complement(<1..341)
                     /locus_tag="VNN25_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91080.1"
                     /translation="MQRFVFRPAALTALLIAIGAIRMVTTFRVFSVTNDEPTHVGAGL
                     ELLEYHRYNLQSENPPLARVIFAAIPELGGMRFNPKGNFTDQIHSVFYGHGEYKANLF
                     RARAGTLVFFV"
     gene            complement(371..1969)
                     /locus_tag="VNN25_05835"
     CDS             complement(371..1969)
                     /locus_tag="VNN25_05835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH91081.1"
                     /translation="MQRFALRPAALAALLIAIGAIRMATTFRVFSATSDEATHIGAGL
                     EMYQYHRYQFQRENPPLPRLVMAIAPYLGGMRYDDRGNFPEQIHSIFYGHGEYRANLV
                     RARVGNLLFFVIAAVVLFALARDAIGDKAALLSLLLFTMEPIVLGYSAVATHDGPGTA
                     GVAVALFAFTRWLRGPNLKSALLFGAAYGFGILCKFSSIPFVAVACAALVIVRLFHDA
                     ELRGHLGRAAVTLVPAAATTLLVIWAGYGFRVGTVSELQPWAPVFSPLVQRLLAQIHP
                     RTPLPASNFFIGIAGLLKYDQRGYLTYLCGKILKNGRWWYFPFAIALKTTIAALILFA
                     TGFWFVLRDREMRWPFAEWSAAAIAMILVAMPSALDLGARYLLPFYVPFTIAAACTVM
                     AMIRASRATAVIAVVLLVAHIGASALAHPDYFPYFNAFGGSDPSRYLVDSNIDWGQDI
                     LRLRTVARREHMGKLTASLMGPADYVSLGFPPIEPASPWTESHGWVAVSDHSYQLTGA
                     EGGWRWLPERYERVGKSIRLYRIP"
     gene            2029..>2314
                     /locus_tag="VNN25_05840"
     CDS             2029..>2314
                     /locus_tag="VNN25_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91082.1"
                     /translation="MRNRRHQILALVLIVCCIPLLSNGCKTKEQPAETNTLGNATDRN
                     PATGTNGTGEPVSPAVGTATAAQQLGTVATTTASGTSPVLTGTEEVHAQKS"
BASE COUNT          476 a          701 c          772 g          365 t
ORIGIN      
        1 atgacgaaga agacgagcgt gccggcgcgc gcgcgaaaga gattggcttt gtactcgccg
       61 tgtccgtaga agacgctgtg aatctgatcg gtgaagtttc ctttcggatt gaaacgcatc
      121 cctccgagct cggggatggc cgcaaagatg acgcgcgcca gcggcgggtt ctcgctctgc
      181 aggttgtagc ggtgatactc gagcagttcc aatcccgcac cgacatgcgt gggctcgtcg
      241 ttggttaccg agaagacgcg aaacgtggtg accatgcgga tcgcaccgat ggcgatcagt
      301 aacgcggtaa gagcggcggg acgaaacacg aagcgctgca tcaggggcgg cgattctact
      361 gcgcggtggt ttacggaatc cgatacaagc gtatcgactt tcccacccgc tcataccttt
      421 ccggcagcca ccgccacccg ccctcggcgc ccgtgagttg gtatgagtgg tcactgacag
      481 caacccagcc gtgcgattcc gtccatgggc tggccggttc gatgggcgga aagccgaggg
      541 acacgtaatc ggccgggccc atcaacgatg ctgtgagctt tcccatgtgc tcgcgccgcg
      601 caacagtgcg gaggcggagg atgtcctggc cccagtcgat gttgctatcg acgaggtagc
      661 gcgacgggtc ggagccgccg aaggcgttga agtatgggaa gtagtcggga tgagcgagtg
      721 ccgaggcgcc gatgtgggcg acgaggagca cgacggcaat gaccgcagtc gcgcgcgacg
      781 cgcgaatcat cgccatcact gtgcacgccg cggcgatcgt gaaggggacg tagaacggga
      841 ggaggtagcg tgcgccgagg tcgagggcgc tcggcatcgc cacgaggatc atggcgattg
      901 cggcggcgct ccactccgca aacggccatc gcatttcgcg gtcgcgcagc acgaaccaga
      961 agccggtcgc aaacaagatg agcgcagcga tcgtcgtctt cagtgcgatg gcgaacggga
     1021 agtaccacca tcgtccgttc ttcaggatct tgccgcacag gtaggtgagg tagccgcgct
     1081 ggtcgtactt caggaggccc gcgatgccga tgaagaagtt cgacgccggc agcggcgtgc
     1141 gtgggtggat ctgcgcaaga aggcgctgca caagaggact gaagacggga gcccatggct
     1201 gcagttcgct gaccgtcccg acgcggaaac cgtagccggc ccagatcacg agcagcgttg
     1261 ttgccgctgc cggaacgaga gtgactgctg cgcggccgag gtgaccgcgc agctctgcgt
     1321 cgtgaaatag gcggacgatg acgagagcgg cgcaagcgac tgctacgaat gggatgctgg
     1381 agaacttgca gaggatgccg aaaccgtagg cagcgccgaa cagcaatgct gacttgaggt
     1441 tgggcccacg gagccagcgt gtgaacgcga agagcgccac ggccacgccc gctgttccgg
     1501 ggccatcgtg tgtggccact gccgagtagc cgagaacgat cggctccatc gtgaagagaa
     1561 ggagagacaa caacgccgcc ttgtcaccga tcgcgtcgcg cgccagtgcg aacaaaacga
     1621 cagcggcgat cacgaagaag aggaggttcc ccacacgagc ccggacgagg ttcgcgcgat
     1681 actcgccgtg accatagaag atgctgtgga tctgttccgg aaagttcccg cggtcgtcgt
     1741 aacgcattcc acccagatac ggcgcgatgg ccatcacgag gcgcggcagc ggcgggttct
     1801 cgcgctgaaa ctgatagcgg tggtactggt acatctccag ccccgcgccg atgtgcgtgg
     1861 cctcatcgct cgtcgcggag aagactcgga acgtcgtcgc catccggatc gcgccgatgg
     1921 cgatcagcag cgcagcgagt gcggccggac ggagtgcaaa gcgctgcatg gcgtccggcg
     1981 attctactgc gcacgactct tgcctctctt tcgcggcgga ggcaaatcat gcgtaatcgc
     2041 cgtcatcaga tcctcgccct cgtgctcatc gtttgctgca tcccgctcct gtcgaacggc
     2101 tgcaaaacga aggagcagcc cgcggagacc aatacgctgg gcaacgccac ggaccgcaat
     2161 cctgccaccg gaacgaacgg cacgggcgag ccggtctcgc ccgcagtggg caccgccacc
     2221 gccgcacagc aactcggcac cgtcgccact accaccgcct ccggaacctc acctgtcctc
     2281 accggcacgg aagaagtgca cgcgcagaag tccg
//
LOCUS       DATLVW010000182         4954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_52216, whole genome shotgun sequence.
ACCESSION   DATLVW010000182 DATLVW010000000
VERSION     DATLVW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52216"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNN25_05845"
     CDS             complement(<1..216)
                     /locus_tag="VNN25_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91083.1"
                     /translation="MVLRPRIVLAALLLFAACGTYVPRYAFHAAQENQAHIDKVRIGQ
                     TLAEVQRIMGKAPERRNTHVRFDGISIE"
     gene            complement(207..1400)
                     /locus_tag="VNN25_05850"
     CDS             complement(207..1400)
                     /locus_tag="VNN25_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91084.1"
                     /translation="MRWTRMLSLVALCLVALSLHAQCGVERWTIKTGTDSQAPSINLG
                     TYISTTIYNMRSSTAPATLPATTRIAPRELNQYQITATLTKYAREGDSDYHLVIQDSS
                     ARTMIIEIPSPNCVGAGSPFGPGIANARRQFDARFTATSTFKTTSTPVTIKGIGFWDF
                     LHGQTGVAPNGIEVHPVLNITFGAAVVGTNPSILSAEAPVDPSDDVAYPADMIRDEDG
                     GRVRVFRGGDALGGALFHGGAVVEDPSIHVVFVGNGWNDESKRAILQKAHAINRDAGF
                     EGMAKYGVRTFGMRVSSTQIADAGKDLGDLDVQRLLANAVESGRIQHVDENTVYLVAL
                     APDVDASVAASRDWLSYHSEFHPTELPMRYVVVRTGLEPGRVSEAINASVYRALANPA
                     GNGWF"
     gene            1542..3188
                     /locus_tag="VNN25_05855"
     CDS             1542..3188
                     /locus_tag="VNN25_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91085.1"
                     /translation="MVLFFAGHAAILALLLVTAAGAGSLAVGAGEGLALRAALGLALC
                     AHACFFLAAVGQLRPLPLVVLTIVAIAGGAVRVRGIARPSASHVVAGLLVIAPLFVFA
                     LYPPIAFDETLYHLPFVRALAHDGALRFLPAMRFPVFPQLHELLCVPVFLLGGDVATH
                     LVALAEVIVTAALLIEWAGRYDVRAGWLAAALFLGSPLVISLATITYVDAALALFVAA
                     GFYAFDRERFLLAGFFLGTACSVKYLGWYFVLAALIIVLVRTAGRRREAGMFLIACAA
                     AALPTTAWIVMKTHDPFFPFASTSLWSLAPSRTVSLGERFVGALRVMWDVTFARGRMN
                     HQPPVTPLLIGMVAVVIAAAIRDTRARWVALLCAAYMAVFEFLPQDSRYLVPLLPLLS
                     VVPAVAVARRWGKAATLLALLAVAPGVAYAGYRIALLGAPPVTASERSAWLSQQVPPY
                     RALIHAGNERVYVCGGEQLKDYAGGELPGDFNGPFSYERILCDAHDTASIAQRLRPID
                     AHYFLVVKQTCQPLLPNGGMILAYEDAAAQLWRVQPASER"
     gene            complement(3167..3895)
                     /locus_tag="VNN25_05860"
     CDS             complement(3167..3895)
                     /locus_tag="VNN25_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91086.1"
                     /translation="MNAIELKGVSVRYRAEKVRSLKELVVRGLRPSRPFFYSLRNVTL
                     AIANGESVGIIGSNGAGKSTLLRVAAGIIQPSEGVAIGRGSIVPLMELGTGFEAELSG
                     RENILFNGALLGRTRAQMRAQMDAIIEFSGIEPFVDAPIRTYSTGMIARLAFSIATAV
                     DAETILLDEILAVGDAAFRERCDERIRTFVREGRTVVLVSHDTEAVMTLCRRTLWLSH
                     GEIAGDGRSEDVIREYEAHRSDAG"
     gene            complement(3895..4722)
                     /locus_tag="VNN25_05865"
     CDS             complement(3895..4722)
                     /locus_tag="VNN25_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91087.1"
                     /translation="MIVRDQERHNVAGASLRAWDEIHDLAAHANLLIELVHRDLTVRY
                     KRSIFGFLWTLLHPLLLMAIFTVVFSHLFRRETPHYETYFLSAYIAWNFFSQTVTNAM
                     ASVAWNGPLMKRVRVPRSIFTLSSIVSGLVNLVLSMLALLVIMLIVRAPLFPSLLFVP
                     ISLLIVGIFTLGISLALTALSVFFADVREMVQAGLPALLYMTPVIYPVSIVPASFRWI
                     VEWNPLTYLIEIVRAPIYYGHLPEPRTIIAALTISLISLLCGWLIFRRLAPSFEGRL"
BASE COUNT          854 a         1523 c         1614 g          963 t
ORIGIN      
        1 ctcgattgag atcccgtcga agcggacgtg cgtgtttctt cgctccggag ctttccccat
       61 gatgcgctgg acctcggcga gggtttgtcc gatgcgcacc ttgtcgatgt gcgcctggtt
      121 ctcctgcgcg gcgtgaaacg cgtagcgtgg gacgtacgtg ccgcacgccg cgaacaggag
      181 aagcgctgcc agaacgattc gcggtctcag aaccaaccgt tgcccgccgg attcgcgagt
      241 gcacggtaga cgctcgcgtt gatcgcctcg ctcacgcggc caggctcgag accggtgcgc
      301 acgacgacgt agcgcatcgg cagctcggtc ggatgaaact cggagtgata agaaagccag
      361 tcgcggctcg ctgccaccga cgcatcgacg tcaggagcga gtgcgacgag gtagacggtg
      421 ttctcatcga catgctggat gcgtcctgac tcgaccgcat tcgcgagcag gcgctgtacg
      481 tcgagatcgc cgaggtcctt tccggcgtcc gcgatttggg tcgagctcac gcgcatgccg
      541 aacgtgcgca cgccgtattt cgccattcct tcgaaccccg catcgcggtt gatggcatgt
      601 gccttctgca ggattgctcg cttcgactcg tcgttccagc cgttgccgac gaacacgacg
      661 tgaatggacg gatcttcgac gactgcgccg ccatggaaga gcgcaccgcc gagggcgtcg
      721 ccgccgcgga agacgcgcac gcggccgccg tcctcgtcgc gaatcatgtc ggccggatag
      781 gccacatcgt cagagggatc gaccggcgct tccgcggaaa ggattgaagg attggtgccg
      841 acgaccgccg cgccgaacgt gatgttgagg acgggatgga cctcgattcc gttcggcgcc
      901 acgcccgtct ggccgtgaag gaagtcccag aatccgatgc ctttgatcgt gacgggcgtc
      961 gaagtcgtct tgaaggtcga cgtcgccgtg aagcgggcgt cgaactgccg ccgcgcattt
     1021 gcgattcccg gaccgaaagg actaccggcg ccgacgcagt tgggcgatgg aatctcgatg
     1081 atcatcgtgc gtgccgatga atcctgaatc acgaggtgat agtcggagtc accttcccgc
     1141 gcatatttcg tgagcgtcgc cgtgatctgg tactgattca gctcgcgtgg cgcgatgcgc
     1201 gtggtggccg gcagcgtggc cggcgcggtc gacgagcgca tgttgtagat cgtggtggag
     1261 atgtaggtgc cgaggttgat gcttggggct tgggagtcgg tgccggtttt gatcgtccag
     1321 cgttccacgc cgcactgggc atgaagggag agtgctacga gacagagcgc caccagggac
     1381 agcatccggg tccatcgcat tcgatatctc cttcttgtca acgagagcgc agcgattgtc
     1441 gccgaagtgg atgcgcattg atacaaatga atttgaagga cagaattcat ccttgggtct
     1501 acgtcccttt tctctgcgcg ttccctcatc atgcgcaccg catggttctc ttcttcgcag
     1561 gccacgcagc catcctggcg ttgctgctgg tcacggccgc gggagcggga tcgctggcgg
     1621 tgggagcagg cgagggtctg gcgttgcgcg ccgctctcgg gctcgcgctt tgcgcccatg
     1681 cttgcttttt tctcgccgcg gtcggacagc ttcggcctct accgctggtc gtgctcacga
     1741 tcgtcgcgat cgccggcggc gccgtgcgcg ttcgcggaat cgcgcggcca tcggcatcgc
     1801 atgtagtcgc cggtctgctc gtgatcgctc cgctgttcgt gttcgcgctc tacccgccga
     1861 tcgcgttcga tgagacgctc taccacctgc cgtttgtgcg cgcgttggcg catgacggcg
     1921 cgcttcgatt tctgccagcg atgcggttcc cggtcttccc gcagctccac gagctcctct
     1981 gcgttcccgt ctttctcctc ggcggcgatg tggccacgca tcttgttgct ctcgctgagg
     2041 tgattgtcac tgcggcgctg ctcatcgagt gggccgggcg atacgacgtt cgcgcaggct
     2101 ggctggcagc ggcgcttttc ctcggcagcc cgctcgtcat cagtctggcg acgatcacgt
     2161 acgtcgatgc ggctctggcg ctgttcgttg ctgcggggtt ctacgcgttc gatcgcgagc
     2221 gattccttct cgccggtttc ttcctcggaa ccgcgtgcag cgtgaaatac ctcggctggt
     2281 actttgtgct ggccgcgctg atcatcgttc tggtgcgcac ggctgggaga cggcgcgagg
     2341 cgggcatgtt cttgatcgcc tgcgcggcag cggcgttgcc gaccaccgca tggattgtga
     2401 tgaagacgca cgatccattc tttccgttcg ccagcacgag cctctggtcg ctcgcgccct
     2461 cgcgaaccgt ctcgttgggt gaacgcttcg ttggcgcgtt gcgcgtgatg tgggatgtaa
     2521 ccttcgcgcg cggacggatg aaccatcagc cgccggtcac tcctcttctc atcggaatgg
     2581 tggctgtcgt catcgcggcc gcgattcgcg acacgcgcgc gcgatgggtt gcgctccttt
     2641 gcgccgctta catggcggtc ttcgagtttc tgccgcaaga ctcgcgctac ctcgtgccgc
     2701 tgctgcctct tctcagcgtc gtgccggcgg tggccgtggc cagacgatgg ggaaaggcgg
     2761 cgacgctgct cgcgttgctc gcggtcgcgc ccggagtcgc ctacgcggga taccggatcg
     2821 cgttgttggg agctccgccg gttaccgcct cagagcgcag cgcgtggctg tcgcagcagg
     2881 tcccgccgta tcgcgcactc attcacgccg gcaacgagcg cgtctatgtt tgcggcggcg
     2941 agcagttgaa ggactacgcc ggcggcgagc tgcccggcga cttcaacgga ccgttttcgt
     3001 acgagcgcat tctgtgcgac gctcacgaca ccgcatcgat cgcgcagcgg ctacggccca
     3061 tcgacgccca ctactttctc gtcgtcaagc agacttgtca gccgctgctg ccgaacggcg
     3121 gaatgatcct cgcttacgag gacgccgcgg cgcagctctg gcgcgttcag cccgcatccg
     3181 agcggtgagc ttcatactcc cgaatcacat cctcggaacg gccatcgccg gcgatctcgc
     3241 cgtgcgatag ccagagcgtg cggcggcaaa gcgtcatcac cgcctcggtg tcgtgcgaga
     3301 caagaacgac cgttcttccc tcgcgaacga acgtccggat acgctcgtcg cagcgctcgc
     3361 gaaaggcggc gtcgccgacc gcgagaatct cgtcaagaag gatcgtctcc gcatcgaccg
     3421 cggtcgcgat cgagaacgcc agtcgcgcaa tcatgccggt ggagtacgtt cggataggtg
     3481 cgtcgacgaa cggctcgatg cccgagaatt cgatgatcgc gtccatctgc gctcgcatct
     3541 gtgcgcgcgt gcgtcccaga agagcgccgt tgaagagaat gttttcgcgg ccggacagct
     3601 cggcctcgaa gccagtgccg agctccatca gcggcacgat gctgccgcgg ccgatggcca
     3661 cgccttcgga aggttgaatg attccggcgg ccacgcgcag cagcgtcgac ttaccggcgc
     3721 cattcgaccc gatgattccg accgactcgc cgttggcgat ggcaagcgtg acgtttcgca
     3781 gactgtaaaa gaatggccgg ctaggacgca gcccgcgcac gacgagctcc ttgaggctgc
     3841 ggaccttttc cgctcgatag cggacgctca cgcctttcag ttcgatcgcg ttcatcagag
     3901 ccttccctcg aagctcggcg cgagccgccg gaagatcagc cagccgcaca gcagcgagat
     3961 cagcgagatc gtgagtgccg cgatgatcgt tcgcggctcc ggcaagtgcc cgtagtagat
     4021 cggcgcacgc acgatctcga tgaggtaggt gagcggattc cactccacga tccatcgaaa
     4081 cgaagccggc acgatcgaga cggggtagat caccggcgtc atgtacagaa gcgccggaag
     4141 gccggcctgc accatctctc ggacgtcggc gaaaaagacg gacaaggccg tcagcgcgag
     4201 tgagatgccg agggtgaaga tgccgacgat gaggagactg atcgggacga acagcagcga
     4261 cggaaacagc ggcgcgcgca cgatcaacat gatgaccagc agcgccaaca tcgaaagcac
     4321 caggttcacg agaccggaga cgatggacga gagcgtgaag atcgagcgcg gcacgcgcac
     4381 ccgcttcatc agcggcccgt tccacgccac gctggccatc gcgttcgtca ccgtctgcga
     4441 gaagaagttc caggcgatgt aggccgagag aaagtaggtc tcgtagtgcg gcgtctcgcg
     4501 ccggaagaga tgcgagaaaa cgaccgtgaa gatggccatg aggagcagcg gatgcaggag
     4561 cgtccagaga aagccgaaga tcgagcgctt gtagcgaacg gtgaggtcgc gatgaacgag
     4621 ctcgatcagg aggtttgcgt gtgcggccag gtcgtgaatc tcgtcccacg cgcgcagcga
     4681 ggctccagcc acattgtggc gctcctgatc ccggacgatc acctcagaac cttctacgcg
     4741 aatcgagttt ggctttcaag caatgaatgg tgagtggggc cagcggatag aatcgcagcg
     4801 gtgacgattc gaacactgct ttgctcacga ttcttagcga ctcatttcaa tcgagcgcct
     4861 tctccccccg tgcttttcgg ggggagaagg tgccgaaggc ggatgagggg ggcgttgcaa
     4921 aatgcgataa gcaggttttt cgcgatgagc ccgg
//
LOCUS       DATLVW010000183         2896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_6829, whole genome shotgun sequence.
ACCESSION   DATLVW010000183 DATLVW010000000
VERSION     DATLVW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6829"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VNN25_05870"
     CDS             complement(<1..1485)
                     /locus_tag="VNN25_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH91088.1"
                     /translation="MPSVGTNIPHDSAVGHVSGESIYIDDMAPLRGELIVDFVWSPVA
                     HGRIRGIDLDAARALPGIASLFTYRDLHHNLFGAIIQDEFLLAEDVVSFIGHPIVVIA
                     AESRAAIRAAKAAIKIDIEELEPVFTIDEAKKRGLFIGKTMTIQRGDVTSAFASAKNI
                     LEGTFVNGGQDHFYLESQAALAVPGEFDQLTVHSSTQNPSEVQDVIAHLLGLPINKVV
                     VVTKRMGGGFGGKECQATHPAAIAALVAHKLKRPARIAYGKDDDMCVTGGRHPFQNDY
                     KVAFDDDGVITAAQIEFFSDGGAFADLSTSVLGRALTHADNAYYLPNALIRGTVCRLN
                     YPPNTAFRGFGGPQGIATIETILEEIAVVLKKDPLEIRRRNCYGIDDRNTTPYGQIVR
                     NNHLPHLFDEIASRGDYHERVARVASFNASSRTHLRGIACTAVKFGISLNTKFLNQAN
                     ALVNVYLDGSVQVSTGATEMGQGVNTKIRQIVADEFGIDAERVQL"
     gene            complement(1575..2012)
                     /locus_tag="VNN25_05875"
     CDS             complement(1575..2012)
                     /locus_tag="VNN25_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXH91089.1"
                     /translation="MPADAERDLVAGIIQLANLLTRSLAPVFEKARVTPQQWAILAAL
                     EHAGQPMSLAALARMMLVSKQNMTGMIVRIEQLGLVERGDDPEDLRSSRLALTRRGRT
                     VIEKLRPAYEQWRTALAGDLNERDLATTARTIETLIENTFIHR"
     gene            2076..>2896
                     /locus_tag="VNN25_05880"
     CDS             2076..>2896
                     /locus_tag="VNN25_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91090.1"
                     /translation="MRRIADALLLGAIVIAGAVLRFGGLAVPSLWLDEILDYDVATKL
                     SHEPLWRWLTSFDSEHGPLFFATELAGRVAQTPEMAARLAPALFGVAAIVVAWIAGVE
                     CRESGVGVQGASAPTPDPRLSIPAPVFALLLAGSPLAIYYSREARPYALLMLLATAML
                     AVLLRAVPGHQGDAVVSDSRNRAKTVLIIAIPLLALFATSGATPLLIAAALTASIAFL
                     LVRRKEFVFFAGAAVLAAALVPLLYRRMPGASAAAGFRPITSRFFIRLLQSFSVSA"
BASE COUNT          464 a          909 c          942 g          581 t
ORIGIN      
        1 gagctgaacg cgctcggcat cgatcccgaa ttcgtcggct acgatctgcc ggatcttcgt
       61 gttgacgcct tgtcccatct cggtcgcgcc ggtcgagacc tgcaccgatc cgtcgagata
      121 gacattcacc agggcgttgg cctgattgag gaacttggtg ttgagggaga tcccgaactt
      181 cacggccgtg caggcgatgc cgcgcagatg cgtacgcgac gacgcgttga aactggcgac
      241 ccgcgccact cgctcgtgat aatcgccgcg cgacgcgatc tcatcgaaga ggtgcggcag
      301 gtgattgttg cgcacgatct ggccgtacgg cgtggtgttg cggtcgtcga tgccgtagca
      361 gttgcgccgc cggatctcca gcggatcctt cttcaggact accgcgatct cttcgaggat
      421 cgtctcgatg gtggcgatgc cctgcggtcc accgaagccg cgaaaggccg tgttcggcgg
      481 atagttcaag cggcagacgg tgccgcggat caacgcattc ggcaggtagt aggcgttgtc
      541 ggcatgcgtc aacgcgcggc cgagcacgga ggtcgagaga tcggcaaacg cgccgccgtc
      601 ggagaagaac tcgatctgcg cggcggtgat gacgccatcg tcatcgaacg ccactttgta
      661 atcgttctgg aacggatggc ggccgccggt gacacacatg tcgtcgtctt ttccgtaggc
      721 gatgcgcgcc ggacgcttga gcttatgcgc gacgagcgct gcgatcgcgg cagggtgcgt
      781 ggcctgacac tctttcccgc cgaatccgcc gcccatgcgt ttcgtcacca cgaccacttt
      841 gttgatcggt aggccgagga gatgcgcgat gacgtcctgc acctcggaag gattctgcgt
      901 cgatgagtga acggtgagct gatcgaattc gcccggcacc gccagcgccg cctgcgactc
      961 gagatagaaa tgatcctggc caccgttgac aaacgtcccc tcgaggatgt tcttggccga
     1021 ggcgaatgcg gaggtgacgt cgccgcgctg aatcgtcatc gtcttgccga tgaagagtcc
     1081 gcgcttcttt gcctcatcga tcgtgaagac cggctccagc tcttcgatgt cgatcttgat
     1141 cgccgccttc gccgcacgga tcgcggcacg cgactcggcg gcgatgacta caatcgggtg
     1201 accgatgaac gacaccacat cctcggctag caggaactcg tcctggatga tcgctccaaa
     1261 gaggttgtgg tgcagatcgc gataggtgaa gagactggcg atgcccggca gcgcacgcgc
     1321 cgcgtcgagg tcgatcccgc ggatgcgccc gtgcgcgacc ggcgaccaga caaagtcgac
     1381 gatgagctca ccgcggaggg gcgccatatc gtcgatataa atcgactcgc cgctgacgtg
     1441 accaacggcc gaatcgtgcg ggatgtttgt tccgaccgag ggcattgctg gaaagtataa
     1501 cggggacgtg gtgatccgca gatgtcgcag atgaaacgca aatagaggcc attctcaatc
     1561 tgcgtcgatc tgcgtcatct gtggatgaag gtgttctcga tcagcgtctc gatcgttcgt
     1621 gctgtcgtcg cgagatcgcg ctcgttcagg tcgccggcga gcgcggtgcg ccactgctcg
     1681 tatgcgggac gcagcttctc gatcacggtg cgtccgcggc gcgtgagcgc gaggcgcgac
     1741 gagcgcagat cctcgggatc gtcgccgcgc tcgaccagac cgagctgctc gatgcggacg
     1801 atcattcccg tcatgttctg tttcgagacg agcatcatcc gcgcgagcgc ggccagcgac
     1861 atcggctggc cggcatgctc cagcgcggcc aggatcgccc actgctgcgg cgtgacgcgc
     1921 gccttctcga acaccggggc aaggctccgc gtgagcaggt tcgcaagctg gatgatgccc
     1981 gcgaccaggt cccgttccgc atcggctggc atgtgtgaac gtcaatgtat tgaccaatgg
     2041 cgtgcgctgt caaatgcgcg tgctaggctt gccgcatgcg gcggatcgcc gatgctctgc
     2101 tcctcggcgc catcgtcatc gccggcgccg tgctgcgctt cggcggactc gcggtgccca
     2161 gcctctggct cgacgagatc ctcgattacg acgtcgccac gaagctctcg cacgagccgc
     2221 tctggcgctg gctgaccagt ttcgacagcg agcacgggcc gctctttttt gccacggagt
     2281 tggcagggcg cgtggcgcag actccggaga tggcggcgcg gctggcgccg gcgttgttcg
     2341 gcgtggcggc gatcgtggtg gcgtggattg cgggggtcga gtgccgggag tctggtgtcg
     2401 gagttcaagg cgcttccgct ccgactcccg acccccgact ctcgataccc gccccagtgt
     2461 tcgcgctctt actcgccggc tcgccactcg cgatttacta cagccgcgag gcgcggccgt
     2521 atgcgctgct gatgctgttg gcgacggcca tgctggctgt gctgctgcgg gcggttccgg
     2581 ggcaccaggg cgacgcggtc gtgagcgatt cgcgcaaccg cgcaaagact gttctcatca
     2641 tcgcgattcc tctgctcgcg ctcttcgcaa catcgggcgc gacgcctttg ctcatcgccg
     2701 cggcgcttac ggcatccatt gcatttctcc tggtccgccg gaaggaattt gtgttctttg
     2761 cgggcgccgc agttctcgct gcggctttag ttccgctgct ctaccggcgc atgcccggag
     2821 cgtccgctgc agccgggttc cgccccatca cctctcgctt cttcattcga ctcctgcagt
     2881 cgttctccgt gtccgc
//
LOCUS       DATLVW010000184         4705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_254891, whole genome shotgun sequence.
ACCESSION   DATLVW010000184 DATLVW010000000
VERSION     DATLVW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254891"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VNN25_05885"
     CDS             <1..411
                     /locus_tag="VNN25_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91091.1"
                     /translation="TSIPVWAAHARTLPIHFGASALASATAVLELRGHDARALNVLGL
                     AAAIAETSMGVEIESDRAITSEPLRHGATGTTIRIGGFFSGPLPLALRLLGIRSKRAR
                     RAAAASALLGSLITRLGWIEAGKASAKDPRPLLD"
     gene            484..4068
                     /locus_tag="VNN25_05890"
     CDS             484..4068
                     /locus_tag="VNN25_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH91092.1"
                     /translation="MPKLPLEVHEVLEEEYASMYGSIDRPPTGALYRDGDVIDEGWAR
                     AILQDCKIDPVNGLIAALNDLVVKGPMTKLCNSRALSDSGKSMIADYDEYTKGETDDR
                     KQEINRRIIDAAFIGAVRQLRDIRLARLYAKLHAREDDNARTALSISGGGIRSATFAL
                     GVLQGLAGAGVLDKFDYLSTVSGGGYIGSWLSSWARRHKSGISGVQSDLVRADTAVAG
                     VWPENEPKTTDGERETVVKSDLPLTKTDPEPQPVRHLREYSNYLSPQLGLTSGDTWTM
                     ASLYVRNLTLNLFVLVPLLAAALAIPRLFSLLLRHDALLEYRQLTLLTVMVAALAIGF
                     GFLGMTRPTVHGRFAGAKFFRWISTNGGFLVCIFALVVAATAMSLYWAREASKIPLQL
                     DWPWALGAIAATTVLPSVIYYLRYWRTTAVERHERMRRHSAWMKPAAEFAGAVLGVLT
                     AAALLYLLAHKVFDNPLAEVPDLTSLPPFLRGLLSSVPVSELYVCFSVPLVLLVFFMQ
                     ATVFVGISSKFNEDYDREWWGRAGAFLLMSAFFLAAGSAISVFGPVFLYRAPVILSSI
                     GGLSGLIAGLAGFSAKTPASDKEKEKGKGSKTAAASGFLSALAVPLFVVVLLAAISLG
                     TTALIQLIKGDKVPVMKAWSEQFQSTATYSQTSTTATGAKLETKYETDKTPYLSIATV
                     EGIEHLKNVHATDWPILGCIAGVALVAFGLSFLIGVNRFSMHALYRNRIIRAYLGASR
                     YHREPDAFTGFDPHDNLQMYELHPDLIWPTSFSDPAAFVVQLQNNAATAGVEREIWNA
                     LDNQTRVWIEDGHIVDAALTNCLIQDLNRILVEVKLKEPEGVDTPAARMRANRQALTD
                     TFTTSLRPRQGAPLHVINTALNLTAGEKLAWQQRKAESFTVSPLHSGSFYVGYRNSRE
                     YGGTDGISLGTAVTISGAAASPNQGYHSSPAMAFLLTLLNVRLGSWLGNPGTAGKNSY
                     DLAHPKSNLHPLALELTGMSNDQSSLVYLSDGGHFENLAIYEMVLRRCRYIVVSDGGC
                     DPKFTFEDLGNAIRKIRTDLGVPIDIKQMFMFPRTPDSKFPEGRYVATAAIRYSAIDG
                     NKTEKDGTLVYLKAGLYSETYFPKDVYNYATESADFPHETTADQFFSESQFESYRALG
                     RHVVNEICGNYDEKSENPRAPFAVQFGTISAFAKAIAEKAGKFKGATA"
     gene            4188..>4705
                     /locus_tag="VNN25_05895"
     CDS             4188..>4705
                     /locus_tag="VNN25_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HXH91093.1"
                     /translation="MPLLASLLLSTFFAINAKPQLPSVQEQSARAMLANFVASRFVAA
                     AAEFNDGLRPQITPALLAQVKDDLSAKVGTFRSVTAVHHLSQDGFRVVELIAKFEKAE
                     VSVRVVFDFENKIGAVYFNPILPPPVDAARFDVCEQITGHGLEFSIDRRRQNRVEVDR
                     TDLVLEVEHDANG"
BASE COUNT          881 a         1543 c         1379 g          902 t
ORIGIN      
        1 acatcgattc cggtgtgggc cgcgcatgcc cggacgctgc caatccattt cggcgcatct
       61 gccctggcgt ccgcgactgc agtcctcgag ctacgcggtc acgatgcgcg agcgctcaac
      121 gtactgggcc tcgccgctgc aatcgccgaa acatcgatgg gcgtggagat cgagtctgac
      181 agggcgatca catccgagcc gttgcgccac ggcgcgaccg gcaccacgat ccgcatcggc
      241 ggcttcttct ccggacccct cccgctcgca cttcgccttc tcggcatccg atccaagcgc
      301 gcccgccgcg ccgccgccgc gtccgcgctt ctcggctcgc tgatcacccg cctcgggtgg
      361 atcgaagcgg ggaaggcaag cgcaaaagat ccccgcccgc ttctggactg acggaacttt
      421 tccgcaccat tccgtctata atttgcgact acccagattc ttgcagcgtc ttgaggagga
      481 tccatgccca agcttcccct cgaagtgcac gaagtgctcg aagaagagta cgcatcgatg
      541 tacgggtcga tcgaccggcc cccaacgggt gcgctgtatc gcgacggcga tgtgatcgac
      601 gaagggtggg cgagagccat ccttcaggat tgcaagatcg atcccgtgaa tggcctcatc
      661 gcggccctca acgatctcgt cgtcaaagga ccgatgacga agctgtgcaa ctcgcgcgcg
      721 ctgtccgact ccggcaagtc gatgatcgcg gactacgacg agtacacgaa aggcgagact
      781 gacgatcgta aacaggagat caaccgccgc atcatcgacg ccgcattcat cggcgcggtt
      841 cgccaactgc gcgacattcg gctggcgcgt ctttacgcca agctccacgc tcgcgaggac
      901 gacaacgcgc ggactgcgct cagcatctcc ggcggcggca tccgaagcgc tacgtttgcg
      961 ctcggcgtat tgcaggggct tgcgggcgcc ggtgttctcg acaagttcga ctacctctcc
     1021 actgtttccg gcggtggcta catcgggagc tggctctctt cttgggcgcg ccggcataag
     1081 tcaggcatca gcggcgtgca atcggatctg gtgcgcgctg atactgcggt cgccggcgtc
     1141 tggcccgaaa acgagccaaa gacgaccgat ggtgagaggg aaacggttgt caaaagcgat
     1201 ctccctctga cgaagaccga tccggagcct cagcccgtcc gccatctccg cgagtacagc
     1261 aactacctca gcccgcaact cggactgacc tcgggcgaca catggacgat ggcgtcgctg
     1321 tacgtccgga atctgacgct gaatctcttc gtgctcgtgc ccttgcttgc cgccgcgctg
     1381 gcgattccgc ggctcttctc gctgctactg cgccacgatg cgttgcttga ataccggcag
     1441 ctgactctgc tcaccgtcat ggtcgctgca ctcgcgatcg gatttgggtt cctcggcatg
     1501 acgcgtccaa cggtgcacgg ccgcttcgcc ggcgcgaagt tctttcgctg gatctcgacc
     1561 aacggcggct tcctcgtctg catcttcgca cttgttgtcg cagccaccgc gatgagcctt
     1621 tattgggcgc gggaagcgag caagataccg ctccaactgg actggccgtg ggcgttgggg
     1681 gcgatcgctg ccacgaccgt gctgccgagc gtcatctatt acctccgcta ctggcgcacc
     1741 accgccgtcg agcgccatga acgcatgcga cgacacagcg cctggatgaa gcccgctgcg
     1801 gaattcgctg gtgcagtcct cggcgtcctg acagcggccg cgctgctcta cctgctcgct
     1861 cataaagtgt tcgacaatcc gctggccgag gtgccggacc tgacgtcgct gccgccgttc
     1921 ctccgaggac tgctatcgag cgtgccggtt tcggagttgt acgtttgctt cagcgtgccg
     1981 ctggtcctgc tcgtcttctt catgcaggcc acggtcttcg tcggcatctc cagcaagttc
     2041 aacgaagact acgaccgcga gtggtggggc cgcgcaggag cgttcctcct gatgagcgca
     2101 ttcttcctcg ccgccggcag cgccatctcg gtcttcgggc ccgtgttcct ctatcgcgcg
     2161 ccggtgatcc tcagctcaat cggcggactt tcgggtctga tcgccgggct ggccggcttc
     2221 agcgcgaaaa cgcccgccag tgacaaagaa aaagaaaagg gaaaaggcag caagaccgcg
     2281 gctgcgagcg gcttcctctc cgcgcttgcg gtgccgctgt tcgtcgtcgt gcttctcgcc
     2341 gcgatttcgc tcggtaccac ggccctaatc cagctcatta agggcgacaa ggttccggtg
     2401 atgaaggctt ggtcggagca gttccagtcc accgcaacct attcgcagac ttccaccacg
     2461 gctactggcg cgaaactcga gacgaagtac gagaccgata aaacgccgta cctctcgatc
     2521 gcaaccgtcg aaggcatcga gcatctcaag aacgttcacg ccaccgactg gccgatcctc
     2581 ggatgcatcg ccggcgttgc gctcgtcgcc tttgggctct cgtttctgat cggcgtgaac
     2641 cgtttctcga tgcacgcgtt gtatcgaaac cgaatcattc gcgcctacct cggcgcatcg
     2701 cgctaccacc gcgagccgga cgcgttcacc ggcttcgatc cgcacgacaa tctgcagatg
     2761 tacgagctcc atccggatct gatctggcca accagcttca gcgatccggc ggcattcgtg
     2821 gtccagcttc agaacaacgc cgcgaccgcc ggcgtcgagc gggaaatctg gaacgcgctc
     2881 gacaaccaga cacgagtctg gatcgaggac ggacacatcg tcgatgccgc tctgaccaat
     2941 tgtctgattc aggacctcaa ccgcatcctc gtcgaggtaa agctcaagga gccggaaggg
     3001 gtcgacacgc ccgcggcgag aatgcgggcg aaccgccagg ctctgaccga caccttcacg
     3061 acttcgctgc gtccgcggca gggtgcgccg ctgcacgtca tcaataccgc gctcaacctc
     3121 acggccggcg aaaagctggc gtggcagcaa cggaaggccg agtccttcac cgtctcgccg
     3181 ctgcactccg gcagcttcta cgtcggctat cgcaactcgc gagagtacgg cggaacggat
     3241 ggcatttcgc tcggcacggc ggtcaccatc tccggggcgg ctgccagtcc gaatcagggt
     3301 taccactcct caccggcgat ggcgttcctc ctcacgcttc tcaacgtccg gctcgggtcg
     3361 tggctgggca accccggcac tgctggaaag aattcgtacg acctcgcgca tccgaaatca
     3421 aaccttcatc cgctcgccct cgagctcacc ggcatgtcga acgaccagtc gtcgctcgtc
     3481 tacctttccg acggcggaca cttcgaaaac ctcgccatct acgaaatggt tctgcgccgc
     3541 tgccgctaca tcgtggtcag tgacggcgga tgcgatccga agttcacctt cgaagacctt
     3601 ggcaatgcca tccgcaagat ccgcacggac ctcggcgtgc cgatcgacat caaacagatg
     3661 ttcatgtttc cgcgcacgcc cgacagcaag ttccccgaag gccgatacgt ggcgacggca
     3721 gcgattcgct acagcgctat cgacggcaac aagaccgaga aagacggcac actcgtctac
     3781 ctcaaggccg gactttattc ggagacctac tttccgaaag acgtctacaa ctacgcgacc
     3841 gagtcggcgg actttccgca cgagacgacg gccgatcaat tcttcagcga gtcacaattc
     3901 gagagctacc gcgccctcgg gcgtcacgtc gtcaacgaga tctgcggcaa ctacgacgag
     3961 aagtcggaga acccgcgcgc tccgtttgcc gttcagttcg gaaccatctc ggcattcgcg
     4021 aaagcgatcg cggagaaggc cggcaagttc aaaggcgcga cggcgtgaag taagaatgct
     4081 cgacgaacct cgagtttttg gaggttagtc tctgcggtcg aatggcgctg gtaccatagc
     4141 ggctgccgtc gccgcaccaa cgccatccga ccggagaaat actgcccgtg cctcttctcg
     4201 cctcgctact gttgtccacg ttcttcgcca tcaacgccaa gccgcagttg ccgtcggtac
     4261 aggagcagtc ggcgcgggcg atgctcgcaa acttcgttgc cagccgcttc gtagctgctg
     4321 cggcggagtt caatgacggg ctgcgtccgc agatcacgcc ggctctgctg gcgcaggtga
     4381 aggatgatct gagcgccaaa gtggggacgt ttcggtcggt gaccgcggtc catcatctca
     4441 gccaggatgg ctttcgcgtg gtcgaactga ttgcgaagtt cgagaaggcg gaggtctccg
     4501 ttcgcgtcgt gttcgacttc gagaacaaga tcggtgcggt ctatttcaat ccgattctgc
     4561 cgccgccagt cgacgcggcc cgcttcgatg tttgcgagca gatcacgggc catggtctcg
     4621 agttcagcat cgaccggcgg cggcagaatc gggttgaagt agaccgcacc gatcttgttc
     4681 tcgaagtcga acacgacgcg aacgg
//
LOCUS       DATLVW010000185        18450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_52316, whole genome shotgun sequence.
ACCESSION   DATLVW010000185 DATLVW010000000
VERSION     DATLVW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52316"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..3847
                     /locus_tag="VNN25_05900"
     CDS             32..3847
                     /locus_tag="VNN25_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH91094.1"
                     /translation="MPLDKKYLTLQFWLKVHQKSGIEFQTFFEQIMEKAYPDFQKIRP
                     YGQEGDKGNDGYRPSEGVYYQAYAPSHPSEKEAAAAEKFKDDFGKLKKGWDKISTIRE
                     LNFVYNEKGSGLTVKLEEAKAELNAANPQIEFKVFTPKDLEPIFLSLSSEHIASLGFD
                     IDSRNAVQSARDQLTRLDEELDKESGEFVLRVLRTINEIIVGQDDESLSLDYEILESR
                     ALQKAEKVREAREKLESIVRRYPNDARAFSYLADIHINLDDFERNADLLREAERIAPD
                     SGLLRVQKLIREIRLGTTFDPATIDENSFPSDPRTKANVYRLYGSLLTRTDDYKQAAA
                     YVERALHLNPDKFHSHDSKIALMEEELHAEADYKKRHDLGLGVLKAIDEVETRFNEGG
                     GLGARGQSLLNVRRIHAYLATENYAALERTAKDTLELVLGCYFDRLIDGITADTIHWV
                     GLSPSDFTRLRAYFKAADKPISDSLAKILVLQFLYRETLFTEGKAFFDEIGRKSIVEF
                     ISAIEGADFDHVLAFVGKDVQFAVDLALGLREPAALKQKLIESVPDDGSVNKDKLSLI
                     VYYEAGELDKAFALVQKLNLAALSYIECLNFLRVAYKMKAWDSAVVLLEKILAHEQDA
                     QSALQAKLQLFNANFHLERFAEVIRIGRGIVEDPSGLALLDDYNKETLVAHTALAYLK
                     RGDAGAKEFVQGHEQFMRSFEAKIGVQGEVYVKSGEPGAALRSVVEGVRILRHPTPEQ
                     YGRLFLIFGEIGNLMPEFTLESTARVGQGLFVKLRELERWFYIGAGDELDATKVAEGD
                     PYYAALLGKELGDQVALPNRYRAASPEHVIEAILPVEKYILWQSRHHALKLSAEQRWD
                     AIELIEVPTTDGSIDLQYLIARMEDDDKKRREFFTLYCEQDVPLALLAINDGGLAGAI
                     GHIASAQRGFIKATSGSLDEFNQQKDVAKVIIAGEPIYLDGTSALMLSETGLFARVYE
                     FIPGLKVPQSVVSLLFELRDKFAYHPGQSGHIAYVDGKIIFSELNTEKNDAIRANFAT
                     TVAILEGKPDNIVAISAANKASGFMEQRVPASLADACILAQRDGVAVLTDDFFYVKAN
                     EIETKKPAPAYCSSLALLRVLYEEGKVGFEEYLDYFDHLASYRVRFLPLTTDDLEKAV
                     FGDQTIKVVKPEQLRKFQFGLTLSEEYGVPQRAAHDVVGSFLFRVLVDDSLSPEIATR
                     IFVEIVTTFPTTKSRKMFGQMLMFLTVQAINVSRRSIVMGSTKAVQAKVDALAEFLST
                     YGRDELIVT"
     gene            complement(4179..5735)
                     /locus_tag="VNN25_05905"
     CDS             complement(4179..5735)
                     /locus_tag="VNN25_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXH91095.1"
                     /translation="MKQLFAYIRVSDPKQGKGVSLDEQHSIIEAYAIRIGAVIVEWFV
                     EKKTAAKAGRPQFTRMVRLLRQGKAHGVVIHKVDRSTRNFRDWADIDELIESGIDVHF
                     ANEHMDLRSDGGRLAADIQMVFAVQYIRNLRQETLKGIHGRLKQGILPRNAPIGYLDC
                     GAGKPKTIDPVRGPLIRALFERYATGALTLRMLTEDATGAGLRNRNAKPLRLTQIHAM
                     LRNPFYVGILRSSRFGVFPGAHEPLVTKAMFDRVQAVLAGKFIRRTKRHILLFRRILH
                     CKTCGRSLIGSERKGNVYYRCSNVECPTTSVREDAVEEAVSAILASLRLDKHDALTIA
                     AELAEYDRDRTALQEARRLSLTETLSAANARLTRLTDLLLDGKIDAATHAAKHAELVL
                     ERRDLERDLAAIENGTQELVRRTRQIVELAHDAANIYRIADHERKRRLLETLLSNCVV
                     TGKTLEFSLLEPFATFARRHSEQTCSPDWYTPRTFSLDELLKWSAELPDKLGDVLADS
                     ELTDEVETVA"
     gene            complement(5732..5959)
                     /locus_tag="VNN25_05910"
     CDS             complement(5732..5959)
                     /locus_tag="VNN25_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91096.1"
                     /translation="MLDTTKNALRALLPDANDTDIQAAEERFHRYLELAIEVYQVAAQ
                     NTVSELLTQSDGRGTVIAGQVEPPRTLTNTG"
     gene            5946..7055
                     /locus_tag="VNN25_05915"
     CDS             5946..7055
                     /locus_tag="VNN25_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HXH91097.1"
                     /translation="MVSSMRITLLSRHVLARVRKYPAAGQGACGGTSAPSSATIPPVT
                     LSGMSNIINWPRTRAQKFHNFLPEEIGSYLKGRGIPATIIESHLLGWNGERITFPIFG
                     ENPRKVLGFRYAKVATDISDTPEMISDPDLGVQLYGLDKVLQKPSRVVICDGEFNRLV
                     LEANDFAAVCSTGGADAFLKEWALYFEGVKQIFICFNRDRTGEAAAKKLQSILPRARI
                     VKLPACTLDLTDFFVFQEKTKLDFEILLAAASMEEKDPADTPPSVREFRPLLKSVQRR
                     AEHLRKNVKLHDIVEQFATLQARGKRLVAHCPFHDDRELSFNVYPAANTYACSRCGAK
                     GDVVQFLMDKESMTFSEALDALESFEFTHEIYGTG"
     gene            7042..8421
                     /locus_tag="VNN25_05920"
     CDS             7042..8421
                     /locus_tag="VNN25_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91098.1"
                     /translation="MAPAEPEKKPQPEQKPTITPTASAVLPNGELVEMVYDPVERKTQ
                     FVTGTAEAWGYEESVLLSPLERLVPYSPTNNLVKHGVVLFASEPTEYGDEATLVSRIR
                     DFVHRYVDISEDFETIATYYILFTWVYDAFHEVPYVRIRGGYGTGKTRFLLAVGSLCR
                     FAIFASAASTVSPLFRLLDAFRGTLILDEGDFRASDEKSEVVKILNNGNARGFPVLRT
                     EVSPKKEFDPRAYHVFGPKIVATRGFFQDRALESRCVTEDMRGGSLRREIPLNLDDSF
                     HEEARNLRNQLLLYRLRHFKDARGQTRAGARDLEPRLQQIFGPLVSVIEDASARDRVV
                     AVLGKLNDDAVEERSETAEAQVLSVIRQLVAKGPDSRLAVQDITSAFLARYGHEYERR
                     ITPHWIGSIIRRRLGLRASKSTGTYLLRPEDVKRLPALYVRYGLTSAEKNDPGSPAVE
                     EGETNHGTS"
     gene            8439..9605
                     /locus_tag="VNN25_05925"
     CDS             8439..9605
                     /locus_tag="VNN25_05925"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HXH91099.1"
                     /translation="MIGETYWRDMRKRFGSRPADRRAHMYVIGKTGTGKTSLLEGMIR
                     QDILAGHGLALFDPHGDLAERLALWMPESRKADLIYLDVPDPTQQFGFNPLEGVSPLR
                     RSVAANGIVEALKKIFSEAWGVRLEYILRSAVVLLLEQPEATLADIVRLFHDKDFRKA
                     AAERSENEQVKRFWTTEFEKLGRQRSEAVAPIENKLGSLLVDPFVSRILTASKSTFNP
                     RAAMDEGKVLLVNLSKGKIGEAPAMLFGGLLVTALGLAGLGRADVPQKERRDFFIYMD
                     EFQTFTTLALANMLAELRKYGVGLVLANQYMDQIEPEIRTTILGNAGTLVVFRVGAVD
                     AGKLVKELRPDLEPYDLTLLSNRTFWIRPLVNGESVEAFTGKTITLEESKNSGL"
     gene            10160..10663
                     /locus_tag="VNN25_05930"
     CDS             10160..10663
                     /locus_tag="VNN25_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91100.1"
                     /translation="MKRLIDPRAILGIDPTKRGLAFVFFENGRLIDWGIRHTGNEIAN
                     LEAILKLCPADVVVLEDGSAERCERRPQMRKVLSRLTRAAKQRGLEIVCVSRFAVRCA
                     WRARGVTRKHAVAKAIAEEFPALEVFVPRPRIKAYMDEEARVHVFDAASLVLHTFGVA
                     DGDSLAA"
     gene            10790..11008
                     /locus_tag="VNN25_05935"
     CDS             10790..11008
                     /locus_tag="VNN25_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91101.1"
                     /translation="MNPIITLSCPDCSRTVETFRLSASPTTVTLQATCHPCKTIAEVT
                     LTFEDLRDLGAGRCVAANFIASTPELVM"
     gene            11816..12031
                     /locus_tag="VNN25_05940"
     CDS             11816..12031
                     /locus_tag="VNN25_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91102.1"
                     /translation="MSDLIAFKSGKDDGEETRIALVETVTCVECGKVETDEQAYDEGW
                     QIDPVVCPDCLHWTLTPEGECCLGGAS"
     gene            12356..12679
                     /locus_tag="VNN25_05945"
     CDS             12356..12679
                     /locus_tag="VNN25_05945"
                     /inference="COORDINATES: protein motif:HMM:NF044570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91103.1"
                     /translation="MLFRDSSGSFSYTRGVNGVCQIHGLYWFRDFIAAQQKRVKRDPR
                     FRKVQRWVHKVQFRRATIYCYAGDSDETPLIFPQHLYCDVTEPEITFYLEGDVLRTKE
                     ERDAV"
     gene            12669..13007
                     /locus_tag="VNN25_05950"
     CDS             12669..13007
                     /locus_tag="VNN25_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91104.1"
                     /translation="MRCEKCGVAYSAIIHITRVPFVGWLCIPCAEEIREKGGNAMLRY
                     ERYKRRFWAVYEDDGLLCVTVYLKGALSVIERITGVRPERPVRKKRAPLPPIYRPKPT
                     QERQWIIHDR"
     gene            13028..13396
                     /gene="ssb"
                     /locus_tag="VNN25_05955"
     CDS             13028..13396
                     /gene="ssb"
                     /locus_tag="VNN25_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXH91105.1"
                     /translation="MNNLNRVILTGYLGKDPETAPAASTTVTKFSLAVTDRWKDAAGE
                     RQERTNWLNVVVWNGNGENVAQYLKKGSHVLIEGSLRTSEWDDKDTGQKRWKTEIHAS
                     QVIFLDKKAEEPRKRRKKAV"
     gene            13396..13668
                     /locus_tag="VNN25_05960"
     CDS             13396..13668
                     /locus_tag="VNN25_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91106.1"
                     /translation="MFAVVSGETLQCPHCLSTDSDHFAVERRVLWREPILEMFEDKGL
                     LVLIADCDNALVDMAGSGPDCLYCANCGNRSDIDVTTLVHHSDPKV"
     gene            14117..14461
                     /locus_tag="VNN25_05965"
     CDS             14117..14461
                     /locus_tag="VNN25_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91107.1"
                     /translation="MDYAQVLLHAEFEHGKPLGDTAAVVSLPPKVIDDHEIVYRVRRA
                     PDMKRGDLLVIEPRSTAATGELVVATRGKNAYVGHWWAKHGRKELLEHPNKHLTGEFQ
                     IIGAVNLIVRLS"
     gene            14458..14673
                     /locus_tag="VNN25_05970"
     CDS             14458..14673
                     /locus_tag="VNN25_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91108.1"
                     /translation="MTKLEAFIKSRKLKPIRIAFEADYSRQHLLRIRKGQMEPTRKCI
                     KAITVAIRRLTREPITASDLFDLDEET"
     gene            14677..15057
                     /locus_tag="VNN25_05975"
     CDS             14677..15057
                     /locus_tag="VNN25_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91109.1"
                     /translation="MRKTLRLRLITATQFEQFFLLDSPGYFIARDAMNAGETATATDA
                     LEVLCLANQLYESELLNRLIVELATLGKLDEYIEKRGTDEGGEFIVEVFGKEKALRIL
                     QDVTNEVKRQSKVGTGENDKAVSR"
     gene            complement(15266..15793)
                     /locus_tag="VNN25_05980"
     CDS             complement(15266..15793)
                     /locus_tag="VNN25_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91110.1"
                     /translation="MVRKACMANPGKATPTPPQDLSQSFQRLAESANRLNKASDELSR
                     AIGPIEAVFKKLNLGIAKWHVFFGPFPDQDGDYHQLEIGYAKMGGKWCIALAEESGNV
                     HASDPDYGSRTSWAFNDAPRQLRIRAVKEIPKLLEALVEEADKVTSELEVETRRAQEV
                     GETLTTLAATSGVRR"
     gene            complement(16001..16906)
                     /locus_tag="VNN25_05985"
     CDS             complement(16001..16906)
                     /locus_tag="VNN25_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXH91111.1"
                     /translation="MSVEDAVTKYLRDAETRLKASTVDLYRRGLKHLLSWCEAEGVKA
                     LPELDLEKLRTYREAWTCKPVTAARRIDRLKIFFGFCLDTGWIQKNPAKALKPPEVRT
                     VGKEPFSEEELPKIFEACGRLITRGTYGKENVKRVRAFIYILRYTGLRISDAASLRVS
                     DVRDGKVLLRTEKTGTLVWIPIPQFVVDALAAVPRVSEYYFQTGKAKPKTVRGGWDRT
                     IRTVLTLAEVNHGSAHSFRTTLAVDLLNKGIAVETVAAILGNSPAIVIKHYAPFVKSR
                     QVALETAVRSLWVEPKPQLALVKTA"
     gene            complement(17203..17280)
                     /locus_tag="VNN25_05990"
     tRNA            complement(17203..17280)
                     /locus_tag="VNN25_05990"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17243..17245),aa:Pro,seq:cgg)
     gene            complement(17402..18052)
                     /locus_tag="VNN25_05995"
     CDS             complement(17402..18052)
                     /locus_tag="VNN25_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HXH91112.1"
                     /translation="MFTAMKGRLLVILIALFAAPLLAQHRVDVLVDAEGARRPGRNAD
                     FSTGVHFDPEFGTGGGAGLGLNFFLTDRVSIEAKAAALESQLKVRTIGSDFFAVADLG
                     RAQIYPITALLQWHLTEHGTIRPYLGAGVAHVILRNINHSVGSTGASGIHFKDPTGIV
                     VDGGLEVQLSKRWSILGDARYIPIETKSEAAFIGTSSSVKMNVRPLIVSTGLSWRF"
     gene            18134..>18450
                     /locus_tag="VNN25_06000"
     CDS             18134..>18450
                     /locus_tag="VNN25_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91113.1"
                     /translation="MSMRFFPVLILAAAPLFAQQPPPPPQPSPTFGEKVDVNLVQLDA
                     VVTDNRGNQMLGLTKDDFIVRENGVQQTIDTVDYFTNRRLIDAPEALAKFKVERVHDE
                     RYL"
BASE COUNT         3947 a         5396 c         5316 g         3791 t
ORIGIN      
        1 ccaaagtcaa ccagctcgca aaacgctcat gatgccactc gacaagaagt atctaacgct
       61 ccagttctgg ttgaaggttc accaaaaatc tggaattgag tttcagacct tcttcgagca
      121 aatcatggag aaggcgtacc ccgattttca gaagatccga ccgtacggcc aagagggcga
      181 taagggcaac gatggttatc ggccttccga gggcgtctac taccaggcat atgccccctc
      241 gcacccaagc gagaaggaag ctgccgctgc cgaaaagttc aaggacgact tcggaaagtt
      301 gaagaagggc tgggacaaga tctccacaat ccgggagctg aatttcgtat acaacgaaaa
      361 gggatccggc ctcacggtga aacttgagga agcgaaggcg gagctgaatg ccgcaaaccc
      421 gcagattgaa ttcaaggttt tcacgccgaa ggacctggag ccgatctttc tttcattaag
      481 tagcgagcac atcgcgtctc tgggatttga tatcgactcg cgaaacgcag tgcagagcgc
      541 gcgtgatcag ctgacgagac ttgacgagga actggataag gaaagcggcg aatttgtgct
      601 gcgagttttg cgcactatca acgagatcat cgtcggacag gacgatgaaa gcctttcgct
      661 tgattacgaa atcctggagt cgcgagcact gcagaaggcc gagaaggtac gggaagcccg
      721 cgagaagcta gagagtatcg tacgacgata tccgaatgat gcccgggcgt tctcgtacct
      781 agccgacata catatcaacc tcgacgactt tgagcgaaac gctgaccttc ttcgagaggc
      841 tgaacgaatc gctcccgatt ccgggttgct acgtgtccag aaactgattc gcgagatacg
      901 gctcggaact acgtttgatc ctgcgacgat tgacgagaac agctttccgt ctgacccccg
      961 gaccaaggca aacgtctacc gtttgtacgg ctcacttctg acgcgcacgg acgactacaa
     1021 gcaggccgca gcgtacgtag agcgagctct acacctgaat ccagacaagt tccacagcca
     1081 tgacagcaag atcgctctaa tggaggagga gcttcacgca gaagccgact acaagaaacg
     1141 acatgatttg ggtttgggtg tgctgaaagc aatcgacgag gtcgaaacta gattcaatga
     1201 aggcggtggc ctgggcgcgc ggggccagag cctgctgaat gtccgccgga ttcacgccta
     1261 cctggctaca gagaattacg ccgcgcttga acggaccgcc aaagacacgc ttgagctggt
     1321 gctcggctgc tattttgatc ggctcatcga tggtatcacc gctgacacga tccattgggt
     1381 cgggctgtca ccgagcgact tcactcgcct gcgagcttat ttcaaagcgg cggacaaacc
     1441 gatatcggat tccttggcaa agatactagt cttgcagttt ctttacaggg aaacgctctt
     1501 cacggaaggg aaagccttct tcgatgagat tggcaggaag agcattgttg agttcatcag
     1561 cgcgatcgaa ggcgcagact tcgatcacgt cttggccttc gttggaaagg acgtgcagtt
     1621 cgccgtcgac ctcgccttgg ggctgcgaga accggctgcg ctgaagcaga agctgatcga
     1681 atcggtacct gacgatggga gtgtcaacaa agataagtta tctcttatcg tctactacga
     1741 agctggggag ttggacaagg catttgccct cgtccagaaa ctcaatcttg cggcgctaag
     1801 ctatatcgag tgcctaaact tccttcgcgt tgcctataag atgaaggcat gggattccgc
     1861 cgttgtttta cttgaaaaga tattggctca cgagcaagac gcgcaatccg cactccaagc
     1921 caaactgcag ttgttcaacg cgaactttca tctggagcgt ttcgcggagg tcattcgtat
     1981 cggtagaggc attgtagaag atccttctgg cttggcattg ctcgacgact ataacaagga
     2041 gaccttagtg gcgcatacgg cgctagcgta cctgaagcgg ggcgacgctg gcgcgaaaga
     2101 gtttgtccaa gggcatgagc aattcatgcg ctcttttgag gcgaagattg gcgttcaagg
     2161 ggaagtctat gtgaagagtg gcgaaccggg agcggctctt cggtcagtcg tggagggcgt
     2221 caggattctg cgacatccga ctccggagca atacgggagg ctgtttctca tctttggcga
     2281 gataggtaac ttgatgccgg agtttacgct cgaatccact gcgcgtgtcg ggcagggcct
     2341 attcgtgaag ctgcgcgagc tagagcgatg gttctacatc ggcgcagggg atgagctcga
     2401 cgctacgaag gtggccgaag gcgatccata ttacgcggca ttgctcggaa aggaacttgg
     2461 agaccaagtc gcgttgccaa atcgctatcg agcagcgagt cccgagcacg tcatcgaagc
     2521 gattctgccg gtggaaaaat acatcctgtg gcaatcgcgg caccacgctc tgaaattgtc
     2581 ggccgagcaa cgatgggatg cgattgaact catagaggtc ccgactaccg acggctcgat
     2641 tgacctgcag tatttgatcg ccagaatgga ggacgacgac aagaagcgaa gagagttttt
     2701 cacgctgtac tgcgaacaag atgtaccact cgctctcctc gccatcaacg acggtggact
     2761 ggcaggcgcg attggccata ttgccagtgc ccagagaggt tttattaaag cgacgagcgg
     2821 tagcctcgac gagtttaatc aacagaaaga tgtcgccaag gtcataatag cgggtgaacc
     2881 aatctattta gatggcacgt cggcgttgat gctttctgag acggggcttt tcgcgagggt
     2941 ctacgagttc attcctggtc tgaaagttcc acagtcggta gtgtcactgc ttttcgagct
     3001 tcgggacaag tttgcgtatc atccaggcca gtccggccat atcgcttatg tggacgggaa
     3061 gataatcttc tcggaactca acacagagaa aaacgacgcg atcagggcaa acttcgcgac
     3121 gacggtggcg atactcgaag ggaaaccgga caacatcgtc gcaatctctg cggcgaacaa
     3181 agcgagcggc ttcatggagc agagagttcc tgcgagcttg gccgacgcat gcattctagc
     3241 gcagagggac ggcgtcgcgg ttctgacgga tgacttcttt tacgtgaagg cgaatgagat
     3301 cgagacgaag aagccggcgc cggcgtactg ctcgtcgttg gcattactcc gagtcttgta
     3361 tgaggaaggc aaagtcggct tcgaggaata cctagattac ttcgaccacc tggcgtcgta
     3421 tcgagtacgg tttcttcctt tgacgacgga cgatctggag aaagcggtat tcggtgatca
     3481 aacgatcaag gtggtgaagc cggagcagct ccgtaagttc cagttcggtc tgacgctttc
     3541 cgaggaatac ggcgtgccac aacgcgcggc gcacgacgtt gtcggcagct tcttgtttag
     3601 agtattggtg gacgactctc tgtcacccga gattgctacg cggattttcg tagagatcgt
     3661 gaccaccttt ccgaccacca agagtcggaa gatgttcggt cagatgctga tgtttctcac
     3721 ggtccaagcg atcaatgtga gccgacgatc aatcgtaatg ggttcgacaa aggcggtgca
     3781 agcgaaggtt gatgcgctcg ctgaattcct tagcacgtac ggccgcgacg agctgatcgt
     3841 aacgtaatcg gctcaaaagg ccgcgacgac tgcaagacgc tgcgacctgg gccatgtcta
     3901 cttcaccgcg gcctgaagca gctggcgcgc tttacctagg gtgcgtagct cttctcgatg
     3961 atcctcaccg aggaatggcc gagaaggatc gacaatctac tcactggtcg cataccaggt
     4021 gaacgcgagc cgcacgagtg gtcctcgtta cggattgatc tccttcgcgt cgctcacgcc
     4081 gcagtccagt accagcaata ccggacttca gggactttgg ggacttgaaa cggaaggacc
     4141 cgccccgtgg cgggtccttg tatccgtgtc tctggaacct aggcgactgt ctcgacttca
     4201 tccgtcaatt cgctatcagc gagaacatcc ccgagtttat ctggcagttc ggcgctccac
     4261 ttgaggagtt cgtccagcga aaaagtccga ggcgtgtacc aatcggggct acacgtctgt
     4321 tccgaatgcc gtctcgcgaa cgtcgcaaac ggctcaagca aggaaaactc gagcgttttg
     4381 ccagtgacta cgcagttcga caatagcgtt tcaagtaaac ggcgttttcg ctcgtgatcg
     4441 gcgattcggt aaatatttgc cgcgtcgtgc gcgagttcga caatttggcg tgttcggcgg
     4501 accagttcct gggttccgtt ctcaatagca gcaaggtctc gttctaagtc tcggcgttcg
     4561 agtaccagtt cggcgtgctt tgcggcatgc gtagcagcgt cgattttacc gtcgagaaga
     4621 agatcagtga gccgcgttag gcgcgcgttc gcagccgaaa gggtttcggt gagcgagagg
     4681 cgacgagctt cctgaagcgc tgtacgatcc cgatcgtatt cggcgagttc ggcagcaatc
     4741 gtgagcgcgt catgcttgtc tagccgtagt gacgcgagga tcgcgctgac tgcctcctcg
     4801 acagcgtctt cgcggactga ggtcgtggga cactcgacgt tggagcagcg gtagtacacg
     4861 ttgcctttgc gctcggaccc tattagagac cggccgcagg tcttgcagtg gagtatccgc
     4921 cggaagagaa ggatgtgccg cttagttcga cggatgaact tgccagcgag aacggcctgc
     4981 acccgatcga acatcgcctt ggtgacgagc ggctcgtgcg ctcctgggaa cacgccaaag
     5041 cgtgacgaac ggaggattcc cacgtagaaa gggttgcgga gcatggcgtg aatctgcgta
     5101 aggcgcagcg gtttggcgtt gcggttccgg agcccagccc ccgtcgcgtc ttcggtgagc
     5161 atgcgtaggg ttaaagcgcc ggtcgcgtac cgttcgaaga gggctcgtat gagcggcccc
     5221 cggacgggat cgatggtctt tggtttgccg gcgccacaat ccaagtagcc gatcggcgca
     5281 ttacgcggca ggattccttg cttcaggcgc ccatggattc ccttgagcgt ttcctggcgg
     5341 aggttcctga tgtactgcac agcaaagacc atctggatat cggccgcgag gcgtccgccg
     5401 tctgagcgga gatccatgtg ctcgtttgcg aagtggacgt cgatgccgct ctcgatcaac
     5461 tcgtcgatgt ccgcccagtc gcggaagttc cgggtactgc ggtccacctt gtggatcacg
     5521 accccgtggg ccttgccttg gcggagcagt ctcaccatgc gggtgaactg cggccggccc
     5581 gctttggcgg cggttttctt ttcgacaaac cactcaacga tgacggcgcc aatccggata
     5641 gcatatgcct cgattatcga gtgctgctcg tcgagcgaca cgcctttgcc ttgcttcgga
     5701 tcagagacac ggatgtaagc aaagagttgc ttcatccggt gtttgttaag gttcttgggg
     5761 gctcgacctg ccccgcgatt acggtacctc gtccatccga ttgcgtcaaa agctcactta
     5821 ctgtgttctg cgcggctacc tggtaaacct cgattgcgag ttccaagtat cgatgaaacc
     5881 gttcctccgc cgcctgaatg tcagtgtcat tagcatcagg aagcagcgcg cgcagcgcgt
     5941 tcttggtggt gtcgagcatg agaatcactc tactgagccg acacgttttg gcgagggtgc
     6001 gtaagtaccc tgctgcggga cagggagcgt gcggcggtac ttctgccccc tcgtcggcga
     6061 cgattcctcc cgttacgctg agcggcatgt caaacatcat taattggccg cgtacacgcg
     6121 cgcagaagtt tcacaacttc ctgccggagg agatcgggag ctacctcaaa ggtcgaggca
     6181 ttccggcgac tatcatcgaa agccatctac ttggctggaa cggcgaacgg atcaccttcc
     6241 caatcttcgg tgagaacccc cgcaaggtgc tcggtttccg ttacgccaaa gtggcgaccg
     6301 atatctccga cacgccggag atgatttccg accccgatct cggcgtgcag ctttacgggt
     6361 tagataaggt gcttcagaaa ccgtctcgcg tggtgatttg cgacggtgag ttcaatcgcc
     6421 tcgttcttga agcgaacgat ttcgctgcag tctgctcgac gggaggcgcg gacgctttcc
     6481 ttaaggagtg ggcgttgtac tttgaggggg tgaagcaaat cttcatctgc ttcaaccgtg
     6541 atcgcactgg tgaagcggcc gcgaagaaac tccaatccat cctgccgcgc gcgcggattg
     6601 tgaagctgcc ggcctgcacg ctcgacctga ccgacttctt cgtgttccag gagaagacga
     6661 agctcgattt cgagatcctg ttggccgcgg cttcgatgga ggagaaggac cccgccgaca
     6721 cgccgcccag cgtgcgcgaa ttccggccgc tcctcaaatc cgtccagcgc cgcgctgaac
     6781 atctgaggaa aaacgtgaag ctccacgaca tcgtcgagca gttcgcgacg ctccaggcgc
     6841 gcggcaagcg gctcgtggcg cactgtcctt tccatgacga ccgggagctt tccttcaatg
     6901 tgtatcccgc ggcgaatacg tatgcctgtt cccgatgcgg cgcgaagggt gacgtcgtgc
     6961 agttcctgat ggataaagag tcgatgacgt tctctgaggc cctcgacgcc ctagagagct
     7021 tcgaattcac tcacgaaatc tatggcaccg gctgaacctg agaaaaaacc gcagccggag
     7081 cagaagccaa ccatcacgcc aaccgcctcc gccgttctcc cgaacggcga gctggttgag
     7141 atggtctatg acccggtcga gcggaagacg cagttcgtca cgggaacggc ggaggcatgg
     7201 ggctatgagg aaagtgtcct tctttctcct ctcgaacgcc tcgtgccgta ctcaccaacc
     7261 aacaacctcg taaagcacgg cgtggtgctt ttcgcatccg aaccgaccga gtacggcgat
     7321 gaggcgacgc tcgtctcccg tatccgggac ttcgtgcatc gctacgtgga tatctcagag
     7381 gattttgaga cgattgctac ctactacatc cttttcacct gggtctatga cgccttccac
     7441 gaagtcccct atgtccgtat ccgcggtggc tacggtaccg gcaagacgcg ttttctgctg
     7501 gcggtaggtt cgctctgccg cttcgcgatc ttcgcgtcgg ccgcgagtac cgtctcaccg
     7561 ctcttccggc tcctcgacgc cttccgcggg acgctcatct tggacgaggg agacttccgg
     7621 gcctccgacg aaaaaagcga agtcgtgaag atcctcaata acgggaacgc gaggggcttt
     7681 cccgtcctcc gcacggaagt ctcgccgaag aaagagttcg acccgcgggc gtatcacgtc
     7741 ttcgggccga agatcgtggc tacgcgcggc ttcttccagg accgggcctt ggagagccgc
     7801 tgcgttaccg aggacatgcg cggcggctcg ctccgacgcg agattccgct gaatcttgac
     7861 gactcgttcc acgaggaggc ccgaaacctg cgcaatcagt tgctgctcta tcgtctgaga
     7921 cacttcaagg acgcgcgggg gcaaacccgc gcgggcgctc gcgatctcga accgaggctg
     7981 caacaaattt tcgggccgct cgtttcggtg atcgaagatg cgtctgcgcg tgatcgcgtc
     8041 gttgctgtcc tcggcaagtt gaacgatgac gcggtggagg agcgcagcga gacggccgag
     8101 gcgcaagtct tgagcgtcat ccgccaattg gttgccaagg gtcccgattc acggctggcg
     8161 gtccaggaca tcacctcggc gtttctcgcg cgctatggcc acgaatacga acggcggatc
     8221 acgccgcatt ggatcggctc aatcattcgc aggcgcctcg gccttcgcgc ctcgaaatcg
     8281 acgggaacgt atctcctccg gccggaagat gtgaaacgcc taccggctct ctacgttcgc
     8341 tacggtttga cctccgcgga aaagaacgat ccgggatctc ctgcagtcga ggaaggcgaa
     8401 accaaccacg gcacatcatg agcggagaac cgcttacctt gatcggtgag acgtactggc
     8461 gcgacatgcg gaagcggttc ggcagccggc ccgcggaccg gcgcgcgcac atgtatgtga
     8521 tcgggaagac aggcaccggc aagacctcgc tcctcgaagg catgatccgc caggacatcc
     8581 ttgccgggca cgggctcgcc ctcttcgatc cgcacggcga tctcgcggag cgtctcgcgc
     8641 tctggatgcc ggagtcgcgc aaggcagacc tcatctacct cgatgtgcct gacccaacgc
     8701 agcagttcgg gttcaatcca ctggagggcg tttcgccgtt gcggcgctcc gttgcggcga
     8761 acggcatcgt tgaggctttg aagaaaattt tctcggaggc atggggagtc cgcctcgaat
     8821 atatcctccg cagcgcagtc gtccttcttt tggagcagcc ggaggcgacc ctcgccgaca
     8881 tcgtgcggct cttccatgac aaggactttc ggaaggcggc ggcggagcga tcagagaacg
     8941 agcaggtcaa gcgcttctgg accaccgagt tcgaaaagct aggtcggcag cgctccgagg
     9001 ccgtcgcgcc gatcgagaac aagctcggta gcctgctcgt cgatcccttc gtctcgcgga
     9061 ttcttaccgc gtcgaagagt acgttcaatc cgcgcgcggc gatggacgag ggcaaagtgc
     9121 tcctcgtgaa cctctcgaag ggcaaaatcg gggaggcgcc cgccatgctc ttcggcggac
     9181 ttcttgtcac ggcgctcggc cttgcgggcc tgggacgcgc cgatgtgccg cagaaagagc
     9241 ggcgcgactt cttcatctac atggacgagt tccagacctt cacgaccctg gccctcgcga
     9301 acatgctggc ggagctccgc aagtacggtg tgggtctggt gctcgcgaac cagtacatgg
     9361 accagatcga gccggagatt agaaccacga tccttggaaa cgcgggcacc cttgtcgttt
     9421 tccgcgttgg ggcggttgat gccggaaagc tcgtaaaaga gcttcgcccg gacctcgaac
     9481 cctacgatct cacgttgtta tctaacagga cgttctggat acggccgctc gtgaacgggg
     9541 agtcggtcga ggctttcacc ggaaagacga tcacgctaga ggagtcgaag aattcaggtt
     9601 tgtgaagtac gaaccgaacc tccctataac aaaggaacgc acaacggacg ctattgacag
     9661 taacggaaat atatgcttaa attgtgcccg ttaattctca cacctgcccc tgtcgttttg
     9721 gcctgactta ccagcgctcc ccgcgctgca gaggcctcaa acaacccaaa aagtgatcac
     9781 cgcacgggac ttatgccccc tcggcaggtt ccggatcggc ggttacgcta ctccccaaca
     9841 tgccgtccaa actgatccac tatttacgca atgaacgccg cgagactggc ctcactcaag
     9901 cggatattgc cgcgctctta ggcgcgcctt ggaaatcgaa ggtctcgcgc tacgagcgcc
     9961 aggcggcgtt gcctccgctt gagaccgcgc tcggctacga agcagtaacg cgtaagccag
    10021 tctcccagct cttcgccgga atgtacggca cggtagctcg cagagtaaag gaacgcgcaa
    10081 ggaatcttct cgcaagatca ggcgaggcgc acaccgcgct ccaatctcgt cggaagcggt
    10141 cactcgaacg aatccttcaa tgaaaagact tatcgacccg cgcgcaattc tcgggattga
    10201 cccaaccaaa cgcggccttg cctttgtttt cttcgaaaac ggccgcctca tcgactgggg
    10261 catccggcac acgggaaacg aaatcgccaa cctcgaagcg attctcaagc tctgtcccgc
    10321 ggacgtggtc gttcttgaag atggttctgc ggaacgttgc gaacgccggc cccagatgcg
    10381 taaggtgctg tcgcgcctca cccgtgctgc aaagcaacgc ggcctagaaa ttgtctgcgt
    10441 cagccgcttt gcagtgcgat gcgcgtggcg cgcgcgcgga gtcaccagaa agcacgcggt
    10501 cgcgaaagcg atcgcggagg agtttccagc cttggaagta ttcgttccgc ggccgcgcat
    10561 caaagcctat atggatgaag aggcgcgcgt gcacgtcttt gatgcggcgt cgctcgtcct
    10621 tcacacgttc ggcgtcgctg acggtgattc gctggcagcc tgaatcgagt accgcgctgc
    10681 ggaacttctg ccccctcgtg ggctgcgctc tgcgcccgta gggttagggc agtagcttcc
    10741 caacccacaa ccaaggaaaa caacttcgac aggagaaagg acaccgtcta tgaatccgat
    10801 catcacgctc tcatgccctg actgttcacg aacggtcgag accttccgcc tctccgcctc
    10861 cccgacgacc gtcacgctcc aggcaacctg ccatccctgc aagaccatcg cggaagtaac
    10921 gctcacgttc gaggacttgc gcgacctcgg tgccgggcga tgcgttgccg cgaacttcat
    10981 cgcgagcacg ccggagctcg tcatgtgatg gatctcttag aagaggacgc gctccgagcg
    11041 cagctggcgc gtttccgaag atcagaagag tcctacctcc atccttcaca agctcttgtc
    11101 tacaccgctg gcgtaaggtt cctggctgaa cgctgcggcc ttttctggct catcgatgtt
    11161 attgccgcct ggcaggggca cgccctgaga gatcccgatc tcgcgatttt tcagctctgg
    11221 gaactccggc caattgggac gcggcgcgcg ctggtctgcc tgcgagactc ggagagtgtg
    11281 gcgttcgcgc tgcctgttgg gaatcgcgac tcggcgctcg attacgtgag cgtgtatgtg
    11341 cagttcggcg tcctcatgct gccctcggag aaaagcaagg ctccgcggta ggtgagctgc
    11401 tgctcctcta ttcgcagaaa ttcattcatg catgggacag aacagtccca agaaaggagc
    11461 atgcggcctc gccgaagtcc caagcgttca gtgccggtca cacaagacag taccctcgcg
    11521 catcagcgcg aggttttttc ttccaatagg taccgcggcg cgagacttgt gccccctcgt
    11581 gtgatttttt ccggtgctct aaggtcaggg cacgtacctt cctaattgaa aagtaaattc
    11641 gtggttttcg ggagcgttgc gagtcgccga caaacagtga gagcacttca cagcctcgct
    11701 tgtcctcccg ccttcaggtc cgtgcggctt ggcctctgac gaactcgcgc ttcgacgcga
    11761 gcggtcagcg gcgaagaccc gctgagcacc ttcacaaaag aaaggagaga tgctcatgtc
    11821 tgacctgatt gcgttcaaga gcggcaagga cgatggcgaa gagacgcgca ttgcgcttgt
    11881 cgaaacggtg acctgtgtcg agtgcggcaa agtggagacg gacgagcaag cgtacgacga
    11941 gggttggcag atcgatccgg ttgtgtgtcc cgattgtctc cactggacac tcacccccga
    12001 aggcgagtgt tgcctcggag gcgcgtcatg acggcaatcc gagctgcgcc tcagagacga
    12061 agtaagcgac ggcgtatccg cgcggagttc atgcgcatcc cgtggaacgc gaagcggatc
    12121 gcctgcgata cctgcggcga aaccgaaccc gtcccacccg agctgcttct ccgcttccgg
    12181 ctcaaaacct gcctgagctg cctcacggcg ctcgctcact aacccagcga caaaggagtg
    12241 aacacatgtt ccctgatccg aagggcgtgg cgaagctctg tccgacgcgg ggagccgcga
    12301 cggatagaac accgctcaca accgccctga ttgctcttcc cgccggaggt ccctgatgct
    12361 cttccgagac agctccggca gcttctccta caccagaggc gtgaacggcg tctgtcagat
    12421 ccacggcctc tactggttcc gtgacttcat cgccgcgcag cagaaacgtg tgaagaggga
    12481 cccgcgcttc aggaaggtcc agcgatgggt ccacaaggtc cagttccgac gcgccaccat
    12541 ttactgctac gcgggcgatt ccgacgagac cccgctcatc tttccgcagc acctctactg
    12601 cgacgtcacg gagccagaga tcaccttcta cctcgaaggc gatgtgctga ggacgaagga
    12661 ggagcgcgat gcggtgtgag aagtgcggcg tcgcctacag cgcgatcatc cacatcacac
    12721 gcgtcccttt cgtcggatgg ctctgcatcc cgtgtgcgga agagatcaga gagaaaggag
    12781 ggaacgcgat gctcaggtac gagcgctaca aacgccgctt ctgggccgtg tacgaagacg
    12841 acgggctcct ctgcgtaacg gtctacctca aaggcgcgct ctcagtcata gagcgcatca
    12901 ccggcgtccg gccggagcgc cccgtccgaa agaaacgcgc cccgctgccc ccgatctacc
    12961 gccccaaacc cacacaagaa cgtcagtgga tcatccacga ccgctaacta agaaaggagt
    13021 aattgtcttg aacaatctga accgtgtcat cttgaccggc tacctcggca aagacccgga
    13081 aacggcaccg gcagcatcaa ctaccgttac taagttttcg ctcgccgtca ccgatcgctg
    13141 gaaggatgcc gccggcgaaa gacaggagcg caccaattgg ctgaacgtcg tcgtctggaa
    13201 cggcaacggc gagaacgtcg cgcagtacct gaagaaaggc tcgcacgttc tcatcgaagg
    13261 ctctctccgc acttcggagt gggacgacaa ggacaccggt cagaagcgct ggaagaccga
    13321 gatccatgcg tcccaggtca tcttcctcga taagaaagct gaggagcctc ggaagcgtcg
    13381 caagaaggcg gtctgatgtt cgccgtcgtg agcggcgaaa cgctgcaatg ccctcactgc
    13441 ctctccaccg attctgacca cttcgcagtc gaacgccgag tcctttggcg cgaacccatt
    13501 cttgagatgt ttgaggacaa gggtctcctc gttctcatcg ccgattgcga caacgcgctc
    13561 gtcgatatgg ccggctcagg ccccgactgc ctctactgcg cgaactgcgg caaccgctcc
    13621 gacatcgatg taaccactct cgtccatcac agcgacccaa aagtgtgagg tgtccatgcg
    13681 cttaatcctc atttgtacct gcactcacca aaagcgcgag cactacggca cgaagcagaa
    13741 cccgaacggc ccttgttcgt tctgcacctg tcagaaattc gagcccgagc ccatctgcaa
    13801 atgcggccac ggcaagaaag ctcacgccaa aggtccatgt cacgaaggcg acggatgcaa
    13861 agaattccgt cccgcttaga aaggaggacc gcgaccactt agaagtccca ctcgtgtggc
    13921 taggctgaca aagcctgatt gatctttacc cctcgatccg tttcgggggg cttttctttg
    13981 ccgtccacag cccgcccatt tttcgctcgc ctggcggttt tctgtgtctg gtccgaaaga
    14041 aagtcggcaa aactgcacac gctcgtgtgc aggctgattt gacccttctc cgttccgagc
    14101 tacggtccta cgcaacatgg actacgcgca ggttctactg cacgccgagt tcgaacacgg
    14161 caaaccgctt ggcgataccg ctgccgtcgt cagccttcct ccgaaagtga tcgacgacca
    14221 cgagatcgtc taccgcgtga gacgagcgcc cgatatgaag cgtggcgatc tcctcgtgat
    14281 tgagccccgc tccacggccg caacgggcga gctcgtcgtg gccactcgcg gaaagaacgc
    14341 ctacgtcggc cattggtggg cgaaacacgg ccgtaaggaa ctcctcgaac acccgaacaa
    14401 acacttgacc ggcgagttcc aaatcatcgg cgcggtcaat ctcatcgtga ggctctcatg
    14461 accaagctcg aagcattcat caagtcccgc aagttgaaac ccattcggat cgcgttcgag
    14521 gccgactact cccggcagca cttgctccgc atccggaaag ggcaaatgga acctacccgg
    14581 aagtgcatca aagccatcac cgtcgctatc cgaagactca cacgcgaacc tattaccgcc
    14641 tctgacctct tcgacctgga cgaggagacc taacgaatga gaaagaccct aaggctccga
    14701 ctcatcaccg ccacgcagtt cgagcaattc tttctgctgg actctccggg ttacttcatc
    14761 gcacgggatg ccatgaacgc cggcgaaacc gccacagcaa ccgatgcgct cgaagtgctc
    14821 tgtctcgcga accaactcta cgaaagcgag cttcttaacc gtctcatcgt cgagctcgcg
    14881 accctcggca agctggacga gtacatcgaa aagcgcggca cagacgaagg tggagaattc
    14941 atcgtcgaag tcttcggaaa ggagaaggcg ctccgcattc tccaggacgt tacgaacgaa
    15001 gtgaagcgtc agagcaaggt cggcacgggc gagaacgaca aagctgtgag ccggtaagcg
    15061 ggatggccga aaccaccccg ctacaaacac tattctttgt tcggccgatc gtcggcttct
    15121 tgatccttca cttcaaagaa gaacgaaccc ggacctagcc tcgcgccagc tcgcacgttg
    15181 gccctccggt tcaatgccgt atttgttagt ttgtaaaaaa tgagaagccc tatgatgagc
    15241 agaattagga aggcgatcgt catgctcatc gccttactcc gctggtcgcc gctaaagtcg
    15301 ttaaggtttc gcctacctct tgcgcccgcc tcgtttctac ctcaagctca ctcgtgacct
    15361 tatcggcttc ctcaacgagc gcttccaaga gtttcggaat ctcttttacg gctcggattc
    15421 tcaattgtct cggcgcatca ttgaaagccc aggacgttct tgatccatag tccggatcag
    15481 acgcatgcac gttaccgctt tcttccgcaa gcgcgataca ccacttcccc cccattttcg
    15541 cgtagccgat ttccagctga tggtagtcgc cgtcttggtc tggaaagggt ccaaagaata
    15601 cgtgccactt cgcgatgccg aggttcagtt ttttgaacac agcctcgatc gggccgatag
    15661 ctctcgacaa ctcatccgac gctttattca gacggttggc ggactctgca agacgttgga
    15721 aggattggga gagatcctgg ggtggggtgg gcgtagcttt gcctggattg gccatacatg
    15781 cctttcggac cattcggtct catgctgcat agttttgagc ttgtcagctc gttcatgttt
    15841 ccatcctgcg atggggtcga taccgtgtcg gctttcaccg ttagctgagt acacctcagc
    15901 aatcgaaccg ctctgattat acgccctacg ccgtaaggac tggcctaaat aaggtaaaac
    15961 cgcccatttc tggacgattt cttaccggtt ctgaagaaga tcaggctgtt ttcacgagcg
    16021 caagttgcgg cttcggctca acccagagag aacggacggc tgtttcgagt gccacctgcc
    16081 tcgatttcac aaacggggcg tagtgcttaa taactatcgc cggcgagttc ccgaggatcg
    16141 ccgctaccgt ctcaactgcg attcccttgt tcagcaagtc caccgcgagc gttgtgcgga
    16201 acgagtgcgc cgacccgtgg ttcacctcag ccaaagtgag aaccgttcga atcgtccggt
    16261 cccatccgcc gcggacggtc ttcggcttcg ccttgccggt ctggaagtaa tactcactga
    16321 ccctaggaac tgcggctagg gcatctacga cgaactgagg aatcggaatc cacacgagcg
    16381 ttcccgtctt ctcggtccgc agcagaacct tcccatctct cacatccgat accctgagac
    16441 tggcggcgtc agagatgcgc aggccggtgt atcggaggat gtagatgaac gcccggaccc
    16501 gcttcacgtt ctctttgccg taggttcccc gcgtgatgag ccggccgcac gcctcgaaga
    16561 ttttcggtag ctcctcctcg gagaagggtt ccttgccgac ggtccggacc tccggcggct
    16621 tcagagcctt cgcggggttc ttctgaatcc agccggtatc caggcagaag ccgaaaaaga
    16681 ttttgagccg gtcgattctt cgcgccgcgg tcaccggttt gcacgtccaa gcctcgcggt
    16741 acgtccgcag tttctcgagg tccagctcgg ggagagcttt cacgccttcc gcttcgcacc
    16801 acgaaaggag atgtttcagg cctcggcggt agagatcaac cgtacttgct ttgagccggg
    16861 tttcagcgtc gcgcaggtac ttcgtaacag cgtcctcgac gctcacggcg cgcctacctt
    16921 cggcgccgat ctttcccgct tccttccaag cctgaacaac tcgctcggcg gaatcccacg
    16981 aagtgaggtc gagagattta cggatttctt cgccggcaag tgtgccctgc acccatatgg
    17041 ggcactggca gcggcgatac cgcctagagg tttgaccgca cgttgaagtg tgtctgcgat
    17101 agagagccaa catgtccgcc tccgattctc ggtcgaaggg gacgtaattg gtacagctcc
    17161 gaagctccag aatctaactg cctctcggga aggcagttaa ggtggtcggg gcaagaggat
    17221 ttgaacctcc gacctcacgg tcccgaaccg tgcgctctaa ccaggctgag ctatgccccg
    17281 gggaagtcgg gacgcgccgg gaacggcgcg aaaaagagtg cggattctag gagatccgcg
    17341 ccgaccggtc aaaccgggct atcccgaggt tcattccgcg ccgcgatagg agctcttccg
    17401 atcagaaccg ccacgaaagt ccggtcgaaa caatcagagg gcgaacgttc attttgaccg
    17461 acgacgaagt gccgatgaag gccgcctccg acttcgtttc gatcgggatg tagcgggcat
    17521 caccgaggat gctccaacgc ttggatagct gcacttccag tccgccgtcg acgacgattc
    17581 ccgtcgggtc tttgaagtgg atgccgctgg ctcctgtgct tcccaccgag tgattgatgt
    17641 tgcgcagaat gacgtgcgcg acgccggcgc cgaggtacgg acggatcgtg ccgtgttcgg
    17701 tgagatgcca ctgcagcaac gcggtgatcg ggtaaatctg agcacggccg agatccgcga
    17761 ccgcgaagaa gtcggagccg atcgttcgga ctttgagctg cgattccagc gcagcggctt
    17821 tggcctcgat cgatacgcgg tcggtcagaa agaaatttaa tccgaggccg gcgccgccac
    17881 cggttccgaa ctccgggtcg aaatggacgc cggtcgagaa gtctgcgttc cgtccgggac
    17941 gccgcgcgcc ctcggcatct accagaacat ccacccgatg ctgcgcgagc agcggcgcag
    18001 cgaagagcgc gatgagaatg acgagcagcc gtcctttcat tgcggtgaac aagcagcaag
    18061 aaccgtgcgc tgagctcgat tcgaggaatc ccctctcgcc gcaacctcgt taggtatcgc
    18121 cgaaggggga ctcatgtcca tgcgtttctt cccggtgctc attcttgctg ccgcaccgct
    18181 tttcgcgcag cagcctccgc cgccaccaca accttcacca accttcggcg agaaggtcga
    18241 tgtcaatctc gtccagctcg acgcggtggt caccgataac cgcggtaacc agatgctcgg
    18301 actgacgaaa gacgatttca tcgttcgcga aaacggcgtg cagcagacga tcgacacggt
    18361 cgactacttc acgaaccgcc gactcatcga tgcccccgag gcgttggcga agttcaaagt
    18421 cgagcgcgtt cacgacgagc gctacctcat
//
LOCUS       DATLVW010000186         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_176189, whole genome shotgun sequence.
ACCESSION   DATLVW010000186 DATLVW010000000
VERSION     DATLVW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176189"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1122
                     /gene="ftsW"
                     /locus_tag="VNN25_06005"
     CDS             22..1122
                     /gene="ftsW"
                     /locus_tag="VNN25_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXH91114.1"
                     /translation="MSRKLTYDTYLFGAAMLIVVVGMVMIYSASAIITMQKVGSDNPY
                     YFITRQLVWLIAGSAVMVTLMHVDAARLRDRRIIYAIVGAVLVALVIALFQTPINGTH
                     RWIVVLHRFQLQPSEFAKPAIVLFLASYLARKEERINEFTSTLLPLAFVVALFAVLIL
                     LEPDFGTASTLVLVAAGMIFAAGVSWMRVVTFTLMIIPAGLLLVLSAAYRRDRLFTFL
                     NPEADPLGKGFQAMQSLIAIGTGGLRGLGIGNGRQKLFFLPEPHTDFIFSIIGEELGF
                     IGAFALVAIFAFLVWRGFRVTRFSQNRFDFYAALGFTLMIGIQALLNVSVALCLLPTK
                     GLPLPLVSYGGSSLITSLIAVGILLNLSQQSG"
     gene            1135..>2145
                     /gene="murG"
                     /locus_tag="VNN25_06010"
     CDS             1135..>2145
                     /gene="murG"
                     /locus_tag="VNN25_06010"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXH91115.1"
                     /translation="MGVQGVRGMLMIAGGGTGGHIYPAIAIAQEWMARGPARRVVFVG
                     TERGLEKTIVPKAGFPLALISVGGLKGKGPGDTIRNLFRLPRGFVQAFSLIGKHRPNV
                     VLGVGGYSSGPVLLAARLRGTPTAIHESNAFPGLTNRLLARVVTAAAVAFEVAATRMK
                     RPDAVVTGNPIRKEFFDAGSGMRPAASDSRKRLLIFGGSQGSRTINDAMIGALLFLAP
                     LKERIDIVHQTGPADLEKVQAGYRASAFPDARVVPYLDPIVAEIAAADLAVARSGAMT
                     VGELAAEGRASILIPFAAATDNHQELNARAVEEAGGAIVITERELSPERLAFAITGMI
                     SDP"
BASE COUNT          343 a          701 c          670 g          431 t
ORIGIN      
        1 cacccgaccc ccggaaaaac catgagccgc aaactcacct acgacaccta cctcttcggc
       61 gcggcgatgc tgatcgtcgt cgtcggtatg gtgatgatct actcggcgtc cgcgatcatc
      121 acgatgcaga aggtgggatc ggacaatccc tactacttca tcacgcgcca gctcgtgtgg
      181 ctcatcgcgg gaagcgctgt gatggtcacg ctgatgcacg tcgatgcggc gcggctgcgc
      241 gaccggcgca tcatctacgc catcgtcggt gcggtgctcg tggcgctggt catcgccctc
      301 ttccagacac cgatcaacgg cacgcatcgc tggatcgtcg tgctgcaccg ctttcaactg
      361 cagccgagcg agtttgcgaa gccggcgatc gttctcttcc tcgcctcgta ccttgcgcgc
      421 aaagaagagc gcatcaacga attcacctca acactgctgc cgctggcatt tgtcgttgcg
      481 ctcttcgcag tactgatcct gctcgagccc gacttcggca ccgcgtcgac gctcgtgctc
      541 gtcgcggccg gaatgatctt cgcggccggg gtttcgtgga tgcgcgtggt gacgttcact
      601 ctgatgatca ttcccgcggg cctgctcctg gttctgagcg ctgcctaccg gcgcgaccgg
      661 cttttcacgt tcctgaaccc ggaagcggat ccgctgggaa aagggttcca ggcgatgcag
      721 tcgttgatcg cgatcggcac cggaggattg cgcgggctgg gcatcggcaa cggccggcag
      781 aaactctttt tcctgccgga gccgcacacc gacttcatct tctcgatcat cggcgaggag
      841 ctcggcttca tcggcgcctt cgcgctggtg gccatcttcg cgtttctcgt ctggcgcggt
      901 tttcgtgtga cgcgcttcag ccagaaccgc ttcgatttct acgcggcgct cggcttcacg
      961 ctgatgatcg gcatccaggc gctgctcaat gtcagcgtcg ctctttgtct gttgccgacg
     1021 aaagggttgc cgctgccgct cgtcagctac ggcggatcgt cgctgatcac cagtctcatc
     1081 gcagtcggaa tcttattgaa cttgtcacag caatcgggct gaggaaattc atccatgggc
     1141 gtgcagggtg tgcggggaat gctgatgatc gccgggggcg ggacgggggg gcacatctac
     1201 ccggccatcg ccatcgcgca ggaatggatg gcgcgcggtc ctgcacggcg cgtggtcttc
     1261 gtcggcaccg agcgcggtct ggagaagacg atcgttccga aggccggctt cccgctcgcg
     1321 ctcatctccg tcggcggcct caaaggcaaa ggcccgggcg acacgattcg caacctcttc
     1381 cgcctgccgc gcggcttcgt gcaggccttc tcgctcatcg gcaagcaccg ccccaacgtg
     1441 gtcctcggcg tcggcggtta ctcgtccggg ccggttctgc ttgccgcgag acttcgcgga
     1501 acgccgactg cgattcatga gtcgaacgcc tttcccggcc tgaccaatcg gctgctcgcg
     1561 cgcgtcgtta ccgcggccgc agtggcgttc gaagtggcgg cgacacggat gaaacgtccc
     1621 gacgccgtcg tcaccggcaa tccaatccgc aaagaattct tcgacgccgg ctccggcatg
     1681 cgccccgcgg cttccgactc ccgcaagcgt ctcctgatct tcggcggctc gcagggatcg
     1741 cgaacgatca acgacgcgat gatcggagcg ctgctgttcc tcgcgcctct caaagagcgc
     1801 atcgacatcg ttcatcagac aggtccggcg gatctggaaa aagttcaggc cggatatcgg
     1861 gcgtcggcgt ttcccgacgc gcgggtcgtg ccgtatctcg atccgatcgt cgcggagatt
     1921 gcggcggcgg atcttgccgt tgcgcgctcc ggcgcgatga cggtcggcga gctcgccgcg
     1981 gaggggcggg cctcgattct cattccgttc gctgcagcaa cggacaacca ccaggagctg
     2041 aacgcacgcg ccgtcgagga ggccggcggt gcgatcgtca tcacggaacg cgagctttcg
     2101 ccggagcggc tggcgtttgc gatcacagga atgatcagcg atccc
//
LOCUS       DATLVW010000187         2101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_419002, whole genome shotgun sequence.
ACCESSION   DATLVW010000187 DATLVW010000000
VERSION     DATLVW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419002"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VNN25_06015"
     CDS             complement(<1..411)
                     /locus_tag="VNN25_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91116.1"
                     /translation="MSENYTRLLPALKERLPASELADHGRTTGFIQRLRTIGASDFVW
                     AVVMSRFATGIPGFDQARRIYAQLSGRDIWQRPFQKRFLSEAAVKLFAAAFEGAVAPW
                     RKRRRIDHPLAKHFSDIVAIDSTVVQLNDRLRRKF"
     gene            complement(702..1952)
                     /locus_tag="VNN25_06020"
     CDS             complement(702..1952)
                     /locus_tag="VNN25_06020"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXH91117.1"
                     /translation="MDIVIARCCGLDVHQGSITACVLLDGRQRPRKLIRTFRTMRAEL
                     EELRDWLKSLAVTHAAFEGTGVYWMPLYSVLEGHFDVSVVNARHVKQLPGRKTDVNDS
                     EWLATLLRHGLLRKSFIPSREIRALRDMTRYRRMLVQSATAEKNRLVKLLEMAGIKLS
                     SVLSDVFGKSGMDMIRALAAGNVALEGIVQMARKRLRKKLPDLRLALDVLIDEHHRVM
                     LRDQLARLDRTSEEIAHYDGLLAELAKPYEEHLALLCSIDGIQRIAAIEIFAEVGPNL
                     SSFPSDGHFSSWAGTCPGQHESAGKHRKTRRRRGNPYLQSILVECALAATRKKGTYLK
                     DKYHRLRARRGTMRALFAVAHKLCRAVYRVLTSKTPHEDLGDAYLDRRNSKAVARNLV
                     ARLQRLGFDAAAVAGLFTQTTPST"
BASE COUNT          410 a          633 c          625 g          433 t
ORIGIN      
        1 gaatttccgc cgcaggcgat cattcagctg aacaaccgtc gaatcgatcg cgacgatatc
       61 tgaaaagtgc ttcgcgagcg gatggtcgat gcggcgtcgc ttccgccatg gcgcaaccgc
      121 gccttcgaaa gcggcggcaa acagcttcac ggcagcctcc gacaggaatc gcttctggaa
      181 cggcctctgc cagatatccc gaccgctcag ctgagcgtaa atccgtctgg cctggtcaaa
      241 gccaggaatt cccgtggcaa atcggctcat gacgaccgcc cagacgaagt cgctcgcacc
      301 tatcgtcctc aggcgctgaa tgaatcccgt cgtcctcccg tggtcggcca gctcacttgc
      361 gggcaggcgc tccttgagcg caggcagcag cctcgtgtag ttttcactca tcggagtccc
      421 tcgcttcgtc cgcaattctg cgggcgatgt cgttgttgaa cggggactcc gatacctatt
      481 tttgcgcccc cttgtcgatc ccctgcgctc taaagatcat ccgcatgcct cggtggtaca
      541 acgcaaactt ttcatcgaga cccctcgtgt ttttcacgtg gcgcgagacg cagcagaatt
      601 ggaacgacga ttcgacgtga gtctgcgccg ttctgacaaa aaaccttcct tccttccgcc
      661 ctcctacgaa aacagagacg taagtaggcg gacggactgg cttacgtgga aggggtcgtc
      721 tgagtgaaga ggccggcgac cgctgccgcg tcgaacccaa ggcgctgcag gcgggcgacg
      781 aggttgcggg cgacggcctt ggagttgcga cggtcgaggt aagcgtcacc cagatcctca
      841 tggggagtct tgctggtgag gacgcgatac acggcgcggc agagcttgtg cgcgacggcg
      901 aagagggcgc gcatcgttcc gcggcgggca cggaggcggt ggtatttgtc tttcaggtag
      961 gtcccctttt tcctcgtcgc ggcgagggcg cactcgacga ggatggattg caggtagggg
     1021 ttgcctcgcc gtctccgcgt cttccgatgt ttgccggcac tctcatgctg gccgggacag
     1081 gtgccagccc acgaagagaa gtgcccgtcg gaagggaaac tcgataggtt gggcccgact
     1141 tcggcaaata tctcgatggc ggcaatccgc tggatgccat cgatcgagca aagcagagcg
     1201 aggtgctcct cgtacggttt tgcaagctcc gcaagcaagc catcgtagtg cgcgatctcc
     1261 tcggaggtcc ggtccagacg agcgagttga tcacgaagca tgacgcgatg gtgctcgtcg
     1321 atgaggacat cgagtgcgag gcgcagatct ggcaacttct ttcgtagacg ctttcgagcc
     1381 atctggacga tgccttcgag agccacgttg cccgcagcga gcgcgcggat catgtccatt
     1441 ccagactttc cgaagacgtc actgagtacg ctactgagct taattcctgc catctcgagc
     1501 agtttgacca gacgattctt ctcggcagtg gcgctttgca cgagcattct ccgataacgg
     1561 gtcatgtcgc gcagagcccg gatctccctc gacggaataa agctcttgcg aagcaaccca
     1621 tgacgcagaa gcgttgccaa ccactcagaa tcgttgacgt cggtcttacg ccccggcagc
     1681 tgtttgacgt ggcgggcatt gacgacgctc acgtcgaagt gcccttcgag taccgagtag
     1741 agtggcatcc agtagacgcc ggtcccctcg aacgcggcgt gcgttactgc gagtgatttg
     1801 agccagtccc ggagctcttc gagctctgcc cgcattgtcc ggaatgtccg gattaatttc
     1861 ctcggccgtt gccttccgtc gagcagtacg caggccgtga tggacccctg gtgcacatcc
     1921 aggccgcaac aacgagctat gacaatatcc atttcgcacc tccgatcgaa gaccgaaggg
     1981 ccgccacgct tccaatgctg agattctcct acacgccctt cctctattga ggtccggcaa
     2041 acttgtggta gctcgaagaa gcgcgggtca gtcttcctga cggggtagcc caccacgtgc
     2101 g
//
LOCUS       DATLVW010000188        12294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_176254, whole genome shotgun sequence.
ACCESSION   DATLVW010000188 DATLVW010000000
VERSION     DATLVW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176254"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VNN25_06025"
     CDS             complement(<1..594)
                     /locus_tag="VNN25_06025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH91118.1"
                     /translation="MLTDRSGPMSSLPVLVKAIVLTFLIAMPALAGQDPRIAEGTALH
                     DAGKYDEAIAKYLAILADDPSNGLASYELAYSYQAKGDYAKCIGVLKPLSAAESVLLP
                     KILASLGNCLDSSGDAPGAVAAYRKSLALVPDDPSTLYNLGVTLVGLSKLDEARDVLK
                     KELIARPGHASGHYALARVFDAQNFRAAAILEYLRFLA"
     gene            complement(767..3256)
                     /locus_tag="VNN25_06030"
     CDS             complement(767..3256)
                     /locus_tag="VNN25_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXH91119.1"
                     /translation="MTFTHKYVAWLLVPPAVVMGPLSFLFLTQIVRMSLATSGSIFGL
                     FALFFLAGSATLVRGLTPLANAVDEDVRRGKDASEAASRCLERTETLTLIVWGAGSIL
                     YAILASFLVMPTILGLGYFLVSALIAAFPSAIWAYAVGKRLIAEHASHGGALRYTGRR
                     IPLGRKIAIVFIGSFLISFAALVAMISSKVSATLENLAISSASDRFQRVYDSANLAAK
                     VDPSIVDTLRLYVPSDYAIALIPRQGRITTSLPDALTPAEVEAIRQIGNGDSSAFVSP
                     HVCRFARLKDGSIIVLTVPWEPYKNIPQQITFYLVVIALVTLAAFIAAALFLSRDVAR
                     PVRAIRGLAADMAQGDFNTMARVFSDDEVGELADSFGETRANLRRLLGRVGGSGSTIT
                     AGVRVITGGTESLLLRSADQAALTENSSHAVEIVRGGIGGVLGAADTVTDLTQDASSR
                     ALELRASSEEVARSMDYLFQSVEKTSSSTTQMNAAMTEMSKRTDVLAGIGEEALSFVS
                     EMESTIVELRASAQSTADISRQVREDAEAGGSAVGKTVEGINLSRDVTNSTAETLDEL
                     QRSVGQISQILNVIEEITNRTNLLALNAAIIAAQAGEHGLGFTVVADEIRQLAERTRG
                     STKEISAIVKAVQGGSKQAAAKMKEGVKRVEATVQLADDASASLVKIVGSASQSYEMA
                     TKISRALDDQAKASRHLHEVTSRMSDHIAEINRGSREQARGTQLLAQEAERVREIAAQ
                     VKTATDEQSQAGRGISSALEKIAEDARAMRDSLDRQLKETDRIADASRTMLDIAQAND
                     AIAREFNATVQSLVTSGRDFESEVARFRYSD"
     gene            complement(3349..5112)
                     /gene="treZ"
                     /locus_tag="VNN25_06035"
     CDS             complement(3349..5112)
                     /gene="treZ"
                     /locus_tag="VNN25_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXH91120.1"
                     /translation="MCEKCGLGAIVRDDGRCEFRVWAPQVEKVELHIVAPDDRRIELA
                     TNLRGYHEVVVDAGPGTRYFFVINGRERPDPASRSQPDGVHEASEVVASAFDWHDAGW
                     RGLLLEDYVIYELHTGTFTDEGTFDAVIDHLDDLKNLGITALELMPIGQFPGTRNWGY
                     DGVYIGAAQNSYGGAGGLKRLVDACHARGLALLLDVVYNHLGPEGNYLSEYGPYFTDR
                     YKTPWGDALNFDGPHSDDVRWFFIHNALQWIDEFHIDGLRVDAVHAIADRTAEPFLQD
                     LTTAVRERGDALGRRVYTFAESNLNDPRVITPRDDLGLGFDAQWSDDFHHSLRTLLTG
                     ETSGYYAGYGRTSDLARTLASGYLYTGQHSVYRGRKHGVAPKTRDGKQFVVFSQNHDQ
                     IGNRMTGDRLAAEVSLSKVRIAAAAVILAPFIPMLFMGEEYGETAPFQYFTSHADRDL
                     IEAVRRGRHKEFARFQWSEEPPDPHDSDTFLRSKLRREPLDDIRALYKELLRLRATTP
                     ALRQLDLDAVETHADDEQRTLLIKRRNTLLAFNFSDAPRVIGLPFDDAVWRAMIETDA
                     GINGNVLTMAAESFGLFQTEE"
     gene            complement(5480..7738)
                     /gene="glgB"
                     /locus_tag="VNN25_06040"
     CDS             complement(5480..7738)
                     /gene="glgB"
                     /locus_tag="VNN25_06040"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXH91121.1"
                     /translation="MDGKETEGDAPAERTDPITDVTPEADAPSKKKASPRKRATKTKA
                     DAPKKAKAAPRKRAPKVNRVTEVAQAVGADVVPHVTIEVDSPRKEHVEVTATDSAKPG
                     DSLLSDWDYHLFNEGSHQRLWEKLGAHPHTRDGVPGTMFAVWAPNADAVSVIGDWNGW
                     GPDANPLENRGPSGIWEGFVPGVGKGVHYKFHIRSRFNNYRVDKADPFGVMHEMPPRT
                     ASVVWDLDYEWNDGAWMEQRAARNAFSAPMSIYEMHIGSWRRTPGDDGVERPLTYREL
                     ARPLADYLRTMNFTHVELLPVMEHPFYGSWGYQCTGYFAPTSRYGTPQDLMYLIDVLH
                     QHDIGVILDWVPSHFPTDEHGLSYFDGTHLYEHADPRKGFQPDWGSLVFNLGRREVQS
                     FLLSSAIYWLGVYHADGLRVDAVASMLYLDYSRKHGEWIPNEHGGRENLEAIAFLRRL
                     NEDVYEAYPDVQVIAEESTSWPMVSRPTYIGGLGFGMKWDMGWMHDTLRYFALDSVHR
                     KFHHNDLTFRMMYAFAENFLLPLSHDEVVHGKGSLIRKMPGDEWRRFANLRLLFAHMY
                     SQPAKKLIFMGGEFGQSREWNHDAALDWNELESLPNETLQRWVEDLNKAYRDIPAMHE
                     LDLSPDGFEWIDCCDTENSILVLLRKSSSQPEQPVAIALNFTPLPRYNYMIGVPIGGH
                     WSEVLNSDAKVYGGSGQGNMGGIDAAPIPLHGRRWSLNLTLPPLGAVFLMPDGEGDLE
                     EAAAELALAFAG"
     gene            7912..8319
                     /locus_tag="VNN25_06045"
     CDS             7912..8319
                     /locus_tag="VNN25_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91122.1"
                     /translation="MANGNVLVVEDDEAVRRLLIDYLHLNHAIDVDGARDGIEALHQI
                     VTNRYSVMILDLMMPKMSGVDLLDSLEALISDPSVKALDHLPAVFVITSFATAALPDD
                     VIHQRCPQAVQGIFRKPLDIEKVSASVGRYLDA"
     gene            complement(8477..10633)
                     /locus_tag="VNN25_06050"
     CDS             complement(8477..10633)
                     /locus_tag="VNN25_06050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HXH91123.1"
                     /translation="MKIAAAVILLAATSLFAQQNPDPVKNARPAETPKPSAAKPSDAA
                     PPPKESPVPAAPAPKEHPLSALPYTPSLDIPSMDRTANPCADFYQYTCGGWMKNNPIP
                     GDQASWSVYGKVTEENEEYLWGILLDAAKPRADRTAVQQKIGDFFDACTDEAHVEELG
                     AKPLQPLLDEIAGLKAKYELGGFLARQHMRSNGNGWIFGFGSTQDYGDASSVIAEASA
                     GGLGLPDRDYYTKTDAKSKEIREKYVAHVAAMLELIGESKAEAKKDAATVMHIETVLA
                     TASLTRVEKRNPYNLAHKMTVADFSKIAPAFEWKSYLVESGVISNVQTLNVTEPKFFA
                     AVEKEIKSVPLADWKAYLRWHAVHARAPYLSKKFLEEDFNFYRKTLRGVKSMPPRWKR
                     CVQLVDRDLGEALGQEFVARTFTPETKAKTVDMTKRIETAMEDEIKNLDWMTPATKER
                     ALEKLHAVANKVGYPDRWRDYSTLKVVRGDFYGNTTRATLFESQRDLAKIGKPVDRNE
                     WGMTPPTVNAYYNPQMNDINFPAGVLQPPLYDAKLDDAPNYGNTGSTIGHELTHAFDD
                     EGRQFDAKGNLKDWWTPDDAKKFEDRVNCVRDQYAGYTIVDDIKINSKLTSGEDVADL
                     GGTLLAYIAWKAADPNDTQPIDGFTPDQRFFIGFAQWACENQRPENLRANAITNPHSP
                     GKYRINGIVSDLPQFQKAFNCPADAPMVNKKACQVW"
     gene            10818..11147
                     /locus_tag="VNN25_06055"
     CDS             10818..11147
                     /locus_tag="VNN25_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91124.1"
                     /translation="MLGFALTMTCSAPTMLGVALTMMCSAPPLPCFSPPILHNSPPML
                     HNFPTMLHDYPPMLLNYPPDAAQLPPDAAQLTPDAAQLPHDAAQLPHDAAQLTPEAVF
                     RTIDGAF"
     gene            11345..>12294
                     /locus_tag="VNN25_06060"
     CDS             11345..>12294
                     /locus_tag="VNN25_06060"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HXH91125.1"
                     /translation="MADLSTTFTGIRFENPFLLASAPPTESDANIIRAFEAGWGGVVT
                     KTIGLHPVVNVKGPKTKFLRSEDQGTRISMTKRPGTTVMASWNWELISDKPLEWWLGK
                     LAAIKKAYPKHIIVGSIMAGSGNDKELGNWQTLARALQDEGADALELNFSCPHMDRVD
                     MGSNVGKDKVLCSVVTQAVKQVSKIPVWVKLTPATANIVEEAEACFRGGADAVVSSNT
                     FPSLPVIDPATLEFEMNVDGYVSSGGLGGPAILPLSLAKMSQLTREFPDKEFSGIGGI
                     ANFDHALNYFLLGCGTTQVCTAAMLDHAIGPNVIKALTEG"
BASE COUNT         2193 a         3927 c         3811 g         2363 t
ORIGIN      
        1 ggccaggaag cggagatatt cgaggatcgc cgcggcgcgg aaattctgcg cgtcgaacac
       61 acgggctaaa gcgtagtggc cgctggcgtg accggggcgt gcgatgagct ccttcttcag
      121 aacatcgcgg gcttcgtcaa gcttcgacag gccaacgagc gtgacgccga ggttgtacag
      181 cgttgatgga tcatcgggaa cgagagcgag ggatttgcga tacgcggcca ccgcgccggg
      241 cgcgtcgccg gagctgtcaa gacaattgcc gaggcttgcc agaatcttgg ggagcagtac
      301 cgactcggct gcggacagtg gcttcaagac cccgatgcac ttcgcgtaat caccctttgc
      361 ttgatacgaa tacgccagct cgtaggaggc caagccattc gaaggatcat cggcaagtat
      421 cgccaggtac ttggcgatgg cctcgtcgta tttgccagca tcgtgaagcg cggttccttc
      481 ggcgatacgc ggatcctggc cggcgagcgc gggcatcgcg atcaagaacg ttaggacgat
      541 cgccttgacg agcaccggca gagaggacat cgggccggag cgatcggtca gcatgaggtt
      601 gagtttacct gtgttcggaa tgctttatgg aaagggagtc tgctcgttct ccccgttcct
      661 ttgacccact accgtacacg actacgattc agacaccaaa gtgctgccac ggtgacctcg
      721 atagacgcgc gcaccgacta ctgccagact ctagacctaa ccccagctag tcgctgtacc
      781 ggaaccttgc cacctcggat tcgaaatcgc ggccgctggt gaccaggctc tgcaccgtgg
      841 cgttgaactc gcgggcgatg gcgtcgttcg cctgggcgat gtcgagcatc gttctcgacg
      901 cgtcggcgat gcggtcggtc tccttcagtt ggcgatcgag cgaatcgcgc atggcgcgcg
      961 catcctcggc gatcttttcc agtgccgacg agatgccgcg gccggcttgc gactgctcgt
     1021 cggtcgccgt cttcacctgt gcggcgatct cgcggacgcg ctccgcttcc tgcgcgagca
     1081 gctgcgtgcc tcgggcctgt tcgcgcgagc cgcgattgat ctcggcgatg tgatcggaca
     1141 tccgcgacgt gacctcgtgc agatggcggc tggctttggc ctgatcgtcg agcgcgcgag
     1201 aaatcttcgt ggccatctcg tacgattgcg atgcgctgcc gacgatcttg accagggacg
     1261 ccgaggcatc gtcggccagt tgaacggtgg cttcgacgcg cttcacccct tccttcatct
     1321 tcgccgcggc ctgcttcgat ccaccctgca ccgccttcac gatcgcgctg atctccttcg
     1381 tcgatccgcg ggtgcgctcg gcgagctggc ggatctcatc cgcgacgacg gtgaagccca
     1441 gcccgtgctc gcccgcctgc gcggcgatga tcgcggcgtt gagggccagc agattcgtcc
     1501 ggttggtgat ctcttcgatg acgttgagga tctggctgat ctgaccgacg ctgcgttgca
     1561 gctcatcgag cgtctcggcg gtgctgttcg tgacgtcccg cgagagattg attccttcga
     1621 cggtcttgcc gacggcgctg cctcccgctt ccgcatcctc gcgcacctgg cgcgagatgt
     1681 cggcggtcga ttgcgcgctg gcccgcagct cgacgatggt cgactccatc tcagagacga
     1741 acgagagggc ctcctcgccg atgccggcca gaacgtcggt gcgcttcgac atctccgtca
     1801 tcgccgcatt catctgcgtc gtcgacgaag aagtcttctc caccgactgg aagagatagt
     1861 ccatgctccg cgcgacctcc tccgacgacg cgcgcaattc gagggcgcgg ctggaggcat
     1921 cctgcgtgag atcggtgacc gtgtctgcgg caccgagaac gccgccgatg ccgccccgga
     1981 cgatctcgac ggcgtgcgac gagttctcgg tcagcgcggc ctgatcggcg gagcgaagga
     2041 gtagcgactc ggtgccgccg gtgatcaccc gaacgccggc cgtaatcgtc gagccgcttc
     2101 cgccgacacg gccgaggagc cggcgcaggt tcgcgcgggt ctcgccgaag ctgtcggcaa
     2161 gctcgccgac ttcgtcatcg gagaagacgc gcgccatggt gttgaagtcg ccctgcgcca
     2221 tgtcggcggc cagcccgcgg atggcgcgca cgggacgtgc cacgtcccgc gagaggaaga
     2281 gcgccgcggc gatgaacgcc gccagcgtga ccagcgcgat cacgaccagg tagaaagtga
     2341 tctgttgcgg aatgttcttg tagggctccc acggcaccgt gagtacgatg atcgagccgt
     2401 ctttcagcct ggcgaaacgg cagacgtgcg gcgagacgaa ggccgagctg tcgccgtttc
     2461 cgatctggcg aatggcctcg acttcggcgg gcgtgagtgc gtccggcagg ctggtcgtga
     2521 tccgaccctg ccgcgggatg agcgcgatcg cgtagtcaga cggcacatac aaacggagcg
     2581 tgtcgacgat cgaaggatcg acctttgcgg cgaggtttgc gctgtcgtaa acgcgctgaa
     2641 agcggtcgga ggcggaggag atggccagat tttcgagcgt cgccgacacc ttcgaggaaa
     2701 tcatcgccac gagcgctgcg aacgagatca ggaacgatcc gatgaagacg atggcgatct
     2761 tgcgcccgag ggggatgcgc cggccggtgt atcgcaaagc accgccgtgc gaggcgtgtt
     2821 cggcgatcag ccgcttgccc acggcgtacg cccagatcgc ggaagggaac gccgcgatga
     2881 gcgccgagac gagaaagtag ccgagcccga ggatcgtcgg catcaccaga aacgacgcga
     2941 ggatggcgta gaggatgctg cccgcacccc agacgatcag cgtgagcgtc tcggtccgct
     3001 ccaggcagcg cgatgccgcc tcggaggcgt ctttcccgcg gcgcacgtct tcgtcgaccg
     3061 cgttcgccaa cggcgtcaaa ccgcgcacga gcgtcgcgct gccggcgagg aaaaagaggg
     3121 cgaacagacc gaagatcgat cccgaggtcg ccagcgacat ccgcacgatc tgcgtcagaa
     3181 aaagaaacga cagcggcccc atgacgaccg ccggcggcac gagcagccag gcgacatatt
     3241 tgtgtgtgaa ggtcactgga atcgtgatta tggcagaagc ggctcacgcg aagacgcgaa
     3301 agggatcgca tagaaacctt cgcgtcttcg cgtcttcgcg tgagagggtc actcttcagt
     3361 ctgaaacagc ccgaacgatt cggccgccat cgtcagtacg tttccgttga tgccggcgtc
     3421 tgtctcgatc atcgccctcc acaccgcgtc gtcgaacggc agcccaatca cacgcggcgc
     3481 gtcgctgaag ttgaacgcga gcagcgtgtt gcgccgcttg atgaggagcg tgcgctgctc
     3541 gtcgtcggcg tgggtctcga ccgcgtcgag atcgagctgg cgcagcgcgg gcgtcgtggc
     3601 acgaaggcgg agcaactcct tgtacagcgc gcgaatgtcg tcgagtggct cgcggcggag
     3661 tttcgagcgc aggaacgtgt cgctatcgtg cgggtcgggc ggctcctcgc tccactgaaa
     3721 gcgcgcgaac tctttgtggc ggccgcggcg caccgcttcg atcagatcgc ggtcggcgtg
     3781 cgaggtgaag tactgaaacg gcgcggtctc gccatactcc tcccccatga acagcatcgg
     3841 gatgaagggc gcgaggatga ccgccgccgc cgcaatgcgg actttcgaga gcgaaacctc
     3901 cgccgcgagc cggtcgccgg tcatgcggtt gccgatctga tcgtggtttt gcgaaaacac
     3961 gacgaactgt tttccgtcgc gcgtcttcgg cgccacgccg tgcttgcggc cgcggtaaac
     4021 ggagtgctgg ccggtgtaga ggtaaccgct cgccagcgtg cgggcgagat cgctcgttcg
     4081 tccgtagccg gcgtagtagc ccgaggtctc gccggtgagc agcgtgcgca gcgagtgatg
     4141 aaaatcgtcg ctccactgcg catcgaatcc gaggccgagg tcgtccctcg gcgtgattac
     4201 gcgcggatcg ttcaggttgg actctgcgaa ggtgtaaacg cgccgcccga gcgcgtctcc
     4261 ccgctcgcgc accgccgtcg tcagatcctg cagaaacggc tcggccgtcc gatcggcgat
     4321 ggcgtggacg gcgtcgacgc gcagtccgtc gatgtgaaac tcatcgatcc attgcagcgc
     4381 gttgtggatg aaaaaccagc gcacgtcatc gctgtgcggt ccgtcgaagt tgagcgcatc
     4441 accccacggc gtcttgtagc ggtcggtgaa gtacggaccg tactcggaga ggtagttccc
     4501 ttccggtccg aggtggttgt agacgacgtc gaggaggagg gcgagaccgc gggcgtgaca
     4561 ggcatcgacc agccgtttca agccgcccgc gccaccgtag gaattctgcg cggcgccgat
     4621 atagacaccg tcgtagcccc agttgcgcgt gccggggaac tgaccgatcg gcatcagctc
     4681 cagtgcggtg atgccgaggt ttttcaagtc gtcaagatga tcgatgacgg catcgaacgt
     4741 tccttcgtcg gtgaatgtgc cggtgtgcaa ctcgtagatg acgtaatcct caagtagcag
     4801 gccgcgccac cccgcgtcat gccagtcgaa cgcgctagca acgacctcgg atgcttcatg
     4861 caccccatcc ggctgcgacc gcgacgcagg atcgggacgc tcacggccat tgatgacgaa
     4921 gaagtagcgc gtgcctgggc cggcatcgac gacgacctcg tgatagccgc gcagattcgt
     4981 cgccaactcg atgcggcggt cgtccggcgc aacgatgtgc agctcgactt tctcgacctg
     5041 cggcgcccaa acgcgaaact cgcaacggcc gtcgtcgcgc acgatcgcac cgagcccgca
     5101 tttctcacac acttggtgtg tccctccttg cttcacctct tcctttttct ttgggagtgc
     5161 tttcccttcg agcgccttct ccccccgcgc tttttgcggg gggagaaggt ggcgaagccg
     5221 gatgaggggg gcgtaaatac gcagggcaat caaccatgcc tcgtccgaac tgactgaaaa
     5281 tgcggcatat gcacggatgt cgtacttgtt ggaatgcccc ctcatccggc cttcggccac
     5341 cttctccccc acaaaaaacg tgggggagaa ggcgctcgat tggaaaggtg gcgataaggg
     5401 tgtgagaaaa gcgggctagc ggcggcatgc tcgcggtgaa gctcacgact tgtccagcga
     5461 gttgggccaa tccgagcctc tagccggcga aggcaagcgc taattcggcc gcggcttctt
     5521 cgagatcgcc ctcgccgtcc ggcatcagaa acaccgcgcc gagcggcgga agcgtcaggt
     5581 tcagcgacca gcggcggccg tgcagcggga tcggtgcggc atcgataccg cccatgttcc
     5641 cctgcccgct tcctccataa accttcgcgt cgctgttcag cacctcggac caatgaccgc
     5701 cgatcgggac gccgatcatg tagttgtacc ggggcagcgg cgtgaagttg agtgcgatcg
     5761 ccaccggctg ttccggctgc gacgacgact tccgcaacag gacgaggatg ctgttctccg
     5821 tgtcgcagca gtcgatccat tcgaatccat ccggggaaag atcgagctcg tgcatcgcag
     5881 ggatgtcgcg ataggccttg ttgagatcct cgacccagcg ctgcagcgtc tcattcggaa
     5941 gcgattcgag ctcgttccaa tcgagcgcgg cgtcgtgatt ccattcgcga gactgcccga
     6001 actcccctcc catgaagatc agcttcttgg ccggctgcga gtacatatgc gcgaagagca
     6061 gccgcaggtt ggcgaagcgg cgccattcgt cgcccggcat cttgcggatc agcgagccct
     6121 tgccgtgaac cacctcgtcg tgcgacagcg gcagcaggaa gttctcggcg aacgcgtaca
     6181 tcatccggaa ggtgagatcg ttgtggtgga acttgcggtg caccgaatcg agcgcgaagt
     6241 agcgcagcgt gtcgtgcatc catcccatgt cccacttcat gccgaatccg agtccgccga
     6301 tgtacgtcgg ccgcgacacc atcggccacg acgtcgactc ctcggcaatc acttgaacat
     6361 ccggataggc ctcgtagaca tcctcgttga ggcggcggag aaacgcgatc gcctcgaggt
     6421 tctcgcgtcc gccgtgctca ttcgggatcc attcgccgtg ctttcgcgag tagtcgagat
     6481 agagcatcga ggcgacggca tcgacgcgca atccgtcggc gtggtaaacg ccgagccagt
     6541 agatcgcact cgacagaagg aaggactgca cttctctgcg cccgaggttg aagacgagcg
     6601 agccccagtc gggctggaaa cctttgcgcg gatcggcatg ctcgtaaaga tgcgtgccgt
     6661 cgaagtacga aaggccatgt tcatcggtcg gaaagtgcga cggcacccaa tcgagaatga
     6721 cgccgatgtc gtgctggtgc aggacgtcga tgaggtacat cagatcctga ggcgttccgt
     6781 agcggctcgt cggcgcgaag tagcctgtgc actggtagcc ccaggaaccg tagaagggat
     6841 gctccatcac cggcagcagc tcgacgtgcg tgaagttcat cgtgcgcagg taatcggcga
     6901 gcggtcgggc cagctcgcga tacgtgagcg gacgctcgac gccgtcgtcg cccggcgtgc
     6961 gccgccacga accgatgtgc atttcgtaga tcgacattgg tgcggaaaac gcgttgcgcg
     7021 cagcacgctg ctccatccag gcgccgtcgt tccattcgta gtcgaggtcc cagacgaccg
     7081 aggccgttcg cggcggcatc tcgtgcatca cgccaaacgg atcggccttg tcgacgcggt
     7141 agttgttgaa gcgcgagcgg atgtgaaact tataatgcac accctttccg acgccgggca
     7201 cgaagccttc ccagatgccg gaaggaccgc ggttttccag cggattggca tcaggacccc
     7261 acccgttcca gtcgccgatc acgctgaccg cgtccgcatt cggcgcccag acggcgaaca
     7321 tcgttcccgg cacgccgtcg cgcgtgtgcg gatgcgcgcc gagtttttcc cacagccgct
     7381 gatggctgcc ctcattgaag aggtgatagt cccaatcact caaaaggctg tcacccggct
     7441 ttgcgctgtc tgttgccgtc acttcgacgt gttccttccg aggagagtcc acctcgatcg
     7501 tcacgtgcgg cacgacgtcc gcgccgaccg cctgcgcgac ttcggtcacc cgattgacct
     7561 tcggcgcgcg tttgcgggga gcggccttcg ccttcttcgg cgcatcagct ttggtttttg
     7621 tagctcgctt gcgcggcgac gccttctttt tcgatggcgc atccgcttcg ggcgtcacat
     7681 ccgtgatcgg atcagttcgc tcggcaggag catcgccctc cgtttcttta ccgtccatca
     7741 accttccttt ggaaccgctt cctagcaatg tgcgagccgc tgggtcaggt ctaaagtcta
     7801 aagtgccgat ttggctgacc gcctgagcga caatcgtcct cggcacttta gactttagac
     7861 ctgacccatt catggccgac tttatgctac gtccctcccg ggatggtgcc aatggcgaac
     7921 gggaacgttc tagtcgtcga ggatgacgaa gccgttcgaa ggctgctcat cgattatttg
     7981 cacctgaacc acgccatcga cgtcgacggc gcgcgcgacg gcatcgaggc gttgcaccag
     8041 atcgtcacca accgctactc ggtgatgatt ctcgacttga tgatgccgaa gatgagcggc
     8101 gtcgatctgc tcgactccct cgaagcgctc atctccgacc cctcggtcaa agcactcgat
     8161 cacctgcccg cggttttcgt catcacctca tttgccaccg ccgcactccc cgacgacgtc
     8221 atccaccagc gctgccccca agccgtccag ggcatcttcc gaaagccgct cgacatcgag
     8281 aaggtgtcgg cgagtgtggg gaggtatctg gatgcgtaga gcgcgggagt agggggtcgg
     8341 gggtcggggg tcggagcggg gctcacgaat taatcgaagt ctctcgacaa acgaagaggt
     8401 gacacgcggg gatcggctcc gactcccgac ccccgacaac actacgaccc ccgcaacagg
     8461 acgacccccg caaaactcac cacacctgac acgctttctt gttcaccatc ggcgcatcgg
     8521 cgggacagtt gaacgctttc tgaaactgcg gcagatccga gacgatgccg ttgatccgat
     8581 acttgccggg tgaatgcgga ttcgtgatcg cgttcgcgcg cagattctcc ggacgctggt
     8641 tctcgcacgc ccattgcgcg aagccgatga agaagcgctg atcgggcgtg aagccgtcga
     8701 tcggctgcgt gtcattcgga tccgctgcct tccacgcgat gtaggccagc agcgttccgc
     8761 cgaggtcggc aacgtcctcg ccggaggtga gcttggagtt gatcttgatg tcgtcgacga
     8821 tcgtgtagcc ggcgtactgg tcgcgaacgc aattcacgcg gtcttcgaac ttcttcgcgt
     8881 catcgggcgt ccaccaatcc ttcaggtttc ccttcgcgtc gaactggcgg ccttcgtcgt
     8941 cgaacgcgtg cgtcagctcg tggccgatcg tcgagccggt gttgccgtag ttcggcgcgt
     9001 cgtcgagctt cgcgtcgtac agcggcggct gcagcacgcc ggcggggaag ttgatgtcgt
     9061 tcatctgcgg gttgtagtac gcgttcacgg tcggcggcgt catgccccac tcgttgcggt
     9121 cgaccggctt gccgatcttg gccagatcgc gctgcgactc gaacaacgtg gcccgcgtcg
     9181 tgttgccgta gaaatcgccg cgaacgactt tcagcgtgct gtagtcgcgc cagcggtcgg
     9241 gatagccgac cttgttcgca accgcgtgca acttctccag cgcccgctcc ttcgtcgccg
     9301 gcgtcatcca gtcgaggttc ttgatctcgt cttccatcgc tgtctcgatg cgcttcgtca
     9361 tatcgacggt cttggccttc gtctccggcg tgaacgtccg ggccacgaac tcctggccga
     9421 gcgcttcgcc gaggtcgcgg tcgacgagct ggacgcaacg cttccagcgc ggcggcatcg
     9481 acttcacgcc gcgcagcgtc ttccggtaga agttgaaatc ctcttccagg aacttcttcg
     9541 agagatacgg cgcgcgggca tgaacggcgt gccagcgcag atacgccttc caatccgcca
     9601 ggggaacgga cttgatctcc ttctcgacgg ccgcgaaaaa cttcggctcg gtgacgttca
     9661 gcgtttgcac gttgctgatc acgccgcttt cgacgagata ggacttccat tcgaacgccg
     9721 gcgcgatctt gctgaagtcc gctaccgtca tcttgtgggc caggttgtac ggattgcgct
     9781 tctcgacacg cgtcaacgag gccgtcgcca acaccgtctc gatgtgcatc accgtcgccg
     9841 catccttctt cgcctcggct ttcgactccc cgatcagctc gagcatcgca gcgacgtgtg
     9901 caacatactt ctcgcggatc tccttcgatt tggcgtcggt cttggtgtag tagtcgcgat
     9961 ccggcagtcc caaccctcca gcgctggcct cggcaatcac actcgacgca tcgccgtagt
    10021 cctgcgtcga tccgaagccg aagatccagc cgttgccgtt cgagcgcata tgctgacgcg
    10081 caagaaagcc tcccagctcg tacttcgcct tcagtcccgc gatctcgtcg agcagcggct
    10141 gcaacggctt cgcgccgagc tcctcgacgt gcgcctcatc cgtgcaggca tcgaagaaat
    10201 cgccgatctt ctgctgcacc gccgtccggt ccgcgcgcgg cttggcggca tcgagcagga
    10261 tcccccagag gtactcctcg ttctcctcgg tgaccttgcc gtagacgctc cacgacgcct
    10321 gatcgcccgg gatgggattg ttcttcatcc atccgccgca ggtgtattga tagaaatcgg
    10381 cgcacggatt cgctgtccgg tccatcgacg gaatgtcgag gctcggcgtg tacggcaacg
    10441 ccgaaagcgg atgttccttc ggcgccggcg cggcaggcac cggcgattcc ttcggcggcg
    10501 gcgccgcatc ggacggcttg gcggccgaag gcttcggcgt ctccgccgga cgcgcattct
    10561 tcaccggatc aggattctgt tgcgcgaaca aactggtggc tgcgaggagg atgacggctg
    10621 cggcgatctt catggccggg aagtctagcc cggatgctcc aggttcggag atggtgtctt
    10681 tcgaaccagg gagggtgggg ttcgcacccc cgattctgcg ggtcgcaccc acgatgctgg
    10741 gggttgcacg cacgatgact tgttccgcac ccacgatgct gggggttgca ctcacgacga
    10801 catgttctgc acccgcgatg ctggggtttg cactcacgat gacttgttcc gcacccacga
    10861 tgctgggggt tgcactcacg atgatgtgtt ctgcaccccc gctgccgtgc ttctcacccc
    10921 cgattctgca caactcaccc ccgatgctgc acaacttccc cacgatgctg cacgactacc
    10981 ccccgatgct gctcaactac ccccccgatg ctgcgcaact tccccccgat gctgcgcaac
    11041 tcactcccga tgctgcgcaa cttccccacg atgctgcgca acttccccac gatgctgcgc
    11101 aactcactcc cgaggctgtg tttcgaacca tcgacggtgc cttttgatcc atcgtcggtg
    11161 cttcttctta gttccgctct ggcaacccct tgtcaccgca gagacgcaga ggccgcagag
    11221 cgtagagctt cagctctgtg tcctcggtgt ctctgtggtt cattcttgtc caacctcccg
    11281 gaggagttcg ggcacctccg gcagctcagg tacaatccgc gttttcccaa ccaggagggc
    11341 acccatggcc gatctgtcga cgacgttcac cggaatccga ttcgagaatc cctttttgct
    11401 ggcatccgcg ccaccgacgg agtccgacgc gaacatcatc cgtgccttcg aggccggctg
    11461 gggcggcgtc gtcaccaaga cgatcggcct ccatcccgtc gtcaacgtca aaggaccgaa
    11521 gacgaagttc ctccggtccg aggatcaggg gacgcgtatc tcgatgacga aacggcccgg
    11581 cacgaccgtg atggcgtcgt ggaactggga gctgatctcc gacaagccgc tggaatggtg
    11641 gctgggcaag cttgccgcca tcaagaaggc gtatccaaaa cacatcatcg tcggctcgat
    11701 catggccggc tccggcaacg acaaagagct gggcaattgg cagacgctgg ccagggcgct
    11761 gcaggacgaa ggcgccgacg cgctggagct gaatttctcc tgcccgcaca tggaccgcgt
    11821 cgacatgggc tcgaacgtcg gtaaggacaa ggttctctgc tcggtcgtca cgcaggccgt
    11881 gaaacaggtc tcgaagatcc cggtgtgggt gaagctgacg ccggccaccg cgaacatcgt
    11941 cgaagaagcc gaagcctgct tccgcggcgg cgccgacgcc gtcgtctcat cgaacacgtt
    12001 cccgtcgctc ccggtgatcg atcccgccac gctcgagttc gagatgaacg tcgacggcta
    12061 tgtctcctcc ggcggcctcg gcgggcccgc aatcctcccg ctgtccctcg ccaagatgtc
    12121 gcaactgacg agagagttcc ccgacaaaga gttctccggc atcggcggca tcgccaactt
    12181 cgaccacgcc ctcaactact tcctcctcgg ctgcggcacc acccaggtct gcaccgccgc
    12241 catgctcgac cacgccatcg gcccgaacgt gatcaaagcg ctcaccgaag gcat
//
LOCUS       DATLVW010000189         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_176303, whole genome shotgun sequence.
ACCESSION   DATLVW010000189 DATLVW010000000
VERSION     DATLVW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176303"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VNN25_06065"
     CDS             complement(<1..446)
                     /locus_tag="VNN25_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91126.1"
                     /translation="MALLATTAFAQPANTITINDDQPVYAEGVPFMLSGTSAPGAVIA
                     VAIEKSGNGQTTADTTGHWTLMWTPPVKTGTWTITASSGGVTATQLLRVQLPGNVQRQ
                     PIVEPVLRYGTPEIPHPEAYQEMTDRWRIAPPPYELTEKPKARAIG"
     gene            complement(554..>2261)
                     /locus_tag="VNN25_06070"
     CDS             complement(554..>2261)
                     /locus_tag="VNN25_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXH91127.1"
                     /translation="CVIPLSASVPDVCTDGTIGNGTPESFNAISKAEANAIVQAAATA
                     LAGDTATIAIVDRTGRPVAVFRQANANPANDDIALGVARTAAFFSNSQAPLSSRTVRT
                     ISQVHFPPGVVNAPVGALYGIEQSNRGCDVNLWWNANQCVPRAKSIGQFSSFTGAPKP
                     CPAADRSGCGPGLVTGKTLPDDGPYPQEVGNRPVNAGGIPLYRILDVSAVKQGIARTG
                     KLLGAIGVTGIPGDPQLGEFAAVTGAFGPLNAGIAPIIPLPNYPLPDPQNVFIDGIRL
                     PFIGPDQKLRFNTDGLPVGLELLKGASAGTAAGFTGGTFVYGPFNGGCPPVGYLVGPN
                     ASALPGGLTATQVDAIIQRSVAASKRTRAQVRLPLSSYTRMIITVADIDGTILAHYRM
                     PDALFDAVDVVLAKARNVAYFNGSDPHARNDLPGVPAGYWVTGRTIGFGAQPLYPPGI
                     DSNVFEVGPGPWYSTLFLPNIANTCSQGSQAPNPNQNGVTFFGGSTPLVQNGKLVGGL
                     GVSGDGIEQDDYVTYLGAGDLLPPTDKWADQLKVNGARLPMFKFPRSPEGVTECGGKP
                     CS"
BASE COUNT          389 a          671 c          793 g          408 t
ORIGIN      
        1 ttgccaatgg cgcgggcctt tggcttttcg gtgagctcgt acggcggcgg cgcgatcctc
       61 cagcgatccg tcatctcctg atacgcctcg ggatggggaa tctccggcgt gccgtagcgc
      121 agcaccggct cgacgatcgg ctgccgctgc acgttgcccg gaagctgcac gcggaggagt
      181 tgcgtcgcgg tgacgccgcc ggagctggcc gtgatcgtcc acgtgccggt cttcaccgga
      241 ggcgtccaca tcaacgtcca atgtcccgtc gtgtcggcgg tggtttgccc gttgccggat
      301 ttctcgattg cgacggcgat gacggcgccc ggcgccgacg ttccgctcag catgaacggc
      361 acaccctcgg cgtagacggg ttggtcgtcg ttgatggtga tggtgtttgc gggttgcgcg
      421 aaagcggttg tggcgagtag cgccaaagca acgaagaaaa acgaagcgaa acgtaggtcg
      481 gactctctag tccgaccaat cgcgccacgg agtcggactg gagagtccga cctacgtttg
      541 cttcgcgagg cgtttacgag cacggtttgc ctccgcactc ggtgacgccc tcggggcttc
      601 ggggaaactt gaacatgggg aggcgtgcgc cgttgacctt gagttgatcg gcccacttgt
      661 cggttggtgg aaggaggtcg ccggcgccga ggtaggtgac gtagtcgtcc tgctcgatgc
      721 cgtcgccgct gacgccgagt cctccgacga gcttgccgtt ttgcacgagc ggcgtcgagc
      781 cgccgaagaa cgtcacgccg ttctgattcg ggttgggcgc ctgcgatccc tggctacacg
      841 tgtttgcgat gttcggcaga aacaacgttg agtaccacgg ccccggaccc acctcaaaca
      901 cgttcgagtc gatgcccggc gggtagagcg gctgcgcacc gaatccgatc gttcggccag
      961 ttacccagta acccgcggga actccgggaa gatcattgcg ggcatgcgga tcgctgccgt
     1021 tgaagtatgc gacgttgcgc gctttcgcca ggacgacgtc gaccgcatca aacagcgcat
     1081 ccggcatccg gtagtgcgcc aggatcgttc cgtcgatatc ggccacggtg atgatcatgc
     1141 gggtgtagct gctgagcggg agccgcacct gagcgcgcgt ccgtttcgac gcagcaactg
     1201 agcgctggat gatggcgtcg acctgagtcg cagtgagacc gcccggaagc gcgctggcgt
     1261 tcgggccaac gagatagccg actggaggac agccgccgtt gaacggtcca tagacgaagg
     1321 tgccgccggt gaagcctgcg gcagtccccg ccgatgcgcc cttgagcagt tcaagtccga
     1381 ccggcaggcc atcggtgttg aagcgcagtt tctgatccgg cccgatgaaa ggtagacgga
     1441 tgccgtcgat gaagacgttc tgcggatcag gaagcggata gttcgggagc gggatgatcg
     1501 gcgcgatgcc ggcgttgaga ggcccgaatg cgccggtgac cgcggcaaac tcgccaagct
     1561 gcggatcacc gggaatgccg gtgacgccga tggcgccgag cagtttgccg gttcgggcga
     1621 tgccttgctt gaccgcgctg acatcgagga tgcggtagag cggaatgccg ccggcgttga
     1681 ccggccggtt gccgacttcc tgcggatacg gcccatcatc gggcagagtc tttccagtga
     1741 ccagaccggg gccgcagccg gagcgatcag cggccgggca gggcttcggt gcaccggtga
     1801 aagaggagaa ctgtccgatc gacttcgcgc gcggcacgca ctggttcgcg ttccaccaca
     1861 gattcacatc gcatcctcgg ttggattgct cgatcccata caaggcgccg accggagcgt
     1921 tgacgacgcc cggcgggaag tgaacctgag agatcgtgcg cacggtgcgc gacgagagcg
     1981 gcgcctgact gttgctgaag aatgcggcgg tgcgagcgac gccgagggcg atgtcgtcgt
     2041 tagctgggtt ggcgttggcc tggcggaaca ccgcaaccgg acggccggtg cggtcgacga
     2101 tggcaatcgt ggcggtatcg cccgcgagcg cggtcgccgc cgcctgcacg atcgcattcg
     2161 cttcggcctt cgagatggcg ttgaacgatt cgggggtgcc attgccgatc gtcccgtcgg
     2221 tgcagacatc gggaacggag gcgctcagag gaatgacgca c
//
LOCUS       DATLVW010000190        14044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_503612, whole genome shotgun sequence.
ACCESSION   DATLVW010000190 DATLVW010000000
VERSION     DATLVW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503612"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..694)
                     /locus_tag="VNN25_06075"
     CDS             complement(59..694)
                     /locus_tag="VNN25_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91128.1"
                     /translation="MRKTKKSVANPSNPVTAAPSADDVAAQLMGLIDQMEALVPGFTP
                     HDTNDARRVANVARFAKDLIPQAITTVTALPQIAGINIFDVEEGKAALAFDASMQPFV
                     QRLASLLDGVSFTIGKRLATSTGQGLAMFAWLKKHAKTPEGVAVRPYADEMSRTVKKT
                     LNRRKPVHPPTPPPAGAQTLLAPNLAQAAATATDDDLPEDFRKELEEAVKD"
     gene            complement(1232..1873)
                     /locus_tag="VNN25_06080"
     CDS             complement(1232..1873)
                     /locus_tag="VNN25_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91129.1"
                     /translation="MSNQREIAATTTEAADAPPADGSMPDIAAVMALIGQIEAQIPGF
                     TPHDSNHAKRVANMTRFAKDLIPQMIATVSSLPSVVGMSTFDVAAGKTSLAFDTAMQP
                     VIQALSALLDGAQFTTKSRLARSTGQALATFAWMKKTAKGPDGVALRPHVDDMALTMK
                     KALNRRSKPASKPPVQEAAQDSSAPNPDQGNATVSDDDLPDDSPAALEKTVKE"
     gene            complement(2364..2882)
                     /locus_tag="VNN25_06085"
     CDS             complement(2364..2882)
                     /locus_tag="VNN25_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91130.1"
                     /translation="MSATISYGPRRLPVVMAMEVGMIAQAVARVSGISPRPRLEGALW
                     RASPAMATLTPTPKPVDMRATPATKERGIDDAGAKDLLRAAIDLLRAAKSLLLAAKDL
                     LAAANDLLVVANDFLVVANNLLAVANNLLAVANELLAVANELLAVANDFLAVANELLG
                     AANDLLGASKDH"
     gene            2830..5442
                     /locus_tag="VNN25_06090"
     CDS             2830..5442
                     /locus_tag="VNN25_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH91131.1"
                     /translation="MAITTGSRLGPYEMVALIGAGGMGEVYKARDTRLDRSVAIKVLP
                     SAFAGNAQLKLRFEREAKTISQLTHPHICTLYDVGNDQGVEYLVMELLDGETLADRIA
                     RGPLPMRDVLRYGGEIAEALDRAHRAGVVHRDLKPGNVMITKSGAKLLDFGLAKGGAL
                     EITADGATQQKSLTQEGTILGTFQYMAPEQLEGTEVDARTDIFALGALLYEMATGRRA
                     FEGKTKTSLIASIVGGEPKPIREIQPLTPPAFEHVVAKCLAKDPDHRWQSASDVASEM
                     EWIGTSSSQSQSVSDVARRRGRYRGMVIALIALAVIAAAAASVFLLRRPAAAETPLMM
                     SITLPKHAGIDAFGQAALSPDGSSIVFIARAVDSSSSGVTTMSLWVRPLDRAEPRLLP
                     GTEGATEPFFSPDGRSVGFFAQGKLKRIAIAGGPPQTICDAPLAFGAAWSRDGVILFC
                     GLHGPLFRVNANGGTPRPVTRLSPGEEAHRWPVFLPDGDHFVFLGDAPRTENHHLKVG
                     TLHDGSSRDLVQAVTNVNYVEPGYLLFVRAGSLMAQPFDPKTLSLAGEPRVLAEQVVT
                     NDDNHHFEFSASQNGRLVYRSGSPDSQLIWVDRSGHELATVGDARRLGWEARLSPDQQ
                     RIATDRNDADGRSDDIWILDFARNVTTRFTFDPAGDYSPVWSPDGSTIAFASMRAGLG
                     NLFLADAANPTNVRRLTNFPTDQIRPVSWTRDGKMVVAERADKAGIDLWLFPVDGSEG
                     KPYLATPFVELSADVSPDGTYIAYASDESGRDEVYVERFPSHAGRRQVSSSGGDQPRW
                     RNDGRELFYISHGEVVGVDMTSNTATPKPLFRLPGNSYDIARDGQRFIVDRPLDDNFS
                     SPLTFVSNWMAERK"
     gene            5669..11440
                     /locus_tag="VNN25_06095"
     CDS             5669..11440
                     /locus_tag="VNN25_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXH91132.1"
                     /translation="MKRAVLTIVLFLTAVSAFAADKNLTIIKAGPIGEVANLAEANEI
                     RVVFSEPMVVVGKIPKALEVPWFHVEPAINGSFRWSGTTTLIFTPDPKTPLPFATKYD
                     VTVDAAAKAVSGKTLGKAYHFSFTTPTIQLLRTDWYRKGGKYDGAIVIALWFNQPVDG
                     ATLGSHLVLRTVAHEFKEPTLPARDRLAKLEPAQPAAFDAKIAKAQQAAASDGQPVLS
                     FLATEWDKKHFKPVPEMVIVETKPGVPPDTNLQIFVDDKLAKNANSVASGHPQTFTIE
                     LGPTFFIEKADCLAECGPDYYNPISFRVNGGVPYVNFKKAVSVIDVTDPAHELPVTPS
                     EPKRAVDPSAAPATQYSLDELGYSVIPAHTYAVRVDPSLKSENGQTLGYTWMAVIEYW
                     HKAAYISFGDGHGVWESSGGPMLPFHARNFKNVTQWMTPLSLDKLVPTIVDLQKEGFH
                     KTPATGGAQRNLAPVADKLQSYGLNLKSALSSIGNGIVWAVIKPGDAIAKSKTYSEDT
                     AGTIVQVTNLGITVKDSPQNTLVFVTRLDNAAPVAGAKVSIRTIENKVFWTGTTDANG
                     IAIAPDTDLRVERNKKNEKGEEVPVESWEAFGLHFVVTAEKDGDTAYASSDWNDGITP
                     WEFGLGFDLDEAKPLLRGTTFTDRGVYKPGEEVHAKIVLRSDTPKGMQLLPAGTEVDI
                     VVTDAHDKEVDKRTLKLNDWSSAEWVWTVPADGVLGDYGIKASVKSQRLMTFGSFLVA
                     AYRRPEFRVDVTLKGAPPIAGTKLNGTIIGKYLFGGAMASRPVKWTYSKVPVYDVPSA
                     IRERFQEGQWEFLGEDPEQRREGETINTQEPKLDAKGELKLTLDTDIAAGVPWSYVLE
                     GNVTDVSRQQLANRTAFRVHPAPWYIGVKSPNYFSDATKGLDTAIVAAALDGTATAGV
                     KVSVDLHRIQWNSVREAAGNGFYNWSTERKELPAGHWDVTTQATPAPLHIPLGEGGEY
                     LIIATAKDAEGRSTTTRTWFYAVGGGYTAWARADSNRIELVPEKKTYKPGDTARIMVK
                     SPWEHATALLTTEREGVRTWKTFELTSTQTTVSVPITEKDIPNLFVAVTLLKGRTKDG
                     IEDESDPGKPAFRLGYTALSVVDAAKRLKVAVKANRDEYRPASKARIEVDVHDVAGKP
                     SQSEVTLWAVDYGVLSLTAYETPDVLQSIYLEKSLQVLNFDSRQKIVSRRVLTPKGET
                     QGGGGGKDAGPGVMRKDFRVLAFWVGSVVTDAKGHAKTDVTLPESLTTYRIMAVAGDR
                     GSRFGWAQNEIRINKPVLLTPTWPRFLAVGDKAFFGGVVHSQLKQPGKATVTIKSLDP
                     SIVEFTQPTSQTVDIAAEGSAEVRFNASAKAVGNARLQMTVSLNKETDAFEDSLPVRI
                     LTPSETVAAYGEAKPDAKETLAVPKDVVPSSGGLHVELSSTAMVGLEEGASYLVDYPY
                     GCAEQRSSGAMALMLSSDLGEAFALPGIDPKKGRTIAQATINELNNFQCGDGGFAFWA
                     GECYTESPYMTATVLHVMQRGEKLKYDVNKDVTKRGYDYLDAKLKEPRPTNEGWWPAY
                     TAWQAFAVKVLTEGGRNDDSHITRLYSYVDRMPLFGISYLADAMLAKGEKGARVEDLT
                     RRITNGILPEGGSAHVEELSDPYLLYYWSSSVRSTAIALGTLTRHGESEQMVKQMVRW
                     LMKVRRQGRWGNTQENVWAMESLVDYYRKYESETPDFTAVVKIGADTIANDSFKGRSA
                     EAKNHDVPMQQLMARPPGDTPVTFDRQGTGTLFYLMRLRYTPAGLMHDALNQGFSVER
                     KYSLKTFKAGDLIKVTLRIRNTKERRFVAVIDPIPAGTEPVESWFATTASDLAAEQSK
                     NDTQGDDWMSWWKHGGFDHVERHDDRVNLFATRLGEGMHEFTYMVRATTAGTFITAPT
                     HAEEMYEPEVFGRTASEVIEVKP"
     gene            11446..13656
                     /gene="pbpC"
                     /locus_tag="VNN25_06100"
     CDS             11446..13656
                     /gene="pbpC"
                     /locus_tag="VNN25_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HXH91133.1"
                     /translation="MKVERRRLAGWTAGGLAGGLLALALWVRLGPIPTITPDTSPVIV
                     DRNGVVLYEPLSPSGTRNEWINAVPESIAAATIAAEDRRFEHHPGIDPVAIARAVAHD
                     IRHLRAVEGGSTITQQVAKMLLGNPPRTIVSKLREAVIALRLEHRYTKRQILALYVNL
                     APYGEQTIGVARASRRYFGCAPEELTTAQSAWLASLPQRPSTPRRALARQRVVLARMH
                     SLGLINDASWREARAERLTLDRGIQPTLAPHFVEQVRSRSGAQPNHLVKTTLDAVLQQ
                     NVRGIIAAERANLLRHGAHSVAVVVLDNETGGWLAWEGSGDYFGTDFGGAIDGVTTLR
                     QPGSTLKPFTYALAFEEGITPATVLADVPSHFPTAEEGVVYTPRNYDGGYRGPLRARA
                     ALAGSENVPAVALLEKVGPPALLRMLRGTGFRDLTHTADYYGLGLTLGDAEVTLEQLV
                     SAYATFARGGTSIATTMLLRPPAPDSRPPRIVSPRTAFWITDILSDSHAREFIFGRGG
                     NLDFPFPVAVKTGTSQAYHDNWTVGYTRAVTVGVWVGNFDRQALRSSSGVTGAGPIFH
                     SVMLAAMRRAGDNGEREIVDRPDDLEQQPICALSGLRPSTSCPAIESEWLPRDAPVEF
                     CAWHHAGRIDWPEEYRDWASARERRTPASAPRALAGRETRFRITSPSNDTTYLVDPTL
                     HREFQAIHLRATSDATWSVDGRAVSSEWPLRTGKHSIVATNRRGERDAVAITVR"
     gene            complement(13807..>14044)
                     /locus_tag="VNN25_06105"
     CDS             complement(13807..>14044)
                     /locus_tag="VNN25_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91134.1"
                     /translation="IKVHPVRTGAQNGRHDESTIPCVVTQNGRVHLAHGHTPLAITFQ
                     HASQFTNIAAHIPQDNVAATGDHEVDLISRLDAQ"
BASE COUNT         2699 a         4357 c         4476 g         2512 t
ORIGIN      
        1 gtgaccgatc accaaaacac cgccccttgc gcgaggtgcg cgaggggcgg cgtcgggtct
       61 aatccttcac ggcctcttcg agctccttgc ggaaatcttc cgggaggtcg tcgtcggtgg
      121 ctgttgcggc ggcttgggcg agattcgggg cgagaagggt ttgcgcgcct gccggtggcg
      181 gcgtcggggg atggactggt ttacggcggt tcaaggtctt cttgaccgtt cgcgacattt
      241 catccgcata cggacgcact gccaccccct cgggggtttt cgcgtgtttt ttcaaccagg
      301 caaacatcgc caatccctgt ccggtggatg tcgcgaggcg tttgccgatc gtgaatgaaa
      361 cgccatcgag cagggatgcc agacgctgca cgaagggttg catagaggca tcgaatgcca
      421 gagccgcttt cccctcctcg acgtcgaaga tgtttatgcc agcgatttga ggtagcgcgg
      481 tcacggtggt gatggcctgt gggatgagat ccttggcaaa gcgcgccacg ttggctacgc
      541 gcctggcgtc attcgtatcg tgcggcgtga agccggggac gagagcttcc atctgatcga
      601 tcaggcccat gagctgggcg gcgacatcat cggccgatgg tgcggcggtg actgggttgg
      661 acgggttggc aacactcttt ttcgtttttc gcatgatcgt tcctcgaaac cacgcgggac
      721 agactctctc tgtataccgc gtgggttagc ggtttagtca attcgagcca gctctgtacg
      781 ccggcagcga cgcgctgacg gcgaggttca tcgatgagac ctgtttcctt agacgagctc
      841 ggtcgagcaa ggttacatag ggttccggag cgacaagttt ttcaggttga ttgagcgagt
      901 ttcctcgctg cgcaacgaat gatgcggtca cgcgttagtg tcatcctgag ccgccgcagc
      961 cgccgcaaga ttcgcgacac ttgcaaccgg cagacatcgg cggcgaagga cggtcgaagg
     1021 atctcaaaat gcgacggtct agttcgggcg tcattgatca cgatcgctcg cgctggacga
     1081 acgcgatctg cgaagtttga gatccttcga cgtcctccgc cgccgagcac atactgcgca
     1141 aacctcgcga ttcgcgtggg cggctccggc agtctcagga tgacactaag ctcggttgga
     1201 cgatcactca ggatgacctc aacgctaagt gttactcctt caccgtcttt tcgagcgccg
     1261 cgggggaatc gtccggcagg tcgtcgtcgc tgaccgttgc attgccttgg tcgggattcg
     1321 gcgcggaaga gtcttgcgca gcttcctgta ctggaggctt cgatgccggt ttgctgcggc
     1381 ggttcaaggc cttcttcatc gtaagggcca tgtcatcgac atgcggacgg agcgcgacgc
     1441 catcgggacc tttggcggtt ttcttcatcc aggcgaaggt agccaatgcc tgtcccgtgg
     1501 atctggcaag acggctcttg gtagtgaatt gagcgccgtc gagcaacgcc gacagggcct
     1561 ggatgaccgg ctgcatggcg gtgtcgaacg ccagtgaggt cttccccgcg gcgacgtcga
     1621 acgtgctcat tccaacgacc gagggaagag aagagaccgt ggcgatcatc tgcgggatca
     1681 ggtcttttgc aaagcgggtc atattggcga cgcgtttggc gtggttgctg tcgtggggag
     1741 tgaacccggg gatctgcgct tcgatctggc cgatcagggc catcacggca gcgatatccg
     1801 gcatggatcc atcggcaggt ggtgcatcgg cagcttcggt tgtggtggcg gcgatctctc
     1861 tctggttgct cataagatcc tcgaagaatc acgctggtcc ggcttgccgt gacccgcgtg
     1921 ggtgaatggg ttgaggtact tcaagccatc tcgacatctc gtcagcgaca cgctggcgag
     1981 cgaggtcatc catcgaccta ttgattgaga cgaaactgcg tggcagaaag ttacatcggc
     2041 gtaagagagg gtcgtgagcg cgcggggtag tcgccgcttc tggtgattta gggtgctctt
     2101 gggaccgatt cgtccagtct ttgggaatcg gatcgtcgag tgttctagct gcattgtcga
     2161 ttcgacgatg cggagcagtg aatcgacgat ccacatcaac aatctgacga tgcagagcga
     2221 tccatcgacg ctgtgcatcg tcgatccgac aatgtggagc gatcaaatgg caattcacat
     2281 gattgatgtg accatgccga tcgtggactc gacgatgcgc ccagtcgatc tgacgatacg
     2341 cagcctacga ttgacgatgt gcatcaatga tccttcgatg cgcctagaag atcgttcgca
     2401 gcgcctagaa gttcgttcgc aacggctaga aagtcgttcg caacggctag aagctcgttc
     2461 gcaacggcta gaagctcatt cgcaacggct agaaggttgt tcgcaacggc tagaaggttg
     2521 ttcgcaacga ctagaaagtc gttcgcaacg actagaaggt cgttcgcagc ggctagaaga
     2581 tccttcgcag ccagtagaag actcttcgca gcacgtagaa gatcaatcgc agcgcgcaga
     2641 agatccttcg cccccgcatc gtcgattcct cgctcctttg ttgctggcgt tgccctcata
     2701 tcgaccggct tgggagtagg ggttagcgtc gccatcgccg gcgaagcccg ccaaagggcg
     2761 ccttcgagcc gtggtcgtgg cgagatcccc gagacccggg caaccgcctg tgcgatcatc
     2821 cccacttcca tggccatcac cactggcagc cgcctcggtc cgtacgagat ggtcgcgctc
     2881 attggcgcgg gcgggatggg tgaagtgtac aaagcgcgcg acacgcggct ggaccgcagt
     2941 gtggcgatca aagttctccc gtcggcgttt gccggcaacg cgcagctcaa actccgcttt
     3001 gagcgcgaag cgaagacgat ctcgcagctc acgcatccgc acatctgcac gctctacgac
     3061 gtcgggaacg accagggtgt ggagtacctg gtcatggagc ttctcgatgg cgagacgctc
     3121 gcggaccgca tcgctcgcgg accgttgccg atgcgcgacg tgctgcggta cggcggcgag
     3181 atcgccgagg cgctggacag agcacatcgt gccggggtgg tgcaccgcga tctcaagccg
     3241 ggcaacgtca tgatcaccaa atcgggcgcc aagctgctcg acttcgggct ggccaaaggc
     3301 ggggcgctcg agatcacggc cgatggtgcg acgcagcaga agtcgctcac gcaggaaggg
     3361 acgattctcg gaacgtttca gtacatggcg cccgagcagc tcgaaggaac ggaagtcgac
     3421 gcgcgcacgg acatcttcgc cctcggcgcg ctgctctacg agatggccac cggccgccgc
     3481 gcattcgagg ggaagacaaa gacgagcctg atcgcgtcga tcgtcggcgg cgagccgaag
     3541 ccgatccgcg agattcagcc gctgacccca cccgcgttcg agcacgtcgt cgccaaatgc
     3601 ctggccaaag atccggatca tcgctggcaa agtgcctcgg acgtggcctc ggaaatggag
     3661 tggatcggga cttcgagctc acagtcgcag agcgtcagcg acgtcgcgcg aaggagaggg
     3721 cgttaccgcg ggatggtcat cgcgctcatc gcactggccg tgatcgctgc ggccgctgcc
     3781 tcggtgttcc tgcttcggcg cccggcagcc gccgagacgc cgttgatgat gtcgatcacc
     3841 ttgccgaagc acgctggcat cgacgccttc ggtcaggccg ccttgtctcc cgatgggtcg
     3901 tccatcgtct tcattgcgcg tgccgtcgat tcctcgtcat caggcgttac gacaatgtct
     3961 ctctgggtac gaccactcga tcgggccgag ccgcgtctgc tgcctggtac agagggcgcc
     4021 accgagccgt tcttttcgcc cgacggaagg tcggtagggt ttttcgcgca gggaaagctg
     4081 aagcgcatcg ccatcgccgg gggaccgccg caaacgatct gcgacgcacc gttggcattc
     4141 ggggcagcgt ggagccggga cggcgtcatc ctcttctgcg gcctgcacgg gccgctcttt
     4201 cgcgtcaatg cgaacggtgg gacaccgcgg ccggtgacgc ggctcagccc tggtgaagag
     4261 gcgcatcgct ggccggtgtt tctccccgac ggcgatcatt tcgtcttcct cggtgatgct
     4321 ccgcggaccg agaatcatca tctcaaagtc ggaacgttgc atgacgggag cagccgcgat
     4381 ctcgtgcaag ccgtcacgaa cgtcaactat gtcgagccgg gataccttct cttcgtgagg
     4441 gcagggtcac tgatggcaca gccgttcgat ccgaagacgc tctcactggc cggcgaaccc
     4501 cgcgtgctcg ccgagcaggt ggtcacgaac gatgacaacc accacttcga gttcagcgcg
     4561 tcccagaacg gacggctcgt ttaccggtcg ggcagccctg actcgcagct catctgggtc
     4621 gaccggtccg ggcacgaatt ggctactgtc ggcgacgcca ggcggctggg atgggaggcg
     4681 cgcctctctc ccgatcagca gcggatcgct acggaccgga acgacgctga cggccgctcc
     4741 gacgacatct ggattctcga tttcgcgagg aacgtgacca cgcgcttcac gttcgatcct
     4801 gctggcgact acagcccggt gtggtcgccc gacggatcga caatcgcgtt cgcttcgatg
     4861 cgcgcggggc ttgggaactt gttcctcgcc gatgccgcaa atcctacgaa cgtcaggcga
     4921 ctgacaaatt ttccgaccga tcagatccga ccggtttcct ggactcggga cggcaagatg
     4981 gtcgtcgcgg agcgcgcgga caaagccggc atcgacctct ggctgtttcc cgtggacggc
     5041 agcgaaggga agccgtacct ggcaacgccg ttcgttgaat tgtcggctga tgtgtcgccg
     5101 gacggcacct acattgccta cgcgagcgac gagtccggaa gggatgaggt gtacgtcgaa
     5161 cgctttccat cgcacgccgg acgaaggcaa gtgtcatcgt ccggaggcga tcagccccgg
     5221 tggcgaaacg atgggcggga gctcttttac atttctcacg gcgaagttgt gggcgtggac
     5281 atgaccagca acacggccac tccgaagccg ctcttccggc tgcctggcaa ctcctacgat
     5341 atcgcccgcg atgggcagcg atttatcgtc gatcgcccac tcgacgacaa tttctcctcg
     5401 ccgctcacgt tcgtctccaa ttggatggcg gagcggaagt aggaactaag ccgccctcgc
     5461 cacaatcccg ccacattcct tgcgttggtt ctcctcatct gaccactcgt acgtcatatc
     5521 gcggcgccat attcgtcccg acggatcgaa ggagatggcg atggagagca gcgacacgac
     5581 cagcaccgaa caggaaccgc gggtcccgag gggaggggtg cgttccacgt cacaaaacgg
     5641 caacggctcc cgtgctaccc tccgccgcat gaagcgagct gttctgacga tcgtcctttt
     5701 tcttactgct gtttccgctt ttgctgccga caagaaccta accatcatca aggccggacc
     5761 gattggcgaa gttgccaatc tggccgaggc gaacgagatt cgcgtcgtct tcagcgagcc
     5821 gatggttgtc gtcggcaaga ttccgaaggc tctcgaagtg ccgtggtttc acgtcgagcc
     5881 ggcgatcaac ggctcgttcc ggtggtcagg aacgacgacg ctgatcttca cgcccgatcc
     5941 aaagacgccg ctgccgttcg caacgaagta cgacgtcacg gtcgacgctg ctgcgaaagc
     6001 ggtcagcgga aagacgctcg gcaaggcgta tcacttctcc ttcaccacgc cgacgatcca
     6061 gcttcttcgc accgactggt accgcaaagg cggcaagtac gacggcgcga tcgtcatcgc
     6121 actctggttc aatcagccgg tcgatggtgc aacgctcggc tcgcacctgg tgttgcgtac
     6181 ggtcgcgcac gaattcaaag agcccacgct tccggcgcgc gatcgcctgg cgaagctgga
     6241 gccggcgcag cccgccgcgt tcgacgcgaa gatagcgaag gcgcagcagg ccgcagcaag
     6301 cgacggacag ccggtgctct ccttcctggc gacggagtgg gacaagaaac acttcaagcc
     6361 ggtgccggag atggtcatcg tcgagacgaa gcccggcgtg ccgcctgaca cgaacttgca
     6421 gatcttcgtc gacgacaagc tggcgaagaa cgcgaacagc gttgccagcg ggcatccgca
     6481 aaccttcacc atcgagctgg gcccgacgtt cttcatcgag aaggctgatt gcctggcgga
     6541 gtgcggccct gactactaca acccgatctc gttccgcgtg aacggcggcg tgccgtacgt
     6601 gaacttcaag aaggcggtca gcgtcatcga cgtcaccgat ccggcgcacg agctgccggt
     6661 cacgccctcc gaaccgaagc gcgccgtcga tccgagcgcc gctcccgcga cgcagtacag
     6721 cctcgacgag ctcggatatt cggtgatccc cgcgcatacg tacgccgtcc gcgtcgatcc
     6781 ttcgctcaag tcggagaacg gccagacgct cggctacacg tggatggccg tcatcgagta
     6841 ctggcacaaa gcggcgtaca tcagcttcgg cgatggacac ggcgtgtggg aatcgagcgg
     6901 cggaccgatg ctgcccttcc acgcgcgcaa cttcaagaat gtcacgcagt ggatgacgcc
     6961 gttgtcgctc gacaagctcg tcccgacgat cgtcgatctg cagaaggaag gcttccacaa
     7021 aacgccggcg accggcggag cgcagcgcaa tctcgcgccc gttgcggaca agctgcagtc
     7081 gtacggcctc aatctcaagt ccgcgctctc atcgatcggc aacggcatcg tctgggctgt
     7141 catcaagcca ggcgacgcga tcgcgaagtc gaagacgtat tccgaggaca ccgctggcac
     7201 gatcgtgcag gtgacgaacc tcggcatcac cgtcaaggac tcgccgcaga acacgctcgt
     7261 cttcgtcacg cggctcgaca acgccgcgcc ggtggcaggc gcgaaagtct cgatccggac
     7321 gatcgagaac aaggtcttct ggaccggaac caccgacgcg aacggcatcg ccatcgcgcc
     7381 cgataccgat ctccgcgtgg agcggaacaa gaagaacgag aagggcgaag aagttccggt
     7441 cgagtcgtgg gaagcattcg gcctccactt cgtcgtcacc gctgagaaag acggcgacac
     7501 cgcatacgca tcgagcgact ggaacgacgg catcacgccg tgggagttcg gactcggctt
     7561 cgacctcgac gaagcgaagc cgcttctgcg gggcacgacg ttcacggacc gcggcgtcta
     7621 caagccgggc gaagaagtgc acgccaagat cgtgcttcgc agcgacaccc ccaagggcat
     7681 gcagctcctg ccggccggta ccgaagtcga catcgtcgtc accgatgcgc acgacaaaga
     7741 ggtcgacaag cgcacgctca agttgaatga ctggagcagc gcggagtggg tgtggaccgt
     7801 gcccgccgat ggcgtcctcg gcgactacgg catcaaagca tcagtcaagt cgcagcgcct
     7861 gatgacgttc ggcagtttcc tcgttgctgc gtaccgccgc ccggagttcc gcgtcgacgt
     7921 gacactcaag ggagcgccgc cgatcgccgg gacgaaactg aacggtacga tcatcggcaa
     7981 gtatctcttc ggcggcgcga tggcatcgcg gccggtgaag tggacgtatt cgaaagtccc
     8041 cgtttacgac gttccgtctg cgatccgcga gcggttccag gaagggcaat gggagttcct
     8101 cggcgaggat cccgagcaac gccgcgaagg ggagacgatc aacacccagg agccgaagct
     8161 cgacgccaaa ggtgaattga aactgacgct cgacaccgac atcgccgcgg gagtgccgtg
     8221 gagctacgtg ctcgaaggca acgtcactga cgtctcccgc cagcagctcg ccaaccgcac
     8281 cgcgttccgc gttcatccgg cgccgtggta catcggcgtg aagtcgccga actacttctc
     8341 cgacgccacg aaggggctcg acaccgccat cgtcgctgcc gcgctcgatg gcacggcaac
     8401 cgccggcgtc aaggtcagtg tcgaccttca tcgcattcag tggaactcgg tccgcgaggc
     8461 tgccggcaat ggcttctaca actggtcgac cgagcgcaaa gagcttcccg ccggacattg
     8521 ggatgtgacc acgcaggcga cgcctgcccc gctgcacatc cctctcggcg aaggcggcga
     8581 atatctgatc atcgcaacgg cgaaggatgc cgagggccgc tcgacgacga cacgcacgtg
     8641 gttctatgcg gtcggcggcg gctacacggc gtgggcacgt gcggacagca accgcatcga
     8701 gctcgtgccg gagaagaaga cgtacaagcc cggcgacacg gcgcggatca tggtgaagtc
     8761 gccgtgggag cacgcgacgg cactcctcac gaccgagcgc gaaggcgtgc gcacgtggaa
     8821 gacgttcgag ctgacctcga cgcagacgac cgtctccgta ccgatcaccg agaaagacat
     8881 tccgaacctc ttcgttgcgg tgacgctgct caaaggccgt acgaaggatg gcattgaaga
     8941 cgagagcgat ccgggcaagc ctgccttccg cctcggctat acagcgctga gcgtcgtcga
     9001 cgcagccaag cgtttgaagg ttgcggtgaa agcaaatcgc gacgagtacc ggccggcgtc
     9061 gaaggcgcgc atcgaagtcg atgtgcacga tgttgccggt aagccctcgc aatccgaggt
     9121 cacgttgtgg gccgtcgatt acggcgtcct ctcgctgacc gcgtacgaaa cacccgacgt
     9181 gctccagtcg atctatctcg agaaatcgct gcaggtcctc aacttcgatt cgcgccagaa
     9241 gatcgtcagc cgccgcgtgc tgacgccgaa gggcgagacg cagggtggcg gcggcggcaa
     9301 ggacgccgga ccgggcgtga tgcggaaaga cttccgcgtg ctcgcgttct gggtcggctc
     9361 cgtggtcacg gacgcgaagg gtcacgcgaa gaccgatgtc acgttgcccg agtcgctgac
     9421 gacgtatcgc atcatggccg tggccggcga ccgcggctcg cgcttcggtt gggcgcagaa
     9481 cgagatccgc atcaacaagc cggtgctgct gacaccgacg tggccgcgct tcctcgccgt
     9541 cggcgacaaa gcgttcttcg gcggcgtcgt gcattcgcag ctcaaacagc cgggcaaggc
     9601 gacggtaacg atcaagtcgc tcgatccgtc gatcgtcgag ttcacgcagc cgacgtcgca
     9661 gacggtggac atcgccgcgg agggttctgc cgaggtgcgg ttcaacgcgt cggcgaaagc
     9721 ggtcggcaac gcaagactgc agatgactgt ttcgctcaac aaagagaccg acgcgttcga
     9781 agactcgctg ccggtgcgca tcctcacgcc gtccgagacc gtcgccgcgt acggcgaggc
     9841 caagcctgac gcgaaggaga cgctggccgt acccaaggat gtcgtgccgt cgtccggcgg
     9901 actgcacgtc gagctgtcat cgacggcgat ggtcggcctg gaagaaggcg cttcgtatct
     9961 cgtcgactat ccgtacggct gcgcggagca gcgctcgtcc ggcgccatgg ctctcatgct
    10021 ctcctccgac ctcggcgaag ccttcgcgct tcccggcatc gacccgaaga agggccgcac
    10081 catcgcgcag gcgacgatca atgagctcaa caatttccag tgcggcgacg gcggcttcgc
    10141 gttctgggcc ggcgagtgct acaccgaatc gccgtatatg accgcgaccg tgttgcacgt
    10201 gatgcagcgc ggcgagaagt tgaagtacga cgtcaacaaa gacgtgacga agcgcgggta
    10261 tgactacctc gacgccaagc tcaaggaacc gcgtccgacg aacgaaggct ggtggccggc
    10321 gtacacggcg tggcaggcct tcgcggtgaa ggtactgacc gaaggcggac gaaacgacga
    10381 cagccacatc acgcggctct actcgtacgt cgaccggatg ccgctgttcg gcatctcgta
    10441 tctcgccgac gccatgctgg cgaagggaga gaagggcgcg cgcgtggagg atctgacgcg
    10501 ccgcatcacc aacggcatcc tgcccgaggg cggcagcgcg cacgtcgaag agctgtccga
    10561 tccctacctt ctttattact ggagctcgag cgttcgctcg accgccatcg ccctcggcac
    10621 gctcacacgc cacggcgaga gcgagcagat ggtgaagcag atggtgcgct ggctcatgaa
    10681 agtgcgccgt caaggacgtt ggggcaacac gcaggaaaac gtctgggcga tggagtcgct
    10741 cgtcgattac taccgtaagt acgagagcga gacgccggac ttcaccgcag tcgtgaagat
    10801 cggcgcggac acgatcgcca acgactcatt caaaggccgg agcgccgagg cgaagaatca
    10861 cgacgtccca atgcagcaac tgatggcgcg gccgccggga gacacgccgg tgacgttcga
    10921 tcgtcagggc acaggcacgc tcttttacct gatgcgcctg cgctacacgc cggccggtct
    10981 catgcacgat gcgctcaatc agggcttcag tgtcgagcgc aagtactcgc tgaagacgtt
    11041 caaggcgggt gacttgatca aggtcacgct gcgcatccgc aacacgaagg agcgccgctt
    11101 tgtggcggtg atcgatccga ttcccgcagg taccgagccg gtcgagtcgt ggttcgcgac
    11161 gaccgcgagc gatctggccg cggagcagag taagaacgat acgcagggcg atgactggat
    11221 gtcgtggtgg aaacacggcg gcttcgatca cgtcgagcgt cacgatgacc gcgtcaacct
    11281 tttcgccacg cgcctcggcg aagggatgca cgagttcacg tacatggttc gcgccacgac
    11341 tgcgggaaca ttcatcaccg cgccgacgca tgccgaggag atgtacgagc ccgaagtctt
    11401 cgggagaacg gccagcgagg tcatcgaggt caagccgtga agttcatgaa agtggagcgc
    11461 cggcggctcg ccggctggac cgccggcggc ctcgccggcg gtttactcgc tctcgcgctc
    11521 tgggttcgcc taggtccaat cccgacgatc acccccgaca cctcgcccgt catcgtcgac
    11581 cgcaacggcg tggtcttgta tgagccgctg tcgccgagtg gtacgcgaaa cgagtggatc
    11641 aatgctgtac cggagtcgat cgcggccgcg accatcgccg ccgaggatcg ccgtttcgag
    11701 catcacccgg gcatcgatcc ggtcgcgatc gcgcgcgcgg tcgcgcacga catccgccat
    11761 ctgcgcgctg tcgaaggagg ctcgacgatc acgcagcagg tggcgaagat gctgttgggc
    11821 aatccgccga gaacgatcgt gtcgaagttg cgcgaagccg tcatcgcgct ccgcctcgag
    11881 catcgctaca cgaagcgcca gatcctcgcg ctgtacgtga atcttgcgcc gtatggcgag
    11941 cagacgatcg gcgtcgcccg cgccagccgg cgctacttcg gatgcgcgcc ggaagagttg
    12001 acgaccgcgc agtcggcgtg gctggcgtca ttgccgcaac gtccgtcgac gccacgccgc
    12061 gcgttggcaa ggcagcgcgt cgttctcgcg cgcatgcact cgctgggatt gatcaacgac
    12121 gcctcgtggc gcgaagcgcg cgccgaacgg ttgacactcg atcgcggtat ccaaccaacg
    12181 ctcgctcctc attttgtcga acaggttcgg agccggtcgg gcgcgcaacc gaatcacctc
    12241 gtgaagacaa cgctcgacgc cgtgctgcag cagaacgttc gcggcatcat cgcggcggag
    12301 cgcgcgaacc tgctgcgtca cggcgcgcac agcgtggctg tcgtcgttct cgacaacgag
    12361 acgggcgggt ggttggcgtg ggaaggttcg ggcgattact tcgggaccga tttcggcggc
    12421 gcgatcgacg gcgtgacgac gttgcgtcag cccggatcga cgctcaagcc gttcacctac
    12481 gcgctggcgt tcgaagaggg gatcacaccc gcgaccgtgc tcgcggatgt gccttcgcac
    12541 tttccgaccg ccgaggaagg agtcgtctac acgccgcgaa actatgacgg cggctatcgc
    12601 ggtccactgc gtgcgcgtgc cgctctggcc ggatcggaaa acgttcccgc cgttgcgttg
    12661 ctggagaaag ttggccctcc tgcgttgctg cgcatgctgc gcggcactgg ttttcgcgac
    12721 cttacccaca ccgctgatta ctacggtctc ggactcacgc tcggcgacgc cgaggtgacg
    12781 ctcgagcagc tcgtgagcgc gtacgcgacg ttcgcgagag gagggacgtc aatcgcgacg
    12841 acgatgttgc tccggccccc ggcccccgat tcgcgacccc cgcgcatcgt ctccccgcgc
    12901 accgccttct ggatcaccga catcctcagc gactcgcacg cccgcgagtt catcttcggc
    12961 cgcggcggca atctcgattt tccgtttccc gtcgccgtga agaccggcac gtcgcaggcg
    13021 taccacgaca actggacggt cggctacacg cgcgcggtca ccgtcggcgt ctgggtcggc
    13081 aacttcgatc gccaggcgct gcgcagctcg agcggtgtca ccggcgccgg accgatcttc
    13141 cactcggtca tgctcgcggc aatgcgccgc gccggcgaca acggcgagcg cgagatcgtc
    13201 gaccgcccgg atgacctgga gcagcagccg atctgcgcgc tgtcaggatt gcggccatcg
    13261 acatcgtgcc ctgcgatcga gagcgaatgg ctgccgcgcg acgcgcccgt cgagttctgc
    13321 gcatggcatc acgcgggccg gatcgactgg cccgaggagt accgcgattg ggcctcagca
    13381 cgagaacgcc gcacgccggc gtccgcccca cgtgcactgg ccggacgtga aacacgattc
    13441 cgcatcacca gcccatccaa cgacaccacg tacctcgtcg atccaaccct ccaccgcgag
    13501 tttcaggcga tccacctgcg cgcaacgagc gacgcaacct ggagcgtcga cggaagggcg
    13561 gtgtcgagcg agtggccgtt gcgaaccggc aagcactcga tcgtcgcgac gaatcgccgc
    13621 ggtgagcgcg acgcggtggc gatcacggtg cgataatctc tcagagaatc atcaaccaca
    13681 gagacacaga gatcacagag gttttctctg tgtcctctga gtctctgtgg tgaaggattt
    13741 tgtgaaatga caacagcgac cgtgacgacc aaaggacgag taaccatacc gaagagtgtc
    13801 cggaatctac tgggcatcaa gtctggagat caggtcgact tcgtgatcac cggtcgcggc
    13861 gacgttgtcc tgcgggatgt gggcggcgat attcgtgaat tgcgaggcat gctgaaacgt
    13921 gatcgccagc ggagtgtgac cgtgcgcgag atgaacgcgg ccgttctgcg tcaccacgca
    13981 aggaatcgtt gactcatcgt gcctaccgtt ttgcgcaccg gtccgtaccg ggtgtacttt
    14041 tata
//
LOCUS       DATLVW010000191         3278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_296361, whole genome shotgun sequence.
ACCESSION   DATLVW010000191 DATLVW010000000
VERSION     DATLVW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296361"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1088
                     /locus_tag="VNN25_06110"
     CDS             141..1088
                     /locus_tag="VNN25_06110"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXH91135.1"
                     /translation="MISSKRFTTFSFAAFLILLGATLATAQQTQYRPIYPSQKASVMQ
                     TIGVTDVTITYYRPAIKGRTIFADAPPEMEARAKGEATLDNQNERKAGEPIVPYNHVW
                     RAGANDATVFQVTDDVLINGQPLKAGTYSLSTIPGKDEWTIIFNNDYGQWGAFSYDAK
                     KDALRVKAKPQPVAQSQELLAFNFDPVTANTATANLRWEKVNVPFTVEVKDVKALWRA
                     KTDALIAANPANEVFPLQAANTFVADKNWDEALKYVDQSLKTKPTFRGLSAKANILHN
                     AGRKEESLAVADQAIAKGKADGVDTSAFEKRVAGWKSGT"
     gene            complement(1092..1538)
                     /locus_tag="VNN25_06115"
     CDS             complement(1092..1538)
                     /locus_tag="VNN25_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91136.1"
                     /translation="MKNPATYDDANLILKLYDLRREPRMREARKWFGGAPQFQSREEW
                     LKCCPPGSEENASYRMVVTYWEMVASLVASDVLNAELFYASQGEMLFVWEKIRILVPE
                     VRAVQKNPTLYRNLEQVATAFIEHMKAGAPDWYDGFFGPMIAKAGR"
     gene            1667..1945
                     /locus_tag="VNN25_06120"
     CDS             1667..1945
                     /locus_tag="VNN25_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91137.1"
                     /translation="MAFVVRFTPPSLSAQQYDEVIKRLEAAGEGSPKGRLFAVAFGPT
                     DALRVSDVWDTQENFDRFAQTLMPLLQELRIDPGTPEFIQAYHVNPGA"
     gene            2085..2561
                     /locus_tag="VNN25_06125"
     CDS             2085..2561
                     /locus_tag="VNN25_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH91138.1"
                     /translation="MKSKNTICLWFDKDAAEAARFYAATFPDSEVTAVHKAPGDYPGG
                     KKGDVLTVEFTVVGIPCLGLNGGPTFKHSEAFSFQIATDDQEETDRYWNAIVNNGGQE
                     SQCGWCKDRWGLSWQITPRALTDALAAGGDEAMRAFAAMMPMKKIDIATIEAARRG"
     gene            complement(2591..>3278)
                     /locus_tag="VNN25_06130"
     CDS             complement(2591..>3278)
                     /locus_tag="VNN25_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH91139.1"
                     /translation="EPQQERSRESLRKLLKAATEVLGQHGVEGTTIPRIAQHAGLTPG
                     AVYRRFRDKDELIETAILGILERQDERVKVGMTPEMAARIPLDVFAEQIIGGMVVSYR
                     ANAALLRALRQFVQRQANTAFVKKAAKLEVRTYERLVDRILAGRTDIRHPNPRLAVSL
                     GLMMVISTLYELVVMPTTPIDWKGLLPKDDHTLKRELTRAFLNYLDVGATPSTAPPTT
                     SARRKSRRKS"
BASE COUNT          662 a         1010 c         1013 g          593 t
ORIGIN      
        1 cgcatgacgc cgcgctgtcg atcagctcag ctcgttcgat gattcttctg tagcggtcac
       61 gtcacagctt gtcagcgccg ctggaaccgg ctgcggttcg tagataatgt gcgatcacgt
      121 tcgaaacgaa ggagcaccgc atgatctcca gcaaacgatt cacgaccttc tcgttcgccg
      181 ctttcctgat cctcctcggc gcgacgctcg ccaccgcgca acagacgcaa tacaggccca
      241 tctatccgag ccagaaagcc agcgtcatgc agacgatcgg cgtcaccgac gtgacgatta
      301 cctactatcg tcccgcgatc aaaggccgca cgatctttgc cgatgcgccg cccgagatgg
      361 aggcgcgcgc gaagggcgag gccacgctcg ataaccagaa cgagcgaaag gccggcgagc
      421 cgatcgttcc gtacaaccat gtgtggcgcg ccggcgcgaa tgacgcgacg gtctttcagg
      481 tcaccgacga tgtcctgatc aacgggcagc cgttgaaagc aggaacgtac agtctttcga
      541 cgatccccgg gaaagacgag tggacgatca tcttcaacaa cgattacgga cagtggggcg
      601 cgttctcgta tgacgcgaag aaagacgcgc tccgcgtgaa ggcgaagccg cagccggttg
      661 cgcagagtca ggaattgctc gcgttcaact tcgacccggt gacggcgaat acggccacag
      721 ccaatctgcg ctgggagaag gtgaacgtgc cgtttaccgt cgaagtgaag gacgtgaagg
      781 cgttgtggcg tgcgaagact gacgcgctga tcgccgcaaa tccggcgaac gaggtcttcc
      841 cactccaggc ggcgaatacg ttcgtagccg acaagaattg ggacgaggcg ctgaagtacg
      901 tcgatcagtc gctcaaaacc aagccgacgt ttcgaggtct ctcggcaaag gccaacatcc
      961 tgcacaacgc gggcaggaag gaagaatcgc tcgccgtcgc cgatcaggca atcgccaaag
     1021 ggaaagccga cggcgtcgac acgtctgcgt tcgaaaagcg cgtggccggc tggaagagcg
     1081 ggacgtagcg gttatcgccc ggccttggcg atcatcggcc cgaagaatcc gtcgtaccaa
     1141 tcgggagcgc ctgccttcat gtgttcgatg aaggcagtcg ccacctgctc gagattccgg
     1201 tagagcgtcg gattcttctg gaccgcacgg acctcgggca cgaggatgcg gatcttctcc
     1261 cagacgaaga gcatctcgcc ttgcgacgca tagaagagct ccgcgttcag cacgtcgctg
     1321 gcgacgagcg aggccaccat ctcccaatac gtcacgacca tccggtagga ggcgttctct
     1381 tcgcttccgg gcgggcagca cttcagccac tcttccctcg attgaaactg cggtgcgccg
     1441 ccgaaccatt tgcgcgcttc gcgcatccgc ggttcgcggc gcaggtcgta gagcttcagg
     1501 atgaggttcg cgtcgtcgta cgtggcagga ttcttcatag cccgcgaact ctatcgcgag
     1561 cactcatcgc ggcaaaggga agtctcatga cggccgaaaa tggatacatc caaacgaccg
     1621 gacgcaacgt atcttcaagc gacttcagag gaagagggaa ccaaacatgg ccttcgtcgt
     1681 tcgctttacc ccgccgtcgt tgtccgctca gcagtatgac gaggtcatca aacgcctcga
     1741 agcagccgga gaaggttccc ccaaaggacg gctctttgcc gtcgcattcg ggccaaccga
     1801 cgctctccgc gtttccgacg tctgggacac gcaagagaat ttcgaccgct tcgcccaaac
     1861 gctgatgccc ctgctccagg agctccgcat cgacccaggc acgccggagt tcatccaggc
     1921 ctaccacgtc aacccgggcg cgtaacggga cctttcgaac cattgcacca cagagacgca
     1981 gaggactcag agtggaatct actctgagtc ctctgtgtct ttgtggtgca ttcttgtcca
     2041 accgatcgaa ggtaacctga tcgagaagaa gggagcagac gaacatgaag tcaaagaaca
     2101 cgatttgtct ctggttcgac aaagatgcgg ccgaggcggc gcgcttctac gccgccacct
     2161 tcccggatag tgaagtgact gccgttcaca aggcgcccgg cgactacccg ggcgggaaga
     2221 agggcgatgt gctgaccgtg gagttcaccg ttgtgggcat tccctgcctc ggtctcaacg
     2281 gcggcccgac gttcaaacac agcgaggcct tttcattcca gatcgcgacg gacgatcagg
     2341 aagagacaga ccgctactgg aatgcgatcg tcaacaacgg ggggcaggaa agccaatgcg
     2401 gttggtgcaa ggaccgctgg ggcctctcct ggcagatcac tccacgcgcg ctgaccgacg
     2461 cgctcgcggc cggcggcgat gaggcaatgc gtgccttcgc agcgatgatg ccgatgaaga
     2521 agatcgacat cgccacgatc gaggccgcgc ggcgcggctg acggaagtgc gaccgaacgg
     2581 ttccgcgccg tcacgatttg cgtcgtgact tgcggcgtgc cgatgtcgtg ggtggggcgg
     2641 tgctgggcgt cgcgccgacg tccaggtagt tcaggaacgc gcgtgtgagc tcgcgcttga
     2701 gcgtgtggtc atcctttggc agaaggccct tccagtcgat tggtgtggtc ggcatcacga
     2761 ccagctcgta cagcgtgctg atgaccatca tcagccccag cgagacggca agccgcggat
     2821 tggggtgcct gatgtccgtg cgcccggcca ggatgcgatc cacgagccgc tcgtacgtgc
     2881 ggacttccag cttggcggcc tttttcacga atgccgtgtt ggcttggcgc tggacgaact
     2941 gccgcagagc gcgcagcaac gcggcattcg cacggtacga cacaaccatg ccgccgatga
     3001 tttgctcggc gaagacgtcc agggggatcc gggccgccat ctccggcgtc atgcccacct
     3061 tgaccctctc gtcctgccgt tccaggatgc cgaggatcgc ggtctcgatc agctcgtcct
     3121 tgtcgcggaa tcggcggtag accgcgcccg gcgtgaggcc ggcgtgctgc gcgatgcgcg
     3181 gaatcgtcgt tccttcgacg ccgtgttgcc ccagcacttc ggtggcggcc ttgagcagct
     3241 tgcgcagcga ctcccggctg cgctcctgct gtggctcg
//
LOCUS       DATLVW010000192        16814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_7101, whole genome shotgun sequence.
ACCESSION   DATLVW010000192 DATLVW010000000
VERSION     DATLVW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7101"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..443
                     /locus_tag="VNN25_06135"
     CDS             165..443
                     /locus_tag="VNN25_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91140.1"
                     /translation="MSFVVRFVPKSMSSAQYDQVIKQLEAAGAGSPRGRIFHVAFGPK
                     DALRVSDIWDTHENFDRFGQVLMPILQAAGVDPGEPEFIEVHNVVKGT"
     gene            476..1390
                     /locus_tag="VNN25_06140"
     CDS             476..1390
                     /locus_tag="VNN25_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91141.1"
                     /translation="MSPSFWAGRAVRRILSGTHRHVKGTSAPAGDLMKVRTVVALLFL
                     CSSVAAFGQQGASHGSFIGHFFGYGFLFQVAALIHWARKRPDTFWIFIIIIGGMIGSI
                     AYFLIEGLPDFRNVQRTLRGPSRRKRIEVLRVLVIENPSPGNYEELGELLLEDKRYHE
                     ARDAFDRALAVRTDSIDPFYRRGIAEFELRDDEAAVNDLRHVTAADPKYDFSRAACLL
                     GQALARLGRNDEAMAVFDKFAQNATGAEPLVSAAEFYASHGRSAEALEIAQNIAMREM
                     TMPAYQKRRDRVWLRRAKALERTLQKKA"
     gene            complement(1404..1832)
                     /locus_tag="VNN25_06145"
     CDS             complement(1404..1832)
                     /locus_tag="VNN25_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH91142.1"
                     /translation="MTLKFLLDTNIVSEPLRPRPSAGVLRRLSRHEEELAIPSLVWHE
                     LRFGVERLPPSRRRSTIERYLEEVVLVTMPILDYDSVAAAWHASERARLAARGETPPF
                     IDGQIAAIAHVHDLALVTFNVADFKRFDGIRVVSWALTSR"
     gene            complement(1829..2110)
                     /locus_tag="VNN25_06150"
     CDS             complement(1829..2110)
                     /locus_tag="VNN25_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXH91143.1"
                     /translation="MKEQHSIAEARSNLPQLVRKAESGKPVELTRRGESVAVLIGRKQ
                     YERLVSKSRRFSEAWDAFASEVSLADLQIDPDEVFADVRDRAPGRDTAL"
     gene            2373..2696
                     /locus_tag="VNN25_06155"
     CDS             2373..2696
                     /locus_tag="VNN25_06155"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HXH91144.1"
                     /translation="MKKALFLFLLLVAISPLAYPSCSTLDITDESLGPMSAGTPFSHQ
                     MNACCGTAPYTWSIASGTLPPGLSMTSGGLISGTPTTSGSFFVCIKVTDAASCSLTRC
                     YDLEP"
     gene            complement(2704..6591)
                     /locus_tag="VNN25_06160"
     CDS             complement(2704..6591)
                     /locus_tag="VNN25_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH91145.1"
                     /translation="MRAVFLAISIFVSSFAAWGAITGTVVGGDGRAIASAKVAAFALE
                     TSEEQRVRWVSADPARKPLVTGATDANGRFSIDPKATVVDLRVEVSGQPAIGVRSANG
                     EDAGVLQAPSQPLVRGTVTANGKPLPDATVIVRSGGAEGVATTDAHGQYWLPDPKKLV
                     SWILVQHTGYAPVEREIDTVALRNPDVAMTAGIVLNGRVVAADGETPVAGASIQIDDL
                     ILAKTGADGTFAIEHAPAGARRIAARAGDRISARLIGGARQFLFRLGSAAAISGSIRD
                     IKSGIAIAGAQVTAAAPRYGGLDPGAWSVTDDKGNFTIAGLAGGEYELNAVHPGYATP
                     RLVVNAPPGVTVRKTLYAAALARISGSVIDEGARAVAGVSISTRRVGGDMLWTPSSVR
                     MTSRAITAPNGHFILRIAEDGNVQLDATKTGMPAAHSGIIRVAPGSRTADIVITLPRG
                     VALAGRVIAPDAKPVSGAAVTATEPGAGGVRDAEEGVRTKADGTFAVRVRGGTYDVTV
                     TAAGFAPRTVRTQVTASTPPLEVTLEAGVEISGRVTCGETPVDGVDIFIISGGDAPPV
                     QTSADGRFRIIDLAPGQVTLAFKKPTELIQATRTVTAPAADVNVEVPAGGRIAGRVID
                     KGTSQPVTSFDAGITRGSAALMRTPAMRAFTSDDGTFAIDGAPVGSQTLSVTAPGYVM
                     ARVPNINVESGKSVEGIEVALDHGVRVSGHVTGPDGGPAGGVLVRVDPTTSTRGATTN
                     DSFTLTDPDGAYALDSLDAGPTTLAFSRSGLVTVRKSVTLSGSSSEVDAQLGAGSSIA
                     GVVVLDGGAPVAGADVRAFSATDTAGMSSQTDEGGTFVIAGAPAGHYEISATRAGYGS
                     ATLRDVEIPAPSMLRLVINRGGVITGAITGLTRAELQSASVQAWSADGATASASPDES
                     GHYRIEGAAAGSVRVGASVKPLPGSARNAVTKVVQLDAGATVNVDFDFTAEIIINGRI
                     TRNGRPLPGVEVSFAPQSAALRYARSAADGNGRYEISGIDNGSYAVTVYDTEQGPYST
                     VYEVKGSSTFDIDLRGVRVAGRVSDATTDAAIANARVDLLRTDGNASDVRSTLSDASG
                     GFSFDQVAAGRFEARVQKASYGAAAVPVTVGQDGVPPLEVKLSPSQGVAIRVIDARDG
                     QPLNGWYHAESAAGESYDGAIAGTAEPAPIALAAGSYRITIGAAAYAPRTMIIVAPGE
                     QTVALTPGGTIVVSSSSESVAALRIIDAAGQPVHFGPGPAAGMVRLDPAPGQTRIANV
                     AAGTYTLQIVADGRVLRSALVTVREAETVSAKL"
     gene            6762..7076
                     /locus_tag="VNN25_06165"
     CDS             6762..7076
                     /locus_tag="VNN25_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH91146.1"
                     /translation="MPQIAIDYDKEKLRDFCRKWKITEFSLFGSVVREDFGPESDVDV
                     LVAFAADAHWSLFDMSDMNDELKILFDRDVDLLTRRAVEGSLNPIRKTSILSSAVMLD
                     VA"
     gene            7066..7398
                     /locus_tag="VNN25_06170"
     CDS             7066..7398
                     /locus_tag="VNN25_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXH91147.1"
                     /translation="MLRESGFLQDILLSARLAISYLERTSVEEFAKDVQLQDSVIRRL
                     EIIGEAASHIAEEAATELPELPWRKMVDMRNFAIHQYWDVDVNIVWETVRNDLPAAVA
                     AIERYLAL"
     gene            7557..7868
                     /locus_tag="VNN25_06175"
     CDS             7557..7868
                     /locus_tag="VNN25_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH91148.1"
                     /translation="MPHIAIDYDKEKLRDFCRKWKITEFSLFGSVVREDFGPESDVDV
                     MVTFEKGAPWTLFSLVHMEDKLAEIFRRPVDLCERPGVERSRNRYRRESILSSAVALE
                     L"
     gene            8124..8939
                     /locus_tag="VNN25_06180"
     CDS             8124..8939
                     /locus_tag="VNN25_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH91149.1"
                     /translation="MLPEKTFEGRVGIITGGGTGIGFAIARELAKLGATVILASRSAE
                     HLGPAVEQLRSETGRANAADFIALDVREADAVEAMAAKVHGDHGHIDFLVNNAAGNFI
                     APADQLTVNGWNSVIGIVLNGTFYCTSAVGKRMIAAKKGAILNVIANYAWTGAPGVVH
                     SASAKAGVLAMTQTLAVEWARHKIRVNAIAPGPVHTEGASKRLFPNEMIEEGIRKTIP
                     LRRFASLEEVANAASYLLSDYASYVTGEAMVIDGGQWLSGADYWTRVQQMAPK"
     gene            9016..9771
                     /locus_tag="VNN25_06185"
     CDS             9016..9771
                     /locus_tag="VNN25_06185"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HXH91150.1"
                     /translation="MQGVTSPEWSTFLGTVKTANDELGDPEVVWYRGHPDADHYLLAS
                     LLRYENGLTKEKYLFESFQKFSDRIFKRRISEWETLFEMQHYGVPTRLLDWTESFGVA
                     LFFAAYYSKRRALTNDAAIYLLSPHRLNKYSGINKVYRIPEDEGNFSYKGIYWNRTPF
                     AATAPIAIEPIFINDRMLAQRGMFTVHHDEINPIETEFPAAIKKVVIPNNVVEAAMDF
                     LDLTNINEYSVFPDLAGIAGYLKSESDLAPRWP"
     gene            complement(9824..11119)
                     /locus_tag="VNN25_06190"
     CDS             complement(9824..11119)
                     /locus_tag="VNN25_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXH91151.1"
                     /translation="MRLLAILALEVLMTATLDAATTLDQRIDDAAKKVEAKVIACRRD
                     IHQHPELGNRETRTSKIIADRLRELGIEVKTPIAHTGVIGILRGGKPGRVVTLRADMD
                     ALPVVEQVDLPFKSTVRTTFNGHEVGVMHACGHDAHVAILLGVAEVLSGIRDEIPGTV
                     KFLFQPAEEGAPEGEEGGAKLMVKEGALDNPKVDAVFGLHVTSRYPVGEIAYRSGSMM
                     AAVDSFKITVHGKQTHGAYPWLGVDPIVIASQIVLGLQTIPSRQVDSSLAPSIVTVGA
                     IHGGVRNNIIPDDVEMIGTIRSLDAKMRDEIHARIKRTAEDIARSGGGTATVSIEEGY
                     PITYNDPAITEQMAPTLRRIVGPANVEVVNATLGAEDFSFFQQKVPGLFFWLGTRPKN
                     QTAEEAASNHSPLFYVDESGLELGVRAMAHVAVDYLKKP"
     gene            complement(11233..11520)
                     /locus_tag="VNN25_06195"
     CDS             complement(11233..11520)
                     /locus_tag="VNN25_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91152.1"
                     /translation="MSDDDQRSDEARETFAYFSNEYAQALQAFKAIEEQSSTLMLMGV
                     ADDLRGFIEQFIAMASGTKALAEDKGETHFAEWFGELIHKAEALKTEIVSQ"
     gene            11624..13345
                     /locus_tag="VNN25_06200"
     CDS             11624..13345
                     /locus_tag="VNN25_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH91153.1"
                     /translation="MRRWRCLLLLSIACSISSLAQTPVVPHAGETIEVSIVNIDTVVT
                     DKHGNRVRGLGKDDFEVYENGARQPLTNFAEYGSEGKASSVGTVTAPAAEGAPRSAPQ
                     PPPSQPRTIIVFVERFQLPSFRSDPVFAAMKKLLHETVRKGDRVMVVTWNRGILGMRQ
                     QFTDDLVRIDKAIEMIADRTTTLNHDPIADLEQEIDSIQAFEEETAAMNGGRIATSNI
                     TNESGLSFFVEQMARFTAKDAYLDEKRKVETISTLMRSAGGTEGKKVLLLATHRLSMY
                     AGAEALFAAGAPLLPADFKNQFDGKQLVRSLIEAANQTGFTIYPIYAEGLAMTSGATS
                     ELRARQNKPIGYEYLVLNNETPMLEYVAKETGGSAAWGSNDVTKLIPTVGEDLDSYYS
                     LGYRATPGKAAARKIEVKVKDPKLVVRARTQYMPKSDTARMDERVIATLFGNQPPASF
                     EVKVRLAPPKLDEQKRYIIPVTIEVPISALTTLPAAGNVHNGAFTVYMAWGAVLGGLS
                     EPHRDTKQFSIADADLAKAKGNYFTYQVGVASATPYLRIALGVYDEVSRDYALNLIDL
                     EPPMKKK"
     gene            complement(13664..13987)
                     /locus_tag="VNN25_06205"
     CDS             complement(13664..13987)
                     /locus_tag="VNN25_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HXH91154.1"
                     /translation="MITNDEERLRASLTFTESTIPDRIAADSDIVENGLAKLVLSIIE
                     LIRRLLEKQALRRMDAGTLTDEEIERVGAALMKLEEKMAEMKQIFGLADDDLNLNLGP
                     VRSLD"
     gene            complement(13974..14615)
                     /locus_tag="VNN25_06210"
     CDS             complement(13974..14615)
                     /locus_tag="VNN25_06210"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HXH91155.1"
                     /translation="MKLVVIGAHLERVDIEPVAQPLAVGDLWLSGMPVADDQPLGDRA
                     LLLRVAQTRALLLDRGTFIAIRYGFTAHASSDAMTKCAAHLERWRRTLQECRGMVEMT
                     LKVAAASPQARPDRHDFASGAAYFRALHDATQAASIDPLFRDAVEKNLTPLAVKHRWS
                     NRDNASLEFALLVRADSVAAVNDAGAELKRGFASVPFLLSGPWPLEVFADDHE"
     gene            complement(14612..14800)
                     /locus_tag="VNN25_06215"
     CDS             complement(14612..14800)
                     /locus_tag="VNN25_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HXH91156.1"
                     /translation="MSDENDELSLLETLDHLLDRGVVIAGEAVVSIGDVDLLYLGLNV
                     VLANVDAIIRTSQPKRIK"
     gene            complement(14967..15749)
                     /locus_tag="VNN25_06220"
     CDS             complement(14967..15749)
                     /locus_tag="VNN25_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HXH91157.1"
                     /translation="MPEVLYVYAIGRAGRPLPERLEAIDGSDRLQEVTAGALAAFVTP
                     VDDVDFSQAVIDARAKDVEWLGGIGYRHQSVMSALMHSGTIIPLRAFTLFASAETLRA
                     HLEAEHARLSTLLDRLEGKQEWTLRLEFDPERWSEALVRRVDSLRVLSEEIDGAAAGK
                     AFLLRKKLDDEKKRASREAEQQVMAEVESAVLEKLACDTVAETRQQRSGAFPQINVLI
                     NRDEESRLVELREALSQRYGSEGVTLALTGPWPPYTFVGREE"
     gene            complement(15774..>16814)
                     /locus_tag="VNN25_06225"
     CDS             complement(15774..>16814)
                     /locus_tag="VNN25_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HXH91158.1"
                     /translation="HNTPLWIVSAGFHRPGDIGDWSCQFPVVLASPLESFRLHYAPGI
                     QHLLLNRLTAADTRALVARYPSHVMHLGAENAFFLGGGWYGPEGESGDLSRWTGPEPV
                     SVMLIASASIDHRLLLRMAPFDFRGAPPQVATISLNGAPLAQITLVRDWKDYPFDVPR
                     ARWRDGVNFLTVAFSRANIPASVDPNSRDTRPLAGRFNLIAVMPAVSALKLADSRPEL
                     TRAFRINEPPAELLDENAAWLGVPQKTRALEKPQLQFLLGRLGFDPQAAMTKLQRGKV
                     DLGELAETLAYNSACLNDTDFIRLAYATILDRPADADGQRYFESALKKRETRVGVVRA
                     LIDSDEVRRTSP"
BASE COUNT         3161 a         5381 c         5163 g         3109 t
ORIGIN      
        1 ctttttgaac caacgacgct aggacttgaa gcatcgacgg tgcttcttga tccgccgagg
       61 ctaggtttcg aaccatcgat ggcgcgctct ctttggttcc agaaggattg tccaatccga
      121 tcaccctccc gcgtcgtaac atcgccggaa aaaggagagc ttccatgtcg ttcgtcgttc
      181 gctttgttcc gaaatcgatg tcatccgcgc agtacgacca ggtcatcaaa caacttgaag
      241 cagccggtgc cggctcccct cgtgggcgca tctttcacgt cgccttcggt cccaaagacg
      301 cgctgcgcgt ttccgatatc tgggacacgc acgagaactt cgaccgcttc gggcaggtgc
      361 tgatgccgat cctgcaggcc gctggagtcg acccgggcga acctgaattc atcgaagtgc
      421 acaacgtcgt caaaggcacc tgaaacccgt tcctttcgcg aacgtttcta cagcgatgtc
      481 cccatcgttc tgggctggcc gcgccgtccg gcgtatcctg tccggcacac atcgccacgt
      541 gaaaggaacg tccgctccgg cgggcgatct gatgaaagtc cgcaccgtcg tcgctctgct
      601 gttcctctgc tcctcggttg ccgccttcgg gcagcaaggg gcatcgcacg gctcgttcat
      661 cgggcacttc ttcggttacg gattcctctt ccaggtggcg gcgctcatcc actgggcgcg
      721 caaacgcccc gacacgttct ggattttcat catcatcatc ggcggcatga tcggatcgat
      781 tgcctacttc ctcatcgaag ggctgcctga ttttcgcaat gtccagagaa cgctgcgagg
      841 cccgtcgcgc cggaaacgga tcgaggttct gcgcgtgctg gtgatcgaga atccgtcgcc
      901 cggaaactac gaggagctcg gcgagctgct gctcgaggac aagcgttatc acgaagcacg
      961 cgatgccttc gaccgcgccc tggccgtacg caccgactcg atcgatcctt tctaccggcg
     1021 cggcatcgcc gagttcgagc tgcgtgacga cgaagccgca gtcaacgatc ttcgtcacgt
     1081 caccgccgca gacccgaaat acgacttctc ccgagcggcc tgtctgctgg gccaggcact
     1141 ggcccgactg ggccggaacg atgaggcgat ggcggtattc gacaagttcg cgcaaaacgc
     1201 cacaggcgcc gaacccctgg tcagcgccgc ggagttctac gcaagccacg gacggtctgc
     1261 cgaagcactc gagatcgccc agaacatcgc catgcgggaa atgaccatgc ccgcctacca
     1321 gaagcgccgc gatcgggtgt ggcttcgcag agcgaaggca ctggagcgca cgctgcaaaa
     1381 gaaggcgtga gcgcgtcgta ccctcacctt gaggtcagcg cccagctcac cactcgaatc
     1441 ccgtcgaatc gtttgaagtc cgcaacgttg aacgtgacca gcgcgaggtc atggacgtga
     1501 gcgatcgctg cgatctgccc gtcgatgaac ggtggcgtct ctccgcgggc cgcgagacgc
     1561 gcgcgttctg atgcgtgcca cgctgccgcg acgctgtcgt agtcgaggat cggcatcgtc
     1621 acgagaacga cctcctcaag gtaccgttcg atagtcgaac gacggcgtga aggggggagc
     1681 cgctctactc cgaatcgcaa ctcgtgccac acgagcgacg gaatcgcgag ctcttcttca
     1741 tgacggctca gccgccgcaa aacaccagcc gacggcctcg gacgaagcgg ttcggaaacg
     1801 atattcgtat cgagcaggaa cttcagcgtc acagcgcagt gtcccgcccc ggtgcgcggt
     1861 ctcgaacgtc tgcgaatact tcatcaggat cgatctgcag atccgcaagc gagacctcgc
     1921 tggcgaaggc gtcccatgct tccgagaatc gccgcgattt cgagaccagg cgttcgtact
     1981 gctttcggcc gatcaaaacc gccacactct cgccacgcct tgtcagttcg accggctttc
     2041 ctgattccgc tttacgaacg agctgcggca gattgctgcg cgcttccgcg atcgaatgtt
     2101 gttccttcac tctcatctca tgtacatcga catgtacata gtagcacgag aaacaaaacg
     2161 cggtgatcct cggactgcct gattcgcttt gaatgcagac tcagccattt aactgacggc
     2221 caccactccc atgcgtaacg cgggatgcgc actttccaaa actgatactc ccctccggac
     2281 gtcggcggac ttatattgaa ggccttattg acaggcggcg gtctccgtgc cccggtcggc
     2341 gctggtttgt cgtcgccaaa aaggaggtac ctgtgaagaa ggccctattc ctctttctcc
     2401 ttctcgtcgc gatcagcccc ttggcgtacc cctcgtgcag cacgctcgac atcacggatg
     2461 agagtctcgg ccccatgtcc gccggcacgc ctttcagcca ccaaatgaat gcctgttgcg
     2521 gcactgcacc gtacacatgg tcgattgcct cgggaacgct gccaccgggc ctgagcatga
     2581 ccagcggcgg cctgatctca ggcactccta cgaccagcgg ttctttcttc gtctgcatca
     2641 aggtcaccga tgcagcgagt tgctcgctga cgaggtgcta cgacctcgag ccgtagtcag
     2701 gaatcagagc ttcgcagaaa ccgtctcggc ttcacgcacc gtcaccagcg cgctgcgcag
     2761 caccctgccg tcggcaacga tctgcagggt gtacgtcccc gcggcgacat tggcgatgcg
     2821 cgtctgaccg ggcgcggggt cgagacgaac catccccgcc gccggacccg gcccgaaatg
     2881 aacgggctga ccggcggcgt cgatgatgcg aagtgccgca accgactcgc tgctcgacga
     2941 cacgacgatc gtcccgccgg gagtcagcgc cactgtctgc tctcccggcg cgacgataat
     3001 catcgtacgc ggcgcgtacg cggcggcgcc gatggtgatg cgatacgagc cggcggcaag
     3061 cgcgatcggc gccggctcag cagtcccggc gatcgcgccg tcgtaactct cgccagcggc
     3121 cgactcggcg tgataccaac cgttgagcgg ctgcccatcg cgcgcatcga tcacgcggat
     3181 cgccactccc tgcgacggcg acaacttcac ttccagcggc ggcacgccat cctggccgac
     3241 cgtcaccgga accgctgccg caccgtaact ggctttctgc acgcgcgcct cgaagcgccc
     3301 cgccgcgacc tgatcgaacg agaaaccgcc actcgcatcg gacaaggtcg accggacgtc
     3361 agacgcgttc ccatccgtcc gtagcagatc gacacgcgca ttggcgatcg ccgcgtcggt
     3421 cgtggcgtcg ctcacccgtc ccgcgactcg cacaccgcgc agatcgatgt cgaacgtcga
     3481 cgagcccttg acttcgtaga ccgtcgagta cggcccttgc tcggtgtcgt ataccgtcac
     3541 ggcgtagctg ccgttgtcga ttccgctgat ctcgtagcgt ccgtttccgt ccgccgccga
     3601 gcgtgcatag cgcagcgcgg cggattgggg tgcgaacgac acttcgacgc ccggcaacgg
     3661 cctcccgttc cgcgtgatcc ggccgttgat gatgatctcg gcggtgaaat cgaagtcgac
     3721 gttcaccgtc gcgccagcat cgagctgcac gaccttggtc accgcgttgc gcgcactgcc
     3781 cggcagcggt ttgacgctcg cacccacgcg caccgagccg gctgcggcgc cttcgatgcg
     3841 atagtggccg ctctcgtcgg gcgatgccga ggcggtggcg ccgtccgccg accacgcttg
     3901 caccgaggcg ctttgcagtt ccgcccgcgt cagaccggtg atcgctcccg tgatgacgcc
     3961 ccctcgattg atcacgagac gcaacatgct tggagccgga atctccacgt cgcgcagcgt
     4021 ggcagagccg tagcctgcgc gcgtcgcact gatctcgtag tgtccggcag gagcgccggc
     4081 gatgacgaac gtcccacctt catccgtttg cgacgacata cccgcggtgt ccgtcgccga
     4141 gaacgcacgg acgtcggcac ccgcaaccgg cgcgcctccg tcgagcacga ccacgccggc
     4201 gatcgacgag ccggcgccga gctgcgcgtc gacttcagac gaggagccgg aaagcgtgac
     4261 gctcttgcgg acggtcacca gcccgcttcg gctgaacgcg agggtggtcg gtcccgcgtc
     4321 gaggctgtcg agcgcatacg cgccatccgg atcggtcagc gtgaacgaat cgttcgtcgt
     4381 cgcgccgcgc gtcgacgttg tcggatcgac gcgcaccagc accccgccgg caggaccgcc
     4441 gtccggtccc gtcacgtgac cgctcacccg cacaccatga tcgagtgcca cctcgatccc
     4501 ctcgacgctc tttccgctct cgacgttgat gttcgggacg cgagccatca cgtagccggg
     4561 cgcggtgacc gacagcgttt gcgacccaac cggcgcgcca tcgattgcaa acgtgccatc
     4621 gtcgctcgtg aaggcacgca tggcaggcgt tcgcatcaat gccgcgctgc cgcgggtgat
     4681 gccggcatcg aacgacgtga ccggctgact tgtgcctttg tcgatgactc gtccggcaat
     4741 acggccgccg gcgggcactt cgacattgac gtccgcggcc ggcgctgtga cggtgcgtgt
     4801 ggcctgaatg agctccgtcg gtttcttgaa cgcaagcgtc acctggccgg gtgccagatc
     4861 gatgatgcga aatctcccgt cggcactcgt ctgcaccgga ggcgcgtcgc cgccgctgat
     4921 gatgaagatg tcgacgccgt ctaccggcgt ctcgccacac gtgacgcggc cgctgatctc
     4981 gacacccgct tccagcgtca cttcgagcgg cggcgtgctt gcggtcacct gcgtgcgcac
     5041 cgtgcgaggc gcgaagcctg ccgcggtaac ggtgacgtcg tacgtgcctc cgcgcacacg
     5101 caccgcgaac gttccgtctg ccttcgtcct caccccctcc tcggcgtccc gcactccgcc
     5161 ggctcccggc tcggtggccg taaccgcagc acccgacacc ggtttggcgt cgggggcgat
     5221 gacgcggccg gcgagcgcga ccccgcgcgg aagggtgatg acgatgtccg ccgtccgtga
     5281 gcccggtgcg acacggatga ttccggagtg cgccgccggc attcccgtct tcgtcgcgtc
     5341 gagctgaacg ttcccgtctt cggcaattcg cagaatgaag tgaccgtttg gcgcggtgat
     5401 ggcccgcgag gtcatgcgga cggaggatgg cgtccacagc atgtcgccgc cgacacgccg
     5461 ggtgctgatc gagacaccgg cgacggcacg cgcgccctcg tcgatcacgg agcccgatat
     5521 acgcgcgagt gcggctgcgt agagagtctt ccgcaccgtg acaccgggcg gcgcgttgac
     5581 gacgagccgt ggcgtggcgt agcccggatg aaccgcgttc aactcgtatt cgccgccagc
     5641 aagaccggcg attgtgaagt ttcctttgtc gtcggtgacg ctccacgcgc cggggtcgag
     5701 accgccgtaa cgcggcgcag ccgccgtcac ctgcgcgccc gcgatggcga ttccgctctt
     5761 gatgtcgcgg atggatccgc tgattgctgc ggcggacccg aggcggaaga gaaactgcct
     5821 cgcgccaccg atgagccgcg cgctgatccg atcgccggct cgcgccgcga tcctgcgtgc
     5881 gccggcgggc gcatgctcga tcgcgaacgt tccgtccgct ccggttttcg cgaggatcaa
     5941 atcgtcgatc tggatcgacg cgcccgcgac cggcgtctca ccgtcggcag cgacgacgcg
     6001 tccgttcagc acgatgcccg cagtcatggc gacatcggga ttcctcagag cgaccgtgtc
     6061 gatctcgcgc tcgaccggcg cgtagccggt gtgctgcacg aggatccacg acacgagttt
     6121 cttcggatcg ggcagccagt actgcccatg tgcatccgtc gttgccacgc cctcggcgcc
     6181 gccggaacgg acgatgaccg tggcatcagg caatggcttg ccattcgctg tgacggtccc
     6241 acgaacgagc ggctgcgacg gcgcctgcag aacgcctgca tcttcgccgt tggccgaacg
     6301 gacaccaatc gcaggctgtc ccgaaacctc aacgcgaaga tcgaccaccg tagccttcgg
     6361 atcgatggag aaacgcccgt tcgcgtcggt cgcccccgtc acgagcggct tccgcgcagg
     6421 atcggccgaa acccaccgca cgcgctgctc ctccgaagtc tcgagcgcaa acgcggcaac
     6481 ctttgccgac gcaatcgccc gcccatcgcc acccaccacc gtccccgtaa tcgcgcccca
     6541 ggcagcgaac gaagaaacga agatcgaaat cgccaggaac acagccctca cgcgaggcct
     6601 ccgattgcga tgtcattaca tcatgcctga gttgtgcgat gagggaaagc ggacggggct
     6661 gcgacacgat cgagttgtag cgccgtcggc ccgacggctg taccgccggc ggcccgccgg
     6721 ccagtgttcg cgacaagccg gatcggcgta aactgatttc gatgcctcag atcgccatcg
     6781 actacgacaa agaaaaactc cgcgacttct gccgcaagtg gaagatcacg gagttctctc
     6841 ttttcggcag cgtcgtgcgc gaggacttcg gaccggagag cgatgtggat gtcctcgttg
     6901 cattcgcagc cgacgcacat tggtcattgt ttgacatgtc ggatatgaac gatgagctga
     6961 agatcctctt cgaccgcgac gtcgatctgc tgacccgccg cgccgtcgaa ggaagtctga
     7021 atccgatccg caaaacctcg atcctcagct cggcggtgat gctcgatgtt gcgtgagagc
     7081 ggattccttc aagacattct gctgtcggcg cgactcgcta tctcatatct tgagcgaacc
     7141 tcggtggagg aattcgcaaa ggacgttcaa ctgcaggatt ccgtcattcg ccggctcgag
     7201 atcatcggcg aagcagcgag tcatatcgcg gaggaagcag ccactgaatt gccagagctc
     7261 ccgtggcgca agatggtgga catgcgcaac ttcgccattc atcaatactg ggatgtcgac
     7321 gtcaacatcg tttgggaaac agtgagaaat gatttgccag ccgctgtcgc cgccatcgag
     7381 cgctacctcg ctctctgaca gtcatctgac atgaaaacgc ctgagggcga tcctttgtcg
     7441 accttgatgc tcgtcgtcgc gtggtcgagg cgtagcgccg tcggctcgac ggctggaccg
     7501 ccggcggccc gccggcaggt gttcgctaca agcccgatcg gcgtaaactg atttcgatgc
     7561 ctcacatcgc catcgactac gacaaagaaa aactccgcga cttctgccgc aagtggaaga
     7621 ttacggagtt ctctcttttc ggcagcgtcg tgcgcgagga cttcggaccg gagagcgatg
     7681 tggatgtaat ggtgacgttc gaaaagggcg ccccgtggac gctcttcagc ctggtccaca
     7741 tggaagacaa gctggccgag atcttccgca ggccggtcga tctgtgcgaa cgtcccggcg
     7801 tcgagcgaag ccggaatcgc taccggcgcg agtcgatcct ttcgagtgcg gtggcccttg
     7861 agctctaagt tgtttctcac ttggacccgt ctcaaccatt gaagctgtca ggccggatcc
     7921 aaccatcgag gcgggacgcc tcgacggccg cggtaaagac gaccgcactc ctcaatgcac
     7981 gtcggagtgc gtgcgtctcg cacgcgggca ctcgggcgtc ctcgcccgag tgcggtgggg
     8041 gtggaggccg aaatccgtcg acttcttgct tgcgtgaagt gcggcttcga acgctacctc
     8101 gcctctgaca tatcatcccc cccatgcttc ctgaaaaaac attcgaaggc cgagtcggca
     8161 tcatcactgg cggtgggacc gggatcggtt tcgccatcgc gcgcgagctg gcgaagctcg
     8221 gcgccaccgt catcctcgcc agccgcagtg ccgagcacct cgggcctgcc gtggagcagt
     8281 tgcggagcga gacgggacgc gccaacgctg ccgatttcat cgcgctcgat gtccgagaag
     8341 ccgatgcggt cgaggccatg gcggcgaagg ttcatggcga tcatggacac atcgacttcc
     8401 tcgtcaataa cgccgcaggg aacttcatcg cccccgccga tcagctcacc gtcaacggct
     8461 ggaactccgt catcggcatc gtgctcaacg gcacgttcta ctgcacgagc gccgtcggca
     8521 aacggatgat cgcggcgaag aagggcgcga tcctcaatgt catcgccaac tacgcctgga
     8581 ccggcgcccc cggcgtcgtc cactccgcca gcgccaaggc cggtgtcctg gccatgaccc
     8641 agacgctggc cgtcgaatgg gcgcgccaca agatccgcgt caacgccatc gcccccggac
     8701 cggtacacac cgagggcgca tcgaagagac tcttcccgaa cgagatgatc gaggaaggga
     8761 tccgcaaaac gattcccctg cgccgcttcg cgagtctcga agaggtcgcc aacgcggcgt
     8821 cgtatctcct ctccgactac gcctcatacg tcaccggcga agccatggtg atcgacggcg
     8881 gccagtggct gagcggcgcg gattactgga cgcgcgtgca gcagatggcg ccgaagtgac
     8941 cgcgccggct gtcgcctaat gctgagtcga atagcgcggc agtacccatg aattggaacg
     9001 attctggagg ctcaaatgca gggcgttacc tcacctgaat ggtcaacctt tctaggtacg
     9061 gtcaagacgg cgaacgacga attgggtgat cccgaggtgg tctggtatcg aggacacccg
     9121 gacgccgatc attatctctt ggcgtccctt cttcgctacg agaacggcct aacgaaggag
     9181 aaatatctct ttgagagctt tcaaaaattt tccgacagaa tcttcaaaag gcgcatcagt
     9241 gagtgggaga cgctgtttga gatgcaacac tacggcgtac ccactcgact gctagattgg
     9301 actgaaagct ttggagtcgc cctattcttc gctgcctact acagcaagcg gcgcgcatta
     9361 accaatgacg ctgcgatcta cctgctgtct ccccatcgcc taaacaagta cagcggtatc
     9421 aataaggtct acagaatccc cgaagacgaa ggcaacttct cgtacaaggg catctactgg
     9481 aaccgcacac cgttcgccgc gactgcacct atcgcgattg aaccgatttt tattaacgat
     9541 aggatgcttg cccagcgtgg catgttcacg gttcaccatg atgaaatcaa cccgatcgag
     9601 actgagtttc cagccgccat taagaaggtc gtgataccaa acaacgtcgt cgaggcagca
     9661 atggattttc tagatctcac gaatattaat gagtattctg tgtttccaga tcttgcggga
     9721 attgccggct acctcaagag cgagtcggat ctggctccgc gctggccctg agtagcgttt
     9781 agccgagatt tcgatcggcg aaagtcaccc gaagcaggaa gccttacggt ttctttaggt
     9841 agtccaccgc cacatgcgcc atcgcccgca cacccaactc caacccgctt tcatcgacat
     9901 agaacagcgg tgaatgattc gaggcggctt cctcggcggt ctggttcttg ggtcgtgttc
     9961 ccagccagaa gaagagtcca ggcactttct gctggaagaa cgagaagtcc tcggcgccga
    10021 gggtggcgtt gactacttcg acgttggcgg ggccgactat gcggcgcagt gtgggggcca
    10081 tttgttcggt gatggcggga tcgttgtagg tgatggggta gccttcttcg atcgagaccg
    10141 ttgccgttcc gccaccgctc ctggcgatgt cttcggctgt gcgtttgatg cgcgcgtgga
    10201 tctcgtcgcg catcttcgcg tccaatgacc ggattgtgcc gatcatctcg acgtcgtcgg
    10261 ggatgatgtt gttgcggacg ccgccgtgaa tggcgccgac ggtgacgatg gacggggcca
    10321 gtgacgaatc gacctggcgg ctggggatgg tctgcaggcc gaggacgatt tgggaggcga
    10381 tgacgatcgg atcgacgccg agccaggggt aggcgccgtg cgtttgtttg ccgtgcaccg
    10441 tgatcttgaa cgagtcgacg gcggccatca tcgagcccga gcgataggcg atctctccga
    10501 ccgggtagcg cgaggtgacg tgcaatccaa agacggcgtc gaccttcgga ttgtcgagcg
    10561 cgccttcttt caccatcagc ttggcgccgc cttcttcgcc ttccggcgcg ccctcctcgg
    10621 cgggctggaa gagaaacttc acggttcccg ggatctcatc gcgaatgccg gagagcactt
    10681 cggcgacgcc gaggaggatg gccacatgag cgtcgtgtcc gcaggcgtgc atgacgccga
    10741 cttcgtggcc gttgaacgtc gtgcgcacgg tcgacttgaa tggcagatcg acctgctcga
    10801 cgaccggcag cgcgtccatg tccgcgcgca gggtcacgac gcgtcccggc ttgccgccgc
    10861 gcaggattcc gatgactcct gtgtgcgcga tcggcgtctt cacttcgatg ccgagctcac
    10921 gcagacggtc ggcgatgatc ttcgacgtcc gcgtctcgcg gttgccaagc tcgggatgct
    10981 gatggatatc gcggcggcag gcgatgacct tcgcttcgac tttcttcgcg gcatcatcga
    11041 tgcgttgatc aagcgtagtg gcggcgtcga gtgtggcagt catgagaacc tccagtgcga
    11101 ggatggcgag caagcgcatg gcgcattgta gatcgcggtt atcactgaac ccatcaccac
    11161 agaggcacag aggacacaga gaaaaactct gtgatctctg tgtctctgtg gttcacgatt
    11221 ctcaacttac cgctactgcg aaacgatctc cgtcttcagc gcttcggctt tgtgaatcag
    11281 ctctccgaac cattcggcga aatgcgtctc gcctttatcc tccgccagcg ctttggtgcc
    11341 cgaggccatg gcgatgaact gttcgatgaa gccgcgaaga tcgtcggcta cacccatcag
    11401 catcagcgtc gacgattgct cctcgatcgc cttgaaggcc tgcagcgcct gggcgtactc
    11461 gttggagaag taggcgaatg tctcgcgagc ttcatcggac cgctggtcgt catcgctcat
    11521 ggtcttgctc gtcggtgcgg attctaggcc actacgcgcg atcacgagaa cgcattccgg
    11581 aagggcgcgt acaattcaac cgccgtctca cgaaaggatg ttcatgcgcc gctggcgttg
    11641 tctgctcctg ctgtcgattg cctgttcgat ttcttcgctt gcccagacac cggtagtccc
    11701 tcacgccggc gagacgatcg aagtatccat cgtcaacatc gacaccgtcg tcaccgacaa
    11761 acacgggaac cgcgttcgcg gcctcggcaa agacgacttt gaagtctacg agaacggcgc
    11821 gcggcagccg ctcacgaact tcgcggagta tgggagcgaa ggcaaagcgt catccgtcgg
    11881 aaccgttacg gcgccggcag ccgaaggcgc cccacgttcc gcgccacagc cgccgccgtc
    11941 gcagccgcga acgatcatcg ttttcgtgga gcgcttccag ctgccctcct ttcgcagcga
    12001 tccggttttt gccgcgatga agaagctgct tcacgagacg gtccgcaaag gcgaccgcgt
    12061 catggtcgtc acctggaacc gcggcattct ggggatgcgc cagcagttca ccgacgatct
    12121 cgtcaggatc gacaaggcca tcgaaatgat cgccgaccgc acgacgacgc tgaatcacga
    12181 tccgatcgcc gacctcgagc aggagatcga ttccatccag gcgttcgaag aggaaacggc
    12241 ggcgatgaac ggcggcagaa tcgctacgtc aaacatcaca aacgaatccg gcctgagctt
    12301 tttcgtcgag cagatggcgc gcttcaccgc gaaggacgcc tacctcgatg aaaagcgaaa
    12361 agtggagacg atcagcacgc tgatgcgctc cgctggcggc acggaaggga agaaagtgtt
    12421 gctgctggcc acgcaccggc tgtcgatgta tgcgggtgcc gaggcactgt ttgcggcagg
    12481 ggcccctctg ctgcccgcgg acttcaagaa ccaattcgat ggcaagcagc tcgtccgatc
    12541 gctgatcgag gccgcgaatc agaccggttt cacgatctac ccgatttacg ccgagggatt
    12601 ggcgatgaca tcaggtgcca cctcggaact acgagcgcgt cagaacaagc cgatcggtta
    12661 cgagtacctc gttctcaaca acgaaacgcc gatgctcgag tacgtggcga aggagaccgg
    12721 cggttcggcc gcgtggggca gcaacgacgt cacgaaactc attcccaccg tcggcgagga
    12781 tctcgattcg tactactcgc tcggctatcg cgccacgccc ggcaaggcag ccgctcgaaa
    12841 gatcgaagtg aaggtcaagg atccgaagct ggtcgtccgc gcacggacgc agtacatgcc
    12901 gaagtcggac acggcccgga tggacgagcg cgtgatcgcc actcttttcg gaaaccagcc
    12961 gccagcgtcg ttcgaagtga aagtccggct ggcaccgccg aagctcgatg agcagaagcg
    13021 ctacatcatc ccggtcacga tcgaggtgcc gatctccgcg ctgacgacgc tgcccgccgc
    13081 aggcaacgtt cataacggcg ccttcaccgt ctacatggcc tggggcgcag tcctcggcgg
    13141 cctgagcgaa ccgcaccgcg acacgaagca gttctcaatc gccgacgccg atctggcgaa
    13201 ggcgaaaggc aactacttca cgtaccaggt cggtgttgcg tcggccacgc cgtatctgcg
    13261 aatcgcgctc ggcgtgtacg acgaagtgtc gcgtgattac gcgttgaatc tgatcgatct
    13321 ggaaccgccg atgaagaaga agtaatcgcg ctgcggttgg cgacttacgc aacaagaact
    13381 ttgtgtcatc ctgagtcgcc ggagccgcgc ctccacgcta cgttcacaac cggcaggcat
    13441 cgcggcggag gacgatcgaa ggatctcaaa cttttacgga tcgtgctttc ccagcgcgag
    13501 cgaacgtgat caggccacac tcttcgtaac ttgagatcct tcgccgtcct gcgccgcaga
    13561 tcgtgtagcg cgagggatcg agcagcgagg cggctccggc aggctcagga tgacacgaat
    13621 gttgctcagc gctttcttgg gaggtgcgac gaaacagtgg tcatcaatcc agcgaacgca
    13681 ccggcccgag gttcaggttc agatcgtcgt ccgccaggcc gaaaatctgc ttcatctccg
    13741 ccatcttttc ttcgagcttc atcaatgcgg cgccgacgcg ctcgatctct tcatcggtga
    13801 gggtgccggc gtccatgcgg cgcaaggcct gcttttcgag gagacgtcgg atcagctcga
    13861 tgatcgagag aaccagtttg gcgaggccgt tctcgacgat gtcggagtcg gcggcgatcc
    13921 ggtcggggat cgtcgattcg gtgaacgtca gcgacgcgcg gaggcgctct tcgtcattcg
    13981 tgatcatcgg caaacacctc cagcggccag ggacccgaga gaaggaacgg gaccgacgcg
    14041 aagccgcgtt tgagctcggc gccggcgtcg ttcaccgcgg cgacggagtc ggctcgcacg
    14101 agcagcgcga attcgagcga tgcgttgtcg cggttcgacc agcgatgctt caccgccagc
    14161 ggcgtcagat tcttttccac agcatcacga aaaagcggat cgatggatgc ggcctgtgta
    14221 gcgtcgtgaa gtgcgcgaaa gtacgcggcc ccggatgcga agtcgtgacg atcaggacgg
    14281 gcctgcggcg acgccgccgc caccttcaac gtcatctcca ccatccctcg gcactcctgc
    14341 aacgtgcgcc gccaccgctc gaggtgcgcc gcgcacttgg tcatcgcatc gctcgacgca
    14401 tgcgcggtga agccgtagcg gatggcgatg aacgtgccgc ggtcgagcag caacgcgcgc
    14461 gtttgcgcga cgcgaagaag gagcgcgcgg tcgccgaggg gctggtcgtc cgcgacaggc
    14521 atcccgctga gccagagatc gccgaccgcg agcggctggg cgaccggctc gatatcgact
    14581 cgttcgagat gcgcgccgat gacgaccagc ttcatttgat ccgcttcggc tgcgaggtcc
    14641 ggatgatggc gtcgacgttg gcgagcacca cgttgagccc gaggtagaga aggtcgacgt
    14701 cgccgatgga gacgaccgcc tcgccagcaa tcaccacgcc gcggtcgagc aggtgatcga
    14761 gggtctcgag caggctcagt tcgtcgtttt cgtcagacat gtgaagcaga gacaagagac
    14821 aggagacaag agaacagggc aatttctgat ctcttgtctc tgctctcagg ccctgtatct
    14881 ccttcccaac aaggaccaag agtctagaaa ccagagacaa gggatcaggg cgattttctg
    14941 atctcttgtc tctgatctct ggcctctcac tcctccctcc ccacaaacgt atacggcggc
    15001 cacggtccgg tcaacgcaag ggtcacgcct tcgctgccgt agcgctgcga caacgcctcg
    15061 cgaagctcga cgaggcgcga ttcctcgtcg cggttgatga gcacattgat ctgcgggaac
    15121 gcgccggagc gctgctgccg cgtctccgcc acggtgtcgc aggccagctt ctcgagcacg
    15181 gcgctctcga cttcggccat cacctgttgc tctgcctcgc gggaggcacg cttcttctcg
    15241 tcgtcgagtt tcttgcgcag caggaacgcc ttccccgccg cggctccgtc gatctcctcg
    15301 gataacacgc gcagcgaatc gacgcgcctc acgagcgcct cgctccaacg ctccggatcg
    15361 aactccagtc gaagcgtcca ctcctgcttt ccttcgagcc ggtcgagaag cgtcgaaagc
    15421 cgggcgtgct cggcttcgag gtgtgcacga agggtttctg ccgaggcgaa gagcgtgaag
    15481 gcgcgcaacg gaatgatcgt gccgctgtgc atcaacgcgc tcatcacgct ctgatgccga
    15541 tagccgatgc cgccgagcca ctcgacgtcc tttgcgcggg cgtcgatcac ggcctgcgag
    15601 aaatcgacgt cgtcgaccgg cgtaacgaac gccgccaacg ctccggcagt gacctcctgc
    15661 aagcgatccg atccgtcgat cgcctcgagc cgttcgggca gcggacggcc cgcgcggccg
    15721 atcgcataga cgtagagcac ttcaggcatg gggagagtct gccacaacgc aggtcagggc
    15781 gacgttcggc gcacttcgtc cgaatcgatc agggcgcgga cgacgcccac gcgcgtctcg
    15841 cgtttcttga gcgcgctttc gaaatagcgc tgcccgtccg cgtccgccgg ccggtcgaga
    15901 atcgtggcgt aggcgaggcg gatgaagtcg gtatcgttga ggcaggcgga gttgtacgca
    15961 agggtctcgg cgagttcccc gagatcgacc ttgcctcttt gtagtttcgt catcgccgcc
    16021 tgaggatcga agccgagcct gccgaggaga aactggagct ggggcttttc cagcgcccga
    16081 gtcttctggg gaacgcctaa ccacgcggcg ttttcatcga gcagctcggc gggcggctcg
    16141 ttgatgcgga aggcgcgcgt cagctccggg cgcgagtcgg ccagtttcag ggcgctaacg
    16201 gccggcatca ccgcgatcag attgaagcgt ccggcgagcg ggcgcgtgtc tcgcgaattg
    16261 ggatcgaccg atgcaggaat gttcgcacgc gagaaggcga cggtcaggaa atttacgccg
    16321 tcgcgccagc gcgcccgagg gacatcgaac ggatagtcct tccagtcgcg gacgagtgtg
    16381 atctgcgcga gcggagcgcc gttgagtgag atggtcgcga cctgcggcgg tgcgcctcgg
    16441 aaatcgaatg gcgccatgcg aagcagcagc cggtgatcga tcgacgccga cgcaatcagc
    16501 atgaccgaca ccggctcagg gcctgtccaa cgcgagagat cgccgctctc gccctcggga
    16561 ccgtaccagc cgccgccgag gaagaaggcg ttttctgcgc cgaggtgcat gacgtgggag
    16621 gggtagcggg cgacgagcgc gcgcgtgtcc gcggcggtga ggcgattgag cagaaggtgc
    16681 tggatgccgg gcgcgtagtg cagccggaac gattcgagcg ggctggccag cacgaccggg
    16741 aactgacagc tccagtcgcc gatgtcgccc gggcggtgaa agcccgcgct gacgatccag
    16801 agcggcgtgt tgtg
//
LOCUS       DATLVW010000193         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_503691, whole genome shotgun sequence.
ACCESSION   DATLVW010000193 DATLVW010000000
VERSION     DATLVW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503691"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VNN25_06230"
     CDS             <1..774
                     /locus_tag="VNN25_06230"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXH91159.1"
                     /translation="LIALAVIGIVYGALVAWVQPDMKKLVAYSSVSHLGFCVLGIFAL
                     NQTSIEGSILQMVNHGLSTGALFLCVGVIYERRHTRLLADYGGLAKTMPVFATFFIIA
                     MLSSVGLPGLNGFIGEFLILAGSFQTWPRATIVAATGVILAAIYLLWLIQRVFFGPIT
                     KEENKNVREIAWNEIVALVPLVVLMVWIGVRPNVFLRKMTPSVQQLLQIVHKTDASKA
                     LVAGVRSRASGVGDQNRSHEAMLRPPTPDSRLPRPGGVR"
     gene            771..2294
                     /locus_tag="VNN25_06235"
     CDS             771..2294
                     /locus_tag="VNN25_06235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXH91160.1"
                     /translation="MNLTPLLENGWMNAILPELILSIGGMVLILFDAFAPKLRGATAS
                     LAVVVFLVASWGERLVIAGNWFGGTYQISAITIIFDYTFLLAAILATLFARAYLAREG
                     FERGEFYAMLMWGTVGMMMMAKGLDLLIVILGLELLSICIFILVGFHRRIAVSNEASL
                     KYFLMGAFATGFILYGTALFYGATGSTNFAAFLRYFATADATNPLLTIAFVLMISGFG
                     FKLAVAPFHGWAPDVYQGAPSPVAGWLSVAPKAATLIALIRIFASMTPVIQQASWMKM
                     VAALSILSMIIGNAVAIAQRDLKRMLAYSGIAHVGYMLIALLSLRDESVAAISIYVIT
                     YALMNIGAFGVVSMLAKNQNDPQTLDDIAGLGFRRPFYGLALTICMFSLSGLPPTAGF
                     IAKFYIFKTAVDSGHMTVALIGILMSIVSVYYYLRVVYFLYMKEGASAAVNDHAEPHG
                     VHEISQPPLPTPHSPLLDTDIFGTSALLISIVGILAIGLFPTPLFNAAGAAARALLP"
     gene            2305..2955
                     /locus_tag="VNN25_06240"
     CDS             2305..2955
                     /locus_tag="VNN25_06240"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HXH91161.1"
                     /translation="MPNEPTDISELTTRRLSVYLRCLTYLQLAGQETVSSGDLAEQFH
                     LNSAQIRKDLACFGEFGTRGVGYNVPRLKDHLVHELGLDRIRRLLIAGAGNLGMALAD
                     YGGFSEGGFEIAAIIDNDETKIGRKSRSGLEVLAWDDLATIVREHRVDIGVVAVTAEA
                     AQKVYDAFADAGINAVLNFAPTQLRARESVKLKSVDLRINLESLSFYLKNREDGES"
     gene            complement(2971..4893)
                     /locus_tag="VNN25_06245"
     CDS             complement(2971..4893)
                     /locus_tag="VNN25_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91162.1"
                     /translation="MNFGFYETHLERISDNGVIDPKPVIVPPTPGVVVQDGKRNLYFY
                     AMFKTGKLMDVFLQVVDVNGVAGPPQLLFTTLSVSASKSLSAALVGSRLLVSYGEFRA
                     TDVPALNAAFVHVFEVGKDGTLTAVGSPIQLTTKSDNTYQGRFVSRIVCNAQTCAYWL
                     AGDYPSALRVIPTAELASTRPDEGEVVLEVDNAVNQQTQPVIAAGDDGYLAAWNEHDA
                     TDHWQAITARVNADGQTIQRNGLRMPNSKQSELNVAWGNGQWLVAFGNQAIRVDAAGN
                     AVDAQPFPTGVGRTMAVAWSGSSWLVAGVLPNGQLTAVEVRTEGSVGVLQTIFRSADY
                     DPSLVSDCALVWDGSGYFLPFITGYYSSQGGAYGFHDGRVYATRLSDQGVPIQPVPLL
                     LSTKVGGESVVCAVSRGGTTHVAWSTYDGSTSGRQSTDTFILELSATSPLRVNGVPGR
                     IGGGVLQSLTSDGHGGYRLIRSVSDPYAIRYYSYGVLDVLLLTPGVFVYGERQTSIAA
                     ERADVACRDDDCAVVVGALSHDNQNQERAELLFVRELTPTQPPATPQIHVARTGGGVE
                     VWWDRIPDALGYLVEAADAGEGFHVVDATTRDGTGMLVESAAATVRVKAWNYGGLSPA
                     TTMSVPSPPRRRASSR"
BASE COUNT          917 a         1666 c         1417 g          998 t
ORIGIN      
        1 ctcatcgccc tggccgtgat cggcatcgtc tacggcgcac tcgtggcgtg ggtgcagccg
       61 gacatgaaga agcttgtcgc ctactcctcc gtctcgcacc tcggcttctg cgttctcggc
      121 atcttcgctc tcaaccagac atcgatcgaa ggctcgatcc tgcagatggt caaccacggc
      181 ctctccaccg gcgcgctctt cctctgcgtc ggggtgatct acgagcgccg ccacacgcgg
      241 ttgctcgccg actacggcgg gctggcgaag acgatgcctg tcttcgcgac gttcttcatc
      301 atcgccatgc tctcctctgt tggattaccg ggcctgaacg gcttcatcgg cgagttcctg
      361 atcctggccg gctcgttcca gacctggccg cgcgcgacga tcgtggcagc gaccggcgtc
      421 atcctcgccg cgatctacct gctctggctc atccagcgcg tcttcttcgg accgatcacg
      481 aaagaggaga acaagaacgt tcgtgagatc gcctggaacg agatcgttgc tctcgttccg
      541 ctggtcgtgc tcatggtctg gatcggcgtc cgcccgaacg tcttcctccg caagatgacg
      601 ccaagcgtgc agcagttgct tcagatcgtc cacaagaccg acgccagcaa agcgctggtc
      661 gcgggggtcc ggagtcgggc gtcgggcgtc ggagaccaga accggtcgca cgaggcaatg
      721 ctccgacccc ccactcccga ctcccgactc ccccgccccg gaggtgtccg ttgaacctga
      781 ctccgctgct cgaaaacgga tggatgaatg ccatcctgcc cgagctgatc ctctcgatcg
      841 gcggcatggt gctgattctt tttgacgcct tcgcaccgaa attgcgcggc gccaccgcat
      901 ccctggccgt ggtcgttttc ctcgtcgcaa gctggggcga gcgtctcgtc atcgccggca
      961 actggttcgg cggcacgtac cagatcagcg ccatcaccat catcttcgat tacacattcc
     1021 tactcgccgc gatcctggca acgctctttg cgcgcgcgta cctggcgcgc gagggattcg
     1081 agcgcggcga gttctacgcc atgctgatgt ggggcaccgt cggcatgatg atgatggcca
     1141 aggggctcga tctcctcatc gtcattctcg gcctcgagct tctctcgatc tgcatcttca
     1201 tcctggtcgg cttccatcgc cgcattgccg tctcgaacga agcgagcctc aagtacttcc
     1261 tcatgggcgc gtttgcgaca gggttcatcc tgtacgggac ggcactcttc tacggtgcga
     1321 ccggctcgac gaatttcgcc gccttcttgc gctacttcgc cacggccgat gcgacgaatc
     1381 cgctgctgac gatcgccttc gtcctcatga tctccggctt cggattcaaa ctcgccgtcg
     1441 cgcccttcca cggctgggct cctgacgtct atcagggagc gccgtcgcct gttgccggat
     1501 ggctttccgt cgcgccgaaa gcggccacgc tcatcgccct cattcgcatc ttcgcctcga
     1561 tgacacccgt gatccagcag gcctcgtgga tgaagatggt cgccgcgctt tcgatcctgt
     1621 cgatgatcat cggcaacgcc gtcgccatcg cgcagcgcga cctcaagcgc atgctggcct
     1681 actccggcat cgcccacgtc ggctacatgc tcatcgcgct gctctcgttg cgcgacgaat
     1741 cggtggccgc aatcagcatc tacgtcatca cctacgcact gatgaacatc ggcgcgttcg
     1801 gcgtcgtgtc gatgctggcg aagaaccaga acgatccgca aacgctcgac gacatcgccg
     1861 gcctcggctt ccgccgcccg ttctacggtc tggcgctgac gatctgcatg ttctcgctct
     1921 ccggcctccc gccaaccgcc ggtttcatcg ccaagttcta catcttcaag accgccgtcg
     1981 actccggcca catgaccgtc gccctcatcg gcatcctgat gagcatcgtc tcggtctatt
     2041 actacctgcg cgtcgtttac ttcctgtaca tgaaggaagg ggcgtcggcg gccgtcaacg
     2101 atcacgcgga accacacgga gtacacgaga tctcgcaacc cccactcccc actccccact
     2161 ccccactcct cgataccgac atcttcggca ccagcgcact gttgatctcg atcgttggca
     2221 tcctggcgat cggcctcttc ccaactcccc ttttcaacgc ggcaggcgcg gcagcgcgcg
     2281 cacttctgcc gtagccctcg acttatgccg aacgaaccga ccgacatctc cgagttgaca
     2341 acccgccggc tatccgttta cctccgctgt ctgacgtatc ttcagttggc cgggcaggag
     2401 actgtttcgt ccggggacct ggcagagcaa ttccacctca actccgcgca gatccggaaa
     2461 gacctggcct gcttcggcga gttcggcacg cgcggagtcg gctacaacgt gccccgcctc
     2521 aaggaccacc tcgtccatga gctcggcctc gaccgcatcc gccgtctttt gatcgccggt
     2581 gccggcaacc tcggcatggc actggccgac tacggcggct tcagcgaagg tggcttcgag
     2641 attgccgcga tcatcgacaa cgacgagacc aagatcggcc gcaagtcccg aagcggcctc
     2701 gaggtgctgg cgtgggatga cctcgccacc atcgtccgcg aacaccgcgt cgatatcggc
     2761 gtcgttgccg tgactgccga ggccgcgcag aaggtgtacg acgcgttcgc ggatgccgga
     2821 atcaacgcag tcctcaactt cgcaccgacg caactgcgag ccagagagag cgtgaagctg
     2881 aaatcggtcg atctgcgcat taacctcgag tcactctcgt tctatctgaa aaaccgggaa
     2941 gacggcgaga gctaaagcgc gtgcgtaggg ctaccgactg gaagcgcgcc ggcgtggcgg
     3001 agaaggtacg gacatcgtcg tcgccggcga cagtcctccg tagttccacg ctttgactcg
     3061 tacggtggcc gcagcgcttt cgacgagcat gccggtgccg tcgcgcgttg ttgcgtcgac
     3121 cacatggaat ccttccccgg cgtccgcggc ttcgacgaga tatccgagcg cgtccggaat
     3181 ccgatcccac cagacttcga cgccccctcc ggttcgagcg acatggatct gcggcgtcgc
     3241 gggaggctgc gtaggcgtca gttcacgcac aaacaagagc tccgcccgtt cctgattctg
     3301 gttgtcgtgg ctcaaagccc caaccacaac cgcgcaatcg tcatcgcggc aggcaacatc
     3361 tgcacgctcc gccgcaatcg acgtttgtcg ctcgccataa acaaagacac ccggtgtcag
     3421 gagcaagacg tccagcactc cataggagta gtagcggatt gcataaggat ccgacactga
     3481 tcggatgagc cggtacccac cgtgtccgtc tgacgtcaag gactgcagaa ctcctccacc
     3541 gatacgaccc ggcactccgt tgacacgcaa ggggctcgtc gccgagagct cgaggatgaa
     3601 ggtgtcggtg ctctgccggc cggaggtgct cccgtcgtaa gtactccagg ccacgtgcgt
     3661 cgtcccgccg cgcgaaaccg cgcagaccac cgactcccct cccaccttgg tggagagcag
     3721 cagcggtact ggttgaatcg ggacgccctg gtccgaaagg cgtgtggcat agactctgcc
     3781 atcgtggaat ccgtaggcgc cgccctgaga ggagtaatag ccggtgatga acggaaggaa
     3841 gtacccgctt ccgtcccaca caagagcgca atcgctgacg agactgggat cgtaatccgc
     3901 gcttctgaat atggtctgca aaactccgac gctaccctct gtgcgtactt ccacggcagt
     3961 aagttggccg ttcggtagaa ccccggccac caaccatgac gagccgctcc aggccactgc
     4021 catggtcctg cccactcctg tcgggaaggg ctgcgcatcg accgcgtttc ccgcagcatc
     4081 gacgcgaatg gcctgattgc caaaggccac cagccattgg ccattccccc atgccacgtt
     4141 aagctcgctt tgtttagagt tgggcattct cagaccgtta cgctggatgg tctgtccatc
     4201 ggcgtttact cgtgcggtga tcgcctgcca gtggtccgtt gcgtcatgtt cgttccacgc
     4261 cgcgaggtag ccgtcgtccc ctgcggcgat gacgggctgg gtctgctggt tgaccgcgtt
     4321 gtccacttcg agaaccactt ctccttcgtc gggacgcgtg gacgcgagtt cggcggtggg
     4381 gatgacgcgc agcgcgcttg gataatcgcc cgccagccaa tacgcacacg tctgtgcgtt
     4441 gcagacgatt ctcgacacga atcgaccctg ataggtgttg tccgacttgg tggtgagttg
     4501 gatgggcgac cccacggcgg tcagcgtgcc atcctttcca acttcgaaaa cgtggacgaa
     4561 ggcagcattg agcgcaggaa catccgtggc gcggaactcg ccgtacgata cgagaagacg
     4621 cgatcctacc aacgccgcgg acagtgactt ggaagcggag acggaaaggg tcgtgaacag
     4681 gagctgcggt ggtccagcga caccattcac gtcgactacc tgcagaaaca catccatcaa
     4741 cttccccgtc ttgaacatgg cataaaaata gaggttgcgt ttgccatcct gaacgaccac
     4801 gcccggagtt ggcggaacaa tgactggctt gggatcgatc acgccgttgt cgctgatgcg
     4861 ctccaaatga gtctcgtaaa aaccgaaatt caccgcgatg aagccatcgc ttgtagagaa
     4921 aattggcgcg aggtcaccgc tcccgaatgc ttgcgcaaca ggctttccaa gaggatgacc
     4981 atctccgtcg agcatttg
//
LOCUS       DATLVW010000194        11901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_214267, whole genome shotgun sequence.
ACCESSION   DATLVW010000194 DATLVW010000000
VERSION     DATLVW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214267"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VNN25_06250"
     CDS             <1..1209
                     /locus_tag="VNN25_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH91163.1"
                     /translation="NHSPANAPITYSADFSWAHRFGMHCAGCHAAAQEPQFAKPADHG
                     YQAIGDFRVGLHMIPGHDILVGTEARRLDRAEHKAELSPDASVVTGYNKVAIYAQDQF
                     DVVKNLLRVVAGVRYDDKTKLFSAKTSPRLSLVYTPNDRLVVRGGYSTAFRFPTFSEL
                     YQDSNFFTVTTQIGIPPFPLSIFKPNPNLNPEEISTFELGGEYQISPTVSMKADLYRS
                     HLHNFIVVVQRFNPPPATPTLGWENQPADARISGGELELRSNFAQRVTGFVNWSHQSE
                     SQMGTGVDSSGIPFEFVYSPKDKVNLGAYGGPVNGVRGALEVAWRGSYTAPRDWFFIR
                     SEFTSFATKPLPSYALVNARVSYDIPNTRLRATIFGNNLLKKRPEETMVGGVNRLAGR
                     EFFGQMEMRF"
     gene            1892..3853
                     /gene="acs"
                     /locus_tag="VNN25_06255"
     CDS             1892..3853
                     /gene="acs"
                     /locus_tag="VNN25_06255"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXH91164.1"
                     /translation="MANEIDALLQEHRSFPPSDAFRRNANANDEKVYDVKDREAFWAG
                     WAAELDWQTKWDKVLEWEPPYAKWFVGGKLNASANCLDRHLAERGDKQAIIWEGEPGD
                     VRTFTYRELHAEVSKFANALKSLGVTLGDRVAIYMPLVPEAIIAMLACARIGAAHSVV
                     FGGFSSEALRDRIQDAEAKVLITATAGYRRGNLVPLKKLADAATADCPSIEHVVVVMR
                     RAAAEELDDTVSMSPGRDCWYHDLMSKASADCPPTWVDSEQMLYTLYTSGTTGKPKGI
                     IHTTGGYLTGCYATCKWIFDLKENDIYWCTADIGWVTGHSYVVYGPLAAGATVFVYEG
                     APDWPKQDRFWSMIEKHKITIFYTAPTAIRAFMKWGHELPESHDLSSLRLLGSVGEPI
                     NPEAWMWYQKWIGGERCPIVDTWWQTETGAIMISPLPGLVATKPGSATKAFPGIDAKI
                     LNEAGDEVEVGGGYLAITSPWPSMLRGIWRDPERFQAQYWAKWPGIYFTGDGARRDED
                     GFFWLLGRVDDVINVAGHRIGTMEVESALVDHQSVAEAAVVGINHEIKGTAIAAFVIV
                     KSSRLEEAKKDPAKFEKELKDHVSEKIGAIARPEKILVTADLPKTRSGKIMRRLLRDI
                     AEGRTLGDVTTLADPTVVASLKDKYEEES"
     gene            3855..4550
                     /gene="fabG"
                     /locus_tag="VNN25_06260"
     CDS             3855..4550
                     /gene="fabG"
                     /locus_tag="VNN25_06260"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXH91165.1"
                     /translation="MSSALITGGARGIGRALVERLTRDGWNVAFTYVNSTELPEGFAL
                     QGDVRDAARNEAIVAEVTAKFGSIDALVNNAGIRRDALLYNMTDDEWREVLQTNLDGA
                     MSMTKAVLPSMMKQRRGAIVNVASLSGLHGVVGQTNYAAAKGALIALTRSLAREVARS
                     NIRVNCIAPGLVDTDMIATLDAEVKKEMIRAIPMRRAVQPAEVAAAIAFMLSEDASAI
                     TGQVLCVDGGTSA"
     gene            complement(4683..5357)
                     /locus_tag="VNN25_06265"
     CDS             complement(4683..5357)
                     /locus_tag="VNN25_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91166.1"
                     /translation="MIKQIAVAIVFNLAPVALAQTYIVPDGDCGAITLHATRGTGSPI
                     AADRLKDAYVYLPPHRVELNPAAGSGSLDFHANVTEGDKVVMASVDLKPEISGKETRT
                     EHAKAFVFCGPAPLADWQFSAVSGLEIIPQDWNGRRPQLKAGDTMRFIAVDTTTRKIL
                     DVPMDLYRAGAGFVAHGRHDMRSGMDFRYPEPGRYLVTTTYRRPDPQQPALWLVDTST
                     LTFEVK"
     gene            complement(5478..5894)
                     /locus_tag="VNN25_06270"
     CDS             complement(5478..5894)
                     /locus_tag="VNN25_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91167.1"
                     /translation="MAKRLIIAFAAGFVATLVFHQAVIALLHNFGYVPRAAWPMQPVP
                     PFGIPAVISLSFWGGVWGMIMMPIIEGRRGAAFWIAALLFGAIFPTVVAWLVVAPLKH
                     MPIAGGWNPKTMAIGPIVNGAWGLGTALIYRVFIPR"
     gene            complement(5897..6829)
                     /locus_tag="VNN25_06275"
     CDS             complement(5897..6829)
                     /locus_tag="VNN25_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HXH91168.1"
                     /translation="MGRALITGGAGFLGSHLCELFLERGHEVICIDNFITGNPANIQH
                     LIGHEGFSFVKYDVTNYIHIDGPLDYVLHFASPASPIDYLEKPIQTLKVGSLGTHKTL
                     GVAKDKGARYLIASTSEVYGDPLVHPQKEDYWGNVNPVGPRGVYDEAKRFAEAMTMAY
                     HRFHGVQTRIVRIFNTYGPRMRIKDGRVVPAFISQALRNEPLTVFGDGSQTRSFCFVT
                     DLIEGIYRLLMSDEVNPTNIGNPNEMTVLQFAEEIKRLTGSNAPIEFKPLPEDDPKIR
                     RPDITKARTLLGWEPRVPLDEGLTKTIDYFRRVL"
     gene            complement(6829..8154)
                     /locus_tag="VNN25_06280"
     CDS             complement(6829..8154)
                     /locus_tag="VNN25_06280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXH91169.1"
                     /translation="MDIAVIGTGYVGLVTGAGLADFGNDVICCDIDPNKIEALNNGLI
                     PIYEPGLDTIVNRNVNEKRLRFTTDLPEAIRGSRAIFIAVGTPPKPDGSADLRYVEDV
                     ARTIAQHMNGPKLVITKSTVPIGTGRLIERILDESGNGHKGSVVSNPEFLREGSAIED
                     FLKPDRVVIGASDEESIGLMKEIYAPLHSLEIPFVVTNVESSELIKYAANGFLATKIS
                     FINEVAVMCELLGADVQDVARGIGLDSRIGPKFLQAGPGFGGSCFPKDTSAVADIARQ
                     YGYEFEIIEAVLRVNKNIKQRMVGKVVDALGDVRGKTVAILGLAFKPETDDMRDSPTI
                     PLIKGLQGMGAVIRAYDPGAIENSKKIFENVTYCGDAYETADGADALVIATEWNEFRA
                     LKLERIKKLLKQPLIIDLRNVYDPQRMKAEGFTYISVGRNEDVRVREHV"
     gene            complement(8298..9608)
                     /locus_tag="VNN25_06285"
     CDS             complement(8298..9608)
                     /locus_tag="VNN25_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXH91170.1"
                     /translation="MATKPKEKTIRVGLLVAVTFVVLMIFIFLIGSEQKIFSRKNEYK
                     VRLDNVTGLAEGNPVKISGVTVGVIKDITLPYDPKMHDVDIELMVDKKYAERIRTDSR
                     ARLKKLGLLGGDSYIDISAGSPKFDSLEPGSLIPAARQTNVDQLISSGEDLVDNFVQI
                     SYSLKNVLGRVDRGEGLIGELTSTPETKQRITDTFLTTLNKTNAILGHVESGKGLVGK
                     LVYDDAYAAQLTGSLQETAHSLQGLVASVESSLKTGQGTIPALLNDPEGKKKVFDLVD
                     NLRVTSANLAALTASYQNGQGLVPRLMNDKAYGDQALMEFTGLLKQLNDTVAKVNRGE
                     GTAGKLISDPSVYESVNDILIGINESKLLRWLIRNRQQSGIQKRYDTESTKPPAAAPP
                     PDVKPVPSPKSETPVPNVPVVTGTAPATTTSPTTTTTTSTAPPI"
     gene            complement(9753..10535)
                     /locus_tag="VNN25_06290"
     CDS             complement(9753..10535)
                     /locus_tag="VNN25_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91171.1"
                     /translation="MPERRSHARDLFVEFKNVTKSYGTKQVLRGVDLKVYRGEVLVIL
                     GGSGSGKSVTLRHMLGLEAPDSGHVFVEEEDITDHPEEELYRVRKKFGMLFQSGALFD
                     SMTVFENVAFPLREHADMSQEEIARAVREKLELVNLPNTEQLMPVDLSGGMRKRVGLA
                     RSIVLDPKMILYDEPTTGLDPITSQKINELIIDLQSKLNVTSVVVTHDIQSAFSVGDR
                     IAFLNKGIFEWVGTMEEARDADHPVLREFLKASAVTAAQPTH"
     gene            complement(10583..11350)
                     /locus_tag="VNN25_06295"
     CDS             complement(10583..11350)
                     /locus_tag="VNN25_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91172.1"
                     /translation="MHRVRGHIDDALLHLGGITDLAIETIQQFVRKPVDREIVVAQFD
                     QIGVRSMSIVAITSLFIGMVLALQTAYSLSDFGGSLFIGKVVSLSLVRELAPVLMALM
                     VGGRVGAGIAAELGTMKVTEQIDALRALATNPVRKLVVPRVLATTIMMPLLTTLACFI
                     GIIGGLIIAVGSLHLSSNFYIRSVIETVKYNDLASGIGKTFFFGFAIGLIACYNGLST
                     TGGADGVGRSTTATVVTASITVLIMDFFLTKLFLFIF"
     gene            11529..>11901
                     /locus_tag="VNN25_06300"
     CDS             11529..>11901
                     /locus_tag="VNN25_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HXH91173.1"
                     /translation="MKPEDFNLQAGRFVKTLDGATAFVPSPLPPSLQVSWELLDRVTR
                     AERAIANLRGIGATLPNPYLLVSAFVRREAVLSSRIEGTQASVGELIVFEAAGNAAPE
                     HSDVQEVANYVAALDYGLHRLA"
BASE COUNT         2334 a         3504 c         3709 g         2354 t
ORIGIN      
        1 aaccattcgc cggccaacgc gccgatcacg tattcggccg acttctcctg ggcgcaccgc
       61 ttcggcatgc actgcgctgg ttgccacgcc gcggcgcagg aacctcagtt cgccaaacct
      121 gccgatcatg gataccaggc catcggcgat tttcgcgtcg gattgcacat gattccgggc
      181 cacgacatcc tggtcggcac ggaagcaagg cgtctcgacc gcgccgagca caaggccgag
      241 ctttcgcccg acgccagcgt cgtgacgggg tacaacaagg tcgcaatcta cgcgcaggat
      301 cagttcgacg tcgtcaagaa cctgctgcgc gtcgtggccg gagtgcgcta cgacgacaag
      361 acgaaactgt tctccgccaa gacctctcct cgcctctcgc tcgtctacac gccaaacgat
      421 cgtctggtcg tccgcggcgg ctactcgacg gcgtttcgct ttccgacgtt cagcgagctt
      481 taccaggaca gcaatttctt tacggtcact acgcagatcg ggatcccgcc gttcccgctc
      541 tcgatcttca agccgaatcc gaacctcaac ccggaggaga tcagcacctt tgagcttggt
      601 ggcgagtacc agatctcgcc gactgtctcg atgaaagcgg acctctatcg ctcacacctt
      661 cacaatttca tcgtcgtcgt gcagcgattc aaccctccgc cggcgacgcc gactctcgga
      721 tgggagaacc agccggccga cgcacgcatc agcggcggcg agctcgagct gcgatcgaat
      781 ttcgcgcaac gtgtgaccgg cttcgtgaac tggtcgcacc agtccgaatc gcaaatggga
      841 acgggtgtcg attcgagcgg catccccttc gagttcgtct actcgccgaa agacaaggtc
      901 aacctcggcg cctacggagg acctgtcaac ggcgtgcgcg gagcactcga agtcgcctgg
      961 cgtggcagct acaccgcgcc gcgcgactgg ttcttcatcc gatccgaatt caccagcttt
     1021 gctacgaagc cgctgccgtc ctacgcgctc gtcaacgcgc gcgtcagtta cgacatcccg
     1081 aacacgcgcc tccgcgccac gatcttcggc aacaacctgc tgaaaaagcg ccccgaggag
     1141 acgatggtcg gcggcgtgaa ccggttggcg gggcgggagt ttttcgggca gatggagatg
     1201 cggttctaag cccaccccta ccgatgaacc gcgatcataa gttgacgact cgtgcaatct
     1261 tgatcgcgac cacatcctta ctcctgatcg actgtcacag gcgagacact gctggtgcag
     1321 cgggctcgcg ccgttctgtt ccaaaggtcg tccaaactgt gactgagtct gttcgacaac
     1381 cgcggcccga cgagctgtgg cccgttccaa agcgggcctc tagaatcgac gatcacatgt
     1441 gggctttgac gctcgtgccc ggacattgga atctacggcc gaaaagacgg tccagaacac
     1501 aacttgtgta tgagataact tcatacgatg agaccggtaa aattgatagc ggcccttcgg
     1561 tcagaattgt gcaactcgag tacacgccac cacgttggca gcctctgttc ttgcagatgg
     1621 tggttggcga gaccaggcga gtgtggctga ctgacactaa tgaaccccga tccgtctatg
     1681 acgtagagtt gaggaacatc attggtgttg agtgaaaccg gcattaaggg cgagaacgcc
     1741 gacaccggca ccgagcctct cgaccgtgcg ggagaagcgg cccgactgaa agggtgatgc
     1801 tttcgaggtc gtgaggtcac gatcggcggg cgcgccgtcc tctctgcacg cgacttttta
     1861 gaaactctcc ctacgctaga atccgaccac catggccaac gagattgacg ccctgctgca
     1921 ggaacatcgc tcctttccgc cctcggacgc gtttcgccgc aacgcaaatg cgaacgacga
     1981 gaaggtttac gacgttaagg atcgtgaggc gttctgggcc ggctgggcgg cggagctcga
     2041 ctggcagacg aagtgggaca aagtgctgga gtgggagcct ccgtacgcga agtggttcgt
     2101 cggcgggaag ctcaacgcct cggccaactg tctcgaccgg catctcgcgg agcgcggcga
     2161 caagcaggcc atcatctggg aaggggagcc gggcgatgtc cgcacgttca cctatcgcga
     2221 gctgcatgcc gaagtctcga agtttgcgaa tgcgctgaag tcgctcggcg tcaccctcgg
     2281 cgaccgggtc gccatctaca tgccgctcgt gccggaagca atcatcgcga tgctcgcctg
     2341 cgcgcgcatc ggcgcggcgc actccgtcgt gttcggcggc ttctcgtccg aggcgttgcg
     2401 cgatcgcatt caggacgcgg aagcgaaggt tctaatcacg gccactgcgg ggtatcgccg
     2461 cggcaacctc gttccgctga agaagctcgc cgacgccgcc accgccgact gtccgtcgat
     2521 cgagcacgtc gtggtggtga tgcgccgcgc ggcagcggaa gagctggacg acacggtgtc
     2581 gatgagtccc ggacgcgact gctggtacca cgatctgatg agcaaagcct cggcggactg
     2641 cccgccgacg tgggtcgatt ccgaacagat gctctacacg ctctacacga gcggaacgac
     2701 cggcaaaccg aaaggcatca tccataccac cggcggctac ctcaccggct gctacgccac
     2761 gtgtaaatgg attttcgatt tgaaagagaa cgacatctat tggtgcaccg ccgacatcgg
     2821 atgggtgacc ggccactcgt acgtcgtcta cggcccgctc gccgcgggag caacggtatt
     2881 cgtctacgaa ggcgcgcccg attggccgaa acaagaccgt ttctggtcga tgatcgagaa
     2941 acacaaaatc acgatcttct acacggcgcc gactgcaatt cgcgcgttca tgaagtgggg
     3001 acacgagctg cccgaatcgc acgatctctc ttcgcttcgt ctcctcgggt ccgtcggcga
     3061 gccgatcaat cccgaggcgt ggatgtggta ccagaaatgg atcggcggcg agcgttgccc
     3121 gatcgtcgac acatggtggc agaccgagac gggcgcgatc atgatctcgc cgctgcccgg
     3181 tctcgtggcg accaagccgg gctccgcgac caaagcgttt cccggcatcg acgcgaagat
     3241 cctcaatgag gcgggcgatg aggtggaagt cggcggcggc tatctcgcga tcacaagccc
     3301 ctggccatcg atgctgcgcg ggatctggcg cgatccggaa cgtttccagg cgcagtactg
     3361 ggcgaagtgg cctggcatct acttcaccgg cgacggagcg cggcgcgatg aggacggctt
     3421 tttctggctc ctcgggcgcg tcgacgacgt catcaacgta gccggtcacc gcatcggcac
     3481 gatggaggtg gagtcggcgc tggtcgatca ccagtcggtc gccgaggccg cagtggtcgg
     3541 catcaatcac gaaatcaagg ggacggcgat cgcagcgttc gtgatcgtga agtcatcgcg
     3601 gctggaggag gcgaaaaagg atcctgcgaa gttcgagaag gagctgaagg atcacgtctc
     3661 cgagaagatc ggcgcgatcg cacggccgga gaagattctt gtcaccgccg atctgccgaa
     3721 gacacgcagc ggcaagatca tgcgccgcct cctgcgtgat atcgccgaag ggcggacgct
     3781 cggcgatgtg acgacgctcg ccgacccgac ggtggtggcg tcgctgaagg acaagtacga
     3841 ggaggagagc tgagttgtcc tcggctctca tcaccggtgg cgcccgcggt atcggccgcg
     3901 cgctcgtcga acgtctgacg cgcgacggtt ggaacgtcgc tttcacgtac gtcaactcga
     3961 ccgagctgcc cgagggcttc gcgctacagg gcgacgtgcg cgatgccgca cgcaacgaag
     4021 cgatcgtggc cgaggtgacc gcgaagttcg gatcgatcga cgcgctggtg aacaacgccg
     4081 gcattcgccg cgatgcgttg ctttacaaca tgaccgacga tgaatggcgc gaagttctgc
     4141 agacgaacct cgacggcgca atgtcgatga ccaaagcggt gctgccgtcg atgatgaagc
     4201 agcgccgagg tgcgatcgtg aacgtcgcat cgctcagcgg actgcacgga gtcgtgggcc
     4261 agacgaacta cgccgccgca aagggcgcgc tcatcgccct cacacgctcg ctcgcccgcg
     4321 aggtcgcccg cagcaacatc cgcgtgaact gcatcgcccc cggcctcgtc gacaccgaca
     4381 tgatcgcaac gctggatgcc gaggtgaaga aggagatgat ccgcgcaatc ccgatgcgcc
     4441 gcgcagtgca gccggctgaa gttgccgccg caatcgcgtt catgctgagc gaggacgcaa
     4501 gcgcgatcac gggtcaagtg ttgtgcgtgg atggtgggac gtcggcgtag ttatctagcc
     4561 tgttagaatc tcgcatcgtg ggtagattgc tacagaaggc tggtctgacg cagcctttcg
     4621 atccggacaa gatgttggtc tgaccgggtc gtctaactct tcgctgcacc agcctccagg
     4681 cctcacttga cttcgaacgt caacgtgctt gtgtcgacga gccaaagagc tggctgttgt
     4741 ggatcgggac ggcgataggt ggtcgtgacc aggtagcggc ccggttccgg gtatcggaag
     4801 tccataccgc ttcgcatatc gtgcctgccg tgtgcgacaa agccggcgcc cgcgcggtag
     4861 agatccattg gaacgtcgag aattttcctt gtcgtcgtat ccacggcaat gaaccgcatt
     4921 gtgtcgcccg ctttcaactg cggcctgcgc ccattccagt cttgcggaat aatctcgagg
     4981 ccgctgacgg ccgagaattg ccagtctgcc agcggagcag ggccacaaaa gacgaaggcc
     5041 ttggcatgct cggtccgcgt ctctttgccg ctgatctccg gtttgagatc gaccgacgcc
     5101 atgactactt tatcgccctc cgtgacgttc gcgtggaagt cgagcgagcc ggagcccgcg
     5161 gcgggattca gttcgactct atgtgggggc agataaacgt aagcatcctt gaggcgatcg
     5221 gccgcgatcg gggaaccggt gccgcgcgtc gcgtgaagag tgattgcgcc gcaatcgccg
     5281 tccggaacga tgtatgtctg ggcgagcgca acgggcgcga gattgaaaac aatggcaaca
     5341 gcgatctgtt tgatcatttt cgttctgtct ccaagccatt acgacgatcg aaggggccca
     5401 acgtcacaaa ttcaggcttc tgattttgcg acaagatgcg ttcgaatgaa agcggttcac
     5461 gcggggacgt ggagaactta ccgcgggatg aacacgcgat agatcagcgc ggtgcccagt
     5521 ccccaggcgc cgttcacaat cgggccgatt gccatcgtct tcgggttcca gccgccggcg
     5581 atcggcatgt gcttgagtgg ggcgacgacc agccaggcga ccactgtcgg aaagatggcg
     5641 ccgaagagaa gcgcggcgat ccagaacgcg gcgccgcggc ggccctcgat gatcggcatc
     5701 atgatcattc cccacacgcc gccccagaac gagagcgaaa tgacggcggg gatgccgaac
     5761 ggcgggaccg gctgcatcgg ccacgccgcg cgcggcacat agccgaagtt gtggaggagg
     5821 gcaatgacgg cctgatgaaa gacgagcgtg gcgacgaacc cggcggcgaa cgcgatgatc
     5881 agccgtttag ccatgattac agaacccttc gaaagtaatc gattgtcttc gtgagcccct
     5941 catcgagagg cacgcgtggc tcccagccca gaagtgtacg agctttcgtg atgtcaggcc
     6001 gtcgaatttt cggatcatcc tccgggagtg gcttgaattc gatgggggcg ttgcttcccg
     6061 tcagtcgctt gatctcctcg gcaaactgca gcacggtcat ctcgttcgga ttgccgatgt
     6121 tcgtcgggtt gacttcatcc gacatcagca ggcggtagat gccttcgatg agatcggtaa
     6181 cgaagcagaa cgagcgcgtc tgcgatccgt cgccgaagac ggtcaacggc tcgttgcgca
     6241 gcgcctgcga gatgaacgcc ggcacgacgc gtccgtcttt gatgcgcatc cgcggaccgt
     6301 aggtgttgaa gatgcgcacg atgcgcgtct gcacgccatg gaagcggtga tacgccatcg
     6361 tcatcgcctc ggcgaagcgc ttcgcttcgt cgtaaacgcc gcgcggcccg accgggttca
     6421 cattgcccca gtaatcctct ttctgcgggt gcacgagcgg atcgccgtac acctcggacg
     6481 tcgaggcgat gaggtagcgc gcccctttgt ccttggctac gccgagcgtt ttgtgggtgc
     6541 cgagcgagcc gactttcagc gtctggatcg gcttctcgag gtagtcgatc ggactggcgg
     6601 gggaggcgaa gtgcaacacg tagtcgagcg gaccgtcgat gtggatgtag ttcgtgacgt
     6661 cgtacttcac gaacgagaat ccctcgtggc cgatgagatg ctgaatgttg gcggggttgc
     6721 cggtgatgaa gttgtcgatg cagatcactt cgtgcccgcg ctcgagaaag agctcgcaaa
     6781 ggtgtgaccc gaggaaaccg gcgccgccgg tgatgagcgc gcgacccatc aaacgtgctc
     6841 ccggacgcga acgtcttcgt tgcggccgac cgagatgtac gtgaatccct cagccttcat
     6901 ccgctgcgga tcgtagacat tgcgcagatc gatgatcaac ggctgtttga gcagcttctt
     6961 gatccgctcc agcttgagtg cgcggaactc gttccactct gtagcgatta ccagcgcatc
     7021 ggcgccatcg gcggtctcgt aggcgtcgcc gcagtacgtg acgttctcga agatcttctt
     7081 cgaattctcg atcgcgcccg ggtcataggc gcggattaca gcgcccattc cctggaggcc
     7141 cttgatcagc gggatcgtcg gcgagtcgcg catgtcgtcg gtttccggct tgaacgcaag
     7201 cccgaggatg gccacggtct tgccgcgaac gtcgccgagt gcgtcgacga ctttgccgac
     7261 catgcgctgt ttgatgttct tgttgacgcg aagcaccgct tcgatgatct cgaactcgta
     7321 accgtactgc cgggcgatgt cggccacggc cgaagtgtct ttcggaaaac aggagccgcc
     7381 gaaaccgggg ccggcctgga ggaacttcgg tccgatgcgc gagtcgaggc cgataccgcg
     7441 cgcgacgtcc tgtacatcgg cgccgaggag ctcgcacatc acggcgacct cgttgatgaa
     7501 cgagatcttc gtggccagga acccgttcgc ggcgtactta atcaactccg acgattcgac
     7561 attcgtcacg acgaaaggaa tctcgagcga gtgcaacggc gcgtagatct ctttcatcaa
     7621 tccgatcgac tcttcgtcgg acgcaccgat gacgacgcga tccggtttga ggaagtcctc
     7681 gatggctgag ccttcccgca ggaactcggg attcgagacg acggaacctt tgtggccgtt
     7741 gcccgattca tcgaggatgc gctcgatcaa gcggcccgtg ccgatgggga cggtcgattt
     7801 cgtgatgacg agtttcggtc cgttcatgtg ctgcgcgatc gtgcgggcaa catcctcgac
     7861 gtaccgaaga tcggcggagc cgtcgggttt cggcggcgtg ccgacggcga tgaagatggc
     7921 gcggctgcct cggatcgctt cggggagatc ggtcgtgaag cgaagacgct tctcgttgac
     7981 gttgcggtta acgatcgtgt cgaggcctgg ctcatagatc ggaatcaggc cgttgttgag
     8041 cgcttcgatc ttgttcgggt cgatgtcaca gcagatgacg tcgttgccaa agtcggccag
     8101 accggctccg gttacgagcc cgacatagcc cgttccgatg actgcaatat ccaactggga
     8161 gtcctccgga aaatagtgcg cgaacgcgcg gcgcgagatc gtatcacgca gagaacggac
     8221 caaacgtagg tcggactctc tagtccgaca gcggtcgaaa tgagtcggac tggagagtcc
     8281 gacctacgtt gagtctctca aattggcgga gctgtcgaag ttgtcgtcgt tgtggttggg
     8341 ctcgttgtcg tcgcaggcgc ggtgccggtg acgacaggaa cgttcggcac cggcgtttca
     8401 ctcttcggcg acggaaccgg tttgacgtcc ggcggcggtg cggccgcagg cggtttcgtc
     8461 gactccgtgt cgtagcgttt ctggatgccg ctctgttgcc ggttgcgaat cagccagcga
     8521 aggagtttcg attcgttgat gccgatgagg atgtcgttca ccgactcgta cacggacgga
     8581 tcgctgatca acttgccggc agtcccttcg ccccgattca ctttcgccac cgtgtcattg
     8641 agctgtttca gcaagcccgt gaactccatg agtgcctgat cgccatacgc tttgtcgttc
     8701 atcaggcgcg gcacgagtcc ctggccattc tggtaggacg cggtgagcgc tgcgagattc
     8761 gcggacgtga cgcgaagatt gtcgacgaga tcgaagactt tcttcttccc ttccgggtcg
     8821 ttgagaagag cgggaatggt tccctgaccg gtcttcagac ttgattccac gctggcgacg
     8881 agaccctgca gcgagtgcgc ggtctcctgc agcgaaccag tgagctgggc ggcgtaggcg
     8941 tcgtcgtaca caagcttgcc gaccagcccc ttgccgctct cgacatgccc aaggatggcg
     9001 ttcgtcttgt tcagagtggt gagaaaggtg tcggtgatgc gttgcttcgt ctccggcgtc
     9061 gaagtcagct cgccgatcag cccctctccg cgatcgacgc gcccgaggac gtttttcagc
     9121 gagtaggaga tctgaacaaa gttgtcgacg aggtcttcgc ccgaggaaat gagctgatcg
     9181 acgtttgtct gccgcgccgc gggaatcagg gagcccggct ccagggaatc gaacttcgga
     9241 ctgcctgcgg aaatatcgat gtagctgtcg cctccgagga gcccgagctt cttcagccga
     9301 gcccgggagt cggtgcggat acgctcggcg tacttcttgt cgaccatcaa ttcgatgtcg
     9361 acgtcgtgca tcttcggatc gtagggaagg gtgatgtctt tgatgacgcc gacagtgacg
     9421 cccgagatct tgaccgggtt tccctcggcc aggccagtca cgttgtcgag gcggactttg
     9481 tactcgttct tgcgcgagaa gatcttctgt tccgagccga tcagaaagat gaagatcatc
     9541 agcacgacga acgtgactgc gaccagaagc cccactcgga tcgttttttc tttcggtttg
     9601 gttgccatcg tttcgtaccg aacggttttc cttgtttgaa gtcagagatc agagatcaga
     9661 gatcagagat cagagacaag agtaagcccc tgatctcttg tctctagtct ctgatctctt
     9721 gtctctgctt tctggtccat atcgcacatc tattagtgag ttggctgcgc ggccgtgacc
     9781 gctgatgctt tcaggaactc gcgcaggacg ggatgatcgg cgtcacgtgc ctcttccatc
     9841 gtgccgaccc attcgaaaat acctttgttg aggaaggcga tgcgatcgcc gacggagaag
     9901 gcggattgga tgtcgtgcgt cacgacgacc gaggtgacgt tcagcttcga ctggagatcg
     9961 atgatcagct cattgatctt ttgcgaagtg atcgggtcga gtccggtggt cggctcatcg
    10021 tagagaatca tcttcggatc gagaacgatc gagcgcgcca ggccgacacg tttgcgcatg
    10081 ccgcccgaca gatcgaccgg catgagctgc tcggtgttcg gtagattgac cagctcgagc
    10141 ttctcgcgta cggcgcgcgc gatctcctcc tgcgacatgt cggcatgctc tcgcagcgga
    10201 aacgccacgt tctcgaacac ggtcatcgag tcgaagagcg cgccgctctg aaacagcatc
    10261 ccgaacttct tgcgcacccg gtagagctct tcttccggat ggtcggtgat gtcttcctct
    10321 tcgacgaaga cgtggccgga gtcgggcgct tcgagtccga gcatgtgccg caaggtaacg
    10381 gacttgccgc tcccggatcc gccgaggatg acgaggactt cgccgcgata aaccttgagg
    10441 tcgaccccgc gcagaacctg cttcgtgcca tacgacttcg tgacgttctt gaattcgacg
    10501 aagaggtccc gcgcatggct tcgccgttcc ggcatagccc gcgcctcggc gcgacggtcg
    10561 ggaatcatcg atgtttcagt catcagaaga tgaacaggaa aagcttcgtg agaaaaaagt
    10621 ccatgatcag gacggtgatc gaagcggtga cgacggttgc cgtcgtggaa cgtcccacgc
    10681 cgtcggcgcc gcccgtcgtg ctcagcccgt tgtagcaggc gatgagtccg atcgcgaatc
    10741 cgaaaaagaa ggtcttgccg atgccggacg cgagatcgtt gtacttcacc gtttcgatga
    10801 cgctgcgaat gtagaagttc gagctgagat gaaggcttcc caccgcgatg atgaggccgc
    10861 cgatgatgcc aatgaagcag gccagcgtgg tcaggagcgg catcatgatc gtggtcgcca
    10921 ggacgcgcgg aacgaccagt ttgcgcacgg gattcgtggc cagcgcgcgc agcgcatcga
    10981 tctgctcggt cactttcatc gtgccgagct ccgcggcgat gccggcaccg acgcggccac
    11041 caaccatgag cgccatcaaa accggcgcga gctcgcgcac gagcgagagc gaaacgactt
    11101 tgccgatgaa cagcgatcct ccgaagtcgg agagcgaata cgccgtctgc agtgccagca
    11161 ccatcccgat gaagagtgac gtgatggcca cgatcgacat cgaccggacg ccgatctggt
    11221 cgaactgggc gaccacgatt tcccggtcca ccggcttgcg gacgaactgt tgaatggtct
    11281 caatggccag gtccgtgatg ccgccgaggt ggagcagcgc atcgtcgatg tgacccctga
    11341 cgcggtgcac cagaccatta gcctgcgggg ccgctgccgg ttcggccggg gcgggggcgg
    11401 ttgtgctttg aaagattgag gtcacggcaa gtaatgaatg ctactaaagg ttaatcgatt
    11461 ggcgccgagt tatactttag cggtcgaaag tataactact ctggagtagt tgaacttaac
    11521 tacacatcat gaaaccggaa gactttaatc tccaagccgg ccgctttgtc aaaacccttg
    11581 acggggcgac tgcattcgtg ccgagtccgc ttccgccctc cctgcaagtc tcatgggagc
    11641 tactagaccg cgttactcgc gccgaacgag cgatcgcaaa tctgcgtggt atcggtgcca
    11701 ctctaccgaa tccgtacctt cttgtctcgg cattcgtccg acgcgaggcg gtgctgtcga
    11761 gccgaatcga gggaacgcag gcgtcggttg gtgagctgat cgtgttcgag gcagcgggaa
    11821 acgcggcccc ggagcatagc gatgttcagg aagttgccaa ttacgtagcg gcactggact
    11881 atggtcttca tcgcctggcc g
//
LOCUS       DATLVW010000195         4411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_7177, whole genome shotgun sequence.
ACCESSION   DATLVW010000195 DATLVW010000000
VERSION     DATLVW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7177"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..2267
                     /gene="metG"
                     /locus_tag="VNN25_06305"
     CDS             198..2267
                     /gene="metG"
                     /locus_tag="VNN25_06305"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXH91174.1"
                     /translation="MAKFYITTAIHYVNDDPHIGHMYENVVADVIARHRRRMGDDVWF
                     LTGTDEHGQKVERAAAKEGILPIELADRVVAKHRELWHKLDISNDDFIRTTENRHRVG
                     VLELIRRIQERTPDDIYLGEHSGWYCQNEETFVPDNQVRDGKDEMGHPVEKTTERNFF
                     FKLARYTQPLLDHYRNNPGFIYPPSRLNEVVSFVEQGLRDLSISRTAITWGIPFPGHP
                     DHVIYVWMDALTNYISALGFGSADHDRFDHDWPADLQLIGKDIIRFHAVYWPAFLMAA
                     GLPLPKRIVGHGWWLRDNQKISKSLGNVVRPYNIIDEFGADAFRYFLLCEMVFGQDQN
                     YSDEAFLGRYNADLANDLGNTLSRAIKMTDTYFGGKTPPTACPSNELLRAAEQIVPEY
                     LKSMDDLSFQRALDAAWKLLVAVNGYIVSKEPWKKFKETGADETLSRILWNTLEAQRI
                     VWVMMAPFMPSTTREALSRLGSDPDAIGVDALKWGGLPAGAPVRAAEAIFPRIDIEAY
                     VGAARAGGGGNVSEKNDQEVKVAGEAVAAPPAASPAAAPPTVPAPASATSAAADASAP
                     HAGAPIETTQAAPPDMAKIGIDQFMEIDLRVADVRAAERVPKSKKLMKMTVFTGDEER
                     TIVAGIAAKYAPEELVGRKIVIVANLQPAKLMGVESNGMVLAASIDGEPSLLSVDASV
                     PAGAKVK"
     gene            complement(2825..2992)
                     /locus_tag="VNN25_06310"
     CDS             complement(2825..2992)
                     /locus_tag="VNN25_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HXH91175.1"
                     /translation="MSTHRPPFTIGYVPNGYSRITYPNGQTPMFSYDHQGRMTNVTNN
                     DPQGNLLASFA"
     gene            complement(3655..>4411)
                     /locus_tag="VNN25_06315"
     CDS             complement(3655..>4411)
                     /locus_tag="VNN25_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HXH91176.1"
                     /translation="TVGERNTSGGVSTDYLFGPGIDEPLAKHTANGAISYYGVDGLGS
                     IVLTTDSSGIMTGSNKYSAWGAPATINGDLFGYTGRESGGPSWFYRARYYDTTTGRFF
                     SEDPLRFAMGPNFYAYVHNNPTILKDPFGLGDLVPFPGNPKPTPPFPNCWPEVREFDE
                     PPFDQRPTGIGDVGRGGARAAVTIALAAINIVVWNCVVAESYCASQSDYCDCMKYANP
                     SGYCTDIFGGCRKPRRPQNSPPPGTCGTCPISQ"
BASE COUNT          939 a         1362 c         1291 g          819 t
ORIGIN      
        1 tttcaatgtt tcgcagctcg atgtcttggt ggcgccggga ttcgtttgga aatggaatcg
       61 agctgatgac aaacctcttc agtccgcgcg cggcgcgctc gtttgcccag acgaccacga
      121 gcaggcgggg ctagccgagc gcctcggcgc ttagtctctc cggatccgct ctcccgcgct
      181 acaatcccgc cccttcgatg gctaagttct acatcactac cgcgattcac tacgtcaacg
      241 acgatccgca catcgggcat atgtacgaga acgtcgtcgc cgacgtcatt gcccggcacc
      301 ggcgccggat gggcgacgac gtctggttcc tcaccggcac cgacgagcat gggcagaagg
      361 tcgagcgagc cgcggcgaag gaagggattc ttccgatcga gctggccgac cgcgtcgtgg
      421 ccaagcatcg cgagctgtgg cacaagctcg acatcagcaa cgacgatttc atccgcacga
      481 ccgagaaccg ccaccgcgtt ggagtcctcg aactgatccg ccgcatccag gagcgtacgc
      541 cggacgacat ctacctcggc gagcattccg gctggtactg ccagaacgaa gagacgttcg
      601 ttcccgacaa tcaggtacga gacggcaaag acgagatggg ccatccggtc gagaagacca
      661 ccgagcgcaa cttcttcttc aagctcgcgc gttacacgca gccgctgctc gatcattacc
      721 gcaacaaccc cggcttcatc tacccgccct cgcggttgaa tgaggtggtc tcgttcgtcg
      781 agcaaggtct gcgcgatctc tccatttcgc gcacggcgat cacatggggc attccgttcc
      841 ccggccatcc cgaccacgtc atctatgtct ggatggatgc gctcacgaac tacatctccg
      901 cgctcggatt cggcagcgcg gaccacgacc ggttcgatca cgactggccg gccgacctgc
      961 agctcatcgg caaggacatc atccgcttcc acgccgtcta ctggcccgcg ttcctgatgg
     1021 cggccggatt gccgttgccc aagcgcatcg tcggtcacgg ctggtggctg cgcgacaacc
     1081 agaagatctc caaatcactc ggcaacgtcg tgcgcccgta caacatcatc gacgagttcg
     1141 gcgcggacgc gtttcgctac ttcctgctgt gcgagatggt cttcggacag gatcagaatt
     1201 actccgacga agccttcctc ggccgctaca acgccgacct cgccaacgac ctcggcaaca
     1261 cgctttcgcg cgcgatcaag atgaccgaca cgtacttcgg cggcaagact ccgccgaccg
     1321 cttgtccgtc caacgagctt ctgcgcgctg cggaacagat cgtgccggag tacctgaagt
     1381 cgatggacga tctctcgttc cagcgcgcgc tcgacgccgc ctggaaacta ctcgtcgccg
     1441 tcaacggcta catcgtcagc aaagaaccgt ggaaaaagtt caaagagacc ggcgcggatg
     1501 aaacgctctc acgcatcctc tggaacacgc tggaagccca gcgcatcgtc tgggtaatga
     1561 tggcgccatt catgccgtcg accacgcgcg aggcattgtc gcgactcggc agcgatcccg
     1621 acgccatcgg cgtcgatgcc ctgaagtggg gcggcctccc ggccggcgcg ccggtccgcg
     1681 cagccgaggc aatctttcca agaatcgata tcgaagcgta tgtcggtgca gcgcgagcag
     1741 gaggaggggg gaacgtgtca gaaaagaacg atcaggaagt gaaagtcgcg ggcgaggccg
     1801 tcgccgcacc acctgctgct tcgccagccg ctgctccgcc aaccgttcca gcgccggcca
     1861 gcgcgacgtc ggccgccgcc gatgcctcgg cgcctcacgc cggagcgccg atcgagacca
     1921 cgcaggcagc gccgccggac atggcgaaga tcggcatcga tcaattcatg gagatcgatc
     1981 tacgcgtcgc cgacgttcgc gccgccgagc gcgtgccgaa atcgaagaag ttgatgaaga
     2041 tgacggtctt caccggcgat gaagagcgca cgatcgtcgc cgggatcgcc gcgaaatacg
     2101 cccccgagga actggtcgga cgaaagatcg tcatcgtggc caacctccag cccgcgaaac
     2161 tgatgggcgt cgaatcaaac ggcatggtcc tcgccgcatc catcgacggc gaaccgtccc
     2221 tgctcagcgt cgacgcaagc gttccggcgg gagcgaaggt gaagtagggc cggagaaggc
     2281 tggaacgctg gccgcctcag acagtctgag atcctcggat gtaggtctaa tgttgttgct
     2341 gctcgtgtgg tgtcgcgtgg gtggacaccc ttgcccggta ctgctcccat ggaaccgcag
     2401 atccacgact gattcgccaa aggccaaagc tccaccaccc cgaaaagggc gcacgttcag
     2461 agccgactaa gacttttgtt actgtcgttt cgatgataat cggcctggtc cgatcccaga
     2521 tcttccatgt gccttccacg agccagaacg ttccgagatc acgggagatg tgaaatcgaa
     2581 acgtatggtc cggtcgcaga atcagcgttt tttgctcata catcgcgccg tagcgatacg
     2641 tgccgggtat tgaggtcgca cacgccgtcc agtgcagcgc aggcgaacgc ggctgaaagg
     2701 gaatgcctgg aaaggtgtgc taattctgcg catggtcggc cgccgggtct aacgtgacat
     2761 ctggttcaaa acaatcacgc aataaatcgt gccggttccg gccacccatt cggtgatcat
     2821 attcctaggc gaacgacgcc agaaggttcc cctgcggatc gttgttcgtg acgttcgtca
     2881 tccggccttg atgatcgtat gaaaacatcg gcgtctggcc attcgggtaa gtgatcctcg
     2941 agtacccgtt ggggacgtag cctatcgtaa atggcgggcg gtgggtggac atgcctcaag
     3001 cgctagtgcg cgatttgcgc cgggcggtcg tggatgctga agccacgcat acgtcacaga
     3061 taacagcgcc aatagcctga atgaacgcgt cgcgatcggc cctgccgcaa aacgagcaca
     3121 caattgtctc gtccgacgtt tttaaggcct gggtcgacgc cgataagaga acatgggcga
     3181 gatatgacac ttgcgtcggt gtgagcgcag cgtcgccaag tacgcgacgc agacggagac
     3241 tgctgtcgtc atgaccaaca acgctctccg caactgcggc gagtttggcc gcaatatcgt
     3301 cgcagcgagt ccgacacgca ggctttcttg tcaagtcaac cagccacgag aaccaccacc
     3361 ggccccgctc acgtagcgca gcctcacata tctcaataca ctcatcacag atcgtcgcta
     3421 acggtgctcg cacgacaaca cgcacgttat gtgcgttctt attacaaaaa gaacagcgga
     3481 cttcgtcggt tggcgcttcc gaagtatccc gttcccgtaa aagtgcttcg aacaaagtcg
     3541 cgagttgggg gagacgcggg tcgcgagcga acgaatgacg caggcggtgc agacaagaga
     3601 cgccagtgtt ctccatcatg gagagttctt caaaagccga acgaagagtc gaggtcattg
     3661 cgatatcgga catgtaccac atgttcccgg tggaggcgag ttttgcggtc ggcgcggctt
     3721 acgacaaccg ccgaagatat ccgtgcagta accgcttgga tttgcatact tcatgcaatc
     3781 gcagtaatca gactgcgatg cacagtacga ctccgccaca acacaattcc agacgactat
     3841 attgatcgca gcaagtgcga tcgtcacggc ggcgcgcgct cctccccgac ccacatcgcc
     3901 gatcccagta ggtcgttgat cgaacggtgg ctcatcaaac tcgcgaacct ccggccaaca
     3961 gttcgggaac ggcggtgttg gcttcgggtt gcccgggaac ggcacaagat cgcctaagcc
     4021 gaaaggatct ttgaggatgg tcggattatt gtggacgtac gcgtagaagt taggacccat
     4081 cgcgaaccgc aacggatcct cactgaagaa ccgccccgtc gtcgtatcgt agtaacgcgc
     4141 gcggtagaac cacgaggggc cgccactttc gcggccggta tagccgaaga ggtctccgtt
     4201 aattgttgct ggagctcccc aagcagagta cttattcgag ccggtcatga ttcccgaaga
     4261 atcggtcgtg aggacgatcg agccgaggcc gtcgacgccg tagtaagaga tcgcgccgtt
     4321 cgccgtgtgt ttggccagcg gctcgtcgat cccaggaccg aacaggtaat cggtcgacac
     4381 gcccccgctt gtgttacgtt cgccgacggt g
//
LOCUS       DATLVW010000196        11350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_135610, whole genome shotgun sequence.
ACCESSION   DATLVW010000196 DATLVW010000000
VERSION     DATLVW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135610"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VNN25_06320"
     CDS             <1..300
                     /locus_tag="VNN25_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91177.1"
                     /translation="YIAPLLLSGHFPASVPITPPWSAATLTGHTSVNRLTFDEAIIFA
                     NHPPAFPESEWASFNLGEFLFGPGSALSLLPILLIILGGGIVIARLATKAAEAPS"
     gene            complement(236..2632)
                     /locus_tag="VNN25_06325"
     CDS             complement(236..2632)
                     /locus_tag="VNN25_06325"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH91178.1"
                     /translation="MFNASWIYVGALYAIAILCARRWVPAVRDGLPWRIAMFFYALVL
                     IYLFRPMTGQYVNLPVDFVNILPPWGDWGHRPTLSNSEMNDLPLQIVPWAHQVREAWL
                     SLHFPVWNPLAGCGYPLLANGQSSAMSPLRLLALPLPLGYAMTAEAAMKLLIALTFMY
                     LLCRRRYDELPSALGAIAFAFCTFVSTWLHFPMVTVAVWLPAAFLALELLIERRTYPR
                     FVFAAVVWATMLYGGHPETVTHVTFFGGLFALWIAFVERPVSPRDAMRSLALAAAAIF
                     VAALLAAPFLTTFAETVKKSQRFQELQIRANGEAPFSNFPSAIATFEPNFYGHAPAER
                     PWGPATAESITGFAGILGIASFFALLFRAIAQRRFRSREVFLLILALIATGIVFNWPV
                     ISALFHFAFRLAANARLRMFLCWIAAAMTAAAIDSARRESSTPFLLGVLATAGLVLYV
                     LLKTSFPSAAMQQTAMIAVFPSIVVLLLSALFVLQARARHIATMLVGFALIVELWNAS
                     DGWNPVLPVDAMYPKTPLIERLERLQAATPATDPFRIVGAGAALFPNTNAMYGFADIR
                     THDPMANGRYLGVLRLLADLETNNYFANWQNAKTPLLNYLNVRYLVGARNVDPGDHAR
                     YRLVYEGRDGRIFENLDVKPRFFAAHDIVLEFRHNMFAQTLAGQDLAKQTVVNILPVD
                     SDRMRQDLLHPTSDPQVKIVEAHDPDFRLRIHSARHALIVSSQPFWPGMRVSLNGNSI
                     RPLAVNGAFLGFTIPPGDWDVRVGYFPATFYEGLASAVTTALLLLSWPIVRSRFLHRG
                     "
     gene            complement(2547..4817)
                     /locus_tag="VNN25_06330"
     CDS             complement(2547..4817)
                     /locus_tag="VNN25_06330"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH91179.1"
                     /translation="MNPTWLYVGTLYAIAVALARRGGNPIPRRIAALFYALVLIFFFK
                     AMTGPYVSVATDIPRLIAPWSASAPGLTKYTVLNMETHDVSMQLAPWAHQVREAWKSG
                     HAPLWNALAGCGYPLLANAQSAALSPLRLLALPLPMVYSMTAEAAMKVLVALTFTFLY
                     CRRRYDALPSCIAAIAFGFGPFVIAWLHFAQGTAACFLPAVLYQIDLLVERRTDARFA
                     MTAVIGACVVFCGHPETAIHILLFAIAYVFWLVVIEKQREFLRALFGAGVVAALLASP
                     LIVPFGENVRDSTRFESQKAWGRDMPFSDRVSLALLVQPRLYGTRPGNPWGPAGAEAV
                     TGFAGILGIASLIAISCETIVRRRFRDREAFFALAALGVLALLANIGPAVTVLHAIIG
                     LAANARLRFLFAWILAVLIAAALHRLQRGTVVPFAIGLAASAALLGAIVMTTPFPSDA
                     ARHDGLISVIPSVIVLIVAMVMVLAPRTRNIGSLLLVAAVIAEMWPLTIGWNSAFPKS
                     TFYPRTPLIEAVLRNHHPGFDRVAGIGGVLFPNTNAMFGIEDVRVHDPMEPARYVHLL
                     GTAITTDYYKKWIDASSPLLDRLNARWVMTEPGHALTDTSRYQLRYSGSDGMLYENLH
                     SQPRFFAAGTRVQIISASSDAYVLSIDTNTPTLINSSVGWSRWWRADAGNGRLATQIV
                     DGAFIGFAVPPGHSRVRVRYVPVSFDIAVVVALLTLLFLICFIIRARVQRFLDLRRRA
                     LRDRDLVRAPLGPGRS"
     gene            complement(4828..5631)
                     /locus_tag="VNN25_06335"
     CDS             complement(4828..5631)
                     /locus_tag="VNN25_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HXH91180.1"
                     /translation="MHPARRTLIAGLGLIGGSIGIALRRRGWHVTYIDPGVSLDDAVR
                     AGAADARSDSFDGPFDVTVLATPVDAAIAAIDRCRSLTTSVCSVLQPLRAAADVHRLD
                     FVAGHPLAGSHERGLAAARADMFEGASWFVDREVAVVQELIAGCGAHAELVTAAEHDR
                     AVALTSHLPQILSTALAAHLHSQNIDPRFAGSGLRTFLRLAGSAASVWSPIIAANREN
                     IAPHAEAVAALVRKMIEQGDEEPFAQANEWNSDVPAAGPAASRRRPDND"
     gene            complement(5631..6185)
                     /locus_tag="VNN25_06340"
     CDS             complement(5631..6185)
                     /locus_tag="VNN25_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91181.1"
                     /translation="MSRTSRLLAITALVVLVSLALAGCSKEKKNESNEPESFAFTIYP
                     GSRYLAQLTDLWKQARKVMRPGDDIPPIAIYDTDAPLDKVAEFYAQSYGYNKVAPDAT
                     NNLSSAKPPAYYRTGDLATDVSGIVPIAQKLNIKLDPSKAVGAYRAAEIDPKTNRPRV
                     TIQRPYFDASTSQTVDRTLIMMTR"
     gene            complement(6303..8009)
                     /locus_tag="VNN25_06345"
     CDS             complement(6303..8009)
                     /locus_tag="VNN25_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXH91182.1"
                     /translation="MDRPRWFLAVAALAALPLIGATSSSSKKAAPDAAPAPVAKGMAM
                     LSAKALAAHDQFLSSDLLEGRGPGTRGDEIAEQYIAAQFESYGLQPGGDNGTYYQNVP
                     LLGIATDRDKSALTFTKNGTAIGGPLKFGDQIVGQNQTQTESETIDSDLVFVGHGVVA
                     PEYKWDDYKGLDTRGKTLVMLVDDPPANAQEPDLFKGKARTYYGRWTYKYEIGTSKNA
                     AGVILIHTTPAAGYGWNVVSNSWGGENSFMKNKPGAPALQFAGWMTEQVARDLFKAAG
                     KDLDALTQAASSRDFQPVPLGVKLSGNVASKLRSFNTTNVVAKLTGSDPKLRDEAILY
                     SAHHDHLGIGPADKSGDTIYNGALDNASGCAILLEMARVWSRMPAPKRSVYFAAVAAE
                     EQGLLGSAYLGQNPPLPSGHIALGMNYDMIPELGRVRDVNMNGIERTTFYPTAQRITK
                     ALGLTIIGDPEPEQGHYYRSDHFSFGKVGIPAFSIDPGHDVVGKGAAFRAAWSDEFRA
                     NHYHQPSDQFDPKWDWSEAVQMAQLGIWMGWDAANAATMPNWKEGDEFRAARDRSLAA
                     AK"
     gene            8201..8419
                     /locus_tag="VNN25_06350"
     CDS             8201..8419
                     /locus_tag="VNN25_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91183.1"
                     /translation="MFTQISDPFHDLRMPLQERVVDKLADMLFASDMILDMLQDPRSR
                     ESAGFTTVEASVRGVYEQINELMKQLTD"
     gene            complement(8485..8733)
                     /locus_tag="VNN25_06355"
     CDS             complement(8485..8733)
                     /locus_tag="VNN25_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXH91184.1"
                     /translation="MLKVRILFFAVLREIAGRDEDLLQLPDGTRAGDVWQRLRAEHTA
                     LRDYDPPPLIAVNENYVGPEEILSDGDELVFIPPVAGG"
     gene            8766..8972
                     /locus_tag="VNN25_06360"
     CDS             8766..8972
                     /locus_tag="VNN25_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HXH91185.1"
                     /translation="MVHEGFTAVMTVDAALALHPAARWVFAAYQIGGCNGCSSAAEET
                     LAEVAQGYKLDLDRLLADLNGLFA"
     gene            9495..10493
                     /gene="selD"
                     /locus_tag="VNN25_06365"
     CDS             9495..10493
                     /gene="selD"
                     /locus_tag="VNN25_06365"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXH91186.1"
                     /translation="MGQDDLRSVLAKLPSHEGLGDLVVGYGTNDDAAVVRIAGDQAIA
                     QTVDFFTPIVDDPYIFGAIAAANAVSDIYAMGATPILGLAIAAFPTDVLPLEVLQEIL
                     RGGSEKAAEAGFPIAGGHTIIDDVPKYGLAVTGIVSVSKLVRNSTAKAGDAIVLTKPI
                     GNGILISAYRALSARRFRRPAVPKLDDAIQWMMMLNRDAAGAMIDVGVNAATDVTGYG
                     LIGHLLEMCDGSGTGAEIRASAVPVLPNARELLARGFRPAGTVRNVETFRHRVDVQAP
                     ESEFTLMCDAQTSGGLLLAVPSEKLASFDARMRQSGLFYATIGTMTEQSGKVTLIP"
     gene            10525..10962
                     /locus_tag="VNN25_06370"
     CDS             10525..10962
                     /locus_tag="VNN25_06370"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXH91187.1"
                     /translation="MPYCPTCKQAWPEGQKECPKDHTPLVDELPYQSVQSDPDGTTWV
                     EIASTGVDDRARIIKGFLDAEGIPAQIENVKFSMEPINFGTMGDIRIYVRSEDETRAQ
                     ELLRQREADFDRLEDDADTIVTDDGTAEVTEDEEIATEPAEKE"
     gene            10959..>11350
                     /locus_tag="VNN25_06375"
     CDS             10959..>11350
                     /locus_tag="VNN25_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91188.1"
                     /translation="MTEEPHAPAPGEREGEHHDSSRRDETPLMPSWVPALIGVVLVSM
                     AALAVYTGIRYRNPPLVNPVIKRRPVRPMTAGGGPPGEPEPGASLIFPGDADNTPAAR
                     EAVTGNARAEITGTGHGITSTVRIWARRG"
BASE COUNT         2231 a         3287 c         3802 g         2030 t
ORIGIN      
        1 tacattgcgc cgttgctgtt gtccggccat ttccctgcat ccgtgccgat tacgccgccg
       61 tggtccgcag ccacgttgac cgggcacacg tcggtcaaca ggctgacatt cgacgaggcg
      121 atcatcttcg cgaatcaccc acccgcgttc ccggaatcgg aatgggcgag cttcaacctc
      181 ggcgagtttc tctttggtcc gggcagtgcg ctttcgctcc tcccgattct gctgatcatc
      241 ctcggtggag gaatcgtgat cgcacgattg gccacgaaag cagcagaagc gccgtcgtga
      301 ccgcgctggc gagtccctcg tagaacgttg ccgggaaata gccaacgcga acgtcccagt
      361 cgccgggcgg aatcgtgaag ccgaggaatg cgccgttgac ggccaggggt cgaatggagt
      421 tgccgttcag cgaaacgcgc attcccggcc agaagggctg gctgctgacg atgagtgcgt
      481 gccgcgcgct gtggatgcgg agccggaaat cgggatcgtg cgcttcgacg atcttcacct
      541 gcggatcgct ggtcggatgc agcaggtcct ggcgcatgcg gtcgctgtcg accgggagaa
      601 tgttgacgac cgtctgcttc gcaaggtcct gcccggcgag cgtctgcgcg aacatgttgt
      661 ggcggaactc gaggacgatg tcgtgcgcgg cgaagaagcg cggcttcaca tcgaggttct
      721 cgaagatccg gccgtcgcgg ccctcgtaca cgaggcgata gcgcgcgtga tcaccggggt
      781 cgacgttgcg cgcgccgacg agatagcgca cgttcagata gttcagcagt ggcgtcttcg
      841 cgttttgcca gttggcaaaa tagttgttcg tctcgaggtc ggcgagcaga cgcagtacgc
      901 cgagatagcg gccattggcc atcggatcgt gcgtgcggat gtcagcgaat ccgtacattg
      961 cattggtgtt gggaaagagc gccgcgccgg ccccaacgat gcgaaaggga tcagtggccg
     1021 gagtggccgc ctgcagacgc tcgagccgtt caatgagcgg agtttttggg tacatcgcat
     1081 cgaccggcag gacgggattc cagccgtcgg acgcgttcca tagttccacg atcagagcga
     1141 atccgaccag catcgtcgcg atgtgccggg cgcgagcctg cagcacgaaa agcgccgaaa
     1201 gcaggagcac gacgatgctg ggaaacaccg cgatcatcgc cgtttgctgc atcgccgcac
     1261 tcgggaagga cgtcttcagc aggacgtaca aaacgaggcc tgcggttgca agtacgccga
     1321 ggaggaaggg cgtggagctc tcgcgccgtg cggagtcgat cgccgccgcg gtcatggctg
     1381 ccgcgatcca gcagagaaac atacgcaatc gagcgttcgc cgccagacgg aacgcgaagt
     1441 ggaatagcgc gctgatgacg ggccagttga agacgattcc ggtggcgatg agcgccagga
     1501 tgaggagaaa tacctcgcgg cttcgaaaac ggcgttgtgc gatggcgcgg aagagaagcg
     1561 cgaagaagga ggcgataccg aggatgccgg cgaatcccgt gatcgactcg gctgtcgccg
     1621 gtccccaagg tctctccgcc ggcgcgtggc cgtagaagtt cggctcgaac gtggcgatgg
     1681 cggagggaaa gttggagaag ggtgcctctc cgttcgcgcg gatctgcaat tcctgaaagc
     1741 gctgggactt cttcaccgtt tcggcgaacg tggtgaggaa cggcgcggca agaagtgcgg
     1801 ccacgaaaat cgccgctgct gcgagggcca gcgagcgcat ggcatcgcgt ggcgaaaccg
     1861 gccgctcgac gaacgcgatc caaagcgcga agaggcctcc gaaaaaggtc acgtgcgtca
     1921 ccgtctccgg atggccgccg taaagcatgg tggcccagac caccgccgcg aagacgaagc
     1981 gcggataggt gcgccgttcg atgagaagtt ccaacgcgag aaacgcagcc gggagccaga
     2041 ccgcgactgt aaccatcggg aaatgcagcc acgtgctgac aaacgtacag aaagcgaagg
     2101 cgatggctcc gagggccgag ggcagctcgt cgtaccgccg ccggcacaag aggtacatga
     2161 acgtcagcgc gatgaggagc ttcatcgccg cttcggcggt cattgcgtat ccaagaggaa
     2221 gcggcagtgc cagcagacgg agcggagaca tcgccgagct ttgtccgttg gcaaggagcg
     2281 gatacccgca gccggcgagt gggttccaga ccggaaagtg cagcgaaagc cacgcttcgc
     2341 gcacctgatg cgcccacggg acgatctgca acggcaagtc gttcatttcc gagttggaca
     2401 gagtgggacg atggccccaa tctccccacg gcggcaggat gttgacgaag tcgacgggaa
     2461 ggttgacgta ctgcccggtc atcggccgga agagatagat caggacgagc gcgtagaaga
     2521 acatcgcgat gcgccacggc aagccgtcac gaacggccgg gacccagcgg cgcgcgcaca
     2581 agatcgcgat cgcgtagagc gcgccgacgt aaatccagga agcgttgaac acgggcgcgg
     2641 atgataaagc agatgaggaa gaggagagtg agaagtgcga cgactaccgc gatatcgaac
     2701 gacacaggaa cgtaccgcac gcggaccctc gaatggccgg gcggaacggc gaaaccgatg
     2761 aatgcgccgt cgacgatctg cgtcgctagc cggccgttgc cagcgtccgc gcgccaccat
     2821 cgcgaccagc cgacgctgct gttgatcaga gtgggtgtgt tcgtatcaat gctgagcaca
     2881 taggcgtcgc tgctcgccga gatgatctgc acgcgcgtac cggcggcgaa gaagcgcggc
     2941 tgcgagtgaa gattctcgta gagcatgccg tcgcttccgg agtagcgaag ctggtagcgc
     3001 gaggtgtcgg tgagcgcgtg gcccggctcg gtcatcaccc agcgggcgtt caatcgatcg
     3061 agcagcggcg aggacgcgtc gatccatttc ttgtagtagt cggtggtgat cgcggtgccg
     3121 aggaggtgaa cgtagcgtgc gggctccatc ggatcgtgaa cgcggacgtc ctcgatcccg
     3181 aacatcgcgt tagtgttcgg gaagagcaca ccgccgatgc cggcaacgcg gtcgaagcca
     3241 ggatggtgat tgcgcagcac cgcttcgatg agcggcgtgc gcggatagaa cgtcgatttc
     3301 gggaaggccg agttccatcc gatggtcagc ggccacattt cggcgatcac ggccgcgacg
     3361 agcagcagag atccgatgtt tcgtgtgcgc ggcgcgagaa ccatgaccat ggcgacgatg
     3421 aggacgatca cgctcgggat cacggagatg agtccgtcgt ggcgcgcggc atcgctcgga
     3481 aagggcgttg tcatcacgat ggccccgagt aacgccgccg aggcagcgag gccgatcgcg
     3541 aacggcacga ccgttcctcg ctgcaggcga tgcaaagccg cagcgatgag gaccgcgaga
     3601 atccatgcga agaggaatcg caaccgcgcg ttcgctgcga gcccgatgat tgcgtgaaga
     3661 acggtcaccg ccggaccgat gttggccaga agcgcgagaa cgccgagcgc ggccagagcg
     3721 aagaacgctt cgcggtcgcg aaaccgccgc cggacgatcg tctcgcagga gatcgcgatg
     3781 agagacgcaa tgccgagaat gcctgcgaac ccggttaccg cctctgcgcc cgccggtccc
     3841 cacggattgc ccggccgcgt cccataaagc cgcggttgca cgagcagcgc aagcgacacg
     3901 cgatccgaga acggcatgtc gcggccccac gccttttgtg attcgaatcg cgtggagtcg
     3961 cgcacgttct ctccgaaagg aacgatgagc ggcgatgcca gcagcgcggc cacgacgccg
     4021 gcgccgaaga gtgcacggag aaattcgcgc tgcttctcaa tcacgacgag ccagaaaacg
     4081 tacgcaattg cgaagagaag aatgtggatg gcggtctcgg gatggccgca gaaaacgacg
     4141 cacgctccaa tcaccgcggt catcgcgaaa cgggcatcgg tgcgccgctc gaccagaaga
     4201 tcgatctgat agagcaccgc aggcaggaaa caggccgctg tgccttgcgc gaagtgcagc
     4261 caggcgatga cgaaaggacc aaaaccaaaa gcaattgcag cgatgcagct cggaagagcg
     4321 tcgtagcgtc gcctgcaata gagaaacgtg aacgtcagcg cgacgagaac cttcatcgcc
     4381 gcctcggcgg tcatcgaata caccatcggc agcggaagag cgagtaaacg caatggtgag
     4441 agcgcagcac tctgggcgtt tgccagcaat gggtaaccgc agccggccag agcattccag
     4501 agcggtgcgt ggcccgactt ccacgcttcg cggacctgat gcgcccacgg tgcgagctgc
     4561 atcgaaacgt cgtgcgtctc catgttgagc acggtgtatt tcgtgagtcc cggagccgat
     4621 gcggaccagg gagcgatgag acgtgggatg tcggtagcga cggagacata cgggccggtc
     4681 atcgctttga aaaagaagat gaggacgagc gcgtagaaga gcgcggcgat gcgcctgggg
     4741 attgggttac cgccgcggcg cgcaagcgcg accgcgatgg cgtaaagcgt gccgacgtag
     4801 agccatgtgg ggttcaacgg ggagatgtta gtcgttgtcg gggcggcggc gagacgccgc
     4861 cgggccagcc gccgggacgt cagagttcca ttcgttcgct tgcgcgaacg gctcctcgtc
     4921 gccttgctcg atcatttttc gcaccagtgc cgcgaccgcc tcggcgtggg gggcgatgtt
     4981 ctcgcgattg gcggcgatga tcggtgacca gacggaggcc gcgcttccgg cgagtcggag
     5041 gaaggttcga aggccggatc ctgcgaaacg tggatcgatg ttttggctgt gcaggtgagc
     5101 ggcaagggcg gtggagagga tctgcggaag gtgactggtc agggcgactg cgcggtcgtg
     5161 ctctgcagcg gtgacgagct ctgcatgcgc tccgcaacca gcgatgagct cctgaacaac
     5221 ggctacttcg cgatcgacga accatgaggc gccttcgaac atgtctgcgc gggcggctgc
     5281 aagtccgcgc tcatgcgatc cggcgagcgg atgcccggca acgaagtcga ggcgatgtac
     5341 atccgctgca gcacggagtg gctgcagcac gctgcagacc gaggtcgtca gggagcggca
     5401 gcgatcgatg gcagcgatag cggcatcgac cggagttgcc agcacggtga cgtcgaacgg
     5461 gccatcgaac gaatcgctgc gcgcatcggc cgcaccggcg cggaccgcgt catcgagcga
     5521 cacgccggga tcgatgtagg tgacgtgcca tccgcgccgg cgcagcgcga tcccgatcga
     5581 tcctccgatg aggccgagtc cggcgatcag cgtgcgccgc gcggggtgca tcaacgcgtc
     5641 atcatgatga gcgtgcgatc gaccgtctgc gacgttgagg cgtcgaaata cggccgctgg
     5701 atcgtcacgc gcggacggtt cgtcttcgga tcgatctccg cggcgcggta cgcgccgacg
     5761 gccttcgagg ggtcgagctt gatgttcagc ttctgcgcga tcggtacgat gcccgagacg
     5821 tccgtggcca gatcgccagt gcggtagtac gcaggaggct tggcggagct gaggttgttc
     5881 gtcgcatcgg gcgcgacctt gttgtagccg tacgactgcg cgtagaactc agccactttg
     5941 tcgagcggtg cgtcggtgtc gtagatcgcg atcggcggga tgtcgtcgcc gggcctcatg
     6001 actttgcgcg cctgcttcca gagatcggtc aactgggcca ggtagcgcga accggggtag
     6061 atcgtgaagg cgaaggattc cggctcgttc gactcgttct tcttctcttt cgagcagccg
     6121 gcgagtgcga gcgagacaag cacgacgagc gccgtaatcg ctagtaagcg tgaagttctg
     6181 gacatttctg tgactccaat gagatgagag ttgatcagtg atcggtggtc agaggtgcag
     6241 catcagcgcc gatcgcgggt tccaatcgac tttagcatac ggacttagag agcaaatcgt
     6301 cgctacttcg ccgcggccag gctgcggtcg cgtgccgcgc gaaactcgtc cccttctttc
     6361 cagttcggca tcgtggcggc gttcgcggca tcccatccca tccagattcc gagctgggcc
     6421 atctgcaccg cttccgacca atcccacttc ggatcgaact ggtcgctggg ctggtggtag
     6481 tgattggcgc ggaattcatc cgaccacgcc gcacggaagg ctgcaccttt gccgacgacg
     6541 tcatggccag gatcgatgga gaaggccggg atgccgacct tgccgaaact gaagtgatcc
     6601 gagcggtagt aatgcccctg ctccggttcg gggtcaccga tgatggtgag gccgagcgct
     6661 ttcgtgatgc gctgcgcggt tggatagaaa gtcgtccgtt cgatgccgtt catgttgacg
     6721 tcgcggaccc ggccgagctc cgggatcatg tcgtagttca tgccgagggc gatgtggccg
     6781 gatggaagag ggggattctg gccgaggtaa gccgatccga gcaggccctg ttcttcggcg
     6841 gcgacggcag cgaagtagac cgagcgtttc ggcgccggca tgcgcgacca gacgcgcgcc
     6901 atctccagca ggatggcgca gccggaggcg ttgtcgagcg cgccgttgta gatcgtgtcg
     6961 ccgctcttgt cggcgggtcc gatgccgagg tggtcgtggt gggcggagta gagaatcgct
     7021 tcatcgcgca gcttcggatc gctccccgtc agtttggcca ctacgttcgt ggtgttgaat
     7081 gagcggagct tgctggcgac gttgccggag agcttcacgc cgagcggcac cggctggaag
     7141 tcgcgcgatg aggcagcctg tgtcaacgcg tccagatctt tgccagcggc tttgaagaga
     7201 tcgcgcgcca cctgctcggt catccagccg gcgaattgca gcgccggagc gcccggcttg
     7261 ttcttcatga acgagttttc gccgccccat gaattggaga cgacattcca gccgtagccg
     7321 gcggcaggag tggtgtgaat caggatgacg cccgcggcgt ttttcgaagt cccgatctcg
     7381 tacttgtagg tccaacggcc gtagtacgtg cgtgcttttc ctttgaagag atcgggctcc
     7441 tgcgcgttcg cgggcgggtc atcgacgagc atcacgagcg tcttgccgcg tgtgtcgagg
     7501 cctttgtaat cgtcccattt gtattcggga gcgacgacac cgtggccgac gaagacgaga
     7561 tcggagtcga tggtttcgct ttcggtctgc gtctgattct gaccgacgat ctggtcgccg
     7621 aacttgagcg ggccgccgat cgctgtgccg ttcttcgtga aggtcagcgc ggatttgtcg
     7681 cgatcggtgg cgataccgag gagcgggaca ttctggtagt aggtgccgtt gtcgccgccc
     7741 ggctgcaggc cgtaggactc gaattgcgcc gcgatgtact gctccgcgat ctcgtcgccg
     7801 cgcgttccgg gcccgcggcc ttcgagcaga tcgctggaca ggaattgatc gtgagcggcc
     7861 agcgccttcg cgctcagcat cgccataccc ttggccaccg gagccggagc ggcgtccgga
     7921 gccgcctttt tcgaactgct gctcgttgcg ccgatgaggg ggagagctgc caacgctgca
     7981 acggcgagga accaacgcgg tcgatccatg tctgatctcc tggttttgat tgaggtggta
     8041 cgccgctgtc cggatggacg cggcaagttg gtaacgatat cgcagacggc agggggtcga
     8101 agtgactccc attggtgact tgtactcgaa aattgagaat gttacgaaaa agagggtagg
     8161 gacatcggta gataaatcac tcctagacag agtgcgagaa atgttcaccc aaatctccga
     8221 ccctttccac gatcttagga tgccgttgca ggagcgcgtc gtcgacaagc tggcggatat
     8281 gctcttcgcc agtgacatga tcctcgacat gcttcaggac ccgcgctccc gcgagtccgc
     8341 cgggttcacg accgtcgagg cctcggtgcg aggcgtctac gagcagatca acgagttgat
     8401 gaagcaactg acggattgat ctgcggttgt cgtcgcccgg tctgaaaagg gagatcgtgc
     8461 cgaagaagac accgaaccgt aacttcaacc tccggctacc ggcgggatga agaccaactc
     8521 gtctccgtca ctgaggatct cttccggtcc cacgtaattc tcgttgacgg cgatcagcgg
     8581 aggggggtcg tagtcgcgca gcgctgtgtg ctcggcgcgc aggcgctgcc agacatcccc
     8641 ggcacgcgtc ccgtcaggaa gttgcaggag atcctcgtct ctgccggcga tctcgcgcag
     8701 gacggcaaag aagaggattc ggactttcag catcgtccgg cattctaact gcgtagaatt
     8761 gggtgatggt gcatgaaggg ttcacagccg tgatgaccgt cgacgccgcc ctggccctcc
     8821 atccagcagc gcgatgggta ttcgctgcct accagatcgg agggtgcaac ggctgttcgt
     8881 cggcggcgga ggagaccctg gctgaggttg cgcagggcta caagcttgat ctcgaccggc
     8941 ttcttgccga tctcaacggg ctttttgcct agaacttgcc atcgaccgtg ggaaaatttt
     9001 gcgcgcgcgt atggggcaag tgcaagtttt tcctcactgc tttcgggaag tgatcaggat
     9061 gacggcggat aggaccgttg ccataactat cgtcttttct attgcttgta gtcggcacac
     9121 ggaattactg gctcgtgatc aagttccatg aaccgctatt tatttgtcct ggcaggggcc
     9181 gtgcagaaag caggagcacg atgaaacagg tttcgacaga ttcgagaccg ccacgcgggt
     9241 cccatcctga ttgcccgcgt ctagtggccg ctgcggaccc cctccttgac tattggaccg
     9301 attttggacg attaatcacc tagcttcctt gaatcccacc aactggtcgc agcggtccag
     9361 cccaacgatg taccacgacg gagccgaaag gctccgttag tgcgtttggg ggataccatt
     9421 catccgtgaa gcggaaaatc aagtcgtacc cgccgctatc gtcgttcgct acgaccggtg
     9481 gctgagcctg caagctgggt caggatgacc tgcgctccgt gctcgcgaaa ctcccttccc
     9541 atgaagggct gggggatctt gtcgtcggtt acggcacgaa tgacgatgcg gccgtcgtgc
     9601 gcatcgccgg cgatcaggca atcgcccaga ccgtcgattt cttcacgccg atcgtcgacg
     9661 atccttacat cttcggcgcg atcgcggcgg caaatgcggt ctccgacatc tacgcgatgg
     9721 gcgctacgcc gatcttggga ctggccatcg ccgcatttcc cacggatgtc cttccgctcg
     9781 aggtgctgca ggagatcctt cgcggcggat cggagaaggc cgcggaagct ggcttcccga
     9841 tcgccggagg ccacacgatc atcgacgacg tgccgaagta cggtctcgcc gtcaccggca
     9901 tcgtcagcgt gtcgaagctc gttcgcaatt cgacagcgaa ggccggcgac gcgattgttc
     9961 tgacgaagcc gatcggcaac gggattctga tcagcgccta tcgtgcactg agcgctcgcc
    10021 gattccgaag gccggccgtg cccaagctcg atgacgcgat tcagtggatg atgatgctca
    10081 accgcgacgc cgccggcgcg atgatcgatg tcggcgtcaa cgctgccacg gacgttaccg
    10141 gctacgggct catcgggcac ctgctcgaaa tgtgcgatgg ctccggtacc ggtgcggaga
    10201 ttcgcgccag cgcggtgcct gtcctgccga atgcgcgcga gttgctggcc cgcggtttcc
    10261 gtccggccgg gacggttcgc aacgtcgaga cgttccggca tcgcgtcgat gtccaggctc
    10321 cggagtcaga attcacgctg atgtgtgacg cgcagacgag cggaggattg ctgttggcgg
    10381 taccgtcgga gaagctcgct tcgttcgacg cgcgcatgcg tcaaagcggc ctgttctatg
    10441 caacgattgg aacgatgacg gaacagagcg gtaaggtcac gctcattccc tgaaatactg
    10501 ccgagacgat tgaggagaga acagatgccc tactgtccaa cgtgtaaaca agcctggccg
    10561 gaaggtcaga aggagtgccc caaagaccac acacctcttg tcgacgagct gccgtaccag
    10621 tcggttcaaa gtgatcccga tggtacgacg tgggtcgaga tcgccagcac gggagtggac
    10681 gaccgggcgc gcatcatcaa ggggtttctc gatgcggaag ggatcccggc ccagatcgag
    10741 aacgtgaagt tcagcatgga gccgatcaac ttcggcacga tgggcgacat ccgcatctac
    10801 gtccgatccg aagacgaaac gcgcgcgcag gagctgctcc gccagcgcga ggccgacttc
    10861 gaccgactgg aagatgacgc cgatacgatc gttacggatg acggcaccgc cgaggtcacc
    10921 gaggacgaag agatcgcaac ggagccagcg gagaaagaat gaccgaagag ccgcacgcgc
    10981 cggcgccggg tgagcgcgag ggcgagcatc atgattcgtc cagacgggac gagactcctc
    11041 tgatgccgtc gtgggtgccg gctctcatcg gtgtggtact ggtttcgatg gccgctcttg
    11101 cggtctacac cggcattcgt tatcgcaacc ctccgctggt gaatcctgtg atcaaacgtc
    11161 gtcccgtgcg cccgatgacg gctggcggag ggccgccggg tgagcccgag ccgggcgcat
    11221 cgctgatctt tcccggcgat gccgacaaca caccggcagc tcgtgaggcg gtgactggca
    11281 acgctcgcgc ggagatcacc ggaacaggcc acggcatcac gtctacggtg cgcatctggg
    11341 cacgccgcgg
//
LOCUS       DATLVW010000197        16346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_52715, whole genome shotgun sequence.
ACCESSION   DATLVW010000197 DATLVW010000000
VERSION     DATLVW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16346
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52715"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VNN25_06380"
     CDS             <1..411
                     /locus_tag="VNN25_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXH91189.1"
                     /translation="IRSLLATFSSEERIRDGSMVFREGESGDKLYIVLDGRVRISKFI
                     PGVGEEALAVLDRGDFFGEMALIDDKVRSADAKAHDGDATVLSIDRATLNEILSMDPH
                     ASLQFLNLLCRMISRRLREINEKIVQWKYMSGGF"
     gene            471..1745
                     /gene="purD"
                     /locus_tag="VNN25_06385"
     CDS             471..1745
                     /gene="purD"
                     /locus_tag="VNN25_06385"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXH91190.1"
                     /translation="MRVLIVGQGAREHALAWKLRQSPLVREVYAAPGNAGIAEIADCV
                     NIGVADIIELADFAEKLKMDLTIVGPELPLTLGIVDEFQKRGMAIFGPTRLAAELEGS
                     KAFSKEFMRKYGIPTAEARVCSSADEAKAALKEFRYPAVIKVDGLASGKGVVIVNSKA
                     EGEEYLKLVFEEKKFGNAANRILVEEFLTGEEVSFMALTDGKKFVSLAPAKDYKKAFD
                     GETGPNTGGMGAHSPAVVLSGEAAAEIVKTIIMPTIQGMAEEGRPYTGCLYAGVMLTP
                     NGPKVLEYNCRFGDPETQVQMLRLEDDLADLCLKVAQGDLGDTKLTWKKEAAACVVVA
                     VDGYPDEFPKGQEVSIDAIDDPNVTIFHAGTIPRNGKMINVAGRVASVCANAPTLSEA
                     LAKAYEAAPKVRFEGARFRKDIGYRALEQRKG"
     gene            1848..2600
                     /locus_tag="VNN25_06390"
     CDS             1848..2600
                     /locus_tag="VNN25_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXH91191.1"
                     /translation="MATSHYRIQNHQPAQTLDLYEGRDPRDVPAYSFREVALSIGVPT
                     STLRAWFVGQSNFDQVLSLPSDWSENQSLSFFNLIEANVVAELRREHGVAMKRVRTAL
                     EFLSDNIPVAHPLVEKELLVTPNQRVYIVHDGRRIDISAGGQWPLDVVVRNLLRRVHR
                     GQRGILTFFPRVTPPSHSVVLEEDYAPIVVDPEVCFGRPLITGTAIPADVVADRYLGG
                     DSIPKMAEDYDLSEEQIEAALRFERLLEADAA"
     gene            2497..2997
                     /locus_tag="VNN25_06395"
     CDS             2497..2997
                     /locus_tag="VNN25_06395"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91192.1"
                     /translation="MATASRRWRRITISRKSKSKPRSDSSDYSKLTQLDLPGFTYFFD
                     STNMVRELADALHQRGRLVHMHNEVFPDRTPDRVWILDVAKRGWIILTRDKRLQTRHL
                     EWIAFLRAKARVLWFKGDRASNVEITEGFLAALPKIDRLALSLPPPYIIKITSSGHVE
                     QIYPEK"
     gene            complement(3023..3502)
                     /locus_tag="VNN25_06400"
     CDS             complement(3023..3502)
                     /locus_tag="VNN25_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXH91193.1"
                     /translation="MKKTAMFLIALASVTPLFAAEHAAAQPIPDQTITVTAKTLEGLP
                     RVKAEAKEHENPAATYEGVTLGAILEHAGVPRGEKLRGPGLRAIVIITATDGYQVVFT
                     VAETDPAFNDRVIILADKKDGKPLPEKEGPFRIVVPGEKRPARWIRNVKTIAVKTVG"
     gene            complement(3601..5862)
                     /locus_tag="VNN25_06405"
     CDS             complement(3601..5862)
                     /locus_tag="VNN25_06405"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXH91194.1"
                     /translation="MADNDRDDALEYHSGGRPGKTEVVPTKPTATARDLALAYTPGVA
                     KPCLEIAREPRDVYKYTNKGNLVAVISNGTAVLGLGNIGAAAGKPVMEGKAVLFKRFA
                     DIDVFDLELDTRDTEAFINAVRIMEPTFGGINLEDIAAPECFEIEERLKAVMKIPVFH
                     DDQHGTAIISAAALKNALEVAGKKIEDVRIVISGAGAAAMACLRLYIKMGLSKENVML
                     VDSKGVIFKGRTIDMTPYKEEFAAETSRRTLTEALVDADVFVGLSVGGIVKAHMVQSM
                     AKDPIVFAMANPDPEIPYDDAVAARPDVIMATGRSDYPNQVNNVLGFPFIFRGALDCR
                     ATTINDEMKLAASLALAELAKEEVPDAVLRAYGNEKFTFGRLYLIPKPFDSRVLLNVP
                     VAVARAAAETGVAQEPITDYDAYRRRLEQLLGRARGMMHGVYDRAKSDPKRIVFPEGD
                     QEKIIRAAKILIEEGLAHPILLVRREVVAPLLQKHHVDESKVTIIDVETCAQRDQYVR
                     RFAELRMRQGITVADAQRVLNGRNYFAMMMVEAGDADGVIAGLRAYYPATIKPALQII
                     KTRPGVKNVSGAYLLMFKGKMIILADTTVIIEPTAEQLAEIAILTAETARRFNLEPRI
                     AMISFSNFGSTKHPNAEKVAEAVAIVKRLRPELIIEGEMQADAAVEPHIASADYPWSK
                     IQGDANVLIFPNLDSANAAYKLLWRLGGAEVIGPILQGMAKPVHVLQRGVDVSDIVNM
                     AAIAVVDAQDARS"
     gene            6289..7329
                     /locus_tag="VNN25_06410"
     CDS             6289..7329
                     /locus_tag="VNN25_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXH91195.1"
                     /translation="MPEIETVISSPVEARVLRVQHRAGDRLHAGDPILTLDTSATKLD
                     LDKLNDAVAQKESALMQARLKSEEDNAGARAALEQKQLDADILKLKAEENTRMHAEGL
                     VSSQDELVAVTAKKKNDIEITQLRDALGRVRRSTAAQIAAAQLELRTAQRERDQSARQ
                     LNLAMARSDQDGVLTWVLPEVGATVRKGDVIARVADLSAYRVVGTIADVHAAKLTAGM
                     RARVKLDDTTSIDGTISSIDPRIESGIAKFYVTLDQRAHPGLRNNLRVDVFPIMGSRN
                     NVLRVRRGALGGSEREDVFVVRDGMLVRTSVHWGLAGDEMVQPVEGLREGDNVVISNM
                     KDYEGIRELRMK"
     gene            7405..8070
                     /locus_tag="VNN25_06415"
     CDS             7405..8070
                     /locus_tag="VNN25_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91196.1"
                     /translation="MITLNNIDKVYRTERVETLALQNINLDIADGEFVSIMGPSGCGK
                     STLLNLIGLLDKPTNGKLMIDGQHINGAKDGSLAKMRNEKLGFVFQSFHLVPDLTVLD
                     NVEIPLLYRKMAASERKSRALAALDRVGLSSRVHHFPTQLSGGQQQRVAIARAIVGRP
                     TIVLADEPTGNLDSAMGDEIMGILEELNEKDGTTVVMVTHDDRFAERTVRTIRLFDGR
                     QVA"
     gene            8186..9424
                     /locus_tag="VNN25_06420"
     CDS             8186..9424
                     /locus_tag="VNN25_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91197.1"
                     /translation="MLKSSLKLAIKVLARRKVFTAISLVGIAMTLAVLMIATAVLDNI
                     LAPRAPQSNLDRIVSVSRVRKHGPHSTEQSNPGHGFLDPTIPNLPGTERVTYYKEIQT
                     VVVYDGQRRLELPYRRVDAEYWKVLDFHFLEGGPYSAADEAANRQVVVIAEDVRQKLF
                     GGMAAVGKMVNIGGEPHRVIGVVPTVPFTQQIAYSTLWVPLGPPTDQERKEFMGNFEV
                     SVLAKSTADIPRMQREFETRVSHLPIDDPKTYTEITSGLDTPFESIARALTNNELGSR
                     GPMVLRLVFLALALLFMTLPALNLITLNLSRILERASEVGVRKAFGAPRRMLIGQFIL
                     ENIVLTVIGGGAAFVLAFFMLQAFASIEFIPGAHFDLNLRVFGWGMLIAAFFGVFSGL
                     YPAWKMSRLDPVTALRGGAM"
     gene            9421..10614
                     /locus_tag="VNN25_06425"
     CDS             9421..10614
                     /locus_tag="VNN25_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91198.1"
                     /translation="MIRHLLKLVWRRKRANGLLIVEILFSFFVVFAVITMATSMIIRW
                     NKPLGFDYQNVWRASISFPPETEEQTDDSRRTTMAAMVRELQSQPQVEAVAGSGGPPY
                     STSTWTTDMTVNGHTIDVNVDTASDGFAKVLHVPILRGRWFTTDDDAAGNEHVIIDAD
                     VANSLFGTLDVVGKMLQPDKDTSYKIVGVIAPFRKHGELSQDHENMLFHRLSLTQKQG
                     RVGRDLIFRVRPGTPADFEETLIKRLHGIAPDYPVRIQHMDQMRVLLNKVFIAPAVIG
                     GIIASFLITMVALGLSGVLWQTVTRRTREIGLRRALGATGGEVNGQILIEVALLSTLA
                     VVVGVIVVAQLPLLGVFRFVSVPSFSIGLAGALATIYGLTLLCGVYPSWLAGRVQPAQ
                     ALHYE"
     gene            10771..12138
                     /locus_tag="VNN25_06430"
     CDS             10771..12138
                     /locus_tag="VNN25_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH91199.1"
                     /translation="MILIVDDDPSITASLALLLKQHGYRSASANSGDEALTRIRSERP
                     SLVIQDMNFSRRTSGEEGLELLTTIRTENPSLPVILMTAWGSIGLAVEGMKRGAADFI
                     TKPWSNALVVQSVETALKLAEAPEAASEAPSRADLDARFDIANIIGSDPKMLRVLELI
                     ARVAPTEASVLIMGESGTGKELVAEAIHGNSRRRGRPFVKVNLGGISSTLFESEMFGH
                     VRGAFTDARQDRKGRFEVADGGTIFLDEIGEIDPASQVKLLRVLQDRTYEVLGSSRTR
                     SVDLRVVSATNRPLHELVASGEFREDLLYRLNLITISLPALRDRRADVPRLASHFLRL
                     ASEIYERSVDGIAPRAMEWLKTQNWPGNIRQLRQTIERAVVMSNGGELTLDDFVAVRD
                     LESRQRRDASLPDAGTMTLDEIERGMIVKCLKHYEGNLSRVADALGLSRAALYRRLEK
                     YGIAS"
     gene            12230..13522
                     /locus_tag="VNN25_06435"
     CDS             12230..13522
                     /locus_tag="VNN25_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH91200.1"
                     /translation="MTLRTKLLLWFIVLHVALAAGAVLILMEHQDWLFAVELGFVVSI
                     VVSYRMVNALFVPLELIRTGAELMNERDFTSRFVPIGQPEMDRLIGIYNLMIDRLREE
                     RLAAEEQQHLLQKIVEASPSGIVICSFEGAVQQTNPAAERLLREPAVAATLPSLEAGQ
                     SRLVTLAGARRLKISRTEFRDRGFPKTFYLLEELTEELRITEKTAYEKLIRMMSHEVN
                     NSVGAVRSLLESVLHYAEQVGPEDRDDFTSALTIAAARIESLNRFMTGFADVVRLPPP
                     HTRDVDLGPIVDNIGRLLRPELENHAIALKTDVPAGVRVRVDQSQFEQVIINVIRNAV
                     ESIGERGEIEVCWLDGVLSVADSGHGVDASAAEEMFTPFFTTKREGRGLGLTIVSEIL
                     TNHHLPFSLTNREEGGAEFRVRFDSQIRGAGTPKTHSG"
     gene            13536..14327
                     /locus_tag="VNN25_06440"
     CDS             13536..14327
                     /locus_tag="VNN25_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91201.1"
                     /translation="MDSLRTLYRYRQLIATLTARDLKARYRGSILGFFWSLANPLLLL
                     AVYTIVFTFFFPSALIHPYPLFLFAGILPWTFFSAAVLESTSSISSNAGLIKKVMFPA
                     EALPLVTVASHLVHFVLAMPVLLLATLGFALHGDITISITILLVPVLMILQTLFVAGI
                     AMTVSSASVLFRDLRDILTNLMQLGFFVTPILYLVDRVQSRPLRALLRLNPMTPFVVS
                     YQDVLFFGHLPGLADTLLMIAYASFSVAMGVTVFGRLRDTLAEAI"
     gene            14324..15670
                     /locus_tag="VNN25_06445"
     CDS             14324..15670
                     /locus_tag="VNN25_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91202.1"
                     /translation="MSDYAIALTNVSKSYRLWGRGSQFATLKSALLKRDLALTPETSV
                     EAVRDVSFAVDRGEAFGIIGRNGSGKSTLLKLISGILKPTSGTVRVNGRVAALIELGA
                     GFHPEITGRENIYINGIMLGLSRREIEARFDQIVAFSGIPEFIDQPVKTYSSGMYVRL
                     GFAVAVHVDPDVLLIDEVLSVGDEEFSAKCVAKIQEMKYRGVTLVFVTHQLDQVRNLC
                     DRALWIDHGEPETIGDPVRVVDAYLQAVSSTQTAPARVADEAKAPTSAAPREDAGEEE
                     RWGSGEVVLSRVILVDSRGDELVALGPGTPVSIEMDVTVRVPQDDFVFGVGIYHADGT
                     CVYGTNTDLEGLMPERLETNGRVRFVMPALDLVAGTYRIDAAVHTRNGRAFDYRRGVI
                     RFVAGSRVHDIGVYRPKHHWKFEGGIAFREIDLEQRNVPVEVAEAMREASKPRGKV"
     gene            complement(16161..16343)
                     /locus_tag="VNN25_06450"
     CDS             complement(16161..16343)
                     /locus_tag="VNN25_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91203.1"
                     /translation="MESAVQNLTSEELAEFRSWFADYDGEAWDRQIEADSKAGKFDQL
                     SAEALAEFDRGETAEL"
BASE COUNT         3049 a         5014 c         5052 g         3231 t
ORIGIN      
        1 attcgctctt tgctggcgac gttctccagc gaagagcgaa tccgcgacgg ctcgatggtt
       61 ttccgcgaag gggaatcggg cgacaaactc tacatcgtcc tcgacggccg cgtgcgcatc
      121 tcaaaattca tcccaggcgt cggcgaggaa gcgctggccg tgctcgaccg cggcgacttc
      181 ttcggcgaga tggcgctgat cgacgacaaa gtacgctccg cggacgccaa agcgcacgac
      241 ggcgacgcca ccgttctctc gatcgaccgc gccactttga acgagatcct gtcgatggat
      301 ccgcacgcct cgctgcagtt cctcaacctc ctctgccgga tgatcagccg ccgccttcgc
      361 gagatcaacg agaagatcgt gcagtggaag tacatgagcg gcgggttctg agcgcctccc
      421 accgcaagtg acaaactcca acgaacgccc tagaatccaa aaaccacctc atgcgcgttc
      481 tgatcgtcgg tcagggggca cgcgaacatg ctctcgcgtg gaaactccgc cagtcaccct
      541 tagtcaggga ggtttacgcg gccccgggca atgccgggat cgctgagatc gccgactgcg
      601 tgaacatcgg cgtggccgac atcatcgaac tggctgattt cgccgagaag ctcaagatgg
      661 acctcaccat cgtcggtccc gagcttcctc tcaccctcgg catcgtcgac gagtttcaga
      721 aacgcggcat ggccatcttc ggcccgacgc gcctggccgc cgagctggaa ggctcgaagg
      781 cgttctccaa agagttcatg cgcaagtacg ggattccgac cgccgaggca agggtgtgct
      841 cgtcggcgga tgaggcgaag gcggcgctga aagagtttcg ctatccggct gtgatcaagg
      901 tggacggctt ggcgtccggc aaaggtgtcg tcatcgtcaa ctcgaaggcc gagggcgagg
      961 agtatctcaa gctcgtcttc gaggagaaga agttcggcaa cgccgccaac cgcatcctcg
     1021 tcgaggagtt cctcaccggc gaagaggtgt cgttcatggc gctgacggac gggaagaagt
     1081 tcgtctcgct cgcgcccgcc aaggactaca agaaagcctt cgacggcgag accggtccga
     1141 ataccggtgg catgggtgcg cactcgcccg cggtcgtcct gagcggggaa gcggccgccg
     1201 agatcgtgaa gacgatcatc atgccgacga ttcaaggcat ggccgaggag gggcgtccct
     1261 acacgggatg cctctacgcc ggcgtgatgc tgacgccgaa cggcccgaag gtgctcgagt
     1321 acaactgccg cttcggcgat cccgagacgc aggtgcagat gctccgcctc gaagacgacc
     1381 tcgccgacct ctgcctgaaa gtcgcccagg gcgacctcgg cgacaccaaa ctgacctgga
     1441 aaaaagaggc cgccgcctgc gtcgtcgtcg ccgtcgacgg ctaccccgac gagttcccca
     1501 aaggccagga agtatcgatc gatgccatcg acgacccgaa cgtcacgatc ttccacgccg
     1561 gcaccatccc ccgcaacggg aagatgatca acgtagccgg ccgcgtcgcc agcgtctgcg
     1621 cgaacgcacc gacgctctcc gaggcgctgg cgaaggccta cgaagccgcg ccgaaagtgc
     1681 ggttcgaagg ggcaaggttc cggaaggaca tcgggtaccg cgcgttggag cagaggaagg
     1741 ggtagacggc gtcattcacg tctgccgagc ccagtcgcca agtgagccaa gttctcacca
     1801 tctcgtccac gcggtagaat gacggacaga aaccgagatt acacaccgtg gccacatccc
     1861 actaccgcat tcagaaccac caacctgctc agacactcga cttgtacgag ggtcgggatc
     1921 cgcgggacgt tcccgcgtat tcgtttcgag aagtcgcgct cagcataggc gtgccgacgt
     1981 ctaccttgcg cgcctggttc gtcggtcaaa gcaactttga tcaggtgctg tcgcttccct
     2041 cggattggag cgagaatcag tcactatcgt tcttcaatct aatcgaagca aatgtggtcg
     2101 cggagcttcg gcgcgagcat ggagtggcaa tgaagcgcgt gcgcaccgcc ctggagttct
     2161 tgtctgacaa cattcctgtc gctcatccgc ttgtcgaaaa agagctgtta gtcacgccga
     2221 accagcgggt ctatatcgtt catgatggcc ggcgaatcga catttccgcc ggtggccaat
     2281 ggcctttgga tgtcgtagtc agaaatcttc ttcgacgggt tcaccgtggt cagcgtggca
     2341 ttctgacctt ctttcctcga gtcacgcctc ccagccacag cgttgtgctc gaagaggatt
     2401 acgcgccgat tgtcgtagat cctgaagtat gctttggccg gcctctgatt accggcaccg
     2461 caattcctgc ggatgtcgtc gcggaccggt acctgggtgg cgacagcatc ccgaagatgg
     2521 cggaggatta cgatctctcg gaagagcaaa tcgaagccgc gctccgattc gagcgattac
     2581 tcgaagctga cgcagcttga cctcccggga ttcacctatt tcttcgattc gacaaacatg
     2641 gtgcgcgagc ttgcggatgc tcttcatcag cgcggtcgcc tcgtgcacat gcacaacgaa
     2701 gtgtttccgg accggacacc cgacagagtc tggattctcg atgtagcaaa gcgaggctgg
     2761 atcattttga cgcgtgacaa gcgtctacag acaagacact tggagtggat cgcctttctt
     2821 cgcgcgaagg ctcgcgtctt gtggttcaaa ggtgaccgcg cgtcgaacgt tgagattacc
     2881 gagggttttc ttgctgcgct gcccaagatc gatcgcctcg ccctcagcct tccgcctcca
     2941 tacatcatca aaatcacgtc atcgggtcac gtcgagcaga tttaccccga aaagtagagc
     3001 tatattgttt acgcgacgac cctcacccga ccgtcttaac cgcgatcgtc ttcacattcc
     3061 tgatccatcg tgcgggccgt ttttcgccgg gaacgacgat tcggaacggt ccttccttct
     3121 cgggaagcgg tttgccgtct ttcttgtcgg caaggatgat gacgcggtcg ttgaaggcgg
     3181 gatcggtttc ggcgacggtg aagacgactt ggtacccgtc ggtggcggtg atgatgacga
     3241 tcgcgcgcag gcccgggccg cgaagtttct cgcccctagg gacgccggcg tgttcgagga
     3301 tcgcaccgag tgtgacgcct tcgtaggttg cggcggggtt ctcgtgctct ttggcttcgg
     3361 ctttcactcg cggcaagcct tccagcgtct tggcagtaac ggtgattgtt tgatcgggga
     3421 tgggctgtgc tgccgcgtgt tcggcggcga agagaggcgt cacggaagct aacgcgatca
     3481 gaaacatcgc tgttttcttc atcggttctc cttgaatctg atctcgtcaa acccgccagc
     3541 gggccgctgg cgatacagcc gacgggccgt cggcgctacg ttgccacagc ggtccgtcaa
     3601 tcaactgcgc gcgtcttgcg cgtctaccac cgcgatcgcg gccatgttca cgatatcgct
     3661 cacgtcgacg ccgcgctgta gcacgtggac cggtttggcc atgccttgca gaatcgggcc
     3721 gatcacttcg gcgccaccga ggcgccacag gagtttgtag gcggcattgg cggagtcgag
     3781 gttggggaaa atcaggacgt tcgcgtcgcc ctggattttg ctccaggggt agtcggcgct
     3841 ggcgatgtgc ggttcgacgg cggcgtcggc ctgcatttcg ccttcgatga tcagctcggg
     3901 gcggaggcgc ttcacgattg ccaccgcctc ggccaccttc tcggcattgg gatgcttcgt
     3961 cgacccgaaa ttagagaacg agatcatggc gatacgcggc tctaaattga agcgccgcgc
     4021 cgtctcggcg gtgaggatgg cgatctcggc gagctgctcg gcggtaggct cgatgatcac
     4081 cgtcgtgtcg gcgaggatga tcatcttccc cttgaacatc agcaagtacg cgccggagac
     4141 gttcttcacg ccaggccgcg ttttgatgat ctgcagcgcc ggcttgatcg tggcggggta
     4201 gtaggcgcgc aggccggcga tcacgccgtc ggcgtcgccc gcttccacca tcatcatcgc
     4261 gaagtagtta cgaccgttca gcacacgttg cgcgtcggcc acggtgatgc cctgccgcat
     4321 gcgcagttcc gcgaagcgac ggacgtactg atcgcgctgc gcgcaggttt cgacatcgat
     4381 gatggtcact ttcgattcgt cgacatgatg tttttgcagc aacggtgcga cgacctcgcg
     4441 gcggacgagc aggatgggat gggcgagtcc ttcctcgatc agaatcttcg cagcgcggat
     4501 gatcttttcc tgatcgccct cggggaagac gattcgtttg ggatcgctct tggcgcgatc
     4561 gtaaacgccg tgcatcatgc cgcgggcgcg cccgagaagt tgctcgagac ggcgtcggta
     4621 ggcgtcgtag tcggtgatcg gttcctgcgc gacgccggtc tccgcggccg cgcgcgccac
     4681 cgcgaccggt acgttgagca gcacgcgcga atcgaacggc ttcgggatca ggtagagccg
     4741 cccgaacgtg aacttctcgt tgccgtacgc gcgcagcacg gcgtcgggga cttcttcctt
     4801 cgccaattcg gccagcgcca gtgacgcagc gagcttcatc tcgtcgttga tcgtcgtggc
     4861 gcggcagtcg agagcgccac ggaagatgaa gggaaagccg agaacgttgt tgacctggtt
     4921 cgggtaatcg gagcggccgg tggccatgat cacgtcgggc cgcgcggcca cggcgtcgtc
     4981 gtatggaatc tccggatcgg gattcgccat cgcgaagacg atcgggtcct tcgccatcga
     5041 ttggaccatg tgcgccttga cgatgccgcc gaccgacagg ccgacgaaca cgtccgcatc
     5101 cacaagggcc tcggtcaggg tgcgccgcga tgtctcggcc gcgaactcct ctttgtacgg
     5161 cgtcatgtcg atcgtgcggc ccttgaagat cacgccctta ctgtccacca gcatcacgtt
     5221 ctctttgctc aatcccatct tgatgtagag ccgcaggcag gccatggccg ccgcgccggc
     5281 cccggagatg acgatgcgaa cgtcttcgat cttcttgccg gccacttcca gcgcgttctt
     5341 cagcgcggct gccgagatga tggccgtgcc gtgctggtcg tcgtggaaga ccgggatctt
     5401 catcacagcc ttgagccgct cttcgatctc gaagcactcg ggcgcggcaa tgtcctcgag
     5461 gttgatgccg ccgaacgtcg gctccatgat cctcaccgcg ttgatgaacg cctcggtgtc
     5521 gcgcgtatcg agctcgagat cgaagacgtc aatgtcggca aaacgcttga agaggacggc
     5581 cttcccttcc atcaccggct tgccagccgc cgcgccgatg ttgccgagcc cgaggaccgc
     5641 ggtgccgttg gagatgaccg cgacgaggtt tcccttgttc gtgtacttgt agacgtcgcg
     5701 cggctcgcgg gcaatctcga ggcagggctt ggccacgccg ggggtgtagg ccagcgcgag
     5761 gtcgcgcgcc gtcgcagtcg gcttggtcgg aacgacttcc gttttcccag gacgcccccc
     5821 cgagtggtac tccagagcgt catcgcggtc gttgtctgcc atcggttcga tctctcccgt
     5881 gaaacgcggg agtctatcaa catgcacggt tctgccctac caggcttgct caccgtgtgc
     5941 tgtgatccgc tcgggccacg caacatgtcc gattccgaac aatctctttc gaaagcgcac
     6001 ggagacgagg tgttgagagc gtcataactc ctttgttttc aataatccgt ccatggcatc
     6061 ccgcttggta tgtagcggtc tcacatggat cgcgaaatcg cacaggaagt ccggacgcgg
     6121 cgcacgacga agcgcgttgt gatcgcgata gtcgctatag cttcggtgat gttctgcgtc
     6181 gcggcaaccg ttagctggct gagaccttcg gtgaagcggt cagatattca gattgctcgc
     6241 gtggagcgcg ggtcggtcga tgcaaccctg caagctacgg ggacagtcgt gccggagatt
     6301 gagacggtca tctccagtcc ggtggaggcg cgggtgttgc gggttcagca tcgggcggga
     6361 gaccggcttc acgctggtga cccaatcctc acgctcgaca cctcggccac caagctcgat
     6421 ctcgacaagt tgaatgacgc cgtggcgcag aaggagagtg cgctgatgca ggcgcggctc
     6481 aagagcgagg aagacaacgc gggagcgcgt gcggcgctgg agcagaagca gctcgacgcc
     6541 gacatcctca agctcaaggc cgaagagaac acgcgcatgc atgccgaggg gctggtgtcg
     6601 tcgcaggacg aactggtggc ggtgaccgcg aagaagaaga acgacatcga aatcactcag
     6661 ttgcgcgatg cgctcgggcg cgtgcggcgc tcaacggcag cgcagatcgc ggcggcgcag
     6721 ctcgagctga gaacagccca gagggagcgc gatcaatcgg cgcggcaact caacctggcc
     6781 atggcgcgca gcgatcagga cggcgtactg acgtgggtgt tacccgaggt gggggcgaca
     6841 gtgaggaagg gagacgtgat cgcgcgcgtg gccgacctct cggcgtatcg cgttgtcggc
     6901 acgatcgccg acgtgcatgc ggcgaaactc acggccggca tgcgtgcgcg cgtgaagctg
     6961 gacgacacga cgtcaatcga cggcacgatc tcgagcatcg atccacgcat cgagagcggc
     7021 atcgcgaagt tctacgtcac gctcgaccag cgcgcgcatc caggccttcg caacaacctc
     7081 cgcgtcgatg ttttcccaat catgggcagc cgcaacaacg tgttgcgcgt gcggcgtggg
     7141 gcgctcggcg gcagcgagcg tgaggacgtc ttcgtcgtcc gcgacgggat gctcgtgcgc
     7201 acgtccgtgc actggggact tgccggcgac gagatggttc agccggtcga gggcctgcgc
     7261 gaaggcgaca acgtcgtgat ctcgaacatg aaggattacg aggggattcg tgaattgagg
     7321 atgaagtgag gtagccagcg gctagtggtt cggccaacca ctaactactg cattaatcag
     7381 aacgcaccaa accaggagac acacatgatc acactcaaca acatcgacaa ggtttaccgc
     7441 accgagcgcg tcgagacgct ggcgttgcag aacatcaatc tcgacattgc ggacggcgaa
     7501 ttcgtttcga tcatggggcc gtcgggctgc gggaagtcga cgttgctcaa tctgatcgga
     7561 ctgctcgaca agcccaccaa cggcaaactg atgatcgacg gacagcacat caacggcgcg
     7621 aaggatgggt cgctggcgaa gatgcgcaat gagaagctcg gcttcgtttt ccaaagcttt
     7681 catctcgtgc cggatctgac ggttctcgat aacgtcgaaa tcccgctcct gtaccggaag
     7741 atggccgcct ccgagcgcaa gtcgcgcgca cttgcggcgc tcgaccgcgt cggcctttcg
     7801 tcgcgcgtcc atcactttcc gacgcagctc tccggcggac agcagcagcg tgtggccatc
     7861 gcccgggcca tcgtcggacg gccaaccatc gtgctcgcag atgagccgac cggcaacctc
     7921 gactccgcca tgggcgacga gatcatgggc atcctcgagg agctgaacga gaaggacggc
     7981 acgaccgtgg tgatggtcac gcacgacgac cgattcgcgg aacggacggt gcggaccatc
     8041 cgcttgttcg atggacggca ggtggcgtga ggttcgggga gtggggtgtg gggcgtaggg
     8101 ggtcggagcc caacctacgg cctgctggct cccaatccca cccaattccc tctccccact
     8161 cccagctaca agaaggaaca acacaatgct taaaagctca ctgaaactcg cgatcaaggt
     8221 gctggcgcgg cgcaaggttt ttacggccat ttcgctcgtc ggcatcgcca tgacgctggc
     8281 agtgctgatg attgctacgg ctgtcctcga caacatcctt gcgccgcgtg cgccgcaatc
     8341 gaacctcgat cgtatcgtct ccgtgagtcg cgttcgtaaa catggaccgc actcgacgga
     8401 gcaaagcaat ccaggccacg gctttctcga tccgaccata ccgaatctgc cgggcacgga
     8461 gcgcgtgacg tactacaaag agatccagac agtcgtcgtg tacgacgggc agcggcgtct
     8521 cgagctgccg taccgccgcg tggatgccga gtattggaag gtgctcgatt tccatttcct
     8581 cgaaggtggt ccatacagcg cggcggatga agcggcaaac cggcaggtcg tcgtcatcgc
     8641 cgaggacgtg cgccagaagc tgttcggagg gatggcggct gtcgggaaaa tggtcaacat
     8701 cggcggcgag ccccatcgcg tcatcggtgt cgttcctacc gtgccattca cccagcagat
     8761 tgcgtacagc acgctgtggg tgccgctcgg tccgccgact gatcaggaga ggaaagagtt
     8821 catgggcaat ttcgaggtct cggttctggc taaaagcaca gccgatatcc cgaggatgca
     8881 gcgggagttc gagacgcgag tgtcgcatct gccgatcgat gatcccaaga cctacaccga
     8941 gattacttcg gggctggaca cgccgtttga atcgatcgcg cgtgccctga cgaacaatga
     9001 gctcggctca cgcggaccga tggttctgcg gctggtgttc ctggcgctgg cgcttctatt
     9061 catgacgttg cctgcgctca atctcatcac gctgaatctc agccgcattc tcgagcgcgc
     9121 ctcggaagtc ggagtccgca aggcgttcgg cgctccgcgg cgcatgctca tcggtcagtt
     9181 catcctcgaa aatatcgtcc tgacggtgat cggcggtggc gcggcctttg tgctcgcatt
     9241 tttcatgctt caggcattcg cttccatcga gttcatcccc ggcgcgcact tcgatctcaa
     9301 cctgcgcgtc ttcggctggg gaatgctgat cgctgcgttc ttcggcgtct tctccgggct
     9361 ttatcccgcc tggaagatgt cccgcctcga tcccgtcact gctctgcgcg gaggtgcaat
     9421 gtgattcgtc atctattaaa actggtgtgg cggcggaaac gcgccaatgg gctgctgatc
     9481 gtcgaaattc tcttttcctt ctttgtggtc ttcgcggtca tcacgatggc cacgtcgatg
     9541 atcattcgct ggaacaaacc gctcggcttc gactaccaaa acgtctggcg ggccagcatc
     9601 agttttccgc ccgagaccga ggagcagacc gacgactcac ggcgaacaac gatggcggcg
     9661 atggtgcgtg agcttcagtc ccagccccag gtcgaagccg tcgctggctc gggcggaccg
     9721 ccatacagca cctcgacgtg gaccaccgat atgaccgtca acggccacac gatcgacgtc
     9781 aatgtcgaca ccgcgagcga tggctttgcg aaggtgctac acgtaccgat actccgcggc
     9841 cgctggttca cgacggacga cgatgcggct gggaacgaac acgtgatcat tgatgcggac
     9901 gtcgcgaatt cgcttttcgg gacgctggat gtcgtcggca agatgctgca gcccgacaag
     9961 gacacgtcgt acaagatcgt gggagtgatc gcaccgttcc gcaagcatgg ggagttgtct
    10021 caggaccacg agaatatgct ctttcaccgt ctctctctga cgcagaaaca ggggcgtgtc
    10081 ggtcgcgacc tgatcttccg cgttcgcccc ggaacgccgg cggacttcga agagaccctc
    10141 attaaacggc tgcacgggat tgcgcctgac tatcccgtac gcattcagca catggaccag
    10201 atgcgcgtgc tcctgaataa ggtgttcatt gcgcccgcgg tcatcggtgg gatcatcgcg
    10261 tcgttcctca tcacaatggt tgcgcttggg ctctccggcg tactttggca aacagtcacc
    10321 cgccgcacgc gcgagatcgg cctccgacgc gccctcgggg ccaccggcgg cgaggtcaac
    10381 ggacagatcc tgatcgaggt ggctctgctc tcgacgctcg cggtggtcgt tggcgtgatt
    10441 gtcgtcgccc aactcccgct tctcggcgtc tttcgctttg tcagcgtgcc ctcgttcagc
    10501 atcgggctgg ccggcgcgct tgcgacgatt tatggcctca cgctactgtg cggcgtatat
    10561 ccaagctggc tggcgggccg cgtccaaccc gcgcaggcgt tgcattacga gtgagggtgc
    10621 cggttttcgg gggtcggggg tcggagatag agatcagagg atcagagact agcgatcagg
    10681 aaacgaaaac gatagctcgc ttgatatctg atctcttgtc tttgattctt ctcccacccc
    10741 ccactcccta ctccccacac cccaacaatc atgattctca tcgttgacga cgatccttcg
    10801 atcacggctt cactcgctct gcttctgaag cagcacgggt atcgatcggc gtctgcgaat
    10861 agtggcgatg aggcgctgac acgtattcgt tcggagcggc cttcgctcgt cattcaggac
    10921 atgaactttt cccggcgcac gagcggcgag gaggggctgg agcttctcac caccattcgc
    10981 acggagaatc catctctgcc ggtgattctg atgacggcgt ggggctcgat cgggctggcg
    11041 gtcgaaggga tgaagcgcgg tgctgccgac ttcattacca aaccgtggtc gaacgcgctg
    11101 gtcgtgcagt cggtggagac cgcgctgaag ctggccgagg cgcctgaggc tgcgagcgag
    11161 gcaccgagtc gggcggatct tgatgcgcga ttcgacattg ccaacatcat cggcagcgat
    11221 ccgaaaatgt tgcgcgtgct cgagctgatt gcgcgggtcg cgccgaccga ggcgtcggta
    11281 ctgatcatgg gggaaagcgg aacggggaag gagctggtgg ccgaggcgat ccacggcaac
    11341 agccgccgcc gtggccgccc cttcgtcaaa gtgaacctcg gcggcatctc atccactctg
    11401 ttcgaaagtg agatgttcgg ccatgtccga ggcgcgttca ctgacgcgcg acaggaccgg
    11461 aaaggacggt tcgaagtcgc ggacggcggc acgatcttcc tcgacgagat cggcgaaatc
    11521 gatccggcct cgcaggtgaa attgttgcgc gtcctgcagg accggacgta cgaggtgctg
    11581 ggatcgagcc gcacgcgatc ggtcgatctg cgcgtcgtct ccgccacgaa ccggccgctg
    11641 catgagctag ttgccagcgg cgagttccgc gaggatctgc tttatcgcct caacctcatc
    11701 acgatctcgc ttccggcctt gcgcgatcgg cgcgccgatg ttccgcgcct ggcgtcgcac
    11761 tttctgcggc ttgcgtcgga gatctatgag cgcagtgtcg acggcattgc gccgcgggcg
    11821 atggagtggc tgaagacgca gaactggccg ggtaacatcc ggcagctacg gcagacgatc
    11881 gagcgtgccg tcgtcatgag caacggcggc gagctgacgc tggatgactt cgtggccgtg
    11941 cgcgatctcg agtctcgtca gcgccgcgac gcgtcgctcc cggacgccgg aacgatgacg
    12001 ctcgacgaga tcgaacgggg catgatcgtg aaatgcctga agcactacga agggaatctg
    12061 tcgcgcgtgg ccgatgctct gggactcagc cgagcggcgt tgtatcgaag gctggagaaa
    12121 tacgggattg cttcgtgatc acaaagccat taaccactga ggcacagagg acacagagaa
    12181 aacctctgtg atctctgtgt ctctgtggtt cattgattct ccaaccacaa tgaccttgag
    12241 aaccaaactg ctcctctggt tcatcgtcct ccatgtcgca ttggcggcgg gggcggtgct
    12301 catcctgatg gagcatcagg attggctctt cgcggtcgag ctcgggttcg tcgtttcgat
    12361 cgtcgtcagc tatcgcatgg tcaacgcgct gttcgtgcct ctggagttga ttcgcaccgg
    12421 cgcggagctg atgaacgagc gcgatttcac ctcgcgcttc gtgccgatcg gacagccgga
    12481 gatggacagg ctcatcggga tctacaacct gatgatcgac cggctgcgcg aagagcggct
    12541 ggcggctgaa gagcaacaac atctcctgca gaagatcgtc gaagcgtctc cctcgggtat
    12601 cgtgatctgc agtttcgaag gtgcggtcca gcagaccaac ccggccgccg agcggctgct
    12661 gcgcgagcct gccgtcgcgg caacgctgcc gtcgctcgaa gcgggccagt cgcggctcgt
    12721 caccctcgct ggcgcgcgac ggctgaaaat ctcgcgcacg gagttccgcg atcgcggctt
    12781 ccccaagacg ttctatctgc tcgaagagtt gaccgaggag ttgcggatca cggagaaaac
    12841 cgcttacgag aagctgatcc gcatgatgtc gcacgaggtc aacaattccg tcggcgcggt
    12901 acgctcgctg ctcgaatcgg tgcttcatta cgccgagcag gttggccccg aggatcgcga
    12961 cgacttcacc agcgctctga ccatcgccgc ggcgcgcatc gagtcgttga atcgcttcat
    13021 gaccggcttc gccgacgtcg tccgccttcc tccgccccat acgcgagacg tcgacctcgg
    13081 cccgatcgtc gataacattg gcaggctctt acgtccggag ctcgagaatc acgcaatcgc
    13141 gttgaagact gacgtcccgg ccggtgtgcg ggtgcgcgtc gaccaaagcc agttcgagca
    13201 ggtcatcatc aacgtgatcc gcaacgccgt cgaatcgatc ggcgagcgtg gcgagatcga
    13261 ggtgtgctgg ctcgatggcg ttctttcggt cgccgacagc ggccacggag tcgatgcctc
    13321 ggcggccgaa gagatgttca cccctttctt caccacaaag cgcgaaggcc gcggcctggg
    13381 cctcaccatc gtctccgaga tcctgacgaa tcatcacctc cccttctcgc tgacgaaccg
    13441 cgaggaagga ggcgcggagt tccgcgtgag gttcgattcg caaattcggg gagccggcac
    13501 gcccaaaaca cactcgggat aaactcgact cactcttgga ctccctccga actctttatc
    13561 gctaccggca gctcatcgcc acgctcacgg cgcgtgatct gaaagcgcgc taccgcggct
    13621 cgatcctcgg atttttctgg tcgctggcga atccgcttct gctgctcgcg gtctatacga
    13681 tcgtcttcac cttctttttc ccatcggctc tgattcatcc gtatccgctg tttctattcg
    13741 ctggaatcct gccgtggacg tttttctccg ctgcggttct cgagtcgacg tcgtcgatct
    13801 cgtcgaacgc ggggctgatc aagaaagtga tgttccccgc cgaggcgttg ccactggtga
    13861 cggtggcatc gcatctcgtg cacttcgtgc tggcgatgcc ggtcctcctt ctcgccaccc
    13921 tcggctttgc acttcacggc gacatcacca tcagcatcac gatcctgctc gtgcccgttc
    13981 tgatgatcct gcagacgctc ttcgtggccg gtatcgccat gaccgtctcg tccgcgtcgg
    14041 tgctcttccg cgatctgcgc gacatcctga cgaacctgat gcagctcggc ttcttcgtta
    14101 caccgatcct ttacctggtc gatcgcgtcc agtcacgccc attgcgcgca ctgctgcggc
    14161 tcaatccgat gaccccattc gtcgtctcgt accaggatgt gctgttcttc ggacatctgc
    14221 cagggctcgc cgacacactg ctgatgattg cgtacgcgtc gttctctgtg gcgatgggcg
    14281 tgacggtgtt cggaagacta cgcgatacgc tggcggaggc gatatgagcg actacgccat
    14341 cgcgctgacc aacgtctcca agtcctacag actctgggga cgcggatcgc aatttgcgac
    14401 gctgaagagc gcgctgctga agcgcgactt ggcgctcacg ccggaaacga gtgtggaagc
    14461 ggtgcgcgac gtctcgtttg cagtcgatcg cggcgaggct ttcggcatca tcggccgcaa
    14521 cggttctggc aagtcgacgc tgctcaagct catctccgga atcctgaagc cgaccagcgg
    14581 gaccgtgcga gtcaatggcc gcgtcgccgc cctgatcgag ctcggggcgg gcttccatcc
    14641 ggagatcacc ggccgcgaga acatctacat caacggcatc atgctcggtc tgtcacggcg
    14701 cgagatcgag gcacgtttcg atcagatcgt cgcgttctcc ggcatccccg agttcatcga
    14761 tcaaccggtc aaaacctact cctcgggaat gtacgtccgc ctcgggttcg ccgtcgccgt
    14821 tcatgtcgat cccgatgtgc ttctgatcga tgaggtcctc tccgtcggcg acgaagagtt
    14881 ctccgccaag tgcgtggcca agatccagga gatgaaatat cgcggcgtga cgctcgtctt
    14941 cgtcacccac cagctcgatc aagtgcgcaa tctctgcgac cgcgcgctct ggatcgatca
    15001 cggcgagccg gaaacgatcg gcgatccggt tcgcgtcgtc gatgcctatt tgcaggccgt
    15061 gtcatcgacg cagaccgctc cggcgcgcgt cgccgacgag gcgaaagcgc ccacctcggc
    15121 cgcgccacgc gaggacgccg gcgaagaaga acgctggggg tccggagagg tggtgctctc
    15181 gcgcgtcatc ctcgtcgaca gccgcggcga tgagctcgtc gccctcgggc ccggcacgcc
    15241 cgtctcgatc gagatggacg tgacagttcg cgtgccgcag gacgatttcg tattcggcgt
    15301 cggcatctat cacgccgacg gcacgtgcgt ttacgggacg aatactgacc tcgaaggcct
    15361 gatgccggag cgcctcgaaa cgaacgggcg cgtccgcttc gtcatgcccg ccctcgacct
    15421 cgtcgccgga acctaccgca tcgacgccgc cgtccacacc cgtaacggcc gcgctttcga
    15481 ctaccgccgc ggcgtcatcc gcttcgtcgc cggctcccgc gtccacgaca tcggcgtcta
    15541 tcgtccgaag catcactgga agttcgaggg cggcatcgcg ttccgcgaga tcgacctgga
    15601 acagcggaac gtgccggtgg aagtggcgga ggcgatgcgg gaggcaagta agcccagggg
    15661 gaaggtctag cagtgcggac tcgagggttt acggtgcgga cccgagggtt ttccatgcgg
    15721 accctttggg tattctgtgc agacccggag ggtttaccgt gcgaacctga gggtttaccg
    15781 tgcaaccccg agggtttacc gtgcgaaccc gagggtttac cgtgcaaccc ccgagggttt
    15841 accgtgcaac cccgagggtt taccgtgcaa ccccaagggt ttaccgtgca accctgaggg
    15901 tttaccgtgc aaccccgagg ggttaccgtg caaccccgag ggtttaccgt gcgaacgcgg
    15961 ggttttaccg tgcgaacccg agggtttacc gtgcgagccg agctctcacc gcgcggactg
    16021 gacgatgctc cactagctca cgcggcccga tctgcaacca aagactcaac cacagaggcg
    16081 cagagatcac tgaggacagc ggcgactctg tgagctctgc gactctgtgg ttcagggcac
    16141 tttcgcgcaa gctgctggac tcaaagctca gcggtctcgc cgcgatcgaa ttctgccaaa
    16201 gcttccgcag agagctggtc gaacttgcct gctttcgaat ctgcctcgat ctgacgatcc
    16261 cacgcctcac cgtcatagtc ggcgaaccag gaccgaaact cggcgagctc ttcggaagtg
    16321 agattctgaa ctgcgctctc cagcgc
//
LOCUS       DATLVW010000198         3756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_135705, whole genome shotgun sequence.
ACCESSION   DATLVW010000198 DATLVW010000000
VERSION     DATLVW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135705"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VNN25_06455"
     CDS             <1..999
                     /locus_tag="VNN25_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH91204.1"
                     /translation="AFAVLFATFAITYRYAMWLLRPPTKRYWNRGWQLFFSRRYFAAN
                     VGNWFRRVTADFALNRFIFKRNQSRGVAHFFIMWGCILAFAITFPLVFGWIHFETLPG
                     DLDSYRTFVFGFATTSFKIHSAVAFLIFHGLVWSAFMVIAGVMLAMRRRMLDHGAAAL
                     QRFAEDFLPLVLLFAISITGLLLTASYTWMQGYGYDFLALLHAITVIFTLLYLPFGKF
                     FHIFQRPAQLGVSFYKDVAAKEGMAACRRCQQEFAPLLQINDLIEVERELGYRYETNA
                     PGVGHYQRICPSCRRSMLGIAQGELWRSGARGAGGGEWEINHSLSPTPHSPHPTPR"
     gene            1009..3322
                     /locus_tag="VNN25_06460"
                     /pseudo
     CDS             1009..3322
                     /locus_tag="VNN25_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
     gene            3325..>3756
                     /locus_tag="VNN25_06465"
     CDS             3325..>3756
                     /locus_tag="VNN25_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXH91205.1"
                     /translation="MTRIGNMEFFLDPNRCIGCQSCVAACSECDTHRGHSMIHLQYVE
                     RPISVQTVPVICMHCDSPTCAEVCPADAIKRTDDGVVQTARKPRCIACNNCVLACPFG
                     VPKMQTEFSLMMKCDMCYDRSSVGLKPMCASVCPSQALFFGT"
BASE COUNT          694 a         1171 c         1189 g          702 t
ORIGIN      
        1 gccttcgccg tactcttcgc tacgtttgcg atcacctacc gctatgcgat gtggttgctg
       61 cggccaccca cgaagcgcta ttggaatcgc ggctggcagc tcttcttcag ccggcgctac
      121 ttcgccgcca acgttggcaa ctggtttcgc cgcgtcacgg cggactttgc gctcaaccgc
      181 ttcatcttca agcggaatca gagccgtggc gtggcccact ttttcatcat gtggggctgc
      241 atcctggcgt tcgcgatcac gtttccgctc gtcttcggct ggattcactt cgagacgctg
      301 ccgggtgacc tcgactccta ccgcacgttc gttttcggct ttgccacgac gtccttcaag
      361 attcattcgg ccgttgcctt cctgatcttt catggcctgg tgtggtccgc attcatggtg
      421 atcgccggag tgatgctggc gatgcgccgg aggatgctgg accacggagc ggcagcgctg
      481 cagcgattcg ccgaagactt tctgccgctc gtgcttctct tcgcgatcag catcaccggg
      541 ttgctgctca cggcgagcta cacgtggatg cagggctacg gctacgactt tctcgcgctg
      601 ctgcacgcga tcaccgtcat cttcacgctg ctctatctgc cgttcggaaa gttcttccac
      661 atcttccagc ggccggcgca gctcggcgtg agcttctaca aagacgtcgc agcaaaggaa
      721 gggatggctg cgtgccggcg gtgccagcag gagttcgcgc cgctcctgca gatcaacgat
      781 ctcatcgaag tcgagcgcga gctcgggtac cgctacgaaa cgaacgctcc cggcgtcggg
      841 cactaccagc gcatctgccc atcgtgccgg cgctccatgc tcgggatcgc gcagggcgag
      901 ctgtggcgga gcggggcgcg gggagcaggg ggtggggagt gggagataaa tcattcgctg
      961 tctcccactc cccactcccc acaccccact ccccgataag gatcctaaat ggcaacgcta
     1021 ccgaaagaac aggatgtcat cgccaacgcg tggggaccgc atctcaaccg catggaggga
     1081 ccgcgcgtcg ataccggcgt ggagccagac aggattgtcc cgacgcattg ctgcttctgc
     1141 ggccagcaat gcggcatcaa tctcaaggtc aaagacaacc tcgttatcgg attcgagccg
     1201 cgctacgact ttccattcaa caaggggatg ctctgtccga aaggcgtcaa gcgctacctt
     1261 cagggcggcc atccggaccg gctcacgacc gcgctgcagc gcgatcctga tgccgcggga
     1321 gggttttctc cgatggcgta cgacgcggca atcgcgcgcg tggcgtcgga gattgagcgc
     1381 attcaatcga cctacgggcc gaacgcgttc ggcgtgcttt cgggcgcgag cctcaccacc
     1441 gagaaggcgt acctgatggg caagtttgcc cgtgtctgcc tgaagacgaa gtacatcgat
     1501 tacaacggac ggctttgcat ggtcagcgcc ggcgcggcca acaagaaggc gttcggcatc
     1561 gatcgcgcgg cggaatccct gggacgacat tttgaaggcc gaggtcgtgc tgattgccgg
     1621 atcgaacacg gcggagtgct cgccgatcac gacgaactac gtctggcagg cgcgcgagaa
     1681 tggcggcaag gtcatcgtcg tcgatccacg cattacgccg atcgcacgga cggccgacat
     1741 cttcctgccg gtgaagccgg gccgcgacgt ggcgctgttc aacggcatcc tccagctcat
     1801 gatcgagaac gactggatcg atcacgattt catcgagaag aacaccgtcg gcttcgaagc
     1861 agtcgcggag catgtcgcgc aatggacgcc gcgccgcacg gccgaggtga ctgggattgc
     1921 ggagaagtcg attcgcgccg cggcggaatt gtgggggacg gcgaagacga gcttcctcat
     1981 gcacgcgcgc ggaatcgagc accattcgca tggcgtcgag aactgcctct ccacgatcaa
     2041 catcgtgctc gcctccggcc gcatcggaaa gccgtactgc ggttacggaa ccgtcgtcgg
     2101 gcaggggaac ggacagggag gacgcgagca cggacagaag tgcgatcagc ttcccggttg
     2161 gcgcgacatc tcgaatcctg cgcatcgcga atacgtcgca ggcgtgtggg gcgtgaagcc
     2221 cgaggagatc cccgaggcgg gcgtcgactg ctacgagatg atgcggaaga tcgatgcggg
     2281 tgagatcaag ggactgcttc tgatctgctt caatccgaaa gtctcgctgc ccgacaacgc
     2341 tttcgtcacg cgcgcgctgg agaagctcga gttctttacg gtgatcgatt tcttcctgag
     2401 cgagaccgcg caatacgcgg acatcgtcct tcccggttcg ctgcacgaag aagacgaagg
     2461 cgtcgtcgcg caggtcgagg gccgcgtgat caagatcaac aaggccgtcg atccgccggg
     2521 cgatgccacg caggactggc gcatcattca ggacatcgcc aaagcgctgg gtcgctcgca
     2581 tggcttcacg ttcgagagcc cgcgcgcgat tttcgatgag ctccgcgtcg cgtcgaaggg
     2641 tggcgtggcc gattactacg gcatcacgta cgaaaagatc gaagcgcaga acggcgtttg
     2701 ctggccttgc ccgactgagg atcatcccgg tacgcagcgc ctgttcgagc cgggatcgtc
     2761 gaatccgatc gcgaacggca acgggccgtt ctattttcct gatggcaatg cccgcttcgt
     2821 agtgccggcg tatcgtccgc cggcagaaga ggtggacgag gcatatccgg tgattctcac
     2881 gaccggccgc gtggtgagcc agtttctctc cggaacacag acgcggcgca tcgggccgct
     2941 tgtcgatcaa tatccggagc cgcgcgtgga gatccatccg cggctcgccc agaagtacgg
     3001 aatcgcggat ggtgactgga cgacggtcga ctcgcgccgc ggcacgatca cgctgcgcgc
     3061 gcaggttgtg acctcgatcc ggcccgacac ggtgttcatt ccctaccact ggccgggccg
     3121 taagtcggcc aatcagctca ccatcgcggc gcaggatccc gtgtcgaaga tcccggagta
     3181 caaggtgtgc gccgtgcgtc tgcgcaagag cgagaagccg gccgatgatg ccgagacgac
     3241 ggcgccgggc gcggctggat cgaacattat gcgcggcggc acggacgacg tggcgcgcac
     3301 tgctgtgcag cagggaaggt gacgatgact cggattggaa acatggagtt cttcctcgat
     3361 ccgaaccgct gcatcggctg ccagtcgtgc gtggccgcgt gcagcgagtg cgatacgcac
     3421 cgcggccact ccatgatcca cctgcagtac gtcgagcgtc cgatctccgt gcagaccgtg
     3481 ccggtcatct gcatgcactg cgattcgccg acgtgcgcgg aggtctgtcc ggccgacgct
     3541 atcaagcgca cggacgacgg cgtcgtgcag acagcgagga aaccgcgctg catcgcctgc
     3601 aacaactgcg tcctggcctg tcccttcggc gtgccgaaga tgcagaccga gttctcgctg
     3661 atgatgaagt gcgacatgtg ctacgaccgc tcttccgtcg gcctcaagcc gatgtgcgcc
     3721 agcgtctgcc ccagccaggc cctcttcttc ggcacc
//
LOCUS       DATLVW010000199         9598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_586308, whole genome shotgun sequence.
ACCESSION   DATLVW010000199 DATLVW010000000
VERSION     DATLVW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586308"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VNN25_06470"
     CDS             complement(<1..1403)
                     /locus_tag="VNN25_06470"
                     /inference="COORDINATES: protein motif:HMM:NF023805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HXH91206.1"
                     /translation="MRIIARLLTAFVLVTATSSVLAVTYIVPTDRDLVRRSEAIVVAT
                     AVSSHSQCTVDRRIVTVITLAVEESFKGEIDSRSSIDLVEPGGFAGDRGTIIPGSPRY
                     ADGKRYMVFLRRTPDGWATYGFGLGKFEWIDDAYGDQFLTRGGTVDDIFGWDELTSLP
                     HVEKLRDRAAFQSFVRSLVANAGAPVREDYFVAREEPASAGFQSLVPNPEVSVPGGLD
                     GVGAMTAGTSNWTGAGFGVHYNVGASNPSATGGLSHADGINAVLFNDPSGIVPNGVAA
                     LGGQSAVTGGNIITEVDVVVGKNFSTNQATFNGLMTHEMGHTLGFRHSDKTADDSGPC
                     VSPSPCSSNAIMNSTIAFNLQTLQQWDLDAVRAVYGGGGNASDYLFPGNPHWSSPSAS
                     FDYCFGPTITGQPSSTSIVSGAQTTLNVTGTGTNAAFQWYIGNPPSTTTTASNGTTSS
                     LTVSPTVTTTYWVRVTA"
     gene            complement(1475..3874)
                     /locus_tag="VNN25_06475"
     CDS             complement(1475..3874)
                     /locus_tag="VNN25_06475"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH91207.1"
                     /translation="MAFLLYLIAASLLLWISHRALVPLSRWAVIALLLLPLCVTGKAL
                     LTGGVFGPVDLPYFTEPLRDMRVPLGVPQPHNGMLSDLYAQMIPWRKAVQFALAHGQW
                     PLWNPFILSGTQLAASAQPAAYSPFTLIACLLPVAQSITFTAALTFFLAGLGAFLFAR
                     ELECRESVALSAAAGWMYAGPMAFFVLWSLGNSWALFPLVLFATRRSVHLPGIRSTAI
                     LSTTLALLLLAGHPETAFHIVFCGSLYAIVEMLVLRRNIVRPLASALAAGVIALLLCA
                     IYILPIFEAAPQTMEQQFRTGGYLDVPRGVGGVESGARILTDLFPHLHGRYWNWKDPH
                     WVPLETSAVGSILLALAAYAIIRVRRREVAFFAALALFGILARAAWAPLAAALQKLPL
                     FDITLNERFCFAAAFSLVILGALGLEHALRRRDRALFYTLAAAMLFFAAGAFTINRLN
                     IMAHSLPEWGVYTLFGELTCLGVGAILIAILWSRDRTTAPATEQTSNRTAFATTTAIL
                     ILIVIQRWQAGNDIYPTLPAFAAYPPVPILRSLPRETGEPFRIVGRAHALVPGTSALY
                     ELEDARGYEALTFSRYVATYDAWCKHQPVWFNRVDDLTKPFLSMLNVRYAIASDKLPA
                     PDGWRSVAVQRGAELLENTRVLPRAFVPERAVFGTSFDGTLDAMKAATDFRQRAWIEA
                     PLAAGERGNAGCTVASVRRDDGDLIVEAATSAGGWVVITEPAWDGWRVYIDGRRVQHF
                     YANVAFLGVFVPPGGHTIRLTYLPESFVRGRAISFSTLALLLGGGVFARLRRRRSLKP
                     V"
     gene            4094..6544
                     /locus_tag="VNN25_06480"
     CDS             4094..6544
                     /locus_tag="VNN25_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH91208.1"
                     /translation="MKSLVTRVLFCVAVLAIANPGPQLFAQQTPEQKAADEKKAAQEK
                     AAKEQQPPKASEEIVVTARKREETVQDVPISVAAPTASELRDRGAETMEDIAANVSGF
                     TVQNLGPGQSQVAIRGVSAGQIVRDQPGVKEQVGVYLDESVISLSLFTPDIDLFDMNR
                     VEVLRGPQGTLFGSGSLSGTVRYISNQPKLGTSETVGDFSVSSISNGSIGSSIKASVN
                     APLSDTSAMRVAAYYTRYGGFMDAVQPDLSVKKDVNDGDRTGARVAFRFEPDKKLTIT
                     PRILYQEVKMNGWNRADAFNILANPFTTTRPKVTLGPRELFTQTKEPFTDKFTLGDFN
                     VGYDLGGGKNLTSITSYTNRDVLVVRDATALTASITGGSIGLPPSIYTLTAPLDDATK
                     ARAFTQELRLSGGDKKLQWLVGGFYGHTDRKYGQSLLVSGFENLSKIPTAGTFGAAKD
                     VLFFSNLKYNFKQTALFGEATYSVSDKFDLTGGLRWYDFNENRTQTFDGIFADPGTTK
                     GTSKANGFAPRVIAAYKLNDDTKLNAQISKGFRLGGINDPLNVPLCTPGDLLTFGGHD
                     SWKDEQLWNYEVGSKSRVAGGRGSVNASAYYADIKDLQATVTAGSCSSRVIFNVPKAK
                     AAGAELEWELAPTDDFDFALSASHTDSKLQSTLFSTDKAGVKSIVAGIKSGNRLPTVP
                     QNQAAAAATYHWRSTQSWAGYATGAYQYIGSRFTQIGDQDPSFGTVNLLSFAPNNIGG
                     PFTQNTFTFNPELPAYQIVNLRVGVLKNKWDVALFVNNVFDERALLALDQERGTRARV
                     GYLVNQPRTIGISSRVNF"
     gene            6545..7795
                     /locus_tag="VNN25_06485"
     CDS             6545..7795
                     /locus_tag="VNN25_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH91209.1"
                     /translation="MANISRRTFLASAAALPVAARLGARTSTMSPSPRIAVVGAGAFG
                     GWTALQLQRLGAKVELIDAWGPGNARSSSGGDTRVIRAIYGTDRTYVEMVKRAFALWD
                     EIHISSDEPLYVATGVLWMNRGDDAYVRSSLPILESLGFPVEKLEVSEAAHRYPQIDF
                     HGIKSVWLEKRAGALSAHRACVAVRDAFEKAGGTYRTASAKPGAIVNGSMTGLLVDGS
                     RVETDAYVFACGPWLGRVFPDVVGDRILPTRQEVFYFGVPSGSERYEPAHLPIWIDFG
                     PRIIYGLPDIHGRGFKVADDTRGVHFDPTNGDRKASAEGLAHARHFLGERFPELADAP
                     LLSAEVCQYENSPDGNLIIDQHPGAKNVWLLGGGSGHGFKLSPAVGEMTAEAILLGKD
                     VPKMFRLARLAAMTKRSTQFDRKK"
     gene            complement(7897..9096)
                     /locus_tag="VNN25_06490"
     CDS             complement(7897..9096)
                     /locus_tag="VNN25_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXH91210.1"
                     /translation="MVTWLSAKDASSFLGVSRTTLYAYVSRGLVRSSASPGNSHLRRY
                     AAEDLERLRGRRELRRDPGKTAERALHWGVPIMESAITLIESDRLYYRGHDACELAES
                     RTVEEVAALLWTGGFETELFAATPLHVVAGGESADALPFVSRAQSMLPLVAVHDSLAF
                     DLRPRAVAQTGWRIVNLLTSVAVESSELEESIEETLQKRWVPRTAHALEILRAALILC
                     ADHELNVSSFTARCVASAQSNPYAVVVAGLAAMEGARHGGMSEKVETMLDELRRSRDV
                     RKALSDRLRRGELIHGFGHRLYPSGDPRAALLQEMLGRRFAKSPELTFVRSVAKAGEE
                     LTGDKANIDFALVAVERTLKLPRGSALTLFALGRSIGWIGHAIEQYANDEIIRPRAKY
                     VGEAPKG"
     gene            9173..>9598
                     /locus_tag="VNN25_06495"
     CDS             9173..>9598
                     /locus_tag="VNN25_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXH91211.1"
                     /translation="MSNPIIHNGLEGIVASETELSMVDGLAGELIIVGFPVGELAMNA
                     TFEETAFLLWYGRLPDARELAAFRADLASRRELPQAALDLLRAAADKQVDTMDALRMA
                     AGTISLRGSSKNDDDAARDLLAAFPTIVASYWRLLRAHDP"
BASE COUNT         1907 a         3051 c         3044 g         1596 t
ORIGIN      
        1 cacgcggtca cgcgaaccca ataggtcgta gtcacggtcg gagaaacggt caacgacgac
       61 gtcgttccgt tggaagctgt tgtcgtggtg ctcggaggat tgccgatgta ccactgaaac
      121 gccgcattgg tgcccgtccc agtgacattc agcgtggtct gcgcgcccga tacgatcgac
      181 gtgctggatg gctgccctgt gatcgtcggg ccgaagcagt agtcgaagct cgcgctcgga
      241 ctcgaccagt gaggattgcc gggaaagagg taatcggagg catttccccc tcctccgtaa
      301 acagcccgca cagcatcgag atcccattgc tggagcgtct gcaaattgaa ggcaatcgtt
      361 gagttcatga tcgcgttcga gctgcaaggg ctcggggaga cgcagggtcc cgagtcgtcc
      421 gccgtcttgt ccgaatggcg gaacccgagc gtgtgtccca tctcatgcgt catgagtccg
      481 ttgaacgtgg cttgattcgt ggagaaattc ttgccgacga cgacgtcgac ttccgtgatg
      541 atgtttccgc cagtcaccgc gctttgaccg ccgagggcgg caacgccgtt cgggacgatg
      601 ccgctcggat cattaaaaag caccgcgttg atgccgtcgg catggctcaa gccaccggtg
      661 gcgcttgggt tcgaggcgcc gacgttgtaa tggacgccga aaccagcgcc ggtccagttg
      721 ctggtgccgg ccgtcatcgc gccaaccccg tcgagtcctc ctggaaccga gacttccgga
      781 ttgggaacaa gcgactgaaa ccctgcggat gcgggttctt cgcgagcaac gaagtaatcc
      841 tctcgaacgg gtgcgcctgc attcgccacg agcgagcgga cgaaggattg gaatgccgca
      901 cgatcacgca acttctcgac gtgaggcaga ctcgtgagct cgtcccagcc aaagatgtcg
      961 tcgacggtcc cgccgcgcgt caggaactga tcgccgtacg cgtcatcgat ccattcgaat
     1021 ttgccgaggc caaatccata cgtggcccag ccatcgggcg tgcgtcgcag gaagaccatg
     1081 tagcgcttgc catcggcata gcgcggagaa ccgggaatga tcgtgccgcg gtcaccggca
     1141 aagccacccg gttcgacgag atcgatcgat gagcgtgaat cgatctcacc cttgaatgat
     1201 tcttcgactg ccagcgtgat gacagtgacg atgcgcctgt ctaccgtgca ttgggagtgc
     1261 gaactgacgg ccgtggcgac gacaatcgcc tccgatcgcc tcaccaggtc ccgatccgtc
     1321 ggaacgatgt aagtgaccgc aagaacagac gaggtggctg taaccagcac gaatgccgtc
     1381 aacagccggg caatgattcg catgggggcg tcctccgagg accgggcgtg agcacgattc
     1441 taggaactta atccccggaa aagtgtgccc ggggtcacac cggcttcagg gaacgacggc
     1501 gccgtagccg cgcaaacaca ccccctccga gcagcaacgc cagcgtggag aacgagatgg
     1561 cgcggccgcg aacgaaactc tccggcaggt acgtcaggcg aattgtgtgg ccgcccggcg
     1621 gaacgaagac gccgaggaag gctacgttgg cgtagaaatg ctgcacgcgg cggccgtcga
     1681 tgtagacgcg ccagccgtcc caggccggtt cggtgatcac gacccagccg ccggccgacg
     1741 ttgccgcttc gacgatcagg tcgccgtcgt cgcgccgcac actggccacc gtgcatccgg
     1801 cgttgccgcg ctctccggcg gccagcggcg cttcgatcca ggcgcgctga cgaaaatcgg
     1861 tcgctgcctt catcgcgtcg agcgttccgt cgaacgacgt gccgaacacg gcgcgctcgg
     1921 ggacgaacgc gcgcggaaga acgcgcgtgt tctccagcag ctccgcgcca cgttgcacag
     1981 ccacgcttcg ccagccatcg ggcgcgggca gcttgtccga ggcgatggcg tagcggacgt
     2041 tcaacatcga gagaaacggt ttggtcagat cgtcgacgcg gttgaaccac accggctgat
     2101 gcttgcacca ggcgtcgtac gtcgctacgt agcgcgagaa ggtcagcgcc tcgtacccgc
     2161 gcgcgtcttc gagctcgtag agcgcgctcg ttccgggaac cagcgcgtgc gcgcggccga
     2221 cgatgcggaa cggctctccc gtctcccgcg gtaacgaccg gaggatcgga acaggcggat
     2281 acgccgcgaa cgcgggcaac gtcggataga tgtcgttgcc ggcctgccag cgttggatga
     2341 cgatgaggat gaggatggct gttgtcgttg cgaaggctgt ccggttgctg gtttgctcgg
     2401 ttgctggtgc ggtcgtgcgg tctcgcgacc acaaaatcgc gatcaaaatc gctcccacgc
     2461 cgaggcaagt cagctcgccg aacagggtgt agacgcccca ctcgggaagg ctgtgagcca
     2521 tgatgttgag ccggttgatc gtgaaagcgc cggcggcgaa aaagagcatc gctgcggcca
     2581 gggtgtagaa aagtgcgcgg tctcgtctgc gcaatgcgtg ctcgagtccg agtgcgccga
     2641 ggatgacgag ggagaaggcg gcggcgaagc agaatcgttc gttgagcgtg atgtcgaaga
     2701 gcgggagctt ctgcagcgct gccgcaagtg gcgcccacgc ggcgcgcgcg agaattccga
     2761 agagcgccag cgccgcgaag aaggcgacct cgcgccgccg cacgcggatg atcgcgtacg
     2821 cggccagcgc caggaggatg ctgcccaccg ccgaggtctc caacggcacc caatgcggat
     2881 ctttccagtt ccaataccgt ccgtgcagat gcgggaaaag atccgtgagt atccgcgccc
     2941 cgctctcgac cccaccgact cctcggggga cgtcgaggta gcctccggtg cggaattgct
     3001 gctccatcgt ctgcggcgcc gcttcgaaaa tcggaaggat gtagatcgca cacagcagca
     3061 gcgcaatcac gcccgcggcc agagcgctgg cgagcggacg gacgatgttg cgccgcaaca
     3121 ccagcatttc cacgatcgcg taaagcgatc cgcagaagac gatgtgaaac gcggtttccg
     3181 gatggccggc cagcagcaga agggcgagtg tcgtcgacag aattgcggtc gaccggatgc
     3241 cgggcagatg caccgagcgc ctcgtggcga agaggaccag aggaaagagc gcccaggagt
     3301 tgccgaggga ccagaggacg aagaaggcca tcggtcccgc atacatccac ccggcagcgg
     3361 cagagagcgc gactgactcg cggcactcga gctcgcgagc gaagagaaac gcgcccagtc
     3421 cggccagaaa aaacgtcagc gcggccgtaa aggtgatgct ttgcgcgacc ggcagcaggc
     3481 aagcgatcag cgtgaacggc gagtacgcgg cgggctgcgc cgaggccgcg agctgcgtcc
     3541 ccgagagaat gaagggattc cagagaggcc actggccatg cgcgagcgcg aactgcacgg
     3601 ccttccgcca cgggatcatc tgcgcgtaca gatcggacag catcccgttg tgcggctgcg
     3661 gcacgcctag cggcacgcgc atgtcccgca gcggctcggt gaaatacggg agatcgaccg
     3721 gcccgaacac gcctcccgtc agcaacgcct tgccggtgac gcagaggggc aggagcaaga
     3781 gtgcgatcac cgcccagcgc gagagcggaa cgagcgcacg gtgcgagatc cagagcagca
     3841 gcgatgctgc gatgaggtac agcaggaatg ccatgagcgg cggagtgtac cggaggtgag
     3901 gtctgcaagc tcgaatcgaa acagaggcgg ttcacgcgga gatgcggagg aaaagactcc
     3961 gcgccctccg agtctccgcg tgaaaaaggt tctggacgta aagcaacccc aaatcccttg
     4021 acctcgaact gcgcagagga tgacaatcgg cctcatcaat acatagcagc gtgaaaacgc
     4081 gggaggaagc atcatgaaaa gcctcgtgac gagagtactt ttttgcgtcg cagtcctggc
     4141 aatcgccaac cccggtccac aacttttcgc gcagcagacg ccggagcaga aagccgcgga
     4201 cgaaaagaag gccgcgcagg aaaaggctgc aaaggaacaa cagccaccca aggcgagcga
     4261 agagatcgtg gtcacggctc gtaagcgtga agagacggtg caggacgttc ccatctccgt
     4321 ggctgcgccg acggcatccg agctccgcga ccgcggcgcc gagacgatgg aagacatcgc
     4381 cgccaacgtc tccggcttca ccgtgcaaaa cctcgggcct ggacaaagcc aggtggccat
     4441 ccgcggcgtg tccgcgggtc agatcgttcg cgaccaaccc ggcgtcaaag agcaggtcgg
     4501 cgtctacctc gatgagtcgg tgatctcgct ctcgctcttc acgccggata tcgacttgtt
     4561 cgacatgaac cgcgtcgaag tccttcgcgg accccaaggc acactgttcg gttccggttc
     4621 gctctcggga acggtgcgct acatcagcaa tcaaccgaag ctcggcacct ccgagacggt
     4681 cggcgacttc agcgtcagct cgatcagcaa cggcagcatc ggatcgagca tcaaagcctc
     4741 ggtcaacgcg ccgctgagcg acacctcggc gatgcgggtc gccgcgtact acacgcgcta
     4801 cggcggcttc atggatgcgg tgcagcccga tctcagcgtg aagaaggacg tgaacgacgg
     4861 cgaccggacc ggcgcgcgcg tggcgttccg cttcgagccg gacaagaagc taacgatcac
     4921 tccgcgcatc ctctaccagg aagtgaagat gaatggctgg aaccgagccg acgccttcaa
     4981 catcctggca aaccccttca ccaccacgcg tccaaaagtc accctcgggc cgcgcgagct
     5041 tttcacgcag accaaagagc ccttcaccga caagttcacg ctcggcgact tcaacgtcgg
     5101 ctacgacctc ggcggcggca agaacctcac gtccatcact tcctacacca accgcgacgt
     5161 gctcgttgtc cgcgatgcca ccgcgttgac agccagcatc accggcggca gcatcggcct
     5221 gccgccaagc atctacacgc tcactgcgcc gctcgacgac gccacgaaag ccagggcgtt
     5281 cacacaggag ttgcgtctct cgggcggcga caagaaactg cagtggctgg tcggcggctt
     5341 ctatggccac accgaccgca agtacggcca gagcctgctc gtcagcggat tcgagaacct
     5401 ctcgaagatc ccgactgccg gtactttcgg cgcggcaaag gacgtcctct tcttctccaa
     5461 tttgaagtac aacttcaaac agactgcgct gtttggtgag gcgacctact cggtcagcga
     5521 caagttcgat ctgaccggcg gcctgcgctg gtacgacttc aacgagaatc gcacgcagac
     5581 attcgacggc atcttcgccg atcccggcac gacaaagggc acgtcgaagg ccaatggctt
     5641 tgccccgcgc gtcatcgccg cgtacaaact caacgatgac accaaactga atgcccagat
     5701 ctccaaaggc ttccgcctcg gcggcatcaa cgatccactc aacgtgcctc tctgcacgcc
     5761 gggtgatctc ctgacgttcg gcggccacga tagctggaag gacgagcagc tctggaacta
     5821 cgaggtgggc tcgaagtcgc gcgtcgccgg tggacgcggt tcggtcaacg cctcggcgta
     5881 ctacgccgac atcaaggatc tgcaggcgac cgtgaccgcc ggctcctgct cctcgcgcgt
     5941 catcttcaac gtcccgaagg caaaagccgc aggcgcggag ctggagtggg aactggcgcc
     6001 gacggacgat ttcgatttcg cgctctcggc aagccacacc gactccaagc tgcagtcgac
     6061 actgttctcg accgataagg cgggcgtgaa aagcatcgtc gccggaatca agtccggcaa
     6121 ccggctgccg acggtgcctc agaaccaggc tgccgcggcg gccacgtacc actggcgctc
     6181 gacgcagagc tgggcgggat atgcaacggg cgcttaccag tacatcggct cgcgcttcac
     6241 gcagatcggc gatcaggacc cgtcgttcgg cacggtgaac ctgctctcgt tcgcaccaaa
     6301 caacatcggt ggcccgttca cacaaaacac attcaccttc aatccggagt tgcctgcgta
     6361 tcaaatcgtc aaccttcggg tcggcgtact gaagaacaaa tgggatgtgg cgctcttcgt
     6421 caacaacgtg ttcgacgagc gagcgttgct ggcgctcgat caggagcggg gaacccgcgc
     6481 tcgtgtcggc tatctggtca atcagccgcg cacgatcggc atcagctcac gcgtgaattt
     6541 ctagttggcg aacatttccc gcaggacctt tctcgcgagc gctgcggcgc tgccggttgc
     6601 ggcacgcctc ggcgctcgca cttccaccat gtcgccatcg ccacgcattg ccgtcgtcgg
     6661 cgcgggtgcg ttcggaggat ggacagcgct ccagctccag cggctcggcg ccaaggtaga
     6721 gctgatcgac gcctggggtc cgggaaacgc gcggtccagc tccggcggcg atacccgcgt
     6781 catccgcgcg atctacggca ccgatcgcac gtacgttgag atggtgaagc gggccttcgc
     6841 gctgtgggat gagatccaca tctcatccga cgagcctctg tacgttgcga caggcgtcct
     6901 ttggatgaat cgcggcgatg acgcctacgt tcgctcgtcg ttgcccatcc tggaatcgct
     6961 cggctttccc gtcgagaagc tcgaggtctc cgaggctgct caccgatatc cgcagatcga
     7021 ttttcacggg atcaagtccg tctggctcga aaaaagggca ggtgccttat ccgcgcaccg
     7081 cgcctgcgtc gcggtccgcg acgcgttcga aaaggccggc gggacgtacc gcaccgccag
     7141 cgcaaaacca ggcgcgatcg tcaatggctc gatgaccggc ctcctcgtcg atggatcgcg
     7201 tgtcgagacg gatgcgtatg ttttcgcctg tggtccctgg ctcggccgcg tcttccccga
     7261 cgtcgtcggc gaccgtatcc tgccgacgcg gcaggaggtc ttttacttcg gcgttccctc
     7321 gggttcggag cgctacgagc cggcccatct tccgatctgg atcgatttcg gcccgcggat
     7381 catctacggt cttcccgaca tccacggccg cggattcaaa gttgccgacg acacgcgcgg
     7441 cgtgcacttc gatccgacca acggcgaccg caaggcgagc gccgaaggct tggctcacgc
     7501 acgccatttc ctcggagagc gcttcccgga gctggccgac gcgcctctgc tgtcggccga
     7561 agtctgtcag tacgaaaaca gccccgacgg caacctgatc atcgaccagc atcccggcgc
     7621 gaagaatgtc tggctgctcg gcggcggctc agggcacggc ttcaaactat cccccgcggt
     7681 gggagagatg accgccgagg cgatcctgtt ggggaaggac gtcccgaaga tgttccgtct
     7741 cgcccggctc gccgcgatga cgaagcgatc gacgcagttc gatcggaaga agtagagatg
     7801 ctcacacgag aaatcaccac agagacacag agagcacaga ggtttcgcct catctctgtg
     7861 tcctccgtgt ctctgtggta atggtctttg aaaacgtcaa cccttcggcg cctctcccac
     7921 gtatttcgcc cgcggccgga tgatctcgtc gttggcgtac tgctcgatgg cgtggccgat
     7981 ccatccgatg ctgcggccga gggcgaagag ggtcagggcg gagccgcggg gaagtttgag
     8041 cgtgcgctcc accgcaacga gggcaaaatc gatgttcgct ttgtctccgg tcagctcctc
     8101 cccggccttg gccacgctgc ggacaaacgt cagctcgggc gatttggcga agcggcggcc
     8161 cagcatttcc tgcagcagcg cggcgcgagg atcgccgctc ggatagagac gatgtccgaa
     8221 gccgtgaatc agctccccgc ggcgcaaacg gtccgacagc gctttacgga cgtcgcgtga
     8281 gcggcgcagc tcgtcgagca tggtctcgac tttctcgctc atgccgccgt gccgtgcccc
     8341 ctccatcgcc gccaggcctg ccacgacgac cgcgtaagga ttcgactgcg ccgaggcgac
     8401 gcagcgggcg gtgaaggaag agacgttcag ctcgtggtcc gcgcagagaa tcagcgccgc
     8461 acgcagaatc tcgagcgcgt gcgccgtgcg cggaacccat cgtttctgca gcgtctcttc
     8521 gatcgactct tccagctcgg acgactccac cgccacgctg gtgagcaggt tgacgatccg
     8581 ccagccggtc tgggcgacgg cgcgcgggcg taggtcgaag gcgagcgagt cgtgcacggc
     8641 gaccagcgga agcatcgact gcgcccggct gacgaacggc agcgcgtcgg cactctcccc
     8701 tccggcgacg acgtgcaacg gagtggcggc gaagagctcg gtctcgaagc cgccggtcca
     8761 gaggagagcg gcgacctcct cgacggttcg gctttcggcg agctcgcagg catcatggcc
     8821 gcggtagtaa aggcggtcgg actcgataag cgtgatcgcc gactccatga tcggcacgcc
     8881 ccaatgaagg gcccgttcgg cggtcttgcc gggatcgcgg cgaagctcgc ggcgtccgcg
     8941 caaccgctcg aggtcttccg ctgcgtagcg ccggagatgc gagtttccgg gggatgccga
     9001 cgagcgcacc aggccgcggc tcacataggc gtaaagcgtc gtccggctga ctccgagaaa
     9061 actcgacgcg tctttggcgg aaagccaagt gaccatgccg gaagattagc accccaacat
     9121 tgatcgtgca atcaatattg acaacgttga tggcggaatc tacgttgtgg tcatgagcaa
     9181 ccctatcatc cacaacggcc tcgagggaat tgtcgcatcc gaaaccgaac tgagcatggt
     9241 cgacggactg gccggcgagc tgatcatcgt cggctttccc gtcggcgagc tggccatgaa
     9301 cgctacgttc gaggagacgg ccttcctcct ctggtacggg cgcctgcccg acgcgaggga
     9361 gctggcggca tttcgcgcag atctggcctc gcggcgcgaa ctgcctcagg cagctctgga
     9421 tctccttcgg gcggcggcgg acaagcaagt agacacgatg gacgcgctgc ggatggccgc
     9481 gggcacgatc agcctgcgcg gatcttcgaa gaacgacgac gacgcggctc gcgaccttct
     9541 ggccgcattt cccacgatcg tcgcctccta ctggcgcctc cttcgcgctc acgatccc
//
LOCUS       DATLVW010000200         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_7416, whole genome shotgun sequence.
ACCESSION   DATLVW010000200 DATLVW010000000
VERSION     DATLVW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7416"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..830)
                     /locus_tag="VNN25_06500"
     CDS             complement(405..830)
                     /locus_tag="VNN25_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91212.1"
                     /translation="MIQRLEIYAIAIGAGLLLLVLGTTGGYFKGRLDEKAAITQKQLA
                     DDAKKMTDYQGTLEKRAAADDTARDKALAFVADIDKRMDGINAKFAKLPNVVVDARGC
                     ERLTDAARLRWNSVELLPTGNVDATSPIQPGGGETPRPQ"
     gene            complement(1051..1323)
                     /locus_tag="VNN25_06505"
     CDS             complement(1051..1323)
                     /locus_tag="VNN25_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91213.1"
                     /translation="MGTFRVEVQAVGGHGCQREVKAGGTVSDCGQPGCVDCISRRFVK
                     ALQDAGASFSTSGGIESVGYAKLTHWPHGHGTVNDDLLTGVRSGSF"
     gene            complement(1344..2033)
                     /locus_tag="VNN25_06510"
     CDS             complement(1344..2033)
                     /locus_tag="VNN25_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91214.1"
                     /translation="MNITADTLKAILSSPLALFLLMCLASIGNGVKQLAVIKQTGRPM
                     TCLEYWAHWPETLGTVISNALAFGLLLMTDQLNFASALSVGYGLNSLSDLIVRGGRSY
                     ALKSTPDDPNKKPPVAPSVDAAKILLLTLFIATVTSASIAIAAEVPIEKDAPKMEGHP
                     LTSVVVTQCNLIVAVYMTMPDGRLLRFDKSEAVPSDQLMTMAYSATRSERIEVSCNDT
                     GAAGYENHEPV"
     gene            complement(2030..2533)
                     /locus_tag="VNN25_06515"
     CDS             complement(2030..2533)
                     /locus_tag="VNN25_06515"
                     /inference="COORDINATES: protein motif:HMM:NF040141.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HXH91215.1"
                     /translation="MHYPLGSREPEVTMYLSLIRDTAASTYCLGVLHAGALEFQTIER
                     PWIDAPPGLGGHPETSCVPAGLYELVKHDSPKHPKSFALVNPKLDVWHLPEDVPADRR
                     PYARTAVLIHVANLASQLEGCVALGMGRGAGCVYSSALALDSFNLTVPWIVGHSLRID
                     FAEGIAP"
     gene            complement(2574..3038)
                     /locus_tag="VNN25_06520"
     CDS             complement(2574..3038)
                     /locus_tag="VNN25_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91216.1"
                     /translation="MPVPVEPDGPGWRIIAIGAWALVTTLFGIIANRLRTDLKEQGAR
                     IDLAYTRLESTVNKEDFRDALATFRAERDAQHKDNRDERIRLHEETLANFEKLSQKLD
                     SQAVQDQRILQLERDLAAVCKYAEDLKHMNVDPYVRATAVLESRVKALENDK"
     gene            complement(3043..3459)
                     /locus_tag="VNN25_06525"
     CDS             complement(3043..3459)
                     /locus_tag="VNN25_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91217.1"
                     /translation="MWHFYDLATGLFSGRSFSASDEAWLKDNLPDGHGVYEAARIDHL
                     SQRVNLISGEVVDYQPPQPSHDHEWNESAKRWQLSKTAVARIEALQRIQMLEAKQLRA
                     LREFALGYDGAKQRLQALDDEMAVLRRDVSEQKAPG"
     gene            complement(3465..>4613)
                     /locus_tag="VNN25_06530"
     CDS             complement(3465..>4613)
                     /locus_tag="VNN25_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91218.1"
                     /translation="KGALIFGTGAYTTATKFEIRYDGKFVTYYRDNVQWRSTPAPGLV
                     FFLDSSFDTPGAAADNVYFGALNPANSSPFVARGQCVVSDENVQKIGGVSAYDSDVYS
                     LEGYPTCHLTFKANQTNCFLAIGFSEAPAATQDFTNIKSGFILNSDGTYREVINGVGG
                     GASGTYTTKTVFALTYDGGTILPLVDGVSIDPHGDFTTQNRIYFLDSSFYTPGGGVNT
                     LQFGPGANLKLTDTSQLGSNAATNVGVSTVAGPTDFRVAPPNSQATVTFVNAVGPYTV
                     DADVIVTAQGYLDFKSSTSFSCQCLGDIQNQAEYIASSGHPYGGAILASAAAGVELQT
                     SFMLEKHYSLTRGAADSYNFQVEAGWSPANGGFAKFSVVMFKVEVIKR"
BASE COUNT          931 a         1420 c         1292 g          970 t
ORIGIN      
        1 gccatcgaat gccggtgcac ttggaaaccc tcctagcgcg ctcgtacagc gagcgcacaa
       61 acttgacatc gcaccaggga acccctttgc gccctcgcct aatttcataa cggcgttttg
      121 cctgctttcg cgcgttctcc aaacagcgat cccggtgctt tttctgatat ttccgctgcg
      181 cctcgcgcct cgcttcgcga gcctctatgg cagacagggg ttctttcgtt ggcttgtatg
      241 gatgcgcgcg cctcttcgca accaagttca ttgcgtttgt acgtttacgg ccatcttcgg
      301 tagcccgcca cgcaatctga tcttcccgaa ggcactcaac acaattcccg tgcgtatagc
      361 ggtggcaaat atgtccacgc acgcacggct tccccgtgaa atacttattg aggccgaggc
      421 gtttcgcctc cgcccggctg gatgggagaa gttgcatcga cgtttccggt tggaaggagt
      481 tctacggaat tccagcgtaa tctggccgca tcagtcaacc gctcgcagcc ccgagcatca
      541 acaaccacgt tcggtagttt cgcgaatttc gcattgatgc catccatcct cttgtcgata
      601 tcagccacga aggcgagcgc cttgtcccga gccgtgtcgt cggcggccgc cctcttttca
      661 agcgtgccct gatagtcggt catcttcttg gcgtcatcgg cgagttgctt ctgcgtgatg
      721 gcggccttct cgtccagacg tcctttgaag taaccgcctg tcgtaccgag cacgagcagc
      781 aggaggccgg cgccgatggc gatcgcgtaa atctcaagcc tttgaatcat ggcgctcccc
      841 tccgaatcca ctctgcgacc cgagtcagtg catccgccaa gcctgccggc gtcacgtcgt
      901 gatcgaaagg cttgcggacc atctcgttgt tccgagagat tgtggccgtg atgcgcagtt
      961 gctcgccggc tcggtcgtga acgatggcga cgagctcgac ctcgcgacgc ggagtcgtcc
     1021 gcgtcccgag atagggtttg tgtgcgccgc tcagaaactg cctgagcgaa cacccgtgag
     1081 gaggtcgtcg ttgaccgtcc catggccatg cggccaatga gtcaactttg cataaccgac
     1141 gctctcgata ccgccgctcg tgctaaacga cgcaccggca tcctgcagcg cttttacgaa
     1201 gcgccgcgaa atgcaatcga cgcagccggg ttggccgcaa tcgctcaccg tgccgccggc
     1261 cttgacctcg cgctggcagc cgtgcccgcc aaccgcctgc acctcgactc taaacgtacc
     1321 cataatgatc tcctgtcggt tgctcaaact ggctcgtggt tctcgtaccc tgccgcaccc
     1381 gtgtcgttgc acgacacctc gatccgctcg ctgcgggtgg ccgaataagc catggtcatc
     1441 agctggtcgg agggcaccgc ctccgacttg tcgaagcgca acagccttcc gtccggcatc
     1501 gtcatgtaga ccgcgacgat gaggttgcat tgggtcacga caacggacgt cagcggatgc
     1561 ccttccatct tcggcgcgtc tttctcgatg ggcacctcgg cggcgatcgc aatcgaagcg
     1621 ctcgtgacgg tggcgatgaa caacgtcagc aggaggatct tggccgcatc cacggacggc
     1681 gcaacgggtg gcttcttgtt cggatcatcc ggcgtgctct tcagggcgta ggagcgcccg
     1741 ccgcgcacga tcagatcaga gagcgaattg aggccatagc ccacactgag tgcgctcgca
     1801 aagttgagtt gatcggtcat cagcagcaag ccgaacgcca gtgcgttgct gatcacggtc
     1861 cctaaggtct cgggccaatg cgcccagtac tcgagacagg tcataggcct gccggtttgc
     1921 ttgatgacag cgagttgttt gaccccgttt ccgatggaag ccaggcacat cagcaggaac
     1981 agcgccagcg gcgaggacaa aatagctttg agggtgtcag ccgtgatgtt catggtgcga
     2041 taccctccgc gaaatctatt cgtagactgt gaccaacaat ccaaggaacg gtgagattga
     2101 aactgtcgag ggcgagcgcc gacgagtaca cgcagccggc gccgcggccc atacccagcg
     2161 caacgcatcc ctcgagctgg gaggcgaggt tcgcgacgtg gatcagcacc gccgtgcgcg
     2221 cgtacggccg acgatcagcc ggcacatctt ccggcaggtg ccagacatca agcttcggat
     2281 tgaccaaagc gaacgacttc ggatgcttcg gcgaatcgtg cttcacgagc tcgtaaagcc
     2341 ctgccggcac gcacgaggtc tccggatgac cgcccaggcc aggaggcgca tcgatccagg
     2401 gccgctcgat cgtctggaac tccagcgccc cggcgtgcag gacgcccagg caataggtcg
     2461 acgccgcggt atcgcggatc agggacaaat acatggtgac ctcgggctcg cgcgagccga
     2521 gtgggtagtg cacggagaaa ttcctgctga tccgccagac taagcgctgg agatcatttg
     2581 tcgttttcga gcgccttgac ccgagactct aaaacagccg ttgcacgcac gtagggatcg
     2641 acgttcatgt gtttgaggtc ttcggcgtat ttgcataccg ccgcgagatc cctctcaagt
     2701 tgcaaaatgc gctgatcttg cacggcctgc gaatccagct tctgcgaaag cttctcgaag
     2761 ttcgcgagtg tttcctcgtg cagtcgtatc cgctcatcgc gattgtcctt atgctgagcg
     2821 tcccgctccg cgcggaacgt ggcgagagcg tccctgaaat cctctttgtt gaccgtactt
     2881 tccaaccgcg tatatgcgag atcgatacgc gcgccctgct ccttcagatc ggttcgcagc
     2941 ctattagcga tgatgccgaa taaagtcgtc accaatgccc acgcgccgat cgcaataatc
     3001 cgccagcccg ggccgtcggg ctcaaccgga actggcatcg cgttacccag gtgccttctg
     3061 ttcggaaaca tcccggcgca gaactgccat ctcatcatcg agcgcctgca ggcgctgctt
     3121 cgcgccatca taacccagtg cgaactcgcg cagggcgcgg agctgtttgg cctccaacat
     3181 ctgtatcctc tgcagagctt caattcgggc cacggccgtc ttgctcaatt gccagcgctt
     3241 tgcgctctcg ttccactcgt gatcatgcga aggctgtggc ggctgataat ccacaacctc
     3301 gccgctgatc aagttcacgc gctgactgag atgatcgata cgcgcggcct catacacgcc
     3361 gtgaccatcc ggcaggttgt cttttaacca tgcctcatca gaagcgctga aactgcgccc
     3421 gctgaaaagc cccgtcgcca gatcatagaa atgccacatt ctgctcatcg cttgatcact
     3481 tccaccttga acatgacgac tgagaacttg gcaaaaccgc cgttggccgg ggaccatccg
     3541 gcctctacct ggaagttata actgtcggca gcaccgcgcg tgagagaata gtgcttctca
     3601 agcatgaacg aggtctgcag ttcaactccc gcagccgccg aggccaatat tgcgccaccg
     3661 taagggtggc ccgaactcgc tatgtattca gcttgattct ggatgtctcc gagacactga
     3721 caggaaaagg acgtactgga cttgaaatca agatatccct gcgctgtcac gatgacatcg
     3781 gcgtccacag tataagggcc taccgcattg acgaaagtga cggtcgcttg cgaatttggc
     3841 ggcgcaactc tgaaatccgt agggcccgca acagtgctaa caccgacgtt ggtggctgca
     3901 ttcgacccta gttgtgacgt atctgtcagc ttcaaattcg ctccaggacc aaactgaagc
     3961 gtgttcacgc cgccgcccgg agtataaaaa ctgctgtcca ggaagtagat gcgattctgt
     4021 gtcgtgaagt ctccgtgcgg atcaatgctc acaccgtcca ccagcggcag gatcgtaccg
     4081 ccgtcatagg ttagcgcaaa cacggttttc gtggtgtacg tgcccgaagc cccgccgccc
     4141 acgccattga tcacttcgcg ataggtgccg tcactgttga gaatgaagcc agacttgatg
     4201 ttggtgaagt cctgcgtcgc cgcgggtgct tcgctaaagc cgatggcgag aaagcaattc
     4261 gtctgattcg ctttgaaggt cagatgacag gtcggatagc cttccagcga atacacatcg
     4321 ctgtcatatg cggacacgcc tccgatcttc tggacgttct cgtcactgac gacgcactgt
     4381 ccgcgcgcga caaagggcga cgagttggct ggattcagcg ctccaaaata gacgttatcc
     4441 gcagcggcgc caggagtatc gaacgacgaa tccaggaaga acacgagtcc gggcgcgggc
     4501 gtgcttcgcc actgcacgtt atcgcgatag taggtaacga acttgccgtc atagcgaatc
     4561 tcgaacttcg tcgcggtcgt gtacgcgccg gtgccaaaaa ttaaggcgcc ttt
//
LOCUS       DATLVW010000201        11039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_503970, whole genome shotgun sequence.
ACCESSION   DATLVW010000201 DATLVW010000000
VERSION     DATLVW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503970"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..244
                     /locus_tag="VNN25_06535"
     CDS             110..244
                     /locus_tag="VNN25_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91219.1"
                     /translation="MGSSVSDEYPEVTLLLRHWSNGGSALPAVKKLADCGYCVRRRTA
                     "
     gene            341..610
                     /locus_tag="VNN25_06540"
     CDS             341..610
                     /locus_tag="VNN25_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91220.1"
                     /translation="MEAAIAQIANISDGWVRAIEEFANHPSEERWDELMRFVPVEEHY
                     QRFRYTLALLLSLHCDGNILFQCATKIGMTSDAFDLARSGRSTRK"
     gene            complement(909..1577)
                     /locus_tag="VNN25_06545"
     CDS             complement(909..1577)
                     /locus_tag="VNN25_06545"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXH91221.1"
                     /translation="MNLADHDEELATLFQSLEHSPDAVFVTDRTNHIVFWNEAARHML
                     GFTADEAIGGHCDRLLGGSDPFGNRYCTDECPIMRIANRGEIVRNFSLNLRTKESQHV
                     VTQVSVLQLRAGGPDDYYLVHMLRANGAQSEQRPASEPVSSSRPRLVAARESIDVRAR
                     KLTSREVEVLGMLAAGRTTPEIAERLHISQLTARSHIQNILEKLEVHSKVEAVAFAFQ
                     KNLI"
     gene            complement(1745..2137)
                     /locus_tag="VNN25_06550"
     CDS             complement(1745..2137)
                     /locus_tag="VNN25_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXH91222.1"
                     /translation="MRRRNLLIASVLLLVATSCRRANRPASAVAIPRPTQYELRLGKE
                     VYQSYCIGCHGDSGAGDGFNAFNLDPHPRDLSDPVFQKGKSNAELADAVRRGGAGVGL
                     TSLMPPWGHTLDDRHIDAVVLYIRSMRR"
     gene            complement(2134..4161)
                     /locus_tag="VNN25_06555"
     CDS             complement(2134..4161)
                     /locus_tag="VNN25_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXH91223.1"
                     /translation="MTLRRLFALSSLAFLIVLAFSPVKNALRPYRALQRQFRLVGIAR
                     ASSGKAAEAYSSRPIAIQQVWIPELNNRVDRCTTCHLGVVDAAMTTATEPFRTHPPTF
                     HTPRDVQRFGCTACHGGEGLATDQADAHGTSADATSPILPADLIEAGCGRCHDAEAVR
                     DAPTLSAGRALMTKSNCFACHKARGHEDFRADAPPLETLSMKTGAEWLRRWLSNPKAV
                     DANATMPNFQLTGDDIKVLSHYLFARPVPPQLQHAIELAGAEPNGDSARGKTLFAESR
                     CISCHTVGGKGNGSAPELGTIASRASRGWLIAFVRDPHAFYPQTKMPRYSFTDADVRD
                     IVAWFEDELKDFDAPANILTPLRVNQTFAEKGEKLFKSAGCFACHSGVKEGERFGPDL
                     YGIGDRKATSLDFGKLTDLPRTLAGWLDAKITTPRSFASGLKMPTFAFDAAQKRAIVT
                     ALLAMPATAVPDAYHSSPVQQAAIVPAGPVGEIMTRYRCLSCHQIGRAGGDISTAPLT
                     FEGSKVNHDWLVNYLAVPYSIRPIIAERMPVLKVSKEEAGRLADAIAMIYVDPSTPDD
                     PFLGRPASDHDAAEGERLYTTFGCRSCHIIGKIGGYYGPPLTETKARLKPGWVFRFLQ
                     NPQRWRADVRCPNYGMTDTDALRLTAYLETLAEAPAVPVAAAAPARKEAAK"
     gene            complement(4172..4873)
                     /locus_tag="VNN25_06560"
     CDS             complement(4172..4873)
                     /locus_tag="VNN25_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91224.1"
                     /translation="MTTPIEIIESDDPLANPWRVALVRPDGRPAVHETDERYVMTFPH
                     LILREIILFQVVVIVLSLAAILFNAPLESIANPVETPNPAKAPWYFLGLQELLHYFPP
                     VVAGVVLPALVGICLVVIPYWRVNLEGAPLWAAPSRWKSPAIAASVILLSVLFAAFSC
                     WPIVIPTVILGVAMFAAHAVRPGGRWQWLWGVTLPEWIMTWFVVIAVVLTVIGTFFRG
                     PGWMWVWPWMDAPRG"
     gene            complement(4870..5607)
                     /locus_tag="VNN25_06565"
     CDS             complement(4870..5607)
                     /locus_tag="VNN25_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXH91225.1"
                     /translation="MTVGEWVRNTSVYRSVIRHGVAPTNRNRSLIIFENLFLHVHPVK
                     VREKTLRFTHTFWLGGITLASTIILVATGILLMFYYHPSVPQAYRDMKELQYVVDNGV
                     FLRNLHRWSAHLMVFSAFLHMLRVFYHRAYRPPRQFNWVVGVVLLLLTLLLSYTGYLL
                     PWDQLAFWGVSVGTNMAKQAPLIGERVRFFLLGGNIVSENALLRFYVLHCVVLPVALL
                     LLISVHIWRVRKDGGVQGPPDDERRSP"
     gene            complement(5604..6101)
                     /locus_tag="VNN25_06570"
     CDS             complement(5604..6101)
                     /locus_tag="VNN25_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXH91226.1"
                     /translation="MPELEGMFGPEVEEEETLGRRDFFTQIGVGACAVVAVASGIVTL
                     DYLEPKVLFEPSTTYVAGSPLDFAPGVVVPNQEKKAFVIGGANGIYALSAVCTHLGCI
                     TRYKSDESAIACPCHGSRFDLEGNVTHGPAPRPLPWLDVSQDANGNLVVDTGIVIARG
                     KVFRP"
     gene            complement(6094..7929)
                     /locus_tag="VNN25_06575"
     CDS             complement(6094..7929)
                     /locus_tag="VNN25_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH91227.1"
                     /translation="MSGAEHNQEWLERNFPCKQACPVHTEAGKYVTLIAQGRFREAYA
                     VARRPNPLASICGRICAAPCEAVCRRGEIDAPISIRALKRFVTEKFGVESMLDFSVLT
                     EIYGDRTNRYPEKVAIIGSGPAGLACAHDLALLGYHATVFEAAPVAGGMLRLGVPEYR
                     LPRALIQLEINAILSLGVELKTNVRIGKDLTLSQLKAEGYAAIFLGVGSMKSRELAIK
                     GLELDGVLRGIDYLLNINLGYRVEMGKKVIVIGGGNVAVDVARTAARGGEQTNMQRNL
                     SIVQAMDVARSAVRFGARDVTVCCLESESEMPAAKDEVDEASIEGIRFAYRLGPKRIV
                     GANDVVTGVEFLRVSRVFDETGRFDPKYTAGSETVIEADTVIVAIGQTGDFAFLQPED
                     GVETRGGRIIIDPDSLATTAPGIFAGGDAAFGPRIAISAVADGKRAAKSIDTLLRGEP
                     RASDDASVDVVIEPRWDRELDFEGIPRQKPPSRPLARRIGIAEVEECFSEHAARREGS
                     RCLRCWTNTIFDQNAAAGTECILCGGCQDICPEHCIAIVPAARVISLPADAIELAPIL
                     LENRWVGSVMLKNEEVCIRCGLCAVRCPVGTISMRSFHRKESDVA"
     gene            complement(7933..8508)
                     /locus_tag="VNN25_06580"
     CDS             complement(7933..8508)
                     /locus_tag="VNN25_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH91228.1"
                     /translation="MKVVLASLIALMALPLMAGDIHGKVVAHSVRNSADAIVYVDKIA
                     GKTFPPPAAHAKIDQKNMQFTPHVLAVMAGTTVDFLNSDAVLHNVFSPDACADKFNLG
                     TWPQGQVKSFVFKKECFASLLCKVHSEMEGFVAVLPTPYFAVTAADGSYDIKDVPDGT
                     YTVKVWHPKLKAATKSVTVKGATEASFEIAK"
     gene            complement(8535..9926)
                     /locus_tag="VNN25_06585"
     CDS             complement(8535..9926)
                     /locus_tag="VNN25_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91229.1"
                     /translation="MSMHRGRISLVCFLLTVLIFVLTAPSASAIPAFARKYETSCQTC
                     HTGFPKLNPFGEAFRLNGYRMPKETEEQIKIKPVSLGAEAYKKMWPNAIYPSDLPGQV
                     PLALNVKMASVYASSLDDTGHAIVHNDFQFPQEANLFAGGTLGEHMSFLGEVTWSENP
                     DGSSSTEIERMHLQAGSFIGPEHLFNIKIGKFAADLDYGFHEMWLSTDNGVDNLFSFN
                     PIGLHGGTGISEGAISLPVNVKGIEAYGVAKHRWFYTVGVVNGLGPSASGNVDGNASK
                     DVYARVDYKFGGMGLDGDSTGVTLPPENWRERSFRVGVLGYHGNGSGVDFPITDETGA
                     TVNVQDRTFNRAGLFASWYYDDLNVFGVAMSGWDRLGTFAADGSLSSAQSFRYNSWFV
                     QSDYVILPPLQASLRYESVTPPGAAGVKSLRALNASMAYFFYANVKTMVEYRRDLSES
                     KNYQLSTILRFAF"
     gene            10058..10531
                     /locus_tag="VNN25_06590"
     CDS             10058..10531
                     /locus_tag="VNN25_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXH91230.1"
                     /translation="MTKSIRFHLNGRPVALDTDERRTLLWVIRTDLNLTGTKFGCGEG
                     LCGACTVAVNGTAVRSCQTSLADVENKNVVTVEGLEHDGKLHPLQQAFIEHGGFQCGY
                     CTSGMLMNAWAFLQKQPHPSRSAIVDEMNGNLCRCGAHQGIIAAIEDASKAGGAR"
     gene            10528..>11039
                     /locus_tag="VNN25_06595"
     CDS             10528..>11039
                     /locus_tag="VNN25_06595"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH91231.1"
                     /translation="MTTQPWPLPPAESSSPGIATGIDRRLFCKLLGGGIVVLMTSKPA
                     DLFAQRRGYPEDLNAYLRIDENGGVTLFSGKIEMGQGVHTSLAQMAAEELGVSLDSIT
                     MVMGDTDLCPWDAGTWGSQSTRMFGPAVRAAAAEARAVLMKLASEKLGSPKEQLVVEN
                     GVVSNGSKKVT"
BASE COUNT         2022 a         3458 c         3627 g         1932 t
ORIGIN      
        1 gcaaggatat cgagcaccag acgggcaagc cggtggtctc ggccgcgaac ttcaagcggc
       61 ttggcagcag gcggccgaag agactgaagg ccggagagga ttgacccgga tgggcagctc
      121 cgtcagcgac gagtaccccg aggtgacgct cctgctgcgc cactggagca acggcggaag
      181 cgcgttgccg gctgtgaaaa agctggccga ctgcggctac tgcgtccgga gaagaactgc
      241 ttgagcgcca ggatctgctt tcgatagcga agtcggggac tacgcggcgg ccgtcgcccg
      301 cctggcgttg cgagcgccgg attgcgccga tcgcgaaagg ttggaagcgg ccatcgcgca
      361 gatcgcgaac atatccgacg gctgggtgag agcgatcgag gagtttgcga atcatccgag
      421 cgaagagcgc tgggacgagc tgatgcgctt cgttcccgtc gaagagcact accagcgctt
      481 ccgttacact ctggcccttc tcctctccct tcattgcgat ggcaacattc tctttcagtg
      541 cgccacgaag atcggaatga cctcggacgc attcgatctc gcccgaagcg gcaggtcgac
      601 ccggaagtga tcgaagcgcg aggtgaagga tcgccggcgc gtcccgcctg gctggtctcg
      661 ccgcgcaggc cgccttcgcc cgcaacgacc ggtggtcgac ccttcgctat ctccgcgaag
      721 caagccaata cggagagagc tccgttctgt catgggccag catctccgaa atccggcgcg
      781 aggctgatga ggagctcaac gatgaattgg accgcgtcgg cctgccgcgc gtgtagagcc
      841 cacagagtaa cgatgaagct acttgttacg gcgcgcgcag cctgacgaac gatgaaccag
      901 gagccgcctc aaatcaggtt cttctgaaac gcaaaagcca ccgcttccac tttggagtgc
      961 acttcgagct tttccaggat gttctggatg tggctgcgcg cggtgagctg ggagatgtgc
     1021 aggcgctcgg cgatctcggg agtggtgcgg ccggcggcga gcattcccag cacctcgact
     1081 tcacggctgg tcagtttgcg cgcacggaca tcgatcgatt cgcgcgcggc cacgagccgc
     1141 ggacgggaag agctcaccgg ttcgctggcc gggcgttgct cggactgcgc cccgttcgcg
     1201 cgaagcatgt gcaccaggta gtagtcgtca ggaccgccgg cacgaagctg gaggacgctc
     1261 acttgtgtga ccacgtgctg gctctccttc gtgcgcagat tgagcgaaaa attgcgcacg
     1321 atctcgccgc ggttggcgat gcgcatgatc ggacactcgt cggtgcaata gcggttgccg
     1381 aatgggtcgc tgccgccgag tagacgatcg cagtgaccgc cgatggcttc gtcggcggtg
     1441 aacccgagca tgtggcgcgc ggcttcgttc cagaagacga tgtggttcgt acgatccgtc
     1501 acgaagacgg catcgggcga atgttcgagc gactgaaaga gtgtcgccag ttcttcatca
     1561 tggtctgcca ggttcaaacg gcgccgccag tctacgaccg tgacgaatat cgccgcggat
     1621 cgatcgcgcc cggaaggtgt ggggtgaaat gctgggggct gggcagccga cccccccacg
     1681 gtcgagcgaa ggaagtatag acctttcgga accgcggcgg ctccgacccc cgactcccgg
     1741 ctccctacct ccgcatcgat cggatgtaca ggacaaccgc atcgatgtgc cggtcgtcga
     1801 gggtgtgacc ccacggcggc atgaggctcg tcagtccgac accggcgccg ccgcggcgga
     1861 cggcatcggc gagctcggcg ttcgacttcc ccttctgaaa cacgggatcg ctcaggtcgc
     1921 gcgggtgcgg atcgagattg aacgcgttga acccatcgcc ggcgccgctg tcgccgtggc
     1981 atccgatgca gtagctctga tagacctcct tgcccagccg caactcgtac tgcgtcggcc
     2041 gcgggatggc gaccgcggag gcggggcgat tcgcgcgccg gcacgacgtc gccaccagca
     2101 ggaggacgct cgcgatcagc agattgcgtc gtctcatttc gcggcctcct tccgcgccgg
     2161 cgcggccgcc gccacaggca ccgccggcgc ctcggccagc gtctcgagat acgcggtcag
     2221 acgcagcgcg tcggtgtcgg tcatgccgta gttgggacag cggacgtcgg cgcgccagcg
     2281 ctgcgggttc tgcaggaagc ggaacaccca gcccggcttc agccgcgctt tcgtctcggt
     2341 caacggcggc ccgtaatacc ctccgatctt gccgatgatg tgacaggagc ggcagccaaa
     2401 cgtcgtgtaa agccgttctc cctcggcggc atcatgatcg gatgcgggcc ggcccaggaa
     2461 cggatcatca ggcgtcgatg gatcgacgta gatcattgcg atcgcatcag cgagccgtcc
     2521 ggcctcttct ttcgagacct tcaggaccgg catccgctcg gcgatgatgg gccggatcga
     2581 atacggaaca gccagatagt tgaccagcca gtcgtgattg accttgctcc cctcgaacgt
     2641 cagaggtgcc gtcgagatgt cgccgccggc tctgccgatc tgatggcagg agaggcagcg
     2701 atagcgggtc atgatctcgc cgacgggccc ggccggaacg atggcggcct gctgcaccgg
     2761 cgacgagtga taggcgtccg gcacggcagt ggcgggcatg gccagcagcg ccgtgacgat
     2821 ggcgcgcttc tgcgcggcgt cgaaggcgaa ggtgggcatc ttcagacccg aggcgaagga
     2881 ccgtggcgtg gtgatcttcg catcgagcca acccgcgagc gttcgcggca ggtccgtgag
     2941 cttgccgaag tcgagcgatg tggccttccg gtcgccaatg ccgtagagat cggggccgaa
     3001 tcgctcgcct tccttcacac cgctgtggca tgcgaagcag cctgcgctct tgaacagctt
     3061 ctcccctttt tctgcgaacg tctgattgac gcgcaggggc gtgaggatgt tcgcgggcgc
     3121 gtcgaagtct ttcaactcgt cctcgaacca ggccacgatg tcgcgcacat ccgcgtccgt
     3181 gaacgagtac cgcggcatct tcgtctgcgg gtagaacgcg tgcggatcgc gaacgaaggc
     3241 gatcagccat ccgcgcgaag cgcgcgacgc gatcgtcccc agttccggag cggaaccgtt
     3301 ccccttgcca ccgaccgtgt gacaggagat gcatcgtgac tcggcgaata gcgtcttgcc
     3361 gcgggcggag tcgccgttcg gctccgcccc cgccagctcg atggcgtgct gcaattgcgg
     3421 cgggacggga cgcgcgaaga ggtagtgcga cagaaccttg atgtcgtcgc cggtgagctg
     3481 gaagttcggc atcgtggcgt tggcatcgac cgccttcgga ttgctgagcc agcgccgcag
     3541 ccattcggcg ccagtcttca tcgacagcgt ctcgagtggc ggggcgtcgg cgcggaagtc
     3601 ttcgtggccg cgggctttgt ggcaggcgaa gcagttcgac ttcgtcatca gcgcgcggcc
     3661 ggccgagagc gtcggcgcgt cgcgaaccgc ctcggcgtcg tggcagcgcc cgcatcccgc
     3721 ctcgatgaga tccgcgggaa ggatcggaga ggtcgcgtca gccgaggtgc cgtgtgcgtc
     3781 ggcttgatcg gtggccagcc cctcgccgcc atggcaagcc gtacatccga aacgctgcac
     3841 gtcacgcggc gtgtggaagg tcggcgggtg cgtgcggaac ggctccgtcg cagtggtcat
     3901 ggcggcgtcc acaacgccga ggtgacaggt cgtgcatcga tcgacgcgat tgttcagttc
     3961 cggaatccag acctgctgga tcgcgatcgg acgggacgaa tatgcctccg ccgctttccc
     4021 gctcgacgcc ctggcaatcc ccacgaggcg aaactggcgc tgcaacgcgc ggtaaggccg
     4081 cagcgcgttc ttgaccggcg agaaggccag cacgatgagg aacgcgaggc tggaaagggc
     4141 gaagagtcgt cgcagcgtca catcggcctc ctcatcctcg gggtgcgtcc atccacggcc
     4201 agacccacat ccatcccggc ccgcggaaga acgttccgat cacggtcagc accactgcga
     4261 tcacgacgaa ccacgtcatg atccattccg gcagcgtaac gccccacaac cactgccagc
     4321 ggccgcccgg acgcacggca tgggccgcga acatggccac gccgaggatg acggtcggta
     4381 tgacgatcgg ccagcaactg aacgcggcga agaggaccga aagcaggatg accgacgcag
     4441 cgatcgcagg agacttccat cgcgacggtg cggcccacag cggcgcgcct tcgagattga
     4501 cgcgccagta cggaatgacc acaagacaga tgccgaccag cgccggcaac accactccgg
     4561 ccacgaccgg cggaaagtag tgcagcaact cctgaagacc gaggaagtac cacggcgctt
     4621 tggccggatt cggcgtctcc accggattgg cgatgctctc cagcggcgcg ttgaaaagga
     4681 tcgccgcgag ggagagaacg atcactacga cctgaaagag gatgatctcg cgcaggatca
     4741 ggtgcggaaa cgtcatcacg tagcgctcgt cggtctcgtg aactgccgga cgcccgtccg
     4801 gacgcacgag cgcaacgcgc cacggattgg cgagcggatc gtccgattcg atgatctcga
     4861 tcggtgtggt cacggcgatc tcctctcgtc gtcaggcggt ccctgcactc ccccatcctt
     4921 ccgcacacgc cagatgtgaa cggagatgag gagcaggagc gccaccggca ggacgacgca
     4981 atgcagcacg tagaaccgga gcagcgcgtt ctccgagacg atgttgccgc cgaggaggaa
     5041 gaagcggacg cgctcgccga tcagcggcgc ctgcttggcc atgttcgttc cgaccgagac
     5101 gccccagaac gcgagctggt cccacggcag caggtagccg gtatacgaca gcaacaacgt
     5161 caggagcagc aggaccacgc cgacaaccca gttgaactgg cggggcggcc ggtaggcgcg
     5221 gtgatagaag acgcggagca tgtgcaggaa cgcgctgaag accatcagat gcgccgacca
     5281 gcggtggagg ttgcgcagga agacgccgtt gtcgacgacg tactgcagtt ccttcatgtc
     5341 gcgatacgcc tgcggcacgg acgggtggta atagaacatc aggaggatgc ccgtggccac
     5401 gaggatgatc gtgctggcca gcgtgatgcc gccgagccag aacgtgtgcg tgaagcgaag
     5461 cgtcttctcc cgcaccttca ccggatgaac gtgaaggaag aggttctcga agatgatcag
     5521 agaccggttg cgattcgtcg gcgcgacgcc gtgccggatc accgaccggt agacagatgt
     5581 gttgcgaacc cactccccga cggtcatggc cggaacacct ttccgcgcgc gatgacgatg
     5641 ccggtgtcga cgacgagatt gccattcgca tcctggctga cgtcgagcca cggcagcggg
     5701 cgcggcgccg gtccgtgtgt aacgttccct tcaagatcga agcggctgcc atggcagggg
     5761 caggcaatcg cgctttcatc ggacttgtag cgggtgatgc agccgaggtg cgtgcagacg
     5821 gcggagagtg catagatgcc gttcgccccg ccaatcacga acgctttctt ctcctgattg
     5881 ggaacgacca cgccgggcgc gaagtcgagc ggcgagccgg ctacgtacgt tgtcgacggc
     5941 tcgaagagca ccttcggctc gagatagtcg agcgtcacga tgccggaagc gacggcaacg
     6001 acggcgcagg cgccgacgcc gatctgcgtg aagaagtcgc ggcggcccag cgtctcctcc
     6061 tcctcaacct cgggaccgaa cattccttcc agttcaggca acgtcgctct cctttcggtg
     6121 gaagctgcgc atgctgatcg tgccgacggg acaacgcacc gcgcagagtc cgcagcgaat
     6181 gcagacctct tcgttcttga gcatgaccga gcccacccac cggttctcga gaaggattgg
     6241 cgcgagctcg atcgcgtcgg ctgggagcga gatcacgcgc gcggcgggga cgatggcgat
     6301 gcaatgctcc ggacagatgt cctggcatcc gccgcagagg atgcactccg ttcccgcggc
     6361 ggcgttctga tcgaagatgg tgttggtcca gcagcggagg cagcggcttc cctcgcgccg
     6421 cgccgcgtgc tcgctgaagc actcctcgac ctcggcgatg ccgatccgtc gcgccagcgg
     6481 ccgggatgga ggcttctgcc gcggaatccc ttcgaagtcg agctcgcgat cccagcgcgg
     6541 ctcgatgacg acatccacgg atgcgtcgtc gctggcccga ggctctccgc gcagcagcgt
     6601 gtcgatcgat ttcgcggcgc gcttgccgtc cgccaccgcg ctgatggcga tgcgcggtcc
     6661 gaacgcggca tcgccgccgg cgaagatgcc cggcgcggtg gtggccagcg agtcggggtc
     6721 gatgatgatc cgcccgccgc gcgtctccac gccgtcctcc ggctgcagga aggcgaagtc
     6781 gccggtctgg ccgatagcca cgatcacggt atcggcctcg atgaccgtct ccgatccagc
     6841 ggtgtacttc ggatcgaagc gcccggtctc gtcgaagacc cgcgacacgc gcagaaattc
     6901 gacgcccgtc accacgtcat tcgcgccgac gatccgtttg ggaccgagcc ggtaggcgaa
     6961 gcggattcct tcgatcgagg cctcgtccac ttcatccttc gcggcgggca tctcgctctc
     7021 cgactccagg cagcagacgg tcacgtcgcg cgcgccgaag cggaccgcgc tacgcgccac
     7081 atccatggcc tgcacgatcg agaggttgcg ttgcatgttc gtctgctcgc ctccgcgtgc
     7141 tgccgtgcgg gccacgtcga cggctacgtt gccgcctccg atcacgatga ccttcttccc
     7201 catctcgacg cggtagccga ggttgatgtt gaggaggtag tcgatgccac gaagtactcc
     7261 gtcgagctcg agccccttga tggctagttc gcggctcttc atcgagccga cgccgaggaa
     7321 gatggccgca tacccttcgg ccttcagttg cgagagcgtc aggtctttcc cgatccggac
     7381 gttcgtcttc agctccacgc cgagcgagag gatggcgttg atctcgagct ggatcagcgc
     7441 gcgcggcagg cggtattcgg gaacgccgag gcgcagcatc ccgcccgcta caggagcggc
     7501 ttcgaagacc gtggcgtggt acccgaggag cgccagatcg tgcgcgcagg ccaggccggc
     7561 ggggccggag ccgatgatgg ctaccttctc cggatagcga ttcgttcggt cgccgtagat
     7621 ttcggtgagc accgagaagt cgagcatgct ctccacgccg aacttctcgg tgacgaaccg
     7681 cttgagcgcg cgaatcgaga tcggtgcgtc gatctcaccc cggcggcaga ccgcctcgca
     7741 gggagcggcg cagatgcgcc cacagatcga cgcaaggggg ttcggtctgc gggccaccgc
     7801 gtacgcctcg cggaagcgcc cttgcgcaat cagggtcacg tacttccccg cttccgtatg
     7861 aacaggacag gcctgtttgc acgggaagtt acgctccagc cactcctgat tgtgctcggc
     7921 gccgctcatg gctcacttcg cgatctcgaa gctcgcctcg gtcgcgcctt tcacggtgac
     7981 gctcttggtg gccgccttca gcttcgggtg ccagaccttt acggtgtacg tcccgtcggg
     8041 aacgtctttg atgtcgtacg agccgtcggc cgccgtcacg gcgaagtacg gcgtcggtag
     8101 cacggccacg aagccttcca tctccgaatg gactttgcac aggagcgacg cgaagcattc
     8161 cttcttgaag acgaagctct tcacctgtcc ctgaggccac gtgcccaggt tgaacttatc
     8221 ggcacaggcg tcgggcgaga agacgttgtg gaggacggcg tcagagttga ggaagtcaac
     8281 cgtcgtgccg gccatgactg caagcacgtg cggcgtgaac tgcatgtttt tctgatcgat
     8341 cttggcgtgc gccgctggcg gcgggaacgt ctttccggcg atcttgtcga cgtagacgat
     8401 ggcgtcggcg gagttgcgaa cgctgtgcgc gacgactttg ccgtggatgt cgccggccat
     8461 gagtggcagc gccatgagtg cgatgagcga tgcgaggaca actttcattg aatatctcct
     8521 tgttggtaat ggggtcagaa ggcgaagcgg aggatggtgg agagctggta gttcttcgac
     8581 tcgctcaggt cgcgccggta ctcgaccatc gttttcacat tggcgtagaa gaagtaggcc
     8641 atgctggcgt tgagcgcgcg aagagacttg actcccgccg cgcccggcgg cgtcacgctt
     8701 tcataccgaa gcgatgcctg aagcggcggc aggatcacgt agtccgattg aacgaaccag
     8761 gagttgtagc ggaagctctg tgcactggag agtgatccgt ccgccgcgaa tgtgccgagg
     8821 cgatcccacc cgctcatggc cacgccgaag acattgagat cgtcgtagta ccaggaggcg
     8881 aagagcccgg cgcggttgaa ggttcggtct tgcacattca cggtggcccc ggtctcgtcg
     8941 gtgattggga agtcgacccc ggaaccgttg ccgtgatacc cgagaacacc gacgcggaac
     9001 gaccgctctc tccagttctc cggcggcagc gtcactcccg tagaatcgcc gtcgagcccc
     9061 attccgccga acttgtaatc gacacgcgcg tagacgtcct tgctggcgtt gccgtcgaca
     9121 ttaccgctcg cggaagggcc gaggccgttg acgacgccca cggtgtagaa ccagcggtgc
     9181 ttggctactc cgtacgcctc gattcctttc acgttcaccg gaaggctgat ggccccctcg
     9241 gagatccccg tccctccatg caatccgatg ggattgaagg agaagagatt gtcgacgccg
     9301 ttatcggtcg acaaccacat ctcgtggaat ccgtagtcga ggtcggccgc gaacttgccg
     9361 atcttgatgt tgaacaggtg ctcggggccg atgaacgatc ccgcctgcag atgcatgcgc
     9421 tcgatctcgg tggaactgct gccgtccggg ttttcggacc atgtgacctc gccgaggaag
     9481 ctcatgtgct cgccgagagt gccgcccgcg aagaggttcg cttcctgcgg aaattgaaag
     9541 tcgttgtgca cgatcgcatg accggtgtca tcgagtgacg acgcgtagac gctggccatc
     9601 ttcacgttca gcgcaagcgg cacctgtccg ggcagatcgc tgggatagat cgcattcggc
     9661 cacatctttt tgtacgcctc ggcgccgagg ctgaccggct tgatcttgat ctgctcttcg
     9721 gtctccttcg gcatccgata gccgttgagc ctgaatgcct ctccgaatgg attcagcttc
     9781 ggaaagccgg tgtggcaggt ctggcaggat gtctcgtact tgcgagcaaa cgccggaatc
     9841 gcactcgctg atggtgcggt gaggacgaag atcagtaccg tcaacagaaa acacacgagg
     9901 ctgatgcgcc cgcgatgcat gctcatgact tctccttttg catgcaccga cagtgatctt
     9961 ccgcgccacg aagctccatc ccccaaccgc agggaatcgc gtcatgtgtt tgggggaggc
    10021 gtgcgatacc atggaccccc taacgcagga ggctcgcgtg acgaagagca tccggttcca
    10081 cctgaacggc cggcccgtgg cactcgacac ggacgaacgg cgcacactgc tgtgggtcat
    10141 ccgcaccgat ctcaatctca ccggcacgaa attcggctgt ggcgagggct tgtgcggcgc
    10201 ctgcaccgtc gccgtcaacg gcactgcggt tcggtcctgt cagacctcgc tggccgatgt
    10261 cgaaaacaag aacgtcgtta ccgtcgaagg actcgagcac gacgggaagc tgcatccgct
    10321 gcaacaggcc ttcatcgaac acggtgggtt ccagtgcggc tactgcacct cagggatgct
    10381 catgaacgcg tgggcctttc tgcagaagca gccacaccct tcgcgcagcg cgatcgtcga
    10441 tgagatgaac gggaacctct gccgctgcgg cgcgcatcag ggaatcatcg ccgcgatcga
    10501 agacgcgtcg aaggccggag gtgcccgatg acgacgcagc cgtggccact ccctccggca
    10561 gaatcatctt cgcccggaat cgctaccggc atcgaccgcc gccttttctg caagctcctc
    10621 ggcggcggca tcgtcgtgct gatgacgtcg aagccggccg atttgttcgc gcagcggcgc
    10681 ggctatcccg aggatctcaa cgcctacctg cggatcgacg agaacggagg cgtgaccctc
    10741 ttctccggaa aaatcgagat gggtcagggc gtacacacat cgctggcgca gatggcggcc
    10801 gaggagctcg gcgtctcgct cgattcgatc accatggtca tgggcgacac cgatctctgt
    10861 ccgtgggatg ccggcacgtg ggggtcgcag tcgacgcgga tgttcggccc ggcagtgcgc
    10921 gcagcggcag ccgaggcgcg agcggtgctg atgaagctgg cgtcagagaa gctcgggagc
    10981 ccgaaagagc agctcgtcgt cgagaacgga gtggtgtcga acggttcgaa gaaggtcac
//
LOCUS       DATLVW010000202         6209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_343148, whole genome shotgun sequence.
ACCESSION   DATLVW010000202 DATLVW010000000
VERSION     DATLVW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343148"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VNN25_06600"
     CDS             <1..1276
                     /locus_tag="VNN25_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91232.1"
                     /translation="DTAMSSYPNGIQSLNGKVFFTASDGVASSVWTSDGTEAGTLPLA
                     ASTAGGSRPFGQGIVSGGVYYYVAVAAAFVQELWRTDGTAAGTFRLTQQSDLRNFDAL
                     IPFKGGLFFQADDAAHGVEPWFTDGTVAGTRMIADILPGTSGSFLYAKSSIVAGDYVY
                     FSAQGSGMDRAQPWRTDGTAAGTQSLSAPDGQDSESPQAFTRVGDWVFYASALNDNAF
                     NLWKTNIASGESILVHRFVNVSAPVMMWNVGGTLLFMIDGELWRSDGTGAGTMKVRDV
                     IPVFFCVRPGDTAVGDGVLFWFGYNNFTPELWRSDGTTAGTFRLGTFEGALGDTLEMC
                     VPHPLLYHGGRLYFTGNDDIHRAELWVSDGTIAGTHLLYDLNPGVAPSDPDQFMMAGG
                     TLYFAATGANTGHELWEACIDDCPVRRRASGR"
     gene            1315..1740
                     /locus_tag="VNN25_06605"
     CDS             1315..1740
                     /locus_tag="VNN25_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXH91233.1"
                     /translation="MKQIIHAVHIHAAPAAVFEALTTGKGLRGWWTKKVDAEERQGGI
                     IRFTFAGDFNPQMEQASIEKDRAVRWRCVGGHANWQDNTFTFSLAERNGETMLMFEQG
                     YARELSDEVYGIYNFNWGYYLNSLKLLCEKGVGVPFSPG"
     gene            2198..4537
                     /locus_tag="VNN25_06610"
     CDS             2198..4537
                     /locus_tag="VNN25_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXH91234.1"
                     /translation="MSFRASLLTVFLFVLALPLLGADPAHARADYDQLQRWQFTVEPI
                     AITEPLTIGRDVAMIKLLSGKVHLMQPVSGRTTGLVFEGEGRFTMAVPDTIERAQLRR
                     FSRHDISSVDVRFTQLVLRVSDDTIDKLFPGPQAQWAKNALAENRENHWLIDLGRDID
                     AKVIAAMLNPGALQMTAAIKTPDYDWLTFDYDSTRREEIQLIRFDRQTEEVWISLDRA
                     KDRDPSGGHGESDVRPATISSLDIKADLSKGKWNSRRVGETDQAMLNGHYTIAEVITP
                     NIDGIGALAMELYPTAKNVTAIDDKGQPLTILRDNIGARSSGISNDVSDSTMTVIFPA
                     PLRANASRVVTFQYDLETPNYAPGNAWYPTIPDAFDATTAHIELKVSKRNEVRSMGKR
                     ISETEAADGTKTSVWLVEKPAKMVTFSTAERFVEVPLELPGAPKVISFGTVTGLDPGN
                     RIRNAGADVINSLRFYQLLFDDKLDTPQIYATAIAAGHGQAFDGFLHLSEFSYEEHPG
                     ATELFRTHEVAHEWWGHKVGWKTYRDQWLSESFAEYSAMMFVQATVKGGDKYYDEIVS
                     VYDSIVQGNMGGGFSKFNRPWLVEFNPSARARIGPIGVGYRAATAEVPAGYEIQAYFK
                     GPLVLHMLRTMLRFKTGHDDVFFKILRDFIKDYSGKDASTADFQKEVEKNAPGDWSFF
                     FNSWIYDSAIPTIRWSYKVEPTATGGSKISVTVKRSGVGDDFVMVAPVRLEFDGNRAG
                     MFYVLINKNEQTVTHELSSTPRNVIFAPDHSLLANIRRE"
     gene            4575..5144
                     /locus_tag="VNN25_06615"
     CDS             4575..5144
                     /locus_tag="VNN25_06615"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH91235.1"
                     /translation="MRLTSIVAMAIIAASPLAAAVRSARAPHEMRQTTPPAIPKSGVR
                     AELLHDLDDMQKKILSLAAAVPASKYDWRPAHGVRSVSEVYMHMAGGNYTLVSFVGDH
                     PMLDTRSFERITEKPRIIEELRKSFDYLRDAIINTSDADLDKSIHMFGNDSTERAAFM
                     LALNHLHEHLGQSVAYARMNGIVPPWSTE"
     gene            complement(5240..>6209)
                     /locus_tag="VNN25_06620"
     CDS             complement(5240..>6209)
                     /locus_tag="VNN25_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXH91236.1"
                     /translation="CMGGVNNALAWGVGATEYANLVHSGFTQVEVSESIRFELVGTLR
                     ENVTAKDVMLYILLNYAKPQMTLDRIMEFTGPGLRSLSMDERATLANMATECAARTAI
                     IEADEKTFAWIAAMRPEADIEALRARAVAPDPDAVYAGGVHTIDLSEIRPMVAHPGDP
                     DHGVPSDPTNGAFVDEVSDVKIDIAYAGSCTAGKVDDFLFYHQVAKEAVDAGLKVAEG
                     VKFYIQFGSQAVETFARENGLLDTFERAGAIVLNPGCGACIGCGPGVSEDSDQVTISA
                     INRNYKGRSGPGKLWLASPLTVAASAFTGHVTAYTPGMFAKELVEA"
BASE COUNT         1233 a         1928 c         1877 g         1171 t
ORIGIN      
        1 cgatactgct atgagctcgt accctaacgg aatccagagt ctcaatggaa aggtcttctt
       61 cacggcatcg gacggagttg cttcttctgt ctggaccagt gacggcacgg aagccgggac
      121 gttgccgctg gctgcctcga ccgcgggagg atcgcggcca tttgggcagg gcatcgtcag
      181 cggtggcgtc tactattacg tcgcagtcgc ggcggctttc gtgcaagagc tgtggcgcac
      241 cgacggtacg gccgccggaa ccttccgcct tacgcagcag tcggacctcc ggaactttga
      301 tgcattgatt ccgttcaagg gcggcctgtt ctttcaggca gacgacgcag cgcacggcgt
      361 ggagccctgg ttcaccgacg gcacggtcgc cggaacccgg atgattgccg acatcctgcc
      421 cggaacaagc ggctcgttcc tttatgcgaa gagctcgatc gtcgccggcg attatgtgta
      481 tttctccgca caaggcagcg gaatggatag ggcgcagccc tggcgaacgg acgggaccgc
      541 agcgggaaca cagtcgctca gcgcgccgga cgggcaggac tccgaatcgc cgcaggcatt
      601 cacgcgcgtc ggtgactggg tcttctacgc ttctgctctt aatgacaatg cgttcaatct
      661 gtggaagacg aacatcgcct cgggcgagag catcctggtc caccggtttg tcaatgtgag
      721 tgcacccgtg atgatgtgga acgtcggcgg tacgctgctg ttcatgattg acggagagct
      781 gtggcgcagt gacggcaccg gggccggaac gatgaaggtt cgcgacgtaa ttccggtctt
      841 cttctgcgtg aggcccggcg acacggccgt tggcgatggc gttcttttct ggttcgggta
      901 caacaacttc acccccgagc tctggcgcag cgacggaacc acggcaggca cgttccgtct
      961 gggcacgttc gaaggggctc ttggcgacac gctcgaaatg tgtgtgcctc acccgctcct
     1021 ctaccacggc ggacgactct acttcacggg caacgacgac atccaccgcg ccgagctttg
     1081 ggtctccgac ggcacgatcg ccggaaccca tttgctgtac gacctcaatc cgggagtcgc
     1141 cccgtcagac cccgatcagt tcatgatggc aggcggcact ctctacttcg cggccacggg
     1201 cgcgaacacg ggacacgagt tgtgggaggc ctgcatcgac gactgtcctg tacggcgtcg
     1261 tgcgtcggga agatagcggc gagcgagccc ccggattgga gtgttgcaca tcacatgaaa
     1321 cagatcattc atgcggttca cattcatgcg gcgccagcag ctgtctttga ggcgctcacc
     1381 accggcaagg gcttgcgcgg atggtggacg aagaaggttg atgccgagga gcggcagggt
     1441 ggcatcatcc gattcacgtt tgccggagat ttcaatccac agatggagca agcctcgatc
     1501 gagaaggatc gtgcggttcg gtggcgatgc gttggcggcc atgcgaactg gcaggacaat
     1561 acattcacgt tctccctcgc ggagcgcaat ggcgaaacga tgctgatgtt cgagcagggc
     1621 tacgcgcgcg agctgtcgga tgaggtatac gggatctaca acttcaactg gggctactac
     1681 ctcaatagcc tgaagttgct gtgcgagaaa ggcgttggcg ttccgtttag tccgggatga
     1741 gcgagagtca aaaccggtca cgcggagacg cgggggacac ggagaagatc tcggctttcg
     1801 atcaggagtg tttcggatgg gcgatcccta ccctctcgcg atgacaagcc gcgcagactc
     1861 tcgatgacgc cgaagacctg acactccacg cgacgtagag cacgaagttc cccttcgctc
     1921 ggacgggagc gtcagatggg attcttcgga gtcggcggag gaggaggggc cgggcccttc
     1981 ggcgggcctt tcggaggcgc gggactggca gtccttctca tgtcgccgta ctcgccgacg
     2041 agattacggg aaaatacagt gcagtggcga cagtgacagc tgttactcgg cactgtgtct
     2101 cctttccgcg ggattccgcg gttgttcctt taagatgttc tcattcatcc tcaatcagtc
     2161 aagctgtgtt cttcatgggg tggaaccagg gggctccttg tcttttcgcg catcgttgtt
     2221 gacggtcttt ctcttcgttc tcgccctacc ccttctggga gccgatccgg cgcatgctcg
     2281 cgctgactat gaccaattgc agaggtggca attcacggtc gagccgatcg caatcaccga
     2341 gccgctcacc atcggccgcg acgtggccat gatcaagctc ctctccggca aggtgcatct
     2401 catgcagccg gtatcgggcc gaacgactgg actggtgttc gagggcgaag gacgtttcac
     2461 gatggccgtg cccgacacga tcgagcgcgc gcagttgcgc cgcttctccc ggcatgacat
     2521 ctcctcggtc gacgtccgct tcacgcagct cgtcctgcga gtctcggacg acacgatcga
     2581 caagctcttt cccggaccgc aggctcagtg ggcaaagaat gccttggccg aaaatcgcga
     2641 aaaccactgg ctgatcgacc tcggccgcga catcgacgcc aaagtcatcg ccgcgatgct
     2701 gaatccggga gcgctgcaga tgacggcggc gatcaaaacg cccgactacg actggctcac
     2761 attcgactac gactcgaccc gccgcgaaga gatccagttg atccgcttcg accgccaaac
     2821 cgaagaggtg tggatcagcc tcgatcgagc caaggatcgt gatccgagtg gcggtcacgg
     2881 cgagtccgac gtccggccgg ccaccattag tagcctcgac atcaaagccg atctctcgaa
     2941 aggcaaatgg aactcgcgcc gggtcggcga gaccgatcaa gccatgctca acggtcacta
     3001 cacgatcgcc gaagtgatca cgccgaacat cgacggcatc ggcgcgctgg cgatggagct
     3061 ctacccgacc gcgaagaatg tcaccgcgat cgacgacaag gggcagccgc tgacgatcct
     3121 tcgagacaac attggcgcac gcagctcggg catcagcaac gacgtgtccg acagcaccat
     3181 gaccgtgatc ttccccgcgc cgctgcgagc aaacgcttcc cgcgtcgtca cgttccaata
     3241 cgacctcgaa acgccaaact acgcacccgg aaacgcctgg tatccgacga ttcccgatgc
     3301 cttcgacgcc acgaccgcac atatcgagtt gaaggtcagc aagcgcaatg aagtgcgctc
     3361 gatgggtaag cgaatcagcg agacggaagc ggcggatgga acgaagacat cggtttggct
     3421 cgtcgagaag cccgcgaaga tggtcacgtt ctcgaccgcg gagcgcttcg tcgaagttcc
     3481 actcgagctt cccggagctc cgaaggtcat ctcgttcggc acggtcactg gtctggatcc
     3541 gggcaaccgg atccgcaacg ccggcgcgga cgtcatcaac tcgcttcgct tctatcaatt
     3601 gctcttcgac gacaagctcg acacgcccca gatctatgca accgccatcg ccgcggggca
     3661 cggacaggcg ttcgacggct tcctccactt atccgagttc tcctacgagg agcatcccgg
     3721 cgcaacggag cttttccgca cgcatgaagt ggctcacgag tggtggggtc acaaggtcgg
     3781 ctggaaaacg taccgcgatc agtggctcag cgagtcgttc gccgaatact cggcgatgat
     3841 gttcgtgcag gcgacggtga agggcggcga caagtactac gacgagatcg tcagcgtgta
     3901 cgacagcatc gtgcagggca acatgggcgg cggattcagc aagttcaacc ggccttggct
     3961 ggtcgagttc aatcccagtg cgcgcgctcg catcggaccg atcggcgtcg gctatcgcgc
     4021 tgctacagcc gaggtgccgg cggggtacga gatccaggcg tatttcaaag ggccgctcgt
     4081 actgcacatg cttcgaacaa tgttgcggtt caagaccggt cacgacgatg ttttcttcaa
     4141 aatcctgcgc gacttcatca aggactacag cggcaaagac gccagcaccg ccgacttcca
     4201 gaaagaggtc gagaagaatg cgccaggcga ctggagcttc ttcttcaatt cctggatcta
     4261 cgactccgcc attcccacaa tccgctggag ttacaaggtc gaaccgacgg caaccggcgg
     4321 atcaaagatc agcgtcaccg tgaagcgcag cggcgtgggc gatgactttg tgatggtggc
     4381 gccggtgcgc ctcgaattcg acggtaaccg cgccggcatg ttctacgtac tgatcaacaa
     4441 gaacgagcag accgtgaccc atgagctctc gtccacccct cggaatgtca tcttcgcgcc
     4501 ggatcattcg ttgctcgcga acatccggcg ggagtaatct gttgccactg ctcgcccagg
     4561 agagaccctc aaacatgcgc cttacctcga tcgtggcaat ggccatcatc gcagcctcac
     4621 cgctggccgc cgccgtgcgc agcgcccgcg cgccgcacga gatgcggcag acgacgccac
     4681 cagcgattcc gaagagcggc gtccgcgctg agttgcttca cgatctcgac gacatgcaga
     4741 agaagattct ctcgctggct gcggcggttc cggcttcgaa gtacgactgg cgtccggcgc
     4801 acggcgtccg ttcggtgagc gaggtctaca tgcacatggc cggcggcaac tacacgctgg
     4861 ttagtttcgt cggcgaccat ccgatgctcg atacacgttc gttcgagcgg atcacggaga
     4921 agccgcgcat catcgaggag ctccgcaaat cgttcgacta cctgcgcgac gcgatcatca
     4981 acacctcgga cgccgatctc gacaaatcga ttcacatgtt cggcaacgac agcaccgagc
     5041 gcgccgcctt catgctggcc ctcaaccacc tccacgagca cctcggccaa tccgtcgcct
     5101 acgcgcggat gaacggcatc gtgccgccgt ggagcacgga gtagggagat cgtctcacca
     5161 cagagacact gagatcacag aggcgtcgct ttccactcta tgatctctgt gtctctgtgg
     5221 ttcatgattc ttcgcccgtc tacgcctcca ccagttcctt cgcgaacatc cctggcgtgt
     5281 aggcggtgac gtggcccgtg aacgctgacg cggcgacggt cagaggcgag gcaagccaca
     5341 acttgccggg gccgctgcgg cctttgtagt tgcggttgat cgcgctgatc gtgacttgat
     5401 cggagtcctc ggacactcca ggaccgcaac caatgcaggc gccacagccg ggattgagga
     5461 cgatggcgcc ggcgcgctcg aacgtatcga gcagaccgtt ctcacgtgcg aacgtctcga
     5521 cagcctgcga gccgaactgg atgtagaact tcacgccctc cgccactttc aatccggcat
     5581 ccacagcctc cttcgctacc tggtggtaga agaggaagtc gtccaccttg cccgcggtac
     5641 aggagccggc gtaagcgatg tcgatcttca catcgctgac ttcgtcgacg aacgctccgt
     5701 tcgtcggatc ggatgggacg ccgtgatcgg gatcgcccgg atgcgccacc atcggacgga
     5761 tctcgctgag gtcgatcgtg tgcacgccgc ccgcgtacac cgcgtcggga tccggagcga
     5821 ccgcacgcgc acggagcgct tcgatatcgg cctcgggacg catggcggcg atccaggcga
     5881 atgtcttttc gtccgcctcg atgatcgccg ttcgcgccgc gcactcggtg gccatgttcg
     5941 ccagcgtggc gcgctcgtcc atcgacaacg agcgcagacc gggaccggtg aactccatga
     6001 tccgatccag cgtcatctgc ggcttggcgt agttcaggag gatgtacagc atcacgtcct
     6061 tcgccgtgac gttctcacgc agagtcccca cgagctcgaa gcggatcgac tccgaaacct
     6121 caacctgcgt aaaccccgaa tgcacgaggt tcgcgtactc cgtggcaccg acgccccagg
     6181 ccaacgcgtt gttcacgccg cccatgcag
//
LOCUS       DATLVW010000203        12369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_255649, whole genome shotgun sequence.
ACCESSION   DATLVW010000203 DATLVW010000000
VERSION     DATLVW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255649"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..2290
                     /gene="speA"
                     /locus_tag="VNN25_06625"
     CDS             350..2290
                     /gene="speA"
                     /locus_tag="VNN25_06625"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HXH91237.1"
                     /translation="MEAQPEQFDERAPWTVDDASSLYMIDRWGTGYFDVNPAGDLTVA
                     PLQESGIAVPIIDVLREAQVLNLNTPLLIRFQDLLRHRVETLNNAFNSAITEHQYRGS
                     YRGVFPIKVNQLREVVEEILDAGRPFNYGLEVGSKPEMFAGLAIHSDPEALIVCNGYK
                     DTTFIRMALLGRKLGKKVILIAEKLSEVRAITRIAAEMGVEPWIGMRVRLLSKGKGKW
                     ATSGGEHAKFGLSTAEILEAIDILREAKMESAFKLLHFHIGSQIPDILTIKRAVREAA
                     MYYAKLHKLGHPLEYLDVGGGLAIDYDGSRSEFHSSMNYTLEEYASDIVYNIMDVCDD
                     EKVPHPNIVSESGRAIVAHHSVLVVQAFGAIEKTPLEPRTVQATDHKLTREILDIDQS
                     LSDENVNEAWHDLQQIKEQSQQMFELGLLPLDVKARIETLYWEIAEKLQSIIRHMDPE
                     EVPEDLRTLNIELADQHICNFSVFQSLLDHWALGQLFPIVPIHRLNERPQLESTLVDI
                     TCDSDGKVSKYIDRNDVRETLPLHELRDGAPYYLGIFLTGAYQDIMGDIHNLFGRVNE
                     VHVFLDADEESGFYIEEEIPGQTIGEVLAMTQYDWRDLEKRMKTQIDAAIKQDIVRPN
                     EGMRLLQEFERGLKDQTYLTIE"
     gene            2563..3492
                     /locus_tag="VNN25_06630"
     CDS             2563..3492
                     /locus_tag="VNN25_06630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH91238.1"
                     /translation="MRKTFALCALALIVVAGNAFALGEARVQGKVTDAATHKPIENVV
                     ITLDAVTGHSFHAQYKTDKDGVYRFIVLDGTLFYKVTISAPGYSPLEQQQVKFSLGDV
                     NTRDVVLAAGGTAASGTKDAPPAKVDPGVAAYNQGAELANAGKLPEAIAKLEEAVAAK
                     PDLTAGYEALARLYLRTKNYDKAIDRANKALEVDNDNQDLFSVLAESYTAKGDKAKAA
                     EYRKKLPADATAMFNDAAKLINSNKDAEAEPLLKGAIAANDKFGQAYYELGMIYVRTQ
                     RNAEAKVNLQKYLELEPNGKDAATAKEMLNYVK"
     gene            3569..4021
                     /locus_tag="VNN25_06635"
     CDS             3569..4021
                     /locus_tag="VNN25_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91239.1"
                     /translation="MRNLLMMTALLSFAAGCASSDTTSSIEPEITLVQLSRVAEGMRS
                     DTGPVSVHYGVEVRNPTDAPIHLDRVSVQSIGGGAYDLPAHSQGFDVTIAPKESKSVD
                     FWAPAYVVTPAASGANGPVTIRATVELDAAGKKFQKISMQNVSPAGGN"
     gene            complement(4490..6355)
                     /locus_tag="VNN25_06640"
     CDS             complement(4490..6355)
                     /locus_tag="VNN25_06640"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HXH91240.1"
                     /translation="MRDFIQAIADEHVYLFDGAMGTMLYAKGVFINKCYDELNLRNPE
                     IVLEVHKQYVRAGAEILETNTYGANRIKLRSFGIEDDLREINEGGAAIARQAAGDSVF
                     VAGAIGPLGIRIEPYGPTSRDEAREYFREQAAALRDAGVDLFVLETMSNIAEIEQGIL
                     AIRDICSLPIVAQMTIGNDGRTIYGDVPRTIAQRLDAAGADVIGLNCSVGPDVMLDAI
                     EEMTAVTAKKISAQPNAGLPREVNGRQMYMASPDYMAKYAKRLIQKGVKFLGGCCGTT
                     PEHIKVMADAVRPLSPRRSFVVIEHDADGKNSESKTAVPFAERSNWGKKIAAGEFVTT
                     IEITPPKGPNPDKMIESVHAIREAGVDAVNVPDGPRAQNRMGAIAVAVLLQQRVGIET
                     VLHYCCRDRNLLGMHSDMLGCAALGLRNLLLITGDPPKMGPYPDATAVFDIDSIGLTN
                     MANLMNHGLDLGGNPFGEPTKFTIGVGVNPGHLDLDYELRRLDWKVKAGAEYAITQPV
                     FDAAILEKFLEQIEDMHLPVIAGIWPMLSYRNAQFMNNEVPGVSVTDEVMERMRIASE
                     KSKEHGLREGVTIARETLERVRHRVAGVQVSAPLGRVDLALQVFEGLVETKAISS"
     gene            complement(6542..9532)
                     /locus_tag="VNN25_06645"
     CDS             complement(6542..9532)
                     /locus_tag="VNN25_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91241.1"
                     /translation="MRCLSLLLLLLLVATSAFGASPSTVNNDDSCDVMVAPAATLLLP
                     YFEVDFTTNRVQDTLFTITNVTRLPQIAHVTIWTDWSYPVLAFNIYLTGYDVQGISLN
                     QVLGGTVAQPAGTGPTVFQTQPSGAGSLSAGFTANPNFAGGGASINCANQPGALSPTL
                     VAALRSALSTGVYNPGGSAACPDAVGGVHANAIGYVTIDVVSNCSLQLPTDPLYYSSS
                     LLFDNVLIGDYQQLGPTASGTTAAAFDAGVNPLVHIRAVPEGGSAGSKVATALPYTFY
                     DRYTPALTRTIDRRQPLPSTFAARFIQGGPATYRTNLNIWREGIGNGSCSDAKSSATM
                     AVAEIVRFDEHENPSTLGIDPSRSPRLPVTSSTSSSDSSYPAMLSSDTGGWLYLNLNN
                     GGSTTYSAHAGASTNEASSLAPAGSTTTAGPRPSQNWVTVTMFGTGSQGNRQAAAFDA
                     ASLGTGCSPAAFPSYAAAIGPAGGVFDCPPGTTLTNGSIALCAQTTATPATPAASAER
                     RRATGRSAPSTSIDNDDSCDIKVGPAATLLLPYFEVDLAGTSGQTTLFTVTNVTQYPQ
                     IAHVTLWTDYAYPVMTFNLFLGGYDVQAINLADVLIRNTVAPPNGTSGPNPGSIWPAT
                     NPNLRTSLDCAGIPGVIPGTIMAEVRRALTTGGTSSCSRPVGSVHANAIGYATVDLVA
                     SCTSRSPSDPLYYTNDLLFDNVLIGDYQQLVPHPTGVTPTSSDATGNPMVHIRSVPEG
                     GGAGSSVSTRLTYTFYDRYTPAGARTMDRRQPLPSAFAARYIQGGPRAFATNLTIWRE
                     GFGIGSCADALASGAMIVSEIVRFDEHENFTVIDDCIGIGECATSPRTLNAASSTNTA
                     SSIYSYISTADVGGWFYLNLNNGGSTSYSVTKDIGSQRAPTNARSNLAPIGSSTTKGP
                     RPSQNWMTVTLFGFANSGNRLTGEFDAAPLGNGCSPAAFITTANFGGASIGPAGGVLV
                     CPPGTTPADGSTTKCTGTNINPLP"
     gene            9699..11180
                     /locus_tag="VNN25_06650"
     CDS             9699..11180
                     /locus_tag="VNN25_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91242.1"
                     /translation="MIVPVLLFLTSAAASPVATATATRDAIASPHATMRELGLVPRAP
                     IPHGEEHEQRRSFASKIRAVAAEAPIRSESVVTKPSLAAPRVLSSFAGSHDLGWNPSD
                     AAGAVSSKYLLHASNASILVQDRTGAILSNIPLESFWHDPAYTDGTVYDSRVLYDAVA
                     DRWILCTLYDVNYNKSTLLIAVSDNGNPSLGWHRYRFLVDPNDIREADATRAALTRDS
                     IVITANIYTDNSESSDLYIVRKTDAYAGTATLPITDVQTQGIELTPVDGRDDAIVYLV
                     TPSNTEIDIYSVSGSTFTSVGSPQPPPLGIISGQAGGSAPQLGSTLKLDCGYISVPNA
                     VLRNGTLWVASQIDMNNPFRTSVMWWRIGMSTPLKVDTGIVDDPTGATMYAFPSIAVN
                     KLGAALIGYSVFSASIYPSGGYSYIDPFNSLSKPAVMKAGDGPSHLSRWSDFSGTVVD
                     ANDVDFWTIQTYAPTFIAANGYWATWWARIEMPALARRRAALH"
     gene            complement(11264..>12369)
                     /locus_tag="VNN25_06655"
     CDS             complement(11264..>12369)
                     /locus_tag="VNN25_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXH91243.1"
                     /translation="VRALVGGSDYNLSPFDRTSAMHRQPGSAFKTFAYLAAIASKKAT
                     TASLLLDAPVSIEVSGNETWQPHNYDGRYRGRVTLREAFEHSLNVPTVRLSEDIGVSR
                     VVDTAEKFGFEEKFAKIPALPLGVTEVSMRELTAAYTPFPNLGVRVEPYLLREVRNSA
                     GKQLYVHELEQKRVANADATYVLHTLLRGVVQRGTASRLKRYGLGYVAGKTGTTSDYR
                     DAWFVGYVPDMVTSVWVGFDHGAPLRLSSGESAIPIWGAYMSSIPHLHSEPRAPAGVT
                     FREIDSETGMLWQDGCPPPIQEVFLDGTAPTHKCPAGFFGNIVRRIFFDRGNFDEPPA
                     ITLEEFRKWANDVDRSRRDVEGAWDKLRRIFGR"
BASE COUNT         2439 a         3766 c         3896 g         2268 t
ORIGIN      
        1 aagatcaaga acggatccgt cgagctggga cgtggcgtaa aagaaggcct cggccaggtc
       61 gcgcagaaag cgggcgaagg actgcagaag gccggcaaga aggccgagga cgacgtcaag
      121 aaaacggaga cggcggcgaa gcactgatcg ctcccaaacg cgaggttgtg gggtcaactt
      181 gaaagacctg tcaccacaga ggcacagagg tcacagaggt ccctctgtgt tctctgcgtc
      241 tctgtggttg cctttgcctc ggcgaccacg ataagcgtcc aaattgacct acgaccgaaa
      301 gtagaacagt tcggtgaagt aggtccgcga tggtctatcc ttccgcgcga tggaagcaca
      361 acccgaacag ttcgacgagc gggccccgtg gacggtggac gacgcatcgt cgttgtacat
      421 gatcgaccgc tgggggaccg gctattttga tgtgaacccg gccggcgatc tcaccgtcgc
      481 gccgctgcag gaaagcggca tcgcggtgcc gatcatcgat gtcttacgcg aagcccaggt
      541 gctgaacctg aacactccgc tgctgatccg ttttcaggac ctgctgcggc accgcgtcga
      601 gacgctcaac aacgccttca acagcgcgat caccgagcat caataccgcg gcagctatcg
      661 cggcgtcttc ccgatcaagg tcaaccagtt gcgcgaggtc gtcgaagaga ttctcgacgc
      721 cggccggccg ttcaactacg gcctcgaagt cggctccaag ccggagatgt tcgcgggcct
      781 ggccattcac agcgatcccg aggcgctgat cgtgtgcaac gggtacaaag acaccacctt
      841 catccgcatg gcactccttg gccgcaagct cggcaagaaa gtgattctca tcgctgagaa
      901 gctttcggaa gtgcgtgcca tcacacgcat cgccgcggag atgggcgtcg aaccgtggat
      961 cgggatgcgc gtccgtctgc tctccaaagg caaaggcaag tgggctacct cgggtggcga
     1021 gcacgcgaag ttcgggttgt ctactgccga gattctcgaa gccatcgaca tcctccgcga
     1081 ggcgaagatg gagtcggcgt tcaagctgct ccacttccac atcggctcgc agattccgga
     1141 catcctcacg atcaagcgcg ccgtccgtga agcggccatg tactacgcca agctgcacaa
     1201 gctcggacat cctctcgagt acctcgacgt cggcggcggg ctggctatcg actacgacgg
     1261 ctcgcggtcg gagttccact cgtcgatgaa ctacacgctc gaggagtatg cgagcgacat
     1321 cgtctacaac atcatggacg tctgcgacga cgagaaagtg ccgcatccga acatcgtttc
     1381 ggagtccggc cgggccatcg tcgcgcatca ctccgtgctc gtcgtgcagg cgttcggcgc
     1441 catcgagaag acgccgctgg agccgcgcac ggtgcaggcg accgaccaca agctgacgcg
     1501 cgagatcctc gacatcgatc agagcctgag cgacgagaac gtgaacgagg cctggcacga
     1561 cctgcaacag atcaaggagc agtcgcagca gatgttcgag ctcggcctcc tgccgctcga
     1621 cgtcaaggcg cgcatcgaga cgctttactg ggagatcgcc gagaaactgc agagcatcat
     1681 ccggcacatg gatcccgaag aagtgccgga ggatctgcgc acgctcaaca tcgagctggc
     1741 cgatcagcac atctgcaact tctccgtctt ccagtcgctg ctcgatcact gggcgctggg
     1801 acagctcttt ccgatcgttc cgattcatcg cctcaacgaa cggccgcagc tcgaatcgac
     1861 gctcgtcgac atcacctgcg actcggatgg caaggtgtcg aagtacatcg accgcaacga
     1921 cgtccgcgag acgcttcccc tccacgagtt acgcgacggc gccccctact acctcggcat
     1981 ctttctcacc ggcgcgtacc aggacatcat gggcgacatc cacaacctct tcggccgcgt
     2041 caacgaagtg catgtcttcc tcgacgccga tgaagagagc ggcttctaca tcgaggaaga
     2101 gatccctggc cagacgatcg gggaagtgct tgccatgacc cagtacgact ggcgcgatct
     2161 ggagaagcgg atgaagacgc agatcgacgc cgccatcaag caggacatcg tccggccgaa
     2221 cgaaggaatg cgcctgctcc aggagttcga gcggggactc aaggatcaga cctatctcac
     2281 gatcgagtaa cggacgtgtg attcaacgca cggttgagcg tgcatcgttc ggtcagcggt
     2341 tgcctacccg cctgtgtcat ccttcgcgcg ttgagtcaac acgggtcaaa ggaaaacccg
     2401 gtcaccacag aggcacagag gtcacagaga aacctctgtg ttctctgtgt ctctgtggtt
     2461 ggctttgctt cggcgaccac gataagcccc gccccaaacc ggaatcggtg actggatcgg
     2521 ggcgttaact tgcgacgaac attcaaccga caggagaagc aaatgcgtaa gacattcgcg
     2581 ctgtgtgcgc tggccctcat cgtcgtggcc ggtaacgcgt tcgcgctcgg cgaagcgcgc
     2641 gttcagggga aggtcaccga tgcggcgact cataaaccga tcgagaatgt ggtcatcact
     2701 ctcgacgcgg tcaccggaca cagctttcac gctcagtaca agaccgacaa agacggcgtc
     2761 taccgtttca tcgtgctcga cggtacgctc ttttacaaag tgaccatttc cgctcccggt
     2821 tactcccccc tcgagcagca gcaggtcaag ttctccctcg gcgacgtcaa cactcgcgac
     2881 gtggtactcg ctgctggcgg aactgccgcg agcgggacaa aggatgcgcc cccagccaag
     2941 gtcgatcctg gcgttgccgc gtacaaccag ggcgcggagc tggctaacgc gggcaaactg
     3001 cccgaggcaa tcgccaagct cgaagaggcc gtcgcggcaa agccggatct gaccgccggc
     3061 tacgaagccc tggccaggct ctatctgcgt accaagaact acgacaaggc gatcgaccgc
     3121 gccaacaagg ctctcgaagt cgacaacgac aaccaggatc tcttctcggt gctggccgag
     3181 tcgtacaccg ccaagggcga caaggcgaaa gccgccgagt accgcaagaa gctgccggcc
     3241 gacgccacgg cgatgttcaa cgacgcggcc aagctcatca acagcaacaa ggatgcggag
     3301 gccgagccgc tgctcaaagg cgcgatcgcc gcgaatgaca agttcgggca ggcctactac
     3361 gagctcggca tgatctacgt gaggacgcag aggaacgccg aagccaaggt caacctgcag
     3421 aagtacctgg aactggagcc gaacggcaag gacgccgcta cggccaaaga gatgctgaat
     3481 tacgtgaagt gaaacaaccc gacagctcga ttgttaaatg atgaccgggg gggggcggaa
     3541 acgctccccg cattttttgg agaccatcat gcgaaatcta ctgatgatga ccgcgctcct
     3601 gtcgttcgca gccggctgtg caagcagcga cacgacgtct tcgatcgaac cggaaatcac
     3661 gctcgtgcag ctctcccggg ttgccgaggg gatgaggagc gataccggtc cggtgtcggt
     3721 gcactacggg gtcgaagtga gaaaccccac ggatgcgccc attcaccttg accgcgtgag
     3781 cgtgcaatca atcggcggcg gagcctacga cctgcctgca cactcgcagg gcttcgatgt
     3841 gacgatcgct ccgaaagaga gcaagtcggt cgacttctgg gcgcctgcgt acgtcgtcac
     3901 tccggctgca tccggtgcga acggcccggt gacgatccgg gcgacggtgg agcttgatgc
     3961 ggccgggaag aagttccaga agatcagcat gcagaacgtc agcccggcgg gcgggaactg
     4021 agatcgtcgc cccgtcgcta ccagttcgac caaccgcgcc ttgcaaaaga gggacaggga
     4081 gaggcggtcc gggtcgaact ctatccgaat gtgagtgcca tgcaatcaac gccgagtctc
     4141 atgcgaaaga ctccgagtct cttgcatatg actccgagtc tcatgcgaaa gactccgagt
     4201 ctcatgcgaa agactctgag tcttatgcga aagactccaa gtctcatgcg aaagactctg
     4261 agtcttatgc gaaagactcc cgagtctaat gcgaaagact ctgagtctct tgcgaaagac
     4321 tcccgagtct catgcgaaag accctgagtc tcatgcgaaa gactcccgag tctcttgcat
     4381 acgactccga gtctctcgcg aaagactctg agtcaggcga caggctggcc cggtgacttt
     4441 ccacgtggaa cgcgaccggc tgacgcagag ccgatcgcgg gcagccttgt tacgagctga
     4501 tcgccttcgt ctccaccagc ccctcgaata cctgcaacgc cagatcgaca cggccgaggg
     4561 gcgctgagac ctgcacaccg gccacgcggt ggcgcacgcg ctcgagcgtc tcgcgcgcga
     4621 tggtgacgcc ttcgcgcagg ccgtgctcct tgctcttctc gctggcgatg cgcatccgct
     4681 ccatcacttc atcggtcacg gaaacgcccg ggacttcgtt gttcatgaac tgggcgttgc
     4741 ggtacgagag catcggccag attccggcga tgaccggtag gtgcatgtcc tcgatttgct
     4801 cgaggaactt ctccagaatc gcggcgtcga agaccggctg ggtgatggcg tactcggcgc
     4861 cggctttcac tttccagtcg aggcggcgca gctcgtaatc gaggtcgaga tggccgggat
     4921 tgacgccaac gccgatcgtg aacttcgtcg gctcaccgaa cggattcccg ccgaggtcga
     4981 ggccgtggtt catcaggttg gccatgttcg tgaggccgat cgaatcgatg tcgaacacgg
     5041 cggtggcatc gggatacggc cccatcttgg gaggatcgcc ggtgatgagc agcagattgc
     5101 gcaggccgag cgccgcgcat ccgagcatgt cggaatgcat gccgaggagg ttgcggtcgc
     5161 ggcagcaata atgaagaacc gtttcgatgc cgacgcgctg ctggagcagg acggctacgg
     5221 cgatcgcgcc catgcggttc tgcgcgcgcg gcccgtcggg aacgttgacg gcatcgacgc
     5281 ccgcctcccg gatcgcatga acggactcga tcatcttgtc ggggttcggt cccttgggcg
     5341 gcgtgatctc gatcgtcgtg acgaactcgc ccgcggcgat cttctttccc cagttggagc
     5401 gctcggcgaa cggcacggcg gtcttcgact cgctgttctt gccgtcggca tcgtgctcga
     5461 tgacgacgaa cgaccggcgc ggcgacagcg gccggaccgc atcggccatg accttgatgt
     5521 gctccggcgt cgtgccgcag cagccgccga gaaacttcac gcccttttga atgagccgct
     5581 tcgcgtactt ggccatgtag tcgggcgagg ccatgtacat ctgccgcccg tttacttcgc
     5641 gcggcagacc ggcgttcggc tgcgcggaga tcttcttcgc cgtgaccgcc gtcatctcct
     5701 cgatggcgtc gagcatgacg tccggcccga ccgagcagtt gagtccgatg acgtcggctc
     5761 cggcggcatc gaggcgctgg gcgatggttc gcggcacgtc gccgtagatc gtccggccgt
     5821 cgttgccgat cgtcatctgc gccacgatcg gaagcgagca gatgtcgcgg atcgcaagga
     5881 tgccctgctc gatctcggcg atgttcgaca tggtctcgag cacaaagagg tcgacgccag
     5941 cgtcgcgcaa cgcggcggcc tgctcgcgga agtactcgcg ggcttcgtcg cgtgaggtcg
     6001 gtccgtaagg ttcgatgcgg atgccgagcg ggccgatggc gcccgcgacg aagacggaat
     6061 cgccggcggc ctggcgtgcg atggcggcgc cgccctcgtt gatctcgcgg aggtcgtcct
     6121 caatgccgaa gctgcgcagt ttgatgcggt tcgcgccgta ggtgttcgtc tccaggatct
     6181 cggcgccggc gcggacgtat tgcttgtgaa cttcgagcac gatctccgga ttgcggagat
     6241 tgagctcgtc gtagcacttg ttgatgaaca cacccttggc gtagagcatc gtgcccatgg
     6301 cgccgtcgaa gagatagacg tgctcgtctg cgattgcctg gatgaagtcg cgcataaaaa
     6361 aagccccacg ccggattcga cgtggggcca aaatcgaaga acgaattcga acttgccggc
     6421 tatttagctt tttttaccct ggttgcaagc gaccccaaat caatccagcg agttgcgaat
     6481 gtaggccgtg cgcggttatt tgtcaaacgc gggtaaccag acgccgatcg tcgccggcgg
     6541 ctcacgggag cgggttgatg ttcgtgccgg tacatttcgt cgtcgagccg tcggcgggcg
     6601 tcgtgcccgg cgggcagacc aggacgccgc cggcggggcc gatagaagcg cctccgaaat
     6661 tcgcagtggt gatgaacgct gccggcgaac agccgttgcc gaggggggca gcgtcgaact
     6721 cgccggtgag gcggtttccg gagttggcga aaccgaacaa cgtgacggtc atccagttct
     6781 ggctggggcg agggcccttc gtggtcgaag aaccgatggg ggccaggttc gacctcgcat
     6841 tcgtcggagc tctctggctc ccgatgtctt ttgttaccga gtagctcgtg ctgccgccgt
     6901 tgttgaggtt gaggtagaac caaccaccga cgtcggcggt tgaaatgtac gaataaatgc
     6961 ttgaagctgt gttggttgaa gacgcggcgt ttagcgtgcg gggcgatgtt gcgcattccc
     7021 ctatcccgat gcaatcgtca atgacggtga agttttcgtg ctcatcgaat cggacgatct
     7081 ccgaaacgat catggctccg gacgcgagtg catccgcaca actgccgatg ccgaagccct
     7141 ctcgccagat ggtgaggttc gtcgcgaacg ccctcggacc accctgaatg tagcgcgctg
     7201 caaacgctga aggaagcggt tgccggcggt ccatggtccg tgctcccgcc ggcgtgtaac
     7261 ggtcgtaaaa cgtgtacgtc agccgggtac tgacgctgga acccgcgccg ccgccctcag
     7321 gtacggaccg aatgtgcacc atcggattgc ctgttgcatc gctcgaggta ggagtcacgc
     7381 cggtagggtg cggtacgagt tgctggtaat cgccgatcag cacgttgtcg aagaggaggt
     7441 cgttcgtgta gtagagcgga tccgacggag agcgggacgt gcaactcgcc accaggtcga
     7501 cggtcgcata gccgatcgcg ttcgcgtgga cgctgccaac agggcggctg catgagcttg
     7561 tcccgccggt tgtgagcgct ctgcgcacct ccgccatgat ggtccccgga atgactcccg
     7621 gaatgcccgc gcaatcgagc gaggtgcgca gattggggtt ggtggccggc cagatcgatc
     7681 cgggattcgg gccggacgtg ccgttcggcg gcgcgaccgt gtttcgtatg aggacatcgg
     7741 cgaggttgat ggcctgcacg tcataaccgc ccagaaacag gttgaaggtc atcaccggat
     7801 aggcgtagtc ggtccagagc gtgacgtgcg caatctgcgg gtattgcgtg acgttggtga
     7861 ccgtgaagag agtcgtctgt ccggaggtgc cggctagatc gacctcgaag tacggcagca
     7921 gcagcgtcgc ggccgggccg accttgatgt cgcaactatc gtcgttgtcg atcgacgtgg
     7981 aaggcgccga ccttcccgtg gctcgccgtc gctccgcaga agcagcagga gttgccggag
     8041 tcgcggtcgt ctgtgcgcac aacgcgatgg agccgttcgt gagcgtcgtt ccgggcgggc
     8101 agtcgaacac tccgccagcg ggaccgatcg ctgccgcgta gctcgggaat gcagcaggcg
     8161 agcagccggt gccgagcgac gctgcgtcga acgccgccgc ctgtcggttg ccctggctgc
     8221 cagtgccgaa catcgtcacg gttacccagt tctggcttgg acgcgggccg gcggtggtcg
     8281 ttgagccagc cggggcaagg ctggacgctt catttgtcga agcgccagcg tgggccgagt
     8341 aggtcgtact tccgccgttg ttcagattga ggtagagcca gccgccggtg tcggagctga
     8401 gcatggccgg ataggaagaa tccgacgatg acgtcgagga cgtgactggt aaccgcggcg
     8461 agcgtgaggg gtcaattccc agcgtggacg gattctcatg ctcgtcgaag cggacgatct
     8521 ccgctacggc catcgtcgct gacgacttgg catcgctgca gctcccgttg ccgattccct
     8581 cgcgccagat gttgaggttt gtgcggtatg tggccggccc gccctgaatg aaccgcgccg
     8641 cgaacgtgga cggtaacggc tggcgacggt cgatggtccg ggtcaacgcc ggcgtgtacc
     8701 ggtcgtagaa ggtatacggc agcgcggttg cgaccttcga gccggcgctg ccgccttcgg
     8761 gcaccgctcg aatgtgaacc agaggattga ctcctgcatc aaacgctgcc gccgtcgttc
     8821 cggacgcagt gggtccaagc tgctggtagt cgccgatgag aacgttgtcg aacagaagcg
     8881 acgacgagta gtagagcgga tccgttggca gttgcaacga gcagttcgac acgacgtcga
     8941 tcgtgacgta gccgatcgcg ttcgcgtgaa ccccgccgac ggcgtccggg catgcggcgg
     9001 aaccacccgg attgtagaca ccagtgctaa gagcgcttct gagcgcggcg acgagcgtgg
     9061 gagacagcgc tcccggctgg ttggcgcagt tgatcgaggc gccaccaccc gcgaagttcg
     9121 ggttggcggt gaagccggca gacagcgagc cagcacccga cggctgcgtc tggaacacag
     9181 tcgggccggt gccggcgggc tgagcgaccg tgccgcccag aacctggttc aacgagatac
     9241 cctgcacgtc ataacccgtg aggtagatgt tgaaggcgag gaccgggtac gaccagtccg
     9301 tccaaatggt gacgtgagcg atctgcggca agcgcgtcac gttcgtgatc gtgaaaagcg
     9361 tgtcctgcac ccgattggtg gtgaaatcca cttcgaagta cggcaggagc agcgtggccg
     9421 cgggagcgac catcacgtca caggagtcat cgttgttgac cgtcgaagga gaggcgccaa
     9481 acgcggacgt tgcgacaagg aggagcagga gtagaagcga taggcatctc acagtttttc
     9541 aaggctccgt acggcagttt atgccttatg acatggaggt cttcggtgac cccagacact
     9601 agtgaccatg tccgtgcaag ggacccccga atcaattcag cgagtggcga attgtggccg
     9661 tcacagactc tttgtcaaat ccccggtacc atgccccggt gatcgttcct gtcctgctct
     9721 ttctcacttc ggcggcagcc tcgcctgtgg caaccgcaac cgccacgcgc gacgcaatcg
     9781 catcaccgca tgccacgatg cgcgagcttg gcctcgtgcc gcgcgcaccg atcccgcacg
     9841 gcgaggagca cgagcagagg cgatcctttg cgtcgaagat ccgcgccgtc gccgccgagg
     9901 ctccgatccg cagcgagtca gtcgtcacca agccgtcgct tgccgcgcct cgggtcctct
     9961 ccagcttcgc cggcagccac gaccttggct ggaatccctc ggacgccgcg ggcgctgtct
    10021 cctccaagta tctccttcac gcttcgaacg cgtccatcct cgtacaggac cgcaccggtg
    10081 ccatcctcag caacatccca ctcgaaagct tctggcatga cccggcctac accgatggca
    10141 cggtttacga ctcccgtgtt ttgtacgacg cggtcgcgga ccgctggatc ctttgcacgc
    10201 tctacgacgt caactacaac aaatcgacgc tgctgatcgc ggtctcggac aacggtaatc
    10261 cgtcgctagg ctggcaccgg taccgttttc tggtcgaccc gaacgacatc cgggaagccg
    10321 acgctacgcg cgccgccctg acgcgcgact cgatcgtgat cacggccaac atctacaccg
    10381 acaacagcga atcgtccgac ctttacatcg ttcgcaagac ggacgcctac gcaggaactg
    10441 ccaccctccc gataacagat gtccaaacgc aaggcatcga actgactccg gtagacggtc
    10501 gcgacgacgc catcgtctac ctcgttacgc cgtccaacac ggagatcgat atctactcgg
    10561 tcagcggctc gacgttcacc tctgtcggta gtccgcagcc gccgccactg ggcatcatca
    10621 gcggtcaagc gggcggaagc gcgcctcagc tcgggagcac gctcaaactc gactgtggat
    10681 atatttcggt gcccaacgcg gtattgagaa acggaacgct gtgggtagca tcccagatcg
    10741 acatgaacaa tccgtttcgc accagcgtca tgtggtggcg gatcgggatg agcacgccgc
    10801 tgaaggtcga tacgggaatc gtcgatgacc caacgggtgc aacgatgtat gccttccctt
    10861 cgattgcggt gaacaagttg ggcgcagcct tgatcggtta ctcggtcttc agcgcttcca
    10921 tctatccgtc gggcggctat tcgtatatcg atccgttcaa cagcctgagc aagccggcag
    10981 tgatgaaagc gggtgacggg ccttcgcacc tttcccgctg gtcggatttt tcgggcaccg
    11041 ttgttgatgc caacgacgtc gacttctgga cgattcagac ctacgcgccg accttcatcg
    11101 ccgccaacgg ttactgggcc acgtggtggg ccagaatcga aatgccagct ctggcgcggc
    11161 ggcgtgccgc gctgcattag cgaaaaaccc ttaaccacag aggcacagag gtcacagaga
    11221 actgtctttc tctgtgatct ctgtgtctct gtggtgatcg cttctatcgc ccgaagattc
    11281 ggcgcaactt gtcccatgcg ccctcgacgt cgcgacggct tcgatcgacg tcgttcgccc
    11341 acttgcggaa ctcttccaac gtgatcgcgg gaggctcgtc gaagttgccg cgatcgaaaa
    11401 agattcgtcg gacgatgttg ccaaagaagc cggccggaca cttgtgcgtc ggcgcggttc
    11461 cgtcgagaaa aacttcctga atcggcggcg gacagccgtc ctgccagagc atgccggtct
    11521 cggagtcgat ctcgcgaaac gtcacgccgg caggcgccct cggctccgaa tggagatgcg
    11581 gaatggagct catgtacgcg ccccagatcg gaatggccga ttctcccgac gagagccgga
    11641 gcggcgcgcc gtgatcgaag ccgacccaga cgctggtgac catgtcagga acgtagccga
    11701 cgaaccaggc atcgcggtag tcgctggtcg tgcctgtttt gccggcgacg tagccgaggc
    11761 cgtagcgctt cagccgcgag gccgtgccgc gttgaacgac gccgcgtagc agggtgtgca
    11821 agacataagt cgcgtcggcg ttggccacgc gtttctgctc gagctcatga acgtagagtt
    11881 gcttcccggc actgttgcgc acttcgcgga gcaggtacgg ctcgacgcgt acgccgaggt
    11941 tcgggaaggg cgtgtacgcc gcggtcagct cgcgcatcga cacctcggtc acgccaagcg
    12001 gcagcgcagg aatcttcgcg aacttctcct cgaatccgaa cttctctgcg gtgtcgacga
    12061 cgcgcgagac gccgatgtcc tcgctcagcc ggacggtcgg aacgttcagc gaatgctcga
    12121 atgcctcgcg cagcgtcacg cggcctcgat agcggccgtc gtagttgtgc ggttgccagg
    12181 tttcgttgcc actaacctcg atcgacaccg gcgcatcgag caggagcgag gccgtcgtcg
    12241 ccttcttcga ggcaatcgca gcgaggtagg cgaatgtctt gaacgccgag ccgggctggc
    12301 gatgcatggc cgaggttcga tcgaaagggg agaggttgta gtcgctgccg ccgaccaatg
    12361 cgcgaacgc
//
LOCUS       DATLVW010000204         3465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_378953, whole genome shotgun sequence.
ACCESSION   DATLVW010000204 DATLVW010000000
VERSION     DATLVW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378953"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1939)
                     /gene="ettA"
                     /locus_tag="VNN25_06660"
     CDS             complement(260..1939)
                     /gene="ettA"
                     /locus_tag="VNN25_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HXH91244.1"
                     /translation="MATNTEPNKIIYSMIGVSKFHGKKQVLKDIYLSYFYGAKIGVIG
                     LNGSGKSSLLRILAGTDRDFLGETVISPGHTVGFLEQEPQLDPEKTVREIVEQGVQPV
                     IDLLKEFDEINNAFGDPDADMDKLMERQGKVQDKLDAAGAWDLDSKLEMAMDALRCSP
                     GDTLCGILSGGEKRRVALCRLLLQSPDILLLDEPTNHLDAESVEWLEKHLQQYPGTVI
                     AVTHDRYFLDNVAGWILELDRGAGIPFKGNYSSWLEQKQARLQHEEKSESQRQKTLER
                     ELEWIRMSPKGRHAKGKARINSYEELLSQQTQEKVQDLEIYIPPGPRLGNIVIEADHV
                     RKAFGDTLLIDEMEFKLPPGGIIGVIGPNGAGKTTLFRMILGQEPADAGTIRVGETVT
                     LGYIDQNRTLDPEKSIFEEITGGTELVLLGTREMNSRAYVARFNFSGSDQQKKVGILS
                     GGERNRVHLAKMLKEGANVLLLDEPTNDLDVNTMRALEEALETFAGCAIVISHDRWFL
                     DRVATHILAFEGDSRVVWFDGNYSEYEEDKHKRLGAEADQPHRIRYRQMTR"
     gene            complement(2201..2521)
                     /locus_tag="VNN25_06665"
     CDS             complement(2201..2521)
                     /locus_tag="VNN25_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91245.1"
                     /translation="MIRSDIGMICFDIGMICSGIGMICFDIGMICSGIGMICSDIGMI
                     CSDIGMICSGIGMICSGIGMICSDIGMICSGIGMICSDIGMIRSGIGTKHSDLLPPSP
                     SFGR"
     gene            complement(2661..>3465)
                     /locus_tag="VNN25_06670"
     CDS             complement(2661..>3465)
                     /locus_tag="VNN25_06670"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXH91246.1"
                     /translation="TYEDLAPHVKRIVERERVKAEIDAANRIQAALLPVDAPEVAGAA
                     FASHYRAATEIGGDYFDFLTMPSGEIGIAFGDVSGHGLTSGIVMAMAKSALLVQVGYD
                     PGPRAVMNVLNDIVMKTAPRRILMTFFFGLLDPDTQTLRFSSAGHLDPYVYRAGEKRL
                     EALSSWGFPLGVKRREPFTEHTVDFDAGDRLILYSDGLIEAIDDDGDPFGFDRFEKTI
                     LACGHMHAEDIKKTLLNSIRKFTRNRPPEDDQTLVVVSFEEEARVRAAS"
BASE COUNT          643 a          995 c         1088 g          739 t
ORIGIN      
        1 gccgtttaac cggcgttcac cgggaaggcg catcctacgc gctgagccgg atcgttgcaa
       61 gacttgggag cccaagattg cgcttcgcgc gttgtcggtt gtcagttgtc ggttgattcg
      121 agcgatcggt tttgttcgtc agagatcagt ggattagtct actagtggac cagtggatta
      181 gtgaatcgtc agagatcgtg accgctcacc ctgatctctg aatctctgat ctctgcctct
      241 tcctgacctc agcttccgct caccttgtca tctgccggta gcggatccgg tgcggctggt
      301 cggcttcggc cccgagccgc ttgtgcttgt cttcttcgta ctcgctgtag ttgccgtcga
      361 accagacgac gcggctgtca ccttcgaatg cgaggatgtg cgtggccacg cggtcgagaa
      421 accagcggtc gtgggagatg acgatcgcgc atcctgcgaa cgtctccagc gcctcttcca
      481 gtgcgcgcat cgtgttgacg tcgaggtcgt tcgtcggctc gtcgagcagc agcacgttgg
      541 cgccttcctt caacatcttc gcgaggtgaa cccggtttcg ctcaccgccc gagaggatgc
      601 cgactttctt ctgctgatcg gagcctgaga agttgaagcg ggccacgtag gcgcgtgaat
      661 tcatctcgcg cgttccgagg agcaccaact cggtgccgcc ggtgatttct tcgaagatcg
      721 atttctcggg gtcgagcgtg cggttctgat cgatgtatcc gagcgtcacc gtttcgccga
      781 cgcggatcgt gccggcgtcc gcgggctcct ggccgaggat catgcggaag agcgttgtct
      841 tgccggcgcc gttcgggccg atcacaccga tgatgccgcc gggcggaagt ttgaactcca
      901 tctcgtcgat gaggagcgtg tcgccgaacg ccttccgtac gtgatctgcc tcgatgacga
      961 tgttgccgag gcgcgggccg ggcgggatgt agatctcgag gtcctgcact ttttcctgcg
     1021 tctgctggct cagcagctcc tcgtacgaat tgatgcgcgc tttgcctttg gcgtgacgcc
     1081 ccttcggcga catgcggatc cattcgagct cgcgctcgag ggtcttctgc cgctgcgact
     1141 ccgatttttc ttcgtgctgc aggcgggcct gcttctgctc cagccacgac gagtagttgc
     1201 ctttgaacgg gatacctgcg ccgcggtcga gctcgaggat ccatccggcg acgttgtcga
     1261 ggaagtagcg gtcgtgtgtg acggcgatga cggtcccggg atattgctgg agatgttttt
     1321 ccagccactc caccgactcg gcgtcgagat gattggtcgg ctcatcgagc agcagaatgt
     1381 ccggcgactg gagtaacagc cggcagagtg cgacgcgccg cttttcgccg ccggagagga
     1441 tgccgcagag cgtgtcgccc ggcgagcagc ggagcgcgtc catcgccatc tccagcttgg
     1501 agtcgaggtc ccaggcgccc gcggcgtcga gcttgtcctg gaccttgccc tggcgctcca
     1561 tcaacttgtc catgtcggca tccggatcgc cgaaggcgtt gttgatctcg tcgaactcct
     1621 tgagcaggtc gatgaccggc tgcacgccct gctcaacgat ctcgcgaacg gttttctcag
     1681 gatcgagctg cggctcctgt tcgaggaagc cgacggtgtg gcctggggag atcaccgtct
     1741 cgccgaggaa gtcgcggtcg gtgccggcga ggatgcgcag cagagacgat ttgcctgaac
     1801 cgttcaggcc gatgacgccg atcttcgcgc cgtagaaata ggagaggtag atgtccttga
     1861 ggacctgctt cttgccgtga aacttcgaga ctccgatcat cgagtagatg atcttgtttg
     1921 gttcggtatt ggtagccatg gggcgcggat tgtaatggag tgcggtcgtc tcgaccgcgg
     1981 gcactcgggc gtcccgcccg agtgctgtca gggcggacgc cggaatttgt cggcgtgctt
     2041 gcttccctga cgtacagctt cgacatctcg ccgccgctga aaacgctggc ggtgcggtct
     2101 gaaacggttc agacgaaggt gagacgggcc agccggaaaa aggtcatgca accgggaaga
     2161 agagcgccaa aagacgggaa tgggcggtaa tgagccggaa tcagcggcca aaagacggag
     2221 agggcggtaa taagtcggaa tgttttgttc cgatgccgga acgaatcatt ccgatgtcgg
     2281 aacaaatcat tccgatgccg gaacagatca ttccgatgtc ggaacaaatc attccgatgc
     2341 cggaacagat cattccgatg ccggaacaaa tcattccgat gtcggaacaa atcattccga
     2401 tgtcggaaca aatcattccg atgccggaac aaatcattcc gatgtcgaaa caaatcattc
     2461 cgatgccgga acaaatcatt ccgatgtcga aacaaatcat tccgatgtcg gaacgaatca
     2521 ttccgatgcc ggaacgaatc gtttcgatgc cggaatggtc gattccgaaa tcgcaatggt
     2581 cggttcgatc gtgcgacgaa ggcgctcgac tggagaaagg gtcagtttgt cacgagtgcg
     2641 aaagtgtggg ccagctcagg ctaacttgcc gcccgcactc ttgcttcttc ctcgaacgag
     2701 acgaccacca gcgtctggtc gtcctcggga ggccggttgc gcgtgaactt tctgatcgag
     2761 ttgagaagcg ttttcttgat gtcctctgcg tgcatgtggc cgcaggcgag gatcgtcttc
     2821 tcgaagcggt cgaagccgaa cggatcgcca tcgtcgtcga tcgcctcgat caatccgtcg
     2881 ctgtagagga tgagccggtc gccggcgtcg aagtcgactg tgtgctcggt gaacggctcg
     2941 cggcgtttga cgccgagcgg gaagccccac gacgaaagcg cttcgagacg tttctcgccg
     3001 gcgcggtaga catagggatc gagatgacct gccgacgaga agcgcagcgt ctgcgtatcc
     3061 ggatcgagca gaccgaagaa gaacgtcatg aggatgcgcc gcggcgcggt cttcatgacg
     3121 atgtcgttca acacattcat caccgcgcgc ggccccgggt cgtagccgac ctgaaccagc
     3181 agcgccgact tcgccatcgc catcacgata ccgctggtca ggccgtgacc agagacgtcg
     3241 ccgaatgcga tgccgatctc gcccgaaggc atcgtcagga agtcgaagta atcgccgccg
     3301 atctcggtgg cggcgcgata gtgcgatgcg aacgccgctc ccgcaacctc gggagcatcg
     3361 accggcagga gtgcagcctg aatgcggttc gcggcatcga tctccgcttt cacgcgctcg
     3421 cgctcgacga tccgtttgac gtgcggcgcg aggtcttcat acgtg
//
LOCUS       DATLVW010000205         4347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_544129, whole genome shotgun sequence.
ACCESSION   DATLVW010000205 DATLVW010000000
VERSION     DATLVW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544129"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VNN25_06675"
     CDS             <1..607
                     /locus_tag="VNN25_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HXH91247.1"
                     /translation="PLGSGDDFARVLGIPRDLRAACELALRGSAREVDVAVANGIRYL
                     GVAGLGFDSEVARYANESVKFLRGSLVYLYAILRVLPRFTPHRVTIDGNRNEEIMFAT
                     VGNSRQYGGGIRITPDAKIDDRELDLCIVHRTSRMQLLKTLPKAYSGAHVKSPFVETG
                     RGTEFSFGSERPMDVYADGERITMTPVRFGLAEERLKIVAP"
     gene            916..1503
                     /gene="clpP"
                     /locus_tag="VNN25_06680"
     CDS             916..1503
                     /gene="clpP"
                     /locus_tag="VNN25_06680"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXH91248.1"
                     /translation="MVLIPMVVEQTSRGERAYDIYSRLLKDNVIFLGSQIDDTVSNLI
                     IAQLLFLEAENPEKDISIYINSPGGSITAGLAIYDTIQYVKPDIQTICIGQAASMAAV
                     LLAAGKKGKRFALPNSRVLIHQPLMHGLGGQASEIDIHAKDIMRMKARMSQILADHTG
                     QPFEKVDRDTDRDYILQAQEAVDYGLVDQVISKRE"
     gene            1615..2865
                     /gene="clpX"
                     /locus_tag="VNN25_06685"
     CDS             1615..2865
                     /gene="clpX"
                     /locus_tag="VNN25_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXH91249.1"
                     /translation="MSKKGNSGDVLRCSFCNKSQRDVKKLIAGPTVYICDECVDICLD
                     IIAEDRKDEPGTMEGSGQLPKPKEIKEFLDLYVIGQERAKKKLAVAVYNHYKRIDYHT
                     RNNRQDVELQKSNILLIGPTGTGKTLLAQTLARMLSVPFTIVDATTLTEAGYVGEDVE
                     NIILKLYQAAGNDKDKTQRGIVYIDEVDKICRKGDNPSITRDVSGEGVQQALLKILEG
                     TVANVPPQGGRKHPHQEFIQIDTTNILFICGGAFVGLENVIERRLSATSMGFGAKIAT
                     KKEKRTGELLAQVHPEDLIKFGLIPEFVGRLPVVATLSELDKGTLIEILREPKNSLVR
                     QYQTIMGFENVILKFHDDALEAIAEEALKRNVGARGLKIIIEELMLEIMYTIPSQDAI
                     SECVITREVVINRSQPITLYRKAG"
     gene            2958..>4347
                     /locus_tag="VNN25_06690"
     CDS             2958..>4347
                     /locus_tag="VNN25_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXH91250.1"
                     /translation="MPNKPEITTLPLVPLRDMVVFPSMMAPFVVGRRASILALEATLA
                     TAGKKIFLATQKDPKIDDPSIGEIYPIGVVATVVQNLKLPNQNVKVMVEGLTRAEIIS
                     TQEADGYVTVTVRDIESAVPLTAEVAQYMQKLVEIFESYAKMSHHLAFEGVMSTLKLD
                     DPDKFADTLASHILITTPEKQQLLETISPYERLQKLQDFLEVEIEKINIDKRINSKVK
                     KQMERAQKEYYLNEKIKAIHHELGRKDDRSDEVEELRQKIEEAGMPKETKEKALQELK
                     RLEAMPGVSAEATVSRNYIEWLVSVPWKKTTKEIKDIDLAESILNEDHYGLDKIKERI
                     LEFLAVRQLIKGPKGTILCFVGPPGVGKTSLAKSIARATGRKFVRLSLGGVRDEAEIR
                     GHRRTYIGAFPGQIIQRMKKAGTINPVFLLDEVDKMSMDFRGDPSAALLEVLDPEQNN
                     AFTDHYLDVEYDL"
BASE COUNT          982 a         1371 c         1255 g          739 t
ORIGIN      
        1 cccgctcggg tccggcgatg atttcgcgcg tgttctcggc atccctcgcg acctgcgcgc
       61 tgcgtgcgag cttgcgttgc gcggcagtgc gcgggaggtc gatgtcgccg tcgccaacgg
      121 tatccgctac ctcggcgttg ccggactcgg cttcgattcg gaagtggccc gctatgcgaa
      181 cgagagcgtc aagttccttc gcggctcgct cgtctatctc tacgccatcc ttcgcgttct
      241 gccgcgattc accccgcatc gcgtgaccat cgacggtaat cgcaacgaag agatcatgtt
      301 cgccaccgtc ggcaactcgc ggcaatacgg cggcggcatc cgcatcacgc ccgacgcgaa
      361 gatcgacgat cgcgagctcg acctctgtat cgttcatcgc acctcgcgga tgcaactgct
      421 gaaaacgctg ccgaaagcct actcgggagc gcacgtgaag agcccgtttg tcgagacagg
      481 gcgtggcacc gagttcagct tcggctccga gcgcccgatg gatgtctacg ccgacggcga
      541 acggatcacg atgacgccgg tacggttcgg gctggcagaa gagcggctga agattgtcgc
      601 gccgtgaaca aggagtgcga gcgtctcgct cgcggatatc ggggcgtcct cgccccgata
      661 ttttgtccgg cgagccctcg gtatccgctg catcagtcag attctgcggg ggcttgtggc
      721 tagccttcat tccgcgacaa ccctctcgca cccgggcgag gacgcccgag tgcccgcggt
      781 cgagacgacc gcacttcttg cgggcataga cccacacaaa gccctcaaaa gcactaccca
      841 tacggtcagg agaccctgcg gaattcggcg ctagactcgc tgcattcaac gggtcgccca
      901 aggagacacc aacgaatggt cctgatcccc atggtggtcg agcagacgag tcgtggcgag
      961 cgtgcctacg acatctactc ccgcctcctc aaagacaacg tcatttttct cggatcgcag
     1021 atcgacgaca cggtctcgaa cctgatcatc gcccagctcc tctttctcga agcggagaac
     1081 ccggaaaagg acatctcgat ctacatcaac tcgccgggcg ggtcgatcac ggccgggctg
     1141 gcgatctacg acacgatcca gtacgtgaag cccgacatcc agacgatctg catcgggcag
     1201 gcggcgtcga tggctgcggt gctgcttgcg gccggcaaga aggggaagcg gttcgcgctc
     1261 ccgaactcgc gcgtcctcat ccaccagcca ttgatgcacg gcctcggcgg acaggcctcg
     1321 gagatcgaca tccacgccaa ggacatcatg cgcatgaaag cgcgcatgag ccagatcctt
     1381 gccgatcaca ccgggcagcc gttcgagaaa gtcgatcgcg ataccgatcg cgactacatc
     1441 ctccaggcgc aggaagcagt ggactacgga ctcgtcgatc aggtgatctc gaaacgcgag
     1501 tgatcgcgtg aaatagcgtg ttgttatgga gtgcacggaa tggtctcgcg aaggcttcac
     1561 gttcacgaaa gcgcggcgta gaatctaacc acctcaaaag gatccaagcc agcaatgtcg
     1621 aagaagggaa acagcggcga cgtcctccgt tgcagtttct gcaacaagag ccagcgcgac
     1681 gtcaagaaac tgatcgccgg cccgacggtt tacatctgcg acgaatgcgt cgacatctgc
     1741 ctcgacatca ttgctgagga tcgcaaagat gagccgggga cgatggaagg ctccggccag
     1801 ttgccgaagc cgaaagagat caaagagttc ctcgatctct acgtcatcgg ccaggagcgg
     1861 gccaagaaga agctcgccgt cgcggtctac aaccactaca agcggatcga ctaccacacg
     1921 cggaacaacc gccaggatgt cgagctgcag aagtcgaaca tcctgctgat cggaccgacc
     1981 ggcaccggca agacgcttct ggcgcagacg ctggcccgca tgctcagcgt accgttcacg
     2041 atcgtcgacg ccacgacgct gaccgaggct gggtatgtcg gcgaggacgt cgagaacatc
     2101 atcctcaagc tctaccaggc cgccggcaac gacaaggaca aaacgcagcg cggcatcgtc
     2161 tacatcgacg aggtcgacaa gatctgccgc aagggcgaca acccatcgat cacccgcgac
     2221 gtcagcggcg aaggcgtgca gcaggccctg ctcaagatcc tcgaagggac cgtcgccaac
     2281 gttccgccgc agggtggacg caagcatccg catcaggaat tcatccagat cgatacgacg
     2341 aacatcctct tcatctgcgg cggagcgttc gtcgggctcg aaaatgtgat cgagcggcgg
     2401 ttgtcagcga cctcgatggg cttcggcgcc aagatcgcca cgaagaaaga gaagcgcacc
     2461 ggcgagctgc tggcgcaggt tcatcccgag gatttgatca agttcggtct gatcccggag
     2521 ttcgtcgggc ggctgccggt cgtggccacc ctcagcgagc tcgataaggg aacgctcatc
     2581 gagattctgc gcgagccgaa gaactcgctc gtgcggcagt accagaccat catgggcttc
     2641 gagaacgtca tcctgaagtt ccacgacgac gccctcgaag caatcgccga agaagcgctc
     2701 aagcgcaacg tcggcgcccg cggcctcaag atcatcatcg aagagctgat gctcgagatc
     2761 atgtacacga tcccgtcgca agacgcgatc agcgaatgtg tcatcacacg cgaggtcgtc
     2821 atcaaccgca gccagccgat cacgttgtac cggaaggctg ggtagtacca aagttgccgg
     2881 gtgatccatg ttgcccggtt cccggaatcg gtaactcggc atctccgaag cccaacagga
     2941 aaaaactaac taacccaatg cccaacaaac cagaaatcac gactctccca ctcgtcccgc
     3001 tgcgggacat ggtcgtcttc ccctcgatga tggcgccgtt cgtcgtcggc cgccgtgcgt
     3061 cgattctcgc gctcgaggcg actctcgcca cagccggaaa gaagattttc ctggcgacac
     3121 agaaagatcc gaagatcgac gatccttcga tcggcgagat ctatccgatc ggcgtcgtcg
     3181 ccaccgttgt ccagaacctc aagcttccga accagaacgt gaaagtgatg gtcgagggtc
     3241 tcacacgcgc cgagatcatc tccacgcagg aagcggacgg ctacgtcacc gtgaccgtcc
     3301 gcgacatcga aagcgccgtg ccgctgaccg ccgaggtcgc gcagtacatg cagaagctgg
     3361 tcgagatctt cgagtcgtac gcgaagatgt cgcatcacct ggcgttcgaa ggcgtcatgt
     3421 cgacgctgaa gctcgacgat cccgacaagt tcgccgatac cctggcctcg catatcctca
     3481 tcacgacgcc cgagaagcag caactcctcg aaacgatctc gccgtacgag cgcctgcaga
     3541 agctgcagga cttcctcgaa gtcgagatcg agaagatcaa catcgacaag cgcatcaact
     3601 ccaaggtcaa gaagcagatg gagcgggcgc agaaggagta ctacctcaac gagaagatca
     3661 aggccatcca ccacgagctt ggccgcaaag acgatcgctc cgacgaggtc gaagagctcc
     3721 gccagaagat cgaagaagcg ggcatgccga aagagacgaa ggaaaaagct ctgcaggagc
     3781 tcaagcgtct cgaagcgatg cccggcgtgt ccgcggaagc gaccgtctca cgcaactaca
     3841 tcgagtggct cgtctccgtt ccctggaaga agacgacgaa agagatcaag gacatcgatc
     3901 tcgccgagtc gatcctcaac gaagatcact acggcctcga caagatcaaa gagcgcatcc
     3961 tcgagttcct ggccgtgcgc cagctcatca aaggaccgaa ggggacgatc ctctgcttcg
     4021 tcggacctcc gggcgtcggc aagacgtcgt tggcgaagtc gatcgcccgc gctaccggac
     4081 gcaaattcgt ccgcctttcc ctcggcggtg ttcgcgatga ggccgagatt cgtgggcatc
     4141 ggcgaaccta catcggcgcg ttccccggcc agatcatcca gcggatgaaa aaggcaggca
     4201 cgatcaatcc cgtcttcctc ctcgacgaag tcgacaagat gtcgatggac ttccgcggcg
     4261 atccctcggc cgcccttctc gaagtcctcg atcccgagca gaacaacgcc ttcaccgatc
     4321 actatctcga cgtcgagtac gacttgt
//
LOCUS       DATLVW010000206         7344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_343240, whole genome shotgun sequence.
ACCESSION   DATLVW010000206 DATLVW010000000
VERSION     DATLVW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343240"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VNN25_06695"
     CDS             <1..255
                     /locus_tag="VNN25_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXH91251.1"
                     /translation="IRVEDRGAGIAARDLPHLFEPFYRGRGADRVRGSGLGLTIVKQI
                     AAAHGGSVTAERRREHGAAFTLRLPARSVAEPVSEIAQHA"
     gene            248..943
                     /locus_tag="VNN25_06700"
     CDS             248..943
                     /locus_tag="VNN25_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH91252.1"
                     /translation="MPELQRRILLVEDEESLILTLRDRFESEGYAVTVQSDGESALAA
                     GTRSPFDLIVLDVALPKKNGFDVCRDLRQRGIQTPILMLTARGQVVDRVVGLKLGADD
                     YLTKPFEMMELLARVEALLRRSRTMAANASDSFAFGPIHVDFRSAEVRREGVSVELST
                     LEIKLLRYFVEHRGAVVGRDELLDKVWGYDATPVTRTVDVHVASLRQKLEANPSKPEY
                     IVTVHGLGYKFTG"
     gene            1060..2055
                     /locus_tag="VNN25_06705"
     CDS             1060..2055
                     /locus_tag="VNN25_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXH91253.1"
                     /translation="MPLVDQDISDPACASDEVIVEIRAAGICHSDAHYRAGRGHVALP
                     LTLGHEIAGVVLEIGDDVTNVAVGDRVAVHYLVSCGTCPDCVRGGEQFCADGEMFGKE
                     RDGGFAEWVVIPARNAVLIPQAIPFDQAAVMMCSTATVYHALRLAGLQPGESLAIIGF
                     GGLGVSALQLAGAFGASSITAIDVVPEKLAAAAALGAVGIDARRDDLANEIRRITGGH
                     GADVVVDLAGIAAPRLAALQSLAPGGRLVLVALGPEPFSFDPYSDVLTRERRIIGCSD
                     HLLTELPELMDLARRGDIDLSSAITRRVPLEAKAINEVLDDLDRGTSHLRALVTR"
     gene            complement(2169..2591)
                     /gene="tsaE"
                     /locus_tag="VNN25_06710"
     CDS             complement(2169..2591)
                     /gene="tsaE"
                     /locus_tag="VNN25_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXH91254.1"
                     /translation="MTQFLCRTESETEAAGCAIARTLAPDAVVHLIGDLGAGKTFLAR
                     AIAAELGANPLDVASPSFAIVHEYPVAGRAPIIHIDGYRLTDNRREWLEIGIPELLQG
                     PGVKLIEWPKGAFTEFEDGHVEIAIRVLDDGTREINMT"
     gene            2668..2877
                     /locus_tag="VNN25_06715"
     CDS             2668..2877
                     /locus_tag="VNN25_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH91255.1"
                     /translation="MAQTKVSSKYQIVIPKELRAEIDIRPGQKFQMVAKGGVITLVPD
                     KPISEMRGFVRGIRTSGFREKKDRF"
     gene            2874..3251
                     /locus_tag="VNN25_06720"
     CDS             2874..3251
                     /locus_tag="VNN25_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH91256.1"
                     /translation="MIVLDSSGWLEFFADGPYADEFAKRLRQPGNVLTPTIAIYEVYK
                     WLKRERSEQEAVQAVATMQKTAVVEVTKEVAVSAADLSLEHKLAMADSIMLAVAQAYD
                     AELLTTDSDFEGIAGVSVFSKKP"
     gene            3311..5755
                     /locus_tag="VNN25_06725"
     CDS             3311..5755
                     /locus_tag="VNN25_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH91257.1"
                     /translation="MAIHSADNQADAAYGMSETVAAALLMFQRYLNEEIPPTAASYAL
                     ATLMAQPPDVLMQQVATWTAERSQAGSGAPRDLLLLALKKVYITGELNLLDREAVANY
                     LDRVTGVALRLCPDADRDQLRSDLSTMRMSRSTTASLQEMVAPARMPTINVPVPVSES
                     EAQAAKQYSLIFDRLTRERAEAGPKGAAADPQAIAQLLSMAASRSQNAQQLNQYLEQL
                     RPLAGGGEGNVFVILGGAMPSWDVANLLPGTTARAPSQVGAMEKIIDLAESPEVAMKR
                     FRELVTAAVEKFNQGALGAALWMLDVAEDSIPEKKLEIAAVDQFRAEAVEMISAMQLR
                     KYAETKSKHAAVKIALEFFPTLHLETLFRNLRGEKRAEQRRAFLGFIEAYGVIGRNAA
                     LDDLERELQRPDVDTYYLRNLIYLLHRIQRESQDSIGREFDLLEKSSEPGLIIYVIKE
                     AVTALGLIKTDAAVKVLTTRLAQFEATLLRNDTSTHPAAEMQKLLDRITGALARIGTS
                     AALLTIARHGMKANPPLGDTRARLASLAQYDLSFDESTVNILLKALREEIPGKLLGRL
                     LPRVQDGTVRLMEALSGTRTDETEDIFRDIARRFPDQDVGRAAAQALAKWSPEEAVTR
                     PDAAATLAGELEFFGLPSVMQSLGEMRATGMLTLTNKQKLPVAKLVFVDGKFLNAQTG
                     HIRGDEALYQTLERPVPGAFAFVPYPPEKLVSDIVPREIMGLLLEGMRRNDELQRLVA
                     LVPDDMILTKTSFKPTPHPEEEDPILAREVWLKASSGARIAECERQLATDSFRVRRIV
                     AHWLEEGALVESAPAS"
     gene            complement(6049..>7344)
                     /locus_tag="VNN25_06730"
     CDS             complement(6049..>7344)
                     /locus_tag="VNN25_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXH91258.1"
                     /translation="RSVVPMVFIVGDRTKIGGDAAANLAICERLGLQIHDISHDDSLN
                     EALVHASDADLVIDAIFGTGLNRAPEGLYAETIVSMMELGLPIVAVDLPSGANASSAE
                     TFEPCVQAALTITFAAPKICHVFEPAAVSCGEVIVADISIPTAAIEDENITLALTTPS
                     DVRPHFAARLAATHKGTYGHVAIIAGSPGRSGAAVLAARGAIRSGAGLVTVATDAETA
                     KLVNAGSIESMTYVGDDVLAFAKSKDAVLIGPGLPDDKNSYARVRGLVAAIERPLVVD
                     ASALNAFESRASDINPSRHPRVLTPHPGELARLLGTTASEVNAHRIDVAREAARLTGC
                     VVVLKGFQSLIAEPDGHVNVNPTGNPGMAAGGMGDVLGGMIAALLARGIDPFHAAIAA
                     VYVHGFTGDMLKEEMGDTGLTAGDLAERIPLAIKRLRET"
BASE COUNT         1332 a         2298 c         2369 g         1345 t
ORIGIN      
        1 attcgtgtcg aagaccgggg agcagggatt gccgcgcgcg atctgccgca tctgttcgag
       61 ccgttctatc gcggacgcgg ggccgatcgc gttcgtggca gcggcctcgg cctcaccatc
      121 gtcaagcaga tcgccgccgc gcacggaggg agcgtcaccg ccgagcgccg cagagagcat
      181 ggcgcggcgt tcacgctccg gctgccggcg cgctccgtcg ccgagccggt ttcggagatt
      241 gcgcagcatg cctgagctgc agcggcgcat tctgctggtc gaagacgaag aaagtctgat
      301 cctgacactg cgcgaccgct tcgagtcgga gggatacgcg gtcaccgtgc agagcgacgg
      361 cgagtcggcg ctggccgcgg gaacgcgctc gccattcgac ctcatcgtgc tcgacgtggc
      421 actgccgaag aagaacggct tcgacgtctg ccgcgacctt cgccaacgcg gaatccagac
      481 gccgatcctg atgctgaccg cgcgcggcca ggtcgtcgac cgcgtcgtcg gactcaaact
      541 cggagcggac gattacctga ccaagccctt cgagatgatg gagcttctcg cccgcgtcga
      601 agcgctgctg cgacgctccc gaacgatggc cgcgaacgcg tcagactcat tcgctttcgg
      661 tccgatccac gtcgacttcc gcagcgccga ggtgaggagg gagggggtgt cggtagagtt
      721 gtcgacgctg gagatcaaac tgctccgcta cttcgtcgag caccgcggcg ccgtagtcgg
      781 gcgggatgag ttgctcgaca aagtgtgggg ctacgacgcc accccggtca cgcgaacggt
      841 cgacgtacac gtggcgtcgc tgcgacagaa actcgaagcg aatccgtcga agccggagta
      901 catcgtgact gtgcacgggc tcgggtacaa gttcactggg tgatcacgcg aaggcgcgaa
      961 gacgcgaagg gatttgcgag ggtttcttcg cgtcttcgcg tcttcgcgtg accggtgtat
     1021 gttccgcgca tgcaatccgt ccgcttgatt gaacccggca tgcctctcgt ggaccaggac
     1081 atctccgatc ccgcatgcgc ctccgacgaa gtcatcgtcg agatccgcgc cgccggcatt
     1141 tgccatagcg acgcgcatta ccgcgccggg cgtggccacg tcgcgctccc gctgaccctc
     1201 gggcatgaga tcgccggcgt cgtcctcgag atcggcgacg acgtcaccaa cgtcgcggtt
     1261 ggcgatcgag tggccgtgca ttacctcgtc tcgtgcggaa catgccccga ttgcgttcgt
     1321 ggcggcgagc agttctgtgc agacggcgag atgttcggca aggagcgcga cggcggcttc
     1381 gccgagtggg tcgtcatccc cgctcgcaac gcggtgctca ttccgcaagc cattccgttc
     1441 gatcaggctg cggtaatgat gtgctcgacc gccaccgtct accacgcgct gcggctggcc
     1501 ggactgcaac ccggcgagtc gctcgccatc atcggattcg gcggccttgg cgtctccgcg
     1561 ctgcaacttg ccggcgcgtt cggagccagc tcgatcaccg caatcgacgt cgttcctgag
     1621 aaacttgccg ccgcagcagc cctcggcgca gtcggcatcg acgcccggcg cgacgatctg
     1681 gcgaacgaga tcaggcgcat caccggcggt catggagccg acgtcgtcgt cgatctcgcc
     1741 ggcatcgcag ccccgcgcct tgccgccctg caatcgctcg cccccggcgg ccgcctcgtc
     1801 ctcgtcgccc tcggccccga gcctttcagc ttcgacccat attccgatgt gctcacccgc
     1861 gaacgccgta tcatcggctg ctccgaccac ctcctgaccg agctccccga gctaatggac
     1921 ctcgcccgcc gcggcgatat cgacctgtca tcagccatca ctcgccgcgt cccgcttgag
     1981 gcgaaggcga tcaacgaagt gctcgacgat ctcgaccgcg gcacaagcca cctgcgtgcg
     2041 ctcgtcacgc gatgaaacca ctcacgcgaa gacgctcacg cgaaggcgcg aagccgcgaa
     2101 gaggttgatc gtttttcttt cgcgccttcg cgtcttcgcg tcttcgcgtg agcggcttcg
     2161 cgtaccaatc acgtcatgtt gatctcacgc gtcccgtcat cgagcacccg aatcgcgatc
     2221 tccacatgcc catcctcgaa ctccgtgaac gcacccttcg gccattcgat cagcttcact
     2281 cccgggcctt gcagcagttc ggggattccg atctcaagcc attcgcggcg gttatcggtg
     2341 aggcggtagc cgtcgatgtg gatgatcggg gcgcggcccg cgaccggata ttcgtgaacg
     2401 atcgcgaacg atggcgacgc cacgtccagc ggattcgctc ccagctccgc ggcgatggcg
     2461 cgggcgagga aggtcttgcc tgcgccgagg tcgccgatca ggtggacgac ggcatcgggc
     2521 gcgagcgtgc gtgcgatggc acagccggcg gcttcggttt cggattcggt gcggcagagg
     2581 aactgagtca cgagcactga ttatcagcgt tttgtcatgt ccgatcgtat tacgataata
     2641 tcgatcgatg agttaaggag gagattaatg gcgcaaacga aagtttcatc gaagtaccaa
     2701 attgtgattc cgaaggagct tcgcgcagag attgacatcc gaccgggcca gaagtttcag
     2761 atggtggcca agggtggggt gatcacgctc gttccagaca aaccgatctc ggagatgcgc
     2821 ggatttgtcc gcggcattcg gacgagcggc ttccgggaga aaaaggatcg gttttgatcg
     2881 tccttgattc ctccggatgg ctcgagttct ttgccgacgg tccatacgct gacgagtttg
     2941 caaagcgatt gcggcagccc gggaacgtgc tgacgcccac gattgcaatc tatgaggtct
     3001 acaagtggct caagcgcgag aggtcggagc aggaagcggt tcaagccgtg gcgacgatgc
     3061 agaagaccgc tgttgtcgaa gtcacaaaag aggttgcggt gtccgcagcg gatctcagtc
     3121 tcgaacacaa gcttgcgatg gccgattcga tcatgctggc cgtggctcag gcatatgacg
     3181 ccgagctcct gacgactgac tccgactttg aagggattgc tggcgtcagc gttttttcga
     3241 agaagccgtg atcgctcccc ggatttcttg atcggcagag ccatcactcc ggtgcgcaag
     3301 gtcgcttagg ttggcgatac actccgccga caaccaggcg gatgcggcgt acggaatgag
     3361 cgagacggtt gcggcggcac tcttgatgtt tcagcggtat ctcaacgagg agatcccgcc
     3421 gactgctgcg tcgtatgcgc tggcgacgtt gatggcgcag ccgcccgacg tcctcatgca
     3481 gcaggtggcc acgtggacgg cggagcggag ccaggccggc tcaggtgcgc ctcgcgatct
     3541 tcttctcctc gcgctgaaaa aggtctacat cacgggcgag ctgaaccttc tcgatcggga
     3601 agcggtggcg aattacctcg accgcgtgac cggagtcgcg cttcggcttt gcccggacgc
     3661 cgaccgcgat cagttgcgga gcgacctctc gaccatgcgc atgtcgcgtt caacgactgc
     3721 gtcgctgcag gagatggtcg cgcccgcgcg aatgccgacc atcaacgttc ccgtgccagt
     3781 cagcgaaagc gaggcgcagg cagccaagca gtattcgctg atttttgatc gcctgacacg
     3841 ggagagggcc gaagccgggc cgaagggggc ggcagccgat ccgcaggcga tcgcgcaact
     3901 cctttcgatg gcagcttcgc gatcgcagaa cgcgcagcaa ctgaaccagt atctcgagca
     3961 gctccgtcct ctcgctggtg gtggcgaggg gaacgtcttc gtgatcctcg gcggagcgat
     4021 gccgtcctgg gatgtcgcga acctgctgcc cggcaccacg gcaagagcgc cgtcgcaagt
     4081 cggagcgatg gaaaagatca tcgacctcgc tgagagccca gaggtcgcca tgaagcgatt
     4141 tcgcgagctc gtgactgcgg cggtcgaaaa attcaaccag ggcgcgctgg gagcggcgtt
     4201 gtggatgctc gatgtggccg aggactccat tcccgagaag aagctggaaa tcgcggctgt
     4261 cgaccagttt cgcgccgagg ccgtcgagat gatcagtgcc atgcagttgc gaaaatatgc
     4321 cgagacgaaa tcgaagcatg cggccgtgaa gatcgcgctc gaattctttc cgacgctgca
     4381 tctggagacg ctttttcgca accttcgcgg tgaaaagcgt gcagagcagc ggcgcgcctt
     4441 tctcggattc atcgaagcgt atggcgtaat tgggcgcaac gctgcgctcg acgatctcga
     4501 acgcgagctg caacgcccgg acgtcgacac gtactacctc cgcaacctca tctacctcct
     4561 gcatcgcatc cagcgcgagt cgcaggattc gatcggacgc gagtttgacc ttctggaaaa
     4621 gtcgtcggag ccgggactga tcatctatgt gatcaaggaa gctgtcaccg ccctcggctt
     4681 gatcaagacc gatgccgcgg tgaaggtact gacaacgcgg ctggctcaat tcgaagccac
     4741 tctgctccgc aacgacacct cgacccaccc ggccgccgag atgcagaagc tgctcgaccg
     4801 gatcaccgga gcgctggcgc ggatcggcac atcggctgcg ttgctgacca tcgcccgcca
     4861 tgggatgaag gccaatccgc cgctgggcga tacgcgcgcc cggctcgcat cgctagcaca
     4921 gtacgacctc tccttcgacg agagcacggt caatatcctg ctcaaagctc tgcgcgagga
     4981 aatccccggc aagcttctgg ggcgtctgtt gccgagggtg caggatggaa cggttcgcct
     5041 catggaggcg ctctccggaa cccgaacgga tgagaccgag gatatttttc gcgacatcgc
     5101 acgccggttt cccgatcagg acgtgggacg cgcggcggcg caagcgcttg cgaaatggtc
     5161 acccgaggag gctgtgacac gccccgacgc ggcggcgacg cttgcgggag agctcgagtt
     5221 cttcggcttg ccgtccgtga tgcagtccct cggcgagatg cgcgccaccg gcatgctcac
     5281 actcaccaac aaacagaaac tgcccgtggc caagctcgtc ttcgtggacg gaaagtttct
     5341 caatgcgcag acggggcaca tacgcggtga cgaggctctc taccagacgt tggagcgtcc
     5401 agtacccggt gcgttcgcgt tcgttccgta tcctccggag aagttggtga gcgatatcgt
     5461 gccccgggag atcatggggc tgcttctgga ggggatgcgc cgcaacgatg agctgcagcg
     5521 cctcgtcgca ctcgtgccgg acgacatgat cctgacgaag acgagcttca agccgacacc
     5581 acatcccgag gaagaagatc cgatcctggc ccgggaagtg tggctcaaag catcatcggg
     5641 cgcgcgcatc gcagagtgcg agcgacaact ggcgaccgat tcattccgcg tacgacgcat
     5701 cgtcgcgcat tggctggagg aaggcgccct ggtcgagagt gcacccgcga gctagccctg
     5761 agctgatccg cattttcaac acacattctt ggaccctcgc gccaaacccc aatcgagcgc
     5821 cttcgccccc cgcgcttttc agcgggggga gaaggtgccg aaggcggatg aggggggact
     5881 tgcggtgtcg ctgcttggtc tttgagcact gatcagccag tcgcatttgt gggattccgc
     5941 ggatgtcgtc aaacggcccc ggccttcggc caccttctcc ccatctgaga agcgatgggg
     6001 agaaggggct cgattggagc ttggagcgag atcaatgcga gctgacgctc acgtctcccg
     6061 cagacgcttg atcgcgagcg ggatccgttc cgcgagatcg ccggcggtca gaccggtgtc
     6121 gcccatttct tccttaagca tgtcgcccgt gaatccgtgg acgtagacgg cggcgatcgc
     6181 ggcgtggaac ggatcgatgc cgcgcgccag gagcgcggcg atcatgccgc cgaggacgtc
     6241 gcccatgccg ccggcggcca tgcctggatt gccggtggga ttgacgttca catgaccgtc
     6301 gggctcggcg atcagcgact ggaagccttt gagcacgacg acgcacccgg tcagacgtgc
     6361 tgcttcgcgc gcgacgtcga tgcgatgagc gttcacctcg ctcgccgttg tgccgaggag
     6421 gcgggccagc tcgccgggat gcggcgtgag gacgcgcggg tggcgcgagg ggttgatgtc
     6481 gcttgcgcgc gattcaaacg cgttcaacgc cgacgcgtcg acgacgagcg gacgttcgat
     6541 cgcggccacc agaccgcgga cgcgcgcgta gctgtttttg tcatcgggaa gcccgggtcc
     6601 gatgagcacg gcgtctttgc tcttcgcgaa cgcgagcacg tcatcgccca cataggtcat
     6661 cgactcgatc gatcccgcgt tgacgagctt cgcggtttcg gcgtcggtcg cgacggttac
     6721 cagtcccgcg ccgcttcgaa tcgctcctcg ggccgccaga actgccgctc cgctccggcc
     6781 gggcgatccg gcgatgatgg ccacgtgacc gtacgtgccc ttatgcgttg cggccaggcg
     6841 cgccgcgaaa tgggggcgca catccgacgg cgtcgtcaga gccagcgtga tgttttcatc
     6901 ctcaatggcg gcagtgggga tggagatgtc ggcgacgatc acctcgccgc aactgacagc
     6961 tgccggctcg aagacgtgac agatcttcgg cgcggcgaag gtgatcgtca gcgcggcctg
     7021 gacgcacggt tcgaaggttt ccgcgctcga tgcgttcgcg ccgctgggaa gatcgacggc
     7081 gacaatcggc agtccgagct ccatcatcga cacgatcgtc tccgcgtaca gcccctcggg
     7141 tgcccgattc agaccggtgc cgaagatcgc gtcgatgacg aggtcggcgt cggaggcgtg
     7201 caccagcgct tcgttgagcg agtcgtcgtg ggagatgtcg tggatttgca ggccgaggcg
     7261 ttcgcaaatg gcgagatttg ccgctgcgtc gccgccgatt ttcgttcgat cgccaacgat
     7321 gaacaccatc ggcacgacgc tgcg
//
LOCUS       DATLVW010000207         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_419410, whole genome shotgun sequence.
ACCESSION   DATLVW010000207 DATLVW010000000
VERSION     DATLVW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419410"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VNN25_06735"
     CDS             complement(<1..1062)
                     /locus_tag="VNN25_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXH91259.1"
                     /translation="MRKLTAPFMASLVALFVFIAPLRADDPKPADKPADKAADKAGDK
                     AGEKPPEPAKFVRQHRMKLGGADVAYTTTAEEIFLKDADGKNTASFFTIAYTKDGVAK
                     QEDRPLTFVYNGGPGSASIWLHFGLVGPKIIDIPSDAADPGSPPYKLRDNEDTIFRAT
                     DLVFIDPIGTGYSHAMGDKKGEDFWGYDEDADSVAEFIRSFITQHNRWNSPKYLLGES
                     YGGIRSALLVPRLQGNLGIGLNGVILLSPALNMGTLPFVVNGNDLTYATSLPALAATA
                     YYHHKLPDQWKDLDTLLREVEKFAGTEYLVALFQGDELPKAEKERIAERVHRYTGVSK
                     EYILRADLRIYALRFTKELL"
     gene            1152..2294
                     /locus_tag="VNN25_06740"
     CDS             1152..2294
                     /locus_tag="VNN25_06740"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXH91260.1"
                     /translation="MTVTPEPHRGRIPELDGIRGLAILLVLVWHYLVCEVGAPPPLFR
                     FLNQTWSGVNLFFVLSGFLIGGILLDHREAPNYYRVFYIRRVCRILPIYVVWLLLFYI
                     LRGPVSRLPLGTASGWLFADPLPFWSYMTFTQNFLQGKYGVWGPNWLAVTWSLAVEEQ
                     FYLLLPVIVRLMPPRRLPYLLAPFTLLAPVLRFLFFDPRMAVDPSYVLLICKADALLL
                     GVFCAWLARREGAAERIRAKAPLLRILLLSCGGFVCLAAYVPETATIRALNDSLLAIG
                     YASLILQALFTPRSPFARMARVRWLRALGLIAYGTYLIHQTVNGLAFGAVRGHWPYIT
                     QASDLLVILGALTITIAAAMASWRWFEKPIVDFGRRWRYADQASSD"
     gene            complement(2296..>3365)
                     /locus_tag="VNN25_06745"
     CDS             complement(2296..>3365)
                     /locus_tag="VNN25_06745"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXH91261.1"
                     /translation="GSVPNVGLMAQKAEEYGSHPTTFEIPFEGVVRVVDANGNVLMRH
                     KVEAGDVWRMSRAKDIPIQDWVKLAVRRARTTSAPAVFWLDSNRAHDANVITKVVQSL
                     GTMDTEGLEIRIMAPVQAMRYSLERIRKGLDTISVTGNVLRDYLTDLFPILEIGTSAK
                     MLSVVPLLAGGGLFETGAGGSAPKHVQQFVKEGYLRWDSLGEFSALAASLEHLAAMFK
                     NEKAQILADTLDQAIAKFLDNNRSPARKVGQIDNRGSHFYLALYWALALADQSRDAEL
                     KWRFNTIASKLVANEAKINAELLAAQGRPIDLGGYYHPDFEKASRAMRPSATFNAIID
                     AAEPAVSRLQALDEVREEVLV"
BASE COUNT          534 a         1068 c         1044 g          719 t
ORIGIN      
        1 cagcagttcc ttcgtgaaac gaagcgcgta gatgcggagg tcggctcgga ggatgtactc
       61 cttcgataca ccggtgtagc gatgaacacg ctcagcgatc cgttccttct cggctttcgg
      121 cagctcgtcg ccctgaaaga gcgccaccag atactccgtt ccggcgaact tctccacttc
      181 gcgcagcagc gtgtcgaggt ccttccactg atcgggcagc ttgtggtggt agtacgccgt
      241 cgcggccaac gccggaaggg aagttgcgta cgtcaggtcg ttgccattca cgacaaacgg
      301 cagtgtcccc atgttcagcg cgggcgagag caggatgacg ccattgagac cgatgccgag
      361 gttgccctgg agtcgcggaa cgagaagggc ggagcgaatg ccgccgtagc tctcgcccag
      421 gaggtacttc ggggagttcc agcgattgtg ctgggtgatg aagctgcgga tgaactccgc
      481 gaccgagtcg gcgtcttcgt catatcccca gaaatcctcg cccttcttgt cgcccatggc
      541 gtggctgtag ccggtgccga tgggatcgat gaagacgaga tcggtggcgc ggaagatcgt
      601 gtcttcgttg tcgcgcagct tgtacggcgg gctgccggga tcggcggcgt cgctggggat
      661 gtcgatgatc ttcgggccga ccaatccgaa gtgcagccag atcgaagccg atcccggtcc
      721 gccgttgtag acgaaggtga gaggccggtc ctcctgcttc gccacgccgt ccttcgtgta
      781 ggcgatcgtg aagaagctgg cggtgttctt cccatcagca tccttcagga agatttcttc
      841 ggcggtggtg gtgtaggcga cgtccgcccc gcccagcttc atccgatgct gccggacgaa
      901 cttggccggc tcgggaggct tctcgcctgc tttgtcgcca gccttgtctg cagctttgtc
      961 tgccggtttg tccgccggct tgggatcgtc cgcacgaagc ggagcaataa acacgaatag
     1021 agcaaccaga gacgccatga acggcgcggt cagtttacgc atcacggaga ggtacctcgc
     1081 ttcctagact tggacgacgg atcgactata cgcgttagtc gggcgagggt tgcgccagta
     1141 tcattccctc tgtgaccgtc acgccggagc cccaccgcgg ccggattccg gagcttgacg
     1201 gcatccgcgg gctggccatc ctgctggtgc tggtgtggca ctacctggtt tgcgaagtcg
     1261 gcgcgccccc tccgctcttc cgtttcctga atcagacctg gagcggcgtc aacctcttct
     1321 tcgtcctctc cgggttcctc atcggcggaa tcctgctcga tcaccgcgaa gcgccgaatt
     1381 actatcgcgt gttctacatc cggcgggtct gccgcatcct gccgatctat gttgtctggc
     1441 tccttctgtt ctacatcctt cgcggaccgg tgtcgcggct cccgctcgga acggcatccg
     1501 gctggctctt cgccgatccg ctgccgttct ggtcgtacat gacgttcacc cagaactttc
     1561 tgcaggggaa gtacggcgtg tgggggccga actggctggc cgtgacctgg tcgctggcgg
     1621 tggaagagca gttctatctt ctcctgccgg tgatcgtccg gttgatgcct ccgcgcaggc
     1681 tgccatacct gctcgcccca ttcaccctgc tggcgcccgt ccttcgcttt ctcttcttcg
     1741 acccgaggat ggcggtcgat ccatcgtacg tgctactgat ctgcaaggcc gacgcactac
     1801 tcctcggcgt cttctgcgcg tggctggcgc ggcgcgaggg cgcggcggaa cggatccgcg
     1861 caaaggcgcc actgctgcgc atcctgctgc tgtcgtgtgg tggcttcgtc tgcctggccg
     1921 cgtatgtacc ggagaccgcc acgattcgtg cgctgaacga ctcgctgctc gcgatcggct
     1981 atgcctcgct cattctgcag gcgctcttca cgccgcggtc gccgtttgcg cggatggcgc
     2041 gcgtgcgctg gctgcgcgcg ttggggctca tcgcgtacgg aacgtatctg attcatcaga
     2101 ccgtcaacgg tctcgccttc ggcgcggttc gaggacattg gccctacatc acccaggcca
     2161 gcgatctcct cgtaatcctc ggcgcactaa cgatcacgat cgcagccgcg atggcttcct
     2221 ggcgctggtt cgagaagccg atcgtcgact tcgggcgccg gtggcgatac gccgatcagg
     2281 cgtcttccga ctgacttaga ccagcacctc ttcccgaacc tcatcgagtg cttgcagccg
     2341 cgatacggca ggctccgctg cgtcgatgat cgcgttgaag gtcgcgctcg gcctcatcgc
     2401 tcgcgacgcc ttttcgaagt cgggatggta gtagccgccg aggtcgatgg ggcgtccctg
     2461 cgcggcaaga agctcggcgt tgatcttcgc ctcgttggcg accaacttac tggcgattgt
     2521 gttgaacctc cacttcagct ccgcgtcgcg cgactggtca gccagcgcga gagcccagta
     2581 caacgcgaga tagaagtggc tgccgcgatt gtcgatctgg cccaccttgc gcgccggcga
     2641 gcggttgttg tcgaggaact tcgcgatcgc ctgatcgagc gtgtccgcaa ggatctgtgc
     2701 cttctcgttc ttgaacattg cggcgagatg ctcgagggac gcggcaagcg cagagaactc
     2761 gccgagcgaa tcccagcgga ggtagccctc tttgacgaat tgctggacgt gcttcggtgc
     2821 agaaccgccg gcgcccgtct cgaaaagacc tcctccggcc agtagcggca cgacggagag
     2881 catcttcgcg ctggtgccga tctcgagaat cgggaaaagg tcggtgaggt aatcgcgcag
     2941 gacgttgccg gtcaccgaga tcgtatccag ccccttccgg attcgctcca gcgagtagcg
     3001 catcgcctgc accggagcca tgatccttat ctccaatccc tcggtgtcca tagttcccag
     3061 cgactgcacg accttcgtga tgacgttcgc gtcatgcgcg cggttgctgt cgagccagaa
     3121 gacggcgggc gcgctggttg tccttgcccg ccggacggcc agcttcaccc aatcctgtat
     3181 cggaatgtcc ttggcccgcg acattctcca gacgtcgcct gcttcgacct tgtgccgcat
     3241 cagcacgttg ccgtttgcat cgacgacacg cacgactcct tcgaagggga tctcgaacgt
     3301 cgtcgggtgc gatccgtact cttccgcctt ctgcgccatg aggccgacgt tcggcacgga
     3361 gccca
//
LOCUS       DATLVW010000208         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_586611, whole genome shotgun sequence.
ACCESSION   DATLVW010000208 DATLVW010000000
VERSION     DATLVW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586611"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            509..1963
                     /locus_tag="VNN25_06750"
     CDS             509..1963
                     /locus_tag="VNN25_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH91262.1"
                     /translation="MAATRLRIFVSSVQKEFAEVRANLKTFLLGDAVLRRFIAEVFLF
                     EELPASDRRADEVYLAEVDRSDIYLGIFGNDYGFEDAEGIAPTEREYDRATQKNKTRL
                     IYVWGSDDDARAPKMRALIKKASSELIRRRIEDGSALTAEVYASLVDHLDRRGALRIP
                     PFDTSAADRTSIASLSRKRISWFLETASRERNFPMTPKTSTKALLTHLNLLSDGKPTN
                     AAILLFGAEPQRVHRTAETKCVQSHGTEYQRPFVSYQIYGGDVFEQADQARDFVLSRI
                     NRSVGTRSESITATAEYELPRDAVAEAIINAIVHRDYNSNASVEVRLFSDRLEVWNPG
                     VLPGTLTTENLREDHASVPNNPLIADPMYLARYIEKAGTGTQLMISLCLNAGLPEPDF
                     EERSGSIVVTLWRDWLTNEVMNALQLNDRQVTGLALLRADGKITRGKYQVSTGAGRTT
                     AARDLEDMMKKGVIERRGERRGSFYVKSKGMTHK"
BASE COUNT          485 a          623 c          622 g          402 t
ORIGIN      
        1 gaaatgctaa tcgaaaagtt gggcatgttg ctcttccctc tctttctcta tgttgcgggc
       61 aagtcgtgag cggcttcgaa cggcggggaa ccggagcact acggtggcgc gatggagcta
      121 aggcactgag cgtaaaacgc tgcaccaaat cactgaagta tgggtggaga gctaccgtgc
      181 tgggtttcca gcaacatatg ccgggccgag ggaaccgtca agttccttgg aaggccggtt
      241 ccgccggact cctcccgtca caaatgcgcg gatctgctta ccctacattg actcggtacc
      301 cgcccccacg gtcgcgggtg gggcatggtg aacccaccat cgtcgccttg atcgcggagc
      361 gcggccatga tggcttgatc gcgaagaaca ccaaacggag gggacagtcg aaagtgaaag
      421 tgacgacgaa tggcttcggg gcgacccgac accaagctcg tcactttcac tttcgacctg
      481 acatctccgc tgccatactg aatcgctgat ggctgccaca cgtctccgga tcttcgtcag
      541 cagtgtccag aaggagttcg ccgaggttcg tgcgaatctc aagaccttcc ttctcggcga
      601 cgcagttctg cgacgtttca tcgccgaggt ttttctcttt gaggaattgc cggcgagcga
      661 ccggcgcgcg gacgaggtct acctcgctga agtggatcgc tccgatatct atctcgggat
      721 cttcggaaat gattacggat tcgaagatgc ggaagggatc gcacccaccg aacgggagta
      781 cgaccgggcc acgcagaaga acaagactcg cctaatttac gtctggggat ccgacgacga
      841 cgcccgcgct ccgaagatgc gagccttgat caagaaagcc agcagcgagc tcattcggcg
      901 acgaatcgaa gacggcagcg cgttgacggc ggaggtctac gccagtcttg tcgatcatct
      961 cgaccgtcgt ggtgcgctcc gcatcccacc cttcgatacc tcggcggcgg accggacctc
     1021 gatcgcaagc ttatcccgca agcggatctc gtggtttctc gagacggcgt cacgcgagcg
     1081 caacttccca atgactccga agacaagcac aaaggcgctt ctgacccatc tcaatctact
     1141 gagcgacgga aaaccgacca atgctgcgat tctcctcttc ggagctgagc ctcagcgagt
     1201 ccatcgaacc gcagaaacca agtgtgtgca gagccacggc acggagtacc aacggccgtt
     1261 cgtttcttac cagatctacg gtggcgacgt gttcgaacag gcggatcagg cgcgcgactt
     1321 tgtgctctct cgaatcaacc gaagcgtcgg cacgcggagc gagtccatca ccgctacagc
     1381 ggaatacgaa ctgccccgtg atgctgttgc cgaagcgatc atcaacgcca tcgttcaccg
     1441 cgactacaac agcaacgcct cggtggaagt gcgcctgttt tccgatcgtc tcgaggtctg
     1501 gaatccaggc gtcctccccg gcacattgac cacagaaaat cttcgcgagg atcacgcctc
     1561 ggtgccgaac aatcccctga tcgccgatcc gatgtacctg gcacgctaca tcgagaaggc
     1621 aggcaccggc acgcaactga tgatctccct ctgcctcaat gcagggctgc ccgagcctga
     1681 cttcgaagag cggagcggat cgatcgtcgt gaccctctgg cgcgactggc tgacgaatga
     1741 agtaatgaac gcgcttcagc tcaacgatcg gcaggtcacc ggtcttgcgt tgctgcgagc
     1801 agatggaaaa attacgagag gaaaatatca agtgagcacg ggtgcaggga gaacgaccgc
     1861 tgctcgtgat ctggaggaca tgatgaagaa gggcgtcatc gaacgacgtg gggagcggcg
     1921 tggaagcttc tacgtcaaga gcaagggaat gacccataaa tgacccatat gacccaggac
     1981 tccacctgcg aagatgcctc ggggatggcc ggcaggtcga cgagggcggg ggcaacaaag
     2041 cccagggaat gactctgaac taatggatta ggtaataacg atgaaggcct cctacctggc
     2101 ttcacgcatg gcgcggatct ctcgctgact cc
//
LOCUS       DATLVW010000209         4873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_586797, whole genome shotgun sequence.
ACCESSION   DATLVW010000209 DATLVW010000000
VERSION     DATLVW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586797"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNN25_06755"
     CDS             <1..518
                     /locus_tag="VNN25_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXH91263.1"
                     /translation="ELLRDEGKSVGLLDGRYVQTEIDRVAELPDNDAFGAKTGPIYIS
                     LFQNYLRNDLGVDLVKRYAPQNGEANQKWKRPANGRGAFAGFIDVTGALAQGTKDNEA
                     LRVFAAAGYNDLTTSYFAMRYMLEHSGIDINRLTIKDYPGGHMMYLHRPSLEALSKDI
                     VTFIEKGNGGH"
     gene            827..3448
                     /locus_tag="VNN25_06760"
     CDS             827..3448
                     /locus_tag="VNN25_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH91264.1"
                     /translation="MSKVDIADVFARADAAIGNAPPEARRIILEAGELDRKSEHFIPI
                     QQILARSFYKDEDLRAPQKFESALEILETHCVLSGSANGETLGLAGAIYKRMWDYTGQ
                     RTHLEKSLKWYAKGAEVATDADGWLAVNVAFVSDLLGAQEGDAGGPDADVRKKARFYY
                     ADNVRKAVLARLNAAGPPKGKNPWYYHASRAEAAFGLADFAAAAAALDEGRSAIAALP
                     KEKAAAETTARQLALLAHLRFEDTPHYDVATELIKQSFRINNHAVSALFGGKIGLALS
                     GGGFRASLFHIGVLAKLAELDLLRHVEVVSCVSGGSIIGAFYYLELKKLLESGKTTHG
                     DYLQLVRDVERKFLEGVQKNVRMRIAASPRAGFKMALQSGYSRSDVIAELYQEHFYDQ
                     TAAEGKEPARLMSDLIVHPAEYGGAPDDFTPKYDNWRLGAKVPMLVLNATTMNTGHNW
                     QFTATWMGESPQAISEAIDAIPRYRRAYYKEAPKPHDEIPIGRAVGASACVPALFEPI
                     VLRNLYPDRTVRLVDGGVHDNQGLSSLLEQNCRVILVSDSSGQFQEQASPGGSVSDSL
                     GNSNGVLQARVREAQFDQLSALRRAGAIQGAMFIHLTKDLEAAPVSWIGGSEKPAENP
                     DPVTCYGIDREIQRKLAAVRTDLDSFSDAEAYALMTSGYRMTEHALIKEKSLPSLQPT
                     GSRQQWRFLAVEDALTRTDPQGRERMLTLLSVSNELALKVWKQLPPLKALAAGVGGAA
                     IIGAGYALWKKRMVELPSLGVVSLVAIALLAGKGILAQLDKRRIYHKRLGEMGLGFAI
                     AFVGWIVAGLHLLIFDRLFLDHGKWDAGTKQPAAGMAPPGPHIPVANNQPPGKASGGG
                     VLSVPEAAGERPEHTVN"
     gene            3717..>4873
                     /locus_tag="VNN25_06765"
     CDS             3717..>4873
                     /locus_tag="VNN25_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH91265.1"
                     /translation="MTFGQGSDRWRSTGSGGLLAKIVFFAGASALVLMVIPTSVLQSI
                     SSLGFKSKATTRTIETRVSGERAWAPFRTSPPAELSKSALASVIGAASGNVRGTEAPA
                     DRHAAGVAQLLAGHPRAALPILTAEAEKSNRGEAWIDLAAVLHETAMHEDAPELLAES
                     LAASDRALSLQPQSPEALFNRAIVIEHLGLRDDAREAWERYLALDSISDWADEARTHR
                     DGLSPEEAFLDRLDRQYDRVATDPALAMALTRSDPFGARGMAAKEVLGRWGKATLLHD
                     ERKADLHLRVARQLGAAVAAVQGDRMLERAVAAIDASSGTKRSLLASAHSEYSDGLQV
                     YAANRPGDAELLLRRAAAAFETARSPMILPARLFAANMVYEQGHHDESERE"
BASE COUNT          932 a         1521 c         1569 g          851 t
ORIGIN      
        1 aggaactgct gcgcgacgaa gggaagtcgg tgggcctcct cgacggacgc tacgtacaga
       61 ccgagatcga ccgcgtggcg gagttacccg acaatgatgc gttcggcgcc aagaccggac
      121 cgatctacat ctcactcttc cagaactatc ttcgcaacga cctcggcgtc gatctggtga
      181 agcgatacgc gccgcagaac ggggaggcaa accagaagtg gaagcgtccc gcaaacggac
      241 gcggcgcctt cgccggcttc atcgacgtca ccggcgcgct ggcccagggg acgaaggaca
      301 acgaagcgct gcgcgtcttc gccgccgccg gctacaacga cctgacgacg tcgtacttcg
      361 cgatgcgcta catgctcgag cacagcggta tcgacatcaa tcgcctgacg atcaaggact
      421 atcccggcgg ccacatgatg tatctccacc gcccatcgct cgaagcgctg tcaaaggaca
      481 ttgtgacgtt catcgagaag gggaacgggg gacactaggc gcgccggcga gctggccttt
      541 ttttgaccac ggagacacag aggtcgcaga gtacgtttca cctctgcgtc ctcggtgtct
      601 ctgtggttca ccccgaaaaa cacgtcagct caccgctgcg atcagctcct cgatccacgg
      661 cacgtcgccg ccgcgctgct tacgcgattc ggcgatgatg aggtgcagat cctcgccaca
      721 gcggagtcat ccgtccgtcc atccataact gttgccgtcg cctcgaaacg gacctatctt
      781 tgatggatcc ccacaagttg tcgtcactcc gggacggagg tattcgatgt cgaaagtcga
      841 tattgctgac gtattcgcca gggccgacgc tgcaatcgga aacgcgcccc ccgaggctcg
      901 taggatcatc ctcgaggcgg gcgagctgga tcgcaagagc gagcacttca tccctatcca
      961 gcagatcctc gcgcggtcgt tttacaaaga tgaggacctg cgcgcgccgc agaagtttga
     1021 gagcgcgctc gagattctgg agacccattg cgttctcagc ggctcggcaa acggtgagac
     1081 cctcggtctc gctggtgcga tttacaagcg catgtgggac tacaccggcc agcgaaccca
     1141 cctggagaag tcgctgaagt ggtatgcgaa gggagctgag gtggcgactg acgccgatgg
     1201 ttggctcgcc gtgaacgtcg ccttcgtatc cgacctcctc ggcgcgcagg aaggcgacgc
     1261 cggcggcccc gacgcggatg tgcgcaagaa ggcgcgcttc tactacgcgg ataacgtacg
     1321 caaggcggtg ttggcaaggt tgaacgcggc cggtccgccg aaaggcaaaa acccgtggta
     1381 ctaccacgca tcgcgcgccg aagcagcgtt cgggctcgcg gatttcgcgg cagccgccgc
     1441 agcgctcgac gagggacgca gtgcgatcgc cgcgttgccc aaggagaagg cggcggcgga
     1501 gaccacggcg cgccaactcg ctctcctcgc gcatctccgc ttcgaagaca cgccgcatta
     1561 cgacgtcgcg acggagctca tcaagcaatc gttccgcatc aacaatcacg ccgtgagcgc
     1621 gttgttcggc gggaagatcg gcctcgcgct ttcgggcggt ggctttcgag cgtcgctgtt
     1681 tcacatcggc gtgctggcca agcttgccga gctcgatctc cttcgccacg tcgaggtggt
     1741 gtcctgcgtc tccggaggct cgatcatcgg cgcgttctac tatctcgagc tcaagaagct
     1801 cctcgagagc ggaaagacca cgcacggcga ctaccttcag ctcgtgcgcg acgtcgagag
     1861 gaagttcctc gaaggcgtcc agaagaacgt ccgcatgcgc atcgccgcgt cgccgcgtgc
     1921 aggtttcaag atggcgctgc aatcgggcta ctcgcgctcg gacgtcatcg cggagctcta
     1981 ccaggagcac ttctacgatc agacggcggc ggaagggaag gagcctgcgc gcctgatgtc
     2041 cgatctcatc gttcatcccg cggagtacgg cggagcaccg gacgatttca cgccgaagta
     2101 cgacaactgg cgcctcggcg cgaaggtgcc gatgctcgtg ctcaacgcca cgacgatgaa
     2161 cacggggcat aactggcagt tcaccgcgac gtggatgggc gagtcgccgc aggccatcag
     2221 cgaagcgatc gatgccatcc cgcgataccg gcgcgcgtat tacaaggaag cgccgaaacc
     2281 gcatgacgag attccgatcg gccgcgcggt cggcgcctcg gcgtgtgtgc ccgcgctgtt
     2341 cgagccgatc gtgctgcgaa atctttatcc cgaccgcacg gtgcgtctcg tcgacggcgg
     2401 cgtgcacgac aaccagggtc tgtcgagcct tctcgaacag aactgccgcg taatactcgt
     2461 cagcgattcc agcggccagt ttcaggagca ggcgagtccg ggcggaagtg tcagcgactc
     2521 tctcggcaac tcgaacggcg tgctgcaggc gcgcgtgcgc gaggcgcagt tcgatcagct
     2581 cagcgcgctc cgccgcgccg gcgcgattca gggggcgatg ttcattcacc tgacaaaaga
     2641 tctcgaagcg gctccggtgt cgtggattgg cggatcagaa aagcccgcgg agaatccgga
     2701 ccccgtgacc tgctacggaa tcgatcgcga gatccagcgc aagctcgcag cggtgcgcac
     2761 ggacctcgat tcgttctccg acgccgaggc atatgcgctg atgacgagcg gctaccggat
     2821 gacggagcat gcactgataa aggagaagag cctgccatcg ctgcagccca ccggatcgcg
     2881 ccagcagtgg cggtttctcg cagtcgagga cgcgctgacc cgcaccgacc cgcagggcag
     2941 ggagcgcatg ctcactctgc tcagcgtgtc caacgagctg gcattgaagg tttggaagca
     3001 actgccgccg ttgaaggcgc tggccgcagg ggttggtggc gccgcgatca tcggcgccgg
     3061 ttacgcattg tggaagaagc gcatggtcga gctgccgtca cttggagttg tctccctggt
     3121 tgccatcgca ctgttggcgg gcaaagggat cctcgcgcag ctcgacaagc gtcgcatcta
     3181 tcacaagcgc ctcggcgaga tgggcctcgg cttcgccatt gccttcgtcg ggtggatcgt
     3241 tgcggggctt catctcctga ttttcgatcg tttgtttctc gatcacggaa agtgggatgc
     3301 cggaacgaag cagcctgcgg cggggatggc ccctccgggg ccgcacattc ccgtggcgaa
     3361 caaccaaccg cccggcaaag cgagcggcgg aggcgtcctg tcagtacccg aggcggcggg
     3421 cgagcgaccg gagcacaccg tcaattgaga tccgactgaa aaccgtttca cgcggagggc
     3481 gaggacgcgg ggggttctcc gcatctccgc gtgacaagcc tcgggatccc gatgcaatcg
     3541 atcaaaataa tcagcaacct attggtccct tcgatgtatc cttacacctt ctcccaacat
     3601 tcgaagctgg ccgtaccgat ccgcgtgcga gcggtcgtcg cgaattcgcg cggaaaccat
     3661 tggcggcggc gtacgtctac catatcggga gaattgcagg ggcgggagat gcacccgtga
     3721 cattcggaca aggaagcgat cgatggcgct cgaccgggag cggcggtctc ctggcgaaga
     3781 tcgtcttttt cgcgggagcg agcgctctcg tgctgatggt cattccgacc tcggttctgc
     3841 agtcgatttc ctccctcggc ttcaagtcaa aagcaaccac gcgtacgatc gagactcgcg
     3901 tgagcggcga gcgcgcctgg gcgccgttcc gtacgtcgcc tccggccgag ttgtcgaaga
     3961 gcgccctggc gtcggtgatt ggagcggcga gcgggaacgt tcgcggaacc gaagcgccag
     4021 ccgaccgcca cgccgcggga gttgctcaac ttctcgccgg gcaccccagg gcggcgctgc
     4081 cgattctgac ggcggaagcg gagaagtcga atcgcgggga ggcttggatc gatctggccg
     4141 cggtacttca tgagacagcg atgcacgagg atgctcccga gcttctggcg gagtcgcttg
     4201 ccgcgtccga tcgagccctt tccctacagc ctcagtctcc cgaggctctc ttcaatcgag
     4261 cgatcgtcat cgagcatctc gggttgaggg atgacgcccg cgaagcctgg gagcgatacc
     4321 ttgcgctcga ctccatcagc gactgggctg acgaagcccg gacacaccgc gatgggctgt
     4381 cgccggaaga ggcctttctc gaccggctcg accgccagta cgaccgagtg gcaaccgacc
     4441 ccgcgctggc gatggcgctg actcggagtg atcccttcgg tgcacgagga atggcggcca
     4501 aggaagtcct cgggcgatgg gggaaggcta cgttgctcca cgacgagcga aaggcggatc
     4561 ttcatctgcg cgtcgcacgg caactcggag cggctgtggc tgcggtccaa ggcgaccgaa
     4621 tgctcgaacg agccgtcgct gccatcgacg cgtccagcgg cacgaaacgc tcgctgttgg
     4681 cgtctgcgca ttctgaatat agcgatggtc tgcaagtgta tgcagcgaat cgcccggggg
     4741 atgccgaact gctgctccgc cgcgccgcgg cagcattcga gacggcgcgg agtccgatga
     4801 tcctgccggc gcgtctcttc gccgcaaaca tggtttatga acagggccac cacgacgagt
     4861 cggagcgcga gat
//
LOCUS       DATLVW010000210         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_586824, whole genome shotgun sequence.
ACCESSION   DATLVW010000210 DATLVW010000000
VERSION     DATLVW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586824"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..613)
                     /locus_tag="VNN25_06770"
     CDS             complement(41..613)
                     /locus_tag="VNN25_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH91266.1"
                     /translation="MSVPLHHSNFTYADYLAIEASSNIKHEFLGGQIYAMAGGTPEHA
                     ALAAAAIGLLFAQLRGGNCRVYDADLRVRTPSGLATYPDITIICGPSVRDEDDLQAVT
                     NPTLIIEVLSRSTEDYDTGDKFEHYKTLPSVKQYVLVSHRDRSVETWTRENDNHWRSA
                     IVREGAAAELLVGAHLDVHELYEAAAEPRV"
     gene            738..>3062
                     /locus_tag="VNN25_06775"
     CDS             738..>3062
                     /locus_tag="VNN25_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH91267.1"
                     /translation="MKRAIAALVCSLSFFTVSLFGQTSASLTGIVTAEGRALPGVVVT
                     ISSPELQGTRQTITGDGGAYSFSALPPGEYAISMTFGGMETQRLRSSLRLSQTSRVDA
                     VLKPAGTSEAITVTAAAPSVIESPQISTNMTLPFIERLPIQRNQLATAQFAPGVNGNT
                     LANGELYISGGPGYDNLVLVNGVAVTEQTRGQMRPMYVEDAIQETTVLTGAISAEYGR
                     FSGGVVNSITKSGGNELAGSLRDSLSNPAWVAQSPALEARENNLNHVGEGTLGGFLIR
                     DHLWFFGAGRLAKNDTSRQTVSIPASTTNPISPASPAISYAEGNDQKRYEAKLTSQIG
                     ARQTLAASWFGVNTKGTNARFSANLYDEASLTTRTDPESLVAVHYNGMAATNLLLEGH
                     YSARKFSDRSGSPTTDLIAGTVLLDRNNNNTRFHSPSLCEICDAERRNNKDVLLEAHD
                     FFDAKSFGSHDLVAGVDRFTESRYANNHQSGSDFSIFVTKAQYSNGVIYPVITPSNAN
                     GGGTFIRWTPILTPASANDLRTDSAFVNDTWSATSRLTLMIGARFDRNHAVDADGTVS
                     SDDSKLSPRLSAQYDLRGDGRHRFSVSFGEYASRIADNIASSNQAAGNAAAIDFAYKG
                     PAINNTSLTVPMDDAIRRIFDFFNSTQGGTANRTAANLRANGSRTIPGFATYFDGSLA
                     SPSVREFTTGYGAQIGRNGFVRADYVHRDWRDFYAASVTTATQKVNTPLGIPVDLQLV
                     RDSNNMVRQYRGVQLQARWTPGRFDFGTHYTWSKL"
BASE COUNT          565 a          974 c          950 g          573 t
ORIGIN      
        1 gcggatgagg gctggcgcgg cctctgccct ctcgcaaggt tcacaccctc ggctcggccg
       61 cagcctcgta gagctcgtga acatcgaggt gagcgccgac cagcaattcc gcggccgcgc
      121 cttctcgcac gatggctgag cgccagtggt tgtcgttttc gcgtgtccag gtttcgacgg
      181 aacggtcgcg gtgcgacacg aggacgtact gcttcacaga cggaagcgtc ttgtaatgct
      241 cgaacttgtc gcccgtgtcg taatcctcgg tgctccggct caggacctcg atgatcagcg
      301 tgggattggt gaccgcctgc aggtcgtctt catcgcgcac gctgggcccg cagatgatcg
      361 tgatgtccgg atacgtggcc agaccggacg gcgtgcgcac gcgcagatcg gcgtcgtaca
      421 cgcggcagtt gccgccgcga agttgcgcaa agagcaatcc gatcgccgca gcggcgaggg
      481 ctgcgtgctc cggcgtgcca ccggccatcg cgtagatctg gccgccgagg aactcgtgct
      541 tgatgttgct cgaggcctcg attgcgaggt aatccgcata cgtgaagttg ctgtggtgaa
      601 gtggtacgga catcgagacg aattatcgca tgcgagcaag atcttggggt ggcgccggtt
      661 gctcccgaaa tcttcgtctg gcagcggcaa cgcgttatca tcgatccatc cttcaattca
      721 tcgggagagg atcgacaatg aagagagcca tcgcggcgtt ggtttgctcg ctttcattct
      781 tcacggtttc gcttttcggc cagaccagcg cttcgctcac gggtatcgtg actgccgagg
      841 ggcgggcgtt gccgggcgtg gtggtgacga tctcctcgcc ggagttgcag gggacgcgac
      901 agaccatcac tggcgacggc ggcgcgtaca gcttttccgc gttgccgcca ggcgagtacg
      961 cgatctcgat gacgtttggc ggcatggaga cgcagcggtt gcgttcgtcg ctccggcttt
     1021 cgcagacatc gcgcgtcgat gcggtgctga agccggccgg gacgagcgag gcgattactg
     1081 ttaccgccgc agcgccctcg gtgatcgaga gtccgcagat ctccacgaac atgacgctgc
     1141 cgttcatcga gcggctgccg attcagcgta atcagcttgc aaccgcgcag ttcgcgccgg
     1201 gcgtcaacgg caacacgctc gcgaatgggg agctctacat ctccggaggt cccggatacg
     1261 acaacctcgt gctggtcaac ggcgtcgctg tcacggagca gacgcgcggg cagatgcggc
     1321 cgatgtatgt cgaggatgcc atccaggaga cgaccgtatt gaccggcgcg atctcggcgg
     1381 agtacggccg gttcagtggc ggcgtcgtca acagcatcac gaagtcgggt ggcaatgagt
     1441 tagccggctc gttgcgcgat tcgctcagta atccggcgtg ggtggcgcag tcgccggcgc
     1501 tggaagcgcg cgagaacaac ctcaatcacg tcggcgaagg gacgctcggt ggcttcctca
     1561 ttcgcgatca tctctggttc ttcggcgcgg gccggctggc aaagaacgac acgtcgcggc
     1621 agacggtctc cattccggca tcgacgacga atccgatctc gcccgcatcg ccggcgatca
     1681 gttatgccga ggggaatgat cagaagagat acgaagcgaa actgacgtcg cagattggtg
     1741 cgcggcaaac gctggccgcg tcatggttcg gcgtcaacac gaaaggaacc aacgcgcgct
     1801 tctcggcgaa tctttacgac gaggccagtc tcaccacgcg caccgatccc gagtcgcttg
     1861 tggccgtcca ttacaacggc atggccgcca cgaacctgct gctggaaggc cactactcgg
     1921 cgcgcaagtt ctccgaccgg agcggttcgc cgactacaga tctgatcgcg ggcaccgtcc
     1981 ttctcgaccg caataacaac aacacgcgct tccactcgcc gtcgctctgc gagatctgcg
     2041 acgccgagcg ccgcaacaac aaggatgttc tgctcgaggc tcacgacttc ttcgatgcga
     2101 agtcgttcgg cagccacgac ctcgtcgccg gtgtcgaccg attcaccgag agccgctacg
     2161 cgaacaatca tcaatcgggc agcgacttct cgatcttcgt cacgaaggcg caatacagca
     2221 acggcgtgat ctatcccgtc atcaccccgt cgaacgcgaa cggcggcggc accttcatcc
     2281 gctggacgcc gatcctcacc cccgcgagcg cgaacgacct gcgcaccgat tccgcgttcg
     2341 ttaacgacac gtggagcgcg acgtcgcgcc tgacgctgat gatcggcgca cgattcgacc
     2401 gcaatcacgc tgtggatgcc gatggcacag tcagctcgga tgattcgaaa ctctcgccgc
     2461 ggctctccgc gcaatacgat cttcgcggcg acggacggca tcgattcagc gtttcgttcg
     2521 gcgagtacgc ctcgcgcatc gcggacaata tcgcgtcgtc gaatcaggcg gccggcaatg
     2581 ctgccgccat cgatttcgct tacaaaggtc cggccatcaa caacacgtcg ctgaccgtgc
     2641 cgatggatga cgcgatccgg cgcatcttcg atttctttaa cagcacgcag ggcggcacgg
     2701 ccaaccgtac cgcggcgaat ctgcgcgcga acggctcgcg cacgattccc ggcttcgcca
     2761 cctatttcga cggatcgctc gcctctccct cggttcgcga attcacgacg ggatacggcg
     2821 cgcagatcgg caggaacggt ttcgttcgtg ccgattacgt tcatcgcgac tggcgtgatt
     2881 tctacgccgc cagcgtcacc accgccacgc aaaaagtgaa cacgcccctc ggcatccccg
     2941 tcgatctgca gctcgtccgc gacagcaaca acatggtgcg ccagtatcgc ggcgtgcagt
     3001 tgcaggcgcg ctggacgccg ggccgcttcg acttcggcac gcactacacc tggtcgaagt
     3061 tg
//
LOCUS       DATLVW010000211        10989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_544278, whole genome shotgun sequence.
ACCESSION   DATLVW010000211 DATLVW010000000
VERSION     DATLVW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544278"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3450)
                     /locus_tag="VNN25_06780"
     CDS             complement(<1..3450)
                     /locus_tag="VNN25_06780"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXH91268.1"
                     /translation="MKAAALAFLSVLAATAAVAGEVNIHVLAIGVKGYPNYPADHRLE
                     YTDRDAAAFADFWTRQAAANGLSVHVTKLLGGEATRGEIHKAIQSLDTARPDDLVFIF
                     FAGHGEMERDDAYLMPYEASNTAPAAYGYRVDEFIHELRKWISARHVVIFIDACHAGG
                     AIPDAGSRGDNGNITERFRSVWKEQLLGTDSTFVAFLSAGSREVAWEDPALRHGVFTH
                     YLLEGLAGAADGKPFGDGNGIVTTGELYEFLRRQVAERTASAFPSKQTPVISPNYNEK
                     LPLTVLRKPSAGAPAEAPLRADSGVVDTKFPLLPRDDGAPAAPSGEAATLLKKARTVV
                     ESDDLFCFHHPVENDLLSWLYYGQIAAYDAANQSLKIEESPWGHFYRGVALARLRAFE
                     PALADLTKAEEGFREVKAEELATRTARMKDLIFQWRRLPAATQQPATNACSVDASRTV
                     CELSERFEHPEEAAAPPPASVVDKLAALLDAPRCRDCITAAMSPQIECGEWIASTLTE
                     AAQAARAYDVLLKAALREGQVRDAARFARERYLRLIVIGRRDEARQVATAHGALWDDF
                     DLSRIALAEAFDTSASDRDFETNVVITRLDAATKRSITDVRAAARRHHAALEVLSKSL
                     VEAAAPIDSSEPAVVKALSEGEGRIPLQRLEWLLKADAVSVQELSAARRALFAAISDD
                     FARLPSAPTKLPRLLALTRRVSEIPHQYLRYAEIDFTPGAAEKVVEMLYSVALDYAIR
                     SGRDPQQYILNADDFLPAITAGYRPVAVAVSSGLRRDDNDKVAFQLAQRIDAEDWFQD
                     TAGFTQIVMKVYPCLFVPHRDVSKCFTDAAWSMLDLAHLAPQFKAVTDANERVKQQGA
                     QEFTSGATGDELVLWRHLRLIANELPKVLAEGNYTDVQRFVAAAVYIGIHEGGDIAKV
                     SGLPAHEGSDGTDLQKSPSMDAQAKELISAFYLLWQSAHTDPSPDLGARARTLAIRAR
                     NPIARVLAMAVPGVYLGMSRYEIRSFLALMNVGPAEPNQLSLPHFAVLAAMIDAALKK
                     PQLKYDALRTLAEYLPDDDGPADYVAWVPVDPNHETIAAAMQPAGYPSAGGCRHIDMN
                     VGAKDEGDDLLVIIHVACVSVVQMKWSLLSIAAAREQRWDEVAAALDVLEARGNAPFG
                     ARHL"
     gene            complement(3447..3863)
                     /locus_tag="VNN25_06785"
     CDS             complement(3447..3863)
                     /locus_tag="VNN25_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91269.1"
                     /translation="MAIRDQRRVLLFLWFGGFASLLVYIYALYLADWIGPESFAGALS
                     TLSAAFGPYLGAMLTFFWTAAAPAKKAGPDPKTAWAAALCGSALWIASVAALLLQPLL
                     GHVSMEETLPRIVSTTAFLSWIVAGAIGYYFAASQK"
     gene            complement(3863..4567)
                     /locus_tag="VNN25_06790"
     CDS             complement(3863..4567)
                     /locus_tag="VNN25_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91270.1"
                     /translation="MFELRSLGHSTAAATIVTLSLFASDLRFHATAVPDSGTVRLTWS
                     APGCSSAYVTGAGVRAADSEAVIHASLDGEYTLVADCGGAVRTSSAKARYRGARGMFE
                     EWPTLADFHAANEQRRTVQNASRFAEKLHDVLQGALGFSVYEHLLPDGRLRFLTNPRE
                     LPSGRQTSETNIRNRRTAFLVDVTFPRRVPGTALIAISTAIQTRRKLESTWRPESSDD
                     IFREHEHRLVALLPAE"
     gene            complement(4815..4890)
                     /locus_tag="VNN25_06795"
     tRNA            complement(4815..4890)
                     /locus_tag="VNN25_06795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4854..4856),aa:Glu,seq:ctc)
     gene            complement(5032..5658)
                     /locus_tag="VNN25_06800"
     CDS             complement(5032..5658)
                     /locus_tag="VNN25_06800"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HXH91271.1"
                     /translation="MTTLLYFHGFASSPASAKITALRPMLEPEIELITPDLNVPSFEA
                     LDWNAIVEHAMNVAHAHPPDLIAGSSMGALVALAIAQRGIIAPMVLIAPALGIADRWR
                     TLLPDGDPILVFNHARNAEVPIHRKFFEQMFQVRVDRNPPPSPVTVIMGRNDESVPFD
                     LVENTWRQWQPSLAPSSKFIAIEEGDHGLTAHAALISGEIRAAIPGRS"
     gene            complement(5701..6372)
                     /locus_tag="VNN25_06805"
     CDS             complement(5701..6372)
                     /locus_tag="VNN25_06805"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HXH91272.1"
                     /translation="MNKRNAIKLAMLLVLAGGVAALYFTPLRAYLTKAHINDFIAWLR
                     GLWYGPIVLIVLYAAGCVFALPASIFVIAAGVIWGWKLGGLYAIIGGFLGAIAAYYVG
                     GFLGEGLLDKFGSVGRGVRKQVESSGFLSMLIVRLIPGPPFAVWNYAAGVAKMRFRDY
                     ALATLIGIIPSHLVFTYCADSLVNGTMTQGDALKRLAIVCALLLALILIPLYIKKRMG
                     VKPIE"
     gene            complement(6590..7483)
                     /locus_tag="VNN25_06810"
     CDS             complement(6590..7483)
                     /locus_tag="VNN25_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91273.1"
                     /translation="MRVGTLASRRLARRRPAAAFCVGLLFLIAAANARAVETVRIAPV
                     INAPAGVVVHGTAELHIAGTSTIRDLDSTVLTTKRIASADREEYSDTTLSRDAAGSRF
                     RRVYGRVIHEEESGNTFGVMNGRSLLFVMKGRNVTVTPDTGPDLTVDETMGLTELAQR
                     ALRVESANFCIAPYALTVGASWNVPASLLADCYDELGHVVKTIPGRGTLRAIENQDGH
                     RVAIIELSFVRSIDSMGEIKFDSLADAAVTSKIEITLDQPVKWRRLTLTTLSGVSHPK
                     GLAKPSVTIALQATETVTSAP"
     gene            complement(7483..8652)
                     /locus_tag="VNN25_06815"
     CDS             complement(7483..8652)
                     /locus_tag="VNN25_06815"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXH91274.1"
                     /translation="MKDIVILDGARTPMAEYNGAFSDISAIDLAVVAAKEALSRSGVA
                     PEEVNHVIVGNALQTSGDAIYGARHVGLKAGVPKEVPALTLNRLCGSGIQSIISAAEQ
                     IQLDEATTILAGGMENMSQAPHVIRGARKGLKLGQGALEDSLMVALLDSYVGLYMAQT
                     SDNLARKYSISREEQDQFALQSQKRAAEAESAGRLKEEIVAVAVGRKGELFTSDDHLR
                     PDTTMEGLAKLKPAFAKDGFVTAGNASGIVDGAAMVVVTSADNAKNKKPIGRIVSWGI
                     AGCAPDIMGIGPVPSTKIALKKAGMSLNDIDLIEINEAFAGQILAVSKELGIDTAKLN
                     VNGGAIALGHPLAASGTRLVITLLYELRRRQKRFGLASACIGGGQGIAIIVESIQ"
     gene            complement(8892..10988)
                     /locus_tag="VNN25_06820"
     CDS             complement(8892..10988)
                     /locus_tag="VNN25_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH91275.1"
                     /translation="MIAFAASTAPIDYPKTKKVEQVDDYHGVKVADPYRWLENDVRTS
                     KDVADWVAAENKITFDYLGAIPEREPIRKQLTTLWNYEKYTVPFKRGSRYFFSKNNGL
                     QNQSVVYTVDKWTDTPRVLLDPNTWSKDGTVALGGLAISDDAKYAAYATAEAGSDWEV
                     IRVADVATSQPLSDEIKWVKFSGISWTKDDKGFFYSRFPAVEAGKAYQSLNRDQKVYY
                     HRLGTPQSDDVVVWYDASQPEWNYGAEVSEDGRYLIITTTQGTAAKNQVHVKDLQDPY
                     AMPVAVVDKIENDYSFVGNDGPVLYFRTDLKAQKGRLIAIDLRNAGRENWKELIPESD
                     AKLQAVNYVGHRFVADYLRDAHSEIRLFDTGGKLVRTVELDGIGSASGFNGRSDDNET
                     FYNFASFATPPSVYRYDLASGEKQLLFRAKVAMKPEDYVVTQVFYTSKDGTKVPMFIS
                     HKKGLKLDGNNPALLYGYGGFDISMTPSFSISRLTWMNMGGVYAVANLRGGGEYGEAW
                     HEGGMKLHKQNVFDDFIAAGEYLVKNGYTKPAKLAIQGGSNGGLLIGAVENQRPDLFG
                     ATLPAVGVMDMLRFNQFTAGRYWVDDYGSSANADEFKALYAYSPYHNVKAGTKYPATL
                     ITTADTDDRVVPGHSFKYAAAIQEAQAGPAPVLIRIETRAGHGGGKPTAKVIEEVTDQ
                     LAFLVKNLSMKLPRGF"
BASE COUNT         1993 a         3241 c         3676 g         2079 t
ORIGIN      
        1 gaggtgccgc gctccaaagg gggcattgcc gcgcgcttcg aggacgtcga gagccgcggc
       61 gacctcgtcc caccgttgct ctctggcggc cgcaatcgag agcagcgacc atttcatctg
      121 tacgacggag acgcaggcga cgtggataat gacgagaaga tcgtcgcctt catccttggc
      181 gccgacgttc atgtcgatgt gacggcaccc tccggcgctc ggataaccgg ctggctgcat
      241 cgccgcggcg atcgtctcgt ggttcgggtc gaccggtacc caggccacgt agtcggcggg
      301 accgtcgtca tcgggcaggt actccgcgag cgtgcggagg gcgtcgtact tcaactgtgg
      361 cttctttaga gcggcgtcga tcatggcggc aagaaccgcg aaatgcggaa ggctcaactg
      421 attcggctcc gcagggccga cgttcatcag tgccaggaac gaccggattt cgtagcgcga
      481 catgccgaga tagacaccgg ggaccgccat cgccagaacg cgcgcgatcg ggtttctggc
      541 ccgtatcgcg agcgtccgtg cccgcgcgcc gagatcgggc gacggatcgg tatgcgcgga
      601 ctgccatagc agataaaacg cggaaatgag ctccttcgcc tgcgcgtcca tggaggggct
      661 cttctgcaag tctgttccgt cgcttccctc gtgagccggc aggccggaga ccttcgcgat
      721 gtcccctcct tcatggatgc cgatgtacac cgccgccgcg acgaaccgct gcacgtcggt
      781 atagttgcct tcggccagca ccttcggtag ctcgttggct atcagccgga gatggcgcca
      841 cagcacgagc tcgtcgccgg tcgcgcctga cgtgaactcc tgcgcaccct gctgcttcac
      901 gcgctcgttg gcgtcagtga ccgccttgaa ctgcggcgcg agatgcgcga ggtccagcat
      961 cgaccaagcg gcgtccgtga agcatttgga aacgtcgcgg tgaggaacga agaggcacgg
     1021 atacaccttc atgacgattt gagtgaagcc agccgtgtcc tgaaaccagt cctcggcatc
     1081 gatgcgctgg gcgagctgaa acgccacttt gtcgttgtcg tccctgcgaa gcccggagct
     1141 gacggctacc gccaccggcc gatatcctgc cgtgatcgcc ggcaggaagt catcagcgtt
     1201 cagaatgtac tgctgcggat cgcgaccgct ccgaatcgcg tagtcgagcg ccaccgaata
     1261 cagcatctcc accaccttct ccgccgcacc gggtgtgaag tcgatctcgg cgtagcgaag
     1321 gtactggtga ggaatctcgc tgacgcgcct ggtcagcgcc aggaggcgcg ggagcttcgt
     1381 cggagcggac ggcaggcgcg cgaaatcgtc cgagatcgcg gcgaagaggg cacggcgggc
     1441 ggcggacaat tcctggacgg agaccgcatc cgccttcaga agccattcca gccgctgcaa
     1501 cggaatgcgc ccttctccct cggacagcgc cttcacgacg gcgggctccg agctgtcgat
     1561 gggagcagcc gcttcgacca gcgatttcga cagcacttcc agagcagcgt ggtggcggcg
     1621 tgccgctgcg cggacgtcgg tgatcgatcg tttcgtggcc gcatcgaggc gcgtgatgac
     1681 cacgttcgtt tcgaagtcgc ggtccgacgc agaggtgtcg aacgcttccg caagcgcgat
     1741 gcgcgagagg tcgaagtcgt cccacaacgc gccgtgagct gtcgccacct gtcgcgcttc
     1801 gtccctcctg ccgatcacga tcagccggag gtatcgttcg cgcgcaaacc gtgcggcgtc
     1861 gcgcacctga ccttcccgca atgcggcctt cagcaaaacg tcgtacgcgc gcgccgcctg
     1921 cgccgcctcg gtgagtgtcg atgcaatcca ttctccgcat tcgatctgcg gcgacattgc
     1981 cgcggtaata cagtcgcggc atcgcggcgc atcgaggagc gccgccagct tgtcgaccac
     2041 ggacgcaggc ggcggagcgg ccgcttcttc cggatgttcg aagcgctcgg acagctcgca
     2101 tacggtgcgg ctggcgtcga cggagcatgc gttcgtcgcc ggctgctgcg tcgcggccgg
     2161 cagtcggcgc cactggaaaa tgagatcctt catacgcgcc gtgcgcgtcg ccaactcctc
     2221 cgccttcacc tcgcgaaagc cctcttcggc tttcgtcaga tcagccaagg ctggctcgaa
     2281 ggcccgcagc cgcgccaacg cgacgccgcg atagaaatga ccccaaggcg actcctcgat
     2341 ctttaggctc tgatttgccg cgtcgtatgc ggcgatttgg ccgtagtaga gccacgagag
     2401 aagatcattt tcgaccgggt ggtgaaagca gaacagatca tcgctctcca ccactgtacg
     2461 cgcctttttc aggagtgttg ccgcctcacc cgacggcgcc gccggcgcgc catcatcacg
     2521 tggcaggagc ggaaatttgg tgtcgacgac gccagaatcc gcgcgcaacg gtgcctcagc
     2581 cggcgcgccc gctgacggct tcctgaggac cgtcagcggc agcttttcgt tgtaattcgg
     2641 agagatgacc ggcgtctgct tcgacggaaa tgccgaggcc gtgcgctccg ccacctggcg
     2701 gcgaagaaac tcgtaaagct ctcccgtcgt gacgatgcca ttgccgtcgc caaacggctt
     2761 cccgtccgcc gcccctgcca gtccctccag caggtagtgc gtgaaaacgc cgtgtcgaag
     2821 cgccgggtct tcccacgcca cttcgcggct gccggccgat agaaaggcga cgaatgtcga
     2881 atcggtgccg agaagctgct ctttccagac ggatcggaat cgctcggtga tgttgccgtt
     2941 gtcgccgcgg cttccggcgt ccggaatcgc accgcccgca tgacaggcat cgatgaagat
     3001 gactacatgc cgcgctgaga tccacttgcg cagctcgtga atgaactcgt cgactcggta
     3061 tccgtacgcg gcgggcgccg tgttggaggc ctcgtacggc atcaggtatg cgtcgtcccg
     3121 ctccatctcg ccgtggccgg caaagaagat gaaaacgagg tcgtcgggcc gcgcggtgtc
     3181 gaggctctgg atggccttgt ggatctcgcc gcgggtcgct tcgccgccga ggagcttcgt
     3241 gacgtgcacc gacaagccgt ttgcggcggc ctggcgcgtc cagaagtcag cgaaggccgc
     3301 ggcatcacgg tcggtgtact ccaggcgatg atcggctgga tagttcggat atcccttcac
     3361 cccgatggcc aggacgtgga tgttgacctc gcccgcgaca gccgcagtcg cggcgaggac
     3421 ggaaaggaag gcgagcgcgg cggctttcat ttctgagacg ccgcgaagta gtagccgatc
     3481 gcgccggcga cgatccatga gaggaacgcg gtcgtgctca cgatgcgcgg gagggtctcc
     3541 tccatcgaca cgtgacctaa gagcggctgt aacagcagcg ccgccacgga ggcgatccac
     3601 agcgcagacc cgcacagcgc cgcggcccat gcggtcttcg gatccggtcc cgctttcttc
     3661 gccggtgcgg ccgccgtcca gaagaacgtc agcatggcgc ctaagtacgg gccgaatgcg
     3721 gcactgagcg tcgacagcgc gccggcgaac gactcaggcc cgatccagtc cgcgaggtag
     3781 agtgcataga tatagacgag aagagaagca aagccgccga accataggaa aagaagaacg
     3841 cgccgctgat ctcgaatggc catcactctg ccggcaggag cgcaacgagg cggtgttcgt
     3901 gctcgcgaaa aatgtcgtcg ctgctctccg gccgccaggt cgactcgagc ttgcgccgcg
     3961 tctggatagc agtcgaaatg gcgatcaatg cagtgccggg gactctccgc ggaaaggtaa
     4021 cgtcgacgag aaaagcggtt cggcggttgc ggatgttcgt ctcagacgtc tgccgcccgg
     4081 acggcagctc gcgcggattg gtgagaaatc gcagcctgcc gtcgggcagc aagtgttcat
     4141 agacggagaa gccgagagcc ccctgtagca cgtcatgcag tttttcggcg aaccgtgacg
     4201 cgttctgcac cgtccggcgc tgttcgttcg cggcgtggaa gtccgccagc gtcggccact
     4261 cttcgaacat gccgcgcgcg cctcgatacc gcgccttggc ggaggacgta cgaaccgctc
     4321 cgccgcaatc cgcaacgagc gtgtactcac catcgagcga ggcgtgaatc accgcttcgc
     4381 tgtcggccgc gcggactccc gccccggtga cgtaggcgct cgaacatccg ggtgcgctcc
     4441 acgtcagacg aacggtcccg gagtcgggga ccgcggttgc gtgaaagcgg aggtccgacg
     4501 caaacagtga gagcgtgacg atcgtcgccg ctgcagtact gtgcccgaga ctccttaatt
     4561 cgaacatgcg tggaacaatt ttaagtatat caggatcgtt ccggaccatg ccgattactc
     4621 aacgcgctct tcgaagagag cgcaggttca tacacctcgc caaaggctcg tttagagaag
     4681 gctttgataa cctgcggatc gcaatcgatg gtttccgtct ccgaagaaag agtcagcaat
     4741 cgacaattgg tcacgcgcgt tgatgtcaaa ggaaactcgg aggcgagaag caaatctacg
     4801 ttgagattcg ctattggtag cgcatacggg atttgaaccc gtgttaccgc cgtgagaggg
     4861 cggcgtcctg gacccctaga cgaatgcgcc agatggtggg agcgcgaggg tagccggggc
     4921 tgtctcgcgg tgtcaagcgc ggttaacctg atggtcggag ggagcattcc cgctgcgatg
     4981 cgcacggacc gcgtgggctg cgggcgcgat tgcatacgtc gacgctcctt ttcatgagcg
     5041 tcctggaatc gctgcccgga tctcgcctga gatgagcgcg gcgtgcgcgg taaggccgtg
     5101 atcgccctct tcgatagcga tgaatttcga ggagggggcc aggcttggct gccactggcg
     5161 ccaggtgttc tcgacgagat cgaaggggac gctttcgtcg ttgcggccca tgatcacggt
     5221 gacgggcgat ggcggaggat tgcggtcgac gcgcacctga aacatctgtt cgaagaactt
     5281 ccggtggatc ggaacctcgg cgttgcgggc gtgattgaag acgaggatcg ggtcgccgtc
     5341 cggaagtagt gtgcgccagc ggtcggcgat gccgagggct ggggcgatca ggaccatggg
     5401 ggcgatgatg ccgcgttggg caatggcgag ggcgacgagg gcgcccatcg agctgccggc
     5461 gatgagatcg ggaggatggg cgtgtgcgac attcatcgcg tgctcgacga tggcgttcca
     5521 gtcgagcgct tcgaacgagg ggacgttgag gtcgggtgtg atcagctcga tctccggctc
     5581 gagcatcggg cgaagcgcgg tgatcttcgc gctggcggga gacgatgcga agccgtgaaa
     5641 gtagaggagc gtggtcatcg aaagctaccg ggccattatc gatatggcgt tgcgagatcc
     5701 ctactcgatc ggcttcacgc ccatccgctt cttgatgtag agcggaatca gaatcagagc
     5761 cagcagcagg gcgcagacga tggccaggcg tttgagggcg tcgccctggg tcatggtgcc
     5821 gttgacgagg gagtctgcgc agtaggtgaa gacgaggtgg gacgggatga tgccgataag
     5881 cgtggcgagg gcgtagtcgc ggaagcgcat cttggcgacg cctgcggcgt agttccagac
     5941 ggcgaagggc gggccgggga tgaggcgcac gatgagcatc gacaggaagc ctgagctctc
     6001 gacctgcttg cgtactcctc ggccgacgct gccgaatttg tcgagcagac cctcgccgag
     6061 gaagccgccg acgtagtagg cggcgatggc gccgaggaag ccgccgatga tggcgtagag
     6121 cccaccgagc ttccagcccc agatcacgcc ggcggcgatg acgaagatgc tggcgggcag
     6181 cgcgaagacg cagccggcgg cgtagaggac gatgagcacg atcgggccgt accagagtcc
     6241 gcgcagccat gcgatgaagt cgttgatgtg cgctttagtg aggtaggcgc gcagcggtgt
     6301 gaagtagagc gcggcaacgc ctccggcgag gacgaggagc atcgcgagct tgatcgcgtt
     6361 tcgtttgttc attgggaggt tgggccgggg cgggagttta actcagagca gagacaagag
     6421 agcagagaca agagagcaga gacaagagag cagagacaag agacaagaga caagagacaa
     6481 gagacaagag acaagagaca agagacaaga gacaagagat cagatcaggt cgcgcctgtt
     6541 ctgatctctg atcttctgat ctcctgatct cttgtctctg atctcaggct tatggcgcag
     6601 aggtcaccgt ctccgtggcc tgcagtgcga tcgtaaccga gggcttggcc aggcctttgg
     6661 ggtgggagac accggagagg gtggtgaggg tgaggcggcg ccatttcact ggttgatcga
     6721 gcgtgatttc gatctttgag gtgactgcgg cgtcggcgag ggaatcgaat ttgatctcgc
     6781 ccatcgagtc gatggagcgg acgaaggaga gctcgatgat ggcgacgcgg tggccatcct
     6841 ggttttcgat cgcccgcaag gttcctcggc ctgggatggt cttcacgacg tgtccgagct
     6901 cgtcgtagca atcggcgagg agcgaggcgg gaacgttcca cgacgcgccg acggtaagcg
     6961 cgtacggcgc gatgcagaag ttggccgact cgacgcgaag ggcgcgctgc gccagctcgg
     7021 tgagccccat cgtctcgtcg acggtaaggt ccggtcctgt gtccggcgtg acggtgacgt
     7081 tcctcccctt catgacgaag agcagcgaac ggccgttcat gacgccgaag gtgttgcccg
     7141 attcctcttc gtggatcacg cggccgtaga cgcggcggaa gcgcgagccg gcggcgtcgc
     7201 gcgagagcgt ggtatcgctg tactcctcgc ggtcggcgct ggcgatgcgc ttcgtcgtca
     7261 gaacggtgct gtcgaggtcg cggatggtgc tggttccggc gatgtgcagc tccgcggtgc
     7321 cgtgaacgac gacaccggcg ggagcgttga tgacgggggc gatgcggacg gtttctacgg
     7381 cgcgggcgtt agcagcggcg attaaaaaca gcagcccaac gcagaacgcg gcggcgggac
     7441 gccgccgggc cagccggcgg gacgccagcg ttccaacgcg cattactgga tgctctccac
     7501 gatgatggcg atgccctggc cgccgccgat gcatgccgag gcgaggccga agcgcttctg
     7561 gcggcggcga agctcgtaga gcaacgtgat cacgagacgc gttcccgagg cggcgagtgg
     7621 gtgtccgagg gcgatggcgc cgccgttgac gttgagcttg gccgtgtcga tgccgagctc
     7681 tttcgatacg gccaggatct ggccggcgaa ggcttcgttg atctcgatca gatcgatgtc
     7741 gttcagcgac atccccgcct tcttcagtgc gatctttgtc gacggcacgg gaccgatgcc
     7801 catgatgtcg ggagcgcatc cggcgatgcc ccaggagacg atgcggccga tcggtttctt
     7861 gttcttcgcg ttgtcggcgc tcgtcacgac gaccatcgcg gcgccgtcga cgatgccgct
     7921 ggcgttgccg gcggtgacga agccgtcctt ggcaaaggca ggcttcagtt tggcgaggcc
     7981 ttccatcgtg gtgtcgggcc gcaggtgatc gtcggaggtg aacaactccc ctttgcggcc
     8041 gaccgccacg gcgacgatct cttctttcaa ccgtccagcg ctttccgcct cggcggcgcg
     8101 cttctggctc tgcagcgcaa actggtcctg ctcttcgcgc gagatgctgt atttgcgcgc
     8161 gaggttgtcg gaggtctggg ccatgtaaag gccgacgtag ctgtcgagca gggcaaccat
     8221 cagcgagtct tcgagcgcgc cctgtccgag cttcagccct ttgcgcgccc cgcggatcac
     8281 atgcggcgcc tgcgacatgt tctccatgcc cccggcgagt atggttgtcg cttcatcgag
     8341 ctggatctgc tcggcggcgc tgatgatcga ctggatgccg gagccgcaga gccggttgag
     8401 cgtgagcgcg ggcacttctt tggggacgcc cgctttgaga ccgacgtgac gcgcaccgta
     8461 gatggcgtcg cccgaagtct gcagtgcgtt gccgacgatc acatgattca cttcttcggg
     8521 tgcgacgccg ctgcgcgaaa gcgcttcttt cgcagcgacg acagcgaggt cgatggcgct
     8581 gatgtcactg aatgcgccgt tgtattcggc catcggcgtg cgagcgccgt cgaggatgac
     8641 gatgtctttc aatgtggact cctggttgat gagagatcag agactggaga ttcagagatc
     8701 agagactaga gattcagaga tcagagacta gagacagatc aggaagacga acagatcagc
     8761 ttcatgtcct gctctcttgt ctctgatctc cttctccttc ataaaaacag cgacttgaga
     8821 tcagcgatca gaaaagtcag agattgcggg ttcatgccct gatctcttgt ctctgatcgc
     8881 tgaccactgc ctcaaaaccc ccgcggcagc ttcatgctga ggttcttcac tagaaatgcg
     8941 agttgatcgg ttacttcttc gatgactttc gcggtcggct tgccgccgcc gtgtccggcg
     9001 cgggtctcga tgcggatgag taccggggcc gggccggcct gcgcttcctg gattgccgcc
     9061 gcgtacttga aactgtgtcc ggggacgacg cgatcgtcgg tgtcggcggt ggtgatcagc
     9121 gtggccggat acttcgttcc tgctttgacg ttgtggtacg gcgagtaggc gtagagcgct
     9181 ttgaactcgt cggcgttggc ggatgagccg taatcgtcga cccagtaacg gccagcggtg
     9241 aactggttga agcgaagcat gtccatcacg ccgacagccg gcaacgttgc gccgaagagg
     9301 tcggggcgct ggttctcgac cgcaccgatc agcaggccgc cattgctgcc gccctgaatg
     9361 gccagcttgg ccggcttcgt gtagccgttc ttgacgaggt actcgccggc ggcgatgaag
     9421 tcgtcgaaga cgttctgctt gtgcagcttc atcccgcctt cgtgccacgc ttctccgtac
     9481 tcgccaccgc cgcgcaggtt ggccacggca tagacgccgc ccatgttcat ccaggtaagg
     9541 cgcgagatgg agaagctcgg cgtcatggag atgtcgaaac cgccatatcc gtagagaagc
     9601 gccgggttgt tgccgtcgag tttgagtccc ttcttgtgcg agatgaacat cggcaccttc
     9661 gtgccgtcct tcgacgtgta gaagacctgt gtgacgacgt agtcctcagg cttcatcgcc
     9721 actttggcgc ggaagaggag ctgcttctcg ccgctggcaa ggtcgtagcg gtagacgctg
     9781 ggaggcgtgg cgaagctggc aaagttgtag aacgtctcgt tgtcgtcgga gcggccgttg
     9841 aatccgctgg cggatccaat gccgtcgagc tcgacggtgc gaacgagctt gccgccggta
     9901 tcgaagaggc ggatctcgct atgggcgtcg cggagatagt cggcgacgaa acggtgtccg
     9961 acgtaattga ctgcctgcag cttggcgtcg ctctcgggaa tgagctcttt ccagttctcg
    10021 cgcccggcat tgcgcagatc gatggcgatg aggcggccct tctgcgcctt cagatcggtg
    10081 cggaagtaga gcaccggccc atcgttgccg acgaatgaat agtcgttctc gatcttgtcg
    10141 acgaccgcca ccggcatcgc gtagggatcc tgaagatcct tgacgtggac ctggttcttt
    10201 gccgcggtgc cctgggtcgt ggtgatgatc aggtagcggc catcttccga gacctcggcg
    10261 ccatagttcc actccggctg cgaggcgtcg taccagacca cgacgtcgtc ggactgcggc
    10321 gtgccgaggc ggtggtagta aaccttctga tcgcggttca gactctggta ggccttccct
    10381 gcttcgacgg ccgggaagcg cgagtagaaa aatcccttat cgtccttcgt ccacgagatg
    10441 ccggaaaact tcacccactt gatctcgtcg gaaagcggct ggctggtggc gacatcggcc
    10501 acccggatga cttcccaatc cgatcccgcc tcggccgtcg catacgcggc gtacttggcg
    10561 tcatcgctga tggccagtcc gccgagggcg acggtgccat ccttcgacca ggtgttggga
    10621 tcgaggagaa cacgcggcgt gtcggtccac ttgtcgacgg tgtagacgac ggactgattc
    10681 tggaggccgt tgttcttgga gaagaagtag cggctgccac gcttgaaggg aaccgtgtac
    10741 ttctcgtagt tccagagcgt ggtgagctgc ttgcggatcg gttcgcgttc ggggatcgcg
    10801 ccgaggtagt cgaacgtgat cttgttctcg gcggcgaccc agtcggcgac atccttcgat
    10861 gtccgcacgt cgttttccag ccagcggtac gggtcagcga ctttgacgcc gtgataatcg
    10921 tcgacctgct cgaccttctt cgttttcgga tagtcgatcg gcgccgtgga tgcggcgaag
    10981 gcgatcagg
//
LOCUS       DATLVW010000212         7228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_256189, whole genome shotgun sequence.
ACCESSION   DATLVW010000212 DATLVW010000000
VERSION     DATLVW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256189"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VNN25_06825"
     CDS             complement(<1..999)
                     /locus_tag="VNN25_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HXH91276.1"
                     /translation="MTAPEAIAVVGLACRFPGASTPDQLWRNLRDGVESITFFTPEEL
                     QAAGVDAARMSDPRYVAAKGVIEEADGFDAGFFGFSPREAALMDPQQRVFLETAWSAL
                     EDAGYDAKNFPGPIGVFAGSILSVYLVRNLWPNRDAMATAGNFQIAVGNDPTFLATST
                     SYQLDLRGPSVSVGTACSTSLVAVHLACQSLLAFESDMALAGGVSIHLPLVGGYRYED
                     GGVLSPDGHCRPFDAAAQGTVSSDGAGVVVLRRLSDAVRDRDTILAVIRGSAINNDGR
                     MKVGYTAPSGDGESRVIAEALAMAGAEPESISLIETHGAATPLGDPIEVAALVDAFG"
     gene            complement(996..5117)
                     /locus_tag="VNN25_06830"
     CDS             complement(996..5117)
                     /locus_tag="VNN25_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXH91277.1"
                     /translation="MSEVRPKDTYPLSPMQEGMLFHTISAPDTDVYVQQLSCRLTGEL
                     RLADFERAWQTIMQRHPVLRTAFAWRGLPQPLQVVGEQVRVPLEVLDWRGDATDERLD
                     ALRASERLAAFDLRRAPLMRLKLVRLSESAHQLIWTWHHIILDAWSVPIVMEELFALY
                     AGLEEPEPSRPYRDFVAWQRSRDLAAAESFWRKELADVREPTPLGIEFPAPTNDGYGL
                     EFLPLPDEEVEALRNAARRLRLTLNTLVQGAWALLLSRYSRREEVVFGTAVAGRPPEL
                     AGVEKMVGLLVNTIAARIQVLPDKRLDEWLAELQHDQAESRRFEYAPLARVQGWSGVP
                     RGTQLFDSLLVFENTPLDVERLRGSGLELAGFDFVERASFPLTVMMEIRTHGKLGVGY
                     DRSRFDRASMLRLLSHMRTLLGQMCASPGLLLRDLDPMTDAEHVELIEWSREPFVSPA
                     TDASIVELFEAQVARTPDQTAAIFAAPGGDVSLTYRELDARANRVAQWLQTKGITRGD
                     RVAICMDASLERLASILGVLKAGAAYVPLEPSLPEARLQQMIADSGARLVLTEPPLIA
                     GDANGAITRPAAGDVAYIIYTSGSTGRPKGVPITHRSLRHLVDAQIESFQIGAGSRVL
                     QFASLSFDASVSEIFTALLAGARLYMAPRNLLLPSHEMLQLMELWEISVVTLPPSVLS
                     RLPESRLPSLRTLVSAGEACPADLAERWSRGRRFLNAYGPTEVTVCATIAEYRGGKPT
                     IGRPMGDARVYIVDEQLRPVPIGVAGEMLVGGPGVAQGYWNRPELSAVSFLDDPFAGG
                     RMYRTGDLARFLPNREIEFLGRRDDQVKVHGFRIEPGEIEAALRADPSVAQAAVAVDG
                     DHIVAYVVPSRSAQPEWWPSIAEYFVYDDLAYHAMTSDERRNESYRAAIRDHVRDKVV
                     VEVGTGPEAILSRFCAEAGARKVYAIELLPDTYAKACNRVRELGLDDRIEVILGDATK
                     VDLPEPADVCVSEIVGAIGGSEGAAMIMNGVRHMLREDAAMIPSRSTTMFAPVQLPES
                     LFDEMAFGELPARYVERIFAEVGHAFDLRLCVKGIGPSQLLAAPQVFEDLDFRGHTEP
                     EVRHQAGHVIERDGRIDGFLVWLTLDTGGGERIDILEHEHCWLPVFFPLHDSLDVRAG
                     DCIKSSCGAIVRGDAPHPDYFLEGMLSRGEETIPFRHESPRHGHAFRATPFYSRFFAG
                     GTVPRSTGRKDRLRRRLPDYMLPDVFVELDELPLLPSGKLDRRALPSAAPPKQRAEVV
                     PPRSETERAIASIWRDLLQIQDVGLQTNFFDQGGHSLLLLQVQDRVKRDLGFDVTVTD
                     LFNHPTVEALAGRLIRPAARPLDDQTKQRVAARQQALGRVNPRRAAATKEETQ"
     gene            complement(5144..>7228)
                     /locus_tag="VNN25_06835"
     CDS             complement(5144..>7228)
                     /locus_tag="VNN25_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXH91278.1"
                     /translation="PFADLLLEPMPLAQQDGQFDLNVEMKEDDRGQLVGAWKYAADLF
                     DAPAIERMAAHFETLLESIVAEPNCAIGALALLDAGDSREALARGAGEVAELPDKTIC
                     ELFEAQALRRPDAVAVTCGDDIVTYGELHDRVASLARSLVHRGARRDTIVAVLMSRGI
                     DFITALLAISKAGGAFLPLDARHASSRTAQIIEDSGAKLVIESIAHEPADDELPRVDG
                     HDLAYVMYTSGSTGKPKGVMVEHRGMVNHVLAKLSDLQIGEDGVLAQNGPPTFDIVVW
                     QCLGPLVVGGRVVVFPDDIAEDPSRLLDEVEARGVTVLQVVPSMLRAVLDDEPRDLTA
                     LRWIVPTGEALPTTLCRRWLARYPHIPLLNTYGSTECSDDQCHYVIRSLEQADEAVAI
                     ASIGTPIANMTAHVLDVNLRPVPAGVAGDLYIGGIGVGRGYVGDPERTAASFIPDPFS
                     AQPNARLYRTRDLARRRADGNLDFLGRVDAMIKVRGFRIEPGEIEAALMQHPAVAVAA
                     VVAREHPSRDRVLVAYAVLRAGTTAVPDELRHFLGERLPQYMVPTVITFLDALPLTSN
                     GKLDQKGLPSPQWDLTETQELVPPRTAAEKTIASIWSDVLGLERVGVTHDFFAVGGDS
                     IKSIQIVARCKRAGLEIRPSDLFQQSTIAALAALAERNSRQRNDTAHIPEFELSQEHL
                     DLALAQVEFDQA"
BASE COUNT         1212 a         2380 c         2465 g         1171 t
ORIGIN      
        1 gccgaacgcg tcgacgagcg cggcaacctc gatcggatcg cccagcggcg tggcggcacc
       61 gtgggtctcg atgagcgaga tcgactccgg ctccgcgccg gccatcgcca gcgcctccgc
      121 gataacgcgc gactcgccgt cgccgctcgg tgcggtgtag ccgaccttca ttcgcccatc
      181 gttgttgatc gccgagccgc ggatcacggc gagtatcgtg tcgcgatccc ggaccgcatc
      241 cgacagccgc cgcagcacga ccacaccggc gccgtcgctc gagacggtgc cttgcgccgc
      301 ggcatcgaac gggcggcagt ggccatcggg ggagagaacc ccgccgtctt cgtatcgata
      361 tccgccgacg agcggcagat ggatcgatac cccgccggcg agagccatat cgctctcaaa
      421 tgcgagtagg ctctgacagg cgagatgtac ggccacgagc gacgtcgagc aggcggtgcc
      481 gacgctcacg ctcggcccgc gcagatcgag ttgatacgac gtgctcgtgg cgaggaacgt
      541 cggatcgttg ccgacggcga tctggaaatt gccggccgtg gccatcgcgt cgcgattcgg
      601 ccagagattg cggaccaggt agacgctcag gatgctgccc gcgaagaccc caatcggccc
      661 cgggaagttc ttcgcgtcgt agcccgcgtc ttcgagcgcc gaccacgcgg tctccaggaa
      721 gacgcgctgc tgcggatcca tgagcgctgc ctctcgcggg ctgaagccaa agaagcctgc
      781 gtcgaagccg tccgcctcct cgatgacgcc tttcgcggcg acgtagcgcg ggtcgctcat
      841 acgggccgcg tcaacgccgg ccgcctgcag ttcctccggt gtaaagaagg tgatggattc
      901 gacgccatcg cggagattgc gccagagctg gtcaggcgtg ctcgctcccg gaaagcggca
      961 ggccaggccg acgacagcga tcgcttccgg cgccgtcatt gcgtctcctc tttcgttgcc
     1021 gccgcgcggc gaggattgac tctgccgagc gcctgctggc gcgcggcgac gcgttgcttc
     1081 gtctggtcgt cgagcggacg agcggcgggg cggatcaggc gtccggccag cgcttcgacg
     1141 gtcgggtgat tgaagaggtc cgtcaccgtg acgtcgaacc cgaggtcacg cttgacgcgg
     1201 tcctgcacct gcagcaggag tagagagtgc ccgccctgat cgaagaaatt cgtctgcagg
     1261 ccgacgtcct ggatctgcag gaggtcgcgc cagatgctgg cgatggcgcg ctcggtctcg
     1321 ctgcgcggtg gaacgacctc ggctcgctgc ttcggcggcg cggcggatgg cagagcgcgg
     1381 cgatcgagct ttccgctcgg cagcagcggc agctcgtcga gctcgacgaa gacgtcgggc
     1441 agcatgtagt caggcagccg ccggcgaaga cgatcctttc ttccggtgga gcgggggacc
     1501 gtgcctccgg cgaagaagcg gctgtaaaac ggagtggcgc ggaacgcgtg gccgtgccgc
     1561 ggcgactcgt ggcggaaggg aatcgtctcc tctccgcgcg acagcattcc ttcgagaaag
     1621 tagtcgggat gcggagcgtc gcctcgcacg atcgcgccgc acgacgactt gatgcaatcg
     1681 cccgcgcgca cgtcgagcga gtcatgcagc gggaagaaca ccgggagcca gcagtgctcg
     1741 tgctcgagga tgtcgatgcg ctcgccgccg ccggtgtcga gcgtgagcca gaccaggaat
     1801 ccatcgatcc gtccatcgcg ctcgatcacg tgcccggcct gatggcgaac ttccggctcg
     1861 gtgtgcccgc ggaagtcgag atcctcgaat acctgcggcg cggcgagcag ttgcgacggc
     1921 ccgatgccct tcacgcagag gcgcagatcg aacgcgtgac cgacctcggc gaagatgcgc
     1981 tcgacgtagc gcgccggcaa ttcgccgaac gccatctcgt cgaacagcga ttcaggcaac
     2041 tgcaccggcg caaacatcgt ggtgctccgc gacggaatca tcgcggcgtc ttcgcggagc
     2101 atgtgccgca cgccgttcat gatcatcgcg gcgccttcgc tgccgccgat cgcgcccacg
     2161 atttcggaca cgcagacgtc cgcgggctcc ggcagatcga ccttggtggc atcgccgagg
     2221 atcacttcga tgcggtcgtc gagtccaagc tcgcgcacgc ggttgcacgc tttggcgtag
     2281 gtgtcaggca gcaactcgat cgcgtagacc ttgcgcgccc cagcctcggc gcagaaccgc
     2341 gacaggatgg cttccggacc ggtgccgact tcgaccacga ctttgtcgcg cacgtggtcg
     2401 cggatcgccg ctcgataact ctcgttacgt cgctcgtcgc tcgtcatcgc gtgatacgca
     2461 aggtcgtcat agacgaagta ctcggcgatc gacggccacc actccggctg cgccgatcga
     2521 ctcggtacga catacgcgac gatgtggtca ccgtcgacag cgacggccgc ctgtgcgact
     2581 gaggggtcag cgcgcagcgc ggcctcgatc tcgccgggct cgatgcggaa gccgtggact
     2641 ttgacctgat cgtcgcggcg tccgaggaac tcgatctcgc ggttcggcag aaagcgggcg
     2701 agatcgccgg tgcgatacat cctgccgcca gcgaacggat catccaggaa gctcacggcg
     2761 ctcaactcgg ggcgattcca gtacccctgc gccacgcccg gtccgccgac gagcatttct
     2821 cccgcgacac cgatcggaac ggggcgaagt tgctcatcga cgatgtagac gcgcgcgtct
     2881 cccatcgggc ggccgatggt cggcttgccg ccgcggtact cagcgatggt tgcgcagacc
     2941 gtgacctcgg tcgggccgta cgcattgagg aagcgtcgtc cgcgcgacca gcgctcggcg
     3001 agatcggccg gacacgcctc gccggcggag acgagcgtcc gcagcgacgg caggcgcgac
     3061 tcggggagcc gcgagagcac cgacggcggt agcgtgacga cgctgatctc ccagagctcc
     3121 atcaactgca gcatctcgtg cgacgggagg agcaagttgc gcggcgccat gtaaagcctc
     3181 gcgcctgcga gcaatgcggt gaagatctcc gatacggacg catcgaagct gagcgatgcg
     3241 aactgcagaa cgcggctgcc ggctccgatc tggaacgact cgatctgcgc gtcgacgagg
     3301 tgccgcagcg agcgatgggt gatcggaacg cccttcggcc ggccggtcga tcctgacgta
     3361 tagataatgt aggcgacgtc gccggcggcc ggccgcgtga tcgcaccatt cgcatctccg
     3421 gcgatgagtg gcggctcggt gagcacgagc cgcgcgccgc tgtcggcgat catttgctgc
     3481 aggcgtgcct cggggaggga aggctcgagc ggaacgtacg ccgcgccggc cttgagcacg
     3541 ccgaggatcg acgcgagacg ctccagcgac gcatccatgc agatcgcgac gcggtcgccg
     3601 cgcgtgatgc ctttcgtctg cagccactgc gcgacgcggt tcgcgcgcgc atcgagctcg
     3661 cggtacgtaa gcgagacgtc gccgccgggg gcggcgaaga tcgccgcggt ctgatccggt
     3721 gtccgcgcga cctgtgcctc gaagagctca acgatcgagg catcggttgc cggtgatacg
     3781 aacggctcgc gactccactc gatcagttct acgtgctcgg catccgtcat cgggtcgagg
     3841 tcgcggagga gcaagcccgg gctcgcgcac atctgcccga ggagcgtgcg catgtgcgac
     3901 agcaggcgaa gcatcgacgc gcgatcgaaa cggctgcggt cataaccgac gccgagcttg
     3961 ccgtgcgtgc ggatctccat catcacggtc agcgggaaac tggcccgctc gacgaaatcg
     4021 aagccggcga gctcgaggcc gctgccgcgc aggcgctcga cgtcgagagg ggtgttttcg
     4081 aaaacgagca ggctgtcgaa caactgcgtg ccgcgcggaa cgccgctcca tccctgcacg
     4141 cgcgccaacg gcgcatattc gaagcggcgc gactccgcct ggtcgtgctg cagttcggcg
     4201 agccattcat cgaggcgttt atccggcagc acctgaatgc gagccgcgat cgtgttcacg
     4261 agcagcccga ccattttctc gacaccggcg agctccggcg ggcggccggc gacggcggtg
     4321 ccaaagacca cttcctcgcg ccggctgtag cggctgagca gcagcgccca agcgccttgc
     4381 acgagcgtgt tcaacgtcag ccgcaggcgg cgtgcggcat tccgcaatgc ttcgacttcc
     4441 tcgtccggca gcggcaggaa ctcgaggccg tagccgtcgt ttgtcggagc cggaaactca
     4501 attccgagcg gcgtcggctc gcggacatcc gcaagctctt ttcgccagaa actttccgcc
     4561 gccgcaagat cgcgtgagcg ctgccaggcc acgaagtcgc ggtacggccg gctcggctcc
     4621 ggctcttcca gtcccgcgta cagcgcgaaa agctcctcca tcacgatcgg caccgaccag
     4681 gcgtcgagga tgatgtggtg ccacgtccag atcagttgat gcgcgctctc cgacaaacgc
     4741 acgagcttga gccgcatcag cggcgcgcgg cgaagatcga aggcagcgag ccgctcgctc
     4801 gcgcgcaatg cgtccaaccg ttcgtctgtg gcgtcgccgc gccagtcgag gacctccagc
     4861 ggcacgcgca cctgctcgcc gacgacctgc agcggctgcg gcaggccgcg ccacgcgaac
     4921 gcggtccgca ggacggggtg ccgttgcatg atggtctgcc acgcgcgctc gaaatcagcg
     4981 aggcgcagct cgccggtgag gcgacacgag agctgctgca catacacgtc ggtgtcgggc
     5041 gccgaaatgg tgtgaaagag cattccctcc tgcatcgggg aaagcggata ggtgtcttta
     5101 gggcgtactt cgctcatggt ttggtggtct gtggtggttt gccctaggcc tggtcgaact
     5161 cgacctgcgc gagagcgagg tcgaggtgtt cctggctgag ctcgaattcg gggatgtgcg
     5221 cggtgtcgtt cctttgccgc gagttgcgct ccgccagcgc ggcgagcgcg gcgatcgtcg
     5281 actgctggaa cagatcgctg ggacggatct ccagtcccgc gcgtttgcat cgggcgacga
     5341 tctgaatgct tttgatcgaa tcgccgccga ccgcgaagaa gtcgtgcgtg acgccgacgc
     5401 gctcgaggcc gagaacgtcc gaccagatcg acgcgatcgt cttctccgcc gcggtccgcg
     5461 gtggcacgag ctcctgcgtc tccgtcaggt cccactgtgg cgacggcagg cctttctgat
     5521 cgagcttgcc gttcgaggtc agaggaagtg cgtcgagaaa cgtgatcacg gtcggcacca
     5581 tgtattgagg aaggcgctcg ccgaggaagt ggcgcagctc gtcgggaacc gccgtggtcc
     5641 cggcgcgcag aacggcgtag gcgacgagca cgcggtctcg cgacgggtgc tcgcgcgcga
     5701 cgaccgcggc caccgcaacg gcgggatgct gcatgagcgc cgcctcgatc tcgccgggct
     5761 cgatgcggaa gccgcgcact ttgatcatcg cgtcgacgcg cccgaggaag tcgagattgc
     5821 cgtcggcgcg acggcgcgcc agatcgcgcg ttcgataaag acgcgcattc ggctgtgcgc
     5881 tgaatggatc ggggatgaat gatgccgcgg tgcgctcggg atcgccgacg tagccgcggc
     5941 cgacgccgat gccgccgatg tagagatcgc ccgcgacgcc ggcaggtacg ggacggagat
     6001 tcacgtcgag cacatgcgcg gtcatgttgg cgatcggtgt accgatcgag gcgatggcca
     6061 ccgcctcgtc cgcctgctcg aggctgcgga tcacgtagtg gcattgatcg tccgagcatt
     6121 cggtcgagcc gtaggtgttg aggaggggaa tgtgcggata gcgcgcaagc cagcgccggc
     6181 aaagcgtcgt cggcagcgct tcgccggtcg gcacgatcca gcgcaatgcg gtgaggtcgc
     6241 gcggctcgtc gtcgagcacc gcgcgcagca tcgacggcac cacctgcagc accgtcacgc
     6301 cccgcgcttc cacctcgtcg agcagtctcg aaggatcttc cgcgatgtca tcgggaaaga
     6361 ccaccacgcg cccgcccact acgagcgggc cgagacattg ccagaccacg atgtcgaagg
     6421 tcgggggacc gttctgcgca agcacgccgt cctcgccgat ctgcaagtcc gacagcttcg
     6481 ccagcacgtg gttgaccatt cctcggtgct cgaccatgac gcctttcggc ttgccggtcg
     6541 agccggatgt gtacatgaca tacgcgagat catgcccgtc gacgcgcggc agttcgtcgt
     6601 ccgccggctc gtgcgcgatg ctctcgatca cgagcttcgc gccgctgtct tcgatgatct
     6661 gcgccgttcg cgacgaggcg tgacgcgcgt cgagcgggag gaacgcaccg ccggctttcg
     6721 agatcgcgag gagggcggtg atgaagtcga ttccgcgcga catgagcaca gcgacgatgg
     6781 tgtcccggcg cgcgccgcga tggacgagac tgcgcgccag actggcgacc cggtcgtgca
     6841 gttcgccgta cgtcacgatg tcgtcgccgc aggtgacggc gactgcatcg gggcgccgca
     6901 gcgcctgcgc ttcgaagagc tcgcagatcg ttttgtcggg cagttccgcc acctctcccg
     6961 caccgcgcgc gagcgcctcg cggctgtcgc cggcgtcgag caacgccagc gctccgattg
     7021 cgcaattcgg ttcggccacg atcgactcga gcagcgtctc gaagtgcgcg gccatccgct
     7081 cgatcgccgg cgcgtcgaac agatcggccg cgtatttcca cgcgccgacg agttgcccgc
     7141 gatcgtcctc tttcatctcc acgttgagat cgaactgccc atcctgctgc gcgagcggca
     7201 tcggctcgag cagaagatcg gcgaacgg
//
LOCUS       DATLVW010000213         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_586900, whole genome shotgun sequence.
ACCESSION   DATLVW010000213 DATLVW010000000
VERSION     DATLVW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586900"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VNN25_06840"
     CDS             complement(<1..1671)
                     /locus_tag="VNN25_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91279.1"
                     /translation="MRRRHALLLAALIALALVTWLRIHLIDTLPDQGYFAKYLAFTDR
                     ILAGRIPRDRLADLSPGYLWFVVLLRSLGAGMMAIRTLQIVMVSIAAICCGLAARRFG
                     TIAMIAAPVFLLASRAALVCATEAEPETLILLLVSAGLAALSYSHSASGALFGLAAAC
                     RPVVMLGAAAIAIVERSWKLIVAAIVPLVLLLIVNIALTGEVVLMDPGTVFYEGMNPS
                     ATGYEGVQPRIVNDIERTSIEPDYLHVAYRIVASRSAGRPLTRAESNRFWTGKALAFV
                     RAYPRAALMLTLHKVFFAIHSHDAYDLATMARKDALLGRYPFLPFGAVLALAGAGVLL
                     ARERRRELLPFLVFALAGFAVLVAFYVTARQRNAILPALAVLAAVGVSEVIARRHLIA
                     AVAVIIVAILLGINGPAQIEDTNGWLGVRNAFDDALTLEQQGRWADADRLLAQLEADD
                     YHPIRENRAVPSVAFYRARAALHLGHDPRPLLDRATSEAPGNEHVLAMRARLGDGEAG
                     KLLFALHDAMTARRALSGGGEWELAVAGLASKSSTTPSPTPPPHIPPLP"
     gene            complement(1682..2728)
                     /locus_tag="VNN25_06845"
     CDS             complement(1682..2728)
                     /locus_tag="VNN25_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91280.1"
                     /translation="MREHYNNQMQAYGSDRVRPGEGEQFVLMARFPKQWTPRAAKTLT
                     STEFPGTAVLWEERYFEVVDAEGLPQGGVKYVLEPWREMHIMRTTDRYDEASEAERFV
                     EWRAGFSRERKRKSANALALLTGHLPAAVQEELGRNLGVLPRRLTLISVAGVYAVVIG
                     LMLCVVGGVIEQNSRPLLFYILTGYLFIESSFRFLVSWLGGRPMGSSLGLIAYLVYYF
                     TVADRTRAVSPFAEEKGMSIAFSATPEDRVASDALVMRESLVTLLNPADQARVAERFG
                     YDYKRQSRFIAAVILVFASLGIATSIHNRAVISFLLATILAGEQLYRLTVLGRRPAGS
                     VLGILARPFVRKLL"
     gene            2772..3158
                     /locus_tag="VNN25_06850"
     CDS             2772..3158
                     /locus_tag="VNN25_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91281.1"
                     /translation="MSDKPAKDCAVLVVEDDPAIQRLVKKVLGREGYQVETACDGIEA
                     VLKLGILDYDVIILDLMMPNLDGFVFVSTMAEHDPERLKRVIVTSAASPGVIKARMKG
                     EPFLVLPKPFDIQELIAHVRACVKAQ"
     gene            complement(3246..3680)
                     /locus_tag="VNN25_06855"
     CDS             complement(3246..3680)
                     /locus_tag="VNN25_06855"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HXH91282.1"
                     /translation="MPDIHASFADYASHHQTKGNKWFHRFGIPMIMLTSIGMLARVGL
                     GAVAGVRIDAGMLLIALAAIYYFTLEWRLAIPMLIVSIGFYLIGRWLPMSVNVVLFIL
                     GWIFQFIGHAVYEHRQPAFFRNVFHLLIGPLWILNDVIPVVK"
     gene            complement(3710..>4246)
                     /locus_tag="VNN25_06860"
     CDS             complement(3710..>4246)
                     /locus_tag="VNN25_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91283.1"
                     /translation="GIGGNYILHEYQERSKEKELELDDHKELVKRQEESAINAFQLLG
                     EAHYSAIALNKLAQSAPPPGLTQAQIDLHQKNNELIWSRSSDFLERWEKEKTPTGMRL
                     FYFYEGDRVLTAWVRVKEAGDQMLKAALNRVLMRMASPGAPLPRTSPQGEAQEEQKFD
                     DAVNDLCRVLEQAHRANK"
BASE COUNT          871 a         1302 c         1366 g          707 t
ORIGIN      
        1 cggaaggggt gggatgtggg gagggggggt gggagatgga gtagtcgatg atttgctggc
       61 caacccggca accgccagct cccactcccc accccccgac aacgcccgcc gcgccgtcat
      121 cgcatcgtgc agtgcgaaca gcagcttgcc cgcttcgcca tccccgagcc gcgcccgcat
      181 cgccagcaca tgctcgttcc ccggcgcctc ggacgtcgca cgatcgagca gcgggcgcgg
      241 atcgtggccg aggtggaggg cggcgcgggc gcggtagaag gcgaccgagg ggacggcgcg
      301 attttcgcgg atcgggtgat agtcgtcggc ctcgagctgc gcgagaagtc gatcggcgtc
      361 agcccaccgg ccctgttgtt cgagcgtgag cgcatcgtcg aacgcgttcc ggacaccgag
      421 ccagccgttc gtgtcttcga tctgggccgg accgttgatg ccgaggagga tggcgacgat
      481 gatgacggca acggcggcga tcaggtgccg tcgcgcgatg acctcggaga cgccgactgc
      541 cgcgagcaca gcaagcgcag gaaggatggc gtttcgctgc cgagcggtca cgtaaaacgc
      601 gacgaggacc gcgaaaccgg cgagcgcgaa gacgaggaac ggaagcagct cgcggcggcg
      661 ctcgcgcgcg aggagcaccc ccgcgccggc gagagcgagc acggcgccga acgggaggaa
      721 cggatagcgg ccgagcagcg cgtccttgcg cgccatcgtg gccaggtcat acgcgtcgtg
      781 actatgaatc gcgaagaaca ctttgtgcag cgtgagcatg agcgcggcac gcggatacgc
      841 gcgcacgaac gccagcgcct ttcccgtcca gaagcgattc gactccgcgc gcgtcagcgg
      901 ccggccggcg gagcgcgagg ccacgatgcg ataggcgacg tgcaggtaat cgggctcaat
      961 cgatgttcgc tcgatgtcgt tcacgattcg cggctgcacg ccttcgtagc cggtcgcgct
     1021 cgggttcatc ccttcgtaga aaaccgttcc cggatccatc aggacgactt cgccggtcaa
     1081 cgcgatgttg acgatcagca ggagcacaag cgggacgatt gccgcgacga tcaacttcca
     1141 cgatcgctcc acgatcgcga tcgccgcggc accgagcatg acgaccggcc ggcaggcagc
     1201 ggccagtccg aagagcgctc ccgaagctga gtgcgaatac gaaagcgccg cgagaccggc
     1261 gctaaccagg agaaggatca acgtctccgg ctccgcttcc gtcgcgcaaa cgagcgcggc
     1321 acggctggct aatagaaaca ccggcgcggc gatcatggcg atcgttccga aacggcgcgc
     1381 ggcgagtccg cagcagattg cggcgatgct caccatgacg atctgcagcg ttcgaatcgc
     1441 catcatgccg gcgccgagag agcgcagcaa cacaacgaac cagagatagc cggggctgag
     1501 atcggccaga cgatcgcgcg gaatcctgcc ggccagaatg cggtcggtga acgccaggta
     1561 cttcgcgaaa tagccctgat cgggaagcgt atcgatgagg tggatgcgca gccaggtgac
     1621 caacgccaac gcgatgaggg cggcgagcag caacgcgtga cggcggcgca tcgacgcgaa
     1681 tctacagcag cttgcgcaca aacggccgcg ccagaatgcc caggacgctt ccggccgggc
     1741 gccgcccgag gacggtcagc cgataaagct gctcgccggc gaggatcgtt gcgaggagaa
     1801 aagagatgac ggcgcggttg tgaatggatg tggcgatgcc gagcgatgcg aagacgagga
     1861 tcaccgcggc gatgaatcgg gactggcgct tgtagtcgta gccgaagcgc tctgccacgc
     1921 gcgcctgatc cgcgggattg agcagcgtca cgagcgattc gcgcatgacc agcgcatcgc
     1981 tggcaacgcg atcctccggc gtcgcgctga aggcgatcga cattcctttc tcctcagcaa
     2041 atggcgagac cgcccgcgta cgatcggcga ccgtgaagta ataaacgaga taggcgatca
     2101 gaccgagcga ggagcccatc ggccgtccgc cgagccagct cacgaggaat cgaaaggacg
     2161 attcgatgaa gaggtaccca gtcagaatgt agaagaggag cggccgcgaa ttctgctcga
     2221 tcacgccgcc cacgacgcag agcatcagcc cgatgacgac cgcgtagacg ccagcgacgg
     2281 agatgagcgt cagcctcctg ggcaacacac cgagattgcg ccccaactct tcctgaacgg
     2341 cggccggcag atgtccggtc agcaatgcca gcgcgtttgc cgatttccgt ttgcgctcgc
     2401 gcgaaaatcc ggcgcgccat tcgacgaacc gttccgcttc gctcgcttcg tcgtagcggt
     2461 cggtcgtacg catgatgtgc atctcgcgcc acggctcgag cacgtatttc accccaccct
     2521 gcggcaatcc ctcggcatcc acaacctcga agtagcgctc ctcccaaagc accgccgtcc
     2581 ccggaaactc ggtgctggtc agcgtcttcg cggcgcgcgg cgtccactgc ttcggaaagc
     2641 gcgccatcag gacgaactgc tcgccttcgc ccgggcggac gcggtcggag ccgtatgcct
     2701 gcatctggtt attgtagtgc tcgcgcaaag gcgggagcgc ttcttgctgc cgttgaggct
     2761 ggaaaccaaa ggtgagtgat aagccagcga aggactgcgc cgttctcgtc gttgaggacg
     2821 atccggcgat tcagcgcctc gtcaagaagg tgctcggccg cgaggggtat caagtcgaga
     2881 ccgcctgcga cggcatcgaa gccgtgctca agctcggaat cctcgactac gacgtgatca
     2941 ttctcgatct catgatgccc aacctcgacg gattcgtctt cgtaagcacg atggcggaac
     3001 acgatcccga gcggctcaag cgtgtcatcg tgacgagcgc cgcatcgccc ggcgtgatca
     3061 aggcacgaat gaagggagag ccgttcctcg tgctgccgaa accgttcgac atccaggagc
     3121 tgattgctca cgttcgggcg tgcgtgaagg cgcagtgaag cagagatcag cgacaaagag
     3181 atcagagatc aggtggcggg gtgcgtctgc tttttgtctc tgatctcttg tctctgatct
     3241 ctgcgctact tcacgaccgg aatcacatca ttcagaatcc acagcggtcc gatcagcagg
     3301 tgaaagacgt tacggaaaaa cgccggctgc cggtgctcgt agaccgcgtg tccgatgaat
     3361 tgaaaaatcc atccgaggat gaagagaacg acgttgacgg acatcggcag ccatcggcca
     3421 ataaggtaga acccgatgga aacgatgagc atcgggatcg ccagccgcca ctccagcgtg
     3481 aagtagtaaa tggcggccag ggcgatgagc agcatccctg catcgatgcg cacaccggcc
     3541 acggcgccga ggccgacacg ggccagcatg ccgatggacg tgagcatgat catcgggatc
     3601 ccgaagcggt gaaaccactt gttccctttc gtctgatgat gcgaggcgta gtcggcgaag
     3661 gaagcgtgaa tgtccggcat ggggaaagtg taacgctgtc tgactgcact cacttgtttg
     3721 cccggtgtgc ctgctcgagc acgcggcaga gatcattcac agcgtcgtcg aacttctgtt
     3781 cttcttgtgc ctcaccttgc ggcgaggtcc gcgggagtgg agcgcccgga ctggccatgc
     3841 gcatcaggac gcggttcagg gctgccttca gcatctgatc gcccgcctcc ttcacgcgta
     3901 cccacgccgt gaggactcgg tcaccttcat agaaatagaa aaggcgcatg ccggtgggag
     3961 ttttctcctt ttcccaccgt tccagaaagt cagaggagcg gctccagatc aactcattgt
     4021 tcttttgatg caggtcgatc tgcgcctgcg tcagaccagg cggaggcgcc gactgggcga
     4081 gcttgttcag agcaattgca gagtaatgcg cctctccgag gagctgaaaa gcattgatgg
     4141 cactctcctc ctgccgcttg accagttcct tgtgatcgtc gagctccagc tccttttcct
     4201 tggaacgctc ctgatactca tgaagaatgt agtttcctcc gatccc
//
LOCUS       DATLVW010000214         6403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_177100, whole genome shotgun sequence.
ACCESSION   DATLVW010000214 DATLVW010000000
VERSION     DATLVW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177100"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1064
                     /locus_tag="VNN25_06865"
     CDS             108..1064
                     /locus_tag="VNN25_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH91284.1"
                     /translation="MSVLDILAKPLAHFGIPTGKLGNVVFLPGILGTDLADDENRELW
                     IFPPRLKELLLSSDGKSEDNSKRVVHPTAPNFPHAAMLHVLNLSWNVLSFGYDWRKDI
                     RESALKLDVAVRQRFGNEAFHIVAHSMGGLVARVLRANSPDIQRGGKLVMLCTPNHGS
                     YLALHALTLGVAAQALFGLFRAPVAPADALAAAQTWPGLYQLVPSPVKDPAIQGFYSA
                     PPPGLSRAHVDDARALHEFLAKVPSHDDFVYIGGANLPTVDGMKFLPTTTDGDGVVSH
                     RLGFLSGVRTFTLPGGHVDLLSNPFVLASISPLLHGDIQGLL"
     gene            1192..2325
                     /locus_tag="VNN25_06870"
     CDS             1192..2325
                     /locus_tag="VNN25_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH91285.1"
                     /translation="MPRVFVSHSSRDKSFVRPLVAEMRRYGLDVWFDESDLHVGDVID
                     PALCEGIDASDFVVVVLSPASVDSPWVAAEWRYGCGKEAECGKPTVVPILLERCTMPP
                     PLKAKKYADFTGNRSHAFVSLLEAIRPLELVHRSYTVLKGRTVDIGRRGHSVTEGPQR
                     IMTLIAGGDEWAAVALLETVGPRDAAIRASAFALGGINAYFESLDGELDPEYMVRHMA
                     ITADTAIRYVRTHEALEGHALGGSCAVAVQTRGILHTAALGRAGALCHWSSSSGSCFL
                     PVQSDFTAKLVTGAGDPGFALTHPLGYLADGRLEFGVHPTEFRTAGDFVLLLSRPLPD
                     NEQARAELCSALDGRADGHVLVAHVCNNQAPPTDDFMVLALRR"
     gene            complement(2793..3320)
                     /locus_tag="VNN25_06875"
     CDS             complement(2793..3320)
                     /locus_tag="VNN25_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91286.1"
                     /translation="MSTTVNNTAVPSNPDPAKLTPEAVVAQLRAIGSQIDDLAPLSKE
                     QRALLKKRLRRQPKTIVEASINVIGVLDNVSQAIGEPLDEVRQLQDESLRWDAAVEEA
                     RAFLNGLEGAALIRRQRLGLLAMQAYTIGSQLARDPARAVLVPHVEEVKRLKGVARRK
                     KSQTPPPAPPAPESE"
     gene            complement(4773..5873)
                     /locus_tag="VNN25_06880"
     CDS             complement(4773..5873)
                     /locus_tag="VNN25_06880"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXH91287.1"
                     /translation="MKKSTKSVSNKTMKNGVAAVGLDLSDRTGRFHGIDDEGKTIETG
                     TVTLRAVELQRWAGAIAPTVIAIEAGTHSPWISRLLTACGHEVIVANPVKVALITRND
                     RKSDVIDAEYLARLARFDRELLHPIRHRGEQAQIDLQIIRTRVVLVESRTKLISHVRG
                     AIKSFGERLPACGTEAFVNKTKALVPAALKEALSPVYTEIDQLTKTVTAYDRQVLRMV
                     NERYPEVERITQIKGVGALTGLAFVLVIEDAARFRRSRSVGAYLGLTSKRDQSGDDDP
                     QRGITKAGDRLMRRLLIQSAHYIQGPFGDDCDLRRHGIRIAERGGKRAKRRATIAVAR
                     KLSVLMHRLWVSQETYEPLRNSSKGIGLSVAA"
     gene            6007..>6403
                     /locus_tag="VNN25_06885"
     CDS             6007..>6403
                     /locus_tag="VNN25_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine acetyltransferase"
                     /protein_id="HXH91288.1"
                     /translation="MKRLLPLFFFCASVAFAGDGTLQMATLGDLKLDSGQVIHDCRVG
                     YRTYGTLKADRSNVIVVTTWFGGTTAGLTGWIGPGKLYDSSKYYIIAIDALGDGVSSS
                     PSNSGAQHGADFPPISMRDLVRSQHQLLTH"
BASE COUNT         1089 a         2009 c         1891 g         1414 t
ORIGIN      
        1 tccgaggctg tgacgacgag tgaggcgcgc cgcggcccag caaagagatg aggcaaattc
       61 agggtatgga tttcttgccg acggaactct cctagaaggg gtgttcgatg tcagtgctcg
      121 acatacttgc gaaacctttg gctcatttcg ggattccgac tgggaagctg gggaacgttg
      181 tgtttctgcc aggaatacta gggactgacc tcgcggatga cgagaaccgt gagctctgga
      241 tctttccgcc gcgcttgaaa gagctgctgc tttcgtcgga cggcaagagt gaagacaact
      301 cgaagcgcgt agtccatcca actgcgccaa actttcccca tgcagccatg ttgcacgttc
      361 tcaatctgtc gtggaatgta ctttcgttcg gctacgattg gcgcaaagat attcgtgaat
      421 cagcgctgaa gcttgacgtg gctgtgcggc agcgcttcgg gaacgaggcc ttccatatcg
      481 tcgcgcattc catgggagga ttggtcgcgc gagtccttcg cgcaaactca ccggacatcc
      541 agcgtggcgg taagctcgtc atgctgtgca cgcctaacca cgggagctac cttgcactcc
      601 atgcgctgac gcttggtgta gctgcgcaag cactttttgg tttgttccgc gcaccggttg
      661 cgccggctga cgccttggca gcggcgcaaa cgtggccggg cttgtaccaa ctcgttccct
      721 ctcccgtgaa agatcccgcg atccaaggtt tctactccgc gccaccgccc ggtctatcgc
      781 gggcccatgt tgacgacgcg cgggcgctgc acgagttcct ggccaaagtc ccgagtcacg
      841 atgacttcgt ctacatcggc ggtgcaaacc tccccacggt tgatggaatg aaattcctcc
      901 ccacgacaac tgatggagac ggtgtggtct cccatcgtct aggcttcctg tccggcgtgc
      961 gaacgtttac cctgcccgga ggtcatgtcg atctactatc caatccgttc gtgttggcct
     1021 cgatttcgcc acttctgcac ggagacattc aggggttact ctgatctgca aacacggctt
     1081 tctaacggta cacttccgca tgcggttggc gcggcagaac accaagccgt caggtggctc
     1141 cgcgatgtga ggagccacga ggccaacatc tggcacgcta actagtcgac aatgcctcgc
     1201 gtgtttgtgt cgcactcatc cagggacaag tcttttgtcc gcccgctagt cgctgaaatg
     1261 aggcgctacg gtctagatgt gtggtttgat gaatcggact tgcacgttgg tgatgtcatc
     1321 gaccctgcgc tctgcgaagg aatagacgcg agtgactttg tggtcgtcgt gctctccccc
     1381 gcttcggtag actcgccatg ggttgcagcg gaatggcgat atggctgcgg caaggaggct
     1441 gaatgcggga agccgacggt tgtccccata ctactcgagc gttgcacgat gcctccaccg
     1501 ctcaaagcca agaaatatgc agacttcacg ggtaatcgct ctcatgcgtt tgtctcgctg
     1561 ctggaagcca ttcggcctct cgaactagtt caccgtagct atactgtcct gaaggggcgc
     1621 actgttgata ttgggcggcg cggtcactca gtgacggaag ggccgcagcg gatcatgaca
     1681 ttgattgctg gtggcgacga gtgggcagcc gttgctcttc ttgagaccgt cggtccacgc
     1741 gacgctgcga tcagggcctc ggcgttcgcg ctggggggaa tcaacgcgta tttcgaaagc
     1801 ttggacggtg agcttgatcc agagtatatg gtgcgtcaca tggccattac cgcggacacg
     1861 gcgattcgct acgttcgaac ccacgaagcc ttagagggac acgctctagg cggctcctgc
     1921 gcagtcgccg ttcaaacgcg aggcatcctt catactgccg cgcttggacg ggccggcgcg
     1981 ttgtgccact ggtcctcgtc aagcggaagt tgcttcctgc ctgtgcaaag cgatttcacc
     2041 gcgaaattag ttacgggcgc cggggaccca ggcttcgcct tgactcatcc ccttggctac
     2101 cttgcggacg gccgactgga gtttggggtc catccaactg agttccgaac ggcgggcgac
     2161 tttgttctgc tattgtcgcg cccactgccc gacaacgaac aggcgcgcgc tgaactctgc
     2221 tcagccctcg acggccgcgc cgatgggcac gtgttggtgg cacacgtatg caacaatcag
     2281 gcaccaccga cggacgactt catggtgctt gccctccgca ggtagttgct tggcccgcgc
     2341 caactggtta cggagtgaaa cgaagtgggc aggtctgaag tgtaaagtgc cgaataatgc
     2401 gcctgggctc gtgggtgctc tgacccgggc gctttcggct tccgaactga cccccgagga
     2461 gacggcccca acgatgatgt acacagcgga gcgcgaccac gggattgcgc gcggcttccc
     2521 caaggcgagt tcccgacgaa gactcgacat acaattcctc cgctcgtgag tatgaacgtc
     2581 tttgtgctag ctgggggtca ggtctaaagt ctaaagtgcc gaagacgatc gtcgctatgg
     2641 cgatcaggtc gagccggcac tttagacttt ggacctgacc cgccaacctc ctccgaccgt
     2701 gcacggtgcc gcacgttcgg atgcgttgtc gttcttcatg acgtcatgga acccgggcgc
     2761 gcttgtgtgc gcgcccgggt tcacaaactc ccctactcgg attccggagc cggcggcgcg
     2821 ggcggtggcg tttgagactt cttgcggcgc gcgacgccct tcagccgttt gacttcctcg
     2881 acgtgcggca cgagcactgc cctcgcgggg tccctggcga gctgcgaccc gatcgtgtac
     2941 gcctgcatgg cgaggagccc gagccgttga cggcgaatga gggccgctcc ctcgaggccg
     3001 ttcaggaacg cgcgggcctc ttcgaccgcg gcgtcccagc ggagcgattc gtcctgcaac
     3061 tgacggactt cgtcgagcgg ctccccgatc gcctgcgata cgttgtcgag cacgccgatg
     3121 acgttgatcg atgcctcgac gatggtcttc ggctgtctcc ggagacgctt tttgagtagc
     3181 gcgcgctgct ctttcgacaa cggcgcgagg tcgtcgatct gcgacccgat ggcgcgcaac
     3241 tgcgcgacga ccgcttcggg ggtcagcttt gccggatcag ggtttgatgg aacagcggtg
     3301 ttattgacgg tagtactcat gacatacctt cctcatgcga gccaggccgg aagtgacgtc
     3361 acggacttgt cgacaagtgc gagcggccac cggctggaag tcccggccac gcgccgattc
     3421 ttccggttga tgtttgcccg aggcgtgtac acacctcggg tgcccgcggc aggaggagca
     3481 agctcaactc ccgcgccggt acgggacaca tcatgctatc cagcacgccc gtaccgccga
     3541 cagccgccaa aataggagac cttgcggtct tcgactttgt aacgaaaggt taaggccggt
     3601 tgggacggcc tgtcaagggt cagcgcgcga cagcccgaag tgtaacgttt gcagcaacat
     3661 gcgcgcccag gcgcaccgag gtgaccgcat tgcggcgctt catggccaca cgtgcggcgc
     3721 tagttgaccg cattgcggct cttcctgtcc gcacctgcgg cgctagttga ccgcattgcg
     3781 gcgcttcctg tccgcacgtg cggcgctagt tgaccgcatt gcggctctgc ctgtccgcat
     3841 tgcggcgctg gttgaccgca ttgcggcgct ccctgtccgc acgtgcggcg ctagttgacc
     3901 gcattgcggc ccttcctgtc cgcacgtgcg gcgctagttg accgcattgc ggcccttcct
     3961 gtccgcacgt gcggcgctag ttgaccgcat tgcggcgctc cctgtccgca cgtgcggcgc
     4021 tagttgaccg cattgtggcc cttcatggcc gcgcgtgggg cgaaagatga cgccgttgct
     4081 gcacgctgtg tctgcactgc ggcccgaggt gacggcgttg ccgtccgccg tatccggact
     4141 gcgctccgat ggtgcaacgt tgcgtcgcgg cagaagcgca tatgcgctcc gagggtgcaa
     4201 cgttgcgaca cggcgtgcca cagtgcgcgc ggactggatg tcgtcgggtg gctgtggccg
     4261 ggttatgtcc tctcgatata ctctccatga tcctgagttt gcgcgacgga acctgtactt
     4321 cacacaaaca gaacgtcaac gacttcgctc tcccctgacc gccttcgggc gaggacgccc
     4381 gagggcccgc ggtcgagacg accgcacttc catgagaagg ccatcatctg tcaagtcgac
     4441 atcgtgagag cgccttggcg ctcgagcgat gtcgctctct tgcagttcgc tgttgtctct
     4501 ctggttgttg ctggtctcgt tgccggtccg atgaatcgag cgatccacac ttccatccgc
     4561 actctgagtg gtctccgtct gagaccggtg cactatgagg ttcgctccgt tcgagagttg
     4621 gggctgctat ctccttgtcg acctgtgccc gagggcacga tcctgtgcgg tatcgcagtc
     4681 acccgccttc gaattctcga ttcacgcggc cggcaatgcc gacctcaatg ctccttcctt
     4741 tgaacgattg acttccatct gctgatgcca cgttatgccg cgacggacag accgattccc
     4801 ttgctgctgt tgcgtagcgg ctcgtacgtc tcctggctaa cccataaccg atgcatgagc
     4861 accgagagct ttcgtgccac ggcgatggtc gctcgtcgtt tggctcgctt gcctcctcgc
     4921 tcggcgatcc ggatcccgtg ccggcgcaga tcgcagtcgt ctccgaatgg tccctggatg
     4981 tagtgcgcgc tctggatcaa tagccgtcgc atcaaccggt cgccggcctt ggtaatccct
     5041 cgctgcggat cgtcgtctcc tgattgatct cgtttactcg tcaggcccag gtacgctcct
     5101 acacttcggc tgcgccggaa ccgcgctgcg tcttcaatga ccagtacgaa cgccagcccc
     5161 gtcagcgctc ctactccctt gatctgggtg atccgttcca cttccggata ccgctcgttg
     5221 accattcgca agacttggcg gtcataggca gtgactgtct ttgtgagttg gtcgatttcg
     5281 gtgtagaccg gactgagtgc ttccttcagc gccgccggca ccagagcctt ggtcttgttg
     5341 acgaaggctt cggtcccaca cgccggcagc cgttcaccga acgacttgat cgctccccgc
     5401 acgtgactga tcaacttcgt tctcgactca accagcacca cacgcgtacg aatgatctga
     5461 agatcgatct gtgcctgctc gcctcgatgc cggatcgggt gcagcaactc gcggtcgaat
     5521 cgcgccagcc tggccagata ctcagcgtcg atcacgtcgc tcttccgatc gttcctcgtg
     5581 atcaaggcaa ccttcaccgg attggccacg atcacctcat ggccgcatgc cgtcagcaga
     5641 cggctgatcc acggcgaatg cgtccctgcc tcgatcgcga tcaccgtcgg cgcgattgct
     5701 cccgcccacc gctgcaactc aaccgcacgc aacgtcaccg ttccggtctc gatcgtcttt
     5761 ccctcgtcgt cgatcccatg gaaccgtcct gtccgatcac tcagatcaag ccccactgcc
     5821 gctaccccgt tcttcatcgt cttgttgctc acgctcttgg tagacttctt catggccaat
     5881 ctccttttct gcagatcctc tgtgctttca cactgaagat tactgcgcgg gggttggcct
     5941 tctcatctca tctccttgac cggcgcggcc ggctcggctc tggccactga acgtagaatc
     6001 gcgatcgtga agcggctact tcctctcttt ttcttctgtg catccgttgc ctttgcgggc
     6061 gacggcacgc tgcagatggc gactctcggc gatctcaagc tcgacagcgg gcaggtcata
     6121 cacgactgcc gcgtcggtta tcggacgtat ggcactctca aggccgaccg ctcgaatgtc
     6181 atcgtcgtca ccacctggtt tggtggcacg accgcggggc tgaccggttg gatcgggccg
     6241 ggcaagctgt acgactcttc caagtactac attatcgcca tcgacgccct cggcgacggg
     6301 gtttcgtcgt cgccgtcgaa cagcggcgcg cagcatgggg ccgactttcc gcccatctcg
     6361 atgcgcgacc tggtccgctc gcagcatcag cttctgacgc acg
//
LOCUS       DATLVW010000215         6371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_419653, whole genome shotgun sequence.
ACCESSION   DATLVW010000215 DATLVW010000000
VERSION     DATLVW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419653"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..960)
                     /gene="folK"
                     /locus_tag="VNN25_06890"
     CDS             complement(517..960)
                     /gene="folK"
                     /locus_tag="VNN25_06890"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXH91289.1"
                     /translation="MPFSVLVALGSNLGDRALSLRRAIDVLRGVIRVVRVSAFVDTEP
                     VEAPPGSPRFLNAVVAGYTDLDPQSLLAALLAIEARLGRRRTIRNAPRVIDIDLILHS
                     AHLVRTPGLTLPHARYLGRVFVREPLRTLGLSWRDPVTGESLNAT"
     gene            complement(960..1400)
                     /locus_tag="VNN25_06895"
     CDS             complement(960..1400)
                     /locus_tag="VNN25_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXH91290.1"
                     /translation="MNQKEFLAALDQPRIVAAVGAAERETSGEIRIHIQPKATGGDAR
                     YVAERTFERLGMTKTALRNGVLLFIASEERVFAIIGDKGIDEKVPAGFWDEIAAKLTL
                     RFGNGELTEGIIEAVTAAGHQLKAYFPRADDDVNELSDEIDVSD"
     gene            complement(1397..2182)
                     /locus_tag="VNN25_06900"
     CDS             complement(1397..2182)
                     /locus_tag="VNN25_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXH91291.1"
                     /translation="MNVRRSAIAFVAIALIASAALALDIPPKPTQWVNDYGANILSAS
                     DTQQLNEKLEGFYKKTGVQFLVMIFPSLEGEDDLGYTNKVANVWKVKDDKALMLFVFI
                     KEHKTRIQVGYSMEPVITDAFSSDVLHETVPPFFRAGDYAGGLNAAIDQIWKKIDPNA
                     VPAGSAPTAPRTRRSNGVGIPIRLIFYLILFVFFVVLPAITRRGRRSGCGGCFWPMMF
                     LGGGGGGTTFGGGGGGGGGGWSTGGSWGGGGSSFGGGGSGGGW"
     gene            complement(2179..2847)
                     /locus_tag="VNN25_06905"
     CDS             complement(2179..2847)
                     /locus_tag="VNN25_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXH91292.1"
                     /translation="MGKVGLLGCVGVLILVVVIFGAWFIGVRNHLVELDQQVQAQWAQ
                     VENDYQRRFDLIPNLVRTVQGAANFEKSTLEAVISARSRVGQVAAPAGAPGAQSNLPN
                     DPNAMAQFAGAQENLTGALSRLLVVVERYPDLKSNQNFLELQSQLEGTENRIAVERKR
                     YNDVAQQFNTAVLVFPANLVANMSGFKPKAYFKGAAGSQQAPEVNFNFGNTPTAPAPA
                     TGTR"
     gene            complement(2899..3213)
                     /locus_tag="VNN25_06910"
     CDS             complement(2899..3213)
                     /locus_tag="VNN25_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HXH91293.1"
                     /translation="MPDLGKKYTCYSCHTKFYDLGKPVPTCPKCGADQRDADEAPSVP
                     ASRSRARAAAAVVVEEPADEDFGADGEVATPPTGDEDEDIIPEADRGDEPAADDEEDE
                     EY"
     gene            complement(3402..5069)
                     /locus_tag="VNN25_06915"
     CDS             complement(3402..5069)
                     /locus_tag="VNN25_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXH91294.1"
                     /translation="MAKILKTHKWTFSLTLAVALIAATLFGGFYGNRLFGSPVQVDQK
                     RFREYTDLLNAVTMWAPDDVGADKFVYSSIDGMLRTLDPHTSFLEPKEYSDMQDRQKG
                     SFYGLGILVTKRNDQVTVITPLEGTPAARLGIRAGDVISEVEGVSTDDLPLDDVVKRL
                     KGPKGTTVHIKIVRVGMKEPIPLSIVRAAIPTNSISNVLMLKPGIGYIRIKDFTATTV
                     RELDEAIDKLKGEGMQKLVLDLRQNPGGLLDAAVGVSDHFLDKGQMIVFTKGRTPDSE
                     QNYLAPGKHQKLDMPMVVVVNRGSASASEIVAGALQDHDRAIIIGETSWGKGLVQSVY
                     TLQYGAGLALTTSKYYTPSGRNIQRDYTSFYDYYVADDSEDGSGSEVPLKDRKKFTTA
                     TGRVVYGGGGITPDIFVKPAPLSRTTQLLEVRSAIFNYGVDYYAKHPDVTQDVTITPQ
                     VMEDFVRFAADKDIAPLADIREALTKPNDRSFIERALKAEIIAAKFGFDASYPYRLQG
                     DNQIAKAIEVLPESQKLAEKAAALHAAPGANSTDAGTRAAQALPHGP"
     gene            5176..5517
                     /locus_tag="VNN25_06920"
     CDS             5176..5517
                     /locus_tag="VNN25_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatA/E family twin arginine-targeting protein
                     translocase"
                     /protein_id="HXH91295.1"
                     /translation="MFGSLGIPELILIFIVALIVFGPKRLPEIGRTLGKAMGEFKKAT
                     DDFKNTIEREVQVEELKQLASTTIPVHESVSRTEPAPAPPPPQVADVTPEAVAAVPHS
                     ELHTATGERTE"
     gene            5521..6303
                     /gene="tatC"
                     /locus_tag="VNN25_06925"
     CDS             5521..6303
                     /gene="tatC"
                     /locus_tag="VNN25_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXH91296.1"
                     /translation="MSDTAQASPALDGTPDSPEVELPKMSFLDHLEELRKRLIVSIIA
                     IGVAFLACWNFADKIYAWVEAPLTKLLPLGDRKLAYTHLTEPFMLYMKVAFFAAIFVS
                     SPIIMWQVWRFISPGLYKRERRMAAPFIIFATAFFLIGGYFGYRVILPGACAFFVETG
                     RQFKQMIKIDEYFGFASSIMLASGAIFETPILIFFLARLGIVTPAFLLQKAKWAIVLS
                     FIIAAILTPSPDMVNQTMLALPMIALYFLGVGVAFIFGKRHT"
BASE COUNT         1159 a         2045 c         1920 g         1247 t
ORIGIN      
        1 gtcccaacgc gcgcaacggc acccgcacaa actcccgccc gaggtaccgc ggatgcggca
       61 acgtcacggc cacgccaata ctgcatgccc acgtccacac gacgctgagc ggcgggcctg
      121 tcgcgacgcg gtaggttgaa cgtaggtcgg actctccagt ccgactcttt cgcccccaaa
      181 cgtcggactg gagagtccga cctacgttgc attcagcgat tcgcctgtga ccggatccgt
      241 ccacgacagt ccaagcgtcc gcaacggccc gcgcaaaact cccgcccgag gtaccgcgga
      301 tgcggcaacg tcaccccgtc gcatctccac ttcggcgtcg accagaatga cgagatggtc
      361 aacccctatc cgctcctgac ccgggccaca ccgacgctcc acgcgcacgt ccacacgaca
      421 ttgagcggcg ggcctgtcgc gacgaggtag gttgaacgta ggtcggactc tctagtccga
      481 ctctttcgcc ccaaacgtcg gactggagag tccgacctac gttgcattca gcgattcgcc
      541 tgtgaccgga tctctccacg acagtcccaa cgtccgcaac ggctcgcgca caaacacgcg
      601 tcccaagtac cgcgcatgcg gcaacgttag cccgggtgtc cgaacaagat gcgcgctgtg
      661 caggatgaga tcgatgtcga tcacgcgcgg cgcattgcgg atggtgcgac gccgcccgag
      721 gcgtgcttcg atcgcgagca gcgcggcgag gagtgactgc ggatcgagat cggtgtagcc
      781 ggcaacgact gcgttcagaa atcgcggcga tcccggcggc gcttcgacag gttcggtatc
      841 aacgaacgca ctaacgcgaa ccactcggat cacaccacga agcacatcga tcgctcgtcg
      901 aagcgacagt gcacgatcgc cgaggttcga gccgagggcg acgaggaccg agaaaggcat
      961 cagtcgctga cgtcgatctc gtccgacagc tcgttcacgt catcatcggc gcgagggaag
     1021 tacgccttga gctggtgacc ggcagcggtg accgcttcga tgatcccttc ggtgagctcg
     1081 ccattgccga accggagcgt cagcttcgcg gcgatctcat cccagaaccc ggccggcacc
     1141 ttctcgtcga ttcccttgtc gccgatgatc gcgaagacgc gttcctcgga tgcgataaag
     1201 agcagcacgc cgttccggag cgcggtcttc gtcatgccga gtcgttcgaa cgtacgctcg
     1261 gcaacatagc gggcgtcgcc gccggtcgct ttcggctgaa tgtgaatgcg aatctcgcct
     1321 gaagtctcgc gctccgcggc gccgaccgcg gcgacgatgc gcggctgatc gagcgcagcc
     1381 agaaactctt tctggttcac catccacctc cggagccgcc gccaccgaac gagctgccgc
     1441 cgccacccca tgagccgccg gtgctccatc cacctccgcc gccaccacca cccccaccga
     1501 acgtcgttcc gcctccgcca ccgccgagga acatcattgg ccagaagcag ccgccgcatc
     1561 cgctgcgccg gccgcggcgt gtgatggccg gtaggacgac gaagaagacg aagaggatta
     1621 ggtagaagat cagcctgatg ggaatgccca caccgtttga tcgtctggtg cgtggcgcag
     1681 tgggcgcgct accggccggc accgcgttcg gatcgatctt cttccagatc tgatcgatcg
     1741 cggcgttgag tccgcccgcg taatcgcctg cgcggaagaa tggaggaacg gtctcgtgca
     1801 gaacatccga cgaaaaggca tccgtaatca ccggctccat gctgtagccg acctggatgc
     1861 gcgtcttgtg ttccttgatg aagacgaaaa gcatcagcgc cttgtcgtct ttgaccttcc
     1921 agacgttcgc gaccttgttc gtgtatccga ggtcgtcttc accttcgagc gaagggaaga
     1981 tcatcaccag gaattgcacg ccggtcttct tgtaaaaacc ttcgagcttc tcgttgagct
     2041 gctgcgtgtc ggaggcgctg agaatgttcg cgccgtagtc gttcacccac tgcgtgggct
     2101 tcggcgggat gtcgagcgcg agcgcggcgg aggcgatcag cgcgatcgcg acgaacgcga
     2161 tcgcgctgcg gcggacgttc atcgagtgcc tgtggccggc gccggggccg tcggagtgtt
     2221 gccgaagttg aagttcactt caggcgcctg ttgcgagccg gccgcgccct tgaagtacgc
     2281 cttcggcttg aacccgctca tgttggcaac gaggttggcc gggaacacca gcacggcggt
     2341 attgaactgc tgcgcgacgt cgttgtagcg cttgcgctcc acggcgatgc ggttctcggt
     2401 tccttcgagc tgcgattgca gctcgagaaa gttctggttc gacttcaagt ccggatagcg
     2461 ctcgacgacg accagcaggc gggaaagagc gcccgtgagg ttctcctgcg ctcctgcgaa
     2521 ctgcgccatg gcattcggat cgttcggcag attggactgc gcacccggcg cgccggcggg
     2581 agcagccacc tgcccgaccc gcgaccgcgc actgatcact gcctccaggg tcgacttctc
     2641 gaagttcgcc gcaccctgca cggttcgcac gagattcggg atcagatcga agcggcgctg
     2701 atagtcgttc tcgacctgcg cccactgcgc ctgcacctgc tggtcgagct cgacgaggtg
     2761 gttgcgcaca ccgatgaacc acgcgccgaa aatcacgacc acgaggatca acactcccac
     2821 gcaccccagc agaccaactt tacccatgtg ttccgtcctt ctcgacggag accaaaactc
     2881 gggcctccgt tcgacgcact agtactcttc gtcctcttcg tcgtcggctg ctggctcgtc
     2941 gccgcggtcg gcctcgggaa tgatgtcttc gtcctcgtcg ccggtcggag gtgtggcgac
     3001 ctcaccatcc gcgccgaaat cttcatcggc cggctcttcg acgactaccg ctgcggcggc
     3061 acgtgcgcgg ctccgcgacg cgggaaccga cggtgcttca tcggcatcgc gctggtccgc
     3121 gccgcacttc gggcacgtcg gcacaggctt accgagatcg taaaacttcg tgtggcagct
     3181 gtaacacgtg tacttctttc cgagatcagg cataactcga gaatctcccc cagtgtcaaa
     3241 agcggcgaca cgttagcacc gcgactcatg tgtagcaagt agcggtcaaa gatgaaaaaa
     3301 ggttgcgagt gacgcggttg cgaggccgcg cggcactcct gccgcacttc ctcgcgaccg
     3361 cgcacctcgc aacctcggtg cggtgttttc tatcgttcga tttatggtcc gtgcggcagc
     3421 gcctgcgcag cgcgcgtgcc ggcgtccgtt gaattcgctc ccggcgcggc atgaagcgcc
     3481 gctgccttct ccgccagctt ctgcgactcc ggcaggactt cgatggcttt cgcgatctgg
     3541 ttgtcgccct gcaggcggta tggatacgac gcgtcgaaac cgaacttcgc ggcgatgatc
     3601 tccgccttca aagcgcgctc gatgaaggag cggtcgttcg gcttcgtcag cgcttcgcga
     3661 atgtccgcca gcggcgcgat gtccttgtcc gcggcgaaac ggacgaagtc ttccatcacc
     3721 tgcggcgtga tcgtgacgtc ctgggtcacg tcgggatgct tggcgtaata gtcgacgccg
     3781 tagttgaaga tcgcgctgcg cacttcgagg agctgcgttg tccgcgagag cggcgccggc
     3841 ttgacgaaga tgtcgggcgt gattccgccg ccgccgtaca cgacgcggcc ggtggccgtc
     3901 gtgaacttct tgcggtcctt cagaggcact tcgctgccgc tgccgtcttc gctatcgtcg
     3961 gcgacgtagt agtcgtagaa cgaggtgtag tcgcgctgga tgttgcggcc cgagggcgtg
     4021 tagtacttcg acgtcgtgag cgccaggccg gcgccgtact gaagcgtgta aacgctctgc
     4081 acgagacctt tgccccagct cgtctcaccg atgatgatgg cgcggtcgtg gtcctgcagt
     4141 gcgccggcga cgatctccga tgcggaggcg ctgccgcggt tgacgacgac gaccatcggc
     4201 atgtcgagct tctggtgttt gcccggcgcg aggtagttct gctcggagtc tggcgtacgt
     4261 cccttcgtga agacgatcat ctgccctttg tcgaggaagt gatcggagac gccgacggcc
     4321 gcgtcgagca gcccgcccgg gttctggcgc aggtcgagaa cgagcttctg catcccttcg
     4381 cctttgagct tgtcgatcgc ttcatcaagc tcgcggacgg tggtggcagt gaagtctttg
     4441 atgcggatgt agccgatgcc cggcttgagc atcagcacgt tggaaatgga gttcgtggga
     4501 atggccgcgc ggacgatcga gagcggaatc ggctccttca tcccgacgcg gacgatcttg
     4561 atgtggacag tggttccctt cggacccttg agccgtttga cgacgtcgtc gagcggaagg
     4621 tcgtcggtgg agactccctc gacctcggag atcacatcac cggcacggat accgagtcgg
     4681 gcggcaggag taccttcgag cggcgtgatc acggtgacct gatcgttccg cttcgtcacc
     4741 aggatgccga ggccgtagaa ggaacccttc tggcgatcct gcatgtcgga gtactctttc
     4801 ggctccagga aggaggtgtg cggatcgagc gtacggagca tcccatcgat cgacgagtag
     4861 acgaacttgt cggctccgac atcatcgggc gcccacatcg tcaccgcgtt caggagatcc
     4921 gtgtattctc gaaaccgctt ctggtcgacc tgaactggcg aaccgaagag gcgattgccg
     4981 tagaatccac caaagagtgt cgcggcgatc agcgcgacgg ccagcgtcaa cgaaaaggtc
     5041 cacttgtgtg tctttagtat ctttgccata tcgtcgtttg cctgattttc agggagcaga
     5101 actcattata ggcgcggagt attcgacagc ccggcgatgt tcacgcggcc gtatcaaact
     5161 aaggagaagt tcttcatgtt cggttcgctc ggcatcccgg agttgatcct cattttcatc
     5221 gtagccctga tcgttttcgg ccccaagcgc cttcccgaga tcggccgcac cctcggcaag
     5281 gcgatgggcg agttcaagaa agcgacagac gacttcaaaa acaccatcga acgtgaagtc
     5341 caggtcgaag agctgaaaca gttggcgtcc acgaccatac ctgtgcacga gtctgtatca
     5401 aggaccgagc cagcgccagc gccgccgcct cctcaggtcg ccgacgtcac acccgaagcc
     5461 gtagccgcgg ttcctcactc cgagcttcac actgccaccg gcgagcggac cgaatagccg
     5521 atgtccgata cggcacaggc ttcgccggcc ttggacggca ctcccgactc tcccgaggtc
     5581 gagcttccga agatgtcgtt cctcgatcac ctcgaggagc tgcggaaacg cctgatcgtc
     5641 tccatcatcg ccatcggcgt cgcctttctg gcctgctgga acttcgccga caagatctac
     5701 gcgtgggttg aggcgccgtt gacgaagctc cttccgctgg gagaccggaa gctcgcctac
     5761 acgcacctga ccgagccgtt catgctctac atgaaggtgg ccttcttcgc ggccatcttc
     5821 gtctcttcgc ccatcatcat gtggcaggtg tggcgcttca tctcccccgg cctgtacaaa
     5881 cgcgagcgcc gcatggccgc gccgttcatc atcttcgcca ccgccttttt cctcatcggc
     5941 ggctacttcg gctaccgcgt catccttccc ggcgcctgcg ccttcttcgt cgagaccgga
     6001 cgtcagttca agcagatgat caagatcgac gagtacttcg gcttcgccag ctcgatcatg
     6061 ctcgcttcag gagccatctt cgagacgccg atcctgatct tcttcctggc ccgcctcggc
     6121 atcgtgacgc cggccttcct gctgcagaag gccaagtggg cgatcgtgct gtcgttcatc
     6181 atcgccgcca tcctgacccc atcgccggac atggtgaacc agacgatgct ggcgctaccg
     6241 atgatcgccc tctacttcct tggcgtcgga gtagcgttca tcttcgggaa aaggcacacg
     6301 taggtgaagc agagatcaga gacaagagat cagagataga ggctgacttc cgatcttggg
     6361 tggagagact a
//
LOCUS       DATLVW010000216         8676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_343503, whole genome shotgun sequence.
ACCESSION   DATLVW010000216 DATLVW010000000
VERSION     DATLVW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343503"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="VNN25_06930"
     CDS             complement(<1..1861)
                     /locus_tag="VNN25_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HXH91297.1"
                     /translation="MRPIDWLVLIGTLVTIVSYGVWKGRHRKNLDGYLLAGRQLQWPT
                     IALSIMATQASAITFLSTPGQAYADGMRFVQFYLGLPIAMILLAIVAVPIYHRLKVYT
                     AYEYLETRFGPKTRVVTASLFLAQRGVSTGLTVYAPAVIVSLLLGWNIHVTNIGIGLL
                     VIAITVGGTKAVSRTHVHQLLIALAGMAVALTIAIHSLPADVSLFDALHVAGKMGRLN
                     VIDFSFDLKTPYNFWSGIIGGTLLMLSYFGCDQSQVQRYLTGETVQQSRLGLLINGMI
                     KVPMQFFILFVGAMVFVFFQFTAPPLFFNSAARNKVVKTRYASQYAVLEQTHAATFAS
                     KRNGMREMLAAMHTGDSARADAVATRVRALQTTDANTHKAAADLIAKADPAADTNDTN
                     YVFLSFVIGFFPAGVVGLLLAAIFCAAMSATASGLNALSSTSVVDVYRRFISCDANDR
                     DCVRMSKMLTVFWGLVSIGVGEFASHVGSLIEAVNRFGSFFYGTILGVFVLAFFFSKV
                     GDLAAVSGAVVGQIAVGLCAIFTNTAYLWWNVVGCVVGVVAALVIQTFMPDAGVADRV
                     SGVGASNLGWCNSDSRLPTTYSPKITPTPDPRKPTPATSLLPLRDRVADFRGER"
     gene            complement(1858..2130)
                     /locus_tag="VNN25_06935"
     CDS             complement(1858..2130)
                     /locus_tag="VNN25_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91298.1"
                     /translation="MSERGSGVGSRESSRESGAGGRGSEHGNDPTAAATPAASDNPAA
                     AAGSAPTPDPRQTTPAEPNWFLLYGIVVAELVVLIIAFNAFTKAFA"
     gene            complement(2127..4655)
                     /locus_tag="VNN25_06940"
     CDS             complement(2127..4655)
                     /locus_tag="VNN25_06940"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXH91299.1"
                     /translation="MLRRSIALLVILVAIPASLLAQKPLDAAQIQLALRKLTVVGSAL
                     YVAAHPDDENTALIAYLGNERLYRTGYLAMTRGDGGQNLLGDEKGELLGVIRTQELLA
                     ARRTDGSEQFFTRAIDFGFSKNPTETLAIWGHDTILADVVWNIRRFQPDVIITRFPTT
                     GEGGHGHHTASAILAGEAFTQAGDPTKFPEQLKYVGVWQPRRLFFNWFSFQVKPDDPT
                     LAKSLKLDLGTFNPLLGRSYTEIAAESRSMHKSQGFGAAERRGTAPNYFNQLAGDPAT
                     KDMFEGIDTTWSRYPGGEAVGKILQQASDTFDPMNPAKTIPLLLAAHDRLEQLSAGAS
                     WGRDPWPQVKLRDLQNVIKACAGLSIDVAASDSSVVAGGSIPVSVSVINRSDYPFTLS
                     MVASRYADPSKAVNAPLTNNVPVKTDLVIKLPADFPISQPYWLAKPPSKGTYTVDDQR
                     LIGVPENPPSIPIVVTLTDNAMHTIILDAPAVYRWTDAVLGERTRNVDVVPEVTANLG
                     SGVYVFPDTQPKPVTVWLRNFDGMGPVSVRLKFLNAAGSAAGPAPGWKSDPEAMVVVF
                     SKKGDEGHVTFMVTPPKAEMTTLLAAEAETKDGKKIDIGVTNIEYPHIPAQRIFGDAV
                     AKLVRVDVKKRGERVGYVMGAGDDVPSALRQVGYDVTLLTDDDLDRGDFAKYDAIVTG
                     VRAYNARPRMRLAHPKLMEYVKNGGTLVVQYNSTNPQPLLVDVPGPYPFKVTTERVTV
                     EEAPVRFVHPDSPLLNTPNKITQADFNDWVQERGLNFVKDWDPQYQTVLSSNDPGEPE
                     KEGGELYAHYGKGTFVYTSYAWFRQLPAGVPGAYKMFANLVSAK"
     gene            4818..5177
                     /locus_tag="VNN25_06945"
     CDS             4818..5177
                     /locus_tag="VNN25_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91300.1"
                     /translation="MPKRVSASRAALPRKRRNDLDEMFESVSPEAAALVARNPRLRAK
                     LTRAAERLADSFPGRALHVIRAFDHESGDRDLFVEIARAGDWREDYVRLREFVHSWIH
                     DREDPKVEVNFDFHFRQ"
     gene            5533..6756
                     /locus_tag="VNN25_06950"
     CDS             5533..6756
                     /locus_tag="VNN25_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HXH91301.1"
                     /translation="MPHSDPYDVAVIGAGAAGLMAAISAGRAARERGVPLRIAAIDSA
                     KKIGAKILISGGGRCNITNEVVRGQDFNGLQNGIAKVLRTFGVADTIAFFEDLGVLLK
                     REDTGKIFPISNRARDVVDALLAAAASVGVEIVSGMPVVSIEPGFAINGSIRADCVIL
                     AAGGRSVPKTGSDGSGYALARSLGHTVTTTFPALVPLIVRPGHWITTISGTSVDAELS
                     VKSPTGRVLHRHRGSMLFTHFGLSGPVVLDISRHWIANQPATLSANFLRETFDSLEAA
                     LLDAGRRNPRSTISSVLRGRLPERLLTQLAPDTPLGRMTKEDRRRIVRSVVDLTLPVI
                     RDRGFDYAEVTAGGVPLDEIDLATMESRICKNLYLCGEVLDVDGRIGGFNFQWAWASG
                     RAAGLSVALAKGGRR"
     gene            6856..7473
                     /locus_tag="VNN25_06955"
     CDS             6856..7473
                     /locus_tag="VNN25_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HXH91302.1"
                     /translation="MDDIRRRRLKVARLEELNDPFELLGADLRHPALRYAVVAAKSEF
                     ARVRGFLCFSLDWHNPVQWSHYASKHQGLCLGFEVPDDDCVRPITYSAKRFIVDVERL
                     LNMTTPDPVLFARFLFTKYAHWRYEKEVRAIVSLKESENGLYFSEFNDELQLACVIVG
                     ARSKVTRDMLRAALGNLAPQVEVFKARLAFRTFKVVRQRNPGLWA"
     gene            7622..>8676
                     /locus_tag="VNN25_06960"
     CDS             7622..>8676
                     /locus_tag="VNN25_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91303.1"
                     /translation="MSFDGFVYNVMIASPGDVANERTIAREVIAEWNAVHAPDRRLVL
                     LPISGDVNATPAMGVPPQEVINRQVLRAADLLVGIFWTRIGTATSSYASGTVEEIEEQ
                     IKAGKPVMLYFSETPVHPDVGATEQYLQLRSFKESLRSRGLFETFSSPAEFREKFNRQ
                     LQIKVNRDSYFVSKPLPSDMIAEELMVRLQSSIATFLELPRPNTTTADTETSYEVQAL
                     LALAHQTQGQLIGVSGALTAVGSEGLSARDKDLVEIAKFLIEDAITVGYGTFSSYALS
                     SGREIALLENLIKPPEELERITRRLQRTSARSDLQLRFSTQLGFSSIHFDKNVFESVF
                     YNVIHNAMKYAERSSVV"
BASE COUNT         1639 a         2544 c         2798 g         1695 t
ORIGIN      
        1 cgcgctcgcc gcggaaatcc gcgactcgat cgcggagtgg aagaagtgag gttgcgggag
       61 taggttttcg ggggtcgggg gtcggagtga tcttcgggga gtaggttgtc gggagtcggg
      121 agtcggaatt gcaccatccg aggttcgaag ctccgacccc cgacacccga tctgcgaccc
      181 ccgcatcagg catgaacgtc tgaatcacca gcgccgccac cacgcccacg acacacccga
      241 ctacgttcca ccagagatac gcggtgttcg tgaagatggc gcagaggccg acagcgatct
      301 ggccgacgac ggcgccggag actgcggcga ggtcgcctac tttgctgaag aagaacgcca
      361 gcacgaagac gccgaggatt gtgccgtaga agaaggagcc gaagcggttc acggcttcga
      421 tcagcgagcc gacgtgcgac gcgaactcgc cgacgccgat tgagaccaag ccccagaaga
      481 cggtcagcat cttcgacatc cgcacgcagt cgcggtcgtt ggcatcgcac gagatgaagc
      541 ggcggtagac atcgaccacg ctcgtcgagc tcagggcgtt gagtcccgag gcggtggcgg
      601 acatcgccgc gcagaagatg gcagccagca gcaggccgac gacgccagcg ggaaagaagc
      661 cgatgacgaa gctgaggaag acgtagtttg tgtcgttcgt atcggcggcc gggtcggctt
      721 tggcgatgag atcggcagcc gctttgtgcg tgttcgcgtc cgtagtttgc agcgcgcgca
      781 cgcgggtcgc gacggcatcg gcgcgcgcag agtcgccggt gtgcatggcc gcgagcattt
      841 cgcgcattcc gttgcgcttg gaggcgaacg tcgcggcgtg cgtctgctcg aggactgcgt
      901 attgacttgc gtagcgggtc ttgacgacct tgttccttgc cgcgctgttg aagaagagtg
      961 gcggcgcggt gaactggaag aagacgaaga ccatcgcacc gacgaagagg atgaagaact
     1021 gcatcggcac tttgatcatg ccgttgatca gaaggccgag gcggctctgc tgcacggtct
     1081 cgccggtcag gtagcgctgc acctgcgact gatcgcatcc gaagtacgag agcatcaaca
     1141 gcgtgccgcc gatgattcct gaccagaagt tgtagggcgt cttcaggtcg aaggagaagt
     1201 cgatgacgtt gagccgtccc atttttccgg ccacatggag cgcgtcgaag aggctgacgt
     1261 ccgccggcag cgagtgaatg gcgatcgtca gtgcaacggc catcccggct agagcgatga
     1321 gaagttgatg aacgtgcgtg cggctgaccg ccttcgtccc cccgacggtg atggcgatga
     1381 cgagcaggcc gatgccgatg tttgtgacgt gaatgttcca gccgaggagg agcgagacga
     1441 tgacggccgg cgcgtagacc gtgaggccgg ttgacacgcc gcgctgcgcc agaaacagtg
     1501 atgccgtgac gacgcgcgtc ttcggcccga agcgcgtctc gagatattcg tacgcggtgt
     1561 agaccttgag gcggtggtag atggggacgg ctacgatggc gaggagaatc atcgcgatcg
     1621 ggaggccgag gtagaactgc acgaagcgca tgccatcggc gtaggcctgg ccgggcgtgg
     1681 agaggaacgt gattgcgctt gcctgagtgg ccatgatcga aagcgcgatc gtcggccact
     1741 gcagttgccg gccggccaga aggtagccgt cgagattctt gcggtgacgc cctttccaga
     1801 cgccgtacga gacgatcgtg acgagcgtgc cgatcaaaac gagccagtcg atcggcctca
     1861 tgcgaatgct ttcgtgaatg cgttgaacgc gatgatgagg acgaccagct cggcgacgac
     1921 gatgccgtac agcaggaacc agttgggttc tgcgggggtc gtttgtcggg ggtcgggtgt
     1981 cggagcagaa cccgctgcag ccgcggggtt gtcggaggcc gcgggggtgg cagcggccgt
     2041 cgggtcgttg ccatgctccg acccccgacc cccggccccc gactcccgcg aggactcccg
     2101 actcccgact cccgaccccc gctcactcat ttcgccgata cgagattcgc gaacatcttg
     2161 tacgcgcccg gcacgccggc aggaagctga cggaaccagg cgtagctggt gtagacgaac
     2221 gttcccttgc cgtagtgggc atagagctcg ccgccttctt tttccggctc gccgggatcg
     2281 ttgctcgaga gcaccgtctg gtattgcgga tcccaatcct tgacgaagtt gagaccgcgc
     2341 tcctgcaccc agtcgttgaa atcggcctgc gtgatcttgt tcggggtgtt gaggagcggc
     2401 gagtcgggat ggacgaagcg caccggcgcc tcttcgaccg tcacgcgttc cgtcgtcacc
     2461 ttgaacggat acgggcccgg cacgtcgacg agcagcggct gcggattggt gctgttgtac
     2521 tgcacgacga gcgtgccgcc gttcttgacg tactccatca gtttggggtg cgcgagccgc
     2581 atgcgaggcc gggcgttgta ggcgcgaacg ccggtgacga tggcgtcgta cttggcaaaa
     2641 tcgccgcggt cgagatcgtc gtcggtcagc agcgtgacgt cgtatccgac ctgacgcagg
     2701 gcggacggaa cgtcatcgcc ggcgcccatg acgtagccga cgcgctcgcc gcgcttcttg
     2761 acgtcgacac gaacgagttt ggcgacggca tcgccgaaga tgcgttgcgc ggggatgtgc
     2821 gggtactcga tgttggtcac accgatatcg atcttcttcc catcctttgt ctccgcctcg
     2881 gccgccagga gcgtagtcat ctcagccttc ggcggcgtaa ccatgaacgt tacgtgaccc
     2941 tcgtcgccct tcttcgaaaa gacaacgacc atcgcctcgg gatcggactt ccatcccggc
     3001 gctggtccgg ccgcgctgcc cgctgcgttg agaaacttaa gccgcacgct gaccggaccc
     3061 atcccatcga aattgcgcag ccagactgtc accggctttg gctgtgtatc ggggaaaacg
     3121 tagacacccg acccaaggtt cgcagtcact tcggggacga catcgacgtt gcgcgttcgc
     3181 tcgcccagca ccgcgtcggt ccagcgatag acggcaggag catcgaggat gatcgtgtgc
     3241 atggcgttgt cggtgagcgt gacgacgatc ggaatcgacg gcggattctc aggcacgccg
     3301 atcaggcgct ggtcgtcgac ggtgtacgtc cctttgctcg gcggcttcgc cagccagtac
     3361 ggctgcgaga tcgggaaatc cgcgggcagt ttgatgacga gatcggtctt gaccggcacg
     3421 ttgttcgtca gcggcgcgtt caccgccttg ctcgggtcgg cgtagcgcga cgccaccatc
     3481 gacagcgtga acgggtagtc ggagcgattg atgacgctga cgctgaccgg aatcgatccg
     3541 cctgcgacga ccgaagaatc actggcggcg acgtcgatgg agagcccggc gcacgccttg
     3601 atcacgttct gcaggtcgcg cagcttcacc tgcggccagg gatcgcgtcc ccatgacgca
     3661 ccggcgctga gctgttcgag acggtcgtgt gccgccagaa gcagcgggat cgtcttcgcc
     3721 gggttcatcg gatcgaacgt gtcggaagcc tgctgcagaa tctttcccac cgcctcgccg
     3781 cccgggtagc gcgaccaggt cgtgtcgatc ccttcgaaca tgtctttggt ggctggatca
     3841 ccagcgagct ggttgaagta gttcggcgct gtgccgcgcc gctccgccgc gccgaagccc
     3901 tggctcttgt gcatgctgcg gctctccgcg gcgatctcgg tgtaggagcg gccgaggagc
     3961 gggttgaagg tgccgagatc gagtttcaga gacttggcga gtgttggatc gtcgggcttg
     4021 acctggaacg agaaccagtt gaagaagaga cggcgcggtt gccagacgcc gacgtacttg
     4081 agctgttcgg gaaacttggt tggatcgcca gcctgggtga acgcttcacc ggccaggatc
     4141 gcagacgcag tgtgatggcc gtgaccgcct tcgcccgtcg tcggaaagcg ggtgatgatc
     4201 acgtcgggct ggaagcggcg gatgttccag acgacgtcgg cgaggatggt gtcatgtccc
     4261 cagatggcca gcgtctcggt cggattcttc gagaagccga agtcgatggc gcgggtgaag
     4321 aactgctcgc tgccatcggt gcgccgcgcg gccagcagct cctgcgtgcg gatcacgccg
     4381 agcagctcac ccttttcgtc gccgaggagg ttctggccgc cgtcgccgcg cgtcatggcc
     4441 agatagccgg tccggtagag ccgctcgttg ccgaggtagg cgatgagggc ggtgttctca
     4501 tcgtcgggat gcgcggcgac ataaagcgcg ctgccgacga cggtgagctt gcgaagcgca
     4561 agctggatct gcgcggcatc gagcggcttc tgcgcgagga gcgaagccgg gatagcgacg
     4621 agaatgacga gaagagcgat cgatcgacga agcatggggg cgaatggtaa tggcttcgcc
     4681 ggtagaatcg tgtttcggtg ttgtcacgcg gggtttctca gacgtcacgc gaagccgcga
     4741 agacgcgaag ggggccgaat cttcgcgcct tcgcgtctcc gcgtgagaaa cctcttcgcc
     4801 gtacaatctc tgttgccatg ccgaaacggg tttctgcctc ccgcgccgcc cttcctcgga
     4861 agcgccgcaa cgatctcgac gagatgttcg aatcggtgtc gcccgaggcg gctgcgttgg
     4921 ttgcgcggaa tccccggttg agggcgaagc tgacgcgggc ggcggagaga ttggcggatt
     4981 cgtttccggg gcgcgccctg cacgtgatac gagccttcga tcatgaatcc ggagaccgtg
     5041 atttgttcgt cgagatcgcg cgagctggtg attggcgcga ggattacgtc cgtctgcgtg
     5101 agttcgtgca ttcatggatt cacgaccggg aggatccgaa ggttgaagtc aacttcgact
     5161 ttcatttccg acaatgagct ttgcctggga tgatctgctt cctctttctg aagcgttaac
     5221 aagtctcgac ggtgatatca atcgcgaggc gtgtcttcga actgcgatca gtcgtgctta
     5281 ctacgcagcc tttggaatgg cgcgccgcgg tgcacggact ctgcgcctgg cgacgcgaca
     5341 aagcgcagcc gagcatgggg aaatcgccgc gttttacgca acgcggtatg ggaagaccgg
     5401 cgaggagatt gccatcgcgc tgggccgtct cagaaaccgt cgcaaacgcc gcggattacg
     5461 atgatgactt tgcagatgtc gaggacatct gtctcctatc gatcgaagac gcgcgtgacc
     5521 tcctccgcct ccttgccaca ctctgaccct tacgacgtcg ccgtgatcgg cgcgggcgcc
     5581 gccgggctga tggccgccat ctctgctggc cgcgccgccc gcgagcgggg agttcctctg
     5641 cgcatcgccg ccatcgacag cgcgaagaag atcggcgcga agatcctcat ctccggcggc
     5701 ggacgatgca acatcacgaa tgaagtcgtc cgcgggcagg acttcaacgg cctgcagaac
     5761 ggcatcgcca aggtcctgcg cacgtttggc gtcgcggaca ccatcgcttt cttcgaagac
     5821 ctcggcgttc tgctcaagcg agaggacaca ggcaagattt ttcccatcag caaccgggct
     5881 cgcgatgtgg tcgatgcgct tctcgccgct gctgcctctg ttggcgttga gatcgtgagc
     5941 gggatgccgg tggtctcgat cgagccgggc ttcgccatca acggttcgat ccgtgccgac
     6001 tgcgtcatcc tcgcagcagg cggccgctcg gtcccgaaaa ccggcagcga cggttccggc
     6061 tacgccctcg cccgttccct tgggcacacc gtcacgacca cgttccccgc gctcgtcccg
     6121 ctgatcgtga ggccgggcca ctggatcacg acgatcagtg gcacgagcgt cgatgccgag
     6181 ctctccgtga agtcgccgac cggccgcgtg ctgcatcgtc accgtggatc gatgctcttc
     6241 acccacttcg gcctcagcgg cccggtcgtc ctcgacatca gccgccactg gattgccaac
     6301 cagccggcca cgttgtccgc gaacttcctc cgcgaaacgt tcgactcgct cgaagctgct
     6361 ctcctcgacg ccggacgccg caatccacga tcgacgatct ccagtgttct tcgcggccgt
     6421 ctgcccgagc gcctcctcac ccaactcgcc cccgacacgc ccctcggccg catgaccaaa
     6481 gaggatcgcc gccgcatcgt tcgcagcgtc gttgatctca cactccccgt tatccgcgac
     6541 cgcggcttcg actacgccga agtcaccgcc ggcggcgtcc cgctcgacga gatcgatctc
     6601 gccacgatgg agtcgcgaat ctgcaagaac ctctacctct gcggcgaagt cctcgacgta
     6661 gacggccgca tcggcggttt caacttccag tgggcatggg caagcgggag ggcggcgggg
     6721 ctttccgtgg cactggcgaa aggaggcaga cgctaaggag gttgcttccg ggcagctata
     6781 cttccgtggc tgggaacatt tatcgtctgg aaacttcaaa tgcgggtatt ccactttgtc
     6841 gaggaacagt ggggcatgga cgacatccgt cgccgaaggc tgaaggtcgc gaggttggag
     6901 gaactcaatg atccattcga gctactcggt gccgatctga gacacccagc gctccgatac
     6961 gctgtggtcg ccgcaaagag cgaatttgca agggttcggg gctttctttg cttcagcctg
     7021 gactggcaca acccggtgca gtggagtcac tacgcttcta agcaccaggg tttgtgtctc
     7081 ggattcgagg tgcccgatga cgattgtgtt cgaccgatca cgtattccgc gaaacgcttt
     7141 atcgttgacg ttgaacggct gctcaacatg acgacgcctg atccagtctt attcgcaaga
     7201 ttcctgttca caaaatatgc gcattggcgc tatgagaaag aagtccgagc gatcgtatcg
     7261 ttgaaggaaa gcgagaatgg cttgtatttc tctgaattca atgacgagct ccaactcgct
     7321 tgcgtgatcg taggagccag atccaaagtg actcgtgaca tgctgcgcgc ggcgcttggg
     7381 aacttggcac cgcaggtgga agtcttcaaa gcgcgcctgg cattcagaac gtttaaggtc
     7441 gttcgtcagc gcaatccggg gttgtgggcc tgatccctta ccgtgccgct ctatacgagc
     7501 ggacggcttg actctgtttt gtcgcgatgg agaataggat ggcatctggt ggagtggcac
     7561 taagccttgc gattactagg cggtctacgc cttccggatc taccggtagg gagtgtccag
     7621 agtgagcttc gatggcttcg tctacaacgt aatgatcgcc tcgcccggcg atgtcgcaaa
     7681 cgagcggacc attgcccgcg aggtaatcgc ggaatggaac gcagtccatg ccccggaccg
     7741 gcgattggtt ctccttccga tcagcggcga cgtgaacgca acgccggcta tgggggtgcc
     7801 tccgcaggaa gtgattaatc ggcaagtgct ccgcgctgca gaccttctcg tcgggatctt
     7861 ctggacgcgg ataggcactg ctacttcgtc atacgcgagc ggaaccgtgg aggagatcga
     7921 ggaacagatc aaggccggca agccggtgat gctctatttc tcggagacgc cggtacatcc
     7981 ggacgtgggt gcaactgagc aatacctgca acttcgctca ttcaaagagt cgttgcgttc
     8041 gagaggcttg ttcgagactt tcagctcacc ggctgagttt cgtgagaaat tcaacaggca
     8101 actgcagatc aaagtaaacc gcgattcata ctttgtctcg aaaccactac cgtcggacat
     8161 gatcgccgaa gagctcatgg tccgcctgca atcgtctatc gcgacatttc tggaactgcc
     8221 ccgaccgaat accacgacgg cagacacgga gacgtcgtac gaggtgcagg ctctgctggc
     8281 gcttgcacac cagacacagg gccagttgat cggagtttca ggtgccctaa cggctgtagg
     8341 cagcgaaggt ctttcggcga gagacaaaga ccttgttgaa atcgcgaagt tcctaatcga
     8401 ggatgcgatc actgttggct atgggacgtt ttcctcatat gcattgtcat cagggcgaga
     8461 aatcgcatta ctcgagaact tgataaaacc tccggaggaa ctggagagaa tcaccagacg
     8521 ccttcaacgc acttcggcgc ggtcagacct ccagttgcgg ttttcgacgc aactgggttt
     8581 ctcgtcgatc cacttcgata agaatgtctt cgaaagcgtt ttctacaatg ttattcacaa
     8641 cgctatgaaa tatgcggaga ggtcgtcggt cgtttg
//
LOCUS       DATLVW010000217        12946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_8250, whole genome shotgun sequence.
ACCESSION   DATLVW010000217 DATLVW010000000
VERSION     DATLVW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8250"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VNN25_06965"
     CDS             <1..577
                     /locus_tag="VNN25_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH91304.1"
                     /translation="ALRSNAVRRNLTNIDVVNTDLDEGALPDVQADGIWIRWVFAFVT
                     RPKELLARIARRLKPGGTIVVHEYFDYSTWRLAPRSEVFEEFVAAVMASWRDNGGEPD
                     IGLDLPRWMSELGLRLTRRHPIIDVISPSSFIWEWPKTFIEVGLARLVDLGRMTADQA
                     KQVRDEVASRAAAANTLMITPAVMEIIAVAE"
     gene            complement(700..2610)
                     /locus_tag="VNN25_06970"
     CDS             complement(700..2610)
                     /locus_tag="VNN25_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXH91305.1"
                     /translation="MQTLTLIHSESFPIGKYRDALSAASIASQAVPTLDQLSSDPGAL
                     RVILVDPAINNGRPSSLDGRTAVVGIGLDEEPKWLGEEGIYIDLPLNPSQASLLRGVK
                     RAYEAIHQKRRNQQIERQLGERTRELRELSEVGIALSTERDHSVLLTTILTKARELSR
                     ADAGSLYLLDEVDDEKMLRWKLAQNDSIKVEGFEEMVLPITRRSLAGWVALTGDTLAI
                     DDAYSLPPDAEYSINRSFDEKTGYLTRSILVFPMVTHVGELVGVLQLINRRKPGAPKK
                     LTAATVPEEVVPFDAETVELMRSLAAQAAVAVENNLLYKSIERLFEGFVTAAVTAIEQ
                     RDPTTSGHSFRVADLTVELARIVDRDDEQYRDTHFTSEQVREIRYASLLHDFGKVGVR
                     EQVLVKEKKLYPLQLETLRNRFEFAMKTAENESNKRKLDYLLHHGAEAYKEFGKRADE
                     ELRQHLEKMQKDFAFVTKSNEPTVLPEGEFQYLQQLAELEFGDKRRLLSDDEVRILSI
                     RKGNLDPAERTEIESHVTHTYNFLRKIPWTKDLSAVADIAFAHHEKLNGRGYPRKLTA
                     LEIPIQSRMMTVSDIYDALTANDRPYKRAISTDRALDILKMEVGDGLLDGSLVRLFID
                     AKIFERAGVLRT"
     gene            2780..4036
                     /locus_tag="VNN25_06975"
     CDS             2780..4036
                     /locus_tag="VNN25_06975"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HXH91306.1"
                     /translation="MAAYTTDTITPKHHDALVAWQKKHFPELQFLEDNWSKYYNQTPF
                     QLFAKVGKLASENINYGRMTGQPRFEQAGDMVGNMFYTARDIIRAQASTELGSIQQHR
                     LTLEEAPSDQMKMAVLRIMAEELRHGYQMFWVFDHDKSWKKPGHPDVAQDTIDELLAM
                     ELGNHVLDAFNIPFTNFLDNVVFATVIDLVGKYQLEMQKVFSYAPMARSMGPMLSEEG
                     FHIGSGRGFLRDLAVAAHNDTGRFSIDEIQRVLNMWVPRGVEMFGNERGGDTAIAFGF
                     KDRNNVTAQSEYYNEVREVLELVNIAVVQAKVKDTPAPEARTIVREVQDTRETVSGVR
                     PADLLFAPDMKFFRSRGMEEIAFMPYDIDGDMITENGKPVGGEKYAAYLSSVLPDYYV
                     GGTEFAKYREALLGREAPAPGASYGW"
     gene            4181..4645
                     /locus_tag="VNN25_06980"
     CDS             4181..4645
                     /locus_tag="VNN25_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91307.1"
                     /translation="MRLVIAADYPSPRMLRKLIPFIFLLAMACGRLKEPTAPGAGAGD
                     PIDPTATFTRVQNEIFTPTCGQLGCHDTLGRQEGMILSPGRAYAMTVGVASVEMPQLV
                     RVMPSDAANSYLYRKITGAGITGDRMPQGGPYLTDAQTKLVRDWIRRGAPND"
     gene            4671..5537
                     /locus_tag="VNN25_06985"
     CDS             4671..5537
                     /locus_tag="VNN25_06985"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HXH91308.1"
                     /translation="MPRWPAIASFFLLLSAFSLQAEDRYTPIPTVPLGDALLSLPTSH
                     IPTEGTWEVKFTHRFNQSIDQGSFSDRVHSLWGLDSNADVGIGFSYVISRDLQATFMR
                     SNASDDIESSLKYVVVQQAPAIPFSLALRGGMDWRTERDVVDRTSFFAQAIVSRQIGT
                     HAEIFAIPTYVTNAGRAVNGTQSVALFKHAFNVPVGAALMVRPGLSVVAELIPKNRDL
                     PSKMNGGDFGWALGLKRAIGGHYFEILLTNSNATHADQYVTSTYQGSPLNKSDLHLGF
                     NIELRFGRVRHR"
     gene            complement(5631..5798)
                     /locus_tag="VNN25_06990"
     CDS             complement(5631..5798)
                     /locus_tag="VNN25_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91309.1"
                     /translation="MGRPQVSVVKRQREQAKRERQQQKAEKREQRKNDPSDPNADIDM
                     ADLVVEGKEIL"
     gene            complement(5865..6068)
                     /locus_tag="VNN25_06995"
     CDS             complement(5865..6068)
                     /locus_tag="VNN25_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXH91310.1"
                     /translation="MRTQGNVKWFNDAKGFGFITSENGDDVFVHFSAIQGAGFRSLPE
                     GAAVEFDVVKGPKGLQAANVVAV"
     gene            6344..7357
                     /locus_tag="VNN25_07000"
     CDS             6344..7357
                     /locus_tag="VNN25_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXH91311.1"
                     /translation="MRCPECNRPYEAGAASCSNCGLLLLNLVPHRRGEDLATKKRRAA
                     DQLAPCKFCGSDIPMNSIRCRQCSEIVNDEFYRERAQRTRSRINHASWVAYIFGLGAL
                     LVFRPVGLVSIAAGLLLSILYYAIPVEPPASKNNPRPRLSLWQRIKQQVKLERVSIPI
                     PNFPKRKIVFIGTPLIAAIVGYSANVILLQQPVNAVIRDNAAFAGMEVSAHYQYYMVP
                     GVVVYDLRTIGIRQTPIDVHTAFLEFAKKVRDKRYQRVDLSYRGTTKFSIDGASFHKL
                     GDEYAKRNFDYVLYTFPRLFHRVGAPTTAPSSSVSDRDALLQFHRQWYGDDHLTRTVA
                     EGM"
     gene            7398..7661
                     /locus_tag="VNN25_07005"
     CDS             7398..7661
                     /locus_tag="VNN25_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXH91312.1"
                     /translation="MTENAMVIVYAPIDNDPVKLRDLLAENRRVVLTSNGKPAAVLLS
                     VDDYRSMQATISLAQDPAMLQHVMDAADEMSAPVPANAGVSDD"
     gene            7890..8192
                     /locus_tag="VNN25_07010"
     CDS             7890..8192
                     /locus_tag="VNN25_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91313.1"
                     /translation="MTLFLAYLGRLLILSGSISGVFGAIRMANHFTRNVDGVFDLGVA
                     LLNALAKGDTAKGLVAVSDEPEQRLTTLQGLGFVFLGFLLQAVGVLLDLMTFRLVH"
     gene            complement(8216..8824)
                     /locus_tag="VNN25_07015"
     CDS             complement(8216..8824)
                     /locus_tag="VNN25_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXH91314.1"
                     /translation="MQTTWFLFDLGNTIIKLAYERVLGAICAESSMSRDELVELLEEP
                     GAYRDMERGAVSFYEFYEFLCDHAGYSGSIRDFHLAWSDFFDGTMPGIEELLERVRRR
                     YSVAFLSNSNEIHAELIPRQFATVFEKNDIFVFSHRIKTAKPDPDFYRRALETVGATA
                     EQAIFIDDLLENVLAARALGMRAFQFNDARSLTMELEAEGLL"
     gene            complement(8876..10483)
                     /locus_tag="VNN25_07020"
     CDS             complement(8876..10483)
                     /locus_tag="VNN25_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXH91315.1"
                     /translation="MEPLETHIDQASDDFRRNAGALQTLVDELNQRLAAARQGGGPKY
                     VARHKEQGKVPPRERIAALLDPDTPFLELSPLAANGMYDDGAPAAGIITGIGRIQQRE
                     CLIIANDATVKGGTYFPLTVKKHLRAQEVALQNNLPCVYLVDSGGAFLPLQAEVFPDR
                     DHFGRIFYNQAIMSAREIPQIAAVMGSCTAGGAYVPAMSDEAVIVKSTGTIFLGGPPL
                     VKAATGEEVTAEELGGADVHTRISGVADHFAEDDYDAIAIVRNIVENLPLDKKEPPTR
                     IEPEDPKFDPKEIYGVIPSETRKPYDVREVIARIVDGSKFQEFKERYAPTIVTGFARI
                     FGYQVGILANNGVLFSESALKATHFIELCNQRRIPLIFLQNITGFMVGKEYERGGIAK
                     DGAKMVHAVANSTVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQA
                     AGVLTTVKRDQLAREGKEFSVDDERALADPVLRKYDTEGSPYYSTARLWDDGVIDPAE
                     TRNYLALGLSIAYNAKIEDPRFGVFRM"
     gene            complement(10540..11334)
                     /locus_tag="VNN25_07025"
     CDS             complement(10540..11334)
                     /locus_tag="VNN25_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91316.1"
                     /translation="MKNDNVVLIVNDNLETISELRNRFMDAGLTVETAGESLAAIEKL
                     RTNSYPAIVLDPMIRHGLNGFAVLNYIELEQPETLSHVFLLTAMSRQTIIRTAPGLIP
                     RFFRSPREVQLAADAVIASIERQSVRERPKRSVLLIEDDSQTARVTSDALEQLGYTCE
                     WLPGGCKVLETVTASRFDAIMLDLIMPDVDGFAVLESLRSRKPELLRRVVITTGMPAK
                     YLDAIDRSRICGVVQKPVDLGELRHLLHRCADVVLFEAGGESPVAG"
     gene            complement(11331..>12946)
                     /locus_tag="VNN25_07030"
     CDS             complement(11331..>12946)
                     /locus_tag="VNN25_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH91317.1"
                     /translation="DGSILEAEIHGSVIYVDNRRIIIGAAVDVTGQAAINRRVKEREE
                     YFRALTENVSDVIAILDTEGRITYVSPSAERVLRYSTEELIGSSHFALVHPDDRDRVI
                     ASFQRLVAAGRNPVSCEPYRLRRKDGAWRLLESVGTNLLHHPQVQGLVISTRDVTERK
                     ALEQELEQLHRLTSLGRLSAQVAHEFNNVLMGIQPVVEVIRRYGANNPRLLRFSDLVA
                     ASISRGKRITTDILRFGRPAQLTLHPVKVDDLIRQVSEEIRPMLPDSIALSVDAGEVP
                     QDVTVSADRAQLSQVLINLALNARDAMLASGGTLTLGLSPDQPRELQQRFIHFTVTDT
                     GEGIASENLPHIFEPLFSTKKIGTGLGLSVVFQIVAAHRGHITVESEPGIGSTFHLYI
                     PSCEETGPQAEPDAPHEETVLPDTLHVLLVEDDEAVACGLQWSLEAEGIDAHVVGKGA
                     DVVPAMFKLQPDVIVLDLNLPDDDGRKVYERIAAQSRVPVIFSSGHAFEREIRELLNN
                     PRVAFLMKPYSSEELLRTIHQVLHAKESV"
BASE COUNT         2391 a         4007 c         3980 g         2568 t
ORIGIN      
        1 tgccctgcgg tccaacgccg tacgcaggaa cctgacgaac atcgacgtcg tcaacaccga
       61 cctcgatgag ggcgcgctgc ccgacgtaca agccgacggc atctggatac gctgggtctt
      121 cgcatttgtc acgcgtccga aggagcttct cgccaggatc gcccgcaggc tgaagccggg
      181 cggcacgatc gtcgttcacg aatacttcga ctactccacg tggcgcctcg ctccccgctc
      241 ggaggtattc gaggagttcg tggcggcagt catggccagt tggcgagaca acggcggcga
      301 gcccgatatc ggcctggacc tacctcgctg gatgagcgaa ttgggcctgc gcctgaccag
      361 gcgccatccg atcatcgacg tcatctcccc ctccagtttc atctgggagt ggccgaagac
      421 cttcatcgag gtcggcctgg cccgcctggt cgatctcggg cgcatgacag cggatcaagc
      481 gaagcaagtc cgcgatgagg tcgcgagccg agctgccgca gcgaacactc ttatgatcac
      541 ccccgcggtc atggagatca tcgccgtagc agaatgagcc tgagttggag ttggaggatt
      601 tcggctagcg gatcggaaaa ccattgcacc acagagacac tgaggacaca gagagttctc
      661 tgtgctctct gtgtctctgt ggtgacaaag ggttatggat tacgtccgca aaacacccgc
      721 gcgctcgaag attttggcgt cgatgaagag gcggaccagg gagccgtcca ggaggccgtc
      781 gccgacttcc atcttcagga tgtcgagggc gcggtcggtg gagatggcgc gtttgtaggg
      841 gcggtcgttt gcggtgaggg cgtcgtagat gtcggagacg gtcatcatgc gcgactggat
      901 cgggatctcc agcgcggtca gcttgcgcgg atagccgcgg ccgttgagtt tctcgtgatg
      961 ggcgaaggcg atgtcggcca cggcagacag atccttcgtc caggggatct tgcgcaagaa
     1021 gttgtaggtg tgggtgacgt gactctcgat ctcggtccgc tccgccggat cgaggttgcc
     1081 cttgcggatg gagaggatgc gcacctcgtc gtcgctgagc aggcgccgct tgtcgccgaa
     1141 ctccagttcg gcgagctgct gcaggtactg aaactcccct tccggcaaaa ctgttggttc
     1201 gttcgacttc gtgacgaaag cgaagtcctt ctgcatcttc tccaagtgct gccgcagctc
     1261 ctcatccgcg cgtttcccga actccttgta cgcctcggcg ccgtgatgca gcaggtagtc
     1321 gagcttgcgc ttgttcgact cgttctccgc cgtcttcatg gcgaactcga agcggttgcg
     1381 cagcgtctcg agctgcagcg gatagagctt cttctccttg accagcacct gctcgcgaac
     1441 gccgactttg ccgaagtcgt ggaggagcga ggcgtagcgg atctcgcgca cctgctcgga
     1501 ggtgaaatgc gtgtcgcgat actgctcgtc gtcgcggtcg acgatccgcg ccagctccac
     1561 ggtgaggtcg gcgacgcgga acgagtgtcc tgaggttgtc ggatcgcgct gttcgatggc
     1621 ggtgaccgcc gcggtgacga agccttcgaa aagcctctcg atcgatttgt agagcaggtt
     1681 gttctcgacg gcgaccgcgg cctgcgccgc cagcgaccgc atcagctcaa cggtctccgc
     1741 atcgaacggg acgacttcct ccggcacggt ggccgcggtg agcttcttcg gagcgccggg
     1801 cttgcggcga ttgatgagct gcagcacgcc gacgagctca ccgacgtgcg tgaccatcgg
     1861 aaagacgagg atggatcggg tcaggtaacc ggtcttctcg tcgaaagaac ggttgatcga
     1921 atactcggcg tccggaggca gcgagtaggc atcgtcgatg gccagcgtgt cgccggtgag
     1981 cgctacccac ccggccagac tgcggcgcgt gatggggagg accatttcct cgaagccttc
     2041 gaccttgatg gaatcgttct gggccagctt ccagcgaagc atcttctcgt catcgacctc
     2101 gtcgagcaga taaagagagc cggcatcagc gcgggataac tcgcgtgctt tcgtgaggat
     2161 ggtcgtcagc agcacggagt gatcgcgctc ggtggagaga gcgattccaa cttcgctgag
     2221 ctcgcgcaac tcgcgcgtgc gctcgcccag ctgcctttcg atctgctgat tgcgccgctt
     2281 ctgatggatc gcctcatacg cgcgcttgac tccgcgcaac agcgaagcct gcgagggatt
     2341 gagagggagg tcgatgtaga tcccctcttc gccgagccat ttcggttctt catcgagacc
     2401 gatcccgaca acggccgtgc gtccgtcgag cgacgatggc cgtccgttgt tgatggcggg
     2461 atcgacgaga atcacccgca gcgcacccgg atcggatgaa agctggtcca gcgtgggaac
     2521 ggcctgggaa gcgatcgatg ccgccgacaa cgcgtcgcgg tactttccga tcggaaagct
     2581 ctcggagtgg atcagcgtca gcgtctgcat gaagtggcgt ccgttggttc aaccggggtt
     2641 ccgctcgatc ttccatgcgg atgagtaacc gcttacaatc cgcgcgccat cgaattatgg
     2701 caccgatccg gcgagaatgt tggttcgctg ggagacgttg acgcgtgctg cggcaacccg
     2761 gcttttctca ggaggattct tggcagccta cacgaccgac acgatcacac cgaagcacca
     2821 cgatgccctc gtggcgtggc agaagaagca cttcccggag ctccaattcc tcgaggacaa
     2881 ctggtcgaag tactacaacc agacgccgtt ccagctcttc gccaaggtcg ggaagctggc
     2941 ttccgagaac atcaactacg gacgcatgac cggccagcct cgcttcgagc aggccggcga
     3001 catggtcggc aatatgttct acacggcgcg cgacatcatc cgcgcgcagg catcgacaga
     3061 gctgggatcg atccagcagc atcgtctgac tctcgaagaa gcgcccagcg atcagatgaa
     3121 gatggccgtg ctccgcatca tggccgagga gctgcggcac gggtatcaga tgttctgggt
     3181 cttcgatcac gacaagagct ggaagaagcc gggccatccc gacgtggcgc aggacaccat
     3241 cgatgagctg ctggcgatgg agctcggcaa ccatgtcctc gatgccttca acatcccgtt
     3301 cacgaacttt ctcgacaacg tcgtcttcgc cacggtcatc gatctcgtcg gcaagtacca
     3361 gctcgagatg cagaaggtct tctcgtacgc gccgatggca cgctcgatgg gaccaatgct
     3421 ctccgaggag ggattccaca tcggcagcgg gcgcggattc ctgcgcgatc tggctgtggc
     3481 cgcgcacaac gacaccggcc gcttctcgat cgacgagatc cagcgcgtac tgaacatgtg
     3541 ggtcccgcgc ggcgtagaga tgttcggcaa cgagcgtggc ggtgataccg caatcgcctt
     3601 cggattcaag gaccgcaaca acgtcaccgc acagagcgag tactacaacg aagtacgcga
     3661 ggtgctcgag ctggtgaaca tcgccgtggt gcaggcgaag gtgaaggaca ccccggcccc
     3721 cgaggcgcgg acgatcgtgc gcgaggtgca ggacacgcgc gagacggtca gcggcgtccg
     3781 tcccgccgat ctcctcttcg cgcccgatat gaagttcttc cgctcgcgcg gcatggagga
     3841 aatcgccttc atgccctacg acatcgacgg cgacatgatc acggagaatg gcaaaccggt
     3901 cggcggcgag aagtacgcag cgtacctgag ctccgttctc cccgattact acgtcggcgg
     3961 cacggagttc gcgaagtacc gcgaagcgct cctcggccgc gaagctccgg cgccgggcgc
     4021 cagttacggg tggtagatct ttcagaaccg tttcacgcgg agacgcggag gtcgcggaga
     4081 gaacacctcc gcatctccgc ggctccgcgt gaaacggttt tcctgatctc ttatctctga
     4141 tcgcttgtct ctgctttcct cctgttttca aacttctgac ttgcgattag tcatcgctgc
     4201 cgactaccct tccccgcgca tgctccgcaa attgatcccg tttatcttcc tgctcgccat
     4261 ggcctgcggc agattgaaag aaccgaccgc accgggcgcc ggcgccggtg atccgatcga
     4321 tccgaccgcg acgtttaccc gggtgcagaa cgagatcttc actcccacct gcggccagct
     4381 cggctgccac gacaccctcg ggcgtcagga agggatgatc ctctcgcccg gccgcgcgta
     4441 cgcgatgacc gtcggcgtgg ccagcgtcga gatgccgcaa ctggttcgcg tcatgcccag
     4501 cgatgcggcc aattcgtacc tctaccgcaa gatcacgggc gcggggatca cgggcgaccg
     4561 catgccgcag ggcggaccgt atctcaccga tgcgcagaca aagctcgtac gggactggat
     4621 ccgccgaggt gctccgaatg attaagattc ctggttgctc cgttgctcgg ttgcctcgtt
     4681 ggccggcaat cgcatccttc ttccttctgc tctctgcctt cagccttcag gccgaggatc
     4741 gctacacacc gatccctacc gttcctctcg gcgatgcgct gctttccctt cccacttcgc
     4801 atatccccac cgaggggacg tgggaggtga agttcacgca tcggttcaat cagtcgatcg
     4861 atcaggggag tttcagcgac cgcgtccact cgctttgggg actcgacagc aacgccgacg
     4921 tcggcatcgg cttctcgtac gtcatcagcc gtgatcttca ggcgacattc atgcgcagca
     4981 acgccagcga cgatatcgag tcatcgttga agtacgtcgt cgtgcagcag gcgccggcga
     5041 ttccgttctc gctcgcgctg cgcggcggga tggattggcg caccgagcgc gacgtggtcg
     5101 atcgcacgtc gttcttcgcg caggcgatcg tctcgcggca gatcgggacg catgcggaga
     5161 tcttcgcgat cccgacgtac gtgacgaacg cagggcgggc ggtcaacgga acgcagtccg
     5221 tggcgctctt caagcacgcg ttcaatgtgc cggtgggggc cgcgctgatg gtgcggccgg
     5281 ggctgagtgt cgttgcggag ctgattccga agaatcgcga tctgccctca aagatgaacg
     5341 gcggcgattt cggttgggcg ctcggactga agcgcgcgat cggcggtcac tacttcgaga
     5401 tcctgctgac gaactcgaat gcgacgcatg ccgatcagta cgtgacgtcg acctaccagg
     5461 gctcgccgct gaacaagagc gacctccacc tgggattcaa catcgagctg cggttcgggc
     5521 gcgtgcggca ccgctgaagc gcagagtgtc ggaggacgtc gggggtcggg tgtcggagag
     5581 cacgctagcc agcaatctcc gactcccgac gcccgacaac cgacacccgc ctagaggatt
     5641 tctttccctt ccacgacgag gtcagccata tcgatgtcgg cgttcggatc cgacggatcg
     5701 ttcttccgct gctcccgttt ctctgctttc tgctgctgac gctcgcgctt ggcctgctcg
     5761 cgctgacgct tgacgacgga tacctgtggt cttcccataa acctccacac aacgaaaagg
     5821 gcaggatcgc tcctgccctt tcgtcgatcg ataagatcgg ctacttagac tgccacgacg
     5881 tttgccgcct gcaggccttt cggccctttg acgacgtcga actcaaccgc tgcaccttcc
     5941 ggaagcgacc ggaatccggc gccctgaatc gctgagaagt ggacgaatac gtcgtctcca
     6001 ttctcgctgg tgatgaaccc gaaacctttg gcgtcgttaa accacttcac attgccttgt
     6061 gttctcatct tgaatctcct ttctgtgctg ccatcggaga ttcaaaaacg gtgttcttcg
     6121 tctcgataag gcaaatcacg gatgcacagg cgaaccggca gggatggcag gctattccga
     6181 atgggagtcg tgggccggga gtcgggggtc ggagataagc aaagcgagct ggggctctct
     6241 ccgacacccg actcccgaca cgcgactccc gttcacggag aattctgaga cgacgcggca
     6301 acccggcatt atggcggccc gtttgcagtt attgaggcca accatgcgct gcccggaatg
     6361 caaccgaccg tacgaagctg gtgcagcctc ctgttccaat tgcggcctgc tcctcctgaa
     6421 tctcgttccc catcgccgcg gcgaggatct ggcgacgaag aaacgccgcg ccgcggatca
     6481 gctcgctccc tgcaagttct gcggaagcga cattccgatg aattccatcc gctgcagaca
     6541 gtgctccgag atcgtgaatg acgagttcta ccgtgagcgc gcgcaacgaa cgcgctcccg
     6601 catcaatcat gcgagctggg tggcctacat cttcgggctg ggcgccctcc tggtgttccg
     6661 cccggtcggc ctggtatcga tcgcagccgg actccttctc tccattcttt actacgcgat
     6721 ccctgtcgaa ccgccggcgt cgaagaacaa tccgcgcccg cgcctctcgc tctggcagcg
     6781 catcaagcaa caggtgaagc tcgagcgggt gtcgattcca attccgaact tcccgaaacg
     6841 gaagatcgtc ttcatcggca cgcctctgat cgccgcgatc gtcggctact ccgcgaacgt
     6901 catcctgctc cagcaaccgg tgaacgccgt catacgcgat aacgcggcct tcgctgggat
     6961 ggaagtgtcg gcgcactacc agtactacat ggtcccgggc gtggtcgttt acgatctgcg
     7021 caccatcggg atacggcaaa cgccgatcga tgtgcacaca gccttcctcg agttcgcgaa
     7081 gaaggtccgc gacaagcgct atcagcgcgt cgatctctcc taccgcggca cgacgaagtt
     7141 ctcgatcgac ggcgcgtcgt tccacaagct cggcgacgag tacgcgaagc gcaacttcga
     7201 ctacgtgctc tacacgttcc cccgcctctt tcatcgcgtg ggcgcgccta cgaccgcacc
     7261 gtcgtcatcc gtgtccgacc gcgacgcgct gctgcagttt catcgccagt ggtacgggga
     7321 tgatcacttg acgcgaacag tggccgaggg gatgtaaggg ctgcagaaag gcgctggcca
     7381 gcgtattatt ggcggacatg acggagaatg cgatggttat cgtctacgcg cctatcgaca
     7441 acgatccagt caaactcagg gacctcctcg cggaaaaccg ccgtgtcgtc ctcacgtcga
     7501 atggtaagcc ggctgccgtg cttttgagcg tcgacgacta ccggtcgatg caggctacga
     7561 tttcgttggc ccaagatcct gcgatgttgc agcacgtgat ggatgccgcc gacgagatga
     7621 gcgcgcccgt accagccaat gccggcgttt cagacgatta gctacgaccc gatcgtcctc
     7681 gccctagttg gccgcgacga gcggatcgcc gcctgcttgg tccccgtgtt tcaacaaatc
     7741 aagtccggaa cctggccgct tctgcgcgtg caagtcaggc aacagatcta ttggacagat
     7801 gcgtgcggcg tacgtttgct tatcgtggtc tcgccggatt cgcgccacgc acgtatcagc
     7861 ggctatcaaa tcgtgagggc tattgaacca tgacgctttt cctggcgtat ctcggccgcc
     7921 tgctcatcct ctcgggatcg atctcaggag tattcggggc gattcggatg gcgaaccact
     7981 tcacccgcaa cgtcgatgga gtgtttgatc ttggagtggc acttctcaac gctctggcca
     8041 agggcgacac ggcaaagggg ctggttgctg tttccgacga gccggaacag aggcttacga
     8101 cgctgcaagg tctgggcttc gtcttcctgg gattcctact gcaagccgtt ggcgtcctgc
     8161 tcgacctgat gacgttccgt ctcgtgcact gagagcaggc gcggaaccgg cctgattaca
     8221 gcagcccctc cgcctccaac tccatcgtca gcgaccgcgc atcgttgaac tgaaacgctc
     8281 gcatcccgag ggcgcgcgct gccagcacgt tctccagcag gtcgtcgatg aagatcgcct
     8341 gttctgccgt tgcgcccacc gtttccagcg cgcgtcgata aaaatcggga tccggtttcg
     8401 cggtcttgat gcggtgcgag aagacgaaga tgtcgttctt ctcgaagacc gtggcgaatt
     8461 ggcgggggat cagctcggcg tggatctcgt tggagttcga gaggaaggcc acgctgtaac
     8521 gccggcgcac gcgctccagc agttcctcga tccccggcat cgttccgtcg aagaagtccg
     8581 accaggcgag gtgaaagtcg cggatcgagc cgctgtagcc ggcgtgatcg cacagaaact
     8641 cgtagaactc atagaaggag accgcaccgc gctccatgtc gcgatacgcg cccggctcct
     8701 ccagcagctc gaccagctcg tcgcgcgaca tcgatgactc cgcgcagatc gctcccagca
     8761 cgcgctcgta ggcgagcttg atgatcgtgt tgccgaggtc gaagaggaac caggtggttt
     8821 gcatggttac tgttagctgc gttgatgggt tatgagggtc gccgagccat gcaactcaca
     8881 tcctgaacac tccaaaccgt ggatcctcga tctttgcgtt gtatgcgatc gacaggccga
     8941 gggcgagata gttgcgcgtc tcggcggggt cgattacccc gtcgtcccag agtcgggcgg
     9001 tcgagtaata cggcgaacct tccgtgtcgt acttccggag aacgggatcg gcaagcgctc
     9061 gttcgtcgtc gaccgaaaac tccttcccct cgcgcgcgag ttgatcgcgt ttgacagtcg
     9121 tcaggacgcc ggccgcctgc tcgccgccca tcacgctgat ccgtgcgttg ggccacatcc
     9181 agagaaagcg cggcgagtag gcgcggccgc acatgccgta gttcccggcg ccgaaggagc
     9241 cgccgataat gacggtgaac ttcggcacgg ttgagttagc gaccgcgtga accatcttcg
     9301 cgccatcttt agcgatgcca ccccgctcgt actctttgcc gaccatgaag ccggtgatgt
     9361 tctgcagaaa gatcagcggg atccgtcgct gattgcacag ttcgatgaaa tgcgtcgcct
     9421 tgagcgcgct ctcggaaaac agcacgccgt tgttggcgag gatgcccacc tgataaccga
     9481 agatgcgcgc gaagccggta acgatcgtcg gcgcgtagcg ctctttgaac tcctgaaact
     9541 tcgagccgtc gacgatgcgc gcgatgacct cgcgcacgtc atacggcttg cgcgtctcgc
     9601 tcgggatcac accgtagatt tctttcggat cgaacttcgg atcttccggc tcgatccgag
     9661 tcggaggttc tttcttatcc agcggcaggt tctcgacgat gttcctcacg atcgcaatgg
     9721 cgtcgtagtc atcctcggcg aagtgatccg ccaccccgct gatcctcgtg tgcacgtccg
     9781 ccccgcccaa ctcctcggcg gtgacttcct cgccggtcgc cgccttcacc agcggaggtc
     9841 cgccgaggaa gatcgtgccc gtacttttga cgatgacggc ttcgtcggac atcgccggga
     9901 cgtacgcgcc gcctgcggtg cacgatccca tcacggcggc gatctgcgga atctcgcggg
     9961 ccgacatgat ggcctggttg tagaaaatgc gcccgaagtg atcgcgatcc ggaaacacct
    10021 cggcttgcag gggcagaaac gcgccgccgg agtcgacgag gtagacgcac ggaagattgt
    10081 tctgcagcgc cacttcctgc gcgcgcagat gcttcttcac ggtcagagga aagtacgtgc
    10141 cccctttgac cgtggcgtcg ttggcgatga tgagacactc gcgctgctga atgcggccga
    10201 tgccggtgat gatgcctgcg gcgggcgcgc cgtcgtcgta catgccgttg gcggcgagcg
    10261 gcgagagctc gaggaagggc gtgtcgggat cgagcagcgc ggcgatccgt tcgcgcggcg
    10321 gaacctttcc ctgttccttg tgacgcgcca cgtacttcgg cccgcccccc tgccgcgccg
    10381 ctgccagacg ctgattcagc tcatcgacga gcgtttgcaa cgcgccggca ttgcgccgaa
    10441 agtcgtccga cgcctgatcg atgtgtgttt cgagcggttc catcctgagc gggcgcagcg
    10501 tacatgggcg tggcgccagc cgcaaccgga gcgcggcgat cagccggcaa cgggcgattc
    10561 tcctcccgct tcgaacagca caacatcggc gcaacgatgc agcaagtgcc ggagctcgcc
    10621 gaggtccaca ggcttctgca cgacgccgca gatccgcgag cgatcgatcg catcaagata
    10681 cttcgccggc attcctgtcg tgatgacaac ccgccgcaga agttcaggct tgcgcgaccg
    10741 gagactctcg agcacggcga agccatcgac gtcgggcatg atcagatcga gcatgatcgc
    10801 gtcgaaccgc gacgcggtta cggtttccag caccttgcag ccgcccggca gccattcgca
    10861 cgtgtacccg agttgctcca gcgcgtccga ggtaactcgg gcggtctggg aatcatcctc
    10921 gatgagcagc accgatcgct tcggccgttc gcgaaccgat tgccgctcga tggatgcgat
    10981 caccgcgtcc gcggccagct gcacctctcg cggactgcgg aagaagcgag gaatcagccc
    11041 cggcgccgtt cggatgatgg tctgccggga cattgccgtt agcaggaaga catgcgagag
    11101 cgtctccggc tgctccagtt cgatgtagtt cagcactgca aatccgttga gtccgtggcg
    11161 gatcatcggg tcgagcacga tggcgggata gctgttggtt cgcagcttct caattgcggc
    11221 gaggctttcg ccggccgttt cgactgtcaa tccggcgtcc atgaagcggt tgcgaagctc
    11281 cgaaatcgtt tccaggttgt cgttgacgat caacaccacg ttatcgttct tcacacgctc
    11341 tccttcgcat gcagcacttg atggatcgtt cgcagcaact cctccgagga atacggcttc
    11401 atgaggaagg cgacgcgcgg attgttgaga agctcccgga tctcgcgctc gaaagcgtga
    11461 ccgctggaga agatcaccgg cactcgagac tgcgccgcga ttcgttcata gacctttctg
    11521 ccgtcgtcgt ctggtagatt cagatcgagc acgatcacgt caggctggag cttgaacatg
    11581 gccggtacta cgtcggcgcc tttgccgacg acatgagcat cgattccctc ggcttcgagc
    11641 gaccattgca gaccgcaagc gaccgcctcg tcatcctcga cgagcaggac gtggagggtg
    11701 tccgggagca ccgtttcttc gtggggagcg tctggctccg cctgcggtcc cgtttcctcg
    11761 cacgatggga tgtagagatg aaaggtgctg ccgatcccgg gctcgctttc gacagtgata
    11821 tgcccgcggt gcgctgcgac aatctggaat acaaccgata gccccagccc tgtcccgatt
    11881 ttcttcgtcg agaagagcgg ttcgaagatg tgtggaaggt tctcgctggc gatgccttcg
    11941 ccggtatcgg tcaccgtgaa atggatgaac cgctgctgga gctcgcgcgg ttggtcggga
    12001 gacaagccaa gagtcagcgt gcctccgctc gcgagcatcg cgtcacgggc gttcagcgca
    12061 agattgatca ggacctgtga caactgcgcg cggtcggcac tgacggtgac atcttgtggc
    12121 acttcgccgg cgtcgacgct gagagcgatg ctatcgggaa gcatcgggcg gatctcttcg
    12181 gacacctggc ggatcaggtc atcaaccttc accggatgca acgtgagctg cgccgggcgt
    12241 ccgaaacgca ggatgtccgt cgtgatccgc tttccgcggc tgatcgaggc ggcgacaaga
    12301 tcggagaagc ggaggagccg cggattgttc gctccgtaac ggcgaatgac ttcgacgacc
    12361 ggttgaatgc ccatgaggac gttgttgaac tcatgcgcca cctgcgcgga cagccggccg
    12421 aggctggtaa gccggtgcaa ctgctcgagc tcctgttcga gcgcctttcg ctccgtcacg
    12481 tcacgcgtgc tgataaccag tccctgaacc tgggggtgat gcagcaggtt cgtgccgacc
    12541 gattcgagca gacgccacgc tccatcctta cgccggaggc ggtatggctc gcaggagact
    12601 ggattgcgac cagcggcgac gagccgttga aacgacgcaa tgacgcgatc ccgatcgtcc
    12661 ggatggacga gcgcgaagtg gctgcttccg atcaactcct ccgtgctgta acggagaacc
    12721 cgttccgccg aggggctgac gtacgtgatg cggccctcgg tgtccaggat cgcaatgacg
    12781 tcggaaacgt tttctgtcag cgcgcggaag tactcctctc gctccttcac gcgccggttg
    12841 atggcggctt gtccggtcac gtccacggcg gcgccgatga tgatgcggcg gttatcgacg
    12901 tagatcacgg acccgtggat ttccgcctcc aggatgctgc cgtcct
//
LOCUS       DATLVW010000218        10386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_256393, whole genome shotgun sequence.
ACCESSION   DATLVW010000218 DATLVW010000000
VERSION     DATLVW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256393"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VNN25_07035"
     CDS             <1..205
                     /locus_tag="VNN25_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91318.1"
                     /translation="LPHTFASQASFVHDAMTTQRGLGCACYMRIRSKPKPTEDGIDSS
                     DQSISVGFGFDLTPSAKKKKGAV"
     gene            672..1343
                     /locus_tag="VNN25_07040"
     CDS             672..1343
                     /locus_tag="VNN25_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91319.1"
                     /translation="MKRLPAHLAACLILAALPLAAANVTGKVAFVTKRGQNPIPAETL
                     VWLEPLNGRVTPRAPGMFQLVTRNKMLIPHVLAVPVGSTVSFPNDDPISHNLFSLSSN
                     NSFDLGLYRKGAGKSQKFAAPGIVNVYCNVHPNMSAVIHVLSTPYFGFADANGNFSVG
                     DVPPGKYRLDVWNEQGGTSEAPVVDVTSTGPSQAVTLTLDSRNYRATVHMNKVGKPYQ
                     QPRDY"
     gene            1345..4230
                     /locus_tag="VNN25_07045"
     CDS             1345..4230
                     /locus_tag="VNN25_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH91320.1"
                     /translation="MTAEPIAIPTRTVTGELPVRKAPAVEKAKFPLVWKLFGLTALLI
                     VIVVGIAIGITIQRANTIARTTVDKSIASAAKLFKELERQRLGRLSLSAEFLGRDPSF
                     VAYMEHAMSGTVDVAAATPVAGAPAGTKPPVASQTAPPATPAPTAAPSIDFADILDQL
                     NQQKESLHSDLMIVLDGQGRVLSRTDRPALSGEKMEDLYASTPMVKQLVDDASINVVT
                     GVITTGGHLYHAAMAPMSLGTNNVRIGYLINAYAIDEVFANRIAESTNAGVIFAPADV
                     SSPPVRSTDAPIFNMAQMKSVDPILKTGKDVQPSTVEIERSKYVMTGGPLQSVVVADQ
                     KGAPASPPAGAAVFLRSLDIELKPFKDIESALLAGGGGALLLAFIFSWLIAKRLTHPI
                     EELAGIAQAVTGGDYTVHPPIDRSDEVGILGRSFAKMITALRDKAEIEELYEQMAAKS
                     EERESAGTQPQSEAAKLDEGTLLVTDLRELPATVGEGDAANVIAAVSRAMKLQESEVA
                     RQDGFVREIIGHRLVSVFRGDRGILHAIRAARAINEELATMGEGHMTIGAGIATGEFV
                     TGSVELEGDTGIAIVGNAPLLAMLFAWHAPTGYAYISYETAQAAGGEILSSSSAEQVQ
                     LKWLPQPLPVASLPLVSLTTNMMRAIGQASSSMATMRIGETPPGLTMPGIAAQELTPG
                     SLFANRYRIDQIIGRGGMGVVYKAVDTQLDETVAIKTLPGDVMSRSPEDLERFKREIR
                     LARKITHRNVLRTYDYGEAEGVYFISMEFVRGYTLAELLEEAEAHQMATRVAMGIGRQ
                     ICRGLEAAHEQGIIHRDIKPQNVLIDHKGEVKLMDFGIARMAEAHEGMTQAGLIVGTP
                     HYMSPEQVQGKQLDPRSDVYSMGVMLYEMLAGQKPFVSSSLTGVLTAHITETARPPIE
                     VRPEIGRAVNAIVMRCLEKDPKARYATAGELLHDLDSVQMQQRSAAA"
     gene            complement(4497..5030)
                     /locus_tag="VNN25_07050"
     CDS             complement(4497..5030)
                     /locus_tag="VNN25_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91321.1"
                     /translation="MKCTKDTVATLQSVATIIALLVGAWWVLRRRRTYPRANFKHLVS
                     HVPLDDDTYLVRVTIAIENVGDVLLRLEESVGAIQQVVPLHDQLGPALKARVTLGASD
                     QSELAWPFIDTRRRNWSGNEVESGESASFGFELFAPRTVRFVLVQTYFRNITKPADQG
                     WNTSTLYELKLSGTTNL"
     gene            complement(5254..5646)
                     /locus_tag="VNN25_07055"
     CDS             complement(5254..5646)
                     /locus_tag="VNN25_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH91322.1"
                     /translation="MTPQTPDRNPIVPDGWHTVTPRIVATNAEGLVAFIKHVFGATGD
                     YLETRPSELRIGDSMVMVSDSGARDPMPAFLYVYVDDTDATYRLAIDAGARSLEEPLE
                     TPYGDRRSMVEDKWGNTWQIATRKAPSE"
     gene            complement(5720..6454)
                     /locus_tag="VNN25_07060"
     CDS             complement(5720..6454)
                     /locus_tag="VNN25_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91323.1"
                     /translation="MSPPGSVSDAVAYESMHVQPLTEIEITIMSDHNTPSPSTPSADL
                     SPEDRAARMIALIEELESLIPGFRPHDAAEIRRVATAARFGRELIPAMISAVTSFPPF
                     AERGVFDAERAKCMLQSDSALRPVAHRLAALLEGFEFTLESNVADTSTEALQGYAYAK
                     HFARSPAGAGLRPYLAIMRSAVRKSINLRKDATPKPAPPTTPPGDQGFMAPNLASRST
                     APATPDDDLPEDFRKALDSVSDDEEE"
     gene            complement(6952..7623)
                     /locus_tag="VNN25_07065"
     CDS             complement(6952..7623)
                     /locus_tag="VNN25_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91324.1"
                     /translation="MLKRLILLCAVAGSVYAAPPKAYHLEIEANPASPFPYLGKFGTV
                     TLHVYPAGIRAETFWLSGFTRNGASTVTVENPLGRMYTEIPLPQISATLHKLSTSGVE
                     SAAPTGITLMNGNVKGIPAHRHRLSYGPEAWIDVWTTDTIPENPQLRAVIMEFVRGIS
                     PATAATIGSIRGMPLYVELNFRRYKKVPLVRLKSVTWNADGQDDALKTGMFYMRAPLL
                     DSIWK"
     gene            complement(7682..9514)
                     /gene="rpoD"
                     /locus_tag="VNN25_07070"
     CDS             complement(7682..9514)
                     /gene="rpoD"
                     /locus_tag="VNN25_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXH91325.1"
                     /translation="MTTRLRTANLEQETNFDEGVGELSVEEKYAEVKQLIIVGKEKGF
                     LLYDEIYETLPEEITSLPEELDEIYIRFADLGIDVVEDSEKPLPTNSKSIVTIDGTIE
                     EKEMDQREEVQQDIIAASSGIVDKTNDPVRMYLREMGTVKLLDRQGEVDIAKRIEKGE
                     RKVYKSLSQNRIILEEILKIYESAKKDPRVIKEMIDLAMPEDETIAVATDENGEPKMP
                     AATINKIADIMAIFEKVAKHDKKIKDAKTKLIDPKKLSERTLKQTERSIERLTIELAT
                     ELRGVDFTPQTRNRMINKLKDIDNELAQTASMVRKDDAALKREKDKTRRDFYRRRLQK
                     YQEKLRELETFYGTPHDELRRTIREIRAGEEEADQAKQELIVANLRLVVSIAKKYTNR
                     GLQFLDLIQEGNIGLMKAVEKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVH
                     MIETINKLTRTSRALVQELGREPQAEEIADRMDMPVAKVRKIMKIAQEPISLETPIGE
                     EEDSHLGDFIEDRGVISPIDHVIVANLKEQTGKVLRTLTPREEQVLKMRFGVGDGSEH
                     TLEEVGRSFNVTRERIRQIESKALRKLRHPSRSKKLRPFLDSTF"
     gene            complement(9654..>10386)
                     /locus_tag="VNN25_07075"
     CDS             complement(9654..>10386)
                     /locus_tag="VNN25_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HXH91326.1"
                     /translation="DPDSVVQKHGVDRFLEILGSATDIFEFALAEWGTDVAKLTGREK
                     SERVEQFVPLLSAVSDPVVRNDAAQRIADAFRLEFQTIWSKVRGKASTAPERERQPSA
                     RSASAEKTILTAAIQGKLVASDLARLREEYFEEPACKTIFSIMKADLLSGAPIDFQQV
                     ATHLKGEAELNLLSEISLTEDIDDQILQRIDENLRPMERAYLDRRNLEIQQAISEAQR
                     QGDGEQAAQLGMEKVRILRMLNEAK"
BASE COUNT         1970 a         3070 c         3227 g         2119 t
ORIGIN      
        1 attgccgcat acgtttgcat cgcaagcctc ctttgtccac gatgctatga ccacgcagcg
       61 gggactgggt tgtgcctgct atatgcgtat caggtcgaaa ccgaaaccga cggaagatgg
      121 aattgactcc tccgaccagt caatttccgt cggtttcggt ttcgacctga ccccttccgc
      181 caaaaagaaa aagggcgccg tttaacgggc gcccttccgg ggtggagacc agcccttacg
      241 agccaacccc ctgagtttta ctaggacaat ccatcgaaat tagtacctcc tcccccatga
      301 atttccggcg gacccgcaac tagcgccgct gctaatggat cagcagccca cctcgccagc
      361 gtcacggacc ggatcgaccg ctcctactcg ccctgtggtg gaagggagga gcgggatctt
      421 gatgtcctgt cgttaagctt caaagaacga tgaggccatt tgcctcaccc agaccgtata
      481 ctccaaggca aacgcgtagc gcggctgaat cttccagccc atcccacccg taacctgtcg
      541 agcatggata agatgatacg tttttcgtcc gcaatagcaa taccaaattt caggccaact
      601 cccgagtcca cgcacttgct gatgcgccca gaagcgtgtg ctacgatgac ggctttccgg
      661 agcccattga gatgaaacga cttccagccc atctcgctgc ctgtctcata ctcgccgcgc
      721 tacctctggc ggcagccaac gtcacaggaa aagtggcgtt cgtgacgaaa cgcggacaga
      781 acccgatccc cgccgaaaca ctcgtctggc tcgaaccttt gaacggacgc gtcactccgc
      841 gcgctcccgg gatgttccaa ctcgtcacca ggaacaagat gctgattccg cacgtgctgg
      901 cggttcctgt cggctcgacg gtcagttttc ccaacgacga tcccatctcc cacaacctct
      961 tctcgctctc ctcgaacaac tccttcgacc tcgggcttta ccgcaaaggc gcgggcaagt
     1021 cgcagaagtt cgctgcgccc ggtatcgtga acgtctattg caacgtccat ccgaacatgt
     1081 cggcggtgat ccatgtgttg tcgaccccgt actttggctt cgcggatgcg aacggaaact
     1141 tctccgtcgg cgatgttccg ccgggcaagt accgcctcga tgtctggaac gagcagggag
     1201 gcacgagcga agcgccggtg gtcgacgtca ctagcacggg gccgagccag gccgtgacgc
     1261 tcacgctcga cagccggaac taccgcgcca cagtccacat gaacaaggtg ggcaagccgt
     1321 atcagcagcc gagggactac tgaggtgact gcggagccga tcgccatacc gacgcggacc
     1381 gtgaccggcg agttgcccgt tcgcaaggcg cccgcggtcg agaaggcgaa gtttccgctg
     1441 gtctggaagc ttttcggtct gacggcgctc ctcatcgtga tcgttgtcgg aatcgcgatc
     1501 gggatcacga ttcagcgcgc gaacacgatc gcccggacga cggtcgacaa gtcgattgcc
     1561 agcgccgcga aactgttcaa agagttggaa cgccagcgtc tcggccgact ttcgctcagc
     1621 gccgagttcc tcggccgcga tccctcgttc gtggcgtaca tggagcacgc gatgagcggg
     1681 acggtcgatg ttgccgctgc gacgccggtc gccggcgctc cagcgggaac caagcctccc
     1741 gttgcgtcgc agactgcgcc gccagccacg ccagcaccca cggctgcacc gtcgatcgat
     1801 ttcgcggaca tcctcgacca actgaatcaa cagaaagaat cgcttcacag cgatctgatg
     1861 atcgttctcg acggtcaggg gcgcgttctc tcgcggacag atcgtccggc cctctccggc
     1921 gagaagatgg aagacctcta cgcgtcgacg ccgatggtga agcagctcgt cgacgacgcc
     1981 tcgatcaatg tcgtgacggg tgtcatcacg acgggcggtc atctctatca cgcggcgatg
     2041 gcgccgatga gtctcgggac gaacaacgtc cggatcggct atctgatcaa cgcctatgcg
     2101 atcgacgagg tcttcgcgaa ccggatcgcc gagtcgacca atgccggagt catcttcgct
     2161 ccagcggacg tatcgagtcc gccggtccga tcgacggatg cgccgatctt caacatggcc
     2221 cagatgaaga gcgtcgatcc gattctcaag acgggcaagg acgtccagcc ttcgaccgtc
     2281 gaaatcgagc gcagcaagta cgtcatgacc ggcggaccgc tgcaatcggt tgtcgttgcc
     2341 gatcaaaagg gtgcgcctgc cagcccgcca gcgggagcgg ctgtgtttct tcgctcgctc
     2401 gacatcgagc tgaaaccgtt caaagacatc gagagcgctc tcctggccgg cggcggcggc
     2461 gctcttcttc tcgcgttcat cttctcgtgg ctcatcgcaa agcggctcac gcatccgatc
     2521 gaggagctgg ccggcatcgc tcaggcagtg actggtggtg actacaccgt tcatccgccg
     2581 atcgatcgca gcgacgaagt gggcatcctc gggcgttcct tcgcgaagat gatcacggct
     2641 cttcgcgaca aagcggagat cgaagagctg tacgagcaga tggccgccaa atctgaggag
     2701 cgggagtcgg cgggcacaca gccgcaaagc gaagccgcca agctcgacga agggaccctg
     2761 ctggtcacag atctgcgcga gttgccggct accgtcggcg aaggtgatgc tgcaaatgtc
     2821 atcgccgcgg tgagccgggc gatgaaactt caggaatcgg aagtggcgcg gcaggacggt
     2881 ttcgtccgcg agatcatcgg tcaccggctc gtcagcgtct tccgcgggga ccgcggcatc
     2941 ctgcacgcca tccgggccgc gcgcgcgatc aacgaggaac tggccacgat gggcgagggt
     3001 cacatgacca tcggcgccgg catcgctacg ggcgagttcg tgaccggttc cgtcgagctc
     3061 gaaggcgaca ccggcatcgc catcgtcgga aacgcgccgc tgctggcaat gctcttcgcc
     3121 tggcacgcgc cgacgggcta cgcctacatc tcgtacgaga cggcgcaggc tgcaggcggc
     3181 gagatcctct cctcgtcgtc agccgagcag gtgcaactca aatggcttcc acaaccgttg
     3241 ccggtggcat cgctgccgct ggtttcgctg acgacgaaca tgatgcgtgc gatcgggcag
     3301 gcgtccagct cgatggccac gatgcgcatc ggcgagactc ctccgggcct gaccatgcct
     3361 ggaatcgccg cgcaggaatt gacacccggg agcctgttcg ccaaccgata ccgcatcgat
     3421 cagatcatcg ggcgcggcgg gatgggagtc gtctacaagg cggtcgatac ccagctcgac
     3481 gagacggtgg ccatcaagac gctgcccgga gacgtgatgt cgcgttctcc cgaggacctg
     3541 gagcggttca agcgcgagat caggcttgcc cgcaagatca cgcatcggaa cgtcctccgc
     3601 acctacgact acggcgaagc ggaaggcgtc tacttcatct cgatggagtt cgtgcgcggg
     3661 tacacgctgg ccgaacttct ggaagaagcg gaagcgcacc agatggctac gcgcgtggcg
     3721 atgggcatcg gcaggcagat ctgccgtgga ctcgaagcgg cgcacgagca ggggatcatt
     3781 caccgcgaca tcaagccgca gaacgtgctc atcgatcaca agggcgaggt gaagctcatg
     3841 gacttcggca tcgcccggat ggccgaggcg cacgaaggga tgacgcaggc ggggttgatc
     3901 gtgggaacgc cgcactacat gagtccggag caggttcagg gcaaacagct cgatccgcgc
     3961 agcgacgttt actcgatggg cgtcatgctc tacgagatgc tggcaggcca gaagccgttc
     4021 gtctcatcgt ctctgaccgg cgtgttgacg gcccacatca cggagacggc caggcctccc
     4081 atcgaagtcc gccccgagat cggccgcgcc gtgaacgcga tcgtcatgcg ctgcctggag
     4141 aaagatccga aagcacgcta cgccaccgcc ggcgaactgc tccacgatct cgattcggtg
     4201 cagatgcaac agaggtcagc ggcggcgtaa gcgctaacta gttcttcctc gatctcatta
     4261 tcgagaaaga tctcaagcag gagccgatcg agattcctag cgcgccggac gcttcggcgg
     4321 agtgaggggt tgcggggttg cggactcttt aggccgtttg cctgtctctg agaggtcggg
     4381 cggtgatgcc ggctgtacct tttcagccag cagagccgcg attctgggat ctggcggacc
     4441 ttggcgagtg tattcattgg ccatcattgc acctcccgcg agcggacgct acctgctcat
     4501 agattagtcg ttcctgacaa tttaagttcg tagagagtcg acgtattcca gccttgatct
     4561 gcgggcttcg tgatgtttct gaagtaggtc tgcacgagca cgaagcgcac ggtccgtggc
     4621 gcgaatagct cgaacccaaa cgaggccgac tcgcccgact cgacctcgtt cccgctccag
     4681 ttgcgcctcc tcgtgtcaat gaacggccac gcgagctcgg actgatccga agcccccaat
     4741 gtgacccgtg cctttagtgc aggtcctaac tggtcgtgaa ggggcaccac ctgttgaatt
     4801 gcaccaaccg actcttccaa tcgcagaagc acgtcgccga cattctcgat tgctatcgta
     4861 acccgcacga gataggtgtc gtcgtcaagc ggaacatgcg agacgaggtg cttgaagttc
     4921 gcacgtggat acgtacgccg ccggcgaagc acccaccatg cgccaaccag gagcgcgatg
     4981 atcgtagcta ccgactgcaa cgtcgccacc gtgtctttgg tgcacttcaa ccagcctgga
     5041 gaatcggcga tcgccacgct cgcgatcgcg agggaactgg attggtattg gttgtccata
     5101 gcgtcgctga ggatccctcg gagaggcaga acatccgagg aatctgcgac ctgtggagga
     5161 aagtgctacc ggaactcctc ggaatggaaa gtgtcgctac gtgacgtaga gtcagagggt
     5221 ggggactgaa aacaatggtc tggcaccatg gcactactca gatggtgctt tgcgtgtggc
     5281 gatctgccaa gtgttccccc acttgtcttc caccatgctg cgccggtcgc cgtagggagt
     5341 ttcgagtggc tcctcgagcg atcgcgcgcc ggcgtcgatg gcgagccgat acgtcgcgtc
     5401 ggtatcgtcg acatagacgt agaggaacgc gggcatcgga tcgcgtgcac cggagtcgct
     5461 gaccatgacc atcgagtcgc cgatccgcag ctccgaggga cgagtttcga gatagtcacc
     5521 cgtcgcgccg aatacgtgtt tgatgaatgc gacgagacct tccgcgtttg tggccacgat
     5581 tcgcggagta accgtgtgcc agccgtcggg gacgatgggg tttcgatcag gtgtctgtgg
     5641 agtcatgatg cgattcaagt gcggttgaga ggccgcgtca agttggtcgc acagctatgc
     5701 ctccagagaa aatgcggcct tactcctcct cgtcgtcgct aacggagtcg agcgccttgc
     5761 ggaaatcctc ggggaggtcg tcgtcggggg tggcgggggc ggtgctgcgg ctggcgaggt
     5821 tcggggccat gaatccttgg tcgccgggtg gggtggtggg cggggcgggc tttggggtgg
     5881 cgtctttgcg gaggttgatg ctcttgcgga cggcgctcct catgatggcc aggtatggac
     5941 ggagaccggc gccggcgggg ctcctggcga agtgcttggc gtaggcgtag ccctgcagtg
     6001 cctcggtgct cgtgtcggcg acgttgcttt ccagcgtgaa ctcgaagcct tcgaggaggg
     6061 cggcgaggcg gtgggcgacg gggcggagtg cgctgtcgga ttgcagcatg cacttcgcgc
     6121 gctcggcgtc gaacaccccg cgttcggcaa agggggggaa cgaggtcacc gcggagatca
     6181 tcgccggaat cagctccctg ccaaagcggg ccgcggtggc aacgcggcga atctctgcgg
     6241 catcgtgcgg ccgaaagccg gggatgaggc tttcgagctc ttcgatgagg gcgatcatcc
     6301 ttgcggcgcg gtcttcaggg gaaaggtcgg cagacggggt ggagggagag ggggtgttgt
     6361 gatcgctcat gatggtgatc tcaatctccg ttaggggttg aacgtgcatc gactcgtacg
     6421 cgacggcatc ggacacgctg ccgggcgggc tcacatctgt gctccaatca ttgagacgtg
     6481 gcaggcgcac cgaaagttac ggagggtttc ccaaggctcc cgtcgatagg ctagatcgcc
     6541 ctcggagatg cgaagatccg cctcggaact aaatagctcg ccctcggagc taaatagttc
     6601 gcgctcggaa ctaaatagtt cggcctcgga actaaatagc tcgccctcgg aactaattag
     6661 ttcggtctcg gaactcgata gttcgatctc ggaactcgat agttcgacgc gggaactaga
     6721 tagttcgaac ggggaactcg atagttcgga ccagaaacca aatagatcga ccggggaacc
     6781 aaatagttcg atccttggaa ctaattagtt cggacgggga actaattagt tcgactcggg
     6841 aactaattag ttcgattggg gaactattct cttcgatcgc aaactactga ctgagaatca
     6901 gttatcgtcg tttcacgcga aggcgcgaag acgcgaagga acgtctgccc ttcacttcca
     6961 gatcgagtcg aggaggggcg ctctcatgta gaacatgccc gtcttcaggg cgtcgtcctg
     7021 gccgtcggcg ttccaggtca cgctcttcag gcggacgagc ggcacctttt tgtagcggcg
     7081 gaagttcagc tcgacgtaca gcggcatgcc gcggatggag ccgatcgtcg cggcggtggc
     7141 gggggagatt ccgcgcacga actccatgat gaccgcgcgg agctgcgggt tttcggggat
     7201 cgtgtccgtt gtccagacgt cgatccaggc ttcgggtccg tacgagaggc gatggcgatg
     7261 ggcggggatg cccttcacgt taccgttcat cagcgtgatg ccggtgggcg ccgcactctc
     7321 gacgccgctc gtcgacagct tgtgtagtgt ggcgctgatc tggggcagcg gaatctcggt
     7381 gtacatgcgc ccgagggggt tctcgacggt gacggtgctc gctccgttgc gcgtgaagcc
     7441 gctcagccag aacgtctccg cacgaatgcc ggccgggtag acgtggagcg tgaccgttcc
     7501 gaacttgccg aggtagggaa agggcgacgc cggattcgct tcgatctcga ggtggtatgc
     7561 cttcggaggc gcggcgtaga cactaccggc gacggcgcag aggaggatca atcgtttcaa
     7621 catgaacatc aacaaaaagg gcggccgatg aggccgccct tgcattctat ctggatagag
     7681 gtcagaacgt ggagtcgagg aacggacgca gcttcttcga tcgcgacggg tgccgcaact
     7741 tgcggagcgc cttcgattcg atttgccgga tccgctcgcg ggtgacgttg aacgagcggc
     7801 ccacttcttc gagcgtgtgc tcggagccgt cgccgacgcc gaagcgcatc ttgagaacct
     7861 gctcctctcg cggagtgagc gtgcgcagga ctttgccggt ctgttccttg aggttcgcga
     7921 cgatgacgtg atcgatgggg gagatgacgc cgcgatcttc gatgaaatcg ccgagatgcg
     7981 aatcttcctc ttcaccgatc ggcgtttcga gcgagatcgg ctcctgcgcg atcttcatga
     8041 tcttgcgcac cttggctacc ggcatatcca tccgatcggc aatctcctcg gcctgtggct
     8101 cgcggccgag ctcctgcacg agcgcgcgcg atgtgcgcgt gagcttgttg atcgtctcga
     8161 tcatgtgaac cggaatgcgg atcgtgcggg cctggtccgc gatggcgcgg gtgatggcct
     8221 ggcgaatcca ccaggtggca tacgtcgaga atttgtagcc gcggcggtac tcgaacttct
     8281 cgaccgcctt catcaggccg atgttccctt cctgaatgag gtcgaggaac tgcagcccgc
     8341 ggttcgtgta cttcttggcg atcgagacga cgaggcggag gttggcgacg atcagctcct
     8401 gcttggcctg atcggcttct tcttcgccgg cgcggatctc gcgaatcgtg cggcgcagct
     8461 catcgtgagg cgttccgtag aacgtctcga gctcgcgaag cttctcctgg tacttctgca
     8521 gacggcggcg atagaagtcg cggcgcgtct tgtccttctc gcgcttgagg gccgcgtcgt
     8581 ccttgcggac catcgatgcc gtctgtgcca gctcgttatc gatgtccttg agcttgttga
     8641 tcatgcggtt gcgcgtctgc ggcgtgaaat ccaccccgcg caactcggtc gccagctcga
     8701 tggtgagccg ttcgatcgaa cgttcggtct gcttcagggt gcgctccgaa agcttcttcg
     8761 gatcgatcag cttcgtcttc gcatccttga tcttcttgtc gtgcttggcg accttctcga
     8821 agatggccat gatgtcggcg atcttgttga tcgtcgctgc cggcatcttc ggctcgccgt
     8881 tctcgtcggt ggcgacggcg atagtctcgt cttccggcat cgccagatcg atcatctctt
     8941 tgatgacgcg cggatctttc ttggcgctct cgtagatctt caggatctct tcgagaatga
     9001 tgcggttctg ggagagcgat ttgtagacct tccgctcacc cttttcgatg cgcttggcga
     9061 tgtcgacttc gccctgacgg tcgaggagct tgaccgttcc catctcgcgc agatacatgc
     9121 ggacggggtc gttggtcttg tcgacgatgc cgctggaggc cgcgatgatg tcctgctgga
     9181 cctcttcgcg ctgatccatt tccttctctt cgatcgtgcc atcgatggtg acgatggact
     9241 tcgaattggt aggaagcggc ttctcggaat cctcgacgac gtcgatgccg aggtccgcga
     9301 aacggatgta aatctcgtcg agctcttccg ggagcgaggt gatttcctca ggaagagtct
     9361 cgtaaatctc gtcgtacagc aggaagcctt tttctttgcc cacgatgatg agctgcttta
     9421 cttccgcgta cttttcttcg acactcaact cgccgacccc ctcgtcaaaa ttggtctcct
     9481 gttcgagatt cgctgtccgc aggcgtgtcg tcatttcgtt cttcgctcct cagatacaga
     9541 acaacacctt tttggtccga acgagacctt gacgcgacgg tcagaagccg gctgaccacc
     9601 ccgtcaaatc atcgtccgca gaggacacca acgtaatcgg caggtgacga taattatttc
     9661 gcttcgttca acatacggag gatgcgtacc ttctccatgc ccagctgtgc cgcctgttct
     9721 ccatccccct ggcgctgcgc ttcgctaata gcttgttgga tttccagatt gcgacggtcg
     9781 aggtacgccc gctccatggg gcgaaggttt tcatcgattc tttggaggat ctgatcgtcg
     9841 atgtcctcgg tgagagagat ctccgagagg agattgagct cggcctcgcc cttaagatgg
     9901 gtcgcaacct gctgaaaatc aataggggca cccgacagaa ggtccgcttt catgatggaa
     9961 aagatcgttt tgcaagccgg ttcctcgaag tattcctctc gtaaccgggc cagatcagac
    10021 gccacaagtt tcccttggat ggcagcggtc aaaatcgtct tttccgcgct ggcggagcgt
    10081 gcggagggct gccgttcgcg ctccggagcg gttgacgcct tccctcgaac cttggaccag
    10141 atcgtctgga actccagccg gaaggcgtcc gcgatccgct gcgccgcgtc gtttcggacg
    10201 acaggatcgg aaaccgccga cagcagcggc acgaattgct caacccgctc cgatttctcg
    10261 cgtccggtga gtttggcgac gtcggtgccc cattcggcga gtgcgaactc gaaaatgtcg
    10321 gtggcgctgc cgagaatctc caggaaacgg tcaacgccgt gcttctggac gaccgaatcg
    10381 gggtcc
//
LOCUS       DATLVW010000219         6198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_504689, whole genome shotgun sequence.
ACCESSION   DATLVW010000219 DATLVW010000000
VERSION     DATLVW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504689"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VNN25_07080"
     CDS             complement(<1..542)
                     /locus_tag="VNN25_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91327.1"
                     /translation="MNGIRVEGLTKTYHPFGAKRPVLKGVDLTIAPGEVVGLIGPNGA
                     GKTTLMSCIVGFLNADEGTITIDGRSNDDLDVRRRTGFVPERMNFDRRANGGAFLRYM
                     ARLAGVPRDRVHARVDELLARLGLADAEDKRLSQYSRGMLQRIGMAQALMLDPDFLFL
                     DEPTSGLDPNGVFLVRDLIA"
     gene            complement(539..799)
                     /locus_tag="VNN25_07085"
     CDS             complement(539..799)
                     /locus_tag="VNN25_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91328.1"
                     /translation="MPEPQTTPMGRLDAFDVGPKKLTVQTEFFWRPEWRIETKVYLAG
                     SVKKVYTEDLTGTPESDLQRTIDLFHKTKIDEIVAGLRKLQQ"
     gene            complement(821..1660)
                     /locus_tag="VNN25_07090"
     CDS             complement(821..1660)
                     /locus_tag="VNN25_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH91329.1"
                     /translation="MNIFDIHIHIQPWEMLKPEVLSMLSDPSHANAKDILSSADNVVR
                     YLDSERVERICCINYVSPDVMGFTRETNDWIANFTRDHRDRLLPVGSVNPLHEINVRD
                     EIRRVLDLGIRMIKLHPPHQLFSPNAYKDGDLWQLAEIYRECEDRGVPVMFHTGTSIF
                     PRARNVFADPMPIDDVANDFPRLPIILAHAGRPLYGETAFFLARRHPNVMLDISGIPP
                     KALLRYVPRVADLASKIVWGTDWPSPGVVSLRKNVDDFLALGLGEEVERKILWDNASR
                     LFA"
     gene            complement(1768..2217)
                     /gene="arsN2"
                     /locus_tag="VNN25_07095"
     CDS             complement(1768..2217)
                     /gene="arsN2"
                     /locus_tag="VNN25_07095"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HXH91330.1"
                     /translation="MNNGEITISGATAADVPVIEQLLLRNQLPTAGLEDHWKTFIVAR
                     DGGVVVGCAGSEAYQFVALIRSVAVAESHRSRGVGRRIVRQLLDRLSSRGIREFYLLT
                     TTAQDYFAKRGFKPIERDEIHPQLLASAEFQGACPDTAVCMRLVMMA"
     gene            complement(2291..2557)
                     /locus_tag="VNN25_07100"
     CDS             complement(2291..2557)
                     /locus_tag="VNN25_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91331.1"
                     /translation="MDNRDQMSKNKQSDMDRNTSSQSGKGKSDSSADFGQNKGRSDNL
                     NDPNSRGSKSGSYDTSSKSDKNLDSSSGNSKSGQSSGKDIGNRH"
     gene            complement(2627..3499)
                     /locus_tag="VNN25_07105"
     CDS             complement(2627..3499)
                     /locus_tag="VNN25_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXH91332.1"
                     /translation="MNDVLYFNGRFTTTAERVLGVEDRGFQFGDALYEVFKFSGRKAI
                     FLADHYRRLERGLAEVEIKSPWDEGRFFAVMRDLIERTAFDDGIVYIQISRGEAERKH
                     FFPENLEPTVVAYSRAFHFPDAAKKENGIRLITTPDLRWKHCDVKSVNLLASVLAKKK
                     AQRAGADEVLLLDDGVVREGGSSSFFVVRDDEVITHPLDEHILPGVVRDRVIGLAEHA
                     GLRIEQRPLAVDELNEIDEAFITSTTMGVMPVASIDGRAIGARIGAVTLRLQRLLDAE
                     EIADAAVTHSSSAV"
     gene            complement(3597..4796)
                     /locus_tag="VNN25_07110"
     CDS             complement(3597..4796)
                     /locus_tag="VNN25_07110"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXH91333.1"
                     /translation="MDYKRLLTQVERTLEQIENTESTRITIEQIGETIAKNFREELGI
                     TGGRIYELTDENCYELVGRFGVCTDGPLGITVPRDYKPIELVLENGVVVMEPNDPGVD
                     PVLEAQLGALRFAAVAAGDNHTHILGFDVAPHLSREDILFSLNLIRYTVNQRLRADRY
                     QSLIVEAQRIQQSILPQRVPQYKGFDIWGKTVSADVVSGDFYDYIPISDNILGLAIAD
                     GSGHGLPAALVVRDIYMGLRMATDRDFKIIRTMEKLNHIIHRGRLTTKFVSLFYGELE
                     TGGILIYCNAGHNFPFILRADGVTYEMLANGGPVLGPTPDATYMRGFAKMNAGDLLCM
                     YTDGVVEAHNSDDEEFSLERLQTLVAANRERHAKEIGKEILARVTEWGPGEQDDRTVV
                     IVKAMDP"
     gene            complement(4870..5814)
                     /locus_tag="VNN25_07115"
     CDS             complement(4870..5814)
                     /locus_tag="VNN25_07115"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXH91334.1"
                     /translation="MYGPLKIFTGRAHPGLAQEICAILGIHPGHVSLYSFSDGENYVQ
                     IDENVRGADVFVIQPTSPPVNDNLMELLIMLDAFKRSSASRVTAVLPYYGYARQDRKD
                     KPRVPITSKLVADLITAAGADRVLTLDLHASQIQGFFNIPVDHLFAAPVIVRHLRELG
                     MEDLTIVSPDAGGVERARAYAKRLGATLAIIDKRRTGANQTEVMNIIGDVEDRNVVVV
                     DDIIDTGGTLIHSGDALLAKGARSISASCTHAVLSGPAITRINNSQLKQVIATNSMPM
                     ADRQAECPKITTLSIGDLLAQAIKRIHDEDSVSSLFEM"
     gene            5986..>6198
                     /locus_tag="VNN25_07120"
     CDS             5986..>6198
                     /locus_tag="VNN25_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91335.1"
                     /translation="MRGTHGWAEVSRERKREIIDAIVANTPTKGPVHAELDLTDRCNV
                     ACYFCNQQDLRTKEQIPIKKTTELIDE"
BASE COUNT         1169 a         1814 c         1976 g         1239 t
ORIGIN      
        1 tcggcgatca ggtcgcgtac gagaaagacg ccgttgggat cgagtcctga ggtgggctca
       61 tcgaggaaaa gaaaatcagg atcgagcatc agcgcctgag ccatgccgat ccgttgcagc
      121 atgccgcgcg agtattgcga gagacgctta tcctcggcgt cggcaagccc gaggcgcgca
      181 agcaactcat cgacacgcgc gtgtacgcga tcgcgaggca cgcccgcgag ccgcgccatg
      241 tagcgcagga acgcgccgcc gttggcgcgg cggtcgaagt tcatccgctc ggggacgaat
      301 cccgtccggc ggcgaacatc gagatcatcg ttcgaccgtc cgtcgatcgt gatcgtcccc
      361 tcatccgcat tcagaaagcc gacgatgcac gacatgagcg tggttttgcc ggcgccattc
      421 ggtccgatga ggccgaccac ctcgccgggc gcgatcgtca ggtcgacgcc tttgagcacg
      481 ggacgcttcg cgccgaacgg gtgatacgtc ttcgtcaagc cttcgactcg gattccgttc
      541 attgctggag tttgcgcaag ccggccacga tctcatcgat cttcgtcttg tggaagaggt
      601 cgatggttcg ctgcagatcg ctctccggtg tgccggtgag gtcttcggtg tagaccttct
      661 tcaccgagcc ggccagatac accttcgtct cgatgcgcca ctcagggcgc caaaagaatt
      721 cggtctgcac ggtgagtttc tttggcccga cgtcgaacgc atcgaggcgt cccatcggcg
      781 tagtctgcgg ttccggcatg cccacgtatg gtagcagcgc tcaggcgaag aggcgcgacg
      841 cgttgtccca caagatcttg cgctcgacct cttcgccgag tccgagggcg agaaagtcgt
      901 ccacgttctt gcgaagcgag acgacgcccg gcgatggcca gtcggtcccc cacacgatct
      961 tgcttgcgag gtcggcgacg cgcggaacgt accggagaag cgctttcggc gggatgccgg
     1021 agatgtcgag catgacgttg gggtggcgtc gcgccaggaa gaacgcggtc tcaccataca
     1081 gcggccgtcc ggcatgcgcg aggatgattg gcagccgcgg aaagtcgttg gcgacgtcat
     1141 cgatcggcat cggatcggcg aagacgttgc gcgcccgcgg aaagatcgac gtaccggtgt
     1201 gaaacatgac ggggacgccg cggtcctcgc actcgcgata gatctccgcg agttgccaaa
     1261 ggtctccatc cttatacgcg ttcggcgaaa agagctggtg cggtggatgg agcttgatca
     1321 tgcggatgcc gaggtcgaga acgcggcgga tctcatcgcg gacattgatc tcgtgcagcg
     1381 gattgaccga gccaaccggc agcagacgat cgcgatgatc gcgcgtgaag ttcgcgatcc
     1441 agtcgttcgt ctcgcgcgtg aagcccatga cgtcaggcga gacgtagttg atgcagcaga
     1501 tgcgctcgac gcgttcgctg tcgaggtagc gaacgacgtt gtccgccgag gagaggatgt
     1561 ctttcgcgtt ggcgtgcgaa ggatcggaga gcatcgaaag cacttcaggc ttgagcatct
     1621 cccacggttg gatgtggatg tggatatcga aaatgttcat gactcattat gcggcgcgac
     1681 gatcaaagaa accggttcac gcggagccgc ggagatgcgg agttgtatct ccgcgacctc
     1741 cgcgtctccg cgtgaaacgg ttttgaatca cgccatcatc accaagcgca tgcaaactgc
     1801 ggtatccggg catgcaccct gaaactcggc ggaggccaga agctgcggat ggatctcgtc
     1861 gcgctcgatc ggtttgaatc cgcgcttggc gaagtaatct tgcgcggtcg tggtcaggag
     1921 atagaactcg cgaatgccgc gggaggagag ccggtccagc agttgccgca cgatccttct
     1981 tcccactccc cggctgcgat gcgactccgc gaccgcgacc gatcggatca gcgcgacgaa
     2041 ctggtacgcc tcggagccgg cacatccgac gacgacaccc ccatcacgcg cgacgatgaa
     2101 cgtcttccag tgatcctcga gtcccgcggt gggaagctga ttgcggagaa gaagctgttc
     2161 gatgacaggc acgtcggcag cggtagcacc gctgatggtg atctcgccgt tgttcatgta
     2221 cgacgaggat agaacaaaaa ggccgcgcag gtgcgcggcc ctgatggatg tctctgtttg
     2281 taccggcgtg tcagtggcgg ttgccaatgt ccttgccgga gctctgtccg gacttcgaat
     2341 tgccggacga actgtcgagg tttttgtcgc tcttgctcga agtgtcgtag ctgccgctct
     2401 tgctgccacg gctgttcggg tcgttcagat tgtcggagcg tcccttgttc tggccgaaat
     2461 ctgccgatga gtcgctcttg cctttcccgc tctgcgacga ggtattgcgg tccatgtccg
     2521 actgcttgtt cttgctcatc tggtcacggt tgtccatttg tgtctccttt gtttgggctc
     2581 gcttgcccag catgggaagt tgcaatggag atgccgactg atgtcattaa actgctgagc
     2641 tggaatgcgt tacagccgcg tctgcaatct cttccgcatc gagaagacgc tgcaagcgca
     2701 gcgtcacggc accgattctc gcgccgatcg cgcgtccgtc gatcgacgca accggcatga
     2761 ctcccatcgt cgtgctggtg atgaatgctt cgtcgatctc gttcaactcg tcgacagcga
     2821 gcggccgttg ctcgatccga agtccggcat gttccgccag tccgatgacg cggtcgcgga
     2881 cgacgccggg gaggatgtgc tcatcgagag gatgcgtgat cacttcatca tcgcgaacaa
     2941 caaagaagct cgagctgcct ccctcgcgga cgacgccgtc gtcgagcagc aagacctcgt
     3001 cggcgccggc gcgctgggcc ttcttcttcg ccagcacgct cgccagcaga ttgacggatt
     3061 tgacgtcgca atgcttccag cgaagatcag gcgtcgtgat gaggcgaatg ccattctcct
     3121 tcttcgcagc atccggaaaa tgaaaggcgc gtgagtacgc gaccacggtc ggctcgaggt
     3181 tctcgggaaa gaagtgcttg cgctccgctt ccccacgcga gatctgaata tagacgatgc
     3241 catcatcgaa ggcggtgcgc tcgatcaggt cgcgcatgac cgcgaagaag cggccctcgt
     3301 cccacggcga tttgatctcg acctcggcaa gaccgcgctc cagccgccgg tagtggtcgg
     3361 cgaggaagat cgccttccgt ccgctgaact tgaacacttc gtagagcgcg tcgccgaact
     3421 gaaagccgcg atcctcgacg ccgagaacgc gctcggcggt cgtggtgaag cggccattga
     3481 agtagagaac gtcattcatt agtttttttg caacgaaacc gatcaccacg gagacacaga
     3541 gaacacagag gtttctctgt gtcctttgtg tctctgtggt gatcggtttt gagtgattag
     3601 ggatccatcg ctttcacgat caccaccgtc cggtcgtcct gctcccccgg accccactcc
     3661 gtcacgcggg ccagaatctc tttgccgatc tccttggcgt ggcgttcgcg attcgcggcc
     3721 acgagcgtct gcaggcgctc gagactgaac tcctcatcgt cagagttatg cgcttcgact
     3781 acgccatcgg tgtacatgca gagcagatcg ccggcattca tcttggcgaa accgcgcatg
     3841 taagtggcgt cgggcgtcgg cccgagcacc ggaccgccgt tcgcgagcat ctcgtaggtg
     3901 acgccgtcgg cgcggaggat gaacgggaag ttgtggccgg cgttgcagta gatgaggatg
     3961 ccgcccgttt cgagctcgcc ataaaacagc gacacgaact tcgtcgtcag ccgaccgcga
     4021 tggatgatgt ggttgagctt ctccatcgtc cggatgattt tgaaatcgcg gtccgtggcc
     4081 atgcgcagac ccatgtagat gtcgcgcacg acgagtgccg ccggcaagcc gtggccggaa
     4141 ccgtcggcga tggcgaggcc gaggatgttg tcggagatgg ggatgtaatc gtagaagtcg
     4201 ccgctgacga cgtccgcgga aacggtcttc ccccagatgt cgaatccttt gtactgcggc
     4261 acccgctgcg gcaggatcga ttgctggatg cgctgcgcct cgacgatgag cgactgatag
     4321 cggtcagcgc gcagacgctg gttgacggtg tagcgaatga gattgagcga gaagaggatg
     4381 tcctcgcggc tgaggtgcgg cgcgacgtcg aatccgagga tgtgggtgtg attgtcgccg
     4441 gccgcgacgg cggcgaatcg cagcgcgccg agctgcgctt cgagcaccgg atcgacgccg
     4501 gggtcgttgg gctccatgac cacgacgccg ttctcgagca cgagctcgat cggcttgtag
     4561 tcgcgcggca cggtgatccc gagcggaccg tcggtgcata cgccgaaccg tcccacgagc
     4621 tcgtagcagt tctcgtcggt gagctcgtaa atgcgtccgc cggtgatacc gagctcctca
     4681 cggaaattct tggcgatggt ctccccgatc tgctcgatcg tgatgcgggt cgattccgtg
     4741 ttttcgatct gttcgagcgt gcgctcgacc tgggttagga gtcgtttgta gtccatgttc
     4801 gagtgccgcg gggcggcgtc gaagcttatc agacgctatc gcttgacgcg ttcggagcga
     4861 atgcgtgact cacatttcga atagcgagct gaccgaatct tcgtcgtgaa ttcgcttgat
     4921 ggcctgcgcc agaaggtcgc cgatcgacag cgtcgtgatc ttcgggcact ccgcctgacg
     4981 gtcggccatc ggcatgctgt tcgtggcgat gacctgtttg agctgcgagt tgttgatgcg
     5041 cgtgatcgcc ggtccggaaa gcacggcatg cgtgcacgac gccgagatcg agcgtgcccc
     5101 tttcgcgagc aatgcatcgc cggagtggat gagcgttccg ccggtatcga tgatgtcgtc
     5161 gacgaccacg acattgcgat cctcgacgtc gccgatgatg ttcatcacct cggtctgatt
     5221 agctcccgta cgccgtttgt cgatgatggc cagcgtcgcg ccgagccgtt tggcatacgc
     5281 acgagcgcgc tccacgccgc cggcatcggg cgagacgatt gtcaggtctt ccataccgag
     5341 ctcgcgaaga tggcgaacga tgacgggagc ggcgaaaagg tgatcgaccg gaatgttgaa
     5401 aaagccctga atctgcgaag cgtgaagatc gagcgtcagc acgcgatcgg cgcccgctgc
     5461 ggtgatgaga tcggcgacga gcttcgacgt gatcggcacg cgcggcttgt ctttgcggtc
     5521 ctgacgcgcg tagccgtagt acggcagcac ggcggtgacg cggctggccg aggagcgctt
     5581 gaatgcgtcg agcatgatca agagctccat caggttgtcg ttcaccggcg gcgacgtcgg
     5641 ctggatcacg aagacgtcgg caccgcggac gttctcgtcg atctgcacgt agttctcgcc
     5701 gtcggagaag ctgtagagcg acacatgccc cggatgaatc ccgagaatcg cgcaaatttc
     5761 ctgcgcgagc ccggggtggg cgcggccggt gaagatcttg agggggccgt acaagcggcg
     5821 cgattatacc gattcgcatg ccgacttctt tgcgacgttt cacgtaggtc ggactctcca
     5881 gtccgacatc acgtccagtc ggactggaga gtccgaccta cgtaccccgt ctaacagcgc
     5941 aaccggaatg ctccacaacg cgctgccgtt agcggattcg aaatcatgag aggaactcac
     6001 gggtgggcag aagtgtcgcg cgagcgcaag cgcgagatca tcgatgccat cgtcgccaac
     6061 acgccgacga agggccctgt ccacgccgag ctcgacctca cggaccgctg caacgtggcc
     6121 tgctacttct gcaaccagca ggacttgcgg accaaagagc agatccccat caagaagacg
     6181 accgagttga tcgatgag
//
LOCUS       DATLVW010000220         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_177257, whole genome shotgun sequence.
ACCESSION   DATLVW010000220 DATLVW010000000
VERSION     DATLVW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177257"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            725..931
                     /locus_tag="VNN25_07125"
     CDS             725..931
                     /locus_tag="VNN25_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH91336.1"
                     /translation="MKTEKTKKTEPRLISAKAAAQYLGIPYTSLRERVFAGEIPVVKV
                     GRSWYFERVDLDRFVESHKETFNV"
     gene            924..1142
                     /locus_tag="VNN25_07130"
     CDS             924..1142
                     /locus_tag="VNN25_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91337.1"
                     /translation="MFDMNRRSFLFAVVAVPLAPIASARPLFRPFGRPTFRDTVPAML
                     LPGEKVLAPGEQVMTVEHARDLFSKDKE"
     gene            1145..1732
                     /locus_tag="VNN25_07135"
     CDS             1145..1732
                     /locus_tag="VNN25_07135"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXH91338.1"
                     /translation="MPEKHTTSQELAVAPAVSDVAIRRRRCIHLGWDRHAHLALAALN
                     VQTWGDLADLSASTLLLMPRFGRAQLATVQQKLASVGLTLTGARSIGARNAASAFDQV
                     RDRPPVGGVYFLRCEQFVKIGHAADIRERLIDLATITPFDVEIVAYIAQPPGPHARAL
                     EKSLHARFSALRHRAEWFRLEEPLIGFIQEVNDGN"
     gene            1722..1910
                     /locus_tag="VNN25_07140"
     CDS             1722..1910
                     /locus_tag="VNN25_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH91339.1"
                     /translation="MGTESNIHVESVSVREAAVLLRAHEDTVYKLIKSKKLVGFRMGR
                     AIRIHKSAIRAYLRDYAT"
BASE COUNT          416 a          594 c          646 g          384 t
ORIGIN      
        1 aacccgaagt actaccagaa ggcgaaggcg ggcgcggtac tggtgacggc tcacgtcaaa
       61 tccgtgcaat cagccacgaa ccgtatggcg atcgaggtca caaaacagcg gatgaaggaa
      121 ctgcagccgt gatgagcggt gagagagccg accacatgcg acacgtcgtc gcggccagag
      181 gtatatcgag gatatgtaga gtgtgcaaat gctctttcat cgctcggcgg ccaaactcaa
      241 ggacgtgttc agatcgatgc gccacggaat tgtcgcggcg ttcggatgca gaattcagac
      301 gccgtaatcg gagatggcgc aacgagtatc gtcgcttgcg aaaggccagg tatgcagcaa
      361 cgaagagagc gtgggaggta gctcaccgcg acagccagaa cgaacagcgg agagcgaaaa
      421 gagcaaaaaa accaggagca acggcaggcg agaagcgtgc gtatcgtcgg cacaggccta
      481 agcgtctttt gtatcaccgg gaccgatggc tgaaaaagaa aggacttcgg ttggccgagt
      541 tcgtggccgc tcgcgctgat ctgaaggcct tcgtcgctgg tcgactagta acggtccgaa
      601 cggcggccgt catcgctgga tgtagcgagc ggaccgtgtc gaggcgcatc aatgccggtt
      661 tattgagggc gcgtcatttc gggcaccgga tgttagtttg cagattcgat cttgagaata
      721 aatcgtgaag accgagaaga ccaagaagac ggagccgcgc ctgatctcgg cgaaggcggc
      781 ggcgcagtat ctcggcatcc catacaccag cctgcgcgaa cgcgtgttcg cgggcgagat
      841 ccccgtcgtc aaggttggtc gctcgtggta cttcgagcgc gtcgaccttg accggttcgt
      901 cgaatctcac aaggagacgt tcaatgtttg acatgaaccg tcgatccttc ctgttcgccg
      961 tcgtcgccgt gccgctggcg ccgatcgcat cggcgcggcc attgttccga ccgtttgggc
     1021 gtcccacgtt cagagacacc gtgccggcga tgctgttgcc tggtgaaaag gtgctagcac
     1081 ctggagagca ggtgatgaca gtggagcacg cgcgggacct gttctcgaag gataaggagt
     1141 gacgatgccc gagaagcata cgaccagtca ggagctcgcc gtcgcgccgg ccgtgagtga
     1201 tgtcgcgata cgtcgccgcc gctgtattca tctcggttgg gatcgccatg cccatctcgc
     1261 gctggccgcg ctcaacgtgc agacgtgggg agacctggct gatctgtctg cgtcgacgct
     1321 cctccttatg ccacggtttg ggcgggcgca actggcgacg gtacagcaga agctggccag
     1381 tgtggggctc acgctcactg gtgccaggtc catcggcgcg cgcaacgccg cttcggcgtt
     1441 cgaccaggtc agagaccgtc cgccagtcgg cggcgtgtac tttctccgct gcgaacagtt
     1501 cgtcaagatc gggcacgcgg ccgacatccg tgaacgcctg attgacctcg ccacgatcac
     1561 gccgttcgac gtcgaaatcg tcgcctacat cgcgcagccg cctggcccgc acgcgcgcgc
     1621 gctggaaaag agtctccatg cacgattctc tgcgctgagg catcgcgcgg aatggttccg
     1681 gctcgaggag ccgctgatcg gattcattca ggaggtcaac gatgggaact gagtccaata
     1741 tccacgtcga gtcggtcagc gttcgcgagg ccgccgtact cctgcgcgcc catgaggaca
     1801 ccgtctacaa gttgatcaag tcgaagaagc tcgtcgggtt ccggatgggt cgggccatcc
     1861 gcatccacaa gtcagccatt cgcgcttacc tgagggacta cgcgacatag caccgctggt
     1921 tgccgctgat tgccgttcgt gtcggccgtt cctttgtttc ccaccacctc ggccgcattc
     1981 ttgcggctga gcccgcgcct tctcgggcac caacaccagt gacctgatgt cagcgacatg
//
LOCUS       DATLVW010000221         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_256479, whole genome shotgun sequence.
ACCESSION   DATLVW010000221 DATLVW010000000
VERSION     DATLVW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256479"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VNN25_07145"
     CDS             <1..287
                     /locus_tag="VNN25_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91340.1"
                     /translation="PFDISGAPFDTSRAPFDISRAPFNISRASFDISRAPFDISRAPF
                     DISRVPFDISRAPFDHDALSISNQASAISRLKARISSKLPDPQMTQIDAD"
     gene            complement(328..1596)
                     /gene="serS"
                     /locus_tag="VNN25_07150"
     CDS             complement(328..1596)
                     /gene="serS"
                     /locus_tag="VNN25_07150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXH91341.1"
                     /translation="MLSREYLRDHTDDYRTALKNRGATVDIERFLELEAGRRRSIAQV
                     EALKAQRNAATQEIANLKKNRQDATEQIAAMKHAGDEIKQLDERLAGIEADLRSVELF
                     FPNVPHESVPIGPDETHNRVERTWGEKPSFAFTPKAHWDIGEALGILDFDRATKITGA
                     RFSILSGAGAKLNRALMNFMLDVHEQQGYVEVVPPFMVNADSLLGTGQLPKFEEDLFK
                     LTDRDYYLIPTAEVPVTNLHRDEILDAATLPRMYTAYSPCFRSEAGSHGKDTRGLIRQ
                     HQFEKVEIVKFTTPETSWVEHEKLTADAEAVLQALGLHYRVMTLSTGDMSFTAAKTYD
                     IEVWLPGQETYREISSCSNFADFQARRANIKYRTADKKTAFVHTLNGSGLPLGRTLVA
                     ILENYQNEDGSVRVPSALAPYMKGVDVIRS"
     gene            complement(1813..2664)
                     /locus_tag="VNN25_07155"
     CDS             complement(1813..2664)
                     /locus_tag="VNN25_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH91342.1"
                     /translation="MRKPAVILALVSLIAVACVHVRPRELFIAGPAGSIHISDGARGP
                     GLQSALPVLFVHGNGANLTQWQAQLDHVRQSRRAVALDLRGMGKSAVPANGDYSIKAM
                     VSDVAAVADALHLRRFVIVGHSYGGGVVAAYAAAHPDRVAAVVYADSAANVTMPKEAG
                     EKFIAALRKNKDAVVMAWFAPILVNASDAVRDAVLAFVHETPADAMAGALVGLAAHDV
                     GADVRAFKGPRLAIINASLAKSPSSFHSQFPEVPSREMEGVSHWLMMDKPDEFNRILD
                     EVLATVR"
     gene            2707..3129
                     /locus_tag="VNN25_07160"
     CDS             2707..3129
                     /locus_tag="VNN25_07160"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXH91343.1"
                     /translation="MTAKKRILFLCTGNAARSQMAEGLARAFHGDVIEVASAGSRPAG
                     WVHPLAIGALAEIGIDIRDAKSKSAEEFLDQPFDVVVTVCDSAAQDCPLWPGAARIEH
                     WPIEDPTWGEGSGAARFAATRDDLKVRIEELVAGLAGE"
     gene            complement(3786..4115)
                     /locus_tag="VNN25_07165"
     CDS             complement(3786..4115)
                     /locus_tag="VNN25_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HXH91344.1"
                     /translation="MARRSPALNDRKTFTYEEAAALLPEARRITSDAVAAVESLPETE
                     SAATESEQIITDWAGAIIELGIEVKGLWLIDFDCGSGYYCWQHPEPSLQYFHGYEEGF
                     GGRVKLQ"
     gene            complement(4091..>4453)
                     /locus_tag="VNN25_07170"
     CDS             complement(4091..>4453)
                     /locus_tag="VNN25_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91345.1"
                     /translation="TFAVGSPIADGAVLRARTDGVSAWLAKHGDAVKPFVAKLKKAAD
                     DQIEKLDQKHIDDELADLKSKVPEWKAEGADTFGRHLDGVRKQVGKIEDDLRRSNHAE
                     EAKKIKEKFDKWLDEVQR"
BASE COUNT          783 a         1327 c         1449 g          894 t
ORIGIN      
        1 ctccgtttga cattagcggc gctccgttcg acactagccg cgccccgttt gacattagcc
       61 gcgcgccgtt taacattagc cgcgcgtcgt ttgacattag ccgcgcgccg ttcgacatta
      121 gccgggctcc gtttgacatt agccgcgtcc cgttcgacat tagccgcgct ccgttcgatc
      181 acgatgctct gtcgatatcg aaccaggcgt ctgcaatatc aagactgaag gctcgaatat
      241 caagcaagct tccggatccg cagatgacgc agatcgacgc agattgaggc gtcctcatct
      301 gcgtcatctg cggataacct ttggagtcta cgagcgaatg acgtcgacac ccttcatgta
      361 cggcgccagc gcagaaggta cccgcaccga accatcctcg ttctggtaat tctcgaggat
      421 tgccaccagc gtgcggccga gggggaggcc ggagccgttg agagtgtgga cgaaggcggt
      481 cttcttgtcg gcggtgcggt atttgatgtt cgcgcggcgg gcctggaagt ccgcgaagtt
      541 cgagcaggag ctgatctcgc ggtacgtctc ctggccgggc agccacacct cgatgtcgta
      601 cgtcttcgcg gcggtgaagc tcatgtcgcc ggtcgagagg gtcatcacgc ggtagtgcag
      661 cccgagggcc tgcagcaccg cctcggcatc cgcggtcagc ttctcgtgct cgacccagga
      721 agtctccgga gtcgtaaatt tcacgatctc gactttctcg aattgatgct gccggatcag
      781 tccgcgcgtg tctttgccgt gcgatccggc ctcgcttcgg aagcaggggg agtaggccgt
      841 gtacatgcgc ggcagcgtcg ctgcatccag gatctcgtcg cggtgcagat tcgtcactgg
      901 cacctcggcg gtcgggatca ggtagtagtc acggtcggtg agcttgaaga ggtcctcctc
      961 gaacttcggc aactggccgg tgccgaggag ggagtcggcg ttgaccatga agggggggac
     1021 gacttcgacg tagccctgct gctcgtggac gtcgagcatg aagttcatca gcgcacgatt
     1081 cagcttcgcg ccggcgccgg agaggatcga gaaacgcgcg ccggtgatct tcgtggcgcg
     1141 gtcgaagtcg aggatgccga gggcttcgcc gatgtcccaa tgcgctttgg gcgtgaacgc
     1201 gaaggacggc ttctcgcccc atgtgcgctc gacgcggttg tgcgtttcgt cgggaccgat
     1261 cgggacggat tcgtgcggaa cgttggggaa gaagagctcg acgctgcgga gatccgcttc
     1321 gatgccggcg aggcgctcat cgagctgctt gatctcgtcg ccggcgtgtt tcatcgccgc
     1381 gatctgttcc gtcgcgtcct gcctgttctt cttgaggttg gcgatctcct gcgtcgcggc
     1441 gttgcgctgc gccttgagcg cttcgacctg cgcgatggag cgccggcgcc cggcttcgag
     1501 ctcgaggaag cgctcgatgt cgacggtggc gccgcggttc ttgagggcgg ttcggtagtc
     1561 gtctgtatgg tcgcggaggt attcacggga aagcatggcg cgcgattcta gcagcgccgg
     1621 cacgaatcca tttcgcgcga aatcattcgg ggcgccgcgt tagtgcgcct ttctcacaac
     1681 catgaaccac aaagacacag aggtcacaga gttgcactca tgatctctgt gatctcagtg
     1741 cctctgtggt gatcggttct cgcccgattg aaaacgcgcg agcaatttcg ctggaagtgt
     1801 cagaaatgca ggctatcgca ccgtggccag aacctcgtcg aggatgcggt tgaactcgtc
     1861 gggcttgtcc atcatcagcc agtggctcac gccttccatt tcgcgcgacg ggacctcggg
     1921 gaactgcgag tggaacgatg atggactctt cgccagcgat gcgttgatga tcgccaggcg
     1981 ggggcctttg aatgcgcgga catcggcgcc gacgtcgtgc gcggcaaggc cgacgagtgc
     2041 gccggccatg gcatcggccg gggtctcgtg gacgaaggcg agaacggcgt cgcgaacggc
     2101 gtcgctggca ttgacgagga tcggcgcgaa ccacgccatc acgacggcgt ccttgttctt
     2161 gcgcagcgcg gcgatgaact tctcgcccgc ttccttcggc atcgtcacgt tggcggccga
     2221 gtcggcgtag accacggcgg ccacgcgatc gggatgcgcg gcggcgtacg cggccacgac
     2281 tcccccgcca tagctgtggc cgacgatgac gaagcgccgg aggtggagcg catcggccac
     2341 ggcggcgaca tcggacacca tcgccttgat cgagtaatcg ccattcgcag gcacggctga
     2401 cttgcccatc ccgcgcagat cgagcgcgac ggcgcggcgc gattggcgca cgtgatcgag
     2461 ctgcgcctgc cactgcgtga ggttcgcgcc gttgccgtga acgaaaagga ccgggagcgc
     2521 cgactggaga cccggtccgc gggcgccatc gctgatgtga atcgatcccg ccggtccggc
     2581 gatgaacaac tccctcggac gcacgtgcac gcacgcgaca gcgatcagcg acacgagagc
     2641 gagaatgacg gccggcttgc gcatcgccgc cgagaatacc agcgcgagcg tcatacaatc
     2701 ggtgacatga cggcgaagaa acgaattctt tttctctgca cgggaaacgc ggcgcggagt
     2761 cagatggccg aggggctggc gagggcgttt catggggacg tgatcgaggt ggcgtcggca
     2821 ggatcgcggc ccgcgggatg ggttcatccg ctggcaatcg gcgcgctcgc ggaaatcgga
     2881 atcgatatcc gcgacgcgaa gtcgaaatcg gcggaagagt ttctcgatca acccttcgac
     2941 gtcgtcgtca ccgtctgcga ctccgcagcc caggactgcc cgctgtggcc cggcgcggcc
     3001 cgaatcgagc actggccgat cgaagacccg acgtggggcg agggcagcgg cgctgcgaga
     3061 tttgccgcga cgcgggatga tctaaaggtg cggatcgagg agttggtggc cggtttggcg
     3121 ggtgaatgag tcgagattgg tgtcatcctg agaccgccgg agccgcccgc gcgaatcgcg
     3181 atgtttgcgc agtactagcg cggcggcgga ggacgtcgaa ggatctcaaa attcgcagat
     3241 cgtgttcgtc tagcgcgagc gatcgtgacg ctcgaacgag atcttcgtat tttgagatcc
     3301 ttcgaccgtc ctacgccgcc gatgcctgcc ggttgctagt gcagtgaatc ttgcggcggc
     3361 ttcggcggct caggatgaca caacacgttt tgcgcgtatc ggtcaacggc ttcggcggtt
     3421 caggatgaga gcaatcgcgt tctccaatcg ccatgctcgt gatgatcgtc caatcgagat
     3481 tggtgtcatc ctgagactgc cggagccgcc cgcgcgaatc gcgatgtttg cgcagtacta
     3541 gcgcggcggc ggaggacgtc gaaggatctc aaacttcgca gatcgtgttc gtccagcgcg
     3601 agcgaacgtg gtcaagacgc tcaaacggga tcttcgcatt ttgagatcct tcgaccgtcc
     3661 tacgccgccg atgcctgccg gttgctaatg cagtgaatct tgcggcggct tcggcggctc
     3721 aggatgacac aacacgtttt gcgcggtatg gaaaacgggc taaaaaacca atgacccatc
     3781 accacctact gcagtttcac cctcccaccg aacccttcct cgtagccgtg gaagtattgg
     3841 agtgatggtt cggggtgctg ccagcagtag tagccgctgc cgcaatcgaa gtcgatgagc
     3901 caaaggccct tcacttcgat gccgagctcg atgatggcgc cggcccagtc ggtaatgatc
     3961 tgttcggatt cggtggctgc cgattcggtt tccggaagcg actcgaccgc tgctacggca
     4021 tccgaggtga tgcggcgcgc ttcgggaagc agggcggccg cctcctcgta tgtgaaggtc
     4081 ttccggtcgt tcagcgctgg acttcgtcga gccatttgtc gaatttctcc ttgatcttct
     4141 tcgcttcctc agcgtgattc gaacggcgca ggtcgtcctc gatcttcccg acctgcttgc
     4201 gaacgccgtc gaggtgacga ccgaacgtgt ccgcaccttc tgctttccac tccggaacct
     4261 tcgacttcag gtccgccagc tcatcgtcga tatgcttctg atcgagcttt tcgatctggt
     4321 cgtcggcagc cttcttaagc ttcgcgacga acggcttcac cgcgtcgcca tgcttggcca
     4381 gccaggcgct cacgccatcg gttcgcgcgc gaagcacggc gccgtcggcg atcggagaac
     4441 cgactgcgaa cgt
//
LOCUS       DATLVW010000222         5179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_587241, whole genome shotgun sequence.
ACCESSION   DATLVW010000222 DATLVW010000000
VERSION     DATLVW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587241"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3143
                     /locus_tag="VNN25_07175"
     CDS             <1..3143
                     /locus_tag="VNN25_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH91346.1"
                     /translation="CFVLFGVFLLLVPGVTFAIWWRALIAAMFASALTIATFQWWLRT
                     VFQRQNSSIALLNRITAGDLSLAAREIQLATQSARMSAAMRALVSNLERTIRRFAQLA
                     TDVALASGQISGRSRVLARGAAEQLVSTETTSVSVGQIDQSINNVRTSMEDLSANAEE
                     TSTSILEMSASIEEVSRIADTLAEFVEQTSSAFEEMSASINQVATNTESFSSFAIQTA
                     SSMVEMNATTEEIGKSAKQSAELARFVKEAANEGRVAVSGTVEGMRKIQVAVEEAKVV
                     LGELGNRSQEIGEIVRVIDEIAGQTNLLALNAAIIAAQAGERGKGFAVVADEIRDLSE
                     RTSVSTDEIRTLIQNVQRAVERAAEQMTISSDRVSDGVGLTARAEQTLDKILEITARS
                     TNSIGEIARATEEQSRGSAAATAAIEEVTKMVQQTASATQQQSQTARKVGEQTSMVRD
                     YTKHLKRAMSEQETGSRAISRAMENIMGLVQTVLESTSVLATESSAIVKAMGVIQQAT
                     RESNVSIGDLNQMANSLSHESTLLNSELGRFKLPTPTDGGKLVTSTVLWQRLTLDPIN
                     ISAAALGYMSKAIHAHLVMYGEGAELVPGLAERWEVMEQGHVYRFHLRRGVRFHNGRT
                     VEAKDVHDSLVRLLLPELNSASSWLMREVRGAADVIEGRTRTLSGIQVRDQYTVDIIL
                     DEPLAFFLSLLTMHEAAIIASEAARDPEQFRLQPVGAGPFRLVEAIEGDRVRMQRNRD
                     YYIPGIPHLDELEFRLDLRSFRDVAQAFLRGEIDVAHGVPLSIVNDLRADPSIAPYML
                     TTIQLHTSYFGYDCSAPPFNRVEVRQAVNHAINRDRINERVFAGLGVVAKSLLPPGLL
                     GYDETIQGIEYDPERARSLMRQAGYAGGLRVEYRTWDTDEFNNSGQLPLIIEDLAAIG
                     IDVNVTSHSAVDARKQLLKPGHGTVYCANWYADFPDSDNFFYVFFHSDAHSARGIYYS
                     NATVDAQIIEARRSNDPERRAEIYRQLNQMVVREAPLATLFHERFFVLQKPEVRGLHT
                     SLVPPPVRYHETWIEG"
     gene            complement(3252..4175)
                     /locus_tag="VNN25_07180"
     CDS             complement(3252..4175)
                     /locus_tag="VNN25_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXH91347.1"
                     /translation="MQKHPGPLLLHGAMAGVSVLFSLNYIISKIGLHAFNPLTFAWLR
                     IAASALIMNVLLRERNAAPLAPGDGWRVAGFAILGVVINQTLFLAGLSFTSAHVASIL
                     ITTVPVFALGAAIALGRERATAARVGGIALAAAGALLVVGGEGFAGTTKSLVGDLLII
                     GNSLAYALYLVASKPTMARLSARRVIARMFAVSTVVMLPIAAWPMMHESWSAIPPRAW
                     LALALVIAGPTVAAYLMNAWALKYADSSVVAAYTYLQPVFTVTLAAIFLHEQIRPIVL
                     AAAAMIFAGVYISGRPAPPAAREMAVPGNPD"
     gene            4255..4869
                     /locus_tag="VNN25_07185"
     CDS             4255..4869
                     /locus_tag="VNN25_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH91348.1"
                     /translation="MNPISESAATDSELIARTLGGDGNAYGILVDRFQRKIYRVAYAI
                     VRDDVEADTITQDTFVQAYTHLARFQGRAELETWLTRIAINRSRDWLRRRKFVSLFSH
                     DDESDERQYVIEPVDERPDAERQLMAHQLRAAITRAERLLSSQQKIIFRLRHYEHHSL
                     EEIEELLGLQSGTVRAHLFRAIHKVRRELAEWVTMREVNPDETL"
     gene            4856..>5179
                     /locus_tag="VNN25_07190"
     CDS             4856..>5179
                     /locus_tag="VNN25_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91349.1"
                     /translation="MKHFSEAELLETYYTQPGESMPVMMHLSNCHECAARYERLEQKL
                     RQAASCNTEKPATFWTRQRLQIMRRVNAQQVNAATAARTLRVAAAAILAFFLGGALVY
                     ETVRPA"
BASE COUNT         1010 a         1657 c         1673 g          839 t
ORIGIN      
        1 cctgtttcgt cctgttcggc gttttcctcc tcctcgtgcc gggggtgacg ttcgcaatct
       61 ggtggcgcgc tctcatcgcg gcgatgttcg cctccgcgtt gactatcgcc acattccagt
      121 ggtggctgcg taccgttttc cagcgccaaa actcatcgat cgcgttgttg aaccgcatta
      181 ccgccggcga cctctcgctc gccgcgcgcg agattcagct ggccacgcaa tcggcgcgga
      241 tgtcggcagc gatgcgcgcg ctggtctcca atctcgagcg gacgatccgc cgctttgcac
      301 aactggcgac cgacgtggct ctggccagcg gtcagatcag cggacgttcg cgcgtccttg
      361 cccgcggcgc ggccgagcag cttgtctcga cggaaacgac ctcggtctct gtcggccaga
      421 tcgatcagtc gatcaacaac gtgcggacgt cgatggaaga tctctctgcg aatgccgagg
      481 agacgtcgac gtcgattctg gaaatgtcgg catcgatcga agaagtgagc cgcatcgccg
      541 acacgctcgc cgagttcgtc gagcaaacct cgtcggcatt cgaggagatg agcgcgtcga
      601 tcaatcaggt ggcgacgaac accgaaagct tctcctcgtt tgcgattcaa accgccagct
      661 cgatggtgga gatgaatgcg acgaccgagg agatcggcaa gtcggcgaag cagtcggccg
      721 agctcgcgcg cttcgtgaag gaagcggcga acgaaggacg cgtggcggtc agcgggaccg
      781 tcgaagggat gcgcaagatc caggtcgccg tcgaggaagc gaaggtcgtc ctcggcgagc
      841 ttggcaaccg ctcgcaggag atcggcgaga tcgtgcgcgt gatcgacgag atcgcgggac
      901 agacgaacct gctggcgctg aacgcggcaa tcatcgccgc gcaggccggc gagcgcggca
      961 aaggattcgc ggtcgtcgcc gacgagattc gcgatctctc cgagcgcaca tcggtatcga
     1021 cggacgagat ccgcacattg attcagaacg tgcagcgcgc cgtggagcgc gccgccgaac
     1081 agatgacgat cagcagcgac cgcgtgtcgg acggtgtcgg actgaccgcg cgcgccgaac
     1141 agacgctcga caagatcctg gagatcacgg cccgctcgac caactcgatc ggcgagatcg
     1201 cgcgcgccac ggaagagcag tcgcgcggca gtgcggcggc gacggcggcc atcgaagaag
     1261 tgaccaagat ggtgcagcag acggccagcg cgacgcagca gcagtcgcag accgcgcgca
     1321 aggtgggcga gcagacgtcg atggtgcgcg actacacgaa gcacctcaag cgcgccatgt
     1381 ccgagcagga aaccggaagc cgcgccatca gccgggccat ggaaaacatc atgggcctcg
     1441 tgcagacggt gctggaatcg acatcggtgc tggcgacgga gagctccgcg atcgtcaaag
     1501 cgatgggcgt gatccagcag gctacgcgcg agtcgaacgt ttcgatcggc gatctgaatc
     1561 agatggccaa ttcgctctcg cacgaatcga cgttgctgaa ctcggagctg gggcgtttca
     1621 agctgccgac gccgaccgat ggcggcaagc tggtgacgtc gaccgtgttg tggcaacgat
     1681 tgacgctcga tccgatcaac atcagcgccg ccgcattggg ctacatgtcg aaagcgattc
     1741 acgcacacct tgtcatgtac ggcgaagggg cggagctggt gccgggtctg gccgagcgct
     1801 gggaagtgat ggaacaaggt cacgtctacc gctttcacct gcgccgcggc gtccgtttcc
     1861 acaacggtcg cacggtggaa gcaaaagatg tccacgattc gctcgtacgc ctgctgctcc
     1921 cggagctcaa ctcggcgtcg agctggctca tgcgggaagt gcgcggcgcg gcggacgtca
     1981 tcgaagggcg cacgcgaacg ctgagcggca ttcaggttcg cgatcaatac acggtcgaca
     2041 tcatcctcga cgagccgctg gcgttcttcc tttccctcct gacgatgcac gaagcggcca
     2101 tcatcgcgag cgaggcggcg cgcgatccgg aacagtttcg cctgcagccg gtgggcgccg
     2161 gtccgttccg tctggtggaa gcgatcgagg gcgatcgggt gcgcatgcag cgcaaccgcg
     2221 actactacat cccgggaatc ccgcatctcg acgagctcga gttccggctc gacctgcgca
     2281 gtttccgcga cgtcgcccag gccttcctgc gcggtgagat cgacgtggcg cacggcgtgc
     2341 cattgagcat cgtcaacgac ctgcgcgccg atcccagcat cgcgccctac atgctgacga
     2401 cgatccagct gcacacgtcg tatttcggct acgactgctc ggcgccgccg ttcaaccgcg
     2461 tcgaggttcg ccaggcggtc aatcacgcca tcaaccgcga ccgcatcaac gagcgcgtct
     2521 tcgccgggct cggcgtcgtc gccaagtccc tgctcccccc cggtctcctc gggtacgacg
     2581 agaccatcca gggcatcgaa tacgatcctg agcgcgcccg ctcgctgatg cggcaggccg
     2641 gatacgccgg cggactgcgc gtcgagtacc ggacgtggga caccgacgag ttcaacaact
     2701 ccggccaact gccgctgatc atcgaagacc tcgccgccat cggcatcgac gtgaacgtga
     2761 cgtcgcactc ggcggtcgac gcgcgcaaac agctgctgaa acccggccac gggacggtgt
     2821 actgcgccaa ctggtacgcc gactttcccg actcggacaa cttcttctac gttttctttc
     2881 acagcgacgc acattccgca cgcggcatct actacagcaa cgccaccgtc gacgcccaga
     2941 tcatcgaagc gcgacgatcg aacgatcccg agcgccgcgc cgagatctac cgccagctca
     3001 atcagatggt cgtccgcgaa gcgccgctgg ccacgctctt ccacgaacgt ttcttcgtcc
     3061 tgcagaagcc ggaagtgcgc ggcctgcaca cgtcactggt cccgccgccc gtgcggtatc
     3121 acgagacgtg gatcgagggg taacggcgcg gcgatttggt tggacaccag atcaccacag
     3181 aggcacagag agcacagagt gtcttctctg tgacctcagt gcctctgtgg ttcagctgcg
     3241 cccgttgcag atcaatccgg gttccccggc actgccattt cgcgcgcggc gggcggtgcg
     3301 gggcggccgg aaatgtatac gccggcgaag atcatcgctg cggcggcgag gacgatcggt
     3361 cggatctgtt cgtgcagaaa gatcgccgcg agagtcacgg tgaagactgg ctgcaggtag
     3421 gtatacgcgg ccaccaccga gctgtcggcg tacttcagcg cccaggcgtt catcaggtag
     3481 gcggcgacgg tcggtccggc gatgacgagg gcgagcgcca gccatgcgcg cggcgggatc
     3541 gctgaccacg actcgtgcat cattggccac gccgcgatcg gcagcatgac caccgtgctg
     3601 acggcgaaca ttcgggcgat gacgcgcctt gctgataggc gcgccatcgt cggcttggag
     3661 gcgacgagat agagcgcgta ggccagcgag ttgccgatga tgagcaagtc gcccacgagc
     3721 gacttcgtcg tccccgcgaa cccctcaccg cctacgacga gcagcgcccc agccgcggcc
     3781 agtgcaatac cgcccaccct cgccgccgtg gcgcgctcgc gcccgagggc gatggccgca
     3841 ccgagcgcga acaccgggac ggtcgtgatg aggattgagg caacgtgtgc cgaggtgaag
     3901 gagagaccgg cgagaaagag cgtctggttg atcacgacgc cgaggatcgc gaagcctgcg
     3961 acgcgccatc cgtctcccgg cgccagaggt gcggcgttgc gctcgcggag caggacattc
     4021 atgatcaggg ctgaggcggc gatccgcagc caggcgaacg tgagcgggtt gaacgcgtgc
     4081 agcccgatct tcgaaatgat gtagttgagg gagaacagca cggagacgcc agccatggcg
     4141 ccgtgcagaa ggagcggacc gggatgcttt tgcaagggcc ggccatcata atcgcgtaaa
     4201 cagacgagcc aaacggccgt ccaaaggctc gtgggaaccg tagcggcatt tccagtgaat
     4261 ccgatcagcg aatccgcagc gacagacagc gagctcatcg cccgtacgct cggcggcgat
     4321 ggcaacgcct acggaatcct cgtcgaccgg ttccaacgca agatctaccg cgtggcctac
     4381 gccatcgtcc gcgacgacgt cgaggccgac acgatcacgc aggacacctt cgtccaggcc
     4441 tacacgcacc tggcgcggtt tcagggacgg gcggagctgg agacgtggct gacgcgcatc
     4501 gccatcaacc ggtcgcgcga ctggctgcgg cgccgcaagt tcgtcagcct tttttcgcat
     4561 gacgacgaga gcgacgagcg gcaatacgtc atcgagccgg tcgacgaacg gcccgatgcc
     4621 gagcgccagt tgatggccca ccagctccgg gcggccatca cccgcgccga gcggttgttg
     4681 tcatcccagc agaaaatcat cttccgtttg aggcattatg agcaccattc actcgaggag
     4741 attgaagagc tcctcgggtt acagtccgga acggtccggg cgcatttgtt ccgtgccatc
     4801 cacaaagtgc gcagggagct cgccgagtgg gtgacgatga gagaggtgaa tcccgatgaa
     4861 acactttagc gaagcagaac ttctggagac ctactacacg cagcccggcg aatcgatgcc
     4921 ggtgatgatg catctgtcga attgccatga gtgcgcggcg cgctatgagc gcctcgaaca
     4981 gaagctgcgt caggccgcct cctgcaacac cgagaaacca gctacgttct ggacacggca
     5041 acggctgcag atcatgcgcc gcgtcaacgc gcaacaggtg aatgcagcca ccgcggcccg
     5101 cacgctccgc gtcgctgccg cggcgatcct cgccttcttc ctcggcggcg ccctcgtgta
     5161 cgagacggtt cggccagcg
//
LOCUS       DATLVW010000223         4976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_297422, whole genome shotgun sequence.
ACCESSION   DATLVW010000223 DATLVW010000000
VERSION     DATLVW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297422"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VNN25_07195"
     CDS             <1..639
                     /locus_tag="VNN25_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HXH91350.1"
                     /translation="DRGVFIRSMATRGFLGGVARATNDVVDLLDAAGFDIVLVETVGV
                     GQDEVEIIRTVQTNVVVLVPGMGDDTQAIKAGIMEIGDVFVVNKADRPGADRTVTEIT
                     MMMSLVDEHGDWIPPIVKTVASTNQGIEDLDAAIVTHFEHLKASGELDRRNRERVRIR
                     IETMLKEKFMDRLIGDTIPRADYEQLLDDVLRKHNNPHDAAEGVLKRVRFGK"
     gene            636..875
                     /locus_tag="VNN25_07200"
     CDS             636..875
                     /locus_tag="VNN25_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91351.1"
                     /translation="MSLLLGSLALIGVLVLIVLLGAGAIAMIARSRGKHGTSGSLSSA
                     ALEIQSLLEPEKKRVVETMRVASEAGDEDAINGKK"
     gene            939..3206
                     /gene="pheT"
                     /locus_tag="VNN25_07205"
     CDS             939..3206
                     /gene="pheT"
                     /locus_tag="VNN25_07205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXH91352.1"
                     /translation="MKFSRQWLNDYVDLSDLSDDEIGTRLTEIGHAIEAVEKHGDDTV
                     FDLEITTNRVDAMSHLGMARELAAAIGREANVGRTLQSDSERGSRTNVGRTLQSDSER
                     GSRTRESDLRRESDLRQAGQGAEAVTVRIEAPDMCARYTAKVIRNITVKPSSPEIQRR
                     LEEVGLRPINNIVDATNYVMVALGHPLHAFDLDRLIGNTIIVRRGKAGEAMKSLDGEM
                     RKIDPDTVVIADAQRAVGLGGIIGGFESEITQSTRNVLLECAWFEPSAIRRTARRLGL
                     KTDASYRFERRVDPNDTLAVIEEAARLIVESGGGIAEAPIDAVAREVQPKTIRLRTER
                     LHEASANSVGAGYALDLFRRLGFSAEQSHDGLLVNVPTYRGDIFEEADLIEEVLRFFG
                     LNNVPASLPRVTTGDVRREATDVAEDAIRDVLVGCGLSEAVNYSFIRPEWNALITDET
                     PIAITNALSENIEAMRLSLIPGLLETVVFNRSYGTRDGALFEVGRTYHRRGVGSGVWG
                     VEEKTNTSTPTSDAASVARNKTTTNASNTFSHTPPPTPHTPVKEHHRVAVVMYGSIGT
                     FWGDAKRTVDFFDIKGIVEQIAKKLHVDVTFAASDQPWLRGGKRAIAWHGDRQIASLG
                     FLSAELLQKFGIKGDVAAAEIDIEALIASSNTEWKMAPVARFPGVPMILALTHGRDLE
                     YQRIIDTIHSFNVPHLHEVGLRDRFVPEGDDVIKTTLGMWYQAFDRSLTQEEIAGFQQ
                     QLATRLAATLPVKLL"
     gene            3218..3514
                     /gene="zapB"
                     /locus_tag="VNN25_07210"
     CDS             3218..3514
                     /gene="zapB"
                     /locus_tag="VNN25_07210"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HXH91353.1"
                     /translation="MKKKSDDQLSIAGTEEAEILGRLNDRVEKAITTIQALRKERDTL
                     RRELDEANARIQEQGDSAERASTLEEDNERFKRERSEIRDRIESILGNLEALEE"
     gene            complement(3800..4414)
                     /gene="pgsA"
                     /locus_tag="VNN25_07215"
     CDS             complement(3800..4414)
                     /gene="pgsA"
                     /locus_tag="VNN25_07215"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXH91354.1"
                     /translation="MQPIEAAPPLAERAKPTVVPPPDDVAAEIWNLPNSLTLLRIFLV
                     PFLVVVLLTKYSNYLGLAIFGVAAITDYFDGYFARKYNKMTRLGALLDPIADKLLMSA
                     AFISLIELGLARAWMIVIIIGREFAVSGLRSIAAQHGVTIAASPLGKTKMVAQVVAIA
                     LLIIGYELGEFRFIGELALWVVMLFALVSGVDYFIKFSRGVLRG"
     gene            4662..4748
                     /locus_tag="VNN25_07220"
     tRNA            4662..4748
                     /locus_tag="VNN25_07220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4696..4698,aa:Leu,seq:cag)
BASE COUNT         1055 a         1595 c         1482 g          844 t
ORIGIN      
        1 gatcgcggcg ttttcatccg atcgatggcc acgcgcggct ttctcggcgg cgtggctcgc
       61 gcgacgaacg atgtcgtcga cctcctcgat gctgccggct tcgacatcgt cctcgtcgaa
      121 acggtcggcg tcgggcagga cgaagtcgag atcatccgca ccgtgcaaac caacgtcgtc
      181 gtcctggtgc ccggcatggg cgacgacacc caggccatca aagccggcat catggagatc
      241 ggcgatgtct tcgtggtcaa caaagccgac cgtcccggcg cggaccgcac cgtcaccgag
      301 atcacgatga tgatgtcgct ggtcgacgag catggcgact ggattccgcc gatcgtgaaa
      361 acggtagctt cgacgaacca gggaatcgag gatctcgacg ccgcgatcgt cacgcacttc
      421 gaacatctga aagcttctgg cgagctcgat cgccgcaatc gcgagcgggt tcgtatccgc
      481 atcgagacca tgctcaagga aaagttcatg gacaggctga tcggcgacac gattccacgc
      541 gccgattacg agcagctcct cgacgacgtc ctgcggaagc acaacaaccc gcatgacgca
      601 gccgagggcg ttctcaaacg cgttcgattc ggcaaatgag ccttcttctc ggcagcctcg
      661 cgctgattgg cgtcctcgtt ctcatcgtgc tgctcggtgc cggcgcgatc gcgatgatcg
      721 cgcgcagccg cgggaaacat ggaacatccg gttcgctcag ctccgccgcg ctcgagatcc
      781 aatcgctcct cgaaccggag aagaaacgtg tcgtcgagac catgcgcgtc gcctcggaag
      841 ccggggacga agatgcgatc aacggcaaga aataacgctt cgccgacgat tccaatcagc
      901 gcaatcggcc gttcccgtcg gttacaatcc cgcccccgat gaagttctcc cgccaatggc
      961 tcaacgatta cgtcgatctc tccgatctca gcgatgacga aataggcacg cgcctgaccg
     1021 aaatcggcca cgccatcgaa gccgtcgaga aacacggcga cgacaccgtc ttcgatctcg
     1081 agatcaccac caaccgcgtc gacgccatgt cccacctcgg catggccaga gagctcgccg
     1141 ccgcgatcgg aagagaagcg aacgtaggtc ggactctcca gtccgactcg gaacgtggca
     1201 gtcggactaa cgtaggtcgg acactccagt ccgactcgga acgtggcagt cggactagag
     1261 agtccgacct acgtcgagag tccgacctac gtcaagccgg gcagggagcc gaagctgtca
     1321 ccgtccggat cgaagcgcca gacatgtgcg cccgctacac cgccaaagtc atccgcaaca
     1381 tcaccgtcaa gccgtcatcg cccgaaatcc aacgccgcct cgaagaagtc ggcctccgcc
     1441 ccatcaacaa catcgtcgac gccaccaact acgtgatggt cgccctcggc catccgcttc
     1501 acgcttttga tctcgatcga ttgatcggca acaccatcat cgtgcgccgc ggcaaggccg
     1561 gcgaagcgat gaagagtctt gacggcgaga tgcgcaagat cgatcccgac accgtcgtca
     1621 tcgccgatgc acagcgtgct gtcggcctcg gcggcatcat cggcggcttc gaatccgaga
     1681 tcacgcaatc gacgcgcaac gtgctgctcg aatgcgcctg gttcgagccc tccgcgattc
     1741 gccgcaccgc gcgccgcctc gggctcaaga ccgatgcgtc gtaccgcttc gagcgccgtg
     1801 tcgatccgaa cgacacactc gccgtgatcg aagaagcagc gcgtttgatc gtcgagagcg
     1861 gtggtggaat cgccgaggcg ccgatcgatg ctgttgcgcg cgaggtgcag ccgaagacga
     1921 tccgccttcg caccgaacgc cttcacgaag catcggccaa ctccgtcggc gcaggctacg
     1981 cgctcgacct cttccgacga ctcggatttt ctgccgagca gtcgcatgac ggactcctcg
     2041 tcaacgtccc cacctatcgc ggcgacatct tcgaagaggc agacctcatc gaagaagtgc
     2101 tccgcttctt cggcctgaac aacgttcccg cttcactgcc gcgcgtgacg accggcgatg
     2161 ttcgccgcga ggcgaccgat gtggcagagg acgcgattcg cgatgtgttg gttggctgcg
     2221 gcctttcgga agcggtcaac tactcgttca tccgccccga atggaatgcg ctcatcaccg
     2281 acgaaacgcc gatcgcaatc acgaacgcgc tttcggaaaa cattgaagca atgcgcctct
     2341 cgctcatccc cggcctcctc gagacagtcg tcttcaatcg ctcgtatgga acgcgcgacg
     2401 gcgcgctgtt cgaggtggga cgcacgtacc atcgtagggg tgtggggagt ggggtgtggg
     2461 gagtggaaga aaagacaaac acctccacgc ccacaagcga cgcggcctcc gttgcaagaa
     2521 acaaaacgac gaccaacgca tcaaacacgt tctcccacac tccacccccc actccccaca
     2581 ccccagtcaa agagcaccac cgcgtcgccg tcgtgatgta cggctcgatc ggaacgttct
     2641 ggggcgatgc gaagcggacg gtcgatttct tcgacatcaa agggatcgtc gagcagatcg
     2701 cgaagaagct tcatgtcgac gtgacgttcg cggcgtccga tcagccgtgg ctgcgcggcg
     2761 ggaagcgcgc catcgcctgg catggcgacc ggcaaatcgc tagtctcgga ttcctctccg
     2821 ccgagcttct gcagaaattc ggaatcaagg gcgatgtggc ggccgcggag atcgacatcg
     2881 aagcgctcat cgcgtcgtcg aataccgaat ggaagatggc gccggtagcg cgtttccccg
     2941 gcgtgcccat gatcctcgcg cttacgcacg gacgcgacct cgagtatcag cgcatcatcg
     3001 atacgatcca ttcattcaac gttccccacc ttcacgaagt cggtcttcgc gaccgcttcg
     3061 tccccgaagg cgacgacgtg atcaagacca cgctgggcat gtggtaccag gcgttcgacc
     3121 gctcgctgac gcaggaagag atcgccggct tccagcagca gctcgcgacg cgcctcgccg
     3181 caaccctgcc ggttaaacta ctttgagaga gaccaacgtg aaaaagaaga gcgacgacca
     3241 actgagcatt gccggaaccg aggaggctga gatcctcggc cgcctgaacg accgcgtaga
     3301 aaaagcgatt accacgattc aagcgctgcg gaaagaacgc gacacactcc gccgcgagct
     3361 cgacgaagcc aacgcccgca tccaggagca gggcgacagt gccgaacgcg cctcgacgct
     3421 cgaagaagac aacgagcgat tcaagcgtga gcgctccgag atccgcgacc gcatcgagtc
     3481 gatcctcggc aatctggaag ctctcgagga gtagtcaagg agtgcaggcg tctcgccagg
     3541 cgtctcgcca ggcgtctcgc ctgcggacca gcgggcgccc tcgcccggtg gtaattccgt
     3601 gaccttgtcg tcgacgccgc agtgcgtatc tccgacattc gcgtattgac ctgcttcgta
     3661 ccgaaagcgt tgcggccgcg ctcgaacgtt gccgagaatc gcgaggaccc tccccgcacg
     3721 gtgaggcgag gacgcctcac cgccgcaggc gagacgcctg gcgagacgcc tgcactcctt
     3781 acacctccgc gtgaaaccgt tatcccctca acacgccgcg cgagaatttg atgaaatagt
     3841 cgacgccgga gacgagcgcg aagagcatga ctacccaaag cgccagctcg ccgatgaagc
     3901 ggaactcgcc cagctcgtag ccgatgatca gcagcgcgat ggcgacgacc tgcgcgacca
     3961 tcttcgtctt gccgagcggc gacgcggcga tcgtcacacc gtgctgtgcg gcgatcgaac
     4021 gaaggcctga caccgcaaac tcgcggccga tgatgatgac gatcatccag gcgcgggcca
     4081 ggccgagctc gatcagcgaa atgaacgccg cagacatcag gagcttgtct gcgatcggat
     4141 cgaggagcgc gccgaggcgc gtcatcttgt tgtacttgcg cgcgaagtag ccgtcgaaat
     4201 agtcggtgat ggcggcgacg ccgaagatgg ccaggccgag gtagttcgag tacttggtca
     4261 ggaggacgac gaccaggaag ggaacgagaa aaatgcgcag gagcgtcagc gagttcggga
     4321 ggttccagat ctcggcggcg acgtcatccg gcgggggaac gacggtaggt tttgcgcgct
     4381 ccgccagcgg aggagcggct tcgatcggtt gcatggaagg ttctcggcag atatccggca
     4441 tcatcagaat aaccggtttg accagtagtg ggaagacggg atcgcggtcg gtagtggggc
     4501 aatttgaaaa ccggtcacca cagaggcaca gaggtcacag agatcccctc tgtgttctct
     4561 gtgtctctgt ggttgccgtt gcttcggtcg cccgccggaa tacaaaaccg ccgattgaca
     4621 ttccgacgcc tgcaaccgag aatctcggtc ccgctcagaa tgcgaaagtg gcggaattgg
     4681 tagacgcgca ggtctcagaa gcctgtggtc attagaccat gggggttcga ttcccccctt
     4741 tcgcaccaag agctccctcc tgaaaggtca ctgcccgtga ctcccaactc cgctacgagc
     4801 tcagggaagt ctcgctgcgc gatgccggag cggcgcttcg atttgtgccg tgaagagcag
     4861 ccggttcggc gttccaactc cggggtcagc aaccttgttc agcgacgcac tcgcaatcag
     4921 cgcagacgcc acggttgccg gcgatgccgt gggatgatcc tgcacataga gcgctg
//
LOCUS       DATLVW010000224         7301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_297445, whole genome shotgun sequence.
ACCESSION   DATLVW010000224 DATLVW010000000
VERSION     DATLVW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297445"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1395
                     /locus_tag="VNN25_07225"
     CDS             190..1395
                     /locus_tag="VNN25_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXH91355.1"
                     /translation="MPEFIVRVGTPDGVITERTVQAMSIRAAEEDLRQQGMHVFDARR
                     GRMSLRDLLPRGGKIISTERFLLFNQELLALVKAGLPILQSFDIMLERQKNLRFKEVL
                     TDVREKLKSGIALSDAFASYGDAFPPIYSTSLRAGERSGDLEGVLRRFLRYQKIIVNL
                     RKRVVGALIYPSVLVLLSIGMVLVMLTKVIPKFAEFYAGFGADLPWFTQFMINIANGL
                     NTNLPIFLVICAAGFFFTRRWIRGGGRVTWDRLKLKIPLAGGILHRFAIMQFTQSLGT
                     LLAGGTPMVPAIEIASQSVTNQLVASKIFGMVQNVREGEPLWRSLENTKAISDLAIEM
                     IKVGESTGALTEMLGNVSEFYDEEIESHLTRIVAAIEPLILVFMGGVIAILLYAFYLP
                     LFQLSNVAQ"
     gene            1399..3024
                     /locus_tag="VNN25_07230"
     CDS             1399..3024
                     /locus_tag="VNN25_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXH91356.1"
                     /translation="MATDPRTLLYKTADPAGETARAQRMAAQYGFDFVNLEHFVVDHE
                     LFRNIPLELMIRYQFLPEKRENGHIAVVVGDPTDVLKLDELELLLGAPLKVRVASPAA
                     IRDKLQKSESTQRVLDEATEGFRMTLVGEDEEGEETLTIDSITAQESSPIIKLVDSIV
                     FNAIQRRASDIHIETRDNEVVVKYRIDGVLYEALEPIDKRHHTTIISRIKVMSELDIA
                     EKRVPQDGRFKLRITGRTIDFRVSIVPTTHGEDSVIRILDKESMNSDFKSLRLDVLGF
                     DEEMLLKFRKFIKEPYGMVLVTGPTGSGKTTTLYAALSEIQTSEDKIITIEDPVEYQL
                     RGITQIPVNEKKGLTFARGLRAILRHDPDKVMVGEIRDEETAAIAVQSALTGHLVFTT
                     VHANNVVDVLGRFLNMKVDLYNFVSALNCVLAQRLVRKICDSCRHEVSYPDSFIIESG
                     LDPKRYQGKMFQEGVGCIDCNGTGFHGRAAISELLDLSDRIRTMILDRRPASEIKKAA
                     KEEGMMFLRESALKKVMTGQTTLREINKVTFVE"
     gene            3026..3916
                     /locus_tag="VNN25_07235"
     CDS             3026..3916
                     /locus_tag="VNN25_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91357.1"
                     /translation="MARSYPPDVVVLDRDSLIHVRVGRGRKDAKILQAKSYALRDDVF
                     APGVVTPALQNEAVFADVLRRLKLETGRWDKVSILVPDSWIRMNIIELQSVPERASEV
                     EEVIRWSLRRTMPIDPSLLRLSYEVLTRTPPRVLAFSAVEATLASIERLFAAAGVEVI
                     LLEPCGLNIWNAITSREALTTRDRIFFYIRANDFTTAVFRGSQPLFIRSRNLDGERSL
                     EQEIKLSATYLRDSLQTSAIENCYVAGNDVSGSVVDAIGAEFSAPVRKITLRDVAEQV
                     PGDSSQYEAELTAATGVFTG"
     gene            3913..4491
                     /locus_tag="VNN25_07240"
     CDS             3913..4491
                     /locus_tag="VNN25_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91358.1"
                     /translation="MKPIHLNLASRPFRDYRPVYAAVVVMALLTAFLALNNVDTLLRY
                     RTETKTTRADISQLEQQTADERQKADALAQRLRGVDLKLLSTQTEFANTQLAERAFSW
                     SELLDRLERVLPDDVRLQSVTPTFDKDGLVHLTMMCVAKSGTGLSSTINHFNGDSHFA
                     NAFPSSEVVEPSGERRISLSVDYRPTITRSIE"
     gene            4488..5087
                     /locus_tag="VNN25_07245"
     CDS             4488..5087
                     /locus_tag="VNN25_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91359.1"
                     /translation="MIWREKRILLIVLGVLLLANTLFFFTYRVQYVSRLQDLDATKEQ
                     AQGRLQQARLARVTAEQQLAAYKKVQTDLQVLYNERWATPMQRLTTLIDEVKKLSAAS
                     HLEPPSYAFSSSETKTTSVKGSIGTTTVGITYTVVGTYEQIRRLINLLELSDQFVIID
                     GINLVSSGVAADKTLTLNIRLKTLFRDTRGIAPTANRQM"
     gene            5096..5779
                     /locus_tag="VNN25_07250"
     CDS             5096..5779
                     /locus_tag="VNN25_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXH91360.1"
                     /translation="MNWKTWAVIGAILIAIFAIYGYASNSGGGDTTPPLTAATQPRKP
                     ASGNAIRTSTAASTPGVPTLHIELLDPQSGSYKSTRNLFTFKEPPPPPPPPPVKVAPP
                     PPDQDHDGIPDFRDNCPTVYNPDQADSDHNGKGDACDEEWKAYKLLHPDPPPKPVPPQ
                     FTLKYIGTFGPASSPIATFNGNGQIVNVRVGETFDGKFILLSIGIESVEIGYVGFPTD
                     VKTRVPLGQ"
     gene            6006..>7301
                     /locus_tag="VNN25_07255"
     CDS             6006..>7301
                     /locus_tag="VNN25_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXH91361.1"
                     /translation="MNQTRLGITALVLSLTMFSCTSYNAFQKAKHAETSKDWDAAVTE
                     YQKALDVDPSNALYRINLSRAKLEASRVHFEKGKDLRAAAASARGADVVRLMQLAATE
                     LEIVIRLDPTNQFAASEYSKTVATLQDIANAGRPASIDELKRRAKANITKSAPPQLSP
                     TSDQPISLSFPQERPIKEIYKALGNAFGINILFDQAVKDDRIAIELKDVTAQQALERV
                     MQAANHFYKALDEHTIIVVPDNPQARRDYEDLVIRTFYLSNGDAEQVTNVVRTMIEAR
                     NVFPLKALNAITIRDTADKVRIAEKIIEANDKARAEVVVDVELLQIDLDKQRDIGAVV
                     QGLGSAAAPGTFGVDLPKDKDGNTLPSNLDSLRALKSANFLFTIPSISYNFIKSVGNT
                     ETLANPELRISEGEKATLHIGQRVPVPVTTFTTGVQSGSL"
BASE COUNT         1569 a         2432 c         2003 g         1297 t
ORIGIN      
        1 gacatgtcgg gaacctttcg tgacctgatc cgtcgaagag tcaggacacg tcgggaacct
       61 ttcgtgacgt gatccgtcga agagtcagga catgtcagga acctttcgtg atgtgatccg
      121 tcgaatagtc aggacacgtc aggaaccttt cgtgatgtga tccgtcgaat agtcagcgga
      181 aacgaacaaa tgcccgagtt catcgtcaga gtcggaacac ccgacggagt catcaccgag
      241 cgcaccgtgc aggccatgag catccgcgcg gccgaagaag acctgcgcca gcagggaatg
      301 cacgtcttcg atgcccggcg gggccgcatg tcgctccgcg atctgcttcc tcgcggcgga
      361 aagatcatct ccaccgaacg attccttctc ttcaaccagg agcttcttgc gctggtcaag
      421 gccggcctgc ccatcctgca gtcgttcgac atcatgctgg agcggcagaa gaatctccgc
      481 ttcaaggaag tgctcaccga cgtccgggag aagctgaagt cgggaatcgc cctctccgac
      541 gccttcgcct cctacggcga tgccttcccg cccatctatt cgacttcgct gcgcgccggc
      601 gagcgatcgg gcgatctcga aggcgtgctg cggcgctttc ttcgctatca gaagatcatc
      661 gtcaacctgc gaaagcgggt cgtcggcgcc ctgatctatc cctccgtgct cgtgctcctc
      721 tccatcggca tggtgctggt catgctgacg aaagtcatcc ccaagttcgc cgagttctat
      781 gccggcttcg gggcggacct cccctggttc acgcagttca tgatcaacat cgcgaacggg
      841 ttgaacacca acctccccat cttcctcgtt atctgcgccg ctggattctt tttcacccgg
      901 cgttggatac gaggaggggg aagggtgacg tgggacaggc tcaagttgaa gattcccctg
      961 gccggcggaa tccttcacag attcgccatc atgcagttca cgcagtcgct gggtacactt
     1021 ctggccggcg gaacgccgat ggtgcccgcc atcgagatcg cctcgcaatc ggtgaccaac
     1081 cagctcgtag cctcgaagat cttcggaatg gtccagaacg tgcgcgaggg cgagccgctg
     1141 tggcgctcgc tcgagaacac caaggccatc agcgatctgg ccatcgaaat gatcaaggtg
     1201 ggggagtcga ccggcgccct cacggaaatg ctcggaaatg tcagcgagtt ctacgacgag
     1261 gaaatcgaat cccatctcac gcgaatcgtt gcggcaatcg agccgctcat cctcgttttc
     1321 atgggcggcg tcatcgccat tctgctatac gccttctatc tcccactctt ccagctcagc
     1381 aacgtagccc aataaaacat ggccaccgat cctcgcacac tgctctacaa gactgccgac
     1441 cccgccggcg agaccgcgcg cgcccagcgc atggccgccc agtacggctt cgacttcgtg
     1501 aacctcgagc actttgtggt cgatcacgag ctcttccgca acatcccgct cgaactgatg
     1561 atccgctacc agttcctgcc cgagaagcgc gagaacggcc acatcgccgt cgtcgtcggc
     1621 gatccgacgg acgtcctcaa gctcgacgag ctggagctgc tcctcggcgc accgctcaaa
     1681 gtccgcgtgg cgtcaccggc ggccatccgc gacaagctgc agaagtccga atcgacccag
     1741 cgcgtcctcg atgaagctac ggaaggcttc cgcatgacgc tcgtcggcga agacgaggaa
     1801 ggcgaagaaa cgctgacgat cgactcgatc acggcgcagg aatcgtcgcc catcatcaag
     1861 ctggtcgact ccatcgtctt caacgccatc cagcgccggg cctcggacat tcacatcgag
     1921 acccgcgaca acgaggtcgt cgtcaagtac cgcatcgacg gcgtccttta cgaagcgctc
     1981 gagccgatcg acaaacgcca ccacaccacc atcatctcgc gcatcaaggt catgtccgag
     2041 ctcgacatcg ccgagaagcg cgtcccgcag gacggacgct tcaagctccg catcaccggc
     2101 cgcaccatcg acttccgcgt ctccatcgtc cccaccacgc acggcgaaga ttcggtcatc
     2161 cgtatcctcg acaaagagtc gatgaactcc gacttcaaga gccttcgtct ggacgttctc
     2221 gggttcgatg aagagatgct gctcaagttc cgcaagttca tcaaagagcc gtacggcatg
     2281 gtgctcgtca ccggccccac cggctcgggc aagacgacca cgctctacgc cgctctctcc
     2341 gagattcaga cctcggaaga caagatcatc accatcgaag acccggtcga gtatcagctt
     2401 cgcggcatca cgcagatccc ggtcaacgag aagaagggac tgacgttcgc gcgcggtctc
     2461 cgggccatcc tccgtcacga ccccgacaag gtcatggtcg gcgagatccg cgacgaagag
     2521 acggccgcca tcgccgttca gtccgcattg accggccacc tcgtcttcac caccgttcac
     2581 gcgaacaacg tcgtcgacgt tctgggccgc ttcctcaaca tgaaggtcga cctttacaac
     2641 ttcgtttccg cgctcaactg cgtactcgcg cagcggctcg ttcgcaagat ctgcgactcc
     2701 tgccggcacg aggtgtccta tcccgactcg ttcatcatcg agtcaggtct cgaccccaag
     2761 aggtaccagg ggaaaatgtt ccaggaaggc gtcggctgca tcgattgcaa cggcaccgga
     2821 ttccacggtc gggcggcgat ctcggaactt ctcgacctct ccgatcgtat tcgaaccatg
     2881 attctcgacc gccgtcccgc ttcggagatc aaaaaagcag cgaaagaaga gggaatgatg
     2941 ttcctacgcg aatcggccct caaaaaagta atgacaggac agaccacgtt gcgtgagatc
     3001 aacaaggtca cgttcgtgga gtgatatggc gcgctcctac ccaccagacg tcgtcgttct
     3061 cgaccgggat tccctcatcc acgtccgtgt aggacggggc cggaaagacg cgaagattct
     3121 tcaggcgaag agctacgcgc ttcgcgatga cgtcttcgcg cccggcgtcg tcacgcccgc
     3181 tctgcaaaac gaagcagtgt tcgccgatgt attgcgccgc ctcaagctgg aaacgggacg
     3241 gtgggacaag gtctcgatcc tcgtccccga ctcctggatt cggatgaaca tcatcgagct
     3301 ccagtccgtg cccgagcgcg ccagcgaggt cgaagaggtc attcgctgga gcctgcgccg
     3361 gacgatgccg atcgatccgt cgctgttgcg cctctcgtac gaggtattga cccgcacgcc
     3421 tccgcgcgtg ctcgccttca gcgcggtgga agcaacgctc gcctcgatcg agcgcctctt
     3481 cgcagctgcc ggcgtcgagg tcatcctcct cgagccctgc ggcctgaaca tctggaatgc
     3541 catcaccagc cgggaagcat tgacgacgcg cgaccggatt ttcttctaca tccgggcgaa
     3601 tgacttcacc accgccgtct tccgcggctc gcagccgctg ttcatccgtt cgcgcaacct
     3661 ggacggggag cgctcgctcg aacaggagat caaactctcc gcaacgtatc tccgggattc
     3721 tctgcagacg agcgcgatcg aaaactgcta cgtcgccggc aacgacgtga gcggaagcgt
     3781 ggtggatgca atcggcgccg agttctccgc gcccgtcagg aagatcacgc tgcgcgacgt
     3841 ggcggaacag gtgcccgggg acagcagcca gtacgaagcc gagctgaccg cggccacagg
     3901 agtattcacc gggtgaaacc gattcatctc aatctcgcct cgcggccgtt ccgcgactac
     3961 cggcccgtct acgcggccgt ggtggtcatg gcactgctga ctgcatttct cgctctcaac
     4021 aacgtcgata cactgcttcg ctatcggacg gagacgaaga cgacgcgcgc cgacatcagt
     4081 cagctcgaac agcagactgc cgacgagcgg caaaaagcgg acgcgctcgc gcagcggctt
     4141 cgcggcgtcg atctcaagct tctgtcgacg caaacggagt ttgccaacac gcagctcgcc
     4201 gagcgcgcat tctcgtggag cgagctgctc gaccgcctcg agcgtgtcct ccccgacgat
     4261 gttcgccttc agtcggtaac cccgacgttc gacaaggacg ggctcgttca cctcaccatg
     4321 atgtgcgttg ccaaatccgg caccggcctt tcctcaacga tcaatcactt caacggtgac
     4381 tcacacttcg ccaacgcgtt cccctcgagc gaggtggtgg aaccgtccgg cgagcgccgc
     4441 atctcgctca gcgtcgatta ccggcccacg atcaccagga gcatcgaatg atctggcgtg
     4501 aaaaacggat tctcctgatc gtcctcggcg tgcttctgct cgcgaacacg ctctttttct
     4561 tcacgtaccg cgtgcagtac gtgagccgcc tccaggacct cgacgccaca aaggaacagg
     4621 cccagggccg tttgcagcag gctcggctcg cgcgcgtcac ggccgagcag cagctcgcgg
     4681 cgtacaagaa ggtgcagacc gacctgcagg ttctctacaa cgaacggtgg gcgacgccca
     4741 tgcagcggct gacgacgctg atcgacgagg tcaagaaact ctccgcggcc agccacctcg
     4801 aaccgcccag ctatgccttc tcgagctcgg aaacgaagac gaccagcgtc aaaggatcca
     4861 tcgggacgac cacggtcggc atcacctaca cggtcgtggg aacgtacgag cagatacggc
     4921 gcctgatcaa cctgctggag ctctccgatc agttcgtgat catcgatgga atcaacctgg
     4981 tcagcagcgg cgtggcagcc gataagactc tcaccctcaa cattcgactc aagaccctgt
     5041 tccgcgatac gcgcggcatc gcgccaaccg cgaacaggca gatgtgatgg actggatgaa
     5101 ctggaaaacc tgggcagtga tcggtgcgat tctcatcgcg atcttcgcga tctatggcta
     5161 cgcctcgaat tcgggcggcg gcgataccac accgcctctt accgccgcga cccaacccag
     5221 gaaaccggcg tccggaaatg caattcgcac gtccacggcc gcgagcacgc ccggagtgcc
     5281 gacgctgcat atcgaactgc tcgatcctca gagtggcagc tacaaaagca cgcgcaatct
     5341 cttcacgttc aaagaaccgc cgcctccgcc gcctccgcca ccggtgaaag tcgcaccgcc
     5401 gccgcccgat caggatcacg acggcatccc tgatttccgc gacaactgtc cgacggtcta
     5461 caaccccgac caggcagaca gcgatcacaa cggcaaaggc gacgcctgcg acgaagagtg
     5521 gaaagcttac aaactgcttc atccggatcc gccgcccaag ccggttccgc cccaattcac
     5581 attgaagtac atcggcacgt tcggccccgc cagcagtccg atcgcgacgt tcaatggcaa
     5641 cggccagatc gtcaacgttc gggtaggcga gaccttcgac ggaaagttca tcctgctgag
     5701 catcgggatc gagtccgtgg agatcggcta cgtcgggttt ccgacagacg tcaaaacacg
     5761 cgtgccgctc ggccagtgac cttttgaatc gcgaatcgtc taagtattca gataaggagg
     5821 gagtcgggct caggagccgg agaaaacgag acaaagagac cgttcagcga cgagagatca
     5881 gcgacaagag atcagaccga aagttccctg atctctaatc tctgatctcc agatctctga
     5941 agcactgatc tctgaaacaa gacgtctccg actcccggcc cccgacctcc cactcccgta
     6001 aaaacatgaa ccagaccaga ctcggaatca cagcattggt gttgtcgctc acgatgttct
     6061 cctgcacgag ctacaacgcg tttcagaagg ccaaacacgc cgaaacgtcg aaggattggg
     6121 acgcggccgt caccgagtac cagaaggcac tcgacgtcga tccatcgaat gcgctctacc
     6181 ggatcaatct gtcgcgcgcg aagctcgaag cgtcgcgcgt gcatttcgag aaggggaaag
     6241 acctgcgcgc cgccgccgcg agtgcgcgcg gagccgatgt tgtccggctc atgcagctcg
     6301 ccgcaacgga actggagatc gtcatccggc tcgatcccac caatcagttt gccgccagcg
     6361 aatacagcaa gaccgtggcg acccttcagg acatagccaa cgccggacgg cctgcctcga
     6421 tcgacgagct caagcgccgc gccaaggcca acatcacgaa gtcggcaccg cctcagctca
     6481 gccccacctc cgatcagccg atctccctct cctttcccca ggagcggccg atcaaagaga
     6541 tctacaaggc acttggaaac gccttcggaa tcaacatcct cttcgatcag gccgtgaagg
     6601 acgaccgcat tgccatcgaa ctgaaagacg tcaccgcgca gcaggccctc gagcgggtca
     6661 tgcaggccgc caatcatttc tacaaagcgc tcgatgaaca cacgatcatc gtcgttcccg
     6721 acaatcccca ggcccgccgc gactacgagg atctcgtcat ccgaacgttc tatctctcga
     6781 acggagatgc cgagcaggtc acgaacgtcg tccgtacgat gatcgaggcg cgcaacgtct
     6841 tcccgctcaa agcgctcaac gccatcacca ttcgcgacac cgccgacaag gtgcgcatcg
     6901 ccgagaagat catcgaagcg aacgacaaag cgcgggccga agtcgtcgtc gacgtcgagc
     6961 tcctgcagat cgacctcgac aagcagcgcg acatcggcgc ggtcgtgcaa ggcctcggtt
     7021 ccgccgccgc tcccggaacg ttcggagtgg acctcccgaa ggacaaggac ggcaacacgc
     7081 ttccctccaa cctcgattca ctccgcgccc tgaagagtgc caatttcctc ttcacgatcc
     7141 cgtcgatctc ctacaacttc atcaagagcg tcggcaacac cgagacgctt gcgaaccccg
     7201 agctccgtat ctccgaggga gagaaggcca cgctgcacat cggccagcgc gttcccgttc
     7261 cggtcacgac attcaccacc ggcgtgcaga gcggatcgct g
//
LOCUS       DATLVW010000225         5509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_90913, whole genome shotgun sequence.
ACCESSION   DATLVW010000225 DATLVW010000000
VERSION     DATLVW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90913"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VNN25_07260"
     CDS             <1..610
                     /locus_tag="VNN25_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HXH91362.1"
                     /translation="CDTGRVVTFTNWECGFGYRQSIFKSSAKDRYIVAGMTFRLTPGG
                     AAAIRYPELQTYLDEHEIDVSDLRGVRSAVIAIRKRKGMVLDATDPDTRSDGSFFMNP
                     IVTQSTLDEIVLRAGTKNVPHFPSGDQIKLSAAWLIEHAGFHKGFAHGNVGLSSKHTL
                     AVINRGGGTAREVLELVAMIQGGVREEFGVEIQPEPNFIGFK"
     gene            complement(702..1136)
                     /locus_tag="VNN25_07265"
     CDS             complement(702..1136)
                     /locus_tag="VNN25_07265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXH91363.1"
                     /translation="MRFSVSEYVRWEDIDAAGIINYQAYLRFFGLAEVELFRSCGLSY
                     RTLFDELGLWLPRVHVECDFFHPVTLDELLIVDAYFGKIGSSSIHLNFEVHRSEAPDI
                     IVATGKYVLVAVKRGEFKPAAVPDKVREKLAKYVEPVVSLPH"
     gene            complement(1298..1771)
                     /locus_tag="VNN25_07270"
     CDS             complement(1298..1771)
                     /locus_tag="VNN25_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91364.1"
                     /translation="MDRVFDERREKALQRRTVPRGCHGHFRDLRVKMDLLCRCHGSNA
                     SDGIVKRTPEIDSGHFQTVYFGVGAGQCERDNTFLRFASPTLDSSVYDFSRAGLLLGA
                     TAVQECVMSPRSMTSASEGTAPPMDSILFPRTTITAEDWTMQILGAINLILRWYE"
     gene            1828..2058
                     /locus_tag="VNN25_07275"
                     /pseudo
     CDS             1828..2058
                     /locus_tag="VNN25_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2078..2455
                     /locus_tag="VNN25_07280"
     CDS             2078..2455
                     /locus_tag="VNN25_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXH91365.1"
                     /translation="MPLVKIYVRRNIAEADLHAISGAVHEALVAEANVPPDDRFHILN
                     RLDVNEIVAHPTYGGVNRSDEIVVIEITLNAGRTVEVKKNLYAGIAKRLEALGVRPDD
                     VLVSLIEVTKENWSFGGGRATYA"
     gene            2622..5117
                     /gene="bamA"
                     /locus_tag="VNN25_07285"
     CDS             2622..5117
                     /gene="bamA"
                     /locus_tag="VNN25_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXH91366.1"
                     /translation="MNRIVAISLAAGILAGSALAQAVPEPQTPAKPKPGTIIDRPDSG
                     HISSEPVLSPAPAPVTPQPGAPTGQRIERFEASGNTTVATDTIRVYLGVNQGDPYDPV
                     AIQRNFLNLWQTGLFDDIRLETDAAPNGGVIVRAVVKERPRIGAVEYRGNKELNTAKI
                     NEQLDKDKIDLHVGNTVEQTLVRRAAESIKKAYSEGGFEGVTVDTTMEDMPDGEKKIV
                     FNVSEGIKATVARVFFTGNNHFSTRVLRRQMKEVKENNIITWIRKKNLYIPSKLDEDL
                     EHIKNYYQDFGYTSVAFGEPQVVTTGKLKKQRVSITIPIKEGTIHHLGEVSVTGATLL
                     KPDAFTGNFPLKKGDVIRRKVIQDRIDAIDEIYRARGYIYSYINPEYVEKDANVVDVH
                     IQVYEGDQFRLGRLEFQGNNTTKDKVLRREIFLDEGGIMDMETFKQSIYKLGQLGYFK
                     VNDNPDFKVNQEKKTVDVTVKGTEEGKNDVQFGGGYSEGTGFFVQTQFSTRNFLGEGE
                     NLGVSFQRGNRQNFYSLSYSDPWFMDTPNSLGISLFNRDTNYPLSIGYQEQSKGGSIA
                     YGYRLHRFDSLSLVYGLEHVKTHEETNVAPDLNGNVPISDILDATYTSSSIGPSYSYD
                     SRDNPFDTTRGARVAMGMTFSGGPLGGTIHAIRPTFATTKYFKISKRSSFSVNVDLGY
                     LRPLDYGKGCFLTYDNFEQNSKLCVPKGQRFFVGGEYSVRGFEYGTLGPNETVNGVTQ
                     VAGGYKQVFFNSEYVIKINDPLRLVFFGDGGWAYGYKDDLDPHKLRYSTGAELRIFLP
                     VFQFPIRFIYAINPASKPGDKFKTFNFTIGNTY"
     gene            5172..>5509
                     /locus_tag="VNN25_07290"
     CDS             5172..>5509
                     /locus_tag="VNN25_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXH91367.1"
                     /translation="MKKLLVSLAVAALAAPMFAQSAPARFAVIDVNKVLMSSAPGKAA
                     YERLKKMQDDRIAKATAMNDEIAKLDNDINTKKLTLSEDKLTDMAKQLSDKKIAAQRF
                     AQDADREVGEA"
BASE COUNT         1219 a         1716 c         1580 g          994 t
ORIGIN      
        1 gtgtgatacc ggccgcgtcg tgacgttcac gaactgggag tgcggcttcg gctaccggca
       61 gagcattttc aagagctcgg cgaaggatcg ctacatcgtc gccggtatga cgttccggct
      121 gactcccggt ggggcagcgg cgattcgcta tcccgaattg cagacatatc tcgacgagca
      181 cgagatcgac gtcagcgacc tgcgaggagt ccgcagcgcg gtcatcgcca tacgcaagcg
      241 caaaggaatg gtgctcgatg ccaccgatcc cgacacgcgc agcgatggct cgtttttcat
      301 gaatccgatc gtgacgcagt cgacgctcga tgagatcgtt cttcgcgccg gcacgaagaa
      361 tgtgccgcac tttccgtccg gcgatcaaat caagctctcc gccgcctggc tgattgaaca
      421 cgccggtttt cacaagggtt tcgcgcacgg aaacgtaggc ctgtcgtcga aacacacact
      481 cgccgtgatc aaccgcggcg gcggcaccgc gcgcgaagtg ctggagctcg tggcaatgat
      541 tcaaggaggt gtacgcgagg aatttggcgt cgagattcaa ccggagccga atttcatcgg
      601 gttcaagtaa gcgcattcga cgttgaacca cagagacaca gagatcactg agttgaggct
      661 gtcctctgtg acctcggtgt ctctgtggtg gaagggttgg atcaatgcgg caatgacacg
      721 acaggttcca cgtacttcgc caacttctcc ctcaccttat ccggcaccgc cgccggcttg
      781 aactcgccgc gcttcacggc cacgaggacg tacttgccgg tagcgacgat gatgtcgggg
      841 gcttcgctgc gatgcacttc gaagttgagg tggatcgagc tgctgccgat ctttccgaag
      901 taggcgtcga cgatcaggag ctcatcgagc gtgacggggt ggaagaagtc gcactcgacg
      961 tggacacgcg gcagccagag gccgagctcg tcgaagagcg ttcgatacga gaggccgcac
     1021 gaacggaaca gctcgacctc ggccaagccg aagaagcgca ggtaggcctg gtagttgatg
     1081 atgccggcag cgtcgatgtc ttcccagcgg acgtactcgg acacggaaaa gcgcatatcg
     1141 atagaattct agtcacgaga caacttcttg ccgcgctctt tcttttgttc gcgtcgtcgc
     1201 tgttcgcggc cgatgctccg caaactctcg tagccatccg cgcggcgcgg ctcatcgacg
     1261 gacgcgacgg gagggtcgta tcgcctggcg tcgtagctca ctcgtaccag cgaagaatga
     1321 ggttgatggc gccgaggatc tgcatcgtcc agtcctctgc ggtgatcgtc gttcgcggga
     1381 acaaaatcga gtccatcggc ggtgccgttc cctccgacgc gctggtcatc gacctcggtg
     1441 acatgacgca ctcctggacg gcagtggccc caaggagcag tccggcgcgg ctgaagtcgt
     1501 agaccgaaga atcgagcgtc ggactggcga atcggaggaa ggtgttgtcg cgctcacact
     1561 ggcccgctcc gacaccgaag tagacagtct ggaaatgacc gctatcgatc tctggggtcc
     1621 tcttcacgat gccgtcgctc gcgttgctcc catggcagcg gcaaagcaag tccatcttca
     1681 cgcggagatc ccggaagtgg ccgtggcagc cccggggaac ggttcggcgc tgcaacgcct
     1741 tttctcggcg ctcatcgaaa acgcggtcca ttacacgccc gcagacggcg aggtactcgt
     1801 cacacccact gttgcacagg tgaagctgcc acgatcgtcg tccgcgacaa tggcgtcgga
     1861 atcgctgcct ccgacctgcc acacatcttt gagcgatttt accgagccga caagtcgcga
     1921 acccgccagt ccggaggcac cggccttggc ctccccatcg cccagcggat cgctgcactc
     1981 cacggcggaa cgatcaccgt ggccagcggc agagaaggtt cacagttcag cgttgcactc
     2041 ccgacacgca tcgagtagca gttagactgt cccgatcatg ccactcgtca agatctacgt
     2101 acgcaggaac atcgccgaag ctgacttgca cgccatcagc ggcgcggtcc atgaagcgct
     2161 ggtcgcggag gcgaacgttc cgcccgatga tcgctttcac atcctcaatc ggctcgatgt
     2221 taacgaaatc gttgctcacc ccacgtacgg aggcgtgaac cgctccgacg agatcgtcgt
     2281 catcgagatc acgctgaatg ccggaaggac cgtggaggtc aagaaaaacc tctacgccgg
     2341 catcgctaaa cgtctcgaag cgctgggcgt acggcccgat gacgtgctgg tctcgctgat
     2401 cgaggtcacg aaggaaaact ggtcattcgg cggagggcgg gccacctacg cctgaaaagc
     2461 ggttccgagg ctcatctctt tgctagaatg cgccgccgct ctggtctgtt gtgggctccc
     2521 gcgtaacctg cgtcctggtt cgcacgtcta catgttgaca ggatgaaaga gcaggtacgc
     2581 gtccggccag tcggagccgg cgccgatcct caataataac tatgaatcga atcgtcgcca
     2641 tctccctagc ggcgggcatc cttgcgggga gcgctctcgc gcaagccgtg cccgaaccgc
     2701 agactcccgc caaaccgaag cccggcacga tcatcgatcg tcccgactcc ggacacatct
     2761 cctccgagcc ggtcctatcg cccgctcctg ctcccgtcac gccccaaccg ggagcaccga
     2821 ccggtcagcg gatcgagcgc ttcgaagcgt ccggcaacac cacggtggcc accgacacga
     2881 ttcgcgtcta cctgggcgtg aatcagggtg acccctacga tcccgtggcg atccagcgca
     2941 acttcctcaa cctctggcag accggccttt tcgacgacat ccgtctcgaa acagacgcgg
     3001 cgccgaacgg cggcgtcatc gttcgcgcgg tcgtcaaaga aaggccgcgc atcggagccg
     3061 tcgaataccg cggcaacaaa gagctgaaca ccgccaaaat caacgaacag ctcgacaagg
     3121 acaagatcga cctgcacgtc ggcaacactg tcgagcagac gctggtgcgc cgtgccgccg
     3181 agtcgatcaa gaaggcttac tccgaggggg ggttcgaggg cgtcacggtc gacacgacca
     3241 tggaagacat gcccgacggc gagaagaaga tcgtcttcaa cgtcagcgaa ggcatcaagg
     3301 ccaccgtggc ccgcgtcttc ttcaccggca acaatcactt ctccacccgc gtcctgcgcc
     3361 ggcagatgaa agaggtgaag gaaaacaaca tcatcacctg gatccggaag aagaacctct
     3421 acatcccttc caagctcgac gaagatctcg agcacatcaa gaactactac caggacttcg
     3481 gatacacgag cgtcgcattc ggcgagccgc aggtggtcac gaccggaaaa ctgaagaagc
     3541 agcgtgtcag catcacgatc ccgatcaagg aagggacaat ccatcacctc ggcgaggtaa
     3601 gcgtcaccgg cgccacgctc ctcaagcccg acgccttcac cggcaacttc cccctgaaga
     3661 agggcgatgt catccgccgc aaagtcattc aggaccgcat cgacgcgatc gatgagatct
     3721 accgcgcgcg cggctacatc tactcctaca tcaaccccga atacgtcgag aaggacgcca
     3781 acgtcgtcga cgtacacatc caggtctacg aaggcgacca gttccgcctc ggacggctgg
     3841 agttccaggg caacaacacc acgaaagaca aggttctccg ccgcgagatc ttcctcgatg
     3901 agggagggat catggacatg gagacattca agcagtcgat ctacaagctg gggcagctcg
     3961 gctacttcaa ggtaaacgac aatccggact tcaaggtcaa ccaggagaag aaaacggtcg
     4021 atgtgaccgt gaagggaacc gaagaaggga agaacgacgt ccagttcgga ggcgggtact
     4081 ccgaggggac ggggttcttc gtgcagacgc agttttcgac gcgcaacttc ctcggcgaag
     4141 gggaaaacct cggcgtcagt tttcagcgcg gcaaccgcca gaacttctac tcgctctcct
     4201 actccgatcc ctggttcatg gacacgccga acagcctcgg catctccctc ttcaaccgcg
     4261 acaccaatta cccgctctcg atcgggtatc aggagcaaag caagggcggc tcgatcgcat
     4321 acggttaccg gctgcatcgc ttcgacagcc tctcgctcgt ctacggactc gaacatgtga
     4381 agactcacga agagacgaac gtggcgccgg acctcaacgg caacgtgccg atttccgata
     4441 tcctcgacgc aacctacacc tcctcgtcga ttggcccgtc ctattcgtat gactcgcgcg
     4501 acaacccgtt tgatacgacg cgcggcgcgc gtgtggcgat gggaatgacg ttctccggcg
     4561 gtcccctcgg cggcaccatc cacgccatcc gtccgacgtt cgcgacgacg aagtacttca
     4621 agatttcgaa gagatcgtcg ttcagcgtca acgtcgacct cggctatctc aggccgctcg
     4681 actacgggaa gggctgcttt ctgacgtacg acaatttcga acagaacagc aagctttgcg
     4741 tcccgaaagg tcagcgcttc ttcgtcggcg gcgagtattc ggtgcgcggc ttcgaatacg
     4801 gcacgcttgg cccgaacgaa actgtgaacg gtgtgacaca ggtcgccggc ggctacaagc
     4861 aggtgttttt caacagcgag tacgtcatca agatcaacga tccgttacgg ttggtgtttt
     4921 tcggtgacgg cggttgggcc tacggttata aggacgacct cgatccgcac aaattgcgtt
     4981 actcgacagg cgccgagctt cgcatcttcc tccctgtgtt tcagttcccc atccgcttca
     5041 tctacgccat caacccggcc tcaaaaccgg gtgacaagtt caagaccttc aacttcacca
     5101 tcgggaatac ctactaaacg cggggcgatt tagcgatcgt ccacgcccca aataaattaa
     5161 ggagattttc catgaagaag cttctcgttt cgctcgcggt ggcagcgctc gcggcgccca
     5221 tgtttgcgca gagtgcgccg gctcgctttg ccgtgatcga cgtcaacaaa gtgctcatga
     5281 gctcagcacc aggcaaggca gcctacgaac gcctgaagaa gatgcaggac gaccgcatcg
     5341 ccaaggctac ggccatgaac gatgagatcg caaagctcga caacgacatc aacacgaaga
     5401 agctcacgct cagcgaagac aagctgaccg acatggccaa gcagctctcc gacaagaaga
     5461 tcgccgccca gcgcttcgcg caggacgcgg accgcgaagt cggcgaagc
//
LOCUS       DATLVW010000226         6459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_90975, whole genome shotgun sequence.
ACCESSION   DATLVW010000226 DATLVW010000000
VERSION     DATLVW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90975"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..725
                     /locus_tag="VNN25_07295"
     CDS             177..725
                     /locus_tag="VNN25_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXH91368.1"
                     /translation="MSTTAMTGPITGAASPRFNVKVAGILYFANMLAFVPAAYVHSRF
                     VIAAGTAAATSNALVIDPLYRLGLTFDLIAVASGLAVTALFYDLFKPVSGRLSLFAAY
                     MNLMGCAITAASCILHLVPLVILRGADYFPVFTAKLEALALLFLKLRVQTAAMGIVFA
                     GVYCLLIGFLIFRSTFLPAEER"
     gene            722..1408
                     /locus_tag="VNN25_07300"
     CDS             722..1408
                     /locus_tag="VNN25_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXH91369.1"
                     /translation="MTSRTNARLAGFTFLFYIAIGIAEMVLSSRISSGPNTAARLANI
                     AQHLPQMRLVTVLVMLMIFCALVLAVTLYALTRDADADLALLAMTCRVAEGVTAIVPA
                     IAKLGLLSLASAAANTTRQEAASVNELASLLFRVGGWSTTVGATAFAVGSTIFAWLFL
                     RARSIPLWLAWVGLAGSLLMVVILPLQGIGLITNAVMSIGWAPLFVFEVTLAFWLLIK
                     GVTPRPVQQT"
     gene            1414..2145
                     /locus_tag="VNN25_07305"
     CDS             1414..2145
                     /locus_tag="VNN25_07305"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXH91370.1"
                     /translation="MTSITRPDNATISRPIEPEQHTAAKAVGFIYLIAMATSIFAEMY
                     ARAPLIVSGDAVRTAMNIVASERLFRIGSVIHLITFASDAMLAVALYVVLRPVNRNLA
                     LLAAAWRIVDCSVLSVNLLNDFVVLRLLSGADYLKVFDLKQLQALARLFYSVEGAGFQ
                     IGFVFLGLGSTLFSYLWLRSRYIPKVLAGWGIFASLVMAIVTLAVMLFPRLGEALGLT
                     YMAPMFFYEVGLGLWLLIKGIRAPI"
     gene            2172..2870
                     /locus_tag="VNN25_07310"
     CDS             2172..2870
                     /locus_tag="VNN25_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91371.1"
                     /translation="MTYSPILILHIAGGLTAIVAGFIALFVRKGGHVHRKSGDVFVIA
                     MLIMAAAGAYAAVIKSQPVNVVAGTFTFYLVASAWLTVRRKANETGRMEVGLLLLALS
                     ACVGAAILAWQASHMAKRGGPAPYVVFGLITFLSASGDIRLLIRGGVAGAQRIVRHVW
                     RMGLALFVATGSFFLGMAGDPVARRVGLRATLFTAEVRRTRLPLVPVLIVAVLTIFWL
                     FKVKLSKRYKTAAA"
     gene            complement(2950..4710)
                     /locus_tag="VNN25_07315"
     CDS             complement(2950..4710)
                     /locus_tag="VNN25_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXH91372.1"
                     /translation="MRIRALAAFAFTLILSTVSVFAQGKPAGSVETRLAAQNALFEEA
                     WQTNLKLAPTFATAVGDYRYNDKLGDNSLAAVAQRHEIDAGFLAKIKAISPEGFSEED
                     RTSHDLFLRGLQERMDDYDLKDYEMPVTAQGGIHTGLADLPNSVPLDSVKHYEDYIAR
                     LHQIPKAFLQTEDVLRAGVKDHLVIVKFVAEKIPGQCAGVIAADPFLIPIKKMPATFS
                     DDDKKRLTDAITTTVNDEVLPAYKQFAAFITTDYIPAGRATLSIESLPDGKRRYQAAI
                     RRLTTTNLTPAEIHKIGLSEFDRIVGEMTALAKANGYADLASFRDHVENDPKYKPSSE
                     QAIVDDFDRYIKQMRPKLPQLFNVIPSAPVTVEPIPDFQKAAATHYQTGTPDGKRPGR
                     VSVAVSDYKSRSFLNDEAVAYHEGIPGHHMQLSIQQTLKGLPEFRKHGGNSAYTEGWA
                     LYAEQLGKEVGFYQDPGSDYGRLRSELFRAVRLVVDTGIHDMGWSRDQVVEYMRNSHA
                     VDEPTIQSETDRYISWPAQACSYKLGQLKIRELRMRAEKQLGSAFDIRKFHDEVLAGG
                     SLPLDLLDARITRWISAQKK"
     gene            complement(4820..6091)
                     /locus_tag="VNN25_07320"
     CDS             complement(4820..6091)
                     /locus_tag="VNN25_07320"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXH91373.1"
                     /translation="MHRLASAALLVLLATSAYAQTDAVAARVDCILREVPLIDGHNDL
                     AEQYEERVKDHLGQIDIRQDQRKLTPPLHTDIARLRQGRIGGQFWSVYVPTTLKGADA
                     VRAVLEQIDVVHRLAALYPDTFELASTADDIVRIHRSGKIASLIGVEGGHSIGNSLAV
                     LRQLYALGARYMTITHSDNDDFADSATADPVHGGLTPFGRLVIAEMNRLGMLVDLSHV
                     APKTMNDVLDITAAPVIFSHSSARALTDHARNVPDDVLRRVKENDGVVMATWVPTFVS
                     ERARARDAAKDAEEAGLKALYRGQPEKVKAMLADWGIANPAPRATVKDVADHIDHIVQ
                     VAGAEHVGLGADLDGITSTPEGLESVAAYPAVFAELIRRGYSDEQLKGIAGLNVLRVL
                     RKAEAVAARLQKEKPPSDVRIEEVDKPVAAH"
     gene            complement(6198..>6459)
                     /locus_tag="VNN25_07325"
     CDS             complement(6198..>6459)
                     /locus_tag="VNN25_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011824553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HXH91374.1"
                     /translation="GQAEVLIDGGIRRGSDVVKALCIGARAVLVGRAYAYGLGAAGGA
                     GVARSIDILRSGIVRTMKLLGCASVRDLDRSFVQMPSAWLEF"
BASE COUNT         1114 a         2024 c         1983 g         1338 t
ORIGIN      
        1 gcacggtagc ggagagatcc tcgatcagtt atcgcaactc agtcccgcac gattcctggg
       61 cgcccctggc ctctgggtcg ggctgctatt cgccgccgcc tgcctcgcag gagccgtacg
      121 tctgcgccac gaccgggaac cgatctgatt ttgatgaacg actaatggag caaaccgtga
      181 gcacaacagc aatgactgga ccgattaccg gagcggcttc gccgcggttc aacgttaaag
      241 tcgcgggaat cctctacttc gcgaacatgc tggcatttgt gcccgccgcc tatgttcaca
      301 gccggtttgt catcgctgcg ggcaccgccg ccgccacgag caacgccctc gtcatcgatc
      361 ctttgtaccg cttggggctc acgttcgacc tcatcgcggt cgcctccggt ctcgccgtga
      421 cggctctctt ctacgatttg ttcaaaccgg taagcgggcg tctctcgctg ttcgccgcct
      481 acatgaacct catgggatgt gccattacgg ctgcgagttg catccttcac ctggtcccgt
      541 tggtgatctt gcgaggcgct gattatttcc ccgtattcac ggcaaagctc gaggcgctgg
      601 cgctcctgtt tctcaaattg cgcgtgcaga ccgccgccat gggcattgtc ttcgccggcg
      661 tctattgcct cctcatcggc ttcctgatct tccgatcgac gttccttcct gcggaggaaa
      721 gatgacatcc cggacaaacg cgcgcctcgc cggtttcacg ttcctcttct acatcgccat
      781 cggcattgcg gagatggtct tgtcgtcgcg gatcagcagc gggccaaaca ctgctgcgag
      841 actcgccaac atcgcccagc acctgccgca aatgcgcttg gttaccgtgc tcgtgatgct
      901 gatgatcttc tgcgcgctgg ttctggcggt gacgctgtat gcactgacgc gcgacgccga
      961 tgctgacctt gcgctcttgg cgatgacttg ccgcgtggcc gagggggtga ccgcgattgt
     1021 gccggcaatt gcgaaattgg ggctgctgtc gctcgccagc gccgcagcca acacaacaag
     1081 gcaggaagcc gcctcggtaa acgagctcgc gtcattgttg ttccgcgtcg gaggctggag
     1141 cacgaccgtc ggcgcaaccg ccttcgccgt cggcagcacg atcttcgcct ggcttttcct
     1201 gcgagcccgc agcatcccgc tgtggctggc gtgggtcggc ctggccggct cgctcctgat
     1261 ggtagtcatt ctccctctgc aagggattgg cctcatcacg aacgccgtca tgtcgatcgg
     1321 ctgggcgcct ctattcgtct tcgaggtaac tctcgccttc tggctgttga ttaagggcgt
     1381 gaccccaagg ccggtgcaac aaacatgagc gcaatgactt cgattacccg acccgacaac
     1441 gcgacgatca gccgtcccat tgaacccgaa caacacaccg cggctaaagc ggtcggtttc
     1501 atttatctca tcgcgatggc cacctcgatc tttgcggaga tgtatgcgcg cgctcccctg
     1561 atcgtctccg gcgacgccgt gcgcacggcc atgaatatcg tcgcctcgga acgcctgttc
     1621 cgcatcggca gcgtcatcca tctgatcacc ttcgccagcg acgccatgct ggccgtggcc
     1681 ctttatgtcg tactgaggcc agtcaacagg aatctggcac tgctcgcggc tgcatggcgc
     1741 atcgtcgact gctctgttct ctcggtgaat ctgctgaacg attttgtcgt actgcggctc
     1801 ctgagcggcg ccgactatct gaaggtgttc gacctgaaac agttgcaggc gctggcgcga
     1861 ttgttctaca gcgtagaggg cgccggcttt cagatcggct ttgtatttct tggactggga
     1921 tctacgcttt tcagctattt gtggctgcga tcgcgctaca tcccgaaggt gctggccggg
     1981 tgggggatct tcgcgtcgct cgtgatggca atcgtcacgc tcgccgtcat gctgtttccg
     2041 cgtctgggcg aagccttggg gctgacctac atggcgccaa tgttcttcta cgaagtcggc
     2101 ctcggcctct ggctactcat caaaggcata cgagcaccga tttaacgggc cccgcgaaaa
     2161 ggatccctcc aatgacctac tcccccatcc tcatccttca cattgccggc ggtctcaccg
     2221 ctattgtggc cggcttcatc gcattgttcg tacggaaggg cggccacgtg catcgcaagt
     2281 ccggcgacgt cttcgtcatc gcgatgctga tcatggccgc ggccggcgcc tatgccgcgg
     2341 tcatcaagtc ccaaccggtg aatgtcgtag ccggaacatt cacgttctac ctggtcgcat
     2401 cggcatggct taccgtcagg cgcaaggcga acgaaaccgg ccgcatggag gttggcctgt
     2461 tgctgctggc attgtcggcc tgcgtcggcg cggcgatcct cgcctggcag gcctcgcaca
     2521 tggcaaagcg cggaggcccg gcgccctacg tggtctttgg tttgatcacc ttcttatccg
     2581 cttccggcga catccgcctg ctcattcggg gaggagtcgc cggtgcgcaa cgcatcgtgc
     2641 ggcatgtgtg gcgcatgggc ctcgccctct tcgtcgccac cggctccttc ttcctcggca
     2701 tggccggcga tccggtggcg cggcgagtcg gattgcgcgc gacactcttc accgcagaag
     2761 tgcgtaggac gcggctgcca ctggtgccgg tgctcatcgt cgcagtgttg acgatcttct
     2821 ggttgttcaa ggtcaagctc tcgaagagat acaagacggc tgcggcgtga gggtaaagca
     2881 atcaacacag agacactgag agcacagagt tgatctctgt gtcctctgtg tctccgtggt
     2941 gaaaaaagac tatttcttct gtgcgctgat ccagcgagtg atccgcgcat cgagcagatc
     3001 gagcgggaga ctgccgccgg caagcacctc gtcgtgaaac ttgcggatgt cgaacgcgct
     3061 gcctagttgc ttctcggcgc gcatgcggag ctcgcgaatc ttgagctggc caagcttgta
     3121 gctgcacgcc tgcgccggcc aggagatgta gcgatcggtt tccgactgaa tcgtcggctc
     3181 gtcgaccgcg tgcgagttac gcatgtactc gaccacctga tcgcggctcc atcccatgtc
     3241 atggatgccg gtgtcgacga ccagacgcac cgcgcggaaa agctcggagc ggaggcggcc
     3301 gtagtcggag ccgggatcct gatagaagcc gacctctttc ccgagttgct ccgcgtacaa
     3361 cgcccacccc tcggtgtacg cagagttgcc gccatgcttg cggaactcgg gcagcccctt
     3421 gagagtctgc tgaatcgaga gctgcatgtg gtgaccggga atgccctcgt gatacgcgac
     3481 cgcttcgtcg ttcaaaaacg agcggctctt gtagtcggaa accgccaccg aaacgcggcc
     3541 cgggcgtttc ccgtcgggcg tgccggtctg gtagtgcgta gccgcggcct tctgaaagtc
     3601 agggatcggc tcgaccgtca ccggcgccga cggaatcacg ttgaagagct gcggcagctt
     3661 gggccgcatc tgcttgatgt agcggtcgaa gtcgtcgacg atcgcctgct ccgacgacgg
     3721 cttgtatttc ggatcgttct ccacgtgatc gcggaagctg gccagatcgg cgtaaccatt
     3781 cgccttggcc agcgccgtca tctcgccgac gatgcgatcg aactcgctca gcccgatctt
     3841 gtgaatctca gcgggcgtga ggttggtcgt cgtcagtcgc cggatagcgg cctggtaacg
     3901 ccgcttgccg tcaggcaaac tctcgatcga aagggtggca cggccggcag ggatgtaatc
     3961 cgtcgtgatg aacgccgcga actgcttgta cgccggaagc acctcgtcgt tcacagtcgt
     4021 cgtgatcgca tccgtcaggc gcttcttgtc gtcgtccgaa aaggtcgccg gcattttctt
     4081 gatggggatg agaaacggat ccgcggcgat gacgccggcg cactgccccg gaatcttctc
     4141 cgccacgaac ttgacgatca ccagatgatc cttgacgcct gcgcgcagga cgtcttcggt
     4201 ctgcaggaac gccttcggga tctggtgaag gcgcgcgatg taatcctcgt aatgcttcac
     4261 ggagtcgagc ggcaccgagt tcggcaggtc ggccagcccc gtgtgaatgc cgccctgtgc
     4321 ggtgaccggc atctcgtagt ctttcaggtc gtagtcatcc atccgctcct gcagaccgcg
     4381 gaggaagaga tcgtgggagg ttcgatcctc ttcgctgaaa ccttccggcg agatcgcctt
     4441 gatcttcgcc agaaaaccag cgtcgatctc gtgccgctgc gcgaccgcgg cgagcgagtt
     4501 gtcgcccagc ttgtcgttgt agcgatagtc gcccacggca gtagcgaagg tcggcgcgag
     4561 cttgaggttg gtctgccacg cctcttcgaa aagcgcgttc tgcgcggcga gacgggtctc
     4621 gacggagccg gccggtttgc cttgggcgaa aacggaaacg gtggaaagga ttagggtgaa
     4681 agcgaaggcg gcaagagcac gtatgcgcat tcggagagcg tagcagactg acggacgacg
     4741 caaccacaga gctgccccgg acacagaaaa ttctcagtga cctctgcgtc tccgtggtga
     4801 acgcctcccc gtgcaccatt cagtgcgcgg cgacgggctt atccacctct tcgatgcgga
     4861 cgtccgaggg cggcttttct ttctgcagac gcgcggcgac cgcctcggct tttctcaaaa
     4921 cgcggagcac gttcaatccg gcgatgcctt tgagttgctc gtcgctgtaa ccgcgccgga
     4981 tcagctccgc gaagacggct ggatacgcgg ccacgctctc caggccttcc ggcgtagacg
     5041 tgatgccatc gagatccgct ccgagtccga cgtgttccgc gcctgcaacc tgcacgatgt
     5101 ggtcgatgtg atcggcaaca tccttcaccg tcgcgcgcgg cgcaggattg gcgattcccc
     5161 aatccgcgag catcgccttc accttctccg gctgaccgcg gtagagtgct ttgaggcccg
     5221 cttcctccgc atccttcgcc gcgtcgcgag cgcgcgccct ctccgaaacg aacgtcggaa
     5281 cccatgtggc catcaccacg ccgtcgtttt ccttgacgcg gcgcaagacg tcgtcgggaa
     5341 cgttgcgcgc gtgatccgtg agcgcccgcg cggacgaatg cgagaagatc accggcgcgg
     5401 cggtgatgtc gagtacatcg ttcatcgtct tcggtgcgac gtgcgagaga tcgacgagca
     5461 taccgaggcg gttcatctcg gcgatgacga gcctgccgaa cggcgtgagc ccgccgtgaa
     5521 ccggatcggc ggtggcggaa tcggcgaagt cgtcgttgtc gctgtgcgtg atggtcatgt
     5581 agcgcgcgcc gagggcgtag agctggcgga gcacggccag cgagttgcca atcgagtgac
     5641 cgccttcgac tccgatgagg gatgcaatct ttccgctgcg atggatgcgt acgatgtcgt
     5701 cagcggtgct tgccagctcg aacgtatcgg ggtagagcgc ggccaggcga tgcacgacgt
     5761 cgatctgctc gagcaccgca cgcacggcat ccgccccctt gagcgtcgtc ggaacgtaga
     5821 cggaccaaaa ctgtccgccg attctcccct gccgcagccg cgcgatgtcc gtatgcaacg
     5881 gtggcgtgag cttgcgctga tcctgccgga tgtcgatctg gccgaggtga tccttcaccc
     5941 gttcctcgta ttgctccgca agatcgttgt gcccgtcgat cagcggcacc tcgcggagga
     6001 tgcaatcgac gcgcgcggca accgcgtcgg tctgggcgta ggcggaggtg gcgagaagga
     6061 cgaggagcgc ggcagaagca agacgatgca tggcgcggat tgtagcggtg gactcgtttc
     6121 tcacgcgaag gcgcgaagga gagcatctca acctcaaaat ccttcgcgtc ttcgcgtctt
     6181 cgcgtgagaa ggttttctca gaattcgagc cacgcggaag gcatctgcac gaacgaccga
     6241 tcgagatcgc ggaccgacgc gcagccgagg agtttcatcg tgcggacgat gccggagcgg
     6301 agaatgtcga tcgatctcgc gacaccggct ccgcctgccg cgccgagtcc gtaggcgtac
     6361 gcgcggccga cgagaactgc acgtgcgccg atgcagagcg ccttcaccac atcgctcccc
     6421 cttcgaatgc cgccatcgat cagaacctcg gcctgcccg
//
LOCUS       DATLVW010000227         6927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_297635, whole genome shotgun sequence.
ACCESSION   DATLVW010000227 DATLVW010000000
VERSION     DATLVW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297635"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VNN25_07330"
     CDS             <1..1073
                     /locus_tag="VNN25_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91375.1"
                     /translation="MNIKRILTTTIAALLPLAVSATTLIVPASGTGPGANGSHWQTEL
                     TLHNTSASSATATLTFHDGLGAAGTSTIDIAPRATVAIADVVASRFGRESATGAIEIT
                     FDNALAQKLTIASRTFNTTSGGQLGQDIPALDAATAPSAGNTIVLGAPSSSADARFNF
                     GVYAVTAATVQWELLRADGSIAASKEVIYAAGSQKQYNDGVTTFFNVSAQNNDTVHAV
                     VTQGRALAYGSAINNASGDPTYVPGLLVHPDLRINFVGVSFGNAGAIDVEDANHDGVL
                     DHAIDIQTGSWPIGFRVVVNSSTGATFELVDPPSDVKLTDTHGGILWTPSASLSGTTT
                     TLTLRVTADGSTEIITIPVNLR"
     gene            complement(1209..2414)
                     /gene="tyrS"
                     /locus_tag="VNN25_07335"
     CDS             complement(1209..2414)
                     /gene="tyrS"
                     /locus_tag="VNN25_07335"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXH91376.1"
                     /translation="MTLDEQLDFLGKGTVDFIERKDLKTKLGRGKPLTIKVGFDPTAP
                     DIHLGHTVVIRKMKHFQQLGHRVIFLIGDFTGLIGDPTGKKATRPALSKEEVLENAET
                     YKQQVFKILDPKTTEIRFNSEWLGALGAEGMVRLAAKYTLARILERDDFRKRFDAHQP
                     IAIHELLYPLAQGYDSVALKCDVEMGGTDQLFNLLVGRDLMREYSLEPQVVITMPLLE
                     GLDGIEKMSKSLGNYIGINEDPGSIFGKVMSISDDLMWKYYTLCTDLTQAAIAEKRAA
                     VESGGLHPMDAKRELAKSIIRDFHDAAAADAAEAEFRRVFSERQAPAEIEETTLPAAS
                     EPQLLSKIIVQAGLSESNKDAQRVIAQGGVLVDDAKVNDPRATLDASSGKSYVLKVGK
                     RRFAKVSFG"
     gene            2795..3646
                     /locus_tag="VNN25_07340"
     CDS             2795..3646
                     /locus_tag="VNN25_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXH91377.1"
                     /translation="MRPLLAEETARRRRIELRFVDALTGAGFSEVVLPIIDYVEPYAA
                     LVEPATAKQSYRFVDREGDLIAIRSDFTPMLARALAPSIAPEELPLRLFYRGDVIRVE
                     ASRLGANRELFQIGAEIVGDDSIDADVAMLRLAADLVRAPGDEPTIVYNNVSIAAALI
                     AQAGAVREALAAKRMTAGVPAALQTIVAKLISGEATLNEVAPFAPEAAARLSAIEEAV
                     AGDAVFALHLDDIEDSGYYTGLRFRVYAGSARTKVAQGGRYDNLYERFGQRAAAIGFT
                     FTIDDLD"
     gene            3652..4263
                     /gene="hisG"
                     /locus_tag="VNN25_07345"
     CDS             3652..4263
                     /gene="hisG"
                     /locus_tag="VNN25_07345"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXH91378.1"
                     /translation="MRIAIPKGRLQERALSVFADAGYDIPSETDLKTRKLVFDRGGIE
                     WIFVKDGDVPVYVEHGAADAGIAGLDQILEHECTAYQPVEFPFGHCRMMLIAAPGAPP
                     LTKVATKYPRITRQFLDQRGVHAEIVPLTGSVELAAVLNLTSHVVDLVETGETVRIHK
                     LEMQEVVAEISPRLIVGKNFYRTEPKMVRELIAKIEEAARVSS"
     gene            4275..5483
                     /gene="hisD"
                     /locus_tag="VNN25_07350"
     CDS             4275..5483
                     /gene="hisD"
                     /locus_tag="VNN25_07350"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXH91379.1"
                     /translation="MRTFTTNTPESDEALRAILERRSATNENALRVADEMIAGVRARG
                     DAYVAEVIAKFDGVTLAPDAIRIAPRDVTISAEMKNAIDLAIDRVEAFHRPQLPQSYR
                     LGNLEHRVRPIRRAGVYVPGGRAVYLSTLIMTAVPARIAGVEEIVCITTSSAADRDEL
                     HYVCNRLGVRDIYRCGGAAGVAAAAIGTQSLARVDKIVGPGNAYVTAAKMRLIGEVGI
                     DMTAGPTELIVIADDIAHPQFVIADLLAQAEHGADSEVVLLTTSAWLAEQMPERALSF
                     VVNSMDEAIAIADRIAPEHLSIHARDAASIGARVQNCGAIFCGPSAPPAAGDYVVGTN
                     HVLPTAGSARFFSPLGVYDFVKRANVISITAEELAEVSEAGERIATFEGLPAHAASMA
                     IRRTACLATS"
     gene            5465..6511
                     /locus_tag="VNN25_07355"
     CDS             5465..6511
                     /locus_tag="VNN25_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH91380.1"
                     /translation="MSRDVVAAMTAYTLELREAAIKLNQNESPTDFPQELKEKVLAEM
                     AARHWNIYPDFESMMLRGALAKTHGYDTANVLVGNGSNELLAATIPTFIGPGTPVVFP
                     KPTFALYEKLVAVAGGIALPIAVDPSTGLLPLDEMLHAIERCDNAVVIVCSPNNPTGG
                     VLPEGGVDALLATGATVLFDRAYGDFAEDTMPPLHDRLVTFSTFSKAWGLAGLRVGWL
                     ASTASTCREIRKVKLPYSLNIISEAVAATALEHGEIRTHNVAATIAERKRMFEAMQEI
                     EGVTPFPSRSNFIAFTTQRNAKDVFNALHADGILIRDVSAAVPNALRVSVGTPSENDA
                     FLAALNQTMKDERP"
     gene            6508..>6927
                     /locus_tag="VNN25_07360"
     CDS             6508..>6927
                     /locus_tag="VNN25_07360"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HXH91381.1"
                     /translation="MRTATITRTTKETNVSVTLDLDGDGTHSKIETPVGFLSHMLDVI
                     ARHGRVDLDIVSTGDTHIDYHHTVEDTALVLGEAFAQALGDKRGIRRFGHAYVPLDEA
                     LARVVVDFAGRPFLVFDVPFDREMLLIHKDFPFALVEE"
BASE COUNT         1296 a         2390 c         1994 g         1247 t
ORIGIN      
        1 caatgaacat caaacgcatc ctcaccacca ccatcgcagc acttcttccg cttgccgtca
       61 gcgcgactac tctgatcgtt cctgcctcag gcacgggccc gggagccaat ggcagccact
      121 ggcagacgga gctgacgctc cacaacacca gcgccagctc ggccacggca accctcacgt
      181 tccacgacgg cctcggcgca gcaggcacct cgaccatcga catcgcgcca cgtgccaccg
      241 tcgcaatcgc cgacgtcgtg gctagccgct tcggacgtga aagcgcaacg ggcgcaatcg
      301 agatcacgtt cgacaacgcg ctagcgcaaa agctcaccat cgcgtcgcgc accttcaaca
      361 ccacctcggg cggccagctc ggccaggaca ttcccgcact cgacgcagcc accgcaccct
      421 ccgccggcaa taccattgtc ctcggcgcac cttcctcctc agccgatgcc cgcttcaact
      481 ttggcgtcta cgccgtaacc gcagcgaccg tgcaatggga gcttctgcgc gccgatggca
      541 gcatcgccgc atcgaaggaa gtcatctatg cagccggttc gcagaagcaa tacaacgatg
      601 gggtcactac attcttcaac gtcagcgcgc agaacaacga caccgttcac gccgtggtca
      661 cgcagggacg cgcactcgca tacggcagcg ccatcaacaa cgcctccggt gatcccacct
      721 acgtcccggg cctgctcgtc catcccgatc tgcgcatcaa cttcgttggc gtctcattcg
      781 gcaatgccgg agcgatcgac gtcgaggacg ccaatcacga cggcgtgctg gaccacgcaa
      841 tcgacatcca gaccggatcg tggcccatcg gcttccgcgt cgtcgtcaac tcgtcgacgg
      901 gcgcgacatt cgagctggtc gatcccccga gcgacgtgaa actcaccgac acgcacggcg
      961 gcatcctctg gaccccgagc gcatcgctat ccggcacgac cacaaccctc acgcttcgtg
     1021 tgaccgcaga tggctcgacg gagatcatca cgatcccggt caatcttcgg taagtctgag
     1081 tcttcttgat tcagggacga gggcatgtgc tctcgtccct ttttcttgat cagaaccgag
     1141 caccactgag acacagagga cacagagaat gctctgtgct ctctgtgtct cagtggttca
     1201 aaaagacttc acccgaacga gaccttcgcg aaccgccgct tccccacttt cagcacgtac
     1261 gactttccgc tcgaagcatc gagcgtcgcc cgcgggtcgt tcacctttgc atcgtcgacg
     1321 agcacgccgc cctgcgcgat cacacgctgc gcgtctttgt tcgattccga gagtccggcc
     1381 tggacgatga tcttcgacag caattgcggc tcgctcgcgg cgggcagcgt ggtctcctcg
     1441 atttccgccg gcgcctgcct ctcgctgaac acccgtctga actccgcctc ggcagcatcc
     1501 gcagccgccg catcgtggaa gtcgcgaatg atcgacttgg ccagttcgcg cttggcgtcc
     1561 atcggatgca gccctcccga ctcgaccgcg gcgcgcttct ccgcgatcgc cgcctgcgtc
     1621 agatcggtgc agagcgtgta gtacttccac atcagatcgt cggagatcga catcaccttc
     1681 ccgaagatcg atcccggatc ttcgttgatg ccgatgtagt tgccgagcga cttcgacatc
     1741 ttctcgatgc cgtcgagccc ttcgagcagc ggcatcgtga tgacgacctg cggctcgagg
     1801 ctgtactcgc gcatcaggtc gcggccgacg agcaggttga atagctgatc ggtcccgccc
     1861 atctcgacgt cgcacttgag cgccaccgag tcatagccct gcgccagcgg atacagcagc
     1921 tcgtgaatcg cgatcggctg atgcgcgtcg aagcgcttgc ggaagtcgtc ccgctccagg
     1981 atgcgcgcca gcgtgtactt ggccgccaga cggaccatcc cctcggcgcc aagcgcaccg
     2041 agccactcgg aattgaatcg gatctccgtc gtcttcggat cgaggatctt gaagacctgc
     2101 tgcttgtacg tctccgcgtt ctcgagcact tcctctttcg agagcgcggg ccgcgtcgcc
     2161 ttcttgccgg tcggatcgcc gatgagcccc gtgaaatcgc cgatcaggaa aatcacgcga
     2221 tgcccgagct gctggaagtg cttcatcttc cggatcacga ccgtgtgccc gagatgaatg
     2281 tcgggagcgg tgggatcgaa accgaccttg atcgtcagcg gtttcccgcg cccgagcttg
     2341 gtcttcagat ccttgcgttc gataaagtcg accgtcccct tgccgagaaa gtcgagttgt
     2401 tcgtcgagtg tcatggggcg cattgtaaag cgcggtctcg ggttgctggg tagtgggtca
     2461 attagaaaaa tcggcaacca cagaggcaca gagttcacag aggtccctct gtgttctctg
     2521 tgtctctgtg gttgcctttg tttttggcga ccacgaccca cgaccctgat tggtggaatt
     2581 tgacacagcc cgatcccgtc tctacactcg ccaacccgat gagcaccaac ttcgcccagc
     2641 agcaccatca tcaccacatc taggggtgcc gtgcgcgccc ccatcggacg aggggcgctc
     2701 agcgaaggga cgtagccaac tgacctgaac ctcttctccg atacgggaag aggtttttga
     2761 ctttatggcc acgaaatcga gatatccaac tggtgtgcgg cccttgctgg ccgaggagac
     2821 cgcgcgccgg cggcggatcg agttgcgctt cgtcgatgca ttgacgggtg ccggcttcag
     2881 cgaagtcgtg ttgccgatca tcgactacgt cgagccgtat gccgcgctcg tcgagcctgc
     2941 gacggcgaag cagagctatc gcttcgtcga ccgcgaaggc gacctcatcg ccatccgctc
     3001 cgacttcacg ccaatgctgg cccgcgcgct cgcaccgtcg atcgcgcccg aggagttgcc
     3061 gctgcgtctc ttctaccgcg gcgacgtcat tcgcgtcgaa gcctcccgcc tcggcgcgaa
     3121 ccgcgagttg ttccagatcg gagccgagat cgtcggcgat gactccatcg atgccgacgt
     3181 ggccatgctt cgcctggccg ccgatctcgt tcgcgctccc ggcgacgagc ctaccatcgt
     3241 gtacaacaac gtctcgatcg ctgcagcgct gatcgcgcag gcaggcgccg tgcgcgaagc
     3301 gcttgcggca aaacggatga ctgcgggagt cccggccgcg ctgcagacga tcgtcgcgaa
     3361 gctgatctcc ggcgaagcga cgctgaacga ggtcgcgccc ttcgctcccg aggcggcggc
     3421 cagactctcg gcgatcgaag aagcggtggc cggcgacgcg gtttttgcac tccatctcga
     3481 cgacatcgaa gacagtggct actacaccgg cctgcgcttt cgcgtctacg ccggaagcgc
     3541 gcgcacgaaa gttgcgcagg gcggacggta cgacaacctc tacgaacgtt tcggccagcg
     3601 cgccgccgcg atcggtttca cattcaccat cgacgatctg gattgaacga catgcgcatc
     3661 gcaataccca aaggccggct tcaggagcga gcgctcagcg tcttcgctga tgccggatat
     3721 gacatcccgt cagagacgga tctgaagacg aggaaactcg tcttcgaccg cggcggcatc
     3781 gaatggatct tcgtcaaaga cggcgacgtg ccggtctatg tcgagcatgg cgccgctgac
     3841 gccggcatcg ctggactcga tcagattctc gagcacgaat gcaccgccta ccagcccgtt
     3901 gagtttccgt tcggtcactg ccgcatgatg ctgatcgcgg caccgggcgc gccgccgctg
     3961 acgaaggtgg ccacgaagta cccgcgcatc acacgccagt tcctcgacca gcgcggcgtg
     4021 catgccgaga tcgttccgct gaccggctcc gtcgaactgg ccgccgtgct gaacctcacc
     4081 tcgcacgtcg tcgatctggt cgagactggc gagacggtgc gcattcacaa actggagatg
     4141 caggaagtcg tcgccgagat ctcgccacgg ctgatcgttg gaaagaactt ctatcgcacc
     4201 gaaccgaaga tggttcggga gctgatcgca aagattgagg aggcggcacg agtgtcatcg
     4261 tgaccggaaa gccaatgcga accttcacca caaacactcc cgaatccgat gaggcgctgc
     4321 gcgcgatcct cgagcgccgc agcgccacga acgagaacgc gctgcgcgtc gccgacgaga
     4381 tgatcgccgg ggtgcgcgcc cgtggcgatg cctatgtcgc cgaggtgatc gcgaagtttg
     4441 acggcgtcac actcgcgccg gatgcgatca ggatcgcgcc gcgtgacgtc acgatctccg
     4501 ccgagatgaa gaacgccatc gacctggcca tcgaccgcgt cgaagccttt caccggccgc
     4561 agcttccgca gtcctaccgc ctcggcaacc tcgagcaccg cgtccgcccc atccgccgcg
     4621 ccggcgtcta cgtgcccggc ggccgagcgg tctacctctc gaccttgatc atgaccgccg
     4681 ttcccgcgcg tatcgccggc gtcgaagaga tcgtttgcat cactacctca tccgccgcgg
     4741 accgtgatga gcttcactac gtctgcaacc gcctcggcgt acgcgacatc taccgctgcg
     4801 gcggcgcggc gggcgtcgcc gcggcagcga tcggcacgca gtcgctcgct cgtgtcgaca
     4861 agatcgtcgg ccctggcaac gcctacgtca ccgccgcgaa gatgcggctg atcggcgaag
     4921 tcgggatcga tatgaccgcc ggtccgaccg agttgatcgt catcgccgac gacatcgctc
     4981 accctcaatt cgtcatcgcc gatctactcg cgcaggcaga gcatggtgcc gattccgagg
     5041 tcgtgctctt gacgacgtcg gcgtggctgg ccgagcagat gcccgagcgc gcactctcgt
     5101 tcgtcgtcaa ctcgatggac gaggcaatcg ccattgccga ccgcatcgcg ccggagcatc
     5161 tgtcgattca cgcacgcgac gcggcatcga tcggcgcacg cgtgcagaac tgcggcgcga
     5221 tcttttgcgg tccgtccgcg ccacccgcgg ccggcgatta cgtcgtcggc acgaatcatg
     5281 tcctccccac cgccggcagc gcgcgcttct tctcccccct cggcgtctac gacttcgtca
     5341 agcgcgcgaa cgtcatctcc atcaccgccg aggagttggc cgaggtgagc gaagccggcg
     5401 aacggatcgc cacattcgaa ggattgcccg cccacgccgc atcgatggcc atcaggagga
     5461 ccgcatgtct cgcgacgtcg tagctgccat gaccgcctac acgctggagt tgcgtgaggc
     5521 ggcgatcaag ctgaatcaga acgagagtcc gaccgacttt ccgcaggagc tcaaagaaaa
     5581 ggtgctcgcg gagatggcag cgcgccactg gaacatctat cccgacttcg agtcgatgat
     5641 gctccgcggc gcgctggcga agacgcacgg atacgacacg gcgaacgtgc tcgtcggcaa
     5701 cggctcgaac gaattgcttg ccgccacgat cccgacattc atcggccccg gcacccccgt
     5761 cgtttttccg aagccgacgt ttgcactgta cgagaaactc gtcgcggtcg ccggcggcat
     5821 cgctctgccg atcgccgtcg atccgtcgac gggactctta ccgctcgacg agatgctcca
     5881 cgccatcgag cgctgcgaca acgccgtagt cattgtctgt tcgcccaaca atccgaccgg
     5941 aggcgtgctg cccgaaggcg gcgtcgacgc gctgctcgcc accggcgcga cggttctttt
     6001 cgaccgtgcg tacggcgact tcgccgaaga cacgatgccg ccgctgcacg accgcctcgt
     6061 cacgttttcg acgttctcga aagcctgggg cctggccgga ctgcgcgtcg gctggctggc
     6121 gtcgaccgct tccacctgcc gcgagatccg caaagtgaag ctcccctact cgctcaacat
     6181 catcagcgag gcagtcgccg caacggcgct cgagcacggc gagatccgca cacacaacgt
     6241 cgccgcaacg attgccgagc gcaagcggat gttcgaagcg atgcaggaga tcgaaggtgt
     6301 gacaccgttc ccgtcgcgat cgaacttcat cgccttcacc acgcagcgca atgccaagga
     6361 tgtcttcaac gcactgcacg cagacggcat cctcattcgc gacgtcagcg ccgcggtacc
     6421 gaatgcgctg cgagtgtcgg tcggcacgcc gtcagagaac gatgcgtttc tcgcagcgct
     6481 caaccaaacg atgaaggacg aacgaccatg agaacagcaa ccatcacccg caccaccaaa
     6541 gaaaccaacg tctccgtcac gctcgacctc gacggcgacg gcacccactc gaagatcgag
     6601 acgccggtcg ggtttctctc gcacatgctc gacgtcatcg cgcgccacgg ccgcgtcgat
     6661 ctcgacatcg tctccaccgg cgatacgcac atcgactatc accacaccgt cgaagacacc
     6721 gctctcgtcc tcggcgaagc cttcgcccag gccctcggcg ataagcgcgg catccgccgc
     6781 ttcggccacg cctacgtgcc gctggacgaa gcgctggcgc gcgtcgtcgt cgatttcgcc
     6841 ggccggccct ttctcgtctt cgacgttccc ttcgaccgcg agatgcttct catccacaaa
     6901 gatttcccct tcgcactcgt cgaggag
//
LOCUS       DATLVW010000228         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_297829, whole genome shotgun sequence.
ACCESSION   DATLVW010000228 DATLVW010000000
VERSION     DATLVW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297829"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1097
                     /locus_tag="VNN25_07365"
     CDS             273..1097
                     /locus_tag="VNN25_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91382.1"
                     /translation="MLIVPTLAANASVTGSLTANGKTVALKHAYAQTRKDPFDKTKSV
                     TQVIVTDQEISPAAASDDMELMQAQDKQQLSGFTATIDASKQVISATVFSPAFKKMKQ
                     FSGVGMQKAELTAMTATRIAGTISMAKPDDFFDEKYQYNATFDLPVGKPAGPPPPPAL
                     KGSPLPAGGGEPGKAYAVYLKNLTAGDMKAVLGGISADRAKQASADPDFKKLFPLLQE
                     MEPKGIKVTGGASDGTHATLLATGKEGDAVSHGTITMVRENGAWKVEKEDWQSKSE"
     gene            complement(1216..1521)
                     /locus_tag="VNN25_07370"
     CDS             complement(1216..1521)
                     /locus_tag="VNN25_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXH91383.1"
                     /translation="MCIPCTGGVPAVKGAELDALANSLGGGWRVVEEHHLEKEFTFPD
                     FAKALAFTNRVGAIAEAEGHHPDIYLAWGKVRITIWTHKVDGLTRADFVLAAKIDAG"
     gene            complement(1606..1803)
                     /locus_tag="VNN25_07375"
                     /pseudo
     CDS             complement(1606..1803)
                     /locus_tag="VNN25_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            1833..2606
                     /locus_tag="VNN25_07380"
     CDS             1833..2606
                     /locus_tag="VNN25_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH91384.1"
                     /translation="MNANTKTALDLTGKVVLITGGSRGIGAETARRMAAAGGHVIINY
                     RRSADEANALVQEIGGNAIAFCADVANPAALETMIDDVVAAFEKIDVLVNNAAVFEYN
                     PFDGDDYTAWQKGWRRTFDLNVFAAANASWLAMRAMRRSGGGKIINVASRAAFRGETE
                     FADYGASKAALVNLTRSIARACAKDNIVASCVAPGFIETEMAKPELEKNREEILRQIP
                     LGRVGTTADVANVILFLASSMADYLNGVTIDINGGSWFQ"
     gene            complement(2710..>3168)
                     /locus_tag="VNN25_07385"
     CDS             complement(2710..>3168)
                     /locus_tag="VNN25_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91385.1"
                     /translation="VAHHHDNERLIWLCDIHLLRERMSQEEHAAFWRLAAERRLVTIS
                     ERSIALAEDWFARISHDRAADWLRDDERLRDEPSAAFLDRSRSRAALLGGDLKALGWR
                     ARMRRLRELAFPPAAFMRQSFPSAPAVLLPALYLWRGVRGVLRLFRRVAG"
BASE COUNT          694 a         1066 c          889 g          519 t
ORIGIN      
        1 cgtcggtgta ctgcggcttc gtctgagtcg ttgtctgagc gtttgtctgg gcaggagccg
       61 tcaccgccgc gagaccaagc cacgccgcaa ggagaacgtt tcgcatagcg aaaatatacg
      121 ccgggagagg attcagcgcg aacgctgttg atctgcatca caaaactccc aaacccgatg
      181 ccgtatcttg cccacctctc tcgtatccaa cacacaaccg gaggtcatcg agcatgcatc
      241 gtttccgctc atccctgggt tccatcttcc tgatgctcat cgttcccaca ctcgccgcca
      301 acgccagcgt caccggctcg ctcaccgcga acggcaaaac cgtcgcgctc aagcacgcct
      361 acgcgcaaac gcgcaaggat ccgttcgaca agacgaagtc cgtcacgcaa gtcatcgtca
      421 ccgatcagga aatctctccc gccgctgcat ccgacgacat ggagctgatg caggcgcagg
      481 acaagcagca gctcagcgga ttcaccgcca cgatcgacgc cagcaaacag gtcatcagcg
      541 ccacggtctt cagccctgca ttcaagaaga tgaagcagtt ctccggcgtc ggcatgcaga
      601 aagccgaatt gacggcgatg acagcgacgc gcatcgccgg caccatttcc atggccaagc
      661 ctgacgattt cttcgacgag aagtatcagt acaacgctac gttcgatctc cccgtcggca
      721 aaccggccgg gccgcctccc ccacctgcac tcaagggatc accgcttccc gccgggggcg
      781 gcgagccggg caaagcctac gcggtctacc tcaagaacct taccgccggc gacatgaaag
      841 ccgtcctcgg cggcatcagc gccgaccgcg caaagcaggc atccgccgac cccgatttca
      901 agaagctctt tcccctgctt caggagatgg agcccaaggg catcaaagtc accggcggcg
      961 ccagcgacgg cacccacgcc acgctcctcg ccaccggcaa ggaaggcgac gccgtctccc
     1021 acggcacgat caccatggtc cgcgagaacg gcgcatggaa ggtggagaaa gaggactggc
     1081 agtcgaagag cgagtagggg cgatgtgcta gttgcttatc gtggtcgcta aagcaaaggc
     1141 aaccacagag acacagagaa cacagaggtt tctctgtgac ctctgtgcct ctgtggtgac
     1201 cggtttccta aacccctatc ccgcatcgat cttcgcagcc agcacgaaat ctgctcgcgt
     1261 caatccatcg accttgtgcg tccagatcgt gatccgcacc ttgccccacg ccaggtaaat
     1321 atcgggatga tgcccctccg cctccgcaat cgcgccgacg cgattcgtga acgccagcgc
     1381 cttcgcaaaa tcgggaaacg tgaattcctt ttccagatga tgctcttcga cgacgcgcca
     1441 tccgccgccg agtgagtttg caagcgcatc cagctctgca cccttcacag caggcacgcc
     1501 gccggtgcac ggaatgcaca tctcgtccgc cagcgacgag cgtgatgccg gcgcgctgga
     1561 atcgcgcagg taggccgcaa acgcacccgg ggtgacgcga tgccggaagg cttccacgcc
     1621 gtcgacatag atcaccggca catcatccgt gagcctcgcc cgcaacgccg cgtcggcatc
     1681 gatatcggtc accgttaccg tcaccggaag ctggtgcaac gcgatcgctt cgcggacagc
     1741 ggcataggcc ttctcgcaca gcggacagtc ggcgcgcgtg taaaacgtca cttcatgatt
     1801 catcgcataa actccgatgc cgaggatacc tgatgaacgc gaatacgaag acggcactcg
     1861 atctgacagg caaagtagtc ctgatcaccg gcggctcccg cggcatcggc gccgagactg
     1921 cgcggcgcat ggccgctgcc ggcggacacg tcatcatcaa ctaccggcgc agcgcagatg
     1981 aggccaacgc actcgtgcag gagatcggcg ggaacgcaat cgcattctgc gccgatgtcg
     2041 ccaaccctgc ggcgctcgag acgatgatcg acgacgttgt agcagccttt gaaaagatcg
     2101 atgtgctcgt caacaacgca gcggtgttcg agtacaaccc cttcgacggc gacgactaca
     2161 cagcctggca aaaaggctgg cgccggacgt tcgatctgaa tgtcttcgcc gccgcgaatg
     2221 cctcgtggct ggccatgcgc gccatgcgcc gcagcggtgg cggaaagatc atcaacgtcg
     2281 cctcgcgcgc tgcttttcgc ggtgagaccg aattcgccga ctacggtgcg tcgaaagccg
     2341 cgctcgtcaa cctcacccgc tccatcgctc gcgcctgcgc gaaggacaac atcgtcgcca
     2401 gttgcgtcgc gcccggattc atcgagacgg agatggcgaa gccggaactg gagaagaatc
     2461 gcgaagagat cctccgccag atccccctcg gacgcgtcgg caccaccgcc gacgtcgcca
     2521 acgtcatcct cttcctcgcc tcctcaatgg ccgactatct caacggcgtg acgatcgata
     2581 tcaatggcgg ttcgtggttt cagtgagccc caaaaaccat tgcaccacag agacacagag
     2641 atcacagagt caagcgattc aactctgtga cctctgtgtc tctgtggtta aagagtcttt
     2701 taagaaaact cagccggcca ccctccggaa caaccgcaac accccccgca caccgcgcca
     2761 cagataaaga gccggcaaaa gcaccgcagg cgcagacgga aaactctgcc gcatgaacgc
     2821 ggccggagga aacgccagct cgcgcaaccg gcgcatgcgt gcacgccagc cgagcgcttt
     2881 gagatcgccg ccgaggagcg cggcgcggct tcgggagcgg tcgaggaagg ctgccgaggg
     2941 ctcatcgcgg agacgctcgt cgtcacggag ccagtccgcc gccctgtcat gcgagatgcg
     3001 tgcgaaccaa tcctcggcca gcgcaatcga gcgctcgctg atcgtgacca aacggcgctc
     3061 ggcggccagt cgccagaacg cagcatgctc ctcttgcgac atccgctcgc gcagcaggtg
     3121 tatgtcgcag agccagatca aacgctcgtt gtcgtgatga tgcgcgac
//
LOCUS       DATLVW010000229         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_379765, whole genome shotgun sequence.
ACCESSION   DATLVW010000229 DATLVW010000000
VERSION     DATLVW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379765"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(772..2475)
                     /locus_tag="VNN25_07390"
     CDS             complement(772..2475)
                     /locus_tag="VNN25_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXH91386.1"
                     /translation="MTSNSARAPASPERRDFIREIVAADVREGRVSEVVTRFPPEPNG
                     YLHLGHSKAIALNFGIAQEFGGRCHLRFDDTNPIGEEQEYIDSIQEDIRWLGFDWGEH
                     LYFASDYFHQLYDWAVHLIKAGKAYVDDLSADKIREHRGTLTEPGKNSPWRDRSIDEN
                     LNLFERMRAGEFPDGARVLRAKIDMASPNINMRDPVLYRILHAIHPRTGDEWCIYPLY
                     DFAHGQSDAIEGITHSLCTLEFEDHRPLYDWLLENLPVPSRPHQYEFARLNFTYTVLS
                     KRFLGRLVSEGHVRGWDDPRMPTLSALRRRGFPPEGLRNFLDLVSLGGKGTSAAEIEM
                     LEHEVRDVLNRRALRRFAVLRPLRLVIENYPEGQVEEVEAVNNPEDPSAGTRKVPFAR
                     ELWIERDDFMEDPPRKFFRLAPGREVRLRYAYFVTCTDVVKDASGEIVELRCTYDPET
                     HGGDAPDGRRPKATLHWLSSEHAVPAQVRIYDRLFTRPDPGADGDLYADLNPNSETVL
                     QECLVEPALTELPVGETVQFERLGYFCPDPDSTPGRLVFNRTLGLRDTWAKLQAQAAR
                     P"
BASE COUNT          423 a          869 c          954 g          490 t
ORIGIN      
        1 actgtgcttc cctgatgatt aagcgtcgtg ctgcggctgc cgcggtccgg ccctgcgtgc
       61 tgccccgatc ccggctcagc gccctcgtcg tttccgcagt tcacacgcct ggaccggccg
      121 gacgggccgg cgctgccgcc tctttacgct tgcgaaatgc gggcccggcc ggcccttgtc
      181 gtgttagctt ggactggtga tggatcaccg atctggtgat gtatcactcg aatgggtgtt
      241 caagtccccc ctcggacaca tgggggttga agtccctagg gacacaagcc ccgggattcg
      301 gtgggtgcct gcgagtaagg ccacgccacc gcgcgcgagc gcttctcgat ccccgcgcgg
      361 gcaatgcggt cacatcgacg tggcgagaat gtcgtcacga tcacctcgat acagccccgc
      421 tcaaccaact cggccagggc aatgatgcac agcgcccggg atcttcaaac ccggctccgc
      481 atcgacctcg ccatccagat cggcagtgcg attgagcctg tgccgcacgc ccacgcgcag
      541 gtaacggccg atcggcgtcc tggcgcaggt ccgggtctgc tgtgacaccc cacaagaagc
      601 ctcggtcgtg gacgcgctcg cttcgttcgg tgtggtgcac gcggacatgc ccttgacgct
      661 ggccaacatc tggctcgcca tccaggggcc ggcctgttcg gagggacctg cccgccagtg
      721 tttccccctg aaccgccggg gcctgaagag ccgcggcgct gctacaggtc gttatggcct
      781 ggcggcctgc gcctggagct tggcccaggt gtccctgagg ccgagggtgc ggttgaagac
      841 gagccgtccg ggcgtcgagt cgggatccgg gcagaagtag ccgaggcgct cgaactgcac
      901 ggtctcaccg accggcagtt ccgtgagcgc aggctcgacc aggcactcct ggagcaccgt
      961 ctccgagttg gggttgaggt cggcgtagag gtcgccgtca gcgccgggat cggggcgggt
     1021 gaagagacgg tcatagatac gcacctgggc gggcacggcg tgctcggacg agagccagtg
     1081 cagcgtcgcc ttcggccggc gcccgtcggg ggcgtcgccg ccgtgtgtct ccggatcgta
     1141 ggtgcagcgg agctcgacga tctcgccgct cgcgtccttg accacgtcgg tgcaggtcac
     1201 gaagtaggcg tagcgcaaac gcacttcgcg gccgggggcg aggcggaaga acttgcgtgg
     1261 cgggtcctcc atgaagtcgt cgcgttcgat ccagagctcg cgcgcgaagg gcaccttgcg
     1321 tgtcccggcc gagggatcct ccgggttgtt gacggcctcc acctcctcga cctgcccctc
     1381 tgggtagttc tcgatcacga gcctgagcgg gcgcaggacg gcgaagcgtc gcagcgccct
     1441 ccggttcagc acgtccctga cctcgtgctc gagcatctcg atttcggcgg cgctcgtgcc
     1501 cttgcctccc agcgagacca ggtcgaggaa gttgcgcaga ccctcgggtg ggaagccgcg
     1561 ccggcgcagg gccgagaggg tgggcatgcg cggatcgtcc cagcctcgca cgtggccctc
     1621 gctcacaagc cgccctagga agcgcttcga gagcacggtg tacgtgaagt tgaggcgggc
     1681 gaactcgtat tggtgcgggc gcgaaggcac cggcaggttc tcgagcagcc agtcgtagag
     1741 cggtcggtga tcctcgaact cgagcgtgca gagggagtgg gtgatgccct cgattgcgtc
     1801 cgactggccg tgggcgaagt cgtacagcgg atagatgcac cactcgtcgc cggtccgcgg
     1861 gtggattgcg tgcaggatcc gatagagcac cgggtcgcgc atgttgatgt tgggcgaggc
     1921 catgtcgatc tttgccctga gcactctggc accgtccgga aactcccccg cgcgcatgcg
     1981 ctcgaacagg ttgaggttct catcgatcga acggtcgcgc caggggctgt tcttgcccgg
     2041 ctctgtgagg gtgccgcggt gctcccggat cttgtcggcg gagaggtcgt cgacgtaggc
     2101 cttgccggcc ttgatcaggt gtacggccca gtcgtagagc tggtggaaat agtcggaggc
     2161 gaagtagaga tgctcgcccc agtcgaagcc gagccagcgg atgtcttcct gaatcgagtc
     2221 gatgtactcc tgctcttccc cgatcgggtt ggtgtcgtcg aagcgcaagt ggcagcggcc
     2281 gccgaactcc tgggcgatcc cgaagttgag cgcgatcgcc ttggagtggc cgaggtggag
     2341 gtatccgttc ggctcgggcg ggaagcgcgt cacgacctcc gagactctgc cctcgcgcac
     2401 gtcggcggcg acgatctcgc ggatgaaatc gcgtcgttcg ggagaggccg gagcgcgggc
     2461 gctgttgctc gtcatctcga tcagcgtagt gcagtacatc gccatgtctg gcgcagtcgg
     2521 tgcactcacg ctcaggtgac aaccccaccg cacacccgga agccgccggc atcatccggc
     2581 aggcgagcgg cttccaacgc tttgatgaag cttgagcggg ccttgctcag cggccgggag
     2641 ccgtgagctg cgcgaagcag acgtcaggcg tttaggtcgt ggtctgcgtg gacccagctg
     2701 acgccatcaa ggtcgcggcg gatccggtcg ccgatc
//
LOCUS       DATLVW010000230         6304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_587665, whole genome shotgun sequence.
ACCESSION   DATLVW010000230 DATLVW010000000
VERSION     DATLVW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587665"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1082)
                     /gene="pheS"
                     /locus_tag="VNN25_07395"
     CDS             complement(24..1082)
                     /gene="pheS"
                     /locus_tag="VNN25_07395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXH91387.1"
                     /translation="MLDDITRISSEFDSALGSANTAAALDDLRVHYFGRKGGLIPALF
                     SRLKEVPKEQKKDAGDALNKLRDRLESELKEKSERVTTEESARKQSRDIIDVTLPARI
                     PHLGHLHPVTIIRREMETIFSEMGYSLDPGPEVESDWYNFEALNFTPDHPARDTQDTF
                     FLDADAPPRGEGSSRLPYLLRTHTSNVQIRSMENHTPPVKVLSCGRVYRRDEITMRRS
                     PMFHQAEGFLVDKGIHIGHLRATLEHFIKRLFGADSKMRMRPSFFPFTEPSAEVDMSC
                     VFCKGAGCGTCSQTGWMEILGCGMIHPQVLRNVGIDPEEWSGFAFGLGLDRCAMIKFD
                     IPNIRTLFENDLRVLQQF"
     gene            complement(1228..1584)
                     /gene="rplT"
                     /locus_tag="VNN25_07400"
                     /pseudo
     CDS             complement(1228..1584)
                     /gene="rplT"
                     /locus_tag="VNN25_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            complement(1724..1921)
                     /gene="rpmI"
                     /locus_tag="VNN25_07405"
     CDS             complement(1724..1921)
                     /gene="rpmI"
                     /locus_tag="VNN25_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXH91388.1"
                     /translation="MPKMKTHRGAAKRFKKSGTGKLQRNHAFHSHILTKKSAGRKAEL
                     GKETSVSKANEKTVKRMIPYL"
     gene            complement(2172..2729)
                     /gene="infC"
                     /locus_tag="VNN25_07410"
     CDS             complement(2172..2729)
                     /gene="infC"
                     /locus_tag="VNN25_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXH91389.1"
                     /translation="MFPPRRGSRFDRKPTEPESRINDMIRGIAEVRLIGADGSQIGVV
                     PLQQAQEMAREKDLDLVEISPTANPPVCKVMDYGKYVFQQKKKQTEAKKKQKVIVVKE
                     VQFRPRIDEHDFDFKKNNIIRFLQHGDKVKASIRFRGREMTHMELGRAVLDRLLLEIK
                     EYGAGENAHPDIQGNRMTMVISPVK"
     gene            complement(2785..4698)
                     /gene="thrS"
                     /locus_tag="VNN25_07415"
     CDS             complement(2785..4698)
                     /gene="thrS"
                     /locus_tag="VNN25_07415"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXH91390.1"
                     /translation="MHVQLPDGSQREVQDGATVADIALSIGKGLAKAALAGKVNGKVV
                     DVYTPVPDNAKIEIVTPKSEAGLDTIRHSTAHLMAMAVQELFPGTQVTIGPVIENGFF
                     YDFGTERPFTDDDLRRIEEKMSEIVKRDLPVRREELSRDEAIATFEKLGEKYKVEIIK
                     GIPGDEALSIYRQGEWFDLCRGPHVPSTGRLGAFKLTSVAGAYWRGDEHNAMLQRIYG
                     TAWADKDELSAYLKRVEEAHARDHRKLGKQLDLFTFSHFAPSMPIFLPKGAAIYNELI
                     DFVRGFYARDGYSEVVTPLIWDTEIFKISGHYENYKENMFFSEVDEREYGVKPMNCPG
                     HTQIFAMERRSYRDLPIRYADFARLHRYERSGVTHGLVRVRSFAQDDAHLFVTPDQIE
                     PEIDRELRLIKEIYDIFDFKEVRIALSTRPEKRLGTDEIWDNAEQGLENALKKNGVDY
                     HINPGEGAFYGPKLEFQVTDAIGRPWQLGTIQLDYALPERFKLSYIGADNGEHQPVMI
                     HRAILGSLERFIGIIIEHFAGAFPVWLAPVQASVLPLSEKFLDYGRETAGKLRAAGLR
                     IETDESNEKLGAKIRDAQLRKIPYMLVVGEKEVAAGTVSVRKRTGGDQVSLSIDEFVA
                     QTRAVIAARSLTL"
     gene            4976..6016
                     /locus_tag="VNN25_07420"
     CDS             4976..6016
                     /locus_tag="VNN25_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH91391.1"
                     /translation="MRTASIALTILLFAVAGGAQQPRILHATVTHVAASSDLGTQIRA
                     SKSAFIGYSVPEIESEHVMCCFNTYGDYRMGGTCSLDRDGSSFNSSDRDDLHVASSDV
                     FAIVYRVENGEIARVRSYSMDCALDANGAAVTWIDGVDPKKSVALQASMLGSSERRSG
                     SVMSTLAMHADASATTELERILNSSTAPDGTRGQAAFWLGQTRGRRGYEDVLAVAQSA
                     SASQHLREKAVFALSQSKEPGAIDALINLAKHDPIAHVRSQAIFWLSQKAGNKAAGAI
                     RASVDDDPDSAVRDKAVFAVSQLPNDESVPMLIELMKSHRDPSVRKKAAFWLGQKHDP
                     RALAAIEDVLKR"
BASE COUNT         1116 a         1797 c         2041 g         1350 t
ORIGIN      
        1 cctgttgaaa aagccgttag gcactagaac tgctgcaaaa cccgcagatc gttctcgaac
       61 agcgtgcgga tgtttgggat gtcgaacttg atcatggcgc agcggtctaa tcccaatccg
      121 aaggcgaagc cgctccactc ctcggggtcg atgccgacgt tgcgcaggac ctggggatgg
      181 atcatgccgc agccgaggat ctccatccag ccggtttgcg agcaggtgcc gcagccggcg
      241 cctttgcaga agacgcacga catgtcgact tcggcagacg gctcggtgaa ggggaagaag
      301 ctggggcgca tgcgcatctt ggagtcggcg ccgaagaggc gtttgatgaa gtgctcgagg
      361 gtggcgcgga ggtggccgat gtggatccct ttgtcgacca gaaagccttc cgcctggtgg
      421 aacatcggcg agcggcgcat ggtgatctcg tcgcggcggt agacgcggcc gcaggagagg
      481 accttcaccg gcggcgtgtg attctccatc gaacggatct gcacgttgga tgtgtgcgtg
      541 cgcagcaggt agggaagccg gcttgagcct tcgccgcgtg gtggcgcatc agcgtcaagg
      601 aaaaacgtgt cctgcgtgtc gcgcgcggga tggtcaggcg tgaagttgag cgcctcgaag
      661 ttgtaccagt cgctctcgac ctcggggccg ggatcgagcg aatagcccat ctcgctgaag
      721 atggtctcca tctcgcggcg aatgatcgtg acggggtgaa ggtgcccgag gtgggggatg
      781 cgggcgggga gggtgacgtc gatgatgtcg cgcgactgct tgcgcgctga ctcctcggtg
      841 gtgacccgct ccgacttttc cttcagctcc gattcgagac ggtcgcgcaa cttgttgagc
      901 gcgtcgccgg cgtccttctt ctgctctttc ggcacttctt tgaggcgcga gaagagggcc
      961 gggatcaagc cgcctttgcg gccgaagtag tggacgcgaa gatcatcgag agccgcggcg
     1021 gtgttggcgc tgccgagggc ggagtcgaat tcggagctga tgcgtgtgat gtcgtcgagc
     1081 atggcgagcg ggggattgta caaacaaaaa cgcgggcgca tggcccgcgt tttcaggtga
     1141 atcagtgatg gcgttttatg cggaggcctg ctttgcggcc gggcgcttgg ccgcggtctc
     1201 tttgtcgagg gcggtgcgaa gctgctgctt gttcagcgca cctttggcgc tttcgacgat
     1261 ggcggtgaag gcggcggcat cgcggacggc gatgtcggcc aggatcttcc gatcgacgag
     1321 cgagccggcg gctttgagtc cggccatgag cttcgaatag gaaatcccgt tgaggcgggc
     1381 ggcggcgttg attcgggcga tccagagctt ccggaaatca cctttgcggt ctttgcggcc
     1441 tgcgaaggcg gagttgagcg ctttctcaac ctgctgtttc gcgatccggt gaccgcgcga
     1501 cttcattccc cagtagccct tggcgagctt caggatcttg gagcggcggt ctttacgttt
     1561 ggtaccccgt ttgactctcg gcattgtttc tcctcgtttg gttcgttaag agcgggcggg
     1621 tcaggagtgg tgatttacca cgcctcgctc gggttggggt gtcgcttgtc gggtgtcggc
     1681 tttcggagca aaccgggaag cggaacaacc ggcagatgac agcctagagg tacgggatca
     1741 tccgcttcac ggttttctcg ttcgccttcg acacgctcgt ttccttcccc agctccgctt
     1801 tgcggccagc ggacttcttg gtgaggatgt gcgaatggaa ggcgtgattc cgttgaagct
     1861 tgcccgttcc ggacttcttg aaacgtttgg cggcgcctcg gtgcgttttc atcttcggca
     1921 tagcttggtc cctaacttag tgcggtggta ttgaattcct tgcgtggggt gtggggagta
     1981 gggggtgggg tgtgggagcg gaatcgacgt gcaatcctgc ttccctctcc gttctaccac
     2041 tcctgaatga gcttaagttc tgttgagact tgcgactttc gttttggcga ggtgtgtgac
     2101 gttggcagcg atggagaaag agcagctggt tttgattctg ctcccactcc ccacccccga
     2161 caccccacac cctacttcac cggcgatatc accatcgtca tgcggtttcc ctgaatgtcg
     2221 ggatgcgcat tttcgccggc gccgtattct ttgatctcga gcaggaggcg gtcgaggact
     2281 gcgcggccga gctccatgtg cgtcatctcg cgtcctcgga accggatcga tgccttgacc
     2341 ttgtcgccgt gctgcaggaa acggatgatg ttgttcttct tgaaatcgaa atcgtgctcg
     2401 tcgatgcggg ggcggaactg cacctccttg acgacgatca ccttctgttt cttcttggcc
     2461 tcggtctgtt tcttcttctg ctggaagacg tacttgccgt aatccatgac cttgcagacg
     2521 ggaggatttg ccgtcggcga gatctcgacg aggtcgaggt ccttctctcg ggccatttcc
     2581 tgcgcctgct gcaggggaac gacaccgatc tgcgatccat cggctccgat gaggcgaacc
     2641 tcagcgatgc cgcggatcat gtcattgatt ctggactcag gctctgtggg tttccggtcg
     2701 aatcgcgatc ctcgtcgtgg tggaaaaatg cgaaatcctc ctgttagccg ttagcgcgga
     2761 agcgcgaaat ctacgaataa aacactacaa agtcaacgag cgggcagcga tgaccgctct
     2821 ggtttgagcc acgaattcat cgatcgaaag cgagacctga tcgcccccgg tccgcttccg
     2881 gaccgacacc gttcctgccg ccacttcctt ctcaccgacc accagcatgt acgggatttt
     2941 ccggagctgc gcgtcgcgga tcttcgcgcc gagcttctcg ttcgattcgt cggtctcgat
     3001 gcgaagaccg gctgcccgta gtttccccgc cgtctcccgt ccgtagtcga ggaacttctc
     3061 cgacaacggc agcaccgatg cctgaaccgg tgcgagccaa acagggaagg caccggcaaa
     3121 gtgttcgatg atgatgccga tgaaccgctc cagcgagccg agtattgcgc ggtggatcat
     3181 caccggctga tgctccccgt tgtcggcgcc gatgtagctg agcttgaagc gctcgggcag
     3241 cgcgtagtcg agctggattg tgccgagctg ccagggacgg ccgatggcgt cggtgacctg
     3301 gaactccagc ttggggccgt agaacgctcc ctcgcccgga ttgatgtggt aatcgacgcc
     3361 attcttcttc aaggcgttct cgagtccttg ctccgcgttg tcccagatct catcggtgcc
     3421 gaggcgtttc tcgggacggg tggagagggc gatgcgcacc tccttgaagt cgaagatgtc
     3481 gtagatctct ttgatgagac gaagctcgcg gtcgatctcc ggctcgatct gatcgggagt
     3541 gacgaagaga tgagcgtcgt cctgcgcgaa cgagcggacg cgcacgaggc cgtgggtgac
     3601 accggagcgc tcgtagcggt gcagacgcgc gaagtcggcg tagcggatgg gcaggtcgcg
     3661 gtacgagcgg cgctccatcg cgaagatctg ggtgtggccg ggacagttca tcggcttcac
     3721 accgtactcc cgctcatcga cctcggagaa gaacatgttc tctttgtagt tctcgtagtg
     3781 gccggagatc ttgaagatct cggtatccca gatcagaggc gtgacgactt cgctgtaccc
     3841 atcgcgcgcg tagaagccgc gcacgaagtc gatcagctcg ttgtagatgg ccgcgccctt
     3901 cggcaggaag atcggcatcg acggcgcgaa gtgcgagaag gtgaagaggt cgagctgctt
     3961 gcccagcttt cggtgatcgc gcgcgtgagc ctcttcgacg cgtttcaagt acgccgacag
     4021 ctcgtccttg tccgcccatg cggtgccata gatgcgctgg agcatggcgt tgtgctcgtc
     4081 cccgcgccag tacgcaccgg cgaccgaggt gagcttgaac gcgccgaggc gaccggtgga
     4141 ggggacgtgg ggtccgcggc agagatcgaa ccattcgccc tggcgataaa tcgacagcgc
     4201 ctcatcgccg gggatgcctt tgatgatctc gaccttgtac ttctcgccga gcttctcgaa
     4261 cgtggcgatc gcttcgtcgc ggctcagctc ctcgcggcgc accggcagat cgcgtttgac
     4321 gatctcggac atcttctcct cgatacgccg gagatcgtcg tcggtgaagg gccgttcggt
     4381 gccgaagtcg tagaagaagc cgttctcgat gaccggtccg atggtgacct gggttccggg
     4441 aaagagctcc tgcacggcca tggccatgag atgggcggtg gagtggcgga tggtatcgag
     4501 accggcctcg ctctttggcg tgacgatctc gatcttcgcg ttgtcgggaa cgggcgtgta
     4561 gacgtcgacg accttgccat tgaccttgcc ggcaagggca gccttcgcca atcctttgcc
     4621 gatcgacagg gcgatgtcgg cgacagtggc gccgtcctga acttctcgtt gggaaccgtc
     4681 gggtagttgt acgtgcatgg ttttctccgg ggatggaaac gtaggtcgga ctctctagtc
     4741 cgacggtttt ttctcagtcg gactagagag tccgacctac gtttctcact cccccataaa
     4801 tcaaaagggc cgattgctcg gccctgtcgg attgtcgtgt tgcttgaaac agacgatcat
     4861 cggcagggcg ggttgaagca gcccgtcgtt cgcgtgttgc gccgtgtggt cgcccgtctc
     4921 ataaaagtcc gcggagtata gctaaccggc tcgaagcgcg ccagtctaag cgtgtatgag
     4981 aaccgcctcg atcgctctga caatccttct tttcgcagtc gcaggtggtg cgcagcaacc
     5041 gcgcatcctg cacgccaccg tcacccacgt tgccgcctcc tctgacctcg gcacacaaat
     5101 ccgggcctcg aaatcggcct tcatcggcta ctccgtgccg gagatcgaga gcgagcacgt
     5161 catgtgctgc ttcaacacgt acggtgacta ccgaatgggc ggtacgtgca gcctcgaccg
     5221 cgatgggagc tcgttcaaca gcagcgatcg ggacgacctg cacgtcgcct cctccgacgt
     5281 cttcgcgatc gtctaccgcg tcgagaatgg cgagatcgcg cgcgtgcgca gctattcgat
     5341 ggactgcgct ctcgacgcca acggtgcggc ggtgacgtgg atcgatgggg tggacccgaa
     5401 gaagagtgtg gccctgcagg cgtcgatgct gggaagctcg gaacgccgtt cgggttccgt
     5461 gatgtcaacg ctggcgatgc atgcagatgc ctcggcgacc acggagctcg aacggattct
     5521 gaattcgagc accgctcccg atggaacgcg cggtcaggca gccttctggc tcggacagac
     5581 gcgcggacgc cgcggatatg aagacgttct tgccgtcgcg cagtcggcct cggcctcgca
     5641 acacttgcgc gaaaaggcgg tcttcgctct gtcgcaatcg aaagagccgg gcgcgatcga
     5701 cgcgctgatc aatctggcga aacacgatcc gatcgcgcac gtgcgcagcc aggcgatctt
     5761 ctggctctcg cagaaagccg gaaataaagc cgccggcgcc atccgcgcgt ccgtggacga
     5821 tgatcccgac tcagcagtgc gcgataaggc ggtctttgcc gtgtcgcagc ttccgaacga
     5881 cgaatcggtt ccgatgctga tcgagctgat gaagtcgcat cgcgatccga gcgtgcggaa
     5941 gaaggcggcc ttctggctgg gacagaagca cgacccgcgc gcgctggcag cgatcgagga
     6001 tgtgttgaag cggtaaactc cacatctggc tatgcgcaaa acaaaggccg ctacttctca
     6061 ggggagaaca gttttcgctc gggcgcgaga gacaacttcg caggaagggc taatcgatga
     6121 gcgagtcatt gccgaagcgc atgacgactc ggcttgggaa aaactgattg aggtgaaacg
     6181 ccagcatccg gcggccattt ctctgatcgt aatcaagagg tgaaacaggc cgcgccactc
     6241 aagcgcgttc ttccttccgt tgcggcgaac acttcgcgcc ttcgcgtctt cgcgtcttcg
     6301 cgtg
//
LOCUS       DATLVW010000231         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_587761, whole genome shotgun sequence.
ACCESSION   DATLVW010000231 DATLVW010000000
VERSION     DATLVW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587761"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VNN25_07425"
     CDS             <1..489
                     /locus_tag="VNN25_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91392.1"
                     /translation="WIELSSTAPLTLPARMEAIVDSKPSIHPDARTRTLLYVEDNPAN
                     LKLIEKLVARRHDIRLLSAIDGTRGVELARTCQPDVILMDINLPGISGIDALKILRED
                     KSTSHIPVVALSANAIPRDIQKGLEAGFFRYLTKPIKVADFTDALNAAMEFADEGHVL
                     VH"
     gene            473..913
                     /locus_tag="VNN25_07430"
     CDS             473..913
                     /locus_tag="VNN25_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91393.1"
                     /translation="MSSSTNAFAARILIVDDQESNVRLLEFALRRGGYLAVTSTMRGD
                     RVCELHREQPYDLILLDLQMPRMNGFEVLAALALEAPVAVLVLSADPRQEAQSLAAGA
                     TGFLSKPFVLTEILARVEALLTADKLLPILVPTPATPVPLHVCL"
     gene            complement(988..2904)
                     /locus_tag="VNN25_07435"
     CDS             complement(988..2904)
                     /locus_tag="VNN25_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXH91394.1"
                     /translation="MDRREWLEANGIGGYVSSTVCGINTRRYHGLLIAATKPPVGRVV
                     LLSKLDERITIGRSSIDLGANQFPGAVHPRGFKHLLSFERGLFPTFEYAAEGVRIRKT
                     IAAIHGENTTVIVYELLESAVDVTLELRPFIAGRDYHSLVHANDAIHRDAAFDDGVFA
                     LQPYDGLPTLFLSVPDAAFLPAPDWYFNFEYAIEQERGLEAHEDLFTPGTFTRVLAKG
                     AALVVIASTSDPRGRDGLALFDIEHRRRESIANAAPMPGRFGRALTLAADQFIVRRGT
                     DRKTIIAGYHWFTDWGRDTMIALPGLCLTTGRFEDARSILLAFAECASEGMLPNCFPD
                     GGQAPEYNTVDATLWFFIAAQRYLESSGDEAFVRDTLLPVLRDIVAWHERGTRHNIHL
                     DDDGLLIAGSEGDQLTWMDVKIGDWVVTPRHGKAVEVNALWFNALTILADFERRWGAA
                     DASRTLVDSAERVRARFREVFWNEKNGSLYDVVRPDERDASIRPNQIFAISLPHPLLD
                     GADAEKVLRIVEQHLLTPRGLRSLSPEDPRFIGTYIGGPRERDAAYHQGTVWSWLLGP
                     YITALVRVRGEAGIVEGQRILKAFQPHLEESCIGTISEIFDGSPPHAPRGCAAQAWSV
                     GEVLRAAVEMGGQV"
     gene            3469..>3930
                     /locus_tag="VNN25_07440"
     CDS             3469..>3930
                     /locus_tag="VNN25_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH91395.1"
                     /translation="MEVREIQNGLRRSREQFIVRQQWATRPKDLRRALLDYRPTYVHF
                     CGHGAGEEGLILEEHFASAEALAGLFKLFADTIHCVVLNACYSTIQAMAIAEHIDYVV
                     GMNRDIGDSAAIVFAAAFYDALGAGESVEFAFELGRNAIQMVGLPEHLTPEL"
BASE COUNT          783 a         1272 c         1164 g          711 t
ORIGIN      
        1 tggatcgagc tctcctcgac cgcgccgctc acgctccccg cacggatgga agccatcgtc
       61 gattccaaac cttcgatcca tccggacgcg cggacgcgga cgctgctcta tgtcgaagac
      121 aatcctgcca atctcaagct gatcgagaag ctcgtcgccc gtcgtcacga tatccggctc
      181 ctcagcgcga tcgatggcac ccgcggcgtc gagcttgcgc gcacctgcca gcccgacgtg
      241 atcctgatgg acatcaacct tcccggcatc agcggcatcg atgcgctgaa gatcctgcgc
      301 gaagacaaga gcacatcgca cattccggtc gtcgccctca gcgccaacgc catcccgcgc
      361 gatatccaga aaggcctcga agccggcttc ttccgctacc tcacgaaacc gatcaaagtc
      421 gccgacttca ccgacgcgct gaatgccgcg atggaattcg cagacgaggg ccatgtcctc
      481 gtccactaac gcgttcgccg ccagaatcct gatcgtcgac gatcaggaat cgaacgtccg
      541 cctgctcgaa ttcgcgctgc gccgcggcgg ctatctcgcc gtaacctcga cgatgcgggg
      601 cgaccgcgtg tgcgaactgc accgcgagca accctacgac ctcatcctgc ttgacctgca
      661 gatgccccgc atgaacggct tcgaagtcct cgccgccctc gcgctcgaag cacccgtcgc
      721 agtcctcgtc ctctccgccg acccgaggca ggaggcgcaa tcgcttgcgg caggagcaac
      781 cggcttcctc agcaaaccct tcgtgctaac ggaaatcctc gcgcgcgtgg aggcattgct
      841 gacagcagac aagctgctgc cgattctggt gcccacgccc gcgacacctg tgccgttgca
      901 cgtgtgtttg tgagggtcag gtctaaagtc taaagtgtcg aggagacttt atcgctttag
      961 cgacgatcgt cctcggcact ttagacttta gacctgcccc cccatttcca ccgccgcacg
     1021 cagaacctct cccacgctcc acgcctgtgc cgcacaaccg cgcggggcgt gcgggggtga
     1081 gccgtcgaag atctccgaga tagtaccgat gcacgattct tcgaggtggg gctggaaggc
     1141 tttgaggatg cgctggcctt caacgattcc ggcttcgccg cgaacgcgaa cgagcgcggt
     1201 gatgtagggg ccgaggagcc aggaccagac ggttccttga tggtaggcgg catcgcgctc
     1261 gcgggggcct ccgatgtagg tgccgatgaa acgcggatcc tcgggcgaca gcgaccgcaa
     1321 tccccgcggc gtcagcaaat gctgctcgac gatgcgaaga accttctccg cgtcggcacc
     1381 gtcgaggaga ggatgcggaa ggctgatggc gaagatctga ttgggacgaa tactcgcgtc
     1441 gcgctcatcg ggtcgcacga cgtcgtaaag agaaccgttc ttctcgttcc agaagacttc
     1501 ccggaagcgc gcccgaacgc gctccgctga atcgacgagc gtacgggaag cgtccgccgc
     1561 tccccatcgc cgctcgaagt ccgcaaggat cgtcagcgcg ttgaaccaga gcgcgttgac
     1621 ctcgaccgct ttgccgtgcc gcggtgtgac tacccagtcg ccgatcttca catccatcca
     1681 ggtgagctga tcgccctcgc tgccagcgat cagtaagcca tcgtcatcga ggtggatgtt
     1741 gtgccgcgtt ccgcgctcat gccaggcaac gatgtcgcgc agcaccggca gcagggtgtc
     1801 gcgcacgaag gcctcgtcgc cgctgctttc gagataacgc tgcgcggcga tgaaaaacca
     1861 gagcgtggcg tcgaccgtgt tgtattcggg tgcctggccg ccatcgggaa agcagttcgg
     1921 cagcatcccc tcgctcgcac actcggcgaa cgcgagcagg atgctgcgcg cgtcttcaaa
     1981 gcggccggtg gtgaggcaga ggccgggcag cgcgatcatc gtgtcgcgcc cccaatcggt
     2041 gaaccagtga tagccggcga tgatcgtctt ccgatcggtt ccgcgccgga caatgaactg
     2101 atcagccgcg agagtcagcg cacggccgaa ccggcccggc atcggcgcag cgttcgcgat
     2161 cgattcacgg cggcggtgct caatgtcgaa cagcgccagt ccgtcgcgcc cccgaggatc
     2221 ggatgtcgat gcgatgacga cgagcgccgc gcctttcgcg agtacccgag tgaacgtccc
     2281 aggcgtgaag agatcctcgt gcgcttcgag ccctcgctcc tgctcgatcg cgtattcgaa
     2341 gttgaagtac cagtcgggcg caggaaggaa cgccgcgtcg ggcacgctca gaaagagtgt
     2401 cggaagcccg tcgtacggct gcagcgcgaa gacgccatcg tcgaacgcgg catcgcgatg
     2461 aatggcgtcg ttcgcgtgaa cgagcgagtg atagtcgcgt ccggcgatga acggccgcaa
     2521 ctccagcgtc acatccaccg ccgactccag caattcgtaa acgatcaccg tcgtgttctc
     2581 gccatgaatc gccgcgatcg tcttccgtat cctcaccccc tcggcggcat actcgaacgt
     2641 gggaaagagc ccccgctcga acgagagcag atgcttgaac ccgcgcggat ggacagcgcc
     2701 cggaaactgg ttcgcgccga ggtcgatcga cgatcgccca atcgtgatgc gctcatcgag
     2761 cttggaaagc agcaccaccc ggcccaccgg cggcttggtc gccgcaatga gcagaccatg
     2821 gtaacggcgc gtgttgatcc cgcaaacggt ggaactgacg tacccgccga ttccgttcgc
     2881 ctccagccat tcccggcgat ccatggttgc aaggatgcca cgggtgccca ccgggcgcga
     2941 aaccaagcgc gctcttgcat tacgagcaag cggcgaccgt tttggcgggc aaaactggaa
     3001 gcaagacgaa atcggaatga gtgaaagggg aagccgctga tgatcccaag aagacacaga
     3061 cgttgagcgc gaatctgacg ccgcgagagt ctttcgccag accgaacgtc acatgagcac
     3121 gcccacccaa tggcccgctt aatcggccct cgccacgttc atcggcgaag gttcatcgtc
     3181 cgccacctca gcaaaatgag ccgcatgacg atgacgttcg acgagattca ccatcggccg
     3241 atcagactcc cggtcgcgcc ctcgaccact tttttcttgg ttccagccat gccggggtag
     3301 ggaatactgt cgctgcgtca ctcctaacag cagggaggcg cttgcctagc ttggttcgat
     3361 aactcaagat tatcggcgtt cacaacattc gacatggctc cggacacgcc tcagacgatc
     3421 ctcatctgcg ccgccaatcg tagcgatcag gagcctctga gtctggatat ggaagtccga
     3481 gagatccaga atgggctgag gcgttctcgc gaacagttca tcgttcggca gcagtgggcc
     3541 acacgcccca aagaccttcg gcgagctctc ctcgactacc gtcctacgta cgttcatttt
     3601 tgcggccacg gcgccggcga ggaaggactg attcttgaag aacactttgc cagcgccgaa
     3661 gcgctagccg gactgttcaa gctttttgcc gacacgattc actgtgtcgt cctcaatgcc
     3721 tgctattcga cgattcaggc aatggcgatt gcagagcaca tcgattacgt cgtcgggatg
     3781 aatagggaca taggcgattc ggcggcaatc gtcttcgcag ccgcgttcta cgacgctctg
     3841 ggagcggggg aatccgtcga gttcgcgttt gagctcggtc gcaatgcaat ccagatggtg
     3901 ggtctcccgg aacatctcac gcctgagttg
//
LOCUS       DATLVW010000232         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_343989, whole genome shotgun sequence.
ACCESSION   DATLVW010000232 DATLVW010000000
VERSION     DATLVW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343989"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VNN25_07445"
     CDS             complement(<1..932)
                     /locus_tag="VNN25_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91396.1"
                     /translation="MAEWSIVITGPAGSAKFDPNPQPVSSNDIVFWSNRTDDPHTIVI
                     DGQPKVLPMPVPPWESSKPAYQVSAAVTYRCKDHDNETGSITIKTAVLLLALMTGFFA
                     PSLKAQVTCGQLIGGELKSPAEIGVHDATKRTTSGTLVTSGEKQLIGLPTGTRVTTGP
                     ITEANITCNAQWMRTYRNHEPAPDSQQPNAPAVLPEPGPTIRTHVGDLVELTFLNLID
                     DLNFPGTDTGDCDQVQGNNGQVYPQTDKMPNCFHGSVFTNVHYHGTHTSPNSTADNVF
                     LEIVPSPRSKNNARTPSITAASVKDQFAEFFGNC"
     gene            complement(1140..1499)
                     /locus_tag="VNN25_07450"
     CDS             complement(1140..1499)
                     /locus_tag="VNN25_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXH91397.1"
                     /translation="MIVEKKHAENFTILYVEGLIKLGESAEFFSAALENVLKNESANV
                     IIDFTKIDYIDSTGIGELVGYLGKFTSQNRKLILVNPSERILKLLKLAKLDSVFKIYN
                     TEEEAVSAESGNAAKSA"
     gene            complement(1977..2213)
                     /locus_tag="VNN25_07455"
     CDS             complement(1977..2213)
                     /locus_tag="VNN25_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXH91398.1"
                     /translation="MTRSEIEAKLSEIVRKEKDVADDKMTLETPLAEAGIDSLDALTI
                     LFAIEEEFHISIPDDRARAIRTFGDMVEVIEALV"
     gene            2235..2648
                     /locus_tag="VNN25_07460"
     CDS             2235..2648
                     /locus_tag="VNN25_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91399.1"
                     /translation="MFRRYWLLYPAWIVLCAVLFLASRHREDPSRRAGRMLSDDAAVR
                     AVAILRQRDPMHFQGYEAVHVAWATRGEGGGSTDRWVVLCDRVPHTALRDAVVVELDG
                     KDGRLLVIRKPAGRSESRDKGPESRDKGPEIRDNG"
     gene            2764..>5192
                     /gene="mutS"
                     /locus_tag="VNN25_07465"
     CDS             2764..>5192
                     /gene="mutS"
                     /locus_tag="VNN25_07465"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXH91400.1"
                     /translation="MSRPMKLTPMLEQYFEIKQQVPDAILFYRLGDFYEMFFEDAEKA
                     APLLDLVLTARNKGQEHEAPMCGVPYHSADGYIAKLIRHGLRVAICEQTEDATAAKGL
                     VKREIVRIVTPGTATESFIVDRESCYLVALFVAGVGSRESGVGDKAASTPTSALTPDR
                     RQPSPTAQETASAPTPDPRLPTPVGAAYLDVSTGDFFVTSYGGVDDPRLADDIARFAP
                     REAIVPRESAFPLEIPVTPVEPSMFETVAAHDYLSRHFGTQSLRGFGLEGDDPRIGAA
                     GAALRYAAASHKKPLDHVLGLRVDHEADFLQLDAATLANLEILDSRDAARPRASLWSV
                     VNATRSAMGGRMLRKWITRPLKSRDAIFDRHDAVDELTRSRAILEPMTQRLSRIADLE
                     RLASRVTLRTATPRECLTLADSLLMVDDLRTVMAPLHGPLLQRLRVALDPIAPVVDQI
                     AATLSDTPPLNMRDGGAIKPGVDADLDELRTIARDSKTILVEIEGRERERTGIGSLKI
                     RFNSVFGYYIEVSKSNLAKVPDDYIRKQTLVNAERYITPDLKVLEEKIAGAEEKAIAI
                     EARLYEALLESVARMSGAILATARAVGEVDALSSLATIAVRNRYVRATLSEGAEICVE
                     DGRHPVIEAIASERFIPNHTDVERDRNGIQIITGPNMGGKSTYLRQVALIVLLNQIGS
                     FVPASKARLGIFDRIFTRVGASDQLARGESTFMVEMHETANILNNASDRSLIILDEVG
                     RGTATFDGLSLAWAIIEYLHDNPTRGGITLFATHYHEVTDLAKTKTRVANYSVAVKEW
                     NEQIIFLRRVV"
BASE COUNT          935 a         1599 c         1632 g         1026 t
ORIGIN      
        1 tcgcagttgc cgaagaactc cgcgaactga tccttcacgc tcgcggccgt gatcgacggg
       61 gtacgggcat tgttcttcga ccgcggcgaa gggacgatct cgaggaacac attgtcggcc
      121 gtggaattgg gactggtgtg cgtgccgtga tagtgaacgt tcgtgaagac cgacccgtgg
      181 aagcagttgg gcatcttgtc ggtctgcggg tagacctgac cgttgttgcc ctgcacctgg
      241 tcgcagtcgc cggtgtcggt gccggggaaa ttgaggtcgt cgatcaggtt cagaaacgtc
      301 agctcgacga ggtcgcccac gtgggtgcga atcgtcgggc cgggctcggg aaggacagcc
      361 ggcgcgttgg gctgctggct atccggtgcc ggctcgtgat tccggtacgt ccgcatccat
      421 tgcgcgttac aggtgatgtt ggcctccgtg atcgggccgg tcgtcacgcg cgttcctgtt
      481 ggcaatccga tgagctgctt ctctccgctg gtgacgagcg ttccgctcgt cgtccgcttg
      541 gtcgcgtcgt ggacgcctat ctccgcgggt gacttcaact cgccgccgat gagttgcccg
      601 cacgtcactt gcgccttcag cgacggcgcg aagaagccgg tcatgagcgc cagcaggagc
      661 accgccgttt tgatcgtgat cgatcctgtt tcgttgtcgt ggtctttaca gcggtaggtg
      721 accgccgccg agacctggta ggccggcttc gacgattccc acggtggcac aggcataggc
      781 aatactttgg gttgcccgtc gatgacgatc gtgtgcggat cgtcggtacg gttcgaccaa
      841 aagacgatgt cgttcgaaga cactggttgt gggttgggat caaacttcgc tgaacccgcc
      901 ggacccgtga tgacgatgga ccattccgcc atgggaagcc tccttctctc tctcaatagt
      961 taggagtgac gaccgcagcg gtaaccctac ctcaaatcgg gtcgtaggga gtggggagtt
     1021 gggggtgggg ggagggagga acagagatca gggagcagag atcagagatc agggatctgg
     1081 agatcaggga tcagaaggcg gcctgacctc tgatctcttg tctcttatct ctgcctctct
     1141 cacgcgctct tcgcggcgtt gccgctttcc gcgctgaccg cctcttcctc ggtgttgtag
     1201 atcttgaaca cggaatcgag cttggcgagt ttgaggagct tgaggatccg ttcggagggg
     1261 ttgacgagga tgagcttgcg gttctggctc gtgaacttgc caaggtagcc gacgagctca
     1321 ccgatgccgg tggagtcgat gtagtcgatc ttggtgaagt cgatgatgac gttcgcgctc
     1381 tcgttcttga gcacgttctc gagcgcggcg gaaaagaact ccgcgctttc gccgagcttg
     1441 atcagccctt cgacataaag aatcgtgaag ttctcggcgt gcttcttttc gacgatcaat
     1501 gacggcctcc ggtgttcggg tgaagagtag caaatcaggt gccgggggtg ggggagctgg
     1561 ggagtgggtg gggacttgga gagcgatcac gcttcctcgg ccatctttgc cgcgaaggcc
     1621 tccagtcatt gtgcgaatcg cgcgaagtgt gaggtgtcat ccaaggtgtc atccaaggtg
     1681 tcatcctgag cctgccggag gcgcccgcgc aacacgttgc tctcgcgcag atcatcccag
     1741 gcgccgcagg acggcgaagg atctcaaaat gcgaagatct cgttcgtgcg tcttgaacac
     1801 gatcgctcgc gctggacgaa cacgatctgc gaagtctgag atccttcgac gtcctgcgcc
     1861 gccgagcaaa cgcagcgcaa acatcgcgat tcgcgcgggc ggctccggca gtctcaggat
     1921 gacacaacaa ccctccgaca tccgatctcc cactccccac tccccacccc ccaccctcac
     1981 accagcgcct caatcacctc caccatgtcg ccgaaggtgc ggatggcgcg ggctcggtcg
     2041 tcgggaatcg agatgtggaa ctcttcttcg atggcgaaga ggatcgtcag ggcgtcgagc
     2101 gagtcgatgc ctgcttcggc gaggggcgtt tccagagtca tcttgtcgtc cgcgacatcc
     2161 ttttctttgc ggacgatctc cgacagcttc gcttcgatct cggaacgcgt catcgggccg
     2221 ctatgatacc gcgcgtgttt cggcgctatt ggctgctcta ccccgcgtgg attgtgcttt
     2281 gcgcggtcct ctttctggcg tcgcggcacc gcgaagatcc ctcgcgccgc gccggccgca
     2341 tgctcagtga tgacgcggcc gtccgggccg tggcaatcct gcggcaacgt gatcccatgc
     2401 acttccaagg atacgaagcg gtgcacgttg cctgggccac gcgcggcgag ggcggcggca
     2461 gcaccgatcg atgggtcgtt ctctgcgacc gcgtcccgca caccgcgttg cgcgatgccg
     2521 tcgtggtgga gctggatggg aaggatggga ggttgctagt gattcgaaag cctgcaggga
     2581 gatcagagag cagagacaag ggaccagaga gcagagacaa gggaccagag atcagagaca
     2641 acggataaca acggatcaga tatcagagat cgggcaactc ggtcccccta gtctctgatc
     2701 tcttgtctct tgtctctgat gtcaacattc ttcatccttc acccttcata cttcacaact
     2761 ccaatgtccc gtcccatgaa gctcacgccg atgctcgagc agtacttcga gatcaagcag
     2821 caggtgcccg acgccatcct tttctatcgt ctcggcgatt tctacgagat gttcttcgag
     2881 gacgccgaga aggccgcgcc gcttctcgat ctcgtgctca cggcgcgaaa caaagggcag
     2941 gagcacgaag cgccgatgtg cggcgtgccg tatcactcgg ccgacggcta catcgccaag
     3001 ctgatccgcc atggcctgcg cgtggcgatc tgcgaacaga ccgaggatgc gacggcggcg
     3061 aaagggttgg tgaagcgcga gatcgtgcgc atcgtcacgc cgggcaccgc gacggagagt
     3121 ttcatcgttg atcgggagag ttgctatctg gtggcgttgt ttgtggcggg ggtcgggagt
     3181 cgggagtcgg gggtcggaga taaagcggct tcaacgccga cttcagcttt gacgcctgac
     3241 cgccgtcagc cttcgccgac tgctcaagag actgcctcag ctccgacccc cgacccccga
     3301 ctcccgaccc ccgtcggggc tgcctacctc gacgtctcca ccggcgattt cttcgttacc
     3361 tcgtatggcg gtgtcgacga cccgcggctt gcggatgaca tcgcgcgttt tgcgccgcgc
     3421 gaggcgatcg ttccgcgcga gtccgcgttc ccgctggaga ttccggtcac gccggtcgag
     3481 ccgtcgatgt tcgaaaccgt ggcggcgcac gattacctct cgcgccattt cgggacgcag
     3541 tcgttgcgcg gcttcggcct ggagggcgac gatccacgca tcggcgccgc cggcgcggca
     3601 ttgcgctacg ctgctgccag ccacaagaaa ccgctcgatc acgttctcgg cctacgcgtc
     3661 gatcacgaag ccgatttcct acagctcgat gccgccacgc tcgccaatct ggagatcctc
     3721 gactcccgcg atgccgcccg gccgcgcgcg tcgctgtgga gtgtcgtcaa cgccacgcgc
     3781 tcggcgatgg gcgggcggat gctgcgcaaa tggatcacgc gtccgctcaa gtcgcgcgac
     3841 gctatcttcg accgccacga tgccgtcgac gagctgaccc gctcgcgcgc cattctcgag
     3901 ccgatgacgc aacggctctc ccgcatcgcc gatctcgaac ggctggcttc gcgcgtgacg
     3961 ctgcggacgg ctacgccgcg cgagtgcctg acgctcgccg attcgctgct catggtcgac
     4021 gacttacgga cggtgatggc tccccttcat ggtccgttgc tccagaggtt gcgcgttgca
     4081 ctcgatccga tcgcgccagt cgtcgatcag attgcggcga cgctctccga tacgccgccg
     4141 ctcaacatgc gcgatggcgg cgcgatcaag ccgggtgtcg acgccgatct cgacgagctg
     4201 cgcacgatcg cccgcgactc gaagacgatc ctcgtcgaga tcgaagggcg ggaacgcgaa
     4261 cgaaccggca tcggctcgct gaagatccgc ttcaactccg tctttggcta ttacatcgag
     4321 gtctcgaagt cgaacctcgc caaggtgccg gacgattaca tccgcaagca gacgctggtc
     4381 aacgccgagc gctacatcac gcccgacctg aaagtgctgg aagagaagat cgccggcgcc
     4441 gaagagaaag cgatcgccat cgaggcgaga ctttacgaag cgctgctcga aagcgtggcg
     4501 cgcatgagcg gcgcaatcct cgccaccgct cgcgcagtcg gtgaggtcga tgcgctctcg
     4561 tcactggcca cgatcgccgt gcgcaaccgc tacgtccggg ctacgttgtc cgagggagcg
     4621 gagatttgtg ttgaggacgg aagacatcct gtcatcgagg ccatcgccag cgagcgcttc
     4681 atcccgaacc acaccgacgt ggagcgcgac cgcaacggaa tccagatcat caccggcccg
     4741 aacatgggcg ggaagtcgac gtatctgcgc caggtcgcgc tgatcgttct cctcaatcag
     4801 atcggcagct tcgttcccgc atccaaagcc cgcctcggca tcttcgaccg catcttcacg
     4861 cgcgtgggag cgtcggacca actggcgcgc ggcgaatcga cgttcatggt ggagatgcac
     4921 gagaccgcga acatcctgaa caacgcctcc gaccgatcgc tcatcatcct cgatgaagtg
     4981 ggccgcggta cggccacgtt cgacggcctt tcgctggcgt gggccatcat cgaatacctg
     5041 cacgacaatc ccacgcgcgg cggcatcacg ctcttcgcta cgcactacca cgaggtcacc
     5101 gacctggcga agacgaagac gcgcgtcgcg aattattcgg tggctgtgaa ggagtggaac
     5161 gagcagatca tctttctccg ccgcgtcgtc ga
//
LOCUS       DATLVW010000233         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_177596, whole genome shotgun sequence.
ACCESSION   DATLVW010000233 DATLVW010000000
VERSION     DATLVW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177596"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..1403)
                     /locus_tag="VNN25_07470"
     CDS             complement(477..1403)
                     /locus_tag="VNN25_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91401.1"
                     /translation="MFRCRFISLLLFLCTSSSLLAQLPANTILVKGAEPSASDTKTPL
                     PEAGKVDGGLYENRYFGFSYHLPPDWSEPYTGAPPSDSGSYVLAEFVPSKTFKGPING
                     TILITAQDLFFSLVPQRSAGEMIKYSSDHLQPYYTLEHPPSDLTIAGRSFARFDYMSP
                     VAGLHWYTLATQVRCHVIQFVLNSRDTGLLDSLVHDMDQMKLAANDSPPCVAGYAEQN
                     VISKVDPVLNDRKFNSIPVRIIIGTDGKVKHVHVISAFQDQAKIITDALFQWRFKPYQ
                     ENGKAVDVETGILFGYSPPRQQTAPTSQSTSQ"
     gene            complement(1471..2883)
                     /locus_tag="VNN25_07475"
     CDS             complement(1471..2883)
                     /locus_tag="VNN25_07475"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXH91402.1"
                     /translation="MAIRRVVPLAVFLSLLFVLQGFAQTDPGVRGGGAAAGSPLASVA
                     ANNPVTILNFFNDAKARFQEIDSVAGTIAGENGVGLGPRFNSRSCAACHAQPAVGGTA
                     PATNPQVADATADGAQNSVPSFITSTGPVREARFIFFTDSFGNPVTSQPNGGVEDLYT
                     ITGRSDAGPCSIGQPGFALQLSNNNVIFRIPTPTFGTGLIETISEETLLNNQQAAFNN
                     NQGITGTFNHNGNDGTISRFGWKAQNKSLELFAGEAYNVEQGVTNEIFTQERPLPGED
                     MGIIGGHSSTNGLPANCRGNATPEDHTNFNQTAVGTLSDLAQFAMFMRLLAPPTPSTT
                     VPGGSTSITNGRNLFISIGCAVCHTTSLTTGTSSFTSSLSNTPANLFSDLEIHHMGTT
                     LADNVSQGGAGGDQFRTAPLWGAGQRIFFLHDGRTKDIQAAILAHNANGSEANGPSFT
                     FFTNLSTGQRQDILNFLRSL"
     gene            complement(3377..>4026)
                     /locus_tag="VNN25_07480"
     CDS             complement(3377..>4026)
                     /locus_tag="VNN25_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91403.1"
                     /translation="ARAFAVGQAVAFSQSLDNLARHARGEKWPEFSDLSCINCHHSLE
                     GSGWRQERGWPGRAGLPAWSPQHWAVLRLLVGRANPSARAQLDNDVAIIATRVSHMND
                     SNGVLQTAVEARRVIDGVTPQIAALSWRDDDVRALMRTIAGDTDFILTSDVHSAEQTA
                     LALQSLASALTRSNPKLLKSPMTEAIDALFAEIQNRERYEPARFVQKLAALRAAL"
BASE COUNT          753 a         1137 c         1325 g          811 t
ORIGIN      
        1 gtccgcaaga tggacgccac gatcacgttc gtcggcggaa aggtggcgga gatcaacaca
       61 gcgaagtggg agaacgatta ggggggcgga gaacgaagcc cagcggcctc cgcggctccg
      121 cgtgaaccgg ttttctcttc ctattcacga gttccgacta ccgcacacgc gatttgactt
      181 cccactcacg gattccggtt ttccgttcac ggattcccgt ttaccgttca cggattcccg
      241 tttaccgtta acggattccc atttaccatt catggattcc tgtttaccgt taacggattc
      301 ccatttaccg ttcatggatt cctgtttacc gttaacggat tctcattttg tctaacttcc
      361 atttttggtg gtgtagttgt ccaccttttg tgacagtcgc tgccctgact cattccaccg
      421 gtgatccctt ctcgtctcga tatcctggtc aaccgagttg acatacgagt tgagggtcac
      481 tgcgacgtcg actgcgacgt cggcgccgtt tgctgcctag ggggcgaata gccgaacagg
      541 atgccggtct cgacgtcgac ggccttcccg ttctcctgat atggcttgaa cctccactgg
      601 aagagagcat ccgtgatgat cttggcctgg tcctgaaacg cactgatgac atgaacgtgc
      661 ttcaccttgc cgtctgtgcc gatgatgatg cggacgggga tggaattgaa cttgcggtcg
      721 ttgagtaccg gatcgacttt gctgatcacg ttctgctccg cgtagccagc cacgcacggg
      781 ggtgagtcgt tcgcggccag cttcatctga tccatgtcat gcacgaggct atcgagcagg
      841 ccggtgtcac ggctgttgag cacgaactgg atgacgtgac aacgaacctg cgtggccagg
      901 gtgtaccaat gcagacctgc caccggcgac atgtagtcga accgggcgaa cgaccggcct
      961 gcaatcgtca aatcggacgg cggatgctcg agggtgtagt acggctggag gtgatcgctg
     1021 gagtacttga tcatttcccc tgcgctgcgc tggggaacga gcgagaagaa aagatcctgc
     1081 gccgtgatga ggatcgttcc attgatcggg cccttgaacg tcttcgaagg tacgaattca
     1141 gcgagcacgt agctgccgct gtccgatggc ggagcaccgg tgtacggttc cgaccagtcg
     1201 ggcggcaggt gatacgaaaa gccgaagtat cgattctcgt agagaccgcc gtcgaccttg
     1261 cctgcttcgg gcagcggagt cttcgtatcg ctggcgctcg gctccgctcc cttgacgaga
     1321 atcgtatttg ccggcaactg cgcgagaagg gatgaggatg tgcagaggaa caaaagcaga
     1381 gagatgaacc ggcaacggaa cacgtgcacc tccttcgaga agccggccgc tgacgcgttt
     1441 gcgtcagcgg ccggcccggg acaacaccga ttagagagaa cgcaagaagt tgaggatgtc
     1501 ctgacgctgg ccggtactga ggttcgtgaa gaacgtaaag gacggcccgt ttgcttcgga
     1561 gccgttggca ttgtgggcca ggatggcggc ctggatatcc tttgtccgtc catcgtgcag
     1621 gaagaagatg cgctgtccgg ctccccatag gggtgctgtg cggaactgat cgccaccggc
     1681 gccgccctgc gagacgttgt cggcaagcgt ggttcccatg tggtgaatct cgagatcgct
     1741 gaagaggttt gcgggagtgt tgctcagact cgacgtgaat gaggaagttc cggtggtgag
     1801 gctcgtcgtg tggcagaccg cacagccgat ggagatgaag aggttgcggc cattggtgat
     1861 cgaggtcgat ccccccggca ccgttgtcga cggagtgggc ggcgccagca gacgcatgaa
     1921 catcgcaaac tgcgccaggt cgctgagcgt cccaaccgcg gtctgattga agttggtgtg
     1981 atcctccgga gtggcattgc cccggcagtt cgcgggcaaa ccattcgtcg atgagtgacc
     2041 gccgatgatg cccatgtctt ctccgggcaa cggccgttcc tgcgtgaaga tctcgttcgt
     2101 gacgccctgt tcgacgttgt aggcctcacc agcgaagagc tcgagcgact tgttctgggc
     2161 tttccaaccg aagcggctga tcgtaccgtc gttgccgttg tggttgaagg tgccggtgat
     2221 tccctggttg ttgttgaacg ccgcctgctg gttgttcagg agagtctcct cggagatggt
     2281 ctcgatcaga ccggtgccga aggttggcgt cgggatgcgg aagatgacgt tattgttgga
     2341 caactgaagc gcgaagccgg gctgcccgat actgcagggg cctgcgtcgc tgcggccggt
     2401 aatcgtgtac agatcttcca cacccccgtt gggctggctc gtgacaggat tgccaaacga
     2461 atctgtgaag aagatgaacc tcgcctcgcg caccggcccg gtcgaggtga tgaacgacgg
     2521 cacgctgttt tgtgcgccgt cagcggttgc atcagcgacc tgaggattcg tcgccggagc
     2581 agttccgccc actgccggct gcgcgtggca cgcggcgcag ctcctcgagt tgaaacgcgg
     2641 tcccaggccg acgccgttct cgcccgcgat ggttccagca accgagtcga tctcctggaa
     2701 tcgggccttg gcatcgttga agaagttgag aatggtgacg ggattattgg cggcgacgga
     2761 agcgagtggc gatccggcgg cagcaccgcc gcctcggacc cccggatctg tttgggcaaa
     2821 cccctgcaag acgaacagca acgaaaggaa tacagcgagc ggaacaactc gtcggatggc
     2881 catggtacct cctctaaagg gttaatagag acaggacacg agggcgaggc gagaggccga
     2941 cgcggcctcc gccctgatta ctacccgagg gagcggcagg agcggggaac cgagggagcg
     3001 gggaaccgat ggaaccggga acgtgggaac cggggaaacc ggggacgtta accccttccg
     3061 cttgtcatga aactgatacc gatcggttag gccttagtga cggcgaattg tggcgagcat
     3121 acatccatac gatagtcaat gacaactatg cgagtcggtt gcgtggcggg aaggatgcct
     3181 ttcggcatcc gcggacgaat ggggaggacc gcctaacccg gccccgctcg cgggctgagg
     3241 acccgcgaac tggttgcaat aacggcgctt ccgtcaggga cgttgggcgt cttgccctcg
     3301 tgaaaaacgg ctgctccgcg ccagcatcgc ggacacgtca ccccgctcgc ttcataggcg
     3361 tacgccgtgc gtctttttac aaggccgcgc gcagcgccgc cagcttctgc acgaaccttg
     3421 cgggctcgta gcgctcgcgg ttctggatct cggcgaagag ggcgtcgatc gcctcggtca
     3481 tcgggctctt gagcagtttc gggttgctgc gcgtcaacgc ggaggcgagg ctttgcaacg
     3541 ccagggcggt ctgctcggca gagtggacgt cggatgtgag gatgaaatcc gtgtcgccgg
     3601 cgatcgtgcg catcagcgcg cgcacgtcgt cgtcgcgcca gctcagcgcg gcgatctgcg
     3661 gggtgacgcc atcgatgacg cggcgggctt cgacggcggt ctggagcacg ccgttggaat
     3721 cgttcatgtg actcacgcgc gtggcgatga tggccacgtc gttgtcgagc tgtgcgcgcg
     3781 ccgagggatt ggcgcggccg acgaggagac ggaggacggc ccaatgctgc gggctccacg
     3841 cgggaagtcc ggcgcggccg ggccagccgc gctcctggcg ccatccgctc ccttccagcg
     3901 agtgatggca gttgatgcag ctcaggtcgg aaaactcggg ccacttctcc cctcgggcgt
     3961 ggcgggcgag attgtcgagt gactggctga acgcgacggc ctggccgacg gcgaaggcgc
     4021 gcgcgc
//
LOCUS       DATLVW010000234         5371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_177641, whole genome shotgun sequence.
ACCESSION   DATLVW010000234 DATLVW010000000
VERSION     DATLVW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177641"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VNN25_07485"
     CDS             <1..612
                     /locus_tag="VNN25_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91404.1"
                     /translation="TEPRWSSTMYGITFVVGCALSALAFVTFFLTRLVNTEAMGSLLK
                     PTHLRDLGNLMLALVMFYAYTTFSEFLLIWYANLHEEIPHFLVRTSGLWGAVAFSVAM
                     FHFFLPFGLLLIRPIKDRPKTIAIVAVIIIVMRYVALYWLVAPSWYGEHFTWSWMNLT
                     TLLGIGGVWIWAFIGQLRGQTIIPIHETWVEEAIREGALKVNG"
     gene            605..1060
                     /locus_tag="VNN25_07490"
     CDS             605..1060
                     /locus_tag="VNN25_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91405.1"
                     /translation="MADHAHYQDDDIVNPETRHEESDVNTRALLWSVVIFIVFAGLTY
                     AVLYLQFHAYARYFRHEASQPLTMMPRPSDASVPATPRLQPFPTKNQEGLMPPYAVTP
                     VTDMADMRAAQEKQLSEPGWVDQKKGIVRIPIETAKQLVVQRGLPVVTP"
     gene            1147..1929
                     /locus_tag="VNN25_07495"
     CDS             1147..1929
                     /locus_tag="VNN25_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXH91406.1"
                     /translation="MIFLFLAVALPAIAQTNSATPPQLPGKVAIDQKLNAQLPMDLML
                     RDESGRVVRLGQYFNHGRPVLLNFMYYRCPMLCPMVMDGIANGLTELRFDIGREFDVI
                     TVSVDPRDTPEQATAKKETYVKRYGRFGAANGWHFLTGPESAIKKLTNTVGFQYAYDI
                     KMDQFAHGAVLLALTPQGKVSRYLYGFEYKARDLRLALVEASAGKIGNASDAILLLCY
                     HYDPATGKYSRSAMNFVRAGGVATILSLAGFIFIMIRREHGR"
     gene            1926..2969
                     /gene="coxB"
                     /locus_tag="VNN25_07500"
     CDS             1926..2969
                     /gene="coxB"
                     /locus_tag="VNN25_07500"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXH91407.1"
                     /translation="MMLSDFPLWPEQASTVAPGVDALMTFMCIVCGGVSVITFFVIFI
                     LAIKYRRTPENQLAQDYEPPKALEAAWIIIPFLIFMFMFVWGSWLYFRIQRVPDNAID
                     IYATGKQWMWKFQHASGQREINTLHVPVGRPIRITMASEDVIHSLFFPVFRVKADVLP
                     NRYKTMWFQATKTGRFHIFCAEYCGTLHSGMIGWVEVMEPVDYQKWLAGGAEGSLASQ
                     GEKLFQKYACNTCHTNDATGRGPVLLGLYGTTVTLSNNATVTADENYIRESILNPQAK
                     IVKGFAPVMPTFQGQVSEDDLLKLVSYVHSLGTQKTAVVPVETPSSKATSAGSPLKPG
                     AGIPPQKDKGATP"
     gene            2966..4621
                     /locus_tag="VNN25_07505"
     CDS             2966..4621
                     /locus_tag="VNN25_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXH91408.1"
                     /translation="MSSLTLHDVRFVEPETTRNYITHDYGALSWLLTKDHKRIAILYL
                     ISITALFILGGFFAVVMRIELLTPEGDLLTSDTYNKFFTLHGIVMIFFFLIPSIPAVL
                     GNFLLPLMLGAKDLAFPKINLLSWYLYIIAAVMGLYIIITGGVDTGWTFYTPFSTQYS
                     NTHVFGVTFAAFIVGFSSILTGLNFLVTIHRMRAPGLTWFRLPLFVWAMYATSIVQLL
                     ATPVLAITLVMLMFERVVHVGIFDPALGGDPLLFQHMFWFYSHPAVYIMILPAMGVAS
                     EVVAAFTRKKIFGYTFVAYSSLAIAFLGFLVWAHHMFVAGISVYSAMIFSFLSYFVAV
                     PSAIKVFNWTATLYRASVALMTPMLYILGFIGLFTIGGVTGLFLAAIGLDVHVTDTYF
                     VVAHFHYVMVGGAVMAYMAGLHYWWPKITGRMYPEWWARLAALLVFAGFNLTFFPQFV
                     LGYLGMPRRYHAYAPEFQVLNVMSTAGASVLAVGYALPLCYLLWSLWYGRPAGPNPWG
                     AVGLEWATTSPPPMENFHETPVVTWDAYDYPAMMKAMGETQNV"
     gene            4614..5327
                     /locus_tag="VNN25_07510"
     CDS             4614..5327
                     /locus_tag="VNN25_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HXH91409.1"
                     /translation="MSDITLGGPPPAPRVEEHHEHHHPFLQHHFEDLGQQHEASTLGM
                     WVFLSTEILFFGGVLAAYWIYRVAYPEAWAIGAAQQIWWLGAINTIVLIISSLTMALA
                     VRNAQLGRRMGTVYMLIATLFFGSIFLGVKMFEYTKHWQEGLFPGAAFHWNEVPALAP
                     KVQLFMTFYWCLTGLHALHMIIGAGLLIWFIWRAWKGHFGPEYYGPVEIMGLYWHFVD
                     IVWIFLFPFLYLIHGAHHG"
BASE COUNT          956 a         1816 c         1471 g         1128 t
ORIGIN      
        1 acggagccga ggtggagctc gacgatgtac ggcatcacct tcgtcgtcgg ttgcgcgctc
       61 tcggctctgg ccttcgtcac gttcttcctc acgcgcctgg tgaacaccga agcgatgggc
      121 tccctgctca agccgaccca cctgcgcgac ctcggcaatc tgatgctggc gctcgtcatg
      181 ttctacgcct acacaacctt ctccgagttc ctcctcatct ggtacgcgaa cctgcacgag
      241 gagatcccgc acttcctcgt ccgcaccagc ggactctggg gtgctgtcgc gttctccgtg
      301 gccatgttcc acttcttcct gccgttcgga ttgctgttga tccgtccgat caaggaccgg
      361 ccgaagacga tcgcaatcgt tgccgtgatc atcatcgtga tgcgttacgt ggccctctac
      421 tggctcgtcg ccccatcgtg gtacggcgaa catttcacct ggtcgtggat gaacctcacc
      481 acgctgctcg gcatcggcgg cgtctggatc tgggccttca tcggccagct ccgcggacag
      541 accatcattc cgattcacga gacgtgggtc gaagaagcca ttcgggaagg agcgctgaag
      601 gtcaatggct gatcacgctc actaccagga cgacgacatc gtcaatcccg agacgcgcca
      661 cgaagagagc gacgtcaaca cccgtgcgct tctttggtct gtcgtgatct tcatcgtctt
      721 tgcgggcctc acgtacgccg tgctctatct ccagtttcac gcctacgccc gctatttccg
      781 gcacgaagcc tcgcagccgc tgacgatgat gccgcgcccc tcggacgcct cagtgccggc
      841 cacgccacgc ctgcagcctt tcccgacgaa gaatcaagaa gggttgatgc cgccctacgc
      901 ggtgacgccg gtgaccgaca tggccgatat gcgcgctgca caggagaagc agttgagcga
      961 gcccggctgg gtcgatcaga agaagggcat cgtgcggata ccgatcgaga ccgcgaagca
     1021 gctcgtcgtg cagcgtggac tgccggtggt gacgccatga cgttttatcc gcagatgacg
     1081 cagattcaca cagataaaag caatgcagct catctgcgtt catttgcgaa atctgcggac
     1141 aagcttttga tcttcctgtt cctggcagtc gcgctcccgg caattgcaca gaccaactcc
     1201 gccacgcccc cccagctccc cggcaaggtc gccatcgacc agaaactcaa cgcgcagctt
     1261 ccgatggatc tgatgctccg cgatgaatcc ggccgcgtcg tccggctcgg ccagtacttc
     1321 aatcatggcc gtccggtgct gctcaacttc atgtactacc gctgccccat gctctgcccg
     1381 atggtcatgg acggcatcgc caacggcctg accgagctgc gattcgacat cgggagagag
     1441 ttcgacgtca tcaccgtcag cgtcgatccg cgcgacaccc ccgagcaggc cacggccaag
     1501 aaagaaacct atgtcaaacg ttacggccgt ttcggcgccg ccaacggctg gcacttcctg
     1561 accggtcccg aaagcgcgat caagaagctc accaacacgg ttggattcca gtatgcgtac
     1621 gacatcaaga tggaccagtt cgcgcacggc gcggtgctgc tcgcgttaac accgcagggc
     1681 aaggtatcgc gctacctcta cggcttcgag tacaaggcgc gcgatctccg tctcgccctc
     1741 gtcgaagcct cggccggcaa gatcggcaac gccagcgacg ccatccttct cctctgctac
     1801 cactacgatc cggccaccgg caagtacagc cgctcggcca tgaacttcgt ccgcgctggc
     1861 ggcgtcgcca cgattctcag cctcgctggc ttcatcttca tcatgattcg tagagagcac
     1921 ggacgatgat gctttccgat ttcccgctgt ggcccgagca ggcctcgacc gtcgctcccg
     1981 gcgtcgatgc gttgatgacg ttcatgtgca tcgtctgcgg tggcgtgtcg gtgatcacgt
     2041 tcttcgtgat cttcattctg gccatcaaat accgccgcac gcccgagaac cagttggcgc
     2101 aggactacga gccgccgaaa gcgctcgagg cagcctggat catcattccc ttcctcatct
     2161 tcatgttcat gttcgtgtgg ggatcgtggc tctacttccg gattcagcgc gtccccgata
     2221 acgcaatcga catctatgcc accggcaaac agtggatgtg gaagtttcag cacgccagcg
     2281 gacagcgcga gatcaacacg ctgcacgtcc cggtaggacg tccgatccgc atcaccatgg
     2341 cctccgagga tgtgattcat agcttgttct ttccggtgtt ccgcgtgaag gcggacgtcc
     2401 tgccgaaccg ctacaagacg atgtggttcc aggccacgaa gaccggacga ttccacatct
     2461 tctgcgccga gtactgcggc acgctccact ccggcatgat cggctgggtg gaagtgatgg
     2521 agccggttga ttatcagaaa tggctcgctg gtggcgccga gggctcgctg gcatcgcagg
     2581 gcgaaaagct gttccagaaa tacgcgtgca acacctgcca cacgaacgac gccacgggcc
     2641 gcgggcctgt cctcctcggt ctgtacggca cgaccgtcac gctgtcgaac aacgccaccg
     2701 tcacggctga cgagaactac atccgcgagt cgatcctcaa cccgcaggcg aagatcgtca
     2761 aaggcttcgc gccggtcatg ccgacgttcc agggacaggt cagcgaagac gatctgctca
     2821 agctcgtctc gtatgtccat tccctcggta cgcagaagac ggcggtcgtt cctgtcgaga
     2881 ctccgtcatc gaaggccacc agcgccggct cgccgctcaa accgggcgca ggcattcccc
     2941 cgcaaaaaga caaaggtgcc acgccatgag ctcattgaca ctgcacgacg tccgcttcgt
     3001 cgagccggaa acgacgcgca actacatcac gcacgactac ggcgcgctct cctggctcct
     3061 gacgaaggac cataagcgga tcgccatcct ctatctcatc tccatcaccg cgctcttcat
     3121 cctcggcgga ttcttcgccg tggtcatgcg catcgagctg ctgacgccgg aaggcgatct
     3181 gctcaccagc gacacctaca acaaattctt cacgctgcac ggcatcgtga tgatcttctt
     3241 cttcctgatt ccgtcgatcc cggcggtgct cggcaacttc ctgctgccgc tcatgctcgg
     3301 tgcgaaagat ctggccttcc cgaagatcaa cctgctgagc tggtacctct acatcatcgc
     3361 ggccgtgatg gggctgtaca tcatcatcac aggcggcgtc gacaccggct ggacgttcta
     3421 cacgcccttt tcgacgcagt actcgaacac gcacgttttc ggcgtgacgt tcgcggcctt
     3481 catcgtcggt ttctcgtcga ttctcaccgg cctcaacttc ctcgtcacga tccatcgcat
     3541 gcgcgcgccg ggactgacct ggttccgcct tccgctgttc gtgtgggcga tgtacgccac
     3601 gagcatcgtg cagctcctgg ccacgcccgt tctcgccatc acgctggtca tgctgatgtt
     3661 cgagcgcgtc gtccacgtcg gcatcttcga tcccgcgctc ggcggcgatc cgctcctctt
     3721 ccagcacatg ttctggttct actcccaccc ggccgtctac atcatgattc tgccggcgat
     3781 gggcgtggcg tcggaggttg tggccgcgtt cacgcgcaag aagatcttcg gctacacgtt
     3841 cgtcgcgtac tcctctctcg cgatcgcctt cctcgggttc ctggtgtggg cgcaccacat
     3901 gttcgtcgcc ggcatctcgg tctattcggc gatgatcttc tccttcctca gttacttcgt
     3961 cgccgtcccc tcggctatca aggtcttcaa ctggacggcc actttgtatc gagcgtccgt
     4021 cgcgctcatg acgccgatgc tctacatcct cggtttcatc ggactcttca ccatcggcgg
     4081 tgtgacggga ctcttcctcg ccgcaatcgg actcgacgtc cacgtcaccg acacctactt
     4141 cgtcgtcgct catttccact acgtcatggt cggcggcgcg gtcatggcct acatggccgg
     4201 actgcactac tggtggccga agatcaccgg ccggatgtat cccgagtggt gggcgcgcct
     4261 cgctgcgttg ctcgtcttcg ccggcttcaa cctgaccttc ttcccgcagt tcgttctcgg
     4321 ctacctcggc atgccgcgcc gctaccacgc ctacgcgccg gagttccagg tgctcaacgt
     4381 catgtccacc gccggcgcct cggtgctggc tgtcggctat gccctgccgc tctgctatct
     4441 gctctggtca ctctggtacg gccgccccgc cggaccgaat ccgtggggcg cagtgggatt
     4501 ggagtgggca acaacgtcgc cgcctccgat ggaaaacttc cacgagacgc cggtcgtgac
     4561 atgggatgcg tacgactacc cggccatgat gaaggcgatg ggggagacgc agaatgtctg
     4621 acatcaccct cggcggtccc ccgcccgcgc cgcgcgtcga agaacatcac gaacatcacc
     4681 acccgttcct ccagcatcat ttcgaggacc tcgggcagca gcacgaagca tccacgctcg
     4741 gaatgtgggt gttcctctcg acggagatcc tcttcttcgg tggcgtgctt gccgcgtatt
     4801 ggatctaccg cgtcgcgtat cccgaggcgt gggcgatcgg tgccgcgcaa cagatctggt
     4861 ggcttggcgc catcaacacc atcgtcctga tcatcagctc tctgacgatg gcgctcgccg
     4921 tccgcaacgc gcaactcggg cggcgcatgg gcaccgtcta catgctcatc gccacgctct
     4981 tcttcggcag catcttcctc ggcgtgaaga tgttcgagta cacgaaacac tggcaggaag
     5041 ggctcttccc gggagccgcc ttccactgga acgaagtccc cgcgctcgct ccgaaggtcc
     5101 agctcttcat gaccttctac tggtgcctga cggggcttca cgcgctgcac atgatcatcg
     5161 gtgccggcct tctgatctgg ttcatctggc gcgcctggaa aggtcacttc ggccccgaat
     5221 actacggacc ggtcgagatc atgggtctct actggcactt cgtcgatatc gtctggatct
     5281 tcctcttccc gttcctctac ctcattcacg gagcgcatca tggctgacgt gaccacgacc
     5341 gaaatcaacc attccgagca cgcaccgcac g
//
LOCUS       DATLVW010000235         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_298140, whole genome shotgun sequence.
ACCESSION   DATLVW010000235 DATLVW010000000
VERSION     DATLVW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298140"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..551)
                     /locus_tag="VNN25_07515"
     CDS             complement(102..551)
                     /locus_tag="VNN25_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXH91410.1"
                     /translation="MAFGAGGGSPDEVKGDINVTPLVDVCLVLLIIFMVVTPMLQSGV
                     DVQLPQGPHSEKKPGKESDLIVSIKQDGTVFIGQDWIPDRDLPRYMKAEHDKEAGRNV
                     MIKADRRINFGKVRMVMKAANDADFTAVSFLTENQMAGGKIAQEHGM"
     gene            complement(561..1274)
                     /locus_tag="VNN25_07520"
     CDS             complement(561..1274)
                     /locus_tag="VNN25_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXH91411.1"
                     /translation="MDNNSFSLLGMLATMNWVARGVVVVLALMSIWSLTIAIERIVRF
                     QRAKRESLGIALGVTPLLKQHKLEEAITMTADKKYRHSHLARVLRAGLTEFQYESTQS
                     LPDDFDIVASGQRAIERETLMTTAEMKKGLGNLATISTTAPFVGLFGTVAGIIHAFQG
                     MAISGSGGLGAVSAGIAEALVTTAFGLFVAIPAVWLYNYFLNKVERFQVEMSNSSSQL
                     IDYFIKNEARVRANAAAGR"
     gene            complement(1361..>2180)
                     /locus_tag="VNN25_07525"
     CDS             complement(1361..>2180)
                     /locus_tag="VNN25_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH91412.1"
                     /translation="SALGIIPEGAQQYPQEVADMFDDVLIESAGKDKKKGGWKTVLIS
                     GFLHLVVIGAVVAAGYYVKKNPEIITKPIQAFVVSAPPPPPPPPPAASASSTPHVAHV
                     ETPKTQPTFHQPTHIPREVPQTEAPTDTAGAQEGGVVGGQTGGVVGGVVGGVVGGTLG
                     GTLGGQLGGQLGGQGDKPMRVGGDVNPPVATNRIEPVYTEVARKAKVEGLVIVETIID
                     RNGNVTDVRVLKGLPMGLDQSAIDAVKRWKFKPGTLNGQPVPVIFTLTVNFKMQ"
BASE COUNT          364 a          683 c          677 g          456 t
ORIGIN      
        1 tgacattggt tttctcctgg gggtcgcgtt tcgggagtcg ggtgtcggag attcgattct
       61 tctctacgac tcccgacccc cgacgcgcga cccccgcata ctcacattcc gtgttcctgc
      121 gcgatcttcc cgccggccat ctggttttcg gtcaggaacg agacggccgt gaagtcggcg
      181 tcgttggccg ctttcatcac catccgcact ttgccgaaat tgatgcgccg gtcggctttg
      241 atcatgacgt tgcggcccgc ttctttgtcg tgctcggcct tcatgtagcg gggtaaatcg
      301 cggtcaggaa tccagtcctg accgatgaag accgttccgt cctgtttgat cgagacgatc
      361 agatcggact cttttcccgg cttcttctcc gagtgcggcc cctgcgggag ttggacgtcg
      421 acgccggact gcagcatcgg cgtcaccacc atgaagatga tgagcagcac gagacagacg
      481 tccacgagcg gcgtgacgtt gatgtctcct ttgacttcgt cggggcttcc gcccccagcg
      541 ccgaatgcca tcggtgactc ctaacggccc gcagccgcat tggcgcgaac gcgtgcttcg
      601 ttcttgatga agtagtcgat gagctgcgac gacgagttcg acatctccac ctggaagcgc
      661 tcgaccttgt tgaggaagta gttgtagagc cacaccgcgg ggatggccac gaagaggccg
      721 aacgcggtgg tcacgagtgc ttcggcgata ccggccgata ccgcgccgag gccgccggag
      781 ccgctaatgg ccatgccctg gaaggcgtgg atgatgccgg cgacggtgcc gaagaggccg
      841 acgaacggag cggtcgtcga gatcgtggcc agattgccga gacccttctt catctccgcg
      901 gtggtcatca gcgtctcgcg ctcgatggcc cgttgtcccg aggcgacgat gtcgaaatcg
      961 tcgggcagcg actgcgtcga ctcgtactgg aactcagtca gccccgcgcg caacacgcgt
     1021 gcgagatgcg agtgccggta cttcttgtcg gcagtcattg tgatggcctc ttcgagcttg
     1081 tgctgtttga gcagcggcgt aacgccgagg gcgatgccga gcgactcgcg cttggcgcgc
     1141 tggaaacgga cgatccgctc gatggcgatc gtgagcgacc agatcgacat gagcgccagc
     1201 acgacgacca cgccgcgggc cacccagttc atcgtcgcca acatcccgag gaggctgaaa
     1261 ctgttgttat ccatgcactt ctcctttttc cagtggccag cgtatcaggg cggcggccag
     1321 ggtttgtact ctgacgcgtg ccgtagcctg gtcacttatc tcattgcatc ttgaaattca
     1381 ccgtgagcgt aaagatgaca ggtaccggct gcccgttcag cgtgccgggc ttgaacttcc
     1441 agcgcttgac tgcatcgatc gccgactggt caaggcccat cggcagacct ttgagcacgc
     1501 gaacatctgt cacgttgccg ttccggtcga tgatcgtctc cacgatgacc aatccttcca
     1561 ctttggcttt gcgcgccacc tcggtgtaga ccggctcgat ccggttcgtg gcgaccggcg
     1621 gattgacgtc gccgccgacg cgcatcggct tgtcgccctg gcccccgagt tgtccgccga
     1681 gctggccgcc cagggtgccg ccgagcgtcc ctccgacgac tccgcccacc acaccgccca
     1741 cgacgccgcc agtctggccg cccactacgc caccttcctg ggccccggcc gtgtcggtcg
     1801 gagcttcggt ctgcggcact tcgcgcggta tgtgcgtcgg ctgatgaaac gtcggttgcg
     1861 tctttggagt ctcgacatga gccacatgcg gcgtggacga agccgatgcc gcgggcggcg
     1921 ggggaggtgg aggcggcggt gccgagacga cgaaggcctg aatcggcttg gtgatgatct
     1981 cggggttctt cttgacgtag tagccggcgg cgacgaccgc tccgatgacc accaggtgca
     2041 ggaagccgga gatgagaacc gtcttccacc cacccttttt cttatccttg cctgcggatt
     2101 cgatcaggac gtcatcaaac atatcggcta cctcttgcgg atactgctgt gctccttcag
     2161 ggatgatgcc aagtgcggag
//
LOCUS       DATLVW010000236         7606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_257430, whole genome shotgun sequence.
ACCESSION   DATLVW010000236 DATLVW010000000
VERSION     DATLVW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257430"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VNN25_07530"
     CDS             complement(<1..833)
                     /locus_tag="VNN25_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91413.1"
                     /translation="MDFIKDFVNVLSAGSISFLLLTSIFALIIYFNLLNGRIKSGGGI
                     AFLGFGIILLVVGIFIRPLFYLGVAYLLILFGLAALGGRLWDTKVGLRLFVVGGLAFG
                     LSMLDPNFFLIAAKPDNVPIVAMIFLLGFFTWLALRKAYLNDSQLALGGIPWEKTESD
                     EKLFTWPDLVYTEMLCMILLTIVMIVWSVVLRAPLEEAANPTSSPNPAKAPWYFLGLQ
                     EMLVYFDPWLAGVVLPTLIIVGLMAIPYIDTNPKGNGYYSFRERKWEIGVFLYGFLVL
                     WS"
     gene            complement(976..1761)
                     /locus_tag="VNN25_07535"
     CDS             complement(976..1761)
                     /locus_tag="VNN25_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXH91414.1"
                     /translation="MANLTALKDGIVNSQVWKSVFRVGVPNTNRKRVLAVLGSLILHL
                     HPVKVRRSGVRMSYTWCMGGTSFFLFLVETVTGLLLMFYYRPTIEYAFTDIMDLREQA
                     PFGIMREMHRWGAHAMVIAVWLHMFRVFMTGSYKPPREFNWNVGVILLVLTLLLSFTG
                     YLLPWDQLAIWAITVGSSMARASPGAGNEGPMSGLVKLGDIRLIHAGDDARFALIGGR
                     SIGAATLLRFYVLHCVVIPLAAGFLMAIHFWRVRKDGGISGPL"
     gene            complement(1770..2342)
                     /locus_tag="VNN25_07540"
     CDS             complement(1770..2342)
                     /locus_tag="VNN25_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXH91415.1"
                     /translation="MSEEIKPYQLVPKTPLAGSAAADIREQGEISRRSFPFVLTTAWL
                     ALAAALGGLASILGRFLFPNVLFEPVQKFKAGLVADYSVGEVDERWKDKYGIWIVRTE
                     EIVYALITVCTHLGCTPNWLPTQNKFKCPCHGSGYYKSGINFEGPTPRPLERAKIFID
                     VADGQIVVDKSKQFHQERGEWTDPEAFIKL"
     gene            complement(2905..3816)
                     /locus_tag="VNN25_07545"
     CDS             complement(2905..3816)
                     /locus_tag="VNN25_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH91416.1"
                     /translation="MHENSAANESPTPYSPLPTPTPTDVPKESSRTILFQFVVFPLGI
                     VFIAVLIFFLFGKLATDEQTIPDYLNSIRSGSSHERWQAAYQLSKSLKRGEASRYPNL
                     EPQVALLYTNSKTDDPRIRRYLGMVLGTLGDRRATPLLLEGLDDKDTDNRVYALMALG
                     ELHDPASVPRIAKAAADEDKDIRKTAYFTLGEIGDGSAVPVLVNGLQDEVADVRWNAA
                     VSLARLGDRRAIGPLREMLDRSRLNAVAGMREDQKEDAMIVAMSAYVKVAGRDAMPDL
                     QRIAASDPSLRVQAAAKQALQAVEKKM"
     gene            complement(3912..4385)
                     /locus_tag="VNN25_07550"
     CDS             complement(3912..4385)
                     /locus_tag="VNN25_07550"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXH91417.1"
                     /translation="MPHFITDKCIGCSVCEVKCPTNTIWGAARGMYYIHPERCIDCSV
                     CGIYCPVDAIQNQHGEFIPRVKPKEIPKAEVIKELCTGCEFCVDICPFECIKMIDPND
                     ETVANHYKIAFVDEKKCVACRLCESVCDKDAIVVPEAPTQLKTYLPDFVVMGTGR"
     gene            complement(4397..5716)
                     /locus_tag="VNN25_07555"
     CDS             complement(4397..5716)
                     /locus_tag="VNN25_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91418.1"
                     /translation="MSVDRGSRESGVGSQSLTAGHFRFALVLLLLLLFLALRVPLLFY
                     REPFFDELFTRWIAGKSFAGILAALRLDSGPPLYYFVVHAMGDPSVVATRVLSLICAA
                     VSVVVILRATRWAPAAAMLIAVFPPAVLFAVDARAYALCAMFVTIAVLAIDRDWPFVA
                     SVALVLAAYSHYYGVLFFPILLVTAAGPAAIQPAGRRRYTWFRRSASLALAVVLFVPG
                     FWLAMRQPAGAREWMTSSWPDGLFVRPPLALTIVGAIAVLLSLRINRYLFMVIVPLVL
                     AIALRVYVPMRFEAVVAAPLVLWLAESLRQNRFRLVLMAALVGVGVTWSALGILDHAG
                     RPADPYRQTAQWVAGNIAANQNVVASGYCYLETLMNGHQRLTAFPPEQAEHPGWRALP
                     QAGLRSPAGLFFWIGERNAPELDVFVRERRRIQPLFINDRAMVALVR"
     gene            complement(5713..6096)
                     /locus_tag="VNN25_07560"
     CDS             complement(5713..6096)
                     /locus_tag="VNN25_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91419.1"
                     /translation="MALENANFLVVEDSPTMRQLISFSLKRFKNARIIEAVDGVDALK
                     KLSGPDKIDLILTDINMPVMDGLKLVSLVRQNAQLRNIPIIIITTEGAEEDRERGLAL
                     GANAYISKPIQSSHLIKTISELLAV"
     gene            complement(6110..6916)
                     /locus_tag="VNN25_07565"
     CDS             complement(6110..6916)
                     /locus_tag="VNN25_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXH91420.1"
                     /translation="MTEERAEKFLELFNKGKEFTEELLRENQRLRYRLATLETGSTAG
                     VDEEVQRLRAEVQHLTEENRRIARRFKEVEEENKDFANRYIEVEEQNNNLANLYVASY
                     QLHSTLDFREVIQIVQEIVINLIGAESFAILLLDEKTNELKTIASEGEDVMPGIETIS
                     TRLGDGIMGGVARTGESYYINQDMEGGKVTLEKPLAAVPLKIKEHVIGLVVIYKLLQQ
                     KDSFSAVDYELFSLLAAHAATAIFSSKLYSQSERKLNTIQSFLDLLTTTS"
     gene            7049..7489
                     /locus_tag="VNN25_07570"
     CDS             7049..7489
                     /locus_tag="VNN25_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HXH91421.1"
                     /translation="MTMEADPVVDRLEDQISWYDRKSSSNQKTYKRIKFTEIVAAAVI
                     PFLPALPLVHADWIAGGLGVLITILEGALHLNQYQENWIAYRSTCESLKHEKYVYIAK
                     ASPYAGVADPRALLAERVESLVSQEHAKWASSQQENTKAKPATT"
BASE COUNT         1625 a         2215 c         2348 g         1418 t
ORIGIN      
        1 gaccagagca cgaggaagcc gtagagaaag acgccgatct cccactttct ttcacgaaac
       61 gagtagtagc cgttgccctt cggattggta tcgatgtacg ggatggccat cagtccgacg
      121 atgatgagcg tcggcaggac gacgccggcc agccaggggt cgaagtaaac gagcatttcc
      181 tgcaggccga ggaagtacca gggagccttg gcaggattcg gtgatgacgt cggattggct
      241 gcttcttcga gtggtgcgcg cagcacgacc gaccagacga tcatcacgat cgtcagcagg
      301 atcatgcaga gcatctcggt gtagacgagg tcgggccagg tgaagagctt ttcgtccgac
      361 tcggtcttct cccacggaat gccgccgagc gcgagctgcg aatcgttcag atacgccttg
      421 cgcagtgcca gccaggtaaa aaatccgagg agaaagatca tcgccacgat ggggacgttg
      481 tcgggcttgg cggcgatcag gaagaagttc ggatcgagca tcgacaggcc gaatgccagc
      541 ccaccgacga cgaagagccg aaggccgacc ttcgtatccc acagccgccc gcctagtgcc
      601 gcaagcccga agagaatcag caggtaggcg acgccgaggt aaaacagcgg gcggatgaaa
      661 atgccgacca cgaggagaat gatgccgaag ccgagaaagg cgattccgcc gcccgatttg
      721 atccggccgt tgagaagatt gaaatagatg atcaacgcga aaatcgacgt caacaacagg
      781 aacgagatcg atcccgcgga gagtacgttg acgaaatcct tgatgaagtc catcagcgcg
      841 tggcctccgt ggggagggtg tcgggagtcg ggggtcggaa gtcggagaaa ccggaggacg
      901 atgttcgcgc cggtgtgcgc tccggcaccc gacacccgac tcccgacacc cggctgactg
      961 accagcgaag agttctcata gcggacccga gattcctccg tcttttcgca ctcgccagaa
     1021 gtgaatggcc atcaggaagc cggccgccag cggaatgacg acgcagtgca gcacgtagaa
     1081 ccgcagcagc gtggccgcgc cgatcgatcg gccgccgatg agcgcaaatc gggcgtcatc
     1141 acctgcgtga atgagcctga tgtcgcctag tttgaccagc ccggacatgg gtccctcatt
     1201 accggcgccg ggagaggcgc gcgccatcga agatccaacc gtgatggccc agattgcgag
     1261 ctgatcccac gggaggagat atcccgtaaa cgagagcagc agcgtgagga cgaggagaat
     1321 gacgccgacg ttccagttga attcgcgcgg cggcttgtac gagccggtca tgaagacgcg
     1381 gaacatgtgc agccagacgg cgatgaccat ggcgtgcgcg ccccatcggt gcatctcgcg
     1441 catgatcccg aatggagcct gctcgcgcag atccatgatg tcggtgaagg cgtattcgat
     1501 ggtcggccgg tagtagaaca tcagcagcag gccggtgacc gtttcgacga ggaagaggaa
     1561 gaacgacgtc ccgcccatgc accaggtgta gctcatgcgc acgcccgagc ggcgcacttt
     1621 caccggatgc agatgcagga tgagcgagcc caggacggcc agaacacgct tacggttcgt
     1681 gttggggaca ccgacacgga agacggactt ccagacctgt gagttgacga tgccgtcttt
     1741 gagtgccgtc aggttcgcca ttcagctccc tataacttga tgaacgcttc cgggtcggtc
     1801 cactcgccgc gctcctggtg aaactgcttc gacttgtcga cgacaatctg gccgtcggca
     1861 acatcgatga agatcttggc gcgctcgagc gggcgtggag tcggcccttc gaagttgatc
     1921 cccgacttgt agtagccgct gccgtggcag gggcatttaa acttgttttg cgtaggcagc
     1981 cagttcggcg tgcagccgag atgggtgcag acggtgatca gggcgtacac gatctcctcg
     2041 gtgcgcacga tccagattcc gtatttgtcc ttccagcgct cgtccacctc gccgacggag
     2101 tagtcggcga ccagcccggc cttgaacttc tggaccggct cgaagaggac gttggggaag
     2161 agaaagcggc ccagaatgct ggccagaccg cccagtgctg ccgcgagagc cagccaggca
     2221 gtggtgagaa cgaaggggaa actacgcctc gagatctccc cctgttcgcg tatatcggca
     2281 gccgcactcc ctgcgagggg agtcttcgga accaactgat atggcttgat ttcttcagac
     2341 atccgaggtc gtgatggggc cgaatcgagg ccgttccggc gcgggcgtaa atatctgctt
     2401 ttcgtcaatc gctgtcaacg aaaaaggtga cgccggacac aggttgacgt gatgtggttt
     2461 cggcagtttc cagtgttcgg ttggtgtgtg tacttcccgg cgatgagtgt ccagggtccg
     2521 gcggatcgct cctgcgagct acttggatag ttgtctcgag gaaactgtgc gtactgggaa
     2581 aaatttgccg aatcttgaag acaatttcct caaagttgag gacgtttgtc ctgaaagttg
     2641 aggaccttcg tcatgaaagt tgaggacctt ggtcatgaaa attgatgacc ttcgtcatga
     2701 aacttgatga ccttcgtcat gaaagttgat gaccttcgtc atgaaagttg atgaccttgg
     2761 tcatgaaaat tgatgacctt ggtcatgaaa cttgatgacc ttggtcatga aagttgatga
     2821 ccttcgtcat gaaaattgat gaggtttgtc ctcaaagttg gacattcctc taaaaccagg
     2881 cagcggaagc agctccgcaa cgacctacat cttcttctcc accgcctgca acgcctgctt
     2941 tgccgctgct tgtacccgaa ggctgggatc gctcgcggcg atgcgctgaa gatcgggcat
     3001 cgcatcgcgg ccggccacct ttacgtaggc agacatggcc acgatcatgg cgtcttcctt
     3061 ttgatcctcg cgcatgcctg ccacggcatt gagccgcgag cggtcgagca tctcgcgcag
     3121 cggtccgatg gcgcgccggt cgccgaggcg ggccagcgag acggcggcgt tccagcgaac
     3181 gtcggcgact tcgtcctgca gaccattcac gaggaccgga acggcgctgc cgtcgccgat
     3241 ctcaccgagc gtgaagtagg cggtcttgcg gatgtccttg tcctcgtccg cagcagcttt
     3301 tgcgatgcga gggacggagg ctggatcgtg aagctcgccg agcgccatca gcgcgtagac
     3361 gcggttatcg gtgtctttgt cgtcgagacc ctcgaggagc agcggcgtgg cgcggcggtc
     3421 gccgagggtg ccgagcacca tgccgaggta gcggcggatg cggggatcgt cggttttcga
     3481 attggtgtag agcaacgcaa cctgcggctc gagattcgga tagcgcgacg cctcgccgcg
     3541 cttgagtgat ttcgagagct gatacgccgc ctgccagcgc tcgtgcgaac tgccggagcg
     3601 gatggagttg aggtaatcag gaatggtctg ctcgtcagtg gccagtttgc cgaagaggaa
     3661 gaagatcagg acggcaatga agacgatgcc gagcggaaag acgacgaact ggaagaggat
     3721 cgttcgggag gattctttgg ggacgtctgt gggggtgggt gtggggagtg gggagtaggg
     3781 ggtgggagat tcgtttgcgg cagagttttc gtgcattgaa ttaggttcgg aggtcgggag
     3841 tcgggagtcg gcgttcggcg tgggttgctt cttctccgac tccccacccc ccactcccca
     3901 ctcccccccg ctcaacgtcc cgtgcccatc acgacaaagt cagggaggta cgtcttgagc
     3961 tgcgtcggcg cctcggggac aacgatcgca tccttgtcgc agacggactc gcagagccgg
     4021 caggccacgc atttcttctc gtcgacgaag gcgatcttgt agtggttggc gaccgtttcg
     4081 tcgttcggat cgatcatctt gatgcactcg aacggacaga tgtcgacgca gaactcgcat
     4141 cccgtgcaaa gctctttgat gacttcggct ttcgggatct ctttcggctt cacgcgcgga
     4201 atgaactcgc cgtgctggtt ctggatggcg tcgaccgggc agtagatgcc gcagacggaa
     4261 cagtcaatac agcgctcagg atggatgtag tacatccctc gggccgcacc ccagatcgtg
     4321 ttcgtggggc acttgacctc gcagacgctg catccaatgc acttgtcggt gatgaaatga
     4381 ggcatgggct cggagtttac cttaccagtg caaccatcgc acggtcgttg atgaagagtg
     4441 gttggatcct gcggcgttcg cggacgaaga catcgagctc gggcgcgttg cgttcaccaa
     4501 tccagaagaa caaccccgca ggcgatcgca gtcccgcctg cggcagcgcc cgccagcccg
     4561 gatgctccgc ctgctccggc ggaaacgcgg tcagacgctg gtggccattc atcaacgtct
     4621 cgagatagca atatccggac gcgacgacat tctgattcgc ggcgatgttt cccgcgaccc
     4681 actgtgcagt ctgacgatac ggatcggccg gccgtccggc gtgatcgagg atgcccagcg
     4741 cgctccacgt gacgccgaca ccgaccagcg ccgccatcaa caccagacga aaccggttct
     4801 gtcgcagcga ttcggcaagc cagagcacca ggggcgccgc gaccactgcc tcgaatcgca
     4861 tcgggacata gacccgcaaa gcgatcgcaa ggaccagcgg aacgatgacc atgaatagat
     4921 aacgattgat ccgcagcgac agcagaaccg ctatcgcacc gacgatcgtc agcgcaagcg
     4981 gaggccgcac gaacaaccca tcaggccacg agctcgtcat ccactcccga gctcccgccg
     5041 gctgccgcat cgccaaccaa aaccccggca caaacagaac caccgcaagc gccaaggaag
     5101 cagagcgccg aaaccaggtg tagcgccggc ggcccgccgg ctgtatcgcc gccggcccgg
     5161 cggcggttac cagaagaatc ggaaagaaca acaccccgta gtaatgcgaa tacgcggcaa
     5221 gaaccagcgc gacagaagcg acaaatggcc aatcccgatc gatcgccagc accgcgatcg
     5281 tcacgaacat cgcgcacagc gcatacgcac gcgcatccac cgcgaagagc accgcaggag
     5341 gaaagaccgc aatcaacatc gcagcagccg gcgcccaacg tgtcgcccgc aagatcacaa
     5401 cgaccgaaac cgccgcgcag atcagcgaca acacacgcgt cgccacaacc gacggatcgc
     5461 ccatcgcatg cacgacgaag tagtagagcg gcggtccgga atcaagccgc agcgccgcca
     5521 gaatcccggc gaacgacttg cccgcaatcc atcgcgtgaa cagctcgtcg aaaaagggct
     5581 cgcgatagaa gagaagcgga acgcgcagcg cgaggaatag aagaagtagt agaagaacga
     5641 gcgcgaaacg gaagtggccg gctgtcaggc tctgactccc gacccccgac tcccggcttc
     5701 cgcgatcaac actcacaccg ccagcaactc cgagatcgtc ttgatcaggt gcgacgactg
     5761 aatcggcttg gagatgtagg cgttggcgcc gagggccaga ccgcgctcgc gatcttcttc
     5821 cgcaccctcg gtcgtaatga tgatgatggg gatgttgcgg agctgcgcgt tctggcggac
     5881 gagcgatacg agcttcagtc cgtccattac cggcatgttg atgtcggtga ggatgagatc
     5941 gatcttgtcg ggcccggaga gtttcttgag cgcgtcgacg ccatcgacag cctcgatgat
     6001 cctggcgttc ttgaatcgct tcagcgaaaa cgagatcaac tgccgcatgg ttggcgagtc
     6061 ttcgacaacc agaaagttcg cgttctcgag tgccaaagtt atccgcccct cacgatgtgg
     6121 tcgtcagcag gtcgaggaag ctctgaatcg tgttcagctt ccgctccgac tgcgaataga
     6181 gtttggacga gaagatcgct gtcgctgcgt gcgcggcgag cagcgagaag agctcgtagt
     6241 cgaccgccga gaacgagtct ttctgctgga gcaacttgta gatcacgacc agcccgatga
     6301 cgtgttcctt gatcttcagc ggaacagcag ccagcggctt ctccagcgtt accttgccgc
     6361 cttccatgtc ctgattgatg tagtagctct cgccggtcct ggccacgccg cccatgatgc
     6421 cgtccccgag ccgcgtcgag atggtctcga tgccgggcat gacatcttcc ccttccgagg
     6481 cgatggtctt gagctcgttc gtcttctcgt cgagcagcag gatcgcgaac gactccgcgc
     6541 cgatcagatt gatgacgatc tcctgcacga tctggatgac ttcgcggaag tcgagcgtgg
     6601 agtgaagctg gtagctggcc acgtacaggt tagcgaggtt gttgttctgc tcttcgacct
     6661 cgatgtagcg gttggcgaag tccttgttct cttcctcgac ttccttgaaa cgccgcgcga
     6721 tgcgccggtt ttcttcagtg aggtgctgca cttccgcgcg cagccgctga acctcttcgt
     6781 cgacgccggc ggtcgatccg gtttcgagcg tggcgaggcg ataccgaagg cgctggtttt
     6841 cccgcagcaa ctcctcggtg aattccttcc ccttgttgaa aagctcgagg aacttctccg
     6901 ctctttcttc cgtcatgaaa atcccgcttg gaccgacgcg gagagtagca cagggagcgt
     6961 ggccgttggg acagcgaaag gaaagtgatc gccgtcgggc accgcaacgg gcgctacaat
     7021 agatccatca atcaatcttg gagtggccat gacgatggaa gcagatccgg tggtggatcg
     7081 tctcgaggat cagatctcgt ggtacgaccg caagagcagc agcaaccaga aaacgtacaa
     7141 gcgcatcaag ttcactgaga tcgttgcggc cgcggtcatc ccgttcctcc cggcgcttcc
     7201 gctggtgcac gctgactgga ttgccggcgg gctcggcgtg ttgatcacga tcctcgaagg
     7261 cgcgctccat ctgaaccagt atcaggagaa ctggatcgcc tatcgctcca cgtgtgaatc
     7321 gctcaagcac gaaaagtacg tctacatcgc gaaagcatcg ccgtatgcag gcgtcgccga
     7381 tccgcgcgcc ctcctcgccg agcgcgtgga gtcgctcgtc tcccaggaac acgccaaatg
     7441 ggcgtcgtcg caacaggaga ataccaaggc gaaaccggcg acgacgtgat ccggtcgagc
     7501 catccaagtt ctatcgaatt tcgacctccg ccctgacagc actcgggcga ggacgcccga
     7561 gtgcccgcgg tcgagacgac cgcactccat gtagcgctga ggattg
//
LOCUS       DATLVW010000237         6054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_588289, whole genome shotgun sequence.
ACCESSION   DATLVW010000237 DATLVW010000000
VERSION     DATLVW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588289"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VNN25_07575"
     CDS             <1..976
                     /locus_tag="VNN25_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXH91422.1"
                     /translation="EQRAAEVEVAQTRLILSTSVASAYAGLLHLYTLRDNAADTLGLR
                     MQTVALFRQRHQFGLETLLSVRQVEARQAAAKGDLLVIDERIGLQRNAIAALLGAGPD
                     RGLEITRPKVAWAGSQGLPSNLSLDLLGRRPDIVAARLRTEAAAHRIDQRKAGFYPSV
                     NLLAFAGFQSVGIDNLFKSTSQSGAAGGAISLPIFNTDRLQGQFKGARAEYDAAVATY
                     NGTLSTALREVADAATSRMSLDQELAAARAAVAAAAEAHQLVSSRYEGALATYLDVLS
                     AEDSLISARRSEAELETRALILDVSLVRALGGGFTPDSSSAPTHNPQT"
     gene            1081..1974
                     /locus_tag="VNN25_07580"
     CDS             1081..1974
                     /locus_tag="VNN25_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH91423.1"
                     /translation="MFSLLGLAVAAAGGGAVEYWKLVGSRYVSTDNAYTAAEVAQVAS
                     ETEGSVMQVNVVDSQNVRRGDVLVMIDDTDAKLAMRQAQADLARAQAQVIAAISDVER
                     TNIDLTRREALVASGSVSGDELTNARNRASGARAALDAARAQVALARARLEKSDVDLG
                     RTVIRAPIDGVIARRQVQLGQRVQPSMPLLSVVPVDKLYVNANFKEVQLTKVHTGQSV
                     ELKSDLYHSKVVYHGVIEGFDGGTGAAFALIPAQNATGNWIKVVQRLPVRIRLNPEEL
                     AAHPLRVGLSMDAKVDLNSNS"
     gene            2061..3551
                     /locus_tag="VNN25_07585"
     CDS             2061..3551
                     /locus_tag="VNN25_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXH91424.1"
                     /translation="MLWTAGIVLALSNFVAVLNMTIANVTVPNIAGALGAGSSQGTWV
                     ITSYAVAEAITVPLTGWLSGRFGAVRLFCIAVVLFGLCSLLCGLSTSMPMLLGMRVLQ
                     GMAGGPLMALSQTLLLRIFPKRQSMQAMGLWAMTTLLAPVVGPVLGGWLCDQYSWPWV
                     FLINVPMALLFGVLAWVLLKRFHEPAVIKPVDKVGMLLLIVWVAALQIMLDEGKDLDW
                     FSSTEICVLAIVAVIGFLSFLIWELTQEYPIVDLRVFRHRGFSSCMLVLGLAFGAFFG
                     INVLTPQWLQYNMGYTTTWAGLVVAWGGVLSVVFSPVAAKLANRFDPRILIFIGCVWL
                     GLDTFWRAYATSDMSYWVIVVPLFFMGVGMPMYYVPLTGLAMGSVREEETASAAGLMN
                     FVRTISGAIATSLVTTSWQNRTIIAHAKLANVVDPTNQLAASLPPGTTGQMVREMFNN
                     LVTSQSLMLATNGLMMVIAVVFVAASIAIVLAPKAARTVDPASVGH"
     gene            3725..4867
                     /locus_tag="VNN25_07590"
     CDS             3725..4867
                     /locus_tag="VNN25_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH91425.1"
                     /translation="MDFPADVRFLSTDRASVEAKSTAALQRLSISSKDGMLRALVLSG
                     GGAGGAFGAGALVGLSRRQERPHYDLVTGVSTGALIAPFAFLGSEWDSQLTEAFTNGQ
                     GEQMSVHGLLALPLGASRRSAALTALVDRYVTPDLIRAVAKEAGNGRLLWVATTDLDK
                     EETVIWDLGAIAMRGGEPARKLFRDVVVASASIPGVFEPVLIHVQLGGRLYDEMHVDG
                     NVSASLFIAPAAAYFALLDQRSLNGARVYVLINGQIIDAPETTPFKLRPIVARTFSVA
                     LKHMSRAQVVAVDQFAEKYRMSVQSTYLPFDYPKYSSADFRASTMRPLFEYGVRCAQS
                     GRLWTTMDETMDTVAAAAPPSKRIAPVDQAVECPLRNDRFTADDRR"
     gene            4864..>6054
                     /locus_tag="VNN25_07595"
     CDS             4864..>6054
                     /locus_tag="VNN25_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXH91426.1"
                     /translation="MSESAERRGAGNGMSVTEATGWLRDHSSRIMTAGIILALLYLGR
                     SVLIPLALAIMLSLLVAPLIRALRRLRIGRASSVLVAVAVLTVSCVGVAAALGAQILH
                     IAESLPQYESNVQRKLKILEVVTIGPVLKLTNETSRLTGIRQSVEALPVPARDLERSP
                     PPEFGTHPLQLVWKLLTTVWRPVQFAGIVLLVLIFVLLEHESLRDRFIRIAGATDIRS
                     ATLALNDAGDRLSRYFVSQFAVNLVFGLAIWMSLSMLRLPQALLCGILAGVMRFVPYV
                     GVAIAGLFAALLALAVDPGWSLALSTLGVFLLLDIVVGQLVEPHLYGHATGLSPLSVV
                     VGAIFWSWLWGPAGLILSTPLTLCLMVAGRHMKGFGVLEVLLGNAQPLTLPQRFYQRA
                     LSEDP"
BASE COUNT         1031 a         1948 c         1858 g         1217 t
ORIGIN      
        1 ggagcagcgt gcggccgagg tcgaggtcgc acagacgcgg ctgatcctct cgacctcggt
       61 cgcctccgcc tacgcaggtc ttctgcatct ctatacgctg cgtgacaacg cggccgacac
      121 gcttgggctg cgtatgcaga cggtcgcctt gttccgccag cgccatcagt tcggccttga
      181 gacgctcttg agcgtgcgac aggtcgaggc acggcaggcg gccgccaagg gcgatctctt
      241 ggtgatcgac gagcgcatcg gcctgcagag gaacgcgatc gcggcgctcc tcggcgcagg
      301 ccctgatcgg ggcctcgaaa tcactcgtcc gaaggtcgcc tgggcaggtt cccagggatt
      361 gccttcgaac ctgtccctgg accttctcgg gcgtcgtcct gacatcgttg ccgcgcgact
      421 gcgcactgaa gccgccgcgc accgcatcga tcagcgcaag gccggattct acccgagcgt
      481 caacctgctc gcgttcgcgg gcttccagtc ggtcggcatc gacaacctct tcaagtcgac
      541 ctcgcagtcg ggtgctgcag gtggtgctat ctcactgccg atcttcaaca ccgatcgcct
      601 tcagggccag ttcaagggcg cacgcgcgga gtatgacgcc gccgtcgcca cgtacaacgg
      661 gacgctctca acggcgctgc gcgaagtggc cgatgcagcc acgagtcgca tgtccctcga
      721 tcaggaactc gccgccgccc gggctgccgt agccgccgcg gcggaagcgc accaactcgt
      781 cagctcccga tacgagggag cactcgccac gtatctcgac gtcctatcgg cggaggactc
      841 gctgatatca gcgcgccgct ccgaggcgga gctcgagacc cgggcgctga ttcttgacgt
      901 atcgctggtg cgcgcactgg gcggcggctt cacgccggac agctcgagcg ccccgaccca
      961 taacccccaa acctaaaaac cttcttaagg agacctgtca tggatatgca agcttcaatc
     1021 acccccgagt cgacgctcga cacgccgatg gccaaacccc gcaagaaaca gcgcgccgtg
     1081 atgttctcgc tgctcggcct cgccgtggca gctgccggcg gcggcgccgt cgagtactgg
     1141 aagctcgtcg gttcccgcta cgtgagcacc gacaatgcct acaccgctgc cgaggtggca
     1201 caagtcgcct ccgaaacgga aggatcggtg atgcaggtga acgtcgtcga cagccagaat
     1261 gtaaggcgcg gcgatgttct cgtcatgatc gacgacacgg atgccaaact cgccatgcgt
     1321 caggcgcagg cggatcttgc ccgcgcccaa gcgcaagtga tagcggccat ttcagacgtc
     1381 gagcgcacca acatcgacct gacacgccgt gaagcccttg tggcgtcggg ctcggtgtcg
     1441 ggtgacgagt tgaccaacgc caggaaccgt gcgagcggtg cgcgtgccgc actggacgcg
     1501 gctcgtgcgc aggtcgctct cgcccgcgcc cgcctcgaga agtcagacgt cgacctcggt
     1561 cgcacggtga tccgcgcgcc catcgatggc gtgattgcgc gccggcaagt gcagctcggt
     1621 cagcgcgtcc agccctccat gccgctcctg tccgtggtgc cggtcgataa gctgtatgtc
     1681 aacgcgaact tcaaggaagt acagctcacc aaggtccaca ccggacagag cgtcgagctc
     1741 aagtccgacc tctatcacag caaggtggtc tatcacgggg tcatcgaagg gtttgatggc
     1801 ggtaccggtg ccgcttttgc cttaatccca gcgcagaacg caaccggcaa ctggatcaaa
     1861 gtggtgcagc gtctgccggt gcgcatccgc ttaaatcccg aggagctcgc tgcgcaccca
     1921 ctgcgcgtgg ggctctcgat ggatgccaag gtcgacctga acagcaattc ctaaccctcg
     1981 aagtagatct taaggaacct gccatgacac atcccgccat cgccatcgat accaaggacc
     2041 cgcccgctct cgagggcggg atgctctgga cggccggcat agttctcgcg ctgtcgaatt
     2101 tcgtcgccgt gctcaacatg acgatcgcca acgtcaccgt gccgaacatt gccggcgccc
     2161 ttggcgcggg ctccagccag ggcacgtggg ttattacctc ttatgccgtc gccgaggcga
     2221 tcacggtacc gctcacgggc tggctgtccg gccgcttcgg cgcagtgcgg ctcttctgta
     2281 tcgccgtcgt gctgttcggc ctctgctcgc tcctttgcgg cttgtccaca tcgatgccga
     2341 tgctgctcgg tatgcgggtg ctgcagggga tggcgggcgg tccgctcatg gcgctatcgc
     2401 agacgctgct gctgcggatc tttcccaagc gccaatccat gcaggcgatg ggtctgtggg
     2461 cgatgacgac gctgctcgca cccgtggtgg gtccggtgct cggcggatgg ctgtgcgatc
     2521 agtactcgtg gccctgggtg ttcctgatca acgtacccat ggcgctgctc ttcggcgtgc
     2581 tcgcttgggt tctgctcaag cgctttcacg agcccgcggt catcaagcct gtcgacaagg
     2641 tcggcatgct cctcttaatc gtctgggtgg ccgcgctgca gatcatgctc gatgagggca
     2701 aggatcttga ctggttctca tcgaccgaaa tctgtgtact cgccatcgtt gccgtgatcg
     2761 gattcctctc cttcctgatc tgggagctga cccaggagta cccgatcgtc gacctgcgag
     2821 tcttccggca tcgcgggttt tcctcttgca tgctcgtgct cggcctcgcc ttcggggctt
     2881 tcttcggcat caatgtgctg acgccgcaat ggttgcagta caacatgggc tacaccacca
     2941 cttgggccgg actcgtggtg gcttggggcg gcgtgctctc ggttgttttc tcacccgtag
     3001 ccgccaagct cgcgaatcga ttcgatccgc gcattctcat tttcatcggc tgcgtctggc
     3061 tcggcctgga cacgttctgg cgggcctacg ccacctccga catgagctac tgggtgatcg
     3121 tcgttccgct cttcttcatg ggggtcggca tgccgatgta ctacgtgccg ctgaccggcc
     3181 tcgccatggg ctcggtcagg gaggaagaga ccgcctcggc ggcaggtctc atgaacttcg
     3241 tgcgcacgat ctccggtgcc atcgccacct cgctggtgac gacctcctgg cagaaccgca
     3301 cgatcattgc gcacgccaag ctcgcgaacg tcgtcgatcc cacgaaccaa ttagccgcct
     3361 cgctcccccc gggtacgacc ggacagatgg tccgtgagat gttcaacaac ctcgtgacga
     3421 gccagagcct gatgctcgcg accaacggat tgatgatggt gatcgccgtc gtgttcgtcg
     3481 ccgcctcgat cgcgatcgtg ctcgcgccca aggccgctcg cacggtcgac ccggcctcgg
     3541 tcgggcattg attcacagtt gcgcaataag gagtcaaggt atggtcacga cgcaaatcag
     3601 accccaccgc cgctggcagg caagggctct acgctggacg tgcgtgctaa gtatcttggc
     3661 gctggtggga tgtgccctcc cgcgccgtga cgcaccaccc accatattca gtgcggcaac
     3721 ccccttggat tttcctgccg acgtgcgttt cctcagcacc gaccgggcga gcgtcgaggc
     3781 gaaatccaca gccgctctgc agcgcttgag catttcatcg aaggacggaa tgctacgcgc
     3841 tctggtcctc tccggcggcg gtgccggcgg cgcgttcggc gcgggagcct tggttgggct
     3901 cagccgccgt caagagcgcc ctcactatga cctagtcacg ggcgtcagta ccggggcgct
     3961 catcgctccg tttgcgttcc ttggttccga atgggactca cagctgaccg aagccttcac
     4021 gaacgggcaa ggtgagcaga tgagtgtgca cggactgctc gcgttgccgc tgggtgcatc
     4081 gcgccgcagc gccgccctca cagcgctcgt ggaccgctac gtcactccgg atctcattcg
     4141 cgctgtcgcc aaagaggcag gcaatggacg gcttctgtgg gtggccacga ccgacctcga
     4201 caaggaagaa acggtcatct gggacttggg tgccattgcc atgcgtggcg gcgagccggc
     4261 tcgcaaacta ttccgggacg tcgtggtggc atcggcgagc attccgggtg tgttcgagcc
     4321 cgtactcatc cacgttcaac tgggggggag gttgtacgac gaaatgcatg tcgatggaaa
     4381 cgtatcggcc tcgctcttca tcgcgcccgc tgcagcctac tttgcattac tggatcagcg
     4441 gagcctcaac ggtgcccggg tctacgtgtt gatcaacggg cagattatcg atgcgcccga
     4501 gacgacccca ttcaagctgc ggccgatcgt ggcgcgcact ttctcggtgg cgctaaagca
     4561 catgtcgaga gcgcaggtag ttgcagtcga tcagttcgcc gagaagtacc gcatgtcggt
     4621 ccaatcgacg tatttgccgt tcgactatcc caaatacagc tcggctgact ttcgagcatc
     4681 cacgatgcgc ccgctgttcg agtacggggt gcgatgcgca cagagcggac ggttgtggac
     4741 caccatggac gaaacgatgg acacagtcgc ggccgccgcc ccgccctcga agcgaatcgc
     4801 gccggtggat caagccgtag agtgtccgct gagaaacgac agattcacgg ctgacgatag
     4861 gcgatgagcg aaagtgccga gcgtcgcggg gccggcaacg gtatgagtgt cacggaagcg
     4921 accggatggc tgcgcgatca cagtagccgc atcatgaccg cgggaattat cctggcgctc
     4981 ttatatctcg ggcggtccgt gctgataccg ctcgcgctcg ccatcatgct gagcttgttg
     5041 gtggcgccgc taattcgggc gctgcggcga ctacgaatcg gcagagcctc ttcagtgctg
     5101 gttgcggttg cggtactaac ggtgtcatgc gtgggtgttg cggccgcgct tggcgcgcaa
     5161 atcctgcaca tcgcggagag ccttcctcaa tacgaatcga atgtacagcg caagctcaag
     5221 attctcgagg tagtaaccat cggcccggta ctaaaactca ctaacgaaac gagtcgcctt
     5281 accgggatcc gtcaatccgt cgaggcgctc cccgttcctg cccgcgatct cgagcgctcc
     5341 ccgccacccg aattcgggac ccacccgcta cagcttgtat ggaagctcct gacaaccgtt
     5401 tggcgtccgg tccaattcgc aggcatcgtg cttctggtcc ttatcttcgt attgctcgag
     5461 cacgaatcac tccgcgaccg cttcatccgc atcgcgggcg cgaccgacat tcgctcggcc
     5521 actctcgcgc tcaacgacgc cggtgaccga ctgtccaggt attttgtgtc gcagttcgcg
     5581 gtcaacctcg tcttcgggct ggccatctgg atgagcttga gcatgctgcg cctcccgcag
     5641 gccctgcttt gcggaatact ggcaggtgtg atgcgcttcg taccctatgt gggtgtagca
     5701 atagccggcc tctttgctgc ccttctcgcc ctcgccgtcg atcccggttg gtccttggcg
     5761 ctgagcacct tgggtgtgtt cctcctgctc gacatcgttg tagggcaatt ggtggaaccg
     5821 cacctgtatg gtcacgcaac ggggctatcg ccgctctcgg tagtcgtggg agcgatattc
     5881 tggagctggc tctggggtcc ggccggcctg atcctgtcaa cgcctctcac gttgtgtctc
     5941 atggtcgcgg ggcgccatat gaaggggttc ggagtgctcg aggtgctact gggcaatgcg
     6001 cagccgttga cgttaccgca gaggttttac caacgggcgc tgtccgagga tccc
//
LOCUS       DATLVW010000238         9777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_420569, whole genome shotgun sequence.
ACCESSION   DATLVW010000238 DATLVW010000000
VERSION     DATLVW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420569"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNN25_07600"
     CDS             <1..609
                     /locus_tag="VNN25_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91427.1"
                     /translation="ARGTNISLYALDHGAVAARVYRLVAGTDVSLHFRRDGDGTQQGV
                     SAARVFGDSLELHAEIARRHALAGTQYTFKRNVNLVFELYHSGDGLDARAWRAFRLMA
                     DNARSETTIRTANGEYAPLRMARDYAFVRIDWPFEKLDAELIMITNMRDGSLLARATF
                     THKLRSNMSVYLIDTEFAGARNSELAYMQVRRVTTGGARLYF"
     gene            709..1818
                     /locus_tag="VNN25_07605"
     CDS             709..1818
                     /locus_tag="VNN25_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXH91428.1"
                     /translation="MNEQKMKLVGILTWIIVGIPTVLWEAQYRSLFTARAVLLFGAFI
                     VFIIGFFFATRPGCGNTIRISMLVIESLAAFTCVILQPSGFLAVLLVVIAGQLGAYPP
                     RYAVGGITINSIVLGFIIANNGGSGVIYALVYFAFSIFALFSMHVAHSEMEARKSLAE
                     ANAELRMTTELLEISSRTSERLRIARDLHDLIGHHLTALSLNLEVASHLSSGEAKEQV
                     EKSKGIAKQLLVDVREVVSRLRNDEPVDLTASLESLRDVMVAPSLHLDYQDGLAVADA
                     NVAQIALRTVQEIVTNAVRHSGARNLWLKLATGDRALSIDARDDGTGVDNIRFGNGLL
                     GLRERVEQIRGTFEVTSMRGRGFSVHVTLPLGGAA"
     gene            1815..2456
                     /locus_tag="VNN25_07610"
     CDS             1815..2456
                     /locus_tag="VNN25_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002807809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH91429.1"
                     /translation="MIRVCIVEDQTIVRQGLRSLLALVPDIEVAAEASDGEEAVAVIQ
                     REKPDVVLLDLRMPKLDGVGVLRQLRERGAARPTLILTTFDDDAILFEAVRAGAKGWL
                     LKDVSLERLTSAIRTLAEGGTCIEPVITERIMRALEKSDSSFEAADLPEPLTDRERTI
                     LRLLAGGYSNKEIGELLNITDGTVKNHISNLLAKLGVRDRTRAVLRAIDLGVI"
     gene            complement(2537..3253)
                     /locus_tag="VNN25_07615"
     CDS             complement(2537..3253)
                     /locus_tag="VNN25_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91430.1"
                     /translation="MPTKNRNGNRSSKTAVQKRTRTKPKAIAAIPDPVAAAARMVELL
                     NEMEAVIPDLIQPDITKITRIAQGAKFGRALIPVMINAVNSYPPFQQRNMFDSAAAQY
                     ALDYESQMHPITKRMAAITMAMSYSMDQKLSVSGEQALQLFQWGKRHVKQPDGGGARI
                     YVDEMQSVVDRTMNRRKRLKPANNPPTTPPSTTPPTTPPTQGLLAPSLAAKAAAKAQT
                     DTDDAEMLRLVHKIATDTKK"
     gene            4199..4492
                     /locus_tag="VNN25_07620"
     CDS             4199..4492
                     /locus_tag="VNN25_07620"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH91431.1"
                     /translation="MRKEILTTKNIQGSPDLLIEILSESNRKYDEVTKRALYERTGVP
                     EYWIVDPVADAIRIFRRNAAGRYERAGELTSDDTLTSPLFPTLEIRLDRIFAD"
     gene            complement(4635..4823)
                     /locus_tag="VNN25_07625"
     CDS             complement(4635..4823)
                     /locus_tag="VNN25_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91432.1"
                     /translation="MAEQTAAPGGVRGKYYASYKAGTNLVLLAPDVAEVFRDSTSVNA
                     ALRQFLAEHGAPPGKTAG"
     gene            complement(4980..6437)
                     /locus_tag="VNN25_07630"
     CDS             complement(4980..6437)
                     /locus_tag="VNN25_07630"
                     /inference="COORDINATES: protein motif:HMM:NF039735.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6348 family protein"
                     /protein_id="HXH91433.1"
                     /translation="MDEGLLAHIRKHRDELLAIHHYFWKFLARVPPSRHNIHYLILRG
                     ALRRSYSLTRAFFELVEAENFVSAAPLVRLELDNGLRVNLVALCGDGHKVLVHLAAGK
                     RLDKLKDADGNRLTDRFVVNALTGLFPWLPRLYSEGSRFVHFSDVHIFAFDESQRDLA
                     DRDTFFSEVAYIELVESFALAAKFSYDFVARYRTKCLGEWRAILPKLQRRTQPPHARG
                     HLNAKALRDLSPRVRQRQDKARRELIAQPDALDASLARILFAHGLLTCKLQSGWNVVD
                     GRFPAIRTRLLNQRAVGHRVALQLDVEVLLPDATLRESFMGIGDNRAAAAQDAMRNFG
                     IGSLHVVLAGLWDRTDHSQVDVKKWSIGKSTWRVYQGHFTIKCHDVASIQLPFELDSQ
                     LEALLCSRELPGTLHWFRMFFGVVNRTRITLEALLDNERWDEAEAAIGRLPWNVTQDY
                     FSVRAFYILRRDHEHEELWSGFRTTDDADTADDIA"
     gene            complement(6483..8015)
                     /locus_tag="VNN25_07635"
     CDS             complement(6483..8015)
                     /locus_tag="VNN25_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXH91434.1"
                     /translation="MSSAAKVGVFMLIILAILGYFILKIEDVQIGHGTGARKVTALFD
                     SVAGLDNKSAVRVAGVRVGKVSNIKLRPDGKAEVTLDIDPDVVLHPGAVAHVANLGLL
                     GEKYVELDPGPSNAPVIPQEQSVVLHGTQPASMDDITNQVAAIANDVKAITASLRGAM
                     SGPAGQQRLVDIVENVRTITEQVRDLIAANRTNVDPTVANARVISESLRVEIPRLAAS
                     IDRMATQMNGTVGENREDVRHIVENLRTLSADLKTTSDNLNAVTAQVRSGEGTVGKLL
                     YSNEAHDRLTAALGSVESGVNELRNTLGRASRMQLDLGLNGDYYAGLPAQQDGSDKLG
                     NSRSAVWLRLNPNPEHNRFYNIEVADTPYGKRRDKTIEETVTNPATGQSQTTITQQTR
                     FDRSFLISAQAGWTIAPFAVRLGMIDSSGGAGVDYFFNNRIRATGEVFDFGHRNGDNN
                     PHVRLFGEYVFRQEKPTTPTIFIRSGVDNVLNHTAFTFGGGIRWRDDDLKYLLGSVPI
                     PK"
     gene            complement(8221..8823)
                     /locus_tag="VNN25_07640"
     CDS             complement(8221..8823)
                     /locus_tag="VNN25_07640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXH91435.1"
                     /translation="MSFILASQSPRRRELLTSIGLAFDVIPSDVPEIHQPGESPEEYV
                     ARLSRDKAEVVAASHPEAWIIAADTTVLLGDQLLEKPVDANDARRMLGLIAGKTHVVY
                     SGVTLQNTARDWRETRVAESEVRMLPLDEREIAWYVATGEPLDKAGAYAVQGIGGMFI
                     DSIHGSYTNVVGLPLALLFEMLRRAGIDLVQELGIGRRAL"
     gene            complement(8820..9071)
                     /locus_tag="VNN25_07645"
     CDS             complement(8820..9071)
                     /locus_tag="VNN25_07645"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH91436.1"
                     /translation="MWNDRLRFAERVEKDWRALTDDQRAVTARALETIDEDPIIGAPL
                     FEPLRGYWSYRSEGIRIIYRIVTEARFIVVLSISLVLAR"
     gene            complement(9244..9504)
                     /locus_tag="VNN25_07650"
     CDS             complement(9244..9504)
                     /locus_tag="VNN25_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91437.1"
                     /translation="MDKRFDILDVSEDLRALVGECELTGNRTTFLRNERPIAILISHD
                     EYLALRETIDIADDAPLRAAIEEGETEVLRGAMLAAEDLLGG"
     gene            complement(9504..>9777)
                     /locus_tag="VNN25_07655"
     CDS             complement(9504..>9777)
                     /locus_tag="VNN25_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HXH91438.1"
                     /translation="ILPPDVFPLLEKTERGAGGEIQLTDALRELVRTGDFFGYVFEGK
                     RYDAGEKLGYLKATVDYALRHPAVGKEFRTYLESLFGNDVPKNRQN"
BASE COUNT         1831 a         2880 c         3045 g         2021 t
ORIGIN      
        1 gcccgaggga cgaacatctc gctctatgcg ctcgatcatg gcgccgtcgc cgcgcgcgtt
       61 tatcgactcg tcgcaggcac cgacgtatcg ctccacttcc ggcgcgatgg cgatggcacg
      121 cagcagggtg tgagcgctgc acgcgtgttc ggcgattcac tcgagctgca tgcagagatt
      181 gcacgccgcc acgcgctcgc cggaacgcag tacaccttta aacgaaacgt caatctcgtg
      241 ttcgagctgt atcacagcgg cgacggactc gacgcgcgcg cgtggcgtgc atttcgcctc
      301 atggccgaca acgccaggag cgaaacgacg atccgcacgg ccaatgggga gtacgctccg
      361 ctgcgaatgg cccgcgacta cgcgttcgta cgcatcgact ggccgttcga gaaactcgac
      421 gccgagctga tcatgatcac gaatatgcgc gatggttcgc ttctggctcg tgccacgttc
      481 acgcacaagc ttcgatcgaa catgagcgtc tatctgatcg acaccgagtt cgctggtgca
      541 cgcaacagcg agcttgccta catgcaggtg cggcgtgtga cgacgggcgg ggcgcgacta
      601 tatttttgaa gacaaaaggc atcaccacag agacacagag gacacagagt tacctctgtg
      661 atctcagtgt ctctgtggtg atgggtttca cgacctaatg cgatgaacgt gaacgaacag
      721 aagatgaagc tggtcggcat tctgacgtgg atcatcgtcg ggatacccac cgtgctttgg
      781 gaagcgcagt accgctcgct cttcacggcg cgcgcggtgc tgcttttcgg cgcgttcatc
      841 gtcttcatca tcggcttctt ctttgcgacg cggcccggtt gcggcaacac catccgcatt
      901 tcgatgctcg tcatcgagtc gctggcggcg ttcacctgcg tgattctgca gccgtccggc
      961 ttcctggctg tgctgctcgt ggtcatcgcg gggcaactcg gcgcatatcc gccccgctat
     1021 gccgtcggcg gcatcaccat caactcgatc gttctcggat tcatcatcgc gaacaacggc
     1081 ggttcgggcg tcatctacgc gctggtttac tttgccttct cgatcttcgc gctcttctcg
     1141 atgcacgtgg ctcattccga gatggaggcg cggaagtcgc tggccgaggc gaatgcggag
     1201 ctgcggatga ccacggagct gctcgagatc agcagccgca cctcggagcg ccttcgcatc
     1261 gcccgcgatc tccacgacct catcgggcat cacctgaccg cgctcagcct caatctcgaa
     1321 gtggccagcc atctctccag cggggaagcg aaagagcagg tcgagaagag caagggaatc
     1381 gcgaaacagt tgctcgtcga tgtgcgcgaa gtcgtcagcc gcctgcgcaa cgacgagccg
     1441 gtcgatctca cagcctcgct ggagtcgctg cgcgatgtga tggtcgcgcc ttcgctccac
     1501 ctcgactatc aggacggcct cgcggtcgcc gatgcgaatg tcgcgcaaat agcgctgcgt
     1561 acagtccagg agatcgtgac gaatgccgtg cgccactccg gcgcgcgcaa tctctggctg
     1621 aaactcgcca ccggcgaccg cgccctctcg atcgatgccc gtgacgacgg caccggcgtc
     1681 gacaacatcc gcttcggcaa cggtctcctc ggactccgcg agcgcgtcga gcagattcgc
     1741 ggcacgttcg aggtgacatc aatgcgcgga cgcggtttct ccgttcacgt gaccctcccc
     1801 ctcggcggtg ccgcgtgatc cgcgtctgca tcgtcgagga tcagacgatc gtccggcagg
     1861 gtctgcgcag cctgcttgca ctcgtgcccg acatcgaagt ggccgccgag gcgagcgatg
     1921 gggaggaggc ggtggccgtc atccagcgcg aaaagccgga tgtcgtgctg ctcgatttgc
     1981 gcatgccgaa actcgacggc gtcggtgttc tccgccagtt gcgcgaacgc ggcgctgcgc
     2041 ggccgacgct catcctgacg acgttcgacg acgatgccat cctcttcgaa gccgtccgcg
     2101 ccggcgcgaa gggctggctg ctcaaagacg tgtcgctgga gcgcctgacc agcgcaatcc
     2161 gaacgctggc cgagggaggc acgtgcatcg agccggtcat caccgaacgg atcatgcgcg
     2221 ccctggagaa gagcgactcg tcgttcgaag cggccgacct ccccgaaccc ctcaccgacc
     2281 gcgaacgaac gatcctccgc ctcctcgccg gcggctacag caacaaagag atcggcgagc
     2341 tgctcaacat caccgacgga acggtgaaga atcacatctc gaacctgctg gccaagctcg
     2401 gcgtgcgcga ccggaccaga gcggtcttgc gggcgatcga tctgggcgtg atttgaggga
     2461 atggtgagcc gcccaacccg gcaactgcga aagggtgccg cgttctcatt cacgcggcac
     2521 cccatctcac gcgcggctac ttcttcgtat ccgtggcaat cttgtgaacg agacggagca
     2581 tctcagcgtc atccgtatcg gtttgcgcct tcgccgcagc cttcgcagca agagaggggg
     2641 ccagaaggcc ctgcgtggga ggagtggtgg gaggagtcgt tgagggtggt gtggtgggag
     2701 ggttgttggc gggtttcagc cgcttgcggc ggttcatcgt ccgatcgacg acactttgca
     2761 tttcatccac atagatccgc gccccgccgc cgtcaggctg cttcacgtga cgcttgcccc
     2821 attgaaacaa ctgaagggcc tgttcgccgg agacggacag cttctgatcc atcgaatacg
     2881 acatggccat cgtgatcgcg gccatcctct ttgtgatcgg gtgcatctgg ctctcgtagt
     2941 cgagggcgta ctgcgcggcg gcggagtcga acatgttgcg ctgctgaaac ggtggatagg
     3001 agttgacggc gttgatcatg accgggatga gcgcgcgtcc gaactttgcg ccctgggcga
     3061 tgcgtgtgat cttggtgata tccggctgga tcagatcggg gatgacggcc tccatttcgt
     3121 tgagcaactc gaccatcctg gccgctgccg cgacagggtc ggggatggcc gcgatggcct
     3181 ttggtttggt cctggtgcgc ttctgaacgg cggttttgga agagcggttt ccgttgcggt
     3241 ttttcgttgg catccctgtc tcctttggcc gcacagtcga ggtccagcct cctgcgggct
     3301 catcttctga gacgagtcac gaatggaaag gttcctggga gtgaatgcga tcggtttcgg
     3361 cggggtctcg atctctgccc agcaggtcac gatctgccct gatcgacacg cgatctacgc
     3421 ctggtagcta tggatctatt ctcggtagat cgcgatctac gtttggtaga tgcggatctg
     3481 tttttgggag atgtggatct ctttttaaga gatgcggatc tgtttttgag agatgcggat
     3541 ctcttttttt agatgcggat ctgtttttag gagatgcgga tctgttttca agagatgcgg
     3601 atctcttttt aggagatgcg gatctgtttt tgggagatgc ggatctcttt ttctagatgc
     3661 ggatctgttt ttaagagatg cggatctgtt ttaaagagat gcagatctct ttttttaaga
     3721 tcgcgatcta tcctgggggg cgacgtctga tctcggtaag tgcattcttg atcaatggct
     3781 tacgcccaag cacaccgtca gctcttctga gagtcgtgtc atcctcagcg ggcgaacgcc
     3841 gcaggcgccg gtgaagcgga gacgacactc tgtctgacga gctcggaatc atgtcaatgg
     3901 cgtaagttca tgaatctaaa ggcgaacgat gcgctcacgc aaatcgtgtc atcctgagcc
     3961 gccgaagccg ccgcaaggtt cgccgctctc gcaacccggc agacatcggc ggcggaggac
     4021 ggtcgaagga tctcaaaatg cgaagatccc gttcgagcgt cacgacccgc tcgcactgaa
     4081 cgaacacgac ttgcgaagtc tgagatcctt cgacgtcctg cgccgccgag cacgtactgc
     4141 gcaaacatcg agattcgcgc gggcggctcc ggcagtctca ggatgacacc aatctcgatt
     4201 gcgcaaggag attctcacga cgaagaacat ccagggatcg cccgatctcc taatcgagat
     4261 cctgtccgag tcgaatcgga agtacgacga agtgaccaag cgcgctctct acgaacgcac
     4321 cggcgtcccc gagtattgga tcgtcgatcc ggtagcggat gccattcgga tcttccggcg
     4381 caacgcagcc ggacgctacg aacgcgcagg tgagttgacc agcgacgaca cgctcacatc
     4441 accactcttc cccactctcg agatccgcct cgaccgcatc ttcgcggatt agcttcgtag
     4501 ctggcccgtc ggaagaagat tctcgcggga ggagcgggtc tacgtaggtc ggactctcca
     4561 gtcctacttt ctcgtttcct ctgtcggact ggagagtccg acctacgtca cgtccgcagc
     4621 aaacactccc aaccctaacc tgccgttttc cccggaggtg caccatgctc tgcgagaaac
     4681 tgcctgagcg ccgcgttcac ggacgtggag tcgcgaaaaa cttcagctac gtctggagcg
     4741 agaagcacca gattcgtgcc tgccttgtag ctggcgtaat acttcccccg aacccctccc
     4801 ggagccgcgg tttgctcggc gatttcgaga tcttgtcgtt atccggattc aatagagagt
     4861 cttcgttcta ctgtcgttgc gttgcgcgcc ggccttatcc ggatccggtc ttcgcgtttg
     4921 gaatgagtcg cgatgaccaa atctgtgcct ctcagtgtac gtgagtatcc gagcaggagt
     4981 caggcgatgt cgtcggccgt gtcggcgtcg tcggtcgtcc ggaacccgga ccaaagttcc
     5041 tcatgttcat ggtcacgccg aaggatgtaa aaggcgcgaa cagagaaata gtcttgggtt
     5101 acgttccacg gaaggcgccc aatggcggcc tcggcctcgt cccaccgctc gttgtctaat
     5161 agggcctcca atgtgattct ggttcgattt acaacgccaa agaacattcg gaaccagtgg
     5221 agtgtgcctg gtagctcacg actacacagg agagcttcca attggctatc gagttcgaat
     5281 ggaagctgga tcgacgcgac atcgtgacat ttgatggtga agtggccttg atagaccctc
     5341 catgtcgatt tacctattga ccacttcttg acgtcgacct gtgaatgatc agtccgatcc
     5401 catagacctg ccagaactac gtggagcgag ccgatgccga agtttcgcat cgcatcctga
     5461 gcggccgccg cgcggttatc tccgatcccc atgaacgatt ctcgtagcgt cgcgtcgggt
     5521 aacagtactt ctacgtctaa ttgtagcgcg acgcgatggc cgactgcgcg ctgattgagc
     5581 aaccttgttc gaatggccgg aaagcgccca tcgacaacgt tccagccgga ctgcaatttg
     5641 cacgtcagga ggccgtgagc gaaaagaatg cgtgcgagcg acgcgtcgag tgcgtcgggt
     5701 tgtgcaatca attcacgacg cgctttatcc tgccgttgtc gaactcgcgg cgacagatct
     5761 cggagtgctt tcgcatttag atgaccgcgt gcgtgcggcg gttgcgttcg ccgctgtaat
     5821 ttcggaagaa tcgcacgcca ctcgccaaga catttggtgc ggtagcgagc cacgaagtcg
     5881 taggagaact tcgccgctag cgcgaaggat tcaaccaact cgatataagc aacttcagag
     5941 aagaatgtgt cgcggtcggc taagtcgcgt tgggactcgt cgaacgcgaa gatatgaaca
     6001 tcggaaaagt gcacaaacct agaaccttcc gaataaagtc gcggcaacca cggaaacaag
     6061 ccagtcagag cattcaccac gaagcgatcg gtaaggcgat ttccatcagc atccttcagt
     6121 ttgtcgagtc gcttgccggc agcgagatgc actagcactt tgtggccgtc accgcagagc
     6181 gccacgaggt tgacgcgtaa gccgttatcg agctcaagtc gcaccaaggg tgcagccgaa
     6241 acaaagttct ccgcttctac cagctcgaaa aaggcgcgtg tcagcgaata ggagcggcgg
     6301 agagcaccac gcaggataag gtaatgaata ttgtgacgtg atggcggtac ccgcgcgagg
     6361 aacttccaga aatagtgatg gatcgcgagc agttcgtcgc gatgctttct gatgtgagct
     6421 agtagtcctt cgtccacaga gcgctattgt ctccgagaat gagcttcttt acaggacacc
     6481 ccctacttag gaatcggaac actccccagc aaatacttca agtcatcatc tcgccacctg
     6541 atcccgcctc cgaatgtgaa ggcggtgtga ttgaggacgt tgtcgacgcc gctgcggatg
     6601 aagatcgtgg gggtcgttgg cttttcctgg cggaagacgt actcgccgaa gaggcggacg
     6661 tgggggttgt tgtcgccgtt gcggtggccg aaatcgaaga cttcgccggt ggcgcggatg
     6721 cggttgttga agaagtagtc gacgcctgcg ccgccgctgg agtcgatcat gccgaggcgg
     6781 acggcgaagg gggcgatcgt ccagccggct tgggcggaga tgaggaacga gcggtcgaag
     6841 cgggtttgct gcgtgatggt ggtctgcgac tggccggtcg ccggattcgt cacggtctct
     6901 tcgatcgtct tgtcgcggcg cttgccgtac ggtgtatcag cgacctcgat gttgtagaag
     6961 cggttgtgct cggggttcgg gttcaggcgc agccagacgg cggagcgcga gttgcccagc
     7021 ttgtcgctgc cgtcctgttg cgcgggcagg ccggcgtagt aatcaccgtt gaggccgagg
     7081 tcgagctgca ttctgctggc gcggccgagc gtgttgcgca gctcgttcac gccgctctcg
     7141 acggagccga gggccgcggt caggcggtcg tgcgcttcgt tgctgtagag aagcttgccg
     7201 actgtgccct cgccgctgcg aacctgcgcg gtcaccgcgt tgaggttgtc ggacgtggtc
     7261 ttgagatcgg ccgagagcgt gcggagattt tcgacgatat ggcggacgtc ttcgcgattc
     7321 tcgccgacgg tgccgttcat ctgcgtggcc atgcggtcga tcgaggcggc cagccgcggg
     7381 atctcgacgc gcagcgattc gctaatgacg cgggcgttgg caaccgttgg gtcgacgttc
     7441 gtccggttcg cggcgatcag gtcgcgtacc tgctctgtaa tggtgcggac gttctcgacg
     7501 atgtcgacga gacgctgctg cccggcgggt ccgctcattg cgccgcgcag cgatgcggta
     7561 atcgccttca catcgttcgc gatcgcggcg acctggttgg tgatgtcgtc catcgaggca
     7621 ggctgcgtcc cgtgaagcac gacgctctgt tcctgcggga ttaccggcgc attggacggg
     7681 ccgggatcga gctcgacgta cttctcgccg aggagtccaa ggttggcgac gtgcgcgacg
     7741 gcgccgggat ggagcaccac gtcgggatcg atatccagcg tcacttcggc cttcccatcc
     7801 ggacgaagct tgatgttcga cacctttccg acgcgcacgc cggccacgcg gaccgctgac
     7861 ttgttatcga ggccggcgac ggagtcgaag agcgccgtca ccttgcgcgc gccggttccg
     7921 tgaccgatct gaacgtcttc gatcttcagg atgaagtacc cgaggatcgc caggatgatg
     7981 agcatgaaaa cgccgacttt ggctgcggag gacatgggtt tgttgggtgt cgggagtcgg
     8041 gggtggggag tcggagaacc gttcccgcat ccaagcctca cttcatacgt cccgttcctt
     8101 tctcagcatc agttgtatcc gggggtcgcg cgtcgggtgt cgggtgtcgg agcgagtcgt
     8161 ggcgaaacgc ttggctagct cccacacccc acaccctact ccccactccc cgcgaaatct
     8221 ttacaacgcc cgtcgcccga tccccagctc ctgcaccaga tcgatccctg cccgtcgaag
     8281 catctcgaaa agcaaagcaa gtggtaatcc aactacgttc gtgtaactgc cgtggatgga
     8341 gtcgatgaac atcccgccaa tgccttgtac ggcgtaggcg cccgctttgt cgagcggttc
     8401 gccggtggcg acgtaccagg cgatctcgcg ctcgtcgagt gggagcatac gcacttccga
     8461 ctcggccacg cgcgtctcac gccagtctcg agcggtgttc tgcagcgtga caccgctgta
     8521 gacgacgtgc gtcttgccgg cgatcaagcc gagcattcgt ctggcgtcat ttgcgtcgac
     8581 cggcttttcg agcaattgat cgccgaggag gacggtcgtg tcggcggcga tgatccaggc
     8641 ctcgggatgg ctggcggcaa ccacctcggc cttgtcgcgc gagagtcgcg ccacatactc
     8701 ctcgggtgat tcgccgggct gatgaatctc gggtacatcg ctcggaatga cgtcgaaggc
     8761 caggccgatc gacgtcagca gctcgcggcg gcgcggcgat tgcgaagcga ggatgaagct
     8821 catcgcgcca ataccaagct gatactcaga acgacgatga agcgcgcttc ggtgacgatg
     8881 cggtaaatga tgcggatgcc ttcgctgcga tagctccagt aaccgcgcaa cggctcgaag
     8941 agcggtgctc cgatgatggg atcttcgtcg atcgtctcga gcgcgcgggc ggtgacggcg
     9001 cgctggtcat cggtgagcgc gcgccagtcc ttttcaacgc gctcggcaaa acggaggcgg
     9061 tcattccaca tgagcgaagt ctactcgcga agggattttg aaggtcgcca ccgagccggt
     9121 ggcggtacag ccggcgggcc gccggcgcta cgcttgccta cgcgcgtcga ggagtccaca
     9181 ccaacgcagg ccggtgtagc gacgccggct cggcggctgt atcgccggcg gcccgccggc
     9241 agttcatcca ccgagcagat cttccgccgc cagcatcgca ccgcgcagca cctcggtctc
     9301 cccctcttcg atcgcagcac gaagcggcgc gtcgtcggcg atgtcgatcg tttcgcggag
     9361 ggcgaggtat tcatcgtgcg agatgaggat ggcgatggga cgttcgttgc gcaggaacgt
     9421 ggtgcggttg ccggtcagct cgcattcgcc gaccagcgcg cgaaggtctt ccgagacatc
     9481 gagaatatcg aatcgcttgt ccattagttc tgacggtttt tcggaacgtc gttcccgaag
     9541 agcgactcca gatacgtgcg aaactccttg ccgacagccg gatgacgcag cgcgtagtcg
     9601 acggtggctt tgagatagcc caacttctcg ccggcgtcgt agcgcttgcc ctcgaagacg
     9661 tagccgaaga aatcgccggt gcgcaccagc tcgcgcagcg cgtcggtgag ctggatctcg
     9721 ccaccggcac cgcgctcggt cttctccagc agcgggaaga cgtccggcgg caggatg
//
LOCUS       DATLVW010000239         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_91720, whole genome shotgun sequence.
ACCESSION   DATLVW010000239 DATLVW010000000
VERSION     DATLVW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91720"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VNN25_07660"
     CDS             <1..192
                     /locus_tag="VNN25_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91439.1"
                     /translation="NRRKPRKPATNPPTTPPSNTPPTTPHIQGLMAPSLASRAKAQAD
                     QADDQILDLVHEIARDPKK"
     gene            complement(459..683)
                     /locus_tag="VNN25_07665"
     CDS             complement(459..683)
                     /locus_tag="VNN25_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXH91440.1"
                     /translation="MDYQDIITVERDKRGGQPCIRGLRITVYDVLSYLASGMSEEAII
                     SDYPDLTHEDILASLSYAADRERKTAVSHA"
     gene            complement(692..1081)
                     /locus_tag="VNN25_07670"
     CDS             complement(692..1081)
                     /locus_tag="VNN25_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91441.1"
                     /translation="MKIKREPTRLEKKARRGQRGYPVATIAFYGPDASRASKVAVGIV
                     RTEDGWPTEMKKWFSETGDVRQDAAIGDEVIAFIEEHGGKTFVLSPGIMGCPHEEGID
                     YPGGEVCPQCPYWANRDRLSGEELPVQ"
     gene            complement(1093..1437)
                     /locus_tag="VNN25_07675"
     CDS             complement(1093..1437)
                     /locus_tag="VNN25_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HXH91442.1"
                     /translation="MIIWVDAQLSPALAPWLTKQFGVDAVSVARLGLRDAKDPQIFAA
                     AREANAIVLTKDDDFVILLERLGPPPRVLWVTCGNTSNEHLRQVLTATLSDALELINA
                     GEVLVEIRDIAK"
     gene            complement(1434..1658)
                     /locus_tag="VNN25_07680"
     CDS             complement(1434..1658)
                     /locus_tag="VNN25_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXH91443.1"
                     /translation="MNQLAERITVDPEQCGGRPCVRGMRIRVSDVLDLFAAGLSAAQI
                     LEEMPDLEAEDLAACLRFASRYVDHPVLVA"
     gene            1780..>3310
                     /locus_tag="VNN25_07685"
     CDS             1780..>3310
                     /locus_tag="VNN25_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH91444.1"
                     /translation="MKIHLVSLEDGITATGFRKFAAYAEKINPDTRTFYVGTQRYRSM
                     WKSFVRKVGEGRAFGAPEVEEIAQELAQADVVAFSSMTGYADLTKELATRVRAINPKA
                     YTMWGGIHPIIYPEDAITADVDAICTGEGELAFAEWLYHFENGSDFQKVSSFWFKDRA
                     TGEIQRNPFRPLMSSAELASMPYPKYGGGEGIYQPDKGFRPVTRGDYLANNGLAYPAV
                     WSIGCPLHCTYCGNTVFIANDPEYRKIRHSGVDYIIGEVQAALKVHPYLKTVLFYDDS
                     FLAISLRELTEFATRWREEVGIEFCIYGIIPTYVQRDKVEILTWAGMNRIRMGVQSGS
                     ERILKFYKRPTPISRIEQAAAVLAEFAPYQINPSYDIIVDNPVETRQDVIDTLELVYR
                     LARPFTLNIFSLRVIPNTTLEQQMIAEGFDVEQINENYTALRPTWANVLLYILMVWKP
                     SRKTFDRWLLKVRAYNEPQPSHPILILFARIPWLIQQGVRHLKHGEFSVITGYPGYIL
                     WR"
BASE COUNT          680 a         1053 c          952 g          625 t
ORIGIN      
        1 aaccgccgca agccccggaa acccgccacc aaccctccca ccacaccacc ctcaaacact
       61 cctcccacta ctcctcacat acagggcctc atggccccct cccttgcctc ccgggcgaag
      121 gcgcaagccg atcaggccga cgatcagatc ctcgatctcg ttcacgagat cgccagagat
      181 ccgaagaagt agccgcgcgt tagacggggc gccgcgtgaa tgaaaacgcg gcgccccctt
      241 tcgcggttgg gggatcgcgc gaaaccccga gcgatcaatg tcgcgaagcg aatgcaagcc
      301 cttttcggta gtgggtcaat ttgaaaaccg atcaccactg agacacagag gtcacagaga
      361 ttgcctctgt gttctctgtg tctccgtggt tgcctttgcg ttggcgacca cgatcagcct
      421 ccaagttgac ccattgcggt tgtgatcgaa gagctgtctc acgcgtgcga gactgctgtc
      481 tttcgctcgc gatcggccgc atagctaagg ctcgcgagga tgtcctcatg cgtcaggtcc
      541 gggtagtccg aaatgatcgc ttcctcgctc attcccgagg cgagatacga gagaacatcg
      601 taaacggtga tgcgcaggcc acggatacac ggctgccctc cccgcttgtc tcgctcaacg
      661 gtgataatgt cctgatagtc cacgcgagca cctactgaac cggaagttcc tcgccgctca
      721 accgatcgcg gttcgcccag tacggacact ggggacaaac ttcgccgccg ggatagtcga
      781 tcccttcctc gtgcggacat cccatgattc ccgggctcag gacgaacgtc ttcccaccgt
      841 gctcttcgat gaatgcaatg acctcgtcac caatcgccgc atcctgccga acatcgccgg
      901 tctcggagaa ccacttcttc atctccgtgg gccagccatc ctcggtccgg acaatcccga
      961 cggcaacctt cgaagcccgc gaagcatccg gcccatagaa agcaatcgta gccacgggat
     1021 acccccgctg cccccgcctc gccttcttct ccaaccgggt cggttcacgt ttgatcttca
     1081 tgattgtcaa ttctatttcg cgatgtcgcg gatttcgacc aggacctcgc cggcgttgat
     1141 caattcgagt gcgtctgaca gcgttgccgt gagaacctgc cgaagatgct cgtttgacgt
     1201 gttgccacac gtgacccaaa gaacccgagg cggcggtcct agacgctcca gcagaatcac
     1261 gaaatcgtcg tccttggtca gcacgatcgc gtttgcttct cgtgcggcgg cgaagatctg
     1321 aggatctttg gcatcacgca acccgagcct ggcaacgcta accgcgtcaa caccgaactg
     1381 cttcgtaagc cacggcgcaa gtgcgggcga aagctgagcg tcgacccaga tgatcaagca
     1441 accaacaccg ggtgatcaac atagcgcgag gcaaagcgca agcacgccgc gagatcctcg
     1501 gcttcgaggt ccggcatttc ctcgagaatc tgagcagcgg aaagcccagc tgcgaagaga
     1561 tccagaacgt cactcacgcg aatgcgcata ccgcgaacgc acggcctgcc gccgcactgc
     1621 tcggggtcaa ccgtaattcg ctcggcaagc tggttcatgg atgcgattat acaaggcgag
     1681 gaacgcgctc ttgcgtgagc agcttttcgc acgatgaggc gccaggcgcc aaattcggaa
     1741 taaccccctc acctgctacg cttcgtggca cgtaacctga tgaagatcca tctcgtatct
     1801 ctcgaagacg gcatcacggc gaccgggttc cggaagtttg cggcgtatgc ggagaagatc
     1861 aatccggaca cgcggacttt ctacgtcggg acgcagcggt atcggtcgat gtggaagtcg
     1921 ttcgtgcgga aggtcggcga agggcgggcg tttggagcgc ctgaggtcga ggagattgcg
     1981 caggagctgg cgcaggcgga cgtcgtcgcg ttctcgtcga tgaccgggta cgccgatctg
     2041 accaaggagc tggccacgcg cgtgcgcgcg atcaatccga aggcgtacac gatgtggggc
     2101 gggatccatc cgatcatcta tcccgaggat gcaatcacgg cggatgtgga cgcgatttgt
     2161 accggtgagg gagagctggc ttttgccgag tggctctatc acttcgagaa cggcagcgac
     2221 ttccagaaag tcagcagctt ctggttcaag gatcgggcga ctggcgagat ccagcgcaat
     2281 ccgttccggc cgctgatgtc gtcggcggag ctcgcctcga tgccgtatcc gaaatatggc
     2341 ggcggtgaag gcatctacca gccggacaag ggatttcgtc ccgtcacgcg cggcgactat
     2401 ctcgccaaca acggccttgc ctatcccgcg gtctggtcga tcggctgtcc gctgcactgc
     2461 acgtactgcg gcaacaccgt cttcatcgcc aacgatcccg agtaccgcaa gatccggcac
     2521 agcggcgtcg actacatcat cggcgaggtg caggcggcgc taaaggtcca tccctatctc
     2581 aagaccgttc tcttctacga cgacagtttc ctggccattt cgctgcgcga gctgaccgag
     2641 ttcgccacgc gctggcgtga ggaagtaggc atcgagttct gcatctacgg gatcatcccg
     2701 acttacgtgc agcgcgacaa ggtcgagatc ctgacgtggg ccggcatgaa ccgcatccgc
     2761 atgggagtgc agagcggcag cgagcggatc ctgaagttct acaaacggcc gacgccgatc
     2821 tcgcgtatag agcaggccgc ggcggtgctg gcggagttcg cgccgtacca gatcaatccg
     2881 tcgtacgaca tcatcgtcga caaccccgtc gagacgcggc aggacgtgat cgacaccctg
     2941 gagctggtct atcgcctggc ccggccgttc acgctcaaca tcttctcgct gcgcgtcatc
     3001 ccgaacacga cgctggagca gcaaatgatc gccgaggggt tcgacgtcga gcagatcaac
     3061 gagaactaca cggccttgcg gccgacctgg gcgaacgtgc tgctctacat cctcatggtc
     3121 tggaagccgt cacgaaaaac gttcgaccgc tggctgctga aagtgcgcgc ctacaacgag
     3181 ccgcagccgt cgcatccgat cctgattctc ttcgcgcgca tcccctggct gatccagcag
     3241 ggagtgcggc atctcaagca cggcgagttc tcggtgatca ccggctaccc gggctacatc
     3301 ctctggcgca
//
LOCUS       DATLVW010000240         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_53940, whole genome shotgun sequence.
ACCESSION   DATLVW010000240 DATLVW010000000
VERSION     DATLVW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53940"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VNN25_07690"
     CDS             <1..1673
                     /locus_tag="VNN25_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolaconase/LRE-like region family
                     protein"
                     /protein_id="HXH91445.1"
                     /translation="EFQGGAITGNATSIFVALQPGGTYGSGAVGRYNRTTKTRDLLIQ
                     VSAFNNLPRVDVVTGLATAASLLYASDFYGNRVRIFTTDGVWKRDINVTGPGALALDG
                     AGNAWVAQKGEGSIVEFSPTGALLNTIRMPSGSQPSALYFDASSGQLMVGDEGPDMNI
                     KRYKVSGTPALAGTFGIQGGYLDTTTGIKGQVGDKRFTRVTGIGKDTAGNLYVLNNPW
                     GGSWDLGRDGGTDIHSYNSSGRLQWKLQSLNFEGIAAPDPGTDGVYFYGGTNIYTGTA
                     GGTFVANTVDPIDYPSDPRININDRSRDEHFGQLATVGANRILVASGQNPDTYYFFHF
                     NAANGYIAIPDATLPGAAFKTTASVRDGFCIDSKGGVWAGLDKTGYIYHYPLSGFDAS
                     GKPTWGSGIPIRMPESIKPLTRIVYLADSDTMILGRGIVGSTDWTSIGTRIEVYHGWS
                     AGNTTTPNPVINLIHAGAKSIDAAGSYLFVGYWFGSGQALPNIDAFNLATGNLDATLV
                     NTSTGTVDASSAIDSMYGIRAYRRSTGEYVVTKNNVRGSSITVYRWTP"
BASE COUNT          497 a          671 c          689 g          448 t
ORIGIN      
        1 gcgagtttca gggcggcgcg atcacgggca acgcaacgtc gatcttcgtg gcactgcagc
       61 ccggcgggac gtacggaagc ggtgcggtgg ggcgatacaa ccgcaccacc aagactcgcg
      121 atcttttgat tcaggtcagt gcgtttaaca acctgccgcg ggtcgacgtc gtcactggac
      181 tggccacggc ggcttcgctc ctctacgcga gcgacttcta tggcaatcgc gtccggatct
      241 tcactaccga cggtgtctgg aagcgggaca tcaacgtcac gggcccgggc gccctcgcac
      301 tggatggcgc aggcaacgcc tgggtcgcac agaagggtga gggctcgatc gtcgagttca
      361 gtccaaccgg cgcgctgttg aacaccatcc ggatgccgag cgggtcgcag ccatcggctc
      421 tctatttcga tgcgtcatcc gggcagctca tggttgggga cgaaggcccc gacatgaaca
      481 tcaagcgcta caaggtctcg ggcacaccgg cgctggccgg tacattcggc attcagggcg
      541 gttatctcga caccacgacc ggaatcaaag gacaggttgg agacaagcgc tttacccggg
      601 tcactggcat cggcaaggac accgcaggca acctctatgt gctcaacaat ccgtggggcg
      661 gaagctggga cctcggccgc gacggcggca ccgacattca ctcttacaac agttctggtc
      721 gcttgcaatg gaagcttcag tctctcaact ttgaggggat cgccgctccg gacccgggta
      781 ccgacggcgt ctacttctat ggaggcacca acatctacac cggcaccgct gggggaacct
      841 tcgtagcgaa cactgtcgat ccgatcgact atccatccga tccacgcatc aacatcaacg
      901 atcgctcacg ggacgagcac ttcggtcaac ttgccacagt cggcgcaaac cggatcctcg
      961 tggcgtccgg ccagaatccc gatacctact acttcttcca cttcaatgct gcgaatggct
     1021 acatcgcaat accggacgct acgcttcccg gtgcggcgtt caagacgacc gcatcggtca
     1081 gggacggatt ctgcatcgac agcaaggggg gggtgtgggc cggcctggac aagactggtt
     1141 acatctacca ctatccgctg agcggcttcg acgccagcgg caagccaaca tggggatccg
     1201 gtatccccat tcgcatgccc gagagcatca agccgttgac gcgcatcgtc tacctcgcgg
     1261 acagcgacac catgattctc ggacgaggca ttgttgggag tacggactgg acgtcgatag
     1321 gcacgcggat cgaggtatat cacggctgga gcgcgggcaa caccacgaca cccaatccgg
     1381 tgatcaacct catccacgcc ggcgccaagt cgattgacgc ggccggcagc tatctattcg
     1441 tcggctactg gttcggcagc gggcaagctt tgccgaacat cgacgccttc aatctcgcta
     1501 caggcaacct cgacgccacg ctggtcaaca ccagtacagg taccgtcgac gccagcagcg
     1561 ccatcgattc gatgtacggc atcagggcat accgtcgatc gacgggcgag tacgtggtca
     1621 cgaagaacaa cgtcaggggt tccagcatca ccgtttatcg ctggacgcct tgatcgtgcg
     1681 tttcgtcagt gagagaggct tggctatagc cctttacgca ctgtagagca tactcacgag
     1741 tgaacggctg cgttggggaa agtcaactgg ccgctggggg tcgatatcgg tctgtcgccc
     1801 gtagcacgac gcaggttgcc gtgataacca ggcagttatc acggattgaa ggcatcagcg
     1861 catctggccg caccgataat gatgatgggt tgaatagtag tgcagcgttt gcgccacgat
     1921 cgttacttgc tcgtcccccg agttctcgtt aattgggcac aagcgcagct attcacaaga
     1981 gaaaacttat gccaacagcg cggtttggct ccgcgtcgtg cggaaatacc ggaacttggt
     2041 gagagcggtg cgacatgcgc gtcacgacca tctggtcgct gcttgcggcg gcaggccgga
     2101 aaattgattc tgttagtgcg gtggaccgca gacggttgtt gcacggatag atggaattgg
     2161 agataaacat cacaaaaacc agcccttcgc gaggcgcgaa ggatgctgcg caaacaagcc
     2221 ttttgggagt tgttgaatcg gaataatcta ggaatcggtg taatgaatgg gtgacacgtg
     2281 gttatgtgga caactccgcc tttgg
//
LOCUS       DATLVW010000241        13695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_380214, whole genome shotgun sequence.
ACCESSION   DATLVW010000241 DATLVW010000000
VERSION     DATLVW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380214"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VNN25_07695"
     CDS             complement(<1..707)
                     /locus_tag="VNN25_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXH91446.1"
                     /translation="MKQTKRQFIFLALVALLALFAGCKGESPTAPPPLTGTTGTGTGS
                     GATQPPVGATITLVASTNPATTGSISTITATVTQNNAPVPNGTAVEFSTTSANANFTD
                     TTDNPTTLIRTTTAGIAKATVTASSAGPVVVNATVNNVTKSITINFQDAVIPPNPTPT
                     TPTISLVDPSFGLPTGNQTVTITGTNFRPPVRVLFDPGSGQAPKEGFVTSVTPTTITV
                     TTPAFDLGVSQQLIVAIT"
     gene            complement(770..1354)
                     /locus_tag="VNN25_07700"
     CDS             complement(770..1354)
                     /locus_tag="VNN25_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91447.1"
                     /translation="MRMKKIALIATVMLLSVAVLSCNSIAKESSPVLLVVSGTQNLNQ
                     IDLAPGAPGCAASIGTVELTVKLLQNQTDPLTPRNDFNDVKLDRYQVTYQRTDGGKLV
                     PPSFVRSTAQVLTAGGGAQSLSDFVAFETNAFNLAPFAALLPQNGGKDPDTGLGFVKL
                     DIILTVYGSTLAGERVSGTARFPVNFCFNCGGCG"
     gene            complement(1419..4055)
                     /gene="pilQ"
                     /locus_tag="VNN25_07705"
     CDS             complement(1419..4055)
                     /gene="pilQ"
                     /locus_tag="VNN25_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXH91448.1"
                     /translation="MTRAKLILLITGMLVANVTGASAAFWKRNTAAKAPSPAAAAATT
                     PAGMSLTAIDIESAPVPRLILRTTASPVYTSYSPMADVFVIDLTGASKAASLVIPSIL
                     PSSVGSISVEEVTEMGSRLTRVTVHLAQPTTIEAVAEENSIAVNLPAASTAAVAEAAP
                     AAKPDDAPPQVKAVPTVTNAETAPAVKVEIPAPAVAPTVNEPVAEVVKPAIEPSVIAE
                     PLPSTARAKVLRKIETSGAGAAMEVSLATDGDVTYNTFKLQKPARAVIDLDGINDKLA
                     KNIINVSDPVVKRIRVSQFKGAPQPVTRVVLDLDDNATYQVTKTASGLHITFSAASAL
                     ATVATPAAAPPPPPKPKPEQPLVAATPAPPPPAIVVTAKAVPPADIPARVPTIAENEA
                     WKMPEPQKRATSVINAPQDQTPPISTKKKRNTTSPTTAAVVDPTPPAENVFTDPQMAT
                     PPTSQAPSGTLLSAGTGGRTLSGSDKPYTGEPLSLNLKDADIKDVLHTFQQLTGLNIA
                     VDPQVSGAVTVDFTDIPWDQALEIILRQNGLAYVLEGNVMRVGTAARLADEAEANRKL
                     SDQQRLNVPLETVGFKLSYARATDVSTLLREMASPRARIIVDQRTNQLIISEIPLYLQ
                     TMRNLIQSVDVPTRQVVIEARIVETTKTFAQQFGFNWGFRATMDPSLGTGTGLVFPNR
                     IDTVGGPFNFGAGVPALSFTLQNVLGTFNLDLALLAAENEGLVRVISAPRIMTQDNTS
                     AEIQSGFQIPYQTRINLTTTVQYVDATLRLTVTPQITEAGTVIMDIQVQKNEPDTGLN
                     IVGGAGTPLSTRRAQTKLMVRDGGTTVIAGIYQVKENNGQTRMPFLHQIPVLGALFRT
                     HDISSSHDELLIFITPRIVRGS"
     gene            complement(4122..4667)
                     /locus_tag="VNN25_07710"
     CDS             complement(4122..4667)
                     /locus_tag="VNN25_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HXH91449.1"
                     /translation="MSRFVLSLIIVVAAMATLFAQGTAPKKGAPPAPTATTTSATTAT
                     TSPDQTMQQSLQEIVEEPSAPDAYHYDPQGRRDPFQSLIGPAPKLQTGARPAGPPGFL
                     IDEIKIQGVVKTRAGLVAMINGPDNKSYLVKIGDKVLDGEIIRITPNSIVFRQEVNDP
                     TRIERFREVVKEIVSSEVQKK"
     gene            complement(4670..5281)
                     /gene="pilO"
                     /locus_tag="VNN25_07715"
     CDS             complement(4670..5281)
                     /gene="pilO"
                     /locus_tag="VNN25_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXH91450.1"
                     /translation="MAGLSLEGKPWYFGLAIGLVLAIAVVWGVNYAFIGDIDNQIHSA
                     DQQLKDLDAKIEQGRSAQQKLPQFREEVNRLTVELKKLKDILPSTRNTEMIIKKIKQL
                     VDAGNFVLLKLTFPKLAAVQGSDPYAEWPISVSVNGRYHNLAILFNSLGNFPRVINVE
                     QISIGALSPQVDRTITADFVAKTFVYVEPKEPEPADAGKGVKK"
     gene            complement(5290..5976)
                     /locus_tag="VNN25_07720"
     CDS             complement(5290..5976)
                     /locus_tag="VNN25_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXH91451.1"
                     /translation="MIKINLVREGRAVRGTGAATGAASAVSGPTNLNNVFLGALLIAG
                     IVVAGGWYFLENRKLSERQDLLVQKQAEAKRLEAIIKEVENYKKQKESLKTRIDLINQ
                     LKQNQKGPARLMDRISQDLPDLVWLDKMTVIGNTVTLDGRGLNPNAIANFVESIKNDP
                     MFEEPDLGSVTQVSAVPLVYSYTMNFHFTYAPKGAPGTAGAATSTAPGNAAPATGTAA
                     TPPTSTAPKR"
     gene            complement(5973..7031)
                     /gene="pilM"
                     /locus_tag="VNN25_07725"
     CDS             complement(5973..7031)
                     /gene="pilM"
                     /locus_tag="VNN25_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXH91452.1"
                     /translation="MFFSKSKNVVGLDIGSSAVKLIELKEKKGGTFQLVKLGIEHLSP
                     EAIVDGSIMDSSLVVETIGRLNTSKNIKNSNYATSLSGHSVIIKKITLPAMSPEELAE
                     SIQWEAEQYIPFDINDVNLDYVPLTTPGTGDNIDVILVAVKKEKINDYTSVISQTGKV
                     PVLVDVDAFALQNAYEMNYAIDENKVLALVNVGASVTNVNVMSGATSLFWRDITFGGN
                     QYTDAIQRELSLSFEQAEELKKGHQTGDYSIQQVIPILNSVSEDFAGELRKTLDFFTA
                     TSGADRVDEIVLAGGGSGVLNLDAILRDKFGIPVSIMDPFQKVTVDEKLFNPEELAEL
                     GPSMAIAVGLAIRKLGDA"
     gene            7246..7440
                     /gene="rpmB"
                     /locus_tag="VNN25_07730"
     CDS             7246..7440
                     /gene="rpmB"
                     /locus_tag="VNN25_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXH91453.1"
                     /translation="MAKMCEVCGKAPVFGNRVSHAHNVSSRRWLPNLQRVRVMVGAAV
                     KRMNVCTRCIRTGKIAKAAR"
     gene            complement(7575..7967)
                     /locus_tag="VNN25_07735"
     CDS             complement(7575..7967)
                     /locus_tag="VNN25_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91454.1"
                     /translation="MVKKITSEKTKFVRLAGVLSQGAAIDKTSASERVLIPESAIDVD
                     DIVFRAREDIPAVFVQRGDLLIVERRKPDHAATGELVVVLIGDRSFIGHWWTKRGERA
                     LVNDGLDLVTEDKKMRIIGAITAILRWR"
     gene            complement(7972..8451)
                     /locus_tag="VNN25_07740"
     CDS             complement(7972..8451)
                     /locus_tag="VNN25_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91455.1"
                     /translation="MSLDDLVLARVRQFFRDRAPVSLPDAAMIARCDPVWLLTQIEDP
                     ERASAGDAIPWPEVAAIVQRTWTPADLDAAVGTVEGFPTLLRMSLVHWRLPAYLVIAL
                     ERIVVEMRAASPEGDTLTVESYVARLLDLFMDMERVQGLCSDPAFREAFEFPDPEES"
     gene            complement(8698..9288)
                     /locus_tag="VNN25_07745"
     CDS             complement(8698..9288)
                     /locus_tag="VNN25_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91456.1"
                     /translation="MGNLVIVRKLWPNDKRAVVRSGIMQADLLAEFGGLPLAINALLE
                     ALPAASKTDQEPGVELRLGKAYDQSGNPVEAERHFLNAERTMRSSHLNRLESHEILNS
                     VGLFYSRQDNPAEAIQRFHEAQLLQGLDVVAKANLQLSVVKEAIKLPKEQWTKEFATF
                     DSLIAEGRRTTLSPGDAAAIEDLQKHATKVREKTKQ"
     gene            complement(9486..10199)
                     /locus_tag="VNN25_07750"
     CDS             complement(9486..10199)
                     /locus_tag="VNN25_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91457.1"
                     /translation="MIPASMIAVIISGSLPALFALAADNTKTDRVIAQSDIGTVHYKV
                     VECIDPALSAMECHAREQRQLTRRLHEQWITAAAKTYAVVLTDDEQAMVTRELASEKD
                     HFAGAARHFHALALAALEVRRGKDPGLVVPELAKHGVTAKELEWELAHFPTIRDAERA
                     AAIDYFATSEESGRVYLTRRFILQHLQTIVTKRSANEHVSFDVAEEEFWSEIAGAIHM
                     RIIDSTFEMPEKKGILVNP"
     gene            complement(10196..11197)
                     /locus_tag="VNN25_07755"
     CDS             complement(10196..11197)
                     /locus_tag="VNN25_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91458.1"
                     /translation="MRKLYAFWLTVVVFLVPQLASAYCSTYTVILSQTTLFGDGSVSV
                     LITGWDTYEVCDAVGGGTDGGPDGGGSGGGTGSVTSPIPPYITLVNIDTTDPYQPIVT
                     ADVATNDPDDPVNRISLDVNGTTVDSSYSSGTPFARYQLHLPAIIYFPDGTTSLTAKA
                     CSAALVCAQVSANMYRTTPSPNKASVDMFATWLELDEAGTGPLEGPIPVPMSGYFGHS
                     LRQMYTTTLFGGPEVGANSHYLIRDSLVTVSGVLPVPDWSGAVRASGTIGWWNSWGLF
                     TSANPVDCTLPVRCSSKSGGVSGAFGYAPSVTDQVSAVLFEGQPAAVTNTSLSISFP"
     gene            complement(11725..11937)
                     /locus_tag="VNN25_07760"
     CDS             complement(11725..11937)
                     /locus_tag="VNN25_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91459.1"
                     /translation="MQRIRIFGAVVSLFAMLGLTSGAIAAQKKNDNPPARATQSHVQK
                     EMKAPRKGWIAKILDDVADKLSTPPG"
     gene            complement(12035..13372)
                     /locus_tag="VNN25_07765"
     CDS             complement(12035..13372)
                     /locus_tag="VNN25_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91460.1"
                     /translation="MTHPDEAVLSAFSLDAESLADREVVAAHVADCEACQEAIAAFRK
                     FEDALRSPETWMHLTALQSPGDRFAEAEALRRRVQREDEEASRILAPLVNSPLRFRNA
                     KLEEKSRCHTEGMVRMLCAAANERHESRPTFSLELTVTACTIAKALPQTNGAGRRLSM
                     GIALRERANALRYLGKFNDALLALEYAERFFDTSPGADDFDLALVWFIRATVFRSSDR
                     LQEGIVMARDAARVFHEYGERDREISAVMVEAGCLLFLGHTQEAADTFMRVVELARER
                     SNTRVLASALQNGAVALVDLRQLDRAELFYSEALVLFDELGAQTEKARTMWALGSVAV
                     ARGYLEEGALSLDTSRKELARLGMTNDAALATLEWAEVRLALNRPEGVAASCRKIVVV
                     FNSEGMQHHAKEALAALHEGLRAGKATPELVRSVRAYLDQLPANPTQPFVQAS"
     gene            complement(13369..>13695)
                     /locus_tag="VNN25_07770"
     CDS             complement(13369..>13695)
                     /locus_tag="VNN25_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91461.1"
                     /translation="PWLMGAVCNACKHYWRDRKREMPLPEDFEEAIDPTANCSADSWA
                     WRITVAGLLARMGNQCRETLKRYYWADESKESIAATLSLSPGYVLHLLSSCRQRMREL
                     LKRRTS"
BASE COUNT         2568 a         4109 c         4158 g         2860 t
ORIGIN      
        1 gtaatggcga cgatgagctg ctggctcacg ccgaggtcga aggcaggcgt cgtgactgtg
       61 atcgtagtcg gagtgacgct tgtgacgaat ccctccttcg gcgcctgacc gctgcccgga
      121 tcgaaaagga cgcgcaccgg cgggcggaag ttcgtaccgg tgatggtcac ggtctggttc
      181 cctgtcggca gtccaaagct ggggtcaacg aggctgatcg tcggcgtggt cggcgtcggg
      241 ttgggaggaa tgaccgcgtc ctggaagttg atcgtgatgc ttttcgtcac attgttgacg
      301 gtcgcgttca cgacgaccgg accggcgctg cttgccgtga ctgtcgcttt ggctatgcct
      361 gccgtggtgg tccggatcag agtcgtcgga ttgtccgtcg tatcggtgaa attggcgttg
      421 gcgctcgtgg tagagaactc gaccgccgtt ccgttcggca caggtgcatt gttctgcgtg
      481 acggtcgccg tgatcgtcga tatcgagccg gtcgtcgccg ggttcgtcga tgccacgagc
      541 gtgatcgtgg cgccgacggg cggctgtgtc gcgccgctac cggtgcctgt tccggtcgtg
      601 ccggtgaggg gaggaggcgc agtcggtgac tcgcctttgc atcccgcgaa gagcgccaaa
      661 agcgctacaa gcgccaggaa gatgaattgc ctttttgtct gcttcatgtc tacccccgtc
      721 aactcctcat cacacccaca cgaaaccggc cggaaggccg gagtggtaac tagccacagc
      781 caccgcagtt gaagcagaag ttcaccggga acctcgccgt accactcacg cgttcgccgg
      841 ccagcgtcga gccgtaaacc gtgagaatga tgtccaactt cacgaagcca aggccggtgt
      901 ccggatcctt cccgccgttc tgcggaagca gcgcagcaaa aggcgccagg ttgaaggcgt
      961 tcgtctcaaa ggcgacgaag tcactgaggc tctgcgctcc accgcctgca gtcagcacct
     1021 gcgccgtgga gcgcacgaaa ctgggcggca cgagctttcc cccgtccgtc cgctgatacg
     1081 ttacctggta gcggtcgagt ttgacgtcgt tgaagtcatt gcgcggcgtc agagggtccg
     1141 tctgattctg aagaagctta acggtcaact cgaccgttcc aatcgacgcc gcgcaacccg
     1201 gagcccctgg cgccaggtcg atttgattga ggttctgcgt tccggacacg accagcagga
     1261 ccggactgct ctccttggcg atcgaattac aggacagaac cgccacgctg agcagcatga
     1321 ccgttgcgat caatgcgatt ttcttcatcc tcatgcccca ccctccaaaa acacatttcg
     1381 tccacagcat cgaccggttc ctattttatt acgactaatc aggatcccct cacgatccgc
     1441 ggcgtgatga agatcaggag ctcgtcgtgc gaggaactga tgtcatgcgt gcggaacaag
     1501 gctccaagga ccgggatctg atgaaggaac ggcatccgcg tctggccgtt gttttccttc
     1561 acctgataga tacccgcgat gacggtcgtg ccgccgtcgc gtaccatgag tttggtctgg
     1621 gcccggcggg tcgacagcgg cgtgccggcg cctcccacga tattgaggcc ggtgtccggc
     1681 tcattcttct gaacctgaat atccatgatc acggtcccgg cctcggtgat ctggggtgta
     1741 accgtgagac gcagggttgc atcgacgtac tgcacggtcg tggtcaggtt gatgcgggtc
     1801 tggtacggga tctggaaccc gctctggatc tcagccgagg tgttgtcctg ggtcatgatg
     1861 cgcggggcgg agatgacccg gaccagaccc tcgttctccg ctgccagcag cgcgagatcg
     1921 aggttgaacg tgcccagcac gttctgcagc gtgaacgaga gtgcgggaac acctgccccg
     1981 aagttgaatg gaccgccgac cgtgtcgatc cggttgggga aaaccaaacc ggtacctgtg
     2041 ccgagcgacg gatccatggt ggcccggaag ccccagttga agccgaattg ctgcgcgaac
     2101 gttttcgtcg tttcgacgat gcgcgcttcg atcacgacct gccgcgtcgg cacatcgacc
     2161 gactggatga gattccgcat cgtctgcagg taaagaggga tttcactgat gatgagctgg
     2221 ttggtccgct ggtcgacgat gatgcgcgcc cgcggtgatg ccatctcgcg cagaagggtg
     2281 gaaacgtccg ttgcgcgcgc gtaggagagt ttgaagccta ccgtctcgag cggaacgttg
     2341 agccgctgct gatcggaaag cttgcggttc gcttccgcct cgtccgcgag acgtgcggcc
     2401 gtgcccactc gcatgacgtt cccctcgagg acgtacgcga ggccgttctg gcgcaggatg
     2461 atctccagag cctgatccca cggaatgtcg gtgaaatcaa cggttaccgc accggagacc
     2521 tgagggtcga cggcgatatt cagtccagtg agctgctgga aggtgtggag gacgtccttg
     2581 atgtcggcgt ccttgaggtt gagcgaaagc ggctcgcccg tgtacggctt atcgcttccg
     2641 ctgagagtgc ggccgccggt tcctgccgac agcagagtcc cactcggtgc ctggcttgtc
     2701 ggcggcgtgg ccatctgcgg atcggtgaag acgttttcag ccggaggggt cggatcgacg
     2761 accgccgcag tcgttggcga cgtcgtgttc cgtttcttct tcgtcgagat cggcggcgtc
     2821 tgatcctgag gcgcgttgat gaccgaggtg gcccgcttct gaggctcggg catcttccag
     2881 gcctcgttct cggcgatcgt cggaacgcgc gctggaatgt cagcgggcgg taccgctttg
     2941 gcggtcacga cgatggccgg agggggcggg gcgggggtgg ccgcgacgag cggctgctcc
     3001 ggcttcggtt tcggcggcgg gggcggtgcc gccgctggcg ttgctactgt cgcaagcgcc
     3061 gaggcagcgc tgaaggtgat gtgaagaccg ctggcggtct tggtgacctg ataggtagcg
     3121 ttgtcatcga ggtcgaggac gacgcgcgtc accggctgcg gggcgccctt gaactgggaa
     3181 acgcgaatcc gtttcacgac cggatcggag acgttgatga tgttctttgc cagcttgtcg
     3241 ttaatgccgt cgagatcgat gacggcgcgg gcgggcttct gcagcttgaa ggtgttgtag
     3301 gtgacgtcgc catcggtggc gaggctcact tccatcgcag cgccggctcc gctcgtctca
     3361 atcttccgga gcaccttggc ccgggcagtg gacggaagcg gctcggcgat gaccgacggt
     3421 tcgatggccg gtttgacaac ttcggcgacc ggctcgttga ccgtaggtgc aacggcaggc
     3481 gcaggaatct ctactttaac cgctggcgcc gtttcggcgt tcgtgaccgt gggcaccgcc
     3541 ttgacctgcg gcggagcgtc gtcaggcttg gctgcgggag cagcttcggc cacggcggct
     3601 gtgctcgcgg ccggcaaatt gacggcgatg ctgttttctt ccgccaccgc ttcgattgtc
     3661 gtgggctgcg cgagatggac cgtgacacgc gtgagccgcg atcccatctc cgtgacttcc
     3721 tcgacggaga tcgaaccgac ggacgaggga aggatggatg ggatcacgag cgacgccgcc
     3781 ttcgatgcgc cggtaagatc gatcacgaag acatcagcca tcgggctgta ggacgtgtag
     3841 accggactgg cggtcgtacg aaggatcagg cggggcaccg gggccgactc gatatcgatc
     3901 gccgtgagcg acattccggc gggtgtggtg gccgcggcgg cagccggcga gggagccttt
     3961 gcggccgtat tgcgtttcca gaacgctgcg gacgcgccgg taacgttcgc caccagcatg
     4021 cctgtgatca gcaggatcag cttggctctt gtcatacgtc tcaccttccc catctcacag
     4081 ccctccacac acatggcctg cacaacccct gacgcaaacc cctacttctt ctgaacttcg
     4141 gaggagacga tctccttgac gacttcacgg aaccgttcga tccgggtggg gtcattgacc
     4201 tcttgccgga aaacgatcga gttcggtgtg atgcgaatga tctcgccgtc gaggaccttg
     4261 tccccgatct tcacgaggta gctcttgttg tcgggaccat tgatcatggc cacgagacct
     4321 gcccgcgtct tcacgacgcc ctggatcttg atctcatcga tcaggaatcc gggaggtccg
     4381 gccgggcgcg cgccggtctg cagcttcggc gcgggtccga tcagcgactg gaacggatcg
     4441 cggcggccct gcgggtcata gtgataggca tcgggagcgc tgggctcctc gacgatctcc
     4501 tgcagactct gctgcatggt ctgatcggga ctcgtagtcg ctgtcgtcgc agaggtcgtc
     4561 gtcgcggtag gagccggcgg cgcgcccttc ttcggggcag ttccctgggc gaacagcgtc
     4621 gccatcgcag caacgacgat gatcagcgat agaacgaacc ggctcattct catttcttca
     4681 cgcccttccc cgcgtccgca ggctcaggct ctttcggctc gacatagacg aaggtcttgg
     4741 cgacgaaatc ggccgtgatc gtgcggtcca cctgcggcga gagcgcgccg atcgagatct
     4801 gctcgacgtt gatgacgcgc ggaaagttgc cgagtgaatt gaagaggatc gccaggttgt
     4861 gatagcgccc attgacggag accgaaatcg gccactccgc gtagggatcg ctcccctgca
     4921 ccgccgcaag tttcgggaac gtgagcttga gcagcacgaa gttaccggcg tcgacgagtt
     4981 gcttgatctt cttgatgatc atttcggtgt tgcgggtgga cggcagaatg tctttgagct
     5041 tcttcaactc gacggtgaga cggttgacct cttcacggaa ctgcgggagc ttctgctgcg
     5101 cagaccggcc ctgctcgatc ttggcgtcaa ggtccttcaa ctgctgatcg gcgctgtgaa
     5161 tctggttatc gatatcgccg atgaacgcat agttcacccc ccagacgacg gcgatcgcca
     5221 gcaccagccc gattgccagg ccgaagtacc aaggttttcc ttcgagcgaa agccccgcca
     5281 tctaccctcc taccgcttcg gcgccgtcga cgtaggcggc gttgcagccg ttcccgtcgc
     5341 aggtgctgca ttgcccggag cggtacttgt cgccgctccg gccgttccag gggcaccttt
     5401 cggtgcatag gtgaaatgaa agttcatcgt gtacgagtaa accagcggca ccgccgacac
     5461 ctgggtcacg ctgccgagat ccggctcctc gaacatcgga tcgttcttga tgctctcgac
     5521 gaagttggcg atggcattgg gattcaaacc gcggccatcc agcgtcaccg tattgccgat
     5581 gacagtcatc ttgtcgagcc acacgaggtc cgggagatcc tggctgatcc ggtccatcag
     5641 acgggccggc cccttctgat tctgcttgag ctgattgatc agatcgatcc ttgtcttcag
     5701 gctctctttc tgtttcttgt agttttcgac ttccttgatg atcgcctcga ggcgtttggc
     5761 ttcggcctgt ttctggacca ggagatcctg gcgctcggag agcttccgat tctcgaggaa
     5821 ataccatccg ccggcgacca cgatcccggc gatgagcaag gcgccgagga agacgttatt
     5881 gaggttggtc ggtcccgaaa cggcagacgc agcacctgtg gccgctccgg taccccggac
     5941 cgcccgccct tcccgcacta agttaatctt gatcaagcat cccccagctt tcggatggcc
     6001 aggccgaccg cgatggccat actcggacca agctcggcca actcttcggg gttgaatagc
     6061 ttctcgtcga ccgtgacctt ctgaaacggg tccatgatgg aaacagggat gccgaatttg
     6121 tcgcgcagga tggcgtcgag gttcaggacg cccgagccgc cgccggcgag aacgatctca
     6181 tcgacgcggt ctgcaccgga cgtggcggtg aagaaatcga gcgtcttcct caactcgccg
     6241 gcgaaatcct cgctgaccga gttgaggatg gggatgacct gctgaatgct gtaatcgcct
     6301 gtctggtgtc cctttttcag ctcctcggcc tgctcgaacg agaggctgag ctcgcgctgg
     6361 atggcgtccg tgtactgatt gccgccgaag gtgatgtcgc gccagaagag cgaggtggcg
     6421 ccggacatga cgttgacgtt cgtcaccgag gcgccgacgt tgacgagggc cagaaccttg
     6481 ttctcgtcga tcgcatagtt catctcgtag gcgttctgga gggcgaaggc atcgacgtcg
     6541 acgagtaccg gcaccttccc ggtctggctg atgaccgagg tgtaatcgtt gatcttttcc
     6601 ttcttcaccg cgacgaggat gacgtcgatg ttgtccccgg ttccgggcgt ggtcaggggg
     6661 acgtaatcga ggttcacgtc gttgatatca aagggaatgt actgctcggc ttcccactga
     6721 atcgactcag ccagctcctc gggggacatg gccgggagcg tgatcttttt gatgatcacg
     6781 gagtgcccgg agagagaggt ggcgtagttg gaattcttga tgtttttgct ggtgtttagg
     6841 cgcccgatcg tctcgacgac cagcgacgaa tccatgatcg aaccgtcgac gatcgcctca
     6901 ggagaaaggt gctcgattcc cagcttcacg agctggaacg tgccgccctt cttctccttc
     6961 agctcaatca gttttacggc tgatgaaccg atatcgagac ctacgacatt tttgctcttt
     7021 gagaagaaca ccgctcaccc cgtccatctc acacactcac ttctcggcgg ctccagatgt
     7081 gtaaaagtca gtcgaaatta caacgtttaa aggtgagtgt caactctaca ggctttagat
     7141 ttatcagtcg gatacgcgaa gacgagcccg tttccggctc gcaggttgcg ccggaagagg
     7201 gcaaccgata caatcccacc ctttcacgtt atttgaggag tcaagatggc aaagatgtgc
     7261 gaagtctgcg gcaaggcacc cgtgttcggt aaccgggtca gtcatgccca caatgtttcg
     7321 agccgccggt ggctgccgaa tctgcagcga gttcgtgtga tggtgggcgc cgctgtgaag
     7381 cggatgaacg tctgcacacg ctgcatccgt acaggcaaga tcgccaaagc cgcgcggtaa
     7441 aacgcgcggc accttccttc cccttcagcc ccccgttccc tccccgagca atccgttccg
     7501 gatggagttc tgcgcctcgt tttcgtacgg ctggaaccgc gaagagcatc cgaccgcagc
     7561 gcgttgcagt tgcctcagcg ccagcgaaga atcgctgtga tggcgccgat gatccgcatc
     7621 ttcttgtcct cggtgaccag gtccaatccg tcgttgacga gagcacgctc gccgcgtttg
     7681 gtccaccagt ggcctatgaa ggagcggtca ccgatcagga cgacgactaa ctcacccgtg
     7741 gccgcgtggt cgggttttcg gcgctccacg atgaggagat ctccgcgctg aacgaagact
     7801 gctgggatgt cctcgcgcgc gcggaagacg atgtcatcaa cgtcgatcgc gctttccgga
     7861 atgagtaccc gttcgctcgc cgaggtttta tcgattgcag caccttggga caggaccccg
     7921 gcgaggcgca cgaacttcgt cttctcgctc gttattttct ttaccatcac ctcagctctc
     7981 ctcggggtct ggaaattcga atgcttcgcg gaatgccggg tcgctgcaga gtccctgcac
     8041 cctctccatg tccatgaaca ggtcgagcag ccgcgctacg taggactcga ccgtgagcgt
     8101 atcgccttcg ggagaggccg cgcgcatctc gacgacgatg cgctccagag cgatcaccag
     8161 gtaggcgggg agtcgccagt gcacgagcga catgcggagc agcgttggaa acccttcgac
     8221 cgtacccacg gcggcatcga gatcggccgg cgtccatgtc cgctggacga tcgcggcaac
     8281 ctccggccat gggatcgcgt caccggcgga agcgcgctcc ggatcctcga tttgtgtgag
     8341 cagccacacc ggatcacagc gcgcgatcat cgcggcgtca gggaggctta caggcgcacg
     8401 atcgcgaaag aactggcgca ctcgcgcaag gacgaggtcg tcaagagaca cgctcacatt
     8461 gaaggagagc ggaaatgtgg ggacagtcct acctcaggcg aaagcagtcg ccaatagggt
     8521 gcgcgaccga attgggcttt gtcgatcgct tcggatcatt gatgctaggt tttgaagcat
     8581 caaaggcccg ccgagatccc tctactcgac ctctccgtct ccagccgcag cagctcgggc
     8641 ggtccggtgg ccgaaagcag cgttaaagtg actttcagac acgacccaac ggcaccttca
     8701 ctgctttgtc ttctcgcgca ccttcgtggc gtgcttttgc aagtcttcaa ttgcagccgc
     8761 atcgccggga ctgagagttg tacgacgccc ttcagcaatg agggagtcaa acgtagcaaa
     8821 ctccttcgtc cactgttcct tcggcaattt tatggcttcc ttcacgacgg acaactgaag
     8881 gttggcctta gccaccacat ccagaccctg aagcagttgc gcctcatgaa aacgctggat
     8941 cgcctccgcg gggttatctt gacgcgagta aaacagcccc acgctgttga gaatctcatg
     9001 cgactcgagt cgattcagat gtgaggagcg catcgttcgt tcagcgttga gaaagtgtct
     9061 ttcggcttcc accgggttac ctgactggtc gtacgctttt ccgagccgca actccacgcc
     9121 cggctcctgg tccgtcttgc tcgctgccgg caaggcctcc aatagggcgt tgatcgccag
     9181 aggaaggccc ccaaactccg cgaggaggtc agcttgcatg attccgcttc tcaccacagc
     9241 acgtttgtca ttcggccata acttgcggac gatgaccaga ttgcccatcg cctctgcgat
     9301 gagatcctgc ttctgcggcg gcggtgtacg ctccaattgt tgccagagtc gtgaagcctg
     9361 cgcgagctgc gcctcggccg agctcgcagg cgttacaacc gatctcgaag ccgtcgacga
     9421 ggcaatggcc ggagcaacac cacctgacat gacgacgagc gatagaagta tcgataattg
     9481 ttgtctcatg ggtttaccag aattcctttc ttctccggca tctcgaaagt ggagtcgatg
     9541 attcgcatgt gtatcgcccc tgcgatctcg gaccagaact cttcctcggc aacatcgaag
     9601 gagacgtgct cattagcgga acgctttgtg acgattgttt gcaggtgttg caggataaat
     9661 cgacgggtca ggtagacccg gcctgactct tcacttgtgg caaaataatc gatcgctgct
     9721 gccctttcgg catctctgat cgtcggaaag tgggccagtt cccattcaag ctcctttgcc
     9781 gttacaccgt gtttcgccag ctcgggcaca accagcccgg gatccttacc gcgccgaacc
     9841 tccaaggcag ccagcgccag agcatgaaaa tgtcgcgccg caccagcgaa gtgatccttc
     9901 tctgaagcta actcacgggt taccattgct tgctcgtcgt ccgtgagcac gacagcataa
     9961 gtcttcgccg ccgcagtaat ccactgctcg tggaggcgcc ttgtgagttg ccgctgctcg
    10021 cgggcgtggc actccatcgc gctgagagca ggatcaatgc actcgactac tttgtagtgc
    10081 acggtcccaa tgtcactttg agcaatcacc cggtcggttt tggtattatc tgcagcaagc
    10141 gcgaacaagg ccggcagtga gccacttatg atcacagcga tcatgctggc aggaatcatg
    10201 ggaagctaat gctcaacgac gtattggtaa cggcagctgg ctgaccctcg aagagaactg
    10261 cggagacctg atcggtaact gacggggcat agccgaaggc tcctgaaaca cctcctgact
    10321 ttgacgaaca acgcacggga agagtgcagt cgaccggatt cgctgaagtg aacaagcccc
    10381 aggaattcca ccaaccaata gtcccactgg ccctgacagc gccgctccag tcgggcaccg
    10441 gtaggacacc agagacagtt accagcgagt cccggatcag gtagtgcgag tttgcgccga
    10501 cctccgggcc gccgaagagc gttgtcgtat acatttgtcg taagctgtga ccaaaatagc
    10561 cactcatcgg cactggtatg ggaccttcaa gtggcccggt ccctgcctcg tccagttcca
    10621 gccaagttgc gaacatatcc acactcgctt tattaggcga cggagtggtc cgatacatgt
    10681 tcgctgacac ctgcgcgcag actaaagcgg ctgaacacgc cttcgcggtg aggcttgtcg
    10741 taccgtccgg gaagtagatg atcgcgggaa ggtgcagctg atatcgagca aaaggagttc
    10801 cgctgctgta gctggagtct acggtcgttc cgtttacgtc aaggcttatt ctattcaccg
    10861 gatcatccgg atcattcgtg gcgacgtctg ctgtcacgat tggctgatac gggtcagttg
    10921 tgtcaatgtt tacgagagtg atatacggcg ggattgggct agtgacactt ccggtgcctc
    10981 cgccgctgcc cccgccgtcg ggcccgccgt cggtcccgcc gccgacggca tcgcagactt
    11041 cataagtgtc ccagcctgta attaagaccg agacggagcc gtcaccgaag agggtggttt
    11101 gagaaagaat gaccgtgtat gtagagcagt aggcggaggc gagctgcggc acaaggaaga
    11161 cgacgactgt aagccaaaag gcatatagct ttcgcatggt tgagtcctcc atcatgtcgt
    11221 gctctaaggt aaagcctcat gcgcgacacc taaaacgttc tatggccgga gatactagcg
    11281 gcaggcctgt cgcctgtcaa ttgacgtaag aaccgaaagg acgagctgtg ttttgatgct
    11341 ggcgcgcccg caaagggcca tgctcttttc attgcgcgcc gactgcgcct tcggcgaggc
    11401 ccaagcttcg cgaacagcgc caccgcggcc acatacctgc gaatcaccta cattacccct
    11461 cccgctacaa ccgcaggagc gactagcccg gcgggagcga cagcttgttc tctagatcgt
    11521 cgagaaaatc ttcgcgatcc accctttcga cttggcgcgt tgctgcttct gcacgtggct
    11581 tcgtgcatca caagcgcatg tcgccgcagt cctgtcgagc ggcgaacgcg ctcccggcga
    11641 ggcccggcat cgcgaataga gacaccccgg gcacgcacag gcgaatcact tgcatgctcc
    11701 ttcccgacac aaccgaagaa cccactagcc cggcggtgtc gagagcttgt ccgcaacgtc
    11761 gtcgaggatc ttcgcgatcc agcctttacg cggggccttc atttccttct gcacatggct
    11821 ctgcgtggct cgggccggcg gattatcgtt cttcttctgg gctgcgattg caccgctggt
    11881 gaggccgagc atcgcgaaca gagacacgac ggccccaaaa atacgaatcc tttgcataca
    11941 ccctccttgt actggccggc gcagggccgg tcagcggttg ggagggcgcc ctcgggaaga
    12001 ggtgccatca gcgcggcaaa ctatgagatt tttcttacga cgcctgtacg aagggctggg
    12061 tcgggttcgc tggcaactga tcgaggtagg cccggacgct gcggacgagc tcaggtgtcg
    12121 ctttgccggc acggagccct tcgtggagcg ctgcgagggc ttctttcgcg tggtgctgca
    12181 tgccttctga gttgaagacg accacgatct tccggcagga ggcggccacg ccttcgggcc
    12241 ggttgagagc caggcgcacc tctgcccact cgagtgtcgc cagcgccgca tcgtttgtca
    12301 tcccgagtcg ggcaagctcc ttccggctgg tatccagact cagtgctcct tcttcgaggt
    12361 acccgcgcgc tacggccacc gagccgagtg cccacatcgt tcgcgctttt tcagtctggg
    12421 cgcccagctc gtcaaagagt accagcgctt cgctgtagaa gagttccgcg cgatccagtt
    12481 gccgtaggtc aaccagcgct acggcaccat tctgtagcgc gctcgccagc acgcgagtat
    12541 tgctgcgctc acgtgcgagc tcgacaacgc gcatgaaggt gtccgccgct tcttgcgtgt
    12601 gccccaggaa caacaagcag cctgcttcca ccatcactgc cgaaatctcg cgatcgcgtt
    12661 cgccgtactc gtgaaagacc cgagcagcgt cacgcgccat cacgatgcct tcttgaagcc
    12721 gatcgctcga cctgaagacg gtagcgcgga tgaaccaaac gagggccaag tcgaaatcat
    12781 ccgctcccgg cgatgtatcg aagaaccgtt ccgcgtactc cagcgccagt agtgcgtcat
    12841 tgaatttgcc gaggtatcgc agcgcgtttg cacgctcgcg aagggctatg cccatgctca
    12901 accgccgtcc ggcgccattt gtctggggga gggccttcgc gatcgtgcat gccgtcaccg
    12961 tcagttccag actgaacgtc ggacggctct catgccgctc gttggctgcc gcgcacagca
    13021 tgcgcaccat tccttccgta tgacagcgag acttctcctc gagcttcgcg tttctgaacc
    13081 gcagcggcga attcacgagc ggcgcgagaa tgcgcgacgc ttcctcgtcc tccctctgca
    13141 ctcggcgccg cagtgcctca gcctccgcaa accggtcgcc tggagactgc aaggcggtca
    13201 ggtgcatcca agtctccggg cttcgcagcg cgtcttcgaa cttccggaat gccgcgatag
    13261 cttcctggca ggcctcgcag tctgccacat gcgcggccac aacttcacga tcggccagag
    13321 actccgcgtc gaggctgaat gcgctcagga ccgcttcgtc ggggtgagtc atgaagtcct
    13381 cctcttcagc aactcgcgca tgcgctgcct gcatgacgac aacaggtgga gtacgtagcc
    13441 gggcgacaga gagagcgtgg cggcgatcga ctctttcgat tcatccgccc agtagtagcg
    13501 cttcagcgtc tcgcggcatt gatttcccat ccgggccaga agaccggcaa ccgtgatcct
    13561 ccatgcccag ctatccgccg agcagttggc agtgggatcg atggcctcct cgaagtcctc
    13621 gggaaggggc atttcgcgtt ttcgatcgcg ccagtagtgc ttgcacgcat tgcaaactgc
    13681 acccatcagc catgg
//
LOCUS       DATLVW010000242         6661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_380215, whole genome shotgun sequence.
ACCESSION   DATLVW010000242 DATLVW010000000
VERSION     DATLVW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380215"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VNN25_07775"
     CDS             <1..573
                     /locus_tag="VNN25_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91462.1"
                     /translation="SHRGVAESAAISKLLSDAGDAAGLTPEDVHAVKRDLYATIVWHL
                     LADDRLGTAQEAEVLTLREGLGITEEDVPAETNAIEQFRSLRAVIGGMPHAQSPIPLA
                     FQEYCIHQAPLDTGMLIITNKRLIADERKRTEVAMPKVFEVAVDADTNTIAIKSDQKK
                     PLRLSTSNPIFAAGLIDMASALDERPKGFV"
     gene            665..2404
                     /gene="kaiC"
                     /locus_tag="VNN25_07780"
     CDS             665..2404
                     /gene="kaiC"
                     /locus_tag="VNN25_07780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HXH91463.1"
                     /translation="MPPRSTSAAELAKCPTGIAGLDEITGGGLPRGRPTLICGGPGCG
                     KTLFGIEFLVRGVSQGEPGVFVAFEERPEELAQNVTSLGFDLQRLQRQKKLFVEYMHI
                     ERGEIDETGEYDLDALFFRLGDAIDTVGAKRVVLDTLETLFSGFSNDAILRSELRRLF
                     RWLKDRGVTAVITAERGDGVLTRQGLEEYVSDCVILLDHRMSDQVATRRLRVVKYRGT
                     SHGTNEYPFLIDKDGIDVLPITSIGLSHHASTERVASGISDLDEMLAGGIYRGSTALI
                     SGTAGAGKSSIAAHFARASCDRGERCVMFAYEESPQQIARNMKSIGIDLNAHIRSGML
                     QCIAARPTTFGLEMHLATIHKAIRKHSPRMLIVDPITNFFSIASPKEVKGMLMRLVDF
                     LKAEQITAVFTSLTQGGSALEATEAGISSLIDTWLLLRDIESNGERNRTMYVLKSRGT
                     AHSNQMREFVLSKAGIRLIEPYLGVEGVLTGSSRLAQEAKETAATIQKRDETTRKRRA
                     IERRTKEIEAQIAALRLEMAAESDELDRLLASDSDRERKESEDLAIMAASRKSSAKPA
                     ARPRHGKREGFAP"
     gene            2401..2715
                     /locus_tag="VNN25_07785"
     CDS             2401..2715
                     /locus_tag="VNN25_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HXH91464.1"
                     /translation="MRKATKAEAITQEESWNLRLYVVGQTPKSVAAIANLKKFCAEHL
                     ANQYTIEIIDVAANPKIARRDQIVAIPTLVRKLPVPMRKIIGDLSNTERVLVSFDMRP
                     RT"
     gene            2725..3939
                     /locus_tag="VNN25_07790"
     CDS             2725..3939
                     /locus_tag="VNN25_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXH91465.1"
                     /translation="MSDHSSTKTVLPSDVLELRQRALEHEELVRALQGGEIDAVVILD
                     KDNTSISRFQTDEPLYRTMVEALPQSVATVLPDGTIVYANRHLCTMTGTPSETLLGRN
                     LLEVVGEKDKPRFTDMLLRALEAPQEAGISFRWSTGEAPALVSAIRLPIPGSDAVGLI
                     IVDVRDQVARRAAEEASRAKDELLASVSHELRTPLTSIMGWIQLLEIELGGGDANTQP
                     ALQNLKNAVAAETKIVDELLDFAHAEKGSLQLVWQDFDLRQTLRVAASFVEVQAEQKG
                     VTLNIDLPDEALDLRGDPDRLRQVFLNLLTNAVKFTAEGGVSIRCRSDDRSAIVAVSD
                     SGIGIIPEFIPLVFEPFRRSERAQGFPGLGIGMAISRRIVEAHGGTIEVASAGTGQGA
                     TFTVRIPLVAAL"
     gene            complement(4219..6615)
                     /locus_tag="VNN25_07795"
     CDS             complement(4219..6615)
                     /locus_tag="VNN25_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HXH91466.1"
                     /translation="MRIRCLSLVVFVVTVSAALAETGQRLPTGAMLDPVGASHVVGNF
                     PLAMAVAPGGDRVALLLCGWREQGVQIVDRKSGAVLQTLTQPAAFIGLAFSSDGKTLY
                     ASGGNDDSIFVYRWNGREATPDGKIELVEKPKPKPKEKAKEYDGVQYPAGIAVSHDGR
                     FLYATENLGDSLAVIDLAQRRVIQQLHTDRYPYAVVADPHGNVYVSAWGDSTVNVFRA
                     ASDGSLRGTGRIVAGRHPSAMLLRGSRLFVASASTDSISIIDTAERKVVATLKDSPPG
                     GPGEGSTPNALAISRDGGRLFVAEADNNAVAVFDLATKKLLGRVPSEWYPSALATDGD
                     DLLVVSGKGLGSRPNPKRPQLATPKDSHDYTLGQLDGSLLSFSANVSTTKLHAFSERV
                     ARANNWDTTRETPRHPAFKHVIYIIKENRTYDQFFGDMKEGDGDPSLLFFGADVTPNH
                     HALARRFGLFDRFFVNAEVSAQGHNWSTAAYGTDYLEKTTPSAYGTRGRTYDYEGTNR
                     DRVVDDDDDVNAPATGYLWDLAVRKKISLRNYGEFAEAGTDERDSSVTRYRGVRRGLR
                     DTTAPGYPGFNMDISDQTRVDAWLREFRQYGERGSLPSLEIIRLPNDHTRGAAADKPT
                     PRAFVADNDQALGRIVEAVSHSPFWKDTAIFVVEDDAQAGPDHVDSHRSVLLMISAWN
                     RAGVVHRFVNTTDVIATMEELLGLDSLSQFDHFGRPLRAIYAEQPDLTPYDAIKPAID
                     FNEKNPPSEAAKKSAMLDFSREDAADDDTLNRILWSAIKGEAVPYPGATRAPVGTLMR
                     "
BASE COUNT         1318 a         2093 c         2028 g         1222 t
ORIGIN      
        1 tcgcaccgcg gcgtggccga atcagccgcc atctcgaagc ttctcagcga tgccggcgat
       61 gccgccggtt tgacgcccga ggacgttcac gccgtgaagc gcgatctcta cgccacgatc
      121 gtctggcatc tcctcgccga cgaccgcctc ggcaccgcgc aggaggccga ggttctcaca
      181 ttgcgcgaag gattggggat caccgaagag gacgtccccg ccgagacaaa cgcgatcgag
      241 cagttccgca gcctgcgcgc cgtcatcggc ggcatgcccc acgcgcaatc gccgatcccg
      301 ctggcgttcc aggagtactg catccaccag gcgccgctcg acaccggcat gctgatcatc
      361 acgaacaagc ggctcatcgc cgacgagcgc aagcgcaccg aggtggcgat gccgaaggtg
      421 ttcgaagtgg ccgtcgacgc cgacacgaat accatcgcga tcaagtccga tcagaaaaag
      481 ccgctgcgtc tgagcaccag caatccaatc ttcgccgcgg gcctgatcga catggcctcc
      541 gctctggatg agcggccaaa agggtttgtg taagaccgaa acgcctcccg gcgcttcctt
      601 gcgtctggca ggtgcagaca ctatgatccg ttcaggtccc ccgattcatt cctggaacct
      661 ccccatgcca ccaaggtcga cttccgctgc agaactggcg aaatgcccta cagggatcgc
      721 cggtctggat gaaatcaccg gcggtggtct tccgcgcggc agaccgacgc tgatctgcgg
      781 cggtcctgga tgcggcaaga cgctcttcgg gatcgagttc ctcgtgcgag gggtgtccca
      841 gggagaacct ggtgtgttcg tcgccttcga agagcggccc gaagagctag ctcagaatgt
      901 gacatcgctc ggcttcgatc ttcagcggct gcagaggcag aagaagctct tcgtcgaata
      961 catgcacatc gagcgaggcg agatcgatga gacgggcgaa tacgatctcg acgcgctctt
     1021 cttccggctc ggcgatgcca tcgatacggt gggcgccaaa cgtgtcgtgc tcgatacgct
     1081 ggagacactc ttctccggtt tctccaacga tgccattttg cgctccgagc tgcggcgcct
     1141 ctttcgctgg ctgaaggacc ggggagtcac ggcggtcatc acagccgagc gcggcgatgg
     1201 tgttctgacc cgtcagggac tcgaagagta cgtctccgac tgcgtgatcc ttctcgatca
     1261 ccggatgtcc gatcaggtgg ctacgcgcag gttgcgcgtc gtgaagtacc gcggcacgtc
     1321 acacggcacg aatgaatacc ctttcctgat cgacaaagac ggaatcgacg ttcttcccat
     1381 tacgtccatc ggactctccc accacgcctc aaccgaacgc gtcgcaagcg gcatatccga
     1441 tctggacgag atgctcgcag gcggcatcta tcgcggatcg acggcgctca tctccggtac
     1501 cgccggcgcc ggcaagagca gcattgccgc gcatttcgcg cgagcatcct gcgaccgggg
     1561 cgagcgctgc gtgatgttcg cttacgaaga atcaccgcag cagatcgcgc gcaatatgaa
     1621 gtccatcggc atcgatctga atgcgcacat acgcagcggc atgctgcaat gcattgccgc
     1681 ccggccgacc acgttcggtc tggaaatgca tcttgcgacg attcacaagg ccatccgcaa
     1741 gcactcgccg cggatgctca tcgtcgaccc gatcacgaat ttcttcagca tcgccagtcc
     1801 caaggaagtc aagggcatgc tgatgcgtct ggtcgatttc ctcaaagccg aacagatcac
     1861 ggccgtgttc acgagcctga cccagggcgg aagcgctctc gaagcaacgg aggctggcat
     1921 ctcctcactg atcgacacct ggttgttgct gcgcgatatc gaatcgaacg gtgagcgcaa
     1981 ccggaccatg tacgtgctga agagccgtgg caccgcgcac tcgaatcaga tgcgcgaatt
     2041 cgttctctcc aaggctggca tccggctgat cgaaccgtac ctcggcgtcg agggcgtgct
     2101 caccggttcg tcccgcctcg cgcaggaagc gaaagagacc gcggccacga tccagaaacg
     2161 cgacgagacc acccgcaagc ggcgagcgat cgaacgccgt acaaaagaga tcgaggcgca
     2221 gatcgccgcc cttcgtctgg agatggctgc agagagtgat gagctggatc gtctactggc
     2281 cagcgattcc gatcgggaac gcaaggagtc cgaagatctg gcgatcatgg cagcgagcag
     2341 aaagagcagc gccaagccgg ctgcccgtcc gcggcacggg aaacgggagg gtttcgcgcc
     2401 atgaggaaag caacgaaagc agaagcgatt actcaggaag aatcgtggaa tcttcgcctg
     2461 tacgtcgtcg gccagacgcc caagtctgtg gctgccatcg ccaatctgaa aaagttctgc
     2521 gccgaacacc tcgccaatca atacacgatc gagattatcg acgtggccgc gaatccgaag
     2581 attgcgcgcc gcgatcagat cgtggccatc ccgacactgg tgcgaaagct ccccgtcccc
     2641 atgcgcaaga tcatcggcga cctgtcgaat acggaacgag ttctggtgag tttcgatatg
     2701 cgaccgcgaa cctgaggcat cctcgtgagc gatcactctt ccaccaagac ggttctgcct
     2761 tccgatgttc tggagttgag gcagcgggct ctcgagcacg aggaactggt ccgggcactt
     2821 cagggcggcg agatcgatgc ggtcgtcatt ctcgacaagg acaacacgag catctcccgt
     2881 ttccaaacgg acgagccgct ctatcggacc atggtcgaag cactgccgca aagcgtcgct
     2941 acggtgctcc ccgacggcac gatcgtttac gccaaccgcc atctgtgcac gatgaccggc
     3001 acgccctcgg agactctact gggcaggaac cttcttgaag ttgtgggaga gaaggacaag
     3061 ccgcgattca ctgacatgct tctccgtgcc ctggaagcac cacaggaggc cggcatctct
     3121 ttccgctgga gcacgggaga agcgcccgct ctcgtctcgg cgatacgact tcccatcccc
     3181 gggtcagacg ccgtcggcct gatcatcgtc gatgtacgcg atcaggtggc acgccgggcc
     3241 gctgaagagg ccagccgcgc caaggacgaa ctgctcgcat ccgtatctca tgagctgcgg
     3301 acaccgctga cgtcgatcat gggttggatt cagttactgg agattgaact gggcggcggc
     3361 gacgcgaata cgcagcctgc tctgcagaat ctgaagaatg cggtggccgc cgaaacaaag
     3421 atcgtcgacg agcttctcga ctttgcgcat gcggaaaaag gctccctgca gctggtatgg
     3481 caggacttcg acctacgtca gactctgcgc gtcgcggcca gctttgtgga agtgcaggca
     3541 gagcagaagg gtgtgaccct gaacatcgac ttgcccgatg aagcgctcga cctccgcggt
     3601 gacccggaca ggctgcggca ggtgtttctc aacctgctga caaacgccgt gaagttcaca
     3661 gctgaaggcg gagtgagcat ccgttgccgc agtgacgacc gctcggcaat cgtggcagtg
     3721 tcggattccg gtatcggaat catccctgaa ttcataccct tggtcttcga gcctttccgc
     3781 cgcagcgaaa gagcacaggg attcccaggc ctgggaatcg gtatggccat ctcgcgccga
     3841 atcgtcgaag cgcatggcgg gacaatcgaa gtggcgagcg ccggaactgg ccagggggcc
     3901 acattcacgg tacgcattcc gcttgtggca gcgctctgaa gcgcgtcaac gcacaagagc
     3961 ttcgtgctgt tcgttttgca aaacacccct catccgcctt cggcaccttc tcccctcgaa
     4021 aagcacgagg ggagaaggcg ctcgattcat gagcatggcg tgaggggaac gaccaagctt
     4081 cgcgactacc tctccccatt cacaactacc tctcccacga gcgccttctc ccccacgctt
     4141 cttgtggggg agaaggtgcc gaaggcggat gagggggcgt ttgatgtcgc gccaaacgtt
     4201 gccaaaatac gagcgccttc accgcatcaa tgttccaacc ggcgccctcg tcgcacccgg
     4261 atacggcact gcctccccct tgatcgcgct ccacagaata cgattcagcg tgtcgtcgtc
     4321 cgcggcgtcc tcacgggaga aatcgagcat cgcggacttc ttcgccgctt cgctcggagg
     4381 attcttctcg ttgaagtcga tcgctggctt gatcgcgtcg tacggcgtca ggtcgggctg
     4441 ctcggcgtag atcgcacgga gcggacggcc gaagtgatcg aactgggaga gggagtcgag
     4501 gccgaggagc tcttccatcg tggcgatgac gtcggtcgta ttcacgaagc ggtgtacgac
     4561 gccggcgcgg ttccaggcgg agatcatcaa tagtaccgac cggtgcgaat cgacatggtc
     4621 cggtccggcc tgcgcgtcat cctccacgac gaagatcgcg gtgtctttcc agaacggcga
     4681 atgcgagacc gcttcgacga tgcggcccag cgcctggtcg ttgtcggcga cgaatgctcg
     4741 cggcgtcggc ttgtctgccg ctgcgcctcg ggtgtgatca ttcggcaggc ggatgatctc
     4801 cagcgacgga agcgaaccgc gctccccgta ttgccggaac tcccggagcc acgcatcgac
     4861 gcgcgtctga tcggagatgt ccatgttgaa tccgggatag ccgggagcgg tggtatcccg
     4921 cagtccgcgg cgaacgcctc tgtatcgcgt gacggagctg tcgcgctcgt cggtgcccgc
     4981 ttcggcgaac tcaccgtagt tgcgcaacga gatcttcttg cgcactgcca gatcccaaag
     5041 gtagccggtg gccggcgcat tgacgtcatc atcgtcatcg accacccggt cgcggttcgt
     5101 tccttcgtaa tcgtaggtgc ggccgcgcgt gccgtacgcc gagggcgtgg tcttctcgag
     5161 gtagtcggtg ccgtacgccg ccgtcgacca gttgtggccc tgcgcgctca cctcggcatt
     5221 cacgaagaaa cggtcgaaca gaccgaaacg ccgcgccagc gcgtggtggt tcggtgtgac
     5281 gtccgcgccg aaaaagagca gcgacggatc gccatcccct tctttcatat cgccgaagaa
     5341 ctgatcgtac gtgcggttct ctttgatgat gtagatgacg tgtttgaacg ccggatggcg
     5401 cggagtctcg cgcgtcgtat cccagttgtt cgcgcgcgcc acacgctccg agaaggcgtg
     5461 cagcttcgtg gtggaaacgt tcgctgagaa cgagagcagt gagccgtcga gctggccgag
     5521 ggtgtagtcg tgcgaatcct tcggcgtcgc cagttgcggg cgtttcggat tgggacgtga
     5581 accgagccct ttgccgctga ctacgagaag gtcatcgccg tccgtagcca gcgccgacgg
     5641 ataccactcc gagggcacgc gtccgagcag cttcttcgtg gccaggtcga agaccgccac
     5701 cgcgttgttg tccgcctcgg ctacgaagag ccggccacca tcgcgcgaga tcgccagcgc
     5761 gttcggcgtg ctcccctcgc ccggaccgcc cggcggcgag tccttcagcg tggcgacgac
     5821 tttgcgttcg gccgtgtcga tgatgctgat cgagtccgtg ctcgccgagg cgacaaagag
     5881 acgtgagccg cgaaggagca tcgccgatgg atgccggccg gcgacgatgc gcccggtgcc
     5941 gcgcaagctg ccgtcactgg cagcgcgaaa aacattgacc gtgctatccc cccacgccga
     6001 tacgtagacg ttgccgtgtg gatcggcgac taccgcatac ggatagcgat cggtgtgcaa
     6061 ctgctggatg accctccgtt gtgcaagatc gatcaccgcg agcgaatcgc cgaggttttc
     6121 tgtggcgtag agaaaacgac cgtcgtgcga gaccgcgatc ccggccggat actgaacccc
     6181 gtcgtactcc ttcgccttct ccttcggttt cggtttcggc ttctcgacca gctcgatctt
     6241 gccgtcgggc gtcgcttcac gcccgttcca gcgatagacg aaaatcgagt cgtcgttgcc
     6301 gccgctggcg tagagagtct ttccgtcgga agagaaagcc agaccgatga acgcggccgg
     6361 ctgcgtcagc gtctgcagca ccgcccccga cttgcgatcg acgatctgca cgccttgctc
     6421 gcgccatccg cagaggagca gcgccacgcg gtccccaccg ggcgcgacgg ccatcgcaag
     6481 cggaaaattg ccgacgacat gagacgcacc aacaggatcg agcatcgctc ccgtcggcag
     6541 tctctgccct gtctcggcaa gggcagcgct gacggtcacg acaaatacaa ccaacgatag
     6601 gcaacgaata cgcatgcagg cgatgatatc ggtatgaaca ctccgatgcc cgcacttttc
     6661 a
//
LOCUS       DATLVW010000243         3835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_216574, whole genome shotgun sequence.
ACCESSION   DATLVW010000243 DATLVW010000000
VERSION     DATLVW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216574"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..350
                     /locus_tag="VNN25_07800"
     CDS             216..350
                     /locus_tag="VNN25_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91467.1"
                     /translation="MTFLEQLFRINLDGGNGLFELLLLLVPLTGILMLRVRHLRRRRA
                     "
     gene            442..1815
                     /locus_tag="VNN25_07805"
     CDS             442..1815
                     /locus_tag="VNN25_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXH91468.1"
                     /translation="MARYRVTVAGKNYDEMADLVRKYKLNIAGHTGKNVGGGRYVVDA
                     FADSDQIATLKSAGYGVDVHEDVQEAGTMRQAEVRSPAVAGAAASSSVATSNHYLNVD
                     DVETSLAAAAAAPNTAFTKLIALPNKTWENRDCHALKIGNGNQANRPGIFLLGGIHAR
                     EWGSSDILINFVEQLCDAFRTNSGITLGGASFTAAQIQQIVNQKDIYVFPQANPDGRN
                     HSMTAAPDWRKNRRPAPAGSHNPDCVGVDLNRNYDFLWNFTTHFAPNAPVQNSTNPCD
                     HDIYVGPAPMSEPETKNVVWLFENFSNVRFFIDVHSYSEDILYSWGDDDDQTKKPQMN
                     FQNPAFDGKRGVANDTGYKEFIPAADKTAAVNLANQMRTAIQAVRGRNYTVMQSMSLY
                     PTAGTSDDYAYSRHIVDSSKPKVLSYTIEWGSPNNPTPFHPPYAEMKQIIQEITAALL
                     QFCLRAA"
     gene            complement(2225..>3835)
                     /locus_tag="VNN25_07810"
     CDS             complement(2225..>3835)
                     /locus_tag="VNN25_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXH91469.1"
                     /translation="RAILEVESLARQALTGNQQSEQTLLIASEAVRLARASHLPVEHL
                     AEYALRQTIARMGGKPIGAQPLLDSHTTTPRRDPFFVSAGSNSGLVSRIRGGALERIG
                     NVPVSVSAISADGRSIASCASEVVVQRDWRRQTAFHVGNDAQGRCALSGDGRWLAFGD
                     FAYDLKADHMAGSGVRLAELWNPDFQFSSDGKWLIASSGQDLWLWSVGASGPERPPRL
                     QIRSDNWAGRIAPPPSSPDNPGVTAASPPEPLPDPSLVPRAVKTGDVYVQQTIDEAVT
                     WSGDGRWLAVASNSAAIVLDLHDPGLRTHVLDMKAESPHRLPAMLFTASSKWLIVNGQ
                     TPRAYRAPSFRRGIPIGMLPVTAMAASEDGHWLATATEDVRVWDLRASTPFASPRYLL
                     RGAVSRKETIESLGGAVRSLVFSPNGRWLISASASAELWDLQQPDPTLAPIALTLPDL
                     TKLRFLPNPDSWFLRYWFTPDSRHLILYGAEGLNEDLPTHYALYPLEGDDLLQFASAN
                     LHRGFTDAERKWLFPEEPIASAVPAKPQ"
BASE COUNT          762 a         1225 c         1153 g          695 t
ORIGIN      
        1 catgtctggg cgacgctgac cgccctcaac cggctggtca gtgagccggg attggtggct
       61 ggacgcgcgg cgattgccat cgcctccggc tgcgtggcgt tctgggcctg gcggtcgtct
      121 cgttcgtccg ggcgcgcggc gtgaagggct tgaactttgc ggcgggggta gggaaaagag
      181 gcgaaatgtc cctcctataa gaagaggaga ttcctatgac cttccttgaa cagctcttcc
      241 gaatcaacct ggacggcggc aacggtctct tcgagctcct tctgctgctc gtccctctga
      301 ccgggatcct gatgctgcgc gtgagacacc ttcgccggcg ccgcgcctga accgtgatcc
      361 cgagactcat ccgcagatgt cgcagatgtt gatctgcgtc tcctctgcgc catctgcgga
      421 tcgcctccac aggagtgacc aatggctcgc tatcgcgtga ccgtagcagg gaagaactac
      481 gatgagatgg ccgaccttgt tcgcaagtac aagctgaaca tcgcaggcca caccggcaag
      541 aacgtgggcg gcggacgtta cgtcgtcgac gcgtttgccg attccgatca gatcgcgacg
      601 ttgaagtccg ccgggtacgg cgtcgacgtc cacgaagacg tgcaggaagc ggggacgatg
      661 cgtcaggccg aggtgcggtc acccgcggtg gcgggagcgg ctgcgtccag cagcgtcgcg
      721 acatcgaatc actatctcaa tgtcgacgac gtcgagacct cgcttgcggc tgccgcagcc
      781 gctccgaaca cggccttcac gaagctcatc gcgctgccga acaagacgtg ggaaaaccgg
      841 gactgccatg ccctcaagat cggcaacggc aatcaggcca accggccggg catcttcctg
      901 ctgggcggga ttcacgcgcg ggaatggggc agctcggaca ttctcatcaa cttcgtcgag
      961 cagctctgcg acgccttccg aaccaattcc ggcatcaccc tcggcggcgc gagtttcacg
     1021 gccgcgcaga tccagcagat cgtcaatcag aaggatattt atgtcttccc gcaggccaat
     1081 ccggacggcc gcaatcacag catgaccgcc gctccggatt ggcgaaagaa ccgccgcccc
     1141 gcccccgccg gcagccacaa ccccgattgc gtgggcgtcg atctgaaccg caactacgac
     1201 ttcctttgga acttcaccac gcacttcgct cccaacgccc cggtacagaa ctcgaccaat
     1261 ccctgcgatc acgacatcta tgtcggcccg gccccgatgt cggagccgga gacgaagaat
     1321 gtggtgtggc tgttcgaaaa cttctcgaac gtccgcttct tcatcgacgt ccacagctat
     1381 tcggaagaca tcctctatag ctggggagac gacgacgacc agacgaagaa gccgcagatg
     1441 aactttcaga acccggcctt cgacggcaag cgcggcgtgg cgaacgatac cggctacaag
     1501 gagttcatac ccgctgcgga caagaccgcg gccgtcaatc tcgccaatca gatgcgcacc
     1561 gccatccagg ccgtgcgcgg ccgcaactac acggtcatgc agtcgatgag tctctatccc
     1621 acggccggaa cgtccgacga ttacgcctac agtcgccaca tcgtcgattc gtccaagccg
     1681 aaggtcctct cgtacacgat cgagtggggc agcccgaaca acccgacgcc gttccacccg
     1741 ccgtacgcag agatgaaaca gatcatccag gagatcaccg cggcgctgct gcagttttgt
     1801 ctgcgtgcag cgtaggtctt cgcgcggcgg aaaaaaagac tgcaccacag agacgctgag
     1861 gacacagagt tgacggccca ctctgcgacc tctgtgtctc tgtggttcaa agggttttcc
     1921 gaccctcgga ccacaacgtc ggcgcctcgc acgcgcgtga cgatcgcgtt tgtgggataa
     1981 cagcttcgac gcaaacatct gccgcctcga cccgccggca aagggctgga gccagaacct
     2041 gtatctgagt tggttcgcga cgaatgttgg agggctacct tctaatcatc gagcgacgac
     2101 ggtgttcggg agctcctgac agaaaggggc ctccacgtcg tgatgcagca agactgggcg
     2161 ccatccccga ttcgtgaaag cctcagcttc ggtttccgtc tggcaatatc tgcgcgtgac
     2221 gcggtcactg cggttttgcc ggcaccgcgg acgcgatcgg ttcctccgga aacagccact
     2281 ttcgttcggc gtcggtgaag ccgcggtgca gattcgcgga cgcaaactgc agaaggtcgt
     2341 cgccttccag cggatagagc gcgtagtgcg tcggcaggtc ctcgttgaga ccttccgcgc
     2401 cgtacaaaat caagtgccgc gaatcagggg tgaaccagta ccgcagaaac cacgaatcgg
     2461 gattggggag gaatcgcaac tttgtcagat ccggaagtgt caacgcgatc ggtgccaggg
     2521 tcggatccgg ctgttgaaga tcccatagct cggcagatgc acttgcactg atgagccatc
     2581 gaccattcgg gctgaatacg agcgatcgaa cggcgccacc aagcgactcg atcgtctctt
     2641 ttcgcgatac tgcgccgcgc agaaggtaac gcggactcgc gaacggcgtc gaagctctga
     2701 gatcccatac ccgcacgtct tcggttgccg tcgccagcca gtgtccgtcc tcgctcgccg
     2761 ccatcgctgt gaccggtagc atcccgatcg gtattccccg gcggaacgaa ggtgcgcgat
     2821 atgcgcgcgg tgtctggccg ttgacgatga gccacttgct ggacgcggtg aacagcattg
     2881 ccggaagcct gtgcggactc tccgccttca tgtccagcac gtgcgtgcgc agtcctggat
     2941 catgaagatc cagcacgatc gccgccgaat tcgatgctac tgcgagccat cggccatcgc
     3001 ccgaccacgt caccgcctca tcgatggtct gctgaacgta tacgtcgccc gtctttacgg
     3061 cgcgcggcac cagcgatgga tcgggcagcg gctcaggcgg cgaggcagca gtcacgccgg
     3121 ggttgtctgg gctcgacggc ggcggagcga tgcggccagc ccaattgtcg gatcgaatct
     3181 gcagccgcgg cggacgttcg ggtcccgagg ccccaacgga ccataaccac agatcctgac
     3241 cactcgacgc gatgagccat ttaccgtcgc tgctaaactg gaagtctgga ttccagagct
     3301 ccgcaagccg gacgcccgag cccgccatgt ggtctgcctt gagatcgtag gcgaagtcgc
     3361 cgaaggcgag ccatcgtccg tcgccactga gagcgcacct tccttgtgca tcgttcccga
     3421 catggaatgc ggtctgacgc cgccaatcac gctggaccac cacttcgctc gcgcatgaag
     3481 caatcgagcg tccgtccgcg ctgatcgcgg atacggatac cggaacatta ccgattcgtt
     3541 ccagggctcc ccctcggatt cgcgaaacga gccccgagtt actccccgcc gagacgaaga
     3601 agggatcgcg tcggggcgtg gtcgtgtgcg agtccagaag cggctgggca ccaatcggct
     3661 ttccgcccat tcgcgcaatc gtctgacgga gcgcatattc cgcaagatgc tcgacgggta
     3721 gatgcgaagc tcgcgcaagc cgcaccgcct cagaggcgat caggagcgtc tgctctgatt
     3781 gttggttgcc cgtaagagcc tgacgtgcca gcgactcgac ttccaggatc gcgcg
//
LOCUS       DATLVW010000244         5038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_380240, whole genome shotgun sequence.
ACCESSION   DATLVW010000244 DATLVW010000000
VERSION     DATLVW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380240"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..327)
                     /locus_tag="VNN25_07815"
     CDS             complement(70..327)
                     /locus_tag="VNN25_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH91470.1"
                     /translation="MPIGRKYNADADRFGAILRQLREQRGWTRQKLAQRAGLATTYIG
                     VLEAGGNVPTISTALELIEVLGGDIGDIMRRLAAARVAPPG"
     gene            593..2488
                     /gene="parE"
                     /locus_tag="VNN25_07820"
     CDS             593..2488
                     /gene="parE"
                     /locus_tag="VNN25_07820"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HXH91471.1"
                     /translation="MTTTYDSSAIEVLTGLEPVRKRPGMYTTTERPNHLAQEVIDNSA
                     DEAIAGHCDEIVVTLHDDNSVTVADNGRGMPVDRHAKEGVTGVEVILTRLHAGAKFSD
                     KSYRFSGGLHGVGVSVVNALSKKLEVWVRRDGNEYLMVFGDGKKKQELKIVGQVGKRN
                     TGTTIRFWPDEKFFDTPKINVPRLKHVMRAKAVLAPGLRLRFINENDATDNEEWYYEN
                     GLPAYLMSELAGLTTIPEMPYVGTFKGSNEEVDWAVVWVPDDNELVTESYVNLIPTAQ
                     GGTHVNGFRTGLTEAIREFCELRNLLPRGVKLAPEDVWSRCSYILSLRMTEPQFTGQT
                     KDRLSSREASAFVSGVAQDAFSIWLNNNVADAERIAQVAISSAMTRLRQGKKVERKKL
                     TSGPALPGKLADCTSTDLNLTELFLVEGDSAGGSAKQARDREYQAIMPLRGKILNTWE
                     VDPNEVLSSQEVHDIAVALGVDPGSDDVKGLRYGKVCILADADSDGAHIATLLTALFV
                     RHFRKLVTDGRIHVAMPPLYRIDVGKNVFYALDDHERDGILQRLEAEGIRGKVNIQRF
                     KGLGEMNPLQLRETTMNRDTRRLVQLVLVAEDGADATIDMLLAKNRAGDRKQWLTEEG
                     DKAEILE"
     gene            2568..3788
                     /locus_tag="VNN25_07825"
     CDS             2568..3788
                     /locus_tag="VNN25_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HXH91472.1"
                     /translation="MAIASVETIRKHFPALDRIHNGQPVAYFDAPGGTQVPRVVAEAM
                     TEYLFHHNANTHWLYPTSEETDAIIERSREAAADLLNADRAEIIFGANMTTLTFHLAR
                     ALGRMWQAGDEVIVTELDHHANVAPWRALEVDRGIVVRTARMIPETAQLDYEHMASLV
                     SPRTKLIAVGAASNAVGTINDLGRVRAIARDAGALFFVDAVHLVPHELIDVRAIGCDF
                     LACSSYKFYGPHAGILFARRELLDVLPFPKLDPAPDWSPERAETGTQNHEAIAGIGAT
                     VDFLVSLGGEEGSRRERLARTFAVLHSRGDVQLRMLWNGLTSLPNVRVYGPPPGQPRT
                     PTVSFTIEGIHPDDVARHLAANGLFASNGDFYALTCIERLGCPDGVVRIGCAAYTTDD
                     EVVRLLEELSFMVG"
     gene            complement(4015..>5038)
                     /locus_tag="VNN25_07830"
     CDS             complement(4015..>5038)
                     /locus_tag="VNN25_07830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXH91473.1"
                     /translation="TYFALSGGGWIAWGVVFPIATLALRLLLPQRIALNVMMMAIVLA
                     ALFLAPEGRMKRNTTGILVALSLINAGSLAVGALGSRNARRTAIEEGVQRRSEVREQL
                     RIRDELRFAREVQISMLPEAPPKLDWADIAGVSIPATEVGGDYYDYYIVDGRLAVICG
                     DVAGHGLASGITLSALRSGFTLLRDALLDPAHVLERLQEVVTQSSRRRMMVTISVLLL
                     DSETQRATIASAGHPPLLQIRAGAPQFVELHSLPLGARLGEHVPQEVVPFASGDIFVL
                     HSDGIYETVNARGETYGLERLGRIAAACDGNAEQIRDAVLRDVETFRGTEAQQDDVTL
                     VVVKIG"
BASE COUNT          992 a         1619 c         1550 g          877 t
ORIGIN      
        1 ggcgcgaatg tgctccacct cgccgttcgt tacaccatgg tagacgtaga tacacgcgac
       61 ggccgggaat cagcccggcg gagccacgcg cgcagcagcg agacgacgca taatgtcgcc
      121 gatgtcgccg ccgaggactt cgatcagctc gagggccgta gagatcgtcg ggacattgcc
      181 acccgcctcc agaacgccga tatacgtcgt cgccaaaccg gcgcgttggg ccagcttctg
      241 gcgcgtccag ccgcgttgct cgcggagctg gcgaaggatg gcgccgaaac ggtcggcgtc
      301 cgcgttgtac ttcctcccga tcggcatggt tggagtggtc ggcaaatatc gtaacgcatt
      361 agtgaggagt ccgggcgtct cggaacgtgc gttagaatcg acgccgtctc gcgcgggcga
      421 gtgcgtgatg cgcggagcga ggcgaaacac caaggagaac gatgaaccac agagacacag
      481 agggcacaga gttgaagcaa tgactctgtg atctctgtgt ctctgtggtg aatggttttg
      541 gtgatcaacg ctccaggtcg acctgaggca aaaccattcc atttcgacac ccatgaccac
      601 tacctacgac agttctgcca tcgaagtcct caccggactc gagccggtgc gcaagcggcc
      661 cggcatgtac accaccaccg agcggcccaa ccatctcgcg caggaagtca tcgacaactc
      721 cgccgacgaa gccatcgccg gacactgcga cgagatcgtc gtcacgctgc atgacgacaa
      781 ctcggtcacc gtcgccgaca acggccgcgg catgcccgtc gatcgccatg caaaggaagg
      841 cgtgaccggc gtcgaagtca ttctcacgcg tctccacgcc ggcgcgaagt tctccgacaa
      901 gagttatcga ttctccggcg gtctgcacgg cgtcggcgtc tccgtcgtca atgcgttgtc
      961 gaagaagctc gaagtgtggg tgcgtcgcga cggcaacgaa tacctgatgg tcttcggcga
     1021 cggcaagaag aagcaggagt tgaagatcgt cggacaggtc ggcaaacgca atacgggcac
     1081 gacgatccgc ttctggcccg acgagaaatt cttcgacacg ccgaagatca atgtcccgcg
     1141 cctcaagcac gtcatgcgcg ctaaagccgt tctcgcgccg ggcctgcgcc ttcgcttcat
     1201 caacgagaac gatgcgaccg acaacgagga gtggtactac gagaacggct tgccggctta
     1261 tctgatgtcg gagctggccg gtctcaccac gattccggag atgccgtacg tcggcacgtt
     1321 caaaggatcg aacgaggaag tcgattgggc tgtcgtctgg gtgcccgacg ataacgagct
     1381 ggtcacggag tcgtacgtga acctcattcc tacggcgcaa ggcggcacgc acgtcaacgg
     1441 cttccgcacg ggactcaccg aggccattcg cgaattttgc gagctgcgca atctcctgcc
     1501 gcgcggcgtg aagctggcgc cggaagatgt ctggtcgcgc tgcagctaca tcctttcgct
     1561 gcgcatgacg gaaccacagt tcaccggcca gacgaaggat cgattgtcat cgcgcgaagc
     1621 atcggcgttc gtctccggcg tcgcgcagga tgcgttctcg atctggctga acaacaacgt
     1681 cgccgacgcc gagcgcatcg cccaggtcgc aatctcgtcg gccatgacgc gtctgcgcca
     1741 gggaaagaaa gtcgagcgca agaaactgac gagcggtccc gcgcttcccg gcaaactcgc
     1801 cgattgcaca tcgaccgatc tcaatctcac cgagctcttc ctcgtcgaag gtgactccgc
     1861 cggcggatcc gccaagcagg cgcgcgatcg cgagtaccag gccatcatgc cgttgcgcgg
     1921 caagatcctg aacacctggg aagtcgatcc gaacgaggtc ctctcctcgc aggaagtgca
     1981 cgacatcgcc gtcgccctcg gcgtcgatcc cggttccgac gacgtgaagg gcttgcgcta
     2041 cgggaaggtc tgcatcctcg ccgacgctga ctccgacggt gcgcacatcg cgacgctcct
     2101 gaccgcgctc ttcgtccgcc acttccgcaa gctcgtcacc gacggccgca tccacgtggc
     2161 catgccgccg ctgtaccgca tcgacgtcgg caagaacgtc ttctacgcgc tcgacgacca
     2221 cgagcgcgac ggcatcctgc aacgcctcga ggccgaaggg atccgcggca aggtcaacat
     2281 ccagcgtttc aaaggcctcg gcgagatgaa tccgctccag ctccgcgaga cgacgatgaa
     2341 ccgcgacacc cgccgcctcg tccaactcgt cctggtagcc gaggatggcg cggatgcaac
     2401 gatcgacatg ctgctggcaa aaaaccgagc cggcgaccgc aaacagtggc tgaccgaaga
     2461 aggggacaag gcggagattt tggagtgagg ttgatccgca gatgacgcag atgtacacag
     2521 attctcatct gcgttcatct gcgtcatctg cggataaaga ttctcttatg gcaatcgcat
     2581 ccgtcgaaac catccgcaaa cacttccccg cgctcgatcg cattcacaac ggccaacccg
     2641 tcgcctattt cgatgcgcct ggtggcacgc aggtgccgcg cgttgtggcc gaggcgatga
     2701 cggagtatct ctttcaccac aacgccaaca cgcactggct ctatccgacc agcgaggaga
     2761 ccgacgcgat catcgaacgt tcgcgcgagg cggccgccga tctcctgaac gccgatcgcg
     2821 ccgagatcat cttcggcgcg aacatgacca cgctcacgtt tcacctcgcg cgcgcgctcg
     2881 ggcgcatgtg gcaggcaggc gatgaagtca ttgtcaccga gctcgatcac cacgccaatg
     2941 tcgcgccgtg gcgggcgctg gaggttgatc gcggaatcgt cgtgcgcacg gcgcggatga
     3001 ttccggagac agcgcaactc gattacgagc acatggcctc gctcgtctcg ccgcgaacga
     3061 aactgatcgc ggtcggcgcg gcatcgaatg ccgtcggtac gatcaacgat ctcgggcgcg
     3121 tccgcgcaat cgcacgcgac gccggagcac tcttctttgt cgatgctgtg catctcgtgc
     3181 cgcacgagct gatcgacgtg cgcgccatcg gctgcgattt tcttgcttgc tcgtcgtaca
     3241 agttctacgg cccgcacgcg ggcattctct tcgcgcgtcg cgagctgctc gacgtgctgc
     3301 cgttcccgaa gctggatccg gcgccggact ggtcgccgga acgggcggag acaggaacgc
     3361 agaatcacga agcgatcgca ggcatcggcg cgacggtcga tttcctggtc tcgctcggcg
     3421 gtgaagaagg atcacgccgc gagcgtctcg cccgcacgtt cgctgtgttg cattctcgcg
     3481 gcgacgtgca actgcgcatg ctgtggaacg gcctgacatc gctgccgaac gtgcgcgtct
     3541 atggtccgcc gccgggacag ccgcgcacgc cgacggtgtc gttcacgatc gaagggatcc
     3601 atcctgatga cgtcgcgcgc cacctcgcgg ccaacggtct cttcgccagc aacggcgact
     3661 tctacgcgct cacctgcatc gagcgcctcg gatgtccgga cggcgtcgtt cgcatcggtt
     3721 gcgcggcgta cacgacggat gatgaagtcg ttcgattgct ggaagagttg tcgttcatgg
     3781 ttggctgaag cacagacaag agtgtctgtg ccacactgat ccggccttcg tggcacaacc
     3841 aaggagcacc tgagcctcga agccgaaagt gatgtggaac agacactctt gtctgtgctc
     3901 ctgaataact acccgatcct gaccacgacc agcgtctggc acaaccaagg agcacctgag
     3961 cctcgaagcc gaaagtgatg tggcacagac actcttgtct gtgctcccga gtaactaccc
     4021 gatcttcacc acgaccagcg tgacatcgtc ctgctgcgct tccgttccgc ggaacgtctc
     4081 gacgtcgcgc aacaccgcat cgcggatctg ttccgcgttg ccatcgcagg cggcagcgat
     4141 gcggccgagc cgttccagcc cgtacgtttc gccgcgtgcg ttgacggtct cgtagatacc
     4201 gtcgctgtgg aggacgaaga tgtcgccgga agcgaagggg acgacttcct gcggcacgtg
     4261 ctcgccgagc cgcgcgccga gcggtagcga gtgaagctcg acgaactgcg gagctcccgc
     4321 gcggatttga agaaggggag gatgccctgc cgaagcgatc gtggcgcgtt gcgtttcgga
     4381 atcgagcagc agcaccgaga tcgtcaccat catcctgcgg cggctcgact gcgtgacgac
     4441 ctcctgcaag cgctcgagta cgtgcgcagg atcgagcaac gcatcgcgca gcagggtgaa
     4501 gccgctgcga agcgcggaga gcgtgatgcc gctggcgagg ccatgtccgg cgacgtcgcc
     4561 gcagatcaca gcgaggcgtc cgtcgacgat gtagtagtcg tagtagtcgc cgcctacttc
     4621 cgtcgccggg atcgagacac cggcgatgtc cgcccaatca agcttcggcg gcgcctcggg
     4681 gagcatgctg atctgcacct cgcgcgcgaa gcgcagttcg tcgcggatac gcagttgctc
     4741 gcgcacttcc gagcggcgct gcaccccttc ctcgattgcc gtcctccggg cgttcctgct
     4801 gccgagcgcc ccgactgcga gggagccggc attgatgagc gagagcgcaa ccagaatccc
     4861 ggtcgtgttt cgcttcatcc gtccctcggg agccaggaag agcgccgcaa ggacgatggc
     4921 catcatcatc acgttgaggg cgatgcgctg cggcagcagc aaccgcagcg ccagcgttgc
     4981 gatgggaaag acgacacccc aggcgatcca tccgccgccg gacaatgcaa agtaggtg
//
LOCUS       DATLVW010000245         8382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_9650, whole genome shotgun sequence.
ACCESSION   DATLVW010000245 DATLVW010000000
VERSION     DATLVW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9650"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1055)
                     /locus_tag="VNN25_07835"
     CDS             complement(288..1055)
                     /locus_tag="VNN25_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91474.1"
                     /translation="MSSNAADQANFPTMIIPRGTEIRVNQSGQLSIKTPGNLVIQNSG
                     TYSEIESTNGSIRIEENVTVEAVSIRAGQACFIQGTLTAWRVHAKRITLEERARAFIM
                     LQESEHLEMAKNARLVGNFSNEKELFFMMGKFSPQLKELPGTVDMSTPATIEGRVPAA
                     WIPPGSQPGTTPTPTHREENGDRLRVAQSLLEREIARTDLAPAEAEALREVLYALRER
                     NVTRVGAIYRGAFAELKTQSEGVRQAFEQLDGYFGAR"
     gene            complement(1057..2091)
                     /locus_tag="VNN25_07840"
     CDS             complement(1057..2091)
                     /locus_tag="VNN25_07840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXH91475.1"
                     /translation="MIEIQIVPSDIRRRVRYVFFDRRRVVVALIFLSVLLAGLLGSMA
                     AAPTVIRRVYKDNYLKSMRDERDIQRERLQENVVQMTSVEKSLEEQRIRVEKLITIYG
                     LDRNLGTGGFSLPLHLKSDATDLHIDDARHHETALRTAMRRLQAQIELLASYETANAD
                     LVRHTPSILPLPSDQFVLTSPFGMRISPFTRASDFHKGMDLSAPTGTPVYATADGVVS
                     FAGRFPLRDSVAWWRFGNVVVVNHSDRFITIYGHCDTVKVHAGQQIKQGEVIASVGST
                     GWSTNSHLHYEVRSDFEQPGAYVPIDPRIYILNYQWPNEASLLMKARTSKDYKDFDPL
                     PTAFLGRRRV"
     gene            2378..4264
                     /locus_tag="VNN25_07845"
     CDS             2378..4264
                     /locus_tag="VNN25_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXH91476.1"
                     /translation="MEGSPDHKSSGRYLLILSLSALGVVYGDIGTSPLYALRECFHLK
                     HGVRATPDNVLGILSLICWSLIIVISLKYLVFVMRADNRGEGGILALMSLARTRESQS
                     TRRAILIALGLFGSALLYGDGMITPAISVLSAIEGLEVATPVFGPYVEIITVVVLIIL
                     FVVQRAGTAKVGALFGPVMMVWFGVISLLGIRWIAVYPRVLFAILPTHAVHFFIVNRL
                     QGFLVLGAVFLAVTGGEALYADMGHFGARPIRLAWFAVVFPSLLLNYFGQGALLLHDQ
                     SAVENPFFHMAPNWAKYPLVILATVAAVIASQAVISGAFSLTRQAVQLGYAPRIQIRH
                     TSAREIGQIYIPSVNWVLMIAAIGLVLAFKESSALAAAYGVAVTATMGITTALLAIVE
                     RERWNWSLIAVLSLTIPILIIDLSFFGANIIKVEEGGWFPLVIGALIFTAMTTWARGR
                     QILAERLKETTLSTDDLLRDLKGGRIPRVPGTAIFLSRQAEGVPTTLLHNLKHNKVVH
                     QRVILLSVSVEESPHLQDQERFEYKELGEGVSRLVLRFGFMEDPDLPDHLARLSKEIG
                     SFNPMTTSYFLGRETLVATKRPGMAIWRERLFGWMMRNSSSAAQYFSLPTNQIIELGA
                     QIEM"
     gene            complement(4416..5564)
                     /locus_tag="VNN25_07850"
     CDS             complement(4416..5564)
                     /locus_tag="VNN25_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH91477.1"
                     /translation="MSRIISISLCVAAVLGFGCRPGAERQTAAETNDIPVGVYAATSG
                     SEAAFGQATVQGEQLAAEEINNSGGVLGKKIRLVIEDDQGKAEEAASVVTKLITHDNV
                     LAILGENSSNQSLAAAPICQASKVPMISPSSTNPSVTKKGDYIFRVCFTDPYQGKALA
                     MFVRANLHATTAAILRDNKNDYSVGLSEVIRKEFETLGGKIVAEQSYSGGDSDFRPQL
                     TAIKSANPEVLFIPGFYTEVGQIAIQARDLGITYPMVGGDGWDSPTVIQIGGKSVDGC
                     YFSDHYFVGEPRPAVQKFVAEILKRSGKNPDANSALGYDALYTLTNAIKRAGSLDRKA
                     IRDQIAATKDYQGVSGVITMGPDRDPIKPVAMIKIDGGTTHFSGWIQP"
     gene            5603..6160
                     /gene="efp"
                     /locus_tag="VNN25_07855"
     CDS             5603..6160
                     /gene="efp"
                     /locus_tag="VNN25_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXH91478.1"
                     /translation="MQANDIRRGIVIVFNGDLCKVMEFNHHTPGNLRAMVQAKLRNMR
                     TGNQFEHRFRSQDEITQAYINQHEMEYMYSDGHAHHFMNTVSFEQVEISSDDLGDSAA
                     WLDAGVKLQVQFYEDKPIGVELPKTIRVRISETEPQVKGATASASYKPAKLENGVTVL
                     VPPFVNEGEEIIVDPSENRYLERAK"
     gene            6253..6693
                     /locus_tag="VNN25_07860"
     CDS             6253..6693
                     /locus_tag="VNN25_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91479.1"
                     /translation="MPSVNFLCLQCEKRLVRDAGEVSRPCDFCGVANGVTAPAEDSVI
                     DRCAACGHEELYFQKDFNRRTGIALVVLGSVFVPWTYGLSLLGVTILDYIVWRIVKDV
                     IVCYECQAVHRGYPPNPRLKPFDLVIHDRHVYGAAPPGAEEGHS"
     gene            complement(6769..7509)
                     /locus_tag="VNN25_07865"
     CDS             complement(6769..7509)
                     /locus_tag="VNN25_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91480.1"
                     /translation="MLLALFLIDLAAGLYLFLPLVGRRNAGVKFYRLILITTGVLALC
                     AYLADFAAHGGHFRIHALPAITLTIIVFATLRYPKRLIFRIPAALLGIVYLVLPVIAW
                     REGTHTSIPWSIAGALSSVALLGSVNLAMLLGHWYLVVRGMAIDPLKRLTIATLAATS
                     LKIVAVAIAIVAGGAWYEVAIRQGIFFWMRAGWGLMGPLLLYPMVWGTVKIRSTMAAT
                     GILYVDVVAVVIGEVLGAWLSAIAHIPV"
     gene            complement(7656..>8382)
                     /locus_tag="VNN25_07870"
     CDS             complement(7656..>8382)
                     /locus_tag="VNN25_07870"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXH91481.1"
                     /translation="SELQRPKPAEKPVTAAAIPERHRGDIVLILDDVGFDHQPLSQAM
                     TIDPNVNFAILPNGTRSTEFAAVLHDRGFELLCHLPMEPEGFPRISPGTGAVLTSMSD
                     AEIVRTTRANVGAIRFARGVNNHMGSRATADRRVMTDVLGALPQGMYFIDSKTTGNSV
                     AGSLAHSMNVKTASRNVFLDDAQDDAAIRHQLGELSRAAAAHGIAVGIGHMYPSTVRV
                     LTEDAPALRANGFRFLRASAAVE"
BASE COUNT         1543 a         2579 c         2666 g         1594 t
ORIGIN      
        1 atggcaggag gcgcaagcgt tgtcctgcgt gtactccgct gcgtgcatcg ccgggacgat
       61 gagaaacacg agcgatatcg ccagcagcgt cgtgcgggtc acaaacagat gatagggcgt
      121 aacgagccgt cattgtccgc acggagttcc cgcgagcaac cggcttcagc gggcgacagg
      181 ttaggttggc aagcgtccat cccgccgaac cagcttgaga atcccgagga caacgcgcga
      241 agcgcaatca accgacaacc gacaacgcgc gaagcgcaat accgaagcta tctcgcgccg
      301 aaatacccat ccaactgttc gaacgcctgc cgcacgcctt cgctttgcgt cttcagctcc
      361 gcaaatgcgc cgcgatagat ggctccaacg cgcgtcacat tccgctcgcg caacgcgtac
      421 agcacctcac gcagcgcctc ggcttcagcc ggggcaagat cggtgcgggc aatctcccgc
      481 tccagcagcg actgcgcgac gcggaggcga tcgccgtttt cttcgcgatg cgtgggcgtt
      541 ggcgtcgtgc cgggctgtga gcctggtgga atccaggctg cgggcacgcg tccttcgatc
      601 gtggccggcg tcgacatgtc cactgtgccg ggaagctctt tcaattgcgg gctgaacttg
      661 cccatcatga agaagagctc cttctcgttg gagaagttgc cgacgaggcg cgcgttcttc
      721 gccatctcga ggtgctcgga ctcctgcagc atgatgaagg cgcgggcacg ctcttcgaga
      781 gtgatgcgct tggcgtgaac gcgccaggcc gtgagcgtgc cctggatgaa gcaggcctgc
      841 ccggcgcgga tgctcaccgc ttcgaccgtc acgttctcct cgatgcggat cgaaccgttc
      901 gtcgactcga tctcggaata cgtcccggag ttctgaatca ccagattgcc gggagtcttg
      961 atcgagagtt gcccgctttg attgacacgg atctccgtgc cgcgcgggat gatcatcgtc
     1021 ggaaagttcg cctggtcggc ggcgttgctg ctcatctcac accctcctgc gcccgagaaa
     1081 cgccgtggga agcggatcga agtccttgta atccttgctc gtacgagcct tcatcaacag
     1141 ggatgcttca ttaggccact gatagttgag gatgtaaatg cgcggatcga tcgggacgta
     1201 ggcgccgggc tgctcgaaat cgctgcgaac ctcgtagtgg aggtgcgaat tcgtgctcca
     1261 gccggtcgag ccgaccgagg cgataacctc gccctgcttg atctgctgac cggcgtggac
     1321 cttgaccgtg tcgcagtgac cgtagatggt gatgaaacgg tcggagtgat tgacgacgac
     1381 gacgttgccg aagcgccacc acgccacgct gtcgcgcagc gggaagcggc cggcgaaact
     1441 caccactccg tcggcggtcg cgtagaccgg cgtgccggtg ggggcggaaa ggtccatccc
     1501 cttgtgaaag tcggaggcgc gcgtgaatgg cgagatgcgc atgccgaacg gtgacgtaag
     1561 cacgaactga tcggagggaa gtggaaggat cgagggcgtg tggcgcacga ggtcggcgtt
     1621 cgcggtctcg tagctggcca gaagctcgat ctgagcttgc agacgccgca tggccgtgcg
     1681 cagcgcggtc tcgtgatggc gtgcgtcgtc gatgtggaga tcggtcgcat cggacttcag
     1741 gtgcaggggc agcgagaaac cgccggtgcc gaggttgcgg tcgagaccgt agatggtgat
     1801 cagcttctcg acgcggatgc gctgctcttc cagactcttc tcgaccgacg tcatctggac
     1861 gacgttttcc tgcaggcgct cgcgctggat atcccgctcg tcgcgcatcg acttgaggta
     1921 gttgtctttg tagacgcgcc ggatgacggt gggggccgcg gccatggagc cgaggagacc
     1981 ggccagaagg accgagagga agatgagcgc tacgacgacg cggcgccggt cgaaaaagac
     2041 atagcgcacg cgacggcgga tatcgctcgg aacgatctgg atctcgatca tggcaccaac
     2101 cgctgggctg cgcgggtcgg tcaatataac acagggtgtg tcgcggaggt tgggcagtgg
     2161 gtggggggtg gggtgtgggg tgtaggaggt gggggcgacg gtgcgaagga atgcggctgc
     2221 cgaattgacc gatgaccaac cggcgggagt gtctggcctc atccgacacc cgactcccga
     2281 cacccgactc ccgacacccg acgccccaca tcacgggcgc tttcccactc cccacctccc
     2341 acaccccacc cccttccgtg ttaccttcgc gcgcccgatg gaaggttccc ccgatcacaa
     2401 gagcagcgga cgctatctcc tcatcctctc cctgagcgcc ctcggcgtcg tctatggcga
     2461 catcggtacg agtccgctgt atgcgctccg cgagtgcttt cacctcaagc acggcgtcag
     2521 agccacgccc gacaacgtcc tcggcatcct ctcgctcatc tgctggtcgc tcatcatcgt
     2581 catctcgctc aagtacctcg ttttcgtcat gcgcgccgac aaccgcggcg aaggaggcat
     2641 cctggcgctg atgtcgctgg cgcgcacgcg cgagtcgcag tcgacccgcc gggccatcct
     2701 catcgcactc ggcctcttcg gatcggcgct tctctacggc gacggcatga tcacacccgc
     2761 catctccgta ctcagcgcga tcgaagggct cgaagtggcg acgcccgttt tcggaccgta
     2821 tgtcgagatc atcaccgtag tcgtcctcat cattctcttc gtcgtgcagc gcgcggggac
     2881 agcgaaggtc ggcgctttgt tcggacctgt gatgatggtc tggttcggag tcatctcact
     2941 cctcggcatc cgctggattg cggtctatcc gcgcgttctt ttcgccatcc tgccgacgca
     3001 cgccgttcac ttcttcatcg tcaatcgact gcaaggattc ctcgttctag gcgccgtctt
     3061 cctggcagtc accggcggcg aagcgctcta cgccgacatg ggccatttcg gcgctcgtcc
     3121 catccggctg gcgtggttcg ccgtcgtctt tccttcgctc ctgctgaact acttcggtca
     3181 gggtgcgttg ctgcttcatg accaatcggc ggtcgagaat ccgttttttc acatggcgcc
     3241 gaactgggcc aaatacccgc tggtcatcct ggccacagtg gcagcggtca tcgcgtcgca
     3301 ggcagtgatc tccggcgcct tctcactgac gcggcaggcg gtgcaactcg gctacgcacc
     3361 gcgcatccag atccgccaca catccgcgcg cgagattggc cagatctaca tcccgtcagt
     3421 gaactgggtc ctgatgatcg cagcgatcgg cctcgtgctg gcgttcaagg aatcaagcgc
     3481 gctcgccgcc gcgtacggcg ttgcggtcac tgcgacgatg ggcatcacga cggcgctgct
     3541 ggccatcgtg gagcgagagc gttggaactg gtcgctcatc gctgtcctta gcctgacgat
     3601 tccgatcctc atcatcgacc tctcgttctt cggcgcgaac atcatcaaag ttgaagaagg
     3661 cggctggttc ccgctcgtga tcggagccct tatcttcacg gcgatgacga cctgggcgcg
     3721 cggccggcag attctggccg agcggctcaa ggaaacgacg ttgtcgaccg acgacctcct
     3781 gcgcgacctg aaaggcggcc gcataccgcg cgtgcccggt accgccatct tcctttcacg
     3841 gcaggccgag ggcgttccga cgacgctgtt gcacaatctc aaacacaaca aagtcgtaca
     3901 ccagcgcgtg atcctcctca gcgtgagcgt ggaggagtcg ccgcatctgc aggatcagga
     3961 gcgattcgag tacaaggaac tgggcgaggg cgtctcccgc ctcgttctcc gtttcggttt
     4021 catggaggat cccgacctcc ccgatcacct cgcccggctc tcgaaggaga tcggctcctt
     4081 caatccgatg acgacgagct acttcctcgg gcgcgaaaca ctggtggcga cgaaacgccc
     4141 cggcatggcc atctggcgcg agcgcctctt cggctggatg atgcgcaatt cgtcgagcgc
     4201 ggcacaatac ttctcgctac ccacgaacca gatcatcgag ttgggcgcgc aaatcgaaat
     4261 gtgattattg cgctgcgcgc gttgtcggtt gtcggttgat tgcccttcgg gcgttgtcag
     4321 agtcgtgaat cgcggtatgc gaatctgcgc gcgacaacgc gcgaagcgca actaaccgac
     4381 aaccgacaac gcgcggcccg ccagggccgc gcaattcaag gttgtatcca ccccgaaaag
     4441 tgcgtcgttc ccccatcgat cttgatcatc gcaaccggct tgatcggatc gcggtcgggg
     4501 cccatggtga tgacgccgga gacgccctgg tagtcctttg tcgcggcgat ctggtcgcgg
     4561 atcgctttgc gatcgaggga accggcgcgc ttgatcgcgt tcgtgagcgt gtagagcgcg
     4621 tcgtagccga gggcggagtt ggcgtcgggg ttcttgccgg agcgtttgag gatctcagcg
     4681 acgaacttct gcaccgccgg acgcggctcg ccgacgaagt aatggtccga gaagtagcag
     4741 ccgtcgactg acttgccgcc gatctgaatc acggtcggcg agtcccagcc gtcgccgcca
     4801 accatcggat aggtgatgcc gaggtcgcgg gcctggatag cgatctgccc gacctccgtg
     4861 tagaaaccgg ggatgaagag cacctccgga ttggccgact tgattgccgt caactgcggc
     4921 cggaaatcgc tgtcgccgcc gctgtagctc tgctccgcca cgatcttgcc gccgagcgtc
     4981 tcgaactcct tgcggatgac ttcggagagg ccaaccgagt agtcattctt gttgtcgcgc
     5041 agaatcgccg ccgtcgtcgc gtgcaggttg gcgcggacga acatcgccag cgctttcccc
     5101 tggtacgggt cggtgaagca gacccggaag atgtaatcgc ctttcttcgt gacgctgggg
     5161 ttcgtcgacg acggcgagat catcggcacc ttcgaggcct ggcagatcgg ggcagcggca
     5221 agcgactggt tggaggagtt ctcgccgagg atggccagga cgttgtcgtg cgtgatgagt
     5281 ttggtgacga ccgacgccgc ttcttccgct ttgccttgat cgtcttcgat gacgaggcgg
     5341 atctttttgc cgagcacgcc tccggaattg ttgatctcct cggcggcgag ctgttcgccc
     5401 tgaacggttg cctgcccgaa ggcagcctcc gatccgctcg tcgccgcata gacgccaact
     5461 ggaatatcgt tcgtctcggc ggccgtttgt cgttcggctc cgggacggca accaaacccg
     5521 agaactgccg caacacacag agaaatcgaa atgatccggc tcatagggcc gggagtctat
     5581 cagcgaaagg attcaccact tcatgcaggc caacgacatt cggagaggga tcgtcatcgt
     5641 gttcaacggc gatctctgca aggtgatgga gttcaatcac cacacgcccg ggaatctccg
     5701 cgcgatggtg caggcgaagt tgcgcaacat gcgcaccggc aaccagttcg agcaccgctt
     5761 ccgctcgcag gacgagatca cgcaggcgta catcaaccag catgagatgg agtacatgta
     5821 ctccgatggc catgcccatc acttcatgaa caccgtgagc ttcgagcagg tcgagatctc
     5881 ttccgacgac ctcggcgact ccgccgcgtg gctggatgcc ggcgtgaagc tgcaggtgca
     5941 gttctacgaa gacaagccga tcggcgtcga gctcccgaag acgatccgcg tccgcatctc
     6001 cgagaccgag ccgcaggtga agggcgccac ggcctccgcg tcgtacaagc ccgccaagct
     6061 cgagaacggc gtgacggtgc tcgtgccgcc gttcgtcaac gaaggcgaag agattatcgt
     6121 cgacccgagc gagaaccggt acctcgagcg ggcgaagtag cgtgggcgga gatcagtgga
     6181 ttagagatca gaggctcggg atcgagcctc tttctgatcg acgaatccac taatctactc
     6241 gctccactgc ccatgccgtc cgtcaacttc ctctgtcttc aatgcgaaaa gcgcctcgtg
     6301 cgcgatgccg gtgaggtgtc gcggccgtgt gatttctgcg gcgtggcgaa tggcgtgact
     6361 gcgccggcgg aagactccgt catcgaccgc tgcgccgcgt gcggacacga agagctctat
     6421 tttcaaaagg acttcaaccg caggaccggg attgcgctcg tcgtcctcgg ctcggtcttc
     6481 gtcccctgga cctacggtct ctccctcctc ggcgtcacga tcctcgacta catcgtctgg
     6541 cgcatcgtga aggacgtcat cgtctgctac gaatgccagg cggtgcatcg cgggtatccg
     6601 cccaatccgc gcctcaagcc gttcgatctg gtcatccacg atcggcatgt ttacggcgct
     6661 gcgccacctg gggccgagga aggacattcg taaaggcttt aaccacagag acacagagga
     6721 cacagagagt tctctgtgct ctctgtgact ctgtggttca aagaaagtct aaacagggat
     6781 atgcgcgatt gcgctcagcc atgcgccgag cacttcgccg atcacgacgg cgaccacatc
     6841 gacgtacagg atgccggtcg cggccatcgt cgagcggatc ttcacggtgc cccacaccat
     6901 cggatagagg agcagtggac ccatcaatcc ccacccggcg cgcatccaga agaagatccc
     6961 ctggcggatg gctacttcgt accaggcgcc gccggcgacg atcgcaatgg cgacggcgac
     7021 gatcttcaag ctcgtcgccg ccagcgttgc gatcgtgagc cgtttcagcg gatcgatggc
     7081 catcccgcgc acgaccaggt accagtgccc gaggagcatg gccaggttga cgctgccgag
     7141 gagtgccacg ctcgatagcg caccagcgat ggaccagggg atcgacgtgt gcgttccctc
     7201 gcgccatgcg atcacgggaa gaacgaggta gacgatgccg aggagtgcgg ccgggatgcg
     7261 gaagatcagg cgcttcggat agcgaagggt cgcgaacacg atgatcgtca gggtgatggc
     7321 tggcagcgca tggatcctga agtggccacc gtgcgcggcg aaatcggcga ggtaggcgca
     7381 gagggcgagg acgccggttg tgatcaggat caggcggtag aacttgacgc ctgcgttgcg
     7441 acggccgacg agcgggagaa agaggtagag gccggcggcg aggtcgatca ggaagagggc
     7501 gagcagcatg gagcgcggag tgtaccgttg ccttcaggtt gagcaggctt cggttttacg
     7561 tagggtcggt tttacgtagg tcggactctc tagtccgaca tcacgtgcag tcggactgga
     7621 gagtccgacc tacgtaaaac ctacgtaaac cgcgcctact cgacggcggc gctggcgcgg
     7681 aggaagcgga agccgtttgc gcgcaaggct ggcgcgtcct cggtcaggac gcgcaccgtc
     7741 gacggataca tatggccgat gccgaccgcg atgccgtgcg cggcagcggc tctcgacagt
     7801 tcgcctagtt gatggcggat cgccgcgtcg tcctgcgcgt cgtcgaggaa gacgttgcgc
     7861 gaggccgtct tcacgttcat cgaatgcgcc agcgatcccg ccacggaatt gcccgtcgtc
     7921 ttcgaatcga tgaagtacat cccttgcggc agcgcgccga gtacgtccgt catcacgcgc
     7981 cggtccgcgg tggcgcgcga gcccatgtga ttgttcacgc cgcgagcgaa gcggatggca
     8041 ccgacgttcg cgcgtgttgt tcgcacgatc tcggcgtccg acatcgacgt gagcacggcc
     8101 cccgttccgg gcgagatgcg cggaaagccc tccggctcca ttggtagatg gcaaagcagc
     8161 tcgaagccgc ggtcgtgaag taccgccgcg aattccgtgg agcgcgtgcc gttcgggagg
     8221 atcgcgaagt tcacattcgg atcgatcgtc atcgcctgcg agaggggttg atgatcgaag
     8281 ccgacgtcat cgagaataag aacaatgtcg ccgcggtgcc gttccgggat ggccgcggcg
     8341 gtgacgggtt tctcggcggg tttcggacgc tgaagctcgg ac
//
LOCUS       DATLVW010000246         9973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_298763, whole genome shotgun sequence.
ACCESSION   DATLVW010000246 DATLVW010000000
VERSION     DATLVW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298763"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..6608
                     /locus_tag="VNN25_07875"
     CDS             <1..6608
                     /locus_tag="VNN25_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXH91482.1"
                     /translation="LVTSCPTITVTNPATTTGTVGVAFSQTFTQSGGIGTTTFSESGG
                     LPSGLTFHTATGVLDGTPSQFGTFGITVTATDSHNCQGTGSIYTLVISCPTITVTNPG
                     VNTGTVDTPFSQTFTQSGAFGTATFTTASTLPAGLTLSTAGVLSGTPTAPVVSVPIVV
                     TVTDGAGCTGTGSTYSLTINCQTITVTKPVVTTGTVDAPFSQTFTQSGVGTHTPASFA
                     INSGTLPAGLTLSNAGVLSGTPTQQGSFPITVKVTDVNGCSGVSSTYNLFINCQTITV
                     TNPLTNTGMAGVAFSQTFTQSAAHGTATFTTASTLPTGFSLSNAGVLSGTTTQHGLFS
                     IVVTVTDANGCTGTGSTYSLNIACNPISVTNPVNTNGTVAVAFSETFTSSGTLGTPSY
                     TTASTLPTGLTLHAATGILDGTPTQAGNFPIVVTATDSNGCTGTGATYTLHITCNVIT
                     VTPPGVTSGTAGVNFDQAFSQSGGNGTINWTETGDTLPAGIVLDSTTGHLHGSTTSTG
                     SFNITVTATDANGCSGSTPYTLTINCQTVTVTNPAQTSVQAGTTLDVVFTASGILGTV
                     TWSETGDTLPGTIALNASTGHLSGTTNSIGTFNIVVKALDSNGCFGTSNYTLTVTCPT
                     ITVTRTGGGSFPAATFNVGYPAGNTFTATGQSGTIHWAFPAGDQPPGINLTIVAGSLS
                     GIPSATGTFNMTPVATDQSTGCTGTATFPFSILPNMTADSYSNLVNNTQAYVTGGSTS
                     APATPAVQLSGTIVSNDTPASGVTVAASSIGTFATTQGGSVTIAADGTFKYTPPLTSI
                     SSDTFNYTGSSNTGTIPSIPQYAASTGPATNTGTVTLNLVNRVWYVKNNAAGGGNGQS
                     QSPFNTLAAAAAASAASDIIFVYNGDGTTANQTAGITLKSNQQLIGEGVALVVNSNTL
                     VAAGTKPQISNTTAASDAVTINDGNTVKGLTITGATRDGISSVVTHAGFTADTITIQN
                     NTSSGLHLTSMTGTVTVTNATISGNATGLDVNNGTAAITLDNTNTITSGAAGRTVSIQ
                     NRPVSAGTIALGATINDAGTGILVNNNASGTINFTGSQTPNVGGANTAVNLTTNTGTT
                     INFSGTLNATSATGSGFNATGGGTLSVTGTANVTTGAAGTGVNINAVTVGASGVTFNS
                     VNTTGATTGVSLTSLGNGNVTINGGTISGGTTGLSLNTLGTSTVTLAGVTLTGSTTAI
                     SGTTFGTLSIGAAVNVSGATALNLATGAVTGTFANVSSTGGTNGVNLNAVTGTWGATA
                     GSLTGATGATFNVTGGSGGTISWGATINQAGGANAVTIASSNSNTINFSANVTTSGTS
                     TGIGISGSTGTYNFTGAANTITGTGGGITISSEASGTISFGSGTSINGATNTFSVTSS
                     SANITYSGTITDNVNGGTLLNIATYSTGTLTMNGTSLSGNVNGINGVISALSAITGTV
                     VINNLSLTSGNNNFSNTLVAITGVNTGGSITFNHLTLSATGTGHTGKGLTMVGNGTLA
                     ITATGGASSIDVSSTALDLNGQALGASALATLNSSGGVNGILLTNVTGGTMTITGGTI
                     AGNTGGAFVVSGGTPSVSYGGTITQNTAGQRAVDLQTRTGGTITFSGLITSSGGNGVF
                     MNNAAGGSTTVFSGGLTLSTGGADAFTATGGGIVNVSGTNNVTTAAGKGINWNTDTSS
                     TGVTFNNVTSTTGAAVTIVSSGGSNFTFNDITSTTGTAVTVTTATGVFSFHKISSNGA
                     AKGITVSALTGTGSFTVTGTGGSGLCDSTHIGATDCQGGTIQGGTTRGAEFNNARAVT
                     LNNMYFKGNGTTAVACGDDVSGSSNTTCDAAIYLVTAASPTLNKIYVDGTGSNDSGII
                     GNAVSAMTVATLEIANFTNVAKTPFNFQNLTGTTGVTGLNVHNNSGLHNVMITNNTGT
                     ANITLTSPTLTSNSGSGNPDQLQVNSYIAGTTLNVTATNVNITASSANTGNGVMFQAN
                     SGSSMTSILNGGVVTGTNGLLMQETGTNTTFNFTVTGLTSVTTHNLGSNAITVGKANG
                     TNGVFTGTVTNNVITSATCGGGCAGIKVASFGVSGASTVNVSNNNISGVDAQGIWIVG
                     GQGGSSLTATVQTNNIHNPVGSPNTSYAIDVYPGTTSGDAVCFALNLGDMTATHTVPA
                     NRNTITGTWQSGGNPIEAAIFNSSTFKLLNYSGSTDANAAAWIAASNGGLGTDAFHLG
                     TNQFTSGASCP"
     gene            6707..7213
                     /locus_tag="VNN25_07880"
     CDS             6707..7213
                     /locus_tag="VNN25_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HXH91483.1"
                     /translation="MGTPFLSEIRIMAFNFAPKGWAGCNGQFLPINQNQALFSLLGTM
                     YGGNGQTTFALPDFRGKAPVHIGSGITQGQIGGQESHTLTQSEMPSHNHFVQANLQPN
                     PADSAIPSPNLLGTLTTPIYGPLSSLTTLNPASVTNTGGSQPHQNMQPYIVLNFCIAL
                     QGAFPSQN"
     gene            7227..7748
                     /locus_tag="VNN25_07885"
     CDS             7227..7748
                     /locus_tag="VNN25_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HXH91484.1"
                     /translation="MSSPYIGEIRMFGGNFAPAGWAMCQGQLMPISQNDTLFNLIGTT
                     YGGDGQETFGIPDVQGRLPIHMGTNPQTGTTFQIGEEAGVESVTLTTNQMPQHNHALV
                     ASTGSGTTGVPTGTVLASNLQVSLYSQFPPTNNMNAAGITNVGGSQPHENMMPFLVVT
                     FIISLFGIFPSQN"
     gene            7767..8294
                     /locus_tag="VNN25_07890"
     CDS             7767..8294
                     /locus_tag="VNN25_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HXH91485.1"
                     /translation="MSSPFVAEIRMWGCNFAPTGWAQCNGQLLPISQNTALFSLLGTM
                     YGGDGKSTFALPDMEGNAPMNQGQGSGLSDRFIGEQSGVEFVTLIQTEMPVHNHFLVG
                     HNAHSGANPSPAPDQALSKSAGGAAYTAQSANLTQMDFQSLSIAGSSLPHNNMQPYLC
                     VNFCIAMQGVFPPRS"
     gene            8346..8834
                     /locus_tag="VNN25_07895"
     CDS             8346..8834
                     /locus_tag="VNN25_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH91486.1"
                     /translation="MDNLPDSIRLRPRRDDDRPFLAMLYASTRQQEMAVIDWTDEQKQ
                     AFLQWQFDAQTVHYDEQYDGADFLVIEQDGTPIGRLYIDRREDQIEIVDIALVPECRG
                     SGLGSCLLRDVMREAEESRRSVKIYVENFNPARRLYDRLGFQHVDSNGVYHIMTWMAG
                     GA"
     gene            complement(8835..9152)
                     /locus_tag="VNN25_07900"
     CDS             complement(8835..9152)
                     /locus_tag="VNN25_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91487.1"
                     /translation="MDLSEVTLETAQPLVGTLFQVTLDDGNTLEMKLIDAAPFELPRR
                     PARGSRQPKRLPFALYFLGPRDPILPQHMYHFRSAGVELESLFIVPVGRDDEGTEYEA
                     VFA"
BASE COUNT         2089 a         3466 c         2812 g         1606 t
ORIGIN      
        1 ccctcgtcac gtcctgcccg accatcacgg tcaccaaccc ggccacgacc accggcacgg
       61 tcggtgtggc gttcagtcag accttcacgc agtccggcgg catcggcacg accacgttca
      121 gcgagagcgg cggtttgccg agcggtctga ccttccatac cgccacaggc gttctcgacg
      181 gcacgccgtc gcagttcggc acgttcggaa tcacggtcac cgccaccgac tcgcacaact
      241 gtcagggcac cggctcgatc tacaccctcg tcatctcgtg cccgaccatc accgtgacca
      301 acccgggtgt gaacaccggc acggtcgaca caccgttcag ccagaccttc acccagagcg
      361 gagcgttcgg tacggccacc ttcacgaccg cctcgaccct tcctgccggt ctgacgcttt
      421 cgaccgctgg cgttctctcc ggtacgccga cagccccggt ggtgtccgtc ccgatcgtcg
      481 tcaccgtcac tgatggcgcc ggctgcaccg gcaccggttc gacctactcg ctgaccatca
      541 actgccagac cattaccgtc accaagcccg ttgtaacgac cggcaccgtt gacgcgccgt
      601 tcagtcagac gttcacccag agcggcgtcg gcacccacac cccggcttcg ttcgccatca
      661 acagcggcac gctgccggcg ggcctgacgc tttcgaacgc cggcgtactc tcgggcacgc
      721 caacccagca ggggtcgttc ccaatcaccg tcaaggtgac ggacgtcaac ggctgctccg
      781 gcgtcagttc gacctacaac ctgttcatca actgccagac catcaccgtc acgaaccccc
      841 tcacgaacac cggcatggca ggagtggcct tcagccagac cttcacccag agcgccgcgc
      901 acggcacggc gaccttcacg actgcgtcga cgctgccgac cggcttctcg ctctctaacg
      961 ctggggtcct ttccggcacg accacgcagc acgggttgtt ctcgatcgtg gtcacggtca
     1021 ccgacgccaa cggctgtacg ggcacgggat cgacctacag cctgaacatc gcctgtaacc
     1081 ccatcagcgt caccaacccg gtcaacacca acggcacagt ggccgtggcc ttcagcgaga
     1141 ccttcacctc gtccggtacg ctcggcaccc ccagttacac cacggcttcg acactgccaa
     1201 ccggcctgac gctccatgca gctactggca tcctcgacgg cacgccgacg caggcgggga
     1261 atttccccat cgtcgtcact gcgaccgaca gcaacggttg caccggcacc ggcgccacgt
     1321 acacgctgca catcacctgc aacgtcatca ccgtaacgcc tccgggtgtg acctcgggta
     1381 ccgcaggcgt caacttcgac caggccttca gccagtcggg cggcaacggc acgatcaact
     1441 ggacggagac gggtgacacc ctgcccgccg gaatcgtcct cgactccacc accggtcatc
     1501 tgcacggctc gacgacctcg accggctcct tcaacatcac ggtcacagcc accgacgcca
     1561 acggctgctc cggctcaacg ccctacacgc tgacgatcaa ctgccagacg gtcaccgtca
     1621 ccaacccggc tcagaccagc gttcaggccg gcaccacgct cgacgtcgtc ttcacggcca
     1681 gcggcatcct cggcaccgta acctggagcg agaccggcga cacactgcca ggtaccatcg
     1741 cattgaacgc gtccaccggc catctgtccg gcacgaccaa ctcgattggt acattcaata
     1801 tcgtcgtcaa ggccctcgac tcgaacggct gcttcggaac gtcgaactac accctcaccg
     1861 tcacctgccc gaccatcacc gtcaccagga ccggcggcgg ctcgttcccg gcggcgacgt
     1921 tcaacgtcgg ctatccggcc ggtaacacct tcaccgcgac cggccagagc ggcacgattc
     1981 attgggcgtt cccggccggc gaccagccgc ccggcatcaa cctgaccatc gtcgccggat
     2041 cgctgagcgg tattccgtcc gcgacgggca cattcaatat gactccggtc gcgaccgatc
     2101 aatccaccgg ctgcaccgga acggcgacgt tcccgttctc gatcctgccg aatatgacgg
     2161 cggactcgta cagcaacctc gttaacaaca cccaggcgta tgtcaccggc ggatcgacct
     2221 cggcgccggc caccccggcc gtacagctct ccggcacgat cgtctccaac gacacgccgg
     2281 ccagcggcgt caccgtcgcc gccagcagca tcggcacctt tgccaccacg cagggcggca
     2341 gcgtcaccat cgcagccgac ggcacgttca agtacacccc gccgctcacg tcgatctcgt
     2401 cggacacgtt caactacacc ggcagcagca acaccggcac gatcccgagc atcccgcaat
     2461 acgcggcttc cacaggacca gccacgaaca ccggcacggt cactctgaat ctcgtcaacc
     2521 gcgtctggta cgtgaagaac aacgcggcgg gcggcggcaa cggccagtcg caatcgccgt
     2581 tcaacacgct cgcggcagcg gcggcggcat cggccgcaag cgacatcatc ttcgtctaca
     2641 acggcgatgg cacgacggcc aatcagaccg ccggtatcac gctgaagtcc aaccagcagc
     2701 tcatcggcga aggcgtagcg ctggtcgtca acagcaacac gctcgtggct gcaggaacga
     2761 agccgcagat ctcgaacacg actgccgcga gtgacgcggt cacgatcaac gacggcaaca
     2821 cggtcaaggg tctgaccatc accggcgcca cacgcgacgg tatctcctcg gtcgtcaccc
     2881 acgccgggtt caccgccgac accatcacga tccagaacaa caccagctcc ggcctgcacc
     2941 tgacgagcat gaccggcacg gtgaccgtca ccaacgcgac gatcagcggg aacgccacgg
     3001 gcctggatgt caacaacggc accgcggcga tcacgctcga caacacgaac acgatcacct
     3061 ccggagcggc cggacgcacg gtctcgattc agaatcgtcc cgtgtcagcg gggacgatcg
     3121 ccctcggcgc cacgatcaac gacgccggca ccgggatcct tgtcaacaac aacgcctcgg
     3181 ggacgatcaa cttcaccggc agccagacgc cgaatgtcgg aggggcgaat accgccgtca
     3241 acctgacgac gaacaccggc acgaccatca acttcagcgg cacgctgaac gccacgagcg
     3301 cgaccggatc aggtttcaac gctaccggcg gcggcacgct cagcgtgacc ggcactgcga
     3361 acgtcaccac cggcgctgcc ggcaccggcg tgaacatcaa tgcagtgacc gtcggcgcgt
     3421 cgggtgtgac cttcaacagc gtcaacacca caggcgccac cacgggcgtc tctctgacgt
     3481 cgctcggcaa cggcaacgtg accatcaacg gcgggacgat cagcggcggt acgaccggtc
     3541 tctcactcaa tacactcggc acctcgaccg ttacgctggc tggtgtgacg ctcactggct
     3601 ccacgaccgc gatcagcggc accaccttcg gcacgctctc gatcggcgcg gcagtcaacg
     3661 tctcaggcgc caccgcgctg aacctcgcca ccggcgctgt caccggcacg ttcgcgaacg
     3721 tcagctctac aggcggcacg aacggtgtga acctcaacgc cgtcaccggt acgtggggtg
     3781 ccacggcggg ctcgctcacc ggagccaccg gcgcaacgtt caacgtcacc ggcggcagcg
     3841 gcggcacgat ctcgtggggc gccacgatca accaagccgg tggcgcgaat gccgtcacca
     3901 tcgcctcgag caactcgaac acgatcaact tcagcgccaa cgtgaccacg agcggcacga
     3961 gcaccggcat cggcatcagc ggcagcaccg gcacgtacaa cttcaccggc gcggcgaaca
     4021 ccatcaccgg caccggaggc ggcatcacca tcagcagtga ggcatccgga acgatttcgt
     4081 ttggctccgg cacgagcatc aacggcgcca cgaacacgtt ctcggtgacg agctcctcgg
     4141 cgaacattac ctatagcggc acgatcaccg acaacgtcaa cggcggcacg ttgttgaaca
     4201 tcgccaccta cagcacaggc actctgacga tgaacggcac ctcgctcagc ggcaacgtca
     4261 atggcatcaa cggcgtgatc tccgcactga gcgctatcac cggcaccgtg gtcatcaaca
     4321 acctgagcct gacctcgggt aacaacaact tcagcaacac cctggtagcc atcaccggcg
     4381 tgaacaccgg cggctcgatc acgttcaatc acctcacgct atcggccact ggaaccggcc
     4441 acaccggcaa gggcctcacg atggtcggca acggtacgct cgcgatcact gcgacgggcg
     4501 gcgcgagcag catcgacgtc agcagcacgg ctctcgatct gaatgggcag gcgctcggtg
     4561 cgtcggctct ggccacgctg aactcgagcg gcggcgtgaa cggcatcctg ctgaccaacg
     4621 tcaccggcgg cacgatgacg atcacgggcg gcacgattgc cggcaatacc ggcggcgcgt
     4681 tcgtggtcag cggcggcacc ccatcggtca gctacggcgg cacgatcacg cagaacacag
     4741 cagggcagcg agcggtcgac ctccagacca ggaccggtgg aaccatcacc ttctcgggtc
     4801 tcatcaccag ctcgggcggc aacggtgtct tcatgaacaa cgctgcgggc ggttcgacca
     4861 cggtcttcag cggtggcctg accctgagca caggcggcgc cgatgccttt accgccaccg
     4921 gcggcggcat cgtcaacgtc agcggcacga acaacgtcac caccgccgcc ggcaagggca
     4981 tcaactggaa caccgacacg tcgagcaccg gcgtgacgtt caacaacgtc acatcgacga
     5041 ccggcgccgc ggttacgatt gtcagcagcg gtgggagtaa cttcaccttc aacgacatca
     5101 cctcgacgac aggcacggcc gtcaccgtca ccaccgccac cggagtcttc agcttccaca
     5161 agatcagctc caatggcgcc gccaaaggca tcaccgtctc agccctgacc ggcacgggaa
     5221 gcttcaccgt caccggtacc ggaggctccg gtctctgcga ttcaacgcat atcggcgcca
     5281 cggattgtca gggcggtacg attcagggcg gcaccacgcg cggcgccgag ttcaacaacg
     5341 ccagggccgt cacactcaac aacatgtact tcaagggcaa cggcacgacg gccgtcgcct
     5401 gcggagacga tgtctcgggt agctcgaaca cgacctgcga cgccgccatc tatctggtaa
     5461 cggcggccag tcccaccctc aacaagattt acgtcgacgg cacgggatcg aatgactccg
     5521 gcattatcgg caacgccgtc agtgcaatga cggtcgctac cctcgagatc gcgaacttca
     5581 ccaacgtggc caagacgccc ttcaacttcc agaacctgac cggcaccacc ggcgtcaccg
     5641 ggctgaacgt ccacaacaac agcgggctgc acaacgtgat gatcaccaac aacacgggca
     5701 cggcgaacat caccttgacc agtccgacgc ttacgagcaa cagcggaagc ggcaaccccg
     5761 accagctcca agtcaattcg tacatcgcag gtacgaccct gaatgtcacg gcaaccaatg
     5821 tgaacatcac cgcgagctcg gccaacacgg gcaatggcgt catgttccag gcgaactccg
     5881 gctcgtcgat gacgagcatt ctcaatggcg gcgtggtgac cgggacgaac ggcctcctca
     5941 tgcaggagac gggtaccaac accacgttca acttcacagt gaccggcctg acgtccgtca
     6001 ccacgcacaa cctcggttcg aacgcaatca cagtcggaaa ggccaacggg acgaatggcg
     6061 tattcaccgg cacggtgacc aacaacgtca tcaccagcgc cacctgcggc ggcggctgcg
     6121 cgggcatcaa agtggcgtcg ttcggagtga gcggcgcttc tacggtcaat gtttcgaaca
     6181 acaacatctc cggtgtcgat gcccagggca tctggatcgt tggcggccag ggtggtagct
     6241 ccctcacggc gacggtgcaa acaaacaaca ttcacaatcc ggttggaagc cccaatacgt
     6301 cgtacgcgat cgacgtctac cccggaacca cgagcggcga tgcggtctgc ttcgccctga
     6361 acctcggcga tatgaccgcg acccataccg ttccggcgaa ccgcaacacc atcaccggca
     6421 cctggcagag cggcggcaac ccgatcgagg ccgccatctt caacagcagc actttcaagc
     6481 tgctcaacta ctcgggttcc accgatgcca acgcagccgc gtggattgcg gccagtaacg
     6541 gcggtctagg cacggacgcg ttccacctcg gaacgaacca gttcacctca ggagcgagtt
     6601 gtccatagta gaagtagcca aacgcagcgg ccccacatga tgcgcctcac gcgagtgagg
     6661 cgcatccaag ctccaaagat agagagaacg aaaaggaggt agtcgtatgg gtacaccttt
     6721 cctgtcagag atacgcatca tggccttcaa ttttgctcca aagggttggg ctggctgcaa
     6781 cggccagttt ctccccatca accagaacca ggcgctcttt tccctcctag gcacgatgta
     6841 cggcggcaac ggacagacga cgttcgcgct gcccgacttc cgcggcaagg cccccgttca
     6901 catcggatcg ggcatcaccc aagggcagat cggcggtcag gagtcgcaca ctctgaccca
     6961 gtcggagatg ccctcccaca accacttcgt gcaggccaat ctccaaccca accccgccga
     7021 ctcggcaatc cccagtccca acctcctcgg caccctgaca acaccgatct acggccctct
     7081 gagtagtctg accacgctca atccggcgtc ggtcacgaat acgggcggtt ctcaaccgca
     7141 ccagaacatg cagccgtaca tcgttctcaa cttctgcatt gcgctgcagg gcgctttccc
     7201 gtcgcaaaac taaggagggt gaagaaatga gcagtccata tatcggtgaa atccgcatgt
     7261 tcggtggcaa ctttgcgccg gcgggctggg caatgtgtca aggccagctc atgcccatct
     7321 cccagaacga cacgctgttc aacctgatcg gcacgaccta cggcggcgac ggccaggaaa
     7381 ccttcggtat tcccgacgtc cagggccggc ttcccatcca tatgggaacg aacccgcaga
     7441 ctggtactac ctttcagatc ggggaagagg ccggcgtcga gtcggtaact ctcaccacca
     7501 atcagatgcc gcaacataac cacgccctgg tggcttcgac gggcagtgga accacgggcg
     7561 tccccacggg gaccgtcctc gcctcgaatc tgcaggtgtc gctctattcg cagtttccgc
     7621 cgacgaacaa catgaacgcc gctggcatca cgaacgtggg aggcagccag ccgcacgaga
     7681 acatgatgcc gttcctggtc gtcacgttca tcatttcgct cttcggcatc tttccgtcgc
     7741 agaactagag aagggagaaa acaaaaatgt cttcaccatt tgtggcagag attcgcatgt
     7801 ggggctgcaa cttcgcgccc accggctggg cgcagtgcaa cggccagttg ctccccatct
     7861 cccagaacac agccctcttc tcgctgctcg gaacgatgta cggcggcgat gggaagtcga
     7921 ccttcgcact tcccgatatg gagggtaacg cgccgatgaa ccaggggcag ggttcgggcc
     7981 tctccgaccg gttcatcgga gagcagagcg gtgtcgagtt cgtgacgctg atccagaccg
     8041 agatgccggt ccataatcat ttcctcgtcg gtcacaatgc ccactcgggg gcgaatccct
     8101 cgcctgcacc cgatcaggcg ctcagcaagt cggctggcgg cgccgcgtat acggcgcagt
     8161 cggcaaacct gacccagatg gacttccagt ccctgagcat tgccggctcg tcgctccccc
     8221 acaacaacat gcagccttac ctctgcgtca acttctgcat cgccatgcag ggcgtcttcc
     8281 cgccccgcag ctaaacgggt ctccttcggt gcggggcttc gcaggccccg caccgcctcg
     8341 tactcatgga caatctgccc gactcgatcc gcctccgccc gagacgcgac gacgatcgtc
     8401 ccttcctcgc catgctgtac gccagcacgc ggcagcagga gatggctgtg atcgactgga
     8461 cggacgagca gaaacaggcc tttctccagt ggcaattcga cgcccagacc gtccactacg
     8521 acgagcaata cgacggcgcc gacttcctcg tcatcgaaca ggacggcact cctatcggcc
     8581 ggctctacat cgaccgccgg gaggaccaga tcgagatcgt cgatatcgcc ctggttcccg
     8641 agtgccgcgg ttccggcctg ggatcatgct tgctgcgcga cgtgatgcgc gaggcggaag
     8701 agagccggag aagcgtgaag atctacgtcg agaatttcaa tccggcccgc cgcctgtacg
     8761 accgcctcgg attccagcac gtcgactcaa acggcgtcta ccacatcatg acgtggatgg
     8821 ccggcggcgc ctgatcaggc gaagaccgcc tcgtactccg tcccttcgtc atcgcgcccg
     8881 accggcacga tgaagaggct ctcgagctcg acgccggcgc tgcggaagtg gtacatgtgc
     8941 tgcggaagga tcggatcgcg cggcccgaga aaatagaggg cgaacggcag ccgcttcggt
     9001 tgccgcgatc ctcgcgccgg ccgcctgggt aactcgaacg gtgcagcgtc gatcagcttc
     9061 atttccagtg tgttgccgtc gtccagggtt acctgaaaca acgtaccgac gagcggctgg
     9121 gccgtttcga gggtgacctc actcaagtcc atcgacgctc ctccagtgga acgttttagc
     9181 acactcgacg gtgccacgaa acgattgtgt tgcgcttgcc gtgggtggag ggatttcacg
     9241 ctgaacgtct ttttgtgggc gagaaggtgg caagccggat gagggggcgt tcgcctctcg
     9301 cgatgacgtc gccgaagcca ccgtgagtcc acgcgtgtca taacctcatg ccagtcgagt
     9361 gagtgcgcaa gagggaagcc gtacgggtta gcgcaaagtg agtgctggct ttgcgaagtt
     9421 gtcggcacct tccaatcgag cgccttctcc cccacgtctt ttctgtgggg gagaaggtgg
     9481 cgaagccgga tgagggggcg ttcggctttc gcgatgacgt tgccgaagcg ccgtgagtcc
     9541 acgcgtgtca taacctcatg ccagtcgagt gagtgcgcat gagggaagcc gtacgggtta
     9601 acgcgaaatc gagtgctggc ttcgcgaagt tgtcagcacc ttccaatcga gcgccttctc
     9661 ccccacgtct tttctgtggg ggagaaggtg gcgaagccgg atgagggggc gttcgcatct
     9721 cgcgatgacg ttgccgaagc accgtgagtc cacgcgtgtc ataacctcat gccaatcgat
     9781 tgcgcgatac cccggcgctc gatacacggg gtggcgagaa ttctcggagt gaacacatat
     9841 gacgtacgaa tgagtcgcat gcgagtgctg actcgccgag gttgtcaaaa gcactcaaga
     9901 agaattaaca agggtgtggg ctcgaggtgc aagaatgtga gaaatgcggg ttggagaaga
     9961 tccgcacctc gtg
//
LOCUS       DATLVW010000247        10815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_9732, whole genome shotgun sequence.
ACCESSION   DATLVW010000247 DATLVW010000000
VERSION     DATLVW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9732"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(480..1868)
                     /locus_tag="VNN25_07905"
     CDS             complement(480..1868)
                     /locus_tag="VNN25_07905"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXH91488.1"
                     /translation="MRRIVTALLALAFTAVLSAQELPADMRASIDKSANDILAKTGAP
                     SASIAVVRDGKIVYTNAYGTANLETKLAASPKMRYSIGSISKQFTATALLLLAEEGKL
                     SLDDKVIRWLPDLTRAGDVTIRQILSMTSGYQDFWPQDYVMPMMMQPVTAPEIVAGWA
                     KKPLDFEPGAKWQYSNTNYVIAGMIAEKVAGEPLLDFLQKRVFVPLHMTTVSNTDAAP
                     LGPEDPMRYLRYALGPLRPAPKEGRGWMFAAGELAMTAHDLALWDISMIDQTVLKPES
                     YRVLEAEVPMTNGVGSRYGLGVSVGIANGKRIISHGGEVSGFTARNEVYPDDRVAIVV
                     LTNMDATSASEQIAGGISRAIFASTDPATPKTTEQMRKIFDELQKGRIDRSQFTSNAN
                     AYFDEQAIKDFASSLAPLGTPQDFSQAGQSLRGGMTLRRYTIKFPNKTLRLTTFIMPD
                     GKIEQYQIAAAE"
     gene            complement(2055..4157)
                     /locus_tag="VNN25_07910"
     CDS             complement(2055..4157)
                     /locus_tag="VNN25_07910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXH91489.1"
                     /translation="MISACVLEAQTAAEPARQNPLSTASPLLYQAPPFDQIKDTDYQP
                     AIEEGMKVQLREIDAIANDPAPPTFANTIEAMERSGALLTRASKIFFNLTQSNTNETF
                     QKIEAEESPKLAAHGDAIYMNPKLYARVKTLYDSRAASGLDAESKFLIERYYKQFVRA
                     GAQLNDADQATLRALNQEESTLTTKFREHVLADTDASAIVVDTKAELAGLPDSDIAAA
                     ADRAKERKLEGKYVLTLQNTTQQPALTYLTNRAVRDRLFNASIQRGNHGGPNDTKAIV
                     TRLAQLRATKAKLLGFSTYAAYSLDDQMAKTPENAIKLLSDLVPASTAKARAEAAKMQ
                     KVIDAEKGGFQLGPQDWELYSEKVRKAEYDLDESQVRPYFELNNVLQNGVFFAANQLY
                     GLTFKERHDIPVYQPDVRVFEVFDADGKSIALYYGDYFARSNKSGGAWMDSFVDQSGL
                     IGTKPVVFNVTNFTKPAPGQPALLTFDNVTTIFHEFGHALHGMLSNVKYPTLAGTNVP
                     RDFVEFPSQFNEHWSLEPKVFANYAKHYQTGKPMPQALVDRIKKSRTFNQGYATVEYL
                     AAALLDMAWHTQAPGAPQADVNVFEPAALERFKIAMTTVPPRYHTTYFSHIWGGGYSA
                     GYYAYLWSELIDDDAYYWFKEHGGMTRANGQRFRDMILSRGGTMDEAALYRAFRGRDP
                     IVEPLLIERGLKEAPPAK"
     gene            4398..4835
                     /locus_tag="VNN25_07915"
     CDS             4398..4835
                     /locus_tag="VNN25_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91490.1"
                     /translation="MIEISTPAGLAYAQYTHKHEAPPRYGALIRVLPGIFESRPSTFA
                     SLVQERERFWVFFPVGPACHRGMVRVVAEEQLPLWAYRFPTFRSGRSGLWFLWDGETE
                     KQVEQLSEVERSLPWKNGIWNLAMLVTRIASGWMPPGLEKPDG"
     gene            complement(4985..5521)
                     /locus_tag="VNN25_07920"
     CDS             complement(4985..5521)
                     /locus_tag="VNN25_07920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HXH91491.1"
                     /translation="MKIIAICGSLRAHSSNRALLQAALKLAPEGMEVTIYEGLAGLPH
                     FNPDDDEEGTTPPPAVAELRSLLAAADGILISSPEYAHGVPGSLKNALDWLVSDGALV
                     DKPIALINASPVGGQFAQASLVETLTTMNWRVVGTWASPKKVRDENVDEEVAETIRAA
                     LVGLRDATQSRALQETQS"
     gene            complement(5717..7798)
                     /locus_tag="VNN25_07925"
     CDS             complement(5717..7798)
                     /locus_tag="VNN25_07925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXH91492.1"
                     /translation="MILPLALLADDPPAANPFFEKSTLPFQAPPFDKIKDSDFQPAIE
                     EGMKRELAEIQAIANNPDAPTFTNTIEAMEKTGDVLRRVQRVFGGLTQSNTNPTLQKI
                     QQAMAPKLAAHRDAINLDPKLFARIKAVYDARNKSGLDAEQKYLAERYYKNFIRSGAQ
                     LNEADKTALRAINQEQSKLTTQFRDRVLADTNAGAIVIDDKAMLDGLPESDISAAADR
                     AKAKGLDGKWLLTLQNTTQQPPQTYLKNRDLRKRLFEASIARGNHGGDNDTKAIITRL
                     AQLRAQRAKILGFSSYAAYNLEDEMSKTPENAFKLMTDMVPAATAKARGEAARMQKLI
                     DAQKGGFTLEPYDWQYYAEQVRKAEFDLDEGQVRPYFELDNVLQNGVFFAATKLYGIT
                     FKERHDIPVYQPDVRVFDVFDSDGKPLALFYADFFSRPNKAGGAWTSGFVGQSKLLGT
                     KPVVTNTENITKPSAGQPALLTFTEVTTMFHEFGHALHGMFSNVTYPSISGSSVPRDF
                     VEFPSQFNEHWAMEPTVFANYAKHYQTGAPMPKELVEKLKAARTFNQGFATTEYLGAA
                     LLDMAWHTIPADAPLLDVNAFEPESLKQYNIYMTQVPPRYRSTYFSHIWGGGYAAGYY
                     AYLWSEVLDDDAYYWFKEHGGMTRANGDKFRKMILSRGGTEDPAVMYRAFRGRDPKVE
                     PLLEERGLTGK"
     gene            8464..>10815
                     /locus_tag="VNN25_07930"
     CDS             8464..>10815
                     /locus_tag="VNN25_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91493.1"
                     /translation="MRMILRALVLTLLSTSALAAADPTLFSDLHWRLLGPFRAGRVCA
                     VTGVPGEGEHFYFGSVNGGVWETKDAGRTWQPIFDGQPIGSIGAIAVAPSNAKVIYVG
                     SGEADMRSDIAQGNGMYKSADGGKTWSHIGLTDSQQIGRIIVHPSNPDVVYVAALGHP
                     YAANAERGVFRSKDGGATWQRILSRNNDSDTGAIDLTFEPGNPNVIYASLWQTRRTPW
                     SVYPPSNGPGSGLFKSTDGGDHWTEIVGHGFPAKPGRIGIAVAPSQPQRVYAIVDAKE
                     GGMYRSDDGGANWTRASSDTRVWSRGWYFGGVTVEPKDADTVHSINVNVYTSTDGGKN
                     FVPSKGAPGGDDYHQLWIDPDHPERRILGVDQGTVISVNGGKTWSSWFNQPTGQFYHV
                     ITDNRFPYWVYGAQQDSGAAGIPSRTSTIDGISMVEFRETTAGGESDNIAPDPKDPEI
                     LYGSRVERLDTRTHQTQSVDPTLAYPGTDRRTWTLPLIFSPRDPHVLYFANQRMYRTD
                     DGGKHWTVISPDLTRENPETPATLDPVTAANKALPGPRQGVIYAIAPSRRIDHDIWAG
                     TDDGQIWRTHDEGAHWTNVTPAALTSWSKVGIIDASHFYGETAYVAVDRHRLDDFHPY
                     IYLTHDGGKSWQLIVNGIADDAAVNVVREDPVRRGMLYAGTERGIYVSRDDGAHWFPL
                     QLNLPITSVRDIDIHGDDVVIATHGRAFWVLDNVTALRQDVPAGDFLFAPAVTVRERP
                     AGFTGSPMPKDEPMAPNPPFGAYIDYAIRSAASQPVTIEILDAS"
BASE COUNT         1875 a         3269 c         3512 g         2159 t
ORIGIN      
        1 agttcgttgc gcttcgcgcg ttgtcgcttc gcgacttttc acgggatgtt cgcttagtgg
       61 cgcttcctcg tgacgttcgg caggctaagc tcgtgattct tgtccacagc tcgcttctag
      121 cccgcatttc tcacgcccct accgcgactc tttccaatcg agcgccttct ccccccgtgc
      181 ttttcggggg gagaaggtgc cgaaggcgga tgaggggggc aagatgggac aaacacgttg
      241 ttcgcgagcg tgaactgatt gacctggcca gtacctttgc aacgttgcag cgacagccag
      301 acgccccctc atccggcctt cggccacctt ctcccccact gaaaagcgtg ggggagaagg
      361 cgctcgattg ggagcgtggt gaaacaattt caacgggagt gtgagaaatg cgggctggac
      421 aacgcgcgaa gcgcaactaa ccgataaccg acaacgcggc gcccgccagg gcgccgcaat
      481 cattccgctg ccgcgatctg gtactgctcg atcttcccgt ccggcatgat gaacgtcgtc
      541 aggcggaggg tcttgttcgg gaacttgatc gtgtagcggc ggagggtcat gccgccgcgg
      601 agcgactgac cggcttgcga gaagtcctgt ggcgtgccga ggggagcgag gctcgaggcg
      661 aagtccttga tggcttgctc gtcgaagtag gcatttgcgt tcgacgtgaa ttgcgaacgg
      721 tcgatgcgtc ccttttgcag ttcgtcgaag atcttgcgca tctgttcggt ggtcttcgga
      781 gtggctggat cggttgatgc gaagatcgcg cgggagatgc cgccggcgat ctgctctgag
      841 gcgctggtgg cgtccatgtt ggtgaggacg acgatggcca cgcggtcatc gggatagacc
      901 tcgttgcgtg cggtaaagcc ggagacttcg ccgccgtgcg aaatgatgcg cttcccgttc
      961 gcgatgccga cgctgacgcc gaggccgtac cgcgatccca ctccattcgt catcggcacc
     1021 tcggcttcca gcacgcggta cgactccggc ttcaggaccg tctgatcgat catcgagatg
     1081 tcccacagcg ccagatcgtg cgcggtcatg gccagctcgc ccgcggcgaa catccagccg
     1141 cgtccttcct tcggcgcggg acgcaacggt cccagcgcgt agcggagata acgcatcgga
     1201 tcttcagggc cgaggggggc ggcgtcggtg ttcgagacgg tggtcatgtg gagagggacg
     1261 aagacgcgct tctgcagaaa gtcgagcagc ggctctccgg ccaccttctc cgcgatcatc
     1321 cctgcgatga cgtagttcgt gttgctgtac tgccatttcg cgcccggctc gaaatcgagc
     1381 ggcttcttcg cccagccggc tacgatctcg ggtgcggtga ccggctgcat catcatcggc
     1441 atgacgtagt cctgcggcca gaaatcctgg tagccggacg tcatcgaaag aatctggcgg
     1501 atggtgacgt cgccggcgcg cgtgaggtcg ggcagccagc ggatgacctt gtcgtccagc
     1561 gagagcttgc cttcttcggc gagcaggagc agcgcagtgg ccgtgaactg cttgctgatc
     1621 gagccgatcg agtagcgcat cttcggcgac gccgccagct tcgtctcgag attggccgtg
     1681 ccgtacgcgt tcgtgtagac gatcttgccg tcgcggacga ccgcgatcga agcactcggc
     1741 gcgccggtct tggcgaggat gtcgtttgcc gatttgtcga tgctggcccg catgtcggcg
     1801 gggagctctt gggcacttag gacggcggtg aaggcgagtg cgaggagggc ggtgacgatt
     1861 ctgcgcatgc gcgcgattct acgacgggag tcgggagtcg ggtgtcgggt gtcgggtgtc
     1921 ggagcagtgg aagacttttc tccgaccccc gacccccgac ccccgcaaga ctacctaccc
     1981 ggcgccacga cagagtcggg tctcggagcc gtagaagatt tctctccgac ccccgacccc
     2041 cgaccttcgc aaatctactt cgctggcggt gcttccttca gccctctctc aatcaacagc
     2101 ggctccacga tcggatcgcg gccgcggaag gcgcggtaca gcgcggcttc atccatcgtg
     2161 ccgccgcgcg acagaatcat gtcgcggaag cgttggccgt tggcgcgcgt catcccgccg
     2221 tgctccttga accagtagta cgcgtcgtcg tcgatcaact cgctccagag gtacgcgtag
     2281 tagccggccg agtagccgcc gccccagatg tgcgagaagt aggtcgtgtg atagcgcggc
     2341 ggcaccgtgg tcatggcgat cttgaaacgc tcgagcgctg ccggctcgaa gacgtttacg
     2401 tccgcctgcg gcgcgccggg cgcctgcgtg tgccaggcca tgtcgagcag cgcggcggcc
     2461 aggtactcga ccgtcgcgta gccctggttg aacgtgcgcg acttcttgat acggtcgacc
     2521 agggcctgcg gcatcggttt gccggtctgg tagtgcttcg cataattcgc gaagaccttc
     2581 ggctccagcg accaatgctc gttgaactgc gacgggaact cgacgaagtc gcgcggcacg
     2641 ttcgtgccgg ccagcgtcgg gtacttcacg ttcgacagca tgccgtgcag cgcgtggccg
     2701 aactcgtgga agatcgtggt gacgttgtcg aacgtcagca gcgccggctg gccgggcgcg
     2761 ggcttggtga agttcgtgac gttgaagacg actggcttgg tgccgatgag tcccgactga
     2821 tcgacgaagc tgtccatcca ggcgccgccg ctcttgttcg agcgcgcgaa gtaatcgccg
     2881 tagtagagcg cgatcgactt gccgtcggcg tcgaagacct cgaacacgcg cacgtccggc
     2941 tgatagaccg ggatgtcgtg ccgctccttg aacgtcagtc cgtagagctg attcgcggcg
     3001 aagaagacgc cgttctgcag aacattgttc agctcgaagt acggacgcac ctgcgactca
     3061 tcgaggtcgt actccgcctt gcgcaccttc tcggagtaga gctcccaatc ctgcgggcca
     3121 agctggaagc cgcccttctc cgcgtcgatg actttctgca tcttcgccgc ttcggcgcgc
     3181 gccttcgccg tcgatgccgg aacgagatcg ctcagcagct tgatggcatt ctcgggcgtc
     3241 ttggccatct gatcgtcgag cgaataggcg gcataggtcg aaaatccaag gagcttggct
     3301 ttcgtggcgc ggagctgggc gaggcgggta acgatcgctt tcgtgtcgtt cggtccgccg
     3361 tgattgccgc gctgaatcga cgcgttgaac agccggtcgc ggacggcgcg gttggtgaga
     3421 taggtcagcg ccggctgctg cgtcgtgttc tgcagcgtga ggacgtactt cccttcgagc
     3481 ttgcgctctt tggcgcggtc cgcggcggcg gcgatgtcgg aatcgggcag accggccagc
     3541 tctgccttcg tgtcgacgac gatggccgag gcgtcggtgt cggcgagaac gtgctcgcgg
     3601 aacttcgtcg tcagcgtcga ctcttcctga ttgagtgcac gcaacgtggc ctgatcggcg
     3661 tcattgagct gcgcaccggc gcggacgaac tgcttgtagt aacgctcgat gaggaacttc
     3721 gactcggcgt cgagcccgct cgcagcgcgg ctgtcataca aagtcttgac ccgcgcgtag
     3781 agcttgggat tcatgtagat cgcatccccg tgcgccgcca gcttcggcga ctcctcggcc
     3841 tcgatcttct ggaacgtttc gttcgtattc gactgcgtga ggttgaagaa gatcttggac
     3901 gcgcgcgtga gcagcgcccc cgacctctcc atcgcctcga tcgtgttggc gaaggtcggc
     3961 ggcgccgggt cgttggcgat ggcgtcgatc tcccggagct gaaccttcat cccttcctcg
     4021 atggcgggct gatagtcggt gtccttgatc tgatcgaacg gcggcgcctg atacagcagc
     4081 gggctggcgg tggagagagg gttctgacgt gccggttccg cggcggtttg cgcctccagg
     4141 acgcatgcgc tgatcatgaa tacggccccc agaagtgtcg aaatgcttgt tttcatgacg
     4201 gtggggaaca agggaagagg gagatgtcat cccgtagagt gagggtggtt ccttcttgca
     4261 tccgtgcgct ggctactgat cgttcgcagg aagtagtgtg cgatgatcgg ggaacgcata
     4321 attgatcgcg gcaagactgc gttggcgtcc ctatctccat gccactgacc gcacgcaagc
     4381 gtccgaggcc gggcgacgtg attgagatct ccacgccggc gggccttgcc tatgctcagt
     4441 acacgcacaa acatgaagcg ccgcctcgat acggtgcgct gattcgggtt cttcccggca
     4501 tcttcgaatc ccgcccatct acgttcgctt ctttggtcca ggagcgcgag cgcttctggg
     4561 tgttctttcc ggttggacct gcttgtcatc gcggaatggt tcgtgtggta gccgaggagc
     4621 aattaccact ttgggcttac cgcttcccta cgtttcggtc tgggcggagt ggactctggt
     4681 ttctatggga cggcgagact gagaagcagg tcgaacaact cagtgaggtg gagcggagcc
     4741 tgccttggaa aaacggcatt tggaacctgg ccatgctggt cacccgtatc gcgagtggct
     4801 ggatgccgcc gggcttagag aaaccggacg gctagcggtt tgcgcagcag aaactatgag
     4861 tcgttcgacc tccgacctga cagcactcgg gcgaggacgc ccaagtgcgg ccatcgaggc
     4921 gtcccgcctc ggtggttgga tccagcccga tagtccagtc ggttgaaacg gatccaaaac
     4981 aaacctagct ttgggtctct tggagagcac gcgattgcgt tgcatcgcgc aatcccacca
     5041 gcgccgcgcg aatcgtctcg gctacttcct catcgacgtt ctcatcgcga accttcttcg
     5101 gcgacgccca cgtgcccact acgcgccagt tcatcgtcgt caacgtctcg acgagcgagg
     5161 cttgggcgaa ttggccgccg actggcgatg cgttgatcag ggcgatcggt ttgtcgacga
     5221 gagcgccgtc ggagacgagc caatcgaggg catttttcag tgagccgggg acgccgtgcg
     5281 cgtactcggg agatgagatc aggatgccgt ctgcggcggc gagaagtgat cgaagttcgg
     5341 cgacggcggg cggtggagtg gtgccttctt catcgtcgtc gggattgaag tgggggagcc
     5401 cggccagacc ttcgtagatc gtgacctcca tcccttccgg agcgagcttc aaagccgcct
     5461 gcagcagggc gcggttcgag gagtgcgcgc ggaggctgcc gcagatggcg atgattttca
     5521 ttccgttctt tccgctgtcg tcgatgaggg tttacgtagg tcggactctc tagtccgaca
     5581 tcacgtgcag tcggactgga gagtccgacc tacgtaaacc acggacatca cgtgcagtcg
     5641 gactggagag tccgacctac gtaaactacg gtgcagtcgg accgacctag gtaaaccacg
     5701 gtctcgcgat ctaggcctac ttccccgtca atccgcgctc ttccagcaac ggctcaacct
     5761 tcggatcgcg tccgcggaac gcccgataca tcacggcagg atcttcggtg ccgccgcggg
     5821 agaggatcat tttgcggaac ttgtcgccgt ttgctctcgt catgccgccg tgttccttga
     5881 accagtagta ggcatcgtcg tcgagcactt cgctccacag atacgcgtag tagccggctg
     5941 cgtagccgcc gccccagatg tgggaaaagt acgtcgagcg gtagcgcggc gggacctgcg
     6001 tcatgtagat gttgtactgc ttgagcgact ctggctcgaa ggcattcaca tctagtagcg
     6061 gcgcgtcggc ggggatggtg tgccacgcca tgtcgagaag ggcagcgccg aggtattcgg
     6121 tggtggcgaa accctggttg aaggtgcgag cggccttgag cttctcgacc aactccttcg
     6181 gcatcggtgc gccggtctgg tagtgcttgg cgtaattggc gaagaccgta ggctccatcg
     6241 cccaatgctc gttgaattgc gacgggaact cgacgaagtc gcgcggcacg gacgatccgg
     6301 agatcgacgg atacgtcacg ttcgagaaca tgccgtgcag ggcgtggccg aactcgtgaa
     6361 acatcgtcgt cacttcggtg aaggtgagga gcgctggctg acccgctgaa ggcttggtga
     6421 tgttctccgt gttcgtgacc acgggcttcg tgccgaggag tttgctctgg ccgacgaaac
     6481 cgctggtcca ggcgccgccg gctttgttcg gacgcgagaa gaagtcggcg tagaagagcg
     6541 ccagcggctt gccgtcgctg tcgaagacat cgaagacgcg cacgtcgggt tgatagacgg
     6601 ggatgtcgtg ccgctctttg aacgtgatgc cgtagagctt cgtagccgcg aaaaagacgc
     6661 cgttctgcag tacgttgtcg agctcgaagt atggccggac ctgcccttca tcgagatcga
     6721 attccgcttt gcgaacctgc tcggcgtagt actgccagtc gtacggctcg agcgtgaagc
     6781 cgcctttctg tgcgtcgatg agcttctgca tgcgggccgc ctcgccgcgc gctttcgccg
     6841 tcgccgccgg gaccatgtcg gtcatcagct tgaaggcgtt ctcgggcgtt ttcgacatct
     6901 cgtcctcgag gttgtaggcg gcgtaactcg agaatcccag gatcttggcg cgttgcgcgc
     6961 gaagctgggc caggcgggtg atgatcgcct tcgtgtcgtt gtcgccgccg tgattaccgc
     7021 gcgcgatcga cgcttcgaag agccgcttgc gaaggtcgcg attcttgagg tacgtctgcg
     7081 gcggctgctg cgtcgtgttc tgcagcgtga gcagccattt gccgtcgaga ccttttgcct
     7141 tggcgcggtc ggcggctgcg gagatgtcgg attccgggag gccgtcgagc atggccttgt
     7201 cgtcgatgac gatggcgccg gcgttcgtgt cggcgagcac gcggtcgcgg aactgcgtgg
     7261 tcagcttcga ctgctcctga ttgatggcgc ggagcgcggt cttgtcggct tcgttgagct
     7321 gcgcgccgga gcggatgaag ttcttgtagt agcgctcggc caggtacttc tgctcggcgt
     7381 cgagccccga tttgttgcgg gcgtcgtaga ccgctttgat gcgcgcgaag agcttcggat
     7441 cgagattgat ggcgtcgcgg tgcgcggcca gcttcggcgc catggcctgc tgaatcttct
     7501 gcaacgtcgg attcgtgttc gattgcgtca ggccgccgaa gacgcgttgc acgcggcgga
     7561 gcacgtcgcc ggtcttctcc atcgcctcga tcgtgttggt gaaggtcggg gcatcgggat
     7621 tgttggcgat ggcctggatc tcggccagct cgcgtttcat cccttcctcg atggcaggct
     7681 ggaagtcgct gtccttgatt ttgtcgaacg gcggcgcctg gaaggggagc gtgctctttt
     7741 cgaagaacgg attcgcagcc ggcggatcgt cggcgaggag agcgagcgga aggatcatgg
     7801 cgatggcgat gagcgtgagc agcaatttca tggggagcgt tatacacatt cagttgttac
     7861 cgcgctgtga cgggtgatgg ttttttcggg gacgccgtga tcgccaggca aaggcaacca
     7921 cagagacaca gagaacacag aggagtctct gtgacctctg tgcctctgtg gtaaccgggt
     7981 tttcaaagtg acccaatacg cgattgcgtg gatggcatcg agcgatacca tcgatggcat
     8041 caccccatgg catcgatggt gtcgactcat ggcatcgatg gcatccaccc atgccatcga
     8101 tggtatcgac ccatgccatc gatggcattg acccatggca tcgatggtat cgacctatgc
     8161 catcgatggt atcggcccat accatcgatg gcatcacccc atggcattga tggtgtcgac
     8221 tcatggcatc gatggcatcc acccatggca tcgatggcat cgacccatgc catcgatggt
     8281 attgacccat gccatcgatg gcatcgaagg agccatccat aatgaccaaa ccaggcatcg
     8341 atggctcgag tctctccgcc gatgcctacc gccttccact cccaccccac ctgtcgtcac
     8401 agagcggcga aatgtccaca gcaagccgga ggacctccca aaccgcggct atcattcgcg
     8461 cccatgcgaa tgatcttgcg cgcgctcgtc ctcacgcttc tctcgaccag tgctctcgcc
     8521 gcggccgacc cgaccctctt cagtgacctc cactggcgtc tcctcgggcc cttccgcgcg
     8581 ggacgcgtct gcgccgtcac cggcgtgccg ggtgaggggg agcatttcta cttcggcagc
     8641 gtgaacggcg gggtgtggga aacgaaggat gccgggcgca cgtggcagcc gattttcgac
     8701 ggacagccga tcggctcgat cggcgcgatt gccgtggcgc cgtcgaatgc gaaggtgatc
     8761 tacgttggca gcggtgaggc tgacatgcgc tccgacatcg cccagggcaa cggcatgtac
     8821 aagtcggccg acggcggcaa gacgtggtcg cacatcggac tgaccgactc ccagcagatc
     8881 ggacggatca tcgttcaccc atccaacccc gacgtcgtct acgtcgcggc cctcgggcat
     8941 ccctacgccg ccaacgcaga acgtggcgta tttcgctcga aggatggcgg cgccacgtgg
     9001 caacgcattc tctcgcgcaa caacgacagc gacacgggcg cgatcgacct caccttcgag
     9061 ccgggcaatc cgaatgtgat ctatgcgtcg ctctggcaga cgcggcgcac gccgtggagc
     9121 gtctatccgc cgtcgaacgg gcccggaagc ggtctgttca aatcgaccga cggaggcgat
     9181 cactggacgg agatcgtcgg acatgggttc ccggccaagc ccggccgcat cggcatcgcc
     9241 gtcgccccgt cgcagccgca gcgcgtctac gccatcgtcg acgcgaagga aggcggcatg
     9301 taccgcagcg acgacggcgg cgcgaactgg acgcgcgcga gcagcgacac gcgcgtctgg
     9361 tcgcgcggct ggtacttcgg cggcgtgact gtcgagccga aagacgccga caccgtgcac
     9421 tcgattaacg tcaatgtcta cacctcgacg gacggcggca agaacttcgt gcccagcaaa
     9481 ggcgcgcccg gcggcgacga ctatcaccag ctctggatcg atcccgatca tccggagcgc
     9541 cgcatcctgg gcgtcgatca gggcaccgtc atctcggtca acggcgggaa gacgtggtcg
     9601 agctggttca atcagccaac cggccagttc taccatgtca tcaccgacaa ccgctttccg
     9661 tactgggtct acggcgcgca gcaggattcg ggcgcggcgg gtattcccag tcgcacgagc
     9721 acgatcgacg gcatttcgat ggtggagttt cgcgagacca ccgcgggcgg cgagagcgac
     9781 aacatcgcgc ccgatccgaa agaccctgag attctgtacg gaagccgcgt ggagcggctc
     9841 gatacgcgca cgcaccagac gcagtcggtc gatccgacgc ttgcgtatcc cggcaccgat
     9901 cgccgcacgt ggacgctgcc gctgattttc tcgccgcgcg atccacacgt tctctacttc
     9961 gccaaccaga ggatgtaccg caccgatgat ggcgggaaac actggacggt catcagcccc
    10021 gatctgacgc gcgagaatcc ggagacgccg gccactctcg atccggtgac agcggccaac
    10081 aaggcgcttc ccggcccgcg gcaaggcgtg atctacgcga tcgcgccgtc gcgcaggatc
    10141 gatcacgaca tctgggccgg caccgatgac ggccagatct ggcgcacgca cgacgaagga
    10201 gcgcactgga cgaacgtgac gccggccgcg ctgacgtcgt ggtcgaaggt cggcatcatc
    10261 gacgcttcgc atttctacgg cgagacagcc tacgtcgcgg tggaccgtca tcgcctcgac
    10321 gactttcacc cgtacatcta tctcacgcat gacggcggca agtcgtggca gctcatcgtc
    10381 aacggaatcg cggacgatgc cgcggtgaat gtggttcgcg aggatccggt gcgccgcggc
    10441 atgctctacg cgggcacgga gcgcgggatc tacgtgtcgc gcgatgacgg cgctcactgg
    10501 ttcccgctcc aactgaatct ccccatcacc tcggtgcgcg atatcgacat ccacggcgac
    10561 gatgtcgtca tcgccacgca tggccgcgcc ttctgggtgc tcgacaacgt gacggcgctg
    10621 cggcaggacg tgccggccgg cgatttcctt tttgcgcccg cggtcaccgt acgtgaacgg
    10681 cctgccggat tcaccggctc gccaatgccc aaagatgaac cgatggctcc gaatccgccg
    10741 ttcggcgcgt acatcgacta tgcgatccgc agcgccgcat cgcagccggt gacgatcgag
    10801 attctcgacg cctcc
//
LOCUS       DATLVW010000248         3035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_298848, whole genome shotgun sequence.
ACCESSION   DATLVW010000248 DATLVW010000000
VERSION     DATLVW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298848"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /gene="kdpA"
                     /locus_tag="VNN25_07935"
     CDS             <1..1204
                     /gene="kdpA"
                     /locus_tag="VNN25_07935"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXH91494.1"
                     /translation="FWVDLTRTTLWLLLPICLASAVVLVSQGVIQNFHPTTAITTVEG
                     KIQKIPQGPVASQEAIKELGTNGGGFFNANSAHPYENPTPFTNLFEMFLIFAIGAGLT
                     YTLGSMIGKQRHGWAVFWAMGIVFLAGTFVVTIAEQHGNPLHQRLGVASADGNMEGKE
                     VRFGIGDSSLFAAITTDASCGAINSWHDSFTPIGGSIPLLNIMLGEVIFGGVGAGLYG
                     MFLFIVLTVFIAGLMVGRTPEYMGKKIEAHDVKLAMLAVLVLAFSILVGAAAAAVTTA
                     GTSSLANAGPHGLSEMTYAFTSATGNNGSAFAGLNANTKFYNIALSFAMFIGRFLMII
                     PIMGIAGSLAAKKKLAETVGTFPVDGPLFTFLLIGVIVIVGALTYFPVLALGPYVEHF
                     LMNAGKVF"
     gene            1208..>3035
                     /gene="kdpB"
                     /locus_tag="VNN25_07940"
     CDS             1208..>3035
                     /gene="kdpB"
                     /locus_tag="VNN25_07940"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HXH91495.1"
                     /translation="MATQTKLSLFDGAILRRALTDSIVKLSPRRMMKNPVMFLVEVGA
                     VLTTILLIPGLSKHVDYLFQIQITLWLWATVLFANLAEAVAEGRGKAQADALRKAKTD
                     AVARKLTGSGPASSRREERVAASALRKGDLVVVETNQTIPGDGTVIEGIASVNESAIT
                     GESAPVIREAGGDRSAVTGGTTVLSDRIVVEITSDPGQTFLDRMIKLVEGAERQKTPN
                     EVALDILLAGLTIIFLFATITLKPFALYSSSDVSVTVLIALLVCLIPTTIGALLSAIG
                     IAGMDRVLQHNILAMSGRAVEASGDVNVLLLDKTGTITLGNRQATAFVPAPGVDAATL
                     AEIAQLSSLADETPEGRSIVILAKEQFGLRGRELRELGAHFVPFTAKTRMSGLDFEGG
                     RAIRKGAADSVAAFVKTFDGVFPEQVEKDVARIAGEGGTPLVVAENGRVLGTIYLKDI
                     VKGGMMERFDRLRMMGIRTVMITGDNPLTAATIAREAGVDDFLAQATPEDKLALIRKE
                     QAKGNLVAMTGDGTNDAPALAQADVGMAMNTGTQAAKEAGNMVDLDSNPTKLIEVVEI
                     GKQLLMTRGALTTFSISNDVAKYFAIIPAMFVATYPALQTLNV"
BASE COUNT          559 a         1000 c          928 g          548 t
ORIGIN      
        1 tttctgggtt gacctcacgc gaactactct ctggctgcta ctgccgatct gtttggcgtc
       61 ggcggtggtg ctcgtgtcgc agggagtgat ccagaacttc catccgacga cggcgatcac
      121 gacggtcgag ggaaagattc agaagatccc gcaaggtcct gtggcctcgc aggaggcgat
      181 caaagagctt ggcaccaacg gcggcggttt cttcaacgcc aactcggcgc atccgtacga
      241 gaaccccacg ccgttcacca acctttttga gatgttcctc atcttcgcca tcggggccgg
      301 gctgacgtac acgctcggct cgatgatcgg caagcagcgt cacggctggg cggtcttctg
      361 ggcgatgggg atcgtctttc tggccggcac gttcgtcgtc acgatcgcgg aacagcacgg
      421 caatcctctc catcaacgcc tcggcgtcgc cagcgccgac ggcaacatgg aagggaagga
      481 ggtccgcttc ggaatcggcg actcgtcgct gttcgctgcc atcaccaccg acgcctcgtg
      541 cggcgcgatc aactcgtggc acgactcctt cacgcccatc ggcggctcca tcccgcttct
      601 caacatcatg ctcggcgagg tgatcttcgg cggtgtcggc gcaggcctct acggcatgtt
      661 cctcttcatc gtgctgacgg tcttcatcgc cggactgatg gtcgggcgga cgccggagta
      721 catgggcaag aagatcgagg cgcacgacgt gaagctggcc atgctggccg tcctggttct
      781 ggcgttctcg attctcgtcg gcgccgccgc ggccgcggtc acgacagccg gcacgtcgag
      841 cctcgccaac gccggcccgc acggtctcag cgagatgacc tacgcgttca cgagcgccac
      901 cggcaacaac ggcagcgcct tcgccggtct caacgccaac acgaagttct acaacatcgc
      961 actcagcttc gccatgttca tcggccgctt cctgatgatc atcccaatca tgggcatcgc
     1021 cggctcgctc gccgcaaaaa agaagctcgc cgaaacggtg ggcacgttcc cggttgacgg
     1081 accgctgttc acgttcctgc tcatcggggt gatcgtcatc gtcggcgcgc tcacgtactt
     1141 cccggtcctc gccctcgggc cgtacgtcga gcacttcctc atgaacgcag gaaaggtgtt
     1201 ctaagccatg gccacgcaaa cgaaactctc actgtttgac ggggccatcc tccgccgcgc
     1261 gcttacggac tcgatcgtca agctgagtcc gcgccggatg atgaagaacc cggtcatgtt
     1321 cctcgtcgaa gtgggcgcgg tgctcacaac gatccttctc atccccggcc tctcgaagca
     1381 cgtcgactat ctctttcaga tccagatcac actctggctc tgggcgacgg tgctcttcgc
     1441 caacctcgcc gaagcagtag ccgagggacg cggaaaggcg caggcggatg cgctgcgaaa
     1501 ggcgaagacc gatgcggtcg ctcgcaagct gacgggcagc ggcccagcat cgtcgcgccg
     1561 cgaagagcga gtcgctgcct cggcgctgcg caagggcgat ctggtcgtcg tcgaaacgaa
     1621 ccagaccatt cccggcgatg gcaccgtcat cgaaggcatc gcctcggtca acgaatcggc
     1681 gattaccggc gagtccgcac ccgtcatccg cgaagcaggc ggcgaccgct ccgctgttac
     1741 cggcggcacg acggtcctct ccgaccgcat cgtcgtcgag atcacctcgg atcccggcca
     1801 gacgttcctc gaccgcatga tcaaactggt agaaggtgcg gaacggcaga agacgccgaa
     1861 cgaagtcgcg ctcgacatcc tgctggccgg gctgacgatc atcttcctct tcgccaccat
     1921 cacgctcaaa ccgttcgcgc tctattcatc gagcgacgtg tccgtgacgg tgttgatcgc
     1981 gctgctcgtc tgcctcattc cgacgacgat cggcgcgctt ctctcggcga tcggcatcgc
     2041 cggcatggac cgcgtactgc agcacaacat cctggcgatg tcgggccgcg cggtcgaagc
     2101 gtcgggcgat gtcaacgtgt tgctgctgga caagaccggc acgatcacgc tcggcaatcg
     2161 tcaggcgacc gcgttcgtcc ccgctcccgg cgtcgacgcc gcgacgcttg ccgagatcgc
     2221 gcagctatcg tcgctcgctg atgagactcc cgagggacgg tcgattgtga ttctggcgaa
     2281 agagcagttc ggattgcgcg gccgcgaatt gcgcgagctc ggcgcgcact tcgtgccgtt
     2341 caccgcgaag acgcgcatgt ccggactcga cttcgaagga gggcgcgcca tccgcaaggg
     2401 agctgcagat tccgttgcgg cattcgttaa gacgttcgac ggcgtgtttc ccgagcaggt
     2461 ggagaaggac gtcgcccgga tcgccggcga aggcggcaca ccgctcgtcg tcgccgagaa
     2521 cggccgcgtg ctgggaacga tctatctcaa agacatcgtc aagggcggga tgatggagcg
     2581 cttcgaccgc ttgcgcatga tgggcattcg caccgtcatg atcaccggcg acaacccgct
     2641 gaccgcggcc acgattgcgc gcgaggccgg cgtcgatgac tttctcgcgc aggcgacacc
     2701 cgaggacaag ctcgcgctga ttcggaaaga gcaggccaag gggaacctcg tggcgatgac
     2761 cggcgacggc acgaacgacg cccccgctct ggcgcaggcc gacgtcggta tggccatgaa
     2821 cacgggcacg caggcagcga aagaggccgg caacatggtc gacctcgatt cgaatccgac
     2881 caagctgatc gaagtcgtcg agatcggcaa acagcttctg atgacgcgcg gcgcgctgac
     2941 gacgttctcg atctccaacg acgtcgcgaa gtacttcgcg atcattccgg cgatgttcgt
     3001 cgcgacctat ccggcgctgc aaacgctcaa tgtca
//
LOCUS       DATLVW010000249         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_216812, whole genome shotgun sequence.
ACCESSION   DATLVW010000249 DATLVW010000000
VERSION     DATLVW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216812"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1575)
                     /gene="purF"
                     /locus_tag="VNN25_07945"
     CDS             complement(85..1575)
                     /gene="purF"
                     /locus_tag="VNN25_07945"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXH91496.1"
                     /translation="MFDKFHDECGVFGVLNHENAANIAYLGLYALQHRGQESAGIASV
                     SSDRRRSETNIGAASGASSGSGEQIIAAGAPQIHSEKEMGYVAEIFTEERLRRLPGDI
                     AIGHVRYSTAGGSMLCNAQPLVASTSKGPIALAHNGNLVNGADIRRRLEADGAIFNTM
                     SDTEVIVHLIARSKEADLEHALVDALSQVKGAYSIVLLAPGRIYAARDSYGFRPLVLG
                     RLGESVVVSSETCAFDLIEAVTIREVRAGEVVVIECAEPSPRIRVVHEFTGPREARCI
                     FEHVYFARPDSVIFGNNVAEVRKRFGAQLAREHAIAADVVVPVPDSGVFAALGYAHES
                     GIPFEFALVRNHYVGRTFIEPKQSIRSFGVKVKLNPVREIVEGKRIVLIDDSIVRGTT
                     SKKIVRMLKDFGAKEVHMRISSPPTTGPCYYGIDTPQRRDLIASAQSVESIREFIEAD
                     SLGYLSEEGMLAAVRNDDDPRRLYCTACFTGKYPVLHEEAEAAAFV"
     gene            complement(1613..2512)
                     /locus_tag="VNN25_07950"
     CDS             complement(1613..2512)
                     /locus_tag="VNN25_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH91497.1"
                     /translation="MTTTLTHPTAEDLGCFIEGTLDDQARAAIVEHLADCDDCRITVV
                     DAVEFAEPAAFDEKVAVKQSAAGGLWWMTAAASIAIVVGGVWLVQAWRDPLAPLKEAS
                     SRLSSRLVDARLSDFPYVVRKSRMRGSSGDTDLAAPQVALKADEVLNRAGTDARMQHA
                     KGVALLLRVEAELPEHTENNADDNGARLELVKQRNEAVAMLQVAADREPDNAAFQSDL
                     AGALMATGDPKNINLAADICSRVVKRNPQSFEALFNQAKALDLAGRTPEAIATYKLYV
                     KVDPASPWSTEANERLKALSEEL"
     gene            complement(2509..3324)
                     /locus_tag="VNN25_07955"
     CDS             complement(2509..3324)
                     /locus_tag="VNN25_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH91498.1"
                     /translation="MDWERFFLDNLPLIDSATAFVCRKYGLIGADAEDFRSVIMIKLI
                     EDDYAVLRKFENKCALKTYLVIVIKRHYADLKIHEWGKWRPSVRARQGGDAAILMERL
                     MSRDGKGQAEASAIVRQKYTELDSRALEAIASSIVTRRAQRAEEVERTEEMPEPASGI
                     SAEDDLLTNERKAAECRTSAIVNRELGLFPAEDRLVMKLRYLDGMKVSAMARMLKVDQ
                     KRLYRRIERLGARLKEALLGAGIAIRDIRDMLTNGADALQFDLGPDDRPKALQ"
     gene            complement(3421..>3602)
                     /locus_tag="VNN25_07960"
     CDS             complement(3421..>3602)
                     /locus_tag="VNN25_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91499.1"
                     /translation="GWTGHRGLKNGVTVEIRAMDAQLAAALIACQEDPASLPFVTLDL
                     DLAAAARAEGFAVLP"
BASE COUNT          661 a         1147 c         1104 g          690 t
ORIGIN      
        1 ggccacgctg cccgggcgtc tgatcggaca tccgctcgat gctcccgcct ttcggatgta
       61 tgtgtagggc gagcgttcgc gcgtttagac aaacgccgcg gcttctgcct cttcgtgtaa
      121 caccggatac ttccctgtga agcacgcggt gcagtaaagc cgccgcggat cgtcgtcatt
      181 acgcacggcc gcgagcatgc cttcttcgga gaggtagccg agcgaatcgg cctcgatgaa
      241 ttctcggatc gattcgaccg attgggcgct ggcgatgagg tcgcgccgct gcggtgtgtc
      301 gattccgtag tagcaggggc cagtcgtagg cggcgaagag atgcgcatgt gcacctcttt
      361 ggcgccgaag tccttcagca tccgcacgat cttcttcgac gtggtaccgc ggacgatcga
      421 gtcgtcgatc agaacgatgc gcttgccttc gacgatctca cgcaccgggt tgagcttcac
      481 cttcacgccg aagctgcgga tggattgctt cggctcgatg aaggttcgtc cgacgtaatg
      541 gttgcgcacg agcgcgaact cgaacgggat gcccgactcg tgcgcgtagc cgagcgcagc
      601 gaagacgccg gagtcaggaa cgggaacgac gacgtcggcg gcaatggcgt gctcgcgcgc
      661 cagttgcgcg ccgaaccgct tccgcacctc ggccacgtta ttgccgaaga tcacagaatc
      721 aggccgggcg aagtagacgt gctcgaaaat gcagcgcgcc tcgcgcggtc ccgtgaattc
      781 gtggacgacg cggatgcgtg gtgacggttc ggcgcactcg atcacgacga cctcgccggc
      841 gcgcacttcg cggatcgtca cggcttcgat gaggtcgaac gcgcacgttt cggaggagac
      901 aacaaccgat tcgccgaggc gcccaaggac gagaggccgg aatccgtacg aatcgcgcgc
      961 cgcgtagatg cggccgggag cgagcagcac gatcgaatac gcgcctttga cctgggagag
     1021 cgcatcgacg agggcatgct caagatcagc ctcctttgac cgcgcgatca gatgcacgat
     1081 gacctcggtg tccgacatcg tgttgaagat cgccccatca gcttcgagac gccgccggat
     1141 atcggcgccg ttcaccaggt tgccgttgtg cgccagcgcg atcggcccct tactggtcga
     1201 agccacgagc ggctgcgcgt tgcaaagcat cgatccgccg gcagtggagt agcgcacatg
     1261 cccgatggca atgtccccgg gaagcctccg cagccgctcc tcggtgaaga tctcggcgac
     1321 gtagcccatc tccttctcgg agtgaatctg cggcgctccc gccgcgatga tctgctcccc
     1381 gctcccgctc gaagccccac tggccgcccc gatattcgtc tccgaccgcc gccggtccga
     1441 cgacaccgaa gcaatcccgg cactctcctg cccccgatgc tgcagcgcat acagcccgag
     1501 gtaagcaatg ttcgccgcgt tctcatgatt cagaacgcca aacacgccgc attcatcgtg
     1561 gaacttgtcg aacatgggga ccgccggagg aagtgtagcg caggggggaa aatcagagct
     1621 cttcgctcaa ggccttgagt cgttcgttcg cctcagtgga ccagggtgac gcggggtcga
     1681 ccttgacgta gagcttgtac gtggcaattg cctcaggcgt ccgaccggcc aaatccagcg
     1741 ctttcgcttg attgaaaagt gcctcgaagg actgtgggtt acgcttcacg acgcgactgc
     1801 aaatgtcggc ggccagattg atgtttttcg ggtctcccgt ggccatgagt gcgccggcga
     1861 gatcactctg gaaggcggcg ttgtctggct cacgatcagc agccacttga agcatcgcga
     1921 ctgcctcgtt ccgctgcttg acgagctcga gccttgcgcc gttatcgtcg gcgttgtttt
     1981 cggtgtgctc agggagttcg gcttctaccc tcaaaagaag cgcgacgccc tttgcgtgct
     2041 gcattctggc gtcagttcca gcgcgattca ggacctcatc ggccttcaat gcaacctgcg
     2101 gggcggcaag atccgtatcg ccgctcgagc cgcgcattct gctcttccgc acgacgtacg
     2161 gaaagtcgct cagtcgtgcg tccaccagac gcgagctcaa gcgcgaagat gcttccttca
     2221 acggcgccag cggatcacgc cacgcctgga caagccacac acctccgacg acaatcgcga
     2281 ttgaagcggc tgccgtcatc caccacagcc cacccgcagc gctttgcttg actgccacct
     2341 tctcgtcaaa ggccgcgggc tccgcaaact ccacggcatc caccacggtg atccggcagt
     2401 catcgcagtc ggcaaggtgc tcgacgatcg cggcgcgcgc ttgatcgtcg agggtgccct
     2461 cgatgaagca gccgaggtcc tcggccgtgg ggtgggtgag ggtcgtggtc attggagtgc
     2521 cttcggacga tcgtcgggac caagatcgaa ctggagcgca tcggcgccat tggtgagcat
     2581 gtctcgaatg tcgcgaattg cgatgccggc gccaaggagc gcttccttca gcctggcgcc
     2641 gaggcgctcg atccggcggt agagccgttt ctggtcgacc ttcagcatcc tggccatggc
     2701 cgacactttc attccatcaa ggtagcgaag tttcataacc aatcggtcct cggccggaaa
     2761 gagtccgagc tcccgattca cgatcgcact ggtgcggcac tcggccgctt tgcgctcgtt
     2821 cgtcagaagg tcgtcctcgg ccgatatgcc ggatgcgggc tcgggcattt cctccgtccg
     2881 ctcgacttcc tccgcccgtt gcgcgcgacg cgtcacgata gacgaggcga tcgcttccag
     2941 cgcacgggag tcaagctccg tgtatttttg acggacaatt gctgaagctt ccgcctgacc
     3001 cttcccatcg cggctcatca ggcgctccat gagaatggcc gcatcgcctc cttgccgcgc
     3061 ccgcaccgat ggccgccatt tgccccattc gtgaatcttc aggtcggcgt agtgccgctt
     3121 gatgacgatg acgaggtagg tcttgagcgc gcacttgttc tcgaatttgc ggagaacggc
     3181 gtagtcatcc tcgatgagct tgatcatgat gaccgagcgg aagtcttccg catcggcgcc
     3241 gatgaggccg tacttcctgc agacgaacgc ggtagcgctg tcgatcagcg gaagattgtc
     3301 gaggaagaat cgctcccagt ccattcagca cgccgcccgc aacaggaaac cgtacttgtc
     3361 tttggcggtg cgcgattcta gacgaattcc accccgggac gcagtgacaa gccaacggac
     3421 ctacggcagt accgcaaatc cctcggcccg agccgccgcg gcgaggtcga ggtcgagggt
     3481 gacgaaaggg agggaagcgg ggtcctcctg gcacgcgatg agcgcggcag cgagttgagc
     3541 gtccatcgca cgaatctcca ccgtcacccc attcttcagg cctcgatgac ccgtccaacc
     3601 gc
//
LOCUS       DATLVW010000250         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_588795, whole genome shotgun sequence.
ACCESSION   DATLVW010000250 DATLVW010000000
VERSION     DATLVW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588795"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..417)
                     /locus_tag="VNN25_07965"
     CDS             complement(121..417)
                     /locus_tag="VNN25_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91500.1"
                     /translation="MSLEDTFSNLASFLNSLDTEGEQKFELSHSFVIICPDCGKNIAF
                     RGRCPKCGGNSWIPAGHTGGIFEQMKAQRWRTEMRSDDDEEPAARDAVEARPDA"
     gene            complement(436..1842)
                     /locus_tag="VNN25_07970"
     CDS             complement(436..1842)
                     /locus_tag="VNN25_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXH91501.1"
                     /translation="MRKPLGPIVVVLAWIYTAAHFVASGIKQPLGNLSGDFLASFPSW
                     RLSVLLGRQDLFKGSLADEWARMVDPKLAWVYAVARFVFSGIKRGLLASFPSWRLSVW
                     QGRLDLFKNILAELARMVWDPHPLWHYGPVEHLVTLPLFAFSDLRSAYTAWLIANYAF
                     LIGILVLGFRVFHSGRSKWIWRSAVAIAVLNYGPLYEALTQRNIEIFELLLIFAAFAL
                     VLRGRQTASGFAIGLAAMTKFLPLIFLPYFVVKRRFRTLAASMLVIALIAVATEAVFG
                     WRYSGIVLQLRRGGLIKSELDQSLAGMVIRLFVWTRSPLSPAMLISRGAIVLALAGLC
                     WLFLRARNCIAIEDIEWSTLIVAMVLLPPHNENYYFVLLLFPYLALLARDLRPGAPAH
                     RARRWWLVISFIFTGTVVPMSALSALTGKNIFSIYLQSGIPFIGAAVLATICVRALLD
                     ECIVSRDRQDNPGTPHAV"
     gene            complement(1839..>2073)
                     /locus_tag="VNN25_07975"
     CDS             complement(1839..>2073)
                     /locus_tag="VNN25_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91502.1"
                     /translation="RIKSNTTIAFEIWRELMKLRDLHEGQDVIVRLNPVVYGSVHNTN
                     DPIFEEIERNLGIHLVFKPDDSLHHEQFDVMRI"
BASE COUNT          464 a          620 c          629 g          360 t
ORIGIN      
        1 cgttgtcggt tgtcggttag ttgcgcttcg cgcgttgcgt ccgatgtcta gccggttttt
       61 cttttgacaa cgcgcgaagc gcaatcaacc gacaaccgac aacgcgccgc aggcgcagat
      121 tcaagcgtct ggccgtgctt caactgcatc acgcgctgcc ggctcctcgt catcgtccga
      181 gcgcatttcc gtccgccagc gctgcgcttt catctgctcg aagatcccgc ccgtgtgtcc
      241 ggccggaatc cacgagttgc cgccgcactt cgggcagcgt cctcggaacg cgatgttctt
      301 cccgcaatcg ggacagatga tcacgaacga gtgcgagagc tcaaacttct gttccccctc
      361 agtgtcgagg ctgttcagga acgaagcaag gttgctgaag gtatcttcga gtgacattgc
      421 ttgctgctca gccgctcaaa ctgcatgcgg cgtgccgggg ttgtcttgcc tgtcacgaga
      481 aacaatgcat tcgtccagga gcgcgcggac gcagatcgtc gccagaactg cggcgccgat
      541 gaaaggaatg cctgactgga ggtatatcga aaagatgttc ttgccagtca gcgcgctgag
      601 ggcggacatc ggcaccacag tccccgtgaa gatgaacgag atcaccagcc accaccgtcg
      661 ggcacgatgc gcgggagcac cgggccggag atcgcgcgcc aggagcgcga ggtaggggaa
      721 cagcaggagc acgaagtagt aattctcatt gtgcggcggc agcaggacca tcgccacgat
      781 cagcgtgctc cactcgatgt cttcgatcgc gatgcagttg cgcgcccgca gaaacaacca
      841 gcagaggccc gcgagggcaa gaacgattgc gccgcggctg atgagcatcg cgggcgaaag
      901 gggggaccgg gtccagacga aaaggcggat gaccattccg gcgagactct gatcgagctc
      961 gcttttgatc aaacctcctc gcctcagttg aaggacgatt ccgctgtagc gccatccgaa
     1021 gactgcctcc gtcgcgacgg cgatcagcgc gatcacgagc atcgaagcgg caagcgtccg
     1081 gaacctgcgc ttcactacaa aatacggcag gaagatcagc ggcaggaact tggtcatggc
     1141 cgcgaggcca atcgcgaatc cggaggcagt ctggcgaccg cgcaacacca gcgcgaaggc
     1201 cgcgaagatc aggagcaact cgaaaatctc gatgttgcgc tgggtgagag cctcgtaaag
     1261 aggcccgtag ttgagaaccg caatcgcgac agccgaccgc catatccatt tcgagcggcc
     1321 gctatggaac acgcgaaacc cgagtaccag aataccgata agaaaggcat agttggcgat
     1381 cagccaggcc gtgtacgcgg atcgaagatc gctgaacgcg aacagcggaa gcgtgacgag
     1441 atgttcgacc ggtccgtaat gccagagagg atgtgggtcc cagaccatcc gcgccaactc
     1501 cgccagaatg ttcttgaaca ggtccaggcg gccctgccaa acgctcaggc gccacgacgg
     1561 aaaactggcc agaaggccgc gtttgatgcc cgagaagacg aagcgcgcaa cggcgtagac
     1621 ccatgccaat tttgggtcga ccatccgtgc ccactcgtcg gccagactac ccttgaacag
     1681 gtcctgccga ccgagcagaa cgctcaggcg ccacgacgga aaactggcca ggaaatcgcc
     1741 agagaggttg ccgagcggtt gcttgatgcc cgacgcgacg aaatgggccg cggtatagat
     1801 ccacgccaaa accaccacga tcgggccgag gggtttcctc atatccgcat cacatcgaac
     1861 tgctcgtgat gcagtgagtc atccggcttg aagaccaggt ggatcccgag gttccgctcg
     1921 atctcttcaa aaatcggatc gttggtgttg tgaaccgagc cgtagacgac cgggttgagc
     1981 cgcacgatca cgtcctgacc ttcgtgcagg tcgcgcagtt tcatcagctc gcgccagatc
     2041 tcgaaggcga tcgtcgtgtt cgacttgatc cgg
//
LOCUS       DATLVW010000251         4188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_54310, whole genome shotgun sequence.
ACCESSION   DATLVW010000251 DATLVW010000000
VERSION     DATLVW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54310"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1584)
                     /locus_tag="VNN25_07980"
     CDS             complement(115..1584)
                     /locus_tag="VNN25_07980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH91503.1"
                     /translation="MASGDSLGISGLGPGKVLDGKYEILTRLGGGGMGDVFKAQHVHL
                     NVFRCIKVMKEAMLADEGYRARFLREARLATQIHHPNIAVVHDFFIGDGGSYMVTEFI
                     DGTTVRQWGRSNGPFPLPLAADVAVQVLAGLDHIHRRGLLHRDISSDNVMLSYDEDGS
                     FIAKIIDLGIAKDVNTPSGEMATQAGMLIGNPKYMSPEQLGSIEDGEQLDGRADLYAL
                     GVVLYEMLLGVPPFASETPQGYIMKHLTQQPRRFRDAKPDGDWSASLEAVVLRALEKD
                     RRRRFADAREMSAALQPFLSAPAGTLTRDAIARLRRGPMKTFNSAVEWRKTKAIDTIA
                     AYRDFLGKVPEGSDTTEAKARLFELELIAGVRAREAEGDRDALQRLAEAHPPGSVVGD
                     AAREALGRVSRSPERQIEEEREFQQAWETGTAAAWTKFIDEHPSSPRTEEAKRCRQEA
                     VDFELASSLDTAAMWRAFLKTWPEGRHRLDADIRLRAKK"
     gene            complement(1798..2223)
                     /locus_tag="VNN25_07985"
     CDS             complement(1798..2223)
                     /locus_tag="VNN25_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91504.1"
                     /translation="MAVQIHQREARTTLDIDVAVSSRESIPRDALSAAGFRIHKSFEH
                     SENWYAADGTPVQFTDDPMLARAVANAEEIRIEDVTLRVIRIVDLLHEKVRAGSDPAR
                     RRSKRLQDLADAEALIEASPELAGELSPDERALLGSLLR"
     gene            complement(2395..2973)
                     /locus_tag="VNN25_07990"
     CDS             complement(2395..2973)
                     /locus_tag="VNN25_07990"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HXH91505.1"
                     /translation="MSTKLRTITRTLLVCILLAGMAVGGEGLWIYAKARLAQLLLEIS
                     WRSALAGEQLKPWPWADTHAIARLTIEHDGAAVIVLDGASGRTMAFGPGHLDGTAMPG
                     DIGNCVITAHRDTHFAPLRYLGPGDIVTLQRPDGRVVRYRVQATRVVRKNDTSVLRQD
                     ARTRLTLITCYPFDAVRPGTPLRWVVIAQKVA"
     gene            complement(3116..>4188)
                     /locus_tag="VNN25_07995"
     CDS             complement(3116..>4188)
                     /locus_tag="VNN25_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH91506.1"
                     /translation="RLPRETIFIIDGSGSMEGASMEQAKAALLLALDDLTPSDTFNII
                     DFDSEARPLFKESSAADGPSIAAAKQFVGTITADGGTEMMSALRIALPEKTNTPSGTV
                     RQVIFMTDGQVSNEQELFSFIHSRLGESRLFTVGIGAAPNSHFMRNAARFGRGTFTYI
                     GDVQQVQERMSELFAKLDAPVMTSIEIHSSDATTEAWPERVPDLYRGEPLVVTLRVRD
                     PGTKFTLRGHIGNDVWTKELTLPAPGEPEGIARLWGREKIESLMDHLTDGSDPKGIEA
                     AVIAVALQNHLVSQYTSLVAIDQTPQGLNPSCKPELIPVDNTVTDPQQGTLPQTATPA
                     ALMLMIGLALVAVAGMALRLMR"
BASE COUNT          732 a         1364 c         1295 g          797 t
ORIGIN      
        1 acaaaggcac aaaggtcaca gagacccctc cgtgtccttt gtgtctctgt ggtcgccttt
       61 gcttcggcga ccacgataaa ccgtccaaat tgacccacaa cccgggccgg atcatcactt
      121 cttcgcgcgc aaccggatat ccgcatccag ccgatgacgt ccctcgggcc acgttttcag
      181 aaaggcgcgc cacattgcgg cggtgtcaag ggacgaggcc agctcgaagt cgactgcttc
      241 ctggcggcaa cgcttggctt cctccgttcg cggcgagctc ggatgctcat cgatgaactt
      301 cgtccacgcc gccgcggttc ctgtctccca cgcctgctga aactcgcgct cctcttcgat
      361 ctggcgctcc ggcgaacggc tcacgcgtcc gagcgcttcc cgcgccgcat cgcccacgac
      421 cgagccgggc ggatgcgcct cggccagccg ttgcagcgca tcccgatcgc cttccgcttc
      481 ccttgcccgc actcccgcga tcaactccag ctcgaagagg cgcgctttcg cttccgttgt
      541 atccgatccc tcggggacct tgccaagaaa gtcacgatac gcggcgatgg tgtcgatcgc
      601 cttcgtcttc ctccattcca ccgccgagtt gaaggtcttc atcggcccgc gccgcagacg
      661 cgcaatcgcg tcgcgcgtga gcgtgccggc cggcgcggag aggaacggct gcagcgctgc
      721 cgacatctcg cgcgcgtcgg cgaagcggcg gcgacgatcc ttctccagtg cccgcagcac
      781 aacggcttcc agcgacgccg accaatcacc gtccggcttg gcgtcgcgga aacggcgcgg
      841 ctgctgcgtg aggtgtttca tgatgtagcc ctgcggcgtc tcgctggcga acgggggcac
      901 gccgaggagc atctcgtaca acacgacacc gagcgcgtag agatcggcgc ggccatcgag
      961 ctgttcgcca tcctcgatgc tgccgagttg ctccggcgac atgtacttcg gattgccgat
     1021 gagcatcccc gcctgcgttg ccatctcgcc cgacggtgtg ttgacgtctt ttgcgatgcc
     1081 gaggtcgatg atcttggcga tgaaggagcc gtcttcatcg tacgagagca tcacgttgtc
     1141 ggacgaaata tcgcgatgca gcaggccgcg gcggtgaatg tgatcgagtc ccgccagaac
     1201 ctgcaccgcg acgtccgccg ccagcggcaa cgggaacggc ccgttcgatc tcccccactg
     1261 ccggaccgtc gtgccgtcga tgaactccgt caccatgtag ctgccgccat cgccgatgaa
     1321 gaagtcgtgg acgacggcga tgttgggatg gtgaatctgt gtggccagac gggcctcgcg
     1381 caggaatcgc gcgcgatagc cttcgtccgc aagcatcgct tccttcatca ccttgatgca
     1441 gcgaaagacg ttgaggtgaa cgtgttgcgc tttgaagacg tcacccatcc cgccgccgcc
     1501 gaggcgtgtc agaatctcgt acttgccatc gagcaccttg ccgggcccga gcccggaaat
     1561 gccgagcgag tcaccgctgg ccatggcttc tcttgcccct cggtgatcag aataccgtga
     1621 aaggaaggcg ggcgggactg cgaggcgcgc cgggtggtgg gtcaatttga aaaaccggta
     1681 accacagagg cacagaggtc acagagatcc cctctgtgtt ctctgtgtct ctgtggttgc
     1741 ctttgcgtcg gcgaccacga taagccgtcc aaattgaccc attacgggcc gccgaaatta
     1801 ccgcagcaag cttccaagca atgcccgttc gtccggactc agctctcctg ccagctcggg
     1861 gctggcttcg atgagcgcct cggcatcagc cagatcctga agacgcttcg atctccgtcg
     1921 tgccggatcg ctcccggcac gaaccttctc gtggagcaga tcgacgatgc gaatgacccg
     1981 aagcgtaacg tcctcgattc ggatctcctc cgcgttcgca acggctcgcg ccagcatcgg
     2041 atcatccgta aattgaacgg gcgtgccatc agcggcatac cagttctctg aatgctcgaa
     2101 gctcttatga attcgaaagc ccgcggcgct cagtgcgtcc ctcggaatcg attcgcgcga
     2161 cgagactgcg acgtcgatgt cgagcgtcgt ccttgcttcc cgctggtgga tctgaacggc
     2221 caagcctccg atgatcgcgt actcagtgtg tgaatcgcgg agaacccggc acaacgtaag
     2281 gagtgaaggc cgcttactct cttccatttc acctcgcaac cagtgccact ggcgatccag
     2341 aagaaaaccg tgggcgagac cagagctcat gcgcacccat tctacgcgga cccctcacgc
     2401 caccttctgc gcgatgacca cccaccgcag aggagttccc ggccttaccg cgtcgaacgg
     2461 gtagcaggtg atgagcgtga ggcgcgtgcg ggcatcctgg cggaggactg acgtgtcgtt
     2521 tttgcggacg acgcgcgtgg cctggacgcg gtagcggacg acgcggccgt cggggcgctg
     2581 gagcgtgacg atgtcgccgg gaccgaggta gcggaggggg gcgaagtgcg tgtcgcgatg
     2641 cgcggtgatg acgcagttgc caatgtcgcc tggcatcgcg gtgccgtcga ggtgtcccgg
     2701 tccgaatgcc atcgtccgcc cgctcgcgcc gtcgaggacg atcaccgcgg cgccgtcgtg
     2761 ctcgattgtc aggcgtgcga tggcgtgcgt gtccgcccac ggccacggct tgagttgctc
     2821 tccagccagc gcgctgcgcc acgagatttc caggagcagt tgcgccagcc gcgctttcgc
     2881 gtagatccag agcccctccc cgccgaccgc catgccggcg aggaggatgc agacgagaag
     2941 ggtgcgagtg atggtgcgga gtttcgtgga catgggttcc tcggggggtg gggggttggg
     3001 tgtggggtgt gggagattag aaagacgagc gcggggcggc ggagagcgga aagcggagtc
     3061 gaatgactaa atcagacatg ttcaactcca ccccccacac cccaccccct actccctatc
     3121 tcatcaacct caaagccata cccgccaccg ccaccaacgc cagtccgatc atcagcatca
     3181 gcgctgcggg ggttgccgtt tgcggcagcg tgccttgctg cggatccgtc accgtgttgt
     3241 cgaccggaat cagttcgggc ttgcacgatg gattcagtcc ttgcggcgtc tggtcgatgg
     3301 cgacgaggct tgtgtattgc gacacgagat ggttttgcag cgccactgcg atcaccgccg
     3361 cttcgatacc tttcggatcg ctgccgtcgg tgagatggtc catcagcgac tcgatctttt
     3421 cgcggcccca cagtctcgcg attccttccg gctcgcccgg cgcaggcagc gtcagctcct
     3481 tcgtccaaac gtcattgccg atgtggccgc gaagcgtgaa cttggtaccg ggatcacgca
     3541 cgcgcaacgt aaccacgagc ggctcgccgc gatacagatc cggcacgcgt tccggccacg
     3601 cttccgtcgt cgcatcgctg ctgtgaatct cgatcgacgt catcaccggc gcgtcgagct
     3661 tcgcgaacag ctccgacatc ctctcctgca cctgctgcac gtcgccgatg taggtgaacg
     3721 tgccgcggcc gaagcgcgcc gcgttgcgca tgaagtgcga gttcggcgcg gcaccgatac
     3781 cgacggtgaa gagacggctc tcgccaaggc ggctgtgaat gaaggagaag agctcctgct
     3841 cgttgctgac ctgtccgtcg gtcatgaaga tcacctggcg cacggtgccc gacggcgtat
     3901 tcgtcttctc cggaagcgcg atgcgcaacg cgctcatcat ctcggtcccg ccgtccgcgg
     3961 taatcgttcc gacgaactgt ttggccgccg cgatcgaagg tccgtcggcc gcgctgctct
     4021 ccttgaacag cggacgagcc tcggaatcga agtcgatgat gttgaacgtg tcgctcggtg
     4081 ttagatcgtc gagggcgagc agcagcgccg ctttggcctg ttccatcgac gcgccttcca
     4141 tcgagccgga gccatcgatg atgaagatcg tctcgcgcgg cagacgga
//
LOCUS       DATLVW010000252         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_588895, whole genome shotgun sequence.
ACCESSION   DATLVW010000252 DATLVW010000000
VERSION     DATLVW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588895"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1094..1483
                     /locus_tag="VNN25_08000"
     CDS             1094..1483
                     /locus_tag="VNN25_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HXH91507.1"
                     /translation="MNREIAIGLGGEALVVSYERARLSSLGCDTLAQRVQHVAATIGD
                     GLGYDVLSFTADGRDRFIEVKTTQLGPMTPFYITDSEISFSTENHDRYSLYRVYDFAA
                     RPSMFSLDGRVESSCTLRATQFMAMPR"
     gene            1544..2530
                     /locus_tag="VNN25_08005"
     CDS             1544..2530
                     /locus_tag="VNN25_08005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXH91508.1"
                     /translation="MPTPFSVLDLAFVTAGSTPSDALRNTIDLARHVERWGFRRFWLA
                     EHHNMIGIASAATAVVIGYVAAGTRTIRVGAGGIMLPNHAPLVIAEQFGTLASLYPDR
                     IDLGLGRAPGTDQRTLRALRVSPVSSDTFPQDVIELQSYFAPLREGQLIQAVPGTGLN
                     VPLWILGSSLFGAQLAAELGLPYAFASHFMPGALMEALAIYRARFKPSSQLDRPYAMA
                     GVNVFAAETDAEGRRLFTSAQQSFANIFRGTRGRLPPPIDDIETYWTPQEKLQASAML
                     ACSFAGSRDTVGRGLAGFIERTGVDEVIVASAIYDHAARLRSYEIVADIANA"
BASE COUNT          515 a          872 c          904 g          553 t
ORIGIN      
        1 tactgaccct taagggcgtc agggcgggtt cacaaagtga acagtgaggc agaggatgac
       61 ccacccttgg gggctgcatc ggagtgaagg ctttctatcc gagggacaat gtccgtcccg
      121 agttccggcc ttgacgccga ggtggctcga ctcatcagac cgagggtttt atggactacc
      181 cgtggtggac gatcgacgcc gcagattacg gcttccgaga agaagatgag atccgctgtc
      241 tcgactgcgc cgaacgagac agacaggccc tcggccgcga gctcaccgac tccgagatag
      301 ccgatgcagg aggcgtcagt tgcctctcgt gcggtcgaag ttggcggcca gcctaggagt
      361 ttgcgcggca gaaactgcaa agccgccctt cacgcgggca agagcgtcgc cgaattttga
      421 cctccgccct ggcagcactc gggcgaggac gcccgagtgc ccgcggtcga gacgaccgca
      481 ctccttgcga cgtgcgcggt gaggagtgtg ggcgtctcgc ccgcggccat cggggcgtcc
      541 cgcctcggtg gttggatctg gcccgatagt tccaacggct tgaaccagaa acccgatcga
      601 gtctcgcgat ggagcggctg ctatcctggc ttcgtggcat ctggtcagga ctggaacgag
      661 ctcgaggtag aggcgatcgt cgcggactac ttctcgatgc ttgagcaaga gttgcgcggc
      721 gttccttaca acaagagcgc gcatcgccgg gcgctgaagc ctctgctccg cggccggtcc
      781 gatggatcga tcgagataaa gcatcagaac atcagtgcca ttctgatcga agccgggcat
      841 ccttacattc gcggatacaa gccggcatcg caatatcaag gcttactcag cgatgtagtc
      901 ctcgatcgcc tggacggcga cgcggcgctg cggcatctcg ccgtcgaatt cgccgaggca
      961 gtggttccgc ctccaaggat cggcaacatg ctctccttga tgacaaaacc gcccgaccta
     1021 ctagagtcag cacgtcttgc ccttgcttca cagaaacgga gaaggcaggc acgaacgatc
     1081 gattacctcg cgcttgaatc gcgaaatcgc catcgggctt ggaggagagg cactggttgt
     1141 cagctacgag cgcgcgcgac tcagctccct cggatgcgac acgctcgcgc aacgagtgca
     1201 gcatgttgca gccacgattg gcgacggatt gggctatgac gtcctctcct tcactgctga
     1261 tggccgcgat cgattcatcg aagtgaagac cacgcagctc ggaccaatga ctccgtttta
     1321 catcaccgat agcgagattt ctttctcaac cgagaaccac gaccgttact cgctctaccg
     1381 cgtctacgac ttcgcagcac ggccatcgat gttttcgctc gatggacgag tcgagagcag
     1441 ttgcacgctt cgcgcgacgc agttcatggc aatgccgcgg tgaatctgga ctgagcgcaa
     1501 gtgaacaatc cccccggtga tcttcttgca ctgctcctga ccgatgccga cgcccttctc
     1561 cgttctggat ctcgcgtttg tcactgccgg ctccacgccc tcggatgcgt tgcggaacac
     1621 gatcgatctg gcgcggcatg tggagcggtg gggcttccgg cgtttctggc tcgcggagca
     1681 tcacaatatg atcgggatcg ccagcgcggc gacggcggtg gtcatcggct atgtcgcggc
     1741 ggggacgcgc acgatccgcg tcggggcggg cgggatcatg ttgccgaatc atgcgccgtt
     1801 ggtgatcgcc gagcagttcg gaacgctcgc ctcgctttac ccggaccgca tcgacctcgg
     1861 gctcgggcgc gctccgggaa ccgatcagcg cacgttgcgt gcgttgcgcg tctcaccggt
     1921 cagctccgac accttcccgc aggacgtgat cgagcttcag tcgtactttg caccacttcg
     1981 cgagggacag ctcattcaag ccgtgccggg caccggtctc aacgtcccgc tgtggattct
     2041 gggctcgagc cttttcggtg cacagctcgc ggcggagctc gggctcccct acgcgttcgc
     2101 ttcgcacttc atgccgggag cgttgatgga ggcgctggcg atctatcgcg cgagattcaa
     2161 gccgtcgagc cagctcgatc gtccctatgc gatggccggc gtgaacgtct ttgccgccga
     2221 gaccgatgcc gaggggcgcc gccttttcac ctccgcgcaa cagtcgttcg cgaacatctt
     2281 ccgcggaaca cgtggacgtc tgccgccgcc gatcgacgac atcgagacgt actggacgcc
     2341 ccaggagaag ctgcaggcgt ctgcgatgct ggcctgctca ttcgccggtt cccgcgatac
     2401 ggttggccga ggactggccg ggttcatcga gcggaccggt gtcgatgagg tcatcgtggc
     2461 gtcggcgatc tacgatcacg cggcgcggct tcgctcgtac gagatcgtgg cggacatcgc
     2521 aaacgcgtag taaccgggcg gaagcatgaa ccgcagaggc aatctgggga tacatcgaac
     2581 tgtaacgctg ccagcaacat gcgcgcgcag cgtgacacag ggggtgccca cgtgcgcacc
     2641 gaggttccag catttgctgc agtgggacac ggtcggtgtc ccagtggtac atggccggtg
     2701 tcccagtggg acacggtcgg tgtcccagtg gtacatggtc ggtgtcccag tggtacacgg
     2761 tcggtgtccc agtggtacac ggtcggtgtc ctagtggtac acggtcgctg tcccagtggt
     2821 acacggtcgg tgtcccagtg gtac
//
LOCUS       DATLVW010000253         5695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_10014, whole genome shotgun sequence.
ACCESSION   DATLVW010000253 DATLVW010000000
VERSION     DATLVW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10014"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="VNN25_08010"
     CDS             <1..2533
                     /locus_tag="VNN25_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91509.1"
                     /translation="YELWLAPAGGVPTLIRVTSDTSFTATVPLGRLEWYVRAIFGGCP
                     ATESAHQTFTYTAPPECTSQRPLLIAPAEGERLTSPVSFEWRAVPGATSYELYVDGVL
                     AATTTSPRAPGISVPPDEQRWRVRAHLAEACGALDSAESRFAVIAPSSSCTPLDPPLL
                     TAPGQISSGVTARIQWTFVAGATAYVVQISSDPLFPLASTSSSIITTRELPFTFTNES
                     NVPAARYVRVSAIDTECVLPSSGPFSPSAVVIVLPRTGAAGAALLSDPTDVQYTLNIT
                     ADFAGLSFTATPSAPWITVTPASGIVPPGGQTLLAVAHTAGLPAGASTGTVVITTAAP
                     AGSRAALGNPPPISVPITVSNISGVTTKPKSTPPPDSLTIPAVANVKNFIVRYQSDVC
                     VTNTSAQAIKYEINFTPTGLPGLSQGQTTNVSIEPGATLALNDIVTTWFGGLTSSGTL
                     EIRPLTEIDTSTSSAPASGLANRITFASSRTFAVTADGATYGQYIPAVPYANFVSQGA
                     VLSLQQIAQSDKYRTNLGLVEGSGEPVSVQVRIFDSTGTKRGDFNVDLNGGQHTQLNE
                     VLKEHGIALDDGRIEVEVTKGTGRVTAYASVIDNDTNDPLLVPPVTIAGAGDRTWVLP
                     GVADLTGGSSNWRTDVRVFNAGKDPVDLTLAFYSRNGGPAATRTISLAAGGVQQLDRV
                     LTSFFGISQDAGALHVSSAVPSRLVVTARTYNDTGQGAYGQFIPAVTPEEAVAAGSRP
                     LQILQVEESGQYRSNVGFAEVSGNAVTLEVSVFQPGHNDPALLEVKLGPNEFRQIDSL
                     LSTLGLAETFNARISVRAVSGEGRATAYLSLIDAKSGDPTYVPGQ"
     gene            complement(2711..3283)
                     /locus_tag="VNN25_08015"
     CDS             complement(2711..3283)
                     /locus_tag="VNN25_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91510.1"
                     /translation="MNANQETMNPSAAADLPQAAPTLSPEGVVDQLRATCAQIGEITP
                     LTVAQRRALRGRIRTSNPIEQASINVLGALDNVSQAVGQQADAVRQLQDETNRWTAVE
                     DELRTMLNGISGANLIRRYRISLIAGQAYNIGSQLARDPANAVLVPHVQEVKRLRSFQ
                     RRHKKASSAPGSQQSPEPGVPPVASDSPQM"
     gene            complement(3652..4116)
                     /locus_tag="VNN25_08020"
     CDS             complement(3652..4116)
                     /locus_tag="VNN25_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91511.1"
                     /translation="MQRSLLRDHSDAGAAEIGSERDRCSIRPVLSNVQPDVRGAQPDM
                     CGAQPEVRGVQPDVRGVQPDVCGVQPDVCGVQPDVCGVQPDVCGAQPDVCGVQPDVRG
                     VQPDVCGVQPDVCGVQPNLCGVQPARTVIGRAREVIESTGTVESSRIVMTSV"
     gene            4655..>5695
                     /locus_tag="VNN25_08025"
     CDS             4655..>5695
                     /locus_tag="VNN25_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXH91512.1"
                     /translation="MLLAVSVVPGARASCLIPYWSPTYSTSRTADFVIAADLDGDGKP
                     DIAGNTLTEIFVLRNDGTGHFTVPVVVYTGTIQGSIVAEDFDGDGKMDLAFAGASSLF
                     VLPGHGDGTFGSAIESPITIVPVQLSSGRFDAGSSIDLIAYDPAARVLVVFSNDGTGH
                     FVELSRTPIAANARAMVTGDFDGDGNLDAVVGYTASYGYDAFYGHGDGTFESPQTIWG
                     LYQPNLLRAEKIDGTGLTDLIVGRYYDLAIIRNLGGRNFGDPLRYDSYGTTEALTTAD
                     VTGDAVPDIVGSTSCDFFSDTSSGIGTITYVRDNIYDCYYETNTGALAAADFDGDGRL
                     DLVLSLADTSDPS"
BASE COUNT         1033 a         1942 c         1733 g          987 t
ORIGIN      
        1 gtacgagctg tggcttgcac cggcaggcgg cgtgccgacg ctgattcgtg tcacctcgga
       61 cacgtcgttc accgcgaccg taccgcttgg gcgcctggag tggtacgtgc gcgcaatctt
      121 cggaggctgc cccgcgaccg aatccgccca tcaaaccttc acgtacacgg cgccgcccga
      181 atgcacgagt cagcggccgc tgctcatcgc gcctgccgag ggagaacggc tcacgtcgcc
      241 ggtttcgttc gaatggcgcg cggtgcccgg ggcgacgtcg tacgagctct acgtcgacgg
      301 cgtgctggct gcgacgacaa cgtctccacg tgcccccggc atctccgtac cgccggacga
      361 gcagcgctgg cgggtgcgcg cccacctcgc cgaagcttgc ggtgcgctcg actccgcgga
      421 gagccgcttt gccgtcatcg cgccgtcctc gagttgtacg cccctcgatc cgccgcttct
      481 caccgcgccg gggcagatct cctccggcgt caccgcacgg attcagtgga cgttcgtcgc
      541 cggcgcgacc gcctatgtcg tccagatctc cagcgacccg ctattcccgc tggcgtccac
      601 gagctcttcg atcatcacca cgcgggaact gccgttcacg ttcaccaacg aaagcaacgt
      661 gccggccgcg cgctacgtgc gcgtgtccgc gatcgatact gaatgcgtgc tgccgagtag
      721 cggtccgttc tcgccgtctg ccgtggtgat cgtcctgccg cggaccggag ccgccggtgc
      781 ggcgttgctg agcgatccta cggatgtcca gtacaccctg aatatcaccg cggatttcgc
      841 ggggttgagc ttcaccgcca cgccctcggc accctggata accgtcacgc ccgcgagcgg
      901 catcgtgccg cctggaggcc agacgctgct cgcggtcgcg cacactgccg gtctccctgc
      961 cggcgcgagc accggcactg tcgtcatcac cacggccgcg ccggccggct cgcgggccgc
     1021 gctcgggaac cctccaccca tcagtgtgcc gatcacggtc agcaacattt ccggcgtcac
     1081 cacgaaaccg aagagcacgc cgccacccga ctcgctgacc atccccgccg tggccaatgt
     1141 gaagaatttc atcgttcgtt atcagtcgga cgtctgtgtc accaacacct cggcacaagc
     1201 gatcaagtac gagatcaact tcactcccac cggcctgccg ggcctcagcc aggggcagac
     1261 cacgaacgtc tcgatcgagc cgggcgccac gcttgcgctc aacgacatcg tgacgacgtg
     1321 gtttggcggc ctgacctcga gcggtacgct ggagatccgt cctctgacgg agatcgacac
     1381 gtcgacgtcg agcgctcctg ccagcgggtt ggcgaacaga attacgttcg cttcctcccg
     1441 caccttcgcc gtgacggccg acggagctac gtacggacag tacattccgg ctgtccccta
     1501 tgcgaatttc gtctcccagg gagccgtcct ctccctgcag cagatcgcgc agtcggataa
     1561 gtaccgcaca aacctcggct tggtcgaagg ctccggcgaa ccggtctcgg tccaggtacg
     1621 catcttcgac tcgacaggta cgaaacgggg cgacttcaac gtcgatctga acggcgggca
     1681 gcacacccaa ctcaatgaag tcctcaagga gcacggcatc gcccttgacg acggccgcat
     1741 cgaagtggag gtcaccaagg gcaccggcag agtgaccgcc tacgcctcgg tcatcgacaa
     1801 cgacaccaac gacccgctgc tcgtgccccc ggtgaccatc gccggcgccg gcgacaggac
     1861 gtgggtcctg cccggagtgg ccgacctcac cggcggatcg agcaactggc ggacggacgt
     1921 gcgcgttttc aatgccggaa aagaccccgt cgatctcaca ctcgcgttct actcgaggaa
     1981 cggcggcccg gcggcaaccc ggacgatttc gctcgccgct ggcggagtgc agcagctcga
     2041 tcgcgtgctc acgtcattct tcgggatctc gcaggacgcc ggcgcgctgc atgtttcgag
     2101 cgccgttccg tcccgtctgg tcgtcaccgc acgcacatac aacgacacag gacagggagc
     2161 ctatgggcaa ttcatccccg ccgtcacgcc ggaagaggcg gtggcggccg gctcacgccc
     2221 actgcagatc ctgcaagtgg aggagtccgg gcaatatcga tccaacgtcg gcttcgccga
     2281 ggtgagcgga aacgcggtaa cgctggaggt gtcggtcttc cagcccggtc acaacgaccc
     2341 ggcgttgctc gaagtgaagc tcggtccgaa cgagttccga cagatcgact cgctgctctc
     2401 cacacttggc ctcgccgaga ccttcaacgc acgcatcagc gtgcgcgccg tatccggcga
     2461 aggacgagcc acagcgtacc tgtcgctgat cgacgcgaag agcggcgacc caacgtatgt
     2521 ccccgggcaa taagagtcag gagctcatca cgagactccg agacgccgag cgcggcgatc
     2581 atgaactcgc gtcgggttca accaccgggg cgggacgccc cgatggccgc gggcgagacg
     2641 cccgcactcc tcggcggggg gaaacgaccg ggcgcgcctt tgcgcgcccg gtctcactca
     2701 ctccgatcac ttacatctgc ggactgtcgg acgccacagg cggcaccccg ggttccggag
     2761 actgctgcga gccgggagcc gacgacgcct ttttgtgacg gcgctggaag ctcctcaagc
     2821 gcttgacctc ctgcacatgc ggcacgagaa cggcgttggc cggatcgcgc gccaactgcg
     2881 agccgatgtt gtacgcctgc ccggcgatga gggaaatgcg atagcgccga atgagattcg
     2941 ccccggagat gccgttcagc atcgtccgca actcgtcctc caccgccgtc cagcggttgg
     3001 tctcgtcctg caactggcgc acggcgtcag cctgctggcc gaccgcctgc gagacgttgt
     3061 cgagcgcacc gaggacgttg atcgacgcct gctcgatcgg attggacgta cggatgcgcc
     3121 cgcgcagtgc tcttcgctgc gcaacggtca acggcgttat ttcgccgatc tgcgcgcacg
     3181 tggcgcgcaa ctggtcgacg accccctcgg gggacaatgt gggagccgcc tgtggaaggt
     3241 ccgcggcggc agacggattc atggtttcct gatttgcatt catttcgttc tcctcagaac
     3301 aaatagttgt taaggccgga gggaacgacg agcacttgtg cacgagtgca ggcggccacc
     3361 ggctggaaaa gacggcaacg cgccgctctt ccggttgatg ttttcccgaa gtgcgcgcac
     3421 ttcgagagcc cgcggcagga ggagcaccac tcaactcccg cagggatgag accagtcgca
     3481 ctttccagcg ccgtcgttct cacccgttgg ccgccaggag cgtgggactc tcacgagcgc
     3541 cggctccagt ctcgagaagc tggcaagcca gccgcacgag gccgcgtatt gcagcagagc
     3601 ctggtggggg atgtcaagtg gggcggcgtc gcggtggatt gagttattgg ctcataccga
     3661 ggtcatgacg atccgcgaag actcaactgt ccccgtactc tcaatgactt cccgtgcgcg
     3721 cccgatgact gtccgcgccg gctgaacgcc gcacagattc ggctggacgc cgcacacgtc
     3781 cggctggacg ccgcacacat ccggctgaac gccgcgcacg tccggctgga cgccgcacac
     3841 atccggctga gcgccgcaca catccggctg gacgccgcac acgtccggct ggacgccgca
     3901 cacgtccggc tggacgccgc acacgtccgg ctgaacgccg cgcacgtccg gctggacgcc
     3961 gcgcacctcc ggctgagcgc cgcacatgtc cggctgagcg ccgcgcacgt ccggctggac
     4021 gttggacagc accggccgga tgctgcatcg atcccgctca ctgccgattt cggcggcgcc
     4081 tgcatcactg tggtcgcgca ggagggaccg ctgcacgagc tctcaccgca acccctccaa
     4141 tcgagcgcga tgggagagca caccaacaaa acgaagattg caaggagggc atgaagggtg
     4201 tgtactacgc gtgactttcg tccatgggtc gcttgtttcg cgggtagtag agaccaaggc
     4261 aggtcagggt cacaccttcg gttatcgcca atctcctcga acgcgaccat aactcgcgca
     4321 gcttcattga cttccgtcaa ctacgccgga aagacatcgc ccatgcgctc aggctcgatt
     4381 ccgcgcctaa gttcttgcaa acacatcgct tgctcactta ccggattctc gcagaccaca
     4441 gtgccccgaa tcgtgtccaa gccgtaagtc actcccagac cgaaacttac gcgaaaataa
     4501 tgcggaacga ccgaagtgcg aaggtgtggc cccactcgct ccacaaagca gacggtactc
     4561 ttggtagaat gacgcccagt tggtccttct acaggaatcc ctttgtaacg cccgggacac
     4621 cagggtcgcg aggctgagcc gccttcttgc gctcgtgctc ctcgcggtca gcgtggtgcc
     4681 gggggcccgg gccagttgcc tgatccctta ctggtcgccg acgtattcga catcgcgcac
     4741 ggcagacttc gtcatcgcgg cggatctcga cggcgatggg aagccggaca tcgccggcaa
     4801 cacgctcact gagattttcg tgctccggaa cgacgggacc ggtcacttca cagtgcctgt
     4861 cgtggtttac acaggcacga tccagggaag catcgtggcc gaggatttcg acggagatgg
     4921 caaaatggat cttgcgtttg ccggggcgtc gtccctgttc gtgttaccgg ggcacggcga
     4981 cggcacattc gggtctgcca tcgagagtcc gatcacgatc gttcctgtac agttgtcctc
     5041 gggtcgtttc gacgctgggt cgtcgatcga tctcatcgcc tacgatcccg cggcaagagt
     5101 gctggtcgtc ttctcgaacg acggcacggg acacttcgtc gaattgtccc ggaccccgat
     5161 cgccgccaac gcgcgggcaa tggtcactgg agacttcgac ggcgacggca acctcgacgc
     5221 cgtcgttgga tacaccgcca gctatggtta cgacgcattc tacggacacg gggacggaac
     5281 attcgaaagc ccgcagacga tctggggact ctatcagccg aaccttctgc gcgccgagaa
     5341 gatcgacggc acaggtctga ccgacctgat cgtcgggcgc tattacgacc tggcgatcat
     5401 ccgcaatctc ggcgggcgta acttcggcga tccgttgcgg tacgacagct acggcacgac
     5461 cgaggctctc acaacggctg acgtcacggg cgacgccgtc cccgacatcg tggggtctac
     5521 ttcgtgcgac ttcttctccg acacatcgag cggcattgga acgatcacat acgttcggga
     5581 caacatctac gactgctact acgaaaccaa caccggagcc ctcgccgctg cggactttga
     5641 cggcgacggc aggctcgacc tcgtcctctc gctcgccgat acatccgacc cgagt
//
LOCUS       DATLVW010000254         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_258427, whole genome shotgun sequence.
ACCESSION   DATLVW010000254 DATLVW010000000
VERSION     DATLVW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258427"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1129)
                     /locus_tag="VNN25_08030"
     CDS             complement(170..1129)
                     /locus_tag="VNN25_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HXH91513.1"
                     /translation="MTVIVAFVVVIFALIMISRIARVVPQQNAWVVERLGKYSSTLEP
                     GFHILVPFLDNVRYKHSLKEVAMDVPEQVCITRDNVQVAVDGVLYFKVLDPARASYGI
                     SDYTFAISQLAQTNLRSEIGKIELDRTFEERTNINLMVVSEVDKASEAWGVKVLRYEI
                     KNINPPKDVLAAMEKQMRAEREKRATVLQSEGQRDASINQAEGDKQQVIKASEAKKQQ
                     AINEAEGQSAAIESVAVATANGIKAVAEAIKTAGGFEAMQLRLAEEYIKQFGELAKSG
                     NSMIIPANLSDVGSIIAMATSVFKGAAGDAKVPIRPAVDSTRG"
     gene            complement(1151..1576)
                     /locus_tag="VNN25_08035"
     CDS             complement(1151..1576)
                     /locus_tag="VNN25_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXH91514.1"
                     /translation="MEWWFWVLAAFLLLTIEFFATTAHVGFFAVGAFLVAILVGLGFD
                     IPLWAQLLIFAISSVVLLVFVRPIVVRKLGLSKTHVVDTMVGEQAILLGDLAVAGEGK
                     AEMRGSTWTARNVGEIALLKGQRCVVEKVVGLTIYVRAS"
     gene            complement(1617..2531)
                     /locus_tag="VNN25_08040"
     CDS             complement(1617..2531)
                     /locus_tag="VNN25_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH91515.1"
                     /translation="MRIQAAVVTIFLAATSAFANFREFTDVPKNPALGLAVQHAAEQT
                     LKDFPKLTADNLAISIIDVTKDSTVDRGDWHGDAPFYPASVVKLYIMAEVFHQHLEGD
                     PEVARALGEMIRISDNDATAFLIDVISDTAPGSNLEGRALNKFMEKRRVVNKWFASMG
                     YDISAMAKPWSFGPFGREKQLLGGDARPNRNRSSANSFASLLLWIVRKRAVTPTASDK
                     MLALLERPLNPLRADENQVKEFIGESLPPGSKEWSKAGWTSEVRHDAAYIELPSGRKL
                     ILVIMTRGTADDVKLIPAIAKSVLSEVQ"
     gene            complement(2662..>4172)
                     /locus_tag="VNN25_08045"
     CDS             complement(2662..>4172)
                     /locus_tag="VNN25_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXH91516.1"
                     /translation="GLGNLIYLYDDNDVTLDGDKRLSMSEDVKKRFEAAHWHVQKIDG
                     HDRDAVRKAIRKAQKVTDKPSMIMAKTVIGFGSPKFHGTSRAHGEPLGPEEAAATKAA
                     LGWPAEPAFLIPDEVQAEFTRKVKKLARARKQWDKGMAEWRARNPQLAAEWDGYWSHT
                     LPEGYELQLAEAGKTDKPIATRELSNQVIQKIGDIAPFVVGGAADLASSTKTNFKNGG
                     SIERAKDDTNDPPPFDEFKGKNLHFGIREHSMGAEINGITLYGPLRAHGSTFLIFSDY
                     MRPAIRLAALMKIPSIFVYTHDSVFLGEDGPTHQSVEQLFSLRLIPNMTLFRPADGVE
                     TGMAWAYAIGSKHEGPTLLVFTRQKVDIIPHGADFDPHQIWRGAYVLDGYADGDFTFI
                     ATGSEVTLAVRTAELLRQEGVRARVVSAPSLDLFDRQDAAYQNEILGDRSRIVAIEAG
                     RSSGWYKYVNHDALIIGIDRFGASAPLADIEKHLGFTPEAVAEKVRGWRKPA"
BASE COUNT          738 a         1322 c         1283 g          829 t
ORIGIN      
        1 gagcgtcatt gatgagaatc gctcgcgccg gacgaacacg atcaacgaag tttgagatcc
       61 ttcgatcgtc ctacgccgcc gatgcctgtc ggggggtgag tgcagcgtaa agtcggcggc
      121 tccggcgact caggatgaca cgactgcaac gctgtgcacc taacgaggat tacccccgcg
      181 ttgaatccac cgccggccgg attggcacct ttgcatcgcc tgccgcgcct ttgaacacgc
      241 tcgtggccat ggcgatgatc gagccgacgt cgctcaggtt cgcggggatg atcatcgagt
      301 tgccgctttt ggccagctcg ccgaactgtt tgatgtactc ctcggcgagg cggagctgca
      361 ttgcttcgaa tccgcccgcg gtcttgatcg cttcggctac ggccttgatc ccgttcgcgg
      421 tggcgacagc gactgactcg atggccgcgc tctgcccctc ggcctcgttg atcgcttgct
      481 gcttcttcgc ttccgacgcc ttgatgacct gctgcttgtc gccctcggcc tgattgatcg
      541 acgcgtcgcg ctgcccttcc gattgcagca ccgtggcgcg cttttcccgc tcggcgcgca
      601 tctgcttctc catcgctgcc agcacatcct tcggcggatt gatgttcttg atctcgtagc
      661 ggagcacttt cacgccccac gcttccgacg ctttgtcgac ttcgctcacg accatgaggt
      721 tgatgttcgt gcgctcttcg aacgtgcgat cgagctcgat cttgccgatc tcgctgcgca
      781 ggttcgtttg cgcgagttgc gagatggcga aggtgtagtc ggagatcccg tacgacgcgc
      841 gcgcgggatc gagcactttg aaatagagca cgccgtcgac cgccacctgc acgttgtcgc
      901 gcgtgatgca gacctgttcc ggcacgtcca tcgccacttc cttgagcgaa tgcttgtagc
      961 ggacgttgtc gaggaacggc acgaggatgt gaaagcccgg ctccagcgtc gacgaatact
     1021 tgcccagccg ctccaccacc cacgcgttct gctgcggaac gacacgcgca atacggctga
     1081 tcatgatgag cgcgaagatg acgacgacga acgcgacgat aacggtcatg acggcctccg
     1141 ttgatgttgt ttacgacgcg cgcacgtaga tggtcagacc gacgaccttc tccacgacgc
     1201 agcgttgtcc tttgagaagc gctatttctc cgacgttccg cgcggtccag gtactgccac
     1261 gcatctccgc ctttccctca cccgccactg ccagatcgcc gagaaggatg gcttgctcgc
     1321 cgaccatcgt atcgacgacg tgggtcttgc tgagtcccag cttgcgcacg acgatcggac
     1381 gcacgaagac gagcagcacg acggacgaga tggcgaagat aagtagctgc gcccacagcg
     1441 gaatgtcaaa tcccagaccg acgagaatgg ccacgagaaa cgccccgacc gcgaagaacc
     1501 cgacatgcgc ggtcgtcgcg aagaactcga tcgtgagcag aagaaatgcg gcgagaaccc
     1561 agaaccacca ttccatatgc gtgtgcggat gaggttacca cgctgcccgc ggcctgttac
     1621 tgcacctcgg acagcacgct cttcgcaatc gccgggatga gcttgacgtc gtccgcggtg
     1681 ccgcgcgtca tgatgacgag gatcagcttt cgtccgctgg gaagctcgat ataggcggcg
     1741 tcgtggcgga cttccgaggt ccatcccgct ttcgaccact ccttcgaacc tggcggcaat
     1801 gactcgccga tgaactcctt gacctgattc tcatcggcgc gaagtgggtt cagcggacgc
     1861 tccagcagcg ccagcatctt gtcgctggca gtgggcgtga cggcgcgctt gcgcacgatc
     1921 cagagcagca gcgaagcgaa cgaattggcg ctcgatcggt tgcggttcgg ccgcgcatcg
     1981 ccgccgagga gttgcttctc gcgtccgaac ggtccgaagc tccagggctt ggccatggcg
     2041 ctgatgtcat agcccatcga cgcaaaccat ttgttcacga cgcgccgctt ctccatgaac
     2101 ttgttcagcg cgcgcccttc gagattcgat ccgggagccg tatcgctgat gacgtcgatg
     2161 agaaacgcgg tggcatcgtt gtcggagata cggatcatct cgccgagcgc ccgagccact
     2221 tccggatccc cctcgaggtg ctgatgaaac acctcggcca tgatgtagag cttgacgacc
     2281 gaggccggat agaacggcgc atcaccatgc cagtcgccgc gatcgaccgt cgagtccttc
     2341 gtgacgtcga tgatgctgat ggcgaggttg tccgcggtca gcttcggaaa atccttcagc
     2401 gtctgctccg cggcatgctg caccgcgagc ccaagcgcgg gattcttcgg cacatcggtg
     2461 aactcgcgga agttggcaaa ggcggatgtg gcggcgagga agatcgtgac gacggcagct
     2521 tgaatgcgca tgggcgaatt gtagcggtcg cgtactcaga tgatgagaag caaagtgcac
     2581 cacagagaca caaaggacac agagttaaag aacccctttg tgtcctctgt gtctctgtgg
     2641 tgacaagggt ttaccaggtc tctaggctgg cttcctccac cccctcacct tctccgccac
     2701 cgcctcgggc gtgaagccga ggtgcttctc gatgtctgcc agcggcgccg acgcgccgaa
     2761 gcggtcgatg ccgatgatca gggcgtcgtg gtttacgtac ttgtaccagc cggaggagcg
     2821 gccggcttcg atggccacga tgcggctgcg gtcgccgagg atctcgttct ggtaggcggc
     2881 gtcctggcga tcgaagaggt cgagcgacgg cgcggagacg acccgggcgc gcacgccctc
     2941 ctggcgcaac agctcggcgg tgcggacggc cagcgtcact tccgagccgg tggcgatgaa
     3001 cgtgaagtcg ccgtcggcgt aaccgtcgag cacatacgcg ccgcgccaga tctggtgcgg
     3061 atcgaaatcg gcgccgtgcg ggatgatgtc gaccttctgg cgcgtgaaca cgagcagcgt
     3121 cggcccctcg tgcttgctgc cgatcgcgta cgcccacgcc atccccgtct ccacgccgtc
     3181 ggcgggacgg aagagcgtca tgttcgggat cagacggagc gaaaagagct gctcgaccga
     3241 ttgatgggtg ggaccgtctt cgccgaggaa gacggaatcg tgcgtgtaga cgaagatcga
     3301 cggaatcttc atcagcgcgg ccagccggat cgccgggcgc atgtagtccg agaagatcag
     3361 gaacgtcgag ccgtgcgcgc gcagcggtcc gtacagcgta atcccgttga tctccgcgcc
     3421 catggaatgc tcgcggatgc cgaagtgcag gttcttcccc ttgaactcat cgaacggcgg
     3481 cggatcgttc gtgtcgtctt tcgcgcgctc gatcgatccg ccattcttga agttcgtttt
     3541 cgtcgatgac gcgagatcag ccgcgccgcc gacgacgaac ggggcgatgt cgccgatctt
     3601 ctggatgacc tgattcgaca gctcacgcgt cgcgatcggc ttgtcggtct tccccgcctc
     3661 ggccaactgc aactcgtagc cctcaggcag cgtgtgcgac cagtagccgt cccactccgc
     3721 ggccagttgc ggattgcgcg cgcgccactc ggccatccct ttgtcccact gcttcctcgc
     3781 gcgggccagc ttcttcacct tgcgcgtgaa ctcggcctgc acttcgtcag gaatgaggaa
     3841 cgccggttcg gcgggccagc cgagcgccgc tttcgtcgcc gctgcttcct cggggccgag
     3901 gggctcgcca tgcgcgcgcg aggtgccgtg aaacttcgga ctcccgaacc cgatcaccgt
     3961 cttggccatg atcatcgacg gcttgtcggt caccttctgc gctttgcgaa tggccttgcg
     4021 gacagcgtcg cgatcgtggc cgtcgatctt ctgcacgtgc cagtgcgcgg cctcgaatcg
     4081 cttcttcaca tcctcggaca tcgagagacg cttgtcgccg tccagcgtga cgtcgttgtc
     4141 atcgtacagg tagatcagat tgcccaagcc ga
//
LOCUS       DATLVW010000255         6390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_380707, whole genome shotgun sequence.
ACCESSION   DATLVW010000255 DATLVW010000000
VERSION     DATLVW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380707"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..734
                     /locus_tag="VNN25_08050"
     CDS             132..734
                     /locus_tag="VNN25_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91517.1"
                     /translation="MLKDLWPNLAKGAAGFVLGLALWWGLSTPYARLLATVTEPLIRM
                     VERPAVTRLIAGGTDLTIDRDDFPRSSPRPGLTLMDLTANIVLLTTLFAVARRPISDR
                     NIVGFLLASVALVAVHIGAIIVNVESIYALRLGPWSQRNYGPFARNFWGAGAHFYSLI
                     GVFGAGFALWWLFRPSNEVPPDARKGDRRNTATKKRAPSR"
     gene            complement(771..1406)
                     /locus_tag="VNN25_08055"
     CDS             complement(771..1406)
                     /locus_tag="VNN25_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91518.1"
                     /translation="MFSTKILSRNMALAGAFTFLLGSSAFAQYGSYPGTYRAADRGYA
                     NRVVEGTVASVAVARYGERIRLTNGMDLVVPNSVTAVNQGRRYGAATLLPGDVVRMTV
                     YSREGDGRDARVLSFELLQSNSNSYFGNDRRMSGTIVSFDRRNRSMVVQTDRGRTVSV
                     DLSTYNGRMSWDTFRRGDRVSITGRMDRGNVFVEDIRVDNRDRDDQYDHRR"
     gene            1617..3035
                     /locus_tag="VNN25_08060"
     CDS             1617..3035
                     /locus_tag="VNN25_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXH91519.1"
                     /translation="MPEQKRATAMTDNANGIHLLFSGNSAREQMSVLASTLLSRRDAI
                     LEAWRAYGERVPGRNVASSLSRAQFNDHIPAVLDCLARTINSWPDEPDLETQQIENEK
                     VFNHGMQRWQQGYQLSELIREWGYLQISVAAELERYAAAHPGLDPAVMPAVRLAWAKL
                     CADGVTGSATQYGRLQQSESAGHVNDLETALTALHAMERSRAEAWRIAAHDLRGSVTV
                     VKGAATLLNAAGAEMPEPIRVEVADMLSKSVASLHEMLNDLLSLARLEAGHEQRDLST
                     FDAAALLRDFCTASQAAATDRGLFLKMDGPVSLPVEGDKPKVIRILQNLLLNAVKYTQ
                     RGGVSVTWGLDKTRDTDRWTFSVQDTGPGIDEAHAAPLAQELHEATEASNEAKATSND
                     RRRDMAPATTLPSESKALPPDQQPGEGVGLSIVKRLCELLDAGLELETGPNRGSTFRV
                     ILPRSYGDDSSATVPLAEDRST"
     gene            3250..>6390
                     /locus_tag="VNN25_08065"
     CDS             3250..>6390
                     /locus_tag="VNN25_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXH91520.1"
                     /translation="MAATRSRLKLPVQRGKQQQAPPAQDDAERSPSEDLLRVVGIGAS
                     AGGLDAFLDLLTAIPPDSGLAFVLVQHLDPRHDSMLVNILAGATTIPVQEITDGMHIE
                     RNHIYVIPPDAELTIQSNLLRLSPRMPKVPHRPMDTFLSSLAQDRTNRAIGVVLSGND
                     ADGAAGLQAIRDAGGITFAQSPETAKFDVMPRAAAAAADFILPPAGIAARLVSIARRE
                     GTRESAAHARPDAPEFERILQLLRATHPVDFAHFKSASIERRILRRVLLGNHEDLTSY
                     AESLEKDSAGVETLYQDLLIGVTSFFREPTRFEALKTIVFPAVVQNRHSEHSVRIWVA
                     GCSTGEEVYSLAITLLEFIDGDPNPPRISIYGTDINEQSLRKARAASYTDRALGGVSK
                     ERLAKFFTQVPGGYKISKAVRELCIFAAHDVTRDPPYSRIDLVTCCNVLIYFDPELQK
                     KAAALLQYALAPGGFLMLGSSENLRATNQLSAVSTKPLIYRKNQAPGALATFDIVPRS
                     LHPSIATASSAPSARRAAGVAHEEDDAFLAAHLAPAGVLINEQMEITRIRGEIAPFLA
                     LEPGEVSLNLFGLIRHHEVLAVLRPAVRKAFREKMTVSAEDILVTDDEGRRCISFNVI
                     PYGTSLPGHDRCWIVFHTVPEGATRKAESSVKRKEIDGLRHALAAAIDDREQLADEAA
                     AAAEEAQSSDEELRSTNEELETAKEELQSANEELSTLNDELRLRNTALVRINDDIENL
                     LGAVEIPILFVGSDLTVRRFNLTAGLLFNLRSEAIGRPLRTARSTLDVALLEKLAGAV
                     IETAAPADVEVQNSEGDWRMLRIRAYRTSDAKIDGAIVAVLDINVLKRSVLVAEEATR
                     AANMLSQASALLASSLDYETTLESLARLSTAAFADWCAVDLLNDDGSIRHLTVSHANP
                     VLRDLALQFQQVAFSEPENAPGAPQALRLRKSVLLTDIANSQESGIRPAAKVTQLIGA
                     LGLRSLISVPLIVRDKVLGTTTFSSSRRQYEPIDLQLAEELSQRAAIAIDTAMLFREA
                     ESANRYKDAFIGTVAHE"
BASE COUNT         1214 a         2094 c         1920 g         1162 t
ORIGIN      
        1 cgtccgcctc agctctctct tctggctcgg cgagcactac cggcaggtgt tcgacctttt
       61 ccacgttgcc gtctggcagt cgctcatcat tctcgccgcc atctcgatgt tcgttctctg
      121 gagctggaag tttgctgaaa gacctctggc caaatctcgc taaaggcgcg gccggtttcg
      181 tgctcgggct ggcgttgtgg tggggactgt caacgcccta cgcacggctg ctggccacgg
      241 tgaccgaacc gttgatccgg atggtggagc ggcccgcggt cacgcgcctg atcgccggcg
      301 gaacggacct gacgatcgac cgcgatgact tcccgcggtc atcaccgcgg cccggcctga
      361 cgctcatgga cctgacggca aacatcgtcc tgctcacgac gctctttgca gtggccaggc
      421 ggcccatcag tgaccgcaac atcgtgggct tcctgctggc aagcgtcgcg ctcgtcgcgg
      481 tgcacatcgg ggcgatcatt gtgaacgtcg agtcgattta tgcgttgcgg cttgggccat
      541 ggagccaacg aaactacgga ccattcgcgc ggaacttctg gggagcgggc gctcatttct
      601 acagcctcat cggcgtattc ggagcgggct tcgcactctg gtggctgttc cgcccatcca
      661 atgaagtgcc gccggatgcg agaaaaggcg acaggcgcaa tacagccaca aaaaagcgtg
      721 caccctcgcg gtgagggtgc acgctgacga tgattcaaaa cagctgagac ttacctgcgg
      781 tgatcgtact ggtcatcccg gtcgcgattg tcgacgcgga tgtcctcgac aaacacgttt
      841 ccacggtcca tgcgtccggt gatgctgacg cggtcgccgc ggcggaaggt atcccagctc
      901 atacggccgt tgtacgtgct gaggtcgaca gagacggtac ggcctcggtc tgtctgcacg
      961 accatcgaac ggttgcggcg gtcaaaggag acgatcgtcc cgctcatccg ccgatcgttg
     1021 ccgaagtacg aattggaatt cgactgcaga agctcgaacg agagaactcg cgcgtcgcgt
     1081 ccgtcgcctt cgcggctata gacggtcatg cggacgacgt cgcctggcag gagcgtggcc
     1141 gcgccgtagc gcctaccctg attgacggcc gtgaccgagt ttggaacgac cagatccata
     1201 ccattcgtca ggcggatgcg ctcaccgtaa cgagcgaccg cgacagatgc gacagtcccc
     1261 tcgacgaccc ggtttgcgta tccgcgatcg gcggcacgat atgtccccgg atagctacca
     1321 tattgcgcaa aagcggacga acccagcagg aacgtgaatg ctccggcgag cgccatatta
     1381 cgagacaaga tctttgtact gaacatttgg tgcctcccct ttaggtgttg ctcaccaact
     1441 gtccatcgca atagcggggc cactttcgcc tcatccgttg taatttcgct caaaagaacg
     1501 caacgagcgg tcgtgggcgc gttcttgcac tgatctaaag atatccgccc gaacgtagag
     1561 ctcctcgctc gatctgcgaa gcagacgtac cttccgggaa gaccggaagg agccgtatgc
     1621 ccgagcaaaa acgcgccacc gccatgaccg acaacgccaa cggcatccac ctgctgtttt
     1681 ccggcaattc tgcccgagag cagatgagcg tcctcgcctc gacactgctc agccggcgcg
     1741 acgcaatcct cgaagcatgg cgcgcgtacg gcgaacgggt gccgggccgt aacgttgcgt
     1801 cgtcactctc gcgtgcgcag tttaacgatc acattcccgc cgttcttgac tgtctggcgc
     1861 gaacgatcaa ttcgtggccg gatgagccag acctcgagac ccaacagatc gagaacgaga
     1921 aggtcttcaa tcacggcatg cagcgctggc agcagggata tcaattgagc gagctgattc
     1981 gtgaatgggg atatctgcag atcagtgtcg cggccgagct ggagcgctac gccgcggcgc
     2041 accccggtct cgatccagcc gtgatgcccg cggtgcgcct ggcttgggca aagttgtgtg
     2101 cggatggcgt caccggaagc gcgacgcagt acggccgcct gcaacagagc gaatcggccg
     2161 gacacgtcaa cgatctcgag accgcactga cggcgttgca tgcgatggag cgttcgcgtg
     2221 cggaggcgtg gcggattgca gcgcacgacc ttcgcggtag cgtgacggtc gtcaaaggcg
     2281 ccgcaaccct gctcaatgcg gccggagcag aaatgccgga gccgatacgg gtagaagttg
     2341 ccgacatgct cagcaagagc gtcgcgtcac tccacgagat gctgaacgat ctcctcagcc
     2401 tggcacgtct cgaagccgga cacgagcagc gcgacctctc gacatttgat gccgccgcgc
     2461 tgctgcggga tttctgcacg gcatcccagg ccgcggccac cgaccgaggg ctctttctga
     2521 agatggacgg gccggtctcc ctgccggtcg aaggcgacaa accaaaagtc attcgcattt
     2581 tgcagaacct gctgctcaac gctgtgaagt acacgcagcg cggcggcgtt tccgttacgt
     2641 gggggcttga taaaacgcgc gacaccgatc gctggacatt ctcggttcag gataccggtc
     2701 cgggcatcga cgaggcccac gccgcaccgc tggcgcaaga acttcatgaa gccacggaag
     2761 cgtccaacga agccaaagca acgagcaacg accggcggcg cgacatggct cccgccacta
     2821 ctcttccatc cgaatcaaag gcgctcccgc cggatcagca gccgggcgag ggagtcggtc
     2881 tgtcgatcgt caaacggctt tgtgaattgc tggacgccgg cctcgagttg gagaccggac
     2941 ccaaccgggg atcgacgttt cgcgtgatcc tgcctcgcag ttatggtgac gactcgagcg
     3001 caaccgtgcc gctcgccgag gataggagca cctagatgca tactcgcgcc ttgacccatt
     3061 acttggggaa ccgatcctcg tttcgtcatc acctcggaaa tgccggcgta tgctgaaccg
     3121 gttacctatt tacacgcctg atcagccggt cgcgacaggc gcgctcgtgt ttcccctgct
     3181 tttcatcaaa ccacctgcgc gttcgccact gcctccatcc tcagacggct atccctgaaa
     3241 ggatgcgaat tggcggctac tcgatcgcgg ctcaagctcc ctgtccaacg tgggaagcag
     3301 caacaggctc ctcccgcgca ggatgacgct gagcgttccc cgtccgagga cctcttgcgg
     3361 gtcgtcggca tcggcgcgtc tgcaggcgga ctcgatgcgt ttctcgatct gctgaccgcc
     3421 attcctcccg acagcgggct ggccttcgtc ctggtgcagc acctcgaccc gcgccacgac
     3481 agcatgctcg tcaacatcct ggctggcgcg accacgattc ctgtgcagga aatcacggac
     3541 gggatgcaca tcgagcgcaa tcacatctac gtcatcccgc ccgatgccga gctcacgatt
     3601 cagtccaatc ttctccggct gtctcctcgc atgccgaaag tcccgcatcg tccgatggac
     3661 acgttcttgt cctcgctggc gcaggaccgg acgaatcgcg ccatcggggt cgtgctttcc
     3721 ggcaatgacg cggacggcgc ggccggtctt caagccattc gcgatgccgg cggcattacc
     3781 tttgcgcaga gtccggagac ggcaaagttc gacgtgatgc ctcgagcagc ggccgcagcg
     3841 gcggacttta ttcttccgcc tgccggtatc gcggcccgcc tcgtgagcat cgcccgccgc
     3901 gagggaacgc gcgagagtgc ggcgcacgcc cggccggacg ctcccgaatt cgagcgcatc
     3961 ctccaactac tgcgcgccac gcacccggtc gacttcgcgc acttcaagag cgccagtatc
     4021 gagcgccgca tcctccgtcg cgtcctcctc ggcaaccatg aagacctgac cagttacgcg
     4081 gagtcgctcg agaaagacag cgcaggagtg gagacgctct atcaggacct gttgatcggc
     4141 gtcacgtcgt tcttccgcga gcccacgcgc ttcgaggcgc tgaagacgat cgtgtttccc
     4201 gctgtcgttc agaaccgcca ctcggagcac agcgtgcgca tctgggttgc aggatgctcg
     4261 accggcgaag aggtctactc cctcgccatc acgctgctcg agttcatcga tggcgatcca
     4321 aaccctccgc gaatctcgat ctacggcacg gacatcaacg agcagtcgct ccgcaaggct
     4381 cgggcggcgt cgtacacgga ccgggctctg ggcggcgtgt cgaaggagcg tctggcgaag
     4441 ttcttcacgc aagtgcccgg cggctacaaa atctcgaagg cggtgcgcga gctgtgtatc
     4501 ttcgccgcgc acgatgtcac ccgcgacccg ccgtactcgc ggatcgatct cgtgacctgc
     4561 tgcaatgttc tgatctactt cgatcccgag ctgcaaaaga aagcagcggc cctcctgcag
     4621 tacgcgctcg ctccgggcgg attcctgatg ctcgggagct ccgagaatct tcgcgccacc
     4681 aatcaactca gcgccgtgtc gacgaagccg ctgatttacc gcaagaacca ggcgccgggc
     4741 gcgctggcca ccttcgacat cgtgccccgc tctctccatc ccagcattgc cacggcatcc
     4801 tccgcgccgt ccgcgcgccg ggcggcgggc gtagctcacg aagaagatga tgccttcctc
     4861 gccgcgcatc tggcaccggc cggagtgctg atcaacgaac agatggagat cacgcgcatt
     4921 cgcggcgaga ttgctccgtt cctcgcgctg gagccgggcg aggtttcgct caacctcttc
     4981 ggcctgatcc gtcaccacga ggtactggcg gtcctgcggc cggccgtgcg caaggcattc
     5041 cgcgaaaaaa tgacggtcag cgcggaagac atcctcgtca cggatgacga aggccgccgt
     5101 tgcatttcgt tcaacgtgat tccgtatggc acttccctcc cgggccacga ccgttgctgg
     5161 atcgtgttcc acacggttcc ggagggggca acgcgcaaag cggaatcgtc cgtgaaacgg
     5221 aaagagatcg acggcttgcg ccatgcgctg gccgccgcca tcgacgatcg cgagcagctg
     5281 gccgatgagg ccgcggccgc cgccgaggaa gcgcaatcga gcgacgaaga gttgcgcagc
     5341 acgaacgagg agctggagac cgccaaggaa gagctgcagt cggcgaacga ggagctgagc
     5401 acactcaacg acgagctccg actccgcaac acggccctgg tgcggatcaa cgacgacatc
     5461 gagaacctcc tcggcgcggt cgagatccct atccttttcg tcggcagcga ccttacggtg
     5521 cgacgcttca accttacggc cggattgctg ttcaaccttc gttcggaggc tatcggccgg
     5581 ccgcttcgca ccgcgcgctc gacgctcgac gtggcgcttc tcgagaagct cgccggcgca
     5641 gtcattgaga ccgctgcgcc ggctgatgtg gaagtgcaga attcggaagg cgactggcgg
     5701 atgttgcgca tccgcgctta ccgcacctcg gacgcaaaga tcgacggcgc catcgtcgcc
     5761 gttctcgata tcaacgttct caagcgaagc gttctcgtcg ccgaggaggc cacgcgcgcc
     5821 gcgaacatgc tgtcgcaggc tagcgcgctg ctggcgtcat cgcttgatta cgaaaccacg
     5881 ctcgagtcgc tggcgcgcct gtccaccgca gcattcgccg actggtgcgc cgtcgacctg
     5941 ctcaacgatg acggctcgat ccgccacctc accgtgtcgc atgccaatcc cgtcctgcgc
     6001 gatctggcct tgcagttcca gcaggtagcg ttcagcgagc cggagaacgc gcccggcgct
     6061 ccccaggcac tccgcctgcg caaatcggtg ctgctgacgg atatcgccaa ctcgcaagag
     6121 agcggcatcc gtcccgcggc gaaagtcacg cagctcatcg gcgccctcgg gctgcgatcc
     6181 ctgatcagcg taccgctcat tgtccgcgac aaggttctcg gaacgaccac gttttcgtcg
     6241 tcgcgacgac agtacgagcc catcgacctg caactcgccg aggagttgtc gcaacgcgct
     6301 gcgatcgcca tcgacacggc catgctgttc cgggaagccg aaagcgccaa tcgatacaag
     6361 gacgcgttca tcggcaccgt cgcgcacgaa
//
LOCUS       DATLVW010000256        12441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_137747, whole genome shotgun sequence.
ACCESSION   DATLVW010000256 DATLVW010000000
VERSION     DATLVW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137747"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /gene="atpD"
                     /locus_tag="VNN25_08070"
     CDS             <1..761
                     /gene="atpD"
                     /locus_tag="VNN25_08070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXH91521.1"
                     /translation="GARLRVGLTGLTVAEHFRDAEGQDVLLFIDNIFRFTQAGSEVSA
                     LLGRMPSAVGYQPNLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATAFAH
                     LDATSVLSRQIAELGIYPAVDPLASTSKILDPRILGDEHYNTARAVQAVLQKYKDLQD
                     IIAIMGIDELSEEDRVIVNRARKIQRFLSQPFHVAEQFTGIKGQYVKIADTIKGFREI
                     VEGKHDDIPEQAFLLMGTIEQVLERAEQMKKEAA"
     gene            771..1193
                     /gene="atpC"
                     /locus_tag="VNN25_08075"
     CDS             771..1193
                     /gene="atpC"
                     /locus_tag="VNN25_08075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HXH91522.1"
                     /translation="MADALPTKLTLTVVTRERKIVEVDVDEVVLPASDGEIGVLPGHT
                     PLLTTLKIGEMRYRTGTVFHRMVLSWGFAEVLPARVIVLAERGILASEIDPAAAEAER
                     AAAEKELQYLSSHDPEFQLVEARLEESIAMIGIGREPH"
     gene            1195..1857
                     /locus_tag="VNN25_08080"
     CDS             1195..1857
                     /locus_tag="VNN25_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXH91523.1"
                     /translation="MDSWLESFAKTNFRRSSDTAHDLKTPLNVAVLNLELLRMRVSKL
                     AGGDDEKVNAYARSIELELRRMARIFDAFFVLSTPPKSDEELTTLDLCALCAEAAADA
                     SIELAAVDGPFPLSGHESRIRQAFTLFFEGAERVFRREDRQASVEKSAESFSLAIFGN
                     PAAEDFEMTKIFKFYYTDALGNPDLSLASARLIVETYGGELNAAEERDKVVIRLSFPG
                     KP"
     gene            1854..3233
                     /locus_tag="VNN25_08085"
     CDS             1854..3233
                     /locus_tag="VNN25_08085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH91524.1"
                     /translation="MKKVLIVDDDRATSAGMADVVEDWGYEAEVADTVKAGWSSISKM
                     VPDVAIVDLKLPDGSGLDLLHRIKETYPDVSVVILTGHATVDSAVKALKVGAEDYVTK
                     PVDLPRLQVILKTVEDKQQMKQEILELRRQLQKMGALGHLVGKSPRMQKLFEEIEMVA
                     NTDANVFIVGESGSGKEVVANTIHSLSRRRLKPFIAFNCGAISPTLIESEIFGHEKGA
                     FTNAIKRREGYFEVAKGGTVFLDEITEMPIELQVKLLRVLEEGKFRRVGGNEEIYIEA
                     RVIAASNRDPQKAIIDGKMREDLYYRLNVFPIEVPPLRERLEDIPLFSHFFLQKLNET
                     EEKKVETIETDFIEALQQYEWPGNVRELRNVINRAFIMARGSSLTLECLPDKLLGARK
                     RRSKEGVMIPLGQPMEEVERIVIEETLNMTDGDRRKTAEILGISYKTLYNKTKKYKAV
                     GGADEDEEE"
     gene            3437..5872
                     /gene="topA"
                     /locus_tag="VNN25_08090"
     CDS             3437..5872
                     /gene="topA"
                     /locus_tag="VNN25_08090"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXH91525.1"
                     /translation="MAKNLVIVESPAKAKTINKFIGSDYIVKASVGHVRDLPKSELGV
                     DEDTFEPTYEVLEGKEKVVSELRAAAKKATTVFIASDPDREGEAIGWHVMKLLGGDAT
                     KVRRILFHEITKNAVRKAIDQPGEIDMNKVNAQQARRVLDRLVGYKLSPLLWDKVRRG
                     LSAGRVQSVALKMIVDREESIKAFVPEEYWSFAAKLAAGMPPQFIAKLTKIDGKKADV
                     DNEEQARKIEAALKSGTFAVEAIARKEKKQTAAAPFITSTLQRTAYNRFKYPVKRTMQ
                     IAQKLYEGKELGAFGHVGLITYMRTDSVRISEDAITEVRQYIGTKYGADILPEKPNVY
                     RVKKAAQAQEAHEAVRPTSLEHEPDAIKDHLTREEYNLYKLIWDRFVASQMKPALFDV
                     TDVDIRNGAYTLRASGEVQKFAGFLAVFQDASVDGADDDDEKPENSKALPAMNEGEVL
                     SLVNLDTKQNFTQPPARYTEATLVKALEENGIGRPSTYGSIMTTIQTRDYTYKDDGKF
                     FPTQLGMLVTKLLKASFGDIIDEGYTAELEGKLDEIEEGKVEWHEAMRDFSLKFSKDL
                     ARAGKEMVEVKRTGVETDEICEKCGSPMAIKFGRFGEFLACSNYPECKNTKETARGDV
                     AETPEGEAIVCDKCGKPMQLKRSRFGQFYACTGYPDCKNTKDPRLLKANIPNEPQPPC
                     ENCGKEMVLKSGRYGPFYSCSGYPDCKTIRKIGGGKGTPPKPTGVSCPTCKEGELVER
                     RSRRGIFFSCSRYPKCDFALNNRPVPRPCPKCAAPYLLEKETKREGHIEYCNNPECDY
                     RAAIAARPNAANE"
     gene            6050..7570
                     /locus_tag="VNN25_08095"
     CDS             6050..7570
                     /locus_tag="VNN25_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HXH91526.1"
                     /translation="MTRFSSRTRRILGALSFFIATTAVAQPALVSDRVILRQVLQAMP
                     KGGDLHNHLSGSIYAESYLRFAAADGICVDSVKLTITQCATPDQLAADPAHASAIVPA
                     ATLFAPYSSLYGQMLDSLSMRQFRSGIESGHDHFFNTFGKFSPVSRAHVPEMLTEDVW
                     RLASENVDYVETIFAPDFGKARDLMKATTPGQSMADARAAIMQSGQIPPIIAAARAVI
                     DDAEAHMRTNLNCGTAKALPGCESTVRYLYELHRGVPLQQLFAEMVVGYELASADSRV
                     VGLNPVMAEDSYISMHDFDEQMRMFAFFHQLYPAIHLTTHAGELTPGLVPPEGLRNHI
                     RDSVVIAGAQRIGHGVDVSYEDDAAGLLSIMATRHVAVEVCLTSNDVILGVSGKAHPL
                     HLYLAAGVPVALATDDPGVSRDDMTNQYMRAVQDQGIDYPALKRLARNSLEYSFAEGD
                     SLWINHKYDEVAEPCAKDSGAAKTSETCSMFLAKNTKARIEWRLEQRLRQFEASWK"
     gene            complement(7559..8656)
                     /locus_tag="VNN25_08100"
     CDS             complement(7559..8656)
                     /locus_tag="VNN25_08100"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXH91527.1"
                     /translation="MPDSFIAAPPKDVLLDEGFRFVHGQTIAPFTIRYETFGELNADR
                     SNAILVCHALSASAHAAGRYRDDPDEKPGWWDGLIGYGKGIDLGKYFVICANLPGSCF
                     GTTAPSSIDPANGRAYGSRFPWPTIEDMVHSQKVLLDRLGITRLRAVAGGSLGGMQVL
                     MWAALYPELMDSIITMAAGAAVPVQGVAWHVIGRKMIESDARFNGGDYYDHPEPLRGL
                     QVARMVGHMTYLSAQALQSKFGRRRRGNTRQFEIDSYFEHQGRKFAAQYDANSYIRVQ
                     AAMDEMDLEEKYGSLQEAFAKWRGRTLLVSFDTDWLFPVGDVERVESAMAANGTAVQH
                     ERIASTNGHDTFLIDYGLITPPVTSFLASLP"
     gene            complement(8674..10302)
                     /locus_tag="VNN25_08105"
     CDS             complement(8674..10302)
                     /locus_tag="VNN25_08105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXH91528.1"
                     /translation="MLSDFCNTANAIAATTKKSEKERLLADYFVSLDDASLERAAVFF
                     SGSPFPRKDQRAAGVGGAALSDALVEVTGLATDEVWRSWSEYGDAGDTAAALFPVDPS
                     RSVTLSEAGETFDLLAATPGAIDKKNLLVTLLRKLDRDGVRYVVKILLREFRIGLQEG
                     LVESAIAKAFHRPLASVRRANMLTGDIGAAALLARADALDSAQMNLFHPFAFMLAQPE
                     EDPEEIVAMLGAGAFADDKYDGVRAQIHASAGEVRIYSRTLDDVTHRFPEIESAGRAL
                     GREAVLDGEIVAFSDRVLPFATIQKRLGRRKVSDALLRDAPVVFFAFDLLFIDGEVLL
                     ETPLRDRLARLRELLSSGPASIRFTNQTEVREAAHVDELFDAARARANEGLVVKAPDS
                     VYTPGRRGKSWLKYKKALATLDCVVTAAEYGHGKRRSVLSDVTFAVRRDNELVNIGKA
                     YSGLTDAEIEMLTKHFQATTTSRHGPVHVVEPTIVLEIAFDRIQESARHKSGFALRFP
                     RIAHLRDDKTVDQISTIDEVRRIYEGQLKRERQE"
     gene            10468..11400
                     /gene="mdh"
                     /locus_tag="VNN25_08110"
     CDS             10468..11400
                     /gene="mdh"
                     /locus_tag="VNN25_08110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXH91529.1"
                     /translation="MKTKVTVVGGGNVGATVAQGIALKELADVIVVDIVEGVPQGKTL
                     DLMETAPVENYDSILRGSNSYDETAGSDVVVITAGLPRKPGMSRDDLLWKNEEIVAGV
                     TREVARRSPNSILVIVSNPLDAMCEVARRVSGFPRERVIGMAGVLDSARMRAFIAMEL
                     NVSVENTHAFVLGGHGDTMVPLPRYSTVAGIPITELISKERIDAIVERTRTGGAEIVN
                     LLKTSAWYAPGAATVEMVEAILKDKRKILPCAAYLTGEYGFNNLYVGVPVKLGRNGIE
                     QIIEITLTPDEKAALQKSADAVRELFDTLKTTAA"
     gene            complement(11397..11585)
                     /locus_tag="VNN25_08115"
     CDS             complement(11397..11585)
                     /locus_tag="VNN25_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91530.1"
                     /translation="MLEKPQKISQKTGDAFMATLEKAGAKKTWVAKRDMLVFVADQLT
                     VELLRVNDGFIVRMHPND"
     gene            complement(11633..12022)
                     /locus_tag="VNN25_08120"
     CDS             complement(11633..12022)
                     /locus_tag="VNN25_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91531.1"
                     /translation="MRKKDIKQLVVEVPVALAEELEKYCAKHSKFKGGVIREAIETLL
                     EHEGDSTSRFIVTLPESTSEDLAVFRAVRDEATREGVVRSAIREYIDHVLELEPHTSV
                     RFRGEKQKVQATRARSNVRAISGNKSR"
     gene            12131..>12441
                     /locus_tag="VNN25_08125"
     CDS             12131..>12441
                     /locus_tag="VNN25_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91532.1"
                     /translation="MKKLVIDIETVGTPWEELDAYVREYLIKGLNDGDAEETRRAGGL
                     SPFRGRIIAIGVINIEDGRSCAMYEVPGQTQLAVEKSGSRTYMSGSEKQILEKFWEFF
                     D"
BASE COUNT         2583 a         3887 c         3679 g         2292 t
ORIGIN      
        1 cgggagcgcg cctccgcgtc ggtctcaccg gcctgacggt cgccgagcac ttccgcgatg
       61 ccgaagggca ggacgtgctc ctgtttatcg acaatatctt ccgcttcacc caggcaggat
      121 cagaagtttc ggccctcctc ggccgcatgc ccagcgcggt cggctatcag ccgaatctgg
      181 cgactgaaat gggcgagttg caggagcgca tcacatccac caagcgtggc tcgatcactt
      241 cggtccaggc gatctacgtt cccgcggacg atctcaccga tcccgcaccg gcaacggcct
      301 tcgctcacct cgacgcgacg tcggttctct cgcgccagat cgccgagctt ggtatctacc
      361 cggccgtcga tccgcttgcc tcgacctcca aaattctcga cccgcgcatc ctcggcgacg
      421 agcactacaa caccgcccgc gccgtccagg ccgtgctgca gaagtacaag gatctgcagg
      481 acatcatcgc catcatgggt atcgacgagt tgtcggaaga ggatcgcgtc atcgtcaacc
      541 gggcccgcaa gattcagcgc ttcctctcgc agcccttcca cgttgccgag cagttcaccg
      601 gcatcaaggg gcagtacgtc aagatcgccg acacgatcaa aggcttccgc gagatcgtcg
      661 agggcaagca cgacgacatt ccggagcagg ccttcctgct catgggaacg atcgagcagg
      721 tgctcgagcg cgccgaacag atgaagaagg aagcggcgta aaggaatcga atggcagacg
      781 cgctcccaac caagctcact ctcaccgtcg tcacgcgcga acggaagatc gtcgaagtcg
      841 acgtcgatga agtcgtgctg ccggcgagcg acggtgagat cggcgtgctg cccgggcata
      901 cgccgctgct gacgacgctg aagatcggtg agatgcgtta tcgcaccgga accgtgttcc
      961 accggatggt gctttcgtgg ggcttcgccg aggtgttgcc tgcccgcgtc attgtgctgg
     1021 cggaacgtgg cattctcgcc tcggaaatcg atcccgcggc agccgaggcc gagcgcgcgg
     1081 cggcggaaaa ggaactgcag tacctttcgt cccacgatcc ggaattccag ctcgtcgagg
     1141 ctcgtcttga agaatcgatc gcgatgatcg ggattggccg cgaaccccac tgacatggac
     1201 tcctggctcg agagcttcgc caaaacgaac tttcgacgta gcagcgacac ggcgcacgat
     1261 ctcaagacgc cgttgaacgt cgccgtgctc aacctcgagt tgctccgcat gcgcgtctcg
     1321 aagcttgcgg gaggcgacga cgagaaggtc aacgcgtacg ctcgatcgat cgaactggaa
     1381 ctccgccgca tggcgcgaat cttcgacgcc ttcttcgtcc tttccactcc tccgaaaagc
     1441 gacgaggagc tgacgacgct cgatctctgc gcgctttgcg ccgaggcggc ggcggacgcg
     1501 tcaattgagc tggctgccgt cgatgggccg tttcctctat ctggtcatga atccagaatt
     1561 cgacaagcct tcacgttgtt ttttgaggga gccgagcggg tctttcgtcg agaggaccgg
     1621 caggcatcgg tcgagaagag tgcagaatcg ttctctctcg ccattttcgg aaatccggca
     1681 gccgaggact tcgaaatgac gaagatcttc aagttctact acaccgacgc gctcggtaac
     1741 cccgatctct cgctggcatc tgccagactc atcgtggaga cctacggcgg tgagctgaac
     1801 gctgccgagg aacgtgataa ggtcgtgatc cgtctgtcct tcccgggtaa accatgaaaa
     1861 aagtattgat cgttgatgat gatcgcgcca cgagtgccgg aatggccgac gtggtcgaag
     1921 actggggtta cgaggcggag gtcgccgaca ctgtcaaagc cggctggagc tccatttcga
     1981 agatggtccc cgacgtcgcg atcgttgacc tgaaactccc cgacggctcc ggtctcgacc
     2041 ttctgcaccg gatcaaagaa acctatcccg acgtctctgt cgtcatcctc acgggccacg
     2101 ccaccgtcga ttccgcggtc aaagcgctca aggtgggcgc cgaagactac gtcacgaagc
     2161 cggtcgacct cccgcgcttg caggtcatcc tcaagaccgt cgaagacaag cagcagatga
     2221 agcaggagat cctcgagctt cgtcgtcagc tccagaagat gggagcgctg ggacatctcg
     2281 tcggcaagtc gccgaggatg cagaagctct tcgaagagat cgagatggtg gcgaacaccg
     2341 acgcgaacgt cttcatcgtc ggcgagtctg gctcgggcaa ggaagtcgtt gccaacacga
     2401 ttcactctct ctcgcgccgg cggctcaagc cgttcatcgc cttcaactgc ggcgccatct
     2461 cgccgacgct catcgagtcc gagatcttcg gtcacgaaaa aggcgcattc acgaacgcga
     2521 tcaagcggcg cgaaggctac ttcgaggtcg ccaaaggcgg cacggtcttt ctcgacgaga
     2581 tcaccgagat gccgatcgag ctgcaggtga agctcctgcg cgtgctcgag gaaggaaagt
     2641 tccggcgcgt cggcggcaac gaagagattt acatcgaggc gcgcgtcatc gccgcatcca
     2701 accgcgatcc gcaaaaggcg atcatcgacg gcaagatgcg cgaggatctt tactaccgcc
     2761 tcaacgtctt ccccatcgaa gtgccgccgc ttcgcgagcg cctcgaagac atccccctgt
     2821 tctcacactt cttcctgcag aagctgaacg agaccgaaga gaagaaggtg gagacgatcg
     2881 aaacggactt catcgaagcg ctgcagcagt acgagtggcc aggcaacgtt cgggagctgc
     2941 gcaacgtgat caaccgcgcc ttcatcatgg ctcgcggcag cagcctgacc ctcgaatgtc
     3001 ttcctgacaa gctcctcggc gcccgcaagc gccgttctaa ggaaggcgtg atgattcccc
     3061 tgggccagcc gatggaagaa gtcgagcgga tcgtcattga agagacgctg aacatgaccg
     3121 atggcgaccg ccgcaagacc gccgagattc tgggcatctc gtacaagacg ctctacaaca
     3181 agacgaagaa gtacaaagcc gtcggtggcg ccgacgagga cgaagaagag taggccgata
     3241 cgctcgacgg cgttagttgg ccacgccaca tcgaccattt ggcgccgaaa ctccacgtct
     3301 cgtagcggtt tttggccttc cagacctccg gtgctatcgt ccgcgcctca gaccactccg
     3361 ccgcctcacg tacgcgtgcg catgatgcgc gcgcgaggaa ttccggcgtc ggcgtggaag
     3421 tttggggcgt tccgcaatgg ccaaaaacct cgtcatcgtc gagtctcccg caaaagcgaa
     3481 gacgatcaac aagttcatcg gtagcgacta catcgtcaaa gcctcggtcg gtcacgtccg
     3541 cgatctgccc aagagcgagc taggtgtcga cgaagatact ttcgagccga cgtacgaagt
     3601 tctcgaagga aaagagaagg tcgtcagcga gctgcgtgca gccgcaaaga aagccacgac
     3661 cgtcttcatc gcgtcggacc ctgatcgcga gggagaggcg atcggctggc acgtgatgaa
     3721 gctactcggc ggcgatgcca cgaaagttcg ccgcatcctt ttccacgaaa tcacgaagaa
     3781 cgcggtccgc aaggcaatcg atcagccggg cgagatcgac atgaacaagg tcaatgcgca
     3841 gcaggcccgg cgcgtcctcg accgtctggt cggctacaag ctctctcccc tgctctggga
     3901 caaagtgcgg cgcggacttt ctgccggccg cgtgcaatcg gttgcactaa agatgatcgt
     3961 cgaccgcgaa gagtcgatca aagcgttcgt ccccgaggag tattggagct tcgcggcgaa
     4021 actggccgcc ggcatgccgc cgcagttcat cgccaagctg acgaagatcg acgggaagaa
     4081 agcagacgtc gacaacgagg aacaggcccg caagatcgaa gcagctctga agtcgggtac
     4141 gtttgcagtc gaagcgattg cgcgaaaaga gaagaagcag accgcggcgg cgccgttcat
     4201 cacgtcaacg ctgcagcgca cagcgtacaa ccgcttcaaa tatcccgtga agcgcacgat
     4261 gcagattgcg cagaagctct acgaaggcaa agagcttggg gcgttcgggc atgtcggcct
     4321 gatcacctac atgcgtaccg actcggtccg catcagcgaa gatgccatca cggaagtgag
     4381 acaatatatc ggcacaaaat acggcgctga tatccttcca gaaaagccaa acgtttatcg
     4441 tgtcaagaaa gctgcacagg cgcaagaggc ccacgaggcc gtccgtccga catcgctcga
     4501 acacgagccc gacgcgatca aggatcacct cacccgcgag gagtacaacc tctacaagct
     4561 gatctgggac cgcttcgtag cctctcagat gaagcccgct ctcttcgacg tcaccgatgt
     4621 cgatatccgc aacggtgcgt acacgctccg cgcttccggt gaagtgcaga agtttgccgg
     4681 cttcctggcc gtcttccagg atgcatcggt cgatggcgcg gatgacgacg acgagaagcc
     4741 ggagaactcc aaagcgctcc cggcgatgaa cgagggcgaa gtgctctcgc tcgtgaatct
     4801 cgacacgaaa cagaacttca cacagccccc cgcccgctac acggaagcaa cgctcgtgaa
     4861 ggctctggaa gagaacggca tcggccgtcc gtcgacgtac ggctcgatca tgaccacgat
     4921 ccagacgcgc gactacacct acaaggatga cggcaagttc tttccgacgc agctcggcat
     4981 gctcgtcacg aaactgctca aagcttcctt cggcgacatc atcgacgaag gttacacggc
     5041 ggagctggag gggaagctcg acgagatcga agagggcaag gtcgagtggc acgaggcgat
     5101 gagagacttc tcgctgaagt tcagcaagga tctcgcccgc gccggcaaag agatggtcga
     5161 ggtgaagcgc accggcgtcg agaccgacga gatctgcgag aaatgcggct ctccgatggc
     5221 catcaagttc ggccgcttcg gcgagttcct ggcgtgctcg aactatcctg agtgcaagaa
     5281 cacgaaagaa acggcgcgcg gcgatgtggc ggagacaccc gagggcgagg cgatcgtctg
     5341 cgacaagtgc ggcaagccga tgcagctcaa gcgctcgcgc tttgggcaat tctatgcctg
     5401 caccggttat ccggactgca agaacaccaa agacccgcgt ttgctcaaag cgaatatccc
     5461 gaacgagccg cagccgcctt gcgagaactg cggcaaggag atggttctca agagcggccg
     5521 ttacggcccg ttctactcct gctccggcta tccggactgc aagaccatcc gcaagatcgg
     5581 cggcgggaaa ggcacacctc cgaagccgac cggcgtctcc tgccccacct gcaaggaagg
     5641 cgagctggtc gagcgccgtt cgcggcgcgg catcttcttc tcgtgctcac gctatcccaa
     5701 gtgcgacttc gccctgaaca atcgcccggt accgcgccct tgcccgaaat gcgccgcccc
     5761 ctatctcctc gagaaggaaa cgaagcgcga gggccacatc gagtattgca acaaccccga
     5821 gtgcgattat cgggcggcga tcgccgcaag gccgaacgca gcgaacgaat agcagtcgct
     5881 ttaatccaca cgcatttgct gtgtgttatc gcgcgccatt agccgcacat tagcggttaa
     5941 tattcacggc gatttcgcga tcgcgatttc gtgagcattt tctgcacgta actgatacct
     6001 attaacgtgc tgtcctgccg cgctacactc cgctcacgga ggtccttcca tgacgcgctt
     6061 ttcctctcga acacgccgaa tcctcggcgc gctgtccttc ttcatcgcca cgactgcggt
     6121 ggcgcaacca gcgctggtct ccgaccgcgt catcctgcgt caggttctcc aggcgatgcc
     6181 caaagggggc gacctgcaca atcacctctc cggctccatc tacgccgagt cgtatctccg
     6241 attcgcggcc gcggacggga tctgcgtcga ttccgtcaag ctgaccatta cgcagtgcgc
     6301 tacgcccgac caactcgctg ctgatcctgc ccatgcctcg gccatcgtgc ccgcggccac
     6361 tctgttcgca ccgtactcct cgctctacgg acagatgctc gattcacttt cgatgcggca
     6421 attccggtcg gggatcgagt cggggcacga tcacttcttc aacacgttcg gcaagttcag
     6481 cccggtctct cgcgcacacg ttccggagat gctgactgag gatgtctggc gcctggccag
     6541 cgagaacgtc gactacgtcg aaacgatctt cgcgcccgac ttcggcaaag cacgcgacct
     6601 gatgaaggcc actactccgg gccagagcat ggcggacgcg cgcgctgcaa tcatgcagag
     6661 cggacagatt ccgccgatca tcgccgccgc ccgggctgtg atcgacgacg ccgaggcgca
     6721 catgcgcacg aatctcaact gcggaacggc gaaggcgctg ccgggttgcg agtcgaccgt
     6781 tcgctatctg tacgagttgc accgtggcgt tccgctgcag cagctcttcg cggagatggt
     6841 tgtcggctac gaactggcca gcgctgactc gcgcgttgtc gggctcaatc ctgtgatggc
     6901 cgaggatagc tacatctcga tgcacgactt cgacgaacag atgcgcatgt tcgcgttttt
     6961 tcatcagctc tacccggcta ttcacctcac gacgcacgcg ggcgagctca cgccgggtct
     7021 ggtgccgcct gaagggcttc gcaaccacat tcgcgactcg gtcgtgatcg ccggcgcgca
     7081 gcggatcggc catggagtcg atgtctcgta cgaagacgat gctgccggac tgctgtcgat
     7141 catggccacc cgtcatgtcg ccgtcgaagt ctgcctcacc agcaacgacg ttatcctggg
     7201 tgtttcaggc aaagcgcatc cgctccatct ttacctcgca gcgggggttc cggtcgcact
     7261 cgcgacggac gatccgggcg tctcacgcga tgacatgacg aatcagtaca tgcgcgccgt
     7321 gcaggaccag ggtatcgact acccagcgct caagcggctg gcgcgcaaca gcctcgagta
     7381 ctcgttcgcc gagggtgact cgctctggat caatcacaag tacgacgaag tcgccgagcc
     7441 gtgtgcaaaa gacagcggcg cagcgaagac atcggaaacg tgctccatgt ttcttgcgaa
     7501 aaacacgaaa gcgcgcatcg agtggaggct ggaacagcgc ctccgccagt tcgaagcgtc
     7561 atggaagtga cgccaggaac gaggttactg gaggcgtgat caggccgtag tcgatgagga
     7621 aggtgtcatg gccgttcgtc gatgcgatcc tctcgtgttg gacagcggtg ccattggccg
     7681 ccatcgctga ctcgacgcgc tcgacgtcgc cgaccggaaa gagccagtcg gtgtcgaacg
     7741 agacgagaag cgtgcggcca cgccacttgg caaaggcttc ctgtaacgaa ccgtacttct
     7801 cttcgagatc catctcatcc atcgctgcct gaacgcggat atatgagttg gcgtcgtact
     7861 gcgcggcgaa cttccgtccc tgatgctcga agtaggagtc gatctcgaac tgccgcgtgt
     7921 tgccgcgccg gcgcctgccg aatttcgact gcagcgcctg ggcggaaaga taggtcatgt
     7981 ggcccaccat tcgcgcaacc tgcaacccgc gcagcggttc gggatgatcg tagtaatcgc
     8041 cgccgttgaa gcgggcgtcg ctctcgatca tcttgcgccc gatcacgtgc cacgccactc
     8101 cctgcacggg gaccgccgcg cccgcggcca tggtgatgat cgaatccatc agctcgggat
     8161 agagagcggc ccacatcaga acctgcattc cgccaagcga tccgccggcg acagcacgaa
     8221 ggcgtgtgat gcccagccgg tcgagaagaa ccttctgcga atgcaccatg tcctcgatcg
     8281 tcggccaggg gaaccgcgat ccatacgctc gcccattcgc cgggtcgatg gacgatggtg
     8341 ccgtcgttcc gaaacaggac ccgggaaggt tcgcgcagat gacgaagtac ttcccgaggt
     8401 cgatcccctt cccgtatccg atgaggccat cccaccatcc cggcttctca tcgggatcat
     8461 cgcggtatct gccggcggca tgcgcgctgg ccgagagcgc atggcagacg agaatggcat
     8521 tcgatcgatc ggcattcagc tcgccgaacg tttcgtagcg gatggtgaag ggagcgatgg
     8581 tctggccgtg gacgaagcgg aacccttcgt ccagcaggac atccttcggc ggcgcggcga
     8641 tgaacgagtc gggcatcagg accgcgaagg atctcactcc tgacgctcgc gcttcaactg
     8701 tccttcgtaa atccggcgta cctcatcgat cgtggagatc tgatcgaccg ttttgtcgtc
     8761 gcgaagatgg gcaatgcgag ggaatcgaag ggcgaagccg gacttgtgac gcgccgactc
     8821 ctgaatccga tcaaacgcga tctcgaggac gatggtcggc tcgaccacgt gcaccggtcc
     8881 atgcctcgat gtcgtcgtag cctggaagtg cttggtcagc atctcgattt ccgcatcggt
     8941 aaggcccgag tacgccttgc cgatgttgac cagctcattg tcgcggcgaa cggcgaaggt
     9001 gacatcggac agcaccgagc ggcgcttgcc gtgcccgtac tccgcggcgg tgacgacgca
     9061 atcgagcgtc gccagcgcct tcttgtattt cagccacgac ttgccgcggc ggccgggcgt
     9121 gtaaaccgaa tccggagctt tgacgacgag tccttcgttt gctcgtgcgc gtgccgcgtc
     9181 aaacagttcg tcgacgtggg cggcttcgcg aacctctgtt tgattagtga accgaatcga
     9241 cgcgggccca ctgctgagaa gctcgcgcaa gcgggcgagc cgatcgcgca gcggcgtttc
     9301 aagaagaact tcgccgtcga tgaagagcag atcgaacgca aagaagacga ccggtgcatc
     9361 gcgcaacaga gcgtcggaga ctttgcgccg cccgagccgt ttctgaatcg tggcgaatgg
     9421 cagaacgcga tcggagaacg ccacgatctc gccgtccaga acagcctccc ggccgagagc
     9481 acgccctgcc gactcgatct ccgggaaacg atgcgtcaca tcgtcgagcg tccgcgagta
     9541 gatccgcact tcgccggcac tggcgtgaat ctgagcacgg acgccgtcgt acttgtcgtc
     9601 cgcgaaggct cccgcgccga gcatggccac gatctcttcg ggatcctcct ccggctgggc
     9661 gagcatgaaa gcaaacgggt ggaacagatt catctgcgcg ctgtcgagcg catctgcgcg
     9721 cgccagaagc gcggcggcgc cgatgtcccc ggtaagcatg ttggcccgcc tgaccgaggc
     9781 cagcggacga tggaatgcct tggcgatcgc ggattccacg agcccttcct ggagtccgat
     9841 gcgaaactcg cgaaggagga tcttcacgac gtagcgaacg ccgtcccggt caagctttcg
     9901 aagcagcgtc acgaggaggt tcttcttgtc gatggcgccc ggcgtcgctg ccagcaaatc
     9961 gaacgtctcg cctgcttccg atagggtcac ggaacggctc ggatcgacag gaaacaacgc
    10021 cgctgccgtg tcgccagcat caccgtattc cgaccaggaa cgccagacct catccgtcgc
    10081 caggccagtc acctcgacga gcgcatcgct cagtgccgcg ccgccgactc ctgcggctcg
    10141 ctggtccttg cgcggaaacg gcgagcccga aaagaaaacg gcggcccgct cgagtgatgc
    10201 gtcatcgagc gagacgaagt aatcggcaag gagacgttcc ttctccgact tcttcgtcgt
    10261 cgcggcaatg gcgttggcag tgttgcagaa gtcggaaagc atgtcttgaa ttggagtcga
    10321 ttagcggtcg ggggtcggta gtgtgacagc cgacaccgat ctctactggc gaatctctac
    10381 actcactgcc accgggacct cgcggcctcg caaccgcgaa ccgcgatata tgatcccgcc
    10441 gcaaatatcg cacccccgag gtatgagatg aaaacgaaag ttacagtggt cggcggcggc
    10501 aatgtcggtg ccacagttgc gcagggaatc gcactcaaag agctcgccga tgtcatcgtc
    10561 gtcgacatcg tcgaaggcgt cccccagggg aaaaccctcg acttgatgga gaccgcgccc
    10621 gtcgagaact acgactccat cctccgcggc tcgaacagct acgacgagac cgccggctcc
    10681 gacgtcgtcg tgatcacggc cgggttgccg cgcaagccgg gcatgtcgcg cgatgacctg
    10741 ctctggaaga acgaagagat cgtggccggt gtcacccgcg aggttgccag gcgctcgccc
    10801 aacagcatcc tggtaatcgt ttcgaacccg ctcgacgcga tgtgcgaagt tgcccggcgc
    10861 gtctccggct ttccccgcga gcgtgtgatc ggcatggccg gcgttctcga ctccgcacgc
    10921 atgcgcgcct tcatcgccat ggaactgaac gtctccgtcg aaaataccca cgccttcgtc
    10981 ctcggcggac acggcgacac gatggttccg ctgccgcgct actcgacggt cgccggcatc
    11041 cccatcactg agctgatctc gaaagagcgc atcgacgcga tcgtcgagcg cacacgcaca
    11101 ggtggcgcgg agatcgtcaa cctgctgaag acctcggcct ggtacgcgcc aggcgcagca
    11161 acggtcgaga tggtcgaagc gatcctcaaa gacaagcgca agatcctgcc gtgcgcggcc
    11221 tatctcaccg gcgagtacgg attcaacaac ctctacgtcg gcgtgccggt gaagcttggc
    11281 cggaacggta tcgagcagat catcgagatc acgctgacgc cggacgagaa ggccgcgctg
    11341 cagaaatcgg cggatgcggt gcgggagttg ttcgatacgc tgaagacaac cgcagcctag
    11401 tcgttcggat gcatgcgcac gatgaaccca tcgttcactc gcagcaactc gaccgtcagc
    11461 tgatcggcga cgaagacgag catgtctctc ttcgcgaccc acgtcttctt ggcgcccgct
    11521 ttttcgagcg tggccatgaa agcgtctccg gtcttctggc tgattttctg cggcttctct
    11581 aacatgatga atctcctaac tccttttatc ctctccgccc gctcccccaa acctatcgag
    11641 atttgtttcc gctgatcgct cggacgttgc tacgcgcacg cgtcgcctgc actttctgtt
    11701 tttcgcctct gaagcgcacg ctggtgtgtg gctccagctc cagcacgtga tcgatgtatt
    11761 cgcggatcgc gctcctcacc actccctcgc gcgtcgcttc gtcgcgcaca gcgcgaaaca
    11821 ctgcgaggtc ttctgatgtt gactcaggta acgtgacgat gaatcgagaa gtgctgtccc
    11881 cctcatgttc gagaagcgtt tcgatcgcct cgcggatcac gccgcctttg aacttggagt
    11941 gcttcgcgca gtacttttcg agctcctccg cgagggctac aggcacctcg accaccaatt
    12001 gcttgatgtc cttctttcgc acggtaacag gcgattatag atttttctag aaaatgtgca
    12061 accgatgttg ggcagtccat gtttcccagt tcgctgcgcg cttcttctca gctacgattg
    12121 gttacccccc atgaaaaaac ttgtcattga tatcgaaacg gttggaacgc cgtgggaaga
    12181 gctcgacgcc tacgttcgcg agtacctcat caaagggctc aacgatggcg acgccgagga
    12241 gacgcggcgt gctggcgggc tctcgccgtt tcgcggacgc atcatcgcca tcggcgtaat
    12301 caacatcgaa gacggtcgct cctgcgcgat gtacgaagtc cccggacaaa cgcagctcgc
    12361 agtcgagaaa agcggttcgc ggacgtacat gtccggcagt gagaagcaga tcctggagaa
    12421 attctgggag ttcttcgaca g
//
LOCUS       DATLVW010000257         9995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_92593, whole genome shotgun sequence.
ACCESSION   DATLVW010000257 DATLVW010000000
VERSION     DATLVW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92593"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VNN25_08130"
     CDS             <1..186
                     /locus_tag="VNN25_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXH91533.1"
                     /translation="LDTDTTDSMDSETWILHQNHFQIEAMANLDKVPPAGAVIVVTWP
                     KVENGVGFPARAFAILP"
     gene            complement(295..945)
                     /locus_tag="VNN25_08135"
     CDS             complement(295..945)
                     /locus_tag="VNN25_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXH91534.1"
                     /translation="MSRLRVLSFSISIDGFGAGPNQDLENPLGVNGPEMFDWFFHTRT
                     WQQMHGGEGGESGVDDDMASQSFDGIGAWILGRNMFGPVRGPWPDDEWKGWWGEEPPY
                     HVPVFVLTHHPRAPLAMKGGTEFRFVTGGIEEALQQARDAAQGKDVRLGGGVSTIRQY
                     LQAGLIDELHLVISPVLLGRGEHLLHGIDLRALGYECTKHVSGGRAAAHVILRKRT"
     gene            1087..1377
                     /locus_tag="VNN25_08140"
     CDS             1087..1377
                     /locus_tag="VNN25_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HXH91535.1"
                     /translation="MVLFDSILAWLVSVVGVATGAAYIPQALRIWKRRSSDDVSILTY
                     LLFLGGQIVYLVYGIRIRQWPLIIGMAANIVGSLAVIGSALRFRAGRGGPKE"
     gene            complement(1720..3087)
                     /locus_tag="VNN25_08145"
     CDS             complement(1720..3087)
                     /locus_tag="VNN25_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXH91536.1"
                     /translation="MTIVELSRLLDSGKVTSRQLVEQSLAAIKDPQGEGARTFLSVHE
                     NEALAAADRVDAQRRGGARLPVLAGIPISIKDLFDEAGVVTLGGSKVLVGSPPATRDA
                     TVVERLRNAGAVIIGRTNLTEFAFSGLGINPHYGTPRNVFDRAAGRIPGGSSSGAAIS
                     VTDGMAAGAIGTDTGGSVRIPAALNGLVGFKPTARRVPREGVLPLSFTLDSIGPIAKC
                     VADCALLDHVLAAETDSVPTATHLRGLRFAVPKTVFQDDLSPAVARAFTAALDRLSAA
                     GAIIIELPMIEFTQAAVVNPRAALTSAEAFSLHRQWMKEKADQYDPRVIARIRPGETI
                     TAADYIELLQLRERFIKSINAAASGYDAMLMPTTPDTAPTITEASKDDETYSRLNGRM
                     LRNPSVVNLFDGCALSLPCHDPGSAPVGLMIAGTQNTDRRILAIGLAVEEVVWKGRSL
                     STRHD"
     gene            complement(3211..3390)
                     /locus_tag="VNN25_08150"
     CDS             complement(3211..3390)
                     /locus_tag="VNN25_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91537.1"
                     /translation="MPPSLKTGEGAEGYFESKMVGRNGHSSLIAWPLSDTMSPAAEAV
                     ISGRLAGTDRTFDKE"
     gene            3400..4098
                     /locus_tag="VNN25_08155"
     CDS             3400..4098
                     /locus_tag="VNN25_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HXH91538.1"
                     /translation="MIKHIAVAIALSLAPVAFAQMYIVPEGDCGAVTLHVTRGTDFPN
                     LGEAISADRVKDAHVVRDRPIERVIVTPVAGPRSLDFSANVPDREGVVMAAVDLKPVV
                     SGNETRTEHAKAFLFCGATTPRADWQLEAGLGLEIIPQWNGIRPKMKQGDEMQFIVVH
                     KTTGHPIQDVPVELYGAGEGRIGAGVPNQSGGVSFPYPKPGRYMVTTTYRRPDPQQAE
                     HWLVDTSTLTFEIK"
     gene            complement(4109..4936)
                     /locus_tag="VNN25_08160"
     CDS             complement(4109..4936)
                     /locus_tag="VNN25_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HXH91539.1"
                     /translation="MKRRCHTAFVVAALLILSALVPAQGDDLSWQLIQPGTELASIAV
                     KGPSDPGNRLYVVRVDPARAKLTVALASETKTAARTAAEWCRTSNLAVAINLGMFQND
                     GQSNVGYLRHGSHVNNPRWNDYNSILALNSSGASDPRVLWLDRDQTKDVQQLARYEIV
                     VQNLRLIARGRRNVWAKSDKRWSEAAVAIDSQGRVLFLFSRAPYTMREFNDLLLKLPL
                     AIVAAMHVEGGPEASLSIHAPGIDLDLAGSYETGFWPDDSNHKQWPLPNVLGVVREK"
     gene            5200..5586
                     /locus_tag="VNN25_08165"
     CDS             5200..5586
                     /locus_tag="VNN25_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91540.1"
                     /translation="MRLRDAAADDARVHTLRRNLFEFAEVQATKPKVSIISQGSPAVV
                     PVTVAVLETPVVASPPPLVFGYRFLGTFGPAASLIAAFAGNGEVLTAREGDHIGQSFV
                     LRRIGTRDVEVAALGQQATLRVNIDP"
     gene            complement(5906..6796)
                     /locus_tag="VNN25_08170"
     CDS             complement(5906..6796)
                     /locus_tag="VNN25_08170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH91541.1"
                     /translation="MRKVLSALVDLSAGLLFVLVAFFDGRVLLQSPHLVYLCFPVLIT
                     VAVAAGYWRGSRNALPPWVTAVLTNIPLLLFALAFFSGRNKPFILFPVLTFVSVSAGI
                     AMARLRAKPWALTAVIVIAGVVGAFAGPRFVPLIVPSKNVKERPFPFTIKLVDGTTIS
                     SRDLGGKIVVLDFWATWCIPCQHELPALQRIYDKTKGQKDVVFVAVDGVITDSPGDVG
                     DSLERATAFFRRGGYSIPLAWDGGAVLEKSFGLTGFPTLVVVDGNGQVRMRRVGYVGA
                     EDLEAILMGQIAELRKEGGR"
     gene            complement(6859..7233)
                     /locus_tag="VNN25_08175"
     CDS             complement(6859..7233)
                     /locus_tag="VNN25_08175"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HXH91542.1"
                     /translation="MPEQTLQEQTAHRWYSRPVLFVDDIGRALRFYMDLLGFEKAWHE
                     GDGKGTVCQVHRAECEIILCEDADRKDRGRLFLSLTKDGIAQLRKEIVERSIPNRKSW
                     WGYDVIQIDDPDGNELLFPISE"
     gene            7278..7577
                     /locus_tag="VNN25_08180"
     CDS             7278..7577
                     /locus_tag="VNN25_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91543.1"
                     /translation="MTQVEDQTEWVSQIASGDGRIFLYKDAAGSQKVSLGGLDIANEE
                     IKDRAVRLTPFDEETNSPRLDAQHGICFMGNFEAEDVLVELLSLDPAGRSQDHIA"
     gene            7798..8235
                     /locus_tag="VNN25_08185"
     CDS             7798..8235
                     /locus_tag="VNN25_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91544.1"
                     /translation="MRWALIALVFGALGCVTTTSVTRTGSGIFAPRGDSAEEEEAPRG
                     LSLSAGSIAADGTVTLELRNYSEEPFVFAGAADRAQFLVEVQAGSTRTRHTVSPWSRQ
                     TVEVPAGERRQLKANIGGASGRVRIGIRSHDLGYVVWTDWIAR"
     gene            complement(8253..8846)
                     /locus_tag="VNN25_08190"
     CDS             complement(8253..8846)
                     /locus_tag="VNN25_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXH91545.1"
                     /translation="MASPDSRVTIHMAASLDGFIARKDGRVDWLETSDEFAGGDTMDP
                     EFVEAFLKTIDCYVMGSRTYETALRFEAEGLGWPYGDKPTFVLTSRDLPRIRDTVELH
                     SGDLAQFVNERLRPAFRSIWFVGGGMVSAECLRLGLADEVRYSILPILIGDGIPFFEK
                     IENDIALHLAEVNAYKNGMVALRYEVRGRGGESQNAT"
     gene            complement(8833..9408)
                     /locus_tag="VNN25_08195"
     CDS             complement(8833..9408)
                     /locus_tag="VNN25_08195"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXH91546.1"
                     /translation="MRCAWARTPLGIAYHDLEWGVPVHDDNILFEFLTLEGAQAGLSW
                     ETILKKRDAYRKAFAGFDPAMVAKFRAAQVARLLANPGIVRNRLKIASTVSNAKAFLA
                     VQRELGSFNAYVWSFVGGTPRMNRWRTPGQVPAKTPQSDALSRDLLRRGFKFVGSTIC
                     YAFMQAVGMVNDHTIDCFRYEHTKASNDGKP"
     gene            complement(9584..>9995)
                     /locus_tag="VNN25_08200"
     CDS             complement(9584..>9995)
                     /locus_tag="VNN25_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH91547.1"
                     /translation="QKRLIAAIERQAKFEALAANAPKEPLVETKSNTDAVYNDPALTQ
                     RMVAAARAAMGAGQVVEMPAQMGGEDFSQFGLAGVHAVLLHVGAVDAAKLEASHKSGV
                     PLPGVHSPLWAPEREPTIKAAMRAETAILMDLMH"
BASE COUNT         1936 a         3073 c         3078 g         1908 t
ORIGIN      
        1 ctcgataccg acacgacgga ctcgatggat tccgagacct ggatactgca ccagaatcat
       61 ttccagatcg aagcgatggc gaatctcgac aaagtcccgc ccgccggcgc cgtgatagtc
      121 gtcacctggc ccaaggtgga aaacggcgtc ggcttcccag ccagagcgtt cgcgatattg
      181 ccgtaggagt gcaggcggga aattcgttca ccacagaggc acaaaggtca cagagatacc
      241 tctgtgctct ctgtgtctct gtggttgcct ttgcttcggc gaccacggat cacctcacgt
      301 ccgcttgcgc aaaatgacat gcgcggcggc acgtccgccg gatacgtgct tcgtgcactc
      361 gtagccgagg gcacgcagat cgatgccgtg gaggagatgt tcgccgcggc ctagcaaaac
      421 cggggagatg acgaggtgca gctcatcgat caggccggcc tgcagatact ggcggatcgt
      481 agagacgcca ccgccgaggc ggacgtcctt accttgcgcc gcgtcgcggg cttgttgcag
      541 cgcttcttcg attccgccgg tgacgaatcg aaattccgtc ccgcccttca tcgccagcgg
      601 cgcgcgcgga tgatgcgtca gcacgaagac gggcacgtga tacggcggtt cctcgcccca
      661 ccaccctttc cactcatcgt ccggccaggg accgcggacc ggcccgaaca tgttccgccc
      721 gagaatccag gcgccgatgc cgtcgaagct ctgcgaggcc atgtcgtcgt cgacgccgct
      781 ctcgcctcct tcgccgccgt gcatttgctg ccaggtgcgc gtgtgaaaaa accagtcgaa
      841 catctccggt ccgttgacgc cgagcgggtt ttcaagatcc tgattcggcc ccgcaccgaa
      901 cccgtcgatc gagatggaga aactgagtac gcgtagtcgt gacatacgtc ggaatggtat
      961 cgcagtggaa gtggggtcag gtccgaagtg tgaagtgacg gaaggggact cctgcctcga
     1021 ggagcggccc tgattccgtc acttcacact tcagacctga ccccaatccg gcatatcgtc
     1081 cacgttatgg tcttgttcga ctcgatcctc gcctggctcg tctccgtcgt cggtgtcgcc
     1141 actggtgccg cctacattcc gcaagcgctg cggatctgga agcgccgaag ctccgacgac
     1201 gtctccatcc tgacctacct cctcttcctc ggcggacaga tcgtctacct cgtctacgga
     1261 atccgcatcc gccagtggcc actgatcatc ggcatggccg cgaatatcgt ggggagcctc
     1321 gccgtgatcg ggagcgcgct aaggttccgg gcggggaggg gtggaccgaa ggagtgagac
     1381 tcggagccgc gttgcaggat gggtagattg cggcgatggt tcgttacacc caattgaatc
     1441 ctgggcttgt tccagaccat tcaagctatc aaggccggat ccaaccaccg aggcgggacg
     1501 ccccgatggc cgcgggcgag acgcccacac tccttgtgcg catcgaaagg agtgcggtcg
     1561 tctcgaccgc gggcagtcgg gcgtcctcgc ccgagtgctg tcagggcgag gtcaaattcg
     1621 tcgacgttct tgcttgcgtt gagcacgatc attgcgctgg cggcaagctc gacgctccca
     1681 ttcgtctgat gcaggaggat ggcaagtccc tcctcgagat caatcgtgac gggtactcaa
     1741 gctccgtccc ttccaaacga cctcctccac cgcgaggccg atcgccagaa tcctgcggtc
     1801 ggtgttttgt gtgcctgcga tcatcaggcc gacgggggcg ctgccggggt cgtggcaggg
     1861 gagggagagg gcgcagccat cgaacaggtt gacgactgat gggttgcgca gcatgcggcc
     1921 gttgaggcgg gagtaggtct cgtcgtcctt gctggcttcg gtgatcgtcg gcgcggtgtc
     1981 gggagttgtg ggcatcagca tggcgtcgta accgctggca gcggcgttga tcgatttgat
     2041 gaagcgctcg cgcaactgaa gcagctcgat gtagtccgcg gcggtgatcg tctcgccggg
     2101 tcgaatgcgg gcgataacgc gagggtcgta ttggtctgcc ttctctttca tccattgccg
     2161 gtgcaatgaa aaggcttccg cgctggtcag cgcggcgcgc gggtttacga cggcagcttg
     2221 tgtgaattcg atcatcggca gctcgatgat gattgcgccg gcagccgaca atcgatccag
     2281 cgctgcggtg aaggcgcggg cgacggccgg tgacaagtcg tcctggaaca ccgtcttcgg
     2341 caccgcgaaa cgaaggccgc gtagatgggt agccgtcgga acactgtcgg tctcggcggc
     2401 cagcacatga tccagcaacg cgcagtccgc gacacacttg gcgatcggcc cgattgaatc
     2461 gagcgtgaac gagagcggca gcacgccttc gcgcggcacg cgtcgcgcgg tcggtttgaa
     2521 accaacgagc ccgttgagcg cggccggaat gcgaaccgat ccgccggtat cggttccgat
     2581 tgcgccggcc gccatgccgt cagtgaccga gatcgcggca cccgatgacg agcctccggg
     2641 aatccggcct gcagcgcgat cgaacacgtt cctcggcgtc ccgtagtgcg ggttgatgcc
     2701 gaggccggag aatgcgaact cggtgaggtt ggtgcggccg atgatcaccg cgccggcgtt
     2761 tcgcaatcgc tcgacgacgg tcgcgtcgcg tgtcgccggc ggcgagccca ccagtacttt
     2821 cgagccgccg agggtgacga cgccggcttc gtcgaacaag tccttgatcg agatcgggat
     2881 gccggccagg acgggcagtc ttgcaccgcc gcgtcgttgc gcgtcaacac ggtcagcggc
     2941 agcaagcgct tcgttctcgt ggacgctgag gaaagtacgc gcgccctctc cctgcgggtc
     3001 tttgatcgcc gcaagcgact gctcgaccag ttgccggctg gtgaccttcc cgctgtccag
     3061 aagccgcgaa agctcaacga tggtcatggt tgcgagctcg cgtcgcggac cgcgctgcgc
     3121 aaacgcgggg gcgacgcgcc aaagcgccgt gccggcaagt gcgagggcag agtcgtacag
     3181 gaagtctcgt cgatcctggt ccatgcatag ttactccttg tcgaacgttc ggtccgtgcc
     3241 ggccaaacga cccgagatca ccgcctcggc agcaggcgac atcgtatcgc tcagcggcca
     3301 ggcaatgaga ctcgagtggc catttctccc aaccatcttc gattcgaagt aaccttcggc
     3361 gccttcgccc gtcttaagag atggaggcat agaaggaaaa tgatcaaaca catcgctgtt
     3421 gccattgctt tgagtctcgc tcccgttgca ttcgcgcaga tgtacatcgt tccggagggc
     3481 gattgcggag cagtcacact tcacgtcaca cgcggcaccg atttcccgaa tctcggcgaa
     3541 gcgatcagcg cggatcgcgt caaggatgcg catgtggttc gggatagacc gatcgagaga
     3601 gtcatcgtca cacccgtcgc cggaccgcgc tcgctcgact tcagcgcgaa cgtccccgac
     3661 cgcgaaggag tcgtcatggc cgctgtcgat ctcaagccgg tggtcagcgg caacgagacg
     3721 cggaccgagc acgccaaagc gttcctcttc tgcggcgcga ccacgccacg tgccgactgg
     3781 caattggagg ctggcctcgg cctggagatc atcccgcaat ggaatgggat caggccgaaa
     3841 atgaagcaag gcgacgaaat gcagttcatc gtcgtgcaca agacgacagg ccacccgatt
     3901 caagacgttc cggtggaact ttacggcgcc ggtgagggcc gaatcggagc cggcgttccc
     3961 aatcagtccg gaggcgtgag ttttccgtac ccaaagccgg gccgctacat ggtcacgacg
     4021 acgtatcggc gtcccgatcc acaacaagcg gagcattggc tcgtcgacac gagcacgctg
     4081 acgttcgaga tcaagtgacg cagcggagct acttctcccg cactactccc aacacattcg
     4141 ggagtggcca ttgcttgtga ttgctgtcgt ccggccagaa tcccgtctcg tagcttccgg
     4201 ccagatcgag atcgatgcct ggtgcatgaa tcgagaggct tgcctcgggg ccgccctcga
     4261 catgcattgc ggccacgatc gcgagcggga gcttcagaag gaggtcgtta aactcgcgca
     4321 tggtgtaggg cgcgcgactg aagagaaaca gcacgcgccc ttgcgaatcg atggcgacgg
     4381 cggcctcgct ccatcgctta tcgctctttg cccagacatt tcgccggcct cgtgcgatga
     4441 gacgcaggtt ttgaacaacg atctcatagc gtgcaagttg ctgcacatcc ttcgtctgat
     4501 cgcgatcgag ccacagtacc cgaggatcgg aagcgcctga cgagttgaga gccaggatcg
     4561 agttgtagtc gttccatcgg ggattgttca cgtgacttcc gtgccgcagg tagcccacat
     4621 tcgactgtcc gtcgttctga aacatgccga ggttgatcgc gacagccagg ttggacgtgc
     4681 gacaccattc ggcggcagtg cgcgcggctg tctttgtctc ggatgcaagc gccaccgtga
     4741 gcttcgcgcg agctggatcg actcgcacga catacagccg gttacccgga tcggatggcc
     4801 ctttgacggc aatcgacgcg agctcagtac ctggttggat gagttgccat gagagatcgt
     4861 ctccctgcgc aggaaccagt gcgctcagga tcaggagcgc ggcgacgacg aaggccgtat
     4921 ggcatcgcct cttcacaggt tgcgagggta ccagacgggc tgaggcgagg cacgattccg
     4981 gaccttccgg gcgcgaaccg gaaaccttcg gaagatcact tgcgtcaaat agtcatgatg
     5041 acgattggtg agaggtttcg aacttccgta gttgccgcct tgatcagtgc tggcgtaact
     5101 tggttatgcg ctcctgcgca cttccaggaa catgagcacc gcgaggacgt cccgcgaatt
     5161 cacgccgcgc ccgaaacgag caccgctccc gttccctcgt tgcgccttcg cgacgcagcc
     5221 gcggacgacg cacgcgtcca cacactacgg cgaaatctat tcgaattcgc ggaggtccag
     5281 gcaaccaagc caaaggtctc gataatctcg caaggatcac ctgcggtcgt cccggttacg
     5341 gtggcagttc tggagacgcc ggtcgttgcc tcaccacctc cgctcgtctt cggatatcgc
     5401 tttctcggaa ccttcggtcc ggcagcgtcg ctgatcgccg cgttcgccgg caacggcgag
     5461 gtcttgaccg ctcgcgaagg agaccacatc ggccagagct tcgtcctccg ccgcatcgga
     5521 acgagggacg tcgaggtggc agcgctcggt cagcaggcga cgctgcgtgt gaatatcgat
     5581 ccgtaagcgt gaatcagaga tcagagataa gcgatgagag attgatgtgt catcctgagt
     5641 cgccgcaccg ccgcaagatt cgctgacctt gcatccttca gacattggcg gcggaggacg
     5701 tcgaaggatc tcaaattacg aagatctcgt tcgagcgtct ttgatcacgt tcgctcgcgc
     5761 tggacgaaca cgatcttcgt aatttgagat ccttcgacgt cctccgccgc cgcgcacgcg
     5821 ctacgcaaac atcgcgattc gcgcggggcg gctccggcag tctcaggatg acaccaaccc
     5881 cggttcgacg atcactcggc cgccactacc gcccgccctc cttccgaagt tctgcgatct
     5941 gtcccatcag aatcgcttca agatcttctg cgccgacata accgacccgg cgcatacgaa
     6001 cttgcccgtt gccatcaacg accaccagcg tcgggaaccc ggtcagcccg aacgactttt
     6061 ccagcaccgc gcctccgtcc cacgccaagg gaatggaata gccgccgcgc cggaaaaagg
     6121 cggtggcacg ttccagcgaa tctccaacgt ctcccggaga atcggtgatc actccgtcga
     6181 cggcgacaaa cacgacatcc ttttgcccct tcgtcttatc gtagatgcgc tgcagcgccg
     6241 gcaattcgtg ttgacaggga atgcaccacg ttgcccagaa atcgagcaca acgatctttc
     6301 cgccgaggtc gcgggaggag atcgtcgtgc cgtctacgag cttgattgtg aatgggaagg
     6361 gcctctcttt gacgttcttg ctcgggacga tcaacggcac gaaacggggg ccggcgaatg
     6421 cgccaacaac accggcaatc acgatgactg ctgtcaacgc ccacggcttt gcgcgcagcc
     6481 gtgccatcgc aatgccggcc gatacggaca cgaacgtgag gactggaaag aggatgaacg
     6541 gcttgttccg gccggagaag aacgcgagcg cgaagagcag caggggaatg ttcgtcagaa
     6601 cagcggtcac ccagggcggc agcgcgtttc tcgacccccg ccagtagccg gcggccactg
     6661 ctaccgtgat cagaaccgga aagcagaggt agacaaggtg aggactctgg agcagcacgc
     6721 ggccgtcgaa aaacgcgacc aggacgaata gcaggccggc cgagagatcg accagcgcgg
     6781 agaggacttt gcgcattttg cgaaccttcc cgcaatgcct acgcagaacc gccggtgtag
     6841 ttgcagcgct cgaagtactc actcggaaat ggggaagagc aactcgttgc cgtcgggatc
     6901 gtcgatctgg atcacgtcat atccccacca cgactttcgg ttgggaatcg agcgctcgac
     6961 gatctctttc cgaagctgcg cgatgccatc cttcgtcagc gacaggaaca gccgccctct
     7021 gtccttgcga tcggcatcct cgcagagaat gatctcgcac tcagcgcgat gaacctgaca
     7081 caccgtgccc ttgccatcgc cttcatgcca cgccttctcg aagccgagca gatccatgta
     7141 gaagcggagc gcacgtccta tgtcatcgac aaagagaacg ggacgcgagt accacctatg
     7201 cgcggtttgc tcttgtaagg tttgctctgg catcgtcgct ccagatcctc cgaatcgcgc
     7261 taccctgcgc ttgcggtttg acccaggttg aagaccagac cgaatgggtc tcgcagatag
     7321 catcggggga cggacgcatc ttcctctaca aggacgcagc cggcagccag aaggtctctc
     7381 ttggcggctt ggacatcgcg aacgaggaaa tcaaagaccg ggccgtgcgg ctcacccctt
     7441 tcgacgaaga gacgaatagc ccccgtctcg acgcgcagca tggcatctgt ttcatgggta
     7501 acttcgaagc cgaggacgtt ctcgtagaac tcctgagcct ggacccagcg gggcgttctc
     7561 aagatcacat cgcgtgagga ctggagaggg ttggtcatcg tgcctgctac cggcaatagg
     7621 cctttgtggc tggcctgcgc cgcctgaggt tccaagcgca gcagcctgga gccgcaagcg
     7681 aaaacgtatc acgcatcgga ggaggggagg gaggaacgtc cgatgaccga tgagcgaagg
     7741 cgtgaccctc cgggacttcg ggtgacgctg tagacttgcg cgaagggaat gccgaccatg
     7801 cgatgggctt taattgcgct cgtcttcggg gcgctcggat gcgttacaac gacgtccgtc
     7861 acgcgcacgg gctcaggaat cttcgctccg cgcggagact ccgcggaaga ggaggaggct
     7921 ccgcgtggac tgagtctttc cgccggatcg atcgccgctg atggaacggt caccctcgag
     7981 ctgcgcaact attcggagga accgttcgtc tttgcaggcg cggcggatcg cgcccagttc
     8041 cttgtcgaag tacaggcggg tagtacgcgc acgcggcaca ccgtttcgcc gtggagcaga
     8101 cagacggtgg aagtgccggc gggggagcgt cgccagttga aggcaaacat cggcggagca
     8161 tccggccgcg tgcgcatcgg catccgcagc cacgatcttg gatacgtcgt ctggacggat
     8221 tggatcgcgc gatagacggc acgccggttg agtcacgtgg cgttctgtga ctcgccgccg
     8281 cgtccccgca cctcgtaacg aagcgccacc atgccgttct tgtaggcatt gacctctgca
     8341 agatggaggg cgatgtcgtt ttcgattttc tcgaagaacg ggatcccgtc gccaatcaag
     8401 atcggcagga tcgaatagcg aacttcgtcg gccagcccga ggcggaggca ttcggcggag
     8461 accataccgc ctccaacaaa ccagatggaa cggaacgcag gccgcagacg ctcattcacg
     8521 aactgcgcga gatcgcctga gtggagctcg acggtatcgc ggatccgagg aaggtcgcga
     8581 ctcgtgagga cgaaggtggg tttgtcgccg tacggccagc ctaacccctc cgcttcaaaa
     8641 cgaagcgcgg tctcatacgt tcgcgatccc atcacgtagc agtcgatcgt cttgaggaac
     8701 gcctcgacga attccgggtc catggtgtcg ccgcctgcga attcgtccga ggtttcgagc
     8761 cagtcgacgc gcccgtcctt gcgggcgatg aagccatcga ggctcgccgc catgtggatc
     8821 gttacgcgag aatcagggct tgccatcgtt tgatgccttc gtgtgttcat atcggaaaca
     8881 atcaatcgtg tgatcgttca ccatgcccac tgcctgcatg aacgcatagc agatcgtcga
     8941 tcccacaaac ttgaatccgc ggcgcaacag gtcccggcta agggcatcgg attgcggcgt
     9001 ctttgcaggg acttgcccgg gcgttctcca gcggttcatg cgaggcgttc cgccaacgaa
     9061 gctccacaca taggcattga aactcccgag ctcgcgctgg actgcaagga aggcctttgc
     9121 attgctcacc gtactggcga tcttgagacg attgcggaca atgccgggat tcgcgagtaa
     9181 acgcgcgacc tgtgctgctc ggaacttggc caccatcgcc ggatcaaacc cggcgaatgc
     9241 cttgcgatag gcgtcgcgtt tcttgagaat cgtctcccag ctcagcccgg cctgcgcccc
     9301 ttcgagcgtt agaaactcga agaggatgtt gtcgtcgtga accggtacgc cccattcgag
     9361 atcgtgatag gcaatgccga gcggcgtgcg agcccaggca catcgcacgg attgatccgc
     9421 agtcttactt ttgtttgaat ggtgatcact tttgattctt tcgctgacca tgcgcagtgt
     9481 cttccgttcg ttttgcgggg gtcgcatgtc gggggtcggt tgtcggagtt gatatggttt
     9541 tgctcccacc ccccactccc gactccccac tccctcagaa atcctaatgc atcaaatcca
     9601 tcaggatcgc cgtctccgca cgcatcgctg ccttgatcgt cggctcgcgc tccggcgccc
     9661 ataacggcga gtgcacgccc gggagcggca cgccggactt gtgcgacgct tccagcttcg
     9721 cggcatcgac tgcgccgacg tgcagcaaca cagcgtgaac gcctgccagt ccgaattgcg
     9781 agaagtcttc gccacccatc tgtgcaggca tctcgaccac ctggccggcg cccatcgcgg
     9841 cgcgtgcggc ggcgaccatc cgttgcgtca gcgcggggtc gttgtagacg gcgtcggtgt
     9901 tggacttggt ctcgaccagc ggttccttcg gcgcattcgc ggcgagcgct tcgaatttcg
     9961 cctggcgctc gattgcggcg atcaggcgct tctgg
//
LOCUS       DATLVW010000258         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_462952, whole genome shotgun sequence.
ACCESSION   DATLVW010000258 DATLVW010000000
VERSION     DATLVW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462952"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VNN25_08205"
     CDS             complement(<1..718)
                     /locus_tag="VNN25_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91548.1"
                     /translation="MTSVLQQRADRAYGFPFRGCVSSVDRHEVPLWACGPDSRELLAE
                     PGREDDPIPTGKLKRRHGAAGVGSNAGILELLAADTTFKSKRLLSLSTPATADGFDTI
                     GSIWGDEAKRFGQVAIHDGATDVIEGEFETSNFHYFNGARMDIIAPPLVYNGNGATGY
                     HRYSNSFLRGKTISGSRNSVGIKNREYFGGFDSAATNWNRSYNTATGSGTAQVVRFPT
                     GLVMPLRAATRVDGDYPAIGG"
     gene            complement(715..2475)
                     /locus_tag="VNN25_08210"
     CDS             complement(715..2475)
                     /locus_tag="VNN25_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH91549.1"
                     /translation="MKRLALALVFVASIARAQGWGWTPITAPIPIPPSTIAVRIGTAL
                     QPIVDALPAAGGVIQLDPGRFYADASDSTIALTLSKPVTIIGTTYDATTMASPILISS
                     PDCHLERFRVQPSGAAYGIKIFQSGAGVARSSLRDMFVGAVDTTARNAGQGPVIGLYL
                     DGAILTTIDHCTFAFCTQSGVYLNSSGLPNWSTNENIFTNCTSNLSSHYGVEIIGGGI
                     QGFQWLGGNMEDNTWGAVFVQSATNLTFRGVDFESTVFPIADLLSIATSAPSTIEDCN
                     FNTSGVTRAFNFFTCAQVSVLRNRLTTFSAYSNAGTFDDRSSNCIEWGNVFNTGGYIE
                     NRTQPGIPSLAGLFQPNLRSYYGVLWGPSAAGTAATIGSAWTATGAVTNPAPTTSHLS
                     GWLRRTVFTGAAAAETGIKQTGASDFQFWSGDSAGTGGFYFSATFRLDAWPANTGRLF
                     VGLVASGSVAPASVADSTGLTGDAIGLYHDANDVVNVFRIVAVKAGVATKFALTNGAT
                     APVLDNSQGFRFEMIAWPNANAVYCRLYSLNKYGTLAGLVTLTNAITKTALMAPQVEV
                     GSAAGTYSIGVANVTCQVAR"
     gene            complement(2472..>2839)
                     /locus_tag="VNN25_08215"
     CDS             complement(2472..>2839)
                     /locus_tag="VNN25_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91550.1"
                     /translation="DRQIFGSAWTAWRQGATPSSGNVGKFNFAVWPVSSVVHDYLLGV
                     LREVTALVNQTDIPDGSEEDHHYVCDMTSLITGRWMGRSEEWLGDVRARLPEQLLTAT
                     HQLAKDIGLVKPRPGQEAA"
BASE COUNT          497 a          880 c          944 g          518 t
ORIGIN      
        1 gtccaccgat cgccgggtag tcgccgtcaa cgcgggtcgc ggcacgcagc ggcatcacga
       61 gcccagtcgg gaagcggacg acctgggctg tgccagagcc ggtagccgtg ttgtacgacc
      121 ggttccagtt cgtggccgcg ctgtcgaatc cgccgaagta ttcgcggttc ttgatgccga
      181 cgctgttgcg cgatccggag atggtcttcc cgcgcaggaa cgagttggag tagcggtgat
      241 acccggtcgc accgttgccg ttgtagacga gcggcggcgc gatgatgtcc atgcgagcgc
      301 cgttgaagta gtggaagttg ctcgtctcga actctccctc gatgacgtcc gtggcaccgt
      361 catgaatcgc gacctgcccg aacctcttcg cctcgtctcc ccagatgctg ccgatggtgt
      421 cgaagccgtc ggcggtcgcg ggcgtgctga ggctcaggag ccgcttgctc ttgaatgtcg
      481 tgtcggcggc gagcaactcg aggatgccgg cgttggagcc taccccggcg gcaccgtggc
      541 ggcgtttcag cttgccggta ggaatcgggt catcctctcg tcccggttcg gcaagcagtt
      601 cgcgcgaatc cggcccgcag gcccagagcg gcacctcgtg cctgtccacg gacgacacgc
      661 agccgcggaa cgggaaaccg taggcgcggt ccgcgcgctg ttggaggacg ctcgtcatcg
      721 cgccacctga caggtcacgt tggcgacgcc aatggagtaa gtgccggcgg cactgccgac
      781 ttcgacctgc ggagccatca gtgcggtctt agtgattgcg ttcgtcagcg tcaccagacc
      841 ggcgagcgtt ccgtacttat tcaagctgta gaggcggcag tagacggcgt tggcattcgg
      901 ccacgcgatc atctcaaagc ggaatccctg ggagttgtcg agcaccggcg cggtcgcgcc
      961 attcgtcaat gcgaacttgg tcgccacgcc cgccttgacc gcaacgattc gaaagacatt
     1021 aacgacgtca ttagcgtcgt gatataggcc gatggcgtcc cccgtgagtc cggttgagtc
     1081 cgcgacggaa gccggagcta cggacccgct ggcgaccagc ccgacgaata gccggccggt
     1141 attcgccggc catgcatcaa gccggaacgt ggcggagaag tagaaccctc cagtcccggc
     1201 cgaatcgccg gaccagaact ggaagtcgga ggcacccgtc tgcttgatgc ctgtctccgc
     1261 cgccgcggct cccgtaaaca ccgtgcggcg aagccagccc gacagatgcg acgtcgtcgg
     1321 agccggattt gtgactgccc ctgtcgccgt ccacgcgctt ccgatcgtcg ccgccgttcc
     1381 tgcggcggat gggccccaca ggactccgta gtacgagcgc agattcggct gaaagagtcc
     1441 ggccaggcta gggatgccag gctgcgttct gttctcaatg tagccgccgg tgttgaaaac
     1501 gttcccccac tcgatgcagt tggaagaccg gtcatcgaac gtgccagcgt tggagtaagc
     1561 gctgaacgtg gttagacggt ttcgcagcac gctaacctgg gcgcaggtga agaagttgaa
     1621 tgcgcgcgtt actccactag tgttgaagtt gcaatcctcg atcgttgacg gggcggacgt
     1681 cgcgatgctg agaaggtctg caatcgggaa gactgtggac tcgaagtcca cgccgcggaa
     1741 cgtaagattc gtcgctgatt gcacgaatac cgcgccccat gtgttgtcct ccatgttgcc
     1801 gccaagccat tgaaagccct gaatgcctcc gccgatgatc tcgacgccgt agtgcgaact
     1861 gaggttcgac gtgcagttag tgaagatgtt ctcgttcgtg ctccagttcg gcagcccgga
     1921 ggagttgagg tagacgccgg attgcgtgca gaacgcgaac gtgcagtggt cgatggtcgt
     1981 caggatagcg ccgtcgagat agaggccaat gacaggcccc tgaccagcgt ttcgagccgt
     2041 cgtgtccaca gctccgacga acatgtctcg tagggaagag cgagcgacgc cggccccgga
     2101 ttggaagatt ttaatgccgt aggccgcccc ggacggctga accctgaacc gctcgaggtg
     2161 gcagtccggg ctgctgatga ggatcggcga cgccatcgtc gtggcgtcgt acgtcgtgcc
     2221 gatgatggtc acgggcttcg acagcgtgag cgcgatggtc gagtcggagg cgtcggcgta
     2281 gaagcggccg gggtccagtt ggatgactcc gcctgccgcc gggagcgcgt ccacgatggg
     2341 ctgaagcgcg gttccgatcc ggaccgcgat ggtggacggc ggaatcggga tcggcgccgt
     2401 gatgggcgtc caaccccaac cttgcgcccg ggcgatggag gcgacgaaca ccaatgccaa
     2461 ggcgagtctt ttcacgccgc ctcctgcccg ggccgcggct tcacgagccc gatgtccttc
     2521 gccaactggt gcgtcgccgt gagcaactgt tccggcaggc gcgcgcggac gtcgccgagc
     2581 cattcctccg agcgtcccat ccaccgcccc gtgatgagcg aggtcatgtc gcagacgtag
     2641 tggtggtcct cttcggagcc atcggggatg tccgtctgat tgacgagcgc cgtgacctcg
     2701 cgcagcacgc cgagcaggta gtcgtggacg acgctcgaca ccggccacac cgcgaagttg
     2761 aacttgccga cgttcccgct cgacggcgtc gcgccctgcc gccatgctgt ccacgccgag
     2821 ccgaagatct ggcggtcgg
//
LOCUS       DATLVW010000259         5809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_10260, whole genome shotgun sequence.
ACCESSION   DATLVW010000259 DATLVW010000000
VERSION     DATLVW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10260"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..988)
                     /locus_tag="VNN25_08220"
     CDS             complement(59..988)
                     /locus_tag="VNN25_08220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH91551.1"
                     /translation="MSAGVKVSVIMPVERIGGDAERAIESVLRQEAPFAFELILVSAE
                     PLTMQPGNAVRNVIEPDRNPATRRNRAVSESQGEILAFIDDDATADTGWLATAVSYLD
                     AHPDIVVLGGPDPAPADSTRAELIAETLLATPLIGSGLACHENRPGIFPVTSASDIAL
                     VNLFVRRSAFRGFDESIGYIGEDTSLIATLMPAVVYHSGVRVFHRRRPFPGPYLRQRW
                     RYRVKTGEMLRRGSAAYAKNRKIWAFLIVGTIAILASPVAIVPYFVFTLILSARVTRL
                     PRRDWIVLPFAFAAHHAIYFAGIVWGAIRGRVR"
     gene            1069..2301
                     /locus_tag="VNN25_08225"
     CDS             1069..2301
                     /locus_tag="VNN25_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH91552.1"
                     /translation="MKLIVQIPAFNEEATIAQTLRDIPKKIDGITAIETLVIDDGSSD
                     NTAAAARKAGATHVVQLKSHRGLASAFLAGIDASLRLGADIIVNTDADNQYAGSDIPK
                     LISPIMRGTADVVIGDREVAKSPHMSAIKRLLQRIGSRTVGLASGLRVGDVTSGFRAF
                     SREAATQINVFNPFTYTLETIIQAGNRNLGVQSVPVRTNAPTRPSRLYHGIGTYLRKS
                     AATIFRIYTIYKPLKTFFAIGTVLLLAGTILGGRFLWHFFQGDRAGHIQSLILAAVFL
                     IIGFQTLLIGLVADLISVNRRLSEEVLVRLKRAEAPPAVERRPQPAIRDVRPARPQPQ
                     PRRERPAAPPEAKTASTQWVWLLDEDKLHDRVKPAETPILEPDLDDDGPDEQPVAVAG
                     GPRRRRRRRGPRPKPAGE"
     gene            complement(2400..3194)
                     /locus_tag="VNN25_08230"
     CDS             complement(2400..3194)
                     /locus_tag="VNN25_08230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH91553.1"
                     /translation="MPFTILSDEGLPIRGDLDVPAGAKALVVIVHGFKGFKDWGFFPW
                     LGEMLCNDKFAVCRFNMSRSGIGEQPDIFDRLDLFAEDTYSGQIADLQKVVRFCQERV
                     DAPTFLVGHSRGGGVALLAADGIANLSGIVTWSSIARTDRYDDATKSMWRRVGYVEIE
                     NSRTKQIMRMSTRMLDDIEANRDRLDILKCTTQIEQPILVIHGARDESVPAAESLEIA
                     SYARDASRVIVAKASHSYNAIHPLIHVPFELSMAAAVTSRFVSVYA"
     gene            complement(3212..4306)
                     /gene="ychF"
                     /locus_tag="VNN25_08235"
     CDS             complement(3212..4306)
                     /gene="ychF"
                     /locus_tag="VNN25_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXH91554.1"
                     /translation="MGLSVGIVGLPNVGKSTIFNALTAAGAQSANYPFCTIEPNVGIV
                     PVVDPRLDKLAELAQSKQTVYTDLRVVDIAGLVRGASKGEGLGNQFLANIRETDAILH
                     VVRCFDDPNVVHVEGSVDPERDIEVIETELLLADLDAVSKKLDRVARLARVGQKEFVA
                     QAEALEKAKAAIDAGTPLRAVDFSDEAAESLRELNLITLKPVLYVANVTESDLGKDTP
                     MLQKVRSVAAQKKGEIVVICGELEAQISQLPPAERADFLNEMGLQESGLSALIRAAYK
                     LLDLETFFTAGPKEARAWTINNGSKAPQAAGKIHSDFERGFIRAETIAYNDYVSLGGE
                     PGAKAAGKMRSEGKDYVVQDGDVILFRFNV"
     gene            complement(4806..5759)
                     /gene="glk"
                     /locus_tag="VNN25_08240"
     CDS             complement(4806..5759)
                     /gene="glk"
                     /locus_tag="VNN25_08240"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HXH91555.1"
                     /translation="MKIVAGDIGGTKVLLQLVDVSQAGRLVSAEERYESNAYATFDAM
                     LKAFFDAHVAGPVAAACFAVAGPVFGDHAEVTNLTWKMDATALAKAFSIGRVSLINDF
                     YAIALGVPILAHGDFLVLNAGSRVNFGPIAILGAGTGLGQANLVHEGAKWNVVPSEGG
                     HADFAPQDEEQTRLFLSLHAKYGHVSWERLLSGAGLVNIHNFLTGEERPYDETLPMEI
                     AKALAAGDATAARTFAIFVDIYGAEAGNMALRLLARGGVYLAGGVAAKNVEQFTDGRF
                     MQAFLRKGRFQHILADIPVNLITNPKVGLLGAAEMAARMVA"
BASE COUNT         1108 a         1841 c         1813 g         1047 t
ORIGIN      
        1 tggcgaagcc ggatgagggg gcgcttgact gtcgcgcacc attagaacag gggctgcact
       61 aacgcaccct cccgcgaatc gccccccaga cgatgccggc gaagtagatg gcgtggtgtg
      121 cggcgaaggc gaacggcagc acgatccagt cgcgcctcgg caggcgggtg acgcgcgcgc
      181 tgaggatgag cgtgaagacg aagtacggaa caatcgcgac aggagacgcc aggatcgcga
      241 tcgtgccgac gatgaggaag gcccagatct tccggttctt cgcatacgcg gccgaaccgc
      301 ggcgcaacat ctcccctgtc ttcacccgat agcgccaacg ctggcgaagg tacggaccgg
      361 gaaacggacg gcgtctgtgg aacacgcgga cgcccgagtg atacacgacc gcaggcatga
      421 gcgtagcgat cagcgaggtg tcctcgccga tgtagccgat cgactcatcg aatccgcgaa
      481 aggcactgcg gcgaacgaac agattcacca gcgcgatgtc gctcgcgctc gtcaccggaa
      541 agatgcctgg ccggttctcg tggcaggcca gaccactgcc gatgagcggt gtcgccagca
      601 gcgtttccgc gatgagctcg gcgcgcgtcg agtcggctgg agcaggatcg ggaccgccca
      661 gaacgacgat gtcgggatga gcatcgagat agctaacggc ggtcgcgagc cagccagtgt
      721 cagccgtggc gtcgtcgtcg atgaaggcca ggatctcccc ctgcgactcg gaaactgctc
      781 gatttcttcg agttgcggga ttacgatccg gctcgatgac gtttcgtacc gcgttcccag
      841 gctgcatcgt caatggctcc gcggagacca gaatcagctc aaacgcgaac ggcgcctcct
      901 gacgcagcac cgactcgatc gcgcgttccg cgtcgccgcc gattcgttcc acaggcatga
      961 tgacggaaac tttgacgccg gcggacatgg gtggtgcgta gattagtgga ttcaccactg
     1021 atccactgat ccacgaatcg actaatctac ggattcacta cacactgcat gaagcttatc
     1081 gttcaaatac cagccttcaa cgaagaagcg acgatcgccc agacgcttcg cgacatcccg
     1141 aagaaaattg acggcatcac cgccatcgag acgctcgtca tcgacgacgg gtcgtccgac
     1201 aataccgccg ccgcggcgcg aaaagcgggc gcgacgcatg tcgttcagct caagagccat
     1261 cgcggcctgg cctcggcgtt cctggccggc atcgacgcct ccctccgcct cggcgccgac
     1321 atcatcgtca acaccgacgc cgacaatcag tacgcaggca gcgacatccc gaagctgatc
     1381 tcgcccatca tgcgcggcac cgcagacgtg gtgatcggcg accgcgaggt ggccaagtca
     1441 ccgcacatgt cggcgatcaa gcggctgttg cagcgcatcg gcagccggac tgttggttta
     1501 gcctcgggac tgcgcgtcgg cgacgtcacc agcggcttcc gggcgttctc cagagaagca
     1561 gcgacgcaga tcaacgtctt caacccgttc acctacacgc tcgaaaccat cattcaagcc
     1621 ggcaaccgca acctcggcgt ccaatcagta ccggtgcgca ccaacgcgcc gacccgccca
     1681 tcgaggctct atcacggcat cggcacctac cttcgcaagt cggcggcaac gatcttccgc
     1741 atctacacga tctataagcc gctcaagaca ttcttcgcaa tcggcaccgt ccttctgctc
     1801 gccggcacaa tcctcggcgg acgcttcctc tggcacttct ttcagggcga tcgcgcagga
     1861 cacattcaat cgctcatcct ggccgcggtc tttctcatca tcgggttcca gacgctgctg
     1921 atcggactcg tcgccgatct catctccgtc aatcgtcgtc tcagcgaaga agtactcgta
     1981 cgcctcaaac gcgccgaggc gccccccgcc gtcgagcgcc gcccgcagcc agccattcgc
     2041 gacgttcgtc ccgcgcgccc tcaaccgcag ccgcgacgcg agcgtccggc agcgccgccc
     2101 gaggcgaaga cagcatcgac gcaatgggtg tggctgctgg acgaagacaa gctgcacgac
     2161 cgcgtgaagc ctgcggagac acccatcctc gaaccagatc tcgatgacga tggtccggat
     2221 gaacagcctg tcgctgttgc tggtggtccg aggcggaggc gtcgtcgtcg tggaccgcgg
     2281 ccgaagccgg ctggcgagta ggttcaaaaa aacatcacca cagagacaca gaggacacag
     2341 aggacacaga gaaaacctct gtgctctctg tgtctctgtg gttgatttgt ctttctgccc
     2401 tacgcgtaca cgctaacgaa ccgcgaggtt actgccgcgg ccatgctcag ctcgaagggg
     2461 acgtggatca gcgggtggat ggcgttgtag ctgtggcttg ctttagcgac gatgacgcgt
     2521 gaggcgtcgc gcgcgtacga ggcgatctcg agtgattcgg cggccggtac gctttcgtcg
     2581 cgggcgccgt ggatgacgag aatcggctgc tcgatctgcg tcgtgcactt gaggatatcc
     2641 agccggtcgc gattcgcttc gatgtcgtcc agcatccgcg tcgacatccg catgatctgc
     2701 ttcgtgcggc tgttctcgat ctcgacgtat ccgacgcggc gccacatcga cttcgtggcg
     2761 tcgtcgtatc ggtcggtgcg cgcgatcgag ctccacgtca cgatgccgga gaggttcgcg
     2821 atcccatcag cggccagaag cgccacgccg ccgccgcgcg aatggccgac caggaacgtc
     2881 ggcgcgtcga cgcgctcctg acagaagcgc acgaccttct gcaggtcggc gatctggccg
     2941 gagtacgtgt cttccgcgaa gagatcgagg cggtcgaaga tgtcgggctg ctcgccgatc
     3001 ccgcttcgcg acatgttgaa acggcagacg gcgaacttgt cgttgcagag catctcgccc
     3061 agccacggaa agaagcccca gtccttgaac cccttgaagc cgtgcacgat gacgacgagc
     3121 gctttcgccc cggccggcac gtcgagatcg ccgcggatcg gaaggccttc gtcagagagg
     3181 atggtgaatg gcatcgttag tagttcgcga cctacacatt gaatcgaaac agaatcacgt
     3241 ctccgtcctg gacaacgtaa tcctttccct cgctgcgcat cttcccggct gctttcgctc
     3301 cgggctcgcc gccgagggac acgtaatcgt tgtaggcgat ggtttcggcg cggatgaagc
     3361 cgcgctcgaa gtcgctgtga atcttgccgg ccgcctgcgg ggctttcgaa ccgttgttga
     3421 tcgtccaggc acgcgcttct ttcgggccgg cggtgaagaa cgtctcgaga tcgaggagct
     3481 tgtaggcggc gcgaatgagc gcgctcaggc ctgactcctg gaggcccatc tcgttgagga
     3541 aatcggcgcg ctcggcgggc ggcaactggg agatctgcgc ttcgagctcg ccgcagatga
     3601 cgacgatctc tcccttcttc tgcgcggcca cgctgcgcac cttctgcagc atcggcgtgt
     3661 ccttgccgag gtcggattcc gtgacgttgg cgacgtagag aaccggcttg agcgtgatca
     3721 ggttcagctc gcgcaacgat tcggcagcct cgtcgctgaa gtcgaccgcg cgcaacggcg
     3781 tcccggcatc gattgccgcc ttcgcctttt ccagcgcctc ggcctgcgcg acgaactctt
     3841 tctgcccgac gcgcgcgaga cgcgcgacgc ggtcgagttt cttactgacc gcgtcgagat
     3901 cggcgagcag cagctccgtc tcgatcacct cgatgtcgcg ctccggatcg acagaacctt
     3961 cgacgtgcac gacgttcgga tcatcgaagc agcggacgac gtgaaggatg gcgtcggtct
     4021 cccggatgtt ggcgaggaac tgattgccga gcccctcccc tttcgacgca ccgcgcacga
     4081 ggccggcgat gtcgacgacg cgaaggtcgg tgtagacggt ctgcttgctc tgcgcgagct
     4141 cggcaagctt gtcgagtcgc ggatcgacga cgggcacgat gccgacgttc ggctcgatcg
     4201 tgcagaaggg atagttcgcg ctctgtgctc cggcggcggt gagcgcgttg aagatcgtcg
     4261 acttaccgac gttcggcagc ccgacgattc cgactgaaag acccatgtgt gtgttgactc
     4321 ctctgatgca acctgccgcg gcgtgaacgg atggggggtg ggtgcgattc ctaaggggta
     4381 gagatcagag acgagagatt acgagagatc agagatcagg aaggcagaag ccgcatcctt
     4441 atctctgatc tctgatatcg ccgatctctg gagtcagggc ttcttgagac gacgcgcgca
     4501 aaacgtgcgg tgtcatcctg agcctgccgg aagcgccagc gcaaaacgtt gctctcgcgc
     4561 agatcatcgg cggcgccgca ggacggcgaa ggatctcaaa atgcgaagat ctcgttcgag
     4621 cgccctgacc acgttcgctc gcgctggacg aacacgatct gcgaagtttg agatccttcg
     4681 acgtcctgcg ccgccgagca tgtactgcgc aaacatcgcg attcgcgcgg gcggctccgg
     4741 cagtctcagg atgacaccaa tcttgattgg actatcactc gggatgacac caatccccgc
     4801 accagtcacg ccaccattct cgccgccatc tccgcggcgc cgaggaggcc gaccttcggg
     4861 ttcgtgatga ggttcaccgg gatgtcggcc aggatgtgct ggaagcgtcc cttgcgcaga
     4921 aacgcctgca tgaagcgtcc gtcggtgaat tgctcgacgt tcttggcagc gactcctccg
     4981 gcgagataca cgcctcctcg ggccagcagc cgcagcgcca tgttccccgc ctcggcgccg
     5041 tagatgtcga cgaagattgc gaacgtgcga gcggcggtgg catcgccggc tgcgagcgcc
     5101 ttggcgatct ccatcggcaa cgtctcgtcg tacggccgct cttcgccggt cagaaagttg
     5161 tggatgttga cgagtcccgc tccggagagc agccgctccc agctcacgtg gccgtacttc
     5221 gcgtggagcg aaaggaagag gcgcgtctgc tcttcatcct gcggcgcgaa gtcggcgtgg
     5281 cctccctcgg aaggaacgac gttccatttg gctccttcgt gaacgagatt ggcttggccg
     5341 aggccggtgc cggcgccgag aatggcgatg ggaccgaagt tgacacgcga tcccgcgttg
     5401 aggacgagaa agtcgccgtg cgcgagaatc ggaacgccga gggcgatggc gtagaagtcg
     5461 ttgatgaggg agacgcggcc gatggagaac gccttcgcca gcgccgtcgc gtccatcttc
     5521 cacgtcaggt tcgtcacctc ggcgtgatca ccaaacaccg gccccgcaac ggcgaagcaa
     5581 gccgccgcga cgggaccagc gacatgcgca tcaaagaacg ccttgagcat cgcatcgaac
     5641 gtcgcgtacg cgttgctctc gtaccgctcc tccgccgaca ccagccgccc cgcctgcgat
     5701 acatcgacga gctgcagcag gaccttcgtg cccccaatgt caccggctac gattttcatg
     5761 gggacattct atggggggtg gggtgttggg ggtggggtgt gggaaaaaa
//
LOCUS       DATLVW010000260         3874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_178682, whole genome shotgun sequence.
ACCESSION   DATLVW010000260 DATLVW010000000
VERSION     DATLVW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178682"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="VNN25_08245"
     CDS             <1..1827
                     /locus_tag="VNN25_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HXH91556.1"
                     /translation="DSPATLAGQYGVGTAAFGTQAFNLSGNVVAASPADGCAAIGASV
                     AGKIAFVNRGTCGFTVKAQNAQNAGALAVIVGNVADSPSASSITKMACSAATCTPSEI
                     ALIPSLLLPVESADAVRTALAAGAIHATLRRDAGIDRDGTVDYSVVAHEWGHYLSNRL
                     IANSAGLISNQSRGMGEGWSDFVALLAMVRPEDTSITSNATFNGAYPMGAFVTSGEAN
                     GPIPNGGYYFGIRRVPYSTEMTRNPLTLRHISNGRPISGTLAFGNDGSTNAEVHATGE
                     VWATMLWECYAALLRDTLGAQPRLTFAEAQQRMKDYLVASLKITPADPTFLDARNALL
                     AAAFASDRTDYLRFWQAFAKRGAGTRAAITERYSTSNAGVTEDFTLGGDAAITVITVD
                     DAAGSCKRNGILEGGEDGVLAVTIRNTGNTHLGATTGHVTTDDPRLNLHGGAITFPAS
                     DPGQSVTGSLSVSLAPGTEALTPIINVTVSDPDFALAESALFTSQIRLNVHEEAKQSA
                     TDDFETPRTAWDIPTSGRWSVLDLSSTQRVWQGTNHFPQTDASLTSPLLTVARELPLR
                     IVFHHRHWFNMTTAANGDLVPLEGGVVELSADDGITWSDIGA"
     gene            2038..2421
                     /locus_tag="VNN25_08250"
     CDS             2038..2421
                     /locus_tag="VNN25_08250"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HXH91557.1"
                     /translation="MPLGWQIDDVAINGITNLPFDTFVADRGLCSTSSSTTEIHALGA
                     LLQATVSSTLATPNGAVEFFENGKTVGAAPLVNGVAAWNPATFLAPGTHTITASFGGT
                     TNFTASTSAPATITIAAPTRRRAAR"
     gene            complement(2711..3172)
                     /locus_tag="VNN25_08255"
     CDS             complement(2711..3172)
                     /locus_tag="VNN25_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91558.1"
                     /translation="MEPHLELQSIHTKVSENDIYDDLLKRLHRDRLHLGEANELVIAK
                     RGTKTRTAAIATAVERARLNFARKWGDRQFPPTKILSSEPSRSAGLQVIDYFLWALQR
                     MFERKEDRYFAPHASKYRLIMDIDDQRRNAYGEWYLEGNPITLEKLVLESG"
     gene            3595..3762
                     /locus_tag="VNN25_08260"
     CDS             3595..3762
                     /locus_tag="VNN25_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91559.1"
                     /translation="MAKKEAVRVTKLTLQKKETANVKAGMTVRGNICCTCSMSGVGKS
                     GKQFGTTTSAA"
BASE COUNT          796 a         1212 c         1145 g          721 t
ORIGIN      
        1 gattccccgg ctacactggc cggccaatat ggggttggta ccgcagcctt cggcacacag
       61 gccttcaatc tgagcgggaa tgttgtcgcg gcatcgccgg cggatggctg cgcggcgatc
      121 ggcgcctcgg tcgccggcaa gatcgccttc gtcaatcgcg gcacctgcgg cttcacagta
      181 aaggcacaaa acgcacaaaa cgcgggcgcg ttggcggtga tcgtcggcaa cgtcgccgac
      241 tcgcccagcg catcgagcat cacgaaaatg gcttgcagcg ccgcaacttg cacgccatcg
      301 gagattgcgc tgatcccgtc cctgctgctt ccggtggaaa gcgcggacgc ggttcgcacc
      361 gctctggccg ccggcgccat tcacgccacg ttgcggcgcg acgcaggcat cgatcgcgac
      421 ggcacggtcg actactcggt cgttgcgcac gaatggggtc actacctgag caatcggctc
      481 atcgccaatt cggcaggttt gataagcaac cagtcgcgcg gcatgggcga gggatggagc
      541 gacttcgtgg cgctgctggc gatggtgcgc cccgaggaca cgtcgattac gtcgaacgcc
      601 acgttcaatg gtgcatatcc catgggcgca tttgtcacga gcggcgaagc gaacgggccg
      661 attccgaatg ggggctacta cttcgggatc cggcgcgtgc cctattcgac cgagatgacg
      721 cgcaatccgc tgacgttgcg gcacatcagc aacggcaggc ctatcagcgg cacattggct
      781 ttcggcaacg atggcagcac caacgccgag gtgcacgcca ccggcgaagt gtgggccacg
      841 atgctgtggg aatgctacgc ggcacttctt cgcgacacgc tcggggcgca accgcgtctg
      901 accttcgccg aggcgcagca acgcatgaag gactatctgg ttgcgtcgct gaagatcaca
      961 ccggccgatc cgaccttcct cgatgcccgt aatgctctgc tggccgctgc gttcgcatcg
     1021 gatcgcaccg actatctgag gttctggcag gctttcgcaa aacgaggcgc gggaacgcgt
     1081 gcggcaatca ctgagcgcta ctcgacgtcc aacgccggcg tgacggaaga cttcacgctc
     1141 ggcggggatg ccgccatcac cgtgatcacg gtcgatgacg cggcaggcag ttgcaaacga
     1201 aatggcattc tcgaaggggg cgaagacggt gttcttgccg tcaccattcg caacaccggc
     1261 aacacgcacc tcggggcgac gacgggtcat gtgacgacgg acgatccgcg cctgaacttg
     1321 cacggcggcg cgatcacgtt ccctgccagc gatcctggcc agagcgtcac cgggagcctg
     1381 agcgtctccc ttgctcccgg caccgaggcg cttacgccga tcatcaatgt cacggtcagc
     1441 gatccggact tcgctctggc cgaaagcgct ctgttcacgt cccaaatccg gctcaacgtg
     1501 catgaagagg caaaacaatc ggcaacggac gatttcgaga ctccgcgaac cgcgtgggac
     1561 attccgacaa gcggccgatg gagcgttctc gatctctcgt ccacgcagcg cgtctggcaa
     1621 ggtacgaatc atttcccgca gaccgacgcg tctctgacct cgccgctgtt gaccgttgct
     1681 cgcgagcttc cgttgcggat cgtcttccat catcgccact ggttcaatat gaccacagcg
     1741 gcgaatgggg acctcgtccc tctggaaggc ggcgtcgtcg aattgagcgc cgatgacggc
     1801 attacctgga gcgacatcgg agcgtaagcg tcgcccggct acgatgctac gccgatcgtc
     1861 gcggatccca cgaatccact ggccggacgc cgtgcattcg caggacttag tcccggagcc
     1921 tcgttcgaat caccctccgc atcaccgttc acgactgtcc tcatcgacct cggcacgaag
     1981 tacgccggcc aacgcgtccg catccgcttc cgcatcggaa ccggaagcat caccgccttg
     2041 ccgctgggat ggcagatcga tgacgtcgca atcaacggaa tcacgaacct gccgttcgat
     2101 acgttcgtgg ccgaccgcgg cctatgcagt acatcgagct cgacgaccga gatccacgcc
     2161 ctcggagcgc tcctccaggc caccgtgtca tcgacgctgg ccacgccgaa cggcgccgta
     2221 gagttcttcg agaacggaaa aacggttggc gccgcgccgc tggtaaacgg cgtcgccgcc
     2281 tggaatccgg caacattcct cgcgccggga acgcacacga tcacagccag tttcggcggc
     2341 acgacgaatt tcacagcatc gacctccgcg cccgcgacga tcaccattgc agcccccacg
     2401 cgccggcgcg ctgcgcgata gttcaaacgg ctcggcggtt acccgtacta accgcgacgc
     2461 gatgaaggtg gtcgagtcgg cacacgaact gacggcagca tctgacagca gatgacttta
     2521 gtgtgtcggt cgttgaggac aaccgacgcg aacgggtaga cctcctggat cttttcgacc
     2581 agataacgcc gcagctcgcg gatacggagg actgccattg cctggatggc agatgacagt
     2641 tatattgtcc gtgctggcag acaggtgaaa gtcggctcca tgccgggtga tcgaggcctc
     2701 ggtctcgatc ctagcctgac tcaagcacta acttttccag cgtgatcgga ttcccctcca
     2761 agtaccactc accgtacgcg ttgcgccgct ggtcgtcgat atccatgatg aggcggtact
     2821 tcgaggcgtg cggcgcgaaa tagcgatcct cttttcgctc gaacattcgt tgaagagccc
     2881 acaggaagta gtcaatcact tgcagtccag cggagcggga aggttcactg ctcaggatct
     2941 tcgtcggcgg gaactgcctg tctccccatt tccttgcgaa gttcaggcgc gcgcgctcta
     3001 cggctgtcgc gatcgcggca gtgcgggttt tcgttcccct cttcgcgatg acgagctcat
     3061 tcgcctcgcc cagatgcaag cggtcgcggt gaagccgctt gagtaaatcg tcgtagatat
     3121 cgttctcgct gaccttcgtg tggatcgatt ggagttccag atgtggctcc aacttatccc
     3181 aactcaaacc ttaacggatt ccatcgctcc acgtgtaccg cccgcgcacc gacactcccg
     3241 gctcactcac gtcggtgcgc tatgtcacac atcgctgtta ttcaccagaa ttatgtggtc
     3301 aggcattccg cccgacacta tcttaggtac atcagtaaca gaagaagaag tatgaacctt
     3361 caagcgacga cgcgagaagc aaactcgaca cttcgagcag aaagcggcga ctatatcctg
     3421 taccaaaatc ttcaagcgtg gatagtgacc tccactggga gaacgtcaat cgcttccgac
     3481 cagctcgact cgttcgtcac gaaaatgaag cccacctatc cgggcgacag caaaagaagc
     3541 cccacaatca tcaggtatcg gcaagccgtt tcgcgaacct aaagggagat gatcatggcg
     3601 aagaaggaag ccgtaagggt cacgaagctt accctgcaga agaaggagac cgcgaacgtc
     3661 aaggcgggga tgacggttag gggaaacatc tgctgtacct gctctatgag cggcgtcgga
     3721 aagtccggta aacagtttgg cacgaccacg tcggctgcct agagcgcgct tctggatcac
     3781 cgttcggtag ctcgacgttg cgaacggtga tctccgggcg aagacggcgt gaaagggggg
     3841 cggcggttgt aaggctgatg ttccttgcga ccgg
//
LOCUS       DATLVW010000261         5662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_137812, whole genome shotgun sequence.
ACCESSION   DATLVW010000261 DATLVW010000000
VERSION     DATLVW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137812"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VNN25_08265"
     CDS             complement(<1..912)
                     /locus_tag="VNN25_08265"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXH91560.1"
                     /translation="MQKSLLLLLAAILFATTASADELSGADLDKRRKALADLLKEHWE
                     YTLRTSPEFASILGDRRYNDQLSDVSEAGAKRELAAAKKFLQRLDRIETTGFSEQETV
                     NKALLGHNLRDNIEDIELKEWEMPVTQISGIHLNAAQLPSVLPFATTKDYDDYAKRLR
                     GFPKQMDDTIANMRKGMRDHLMPPKFLLEKVVKQAEGIAAQEPDKTPFAGPLSKFPDA
                     IPEADRARLRTEIADAIRTSVLPAYVKFTAFVRDDYAPHGRKDVGMWALPEGTKRYAV
                     RVRQSTTTKLTADEIHQIGVHEVTRIEG"
     gene            1080..2462
                     /locus_tag="VNN25_08270"
     CDS             1080..2462
                     /locus_tag="VNN25_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HXH91561.1"
                     /translation="MSSSMSSKNVRTGCVLSLLVVVGLCAGSLALLSVGLESGWTGFL
                     AGFLMAAVPLPFYLAIAKLVDRFEPEPPWLLATAVFWGASTAILLAMIFNGIGEGIFA
                     ALLGQQQAAVMTPLFAAPFVEEIAKGFALLMLFLWKRDEFDNVTDGIIYAAMVGLGFA
                     MMENVQYYARAVTAGGGQAMGIFFVRGVLGPFCHPLFTSMTGIGFGIAQETNKKVVKF
                     IAPVLGLVGAMFLHFLWNLSTNLGLAFFATYLFFMVPMFFGVIIVAVFSLRRESIMIR
                     QHLESVVADHVLSHDDVVVVTSVRRRIAASSQALFSRGIGACIDRRRFHALASELAFH
                     SWRCSRFPVDDAEVIRAELIERVRAARAKLGLPIEIQPPAPQLVARLTLEIPLPDTIR
                     TRTLPDTIRTTPIRDTVNTRIADTVRTRPLRDTVRTRPLPETAYTSPIADPDRTIPDT
                     VRVPPLPDRD"
     gene            complement(2604..3014)
                     /gene="queD"
                     /locus_tag="VNN25_08275"
     CDS             complement(2604..3014)
                     /gene="queD"
                     /locus_tag="VNN25_08275"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HXH91562.1"
                     /translation="MPDVVELVKEFRFEAAHFLPHVPEGHKCRRMHGHSFRGEVVVRG
                     PIDPVTGWLIDFADLKRAIDPVVVRLDHYLLNEIEGLENPTSERLAIWFWQQLAPHVP
                     QLHRITIEETCSSRCHYYGGGESGVGSRSETGSE"
     gene            3065..3487
                     /locus_tag="VNN25_08280"
     CDS             3065..3487
                     /locus_tag="VNN25_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXH91563.1"
                     /translation="MARNSSETDTDRSLGTIIKELTEDITTLFRGEIALLKLEVKDTV
                     AKLGGGIGLFAGAVFVALFGLAFLFVTIMLVLVRLGVPAWLSALIVTVVLFATATVLG
                     LMGKKKFAAVEFVPSKSIESIKADVESIKSDIAQARGK"
     gene            3488..3772
                     /locus_tag="VNN25_08285"
     CDS             3488..3772
                     /locus_tag="VNN25_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91564.1"
                     /translation="MTDEMQQLDQTIAGARDRVGDRIDELDRRIRKQLDVKSLASEHA
                     TELVAGGAVIGFLVGFGFPKVVKRILQIGIPVTLIAMQVKKRSNGAGWMP"
     gene            complement(3808..3966)
                     /locus_tag="VNN25_08290"
     CDS             complement(3808..3966)
                     /locus_tag="VNN25_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91565.1"
                     /translation="MRKCKPRFNVDPETGELVEIDEELSAAIDEAAAQADRGEVIPWE
                     VFRKRWEK"
     gene            complement(4009..4173)
                     /locus_tag="VNN25_08295"
     CDS             complement(4009..4173)
                     /locus_tag="VNN25_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91566.1"
                     /translation="MRKRKPRFNVDPETGEMVEIDEELSAAIDEAAAQADRGEVIPWE
                     VFRKELFADD"
     gene            complement(4623..4760)
                     /locus_tag="VNN25_08300"
     CDS             complement(4623..4760)
                     /locus_tag="VNN25_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91567.1"
                     /translation="MDPETGEIVEIDEELSAAIEEAVASSARGESIPWEEFQKELFAD
                     D"
     gene            complement(4827..5114)
                     /locus_tag="VNN25_08305"
     CDS             complement(4827..5114)
                     /locus_tag="VNN25_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH91568.1"
                     /translation="MADAAERQLDEAHEWWEINRTAAPNAIRDDFEAMATKLASNPRI
                     GRRATNTRTPNVRQVFLKRVGYFIYYRIEGRPARLKVLAFWHSSRGKGPPI"
     gene            complement(5132..5290)
                     /locus_tag="VNN25_08310"
     CDS             complement(5132..5290)
                     /locus_tag="VNN25_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91569.1"
                     /translation="MSKPEPRFNVDPETGEMVEIDEELSAAIEEAAVQVARGESVPLE
                     VLLRRWKK"
     gene            complement(5314..>5662)
                     /locus_tag="VNN25_08315"
     CDS             complement(5314..>5662)
                     /locus_tag="VNN25_08315"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HXH91570.1"
                     /translation="AEQNREVFAVPGPIFSPGSEGTHRLIQYGAKLVHETDDILDELP
                     GDLRVPKAATPQPDSLLREVLRAFRRDEGTHIDNAAESLGRSVAAISEPILQLELGGW
                     LKALPGGRYVRVR"
BASE COUNT          948 a         1724 c         1755 g         1235 t
ORIGIN      
        1 gccttcgatg cgcgtcactt catgcacgcc gatctggtga atctcatccg ccgtaagttt
       61 cgtggtcgtg ctctggcgga cgcgaacggc gtagcgtttc gtgccttcag ggagtgccca
      121 catgccaacg tctttgcggc cgtgcggcgc gtagtcgtcg cgcacgaaag cggtgaactt
      181 tacatacgct gggagcacgg aggtgcggat ggcgtcggcg atctccgtgc gcaaacgagc
      241 gcgatctgct tccggtatgg catcggggaa cttcgacaac ggtccggcga agggcgtttt
      301 gtcgggctcc tgcgcggcga ttccttccgc ctgcttcacc accttctcca gcaggaactt
      361 cggcggcatc aggtggtcgc gcattccttt gcgcatgttg gcgatcgtgt cgtccatctg
      421 cttcggaaag ccgcgcaagc gcttagcgta atcgtcgtag tccttcgtcg ttgcgaatgg
      481 cagcaccgag ggcaactgcg cggcgttgag gtgaatgccg ctgatctgcg tgaccggcat
      541 ctcccactct ttgagctcga tgtcttcgat gttgtcgcga aggttgtgcc cgaggagggc
      601 tttgttgacg gtctcctgtt cggagaagcc ggtggtttcg atgcggtcga gccgctgcag
      661 aaacttcttc gccgcggcca gttcgcgttt cgcccccgcc tcggagacat cagagagctg
      721 atcgttgtag cggcgatcgc caaggatgga ggcgaattcg ggactggtgc ggagggtgta
      781 ctcccaatgt tctttgagga ggtcggcgag cgctttgcgg cgcttatcga gatccgcgcc
      841 gcttagctca tcggcggagg ctgtcgtggc gaagaggatg gcggccagaa ggaggagaag
      901 tgacttctgc atgggcggag cttaacgccg ccgggatcgg catggttgca aacttctgta
      961 atggtcgccg aagcaaaggc aaccacagag acacagagag cacagagatt atctctgtgg
     1021 cctctgtggt gaccggtttt tcaaattgac ccagtagatc gcgtaccatt tcaccgtcca
     1081 tgtcatcctc catgtcatcc aagaacgttc gcacggggtg cgtcctgtcg ctgctcgtcg
     1141 tcgtcgggct gtgcgctggc tcgctggcgt tgttgtcggt cggactggag agcggatgga
     1201 ccggatttct cgcaggcttt ctgatggccg cggtgccgtt gccgttttat ctggcgatcg
     1261 cgaagctggt cgatcgattc gagccggagc cgccgtggtt gctggcgacg gcggtgttct
     1321 ggggtgcgtc gacggccatc ctgctggcga tgatcttcaa cgggatcggc gagggaatct
     1381 tcgccgcgct cctcgggcag cagcaggccg cggtgatgac gccgttgttt gccgccccgt
     1441 tcgtcgaaga gatcgccaag ggatttgcgc tgctgatgct gtttctgtgg aagcgcgacg
     1501 agttcgacaa cgtcaccgac ggaatcatct acgcggcgat ggtggggctc ggcttcgcca
     1561 tgatggagaa cgtgcagtac tacgccaggg cggtcacggc cggcggcggt caggcgatgg
     1621 gcatcttttt cgtgcgcgga gttctcgggc cgttctgcca tccgctgttc acgagcatga
     1681 ccggcatcgg attcggcatc gcgcaggaga cgaacaagaa ggtcgtgaag ttcatcgcgc
     1741 cagtgttggg gctggtcggc gcgatgttcc tccatttcct ctggaacctc agcacgaacc
     1801 tcggcctggc gttcttcgcc acctacttgt tcttcatggt gccgatgttc ttcggcgtca
     1861 tcatcgtggc tgtcttctcc ctgcggcggg agtcgatcat gattcgccag cacttggaga
     1921 gcgtcgtcgc ggaccatgtt ctcagtcatg acgacgtcgt cgtcgtgacg tcagtccggc
     1981 gacggatcgc agcatcgtcg caagcgctct ttagtcgggg cattggcgcg tgtatcgacc
     2041 ggcgccgctt tcatgcgctg gcaagcgagc tggcattcca ctcctggcgt tgcagccgct
     2101 ttccggtcga tgacgccgag gtcattcgcg ccgagctgat cgagcgcgta cgcgccgcgc
     2161 gtgcgaagct cggtctcccg atcgagattc agccgccggc accgcagctc gtggcccgtt
     2221 tgacgctcga gatcccgctt ccggacacga tccgaacccg cacgttgccg gatacgatcc
     2281 gcaccacgcc gattcgggac accgtcaaca cgcgaatcgc ggatacggtt cggacccggc
     2341 cgctgcgcga cacggtccgc acccgcccgc tgccggaaac ggcatacacc agtccgattg
     2401 cggatcccga tcgcacgatt ccggataccg ttcgcgtgcc gccgctcccg gatagggact
     2461 gagcgtttgc gcgggggtcg cggttcgggg gtcgggggtc ggagaatctt gtttaaatca
     2521 gtcgcaacat ccggctgttc tgcacctgtg ctcggacccg tgtcatcagc ctgtcgtggt
     2581 tgacgcgtcg ccggatctgc gactcattcc gaacctgtct cgctccgact cccgaccccc
     2641 gactccccac ccccgtagta atggcacctc gagctgcacg tctcctcgat cgtgatgcga
     2701 tgcaactgcg gcacgtgcgg cgccagttgc tgccagaacc agattgccag ccgctccgac
     2761 gtcgggttct cgaggccttc aatctcgttg aggaggtaat gatcgagccg gaccacgacc
     2821 gggtcgatgg cgcgtttgag gtcagcgaag tcgatcagcc acccggtgac gggatcgatc
     2881 ggtccgcgca cgaccacttc gccgcgaaac gagtgtccat gcatgcggcg gcacttgtgt
     2941 ccctcgggca catggggtag aaagtgcgcc gcctcaaagc gaaactcctt caccagctca
     3001 acgacgtcgg gcatcggcgg cgattgtagt ggcgaggcac ctcttttgct cctatcggcg
     3061 caatatggct cgaaatagtt cagagacgga cacggaccgc tcccttggaa cgatcatcaa
     3121 ggagctgacc gaagacatta cgacgttgtt ccgtggcgag atcgcgctgc tcaagctgga
     3181 ggtcaaagac acggtcgcga agctcggggg cggcatcggg ctgtttgccg gtgcggtgtt
     3241 cgtggcgctc ttcggactgg ccttcctctt cgtcaccatc atgctcgtgc tcgtccgcct
     3301 cggggtgcca gcctggcttt cggcgctgat cgtgacggtg gtcctttttg cgacggcaac
     3361 cgtcctcggg ctcatgggga agaagaagtt cgccgcggtg gagttcgttc cgtcgaaatc
     3421 gatcgagagc atcaaggccg acgtggaatc gatcaagagc gacatcgcgc aggcaagggg
     3481 caagtagatg accgacgaaa tgcagcagct cgatcagacc attgccggcg cgcgcgaccg
     3541 cgtcggcgac cgcatcgatg agctcgaccg gcgcatccgc aaacagctcg acgtcaagag
     3601 cctcgcttcc gaacacgcca ccgagctcgt agccggcggc gccgtgatcg gcttcctggt
     3661 cggtttcgga ttcccgaaag tcgtgaagcg aatcctccag atcggcatcc cggtcacgct
     3721 gatcgcgatg caggtgaaga aacggagcaa cggcgcgggg tggatgccgt agcggcacgg
     3781 ctctacgagt cgtagatcgc tgatcgccta tttttcccag cgcttccgga agacttccca
     3841 gggaatcact tcgccgcgat ccgcttgcgc ggctgcctcg tcgatggccg cactgagctc
     3901 ttcgtcgatt tccaccagtt cgcccgtttc ggggtctacg ttgaatctcg gtttgcattt
     3961 gcgcatgacg atgactttac ctcggtgcga ggcggttcaa ttcgctcgct agtcgtccgc
     4021 gaagagttcc tttcgaaata cttcccaggg aatcacttcg ccgcgatccg cttgcgcggc
     4081 tgcctcgtcg atggccgcac tgagctcttc gtcgatttcc accatttcgc cggtttcggg
     4141 atccacgttg aatctcggtt tgcgtttgcg catgacgatg actgtacctc ggtgcgaggc
     4201 gctacaacag gatgactaga ttggtggccc ctttccgcgt cttacgtgcc acagagctat
     4261 cacttcgagc tctcggaatc gccacgcagt tcacgcgcca ccatgccttg gctgcggcga
     4321 tctggcgttt tgcggtcggt tgagtctcaa tattcgtagc gcggtttttc agatctcgac
     4381 ccgtttcaaa ctttaaggct atctggccgg attcaaccac cgaggcggga cgcctcgatg
     4441 gccgcgggcg agacgccagg cgagacgccc acactcctca ggcgctcgag aatttgcaga
     4501 ggagtgcggt cgtctcgacc gcgggccctc gggcgtcctc gcccgagggc ggtgagggcg
     4561 gaggtcggtg tttcgtcgac gttcttgttt gccctgaagt acagcttctg cagcgcaaac
     4621 tcctagtcgt ccgcaaagag ctccttctgg aactcttccc atggaatcga ttcgccgcgc
     4681 gccgacgacg cgactgcttc ctcgatggca gcgctgagct cttcgtcgat ttccactatt
     4741 tcgcctgttt cgggatccac gttgaatctc gtttcgttgc tcacgacaaa actctacctc
     4801 ggcgagccga caaaacgtga cgaggattag atcggtggcc ccttgccgcg gctcgagtgc
     4861 cagaaagcca gaaccttcag acgtgctggc cgcccctcga tacggtagta gatgaagtag
     4921 ccgacccgtt tcagaaacac ctgacgcacg ttaggtgtcc tcgtattcgt tgcgcgtctt
     4981 cctatcctgg ggttgctcgc gagcttggtc gccatcgctt cgaaatcgtc ccggatcgca
     5041 tttggtgctg ccgtccgatt gatctcccac cattcgtgtg cctcgtcgag ctgccgttcc
     5101 gcggcatccg ccacgtcgac tgggagagga actacttctt ccagcgcctc aacagcacct
     5161 ccaagggaac gctttcgcct cgagccactt gcaccgccgc ttcctcgatg gccgcactga
     5221 gctcttcgtc gatttccacc atttcgcccg tttcgggatc cacattgaac cttggctcgg
     5281 gtttgctcat gccggaattc tacctcggag acgctagcgc acgcggacat agcggcctcc
     5341 tggcagcgct ttcagccacc ctcccagttc gagctgaaga atcggctcgg agatcgcggc
     5401 gaccgagcgg cccagcgact cggcggcgtt atcgatgtgc gttccttcgt cccggcgaaa
     5461 tgcgcgaagc acctcgcgca agagagagtc gggttgcggc gttgccgctt tcggaacacg
     5521 gagatcgccg ggcagttcgt cgaggatgtc gtcggtctcg tgaaccaact tcgctccgta
     5581 ttggatcagg cggtgcgtcc cttccgagcc gggagaaaag atcggacctg gcacggcgaa
     5641 gacttcgcgg ttctgctccg ct
//
LOCUS       DATLVW010000262         3478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_178709, whole genome shotgun sequence.
ACCESSION   DATLVW010000262 DATLVW010000000
VERSION     DATLVW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178709"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1369
                     /locus_tag="VNN25_08320"
     CDS             164..1369
                     /locus_tag="VNN25_08320"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HXH91571.1"
                     /translation="MLLHDESPAAVEEREKAFILGTYARTTLHPRSGKGARITDADGR
                     EYWDLLGGIAVNALGHRHPRLVKALRDESNALLHLSNLYYHPAQGLLAERLVRASGLC
                     RAFFCNSGTEANEAALKFARLANPGKPGVVALNESFHGRTLGSLSMTGHEAYRKPFEP
                     LMPGVTFVEPNDVAALEAAVDETTGAIFLEPVMGEGGVIPLTHEFLAAARRVADRTGA
                     ILVFDEIQCGLGRTGTLFAFQQSGIVPDIITLAKPLGGGLPLGAVITGPAIEGLVKPG
                     HHGTTFGGNPVACRLGLAVLDEIEDSKLLERINTLGAWFGTQLAKLKKRNPSIVEVRG
                     SGLMWGIELDGPAKPVVAELLAKGFVVGSARENVIRLLPPYVTPKKAFAEFVEALEQI
                     LGKLERKVA"
     gene            1401..2666
                     /locus_tag="VNN25_08325"
     CDS             1401..2666
                     /locus_tag="VNN25_08325"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXH91572.1"
                     /translation="MTHFKKVALAYSGGLDTSIIIPWLKENYGCEVVAVAVDVGQAEE
                     TSGLAEKAYQTGACDFHLIDAREEFARDYLFPVLKAGAIYEKDYLLGTSTARPLIARK
                     QVEIALATGCDALAHGCTGKGNDQVRFELAYQALAPELAVIAPWREWSIISREDAIDY
                     ASSRGIPVPVTKKDPYSRDRNLWHLSHEGGPLEDPSFEPEPSMFKLSVDPIEAPNTPE
                     KVAITFVAGVPIAVNGEAMSPGALISKLNEIGGRHGVGRIDIVENRLVGIKSRGVYET
                     PGGTLLVNAIKALESITLDRDSAHEKERIGTRYAELVYFGQWFSPLKEAFDAFVDALA
                     EAVTGTVTLKLYKGNAIVIGRTSPNSLYSEALASFNMTGYDARDAAGFIRVFGLPTRG
                     RYRIEQHNRLDDTNPGDEVTAARLRKAAG"
     gene            2663..>3478
                     /gene="argH"
                     /locus_tag="VNN25_08330"
     CDS             2663..>3478
                     /gene="argH"
                     /locus_tag="VNN25_08330"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXH91573.1"
                     /translation="MTQKLWGGRFVSEPSQLLRRLNDSFAFDRELFDEDVEGSIAWAR
                     AIAEAGVLSEEELDAILGALARIEAPADGDYEDVHSYVEASLYELIGAGAGKLHTGRS
                     RNDQVATDLKLWLKRACDEAIEIVNALAATLATRALEEAATPMPGYTHSKQAEPVTFG
                     HWCLAYVEMLRRDRLRFVSAAERGDECPLGSGALAGTPLAIDRDALARSLGFARSNAN
                     SLDGVSDRDFAAEYLFASSMMLTHLSRMAEDLIFFTSDEASYAELPDAMATGSS"
BASE COUNT          661 a         1072 c         1121 g          624 t
ORIGIN      
        1 gagaccggaa acgtcgtttc cgaactacac gcaccggaag cggaagcgct gcttggcagc
       61 gaagtcgtca ccggaggcat gcgcccgaaa ctgcaggctg cgttgcacgc actcgaatcg
      121 ggcgtgcaac gaatcacgat cggagaagaa ggaggtacga accttgttgc tgcatgacga
      181 atcgcccgcc gccgtcgaag agcgcgagaa ggccttcatt ctcggcacct acgcacgcac
      241 gacccttcat ccgcgcagcg gcaaaggtgc gcgcattacc gacgccgatg gccgcgaata
      301 ctgggacctg ctcggcggca ttgccgtcaa cgcgctcggc caccgtcacc cgcgcctcgt
      361 gaaagcgttg cgcgatgagt cgaacgcgct gctgcacctc tccaatcttt actatcaccc
      421 ggcgcagggt ttgctcgcgg agcgcctcgt gcgcgcctcg ggactctgcc gcgcgttttt
      481 ctgcaacagc ggaacggaag cgaatgaggc tgcgctgaaa ttcgcgcggc tcgccaatcc
      541 cggaaaacct ggagtcgttg cattgaacga gagctttcac ggacggacgc tgggatcgct
      601 gtcgatgacc ggacacgagg cctatcgcaa gccgttcgag ccgctgatgc cgggcgtgac
      661 cttcgtcgag ccgaatgatg ttgccgcgct cgaagccgca gtcgatgaaa cgaccggcgc
      721 gatttttctc gagccggtga tgggcgaggg tggcgtcatc ccgctcacgc acgaattcct
      781 cgctgcggca cggcgcgtcg ccgatcggac gggcgccatc ctcgtcttcg atgagattca
      841 gtgtggcctc gggcgcaccg ggacgctctt cgcgtttcag cagagcggca tcgttcccga
      901 catcatcacg ctggccaaac cgctcggcgg cggcctgccg ctcggcgcgg tcatcaccgg
      961 tcctgctatc gaaggcctcg tgaagccggg acatcacggc accacgttcg gcggcaatcc
     1021 agtcgcctgc cgtctcggcc tcgcagtgct cgacgagatc gaagactcga aacttctcga
     1081 gcgtatcaac acactcggcg cgtggttcgg aacgcaactc gccaaactca agaaacgtaa
     1141 cccatcgatc gtcgaagtgc gcggctcggg cctcatgtgg ggcatcgagc tcgacggacc
     1201 ggccaagccg gtcgtcgcgg agttgcttgc gaaaggcttc gttgtcggca gcgcgcgcga
     1261 gaacgtcatc cgactgctgc cgccgtacgt cacgccgaag aaggcattcg ccgaattcgt
     1321 cgaagcactg gaacagatcc tcggaaaact ggaaagaaaa gtcgcgtagc agatcgcaga
     1381 cagatccaaa ggaaacatca atgactcact tcaagaaagt cgcactcgcc tactcgggcg
     1441 gactcgatac ctcgatcatc attccctggc tgaaggaaaa ctacggctgt gaagtcgtag
     1501 cggttgccgt cgacgtcggc caggccgagg agacgtcggg gcttgcggag aaggcgtatc
     1561 agaccggcgc gtgcgatttc catctcatcg atgcgcgtga agaattcgcg cgcgactacc
     1621 tgtttcccgt cctgaaggcc ggcgcgattt acgagaagga ttacctcctt ggcacctcga
     1681 ccgcgcggcc gctcatcgcg cgcaagcagg tcgagatcgc gctcgccacc ggctgtgacg
     1741 cgcttgcgca cggctgcacg ggcaagggca atgatcaggt tcgcttcgag ctcgcctatc
     1801 aggcgctcgc gccggagctg gcggttatcg cgccgtggcg cgagtggtcg atcatctcgc
     1861 gcgaagacgc gatcgattac gcgtcgtcac gcggcatccc ggtgcctgtg acgaagaagg
     1921 atccgtactc gcgcgaccgc aatctctggc atctctcgca tgagggcggg ccgctcgaag
     1981 atccgtcgtt cgagccggag ccgtcgatgt tcaagctgtc agtcgatccg atcgaggcac
     2041 cgaacacgcc ggagaaggtc gcgatcacgt tcgttgctgg tgtaccgatc gcggtcaatg
     2101 gcgaggcgat gtcacctggc gcactgatct cgaaactgaa tgagatcggc ggacgtcacg
     2161 gtgtcgggcg tatcgacatc gtcgagaacc gtcttgtcgg catcaagtcg cggggcgtgt
     2221 acgaaacacc cggcggcacg ctgctcgtca atgcgatcaa agccctcgag tcgatcacgc
     2281 tcgaccgcga cagcgcgcac gagaaggagc gaatcggcac gcgttacgcg gagcttgtct
     2341 acttcggcca gtggttctcg ccgctgaagg aagcgttcga tgcgttcgtc gatgcgctcg
     2401 cggaagcggt caccggtacc gtcacgctga agctgtacaa aggtaacgcg atcgtcatcg
     2461 gccgcacgtc gccgaactcg ctgtattccg aggcgctggc gagcttcaac atgaccggat
     2521 atgacgcgcg cgatgcggcc ggattcattc gcgtgttcgg actgccgacg cgtggccgtt
     2581 atcgcatcga gcaacacaac cgtctcgatg acacaaaccc gggcgatgaa gtcaccgccg
     2641 cgcggctgcg gaaggcggct ggttgacgca gaaactgtgg ggcgggcgct tcgtgtcgga
     2701 gccatcgcag ttgctgcggc ggctcaacga ctcgtttgcg ttcgaccgcg agttgttcga
     2761 tgaggacgtc gaaggttcga tcgcatgggc gcgcgcgatc gcggaggccg gcgtgctgtc
     2821 cgaggaggag ctggacgcta ttctcggcgc gctggcgcgg atcgaagcgc cggctgacgg
     2881 cgactacgaa gatgtgcact cgtacgtcga ggcgagcctc tacgaattga ttggagcggg
     2941 cgcgggaaaa ctccacaccg gccgttcgcg caacgatcag gttgcgacag atctaaagct
     3001 gtggctcaag cgcgcgtgcg acgaagcgat cgagatcgtg aacgcgctgg cagcgacgct
     3061 ggcaacccgt gcgctcgaag aagcggccac gccgatgcct ggctacacgc actcgaaaca
     3121 ggccgagccg gtaacgttcg gacactggtg cttggcgtat gtggagatgc tgcgccgcga
     3181 ccgcttacgc tttgtcagtg ctgccgagcg cggcgatgaa tgcccactcg gctccggcgc
     3241 gctcgccggt acgccgctgg cgatcgatcg cgatgcgctg gcgcgctcgc ttggatttgc
     3301 gcgctcgaac gcgaactcgc tcgacggtgt ttcggatcgc gatttcgcgg cagagtattt
     3361 gtttgcgtcc tcgatgatgc tgacgcactt gtcgcgcatg gctgaggacc tgatcttctt
     3421 taccagcgac gaagcgtcgt acgccgagct tcccgacgcg atggccacag ggtcgtcg
//
LOCUS       DATLVW010000263         4797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_589308, whole genome shotgun sequence.
ACCESSION   DATLVW010000263 DATLVW010000000
VERSION     DATLVW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589308"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VNN25_08335"
     CDS             <1..851
                     /locus_tag="VNN25_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH91574.1"
                     /translation="LPAYIFVMRNVLLATWIGLAAVTALGQTTTQTKPQYTDVFYRSG
                     SLRIEAYLYKPDGAGPFPVVIYNHGSREGHERDSVPFAYIGSMLTRAGYVVLVPERRG
                     YGKSDGAMWRDETQRDPARLVPRLQEEVDDVLAGIDYLRTLPFADTKRMGIMGWSFGG
                     IVTMLATSRSSSFLVAVDQAGGSLSWDGNPSVRSALVGAAGKSTTPTLFLVAKNDRTT
                     AAVTTVASIFEKRHLPHRLVVYEPFNPPVAGHADAPGHLIFSMYGASIWESDVVQFLD
                     RYLKKG"
     gene            complement(1685..2776)
                     /locus_tag="VNN25_08340"
     CDS             complement(1685..2776)
                     /locus_tag="VNN25_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXH91575.1"
                     /translation="MSSNPFPAATPAAPEADEAPPIAPRPRPSNEEIAAWLLTAALLG
                     YVLVWHLVPAVVGGLVLYLVLDRLSQSFSRRMPGAAARPLALILVTLVGGGVIFGVVA
                     LLVSYLRHHVDNLPAMMTRMADILQSTRAWLGDYGEQLIPEVLTDADTFKAALADWLK
                     QHAETLKLAGSTVSLGLVHLFMGMLLAVMVFFRHVTHHDGRAPGTLSEMLEQKVVRFT
                     RAFAQIATAQVKISVLNTALTGLYLLVVLPLFGQHIPFATTIVFVTFVCGLIPVLGNL
                     ISNSVIVILSLGISPGTAAASLLFLIIIHKLEYLTNSRIVGSQTDSQAWEILLAILIG
                     ETAFGIGGVVMAPILYAFVKRELRERGLV"
     gene            2841..3170
                     /locus_tag="VNN25_08345"
     CDS             2841..3170
                     /locus_tag="VNN25_08345"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXH91576.1"
                     /translation="MTWVRTEKSNMDKRVFKHRGEFALRQVGNESILVPIRNRVGDLD
                     SVYVFTPVAARIWSLLDGVRDVDAVVDTICDEYDAESAVVITDLQELLGSLRGADLIA
                     ETAETNS"
     gene            3167..4429
                     /locus_tag="VNN25_08350"
     CDS             3167..4429
                     /locus_tag="VNN25_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH91577.1"
                     /translation="MTTIETGTYTDFSTIIHRQVAARVPLDGTIEVTNRCPLACAHCY
                     NNLPMDDLAARVRELSLEEYQHLLDELSEQGCLWLLFTGGEIFARRDFLDIYTYAKRK
                     GFLITLFTNGTLITEKIADFLVELPPFVIEITLYGATRETYEELTRVPGSYDKCIRGI
                     DLLLERKLPLKLKTVAVSINKHEIPAMKQMAADRGVEFKFDAMINPRIDCSSSPLAVR
                     LTPSEIVTFDLEDTQRASEWKRLAKDFGAPAYVEGEERHVYDCGGGLTGFAIDPYGDL
                     SICVLSHKDKYNVRDGNFREGWTDYLRGVRARKITRPTKCTGCGLKTMCGMCAANGEL
                     ENNDAESPVDFLCQVAHLRAMTLDVPVRPHGECEYCIGGAEHAIVRDAAEALKLAEAE
                     RIAAIPIAPDEAAASCGSGGCSSCSTAG"
BASE COUNT          956 a         1439 c         1577 g          825 t
ORIGIN      
        1 ctctcccggc gtatattttc gtgatgcgaa acgttcttct ggcgacgtgg attggtcttg
       61 cggcggtgac ggctctcggt cagacaacga ctcagacgaa gccgcagtac accgacgtct
      121 tctaccgaag cggatcgctg cggatcgaag cctatctgta caaaccggat ggtgccggtc
      181 cgtttcccgt cgtcatctac aaccacggct cgcgtgaggg gcacgagaga gactccgttc
      241 ccttcgccta catcggttcg atgctgacgc gcgccggata cgtggtcctg gtgccggagc
      301 gccgcggcta cggcaagtcg gatggcgcga tgtggcggga tgagacgcag agggatccgg
      361 cgcgcctcgt gccgcgactg caggaggaag tggatgacgt gcttgccgga atcgactatc
      421 tgcgcacgct gccgttcgcc gatacgaaac gaatgggcat catgggttgg tcgttcggtg
      481 gaatcgtgac catgctggct acgagccgca gttcctcgtt tctggttgcg gtcgatcagg
      541 ctggcggatc gttgtcgtgg gacggcaacc cgtcagtgcg cagcgccctg gtgggcgccg
      601 ccgggaaatc gacgacgccg acgctgtttc tcgtggcgaa gaacgatcgt acgaccgcgg
      661 cggtgactac ggttgcctcg atcttcgaga agcgacacct tccgcaccgg ttggtggtct
      721 acgagccgtt caatcctccg gtcgccggac acgccgatgc accggggcac ctcatcttct
      781 ccatgtacgg cgcgagcatc tgggagagcg acgtcgtcca gttcctggat cggtatctga
      841 agaaaggtta gcccgcactt ctcacacttg caggagattg gcggcagcgc gttgctgcaa
      901 cgtgtgagac gtgcggagta gaggtggcca ccgacagaac gtcgtcatcc ttcgtggcga
      961 cagcgctaca ccctcggcgc gcacatgctg cacccggcgc gcagcaacct tgcgcccctg
     1021 ggtacagcgt tgcgccatcg ggccgcagtg cggacacggc gtacggcaac gcggacaact
     1081 agcgccgcac gtgcggacag gaagagccgc agtgcggaca actagcgccg cacgtgcgga
     1141 caggaagagc cgcaatgcgg acaactagcg ccgcacgtgc ggacagggag cgccgcaatg
     1201 cggtcaacta gcgccgcacg tgcggacagg aagcgccgca atgcggacaa ctagcgccgc
     1261 acgtgcggac aggaagagcc gcaatgcggt cagcttgcgc cgcacgtgcg gacaggaagg
     1321 gccgcaatgc ggacaactag cgccgcacgt gcggacagga agcgccgcaa tgcggacaac
     1381 tagcgccccg cgtgcggaca ggaagcgccg caatgcggat aactagcgcc ccgcgtgcgg
     1441 acaggaagcg ccgcaatgcg gacaactagc gccgcacgtg cggacaagaa gcgccgcaat
     1501 gcggacaact agcgccgcac gtgcggacag gaagcgccgc aatgcggtcg ccctcgggtg
     1561 cgatggcgcg cgcccatatt gcggcagccc ggcccgtcag tgcccttgac atcacgtctc
     1621 atcggcaagc aacaaggaag aggtgtagca agaaggggac accgagtgtg gaagaaaacg
     1681 cggttcagac cagtccgcgc tcgcgtagct cgcgtttgac gaacgcgtag agaatcggcg
     1741 ccatcaccac gccgccgatg ccgaacgcgg tctcgccgat gaggatcgcc aacaggatct
     1801 cccaggcctg cgagtcggtc tggctgccga cgatgcgcga gttcgtcagg tactcgagct
     1861 tgtgaatgat gatgagaaac agcagggagg cggcggcggt gccgggggag atgccgagcg
     1921 agagaatgac gatcaccgag ttggagatga gattgccgag gactgggatc aagccgcaga
     1981 cgaaggtcac gaagacgatc gtcgtcgcga acggaatgtg ctgtccgaaa agaggtagca
     2041 cgacgagcag atagagcccg gtcaacgcgg tgttgaggac cgagatcttc acctgggccg
     2101 tcgcaatctg cgcgaacgca cgcgtgaagc ggaccacttt ctgctccagc atctcgctga
     2161 gcgttccggg cgcgcggccg tcgtggtgcg tgacgtggcg gaaaaagacc atcacggcga
     2221 ggagcatgcc catgaacaga tggaccaagc caagactgac ggtgcttccg gcgagcttga
     2281 gggtttcggc gtgctgcttg agccagtcgg cgagggcagc cttgaacgtg tccgcgtcgg
     2341 tcagcacctc ggggatgagt tgctcgccgt agtcgccgag ccaggcgcgg gtcgactgga
     2401 ggatgtcggc catcctggtc atcatcgcgg gaaggttgtc gacatgatgg cgaaggtagg
     2461 agacgagcag cgccaccacg ccgaagatca cgcccccgcc gacgagcgtg acgaggatga
     2521 gagccaacgg tcgcgccgct gctcccggca tgcgccgcga gaacgactgt gagagcctgt
     2581 cgaggacgag atagagcacg aggccgccca cgaccgcagg cacgagatgc cagacgagga
     2641 cgtagccgag gagcgcggcc gtcagcagcc aggccgcgat ttcttcgttc gacggacgcg
     2701 gccgcggagc gatcggcggc gcttcatctg cttcgggcgc cgcaggcgta gcggcaggga
     2761 acggattcga gctcatcggt ttttgattgt agactctggg tcaaataatt cggttgccgg
     2821 acgcacatga gcatgtgaca atgacgtggg tcagaaccga aaaatcgaat atggacaaac
     2881 gcgttttcaa acatcgcggt gagtttgctc tgcggcaggt gggcaatgag agcatcctcg
     2941 tgcctatccg caatcgtgtc ggcgacctcg actctgtcta tgttttcacg cccgtggcgg
     3001 cccggatctg gtcgcttctt gacggcgttc gcgatgtcga tgctgtcgtg gacacgatct
     3061 gcgacgagta tgatgccgaa tccgccgtcg tgatcaccga ccttcaggag ctactgggct
     3121 ctctccgcgg tgcggatctc atcgctgaga cggccgagac aaactcatga cgacaatcga
     3181 gacgggcacc tacaccgatt tcagcaccat catccaccgg caggtggcgg cgcgcgtgcc
     3241 tctcgacggc acgatcgagg tcacgaatcg ctgcccgctg gcttgtgcgc attgttacaa
     3301 caatctcccg atggacgacc ttgccgcgcg cgtccgcgag ctttcgctgg aggagtatca
     3361 acaccttctc gacgagctct ccgaacaggg atgcctctgg ctgctgttca cgggtgggga
     3421 gatcttcgcg cggcgcgact tcctcgacat ctacacctac gcaaagcgga agggcttcct
     3481 tatcacgctc ttcacgaacg gtaccctcat caccgagaag atcgccgatt tcctcgtcga
     3541 actgcctccg ttcgtcatcg agatcaccct ctacggtgcc acgcgcgaga cgtatgaaga
     3601 gctcacgcgg gttcccggct cgtatgacaa gtgcattcgc ggcatcgatc tgctgctgga
     3661 gcggaaactt ccgctgaagc tgaagaccgt ggccgtctcc atcaataaac acgagatccc
     3721 tgccatgaag cagatggctg ccgaccgcgg cgtcgagttc aagttcgacg ccatgatcaa
     3781 tccccgtatc gactgttcgt ccagtccgct ggccgtgcgg ctcacaccgt cggaaatcgt
     3841 gacctttgac ctcgaggata cgcagcgcgc ctccgagtgg aagcggctgg caaaggattt
     3901 cggcgccccc gcgtatgtcg aaggcgaaga gcgccacgtc tacgactgcg gcggcggtct
     3961 taccggcttc gccattgacc cctacggcga cctctccatc tgcgttctct cgcacaaaga
     4021 caaatacaat gtgcgcgacg ggaacttccg cgagggatgg acggattacc tccggggcgt
     4081 gcgcgcgcgg aagatcaccc ggccgaccaa gtgcacgggc tgcggtctga agacgatgtg
     4141 cggtatgtgc gccgcgaacg gcgagctgga aaataacgat gccgagtcgc ctgtcgattt
     4201 tctctgccag gttgcgcatc tgcgcgcgat gacgctcgac gttcctgtaa ggccgcacgg
     4261 cgaatgcgaa tactgcatcg gcggagcgga gcacgcgatc gttcgcgatg ccgccgaggc
     4321 tctcaaactc gcggaagcgg agcgcatcgc cgcgattccc atagcgccgg atgaggcagc
     4381 ggcaagttgc ggctccggag gttgttccag ttgttcgacc gcgggctgat cgctcgctga
     4441 aagaggagaa cgatgcgaaa gtcggaaacc aacgccagaa agcggtacga gaagccggag
     4501 gcgaaacgct tccctatgcg tccggacgaa gcagtcctgg ggggctgcaa gaccaacgga
     4561 acctccgggc cgggcggtgg gggggtgtgc aagcagttcg gcaatccctg ctctggtcag
     4621 tcctcatagc gtgaaatgtc gtccgcgcct gacctgatcc gtatcgccgg aatcactctt
     4681 cgcgtccgtt acgccgacga gtcgttgcga cggctgctgc cggccacggt ggggcgtttt
     4741 gcgatcgatg ccgatgacgg cccggatctc gacctcttcg tggaggcgat gcccgag
//
LOCUS       DATLVW010000264         6273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_258967, whole genome shotgun sequence.
ACCESSION   DATLVW010000264 DATLVW010000000
VERSION     DATLVW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258967"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VNN25_08355"
     CDS             complement(<1..438)
                     /locus_tag="VNN25_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH91578.1"
                     /translation="MIRKKQKGFTLIELLIVVAIIGILAAIAIPNLLTAMARSKQKRT
                     MADMRTIATAWEARATDVNKYSAAGACSIPSTSSSPSQMSGMLAPTYIKVLPPKDGWN
                     TGWEYRVGNQTYAIISYGKDQTANPSYGTCGPTTNFDCDIVYAE"
     gene            complement(591..2000)
                     /locus_tag="VNN25_08360"
     CDS             complement(591..2000)
                     /locus_tag="VNN25_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH91579.1"
                     /translation="MRRWKWLLVSVAAGLAFIGGLLIYNKQTNEDINSQLWIPKPLTI
                     TPAIALLQQYVRIDTSNPPGKELAGARFLAATLAKNGIPAEIIESAPGRANVYARLSG
                     KQHESGLLLLNHIDVVPADKEGWTRPPFGAEFRLNQLYGRGTLDMKGIAVCELLAFID
                     LARAHRTPAHDIVFLATADEETGGGMGVAWLLAHRPDVFAGIRYALNEGGITETTREH
                     ITYFGIETGTKMMIKVQLHAPDRQSMQRLRIALEPFMTPADPERVLPEAKEFMHDLAP
                     LRIEQGQYLDDINRTIAEGKFWLLPTGYRELTQNIVYPATIEPDGGSGVSMYVNLYNL
                     PDENPDSRIEWLRGVTSRYGVTIAQVIRKSGPAPISSRHTPMFALIASEASKQYPGTR
                     TGSEILVATTNDSKYLRAKGVDAYGLMPFPTDWYQTQGIHGIDERVRADWFDSGVNLM
                     RNITQRYAFGQESEGLSRP"
     gene            complement(2005..4536)
                     /locus_tag="VNN25_08365"
     CDS             complement(2005..4536)
                     /locus_tag="VNN25_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91580.1"
                     /translation="MSDKPPIPVSRRTDLLALGLLFLIATICFADVLAGINQLYMRDM
                     TRYYYPAKQILRDVAQHGEFPYWNRYFSAGQPLAANPEHEVFYPLTWLILLPSYDLGY
                     RLLVLIHIYIALFGMYALLRSMDLRPPAAWMGALSFGLGGLFLSYINLLPILFCAAWI
                     PLTCLYVRRFLLRRNMRDFAAAALFLGIQFLVAEPTTVMQTGLLIGMYGLYRGWYSRP
                     RASKLITRVVWIAFISATGFLLGAAQILPAIDHVRDSARSRPFDFDLVSAWSMPWAKF
                     AELIYPNILGHISIENRMYYWAGGLYPGMASPFLFSIYVGIAVIALAIGGAFARPRGG
                     RLVLIIVFFSSLLAVGGHTPLYHWLYDAGIATSIRYPEKFILMAVFALVLFASQMLDR
                     MLDGDDDVRSGALGFAAASTLVAAGIAVSAFTRFYPRLFILLGKINDLSSIRKAWAQT
                     AGAGLSYPLLLSRTDWVFATLRGLILIALLLMVRKRRRRVWLAAAALFVTIDLAFVTY
                     ELNPREPRRFFDLPRAAAALPPNRQDYRVFHEADWYGTEKPAQQYFSTGEAVYWVVRN
                     GLFPMTPAAARVQTVLERDYDKTSLLPTVDLVDSVWDVKRSGRAAWWKPFMAMSNAWY
                     RATFRDFEREKKRNHGNFKESLPIGFEEGPHYPRYYFSNQIITIRDREDFVKQLSTWT
                     FSPLVAFIPRPSFVPAAGVVSHVVETANTVNLDAECGGQGFLVMSVTPHKYWTIRVDG
                     SRVPAITTNIGYQGIILTPGHHHVEMVYRNELVRVGMLISLITGAFLIGILIVYRKPR
                     DTAAALPAYEERIHVVADADGTHVEPAAVETAGVEGSTDDDGLVG"
     gene            4599..5381
                     /locus_tag="VNN25_08370"
     CDS             4599..5381
                     /locus_tag="VNN25_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH91581.1"
                     /translation="MPHHEKKVVAVLPAYNAEKTLQATFDDIPADWVDEILLVDDRSR
                     DRTVELARQLPMRVVVHEHNRGYGGNQKTCYRTAMDEMGGEIMVMVHPDHQYDPKIIP
                     QLVQPLLRGDCEAVFGSRMLGGRPMEGGMPKWKYLANLFLTMVENATFYIFLSEYHSG
                     FRAYTKRYLDTVNFEANSDNFVFDTEIIAQGVAKGMRIREVPISTRYFDEASQIGFWR
                     SVRYGLEILKTMVLYKLHKKHLYSHRIFRDPVPRGTVRTATT"
     gene            complement(5362..>6273)
                     /locus_tag="VNN25_08375"
     CDS             complement(5362..>6273)
                     /locus_tag="VNN25_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91582.1"
                     /translation="GLLAQRSWGRLARVVAGGAPFALMLALYQKLAFGSVLASPYTYE
                     KLPEYRALAHTGLFGLQAPSLEILMRLLFDPARGLLIFAPIIAAALLAIPAAKRALPR
                     AAFVTLLLTPAALIAIYSGYPNWHGGWNVGPRYIAGAIPFLLFPLAFARGRRIAAILL
                     GASAAAVVPITLTFPFPDRSFIMPWSTLALPLLRDGLVAPNLLHLVWRPLAIIVPLAI
                     VAAAIIIATRRNALLAALGAVLMFAIGSLAPSPSLTQQLRIGYIEEAYFEQRGAMARA
                     VGGLPLPPRAVARALAEANLPPTSWPF"
BASE COUNT         1272 a         1830 c         2042 g         1129 t
ORIGIN      
        1 ctcggcatag acgatgtcgc agtcgaagtt cgtggtcggg ccgcaggtcc cgtaagaggg
       61 attggccgtc tggtccttgc cgtacgagat gatcgcgtac gtctggttgc cgactcgata
      121 ctcccaaccc gtgttccagc cgtctttcgg cggcaggacc ttgatgtagg tcggcgccag
      181 catccctgac atctgcgagg gcgacgacga tgtcgatggg atactgcatg ccccagcggc
      241 gctgtacttg tttacgtcgg ttgcgcgcgc ctcccacgcg gtcgcaatcg tacgcatgtc
      301 ggccatggtt cgtttctgct tcgaccgcgc cattgccgtg agcaaattcg gaatagcgat
      361 cgctgcgaga atgccgatga tcgcgacaac gatcaacagc tcgatgagag tgaaaccctt
      421 ctgcttcttt ctaatcattc ggacccctcc tatatgagga ccgtcatcgc aagcgaggtg
      481 ccgagcatca cgatcgcgaa gtctcttttg aatgagtgat ttacgggcga ttcatatgga
      541 tgcccctgca tttgttgacg aaataggcgt gggcgccgtg acaaattgtg tcagggccgt
      601 gacaaaccct cggattcctg cccgaaagcg tatcgctgcg taatgttccg catcaaattc
      661 acgccgctgt cgaaccaatc ggctcgcacc cgttcgtcga tgccgtggat tccctgcgtc
      721 tgataccagt cggtgggaaa gggcatgagc ccgtaggcat cgacgccctt tgcgcggaga
      781 tacttcgagt cgtttgtcgt cgccaccagg atctcactcc cggttcgcgt gccgggatac
      841 tgtttcgatg cttcggatgc gatgagagcg aacatcggcg tgtggcgcga actgatggga
      901 gccggaccgc tcttccgaat cacctgcgcg atcgtcaccc catatcgcga ggtgacaccg
      961 cgcagccatt cgatgcggga gtccgggttt tcgtccggca ggttgtagag attgacgtac
     1021 atggagacgc ctgacccacc atccggttcg atcgtggccg gatagacaat gttttgcgta
     1081 agctcgcggt aacccgttgg gaggagccag aacttgcctt cagcgatggt gcggttgatg
     1141 tcatccagat actgcccctg ctcgatccgc agcggcgcga ggtcgtgcat gaactctttc
     1201 gcctcgggca ggactcgctc cggatcggct ggcgtcatga atggctcgag cgcgatccgc
     1261 aaccgctgca tcgattggcg gtccggcgca tgcaactgca ccttgatcat catcttggtg
     1321 ccggtctcga tgccgaagta ggtgatgtgc tcccgcgtcg tctccgtaat gcccccctcg
     1381 ttgagcgcgt agcgaatgcc cgcgaagaca tcagggcggt gcgcgagcaa ccaggccacg
     1441 cccatcccgc cgccggtctc ctcgtcggcg gtggccagaa atacgatgtc gtgagccggg
     1501 gtgcgatgcg cgcgcgcgag atcgatgaac gccaggagct cgcagacggc gatgcctttc
     1561 atgtccagcg ttccgcggcc atagagctga ttgagtcgga actccgctcc gaacggcgga
     1621 cgagtccagc cctccttatc cgcgggcacg acgtcgatgt gattgagaag gagaagtccg
     1681 gactcgtgtt gtttacccga caaccgtgcg tatacgttcg cacggccggg cgccgactcg
     1741 atgatctcgg cggggattcc attcttcgcc agagttgcgg ccagaaaccg cgcgccggcg
     1801 agctccttac cgggagggtt cgatgtgtcg atgcggacgt actgctggag gagggcgatc
     1861 gccggcgtga tcgttagtgg cttcgggatc cataactgag agttgatgtc ctcgtttgtc
     1921 tgcttgttgt agatgagcag gccgccgata aaggcgagcc cggcggccac tgagacgagc
     1981 agccacttcc agcgacgcat cgactcagcc caccagaccg tcgtcgtccg tcgaaccttc
     2041 tacaccggcc gtttcgaccg ctgccggctc gacgtgggtt ccatcggcgt ccgcgacgac
     2101 atgaatccgt tcttcgtagg cgggcagcgc ggcagcggta tcgcgaggct tgcggtagac
     2161 gatgaggatg ccgatcagga aggcgccggt aatcagggag atcagcatcc ccacgcgcac
     2221 gagctcattg cggtacacca tctcgacgtg gtggtgtccc ggtgtgagga tgattccctg
     2281 gtagccgatg ttcgtcgtga tcgccggtac gcgcgatccg tcgacgcgaa tggtccagta
     2341 cttgtgcggc gtcacactca tcaccagaaa gccctgcccc ccacactcgg catcgaggtt
     2401 gacggtgttc gcggtctcga cgacatggct gaccactccg gccgcgggga cgaacgaagg
     2461 acgggggatg aatgccacca gcgggctgaa ggtccaggtg ctcaactgtt tgacgaagtc
     2521 ctcccgatcg cggatggtga tgatctggtt ggaaaaatag tagcgcggat agtgaggccc
     2581 ttcctcgaag ccgatcggaa gcgattcctt gaagttgccg tggttccgtt tcttctcgcg
     2641 ctcgaagtcc ctgaacgttg cgcggtacca ggcgttcgac atggccatga atggcttcca
     2701 ccacgcggcg cggcccgagc gtttgacatc ccatacggag tcgaccagat cgacagtcgg
     2761 caataacgac gtcttgtcgt agtcgcgctc cagaactgtt tgcacgcgcg cggcggcagg
     2821 tgtcatcgga aaaaggccgt tgcgcacgac ccagtagacc gcctctcccg tggagaaata
     2881 ctgttgcgcc ggtttctcgg ttccgtacca atcggcctcg tgaaagacgc ggtagtcttg
     2941 tcgattcggc gggagcgctg ccgcggctcg cggcagatca aagaatcgcc gcggctcccg
     3001 gggattgagc tcgtacgtga cgaaggcgag atcgatcgtc acgaagagag cggctgcggc
     3061 aagccatacc cggcggcgcc gcttgcgaac catgagtagc agcgcgatga gaatgaggcc
     3121 gcgcaacgtg gcgaagaccc agtcggtgcg cgagagcagg agcggatagc taagaccggc
     3181 accggcagtc tgagcccatg cttttctgat cgaggagagg tcgttgatct tgcccaggag
     3241 gatgaataga cggggataga accgtgtgaa cgcagagacg gcgattccgg cggcgaccag
     3301 cgtcgatgcc gctgcaaagc cgagggctcc gctacggaca tcgtcatcgc catccagcat
     3361 ccgatcgagc atctgggagg caaagaggac taacgcgaat accgccatga ggatgaactt
     3421 ctcgggatag cggatggacg tggcgatgcc ggcgtcatag agccagtggt agagcggagt
     3481 gtggccgccg acggccagga gcgacgaaaa gaaaacgatg atgaggacga gccggccacc
     3541 gcgaggccgt gcgaaggctc ctccgatggc gagcgcaatg acggcgatgc cgacgtagat
     3601 gctgaacaga aacggcgagg ccatccccgg gtacaggccg ccggcccagt agtacatccg
     3661 attctcgatc gagatgtggc cgaggatgtt cgggtagatg agctccgcaa acttcgccca
     3721 gggcatgctc caggcgctga cgaggtcgaa gtcgaatgga cgcgagcgcg ccgagtcacg
     3781 gacgtgatcg atggccggaa gaatctgtgc cgcgccgagc agaaaaccgg tcgccgaaat
     3841 gaaggcaatc cataccacgc gcgtgatgag cttcgaagcg cgcggccggc tgtaccagcc
     3901 tcgatagagc ccgtacatcc cgatcaggag cccggtctgc atgaccgtgg tcggctccgc
     3961 gaccaggaac tggatgccga gaaagagcgc tgccgccgcg aagtcgcgca tgttccgtcg
     4021 cagaaggaat cgccggacgt aaagacacgt gaggggaatc cacgcagcgc agaaaagaat
     4081 cggcaggaga ttgatgtagg agagaaagag accgccgaga ccgaacgata aggcgcccat
     4141 ccacgccgcc ggcgggcgga gatccatcga gcgcaacaag gcatacatgc cgaacagcgc
     4201 gatgtagatg tgaatgagga ccaggagccg gtatccgaga tcgtagctcg gcaggaggat
     4261 cagccaggtg agcggataga agacttcatg ctccggattc gccgcgagcg gctggccggc
     4321 ggagaagtag cggttccagt acggaaactc accatgctgc gcgacgtccc gaaggatctg
     4381 cttggcgggg tagtagtagc gcgtcatgtc gcgcatgtag agctggttga tgccggccag
     4441 gacatcggcg aagcaaatcg tcgcgatcag aaagagaagg ccgagcgcga gcagatcggt
     4501 gcgccggctg accggtatcg gaggcttgtc actcacggct cgcgattgta gtcgagtcgg
     4561 aaggtccgcc atttgcgctg tgttattctc gcgcgcccat gccgcaccac gagaagaaag
     4621 ttgtcgccgt tctgccggcc tacaacgcgg aaaaaacgtt gcaggcgacg ttcgacgaca
     4681 tcccggctga ctgggtcgat gagatcctcc tcgtcgatga ccgttcacgc gaccggacgg
     4741 tcgagctggc gcgtcaactg ccgatgcgcg tggttgttca tgaacacaac cgcggttacg
     4801 gcggcaacca gaagacctgc taccgcacgg ccatggacga gatgggtggc gagatcatgg
     4861 tcatggtcca tcccgaccat cagtacgatc cgaagatcat tccgcaactc gtgcagccgc
     4921 tgctgcgcgg cgattgcgaa gcggtgttcg gatcgcgcat gctcggcggc cgtccgatgg
     4981 aagggggcat gccgaagtgg aagtacctgg caaacctttt tctgacgatg gtcgagaacg
     5041 cgaccttcta catcttcctc tcggagtacc actctggatt ccgcgcgtac acgaagcgct
     5101 atctcgatac tgtgaatttt gaagcgaact ccgacaactt cgtcttcgat accgagatca
     5161 tcgcgcaggg cgttgccaag gggatgcgca tccgcgaggt accgatctcg acgcgctatt
     5221 tcgacgaggc ctcgcagatc ggtttttggc gctcggtgcg ctacggcctc gagatcctca
     5281 agacgatggt cctctacaag ttgcacaaga agcatctcta ctcgcaccgg atctttcgcg
     5341 acccggtgcc gcgcggtact gtcagaacgg ccacgacgta ggcggcaaat tcgcttccgc
     5401 cagggcgcgc gcgacggctc gcggcggcag cggaagcccc ccgacagcgc gcgccatcgc
     5461 cccgcgctgt tcgaagtagg cctcctcgat gtagccgatc cgcagttgct gcgtgagtga
     5521 cggcgaaggc gcgagcgaac cgatggcgaa catcaggact gcgccgaggg cggcgagcag
     5581 agcattccgc ctcgtcgcga tgatgatcgc tgccgcgacg atcgcgagcg gaacgatgat
     5641 cgcgagcggc cgccacacga gatggaggag gttcggggcg acgaggccgt cgcgaagcaa
     5701 cggcagagcg agcgtggacc acggcatgat gaaactgcgg tccggaaacg ggaaggtcag
     5761 tgtgatcgga acgaccgctg ctgccgatgc gccgagaaga atcgctgcga tgcgtcgtcc
     5821 gcgcgcgaac gccagcggga agagaagaaa cggaatcgcg cctgcgatgt agcgcggccc
     5881 gacgttccag ccgccatgcc agttcggata accggagtag atggcgatga gtgccgccgg
     5941 ggtgagaagc aacgtcacga acgcggcgcg gggcaacgct cgttttgccg cgggaatcgc
     6001 aaggagtgct gcggcgatga tcggagcgaa gataagaagc ccgcgcgctg gatcgaacag
     6061 aagccgcatc aggatttcga ggctgggcgc ctgcagtccg aagaggccgg tatgtgccag
     6121 cgcccggtac tccggcagtt tctcgtacgt gtatggcgac gccagcacgc tgccgaacgc
     6181 gagtttctgg tagagcgcca gcatcaacgc gaacggtgcg ccgcccgcga ccacgcgcgc
     6241 aaggcgtccc cacgagcgct gcgccagaag acc
//
LOCUS       DATLVW010000265         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_381067, whole genome shotgun sequence.
ACCESSION   DATLVW010000265 DATLVW010000000
VERSION     DATLVW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381067"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1005
                     /locus_tag="VNN25_08380"
     CDS             208..1005
                     /locus_tag="VNN25_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HXH91583.1"
                     /translation="MHHFIDEAGDLSLFNKRKQVVLGMEGVSNYFMVGAAFVADPEAL
                     ERQLAALRESILRDPLLNRIPSLDPARRKTAASFHAKDDAPEVRYKVFSLLAGLDIQV
                     FVAIRSKDALVEDGRRLFRRGIRLSQNEIYDDMVSRLLRNRLHLAESNTIIVARRGSR
                     DRKEALTVAIGRAQANFASRWGERNFGPCDIATAYPHESGGLQAADYFLWALQRLYER
                     REDRYFAPLSAHYRIIMDLDDTRRKAYGEWYSDSNPLSLEKLKPVTG"
     gene            1118..1879
                     /locus_tag="VNN25_08385"
     CDS             1118..1879
                     /locus_tag="VNN25_08385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXH91584.1"
                     /translation="MRLDQAIAARFPEISRRRARELIAARRVLVNERAVSIASREVNA
                     HDRIAVAEDEPEIAIIRETDDWIAADKPAGMPTQPARDRVQRSLEELLRLRFHAIWLV
                     HRLDTPTSGVVLFARSQTAAAELSALFAQGAIRKTYVAIVEGVMTDEQVVDTAVQGKE
                     ARTTFRPLRNDGASTLVEAVIDTGRTHQIRIHAASINHPVAGDRRYGSGARAKRLMLH
                     AWKLEHPRFGVLEAPLPTSFSLSPALPAFAPPIPR"
     gene            complement(1821..>2937)
                     /gene="glmU"
                     /locus_tag="VNN25_08390"
     CDS             complement(1821..>2937)
                     /gene="glmU"
                     /locus_tag="VNN25_08390"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXH91585.1"
                     /translation="GRKVLILSGDVPLTHPQTLQQLIDEHEQSQNALTLLTMKPADPG
                     MYGRVVRDAGGAVIRIVEAKDATDAEKQIGEVNAGIYVFDSAHLFGNLRNLSTDNGQG
                     EYYLTDLLAMLRNAGHRVGAVVASDPIEALGVNSRAELATIEGEIQRRVVTKLMREGV
                     TFRNPATVVIDSTVAIGNDSVVYPYVTIEGETTIGSRCVIEPGVHLTNVTVGDDVYLK
                     TGTVAEDAVIESEASIGPYAHLRPGTRLGRHVKIGNFVETKKAIFGEGAKASHLSYIG
                     DAEVGEDVNIGAGTITCNYDGVNKHKTVLEDGVFIGSDTQLVAPVRVGKGAYVGAGST
                     ITKDVPAGALALSRTPQRVIAGWAARKREERAKEKTK"
BASE COUNT          566 a          895 c          881 g          595 t
ORIGIN      
        1 ggtccgccgc gccatcggca tcggcgtacc ggagctgacg gtgatcgaag gcggcgaacc
       61 cgcaatcgct gccgctcctc cctccaagcc gacgagagtg gccgcgaagt aggtctgcaa
      121 aatcgattca ggagcgaaag gcacagccgc aggctgtgcc tttcgtgttt cttgagaatg
      181 acgacggcga agccgagagt gcctaggatg caccacttca ttgatgaagc tggtgatctg
      241 tcactcttca acaaacggaa acaggttgta ctcggaatgg aaggcgtctc caactacttc
      301 atggtcggcg cagcgtttgt cgctgaccct gaggccctcg aacgacaact cgcggccctt
      361 cgcgagagca ttcttcgaga tccactactg aatcgcattc cgtcgctcga tcctgcgcga
      421 agaaaaacgg cagcatcgtt tcacgcaaaa gatgatgcgc ccgaggtgcg atacaaggtc
      481 ttcagtctgc ttgcggggct ggatattcaa gtttttgtcg cgatccgctc gaaagacgct
      541 ctcgtcgaag atgggcgccg gctctttcgc cgtggtatcc gcttgtcgca gaacgaaatt
      601 tatgacgaca tggtgtcgcg cctcttgcgg aacagactgc atctggcgga atcaaacacg
      661 atcatcgtcg ccaggcgggg ctcacgagat cgcaaagagg cattaacggt agcgatcggc
      721 cgtgcgcagg caaacttcgc atccaggtgg ggggagcgca atttcgggcc ctgcgatata
      781 gccaccgcct atccgcatga gtctggcggc ttgcaagctg ccgactattt tctctgggcc
      841 ttgcaacgtc tctacgagcg gcgagaggat cgttacttcg ctccgctctc ggctcactat
      901 cggatcatca tggatctgga tgacacgcgg cggaaggcct atggcgaatg gtacagtgac
      961 tcgaatccgc tatcgctgga aaaactaaaa cccgtaacag gctaggttcg atcccagtgg
     1021 gaccgaacac ccggcatgga gccgactttc gcctgttccc gggtcagtcc aatgtaacgg
     1081 atacttccgg tcaaaacaat cctcaaatca aggaatcatg cgactcgacc aggccatcgc
     1141 ggcgcgtttt ccggagatct cgcgtcgccg cgcgcgcgag ttgatcgcgg cgcggcgcgt
     1201 gttggtcaac gagcgggcgg tgtcgatcgc gtctcgcgaa gtgaacgcgc acgaccggat
     1261 tgctgtggcc gaggatgagc cggagatcgc gatcattcgg gaaacggacg actggatcgc
     1321 tgccgacaag ccggccggga tgccgacgca gccggcgcgc gaccgggtac agcgttcgct
     1381 cgaggaattg cttcggcttc gtttccacgc catctggctc gtgcatcggc tggatacacc
     1441 gaccagcggc gtagtcctct tcgcacggtc tcaaactgcc gctgccgagt tgtcagcgct
     1501 cttcgcacag ggtgcgatcc gcaaaaccta tgtcgcgatc gtcgaaggtg tgatgacgga
     1561 cgagcaggtc gtcgacacgg cggttcaggg gaaggaagcg cgaaccacgt tccggccgct
     1621 gcgcaatgat ggcgcctcga cactcgtgga ggcggtcatc gataccggcc gcacgcacca
     1681 gattcgcatt cacgcggcgt cgattaatca tcctgttgcc ggcgatcggc gctacggctc
     1741 gggagcgcgc gcgaagcggc tgatgcttca cgcctggaag ctcgagcacc cgcgtttcgg
     1801 cgttctcgaa gcgccgctac ctacttcgtt ttctctttcg cccgctcttc ccgctttcgc
     1861 gccgcccatc ccgcgatgac gcgttgcggc gtgcggctga gggccaacgc gccggccggc
     1921 acgtccttcg taatcgttga gcccgcgccg acgtaggccc ctttgcctac gcgtaccggc
     1981 gcgaccagtt gggtgtcgga accgataaaa acgccgtcct cgagcacggt cttatgcttg
     2041 ttcacgccgt catagttgca ggtaatcgtt cccgcgccga tgttcacatc ctcgccgact
     2101 tcggcatctc caatgtagga aaggtgagag gcttttgccc cttcgccaaa gatcgctttc
     2161 ttggtctcga cgaagttgcc gatcttgacg tgacgtccga gccgcgtacc gggccggaga
     2221 tgcgcatacg gacctatcga ggcctcgctt tcgatgacgg catcctcggc cacggtcccg
     2281 gttttgaggt agacatcgtc gccgacggta acgttggtga ggtgcacccc aggctcgatc
     2341 acacagcgcg agccgatcgt ggtctcgcct tcgattgtca cgtacggata gacgacggag
     2401 tcgttgccaa tggcaacggt cgaatcgatg acgacggtgg ccggattgcg gaacgtgacg
     2461 ccctcgcgca tcagcttcgt gaccacacgg cgctgaatct ccccttcgat cgttgccagc
     2521 tcggcacgcg agttgacgcc gagcgcttcg atcggatcgg atgcgacaac agccccaacg
     2581 cggtgaccgg cgttgcgcag catggccaga agatccgtga ggtagtactc cccctgccca
     2641 ttgtccgtcg acagattcct gagattgccg aacagatggg cgctgtcgaa aacgtagata
     2701 cccgcattca cttcgccgat ctgcttctcc gcgtcagtcg catcctttgc ttcgacgatc
     2761 cggatgaccg cgccaccagc gtcccgcacg acgcgtccgt acatgcccgg atccgcgggt
     2821 ttcatcgtca ggagtgtcag cgcgttttgc gactgctcat gctcatcgat caactgttga
     2881 agagtctgcg gatgcgtgag tggaacatcg ccggagagga tgagaacctt tcggcca
//
LOCUS       DATLVW010000266         4868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_10604, whole genome shotgun sequence.
ACCESSION   DATLVW010000266 DATLVW010000000
VERSION     DATLVW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10604"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VNN25_08395"
     CDS             complement(<1..733)
                     /locus_tag="VNN25_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH91586.1"
                     /translation="MPLVPELNVADRTCVVGDITIHYVEAGRGPLIVLLHGFPEFWYS
                     WRNQIPALAAAGFHVVAVDLRGYNESSKPRAIESYRLPELAKDIAGLITQIGKGPAVV
                     AGHDWGGAVAWFMAMLYPEQVKRLIILNSPHPVAYLREMRRSARQKWRAKYQLLFHPP
                     WLAEFVLRRFRYALLRRSLSRLGCFTPEEIERYVEAWSKPGALTGMANYYRATRLRKG
                     MRGLVRRIDIPTLMIWGDLDPFFTPE"
     gene            complement(1089..1463)
                     /locus_tag="VNN25_08400"
     CDS             complement(1089..1463)
                     /locus_tag="VNN25_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91587.1"
                     /translation="MDTPMENTGFGSAPTPTSSGGTGSMASASSGYCPTCGQSRSATG
                     TGALEQFLGRLGITEEMITNLKSSIQNVDIEEYLTTARDYLKGGSEKATTYAKENPGK
                     VAAGVAVLAVGTGLLISALNRD"
     gene            complement(1468..1917)
                     /locus_tag="VNN25_08405"
     CDS             complement(1468..1917)
                     /locus_tag="VNN25_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91588.1"
                     /translation="MPRKQGKSAVKRAGGAVARTARKLATKLDPRKHAKVAAAPAAKK
                     ARAAKPKTVPTKTTPAPSRPVRRESDVPMDLLAQTYAPRQTSIKTSFRSDGADHERDQ
                     ELPPGPGERFENEDHFTNKSGDSRIGTHGRTYEPVEQRAATANAARR"
     gene            complement(2349..3581)
                     /locus_tag="VNN25_08410"
     CDS             complement(2349..3581)
                     /locus_tag="VNN25_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXH91589.1"
                     /translation="MKRRLVLLTAVAIACSPESSRLPAAINSAHDPMLQHHEIRIDQL
                     PKPFATPSAGNPPHVESQPSNATLHLLPGFHISLFADKLDNPRAMLQAPNGDVIVSEP
                     GAGKIIILRDANHDGIAEQRFTFATGLDNPYGLAFHDRFLYVGSESALLRFPYAPGQT
                     SANGKPQVIGPLPSGGHSTRGVLFNRAGTKMYVSIGSNSNVSAGEGPERASIIELNPD
                     GSGRHVFAGGLRNPVGMAWEPVTGALWTAVNERDGLGDELVPDYATDVKAGAFYGWPY
                     AYLGAHEDPRRAGERPDLVKRTIPPAVPIQSHSAPLGIAFYQGSMFPPEYRGRAFVAL
                     HGSWNREPRTGYKVISIPFKNGKPTGGYDDFVAGWMPDETSRTVWGRPVGLLVLADGS
                     LLISDDGGEKIWRVTYGK"
     gene            3864..>4868
                     /locus_tag="VNN25_08415"
     CDS             3864..>4868
                     /locus_tag="VNN25_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXH91590.1"
                     /translation="MTTAPDTVAPWQFDGVVRLTRVGSSFVVFTIVVGFAAINTGNNA
                     LYIGLSFMLGCLLLSGIASKGGLKHLVVELEGVDEAWAMRTAHGRLRVINRSRIWNVR
                     DLIITSNDLEAPLAVPLIGRRAEVVLQARFRFRRRGLVHLSRIDLYTRYPFGFFLKKR
                     RPRLDAEIVVFPRLLDEESTRERFRTIEGEQYSSNRIGAGTDIHSFRDYSRGDSLRRV
                     HWKKSASVGRWIIKQTELEAGRAVHVVVDPYRPKDVSEEAFEEMISDAATFLNESLRR
                     GLHTTLSIRNLTIRSDRDGAAATFRALALLEATHEPVAQTVDRDSVIFGAGVVGRESG
                     V"
BASE COUNT          884 a         1413 c         1625 g          946 t
ORIGIN      
        1 tctcgggggt gaagaaggga tcgaggtcgc cccagatcat gagggtgggg atgtcgatgc
       61 ggcggacgag gccgcgcatg ccctttctca ggcgggtggc gcgatagtag ttggccatgc
      121 cggtgagcgc tcccggtttc gaccaggcct cgacgtagcg ctcgatctcc tcgggggtga
      181 agcagccgag gcgggagagg gagcggcgga ggagggcgta gcggaagcgg cggaggacga
      241 actcggccag ccacggcgga tgaaagagga gctggtattt ggcgcgccat ttctgccgcg
      301 cggagcggcg catctcgcgc aggtaggcga ccggatgggg tgagttgagg atgatgaggc
      361 gcttgacctg ctcgggatac agcatcgcca tgaaccaggc cactgcgccg ccccaatcgt
      421 gtccggcgac gacagccggc cctttgccga tctgcgtgat caagccggcg atgtctttgg
      481 ccagttcggg cagtcggtac gattcaatcg cgcgcggctt tgacgattcg ttgtatccgc
      541 ggagatcgac ggccacgacg tgaaagcccg ccgcagcgag cgccggaatc tgattgcgcc
      601 acgagtacca gaactcgggg aagccgtgga gcagaacgat gagcggcccg cggcccgctt
      661 cgacgtaatg aatggtgatg tcgccaacaa cgcaggtgcg gtcggcgacg ttcaactcag
      721 ggaccagcgg cattggggca ttgtacgaga gagccgctgt ctcctcagcc aagctctgat
      781 tcttccgacg agcaaacagc gacactcgac catcgagcac cttctctcaa cgatgctcga
      841 acatcgagcg ccttctcccc cacgcttttc agtgggggag aaggtggccg aaggccggat
      901 gagggggtgt ttggctagtg cttaagacgt ccgcgaaggt cgattggaac ccgcttcgat
      961 gatcaggaat cgtgagttgg tcgcccccct catccggctt cgccaccttc tccccccaca
     1021 aaaaacgtgg gggagaaggc gctcgattga gaagtgttgg cgagagggtt ggggtggctt
     1081 tgaccgactt aatcccgatt caatgcgctg atcagcagcc ccgttcctac cgcgagcact
     1141 gccacgcctg cggcgacctt gccggggttt tctttcgcat aggtcgttgc tttttcgctg
     1201 ccgcctttga ggtagtcgcg ggcggtggtg aggtactcct cgatgtcgac gttctggatc
     1261 gacgacttga ggttggtgat catctcctcg gtgatgccga gacgcccgag aaactgctcg
     1321 agagcgccgg tccctgtggc agagcgggat tggccgcagg tggggcagta gccgcttgat
     1381 gccgaagcca ttgaaccggt cccgccgctc gaggttgggg ttggggcgct tccgaagcct
     1441 gtgttttcca tcggtgtatc catgtcgtta tctccttgct gcgtttgcgg tggctgcgcg
     1501 ttgttcgacc ggctcgtagg tgcggccatg cgtgccgatc cgtgagtcgc cggacttgtt
     1561 ggtgaagtgg tcttcgttct cgaagcgctc gccggggccg ggcggcaatt cctgatcacg
     1621 ctcgtgatcg gctccatcgc ttcggaacga cgtcttgatg ctggtctgcc tcggtgcgta
     1681 ggtctgcgcg agaaggtcca tcgggacatc ggactctcgt cgtacaggcc tggacggtgc
     1741 cggagtggtc ttcgtcggca ctgtctttgg ttttgctgct cttgctttct tcgctgccgg
     1801 agcggcggca acctttgcat gcttccgagg atcaagcttc gtcgccagtt tccgggcagt
     1861 gcgtgcgacg gcgcctccgg cccgtttgac agccgatttc ccctgctttc gtggcatctg
     1921 tcttcctttc gtcgtgtggt gtgtgacgaa ggtggtcagt gcaagaggga agccgaagga
     1981 gtgcgggcgt ctcgctcgcg gcgccgaggc gtcctcgcct cggcgtgtgt tgcgcgaagc
     2041 cggatcccgg gaccaatttc gagcaggtca aaaccgtacc gaaatgggcg cggaaccaat
     2101 ttcaagaagg tcaaaaccca ccgaaatggg tccgggacca acttgaagaa ggtcgaaacc
     2161 ctaccgaaat gggctcggga ccaatttcag gaaggtcaaa accctaccga aataggtccg
     2221 ggaccaattt caggaaggtc aaaaccctac cgaaattggt ccgccaccaa tttcaggaag
     2281 gtaaaaacct taccgaaatg ggtctgggac aaatttcagg aagataaaag tcctagcgct
     2341 gtcgccccct acttcccgta cgtcacccgc cagatctttt cgccgccgtc gtcggagatg
     2401 agcagggatc cgtcggcaag aacgagaagg cctaccggac ggccccacac ggtccggctc
     2461 gtttcatccg gcatccagcc agcgacgaaa tcgtcgtatc cgcctgttgg cttcccattc
     2521 ttgaacggga tgctgatgac tttgtagccg gttcgcggct cgcgattcca tgaaccatga
     2581 agcgcgacga aggcgcgccc gcgatactcg ggaggaaaca tcgaaccttg atagaaggcg
     2641 atgccgaggg gcgcggaatg ggactgaatg ggcacggcag gaggaatcgt cctcttgacg
     2701 aggtcaggcc gttcgccggc gcggcgcgga tcttcgtgcg cgccgaggta ggcgtagggc
     2761 cagccataga aggcgccggc tttgacgtcc gtggcgtagt cgggaaccag ctcatcgccg
     2821 agtccgtcgc gctcgttgac cgcagtccac aacgcgccgg tcaccggctc ccacgccatc
     2881 ccgacaggat tgcgcaggcc gcccgcgaac acgtgtctgc cgcttccatc cggattgagc
     2941 tcgatgatgg aggcgcgctc cggtccttcg ccggcgctga cgttcgagtt ggagccgatc
     3001 gacacataca tcttcgtccc cgcccggttg aacagcactc ctcgggtgga atgaccgccg
     3061 gacgggagcg gtccgatgac ctgtggcttt ccattggcgc tcgtctgccc cggagcgtaa
     3121 ggaaagcgga gaagagcgct ctcgctgccg acgtacagga agcgatcgtg aaaggccagg
     3181 ccgtaagggt tgtcgagacc ggtcgcaaag gtgaagcgtt gttccgcgat gccgtcgtga
     3241 ttcgcgtcgc gaaggatgat gatcttcccc gctcccggct cggagacgat cacatcgccg
     3301 ttgggcgcct gtagcatcgc gcgcggattg tcgagcttgt ccgcgaagag cgagatgtga
     3361 aagccgggca ggaggtgaag cgtggcgttg gaaggttgcg attcgacgtg cggagggttg
     3421 ccggccgagg gagtcgcgaa gggtttcggc aattgatcga tgcggatctc gtgatgctgc
     3481 agcatcggat cgtgcgccga gttgatagct gcgggcaggc ggctcgattc aggagagcat
     3541 gcgatggcga cggcggtgag aagaacaagg cggcgcttca tgcgaaaagt atgcgtgccg
     3601 cgaggtgatg cgttggaaaa catttgatca cggggagtgg ggagtggggt gtggggggtg
     3661 gagaacggcg cgcctccagg aaaaccggtc acgcgaagac gcgaagacgg gaaggatgcg
     3721 acgggcgaag acgtggatgt gcggagcgac cgtgattcac ccgttctccg acccccgacc
     3781 ccccgcaatc aatccaaccc caccccccac cccccacacc ctacccccgg aatcccaaac
     3841 cgcacccacg cattgaaagc tccgtgacca ctgcgcccga caccgtggct ccctggcaat
     3901 tcgatggcgt cgtccggctc acgcgcgtcg gctcgtcgtt tgtcgttttc accatcgtcg
     3961 tcggatttgc cgcgatcaac accggcaaca acgcgctcta catcggtctc tccttcatgc
     4021 tcggctgcct gctgctgtcg ggcatcgcat cgaagggggg actcaaacat ctcgtcgtcg
     4081 agctggaagg tgtcgatgag gcgtgggcga tgcggaccgc gcacgggcgt cttcgcgtga
     4141 tcaaccggtc gcgcatctgg aacgttcgcg atctgatcat cacttcgaac gacctggaag
     4201 cgccgctggc ggttccgttg atcgggcggc gcgccgaggt cgtgctgcag gcgcggtttc
     4261 gcttccgacg gcgcggtctg gtgcacctct cgcgcatcga tctgtacacc cgctatccgt
     4321 tcggattctt tctgaagaag cgacgcccgc ggttggatgc tgagatcgtc gtttttccgc
     4381 gcctgctcga tgaagaatca acccgggagc gctttcgcac gatcgaggga gagcagtatt
     4441 cgtcgaaccg catcggagcg ggaacggaca ttcattcgtt tcgtgactac agccgagggg
     4501 attcgcttcg ccgcgtgcac tggaagaaat cggcatcggt ggggcgctgg atcatcaagc
     4561 agaccgagct cgaggccggc cgcgcggtgc atgtcgtcgt cgatccgtac cggccgaagg
     4621 atgtgagcga ggaagcgttc gaggagatga tcagcgacgc ggccacgttc ctcaatgagt
     4681 ccctacgtcg cggacttcat acgacgctgt cgatccgcaa cctgacgatt cgctccgacc
     4741 gcgacggcgc cgccgcaacg ttccgtgctc tggcgctgct cgaggcgacg cacgagccgg
     4801 tggcgcagac cgtggatcgg gacagcgtga tctttggcgc gggggtcgtg ggtcgggagt
     4861 cgggggtc
//
LOCUS       DATLVW010000267         5098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_54818, whole genome shotgun sequence.
ACCESSION   DATLVW010000267 DATLVW010000000
VERSION     DATLVW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54818"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2665)
                     /locus_tag="VNN25_08420"
     CDS             complement(<1..2665)
                     /locus_tag="VNN25_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91591.1"
                     /translation="MSEPRFSRLALIIATVSLFLSIPAMAATTLSIAPPPVSWNIIGL
                     DSNTPATGPHLFPVGTRVCSSAATTNVSVSFFFDTANANVNLRAGSLSTVVIPAIGAG
                     QCKDAYFEVDVTQTAAAFDTARRYHITATDGSGSVTTVTPRELYVEHLISQNRNSVTD
                     VKFGPVGCGVANTCTSVAPGGAMNLIVGSSYDIQLLGGTATQGYNQFEAFINFPNTIF
                     QIQSVATTYSADNSPYVPGPAPTVNDKLYADACLWQNDPNSPAYRSCVGGDFKAGGSN
                     VVTTYSIKIVSGGGTSQTLGSLLYDFSGSSYHYNSDYGVGARIANIIDPTAANISKAF
                     TPNPTSLNGISALKITLTNPNPVAVSGYNFVDNLPANLVVATPPAATTSGCGAPTLTA
                     VAGSSSITFSNGTVAAGSPGTCVISVNVTPTATGSLVNTTNHLFIDTDDTGHSGTDTL
                     TVNSAPPPGTGLCGVTMASWTVPDGTVANPPDLAGGLPTTKAANVVTATLAANVPADT
                     AIVTTGGHGDTTAWRTNGYKNDGNFIQFTIDTTNYTNVHFSFWVANPSPANGPTAFNL
                     TINNGAGFGAPIVLAVPPIAFTNYNIDATGLTNVAGNTIFRLTATGANNDAGGASFNY
                     DDMLFTGCTAAVKATITKTFVPNPIAVGAVSTLTFTLTNANAAPLTGATFTDSLPTGV
                     QVAATPSASTTCGGGPTWAPAAAATTLTFGSPTGGTIPANGTCTVSVNVTATTSGPHS
                     NTSGFLFTTEGGTNTSSVASASLTAVLPPSIAKEFAPSPILAGGISRLTFTITNPNPN
                     DAISGVNFGDTFPTSPGAMVVAGTPNPSTTGCGAPTFSPVAGAGSITFSGGAIAPGGT
                     CTVALDITAPATGTYNNTSSPVSHIINAQTVN"
     gene            3036..>5098
                     /locus_tag="VNN25_08425"
     CDS             3036..>5098
                     /locus_tag="VNN25_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011636493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH91592.1"
                     /translation="MSGLEKVVIPPVTDRRARKSKAASDAAVTEIRRKKALLKTGALQ
                     NAILNSANFSSIATDEKGVIQLFNVGAERMLGYAAADVVDQITPADISDPQEVIARAA
                     ALSLELGTTITPGFNALAFKASRGIEDIYELTYIRKDGSRFPAIVSVTALRDVQNAII
                     GYLLIGTDNTARKQAEEALIKAGALQNAIFNSANFSSIATDAKGVIQIFNVGAERMLG
                     YAAAEVMDKITPADISDPEEVIARAAALSRELETPITPGFEALVFKASRGIEDIYELT
                     YIRKDGSRFPAIVSVTALRDVQNAIIGYLLIGTDNTARKQVEAEQKRLDQRLRDQQFY
                     TRSLIESNIDALMTTDPSGIITDVNKQMEALTGCTRDELIGAPFKNYFTDPERAEAGI
                     KLVLSETKVTNYELTARARDGKETDVSYNATTFYDRDRRLQGVFAAARDVTERKRLDE
                     VLHDKNIELESARVVAEKANLAKSDFLSSMSHELRSPLNAILGFAQLMESDSPPPTQL
                     QTDSISQILNAGWYLLDLINEILDLAMIESGKLSMSLEPVSLTEVMLECQAMIEPLAQ
                     RRGIHMTFPTLDVPHSVRGDRTRIKQVLINLLTNAIKYNQVGGSVVVGCCISPNAGHV
                     RMTVTDTGAGLTPEKLSQLFQPFNRLGQEGNTEEGTGIGLVVSKRLVELMNGVIGVES
                     TVGTG"
BASE COUNT          997 a         1516 c         1654 g          931 t
ORIGIN      
        1 cgttcaccgt ctgcgcgttg atgatgtgcg agacggggct gctggtgttg ttgtacgtgc
       61 cggtggccgg cgcggtgatg tcgagggcga cggtacatgt cccgcctggc gcaatcgctc
      121 cgccgctgaa cgtgatcgag ccggcgccgg caacgggact gaacgtcggc gcgccacaac
      181 cggtggtcga tggattcgga gtcccggcta cgaccattgc gccgggtgaa gtcggaaacg
      241 tatcgccgaa gttgacgccg ctgatggcat cgttcggatt cggattcgtg attgtgaagg
      301 tgaggcgtga gatgccaccg gcgagaatcg gcgatggcgc aaactctttg gcgatcgatg
      361 gcggaagcac ggccgtcagc gacgcagacg cgacgctcga cgtatttgtc cctccctcgg
      421 tcgtgaacag gaacccgctg gtgttcgagt gcggacccga cgtcgtggcg gttacgttga
      481 cgctgaccgt gcaggtgccg ttggcgggga tggttccacc ggtaggtgat ccgaacgtca
      541 gcgtcgttgc cgcggccgca ggcgcccagg tcggtccgcc gccgcaggtc gtgctcgcgg
      601 atggcgtggc cgccacttga acaccggtag gcagcgagtc agtgaacgtg gcgcccgtca
      661 gaggcgctgc gttcgcgttg gtgagcgtga acgtcagcgt cgacaccgca ccgacggcga
      721 tcgggttggg cacgaatgtc ttcgtgatcg tcgccttcac cgccgcggta cagccggtga
      781 agagcatgtc gtcgtagttg aatgaggcgc cgccggcgtc gttgttggcg ccggtagccg
      841 taagcctgaa gatcgtattt ccggccacgt tcgtcaggcc ggttgcatcg atgttgtagt
      901 tcgtaaacgc aatgggcgga acagccagca cgatcggcgc tccgaaaccg gctccgttgt
      961 tgatggtgag gttgaacgcg gtgggaccat tggccgggct cggattggct acccagaagc
     1021 tgaaatggac gttggtgtag ttcgtcgtat cgatcgtgaa ctgaatgaaa ttgccgtcgt
     1081 tcttgtagcc gttcgtgcgc caggctgtcg tgtcgccgtg cccgccggta gtgacgatcg
     1141 ccgtatccgc ggggacattc gcggcgagcg ttgccgtaac cacattcgcg gctttcgtcg
     1201 tcggcagacc gccggcgaga tcgggaggat tggcgaccgt gccgtcaggc accgtccacg
     1261 atgccatggt cactccgcac agtccagttc ccggaggcgg cgcgctgttg acggtcagcg
     1321 tgtccgtgcc ggagtgtccg gtgtcgtccg tatcgatgaa aagatggttc gtcgtattga
     1381 cgagactccc tgtcgccgtc ggcgtgacgt tgacgctgat cacgcaggtg ccggggctgc
     1441 ccgcggcgac cgttccgttc gagaacgtga tcgagctcga tccggccacg gcggtgagcg
     1501 tgggcgcccc gcagccggat gtcgttgcgg ccggcggcgt ggcaacgacc aggttcgcag
     1561 gaaggttgtc gacgaagttg tagccgctca ctgccaccgg attcggattc gtcagcgtga
     1621 tcttgagagc ggagatgccg ttcagactcg tcggattcgg tgtgaatgct ttgctgatgt
     1681 ttgcggccgt cgggtcgatg atgttggcga tgcgcgcgcc gacgccgtag tcgctgttgt
     1741 agtgatagct gctgcccgag aaatcgtaga gcagcgagcc caatgtctgg ctcgtgccgc
     1801 cgccgctgac gatcttaatg ctgtacgtcg tgacgacgtt gctgccgccg gctttgaaat
     1861 caccgccgac gcaggaacgg taggcgggac tgttcggatc gttctgccag agacaggcgt
     1921 cggcgtagag cttgtcgttg accgtcggcg ccggacccgg aacgtacggc gagttgtccg
     1981 ctgagtacgt ggtggcgacg ctttggatct ggaagatcgt gttgggaaag ttgatgaacg
     2041 cttcgaactg gttgtagccc tgcgtggccg tgccgcccag aagctggatg tcgtagctgc
     2101 tcccgacgat gagattcatc gctccgccag gcgcgacgga ggtgcacgtg ttagctacgc
     2161 cgcaaccgac cggtccgaac ttcacatcgg tgactgagtt gcggttctgc gagatcagat
     2221 gctccacata cagttccctc ggggtcactg tggtgacgga tcccgagcca tcggtggcag
     2281 tgatgtggta gcggcgcgcc gtgtcgaacg cagccgcggt ctgcgtgacg tcgacctcga
     2341 agtacgcgtc cttgcactgg ccggcgccga tcgcgggaat gacgaccgtg ctcagcgaac
     2401 cggcgcgaag gttgacgttc gcattcgcgg tatcgaagaa gaagctgacc gacacgtttg
     2461 tcgtggccgc gctgctgcag acgcgcgtcc ccacgggaaa gaggtgaggt cccgtcgccg
     2521 gagtgttgct gtcaaggccg atgatgttcc aggaaaccgg cggcggcgcg atcgagagag
     2581 tcgtcgcggc catggccggg atcgagagga aaaggctgac ggtcgcgatg atcagggcaa
     2641 ggcgggaaaa acgaggctcg gacatttggg ttccctttgt tgaaaccggt tacgcgaagt
     2701 ccacacagag gcgccgctgg gtcagcgctt caagaggaat gagttgtttg aaaggacctt
     2761 gctggattac cgggcggcag attagtgcat taagggcgtt gagcaaagta aactttttgc
     2821 gagggaggat gtctcgcaca aagcactcgg gcggggacgc ccgagtgccc gcgggcgaga
     2881 cgcctggcga gacgcccgca ctccgacggc acacgggttg cttggctctc tagtgttccc
     2941 ccttccagga gagcgagatc gtacgtctgg tggtgcgacc accgggcgga cgtcactcgt
     3001 ccattctcct gagcgaagag ggcaaggtga gccccatgag tggattggaa aaggtagtga
     3061 ttcctcccgt aaccgaccgg cgcgcgagga agtcgaaagc ggcaagcgac gccgccgtca
     3121 cggagattcg aaggaaaaaa gctctcctga agaccggcgc gctccagaac gccatcctca
     3181 acagcgccaa cttctccagc atcgccaccg atgagaaggg cgtcatccaa ctcttcaacg
     3241 tcggagcgga acggatgctg ggatacgccg ccgccgacgt cgtcgatcag atcacgccgg
     3301 cagacatctc cgatccgcag gaagtgatcg cccgcgccgc cgcgctgagc ctcgagctcg
     3361 gaacgacgat cacacccgga ttcaacgctc tggcgttcaa agcatcacgc ggcatcgaag
     3421 acatttacga gctgacctac atccgcaagg acgggagccg ctttccggcc atcgtctcgg
     3481 tcacggcgct gcgcgacgtg cagaacgcga tcatcggcta cctgctcatc ggcaccgaca
     3541 acacggcccg caaacaggcg gaggaagcgc tcatcaaagc cggcgcgctg cagaacgcaa
     3601 ttttcaacag cgccaacttt tcgagcatcg ccaccgatgc gaaaggcgtc atccagatct
     3661 tcaatgtcgg cgccgaacgc atgctcggat atgcagcagc cgaggtgatg gacaagatca
     3721 cgccggccga catctccgat ccggaagaag tgatcgcccg cgccgcagcg ctcagccgcg
     3781 agctcgagac gccaatcacg ccaggtttcg aggcacttgt cttcaaggca tcgcgtggca
     3841 tcgaagacat ttacgagctg acctacatcc gcaaggacgg gagccgtttt ccggccatcg
     3901 tctcggtcac ggcgctgcgc gacgtgcaga acgcgatcat cggttacctg ctcattggca
     3961 ccgacaacac cgcacgcaaa caggtcgagg cggagcagaa gcggctcgat caacggctgc
     4021 gcgatcagca gttctacacg cgctcgctca tcgagtcgaa catcgacgcc ctgatgacca
     4081 ccgatccttc cggcatcatc accgacgtca acaaacagat ggaggcgctc accggctgca
     4141 cccgcgacga gctgatcggc gcaccgttca agaattactt caccgatccg gagcgcgccg
     4201 aagccggaat caaactcgtt ctgagcgaga cgaaagtcac caactacgag ctcaccgcac
     4261 gcgcaagaga tggcaaggaa acggacgtct cctacaacgc gactacgttc tacgaccgcg
     4321 accggaggct gcaaggtgta ttcgccgccg cgcgcgatgt gaccgagcgc aaacggctgg
     4381 acgaagtgct gcacgacaag aacatcgagc tcgagagcgc ccgcgtcgtc gccgaaaaag
     4441 ccaatctcgc caagtccgac ttcctctcaa gcatgagcca cgagcttcgt tctccgctca
     4501 acgccatcct gggcttcgcg caattgatgg agtccgactc accgccaccg acccaattgc
     4561 agaccgacag catcagccag atcctgaacg ctggctggta cctgctcgac ctgatcaacg
     4621 agatcctcga tctggcgatg atcgagtcgg gaaaactgtc gatgtcgctg gaaccggtct
     4681 cgctgaccga ggtgatgctc gaatgccagg cgatgatcga gccgctggcg cagagacgcg
     4741 gcatccacat gacctttccc accctcgacg tgcctcactc ggtgagaggc gaccggacgc
     4801 gcattaagca ggttctgatc aacctgctca ccaacgcgat caagtacaac caggttggag
     4861 gatccgtggt cgtcggctgc tgcatctcac ccaacgcggg tcacgtgcgg atgaccgtca
     4921 ccgataccgg cgctggactg actcccgaga aactctccca gcttttccag cccttcaacc
     4981 gcctcgggca ggaaggcaac accgaggaag gcacgggcat cggcctcgtc gtcagcaagc
     5041 gcctggtcga gttgatgaat ggcgtcatcg gcgtggaaag tacggtcggc acgggaag
//
LOCUS       DATLVW010000268         6410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_381132, whole genome shotgun sequence.
ACCESSION   DATLVW010000268 DATLVW010000000
VERSION     DATLVW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381132"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="aroB"
                     /locus_tag="VNN25_08430"
     CDS             <1..928
                     /gene="aroB"
                     /locus_tag="VNN25_08430"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXH91593.1"
                     /translation="PLRDRFGERIAASLDADVIEIEEGESHKNLTTVNDVVTQLLARG
                     AKRDSMAVVVGGGMVGDTAGFAASIFLRGIDLVHIPTTLLAQVDSSLGGKVAVNHASG
                     KNLIGSFFPPRAVISDLSTLDSLPRREMLSGLFEALKGGVIGDASLFEMLESPPRIDV
                     DEVVRKAIQVKAEIVSADEREADLRRLLNYGHTIGHGIEAALDYRNLTHGEGVAWGMI
                     GANAIAVQRGLLARNVAGRIDRAIRAWNPAPIPADIDRNAILAAAEHDKKNTGSQRVM
                     VFPRAIGTCEVFADITEAEIAFGVDAILGEVR"
     gene            937..1833
                     /locus_tag="VNN25_08435"
     CDS             937..1833
                     /locus_tag="VNN25_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91594.1"
                     /translation="MRRQPPIWHFAAVYETRLVLNERLVIAVPPVKVEVITGPAYMFR
                     LSNDRAVVPLMGKVGEEVEIPSPPAIDLEAGKRYIIVSTRTNENPAVVEEECRRVVDR
                     MIGIIGLLSSPTLFALPIYVGWVSATPSTPLNTKAMFSKPEQLNRAALEKGVSIARRV
                     LARDPAMRDRFDLVARLFSRTMGSPPTDEAFLWAWTCLEVFPMMGTQKHRHIAPYLAK
                     LTGDDEAALAARLDIRGLYILRSKLVHSGHLGLGDTELYRSLTILRGIVCTVMRGMCG
                     LPYDGELERVVQLREQPTVIPV"
     gene            complement(2025..2207)
                     /locus_tag="VNN25_08440"
     CDS             complement(2025..2207)
                     /locus_tag="VNN25_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91595.1"
                     /translation="MSRTPAEIFQNPDKIIETPTAITPLQRGGLLLETERTPLQRELA
                     PLPAGGNASAASPLRT"
     gene            complement(2856..3239)
                     /locus_tag="VNN25_08445"
     CDS             complement(2856..3239)
                     /locus_tag="VNN25_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91596.1"
                     /translation="MDAATLWARAVRDRSGFRDAFFALLEEHSVRYCVVESNAYVEPL
                     IGLDLEVVIASEDLERIETLLEGQFHVERFEHSINVSLLDSQLREQIQTDPRYAGFVS
                     RAMAPEVLGFTLQVATVEDVLQGKL"
     gene            complement(3240..4283)
                     /locus_tag="VNN25_08450"
     CDS             complement(3240..4283)
                     /locus_tag="VNN25_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXH91597.1"
                     /translation="MSANSGRYPISERLKTPIAIVSERGAVEWSNAAFEATFGDDAGG
                     WLTEASRAVAGESGWLQGFFVDADELRSIDVDIEGRTYRIDKIMHVDDFDTPSVALWF
                     EDVTRQREAEQAKSDFTAQIVHDLRGPLSGIQGTLEFVLSQEGTKLDPLNSDLLTEAK
                     RESERMMGLINEIMDFSKIQSGSFAVEQEPVRVAGLLKRAVRSLQSVASRDEVFLLSA
                     HGRDVPQIIGSVEKLTQAVINLISNSLKFTPKKGLISVGAQILRKGDVAEAIVITVTD
                     SGMGIKATELQQIWDKYKQSGNKSLRGGGGTGLGLYIVKQVVEAHGGEVTVASVEGVG
                     TSMVLKLPVKRAA"
     gene            complement(4280..4693)
                     /locus_tag="VNN25_08455"
     CDS             complement(4280..4693)
                     /locus_tag="VNN25_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91598.1"
                     /translation="MSTLSPPKAPPVPDWSRLVADSIVQHGAWHTYMKLVEARGRYPD
                     DLSRLGYTEILRNMIVRDFLHHPKGMQAMPKLTPEFLSNFDRFNLTAQEGYLVSLIDG
                     RLDLQKLLLLSPFDHFTTLFNLAKLQHERAITVPQ"
     gene            4758..>6410
                     /locus_tag="VNN25_08460"
     CDS             4758..>6410
                     /locus_tag="VNN25_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXH91599.1"
                     /translation="MTDVLDLPPAAIPRSRRKSRLIILLLAAILGIPGGFLLAHAISV
                     PLVKSLENYQPAIITRIYDRNGIGFAEYSIQKRIVVAKRDMSPSVVQAIIATEDADFY
                     HHGGINPKAIFRAALKDLIARKKVEGASTLTQQLAKQVFLTPAKDFRRKINEAFLAVD
                     IEKNFTKDQIFELYANQVYLGHGAYGVEAASRLYFGKHAKDLTVPEAAMIAGLIRSPM
                     YYSPITHPDHALARRNHVLRRMFEEHYIPTKELLQQAVSSPIVLGTFKDEAPRIGAYF
                     AEEIRQHIEKSEKFGVENLYQHGLKVYSTLDLRMQQASETALQRGLRRWDRRRGFRKP
                     ARNLVAEGIDPNAYKDPSWSNDPYVTDKLYPAVVTVVDKAGVTLRVGKDSMTLPPASF
                     AWTQHKSMEGVLKTGDIIYIRLDEDSKTHARKWMLDQLPQVQGAVVIIDVKSGEVRSL
                     VGGYDFQISKFNRAIQSRRQTGSSFKPFVYGAAFEKGMTPADTIFDEPVAIPVGNQIY
                     SPKNYYGMYAGIVTIQRALELSINVPAVKTWMMVGADHVVDFA"
BASE COUNT         1316 a         1945 c         1902 g         1247 t
ORIGIN      
        1 gccgttgcgc gatcgattcg gtgagcggat tgctgcatcg ctcgatgcgg acgtgatcga
       61 gatcgaggaa ggggagtcgc acaagaacct cactaccgtg aacgacgtcg tcacgcagct
      121 tctcgcccgc ggagcgaagc gcgattcgat ggcggtggtg gtgggcgggg ggatggtggg
      181 ggatacggcg ggcttcgctg cgtcgatctt tctgcgcggc atcgatctcg tccacattcc
      241 gacaacgctc ctcgcgcagg tcgacagctc cctcggcggg aaggtcgcgg tcaatcacgc
      301 cagcggcaag aacctcatcg gcagtttctt tccgccgcga gcagtgatct cggatctgtc
      361 cacactcgac tcgctgccac gccgtgagat gctcagcggc ctcttcgaag cgctgaaggg
      421 tggcgtaatc ggcgatgcct cactcttcga gatgctcgaa tcgccgccgc gcatcgacgt
      481 cgacgaagtg gtgcgcaagg ccattcaggt gaaagcggag atcgtctcgg ccgatgagcg
      541 cgaagctgat ctgcggcgcc tgctcaacta cggccatacg atcggccacg gaatcgaagc
      601 cgctctcgac tatcgcaacc tcacccacgg cgaaggagtg gcttggggaa tgatcggcgc
      661 aaacgcgatc gctgtgcagc gcggactgct cgcgcgaaat gtcgccggaa ggatcgaccg
      721 cgccatccgc gcctggaacc cggcgccgat acctgcagac atcgatcgca atgccatcct
      781 cgcggcagcc gagcacgaca agaagaacac cggaagccag cgcgtcatgg tcttcccccg
      841 cgccatcgga acgtgcgagg tcttcgcgga catcaccgaa gcggagatcg cgttcggcgt
      901 cgatgcgatc ctcggtgaag ttcggtaata tctgccatgc gccgtcagcc gcccatatgg
      961 catttcgccg cggtatacga aacacgtctc gtgctgaatg agcgtctggt catcgccgtc
     1021 ccgccggtca aggtggaggt aattaccggg ccggcataca tgttccgctt gtcaaatgat
     1081 cgcgctgttg tgcctctaat gggtaaggtt ggcgaggagg tcgagattcc gtcaccgcca
     1141 gcgatcgacc tcgaagcagg taagcgctac atcattgtgt cgacgcgcac aaatgagaat
     1201 cctgctgtcg ttgaggaaga gtgccgccgc gtagtcgatc ggatgatagg gatcattggg
     1261 ctactctcat cgcctacgct ctttgcgctc ccgatttacg tcggttgggt tagcgcgaca
     1321 cccagcaccc cgttaaacac aaaagcaatg ttttctaagc cggaacaact aaaccgcgcg
     1381 gcgttggaaa aaggcgtctc cattgctcgt cgcgtactcg ctcgagaccc ggcaatgcgc
     1441 gatcgtttcg atcttgtcgc ccgacttttc agcagaacga tgggatcgcc tcccacggac
     1501 gaagcgtttc tttgggcgtg gacctgcctt gaggtctttc caatgatggg gacccagaag
     1561 catcgccata ttgcgccgta cctcgcgaag ctcacgggcg acgacgaggc agcacttgcc
     1621 gctcggctgg atatccgtgg gttatacatc ttgcggtcga aacttgtcca ttctgggcac
     1681 ctcggtcttg gcgatacgga gctgtacagg tcgctcacca tattgcgggg aatcgtctgc
     1741 acggtgatgc gaggcatgtg tggtctgcca tacgacggcg agcttgagcg tgtggttcag
     1801 ctgcgcgaac agccgaccgt cattccggtt tgagtcatgg ctatccaagg gaaggccgtc
     1861 gccttgaaag gatcacgacc gtgaggagcc ggcgtgtggt atcccagaga cgctacgccc
     1921 cccgcttcac cggcagctac agaccatgct cgtgcgacgc cacggtcaca tcggcgccag
     1981 ccttctgctg gcgctgaagg ctgcggaagc ggtgcgggta acgttcatgt gcgaagaggc
     2041 gaagcggcgg aggcattccc gccggccggc agcggagcaa gttcacgctg caacggcgtc
     2101 cgctccgttt ccagcagcag gccgcctcgc tggagcggcg tgatcgcagt cggcgtctcg
     2161 atgattttgt ccggattctg aaagatctca gccggcgtcc gagacattct gctcctcgga
     2221 gagatagctt gattccacct ggagaaagtt ctttcctgcg tagagaacgt tctttccgac
     2281 ccagagaaag ttctttccaa cctagagaaa cttctctcca acccagagaa agttctttcc
     2341 aacctagaga aacttctctc caacctagag aaacttctct ccaacctaaa gaaacttctc
     2401 tccaacgtag agaaacttct ctccaacgta gagaaagttc tctccgacgt agagaaagtt
     2461 ctctccgacg tagagaaagt ttcactccgc gtagagaagg tttcactccc catagagaaa
     2521 gtttcgctcc actgagagaa cgttacactc cacggagaga aggtttcact ccacggagag
     2581 aaggtttcac tccaagtaga gaaagtttca ctccaccaag agaaagtctt gctccaccca
     2641 gagaagattc tggtccggct ctgaaacgtt tgctccggtt gtctctgaac aaggacaacc
     2701 accacgacgc agcagcagcc acggactcag accagacggt cgaggacttc atcaggtacg
     2761 cggtcccgaa gtcggggata cgcttcgatc agtcgtacga tgtccgcgag atccttctga
     2821 cgcttgcttt gtcgacgggt gtcatcgaga aaggctcaga gctttccctg caacacatcc
     2881 tccacagtag caacctgcag agtgaaaccg agaacttcgg gcgccatggc ccgcgatacg
     2941 aatccggcat aacgcggatc ggtctggatc tgctcgcgaa gctgagaatc cagcaagctg
     3001 acgttgatgc tgtgctcgaa acgctcgacg tgaaactgtc cctcgagaag cgtttcgatc
     3061 cgctcaagat cttccgaggc gatgaccacc tcgagatcaa ggccgatcag aggctccacg
     3121 tatgcgttcg attcgacgac gcagtatcgg acggagtgct cctccagaag tgcaaagaac
     3181 gcgtcgcgaa agccggagcg gtcgcggaca gctctggccc acagcgttgc agcgtccacc
     3241 tacgccgccc gcttcaccgg cagcttcagg accatgctcg tgcccactcc ctcgactgag
     3301 gcgactgtca cttcgccgcc gtgggcttcg actacctgct tgacgatgta gagcccgagg
     3361 ccggtgccgc cgccgccgcg gagggacttg ttgccgctct gtttgtactt gtcccagatc
     3421 tgctgcagct ccgtggcctt gatccccatg ccgctgtccg tcaccgtgat cacgattgcc
     3481 tcggccacat cgcccttgcg caggatctgg gcgccgaccg agatcagacc cttcttcggc
     3541 gtgaacttca gggagttgga gatcaggttg atcaccgcct gggtcagctt ctccacgcta
     3601 ccgatgatct gcgggacgtc gcggccgtga gcggagagga ggaagacctc gtcgcgggag
     3661 gccacgcttt gcagactgcg cacggcgcgc ttgagcagac cggcgacgcg gaccggctcc
     3721 tgctcgacgg cgaaactgcc ggactggatc ttggagaaat ccatgatctc gttgatcagc
     3781 cccatcatcc gttcggactc ccgcttcgcc tcggtcagca gatccgagtt gagtggatcg
     3841 agcttcgttc cctcctgcga aagcacgaat tcgagcgtgc cctgaatgcc tgagagcggc
     3901 ccacgaagat cgtgaacgat ctgcgcggtg aagtccgact tggcctgctc ggcttctcgc
     3961 tgacgcgtca cgtcttcgaa ccagagcgcg acggagggcg tgtcgaagtc gtcgacatgc
     4021 atgatcttgt cgatccgata ggtgcgccct tcgatgtcga cgtcgatgga gcgaagctcg
     4081 tcggcatcga cgaaaaagcc ctgcagccag ccgctttcac cggcaacagc tcgtgaggcc
     4141 tcggtcagcc aacctccggc atcgtcgccg aacgtcgctt cgaacgccgc gttgctccat
     4201 tccacggcac cgcgttcgct gacgatcgcg atcggcgtct tgagacgctc ggagatcggg
     4261 tacctgccgc tgtttgcgct cattgcggca ccgtgatcgc gcgctcgtgc tggagcttgg
     4321 cgagattgaa cagcgtcgtg aagtgatcga acggactgag aaggagcagt ttctgcagat
     4381 cgaggcggcc atcgatgagt gacaccagat agccctcctg ggccgtcaga ttgaagcggt
     4441 cgaagttcga caggaactcc ggcgtcagct tcggcatggc ctgcatccct ttcggatggt
     4501 ggagaaaatc gcggacgatc atgtttcgca gaatctccgt gtagccgagg cgtgagaggt
     4561 catcgggata gcgtccgcgc gcttccacca gcttcatgta cgtatgccag gcaccgtgct
     4621 gcacgatcga atcggcgacc aaacggctcc agtcgggaac cgggggagcc ttgggtggag
     4681 agagggtcga catcggcgtc attctacaga gtccgtttag gaacgaggaa ggtgatccgc
     4741 tttgctacac tctaagtgtg acagacgttc tcgatcttcc tcccgcggcg atcccgcgca
     4801 gccgccgtaa gtcgcgtctg atcatccttc tgctggcagc catcctcggc atccccggcg
     4861 gattcctcct tgcccacgcc atcagcgtgc ctctggtcaa gtcgctggag aactatcagc
     4921 cggcgatcat cacgcgaatc tacgaccgca acggcatcgg attcgcggag tactccattc
     4981 agaaacggat cgtcgtcgcc aagcgcgata tgtcgccttc cgtcgtacag gcaatcatcg
     5041 ccaccgagga tgccgatttc tatcaccacg gcgggatcaa tccgaaagcg atcttccggg
     5101 ccgcgctgaa ggacctcatc gcccgcaaga aggtggaagg ggcatcgacg ctgacgcagc
     5161 aacttgcgaa gcaggtcttc ctgactccgg cgaaggattt ccgccgcaag atcaacgaag
     5221 cattcctcgc cgtcgacatc gagaagaact tcacgaagga tcagatcttc gagctttacg
     5281 cgaatcaggt ttacctcggc cacggcgcat acggcgttga agcggcgtcg cggctctact
     5341 tcggcaagca cgccaaagac ttgacggttc ccgaggcggc gatgatcgcg gggctgatcc
     5401 gctcgccgat gtactacagc ccgatcacgc atccggacca cgcgctggcc cggcgcaatc
     5461 acgtgctccg gcggatgttc gaggaacact acatcccgac gaaggagctg ctccagcagg
     5521 cggtgagctc gccgatcgtc ctcggcacgt tcaaggacga agcgccgcgc atcggcgcgt
     5581 acttcgcgga agagattcgc cagcacatcg agaagagcga gaagttcggc gtcgagaacc
     5641 tctaccagca cggcctcaag gtttattcaa cgctcgatct gcgcatgcag caggcctccg
     5701 aaacagcctt gcaacgcggc ctgcgtcgat gggacaggcg tcgcggattt cgaaagccgg
     5761 cgcggaatct ggtggccgag gggatcgatc cgaacgcgta caaagacccg agctggagta
     5821 acgatccata cgtcaccgac aagctctatc ccgcggtcgt cacggtcgtc gacaaggccg
     5881 gagtcacgtt gcgcgtcggc aaagactcga tgacgctccc gccggcgtcg ttcgcctgga
     5941 cccagcacaa gagcatggaa ggcgtcctca aaaccggcga catcatctac atccggctcg
     6001 atgaagattc gaagacgcac gcgcgcaaat ggatgctcga tcagttgccg caggtgcagg
     6061 gcgccgtggt catcatcgac gtgaagagcg gcgaagtacg ctcgctcgtc ggcggctacg
     6121 acttccagat ctcgaagttc aaccgcgcca tccagtcgcg ccgtcagacc ggttcatcgt
     6181 tcaagccgtt cgtctacggc gcggcgttcg agaagggcat gacgccggca gacacgattt
     6241 tcgacgagcc ggtagcgatt ccggtcggca atcagatcta ttcgccgaag aactactacg
     6301 gcatgtacgc cggcattgtg acgatccagc gcgcgctgga gttgtcgatc aacgtgcctg
     6361 cggtgaagac ctggatgatg gtcggcgccg accacgtcgt cgatttcgcg
//
LOCUS       DATLVW010000269         2820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_506757, whole genome shotgun sequence.
ACCESSION   DATLVW010000269 DATLVW010000000
VERSION     DATLVW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506757"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VNN25_08465"
     CDS             complement(<1..765)
                     /locus_tag="VNN25_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXH91600.1"
                     /translation="MQVTIDGRALVGNRTGIGVHTAEIAGRLSGSPLIASHAEIADRS
                     GLERCRFRVDPMALGVAWQQIVLPRTATDGVLWAPHGTLPLALRIPSVVTLHDFSSLT
                     MPLRHRIKTMLSFNLFIGRSLQMATRIAAVSSAVAEEAVRWFGVSRGRIEIVPNGVDE
                     FFTPDGDEDDYVLFVGTIEPRKGLDDLLAVWDSLPPPRPRLVLCGDAGWGNVTLPRDA
                     EVTGYVDRTRLRDLYRHARAFVYPSRYEGFGIPPLEA"
     gene            1109..2473
                     /locus_tag="VNN25_08470"
     CDS             1109..2473
                     /locus_tag="VNN25_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXH91601.1"
                     /translation="MAKRPRVLRSKRKIIGDLVVVNPDAAGIDAGSEEHWVSVPEDRS
                     DQPVRSFGTFTADLQALADWLVECRITTVAIEATGVYWIPLFEILEERGLNPKLVDSR
                     SIGSRKKKSDVVDCQWIRQLDTYGLLNGAFRPGADMLPLRALMRQRDMLIRYAADHIR
                     HIQKALDLMNVKLHLAVSDTVGVTGLRIIHAILSGKRDPMEMAALREPSCKSSEETFV
                     KALTGNYRAEHLFALKQAMDLFTTYQSKIKECDAQIEAALSKFDKTPATASLAPKPAK
                     RRRKNQPHFDGRTLLFEITGIDLTSVPSLEASTILTIISETGTDMSAWYNGKHFAAWL
                     NLSPNNRITGGKRIYKKMPVIRPNRAAQAFRIAAQTLERAQCALGAFFRRVRSRLGRS
                     GAIKATAHKLALIFYSMLKNKTQYREAGVDYYEARYRERLVNSLKKKAAEFGFNLTPI
                     QEVH"
BASE COUNT          602 a          874 c          851 g          493 t
ORIGIN      
        1 cgcctccaac ggcggaatgc cgaagccctc gtaccgcgac ggatagacga acgcgcgggc
       61 gtggcgatac agatcgcgca gccgcgtgcg gtcgacgtag ccggtcacct ccgcatcccg
      121 cggcagcgtc acgttccccc atcccgcatc gccgcacaga acgagtcgcg gccgcggcgg
      181 cggcagcgag tcccacaccg ccagcagatc gtcgagccct ttgcgcggct cgatcgtgcc
      241 gacgaagaga acgtaatcgt cctcgtcacc gtccggtgtg aaaaactcat cgacgccgtt
      301 cggcacgatc tcgatccttc ccctggacac gccgaaccac cttactgcct cctcggccac
      361 cgcactcgac accgcggcga ttcgagtggc catctgcagg ctccgtccga tgaagaggtt
      421 gaacgaaagc atggtcttga tgcgatggcg caacggcatc gtgagcgagc tgaaatcgtg
      481 aagagtgacc accgagggga tgcggagcgc cagggggagc gtcccgtgcg gcgcccagag
      541 cacgccgtcg gtggcggtgc gcggcagtac gatctgctgc caggccacgc cgagcgccat
      601 cggatcgacg cgaaaacggc agcgctcgag gcccgagcgg tcggcgatct ccgcgtgcga
      661 cgcgatgagc ggcgagccgc tcagccgtcc cgcgatctcg gccgtgtgaa cgccgatgcc
      721 ggtccgattg ccgaccagcg cgcggccgtc gatggtgact tgcatgttgg tgaggtcagt
      781 gtagcgacca gattggatca agagtacgcc gcacaacaaa tagcaatgtc gcgcaacaaa
      841 tagcaatgtc atcctgagcg ggttaacggc gcgagcgccg ttgatgaggg aatgaaccgg
      901 tatcctgcac gtgcggtccc gcagacgagg ccgtcaagac agtggtgcgc agtacgagcc
      961 cgttcctgag agtggtcccg gaaggccgaa gtcgccttgg tgacgcgaca cggttaggag
     1021 ccgcgttcgc gatcccgttt agaagagtca tttaagctgg gccttccgct gcgggtccgc
     1081 aaaaatttca ttttgcggag gtgttgtcat ggcaaagcga cctcgggtcc tccggtccaa
     1141 acgcaagatt atcggagatc tggtcgtggt caacccggat gcagcaggaa tcgacgccgg
     1201 gtcggaagag cactgggtgt cggttcccga agatcgcagc gatcagccgg ttcgctcatt
     1261 cggaacattc acggccgatc tgcaggcact ggccgactgg cttgtggaat gccggatcac
     1321 cacggtggcc atcgaagcga cgggggtgta ttggattccc ctattcgaga ttttggagga
     1381 gcgcgggctg aacccgaaac tggtcgactc gcgaagcatc ggctcacgaa aaaagaagtc
     1441 ggacgtggtc gactgccaat ggatccggca actggacacg tatggtttgc tcaacggagc
     1501 tttccggcca ggtgcagaca tgcttccgct ccgggcgttg atgcgtcagc gagacatgct
     1561 gatccggtac gccgctgacc atatccggca cattcagaaa gctctcgatc tgatgaatgt
     1621 gaagcttcac ctggccgttt ccgacacggt tggcgtcacc ggactgcgga tcattcacgc
     1681 gatcctttcc ggaaaacgtg atccgatgga gatggcggca ttacgcgaac cgagttgcaa
     1741 aagctccgag gaaaccttcg tgaaggcgtt gacaggcaac taccgcgctg agcatctgtt
     1801 cgcgctcaaa caagcgatgg atctgttcac cacctaccag agcaagatca aagagtgcga
     1861 tgcgcagata gaggctgcgc tttcgaagtt cgacaaaacg ccggccacgg ccagtctggc
     1921 acccaagcct gcaaaacgcc gtcgcaagaa ccagcctcac ttcgacggac ggactctcct
     1981 gttcgaaatc accggcattg atctcacgtc ggttccctcg ctggaggcct ccacgatcct
     2041 gaccatcatc tccgaaactg ggacggacat gtctgcctgg tacaacggca aacacttcgc
     2101 agcttggctc aacctcagcc cgaacaatcg catcaccggc ggaaaacgaa tctacaaaaa
     2161 aatgcccgtg atccgaccaa accgcgctgc tcaggccttt cgaatagccg ctcaaactct
     2221 cgaacgagcc cagtgtgctc tcggagcatt cttccgccgg gtcagatccc ggctgggccg
     2281 ctcaggcgcc atcaaagcaa ccgctcacaa gctcgccctc atcttctact ctatgctcaa
     2341 gaacaaaacc caataccgcg aagcgggtgt cgactactac gaggctcgct atcgcgaacg
     2401 tctcgttaac tccttgaaga aaaaggccgc cgagttcggc ttcaacctca ctccgattca
     2461 ggaggttcat tagaagaagg ctgccggagc cgcgtagccg aaggcggagc tggcggagga
     2521 cgccgaagga cccccagctt gctaacctgg acagggcggt ggaatgtcga ctattccgcc
     2581 gcccaaccgg gatacaactt tggggtcctt cgaccgtcct ccgccgcgcc gcgctccgcg
     2641 cggcgaggct ccggcggctc aggaagacag tgatgtgatg acggcgtggg gcaacaaaca
     2701 acgccttcgg ctcgcaatgc gatagccttc cccgcgcatg aaactcgtcg aatgtgttcc
     2761 gaacatttcc gaggggcgtc ggcccgagat ctatgacgcc gtggccgcgg ccgcggcggg
//
LOCUS       DATLVW010000270         3271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_589421, whole genome shotgun sequence.
ACCESSION   DATLVW010000270 DATLVW010000000
VERSION     DATLVW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589421"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VNN25_08475"
     CDS             <1..1444
                     /locus_tag="VNN25_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HXH91602.1"
                     /translation="EVRNPDGSVHAEHRRAAEPFVRFLTTLSAGGPASEDYDVPPQPL
                     VPETAPPALGRVMPMVAALATFSASSYTFTVSGSLGPRWFVFPNAVSFTSLGTEPGAP
                     GGGVTAINTAMAAWNNDPNSNVNYVYAGADNGTHTAGLSGSDGANTIAFERDLSSFGV
                     TPFQCSANGYSGTLGIGGVTKTSGTGAGPNNETFAVTAEGDVEMNQGIANCTLLFNNG
                     DFNSAVTHEVGHTLGFRHSDQTRADSSGTACTTDPSLECADVAIMKSFIPNGLNAALQ
                     PWDQHAVAAVYPGSGTQPPPAPTGVNARATTSTQVTITWNAATGATSYQIFRRAPGGS
                     FTQIGTSTTTSFVDNTASANTAYLYRVRAVNAGGASSDSAADLATTVIFTDDPLTVRS
                     TRIKAVHLAELRTAVNAVRSLAGLTAFSFTDSASAGVLIKATHINQLRTALDAALSPL
                     GFATGGYTDTLSAGVPVKAVHVQEIRNRVK"
     gene            complement(1496..>3271)
                     /locus_tag="VNN25_08480"
     CDS             complement(1496..>3271)
                     /locus_tag="VNN25_08480"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH91603.1"
                     /translation="HGNAIALLPFVMLGARMVARSPGVRAASLLAGVLILLTLCGHPE
                     TSLHVIALATVYLIFCAREKWGLVIVSGLGAGIAALLLTAFFLLPMIEAIPQTREYLH
                     RRSGAETMSASWSGVAHALVIDVIPFGDGTPGIEETRHDAPLRHRSLGSAYCGAMLLA
                     PALLALLRSRSRERYFFLGVVLFGLLTGAEAPGVTGLLAHLPVFNLAINARLISFAAF
                     GICVLGAIGLEESIAQRHTLAWLFLGAALTITAGTVFVALEGTLTPDFVRANAIREVV
                     PLLLAFAVLRAFTMHRLVMGGLLALLLLQRVMEFGGFIATVDRRAFFPPIAGLEQLPR
                     NSEPYRIVAQGPLFAPNISVLYGLEDVRGYQAMTLARFAETFPLWSIPQPVWSNRVDD
                     LTAPMLSLMNVRYALALPETVPPASWRLIGRYPGYQLIENSGVLPRAFIPRVIHSGAN
                     NVLAGMASCRDFASEGWIETEGAPANVANGEGTVAVHALGSHVTLHASMRSPGWIVIS
                     ETFWKGWSATMGRQRLKVHFADHAFLGIYIPAGVHDIELSYRPRSVTTGAIISIVSAL
                     AMIAVVVLGRRRNEESTTETLRTQS"
BASE COUNT          671 a         1087 c          995 g          518 t
ORIGIN      
        1 ggaggtgcgc aatcctgatg gttcggttca tgccgagcat cgccgcgccg ccgaaccttt
       61 cgttcgattc ctcaccaccc tctccgccgg tggtccggca agcgaggatt acgacgtacc
      121 gccgcagccg ctcgttcctg agactgcgcc gccggcgctc ggccgcgtga tgccgatggt
      181 cgcggcgctg gccacctttt cggcaagctc gtacaccttc acagtgagcg gatcactcgg
      241 accgcgctgg tttgttttcc ccaacgcggt cagcttcacc agtctcggca ccgagcccgg
      301 cgcgcccggc ggaggagtca ccgcaatcaa tacagcgatg gcggcctgga acaacgaccc
      361 caactcgaac gtgaattacg tctacgccgg agccgacaac ggcacacaca ctgcgggcct
      421 gtccggatca gacggcgcca acaccatcgc cttcgagcgc gatctttctt cgttcggcgt
      481 gacaccgttc cagtgctctg cgaacggcta cagcggaacg ctcggcatcg gcggcgtgac
      541 gaagacctcg ggcacgggcg ccggtccgaa caacgagacc tttgcggtaa ccgcggaagg
      601 cgacgtcgag atgaaccagg gcatcgccaa ctgcacgctg ctcttcaaca acggcgactt
      661 caactccgcg gtcacccacg aagtcggaca cacactcggg ttccggcact ccgatcagac
      721 gcgggcggat agttcgggta ccgcgtgcac cacggatcca tcgctggaat gcgcggacgt
      781 agcaatcatg aagtcgttca tcccgaacgg tctgaatgca gcgctgcagc cgtgggatca
      841 gcacgcggtg gcggctgtct accccggctc cggcacgcaa cctccgccgg ctccgaccgg
      901 cgtcaatgcg cgagcgacga cgtcgacgca ggtgacgatc acatggaacg ccgccacggg
      961 tgcgacgtcc taccagatct tccgccgcgc tcccggcgga tcgttcacgc agatcggaac
     1021 atcgaccacg acgtcgtttg tcgacaacac cgcgtcggca aacactgcct acctctatcg
     1081 cgttcgtgcc gtgaacgcag gcggtgcgtc atccgacagc gcggcggatc tcgcaacaac
     1141 cgtcatcttc accgacgacc cgctcacggt tcgctcgacc aggatcaagg ccgtgcatct
     1201 cgcggagctg cgcaccgccg tcaacgccgt tcgctcgctg gcgggcctca ccgcgttcag
     1261 cttcacggac agcgcatcgg cgggcgtgct catcaaagca actcacatca atcaactaag
     1321 gacggctctc gatgcggcgc tctcgccact cgggttcgcg accggcggct acaccgatac
     1381 gttgagcgcg ggcgttccgg tcaaggccgt gcacgtgcag gaaatccgca atcgcgtgaa
     1441 gtgaggtaac aaaagctttg aaccacagag acacagaggt cacagagtaa cggcttcaac
     1501 tctgtgtcct cagtgtctct gtggttgatt cttcgtttcg tcttcggccg aggaccacga
     1561 ctgcaatcat cgcaagcgcc gagacgatac tgatgatcgc gcccgtcgta acggacctcg
     1621 gtcgatacga cagctcgatg tcgtgcacgc cggcaggaat gtagatgccg aggaaggcgt
     1681 ggtcggcgaa gtggaccttg agacgctggc gtcccatcgt tgcgctccag cctttccaga
     1741 acgtttccga aatgacgatc catccaggcg agcgcatcga ggcatgaagc gttacgtgcg
     1801 atccgagtgc gtgaacggcc acagttcctt cgccgttcgc cacgttcgcc ggcgctccct
     1861 cggtctcgat ccacccctcg gacgcgaagt cgcgacaaga agccataccg gcgagcacgt
     1921 tgttcgcgcc ggagtgaatc acccgaggga tgaaggcgcg gggaagcacg ccgctgttct
     1981 cgatcagttg atagccagga taacgtccga tgagacgcca gcttgcaggc ggtaccgttt
     2041 ccggcagtgc cagcgcgtag cgcacattca tcagtgacaa cattggagcg gtcagatcgt
     2101 cgacgcggtt cgaccagacc ggttgcggga tcgaccagag cggaaacgtc tcggcaaacc
     2161 tcgccaacgt catcgcctga taaccgcgta catcctcgag gccgtagagc acgctgatat
     2221 tcggcgcgaa cagcggccct tgcgccacga tgcggtacgg ctcgctgttc cgaggaagtt
     2281 gctcgagacc ggcgatcggc ggaaagaacg cgcggcggtc caccgtggcg atgaagcctc
     2341 cgaactccat gactcgctgc aacaagagaa gcgcgagcag tccgcccatc accagacgat
     2401 gcattgtgaa cgcgcggagc accgcgaacg cgagcagcag cggcacgact tcgcgaatgg
     2461 cgttggcgcg aacaaagtcg ggagtgagcg tgccttcgag cgcgacgaac acggtcccag
     2521 ccgtgatcgt cagtgcggcg ccgaggaaca gccacgccag cgtatgacgc tgtgcgatcg
     2581 actcctccag accgattgca ccaagcacac agatgccgaa tgccgcgaac gagatcagcc
     2641 gcgcgttgat ggccaggttg aataccggca aatgcgccag gagaccggtc acgccgggag
     2701 cctccgcacc ggtcagcagc ccgaacaaaa cgaccccgag gaagaagtac cgttcgcgac
     2761 tccgtgagcg caggagcgcc agaagagccg gagccagcaa catcgccccg caatacgccg
     2821 accccagcga acgatggcgc agcggcgcat cgtgacgcgt ctcctcaatg cccggcgttc
     2881 cgtcgccgaa cggtatgacg tcgatcacca acgcgtgcgc gaccccactc cacgatgcgc
     2941 tcatcgtctc ggcacccgat cgccggtgca ggtactcgcg cgtctgcgga atcgcctcga
     3001 tcatcggcaa cagaaagaat gcggtgagga gcagggcggc gatgccggcg ccgaggccgc
     3061 tgacgatcac gagtccccat ttctcacgcg cgcagaaaat cagatagacc gtggccaggg
     3121 cgatgacgtg aagcgaagtc tccggatgac cgcacagcgt cagcaggatc agcacaccgg
     3181 ccaggagcga cgccgcgcgt actcccggac tgcgcgccac catccgtgcc ccgagcatga
     3241 cgaacgggag cagcgcgatc gcgttgccgt g
//
LOCUS       DATLVW010000271         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_259216, whole genome shotgun sequence.
ACCESSION   DATLVW010000271 DATLVW010000000
VERSION     DATLVW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259216"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VNN25_08485"
     CDS             <1..561
                     /locus_tag="VNN25_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH91604.1"
                     /translation="FTNAIDTRTNGYDLVVNHQQPLLNGRIDLSAAYSNNKTRIVDIA
                     PTPTQLTGLGAVLFDRGEQRRTECGQPRDNTRLMESYNQGAVNITARESRYGSFCSAT
                     INPVDDQTYSPKWLTDLEMSYRWNRYTFALGAENLFDTFPDRNLRAGTPSGSAQVGSA
                     GVFLYPINSPFGMNGRFVYTRVSYSF"
     gene            complement(500..758)
                     /locus_tag="VNN25_08490"
                     /pseudo
     CDS             complement(500..758)
                     /locus_tag="VNN25_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(813..2048)
                     /locus_tag="VNN25_08495"
     CDS             complement(813..2048)
                     /locus_tag="VNN25_08495"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH91605.1"
                     /translation="MTAASMPVSAEPAVQVHPLRNANFVLWWLGATTSLLGDQFYLVA
                     LPWVVLQLTGSGVAMGTVAMCAGIPRAVLMLMGGAITDRTSPRKILMATASARAVLVA
                     AIGFLLLRNVLQLWHLYALGLAFGVADAFALPSSGPLLRSLVKQEQLPAANSVWQSSA
                     LLTGIVGPAPAGMITKALGAAWAFFLDAFSFLFVIAALWKVPDPPRSSSTAAKPGVWS
                     AIGEGLAYVMKDVPLRSLLLLAAVLNFCLSGPLSVGLAFIAKSRFSSPAAFGGWISSV
                     AAGTLVGMILAGIFKSRRRGLLLLGTSTVLGFLTALMGLLAGFWPVALLLLVMGCFSG
                     FINVQIQAWLQQRVERAVLGRVSSVAMLSSFGLMPLSMGAAGIAVDWSARWMFAIAGA
                     AVALVAAFGALQRPLREME"
BASE COUNT          474 a          702 c          683 g          338 t
ORIGIN      
        1 ttcaccaacg caatcgacac tcgcacgaat ggctacgatc tcgtcgtcaa ccatcagcaa
       61 cccctgctga acggccgcat cgatctttcg gcagcgtaca gcaacaacaa gacgcggatc
      121 gtcgacatcg cgccgacccc gacgcagttg accggcctcg gagccgtgtt gttcgatcgc
      181 ggcgaacagc gccggaccga atgcggtcag ccgcgcgaca acacccgtct catggagagt
      241 tacaaccagg gcgccgtgaa catcacagcc agagagagcc gttacggcag cttctgcagc
      301 gcgacgatca atccggtcga cgatcagacc tattcgccga aatggctcac cgatctggaa
      361 atgtcctacc gctggaacag atacacgttt gcccttggcg cggagaatct gttcgatacg
      421 tttcccgaca gaaacctgcg ggccggcact ccatcgggct ccgcgcaggt gggaagcgcc
      481 ggcgtgtttc tgtatccgat caactcgccg ttcgggatga acggacggtt cgtgtatacg
      541 cgggtgagct atagcttctg accaggcacg gtgacgccgt cgcgagcagg ctcggcggca
      601 tcatcgcttc gaaagcacgc tttgcctcat cgctgccggc cacgagcgcg tcggtcagaa
      661 cgcgtgggcg atctctgcca ggacaggccc cagcggtcct tgcaccaagc atattggctt
      721 tcctgcaact cgttgttgat gatcgcattc caatagcggc tctatacggc gcgagggacg
      781 tggccagaga gatccgaggg gaagtctcgc cgctactcca tttcccgcag gggtcgctgg
      841 agcgcaccaa atgcggccac gagagcgacc gccgcaccgg cgatggcgaa catccaccgt
      901 gcgctccagt ccactgcgat gccggctgcc cccatcgaga gcggcatcaa accgaacgac
      961 gacagcatgg cgacgctgct gacgcgcccc agaacggcac gttccacacg ctgctgaagc
     1021 caggcttgaa tctgtacgtt gatgaacccg ctgaagcacc ccatcaccag aagcagcaag
     1081 gctacgggcc agaaaccggc cagcagaccc atcaaggccg tcaagaatcc gaggacggtg
     1141 ctcgtgccca ggagcaggag cccgcggcgg cgcgacttga agattccggc caaaatcatt
     1201 cccaccagcg ttccggcggc cacgctggaa atccatccgc cgaatgcggc cggcgaggag
     1261 aacctgctct tggcgatgaa cgccaagccc acgctcagcg gtcccgacag gcagaagttc
     1321 aagaccgcgg ccagcagcag gagcgagcgg agaggtacgt ccttcatgac gtaggcaaga
     1381 ccttcgccga tggcgctcca gacgccgggc ttcgccgcgg tggaagaaga acgtggcgga
     1441 tccggcacct tccacagcgc ggcgatgacg aacaggaagc tgaacgcatc gagaaagaag
     1501 gcccaggccg cgccaagcgc cttcgtgatc atgcccgcgg gggccggccc gacgattcct
     1561 gtcagaaggg cactgctctg ccacaccgag ttggcggccg gaagttgttc ctgcttgacc
     1621 agagaccgca gcaggggtcc cgacgatggc agagcaaagg catcggcgac gccgaacgcc
     1681 aagccgagcg cgtagagatg ccacagctgc aagacgttcc tcagaaggag gaacccgatc
     1741 gcggccacga gcacggcccg cgccgacgcg gtggccatga ggatcttgcg tggcgaggtc
     1801 cggtcggtga tcgcgccgcc catcagcatc aacacagcgc gcgggatgcc cgcgcacatc
     1861 gcgacggtcc ccatcgccac gcccgagccg gtcaactgga gcacgaccca cggcagggcg
     1921 acgaggtaga actggtcccc gagcagggac gtcgtcgcgc ccagccacca gaggacgaaa
     1981 ttggcgttgc gcagtggatg gacctgaacg gcgggttcgg cggacacggg catgctggca
     2041 gcagtcatgg ctatctcctc gttctcatcg atgggcgctt gtcataatgc gaatgtgaat
     2101 ttacatttac atcagccgga tcgtcaagag attttcctgt cgcgcttttg gcgggaggcg
     2161 ggtatataag gaggcgatgc cgaagccgga gaagacc
//
LOCUS       DATLVW010000272         7793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_589483, whole genome shotgun sequence.
ACCESSION   DATLVW010000272 DATLVW010000000
VERSION     DATLVW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589483"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(567..4646)
                     /locus_tag="VNN25_08500"
     CDS             complement(567..4646)
                     /locus_tag="VNN25_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH91606.1"
                     /translation="MSDGKGIKETFKEQAKAHGKATAKKIKGLIKSLSPEQRDLLLRR
                     LTEQRPAGPVVRAEGDDVPLSFAQEREWFRDRLFPDVAHNISGALRLEGTLSIDALQA
                     TFDAIIARHETLRSNITEVSGTPVQTVRAATSLPIRVVEASDWRALYAEEMVRGFDLA
                     NDLLLRVTLIRVADHEHVLLVTMHHIASDGWSIGVLMAEIAELYAAFVQQRPSALPTL
                     PIQYGDFARWQRERNLEDGLAYWRAQLADVPPALELPPDFAVNSSGLDHDAGSISSQV
                     GGSLKQNLEALSKGSETTLFVTLLTAFMVLLMRRTGSEDIVIGSPVSGRTRVECEPLI
                     GLFLNTLALRAKMSPDLSFREAMTIVRQTVLDALAHADVPIERVVQDLDIRRSSGVHP
                     LYETIFNFTPSAPRRLDLPGLRVTLEDAPALIEEFSTQLFITDFDGSLSLDLRYRGRR
                     YSEGLMASFLDQYIRLLEQAAVEPERRIGSFDLTTALSPDPTIRLEKPRYPTVVECIA
                     DWIERTPDALAVSQSGGGLTYAEFGAAMRNVADSLRALGLHRGDVVAVFGTRAPSLIA
                     AMAGVFSAGGVLLTLSPDLPEQRRKVMLDEAGARFLIAGGEIVELPGAGSGARADDAA
                     YVFFTSGSTGTPKAILGTHQGLAHWLHWQRETFEVGPGDRSGQLTGLSFDVVLRDIFL
                     PLTSGATLVLPDDDDILGWLDREQITTIHTVPAVAETWLLRRREDVTLRSLRRIFFAG
                     EPLTDVLIGRWREAFLQSGEIINLYGPTETTLAKCFYRVPPNPRAGIQPLGEAIPHTQ
                     VLVLTPERTLCGAGEPGEIAIRTPFRSLGYMNAAGENAKRFVRSPFTADENDILYLTG
                     DRGAFGADGRLEFRGRIDHQVKIRGVRVEPMEVAATLQAHADVAACAVIARADGPASG
                     EQILVAYVVLQENVDGSSGRLQEYLAQRLPAAMVPSAIVFLDALPLTANQKLDREKLP
                     APAGVRAALESRYVAPRDAIELRLVQIWEELLRVRPVGVTDRFFEIGGHSLLALRLLV
                     EVEQRLGRKVPLQALFEEPTIEHMAAVLRRNSGEWPLMVTLRPGEQRIKLFLVHPGGG
                     TLLNYVHLVRHLPTEIPVYGIQARGLDGTGEPHDTLEQMAADYIAEIRSAQPDGPYLL
                     VGHSLGGVIAFEIARQLCLKNERVAFLGLFDSVAPLSSGENTVSDSRREDALRLATMA
                     ETIGRFLGERVDVSIDELSNLGTDEQIDHVVDALRNSRAVPPGEEEKLVRNLLKVSKA
                     HIRAHRTYQPEIVPVPITLYRAGEAQPADYPSARIELLQKESLGWDSLTTEPVRVVQT
                     SGNHVTMLSSSHAEELAHLLRLSLSEAIAKEGEARLQNA"
     gene            complement(4639..>7793)
                     /locus_tag="VNN25_08505"
     CDS             complement(4639..>7793)
                     /locus_tag="VNN25_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH91607.1"
                     /translation="LEEAPVVSEPSPEARPFDLLLLSARTPTALDAATNRLADHLQAD
                     QAARLSDVAYTLRHGRRAFSERRFAVAMDPDDAVAALRDPQRIVTGSAPAGRPSIAFM
                     FPGLGDHYPGMGWELYCTEPVFRDSVDRSAALLKNHLAGDIRDFLYPDRHWSHPRLEP
                     PTKSKGSLDLRAMLGKKRVAPDDQPVAAQPAIFVTEVALAELLRSWGLEPEAMIGHSI
                     GEFTAAHLSGVLSLADAVEVVAARARLIQSHVTPGAMLAVPLTEAELLPLLPSGVSLG
                     AINAPRLCIASGDESGIAELAATLGGRDISCQRLRSTHAYHSSMMEAIVEPLLAVLRR
                     VALHAPRIPYVSCITGTWITDAEATDPGYWARHLCRTVRFADGLSTLLDDPRRVLFEA
                     GPGQGLTSHAIAQKRENAIIPSMRWSYGLQSELEVLLRGVGQLWLAGASLDTARFLTT
                     PDPRRVRLPSYPFERRRYWIDPPAADAPPSAHRKDDVAEWFYLPSWKPSVRARRREDF
                     SGSWLIVADRYGIAEELARALRARGASVEIGHDADGVPDDGRRIIIHLASLTPRDDAA
                     PSAERFAEMQDLGYHSLMELLRGVQGAANIEIVTNHVDDIPEKATLRAPAMVAPQEHP
                     NLVCRMIETDLDVGRRDALVAQILDEICSEPDDSLVAYRRGSRFVPAYEPVKLASSAT
                     VLRERGVYLITGGLGGVGMVLARHLAERVQARLVLIGRSAPTDEKLRQLAELEDLGAE
                     VLVLAADVSSRDDMQHVLAAVDERFGELHGVIHAAGAVGIETFREIREATPADSEPQF
                     VAKVKGLLVLDEVLAGRTPDFCLLMSSLSAILGGLGFSAYSAANLFMDSFARWKNRDG
                     ATGWTSVDWDSWRLADTRPVIAGLGATVSEFVMEPHEGADAFERILAAGGLGQVVVSS
                     GDLQARLRRWIGHRDELDAAPLIQHERPALRTAYEPPRGEIESELASIWQELFGVSTV
                     GVNDNFFELGGHSLLATQLNARLSLKLHVEMSLAALLQAPTIAQLAIAVVTHQAEQAD
                     PELLELMLAEVGGLSGDGDE"
BASE COUNT         1348 a         2534 c         2688 g         1223 t
ORIGIN      
        1 gccgcacgtg cggacaggaa gagccgcaat gcggacaact agcgccgcac gtgcggacag
       61 ggagagccgc aatgcggaca actagcgccg cacgtgcgga caggaagagc cgcaatgcgg
      121 acaactagcg ccgcacgtgc ggacaggaag ggccgcaatg cggtcaacta gcgccgcacg
      181 tgcggacaca tcgtgccgca acgttgcgcc tcggcgcgca cgtgcgcgta tgttgctgca
      241 aacgttacac ctcggcgcgt ccccggggct aaccctgcgc taacccgttt tctcacaacc
      301 ctatcgcgac tctctccaat cgagcgcctt ctccccccgt gcttttcggg gggagaaggt
      361 gccgaaggcg gatgaggggg gcgcaagatg ggagaaacac gttgttcgcg agcgtgaacc
      421 gattgacctg ctcagtgcct ttgcaacgtt gaagcgacag ccaaacgccc cctcatccgg
      481 ccttcggcca ccttctcccc cacaaacagc gtgggggaga aggcgctcga ttggaaagag
      541 tcgcaattag agagtgagaa gtacggctaa gcgttctgaa gccgcgcctc tccttccttt
      601 gcgatcgctt cgctgagcga cagccgcagc aagtgcgcca gttcctcggc gtgcgaggag
      661 ctgagcatcg tcacgtgatt gccggaggtt tgcaccaccc gcaccggctc cgtcgtcagg
      721 gagtcccagc cgagcgactc tttttgcagc agctcgatgc gcgccgaggg gtaatcggca
      781 ggctgtgctt cgccggcgcg gtagagcgtg atcggaacgg gaacgatctc cggctgatac
      841 gtgcgatggg cgcggatatg cgccttgctg actttgagga ggttgcggac cagcttctcc
      901 tcctcgcccg ggggcacggc gcgcgagttg cggagggcat cgacgacatg gtcgatctgc
      961 tcgtcggtgc cgaggttgga gagctcatcg atcgagacgt cgacgcgctc gccgaggaag
     1021 cggccgatgg tttcggccat cgtggccagg cggagcgcgt cttcacgccg gctgtcggac
     1081 accgtgttct cgccggacga cagcggagcg acggagtcga agaggccgag gaaggcgacg
     1141 cgctcgttct tgaggcagag ctgccgcgcg atctcgaacg cgatcacacc gccgagggaa
     1201 tgaccgacca gcagatacgg cccgtccggc tgcgccgagc ggatctccgc gatgtagtcg
     1261 gcggccatct gctcgagcgt gtcgtgcggc tcgccggttc cgtcgaggcc tcgcgcctgg
     1321 atgccgtaca ccggaatctc ggttggcagg tgtcggacca gatggacgta gttgagcagc
     1381 gtgccgccgc ccggatggac gaggaagagc ttgatgcgct gctcgcccgg ccggagcgtg
     1441 accatcagcg gccactctcc cgaattgcgg cgcagcaccg cggccatgtg ctcgatggtc
     1501 ggctcttcga agagcgcctg cagcggcacc ttgcggccga ggcgctgctc gacttcgacg
     1561 aggagccgca gcgcgagcag cgagtgcccg ccgatctcga agaagcgatc ggtgacgccg
     1621 accgggcgca cgcgcagcag ttcttcccag atctgcacga gccgcagctc gatcgcgtcg
     1681 cgcggcgcga cgtaacggct ttcgagcgcc gcgcgcacgc ccgcgggcgc aggaagcttc
     1741 tcccgatcga gcttctggtt cgccgtgagc gggagcgcgt cgaggaagac aatcgcggaa
     1801 ggcaccatcg cggcgggaag ccgctgcgcg agatactcct gcagtcttcc ggagctgccg
     1861 tctacgttct cttgcagcac gacgtacgcg acgagaatct gttcgcccga cgcgggtccg
     1921 tccgcgcgcg cgatgacagc gcaggcggcg acgtcggcat gcgcctgcag cgtcgcggcg
     1981 acttccatcg gctcgacgcg cacgccgcga atctttacct gatgatcgat gcgtccgcgg
     2041 aattcgagcc ggccatcggc tccgaacgcg ccacgatcgc cggtcagata aaggatgtcg
     2101 ttctcgtccg cggtgaacgg gctgcgaacg aaccgcttcg cgttttcgcc ggccgcgttc
     2161 atgtagccga ggctgcggaa gggtgtgcgg atcgcgatct cgcccggctc gccggcaccg
     2221 cagagcgtgc gctcgggtgt gaggacgagc acctgcgtgt gcggaatcgc ttcgccgagc
     2281 ggctgaatgc cggcgcgcgg attcggcggc acgcggtaga agcacttcgc gagagtcgtc
     2341 tccgtcgggc catagagatt gatgatctcg cccgattgca ggaacgcctc gcgccagcgg
     2401 ccgatcagca catcggtgag cggctcgccg gcgaagaaga tccgccgcag tgagcgcagc
     2461 gtgacgtctt cccgcctgcg cagcagccag gtctcggcga cggctggaac ggtgtgaatc
     2521 gtcgtaatct gctcgcggtc gagccagccg agaatatcgt cgtcgtccgg caggacgaga
     2581 gtggctcccg aggtgagggg aaggaagatg tctcgcagca cgacgtcgaa cgagagtccc
     2641 gtcagttggc cggagcgatc gccgggaccg acttcgaacg tctcgcgttg ccaatggagc
     2701 cagtgcgcga ggccctgatg cgtgccgagg atcgctttcg gagtgccggt actgccggag
     2761 gtgaagaaga cgtatgcggc gtcgtccgcg cgcgctccgg aaccggcgcc ggggagctcg
     2821 acgatctcgc cgcccgcgat caggaatcgc gcgcctgctt catcgagcat caccttccgg
     2881 cgttgctcgg ggagatcggg actcagcgtc agcaatacgc cgcccgcgga gaagacgcca
     2941 gccatcgcag caatgagcga cggcgcgcgc gttccgaata cggcgacgac atcgccgcga
     3001 tgcaggccga gtgcacgcag cgaatcggcg acgttgcgca tcgccgcgcc gaattcggcg
     3061 tacgtcaatc cgccgccgct ctgcgagacc gcgagggcat ccggcgtgcg ctcgatccag
     3121 tccgcgatgc actcgactac cgtcgggtac cgcggcttct cgagccgtat cgtcgggtcg
     3181 ggtgagagcg ccgtcgtcag atcgaatgag ccgatgcgcc gctcgggctc gactgcggcc
     3241 tgctcgagca ggcggatgta ctggtcgagg aagctcgcca tcagcccttc ggagtagcgc
     3301 cggccgcggt agcgcagatc gagcgacagg ctgccgtcga agtcggtgat gaagagctgg
     3361 gtcgagaact cctcgattag cgccggcgcg tcttcgagag tgacgcggag accgggcagg
     3421 tcgagccggc gcggcgcgct cggcgtgaag ttgaagatcg tctcgtagag cggatgaacg
     3481 ccggagctgc ggcggatgtc gaggtcctgg actacgcgct cgatcgggac atcggcgtgc
     3541 gcgagcgcat cgagcacggt ttgccggacg atcgtcatcg cctcgcggaa actgagatcc
     3601 ggcgacatct tcgcgcgcag cgcgagagtg ttgaggaaga ggccgatcag cggctcgcac
     3661 tcgacgcgcg tgcgtccgct caccggcgag ccgatcacga tgtcctcgct ccccgttcgc
     3721 cgcatcagca ggaccatgaa cgcggtcagc aacgtgacga acaacgtcgt ctcgctaccc
     3781 ttgctcaggg cctcgagatt ctgtttcagc gatccgccca cttgcgacga gatcgagccg
     3841 gcgtcgtgat cgaggccgct cgagttgact gcgaagtcgg gcggaagctc gagcgccggc
     3901 ggcacgtccg ccaactgcgc gcgccagtac gccagtccgt cctccagatt ccgctcccgc
     3961 tgccaccgtg cgaagtcgcc gtactgaatg ggaagcgtag gaagagcgga cgggcgctgc
     4021 tgcacgaacg cggcgtacag ctccgcgatc tcggccatca gcacgccgat cgaccagccg
     4081 tcggaggcga tgtggtgcat cgtcacgagc aggacgtgct catggtccgc cacgcgaatc
     4141 agtgtcacgc gaagcagcag gtcgtttgcg agatcgaatc ctcgcaccat ctcctcggcg
     4201 tacagagcgc gccaatcgga tgcttcgacg acacgaatcg gtagcgacgt ggccgcacga
     4261 acagtctgca ccggcgtccc cgacacctcg gtgatgttcg agcgaagcgt ctcgtgacgc
     4321 gcgatgatgg catcgaacgt cgcctgcaat gcatcgatcg acaacgttcc ttcgagccgc
     4381 agcgcacctg agatgttgtg cgcgacatcg gggaagagcc ggtcgcggaa ccactcccgc
     4441 tcctgcgcga atgacagcgg cacgtcgtcg ccctcggcgc gcacaaccgg cccggcaggc
     4501 cgttgctcgg tgaggcgccg cagcaacaga tcgcgctgct ccggcgagag gctcttgatc
     4561 agccccttga tcttcttggc agtcgccttc ccgtgcgcct tcgcctgctc tttgaacgtc
     4621 tctttgattc cctttccgtc actcatcgcc gtctccggaa agcccgccca cttcagcgag
     4681 catcagctcg agcagctcgg gatcggcttg ctccgcctga tgggtcacga cggcgatcgc
     4741 cagttgcgcg atggtcggcg cctgcagcaa cgccgcgagc gacatctcga cgtggagctt
     4801 caacgaaagc cgcgcgttga gctgcgtggc gagcagcgaa tgtccgccga gctcgaaaaa
     4861 gttgtcgttg acgccgaccg tcgagacgcc gaaaagctcc tgccagatcg acgcgagctc
     4921 ggactcgatc tctccgcggg gcggctcgta cgccgtccgc aaagcgggcc gctcgtgctg
     4981 aatcagcggt gccgcgtcga gctcgtcgcg gtgcccgatc caccggcgca gccgcgcctg
     5041 gaggtcgccg ctcgacacga ccacttggcc gaggccgccg gcggcgagga tgcgctcgaa
     5101 cgcgtcggcg ccttcgtgcg gctccatgac gaactcgctg actgtcgcgc cgagtccggc
     5161 gatcactggg cgcgtgtcgg ccagccgcca actgtcccaa tcgacgctcg tccagcccgt
     5221 ggcgccgtcg cgattcttcc agcgcgcgaa tgagtccatg aagagattcg cggcgctgta
     5281 cgcggagaat ccgaggccgc cgagaatcgc ggagagggac gacatcagca ggcagaaatc
     5341 cggcgtgcgt ccggccagca cctcatcgag gacgagcagc cctttgacct tggctacgaa
     5401 ctgcggctcg gagtccgcgg gcgtcgcctc gcggatctcg cggaaggtct cgatgccgac
     5461 agcgccggcc gcgtggatga cgccgtgcag ctcgccgaag cgctcgtcga ccgcggcgag
     5521 aacgtgctgc atgtcgtcgc ggctggagac gtcggccgcc aacacgagca cctcggcgcc
     5581 gaggtcctcg agctctgcga gctgccgcag cttctcatcg gtcggtgccg agcggccgat
     5641 cagaacgagc ctcgcctgaa cgcgctcggc gaggtgacgc gcgagcacca tgcccacgcc
     5701 accaagacct cccgtaatca gatagacgcc gcgctcgcga aggaccgtcg cggacgacgc
     5761 gagcttcacc ggttcgtacg cggggacgaa gcggctgccg cggcgatacg cgacgagcga
     5821 gtcgtccggc tcgctgcaga tctcgtcgag aatctgtgcg acgagcgcgt cgcggcgacc
     5881 gacgtcgaga tcggtctcga tcatgcggca gacgagattc ggatgctcct gcggcgccac
     5941 catcgccggc gcgcgcaagg tggccttctc ggggatgtcg tcgacgtgat tggtaacgat
     6001 ctcgatgttg gccgcaccct gaacaccgcg caggagctcc atgagactgt ggtagccgag
     6061 gtcctgcatt tccgcgaagc gctcggcgga aggggcggcg tcgtcgcgtg gcgtcagcga
     6121 ggcgagatga atgatgatgc gccggccgtc atcaggtacg ccgtcagcgt cgtgaccaat
     6181 ctccacgctt gcgccgcggg cgcgcaacgc gcgcgcgagc tcctcggcga tgccataacg
     6241 gtcggcgacg atgagccacg agccgctgaa gtcctcgcgg cggcgcgcgc ggaccgacgg
     6301 cttccacgac ggcagataga accactccgc gacgtcgtct tttctgtgcg cggatggcgg
     6361 cgcgtccgcg gcgggcgggt cgatccagta gcgccggcgc tcgaatggat acgacggcag
     6421 ccgcacacgg cgtgggtcag gagtggtcag gaagcgcgcg gtgtcgagcg atgcgcccgc
     6481 gagccacaac tgtcccaccc cgcgcagaag gacttcgagc tccgactgca agccgtacga
     6541 ccagcgcatc gacgggatga ttgcgttctc gcgcttctgg gcgatggcgt gcgaagtgag
     6601 gccctggccg gggccggcct cgaacaggac gcggcgcgga tcgtcgagca gcgtcgaaag
     6661 tccgtccgcg aagcgcacgg tgcggcagag atgccgcgcc caatacccgg gatcagtcgc
     6721 ctcggcgtca gtaatccacg tcccggtgat gcacgagacg tacggtatcc gcggcgcatg
     6781 caatgcgacg cgccgcagca cggcgaggag cggctcgacg atcgcctcca tcattgaaga
     6841 gtgataggcg tgcgtgctcc gcagccgttg gcaactgatg tcgcgcccgc cgagcgttgc
     6901 cgccagttcc gcaatgcccg actcatcgcc cgacgcgatg cagagccgcg gggcgttgat
     6961 cgcgccgagg gacacgccgc taggcaacaa cggcagcagc tcggcctcgg tgagcggaac
     7021 cgccagcatc gcgccgggag tgacgtgcga ctgaatcagg cgcgcgcgtg cagcgacgac
     7081 ttcgacggca tcggccagcg acagaacgcc gctcaggtgc gctgccgtga actcgccgat
     7141 gctgtggccg atcatcgcct ccggctcgag gccccagctc cgcaacaact cggcgagcgc
     7201 gacctctgta acgaagatcg ccggctgcgc ggcgaccggc tgatcgtccg gcgcgacgcg
     7261 ctttttgccg agcatggcgc ggaggtccag actgcctttg cttttcgtgg gcggctcgag
     7321 ccgtggatgc gaccagtggc ggtcgggata gaggaagtcg cggatgtcgc cggcaaggtg
     7381 attcttcagc aatgctgcgg agcgatcgac gctgtcgcgg aacaccggtt ctgtgcagta
     7441 caactcccaa cccatgccgg ggtagtgatc gccgaggccg gggaacatga atgcgatcga
     7501 gggacggccg gcgggcgcgg agccggtgac gatgcgctgc gggtcgcgca acgcggcaac
     7561 cgcatcgtcg ggatccatgg ccacggcgaa gcggcgctcg ctgaacgcgc ggcgtccgtg
     7621 ccgcagcgtg tacgcgacgt cggaaagacg cgcagcctga tcggcctgga gatgatcggc
     7681 gaggcggttg gtggcggcgt cgagcgcggt cggggttctg gccgaaagca aaagcagatc
     7741 gaacggccgc gcctcgggtg acggttcgga gacgacgggc gcctcctcca gca
//
LOCUS       DATLVW010000273         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_218281, whole genome shotgun sequence.
ACCESSION   DATLVW010000273 DATLVW010000000
VERSION     DATLVW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218281"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VNN25_08510"
     CDS             <1..1078
                     /locus_tag="VNN25_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH91608.1"
                     /translation="FNPRPAWTYPDSLMGVISYIKQTLLDAQQYSTARTIYDKNPAGY
                     KRPDESPSLEALGAVLRRDVPVVFVADSELMIRRVEAIAKEFNIRPVIAGARQGYKMG
                     DELKSLGAPVLVSIKWPVAPTSKEDREEQPLRVIRDRQLAPTTPAVLAKSGVLFALVS
                     GAGKTADFVPGIRKAIENGLSADDALRATTINPAKIIGIDRQLGSLDRGKIANIVVTD
                     KPIFDKEAKVKRIFIDGRESKVPAEDEKKKPGGAGTSAGTSASALDGSWSFVVKTTAG
                     DASISATLHVENGQISGTFSGDRGSGSIGNGTFDGTTVEFSIAGRGKEEAGDWLFHGT
                     LHGSNMEGTVATTLGTFPFTGSKK"
     gene            1204..>2112
                     /locus_tag="VNN25_08515"
     CDS             1204..>2112
                     /locus_tag="VNN25_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXH91609.1"
                     /translation="MLATAFLLAATTLTAQDIVIRDATILTITHGKIEHGSILIHGGK
                     IAAVGANDRVNAPANATVIDAAGKFVMPGIVDTHSHTAVEGSVNEISLPNSGMVRIGD
                     VLNNEDIDVYRQLAGGTTTALVLHGSANPIGGQSQIVKWKWGHPVSEWPISDAPRTIK
                     FALGENPKSSNFRPPPGIPAQYPQTRMGVEEVIRQSFTDAKDYLARWDDYDARKKRGE
                     NPIPPRRDLLMETMADILRGKIDVHSHCYRADEIMMLLNLSDEQGFKIRELQHVLEGY
                     KVAKEIAAHGVGGGTFIDWWGFKAEAQ"
BASE COUNT          443 a          670 c          669 g          330 t
ORIGIN      
        1 gttcaatccg cggccggcct ggacctatcc cgactcgctg atgggcgtca tctcgtacat
       61 caaacagacg ctgctcgatg cgcagcagta cagcacggca cggacgatct acgacaagaa
      121 tcctgccggc tacaagcgcc ccgacgagag cccttcgctc gaagcgctcg gcgcggttct
      181 tcgccgcgac gtcccggtcg tcttcgtcgc cgacagcgag ttgatgatcc gccgcgttga
      241 agcgatcgcg aaagagttca acatccggcc ggtcattgcc ggcgcgcgcc agggctacaa
      301 gatgggcgac gagctgaagt cgctcggcgc gccggtgctc gtctcgatca agtggccggt
      361 cgcgccgacg tcgaaagagg accgcgaaga acagccgttg cgagtgattc gcgaccgcca
      421 gctcgcgccg accacgcccg cggttctggc aaagagcggc gtgctcttcg cgctcgtcag
      481 tggggcagga aagaccgccg acttcgtgcc cggcattcgc aaggcgatcg agaacggcct
      541 ctccgccgac gacgctctgc gtgctacgac gatcaatccg gccaagatca tcggcatcga
      601 ccgccagctc ggatcgctcg accgcggcaa gatcgccaac atcgtcgtca ccgacaaacc
      661 gatcttcgac aaggaagcga aggtgaagcg gatcttcatc gacgggcgcg aatcaaaagt
      721 gcctgccgag gatgagaaga agaagcctgg cggcgcgggt acgtcggcgg gaacgagcgc
      781 atcggctctc gacggcagtt ggagtttcgt cgtcaagacc actgccggcg acgccagcat
      841 ctccgcgacg ctgcacgtcg agaacggcca gatcagcggc acattctccg gcgaccgcgg
      901 ctccggcagc atcggcaatg gaacgttcga cggcacgacg gtcgagttct cgatcgccgg
      961 ccgtggcaag gaagaagccg gcgactggct gttccacggc acgctgcacg gctcgaacat
     1021 ggaagggacc gtggcgacaa cgctcggcac gttcccgttc acggggagca agaaatgaga
     1081 atcttaaaag acgttgtcgg gagaagcaga aaagacgttg tcggttgtcg gttgtcagtg
     1141 gccggcgatc agccagccag ctccgacacc cgacccccga ctcccaatat ccgattcaag
     1201 accttgctcg cgaccgcatt cctgctcgcg gcaacaacac tcaccgccca ggacatcgtc
     1261 atccgcgatg ccacgatcct gacgatcacg catggcaaga tcgagcatgg atcgatcttg
     1321 attcacggcg gaaagatcgc ggccgttggt gcgaacgatc gcgtcaacgc accggcgaat
     1381 gcgacggtca tcgatgcggc gggcaagttc gtgatgcccg gcatcgtcga cacgcacagc
     1441 cacaccgcgg tcgaaggaag cgtcaacgaa atctcgctgc cgaacagcgg catggtgcgt
     1501 atcggcgacg tgctgaacaa cgaagacatc gacgtctatc gccagcttgc cggcgggacg
     1561 acgaccgcgc tcgtgcttca cggctcggcg aatccgatcg gcggccagtc gcagatcgtg
     1621 aagtggaagt ggggacatcc ggtctccgag tggccgatct ccgacgcgcc gcgcacgatc
     1681 aaattcgcgc tcggcgagaa tccgaagtca tcgaacttcc gcccgcctcc gggtatcccc
     1741 gcgcagtatc cgcagacgcg catgggcgtc gaggaagtga tccggcagtc gttcacggac
     1801 gcgaaggact atctcgctcg ttgggacgac tacgacgccc gcaagaagcg cggcgagaac
     1861 ccgattccgc cgcggcgcga tctgctgatg gagacgatgg ccgacatcct ccgcggcaag
     1921 atcgacgtgc actcgcactg ctaccgtgcc gacgagatca tgatgctgct caatctctcc
     1981 gacgagcagg gcttcaagat tcgcgagctg cagcacgtgc tggaaggcta caaagtggcg
     2041 aaggagatcg ccgcgcatgg cgtgggcggc ggcacgttca tcgactggtg gggattcaaa
     2101 gcggaggcgc ag
//
LOCUS       DATLVW010000274         3150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_381372, whole genome shotgun sequence.
ACCESSION   DATLVW010000274 DATLVW010000000
VERSION     DATLVW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381372"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1301)
                     /locus_tag="VNN25_08520"
     CDS             complement(45..1301)
                     /locus_tag="VNN25_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXH91610.1"
                     /translation="MLAAQSTISTITPSSGLTRGGVLVDIRGTNLLAAECPGPNCKTF
                     VTFGGSFATIVFNTADEIVVVAPAHASGAVDVVISIGGALPVVVTCGFTFQTPAEGEN
                     VRFLLPISGGITGVLNTTWRTDVSVTNENAIPVTIAGTAVPPLTTKTLLLSGTSAFVD
                     IPRELSDGVTISVRVQDTTHDADSLGVDVPAVPPSQFRKSVVLTSLPSDPRYRMLLRI
                     YGYGGPGSAIVRIRDANTGALLDQMTADLTGSSPSYAQIALSASASVPRTVEVTTAGR
                     SDPPIWAFVSVTNNITQQVTLATPRVGVAGSASSDAPLLATGHWGGNGCMEVSEQDVF
                     VGATCSFGSFPRPVLEADGHFEADGKWFGPSVGPLPPLPISDPAHFSGMVQGSKLALV
                     VRTGSRVLGPFLVELGSSIPCSPACP"
     gene            1493..2755
                     /locus_tag="VNN25_08525"
     CDS             1493..2755
                     /locus_tag="VNN25_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91611.1"
                     /translation="MRVSQRIVIALVALAPLSVFAAAPPPNCAQPPLCTGSTCQTSIS
                     KGSATVPDKPCPRFGGNQDDVDIFSWNEFIAYNWPASKTCSADLTRSIAQVKSGANGP
                     LVWQAQMSTDDLFVASGRPKGWCGTPTLFTTAPRSFRHIAKADPAARLLGADFAKVAE
                     PEGPVQAGGGILTDASGRWVRYEKLVDLTEYKYILTNNLWNKAGLAGKTVLFPSGSTE
                     FKASWKVLTPAEIAGGRYYTTSATVYNTPDGSQPSPLPNPVTLGLVGLHIVHKGASGF
                     FWSTFEQVDNEKVFVDPRNPAGPNVQTAKTPYREIDPVTKKPVDLGVNVKRLTAIPAS
                     SAVNDFYRGLLAGSVFANYRLISTQWQFNLNTGGAPQHVANITMETYVQLKSKPDPKK
                     PTNQPFTGCLACHGFSPWDQSFVFIEAK"
     gene            complement(2822..>3150)
                     /locus_tag="VNN25_08530"
     CDS             complement(2822..>3150)
                     /locus_tag="VNN25_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91612.1"
                     /translation="EYGLRDYTVRDLYGYELRFGHYIYNMGPAIEIERVDVSVRLEKR
                     LAALLQDLAEHNHVSLTSLFEETFLHTLDGVGPHGDRTLKYIQKLKEKHAIDYDSHGS
                     YRFVER"
BASE COUNT          676 a          994 c          912 g          568 t
ORIGIN      
        1 ggtccgcccg tttttcgtgc gcagcgtcat accgatttgc agtgctacgg acatgccggc
       61 gaacaaggta tcgacgaacc caattcgacg aggaatgggc ctaatacgcg ggagcccgtc
      121 cgcaccacaa gcgccagttt cgagccttgg accatccctg agaaatgcgc tggatcgctg
      181 atcggtagcg gcggaagcgg accgaccgag ggcccgaacc atttaccgtc ggcctcaaag
      241 tggccatcgg cttcgagcac cggccgcggg aagctcccga atgaacaggt ggccccgaca
      301 aatacatcct gctctgacac ctccatacaa ccattcccac cccagtgacc ggtcgcaaga
      361 agaggtgcgt cggaggatgc gctgcccgca acaccaacgc gcggcgtggc gagcgtcacc
      421 tgctgggtga tgttgtttgt gacgctcaca aaggcccaga tgggcggatc ggatcggccc
      481 gccgtcgtga cctccaccgt ccgcggcacg gaagcggagg cgctgagggc gatctgcgcg
      541 tacgaaggcg acgatccggt caagtcggcg gtcatctggt cgagcaacgc acccgtattg
      601 gcgtcgcgaa tgcgaacgat ggccgatccc gggccaccat agccgtaaat cctgaggagc
      661 attcggtagc gcggatcgga aggcaaactg gtaaggacca cggacttccg aaactgcgaa
      721 ggaggaaccg ccgggacatc gacgccgagg ctgtccgcat catgcgtcgt gtcctgtacg
      781 cggaccgaga tcgtcacgcc gtccgagagc tcccgtggga tgtcgacaaa cgccgacgtg
      841 cctgacaaga gaagcgtttt cgtcgtgagc ggcggaacgg ccgtaccggc aatcgttacg
      901 gggatcgcat tctcgttcgt caccgaaaca tcggtacgcc aggtcgtatt gaggacgccc
      961 gtgatgcctc cagagattgg aagcagaaag cggacattct cgccttcggc aggtgtttgg
     1021 aatgtgaagc cgcaggtcac gacgaccggc aatgcgccgc cgatcgaaat cacgacgtcg
     1081 accgcgccgg aagcatgcgc cggtgcgacg acgacgatct cgtcggcggt gttgaatacg
     1141 atcgtcgcga aactgccgcc gaacgtcacg aaagtcttgc agttcggacc gggacactcc
     1201 gcagcgagca agttggtacc gcgtatgtcg acgagcacgc cgccgcgcgt gagtccggaa
     1261 gaaggagtga ttgtcgaaat ggtgctttgt gccgccaaca ccgtcgcaat gaagcatccg
     1321 atgaaagtcc aaacacaaat ccgaagcatg agcgaaaccc ccagatggtg tcgctccaga
     1381 ttacttcagt tccgtcagaa caagcgtcgt cgccgggctc atacattcac gaaggtcaga
     1441 cgtgtggctt ccatttcatt gtctaatctc ggaatgttcg aggaggacaa acttgagagt
     1501 tagccaacgc atcgtcatcg ccctggtcgc actcgctccc ctttccgtct tcgccgccgc
     1561 accgcccccg aattgtgctc agccgccgtt atgtacaggc tcaacctgcc agaccagcat
     1621 cagtaaagga agtgccaccg tccccgacaa accatgtccc cgcttcggtg gtaaccagga
     1681 cgatgtcgac atcttctcct ggaacgagtt catcgcgtac aactggcccg cctcgaagac
     1741 gtgctccgcc gatctgacgc gatcgatcgc gcaggtcaag agcggcgcca atggtccgct
     1801 cgtttggcag gcgcagatgt cgaccgacga cctcttcgta gcttcgggcc gcccgaaagg
     1861 atggtgcggc acgccgaccc tcttcacaac ggcgccgcgc tccttccgcc acatcgcgaa
     1921 agcggatccc gcggccaggc ttctcggcgc cgacttcgcc aaagtcgcgg agccggaagg
     1981 tcctgtgcag gcaggtggcg gcattctgac cgatgcatcc ggacgctggg tgcgttacga
     2041 gaaactcgtc gacctaaccg agtacaagta catcctcacc aacaatctct ggaacaaggc
     2101 cggactcgcc ggcaagaccg tgctgttccc ttccggatca accgaattca aagcgtcgtg
     2161 gaaagttctc acgcccgctg agatcgcggg cggccggtac tacacgacat cggcaacggt
     2221 ctacaacacg ccggatggca gccaaccgag tccgcttccg aacccggtga cgctcggcct
     2281 cgtcggcctg cacatcgttc ataaaggcgc gtcgggattc ttctggtcca cgttcgagca
     2341 ggtcgacaac gagaaggtct tcgtcgatcc gcgaaatcca gccggaccga acgtccagac
     2401 cgcaaagaca ccgtatcgcg agatcgatcc ggtcacgaag aagccggtcg acctcggcgt
     2461 caacgtgaag cggctcacgg caatcccagc cagctccgcg gtgaacgact tctaccgcgg
     2521 cctgctcgcc ggctcggtct tcgcgaacta tcgcctcatc agcactcagt ggcagttcaa
     2581 tctcaacacc ggcggcgctc ctcagcacgt cgcgaacatc acgatggaaa cctacgtgca
     2641 gctcaagagc aaaccggatc cgaagaaacc aaccaatcag ccgttcaccg gctgccttgc
     2701 ctgccacggg ttctcgccgt gggatcagag cttcgtgttc atcgaagcga agtaaaactg
     2761 cgaaatcgac ctgaatgcgg cggctcattc gaatccggat gagccgctct cttcattcca
     2821 cctaccgttc cacgaaccga taactcccgt gactgtcgta gtcgatggcg tgcttttcct
     2881 tcagcttctg gatgtatttg agcgtgcggt ctccgtgcgg gccgactccg tcgagcgtgt
     2941 gcaggaacgt ctcttcgaac agactcgtca ggctcacgtg gttgtgctcc gccagatctt
     3001 gaagcaacgc ggccagccgc ttctctaagc gcacggaaac atcgacgcgc tcgatctcga
     3061 tcgcgggacc catgttgtag atgtaatgtc cgaagcgaag ctcgtagccg taaagatcgc
     3121 gaaccgtgta gtcgcgaagc ccgtattcgc
//
LOCUS       DATLVW010000275         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_218524, whole genome shotgun sequence.
ACCESSION   DATLVW010000275 DATLVW010000000
VERSION     DATLVW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218524"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="VNN25_08535"
     CDS             complement(<1..1905)
                     /locus_tag="VNN25_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH91613.1"
                     /translation="MPLKPDRRWEAFAAREPYYAVFTDPRFRTANRTPHSEREFFDSG
                     ELLVDLLFHTIKRKLAPDFNPELVLEYGCGPGRLAIPFARRVGSVTAVDRSPAMLDVA
                     KREAAKQTVVNIAFKPASDFFSEPRKFDLVNCYHVFQRLRPAAGLALFRKLLGCLGPG
                     GVGAFHFLYRSTTPSIVEWTRLARERLPAVNAVANLARHKALDEPFIPSHTTDIDDVF
                     RILDDDGFLTSHVVFERNAEVTGAIVLVQAPLSFTSIESDFTPSITATPDDAIINVRD
                     LIARTSIDDLNRTAEEYFASLTQWEHHLAKPFSKADETPALLLNVSMLLQGLRLTPGT
                     TVLEFGAGTGWLSRFLTQLGCRVILLDVSATALKIARDLYERQPPIGERPKPQFLEFD
                     GRRINLPDASVERIICFDAFHHSPNPDAMLREFGRVLKPGGIAAFAEPGPRHSRSPLS
                     QFEMRTYGVIENDVDIQSIWRTAQTSGFRDLKMIVFHGTPFHISLDEYEDLLAGGAPC
                     ATWMASTRAFLRNVRSFYLYREGFERLDSRSANGLVCQIEATHTGAPAKDGQPVLIDV
                     TVTNRGTATWLASNDPVGGVALGVHLYDTAGKLLKLDLHWQDLTDPPREIAPEETVHV
                     RMALPRLSAGN"
     gene            1932..>2066
                     /locus_tag="VNN25_08540"
     CDS             1932..>2066
                     /locus_tag="VNN25_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXH91614.1"
                     /translation="MNLILLFDDDFITATRVKLSDRRLEHVTNVHRAAVGDALVVGVA
                     N"
BASE COUNT          396 a          553 c          729 g          388 t
ORIGIN      
        1 gttgccggcg ctcaaacgag gtaaggccat gcgaacgtgt acggtctctt caggtgcgat
       61 ctcgcgggga gggtcggtca ggtcttgcca gtgaaggtcg agcttcagaa gttttccagc
      121 cgtgtcgtaa agatgaacgc cgagggcgac tccgccgacg ggatcattgg aggcaagcca
      181 ggtggcggtg ccgcggttcg tgacggtgac gtcgatcagc accggctggc cgtccttcgc
      241 cggggctccg gtgtgcgtcg cttcgatctg gcaaacgagg ccgttcgcgc tgcggctgtc
      301 gagacgttcg aaaccttcgc gatagagata gaagctgcgg acattgcgca ggaaggcgcg
      361 ggttgacgcc atccaggtcg cgcaaggtgc accgccggca aggaggtctt cgtactcatc
      421 gagcgagatg tggaacggcg ttccgtgaaa gacgatcatc ttcagatcgc gaaaacccga
      481 agtctgggcg gtgcgccaga tcgactggat gtcgacgtcg ttctcgatga ctccgtacgt
      541 tcgcatctcg aattgcgaga ggggagagcg agagtggcgc ggcccgggtt ctgcgaaggc
      601 ggcgatcccg cccggcttga gcacgcgtcc gaactcgcgc agcatcgcgt cgggattggg
      661 cgagtggtgg aaggcatcga aacagatgat ccgctcgacg ctggcgtcgg gaagattgat
      721 gcgccgcccg tcgaactcta ggaattgcgg ctttgggcgc tctccgatgg gcggctggcg
      781 ctcgtacaga tcgcgcgcga tcttcagcgc cgtggcggag acgtcgagga ggatcacgcg
      841 gcagccgagc tgcgtgagga accgcgagag ccatccggtg ccggcgccga actccagcac
      901 cgtggttcct ggagtgagcc gcagaccctg cagcagcatc gacacattca aaagaagtgc
      961 gggcgtctcg tcggccttgc tgaatggttt ggcgagatgg tgttcccact gcgtgagcga
     1021 tgcgaagtac tcctcggcag tccgattgag atcatcgatc gatgttcgcg caatcaggtc
     1081 tcgaacgttg atgatggcgt catccggcgt tgcagtgatc gaaggtgtga agtcgctttc
     1141 gatggaggtg aatgaaagcg gcgcctgcac gaggacgata gcgcccgtca cctcagcgtt
     1201 tcgttcgaag acgacgtggc tggtgaggaa gccatcgtca tcgagaatgc gaaagacgtc
     1261 gtcgatgtcg gttgtgtggc tggggataaa cggctcgtcg agcgctttgt gacgcgcaag
     1321 attggcgact gcattgacgg cgggcagccg ctcgcgtgcg aggcgggtcc attcgacgat
     1381 agagggtgtc gtgctgcgat agaggaagtg aaacgcaccg actccgccgg ggccgaggca
     1441 gccgaggagt ttgcgaaaga gggcgagtcc ggctgcgggg cgaaggcgct ggaagacgtg
     1501 atagcaattg accagatcga acttgcgcgg ctcggagaag aagtcgctgg ctggcttgaa
     1561 agcgatattg acgacggtct gcttcgcagc ctcgcgcttt gccacgtcga gcatggccgg
     1621 cgagcggtct accgcggtga cggatccaac gcggcgggcg aagggaatgg ccagccgtcc
     1681 ggggccgcaa ccgtactcga ggaccaactc ggggttgaag tcgggtgcaa gctttcgttt
     1741 gatcgtgtgg aaaagcaagt cgacgagtag ctcgccgctg tcgaagaact cccgctcgct
     1801 gtgcggcgtt cggttcgcgg ttcggaatct cggatcagtg aagaccgcgt agtacggctc
     1861 gcgcgcagcg aacgcttccc atcgacggtc tggcttcaac ggcatgcgaa gggattatcg
     1921 ttgcaaccgc cgtgaacctg atcctcctat ttgacgacga cttcatcacc gcgacgcggg
     1981 tgaagctttc cgatcgccgc ctcgagcatg tgacgaacgt gcatagagct gcggtggggg
     2041 atgcactggt ggtcggagtg gccaac
//
LOCUS       DATLVW010000276         6092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_10948, whole genome shotgun sequence.
ACCESSION   DATLVW010000276 DATLVW010000000
VERSION     DATLVW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10948"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..816)
                     /locus_tag="VNN25_08545"
     CDS             complement(223..816)
                     /locus_tag="VNN25_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXH91615.1"
                     /translation="MNVVEHFLIGWCVANISPELTNRERIIITAAAVIPDIDGLGLLI
                     EIPTRNTAHPILWWTEYHHVLAHNVGFAALVTIVAFALATHRWQTTLLTILSFHTHIL
                     GDILGARGPDGYQWPIPYFLPFSSTPVLSWDGQWALNGWQNFVITAAALAVTFVLAWR
                     RGYSPVGIFSQQADERFVQALRSRVSVSSPSSQRRGP"
     gene            complement(827..1303)
                     /locus_tag="VNN25_08550"
     CDS             complement(827..1303)
                     /locus_tag="VNN25_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91616.1"
                     /translation="MRIVNVTAAIFRAVPILAQYSDDDSGLVRIGLLRAGLSPEQTTE
                     AIAFIPLAFGRELLSGMGVTLSETYMQASDGGSGVERRLADEPMFSEARKLVPRIAAE
                     MGQGVFTAVALQSSELKAVNEALNQGADPVNLVASPPVVLLPHAPARDKRPWWKFW"
     gene            complement(1362..1853)
                     /locus_tag="VNN25_08555"
     CDS             complement(1362..1853)
                     /locus_tag="VNN25_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91617.1"
                     /translation="MNCVLWLIFSLLAVSSDAVSHRFVFTQFRAEPRLHQPPAPPRLS
                     DPRARRYRTVLRDAAKEGPNFNGHYRVVSWGCGTNCIEWAVIDQEGGDVWMAPDPLAS
                     CWFPDAPDAKVPDWFSFRINSSLLIQHECRSPSNRQTFDTTNYFAWKHGHPELLGTEA
                     LRY"
     gene            1899..2330
                     /locus_tag="VNN25_08560"
     CDS             1899..2330
                     /locus_tag="VNN25_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91618.1"
                     /translation="MPELMGLFQVDDAGLLFISPVIDDWASIEAHGIDVVIDMEGGVD
                     DGVPTTPNASLYVYFPIFDEELPDLARLEAVAIMGAHLVSSGHRVLSHCGMGFNRSAL
                     IAARILHQLGMQGPAIVEQLRARRPGALFNEVFAQHVASLT"
     gene            2336..2950
                     /locus_tag="VNN25_08565"
     CDS             2336..2950
                     /locus_tag="VNN25_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91619.1"
                     /translation="MDWTAKEKRILNGLRTPAHIQHYLDELDYDDVGGADSPRVVMRQ
                     EKAQCFSGVLFACAALRELGHPPRLMYMDAASDDGHCLAVYEIGGLWGAIGKSNFTTL
                     RSREAIYPYLALGLSYFEGFFNVYGKRTMRNFTVPVELERFEPRGWRFSEKELLYIDR
                     AIDLAPKAWVLPRRTISGISKVSEQLRTAGLLGSKASGLWRRNG"
     gene            3047..3511
                     /locus_tag="VNN25_08570"
     CDS             3047..3511
                     /locus_tag="VNN25_08570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXH91620.1"
                     /translation="MTSVLQPPRIKPKSFTYHTGVADVEGRSAVLSSDGKPPLRVASP
                     PEFKGVANVWTPEDFFVAAIEVCLMLTFVGLAEKHGLKFASYESKAEGLLEWDQQSYG
                     FTRVVVRPAITLFDEHSIVAAHETLERAHATCLVAKSVACDVVVEPIFAVKR"
     gene            3508..5415
                     /locus_tag="VNN25_08575"
     CDS             3508..5415
                     /locus_tag="VNN25_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXH91621.1"
                     /translation="MSVQAVGAQPAIVAVDDNPGGRKRLSKETWIALASLLGISTSLI
                     LRFVVRTPARVSDWVLIAVLLAGLPLLFDLLKKLAAFEVGSDALAGISILTAAILGEY
                     LAGAIIVLMLSGGTALELYATRRASTVLSALAKRVPSSAHRLVESGIAEVSMADVRID
                     DRLVVLPHEVCPADGIVVEGHGAMDESYLTGEPFQIAKAPGSEVLSGAINGADAMTIR
                     VTTLPIDSRYAKIVQVMEQAEAQRPRLRRIADRLGGWYTLFALVIAFAGWIIGHDPRR
                     FLAVLVIATPCPLLLAIPVAIIGAISLAASRGIIIKNPSMLERIDRCRTIIFDKTGTL
                     TYGRPSLTDVICAPGVTREQALRLATSLEQYSRHPLAISILEAARAEGIAAVNVSEIS
                     ERPGEGLRGRIDGDSVEITGRAKVTSNRVIAQLPPHAAGMECLVLRNGEYAATLRFQD
                     VPREESRSFVDHLMPHHQVTRVMLVSGDRDTEVQHLATLVGIENVHSGTSPEEKLAIV
                     RDEVAKGPTLFLGDGINDAPAMEAATVSVAFGHNSDITAEAADAVVLEPSLAAVDELI
                     HIGRRMRRIALQSAAGGMTLSMIGMLAAAAGWLAPVPGAIAQEVIDVLAVLNALRVAA
                     PRMRLNDLGHD"
     gene            complement(5495..5776)
                     /locus_tag="VNN25_08580"
     CDS             complement(5495..5776)
                     /locus_tag="VNN25_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HXH91622.1"
                     /translation="MAVLTGMLIVPISEARTRIYLRIGPPPLVVEQRPVLVRPGYVWQ
                     QGYHRWDGNRYVWTSGAWARAPYRSAVWVPAHWVHTRRGYYFVDGHWAR"
     gene            complement(5890..>6092)
                     /locus_tag="VNN25_08585"
     CDS             complement(5890..>6092)
                     /locus_tag="VNN25_08585"
                     /inference="COORDINATES: protein motif:HMM:NF017457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agenet domain-containing protein"
                     /protein_id="HXH91623.1"
                     /translation="QTRSDAAIGRRVEVRSDGDWYKARIIDAKNHRYRVHYFGYEVAD
                     DEWVGPDALRRTGQRPRAGRAR"
BASE COUNT         1140 a         1944 c         1866 g         1142 t
ORIGIN      
        1 tcggaatacg tcatacgagg cggcaccaat gcgttgatcg tacaggttgc gacgaccgtc
       61 cagtactgta cgcctccagc gccataacga gatcaacgac gagatcggcg cgttacgaat
      121 cgcttttgcg actcgcaggt aacgcattgc ctattgggac tcgtccgttc aagtccaagg
      181 cggcgccgtc ctccgatagc cagtcgccga agagtattct cgttacggtc ctcttcgctg
      241 cgacgaaggc gagctaacgc tgactcgcga tctcagcgct tgcacgaaac gctcgtcggc
      301 ttgctgcgaa aagattccga ctggcgagta tcctctgcgc caggccagca caaaggtcac
      361 ggcaagcgcc gcagccgtga tcacgaagtt ctgccagcca ttgagcgccc actggccgtc
      421 ccatgaaagg acaggcgtgg acgagaacgg caggaaatag gggatcggcc actgatagcc
      481 gtccgggccg cgcgcgccga ggatgtcgcc gaggatgtgg gtgtggaagc ttaggatcgt
      541 cagaagggtg gtctgccagc gatgcgtcgc cagcgcaaat gccacgatcg tgacgagagc
      601 cgcgaatcca acattgtggg cgaggacgtg gtgatactcc gtccaccaca agatcggatg
      661 agcggtgtta cgggttggga tctcaatcag gaggcccagt ccgtcgatat caggaatcac
      721 ggccgcagcc gtgatgatga tccgttcgcg attggtcaac tccggcgaga tgttggccac
      781 gcaccagccg atcaggaaat gttcgacgac gttcatggcc agcgaattac cagaacttcc
      841 accagggacg tttatcgcga gccggagcgt gcggcaaaag aacaaccgga ggcgacgcca
      901 caaggttgac cggatccgct ccctggttga gcgcctcgtt gacggccttg agctcgctgc
      961 tctgtagagc cacggcggta aacacgccct gccccatctc cgcggcaatc ctcggcacaa
     1021 gtttccgcgc ctcggagaac atcggctcgt ccgcgagtct gcgttcgacg ccactgccac
     1081 cgtcgctggc ctgcatatag gtctccgaca gcgtgactcc catcccgctc aacaactccc
     1141 gcccgaatgc cagcggtatg aatgcgatcg cttcggtcgt ctgctccgga gacaatccgg
     1201 ctcgcagcaa tccgattcgc acaaggccgg agtcgtcgtc gctgtactga gcgaggattg
     1261 gtaccgcacg aaaaatcgct gctgttacgt tcacgattct cacttcggca ttgagatcgc
     1321 tgctcgatcg tttgacgggg aacgagtggg aaaggtgcag tttagtaccg cagcgcctcg
     1381 gtgccgagga gctcgggatg gccgtgcttc caggcaaagt agttcgtcgt gtcgaacgtc
     1441 tgccggttcg acggcgaccg gcattcgtgc tggatcagca ggctgctgtt gatgcggaac
     1501 gagaaccaat cgggaacctt cgcgtcgggc gcatcgggaa accagcagga ggcaagcgga
     1561 tcgggcgcca tccagacatc gccgccctcc tgatcgatca ccgcccactc gatgcagttc
     1621 gtgccgcatc cccacgagac gacgcgatag tggccgttga agttaggccc ttccttcgcg
     1681 gcgtcgcgca ggaccgtgcg ataacggcgc gcccgaggat cggacagacg tggcggcgcg
     1741 ggaggctgat ggagccgcgg ttccgcgcgg aattgcgtga atacgaaccg gtgcgaaacg
     1801 gcgtccgacg aaacggccaa cagcgagaag atgagccaga gcacgcagtt catcaccttt
     1861 cgacgaggcc agccgcgatt atcctaccct gcgttaccat gccggagctt atgggcctct
     1921 ttcaagtcga cgacgcaggt ctgctcttca tctcccccgt catcgacgac tgggcgtcca
     1981 tcgaggccca cggcatcgat gtcgtgatcg acatggaagg cggagtggac gatggcgtcc
     2041 cgacgacacc gaacgcttcc ctttacgtct acttcccgat cttcgacgaa gagcttcccg
     2101 acctggcgcg cctcgaggct gtggcgatca tgggcgcgca cctcgtaagc agcggccatc
     2161 gcgtcctctc tcattgtggg atgggattca accgctcggc gctgatcgct gcccggatcc
     2221 tgcatcagct cgggatgcaa gggcccgcga tcgtcgagca gcttcgcgca cgaaggccgg
     2281 gcgcgctgtt caatgaagtg tttgcgcagc atgtcgcttc attaacatag agaatatgga
     2341 ctggaccgca aaagagaagc gcattctgaa cggactgcgc acccccgccc acatacaaca
     2401 ctacctcgat gagctcgact atgacgacgt gggaggtgcg gactcgccgc gcgtcgtcat
     2461 gcggcaagaa aaagcgcaat gtttcagcgg cgtccttttc gcctgcgcag cgctgcgcga
     2521 gcttggccat cctccacgtc tgatgtacat ggacgcggca agcgatgacg gccactgcct
     2581 cgcggtgtat gaaatcggag gactgtgggg cgcgatcggg aaatcgaact tcacgacgct
     2641 ccgttcccgc gaggccatct atccctatct cgccctcggc ctcagctact tcgaaggctt
     2701 cttcaacgtc tacggcaaac gaacgatgcg caactttacg gttcccgtcg agctggagcg
     2761 cttcgagccc cgcggatggc ggttcagcga aaaagagctt ctttacatcg atcgcgcaat
     2821 cgaccttgct ccgaaagcgt gggttcttcc tcgccgcacc atctccggta tcagcaaagt
     2881 gagcgagcag cttcgcaccg ccggactcct cggatcgaag gcctcgggtt tgtggcgccg
     2941 caacggctga gcttaaacgt caccctgtga cgcgcggcac attctcctgt gtcatttacc
     3001 gctgcatcct tccgctatgg gcgacatcgt tagcgtgagg tgatccatga ccagcgtgct
     3061 gcagccgccg aggattaagc caaaatcgtt tacgtatcac acgggagtgg ccgacgttga
     3121 agggcgctcc gcagtcttga gctctgatgg caaaccgccg ttgcgcgtgg ccagtccgcc
     3181 ggagttcaaa ggcgtggcga acgtctggac ccccgaggac ttcttcgtcg ccgccatcga
     3241 agtgtgcctg atgctcacgt tcgtcggcct cgcggagaag cacggcctca agttcgcgag
     3301 ctacgagagc aaggccgaag gtctgctcga atgggatcag cagtcgtacg ggttcacgcg
     3361 cgtggtcgtg cggcctgcga tcacgttgtt cgatgagcac tcgatcgtcg ccgcgcacga
     3421 gactctcgaa cgcgcgcacg ccacgtgcct cgtcgcgaag tcggtcgcgt gtgatgtcgt
     3481 ggtcgagccg atcttcgctg tcaagcgatg agcgttcaag ccgtcggcgc gcagccagcg
     3541 atcgttgcgg tcgacgacaa tccaggcggg cgcaagaggc tttcgaagga gacctggatc
     3601 gctcttgcct cgctcctcgg tatcagtact tctctcatcc tccgcttcgt cgttcgcacg
     3661 ccggcgcggg tcagcgactg ggtgctcatc gccgtgctcc tcgccggcct gccgctcctc
     3721 ttcgatctgc tgaagaagct cgccgcattc gaagtcgggt ccgatgctct cgccggcatc
     3781 tccatcctca ccgccgcaat cctcggcgaa tacctcgccg gcgccatcat cgttctgatg
     3841 ctctcgggcg gcaccgcgct ggagctttat gcaacgcgcc gcgcatctac ggttctctcc
     3901 gcgctcgcga agcgcgttcc gagcagcgcg caccggctgg tggaatcggg cattgccgag
     3961 gtcagcatgg ctgatgtgcg aatcgatgac cgcctcgtcg tactgcctca cgaggtctgt
     4021 cctgctgacg gaatcgtcgt cgaaggacac ggcgcgatgg atgagtcgta cctcacgggc
     4081 gagccgtttc agatcgcgaa agcgcccgga tcggaagtcc tctccggcgc gatcaacggc
     4141 gcagacgcga tgacgatccg ggtcaccacg ctgccaatcg actcgcgcta cgcgaagatc
     4201 gtgcaggtga tggagcaggc cgaagcgcag cggccgcggt tgcgaagaat cgcggaccgg
     4261 ctgggcggct ggtacacgtt gttcgcgctg gtcatcgcct tcgccggatg gatcatcggc
     4321 cacgatccgc gccgcttcct ggccgtgctc gtgatcgcca cgccctgccc cctgctcctg
     4381 gcgatccctg tggcgatcat cggcgcgatc tcgcttgccg catcgcgcgg catcatcatc
     4441 aagaatccgt cgatgctgga gcgtatcgac cgctgccgga cgatcatctt cgacaagacc
     4501 ggaacgctga cctacggcag gccgtcgctg accgatgtga tctgcgcgcc cggcgtgacg
     4561 cgtgaacaag cgctgcggct ggccacgagc ctggagcagt actcgcggca tcctctcgcc
     4621 atcagcatcc tcgaagctgc gcgcgccgag gggatcgccg cggtcaacgt gtcggagatc
     4681 agcgaacgac ccggcgaagg attgcgtgga cgtatcgacg gcgattcggt cgagatcacc
     4741 gggcgcgcga aggtcacatc gaaccgcgtc atcgcgcagt tgccgcctca tgccgccggg
     4801 atggaatgcc tcgtccttcg caacggcgaa tacgcggcca cgctgcgctt tcaggatgtt
     4861 ccgcgcgagg agagccgttc gttcgtcgat cacctcatgc cgcatcatca ggtcacgcgc
     4921 gtgatgctcg tctccggcga tcgcgacacc gaggtgcagc atctggccac gctggtcggt
     4981 atcgagaacg ttcactcggg aacgagtccc gaggaaaagc tggccatcgt ccgcgacgag
     5041 gtcgccaaag gtccgaccct tttcctcggc gacggcatca acgacgcgcc ggcgatggag
     5101 gccgccaccg tcagcgtcgc cttcggccac aatagtgaca tcaccgccga agcagcggat
     5161 gcagtcgtgc tggaaccgtc gctcgccgcg gtcgacgagc tgattcacat cggccgccgc
     5221 atgcgccgca tcgccctgca aagcgccgcc ggcggcatga ccctgagcat gatcggcatg
     5281 ctcgccgccg cagccggttg gctcgcacca gtgccgggag cgatcgcgca ggaggtgatc
     5341 gatgtgctgg cggtactcaa cgcgctacgg gtcgcggcac cgcgaatgcg actcaacgat
     5401 ctcggtcatg attagcacca cagagacaca gaggtcacag aaatcacctc tgtgtcctca
     5461 gtgtctctgt ggttgattct gtccaatcgt tcgcctaccg cgcccagtga ccgtctacga
     5521 agtagtagcc gcggcgggtg tgcacccagt gagccggaac ccagaccgcg cttcggtatg
     5581 gagcacgtgc ccacgcgccg ctcgtccaga cgtagcggtt gccgtcccag cgatgatagc
     5641 cctgctgcca gacgtaaccg ggccgcacca gcactggccg ctgctcgacc acgagcggcg
     5701 gcggtccaat tcgcagatag atgcgcgtcc gcgcttccga aatcgggacg atcagcatcc
     5761 cagtgagaac cgccattgaa aggaatcggg aaaggattct cattgtgatt tgcctccgca
     5821 gttttatttg cgaagaggat gccggaggtg cgcaacgttg gggtcggctc gcccgcgttt
     5881 ttcgaagtgt taccgagctc ttcccgctct cggccgctgc ccagtccgcc gcaacgcatc
     5941 gggcccgacc cactcgtcgt ccgcaacctc gtagccgaag tagtgaacgc gataccggtg
     6001 attcttcgcg tcgatgatcc gcgccttgta ccagtcgccg tcggacctca cttccaccct
     6061 gcggccgatg gcggcatcgc tcctcgtctg tg
//
LOCUS       DATLVW010000277         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_589823, whole genome shotgun sequence.
ACCESSION   DATLVW010000277 DATLVW010000000
VERSION     DATLVW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589823"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1305
                     /locus_tag="VNN25_08590"
     CDS             19..1305
                     /locus_tag="VNN25_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXH91624.1"
                     /translation="MTENTGSEKLIRAVGFWGLVAMCINAVVGSGVFLLPTESYKLLG
                     PFSLWAPLIFALPVFILVLCFAEAASHFSEPGGAYLYAKTAFGDFIGFETGWMNWLAR
                     VTSLAALSNGFVVSLARIFPSLGTPIARAAVIIGSLAILTAIHYVGVKYGAASIYVFT
                     LGKLIPLVGFIVVALIAWKHNPIPASMHVPGPGTDWSGAALFMLFAYAGFENMGVPAG
                     EFRNPRKELPRALLVGTLAIAAIYVLAQLGAMSALPDLSKTATPIADAAAALIGSTGA
                     IIVTLGALLSMAGTNSGTVLEGSRMLYAISLGRPRLRALSYVHPSFRTPSVAIIIHVI
                     FATALALGGSFAKLAMLSAVARLTTYLFTCAAVPRLRKLNEGFQTPTLLLPILGTLIS
                     LALFFTLNAFNFVAAAIALAVGAGIYAISPVAAETT"
     gene            complement(1527..>2214)
                     /locus_tag="VNN25_08595"
     CDS             complement(1527..>2214)
                     /locus_tag="VNN25_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91625.1"
                     /translation="WLPIARENDAYNNPLRPRYATELEAYLDPQRTFLHTLDGGIADN
                     IGLRGPLHALVSTDAFVQPQDEGRLTGMTFMPMISHAPGIRAIDRVLVIVVNAGASGG
                     VKIDNTSREPKLPVVLGGVIGAPMDNYSFDSQQLLIQTFVPRIGTGVRYYPVLISFPM
                     LRDKNLRDIVNNVGTSFDALTNEQLSGLKQAADVLVHQDPCFQQFIRDVNAQPTPPGS
                     PICTSAPPLP"
BASE COUNT          367 a          733 c          675 g          439 t
ORIGIN      
        1 ccccaaagga aaagaacaat gaccgaaaac accggctccg aaaagctcat ccgcgctgtc
       61 ggcttctggg gcctcgtggc gatgtgcatc aatgccgttg tcggcagcgg agtgtttctg
      121 ctgcccaccg agtcgtacaa actcctcggg ccgttctcgc tctgggcgcc gctgatcttc
      181 gcgctgccgg tgttcatcct cgtgctctgc ttcgcggagg ctgcgagcca tttcagcgaa
      241 ccgggcggcg cgtatctcta cgcgaagacc gcgttcggcg acttcatcgg tttcgagaca
      301 ggatggatga actggctggc gcgcgtcacg tcgctggcgg cgctgtcgaa cggcttcgtc
      361 gtctcgctgg cacgcatctt cccctccctc gggacgccaa tcgcgcgtgc ggccgtcatc
      421 atcggctcgc tcgccattct cacggccatt cactacgtcg gcgtcaagta cggtgccgcg
      481 tcgatctacg tctttaccct cggcaagctg attccgctgg tcggcttcat cgtcgtcgcg
      541 ctcatcgcct ggaagcacaa tccgattccg gcgtcgatgc acgtgcccgg acccggcacc
      601 gactggagcg gcgccgcgct cttcatgctc ttcgcctacg ccggtttcga gaacatgggc
      661 gttcccgccg gcgagttccg caatccgcgc aaggagctgc cgcgcgcgct gctggtcgga
      721 acgctggcca tcgccgcgat ctacgttctg gcgcagctcg gcgcgatgtc ggcgctgccc
      781 gacctctcga agactgccac gccgatcgcc gatgccgcag ccgcgctcat cggcagcacc
      841 ggcgcgatca tcgtcaccct cggcgcactg ctatcgatgg ccggtacgaa ctccggcacg
      901 gtgctcgaag gctcgcgcat gctctacgcc atctccctcg gccgccctcg cctgcgcgcg
      961 ctgagctacg ttcatccgag cttccgcacg ccgtcggtcg cgatcatcat ccacgtgatc
     1021 ttcgccaccg ccctcgccct cggcggctca ttcgcaaagc tggccatgct ctccgccgtc
     1081 gcgcgattga cgacgtatct gttcacctgc gccgcagtgc cccgcctccg caaactgaac
     1141 gaaggtttcc agacccccac cctcctgcta cccatcctcg gcactctgat ctcgctggcg
     1201 cttttcttca cgctcaacgc cttcaacttc gtcgccgcag ccatcgcgct ggcggtgggg
     1261 gcggggattt atgccatctc gccggtggcc gctgagacga cgtgataaag gattgaccgt
     1321 ttcgccgggg agactgcagg gcgagattca ggccggatcc aaccatcgag gcgggacgcc
     1381 ccgatggccg cgggcgagac gcccacactc cttcgtcgta aggagtgcgg tcgtctcgac
     1441 cgcgggcact cgggcgtcct cgccggagtg ctgtcagggc ggaggtcgaa gtaaatcatc
     1501 cctgaaccga gcggatcaac ttagtttcac ggcaatggcg gcgccgaagt acagatcggg
     1561 cttccgggcg gcgtcggctg cgcgttgacg tcgcggatga attgctggaa gcagggatcc
     1621 tgatgaacaa ggacatcggc ggcttgtttg agtccgctga gctgttcgtt cgtcaacgcg
     1681 tcgaaactcg tgccgacgtt gttgacgata tcgcgcaggt tcttgtcgcg aagcatcgga
     1741 aagctgatca ggaccgggta gtagcgcacg ccggtgccaa tgcgcggaac aaaggtttga
     1801 atcagcagtt gttggctgtc gaatgaatag ttgtccatcg gtgctccgat gacgccgccg
     1861 aggacgaccg gcaactttgg ctcgcgcgaa gtgttgtcga tcttcacccc tcccgaggct
     1921 ccggcgttga ccacgatcac aagcacgcga tcaatcgcac gtatccctgg cgcatggctg
     1981 atcattggca tgaacgtcat tccggtgaga cggccctcgt cttgaggctg cacgaacgcg
     2041 tccgtggaga ccagcgcgtg aagcggacca cgcagtccga tgttgtcggc gatgccaccg
     2101 tcgagggtgt gaagaaacgt ccgctgcggg tcgagatacg cctcgagctc cgtcgcataa
     2161 cgcggacgca aagggttgtt gtatgcgtca ttctcgcgcg cgatcggaag ccag
//
LOCUS       DATLVW010000278         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_179304, whole genome shotgun sequence.
ACCESSION   DATLVW010000278 DATLVW010000000
VERSION     DATLVW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179304"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VNN25_08600"
     CDS             <1..619
                     /locus_tag="VNN25_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXH91626.1"
                     /translation="LKIRNAEQGLHALEMRAPYDGILVLQHDWRGDIVRVGDSVWPGS
                     PIGEIPDLNSMKAEVFVLEADAAGLAVGEKATLSVEGKPGSVITGKITQVDKLARPRF
                     PRVPVQYFGATITLDKTDPSMMKPGARVRAVLDVEDRQSAFSIPRQAIFEKQGKKLVY
                     CKRRGKFEPVVIEIGSSSAGRVVVMKGLKNGDEIALRDPTEEKDE"
     gene            731..1966
                     /locus_tag="VNN25_08605"
     CDS             731..1966
                     /locus_tag="VNN25_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91627.1"
                     /translation="MTGTETFTTALQNLATHKLRTALTMLGMIFGVGAVIAMLSIGAG
                     GEKKALEAIGRLGLRNVIVRAKTVKPEERAELRKKSLGVSLRDGEAISEAVPGVEMVL
                     PRVEVKPYKIIAPGTKTKAKVLGISWRYPRVATVSLAEGRMFDMRDEQEYAQVCVIGQ
                     EVRRSLFGYGPAIGKHLKVNDVWLEVIGLFTGDEPGSASSKDVPVASNAQEIWIPVST
                     AMRKFDRDPLDAPVDELLVELKEGASTRDAATLIRPLLERLHGGADDYEIVVPEALLE
                     QSRQTQRIFSIVMGSIAGISLLVGGIGIMNIMLASVMERTREIGIRRAIGARKRDIRA
                     QFVIESFTISLIGGISGIIIGIILARLVAAYAGWPTVITVSSLLLSCGVSITVGLVSG
                     IYPAARASELDPIQALRYE"
BASE COUNT          411 a          637 c          733 g          400 t
ORIGIN      
        1 cctcaagatc cgcaacgccg agcagggatt gcacgcgctg gagatgcgcg cgccgtatga
       61 cggcattctg gtgctgcagc acgactggcg cggcgacatc gtccgcgtcg gcgactcggt
      121 ctggccgggc agtccgatcg gcgagattcc tgatctcaac tcgatgaagg cggaggtctt
      181 cgtgctcgaa gctgacgctg ccggtctcgc tgtcggggag aaggcgacat tgtcggtcga
      241 agggaagccg ggctcggtga tcaccggcaa aatcacgcag gtcgacaaac tggcccggcc
      301 gcgcttcccg cgggtgccgg tgcagtactt cggcgcgacg atcacgctcg acaagaccga
      361 tccgtcgatg atgaagccgg gcgcacgcgt ccgcgctgtg ctcgatgtcg aagaccgcca
      421 gagcgcgttc tcgatcccgc gccaggccat cttcgaaaag cagggaaaga agctcgtcta
      481 ctgcaagcgg cgcggcaagt tcgagccggt cgtcatcgag atcggttcgt cgtctgcggg
      541 gcgtgtcgtt gtgatgaagg gattaaagaa cggcgatgag attgcactgc gcgatccgac
      601 cgaggagaag gatgagtgat ggcttgcttg gttgccgcgt ggtcagtaaa caaaggcaac
      661 cacaaagaca cagaggacac aggggttttc tctgtgacct ctgtgcctct gtggtgaagg
      721 ttttttcaaa atgaccggaa ctgaaacctt taccaccgcg ctgcaaaacc tggccacgca
      781 caagctgcgg acggcgctga cgatgctggg gatgatcttc ggcgtcgggg ccgtgatcgc
      841 gatgctgtcg atcggcgcgg ggggcgagaa gaaggcgctc gaagcgatcg ggcgcctcgg
      901 acttcgcaac gtcatcgtcc gcgccaagac cgtcaagccg gaagagcgtg ccgagctacg
      961 caagaaatcg ctcggcgttt cgttgcgcga tggcgaggcc atcagcgagg cggtgccagg
     1021 cgtcgagatg gtccttccgc gcgtcgaggt gaagccgtac aagatcatcg cccccggcac
     1081 caagaccaag gcgaaagtcc tcggcatctc ctggcgctat ccgcgcgtgg ccacggtgtc
     1141 gcttgctgag ggacgcatgt tcgacatgcg cgacgagcag gagtacgcgc aggtctgcgt
     1201 catcggccag gaagtgcgcc gcagtctctt cggctacggc ccggccatcg gcaagcatct
     1261 caaggtcaat gatgtctggc tggaagtgat cggtctcttc accggcgacg agccgggatc
     1321 ggcatcgtcg aaggacgttc cggtcgcatc gaacgcgcag gagatctgga ttccggtgtc
     1381 gacggcgatg cgcaagttcg accgcgatcc actcgatgcg ccggtcgatg agctgctcgt
     1441 ggagttgaag gaaggcgcgt ccacgcgcga cgccgcgacg ctcatccgtc cgttgctgga
     1501 gcggctgcac ggcggcgcgg acgactacga gatcgtggtg ccggaggcgc tgctcgaaca
     1561 gagccggcag acgcagagga tcttttcgat cgtgatgggc tcgatcgccg gcatctccct
     1621 cctcgtcggc ggcattggca tcatgaacat catgctggcc tcggtgatgg agcgtacgcg
     1681 cgagatcgga atccgccgcg ccatcggagc gcgcaagagg gacatccgcg cccagttcgt
     1741 gatcgagtcg ttcaccatca gcctgatcgg cggcatctcc ggcatcatca tcggcatcat
     1801 cctggcgcgc ctcgtggctg cgtacgcggg atggccaacg gtgattacgg tgagctcgct
     1861 gctgctgtcg tgcggcgtct cgatcactgt ggggttggtg tcggggatct atccggccgc
     1921 gagggcatcg gagctcgatc cgattcaggc gctgcgatac gagtaggttt ttttatccgc
     1981 agattacgca gatagacaca gatgggaacg tgtgtcccaa tgctagggtt ctcaccggca
     2041 accgcatgtc tgaacttggc tggaaatacc tcaccgctgc cgacaagcag gagattctgc
     2101 gcggcgtacg cgatggcgtc gcgtacggtg ggccgtacca cgtcgagatc catccggccg
     2161 accgctgcaa catcgagtgc t
//
LOCUS       DATLVW010000279         6892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_463552, whole genome shotgun sequence.
ACCESSION   DATLVW010000279 DATLVW010000000
VERSION     DATLVW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463552"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VNN25_08610"
     CDS             <1..1141
                     /locus_tag="VNN25_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXH91628.1"
                     /translation="ITPLTLFHFHQYALGGSVITMLLTPIVFAMLVLSMAICAIPCDA
                     LFAALGFLHQLCTFVNGVAAPVWGFFAAPPLIALVTGYVAAILAVAFLRGRIRALAIL
                     AAMLIPIGGAIAVARQDVATPRLTMLDVGQGDSLLLRSPKHVVLIDAGGRLGDAHFGE
                     SELLPMLLDRGVRRIDVAVLTHVHPDHCGGLPAVLANLDVGSMWISPRRFRGDCAQRV
                     LEACSLRSIPIHLVRDGETRGVGPMTLRAFVADRTFKHSPENNSSVVLLATIGRWKAL
                     LTGDVERDAEESLASQDIQADVLKVGHHGSRTSTTTSLLDAVQPRIALISCGLHNTFG
                     HPHPAVVESLRAHGVRTWITARDGTIDLDFLPGHLVVHRQFDTPR"
     gene            1165..1491
                     /locus_tag="VNN25_08615"
     CDS             1165..1491
                     /locus_tag="VNN25_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91629.1"
                     /translation="MSSTLLRLGLWIILLVVILYVLQQTYEEAPFVDYFAPAMLEKAL
                     ILGGLLVAAGIVFRLMEGGAKVVTKNRCVTCKAPIEKGAIYCRQHLRSVLSREDDRTH
                     MTRVRK"
     gene            1503..2435
                     /gene="miaA"
                     /locus_tag="VNN25_08620"
     CDS             1503..2435
                     /gene="miaA"
                     /locus_tag="VNN25_08620"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXH91630.1"
                     /translation="MTNAAIVIAGPTGVGKSDLALRLAESLGGEIVNYDSVQIYRGFD
                     IGSAKPSAEARGRVPHHLFDIVDANAEFNASAYATLARTTCEEIAARGRRAILVGGTF
                     FYLRALLHGLPPMPARDAVVRERLRRIAVRPRGAERLHRWLSKVDPQSGKRIASADRH
                     RVERALEVWLTSGRPISEWERPGSGGTEEMAATKIGLQMERSRLVERLDERVTAMYAA
                     GLIDETRSLLTQFPRTARPFGAIGYAEAAAVMSGEIDVDAAIAETRRRTRAYAKRQMT
                     WLRSERNVQWIDVTSCDDPFPAAMRLAEMSEGSD"
     gene            2432..3718
                     /locus_tag="VNN25_08625"
     CDS             2432..3718
                     /locus_tag="VNN25_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH91631.1"
                     /translation="MKTRTIFLLITAAVVLGRCAPAPPVVAPSAPQAGDRFIIDPRTG
                     YDAQPAPADAKRFDEAWHAILAGDYTTARKRLETIRTKEPAYVPAQLAEAAIDLREGK
                     TDAARPIVERASAHHPHYTAALVYAAEIAIVEKRSREAYNIYRDLTTRPGAPDFVAER
                     IAGLRTALFDQLYGAALAAPDEDAVRLLRDALSINPEGNGARILLAQKLVGQHKYDEG
                     LVTLDPLLKGADADRSEVQEALAEIELNRGRYESAIARYERLAKRDRRFAARLDEIKQ
                     NYAESNMPSQFRRAIESESVTRGDLAVLMYWKVASVRFAANLATPPIAVDVSETPGRD
                     EIVRAIALGIYQVDPVTRRVNPFSAVNAGALARTSARLLALRGAACARGAGNDPQRVL
                     AACAIVDPTLGAGAESPVSGRAAAGVMEQVDRAISR"
     gene            complement(3793..4416)
                     /locus_tag="VNN25_08630"
     CDS             complement(3793..4416)
                     /locus_tag="VNN25_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91632.1"
                     /translation="MTADASPETYFQEIEAHFALRRGTPFILNAKDWALMKKWREEGV
                     PLPVVIEAIDAVFERNETSGRKKVISSLSYCRHSIKELWDERRTLYVGGGEVTPESGP
                     EALLDALAVQLEASEIAAPFGARVRALASEKSVPKIEERLIDLERELIDAFIAAAPSE
                     IEAIRADIATALADAPKLDAKTRARTEEANLRRLVREKFGLPRLTLF"
     gene            4507..6162
                     /locus_tag="VNN25_08635"
     CDS             4507..6162
                     /locus_tag="VNN25_08635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH91633.1"
                     /translation="MEPIAPRAEVTVYSPLFSPFRQQLQGTAISIGRASDCSIPIKDR
                     YLSRKHAEIIVERGAWILKDCGSANGTYLNGARMERDHPLRTGDRIRLGDTEIVFETA
                     EHNTDRMLAVADTSPSMTISIPVNEIEQPGGRSDTGDLAKLKTLNLLARELIEDRPTE
                     ELFGFIVDRVLEHTEASRAALGLLGPDGKSFVNVEVRRQDRSDSSELRISHTVLSEVV
                     EEKKALAFMDVSVDEKLSRAQSIIMQGIRSILCAPLMIGDAVVGVLYVDYLFTQRKIS
                     EEDVRLVAQIARFAAIKLETTRLREEAIQKRIMDEELKTASVIQRGLLPPAPSGIEGY
                     TFSGANRPCRTVSGDYYDFVRRPDGRIYFVIADVSGKGITAGLMMAGLQASFRIFCKN
                     DPTPAALVSQLNDALKDTLPQSKFVTLFLGRLHLATGVIEYANAGHTPPLWLRSDGAE
                     ELGETDLVLGIVTRADFRSHMLQLAPGDSLILFTDGVTEAENEEGQDLGSLDHVRKRL
                     SEMHGDDADALTSAIEAVVFDHMGDAPLADDVTLLVVSRDREL"
     gene            6350..6706
                     /locus_tag="VNN25_08640"
     CDS             6350..6706
                     /locus_tag="VNN25_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91634.1"
                     /translation="MANVVGAWTVTTDWGCDGSITGSFTQTFNADGTWTSSPFVHNGR
                     WFQVEGLVAWTFSDVANLVYAANLSGSWMAGAQGYETAGGQKGCFGARRAGIPSAKAE
                     VAAKAEHGAHDPALGK"
BASE COUNT         1249 a         2156 c         2192 g         1295 t
ORIGIN      
        1 gatcacgccg ctgacgctct tccactttca ccagtacgcg ctcggcggat cggtgattac
       61 gatgctgctg acgccgatcg ttttcgcgat gctcgtgctg tcgatggcga tctgcgccat
      121 tccgtgcgac gcgctcttcg cagcgctggg gtttctgcac cagctctgca ccttcgtcaa
      181 cggagtggcg gcgccggtct ggggcttctt cgccgcgccg ccgctgatcg cgctcgtgac
      241 cggttatgtg gctgcgattc tcgcggtcgc gtttctgcga ggccggatcc gcgcactcgc
      301 gattttggct gcgatgctga ttcccatcgg cggtgcaatc gccgtcgcac ggcaggatgt
      361 cgcaacgccg cgtttgacga tgctcgacgt cggtcagggc gattcgctgc tgcttcggtc
      421 gcccaagcac gtcgtcctga tcgacgccgg cggccggctt ggcgatgcgc atttcggcga
      481 gagtgaattg ctgccgatgc tcctcgaccg cggcgtgcgc cggatcgacg tcgccgtgct
      541 gacgcacgtg catccggatc actgcggcgg cctgccggcg gtgctcgcca acctcgacgt
      601 cggcagcatg tggatctctc cgcggcgttt tcgcggcgac tgtgcgcaac gcgttctgga
      661 agcgtgctct ctccgatcaa tcccgattca tctcgtccga gatggagaga cacgcggggt
      721 tgggccgatg acgcttcgcg cgttcgtcgc cgatcgcacg ttcaagcact cgccggagaa
      781 caacagctcg gtcgttctgc tcgcgacaat cggccgatgg aaagcgctac tgaccggtga
      841 cgtcgagcgc gacgccgagg agtcgcttgc atcgcaggac atccaggcgg acgtgctcaa
      901 agttggtcac cacggtagcc ggacctcgac cacaacctct cttctcgacg cagtacagcc
      961 gcgtattgct ctcatttcgt gcggattgca caacacgttc gggcatccgc atcccgcggt
     1021 cgtcgagtcg ttgcgcgcac acggcgtgcg aacatggatc accgctcgcg atgggacgat
     1081 cgatctcgat ttcctgcccg gccacttggt ggtgcatcgg caatttgaca ctccgcgatg
     1141 agcgtcctac agtgattttt tcgcatgtca tcgaccctct tgcgcctagg gctctggatc
     1201 attcttctcg tggtgatcct gtacgtactc cagcagacct atgaagaggc gccgttcgtc
     1261 gactacttcg cccccgccat gctcgagaag gcgctgatcc tcggcggtct tctcgtcgcc
     1321 gccggcatcg ttttccggct catggagggg ggagcgaagg ttgtcacgaa gaaccgttgc
     1381 gtgacctgca aggctccgat cgagaagggc gccatctatt gccgccagca cctccgcagt
     1441 gtcctgagcc gcgaagatga tcggacgcac atgacgcgcg tacgaaagtg acgcctgcgc
     1501 aggtgactaa cgctgcgatc gtcatcgccg gccccacggg agtcggcaag tccgacctcg
     1561 ctctgcgtct agccgaatcc ctaggcggag agatcgtcaa ctacgactcc gtgcagatct
     1621 accgcgggtt cgatatcggg agcgcgaaac cgtcagccga ggcgaggggg cgggtgccgc
     1681 atcacctgtt tgacatcgtc gatgccaacg ccgagttcaa cgcctcggca tacgcaaccc
     1741 tcgcgcggac gacgtgcgag gaaatcgccg cccgcggccg tcgcgcgatc ctcgtcggag
     1801 ggacgttctt ctatctgcgc gcactgctgc acgggttgcc acctatgcct gcgcgcgacg
     1861 ccgtggttcg cgagcgcctt cgccgcatcg cggtacgtcc gcgcggagca gaacggctgc
     1921 atcgctggct ctcgaaggtt gacccgcaaa gtggtaaaag aattgcgtcg gcggaccggc
     1981 accgagtcga gcgcgctctt gaggtctggc tgaccagcgg acggccgatc tcggaatggg
     2041 agcgccccgg aagtggcggg acggaagaga tggcggccac gaagatcggt ttacagatgg
     2101 agcgaagccg cctcgtcgaa cggctcgacg agcgggtcac ggcgatgtat gcggccggac
     2161 tcatcgacga gacacgcagc ctgctgacgc agtttccgcg aacggcacgc cccttcggcg
     2221 cgatcggata cgctgaggcc gcggcagtca tgagcgggga aatcgatgtc gatgccgcga
     2281 tcgctgaaac gcggaggcgt acacgcgcgt atgccaaacg acagatgaca tggctgcggt
     2341 cggagcgcaa tgtgcagtgg attgacgtaa cgtcatgtga cgatcccttt ccagccgcga
     2401 tgcggttggc ggaaatgagt gagggcagcg attgaagacg aggacgattt tccttctgat
     2461 tacggcagcg gttgtactgg gacgctgtgc gccggcgccg ccggttgttg caccgtctgc
     2521 gccgcaggca ggcgaccgat tcatcatcga tccacgaacc ggttatgacg cacagcctgc
     2581 gccggcagat gccaaacggt tcgatgaggc gtggcacgcg attctcgcgg gcgattacac
     2641 aacagcgcgc aagcggctcg agacgattcg caccaaagag ccggcttatg taccggcgca
     2701 acttgccgaa gcggcgatcg acttgcggga gggaaagacc gacgccgccc gtccgatcgt
     2761 ggagcgtgca agtgctcatc atccgcacta caccgccgcg ctggtttacg ccgcggagat
     2821 cgctatcgtc gagaaacggt cgcgtgaggc gtacaacatc tatcgcgacc tcacgacgcg
     2881 tcccggtgcg ccggacttcg ttgccgagcg catcgccggg ctgcggacag cgctctttga
     2941 tcaactgtat ggcgccgcat tggccgcgcc ggacgaggac gcggttcggc tccttcgcga
     3001 tgcgctctcg atcaaccccg aggggaacgg agcgcgcatt ctgctggcgc agaagctcgt
     3061 cgggcagcat aagtacgacg aaggactggt cacgctcgat ccgttgctga aaggtgccga
     3121 cgccgatcgt tcggaagtgc aggaagcgct ggcggagatc gagctcaatc gtgggcgcta
     3181 cgagtctgcg atcgcgcgct acgagcgtct ggcaaaacgc gaccggcgat tcgccgcgcg
     3241 cctcgacgag atcaagcaga actatgcgga gtcgaacatg ccttcgcagt tccgccgcgc
     3301 catcgagagc gagtcggtaa cgcgcggcga tctggccgtg ctgatgtatt ggaaggtggc
     3361 ctcggtgcgc ttcgccgcga atctggccac gccgccgatc gccgtcgatg tgagcgaaac
     3421 tcccggccgt gacgagatcg tccgcgccat cgccctcggt atctaccagg tcgacccggt
     3481 cacgaggcgc gtcaatccgt tctcggcggt caacgctgga gcgctggcgc gaacctccgc
     3541 gcgcctgctc gcactgcgtg gtgcagcctg cgcgcgcggc gccgggaacg atccgcagag
     3601 agtgctcgcc gcctgcgcga tcgtcgatcc gacactcggc gccggcgccg agtcgcccgt
     3661 gagcgggcgt gcggcggcag gggtgatgga gcaggtggat cgggcgattt cgcggtagga
     3721 gcttcccacc acagagacac agaggacaca gagaaggctc atttctgtgt cctctgtgtc
     3781 tctgtggttc attcaaaaca acgtcagccg agggagtccg aacttctcgc gcaccaatcg
     3841 ccgcaggttc gcttcttccg tccgcgctcg tgtcttcgcg tcgagtttcg gcgcgtctgc
     3901 gagggcggtg gcaatgtcgg cacggatcgc ttcaatctcg gatggcgcag cggcgatgaa
     3961 tgcatcgatc agctcgcgct cgaggtcgat gagccgttcc tcgatcttcg ggacgctctt
     4021 ttcggaagcc agcgcgcgga cgcgggcacc gaacggcgca gcgatctccg atgcttccaa
     4081 ctgcacggcc agcgcgtcga gcaacgcttc ggggccggat tccggcgtta cctcgccgcc
     4141 accgacatag agcgtccgtc gctcgtccca caattctttg atcgagtgtc ggcaatagct
     4201 gaggctggag atgaccttct tccggccgct ggtctcattc cgttcgaaga cggcgtcaat
     4261 cgcttcgatc accaccggta gcggcactcc ctcctcgcgc cacttcttca tcaacgccca
     4321 gtccttcgcg ttgaggatga acggcgtgcc gcgccgaagg gcgaagtgcg cctcgatttc
     4381 ctggaagtac gtttcgggcg aagcgtctgc ggtcacggtg gaaacgtacc atgcgcgagt
     4441 caggcagccg cgctacccgg caacctcagt tacaatgccc gctcgcttca aagcctgcga
     4501 cccactatgg aacccatcgc ccctcgcgct gaggtcacgg tctattcgcc gctgttctcg
     4561 cccttccggc aacagttgca aggcacggcc atttcgatcg gccgcgcgtc cgactgctcg
     4621 attccgatca aggatcgtta tctctcccgg aagcacgccg agatcatcgt cgagcgaggt
     4681 gcgtggattc tcaaggattg cggcagcgcg aacggcacct acctgaacgg tgcgcgtatg
     4741 gagcgcgatc atccgctgcg aactggtgac cggatccggc tgggcgacac ggagatcgtc
     4801 ttcgagacgg ccgagcacaa caccgaccgg atgctcgcgg tggccgacac ttcgccgagc
     4861 atgaccatct cgatccctgt aaacgagatc gagcagccgg gcggcaggtc cgacacgggc
     4921 gatctcgcca agctcaagac gctcaacctg ctggcgcggg agctcatcga agaccgtccc
     4981 accgaggagc ttttcggctt catcgtcgac agagttctgg agcacaccga ggcatcgcgc
     5041 gcggccctcg gactactcgg cccggacggc aaatccttcg tcaatgtcga agtgcgccgg
     5101 caggaccgca gcgactcgtc ggagcttcgc atcagccata cggtgctgag cgaagtggtc
     5161 gaggagaaga aggcgctggc attcatggac gtcagcgtcg acgagaagct gagccgcgcg
     5221 cagtcgatca tcatgcaggg catccgctcc attctctgcg cgccgctgat gatcggcgat
     5281 gccgtcgtcg gcgttctcta cgtcgactat ctcttcacgc agagaaagat ctccgaggag
     5341 gatgtgcgcc tcgttgcgca gatcgcgcgg ttcgcggcga tcaagctgga aacgacccgt
     5401 cttcgcgagg aagcgatcca gaagcgcatc atggacgagg agctgaagac cgcctctgtg
     5461 attcaacgcg gccttcttcc gcctgcgccg tccggcatcg aaggctacac gttctctggc
     5521 gcgaaccggc cctgccgaac ggtcagcggc gactactacg acttcgttcg ccgccctgac
     5581 ggccggatct atttcgtcat cgccgacgtc agcggcaagg ggatcactgc tggtctgatg
     5641 atggccggcc tgcaggcatc gtttcgcatc ttctgcaaga acgatccaac accggccgcg
     5701 ctggtttcac aactgaacga tgcgttgaaa gacacgctgc cgcagtcgaa gttcgtcacg
     5761 ctttttctcg gacgactgca cctggccacg ggcgtgatcg aatacgcgaa cgccggccac
     5821 acgccgccgc tctggctgcg cagcgatggt gccgaagagt tgggcgaaac ggatctcgta
     5881 ctgggaatcg tgacgcgtgc cgatttccgt agtcacatgc tgcaactcgc accgggcgac
     5941 tccctgattc tcttcacgga tggagtgacc gaggcggaga acgaagaggg gcaggacctg
     6001 gggagcctcg atcacgtgag gaaacgtctg tcagaaatgc acggcgacga cgccgacgcc
     6061 ctgaccagtg ccatcgaagc cgtggtgttc gatcacatgg gtgacgcgcc gctggccgat
     6121 gacgtgacgt tgctggttgt gagtagagac cgggagttgt gagctttccc gagttctgcg
     6181 gaaccgtgcg gatacgtcat ctcatctgaa gtttcgccga aagctgccga cgtcctttcc
     6241 attcttttgc gctgatgcca ttcccgatgg cttgtcaaac cgaaagcgtg aacttatctt
     6301 ccgtacctgc ggactaaacc gcaaagcaac catacagaag gaggtctcta tggcgaatgt
     6361 tgtcggtgcg tggacggtca cgacggactg gggctgtgat ggttccatta ccgggagttt
     6421 cacccaaacg tttaacgctg acgggacgtg gacctcgagt cctttcgttc acaacggaag
     6481 gtggtttcag gtcgagggtc tcgtcgcctg gacgttcagc gacgtagcca atctcgtgta
     6541 cgccgcaaac ctctctggca gctggatggc cggcgcacag gggtatgaaa ccgccggcgg
     6601 gcagaagggc tgcttcggcg ctcgaagagc aggcattcct tcggcgaagg cggaagtcgc
     6661 cgcgaaagca gaacacggcg cccacgatcc agcattggga aaataagctg ctggcagtag
     6721 agcgacagcg aaggcccaaa gactgctgcg taggccggcc tctcggttcc gatgggatcg
     6781 ggaggccggt caagattggc cgtcggcttg atcggttggg attcgggggt cgcgagtcgg
     6841 gagtcgtaaa cgaacgcaac ccacccccca ctcccgactc cccacccccc ac
//
LOCUS       DATLVW010000280         4875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_259758, whole genome shotgun sequence.
ACCESSION   DATLVW010000280 DATLVW010000000
VERSION     DATLVW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259758"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /gene="rmuC"
                     /locus_tag="VNN25_08645"
     CDS             complement(<1..647)
                     /gene="rmuC"
                     /locus_tag="VNN25_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXH91635.1"
                     /translation="MAVLSLIAAVVGIVLLLLLSWIVLRGSRRLIAVEATQSREESRA
                     EAIALRQELTAAMGTLATSLRADANTARVEAAENRRSHREEITRTLDSSSQRVIAAVS
                     DHGTTQRQDLEAIRAQVVMLTESNEQRIGDVRRTVDERLKEIAAGNARELQEMRATVD
                     EKLQGTLNQRLGESFALVTQRLEEVHRGFGEMQALATGVGDLKKVLSNVRSRGIWG"
     gene            complement(707..1114)
                     /locus_tag="VNN25_08650"
     CDS             complement(707..1114)
                     /locus_tag="VNN25_08650"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HXH91636.1"
                     /translation="MTEERSFLNDGGVYVSNTRVVIQGTTYATANITSVRKHIVPAKN
                     GCAVVFVIFGALGVLGGLASVLGGNGDERWATLVLFAILLIIGIFWLRSLHPTYHLML
                     ATAAGERQGMTSNDEGVVDRVTAAVADAIVHRG"
     gene            complement(1387..1938)
                     /locus_tag="VNN25_08655"
     CDS             complement(1387..1938)
                     /locus_tag="VNN25_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91637.1"
                     /translation="MSDPIDPKSLAYRQTCFEIHIRPMMRLIDHDHMLYLAPEGQTLD
                     LFDYDTVKQHADAILERLQYNMPTIRYGGLWPAEWILLFERWMKEKFPRLDKYKGTYK
                     VVIDGKGMVVLTGTFATIRKRDRIWFERFTTSDSPRKYHVYREPGSGDPNVQQITIDE
                     RFPRVYGTVIVNDSDGRHELAIP"
     gene            complement(1935..3311)
                     /locus_tag="VNN25_08660"
     CDS             complement(1935..3311)
                     /locus_tag="VNN25_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HXH91638.1"
                     /translation="MNRIARSLLAIALTLAVIAPLGAQTKTRLNIDDPAFNVKLYEKA
                     VQALKDRDKDPNHKDDPNTPSKNGYKYFEEMHNGVGAMSTCQHQNEIFLTWHRAVLLQ
                     YEKALQATYPGETDNLMLPYWNWGVKPSGKYYPAAYEQPSSPLYDKNRDPETPEKPYT
                     NEQLRTMMLKTTTWRGFSGGECSVGTCSDGKPCAVCTPKFGAFESPYHNIMHNWTGGW
                     MTDDKTAAKDPIFWSFHAYIDLVFDCWQKTQKITDVGCPDCPLRALPGNPTAKDMKST
                     AALGYTYDFRDTACAEPGLNARPKVLAMASRQKKSTPYVVDLTIPRPSFVTAHVRLEG
                     LPVFADYNYSGRVFLYPATVTLASSDADFRKKYQVDRFAVWGLGDTEHAPHHEAAVTA
                     DVDASTELDYLAKKEPGAKWKIAVVVDKPSAQYESTRKNASAEVQREITFDHVSVIYD
                     RDYAKEQQ"
     gene            complement(3458..3589)
                     /locus_tag="VNN25_08665"
     CDS             complement(3458..3589)
                     /locus_tag="VNN25_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91639.1"
                     /translation="MDDREDCVFCGTDLDAADAEHEANVMEMRRAAQALREALAERG"
     gene            complement(3648..>4875)
                     /locus_tag="VNN25_08670"
     CDS             complement(3648..>4875)
                     /locus_tag="VNN25_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH91640.1"
                     /translation="MDAQRADALPVRYAGLDDIVNRVPVSVVWEITLACDLACSHCGS
                     RAGTRRRDELSTAEALDLVDQIAELGARDVGLIGGEAYLRKDWLEIVAAIRATGMRCD
                     LQTGGRNLSEERVASAAAAGLCGVGISIDGIGTTHDKLRGVTGSYDLALAALRRVRAH
                     GLSSAVNTQINAYTLPQLRELMDVIIEAGATNWQLAITTAMGNAADHPDMLLQPWQML
                     DVMPLLAELAVEGRERGLFLQPASNIGYYGPYESVIRNVLDPEIYFHGCNAGDTVMGI
                     EADGTIKSCPGLGSAYAGGNVRETRLREIWEKSEVMAFNRRRTVDDLWGFCRTCYYAE
                     TCMAGCTWTSHALMGRAGNNPMCHHRALEMDRQGLRERLVKVKDAPGRSFDHGEFEIV
                     VEPVPARAADGIVVNG"
BASE COUNT          827 a         1537 c         1538 g          973 t
ORIGIN      
        1 ccccagatgc cacggcttct cacgttcgag aggactttct tcaagtcacc gacgccggtt
       61 gcgagcgctt gcatctcacc gaaaccgcgg tgaacttcct ccagtcgctg cgtgacgagg
      121 gcgaacgatt caccgagacg ctgattgagc gttccctgca acttttcgtc gacggtcgcg
      181 cgcatctcct gaagctcccg cgcgttcccg gcagcgattt ccttgagccg ctcgtcaacg
      241 gttcgccgca cgtcgccgat gcgttgttcg ttcgactcgg tcagcatcac gacctgagct
      301 cgaatcgctt ccaggtcctg acgctgcgtc gtgccgtggt ccgacaccgc cgcgatcacg
      361 cgctgactcg acgagtcgag cgttcgcgtg atctcctcgc gatgggatcg cctgttctct
      421 gctgcctcga cgcgtgctgt gttggcgtcc gcgcgcaagg acgttgcgag cgtacccatc
      481 gccgccgtca gctcctgacg caatgcgatc gcctcagcgc gcgattcctc acgcgattgt
      541 gttgcctcaa cggcgatcaa acgtcgcgac cctcgcaaga cgatccagga cagaagaagc
      601 agcaagacga tcccaaccac tgctgcaatc aaggaaagca ccgccatgga agatcctccc
      661 gagagggaag acacggtgaa gctggaaagg tggcacgctg gccgcgctac ccgcgatgca
      721 cgatagcgtc ggcgacggcc gcggtcacgc gatcgacgac tccttcatca ttcgacgtca
      781 ttccctgccg ctcgccggcc gccgtggcga gcatgaggtg ataggtcgga tgaagggatc
      841 ggagccagaa gattccgatg atcagaagaa tcgcgaagag gacgagcgtc gcccatcgct
      901 cgtcgccgtt cccgccgagt acggaagcca gtccgccaag aactcccaaa gcgccgaaga
      961 taacgaagac aacagcgcac ccgttcttcg ccggaacgat gtgtttgcga accgaagtga
     1021 tgttcgccgt cgcataggtc gtcccctgaa tcacaacccg agtgttcgaa acatacacac
     1081 caccatcgtt gagaaacgag cgttcttcag tcacggtcct gctccttcaa tggatcgatc
     1141 ggaatgatcg atagacaccg cgcccgatgg tttgtgggga gtgtctggag cccgcgcggc
     1201 aaaaacctgc gtcgcgcggc cggacaaaaa tgcaccacag agacactgag gacgcagagt
     1261 tgaaactctg ctctgctact tctgtgtctc tgtggtgcag ggttcgccag gggttgctcg
     1321 tgcccggttt cggcacggga acgagctaat ctggaggaca ggcaaccttg cccgtcctcc
     1381 acccctctac ggaatcgcca gctcgtgcct tccgtccgag tcgttcacga ttacggtccc
     1441 atacacccgc ggaaacctct catcgatcgt gatctgctgc acgtttggat ctccgcttcc
     1501 cggctctcgg tacacgtgat acttgcgtgg cgagtcgctc gtggtgaacc gctcgaacca
     1561 gattcgatcg cgcttccgga tcgtcgcgaa tgttcccgtc agcaccacca tccccttgcc
     1621 atcgatgacc accttgtacg tccccttgta cttgtcgagc cgtgggaact tctccttcat
     1681 ccagcgctcg aacagcagga tccactccgc gggccacagg ccgccgtagc gaatggtggg
     1741 catgttgtat tgcaggcgct ccagaatcgc atcggcgtgc tgcttgaccg tgtcgtagtc
     1801 gaagagatcc agggtctgtc cctccggcgc gaggtagagc atgtgatcgt gatcgatgag
     1861 ccgcatcatc gggcggatgt ggatctcgaa acacgtttgg cggtaggcga ggctcttcgg
     1921 atcgatcggg tcgctcattg ctgctccttc gcgtagtcgc ggtcgtagat gacggagacg
     1981 tggtcgaacg tgatctcgcg ctgcacctcg gccgaggcat tcttgcgtgt gctctcgtac
     2041 tgcgctgacg gcttgtcgac gaccacggcg atcttccact ttgctcccgg ctccttcttg
     2101 gccaggtaat cgagctcggt cgatgcgtcg acgtccgcgg tgaccgccgc ttcgtgatgc
     2161 ggggcgtgtt cggtatcgcc gaggccccag accgcgaaac gatcgacctg gtacttcttg
     2221 cggaagtccg cgtcgctcga ggcgagcgta accgtggcgg ggtaaaggaa cacgcggccg
     2281 ctgtagttgt agtcagcgaa caccggcagc ccttccagcc gcacgtgcgc ggtgacgaag
     2341 gacggccgcg ggatggtgag gtcgacgacg tagggagtgg acttcttctg gcggctcgcc
     2401 atcgcgagga ccttcggcct ggcgttcagc cccggctcgg cgcaggcggt gtccctgaag
     2461 tcgtaggtgt aaccgagggc cgcggtgctt ttcatgtcct tggcagtcgg attgccgggc
     2521 agggcgcgga gcggacagtc cgggcagccg acgtcggtga tcttttgtgt cttctgccag
     2581 cagtcgaata cgagatcgat gtaggcgtga aacgaccaga agatcggatc cttggccgcg
     2641 gtcttgtcgt ccgtcatcca tccgccggtc cagttgtgca tgatgttgtg gtagggcgat
     2701 tcgaaggcac cgaacttggg tgtgcacacc gcgcagggct tgccgtcgct gcacgtcccg
     2761 acgctgcact cgccgccgct gaagcctctc cacgtggtcg tcttgagcat catcgtgcgc
     2821 agttgttcgt tcgtgtaggg cttctccggg gtctccggat cgcggttctt gtcgtagagc
     2881 ggagagcttg gctgctcgta ggcggccggg tagtacttgc cgctcggctt cacgccccag
     2941 ttccagtagg ggagcatcag gttgtcggtc tcgcccggat aggtggcctg cagcgctttc
     3001 tcgtactgga gcagcaccgc gcggtgccag gtcaggaaga tctcgttttg gtgctggcag
     3061 gtcgacatcg cgccgacgcc gttgtgcatc tcctcaaaat acttgtagcc gttcttcgaa
     3121 ggcgtgttcg gatcgtcttt gtgattggga tccttgtcgc ggtccttcag cgcctgaacc
     3181 gccttctcgt acagttttac gttgaaggcc ggatcgtcga tgttgaggcg agtcttcgtt
     3241 tgtgcgccga gcggcgcgat gacggcgaga gtgagggcga ttgcgagcag agatcgagcg
     3301 attcgattca tgttgtgctt cccctttctg tgactaggag tggtttccct acccgtagca
     3361 taaggtgact cattcgccgc aggctccaca atcgcgcgcc gaagatcttg ctgcaggtcg
     3421 ttgctatgcg tcgacgacgc ggcccacgcg cagcggctca gccgcgttcc gccagcgctt
     3481 cgcgcagcgc ctgcgccgcc cgccgcatct ccatcacgtt cgcctcgtgt tcggcgtccg
     3541 ccgcgtccag atcggtaccg cagaaaacac aatcctcccg atcgtccacg cacacgaagc
     3601 gtcggcagtc gcggcagagg cgaagaggaa ccgggatgcg gccactttca gccattgacg
     3661 acgatcccgt ccgcggcgcg cgccggcacc ggctccacca cgatctcgaa ctcgccgtga
     3721 tcgaacgagc ggccgggggc gtctttcacc ttgaccagcc gttcgcgcag gccctggcgg
     3781 tccatctcca gcgcacggtg atggcacatc gggttgttgc cggcccggcc cataagcgca
     3841 tgcgaggtcc acgtgcagcc ggccatgcac gtctcggcgt agtaacaggt gcggcagaag
     3901 ccccatagat cgtcgacggt gcgtcggcgg ttgaaggcca tcacttcgga cttctcccag
     3961 atctcgcgca gccgcgtctc gcggacgttg ccgccggcgt aggcgctgcc gaggcctggg
     4021 caggacttga tcgtgccatc cgcttcgatt cccatcaccg tgtcgccggc gttgcagccg
     4081 tgaaaataga tttccgggtc gagcacgttg cggatgaccg attcataggg gccgtaatag
     4141 ccgatgttgc tcgccggctg caggaagagg ccgcgctcgc ggccctcgac ggcgagctcg
     4201 gcgaggagcg gcatcacgtc cagcatctgc cagggctgca gcagcatgtc ggggtgatcg
     4261 gcggcattgc ccatggcggt cgtgatggcg agttgccagt tggtcgcgcc ggcctcgatg
     4321 atcacgtcca ttagttcgcg cagttgcggc agcgtatagg cgttgatctg agtgttgacg
     4381 gcggaggaga ggccgtgcgc gcggactcgg cgcagcgccg cgagcgcgag atcgtacgaa
     4441 ccggtcacgc cccgcagctt gtcgtgggtc gtgccaatgc cgtcgatcga gattccgacg
     4501 ccacacagtc ccgcggcggc agcgctggcg acacgctcct cgctgaggtt gcggcccccg
     4561 gtctgcaggt cgcagcgcat cccggtggcg cggatcgcgg caacgatctc gagccagtcc
     4621 ttcctcagat aggcctcgcc gccgatcagg ccgacgtcgc gcgcgccaag ctccgcgatc
     4681 tgatcgacga ggtcgagcgc ctcggcagta cttagctcgt cgcgccgccg ggtgccggcg
     4741 cgggaaccgc aatggctgca ggcaaggtcg caggcgagcg taatctccca gaccacgctc
     4801 accggaacgc ggttgacgat gtcgtcgagc cctgcgtagc ggacaggcag tgcgtctgcc
     4861 cgctgggcat ccatt
//
LOCUS       DATLVW010000281        10381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_507178, whole genome shotgun sequence.
ACCESSION   DATLVW010000281 DATLVW010000000
VERSION     DATLVW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507178"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VNN25_08675"
     CDS             complement(<1..935)
                     /locus_tag="VNN25_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HXH91641.1"
                     /translation="MNVIDTQVAFDAAMARVAAQPVVALDTEADSLHSYFDKVCLIQM
                     SIPGEDMIIDPLCKIDIAKFGEILANREITKILHGADYDLRILNRDFGFTIANLIDTM
                     VCSQLLGYEAFGLAALLDRHFGFKVDKKHQRADWAMRPLPPDMLEYAATDTRHLIALA
                     GRLRDELTALGRWEWAVEEFGRQEQIRFNEKDGDEEPFRRLKGLGALDRRSLAVVRAL
                     YDFRDGLAREADRPPFKIFGNDLIFEIARIKPSSTQDFNSIKGVSGYHRGRFGRDIVR
                     LVKEAMAIPDEELPEKSESKTWLRDREMEARIDRM"
     gene            complement(932..1477)
                     /locus_tag="VNN25_08680"
     CDS             complement(932..1477)
                     /locus_tag="VNN25_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91642.1"
                     /translation="MHPLRATARYVRAIESTAELLSKLRIESMFVGSVARVAWLGGMI
                     DGGSIDVLALMQPQQQNQVAMMANNRGFRVDRDEIEQTEELDLVPLMWVDEEGDVRVH
                     VLVASNALYARMFANARTIELHTATNPREIRVPAAEDLALLLSMSDELDDVRVLAAGE
                     SFDRSAFNEKLVSIGLRERVL"
     gene            1734..3575
                     /gene="recQ"
                     /locus_tag="VNN25_08685"
     CDS             1734..3575
                     /gene="recQ"
                     /locus_tag="VNN25_08685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HXH91643.1"
                     /translation="MLDRLRDLISHHWGYDSFRPLQREAMLATLERRDSLVVMPTGGG
                     KSLCYQAPALLSEHVTVVVSPLISLMKDQVDGLRECGVAAGQLNSSQRSDQQREIERA
                     VLDGKIKLLFASPERLAMGSFRDLLKRANVRTFAIDEAHCISHWGHDFRPEYRQLREL
                     RKTFPEASVHAYTATATPKVRQDIIEQLSLHDPIELVGDFDRPNLTYRVLPRTKEWEQ
                     VLDVVGRHQGEAGIIYCIRRRDVDSLSEKLRRKGVNAVAYHAGLSPEERRGAQEQFAT
                     EKCDLVVATVAFGMGIDRSNVRFVLHTGMPKSIEHYQQESGRAGRDGLEAECVMLYSA
                     ADAIVWRTMIERGGEGIEPDPVFVDAAMRHVRDLDRYCSAAACRHRSLVEYFGQQYTA
                     ANCNACDICLGETESVPDSLIIAQKILSCVYRVGQSFGVGHVTSVLRGEDTEKIRDRG
                     HARLSTFGLLKTSTKHELREWIYQLIAQDFLRQTEDEYPVLHLGARARDAMKGAVEVK
                     LRQPAARKKDKAARSTTANAPLEPLVAHDRELFESLRKWRRSEAEHRGVPPYVIFSDK
                     TLRELARMRPTTLHELRGIYGIGDAKLEAFGDALVTQIREHCATAPS"
     gene            complement(3535..4146)
                     /locus_tag="VNN25_08690"
     CDS             complement(3535..4146)
                     /locus_tag="VNN25_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91644.1"
                     /translation="MGHIEAAPKPTNPSVLDLFIEDARDWCRGRMWIPRAILLAYLVY
                     LFILKFRDPTRWTIFYGITLGFHEMGHLVTAWAPHFVTAIMGSVFQIAVPIVCIVYLA
                     RQPDYFGVSVGGCWLSYSLYELSAYVGDARAKDLPLVGFAASEDLEHDWGYILGALHM
                     LPADHFFAFLLRAAGLIAGVGSCAFAVWLLVTMAQSRSVRGSV"
     gene            complement(4191..4448)
                     /locus_tag="VNN25_08695"
     CDS             complement(4191..4448)
                     /locus_tag="VNN25_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXH91645.1"
                     /translation="MSSFAVETNPRATEVAVTPDEVIVHLADGRTVTVPLVWFPRLLH
                     ASQSERADFRLIGDGEYINWPSLDEDLSVAGLLRGARAVMS"
     gene            complement(4423..4662)
                     /locus_tag="VNN25_08700"
     CDS             complement(4423..4662)
                     /locus_tag="VNN25_08700"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXH91646.1"
                     /translation="MNFRRVGPYRLFFTSNDLDEPPHIHVERDGLVAKFWLSPVRLSK
                     SSRFAAHELSRMERIVRDNHSDIMEAWNEFLRRRN"
     gene            complement(4729..6141)
                     /locus_tag="VNN25_08705"
     CDS             complement(4729..6141)
                     /locus_tag="VNN25_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXH91647.1"
                     /translation="MPKLDPTSDEFRDAGHKLIDWIGDYLDGVDHYDVLSRVQPGDIA
                     KKFAAEPSRDGRPYEDLLADVESKIIPGITHWNHPAFLAYFANTASQAGILAELLTAA
                     LNANGMIWRTSPSLTELEMVTLGWLRDSLGLPNDLFGIINDTASINSFLALAAAREKS
                     APDIRTRGFSAAPALRVYCSEHAHSSIEKGALALGFGIDGVVKIAGDDALAMRIDVLE
                     EAIARDRKAGAIPCAIVATVGTTSSAAIDPVRRIAAIAQRENAWLHVDAAYAGPATIC
                     PEFRWLWDGVEGADSIVVNPHKWLFTPMDCSVLYTRQPEVLRETFSLVPEYLKTPDSA
                     EVNYMDYGLQLGRRFRALKLWFLFEHYGVARMQAVIRQHIADAGRLAEELQKRDDIEL
                     IAPPSLSVVVFRKVVRDSNGAIDEAASERASIVALERMNASGRLFVSHTRLRNRYGIR
                     VAIGNGATEWEHVSRVLEYL"
     gene            complement(6116..7459)
                     /locus_tag="VNN25_08710"
     CDS             complement(6116..7459)
                     /locus_tag="VNN25_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91648.1"
                     /translation="MTAALIAILSMTVLGLPITLAVDRRARGPLLVGASFLYGSGAIF
                     LILLALSVVKLRWTLPEVTLAALAVFCAASILARQPATGKAQPAARAHVFDVVTLFAL
                     TGYALYATLASLWEWDYWAIWGLKARVFLEAGGIDWRFLGSRWNTFAHTDYPLLVPLN
                     FDFVGLLNGSWSDRWLGLLCVAWGVAAVLIVRGLAARETSPFFASLIALALASRAVST
                     FIGLAEGALIAFGGAAVLFVRAALRDDDDAAAWRHGALMLGFAANCKNEGLALLVAVT
                     IAVAVVSWFRGGETSRSRRLATVTRLWPAYALAAPWLILRFTHVLPTDIAGGSPFSRL
                     MARLPHVHEILGFLITRLHDPWFWIVILAGTLIAPAAARRPERFVFLVTAIQLLFYVA
                     AYLATPNDLHWHVMTSWSRLPAQAALPITFVVFLMLANSLRGSQDAPHAQARPDE"
     gene            complement(7456..7962)
                     /locus_tag="VNN25_08715"
     CDS             complement(7456..7962)
                     /locus_tag="VNN25_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91649.1"
                     /translation="MLRRTLILIALLALLVVCIKPRMLQIVFTDREAAAREMNGSPDR
                     LWPLYPRFLEGVRAHTQPGDTIAIIAPGMAWDSGYSYAYYRASYFLTGREVLPLVTPE
                     NEKLPQNFRAARYIAAFEMTVRMPAEIVWQGEGGTLLRPVWGAVSPVGRANPSGGNAT
                     ILHPESQR"
     gene            complement(8363..9805)
                     /locus_tag="VNN25_08720"
     CDS             complement(8363..9805)
                     /locus_tag="VNN25_08720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXH91650.1"
                     /translation="MLKEKARMIARVVYVADLALTTAAFFAAFFVRDVVLPRVAPEHF
                     PTGLFPLRDYIKIYPVVLILWSVLLFTYHSYHSHRTIPLLREALTAIRVVFVGNAILA
                     TLAYLIPLRQLSRAWFVLFGVLAALLLVLEKIVVRIIARYVRAKGLNYRTILIVGTGR
                     RAIEVARTIEGHKYWGYKILGFVSDGHRLSNGWARFRVYGSVPDLRRLLEGGQFAEPI
                     DEIVFAVTRKKLDEMKQIFLMCEELGIRARVAMNFFPNRVARIELEELEGIPFLTFTT
                     TPSNESQLALKRLLDIAVSMLLLTLSLPAIAIAAVAIKLSSPGSVLFKQERMGLNGRL
                     FMLFKFRTMFEDAHERRGEVTHLNQMTGPVFKAKFDPRITAVGRVLRKFSLDELPQLW
                     NVLKGDMSLVGPRPPIPEEVAAYHRWHRRRLSMKPGLTCLWQVSGRNNVDFDRWMQLD
                     LQYIDNWSPSLDLKILLRTIPAVLSGRGAS"
     gene            complement(9903..>10381)
                     /locus_tag="VNN25_08725"
     CDS             complement(9903..>10381)
                     /locus_tag="VNN25_08725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH91651.1"
                     /translation="EVGMLRPPTPDPRPPLIMLGSASYARDLEAELHRHASERILFPG
                     ALFGVDYRTLQRHALLYIQATEVGGTHPAMIEAMGSGGCVLAHDTPENREVGGDAVGY
                     FKLRPSETLSGTIREYLDSPARRAQMRIAARARAAEKYGWTAVTDAYERLFSEIAR"
BASE COUNT         2095 a         3242 c         3305 g         1739 t
ORIGIN      
        1 ttcatccggt cgatgcgcgc ttccatctcg cggtcacgca gccaggtctt cgactccgac
       61 ttctccggca gctcctcgtc gggaatcgcc atcgcttcct tcaccaggcg aacgatgtcg
      121 cgtccgaagc ggccgcggtg atacccggag acacccttga tgctgttgaa atcctgcgtc
      181 gaagatggct tgatccgggc gatctcgaag atcagatcat tgccgaagat cttgaacggc
      241 gggcggtcgg cctcgcgggc cagaccgtcg cgaaaatcgt agagcgcgcg aacaacggcc
      301 agcgaacgac ggtcgagggc accgagcccc ttgagccggc ggaacggctc ctcgtcgcca
      361 tccttctcgt tgaatcggat ctgttcctgc ctgccaaact cctccaccgc ccactcccac
      421 cggcccagcg cggtcagctc atcgcggaga cgtccggcca gtgcgatcag atgccgcgta
      481 tcggtggccg cgtattcgag catgtcgggt ggcagcggac gcatcgccca gtcagcgcgt
      541 tgatgcttct tgtcgacctt gaaaccgaaa tggcggtcga gcagcgcggc cagaccaaag
      601 gcctcgtagc cgagtagctg cgagcagacc atcgtgtcga tcagattcgc gatcgtgaag
      661 ccgaagtcgc ggttgaggat gcgcaggtcg taatcggcgc cgtgcaggat cttcgtaatc
      721 tcgcgatttg ccaagatctc gccgaacttc gcgatatcga tcttgcacag aggatcgatg
      781 atcatgtcct cgccgggaat cgacatctgg atcaggcaga ccttgtcgaa atacgaatgc
      841 agcgaatcgg cctcggtgtc gagcgcgacg accggctgcg cggcaacgcg cgccatcgcg
      901 gcgtcgaagg ccacttgcgt atcgatgacg ttcacagcac acgctcccgc aacccgatcg
      961 ataccagctt ctcgttgaat gcactccggt cgaacgattc gccggcggca aggacacgca
     1021 catcgtccag ctcgtccgac atgctgagca gcagcgccag atcctcggcg gcaggaacgc
     1081 gaatctcccg ggggttggtc gcggtatgga gctcgatcgt acgcgcgttc gcgaacatgc
     1141 gcgcgtagag cgcgttgctg gccacgagta cgtgcacgcg aacatcgccc tcttcgtcga
     1201 cccacatcaa cggaacgagg tcgagctcct cggtctgctc gatctcgtcg cggtcgacgc
     1261 ggaagccgcg gttattcgcc atcatcgcaa cctgattctg ttgttgtggt tgcatcagtg
     1321 cgaggacgtc gatcgatccg ccgtcgatca tgccgccaag ccacgcaacg cgcgcaacgc
     1381 tgccgacgaa catcgactcg attcggagct tggagagaag ttctgcggtt gattcgattg
     1441 cccggacata gcgcgccgtc gcgcggagag gatgcatgag cgggcgatta tagttgctcg
     1501 ggagtcgcgg gtcgggagtc gggtgtcgga atgggagtgg ggagtagggt gtggggagtg
     1561 ggagtgaagc agaaagcttc gacacacacc gggcttcgag ctcccgacgc tccaggcttc
     1621 gatctccgac tcccgactcc cgactcccga ctcccgagtc ccgaaccccg ttccccactc
     1681 ccaccctcca ccccctactc cctacacccc acccccgcgg taccatgcct ccggtgctcg
     1741 atcgcctccg cgacctcatc tcccatcatt ggggatacga ttcgttccgt ccgttgcagc
     1801 gggaagccat gctcgccacg ctcgagcggc gcgactcgct tgtcgtgatg ccgaccggcg
     1861 gcggcaagtc gctctgctac caggctcccg cactgctcag cgagcacgtc acggtcgtcg
     1921 tctcgcctct tatctcgctg atgaaggatc aggtcgatgg cttgcgcgag tgtggcgtcg
     1981 ccgcgggcca gctcaacagc tcgcagcgca gcgaccagca gcgcgagatt gagcgcgccg
     2041 tgctcgacgg caagatcaag ctgctctttg catcgcctga gcgcctggcc atgggcagtt
     2101 ttcgcgattt gctcaaacgc gcgaacgttc ggacgttcgc gattgacgaa gcgcactgca
     2161 tcagccactg gggccacgat ttccgtcccg agtaccgcca gcttcgcgaa ttgcgcaaaa
     2221 cgttccccga ggcgtccgtg catgcgtaca cggccacagc gacgccgaaa gtgcggcagg
     2281 acatcatcga gcagctctcg ctccacgatc cgatcgagct cgtcggcgac ttcgaccggc
     2341 cgaacctcac ctatcgcgtt ctccctcgca cgaaagaatg ggagcaggtg ctcgacgtcg
     2401 tggggcggca tcaaggcgag gccggcatca tctactgcat ccgccggcgc gacgtcgatt
     2461 cgctctccga aaagctgcgg cgcaaagggg tcaacgccgt cgcttatcac gccggtctct
     2521 cacctgaaga gcggcgcgga gcgcaggaac agttcgcgac ggagaagtgc gacctggtcg
     2581 tcgccaccgt cgccttcgga atgggcatcg accgctccaa cgtccgcttc gtgctccaca
     2641 ccggcatgcc gaaatcgatc gagcactacc agcaggagag cggtcgagcc gggcgcgatg
     2701 gcctcgaagc cgagtgcgtg atgctctact ccgccgccga tgccatcgtc tggcggacga
     2761 tgatcgagcg cggaggcgaa ggcatcgagc ccgatccggt cttcgtcgat gcggcgatgc
     2821 gtcatgtgcg cgatctcgat cgctactgtt ccgcggccgc ctgccggcat cgctcgctgg
     2881 tggagtattt cggccagcag tacacagcgg caaactgcaa cgcctgcgac atctgcctcg
     2941 gtgaaacgga gagcgtcccc gactcgctga tcatcgcgca gaagattctc tcgtgtgtct
     3001 atcgcgtcgg ccagtcattc ggcgtcgggc acgtcacgag cgtcctgcgc ggcgaagaca
     3061 cggagaagat ccgcgaccgt ggccacgcac ggctgtcgac gttcgggctg ctcaagacgt
     3121 cgacgaagca cgaactgcgc gaatggatct accagctcat cgcgcaggac ttccttcgcc
     3181 agactgagga cgaatacccg gtcctccacc tcggcgcccg tgctcgcgac gcgatgaaag
     3241 gcgctgtcga ggtgaagctg cggcagccgg cggcgcgaaa gaaggacaaa gcggcgcgct
     3301 ccaccacggc aaacgctccg ctcgagccgc tcgttgcgca cgaccgcgag cttttcgagt
     3361 cgctgcgcaa atggcgccgc tcggaggccg agcaccgagg cgtgccgccc tacgtcatct
     3421 tcagcgacaa gaccctgcgc gagcttgcgc ggatgcgccc gacgacgctt cacgagctgc
     3481 gcgggatcta tggcatcggc gacgcgaaac tcgaggcgtt cggcgacgcg ctcgtcacac
     3541 agatccgcga acactgcgcg actgcgccat cgtaacgagc agccacaccg caaacgcgca
     3601 cgatcccact ccggcgatca atcctgccgc ccgcagcaga aacgcgaaga aatgatcggc
     3661 tggcagcatg tgcagcgcgc cgaggatgta tccccagtcg tgttccagat cctcggaggc
     3721 cgcgaatccg accagcggca aatccttcgc tcgcgcgtca cccacatacg cggaaagctc
     3781 gtacaggctg tacgaaagcc agcagccgcc gaccgagact ccgaaatagt ccggctgccg
     3841 cgccaggtag acgatgcaga caatcggcac ggcaatctga aacacgctcc ccatgatcgc
     3901 agtgacgaag tggggcgccc aggcggtgac gagatgcccc atctcgtgga agccaagggt
     3961 gatgccgtag aagatcgtcc agcgagtggg gtcgcgaaac ttgaggatga acaggtagac
     4021 cagataggcg agcaggatcg cccgcggaat ccacatccgc ccgcggcacc agtcgcgcgc
     4081 gtcttcgatg aaaagatcga ggactgaggg attggttggt ttgggtgcgg cttcgatgtg
     4141 acccattggg ggaagtctaa accgtccgtg gatgggcaac tgcgaagcag ctacgacatg
     4201 actgcacgcg cgcctcgcaa caatccggcg acgctcagat cctcatcaag agatggccag
     4261 ttgatgtatt cgccatctcc aatgaggcga aaatccgccc gctcgctttg agacgcatgc
     4321 agcaagcgcg gaaaccacac gagcggaacc gtaacggtgc gcccatccgc aaggtggacg
     4381 atcacttcgt caggcgttac agctacttcg gtcgcccttg gattagtttc gacggcgaag
     4441 gaactcattc caagcctcca tgatgtcgga gtggttatcg cgcacgattc gttccatcct
     4501 cgacaactca tgcgccgcga aacgcgatga cttcgataag cgaaccggcg acaaccagaa
     4561 ctttgctacc aatccatctc gctccacatg aatgtgaggt ggctcatcaa gatcgttgct
     4621 cgtgaaaaac agccgataag gccctacgcg cctgaagttc acaatcgtct cccgtgaact
     4681 acccggacag aataccggaa caatcgtgtc cgggtcgttc cgcgaagatc acaaatactc
     4741 gagtacccgc gaaacatgct cccactcggt cgcgccgttg ccgatggcga cgcggatgcc
     4801 gtagcggttg cggaggcggg tgtgggaaac gaagaggcgg ccgctggcgt tcattcgttc
     4861 gagcgcgacg atgctggcgc gctcagatgc tgcttcatcg atagcaccgt tggaatcgcg
     4921 gacgactttg cggaagacga cgacgctgag gctcggcggc gcgatcaact cgatgtcgtc
     4981 gcgtttctgc agctcctcgg ccagacgtcc ggcatcggcg atgtgctgac ggatcaccgc
     5041 ctgcatccgc gcaacgccgt agtgctcgaa caggaaccag agtttgagcg cgcggaagcg
     5101 gcggccgagc tggaggccgt agtccatgta gttcacttcg gccgaatcgg gcgtcttcag
     5161 gtattcaggc accagcgaga acgtctcgcg cagtacctcg ggctggcgcg tgtagagcac
     5221 gctgcaatcc atcggcgtga agagccactt gtgcgggttg acgacgatcg agtcagcacc
     5281 ttcgacgccg tcccagagcc agcgaaactc ggggcagatc gtggcggggc cggcgtatgc
     5341 ggcgtcaacg tgaagccacg cgttctcgcg ttgcgcgatg gccgcaatcc tgcgaacggg
     5401 atcgatcgcc gccgacgacg ttgtgccgac cgtagcgacg atcgcacagg ggatggcgcc
     5461 ggccttgcga tcacgcgcga tcgcttcttc gagcacatca atccgcatcg ccagcgcatc
     5521 gtcgccagcg atcttgacca caccgtcgat gccgaatccg agggcgagcg ctcccttctc
     5581 gatcgaggag tgagcgtgtt ccgagcagta aacgcgcaac gccggcgcgg cggaaaagcc
     5641 gcgtgtgcga atgtccggtg ccgatttctc gcgggcagcg gcaagcgcca ggaacgagtt
     5701 gattgacgcc gtgtcgttga tgatgccgaa gagatcgttc ggaaggccga gggagtcgcg
     5761 aagccaaccg agcgtaacca tctccagctc ggtcagagag ggagacgtgc gccagatcat
     5821 cccgttcgcg ttcagcgcag ccgtcagaag ctcggccagg attccggcct gcgaggcggt
     5881 attggcgaag taggcgagga acgcgggatg gttccagtgg gtgatgccgg ggatgatctt
     5941 cgactcgacg tcggcaagga gatcctcgta tggacgtccg tcgcggctcg gctccgcggc
     6001 aaacttcttc gcgatgtcgc ccggctgcac gcgcgagagc acgtcgtagt gatcgacgcc
     6061 gtcgagatag tcgccgatcc agtcgatcag cttgtggccg gcgtcgcgga actcatcact
     6121 cgtcgggtcg agcttgggca tgcggcgcat cttggctgcc ccgcaacgaa ttcgcaagca
     6181 tcagaaagac cacgaaggtg atgggcaggg ccgcctgtgc cggcagccgc gaccacgaag
     6241 tcatcacatg ccagtgcagg tcgttcggcg tcgccagata cgcagcgaca taaaagagaa
     6301 gctggatggc cgtgaccagg aagacgaagc gctcgggtcg ccgggccgcg gccggagcga
     6361 tcagagtccc ggccaggatc acgatccaga accacgggtc atgcagccgt gtgatgagaa
     6421 agccgagaat ctcgtggacg tgcggcaggc gcgccatgag acgagagaac ggagacccgc
     6481 cggcgatgtc ggtcggcagc acatgagtga aacggagaat gagccacggc gcggcgagcg
     6541 cgtatgcggg ccagagtctt gtcacggtcg caaggcgacg cgaacgcgat gtctcgccgc
     6601 ctcgaaacca tgagacgacg gcaaccgcga tcgtcactgc cacgagcagt gccagacctt
     6661 cgttcttgca gttcgcggcg aagccgagca tgagcgctcc gtggcgccac gctgcggcat
     6721 cgtcgtcgtc gcgaagggca gcacgcacga agagaacggc cgcgcctcca aacgcgatga
     6781 gcgccccttc cgcgagtccg atgaacgtgc tgacggcgcg cgacgcgagg gcgagcgcga
     6841 tgagagacgc gaagaatggc gaggtctcgc gcgcggccag cccgcgcacg atcagcaccg
     6901 cagcgacgcc ccaggccaca cagagcagcc cgagccagcg atcgctccag ctaccgttga
     6961 gcaggccgac aaagtcgaaa ttcagcggga cgagaagcgg atagtcggta tgggcgaacg
     7021 tgttccagcg gctgccgagg aaacgccaat cgatccctcc cgcttcgagg aagacgcgcg
     7081 ccttgaggcc ccagatcgcc cagtaatccc actcccagag tgacgccagc gtggcgtaga
     7141 gcgcgtagcc ggtcagcgca aacagcgtaa ccacgtcgaa gacatgcgca cgcgcagcag
     7201 gttgcgcctt gccggttgcc ggctgtcttg ccaggatcga cgccgcgcag aagacggcca
     7261 gggcagcgag cgtaacctcg ggcagcgtcc agcgcagctt cacgaccgaa agggcgagca
     7321 ggatgagaaa gatggccccg ctgccataaa ggaacgacgc cccgaccagc agcggtcctc
     7381 gggcgcggcg atcgaccgca agcgtgatcg gcagcccgag gaccgtcatg ctgaggatgg
     7441 cgatgagcgc ggcggtcatc gctgcgattc cgggtggagt atggtggcgt taccgccgga
     7501 aggattcgcc cgccccacag gcgacaccgc gccccagacc ggccgcagaa gagtgccccc
     7561 ctcgccctgc cagacgatct cggcaggcat tcggaccgtc atttcaaacg cggcgatgta
     7621 gcgtgcggcg cggaagtttt gcggaagctt ctcgttctca ggagtgacga gcggcagtac
     7681 ctcgcggccg gtgagaaagt aactggcacg gtagtaggcg tacgagtagc cgctgtccca
     7741 cgccatgccg ggggcgatga tggcgatcgt gtcgccgggc tgggtatggg cgcgaacgcc
     7801 ctcgagaaag cgcggataga gcggccacag ccggtccgga gagccattca tctcgcgtgc
     7861 ggctgcctcg cgatcggtga acacgatctg cagcatgcgc ggtttgatgc acaccaccag
     7921 caaggcaagc agagcaatca ggatgagtgt gcggcgcagc atgtgacgcg attgtacggg
     7981 gttggggggt ggggagtcgg gggtggggcg tggtcagtct cttacgaagg actcccgagt
     8041 cttatgcgtg aaaccccgag tctcatgcgt gatacccccg agtctcatgc gtgatacccc
     8101 cgagtctcat gcgtgatact ccgagtctca tgcgtgatac tccgagtctc atgcgtgata
     8161 ctccgagtct catgcatgag actctgagtc tcacgcgtta gatagagata gatgagctaa
     8221 acgctttcag gctcagtaac ttaccagcct tggatcaaga aagaggctcc ggcgagctcg
     8281 agcaatggcg aggcgaactg ttttgcaaag ctgatcttgg agattcacaa cccgaccggc
     8341 gttcccgcgg ccgattctgc ccttagctgg cgccgcgtcc cgaaagaacc gcgggaatcg
     8401 tccgcagcag gatcttcaga tccaacgatg gcgaccagtt gtcgatgtac tgcaggtcga
     8461 gctgcatcca gcggtcgaaa tcgacgttgt tcctgccgga cacctgccac aaacacgtga
     8521 ggcccggctt catcgacaga cggcggcggt gccagcggtg ataggcggcc acttcctcgg
     8581 gaatcggcgg gcgcggaccg accaggctca tgtcgccctt gagcacgttc cagagctggg
     8641 gcagctcgtc gagcgagaac ttccgcagga cacggccgac ggcggtgatg cgtggatcga
     8701 acttggcttt gaaaaccggc ccggtcatct gattcaagtg cgtgacttcg ccgcgccgct
     8761 cgtgcgcatc ctcgaacatc gtccggaact tgaagagcat gaagagtcgt ccgttgagac
     8821 ccatccgctc ctgcttgaag aggaccgagc ccggagagga gagcttgatg gccacggccg
     8881 cgatggcgat cgcaggaagc gacagcgtaa gaagaagcat cgagacggcg atgtcgagaa
     8941 ggcgcttgag ggcgagctgc gactcgttcg agggcgtcgt cgtgaacgtg aggaacggaa
     9001 tcccctccag ctcctccaac tcgatgcggg ccacccggtt cgggaagaag ttcatcgcca
     9061 cgcgggcgcg gataccgagc tcttcgcaca tcaggaagat ctgcttcatc tcgtccagct
     9121 tcttgcgcgt gacggcgaac acgatctcgt cgatcggctc ggcgaactga ccaccctcga
     9181 gcaggcggcg cagatcgggg acgctgccat agacacggaa gcgcgcccaa ccgttcgata
     9241 ggcggtgccc gtcggagacg aagccgagga tcttgtagcc ccagtatttg tggccttcga
     9301 tcgtgcgcgc cacttcgatg gccctccggc ccgtgccgac gatgagaatc gtccggtagt
     9361 tgaggccttt cgctcgaaca tagcgggcga tgatcctcac cacgatcttc tcgaggacca
     9421 gcagcagcgc tgccagcacg ccgaagagga cgaaccacgc acgcgacaac tggcggagcg
     9481 gaatgaggta ggccagggtg gccaggatag cgttgccgac gaacaccacg cgaatggcgg
     9541 tcagcgcctc gcgcaggagc gggatcgtgc ggtggctgtg atagctgtgg tacgtgaaga
     9601 gaagaaccga ccagaggatc aggacgaccg gatagatctt gatgtagtcg cgaaggggga
     9661 aaaggccggt aggaaagtgc tccggagcca cgcgcggcag cacgacgtca cgcacgaaga
     9721 acgctgcaaa aaaagcagcc gtcgtgaggg ccaggtcggc gacatagacg accctcgcga
     9781 tcatgcgcgc tttctctttg agcatctaac ctcttgcctc agcgaacgat ggcttctcga
     9841 atgtaacgca ctactggctg accggtatac ggaaatcgcg gcgagcgcgt ctacggcatt
     9901 cttcagcggg cgatttccga aaaaagccgc tcgtacgcgt cggtcacagc cgtccagcca
     9961 tacttctcag ccgcccgagc ccgcgcggca atcctcatct gcgcccgcct ggccggcgaa
    10021 tcgagatatt cgcgaattgt gcctgaaagc gtctcggacg gacgcagctt gaagtaaccg
    10081 accgcgtccc cgccaacttc ccggttctcc ggcgtgtcat gcgccagcac acagccaccc
    10141 gaccccatcg cctcgatcat cgccggatgc gtccccccca cctcggtcgc ctgaatgtag
    10201 aggagagcat ggcgctgaag agtccgatag tcgaccccga aaagcgcacc cggaaagagg
    10261 attcgctcgg atgcatggcg gtgaagctcc gcttcgaggt cgcgggcgta cgaggcggag
    10321 ccgagcatga taagcggggg tcgcgggtcg ggggtcgggg gtcggagcat tccgacttcc
    10381 g
//
LOCUS       DATLVW010000282         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_463629, whole genome shotgun sequence.
ACCESSION   DATLVW010000282 DATLVW010000000
VERSION     DATLVW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463629"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VNN25_08730"
     CDS             complement(<1..555)
                     /locus_tag="VNN25_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91652.1"
                     /translation="MFFENVKIALRAIGANKMRSILTVLGVMIGVAAVIAVVSLVQGM
                     QFNISNQLENVGATFIRVVPDFSASRGNPFLPVPQLTFDDAMAVRRGATAIRDFTPLF
                     ISNASLKNGDSTHDTTLFAVNQSYPDVAKQFVDHGRFFSTLDEDAKKRVCVLGSEAAR
                     QLGFVDAIGKDIRVDSNNFTVVGVM"
     gene            complement(583..1236)
                     /locus_tag="VNN25_08735"
     CDS             complement(583..1236)
                     /locus_tag="VNN25_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91653.1"
                     /translation="MRELTRTYQLGPQDIFALRGVDLIINHGEYVAIMGPSGSGKSTL
                     MNIVGCLDRPSGGQYILDGIPVESMDDDALAAVRNKKIGFVFQTFNLLARTTALQNVE
                     LPLVYAKIGRHERRDMAEEALTAVGLGDRMNHQPNELSGGQRQRVAIARALVNKPSLL
                     LADEPTGNLDSQTGREILDLFRELSSRGNSIIMVTHEDDVAQEAQRVIHLRDGKITD"
     gene            complement(1269..2513)
                     /locus_tag="VNN25_08740"
     CDS             complement(1269..2513)
                     /locus_tag="VNN25_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH91654.1"
                     /translation="MKKPLIIIGALVLVGAIIFASVRGGGSKGEPVYVEPVTARNIQA
                     IVTAPGEIDPKVSVNISAHIVGKIEKIYFAEGDTVHKGQRLVDLEKTAYQAAYLRSGA
                     AVTSSRIEVARAKTAVSTAETAYHRAVNLKQQGIQAQDLFDQARQANDNAVAAYDSAK
                     QGVAQAEAMLQQAQTDLDHTTILSPIDGKAVQLNAHEGEVVITGTMNNPGSVIAVIAD
                     LSEILVEAQVGETEITGIRLNEPAKVHVDAIPDKEYAGHVAEIGSSAALKQGAGSGMR
                     YFKVKVQVDNPDDRLRPGMTSQVSIVTTSAGSALSVPIQSVVERVPPSLAKSPTGTDD
                     EDEKAPKLKYVFVVKDGKVKMTEVKTGVSDATHVVLTSGVTANVKVVSGPFRTLKKLK
                     DGDSVEVTKEESKTPQNGTASK"
     gene            complement(2510..>2853)
                     /locus_tag="VNN25_08745"
     CDS             complement(2510..>2853)
                     /locus_tag="VNN25_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91655.1"
                     /translation="VTCYSSMPGVIKSVVLMIIIVAKGGIIPAPQLATLVRSNLGFLV
                     DLKANPMAFAVLSSIDIFSIWFLALMIIGFAYLARVSRAKSAVVIISLWLVVLCFKLI
                     GPALQSVRANK"
BASE COUNT          518 a          818 c          930 g          587 t
ORIGIN      
        1 catcacgccg acgacggtga agttgttgct atcgacgcgg atgtctttgc cgatggcgtc
       61 gacgaatccg agctggcggg cggcttcgga gccgagaacg cagacccgct ttttggcgtc
      121 ttcgtccagc gtgctgaaga agcggccatg gtcgacgaat tgtttggcga cgtcggggta
      181 gctctggttg acggcgaaga gcgtggtgtc gtgggtcgaa tcgccgtttt tcagcgacgc
      241 gttgctgatg aagaggggcg tgaagtcgcg aatggccgtg gcgccgcggc gcacggccat
      301 ggcatcgtcg aatgtgagct gcggcactgg caggaacgga ttccctcggg atgccgagaa
      361 atcgggaacg acgcgaatga aggtcgcgcc gacgttctcg agctgattcg agatgttgaa
      421 ctgcatcccc tgcacgagcg aaacgacggc gatgaccgcg gccacgccga tcatcacacc
      481 gaggacggtg aggatcgagc gcatcttgtt ggcgccgatg gcgcggagtg cgatcttgac
      541 gttttcgaaa aacatcgtgc ttgagtctag atgatcaaag gcctagtcgg tgatcttgcc
      601 gtcgcggagg tgaatgacgc gttgcgcttc ctgcgcgaca tcgtcctcgt gcgtgaccat
      661 gatgatcgag ttgccgcgcg aggagagctc gcggaaaagg tcgaggatct cgcgtccggt
      721 ctgggagtcg aggttaccgg tcggctcgtc ggcgaggagc agcgatggct tgttgacgag
      781 cgcccgggca atggcgacgc gctgccgctg accgccggag agctcgttcg gctggtggtt
      841 catgcgatcg ccgaggccga ccgcggtaag tgcctcttcg gccatgtcgc ggcgctcgtg
      901 gcggccgatc ttcgcgtaga ccagcggcaa ctcgacgttc tggagcgccg tggttcgtgc
      961 aagcagattg aaggtctgga aaacgaatcc gatcttcttg ttgcgcacgg ctgccagtgc
     1021 gtcgtcgtcc atcgattcga caggaatccc gtcgagaatg tactggccgc cggacggacg
     1081 gtccaggcag ccgacgatgt tcatgagcgt cgatttgccg gagcccgacg gtcccatgat
     1141 ggcgacgtac tcgccgtggt tgatgatcag atcgacgccg cgaagagcga agatgtcctg
     1201 cggtccgagc tggtaggtgc gcgtgagctc gcgcatctcg atcaggtgct ttccgtttcc
     1261 gttctcagtc actttgaggc cgtcccgttt tgaggagtct tcgactcttc tttcgtcact
     1321 tctacggaat cgccgtcttt cagtttcttc agagtgcgga aaggtccgga aacgaccttc
     1381 acattcgccg tgacacccga ggtgagaacg acatgcgtgg catcgctcac acccgtcttg
     1441 acctcggtca tcttgacttt gccgtccttc accacgaaga cgtacttgag ctttggcgcc
     1501 ttctcgtcct cgtcatcggt accggtcggt gacttcgcca acgacggagg aacacgctcg
     1561 acgacgctct gaatcggaac ggacaacgcg ctgcccgcgc tggttgtgac gatggagact
     1621 tgcgacgtca tcccggggcg tagccggtca tcaggattgt cgacttgaac cttgactttg
     1681 aagtagcgca tgccggagcc ggcaccctgc ttcagtgccg cggagctgcc gatctcggcg
     1741 acgtgaccgg cgtactcctt gtccggaatg gcatcgacgt gaactttggc cggctcgttg
     1801 aggcgaatgc cggtgatctc agtctccccc acctgcgcct cgacgagaat ctcggaaagg
     1861 tcggcgatga cggcgatgac ggaaccggga ttgttcatcg tgccggtgat gacgacttcg
     1921 ccctcatgcg cgttgagctg cacggccttg ccgtcgatgg gcgacagaat ggtggtgtga
     1981 tcgaggtcgg tctgggcctg ctggagcatc gcttccgcct gcgccacgcc ctgcttcgcg
     2041 gagtcgtatg ccgccacggc gttgtcattc gcctgccggg cctgatcgaa gaggtcctgc
     2101 gcctggatgc cctgctgttt gagattcacg gcgcggtggt acgcggtctc ggcggtagac
     2161 acggctgttt tcgcgcgcgc gacttcgatt ctgctgctgg tgaccgcggc acccgaacgc
     2221 aggtaggcgg cttgatacgc ggtcttctcc agatcgacaa ggcgttgccc tttgtgaacc
     2281 gtgtcgccct cggcgaagta gatcttctcg atcttgccga cgatgtgcgc gctgatgttg
     2341 acggaaactt tcggatcgat ctcacccggt gccgtgacga tcgcctggat gttgcgcgcc
     2401 gtcaccggct cgacatagac aggctcgcct ttggaaccac cgccgcgaac gctcgcaaag
     2461 atgatcgcgc caacgaggac gagcgcaccg atgatgatga gcggcttctt catttattgg
     2521 cacgcactga ctgcaacgca gggccgatca gcttgaagca gagcacgacg agccagaggc
     2581 tgatgatgac gacggccgat ttggcgcgcg acacgcgcgc cagatacgcg aacccgatga
     2641 tcatgagcgc gaggaaccag atactgaaaa tgtcgatcga agagagcacc gcgaaggcca
     2701 tcggattggc tttgagatcg acgagaaacc ccaggttcga gcggaccagc gtcgctaact
     2761 gcggcgcggg gatgatgccg cccttggcca cgatgatgat catcaggacg acgctcttga
     2821 tcacgcccgg catcgacgaa tagcaggtca ccg
//
LOCUS       DATLVW010000283         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_11177, whole genome shotgun sequence.
ACCESSION   DATLVW010000283 DATLVW010000000
VERSION     DATLVW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11177"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..524)
                     /gene="queF"
                     /locus_tag="VNN25_08750"
     CDS             complement(171..524)
                     /gene="queF"
                     /locus_tag="VNN25_08750"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXH91656.1"
                     /translation="MPTVPNRELQTFANPNPERDYEIAFEAPEFTCLCPMTGQPDFAT
                     IRIRYVPDAKCVELKSLKLYLWSYRDEGTFHEAVTNKIANDLVAALDPRFLYVEGDFF
                     VRGGIKTVVKVEHRK"
     gene            complement(555..1784)
                     /locus_tag="VNN25_08755"
     CDS             complement(555..1784)
                     /locus_tag="VNN25_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXH91657.1"
                     /translation="MRRLLLTALLLIATPLSADIRGTVSIDLRNARLNATLDDDYTAV
                     AEESSVKLLLHPALEVHKVTCPICGAFKVERNGDDPATLQIDLTRPLAKGERVVLHIE
                     YGGSIAASYQRSEEFLELGLDDFWYPVNPRIGEIDFRYRLRVHVDEGGFQLVSNGVVK
                     KTADGWLVTSRVPDIDIDLVLGHHLDTVREARNGYDLAIVTQNLAPAVPKKLLGDMRR
                     VLDFYDSTFGASSPERAVTGVFRPHVSRDGQGGYFRKGYFVLPKLVDPGATLPNIAHE
                     LAHHWWIHASQQNAWLNESFAEYSAMMAMRKLRGVEAFRKIVAEKASRVSAAPLPPIY
                     GFDRTKNRRITPLMMYAKGPLVLNALEREIGEAKFIHLLRRMVTGNVVDPDHLVVLIG
                     EVASPRVAADFLQLLKE"
     gene            complement(1798..>2802)
                     /locus_tag="VNN25_08760"
     CDS             complement(1798..>2802)
                     /locus_tag="VNN25_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXH91658.1"
                     /translation="LAAMISHITETEPLHIVTIEDPIEFLFIDKIAAISQREVGSDTP
                     NFREALRNAMRQDPDVIMVGEMRDVETVETVLTAAETGHLVFSTLHTNNAAQTIDRII
                     DTFPADQHKQIRSQLALVLRGIISLKLIKTTTGTLTAAVEILKNSPRIAKLIEDGHTK
                     DILDEMESSVGLYRMQSMNQSLIALLVHQKISYQDAMALSSDSDDLSLKVRKLFPQIE
                     ERVRQGGEMAPSYSDFSQITELMDIKRLYEEQEVNWRQRVATKEEEVEDLRHDMEVLR
                     QQMQQGSASRSQHDEEIARMRADNERLRADAQAKITQLQERIKELNQKLMASGPMAKK
                     "
BASE COUNT          513 a          834 c          877 g          578 t
ORIGIN      
        1 tgccgccttc ttcgccggat cgagaagctt gaagaggggc ggcggaaata gagcctcacc
       61 actctgagcg ttgtggagct ccgacgccgg ataaccgggg atgaagatga cgggtttgcg
      121 agccattgca agacctccat ccaataagac gtcctcgaac cagcttttcc ctacttccgg
      181 tgctcgacct tcacgaccgt cttgatccct ccgcgcacga aaaagtcgcc ttcgacgtag
      241 agaaatcgtg gatcgagggc ggcgacgaga tcatttgcga tcttgttcgt caccgcctcg
      301 tggaacgtcc cttcatcgcg gtacgaccag agatagagct tcagcgattt gagctcgacg
      361 cacttcgcgt cagggacgta gcggatgcgg atcgttgcga agtcgggctg cccggtcatc
      421 gggcagagac aggtgaactc gggcgcctcg aacgcgatct cgtagtcgcg ctcggggttc
      481 gggttggcga acgtctgcag ctctcgattc ggaacggttg gcatcgcgag cggaaaaacg
      541 agtcacatgc cgattcattc cttcagcaat tggaggaagt cggcggccac gcgcggcgac
      601 gcgacctcgc cgatcaacac cacgagatga tcgggatcga ccacgttacc ggtcaccatg
      661 cgtcgaagga ggtggatgaa cttcgcttcc ccgatctccc gctccaacgc gtttaaaacc
      721 agagggcctt tcgcatacat catcaacggc gtgatgcgcc ggttcttcgt ccgatcgaat
      781 ccgtagatcg gcggcagcgg cgcggccgac acccgcgacg ccttctccgc cacgatcttc
      841 cggaacgctt cgacgccgcg cagtttgcgc attgccatca tcgcgctgta ttcagcgaag
      901 ctttcattca gccaggcgtt ctgctggctg gcgtggatcc accagtgatg cgccagttcg
      961 tgagcgatgt tcggaagtgt cgcgccggga tcgacgagct tcggcagtac gaagtagccc
     1021 ttgcggaagt agccgccctg cccgtcgcgc gaaacgtgcg gacgaaaaac accggtgacg
     1081 gcacgctcgg gactcgaagc tccgaacgtc gagtcgtaga aatcgagcac gcggcgcata
     1141 tcgccgagga gcttcttcgg caccgcggga gcgaggttct gcgtgacgat ggcgaggtcg
     1201 taaccgttgc gcgcctcgcg aaccgtatcg aggtgatggc cgaggacgag atcgatgtcg
     1261 atgtcgggaa cgcgcgaagt cacgagccag ccatccgccg tcttcttcac gacgccgttg
     1321 ctcacgagct ggaatccccc ctcatcgaca tggacgcgca gccggtagcg aaaatcgatc
     1381 tcgccgatgc gcggatttac cggataccag aagtcgtcga gaccgagctc gagaaactcc
     1441 tcgcttcgct ggtacgacgc cgcgatgctt ccgccgtatt cgatgtgcaa cacgacgcgc
     1501 tcgcccttcg ccagcggcct ggtcaaatcg atctgcaacg tcgccgggtc gtcaccgttg
     1561 cgctcgactt tgaaagcgcc gcagatcggg caggtgactt tgtgcacttc aagtgccgga
     1621 tgcaggagca gcttgaccga cgactcttcg gcgaccgcgg tgtaatcgtc atcgagtgtc
     1681 gcgttgagcc gcgcgttgcg gaggtcgatc gacacggtgc cgcggatatc ggcggacagc
     1741 ggcgtcgcaa tcaggagaag cgccgtgagc agaagccttc tcatgacgaa ctgaaggtca
     1801 cttcttcgcc attggccccg acgccatcaa cttctgattc agctccttga tgcgttcctg
     1861 gagctgcgtg atcttggcct gggcgtcggc gcggaggcgt tcgttatcgg cgcgcatccg
     1921 ggcgatctct tcgtcgtgtt gcgagcgcga agcggagccc tgctgcatct gctggcgcag
     1981 aacctccatg tcgtggcgca ggtcttcgac ctcttcttcc ttcgtggcga cgcgctggcg
     2041 ccagttcact tcctgctctt cgtagagccg cttgatgtcc atcagctcgg tgatctgcga
     2101 gaaatcggaa tagctcggag ccatctctcc cccctggcgg acgcgctctt cgatctgcgg
     2161 gaagagcttg cgcactttga gcgacaggtc gtcggagtcg ctcgaaaggg ccatggcgtc
     2221 ctgataggaa atcttctgat gcacgagcag cgcgatgagc gactggttca tcgactgcat
     2281 tctgtagagc ccgaccgagc tctccatctc gtcgaggatg tctttggtgt ggccgtcttc
     2341 gatcaacttg gcgatgcgcg gcgagttctt gagaatctcc acggcagcgg tgagcgtgcc
     2401 ggtcgtcgtc ttgatgagct tcagcgaaat gatcccgcga aggacgaggg cgagctgcga
     2461 gcggatctgt ttgtgctgat cggccgggaa ggtgtcgatg atgcggtcga tggtctgcgc
     2521 ggcgttgttc gtgtgaagcg tggagaagac caggtgcccg gtctcggccg cggtcagcac
     2581 cgtctcgacc gtttcgacgt cccgcatctc gccgaccatg atgacgtccg ggtcctggcg
     2641 catggcgttg cgcaacgctt cccggaagtt cggcgtatcg ctgccgactt cgcgctgtga
     2701 gatcgccgcg atcttgtcga tgaacagaaa ctcgatcgga tcttcgatcg tgacgatatg
     2761 cagcggttcg gtctcggtga tgtgcgagat catcgctgcc ag
//
LOCUS       DATLVW010000284        11214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_590083, whole genome shotgun sequence.
ACCESSION   DATLVW010000284 DATLVW010000000
VERSION     DATLVW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590083"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1120
                     /locus_tag="VNN25_08765"
     CDS             395..1120
                     /locus_tag="VNN25_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91659.1"
                     /translation="MSTKKRTGNRSSHPVVQKRSRTKPKAIAAIPDLVAAAARMVELL
                     NEMEAVIPDLTQPDIAKIPRMAQSAKFGRALIPPTINAVNSYPPFQQRNMFDAAAAQA
                     ALDYQGQMQPITKRMAAIVMAMSYSMNQKMSVSGEQALQLFQYGKRHVKQPDGGGARI
                     YVDEMQSVVDRTMNRRKPRKPATNPPTTPPSNTPPTTPTMQSFMAPSLAAKARTKAQI
                     VPDIDPDDDEIRAIIQGIATDPE"
     gene            complement(1184..2548)
                     /locus_tag="VNN25_08770"
     CDS             complement(1184..2548)
                     /locus_tag="VNN25_08770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH91660.1"
                     /translation="MPFEALGLDPKIAKAVREAGYTEPTPIQTKAIPLVLRGHDVIGI
                     AQTGTGKTAAFVLPILQRLSTRGSATNRRCMRAIVIAPTRELVVQIEENVRKYARHLH
                     LRIATVYGGVGERPQIQALRAGTEIVIATPGRLIDLMDQGHVDFSGLEVAVLDEADRM
                     LDMGFLPAMRRIVKALPKQRQTMMFSATLSKEIETLTNEFLSDSQLVQVGKRANPAET
                     VTQFVYETTKEAKLPLLLHLLRDPSLDSVLVFSRTKHGADKIARKLASSGINTGTLHS
                     NRSQSQRIAALKAFKAGTVRVLVATDIAARGIDVEGISHVVNFDFPPQPEDYVHRIGR
                     TGRAKAIGDAISFVTPDDRGNLRDLERFISRGIPRKVAEGFDANAYKTAEPLKSWRPS
                     RPAHAPRPQRANTYTSSRPTARPGSGRPSAARPSSARPHAGGNGRAASSDRPQFSDRA
                     RRRP"
     gene            complement(2671..2889)
                     /locus_tag="VNN25_08775"
     CDS             complement(2671..2889)
                     /locus_tag="VNN25_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91661.1"
                     /translation="MNQDNDRDLEIAEQTAAPEGVRGKYYARYREDTNLVLLAPDVAE
                     VFRDATSVNAALRQFLAEHGAPPAKTTG"
     gene            complement(2876..3148)
                     /locus_tag="VNN25_08780"
     CDS             complement(2876..3148)
                     /locus_tag="VNN25_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXH91662.1"
                     /translation="MKYEWDPEKDRRNKSKHGISFEEAATVFGDDFALTWQDREHYEQ
                     EYRMLTLGYTNTHRLVIVAHTEREDRTRIITAREATAGERRLYESG"
     gene            complement(3186..4049)
                     /locus_tag="VNN25_08785"
     CDS             complement(3186..4049)
                     /locus_tag="VNN25_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXH91663.1"
                     /translation="MFTIVYLLFAALALGAPLPHVALQPRGTTLRIAVVGDTGEGSDR
                     VARGIAKLHAKTPLDAVIITGDAFYPCGPAGKTDPQWSRVRALGQIGVPIFPVLGNHD
                     FCGKSVPDAQIGTAPVAHWQFPARQYVVDARIAELLMLDTTPFAVARSDAAADALRQT
                     LSSKKTSWRIAVGHHPIVSSGYHGHFPRAQHVRMVTLEPVMKRARVDLYICGHDHHLE
                     LIDGQPRYLISGAGSDPVPPIVPHAKTLFPSDATTQLGFAVLELDDHSMTIQFFDGEG
                     TAISKAFTFRR"
     gene            4184..4281
                     /gene="ffs"
                     /locus_tag="VNN25_08790"
     ncRNA           4184..4281
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNN25_08790"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4356..5903
                     /gene="dnaX"
                     /locus_tag="VNN25_08795"
     CDS             4356..5903
                     /gene="dnaX"
                     /locus_tag="VNN25_08795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXH91664.1"
                     /translation="MSYQALARKYRPQSFSDVIGQDTVVRTLQNAIEGNRIHHAYLFA
                     GVRGVGKTTAARILAKALNCIKGPTTEPCNECVICQEITEGIDMDVREIDAATYTGVD
                     NIRELRDTTQFQPARDRNRIFIIDEAHMLSTGAWNALLKILEEPPPHVVFMFATTEIN
                     KVPSTIISRVQKEMLRKITLDELIARIRRICASEEIEADKSAIEIIARRGEGSVRDSL
                     SLLDQVIAFSGRSISATDVATVLGLSDINFFARIAGYIADGDHGAILEALDEAAETGR
                     DFKMLYRDLLSFIRNLLLVAGNANESMLAVAPEDLPAIREAAKKFSYSELLRVANLLL
                     RDDETVNRAEHQRLAVEIALLKAATFPRLRAVEEALAGTPKAERRSDSPVRQTVKVGL
                     ESPTYVKPEPTAGDVDAFIERVQKARPLIGGYLAAAKSRRRDGNRLLLTFNDSFTAEQ
                     ITGARQALEQLAADVFGEPVSIDVQVDNPAAAAAPASKSSPLRDDPVVQAFQKHLGAD
                     LVESRKR"
     gene            5916..6215
                     /locus_tag="VNN25_08800"
     CDS             5916..6215
                     /locus_tag="VNN25_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXH91665.1"
                     /translation="MNPKQLMKQVQQMQEQMQQRMTALRVEGSAGGGMVKAAMNGSKE
                     LLAITIDKEAVDPNDVEMLQDLVVAAVNEASRKVDEEMASSVGAMTGGMKIPGLF"
     gene            6283..6876
                     /gene="recR"
                     /locus_tag="VNN25_08805"
     CDS             6283..6876
                     /gene="recR"
                     /locus_tag="VNN25_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXH91666.1"
                     /translation="MTAPPLANLINELSKLPGVGGKSASRIAYYILKRTTGEAESLAN
                     AIVDVKAKIFRCSTCNNMTDVEPCAICSDPRRDAGVICVVEEAFNIPTIESTRDFHGV
                     FHVLLGALSPLKGVGPQDIDVAGLLKRVQSGDVREVIVATNPNVEGEATALYIAQQLH
                     SLDVRTTRLAFGLPVGGDLEYADQVTMSKALEGRREI"
     gene            7033..7539
                     /locus_tag="VNN25_08810"
     CDS             7033..7539
                     /locus_tag="VNN25_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HXH91667.1"
                     /translation="MATPTPDLVMFEEEYQQIKDTLQRLQVDSNSKICFLVDKNGQQI
                     AVHGDMTGVDATSLASLTAGNVAATDGLAKLIGEKEFSILFHEGERDNIHISLVAQRV
                     ILVVIFDDRSSLGLVRLRVKKASAELERTFEVLLRKSEAERGNLANRFDSPFAEITDE
                     DIDSLFSE"
     gene            7546..8130
                     /locus_tag="VNN25_08815"
     CDS             7546..8130
                     /locus_tag="VNN25_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HXH91668.1"
                     /translation="MTFINYAAREINCKIVYYGPGLCGKTTNLQWIYDKTNPQAKGKL
                     ISLATETDRTLFFDFLPLDLGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEARMEANSESLVNLDKNLKEQGYELQSVPYVLQFNKRDLPTAIPADEMYRLLNF
                     KGEPTFEAVAMNGTGVFDTLKAVAKLVLTELKKK"
     gene            8166..9578
                     /locus_tag="VNN25_08820"
     CDS             8166..9578
                     /locus_tag="VNN25_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH91669.1"
                     /translation="MKVAFVKPPVGGILGLEMLTFVEPLGPICVAACLEAEGHECKVF
                     DLRIDGEEHGLDLCRKFAPDVVGLQCNFTTERNRTVRLAKRIKRENPGAFIVVGGHDA
                     SRDPSWFNDDVIDAVAVGDGEEVMPPMVDAIQRGHDLATIPGLMLHRDGRWVSTGHAP
                     TRKNLDDYPLPARHLIEQYADHYYINFHKPLALMETARGCPFKCNFCSVWKFHESTFR
                     EKSPARVVRELQAIKAEAIFITDDIFWMNVKRGEEMARQIKAAGIKKFFTVQTRTDII
                     CKFPHLIETWKDCGKLAIFLGLESVTDEGLKAVNKKNTADNNVKAIKILQDLGVGFTP
                     NFIVDPAWTRDDFKRLRDWIDHTGAYNSGFSVLTPLPGTDLWDTAKDQVTTHDWEMYD
                     IVHAVLPTTLSLDDFYEEYSGLWRHALDVRYRYRGKWKTYVQLGAAVATGKVSLHNVR
                     RGLGMAKTFSKPETFLRAHE"
     gene            9614..10660
                     /locus_tag="VNN25_08825"
     CDS             9614..10660
                     /locus_tag="VNN25_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HXH91670.1"
                     /translation="MRHHGLNIVAIGGGTGLSTLLRGLKRYVRSDGPWSIDHLGAIVT
                     VTDEGGSSGALRKEFGMLPPGDIRNCILALAEEEQLISRVFSYRFQNDESSLGGHSLG
                     NLLLTALTDITGGFDKAILTASEVLNIAGEIYPSTLSDVRLRATLADGSELIGEVAIS
                     GSYLGQKANQTPHARIVQLSIEPADAQPTARALEALAAADLILVGPGSLYTSILPNLL
                     IKPIGEALRQAKALRVYVCNVMTQPGESDNYSAEEHLAAIIEHAGLVVDAMILNGRRP
                     SESSLAAYAAKQQYPVAFNVDAIRELGVTPFFGDIIEEENYVRHNSAALCDTIFRLYD
                     RYGRTLERMRQTEA"
     gene            10657..>11214
                     /locus_tag="VNN25_08830"
     CDS             10657..>11214
                     /locus_tag="VNN25_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXH91671.1"
                     /translation="MTALKNTNLDVIILAAGLGTRMKSATIKILHRAAGRPIIEYVLD
                     LAAQVSDRSPIMVVGHQREAVQASVGDRARFAVQESQKGTGHAVLQAAEILEKENAAG
                     RKVLILSGDVPLTHPQTLQRLIDEHEQSQNALTLLTMKPADPGMYGRVVRDAGGAVIR
                     IVEAKDATDEEKQIGEVNAGIYVFDS"
BASE COUNT         2256 a         3492 c         3441 g         2025 t
ORIGIN      
        1 gaagagatcc gcatctcctc ggaagagatc cgcatctcct cggaagagat ccgcatctcc
       61 tcggaagaga tccgcatctc ctcggaagag atccgcatct gaaaaaagag atcgcgatct
      121 cccaaaacga gatccgcatc tcccaaagag agatcgcgat ctgtcaaaga cagatcatga
      181 cctgccggac agagatcgag accccgccga tcctgatcgc atccactcct ccgagatgag
      241 ccggcaagaa ggctcgatct cgactcgatg aaaggagaag gagctccctc ccggtaaccg
      301 ctccgacagg cccgtaacct ttccaatcgt gattcgtctc agagaataag ccggcggagg
      361 ttggaacctc gtggcacggc caaaggagaa agagatgtca acgaaaaaac gcaccggaaa
      421 ccgctcctcc caccccgtcg tccagaagcg ctccagaact aaaccaaagg ccatcgcggc
      481 catcccggac cttgtcgccg ccgcagcccg gatggtcgag ttgctcaacg aaatggaggc
      541 cgtcatcccc gatctgaccc agccggatat tgcgaagatc ccgcgcatgg ctcagagcgc
      601 gaagttcgga cgcgcgctca tcccgcccac gatcaacgcc gtcaattcct atccaccgtt
      661 tcagcagcgc aacatgttcg acgccgccgc cgcgcaggcg gccctcgact accagggcca
      721 gatgcagccg atcacgaaga ggatggccgc gattgtgatg gccatgtcgt attcgatgaa
      781 tcagaagatg tccgtctccg gcgaacaggc ccttcagttg tttcaatatg gcaagcgtca
      841 cgtgaagcag cctgacggcg gcggggcgcg gatctatgtc gatgaaatgc agagtgtcgt
      901 cgatcggacg atgaaccgcc gcaagccccg gaaacccgcc accaaccctc ccaccacacc
      961 accctcaaac actcctccca ccactcccac catgcagagc tttatggccc cttctcttgc
     1021 agcgaaggcg aggacgaagg cgcaaatcgt tccggatatc gatccggatg acgatgagat
     1081 ccgcgcgatc attcaaggaa tcgccacgga tccggagtag ccgcgcgtga caaggggtgc
     1141 cgcgtgaatg agaacgcggc gccctttcgc acgcaaacga cgactacggc cgtcgacgcg
     1201 cgcgatccga gaactgcggc cggtcgctgc tcgccgctcg gccattgcct cctgcatgcg
     1261 gacgcgcact cgatggacgc gcggcactcg ggcggccgga gcctggacgc gcggtcgggc
     1321 gcgagctggt gtacgtgttc gcacgctgcg gacgcggcgc gtgcgcgggg cgcgacggac
     1381 gccacgactt cagcggctcc gccgtcttat acgcgtttgc gtcgaacccc tcggcgacct
     1441 tgcgcggaat gccgcgggag atgaagcgct ccagatcgcg aaggttgccg cggtcgtcag
     1501 gcgtgacgaa gctgatggcg tcgccgatcg ctttggcgcg gccggtgcgg ccgatgcggt
     1561 gaacgtaatc ctcaggctgc ggcgggaaat cgaagttgac gacgtgggag atgccttcga
     1621 cgtcgatgcc gcgtgcggcg atgtcggtgg caacgaggac gcggaccgtg cccgccttga
     1681 atgccttgag cgccgcgatg cgctggctct ggctgcgatt cgaatgaagc gttccggtgt
     1741 tgatgccgga cgaagcgagc ttgcgggcga tcttgtcggc gccgtgtttc gtgcgcgaga
     1801 agacgagcac ggaatcgagt gagggatcgc gcagaaggtg gagaagaagc ggcagcttgg
     1861 cttccttcgt tgtctcgtag acgaactgcg tgacggtctc tgccggattg gcgcgcttgc
     1921 caacctggac gagctgcgag tcgctgagaa actcattcgt gagcgtctcg atctccttcg
     1981 acaacgtcgc cgagaacatc atcgtctgac gctgtttcgg aagcgctttc acgatgcggc
     2041 gcatggcggg gaggaagccc atgtccagca tgcgatcggc ctcatcgagc accgcgactt
     2101 cgagaccgga gaagtcgacg tgaccctgat ccatgaggtc gatgaggcgt cccggcgtgg
     2161 cgatgacaat ctcggtgccg gcacgcagcg cctggatctg cggccgctcg ccgacgccgc
     2221 cgtacaccgt ggcgatgcgg agatggagat gacgcgcgta cttgcggacg ttctcttcga
     2281 tctggacgac gagctcgcgc gtcggggcga tgacgatggc gcgcatgcag cggcgattcg
     2341 tcgccgagcc acgcgtcgaa aggcgctgca gaatcggcag cacgaacgcg gcggtcttgc
     2401 cggtgccggt ctgcgcgatg ccgatgacgt catggccgcg cagcacgagc ggtattgctt
     2461 tggtctggat gggggtaggt tctgtgtagc cggcttcacg cacggctttg gcgattttcg
     2521 ggtcgagccc gagtgcttcg aaaggcattt ggtgtgtccg ttcctgtcag agtatttgtt
     2581 cagctctgac gctcggacag aggtgaagca gtggtcgatc caacggatcg gaggcggcaa
     2641 aaattgctca gtgctgttcg accggcctcg ctagccagtc gtctttgccg gaggtgcacc
     2701 gtgctcggcg agaaattgcc tgagtgccgc gttcaccgac gtggcgtcgc ggaagacctc
     2761 ggctacgtcg ggagcaagga gcaccagatt cgtgtcttcc ctgtagcggg cgtagtactt
     2821 tccccttacg ccttccggag ccgcggtttg ctcggcgatt tcgagatcgc gatcgttatc
     2881 ctgattcata gagtcttcgt tctccggccg ttgcttcgcg tgccgtgatg attcgcgtcc
     2941 ggtcttcgcg ttcggtgtgc gccacgataa ccaatctgtg cgtattagtg tacccaagtg
     3001 tgagcattcg atattcctgc tcgtagtgtt cccgatcttg ccaggtcagt gcaaaatcat
     3061 ctccgaagac tgtggcggct tcctcgaaac taataccgtg cttcgatttg ttgcgccgat
     3121 ccttctcggg atcccattcg tacttcaccg actcgcagtc tacctcggcc ggctagcgag
     3181 tcacctcaac gccggaaggt aaacgcctta ctgatcgccg tcccctcgcc gtcgaagaat
     3241 tggatcgtca tcgaatggtc gtccagctcg aggacggcga atccgagctg cgttgtcgcg
     3301 tcggaaggga aaagcgtctt cgcgtgcggc acgatcggcg ggacgggatc ggatcctgct
     3361 ccgctgatca gataccgcgg ctggccgtcg atcagctcga ggtgatgatc gtgaccgcag
     3421 atgtagagat cgactcgggc ccgcttcatc accggctcga gcgtaaccat ccggacatgc
     3481 tgcgcgcgcg ggaagtggcc gtggtagccg gaggaaacga tcggatgatg gccgacggcg
     3541 atccgccacg acgtcttctt cgatgagaga gtctgccgca aggcatcggc ggccgcatcg
     3601 ctgcgggcga cagcgaaggg agtcgtgtcg agcatcagca gttcggcgat cctggcatcg
     3661 accacgtact gccttgccgg gaattgccag tgcgcgacag gagcagttcc gatctgcgcg
     3721 tccggcaccg acttgccgca gaagtcgtga ttgccgagga cgggaaagat cggaacgccg
     3781 atctggccga gcgcccggac acggctccat tgcggatcag tctttccagc cggaccgcag
     3841 ggatagaacg cgtcgccggt gatgatgacg gcgtcgagcg gtgtctttgc gtgaagtttc
     3901 gcgatccctc gggcaacgcg atccgatcct tcgccggtgt cgccgacgac ggcgatgcgc
     3961 agtgtcgtgc cccgaggctg gagcgcgacg tgagggagcg gcgcgccaag tgcaagcgct
     4021 gcgaacagca gatagacaat ggtgaacatg agttggtggc gatctacgca ggcaagaaca
     4081 tagccgatgc gtgctgcaca ttcgctggaa gttcttcgcg gccccctcgg tttcacgacc
     4141 tcgcgggtta caatccgcgt cccgtttcgg ctcgctggga tgagcctggg tcctgtgcag
     4201 caggcgcacc gaacctcgtc aggtccggaa ggaagcagcg ataaggtgtt cccctgtgtg
     4261 acgcagttcg gcctgggctc ttccgagcgg gccgcggcgg ctggttgcgc acggaactgg
     4321 ctttcgcgaa cgccgcaacc tagactctcg cccacatgtc ctaccaggcg cttgcgcgca
     4381 aataccggcc gcagtccttc agcgatgtca tcgggcagga cacggtcgtc cgcacgttgc
     4441 agaacgcgat cgaagggaat cggatccatc acgcgtatct cttcgccggc gttcgcggcg
     4501 tcggcaagac gaccgcggcg cgcatcctgg ccaaggcgct gaactgcatc aaaggcccga
     4561 cgaccgagcc gtgtaacgaa tgcgtgatct gccaggagat caccgaaggc atcgacatgg
     4621 atgtgcggga gatcgatgcc gcgacctaca ccggcgtcga caatatccgc gagctgcgcg
     4681 atacgacgca gtttcagccg gcccgcgacc gcaaccgcat cttcatcatc gatgaagcgc
     4741 acatgctctc gaccggcgca tggaacgcgc tgcttaagat cctcgaggag ccgccgcccc
     4801 acgtcgtgtt catgttcgcg acgacggaga tcaacaaggt tccgtcgacg atcatctcgc
     4861 gcgtccagaa ggagatgttg cggaagatca cgctcgacga gctgatcgcg cgcattcgca
     4921 ggatttgcgc gtccgaggag atcgaagcgg acaagagcgc aatcgagatc atcgcccggc
     4981 gcggcgaagg aagcgtgcgc gattcgcttt cgctgctcga tcaggtcatc gccttcagcg
     5041 gccgatcaat ctctgcaact gatgtggcca cggtcctcgg cttgtcggac atcaacttct
     5101 tcgcccgcat cgccggctac atcgcggacg gcgatcacgg cgcgatcctc gaggcgctcg
     5161 acgaagccgc cgagaccgga cgcgatttca agatgctgta ccgcgacctt ctgagtttca
     5221 tccgcaatct cctgctcgtc gccggaaacg cgaacgagtc gatgctggcc gtcgctcccg
     5281 aggatcttcc ggccattcgt gaggcagcaa agaagttctc gtactcggag ctccttcgcg
     5341 ttgccaacct gctgcttcgt gacgatgaga ccgtgaatcg tgccgaacac cagcgcttag
     5401 ccgtggaaat cgccctgctc aaggctgcga cgtttccgcg gcttcgggcg gtggaagagg
     5461 ctctggcggg tacgccaaaa gccgaacgta ggtcggactc tccagtccga cagaccgtga
     5521 aagtcggact agagagtccg acctacgtaa agccggaacc aacggcaggg gacgtcgacg
     5581 cgttcatcga acgcgtccag aaagcgcgtc cgctcatcgg cggctacctg gcagcggcaa
     5641 agtcgcggcg gcgcgacggg aacaggctgc tgctcacctt caatgactcg ttcaccgccg
     5701 agcagatcac cggcgcgcgc caggcgctcg aacagcttgc cgcagatgtg tttggggagc
     5761 cggtgtcgat cgatgtacag gtcgacaatc cggccgccgc cgcagctcct gcgtcaaaat
     5821 catcgccact ccgcgacgat cctgtcgtcc aggcatttca gaagcacctc ggcgcggatc
     5881 tcgtcgaatc acgaaagcgg tagggagaga gactcatgaa tccgaagcag ttgatgaaac
     5941 aagtgcagca gatgcaggaa cagatgcagc agcgcatgac cgcgctacgc gtcgaaggca
     6001 gcgccggcgg tggcatggtg aaggcggcga tgaatggctc gaaggagctg ctcgccatca
     6061 cgatcgacaa agaagccgtc gatccgaacg acgtcgagat gctgcaggac ctcgtcgtcg
     6121 ccgccgtgaa cgaagcctcg cgcaaggtcg acgaagagat ggccagctcg gtcggcgcca
     6181 tgactggcgg gatgaagatt ccgggactgt tttgagtcgg tcgcgcggtc tcggagttaa
     6241 gaaccacgtg actgcgcgac cccgtaaccg cgctaccgcg atatgaccgc tcctccactc
     6301 gcaaacctca tcaacgaact gagcaagctt cccggtgtcg gggggaagtc ggcgtcgcgt
     6361 atcgcgtact acatcctcaa gcggacgacg ggcgaggctg agtcgctggc gaatgcgatc
     6421 gtcgacgtga aggcgaagat cttccgctgc tcaacgtgca acaacatgac ggacgtcgaa
     6481 ccgtgcgcga tctgctccga tccgcggcgc gatgccggcg tgatctgcgt cgtcgaggag
     6541 gcgttcaata tcccgacgat cgaatcgacg cgtgactttc acggcgtgtt tcacgttctt
     6601 cttggcgcgc tctcgccgtt gaagggagtg ggcccgcagg acatcgacgt cgccgggctt
     6661 ctgaagcggg tacaaagcgg cgatgtgcgc gaagtgatcg tggcgacgaa tccgaatgtc
     6721 gagggggaag cgacggcgct ctatatcgcg cagcagctcc actcgctcga cgtaagaacg
     6781 acgcgcttgg ccttcggtct gcctgtcggc ggcgacctcg aatacgccga tcaggtaacg
     6841 atgtccaagg ctctggaagg gcggcgcgag atttgatttg tgagggtgtt gagggtcagg
     6901 agtcgggagt cggagccggg acgcttgcgc gtcggccttc gctacggccg ctcgatcccc
     6961 gactcccggc acccgcggtg tgctacattt ctccgtccag tccttcctga aactgtttgg
     7021 tgaaaatata agatggcgac cccgacccct gacctcgtaa tgttcgagga ggagtaccag
     7081 cagatcaagg acacgctcca gcgtctgcaa gtggactcca actcaaagat ctgcttcctg
     7141 gtcgacaaga acggccagca gattgccgtc cacggcgaca tgaccggtgt cgacgcgacc
     7201 tcgcttgcct cgctgacggc cggaaacgtc gcggcaaccg acggcctggc aaagctcatc
     7261 ggcgaaaagg agttctcgat cctcttccat gaaggggagc gcgacaacat acacatctct
     7321 ttggtcgccc agcgcgtaat tctcgtggtg atcttcgacg accgttcctc actcggcctg
     7381 gttcgtctgc gcgtgaagaa ggcctcggcg gagctggagc ggaccttcga ggtgcttctt
     7441 cgaaaatcgg aggccgagcg cggcaatctg gccaaccgct tcgattcgcc ttttgccgag
     7501 atcaccgacg aagacatcga cagcctcttc agcgaatagt ccggcatgac gttcatcaac
     7561 tacgcagcgc gcgaaatcaa ctgcaagatc gtctactacg gtcccggtct ctgcgggaag
     7621 acgacgaatc tgcagtggat ttacgacaaa accaatcccc aggccaaggg gaagctgatc
     7681 tcgctcgcaa cggagaccga ccggaccctc ttcttcgatt ttctgccgct cgacctcggc
     7741 accgtccgcg ggttcaagac gcgctttcat ctctacaccg tgcctggcca ggtcttctac
     7801 gatgcctccc gcaagctcat cctcaagggt gtcgacggcg ttgtcttcgt cgccgactcc
     7861 caggaggcgc gcatggaggc caacagtgag tcgctcgtga acctcgacaa gaacctgaaa
     7921 gagcaaggtt acgagctgca atccgtgccg tacgtgctcc aattcaacaa gcgcgacctg
     7981 ccaacggcga tcccagccga cgagatgtac cgcctgctca acttcaaggg cgagccgacg
     8041 ttcgaggcgg tggcgatgaa cggtaccggc gttttcgata cactaaaagc ggtcgcgaag
     8101 ttggtactca ctgagctgaa gaagaaataa gcacgctcct tctggcttta ccgggtgcac
     8161 acatcatgaa agtcgcattc gtcaaaccgc ctgtcggtgg gattctcggt ctggaaatgc
     8221 tgacctttgt cgagccgctc ggcccgatct gcgtcgctgc atgcctcgaa gcggaaggtc
     8281 acgagtgcaa ggtgttcgac ctgcgcatcg acggcgagga gcacggtctc gatctctgcc
     8341 ggaagttcgc gccagacgtc gtcgggctgc agtgcaactt caccaccgag cgcaaccgca
     8401 cggtccgtct cgccaaacgc atcaaacgcg agaaccccgg cgcgttcatc gtcgtcggcg
     8461 gacacgacgc ctcgcgcgat ccgagctggt tcaacgacga cgtgatcgac gcggtcgcag
     8521 tcggcgacgg cgaagaagtg atgcctccga tggtcgatgc cattcagcgc ggccacgacc
     8581 tggctacgat tcccggcctc atgctgcacc gcgatgggcg ctgggtcagc accggccacg
     8641 cgccgacccg caagaacctc gacgactacc cgcttcccgc gcggcacctc atcgagcaat
     8701 acgccgatca ctactacatc aactttcaca agccgctggc cctcatggaa acggcccgcg
     8761 gctgtccgtt caagtgcaac ttctgctcag tgtggaagtt tcacgagtcg acgttccgcg
     8821 agaaatcgcc ggcgcgcgtc gtccgcgagc ttcaagccat caaggccgag gcgatcttca
     8881 tcacagacga tattttctgg atgaacgtta agcgcggcga agagatggcg cggcagatca
     8941 aagccgccgg catcaagaag ttcttcacgg tccagacgcg caccgacatc atctgcaagt
     9001 tcccgcacct catcgaaacg tggaaggact gcggcaagct ggctatcttc ctcgggctcg
     9061 agtccgtgac cgacgaaggg ctcaaggcag tcaacaagaa gaacacggcc gacaacaacg
     9121 tcaaggcgat caagatcctc caggacctcg gcgtcggctt cacgccgaac ttcatcgtcg
     9181 accccgcctg gacgcgcgat gacttcaagc gcctgcgcga ctggatcgat cacacaggcg
     9241 catacaacag cggcttctcc gtcctcacgc cccttccagg caccgacctc tgggacaccg
     9301 caaaagacca ggtgacgacg cacgactggg agatgtacga catcgtccat gcagtgcttc
     9361 cgaccactct ctcgctcgac gatttttacg aagagtattc gggtctctgg cgccacgcgc
     9421 tcgacgtccg ctaccgctac cgcggcaagt ggaagacgta tgtgcaactg ggtgctgcgg
     9481 tggccacggg gaaagtgtcg cttcacaacg tgcggcgcgg actcggcatg gccaagacct
     9541 tcagcaagcc ggaaacgttc cttcgcgcgc acgaatgatt ccgcggaggc ggtgcgcgat
     9601 acgatagcgg gcgatgcgcc atcacgggct caacatcgtt gccatcggcg gaggaaccgg
     9661 cctctcgacg ctcctgcgcg gcctgaagcg ctacgtccgc tccgatggcc cgtggtcgat
     9721 cgaccacctc ggcgcaatcg tcaccgttac cgatgaaggc ggcagctccg gcgcgctgcg
     9781 caaagagttc ggcatgctgc caccgggcga cattcgaaac tgcatccttg ctctggccga
     9841 ggaggagcag ctcatctcgc gcgtcttcag ctatcgattt cagaacgacg agtcgtccct
     9901 cggcggacat tcactgggca atcttctgct cacggcgctg accgacatca ccggcggctt
     9961 cgataaggcc atcctcacgg cgtccgaggt gctgaacatc gccggcgaga tctacccgtc
    10021 aaccctttcg gatgttcgtc tccgcgccac gcttgccgac ggctccgagc tcatcggcga
    10081 agtagccatc agcggctcct accttgggca gaaggcaaat caaacgccgc acgcccgcat
    10141 cgtgcaactc agcatcgagc cggcggacgc gcagccgacc gctcgcgcgc tcgaggcgct
    10201 cgccgcagcc gatctgattc tggtcggtcc cgggtcgctt tacacatcca tcctcccgaa
    10261 tcttctgatc aagccgatcg gggaagccct gcgacaggcc aaggccctgc gcgtctacgt
    10321 ctgcaacgtc atgacgcagc cgggcgagag tgacaactac tcggcggagg agcatctggc
    10381 cgccatcatc gaacatgccg gactcgtcgt cgatgcgatg atcctcaacg gacggcgtcc
    10441 ctcggagtcg agcctggccg cctacgccgc aaagcagcag tacccggtcg cattcaacgt
    10501 cgacgcgatc cgcgagctcg gcgtgacgcc gtttttcggc gacatcatcg aagaggagaa
    10561 ctacgtgcgc cacaactcgg ccgcgctgtg cgacaccatt ttccggctct acgaccgcta
    10621 tggccggacg cttgaaagga tgagacagac ggaggcatga ccgctttgaa gaacacgaac
    10681 ctcgacgtca tcatcctcgc cgcgggcctc ggcacccgca tgaagtccgc aacgatcaag
    10741 atcctgcatc gcgccgcagg cagaccgatc atcgaatacg tactcgacct ggccgcgcaa
    10801 gtcagcgacc ggtcgccaat catggtcgtc ggccatcaac gcgaggccgt gcaggcatcc
    10861 gtcggcgacc gggcgcgctt cgctgttcag gagtcgcaga aaggaacggg ccacgccgtt
    10921 ctgcaggcag cggagatcct ggagaaggag aacgctgccg gccggaaggt tctcatcctc
    10981 tccggcgacg ttccactcac gcatccgcag acgcttcaac ggttgatcga tgagcacgag
    11041 cagtcccaaa acgcgctgac actcctgacg atgaaaccag cggatccggg catgtacggg
    11101 cgcgtcgtac gggatgctgg tggcgcggtc atccgaatcg tcgaagcgaa ggatgcgact
    11161 gacgaggaga agcaaatcgg cgaggtaaat gcgggtatct acgttttcga cagc
//
LOCUS       DATLVW010000285         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_55401, whole genome shotgun sequence.
ACCESSION   DATLVW010000285 DATLVW010000000
VERSION     DATLVW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55401"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VNN25_08835"
     CDS             <1..1425
                     /locus_tag="VNN25_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXH91672.1"
                     /translation="AKYGYDGLVVITIMAGVILVILGLARMGALIKFIPYPVITGFTS
                     GIAVIIFSSQLKDFLGLKMGAIPAEFMEKWTAYGEHLRSIDIPTTLIAVGTLVILVVW
                     PRVSRMVPAPFVAMIVATLVVQVFHLHVETIGSRFGAVASSIPAPHWPAIQWTHMKEF
                     VSPALTVALLAAIESLLSAVVADGMIGTRHKSNMELVAQGVANIASPLFGGIPATGAI
                     ARTATNIRAGGRTPLAGITHAVTLLLILVFLGRWAAMVPLCALAAILVVVAYHMSEWR
                     SFAGLLRAPRSDLVVLMLTFGLTIFVDLTVAVQVGIVVSALLFMKRMSDMTHVEGLTS
                     GFADDSDDPGEITESRRGRRIVAGHEIPLGVEVYEVNGPFFFGAADKIKDVLTEIAKP
                     PKVFILRMRNVPAIDATGIHALEQMAKKCRHEGTTLILTEIRQQPLRALVRARKLEAV
                     GGRENLAKSIEVALARAAEVLGKD"
     gene            complement(1644..>2280)
                     /locus_tag="VNN25_08840"
     CDS             complement(1644..>2280)
                     /locus_tag="VNN25_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXH91673.1"
                     /translation="INVGVATLLNYTAPVWSGLFSMFFIGEHFSARVLLPLPIALTGI
                     FLVVHAHAAPGDILGFGRWEIAGALSAVASGVAVTAIRAARRSENSWSVYASFCLIGT
                     LVCMPQGIAQWKTPHGAEWLSLGATALFAIGAQLLMTFSLRWVDAMTVGVISQLAVLV
                     STALGVLFLGDHIPLAAAIGGVLCISGVAGVTYVTSLSKPAVAAVEIVPEG"
BASE COUNT          414 a          676 c          760 g          430 t
ORIGIN      
        1 gcgaaatatg gttacgacgg gctggtcgtc atcaccatca tggccggcgt catcctcgtc
       61 atcctcggct tggcgcgcat gggcgcgctc atcaagttca ttccgtaccc ggtgatcacg
      121 gggttcacct cggggatcgc agtcatcatt ttctcgagtc agctcaagga ttttctcggg
      181 ttgaaaatgg gcgcgattcc ggccgagttc atggagaaat ggacggcgta cggtgagcac
      241 ctgcgctcga tcgacatccc gacgacgctg atcgcggtgg gaacactggt gattctcgtc
      301 gtatggccgc gcgtgtcgcg gatggtgccg gcgccgttcg tggcgatgat cgtcgccacg
      361 ttggtcgtgc aggtttttca tcttcacgtc gagacgatcg gatcgcgatt cggcgccgtg
      421 gcatcgtcga ttccggcgcc gcactggccg gcgatccagt ggacgcacat gaaggagttc
      481 gtctcgccgg cactgaccgt ggcgttgctg gctgccattg aatcgctgct ctccgcggtc
      541 gtcgccgacg gcatgatcgg cacgcgtcac aaatcgaaca tggagttggt ggcgcagggc
      601 gttgccaaca tcgcctcgcc gctcttcggc ggcatccccg cgaccggagc gatcgcgcgc
      661 acggcgacga acatccgcgc cggcggaagg acgccgctgg ccgggatcac gcacgccgtc
      721 acgctcctcc tcatcctcgt cttcctcggc cggtgggcgg cgatggttcc tctctgcgcg
      781 cttgcggcga tcctcgtcgt ggtcgcgtat cacatgagcg agtggcgctc gttcgccggc
      841 ctgttgcgcg cgccgcgcag cgatctggtc gtcctgatgc tgaccttcgg cctgacgatc
      901 ttcgttgatc tcacggtcgc cgtgcaggtg gggatcgtcg tttcggcgct gctcttcatg
      961 aagcggatga gcgatatgac ccacgtcgag ggcctgacca gcggttttgc cgacgacagt
     1021 gacgacccgg gcgagattac ggaatcaagg cggggcagga ggatcgtggc cggtcatgag
     1081 attcccctcg gagtggaggt gtacgaagtc aacggcccgt tcttcttcgg cgccgccgac
     1141 aaaatcaaag acgtgctgac ggagatcgcc aagccgccga aggtcttcat tcttcgcatg
     1201 cgcaatgtgc cggcgatcga cgcaactggc attcacgcgc tcgaacagat ggctaagaaa
     1261 tgccggcacg aagggacgac cctgatcctg accgagattc gccagcaacc gctacgggcg
     1321 ctcgttcgcg cgcgcaagct cgaagcggtc ggcgggcgag agaacctggc gaagtcgatc
     1381 gaagtggcgt tggcccgggc ggcggaagtg ctggggaagg actgaagaga tcagagagaa
     1441 gagagcagag aatcagagat cagagatcag agacaagacc agagacaagg accagagaca
     1501 agagatcagg ggcgccggtt tctcctgatc tcttgtctcc ggtgtcctga tgtctctgat
     1561 ctctgatctc tgatctctga tgtctttgat ctctagtctc tgatctctga tgtctctgat
     1621 ctctagtctc taatctctgt ccctcaccct tccggcacga tctccaccgc cgcaacggcg
     1681 ggcttcgata gcgacgtcac gtacgttacg cctgcgacgc cgctgatgca gaggacgcct
     1741 ccgatggcgg cggcgagtgg aatgtgatcg ccgaggaaga ggacgccgag ggcggtggag
     1801 acgaggacgg cgagctggga gatcacgccg accgtcatcg catcgaccca acggaggctg
     1861 aacgtcatca ggagttgagc tccgatcgcg aacagcgcgg tggcgccgag ggagagccac
     1921 tccgcgccgt gcggcgtttt ccattgggcg atgccttgcg gcatgcagac gagcgtcccg
     1981 atcaggcaga agctggcata gacggaccac gagttctcgc tgcgccgggc ggcgcggatg
     2041 gctgtgactg cgacgcccga tgcgaccgcg gagagtgcgc cggcgatctc ccatcgtccg
     2101 aagccgagga tgtcgcccgg cgccgcgtgg gcgtggacga cgaggaagat gccggtcagc
     2161 gcgatcggca acggaagcag cacgcgcgcg ctgaagtgct cgccgatgaa gaacatcgag
     2221 aataggccgc tccagacggg cgcggtgtag ttgagcaatg tggccacgcc gacgttgatg
//
LOCUS       DATLVW010000286        10828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_590147, whole genome shotgun sequence.
ACCESSION   DATLVW010000286 DATLVW010000000
VERSION     DATLVW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590147"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..602)
                     /locus_tag="VNN25_08845"
     CDS             complement(222..602)
                     /locus_tag="VNN25_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HXH91674.1"
                     /translation="MAHTSEHLRRFARGMRREPTRAESRIWGWLRARRFSEWKFRRQH
                     PIGRYIVDFYSAELKLAIELDGSQHAAEWMIDHESERTRELCSRGIEVLRIPNEMLIR
                     DSESVGESIKAAIERRVAVLHRLT"
     gene            complement(710..1570)
                     /locus_tag="VNN25_08850"
     CDS             complement(710..1570)
                     /locus_tag="VNN25_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXH91675.1"
                     /translation="MEPRRKGILFIVGAALLWSTGGIGIKAIADSALKVTFYRSLFAA
                     VALMLFLGRGVWARRQWKSTTAFIIAIISYGGCLTAFVIATKWTTAANAIFLQYAGVV
                     WVLLFSPLVVREPMRTRDAVAITVAFAGMALFFVGRFEAHGMAGNAMALLSSVFFASL
                     ILVLRREQRAAQAAVTWGNVVCALAVLPFVAHDLSLTPRSFAVLAFLGIFQIAIAYVL
                     FVRGLAFVTATQASLTGMLEPVSNPVWVFLFLGEKPSVFAIAGALVVLAAIAGHTLAG
                     EPAMDLPAPD"
     gene            complement(1742..2005)
                     /locus_tag="VNN25_08855"
     CDS             complement(1742..2005)
                     /locus_tag="VNN25_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91676.1"
                     /translation="MIDAIGWASSFILVLMIATQIHKQWKSGTSEGVSKWLFIGQLAA
                     STGFTIYSLLLRNWVFSVTNGVMLLSGVLGGAITVRQTRRARS"
     gene            complement(2013..2468)
                     /locus_tag="VNN25_08860"
     CDS             complement(2013..2468)
                     /locus_tag="VNN25_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91677.1"
                     /translation="MSATEARVLIIEDNDALRAMLFTVLRHQPLGVDTAIGAEDALEK
                     TRQCDYALILIDMNLPDDESISFLTRFREERPDGTSFILAVRDPNLEVAIDATHVSAI
                     VNKPLEIDTLAHAVRECALVVPPPDEPLNCPPAESDFRSRFDDSGAFFN"
     gene            complement(2517..2954)
                     /locus_tag="VNN25_08865"
     CDS             complement(2517..2954)
                     /locus_tag="VNN25_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91678.1"
                     /translation="MPTNERRILIVDDDDSIRGLLCTILSRRGFTVDTARNGIEALAR
                     LRTCNYAAMLLDLMMPMKSGYDVLDELKKSEAAQRPIVFVLTAGGAIRDLDPEVVAGS
                     IRKPFDVEVLTDMVTACVTACAPRPQLPDCPPAESAEARVKPS"
     gene            complement(3068..3658)
                     /locus_tag="VNN25_08870"
     CDS             complement(3068..3658)
                     /locus_tag="VNN25_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXH91679.1"
                     /translation="MTNLNGKRVAILVTDGFEQIELTSPREALDKAGATTVLVSPKDG
                     LVQGFKHHDKADKLSVDLPLAQANASDFDALVLPGGVINGDALRVEKRAQQFVQDMDR
                     AGKPIAVICHGGWLLISSGLVKGRRITTWPTLQDDMRNAGADWQDREVIRDRNLISSR
                     KPDDLPAFNKEMLHAITEAKPASARRAENREELGVR"
     gene            complement(4151..4456)
                     /locus_tag="VNN25_08875"
     CDS             complement(4151..4456)
                     /locus_tag="VNN25_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91680.1"
                     /translation="MIPVVADSRALPMIPPTGMAIAALTVMLWLIWSDTIRASRSPAV
                     LYAVRVALFVIVAGILVLNRVRYPFLFSATATVLVVVVALVGAGGAVYFARRLVKRA"
     gene            complement(4453..4740)
                     /locus_tag="VNN25_08880"
     CDS             complement(4453..4740)
                     /locus_tag="VNN25_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXH91681.1"
                     /translation="MDGNDEQPQHPAIRGLRTVCRCNNIKYKTIERAIRRGACTLTQI
                     AACTTATTGHCGGSCTPEVQEMLDEHAPQSKLSQPPPKPAASPDAWWIRKP"
     gene            complement(4727..4951)
                     /locus_tag="VNN25_08885"
     CDS             complement(4727..4951)
                     /locus_tag="VNN25_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91682.1"
                     /translation="MRSARIGKPRSASPGSFSLSRKLPRLSLPLSAAAATPARPRPSI
                     GKPGRSLFSGVPAGKPPLRDPVNPPRNGRK"
     gene            5277..6245
                     /locus_tag="VNN25_08890"
     CDS             5277..6245
                     /locus_tag="VNN25_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HXH91683.1"
                     /translation="MLILGLLVHLLLPRLDTITDSLKTLRSLAPWAIAIATLMEFLSY
                     VANGALLQSIVTLSGDRMTLRRAAVIEIGAATVALVAAGALGFGAAIYKWTRDSGVSG
                     NTAMLTSWLPSMFDAVSLILFALIGGVELLLFHRLSRITSSALIIVVSLLTFVIGGTI
                     VLLARHDWMMAVASRASRLVKRIRPKWDDAGLMRGAKGAAKTWRQLQHGGWIRPFVCS
                     LMNLIFDLLCLRYAFLAAGQPLRLSLLLAGYGVPLLLGRASFIPGGVAVVEVAMAALF
                     GGLGVPANAAVVAVLTYRLLSFWLPALIGIPIAATLQAHRREEKTA"
     gene            complement(6313..7137)
                     /locus_tag="VNN25_08895"
     CDS             complement(6313..7137)
                     /locus_tag="VNN25_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91684.1"
                     /translation="MNAGKVAAKWAGAGVLSVMLASTPAFAAGQWNGNGNRDGQNRDR
                     QSAQTQVQTDQRSADRTNYQHRNGQQQVNVAPVVTQPTYTAQRNDQYRSRNTNQSWSN
                     TNQSRDNAYQTRGRVDQASTYRQNTYRENERANISGRVQSFSRERNGYRVNLDRGSYW
                     IPESRFGNRARDLRVGVSLSLGGIFRGGVFGIDAVSWPASSGYGYDQGYVRGVVQQVD
                     YRSGTLILRDASTGRLFEVDMREAGGVQVGRGDYVTLSGQWIQGNVFAAYRVDSVG"
     gene            complement(7238..7690)
                     /locus_tag="VNN25_08900"
     CDS             complement(7238..7690)
                     /locus_tag="VNN25_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXH91685.1"
                     /translation="MRYWVNTVSRDHVQRGVDGGFTQAGHGKATGLKRMAKGDRIVFY
                     SPRTSLDGGEPLQAFTAIGTITDEAPFQVEMTPYFHPWRRKVVFATSQETPIRPLIES
                     LDFIRNKKSWGFVFRRGLFEIEEGDFRTIERAMTAFLVTGTSPPGSTS"
     gene            complement(7698..7883)
                     /locus_tag="VNN25_08905"
     CDS             complement(7698..7883)
                     /locus_tag="VNN25_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91686.1"
                     /translation="MNAKMKKLRAQIEKAGGRIMISDDIPEELAEMLTRVIGDCPDCA
                     AAMKASSRQQQHARHGH"
     gene            complement(8047..8508)
                     /locus_tag="VNN25_08910"
     CDS             complement(8047..8508)
                     /locus_tag="VNN25_08910"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXH91687.1"
                     /translation="MKNMKWFAAAAGAIALSATLAIAGTTTGDGGWKHGGKGGYERGA
                     HLAAKLNLTDTQKEQWKAVEQNFRQENAAFFEQSKQTREALHAAKKAGDTAQVDSLKA
                     TAKSQHAQMKQLRAAMEPKLMAILNADQQAQFQKLKAERAARGEGHEHEKQ"
     gene            8719..9882
                     /locus_tag="VNN25_08915"
     CDS             8719..9882
                     /locus_tag="VNN25_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXH91688.1"
                     /translation="MNATTQQPEHTRWSLKAREIFLIFLFWTSLAALSSVNRLLNRPD
                     QGFPVFSQAGPIAASFIESTIWAAMTIVIFWLSNRFSGQWRWITRITLLLVIGVVIAV
                     SVYLAVDLARDTLVTATRPRRVVNLSPWREVARFRFVNQFLIYLAVLAAGFAREYFLR
                     DQQRAREAIELARQTAALQAQLADARLDALRMQLNPHFLFNTLHAVSALVERDPGGVR
                     RMIARLSELLRHTMDSDAPNEVSLRDELSILRRYIEIMEIRFQGRLTVTAQIDDAALD
                     ALVPNLVLQPIVENALEHGAARASADGRIEITARLDDGERLVLTVRDNGPGVERNAGA
                     GVGLANTRARLEQLYGDEASLSLTSATGGGAISEIVLPFHTASDLRTEFRYDD"
     gene            9872..10684
                     /locus_tag="VNN25_08920"
     CDS             9872..10684
                     /locus_tag="VNN25_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXH91689.1"
                     /translation="MTTESEKPLRVLIADDELLARQRIEDLLAKEQGVEIVGTASTGT
                     ETVERIRELKPDLLFLDVQMPGLTGLQVAETIGAEEMPATIFVTAFDQFALKAFDVAA
                     IDYLVKPFDDERFVHALKRARKMIELEEVQKMTKRLLSLLHVPEGTAPPASEPPPKSK
                     YLERITVEMRGQLRVVPISKVDYVSASGPYAELHVGERTFAVRERMQTLEEQLDPNVF
                     FRIHRSAIVRLDRIDTLRRASGGDYAVRLKDGTELSLSRNRREELEKKLGAG"
BASE COUNT         1982 a         3405 c         3315 g         2126 t
ORIGIN      
        1 cccctcatcc ggcttcgcca ccttctcccc cccgcaaaaa gcgcgggggg agaaggcgct
       61 cgattcatga gcatcgcatg actacttgaa cgcgcaagca cgcgaacgcg tgactacgca
      121 gctatgtttt tcccttcacg actacctctc ccacgagcgc cttctccccc acgcttttca
      181 gtgggggaga aggtgccgaa ggcggatgag ggggtcgagc gtcatgtgag ccggtgcagg
      241 accgcgactc gccgttcgat cgcagccttg atcgattcac caacggactc cgaatcgcgg
      301 atcaacatct cgttcggaat tcgaaggact tcaatccctc ggctacacag ctcgcgggtt
      361 cgttcggact cgtgatcgat catccattca gcagcgtgtt ggcttccatc caactcgatc
      421 gcgagcttca attctgcgga gtagaaatcg acgatgtagc gcccgattgg atgctgacgg
      481 cgaaacttcc attcgctgaa tcggcgcgcg cgcagccatc cccagatccg cgattccgca
      541 cgagttggct cccgccgcat gccacgcgcg aagcggcgaa gatgttcgct cgtatgtgcc
      601 acgactccga ggatacctcg gtggattgcg ttgtgccttc ggaaaccagc aacaattctg
      661 atctcactaa accatcgcaa tgagctcgaa cgtctggcct gaaaccctgt cagtccggag
      721 ccggcagatc catggccggc tcccctgcca gagtgtggcc ggcgatcgcg gcgaggacga
      781 ctagggctcc ggcgatcgcg aagactgatg gcttctcgcc gaggaagagg aagacccata
      841 cgggattcga taccggttcg agcattccgg tgagtgaggc ctgcgtggcc gtgacgaatg
      901 ccaggccgcg gacgaagagc acgtaggcga tggcgatctg gaagatgccg aggaaggcca
      961 gcacggcgaa ggaacggggt gtgagtgaaa ggtcgtgcgc gacgaacggc aacacggcca
     1021 gcgcgcagac gacgttgccc cacgtcactg ctgcctgcgc ggcacgttgt tcgcggcgca
     1081 gcacgagaat caacgacgcg aagaagaccg aggagaggag ggccatggca ttgccggcca
     1141 tgccgtgcgc ttcgaaacgg ccgacgaaga agagagccat tcctgcaaag gcaacggtga
     1201 tggcgacggc atcgcgtgtt cgcatcggct cgcgaacgac cagcggcgaa aagagcagga
     1261 cccagaccac gcccgcgtac tgcagaaaaa tggcgtttgc cgccgtcgtc catttcgtgg
     1321 cgatgacgaa tgccgtgaga catccgccat agctgatgat ggcgatgatg aatgccgtcg
     1381 tcgatttcca ctgccgccgt gcccaaacgc cgcgcccaag gaagagcatc agggcgacgg
     1441 cggcgaagag ggagcggtag aaggtgactt tgagcgcgga gtcggcgatc gctttgatgc
     1501 cgatcccgcc cgtcgaccag agcagcgccg cgccgacgat gaacagaatc cctttgcggc
     1561 gcggttccat gaagggcggg attctatgcg ggagtcggag attcagagat cagagatcag
     1621 agacatcaga gactagagat cagggacgaa cctgatccct tgttttcctg atctcttgtc
     1681 tctgattgtc tctgatctct tgtctctgat ctcttgtctc tgatctctgt ctctacaccg
     1741 ctcacgaacg cgcgcgccgc gtttgcctga ccgtgatcgc ccctcccagc acgccactca
     1801 gcagcatcac accgttggtc acggaaaaga cccagttccg aagcagcagg ctatagatcg
     1861 tgaacccggt ggaggcggcg agctggccga tgaacagcca cttcgacact ccctcgctcg
     1921 tgcctgattt ccactgcttg tgaatctggg tggcgatcat cagcacgagg atgaaggagc
     1981 tcgcccagcc gatcgcgtcg atcacatccc actcaattaa agaatgctcc gctgtcgtcg
     2041 aagcgcgagc ggaagtcgct ttcggcggga gggcagttca aaggttcgtc gggcggtgga
     2101 acgacgagtg cgcactcgcg gaccgcatga gccagggtgt cgatctcgag aggcttgttc
     2161 acgatggcgc ttacgtgcgt ggcgtcgatc gccacttcca ggttcggatc gcgcacggcc
     2221 aggatgaagg atgtcccgtc gggccgttcc tcgcggaatc gggtcagaaa actgatgctc
     2281 tcatcgtcgg gaagattcat gtcgatgaga atcagggcat agtcgcactg ccgtgttttc
     2341 tccagcgcgt cttcggcacc gatcgcggta tcgacgccaa ggggctgatg acgaaggacg
     2401 gtgaaaagca tggcgcgtaa cgcgtcgttg tcttcgatga tgagaactct cgcttcggtg
     2461 gctgacatca gttctggccc tcttgccggt tgttgattct caaaagatgt gccagttcaa
     2521 ctcggtttca ctcgcgcctc ggcactctcc gcggggggac agtccggcag ttgaggccga
     2581 ggcgcgcagg cggtcacaca ggccgtgacc atgtcggtca ggacctccac atcaaacggt
     2641 ttccggatgg accctgccac gacctcggga tcgagatcgc ggatcgcgcc ccctgccgtg
     2701 agcacgaaca cgatcggtct ttgcgcggct tcgctcttct tcagttcgtc gagcacgtcg
     2761 tagccgctct tcatcggcat catcagatcg aggagcatcg ccgcgtagtt gcaggtgcgc
     2821 agacgcgcca acgcttcgat gccgttgcgc gccgtgtcca ccgtgaaacc gcggcggctc
     2881 aggatggtgc aaagcagacc gcgaatcgag tcatcatcat cgacaatcag gatccgcctt
     2941 tcgttcgtgg gcatgggtct ccagggaact ccacgaatca gaaagagcaa gtttgttgcc
     3001 ggtagccgaa aacaaatgcg ccaggcaaat gcctggcgca ttgaagcaat gaggtcactg
     3061 atgtctgcta gcgaacgccg agctcttcac ggttctcggc acggcgtgcg cttgcgggct
     3121 tcgcttccgt gatggcgtgg agcatctcct tgttgaacgc aggaaggtcg tcgggcttac
     3181 gactgctgat gagattgcgg tcgcggatga cttcgcggtc ctgccagtcc gcaccggcgt
     3241 tgcgcatgtc gtcctgaagc gtgggccatg tggtgatgcg gcggcccttg acgaggccgg
     3301 acgaaatgag cagccatccg ccgtgacaga tgacggcgat cggtttgccg gcccgatcca
     3361 tgtcctgcac gaactgctgt gcgcgtttct cgacgcgaag cgcatcaccg ttgatgactc
     3421 cgccgggaag aacgagggcg tcgaagtcgg acgcgttcgc ctgcgccagc ggaaggtcga
     3481 cgctgagttt gtccgctttg tcgtgatgct tgaagccctg gacaaggccg tctttcggcg
     3541 acacgagcac ggtcgtcgcg cccgccttgt ccaacgcctc tcgcgggctc gtcagctcga
     3601 tctgctcgaa gccgtccgtc acgaggattg cgacacgttt gccgttaagg ttggtcatgc
     3661 ttattacctc cggcatcggg gcatgcaaga tggggacact caacaatgga taagggagaa
     3721 ttgacaattg acaattgagc gtggtccctt tccgatctct ttgtcccttg tctctgattc
     3781 cttgtctcta atcactgccg aattgaggtt agtgtcatcc tgagactgcc ggagccgccc
     3841 gcgcgaatgg cgatgttcgc gcagtccgtg ctcggcggcg gaggacgtcg aaggatctca
     3901 aacttcgcag atcgtgttcg tccggcgcga gcgatcgtgt tcaaaccgct cgaacaagat
     3961 cttcgtattt tgagatcctt cgccgtcctg cggcgccgag gatgatctgc gcgagagcaa
     4021 cgtggtgcgc gggcgcctcc ggcaggctca ggatgacaca tcacgttttg cgtgtatcgt
     4081 tatggcttcg gcggttcagg atggcagcaa tcgcgtcatc caacggccat tctctctagc
     4141 ccgcgcgagg ttacgcgcgc ttcaccagcc gccgcgcgaa gtacacggca ccgcctgctc
     4201 cgaccagcgc gacgactacg acgagaaccg tcgccgtcgc cgagaagagg aacggatagc
     4261 ggacgcggtt gaggacgagg attccggcga cgatcacgaa gagcgccacg cgcaccgcgt
     4321 agagcacggc gggcgagcgg ctggcgcgaa tcgtgtcgct ccagatgagc cagagcatca
     4381 cggtcagcgc agcgatggcc atgccggttg gcggaatcat cggcagcgcg cggctgtctg
     4441 cgacgacagg aatcatggct tgcggatcca ccaggcatcg ggcgaagcgg caggtttcgg
     4501 aggcggctgg gacagcttcg actgcggcgc gtgctcgtcg agcatctcct gcacctcagg
     4561 cgtgcaactc ccgccgcagt gaccggttgt ggcggtggta catgccgcga tctgcgtgag
     4621 tgtgcacgcc cccctccgga tggcccgctc gatcgtcttg tatttgatgt tgttgcaacg
     4681 gcagacggtt ctcaggccgc ggatcgcggg atgctgtggc tgctcgtcat ttccgtccat
     4741 tgcgaggtgg gttaaccgga tcgcgtagcg gcggcttccc ggctggtact ccgctgaaga
     4801 gcgaccgccc cggcttgccg atcgacggcc gtggccgcgc cggtgtggcc gctgcagccg
     4861 acaacggcag cgataaccgc ggcagtttcc gcgagagcga gaacgaaccg ggagacgccg
     4921 atcgcggctt gccgatgcgg gccgagcgca tgaggccatt ttaccgcagg cgcgggggtc
     4981 gcgggtcggg agtcgggggt cggagcaggg gcagtagcgt gtgctggtgt tcgagagaag
     5041 agagaaagag atcaggagcg cgagcgatca gacgaaggat caggaacctg gggcgtacgc
     5101 tcccactccc cactccccac tccctacccc ccaactcctc ccgctccgac ccccgacaac
     5161 cgactcccgc acagacatag aattgaaaaa ggagctcaac cctgaccgac accgcatcca
     5221 cggaaagcga cgaatccagg ccgctgaagc tgcgcatcct gcgcgtcgct cccatcgtgc
     5281 tgatccttgg gctgctcgtt catctactcc tgccacgcct ggacaccatc accgattctc
     5341 tgaaaacgct ccgatcgctg gcgccgtggg cgattgcgat cgcgacgctg atggagtttc
     5401 tcagctacgt agccaacgga gcgctcctcc agtcgatcgt caccttgagc ggtgaccgca
     5461 tgacgcttcg ccgggccgcg gtgatcgaga tcggtgcggc aaccgtcgcg ctcgttgccg
     5521 ccggcgcgct cggattcggc gcggcgatct acaaatggac gcgcgacagc ggcgtttcgg
     5581 ggaacaccgc gatgctgacc agttggctgc catccatgtt cgatgcggtc agcttgattc
     5641 tcttcgccct catcggcggc gtcgagctgc ttctctttca caggctctcg cgcatcacga
     5701 gcagtgcgct catcatcgtt gtgtctctgc tgacgtttgt catcggcggc acgatcgttc
     5761 tgctggcgcg gcacgactgg atgatggcgg tggcctcgcg cgcctcccgg ctcgtgaagc
     5821 gcatccgtcc gaagtgggac gacgccggct tgatgcgggg agcgaagggc gccgccaaaa
     5881 catggcggca gttgcagcac ggtggatgga tcaggccgtt cgtttgttcg ttgatgaatc
     5941 tgatcttcga tctgctctgt ctccgctacg cattcctcgc cgccggccag cctcttcgcc
     6001 tctcgctcct tctggccggc tacggcgttc cgctcctcct cgggcgcgcc agtttcatcc
     6061 cgggaggcgt cgccgtggtc gaagtggcca tggccgcgct cttcggcgga ctcggcgtcc
     6121 ctgcgaatgc cgccgtcgtc gccgtcctca cctatcggct gctctcgttc tggctgccgg
     6181 cactgatcgg aatcccgatc gcggcgacgt tgcaggctca tcgtcgcgag gaaaagacgg
     6241 cttgagtgtg tgaatacgaa aaaagccggc cacgatgggc cggccttttt ccgttgcact
     6301 gcactcttcg tatcagccga cactgtcgac gcgataggca gcgaagacat tgccctgaat
     6361 ccactggccg gagagtgtga cgtagtcacc gcggccgact tggacaccgc cggcctcgcg
     6421 catgtctact tcgaacagcc ggccggttga cgcgtcgcga aggatcagcg ttccgctgcg
     6481 atagtcgacc tgctgcacca ctccacgaac ataaccctgg tcatatccgt agccgctgct
     6541 cgcgggccag ctcaccgcgt cgataccgaa gacgccgccg cggaagatgc cgccgaggct
     6601 gagcgaaaca ccgacgcgga ggtcgcgggc tcggtttcca aaccgcgact ccggaatcca
     6661 gtagctgcca cggtcgagat tgacgcgata gccattccgt tcccggctga acgactggac
     6721 acggccgctg atgttggcgc gctcattctc gcgataggtg ttctggcgat aggtgctcgc
     6781 ctgatcgacc cggccgcgcg tctggtacgc gttgtcccgc gactgattgg tattgctcca
     6841 cgactgattc gtgttgcgcg aacgatactg atcattacgc tgcgccgtat acgttggctg
     6901 cgtaaccacc ggcgcgacgt tcacttgctg ctgtccattc cggtgttgat agttcgtccg
     6961 gtcagcgctg cgctgatcgg tctgcacctg cgtttgtgcg ctctggcggt cgcggttttg
     7021 cccgtcgcgg ttcccgttgc cgttccactg cccagctgca aatgccggcg tcgatgcgag
     7081 catcactgac aacacgcctg ctcctgccca cttcgctgct acttttcctg cgttcatttt
     7141 gttcctcctc attacgcata aggagacaaa gcggcgagga gagaactttc gccaaaaacg
     7201 cgaacggcgc gcaggtgtca tgagctgcac cggcacgtca tgaagtgctg ccgggcgggc
     7261 tcgttccggt gacgagaaag gccgtcatgg cgcgctcgat cgtgcgaaag tcgccctcct
     7321 caatttcgaa caatccccgg cgaaagacga acccccagct cttcttgttg cggatgaagt
     7381 cgagcgactc gatgagagga cgaatcggcg tctcctggct cgtggcgaac acaaccttgc
     7441 gccgccaagg gtgaaagtag ggagtcatct cgacctggaa cggcgcctca tcggtaatgg
     7501 tcccgatggc cgtgaaggcc tgcagcggct cgccgccgtc gaggctggtt cggggcgaat
     7561 agaagacgat gcggtcgcct ttcgccatcc tcttcagccc ggtcgccttt ccgtgtccag
     7621 cttgcgtgaa gccgccgtcc acgccgcgct ggacgtgatc gcgagaaacg gtattgaccc
     7681 aatagcgcat ggccgcctca gtgcccgtgc cttgcgtgtt gctgttgccg cgatgacgcc
     7741 ttcatggccg ctgcgcaatc agggcaatcg ccaatcacgc gagtaagcat ttccgccaac
     7801 tcctcgggga tgtcgtccga gatcatgatc cgaccgcctg ccttttcgat ctgcgctctc
     7861 aacttcttca tcttcgcgtt catggaacac ctcctgacac cgaggttccc tcggaacgcg
     7921 acgaagcgca aattcttcta catggagtgg aattgttaag aagaaccaag caccacagag
     7981 acacaaagga cacagaggtt tctctgtgac ctctgtgtct ctgtggttaa tggctttcta
     8041 ctcgtactac tgcttctcgt gctcatggcc ttcgccacgt gcggcgcgct cggccttcag
     8101 cttctggaac tgcgcctgct gatcggcgtt gagaatcgcc atcagcttcg gctccatcgc
     8161 cgcgcggagc tgcttcatct gcgcgtgctg cgactttgcc gtcgccttca gcgaatcaac
     8221 ctgcgcggtg tctccggcct ttttcgcggc gtggagcgct tcccgcgtct gcttcgactg
     8281 ctcgaagaac gcggcgttct cctggcggaa gttctgctcg accgccttcc actgctcctt
     8341 ctgcgtgtcg gtgagattca gcttcgcggc gaggtgtgcg ccgcgctcgt acccaccttt
     8401 gcctccatgc ttccatccgc cgtctccggt cgtcgttccg gcgatggcga gggttgcgct
     8461 gagggcaatc gcacccgctg ctgctgcaaa ccatttcatg ttcttcatcg gtctttctcc
     8521 ttgggttgta tctggcagtc tgccagacgc cgatgaggac agttcggaaa cggctttcct
     8581 ttccgccggg aacgaagtgc acgcggatgt catgaagtgc atcgacgcga cacgaacaga
     8641 gacgacgagt cgctaagatg gctcaccagt ctgcagggcc agtcccttgc agcagatgga
     8701 ttgcttacgg atcgattgat gaacgccacg acacagcagc cggagcacac acgctggagc
     8761 ctcaaggcgc gcgagatctt cctgatcttc ctcttctgga cttcgctggc ggcgctgtca
     8821 tcggtgaacc gtctcctgaa ccggccggac cagggatttc cggttttctc gcaggcggga
     8881 ccgatcgccg cgagtttcat cgaatcgacg atctgggccg caatgacgat cgtgatcttc
     8941 tggctgagca atcgattcag cgggcagtgg cgctggatca cgcgcatcac tctgctgctc
     9001 gtgatcggcg tggtgatcgc tgtcagcgtc tatctcgccg tcgacctcgc gcgcgacacg
     9061 ctcgtcacgg caacccgccc ccgccgcgtc gttaatctct cgccctggcg cgaagtggcg
     9121 cgcttccgct ttgtcaacca gttcctcatc tatctcgcag tcctcgccgc cggcttcgcg
     9181 cgcgagtact tccttcggga ccagcaacgc gcccgcgagg ccatcgagct ggcgcggcag
     9241 accgcggcac tccaggcgca gctcgccgac gcccgcctcg acgcgctgcg catgcagctc
     9301 aatccgcatt tcctcttcaa cacgctccac gccgtttccg cgctggtcga gcgcgatccc
     9361 ggaggcgtgc gccgcatgat cgcccggctc agcgagctgc tccgtcacac gatggacagc
     9421 gacgccccga acgaggtctc gctgcgcgac gagctgtcga tcctgcgccg ttacatcgag
     9481 atcatggaga ttcgtttcca ggggcggctg acggtgacgg cgcagatcga cgatgccgcc
     9541 ctcgacgcgc tcgtccccaa tctcgtgctg caaccaatcg tcgagaacgc gctcgagcat
     9601 ggagcggccc gcgcatccgc cgacggccgc atcgagatca cggcgcgtct cgacgacgga
     9661 gagcgtctcg tgctgaccgt gcgcgacaac gggccgggcg tggagcgcaa cgccggcgcc
     9721 ggcgtgggcc tcgccaacac gcgcgcccgt ctggagcagc tttacggcga cgaggcttcg
     9781 ctttcactaa cgtccgcaac cggcggtggc gcgatctccg agatcgttct ccctttccac
     9841 acggcatccg acctgcgaac cgagtttcga tatgacgact gaaagcgaga agcccttgcg
     9901 tgtcctcatc gccgacgacg aactgctggc gcggcagcgc atcgaagacc tgcttgcgaa
     9961 ggagcagggc gtcgagatcg tcgggacggc gtcgaccggg acggagacgg tagagaggat
    10021 ccgggagctg aagccggatc tgctctttct cgacgtccag atgccggggc tgaccggcct
    10081 gcaggtcgcg gagaccatcg gcgccgagga gatgccggcc acgatcttcg tcacggcctt
    10141 cgatcagttc gcgctgaaag cattcgatgt cgccgcaatc gactacctcg taaaaccatt
    10201 cgacgacgaa cgcttcgtgc acgcgctgaa acgagcccga aagatgatcg agctcgaaga
    10261 agtgcagaag atgacgaagc gcctgttgtc gctgctccat gtccccgagg gcacggcacc
    10321 tccagcctcc gaaccgccgc ccaaatcaaa gtacctcgag cgcatcaccg tcgaaatgcg
    10381 aggccagctc cgcgtcgtcc ccatctccaa agtcgactac gtcagcgcca gcggccccta
    10441 cgctgagttg cacgtcggcg agcgcacctt tgccgttcga gagcgcatgc aaaccctcga
    10501 agagcagctc gatcccaacg tcttcttccg catccaccga tcagccatcg ttcgcctcga
    10561 ccgcatcgac acgctccgcc gcgcaagcgg cggcgactac gccgtccgcc tgaaagacgg
    10621 cacggagctg agcctcagcc gcaaccgaag agaggagttg gagaagaagc tgggggcggg
    10681 gtagccgaag tctcacgcga agacgcgaag gcgcgaaggg ttttgacgtt gcgatgctct
    10741 ccttcgcggt cttcgcgtct tcgcgtgaga caagttgcgg caagggttcg aaaaaccctc
    10801 tttgtgacgc agctcacgaa acctccgt
//
LOCUS       DATLVW010000287         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_590166, whole genome shotgun sequence.
ACCESSION   DATLVW010000287 DATLVW010000000
VERSION     DATLVW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590166"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1097)
                     /locus_tag="VNN25_08925"
     CDS             complement(318..1097)
                     /locus_tag="VNN25_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91690.1"
                     /translation="MRHRILILALSLLATQAFAARTSGFALNVLVDDVARSEYFHGGT
                     VYIEAVRGSSYSLRLTNPTPYRVAVALSVDGLNTIDAKHTDARSASKWVLEPYESTTI
                     SGWQVSDRAARSFYFTGERHSYGARLGQTENLGLIEAVYFRERQREPEVYRHSFGEGR
                     SNAAESGSAQPSPSAGLAAPSAKAQRHDTSDDYAATGMGDKAEHEIYSVDMDLDPTPI
                     ASVRIRYEFRQQLVKLGILPQHISPLERREHASGFGAYCPE"
     gene            1276..>3522
                     /locus_tag="VNN25_08930"
     CDS             1276..>3522
                     /locus_tag="VNN25_08930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXH91691.1"
                     /translation="MRKQWISCLFLVAFAAASFAAPATAKKATTAAKTAAVKITSVEG
                     ITEYDLANGMRVLLFPDQTKPTVVINLTYLVGSRHESYGETGMAHLLEHMMFKGTPDH
                     KNIPQALKEHGANYNASTWYDRTNYFEILPATEDNVKFALALEADRMQHSFIARKDLD
                     SEMTVVRNEFESGENDPENVLDERVLSTAYLWHNYGHSTIGSRADIERVPIDRLQAFW
                     RKYYQPDNVVLLVAGKFDEAKTLAEVEKLYGSIPRPTRKLEEPYTAEPAQDGERAVTL
                     RRVGDVQALTVAYHVPAEAHPDSAAAQVLLRLLIDTPSGRLYKALVEPKKATSVGGFM
                     PGLHDPGFVTLSAEVRQEQSLEDAKKTFLQTIDDLKTTPPTKEEVDRARTGMLKNIDM
                     TLNAADRVGIALSETIAAGDWRLFFLNRDRIRKVTPADVQRIANTYFIASNRTVGEFI
                     PDKTPVRAEIPPPSDVAAIVKDYKGDAAIAAGEAFDPSPANIDSRTTRTTLPGGLKIA
                     LLPKKTRGGTVNVSGNLHFGDEKSLMNRSTAGVAAADMLMRGTSKHTRQQLKDEFDKL
                     KARVNIGGGATQLSVNVETTRENLPAVLALVGEILHDPVFPASEFSQWKQEQLAQAEQ
                     QRSDPQSVGSTEFQRRMNPYPKGDVRYVTTSEEDIADINALTLEDVKNFYSTFYGASN
                     AEIALVGDFDPKQVTAQLTEIAGSWKSTTPFTRVPRIYHDVAPTNQSLETPDKANAIF
                     LAGVNLQLR"
BASE COUNT          720 a         1064 c         1123 g          615 t
ORIGIN      
        1 cctcgtgtcg caccgcgacc gttccatgga agtctggacg cgcgaaaccg acaaccgctg
       61 gcgctcagtc atcgtgcgag aaggcgcggc cgcggagttg ctggtcggcg cgcacctcga
      121 tgttcacgag ctctacgagg ctgcggccga gccgagagag tgaatcgagt gtagtgacga
      181 gatgaatgaa ccacagagac acagaggtca cagagattaa gtgctgcctc tgtgacctct
      241 gtgtctctgt ggttgagcga cgcgcggctt aaggatgctg cacgtccgaa gccgcgcgcg
      301 ctgtcaaaaa cgcgagctta ctccgggcag tacgccccaa acccgctcgc gtgctcccgt
      361 cgttccagcg gcgagatgtg ttgggggaga atgccgagct tgacgagttg ctggcggaac
      421 tcgtagcgga tgcggaccga ggcgattgga gttgggtcga ggtccatgtc gacgctgtag
      481 atttcgtgct cggccttgtc acccatgccg gttgcggcgt aatcgtcgga cgtgtcgtgt
      541 ctttgcgctt tcgcggaagg tgcggccaag cccgcggacg gagacggttg agcactgccg
      601 ctttcggcgg cgttggatcg tccctcacca aacgaatggc ggtagacctc gggctcgcgc
      661 tggcgttcgc ggaaatagac ggcttcgatg aggccgaggt tttcggtctg gccgagtctg
      721 gcgccgtagg agtggcgctc gccggtgaag tagaagctgc gcgcggcgcg gtcgctgacc
      781 tgccagccgg agatcgtcgt cgactcgtac ggctccagca cccatttcga ggcgctgcgg
      841 gcgtcggtgt gtttggcgtc gatcgtgttg aggccgtcaa ccgacagcgc gacggcgacg
      901 cgatacggcg tcgggttcgt caggcgcagg ctgtagctcg aaccgcgaac ggcttcgatg
      961 tagacggtac cgccgtggaa gtactccgat cgagccacat catcgacgag cacgttgagt
     1021 gcgaatccgc tcgtgcgggc ggcgaaagcc tgagttgcca gaagtgagag agcgagaatt
     1081 agtattcggt gtcgcatgga atcctccgtt atgggatgtg agacacatgc gttgggaaaa
     1141 agaaaaccgg tcacgcggag acgcggggga cgcagagggt tctattcgcg tcttcgcgtc
     1201 ttcgcgtgaa agtggcgcat gcgcagcgga agttgcagaa tggcgtccct aacccacgaa
     1261 accagaggag accccgtgag aaaacagtgg atatcgtgcc tgttcctcgt cgcgtttgcg
     1321 gcggcgtcgt tcgccgcgcc ggcgactgca aagaaagcaa cgacggcagc gaaaaccgcg
     1381 gccgtgaaga tcacgtcggt cgaaggaatc accgagtacg accttgccaa cggcatgcgc
     1441 gttctcctct tccccgatca gaccaaaccg acggtcgtca tcaacctcac ctacctcgtc
     1501 ggctcgcgcc acgagagcta cggcgagacc gggatggccc atctcctcga gcacatgatg
     1561 ttcaagggga cgccggacca caaaaacatc ccgcaggcgc tcaaagagca cggcgccaac
     1621 tacaacgcct cgacctggta cgaccggacg aactatttcg agatcctgcc ggccaccgag
     1681 gacaacgtga agttcgctct tgcgctcgaa gccgaccgga tgcagcactc cttcatcgct
     1741 cgcaaggacc tcgactccga gatgaccgtc gtgcggaacg agttcgagtc gggcgaaaac
     1801 gatccggaga acgttctcga cgagcgcgtt ctctccaccg cctacctctg gcacaactac
     1861 ggacattcga cgatcggttc gcgcgcagac atcgagcgcg ttcccatcga tcgcctgcag
     1921 gctttctggc gcaagtacta ccagcccgac aacgtcgtcc tgctggtggc cggaaagttc
     1981 gacgaagcga aaacgctcgc cgaggtcgag aagctttatg gatcgattcc gcgtccgacg
     2041 cgcaagctcg aggagccata cacggccgag ccggcacagg atggggagcg tgccgtcacg
     2101 ctgcggcggg ttggcgacgt tcaggctctg accgtcgcct atcacgtgcc ggcggaggcg
     2161 catcccgatt cagcggcagc gcaggtgttg ctgcgccttc tgatcgacac cccctcgggc
     2221 cgcctttaca aagcgctcgt cgagccgaag aaggcgacgt cggtcggcgg tttcatgcct
     2281 ggcctgcacg atccgggatt cgtgaccctg tcagccgagg tgcggcagga gcagtcgctg
     2341 gaggatgcga agaaaacgtt cctgcagacc atcgacgact tgaaaaccac gccgccgacg
     2401 aaagaggaag tcgatcgcgc gcgcaccggc atgctgaaga acatcgacat gacgctcaac
     2461 gccgcggacc gggtcgggat tgctctcagc gagacaatcg ccgccggaga ctggcgcctc
     2521 ttcttcctca accgcgaccg catccgcaag gtcacgccgg cagacgtgca gcgcatcgcg
     2581 aatacgtact tcatcgcttc gaaccggact gtcggcgagt tcatccccga caagacgccg
     2641 gttcgtgctg agattccgcc gccgtcagat gtcgctgcga tcgtcaagga ctacaaaggc
     2701 gacgctgcca ttgcagccgg cgaggcgttt gatccctcgc ctgcgaacat cgattcacgc
     2761 acgacgcgca cgacattgcc gggcggcttg aagattgccc ttctgccgaa gaagacacgc
     2821 ggcggaacgg tgaatgtctc cggcaacctg cacttcggcg acgagaagtc gctcatgaat
     2881 cgttcgacag cgggcgtggc cgcagccgac atgctcatgc gcggaacgag caagcacacg
     2941 cgccagcagc tcaaggatga attcgacaag ctcaaagcac gcgtgaacat cggcggcgga
     3001 gcgacccagc tcagcgtcaa cgtggagaca acgcgcgaga atcttccggc ggtgctggcg
     3061 ctggtcggcg agatcctgca cgatcccgtt ttccctgctt cggagttcag tcagtggaag
     3121 caggagcagt tagcgcaggc ggagcagcag cgcagcgatc cgcagtcggt cggctcgacg
     3181 gagtttcagc ggcggatgaa cccttatccg aagggtgacg ttcgctatgt aacgacgtcc
     3241 gaggaagaca tcgcggacat caacgcgctc acgctcgagg acgtgaagaa tttctactcg
     3301 acgttctatg gcgcttcgaa tgccgagatc gcgctcgtag gcgacttcga tccgaagcag
     3361 gtaacggcgc agctgactga gatcgcaggc tcgtggaaga gcacgacgcc attcacccgg
     3421 gtgcctcgga tctatcacga cgttgcgccg acgaatcagt cgctggaaac gccggacaag
     3481 gccaacgcca tcttcctggc cggagtcaat ctccagcttc gc
//
LOCUS       DATLVW010000288         6521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_179616, whole genome shotgun sequence.
ACCESSION   DATLVW010000288 DATLVW010000000
VERSION     DATLVW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179616"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..961
                     /locus_tag="VNN25_08935"
     CDS             110..961
                     /locus_tag="VNN25_08935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXH91692.1"
                     /translation="MTTEPLIRNISDTALWVAMYRAWETEQPNPVFRDPYARRLAGVR
                     GEEIMSKIKVAHRHAWSYTARTWNIDSFVTQEIANGADMVIDLAAGLDTRPYRMELPS
                     SLQWIEIDLPELLTYKDQVLKGEKPVCRLERIPFDLANAGARRVLFEELGRAAKRAVI
                     ISEGLIVYLTADEVSTLARDLAAPASFQRWATDLCSPGLLKMLQKQVGGQLGQAGAPL
                     KFAPEQGPAFFTEYGWTPIDVRSMLQTAATLKRLPLLMRLFALFPDGKGTKPKQIWGG
                     VVQLEKK"
     gene            1695..2309
                     /locus_tag="VNN25_08940"
     CDS             1695..2309
                     /locus_tag="VNN25_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91693.1"
                     /translation="MSIPPAPASPPLGPPPVTGRERALRIIVGLASLGLALFVALSPN
                     VVYAPAPKAILYLLVALLPAILFAAELTAQFQLQLGWFVASAGGAAALVLITLVLLTH
                     LTKPEEQIAVFEIVDDAEQPVSGLDRTGAVQVPRTANGLTITPLVDGNSIVLIFPEQA
                     PVVDLLVRPVLNGPVYRGRVSYAGTRKMKLVLGRDLVAKGGSKS"
     gene            2309..3238
                     /locus_tag="VNN25_08945"
     CDS             2309..3238
                     /locus_tag="VNN25_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91694.1"
                     /translation="MWRAFFVSLGIIVLPLIAAVPTVPDPVANLQRKYSWGSIFLTYS
                     SLADLANTFQNKALASGLATHGDADALGLLKRFVTQHTSVRYGESAAFLQLDLAAQRA
                     GFMPTNAVDGTVTPLPWSPAEVGRVALLIDKYLSANSSAIAFTRELAKRTDVAGSWQQ
                     LTALANNPLVAPRITYSAKIAFRQLMEVRAGELKKLRTTDPTLMQALDDFRHHVDEEI
                     AMRPFDNQAMPGPGQAWANYWEYAQYPTGTTSTTGSDPGWYGSLSTSATDTTGWYSTS
                     MTSTSTTTESTDPMSSTSTTGTNTTTETTSTRL"
     gene            3457..4458
                     /locus_tag="VNN25_08950"
     CDS             3457..4458
                     /locus_tag="VNN25_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HXH91695.1"
                     /translation="MADDDLLLVPPVPDALREAAQVAGNLIPFIGAGASRLAGCPSWI
                     ELADAAFNCFVDQGKFSHAQLDQIKSLNPRVKLSLAVGMQDELRITIDFKKLIHPVER
                     QDHANGRRLYGILAKLGRTFVTTNCDEWLDEVLPEPSLSVHGVADGRVGAVPKRRDVF
                     VDREELTAANLNREGAVLHLHGSVRKPEGMIITTRHYVKHYENDRRLKTTDAENPVLT
                     FLEFLFRTKTVLFIGYGLEELEILEYVILKTRDLKTPNAQPRHFILQGYFSHQRELML
                     SMRRYYRDCGIELLPFLMDPRGHEQLIEVLDAFARTVPPSTLAVLQEFEEMETLLNG"
     gene            4451..>6521
                     /locus_tag="VNN25_08955"
     CDS             4451..>6521
                     /locus_tag="VNN25_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91696.1"
                     /translation="MANLSANEKSFVTLMRKSDEHARRGFDLLAKRQDPEKFFDELAA
                     QGAFSPSEAPAPVRVDENSIRFPQWPVLNYLLQCARISDEQNDIALAQSVMGVVRAVM
                     AFREEDGNIRDNYVTFWRFAEILGAVPNAVVTIDDIELASIWLNSRFDRMMVASALSE
                     GIFRRCLASNDPADWNKAIQLLPHCLAIVWQSERGGIDKEEPVTIVDPYWLKKLIRQY
                     AEEFGKKVGSAAATIFRERVQELFSKGGRVSWSYVFRPAVEDHAQNHSWHDADNCLVE
                     GLRDVLLAWSDIELAGAKLFVDDLLRSNVEMLRRIGIYVLDERWNQLGGLYDGIVGTD
                     LFASGHIHELFHLLRNHFEQFDEEQKMATVAAIREIGPPEVEDAELVLKWIQRRWLEA
                     IAGTTYEPVVTWIAELARDPRVSVQIHPDFSTYMEAAWGPGPSPYQVEELVAFVRHGT
                     LVEKLNAFMQRPEWRGVSSEALVEALRQAVVVAPAAFARALPPFLAAKPTYQHGVIGG
                     FKRLWDAPKEQQASADWEEIWLNLFDFLEKLFEDPQAGRRSEVDAGFAQSWLVSETAD
                     LLDAGTRDDKHAYPTALLPRGWALLSILAERAEAAEQPDDRDPMTQALNTPKGRTIGA
                     IVGHALRESRLADKNGGSHAAVWNGLRPLFDQELEKCETGNYEFATLAGASIANLEYL
                     DADWLRAH"
BASE COUNT         1413 a         1854 c         1947 g         1307 t
ORIGIN      
        1 gcctggttgt cgcaccaact ttcacgcgaa gacgcgaagg tgcgaacaaa ccctcttcgc
       61 gccttcgcgt cttcgcgtga ctacccaaca tcgcatacaa cggagaacca tgaccaccga
      121 gcctctgatc cgcaacattt ccgacacggc actctgggtg gcgatgtacc gcgcctggga
      181 aacggagcaa ccgaatcccg tctttcgcga tccttacgcg cgccgcctgg ctggtgtgcg
      241 cggcgaagag atcatgtcga agatcaaagt ggcgcatcgt cacgcttggt cgtacaccgc
      301 tcgcacctgg aacatcgatt ccttcgtgac gcaggagatc gccaacggcg ccgacatggt
      361 gatcgatctc gctgccggcc tcgatacacg gccgtatcgg atggaattgc cgtcgtcgct
      421 gcagtggatc gagatcgatc tgccggagtt gctgacgtac aaggatcagg tattgaaggg
      481 cgagaagccc gtttgccggc tggagcgcat tcccttcgac ctagcgaacg ccggcgcccg
      541 ccgcgtgctg ttcgaggagc tcgggcgcgc cgcgaaacgg gccgtcatca tcagcgaagg
      601 cttgatcgtc tatctcaccg ccgatgaagt cagcaccctg gcgcgcgatc tggcggcccc
      661 ggcttcgttt cagcgctggg cgacagacct ctgttcgcca ggattgctga agatgctgca
      721 gaagcaggtc ggagggcagt taggtcaagc cggagccccg ctcaaattcg ccccggaaca
      781 aggtccagcc ttcttcaccg agtacggctg gaccccgatc gacgtgcgtt cgatgcttca
      841 aaccgccgca acgctgaaac gcctcccgct gctcatgcgc ctcttcgctc tatttcccga
      901 cggcaaagga acaaagccga agcagatctg gggtggggtc gtgcagttgg aaaagaaatg
      961 acgccggtgg tcattcaaag gtcgccgctg gtcatgaata tctgagcgtc agacattcgc
     1021 gatgtcggtg ggacccgcga atgtctgacg gtcagacatt cgcgatgacg cggatcatcc
     1081 cgcgagacga tttcgcagaa tcgaatatct gacgctcaga cgttcgctat gacggcggac
     1141 gtcattcata gctcgccgct ggacatgaat atctgagcgt cagacattcg caatgtcgac
     1201 aggacccgcg aatgtctgac ggccagacgt tcgcgatgac gcggatcacc ccgcgagaca
     1261 atttcgtaga atcgaatatc tgacgctcag acgttcgcat caacagtgga agtcattcaa
     1321 aagccgccgc gagtcatgaa cgtctgagcg cccgacactc gcatagtcgc cggagccgcg
     1381 aacgtccggc acgcaggtgc tcgcgatgcc gcggcagccg ttcgaagctg gcttcgagcc
     1441 gcgaataacc gccgcagcgc taaaacgaat cccgccgctg atgcgcctct tcgcccaccc
     1501 caaaggaacg aagccgacgc agatctgggg cggggtcgtg cagttggaga agaaatggcc
     1561 ggtcgacgtc aacatagacg accgtacgtc gttaccctat cactgggatt cgaccggatg
     1621 ccgtggccaa attgcgtcgc tggcttcttt tcacgtgcac gcagtgaggc acccgtatat
     1681 aattagatat ccacatgagt attccgccgg caccggcgtc accaccgctt ggaccacctc
     1741 cggtcacggg gcgtgagcgc gccctgagaa tcatcgtcgg acttgcaagt ctgggactag
     1801 cattgttcgt cgcgctctcg ccaaacgtcg tgtacgcgcc agcaccaaag gcgatcctct
     1861 acctactcgt agcgctcctc cccgcgattc ttttcgcagc ggagcttacc gcacagttcc
     1921 aactccagct ggggtggttt gtcgcaagtg ccggtggcgc cgctgcactc gtgctcatca
     1981 cgcttgtcct cttgacacat ctcacgaagc ccgaagagca aatcgcggtt ttcgagatcg
     2041 tcgacgatgc ggaacagcca gtaagcggac tcgatcgcac tggcgcagtc caagtgccgc
     2101 gaaccgctaa cggcctcacg atcactccac tcgtggacgg taactcgatc gtcctgatct
     2161 ttcctgagca agcgccggtc gtcgatcttc tcgtgcgccc agtcctgaat ggtccggtgt
     2221 accggggacg cgtctcttat gcgggcacgc gaaaaatgaa gctcgtgctg ggacgggacc
     2281 tcgttgccaa ggggggtagc aaatcatgat gtggcgcgcg ttctttgtca gccttggcat
     2341 catcgtcctt cctctcatcg cagcggtacc gacggtgcca gacccagtgg cgaatctgca
     2401 acggaaatac tcttggggca gtatttttct gacatactcc tcgctcgcgg atctcgcaaa
     2461 cacgttccag aacaaggcgc ttgcgtcagg cctcgccaca cacggcgacg cggatgcact
     2521 gggtctgctg aagcggttcg tcacgcagca caccagcgtg cggtacggcg agagcgctgc
     2581 cttcctccaa ctagatctcg ctgcgcagcg ggccgggttc atgccgacca acgcagttga
     2641 cgggacggtc acgccgctcc cttggtcgcc agcagaagtt ggcagagtag cgctgctcat
     2701 cgacaagtat ttatcggcga attcgtcggc gattgcgttc acgcgtgagc tggcgaaacg
     2761 gacagacgtc gcgggttctt ggcagcagtt aacggcgctc gcaaacaacc cgctcgtcgc
     2821 gccacgcatc acatacagcg caaagattgc ctttcgtcag ctcatggaag tgcgagccgg
     2881 agagctgaaa aagctgcgca ccaccgatcc cacactaatg caggcgctcg acgacttccg
     2941 gcaccacgtc gatgaggaaa tcgctatgag accgtttgac aatcaggcaa tgccgggtcc
     3001 agggcaggcc tgggcgaact attgggagta cgcgcagtac ccaacgggga cgacatcaac
     3061 gaccggaagt gacccgggct ggtacggctc gctgtcgaca tcggcaacgg acaccaccgg
     3121 gtggtacagc acgagcatga ctagcacgtc cactacgacc gagtcaactg acccaatgtc
     3181 cagtaccagc acgaccggaa caaatacgac aactgagaca accagcacgc gtctgtaatg
     3241 gacgggtacc agtaagtact gtggacttca aagcgtcacc ggagctgatg ccccgaggcc
     3301 cgaaccgtaa gatgttgggt cgtatctgaa gacttaacgt aacgcagcac ggtcttggat
     3361 gacctcctcc gataagctga ttcttccgat acggccccgc aaatgccaca gaaaggacgc
     3421 gctccgagag ttagctgatg gcagtactga gaggctatgg ccgacgatga tctgttgcta
     3481 gtgccgcccg ttccggacgc gctgcgagaa gcagcgcagg tggccggcaa cctgatcccc
     3541 ttcatcggag caggagcctc ccgtcttgcc gggtgtccga gttggattga actcgctgac
     3601 gcagcgttca actgcttcgt cgatcaaggt aaattcagcc atgcgcagct ggaccaaatt
     3661 aagagtctca acccacgcgt caagctttca ctggccgtcg gcatgcagga cgagctgcga
     3721 atcacgatcg acttcaagaa gctgatacac cccgttgaac gccaagacca tgcgaatggg
     3781 cggcgactgt atggaatcct ggctaaactc ggccgcacat tcgtaaccac gaactgtgac
     3841 gaatggcttg acgaggtgct acctgaacca tccctttccg tacatggagt tgctgatggg
     3901 cgagttggtg ctgtgccgaa gcggcgtgat gtcttcgtcg accgggagga gttgaccgcg
     3961 gcgaatctca atcgcgaggg agcggtcctt cacctccatg ggtccgtgcg aaagccggag
     4021 ggcatgatca tcacgactcg gcattacgtt aagcattatg agaatgaccg acgactgaaa
     4081 accaccgatg ctgagaatcc ggtgctaacg ttccttgagt ttctgttccg cacgaaaacc
     4141 gtcttgttca tcggttacgg gctcgaggag ctggagattc tcgagtacgt cattctaaag
     4201 acgcgtgacc tcaagactcc taatgcgcag ccgcgacact tcatcctgca agggtacttt
     4261 tcgcaccaaa gagaactcat gctcagcatg agacggtatt accgcgattg tgggatcgag
     4321 ctgctgccat tcctgatgga tccaaggggt cacgagcagc tgattgaggt gctcgatgcg
     4381 ttcgcgcgca ccgtgccacc ctcgaccttg gcagtcctgc aggaattcga ggagatggag
     4441 accttactca atggctaacc tcagcgcgaa cgagaagtcg ttcgtcacac tgatgaggaa
     4501 aagcgacgag cacgcccgcc gcggcttcga tctgctcgca aagcgccaag atcctgaaaa
     4561 gttcttcgat gaacttgccg cgcagggtgc gtttagtcca tccgaggctc ccgcgcccgt
     4621 gcgcgtggat gagaattcga ttcgctttcc ccagtggccc gtgctgaatt acttgctcca
     4681 gtgcgcaaga atctcagacg aacagaacga tattgctctc gcgcaaagcg tcatgggcgt
     4741 ggtccgggcc gtgatggcat ttcgggaaga ggacggcaac atacgggata actatgtgac
     4801 gttctggagg ttcgcggaga tacttggtgc tgtgccgaat gccgtcgtca caatcgacga
     4861 tattgagctc gccagcatat ggttgaatag tcggtttgac agaatgatgg tagccagtgc
     4921 cttgagcgaa gggatcttca ggcgttgtct tgcgagtaac gatccggccg attggaacaa
     4981 agccattcag ctgctacctc attgtctggc gatcgtctgg caatcggagc gcggcggcat
     5041 agataaagaa gagccagtga cgatagttga cccttactgg ctaaagaaac taatccgtca
     5101 atacgccgag gagtttggca agaaggttgg cagtgcggcc gccacaatct tccgagagcg
     5161 cgttcaagag ctgtttagta agggtgggcg agtgtcgtgg agttacgtgt ttcgaccggc
     5221 ggttgaggat cacgctcaga accattcgtg gcacgatgct gataactgtt tagttgaggg
     5281 cttgcgcgat gtacttttgg cgtggtcaga catcgaactt gcgggagcca aactgtttgt
     5341 cgacgatctt ctgcgaagca atgtagagat gcttcggcgg atcggcattt acgtcctcga
     5401 tgagcgctgg aaccagcttg gcggccttta cgacgggatt gtggggactg acctcttcgc
     5461 ctccggccac atacacgagt tgtttcattt gttgcggaac cactttgagc agtttgacga
     5521 agagcagaag atggcgaccg tcgccgctat aagagagatt ggcccaccgg aagtggaaga
     5581 tgcggaactc gtgctgaaat ggatccagcg acggtggctg gaggcaatcg ctggaacgac
     5641 ttacgaacct gtagtgactt ggattgcgga gctcgcacgc gatccgcgcg ttagcgttca
     5701 gattcatccc gatttcagca catacatgga agccgcgtgg gggccgggtc caagtccata
     5761 tcaagtcgag gaactcgtgg cgtttgtgcg acatggaacc cttgtcgaga agctgaacgc
     5821 cttcatgcaa cgcccggagt ggcgcggtgt ttcaagcgag gcgctcgtag aggcgctgag
     5881 gcaagcggtg gttgtggcac cggcagcctt cgcacgagca ctgcctccgt ttctggccgc
     5941 caaaccgacg tatcagcatg gcgtgatcgg aggttttaag cgactttggg acgcgccgaa
     6001 ggagcagcag gctagcgcgg actgggagga gatatggctc aacctgttcg atttccttga
     6061 gaagctcttt gaagacccgc aagcagggag aaggagtgaa gtggatgccg gttttgcaca
     6121 gagctggctc gtgtcggaga cagcagacct gcttgatgct ggcacacggg atgacaagca
     6181 cgcgtacccg actgcgctgc tcccgcgggg ctgggcgttg ctcagcatac tcgccgagcg
     6241 cgctgaggct gcggagcagc ctgatgatcg tgatccgatg acgcaggcgt tgaacacgcc
     6301 gaaagggcga actattggcg cgatagtggg ccatgcgcta agggagtcgc gccttgccga
     6361 taagaatggc ggctcgcacg cggctgtttg gaacgggctt cgcccgctgt ttgatcagga
     6421 actcgagaaa tgcgagacgg ggaactacga attcgcaacg ctcgccggcg cttcgatcgc
     6481 taatctggag tacctcgacg cggattggtt gcgggcgcat g
//
LOCUS       DATLVW010000289         2711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_138760, whole genome shotgun sequence.
ACCESSION   DATLVW010000289 DATLVW010000000
VERSION     DATLVW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138760"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VNN25_08960"
     CDS             <1..340
                     /locus_tag="VNN25_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91697.1"
                     /translation="KGIDLLVEASTENVRRLKRAMAVLPDNAIELIADDEIEKYGVVR
                     IADEIVVDLLKSACGVDFARAADGGIETKVVDGVPIPVARKGAADRNETNSSSQRRDR
                     CAVSSTSHRF"
     gene            complement(368..1099)
                     /locus_tag="VNN25_08965"
     CDS             complement(368..1099)
                     /locus_tag="VNN25_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91698.1"
                     /translation="MIRVIIVDDEAPARELLREYLTADDDCEVVAECANGFEAVKAVA
                     QHDPDLLLLDIQMPKLDGFEVLELLDRSPIVVFVTAHDEHALRAFEVHALDYLLKPFS
                     LERFRQVMERVKRTAGHHDRAPVAGIASSLRARPLQRVVVRGEDGAIQVIPVARLDYA
                     EADDDSILIATGGAKLRKQQPIGELAQQLDPARFVRIHRAYLVNIERIEKIELYAKDS
                     RVAILRDGTRLPVSRSGYQRLRELL"
     gene            complement(1096..2136)
                     /locus_tag="VNN25_08970"
     CDS             complement(1096..2136)
                     /locus_tag="VNN25_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXH91699.1"
                     /translation="MQTRKTLTRGTLALYAVGAVTAAATMSSVFFLWTRGAAVGTSIA
                     AALPPSLLLGAIALASRFLCSALPLRSASRMSIAIAHAGSAAAASGLWILTWKSWVPV
                     VNEHVAKTLAPDYSLLFGLAILLYLAAVAVHYLLLEVEAVREAEDEVLRYRVLAREAE
                     LRAFKAQVDPHFLFNSLNAVASMCGSRPLEAREMAQRLADFFRLILRLGALERITLSE
                     EVDLVTRYLEIEKVRFGDRLTVHINVDDAASQCLVPPLLLQPLVENAVRHGVSSMVDG
                     GTVDIVGTLENHMLRIVIDNPADPDRADVRGEGVGLENARGRLSAVSEGRAMLQAKED
                     DGRFHVVIELPR"
     gene            complement(2140..>2711)
                     /locus_tag="VNN25_08975"
     CDS             complement(2140..>2711)
                     /locus_tag="VNN25_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91700.1"
                     /translation="PWWPLVFIALGVAQMPHRQWVSAVALVAIGILFLPPLPILPHFR
                     VSSILAVWPLLISVGGVSLIRQALSPAAKGASRADSFHTVAVMGGVGRAVASSDFAGG
                     DAVAVMGGCEINLGGAKITKEATIDVLAFWGGIDIRVPRGWRIENHVKAILGGVVDKT
                     DDNVPAGAPLLVIRGSAIMGGVEVKHPKR"
BASE COUNT          539 a          840 c          851 g          481 t
ORIGIN      
        1 caagggcatc gacctccttg tcgaggcctc cacggaaaat gtgcggagac tcaagcgagc
       61 gatggcagtt cttcctgaca atgcgatcga gcttattgcc gatgatgaga tcgaaaagta
      121 tggcgtggtg aggatcgcgg atgaaatcgt tgtagatctg ttgaaatcgg cttgtggcgt
      181 cgatttcgct cgcgcggccg acggcgggat cgagaccaaa gtcgtggatg gcgtgccgat
      241 tcctgttgct cgaaaaggag ctgctgatcg aaacgaaaca aacagttcgt cccagcgacg
      301 cgacagatgt gcagtttctt cgacttcgca tcgcttctga gcgcgaggac aactgaccag
      361 gtccgtctca caacaactcc cgcagcctct gatatccgct cctgctcacc ggcagccttg
      421 ttccatcgcg caggatcgcc acgcggctgt ctttcgcgta tagctcgatc ttctcgatgc
      481 gctcgatgtt gacgaggtag gcgcgatgga tgcgcacgaa gcgggcggga tcgagttgct
      541 gggccagctc gccgatcggt tgttgcttgc gcagtttcgc gccgccagtg gcgatcagga
      601 ttgagtcgtc gtcggcttcg gcgtagtcga ggcgcgcgac cggaatgacc tggattgcgc
      661 cgtcctcgcc gcgcacgaca acgcgctgca atggacgggc gcgcagcgag gaagcgatgc
      721 cggcaaccgg agcacggtcg tggtgtccgg ctgttcgctt gacgcgttcc atcacctgac
      781 gaaaccgctc cagcgagaac ggtttgagaa gatagtcgag cgcgtggact tcgaatgccc
      841 gcagtgcgtg ctcatcgtgc gcagtgacga agacgacgat cggcgagcgg tcgagcaact
      901 cgagcacttc gaagccgtcg agcttcggca tctggatgtc gagcaggagc aagtcgggat
      961 cgtgctgcgc gaccgccttc acggcttcga atccattcgc gcactccgcg acgacctcgc
     1021 agtcgtcgtc ggcagtcagg tattcgcgaa gaagctcgcg agctggcgct tcatcgtcga
     1081 cgatgatgac gcggatcatc gcggcaactc aatgacgaca tgaaatcgtc cgtcgtcttc
     1141 tttcgcctga agcatggccc gcccttccga taccgcgctc aatcttcctc gggcgttctc
     1201 cagtccaact ccttcgccgc gcacatcagc gcggtctggg tcggccggat tgtcgatcac
     1261 gatgcgcagc atgtgattct cgagagtacc gacaatatcg accgtgccgc catccaccat
     1321 cgacgagacg ccgtgccgca ccgcattttc taccagcggc tgtagaagta gcggcgggac
     1381 gaggcattgt gacgccgcat catcgacgtt gatgtgaacg gtgagccggt cgccgaaacg
     1441 aaccttctcg atctccaggt agcgcgtgac gagatcgacc tcctcgctga gcgtgatgcg
     1501 ctccagagcg ccaaggcgga ggatcaaccg gaagaaatcg gcgaggcgct gcgccatctc
     1561 gcgggcctcg agcgggcgcg acccgcacat cgaagcgacg gcgttgaggc tgttgaaaag
     1621 aaagtgagga tcgacctgcg ccttgaatgc gcgcaattcg gcctcgcgcg ccagcactcg
     1681 ataacgcagc acttcatcct cggcctcgcg caccgcttcg acctcgagca acagatagtg
     1741 cacagctacg gccgcgaggt agaggaggat cgccaatccg aagaggaggg agtagtcggg
     1801 tgcaagcgtc ttcgcgacgt gctcattgac gaccggcacc cacgacttcc atgtcaagat
     1861 ccacagcccg ctggccgccg cggccgatcc cgcgtgcgca attgcgatcg acattcgtga
     1921 ggcgctccgc agcggcaatg cactgcaaag gaaccgcgat gcgagtgcga tcgcgccaag
     1981 aagaagtgag ggaggaagtg ccgccgcgat cgatgtcccg accgcagccc ctcgggtcca
     2041 caggaagaaa acggagctca tcgtcgcggc tgcggtcacc gcgccgacgg cgtacagggc
     2101 cagtgttccg cgggtcaaag ttttgcgcgt ctgcatcggt tacctcttcg ggtgcttcac
     2161 ttcgacaccg cccatgatcg cgctgccgcg aatgacgagg agtggcgcgc cggcgggaac
     2221 gttgtcatcg gtcttgtcga cgacaccgcc gaggatggct ttgacgtgat tctcgattct
     2281 ccagccccga gggacgcgga tgtcgatgcc gccccagaag gcaaggacgt cgatcgtggc
     2341 ttccttcgtg atcttcgcgc cgccgaggtt gatctcgcat cctcccatca ccgcgacggc
     2401 atcgccgccc gcgaaatcgc tcgacgccac cgcacggccg acgccgccca tcacagcaac
     2461 agtgtggaat gagtccgctc tcgacgcacc cttcgctgcg ggcgaaagcg cctggcggat
     2521 cagcgacacc ccaccgacgc tgatgagcag cggccacaca gcgagaatcg acgacacgcg
     2581 gaagtgcggc aggatgggca gcggcggaag aaacaggatt ccgatcgcga ccagcgccac
     2641 cgctgagacc cactggcggt gtggcatctg cgcgacgccg agagcgatga acacgagcgg
     2701 ccaccatggc a
//
LOCUS       DATLVW010000290        11264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_260285, whole genome shotgun sequence.
ACCESSION   DATLVW010000290 DATLVW010000000
VERSION     DATLVW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260285"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..763)
                     /locus_tag="VNN25_08980"
     CDS             complement(38..763)
                     /locus_tag="VNN25_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HXH91701.1"
                     /translation="MTAAAPATPTVQHPPRVPWIVAFYRSAIVKKAVMAVTGIFLFGW
                     IFLHMLGNLKAYMGPEHYNAYAQFLITMGAPLLPNKAALIAVRTLLFLAAVFHIVAAT
                     QLTIMNRKARPVGYRDREYVAGTYAARTMRWGGVIILLFVWYHIGHLTFGKFHPDFIE
                     GDVYHNFVTGFQVWWVSAIYIIANLALGMHLYHGLWSMFNSLGLNHPGFNPWKRAFAT
                     VFAIVVTAGNVSFPVAVLIGILR"
     gene            complement(1056..2327)
                     /locus_tag="VNN25_08985"
     CDS             complement(1056..2327)
                     /locus_tag="VNN25_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HXH91702.1"
                     /translation="MTLLHFSDYHSHALPFYSEGREGEGGIARAIGYLRREKLRGALV
                     FSGGDMINKGSPAWSDKYRCAEWPWLDGVVDAMALGNHEPDYGFEAMQTCIGSIHFPV
                     LSANTEGFSGSHLFVVNGIRIGVFAIAGNDFRTLVKDPALHFGEPVEAARAAVRALRE
                     TQHADVVVMIGHEHLDDDFALARAVPGIDLIFGTHSHLKRALTRIDGTATWFISPFQY
                     LTYISRVVLTFKGRTLTDVRGRLVPVDAHMPEDKQVAMRVAKMQRDLERDTQYAPLFV
                     SIGSMSAPLSVEALAAKSVAVMRDAANADVALSTASSFRQALPRGNVTVEALRAALPY
                     DNEIFIYSLRGDAVEKLLAYGRSREGSDSVAIVARPASIEPQKTYRVATTDFLAKTAP
                     GYRDFFAGLTPETPGLHVREEVRKWLGRDQR"
     gene            complement(2447..2965)
                     /locus_tag="VNN25_08990"
     CDS             complement(2447..2965)
                     /locus_tag="VNN25_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91703.1"
                     /translation="MPRPRANVSTPSLSAGQAAYVLERLVAERRISAAEVNRCIGDMH
                     REISDLESRLQSLRANAGAAPSAPSAPRRGRPPGSGRGPGRPPGSGRKAAPPAAAPAA
                     AAAPGAAPAPAKRRRRTRITAEQLASRQLQGRYLGLIRQIPASRRAAFQKIAKDRGRE
                     AAINEMRMTLKK"
     gene            3905..4276
                     /locus_tag="VNN25_08995"
     CDS             3905..4276
                     /locus_tag="VNN25_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91704.1"
                     /translation="MNKLRHALVVAVFAAVAVSSISVPAFAASSIKVHNKSKWEIHHL
                     FLSPTSEKDEWGPDQLGDAVIKANGGTFTLTDIECNKYDIKIVDEDGDECIVSAVALC
                     DDSAVWNITDERLLKCEKKSE"
     gene            4359..4772
                     /locus_tag="VNN25_09000"
     CDS             4359..4772
                     /locus_tag="VNN25_09000"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH91705.1"
                     /translation="MKKAKWTVAGLGLLLILAVACTTETAIKSLKLGKDKAVATETSA
                     FATGDTVYAVAAISSSGKVKVTGRLLIDEVEGQTKGPIPGLETTIDLAKGEDGNFTFS
                     PPPAGWPKGKYTLEVVLTDDAGAQKDKKSANFTVS"
     gene            complement(4840..5427)
                     /locus_tag="VNN25_09005"
     CDS             complement(4840..5427)
                     /locus_tag="VNN25_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HXH91706.1"
                     /translation="MLLERKTYVVKEHVAFAKLTATYDLLDAESGAPIGIAKEEPPGW
                     AKWMRLVANKHMLPTAVNVYEGTSTTPLVSIQRGFTFLRARVSVVMGDRTIGHFEAKL
                     MSVGPSFRILDAQDVEIGSVKGDWKGWNFKITSSTGEELGTIAKKWDGLGKALFTNAD
                     TYVIALSAGAAGRPDVAALLLAAGLAVDTVYREKK"
     gene            5573..6094
                     /locus_tag="VNN25_09010"
     CDS             5573..6094
                     /locus_tag="VNN25_09010"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HXH91707.1"
                     /translation="MSEHEDEGKTWTSKASVYLGSAESAVLVLIGVALVMVAVLLLYS
                     GMHDLWEATQGGPKIIEHKSIEILNTVLLVMMTMEIVYTVAISLKSHTLNAEPFLIIG
                     SIAAIRRMLVITATSTETEVNPAIFHNTLVELGLLAATVVAMTIAICILRFSQRKYVD
                     DKIAGAPAIDSHT"
     gene            6167..6532
                     /locus_tag="VNN25_09015"
     CDS             6167..6532
                     /locus_tag="VNN25_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HXH91708.1"
                     /translation="MLMRIIAVGALATLLGTSAFARADRYGAIAYSRRTGHYGYADNA
                     GSREAAERRALERCEGRDCKIEVWFRNSCGALATGEDGQIYGWAHDTSLREAKESAVE
                     HCRHEGGRRCRVLISACTP"
     gene            complement(6597..7784)
                     /locus_tag="VNN25_09020"
     CDS             complement(6597..7784)
                     /locus_tag="VNN25_09020"
                     /inference="COORDINATES: protein motif:HMM:NF019404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HXH91709.1"
                     /translation="MPDSFSSTSSTSWGGNILGSIKSVFFGFILFVVSVPLLFWNEGR
                     AVRTSKGLTEGLGAVVSVPPDSVDPGKEGKLIHTSGAVKTDAPVVDDQLAVQATAVKL
                     TRNVEMFQWKEHEKSESRKKIGGGTETVTTWDYKKEWANGRIDSSSFKHPEGHENPEA
                     PPFETKTFVANKVTVGAFTMSQEQLGQLTNATSLPVDSAALEQLPADVKDRFKVSDGK
                     FYAGDDPATPKVGDVRISFQVVNPSNVSLVGVQSGQSFVPYQTSTGTSILLVEEGTLT
                     AQQMFKKAQDANTFLTWLIRGGGFFLMFIGIFMLFRPIVVFADVLPIFGTMLGAGIGI
                     FAFLGAAVLSFLVIAAAWVIYRPMIGITMIVLAIAALFWLVSVGKKKKAIRAGMVTAP
                     VSV"
     gene            8050..8388
                     /locus_tag="VNN25_09025"
     CDS             8050..8388
                     /locus_tag="VNN25_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91710.1"
                     /translation="MKKTSVKVLLFALLLTTLASISAYAEEYKFKVTNSTKTVITKIL
                     VSEDGAKYGFFDIGKGIKPGQTVELVWDKSTNNEACKQHVKAVYADGSESEPAMFDFC
                     ESDVALEFND"
     gene            complement(8470..9738)
                     /locus_tag="VNN25_09030"
     CDS             complement(8470..9738)
                     /locus_tag="VNN25_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HXH91711.1"
                     /translation="MKETTRVLVALVAALICGIAIAASGNASLLRAVDFIVPVGTLWV
                     NAIRMTVIPLVVSLLITGVASADDLRSIARIGGRSLVTFVALLAGTAVVVIPLALAMF
                     QMLPRRLGIMALPRGAAEAAGDLAGAGQSQTFSAWLSSLLPSNPIAAAASGAMMPLVL
                     FTLLFALAVARTPDPARATLLGFFRALGDAMLTLVRWVVMLAPLGVFALVLPLAVHAG
                     AELAGGIGFYIAVYSAGNLIVTLLLYPVVALAARISMRRFAKAALPAQLIAFSSSSSI
                     ASLPALVESAERGLELPNRVTGFVLPLAVSTFKIAAPLSWTVGALFIGWFYGVPLHAT
                     AIATIAFAAVFLSFAVPGIPRGAFIMLTPLFLAIGLPVEGIGILIAVDAIPDTFSTVL
                     NVTGDLAATALVARGEEESAVIESEQTPTA"
     gene            complement(9760..10503)
                     /locus_tag="VNN25_09035"
     CDS             complement(9760..10503)
                     /locus_tag="VNN25_09035"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXH91712.1"
                     /translation="MNDFEGRVAIVTGAARGLGRAAAERLYERGAAVAVNVRDRARAE
                     SLAQSLGERALAVPGDIAAPGIPEEIVRATVERFGRVDILVNNAAFARSTRFPSLSAE
                     EWREALEVNLTAPFLFTKAVLPIMKAQNYGRIINVSSSAGRTVSTLGGAHYTASKAGL
                     LGLTRASAKELGQFGITVNAVCPGMIDTELTHENASSELLTRLAASYPVPRLGTSLEV
                     ADLICFAASEAAGYITGASFDINGGDLMM"
     gene            10624..>11264
                     /locus_tag="VNN25_09040"
     CDS             10624..>11264
                     /locus_tag="VNN25_09040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH91713.1"
                     /translation="MIDQIGPYRIVRKLGEGGMGVVYAAEDERLHRAVAIKTIRDNGD
                     ASARERFLREARAAASLSHPNVCQLFDIGDTEGTPFLVMELLDGESLGERLTRGALPL
                     AESIRITLAILTALEALHGRGFVHRDLKPSNVFLTGYGVKLLDFGLARELQPAQLSAD
                     ATTMQPGHSPLTLSGMIVGTPRYMAPEQILGAPVDGRTDLFSAATLLYEMVAGA"
BASE COUNT         2321 a         3526 c         3372 g         2045 t
ORIGIN      
        1 gttccctgcc tgtgtggttt tatgaatgac gctggggtca acgcaggatt ccaatcagga
       61 cggcaaccgg aaaggagacg ttgccggccg tgacgacgat cgcgaaaacg gtcgcgaagg
      121 cgcgcttcca gggattgaat cccgggtggt tgagtcccag cgaattgaac atgctccaga
      181 ggccgtggta gaggtgcatt cccagcgcaa ggttggcaat gatgtagatc gcgctcaccc
      241 accacacctg aaagccggtg acgaagttgt ggtagacgtc gccttcgatg aaatcgggat
      301 ggaacttgcc gaaggtcagg tgtccgatgt ggtaccagac gaagagcagg atgatgacgc
      361 cgccccaccg catcgtccgc gccgcatagg tcccggcgac gtactcacga tcccgatagc
      421 cgacgggccg cgccttgcga ttcatgatcg tcaactgcgt agcggcgacg atgtgaaata
      481 cggccgccag gaacagcaga gtgcgaacgg cgatgagtgc cgctttgttc ggcagcagcg
      541 gcgcccccat cgtgatcagg aactgcgcgt aggcgttgta atgctcgggg cccatgtagg
      601 ccttcagatt gccgagcatg tgcaggaaga tccagccgaa gaggaagatg cccgtaaccg
      661 ccatgacggc tttcttgacg atggctgagc ggtagaaggc gacgatccag gggacgcgcg
      721 gcggatgctg aacggtcggt gtcgcgggag cggcagcggt catggctgca tcatatcgat
      781 gaagcgcgtc gggtcaagga tgagggatca ctcgtggtgg tgaggacaga gatcaggagg
      841 cagagacgag agatcagaga tcaggactcg gcttcccctg atctcctgtc tctgatctct
      901 atccttctca ggagaggaga tcgggaagct gggatcagag atcagagatc atgactccgg
      961 cttcccctga tctcttgtct ctgatctctg tcttctcatg acgtagagat cattaagaag
     1021 agaccggaga tcaggcctgc gcctcccctg atctcttatc tctgatctct gcctaaccac
     1081 ttcctcactt cttcccgcac gtgcagcccc ggagtctcgg gagtcaggcc ggcgaagaaa
     1141 tcgcgatacc cgggtgcagt cttcgcgaga aagtcggtcg tcgctacgcg gtacgtcttc
     1201 tgcggctcga tcgaggcggg tctggcgacg atcgctaccg aatcgcttcc ctcgcgggag
     1261 cggccgtagg cgagcaactt ctcgacggca tcgccgcgaa gtgagtagat gaaaatctcg
     1321 ttgtcgtatg gcagggccgc gcgcaatgct tcgaccgtca cgttgccgcg tggaagagcc
     1381 tgccggaaac tgctggctgt cgaaagagcg acatccgcat tcgcggcatc gcgcatgacc
     1441 gctactgatt tcgcggcaag ggcctcgacg ctcagcggtg cggacatact cccgatgctg
     1501 acgaagagcg gcgcgtattg tgtatcgcgc tccagatcgc gttgcatctt cgccacgcgc
     1561 atcgccacct gcttatcctc gggcatgtgc gcatcgaccg gaacgagccg gccgcgtacg
     1621 tccgtcagcg tccgtccctt gaacgtgagg accacgcggc tgatgtacgt cagatactga
     1681 aacggcgaga tgaaccacgt cgccgtgccg tcgatgcgcg tcaacgcgcg cttcaaatga
     1741 ctgtgtgtac cgaaaatcag gtcgatgccg ggtaccgcgc gggccagcgc gaaatcgtcg
     1801 tcgagatgct catgaccgat catgacgacg acgtcggcat gctgcgtttc ccgcagcgcg
     1861 cgcaccgccg cgcgggccgc ttcgaccggc tctccgaaat gaagcgcagg atctttcacc
     1921 agcgtacgga aatcgtttcc ggcaatggca aagacgccga tgcggatacc gttcacgacg
     1981 aaaagatggg agccactgaa tccctctgta ttggcgctga gcaccgggaa atggatcgat
     2041 ccgatgcatg tctgcatggc ctcgaatccg tagtcaggct cgtggttgcc taatgccatc
     2101 gcatcgacca cgccgtccaa ccacggccac tccgcgcagc ggtacttatc ggaccaggcc
     2161 ggcgatcctt tgttgatcat gtcgccgccg gaaaagacga gcgcgccgcg aagtttctcg
     2221 cgccggaggt acccgatcgc cctggcgatc ccgccctccc cttcccgccc ctcggagtag
     2281 aagggcagcg cgtgcgagtg atagtcggag aaatggagga gggtcacgtg cgtcgtatgc
     2341 acagaagcct gcacctggtc gggcaggcaa gcgcaaagga cgagcagcgc gaaaagaaaa
     2401 gggcgccctg acaggcgccc ttcactacga attactgacg gagcgctcac ttcttgagcg
     2461 tcattctcat ttcgttaatt gctgcttcgc gtccgcgatc cttggcgatc ttctggaacg
     2521 cagcccggcg gcttgccgga atctggcgga tcaggccgag ataacggccc tgcaactgac
     2581 gcgatgccaa ttgctcggca gtgatgcggg tacggcggcg gcgcttggcc ggagccggtg
     2641 cagcgcctgg cgctgcggca gccgcgggtg cggcggcagg cggcgcggct ttacgtccac
     2701 ttcccggcgg acggccagga ccacggccac ttccgggtgg acggccgcgg cggggagctg
     2761 aaggtgcgct cggcgcagct ccggcattcg cgcgcagcga ctgcagacgt gactcgagat
     2821 cactgatctc acgatgcata tcgccgatgc aacgattgac ttctgcggca gagatccgcc
     2881 gttcggcaac taaccgttcc aatacatagg cggcttgtcc ggcggacaac gagggggttg
     2941 agacattcgc tcttggtcta ggcacgttat tcactccttt tgttgtgctg agtgcacttt
     3001 gatttgtgca tgctaccaga acttccctat gacggcgcaa tacaaatcac attgtaagcc
     3061 tcttgaatga acttgttatt ggtcaaaaat ggaccccctt ggctgaatgg cagtaaccaa
     3121 aagcggtggg gaggagcaag gctgatcctg caaaaccgga gcagcgtgcg cgccctatac
     3181 gagtcgccgt taatcgaggt tagggggtgg ccccgacccc ggcaatcggc gcatctcacc
     3241 cttgtaggga atagcggcgg cgcactccca tcgagcgcct tctcccccac gctttttgtg
     3301 ggggagaagg tggccgaagg ccggatgagg gggcgtctga ctgtcgcggc acagttgaaa
     3361 aacaaactac caatctggtt gctgcgatcc ttttgcagcc cccctcatcc gccttcggca
     3421 ccttctcccc ccgaaaaggc ccggggggag aaggcgctcg attggaaggg agtcgctggc
     3481 aatggagaga gcgctccgcg ggtgtcgcaa gcccgaaatc tcaacataag ataaacgtcc
     3541 gatagcccct cccagacggg aggattacca tcacaaagcc cgtgactttc accccggaac
     3601 cgcgtatcgt cagcgcccgt cgaagcgtcc tcgaccaatc aggaaaatgc cagggcactc
     3661 agacccactc attcctcgct gccgcatgcg tgaacccatc ccgctcgccg atcgagatcg
     3721 cggatctctg cttcatggtg aagggcgctc gctgttccct gtcgacgcgc cggcgttgac
     3781 gaacgggact ggcggcaaca aacaaacaat cgaatctgcg gtgtggggcg gcagcaatgt
     3841 tgcttccgct tctcccgcga taaacctaaa tctggtcgat gcctcatcga caacaaggag
     3901 tctcatgaac aagctacgtc acgcacttgt cgtcgcagtc tttgccgccg ttgcggtttc
     3961 ctccatctcc gtccccgcct ttgccgcctc cagcatcaag gttcacaaca agagcaagtg
     4021 ggagattcac cacctgtttc tctcgccgac cagcgagaag gatgaatggg gtcccgacca
     4081 gctcggcgat gccgtgatca aagcgaacgg gggcaccttc accctgaccg acatcgaatg
     4141 caataagtac gacatcaaga tcgtggacga agacggtgac gaatgcatcg tgtccgctgt
     4201 tgcgctgtgc gatgactcag cggtctggaa catcaccgac gaacggctgt tgaagtgcga
     4261 aaagaagtct gagtaacgaa caagagcggc gcgcaacctg catgccctga ctaacacaca
     4321 gcggacccga ggccgaacct tcgggaaggg gaacaagaat gaaaaaagca aaatggacag
     4381 tggccggcct gggtcttctt ctgattctgg ccgttgcgtg cacgacggaa acggccatca
     4441 agtcgctgaa gctcggcaag gacaaggcag tcgccaccga gacctccgcg tttgcaaccg
     4501 gcgacacggt ttacgcggtc gccgccatct ccagttcggg caaggtcaaa gtcacaggcc
     4561 gcctgctcat cgacgaagtg gaagggcaga cgaagggtcc gatcccgggc ctcgagacca
     4621 cgatcgatct ggccaaaggc gaggacggga atttcacctt ctcacctccg cctgcaggct
     4681 ggccgaaggg caagtacacg ctggaagtcg tgctgaccga cgacgccggc gcccagaagg
     4741 acaagaagtc cgcgaacttc acggtttcct aggccgccaa aacttcggag ggcgggcacg
     4801 cgcccgccct ccatttgcgt ttcttacttc ttctcgcgct tacttcttct cacgatagac
     4861 ggtatcgacc gccagtcccg ccgcaagcag gagcgcggcg acatccggcc gccccgcggc
     4921 cccggccgag agcgcgatca cgtaggtatc ggcattcgtg aacagcgcct tccccagccc
     4981 gtcccatttc ttcgcgatcg ttcccagctc ttcgccggtg gagctcgtaa tcttgaagtt
     5041 ccaccctttc cagtcgccct tcaccgatcc gatctccaca tcctgcgcgt cgagaatccg
     5101 gaaggaagga ccgaccgaca tcagcttcgc ttcgaagtgt ccgatcgtgc ggtcgcccat
     5161 gaccacggag actctggcgc ggagaaacgt gaagccgcgc tggatcgaga ccaacggcgt
     5221 cgtgctcgtt ccttcgtaga cattgacggc tgtcggcagc atgtgcttgt tggcgacgag
     5281 ccgcatccac ttggcccagc ctggcggctc ttctttggca atgccgatcg gtgctccgct
     5341 ctcggcatcg agaaggtcgt aggtggcagt cagcttggca aacgcgacgt gctccttgac
     5401 gacgtacgtt tttcgttcca ataacataag actctctccc tgatgttgtt ttttcgtgcg
     5461 cgttcagcct accacggtcg atttgatcct caccccgggc gtttcagacc gcctggccgg
     5521 aagcgatatt cgttgattgc ggcagccttc gatcggccta tactgctgag ctatgagcga
     5581 acacgaagac gaaggaaaaa cttggacctc caaggcgtca gtctacctgg gctccgccga
     5641 gagcgcggtt ctggtgttga tcggagtcgc gctggtgatg gtggccgtgc tgctccttta
     5701 ctcgggcatg catgacctgt gggaagcgac gcagggcggg ccgaagatca tcgagcacaa
     5761 gtcgatcgag atcctgaaca ccgtgctcct cgtgatgatg acgatggaga tcgtctacac
     5821 ggttgccatc tcgctcaaat cgcacacgct gaatgccgag cccttcctga tcatcggctc
     5881 gatcgccgcg atccggcgca tgctggtcat cacggccaca tcgacggaga ccgaggtcaa
     5941 cccggccatc tttcacaaca cgctcgtgga gctgggactc cttgccgcta cggtggtggc
     6001 gatgacgatt gccatctgca tcctccgttt cagccagagg aagtacgtcg acgacaagat
     6061 cgccggcgca cctgccatcg attcccacac ctgatccgca ggccttcaag gcgcccggat
     6121 gacccgggcg atcctcgagt ctttgaacga gaaaagaggg acaaccatgt tgatgcgaat
     6181 cattgctgtt ggtgctcttg caactctcct gggaacatcg gccttcgctc gcgccgatcg
     6241 ctatggcgcg atcgcctact cccgccgcac aggccactac ggctacgcgg ataacgccgg
     6301 cagccgcgag gccgcagaga gacgcgctct cgaacggtgc gaaggacgcg actgcaaaat
     6361 cgaggtctgg ttccgcaata gctgcggagc tctggccacg ggggaggacg ggcagatcta
     6421 cggctgggcc catgacacga gcctccgcga ggcgaaggaa agcgcggtcg aacattgccg
     6481 ccatgaaggc ggccgccgct gccgagtctt gatctcggcc tgcacgccgt aaagaaccga
     6541 agagtgaatg gtgaagagtg aaacactctt caccattcac aggtttgcgt taacgctcag
     6601 accgagacag gcgcggtgac catccccgcg cgtattgctt tcttcttctt cccgacgctc
     6661 accagccaga agagcgctgc gatggcgagg acgatcatcg tgatgccgat catcggacgg
     6721 tagatcaccc acgccgccgc gatcaccagg aacgacagaa ccgccgcgcc gaggaaggcg
     6781 aagattccga tccccgctcc cagcatcgtc ccgaagatcg gcagcacgtc ggcgaatacg
     6841 acgatcggcc ggaacagcat gaagatgccg atgaacatca ggaagaaccc gccaccgcgg
     6901 atcagccacg tcaggaacgt gttggcgtcc tgcgccttct tgaacatctg ctgcgccgtg
     6961 agcgtcccct cttccacgag cagaatcgag gttccggtcg aggtctgata cggaacgaag
     7021 ctctgtccgc tctggacgcc gacgagactg acgttggagg gattcacgac ctggaacgag
     7081 atccgtacat cgccgacctt cggcgtggcc ggatcgtcgc ctgcgtagaa cttcccatcg
     7141 gacaccttga aacgatcctt tacgtcggcc ggcagttgct ccaacgccgc ggagtcgacc
     7201 ggcaggctcg tcgcgttcgt cagctgcccg agttgttcct gcgacatcgt gaacgcgccg
     7261 accgtcactt tgttcgcgac gaaggtcttg gtctcgaacg gcggagcctc cggattctcg
     7321 tgcccttccg gatgcttgaa gctggaggag tcgatccgcc cgtttgccca ctctttcttg
     7381 tagtcccacg tcgtcacggt ctcggtgccg ccgccgattt tcttccgcga ctcgctcttc
     7441 tcgtgttctt tccactggaa catctcgacg ttgcgggtca gcttcaccgc agtcgcctgc
     7501 acggccagtt ggtcatcgac gaccggggcg tcggtcttca cggcgccgct cgtatgaatg
     7561 agcttccctt ccttgccggg atcgacggaa tccggcggca ccgatacgac cgccccgagt
     7621 ccctcggtca gccctttcga cgtgcggacg gcccttcctt cgttccagaa cagaagcgga
     7681 accgagacca cgaacaggat gaaaccgaaa aacacgctct tgatcgatcc gagaatgttg
     7741 ccgccccacg atgtgctgga cgtggaggaa aaggaatcag gcatggcgcg atctcctcta
     7801 aagtactggt tggatttgga aggcggacga ggcccgtgcg cgccactcta cgctgaacaa
     7861 tcgtgggacg ctacagcgcc cttcggtcgg tcataagaat catggggaac ccatatgact
     7921 gagactgact ccatggatct ctcaaatcct tattgcgtac ccgttccaac agttatacta
     7981 ttaattgatc gtccacgccg ggacccaccc ggtgcgcatt taatccaata caagagagag
     8041 ataacgttca tgaagaaaac gtctgtcaaa gtactgcttt tcgccctcct cctgaccacc
     8101 ctggcctcga tctccgcgta cgccgaggaa tacaagttca aggtcaccaa cagcacgaag
     8161 accgtcatca ccaagatcct tgtttccgaa gacggcgcga agtacggctt cttcgacatc
     8221 ggcaaaggca tcaagccagg ccagaccgtc gagctcgtgt gggacaagtc gaccaacaac
     8281 gaagcttgca agcagcacgt gaaggccgtc tacgcagacg gttccgagtc cgagccggcc
     8341 atgttcgatt tctgcgaatc ggacgtcgct ctcgaattca acgactaacg tttctcgaat
     8401 cgcgaatccg cggcgggacc actctcacgg gtggtcccgc tttgtttttc cggacaagtc
     8461 cggaacccgt caagcggtcg gggtctgttc actctcgatc accgcgctct cctcctcccc
     8521 tcgggccacc agcgcggtcg cggcaagatc gccggtcaca ttgagaacgg tcgagaaggt
     8581 atccggaatc gcgtccacgg cgatgaggat cccgatccct tcgaccggca gaccgatggc
     8641 cagaaacaac ggtgtgagca tgatgaaggc gccccgagga atgcccggta ccgcgaatga
     8701 caggaagacg gcggcgaacg cgatcgtggc gatcgccgtc gcgtgcaatg ggacgccgta
     8761 gaaccagccg atgaagagcg cgcccacggt ccacgacagc ggagcggcga tcttgaatgt
     8821 ggaaacggcc aacggcagca cgaatccggt gacacgattc ggaagctcga gtccgcgctc
     8881 cgcactttcg acgagagcag gaagggatgc gatggacgag ctcgagctga atgcgatgag
     8941 ctgcgccggc agtgccgctt tggcaaagcg ccgcatcgaa atccgagccg ccagcgcgac
     9001 tacgggataa agcagcagcg tcacgatgag attgccggcc gaatacacgg cgatgtagaa
     9061 accgatgccg ccagccagtt ccgctcccgc gtgaaccgcg agcggcagca ccaacgcgaa
     9121 gacgccgagc ggcgcgagca tcaccaccca ccgcaccaga gtgagcatgg catcgccgag
     9181 cgcgcgaaag aatccgagca gcgtggcgcg cgccggatcg ggcgtgcggg ccaccgcaag
     9241 agcaaagagg agcgtgaaga gaaccagcgg catcatcgcg cccgacgccg cggcagcgat
     9301 cggattcgaa ggaagcaacg acgacagcca cgccgaaaac gtctggctct gtccggctcc
     9361 cgccagatcg ccggccgcct ccgctgcgcc tcggggtagc gccatgattc cgagccggcg
     9421 cggaagcatc tgaaacatcg ccagcgcgag cggaatcact acgaccgccg ttccggccag
     9481 gagtgcaacg aacgtaacca gcgatcgacc gccgatgcgc gcaatcgacc ggagatcatc
     9541 cgcggacgcg acgcccgtaa tgagcaacga cacgaccagg gggatcacgg tcatgcggat
     9601 cgcgttcacc cacaacgtcc ccacaggcac gatgaaatcg acagcccgga gcagcgaagc
     9661 gttccccgaa gcagcgatgg caatcccgca gatcagcgca gcaaccaggg cgacgaggac
     9721 acgtgtggtt tctttcaacg gagcgacctc ctgcaaacct cacatcatca aatccccgcc
     9781 attgatatcg aacgatgccc cggtgatgta ccccgccgcc tcggaggcag caaagcagat
     9841 caagtccgcg acctccagcg acgtcccgag ccgcgggacc ggataactgg cagctagtcg
     9901 cgtcagcaac tcggagctcg cgttctcgtg cgtcagctcc gtgtcgatca ttcccggaca
     9961 aaccgcgttc actgtgatgc cgaactgtcc gagctccttc gcgctcgcgc gggtaaggcc
    10021 gaggagtccg gctttcgatg cggtgtagtg cgcgccgccg agcgtgctca cagtgcggcc
    10081 ggcggaggac gagacattga tgatgcgccc gtagttctgc gctttcatga tcggcagcac
    10141 ggccttcgtg aagaggaacg gcgcggtgag gttcacctcc agcgcttccc gccactcctc
    10201 ggccgaaaga ctcggaaagc gcgtcgatcg tgcgaaggca gcattgttca caaggatatc
    10261 gacccgcccg aaccgttcga cggtcgcgcg gacgatctcc tccggaatac ccggcgctgc
    10321 gatgtcgcct ggaactgcga gtgcgcgctc gccgagcgac tgcgccagcg attccgcccg
    10381 cgcacgatcg cgcacattca ccgcgacggc cgcgccccgc tcataaagac gttcggccgc
    10441 cgcgcgtccc aatccacgcg ccgcaccggt gacgatggcg acacggcctt cgaagtcgtt
    10501 catgacttcg cacatcttga cacgccacga tccgacctcc gattagaaaa ccggtcacca
    10561 cagcggcaca gagttctcag agaccacgat atgacaggag gaagaggcga taatgcctct
    10621 ccgatgatcg atcagatcgg gccgtatcgc atcgtgcgca agctcggtga gggtgggatg
    10681 ggcgtcgttt acgccgcgga agacgagcgg ctgcatcgtg ccgtcgccat caagaccatt
    10741 cgcgataatg gcgacgcatc ggcgcgtgag cgcttcctgc gcgaggcgcg cgcggcggcc
    10801 agtctgtcgc atccgaacgt ctgccagctc ttcgacatcg gcgacaccga ggggacgccg
    10861 ttcctggtga tggagctgct cgacggcgag tcgctcggcg agcgcctgac ccgcggcgca
    10921 ctcccgctcg ctgagtccat ccgcatcacg ctggcgatac tcaccgcgct cgaagcgttg
    10981 cacggccgcg gcttcgtgca tcgcgatctc aagccgtcga acgtcttcct taccggctac
    11041 ggcgtcaagc tcctcgactt cgggctcgcg cgcgagctgc agccggcgca actctccgcc
    11101 gatgcgacga cgatgcagcc cggccactcg cctctcacgc tctcgggcat gatcgtaggc
    11161 acgccgcgct acatggcgcc cgagcaaatc ctcggcgcgc cggtcgatgg ccgcaccgat
    11221 ctcttctccg ccgcgacgct gctttacgag atggtcgccg gcgc
//
LOCUS       DATLVW010000291         3968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_260394, whole genome shotgun sequence.
ACCESSION   DATLVW010000291 DATLVW010000000
VERSION     DATLVW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260394"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1700)
                     /locus_tag="VNN25_09045"
     CDS             complement(357..1700)
                     /locus_tag="VNN25_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91714.1"
                     /translation="MRRIGAAAIFLFTSLPLFGQTPSDGQASSDWTFKTRIELRANYR
                     DSKEEKFALKFPFQPIQLPVGQTTAFEETVDAGAHTELSVAQLKLDLGYGNWFLAHTQ
                     LHALDKYRRNPTSADRKIDADELWIQFGPRPEFLDRPERTSFFVQMGKFVKMERQPIR
                     VLESYGLAATSFNRFEDVGVMTGGSVGRNFYWRLTGTTGNPLYIRDPNALAGDNGINE
                     LLQPHPDPRLKSGFPILYDTRVEDLALDTKNKSFGQGLGYRWQNDAQTFGFDAVAFHY
                     RRKLRDDESAPLTGTFYGVDLDLLEGPLDRGLPIRGNKKEETGARIYTELKGLTSTIQ
                     FTSQSVAGMHRSGAEAEFGYKFGWSGGPSVGGESLLQSVQPAVRWSELHYGFKGNGTQ
                     YPAPSIWWNWTKIDAGIRIGFAKNVDLTIERARNNIGAPKKIHPDETLATVRIKI"
     gene            complement(1850..3769)
                     /locus_tag="VNN25_09050"
     CDS             complement(1850..3769)
                     /locus_tag="VNN25_09050"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH91715.1"
                     /translation="MVKLPTVDRVISLVRKPGQPLPSAAVSKTRESRLRGAFDHAPVG
                     IALASMHGTWLETNERFCELIGYTRDELARMTFAELTHPDDARTEERLMRRLLAGEVP
                     AYRIEKRIMGKKGRHRELEVVCAISGSDDAPFLIYIVDHRSRAAPEQGQVETPVHART
                     ASHNAELLLAVIDRLPDIAIIRTDDKGLIAGWNAGAERILGYKRDEIIGRPRRMLYRD
                     NEGWAGVSTQQLNAAASGSLDLEDWRVRKDGSEVWVRTSITAVRIAGSVKGFVEVIRT
                     PERPEPKTAALKQSVESMQAELRKRERTEDSLRSAVEELRLTGEQTMDELKIMTDALR
                     KELERRKRAEEELRAIHERASRTVVLPPAPAESEIVSTSMPPPRKWKSLGDGNPAELL
                     VKHISHERTGRLVVASGAREKELFVDKGKIISCSSNDPSRFLAQRLIGSGLISEEQRQ
                     KALEIQSETRLALGRILVILGALTENQLHDAMRSKAEEEIAELCGWRDAKFVFVEEEI
                     PTLQLVPLRIDAAELLVHQVVQQIDHRAAPAPATSHGLEAIDVARIVDDAVTDLVIDD
                     IASAADEIAFNIEAISSKAEILIASASGKTKRFHRASCGSAKRLADETRVVFMSAQEA
                     MNAGYDACRMCFRDW"
BASE COUNT          654 a         1228 c         1270 g          816 t
ORIGIN      
        1 tttgcccctg aaagtgacgt gagaccgcga aatttgctcc cgtttcgaac cggcattgcc
       61 ggagacaaat ttctcggtcg atctggtggt tcccacctga tctgatcatg tgacggatca
      121 ggcggcaaaa ggttacgcga ccgatagtag taggagagat gagaggccac tacatgaccg
      181 attcagggcg taatgtccgg caatcgtaac aagcggttgc tcgtgatcac cgttgccgag
      241 tctggtgagg atgttgtcga gctcggcgag actctgcatc ttccgacacg aacgccgggg
      301 ctcaccgtcg ggcgggacgc ccgccggccg caggcgagac gcctgcactc cttgtttcaa
      361 atcttgatgc ggaccgtggc cagcgtttcg tcgggatgga tcttctttgg cgcgccgatg
      421 ttgttccggg cgcgctcgat ggtgaggtcg acgttctttg cgaaaccgat gcggatgccg
      481 gcgtcgatct tcgtccagtt ccaccagatg ctgggcgccg gatactgcgt gccgtttccc
      541 ttgaagccgt agtgcagctc gctccagcgc acggcgggct ggacgctctg cagcagcgac
      601 tcgccgccga ccgaggggcc acccgaccat ccgaatttgt acccgaactc tgcctctgcg
      661 ccgctgcggt gcatgcccgc caccgactgg ctggtgaact ggatcgtgga ggtcaggccc
      721 ttcaactcgg tgtagatacg cgcgccggtc tcctccttct tattgccgcg aatcggcaga
      781 ccgcggtcca gcgggccttc gagcaagtcg agatcgacac cgtagaacgt tccggtcagc
      841 ggcgcgctct catcgtcgcg caacttgcgg cggtagtgaa acgcgaccgc gtcgaacccg
      901 aatgtctgcg cgtcgttttg ccagcggtat cccaggcctt gcccgaatga cttgttcttg
      961 gtgtccagcg ccaggtcctc gacacgcgtg tcgtacagga tcgggaatcc ggacttcaag
     1021 cgcggatcgg gatgcggctg cagcagttca ttgatcccgt tgtcgcccgc gagcgcgttc
     1081 ggatcacgga tgtagagtgg attgccggtc gtgccggtga ggcgccagta aaaattgcgc
     1141 ccgaccgaac cgccggtcat gacgccgaca tcctcgaacc ggttgaagga cgtggccgcc
     1201 agtccgtagc tttccagcac gcggatcggc tggcgttcca tcttcacaaa cttgcccatc
     1261 tggacgaaaa acgacgtccg ctcggggcgg tccaggaact ccggccgcgg tccgaactgg
     1321 atccacagct cgtcggcgtc gatcttccgg tcggcgctgg ttggattgcg gcggtatttg
     1381 tccagtgcgt gcaactgcgt atgcgcaagg aaccagttgc cgtagccgag gtcgagcttg
     1441 agctgcgcga cgctgagctc cgtgtgcgca ccagcatcca cggtctcttc gaaggcggtc
     1501 gtctgcccga ccggtagctg aatcggctgg aagggaaact tgagcgcgaa cttctcctct
     1561 ttgctgtcgc ggtagttcgc ccgcaactcg atccgcgtct tgaacgtcca gtcgctactg
     1621 gcctgtccgt cgctgggagt ctgtccgaaa agaggaaggg aagtgaataa aaagatggcc
     1681 gccgcgccga ttcgtcgcat gtgttctcca gtcttcgtcg cggggagccg gacgtgtgga
     1741 ttgtagcagg ggacttcctg tttccaggcg gacaaacctt taaccacaga gacacgaagg
     1801 acacggagac ctctgtgctc tctgcgcctc tgtggttaag ggttctggat caccaatccc
     1861 gaaaacacat ccggcacgcg tcgtatcccg cgttcatcgc ttcctgggcg ctcatgaaca
     1921 cgacgcgcgt ttcgtcggcg aggcgcttgg cgctgccgca gcttgcgcgg tgaaagcgct
     1981 tggttttgcc gctggcgctg gcgatcagga tctcggcttt cgaggagatc gcttcgatgt
     2041 tgaatgcgat ctcgtcggcg gcggaggcga tgtcgtcgat gaccaggtcg gtgacggcgt
     2101 cgtcgacgat gcgcgcgacg tcgatcgctt ccagcccatg cgacgttgcc ggagccggcg
     2161 ccgcgcgatg gtcgatctgc tgcacgacct gatgaacgag gagctcggcg gcgtcgatgc
     2221 ggagcgggac gagctgcaag gtcggaatct cttcctcaac gaacacgaac ttcgcgtcgc
     2281 gccacccaca gagctcggcg atctcctcct ccgctttgct gcgcatagca tcgtgaagct
     2341 ggttctcggt cagggcgccg aggatcacca ggatgcggcc gagtgcgagg cgcgtctcgc
     2401 tctggatctc cagcgctttc tgcctctgct cctcggaaat caatccgctg ccgatgagcc
     2461 gctgcgccag aaagcgcgat ggatcgttcg aggaacagga aatgatcttt cccttgtcga
     2521 cgaacagctc tttctcgcgc gcaccgctgg ccacgacaag ccggccggta cgctcgtgcg
     2581 agatgtgttt gacgagcagc tccgcgggat tcccgtcgcc gagcgatttc cactttcgcg
     2641 gcgggggcat cgatgtcgat acgatctccg actccgccgg cgccggcggc aggacgacgg
     2701 ttcgcgaggc gcgctcatgg atcgcgcgta gctcctcttc cgcccttttc cgccgctcga
     2761 gctccttgcg aagggcgtcg gtcatgattt tgagctcgtc catcgtctgc tcgccggtga
     2821 gtcgcagctc ttcgacagct gagcgaaggg aatcctcggt acgctcgcgt ttgcgaagct
     2881 ccgcctgcat cgactccacg ctctgcttca acgcagccgt cttcggttcc ggacgctccg
     2941 gcgttcggat tacttcgacg aaacctttga cgctgccggc gatgcgcacc gcggtgatgc
     3001 tggtgcggac ccagacctcg ctaccgtcct tgcgcacgcg ccagtcttcg aggtcgaggc
     3061 tgcccgacgc tgcggcgttg agttgctgcg tggagacgcc ggcccaccct tcattgtcgc
     3121 gatacagcat ccgcctcggg cgtccgatga tctcgtcgcg cttgtatccg agaatgcgct
     3181 ccgcaccagc attccagcca gcgatgagcc ccttgtcatc ggtgcgaatg atggcgatgt
     3241 cgggcagccg gtcgatgacc gcgagaagaa gctcggcgtt atgcgacgct gtgcgggcat
     3301 ggaccggagt ctccacctgt ccctgctccg gtgccgcacg cgaacgatga tcgacgatgt
     3361 aaatgaggaa cggagcgtcg tcgcttcctg agatggcgca gacgacctca agctcgcgat
     3421 gtctcccctt ctttcccatg atccgtttct cgatgcggta ggctggcact tcgccggcga
     3481 gcagccgccg catcagacgc tcctcggtcc gcgcatcgtc ggggtgcgtg agctcggcaa
     3541 acgtcatgcg cgccagttcg tcgcgcgtgt agccgatcag ctcgcagaat cgctcgttcg
     3601 tttccagcca cgtgccgtgc atcgaggcga gggcgattcc gaccggcgca tgatcgaacg
     3661 cgccgcgcag acgcgactcc cgcgtcttcg agaccgcggc cgaaggcagc ggttgcccag
     3721 gtttgcgcac gagcgagatg actcgatcga ccgtcgggag cttgaccatg ttgaattaag
     3781 ggagtcggga aagggtagca cggggtgggt gtggggggtg gggagtgggg ggtgggagat
     3841 ctggttgtcg gttgtcggtt gtcggggatc gggggtcgga gatacttcgg catgaaccgc
     3901 gcgctgtccg gcttcccttc gcgtcttcgc gtcttcgcgt gaccggtact cccccccccc
     3961 ccaccccc
//
LOCUS       DATLVW010000292         3911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_301158, whole genome shotgun sequence.
ACCESSION   DATLVW010000292 DATLVW010000000
VERSION     DATLVW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301158"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2366
                     /locus_tag="VNN25_09055"
     CDS             <1..2366
                     /locus_tag="VNN25_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91716.1"
                     /translation="GNFGVPISVSVVASGAVSYQWYRAASGDPSNPVSGGTGPVLTVT
                     PQATGFTDYWVKVTNACGSTNSSTVTITINCAPRTMSVRTPPAVLSGSTYTVSWNFDP
                     QLDVRNELQEATKADFSDAVNFEVTGVGSRTFTHTVTADTRYYYRVANAPLCGGLGSY
                     GQAGSTLVTAPQAATQANFDFAQAPCTGPNCAVRQSLFIPGFVTAGKTALALGDTYSV
                     SSDESFVTVSPSSGALPAEGVTVTVTIDTSQLDFGATQASVTVIRTPAPGKTGALGDP
                     PATNTVPISVSVVAPVTPVPKDPNAPFNALLIPAIAHADGIGTRFVSDVRLTNTAAQS
                     ITYQLTYTPANTDGTQNGKQTTISVAGGETKALNDIVKDWYGSGSLGEAGIGSLEIRP
                     LNYAGKDPVSITLATVASSRTYSVTSLGTFGQFIPALQLANFLSKSDTSKISLQQVAQ
                     SNASGGFRTNLGFVEGAGQPVDFVATLFDDAGNKLAERSYSLKPYESQQIKLDTFFNV
                     NGVVLPNVADARVEVRVTSDTGRISSYASVLDNTTTDPLLVFPTDASKIATSRFVLPG
                     VAEFDNGFSNFHTDMRVFNGGASTANVTLQYYAQGASTPAATTQVSIAPGEVKALDNV
                     LVKQFNLTTGNGSVVATTPSDSSLVVTARTFSRDKATGGTFGQFIPGVTTADSVGNGD
                     RALQVLQLEESPAFRTNLGLVEVTGADATVELLAYSPDSKVASRLVVPLPAGQYIQKG
                     GIFKSMGFNNVYNGRITARVISGTGRIAAYGSVVDNRTTDPTYVPSQ"
     gene            2439..2669
                     /locus_tag="VNN25_09060"
     CDS             2439..2669
                     /locus_tag="VNN25_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91717.1"
                     /translation="MLTVGLALFLYTAAANVQPIALAHQPSMVAHAGGVTSPDHAPPH
                     PLSPRVPLVEQAPKTFLGAVLQAAKTALTHAR"
     gene            complement(3089..3469)
                     /locus_tag="VNN25_09065"
     CDS             complement(3089..3469)
                     /locus_tag="VNN25_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HXH91718.1"
                     /translation="MAHTSERLRRFARGMRREPTRAESRIWGWLRARRFSEWKFRRQH
                     PIGRHIVDFYSAELKLAIELDGSHHAAEWMIDHESERTRDLRNRGIEVLRIPNELLIR
                     DSEWVGEAIKTAIERRVADLRRLT"
BASE COUNT          760 a         1383 c         1080 g          688 t
ORIGIN      
        1 ccgggaactt cggcgtgccg atctccgtct ccgttgtcgc ctccggcgcg gtctcctacc
       61 agtggtatcg cgcagcgtcg ggcgatccat ccaaccccgt ttccggcggc accggcccgg
      121 tgctcaccgt caccccgcaa gccaccggct tcacggacta ctgggtgaag gtcaccaacg
      181 cttgcggcag cacaaacagc agcaccgtga cgatcacgat caactgtgcg ccgcggacga
      241 tgtcggtccg cacaccgccg gccgtgcttt caggatcgac gtacaccgtc tcctggaact
      301 tcgatccgca actcgacgtc cgcaacgaac tgcaggaagc gaccaaggcc gatttcagcg
      361 atgccgtgaa cttcgaagtg accggcgtgg gctcgcgcac attcacgcat accgtgacgg
      421 ccgatacgcg ttactactac cgcgtcgcaa acgctccgct ctgtggaggc ctcggctcgt
      481 atgggcaggc cggcagcact ctcgtcaccg cgccgcaagc tgcgacgcag gccaatttcg
      541 acttcgccca ggcgccgtgc accggtccga actgcgccgt ccgtcagtcg ctcttcatcc
      601 caggtttcgt aacggccggc aagaccgcgc tggctctggg tgacacctac agcgtcagct
      661 ccgatgaatc gttcgtcacc gtctcgccgt cgagcggtgc tctgcctgcc gagggggtga
      721 cggtcaccgt tacgatcgac acgtcacagc tcgacttcgg cgccacgcag gccagcgtca
      781 ccgtcatccg cacgcccgcc ccagggaaaa ccggcgcact cggcgatccg cccgctacca
      841 acaccgttcc tatctccgtc agcgtcgtcg cgccggtcac cccggtaccg aaagatccga
      901 acgccccctt caacgcgctg ctcatccctg ccatcgcgca cgccgacggc atcggcacgc
      961 gcttcgtcag cgatgttcgc ctgaccaaca ccgccgcgca gtcgatcacg tatcaactca
     1021 cgtacacgcc ggcgaatacc gacggcacgc agaacggcaa gcagacaacg atctcggttg
     1081 ccggcggcga gacgaaggcg ctcaacgaca tcgtcaaaga ctggtacggc tcgggatcgc
     1141 tcggcgaagc gggaatcgga tcgctggaga tccggccgct gaactacgcg ggcaaagatc
     1201 ccgtcagcat cacgctggcc accgtcgcct ccagccgcac ctacagcgtg accagcctcg
     1261 ggacgttcgg tcagttcatc ccggcgctgc aactggcgaa cttcctcagc aagagcgaca
     1321 cgtcgaagat ctcgttgcaa caggtcgcac agagcaatgc cagcggcgga ttccgcacca
     1381 acctcggctt cgtcgaaggc gcaggccagc cggtcgattt cgtggccact ctgttcgacg
     1441 atgccggcaa caagctcgcc gaacgctcct acagcctcaa accgtatgag tcacagcaga
     1501 tcaagctcga caccttcttc aacgtgaatg gcgtcgttct ccccaacgtc gccgacgccc
     1561 gcgtcgaagt gcgcgtcacc agcgacacgg gccgcatcag ttcgtacgct tccgtgctcg
     1621 ataacaccac gaccgatccg ctgctcgtct tcccgacgga cgcgtcgaag atcgcgacgt
     1681 cgcgcttcgt gctccccggc gtcgccgagt tcgacaacgg cttctcgaac ttccacaccg
     1741 acatgcgcgt cttcaacggc ggcgcgtcga cagcgaatgt gaccctccag tactacgcgc
     1801 agggagccag caccccggca gcgacgacgc aagtctcgat cgctcccgga gaagtgaaag
     1861 cgctcgacaa cgtgctcgtc aaacaattca acctgacgac gggcaacggt tcggtagtcg
     1921 ccacgacgcc gtccgactcc tcgctcgtcg tcaccgcccg cacgttcagc cgcgacaaag
     1981 ccaccggcgg cacgttcggc caattcatcc ccggcgtcac gaccgccgac tctgtcggca
     2041 acggcgaccg cgccctgcag gttctgcaac tcgaggagtc gcccgccttc cgcacgaacc
     2101 tcggcctcgt cgaagtgacc ggcgccgacg ccaccgtcga gctcctcgcc tactcgcccg
     2161 actcgaaggt agcctcgcga ttggtggtgc ctctccccgc gggccagtac attcaaaagg
     2221 gcggcatctt caagtcgatg ggattcaaca acgtctacaa cggccggatc acggcgcgcg
     2281 tcatcagcgg caccggccgc atcgccgctt acggttccgt cgtcgacaac cgcaccaccg
     2341 atccgacgta cgttccctca cagtagcgct tgcttcgtgc gcgcggctcc ggccgcgcgc
     2401 acgtgcttca tgagcgccac ggagctatca ttccattcat gctaaccgtc ggcctcgcac
     2461 ttttccttta caccgccgcg gccaatgtcc agccgatcgc gctggcacat caacccagca
     2521 tggtcgcgca cgccggcggc gtcacctcgc ccgatcacgc gccgccgcac cccctgtcac
     2581 cgcgcgtccc gctcgtggaa caagcgccca aaacgttcct gggcgcagtg ctccaggcag
     2641 caaagacagc gctcactcac gcacgctgac atcttctggc ttggcgaagt tgttaacgcg
     2701 acgcttccct tgagcgatgt ttatgacgcg cgaggatttc aggaaggaaa cgtaggcgag
     2761 tactggtccg ggaaaaacct acatcactga gttccaatcg agcgccttct cccccacgct
     2821 tttcagtggg ggagaaggtg gcgaagccgg atgagggggc gcttgcctgt cgcgacaaac
     2881 gtgtcaaaca gcgtcgtgac ttctcgccca taacctcaca cccgtcgact gcacgacacc
     2941 cctcatccgc cttcggcacc ttctcccctc gaaaagcacg aggggagaag gcgctcgatt
     3001 catgagcatc gcgtgactgc acgagcgtga tcgccaccct tcacgacaac ctctcccacg
     3061 agcgcgaagc cggatgaggg gtcgagcatc atgtgagccg acgcaggtcc gcgactctcc
     3121 gttcgatcgc agtcttgatc gcttcaccaa cccactccga atcgcggatc aacaactcgt
     3181 tcggaattcg aaggacttca atccctcggt tacgcaggtc gcgggttcgt tcggactcgt
     3241 gatcgatcat ccattcagca gcgtgatggc ttccatccaa ctcgatcgcg agcttcaatt
     3301 ctgcggagta gaaatcgaca atgtggcgcc cgattggatg ctgacggcga aacttccatt
     3361 cgctgaatcg gcgcgcgcgc agccatcccc agatccgcga ttccgcacgt gttggctccc
     3421 gccgcatgcc acgcgcgaag cgacgaagac gttcgctcgt atgtgccacg actccgagga
     3481 tacctcggtg aattgcattt gtgccgtcgg caaccagcaa cacctctaat gaaaatctac
     3541 atcactgagt tccaatcgag cgccttctcc cccacgcttt tcagtggggg agaaggtggc
     3601 gaagccggat gagggggcgt ttgcctgtcg cgacaaacgt tgccgaacag cgcgttgact
     3661 tctcgtccct aacttcacgt gcgccgtttg cacgacaccc ctcatccgcc ttcggcacct
     3721 tctcccctcg aaaagcacga ggggagaagg cgctcgattg atgagcaggc atgactactt
     3781 gagggcgcaa gcatgcgaac ccgcgaactg cgcagctatg gttttctctt cgcgacaacc
     3841 catcccacga gcgccttctc ccccacgctt ttcagtgggg gagaaggtgg cgaagccgga
     3901 tgagggggcg t
//
LOCUS       DATLVW010000293         4236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_346083, whole genome shotgun sequence.
ACCESSION   DATLVW010000293 DATLVW010000000
VERSION     DATLVW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346083"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1418)
                     /locus_tag="VNN25_09070"
     CDS             complement(87..1418)
                     /locus_tag="VNN25_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXH91719.1"
                     /translation="MSLPPGPRSVIPFRYLRVLQRDPIPFLTNVASTYGDAAQFYVGP
                     QQIFLFNHPDLIREVLVTQHRAFHKSRVLQRSKIIFGEGLLTSEEEFHRRQRRMAQPA
                     FHRERIGHYGEVMIDRAVKRAATWRDGEELDIHREMMGLTLSVVAKTLFDADVDEDAD
                     AIGKALTALVDLFPALMNPLANLLRKLPLPQTMRFRRSIEMLDRTMYAIIDERRKSGD
                     DRGDLLSMLLLAVDEEGDGGGMTNLQLRDEAMTLFLAGHETTANALAWTWYLLSQNPD
                     AAATMHREIDAVLGDRLPEPADYARLTYTEMVVAESMRMFPPAWAVSRLAMEEVVIGD
                     WLVPAGAVVIAAQAVTHRDPRWWPEPDRFDPLRFTPEGKAARPKMAYFPFGAGPRICI
                     GEGFAWMEAVLIVATLAQKWHFELVSRDVQAQASITLRPRGGVVMRLRARV"
     gene            1711..3561
                     /locus_tag="VNN25_09075"
     CDS             1711..3561
                     /locus_tag="VNN25_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HXH91720.1"
                     /translation="MNALPLILFFLAVTAIGYRYYSAFLAAKVMALDDSRVTPAHRFN
                     DGQNYVPTNKWVLFGHHFAAISGAGPLIGPVLAAQFGYLPGLLWLAFGVVLAGATHDF
                     VTLAASVRRNGKSLAEIARNEISPVAGVTAMIAILFIIVIALAGLGIAVVNALADSPW
                     GTFTIFLTIPIALVIGQYLHRSKAPYAIRNGSIAGALMVLGAVVAGRFIPGTALGTMF
                     TLTKPEITIAIAIYGFIASVLPVWMLLCPRDYLSSYMKLGTIALLVIGTIVVAPHIYA
                     PAVSQFAGGGGPIIPGKLFPFVFITIACGAISGFHALIGSGTTPKMIDKESDSRMIGY
                     GAMLCEGLVGCVALIAVCALHPGDYYAINVPPAVFAKLGLATVNLQHFSQVVGEALVG
                     RTGGAVSLAVGFAQIFSGIPLLGGLMSYWYHFAIMFEALFILTTIDTGTRVARFMAQE
                     FLGRAWKPFENTSWIPGTIITTGLVVFAWTWFILNGSISQIWPMFGIANQLLSAVALC
                     VGTTVILNSGKVKYAWTTMFPLAFVTTTTLYAGWRSIFDNFLGAKMMAKDPFTGYLDS
                     TLTAILMICVLVILTASVRAWLRAIRGENVTFVPATAGPDHRHMPGSGCC"
     gene            3574..3951
                     /locus_tag="VNN25_09080"
     CDS             3574..3951
                     /locus_tag="VNN25_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH91721.1"
                     /translation="MLGNQEIMAFIPTLDYDKARAFYEGVLGLKFVHNDGHAMVLDAN
                     GTTIRVAKVQKDWTPEQFTILGWKVNEIEKMAAALTERGVVFVRFGFFEQDALGIWTT
                     PSGDKVAWFKDPDGNTLSISQHV"
     gene            complement(4017..>4236)
                     /locus_tag="VNN25_09085"
     CDS             complement(4017..>4236)
                     /locus_tag="VNN25_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91722.1"
                     /translation="ALISAGLAMFLAVLYGRPSRSLWQALALAGLAGFCTAIAIHPVI
                     GYMSLTHLGPAIAGACVFILGMAMTKSV"
BASE COUNT          785 a         1433 c         1220 g          798 t
ORIGIN      
        1 gagagccacg acgcgctatt ttccctacct cccatatcgg cattgctctc ttcgcgtctt
       61 cgcgtcttcg cgtgaaacgg ttttcctcac acacgcgccc gcaaacgcat aaccacccca
      121 ccccgcggcc gcaacgtgat cgacgcctgc gcctgcacgt cgcgcgacac gagctcgaaa
      181 tgccacttct gcgcaagcgt cgccacgatc agcaccgcct ccatccacgc gaatccttcg
      241 ccgatgcaga ttcgcggacc ggcaccgaag gggaaatacg ccatcttcgg cctggccgcc
      301 ttcccctcgg gcgtgaaacg cagcggatcg aatcgatcag gttccggcca ccagcgcgga
      361 tcgcgatgcg tcaccgcctg cgccgcgatc accactgcgc cggcaggaac gagccagtcg
      421 ccgatcacca cctcttccat cgccagccgg ctcactgccc acgccggcgg aaacatccgc
      481 atcgattcgg ccacgaccat ctccgtgtag gtcaaccgcg cgtagtcggc gggctcggga
      541 agacggtcgc ccagaaccgc atcaatctcg cgatgcatcg tcgcggccgc atcgggattc
      601 tgcgacagca gataccacgt ccacgccagc gcgttcgccg tcgtctcgtg acctgccaga
      661 aaaagggtca tcgcttcatc gcgaagctgc agattcgtca tcccgccgcc atcgccttcc
      721 tcatccaccg ccagcaacag catcgacagc aggtcgccgc ggtcgtcgcc cgacttccgc
      781 cgctcgtcga tgatcgcgta catcgtccgg tcgagcatct cgatcgaccg ccggaatcgc
      841 atcgtctgcg gcaacggcaa cttccgcagc aggttcgcca acggattcat cagcgccgga
      901 aagagatcga cgagcgccgt cagcgccttc ccgatcgcgt ccgcatcctc gtcgacgtcg
      961 gcatcgaaga gcgtcttcgc cacaaccgaa agcgtgagac ccatcatctc gcgatggatg
     1021 tccagctcct cgccgtcacg ccacgtcgct gctcgtttca ccgcccgatc gatcatcacc
     1081 tcgccgtaat gcccgatccg ctcccgatga aacgccggct gcgccatccg cctctgccgc
     1141 cgatgaaact cctcctcgga cgtcagcaaa ccctcgccaa agatgatctt cgaccgctgc
     1201 aacacccggc tcttgtgaaa cgcccgatgc tgcgtcacga gcacctcgcg aatgagatca
     1261 ggatgattga agagaaagat ctgctgcggc ccgacataga actgcgcggc atcgccatac
     1321 gtactcgcga cattcgtgag aaacggaatc ggatcgcgct gcaaaacgcg cagataccgg
     1381 aaggggatga cgctgcgcgg gcctggcggg agcgacattg tcgtgtggaa gatagtgaaa
     1441 ccatcccacg cgaaggcgcg aagacgcgaa agggaggacc ggttttcttc gcgtattcgc
     1501 gccttcgcgt gagacgtctc tgagatcaaa ggagcaaatg aaccacagag acacagagat
     1561 cacagaggtt ctctctgtgt cctctgtgtc tctgtggtga tgctggctcc atggcagtaa
     1621 tgttacaacc acgcggcgcg gccgttccgt tcaacccgaa acattccaca tcccacattc
     1681 aacattcgcc tctaaaatca tccctacgcc atgaacgcgc tcccgctcat cctcttcttc
     1741 ctcgcggtca ccgcgatcgg ctatcgctac tactcggctt ttctcgccgc gaaggtcatg
     1801 gcgctcgatg actcgcgggt gacgccggcg catcggttca atgatgggca gaactacgtg
     1861 ccgaccaaca agtgggtgct cttcgggcat cactttgcgg ccatctccgg cgcggggccg
     1921 ttgatcgggc cggtgctggc ggcgcagttc gggtacttgc cggggctgct ctggctcgcc
     1981 ttcggcgtcg tgctcgccgg ggcgacgcac gacttcgtca ccctggctgc ttcggtgcgg
     2041 cggaacggca agtcgctcgc ggagattgcg cgcaacgaga tctcgccggt ggccggcgtc
     2101 acggccatga tcgccatcct cttcatcatc gtcatcgcgc tggccggact gggcatcgcc
     2161 gtcgtcaacg cgctggcgga ctcgccgtgg gggacgttca cgatcttcct gacgattccg
     2221 atcgcgctcg tgatcggcca gtacctgcat cgttcgaagg cgccctacgc gatccgcaac
     2281 ggctccatcg ccggcgcgct catggttctc ggcgccgtcg tggcggggcg cttcattccg
     2341 ggcaccgcgc tcgggacgat gttcacgctg accaagcccg agatcacgat cgccatcgca
     2401 atctacggat tcatcgcgtc agtccttccg gtgtggatgc tcctctgccc gcgcgactac
     2461 ctctcgtcgt acatgaagct cggcacgatc gcgctgctcg tcatcggcac gatcgtcgtt
     2521 gcgccgcata tttacgcgcc ggccgtgtcg cagttcgcgg gcgggggcgg cccgatcatc
     2581 ccgggcaagc tcttcccctt cgtcttcatc accatcgcct gcggtgcgat ctcaggattc
     2641 cacgcgctga tcgggtcggg aacgacgccg aagatgatcg acaaagagtc ggactcgcgg
     2701 atgatcggct acggcgcgat gctctgcgaa gggctggtcg gctgcgtggc gctcatcgcc
     2761 gtctgcgcgc tgcatcccgg cgactattac gccatcaacg tgccgcccgc ggtgttcgcg
     2821 aaactcggac tggccaccgt caacctgcaa catttctcgc aggtcgtcgg cgaggcgctc
     2881 gtgggacgta ccggcggcgc ggtctcactc gccgtcggct tcgcccagat tttctccggc
     2941 atcccccttc tcggcgggct gatgtcctac tggtatcact tcgccatcat gttcgaggca
     3001 ctcttcatcc tcaccacgat cgacaccggc acgcgcgtgg cgcgcttcat ggcgcaggag
     3061 tttctcggcc gcgcctggaa gccgttcgag aacacgtcgt ggataccggg cacgatcatc
     3121 accaccggcc tggtggtctt cgcctggacg tggttcatcc tcaacggatc catttcgcag
     3181 atctggccga tgttcgggat cgccaaccag cttctgtcgg ctgtcgcgct ttgcgtcggc
     3241 acgaccgtca tcctcaactc cggcaaagtg aagtacgcgt ggacgacgat gttcccgctg
     3301 gcgttcgtga cgacgaccac gctctacgcc ggctggcgct cgatcttcga caactttctt
     3361 ggcgccaaga tgatggccaa agatccattc acgggctatc tcgatagcac gctcaccgcg
     3421 atcctcatga tttgcgtcct cgtgatcctc accgcctcag tacgcgcgtg gctgcgcgcg
     3481 atccgcggcg agaatgtgac gttcgtcccc gccaccgccg gacccgatca ccggcacatg
     3541 cccggcagcg gctgctgctg aaacggaggg aacatgctcg gcaatcagga gatcatggcg
     3601 ttcatcccca ctctcgatta cgacaaggcg cgagcgttct acgaaggtgt cctcgggctg
     3661 aagttcgtgc acaacgacgg ccacgcgatg gtgctcgacg cgaatggcac cacgatccgc
     3721 gtggccaagg tgcagaagga ttggacgccg gagcagttca cgatcctcgg atggaaggtg
     3781 aacgagatcg agaagatggc cgcggcgctc accgaacgag gcgtggtctt cgtgcgcttc
     3841 ggtttcttcg aacaggacgc gctcggcatc tggactacgc cctcgggcga caaggtggcg
     3901 tggttcaaag atccggatgg caacacgctg tcgatctcgc agcatgtgtg aagcctatta
     3961 accacaaaga cacaaaggac acaaagatca tctctgtgcc ctttgtgtct ttgtggtcaa
     4021 acgcttttcg tcatcgccat gccgaggatg aacacgcaag caccagcaat cgcaggcccg
     4081 aggtgcgtca gactcatgta accgatcacg ggatggatgg ctatggcggt acagaagcca
     4141 gccagacccg cgagagccag cgcctgccac agactgcgcg aggggcggcc gtagagcacg
     4201 gccagaaaca tcgccaggcc ggccgagatg agcgcg
//
LOCUS       DATLVW010000294         7577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_464202, whole genome shotgun sequence.
ACCESSION   DATLVW010000294 DATLVW010000000
VERSION     DATLVW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464202"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..485
                     /locus_tag="VNN25_09090"
     CDS             213..485
                     /locus_tag="VNN25_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91723.1"
                     /translation="MKNELDEQIVLDENGNRTIDGKEIDHKDPLSYFSKDAPIMHGRF
                     GSYDVPRQRDRDIEAVVLADEERGIDDDVDPKFKAKIEKLLQFDAH"
     gene            complement(527..1159)
                     /locus_tag="VNN25_09095"
     CDS             complement(527..1159)
                     /locus_tag="VNN25_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91724.1"
                     /translation="MHFHEKVRERAHVRRIENAVDDEVRPAECDVNRRGALCVLQALA
                     QISDNAQLVGYSVILRVHRAPQTEVHREPHRFRRIIQVTLDMGRHGEDEIQNMSVRVL
                     RIRESQHRAEIMNLEASEEVRMTKSVNDLVEVDVRRFLLFRTGGCVMLDCRSKAGYFS
                     AELPERQNDVCTRDSGVGKSDLLSRRCLHAGPPLTSVFGFLDPPRWKGLL"
     gene            1214..2977
                     /locus_tag="VNN25_09100"
     CDS             1214..2977
                     /locus_tag="VNN25_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91725.1"
                     /translation="MTILGLRFPDDPLNFHPPALPDATPAVFVRGEPGYLQDAIERFS
                     SDERIDIRLIDGKSDFVAIAKEPISDFLTNNVALSLALRDHRIQKLETHLVFSEPQRP
                     DAERPERTIVGRTTNSLPGRCHCFNPAMEPVVSPNLPESLKQSIANLLFLCHSTLHDQ
                     MSCCDVFQAVLSAKRALTRLSRRVDELHAERQKLDREYLSEELMDPEKAGHIHQQCRM
                     NQGKIEAKLHELDVWHLLAERVLRQRTVATFDALLLQSDRSIVYRGSVQKFLFLADAL
                     MNDFAVRISKWKGVTPQFCAMGDSVPRILSLKGMGLVRIPPHHVFTLPFVIPDLWHEV
                     GVYWFYKYADVEKFKLGDRDLSDPELGELGDLYGDLVVVRHGFAGDFLRFLFSLTRGW
                     LDYYDREKLSPSVYAENYEDLLTRLVFAQVVADPKPISDSELDSIDRFISDARGIYAK
                     HVIKASHKTIREYFPEVHELNPRLARITGIVVTKLKLYARTLQEVFNETISADVPSRS
                     DRKSYADMVEGSNGRLLRFNDGHDMNALMGELYWQFQRHRIARITALKTGKSIPAPFQ
                     RMASVAKSAIIEYHRRHARRP"
     gene            complement(3089..4330)
                     /locus_tag="VNN25_09105"
     CDS             complement(3089..4330)
                     /locus_tag="VNN25_09105"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HXH91726.1"
                     /translation="MWFARNGAASPPGRIGFVSRCAVVAWMFVAGALVAPRIEAADLR
                     GIYVYSSDVSQISKGSANNVTAALGVPGVDGIVLVISWSSIEPAIGQYQWATLDQWTH
                     RAVTLGKKIDLTITAGIDTPSWLFQPAPSGAGASPLTFTISPHGGATGVCDTETIAAP
                     WDAAFLNRWDLLLAAVTAHLKADGTYSAVTLLRLTGVNRTTDEFRLPAETPQSTGLAC
                     VSDAVATWQQAGFRPSRLLQAWDQITNSFVKSFPDKSFSVAIIPQNAFPPIGEDGAVI
                     KGTPPDQNQALLANASHKLPARLVVQFNFLMPGETASSAVTQAAQTLGTFAAFQTNNY
                     FGSTGQGAGCSEPVTNPTPCTDATFLTMLETGIFPLGQSNSLRAQYIEIFPANANAFP
                     SAVFQAHDDLIAGQRRRAAHH"
     gene            4512..4739
                     /locus_tag="VNN25_09110"
     CDS             4512..4739
                     /locus_tag="VNN25_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91727.1"
                     /translation="MPQLHLYVPDEVAETAKARAKAAGKSLSSYLADLVVNEVAGDWP
                     EGFFENVAGGWKGEPLERPKQGRPERRDRLS"
     gene            4739..5146
                     /locus_tag="VNN25_09115"
     CDS             4739..5146
                     /locus_tag="VNN25_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH91728.1"
                     /translation="MYLLDTNACIQFLNGTSQSLIERVRAVPRSLVRLSSVVKAELLY
                     GARKSTRMADNLRLLESFFDTVASLPFDDRCAEEYGLLRNELDRAGTPIGANDLLIAA
                     TARSHRAILVTHNVREFSRVAELQTEDWESPAR"
     gene            5181..5780
                     /locus_tag="VNN25_09120"
     CDS             5181..5780
                     /locus_tag="VNN25_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91729.1"
                     /translation="MRTAFTTAIAILLLAAPLFAADEFTLYELLPPQSHQFAITYDVT
                     QAKEGAEWFFNPIRVGSIATKERVIARASGEDLKFEVVSGKEAKTSGLVAASTADDAQ
                     FIRVHLPGPVPKGGQTRIRIIKTYTDAKSYDVKDGLLVFERPLGIKRNVVLLPKGWEL
                     VGCGSPGIVSTDADGRVRVSFLNDRDDQLPVKLVARSLP"
     gene            5777..6409
                     /locus_tag="VNN25_09125"
     CDS             5777..6409
                     /locus_tag="VNN25_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91730.1"
                     /translation="MKRALILLLALIPASLLAQESVRRAEQDREISYWLLDPSTHQFR
                     ISHDFTVTRVGQKSVHSFVRKGSVVSPDAKMTDLDTGKDLVTHAVPGKDVNALGYYPT
                     PVDPDSIAVQGDLEHPVAEGRSTRIRVQETYTDPVGYTLEGADLVWKRTLGRPLNYVT
                     LPAGWMLTSVNTPAVISLDEEGRIKLRFTNTRNDELSIVIKARRRPAGKP"
     gene            6547..>7577
                     /locus_tag="VNN25_09130"
     CDS             6547..>7577
                     /locus_tag="VNN25_09130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH91731.1"
                     /translation="MIAAHVSLAKGTMLGPYEVDILLGVGGMGEVYRARDTRLGRTVA
                     IKVLLAGLTASSERRARFEREARVISALSDPHICALYDIGRDGDTEYLVMEYIEGETL
                     ADRIARGRLPLSQVLRFGTEIAQALQHAHRAGITHRDLKPGNVMLTASGVKLLDFGLA
                     KLAIPEPAPGGSNAPTLVNPITTEGMVVGTLIYMSPEQLEGQNIDHRTDIFALGCILY
                     EMATGQRPFSGSSRIAITSAILSADPAPIRSLEPATPPALERIVLTALEKNPDDRWQT
                     AHDVARQLRWLSESSSSPESLAATEAPRTKRGYFIAAATAALLGGLLVWGVTRFASTP
                     LPTPVAARL"
BASE COUNT         1540 a         2305 c         2231 g         1501 t
ORIGIN      
        1 gaattcaaag gtcggactcg cgtttagcga ccgcatgctc gctcaacctc cgatttcgtc
       61 attcgacggg cgtgggcggg tcggagtgat ccggaatggt ccgtgcagtt gctctgcggg
      121 atcgcttgac ggggcgggcc atatttcggt aacgtaagcc actccccaac acgattcgtg
      181 tgaaaaaaag aggcttcggg ccgaggtgat tcgtgaagaa tgagttggat gagcagattg
      241 tgttggacga gaacggaaat cggacgatcg acggcaaaga gatcgatcac aaggatcctc
      301 tcagctactt ctcaaaagat gcgccgatca tgcacggtcg tttcggcagt tacgacgttc
      361 cgcgtcagcg cgacagagac atagaagcgg tcgtgctagc cgatgaggaa cggggcattg
      421 acgacgacgt cgatccgaag ttcaaggcga agatcgaaaa gctccttcag ttcgacgccc
      481 actaagcgct gctgtgcagc gctgcttacg ctactgtagt ttcgtgctac agtaacccct
      541 tccacctagg tggatccaaa aatccaaaca ccgaagttag agggggaccg gcgtgcaggc
      601 aacgtcgact caagagatca ctcttgccga ctccggaatc gcgagtgcat acatcgttct
      661 gccgctcggg aagttcagca gagaagtagc ccgccttcga acggcaatcc aacatcacgc
      721 agccgcccgt gcgaaagaga agaaaccttc gaacatcaac ctcaacgagg tcatttacgc
      781 ttttggtcat gcggacctcc tcgctcgctt cgagattcat gatctccgcg cgatgttgcg
      841 attctcgtat ccgaaggact ctgacgctca tgttctgaat ttcatcctct ccgtgccgtc
      901 ccatatcgag ggtaacctgg atgatccggc gaaatcgatg cggttcacga tgcacctccg
      961 tctgcggcgc tcgatgtacg cggaggatga cgctgtatcc aacgagttgg gcgttatcgg
     1021 atatctgtgc aagcgcctgc aggacgcaca gtgcgccgcg acgattcacg tcgcactcgg
     1081 ctgggcggac ctcatcgtcg acggcgtttt cgatccgacg aacatgggca cgttcgcgaa
     1141 ctttctcgtg gaagtgcatg aaggatacgt tctcgacgcg agtggcggcc gccacgcggt
     1201 gtttcagcgg acgatgacga tactcggact tcgcttccct gacgacccgc tgaacttcca
     1261 tcctcctgca cttcctgacg cgacgcctgc cgtttttgtg cgaggagagc ccggctatct
     1321 acaggacgca atcgagcgat tctcgtcgga cgagcgaatc gacatccgcc tgatcgacgg
     1381 caagtctgat ttcgtcgcga tcgccaagga gccgatcagc gactttctca cgaacaacgt
     1441 ggcgctctct ttggcattgc gtgaccaccg gatacagaaa ctcgaaacac acctcgtatt
     1501 ctcggaaccg caacggcccg acgcggagcg ccccgaacga acgatcgttg ggaggacaac
     1561 taatagtctt ccgggccgat gtcattgctt taatccggcg atggagccgg tcgtctctcc
     1621 gaatctgccg gagtcgctga agcagtcgat tgccaatctt ctttttcttt gccattcgac
     1681 gctccacgat cagatgagct gctgcgatgt gttccaggcg gtcctgagcg cgaagcgcgc
     1741 cctaacccgc ctcagccggc gcgttgatga gctacacgcc gaacgccaga aattggatcg
     1801 cgaatacctg agtgaagagc tgatggaccc cgaaaaagcc ggtcatattc accaacagtg
     1861 caggatgaat cagggcaaga tcgaggcgaa actgcacgag cttgacgttt ggcacctcct
     1921 ggccgagcgc gtactgcgtc agcgcaccgt cgccacgttc gatgctcttc tgcttcaaag
     1981 cgaccgcagc atcgtctatc gcggcagcgt acagaagttc ctgtttcttg cggacgctct
     2041 catgaacgac ttcgcagtcc gaatcagcaa gtggaagggg gtgacgccac agttctgcgc
     2101 gatgggcgac tccgtgcctc gcatcctgag tctgaaggga atggggttgg ttcggatccc
     2161 tcctcatcac gtattcacac tgccctttgt tatcccggac ctctggcatg aggtgggtgt
     2221 gtattggttc tacaagtacg cagacgttga gaaatttaag ctgggggatc gtgacttgtc
     2281 ggaccccgag ctcggcgagc tcggcgacct ctacggagat cttgtcgtcg tacgtcacgg
     2341 tttcgcgggt gactttctgc gttttctgtt ttcgttgact cgcggctggc tcgactatta
     2401 cgatcgcgag aaactctcac caagcgtata cgccgagaat tacgaagatc tccttacgcg
     2461 gcttgtattc gcgcaagtcg ttgctgatcc caagcccatt tctgactcag agctcgattc
     2521 catagaccgt ttcatcagcg atgcgcgagg aatctatgcc aagcacgtca tcaaggcgtc
     2581 tcataagacc attcgagagt atttcccgga agtccatgag ctgaacccaa gactcgcgcg
     2641 cataacgggc atcgtggtca cgaagctgaa gctgtacgcg aggacactgc aagaggtgtt
     2701 caacgagacg atttcagctg atgtcccaag ccgctcagac cgcaagtcct acgccgacat
     2761 ggttgaagga tcgaacggta ggcttttgcg cttcaacgat gggcacgaca tgaacgcgct
     2821 catgggagag ctgtattggc agttccaaag acataggatc gcgcgcatca ctgccctaaa
     2881 aacgggcaag agtatcccgg cgcccttcca acggatggcc tcagtcgcaa aaagcgcgat
     2941 catcgagtac caccgtcgcc acgcccgacg gccttgatcc gacgcgagca actgacccct
     3001 cataagcgct aggtccccag ttcgactctg ggcaccgcca ccaaactccc ctcatcttta
     3061 tcaatcccgc gcgtcgccga tagcccactt aatggtgcgc cgccctacgc ctctgcccgg
     3121 cgatcagatc gtcatgcgcc tgaaacaccg cgcttggaaa tgcgttcgcg ttcgctggga
     3181 agatctcgat gtactgcgct cgcaacgaat tgctttggcc gagggggaag atgccagttt
     3241 ccagcatggt gaggaacgtt gcatcggtgc atggagttgg attcgtaacc ggctcggagc
     3301 agcctgctcc ctgccctgtc gatccgaaat agttgttcgt ctggaaggct gcgaaggtgc
     3361 cgagggtctg ggcggcctgc gtcacggcgc tcgaggccgt ttcgccgggc atcagaaaat
     3421 tgaactgaac aaccagcctc gcggggagct tgtggcttgc attcgcgagc agcgcttgat
     3481 tctggtctgg aggcgttcct ttgataactg ctccatcctc gccgatcggg gggaacgcgt
     3541 tctgcgggat gatggccacg ctaaacgatt tgtcgggaaa gctcttcacg aaggagttgg
     3601 tgatctgatc ccatgcttgg agcaggcgcg aaggacgaaa gccggcctgc tgccaggtcg
     3661 cgaccgcgtc gctcacgcac gccagacccg tgctctgcgg cgtttcggcc ggcaaccgaa
     3721 actcgtcggt ggtccgatta acgcctgtca ggcgaagaag cgtaacggcg ctgtacgttc
     3781 cgtcggcctt cagatgcgcg gtgacggcgg ccaacagcaa atcccaacga ttcagaaacg
     3841 ccgcatccca cggtgcggcg atcgtctccg tgtcgcagac gccggtcgcg ccgccgtgcg
     3901 gtgagatcgt gaaggtcagc gggctcgcgc cggcacccga aggcgcaggc tgaaagagcc
     3961 aggacggagt atcgatccca gcggtgatcg tcaggtcgat cttcttcccc aatgtgacgg
     4021 ctcgatgcgt ccactgatcg agtgtcgccc actggtattg cccaatcgca ggttcgatcg
     4081 agctccagct gatgacgagg acgatgccat cgactcccgg cacgccgagg gctgcagtga
     4141 cgttgttggc ggagcccttg gagatctgcg acacgtcgct gctgtagacg tagatcccgc
     4201 gaaggtcagc ggcctcgatc cgcggcgcaa ccagcgcgcc cgccacgaac atccatgcga
     4261 cgaccgcgca gcgcgaaacg aaacctattc tgcccggcgg cgaagcagcg ccgttccttg
     4321 caaaccacac tgttcctcct caccgggtgc ggaaacattg cgcagggatg ctgggattgt
     4381 caaggaggaa cccgagcgca agacgtaacg gttgccgcga aacggtgaga gctttcggct
     4441 cacgcctccc acgcgtccct tgcgttccga aacagtatcc tgctatatgt gcatacaacg
     4501 aaggatgact catgccccaa ctgcacctct acgttcctga cgaagtcgcg gagacggcga
     4561 aggccagagc gaaggccgca gggaaatcgc tctcctccta tctggccgat ctcgtggtca
     4621 acgaagttgc aggcgactgg ccggaagggt tcttcgagaa tgttgccggc ggctggaaag
     4681 gcgaaccgct ggagcgtcca aagcaggggc gtcctgagcg ccgtgaccgg ctgtcatgat
     4741 gtatctgctt gatacgaacg cttgtattca attcctcaac ggaacgtcgc aatccctgat
     4801 cgaacgcgta cgagccgttc cccgctcgct ggtgcgtctt tcctcggtgg tcaaagccga
     4861 gcttctgtat ggcgcgagga aaagcactcg gatggccgac aacctgcgct tgctggagag
     4921 tttcttcgac acggtcgcca gccttccgtt cgacgaccgt tgcgccgagg agtatgggct
     4981 attgcgaaat gagctcgatc gcgccggaac gccgatcgga gcgaacgacc tcctgatcgc
     5041 agcgacggca cgctcccatc gcgccatcct cgtcactcac aacgttcgcg aattttcgcg
     5101 cgtcgccgaa ttgcagacag aagactggga gtcgccggct cgctagtcgc caggtcgtat
     5161 gtgttgatat cgtgattcgc atgcgaaccg cattcacgac tgcaatcgct attctcctgc
     5221 ttgccgctcc cctcttcgcc gccgacgagt tcacgttgta cgagctcctg ccgccccaga
     5281 gccaccagtt cgcgatcacc tacgacgtga cgcaggcgaa ggaaggggcg gagtggttct
     5341 tcaatcccat ccgcgtcgga tcgatcgcca cgaaagagcg tgtgattgcg cgggcttccg
     5401 gcgaggatct gaaattcgaa gtcgtcagcg gcaaggaagc gaagacgagc gggctggtgg
     5461 ctgcgagcac ggcggacgac gcccagttca ttcgcgttca cctgccgggg ccagtcccga
     5521 aaggtgggca gacgcgcatc cgcatcatca agacgtacac cgacgccaag tcgtacgacg
     5581 tgaaagacgg gctgctcgtg tttgagcggc cactcggcat caagcgcaat gtcgtgctgc
     5641 tgccgaaagg atgggagttg gtcggctgcg gctcgccggg catcgtttcc actgatgccg
     5701 atggccgcgt acgcgtcagt ttcctcaacg atcgcgatga tcaattgccg gtgaagctcg
     5761 tcgccaggag cttgccatga aacgcgcgtt gattcttctt ctcgcgctga ttcccgcgtc
     5821 gctcctcgcc caggaatcgg ttcgtcgcgc cgagcaggat cgggagatca gctactggct
     5881 gctcgatccg tcgacgcatc aatttcgaat ctcgcatgac ttcacggtca cgcgcgttgg
     5941 acagaagtcg gtgcacagct tcgtgcgcaa gggcagcgtg gtctcgccgg acgcgaagat
     6001 gaccgatctc gatactggca aagacctcgt cacgcacgcc gttcccggca aggacgtcaa
     6061 cgcgttgggc tactacccga cgccggtcga tcccgattcc attgcggtgc agggcgatct
     6121 cgaacatccg gtggccgaag gacggtcgac gcggatacgc gtgcaggaga cgtacaccga
     6181 tccggtcggc tacactctcg aaggcgccga tctcgtctgg aagcgcaccc tcgggcgccc
     6241 cttgaattac gtcacactgc ctgccggctg gatgctgacc tcggtcaaca cacccgccgt
     6301 gatctcactc gacgaagaag gccggatcaa gctgcgcttc accaacactc gcaacgacga
     6361 gttgtcgatc gtgatcaagg ctcggcgcag accggccgga aaaccctgaa ccacagagac
     6421 acagaggtcg cagaggtcgc agaggtcgaa acgaaactct gtgacctctg tgtctctgcg
     6481 gtgcgacggt tgtgatcagc aacgagcgtc ctttagtaat cgcagagcgc aaccaattcc
     6541 tccgctatga tcgccgcaca cgtgagtctg gcgaagggaa caatgcttgg cccctacgag
     6601 gtggacattc tcctcggggt cggagggatg ggcgaggtgt atcgcgcccg cgacacgcgc
     6661 ctcggccgca ccgtcgccat caaggttctc ctcgccggcc tcaccgccag cagcgagcgg
     6721 cgtgcacgat tcgagcgtga ggcgcgcgtc atctccgcgc tctccgatcc gcatatctgc
     6781 gcgctttacg acatcggccg tgacggcgat accgaatacc tggtgatgga gtacatcgaa
     6841 ggggaaacgc tggccgaccg aattgcgcgc gggcgccttc cgctcagtca ggttctgcga
     6901 ttcggcaccg agatcgcgca ggcgctgcag catgcgcatc gtgccggcat cacgcatcgc
     6961 gatctcaagc ccggcaacgt catgctcacc gcgtcgggcg tcaagctgct cgacttcgga
     7021 ttggccaagc tggcgattcc cgaaccagcg cccggcggct ccaacgcccc gacgttggtc
     7081 aatccgatca ctactgaagg gatggtcgtc ggcacgctca tctacatgtc gccggagcag
     7141 ctcgaagggc agaacatcga tcaccgcacc gacatcttcg cgctgggctg catcctgtac
     7201 gagatggcca ccgggcagcg gccgtttagc ggcagctctc gcatcgcgat caccagcgcg
     7261 atcctctccg ccgatcccgc tccgatccgt tcgctcgaac cggccacgcc tcctgctctc
     7321 gaacggatcg ttctcaccgc gctcgaaaag aatcctgacg atcgatggca gacagcgcac
     7381 gacgtcgcgc ggcagttgcg atggctgtcg gagtcctcat cgtcgcctga gtcgcttgcc
     7441 gcgaccgagg cgccgcgaac gaaacgcggt tacttcatcg cagcggcgac tgcagcattg
     7501 ctaggcgggc ttctggtgtg gggcgtcacg cggttcgcat ccactccatt gcccacgccg
     7561 gtcgccgcac gcctcga
//
LOCUS       DATLVW010000295         3147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_422575, whole genome shotgun sequence.
ACCESSION   DATLVW010000295 DATLVW010000000
VERSION     DATLVW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422575"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VNN25_09135"
     CDS             complement(<1..401)
                     /locus_tag="VNN25_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91732.1"
                     /translation="MRQLLVVGSLVLLLTATTTFAQNAESFRLSAFLVNPADTPGLYP
                     GSPRAYGGAIEYFAKRNLSWQMAVSSEQTLITTFTAAGLPYTSFERIHPVDGTARFHF
                     GSSLVRPFLGAGVRYIRVAGHDNWRPELAGG"
     gene            complement(640..1221)
                     /locus_tag="VNN25_09140"
     CDS             complement(640..1221)
                     /locus_tag="VNN25_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HXH91733.1"
                     /translation="MRNARMVIVSLLFVVAPLYAQQPNRPNAVTLFVSDVGFGWSGSS
                     GVEYGADYGLALSHMFNDRFSGELAVTSQRIGHSVTTYSINGLPTTIASSDRVYPIDA
                     SVSYHFFTNSRWKPYLGAGARYSSDSVRYTSPLGDYHFSRHSTDAEITGGITFQFNPR
                     LGLRFDAKQVIGNPRDIGADAKLQASVGLSLRF"
     gene            complement(1328..1954)
                     /locus_tag="VNN25_09145"
     CDS             complement(1328..1954)
                     /locus_tag="VNN25_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91734.1"
                     /translation="MIFALLLAINFVAPTLSQPGVWPGDEVAVRNGEKVLVLVEKSRL
                     QCELEEQHVRVSTVKDSPDLPGNVVGKKVSFENDSEVVQVIARVPGLIPGPVETVEVQ
                     AEENAKHENPITFTFHGVEWRIEVLGNDLVITDGTQKQVLARRLDKETTVAWDLYWAG
                     DMDHDGKLDLLYVIHGENYTRDQLALSSAAKEGELVRVVAGEKHFFGC"
     gene            complement(1951..3129)
                     /locus_tag="VNN25_09150"
     CDS             complement(1951..3129)
                     /locus_tag="VNN25_09150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXH91735.1"
                     /translation="MKDIVILGGARTPIGSFQGTLSSLAAPKLGSIAIKGALERAGVT
                     ADQIEQVIMGNVLQAAIGQAPARQAGIGAGIPVGAGAVTVNKVCGSGLKAVMYAANDI
                     RCDEYEVAVAGGMESMSNAPYALPQARSGYRMGNGKLVDLMIHDGLWDPYGDKHMGNC
                     AELCAAKYAFTREAQDDFSRESYRRAQEAVKSGQFKKEIAVVELQSKKGTTIVSDDEE
                     PFAAPLDKMGSLKPAFQKEGGTVTAANASKINDGASALIISSADWAQKHGRKPIARII
                     AQATAAQEPEWFTTAPAKAIDIVLKRAGMSVGDIDLFEVNEAFAVVAMAAMKDASIPH
                     EKMNVFGGAVALGHPIGSSGSRILVTLLHAMESRNVKRGMVAICIGGGEAAAMIVERM
                     "
BASE COUNT          600 a         1025 c          919 g          603 t
ORIGIN      
        1 aatcctccgg caagctcggg gcgccagttg tcgtggcccg ccactcgtat gtaccgcacg
       61 ccggcgccga ggaagggccg caccaaggaa ctgccaaagt gaaagcgcgc agttccatca
      121 acgggatgaa tgcgttcgaa acttgtgtac ggcaaccctg ccgcggtgaa cgtggtgatc
      181 aaggtctgct cggacgagac ggccatctgc caggatagat tcctctttgc aaaatattcg
      241 atcgccccgc cataggctcg aggggacccc ggataaaggc caggcgtatc ggccggattg
      301 acgaggaacg cggaaaggcg gaacgattca gcgttctgtg cgaatgtggt tgtagctgtc
      361 agcagcagga caagggaacc aactacgaga agttgtctca tcgaaaaaat cctccataca
      421 gtgagacgtt gccagcgtgg cgaaggtttc tcggtcatcc gcaaatgcac gcttcctggc
      481 cccggaggag cgccgcggag ctttcacaac gtcgtggggc gaatgcgcct gggatcgaca
      541 cacgctggat caaacggctg cagcacagcc actcgccagc ggtacaccgg cgttcgcgat
      601 caacaacctg actgagctcg gcgacgcggt cgactgagct tagaagcgca agctcagtcc
      661 gaccgacgcc tgaagcttcg cgtctgcgcc aatatctcta gggttgccaa taacctgctt
      721 cgcgtcgaaa cggagtccca gcctaggatt gaactggaag gtgatgccgc cagtaatctc
      781 ggcatcggtc gagtgtctcg agaaatgata atcaccgagt gggctggtgt agcgaacgga
      841 atcgctcgag tagcgagcgc cagcacccag gtacggcttc caacggctgt tcgtgaagaa
      901 gtggtacgag acactggcgt cgatcggata gacgcgatcc gaggaggcta tagtcgtcgg
      961 aagaccattg atagaatatg tcgtgacgga gtggccgatg cgctgactcg tcaccgccag
     1021 ctctcctgag aaccggtcat tgaacatgtg gctgagcgcc aggccgtaat cggcaccgta
     1081 ctcgactccg ctcgatcccg accacccaaa gcccacgtcc gacacgaaca gagtcaccgc
     1141 attcggccga ttgggctgtt gcgcatacaa cggcgcgacg acgaacaaca acgaaacgat
     1201 caccatcctg gcgttacgca tgcttcctcc catggtttgt tacctccaca ccattcgacg
     1261 gcggctgtac gcaaaggtta caagccgtta cggcgccgcc atcaccgcgc ggcagatccg
     1321 cgcgacacta acaaccgaag aaatgctttt cgcccgccac gacgcgcacc agctctccct
     1381 cttttgccgc ggacgagagc gcgagctggt cgcgtgtgta gttctctcca tgaatcacgt
     1441 aaagcagatc gagctttccg tcgtggtcca tgtcaccggc ccagtagaga tcccacgcca
     1501 cggtcgtctc cttgtccagg cggcgggcaa gaacctgctt ctgcgttccg tccgtgatca
     1561 cgaggtcgtt gccgagcact tcgatccgcc actccactcc gtgaaacgtg aacgtgatcg
     1621 gattctcgtg tttcgcattc tcttccgctt gaacctcgac ggtctcgact gggccgggga
     1681 tcaggccagg aacgcgggcg atgacttgaa cgacttcgct gtcgttctcg aacgacactt
     1741 tctttcccac aacgttgccc ggaaggtccg ggctgtcctt cacggtggag acgcgcacgt
     1801 gctgctcctc gagctcgcac tgcaaccgcg acttctccac gagcacgaga accttctcgc
     1861 cgttgcgcac cgcaacctca tcgccgggcc acacgccagg ctgcgacagt gtgggcgcga
     1921 cgaagttgat cgcgagaagg agcgcgaaga tcacatccgc tccacgatca tcgccgccgc
     1981 ctcgccgccg ccgatgcaga ttgccaccat gccgcgcttc acgttccggc tctccatggc
     2041 gtgcagcagc gtgacgagaa tgcgcgagcc gctggaaccg atcggatggc cgagagcaac
     2101 cgcgccgccg aagacgttca tcttctcgtg cggaatgctt gcgtctttca tcgccgccat
     2161 cgccacgacc gcgaaggctt cgttcacttc gaagagatcg atatcgccga cgctcatgcc
     2221 ggcgcgtttg agcacgatgt cgatcgcctt tgccggcgcg gtcgtgaacc actccggctc
     2281 ctgcgccgcc gtcgcctggg cgatgatgcg cgcgatcggc ttgcggccgt gcttctgcgc
     2341 ccaatctgcg gacgaaatga tgagcgccga ggcgccgtcg ttgatcttcg acgcgttcgc
     2401 ggcggtcacc gtgccgccct ccttctggaa cgcgggcttg agcgatccca tcttgtcgag
     2461 cggcgccgcg aacggctcct cgtcgtcaga aacaatcgtc gtccccttct tcgattgcag
     2521 ctcgacgact gcgatctctt tcttgaactg gccgctcttc accgcctctt gcgcgcgccg
     2581 gtacgactcg cgggagaagt cgtcctgcgc ttcgcgcgtg aacgcgtact tggccgcgca
     2641 gagctccgcg cagtttccca tgtgcttgtc gccgtacggg tcccaaagcc cgtcgtgaat
     2701 catgaggtcg acgagcttgc cgttgcccat ccgataaccg ctgcgcgctt gcggcagcgc
     2761 gtacggcgca ttcgacatcg actccattcc gcccgcgacc gcgacttcgt actcatcgca
     2821 gcgaatatcg ttcgccgcat acatcacggc cttgagtcct gaaccgcaga ctttgttgac
     2881 ggtgaccgcg cccgcgccga ccgggatgcc cgcgccgatg cctgcctgcc gcgccggcgc
     2941 ctggccaatt gccgcctgca ggacgttgcc catgatcacc tgctcgatct ggtccgccgt
     3001 cacgcccgct ctctcgagcg cgcccttgat ggcgatcgac ccgagcttcg gcgcggcaag
     3061 cgacgagagt gttccctgaa acgatccgat cggcgtccgt gcgccgccga ggatgacgat
     3121 gtctttcaat tgaggctcct tgcggcg
//
LOCUS       DATLVW010000296         7906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_382237, whole genome shotgun sequence.
ACCESSION   DATLVW010000296 DATLVW010000000
VERSION     DATLVW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382237"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VNN25_09155"
     CDS             <1..339
                     /locus_tag="VNN25_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXH91736.1"
                     /translation="WPIEPAQPNYFGEVAERWHYLDGAGEIGIISSVTQPFCGSCTRA
                     RLSADGKLYLCLFAGTGHDLRGLLRGAADDETIAAAIRSRWHKRDDRYSEIRTEATPA
                     TKVEMSHIGG"
     gene            complement(623..1276)
                     /locus_tag="VNN25_09160"
     CDS             complement(623..1276)
                     /locus_tag="VNN25_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXH91737.1"
                     /translation="MSRRFDRFVDDLSRVELSSRACNQFARTGAEADRNAIRRRNLRL
                     YLEELDAIGPRLLLVGEAVSHRGGRLTGIAFVSESVMLAGVELANGARVLGEDRGYRK
                     ADLSTPRSTEASATMVWGTIRNIDPLPLLWNAFPFHPFVAGNPFSNRIPTPDELRIGE
                     RFVRRLIDLFGISRIAAIGNQASITLTRLGIEHNKVRHPSQGGKTKFVAGMEAISRS"
     gene            1315..1776
                     /locus_tag="VNN25_09165"
     CDS             1315..1776
                     /locus_tag="VNN25_09165"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXH91738.1"
                     /translation="MPLVTLTTFRTSQDAHLAKAVLEDEGVPAFLGDENVSSIAPYVV
                     PNGVRLQVAADDFDRAEGILNRIAGMDVAEKSTASAERAIRHPEQCPRCGSPDIRRTR
                     RLLGFSIAAALLFAVGVAVDQLVMTFYLAMAICIFFLVASPFRCANCGHRW"
     gene            1780..2478
                     /locus_tag="VNN25_09170"
     CDS             1780..2478
                     /locus_tag="VNN25_09170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXH91739.1"
                     /translation="MIRAFVPDSVNALGYLLLLFVGVLLPALIFRSARKLGSRPLPIT
                     RSRFFRQTIIFQLVLALLCAWAAWTHGILIMPLPERPLSSFGAAVLLYLVMLATLKLR
                     WKSRSSAAKQRLYDILPRTSHEFVEYVLLCIVAGMAEEFIYRGVLTGLLQRITGITLV
                     TVITLSVSFAVAHSMQGLRSVAGIFVIALACHTLVWLAQSLIPMMAVHFAYDLTAGVL
                     IPRWVEGGEAEIRD"
     gene            complement(2561..3703)
                     /locus_tag="VNN25_09175"
     CDS             complement(2561..3703)
                     /locus_tag="VNN25_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXH91740.1"
                     /translation="MTRILSILLLLLATSAEAQDVLLQQLASGLTQPVALTHAGDTRL
                     FITQQIGTIVIYDGTGIRATPFLDIRSLVLDSGERGLLSVAFDPHYSENGFFFVYYTN
                     RNGDNSIARYKVSAADPNVADVSSGTILLTIPHPNFANHNGGQLQFGPDGYLYIGTGD
                     GGSAGDPSNHAQDLTQLLGKILRIDVDHGLPYSVPASNPFFLRPGPRGEIWAYGLRNP
                     WRFSFDRETGDLWIGDVGQDKYEEVDFQPATSIGGENYGWRKMEGFHCYNPSSNCIDP
                     SFTMPVIEYPHELGACSISGGYRYRGTQIPAMRGAYLYGDYCSGTISSATQTGATWTP
                     KTLFTTSISISSFGEDVAGELYVLDVAKGIVYKIIPRNGPRRRAVR"
     gene            complement(3880..4209)
                     /locus_tag="VNN25_09180"
     CDS             complement(3880..4209)
                     /locus_tag="VNN25_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HXH91741.1"
                     /translation="MQSRGFADFWPEYVRAHSRAQTRALHAIGSVLAVVMLGLGFAVS
                     LWFLIGVPLVGYAFAWYAHFFVEHNRPATFEHPFYSLAADYRMLFLMMAGRMDAEVER
                     CCGEAAA"
     gene            4270..4500
                     /locus_tag="VNN25_09185"
     CDS             4270..4500
                     /locus_tag="VNN25_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91742.1"
                     /translation="MPTDFEQLLKDVFGDSLDRLTQFSSEQKQRLQSRLQDMARDAVK
                     DELTKLHTEVNDLRERVAALEKEKIDEAAETV"
     gene            complement(4801..6684)
                     /locus_tag="VNN25_09190"
     CDS             complement(4801..6684)
                     /locus_tag="VNN25_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91743.1"
                     /translation="MLTSVDEFRAAVARYYDTAHRPYREISISEVTVLTTADEELITL
                     PAEEVLRESHRLLILGGPGSGKTTLLDRHAYDLANSGSVAPIFAPLRRYVTTLRDLLE
                     AQVNRFANLTLPELAMPPGKDVAILLDGLDEVAPPLRNRAIQDIQEIDRQWSEAQIII
                     TTRSGYNVVDLAGKWRTAELLPLTDEQVRVALSRFSPAVAKEIFASRRLLAMARRPLF
                     LHTILAEAEQPGDVRQYVIDAATQYFVWRGHSKSLLPADVPVEAVGRGLELLAIEFVR
                     RRVTWLDVETASALLAMDPALETAGIVQAVFDTDVIENDGERLRFSHLSYTEAYAAKY
                     LRSSIRTTVSVLDLRAVLDSPSADRILADLFKLLSFEERSRFISQCTTATLASLMRFV
                     PEEMAALVHDERTRIQPTHVVVEALKAADRAEYRDKPRRDVLVIAVHGFNTRGPWKNT
                     LSVLLNLETDGTRFIYAPWDYGQFTVGILNPFARRRKLDDFQVFYNAVLDTYGVQRPE
                     VCAVAHSFGTYLVCNSMRLFDEIRFDRLILLRSVLPRRFPWTKLAHKCSSVLSELGGS
                     DGALIFAQFVPGLGASGRYGFKTPGEIVREHVNEYGEHSTVFGARYMRESWIPFLRGE
                     HPS"
     gene            complement(6860..>7906)
                     /locus_tag="VNN25_09195"
     CDS             complement(6860..>7906)
                     /locus_tag="VNN25_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXH91744.1"
                     /translation="GRYLGLELIYARPNRQDAFLTYREPSIGRFDIPVQVTAFQSDDS
                     RPHTHIVERGGFVEASRITGNQTRWSLRYEYRIAECKSGDLCARAQSVLLPGLDRNAT
                     NVAISSVTPAFFWDRRDDSLNPHRGFFTTESVEYAFPLVAANARFLKEFTQTSWYLPV
                     TERSTFAMSGRIGLIQDLGLGMQVDPDTGNLIPTSGVPLSERFTSGGDSSNRAYALDS
                     LGTACPTQASIDAGCRPTLIDIIDLNGHHTTAPLGGRALLILNAEYRFPIAGSFGGQL
                     FADAGNTFSDTNIRFGDLKYGVGTGLRYVSPVGPIRFDFGYKLKRQILFYNEKGQAVF
                     EKPFAYFITLGYAF"
BASE COUNT         1590 a         2370 c         2391 g         1555 t
ORIGIN      
        1 tggccgatcg agccggcaca accgaactat tttggcgaag tggccgagcg ctggcactac
       61 ctcgacggcg caggtgagat cggcatcatc tcgtcggtca cgcaaccgtt ctgcggaagc
      121 tgcacacgtg cgcggctctc cgccgacggc aaactctacc tctgcctctt cgccggcacc
      181 ggccacgatc ttcgcggcct cctgcgcggc gccgcggacg acgaaacgat cgccgccgcc
      241 atccgctcac gctggcacaa acgcgacgac cgctattccg agatccgtac cgaggcgact
      301 ccggccacga aggtggagat gtcgcacatc ggggggtgag ttggggttct ccattcaatc
      361 tcgaaccacg agattcgtgt catcctgaga ctgccggagc cgccctctga tctcgatcaa
      421 gtttgcgctg cgtggagcgg cggcgcagga cgtcgaagga tctcaaactg cgccgcacga
      481 gccacatgcc tggcattcgg agatccttcg ccgtccttcg gcgccacgtc atgcaccgcg
      541 aaggctcgag cagtgcggcg cctccggcag gctcaggatg acacaacagg tttgttcagc
      601 gcgttagcaa aaccacacgc ctttaactgc gcgaaatcgc ttccatcccc gccacgaact
      661 tcgtcttccc accttgcgac ggatgccgca ccttgttgtg ctcaatgccg agccgagtaa
      721 gcgttatcga cgcctgattc ccgatcgcgg cgatgcgcga gatgccgaac aggtcgatca
      781 ggcgtcgcac gaaacgctcg ccgatgcgca gctcatcagg cgtcggtatc cgattcgaga
      841 aaggattccc cgcgacgaac gggtgaaacg gaaacgcgtt ccaaagcagc ggtagcgggt
      901 cgatgttgcg gatcgttccc cacaccatcg ttgcactggc ctctgtcgac cggggcgtcg
      961 aaagatctgc tttgcgatag ccgcgatctt caccgagaac gcgcgctccg ttggcgagct
     1021 ccacgccggc cagcatgacg gactcgctca cgaacgcaat tccggtcagg cgtccaccgc
     1081 gatgcgatac cgcctcaccg acgagcagga ggcgtggccc gatggcgtcg agctcctcga
     1141 gatagagccg caggttgcgg cggcggatcg cgtttcgatc agcctcggcg ccggttcgcg
     1201 cgaactggtt gcatgcgcgc gagctcagct ccacgcgcga aagatcatcg acgaaacgat
     1261 cgaagcgccg cgacacggcg cgcatgatag cggcggccag tacaatccgc ggcgatgccg
     1321 ctcgtaacgc tgaccacatt ccgcacctcc caggacgccc atctcgccaa agccgtgctc
     1381 gaagacgaag gcgttcccgc cttcctcggc gacgagaacg tctcctcgat cgcgccctac
     1441 gtcgtgccga atggcgtgcg tctccaggtc gcggcggatg atttcgatcg cgccgagggg
     1501 attctgaatc gcattgcggg gatggatgtc gccgagaaaa gcacggccag cgccgagcgc
     1561 gccattcgtc atccggagca atgtccccgc tgcggctctc ccgacattcg ccgcacccgc
     1621 cgtctcctcg gcttctccat cgccgccgcg ctgctcttcg ctgtcggcgt cgccgtcgat
     1681 caactggtca tgacgttcta tctggccatg gccatctgca tcttttttct cgtggcctcg
     1741 ccgttccgct gcgcgaactg cggccatcga tggtgaatcg tgattcgcgc cttcgttcct
     1801 gacagcgtca acgcgctcgg ttacctgctg ctgctgttcg tcggcgtcct gctgcccgca
     1861 ctgatcttcc gatcggcgcg aaagctcgga tcgaggccac tgcctatcac gcgatctcga
     1921 ttctttcgcc agacgatcat ctttcagttg gtgctggcgc tgctgtgcgc atgggcggcg
     1981 tggactcacg gaatcctgat catgccgttg ccagagcgcc cgctgagctc attcggcgcg
     2041 gcagtgctcc tctatctcgt gatgctcgcc acattgaagc ttcgctggaa gagccgcagc
     2101 agcgccgcga agcagcggct ttacgacatc cttccgcgaa cgtcgcacga atttgtcgag
     2161 tacgttctgc tttgcatcgt cgctgggatg gcggaggagt tcatctatcg cggtgttctg
     2221 accggactgc tgcagcgaat aacaggcatt acgctcgtga ctgtcatcac gctttcggtg
     2281 agcttcgccg tcgcgcactc gatgcagggt ttgcgcagtg tggccgggat cttcgtgatc
     2341 gcgctggcct gtcacacgct cgtgtggctg gcgcagtcgc tgatcccgat gatggccgtt
     2401 catttcgcat acgacctgac ggcaggagtg ctgattccgc gatgggtgga ggggggtgag
     2461 gcagagatca gagactagag atcagcgatc agacgggttg acgcgatgat gccctgatct
     2521 cttgtctctt gtctcttgtc tcctgtctct gatctctatc tcaccgcacc gcccggcgcc
     2581 gcggcccgtt gcgtgggatg atcttgtaga caatgccctt tgctacatcc agcacgtaca
     2641 gctcgcctgc gacatcctca ccgaagctgc tgatgctgat cgaagtcgtg aagagcgtct
     2701 tcggagtcca ggtcgccccc gtttgcgtcg cgctcgagat cgtgccgctg cagtagtcgc
     2761 cataaagata cgcgccgcgc atcgccggga tttgcgtgcc gcggtatcga tagccgccgc
     2821 tgatcgagca ggctcccaac tcgtgcggat actcgatcac tggcatcgtg aacgaaggat
     2881 cgatgcagtt cgatgaaggg ttgtagcagt ggaagccctc cattttgcgc catccgtagt
     2941 tctcgccgcc gatgctcgtc gccggctgaa aatcgacctc ttcgtatttg tcctggccga
     3001 cgtcgccgat ccagaggtcg cccgtctcac gatcgaaact gaaacgccac ggattgcgaa
     3061 gtccgtaggc ccaaatctcg ccgcgcgggc cggggcgcag aaagaaggga ttcgaggccg
     3121 ggacgctgta cggcaagccg tgatcgacgt cgatgcgcag gatcttgccg aggagttgcg
     3181 tcagatcctg ggcgtgattg ctcggatcgc cggccgagcc tccgtcgccc gtgccgatgt
     3241 agagatagcc gtcagggccg aactgcagtt ggccgccgtt gtggttggcg aagttgggat
     3301 ggggaatggt cagcagaatc gtgcccgacg acacatcggc aacgtttgga tcggcggccg
     3361 agactttgta gcgggcgatg gagttgtcgc cgttcctgtt cgtgtagtag acgaagaaga
     3421 atccgttctc gctgtagtgc ggatcaaagg caacgctgag cagaccgcgc tcgccgctgt
     3481 cgagcacgag cgatcggatg tcgaggaagg gcgttgcacg aattccagtc ccgtcgtaga
     3541 tgacgatcgt cccgatctgc tgggtgatga atagacgcgt atcgccggcg tgggtcagcg
     3601 cgaccggctg agtgagtccg ctagcgagct gctgaaggag aacgtcttgc gcctcggcag
     3661 acgttgccag cagaaggaga aggatcgaga gaatgcgggt catgcgcggc tcgatgcaat
     3721 tggttggccg ggagtcggtt ttcgggtgtc gggtgtcgga gcagtcatgc cgcgttttca
     3781 gaaccaatcg tcgagcggaa agaacccgaa gcgcatcact agaatcgtgc acgctttatc
     3841 tccgaccccc gacccccgaa aaccgacccc cgcgaaaacc tacgccgccg cctcgccgca
     3901 acacctctcc acctcggcat ccatgcgccc ggccatcatc aggaaaagca tgcggtagtc
     3961 ggcagcgagc gagtagaacg gatgttcgaa cgtggcgggg cggttgtgct ccacgaaaaa
     4021 gtgcgcgtac caggcgaagg cgtaaccgac cagcggcact ccgatcagaa accagaggct
     4081 gacggcgaag ccgaggccga gcatcactac cgcaagcacc gaaccgatcg cgtgcaaggc
     4141 gcgcgtctgt gcccgggagt gggcgcgaac atattccggc cagaaatcgg cgaagccgcg
     4201 gctctgcatg gaaactggga gattctccca cgttcctcca tggcacttca attgctgtgc
     4261 tcccttgcca tgcccaccga cttcgaacaa ctgctcaaag acgtcttcgg tgattcgctc
     4321 gaccgcttga cgcaattctc atccgaacag aagcaacgcc tccagtcgcg tctccaggac
     4381 atggcccgcg acgccgtgaa agacgagctg acaaagctcc acaccgaggt caacgacctc
     4441 cgcgagcgcg tcgccgcgct ggaaaaagag aagatcgacg aggcggcgga aacggtttga
     4501 ttcgcggctg cggggtcgcg aggttacgcg gtgtgatagt tgccggttgc ttggttttca
     4561 cgttggtgtc tgcgtgctca agctgatttg ccctccggag ccgcgcagac ttgaagccaa
     4621 ctgaccacga tcgccaaatc catggctgaa gaccatgcgc tccggtgatg gaacggcgac
     4681 cttgctcgcc accttttgca tgagtccgta ccgttggcac cgcctcgaaa cgttagggac
     4741 gggctcggcg catcgtaggt ttgtctctgc tgtgaggttc gagttcacgg cgaactcgca
     4801 ttaggaagga tgttcgcctc tcaggaatgg aatccacgac tcccgcatat atcgggcacc
     4861 gaacactgtg ctgtgctctc catactcgtt cacatgctcg cggactatct ctcccggtgt
     4921 tttgaagccg tatcgcccag atgctccgag gccagggacg aattgagcga aaataagggc
     4981 tccgtccgag ccgccaagtt cgcttaaaac ggacgaacat ttgtgcgcaa gctttgtcca
     5041 cggaaatcgg cgaggcagta cacttcgtaa caggataagc cgatcaaacc gtatctcgtc
     5101 gaacaaccgc atactgttgc agactagata cgtgccgaaa ctatgcgcga cggcacagac
     5161 ctctggccgt tgaacgccgt acgtgtcgag cacggcgtta taaaatactt gaaagtcgtc
     5221 caactttcgt cgccgcgcga agggattgag gatgcctaca gtgaactggc cgtagtccca
     5281 cggcgcgtag atgaacctag tcccgtcagt ctcgagattt agaaggacag acaaggtatt
     5341 cttccatggt ccacgcgtat tgaaaccatg cacagcaatc actaagacgt cacgccgagg
     5401 tttatccctg tattcagcgc gatcggcggc cttcagtgct tcgacgacaa catgtgttgg
     5461 ctgtattcgt gttcgctcat cgtggaccag agcggccatc tcctccggca cgaagcgcat
     5521 caaactcgcc aaagttgcag tagtgcactg tgaaatgaat cgagatcgtt cctcgaaaga
     5581 gagcagctta aataagtcgg caagaatacg gtcagcagac ggagaatcaa gaactgctcg
     5641 tagatcgagg acggaaaccg tggtacgaat gctagaccgc aggtatttcg cggcatacgc
     5701 ctcggtgtac gacaagtgtg aaaacctaag gcgctctcca tcgttctcga tgacgtcagt
     5761 gtcaaagact gcctgcacaa ttcccgcagt ttccagcgcg ggatccatcg ccaacaacgc
     5821 cgatgccgtc tctacgtcga gccacgtgac tcgcctgcga acaaactcga tagcgagtag
     5881 ctccaagcca cggccgacgg cctccacggg tacatccgct ggtagtagtg atttcgagtg
     5941 accacgccac acaaagtact gcgttgccgc gtcgatgaca tactgacgaa cgtcgccggg
     6001 ctgttctgct tccgccaaaa tcgtgtgaag gaacagcggc cttcgcgcca tcgcaagcag
     6061 acgccgggaa gcgaaaatct ccttcgcgac cgctggggag aatcgagaga gagccacccg
     6121 aacctgctcg tcggtgagcg gcaggagctc tgctgtccgc cattttccag caagatcaac
     6181 tacgttgtag cccgaacgtg tcgtaattat gatttgcgct tccgaccatt gccggtcaat
     6241 ttcctggata tcctgaatcg cgcgattacg aaggggtgga gctacctcgt cgagaccgtc
     6301 gagcaagatc gcgacgtcct tcccgggagg catcgcaagc tccggaagag tgagattggc
     6361 aaagcggttg acctgcgcct ctagaagatc gcggagcgtt gttacgtatc ttcgcaaggg
     6421 agcgaagatc ggtgctaccg agcctgagtt tgcgaggtcg tacgcgtgtc gatctaaaag
     6481 cgtagttttc cctgaacctg ggccgccaag tattagaagt cgatggcttt cgcgaagaac
     6541 ttcctcggct ggcagcgtaa tcaactcttc gtcggcagtt gttagtacag tgacctcgct
     6601 gatcgagatc tcgcggtaag gcctatgagc ggtgtcgtag taacgggcta cggcagctcg
     6661 gaactcgtcg acggaagtaa gcatcgcttg acagtgtagc ggactgggcc cttccgaagc
     6721 acatcgttcg cgtccaacca cactccgagt ggacctgaag cctcagagaa cttggtgcga
     6781 tcggcaacga gcggatgggg ctaccagctt cgctgccctg cgacctcgca agcccgagaa
     6841 tcccgcgact ccccgaacct cagaacgcgt accccaacgt aatgaagtac gcgaacggct
     6901 tctcgaacac cgcttggcct ttctcgttgt agaagaggat ctggcgtttg agcttgtagc
     6961 cgaaatcgaa gcggattggg ccgactggcg atacgtagcg caatccggtg ccgacgccgt
     7021 atttcagatc gccgaagcgg atgttggtgt cggagaacgt attgccggcg tcggcgaaga
     7081 gctggccgcc gaaagagcct gcgatcggga agcggtactc cgcgttgaga atgaggagcg
     7141 cgcggccacc cagcggcgcg gtggtgtggt ggccatttag atcgatgata tcgatgagcg
     7201 tcgggcggca accggcgtcg atcgaggcct gcgtcggaca cgctgtgccg agggaatcga
     7261 gtgcgtaggc gcggttcgat gaatcgccac cggaagtgaa gcgctccgac agcggtacgc
     7321 cgctggtcgg aatcaggttc ccggtatcgg gatcgacctg catgcccaac ccgaggtcct
     7381 ggatcaggcc aatgcgtccc gacatcgcaa acgtgctgcg ttcggtgacc ggcagatacc
     7441 acgacgtctg cgtgaactct ttcaggaaac gcgcgttcgc agcgacgagc gggaacgcgt
     7501 actcgaccga ttccgtagtg aagaatccgc ggtgaggatt gagcgaatca tcgcgcctgt
     7561 cccagaaaaa tgccggcgtg acgctggaga tggcaacgtt ggtcgcgttg cggtcgagac
     7621 ccggaagaag gacgctctgc gcccgcgcgc aaaggtcacc cgatttgcat tccgcaatgc
     7681 ggtattcgta gcgcagcgac caacgcgtct gattgcccgt gattcgtgag gcctcgacga
     7741 acccgccgcg ctcgacgatg tgcgtatgcg gccgggaatc gtcgctctgg aatgcggtga
     7801 cctggaccgg aatatcgaat ctgccgatgg acggctcgcg gtacgtgagg aaggcgtcct
     7861 gccggtttgg gcgcgcgtaa atcagttcca gaccgaggta gcgccc
//
LOCUS       DATLVW010000297        10106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_422684, whole genome shotgun sequence.
ACCESSION   DATLVW010000297 DATLVW010000000
VERSION     DATLVW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422684"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VNN25_09200"
     CDS             <1..2020
                     /locus_tag="VNN25_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91745.1"
                     /translation="GQLLDRPSILIASGTTATLGYPDVIFVGGAFLVAYPTGTSVVVR
                     RFSTDGRPIDIQPVVISNSAMAAWLATNGSTVFLPTARNRFRLLAADGTPLGPEREVP
                     NAGFGSFSVASNGDRYLIAYPAGPDKPGQGAFVILNANGDSLLSKPIPLADSLFLGHV
                     TASANGSSFLISMATNGPVRCMFVDADGNASALRTLDTQSAGSIIATWSGTEYNLVWP
                     RTLSTATRVTGHDIVGARVDASGTPIDTTPVTIAPSQPARYGTAFASAWNGRDTIIIT
                     GDSDGDDTNWRTTAAIFKSLPQIDAEPSARRRAAIASSAAEQASGSIASNGTLSLVTW
                     RESSGFDQAVVRAAFIAADGQLGSPIDLGAANAQTTTATASNGMDFLVAYVDTQSHLV
                     ARRVTLEGVLDPAPIVITPYGVPTDAMAIGWSGQAYVVVTAGGYAVTLSGISWDGTVT
                     LSRQVVNTSVHVDSPAVSCAANACSVTWHLASPLCFFDPCVSTENDVFARTSADGTLV
                     LQASLTDSVPITSALSLAAADGRSVFVYSSDKSMFAQRITAGGVVLDTPALNGGVKIM
                     TSETSFALQPVAVVHSGLYFVEPDNDTSGRLYWTRIEPEPKPHVTSLINLHQSVSLPL
                     TMTASSRNAYLLYSIGEDDPQLMAPRLYLRTIDSPDPQPGSTRRRSTR"
     gene            complement(2026..2226)
                     /locus_tag="VNN25_09205"
     CDS             complement(2026..2226)
                     /locus_tag="VNN25_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91746.1"
                     /translation="MSCEDDVNATGNPVACDHCERPFPPQETLCSVCDAPNPWQRRDT
                     LHFLCRECGTTQTYFSHLPIAG"
     gene            complement(2329..3999)
                     /locus_tag="VNN25_09210"
     CDS             complement(2329..3999)
                     /locus_tag="VNN25_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXH91747.1"
                     /translation="MTIEAPEKPRDFIREIVEDDLAAGRTQRVITRFPPEPNGYLHIG
                     HAKSICLNFGIANEYNGQCNLRFDDTNPTKEEVEFTESIKRDVAWLGFSWGEKLLHAS
                     DYFAQLYAWGEELINAGKAYVDSASADEMRAMRGTLTEPGVDSPHRSRSIEENLDLFR
                     RMRAGEFPDGAQVLRAKIDMASPNINMRDPVLYRIRHKTHDRTGDTWPIYPMYDYAHP
                     LSDAIEGITHSLCTLEYEDHRPLYDWLIENVSVPSRPRQYEFARLNLNYTVMSKRKLL
                     MLVSENHVHGWDDPRMPTIAGLRRRGYTPGSIRNFCERVGVAKRENVIDAGLLEFSIR
                     EDLNRIAPRVMGVLDPLRVVIDNYPADDTEWFETPNHVDDPSLGTRKVPFAKTIFIER
                     EDFLEDPPKKFFRLAPGREVRLRSAYFITCSSVVKDDAGNIVELHATYDPATRGGDSP
                     DGRKPKATLHWVAADHAIDCEVRLYDRLFKSENPEESGNFLDDLNPQSLQVVQAAKLE
                     PSIRGAQPLSTFQFERLGYFCVDSDSTRDRLVFNRTVTLKDAWAKVAK"
     gene            4127..4804
                     /locus_tag="VNN25_09215"
     CDS             4127..4804
                     /locus_tag="VNN25_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91748.1"
                     /translation="MKSALRQIVVLSLFLATPAAFAQSLDDVVASVLKEYGGAVAWQK
                     VTTIRETGTVIPAMGSGNGATTRSWQRPDKLRIEIVYPTRTELRVVDGDHGTNNGKDV
                     TGPGLDAMKLQAARLALPLLLVEKRSSLHDGGMRDGFRVIEVPVSASLTVGMDIDPKS
                     WHIVRSTGKTGGMDFVVDYNDFRRVDGLLFAFGESGMAQGTPTAKTTIDAIVINGPAP
                     QPPPAAK"
     gene            4806..5513
                     /locus_tag="VNN25_09220"
     CDS             4806..5513
                     /locus_tag="VNN25_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91749.1"
                     /translation="MTRSAWLQRLELPAAILLMLASCRAEPRRVIPWAWERREDLRFV
                     RGEVAYLAQTITLRGATIEVRPRMQPLLIDKSVRAIPVIRIETSHPSLDETQRSAAVD
                     AITSLRSATVQIDFDATLSERRFYRALIADLHRTIPHVTITALASWCSDDRWMSGLPI
                     EDAIPMLFQMGGDDHLVRTRLGRGEDFPEPVCRASAGISLDEMPPRIPGRRRIYVFSS
                     RRWSEPDWRLATERANR"
     gene            5510..6289
                     /locus_tag="VNN25_09225"
     CDS             5510..6289
                     /locus_tag="VNN25_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91750.1"
                     /translation="MKATRCALLFTAFATLIAWPTDSCSFSEGPAFVFAKRPDAPISA
                     YVAGRLGILMPTFAHSHLVVAWRYLAGKPLSEIEQKAVRAYYRQRLGEPRVTEQDPNR
                     DGVGEWVAVRDSVIASTSRPSNYRTEEMFFSVVNCTDDAFLTAAATLRSRIAEFGIKH
                     PGVRSWVEAQNAVFSNCQSRSTPPGPAATSLPVTFQHDRSYQIAAAAFYAAEFDRAHD
                     LFLVISREKASPWRRIARLVAARALIRKGVMATSTASIAFR"
     gene            6516..7817
                     /locus_tag="VNN25_09230"
     CDS             6516..7817
                     /locus_tag="VNN25_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91751.1"
                     /translation="MDQTFSVWSKGEHAPLQDAKAFAHAVERWQSKHTLPWLVASIAN
                     ATKADAHVDDLLEAAAKVEPSSPAFPMAAYHRARLLLDRGETDSAKGDLDRLLSLPAE
                     ALGASARNLFCELRMPLAASIDEFVNDAARLPAGTDLEGLADPPYETLFDDDSAVVFN
                     QALPLASLIDAARAPKLPNSIRAQLIAAAFTRAIVLGRTEVALSLVPEMHAHFDVAIK
                     EPLNRFAAAAPSRRRIEALDLLLHAPGLSPFVASYQGRVSSWLPLTELIHGSDHANWW
                     CVGGLSHGEIIYPRYVARRAALHVPKFAQARAMRDAFAAEEKDLRISGASFVLRGAIT
                     WAKEYPDDARVPEALATAISGTRWGCPDPATSKLASSAFDLLRSPLRRHEVGASNEAL
                     VQRSRLAGQRQPRLRLDHRDPLRVHLRAGMQAVGRDKRRVG"
     gene            complement(7707..8366)
                     /locus_tag="VNN25_09235"
     CDS             complement(7707..8366)
                     /locus_tag="VNN25_09235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXH91752.1"
                     /translation="MISAVAATLVLSIFPANAPPPRFLALGDSYTIGESVAEADRWPN
                     QLVRALRAGKTIVADPEIIAKTGWTTDELWAAIDAAKPHGPYSLVTLLIGVNNQYRGR
                     DAEQYRKEFVALLHRAIGFAGGDAKRVIVVSIPDWGATPFAEGRDRAKIGAEIDHFNA
                     IAREEAVRTGAHFADVTPVSRHASTDPTLVAPDGLHPSAKMYAQWVAVIEPEARLALA
                     R"
     gene            8398..8853
                     /locus_tag="VNN25_09240"
     CDS             8398..8853
                     /locus_tag="VNN25_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH91753.1"
                     /translation="MKRALITAALGFLAFSAVAANFASDEQALRRLDREMAVATYMAD
                     AEWFRTHLSDDYVRITSTGELQTKVELIALMKKQGTYIEPYETSDVQIHAHGSTAILT
                     GRIVQRYGEAGERVVAELRYSDVWIKTEEGWFNISGQVSPVAIKRERLK"
     gene            8985..9422
                     /locus_tag="VNN25_09245"
     CDS             8985..9422
                     /locus_tag="VNN25_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin SocA
                     domain-containing protein"
                     /protein_id="HXH91754.1"
                     /translation="MANAHDIANYILKKQGRMTAMKLQKLVYYSQAWSLVWDDKPLFH
                     DRIEAWINGPVLPHLYAVHRGMFDLKEWPRGNPENLSALQRETVDAVLEFYGDKSTQW
                     LSDLTHAELPWRDARRGLAPTERGDSEITPAAMAEYYGSLSRV"
BASE COUNT         1921 a         3207 c         3037 g         1941 t
ORIGIN      
        1 cggccagcta ctcgaccggc ccagcatcct gatcgcgtcg ggcactactg ccacgctcgg
       61 ctaccccgac gtcattttcg tgggaggcgc atttctcgtg gcctacccca cgggcacgtc
      121 agtcgtggtg cggcgcttct caacggacgg acgaccgatc gatattcaac ccgtagtgat
      181 cagcaactcc gccatggccg cctggctggc gacgaacggg agtaccgttt tccttcccac
      241 ggcgcggaac cgcttccggc tgctcgctgc ggacggcact ccgttgggcc ccgaacgcga
      301 agttccgaac gccggcttcg gctcgttctc cgtggcctcc aatggcgatc gatatctgat
      361 cgcctatccc gccggcccgg acaagccggg acaaggcgcg ttcgtcatcc tcaatgcgaa
      421 cggtgattct ctcctttcaa agccaattcc tcttgcagat tcgcttttcc tcggacacgt
      481 cactgcgtcg gccaatggat cttcattcct catttccatg gcaacaaatg gcccggtgcg
      541 ctgcatgttc gtcgacgctg acggcaacgc cagcgcactg cgcacgctcg acactcaatc
      601 cgccggatcg atcatcgcca cctggtccgg caccgaatac aacctggtgt ggccacgcac
      661 gctctcgacc gcaaccaggg taacgggcca cgacatcgtc ggagcgcgcg tcgacgcatc
      721 gggcacgccg atcgacacca cgccggtcac gatcgctccc tcgcagcccg cccgctacgg
      781 taccgcgttc gcgagcgcat ggaacgggcg cgacacaatc atcatcaccg gcgacagtga
      841 cggcgacgat acgaattggc gcacgactgc agccattttc aagtcactgc cgcagatcga
      901 tgcggaacct tcggcccggc gtcgcgcggc catcgccagc tcggcggccg agcaggccag
      961 cggctccatt gcttcaaacg gaacgctgtc tctggtcacg tggcgggaga gctcgggatt
     1021 cgatcaggcg gtcgtccgcg ccgcattcat cgccgccgat ggacagctcg gctccccaat
     1081 cgacctcggc gccgcgaatg cgcaaacgac cacagccacg gcatcgaatg gcatggactt
     1141 tctcgtcgcc tacgtcgata cccaatctca cctcgtcgca cggcgcgtga ctctcgaggg
     1201 tgtgttggat cctgcgccca tcgtcatcac tccctacgga gtgcctaccg atgccatggc
     1261 aatcggctgg agcggtcagg catacgtcgt tgtgactgcg ggaggctatg cggtgacgct
     1321 ctccggtatc tcttgggatg gcacggtgac cctctctcga caggtggtta acactagcgt
     1381 tcacgtggat agcccggccg tttcgtgcgc cgcaaatgcg tgcagcgtca catggcacct
     1441 ggccagtcct ctttgtttct ttgatccgtg cgtctccacc gaaaacgacg tattcgcacg
     1501 cacgagcgcg gatggcaccc ttgtcctgca agcgtctctg actgattctg tccccatcac
     1561 gtcagcactt tcgctggcgg cagcggacgg cagatccgtc ttcgtctatt cgagcgacaa
     1621 gtcgatgttc gcgcaacgga ttacggctgg aggcgtcgta ctggacactc cggcgctcaa
     1681 tggcggcgtc aagatcatga ccagtgagac gtcctttgct cttcaacccg tggcggtcgt
     1741 tcacagcggt ctctattttg tcgagccgga caatgacacg agtggccgcc tgtactggac
     1801 acgcatcgaa cctgagccga aaccgcatgt gacctctctg atcaacctgc atcagagcgt
     1861 ctcgctcccc ttgacgatga ccgcgtcttc gcgcaatgcg taccttcttt acagcatcgg
     1921 cgaggatgat cctcagctca tggcgccgcg gctctacctg cgaacgatcg actcgcccga
     1981 ccctcagccc ggttccactc gacggcgttc gacgcgctga atcagtcaac ccgcgatggg
     2041 caggtgcgag aagtaggtct gcgtcgtgcc gcactcgcgg caaaggaagt gcagcgtgtc
     2101 gcggcgctgc catggattgg gggcgtcgca gaccgagcag agcgtctcct gtggcgggaa
     2161 tggccgttcg caatgatcgc acgccacagg gttgccggtg gcattcacgt catcttcgca
     2221 gctcacctca ctgcagagcg gacatcgcca gcagacagcc atgcgcaggt tccccttcac
     2281 aagatagacg tcttggacca gcgcaagaat aacagcgaaa ggcaaaacct acttcgctac
     2341 cttcgcccaa gcatccttga gtgtcaccgt gcggttgaag accagccggt cccgcgtcga
     2401 gtcggaatca acacagaaat agccgagccg ctcgaactgg aaggtggaaa gcggttgcgc
     2461 cccgcggatc gacggctcga gcttcgcggc ctgcaccacc tgaagcgatt gcggattcag
     2521 atcatcgagg aaattgccgc tctcctccgg gttttcggat ttgaacagcc ggtcgtacag
     2581 ccgcacttcg cagtcgatgg cgtgatcggc agccacccag tgcagcgtcg ccttcggctt
     2641 tctgccgtcg ggtgaatcgc cgccgcgcgt ggccggatcg tacgtcgcgt gcagctcgac
     2701 gatgttgccg gcatcgtcct tcacgacgct cgaacaggtg atgaagtagg ccgatcgcag
     2761 ccgcacttcg cggccgggag cgagcctgaa gaacttcttg ggcggatctt cgagaaagtc
     2821 ttcgcgctcg atgaagatcg tcttcgcgaa cggaaccttt ctcgttccca gcgacggatc
     2881 gtcgacgtgg ttcggcgtct cgaaccattc cgtatcgtct gccggatagt tgtcgatgac
     2941 gacgcgaagc gggtcgagaa cacccatcac ccgcggagca atgcgattca aatcctcgcg
     3001 aatggagaac tcgagcagac ctgcgtcgat gacgttctcc cgtttggcca cgccgacgcg
     3061 ctcgcagaag ttgcggatcg atcccggcgt gtagccgcgg cgccgcaagc cggcgatggt
     3121 cggcatgcgc ggatcgtccc agccgtgcac gtggttctcg ctgacgagca tgagcagctt
     3181 gcgcttcgac atgacggtgt agttgaggtt cagccgcgca aactcgtatt gccgcggccg
     3241 cgacggcacg gacacgtttt cgatcagcca gtcgtacagc gggcggtgat cttcgtactc
     3301 gagggtgcaa agcgagtgtg tgatgccctc gatggcatcg gagagcggat gtgcgtagtc
     3361 gtacatcgga tagatcggcc aggtgtcacc ggttcggtca tgggttttgt ggcggatccg
     3421 atagagcacc ggatcgcgca tgttgatgtt cggcgaggcc atgtcgatct tcgcgcgtag
     3481 cacttgcgcg ccgtcgggaa actccccggc tcgcatgcgg cgaaaaaggt cgaggttctc
     3541 ttcgatggaa cgtgatcgat gcggcgaatc gacgcccggc tccgtgagcg tgccgcgcat
     3601 ggcccgcatc tcatcggcgc tcgcgctgtc gacgtaggcc ttgcccgcgt tgatcagctc
     3661 ttccccccag gcgtaaagct gcgcgaagta atccgaggcg tgaaggagct tttcgcccca
     3721 cgaaaagccg agccacgcca catcgcgttt gatcgactcg gtgaactcca cctcttcctt
     3781 cgtcggattc gtgtcgtcga accggagatt gcactggccg ttgtactcgt tggcgatgcc
     3841 gaagttcagg cagatcgact tggcgtgacc gatgtggagg tagccgttcg gctcgggcgg
     3901 aaagcgggtg atgacgcgct gggtcctgcc ggcagcgagg tcgtcctcga cgatctcgcg
     3961 gatgaaatcg cgcggttttt cgggggcttc gatggtcatg gggcgcgacc gtatacatcg
     4021 gcgtgcagcg agtcaacgcc cggcacggcc gggagcgctg gttgccaaag gctactggca
     4081 ccgtcatcgt gagcggaaag cgtcgatgca tgccacaatt cggatcatga aatcagcgct
     4141 caggcagatc gtcgtcctct ctctctttct ggccactccg gccgcttttg cgcagtcgct
     4201 cgacgatgtc gttgcgagcg ttctgaagga gtacggcggc gccgtcgcat ggcagaaggt
     4261 gacgacgatc cgggagacag gcacggtcat accggcgatg ggcagcggga acggcgcgac
     4321 gacgcgctcc tggcagaggc cggacaagct gcgcatcgag atcgtctatc ccacgagaac
     4381 cgagctgcgc gtcgtcgacg gcgaccatgg gacgaacaat ggcaaagacg tcaccggccc
     4441 gggcctggat gcaatgaagt tgcaggccgc gcggctggcg ttgccgctgc tcctggtcga
     4501 gaaacgctct tcactgcacg acggcggcat gcgcgacggc ttccgcgtca tcgaggttcc
     4561 agtctcagcc tcgcttacgg tcgggatgga tatcgatccg aagtcatggc acatcgtccg
     4621 atcgaccggg aagacgggcg gcatggactt cgtcgtcgac tacaacgact tccgccgcgt
     4681 cgacggactg ctcttcgcat tcggcgaatc agggatggcg cagggaacgc cgaccgcgaa
     4741 aacgacgatc gacgccatcg tgatcaacgg ccctgcaccg cagcctccgc ctgccgcaaa
     4801 atgacatgac ccggagcgcg tggttgcagc ggctcgaact tccagccgcg attctcctca
     4861 tgctggcctc ttgccgcgcg gaaccgcgcc gcgtgattcc gtgggcatgg gagcggcgtg
     4921 aagatctccg atttgtccgc ggcgaagtcg catatctcgc tcaaacgatc actctgcgcg
     4981 gcgcgacgat cgaagtgcgt ccccgaatgc agccactgct catcgacaag tctgtacgcg
     5041 cgattcctgt gatccgcatc gagaccagcc atccgtcgct cgatgagacg caacgaagcg
     5101 cagccgtcga cgcaatcacg agccttcgca gcgctaccgt gcaaatcgac ttcgacgcga
     5161 cgttatcgga gcggcgtttc tatcgtgcgc tcatcgccga ccttcatcgc acgattcctc
     5221 acgtcaccat caccgctctg gcgtcgtggt gcagtgacga tcggtggatg agcggacttc
     5281 cgatcgagga cgccattccg atgctcttcc agatgggcgg cgacgatcat ctcgtacgta
     5341 cacgccttgg ccggggcgag gattttcccg agccggtgtg tcgagccagc gccggcattt
     5401 ctctcgatga gatgccaccg cgcattcccg gacgtcgccg aatctatgtt ttcagctcgc
     5461 gccgatggtc ggaaccggac tggcggctgg ccaccgagag ggcgaaccga tgaaagcgac
     5521 tcgctgcgcg ctgttgttta ccgccttcgc gacactgatt gcatggccca cggatagttg
     5581 cagcttcagt gaaggtcctg ccttcgtctt cgccaagcgg ccagatgcgc cgatttctgc
     5641 ttatgtcgct ggccgcctcg gcatcctcat gccgaccttt gcccactcgc atctcgttgt
     5701 ggcatggcgt tatctagcag gcaaaccgct atcggagatc gagcaaaagg cggtgcgcgc
     5761 gtactaccgt caacggctcg gagaaccgag ggttaccgaa caagatccta accgggatgg
     5821 cgttggcgaa tgggttgcgg tgcgcgactc cgtcatcgcc tcgaccagcc ggccgtccaa
     5881 ctatcggacc gaagaaatgt ttttcagcgt cgtgaactgc acggacgatg cgttcctgac
     5941 agcggcggcg acactccggt cgcgcatcgc ggaattcggc atcaaacatc ccggcgtacg
     6001 cagttgggtc gaagctcaga acgctgtttt ttccaactgc cagagccgaa gcacgccacc
     6061 cggaccggcg gcgacctcac tgccggttac gtttcagcac gatcgaagct accagattgc
     6121 cgctgcggcg ttttacgcag cggagttcga tcgcgcccac gacctctttc tcgtgatcag
     6181 ccgtgaaaag gcttcgccct ggcggcgcat cgctcggctc gtcgcggccc gtgcactcat
     6241 ccgaaaaggc gtgatggcaa cttcgacggc ttcgatcgcg ttccgttgaa gggggcggac
     6301 caggagctgc gtgcaatcct cgcagacgag gatatgcgcg aactgcacca cgcggcgtcg
     6361 gagctcgagg actacgttca acttcacctt gcgccggaag agcagcgagc gaaactcgcg
     6421 cgagcgatca tgggtgggca catcacgcag catgatctga ccgacttcgt atcgatcttc
     6481 gacaaatccc cgaatgcgtc cgacgagatg accgattgga tcaaaccttc tcggtctgga
     6541 gcaagggcga gcatgctccg ttacaggacg cgaaagcatt cgcgcatgcg gttgagcgat
     6601 ggcagtcgaa gcacacactg ccgtggctcg tcgcgtcgat cgcgaatgcg accaaagcgg
     6661 acgctcatgt cgacgacctc ctcgaggcgg cagcgaaggt cgagccctcc tcgcccgcgt
     6721 ttccgatggc cgcttatcac cgtgcccgtc ttcttctcga tcgaggcgag acagacagcg
     6781 ccaaaggaga tcttgaccgc ctcctttcgc tgccggccga ggcactcggt gcttcggcgc
     6841 gcaatctctt ttgcgagctg cgcatgccgc tggcagcctc gattgacgaa ttcgtgaatg
     6901 acgcggcgcg tctgccggcg ggaaccgacc tcgaaggcct ggctgatccg ccatacgaga
     6961 cgctcttcga tgatgattct gcagtcgttt tcaatcaagc tctgccactg gcgtcgctca
     7021 tcgacgcggc ccgggcgccg aaactgccga actcgatccg ggcgcaactc attgccgcgg
     7081 cgttcacgcg cgcgatcgtg ttgggccgta ccgaggttgc cttgtcgctt gttccggaaa
     7141 tgcatgctca cttcgacgtc gccatcaaag agccattgaa tcgtttcgcc gccgcggctc
     7201 cctcgcggcg tcgcatcgaa gcacttgatc tacttctaca cgcgccgggc ctctcgccat
     7261 ttgtcgcgtc ttatcaaggg cgagtgagct cctggcttcc attgacggag ctcattcacg
     7321 ggtccgacca cgcgaactgg tggtgcgtcg gtggcctttc gcatggcgag atcatttatc
     7381 cgcggtatgt cgccagacgc gctgcccttc acgtgccgaa gttcgcccag gcccgagcga
     7441 tgcgcgatgc gttcgcggcc gaggagaagg atctgcgcat cagcggcgcg tcgttcgtgc
     7501 tgcgaggagc gattacgtgg gcgaaggagt accccgacga cgcgcgcgtg cccgaagcgc
     7561 tcgcaacagc aatcagcgga acccgatggg ggtgccctga ccccgcgacc tcgaagcttg
     7621 catcgtctgc gttcgactta cttcgctccc cgctacggcg ccacgaagtg ggcgcgtcaa
     7681 acgaagcatt ggtacagcgg tcgcgactag cgggccagcg ccagccgcgc ctccggctcg
     7741 atcaccgcga cccattgcgc gtacatcttc gcgctgggat gcaggccgtc gggcgcgaca
     7801 agcgtcgggt cggttgatgc atggcgagag actggcgtga cgtccgcgaa atgcgcaccg
     7861 gtccgcacag cctcttcccg cgcgatggca ttgaagtgat cgatctccgc gccgatcttc
     7921 gctcggtccc gcccctcggc gaacggcgtg gcgccccaat cggggattga gacgacgatc
     7981 acgcgtttgg cgtctccacc agcgaaaccg atggcgcggt gcagcaacgc aacaaactct
     8041 tttcgatact gctccgcatc acggccgcgg tactggttgt tgacgccgat gagcaacgtc
     8101 acaagcgaat acggcccgtg cggcttggcg gcgtcaatcg cagcccacaa ctcgtccgtc
     8161 gtccatccgg ttttggcgat gatctccgga tcagcaacga tcgtctttcc tgcgcgaagc
     8221 gcgcgcacca gttgattcgg ccaacgatcc gcctccgcta cgctttcgcc gatggtgtag
     8281 gaatcgccga gggcgagaaa acggggcggt ggagcgttcg caggaaagat cgagagaacg
     8341 agagtggcgg caacagcgct aatcatccgc agagtgtacc cgtgccagaa tgaccccatg
     8401 aaacgcgccc tgatcacagc cgcccttggt ttcctcgcct tctccgccgt tgccgccaat
     8461 ttcgccagcg atgaacaggc actacgacgg ctcgaccgcg agatggccgt cgccacctac
     8521 atggcggacg cagagtggtt ccggacccat ctctctgacg actacgttcg gatcacgagc
     8581 accggcgagc tgcagaccaa agtagagctg atcgcgttga tgaagaagca gggaacctac
     8641 atcgagccct acgagacgag cgacgtccag atccacgcgc atggcagcac cgccatcctt
     8701 accggccgga tcgtccaacg ctacggagag gcgggagagc gtgtcgtcgc cgagctccgc
     8761 tacagcgacg tctggatcaa gaccgaggaa ggatggttca acatctccgg ccaggtctcg
     8821 ccggtcgcga tcaagcgtga gcggctgaaa taggacccgg tttacgctgg ctttcaacgc
     8881 gcctgcgatt ccacggcacg tagacattta cctcgcctcg gcatagccgt gggagaatcc
     8941 tggtttgaca agcaacgccg gactgcggcg agaggactca ggccatggcc aacgcacacg
     9001 acatcgcaaa ctacatcctg aagaagcaag gacggatgac cgcgatgaag ctgcaaaagc
     9061 tggtttacta ctcgcaggcc tggtcactcg tgtgggatga caaaccattg tttcatgacc
     9121 gaatcgaggc gtggatcaat ggtccggtgc tgccgcatct ctacgcggtt catcggggca
     9181 tgtttgatct caaggaatgg ccacgaggca atcccgagaa cctctccgca cttcagcgtg
     9241 agacggtcga cgccgtcctc gaattctacg gggacaagag cactcagtgg ctgagcgatc
     9301 tgactcacgc cgagcttccg tggcgagatg cgcggcgcgg tctcgctccc acggaacgcg
     9361 gagacagcga gatcactcct gcggccatgg cggagtacta cggcagcctc tctcgagtgt
     9421 agacagcttt ggcaaatcga gacaaacgtc ctaaagcggc ttcgcaaccg aacattgcga
     9481 aacggccgct tatcgggatg cagccaaggt tagggaacat tccgattgtc gctgcggacc
     9541 cgagtaccaa tcacctttca ccttcatggc gagtcgcgag actgaagctc gtcagtccgc
     9601 acgggtggcg cgatattgtc ccatcaaagc tgctagagat tcgcgcgaag ctggccaact
     9661 tcgaatcgat gacctggaac gacatcctcg tgaccgcgaa gaagcgcaac cactcggtgt
     9721 ccgtttcccg aatcagtaac gaagctcaga atcacctttc gtcgctcaat cttttcgacg
     9781 tagacgaagt gatctccttg cacctgaccg gaactgagag gatttggggc tttcgtcaag
     9841 gcgctgtgct tcaggttctc tggtgggatc cagagcatca cgtctgtccg tctctgctaa
     9901 agaacacatg acgttgcacg cgaaccccct gctaaccttt cgcctcgggc ggcgtagtta
     9961 gtagtcacag gaggcaacac catgacgcgc attcgcactg cagcgatcgc ggctgtgctc
    10021 gggctggcgt cgatctccgc cgcgcacgcc gcgatcgaca gccccatcca ccgcaacttc
    10081 aacgttcacg ccggcggaac gatcac
//
LOCUS       DATLVW010000298         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_139199, whole genome shotgun sequence.
ACCESSION   DATLVW010000298 DATLVW010000000
VERSION     DATLVW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139199"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="VNN25_09250"
     CDS             <1..1967
                     /locus_tag="VNN25_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH91755.1"
                     /translation="LAEADAGIMERYPELQHVYAGRRQLIVLDPWGGFVAELKAAFAD
                     QALFAGALNSLRQQSATFVRAAKAFQEGNVGPAQVLRAGALLDAGAVTAAKDAFQLGY
                     ATAQKSNDIETMQRAQIGLAAIDLPSQKAVRSLEEITEHPVTNEIAARAWMLLAHTYR
                     QNRATKQAIDAYQKSFALAPKPSMLAEAARRHLETLGSEPVSETLAGVAAGNVHLLYP
                     HKEMMVGSVTFGVATSADAARVDVYLDDARVAELTRQPFRTKVDLGMAPHVRVVRAVA
                     FDAQERKLGEERVTLNDTAGALGISIVAPAGDTVQSRTAVEATARIPAGTTLDGVDLY
                     WNETKIATLTAAPYRHELVLPSPSASGFIRVVAHASDGSTAEDVKMINAGGAAEQVRV
                     DAVQVYAIVQDRSGHYVDGLKASDFVVKEDGRAVTPQLQSGSGDPISIGIALDTSASM
                     QVVMNDVMDYANEFVVHSLGAADQTFVTAFDAEPHLVQPLTNNRKQLTNAIYDLHAAG
                     GTAIWDAVLYSLQQFRGVGGKRALVVFTDGINNSGTASLRDDLKYAREVGVPVYFVQI
                     YTGARGLDMSFDETGIKNLTESTGGAYFRFAGKKDLPRLFSQIRDDTRGQYLLTYVSP
                     SIAPRRNLRRINVEVPGKPVIVRATSGYYP"
BASE COUNT          444 a          758 c          754 g          421 t
ORIGIN      
        1 tgctagcaga ggcagacgcc ggcatcatgg agcgctatcc cgagctccag catgtatacg
       61 cggggagacg gcagttgatc gtgctcgacc cgtggggtgg ttttgttgcg gaactgaagg
      121 ccgcctttgc cgatcaggcg cttttcgcgg gcgcactgaa ctcgttgcgt cagcagagtg
      181 ccaccttcgt ccgcgccgcc aaggcctttc aggagggaaa cgtcgggccg gcgcaagtgc
      241 tccgtgcggg tgcgctactc gatgccgggg ccgtcaccgc ggcaaaggat gctttccagc
      301 tcggctacgc gacggcccaa aagagcaacg acatagaaac gatgcagagg gcgcagatcg
      361 gactggcggc catcgacctt ccctcgcaga aggccgtgcg gtcgctggaa gagatcacgg
      421 agcatccggt caccaacgag atcgccgccc gagcctggat gctgctcgcg cacacgtacc
      481 ggcagaaccg cgcaacgaag caagccatcg atgcgtacca gaaatcgttc gcgctggcac
      541 cgaagccgtc gatgctggcc gaggcggcac gccgccatct cgagacgctc ggctcggagc
      601 cggtgtcgga gacgcttgca ggcgtcgcgg ctggaaacgt tcatctgctc tatccgcaca
      661 aagaaatgat ggtgggaagc gtcaccttcg gagtcgccac ctccgccgac gccgcacggg
      721 tcgatgtgta cctcgatgac gcgcgggtgg ccgaactgac tcgtcaaccg tttcgcacca
      781 aagttgatct cgggatggcg cctcatgtcc gcgtcgttcg cgcggtcgca ttcgacgccc
      841 aggaacgcaa actcggcgag gagcgcgtga cgctcaatga caccgccggc gcgcttggca
      901 tcagcatcgt cgcacccgcc ggcgacaccg tgcaatcgcg cacggccgtc gaagcgaccg
      961 cacggattcc ggccggcacc acgctcgatg gcgtcgatct ctattggaac gagacgaaga
     1021 tcgccacgct gactgccgcg ccgtaccgtc atgagctcgt gctgccatcg ccgtccgcgt
     1081 ctggcttcat ccgcgtggtc gcgcatgcga gcgacggcag caccgccgag gacgtgaaga
     1141 tgatcaatgc cggcggcgcc gcggagcagg tacgcgtcga tgccgtgcag gtctacgcca
     1201 tcgtgcagga ccgcagcggt cattatgtcg acgggctgaa ggcctcggac ttcgtcgtca
     1261 aagaagacgg gcgcgcggtc acgccgcagc ttcagagcgg aagcggcgat ccgatctcga
     1321 tcggcatcgc cctcgatacg tcggccagca tgcaggtggt gatgaacgat gtgatggact
     1381 acgccaatga gttcgtggtg cattcgctgg gcgcggcgga tcagacgttc gtcaccgcct
     1441 tcgatgcaga gcctcatctg gtgcagccgc tgacgaacaa tcgcaagcag ctcacgaacg
     1501 cgatctacga tctgcatgcc gcgggcggca cagcgatctg ggatgcggtg ctctactcgc
     1561 tgcagcagtt ccgcggcgtg ggcggcaagc gagcgctggt cgttttcacg gacggcatca
     1621 acaactccgg caccgcctcg ctgcgagacg acctgaagta cgcgcgcgag gttggagtgc
     1681 ccgtctattt cgtccagatc tataccggag cgcgcggact ggacatgtcc ttcgacgaga
     1741 ccggcatcaa gaacctcacc gagtcgaccg gcggcgcgta ctttcggttc gctggcaaga
     1801 aggatctgcc gcgcctcttc tcgcagatcc gcgacgacac gcgcggacag tacctgctca
     1861 cctacgtctc cccgagcatc gctccgcgcc ggaaccttcg ccgcatcaac gtggaagtcc
     1921 caggcaaacc ggtgatcgtg cgcgccacca gcggctacta cccgtagaag ggggcagacc
     1981 cgaagtggaa gtgccagaaa acggaaatgg caggcgttca gctaatttcg gtcactttca
     2041 ctttgggtct gaccccttcc ccgtcaattc gggaagcttt gccgcctcaa cctggagttc
     2101 aggtgacgtt gggggtcaga cccgaagtgg aagtgctgga aaacggaaat ggacaagggt
     2161 tcggccaatt tcggtcactt tcactttggg tctgacccct cgcgccgact caggctctcg
     2221 ttcgctgccc gccgacgatc ttctcgaagc gttcgccgct gcgcgccagg gtccgcacgg
     2281 tttccgacag ctcctcggcc agctcgttgt tttggcgcgc gatgtcgcgc atcgagcttg
     2341 atgcgttcga tacctcttcg atctggccga gctgcga
//
LOCUS       DATLVW010000299         5461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_346410, whole genome shotgun sequence.
ACCESSION   DATLVW010000299 DATLVW010000000
VERSION     DATLVW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346410"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VNN25_09255"
     CDS             <1..864
                     /locus_tag="VNN25_09255"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH91756.1"
                     /translation="YLNTCGRITLNLTSTANPAAQGAPFTVTGTVVSPPAAAATGTLT
                     LKRGTTVLNSINLNGGNSIAATIDDLAPATYTFTAEYSGDSRFVPAIQSIQQVVIVPP
                     FGPPPHLNAISFGGPVQLSWYATLNTDHYEIERNNGAGWIAAGTSASAAFTDLGAPAS
                     SALLYRVRAKAANGTASEYSLTDLALTYAFTDGTIQAGVTAVKRVHVTELRDAANAVR
                     ALAGLGAMSWAEPTPDLVRVTHITELRTAIDQARAALHLPTLTHIDPTLTAGTSPIRA
                     AHFEDLRASMR"
     gene            878..1426
                     /locus_tag="VNN25_09260"
     CDS             878..1426
                     /locus_tag="VNN25_09260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HXH91757.1"
                     /translation="MGEPALILIDVQYAIDDPSWGNDRNNPDAEEKMAALLAHWRERR
                     WPIFHIRHASTDPNSTYRPGQPLFAFKKEVTPIDGEPVIEKSTNSAFIGTRLEEMLRS
                     RGVVQVVIAGVITNNSVEATARMSGNLGFQTFVASDATATFGRPDFAGRWRTAEEVHA
                     MSLANLAGEYATVLGTDEILNR"
     gene            complement(1540..4113)
                     /locus_tag="VNN25_09265"
     CDS             complement(1540..4113)
                     /locus_tag="VNN25_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH91758.1"
                     /translation="MLAAGTMLGPYKILTAIGAGGMGEVYRAQDTRLGRDVAVKVLTR
                     NLSGDVEALRRFEQEARAAGMLNHPNILAIYDIGNEGGQNYIVSELLEGESLRARIRH
                     GAIPPRKALDYAAQIARGLAAAHERNIVHRDLKPENLFITRDGHVKILDFGLAKLAGP
                     RVPNATPGDEHELTLPTTPTEPGRLMGTIGYMAPEQVRGGSGDNRSDIFAFGVILYEM
                     LAGVQAFRAESPIETLNSILKDDPPDFFELNIRVPGAIDRVVRHCLEKNPDERFQSAR
                     DLAFDLGSLSGLTSQAVSFLPMWRIRPRNLLKPLTIAAVILAAAAIAYLIGQRRGTQP
                     PPSYRRLTFRSGTIFNARFSPDGQTVFYGARWSGKPMSIFSVRADSPESRDLDMGADT
                     DILAVSRGGQLAISLHRHPIGYVRESGTLAQVPIAGGAPREILDDVEYADWSPDGRLA
                     VVRTVNGRCRLEFPIGTVLYDTVGWISHPRFNPAGDTIGLLDHPFFNDDRGSVVLVTL
                     ADKGHKALTKESQSIEGLAWTAAGNEIVFSSEDGNTSRSVRAVTLRGKERLIAASAGP
                     LWLHDVAPDGRILVTREIVRAGIVAIRGNDKPVDLSWFDYSVVRDLSADGKTIVFSES
                     GEAGGAIFGVYIRGIDGSPAIRLGDGTSEALSPDGEWVLSIPRNRNPAQIVMLPTGAG
                     QPRQITRDRINHRNARWMPDGKQVFFQGNEEGKPPKLWIQALDGSAPRAITPENVSGT
                     QVTPDGRYVLGRATDHKFYLYPIAGGAPLPVPALKTGDVPTHFSADGRSLFVATFGKI
                     PAMLYKIDLASGTRTLWREAMPADPSGLINVGPILVTPDGATTVYSYTRLLSDLYVIG
                     R"
     gene            4246..>5461
                     /locus_tag="VNN25_09270"
     CDS             4246..>5461
                     /locus_tag="VNN25_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXH91759.1"
                     /translation="MKPAQTIQLTPGKRVLFLTKDPELIRKQLRGELDLQMKDLRVED
                     LLDDINTDAMTPAWVCFSHKPEDIAEDAYAGLIVDGKRLFEHDALINGNFEVIVSGYR
                     KGVGSSRETATQCEVWSGIRIAVAASFAPIHAGNNINQGLLMADHAMLLRLQAGEGIP
                     IDEFCKGRDAITQLIIREGGLFPFAKAVARGEIQVPVPDTPKRPMTMGEKILARHVLV
                     GGGGAGVGSGSKTDSSVTGSQPPLPTPHTPYVQPGDAIVVEVDGGYTHEFTTAQVHYF
                     LEQEYGPDYAIKNPQKFAVFEDHLIYADDVARMRPFMGKIQTLRDLQRAFQQHTGCRD
                     FSAVDNISPGICHEVAREYIIDPGDFIQATDSHTCMGGVNNALAWGVGATEYADLVHQ
                     GFTQVEVPESIRF"
BASE COUNT         1013 a         1696 c         1781 g          971 t
ORIGIN      
        1 tatctgaaca cctgcggccg gatcacgctc aatctcacat cgaccgcgaa ccctgccgcg
       61 cagggcgcac cgttcacggt taccgggacc gtggtttctc cccccgccgc tgcggccacc
      121 ggaacgctga cgctcaagcg cggcacgacg gtgttgaaca gcatcaatct caacggaggg
      181 aattcgatcg ccgcgaccat cgatgatctc gcgccggcga cgtacacctt cactgccgag
      241 tacagcggcg actcgcgctt cgtgccggcg atccagtcga tccagcaggt cgtcatcgtg
      301 ccgccattcg gaccgccgcc gcatctcaat gccatcagct tcggcgggcc agtccagctt
      361 tcctggtatg ccacgctcaa tacggatcac tacgagatcg agcgcaacaa cggcgcagga
      421 tggattgcgg caggaacatc cgccagcgct gcattcacgg atctgggcgc gcctgcatca
      481 tcggcgcttc tttaccgcgt ccgtgccaag gccgcgaatg gcacggcctc cgagtacagc
      541 ttgaccgatc ttgcgctgac ctacgctttc actgatggca cgatccaggc gggcgtgacg
      601 gcggtgaaac gtgttcatgt caccgagctg cgcgatgctg ccaacgccgt ccgcgcgctg
      661 gccggcctcg gcgcaatgag ttgggcggaa ccgactccgg atctcgttcg cgtcactcac
      721 attaccgagc ttcgcacggc catcgaccag gcccgcgccg cgctccatct tccaaccctg
      781 acgcacatcg atccaacgct gacggcgggc acgagcccaa tccgcgccgc acatttcgag
      841 gacctccgcg catcgatgcg ctgatagtct cccgtgcgtg ggcgaacctg cactgatcct
      901 catcgacgtg caatacgcga tcgacgaccc gagctggggc aacgatcgga acaatcctga
      961 cgccgaggag aagatggcgg cactgctggc gcattggcgt gagcggcgtt ggcccatctt
     1021 ccacattcgt cacgcatcga ccgatccgaa ttcgacgtat cgtccaggcc agccgctctt
     1081 cgcattcaag aaagaagtga cgccgatcga cggcgagccg gtcatcgaga aatcgaccaa
     1141 cagcgcgttc atcggaacgc gccttgagga aatgctgcga tcacgcgggg tcgtgcaggt
     1201 cgtgatcgcc ggcgtgatca cgaacaactc cgtcgaggcc acggcccgga tgtccggcaa
     1261 cctcggcttc caaacgttcg tcgcatccga cgccaccgcc acgttcggcc gccccgattt
     1321 cgcgggacgt tggcgaaccg cggaggaggt tcacgcgatg tcacttgcga atctggctgg
     1381 ggagtacgcg actgtgctcg ggacggacga aatccttaac cggtagctgg cgtccatcga
     1441 gcgagaaccg caccacagag acacagagag cacagagagc atcactgcaa ctcggtgatc
     1501 tctgtgtctc tgtggttcat tggttctgag aaatgcggac tagcggccga ttacatacaa
     1561 atccgacagc aatcgcgtgt agctgtagac agtcgttgcg ccatccggcg taacgaggat
     1621 cgggccgacg ttgatcaggc cggaggggtc ggcgggcatt gcttcgcgcc agagggttcg
     1681 cgtgccggat gccagatcga tcttgtagag catcgccggg attttgccga acgtggccac
     1741 gaatagtgag cggccgtcgg cgctgaagtg cgtggggacg tcgccggtct tgagtgcggg
     1801 gacgggaagg ggagcgccgc ctgcgatcgg atagagatag aacttgtgat cggtcgcgcg
     1861 gccgagtacg taacgcccgt cgggcgtgac ttgcgtgccg ctgacgtttt cgggggtgat
     1921 ggcgcgcggg gcgcttccat cgagcgcttg aatccagagt ttgggcggtt ttccttcttc
     1981 gttaccctga aagaaaacct gcttgccgtc gggcatccag cgcgcgttgc ggtgattgat
     2041 gcggtctctc gtgatttggc gcggctggcc ggcgccggtc ggcagcatga cgatctgggc
     2101 gggattgcgg ttgcgcggta tcgacagcac ccactctccg tccggcgaca acgcctcgga
     2161 ggttccgtcg ccgaggcgga tggcggggga gccgtcgatg ccgcggatgt acacgccgaa
     2221 gatcgcgcca cctgcttcgc ccgattccga gaagacgatc gtctttccat ccgcggaaag
     2281 atcgcgcacg acggagtagt cgaaccacga gagatcgacc ggcttgtcgt ttccgcgaat
     2341 ggccacgatt ccggcgcgaa cgatctcgcg tgtgacaaga atgcgtccgt ccggcgcgac
     2401 gtcgtgcagc cagagcgggc cggcgctcgc agcgatcagc cgttctttgc cgcgcagcgt
     2461 caccgcgcga acggagcgcg acgtattacc gtcctcggat gagaagacga tctcgttacc
     2521 ggctgcggtc caggccagac cttcgatgct ctgcgactct ttcgtcagcg ccttgtgccc
     2581 tttgtccgcg agcgtgacca gaacgacgga gccgcggtcg tcgttgaaga atggatgatc
     2641 gagaagcccg atcgtgtcgc cggccggatt aaaacgcgga tggctgatcc atcccaccgt
     2701 gtcgtaaagg accgtgccga tcgggaactc caaccggcag cggccgttta cggtgcggac
     2761 gacggcgagc cgtccgtcgg gcgaccaatc ggcatactcg acgtcgtcga gaatctcgcg
     2821 cggcgcgccg ccggcgatcg ggacctgcgc cagcgtgccg ctctcgcgca cgtagccgat
     2881 cggatgccga tgcagcgaga tggccagttg tccgccgcgc gaaacggcca gaatgtcagt
     2941 gtcggcaccc atgtcgagat cgcgcgattc gggggaatcg gcgcgaacgg aaaagatcga
     3001 catcggcttt ccgctccagc gcgcgccgta gaagacggtc tggccgtcgg gcgagaagcg
     3061 tgcgttgaag atcgtgccgc tgcggaacgt cagccggcgg tacgacggcg gcggctgtgt
     3121 cccgcggcgc tgtccgatga ggtaggcgat cgcggcggcc gcgagaatca cggcagcgat
     3181 ggtcagcggc ttcaacagat tgcgcggacg aatgcgccac atcggcagga acgagacggc
     3241 ctgactggtc agcccggaga gtgagccgag gtcgaaggcg aggtcgcgcg cggactggaa
     3301 gcgctcgtcc ggattctttt ccagacagtg ccggacgacg cggtcgatcg cgccgggaac
     3361 gcggatattc aattcgaaga agtcgggtgg atcgtctttg aggatggagt tgagcgtctc
     3421 gatcggcgac tctgcgcgga acgcctgcac gcccgccagc atctcgtaaa ggatgacgcc
     3481 gaacgcgaag atgtcggatc ggttgtcgcc gctgccgccg cgcacctgct ccggcgccat
     3541 gtagccgatc gtccccatca agcgcccggg ctcggtcggc gtcgtcggca gcgtcagctc
     3601 gtgttcatcg ccgggcgtgg cgttcggcac ccgaggcccg gccagtttgg ccagaccgaa
     3661 gtcgaggatc ttgacgtggc cgtcgcgggt gatgaagaga ttctccggct tgaggtcgcg
     3721 atgaacgatg ttgcgctcgt gcgcggcggc gagtccgcgg gcgatctgcg cggcgtagtc
     3781 gagcgccttt cgcggcggaa tggcaccgtg acggatgcgc gcgcggagcg attccccttc
     3841 cagcaattcg gagacgatgt agttctggcc gccttcgttg ccgatgtcgt agatggccag
     3901 gatgttggga tggttgagca tcccggccgc tctcgcttcc tgctcgaacc gccgcagcgc
     3961 ctcgacgtcc ccggagagat tgcgcgtgag gaccttgacc gcgacgtcgc ggccgaggcg
     4021 ggtatcttgc gctcggtaca cctcgcccat cccgcccgcg ccgatggccg tgaggatctt
     4081 gtacggaccg agcatcgtgc ccgcagcaag catggccgtg aatcatgtca cgggatttgc
     4141 ctcgtgcggc tcgatagatt tgcggaatcg cagatccgtg gattagataa gtacgttagt
     4201 cagcttatag atcggtggag tggattaccg ataacctagg cactcatgaa acctgcacag
     4261 accatccaac tcacacccgg caaacgcgtt ctctttctga ccaaagatcc ggagctgatc
     4321 cggaagcagc ttcgcggcga gctcgatctt cagatgaagg atctgcgcgt cgaggacctg
     4381 ctcgacgaca tcaacaccga cgccatgacg ccggcgtggg tctgcttcag ccacaagccg
     4441 gaagacatcg ccgaggatgc gtacgcgggg ctgatcgtgg atgggaagcg gctgttcgag
     4501 cacgacgcgc tgatcaacgg caacttcgaa gtgatcgtca gcggttaccg caagggcgtc
     4561 ggcagctcgc gcgagacggc gacgcagtgc gaggtctgga gcggcatccg tatcgccgtg
     4621 gccgcgtcgt tcgcaccgat ccatgcgggc aacaacatca atcagggatt gctgatggcg
     4681 gaccacgcca tgctcctgcg gctgcaggca ggggaaggga tcccgatcga cgagttctgc
     4741 aaaggccgcg acgccatcac gcagctcatc atccgcgaag gcggtctctt cccgttcgcg
     4801 aaagcagtcg cgcgcggcga gattcaggtg ccggttccgg atacgccgaa gcgtccgatg
     4861 acgatggggg agaagattct ggcgaggcat gtgttggtgg ggggtggggg ggcgggggtg
     4921 gggagtggga gcaaaaccga ttcgtcagtc accggctccc aacccccact cccaactccc
     4981 cacaccccat acgttcaacc cggcgacgcc atcgtcgtgg aagtcgatgg cggttacacg
     5041 cacgagttca cgactgcgca ggttcactac ttcctcgagc aggagtacgg acctgactac
     5101 gcgatcaaga atccgcagaa gtttgcggtg ttcgaggatc acctcatcta tgccgacgac
     5161 gtcgcgcgca tgcggccgtt catggggaag atccagacgc tgcgcgacct gcaacgcgcc
     5221 tttcagcagc acaccggctg ccgtgatttt tcggcggtgg acaacatctc gcccggcatc
     5281 tgccacgaag ttgcgcgcga atacatcatc gatccgggcg acttcatcca ggccaccgac
     5341 agccacacct gcatgggcgg ggtcaacaac gctctcgcct ggggagtggg cgcgaccgag
     5401 tacgccgacc tggtgcatca ggggttcacc caggtcgagg tccccgagtc gatccgcttc
     5461 g
//
LOCUS       DATLVW010000300         7973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_382480, whole genome shotgun sequence.
ACCESSION   DATLVW010000300 DATLVW010000000
VERSION     DATLVW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382480"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VNN25_09275"
     CDS             <1..1253
                     /locus_tag="VNN25_09275"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HXH91760.1"
                     /translation="GCRSGSEDSDELRGAGLNWRSVPLETLGEFRNGLNYTTANDGVG
                     LRILSVKDFGDRSIPDVDGLSEIDPAGLPLEKALLRENDLVFVRSNGNKALVGRSMRI
                     DQNYDNLSFSAFCIRFRVTSSEVDARFLSYYFRSPLFRQTLSSLGQGTNINNLSQPLM
                     RQLPIPCPELPIQEQVVTVLSTYDDLIENNRRRMVMLEEGSRQLYREWFVRLRFPGHE
                     HARIVDRVPEGWRIGTFGSIAIDVRERVAPNDVEGATPYIGLEHIPRRSIALVSWGRA
                     DEVTSTKFRFREGDIIFGKIRPYFHKVGIAFVDGVASSDSIVIRPEAEDLRALLLMTV
                     SSDPFVAVAAQGMREGSKMPRADWKLMQQYPVAVPPPGLLGSFTETIDNIVHQLKILT
                     FQNQKLRAARDLLLPRLLSGEITV"
     gene            1250..1771
                     /locus_tag="VNN25_09280"
     CDS             1250..1771
                     /locus_tag="VNN25_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH91761.1"
                     /translation="MSDKKVVIIAGPNGAGKTTFAREFLPAEAGLPVFVNADLIAAGL
                     SPFDPAAAAIRAGRIMLQEIDAHAAGGRSFAFETTLAGHTYVRRLAAWRASGYFVELI
                     FLSLPSAEEAIRRVAERVRQGGHDVAPDVIRRRFDAGMRHFLQYYRHRVDEWMLFDNS
                     GRMPRLLEQGHIA"
     gene            1768..1995
                     /locus_tag="VNN25_09285"
     CDS             1768..1995
                     /locus_tag="VNN25_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91762.1"
                     /translation="MSETVSEVTVSKSASADMQAVPRALLRAARRARAIARETGTLLI
                     IVEDGILKELAPDDPDFDSLERTTSTIHDGD"
     gene            2026..5409
                     /locus_tag="VNN25_09290"
     CDS             2026..5409
                     /locus_tag="VNN25_09290"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HXH91763.1"
                     /translation="MALTAINTEDRLVQQTFATHLREVLGWETVYTHNSETFGPNGTL
                     GRDSERDAILKRELRSALERLNPKIPESARESAVEKLTRNDLSRSLIQHNRDFYTFIR
                     EGVPVEWADESGVKRYARARVLDFRDITENRFLAVRELKIQGMHVPHYNRRADLVCFV
                     NGLPLVFIELKAVYKNIRAGFDENLTDYLNESIPHAFHHNAFLIVSNGDRARYGSITS
                     KWDHFAEWKRDRESDKGRVDAEALLDGMLPKDRLLDIVENFILFDDSRAGGTRKIVAR
                     NHQVLGVNNAVASVERQEELKREYPPGARLLEYRAPVSEGRMIADAPVESLVRRQKNE
                     LVLPQRAHPDLGRLGVFWHTQGSGKSYSMAFFSEKVRRAVPGNFTFLIMTDREDLDDQ
                     IWRTFIGCGITDEMTPRAGSGKDLKALLAGNHRYVFSLIHKFNQPVAEPYSDRDDIIV
                     ISDEAHRTQMGRFARNMRQALPNASFIGFTGTPILKHDHLTNRIFGGYVSRYDFKRSE
                     EDGSTVKLIYESRGEKLGLARLDLNDRIAEAVEVAELDDDQTALIEKLLGKDYEVITA
                     DDRLDKLADDFVEHCSTRWETGKSMFVCIDKITCGRMFQRIEKRWQARLLQVKAQIAA
                     AESDLAMASDPDDRERIERELDHARGRERWMESTIVEIIISEAQNEVRDFAKWGVDIV
                     PHRVRMKRGFETPDGKRMSVEDAFKDPSHPFRVAIVCAMWLTGFDVESLATLYVDKPM
                     KAHTLMQAIARANRIFPGKDCGVIVDYNGMLKSLRKALADYALGDEDEGETSDEPAAP
                     IEELVGALIDAIEAAERHLSTLGFDATRLHGATGFTKIEALRDAVDACYTSDEAKRRF
                     EIIARQVFIRMKALLMEPAAWTYAERHDNLEAIYKKLQERRDTADVTTLLKALHRIVN
                     EAIRAQEAGDDHAEGLTVDLSGVDFEKLREEFARKVRRKHAALQDIRDIVEQKLAAML
                     AANPLRMDYYRRYQEIVADYNREKDRVTVEATFAALVDLVASLDDEQQRAAREGLGET
                     ELAFFDLMRRDTLSAADRERLKQVSRHLLSTLQALLSRMPRWTENESTQAEVQTSILD
                     ELYETLPRPPFEEDEINDISERVYQFVWKKSSRSEPLVF"
     gene            complement(5491..6780)
                     /locus_tag="VNN25_09295"
     CDS             complement(5491..6780)
                     /locus_tag="VNN25_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91764.1"
                     /translation="MPQVVILSLFLGLVTGTQTVALRVGPAVKSVRIELGGREAARLT
                     GEPWTAKIDFGSALLPRELVAIAYDSAGKELARASQLTNLSPPPAELDIIIHTEEQRP
                     AEAELVGRHRIHRHAEAATLLLDGKELKLSGSFRAKLPPLEPSRTHVLSAEMRFEDGL
                     VARRDLVLEGEFSRDTGNELSAVLVSVTGSEPTNLDGCFSANGITLRASAIEKTDALV
                     IMVKDPESRDLLARLLLDANRREFILGADTTERMLWPVARPFNAPGEPVAIAFPQSND
                     RGGSGSRTRATVSWLLTLGLSPKPNANEPRQYTDAVAVAGMNARERGQRRAVVLLLGK
                     KADQSLYAAKVVRRYLEEVGVPLLVWSPDGPRPDLAESWGPVEDVSTAPALMAASARL
                     EKLLAQQRIVWLAADPLTALRAQTAERCGLTPVAHPR"
     gene            6901..>7973
                     /locus_tag="VNN25_09300"
     CDS             6901..>7973
                     /locus_tag="VNN25_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXH91765.1"
                     /translation="MKSRHLPALFALALSIVLPLRGDCPFGLLSPSHYDANVRPGDIA
                     NGDFNKDGYLDLAVVNRQTNTISILLGAAGGTFGAPSQITLASPTQADILAADFNKDG
                     NIDLVIAEGGTYPSVLPQVEVFLGYGDGTFHAVAFTGQQYVRNPTRMVLGDFDNDGLV
                     DVAVATQDGEFSSMKSLGNGILAQISENSTAESGGNPTGLARGIASGDFDGDGKLDIA
                     VSEVISKKVYLFFGNGDGTYFKSPNVIDLPAEQNEPWDIQSGDFNGDGKADLAIVMRN
                     PYGGPDPPPLKIALSNGVARTFATPVDYGQLNAGDQAVVQDIDGDGKLDVLVGDAFGI
                     SIFRGNGDGTFAAQQKIGTWALGL"
BASE COUNT         1626 a         2410 c         2412 g         1525 t
ORIGIN      
        1 gaggctgccg atctggcagc gaagattcag acgaacttcg aggagctggc ttgaattggc
       61 ggtccgttcc gcttgaaacg ctgggcgagt ttcgcaacgg tttgaactac accaccgcga
      121 atgacggggt aggtttgcgg atactttccg tgaaagactt cggtgaccga tcgattcccg
      181 acgtcgacgg cctttcggag attgacccgg ccggactgcc tctcgaaaag gcattactcc
      241 gtgaaaacga cctcgtcttt gtccgttcca atggcaacaa ggctttggtc ggacgctcaa
      301 tgcgtatcga tcagaattac gacaatttat ccttctctgc gttttgtatc cgcttccgag
      361 tcacgtcatc agaagtggat gcgcgtttcc tctcttacta ctttcgatcg cctctgttcc
      421 ggcaaacgct ctcaagcctg ggacagggta cgaacattaa caacctcagt cagcccttga
      481 tgagacaact accaataccc tgcccggagc ttccgattca agaacaagtt gtcacggtgc
      541 tgtccaccta cgacgacctg attgagaaca accgtcgccg gatggtgatg ctggaggagg
      601 ggtcgcggca gctctatcgc gaatggttcg tccgcctccg cttccccggc cacgagcatg
      661 cgcgaatcgt tgaccgcgtc ccggagggtt ggagaatcgg gactttcggc agcatcgcga
      721 tcgacgtgcg cgagcgggtt gcaccgaacg atgtcgaagg ggcgacacct tacatcgggc
      781 ttgagcatat accgcgccga tcaattgcgc tcgtatcatg gggacgcgcc gatgaagtca
      841 ccagcacgaa gttccgtttc cgtgaaggcg acatcatctt cggaaaaatc cgaccgtatt
      901 tccataaggt cggtattgca ttcgtggatg gtgtcgcatc gtccgattcg atcgtcatcc
      961 ggcctgaggc cgaagacctt cgtgcactgc ttctaatgac tgtgtccagc gatccgtttg
     1021 ttgcagtcgc agcccagggc atgcgggaag gctcgaaaat gcctcgcgcc gactggaagt
     1081 tgatgcagca atacccagtt gccgtgcctc ctcccggtct gctcggatcg tttactgaaa
     1141 cgatcgataa catcgtccat caattgaaaa tactgacctt tcaaaaccaa aagctccgcg
     1201 ccgcacgtga tctactcctt ccccgcctct tgagcggaga gatcactgta tgagcgataa
     1261 gaaggttgtc atcatcgccg gtcccaacgg tgcgggaaag acgaccttcg cgcgggagtt
     1321 tctgccggcc gaggcggggt tgccggtgtt tgtgaatgcg gatctcatcg cggccggact
     1381 ctcgcccttc gatccggcag cggctgcgat tcgtgctggt cggatcatgc ttcaggagat
     1441 cgacgcccac gctgccggcg gacggagctt cgccttcgag acgacgctgg ctggtcacac
     1501 gtacgtccgc cgcctcgccg catggcgtgc ctccggctat ttcgttgaac tcatctttct
     1561 ctcacttccc tcggcggaag aggccatccg ccgtgttgcg gaacgggtcc ggcagggtgg
     1621 ccacgacgtg gcgccggacg tgatccggcg cagattcgac gcaggtatgc gacacttcct
     1681 tcagtactac cggcatcgtg tcgatgagtg gatgctcttc gacaacagcg gccggatgcc
     1741 acgtctgctc gaacaaggac acatcgcgtg agcgaaaccg taagtgaagt gacggtatcg
     1801 aagtcggcca gcgccgacat gcaggccgtc cctcgcgcac tccttcgcgc tgcccggcgc
     1861 gcacgcgcga tcgccaggga aacggggacg ctcctcatca ttgtcgagga tggcatcctc
     1921 aaagagcttg ctcccgacga tcccgatttc gattcactgg agcggactac cagcacgatc
     1981 cacgacggtg attgatggag gcattcgctc gatgaggtca gcccggtggc tctgacggcg
     2041 atcaacaccg aagaccggct cgtgcagcag accttcgcca cgcacctgcg cgaggttctc
     2101 ggttgggaaa ccgtctacac ccacaacagc gagacgtttg gtccgaatgg aacgctcggc
     2161 cgcgattccg agcgtgatgc gatcctgaag cgcgaattgc gcagtgccct ggagcgtctc
     2221 aatcctaaga tcccggaatc ggcccgcgaa tcagccgtcg agaaactgac gcgcaacgat
     2281 ctctcccgat ccctcatcca gcacaatcgc gatttctaca cgttcatccg cgaaggcgtg
     2341 cccgtcgagt gggccgatga gtcgggcgtg aagcgctacg cacgagcgcg tgtgctcgat
     2401 tttcgcgaca tcacagagaa ccgattcctg gcggtgcgcg agctgaagat tcagggaatg
     2461 cacgtcccgc actacaaccg ccgtgccgat ctggtctgct tcgtcaacgg actgcccttg
     2521 gtcttcatcg agttgaaggc ggtctacaag aacatccgtg ccggtttcga tgaaaacctc
     2581 accgactatc tcaacgagag catcccgcac gcctttcatc acaatgcgtt tctcatcgtg
     2641 agtaatggcg accgcgcccg ctacggctcg attacgagca agtgggacca cttcgcggaa
     2701 tggaaacgtg atcgcgaatc cgacaaaggg cgtgtggacg ccgaggcgtt gctggacggg
     2761 atgctgccaa aggaccggtt gctcgacatc gtcgagaact tcatcctctt cgatgacagc
     2821 cgcgccggcg gaacgcggaa gatcgtggcg cgcaatcacc aggtgctcgg cgtcaacaat
     2881 gccgttgcct cggtggagcg gcaggaagag ctgaagcgcg agtatccgcc gggcgcgcga
     2941 ctactcgaat atcgcgcgcc ggtttccgag ggaaggatga tcgctgacgc gccggtcgag
     3001 agcctcgtgc gccgtcagaa gaacgagctc gttcttccac agcgcgctca tcccgatctc
     3061 ggccgcctcg gcgtgttttg gcatacgcag ggaagcggca agtcgtactc gatggcgttc
     3121 ttctcggaga aagtgcgccg ggccgttccc ggcaatttca cgttcctcat catgaccgac
     3181 cgtgaggacc tcgacgatca gatctggaga acgtttatcg gctgcggcat caccgacgaa
     3241 atgacaccac gagcgggaag tgggaaagac ttgaaggcgc tgctggccgg caatcatcgc
     3301 tacgtcttca gcctgatcca caagttcaac cagccggtgg cagagccgta cagcgaccgc
     3361 gacgacatca tcgtcatctc ggacgaagcg catcgcacgc agatgggacg gttcgcgcgc
     3421 aacatgcggc aggcgctgcc caacgcctcc ttcatcggat tcacgggtac gccgattctc
     3481 aagcatgacc atctaacgaa tcggatcttc ggcggctacg tttcccgcta tgacttcaag
     3541 cggtcagaag aggatggatc gacggtcaaa ctgatctatg agagccgagg tgaaaagctc
     3601 ggtctggccc gcctcgatct gaacgaccgg atcgccgagg cggtggaggt ggcagagctc
     3661 gacgacgacc agacggcgct gatcgagaag ctgctcggga aagactatga agtcatcacg
     3721 gcggatgatc ggctcgacaa gctggcagat gatttcgtcg agcactgcag cacgcgatgg
     3781 gagacgggta agtcgatgtt cgtctgcatc gacaagatca cctgcggcag gatgttccag
     3841 cggatcgaga agcgatggca agcacgcctt ctccaggtca aagcacagat cgcggcggcc
     3901 gaatctgatc tggcaatggc aagtgatccg gacgatcgcg aacgaatcga gcgagaactt
     3961 gaccacgctc gtggccgcga acgctggatg gaatcgacga tcgtcgagat catcatcagt
     4021 gaggcacaga acgaggtgcg cgacttcgca aagtggggcg tggacatcgt tccgcatcgc
     4081 gtccggatga aacgcggctt cgagactccg gacggtaagc gcatgagcgt cgaggatgct
     4141 ttcaaagatc cgagccatcc ctttcgcgtc gcgatcgtct gcgcgatgtg gctgaccggg
     4201 ttcgacgttg agtcgctggc cacgctctac gtcgataagc cgatgaaggc gcacacgctg
     4261 atgcaggcca tcgctcgtgc caatcgcatt ttccccggca aggattgcgg agtaatcgtc
     4321 gattacaacg ggatgctgaa gagtcttcgc aaggcactgg ctgactatgc gctcggcgac
     4381 gaagacgaag gggagacatc cgatgagccg gccgcgccga tcgaggaact ggttggggcc
     4441 ttgatcgacg cgatcgaggc cgccgaacgg cacctctcga cgctcggctt cgatgcgacg
     4501 cggcttcacg gcgctactgg gtttacgaag atcgaggcgc tacgcgatgc ggtcgacgcc
     4561 tgctacacct cggacgaagc gaaacgccga ttcgagatca tcgcgcggca agtcttcatc
     4621 cgcatgaagg ccctgctcat ggagcccgcc gcgtggacgt acgcggagcg tcacgacaat
     4681 ctcgaggcca tctacaagaa gctacaggag cggcgcgata ccgcagacgt tacgacgctc
     4741 ctcaaagcgc tgcaccgtat cgtcaatgaa gcgatccgcg cacaggaagc tggagacgat
     4801 cacgccgagg gcctcaccgt cgatctcagc ggagtcgatt tcgagaaact gcgcgaagag
     4861 ttcgcacgga aggtccggcg aaaacatgcg gcactccagg acattcgcga cattgtcgag
     4921 caaaagctgg ccgcgatgct cgctgcgaat ccgctcagga tggactacta ccggcgctac
     4981 caggagatcg tcgccgatta caaccgcgag aaggaccgag ttacggttga agcaacgttt
     5041 gccgcactcg tcgatctcgt cgcttcactc gatgacgaac aacagcgcgc cgcacgtgaa
     5101 ggcctgggcg agacggagct cgctttcttc gatctgatgc ggcgagacac gttgagcgcg
     5161 gcagatcgtg agcgcctcaa gcaagtcagc cggcatctgc tctccactct ccaggctcta
     5221 ctgtcgcgca tgcctcgatg gacggaaaac gaatcaacgc aggccgaggt gcaaacgtcg
     5281 attctcgatg agctgtacga gactcttccg cgtccgcctt tcgaagaaga cgagatcaac
     5341 gatatctcgg aacgcgtcta tcagtttgtc tggaagaagt cgtccagatc tgagcccctg
     5401 gtgttttaac caaagcctgt gcagaactcg ggaccaccga agcacagccc gcgccgagac
     5461 gatccgtgcc tatctgcgtc atctgcggat tcaccgagga tgcgcgacag gcgtgagtcc
     5521 gcaacgttct gcggtttggg cgcggagtgc ggtgagggga tcggcggcca gccagacgat
     5581 gcgttgttgc gcgagcagct tctccagccg cgctgatgcg gccatcagtg cgggcgccgt
     5641 tgatacgtcc tcgaccggac cccacgactc cgcgagatcg gggcgcggtc cgtcgggcga
     5701 ccagaccagc agcggtacgc cgacttcttc gagatagcgg cgcacgacct ttgccgcgta
     5761 gaggctttga tcggccttct tgccgaggag gaggacgacg gcacggcgtt gcccgcgctc
     5821 gcgcgcgttc atgccggcga cggcgacggc gtccgtgtac tggcgaggct cgttcgcatt
     5881 cggtttaggt gacagaccga gcgtcagcaa ccacgacacc gtcgcgcgcg tgcgtgaacc
     5941 gctgccgccg cggtcgttcg actgcggaaa ggcgatcgca actggctcac ccggagcatt
     6001 gaacggacgg gcgacgggcc agagcattcg ctccgtcgtg tccgcgccga ggatgaattc
     6061 gcggcggttg gcgtcgagca gaaggcgcgc cagaagatcg cgggactcgg gatctttgac
     6121 catgatcacc agcgcgtccg ttttctcgat cgcgctcgca cgcagcgtaa ttccgttcgc
     6181 ggaaaagcaa ccgtcgaggt tcgttggctc gctgccggtg accgagacga gaacagcgct
     6241 caactcgtta ccggtgtcgc gcgagaactc cccttcgaga acgagatcgc gccgcgccac
     6301 cagcccgtct tcgaatcgca tctcggccga cagcacatgc gtacgcgatg gctcgagcgg
     6361 cggcagcttg gcgcgaaagc tcccgctcag cttcaattcc tttccatcca ggagcagcgt
     6421 tgcagcctcg gcatgcctat gaattcgatg ccgtcccacg agctccgctt cggccggccg
     6481 ctgttcctcg gtatggatga tgatgtcgag ctccgcgggc ggcggcgaga gattggtgag
     6541 ctgcgacgcg cgcgcaagct ccttccccgc gctgtcgtag gcgatggcca ccagctcccg
     6601 cggcagcagc gccgagccga aatcgatctt cgccgtccac ggctcgccgg tgagcctggc
     6661 cgcctcgcgt ccgccgagct cgatgcgcac cgacttcacc gccggcccga cgcgcagcgc
     6721 gacggtctgc gtgccggtaa ccaacccgag gaagagcgag aggatgacta cctgcggcat
     6781 cgcggtaggg tattactgtt tgaccacaga ggcgcagagc gcacagacgc gtgatgcttg
     6841 tcacgaccag cgctggcgaa gcatctgtta ttctccgcgc aggtccgatc tccatcccta
     6901 atgaaatcac gtcaccttcc cgctctcttc gctctcgctc tgtcgatcgt gttgccactt
     6961 cgcggcgact gtccgtttgg tctcctctcg ccttcgcact acgacgcgaa cgtgagaccc
     7021 ggtgacatcg ccaacggcga tttcaacaaa gacggctatc tcgatctcgc cgtcgtgaat
     7081 cgtcagacca atacgatctc catcctcctc ggtgcggcgg gagggacgtt cggagctccg
     7141 tcgcagatca cgcttgcctc cccgacacag gcagatattc tggcggccga cttcaacaaa
     7201 gacggcaaca tcgatctcgt cattgccgag ggtggaactt acccctcggt actccctcag
     7261 gtggaagtct tcctggggta cggcgacggc acattccacg cggtcgcgtt tacggggcag
     7321 cagtacgtcc gtaatccgac gcgcatggtt ctgggcgact ttgataacga cggtctggtc
     7381 gacgtcgcgg tcgcgacaca ggacggcgag ttctcttcca tgaagagcct cggcaacggc
     7441 atcctggctc agatatccga aaacagcacc gccgaatcag gtggcaaccc cacgggcctc
     7501 gcgagaggca tcgcctcggg tgatttcgac ggcgacggca agctcgacat cgctgtcagc
     7561 gaagtcatct cgaagaaggt ctatctgttt ttcggtaacg gcgatggaac ctacttcaag
     7621 agtccgaatg tgatcgatct accggccgag cagaatgagc cgtgggatat ccagtcgggc
     7681 gatttcaacg gtgatggcaa ggctgatctg gccatcgtca tgcgaaatcc ttatggtggg
     7741 cccgacccac caccgctgaa gatcgcgctg agcaacggtg tggcgcgtac gttcgcgact
     7801 ccggttgact acggccaact gaacgccggc gatcaggctg tcgttcagga catcgacggc
     7861 gatgggaaac tcgacgtgct ggttggtgac gcattcggta tcagcatctt ccgcggaaac
     7921 ggcgatggga catttgccgc gcagcagaaa atcggaacct gggcgctcgg act
//
LOCUS       DATLVW010000301         4444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_382493, whole genome shotgun sequence.
ACCESSION   DATLVW010000301 DATLVW010000000
VERSION     DATLVW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382493"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VNN25_09305"
     CDS             <1..231
                     /locus_tag="VNN25_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91766.1"
                     /translation="HTIRMITTDGRLITLAGVPGIAGFADGVASSARFAGPVGIRIAP
                     DGSLLIADTSNNAIRRLSIISVPDTRERSVRH"
     gene            340..735
                     /locus_tag="VNN25_09310"
     CDS             340..735
                     /locus_tag="VNN25_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH91767.1"
                     /translation="MQYPTVSRGRNWALWTIQILLALLYLFSGSMKFVMPVAKMNEGA
                     VKFPGLFYHFIGVCEVLGGLGLILPGLTGIRRGLTPLAAVGLLIIMIGATTVTLMDGM
                     GMVALLPFVVGVMVAIVAYGRWYWLRGVA"
     gene            885..4238
                     /locus_tag="VNN25_09315"
     CDS             885..4238
                     /locus_tag="VNN25_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXH91768.1"
                     /translation="MKKLVAAFVLFVATSVFAAGHTLYQNPSLSRTRIAFAYAGDIWT
                     VARDGGVADRLTTYPGVESDPRFSPDGKWVAFTGEYDGNTDVYVVPAEGGEPKRLTFH
                     PGADQVVGWTSDGTKVLFRSSRQSARGYGQLFTIGLDGGLPDLVPLPHADEGSFSPDN
                     SRIAYQPISQWQPDWKRMHGGQTGQIWIARMSDSSIERVPHPGSNDHAPAWMGDTVYF
                     ISDRGSRTGTTSIFAYDTRSKAVSEVVKNEGLDIKSMSAGPDALVYEKFGTIFLLDPK
                     TRVPKEVPIQVRGDFLGLRARYAPAADSIRTGHISPTGARAVFEARGEIITVPVEKGD
                     ARNLTNTVGVMERDPAWSPDGKSIAYFSEESGEYALVIRDQKGDRPGRKIDLGEPASF
                     YYRPVWSPDSTKVAYSDRRLNLWYVDVATGARVKVATNPIGFNGDVLEPRWSPDSRWI
                     TYARYLPNLVRAVFVFSLESQKSSQITDGLSDVIYPVFDRNGKYLYFAASTDIGRGIS
                     WADLSGIDSVATRNVYAAVLRNDLPSPLAPESDEEKAADDKSKDAKKDDKEVKKDDKE
                     PEKKDAKEPEKKDDKDKKDAADKPKVTPVRIDLDGIGQRIIALPIPNGNIQGLAAGKD
                     GILFVGIAPPSFFGSDDEGVNVRRFDLEKRKVKDFLSGVNAFDVSANGEKVLYQRKKD
                     WAIASSADEPKAGEGSLKLGGIQIRVDPRAEWRQMYRDVWLGERDHFYDENHHGLNLE
                     AAKKLYEPYLDAVANRADLNYLFREMLNQLTIGHMFIRGGDMLETKSVPGGLLGCDYA
                     VDNGRYRFSRVYSGENWNPELQAPLTQPGVNVKAGDYLISVAGRDLRASDDVYSRFEN
                     TAGKQVAIRVGAKPDGSDAREVIVVPVASESALRGRAWIEDNRRKVDQLSGGRLAYVY
                     IPNTGGTGFKSFNRDFFVQTGKEGAVIDERFNQGGLLADYVVNMLTRPQLSAIRFRYA
                     EKDVAVPGGAIYGPKAMLINSMSGSGGDAMPWYFKKMKVGPLVGTRTWGGLVASQTGP
                     RLMDGGTYTAPDAAVYGLNGNWEVENAGVSPDIEVELDPAEWRKGHDTQLEMAVKTLM
                     EQVMQHPRTEPVRPAFPVYERCCGLDTKH"
BASE COUNT          899 a         1325 c         1421 g          799 t
ORIGIN      
        1 cacacgatcc gcatgattac gacagacggc aggctgatca cgctcgccgg cgtccccggc
       61 atcgccggct ttgccgatgg agtggcgtcg tctgcccgat tcgcaggtcc ggtcgggatt
      121 cgcatcgcgc cggatggttc cctgctcatt gccgatacgt ccaacaacgc catccggcga
      181 ctctccatca tttcggtgcc ggatacgaga gagcggagcg ttcgacactg atattctcgg
      241 gagtcgtttg tcgggggtcg ggagtcggag aagccaggat tgatcgtgct tcgactcccg
      301 acccccgacc ccctacaccc gacaatcgag gagatcgaca tgcaatatcc gacagtgagc
      361 cgaggacgga actgggcgct ctggacgatt cagatcctgc tggccctgct ctatctcttt
      421 tcaggatcga tgaagttcgt gatgccggtg gcgaagatga acgaaggggc ggtcaagttc
      481 ccgggtctgt tctatcactt catcggtgtc tgtgaagtcc tgggcgggct tggcctgatt
      541 ctccccggcc tgaccggaat ccgccgcgga ctgacaccgc ttgccgccgt cgggctgctg
      601 atcatcatga tcggcgcaac gacggtgacg ctgatggacg gcatggggat ggtcgcgctg
      661 ctcccgttcg tcgtcggcgt gatggtggcg atcgtcgcgt acggccggtg gtactggctg
      721 cgcggggtcg cctgacagag cttaaccaca gagacacaga ggacacagag gtttctcggt
      781 gacctctgtg tctctgtggt aatcgccttc tgtcaacctt ctccctcttc catcgtttga
      841 atgcagtgca actcctatac ttcccattca cgaaaagagg gtccatgaaa aaacttgttg
      901 ctgccttcgt actcttcgtt gccacttcgg tgttcgctgc cgggcacacg ctctaccaga
      961 atccttcgct gagccggacg cggattgcgt ttgcgtatgc gggggacatc tggacggtcg
     1021 cgcgcgacgg cggcgttgcg gatcggctca cgacgtatcc gggggtggag agcgatccgc
     1081 ggttctcgcc ggatgggaag tgggttgcgt tcaccgggga gtacgacggc aacacggacg
     1141 tctatgtcgt gcctgccgag ggcggcgagc cgaagcggtt gacgtttcat cctggcgcgg
     1201 atcaagtggt tggatggacg agcgacggga cgaaggttct ctttcgttcg tcgcgccaga
     1261 gcgcacgtgg ctacggccaa ctcttcacga tcggactcga tggcgggcta ccggatctgg
     1321 tgccgctgcc gcatgcggat gagggctcct tctcgcccga caattcacgc atcgcctatc
     1381 aaccgatctc gcagtggcag ccggactgga agcggatgca cggcggacag accgggcaga
     1441 tctggatcgc gcggatgtcc gactcgtcga tcgagcgcgt tccgcatccc ggctcgaacg
     1501 atcacgcgcc ggcgtggatg ggcgacaccg tctacttcat ctccgaccgc ggcagccgca
     1561 ccgggacgac gtcgatcttc gcgtacgaca cgcgcagcaa ggcggtttcc gaggtggtga
     1621 agaatgaggg gctggacatc aagtcgatgt ccgcggggcc cgacgcgctc gtgtacgaga
     1681 agttcggaac gatctttctg ctcgatccga agacgcgcgt gccgaaagaa gtgcctatcc
     1741 aggtgcgcgg cgacttcctc ggattgcgcg cacgctacgc gccggcagcg gactcgatcc
     1801 ggaccggaca catctcgccg accggcgctc gcgcggtgtt cgaagcgcgc ggcgagatca
     1861 tcacggtccc tgtcgaaaag ggagacgcgc gcaacctgac gaacacggtg ggagtgatgg
     1921 agcgcgatcc cgcctggtcg ccggacggca agtcgatcgc gtacttctcc gaagagtcag
     1981 gagagtacgc gctggtaatc cgtgatcaga aaggggatcg tcccggccgc aagatcgacc
     2041 tcggcgagcc ggccagcttc tactaccggc cggtctggtc gcccgacagc acgaaggtcg
     2101 cgtactccga ccgccggctg aacctctggt atgtcgacgt ggcgaccgga gcgcgcgtga
     2161 aggtggctac caatccgatc ggcttcaacg gcgacgtatt ggagccgcga tggtcgcccg
     2221 acagccgctg gatcacctat gcccgttacc tgccgaacct cgtccgcgcg gtattcgtct
     2281 tttccctcga gtcgcagaaa tcgtcgcaga tcacggacgg gctcagcgac gtcatctatc
     2341 cggtgttcga ccgcaacggc aagtacctct acttcgcggc cagcaccgac atcggccgcg
     2401 gtatcagttg ggccgacctc tccggtatcg acagcgtggc cacgcgcaac gtctacgcag
     2461 ccgttcttcg caacgatctt ccttcgcccc tcgcgccgga gagcgacgag gagaaggcgg
     2521 ccgatgacaa gagcaaggat gcgaagaaag acgacaaaga ggtgaagaag gacgacaagg
     2581 agcctgagaa gaaggacgcg aaagagccgg agaagaaaga cgacaaagac aagaaggatg
     2641 ctgcggacaa accgaaggtg acgccggttc gcatcgacct cgacggcatc ggccagcgaa
     2701 tcatcgccct gccgatcccg aacggaaaca tccagggtct cgcggcgggc aaggacggaa
     2761 tcctcttcgt cggcatcgct cctccgtcat ttttcggtag tgacgatgag ggcgtcaacg
     2821 tccgccgttt cgatctcgag aagcggaagg tgaaggactt cctctccggc gtgaatgcct
     2881 tcgacgtgtc ggcgaacggg gagaaggttc tctatcagcg aaagaaggat tgggcgatcg
     2941 cgtcatcggc tgacgagccg aaggcagggg agggatcgct caagctcgga ggcatccaga
     3001 tccgcgtcga tccccgcgcc gaatggcggc agatgtatcg cgacgtctgg cttggcgagc
     3061 gcgatcattt ctacgacgag aatcatcacg gactgaatct cgaagcggcg aagaagttgt
     3121 acgagccata cctcgacgcg gtcgcgaacc gcgccgatct gaactatctc ttccgcgaga
     3181 tgcttaacca gttgaccatc ggccacatgt tcatccgcgg cggcgacatg ctcgaaacga
     3241 aatcagtccc gggcggcctc ctcggctgcg actacgccgt cgacaacggg cgttaccgct
     3301 tctctcgcgt ttacagcggc gagaattgga atcctgagtt gcaagcgcca ctcacgcaac
     3361 cgggtgtgaa cgtgaaggcc ggcgattacc tcatctcggt cgccggccgc gatctgcgcg
     3421 ccagcgacga tgtctactcg cgcttcgaga acacggcggg caaacaggtg gccattcgcg
     3481 tgggcgcgaa gcccgacgga tcggatgcga gagaggtcat cgtcgttccg gtggctagcg
     3541 agtcggcact gcgggggcgc gcctggatcg aggacaaccg ccgcaaggtc gatcagctca
     3601 gcggcggacg cctcgcctac gtctacatcc cgaacacggg cggcaccggc ttcaagagct
     3661 tcaaccgcga cttcttcgtg cagacgggga aggaaggagc ggtcatcgac gagcgcttca
     3721 atcagggagg actgctcgcc gactacgtgg tgaacatgct cacgcgcccg caactcagcg
     3781 ccatccgatt ccgctacgcc gagaaggatg tggccgttcc gggcggcgca atctacggtc
     3841 cgaaggcaat gctcatcaac agcatgtcgg gaagcggcgg cgatgccatg ccgtggtact
     3901 tcaagaagat gaaagtgggt ccgctcgtcg gcacgcgcac atggggcggc ctcgtggcat
     3961 cgcaaaccgg accgcgcctg atggacggag gcacatacac cgcccccgac gccgcggttt
     4021 acggtctgaa cggcaattgg gaagtcgaga acgccggcgt gtcccccgac atcgaagtcg
     4081 agctcgaccc ggccgaatgg cggaaaggac acgatacgca gctcgagatg gcggtgaaga
     4141 cgctcatgga gcaagtgatg caacatcccc gcaccgagcc ggtccggccg gcatttcccg
     4201 tttacgagag atgctgcgga ctggatacca agcactgaga gtgcgctgga tcaggggaag
     4261 agatcagaga caagagatta gagacatcag agatcagggg ccggagatca gaggccaaga
     4321 tgagagatca gggatcaacc agagaccaga gaaccagaaa gggagtcatc tcttatctct
     4381 tgtctcttgt ctctgctctc ttgctctctt gatcatcggc cagagatcag agacaaggcc
     4441 atga
//
LOCUS       DATLVW010000302         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_261141, whole genome shotgun sequence.
ACCESSION   DATLVW010000302 DATLVW010000000
VERSION     DATLVW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261141"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VNN25_09320"
     CDS             <1..469
                     /locus_tag="VNN25_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXH91769.1"
                     /translation="ANLGIWATVLMLRRHQYAGERVDDFEGLSKRAPLWAFAMVIFLL
                     SLGGIPPTAGFIGKYFLFAAAVQAGFGWLAVIAVLMSAVSMFYYLRLVVAMYLREGQE
                     AEFAITTPLRIVAGICLVVTLALGIVPSSLIDQAVLSSSHVAAHAALTPHAGR"
     gene            456..704
                     /locus_tag="VNN25_09325"
     CDS             456..704
                     /locus_tag="VNN25_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXH91770.1"
                     /translation="MPDDKDSDSGKKIRQNETLLEASSIGLMFPIAIGLGYLWGWGMD
                     KFFHTSPWLTWIFTGFGVIAAFINLFRIGLRNDDGSSG"
     gene            718..1077
                     /locus_tag="VNN25_09330"
     CDS             718..1077
                     /locus_tag="VNN25_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HXH91771.1"
                     /translation="MLGRIRRIALAACGVFAIPVLVLLGWRGLIGLTCSGLVTMINFL
                     WLEEIVETLLQPAQHRHLGRVTIRTLSRFALVGAALLVTIFVARVNAASVLLGFSIVV
                     VGIMGEAVYSTVRSFSR"
     gene            1084..1791
                     /gene="atpB"
                     /locus_tag="VNN25_09335"
     CDS             1084..1791
                     /gene="atpB"
                     /locus_tag="VNN25_09335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXH91772.1"
                     /translation="MEHESSILYAPFNTLWHSVQHRFAWDVPDHVIMALLVLTISCVL
                     FPLMSRRISRENPSHFQQLLELVVGGLRDLLRDIIGHDGDRHLYIIGSFAVFIFLSNI
                     FALFFFLQPPTSNPNTTFGLALCAFVYYNYQGIKTQGIVHYLKHFMGPMLILAPLMIP
                     IEIIGHLARILSLGMRLFGNIFGEHAATGIFMGMLPFVLPWPMMGLGIFGAFLQTFVF
                     IMLTMVYIGGATVVEEH"
     gene            1857..2174
                     /locus_tag="VNN25_09340"
     CDS             1857..2174
                     /locus_tag="VNN25_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HXH91773.1"
                     /translation="MSKFKPVMMALSTVLIAGAVFAQDTAATTAPTGVRWGVLAAAFV
                     LGIAAAAGAIGQGRAIAASVEGIARNPGAAGAIRLSMIIGLALIESLVIYALVIAFLA
                     YKL"
     gene            complement(2241..2408)
                     /locus_tag="VNN25_09345"
     CDS             complement(2241..2408)
                     /locus_tag="VNN25_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91774.1"
                     /translation="MNWFNLSFVGWVILIAALSIAAYKLHVPNIWIGIGALALVGIGV
                     IASVAKSKPRI"
BASE COUNT          453 a          809 c          784 g          576 t
ORIGIN      
        1 cgccaacctc ggcatctggg cgaccgtgct catgctgcgt cgccaccaat acgccggcga
       61 gcgtgtcgat gatttcgaag gactcagcaa acgcgccccg ctgtgggcgt ttgcgatggt
      121 catctttctc ctctccctcg gcggcattcc gccgacggca ggcttcatcg gcaagtactt
      181 cctcttcgcc gcggcggtgc aggccggctt cggctggctg gccgtcatcg ccgtcctcat
      241 gtcggccgtg tcgatgttct actacctgcg cctcgtcgtg gcgatgtacc tgcgcgaagg
      301 gcaggaagcg gagttcgcga tcacgacgcc gctcaggatc gtcgccggta tctgcctcgt
      361 cgtgacgctg gcgctcggca tcgtgccgtc gtcattgatc gatcaggccg tgctctcttc
      421 gagccatgtc gccgcccatg cggcgctcac gccccatgcc ggacgataag gacagcgata
      481 gcggaaagaa gatccgccag aacgaaaccc tcctcgaggc gtcgtcgatc ggcctcatgt
      541 tccccatcgc catcggactc ggatatctct gggggtgggg gatggacaag ttcttccaca
      601 ccagtccgtg gctgacgtgg atcttcaccg ggttcggcgt catcgccgca ttcatcaacc
      661 tttttcggat cggcttgcgg aatgacgacg gctcttcagg atgatcctcc cgcggtcgtg
      721 ctcgggcgca tccgccggat cgcgctcgcc gcgtgcggcg tcttcgccat ccccgttctg
      781 gtgcttttgg gatggcgtgg attgatcgga ttgacttgca gtggtctcgt aactatgatc
      841 aatttccttt ggttggagga gatcgtcgag actctgctcc agccagcgca gcatcggcat
      901 ctcgggagag tgacaatccg tacgttgtct cgcttcgctc tcgtcggtgc ggcgcttttg
      961 gtcacaatct tcgtagctcg cgtcaatgct gcaagtgtgt tgctggggtt ctcgattgtg
     1021 gtggtgggaa tcatgggtga agctgtgtac tcgacagtcc gaagcttctc ccgatagagg
     1081 tcgatggaac acgaaagctc aattctgtac gcgccgttca atacgctgtg gcacagcgtg
     1141 cagcaccgct ttgcctggga tgtgccggat cacgtgatca tggccctgct ggtcctgacg
     1201 atcagttgcg tcctgtttcc actgatgtcg cggaggatca gccgcgagaa tcccagccac
     1261 ttccagcagt tgctcgagct ggttgtggga gggctcaggg atttgctgcg tgacatcatc
     1321 gggcacgacg gcgaccgtca cctttacatc atcggcagtt tcgcggtgtt cattttcctg
     1381 tcgaacatct tcgcgctctt cttcttcctt cagccgccga cgagcaatcc gaacacgacg
     1441 tttggcctgg ctctctgcgc cttcgtctac tacaactacc agggcatcaa gacacagggc
     1501 atcgttcact acctgaagca cttcatggga ccgatgctga tcctggcgcc gctgatgatc
     1561 cccatcgaga tcatcggaca tctggcgcgc atcctctcgc tcgggatgcg cttgttcggc
     1621 aacatcttcg gcgagcatgc cgccaccggc atcttcatgg ggatgctgcc cttcgttctg
     1681 ccctggccga tgatgggcct gggcatcttc ggggccttcc tgcagacctt cgtcttcatc
     1741 atgctgacga tggtctacat cggtggagca accgtggtgg aagagcactg atccgcaaaa
     1801 tcgactgtaa cgacatctac agaacgtaac gagatttcag gaaaggagaa aatgaaatga
     1861 gcaagttcaa acccgtcatg atggcgcttt ccacggtgct gatcgccggc gccgtgttcg
     1921 cgcaggatac ggccgcaacg actgccccca cgggcgtgcg ttggggtgtt ctggccgcgg
     1981 cattcgtcct cgggatcgca gcagcagcgg gcgccatcgg tcagggccgc gccattgccg
     2041 cttcggtcga gggcatcgcc cgcaacccgg gcgcggccgg cgcgatccgc ttgtcgatga
     2101 tcatcggtct cgccctcatc gagtcgctgg tcatctacgc gctggtcatc gcgttcctcg
     2161 cgtacaagct gtagcctcga acactctgcg tgcaggcaaa ggcccggtgg gatgacccgc
     2221 cgggcctttg tgtttgcgga tcagatgcgc ggcttcgact tcgccaccga ggcgatgacc
     2281 ccgatgccca ccagcgccag cgcgccgata ccaatccaga tattcggcac atgcagcttg
     2341 taggctgcaa tcgacagcgc ggcgatcaga atgacccagc ccacgaacga aagattgaac
     2401 cagttcacgg agtacctccg ggacgatgct cagcaatggc ggtgccggaa cgctgcggcg
     2461 gcgtctgaaa gcgtcgaatg gactctgcga agactgtcgg cgccgcgctt ggtctctgcg
     2521 acacgttccc gcgcagttga accttgctcg catctctcgg gacagccgag aacaaaaaga
     2581 gccggcgaac atcgccggct cttggcatca tggttcggag gg
//
LOCUS       DATLVW010000303         4243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_139423, whole genome shotgun sequence.
ACCESSION   DATLVW010000303 DATLVW010000000
VERSION     DATLVW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139423"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..714)
                     /locus_tag="VNN25_09350"
     CDS             complement(130..714)
                     /locus_tag="VNN25_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91775.1"
                     /translation="MNIKEETRQQTTAAAADTPPPAATLAPEAVVEQLRTMRAQIGDV
                     APLTAAQRRALRVAGRTSNPIVQSSINVIGALDIVAQAVGQPADEVRQLCDEDNRWTA
                     VEDELRGMLNGVSSSNLIRRQHIALITGRAYNIGTQLARDPAHAVLVPHVEEIKRLKS
                     VKRRKKAAPAPTPKSPASGTSQSEASATPVPAGK"
     gene            1958..>4243
                     /locus_tag="VNN25_09355"
     CDS             1958..>4243
                     /locus_tag="VNN25_09355"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXH91776.1"
                     /translation="MKWLAASLILFAACATTPAPPPPKASVAPAPPQAAVAEKSTIES
                     KTAKLQKFDGFIPLFWDAENGKLFMQLSRFNEELLYQVSLPAGVGSNPIGLDRGLLGQ
                     TQIVTFERIGPKVLMTATNTKFRALSNDEAERRAVADSFARSVIWSFKVEAGDATSAL
                     VDATDFFLSDQQGVTRSLRNTQQGNYSLDKNRSAIYLPRTKVFPRNTEVEAVLTFQTG
                     ERPGNFVSGVTPIADSLTVREHHSLVALPPPGYTPRRADPRVGVFAIDFFDYASPFTG
                     PIEKQWIARHRLIKKDPSAAVSEPVEPIVYYVDAGAPEPIRSALLEGVSWWSAAFEAA
                     GFKNAFQVKVLPPDADPMDLRYNMINWVHRAQRGWSYGAAVIDPRTGEIIKGNVTLGS
                     LRIRQDVMIAEGLIPQFDELHDKALADLDPMTSPSVMALARIRQLAAHETGHTLGLDH
                     NMAASSYGRASVMDYPSPYVKITDGKLDLSDAYTKSIGSYDKWVIRYAYAQFAPDANE
                     EQELDRIVREAPLFITDPESRPVSAAHPDASVWDSPGNNVGMLRHEIEVRRIALSQFG
                     LRNIPMGTPLAQLEEMLVPLYLHHRYQLEAAAKSIGGLDYRYSVKEEGGLVPTPTRRI
                     VPAAAQREALNVVMSTLDPAFLEIPPRILALIPPRGNVDVTSNTELFEHRTTPAFDPV
                     SAAMASADITLAALLDARRAARLVQFHAEDAANPDFNEVVNDVIATVTRRESGYRGAI
                     TRASARLAATRLMNLAANADAD"
BASE COUNT          753 a         1355 c         1311 g          824 t
ORIGIN      
        1 ctcgagatct ttgtctctca tggccgttct ctcctatcgc agcgttcgcg gaggaaaacg
       61 accgggcgcg tttgcacgcg cccggtctca cttcctcctt tcacatagac gattgagaac
      121 tgccggaagt tacttacccg ccggtaccgg ggttgcggat gcttctgact gcgatgtgcc
      181 ggatgccgga gacttcggcg tgggagccgg cgctgccttc ttgcggcgct tgacgctctt
      241 cagacgcttg atctcctcta catgcggcac gagaacggcg tgggccggat cgcgcgccaa
      301 ctgtgtaccg atgttgtacg cacgcccggt aatcagtgcg atgtgctggc ggcggatgag
      361 gttcgaactg gacacgccgt tcagcatgcc ccggagctca tcctcgaccg ccgtccagcg
      421 gttgtcctcg tcgcacaact gacgcacctc gtcggccggc tggccgaccg cctgcgccac
      481 gatgtccagc gcaccgatga cgttgatcga cgactgcaca atgggattgg acgttcttcc
      541 ggctacgcgc agtgctctgc gctgcgcggc cgtcagcggc gccacatcgc cgatctgcgc
      601 tcgcatcgta cgtagttgtt cgacgactgc ctcgggtgcc aatgtcgctg ccgggggcgg
      661 agtgtccgcc gcggcggcag tggtttgctg cctggtttct tccttgatat tcatatcgtt
      721 ctcctaaaga tcacgggttt gttaaggccg gagggaacga cgagcacttg tgcacgagtg
      781 caggcggcca ccggctggaa gagaacggcc atgagccggc tcttccggtt gatgtttccc
      841 cgaagtgcgc gcacttcgag agcccgcggc aggaggagca ccgctcaact cccgcagaga
      901 tgagaagcag tcgcactttc cagcgccgtc gttcccaccc gttggccgcc aggagcgtgg
      961 ggctctcacg agcgccaact ccgtcctcgt tgagccggct gaccagctta acgaggccgt
     1021 ggacgataac atgagcttgg tggggatgtc aaggggtgtt tttgggtttt tctttcactt
     1081 cttttctcac tctttctcaa cgctctcaaa tcgagcgccc ttctccccca cgcttttcag
     1141 tgggggagaa ggtggccgaa ggccggatga gggggcgttc gactgttgcg cagcgtcccc
     1201 aggcatcagg aagaacacag ggcaggtctc tcgcggagaa catgcttgtc ccatcttgca
     1261 acgcccccct catccgcctt cggcaccttc tccccccgca aaaagcgcgg ggggagaagg
     1321 cgctcgattg gaaagcactc caatgaaaag gaagaggtga ggaggtgcac gaagcctatg
     1381 agaaatgcag gctcactccc gtgcgcacgc aatcgacgag tttccgcgtg cggattggag
     1441 ggtttaccgt gcggacctga tggttttcca tgcgcaccct tgggtattca atgcggaccc
     1501 ggaggtttac cgtgcgaacc cgagggttta ccgtgcaaac ctgagggttt tccatgcaaa
     1561 cctgagggtt ttccatgcgc acccttgggt attcagtgcg aacccggagg gtttaccgtg
     1621 caaccctgag ggtttaccgt gcaacctcga gggtttaccg tgcaaccctg agggtttacc
     1681 gtgcaaccct gagggtttac cgtgcaaccc tgagggttta ccgtgcaacc ccgagggttt
     1741 agcgtgcaac cccgagggtt ttaccgtgca accccgaggg tttaccgtgc aaccccgagg
     1801 gtttaccgtg taaccctgag ggtttaccgt gcgacccgga gggtttcccg cgcggaccgg
     1861 acgattctcc gcccaccctg ccgattcccc gcgcgtacca cctggcgcgc agcttcgcgc
     1921 actgtggtca caagcaatac ccggctagaa tcgcggcatg aaatggcttg ctgcttcgct
     1981 cattctgttc gctgcctgtg cgaccacgcc ggcgccgcct ccacccaaag ccagcgtcgc
     2041 gccggctccg ccgcaggcgg ccgttgcgga gaagtccacg atcgaatcga agacggcgaa
     2101 gctgcagaag ttcgatgggt tcattccgct cttctgggat gctgagaacg gcaagctgtt
     2161 catgcagctt tcgcggttca acgaagagct gctttaccag gtctcgctgc ctgctggtgt
     2221 cggatcgaat ccgatcgggc ttgaccgcgg gctgctcggg cagacgcaga tcgtcacctt
     2281 cgagcgcatc gggccgaaag tgttgatgac agcgacgaat actaagtttc gtgcgctgag
     2341 caacgatgag gcggagcggc gcgccgtggc cgactcgttt gcgcggtcgg tgatctggtc
     2401 gttcaaagtc gaggcgggcg atgcgacgtc ggccctcgtc gacgccaccg acttttttct
     2461 cagcgatcag cagggtgtga cgcgctccct tcgcaacacg cagcagggca actactcgct
     2521 cgataagaac cgcagcgcga tttatctgcc gcgcacgaaa gtctttccgc gcaacaccga
     2581 ggtcgaggcg gtgctgacgt ttcagacggg ggagaggccg gggaacttcg tcagcggcgt
     2641 cacgccgatc gccgacagcc tcaccgttcg cgagcatcat tcgctcgtgg cgctgccgcc
     2701 gcccggttac acgccgcggc gcgccgatcc gcgcgtcggc gtcttcgcca tcgacttctt
     2761 cgattacgcc agtcccttca ccgggccgat cgagaagcag tggatcgcac ggcatcgttt
     2821 gatcaagaaa gatccgagcg ccgcggtctc agagccggtc gagccgatcg tttactacgt
     2881 cgacgcaggc gcgcccgagc cgatccgcag tgcgctgctg gaaggcgttt cgtggtggag
     2941 cgcggccttc gaagccgccg gtttcaagaa tgcgtttcag gtgaaggtgc tcccgcccga
     3001 cgccgacccg atggaccttc gctacaacat gatcaactgg gtgcatcgcg cgcaacgcgg
     3061 ctggtcgtat ggcgcggcgg tcatcgatcc tcgcaccggc gagatcatca aaggcaacgt
     3121 caccctcggg tcgctgcgca tcaggcagga cgtcatgatc gccgaggggt tgattccgca
     3181 gttcgatgag ctgcacgaca aggcgctcgc cgatctcgat ccgatgacgt cgccgtccgt
     3241 gatggcgctt gcgcgcatcc gccagctcgc cgcgcacgag accggccaca cgctcggcct
     3301 cgatcacaac atggccgcct ccagctacgg ccgcgcctcg gtgatggact atccgtcgcc
     3361 gtacgtaaag atcactgacg gaaagctcga cctgtccgat gcctacacga aaagcatcgg
     3421 ctcgtacgac aagtgggtga tccgttacgc ctacgcgcag tttgcgccgg atgcgaacga
     3481 agagcaggag ctcgaccgta tcgtccgtga ggcgccgctc ttcatcaccg atcccgagtc
     3541 acgtccggtc agcgccgcgc atcccgatgc ctcggtgtgg gattcgccgg gaaacaacgt
     3601 tggcatgctc cgtcacgaga tcgaagtgcg ccgcatcgcc ctctcgcaat tcggactgcg
     3661 caacattccg atggggacgc cgctggcgca gctcgaagag atgctcgtgc cgctctatct
     3721 gcatcatcgc tatcagctcg aggctgcggc gaagtcgatc ggcggactcg attaccgcta
     3781 cagcgtgaaa gaggaggggg gactggtacc gacgccgacc cggcgcatcg ttcccgccgc
     3841 ggcgcaacgc gaggcgctca acgtcgtgat gtcgacgctc gatcctgcgt ttctcgagat
     3901 tccgccgcgc atcctcgcgc tcatcccgcc ccgcgggaat gtcgatgtca ccagcaacac
     3961 ggagctgttc gagcatcgca cgacgccggc gttcgatccg gtgagcgcgg cgatggcgtc
     4021 ggccgacatc acgctcgccg cgctgctcga tgcccgccgc gccgcgcggc tcgtgcaatt
     4081 tcacgccgag gatgccgcaa atcccgattt caacgaggtc gtcaacgatg tcatcgcaac
     4141 agttacgaga cgcgagagcg gctaccgcgg ggcgatcacc cgcgcctcgg cacgcctggc
     4201 agcgacgcga ctgatgaacc tcgccgcgaa cgccgacgcc gat
//
LOCUS       DATLVW010000304         7844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_422935, whole genome shotgun sequence.
ACCESSION   DATLVW010000304 DATLVW010000000
VERSION     DATLVW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422935"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VNN25_09360"
     CDS             <1..463
                     /locus_tag="VNN25_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HXH91777.1"
                     /translation="ILILVVIALAAVSYWALKHRRESRLANEERLSTEAKTVVTSPPP
                     KAPAALPQTSTAPVAPEPGQPARLLLSIEVSEPSVVVLEADGETVINDELRRGYHRTI
                     EAKERFRFRTIGNAAGIVLTLNDKPVPPLGRGGQVLHDVVFDRESLQKTQQ"
     gene            463..1554
                     /locus_tag="VNN25_09365"
     CDS             463..1554
                     /locus_tag="VNN25_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91778.1"
                     /translation="MSASSSELSGDIIIERITSGEYPREAVETFARGFLPLPQDDLIV
                     VLAYLTRSPDADIASAARSSLADVPSRSIHSFAANESLPAAHLSMLLRASEDPFVLEA
                     LIRNRAVPDELIVELAAVADAAVQEVVAINQARIIRTPAILDALLANPQLTPDVRRRA
                     LETREEFFDKKARIAAQQPAEVDEDEAMLTLSDEPIADLLEKAAQEPESDSPPPALME
                     SEKLDEKKLSVFSQILVMTVSEKVQMGFKGGKTERMILIRDHNRLVCGAVMRNPRMSE
                     SEVESIASMRNIAEEVLRLITLKREWISKYNIAVTLARNPKCPVGVVLPLINRLTLRD
                     LKGLKDDKGVSETVRGLARKVYIQRSQKS"
     gene            1555..2217
                     /locus_tag="VNN25_09370"
     CDS             1555..2217
                     /locus_tag="VNN25_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXH91779.1"
                     /translation="MKVNYYEVLGVDRSASEPEIRERFRKLAREQHPDRYKGSDKADA
                     ERKFQTLTEAVNVLTNTARRKQHDAEIGSAGSRATTGPADFVQVSKVYLSRGVKAYKE
                     GDMSAAYENFDMAAKHNPADGKAFHYLALAATRIPAYSRQAVQAIETAVQREPINPLF
                     LRDAGSICKRAGLTAKAERYLEEALKWDTDNPEIRAALAELRQRGEAKEGGKGFTLFK
                     KG"
     gene            2221..2979
                     /locus_tag="VNN25_09375"
     CDS             2221..2979
                     /locus_tag="VNN25_09375"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXH91780.1"
                     /translation="MDVKAYGLTHVGRQRQHNEDSFLVADEAKLFLVADGMGGHAAGE
                     IASRIAVDSISEFIVHTKEDDGTWPHAYDEHYSRTTNRLMAALRMANTRVLEAMRKDA
                     RLRGMGTTVVAAMADGDKMSVAHVGDSRAYMIRNGKITRITSDHSWVFEQVQAGMLTE
                     AEAEKHPLRNVITRALGGALSVNPDASEIESRPGDFYLLCSDGLTGMVPEDEILKLVT
                     GNADDLEKACKELIDTANERGGLDNVTAILVKTI"
     gene            3054..3281
                     /locus_tag="VNN25_09380"
     CDS             3054..3281
                     /locus_tag="VNN25_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3084..3086,aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HXH91781.1"
                     /translation="MHIRIDYCGVUNYLPRASSLAAEIKREFGIDSEVVRGSGGIFVV
                     SIDGKIIFSKKEEGRFPTEREILDKVTALRA"
     gene            3496..3783
                     /gene="gatC"
                     /locus_tag="VNN25_09385"
     CDS             3496..3783
                     /gene="gatC"
                     /locus_tag="VNN25_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXH91782.1"
                     /translation="MKIDRDEAKRIAGLAHLEFDDAGLDRMAGEMTKILSYIDQLREV
                     DVAGFDGGSSAEATPLRDDVPRPSVDRAAVASNAPAFSADGHFIVPKVIGE"
     gene            3776..5167
                     /gene="gatA"
                     /locus_tag="VNN25_09390"
     CDS             3776..5167
                     /gene="gatA"
                     /locus_tag="VNN25_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXH91783.1"
                     /translation="MSELLNLPWHEAKTQATARELVDASLRAIDERNPALNAFLHVAR
                     DAADGMPVAVKDNIVTTGMPTTCASRILGSFVSPFDATAVARLRQSGCSIIGKTNLDE
                     FAMGSSTEHSAFGTSRNPWDTTRVPGGSSGGSAVAVAARMVPAALGSETGGSVRQPAA
                     LCGVVGLKPTYGRISRNGLVAFGSSVDQIGIVTKTVRECAELLGVIAGHDPLDSTSSS
                     ALVDDYAADLDAGIRGLRFGVVREAVSQLEGEVRANFDDALAVLRGAGAVIEEVSVPT
                     IGYAIAVYYIVANAEASANLSRFDGIRYGHRSARAKTIREVYFESRGEGFGDEVKRRI
                     MLGTFALSSGYYDAYYGRAQAVRARLRADFDAAFARFDYLLTPTTPEPAFAIGAKTGD
                     PLAMYLSDIFTAPANLVGIPAIAVPSGFSRGKLPLSLQVMGPHFSERALFRAASAFER
                     ETGYWRELPPACK"
     gene            complement(5294..5701)
                     /locus_tag="VNN25_09395"
     CDS             complement(5294..5701)
                     /locus_tag="VNN25_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91784.1"
                     /translation="MKQEPERRFRGRKKYLLRRMNGGVRIKPELIRIKPGLIGRKEEM
                     IRINPKMIPIKNKMIPIPPKMIQIQKKDDPDLSQDDPDQRKDDPDLSQDDPDPRKDDP
                     DLSQDDPDPRRDDPYQFKDDPDPRKRRSGSIQR"
     gene            complement(5720..6787)
                     /gene="cheB"
                     /locus_tag="VNN25_09400"
     CDS             complement(5720..6787)
                     /gene="cheB"
                     /locus_tag="VNN25_09400"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HXH91785.1"
                     /translation="MPLRALVIDDSAYNRVTISRMLESSPKIKVVATAVNGEDGIKQV
                     MKHKPDVITLDLEMPVMDGFAFLRWLMANIPTAVIAVSSRSSDRSVFKALDLGAIDFI
                     GKPGGRVSPRLEEIERDLVAKVLQVADIRMENLRRRIVEEEQLEPQDTGPSGSCEGGI
                     ELVAIGCSTGGPPALQHVFQSLPLLPIPIVVAQHMPPAFTRLFAERVNKLTSYNVKEA
                     DDGEKIENGTVYIAPGGMQMEVRRNADGLLLRTFPAKVADVYAPSVDRLLISASDACG
                     ERLVAIIMTGMGDDGAQAIRRVRERGGKTIAESAQSAIIFGMPNEAIKTGCVDQVLPL
                     GEIPRAIHRLCSGGDSNLRAV"
     gene            complement(6812..7663)
                     /locus_tag="VNN25_09405"
     CDS             complement(6812..7663)
                     /locus_tag="VNN25_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXH91786.1"
                     /translation="MSTFNLSLNHHLDLPDDVFRLMRDQIYKRTGMWFSDNSKYLLQK
                     RLSPRAKELNFDSFQKYFYFLQYDPRAEAEFDQIYDLVTTNETYFFREPAQLAAFAEE
                     IVPEILSRKTVKKIRIWSAGCSSGEEPYSIAMLLSEAGHYDHAVFEIFASDINQQVLA
                     KARRGFYRESAFRATEIPLRDKYFSPNGDGSWRLHDDIRNRVSFGRLNLYDEARVSLL
                     GHLDIIFCRNVIIYFDDVSKKVVVTNFYNRLSESGYLLLGHSESLISLSTQFKLRHLK
                     NDMVYQK"
BASE COUNT         1511 a         2370 c         2426 g         1537 t
ORIGIN      
        1 catcctcatc ctcgttgtga tcgcactcgc cgccgtcagc tactgggcgc tgaaacatcg
       61 acgcgagagc cgcctcgcca atgaggaacg tctgagcacc gaggccaaaa cggtcgttac
      121 ttcgccgccg ccgaaagcgc ccgcggcgct gccgcagacc tcgacggctc ccgttgctcc
      181 tgagccgggg cagccggcgc gtttgctact gagcatcgag gtcagcgagc catcggtcgt
      241 ggtcctcgaa gccgacggcg agaccgtcat caacgatgaa cttcggcggg gctaccaccg
      301 cacgatcgaa gcgaaggagc gtttccgctt ccgcacgatt ggtaacgcgg cagggatcgt
      361 attgacgctc aacgacaagc cggtgccacc gctcgggcgg ggagggcagg ttctgcacga
      421 cgtcgtgttc gatcgcgaat ccctccaaaa gacccagcaa tgatgagcgc ttcctcctcc
      481 gagctgtccg gcgacatcat catcgagcgg atcacttccg gcgagtaccc gcgtgaagcg
      541 gtggagacgt tcgcgcgcgg ttttctgcca ctgccgcagg acgatctcat cgtcgttctg
      601 gcgtatctga cacgatctcc cgatgcggac atcgcatccg cggcgcgcag ctcgctcgcc
      661 gacgtcccct cgcgcagcat tcattcgttt gccgcgaacg agtctctgcc ggcggcgcat
      721 ctctcgatgc tgcttcgggc gagcgaggat cccttcgtac tcgaggcgtt gatccgcaac
      781 cgggccgtgc cggacgagct gatcgtggag ttggccgccg ttgctgatgc cgccgtgcag
      841 gaagtcgttg ccatcaatca ggctcgcatc attcgcactc ccgcgatcct tgacgcactc
      901 ctcgcgaatc cgcagctcac cccggacgta cgccgccgtg ccctcgagac gcgcgaggag
      961 ttcttcgaca agaaagcgcg catcgccgca cagcagccgg ccgaggtcga tgaggacgag
     1021 gcgatgctca cgctctccga cgagccgatc gccgatcttc tggagaaagc ggcgcaggag
     1081 ccggaaagcg attcgccgcc gcccgcgctg atggagagcg agaagctgga cgagaagaag
     1141 ctctccgtct tttcccagat tctggtgatg actgtttccg agaaagtgca aatggggttc
     1201 aaaggcggca aaaccgagcg gatgatcctc atccgtgacc acaaccggct ggtgtgcggc
     1261 gctgtgatgc gtaatccgcg tatgagcgag tcggaggtgg agtcgatcgc ctcgatgaga
     1321 aacatcgcgg aagaagtgct ccggctcatc accctgaagc gcgagtggat ctccaagtac
     1381 aacatcgccg ttaccctggc gcgaaatccg aagtgcccgg tgggggttgt cctgccattg
     1441 atcaatcgat taaccttgcg tgatttgaag ggcttgaagg acgacaaagg tgtgagcgaa
     1501 accgtcagag gcctggcgcg gaaggtctac atccaacgca gtcaaaaatc ctgaatgaag
     1561 gtcaactact acgaagtgct cggagtcgat cgctccgctt ccgagccgga aatccgggag
     1621 cgtttccgga agcttgcccg cgagcagcat cccgaccgct acaaaggctc cgacaaagcc
     1681 gatgccgagc ggaagttcca gaccctgacc gaagccgtca acgtgctgac gaatactgcc
     1741 cgtcgcaagc agcacgacgc tgagatcggt tcggcgggat cgcgcgcgac gaccggtcct
     1801 gctgatttcg tgcaagtgtc caaggtttac ctatcgaggg gagtcaaggc gtacaaggaa
     1861 ggtgacatga gcgccgcgta cgagaacttc gacatggccg cgaagcacaa tccggcggat
     1921 ggcaaagcct ttcactatct cgcgctcgca gccacccgca ttccggcata ttctcgtcag
     1981 gcggtgcagg caatcgagac ggcggtgcag cgggaaccga tcaatcccct gttcctgcgg
     2041 gacgccggat cgatctgcaa acgcgccggg ctgaccgcaa aagcagagag atatctcgag
     2101 gaagcgttaa agtgggacac agacaatcct gaaatccgtg cggcactggc cgaattgcgt
     2161 cagcgtggtg aggcaaagga aggcggaaag gggtttaccc ttttcaaaaa gggctgagga
     2221 atggacgtga aggcatacgg cctcacacat gtcggacggc aacggcagca caacgaggat
     2281 tcgttcctcg tcgctgatga ggcgaagctg ttccttgtcg ccgacgggat gggaggacat
     2341 gccgctggtg agatcgcctc ccggatcgcc gtcgactcga tctccgagtt catcgtccac
     2401 acgaaggaag acgacggcac gtggccgcac gcctacgacg agcactactc gcggacgacg
     2461 aaccggctga tggcagctct gcgcatggcc aatacccgcg tcctcgaagc gatgcgcaag
     2521 gacgcccgcc tgcgcggcat gggcacgacc gtggttgccg ccatggcgga cggcgacaag
     2581 atgtcggtag cgcacgtggg cgattcgcgc gcctacatga tccgcaacgg taagatcacc
     2641 cgcatcacga gcgatcactc ctgggtcttc gagcaggtgc aggcgggaat gctcaccgag
     2701 gcggaggcgg aaaagcatcc tcttcgcaac gtgatcaccc gcgccctcgg gggcgccctc
     2761 tccgtcaatc ccgatgcctc cgaaatcgaa agccggcccg gcgatttcta tctcctctgc
     2821 tccgacggcc tcactggcat ggttcccgaa gatgagatcc tcaagctcgt gaccggcaac
     2881 gccgacgatc tcgaaaaggc ctgcaaggag ctgatcgata ccgccaacga acgaggcgga
     2941 ctggataacg tgaccgcgat cctggtgaag acgatatagc agagatcagg aaccagagat
     3001 cagagacaaa aggccctgat ctctgatctc tagtctctgc tctctcaaac ccaatgcaca
     3061 ttcgaatcga ctactgcggc gtttgaaact acctcccacg agcctccagt ctggcggctg
     3121 agatcaaacg ggaattcggg atcgacagcg aagtcgtccg cggcagcgga ggaatcttcg
     3181 tcgtctcgat cgacggcaaa atcatcttct cgaagaaaga ggaaggccgc ttcccgaccg
     3241 agcgcgagat tctcgacaaa gtgaccgcgc tgcgcgcctg acgtagtgca ggcattcttg
     3301 cctgctcccg ccgggcattc ctgcccggcg gtgtactcga gccgagattc gccaatctgt
     3361 cagttttcga gaggtgagaa ccccgtgcat gcccatcgat attgatttga gcttcgaaac
     3421 caccgccggg cagggacgcc cggcgggagc aggcaggaat gcctgcgcta ctcgtcgcga
     3481 tacacttcgt ggccgatgaa gatcgatcgc gacgaagcaa aacgcatcgc cggcctggcg
     3541 catctcgaat tcgatgacgc cggtctggac cggatggccg gcgagatgac gaagatcctt
     3601 tcgtacatcg accaactccg cgaggtggac gtcgccgggt tcgacggcgg ttcgtccgcc
     3661 gaggccacac cgcttcgcga cgatgtgccg cggccgtccg ttgatcgtgc ggcggtagcg
     3721 tcgaatgctc ccgcgttcag cgctgacggg cacttcatcg tcccgaaggt gatcggtgag
     3781 tgagctcctt aaccttccgt ggcatgaagc gaagactcag gccaccgcgc gtgagcttgt
     3841 cgacgcctcg ctccgcgcca ttgacgagcg caaccctgcg ctgaacgcgt ttctgcacgt
     3901 tgcccgtgat gccgcggacg gaatgccggt tgcggtgaag gacaacatcg tcaccacagg
     3961 aatgccgacg acgtgtgcgt cgcggattct cgggtcgttc gtctcgccgt tcgatgcgac
     4021 ggctgtggcg cggttgcgcc agtccggctg cagcatcatc ggcaaaacca atctcgacga
     4081 gttcgcgatg ggatcgtcga ccgagcattc cgcgttcggc acctccagga atccgtggga
     4141 tacgacccgc gtgccgggcg gatcgtcggg tggctcggcg gtcgccgtgg ccgcgcggat
     4201 ggtgcccgcc gctctcggct ccgagaccgg cggctccgtc cgtcagcctg ctgcgctctg
     4261 cggcgtggtc ggactgaagc cgacctacgg ccgcatcagc cggaacggcc tcgtggcgtt
     4321 cggatcgagc gtcgatcaga tcgggatcgt gaccaagact gtccgcgaat gcgccgagct
     4381 cctcggcgtc atcgccggcc acgatccgct cgattcaacc tcgtcatccg cgcttgtcga
     4441 tgactacgcc gcggatctgg atgccggcat ccgagggctc cgcttcggcg tcgttcggga
     4501 ggccgtctcg caactggaag gggaggtgcg cgccaatttc gacgacgctc ttgccgtact
     4561 tcgcggcgcc ggcgcggtca tcgaagaggt cagcgttccg accatcggct acgccatcgc
     4621 cgtctactac atcgttgcga acgccgaggc gagcgcgaat ctctcacggt tcgacggcat
     4681 tcgctacggg catcgcagcg cgcgcgcgaa aacgattcgc gaggtttact tcgagtcgcg
     4741 cggcgaaggt ttcggcgacg aggtcaagcg ccggatcatg cttggcacct tcgccctttc
     4801 gtccggctat tacgatgcct actacggccg cgcccaggcc gtgcgggcga ggctgcgcgc
     4861 cgatttcgac gccgcgtttg cccgcttcga ttaccttttg acgccgacga ctcccgagcc
     4921 ggcattcgcg atcggcgcaa agaccggcga tccgctggcg atgtacctga gcgacatttt
     4981 cacggctcct gcgaacctcg tcggcatccc agccatcgcc gtcccctccg gtttttcgcg
     5041 cgggaagctg ccgctgtcgc ttcaggtcat ggggccgcat ttttccgagc gcgccctctt
     5101 ccgcgcggcc agcgcgttcg agcgcgagac cggatattgg cgcgaattgc cgccggcgtg
     5161 caaatgacga ttggctgtca gccagcgatg ccagcgccaa tcttatcaac gagttacaac
     5221 ttccaggaaa gcaacggcct gatcctctgc cggccggaac ggccgattcc gatcatctct
     5281 tgtttgatcc ggatcatctt tggattgatc cggatcgtct ttttcttgga tccggatcat
     5341 ccttgaattg atagggatca tctcttcttg gatctggatc atcttgggag agatcgggat
     5401 catcttttct tggatccgga tcatcttggg agagatcggg atcatctttt ctttgatccg
     5461 gatcatcttg ggagagatcg ggatcatctt ttttttggat ctggatcatc tttggaggga
     5521 tcgggatcat cttgtttttg atcgggatca tctttggatt gatccggatc atctcttctt
     5581 tcctcccgat caaccctggt ttgatccgga tcaactctgg tttgatccga acccctccat
     5641 tcatcctcct gagcagatat tttttcctgc cgcggaacct tctttcgggc tcttgcttca
     5701 aaagtatgtc tgagagagat cagacggccc gcaggttaga gtcgccaccg ctgcacagac
     5761 ggtgaatcgc ccgcgggatc tcgccgagcg ggagaacttg atcgacgcag ccggtcttga
     5821 tcgcttcgtt cggcatgccg aagatgatgg cgctttgggc ggattcggcg atcgtcttgc
     5881 cgcctcgctc gcggacgcgg cggatggctt gcgcgccgtc gtcgcccatg ccggtcatga
     5941 tgatggccac gaggcgttcg ccgcaggcgt cgctggcgga gatgaggagc cggtcgacgg
     6001 atggcgcgta gacgtcggcg acctttgcgg gaaacgtccg caagagcagc ccgtccgcgt
     6061 tccggcgaac ttccatctgc atgccgccgg gtgcgatgta gaccgttccg ttctcgatct
     6121 tctcgccgtc gtcggcctct ttcacgttgt agctggtgag cttgttgacg cgctcggcga
     6181 agagccgggt gaacgcgggc ggcatgtgct gcgcgacgac gatcggaatc ggcagcagcg
     6241 gcagcgattg aaaaacatgc tgcagcgccg gtgggccgcc cgtggagcag ccaatggcga
     6301 cgagctcgat cccgccttcg cacgagcctg acggacctgt gtcctgcggc tcgagctgtt
     6361 cctcttcgac gatccggcgg cgcagattct ccatgcggat gtcggccacc tgcagcacct
     6421 tggcgacgag atcgcgctcg atttcctcga gccgaggcga aacacgtcca cccggcttgc
     6481 cgatgaagtc gatcgcgccg aggtcgaggg ctttgaagac actgcggtcg ctcgatcgcg
     6541 acgacacggc aatgaccgcc gtcgggatgt tcgccatcag ccagcgcaga aatgcgaatc
     6601 cgtccatgac cggcatctcg agatcgagcg tgatcacgtc aggcttgtgc ttcatcacct
     6661 gcttgatccc gtcctcaccg ttgacggcgg tggccacgac cttgatcttc ggcgacgact
     6721 ccagcatccg cgagatcgtc acgcggttgt aggcggaatc gtcgatgacg agggcacgaa
     6781 ggggcattcg ggcgggcatc ctaccagcgc gttacttctg gtaaaccatg tcgtttttca
     6841 ggtggcgcag tttgaattgc gtggagaggg aaatgagcga ctcgctgtgg ccgaggagga
     6901 gatagccgga ctcggagagg cggttgtaga agttcgtgac gaccaccttc ttcgagacat
     6961 cgtcgaaata gatgatgacg ttgcggcaga agatgatgtc gaggtgtccg aggagtgaga
     7021 cgcgcgcctc gtcgtaaagg ttgaggcggc cgaaggaaac gcggttgcgg atgtcgtcgt
     7081 gcaggcgcca cgatccgtcg ccgttgggac tgaagtactt gtcgcgcagc gggatctccg
     7141 tggcccggaa cgcgctctcg cgatagaacc cgcgccgcgc cttggccagg acctgttggt
     7201 tgatgtcgct ggcgaagatc tcgaagacgg catggtcgta gtggcccgct tcggacagca
     7261 gcatggcgat cgagtacggt tcttcgcctg acgaacaacc cgcggaccag atgcggatct
     7321 tcttcaccgt cttgcggctc aggatctcgg ggacgatctc ctcggcgaag gcagcgagct
     7381 gcgcgggctc gcgaaagaaa taggtctcat tcgtcgtgac gaggtcgtag atctgatcga
     7441 actcggcttc cgcccgcggg tcgtactgca ggaagtagaa gtacttctgg aacgagtcga
     7501 aattcagttc cttggcgcga ggcgagagcc gcttctgcag caggtacttc gaattgtcgg
     7561 agaaccacat tcccgtccgc ttgtaaatct gatcccgcat cagcctgaac acgtcgtccg
     7621 gcagatcgag atggtgattc agggacagat tgaatgtgga catgggacac cagagatcag
     7681 ggactagaga tcagagatca ggaaggcatg gccttctctc tgatatctgg tcagtagtct
     7741 ctgatttctg cttctctctc gcttcaaaca acagggatca gagatcaaaa cgaaggcccg
     7801 gcgacggatc ccctgatctc tgatctctag tctctgatct ctgc
//
LOCUS       DATLVW010000305         9169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_261219, whole genome shotgun sequence.
ACCESSION   DATLVW010000305 DATLVW010000000
VERSION     DATLVW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261219"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VNN25_09410"
     CDS             complement(<1..557)
                     /locus_tag="VNN25_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH91787.1"
                     /translation="MRFNRFLALLAVLLSLVLPSAAFAKEKVIVLGFDGVDARYTEQW
                     MNEGKLPNLARLRTLGTFRPLRPTVPAQTPVSWSTFSTGIDPGRTGIFDFLRRDPKTY
                     LPVFAAFDETKEPFLLGDKNPIAAAAIVLVFLALIALLFRRTARYVVLALAVIAGVGA
                     FVATKQYIPVSRPGVINRRQGIPFW"
     gene            607..2115
                     /locus_tag="VNN25_09415"
     CDS             607..2115
                     /locus_tag="VNN25_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH91788.1"
                     /translation="MRTRQLFASLAVVLLAACSGGEKNGSAGDQKTLVIALKSGPTNL
                     DSRVGADNASSRMFDLVYSGLIKVTPDFNYAPDLATKWETPDDKTIVFHLNPNAKFHN
                     GQPVKAADVKWTYESLMNPTFVTSKRSGYSTVDHIETPDEQTVIFKLKEANAGIFDNL
                     TVGILPTGADTNVYKTRPIGCGPYKLVEHRPDDRLEFEAFDQWHGGAPKIKHITVRII
                     PDATTCILEMRRGTVNFEVNIIPFENVAEFEKNPDFKVVKSNGSVYQYLAFNMNDPAL
                     AKPLVRQAIAHAIDRDRIIRDIQRGYAQKTESMLAEGHWARAANLPSYDYDPVKAKQL
                     LAQAGYPNGLNLVFKTSTDAEANSRAQVIQDMMKKAGFNVSIQSNEMSTFFADIGKGN
                     FQMYSLSRNGISDPDFYYVIFYSKNTPPNGQNRGSYANPRVDQLLVEGHSTFDRAKRK
                     PVYDEIQRIIATDLPYISLYMQSNVAIMRKNIDGYVQYPAGFYTSIPQMTMK"
     gene            complement(2189..2659)
                     /locus_tag="VNN25_09420"
     CDS             complement(2189..2659)
                     /locus_tag="VNN25_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH91789.1"
                     /translation="MDIQIDDLSRADIVALIREHLRSAALHSPPESIHALDLDGLRDP
                     AITFWSARQDGELLGCCALKELSKTHGEIKSMRTAAAHQRKGVASRLLEQILDEAKRR
                     GYERVSLETGTMEAFAPARELYRRFGFEPCPPFAQYFEDPYSVCMTKVWVRSKV"
     gene            complement(2662..3171)
                     /locus_tag="VNN25_09425"
     CDS             complement(2662..3171)
                     /locus_tag="VNN25_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91790.1"
                     /translation="MADHPLTLITCAYFVELVIVAYLTRATSRRILGAVVGGVAVAFV
                     TMGIVAICVWAGWWLMPIASTPFLATMLGIGVAISCSPIYLVTWRIARRFGLKGLVIS
                     TAIVTIIGPPRDYFIAAKFPEWMVFLPGVAPIVADAVAYAVIMIVGHVVMYLVAGPSG
                     ADGLARTAK"
     gene            complement(3242..3979)
                     /locus_tag="VNN25_09430"
     CDS             complement(3242..3979)
                     /locus_tag="VNN25_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HXH91791.1"
                     /translation="MEHTPFLTAAWHNLAMINYEVDPKVLQPYVPAGTTLDAWQGRYF
                     ASLVGFQFLHTRVLGIPIPFHRNFEEVNLRFYVRREVGEEVRRGVVFIRELVPRFAIA
                     LVARAMYNEPYLALPMRHTISLEPALTASYSWRLGGRWHRFAVAADRPAALPAAGSLE
                     EFITEHYWGYTRQRDGSTIEYQVTHPQWPVSGGVADIDADFAVLYGPEIAGHLQTVAS
                     CFIAQGSLVSVLPGSGLKSKVPKTIVP"
     gene            4102..5490
                     /locus_tag="VNN25_09435"
     CDS             4102..5490
                     /locus_tag="VNN25_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXH91792.1"
                     /translation="MQKPAQQFDRSLVEGPIVPAVWRLAWPTMLQNIIAGVQGIIDHV
                     MVGHYVGYTGNAAIGVSWQVILVVIVFISSLFTGMAVLVARFAGANEPEKVNRVVYQA
                     FLTALLMSAIMGVIGYFAAPALLDIIKAAPEVQHQALPFLRAMFVGIFGMMLFFMLSG
                     AFRAAGDAKTPLRLGAAITVLTIVFNVILIPRLGTIGAAYGTIASSTLVSAYGVWRLF
                     APHSVIRFRKGMDLSPDFSIIRSLFRFGLPTGVQGIAMNIGGVLMLRFIGSLQHSAAA
                     QAAYAVCYTELFSLITWTSVGLLGASVTIAGQNLGANRPDRTRLGVAVASRIGLGVAA
                     VIGLMFLLIPNVLLGVFGMKDPLVTSIGTQLLRYLSVSGFFITVALSYTGGLQGTGDT
                     RSPLYISIVSQIIIPIGICAIIQTMRPLQPADIWRAIVLGHLTRAILSVLRFRQGKWQ
                     NIEVDLERAPAN"
     gene            complement(5616..6560)
                     /locus_tag="VNN25_09440"
     CDS             complement(5616..6560)
                     /locus_tag="VNN25_09440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXH91793.1"
                     /translation="MSLDPAQFEEPINQLRRQIEEATAAERDPAQVVALQAELTKVAD
                     EIFGNLTPWQKTLVARHPLRPYTLDFISYLFEEWTEIHGDRKFSDDPAIVAGFARFRG
                     APCAIIGHQKGRNTKEKIYRNFGQPRPEGFRKALRVMKLAEKFNLPIFTFIDTQGAYP
                     GLGAEERGQAEAIAVNLREMAMLSVPVIVIVIGEGGSGGALALGVGDRVFMLEHAVYS
                     VISPEGCAAILWKNPSAAAEAAAAMRITAIDLKRLNIIDDIVPEPNGGAHADPARAAE
                     LLAPYLEKALKELQKVKPAALPEERYKKFRKMGVFEKG"
     gene            6589..7137
                     /locus_tag="VNN25_09445"
     CDS             6589..7137
                     /locus_tag="VNN25_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91794.1"
                     /translation="MLATDAEAAAAGRSSLETWAKNEHMRHVPSAEWMSWKVDQDLRR
                     RTDILFSSFDGLPFDDPRRQPIENEFRALCRSIDRLADSARHTRNNHPPHDLADRIAW
                     GLNQAVSSLNSLDPHLMGRRYPFHTGERSKAEPIYGSLLAVIQHIDRVLTLIRTIDRN
                     VDEHLLDGLVQLETPLRSQPIA"
     gene            7134..7646
                     /locus_tag="VNN25_09450"
     CDS             7134..7646
                     /locus_tag="VNN25_09450"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH91795.1"
                     /translation="MSILDAVRPLFDHMEWADATVWRAVLATPSAADDAILHARLYHI
                     HATQHAFLQAWRGEEVIFKKADEFPGLDAVFQYARSFYAVAPAYVAALAPEAVDAELV
                     LPWSGYFAKRAGFAAAPSTLGETLLQLPSHSTYHRGQVNIRLRELGTTPPLVDFIAWV
                     WAGKPAPDWG"
     gene            7738..8187
                     /locus_tag="VNN25_09455"
     CDS             7738..8187
                     /locus_tag="VNN25_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXH91796.1"
                     /translation="MRFIAQTLIHTTPEQLFAFHERPDAFALLMPPWEKSRVIESAPN
                     LHIGSRAVVDAHIAPGISIRWESMHTAYDPPHSFEDQQVRGPFRSWRHRHIVEPHPDG
                     AMLIDDITYEPPLGILGRIFGKFIIERRLRRLFAYRHAVTREHCEAG"
     gene            complement(8196..>9169)
                     /locus_tag="VNN25_09460"
     CDS             complement(8196..>9169)
                     /locus_tag="VNN25_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91797.1"
                     /translation="VHVDSGFRAQLPKLDPASPHVISAQMRFSDLSLARQEVVIAGGA
                     VSDSISTELTPVVIAGELRGKNATLERCLSANGVPLKTRAVETPEAQVFLIREPNARE
                     FVAAIDPDTFTRRSWQATLRARKATPLDPGTSMRFTWPIVRRYQSPGHISSNIFDTSE
                     LFLAKEVGMTGVLTVSFDETAANFPLLPVERGSQRRFADAVAVAGLRAVTDGRRRAIV
                     LIVDCTFDLRKDRSLAQPEAVRHYLEEVGVPLFVWSVNGPRPDLEPAWGEIEDIGTPR
                     QFQMAVQRLRATLAAQHIAWVAADPLTALRAEADPRCGITTVAKFGR"
BASE COUNT         1797 a         2982 c         2706 g         1684 t
ORIGIN      
        1 tcccagaacg gaatgccctg gcggcggttg atgacgccgg gacggctgac cgggatgtac
       61 tgcttcgtgg caacgaaggc cccgacgccg gcgatgacgg cgagtgccag cacgacgtag
      121 cgtgcggtgc ggcggaagag cagcgcgatc aacgcgagga agaccaatac gatcgcggcg
      181 gcggcgatgg ggttcttgtc gccgaggagg aatggctctt tcgtctcgtc gaaggcggcg
      241 aagaccggca ggtacgtctt cggatcgcgc cgcagaaaat cgaaaatgcc ggtgcggccg
      301 ggatcgatgc cggtggagaa cgtcgaccac gacaccggcg tctgcgcggg aaccgtcgga
      361 cgcagcggac ggaacgttcc gagcgttcgc agccgcgcca gattcgggag cttcccttcg
      421 ttcatccact gctcggtgta gcgggcgtcg acgccatcaa aaccgaggac gatgaccttc
      481 tccttggcga aggccgcgga aggcaaaacg agcgacagaa gcaccgccag gagcgcgaga
      541 aatcgattga atcgcatcga acggcatcat acttgtaatc cgtcgagcgg aaaattgagg
      601 tgctgcatga gaacgcgtca actctttgct tcattggcag ttgtgctgct ggccgcctgc
      661 agtggcggtg agaaaaacgg atcggcgggc gaccagaaaa cgctcgtcat cgccctgaag
      721 tcgggaccga cgaatctgga cagccgcgtc ggcgccgaca acgcctccag ccggatgttc
      781 gacctggtct acagcggcct gatcaaggtc acgccggact tcaactacgc gcccgatctg
      841 gccacgaagt gggagacgcc cgacgacaag acgatcgtct ttcatctcaa tccgaacgcg
      901 aagttccaca acggccagcc ggtcaaagcg gccgacgtga agtggacgta cgagtcgctc
      961 atgaacccga cgttcgtgac gtcgaagcgt tccggctaca gcacggtcga tcacatcgag
     1021 acgcccgacg agcagacggt catcttcaag ctgaaggagg cgaacgccgg catcttcgac
     1081 aacctcaccg tcggcatcct ccctaccgga gcggacacga atgtctacaa gaccaggccg
     1141 atcggctgcg gaccgtacaa actggtcgag catcgtcccg acgaccggct ggagttcgag
     1201 gccttcgatc agtggcacgg cggcgcgccg aagatcaagc acatcactgt gcgcatcatc
     1261 cccgacgcca cgacttgcat cctggaaatg cgccgcggca cggtgaattt cgaggtcaac
     1321 attattccgt tcgagaacgt cgcagagttc gagaagaacc ccgacttcaa agtcgtgaag
     1381 agcaacggct cggtttacca atacctcgcc ttcaacatga acgacccggc gctggcgaaa
     1441 ccgctggtgc ggcaggccat cgcacacgcc atcgaccgcg accggatcat ccgcgacatc
     1501 cagcgcggct acgcgcaaaa gaccgagtcg atgctcgccg agggtcactg ggcgcgcgct
     1561 gccaaccttc cttcctatga ttacgacccg gtgaaggcga aacagctcct ggcccaggcc
     1621 ggctatccga acggtctcaa tctggtcttc aagacgtcga cggacgcgga ggcgaactca
     1681 cgggcgcagg tgatccagga catgatgaag aaggccggct tcaacgtctc gatccaatcg
     1741 aacgagatgt cgacgttctt tgccgacatc ggcaagggca atttccagat gtactcgctg
     1801 tcccgcaatg ggatcagcga tcccgatttc tattacgtca tcttctattc gaagaacacg
     1861 cctcccaatg gccagaatcg cgggtcatac gctaatccgc gtgtcgacca gttactggtc
     1921 gaaggtcact ccaccttcga ccgcgccaag cgaaagccgg tctacgacga gattcagaga
     1981 atcatcgcga cagacctccc ctacatttcg ctctacatgc agtccaacgt agcgatcatg
     2041 cgcaagaaca tcgacggcta tgtgcaatat ccggcagggt tctatacgtc gattccgcag
     2101 atgacgatga aatagccttg cgggtcaggt ctaaagtcta aagtgccgaa gacgatcgtc
     2161 gctaaggcga tcaggcctcc tcggcacttt agactttaga cctgacccag accttggtca
     2221 tgcagacgct gtacggatcc tcgaaatact gagcaaacgg cgggcaaggc tcgaagccga
     2281 agcggcggta aagctcgcgc gcgggagcaa atgcctccat cgtgccggtc tcgaggctca
     2341 cccgttcata gccgcgccgc ttcgcctcgt cgaggatctg ttccaggagt ctggaagcga
     2401 cgcccttccg ctgatgcgcc gccgcggtac gcatcgactt gatctcacca tgcgtcttgc
     2461 tcagttcctt gagagcgcaa cagcccagca gctcaccatc ctgacgggca ctccagaacg
     2521 taatagccgg atcgcgcagg ccgtcgagat cgagcgcatg aatactctcc ggcggcgagt
     2581 gcagcgccgc acttcgcaga tgctcgcgga ttaacgcaac gatgtccgct ctggagaggt
     2641 cgtcgatttg gatatccatg tctactttgc tgttcgcgca agtccatccg cacccgacgg
     2701 cccggcaacg agatacatca ccacgtgtcc cacaatcatg attacagcgt aggctacggc
     2761 gtctgcgacg atgggagcaa cgcctggcaa gaagaccatc cactccggaa acttcgcggc
     2821 gatgaagtag tctcgcggcg gtccgatgat ggtcacgatc gccgtcgaga tgacgagccc
     2881 tttcaacccg aagcggcgcg caatccgcca ggtcaccagg tagatcgggg agcacgaaat
     2941 tgcaacgccg atccccagca tcgtagcaag gaacggagtt gatgcgatcg gcatcagcca
     3001 ccaccccgcc cacacgcaga tagcgacgat gcccatcgtc acgaaggcga cggcaacccc
     3061 tcccaccact gcgccaagaa tgcggcgtga cgtcgcccgc gtgaggtagg cgacgatgac
     3121 cagctccacg aaatacgcgc aggtgatgag agtgagtggg tggtctgcca tgtcgtaatt
     3181 ccttgtttgc cgcgcatggg ggtcaggtct aaagtctaaa gtgccggatt agcttgatcg
     3241 cctaagggac gatcgtcttc ggcactttag actttagacc tgaccctgga agcaccgaaa
     3301 ccaacgagcc ttgcgcaatg aaacacgatg ccacggtttg aaggtgaccg gcgatttccg
     3361 gaccatacaa gacagcgaag tccgcgtcga tgtccgcgac gccgcctgaa accggccact
     3421 gcggatgggt tacctgatat tcgatcgtcg atccgtcgcg ctgtcgtgtg tagccccagt
     3481 agtgctccgt aatgaactct tcgagtgagc ccgctgccgg cagtgccgcg gggcgatcag
     3541 ccgcaaccgc aaagcgatgc caccggccgc cgagccgcca tgaataggag gccgtcaacg
     3601 caggctccaa cgagatcgtg tgccgcatcg gcagcgcgag atacggctcg ttgtacatcg
     3661 cacgcgctac gagcgcgatc gcgaaccgcg gaacaagctc gcggatgaac acgacgccgc
     3721 gacgcacctc ctccccgacc tcgcggcgga catagaagcg caggttgacc tcttcgaagt
     3781 tgcgatggaa cggaatcggg atgccgagga ctctcgtgtg cagaaactga aagccgacga
     3841 gactggcgaa gtaccgcccc tgccaggcgt cgagcgtcgt cccggccgga acgtacggct
     3901 gcagcacctt cgggtcaacc tcgtagttga tcatcgccag gttgtgccag gcggcggtga
     3961 ggaagggcgt gtgctccatt gttgccagcc tactattttc ttcgcggaag aacggtctcg
     4021 cgaagtcgcg aagcggttct aacttcgtgt cttcgcgtct tcgcgtgacc agcctttcag
     4081 gatgtgcgaa agtacccaac cttgcagaaa cccgcacagc agttcgatcg ctccctcgtc
     4141 gaaggtccca tcgttcccgc ggtctggcgg cttgcctggc cgaccatgct gcagaacatc
     4201 atcgccggcg tgcaggggat catcgaccac gtgatggtcg ggcactacgt cggctacacc
     4261 ggcaacgcgg ccatcggcgt cagttggcag gtcattctcg tcgtcatcgt cttcatcagc
     4321 tccctcttca ccggcatggc cgtgctggtg gcgcgattcg ccggcgcgaa cgagccggag
     4381 aaagtcaacc gcgtcgtcta tcaggcgttc ctcaccgccc tgctgatgtc ggccatcatg
     4441 ggagtgatcg gttacttcgc cgctcctgcc ctgctcgaca tcatcaaagc cgcgcccgag
     4501 gtgcagcatc aagcgctgcc gttcctgagg gcaatgttcg tcggcatctt cggcatgatg
     4561 ctgttcttca tgctcagcgg cgcctttcgc gctgccggcg atgccaaaac cccgctccgc
     4621 ctcggcgccg cgatcaccgt cctcacgatc gtcttcaacg tcatcctcat ccctcgcctg
     4681 ggcacgatcg gcgcggcgta cgggacgatc gccagcagca cactcgtgtc ggcctacggc
     4741 gtgtggcggt tgttcgctcc ccactccgtc attcgtttcc gcaagggcat ggacctctca
     4801 cccgacttca gcatcatccg ctcgctcttt cgattcggac tgcccaccgg agtgcaggga
     4861 atcgcgatga acatcggcgg cgtgctgatg ctgcgcttca tcggctcgct gcagcacagc
     4921 gccgcggcgc aggctgcgta cgcggtctgc tacaccgagt tgttctcgct gatcacgtgg
     4981 acgtccgtcg gcctcctcgg cgcctccgtc accatcgccg gccagaacct cggcgcaaac
     5041 agacccgacc gcacccgcct cggagtcgcc gtcgcctcgc gcatcggact cggcgtcgcc
     5101 gccgtgatag gcctgatgtt cttgctgatc ccgaacgtgc tgctcggcgt attcgggatg
     5161 aaggatccgc tcgttacctc gatcggcacg cagctcctcc gctacctcag cgtctccggc
     5221 ttcttcatca ccgtcgccct cagttacacg ggcggactgc aaggcaccgg cgacacgcgc
     5281 agcccgctgt acatctcgat cgtctcgcag atcatcatcc caatcggcat ctgcgcgatc
     5341 atccaaacga tgcgccccct ccaacctgcc gacatctggc gagccatcgt cctcggccac
     5401 ctaacccgcg ccatcctcag cgtgctccga ttccggcaag gaaagtggca gaacatcgaa
     5461 gtggatctgg agagggcgcc ggcgaactga caaattacgg cactttcaaa atggggtcag
     5521 gtccactttt gaaagtggcg acacaaccgc tcttcgaagg tgaaatgggt tcactttcaa
     5581 aagtagacct gaccccattt tgaaagtgcc gacttctacc ccttctcgaa aacccccatc
     5641 ttccggaact tcttgtacct ctcttccggg agtgcggcgg gtttgacttt ctggagctct
     5701 ttcagcgcct tttccagata gggcgccagg agctcagcgg cgcgcgcggg gtcggcgtgg
     5761 gcgccgccgt tgggctcggg gacgatgtcg tcgatgatgt tgaggcgttt gaggtcgatg
     5821 gcggtgatgc gcatcgcggc ggcggcttcg gcggcggcgg aggggttctt ccagaggatc
     5881 gcggcgcagc cttcggggga aatgaccgag tagacggcgt gctcgagcat gaagacgcgg
     5941 tcgccgactc ccagtgccag cgcgccgccg ctaccgccct ccccgatcac gatcacgatc
     6001 accggcacgc tcagcatcgc catctcgcgt agattgacgg cgatcgcctc ggcctggccg
     6061 cgctcttctg cgccgaggcc tgggtaggcg ccctgggtat cgatgaaggt gaagatcggg
     6121 agattgaact tctccgccag cttcatcacc cgtagcgcct tacgaaatcc ctcggggcgc
     6181 ggctggccga agttgcggta gatcttttcc ttcgtgttgc gccccttctg gtggccgatg
     6241 atcgcgcagg gggcgcctcg aaaacgtgca aatccggcaa cgatcgcggg atcgtcggag
     6301 aacttgcggt cgccgtggat ctccgtccat tcctcgaaaa ggtagctgat gaagtcgagc
     6361 gtgtacggcc gcagcgggtg ccgggccacg agcgtcttct gccacggcgt gagattgccg
     6421 aagatttcgt cggcgacttt ggtaagctcc gcctgaagcg cgacgacttg ggccggatct
     6481 ctctcagccg ccgttgcttc ctcgatctga cgcctgagct ggttgattgg ctcctcgaac
     6541 tgcgccggat ctagactcat aggtgcgtga ccctaacaga tgaacagcat gctcgcaact
     6601 gacgccgaag cggccgccgc agggcgctcc tcactcgaaa cctgggccaa aaacgagcac
     6661 atgcggcacg tgccctcagc ggaatggatg tcgtggaaag tcgatcagga tctccgccgc
     6721 cggaccgaca ttctcttcag ttccttcgat ggactcccct tcgatgatcc gcgccggcag
     6781 ccgatcgaga acgagtttcg ggcgctctgc cgctccatcg accggctcgc cgactccgcc
     6841 cgccacacgc gcaataacca cccgccgcac gacctggccg accgcatcgc ctggggcctc
     6901 aatcaggccg tatcgagcct caattcgctc gatccgcacc tcatggggcg ccgctatccg
     6961 ttccacacgg gcgaacggtc caaggctgaa ccgatctacg gctcgctgct cgccgtcatc
     7021 cagcacatcg accgcgtgct gacgctgatc cggacgatcg atcggaacgt cgacgagcat
     7081 ctcctcgatg ggctcgtgca actggagacg ccgctccgct cacagccgat cgcatgagca
     7141 tcctcgacgc cgtccgcccg ctcttcgacc acatggagtg ggctgatgcg accgtgtggc
     7201 gcgccgttct ggcgactcca tccgcggccg atgacgcgat cctgcacgcg cggctgtacc
     7261 acatccacgc gacgcagcat gcgttcctgc aggcgtggcg cggcgaagaa gtgatcttca
     7321 agaaagccga tgagttcccc ggtctcgatg ccgtcttcca atacgcgcgg tcgttctatg
     7381 cagtcgcgcc ggcatatgtc gcggcgctgg cacccgaggc tgtcgatgcg gagttggtgt
     7441 tgccgtggtc gggctacttc gcgaagcgcg ctggtttcgc tgcggcccca tcgaccctcg
     7501 gcgagacgct gctccaactc ccctcgcatt ccacgtatca ccgcggccag gtcaacatcc
     7561 ggcttcgcga gctcggtacg acaccgccgc tggtcgattt cattgcgtgg gtctgggcgg
     7621 ggaagcctgc gccggattgg ggttaaaaaa gactcaccac agagacacag aggacacaga
     7681 gaaacctctg tgctctctgt gtctctgtgg tgggcgcttc tgttttttcg acgatcgatg
     7741 agattcatag cccaaaccct catccacacc acgcccgagc agctcttcgc atttcacgag
     7801 cgtcccgacg ccttcgcgct gctcatgcct ccgtgggaga aatcgcgcgt aatcgagagc
     7861 gcaccgaacc tgcacatcgg cagccgcgct gtcgtcgatg cccacatcgc gcccggcatc
     7921 tccattcgat gggaatcgat gcacaccgcc tacgatccgc cccactcctt cgaagaccag
     7981 caagtgcgcg ggccattccg cagttggcgt catcgccaca tcgtcgagcc gcatcccgac
     8041 ggcgcgatgc tcatcgacga catcacctac gaacctcccc ttggcatcct cggccgcatc
     8101 ttcggcaagt tcatcatcga acgccgcctg cgccgcctct tcgcctaccg ccacgccgta
     8161 acgcgcgagc actgcgaagc gggttgataa acccctcagc gcccgaactt cgcaaccgtc
     8221 gtgatcccac accgcgggtc ggcctcggca cgcaatgccg taagcggatc ggcagcgacc
     8281 caggcgatat gttgcgcagc aagtgtcgcg cgcagccgct gcaccgccat ctggaactgt
     8341 ctcggcgttc cgatgtcttc gatttcgccc cacgccggct cgagatccgg acgcgggccg
     8401 ttgaccgacc atacgaagag cggcaccccg acctcctcca ggtagtgccg tactgcctcg
     8461 ggctgggcga ggctgcgatc cttgcggagg tcaaatgtgc agtcgacgat caggacgatc
     8521 gcgcggcggc gtccgtcggt gaccgcccgc aagccggcga ccgcgacggc atccgcaaaa
     8581 cggcgctgac tgccgcgctc caccggaaga agcggaaagt tcgcggcagt ctcgtcgaac
     8641 gacaccgtca gcacgcccgt cattcctacc tctttcgcca ggaacaattc cgaagtgtcg
     8701 aagatattcg aggagatatg ccccggtgac tggtaccggc gcacgatcgg ccaggtgaag
     8761 cgcatcgagg tgccgggatc gagaggcgtc gccttcctcg ccctgagtgt ggcctgccaa
     8821 ctgcggcgcg tgaaagtgtc cggatcgatg gcggcaacga attcgcgcgc attcggttcg
     8881 cggatgagga acacctgcgc ctcgggtgtt tcgacagcgc gcgtctttaa cggcacgcca
     8941 ttcgccgaga ggcatcgctc cagagtggcg ttctttccgc gcagctctcc ggcgatcacg
     9001 accggcgtca attccgtgct gatcgagtcg cttacggcac cgccggcgat gaccacctcc
     9061 tgacgcgcca gagagagatc gctgaatcgc atctgcgccg agatcacgtg cggactcgca
     9121 ggatcgagct tcggtaactg cgcacgaaat ccagagtcga catgaacgg
//
LOCUS       DATLVW010000306         6956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_464691, whole genome shotgun sequence.
ACCESSION   DATLVW010000306 DATLVW010000000
VERSION     DATLVW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464691"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VNN25_09465"
     CDS             complement(<1..634)
                     /locus_tag="VNN25_09465"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HXH91798.1"
                     /translation="MHILTSDQMKSIDRRATERFAVPSIVLMENAALAVVDAIGDHYP
                     DCDRAAIFCGLGANGGDGFAVGRHLEQRSVVPMVFIVGDREKIGGDAAANLAICERLG
                     LQIHEITDDDSLNEALVHASDADLVVDAIFGTGLNRAPEGLYAETIVSMMELGLPIVA
                     VDLPSGANASSAETFEPCVQAALTITFAAPKICHVFEPAAVSCGEVIVADI"
     gene            complement(719..1399)
                     /locus_tag="VNN25_09470"
     CDS             complement(719..1399)
                     /locus_tag="VNN25_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXH91799.1"
                     /translation="MFPTVPPPQQSLLVFARLPERGRVKTRLAQSLGEDRTLAIYEAM
                     LRDLLASIGESNAETEVEVLWAPSENANGEMLRRAFGDLPTAMQTGQSLGDRLSMSFS
                     ERFFFHATEKVIAIGVDDPRLPRDLIDHAFGLLDSCEWVIGPASDGGYYLIGCRASAF
                     DSDVFQGIEWGSSSVFASTLAKIRDWQNCVAVLPLRHDIDVAEDLDRYTAENVEGHLA
                     ALLARQSV"
     gene            1426..2229
                     /locus_tag="VNN25_09475"
     CDS             1426..2229
                     /locus_tag="VNN25_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXH91800.1"
                     /translation="MRTIALLTDFGTRDPYVAAMKGVIASRCEARIADLTHSIGPFDV
                     WEAAFFLRDVVAYWPEGTIFVCVVDPGVGSSRRILALESAGRFFLAPDNGLLHFVLED
                     VKRRSDSPVRLSEDAKGEPSDWRVRPTSAAFTVSVTNEALFLPRGSTTFHGRDRFAPV
                     AAAIANGIQLEELGPRISDPVMLDYENGAIIRIDRFGNCITDIVPPATPFAIVAGDWR
                     IDHIRTTYSGEGAFAIVGSTGCIEISVAGGSAATLLQLRRGDRITIVPA"
     gene            2226..3917
                     /locus_tag="VNN25_09480"
     CDS             2226..3917
                     /locus_tag="VNN25_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXH91801.1"
                     /translation="MTTDYRQSRELQPRRRGVRRFVLPLAITLAVLVALLFGVAWIPL
                     LQGRDAWLRGRNAEAIAIGEKWSRLDLWPSHYHQLLAAAYLSAGRDDAAAPHLGGIGA
                     VQFSVIPKDELARRLFARERYAAFLSYDAASRETSESADVPLYRAAALLATNRLADAQ
                     KAFASIDKAHVNAARYAAFQRTIVAHASGTVPYVFDRNGGTIAVIRAHAAASVDDDFA
                     PLVSREAGGLTIGAKLPQLGEDATIETTLDPFVQKAALKALGGFRGSLVAIDPRTNEI
                     LAIASSHGNGPLANLALEHQYEPGSVVKILTGLNAESGGVDLAALFPYTCKGFLEIDG
                     RHFGDWLPAGHGVLHSIDDALAVSCNVFFADVGIRLGSDSLKRFMTAAGFDTQANLGI
                     FQVPLGKTIGPVFNHFETAFFAIGLEHESMNALHVAMLASMMANRGVLTTPRLLVQRR
                     SILGDVVAAGPQQTSVRLAPQAAAEAIVHSMQAVVSDPKGTGRRALVPGISLAMKTGT
                     AGERKSGLEAVILAFAPAESPKIAFGVIAEDAGPAEFAGAKIAHDFLEAMQDRLK"
     gene            3943..4323
                     /locus_tag="VNN25_09485"
     CDS             3943..4323
                     /locus_tag="VNN25_09485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXH91802.1"
                     /translation="MPSGRDRRRSERFPTASIPVQVSDIDAELIDLSLSGAAVIHRSP
                     LPAGSPATLVFPSYGGIYIPCQILRSLVQVRRPESGPEYVFRTAISFAQMEPEQRVPL
                     HEFLEIQIARLKEKQDAAAKEEEV"
     gene            complement(4681..4962)
                     /locus_tag="VNN25_09490"
     CDS             complement(4681..4962)
                     /locus_tag="VNN25_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91803.1"
                     /translation="MKRATFAIAIVLIALACHRTNISRAQWQQMPQSEKTLYVRTLLG
                     HEKTREAKGGNALVFALPAEEYVKRIDEAYGRGDHRTVDAIFEAMGTRR"
     gene            complement(4959..6596)
                     /locus_tag="VNN25_09495"
     CDS             complement(4959..6596)
                     /locus_tag="VNN25_09495"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXH91804.1"
                     /translation="MKTYIAQLNPTVGALKANADLIRSAYGEGVAAGADVVLVPELAV
                     TGYPPRDLLDRETFVASALETRDALAAMTGDTTLVFGCITRNASWCGKPLHNSAIVAR
                     NGKIILEQHKSLLPTYDVFDEMRYFEPGRKVGVVGIAGVRAGIAICEDFWFDDEILGT
                     KIYCRNPIDEVARQGAEVILNISASPFNSGKRRSRYDLFSRIATRYDVPLVYVNQVGG
                     NDELLFDGSSIVIDAKGRTIFCAPAFEEHRSLVQLEGAPCDAVITMEQDAEIGHALIL
                     GLRDYIRKCGFKDVVVGLSGGIDSAVTAAIAVEALGAAHVTGIAMPSQFSSQGSVDDA
                     RALAESLGIAFHTVSIEPIYKPYDAAFQKLFGDDTFDITNENAQARIRGNLLMAWSNR
                     TGAMVLSTGNKSELAVGYCTLYGDMAGGLALLGDVYKTMIYRVARWLNRERALIPESS
                     ITKPPSAELRPNQTDQDSLPPYDVLDGILKLYIEKWQEVDAIVAAGFDRKTVERILKL
                     VDGNEFKRSQAAPTLRVSTKAFGSGRQMPIAQRWRRE"
     gene            6720..>6956
                     /locus_tag="VNN25_09500"
     CDS             6720..>6956
                     /locus_tag="VNN25_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91805.1"
                     /translation="MSDDFGTVNIKRGDRAREIEVLRQHYKVHRDTLTQMVPDAPTEH
                     LASEYKRLIADIDASVRKLDELDRVPSATAPGLVE"
BASE COUNT         1247 a         2084 c         2253 g         1372 t
ORIGIN      
        1 agatgtcggc gacgatcacc tcgccacagc tgacagctgc cggctcgaag acgtgacaga
       61 tcttcggtgc cgcgaaggtg atcgtgagcg ccgcctgaac gcacggctcg aacgtctccg
      121 cgctcgatgc gtttgcgccg ctgggaagat cgacggcgac gatcggcagt ccgagctcca
      181 tcatcgacac gatcgtctct gcgtacagcc cctcgggtgc ccgattgaga ccggtgccga
      241 agattgcgtc gacgacgaga tcggcgtcgg atgcgtgaac gagcgcttcg ttgagcgagt
      301 cgtcgtcggt gatctcgtgg atctgcaggc cgaggcgttc gcaaatggcg agattcgcag
      361 ccgcgtcgcc gccgattttc tctcgatcgc caacaatgaa caccatcggc acgacgctgc
      421 gctgttcgag atgccgcccg accgcgaagc cgtcgccgcc attcgcgccg aggccgcaga
      481 agatggcggc gcggtcgcag tcgggatagt gatcgccgat cgcgtcgacg acagccagtg
      541 ccgcgttttc catcagcacg atcgagggaa cggcgaagcg ctccgtggca cgccggtcga
      601 tcgatttcat ctgatccgag gtgaggatgt gcatggttat cgagtgaaag atacgctctt
      661 cgggggagtc gcgtttcggg ggtcgggggt cggagactct ccgctggcgc ttttggactt
      721 acacggactg cctggctaac aacgctgcga gatggccttc gacgttctcg gcagtgtatc
      781 ggtccagatc ttcggctaca tcgatgtcgt gccgtagcgg gaggacggcc acgcagttct
      841 gccagtcgcg gatcttggcg agcgtagacg cgaagacgga cgacgagccc cattcgatgc
      901 cctggaagac gtcgctgtcg aacgccgagg cgcggcaacc gatcaggtag tagccgccgt
      961 cggatgcggg gccgatcacc cattcgcagg agtcgaggag tccgaaggcg tgatcgatta
     1021 gatcgcgcgg caggcgtgga tcgtcgacgc caatggcgat gactttctcc gtagcgtgaa
     1081 agaaaaagcg ctcggagaac gacatggaga ggcggtcgcc gagggactga ccggtctgca
     1141 tggcggtggg gagatcgccg aaggcgcgcc taagcatttc gccgtttgcg ttctcgctcg
     1201 gagcccagag gacctcgacc tccgtttcgg cgtttgactc cccgatcgat gccagaagat
     1261 cgcgcagcat cgcctcgtag atcgccagcg tgcggtcttc gccgagcgac tgcgccaggc
     1321 gtgtttttac gcggccacgc tccggaaggc gcgcgaagac gaggaggctc tgctgcggtg
     1381 gtggaacggt cgggaacacc gcggaagtat agtttccgcg ctctgatgcg cacgatcgca
     1441 ctcctcaccg acttcggcac gcgcgatccg tacgtcgccg cgatgaaggg cgtgatcgcg
     1501 tcgcgctgcg aggcacggat cgccgatctg acgcattcga ttggtccgtt cgatgtttgg
     1561 gaggcggcgt tttttcttcg cgatgttgtt gcgtattggc cggaggggac gatcttcgtt
     1621 tgcgtcgtcg atcccggtgt cgggagctcg cgaaggattc tggcgttgga gtctgcgggg
     1681 cgtttttttc ttgcgccgga taatgggttg ctgcatttcg tcttggagga cgtgaaacgt
     1741 aggtcggact ctccagtccg actttctgag gatgcgaaag gagaaccgtc ggactggaga
     1801 gtccgaccta cgtccgctgc tttcacagtc tccgtaacga acgaagctct gttccttccg
     1861 cgcggctcga cgacgtttca tgggcgcgac cgctttgcgc cggttgcggc cgcgatcgca
     1921 aatgggattc agctcgagga gctgggtccg cgcatcagcg accccgtgat gctcgactac
     1981 gaaaacggcg cgatcattcg tatcgatcgc ttcggcaatt gcatcaccga catcgtcccg
     2041 ccggcgacgc catttgcaat cgtcgcaggc gactggcgca tcgaccacat tcgaacgact
     2101 tacagcggcg agggtgcctt tgcgatcgtg ggatcgaccg gttgcatcga gatctccgtt
     2161 gccggtggca gcgcggccac tcttttgcag ctccggcgcg gggatcgcat cacgatcgtt
     2221 ccggcatgac gacggattac agacaatcgc gagagttgca gccgcgacgc cgcggtgtgc
     2281 ggcgattcgt gttgccgttg gcgatcacgc tcgctgttct cgtcgcgctc ctattcggcg
     2341 tggcttggat tccgttgctg caaggccgcg acgcctggct gcgcggccgg aacgcggaag
     2401 cgattgcgat cggtgagaag tggtctcggc tcgatctctg gccgtcgcac tatcaccagc
     2461 tcctcgctgc agcctatctg tctgccggcc gcgacgacgc tgcggcgccg cacctcggcg
     2521 gcatcggcgc cgtgcaattc tccgtgatcc caaaggatga gttggcgcgg cggcttttcg
     2581 cacgcgaacg ctacgccgcg tttctttcct acgacgcagc ctcgcgcgag acaagcgagt
     2641 ccgccgatgt gccgctgtat cgcgcggccg cgctgctcgc gaccaaccgg ctcgcggacg
     2701 cgcagaaagc gttcgcttcg atcgacaaag cgcacgtgaa tgctgcccgt tacgcggcgt
     2761 tccaacggac gattgtcgcg catgcgagcg gcaccgtgcc gtacgtcttc gaccgcaatg
     2821 gcggcacgat cgcggtcata cgcgcgcatg ccgccgcatc cgtcgatgac gatttcgcgc
     2881 cgctcgtttc gcgcgaagcc ggcgggctga cgatcggcgc gaagctgccc caactcggcg
     2941 aagacgccac gatcgaaacg acgctcgatc cgttcgtgca gaaggctgcc ttgaaggcgc
     3001 tgggcggctt tcgcggctcg cttgtggcca tcgatccgcg tacgaacgag atcctggcca
     3061 tcgccagcag ccacggcaac ggtccgctgg cgaacctcgc gctcgaacat caatacgagc
     3121 cgggatccgt cgtcaaaata ctgactggac tgaacgcaga gagcggcggc gtcgatcttg
     3181 cggcgctctt tccctacacg tgcaaaggct ttctcgagat cgacggacgc catttcggcg
     3241 actggcttcc agccgggcac ggtgttctgc attcgatcga tgacgccctc gctgtttcct
     3301 gcaacgtctt cttcgccgac gtcggcattc gcctcggcag cgacagtctg aagcgcttca
     3361 tgaccgcagc cggcttcgac acgcaggcaa acctcggcat ctttcaggtt cctctcggca
     3421 agacgatcgg tccggtgttc aatcacttcg agaccgcgtt ctttgccatt ggtctggagc
     3481 acgagtcgat gaacgcgctg cacgtggcga tgctggcgtc gatgatggcg aaccgcggcg
     3541 tgctgacgac gccacgcctc ctcgtgcagc gccgctcgat cctcggcgac gtcgtcgcgg
     3601 ccggcccgca gcagacgagc gttcgccttg cgccgcaagc tgccgccgag gcgatcgttc
     3661 actcgatgca ggcggtcgtc tccgatccga aaggaacggg acggcgtgca ctggtgcccg
     3721 ggatttcgct ggcgatgaag accggcaccg cgggagagcg caagagcggg ttggaagcgg
     3781 tgatcctggc cttcgcgccg gccgaatcgc cgaagattgc ctttggcgtg atcgccgagg
     3841 atgccgggcc ggcagagttt gcgggggcga agatcgcgca tgattttctg gaagcgatgc
     3901 aggatcggct gaagtaacac cgcgtcgtgt cagaatacgg gaatgccctc cggccgtgac
     3961 cgccgacgat ccgagcgttt tccgacggca tcgatccccg tgcaagtctc cgacatcgac
     4021 gccgagttga tcgatctgag cctgagcggc gccgccgtca ttcatcgctc accccttccg
     4081 gctggctccc cggcgacgct cgtctttccg agctacggcg gtatctacat tccgtgtcag
     4141 atcctgcgct ccctcgtgca ggtgcggcgt cccgaatcgg gcccggagta cgttttccgc
     4201 acggcaattt ccttcgcgca gatggagccg gagcagcgag tgccgctgca tgagttcctc
     4261 gagattcaga tagcccgctt gaaggaaaag caggacgcgg cggcgaaaga agaagaggtt
     4321 tgaaattgcg gttgctgcat tgcctctaag ttctcgtgaa cagggtcgat taatatgtcc
     4381 atggatactt agagtactca atctcgtgac ctgaatatac ccatggccaa attaaatctg
     4441 cctcaggcta ggctcaaaat gtccatggcc ataattcgcc tggcaagaag gcaggcgtaa
     4501 tatgtccatg gatcgcttta tgagtcagtt cgactaccga atttgtccat agccaaattt
     4561 cgcgagtcaa aacgtctacc gaaatttgcc atggccaaat taaacgagtc aaagtggctc
     4621 ttttaatata tccatggata gattcgggtg tgccatgggc tcaaacggct ggccagtccc
     4681 ttatcgccgc gttcccatcg cctcaaaaat cgcatcgacc gtgcggtgat caccccgccc
     4741 gtacgcttca tcaatccgtt tcacgtactc ctcggcgggg agagcgaaaa caagggcatt
     4801 gccgcctttt gcttccctcg ttttctcgtg cccgaggagg gttcgaacgt agagggtctt
     4861 ctcgctttgc ggcatctgct gccactgcgc gcgagagatg tttgtgcgat ggcaggcgag
     4921 ggcgatgagc acgattgcga tcgcgaacgt cgcgcgcttc attcgcgccg ccaccgttgc
     4981 gcgatcggca tctgccgtcc gctgccgaag gctttggtcg atacgcgcag cgtcggggcg
     5041 gcctggctgc gcttgaactc atttccgtcg accagtttca ggatgcgctc gacggtcttg
     5101 cggtcgaagc cggcggcaac gatggcatcg acctcctgcc acttctcgat gtagagcttg
     5161 aggatgccgt cgagcacgtc gtagggtggc agcgaatcct ggtcggtttg attgggccgc
     5221 aattccgcgg aaggcggttt ggtgatcgat gattccggga tcagcgcgcg ctcgcggttc
     5281 agccaacggg ccacgcgata gatcatcgtc ttgtagacgt cgccgaggag tgcgaggccg
     5341 ccggccatgt cgccgtagag cgtgcagtag cccaccgcca gctccgattt gttaccggtg
     5401 gaaagcacca tcgcgccggt tcggttggac caggccatca gcagattgcc gcggatgcgg
     5461 gcctgcgcgt tctcgttcgt gatgtcgaag gtgtcgtcgc cgaagagctt ttggaacgcg
     5521 gcgtcgtacg gtttgtagat cggctcgatg gagaccgtgt ggaaggcgat accgaggctt
     5581 tccgcgagcg cgcgggcgtc atcgaccgag ccttgcgacg aaaactgcga cggcatggcg
     5641 atgccggtga cgtgtgcggc gccgagggct tcgacagcga ttgcagcggt gaccgcggag
     5701 tcgatgccgc cggaaaggcc gaccacgaca tctttgaaac cgcatttgcg gatgtagtcg
     5761 cgcaggccga ggatgagggc gtggccgatc tcggcgtcct gctccatcgt gatgaccgcg
     5821 tcgcacggcg caccttcgag ctgcaccagc gagcggtgct cctcgaacgc cggcgcgcag
     5881 aaaatcgtcc gccctttggc gtcgatgacg atcgaggagc cgtcgaagag gagctcatcg
     5941 ttgccgccga cctgattcac gtagacgagc ggcacgtcgt agcgcgtggc gatgcgcgaa
     6001 aagaggtcgt agcgcgaccg gcgtttgccg ctgttgaacg gcgacgccga gatgttgagg
     6061 atgacttccg ctccctgccg cgccacctcg tcgatcggat tacggcagta gatcttcgtg
     6121 ccgaggatct cgtcgtcgaa ccagaagtct tcgcagatgg cgatgccggc acggacgccg
     6181 gcgatcccga cgacgccgac tttgcggcca ggctcgaagt agcgcatctc gtcgaacacg
     6241 tcgtaggtcg gcagaagcga tttgtgctgc tccaggatga tctttccgtt gcgcgcgacg
     6301 atcgcggagt tgtgcagcgg cttaccacac cacgaggcgt tgcgcgtgat gcagccgaag
     6361 acgagcgtgg tgtcgccggt catggccgcg agcgcgtcgc gggtctcgag cgcggaagcg
     6421 acaaaggtct cgcgatcgag cagatcgcgc ggcggatagc cggtgacggc aagctcggga
     6481 acgagaacga cgtccgcgcc ggccgcgaca ccttcgccgt aggcacttcg gatcagatcg
     6541 gcgttcgctt tgagcgcgcc gacggtgggg ttgagctggg caatgtaggt tttcatgcgg
     6601 gcaatgcctg aaacacgcta gaccgtatcg cactttccgt gggcgcgaaa cgaacaaaca
     6661 acgcccaatt tgtcgcatcg cccgcttttt gcaaagatgc agagattcga ggtgaacgga
     6721 tgtctgacga tttcggaacg gtgaatatca agagaggcga tcgcgcccgt gagatcgagg
     6781 tgctgcggca gcattacaag gtccatcgcg acacgctgac gcagatggtt ccggacgctc
     6841 cgaccgagca tctggcgtcg gagtacaagc ggctcatcgc cgatatcgat gcctcggtcc
     6901 gcaagctcga cgagctcgac cgcgtgccgt cagcgactgc gcccggcctg gtggaa
//
LOCUS       DATLVW010000307         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_547648, whole genome shotgun sequence.
ACCESSION   DATLVW010000307 DATLVW010000000
VERSION     DATLVW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547648"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VNN25_09505"
     CDS             <1..937
                     /locus_tag="VNN25_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91806.1"
                     /translation="TYTTKLQAVTADDVGRVAKKYVQPNQVSVLVVGKEKDFDKPLST
                     LGAVTPIDITIPEPGAKPVAAGAAAGAAPAKPAVVGAGAAAAAPAKPTSSSPEGLALI
                     KKVQSFVGGKAKIDAVVATHSIGSMQAQGPQGPMDVEIESTVKYPDYSHRTVKTPMGE
                     IKMITTPDAAYMSGPMGAQDMPASQRATMRNESRADIISILKNIDNPKYIFTVAGTEK
                     VGTADAQALTVDADGASIKWLVDPTSGKILRRISQSPRGEAVTDYTEWKTFDGITLPI
                     SFITTTAGQPNGKGNLKSMEINPTIDPKLFEKPVN"
     gene            1140..1349
                     /locus_tag="VNN25_09510"
     CDS             1140..1349
                     /locus_tag="VNN25_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91807.1"
                     /translation="MAKNQATATAPGKSVIRDRSSVSGTFIERGKKKPLKARREILFP
                     LEPSTIGDDRITRAIDAVMSRKKQR"
     gene            1355..1969
                     /locus_tag="VNN25_09515"
     CDS             1355..1969
                     /locus_tag="VNN25_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91808.1"
                     /translation="MRPEEYDRNVFINCPFDDDYAPLFEAIVFAINDAGFRPRCARER
                     LDSSQVRLNKIVELISESRYSIHDLSRTALDTANALPRFNMPLELGIDLGCKAFHPEC
                     ANKSLLIFDSEQYRFQKFVSDISGQDIHQHSNSAKAAVTRVRDWLRTESGRTDLPGGA
                     AIYSRYENFRADQPAICAELKLELTQLTFRDFSFTIAHWLERHL"
BASE COUNT          456 a          694 c          550 g          327 t
ORIGIN      
        1 aacgtacacg acgaaacttc aggccgtgac tgcggatgat gtggggcgcg tggcgaaaaa
       61 gtacgtccag ccgaatcagg tctccgtcct cgtcgtcggc aaagagaagg atttcgacaa
      121 gcctctctcc accctcggcg ccgtgactcc aatcgacatc acgatccccg agcctggcgc
      181 gaagccggtg gcagcaggag ccgccgccgg cgcagcacca gccaagccgg cagttgttgg
      241 agccggtgcg gcagccgcgg ccccggcgaa gccaacgtcc agcagccccg aaggactggc
      301 gttgatcaag aaggtgcaga gcttcgtcgg cggcaaagcg aagatcgatg ccgtcgtggc
      361 aacgcactcc atcggctcca tgcaggcgca gggtcctcag ggtccgatgg acgtggagat
      421 cgaaagcacc gtgaagtatc ccgactacag ccaccgcacc gtcaagacgc cgatgggcga
      481 gatcaagatg attaccacgc ccgacgcggc atacatgtcc ggcccgatgg gcgcgcagga
      541 catgcccgcc tcgcagcgcg ccacgatgcg caacgagtcg cgcgccgaca tcatctccat
      601 cctcaagaac atcgataacc cgaagtacat cttcaccgtc gccggcacgg agaaggtggg
      661 aaccgccgac gcgcaagccc tcacggtcga cgccgacggc gccagcatca aatggctggt
      721 cgaccccacc tccggcaaga tcctccgccg catctcccaa tcccctcggg gcgaagccgt
      781 caccgactac accgagtgga aaacgttcga cggcatcacc ctccccatct ccttcatcac
      841 caccaccgcc ggccaaccca acggcaaagg caacctgaag tcaatggaga tcaaccccac
      901 catcgacccc aagctgttcg agaagccagt gaactaacct cgcgtaatca ctatgcaaag
      961 ggccgccgaa aggcggccct tttgtttgcg cccgcccgca tctatgtcgt agcgccgacg
     1021 gccccgtcgg ccgtaccgcc agcggcccgc tggcgactct ggcccgcgaa acgccgtccc
     1081 cgctccgagg tagaatgtct ttcggcgatt ggacgttttc gacttggaga ggaaaagcga
     1141 tggcgaagaa tcaagcaact gctaccgcac ctggcaagag tgtcattcgt gaccgctcca
     1201 gtgtgagtgg aacgttcatc gagcgcggaa agaagaaacc actcaaagcc cgccgcgaaa
     1261 tcctctttcc cctggaaccg agcacgatcg gtgacgacag gatcactcgc gccatcgacg
     1321 ccgtgatgtc gcgcaaaaag cagcggtagt cgcgatgcgt cccgaagaat acgatagaaa
     1381 cgtattcatc aactgccctt tcgacgacga ctatgcgccg ctgttcgagg cgatcgtctt
     1441 cgccattaac gacgcaggct tccgtccaag gtgtgctcgg gaacgtctcg actccagtca
     1501 ggtccgcctg aacaagatcg tcgagctgat ctccgagtcc cgctactcga ttcacgacct
     1561 ctcccgcacg gctctcgaca ccgcaaacgc ccttcctcga ttcaacatgc cgctcgagct
     1621 tggcattgac ctcggctgca aggcattcca cccggagtgc gcgaacaagt ctctcctcat
     1681 cttcgactcc gaacagtacc gattccagaa gttcgtttcc gacatcagcg gccaggacat
     1741 ccaccaacac agcaacagcg ccaaagcggc agtcacccgc gttcgcgact ggctacgcac
     1801 cgaatcaggc agaaccgacc tcccgggcgg agcggcgatc tacagccgtt acgaaaactt
     1861 ccgagccgat cagcccgcaa tctgcgccga actaaaactc gagctcacac agctcacctt
     1921 ccgggacttt tcgttcacaa tcgctcactg gctggagaga catttgtagc ccgtacgcct
     1981 tcgtccagta gacctcaggc gtagcctttg ccgggagcgg cgcggcg
//
LOCUS       DATLVW010000308         5885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_94698, whole genome shotgun sequence.
ACCESSION   DATLVW010000308 DATLVW010000000
VERSION     DATLVW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94698"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VNN25_09520"
     CDS             complement(<1..1022)
                     /locus_tag="VNN25_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91809.1"
                     /translation="MSLLRPLLILWIAVLTFPAGAASASDASASATATMRVLIASSKA
                     LHADVSAFRTVRKRFECVALIGSRAEVIAQAGGSATVLVDIDATGTLPVTHAACGFPS
                     HWLVRLREHSGRWQAERVWMPESDVAARIAAASDQSARLSILEEQPRYVTPELARSLA
                     ERGSVLLSQSKSPQEVADLAEAALTIAEETRSPNERARALVLLGRSFDRREQFTTAMA
                     LYQRARGIAEKTGDVALIASALSCVAAGRDRLGQPAVEEASEAYRLAKLVGDDRVAGA
                     SLYLLGVDDEYSGRYGDAIEKYTETARRAKRCGERLLEAVATAQTGVAYGYIGNRALA
                     YEFMDRG"
     gene            1215..1985
                     /locus_tag="VNN25_09525"
     CDS             1215..1985
                     /locus_tag="VNN25_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91810.1"
                     /translation="MLRLRRAEAPPIADFESFLAVVTGNAVNDYLRRRYPERTRLKNR
                     LRYRLTYSQDFALWTVAGIPICGLREWGTGDTVPSPLDATSGEFPDAAFHEPNGDVAL
                     AAVLRTIGRPVTLDDLVSLMENLWQKNRTPPAGVERAVDPAPSAAASLEQRDTLAMLW
                     REIQRLPQRQRAALLLNLRDEDGGNALALLVLAGVAPFDAVASAIGIAPDALASLWSR
                     MPLDDREIAAHLGLSRQQVINLRKAARRRLERFLRRQP"
     gene            1988..2995
                     /locus_tag="VNN25_09530"
     CDS             1988..2995
                     /locus_tag="VNN25_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91811.1"
                     /translation="MDEHLDADALQRFRSGAMSPAATAAAAHHLATCSECARRGTELV
                     SRAAAPGWLAHAVAGEAEHPDDETIVALAGDSLDGEQRRAVENHLRVCETCRREVAEL
                     RADAGAFHERSAAGTPVWRIAAIAAALVLAFVLFALLRKTYAPVDRTGQKVPPIDLRV
                     ERPAFLDDLRPRADVQRREGQHAGVETSMTPVGLVVESQTPQFAWTAEGRGYAVTVLE
                     GSHVVERSGVLTVPRWTPSRPLRRGQIYEWQVEIHGATTRIIPAPPAPPAFFRVADAD
                     SLQRIEDARRGHPGDHLLLGAAYARAGMQRQAEQELSLYLAQHPGDAAALRLLQSIRR
                     W"
     gene            3094..3651
                     /locus_tag="VNN25_09535"
     CDS             3094..3651
                     /locus_tag="VNN25_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91812.1"
                     /translation="MKYISLLVVMSLIFLPVNSYAGCPNSGISSQGPIPPAPNCADTA
                     YNYFDTSCAITSGNVTTGTMSCNNWSANQFTTGSGDADYQMTVPAGHGGSCKMVQVYI
                     DFNDPSAYASDALAGSVKVRHNGTVTYYSNFFTRYGNQSPLSCAIVSSGRFSAADGDT
                     IDVDFWSVNYTGATMKVTPAIIWDN"
     gene            complement(3915..4064)
                     /locus_tag="VNN25_09540"
     CDS             complement(3915..4064)
                     /locus_tag="VNN25_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91813.1"
                     /translation="MKRAVEQANHRLDAVFLRRGESVGDLLPQNVFWWRIKATAAAVC
                     AMVHQ"
     gene            4090..4212
                     /locus_tag="VNN25_09545"
     CDS             4090..4212
                     /locus_tag="VNN25_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91814.1"
                     /translation="MTKDFADFLESLNREGVACVVIGWIAALAHIPYRTTRDLG"
     gene            complement(4229..4304)
                     /locus_tag="VNN25_09550"
     tRNA            complement(4229..4304)
                     /locus_tag="VNN25_09550"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4268..4270),aa:Glu,seq:ttc)
     gene            complement(4323..4397)
                     /locus_tag="VNN25_09555"
     tRNA            complement(4323..4397)
                     /locus_tag="VNN25_09555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4363..4365),aa:Lys,seq:ttt)
     gene            complement(4494..4766)
                     /locus_tag="VNN25_09560"
     CDS             complement(4494..4766)
                     /locus_tag="VNN25_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HXH91815.1"
                     /translation="MWKAFSAIFVSVFLAEIGDKTQLATMLFASESENKKWIVFAASA
                     SALVLASAIGVLVGAQLERFVKPSTLKLVAGAGFIVIGIWTMMSRG"
     gene            complement(4857..5618)
                     /locus_tag="VNN25_09565"
     CDS             complement(4857..5618)
                     /locus_tag="VNN25_09565"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HXH91816.1"
                     /translation="MPALFISHGAPSIAIETDDFTRAVDAYGATLEGARAIAVISAHW
                     QERDVRVNAVAHPEMIYDFGGFPEALYKIRYEAPGDPTLAREIASLLGGAELEEQRGW
                     DHGLWVPLRIALPGANVPIVEIAQPFPTTPEQLMTIGRALAPLRDRGVVIIGSGGIVH
                     NLRRLHRPDKNAPVDDWAREFDTWVAKQLGTRDFAALMRYRELAPHADLAVPTPEHFE
                     PIFITAGATRDTDTLTTIYEGFHYGNLSMRAFAFG"
     gene            5649..>5885
                     /locus_tag="VNN25_09570"
     CDS             5649..>5885
                     /locus_tag="VNN25_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91817.1"
                     /translation="MRIRCLSLVVFVVTVSAALAETGQRLPTGTMLDPVAASHVVGNF
                     PLAMAVAPGGERVALLLCGWREQGVQIVDRKSGAV"
BASE COUNT         1034 a         1828 c         1964 g         1059 t
ORIGIN      
        1 ccgcggtcca tgaactcgta cgccagcgcg cgatttccga tgtagccgta ggccacgccg
       61 gtctgcgcgg tcgccaccgc ctccagcagc cgttcgccgc agcgtttcgc gcgccgggcg
      121 gtctcggtat atttctcgat cgcgtcgcca tacctgccgg agtattcgtc gtccacgccg
      181 aggaggtaga gcgaagcgcc ggcgacgcgg tcgtcgccta cgagcttcgc cagccgatag
      241 gcctcggagg cttcctcgac cgccggttgg cctaatcgat cgcgcccggc ggcgacgcag
      301 gaaagcgcgg aggcgatcag cgccacatca ccggttttct cggcgatgcc tcgcgccctt
      361 tgatagagag ccatcgccgt cgtgaactgt tcgcgcctgt cgaagctcct gccaagcaat
      421 accagcgcgc gcgctctctc gttcggactg cgcgtctctt cggcgatggt gagcgcggcc
      481 tcggcgagat cggcaacttc ctgcggcgat ttactttgtg acaagagaac gcttccacgt
      541 tcggcaagcg agcgcgccag ctcgggcgtg acgtaacgcg gttgttcctc gaggatcgac
      601 aagcgcgcag attgatcgct cgctgccgcg atccgggccg cgacgtccga ctcgggcatc
      661 cacacccgtt cggcctgcca acgtccggag tgctcgcgca gtctcaccag ccagtgggat
      721 gggaagccgc acgctgcatg cgtcaccggt agtgttccgg tggcgtcgat gtcgacgagt
      781 acggtcgcgg agccgccggc ttgcgcgatc acctcggcgc gagagccaat aagcgcgacg
      841 cattcgaaac gtttgcggac ggtccgaaac gcggagacgt cggcatgcag tgccttggag
      901 gaggcgatca ggacgcgcat cgtcgccgtc gcactggctg atgcatcgga agccgacgcg
      961 gcgcccgccg gaaatgtaag aacggcaatc cacaggatga ggaggggacg aaggagggac
     1021 acagggattc tcttaccggc catcataaaa cgattgctat acttcgccgc tcaacaaact
     1081 cggtgagtgc acctcttgaa gagactctcg aaagagcgca gcctgtcgtc gatcgcattt
     1141 tgttccgccg ttgcggcggt tggctgcgcc acgacgagat cgcggacatc cgttcctccg
     1201 ttctgctgcg cctgatgctg cggctgcgcc gtgccgaggc gcctccgatc gccgatttcg
     1261 aatcatttct cgccgtggtg accggcaacg ccgtcaacga ttacctgcgg cggcgctatc
     1321 cggaacgcac gcggctcaag aaccgcctgc gctatcgatt gacgtattcg caggatttcg
     1381 cattgtggac cgtggccggc atccccatct gcggactgcg cgagtggggg accggtgaca
     1441 ccgtgccgtc gccgctcgac gcaacgtcag gagaatttcc tgacgcggca ttccacgagc
     1501 ccaatggcga cgtggcgctg gcggccgtgt tgcgtaccat cggccggccg gtgacgctcg
     1561 atgatctggt gtcgctgatg gagaacctct ggcagaagaa ccggacaccg cctgccggag
     1621 tcgagcgcgc ggtcgatccg gcaccgagcg ccgccgcgag cctcgaacaa cgcgacacgc
     1681 tggcgatgct gtggcgggag atccagcgcc tgccgcaacg ccagcgcgcg gctctgcttc
     1741 tcaatctgcg cgatgaggac ggcggcaatg cgctggcgct tctggtcctt gccggtgtgg
     1801 ctccctttga cgcggtggca tcggcgatcg gcatcgcgcc cgacgcgttg gcgagtttgt
     1861 ggagcagaat gccgctggac gatcgggaga tcgcagcgca tttgggactc tcacggcaac
     1921 aggtgatcaa tctgcgcaag gcggcacggc gtcgtctgga aagatttctg aggcgccagc
     1981 cgtgaccatg gacgaacatc tcgacgccga tgcccttcag cgcttccgca gcggtgcgat
     2041 gtcgccggcc gccaccgcgg cagcagcgca tcatctggcg acgtgtagcg aatgcgcgcg
     2101 ccgcggtacg gagctcgtgt cgcgtgccgc tgctcccggc tggctcgccc acgccgtcgc
     2161 cggcgaagcg gagcatcccg acgacgagac gatcgtggcc cttgccggcg actcgctcga
     2221 tggcgaacag cgccgcgctg tcgagaatca cctgcgcgtc tgcgagacct gccgccgaga
     2281 agtggccgag ttgagggcgg acgccggcgc cttccatgag cgctcggccg ccggcacgcc
     2341 ggtctggcgc attgccgcca tcgcggcagc gctcgtactc gcctttgtcc tattcgctct
     2401 tctgcgcaag acgtacgcgc cggtcgatcg caccggtcaa aaggttccgc cgatcgacct
     2461 gcgcgtggag cggccggcat ttctcgacga cttgagaccg cgcgcggatg tgcagcgccg
     2521 ggaggggcaa cacgccggag tggagacctc gatgaccccg gtggggttgg tcgtcgaatc
     2581 gcagacgccg cagttcgcat ggacggccga gggacgcggg tacgcggtga ctgtcctgga
     2641 gggctcgcac gtagtcgagc gaagcggcgt gctgacggtg ccacgctgga cgccttcgcg
     2701 gccgctgcgt cgagggcaga tttacgagtg gcaggtggag atccacggcg ccacgacgcg
     2761 catcattccc gctcctcccg cgccgccggc gttcttccgc gtggcggacg ccgattccct
     2821 gcagcgcatc gaggacgcgc gccgcggcca tcccggcgat catttgctcc tcggcgcagc
     2881 gtacgcgcgc gccggcatgc agcgccaggc ggagcaggag ctttctctct atcttgcgca
     2941 gcaccccggc gacgcagcgg ccctgcggct ccttcaatcg attcgccgct ggtaagaaaa
     3001 ttcagcgccg gcggcaatat gaccctcccg tcggtcgtcc ctttcggaca gagccggtgt
     3061 gtgacccggc cgtatatctg aaaggagcag cacatgaaat acatcagcct gctggtcgtg
     3121 atgagtttga ttttcctgcc ggtgaactcg tatgcgggat gtcccaactc gggtataagt
     3181 tcccagggac cgataccgcc tgcacccaac tgcgccgata ccgcctacaa ctacttcgac
     3241 acatcctgcg ccatcacttc aggtaacgtc accaccggca cgatgtcgtg caacaactgg
     3301 tcagcgaacc agttcacgac cggcagcggc gatgccgatt atcagatgac ggtgccggcc
     3361 ggtcacggcg gctcgtgcaa gatggtccag gtgtacatcg atttcaacga tccgagcgca
     3421 tacgcgagtg acgcgctggc cggctcggtc aaggttcgcc acaacggcac ggtgacgtac
     3481 tacagcaatt tcttcactcg ctatgggaat caaagcccgc tctcctgcgc gatcgtgagc
     3541 agcggcaggt tcagcgccgc cgacggcgac accatcgatg tggatttctg gtcggtcaat
     3601 tacacgggcg ccacgatgaa ggtcacgccg gcgatcattt gggacaacta gcgcaaccgc
     3661 ccggcgcgcg cgggtcagcc gatccgcgcg cgtcaaaccg cgagagacgc gcaggatatg
     3721 cagtttaggt gtggcgtccc caacgggatt cgaacccgtg ttggcgccgt gaaagggcgc
     3781 tgtcctacgg gtggctcggg attgcgagaa cgccgggctc gccgagggcg attgatgaaa
     3841 ggctattctc agacgaagct gaagtagcat ctcttcatgg caagcgcgcg taaggcgacg
     3901 tcagagcgga aggatcactg atgaaccatc gcacaaacag ccgccgccgt cgccttgatg
     3961 cgccaccaga agacattctg cgggaggagg tcgccgacgc tctcgccgcg acgcaggaag
     4021 accgcatcga ggcgatggtt agcctgctcg acagcgcgtt tcaattgttg gtggtgagag
     4081 gattcagccg tgaccaagga ctttgccgat ttcctggaat cactcaacag agaaggcgtg
     4141 gcttgtgtcg tgatcggatg gattgcggcg ctggcgcaca tcccgtatcg aacgacgcgt
     4201 gacctggggt gaccgcgaaa acgccgagtg gcgtccccaa cgggattcga acccgtgttg
     4261 gcgccgtgaa agggcgctgt cctaggcctc tagacgatgg ggacgtatgc ggcgggcggc
     4321 cttggtgagc ccggtaggga tcgaacctac gaccaattgg ttaaaagcca actgctctac
     4381 cactgagcta cgggcccgta acctcaacga acaaaacctg cgagccgtgc tcgaagggtg
     4441 cggaagatac cacgccacgt gcggaacccg ctctccctgg atcttattgc gtttcagccg
     4501 cgggacatca tcgtccagat gccgatgacg atgaagcccg ctccggccac gagcttcagc
     4561 gtcgacggtt tgacgaagcg ctccaactgc gcgccgacca ggacgccgat cgcggatgcc
     4621 agcacgaggg cactggcgct ggcggcgaag acgatccact tcttgttctc cgattccgag
     4681 gcgaagagca tcgtggccag ttgagtcttg tcgccgatct cggcgaggaa gacggagacg
     4741 aagatggcgg agaaggcttt ccacatggga ttgagtgtat tggaaggacg gtccgcagat
     4801 gacgcagatg tggcagatgg ttcatctgtg tcgatctgcg tcatctgcgg attccctcac
     4861 ccgaacgcga acgctcgcat cgagaggttg ccgtagtgga agccttcgta gatggttgtg
     4921 agggtgtcag tgtcgcgcgt ggcgccggcg gtgatgaaga tgggctcgaa gtgttcgggt
     4981 gtggggacgg cgaggtcggc gtgcggtgca agctcgcgat agcgcatcag cgctgcaaag
     5041 tcgcgcgtcc cgagttgctt cgcgacccac gtgtcgaact cgcgggccca atcatcgacc
     5101 ggcgcgttct tgtccggcct gtggagtctg cggaggttgt ggacgatgcc gccgctgccg
     5161 atgatgacga cgccgcggtc gcggagtggg gccagcgcgc ggccgattgt catcaactgc
     5221 tcgggcgtcg tcgggaacgg ctgcgcgatc tcgacgatcg ggacgttcgc gccggggagg
     5281 gcgattcgga gcgggaccca gaggccgtga tcccatccgc gttgctcttc gagttctgcg
     5341 ccgccgagga gtgaggcgat ctcgcgggcg agagtgggat cgccgggagc ctcgtaacgg
     5401 atcttgtaaa gcgcctcggg gaagccgccg aagtcgtaga tcatctcggg atgagcgacc
     5461 gcgttcacgc gcacgtcgcg ctcctgccag tgcgcggaaa tgacggcgat ggcgcgtgcg
     5521 ccctcgagcg ttgcgccata ggcgtccacc gcgcgcgtga agtcgtccgt ctcgatggcg
     5581 atcgacggcg ccccgtgcga aatgaaaagt gcgggcatcg gagtgttcat accgatatca
     5641 tcgcctgcat gcgtattcgt tgcctatcgt tggttgtatt cgtcgtgacc gtcagtgctg
     5701 cccttgccga aacgggtcag aggctgccga cgggaacgat gctcgatcct gttgccgcgt
     5761 cgcacgtcgt cggcaatttt ccgctggcga tggcagtcgc gcccggtggc gagcgtgtgg
     5821 cgctgctact ctgcggatgg cgcgagcaag gcgtgcagat cgtcgatcgc aagtcggggg
     5881 cggtg
//
LOCUS       DATLVW010000309         4725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_139572, whole genome shotgun sequence.
ACCESSION   DATLVW010000309 DATLVW010000000
VERSION     DATLVW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139572"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VNN25_09575"
     CDS             <1..319
                     /locus_tag="VNN25_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91818.1"
                     /translation="VANVGTNTVTWNGSIAAGGTVTITIQAFVKQGTIGTTISNQATV
                     SYDSDANGTNDASRVSDDPSTAAPGDATSFQVVGSVPALSRMMLFLLAAALAVMSLIV
                     MRK"
     gene            330..797
                     /locus_tag="VNN25_09580"
     CDS             330..797
                     /locus_tag="VNN25_09580"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXH91819.1"
                     /translation="MPDARRKTRNEHSSGGAVIDMRDGKPFVAMIATRGRSRWGLPKG
                     AVSQGETSEQAALREVKEETGLDAEVIGLLDTIEYFFRAGDTLIKKSVDFYLMHHLGG
                     TLEPQLSEVDDVEWVPLSEGIERASFESERKLLSSALEQIELVVKRARASSSS"
     gene            complement(1000..1482)
                     /locus_tag="VNN25_09585"
                     /pseudo
     CDS             complement(1000..1482)
                     /locus_tag="VNN25_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
     gene            complement(1525..2385)
                     /locus_tag="VNN25_09590"
     CDS             complement(1525..2385)
                     /locus_tag="VNN25_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXH91820.1"
                     /translation="MALRPLRNATISFGLVSIPVRFYTATKSEDIHFNLLHESCGTRV
                     NRKWWCPHHEEIVSSDELIRAYAISKNKYVTFTDEEIETLETDDNRALEITEFVDLHE
                     IDPLMFEKAYFLGPSPGGGKTYRLLAQAMKKQDKVAVARWVAAGKEHLVVMRPYEKGL
                     VLHTMYYADEVRDFGAIDLEEGPVKEKEVALAEMLINELTEKKFDPLQFKDEYRQRLL
                     QRIKAKSQGKTIEPEEPEEEKGGEVIDLMEALRRSLSKGGAAGAAARRAPTKPASRSK
                     ARPAAKKRRA"
     gene            2523..>4725
                     /locus_tag="VNN25_09595"
     CDS             2523..>4725
                     /locus_tag="VNN25_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH91821.1"
                     /translation="MDSPIRSTLWSAYGVLVVLIVAGLALTVGILHLSNKQEYRIVEG
                     SGPLLDAVGAMNDDTLAIMSAARGFALTKRSQFQQQYDEAVRDFRKSSTNALRLATEP
                     RDAQRVAKMRKLFDEIKLYTDQEMAAALEGRDGNANEFMVAASQEHSSAPDYAGTMAD
                     EHARQEHADLQRITGMRSNMTMLMVIVSVVVIGIAAFLIWRIQQSLMASVERQVRRTE
                     TMIAGMSDGVMLVDAEGRTVFLNPAAQKLLNRSELGVPIFKHAETYGLVNDEGAPIDA
                     KELPAARALSTGRAVRDATMRIASGDRQIAISMSATPLHEDGHISGVIVTFRDITERL
                     RLEEELQLQAERAQILADAGAFFASNIDPVWVNQAIAERVAEVLGDWAAVILKSADTN
                     ELRVASIYHREMASLGLAWSYIYRQPLIVGEGIIGQVVSTGYPSLTQNLGTSSPVAPE
                     SELEGGYHAAPMKLASLLILPLRTRREMLGALVIAANDPERAMTDEKLPLAEILAERA
                     ALAIENAKLYTEQVDARRKVEDLSRLKDEFLSIASHELRTPVTSIKGYTQLAKALIRE
                     NDLVTSEEYLDVALDQIDRMSRLILELLDVSRIETGRLEIRREPIPWSTFVCDVVHRH
                     HTAVSDRRFQLNVPVSNKRVIGDRDRLEQVLGNLMENAVKYSPDSSEIMVSLEERDDQ
                     LVTSIADRGIGIPSDELGQVFERFHRGRQVSSTNYGGLGLGLYITKQIVERH"
BASE COUNT          869 a         1483 c         1431 g          942 t
ORIGIN      
        1 cgtggcgaac gtcgggacaa acacggtcac gtggaacgga tccatcgccg ccggcggcac
       61 ggtgacgatc acgattcaag ctttcgtcaa gcaaggaacg atcggaacga cgatctcgaa
      121 tcaggcgacc gtctcctacg actccgacgc caacggtacg aacgacgcct cgcgcgtatc
      181 cgacgatccg tcgacggccg cgcctggaga tgccacttct ttccaggtcg tcggcagcgt
      241 gcccgctctt tcgcgaatga tgctgttcct tctcgctgcc gcgctggcgg tcatgtcgct
      301 gatcgtgatg aggaagtaga atccatcgaa tgcccgacgc gcgacggaaa acccgtaacg
      361 agcactcctc ggggggcgcc gtgatcgata tgcgcgacgg aaagccgttc gtggccatga
      421 tcgccacgcg cggccgttcg cgatgggggc tgccgaaggg tgccgtctcc caaggcgaga
      481 cctcggagca ggcagctctg cgcgaggtga aagaggagac cggactcgac gccgaggtga
      541 tcgggctgct cgatacgatc gagtacttct ttcgggccgg cgacacgctc atcaagaaga
      601 gtgtcgattt ctacctgatg caccaccttg gcggcactct ggagccgcag ctcagcgagg
      661 tcgacgacgt ggagtgggtt ccgctttccg aggggattga gcgcgcgagt ttcgagtcgg
      721 agcggaagct gctttcttcc gcgctggagc agatcgagct ggtcgtgaag cgggcgcgcg
      781 cttcgtcgtc ttcgtagcgg tgcctgccgg tttctttttc gcgccgccct tctcttcctc
      841 gagtttgtcg gccagagtcg gcgctttgcc cgacgccata cggttgcttt gccacgtccg
      901 cttcgaagcg ccgatttcct cgacggtgag atcggaaact actgagcgcg gcgcgacttc
      961 cgtcacgtcg acatccgcat tcgcgaattc gtcgcggtgt ttcaggagca gccactcgtt
     1021 gcctttgccg tcgccgcgtc catgaatctt cacgagcgcc cactcgccga acatcttctt
     1081 gccgaacatg cgaaatgcaa gtttgccggt tttgagcgcc ttgaccggat cgtctccggg
     1141 ctcgatgaac tcccatgtgc cctcgtccca gatgatgacg ttgccgccgc cgtactgacc
     1201 ggctgggatc accccttcga acgacgcgta gtcgtacgga tggtcctccg tcatcatcgc
     1261 cagccgcttc gtggcgggat catacgaggg ccctttcggc accgcccacg atttgagcac
     1321 gccttccatc tccaggcgaa agtcatagtg cagccgcgat gcgtgatgct tctgaatgac
     1381 gaagatcggc agcttcgtcc ctttcggttt gccgcctgaa ggctcggacg tcacgccgaa
     1441 atcgcgcatg gcgcggtatt tcttgagaag gtggtcgggc atggttgcgg gctaccaggt
     1501 cgccaagtcg ccaggttgtt gtcttcacgc cctccgcttc ttcgcggccg gtcgcgcctt
     1561 gctcctggat gctggctttg tcggagcacg gcgcgcggca gcgccagcgg cgccgccttt
     1621 gctgagactt cggcgcagag cttccatgag gtcgatgacc tcgccgccct tctcctcttc
     1681 cggttcctcc ggctcgatcg tcttgccctg cgattttgct ttgatgcgct ggaggaggcg
     1741 ctgacggtac tcatccttga actggagcgg atcaaacttc ttctccgtca gttcgttgat
     1801 caacatctcg gcaagcgcaa cttccttttc ctttaccggc ccctcctcga ggtcgatggc
     1861 gccgaagtcg cgcacctcgt cggcgtaata catcgtgtgg agcacgagcc ctttctcgta
     1921 cggccgcatg acgaccagat gctctttgcc cgcggccacc cagcgtgcca cggccacctt
     1981 gtcctgcttt ttcatggcct gcgcgaggag ccggtacgtc ttcccaccgc ccggggaggg
     2041 cccaaggaag tacgctttct cgaacatgag cggatcgatc tcgtgcagat cgacgaactc
     2101 ggtgatctcg agtgcgcggt tgtcgtcggt ctcgagtgtc tcgatctctt cgtcagtgaa
     2161 ggtcacgtac ttatttttcg agatggcgta agcacggatc aattcgtcgg aggagacgat
     2221 ctcctcgtgg tgcgggcacc accacttgcg gttcacgcgc gtgccgcagc tttcgtgaag
     2281 cagattgaag tgaatgtctt ccgactttgt ggcggtgtaa aagcgaacgg gaatcgagac
     2341 caggccgaag gaaatcgtcg cgttgcgcaa cggtctgagt gccatgtgct cctccgtaaa
     2401 tcgatcttgc atccgccgtt ccagcgagtt gtccgtggaa gtcactgaaa cggcgtaact
     2461 tgaatgcctg gctggcccat ctgcaacggc gtcgatcttg caatagcggg ccgcggttag
     2521 agatggactc acccatccgc agcacgttat ggagcgccta tggggtgctc gtggtgctta
     2581 tcgtggccgg tctggcgctc acggtgggga tactccatct ttccaacaag caagagtacc
     2641 gcatcgtcga agggtcggga ccattgctcg acgccgtcgg cgcgatgaat gacgacacgc
     2701 tcgcgatcat gagcgcggcg cgcgggttcg ctctcaccaa gcggagccag ttccagcagc
     2761 aatacgacga ggcggttcgc gatttcagaa agtcgtccac gaacgcattg cgtctcgcca
     2821 ccgagccacg cgatgcccag cgcgtcgcca agatgcggaa gctcttcgac gagatcaagc
     2881 tatacaccga tcaggagatg gctgccgccc tcgaaggccg cgacggaaac gcgaatgaat
     2941 tcatggtggc ggcatcccag gagcacagtt cggcacccga ctacgccggc acgatggcgg
     3001 acgaacatgc ccgtcaggag cacgccgacc ttcagcgcat cacgggcatg cgctccaaca
     3061 tgaccatgct gatggtgatc gtcagcgtag tggtcatcgg cattgccgcg tttctgatct
     3121 ggcggatcca gcagtcgctc atggcatctg tcgaacgcca ggtacgccgt accgagacga
     3181 tgatcgccgg aatgtccgac ggcgtgatgc tcgtcgatgc cgaggggagg acggtgtttc
     3241 tcaatcccgc ggcgcaaaaa ctgttgaacc gctccgagct cggcgtgccg attttcaagc
     3301 acgccgaaac ctatggcctc gtgaacgacg aaggcgcgcc gatcgacgcg aaagaactgc
     3361 cggcggcgcg cgcactctcg acaggtcgtg cggtgcgcga cgcaacgatg cgcatcgcaa
     3421 gcggcgatcg ccagatcgcc atttcgatga gcgccacgcc attgcacgag gatggacaca
     3481 tctccggcgt catcgtcaca ttccgcgaca tcaccgaacg tctccgcctc gaggaagagc
     3541 tgcaacttca ggccgagcgt gcgcagatcc ttgccgacgc cggcgcgttc ttcgccagca
     3601 acatcgatcc tgtatgggtg aatcaagcca tcgccgagcg tgtggccgaa gtcctaggcg
     3661 attgggcggc ggtcatcctc aagtccgccg acaccaacga gctgcgcgtc gcttcgatct
     3721 accaccgcga gatggcctcg ctcggcctgg cctggtcgta catctatcgc cagccgctca
     3781 tcgtcggcga ggggatcatc ggtcaggtcg tgagcaccgg ctacccctcg ctcacccaaa
     3841 acctcggcac gtcgagcccg gtcgcgcccg agtcggagct cgaaggcggt tatcacgcgg
     3901 cgccgatgaa actggcgtcg ctgctgattc ttccgctgcg cacgcgccgc gaaatgctcg
     3961 gagcgctcgt catcgccgcg aacgatcccg agagggccat gacggatgag aagctgcctc
     4021 tggccgagat tctcgccgag cgtgcggcgc tggcgatcga aaacgccaag ctctacaccg
     4081 aacaggtcga cgcgcggcgc aaggtggaag acctctcgcg cctcaaggac gaattccttt
     4141 ccatcgcttc gcacgaactg cgcacgccgg tcacgtcgat caagggatac acgcagctcg
     4201 ccaaggcgct cattcgcgaa aacgatctcg tcacgtctga agagtatctg gacgttgcgc
     4261 tggaccagat cgatcgcatg tcgcgcctga ttctcgagct gctcgatgtc tcacgcattg
     4321 agaccggccg cctcgagatc cggcgcgagc cgattccttg gtcaaccttc gtgtgcgacg
     4381 tggttcaccg gcatcacacc gccgtcagcg accggcgttt ccaactcaac gttcccgtca
     4441 gcaacaaaag ggtcatcggc gatcgcgatc gtctcgagca ggtgctcgga aacctgatgg
     4501 aaaacgcggt caagtattcg cccgacagca gcgagatcat ggtgtcactc gaggagcgcg
     4561 acgatcagct agtgacgtcg atcgcggacc gcgggatcgg catcccgtcc gacgagctcg
     4621 ggcaggtctt cgagcgtttt catcgcggcc ggcaagtgtc gtcgacgaat tacggcggac
     4681 tgggccttgg cctctacatc accaagcaga tcgtcgaacg tcacg
//
LOCUS       DATLVW010000310         4004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_464763, whole genome shotgun sequence.
ACCESSION   DATLVW010000310 DATLVW010000000
VERSION     DATLVW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464763"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..878)
                     /locus_tag="VNN25_09600"
     CDS             complement(453..878)
                     /locus_tag="VNN25_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91822.1"
                     /translation="MDKVITALLPAFAAGVAIQQLVEILDPLFSKIGDSNRKKTLINV
                     ISAAVAVIFVWGGGLTVLAPLKPGINQWIDLAASALIVSSGTEGFNSIIKFMGYKKEE
                     QKAAAGDAKGSADGTIGLVNRQQPAPFDTAFSSGPVVPS"
     gene            complement(1161..2459)
                     /locus_tag="VNN25_09605"
     CDS             complement(1161..2459)
                     /locus_tag="VNN25_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXH91823.1"
                     /translation="MIEQAIGVYGGAELISALRGLCPARSFADGTERDRCRIVIIDTD
                     SYSLLPEPPRKSLVRILFDDDGASEARREGDIRVPRATFLAHPEDTLAVAAELAGTIV
                     HAAGLEAEVAYLTQIQELMTISDASAVSERITRTVLDMLGLTRGALFLHDPRLERYVV
                     SYTSDPKVRETGEFLPGIPPGLLQRALSSGTSFAADRDAGMIVMPMQAQHDLIGVIRV
                     SLDPDDAYDEDGAANVTRYLHSVGSVLSNIYQLTRSRDLAMRDDLTKAYNRRFFDSYL
                     DEEIERARRYAATCSIIFLDLDDLKTVNNAHGHLAGSRTLQEVAKRILAAVRGIDKVV
                     RFGGDEFCIILPQTDQEQAVAVAHRVRRAMMTAPFHLSDDIDIAISASFGIATFPTHG
                     LTKDDLIRQADAAMYRVKSTTKDAVGVALVGVESSREAIL"
     gene            complement(2569..3045)
                     /locus_tag="VNN25_09610"
     CDS             complement(2569..3045)
                     /locus_tag="VNN25_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HXH91824.1"
                     /translation="MSRDFKATAGVRLWAFRHVFLLYFTKPSVLEVNERRCEVRIPLN
                     WRTKNHLKSMYFGALCIGADVAGGLICFHLMSAMKVRLSFVFKAVRAEFLKRPEDDVH
                     FDCEDGEAIQALVRRAVSSGEREETTVHVTARVPKKLGDEPVAQFEMTLSVKAQNP"
     gene            complement(3042..3812)
                     /locus_tag="VNN25_09615"
     CDS             complement(3042..3812)
                     /locus_tag="VNN25_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH91825.1"
                     /translation="MRAIITGASSGIGAALAHELARRGRTLALLARREDLLDALVHEL
                     PTQSIALACDVTDAAAVHEAVKRGEEALGGPFDLAVANAGVGVPSHAAKFILADAELM
                     VRVNILGMFNLFDAVIPSMVERRSGRFAGIASIAGLRGLPATSVYSATKAAMQAFLEA
                     SRIDLLQYGVGVTTINPGFIETAMTEKNRFKMPFLMKVDDAARVIADGLERGKRVVEF
                     PRPMSLLMRFGRLIPDALWERATSPYARRKIEPEKVRR"
BASE COUNT          776 a         1190 c         1245 g          793 t
ORIGIN      
        1 cgcgaccacg ttcatagcta ctgccagagt gactacggcg gaaagtgaac agacgatgcg
       61 ttgagtcttc attagtcacc tcaatcacgt cgcggaaatg cgcgtagaac tattgacttg
      121 gacaccatcg cctaaggcgc cggtcttctg aaagatcgtt tgtgccgctc cgtcctgttg
      181 gagaaacacg gtcacccagt acacctgtcc cgccgccgca tttggcgatg ccatgcggac
      241 ctcccagctc aggatccttt gatcgaggcc agcggctggt ccgatgttgt actcctccga
      301 ctccggatcc tttgggacgg gttgagggtt ggacgcgtct gcgttccata ggaacatccg
      361 atgattcccg accagtgcac tgcccacgac gacaaccgcc gtgacggggc cgcctgcgcg
      421 cttaaccgtt acattgtcca ttggtctcct cctcaacttg gaacaactgg tccactagag
      481 aacgccgtat caaacggcgc aggttgctga cggttgacta agccaatcgt gccatcggcc
      541 gatcccttcg cgtccccggc cgccgccttc tgctcctctt tcttgtaacc cataaacttg
      601 ataatagaat tgaagccctc agtgccggaa cttacaataa gcgctgaggc ggcgaggtcg
      661 atccattgat tgatgcccgg tttgagtggc gcgagaacag ttaatccgcc cccccacacg
      721 aagatgactg cgacggcggc ggatatgaca ttaatcaatg tcttttttct atttgagtcg
      781 ccgattttgc tgaagagtgg atcaaggatc tctacgagct gctggattgc tacgccagcg
      841 gcgaatgcgg gaagcaatgc tgtgatcact ttgtccatgg atagtctcct gccagcctca
      901 gttgtcgttc ctattcgcga caatcttcaa ctccaaagga cttcgccccc tgagccgatt
      961 tctcgcgaag aagatgcgct ctgcttcgtc tataggaagg acagaagtcc gagattctcc
     1021 atgctggtgt aatttttctc actccgcatg accggcgcta aactaagaca gcggtcgccg
     1081 cagcgggcca ataactagcg ttcttgtctg cgtggcgcag gcgtggagtg ttgccgaggt
     1141 gctaaggaca ctgcggaagg ttagagaata gcctcgcggc tgctctccac gcccaccaac
     1201 gccacgccca ccgcatcctt cgtcgtcgac ttcacgcggt acatggcggc gtcggcctgg
     1261 cggatcaggt cgtccttggt gaggccgtgc gtcgggaagg tggcgatgcc gaagctggcg
     1321 ctgatggcga tgtcgatgtc gtcggagaga tggaacggcg cggtcatcat ggcccgccgc
     1381 acgcgatgcg cgacggcgac ggcctgctcc tgatcggtct gcgggaggat gatgcagaac
     1441 tcgtcgccgc cgaagcggac aactttgtcg atgccgcgca ccgcggccag gattcgcttg
     1501 gccacttcct gcagcgtgcg cgatccggcg agatggccat gcgcgttgtt gacggtcttg
     1561 agatcgtcga gatcgagaaa gatgatgctg cacgtggcgg cgtaacggcg cgcgcgctcg
     1621 atctcctcgt cgaggtagga atcgaaaaag cggcggttgt aagccttcgt cagatcatcg
     1681 cgcatggcca ggtcgcggct gcgcgtgagt tgatagatgt tgctgagaac ggaaccgacc
     1741 gaatggagat agcgggtcac gttcgcggcg ccgtcttcgt cgtaggcgtc gtccggatcg
     1801 agcgataccc gaatcacgcc gatgagatcg tgctgcgcct gcatcggcat gacgatcatg
     1861 ccggcgtcgc ggtctgcggc gaacgacgtt ccggaagaga gcgcgcgttg cagaagaccc
     1921 ggcggaatgc cgggcagaaa ctcgcccgtc tcgcgcacct ttggatcgct cgtgtagctg
     1981 acgacatagc gctcgagacg cgggtcgtgc aggaagagcg cgccgcgcgt cagtcccagc
     2041 atgtcgagca cggttcgtgt gatgcgctcg gagacggccg aggcgtcgga gatcgtcatc
     2101 agctcctgga tctgcgtcaa atacgcgacc tcggcctcca atcccgcggc atggacgatg
     2161 gtgcccgcga gctcggcagc gacggccagc gtgtcttccg gatgtgcgag gaacgttgcg
     2221 cgcggaacgc ggatgtcgcc ttcgcgccgc gcctcggacg cgccatcatc gtcgaacaga
     2281 atacggacga gactcttgcg cggcggctca ggaaggagcg agtacgaatc ggtatcgatg
     2341 atgacgatac ggcagcggtc gcgctccgtg ccgtcggcga atgagcgcgc cggacagagc
     2401 ccgcgaaggg cggagatgag ctccgccccg ccataaacgc cgatcgcttg ctcgatcatc
     2461 ggactgctag attagtcgat tccggggttg ggaggtttgt ttagcaccac agagacacag
     2521 aggacacaga ggtgaagagc ttcctctgtg tcctctgtgt ctctgtggtt atgggttttg
     2581 tgccttcacc gacaacgtca tttcgaactg tgccaccggc tcgtcgccga gttttttcgg
     2641 cacacgggcg gtgacgtgaa cggtcgtctc ttcgcgctct ccgctggaga ccgcgcggcg
     2701 gacgagcgcc tgaatggctt ccccatcttc gcagtcgaag tggacgtcat cctcggggcg
     2761 cttgagaaac tccgcgcgca ccgccttgaa gacgaaggag agccgcactt tcatcgccga
     2821 catcagatga aagcagatca agccgccggc gacgtcggcg ccgatgcaga gcgcgccgaa
     2881 gtacatcgat ttcagatgat tcttcgtccg ccagttcagc gggatgcgca cttcgcagcg
     2941 gcgctcgttg acttcgagca cggacggctt tgtgaagtag aggagaaaga cgtggcggaa
     3001 ggcccagagc cggacgccgg cagtcgcttt gaaatcgcgg ctcatcgtct gactttctcc
     3061 ggttcgatct tgcgccgcgc gtagggcgat gtcgcgcgtt cccacaacgc gtccgggatg
     3121 aggcgcccga accgcatcag aagcgacata ggccgcggga actcgaccac gcgcttgccg
     3181 cgctccaatc cgtccgcgat aacgcgcgcg gcatcgtcga ccttcatcag gaacggcatc
     3241 ttgaaccggt tcttctccgt cattgcggtc tcgatgaagc cgggattgat cgtcgtcacg
     3301 ccgacgccgt attgcagcag atcgatgcgg ctggcttcga ggaaggcctg cattgccgcc
     3361 ttggtcgccg aataaaccga cgtcgccggc agtccacgca gaccggcgat cgaggcgatg
     3421 ccggcgaaac gcccggaacg acgctcgacc atcgacggaa tcacggcatc gaagagattg
     3481 aacatgccga ggatgttgac acgcaccatg agctccgcgt cggcgaggat gaacttcgcg
     3541 gcatgcgagg gaacgccgac tccggcgttg gcgacggcga gatcgaaggg gccgccgagg
     3601 gcttcttcgc cgcgcttcac ggcctcgtgc accgcggcag catcggtcac gtcgcaggca
     3661 agcgcgatcg attgcgttgg caactcgtgg acgagtgcat cgagcaagtc ctcgcgccgg
     3721 gccagaagcg cgagcgtccg gccgcggcgc gccagttcgt gcgccaatgc agcgccgatt
     3781 ccgctggaag ctccggtgat gatggctctc acggtttgag tgtatcgcgg tagagactgc
     3841 tcaccacaga gacacaaagg acacagaggt tttctctgtg ctctctgtgt ctctgaggtt
     3901 caaacggaac gaacaaagtc aacgcgcctc atccgcgtcg cgccggaatc gaatgcgtag
     3961 tacttgtgat cgccgagttg ctcccggatg aactgttcca tctt
//
LOCUS       DATLVW010000311        10785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_301917, whole genome shotgun sequence.
ACCESSION   DATLVW010000311 DATLVW010000000
VERSION     DATLVW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301917"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VNN25_09620"
     CDS             <1..195
                     /locus_tag="VNN25_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91826.1"
                     /translation="GLEDEYLGLEDEYLDLEDEYLDLEDKYLALEDKYLDLEDKYLGL
                     EGKYLGLEGIYLALQSLVTY"
     gene            complement(421..3321)
                     /locus_tag="VNN25_09625"
     CDS             complement(421..3321)
                     /locus_tag="VNN25_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH91827.1"
                     /translation="MPRRILVAALLVTALLAPVARSQEMPEVARGLMEDADRARETNH
                     LDDAIAKYKRVIEVAPALASAYVNLGALYFQQGKIAEAYETFVRGVANAPSDRVLLTN
                     AAAAGQQLGKSEDALKYIDAAIEKNPRDAALHSLRSTILRSMNRNEDALAAIEKATQL
                     APDEAKYQFSLGNLLVPLGRKDDAIAAFRKAVDLDHNLLRAYYNLGAVLFEAGRYDEA
                     LSAYRMALAPIDQAFAKHQPVEAIHARAYANLGAIYLKQQKYPEAIDAYQKSASLEDS
                     TAARYNLGFLFFTTGKSDRAQDEYRKALSGDPTLPLAYLHLGTIAFRAAKYDEAIKSL
                     TDGMPHYDADSKHAALRLLGRAQLAHGDRAAARQSLEEALKVDANDSESLLLLGRMAR
                     HDKRLDDAKSLLDRAQAAAPTNAVVTLERALVARDSNDLVTERAALEALLARDPGRAP
                     LRAELNVVIARQNPSASTLRSLHVTSPEVSAVLDALDGKRDTAAATLAHSASPFARGD
                     AGILLWQLGRNSEAAPHLAAAHKALADWNEVALAAGEVALAEKRFDDAAELLSAIRCE
                     SALKPAENRGTTLDLVLGSAVDVCARARRSLTIALLSQAAGALDQAAKRSDEAGARRA
                     RQIAERVSSTDERERSLTLFLRGTADLVAGADAPGRDALTRAIAGDLPTAITAMAKKN
                     FEAAQAVEEAPQATEPSAPQQRNTVVVFLPDTPAENEKRLAEGATAIVGSLAAASSLP
                     LKPELFRRAEDARAFINANRESVGVVIANPEFLPGEFAPRYAFSHDGRTKYRRVVVVP
                     VRSQAKSLADLRGKMVSGVDALGDDGAGVTNRVADDLTALANALYGRTDAALVSESNP
                     LLAERAKDLRVIHTTAPQPMPVAAFGPMPAAERTALDEAFRSLSRATLAPLQMSSAVR
                     IGAEPRVAVKREIQTPQAAALGLKIEAPIELPLRVSVEFPRVEIPEDMFGPP"
     gene            complement(3321..4016)
                     /gene="purQ"
                     /locus_tag="VNN25_09630"
     CDS             complement(3321..4016)
                     /gene="purQ"
                     /locus_tag="VNN25_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXH91828.1"
                     /translation="MKFGVVLLPGSNCDHDAVHVTRDLLGADVEVLWHKETDLRGADC
                     VIVPGGFAYGDYLRAGALAKFAPVMEPIRSHAADGGLVFGICNGFQVLTEAGLLPGAL
                     MRNQHLRFLGRDVFVRVEETDTPFTGALERGAVLRMPIAHGEGNFFADDATLDSLERN
                     RQVIFRYCDAEGRLTSEANPNGSARNIAGICNSGRNVLGLMPHPERCAEALLGNSDGL
                     GVFQSIATSMAKV"
     gene            complement(4107..5288)
                     /locus_tag="VNN25_09635"
     CDS             complement(4107..5288)
                     /locus_tag="VNN25_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91829.1"
                     /translation="MKTSNVSSGRCASVSSPTPSSRTSRIRSTSSFALLLALAIPLTA
                     QPLTPVATHDQKIVKRWELPGDPHGIAIGPDGTVYAGLAQPQAVVAIDPKTGTVKKRL
                     VLDSAEIASTKELVTMRINRAGTRLYIANGSDESATILSLPDLAVRREITTEGEQIRD
                     ALPDPKGRYLYLLGRHVHVYDAEGEKELHTINLDDPMAIAASENGSLLAVVATEDFGN
                     TKATSVALYDTTSFAELAREPLQTDKTIEEALFAAGDQAFIALSRGSLFEKPISAGKP
                     VKQMTAGTNGQMRMAIDFGDLTNSDAICLPEGSGAQIATLASPDLLLFAERRCSASSA
                     FGGSSRHVTPASLYGIAAYAMAYDPKNNTVTVTDRAGFLTIYKAPKAAVDSRKTEPQR
                     H"
     gene            complement(5197..5445)
                     /gene="purS"
                     /locus_tag="VNN25_09640"
     CDS             complement(5197..5445)
                     /gene="purS"
                     /locus_tag="VNN25_09640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXH91830.1"
                     /translation="MKATVIVELKPSVLDPQGKAIQHSLSTLGFEGVEDVRAGKLFRV
                     TLADNGQPREDIERQLRQMCERLLANTVIENFSYSIDE"
     gene            5574..6080
                     /locus_tag="VNN25_09645"
     CDS             5574..6080
                     /locus_tag="VNN25_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91831.1"
                     /translation="MDNTVFLIPITISITFFGMVAFIVWTSSNAKSRRAALQADVQTR
                     LIDKFSNAPEFVDFLNSDTGKQFLTGVDKMPQLMARDRIVGGVSRGVIMTLLGAAFIA
                     IWLADSNIGFMYPGFILIGLGIGFFISTMISLKMSQKFGLIGNDMPTPREIVNHRLDR
                     RDSTETQA"
     gene            6166..6663
                     /locus_tag="VNN25_09650"
     CDS             6166..6663
                     /locus_tag="VNN25_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH91832.1"
                     /translation="MDEPAFEAFYRKTAGGVWSYIYRMTGDAAMADDLLQKTFFRFIR
                     SNPGITDPDHLRRYVYKTATNLVFDHFRETKRERTRAIEWTPAEVRPDRGDLRHDMAR
                     VFSDLKPQERALLWLAHVEGNSHEEIAEAIGVKSKSIKVMLFRARKRLGEMLTKKGLA
                     PEATR"
     gene            6660..7142
                     /locus_tag="VNN25_09655"
     CDS             6660..7142
                     /locus_tag="VNN25_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91833.1"
                     /translation="MTTECPFESNVIDAVTDGSWTESLRTHVASCADCAAAAEVAPWL
                     TRFAGMDERTHVLPDPAVLWLKAKVLQTSAAVERAALPITRLQIAAYLIIAAGWAAIL
                     TWKSAALQAWINHLSPGHMILGATGAETTSLSLSVLVAIIALASASVGVAMHTILAEE
                     "
     gene            complement(7128..7676)
                     /locus_tag="VNN25_09660"
     CDS             complement(7128..7676)
                     /locus_tag="VNN25_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91834.1"
                     /translation="MTNRRLLFVLFVLFLAATLWTGGLLQTHGKPLVRHPACHGIGTL
                     ELTAFPSAARQIVERWSGDRDKSGRSLNLLAIEDIHWDYGFIILYVAAFALAGLVGRF
                     LLDDWLASKGAALARAMILAGILDYLEDFGMTMELNTRYGIAPAVFTVSLVKWIIVFC
                     GIAYASAVILALPWRWRRLLLR"
     gene            complement(7673..9871)
                     /locus_tag="VNN25_09665"
     CDS             complement(7673..9871)
                     /locus_tag="VNN25_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH91835.1"
                     /translation="MGFMRWLQGVWHFVREPFLFLRPIRFVVLPLLVLLWALTASDEG
                     QDAIRAVIEFDPLCSQWIHVIWFVFWVFLLAVQAWYWSRQLLLIDFPRCVRRTADEDE
                     HQQLTKTTAALAEKFDSTEQWTPRIIGAFVFLMAIGALIKAAHSYVGNGNYTTHVATI
                     TCIAIGASLLLFLVIVVARRKILGPAQRVQSHHGLSLLTRGILVITILLSLFIVVLTA
                     VKPLAVGYLFPAPTLLMLSAAIWIGLGSWTIYWADMYHVPLIGILVALAFLFSPFNDN
                     HAVRKLTPAEGGADIRAFPRRTVADTFKDWYPQAARGDAKAPVFIVATEGGGIRAAYW
                     TAAVLAAIQDASPAFRDHLFAISGVSGGSVGATAFTAALADHRKAGGACPPATDQLPE
                     RYRFAVQQMLSFDALAPTLASLLHADLVQRFLPIPFIPDRARALETGWERGWENTMSD
                     RFFSGGFLKMYQGRGTASLPSLFLNGTVVETGRRTITSNCQITQCEIQDSDDILSKLE
                     SDVRLSTAAHNSARFTYVSPAGTIRNDACDASGHVVDGGYFENSGALTAADIVRAIRP
                     LGSFPLYVLVIRFQEKNGPPTHPEKFANEVLSPLRALLATRGARGELSVAQAKRMSDI
                     AECGEKDGTNAIRLRELVLIQDDAGGTKLPLGWLLAPRTRNAIDLQIGTTVPSGLSKV
                     IAEQVGKNVAIVEEIAKQLHTQLPLRPDLVQEAAQSSEHHVANPGQAVKP"
     gene            complement(9944..10687)
                     /locus_tag="VNN25_09670"
     CDS             complement(9944..10687)
                     /locus_tag="VNN25_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH91836.1"
                     /translation="MWDPTQYERFKDERAQPFHDLVALIEPRPHMRVVDLGCGTGELT
                     RALHEKLTAEETIGIDSSETMLLKADSFDSEVLHFERGEIEGFVADRPFDLVFSNAAL
                     HWIADHEHLFGGLVALLAPHGQLAVQMPANHDHPSHRIAAELAPSLGLESRTINVLPP
                     QRYASLLHHLGFARQHVRLQVYGHVLPSTRDVVEWTKGTTLTPYREALSPERYEEFLA
                     QYTERLIEQLGEERPYFYTFKRVLLWATF"
BASE COUNT         1994 a         3233 c         3515 g         2043 t
ORIGIN      
        1 ggcctcgaag atgaatacct cggcctcgaa gatgaatacc tcgacctcga agatgaatac
       61 ctcgacctcg aagataaata cctcgcgctc gaagataaat atctcgacct cgaagataaa
      121 tatctcggcc tcgaaggtaa atacctcggc ctcgaaggta tttatctcgc cctccaatct
      181 ctcgtaacct actgatcgga tgtcagttat cgacctaacg cgccggatcc aacgaccgag
      241 gcgccggaag gagtgcggtc gtctcgaccg cgggcactcg ggcgtcctcg cccgagtgca
      301 gtaggggcgg aggtcgaaac tcgtcaacgt tcaaggcttg ggtgaagtac ggcgtcgcag
      361 tttctgccgc gcaaaccatt ggaaaaccaa gggcatcccg tgctccgact tccacccact
      421 tcacggcggc ccgaacatgt cttccggaat ctcgactcgc ggaaactcca ctgacacccg
      481 aagcggtaac tcgatcggag cctcgatctt cagtccgagc gctgccgcct gcggcgtctg
      541 gatctcccgc ttcacggcga cacgcggctc ggcaccgatc cgaacagcgc tgctcatctg
      601 cagcggtgcg agcgtcgcgc gggagagcga gcggaaggct tcgtcgagcg cggtgcgctc
      661 cgccgcaggc atcggcccga acgcggccac cggcatcggc tgcggcgcgg tggtgtggat
      721 aacgcgcaag tccttcgccc gctcggcgag cagcgggttg gattcggata cgagagctgc
      781 gtcggtgcgg ccgtaaagcg cgttcgcgag tgcggtgaga tcgtcggcca cgcggttcgt
      841 cacgccggcg ccatcgtcgc cgagggcgtc gacgccgctg accatctttc cgcggagatc
      901 ggccagtgac ttcgcctggc tgcgcacggg cacgaccacg acgcgtcgat acttcgtccg
      961 gccatcatgg ctgaaggcgt agcgcggcgc gaattcgccc ggcagaaact cgggattggc
     1021 gatgacgact ccgacacttt cgcggttggc gttgatgaac gcgcgcgcat cctcggcacg
     1081 gcggaagagc tccggcttca acggcagcga cgatgcggcg gcaaggctgc cgacgatggc
     1141 cgtggcgcct tccgcgagtc tcttctcgtt ctccgccggc gtgtccggga gaaagacgac
     1201 gaccgtgttt cgttgctgcg gcgccgaggg ctcggttgct tgcggcgctt cttcgactgc
     1261 ctgcgccgct tcgaaattct tcttagccat cgctgtgatc gcagtgggca gatcgccggc
     1321 gatcgcgcgc gtgagcgcat cgcgcccggg cgcgtcggcg cccgcgacga gatcggctgt
     1381 gccgcggagg aaaagcgtga gtgatcgttc gcgctcgtca gtcgaggaga ctcgttcggc
     1441 gatctggcgc gcgcgccggg ctccggcttc gtccgaacgc ttcgccgcct gatcgagagc
     1501 gccggcggcc tgcgagagaa gcgcgatcgt cagcgacctg cgagcccgcg cgcaaacgtc
     1561 aacggcggat ccgaggacga gatcgagtgt cgtgccgcgg ttctccgccg gcttgagagc
     1621 agattcgcaa cggattgccg acaacaactc cgccgcatca tcgaatcgct tttccgcaag
     1681 cgccacttcg cctgctgcca gcgccacctc attccaatcg gccaatgctt tgtgagccgc
     1741 cgcgagatgc ggagccgcct cgctgtttcg cccgagctgc caaagcagga tgccggcgtc
     1801 gcctcgcgca aacggcgaag cgctgtgcgc aagcgttgct gcggccgtgt cgcgctttcc
     1861 gtcgagcgcg tcgagcacgg cgctgacttc gggactcgtg acgtggagag agcgaagagt
     1921 cgaggcgctc ggattctggc gagcaatgac gacgttcagt tcggcgcgta gtggcgcgcg
     1981 gccagggtcg cgcgccagca gcgcttccag cgcagcccgc tctgtgacga gatcattgga
     2041 atcgcgcgcc acgagcgcgc gctcgagtgt gacgaccgca ttcgtcggcg ccgcggcctg
     2101 agcacggtcg agaagcgatt tcgcgtcatc gagacgcttg tcgtgtcgag ccatccggcc
     2161 gaggagaagt agcgactcgg aatcgtttgc atcgaccttc aacgcttctt ccagcgactg
     2221 gcgtgcggcg gcgcgatccc catgcgcgag ttgggctcgc ccaagtaaac ggagcgcggc
     2281 gtgcttcgag tcggcgtcgt aatgcggcat tccgtcggtc agcgacttga tcgcctcgtc
     2341 gtacttcgct gcgcgaaacg caatcgtgcc gaggtgcagg taggccagcg gcaacgtcgg
     2401 atcgcccgac agcgcctttc gatactcgtc ctgcgcccgg tcgcttttgc cagtggtgaa
     2461 aaagaggaag ccgaggttgt aacgagcagc ggtgctgtct tcgagactcg cagatttctg
     2521 gtaggcgtcg atcgcctccg gatacttctg ctgcttgagg tagatcgcgc cgaggttggc
     2581 gtaggcgcgc gcgtgaatcg cttccaccgg ttgatgcttg gcgaaggcct gatcgatcgg
     2641 cgcgagcgcc atgcggtacg cgctgagcgc ctcgtcgtag cgccccgctt cgaagagaac
     2701 ggcgccgagg ttgtagtagg cgcgcagaag gttgtgatcg agatcgaccg cctttcgaaa
     2761 cgcggcgatc gcgtcgtcct ttcgcccgag cggaacgagc agattgccga ggctgaactg
     2821 atacttcgct tcgtccggcg cgagctgcgt ggccttctcg atggcagcga gcgcgtcttc
     2881 gttccggttc atcgaccgca gaatcgttga gcggagggag tgcaacgcgg cgtcgcgcgg
     2941 gttcttctcg atcgccgcgt cgatgtattt gagcgcgtcc tccgatttgc cgagttgctg
     3001 tcccgccgcc gccgcgttgg tcaagaggac ccgatcggaa ggcgcgttcg ctacgccacg
     3061 cacgaacgtc tcgtatgcct cggcgatctt tccctgctga aagtaaagcg cgccgagatt
     3121 gacgtacgcc gaggccagcg cgggagcgac ttcgatgacg cgtttgtact tggcgatcgc
     3181 gtcgtcgaga tgattcgttt cccgggcgcg atcggcgtct tccatcagtc cgcgcgcgac
     3241 ctcgggcatt tcctgagagc gcgcaacagg cgcgagcagc gcggtcacga ggagcgcggc
     3301 aacaaggatg cggcgcggca tcagaccttt gccatcgagg ttgcgatcga ctgaaagacg
     3361 cccagtccgt cgctgttgcc gaggagcgcc tcggcgcatc gttccggatg cggcatcaag
     3421 cccagcacgt tgcggccgct gttgcagatt ccggcgatgt tgcgcgcgga gccgttcggg
     3481 ttcgcctcgc tcgtgagccg tccctcggcg tcgcagtatc tgaagatgac ctggcggttg
     3541 cgctccaacg aatcgagcgt ggcgtcgtcg gcgaagaagt tgccctcacc atgcgcgatc
     3601 ggcatgcgca acaccgcgcc gcgctcgagc gcgccggtga acggcgtgtc ggtctcttca
     3661 acgcgcacaa aaacgtcgcg ccccagaaag cgcaggtgct gattgcgcat cagcgcccct
     3721 ggcagcaacc ccgcttccgt gagcacctga aatccgttgc agatgccgaa gacgagaccg
     3781 ccgtcagccg catgcgaacg gatcggctcc atgaccggtg cgaacttggc cagcgcgcct
     3841 gcccggaggt aatcgccgta cgcaaagccg cctggaacga tcacgcaatc ggcaccccgc
     3901 aaatccgtct ctttgtgcca gagcacctca acgtccgcgc cgagcagatc gcgcgtgaca
     3961 tgcaccgcat cgtgatcgca gttcgaaccg ggaagaagga cgacgccgaa tttcatgctg
     4021 gggggaagtt tatcgcgacg ctgcgcaaaa tcaggcagtg cgttaaccac agagacacag
     4081 aggtcacgaa ggttttctct gtgtcctcag tgtctctgtg gttcagtttt tcgactgtcc
     4141 accgccgcct ttggtgcttt gtagatcgtg aggaagccgg ccctatccgt gaccgttacc
     4201 gtgttgttct tcgggtcgta ggccatggcg taggcggcga tgccgtagag ggaggcgggg
     4261 gttacgtggc ggctcgatcc gccgaaggcg ctgctggcgc tgcagcggcg ctcggcgaaa
     4321 aggaggaggt cgggcgatgc cagcgtggcg atctgcgcgc cgctgccctc ggggaggcag
     4381 atcgcgtcgc tgttggttag atcgccgaag tcgatggcca tgcgcatctg gccgttcgtt
     4441 cctgccgtca tctgcttgac cggtttgccg gcagagatcg gcttttcgaa cagcgatccg
     4501 cggctgagcg cgatgaaagc ctgatcgccg gcagcgaaga gcgcttcttc gatcgtcttg
     4561 tcggtctgca gcggttcgcg ggccagctcg gcgaagctcg tcgtgtcgta aagcgcgacc
     4621 gacgtcgctt tcgtgttgcc gaaatcctcg gtggccacaa ccgcgagcag cgatccgttc
     4681 tcgctggcag cgattgccat cggatcgtcg agattgatcg tgtgcagctc tttctcgccc
     4741 tcggcgtcgt agacgtgaac gtgacgcccg aggaggtaga ggtagcgccc cttcggatcg
     4801 ggaagggcgt cgcggatctg ctctccctcg gtggtgatct cgcggcgcac ggccagatcg
     4861 gggagggaga ggatcgtcgc gctctcgtcg ctgccgttgg cgatgtagag gcgtgttccg
     4921 gcgcggttga tgcgcatcgt cacgagctct ttggtcgatg cgatctcggc ggagtcgagc
     4981 acgagtcgtt tcttgaccgt gcccgttttc ggatcgatgg cgacgaccgc ctgcggctgc
     5041 gcgaggccgg cgtagacggt gccgtcgggg ccgatggcga ttccgtgcgg atcgccggga
     5101 agctcccaac gcttgacgat cttctggtcg tgcgtggcga cgggagtgag cggctgcgcg
     5161 gtgagaggta ttgccagcgc cagaagcagc gcgaagctac tcgtcgatcg aatacgagaa
     5221 gttctcgatg acggtgttgg cgaggagacg ctcgcacatc tgccggagct gacgttcgat
     5281 gtcttcacga ggctgtccgt tgtccgcaag cgtgacccga aacagcttgc cggcgcgcac
     5341 atcttccacc ccttcgaagc ccagcgtgct cagcgaatgc tggatggcct ttccctgcgg
     5401 atcgagaacg gacggcttga gctcgacgat gaccgtggct ttcattgggc gaatgatact
     5461 tcctgccgtg gcgggtcacg gtttcacgcg gagacgcgga gggcgcggag aagacctgtt
     5521 aaccatccgc cagcctcacc gtattagctc ccgagttcca acctggaggt ttcatggaca
     5581 acacagtctt tcttatcccc atcaccattt cgatcacatt cttcgggatg gtggcattca
     5641 tcgtctggac aagctcgaac gcgaaaagcc ggcgcgcggc gcttcaggct gacgtgcaga
     5701 cgcggctcat cgacaagttc agcaacgcgc ccgagttcgt cgattttctc aactcggaca
     5761 ccggcaagca gtttctgacc ggcgtcgaca agatgccgca gctcatggcc cgcgaccgca
     5821 tcgtcggcgg tgtttcgcgc ggcgtgatca tgacgctcct cggcgcggcg ttcatcgcca
     5881 tctggctcgc tgacagcaac atcggcttca tgtacccggg cttcatcctc atcggcctcg
     5941 gcatcggctt ctttatctcg acgatgatct cactcaagat gtcgcagaag tttggcctca
     6001 tcggcaacga tatgccgacg cctcgtgaga tcgtgaacca caggctcgac cgccgtgact
     6061 cgacggaaac gcaggcgtga ggccaccgtg ttactggaaa atgctctgac ctcgccgttc
     6121 ggagcggccg tgcgaacggc ttctccgcaa cggcctgaaa gaccgatgga cgaaccagca
     6181 ttcgaggcct tctatcgcaa gacggccgga ggtgtgtggt cgtacatcta taggatgacc
     6241 ggtgatgccg cgatggccga cgatctgttg cagaagacgt tcttccgctt catccgcagc
     6301 aatcccggaa tcacggaccc cgatcatctt cgccgttacg tctacaagac cgcgacgaac
     6361 ctggtgttcg atcacttccg cgaaacgaag cgcgagcgga cgcgggccat cgagtggacg
     6421 cccgccgagg tgcggccgga ccgcggcgat ctgaggcacg acatggcgcg ggtcttctcc
     6481 gatctgaagc cgcaggagcg cgcgctgttg tggctggcgc atgtggaagg aaactcgcac
     6541 gaagagatcg ccgaagcgat cggcgtgaag tcgaagagca tcaaagtcat gctcttccgc
     6601 gcccgcaaac ggcttggtga aatgctgacg aagaaaggtc tggccccgga ggcgacgcga
     6661 tgacgacgga atgcccattc gaatcaaatg tcatcgatgc cgtcacggat ggttcgtgga
     6721 ccgagtcgct ccgtacgcac gttgcctcgt gcgccgactg cgccgcggcc gctgaggtgg
     6781 caccgtggct gacgcgcttc gcaggcatgg acgagcgcac gcatgtcctc cccgatccgg
     6841 cggtgttgtg gctgaaagcg aaagtgctgc agacgagcgc cgcagtcgaa cgcgccgcgc
     6901 tgccgatcac acgcctccag atcgccgctt acctgatcat cgccgccggc tgggccgcga
     6961 tcctgacgtg gaaatcagcc gctctccagg cctggatcaa tcacctctcc cccggccaca
     7021 tgatcctcgg cgcgaccggc gcagaaacga cctcactctc gctgagcgtc ctcgtcgcga
     7081 tcatcgctct ggcctcagcg agtgtcgggg tggcgatgca tacgatccta gcggaggagt
     7141 aggcgtcgcc accgccaagg caaagcgagg attacggcac tcgcgtaggc gataccgcag
     7201 aacacgatga tccacttcac tagcgagacg gtgaagactg ccggcgcgat cccatagcgc
     7261 gtatttagct ccatcgtcat gccgaagtct tcgaggtaat ccagaatgcc tgcgaggatc
     7321 attgccctcg ccagggcggc gccttttgag gccagccaat catcgagcaa aaaccttccg
     7381 acgagacccg ccagggcgaa tgcggcaacg tagagaatga tgaaaccgta gtcccaatgg
     7441 atatcctcga tggcaagtag attcagcgat cgaccggact tgtccctgtc accggaccag
     7501 cgttcaacga tctgacgggc cgctgatgga aacgcggtca gttccagagt cccgattccg
     7561 tggcaggcag gatgcctgac gagcggcttg ccatgggttt ggaggaggcc gcccgtccac
     7621 aaggtcgcgg caagaaaaag aacgaatagc acgaagagga gccggcgatt cgtcatggtt
     7681 tcaccgcctg accagggttg gcgacatggt gctcgctgct ctgggcggcc tcttgcacga
     7741 gatccggccg caatggcagt tgtgtgtgca gttgtttggc gatctcctct acgatcgcca
     7801 cgttctttcc gacctgctcc gcgattacct ttgacagccc agatggcaca gtcgtcccga
     7861 tctgcagatc gatcgcgttg cgggttcgcg gcgccagcag ccagccgaga ggcagcttcg
     7921 ttccgccggc atcatcctgg atgagcacga gctcgcgcag gcgaatggcg ttggtgccgt
     7981 ccttttctcc gcactcggcg atatcggaca ttcgcttcgc ttgggcaacc gagagttcgc
     8041 ccctcgctcc tcgagtagcc agcagtgcac gcagaggtga cagcacttcg ttggcaaact
     8101 tttcagggtg ggttggaggc ccgttcttct cttggaaacg gatgaccagt acgtacagcg
     8161 gaaaactacc gagtggccgg atcgcgcgaa cgatatcggc cgcagttagc gcgccggagt
     8221 tctcgaagta gccgccgtcg acgacgtggc cggaagcgtc gcaggcgtcg ttgcgaatcg
     8281 taccggcggg agagacgtag gtgaatcgtg cgctgttgtg cgcagcggtg ctcaagcgga
     8341 catcgctttc cagcttggag aggatgtcgt cgctatcctg gatctcgcat tgcgtaatct
     8401 ggcagttact cgtgatggtc cgacgacccg tctcgacgac agtgccgttc agaaagagcg
     8461 aaggcaatga tgcggtgcct cggccctgat acatcttcag gaagccgccg ctgaagaatc
     8521 gatctgacat cgtgttttcc catccgcgct cccacccggt ttccaacgct cgtgctcgat
     8581 caggaatgaa tgggatcggc agaaagcgct gcacgagatc agcatgaaga agcgatgcaa
     8641 gggtgggcgc cagtgcgtcg aacgacagca tctgttgcac agcaaaccga tagcgttccg
     8701 gcagttggtc agtcgcggga gggcaggctc cgccggcctt tcgatggtcg gcaagagctg
     8761 cggtgaacgc ggtcgcccct actgaaccgc ccgaaactcc actgatcgca aagaggtgat
     8821 cgcggaaggc aggtgaggcg tcctggatcg ccgccaggac cgccgctgtc cagtacgccg
     8881 cccgaatgcc accgccctcg gtggcgacga tgaacaccgg cgctttggca tctccgcgcg
     8941 ccgcttgcgg gtaccagtct ttgaacgtgt cggccaccgt gcggcgagga aaagcacgaa
     9001 tatctgcacc gccctcggcg ggtgtgagct ttctgactgc gtgattgtcg ttgaaagggc
     9061 tgaagaggaa tgccagggca acgaggatgc ctatcaacgg cacgtggtac atgtctgccc
     9121 agtaaatcgt ccagcttccc agtccgatcc agatggcggc ggagagcatg agaagcgttg
     9181 gtgcggggaa gagatatccg actgcaagag gcttcaccgc ggtaaggacg acgatgaaca
     9241 acgacagcag tatcgtgatc acgaggatgc ctcgagtgag aaggctgagc ccgtggtgtg
     9301 actggacgcg ttgcgctggt ccgaggattt tccttcgcgc aactacgatc acgagaaaga
     9361 gcagcaaact cgccccgata gcgatgcagg tgatcgtggc aacgtgggtg gtgtaattcc
     9421 cgtttcccac atatgagtgc gcggctttga tcagcgcgcc gatcgccatg agaaacacga
     9481 acgctccgat gattcgcggt gtccactgtt cggtgctgtc gaatttttct gctaacgccg
     9541 ccgtcgtttt cgtcaattgc tggtgttcgt cctcatcggc ggtccggcgt acgcaccgtg
     9601 ggaaatcgat gaggaggagc tgccgagacc agtaccaggc ttgcacagcc agcaggaaaa
     9661 cccagaacac gaaccagatc acgtggatcc attgggaaca aagtgggtcg aattcgatca
     9721 cggcccgaat tgcgtcttgt ccttcgtctg aggcggtcaa ggcccaaagc agaacaagga
     9781 gcgggagaac gacgaagcgg atcggtcgga gaaaaaggaa gggctcgcgg acgaagtgcc
     9841 agactccctg aagccagcgc ataaatccca tgacgactct cctgactaat tgacggacct
     9901 gttcaggaga tgcgcaggag gggtcgttcc ctacccacta tgtttagaac gtcgcccaga
     9961 gcagcacgcg cttgaaggtg tagaagtagg gccgttcctc gccgagctgc tcgatcagcc
    10021 gctccgtgta ttgcgcgagg aactcctcgt agcgctccgg tgaaagggct tcgcggtagg
    10081 gcgtcagggt cgtgcctttg gtccattcga cgacgtcgcg ggttgagggg aggacgtggc
    10141 cgtagacctg gaggcggacg tgttggcgcg cgaagccgag gtggtggagg agcgatgcgt
    10201 agcgctgggg agggagcaca ttgatcgtgc gcgattcgag gccgagcgag ggtgcgagct
    10261 cggctgcgat gcggtgagag gggtggtcgt gattggcggg catctgcacg gcgagctggc
    10321 cgtgtggggc gaggagggcg acgagcccgc cgaaaaggtg ctcgtgatcg gcgatccagt
    10381 ggagcgcggc gttggagaag acgagatcga atgggcggtc ggcgacgaag ccttcgatct
    10441 cgccgcgctc aaaatgcagg acttcggaat cgaacgagtc ggccttgaga agcatcgttt
    10501 ccgagctgtc gatcccaatc gtctcttcgg cggtgagctt ctcgtgcagc gcgcgcgtaa
    10561 gctcacctgt gccgcagccg aggtcgacga cgcgcatgtg cgggcgaggc tcgatcagcg
    10621 cgacgaggtc atggaacggc tgcgcgcgct cgtctttgaa tcgttcgtat tgggttggat
    10681 cccacatggg tgtcgggttt cgggagtcgg agacaagttg cggtagacgg ctcaggagtc
    10741 caggggttgg atgtttctcc gacccccgac tcccggctac cgacc
//
LOCUS       DATLVW010000312         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_220054, whole genome shotgun sequence.
ACCESSION   DATLVW010000312 DATLVW010000000
VERSION     DATLVW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220054"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VNN25_09675"
     CDS             <1..748
                     /locus_tag="VNN25_09675"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXH91837.1"
                     /translation="NIEDGRSCAMYEVPGQTQLAVEKSGSRTYMSGSEKQILEKFWEF
                     FDSDSRFITFNGRQFDGPFLMIRSAINGIVPKRDLVGYRYGYHPNCDLREVLNFFGTT
                     NARQFKFNLDLACKVFGVETSKGEGLDGRSVETWYRAGRHREIADYCLDDVRATAELY
                     EKVAPSLLIFNKEFRDAEERAARTRREIEAQPSLPMTFIESVTKTSIELSASLDGPEQ
                     VAVVSGSHQAIVLEKLIGTGEPEEEIPSGA"
     gene            769..2055
                     /gene="guaD"
                     /locus_tag="VNN25_09680"
     CDS             769..2055
                     /gene="guaD"
                     /locus_tag="VNN25_09680"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HXH91838.1"
                     /translation="MRVFRARVFTPIADPFATNPAKSYKYYDDGFLAIDGERIVGIGD
                     WRDVPRQSSSLTQLGRDIVIAPGFVDTHLHAPQLEMIGSYGGDLLEWLNRYTFPTEAK
                     FRDPVHAAMVARIFFEELLRNGTLCALIFSTIHEEATDIFFAEAERRGFRAIIGKTMM
                     DRNAPDSLLETARDSYEQSRALLQKWHKRGLLRYAITPRFAPTSTPELLEAAGQLKRE
                     FPDAYVHTHISENRNEVQWVHDLFPEAEYADVYDRYGLLDERTVLAHGVYLSEEELEL
                     LARRGSRIAHCPNSNLFLGSGLFRLHHTLEAGVAVGLGTDIGAGTTPSMFTTMADAYK
                     VQQVQGVSLSPIQLWYLATLGGARALSLGDECGSLEPEKSADFVMLDLKSTPLVELRS
                     ERASSVEDLLAGLIFTGDDRVVRECWIGGQMVASRS"
     gene            2513..2752
                     /locus_tag="VNN25_09685"
     CDS             2513..2752
                     /locus_tag="VNN25_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91839.1"
                     /translation="MPLFAPLGRHLSDLRPSAPVQRLHHSTLHLGKVGVNPTPAELPP
                     GARGFMAPRPAAAKTAEPESVADLFDKALDDVTKK"
BASE COUNT          608 a          973 c          911 g          643 t
ORIGIN      
        1 caacatcgaa gacggtcgct cctgcgcgat gtacgaagtc cccggacaaa cgcagctcgc
       61 agtcgagaaa agcggttcgc ggacgtacat gtccggcagt gagaagcaga tcctggagaa
      121 attctgggag ttcttcgaca gcgattcgcg cttcatcacc ttcaacggcc gacagttcga
      181 cgggccgttc ctgatgatcc gctcggcgat caacggcatc gtcccgaaac gcgatctcgt
      241 cggttaccgt tacggctacc atccgaactg cgaccttcgc gaagtactca acttcttcgg
      301 cacgacgaac gcgcgtcaat tcaaattcaa tctcgatctg gcctgcaaag tattcggggt
      361 cgagacgtcg aaaggtgaag gtctcgacgg acgttcggtc gagacgtggt atcgcgccgg
      421 gcggcatcgg gagatcgccg actactgtct cgatgacgtc cgcgcgacag ccgagttgta
      481 cgagaaagtg gcgccatcgc tgctcatctt caacaaagaa ttccgtgatg cggaagagcg
      541 tgcggccagg acgcggcgcg agatcgaggc gcaaccttca ctgccgatga ccttcatcga
      601 gtcggtgacg aagacatcga tcgagctctc cgcgtcgctt gatggacccg agcaggtagc
      661 tgtcgtcagc ggatcgcatc aggccatcgt gctcgagaaa ttgatcggga cgggcgagcc
      721 ggaagaagag attccgtccg gcgcctgatc gcgtgacaaa atgcacgcgt gagagtcttt
      781 cgggccaggg tcttcacgcc cattgctgat cccttcgcca cgaatccggc gaagtcctac
      841 aagtactacg acgatggttt tctggctatc gacggcgagc gcatcgtcgg aatcggcgac
      901 tggcgcgacg taccgcggca gagctccagc ctgacgcaac tgggacgtga catcgtcatt
      961 gcgccgggat tcgtcgatac ccatcttcac gcgccgcagc tcgagatgat cggatcgtac
     1021 ggcggcgatc tgctcgagtg gctgaaccgc tacacgtttc cgaccgaggc gaagttccgg
     1081 gatccggtcc acgcggcgat ggtggctcgc atcttcttcg aggagctgct tcgcaacgga
     1141 acgctttgcg cgctcatctt ctcgacgatt cacgaagaag ctaccgacat ctttttcgcg
     1201 gaagcggagc ggcgcgggtt ccgggcaatc atcggcaaga cgatgatgga ccgcaacgcg
     1261 ccggactcgc tgctggagac ggcgcgcgac tcctacgaac agagccgggc actcctgcag
     1321 aaatggcata agcgtggttt gctccgctac gccatcacgc cgcgcttcgc gccgacgtcg
     1381 acgccggaac tgctcgaggc cgccggccag ctcaagcgcg agttcccgga cgcctacgtt
     1441 cacacgcaca tttccgagaa ccgcaacgaa gtgcaatggg tccacgacct cttccctgag
     1501 gccgagtacg ccgatgtcta cgaccgctac ggcctgctcg acgagcggac ggtgctcgcg
     1561 cacggcgtct atctcagtga agaagagctg gagctgctgg cgcgccgcgg atcccgcatc
     1621 gctcactgcc cgaactccaa cctcttcctc ggcagcggtc ttttccgcct gcaccatacg
     1681 ctcgaagcag gtgtcgccgt cggtctcggc acggacatcg gagcgggaac gacgccgtcg
     1741 atgttcacga cgatggccga tgcgtacaag gtccagcagg ttcagggcgt ctcgctgagc
     1801 ccgatccagc tctggtacct ggccacgctc ggtggggcac gcgccctctc cctcggcgat
     1861 gagtgcggca gtctcgaacc agagaaatcc gccgacttcg tgatgctcga tctgaagtcg
     1921 acgccgctgg tggagctgcg tagcgagcga gcgtcatccg tcgaagacct ccttgccggc
     1981 ctgatcttca cgggcgacga ccgcgtcgtc cgcgaatgct ggatcggggg acagatggtg
     2041 gcatcgcgga gttaggttag gtcgcctcgg cagcgagaca ccggcgctcc ggagcccaag
     2101 taactggcta tcaacgagtt acaacttcca ggaaagcagc ggtctgaccc tctgccgccc
     2161 ggaacgaccg attcgagcat cttctctttg ctacgtagca tctttggatt gctacggaga
     2221 aacttttctt tcctacggag gatctttttt ttcctccgta gaaacttttt attgctccgt
     2281 aggatctttt ctttcctacg tagcatcttt ggattcctac gtagcatctt ttttccccta
     2341 cggagaagct ttctgatgct ccgtaggaac ttttcccccc tacggagaat ctttttgaag
     2401 ctccgcagca actttgcttt cctgcggaga atcttctctt tcctacgcag catcttttct
     2461 ttcctaagta gcaactttgg attgatccgt agcaactttg gattgctccg aaatgcccct
     2521 tttcgccccc ctcggaagac acctttcgga cctccgcccc tcagctccgg ttcagcgcct
     2581 tcatcacagc acgctccatc tcggcaaggt aggggtcaac ccgaccccgg ccgaactgcc
     2641 gcccggcgcg aggggtttca tggcgccccg tccggccgca gccaagaccg ccgagccgga
     2701 aagcgtcgcc gacctcttcg acaaagccct cgacgacgtg acgaagaagt agccaccgtt
     2761 atgtgccgcc cctcatttgg ccgggtgggg ggcggcgctt ttcggtcgga gtgtgcacgg
     2821 tgttcgaggt tgcgcgcatt gagcgagaga tcagagacta gagttcaacc cagagatcag
     2881 ggatcagaag agctccccag ccgctcctga tctctagtct ctaatccctg gtctctgctt
     2941 tcccgccgca agacgcgcga ggacaccgtt actggcagcg ctcttgcgcc ctctcagctt
     3001 ttggaggcac ccatgaaacg aataccagcg gtcttcatct tcgcggcgct cactactgtc
     3061 gccgcttttg gacaggatcg tactgtgtgg cggacgagtg cggacgtgca ggaaggcggg
     3121 cgcgggagca tcaca
//
LOCUS       DATLVW010000313         7611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_382857, whole genome shotgun sequence.
ACCESSION   DATLVW010000313 DATLVW010000000
VERSION     DATLVW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382857"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..833
                     /locus_tag="VNN25_09690"
     CDS             159..833
                     /locus_tag="VNN25_09690"
                     /inference="COORDINATES: protein motif:HMM:NF021567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HXH91840.1"
                     /translation="MVGEDIVVFQLVSRGDSKCDECGRELFKGNLLRKEGPRGLCIDC
                     ADLGHLAYVPAGDACITRRASNYSPLRAIVLRFSRSRKRYERQGILVSAEALARAEEE
                     CLDDAEVRERRREADAERRAERDAEYVGKFADEIRKRYPGAPAEAPEAIAVHACQVHS
                     KRIGRTASAKDFDPAAIDLAVQAYVRHRHTQYDALMAAGVDRPEARAQVREPIDVVLA
                     KWREKA"
     gene            complement(882..1694)
                     /locus_tag="VNN25_09695"
     CDS             complement(882..1694)
                     /locus_tag="VNN25_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH91841.1"
                     /translation="MLQLSNLTKTFFPATPNEVRALQGVSLDINEGSFVIVIGTNGSG
                     KSTLLNAAAGTFPLDHGTITLAGRDVTKWPEHRRAAMIGRVFQNPFSGTAPNMTVAEN
                     LALAARRGQPRGLGALLPAKLHAELRDRVATLNMQLETRMNNPIGTLSGGQRQALTLL
                     MATWRRPDLLLLDEHTAALDPKSADQIVRLTQRIVSEHHLTTLMVTHSMQQAATLGTR
                     IVMMNQGRVSADIGGADRRRIRAEDLVRRFEEIRRDEQLDVSAAEMLWEMYV"
     gene            complement(1718..2677)
                     /locus_tag="VNN25_09700"
     CDS             complement(1718..2677)
                     /locus_tag="VNN25_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91842.1"
                     /translation="MTLLVSAWTIGLILSLLALGVYISYRVFAFPDITADGSITFGAA
                     VAAAMLTRGMNPFAASGAAFLAGCLAGACTGVLHTRFRINQLLSGILVMTALYSINLH
                     VMGQSNLPLLSARTLTTYAERIGRRLSGGDTAMLLGWPVSARELGSLLLIAAVVLLVA
                     IAAYLFFRTNLGSAMRASGDNAQMIRALGVSVENMIILGLAISNGLIAFAGALLAQYQ
                     GFADVQMGIGMVVWGLASVIIGRALVGSDSLGSALTGTIMGSVLFRLIIAVALRWGLN
                     PNDLKLVTAIFVFGALVLPMALGVRTSRPRPSGRPRPDGLIPR"
     gene            complement(2674..3894)
                     /locus_tag="VNN25_09705"
     CDS             complement(2674..3894)
                     /locus_tag="VNN25_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXH91843.1"
                     /translation="MEIVKQRDGDFLHVRVSGRLDNHWSQPFDEAIAEMIREGAHHLR
                     VDLSAVNYVSSAGIGALMTAYRDTRELQGSFVITAASERVRMILQLVELESLLFGTAT
                     EPEAPTPAAEGPRAIDSEKALFECYPLDGARSTHRFIGDPTQIARGGYEETTPITASP
                     DLFALGVGALGNSYDDCRGLFGEFVAAAGSAAYMPTDGTSTPDYMTLSGGLVPEIQAL
                     YAIEFRGAPSHLLRFEASNPGGTVPLSEIVRVCASVAGTPDFGVVMAAEVSGLICTML
                     RRSPAQPDPARFDFPNVREWLSFMPEHEYARTSSIVVGFASSSPSEEARAFLRPVSDA
                     FRGHFHAAVTAFRSLARGKIEIAEVLAQAFQARSVFSVVHLLRDNRPIEGAGESEFQR
                     GACWIFPIANGDAS"
     gene            complement(3881..4918)
                     /locus_tag="VNN25_09710"
     CDS             complement(3881..4918)
                     /locus_tag="VNN25_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH91844.1"
                     /translation="MLIALRRVVLGLAVVIAASAILLLSDLQHRHPVARGANAASHRW
                     KLYFIQLNDVIDVEDAAAGVMEGLRESGLKEGRDFEVRTLNAHGDMATVSALIDAAVT
                     GGADMLITFSTPTLQAALRRAQNVPVVYNYVSSGLKAGVGKSNTDHAPNVTGVNLLPA
                     NDEAFAILKTYFPSVHRVGTLYCPAETNMVVSKEALDKTAKKLGYEVVYVAADTATDV
                     PNAAAALMGRDVDAVLQLPGNLTASAFGSISDAARQAHIPIVAFQKTQAIGGALIVVA
                     RDYRDSGRHAAHMAAQIMHGENPGRIPLEDFGKTRLIVNLDAARALNITLPPALVRSA
                     KEVIGAAVGNR"
     gene            5065..6360
                     /locus_tag="VNN25_09715"
     CDS             5065..6360
                     /locus_tag="VNN25_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH91845.1"
                     /translation="MRARVPSLIIILLIAITPLFAAAPAAPNYILLKPQRVFDAMSND
                     THEGWAVLVSGEKIVSVGPSLAIPPETVTIELPGMTLLPGLIDAHSHILLHPYNETLW
                     NDQVLHEPLAYRTIAATLHARDTLLAGFTTLRDLGTEGAGFADVSVKRAIDEGRIPGP
                     RLFVATRAIVATASYGPGPGGFAPEFEPLTKGGQEASGTAEILRAVREQIGHGADWVK
                     VYADYGRGSSPATPTFSLDELKTLVDEAHSANRPVSAHATTAEGMRRAILAGVDTIEH
                     GYNGTDEVFKLMAAHNVAYLPTLAAEEAYSEYFQSYKRGTKPYTKSMENVLRAFKLAM
                     ANNVTIGCGSDVGVFTHGDNYRELEWMVRAGMTPTQALLAATSVDAKILGQQDRFGRI
                     APGLLADLIAVAGDPTHDITTIEHVSFVMKGGQVVVGGK"
     gene            complement(6488..7288)
                     /locus_tag="VNN25_09720"
     CDS             complement(6488..7288)
                     /locus_tag="VNN25_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91846.1"
                     /translation="MRRGAVTFVIFFSITFIGTTASAQIVATRIAAAQFVIPAAGALD
                     GGNGTFFRSDITMVNYRSTVQNVRLQWLPQTVTGVGVPPTDITINASSGIASEDFVTN
                     ILHRSGLGAILVSGITPAGDFDPTAQLAATSRIWTFQPGSSGTTSQSLPVVSTADISS
                     TVQVVLGARRDSRFRMNVGIVNLSSSQQRFQVVSAGSNGTDVQVVDVEAMSMVLFRVS
                     GADSAIPLQIQVFNTSTTARSNSWIAYASSVDNITGDAWTTLGSNAPQ"
BASE COUNT         1379 a         2235 c         2632 g         1365 t
ORIGIN      
        1 cacacgcact ttctagtggt tccgaacccc gcaagcagct cagtaagcct gagaaccttc
       61 tcgtcggaga tgtactcggc ttcgatcgtt ctgctcatgg gaacagcgta aaacacttcg
      121 tgattgcctg atgcatttcg agcggcgaga atgatgtcgt ggtcggcgaa gacattgtgg
      181 ttttccagct tgtgagccgg ggcgactcaa agtgcgatga gtgtggccgt gagttgttca
      241 aaggcaacct gctcaggaag gaaggtccgc ggggactctg catcgactgc gcagacctcg
      301 ggcatctcgc gtacgttccc gccggagacg cctgcatcac gcgccgggca tcgaattact
      361 caccgttgcg cgccatcgtc ctgcggttca gccgctcgcg aaagcgatat gagcgccagg
      421 gcattctggt ctcggctgag gcgctcgccc gtgccgaaga ggagtgtctc gacgatgcag
      481 aagttcgcga gcggcggaga gaggctgatg cggaacgccg cgccgagcgc gacgccgagt
      541 acgtcgggaa gtttgcagac gagattcgca agcggtatcc gggcgccccc gcggaggcac
      601 cagaggcaat cgccgtgcac gcttgccagg tgcactcgaa acgaatcgga cgtactgcca
      661 gcgcaaagga cttcgaccca gccgcgatcg atctcgcggt gcaagcctac gtccgccatc
      721 gtcacactca atacgatgcg ctgatggctg ccggagtcga tcgccccgag gcgcgcgcgc
      781 aagttcgaga accaatcgat gtcgttctcg cgaagtggcg cgaaaaggcg tagttgcgac
      841 cgcagttcga tgacccggcc tacctgggtc gaacgtcgcc gctacacgta catctcccac
      901 aacatctccg cggccgaaac atcgagttgt tcatcgcggc ggatttcttc gaatctgcgg
      961 acgaggtcct cggcgcggat gcggcggcgg tcggcgccgc cgatgtcggc tgacacgcgg
     1021 ccctggttca tcatcacgat gcgggtgccg agggtggcgg cttgttgcat ggagtgggtg
     1081 accatcaggg tggtcaggtg gtgttccgag acgatacgtt gggtgaggcg gacgatctgg
     1141 tcggcgcttt tggggtcgag ggcggcggtg tgctcgtcca ggaggaggag gtcgggtctg
     1201 cgccaggtgg ccattaataa tgtgagggcc tggcgttggc cgccggagag ggtgccgatg
     1261 gggttgttca tgcgcgtttc gagttgcatg ttgagtgtgg cgacgcggtc gcgcagttcg
     1321 gcgtgaagct tcgccggcag cagggctccg agtcctcggg gctggccgcg gcgggcggca
     1381 agggcgaggt tttcggcgac ggtcatgttg ggtgcggtgc cgctgaacgg gttctggaag
     1441 acgcggccga tcatcgcggc gcggcggtgt tcgggccatt tcgtgacgtc gcgtccggcc
     1501 agcgtgatcg tgccgtgatc gagggggaag gtgccggcgg cggcgttgag tagtgtcgat
     1561 tttccggagc cgttggtgcc gatgacgatg acgaaggagc cttcgttgat gtcgaggctt
     1621 acgccctgaa gcgcacggac ttcgttgggg gtggcgggga agaaggtttt tgtcaggttg
     1681 ctaagttgga gcatggttgt gatccgattc tgttcagtca tcgcgggatc aaaccatccg
     1741 ggcggggacg cccggatggc cgcggacgag acgtccgcac tccgagggcc atcggcagga
     1801 ctaatgctcc gaagacgaag atggcggtga ctagtttcag gtcgttgggg ttgaggcccc
     1861 atcgcagggc gacggcgatg atgaggcgga agaggacgga gcccatgatc gtaccggtca
     1921 gggccgagcc gagactgtcg gagccgacga gggcgcggcc gatgatgacg ctggcgaggc
     1981 cccagaccac catgccgatt cccatctgga cgtcggcgaa gccttggtat tgcgcgagga
     2041 gggcgccggc gaatgcgatc aggccgttgg agatggccag gccgaggatg atcatgttct
     2101 cgacgctgac gccgagggcg cggatcatct gcgcgttgtc gccggaggcg cgcatggcgc
     2161 tgccgaggtt cgtgcggaag aagagatagg cggcgatggc gacgagcagg acgaccgcgg
     2221 caatcagcag caacgagccg agctcgcgcg cgctgacggg ccagccgagg agcatcgcgg
     2281 tgtcgccgcc gctgagccgt cggccgatgc gttcggcgta cgtcgtcagc gttctcgcgg
     2341 agagcagcgg caggttgctc tgccccatca catgaaggtt gatggagtag agcgcggtca
     2401 tgaccaggat tccggagagg agttgattga tgcggaatcg cgtgtgcagg acgccggtgc
     2461 atgcgcctgc gagacagccg gcaaggaacg ccgcgccgct ggcggcgaat ggattcatgc
     2521 cgcgcgtgag catcgccgcg gccacggcgg cgccgaacgt gatcgagccg tcggccgtga
     2581 tgtcgggaaa ggcgaagacg cggtagctga tgtagacgcc gagggcgagg agagagagga
     2641 tgagaccgat cgtccaggcg gagacgagaa gcgtcacgat gcgtctccat tcgcgatggg
     2701 gaagatccag catgcaccgc gctggaactc gctttcgcct gcgccctcga tgggacggtt
     2761 gtcgcgcagc aggtggacga cggagaagac gctccgtgcc tgaaacgcct gcgccaacac
     2821 ctcggcgatc tcgatcttgc cccgggcgag cgatcgaaat gcggttacgg ctgcgtggaa
     2881 gtgtccgcgg aaggcatcgc tgaccggccg gagaaacgcg cgcgcttctt ctgatggaga
     2941 cgatgatgcg aagccgacga cgatgctgct ggtgcgtgcg tactcatgct cgggcatgaa
     3001 cgacagccat tcgcgcacgt tgggaaaatc gaatcgcgcg ggatcgggtt gcgccggcga
     3061 gcggcgcaac atcgtgcaga tcagtcccga gacttcggcg gccatcacga cgccgaagtc
     3121 gggagtgccg gcgaccgagg cgcagacgcg gacgatctcg ctcagcggaa cggtgccgcc
     3181 tggattgctc gcttcgaagc gcaggagatg gctgggcgcg ccgcggaatt cgattgcgta
     3241 caacgcctgg atttccggaa cgagcccgcc ggagagggtc atgtagtcgg gcgtcgatgt
     3301 gccgtcggtc ggcatgtatg cggcgctgcc ggcagcggcg acgaactcgc cgaagaggcc
     3361 gcggcagtcg tcgtagctgt tgccgagggc gccgacgccg agggcgaaga gatcggggct
     3421 ggcggtgatc ggagtggtct cttcgtatcc gccgcgcgcg atctgggtgg gatcgccgat
     3481 gaagcggtgg gtcgaacgcg cgccgtcgag cggatagcat tcgaacaatg ccttctcgga
     3541 gtcgatggcg cgcgggcctt cggcggcagg cgtgggcgct tcgggctccg tcgccgttcc
     3601 gaacaacagc gactcgagct cgacgagttg cagaatcatc cgcacgcgct cggatgccgc
     3661 ggtgatcacg aacgagccct gcaactcgcg ggtgtcgcga tacgcggtca tcaacgcgcc
     3721 gatgccggcg gaactgacgt agttcaccgc ggagagatcg acgcgcaggt gatgcgcgcc
     3781 ttcgcgaatc atctcagcga tggcttcgtc gaacggctgc gaccagtggt tgtcgagcct
     3841 gcccgacacg cgcacgtgga ggaagtcgcc gtcgcgctgc ttaacgattt ccaacggctg
     3901 ctcctatgac ttcttttgcc gatcggacga gcgccggtgg aagcgtgatg ttcaacgcgc
     3961 gcgcagcgtc gagattgacg atcagacgcg tcttgccgaa atcctcgagc gggatgcgcc
     4021 ctggattctc gccgtgcatg atttgcgcgg ccatgtgcgc ggcgtgacgt ccgctgtcgc
     4081 gatagtcgcg ggctacgacg atcagcgcgc cgccgatggc ctgcgttttc tggaaggcca
     4141 caatcgggat gtgcgcctgc cttgcggcgt cactgatgct tccgaacgcg gacgccgtga
     4201 ggttgcccgg aagctgcagc acggcgtcga cgtcgcgtcc catcagcgcg gccgcggcat
     4261 tgggcacgtc ggtggcggtg tcggcggcga cgtagacgac ttcgtatccg agtttcttcg
     4321 cggtcttatc gagtgcttcc ttcgacacga ccatgttcgt ctcggccgga cagtacagcg
     4381 tgccgacgcg atgaaccgac ggaaagtacg tcttcagaat cgcgaacgcc tcatcgttgg
     4441 ccggcaggag gttgacgccg gtgacgttcg gcgcatgatc ggtgttgctc ttccccacac
     4501 ctgctttcaa tcccgacgag acgtagttgt agacgacggg aacgttctgc gcgcggcgca
     4561 gcgcggcctg gagggttggc gtcgagaagg tgatgagcat gtctgcgccg ccggtgacgg
     4621 cggcatcgat cagcgcggag accgtggcca tgtcgccatg cgcgttcagc gtccggactt
     4681 cgaagtcgcg accttccttc agccccgact cgcgcaaacc ttccatcacg ccggcggcgg
     4741 catcttcgac gtcgatgacg tcattgagtt gaatgaagta cagcttccat cgatggctgg
     4801 ccgcgttggc gccgcgcgcg acgggatggc ggtgctgcag atcggaaagc agcagaatcg
     4861 ccgacgcggc gatcacgacg gcgaggccga gcacgactct gcgtaaggcg atgagcacgg
     4921 aagggattcg tatcctcgct gtgcttcctg aaggtgtcaa gggtgtttca cgcgaagacg
     4981 cgaagacgcg aaggttcttt ctccgtgtcc tccgcgcctc cgcgtgaaac gcttttcgcg
     5041 acagaatacg tacggaggtt cttcatgcgt gctcgggttc cgtcgttgat catcattctt
     5101 ctcatcgcga ttacgccgct gttcgccgca gcgccagcgg caccgaacta catcctcctc
     5161 aaaccgcaac gtgtcttcga tgcgatgagc aacgacacac acgaagggtg ggccgtgctc
     5221 gtctccggcg agaagatcgt ctcggtcggg ccgagtctcg ccattccgcc ggaaacagtg
     5281 acgatcgagc tgccggggat gacgctgctc cccggactga tcgacgcgca ctcacacatc
     5341 cttcttcatc cgtacaacga gacgctctgg aacgatcagg tgctgcacga gccgcttgcc
     5401 tatcgaacga tcgccgctac gctgcacgcg cgcgacaccc tccttgccgg tttcaccacc
     5461 cttcgcgacc tcggcaccga aggagcgggt ttcgccgatg tctcggtcaa gcgcgccatc
     5521 gatgagggcc gcattcccgg tccgcggctc ttcgtcgcca cgcgcgcgat cgtggccacg
     5581 gccagctacg gtcccgggcc cggcggtttc gcgccggagt tcgagccgct gaccaaaggc
     5641 ggacaggaag cgagcggcac cgcggagatc ctgcgtgcgg ttcgcgagca gatcggacac
     5701 ggcgccgact gggtgaaggt ctacgctgac tacggccgcg gcagcagccc ggccacgccg
     5761 acgttctcgc tcgacgagtt gaagacgctc gtcgacgaag cgcacagcgc gaatcgcccc
     5821 gtgtccgcgc atgccactac agccgagggg atgcgccgcg cgatcctcgc cggcgtcgat
     5881 acgatcgagc acggctacaa cggcacggac gaggtcttca aactgatggc cgcgcacaac
     5941 gtcgcctatc tgccgaccct ggccgccgag gaggcgtatt ccgaatactt ccagtcgtac
     6001 aaacggggga cgaagcccta cacgaagagc atggagaacg tcctgcgcgc gttcaaactg
     6061 gcgatggcca acaatgtcac gatcggctgc ggcagtgatg tcggcgtctt cacgcacggc
     6121 gacaactatc gcgagctgga gtggatggtg cgcgccggca tgaccccaac gcaagccctc
     6181 ctcgctgcca catccgtcga cgccaagatc ctcggccagc aggaccgctt tggccgcatc
     6241 gcccccggcc tcctcgccga tctgatcgcc gtcgccggcg accccacgca cgacatcacg
     6301 acgatcgaac acgtgtcgtt cgtgatgaag ggcgggcagg tggtggtagg agggaagtga
     6361 ggacgagccg tttgtcggat cacgcgatcg gttgacacga cggggcgaca tccgaactgc
     6421 aaaagtgccg aaggtgatcg tcgctaaggc gatcagccca ctcggcactt tagacctgac
     6481 ccgcgcatca ctgcggcgcg ttcgagccca acgtcgtcca tgcgtcgccg gtgatgttat
     6541 cgacggacga tgcgtaggcg atccaggaat tcgatcgcgc ggtcgtgctg gtgttgaaga
     6601 cctgaatctg cagcgggatg gcgctgtcgg cgccgctgac tcggaacagc accatcgaca
     6661 tcgcctccac gtccaccact tgcacgtccg ttccattcga gccggcggag acgacctgga
     6721 agcgttgctg gctgctgctc aggttcacga ttccgacgtt catgcggaat cgcgagtcgc
     6781 ggcgcgcgcc aagaacgacc tggaccgtgc tgctgatgtc ggcggtggag acgacaggca
     6841 acgactgtga tgtcgttcca ctgctgccgg gctggaacgt ccagatgcga gacgtggctg
     6901 cgagctgcgc ggtcggatcg aagtcgccgg cgggggtgat acccgagacg aggatcgctc
     6961 ccaggcctga acgatggagg atgttggtca cgaagtcttc gctggcgatt ccggacgacg
     7021 cgttgatcgt gatgtcggtt ggcggcacac cgacgcctgt cacggtctgc ggcagccatt
     7081 gcaggcggac attttgaacg gtgcttcggt aattgaccat cgtgatatcg ctgcggaaga
     7141 acgttccgtt gccgccatcc agtgcgccgg cggctgggat gacaaactgg gccgctgcaa
     7201 tgcgggtggc gacgatctgt gccgaggcgg ttgtcccgat gaaagttatg ctgaaaaaga
     7261 tgacgaaagt gacagctcct ctacgcatga gatcctcccg cgccgtgcag tgcgaagatc
     7321 gagccggtcg ctttgccgag ctcggcaggg agactgatcc gcagatggcg cagatgagca
     7381 cagattaaag atctgcgatc catctgcgac atctgcggat agagctaaac ttccgtcatg
     7441 aaaaacatga aagtcggtat tctcgggtcg ggagacgtcg gcaagtcgtt cgcgcacgcg
     7501 tttggcgcgc tcgggcatga agtgaagatc ggatcgcgct cgccggagaa gctgcgcgag
     7561 tttgtcgctg gtgagggcga gcgcgtttcc agcggcacgt tcgaagaggc c
//
LOCUS       DATLVW010000314         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_302167, whole genome shotgun sequence.
ACCESSION   DATLVW010000314 DATLVW010000000
VERSION     DATLVW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302167"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..595)
                     /gene="hisIE"
                     /locus_tag="VNN25_09725"
     CDS             complement(11..595)
                     /gene="hisIE"
                     /locus_tag="VNN25_09725"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HXH91847.1"
                     /translation="MNDLKFDANGLIPAVIQDARTGEVLTLAYMNQESLDRTRESGET
                     WLWSRSRNELWHKGATSGNTQRVVAITEDCDADALVVSVIPNGPACHTGARSCFAGTP
                     PRTLDRLMNVLRDRRENGPEGSYTAKLFSGGRARIVKKIGEEATEVVIAALSETRDRM
                     ISEIADLVFHVTVLMSDEGIDWSEVEGELAKREK"
     gene            complement(692..1486)
                     /gene="hisF"
                     /locus_tag="VNN25_09730"
     CDS             complement(692..1486)
                     /gene="hisF"
                     /locus_tag="VNN25_09730"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXH91848.1"
                     /translation="MQLAEARARVATPNAIPERIIPCLDVRDGRVVKGVNFVDIRDAG
                     DPVECAARYEAEGADELVILDISATDANRTTALETVHRVSESLFIPLTVGGGVRTIED
                     FRALLRAGADRVAINTAALRNPQLIADCAREFGVQAVVLSCDAKRVNDHFEAMVRSGK
                     ESSGVDALAWCRQAEELGTGEILLTSVDRDGTNAGYDIELLRAVTSVVRISVIASGGA
                     GSLEHFRDAIEAGGARAVLAASLFHDRRLAIGDVKRYLSKEGIPVR"
     gene            complement(1544..2158)
                     /locus_tag="VNN25_09735"
                     /pseudo
     CDS             complement(1544..2158)
                     /locus_tag="VNN25_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
     gene            complement(2149..2751)
                     /gene="hisH"
                     /locus_tag="VNN25_09740"
     CDS             complement(2149..2751)
                     /gene="hisH"
                     /locus_tag="VNN25_09740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXH91849.1"
                     /translation="MITTLIDSPVANVANIARALRTVGAELQVTRDPDAIASAEKLVL
                     PGVGSFIAAMRWLEENRIDSAIRGAVANGASLLGVCVGHQLLFDVSLEMGTTRGLGLV
                     RGEVHKFEGSLPVPQIGWNRVQTAEGGGSTLFEGVGNGVFYFVNSYAASNAPDEIARA
                     DYGGSFTAAVQRDRIFGVQFHPEKSSAAGLRVLRNFVSWT"
     gene            complement(2776..2910)
                     /locus_tag="VNN25_09745"
     CDS             complement(2776..2910)
                     /locus_tag="VNN25_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91850.1"
                     /translation="MHVDLIRGRNGHHAAESIFKAFAIALREAKSVVGTDIPSTKGVI
                     "
BASE COUNT          489 a          914 c          972 g          543 t
ORIGIN      
        1 gaagaagacg ttacttctcc ctcttcgcca actctccttc gacctcgctc cagtcgatgc
       61 cttcgtcgga catcaggacg gtgacgtgga agacgaggtc ggcgatctcg ctgatcatgc
      121 ggtcgcgggt ttcgctgagg gcggcgatga ccacttcggt ggcttcttct ccgatcttct
      181 tgacgatgcg ggcgcggcct ccgctgaaga gcttcgcggt gtaggagcct tcgggaccgt
      241 tctctcggcg gtcgcggaga acgttcatga gacggtcgag ggttcgcggc ggcgtgcctg
      301 cgaagcatga tcgggcgccg gtgtggcacg cggggccgtt cgggatcacg ctgactacga
      361 gggcgtcggc gtcgcaatct tctgtgatgg cgacgacgcg ctgcgtgttg ccggaggtcg
      421 cgcctttgtg ccacagctcg ttgcgcgagc ggctccagag ccacgtctcg ccgctctcgc
      481 gcgtgcgatc gagcgactcc tgattcatgt acgcgagcgt cagcacctcg ccggttcgag
      541 cgtcctggat gactgccggg atgaggccgt tggcgtcgaa tttcagatcg ttcatcgttg
      601 ctcccaggga gtggattagt gattagtcga ttaggtttag gaagatcccg accttctgat
      661 ccactaatct actaatcact aatccactgt ctcacctcac cggaatccct tccttggaca
      721 agtagcgttt cacatcccca attgccagcc gccggtcatg aaagagcgac gccgccagta
      781 ccgctcttgc gccgccggct tcgatggcgt cgcggaagtg ttcgagcgaa ccggcgccgc
      841 ccgaggcgat gacgctgatg cggacgacgc tggtcactgc gcgcaacaac tcgatgtcgt
      901 atccggcgtt cgttccgtcg cggtcgacgg acgtcagcag gatctcgccg gttcccagct
      961 cctcggcctg cctgcaccac gccaaggcat cgacgcccga cgattctttg ccgctgcgca
     1021 ccatcgcttc gaagtgatcg ttaacgcgtt tggcatcgca cgacagcacg acggcttgca
     1081 cgccgaactc gcgagcgcag tcggcgatga gctgcggatt gcgaagcgcg gcggtgttga
     1141 tggcgacgcg atcggcgcct gcgcgaagca gcgcgcgaaa gtcttcgatc gtccgtacgc
     1201 cgccgccgac cgtgaggggg atgaagagcg actcggagac acggtgtacg gtctccagcg
     1261 ccgtcgtgcg attggcgtcc gtcgccgaga tgtcgaggat cacgagctcg tcggcgcctt
     1321 ccgcttcgta gcgcgccgcg cattcgaccg gatcgccggc atcgcggata tcgacgaagt
     1381 tcacgccttt gacgacgcgt ccatcgcgaa cgtcgaggca gggaatgatg cgctccggga
     1441 tcgcgttcgg cgtcgccacg cgtgcccgcg cctcggccag ttgcatgcgg cgatcgagca
     1501 gcgcccgtcc gatgacgcac gcgccgacgc tgcggatgcg gctcagcgag acgacgtcgc
     1561 cgagggtgga gacgccgccg gaagcgatga tgcgcagctt cgtttgcagc gagcggtaga
     1621 gcgcgaacga tggcccgcgc atggcgccgt ctttgttgat gtcggtgatg agcgcttcgg
     1681 caattccgag cgcttcgaca cgcgcgagca cctcggccag cgtggcccgt gcgttgcttt
     1741 cccatccgtt gacgcggacg atgccgtcgc ggacatcgat cgcggcgatg acgcgctcgg
     1801 cgccgagagc atcgacgatc tcacgcagca cggcgggcga gtcggccgcg acggtgccga
     1861 tcacgacctt cgctgcgccg gcgtcgatcc atgcgcgcgc gtcggcgagc gagcgaatgc
     1921 cgccgccgac ctgaacgcgc aagtcgcgct tcgcgagccg tcgtacaacg tccaactcga
     1981 tcggacgtcc gctgcgggac gcttcgaggt cgacgaggtg caggcgtgat ccgcgcggca
     2041 cgtcgagcga atcgaggacg gactccgggt cgccgtatgc cgtgaccttg gcaaagtcgc
     2101 cgtgcagcag gcggacgact tgcccgccga ggaggtcgag ggaggggatc acgtccatga
     2161 gacgaagttc ctcaggacgc gcaggcctgc cgccgatgat ttctcaggat gaaactgcac
     2221 gccgaagatt cgatcgcgct gcactgccgc ggtgaacgaa ccgccgtaat cggcgcgcgc
     2281 aatctcgtcc ggcgcgttcg aagcggcgta cgaattgacg aagtaaaaca caccgttccc
     2341 gacgccctcg aaaagcgtgg atcctccgcc ctctgcggtc tgaacgcgat tccatccgat
     2401 ctgcggaacg ggaagcgagc cttcgaattt atgcacttcg cctcgcacca ggccgagtcc
     2461 gcgcgtggta cccatttcga gggagacgtc gaagagaagc tggtgcccga cgcagacacc
     2521 gagcagtgat gcgccgtttg cgacggctcc gcgaatcgcg ctgtcgatgc ggttctcttc
     2581 cagccagcgc atcgccgcga tgaacgaacc gacgccggga aggacgagtt tctcggcgct
     2641 ggcgatggcg tcgggatcgc gcgttacttg gagctccgcg ccgaccgtgc ggagggcacg
     2701 cgcgatgttg gcgacgttgg cgacggggga gtcgatgagg gtcgtgatca tgagttgttg
     2761 ttagcgcgac gcgacctaaa tgactccctt cgtgcttggg atgtccgtcc ccaccacgct
     2821 ctttgcttcg cgcaatgcga tcgcgaacgc tttgaagatc gactcggcgg cgtggtgtcc
     2881 gttgcggccg cggatgagat cgacgtggat gttgcatg
//
LOCUS       DATLVW010000315         2380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_302257, whole genome shotgun sequence.
ACCESSION   DATLVW010000315 DATLVW010000000
VERSION     DATLVW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302257"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VNN25_09750"
     CDS             complement(<1..806)
                     /locus_tag="VNN25_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXH91851.1"
                     /translation="MNEPRITPELIASHGLSPDEYARIEQILGRAPNYTELGIFSVMW
                     SEHCSYKSSRVHLRKFPTTGPRVIQGPGENAGVIDIGDGWVAAFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIACLDSLRFGELDAPRMKYLVDGVVRGISGYGNCIGI
                     PTVGGETSFHSSYNGNILVNVFALGVTTRDRIFNGTASGVGNPVIYVGSKTGRDGIHG
                     ATMASAEFDDKSAEKRPAVQVGDPFMEKLLLEACLEVMKTDTLIGIQDMGA"
     gene            complement(873..1355)
                     /locus_tag="VNN25_09755"
     CDS             complement(873..1355)
                     /locus_tag="VNN25_09755"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXH91852.1"
                     /translation="MKKTIVVLVAALSIAAFAFAQEPEHPGMMMKHRLAMGMGPGSEE
                     IASALNLSTDQKVQWDAIHSQLEASVKPLLDQHRAAEQQLQAAAEATNPDATAVGRAF
                     LAMRAIDKQIQATHESTHAKLTAILTPDQKAKFDAMHAKMEHGEPGPMMMRHHDGARE
                     "
     gene            1495..>2380
                     /locus_tag="VNN25_09760"
     CDS             1495..>2380
                     /locus_tag="VNN25_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91853.1"
                     /translation="MKRSRNAIAALISLAVLGAALVVLGALQYRWIGEMADAERQRMR
                     AGIEFAAHHYGDDFDHELTRMFFAFQMPLPEATPDHLLRVYDEWSASSRDPRIVKAIY
                     FVPNGVDKIELVDPATRSLRPVAWPAALADLRPMIAAEFAGGRPVPPLIPRVTALVVP
                     CGGMERMKMMAIHRAVMGMAHIEMPRGEPGPPGGGPVCPAYTIVELDRDYIARVLLPD
                     LTHRYFDTPEGRQYEVAIVATDNGDLLYRSDDSTAPFRADFTLPIFSVRALRREPFDS
                     PVLLREPQMHPAVWQLQVR"
BASE COUNT          387 a          752 c          768 g          473 t
ORIGIN      
        1 gcgcccatgt cctggatgcc gatgagcgta tccgtcttca tcacctcgag gcacgcctcg
       61 aggaggagct tctccatgaa cggatcgccg acctgcacgg ccggacgctt ctccgccgat
      121 ttgtcatcga actccgccga cgccatcgtc gcgccgtgaa tgccatcgcg tcccgtcttc
      181 gatccgacat agatcaccgg attgccgacg ccgcttgctg ttccgttgaa gatccggtcg
      241 cgggtcgtga cgccgagggc gaagacgttg acgaggatgt tgccgttgta ggacgagtgg
      301 aacgacgtct cgccgccgac cgtgggaatg ccgatgcagt tgccgtagcc gctgatgcct
      361 cgtacgacgc cgtcgacgag atacttcatg cgcggcgcat ccagctcacc gaaccgcagc
      421 gagtcgaggc aggcgatcgg ccgcgcgccc atcgtgaaaa tatcgcgcaa gatcccgccg
      481 acgccggtgg ccgcaccctg gtatggctcg atgaacgacg gatggttgtg cgactccatc
      541 ttgaacgctg ccacccagcc gtcgccgata tcgatcacgc ccgcgttttc gccgggaccc
      601 tgaatcacgc gcgggccggt cgtcggaaac ttgcgaaggt gcacgcgcga tgatttgtac
      661 gagcagtgct ccgaccacat cacgctgaag atgccgagct ccgtgtagtt gggcgcgcgg
      721 ccgaggattt gctcgatgcg tgcgtactcg tcggggctga gtccgtgcga ggcgatgagc
      781 tcgggagtga tgcgcggctc gttcatgccg ctgattctgt caaaaaaagg gccgccgagg
      841 cggcggccct gcggattgct gtcaggatcg cgttattcgc gggcgccgtc gtgatgacgc
      901 atcatcatcg gtccgggctc gccgtgctcc atcttggcgt gcatggcgtc gaacttcgcc
      961 ttctgatcgg gcgtcaggat cgcggtgagc ttcgcgtggg tcgattcatg cgtcgcctga
     1021 atctgtttgt cgatggcgcg catcgccagg aacgcccgtc cgactgccgt ggcatccgga
     1081 ttcgtggctt ctgcggcagc ctgcaactgc tgttcggcag cgcgatgctg gtcgaggagc
     1141 ggcttcacgg acgcttcgag ctgcgagtgg atggcgtccc attgcacctt ctgatcggtt
     1201 gagaggttga gtgcggaagc gatctcttcg ctacccgggc ccattcccat ggcgaggcga
     1261 tgcttcatca tcatgccggg atgttccggc tcctgggcga aggcgaaggc ggcgattgag
     1321 agtgctgcga cgaggacaac gatggtcttt ttcattgtgt gtgtttcctt tcgctgggca
     1381 atgtacgcat cgtacgcctc ggagtgatca acagcacgca agctgtttgt aagaaacgta
     1441 aaacccgctt ccagccgcca tttccgcgca aatacggatg ctagactcga ccgcatgaag
     1501 cgcagccgca atgccatcgc cgcgctgatc tcgctggcgg tcctgggcgc ggcgctcgtc
     1561 gtgctcggag cgctgcagta tcgctggatc ggtgagatgg ccgatgccga gcgccagcga
     1621 atgcgcgcgg gcatcgaatt cgccgcgcat cactacggcg acgacttcga tcacgagctc
     1681 acccggatgt tcttcgcgtt ccagatgccg cttccggaag cgactccaga ccatctgctg
     1741 cgcgtctatg acgagtggtc ggcgtcgtcg cgcgatccgc gcatcgtgaa agccatctac
     1801 ttcgtgccaa acggagtcga caaaatcgag ctcgtcgatc cggcgacgcg cagccttcgt
     1861 cccgtcgcgt ggcctgcggc gctcgcggac ctgcggccga tgatcgccgc cgagtttgcc
     1921 ggtggccgtc ctgtgccgcc gctgattccg cgcgtgaccg cgctcgtcgt gccgtgcggc
     1981 gggatggaac gcatgaagat gatggccatt catcgcgccg tgatgggaat ggcgcacatc
     2041 gagatgccgc gcggggagcc cgggccgccc ggcggaggtc ccgtgtgtcc ggcctacacg
     2101 atcgtcgagc tcgatcgcga ctacattgcg cgcgtcctgc ttcccgacct cacgcaccgc
     2161 tacttcgaca cgccggaagg gcgccaatac gaagtagcca ttgtcgcgac ggacaacggc
     2221 gatctcctct atcggtcgga cgattcgacg gcgccgttcc gcgccgattt cacgctcccg
     2281 atcttcagcg tgcgcgcgct gcggcgtgag cccttcgata gccccgtgct gctacgtgag
     2341 ccgcagatgc atccggcggt ctggcagctt caggtgcggt
//
LOCUS       DATLVW010000316         5788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_383174, whole genome shotgun sequence.
ACCESSION   DATLVW010000316 DATLVW010000000
VERSION     DATLVW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383174"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VNN25_09765"
     CDS             complement(<1..538)
                     /locus_tag="VNN25_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXH91854.1"
                     /translation="MKSIRIDVDGAVGTITIDRRERFNSLDVEMARDLRKAGLQLARD
                     NAIRAVVLRGNGGVFCSGADLKYIRGGGDPKGLGYLQPNSASPAPPLETVSHAEPRRS
                     GENRYGDVFKQILEYIHSTISEIRRAPKPFIAAVDGIAAAGGFGLAMSCDLVIASDRA
                     MFEWAYAKTGLTGAESSTF"
     gene            complement(535..1104)
                     /locus_tag="VNN25_09770"
     CDS             complement(535..1104)
                     /locus_tag="VNN25_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HXH91855.1"
                     /translation="MLNVAVLCSKRAPGLEALRERVHVSCVLDRQLPRRADRESFDAE
                     TASLLLPYDVDAVILLSYLYILTDPMLETFPGRILNVHDSDLALRNDDGGPRYPGLHA
                     TCDAINAGEHETRSSVHLVTKHPDAGPVLARSNAFPVAPFVSVAAVRGEQDIVRAYAY
                     AQREWMMRSTWGALASEALGRLIPQKAAA"
     gene            complement(1098..2150)
                     /gene="hypE"
                     /locus_tag="VNN25_09775"
     CDS             complement(1098..2150)
                     /gene="hypE"
                     /locus_tag="VNN25_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HXH91856.1"
                     /translation="MTAILTAPGGRVTLKQGGGGRAMRALIEKLFVRDFATMPLHNPD
                     FSVGVGAMDDGAAIRIGDEWLVITTDSHVIHPIFFPGGDIGRLSICGTVNDLAMMGAT
                     NVLGLTCSVILEEGFALDSLEAIQRSMIAACREAGAPVVTGDTKVMGKRELDGIVINT
                     CGIGLTRTLVRDNGLSLGDRIIVTGSIAEHGLAVMAKRHEIGIEGDLISDVAPLNDLV
                     ASVLAGAPGTVTAMKDPTRGGVSSALHEMASKSGVGILLDERAIPVTAAVRAVGELLG
                     IDPLHVANEGKALIGVRADAADRVLDILRAHPLGRNAAVIGTCVAERPGGVILNTGFG
                     KRLLAEPEGEPLPRIC"
     gene            complement(2147..3265)
                     /gene="hypD"
                     /locus_tag="VNN25_09780"
     CDS             complement(2147..3265)
                     /gene="hypD"
                     /locus_tag="VNN25_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HXH91857.1"
                     /translation="MTNELQFRDPALARSLAATLARLVDEIGRPVAVMHVCGSHEQAI
                     ARFGLRSLFPKALDVIMGPGCPVCVTDVPEVDEGVVLARQGVRIVTFGDMLRVPGTVQ
                     SLADAQASGANIEVIYSPRQAIEIARETDEEVVLFATGFETTAVATAAVILDNPPKNF
                     SVLSAHKYIPPVMEIVAEMPGSRVEGFLAAGHAAAITGSAVFEPFVERHQVPVVVAGF
                     EPMDILAGLVELLELIRNNTPRVANMFPRCVSAEGNIPAQKQLWRVFKPVGGKWRGIA
                     HVPNGNLRLRDEFAHVDARKRFTIDLPSLWDYAPPALAGQCICGDIMAGIRNPRDCRL
                     FNRECVPENPVGACMVSNEGTCKIWHLYGGVPDLEATA"
     gene            complement(3262..3543)
                     /locus_tag="VNN25_09785"
     CDS             complement(3262..3543)
                     /locus_tag="VNN25_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HXH91858.1"
                     /translation="MCLGVPGRVISIHDYTAVVDFWGVRKDVRLDIVDEPVVPGDYIL
                     NHVGFAIRRIAPESVAETLELYEMLLRENAGGEDDLMALDVRAEIAGAS"
     gene            complement(3534..>5788)
                     /gene="hypF"
                     /locus_tag="VNN25_09790"
     CDS             complement(3534..>5788)
                     /gene="hypF"
                     /locus_tag="VNN25_09790"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXH91859.1"
                     /translation="QRAGVRGVVRNDSAGVSIDAFGTADALDQFVAALRGGTPPAARV
                     QSVQSAVIPFVEHSAFAITDSVASAERHVSIPTDLATCEDCVRELLDPADRRFRYPFI
                     NCTNCGPRYSIVLDAPYDRPKTSMAPFRMCAHCLAEYESPSNRRFHAQPNACPDCGPR
                     LTAMGSRHERIVTPDAIRFAARAIRAGMTVALKGLGGFQLACDATSSQAVQRLRDRKQ
                     REHKPLAVMVRDLAMAEELVELNDAERELLTSIERPIVLARSREDSALAQEVCGAGIV
                     IAGVFLPYTPLHHLLLRDAAIPLVMTSGNLADEPMITSNADALEQLRNVADVFLLHDR
                     EIVTRVDDSIATVIDGAPALLRRARGYVPRSIDTSINGSRTFSEPILACGAHLKNTFC
                     IASGSSAFLGPHIGDLESVETLRAYESAIERMKSFIGVSPRVLVHDLHPDYFSTRYAL
                     AQQGVRTIGVQHHHAHIASAMAEDRIEGPVIGIAYDGTGFGTDGTSWGGEILIADYED
                     YERFATFRPILLAGGDQAIRQPWRIALALLDDTYAGDAPLASLPLFRELSLQMITQVR
                     LMIDRNLNTTPARGVGRLFDAFGAIVLGLTDAHYEGEVAFRWNMVADENERGRYPIVI
                     HDGIRPWQIDPRPMVKAAVNDLLAGRSAGTISARFHNTLAAATAEIARAALISRGRMP
                     VVISGGCFQNRRLAESIIDTLRPSVRVVMNRNVPAGDGGIALGQAFIADARLRAEMSV
                     SVDEEELVCA"
BASE COUNT         1036 a         1827 c         1966 g          959 t
ORIGIN      
        1 tgaaggtcga gctttcggcg cccgtcaggc ctgtcttcgc gtaggcccac tcgaacatcg
       61 cgcgatcgga ggcgatgacg aggtcgcagg acatggccag gccgaatccg cccgcggctg
      121 cgatgccgtc gaccgcggcg atgaacggct tcggcgcgcg gcggatctcg gagatcgtgc
      181 tgtggatgta ttcgaggatc tgcttgaaga cgtcaccgta gcggttttct ccgcttctcc
      241 gcggctccgc gtgagaaacg gtttcaagcg gaggcgcggg ggacgcggag ttgggttgca
      301 ggtatccgag gcctttgggg tcgccgccgc cgcggatgta tttcaaatca gcgccgctgc
      361 agaagacgcc gccgttgccg cgcagcacca ccgcgcggat ggcgttgtcg cgcgcgagtt
      421 gcaggccggc tttgcgcaga tcgcgggcca tctcgacatc gagggagttg aagcgctcgc
      481 ggcgatcgat cgtgatggtc ccgacggcgc cgtcaacatc gatgcggatc gatttcacgc
      541 ggccgccttc tgcggaatca ggcgcccgag ggcctcgctg gcgagcgcgc cccaggtgct
      601 gcgcatcatc cactcgcgtt gcgcataggc atacgcgcga acgatgtcct gctcgccgcg
      661 caccgcggcc acgctcacga atggggcaac gggaaacgca ttgctgcgcg cgagcacggg
      721 gccggcgtcg ggatgcttcg tgacgagatg cacgctgctg cgcgtctcgt gctcaccggc
      781 gttgatggcg tcgcacgtgg cgtgcagccc cggataacgc ggaccgccgt cgtcattgcg
      841 cagcgcgaga tcagaatcgt gaacgttgag aatacgcccc ggaaacgtct ccagcatcgg
      901 atcggtgagg atgtagagat agctgagtag gatgacggcg tcgacgtcgt acggaaggag
      961 cagcgaggcg gtctcggcgt cgaacgattc gcggtcggcg cggcgcggaa gctggcggtc
     1021 aaggacgcag gagacatgga cgcgctctcg caaggcctcg aggcccggcg cgcgtttcga
     1081 gcagaggacg gcgacgttca acatatgcgt ggtaacggct cgccttccgg ctcagcgagc
     1141 agcctctttc cgaatccggt attgagaatg acgccgcccg gccgctcggc gacacatgtg
     1201 ccgatgactg cggcattgcg gccgagcggg tgcgcgcgaa gaatgtcgag aacgcgatcg
     1261 gcggcatcgg cacgaacgcc gatgagcgct tttccttcgt tggcgacgtg cagaggatcg
     1321 atgccgagta gctcgccgac agcgcgaacc gcggcagtga ccgggatggc gcgctcgtcg
     1381 agcaggatgc cgacgccgct cttcgacgcc atctcgtgca gcgcgctgga gacgccgccg
     1441 cgcgtcggat ctttcatcgc cgtcacggtg ccgggagctc cggcgagaac ggacgcgacg
     1501 aggtcattga gcggagcgac atcggaaatc agatcgcctt cgatcccgat ctcgtgccgc
     1561 ttcgccatca ccgccaggcc gtgctccgca atcgagccgg tgacgatgat gcgatcgccg
     1621 agagagaggc cgttgtcgcg cacgagcgtg cgtgtgagtc cgatgccgca agtgttgatg
     1681 acgatgccgt cgagctcgcg cttgcccatc accttcgtgt cgccggtgac gacgggagcg
     1741 ccggcctcgc ggcacgcggc gatcatcgat cgctgaatgg cttcgagcga atcgagcgcg
     1801 aaaccttctt cgagaatgac gctgcacgtc aatcccagaa cgttcgtggc gcccatcatg
     1861 gcaaggtcgt tcaccgtgcc gcagatggag agccggccga tgtcgccgcc gggaaagaag
     1921 atcggatgga tgacgtgcga gtcggtggtg atgacgagcc attcgtcgcc gatgcgaatg
     1981 gccgcgccgt cgtccatagc gccgacgccg acgctgaagt cgggattgtg cagcggcatc
     2041 gtggcgaaat cgcgcacgaa gagtttctcg atgagcgcgc gcatggcccg tccgccgccg
     2101 ccctgcttga gcgtgacgcg gccgccgggc gccgtgagga tcgcggtcat gcggtcgcct
     2161 ccagatcggg aacgccgccg tagagatgcc agatcttgca cgtgccttcg ttcgacacca
     2221 tgcaggcgcc cacagggttc tcgggaacac actcgcggtt gaagagacgg cagtcgcgcg
     2281 ggttgcggat gccggccatg atgtcgccgc aaatgcactg cccggccagc gccggtggcg
     2341 cgtagtccca cagcgaggga agatcgatcg tgaagcgttt gcgcgcgtcg acgtgtgcga
     2401 actcgtcgcg caaacgaagg ttgccgttcg gcacgtgcgc gatgccgcgc catttaccgc
     2461 cgaccggttt gaagacgcgc cacagttgct tctgcgccgg aatgttcccc tcggcagaca
     2521 cacaccgcgg aaacatgttc gccacgcgcg gggtattgtt gcggatcagc tcgagcagct
     2581 cgacgagccc ggccagaatg tccatcggct cgaatccggc cacgacgacc ggcacctggt
     2641 gccgctcgac gaacggttcg aagaccgccg agccggtgat cgccgcggcg tgaccggcgg
     2701 cgagaaatcc ttccacgcgc gatcccggca tctccgcgac gatctccatc accggcggga
     2761 tatatttgtg cgcggacaga acggagaagt tcttcggcgg attgtcgagg atcaccgcgg
     2821 ccgtggccac ggccgtcgtc tcgaatccgg tggcgaagag gacgacttcc tcatcggtct
     2881 cgcgcgcgat ctcgatcgcc tggcgcgggc tatagatgac ttcgatattc gcgccactgg
     2941 cctgcgcatc ggcgagcgat tgcacggtgc cgggcacgcg cagcatgtcg ccaaacgtga
     3001 cgatgcgaac accttggcga gcgagcacga cgccttcgtc gacctccgga acgtcggtga
     3061 cgcagaccgg gcaaccaggt cccatgatca catcgagcgc cttcggaaaa agcgatcgca
     3121 gaccgaagcg ggcgatggcc tgctcgtgac tgccgcagac gtgcatgacc gcgaccggcc
     3181 gcccgatctc atcgacaagg cgggccagcg tcgccgcgag cgagcgcgcc agcgccggat
     3241 cacggaactg cagctcgttc gtcacgaagc ccccgcgatt tcggcgcgga cgtcgagcgc
     3301 catcaggtcg tcctcgccgc cggcattctc gcgcagcagc atttcgtaga gctccagcgt
     3361 ctcggccacc gactcggggg caatgcggcg aatggcgaag ccgacatggt tgaggatgta
     3421 gtcgcccggc accactggct catcgacgat atcgagccgc acgtctttgc gaacgcccca
     3481 gaagtcgacg accgcggtgt agtcgtgaat cgaaatgacg cgtcccggta cacctaggca
     3541 catacgagct cctcttcatc gacgctcacc gacatttccg cgcgaagccg cgcatcggcg
     3601 atgaacgcct ggccaagcgc gatgccgccg tcgccagccg gaacgttgcg gttcatgacg
     3661 acgcgcacag acggacgaag ggtgtcgatg atcgactccg ccaatcgtct gttttgaaag
     3721 cagccgccgc tgatcacgac cggcatgcgc ccgcggctga tgagcgcggc gcgcgcgatc
     3781 tccgcggtgg ccgcagccag cgtgttgtgg aagcgcgcgg agatcgtgcc ggcggaacga
     3841 ccggccagca gatcgttgac cgccgctttc accatcggcc gcggatcgat ctgccacggt
     3901 ctgatgccgt cgtggatgac gatcggataa cgtccgcgct cgttttcgtc cgcgaccatg
     3961 ttccagcgga aggcaacttc gccttcgtag tgcgcgtcag tcagtccgag gacgatcgct
     4021 ccgaaagcat cgaacaatcg tccgacgccg cgggcgggag tcgtgttcag gttgcgatcg
     4081 atcatcagcc gcacctgcgt gatcatctgc agcgagagct cgcggaagag aggaagcgat
     4141 gccagtggcg cgtctccggc atacgtgtcg tcgagcagcg ccagcgcgat gcgccacggc
     4201 tggcggatgg cctgatcgcc gcccgcgagc agaatgggac ggaacgttgc gaagcgctcg
     4261 tagtcttcgt aatcggcaat gagaatctcg ccgccccacg acgtaccgtc ggtgccgaat
     4321 cccgttccgt cgtacgcaat cccgatcacc ggcccttcga tccgatcctc ggccattgcg
     4381 ctggcgatgt gcgcgtgatg atgctgcacg ccgatcgtgc gcacgccttg ctgcgcgagc
     4441 gcgtaccgcg tggagaagta gtccggatga aggtcgtgca cgagcacgcg cggcgacacg
     4501 ccgatgaacg atttcatccg ctcgatggcc gactcgtacg cgcgcagcgt ctcgacgctc
     4561 tcgaggtcgc cgatgtgcgg cccgaggaat gcgctcgatc cggaagcgat gcagaaggtg
     4621 ttcttgagat gcgcgccgca agcaagaatc ggctcggaga acgtgcggct gccattgatt
     4681 gacgtatcga tcgatcgtgg cacatagcca cgggcacggc ggagaagcgc cggcgcgccg
     4741 tcgatgactg tggcgatgga gtcgtcgacg cgcgtaacga tctcgcggtc gtgcagaagg
     4801 aagacgtcgg cgacgttgcg aagctgttcc agcgcgtccg cgttggaagt gatcatcggc
     4861 tcatcagcga ggttccccga ggtcatcacg agggggatgg cggcatcgcg aagcaggaga
     4921 tgatgcagcg gcgtgtacgg aaggaagacg cctgcgatga cgattcccgc gccgcacact
     4981 tcctgcgcga gcgctgagtc ctcgcggctg cgcgcaagca cgatcggccg ttcgatcgac
     5041 gtgagcagtt ccctctccgc gtcgttcaac tccaccaact cctcggccat cgccagatcg
     5101 cgcaccatca ccgccagcgg tttgtgctcg cgctgtttgc ggtcgcgcag ccgctgcacg
     5161 gcctgcgagg aggtggcatc gcaggcgagc tggaatccgc cgagtccttt gagcgcgacg
     5221 gtcatgccgg cgcggatggc gcgggcggcg aagcggatcg cgtcgggcgt cacgatccgc
     5281 tcgtgccggc ttcccatggc cgtcagccgc ggcccgcaat cgggacaggc gttcggttgc
     5341 gcatggaagc gccggttgga gggagattcg tattcagcga gacagtgcgc gcacatgcgg
     5401 aacggcgcca tcgacgtctt cggccggtcg tacggcgcgt cgaggacgat agagtagcgc
     5461 ggcccgcagt tcgtgcagtt gatgaacgga tagcggaatc gccggtccgc cggatcgagc
     5521 aactcacgca cgcagtcttc gcacgtggca agatcggtgg gaatcgagac atgacgctcc
     5581 gcgctcgcaa cactgtcggt tattgcgaac gcggagtgct caacgaatgg gatcaccgcg
     5641 gactgtacgg actgcacgcg cgcggcggga ggagtacctc cgcgcaaagc agcaacgaat
     5701 tggtcaagag cgtctgcagt gccgaacgcg tcgatcgaca ccccagccga gtcgttgcga
     5761 acgaccccgc ggaccccggc gcgctgcg
//
LOCUS       DATLVW010000317         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_262052, whole genome shotgun sequence.
ACCESSION   DATLVW010000317 DATLVW010000000
VERSION     DATLVW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262052"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VNN25_09795"
     CDS             complement(<1..642)
                     /locus_tag="VNN25_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH91860.1"
                     /translation="MFKRILLTALLTIAAAAANASTLDEPFDRTFDVRPGAVFSLSNT
                     NGHINIRAWDQPRIHIHAVKHVESRDADTARSAMKALTLAVSQPNGGLRIDTKYPKSN
                     DGILEWIAGTSVNLSVTYEVVVPRTMNLQIENTNGGVEASDVSGSHRVETTNGHIRLA
                     RCAGDLDAETTNGGIEAELTSVTAGHVVRLETTNGAIRARLPRKLAASLDASTT"
     gene            744..2972
                     /locus_tag="VNN25_09800"
     CDS             744..2972
                     /locus_tag="VNN25_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH91861.1"
                     /translation="MKRGVAAGLLFATLAAILGVLLLRMRGPAPLPETAPASSFSATR
                     AIAFEREIIGTASHPVGTAAHDAVRERLAARLRALGYDVTIQRTFACSPYVTCAPVAN
                     VIARTPGDARPDALVLAAHYDSVPAGPGASDDGQGVAALVEIARALRNEHFRNPIVYL
                     ITDAEEVALLGAEGFVADPNLLRSAAGVINIEARGTDGPSFLFETSRQNEWIARTIAS
                     ALPRPATSSLYYDIYELLPNDTDLTVFKRVGLTGIGFANIGQPVHYHSPLDNLDNVTL
                     STLQHHGDHILAMARAFAATDLRQTSRGNAVWFDVLSFFVVWWPQRYSLGIAILSMIV
                     LLAATFLMARDGRTKSRAITLGVLSFFLTIIAALAVGFAGNWLAGLRAGGAVWVAYPG
                     TAIATAWLIGIGAAIVIAQRFIKSAGVDGLFLGHAICWSAIAIALAIVLPGGSYLTIV
                     PAVALALCALLRATVKMSEGVSAILCAAVAAILIFPLGLALYVALGKPILPFIALLIA
                     LVATTFTPFIAGMPPLKRPILTALAVALIACIVIANLVPAYTADSPRHINIEYVDDGA
                     APHWQADALLRAIAQAANFDATPKQIADWTTPPAKALIAPAPSLGLAPAELQVIGDEH
                     QNGRHLTLHLHSARNAPRIALIFHAPSFASMRVNGIVPPPVTRRRGGLAEGWHRVSVR
                     ATEATIDLYLTRNEPIDAVIIDGSYGLPPEGAALTHARDTSLAIPVQEGDLTTTIRRK
                     RL"
     gene            3115..>3343
                     /locus_tag="VNN25_09805"
     CDS             3115..>3343
                     /locus_tag="VNN25_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91862.1"
                     /translation="MSSGVRRGTAMMLALLSVSALADTRWLPSPAILREHMCASQSVV
                     VGRLTDVTVQQGAQARSGSGRIEVDHVIIRVG"
BASE COUNT          544 a         1187 c          988 g          624 t
ORIGIN      
        1 ggtcgtggat gcgtccaggc tcgcggccag tttccgcggc aggcgggcgc ggatcgcacc
       61 attcgtcgtc tccaggcgaa cgacgtgccc ggccgtgacc gacgtcagct ccgcttcgat
      121 cccgccgttc gtcgtctccg catcgagatc tcccgcgcag cgcgccagcc ggatgtgacc
      181 gttggtcgtc tcgactcgat gcgaaccgct gacgtcgctc gcttcgactc ctccgttcgt
      241 attctcgatc tgcagattca tcgtgcgtgg caccacgacc tcgtaagtca ccgagagatt
      301 cacgctcgtg ccggcgatcc attcgagaat gccgtcgttc gacttcggat atttcgtgtc
      361 gatgcgcaag ccgccgtttg gctgcgacac cgccagagtc agtgccttca tcgcactgcg
      421 cgcggtatcg gcatcacggc tttcgacgtg cttcacggcg tgaatgtgaa tcctcggctg
      481 atcccacgcg cgaatgttga tgtgcccatt cgtattcgac agcgagaaga ccgcgcccgg
      541 ccggacatcg aacgtgcggt cgaacggctc gtccagcgtc gatgcattcg cagccgcggc
      601 agcgatggtg aggagggcgg tgagcaggat gcgcttgaac atgtcttttg tgtcctttga
      661 tggctagtgg atcagtggat ctctaatctc tgaacgctca ttacgtcccg catccccgca
      721 ggttgcagct acaatccgcg atcgtgaaac gtggcgtcgc ggcaggactc ctcttcgcga
      781 cgctcgcggc gattctgggc gtgctgcttc tgcggatgcg cggcccggcg ccgctgcccg
      841 aaaccgcgcc ggcatcctcg ttctcggcaa cacgcgccat cgccttcgag cgcgagatca
      901 tcggcaccgc atcgcatccg gttggaaccg cggctcacga tgccgtccgt gaacgtctgg
      961 ccgcccgcct tcgcgccctc ggctacgacg tcaccatcca gcgcacgttc gcatgcagcc
     1021 cgtacgtcac ctgtgcgccg gtcgcgaacg tcatcgcccg cactcccggc gacgcgcggc
     1081 ctgacgcgct cgtcctggcc gcgcactacg actccgtgcc cgccggaccc ggtgcttccg
     1141 acgatggaca gggcgtagcg gcgctcgtcg agatcgcccg cgcgctgcgc aacgagcact
     1201 tccgcaatcc gatcgtctac ctcatcacgg acgccgaaga ggtagcgctg ctgggagcgg
     1261 aaggcttcgt tgccgatccg aatctgctgc gcagcgcggc gggagtcatc aacatcgagg
     1321 cgcgcggaac ggatggaccg agcttcctct tcgagaccag ccggcagaac gagtggatcg
     1381 cgcgcacgat cgcgagcgcg ctgccgcggc cggccacgag ctcgctttac tacgacatct
     1441 acgagcttct gccgaacgac accgacctca ccgtcttcaa acgcgtcggg ctgacgggaa
     1501 tcggcttcgc caacatcggc cagccggttc actaccattc gccgctcgac aatctcgaca
     1561 acgtcaccct gtcgacgttg cagcatcacg gcgatcacat cctggcgatg gcgcgcgcgt
     1621 tcgccgccac cgatctgcgg cagacgagcc gcggcaacgc cgtctggttc gacgtcctct
     1681 ccttcttcgt cgtctggtgg ccacagcgct actccctcgg catcgccatc ctttcgatga
     1741 tcgttctgct cgccgccacg ttcttgatgg cgcgcgacgg cagaacgaaa tcgcgcgcga
     1801 tcaccctcgg cgtcctctcg ttcttcctga cgatcatcgc agcactggcg gttggcttcg
     1861 ccggcaactg gctggcggga ttgcgcgcag gcggtgccgt gtgggtcgcc tatcccggca
     1921 ccgcgatcgc gaccgcgtgg ctgatcggaa tcggcgccgc catcgtcatc gcgcagcgct
     1981 tcatcaagtc cgccggcgtc gacggcctct tcctcggaca cgcgatctgc tggtccgcga
     2041 tcgccatcgc gctcgccatc gtgcttcccg gcgggagcta tctcacgatc gtgcccgcgg
     2101 tcgcgttggc gctctgcgcg ctgcttcgcg caaccgtgaa gatgagcgaa ggagtgtccg
     2161 cgattctttg cgcggccgtc gcagcgatcc tgatcttccc gctcggtctg gcgttgtacg
     2221 tcgccctcgg caaaccgatc ctgccattta tcgcgttgct gatcgcgctc gtcgccacga
     2281 ccttcacgcc gttcattgcc ggcatgccgc cgctgaagcg cccgatcctc acggcgctcg
     2341 ctgtcgcgtt gatcgcgtgc atcgtcatcg ccaacctcgt cccggcctat acggccgatt
     2401 ctccgcgcca catcaacatc gaatacgtcg acgacggcgc cgcgccgcac tggcaagccg
     2461 acgcattgct ccgcgcaatc gcgcaggcag ccaacttcga tgcgacgccc aaacagatcg
     2521 ccgactggac cacgccgccc gcgaaggcac tcatcgcccc cgcacccagc ctcggtctgg
     2581 caccagcgga gttgcaggtc atcggcgacg aacaccagaa cggccgccac ctgacgttgc
     2641 atcttcactc cgcccgcaac gccccccgca tcgcactcat cttccacgcc ccatcattcg
     2701 catccatgcg cgtcaacgga atcgtccctc cgccggtcac acgccggcga ggcgggctgg
     2761 ccgaaggatg gcatcgcgtc agcgtccgcg ccaccgaagc aacgatcgat ctctacctga
     2821 ctcgcaacga accgatcgac gccgtcatca tcgacggctc ctacggcctc ccaccagaag
     2881 gcgcggcact cacccacgcg cgcgacacat cactcgccat tccagtacag gaaggcgacc
     2941 tcacgaccac aatccggcgc aagcgattgt gacccggtct ctcttcgcgt cttcgcgtga
     3001 cagctacttg gaagacgatc acgcgaagcc gcgaagtcgc gaagaacttc acgcggtttg
     3061 gcggcaggcg gttgtgaaac gaaaatagcc ggtctacagt gaatggtgat gacgatgagc
     3121 tcgggagtca ggcgtgggac ggcgatgatg ctcgcgctcc tttcggtctc cgcgcttgca
     3181 gacacgcggt ggcttccatc cccagcgatc cttcgggagc acatgtgcgc ctcgcagtcg
     3241 gtcgttgttg gccgcctcac cgacgtgacg gtgcagcagg gtgctcaggc caggtcgggg
     3301 tccggtcgca tcgaagtgga tcacgtcatc atccgggttg gcc
//
LOCUS       DATLVW010000318         7688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_548173, whole genome shotgun sequence.
ACCESSION   DATLVW010000318 DATLVW010000000
VERSION     DATLVW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548173"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..512)
                     /locus_tag="VNN25_09810"
     CDS             complement(231..512)
                     /locus_tag="VNN25_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91863.1"
                     /translation="MITPRITYLASFSLIVILGASIPATMWFEKEIGETGTHGPVAIV
                     AALPFISIFAAGAVILAVLVLRARMRMLPVAAGLLPFLILIGCGVLLFS"
     gene            complement(538..1302)
                     /locus_tag="VNN25_09815"
     CDS             complement(538..1302)
                     /locus_tag="VNN25_09815"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HXH91864.1"
                     /translation="MRLKVLGAYGASDAEHNLTGYLIDDWFAVDAGTLTSKLSFAQQA
                     RIQAVFITHTHADHIRDLPHLIHNRFSQTAGPLTVFAAKDVMELLISNVFNGIVWPDF
                     STLISPLTNKPAVIYRSLTPGKKVVFEGVGMTAVPVHHQIPAAGVIVEMNGQAIAFTG
                     DTGPTSEIWKRTNKTPGVVAVVTEASFPNDQQQLADDTAHLSPNTFGEELKKITVDAP
                     VYASHRKIPYERDIESQIRNLRDRRARVLLEKNYTL"
     gene            1448..5875
                     /locus_tag="VNN25_09820"
     CDS             1448..5875
                     /locus_tag="VNN25_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HXH91865.1"
                     /translation="MAPFHPAVRDWFANVFRTPTRAQELGWPAIARGDWTLIFAPTGS
                     GKTLTAFLWALDRLMFAERPEKDRRCRVVYISPLKALAVDVERNLRAPLTGIANLAAE
                     RSESFVVPSIAIRTGDTPQNERARFLRDPADILITTPESIYLMLTSNARDVLRGVETI
                     IIDEVHALVPNKRGAHLALSLERLQRVADKVPQRIGLSATQNPLEEVARYLGGFGGER
                     EVTIIDTRERKQLTLHVEVPVEEMAKLNESSRDDIPSGPAGKGVRASIWPAIHPRLLE
                     LIRAHRSTLIFVNSRRLAERLAGAINELAGEMLVQAHHGSIARAQRIEIEDNLKSGRL
                     PALVATSSLELGIDMGAIDLVIQIEAPPSIASGMQRIGRASHQVGTVSEGIIFPKFRG
                     DLLACAAVTEAMHRGAIEPTRYPRNPLDVLAQQVVAMIATDSYHADDLFALVRQAAPF
                     AELSRTSFDGVLDMLSGLYPSDEFAELRPRITWDRVTKILTPRPNAKRVAITSGGTIP
                     DRGLFGVFLAGGEKVQSARVGELDEEMVFESRVGETFLLGASTWRIEEITHDRVLVTP
                     APGEPGKMPFWKADAAERTVPFGEAIGALVREIGLMQPAAAIKRLTTKHDLGELAATN
                     FIQYLDDQQAATGAVPDDRTIVIERVLDDLGDWRVCVLTPFGGRIHAPWAMAVTAKVR
                     NELQIDVETMWSDDGFIVRFPDTDAPPRAELLIPDADEIEQLVVRQLGSSSMFAAKFR
                     EAAARALLLPRFRPDRRQPLWQQRKRAYDLLQVASRFGSFPIILETYREVLRDVFDVP
                     ALVATMKKIQQRTIRVVVADTEKPSPFAGSLLFRYVANFIYDGDAPLAERRAQALAID
                     QAQLRELLGEPELRELLDADALNALEIELQHLDERHKAKSLDALHDLFLRLGDLSFEE
                     VVARSLLTVGPSSTPDGATIESQVKAAIRELVKTRRIIEVGVAREKRFIAIEDASRYR
                     DALGAPLPPGLADRYLQPVADPTGDLVLRYARTHGPFTPHDVAHRYGLGVAVVTNSLE
                     RFVERGRLIEGEFRPGGTQREWCDNDVLSAIRRRSLAKLRKEVEPVETKAYVRLINHW
                     QFVTKKRRGLEGLLEVIEALQGAALPASIFESEILAARIDGYKPSDLDTLSAAGEIIW
                     TGVEPLGYRDGRIALYLTDHLPLLHSPVATDASELEQQIIDHLRASGASFFAEVQTAL
                     GGFANDVVDALWALVWRGVITNDTFHALRAYTRPKASPRTTGFRSRRVAPPSTQGRWS
                     LIQPPRGTATERASAIAHQLLARYGIVVREAAAVESIPGGFSAIYPVLKGMEESGRTR
                     RGYFVAGFGATQFGSAGALDLLRSYRDEPDEPETVMLAATDPANPYGSLLKWPVVGLM
                     RVPGANVILVNGMLGAYIGRGEKQLSVFLPENEPMRGVVAREIAKTLASLVTNGVRRA
                     MLIAEVNDAPAAKSDLAPYLAEAGFVPTAMGYQMRALRI"
     gene            5889..6698
                     /locus_tag="VNN25_09825"
     CDS             5889..6698
                     /locus_tag="VNN25_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXH91866.1"
                     /translation="MPEGDSIYRAAQALHRALAGKTVTSFETVLPKLARIDEDAPLAG
                     RTIERVISAGKNLIIEFSGDLHLHTHMKMNGSWHIYRPGERWQRPHRDMRIVIGTADF
                     VAVAFTVPVAEFHTSRSLARDENLRRIGPDLLGATFDRAEALRRIRAHPNEEIANVLL
                     NQRVIAGIGNIWKSETLFDAGIDPFTHVSDLDEIQLDRIISSALKLLRRSAEGRAEFA
                     VYSRGGDPCRKCRTPIRYRKQGEDARGTYWCPVCQRSAGVSPAAGGRPARR"
     gene            6829..>7688
                     /locus_tag="VNN25_09830"
     CDS             6829..>7688
                     /locus_tag="VNN25_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXH91867.1"
                     /translation="MARDLPIGNGSLMVNFDHEYNIRDVYYPHVGQDNQTNGDVSFFG
                     IWCDGKFAWIGDPSWVKRLDYDGDTLVTRMEAHSDSLQLDMTINDAIDFDRDVFIRRV
                     ELRNLADKKRTVRLFSHFDPHLSGNAIGDTIFFDPEFNGFVAYKAQRYVLINAASDDQ
                     FGFKAFAIGTKEREGYQGTWRDAEDGVFEPNKAIAQGSVDCTGMIEITLLPGAVSTAY
                     VWWVFGQSYKDVVALDTFVRERGAASLLDRTRDYWKLWVKNDVDISRLPEAVQRLYRR
                     SLLIIRTQVD"
BASE COUNT         1398 a         2554 c         2394 g         1342 t
ORIGIN      
        1 ccggatgcga aggtggcagg ccagatccaa ccaccgaggc gggacgcccc gatggccgcg
       61 ggcgagacgc ccacactcct catcgctgcg aggaaagccc gtcacaagga gtgcggtcgt
      121 ctcgaccgcg ggcagtcggg cgtcctcgcc cgagtgcggt ccgggcggcg gtcgaagttc
      181 gttgacgttc tcacctgcgt tgaaacagct gtgcggtcac cgagttgtcg tcacgagaaa
      241 agaagcacac cgcaaccgat caggatgagg aacggcaaaa ggccggcagc aactggaagc
      301 attctcattc gtgctcgcag aacgaggaca gccaggatca cagcgccggc agcgaagatc
      361 gagatgaaag gtagcgcggc aacgatggcg accggtccat gcgtgccggt ctcacctatc
      421 tccttctcga accacatcgt ggccggaatg cttgcgccaa gaatcacgat gagcgagaac
      481 gatgcgaggt acgtgatcct tggggtgatc aagatgaaac ctcgaaccca tatcccccta
      541 caacgtgtag ttcttctcca gcagcaccct cgcccttcga tcccgcagat tccggatctg
      601 cgactcgatg tcgcgctcgt atgggatctt gcggtggctg gcgtagacgg gggcgtcgac
      661 cgtgatcttc ttcagctctt cgccgaaggt gttgggggag aggtgggcgg tgtcgtcggc
      721 aagctgttgc tgatcgttcg ggaacgacgc ctcggtcacc acggcaacga cgccgggcgt
      781 cttgttcgtc cgcttccaga tttccgaggt ggggccggtg tcgccggtga aggcgatggc
      841 ctggccgttc atctccacga tcacgccggc ggcggggatc tggtgatgga cggggacggc
      901 ggtcatgccc acgccttcga acacaacctt cttgcccggc gtcagggaac ggtagatgac
      961 cgccggcttg ttcgtcagcg gggagatgag cgtcgagaaa tcgggccaga cgattccgtt
     1021 gaaaacgttc gagatcagca gctccatcac gtctttcgcc gcaaaaaccg tcaacggacc
     1081 ggcggtctgg ctgaagcggt tgtggatgag gtggggcaga tcgcggatgt gatccgcgtg
     1141 ggtatgagta atgaaaacag cctggatgcg ggcttgctgc gcgaaggaga gcttggatgt
     1201 gagggtcccg gcgtcgactg caaaccagtc gtcgatcagg tagcccgtca gattatgttc
     1261 agcatctgac gcgccgtacg cgccgagcac cttgagccgc atccctgtcc ttcgcccgtc
     1321 ctgtctgtga ctcgcctgga aggagactat tcttgcagcg gaagaactta atgtccagcg
     1381 ctggaggaag gttcacacat tgaccacctc gcggaaagct cgcccaggct cgccggtcga
     1441 tgcggcaatg gccccgtttc atccggccgt tcgcgactgg ttcgcgaacg tcttccgcac
     1501 gccgacgcgg gcgcaggagc tcggctggcc ggccatcgcg cgcggtgact ggaccctgat
     1561 tttcgcgccc accggcagcg gcaagacgct gaccgctttc ctctgggcgc tcgatcgatt
     1621 gatgttcgct gagcggccgg aaaaggatcg ccggtgccgc gttgtgtaca tctcgcctct
     1681 caaagcgctg gcggtcgacg tcgagcgcaa cctccgcgcg ccgctaacgg gaatcgccaa
     1741 cctcgccgcc gaacgcagtg agagtttcgt ggtcccgtcg atcgcgatcc gcaccggcga
     1801 cacgccgcaa aacgaacgcg cgcgtttcct tcgcgatccc gccgatatcc tcatcacgac
     1861 tcccgagtcg atttacctga tgctgacgtc gaacgcccgc gacgtcctgc gtggcgtgga
     1921 gacgatcatc atcgacgagg tccacgccct tgtcccgaac aagcgcggcg cgcatctggc
     1981 gctgtcgctg gagcgattgc agcgggtcgc cgataaggtt ccgcagcgca tcgggctctc
     2041 cgcgacgcag aatccgttgg aagaggtggc gaggtatctg ggggggttcg gaggtgaacg
     2101 cgaagtcacg attatcgata cacgcgaaag aaagcagctc acgctccacg tcgaagttcc
     2161 cgtcgaggag atggcgaagt tgaacgagtc gtcgcgcgac gacatcccga gcggtccggc
     2221 cggaaagggc gtacgtgcct cgatctggcc ggccatccat ccgcgattgc tggagctgat
     2281 ccgagcgcat cgttccacgc tgatcttcgt caactcgcgg cgcctcgccg agcgtctggc
     2341 cggcgccatc aacgagcttg ccggcgaaat gctcgtgcag gcgcatcacg gctcgatcgc
     2401 gcgcgcgcaa cggatcgaga tcgaagacaa cctcaaatcg ggacggctgc ccgcgctcgt
     2461 ggccacctcg tcgctcgagc tgggtatcga catgggcgcc atcgatctcg tcatccagat
     2521 cgaagcgccg ccgtcgatcg ccagcgggat gcagcgcatc ggccgcgcct cgcaccaggt
     2581 gggcaccgtc agcgagggga tcatcttccc gaagtttcgc ggcgatctgc tggcgtgcgc
     2641 cgcggtcacc gaagcgatgc atcgcggcgc gatcgagccg acgcgctatc cgcgcaatcc
     2701 gctcgacgtg ctggcgcagc aggtcgtggc gatgatcgcg accgactcgt accacgccga
     2761 cgaccttttc gccctcgtcc ggcaggccgc gccgttcgcc gagctgtcgc gaacgtcatt
     2821 cgacggcgtg ctcgacatgc tctccggcct ctacccctcg gacgaattcg cggagctgcg
     2881 gccgcgcatc acgtgggacc gcgtcacgaa gatcctcacg ccgcgcccca atgcgaaacg
     2941 cgtagccatc accagcggcg gcacgatccc ggaccgcggt ctcttcggcg tcttcctcgc
     3001 cggtggcgaa aaggtgcaga gcgcccgcgt cggcgagctc gacgaagaga tggtcttcga
     3061 gtcccgcgtc ggcgagacct tccttctcgg cgcttcgacc tggcgcatcg aggagatcac
     3121 gcacgatcgc gtgctcgtca cgcccgcgcc cggcgagcca ggcaagatgc cgttctggaa
     3181 agccgacgcc gccgagcgca cggttccctt cggcgaagcg atcggcgcgc tcgtccgcga
     3241 gatcggcctg atgcagcccg ccgccgcaat caagcgcctg acgaccaagc atgacctcgg
     3301 cgagcttgcc gcgacgaact tcatccaata tctcgacgat cagcaggccg ccaccggcgc
     3361 ggtccccgac gatcgcacca tcgtcatcga gcgcgtcctc gacgacctcg gcgactggcg
     3421 cgtctgcgtc ctcacgccgt tcggcggtcg aatccacgcg ccgtgggcga tggccgtgac
     3481 cgcgaaggtg cgcaacgagc tgcagatcga cgtcgagaca atgtggagcg acgacggttt
     3541 catcgtccgt ttccccgata ccgacgctcc gccgcgcgcg gaactgctca tccctgacgc
     3601 ggacgagatc gagcagcttg tcgtcaggca gctcggttcc agttcgatgt ttgcggcgaa
     3661 gttccgcgaa gccgccgccc gagcattgct gctaccgcgc tttcgccccg accgccgcca
     3721 accgctctgg cagcagcgca aacgcgcgta cgacctcttg caggttgcct cgcgattcgg
     3781 ctcgttcccg atcatcctcg agacctatcg cgaagtgttg cgcgacgtgt tcgatgtccc
     3841 cgcgcttgtg gcgacgatga aaaagattca gcagcggacg atccgcgtcg tcgttgccga
     3901 caccgagaag ccgtcgccgt tcgccggctc gctcctcttt cgctatgtcg ccaacttcat
     3961 ttacgacggc gacgcgcccc tcgccgagcg gcgtgctcag gcgctggcca tcgatcaggc
     4021 ccagctccgc gagctactcg gcgaaccgga gctgcgcgag ctgctcgatg ccgatgcgct
     4081 caacgcgctc gagatcgagc tgcagcatct cgatgagcgc cacaaagcga agtcgctcga
     4141 tgcccttcac gatctcttcc tccgcctcgg cgatctgagc ttcgaagagg tcgtcgcccg
     4201 atcgctcctc actgttggcc cgtcgtcgac gccggacggc gcaacgatcg aatcgcaagt
     4261 caaagccgca atccgcgagc tggtgaagac tcgccgcatc atcgaagtcg gagtcgcgcg
     4321 cgagaagcgc tttatcgcca tcgaagacgc cagccgctac cgcgacgccc tcggcgcgcc
     4381 gcttccgccc ggactggccg atcgttacct gcagccggtc gccgatccca ctggcgatct
     4441 cgtgctccgc tacgcgcgta cgcatggccc gttcacgccg cacgatgtcg cgcatcgcta
     4501 cggcctcggt gtcgccgtcg tgacgaattc gctggaacga ttcgtcgagc gtggccgcct
     4561 catcgaaggc gagtttcgtc ccggcggcac gcagcgcgag tggtgcgaca acgatgtgct
     4621 cagcgccatc cgccgccgtt cgctggcgaa acttcgcaag gaagtcgagc ccgtcgagac
     4681 gaaggcgtac gtccggctca tcaaccattg gcaattcgtc acgaagaaaa ggcgcggcct
     4741 cgaagggctg ctcgaagtga tcgaagcgct gcaaggcgcc gcgctgccgg cctcgatctt
     4801 cgagtcggag atcctggccg cgcgcatcga cggttacaaa ccgtctgatc tcgacacgct
     4861 cagcgccgcc ggcgagatca tctggaccgg cgtcgagccg ctcggctatc gtgacggccg
     4921 cattgcgctc tacctcaccg atcaccttcc gctgttgcat tcacccgtcg cgacagacgc
     4981 aagcgagttg gagcagcaga tcatcgatca ccttcgcgcg agcggcgcct ccttcttcgc
     5041 cgaggtccag actgcgctcg gcggcttcgc gaacgacgtc gtcgacgcac tctgggccct
     5101 cgtctggcgc ggcgtaatca ccaacgacac gttccacgcc ttgcgcgcgt acacgcgccc
     5161 gaaggcctcg ccgcgcacga ccggttttcg ttcacgccgc gtcgcgccgc cgtcgacgca
     5221 aggccgctgg tcgctgatcc aacctccgcg cggcacggcg accgaacgcg ccagcgcgat
     5281 cgcgcatcag ctactggcgc gctacggaat cgtggtgcgc gaagccgccg cggtcgaaag
     5341 catccccggc ggattcagcg cgatctatcc cgtgctcaaa ggaatggagg agtcgggccg
     5401 cacgcgccgc ggctacttcg tggccggctt cggcgcaaca cagttcggca gcgccggcgc
     5461 gctcgatctt ctgcgctcgt atcgtgacga gcccgacgag ccggaaaccg tgatgctcgc
     5521 cgccaccgat cccgccaatc catacggctc gctgttgaag tggcccgttg ttggcctgat
     5581 gcgcgttccc ggcgcgaacg tcatcctcgt caatggcatg ctcggcgcct acatcggccg
     5641 cggcgaaaaa caactttccg ttttcctccc cgagaacgaa ccaatgcgtg gcgtcgtcgc
     5701 gcgcgagatc gcgaagacac tggcctcgct cgtaacgaac ggcgtccgcc gcgcgatgct
     5761 gatcgccgag gtcaatgacg caccggcagc gaaatcggat ctcgcgccgt atctcgccga
     5821 ggcgggattc gtgccgacgg cgatggggta ccagatgcgg gcacttcgaa tctgagcgat
     5881 gcaaagcgat gcctgaaggc gacagcatct accgcgcggc ccaggcgctg catcgtgcgc
     5941 tggcaggcaa gacggtcaca tcgttcgaga ccgtgcttcc caaactcgcg cgcatcgatg
     6001 aagacgcgcc gctcgcaggc cgcacgattg agcgcgtcat ttccgcgggc aagaatctca
     6061 tcatcgaatt ctccggcgat cttcaccttc acacccacat gaagatgaac gggagctggc
     6121 acatctaccg gcccggcgag cgctggcagc ggccgcatcg cgacatgcgc atcgtgatcg
     6181 ggacggcgga tttcgtagcc gttgcgttca ccgttccggt cgccgagttt cacacgagcc
     6241 gcagcttggc gcgcgacgag aacctccgcc gcatcggccc cgacctcctc ggcgcgacgt
     6301 tcgatcgcgc cgaggcgctg cgccgcatcc gcgcacatcc gaacgaggag atcgcgaacg
     6361 tcctcctcaa ccagcgcgtc atcgccggca tcggcaacat ctggaaatcg gagacgctgt
     6421 tcgacgctgg catcgatccg ttcacgcacg tctccgatct cgacgaaatc caactcgatc
     6481 gaatcatctc ctccgcgctg aaactcctcc gccgcagcgc cgaaggccgc gcggaattcg
     6541 ccgtctacag ccgcggcggc gacccctgcc gcaaatgccg caccccgatc cgctatcgca
     6601 aacaaggcga agacgcgcga ggaacctatt ggtgtcccgt gtgccaaagg agtgcaggcg
     6661 tctcgcctgc ggccggcggg cgtcccgccc gacggtgatt gcggacaaga atgcggaatt
     6721 tagagacgga atcgtgacca agcgttgccg gcttcaccaa aaccacgccg aggcgaggac
     6781 gcctcgtcgc cgcaggcgag acgcctgcac tccttgtatc ctgcaaacgt ggcgcgcgat
     6841 cttcctatcg gcaacggcag cctcatggtc aacttcgatc atgagtacaa catccgcgac
     6901 gtttactacc cgcacgtcgg gcaggacaac cagaccaacg gggacgtttc gtttttcggc
     6961 atctggtgcg atggaaagtt cgcgtggatc ggcgatccgt cgtgggtgaa gcggctcgat
     7021 tacgacgggg acacgcttgt cacgcggatg gaagcgcact ccgattcgct gcagctcgac
     7081 atgaccatca atgatgcgat cgacttcgat cgcgatgtct tcatccgccg tgtcgagctg
     7141 cgcaatttgg cggacaagaa acgcaccgtg cgtctcttca gccatttcga tccccatctc
     7201 agcggcaacg ccatcggcga cacgatcttc ttcgatcccg agttcaacgg attcgtcgcc
     7261 tacaaagcgc agcgctacgt gctgatcaac gcggcgagcg acgaccagtt cgggttcaag
     7321 gccttcgcca tcggtaccaa ggagcgcgag ggttatcagg gaacctggcg agacgccgag
     7381 gatggcgtgt tcgagccaaa taaggcaatt gcgcagggat cggtcgattg caccggaatg
     7441 atcgagatca cgctgctgcc gggcgcggtc tcgacggctt acgtctggtg ggtcttcggg
     7501 cagtcgtaca aggatgtggt ggcgctcgac accttcgtgc gcgagcgcgg cgcggcgtcg
     7561 ctgctcgacc gtacacgcga ctactggaag ttgtgggtca agaacgacgt cgacatcagc
     7621 cggttgcccg aggcggttca aaggctgtat cgacgatcgc tgctgatcat ccgcacgcag
     7681 gtcgatca
//
LOCUS       DATLVW010000319        10252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_423444, whole genome shotgun sequence.
ACCESSION   DATLVW010000319 DATLVW010000000
VERSION     DATLVW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423444"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1812
                     /locus_tag="VNN25_09835"
     CDS             133..1812
                     /locus_tag="VNN25_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH91868.1"
                     /translation="MRRLSHVVVSFALLVVPCVFGQNVGPLPPLTAHIDVKIINVDVT
                     VTDHAGRPVTGLTKDDFEIFEDGKLQTVKNFDIIENAVLRGTTPSAPSSASTPFSDQS
                     RRKFVLLVDNNYIDKIERDRALAEIDAYVDKAFGGEHEWAIAAISQRLEMLHPFTSDR
                     KEIHAAIDRIRKSGTFPEQHAIDRSILSDGIRRIELSASATTEDAGLLSAVDYGTIVR
                     FQAREQTWRALRAIRNTANAVREMTHAYAASEGKKVLILITGGMETNTNFTAYTRGQP
                     DREITEAQHQIGIALDDVVLEANASNFAIHIINAKRRGMIAPQHDVENARSGVDPSSD
                     LSVKISSPIDTTDVDSSSVTLAEGTGGSYSRNPIDRSLAAVETLSSNYYSLGYTPPHE
                     DDRRYHSIKVRVKRSGVNVANRQGYIDLSPEDRLEQFLQARFSAEEKPGSLPIAIQIG
                     GAVPSGDEVSVPVTTSLRMDRLTVIPVDGQYVGRLHVYVSVFDSEGKNVGFNHQLQEV
                     TIPSAQYGQIGDSSFRYKLNVHLKKGAFTIVMTVRDDLSNEMGSASKGVSI"
     gene            1863..3434
                     /locus_tag="VNN25_09840"
     CDS             1863..3434
                     /locus_tag="VNN25_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH91869.1"
                     /translation="MASTAAPQTQSKPIPLASSIDVRVINVDVTVTDGSGKPVTDLAK
                     GDFEVLEDGQPQPVTNFSLNQRKVAVAGSSAEPSTAPRRKVIVVIDNNYIDARQRSLT
                     LDTLDRFVEERFDDNSEWSIATIGQSLDIIQPLTNSRAKIREAVAKARKSGTVSLRTD
                     ELDREILSDPFRRTAKGGYDYEETVRFQARERTSRNELALANTAKGLTEAARSYSGVD
                     GKKIFVLLTGGMEMNTSFAAYDNGHDHEMSDRKTGIAKLIEQIVLEANTGNLAIYVIN
                     VRPSDMAAPQHDVENQAWGGRVGASSTGESDISDVDSAAFTLAASTGGLNFTANVVRK
                     SLDAVDAMSANYYSLGYGPQHVEDGKYHTITVRLKKPGLKALHRRGYMDVSPDVQLEQ
                     YLRLRISILQPSNTVAVNVDTHSSVGPDGKPVVQLTGGMPWQQLTLLRTGEQYKGRVH
                     VYLAIFDKNGANVGFHHQIQDVALTPEQYALAMKGAFRYRMSVRLEKGDYTVAMTMRD
                     DLSRDVGTGVQRLRL"
     gene            complement(3511..3780)
                     /locus_tag="VNN25_09845"
     CDS             complement(3511..3780)
                     /locus_tag="VNN25_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91870.1"
                     /translation="MRISFLRCGSLFLDRDLTLVDPDLSPVRRAPSRDDHTNDEAVSI
                     RLLNALQLSGGTLHVQSSAFSSHAFCMEEGDEADVGALAGTEKLA"
     gene            complement(5365..7749)
                     /locus_tag="VNN25_09850"
     CDS             complement(5365..7749)
                     /locus_tag="VNN25_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH91871.1"
                     /translation="MRRHVLVILVLVLIAIALAAALITLQPADRARVTRNGNSITVQT
                     GRIGLRTFGGKPCFVPIEGNQLYFKQTVAAETRTGDIVSVPVSFLYEALSTLPPDWPE
                     GDWCTSLNVFVQQSLTRALHELPADALIEHNREAADRISKSLEDDLQRARLSAKSVAA
                     RIVLPPGWQQTLPIPEIARAVQPQSPVIFIGLDGGDWQLLDDYVANGTMPNLARLVRE
                     GASGELETEQPPLSPIVWSTMMTGVSPIEHQVLDFTRFNPVTGVKEPITSTERKAPAI
                     WNMETMAGRRVAVFGLWATYPAEPVRGLMVSDRLFPFFFTAESSPPPGAVYPPSREAW
                     ARAGVTAAEAVVDLPAMRRYLPDLTQADFDSLLHDPNPFSKPPSALRRMLVETEIYRH
                     LSVDLFAAGNIPDLTIIYFEGTDTVGHIFAPFAPPKQPQVSQADYDRFHAIPAKYFGF
                     LDQFLGDVLLVADQSHATIVIASDHGFQWKEGRPQQLASFAIATAAKWHRNQGIYLLR
                     GPGIAPSKGHTGRGGIRQICATLLAATGLPPAEHVAGPPLPPLANANASAINYASHFQ
                     PFAPPAATIANSAGADEAVAKLKSLGYIGTGESTAPRPEGSGASTRTGGSYNNEALIL
                     KHEGRIPEAIAAFEKAMQVDPKLSSAAWNLSDLLFDKNRDLRRSDELLVRAIANGHPE
                     APGFAIGRAIGYQRAGHLDWSRKLLEDAVAAKPDDTALRMFRGRYRVAQNECAGALDD
                     FVTVERLKPDDPVAWASAGLAQICLGNRAEAMANIRHSLELDPNQPKLRQFLQQ"
     gene            complement(7843..>10252)
                     /locus_tag="VNN25_09855"
     CDS             complement(7843..>10252)
                     /locus_tag="VNN25_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH91872.1"
                     /translation="VKQLNFDFVQEVEVKTGGLPAEYGRTTGGMINAITKSGGNEFSG
                     DVFGFDQPSGLRSSNATLTQRPHTAGSFTDDTLAPTSQLDYGLDTGGYFVKDRLWFFG
                     AFDRNDQTRSNIRINSPLAIQATPELPGGYNLPVGATIDTKVKSQLFAGKLTLRATES
                     QNLTLSLFGDPTKTSGALFGISGPPTTTQGTRKTGGSDYIGRYSGIFTTKFLVNAEAG
                     HHHEDATISGAGTGIAQLIDQSVSPNVTSGGYGFFDNNTYDRDLAKLSATAFLSNHEF
                     KVGGDYEKMQANVENFQGGAGQRIYKFKTSGGTVYYRHRYYVDDLASGFNQADPTTWK
                     IALPQVAKPETKNNSLYAQDSWRVLPNFTVNAGLRWESQELLGRGGVVATKINDNWAP
                     RLGVIWDVANNGRSKLYANAGRFYENIPMDINIRSFGGELVCFCYNFSADPANLRPDS
                     TAPKRSSLLGGATPVDPNLKGQYIDEFLGGYEYEIRPNLALGIKGTYRKLGRVIEDML
                     QVPITGDYAITNPGTGLGRESGFYNYEDSVVVPKAKRTYKGVEISANKRFSDNYQFFA
                     SYVWSRLEGNYDGTFQASTGQLDPNINSAYDYADFLVNNHGLLSNDRTHQLKFYGSYQ
                     FSGTSMLNGLDVGFSAHYASGTPLTAVGYSAGYANWEYYLTTRGAAGRGPADYEADLH
                     LDYPIKMGTGRMSIIADVFNILDRQAKTALDQRYNRVQDGVCGGFVAICDATNVLDAN
                     GKKTNLNPGGAALGGGGVAVKPGTLTPIGSINLANAPNPDFLKAGTSFTSPRTFRLGA
                     RYTF"
BASE COUNT         2081 a         2969 c         3200 g         2002 t
ORIGIN      
        1 ctctgatctc tgcttctccg actcccgacc cccgactccc gactccccac tgttacgcct
       61 gaggaacagc tcggcgatcc gcacccataa atccgtcccg gacgtacaat cggcgcttca
      121 gggggaggat ctatgcgccg gttgtcacac gtggttgtgt cgtttgcgtt gctcgttgtc
      181 ccgtgcgtct tcggtcagaa cgtcgggcct ctgccgccgc tgacggcgca catcgatgtg
      241 aagatcatca acgtcgacgt gacggtcacg gaccacgcgg gacgtccggt cacgggcctc
      301 acgaaggatg acttcgagat cttcgaagac ggaaaactgc agacggtcaa gaacttcgac
      361 atcatcgaga acgccgttct gagaggaacg acgccatccg ctccttcttc cgcctccaca
      421 ccattctcgg atcagtcgcg gcgcaagttc gttctgctcg tcgacaacaa ttacatcgac
      481 aagatcgagc gcgatcgggc tctcgcggag atcgatgcct atgtcgacaa ggccttcggc
      541 ggcgagcatg aatgggccat tgccgcgatc agccagcggc tggaaatgct gcacccgttc
      601 acgtcggaca ggaaggagat ccatgccgcg atcgacagga tccgcaagag cggcacgttc
      661 ccggagcagc atgcaatcga ccgctcgatt ctgtccgatg gcatacgccg catcgagcta
      721 tccgcatccg ctacgaccga ggatgccggc ctcctttcgg cagttgatta cggcacgatc
      781 gtccgtttcc aggcgagaga gcaaacctgg cgcgcgcttc gtgcgatccg taacacagcc
      841 aacgcggtcc gtgagatgac gcacgcctac gccgcctcgg aggggaagaa agtcctgatt
      901 ctcatcaccg gcgggatgga aacgaacacg aacttcacgg cgtacacgcg gggacagccg
      961 gaccgtgaga tcactgaggc acagcaccag attgggatcg cactcgatga tgtcgtcctc
     1021 gaagcgaacg cctcgaattt tgccattcac atcatcaacg ccaagcggag agggatgatc
     1081 gctccgcagc acgacgtgga aaacgcccga tcgggcgtcg atccatcatc cgacctgtcg
     1141 gtcaaaatct cctctcccat cgacacgacc gacgtcgatt cgtcatcagt cacgctcgcc
     1201 gagggcacgg gcggttcgta ctcgaggaat ccgatcgatc gttcgctggc cgccgtcgag
     1261 acgctgagct cgaactatta ctccctcggc tacacgcctc ctcacgagga cgaccgccgc
     1321 tatcactcga tcaaagttcg tgtgaagcga agcggcgtga acgtcgccaa ccgccaggga
     1381 tacatcgatc tttctcctga ggaccggctc gaacagttcc tccaggcccg cttctccgcc
     1441 gaggagaaac ctggatcgct gccgatcgca attcagatcg gcggagcggt tccgtcgggt
     1501 gatgaggtca gcgttccggt gaccacctct cttcggatgg accggttgac ggtcattcct
     1561 gtcgatggcc agtacgtcgg ccgccttcat gtctatgtct ccgtctttga ttccgagggg
     1621 aagaatgtcg gcttcaatca tcagttgcag gaggtgacca ttccttcggc gcagtatgga
     1681 cagatcggcg attcgtcgtt ccgctacaag ttgaacgtcc acctgaagaa gggcgcgttc
     1741 accatcgtga tgacggtaag ggacgatcta tcgaacgaga tggggagcgc atcgaaaggt
     1801 gtgagcatct aggtccggcg atgatccccc gaggagtgtt tctttgtagt gcaatggtcc
     1861 tcatggcttc gactgctgcg cctcagacgc agtcgaagcc aattccgctc gccagcagca
     1921 tcgacgtaag ggtcatcaat gtcgatgtca cggtgaccga tggcagtgga aagccggtca
     1981 cggatctggc gaagggagat tttgaagtcc tggaggatgg gcagccgcag ccggtcacga
     2041 atttctccct gaatcagcgg aaagtggcgg ttgcgggctc ctccgcggag ccatcgacgg
     2101 cacctcggcg aaaagtgatt gtcgtcatcg acaacaacta catcgacgca cgccagcgct
     2161 ctctgactct cgacacgctc gatcgatttg tcgaagagcg cttcgacgac aactcggaat
     2221 ggtcgatcgc gacgatcggc cagtcgctcg acatcattca gccgctcacc aacagccgcg
     2281 cgaaaatccg cgaagcggtg gcgaaagcac gcaagagcgg aacggtctcg ctccgcaccg
     2341 atgagctcga tcgtgaaatc ctgtccgacc cgtttcgccg cacggcgaaa ggtggctacg
     2401 actacgaaga gacggttcgc tttcaagcca gggaacggac gtcgcgcaat gagctggcgc
     2461 tcgccaacac ggcaaagggg ttgaccgagg ccgcgcgatc gtactcaggt gtcgacggaa
     2521 aaaagatctt cgtacttctc accggcggca tggagatgaa tacgagcttt gccgcgtacg
     2581 acaacggcca cgatcacgag atgagcgacc gcaagacggg cattgcaaag ctcatcgagc
     2641 agatcgtgct cgaggcgaac accggcaatc tggcgattta cgtgatcaat gtccgtccca
     2701 gcgacatggc cgcgccgcag catgacgtgg agaaccaggc ctggggcggg cgagtcggag
     2761 ccagctccac gggagaatcg gacatcagtg acgttgattc ggcggcgttc acgctggctg
     2821 cctcgacggg aggtctcaat ttcacggcga acgtcgtgcg caagtcgctc gatgccgtcg
     2881 atgcgatgtc ggcgaactac tactcactcg gatatggacc gcagcacgtt gaagacggga
     2941 agtaccacac catcacggtg cgcctgaaga agccgggcct caaagccctt caccgccgcg
     3001 gatacatgga tgtttctccc gatgtgcagc tcgagcagta cttgcggctt cgcatctcga
     3061 tcctgcagcc gtccaacacg gttgcggtga acgtcgacac ccactcatcg gtgggacctg
     3121 acggaaaacc agtcgtccaa ctcactggcg gcatgccctg gcaacaactc acactgctcc
     3181 gaaccggcga gcagtacaaa gggcgggttc atgtctacct cgccatcttc gacaagaacg
     3241 gagcaaatgt cgggtttcac caccagattc aggatgtcgc gctgacgccg gagcagtacg
     3301 cactggcaat gaaaggcgcg ttccggtatc gcatgtcggt ccgcctggag aagggtgact
     3361 acacggttgc gatgacgatg cgcgacgacc tgtcgcgcga tgtcggaacc ggagtgcaga
     3421 ggctgcggct gtagccggca tttctcacac ctttgaagaa attcggcgct cccttgcatc
     3481 gggcggatga gggatggaaa acgctcgatt tcatgccaac ttttccgtgc cagccaatgc
     3541 ccccacatcc gcttcgtcgc cttcttccat acaaaaagcg tgggaggaga aggcgctcga
     3601 ttgaacgtgc agcgtgcctc cacttagttg caacgcattg agaaggcgga tacttactgc
     3661 ctcgtcattg gtgtgatcgt cgcgggaagg agcacgcctc accggcgaca aatcgggatc
     3721 taccagggtt agatcgcgat ctaaaaaaag agatccgcat ctcaaaaaag agatccgcat
     3781 ctcttaaaaa gagatccgca tctcgaaaaa gagatccgca tctccaagaa agagatccgc
     3841 atctcctcgg aagagatccg catctcctcg gaagagatcc gcatctcccc ggaagagatc
     3901 cgcatctcct tggaagagat ccgcatctta aaaaagagat ccgcatctcc caagaagaga
     3961 tccgtatctc ccaagacgag atccgtatct cccaaaaaga gatcgcgatc tgccaaagac
     4021 agatcgtgac atgccggaca gagatggaga cctcgccgat cctgatcgca tccacccctc
     4081 cgagatgagc cggcacattg gcagtcaatg gaaggcagcg cattccaatc gagcgccttc
     4141 tcccccacgc ttttcagtgg gggagaaggt ggccgaaggc cggatgaggg gggctttggc
     4201 tatcgcggca acgttgcaca aacagtacgc acggaggcct tttcaatcgc ctctctcgct
     4261 cgcaaagaac gtgttcgtcc tatcttgcaa cgcccccctc atccggcttc gccaccttct
     4321 ccccccgcaa aaagcgcggg gggagaaggc gctcgattgg aaagagccga ggatagaggt
     4381 gtgagaaatg cgagttagtt agcgtgatcc ctttttcggg atctcctccc gggaaatcgc
     4441 gacctcgcct gaacagatcg cgacttcgtt ggaaggggat tgcgacctcg gggaagggaa
     4501 ccgcggcgac tactgctgga ggaactgacg gagtttcggc cgatcctgat cgcatccacc
     4561 cctccgagat gagccggcac attcgcagtc gattgaaggc agcgcattcc aatcgagcgc
     4621 cttctccctg cttttcagtg ggggagaagg tggccgaagg ccggatgagg gggcgtttgg
     4681 ctatcgcggc aacgttgcac aaactcgcag ggaggccttt ccaatcgcct ctctcgctcg
     4741 caaagaacgt gttcgtccta tcttgcaacg cccccctcat ccggcttcgc caccttctcc
     4801 ccccgcaaaa agcgcggggg gagaaggcgc tcgattggaa ggagcggagg ataggtgtgt
     4861 gacaaatgcg agttagttag cttgatccct tttgcgggat ctcctcccgg gaaatcgcga
     4921 cctcgcctga acagatcgcg acttcgttgg aaggggattg cgacctcggg gaagggaacc
     4981 gcggcgacta ctgctggagg aactgacgga gtttcggccg gatcgcatcc atcctccggg
     5041 atgagccggc acattggcag tcaattgaag gcagcgcatt ccaatcgagc gccttctccc
     5101 ccacgctttt cagtggggga gaaggtggcc gaaggccgga tgagggggcg tttggctatc
     5161 gcggcaacgt tgcacaaaca gcacgcaggg aggcctttca atcgcctctc gctcgtaaag
     5221 aacgtgttcg tcctatcttg caacgccccc ctcatccggc ttcgccacct tctccccccg
     5281 caaaaagcgc ggggggagaa ggcgctcgat tggaaggagt cgtgaacaag tgacaaatgc
     5341 gggcaagcga aacgaccgcg gcgactactg ctggaggaac tggcggagtt tcggctgatt
     5401 cggatcgagc tccagggagt ggcggatgtt ggccatcgct tcggcgcggt tgccgaggca
     5461 gatctgggcg agaccggcgg acgcccatgc cacagggtca tcgggcttca accgttccac
     5521 ggtgacgaaa tcgtccagcg cgccagcgca ctcgttttgc gctacgcggt agcggccccg
     5581 gaacatgcgg agcgcggtgt cgtcaggctt agcggcgact gcgtcttcga gaagcttccg
     5641 gctccaatcg aggtgaccgg ctcgctgata tccgatggcg cggccgatgg cgaagccggg
     5701 cgcctcggga tgtccgttcg cgatcgctcg aacgagaagc tcatcggagc gccgcaaatc
     5761 ccgattcttg tcgaaaagca gatcgctcag attccacgcc gcggacgaca gcttcggatc
     5821 gacttgcatc gccttttcga aggcagcgat ggcttccggg atgcgtcctt cgtgcttcag
     5881 aatcagtgct tcgttgttgt aggagccgcc tgttctggtc gaggcgccgg agccttccgg
     5941 acgcggagca gtcgattctc ccgttccgat gtagccgagg gatttgagtt tcgcgaccgc
     6001 ttcgtctgcg ccggcactgt ttgcgatcgt cgcggccggt ggtgcgaatg gctggaagtg
     6061 gcttgcgtaa ttgatggccg aggcgttcgc gttcgcgagc ggaggaagag gcggaccggc
     6121 cacatgttcg gcgggaggca atccggtcgc ggcgagaagc gtcgcgcaga tctggcggat
     6181 tcccccgcgg ccggtgtggc ctttcgacgg agcgatcccc gggccgcgca gaagatagat
     6241 cccctgattg cgatgccatt tggcggcagt ggcaatggcg aagctggcca gttgctgcgg
     6301 gcgcccttcc ttccattgaa agccgtgatc cgatgcgatg acgatcgtgg cgtgcgattg
     6361 atcggcgaca agtaggacgt ccccgaggaa ctgatcgaga aatccgaagt acttcgctgg
     6421 aatcgcgtgg aaacggtcgt agtcagcctg actgacctgc ggctgttttg gcggagcaaa
     6481 cggcgcgaag atgtggccga ccgtgtccgt tccctcgaaa tagatgatcg tcagatcggg
     6541 gatgttgccg gcagcaaaaa gatcgacgga gaggtggcga tagatctcgg tttcgacgag
     6601 catccgccgc aaagcgctcg gcggcttcga gaatggattg ggatcgtgca gcaacgaatc
     6661 gaaatcggcc tgtgtgagat ccggcaggta acgacgcatc gccggcagat cgacaaccgc
     6721 ctcggcggca gtaacaccag cccgcgccca cgcttcgcgc gatggcggat agaccgctcc
     6781 gggcggcggc gacgactcgg cggtgaagaa gaaggggaac aggcgatccg agaccatcag
     6841 tccgcgcacc ggctcggcgg ggtaggttgc ccacagtccg aagaccgcca cgcgccggcc
     6901 agccatcgtc tccatgttcc agatcgccgg cgcttttcgt tccgtgctgg tgatcggctc
     6961 tttgacgccg gtgaccggat tgaaacgcgt aaagtcgagc acctgatgct cgatcggact
     7021 gacgccggtc atcatcgtgc tccagacgat gggcgagagg ggaggctgct ccgtctccag
     7081 ctcgccactg gcgccctcac gcacgagacg agcaagattc ggcatcgtac cgttggcgac
     7141 gtagtcgtcg aggagttgcc agtcgccgcc atcgagtccg atgaagatga cgggagattg
     7201 gggctgcacg gcgcgggcaa tttcggggat cgggagcgtt tgttgccagc ccggcggaag
     7261 cacgattcgc gcggccacgc tcttggcgga gaggcgcgcg cgttggagat cgtcttccag
     7321 tgacttggag atcctgtcag cggcttcgcg attgtgctcg atgagcgcgt ctgccggcag
     7381 ctcatgcaaa gctctggtga ggctctgttg cacgaagaca ttgagtgagg tgcaccagtc
     7441 gccctcgggc caatccggag gaagcgtgga cagcgcctcg tacagaaagc tgaccggaac
     7501 gctgacgatg tcgcctgtcc tggtctcggc cgcgacggtc tgcttgaaat agagctgatt
     7561 cccttcgatc ggaacgaaac acggctttcc gccgaacgtg cgcaggccga tgcgtcccgt
     7621 ctgaaccgtg atgctgttgc cgttccgcgt gacgcgcgcg cgatcggctg gctgcagtgt
     7681 gatcaacgcg gctgcgagtg cgatggcaat caggacgaga acgagaatga cgaggacgtg
     7741 gcgacgcatg gaagtgggcg ccttcggagg cggcgcgtat cgtacgcgaa aaaaaaggcc
     7801 ccgcgagcgc ggggcctttt cactactggt tgaacgaacg aactagaacg tgtagcgagc
     7861 tcccagacgg aaggtgcgcg gggacgtgaa tgaggtaccc gctttcagga agtcggggtt
     7921 cggagcattg gccagattga tcgatccgat cggcgtaagc gttcccggct taacggcaac
     7981 tccgccacca ccgagcgccg cgccgcccgg attgaggttg gtcttcttgc cattggcgtc
     8041 gaggacattg gtggcgtcgc agatagccac aaagcctccg cagacaccat cctgtactcg
     8101 gttgtatcgc tgatccaacg cggtcttcgc ctgacgatcg aggatattga agacgtccgc
     8161 gatgatgctc atacgtcccg tgcccatctt gatcggatag tcgagatgaa ggtccgcctc
     8221 ataatcggca gggccgcgtc ccgctgcgcc gcgtgtcgtc aggtagtact cccaattcgc
     8281 gtagccggcc gagtaaccga ccgccgtcaa cggcgttccg gaagcgtaat gggctgagaa
     8341 accgacgtcc agaccgttga gcatgctcgt gccggagaac tggtaggagc cgtagaactt
     8401 gagctgatgc gtgcgatcgt tgctgagcag tccgtggttg ttgaccagga agtcggcgta
     8461 gtcgtacgcg gagttgatgt tcggatcgag ctgaccggtc gacgcctgga aggtgccgtc
     8521 gtagttgccc tcgagccgtg accagacgta actggcgaag aactgatagt tgtcgctaaa
     8581 gcgtttgttg gcagagattt cgacgccctt gtacgtgcgc ttggccttcg gtacgaccac
     8641 gctgtcttcg tagttataga aacctgattc acgtcccagg ccggtgcccg gattggtgat
     8701 ggcgtagtca ccggtgatgg gaacctggag catgtcttcg atgacgcgtc cgagcttgcg
     8761 gtacgtgccc ttgatgccga gggccagatt cggacggatt tcgtattcgt agccgccgag
     8821 gaactcgtcg atgtactgac ccttcaggtt gggatcgacc ggcgtggcgc caccgagcag
     8881 ggagctgcgt ttcggcgcgg tcgaatcggg gcggagattt gcgggatcgg cgctgaagtt
     8941 gtagcagaag caaacgagct caccgccgaa ggaacggatg ttgatgtcca tcgggatgtt
     9001 ctcgtagaag cggccggcgt tcgcgtacag cttgctgcga ccgttgttgg ccacatccca
     9061 gatcacgccg agtctcggcg cccagttgtc attgatcttc gtggccacga cgccgccgcg
     9121 gccgagaagt tcctgtgatt cccagcgaag accggcgttg accgtgaagt tcggcagcac
     9181 gcgccagcta tcctgcgcgt acagtgagtt gttcttggtc tccggctttg caacctgcgg
     9241 aagagcgatc ttccaagtcg tcggatcggc ctgattgaat cccgatgcca gatcgtcgac
     9301 gtagtagcgg tgacggtaat agacggtgcc gccggaggtc ttgaacttat agatgcgctg
     9361 tccggcgccg ccctggaagt tctcaacgtt ggcctgcatc ttttcgtagt cgccgccgac
     9421 tttgaattcg tgattggaga gaaacgccgt agcgctgagt ttggcaaggt cgcggtcgta
     9481 cgtgttgttg tcgaagaaac cgtatccgcc cgaggtgacg ttggggctga cactctggtc
     9541 gatcaactgg gcgatgccgg tgcctgcacc ggagatcgtg gcatcttcgt gatggtgtcc
     9601 ggcttccgca ttcaccagga acttggtggt gaagattccg ctgtagcggc cgatgtagtc
     9661 gctaccaccg gtcttgcgtg tgccctgggt tgtggtgggt ggtccgctga taccgaacaa
     9721 cgcgccgctg gtcttggtcg ggtcgccgaa caacgacagc gtcaggttct ggctctcggt
     9781 ggcgcgcaac gtcagtttgc cggcaaacaa ctggctcttg acctttgtgt cgatcgtcgc
     9841 gccgacggga aggttgtagc cgcccggaag ctccggtgtc gcctggatgg cgagaggcga
     9901 attgatgcga atgttcgaac gagtctggtc gttacggtcg aacgcgccga agaaccaaag
     9961 gcgatccttc acgaagtagc cgccggtgtc gaggccgtag tcgagctgcg atgtcggcgc
    10021 caacgtgtcg tcagtaaagg aaccggcggt gtgcgggcgc tgggtcagcg tggcgttgct
    10081 cgagcgcaga ccgctcggct gatcgaaacc aaaaacgtcg ccgctgaact cgttgccgcc
    10141 ggacttggtg atggcgttga tcatgccgcc ggtcgtccgt ccgtactcgg ctggcagtcc
    10201 gccggtcttg acttccactt cctgaacgaa gtcgaagtta agctgcttga cg
//
LOCUS       DATLVW010000320         4704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_12662, whole genome shotgun sequence.
ACCESSION   DATLVW010000320 DATLVW010000000
VERSION     DATLVW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12662"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..617
                     /locus_tag="VNN25_09860"
     CDS             99..617
                     /locus_tag="VNN25_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH91873.1"
                     /translation="MTLRATLLIATLAVALNTFAEDPLANPAIDMEGHLRIAAEAAKH
                     RQSRRLTEDEFLGMMREEGTIVLDARSRDKYDELHIAGAINLPFPDITVARLNQLLPD
                     KNTRVLIYCNNNFVNAEKAFPSKIARSSLNVSTYITLYDYGYRNVYELGPLLDARTTH
                     IPFEGTTRSKLK"
     gene            1295..3442
                     /locus_tag="VNN25_09865"
     CDS             1295..3442
                     /locus_tag="VNN25_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91874.1"
                     /translation="MNAERRGLVSGALLSLVILLIAAACAHQAVKPSWTYATFKEVDF
                     DPFPGMDFQGRDVPLSSDDLKGRIVWNLWSGDNGGFWDYLATHAFGTADLLRVVTSPR
                     NQRFETYGLFNQPGYKRPAQPDEYGLLIDVPRDARYDFDSKIDLNTYGRSSGIMGLRL
                     FPNPNFDAKAKANWDANRYQNDPKYYTDKNLVRPYVVGMACSFCHIGADPTNPPPDPD
                     EPEYANLNDYVGQHYFKVSEVFAHDLPKNNFVWQLLNSNPPGTLDTAFIATDYLNNPG
                     TMNGIFNLAGRMNVAVPEHVTGGALALKGVTDPQLTPRVLKEGADSVGLPAALSRVYL
                     NIGEYWEEWTRHFNPLIGITKQSPIRVADAQRLSPHWNWSESHAPALAQYLIDVAKPL
                     KLANTPGHEKYLKADEKTLNRGKKVFAQNCARCHSSKQPPAGVNPQTPEGRAWFEQEV
                     MKPDFLDNNFLSAEIRVPVTEVKTNASRSVASNAMRDQIWDNFSSETYKTLPKTGAIQ
                     VWDPFTGKTKPWEVPGGGRGYYRPPSLVSVWCCAPFFHNNALGKHVAKVDVDSRMEAF
                     NDAIEKLLWAEKRDGAGSIWRTTVPSSLQIPVSYAPLLKNLADPDGFIHIGPIPQGTP
                     INLIANTNLQLGGLRKTNEQVTLLIRALKALKQVNDEGLKDDAATQRLLTLVPDFYAL
                     NSCPDFIEDEGHYFGTVLTDADKRALIEFMKTF"
     gene            3453..>4704
                     /locus_tag="VNN25_09870"
     CDS             3453..>4704
                     /locus_tag="VNN25_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXH91875.1"
                     /translation="MPNDPNNPDFDYIVIGSGAGGGPVACNLAKAGYKVGLIEAGIEA
                     QSQAYPVPVFHTYASEDPEMSWEFFVRHYSDEATSKRDSKFVDAEDGVFYPRAGTLGG
                     CTSHHAMITVYGHGSDWDYIVDLTGDASWSSDNMRSYFERIERCTYAGPAPAAGQPNP
                     SRHGYEGWLNTTGPELSLVFGDDALLKTILTAILGAWKEGISGVLPDLIPQDPNDWRT
                     PKFEGICFAPLATLKGRRLGTRELIDTTRAIYPQNLVIRMSNLATKILFDANKKAIGV
                     ECWEGQHLYKADPNVIADPQAGDDYEVKSYYCKREVILAGGAFNSPQLLMLSGIGPKD
                     HLAKFGIDCILDRPGVGMNLQDRYEIDVCTEMKNDWKILANATFAPPLPNAPGDPCYI
                     DWQNGKGVYATNGAVLGVIKKSSPE"
BASE COUNT         1003 a         1553 c         1346 g          802 t
ORIGIN      
        1 gccggcggaa acgatgagag aaggaaagga agtctcttcg agggcgggcg aggaaccttt
       61 ccagcgctcg aacgtccaat agcaaggagg ctttcctcat gactctgcgc gccacgctct
      121 tgattgccac tctcgccgtc gctctcaaca cgttcgctga agacccgctt gcgaatccag
      181 ccatcgacat ggagggacat ctgcgcatcg ccgccgaggc ggcgaagcat cggcagtcgc
      241 ggcggctgac cgaggatgag ttcctcggca tgatgcggga agaaggaacg atcgtgctcg
      301 atgccaggag ccgcgacaag tacgacgagc tgcacatcgc cggcgccatc aacctcccct
      361 ttcccgacat cacggtggcc aggctcaacc agctccttcc cgacaagaac acccgcgtgc
      421 tgatctactg caacaacaac ttcgtaaacg cggagaaggc gttcccgtca aagatcgcgc
      481 gctcgtcgct caacgtctcg acgtacatca cgttgtacga ctacggctat cgcaacgtgt
      541 acgaacttgg gccgctgctc gacgcacgaa cgacacacat tccgttcgag ggaacgacac
      601 gatcgaagct caagtagtgc aggtgtccac acctgctacc gacgggcgtc cctgcccgtc
      661 ggtgattccc ggaaatggaa ccacaactcg tggccctttc cgttggctgt ctggcgccaa
      721 accaccgccg ggcgaggacg cccgtcggtt gcaggcaaga atgcctgcac tacgtttcgc
      781 acccgaacag atcgctacgg agtcggagac cggcggcaac aaaccgccag gccaagtagt
      841 gcaggtgtcc acacctgcta ccgacgggcg tccctgcccg tcggtgattc ccggaaatgg
      901 aaccacaact cgtggccctc tccgttggct gttcggcgca aaaccaccgc cgggcgagga
      961 cgcccgtcgg ttgcaggcaa gaatgcctgc actacgattc gtacccggac agatcgctac
     1021 ggaatcggag accggcggca acgaaccgcc gggcaaagta gtgcaggtgt ccacacctgc
     1081 aaccgacggg cgtccctgcc cgtcggtgat tcccggaaat ggaaccacaa ctcgtggccc
     1141 tttccgttgg ctgttcggcg ccaaaccacc gccgggcgag gacgcccgtc ggttgcaggc
     1201 aagaatgcct gcactacgtt atcgttgcag aatcgaaggt agggaaatcg ctccgcaggg
     1261 cactcctcta gtagcctcta ttggaggaga tccgatgaac gctgaacgcc gaggactcgt
     1321 ttctggtgcc cttctctccc ttgtcatcct cctgatcgct gcggcttgcg cgcatcaggc
     1381 ggtgaaaccg agttggactt atgccacgtt caaggaagtc gatttcgatc ccttcccggg
     1441 gatggacttt cagggacgtg atgtcccgct gagcagcgac gacctcaagg gccgcatcgt
     1501 ctggaatctc tggtccggcg acaacggcgg cttctgggat tacctcgcca cccatgcctt
     1561 cgggactgct gacttgctga gggtcgttac ctcgccgcgc aaccagcgct tcgagacgta
     1621 cggcctcttc aaccagcccg gttacaagcg gcccgcgcaa cctgacgagt acggtcttct
     1681 catcgacgtt ccccgcgacg ccaggtacga cttcgactcg aagatcgacc tcaacaccta
     1741 cggccgctcc agcggaatca tggggctgcg ccttttcccg aatccgaact tcgatgcgaa
     1801 agcgaaggcg aactgggacg cgaatcgcta ccaaaacgat ccgaagtact acacagacaa
     1861 gaatctcgtc cggccgtacg tcgtcggcat ggcctgctcc ttctgccaca tcggcgccga
     1921 ccccaccaac ccgcctcccg atccggatga gccggagtac gcgaacctca acgactacgt
     1981 cgggcagcac tacttcaagg tcagcgaggt gtttgctcac gacctgccga agaacaactt
     2041 cgtctggcag ctactcaaca gcaaccctcc gggcacgctc gacaccgcct tcatcgccac
     2101 cgactacctc aacaatcccg ggaccatgaa cggcatcttc aatctcgccg gccgcatgaa
     2161 cgtcgcggtg ccggagcacg tgacgggcgg cgcgctcgcg ctcaagggcg ttaccgatcc
     2221 gcagctcaca ccgcgcgtgt tgaaggaagg cgccgattct gtcggcctgc cggcagcgct
     2281 cagccgcgtc tatctcaaca tcggagagta ttgggaggag tggacgcggc acttcaatcc
     2341 gctgatcggg atcacgaagc agtcgccgat ccgcgtggcc gacgcgcagc gactctcgcc
     2401 gcactggaac tggagcgaat cgcacgcacc ggcgctggcc caatacctca tcgacgtcgc
     2461 aaagccgctg aagctggcga atacgccggg acacgagaag tacctcaaag ccgacgagaa
     2521 gactctcaac cgcggcaaga aggtgttcgc gcagaactgt gcgcgctgcc actccagcaa
     2581 acagcctccg gccggcgtca atccgcagac tcccgagggg cgtgcctggt tcgagcagga
     2641 agtgatgaag cccgacttcc tcgacaacaa cttcctcagc gctgagatcc gggttccggt
     2701 gaccgaggtg aagaccaacg ccagccgctc cgtggcgtcg aatgcgatgc gcgatcagat
     2761 ctgggacaac ttctccagcg aaacctacaa gacgctgccg aagaccggcg cgatccaggt
     2821 ctgggatccc ttcaccggaa aaacgaaacc gtgggaagtg ccgggtggcg gccgcggcta
     2881 ctaccgcccg ccgtcgctgg tctcggtctg gtgctgtgcc cccttcttcc acaacaacgc
     2941 cctcggcaag cacgtcgcga aggtcgacgt cgattcgcgc atggaagcgt tcaacgacgc
     3001 catcgagaag cttctgtggg ccgaaaaacg cgacggtgcc ggctcgatct ggcggaccac
     3061 ggttccaagc tccctgcaga tcccggtgtc gtacgcgcca ttgctcaaga acctcgccga
     3121 ccccgatggc ttcattcaca tcgggccgat tccacaaggg acaccgatca atctcatcgc
     3181 caacacgaac ctgcaactcg gcggcctcag gaaaacgaac gaacaggtta cgttgctgat
     3241 ccgagcgctc aaggcgctca aacaggtgaa cgatgagggg ctcaaggacg atgccgccac
     3301 gcaacggctg ctcacactcg tccccgactt ctacgcgctc aactcctgcc ccgacttcat
     3361 cgaagacgag ggccactact tcggcaccgt cctgacggat gccgacaagc gcgcgctcat
     3421 cgagttcatg aagaccttct gaggagaatg ccatgccgaa cgatccgaac aatcctgact
     3481 tcgactacat cgtcatcggc tccggagccg gcggcggtcc cgttgcttgc aatctcgcca
     3541 aagcgggcta caaagtcggc ttgatcgagg ccggcatcga ggcgcagtcg caggcctacc
     3601 cggtgcccgt gtttcacacg tacgccagcg aagatcccga aatgtcgtgg gagtttttcg
     3661 tccgccacta ctccgacgaa gcgacatcga agcgcgactc gaaattcgtc gacgccgaag
     3721 atggcgtgtt ttatccgcgc gccgggacac tcggcggctg cacatcacat cacgccatga
     3781 tcacggtcta cggtcatggg agcgattggg actacatcgt cgacctcacc ggcgacgcga
     3841 gctggtcatc ggacaacatg cgctcctatt tcgagcggat cgagcgctgc acctacgcgg
     3901 gcccggcacc ggcagccgga caaccgaatc catcgcggca cggctacgag ggatggctga
     3961 atacgaccgg cccggagctc tcgctcgtct tcggcgacga cgcgctcctg aaaaccatcc
     4021 tcaccgcgat cctcggcgcc tggaaagaag gcatcagcgg cgttctcccc gatttgattc
     4081 cgcaggatcc caacgactgg cggacgccga agttcgaagg gatctgtttc gccccgttgg
     4141 caacgctgaa gggacgccgg ctgggaacgc gcgagttgat cgacaccacc cgcgccatct
     4201 atccgcagaa cctcgtcatc aggatgagca acctggccac gaagatcctc ttcgatgcga
     4261 acaagaaggc catcggcgtc gagtgctggg aagggcagca tctctacaaa gccgatccga
     4321 acgtcatcgc cgatccgcag gccggcgacg actatgaggt caagagttac tactgcaagc
     4381 gcgaggtgat tctcgccggc ggcgcgttca actcgccgca acttctgatg ctgtcaggca
     4441 tcgggccgaa ggatcaccta gcgaagttcg gcatcgattg catcctcgac cggcccggcg
     4501 tcggcatgaa tcttcaggac cgttacgaga tcgatgtctg cacggagatg aagaacgact
     4561 ggaagattct cgccaacgcc acgttcgcgc cgccgctacc caacgcgccc ggcgatccct
     4621 gctacatcga ctggcagaac ggcaaaggcg tctacgcgac caacggcgca gtcctcggcg
     4681 ttatcaagaa gtcgtcgccc gagc
//
LOCUS       DATLVW010000321         3012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_347131, whole genome shotgun sequence.
ACCESSION   DATLVW010000321 DATLVW010000000
VERSION     DATLVW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347131"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VNN25_09875"
     CDS             <1..592
                     /locus_tag="VNN25_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91876.1"
                     /translation="ATFGCAIVADFKRIDSYFDHSLAAAKRLHNLEISDDARTGYSYM
                     NFHLKQQSTSNGSRFVGEAAGFQDYLFGLGIRYALTSGSLAARSILEERDFDELWRHE
                     LGAKQETSLVNRFLYESGGNFGLSMFVRQASRAKDFHRYLSGWHRSSWWKKMLSPAVK
                     KFWKHDGRCLHKPGIHWCRSRDTEMKVPPLGPVETP"
     gene            606..1493
                     /locus_tag="VNN25_09880"
     CDS             606..1493
                     /locus_tag="VNN25_09880"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXH91877.1"
                     /translation="MTAEALVMPVSPTVDEAYALCRKIAHRYGSNFSVGFRFLPPVKR
                     KAVYAAYAWCRWADDIADEPSDSAESVLDRLAAWQSELEATYAGSPSHPITIALADAL
                     RHFAIPKSAFVALIDGCRQDMIKTRYQTFDELLQYCELVASSISDISLAIYGYRTPAA
                     LKYGRNLATALQLTNVTRDVGDDLDRDRVYLPQDELARFGVMESDLFARKENDAMRRL
                     IDFQIERASSYFRAAEPLVHELSFDARFPTLLMGGVYATVLARLRRNPLVVLRKRLSL
                     SRVEKIIVVARRLVRPRFV"
     gene            1529..2305
                     /locus_tag="VNN25_09885"
     CDS             1529..2305
                     /locus_tag="VNN25_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91878.1"
                     /translation="MARFLTALLLILGAAAASAADTPPPSVSVLVPIVGSVMGMNNVR
                     WKTDIDLFNDSRTEANVMLSLPTAPDQPFILVAIPPSSGQHFGDIVAESFGMSETLSP
                     LKIVTIDSARSVRVLATVYGVREADVSPPAPIAVTYGPSYGPLRTLNGLSFSDAFRTN
                     IGLANLGDHEALFTLAIQRVPGRNLAVTRLPVGASTLIHTAVQSLFPMITKGDDFVVI
                     VETSSPDTYTYASVIDNSTNETHFIQPAIGAPLIGQAQNQ"
     gene            2340..2504
                     /locus_tag="VNN25_09890"
     CDS             2340..2504
                     /locus_tag="VNN25_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91879.1"
                     /translation="MDEPEYLICLQCESPTYQFEYVNGKLATAICNICGADDISDFMT
                     EAELEESTGA"
     gene            2501..>3012
                     /gene="udk"
                     /locus_tag="VNN25_09895"
     CDS             2501..>3012
                     /gene="udk"
                     /locus_tag="VNN25_09895"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HXH91880.1"
                     /translation="MKTPVIIGIAGGTGSGKTTVARAIYDRVGKDRIEWISHDSYYRN
                     FDGYSPEQRHQINFDHPDSLETELLVRHLDVLAKGSSVDVPVYEFATHSRNPDKTHRV
                     EPRKVIIVEGILVLAEPELRKRIDIKLFVDTPADIRFLRRLTRDIESRGRSLTSVIDQ
                     YLTTVRPMHE"
BASE COUNT          574 a          996 c          860 g          582 t
ORIGIN      
        1 cgcgacgttc ggctgtgcga tcgttgccga cttcaagcgc atcgattcgt acttcgatca
       61 ctcgctcgcg gcggcgaaga ggctgcacaa tctcgagatt tctgacgatg cgcgcaccgg
      121 ctactcgtac atgaactttc acctgaagca gcaatcgacg tcgaatggct cgcgcttcgt
      181 cggcgaggcc gcgggatttc aggactatct cttcggcctc ggcatccgct acgcgctcac
      241 gagcggctcg ctcgctgcgc gcagcatcct cgaggagcgc gatttcgatg agctttggcg
      301 ccacgagctc ggagccaagc aggagaccag tctcgtcaat cgattccttt acgaaagcgg
      361 cggcaacttc ggcctctcga tgttcgtccg ccaggcctcg cgcgccaaag actttcatcg
      421 ttatctgagc ggatggcatc gcagcagttg gtggaagaag atgctctccc ccgccgtcaa
      481 gaagttctgg aaacacgacg ggcgctgcct gcataaaccg ggcatccact ggtgccgttc
      541 gcgcgacacg gagatgaagg tgccgccgct cggccccgtc gaaacgccat gactgccgag
      601 gccctatgac tgctgaggcg ctggtaatgc cggtctcgcc gaccgtcgat gaggcatatg
      661 ccctctgccg caagatcgcg caccgctacg gatcgaactt ctcggttgga ttccgcttcc
      721 tgccgcctgt gaagcggaaa gccgtttacg ccgcctacgc gtggtgccgc tgggccgacg
      781 acattgccga cgagccgagt gactccgcgg aatccgttct cgatcggctg gccgcgtggc
      841 aaagcgagct ggaggcgacg tatgccggca gtccgtcgca tccgatcacg atcgcattgg
      901 ccgacgcgct ccggcacttc gccatcccga agagcgcgtt cgtcgcactg atcgacggct
      961 gccgtcagga catgatcaaa acgcgatacc agacattcga tgagttgctg cagtactgcg
     1021 agctcgtggc gtcatcgatc tccgacatct ccctcgccat ctacggctat cggacgccgg
     1081 cagcgctcaa gtacggcagg aacctggcca cagcgcttca actgacgaac gtgacccgcg
     1141 acgtcggcga cgaccttgac cgcgaccgcg tctatctccc acaggacgag ctggcgcggt
     1201 ttggcgtgat ggaatctgat ctcttcgccc ggaaggaaaa cgacgccatg cgccgtctga
     1261 tcgacttcca gatcgagcgc gcatcaagct actttcgcgc cgctgagccg ctcgttcacg
     1321 agctctcttt cgatgcccgt tttcccacgc tgttgatggg cggcgtctac gcgacggtgc
     1381 tcgcccgact tcgccgtaat ccgctcgtag ttctgcggaa gcggctgtct ctcagccgtg
     1441 ttgagaagat catcgtcgta gcccggcgtc tcgttcgccc ccgcttcgtt tgaaacgtgc
     1501 gacgatcttg ctacgtctta gatacgacat ggctcgtttc ctcacagcgc ttctcctgat
     1561 cctcggcgcg gccgccgcga gtgcagccga cacgccgccg ccgagcgtca gcgtgctcgt
     1621 tccgatcgtc gggagcgtca tggggatgaa caacgtccgc tggaagaccg acatcgatct
     1681 cttcaacgac agccgtaccg aagcgaacgt gatgctgtcg ctgccgacgg cgcccgatca
     1741 gccgttcatt ctcgtggcca tcccgccgtc ctcggggcag catttcggag acatcgtcgc
     1801 cgaatcattc ggaatgagtg agaccctctc gccactgaag atcgttacga tcgacagcgc
     1861 gcgctccgtc cgcgtcctgg ccacggttta cggcgttcgc gaagcggacg tctcccctcc
     1921 ggcgccgatc gccgtgacct acggacccag ctatggtcct ctccggacgt tgaacggcct
     1981 ctccttcagt gacgcatttc gcaccaacat cggcctggcc aacctcggcg atcacgaagc
     2041 gctcttcaca ctggccattc aacgagtgcc aggccgcaat ctggctgtga cgagactccc
     2101 cgtcggcgcg agcactctga tccacaccgc ggtgcaatcg ctctttccga tgatcaccaa
     2161 gggcgatgac ttcgtcgtga tcgtcgagac cagttctccc gacacctaca cctacgcctc
     2221 ggtgatcgac aactcgacga acgaaacgca cttcattcaa cccgcgatcg gagctccgtt
     2281 gatcgggcaa gcgcagaatc agtagagaac gcgcgcctgg ttctgtattc ttcgcgttca
     2341 tggacgagcc cgagtacctc atctgcctgc agtgcgagag tccgacctat cagttcgaat
     2401 acgtgaacgg caagctggcc acggccattt gcaacatctg cggcgctgac gatatctccg
     2461 acttcatgac cgaagcggag ctcgaagaga gcaccggagc atgaagactc ccgtcatcat
     2521 cggcatcgcc ggcggaaccg gatcgggaaa gactacggtg gcgcgggcca tctacgaccg
     2581 cgtcggaaaa gaccggatcg agtggatctc gcacgactcg tactaccgca acttcgacgg
     2641 ctactcgccc gagcagcggc atcagatcaa cttcgatcac cctgactcgc tggagaccga
     2701 actgctcgtg cggcatctcg acgtgctcgc gaagggatca tcggtcgatg tccccgtcta
     2761 cgaattcgcg acgcactcgc gcaatcctga caagacgcac cgcgtggagc cgcgcaaagt
     2821 catcatcgtc gagggcatcc tcgtgcttgc cgaaccggag ctgcgcaagc gcatcgacat
     2881 caagctcttc gtcgacacgc cggccgatat ccgcttcctg cggcgcctca cgcgcgacat
     2941 cgaatcgagg ggaagaagcc tgacgtccgt gatcgatcag tacctgacga ccgtccgtcc
     3001 gatgcatgag ga
//
LOCUS       DATLVW010000322         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_95237, whole genome shotgun sequence.
ACCESSION   DATLVW010000322 DATLVW010000000
VERSION     DATLVW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95237"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..930)
                     /locus_tag="VNN25_09900"
     CDS             complement(70..930)
                     /locus_tag="VNN25_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH91881.1"
                     /translation="MSRLVLVVDDEEAIVSGLMCLLEDEAIKSAGAFDRLSAEALMET
                     TFYAVVVADLRLHTVEEGLELLDGIRRISPRSRVVTLTGYSTPEMVSEVLRRGSTMVI
                     HKPAVGEEIIAAVADLLAEVEKLADAQATLDLEQLHLGLRRLLHSIPRKRYGLSAEEA
                     EEVVQQAWLLFLEKRNLIRLAKPWLIGTVANLCKRQIGVTVRRRETFDSDDTLASLPD
                     KRAISPDTAIALRQALSALDDQSRKLCVLIAVEGLAYDEVSKRLGLPLGSIGPMYLRA
                     KSKMRMQMAA"
     gene            complement(1074..1451)
                     /locus_tag="VNN25_09905"
     CDS             complement(1074..1451)
                     /locus_tag="VNN25_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91882.1"
                     /translation="MSPDPSLKRALVIDDDDHIRELLQTLLETAGFEVETLRDGIDAV
                     ELKKDYHVILLDMKMPIFDAGRLTDYWKMTDPAILRRVILLSGYSRLASDRDFGTFAS
                     IAKPFDYLDLLSTVDECVRQTPA"
     gene            complement(1448..>3152)
                     /locus_tag="VNN25_09910"
     CDS             complement(1448..>3152)
                     /locus_tag="VNN25_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH91883.1"
                     /translation="STWGFAAYMQRMRRDGFADGIMNIVTRDGRKRTWKYRNALEIDE
                     HSVSVILGVARDVTECEEAFQAVLRSEEYFRSIVENVSDIIAIMELDGSLRYGSPSVC
                     RVLGYSRRALRGMRGLDLIHQDDVTQATSYLARQIADPAAAQTIELQVRHANGAWRCF
                     EIMATNLVKKGRTSAIVLNARDITGRKFLEAQLTQANRLGALGRLAATVAHEFNNVLM
                     GMQPFAELMQRPDAKPDLIAKGAGYIANSIQRGRRIVLDILRFAQPYQPVVAAVDLHE
                     WWETFAPEAENVLGNVISIVSAIPRGLYATADRMQISQVLANLVANARDAMPDGGLLT
                     VEANDLGADASFSFGVVPHPERFVQISISDTGKGMARDVMERVFEPLFTTRQSGGTGL
                     GLAVAHQVMTQHDGYIFVESELGQGSTFHLFLPKAAPPARKKSGQTAANRAPASRRVL
                     LIDDEQMIVEGIAALLEVDGIEVESIGSGFEAAETIARFHPDLVVLDLGLPGMNGSEV
                     YAHIREIDRLLPVIFASGHGDGQLLHDSFKDPHTRFLQKPFEVTDLLEMMVDIESEGT
                     S"
BASE COUNT          580 a          959 c          990 g          623 t
ORIGIN      
        1 ggctccggca gtctcaggat gacaccaatc tcgattggac gaaactacgg cgcgaacttg
       61 ccctcgccct caagccgcca tctgcatgcg cattttcgat ttggcgcgga ggtacatcgg
      121 gccgattgag ccgaggggga ggccgaggcg tttgctgact tcgtcgtagg cgaggccttc
      181 gacggcgatc aggacgcaga gtttgcgcga ctgatcgtcg agggcggaga gggcctggcg
      241 gagggcgatg gcggtgtcgg gagagatggc gcgcttgtcg ggaagggacg cgagcgtgtc
      301 gtcgctgtcg aacgtttcgc ggcggcggac ggtgacgccg atctggcgct tgcagagatt
      361 cgcgacggtc ccgatgagcc acggcttggc caatcgaatc agatttcgtt tttcgagaaa
      421 cagcagccat gcttgctgca ccacctcttc cgcttcttcc gcgctcaggc cgtagcgttt
      481 gcgggggatc gagtgcagaa gtcttcgcag gccgaggtgc agttgctcga ggtcgagagt
      541 cgcctgggcg tcggcgagct tttccacttc cgccagcagg tcagcgacgg cagcgatgat
      601 ctcttctcct accgccggtt tatggatcac catcgtcgag cctcgccgca agacctcgga
      661 gaccatttcc ggcgtgctgt aaccggtcaa cgtgacgacg cgcgagcgcg ggctgatccg
      721 ccggattccg tcgagcaatt ccagaccctc ctccaccgtg tgcaaccgga gatcggcgac
      781 gaccaccgcg tagaaggtcg tctccatcag cgcctccgcc gagaggcggt cgaatgcgcc
      841 ggcgctcttg atcgcttcat cttccagcag gcacatcagg ccggacacaa tcgcttcttc
      901 gtcgtcgaca accaggacca gtctgctcat gttcgcttcc tctggtggac caaagagcag
      961 acggcatacc gagcagagga ttacggcaaa tcacttgaaa tcagtcactt atgcgaaaca
     1021 ccgctttgac gcatgcgtat ggaaagcgga cacccgctct gaaaccggcc acatcacgca
     1081 ggcgtttggc gcacgcactc gtcgacggtc gaaagcagat cgaggtagtc gaacggtttc
     1141 gcaatgctgg cgaacgttcc gaaatcgcgg tcggacgcca gcctggagta gccgctgagc
     1201 aggatgacgc ggcgcaggat cgcgggatcg gtcatcttcc aataatcggt gaggcggccg
     1261 gcatcgaaga tcggcatttt catgtcgagc agaatgacgt ggtagtcctt cttcaactcc
     1321 acggcgtcga tgccatcgcg caacgtctcc acttcgaatc cggccgtttc gagaagtgtc
     1381 tgcagcagct cgcgaatgtg gtcgtcgtcg tcgatgacca acgcacgttt caatgatgga
     1441 tccggactca tgacgtacct tccgactcga tgtccaccat catctccagc agatccgtga
     1501 cttcgaacgg cttctgcagg aatctcgtgt gcgggtcttt gaaggaatcg tgaagaagct
     1561 gaccgtcgcc gtggccgctg gcgaagatga ccgggagcaa gcggtcgatc tcgcggatgt
     1621 gcgcatagac ctcgctgccg ttcatgcccg gaagcccgag gtcgagcacg accagatcgg
     1681 gatgaaagcg ggcgatcgtc tccgctgcct cgaaaccact gccgatggat tcgacttcga
     1741 tgccgtctac ctccagcagc gccgcgatgc cctcgacgat catctgctcg tcgtcgatca
     1801 acagcaccct gcgggatgcc ggcgcccggt tggcggcggt ttggccggac ttttttctcg
     1861 caggtggtgc cgctttcggc aggaagagat ggaacgtgct gccctggcca agctcgctct
     1921 cgacgaagat gtagccatcg tgctgcgtca tcacctgatg cgcgacggcg agcccaagac
     1981 ctgtgccgcc gctctgtctt gtggtgaaga gcggctcgaa gacgcgctcc atgacgtctc
     2041 gcgccatgcc ctttccggtg tcgctgatcg agatctgcac gaaccgctcg ggatgtggca
     2101 ccacgccgaa cgagaacgac gcgtcggctc cgaggtcgtt ggcttcgacc gtgagcagtc
     2161 cgccgtcggg catggcatcg cgggcattcg ccacgaggtt cgccagcacc tgcgaaatct
     2221 gcatgcggtc ggccgtcgcg taaaggcccc gcggaatggc cgaaacgatg gagatcacgt
     2281 ttccgagaac gttctccgct tccggagcga acgtctccca ccactcatga aggtcaaccg
     2341 cggcgacgac tggttgatac ggctgtgcga agcgaaggat atcgagcacg atgcgcctgc
     2401 cgcgctggat ggagttggcg atgtaaccgg cgcctttggc gatgagatcg ggcttcgcgt
     2461 ccgggcgctg catcaactcc gcgaacggct gcatccccat cagcacattg ttgaactcgt
     2521 gcgcgacggt ggcggcgagg cggccgagtg cgccgaggcg gttggcctgc gtcagttgcg
     2581 cctccaggaa tttgcgtccg gtgatgtcgc gggcgttcag cacgatggcc gaggtccgcc
     2641 ccttcttgac cagattcgtg gccatgatct cgaagcagcg ccaggcaccg ttggcgtgac
     2701 ggacctgcaa ttcgatggtc tgggcagcgg ccgggtccgc gatctggcgc gccaggtagc
     2761 tcgtcgcctg tgtcacatcg tcctgatgga tcagatcgag ccctcgcatg ccacgaagcg
     2821 ctctcctgga gtagccgaga actctgcaga ccgagggact gccgtagcgg aggctgccat
     2881 cgagctccat gatggcgatg atgtcggaga cgttctcgac gatcgagcgg aagtactctt
     2941 cgcttcgcaa taccgcctgg aaggcttcct cacattcggt cacatcgcgt gcgacgccca
     3001 gtattacgga cacggaatgc tcatctatct cgagcgcatt gcggtacttc caggtccgct
     3061 ttctaccgtc gcgcgtgacg atattcatga tgccgtctgc gaagccgtcg cgcctcattc
     3121 tctgcatgta ggccgcgaag ccccacgtgc tg
//
LOCUS       DATLVW010000323        13792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_262385, whole genome shotgun sequence.
ACCESSION   DATLVW010000323 DATLVW010000000
VERSION     DATLVW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262385"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VNN25_09915"
     CDS             complement(<1..248)
                     /locus_tag="VNN25_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91884.1"
                     /translation="MRLTSACFFPLLVLSIGCGKLPTAPAEGHAALTANEVAASTGRR
                     RVTSPYNRLVPPCAAPAPLHAAAPQERVPGYIFRYRDGT"
     gene            complement(360..1400)
                     /locus_tag="VNN25_09920"
     CDS             complement(360..1400)
                     /locus_tag="VNN25_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91885.1"
                     /translation="MLKVKQLGVAAAIALFAGAAMASNFRAADQVYIPAAGHIVGANA
                     TFVSDVFISNLSDDSVDVSVIYATGNTTATAVQTPFPKVLTLAPRERRELLDFMSNTL
                     HLSSAFGQVIFNGCLKDADCTPNATTGLNSNYRNISVESRIYSFTTGANQATAPTNGQ
                     LFSGIPWYNFVSESASGAGLDKIFITGLRNTPQYKTNVGFVNASQFSTTTLTAKLYSG
                     SAPSTVIATANFTLPPLGFQQTTLGSSLFGSAVPQSATSTNLFITVEQGSTQKVVGGP
                     NDADANGCPTGCPAFFAYGSQLDNSTQDATTLESQYSLPLTNNAITCIYSPDVAACKS
                     GTTIHRAAKHQF"
     gene            1594..2526
                     /locus_tag="VNN25_09925"
     CDS             1594..2526
                     /locus_tag="VNN25_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXH91886.1"
                     /translation="MKVTFVDQDEGERLDRALATHLPEHSRSFLAGLIENGHVTLDGK
                     AAGKPSVRVAAGATAVLDIPPLLPATAQSQELALTVIFEDEDLIVIDKPPGLVVHPSA
                     GHADGTLVNALLFHVRDLSGIGGELRPGIVHRLDKDTSGVMVIAKNDVAHRKLSAAWG
                     TERVRKEYIAIVYGTPRESQGVIDAPIARDPHNRKRMAIVTGGRRAVTEYGVIESLRH
                     SSVVRCVLHTGRTHQIRVHLKSIGHPIIGDPVYSGPQWRGIPDKKLQKAIASFQRQAL
                     HAAVLVIPHPRTDKQMTFSGGLPEDMVRLIEALR"
     gene            complement(3053..4474)
                     /locus_tag="VNN25_09930"
     CDS             complement(3053..4474)
                     /locus_tag="VNN25_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HXH91887.1"
                     /translation="MERFSGLIGFVGILAIAYLLSTNRRAIRWKTVFWGLTLQIVTAI
                     AVLRGTQIADLFRKFMPAINPWIAALIFVVLTIIIVQITKRMEHGTPRTGVWGVYLVI
                     SAVLFLAYNLLAFLFQNLKDVVTHLISYTAEGSKFVFGPLGDPANTNIGFVFACQVLP
                     TIIFIASIFAVLYYLGVMQVVVRFFAKLMSRFMGASGAESLSVAASIFMGQTEAPLTI
                     RPFIDGMTMSELMTIMTAGMAHISGGIMAAYVLVAKVDVIHLLTAVIMTAPGAIMMAK
                     MIVPETEKPATGADVEVVVPKQDVNVIDAAGRGAIEGLHLSLNVAGMLIAFIALVALV
                     NGVFGYAHGMVWWVPESLDKLLGWIFSPIAWAMGVSWKDSLTVGNLLGTRMVLNEFVA
                     FVNLGKPGVGSSLDPRSFVIVTYALCGFANFSSIAIQIGGIGSLAPSRRGDLARLGLR
                     AMLAGTLANFLTATIVGILYVAK"
     gene            4645..5463
                     /locus_tag="VNN25_09935"
     CDS             4645..5463
                     /locus_tag="VNN25_09935"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXH91888.1"
                     /translation="MIEAINEAVRFVRSRSSMQPRVGVILGSGLGDVVDALEVETAIP
                     YSEIPGARASTVVGHQGRMILAHVEGTPVVVMQGRVHFYEGYEMAEVMFLSRVIGRLG
                     IEKLIVTNAAGGINTKFKAGDLMLISDHINMMGMNPLRGPNVEDLGVRFPDMSEAYPE
                     SLRDIAKDVARELGQLIQEGVYLALSGPTYETPAEIRAFRHLGADAVGMSTVPEVIAM
                     SQMQIPVLGISCITNMAAGILKQKLTHQEVMDTTARVQKDFTALVLGCLQRFAA"
     gene            complement(5624..6259)
                     /locus_tag="VNN25_09940"
     CDS             complement(5624..6259)
                     /locus_tag="VNN25_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91889.1"
                     /translation="MRVRAISLIATILVTTSLFAADPTKPVVQKLEQLASTFCNQTYA
                     LCIKAPCSPIVTHNKKDGTYSIEQADCVCDVLSGWSMGPGACDKRAPETVNGRTFLIS
                     TYSNFFNKTNLTLSCPSSTQMWAWCYGSPCAVDEKNPSKAVCTCPIKISPSMTLGGEC
                     RQEACNSIWSAATPANDAYANNTFFNYMTEHDLQPPPNPPAKDCPTTTSSN"
     gene            complement(6445..7173)
                     /locus_tag="VNN25_09945"
     CDS             complement(6445..7173)
                     /locus_tag="VNN25_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91890.1"
                     /translation="MKGKLQVVLEKMDHGHHVLRACAKNAVLRMYEKFSTFLRIEALS
                     NNLGDFGLRKGLDNLDHVRKTLTAVTDRFANFEAQALNVQVDFPLFQRLALPITSGRT
                     RVAGIKIHDTRMIRLMEVLLHAGTKIGGWRSTDIHQAILKAFSLQPASYSLTQLRYDV
                     RKMKAHGLVERDGKRYVYRLTEKGNKVALMFILFHERVAGPLANNLFSDHPPTPDFPP
                     PTRIEKAYRRANKSIQQIIDLLAA"
     gene            7315..7821
                     /locus_tag="VNN25_09950"
     CDS             7315..7821
                     /locus_tag="VNN25_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91891.1"
                     /translation="MNGVLAGEVDLNVLGNDGPLTLDSLARRAEKLKLRTPWLTIFLD
                     ITADDKTALQLLDEAWSRWRVRVAFTADLGAEKWITDSERQDVAVHRVEEFTPRQIRR
                     FLEKRDIDWRQIPSFLIEPPYERTLTPCSIRQSRRPKRTIKRRVSRTSTEAEPFLRTP
                     SAGYNARP"
     gene            7818..10052
                     /locus_tag="VNN25_09955"
     CDS             7818..10052
                     /locus_tag="VNN25_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH91892.1"
                     /translation="MKNCIAALLLFTAFSGFAQDIKTTPQPKPTIRELTLENVFDPKA
                     KVSFGGTPQGGFVWLDDKAFAWPRTEEGKVVEQEVIESETGNKRVLFNAAKLEAAAKK
                     ITGVTAEEATGFTKQRNWNFSPNKKSVLLTIANDVYLYAFDSDSMTRLTSSPGAKEEV
                     TFSPDGRSVSFVRGNNIYVVDVATQRERQLTTDGNENIQNGILDWVYQEEVYGRGNFR
                     AYWWSPDSSYIAYLQLDERPVKHFAVVDHIPYQQNVESELYPKAGAPNPGVKLFTVRA
                     AGETPHEVSTESYSSGDFLIVLVDWSPDSAKVMYQVQNREQTWLDLDAAEVRGGGAPK
                     TLFRDSSKAWTEPTGNPVWLKDGSFLWLSERSGFKHLYQYAADGKLIRQITTGPWEVR
                     TLHGIDNANDWIYFSGTERSVLGTDLYRVHLDGSGLKRLTDAPGQHTVNFNPSLTAYL
                     DTFSDAATPPSTSLHRHDGTSVRVVDANEAPLMRSLNIAKPEFVQVKTRDGFVMEAEM
                     IKPPNFDPTKKYPVYEHTYSGPHAQQVRNAWGASTYLWHQFLAQHGVIVWICDNRSAS
                     GKGMESAWTSYKRMGEGELRDLEDGLKWLESQPYVDSSRVLLSGWSYGGFMTSYALTH
                     STMWSAGISGGTVTDWRDYDSIYTERVMLMPQNNPEGYKATAPRWAAKDLHGSILLLH
                     GTIDDNVHMQNTIQFIYELEKANKPYQLQVYPKSRHGVVDPALVAHMRAAMWKFIEEN
                     LGVK"
     gene            10218..10460
                     /locus_tag="VNN25_09960"
     CDS             10218..10460
                     /locus_tag="VNN25_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91893.1"
                     /translation="MAKQMTIRGVPEDVAGKLRRLSKRRGRSVNTTVLEILSQAVGSD
                     ARRERLARYATWTTDDLAQVEEAVAAQRVIDESLWR"
     gene            10466..10864
                     /locus_tag="VNN25_09965"
     CDS             10466..10864
                     /locus_tag="VNN25_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH91894.1"
                     /translation="MQRLVMDTSAYSHMRMGHPELTDLLASAEALLIPVTVLGELEGA
                     FELGSRASVNRRVLTEFLSEPFVSILPTTPDVARRYGQIFARQRRAGRPVSVNDMWIA
                     ASAIDSGGHLVTFDKDFGRIEALDCTILAP"
     gene            11021..12079
                     /locus_tag="VNN25_09970"
     CDS             11021..12079
                     /locus_tag="VNN25_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXH91895.1"
                     /translation="MFESATPATSTPRFKPSFPFKLPPLRGGFVRFLLRASVILVPII
                     FMRACFITYVPLDQIGLRQISFGMSKGLQKQLVEPGYRRAISGYETIRTFPRNIQAVE
                     FTNDESERGADHRTVAAINVPTVDGYPVAVDVTVLYRIADPFLVVSKFGFGRGYEDNV
                     VLRFTDPAIKQHLGELRAEEFYRDQRIAKVRDLKRELSEKFRQNGLELADVLIRQYDY
                     PDTFQQLTEQKKIQDQSVLTNRALAKQAEVDTRLKQTTAEGQNSINVRSAEFQAQITG
                     LNAQKDFYERSKHAEADLLVKSAEAQGTEEINKAMEGAGSDKLLRLRKGLAILNGIQG
                     PIYISEDPTDIGKLTGKH"
     gene            12087..13094
                     /locus_tag="VNN25_09975"
     CDS             12087..13094
                     /locus_tag="VNN25_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXH91896.1"
                     /translation="MKIRLIALLAIAPLFAACTPHTTQATEVGVRFNKITQSTEISDP
                     GATYFFAPVINDWATYDTSTQNLVMSAKATEGDRKEKDDLRFKTRDGNDIETDVTVRW
                     RIDPTKAAYIWRTVAPNTVALKERLVRPLARAYVRDVLNRLDSEEYYNPDLRFAAATD
                     ATKVMAVQLKPYGVIVEQVILGDFSFKPDYQNLINKRKEAEKQAEKLESQILATLESN
                     KANLQSKVADLTEQLTHSQGDFEQAKRAADAYVVRKQQDAQATLSEKSAVAQGIKKER
                     AALNGSAGDAYVSLQLIDALQKKEIRQIPKLPNGNVIIDGNKLLEQLGVIQYQQKKND
                     Q"
     gene            13094..13684
                     /locus_tag="VNN25_09980"
     CDS             13094..13684
                     /locus_tag="VNN25_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91897.1"
                     /translation="MLNIAGSEYDYAQVVYVVLQECEHRRRGFEEEEFDQDIRACAAE
                     KMAEIKAAYDEFGGSAAYWEALDKEVEEVVIPRYLVASREMTELERTNFMIWRGGDLA
                     ARFVFALIGLFIGSIIIALPFIPIFEDMFALALMAAGFLYPDLKRFMFERRYTKVLNH
                     IVSDSAGYQENARLHYMTSQDIQKAFEPGNPRQLPP"
BASE COUNT         2875 a         4110 c         4249 g         2558 t
ORIGIN      
        1 gtgccgtcgc gatacctgaa gatgtagcct ggtacccgct cctgcggcgc ggccgcgtgc
       61 aggggcgccg gcgcggcgca cgggggcaca agacggttgt aggggctcgt cacgcgccgg
      121 cgtcccgtgg aagccgcgac ctcattggcg gtcagcgccg cgtgaccctc ggctggcgcg
      181 gtcggcagct tgccgcaacc tatcgagaga actaagagtg ggaagaagca tgcagaggtc
      241 aagcgcattc tgtcagtagc taagcatgtt acgccggacc gcattccgat cttcgtgaca
      301 taaaaaaacg gccgcgccct gaggcgcggc cgtcgattca aaccgagtct aaattgctct
      361 tagaactgat gcttcgccgc acgatggatg gtcgttcctg acttgcaggc ggcgacgtcc
      421 gggctgtaga tgcacgtgat ggcgttgttc gtgagcggaa gcgaatactg cgactcgagc
      481 gtcgtggcgt cctgcgtcga gttgtcgagc tgcgagccgt acgcgaagaa cgccgggcaa
      541 cccgtcgggc aaccattggc atcggcatca ttcggtccgc cgacgacttt ctgcgtacta
      601 ccctgctcga cggtgatgaa caggttggtc gaggtcgcgc tctgggggac ggcacttccg
      661 aacagactgg agcccagcgt ggtctgctgg aagccgaggg gcggaagcgt gaagttggct
      721 gtagcgatga ccgtgctggg agcggagccg ctgtagagct tcgcggtcag cgtcgtcgtc
      781 gagaactgcg aggcgttcac gaatccgacg ttcgttttgt attgcggcgt gttgcgaagg
      841 ccggtgatga agatcttatc cagaccggcg ccggacgcgc tctccgaaac gaagttatac
      901 caggggatgc cggagaagag ctgaccattg gtcggcgccg tcgcctggtt cgctccggtc
      961 gtgaacgaat agatgcggct ctcgacgctg atgttgcggt agttcgaatt caggccggtc
     1021 gtcgcattcg gcgtgcagtc cgcatccttc aggcagccgt tgaagatcac ctgtccgaac
     1081 gcgctgctga ggtggagcgt gttcgacatg aagtcgagca gctcacgacg ttccctcggt
     1141 gccagtgtga gcacttttgg aaacggagtc tgcacggcgg tggccgtcgt gttgccggtg
     1201 gcgtaaatca cggaaacatc gaccgagtca tcgctcaggt tcgagatgaa gacatcggag
     1261 acgaaggtgg cattcgcgcc tacgatatgg ccggctgccg ggatgtagac ctggtcggca
     1321 gcgcggaagt tgctggccat cgccgcaccg gcgaacaacg cgatcgctgc agccacgccc
     1381 aactgtttca ctttcaacat attctcccct tttgctacga gtatttggaa tcagactgcc
     1441 tcgatcgaaa agtgcgtacg cattctaatc gactttgcca gccaaaaaag acacgacgaa
     1501 acttgatacg aaccaaagaa gcagaacgtc acacttcgac gctcagggct ttcgccaaag
     1561 aactatgcga ttctagcatg atatgaggga ccgttgaagg ttacgttcgt ggatcaggat
     1621 gagggggaac ggctcgaccg ggctctggca acgcatcttc ccgagcatag ccggagtttt
     1681 ctggccgggc tcatcgagaa tggccacgtc acgctcgacg ggaaggcggc cggcaagcct
     1741 tccgtgcgcg ttgcggccgg ggcgactgct gttctcgaca tccctcctct cctgccggcg
     1801 accgcgcaat cccaggaatt ggctctcacg gtgatcttcg aagatgagga tttgatcgtc
     1861 atcgacaagc cgcccggact cgtcgtccac ccctcggcgg gacatgcgga cggaacgctg
     1921 gtgaacgcgt tgctttttca cgtgcgcgac ctcagcggta tcggcggcga gcttcggccg
     1981 ggcatcgtgc accgcctcga caaagacacg agcggcgtga tggtcatcgc gaagaatgac
     2041 gtcgcgcatc gcaagttgag cgcggcctgg ggaacggaac gcgtgcggaa ggagtacatc
     2101 gccatcgttt acggaacccc gcgcgaatcg cagggcgtga tcgatgcgcc catcgcccgc
     2161 gatccgcaca atcgaaaacg gatggcgatc gtcaccggcg gccgccgcgc ggtcacagag
     2221 tacggcgtga tcgaatcgct gcgccactcc tccgtcgtcc gctgcgtcct gcacacaggc
     2281 cgcacgcacc agatccgcgt tcacctcaaa tcgatcggcc atcccatcat cggcgacccc
     2341 gtctactccg gaccgcaatg gcgaggcatc cccgacaaaa aactccagaa agcgatcgca
     2401 tcgttccaaa ggcaagcgtt gcacgcagca gttctggtga ttccgcaccc gagaacggac
     2461 aagcagatga cattctcggg cggtttgccg gaggacatgg tgaggttgat cgaagcgctg
     2521 cgttagcccg catttctcac acccctcccc tcggctctct ccaatcgagc gccttctccc
     2581 cccgcgcttt ttgcgggggg agaaggtggc cgaaggccgg atgagggggg tgccagtgcg
     2641 caggcgatta accacgcctc gtccgaactg cttgaaaacg cggcacatgc gcggatgtcg
     2701 tacgcgccgc gctcgcgata atgcggcgtg accggtgcgc acgtgcgcgc cgagggtgca
     2761 acgctgtgac acggtgagtg cgcacgtgcg cgccgagtgt gccccactgc gtcgcagtag
     2821 gagcgcatgt gcgctccggc ggtgtcacac tgcgtcgcag taggagcgca cgtgcgctcc
     2881 gggagtgcca cactgcgtcg cagtaggagc gcatgtgcgc tccgggagtg tcccactgcg
     2941 tcgcagtagg agcgcatgtg cgctccgagc gtgcaacctt gcgtcgcggc aggtccgtac
     3001 gtgcgcaccc agggcgcaac cgggagatac tcaggaactc tgcagcactc ccctacttcg
     3061 ccacgtacag gatccccacg atcgtcgccg tcaggaagtt cgccagcgtt ccggccagca
     3121 tcgcgcggag tcccaaccgc gcgagatcgc cgcgtcgcga tggggcgagc gagccgatgc
     3181 cgccgatctg gatggcgatc gaggagaagt tcgcgaagcc gcacagtgcg taggtaacga
     3241 tgacgaagct gcggggatcg aggctcgacc cgacgccggg cttgccgagg ttgacgaagg
     3301 cgacgaactc attcagtacc atgcgcgtgc cgaggagatt gccgaccgtg aggctgtcct
     3361 tccacgacac acccatcgcc catgcgatcg ggctgaagat ccagccgaga agtttgtcga
     3421 ggctttccgg aacccaccac accatcccgt gcgcgtagcc gaacacgccg ttcaccaacg
     3481 ccacgagcgc gatgaaggcg atcagcatgc cggcaacgtt taaggaaaga tgcagacctt
     3541 cgatggcgcc gcggccggca gcatcgatca cgttcacatc ctgcttcgga acgacgactt
     3601 cgacatccgc accggtggcc ggcttctccg tctccggcac gatcatcttc gccatcatga
     3661 tcgcgcccgg cgcggtcatg atcaccgcag tgagcaggtg aatgacgtcg accttcgcca
     3721 cgagcacgta cgccgccatg atgccgcccg aaatgtgggc catgccggcg gtcatgatcg
     3781 tcatcaactc cgacatcgtc atgccgtcga tgaacggccg gatcgtcagc ggtgcttcgg
     3841 tctgtcccat gaagatcgac gcggccaccg agagcgactc cgcgcccgac gcgcccatga
     3901 agcgcgacat cagcttcgcg aagaagcgca caaccacctg catcacgccg aggtagtaga
     3961 ggacggcgaa gatggaggcg atgaagatga tcgtcgggag cacctggcag gcgaagacga
     4021 agccgatgtt cgtgttggcc ggatcgccga gcgggccgaa gacgaacttg gagccctcgg
     4081 ccgtgtagct gatcagatgg gtgacgacgt ccttcagatt ctggaagagg aacgccagga
     4141 ggttgtaggc cagaaagaga acggcgctga tgacgagata gacgccccag acgcccgttc
     4201 gcggggtgcc gtgctccatc cgtttggtga tctgcacgat gatgatcgtg agcacgacga
     4261 agatcagcgc cgcgatccat ggattgatcg ccggcatgaa ctttcggaac agatccgcga
     4321 tctgcgtgcc gcgcaacacg gcgatggcgg tgacgatctg aagcgtcagg ccccagaaaa
     4381 cggtcttcca gcgaattgcg cgccggttcg tcgacagcag atatgcaatc gccagaatcc
     4441 ccacgaatcc gatcagcccg gagaatcgct ccattcacag ctcccgtcgt tgccgaattt
     4501 tgatgatgac cgcttggatg gcgggcatta tgcgggatcg agcgcgcggt tgcgaggttt
     4561 cgtattgcgg cgtggtttcc atctcgcctc gcgatccgct accgctatac tccgcgctcc
     4621 aagttcgtcc aaacgaggaa acaaatgatt gaagcaatca acgaagccgt gaggtttgtc
     4681 cgcagcagat cgtccatgca gccccgtgtc ggtgtcatcc tcggctccgg cctcggcgat
     4741 gtcgtcgacg ccctcgaggt cgagactgcg atcccctact ccgagatccc cggcgcgcgc
     4801 gcctcaaccg tcgtcggtca ccaggggcgg atgatcctcg cgcatgttga gggcacgccg
     4861 gtcgtcgtca tgcagggacg cgttcacttt tacgaaggct acgagatggc ggaggtcatg
     4921 ttcctctcgc gcgtcatcgg acgcctcggc atcgagaagc tcatcgtcac gaacgccgcg
     4981 ggtggcatca acacgaagtt caaggcgggc gatctgatgc tcatctccga ccacatcaac
     5041 atgatgggga tgaacccgct ccgcggtccg aacgtcgaag acctcggcgt ccgtttcccc
     5101 gacatgagcg aggcctaccc cgaatcgctg cgcgacatcg caaaagacgt tgcccgcgag
     5161 ctcggccaac tgatccagga aggcgtttac ctggcgctgt ccggaccgac ctacgagacg
     5221 ccggccgaga tccgcgcctt ccgccacctc ggcgccgatg ccgtcggcat gtcgaccgtt
     5281 cccgaggtca tcgccatgtc acaaatgcag atcccggtgc tcggcatctc ctgcatcaca
     5341 aacatggccg ccggaatcct gaaacagaaa ctgacgcatc aggaagtcat ggacacgacc
     5401 gcccgcgtgc agaaagactt caccgcgctg gtgttgggat gcctgcaacg gttcgcggcg
     5461 taggcaggct gcggctgaaa cgaagctata cgtaggtcgg actctccagt ccgacatcga
     5521 tatacgtagg tcggactctc tagtccgaca tcgcgttcca gtcggactgg agagtccgac
     5581 ctacgtaaca ctcgcttccc acggagcaga aacgactgag cgatcagttg ctcgacgtcg
     5641 tcgtcggaca atccttcgca ggaggattcg gcggcggctg aagatcgtgc tcggtcatgt
     5701 agttgaagaa cgtgttgttc gcgtacgcat cgttcgccgg cgtggccgca ctccagatgc
     5761 tgttgcatgc ttcctggcgg cactcccctc cgagcgtcat cgaggggctg atcttgatcg
     5821 gacaggtgca gacggccttc gagggattct tctcatcgac ggcacatggc gatccgtagc
     5881 accaggccca catctgggtc gagctcggac acgagagcgt caggttcgtc ttgttgaaga
     5941 agttcgagta cgtcgaaatg aggaacgtgc gcccgttcac cgtttccggc gctcgtttgt
     6001 cgcatgctcc cggtcccatc gaccatccgg acagaacatc gcaaacgcaa tcggcttgct
     6061 cgatcgagta ggtgccatcc ttcttgttgt gagtgacgat cggactgcac ggcgccttga
     6121 tgcagagcgc gtacgtctga ttgcagaagg tcgaggcgag ctgctcgagc ttttgcacga
     6181 ccggtttcgt gggatcggcc gcgaaaagtg aagtggtcac caggatcgta gcgatgagag
     6241 agatagcccg gacgcgcatg agacctcccg tacttcctga tcgattgtgg acagtgatga
     6301 cgctacttac atccgccatt cctgtcaagc gcggggtcag gtctaaagtc taaagtgccg
     6361 tagacgatcg ttgctaatgc gatgagctct cctcggcact ttagattctt ggcggtgaga
     6421 tctgagagat ttgctcgcag agtttcatgc cgcgagtagg tcaatgatct gctggattga
     6481 cttgttagcg cgacgatatg ccttttcgat tctggtgggc ggtgggaagt cgggcgtagg
     6541 cggatgatcg ctgaaaaggt tgttggcgag tgggccggcg acgcgctcgt ggaacaggat
     6601 gaacatgagc gcgaccttgt ttcctttttc ggtcagtcga tagacgtagc gcttgccatc
     6661 tcgctcaacc aggccgtgag ctttcatctt tcggacgtcg tagcgtagct gagtcaggga
     6721 gtacgaggcg ggctggaggg agaaggcctt caagatcgcc tggtgaatgt cggtggagcg
     6781 ccatccgccg atctttgtac cggcgtgaag gagaacctcc atgagccgga tcatgcgagt
     6841 gtcgtggatc ttgataccgg ctacgcgggt acggcctgag gtgatgggca gggccagtcg
     6901 ctggaagaga gggaagtcca cctggacgtt gagtgcctga gcctcgaagt tggcgaagcg
     6961 gtcggtgaca gcggtcagcg ttttgcggac gtggtcgagg ttgtcgaggc ccttgcgtag
     7021 cccgaagtcg ccgagattgt tgctcagggc ttcgatacgc aagaaggttg agaacttctc
     7081 atacatccgc agcaccgcgt tcttggcaca ggcgcggagg acgtggtggc cgtggtccat
     7141 cttctctagg acaacctgca gcttcccttt caaacgtttg tttttgcgaa agtcgaagat
     7201 ctggatgatc ttgtcgggaa caaggcgaac gacgccgata tcgcaggaac gctcaaagag
     7261 cttgtggatg ggagaggttc tccggaagat gagattgcgg cagtactcga cctgttgaat
     7321 ggagtactgg cgggtgaggt tgacctcaac gtcctcggca atgacggacc cctgacgcta
     7381 gacagtcttg cgcgtcgcgc agaaaagcta aaactcagaa caccgtggct gacaatcttt
     7441 ctcgatatca ccgccgacga taaaacagcg ctccagttgc tcgacgaagc ttggagtcgt
     7501 tggcgcgtcc gcgtcgcatt cacggctgat ctgggcgccg aaaaatggat taccgattcc
     7561 gagcggcaag atgtcgccgt acaccgcgtc gaggagttca cgccccgtca gatacggcgg
     7621 tttctcgaga aacgcgacat cgattggcgc cagatcccat ccttcctcat cgagccgccc
     7681 tacgagcgca cgcttacgcc ctgctcgata aggcagtcaa gaagaccgaa gaggactatc
     7741 aaaaggcgag tctcccgaac ttctaccgaa gctgagccct tcctgcgcac cccatcggcg
     7801 ggatataacg cgcgtccatg aagaactgca tcgccgctct acttctgttc accgccttct
     7861 ccggtttcgc gcaagacatc aagaccacgc cgcagccgaa gccgacgatt cgcgagctga
     7921 cgctcgagaa cgtctttgat ccgaaagcga aagtgtcgtt tggcggaacg ccgcagggtg
     7981 gcttcgtctg gctcgacgac aaagcgtttg cgtggcctcg gaccgaagaa ggaaaggtcg
     8041 tcgagcagga ggtgatcgag agcgagacgg gcaacaagcg cgtgctcttc aacgccgcga
     8101 agctggaagc ggcggcgaag aagatcaccg gagtcacggc ggaggaagcg accggtttca
     8161 cgaaacagcg gaactggaac ttcagcccga acaagaaaag cgttctgctg acgattgcga
     8221 acgacgttta cctctacgcg ttcgacagcg attcgatgac gcgccttacc tcctcgccgg
     8281 gtgcgaaaga ggaagtgacg ttcagtccgg acggacgatc tgtctcgttc gtgcgcggca
     8341 acaacatcta cgtcgtcgac gtcgctacgc agcgcgagcg gcagctcacc accgacggca
     8401 acgagaacat ccagaacggc atcctcgact gggtctatca ggaagaggtg tacggccgcg
     8461 gcaacttccg cgcgtactgg tggagtcccg actcgtccta catcgcctac ctgcagctcg
     8521 acgagcggcc ggtgaagcat ttcgccgtcg tcgatcacat cccctatcag cagaacgtcg
     8581 agtcggagct ctatccgaaa gcgggcgcgc cgaatccggg cgtgaagctc ttcaccgtcc
     8641 gggccgcggg cgaaacgccg cacgaggtga gcaccgagag ttacagcagc ggcgacttcc
     8701 tcatcgttct cgtcgactgg tcgccggaca gcgcgaaggt catgtaccag gtgcagaacc
     8761 gcgagcagac gtggctcgat ctcgacgccg ccgaagttcg tgggggcggc gcgccgaaaa
     8821 cgctctttcg cgattccagc aaagcatgga cggaaccgac cggcaatccg gtctggctca
     8881 aggatggatc attcctctgg ctcagcgagc ggagcgggtt caagcacctt tatcaatacg
     8941 ccgccgacgg gaaactcatt cgccagatca cgaccggacc gtgggaagtg cgcacgttgc
     9001 acggcatcga caacgccaac gactggatct atttctccgg cacggagcgc agcgttctcg
     9061 gcaccgatct ctatcgcgtc catctcgatg gcagcggtct gaaacgcctc acggacgcgc
     9121 ccggccagca cactgtcaac ttcaatccgt cgctgacggc gtatctcgac acgttctccg
     9181 atgccgccac gcccccttcg acgtcgctgc atcgccatga cggtacgagc gtgcgcgtcg
     9241 tcgacgcgaa tgaggcgccg ctgatgcgca gtctgaacat cgccaagccg gagttcgtgc
     9301 aggtcaagac gcgcgacggc ttcgtgatgg aagcagagat gatcaagccg ccgaatttcg
     9361 atccgacgaa gaagtatccg gtctacgagc acacgtactc cggtccgcac gcgcagcagg
     9421 tacgcaacgc atggggcgcg tcgacctatc tctggcacca gttcctggcg cagcacggcg
     9481 tgatcgtctg gatctgcgac aaccgcagcg ccagcggcaa agggatggag tcggcctgga
     9541 cgtcgtacaa gcggatgggc gaaggagagc ttcgcgatct cgaggatggg ttgaagtggc
     9601 tggagtcgca gccgtacgtc gattcgtcgc gcgtgttgct gagcggctgg agctacggcg
     9661 gcttcatgac gtcgtacgcg ctgacgcaca gcacgatgtg gagcgcggga atctctggcg
     9721 gcacggtcac cgactggcgc gattacgact cgatctacac cgagcgcgtg atgctgatgc
     9781 cgcagaacaa tccggaaggc tacaaggcga cggcgccacg ctgggcggcg aaggaccttc
     9841 acggcagcat cctcctgctg cacggcacga tcgacgataa cgtgcacatg cagaacacga
     9901 ttcagttcat ctacgagctg gagaaggcga acaagccgta tcagttgcag gtctatccga
     9961 agtcgcggca cggcgtggtg gatccggcgc tggtcgcgca catgcgcgcc gcgatgtgga
    10021 agttcatcga ggaaaacctc ggcgtcaagt agcgaccaga gatcagagac aagagacgaa
    10081 gagatcagga gcgcgacagt ctccctgatc ggtggctcag aaatcacaaa gttatcgttt
    10141 catctctgta cactctgagc ctctgtggtt gctgattctc ctcgggaatg caggcacaat
    10201 tgtgctagca tgcaggcatg gcaaagcaga tgaccatccg cggcgtcccg gaagatgtcg
    10261 ccgggaaact tcggcgactg agcaagcgtc gtgggcggag cgtgaacacc acggtgctcg
    10321 agattctcag ccaggccgtc ggttccgatg ctcgccgtga acggctggcg cgatatgcca
    10381 catggacgac tgacgacctc gcacaggttg aagaagcggt cgccgcccaa cgggtgatcg
    10441 acgagtcgct ctggcggtaa ccaccgtgca acggctcgtc atggacacct cggcgtactc
    10501 ccacatgcgc atggggcacc cggagttaac cgatctgctc gcgtcagccg aggccctgct
    10561 aattcccgtg acggtactcg gcgaattgga gggcgcgttt gaactgggct cgcgtgcaag
    10621 cgtcaaccgc cgcgttctga ccgagttcct ttccgagcct ttcgtttcga tcctgccgac
    10681 gactcccgac gttgcccgtc gctacggaca gattttcgcc cgccaacggc gcgctggccg
    10741 ccctgtttca gtgaacgaca tgtggatcgc cgcgtcggct atcgacagtg gcggccacct
    10801 ggtgaccttc gacaaagact tcggccgcat cgaagcgctt gattgcacaa tcctggctcc
    10861 ctgaaggcgg ggacgatgat caccgcattc tgatctcttg tctctttccc tgatcgctga
    10921 ccttgatccg gccgctgacg caagcgctta ccaaacctaa cccgcgttcc gacgtagaat
    10981 caagggacac gtcgtcccct cggggaaagc aggtctattc atgttcgagt ccgctacacc
    11041 cgccacatcc actccccgct tcaagccctc gtttcccttc aaactgccgc ctctgcgagg
    11101 aggattcgta cgattcctgc tgcgcgccag cgtgatcctc gtcccgatca tcttcatgcg
    11161 ggcgtgcttc attacgtacg ttccgctcga ccagatcggg ctgcgtcaga tctcgttcgg
    11221 catgagcaag gggctgcaga agcagctcgt ggagcccggc taccggcgcg caatttcggg
    11281 ttacgagacc atccgcacgt ttccgcgcaa catccaggcg gtagagttca cgaacgatga
    11341 gagcgagcgc ggcgctgacc accgcacggt cgcggcgatc aacgtgccca ctgtcgacgg
    11401 ctaccccgtc gcggtcgacg tgaccgtcct ctaccgcatc gccgatccct ttctcgtcgt
    11461 gtcgaaattc ggtttcggac gtggttacga agacaacgtc gtcctccgtt ttaccgatcc
    11521 cgccatcaag cagcacctcg gcgaattgcg cgccgaggag ttctaccgcg atcaacgaat
    11581 cgccaaggta cgcgatctga agcgcgagct gtccgagaag ttccgtcaga acggcttgga
    11641 gctggcagac gtgctcatcc ggcaatacga ctaccccgac acgttccagc aactgacgga
    11701 gcagaagaag attcaggatc agtcggtgct gacgaaccgg gcgctggcca agcaggccga
    11761 ggtcgatacg cgattgaagc aaacgactgc cgagggacag aactcgatca acgtgcgcag
    11821 cgcggagttt caggcacaga tcaccgggct caatgcgcaa aaggattttt acgagcgctc
    11881 caaacatgcc gaagcggatc tgctcgtcaa gagtgcggaa gcgcagggca ccgaggaaat
    11941 caacaaggcc atggaaggcg cagggtccga caaactcctg cggctgcgca aagggctggc
    12001 gattctcaat ggcatccagg gtccgattta catcagcgaa gacccgaccg acatcggcaa
    12061 gctgacgggc aaacactgag gcgacaatga aaatacgact gatcgcattg ctcgccatcg
    12121 ccccgctctt cgccgcctgt acgccgcaca cgacgcaggc cacggaggtc ggcgtccgct
    12181 tcaacaagat tacgcaatcg acggaaatct ccgatccggg agcgacctac ttctttgcgc
    12241 cggtcattaa cgactgggcg acgtatgaca cgtcgacgca gaacctcgtc atgtcagcaa
    12301 aggcgactga aggcgaccgc aaagagaagg acgacctccg cttcaagacg cgggacggaa
    12361 acgacatcga aacggatgtc accgtgcgct ggcggatcga tccaacgaaa gctgcataca
    12421 tctggcgcac ggtcgcgccg aacactgtgg cgctgaaaga gcgcctcgtg cggccgctgg
    12481 cacgtgcgta tgtgcgcgac gtgctcaacc ggctcgattc cgaggagtat tacaaccctg
    12541 atctgcgctt cgcggcggcg accgatgcga cgaaggtcat ggcggtacaa ttgaagcctt
    12601 atggcgtcat cgtcgagcag gtcatcctcg gcgacttctc gttcaagccc gactaccaga
    12661 acctgatcaa taagcgcaaa gaggcagaga agcaggccga gaaactggag tcgcagattc
    12721 tcgctaccct ggagtcgaac aaagcaaatt tgcagtcgaa ggtggctgac ctcaccgagc
    12781 agctcacgca ctcacaaggc gatttcgagc aagccaagcg agccgcggac gcgtatgtcg
    12841 tccgcaagca gcaggacgcg caggcaacgc tctcggagaa gagcgccgtg gcgcagggca
    12901 tcaagaaaga gcgcgccgcg ctcaatggca gcgcaggcga cgcgtatgtg agcctgcagt
    12961 tgatcgacgc attgcagaag aaagagattc gccagattcc gaagctgcca aacgggaatg
    13021 tgatcatcga cggaaacaag cttcttgaac agctcggcgt catccaatac caacagaaga
    13081 agaacgacca gtaatgctca atatcgcagg aagcgaatac gactacgcgc aggtcgtgta
    13141 cgtggtgctg caggagtgcg agcaccgccg gcgcgggttt gaggaagagg aattcgatca
    13201 ggacatccgc gcctgcgccg cggaaaagat ggcggagatc aaagccgctt acgacgaatt
    13261 cggcggttcg gccgcctatt gggaagcgct cgacaaagag gtcgaggagg ttgtcattcc
    13321 ccgctatctc gtcgcgtcgc gcgagatgac cgaactggag cgaaccaact tcatgatctg
    13381 gcgcggcggc gaccttgcgg cgcgcttcgt cttcgccctg atcgggctct tcatcggcag
    13441 catcatcatc gcgctgccgt tcatccccat cttcgaggat atgttcgcgc tcgcgctgat
    13501 ggcggctggt tttctttatc ccgatctgaa gcgcttcatg ttcgagcgcc gctatacgaa
    13561 ggtgctgaat cacatcgtca gcgacagcgc cggttatcag gaaaacgccc ggctgcacta
    13621 catgacgtcg caggatattc agaaggcgtt cgagcctggg aaccctcgcc aactgccgcc
    13681 gtgagatcga tggcccctat cgatacgcct ctgcagtaca gcgggatgat ggtgtcctac
    13741 gccggtagcg cggaggatgt catgctccgc cgcttctttg gcgatcaggg ga
//
LOCUS       DATLVW010000324         5699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_12773, whole genome shotgun sequence.
ACCESSION   DATLVW010000324 DATLVW010000000
VERSION     DATLVW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12773"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3100)
                     /locus_tag="VNN25_09985"
     CDS             complement(<1..3100)
                     /locus_tag="VNN25_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXH91898.1"
                     /translation="MLADTQARNSSRMRLPMVLAFVAIAALLGAYWTYVRSRAAYFAA
                     RDLRVLSTFAGQVDNSFDALNGFVRNFAWYSSEPTPTIVRRSDSNIPDLKIADVLPNF
                     QSGQTPCEVPEILSRAGGQASPSQQEKYLDESAPSSESTVRTIALAGGARWLRIAYAG
                     VRVNPTMQQQANPFLLNYASPSNAIGQRELRTACGDIDLSSVINPLILQSFLKAFDGL
                     MIVDAKNGEVIYAARPNRGIEIAPADIGVTNVSVMRERVGWRDYAELDASALSQQTRE
                     IDVDVAGDSYKLFTRPFIFAARDVSGRIGPPEETTRWIVCGLVAQSRFQSEVRTISAS
                     RVAIIVAVLILAICWWPFLRIWLSDEHKALTITDAISVVFSAMIVTSLVTLIFIDAVG
                     YSRLSQTGDDQLREFAASMQEDFSSDILRAARALHAIKPWSERRNWSDWQLAGFGESG
                     RVELLTDPDIAAFPYFSQIAWIDPAGNQIYKASRVRRTPRVSVAERTYFQNALNGRTW
                     HIQEQGRAKTGAQAGYEVTLESVRSLTNGQAQAVMAMPTGNKELPVLAMAFDLIDVMH
                     AVPPAGVRFAIVNEDGDVIFHSEQERIGVERFFSETDDARDLRSAILARRERKVSTPY
                     WGEDHSLYVRPLKGTAWSLVTFRQKGLFRAVNIEMLVITILLLLLSTSIYLVIFLIIV
                     LRMPRYRAPSLWPDKKRAEDYTHLIVAYALAAVAISLCIYGLAPRSLLRVLFVAPAQT
                     VLSTYLLLHHRRRSWVYVFAFTGWMVTSAFYAWFIVAGDIGAHLAVSNAPTAMKMLLL
                     FVLLLESATALGYFIGRESATLQAMLARVRYPLSYRVSGVLLLIATSALPTIGFFKIA
                     SWLELETLVKFAQRDLIGKLETRLEKITEASFATAADFTNSTSKEAPSALRRLRADAL
                     PARFGHMFQTAYCVTFWKDRSCNGCAKGSQPIVPDAMANVFPQYSDESVGMRELYGAE
                     ATDGGWRACRSGRVLTMDQRAAIPRPALTLFLPDGTAPPEQRFVVTSEVPLLFPTLIT
                     PIN"
     gene            complement(3305..3535)
                     /locus_tag="VNN25_09990"
     CDS             complement(3305..3535)
                     /locus_tag="VNN25_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91899.1"
                     /translation="MRQRASVAAIGDSDEFERSRLSSQKAKQQTVFNYRHRHARQVRH
                     PSLMPIAQVDRLNPGWQSAERGELLAALGHSE"
     gene            complement(3563..5461)
                     /locus_tag="VNN25_09995"
     CDS             complement(3563..5461)
                     /locus_tag="VNN25_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91900.1"
                     /translation="MLRHARLLAAAGSSLMLLAALTVWSCYRGNPQTQPPAASAQPAG
                     TTLVRTPILATASCASDGGALDINCPAFPNTTPTAQPQWDYFAWNSFIAANWPAVDPA
                     SNNEQRGFPDVTQRFTSANPSSLLTWETFKEKRELFLYPPVIPPNPPIIPQPWNAAPS
                     YGPVSGQIPLCPSSPAPSAAAVHRGNPLAPRRFFGTGGEILDSLDETVEVASEALESQ
                     GQLCSGVPNPMCGTSSQADCCLVPHLPVGPRVWKGSPVKPNPQPVIYEVKVNYDFFNY
                     VIQNQYYLDAITKGVNGAPGAAANNAIQLPYRTSATAGPQGTGNPNRAVGYNAQTVAT
                     YDGSITPTSNLLPMPVGAVHLKAAWMLLQNEDPSKYHTAEAAYFKTENNQKCMAYGTF
                     GLIGLHIIQRIHQGNGANGNPPGGTFIFATWEHVDNDSAGFTYSDFFSGQPFVGPPKR
                     GFYPSPASALPVKRQFPILPGTVAVNNAVHAAIRNINPNSIWLNYQLIGTQFKAITVP
                     PMPIQGNPNDPTNIGQPLFLANSVIETNVGLQNFQGLPPATAAINHFAAPPGQGHITG
                     NNTLRFNRTTNNMSFNGSGYNMGGCMGCHGVAQSRGFSFSFVLLGGQAGADVDTEKNF
                     TVPPQPGQ"
BASE COUNT         1090 a         1584 c         1863 g         1162 t
ORIGIN      
        1 ggtttatggg cgtgatcaag gtcgggaaca ggagcggaac ttctgaagtc acgacaaacc
       61 gctgctcagg tggcgcggtg ccgtccggga ggaagagtgt gagcgcagga cgcggaatcg
      121 cggcgcgctg gtccatagtc agtactctcc cggaccggca cgccctccag ccaccgtcag
      181 ttgcttcggc cccatacagc tctcgcattc cgactgattc gtccgaatac tgagggaaga
      241 cgttcgccat agcgtccggc acgattggtt gcgacccctt tgcgcaaccg ttgcaagagc
      301 ggtccttcca gaacgtcacg cagtacgccg tttggaacat gtggccgaag cgcgccggca
      361 gcgcgtccgc tcggagccgc cgcagcgccg acggtgcctc cttcgaggtc gagttcgtga
      421 agtcagcagc ggtggcgaat gacgcttcgg ttattttctc aaggcgtgtt tcaagcttgc
      481 cgatgagatc gcgttgcgcg aacttcacca acgtttcgag ctccagccag ctcgcgattt
      541 tgaaaaagcc gatcgttgga agcgccgaag tcgcgatcag caacaggact ccgctcaccc
      601 gatacgagag cggatagcgc acccgcgcca acatggcctg gagcgtcgcc gattcgcggc
      661 caatgaagta accaagcgct gttgccgact ccagcagcag cacgaatagc aggagcatct
      721 tcatcgctgt gggtgcatta gagacggcca aatgcgctcc gatgtcgccg gcgacgatga
      781 accatgcgta gaaggcggac gtgaccatcc aaccagtgaa ggcaaaaaca tagacccagc
      841 ttcgccgccg gtgatgaagg agcagatagg tactaagcac cgtctgcgca ggcgcgacga
      901 aaaggacacg gagcaaggac cgcggcgcaa ggccgtaaat gcatagcgag atggccaccg
      961 cggccagagc gtaggcgacg atcaaatgcg tgtaatcctc ggcccgtttc ttgtctggcc
     1021 agagggaggg ggctcgatac cgtggcatac gcagcacaat gatgagaaaa atgacgagat
     1081 agatcgaggt gctgagtagc agaagcagga tagtgatcac cagcatctcg atgttgacgg
     1141 cgcggaagag gcccttctgc cggaacgtca ccagcgacca cgcggtcccc ttgagcggcc
     1201 ggacatagag actgtggtcc tccccccaat atggggtcga gaccttgcgt tcgcgccgcg
     1261 ccagaatggc gcttcgcaga tcgcgtgcat catcggtctc cgagaaaaac ctctcgacgc
     1321 cgattctttc ctgttcagag tgaaagatca catcgccgtc ctcgttgacg atggcgaacc
     1381 tcacgccggc gggtggtaca gcgtgcatca cgtcaatgag atcgaatgcc atcgccagga
     1441 ccggcagctc cttgttaccg gtgggcatcg ccattacggc ctgcgcttgt ccgttcgtca
     1501 gggagcggac cgactcgagc gtcacttcgt agccggcttg ggccccggtt tttgctcgcc
     1561 cctgctcttg gatatgccac gtccgaccat tcaaagcgtt ttgaaaatac gtgcgttcgg
     1621 cgacagacac tcgcggcgtc cggcgtacac gcgacgcctt atagatttgg ttgccggctg
     1681 gatcgatcca ggcaatctgc gagaagtaag ggaacgcggc gatgtcggga tccgtcagca
     1741 actccactct gccactctcg ccgaagcctg ccaattgcca atcgctccag tttcggcgct
     1801 ccgaccaggg cttgatggcg tggagtgcgc gtgcggcgcg aagaatgtcg cttgagaagt
     1861 cctcctgcat ggaggcagcg aactcgcgga gctgatcgtc gccggtctgc gaaaggcggc
     1921 tgtatccgac tgcgtcgata aagatgagcg ttacgagcga cgtgacgatc atggcgctga
     1981 agacaacgga gatggcgtca gtgatcgtaa gcgccttgtg ttcgtcgctc aaccatatgc
     2041 ggaggaacgg ccaccagcag attgccaaga tgagaaccgc cacgatgatc gcaacccgag
     2101 aggctgaaat cgtacgcacc tcggactgga atcgactctg cgcgacgagg ccgcagacga
     2161 tccagcgtgt cgtctcctca ggtggcccga tacgtcccga tacatcacga gcggcaaaga
     2221 taaagggacg tgtaaagagt ttgtagctgt cgccagcgac gtcaacgtcg atctcccgag
     2281 tctgttgcga cagggcggag gcatctaatt cggcgtagtc ccgccaaccg actcgctcac
     2341 gcatcaccga aacgttcgtg acgccgatgt ccgcaggtgc gatctcgatg ccgcgattcg
     2401 gccgggccgc atagatgact tcgccgtttt ttgcgtcgac gatcatgagg ccgtcgaagg
     2461 ccttcagaaa cgactgcagg attagcgggt taatgactga ggagagatcg atgtcgccgc
     2521 atgcagtgcg aagctcgcgc tgtccgatcg cattggacgg agaagcgtag ttgaggagaa
     2581 agggattggc ctgttgctgc atggtcgggt ttacgcgcac tcccgcgtat gcaatccgga
     2641 gccaccgtgc ccctccggca agggctatcg ttcgcacggt cgattcactc gagggggccg
     2701 actcgtcgag gtacttttcc tgctggctcg gggaggcttg gccaccagca cgcgaaagga
     2761 tctccggcac ttcgcatggc gtctggccgc tctggaagtt cggcagcacg tcggcgattt
     2821 tcaaatccgg aatgttgctg tcgctgcggc ggacaatcgt cggggtcggt tccgaggaat
     2881 accacgcgaa gttacggacg aagccgttta gcgcatcgaa ggaattgtca acctgtcccg
     2941 cgaaggtcga cagcactctg aggtcgcgcg ccgcgaagta ggctgcgcga cttcgaacgt
     3001 aggtccaata ggcgccgagg agagccgcga tggcaacaaa ggctaggacc atcggcagcc
     3061 gcatccgact cgagtttcgc gcctgagtat cagccagcat gttgttagaa gggtcgcgaa
     3121 gccgtgattc gtacctcggc gtgtctacgg cttgtggacg gcctctgaaa tgcctgagca
     3181 cttccgctga cacgacaatg ccagatctct ctcgccgatt gcccacctcg ccatgaatag
     3241 tagacggcca cgatcgcggt ttttccgatg agcgtcggcc gtgactggtt cggttgtcac
     3301 attttcattc ggagtggccg agggcggcca acaattcgcc gcgttccgcc gattgccatc
     3361 cgggattgag gcggtcgacc tgtgcaatgg gcatcagtga tgggtgtcgg acttggcggg
     3421 catggcgatg gcgataattg aagacagtct gctgcttcgc tttctgactg gagagtctgc
     3481 tgcgctcgaa ttcgtccgaa tcaccgatgg cggcaacgct tgcgcgctgc cgcatcaaac
     3541 cggacttctt cacgactgac ccctactgtc ccggttgcgg aggcaccgta aaattcttct
     3601 ccgtatccac atccgcgcct gcctggccgc cgaggaggac gaagctgaag gagaagccgc
     3661 ggctttgggc tacgccgtgg cagcccatgc agccgcccat gttgtagccg gagccgttga
     3721 acgacatgtt gttcgtggtt cgattgaaac ggagcgtgtt gttgccggtg atgtgccctt
     3781 ggccgggcgg tgcggcaaag tggttgatcg cagcggtcgc aggcggcagc ccctggaagt
     3841 tctgtagtcc tacgttcgtc tcgatgaccg agttggccag gaagagtggc tggccgatgt
     3901 tggtcgggtc gtttggattt ccctggattg gcattggcgg aacggtgatc gctttgaact
     3961 gggtcccgat gagctggtaa ttgagccata tcgaattcgg gttgatattg cgaatcgccg
     4021 cgtgtactgc gttattcacg gcgaccgttc ctgggagaat tgggaattgg cgctttacgg
     4081 gcagcgctga tgctggcgat ggatagaagc ctctcttggg cggacctaca aaaggctgtc
     4141 cggagaagaa gtccgagtat gtgaagccgg cgctgtcatt gtcgacgtgt tcccatgttg
     4201 cgaaaatgaa cgtgccgccg ggcgggttgc cgttggctcc atttccttgg tgaatgcgct
     4261 ggatgatgtg gaggccgatg aggccgaagg ttccgtaggc catgcacttc tggttgttct
     4321 cggtcttgaa gtacgcggct tctgcggtgt ggtacttcga tgggtcctca ttctgaagga
     4381 gcatccatgc tgccttcaga tgaactgcgc cgaccggcat tgggagaagg ttgctcgtcg
     4441 gtgtaatcga gccgtcatag gtagcgacgg tctgcgcgtt gtagccgacc gccctgttgg
     4501 ggttgccggt gccttgaggg ccggcagtgg cgctggtccg gtatggaagc tggatggcgt
     4561 tgttggcggc cgcgccgggc gcgccattga cacctttcgt tatcgcatcg aggtagtact
     4621 gattctggat gacgtagttg aagaagtcgt aattgacttt gacctcataa atgaccggct
     4681 gcggattggg tttgaccggg ctgcctttcc atacccgcgg accgacgggg aggtgtggaa
     4741 cgaggcagca gtcggcctga ctcgaggttc cgcacatcgg attggggacg cccgagcaga
     4801 gctgcccctg cgactcgagc gcctcggatg caacttcgac cgtctcgtcg agactgtcga
     4861 gaatctcgcc gcccgtgccg aagaagcgtc ggggagcgag aggattgccg cggtgtaccg
     4921 ccgcggcgga aggagcaggg ctgctcggac agagagggat ctggccggag accggaccat
     4981 agctcggtgc cgcgttccaa ggctggggaa tgattggcgg gtttggcgga atcacgggcg
     5041 gatagaggaa aagctcacgt ttctccttga acgtctccca cgtgagaagt gagctcgggt
     5101 ttgcgctggt gaagcgctgg gtgacatcgg gaaagccgcg ctgctcgttg ttcgaggcgg
     5161 ggtcgacggc cggccagttc gcggcgatga aactgttcca ggcgaagtag tcccattgcg
     5221 gttgggcggt gggcgtggtg ttcgggaacg ccggacagtt gatgtcgagt gcgccgccgt
     5281 cgcttgcgca tgaggctgtt gccaggatgg gcgtgcgaac gagagtagtg ccggccggtt
     5341 gcgccgacgc ggccggcggc tgcgtttgcg ggttgccgcg gtaacagctc catacggtca
     5401 gagcggcaag gagcatcaac gaggatcctg cggcagcgag gagacgggcg tgtcggagca
     5461 tgggcttcct cctgcccgtc attatggtgc tgttcgtcgt tttgatcggg atgttctcgc
     5521 ttctctgttt ctgctcgcga cgtcatcctc tcacgcacgt cctcggcaag ggccgggcgc
     5581 gccgcgatcg gcgcgcccgg cttactgcat gactacatca ctgctcagac gtgggcgttg
     5641 gcgcaggtgc cggcggaact ttcggctgct ggttgcgtcc gaagcggttt gccttccgc
//
LOCUS       DATLVW010000325        14487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_508953, whole genome shotgun sequence.
ACCESSION   DATLVW010000325 DATLVW010000000
VERSION     DATLVW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508953"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VNN25_10000"
     CDS             complement(<1..980)
                     /locus_tag="VNN25_10000"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXH91901.1"
                     /translation="MRILVLGAGRMGFGAVHDLIQQPDVDEVTVADVAADRANHVASV
                     VDGNVTPRAIDVSNHADVVALMRGHDSAISCVNYWLNERLARAAIEAGTNFCDLGGNN
                     DIVDAELALDAEARARGVNIVPDCGLAPGMVAVLVAHGATQFEKLDEIHIRVGGLPQN
                     PKPPLDYQLVFSVEGLINEYIERARVIRDGRITMVDSMTEVEEIEFPEPFGKMEAFQT
                     SGGTSTLPETFLGKVRELDYKTIRYPGHCAKFKTMIDLGLCSSEKMTVDGVQVAPRRV
                     LGDLLVRNLPADEPDVVLVRVEFVGDGKRLRYDIIDRYDEATGLSAMMRTT"
     gene            1056..2045
                     /locus_tag="VNN25_10005"
     CDS             1056..2045
                     /locus_tag="VNN25_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91902.1"
                     /translation="MDADKPADDGKALTDSAEEASAEAVAVDPKPIEEVPVEEPPSDE
                     PPVKEPPVEQPPAEETVVDAPQISDPRPPTPDSHPGTRLVVIKHHALVRLSHWLNVPI
                     LFALIVSGLSIYWASPVFLHKPDPVTHSRDYLADLGIAIARHIPGAKADGFWFYDHFG
                     LGVFNLAGALRWHWLFVYLFLATGFLYILGLLLGGGYRALLPRRRMIADGFAMIRYYA
                     GVVPMKILRRPWEHPHITTKYNALQRGAYASMPIAGALALASGWAMHKPVQFWWLERL
                     FGSYDGARIVHFAVMIYFILFLVPHVVLVLVDGWDTMRSMIVGWSGRIGGSHD"
     gene            2038..2907
                     /locus_tag="VNN25_10010"
     CDS             2038..2907
                     /locus_tag="VNN25_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXH91903.1"
                     /translation="MTEETAEETAGELPERYVDEKKLRRQSRRDFILFGTGAFAALAG
                     LWWIAPLGSKKKVFGEHTADALDTLAARLGATRERSERFQNRALTFDDDIAEALYSPN
                     RTVRTYSKRDITPLRNNYEGRTPSPDYIKEWTLTLTGLASSNDVVLRLPDLSSLAQHE
                     QITRLCCVEGWSAIAGWSGVRFGDLLRAYPPAPGAKWAAIESAVNLDGNGNPDPYYVS
                     IDLPTARHPQTLLATHLNGTPLGADHGAPLRLVAPMKLGLKNIKAITKIAYTVDEPRD
                     FWGERGYSKYDGL"
     gene            3221..3964
                     /locus_tag="VNN25_10015"
     CDS             3221..3964
                     /locus_tag="VNN25_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91904.1"
                     /translation="MDEQRRKKLLRHVKAFRGLVKMVDNLVAERHHSVSDEAFAQVRA
                     GVDDAVKDLSDVIPSFSDAAMQGEASWDEGSKEYKPAPIRGFLQRAIAACEAELEEAI
                     PAEVVGPTLDFSFMHDKRLRLIVERDYPELLVAFTASCKKSCLMLAGSIIESVLLDLA
                     LRDVAMATKTSAAPKSPDPMRWSLDELINVAVELQPTLAPVQAMSHGVRQYRNLVHPA
                     AEIRSSLKVEIEEAKVAITLISILHRELQ"
     gene            complement(4036..4518)
                     /locus_tag="VNN25_10020"
     CDS             complement(4036..4518)
                     /locus_tag="VNN25_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91905.1"
                     /translation="MIEPTIAMVTSASQAKERNLFNVYDGEFALELRSQITPLFQQLA
                     SLAADGLFTIDSKAAASSVQALQSQKWAQHAAKQPGGAELDPYVAVMERSVKKVLNRR
                     KGSPVPTPTPTPTPAATPGELPPGAKGFMAPRPAPAKTAEPENVADLFDKALDDVTKK
                     "
     gene            complement(4445..4717)
                     /locus_tag="VNN25_10025"
     CDS             complement(4445..4717)
                     /locus_tag="VNN25_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91906.1"
                     /translation="MSATDTSPSLSTESTPDGPAAAAAKAVELIAQQYRDAIAGLLTI
                     MPLLKYDSRQTRRGCQREVRCGLDRTDDRYGHIRFAGEGAKPVQRV"
     gene            4957..5214
                     /locus_tag="VNN25_10030"
     CDS             4957..5214
                     /locus_tag="VNN25_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91907.1"
                     /translation="MIRINSKMIRIKNKMIPISPKMIQIQKNDDPDHFEDDPDQKQDD
                     PDLSKDDPDQRKDDPDLSRDDPDLRKDDPDLPKDDPDLRKR"
     gene            complement(5440..5820)
                     /locus_tag="VNN25_10035"
     CDS             complement(5440..5820)
                     /locus_tag="VNN25_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH91908.1"
                     /translation="MNETGARVLVVEDNDMNMQLVEYLLEEGGYQIVKAASGEDALAI
                     ARGGEPVDLILMDIHLPGIDGLSVVHEMKADERTKSIPILALTAHAMRGDRDRFLEAG
                     CDGYISKPIDVKTFLTSVRQYLSA"
     gene            complement(5828..6781)
                     /locus_tag="VNN25_10040"
     CDS             complement(5828..6781)
                     /locus_tag="VNN25_10040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXH91909.1"
                     /translation="MRKDIVIAITGIAVVAAICFGLALMKPDFKPFASFRSATPATTE
                     EGKDHVIMKVNDEGITETEFQAAFSQLPEDMQRQFGSEPGKQAFAEQLVRLKILEQEG
                     RKLGVEKDPKVAGQLAADRTNIIAAAAAQKLLPAPTDAAVQKFYNDNKQKYEATELSH
                     ILIAYQGGAVPPRSGAPLSQQDAMKKTMAIEQQLKQGADFAQLAKQVSDDASSADRGG
                     VLGPFSPGMLPPEIESHIIQLQPGQISDPVPSRYGIHIFKAGARTAQPFEQVKQGIAQ
                     RVQRQSTLDKIELMRKAAKVDFDPKFFPEAQQAKTPAKKPS"
     gene            7465..9066
                     /locus_tag="VNN25_10045"
     CDS             7465..9066
                     /locus_tag="VNN25_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXH91910.1"
                     /translation="MAKITIDGVEVDVQEGINCIEAAKAADIHVPHFCYHPSLSVVGQ
                     CRMCLVEIEGMPKLQAGCSTTVKEGMKIHVWNDKVDEARKGQMEFLLINHPLDCPICD
                     KAGECPLQDYAFNYGSVASRYGEFKRTYPGMDRTAIGPHVVQNMNRCIHCTRCIRFTS
                     EITETGELGFFKRGASTEVGVFPGTPLNNWMSACVTDVCPTGALTTREFRFESRVWNL
                     ESAESVCNGCDVGCNIFIGFRQGQIFRYRPRVNPEVNDHWLCDFGRFSYERYDTDRVV
                     VPKVRNDDEYLGISTWNEALDAIGTAVRGASKTAAIFSANMTNEEAWLAKDVFVERMG
                     ASAGVMVDAIEPIKMKSRTEWIVGTQAAPNYRGVAAALGVGSGEWGVGNTPASDPISH
                     TPPPTPHPLIENLLTEGAQGIDVLFITDANFSERAKDPAVVANLRKAKFLVVMSWDAN
                     HPLNDVADVLIPSTIHAEKEGTFTNLQGRVQRIHQAYPPKGQAVSDLEVYRRIGAMLY
                     PDASELRTLDHIPTLPIADAATVQA"
     gene            9074..10144
                     /gene="nuoH"
                     /locus_tag="VNN25_10050"
     CDS             9074..10144
                     /gene="nuoH"
                     /locus_tag="VNN25_10050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXH91911.1"
                     /translation="MHGIDWVDVGLSVLKIAIVLGAMLNVTPIMVWVERRGSALIQDR
                     PGPNRVGPFGLFQSFADAFKFILKEDMLPAKANKILYTIAPMFGLIPALTTIAVVPWG
                     RPFALPGGRLFEPIIANVNIGILLIFALASMGVYGIVMAGWASANKYSLFGGIRASAQ
                     MISYELALTLAAVSVLLAAGSLSLTDVVWRQTGSFVLFNVIKLPAWNIFSPSMWMAFI
                     VFFVSGFAETNRMPFDFAEADAELVAGYHTEYSSMKFALFFMSEYMAMATMSALVTTL
                     FLGGWDIPWYTEPATWVGFALSVLSFVTKVSIILFIFVWVRWTIPRLKYDLLMKLGWK
                     VFLPMAVVNVVIVALFIARGWL"
     gene            10141..10722
                     /locus_tag="VNN25_10055"
     CDS             10141..10722
                     /locus_tag="VNN25_10055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXH91912.1"
                     /translation="MITFAIFFFFAAIAVIFAATVVLHRNPVVGALSLVASFFALAVM
                     YVLLEAPFLAALQVIVYAGAIMVLFLFVIMLLNLQHQEEPRTRPVQQFLGYTSSAAFG
                     LALIYYLAKFGVTQLPDGPFVSDARTVGIRMFEAYIFPFEMVSILLLAAIVGALLLSG
                     RTGASGVAPLSSGTTTENLAAPHDETPRIEVAP"
     gene            10719..11021
                     /gene="nuoK"
                     /locus_tag="VNN25_10060"
     CDS             10719..11021
                     /gene="nuoK"
                     /locus_tag="VNN25_10060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXH91913.1"
                     /translation="MIPTHWFLILSAILFTIGVCGVLTRKNGITIFMCIELMVNAVNL
                     TFVAYARQLHQLHGLLFVFFVMAIAAAEAAVGLAIFISLFHHRHTIDVDEANLMRW"
     gene            11163..11573
                     /locus_tag="VNN25_10065"
     CDS             11163..11573
                     /locus_tag="VNN25_10065"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HXH91914.1"
                     /translation="MDKTEKLIDWEENERKDRNRDGIDDRIGPPGVDIAAGSQQLSDR
                     LRENNGVDPSLSGGDIDAQWDMAESQGDEASTGSTPTPGQNNTEETAAAMGITYADDE
                     TLKVGEKEEERDKHRWELDPASSEDYRERLKDQG"
     gene            11704..13623
                     /gene="nuoL"
                     /locus_tag="VNN25_10070"
     CDS             11704..13623
                     /gene="nuoL"
                     /locus_tag="VNN25_10070"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXH91915.1"
                     /translation="MLNKLWLIPILPFAGFLLNGLFGKRLGKGFVTTVALLASLGAAI
                     AGTIAVVQYHAAYPTGGRHLNLVYNWIASGSIGADISFQLDPLSVVMLMVVTWVGFLI
                     HLYSVGYMAHEDGYGRYFAYLNLFLSEMLVLILGSSYLLMFVGWEGVGLCSYLLIGFY
                     YETDYAPAAGKKAFVVNRIGDFGFLIAMFLMFAYFGTVDFAKVSSIAAGGGVEHWLLT
                     AICLLLFLGAVGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVVRSNALFR
                     LAPGAMMVVALIGALTALFAATIGIRQWDIKKVLAYSTVSQLGFMFIGVGVGAFTAGI
                     FHLITHAFFKACLFLGSGSVIHAMSGEQDMRKMGGLKSKIPITYWTFAIATYAIAGFP
                     PFAGFFSKDEILASALNNPYFPKPFSWLIWSLGTLAAFCTAYYMYRLVYLTFFGNFRG
                     THEQEHHLHESPASMTIPLIILAALSAVGGALLGWPHHHFLAAWLKPIFPEIGGVPEA
                     QYEISPNVEYALMLVSSAIAVIGWWIARTRFRTRGLDADNQFAQNMPAVARGMENKWY
                     VDEAYDATIVRPLEGFSQFLWRGIDALIDGTLAIVGYIVAAIGDLLRFFQTGNVRNYA
                     LMFFLGVIVFIWVLA"
     gene            13620..>14487
                     /locus_tag="VNN25_10075"
     CDS             13620..>14487
                     /locus_tag="VNN25_10075"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXH91916.1"
                     /translation="MSNILSITLWLPIAAAVILLFFPKTAASAIKGFGLLASLATFVA
                     SLGILNGFQEGVAGLQLTESLAWIPQWGIRYALGVDGISLWLILLTTLLTPVVFLSSW
                     NTIQKHPKEYVIAFLIMEAAMIGVFVASDLLLFYVFFELTLLPMYLVIGVWGGANRIY
                     AAIKFFLFTIAGSLLMLLGIIYLAFAHFHDTGVLTFTITELYGTTLAPHAQVLLFFAF
                     ALAFAIKVPLFPLHTWLPDAHVEAPTGGSIILAGVMLKMGTYGFLRFVLPFFPDATAK
                     YSGLLIALAVIGI"
BASE COUNT         2637 a         4625 c         4291 g         2934 t
ORIGIN      
        1 gtcgtgcgca tcatcgcgga gaggccggtc gcttcgtcgt aacggtcgat gatgtcgtag
       61 cggaggcgct ttccgtcacc tacgaactcg acgcggacca ggacaacgtc tggctcgtcg
      121 gcgggaagat tgcggacgag gaggtcgccg agcacgcggc gcggcgctac ttgcacgccg
      181 tcgaccgtca tcttctcgct gctgcagagg ccgaggtcga tcatggtctt gaacttggcg
      241 cagtggccgg gatagcggat cgtcttgtag tccaactcgc gcaccttgcc gaggaacgtc
      301 tcgggtagcg tcgacgtgcc gccggaggtc tggaacgcct ccatcttccc aaagggttcc
      361 gggaactcga tctcctccac ctcggtcatc gaatcgacca tcgtgatgcg gccgtcccgg
      421 atgacacggg cgcgctcgat gtactcgttg atgagcccct cgacggagaa gacgagctgg
      481 tagtcgagcg gcggcttggg attctgcggc aggccgccga cacggatgtg gatttcgtcg
      541 agcttctcga actgcgtagc gccgtgcgcg acgagcactg ccaccatgcc gggggcaagg
      601 ccgcaatcgg ggacgatatt gacccctcgg gcgcgcgctt ccgcgtcgag ggcgagctcg
      661 gcgtcgacga tgtcgttgtt gccgccgagg tcgcagaaat tggtgccggc ctcgatcgcg
      721 gcacgggcca ggcgctcgtt gagccagtag ttgacgcaac tgatggcgct gtcgtgaccg
      781 cgcatgagtg cgacgacgtc cgcgtgattg ctgacgtcga tggcgcgtgg cgtgacgttg
      841 ccgtcgacga ccgaggcgac atggttggcg cgatcggcgg cgacgtcggc aactgtgact
      901 tcatcgacgt cgggttgctg gatgagatcg tggacggcgc cgaagcccat gcggccggcg
      961 cccagaacga gtatgcgcat gaggaggcgc atggtagttt atctgtgtct ctctgcgtga
     1021 tcggcggacg agtttttcat ccgcagatga cgcagatgga cgcagataaa cccgccgatg
     1081 atgggaaggc gcttactgac agcgccgagg aagcatctgc cgaggcagta gcggtcgacc
     1141 cgaagccgat cgaggaagtg ccggtcgagg agccgccgtc cgacgagccg ccggtcaagg
     1201 aaccgcctgt cgaacaaccg ccggccgagg aaacggtggt cgatgctcct cagatctccg
     1261 acccccgacc cccgactccc gactcccacc ccggcacccg cctcgtcgtc atcaagcacc
     1321 acgccctcgt gcggctgtcg cattggctga acgtgccgat cctcttcgcg ctcatcgtca
     1381 gcggtctgtc gatctactgg gcctcgccgg tttttctgca caagcccgat ccggtcacac
     1441 acagccgcga ctatctcgcc gacctcggca tcgccatcgc ccgccacatt cccggcgcca
     1501 aggctgacgg attctggttc tacgatcact tcggcctcgg cgtcttcaat ctggccggag
     1561 cgctgcgctg gcactggctg ttcgtttacc tcttcctcgc caccggcttc ctctacatcc
     1621 tcggcctgct cctcggcggc ggctaccgcg ccctgctgcc gcggcgcagg atgatcgccg
     1681 acggattcgc gatgatccgc tactacgccg gcgtcgtccc gatgaagatc ctgcggcgcc
     1741 cgtgggagca tccccacatc acgacgaagt acaacgccct gcagcgcggc gcgtatgcgt
     1801 cgatgccgat cgcgggcgcg ctcgccctcg ccagcggctg ggccatgcac aagccggtgc
     1861 agttctggtg gctggagcgg ctcttcggct cgtacgacgg cgcacgcatc gttcacttcg
     1921 ccgtgatgat ctatttcatc ctctttctcg tcccgcatgt cgtgctcgtg ctggtcgatg
     1981 gatgggacac gatgcgctcg atgatcgtgg gatggtcggg ccgcatcgga ggaagccatg
     2041 actgaagaaa cggctgaaga aacggcgggc gaactgcctg agcgctatgt cgatgagaag
     2101 aagttgcgcc gtcagtcgcg ccgcgatttc atcctcttcg gcaccggcgc gttcgcggcg
     2161 ctggcaggac tctggtggat cgcgccgctg ggatcgaaaa agaaggtctt cggcgagcat
     2221 accgccgacg cgctcgacac gcttgccgcc cgtctcggcg cgacgcgcga gcgcagcgag
     2281 cgttttcaga atcgcgcgct gacgttcgat gacgacatcg ccgaggcgtt gtattcaccg
     2341 aatcggacgg tgcggacgta ttcgaaacgc gacatcaccc cgctgcggaa caattacgaa
     2401 ggacgtaccc cgtcgcccga ctacatcaag gagtggacgc tgacattgac cgggctggct
     2461 tccagcaacg acgtcgtcct gcgccttccc gatctctcgt ccctcgcgca gcacgaacag
     2521 atcacgcggc tctgctgcgt cgaaggatgg agcgccatcg ccggctggag cggagtccgt
     2581 ttcggcgatc tgctgcgcgc ctacccgccg gctcctggcg cgaagtgggc ggccatcgaa
     2641 tcggcggtga atctcgacgg caacggcaat cctgatccct actatgtctc gatcgatctg
     2701 ccgaccgcgc ggcatcctca aactctgctg gccacccacc tgaacggcac tcccctcggc
     2761 gccgatcacg gcgcgccact ccgcctcgtc gccccgatga aactcggcct caagaacatc
     2821 aaagcgatca cgaagatcgc ctacaccgtc gacgaaccgc gcgatttctg gggcgagcgc
     2881 ggatattcga agtacgacgg gttgtagctg cgccggcctg agtccggcgc gttgtcggtt
     2941 gtcggttggt tgcgcttcgc gcgttgtccc ccctgagagc gcgggcatcc cgccttgcat
     3001 cccgcccgcc gttcccggga tgaagtcgca cctctccgca cgcttgctac gaacccgagt
     3061 gaggcgaaaa ataagcgatc tggccgccgt cgattaactc tctaacaaag cgagtcagcg
     3121 ggtgttcaca cgatttcctc atcagttcgg aacgagtgca tcggctctct ttctgcacga
     3181 ttgaggatga aagactctcg ctgctacgtt tcgcgtcggg atggacgaac agcgccgaaa
     3241 aaaactgttg cggcacgtaa aggcatttcg cggccttgtg aagatggtgg acaatctggt
     3301 tgcggagaga caccactcgg tgtctgacga ggcttttgcg caggtccgag ctggagtgga
     3361 tgacgctgtg aaagatctgt cggatgtcat cccctctttc agcgacgccg caatgcaggg
     3421 cgaggcgagc tgggacgaag gcagcaagga atacaaacct gctccaattc gcggtttctt
     3481 gcaaagagca atcgctgcgt gcgaggcaga actcgaagag gccattccag cagaggtcgt
     3541 cgggccgact ctagacttct cgttcatgca tgacaagcga ttgcgcctga tcgtcgaacg
     3601 tgactatccc gaattactcg ttgccttcac ggccagctgc aagaagtcat gtctcatgct
     3661 cgcgggcagc atcatcgaat ccgttcttct tgacctcgcc cttcgggacg tagcaatggc
     3721 gacgaagact tcggcagctc ccaagagccc agatccgatg cgctggtcac tggatgagct
     3781 aatcaatgta gctgtcgagc tacagcccac gctggccccc gttcaagcga tgtcacatgg
     3841 tgtacgacaa taccggaacc tcgtccatcc tgctgcggag atcaggagca gtctcaaagt
     3901 ggaaattgag gaggcaaaag tcgcgatcac tctaatatcc attcttcatc gcgaactcca
     3961 ataaccgagc ggcgaagggg tcacccgcaa accgccgccc cccacccggc cagtgggggg
     4021 cggcgtgtac ggggactact tcttggttac gtcgtcgagg gctttgtcga agaggtcggc
     4081 gacgttttcc ggctcggcgg ttttggccgg ggccggacgt ggcgccatga aacccttcgc
     4141 gccgggcggc agttcgcccg gggtcgcggc gggggtcggg gttggcgtcg gggtgggaac
     4201 cggggagccc ttgcgacgat tgagaacctt cttcacagag cgctccatca cggcgacgta
     4261 ggggtcaagc tccgcacccc cgggttgttt ggcggcgtgc tgcgcccact tctgcgactg
     4321 cagcgcttgc accgaggagg cggccgcttt gctgtcgatc gtgaacagcc cgtcggctgc
     4381 aagcgaggcc agttgttgaa acagcggggt gatctgactg cgcaactcca acgcaaactc
     4441 gccgtcatac acgttgaaca ggtttcgctc cttcgcctgc gaagcggatg tgaccatagc
     4501 gatcgtcggt tcgatcaagg ccgcagcgaa cttcgcgctg gcagccacgc ctcgtctggc
     4561 gggagtcgta cttcagaaga ggcatgatcg tcagcagccc cgcgatggca tcgcgatact
     4621 gctgggcgat tagctccact gccttcgcgg ccgccgcggc ggggccgtca ggggtggatt
     4681 cggtggatag agagggggaa gtatccgtcg cagacatgaa tcatcaagct ccttcgtcta
     4741 acgtcattga tcaacagccg gccggcctgt cttctcaccc ggcaacgaac gcgttgccgg
     4801 acggtctgat ctctctcaga catacttttg acgtaagagc cctaacgaag gttccatcgt
     4861 aggaagaaat atcttctgcg gagggtgaat ggaggggttc ggatcaaacc agagttgatc
     4921 cggatcaaac cagggttgat cgggaggaga aaaaagatga tccggatcaa ttcaaagatg
     4981 atccggatca aaaacaagat gatcccgatc tctccaaaga tgatccagat tcaaaaaaat
     5041 gatgatcccg atcacttcga agatgatccg gatcaaaaac aagatgatcc ggatctctcc
     5101 aaagatgatc cggaccaaag aaaagatgat cccgatctct ccagagatga tccggatcta
     5161 agaaaagatg atcccgatct ccccaaagat gatccggatc taagaaaaag atgatccgga
     5221 tcaattcaaa gatgatccgg atcaaagaag gaatgatcgg aatcggccgt tccgggatgc
     5281 agaggatcag gcctttgctt tcttgtcagt tgcaactcat tgatagccag ttacttgcgc
     5341 gctgaatggc cggtttggaa tcaaccacag agacacagag gacacagagt cgaaaccctt
     5401 gctctgcgac ctctgcgtct ctgtggtgtg agccgtttgc tacgcgctca ggtactgccg
     5461 caccgaggta aggaacgtct tcacgtcgat cggtttggag atgtagccgt cgcagccggc
     5521 ttcgaggaag cggtcgcggt cgccgcgcat ggcgtgggcg gtcagggcca ggatagggat
     5581 cgatttcgtg cgctcgtcgg ccttcatttc gtggacgacg ctgaggccgt cgatccccgg
     5641 caagtggatg tccatcagga tcaggtcgac cggttcgccg ccgcgcgcga tcgccaacgc
     5701 gtcctcgcca ctggcggctt tgacgatctg gtagccaccc tcttcgagca gatactcgac
     5761 gagctgcatg ttcatgtcgt tatcctcgac gacgaggacg cgggctccgg tctcgttcat
     5821 cggcggtcta ggacggcttc tttgccggcg tcttcgcctg ctgcgcttcg gggaagaact
     5881 tcggatcgaa atcgaccttg gccgctttgc gcatcaattc gatcttgtcc agcgtgctct
     5941 ggcgctgcac gcgctgggca atcccctgct tcacctgttc gaacggctgg gcggtgcggg
     6001 cgcccgcttt gaagatgtga atgccgtagc gcgacggcac gggatcgctg atctggccgg
     6061 gctgaagctg aatgatgtgc gactcgatct cgggggggag catgcccggg ctgaaggggc
     6121 cgaggacacc gccacgatcg gcgctgctgg cgtcgtcgga cacctgtttg gccaattgcg
     6181 cgaaatcggc gccctgcttg agctgctgct cgatggccat cgtcttcttc atcgcgtcct
     6241 gctggctcag cggagcgccg gagcggggcg gtaccgcccc cccctgataa gcgatgagga
     6301 tgtggctcag ctccgtcgcc tcgtatttct gcttgttgtc gttatagaac ttctgcacgg
     6361 cggcgtcggt cggtgccgga agcagtttct gggcagcggc ggcggcgatg atgttggtgc
     6421 ggtcggcggc gagctggccg gcaaccttgg ggtccttttc gacaccgagc ttgcgccctt
     6481 cctgctccag gatcttcagc cggacgagct gctcggcgaa ggcctgcttg ccgggctcgg
     6541 atccgaactg gcgctgcatg tcttccggaa gctgcgagaa ggcagcctgg aactcggtct
     6601 cggtgatccc ctcgtcattc actttcatga tgacgtggtc cttgccctcc tcggtcgttg
     6661 ccggagtggc actgcggaat gacgcgaacg gcttgaagtc gggcttcatg agagcaagcc
     6721 cgaagcagat ggcggcgacg actgcgatcc ctgtgatcgc gatgacgatg tcttttctca
     6781 aggtatctcc gtggattgcg cggccctggg ggccgcgcgt tatcggttgt cggttgtcgg
     6841 tggttccggg cgggtttggc cgcttgttga tcggagtcta caacgtactc gaacccgtcg
     6901 gcagccccga caacgcggcg cgcggtgacg caaccaacct gacgaccggc gcgttgtcag
     6961 tagtagtagc cgataagcgg ttcgctagtg atccgttgcc ggtggtccgt tccggggcgg
     7021 ctagccctcg gcttgcccac ttttctcaca cttctttcgc aactctttcc aatcgagcgc
     7081 cttctccccc acgcttcttg tggggagaag gcgctcgatg ggaaagcact ccaaagaata
     7141 aggaagaggt gtagcaagag cgtgcatcaa gcgcgtgaga aatgacggct ggaccgcgtg
     7201 ttcatcaggg tctacaacgc cccgaaccct tccgcagccc cgataacgcg gcgcgcagcg
     7261 ccgcaaccaa ccgacaaccg acaacgcggt ggccgccagg ccaccgcaat cattcggcgc
     7321 cgcaatgatt gacgccaact accccagcaa gtattctccg cgcttgtgaa aactttcacg
     7381 gattctcatc cggaaggcct tcacgaacag ccagctccac ggccctgatc ctgcatccct
     7441 tcggagcccc accgagaaca acgtatggca aaaatcacca tcgacggcgt cgaagtcgac
     7501 gtgcaggaag gcatcaactg tatcgaagcg gccaaagccg ccgacatcca cgttccccat
     7561 ttctgctacc acccctctct cagcgtcgtc ggccagtgcc gcatgtgcct cgtcgagatc
     7621 gaaggcatgc ccaaactgca ggcaggttgc tcgaccacgg ttaaagaggg gatgaagatc
     7681 cacgtctgga acgacaaagt cgacgaggcg cggaaggggc agatggagtt tctcctcatc
     7741 aaccatccgc tcgattgccc gatctgcgac aaagccggcg aatgcccgct gcaggactac
     7801 gccttcaact acggctcggt cgcttcccgc tatggcgagt tcaaacgtac ctatcccggc
     7861 atggaccgca ctgcgatcgg cccgcacgtc gtgcagaaca tgaaccgctg catccactgc
     7921 acgcgctgca tccgcttcac ctccgagatc acggagaccg gcgagctggg attcttcaag
     7981 cgcggcgcat cgaccgaggt gggcgtcttt cccgggacgc cactgaacaa ctggatgtcg
     8041 gcgtgcgtta ccgacgtctg cccgaccggc gcgctgacca cacgcgagtt tcgcttcgaa
     8101 tcgcgcgtct ggaacctcga gtccgcggaa tccgtctgca acggctgcga cgtcggatgc
     8161 aacatcttca tcggcttccg gcagggacag atcttccgtt accggccgcg tgtgaatccc
     8221 gaggtgaatg atcactggct gtgcgacttc ggacggttct cgtatgagcg ctacgacacc
     8281 gaccgcgtgg tcgtccccaa agtccgcaac gatgacgagt acctcggcat ctcaacgtgg
     8341 aacgaagcgt tagacgcgat cggcaccgcc gtacgcggcg catcgaagac cgccgcgatc
     8401 ttctccgcaa acatgacgaa cgaagaggcg tggctcgcca aggatgtctt tgtcgagcgc
     8461 atgggcgcat cagccggcgt gatggtcgac gcgatcgagc cgatcaagat gaagtcacgg
     8521 acggagtgga tcgtagggac gcaggccgcg ccgaattacc ggggcgtggc agcggcgttg
     8581 ggagtgggga gtggggagtg gggagtggga aacactccgg cctcagaccc aatctcccac
     8641 accccacccc ccacacccca ccccctcatc gaaaatctct taaccgaggg tgcacaaggc
     8701 atcgacgttc tcttcatcac cgacgccaat ttcagcgagc gcgcaaaaga ccctgccgtc
     8761 gtcgccaatc tccgcaaagc gaagttcctc gtcgtcatgt cgtgggacgc caatcatccg
     8821 ctgaacgacg tcgccgacgt cctcatcccc tcgacgatcc atgccgagaa ggaagggacc
     8881 ttcaccaacc ttcaggggcg cgtgcagcgc attcatcagg cctatccgcc gaaagggcag
     8941 gccgtgagcg acctcgaggt ttatcgccgc atcggggcga tgctctatcc cgatgcgtcc
     9001 gagctgcgca cgctcgatca cattcccaca cttccgatcg ccgacgcggc gacggtgcag
     9061 gcgtaggacg acgatgcacg ggatcgactg ggttgacgtt ggcctctcgg tactgaagat
     9121 cgcgatcgtc ctcggcgcga tgctgaacgt cacgccgatc atggtgtggg tggagcggcg
     9181 cggcagtgcg ctcatccagg accgtcccgg cccgaaccgc gtcggtccgt tcggcctttt
     9241 ccagtccttc gccgacgcct tcaagttcat tctcaaagag gacatgctgc cggcgaaggc
     9301 aaacaagatc ctctacacca tcgcgccgat gttcggactg attccggcgc tgacgacgat
     9361 tgccgtcgtg ccgtggggca ggccgttcgc gcttccgggc ggacgcctct tcgagccgat
     9421 catcgccaac gtcaacatcg gcatcctgct gatcttcgcg ctcgcctcga tgggcgttta
     9481 cggcatcgtc atggccggct gggcctcggc caacaaatac tccctcttcg gcggcatccg
     9541 cgcctcggcg cagatgatct cgtacgagct ggccctcacg ctggccgcgg tcagcgtgct
     9601 cctcgccgct ggatcgcttt cgcttacgga cgtcgtctgg cgccagaccg gatcgttcgt
     9661 tctcttcaac gtgatcaaac tcccggcctg gaacatcttc tcgccgtcga tgtggatggc
     9721 tttcatcgtc ttcttcgtct caggctttgc cgaaacgaac cggatgccgt tcgactttgc
     9781 ggaagccgac gccgagctcg tagccggcta tcacaccgag tattcgtcga tgaagttcgc
     9841 gctcttcttc atgtccgagt acatggctat ggccacgatg tcggcgctgg tgacgacgct
     9901 cttcctcggc ggctgggaca tcccctggta caccgagccg gccacctggg ttgggttcgc
     9961 gctgtcggtg ctgtcgttcg tgaccaaggt cagcatcatc ctcttcatct tcgtctgggt
    10021 acgctggacg atcccacgcc tcaagtacga cctgctcatg aaactcggat ggaaagtgtt
    10081 cctgccgatg gctgtggtga atgtcgtgat cgtggcgctc ttcatcgccc gaggatggct
    10141 atgatcacgt tcgcgatctt tttcttcttc gccgccatcg ccgtgatctt cgcggcgacc
    10201 gtcgttctgc atcggaatcc ggtagtcggt gcgctttcgc tcgtagcgtc gtttttcgcg
    10261 ctggccgtga tgtacgtgct gctcgaagcg ccgttcctgg ccgcattgca ggtgattgtc
    10321 tacgccggcg cgatcatggt cctcttcctt ttcgtgatca tgctgctcaa cctgcaacat
    10381 caggaagagc cgcgcacacg tccagtgcag cagtttctcg gctacacgag cagcgcggcc
    10441 ttcggtctcg cactcatcta ctacctcgcc aagttcggcg tcacgcagct tcccgatggc
    10501 cccttcgtgt cggacgcgcg gaccgtcggc atccggatgt tcgaggccta catcttcccg
    10561 ttcgagatgg tctcgatcct cctgctggct gcgatcgtcg gcgcgttgct cctgagtggc
    10621 cgcaccgggg caagcggcgt ggcgccattg tcctcaggaa caacgactga aaacctggcc
    10681 gcgccgcacg acgaaacgcc acgtatcgag gtggctccat gatcccgacc cactggttcc
    10741 tcatcctgag cgcgattctc ttcacgatcg gcgtctgcgg cgtgctgacg cgcaagaatg
    10801 gcatcaccat cttcatgtgc atcgagctga tggtcaacgc agtgaacctc accttcgtcg
    10861 cctacgcccg ccagctccac cagcttcacg ggctgctctt cgtctttttc gtcatggcca
    10921 tcgccgccgc cgaagccgcc gtcggcctgg ccattttcat ttcgttgttc catcaccgcc
    10981 atacgatcga tgtggatgag gcaaatttga tgaggtggta atgggggggt ggggagtagg
    11041 gagtgggggg tgggagaaac ggttcgaaat tgacgttcga gctgcttttc tcccacaccc
    11101 cacccccgac tccccactcc ccggcacgaa cgtcgcattt tcgagctcgc aggagcgcga
    11161 acatggacaa gaccgaaaag ctcattgact gggaagagaa cgaacgcaaa gacaggaatc
    11221 gggacggcat cgacgaccgt atcggaccgc ccggagtaga catcgccgcc ggatcgcagc
    11281 agctttccga ccggcttcgc gagaacaacg gcgtcgatcc ctcgctctcg ggcggtgaca
    11341 tcgacgcgca gtgggacatg gccgaatcgc agggcgacga ggcttcgaca ggaagtacac
    11401 cgacacccgg ccagaacaac acggaggaaa ccgcagcggc gatggggatc acctacgccg
    11461 acgacgagac gctgaaagtc ggagagaaag aagaggaacg cgacaaacat cgctgggagc
    11521 tcgatcccgc ctcttccgaa gactaccggg aacgactgaa agatcaaggc tgatgcgggg
    11581 ctctcctcgc accggcgtat gcgaggagaa acgtgaggaa gcagagatca gagactagag
    11641 actaggatcc caagaaaaga cctctgatct cttgtctctg atctctgctt cccacacccc
    11701 gaaatgctca acaaactctg gctcatcccc atcctgccgt tcgccggctt tctgctgaac
    11761 ggcttgttcg gcaagcgcct cggcaagggg tttgtcacta ccgtggcgct cctggcatcc
    11821 ctcggcgccg caatcgccgg aaccatcgcc gtcgtgcagt atcacgccgc ctatcccacc
    11881 ggcggccgtc acctcaatct cgtctacaac tggattgcgt cgggatcgat cggcgccgac
    11941 atctccttcc agctcgatcc cctcagcgtc gtcatgctga tggtcgtcac ctgggtcggc
    12001 ttcctcatcc atctctactc cgtcggctac atggcgcacg aggatggcta cggacgctac
    12061 ttcgcgtacc tgaacctctt cctctccgag atgctcgtgc tgatcctcgg cagcagctac
    12121 ctcctcatgt tcgtcggatg ggaaggcgtc ggcctctgct cctatctgct gatcggcttc
    12181 tactacgaga ccgattacgc ccccgcggcg ggcaagaaag ccttcgtcgt caaccgcatc
    12241 ggcgacttcg gcttcctcat cgccatgttc ctgatgttcg cgtatttcgg gactgtcgat
    12301 ttcgcgaagg tgtcgtcgat cgcggccggc ggcggtgtcg agcactggct gctgaccgcc
    12361 atctgtctcc tccttttcct cggcgcggtc ggcaagagcg cgcagattcc gctctacgtc
    12421 tggctgcccg acgccatggc cggcccgacg cccgtctccg ccctcatcca tgccgccacg
    12481 atggtcaccg ccggcgtcta catggtcgtc cgttcgaacg cgctcttccg gctcgcgccc
    12541 ggcgcgatga tggtcgtcgc gctgatcggc gcgctgaccg cgcttttcgc cgcaacaatc
    12601 ggcatcagac agtgggacat caagaaagtc ctcgcctact caaccgtctc gcagctcggc
    12661 ttcatgttca tcggcgtcgg cgtcggcgcc ttcacggcag gcatcttcca cctcatcacg
    12721 cacgccttct tcaaagcctg tctcttcctc ggatccggct cggtcattca cgccatgagc
    12781 ggcgagcagg acatgcgcaa gatgggcggc ctgaaatcga agatccccat cacctactgg
    12841 accttcgcca tcgccacgta cgcgatcgcc ggcttcccgc cattcgccgg cttcttctcc
    12901 aaagacgaga tcctcgcgtc agcgctcaac aatccctact tccccaaacc attcagttgg
    12961 ttgatctgga gtctaggcac cctcgccgcg ttctgcaccg cgtattacat gtaccgcctc
    13021 gtctacctga cctttttcgg caacttccgc gggacgcacg agcaggagca tcacctgcat
    13081 gaatcgccgg cgtcgatgac gattccgctg atcatcctgg ccgcactctc cgccgttggc
    13141 ggtgcactgc tgggatggcc gcatcatcac ttccttgcgg cgtggctcaa gccgatcttc
    13201 cccgagatcg gtggcgtccc cgaagcacag tacgagatct cgccgaacgt cgaatacgcg
    13261 ctgatgctcg tctcgagcgc catcgccgtc atcggctggt ggatcgcccg gacgcgcttc
    13321 cgcacgcgcg gcctcgacgc cgacaaccag ttcgcgcaga acatgcctgc ggtcgctcgc
    13381 gggatggaga acaagtggta tgtcgatgaa gcctacgacg cgacgatcgt ccggccgctc
    13441 gaaggcttct cgcaattcct ctggcgcggc atcgacgccc tcatcgacgg cacgctggcc
    13501 atcgtcggct acatcgtcgc cgcaatcggc gacctcctcc gcttcttcca gaccggcaac
    13561 gtccgcaact acgcgctgat gttctttctc ggcgtgatcg tcttcatctg ggtgctggca
    13621 tgagcaacat cctctccatc accctctggc ttccgatcgc cgccgcggtg atcctgctct
    13681 tcttcccgaa gaccgcggcc tcggcgatca agggctttgg cctgctggcg tcgctggcca
    13741 cgttcgtcgc gtccctcggc atcctcaacg gcttccagga aggcgtggct ggtctgcagc
    13801 tcactgaatc gctcgcctgg attccgcaat gggggatccg ttacgcgctc ggcgtcgacg
    13861 gcatctcgct ctggctcatc ctgctgacta cgctcctcac gccggtcgtc ttcctttcat
    13921 cgtggaacac gatccagaaa catccgaagg agtacgtcat cgcgtttctg atcatggaag
    13981 cggcgatgat cggcgtcttc gtcgccagcg accttcttct cttctacgtc ttctttgagc
    14041 tcacgctgct accgatgtac ctcgtcatcg gcgtgtgggg cggcgcgaac cggatctacg
    14101 cggcgatcaa gttctttctc ttcaccatcg ccggttcgct gctgatgttg ctcggcatca
    14161 tctatctcgc cttcgcccac ttccacgaca ccggcgtcct gaccttcacc atcacggagc
    14221 tgtacggcac gacgctcgcg ccgcacgcgc aggtgctgct cttcttcgcc ttcgcgctcg
    14281 ccttcgcgat caaggtgccg ctcttcccgc tgcacacttg gctgcctgac gcgcacgtcg
    14341 aagcgcccac cggcggatcg atcatcctcg ccggcgtcat gctgaagatg ggtacttacg
    14401 gcttcctccg cttcgtgctc ccgtttttcc ccgatgccac cgctaagtac tctggcctgc
    14461 tcatcgcgct ggccgtgatc ggcatcg
//
LOCUS       DATLVW010000326         5421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_465542, whole genome shotgun sequence.
ACCESSION   DATLVW010000326 DATLVW010000000
VERSION     DATLVW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465542"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VNN25_10080"
     CDS             <1..1020
                     /locus_tag="VNN25_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXH91917.1"
                     /translation="PVDDDDQADVAFVVRSVESLLPDAPAGALVLVSSQLPVGSTRDL
                     EQRAAGREITFGYSPENLRLGRAIEAFTKPDRVVAGVRNERDRVRVTSLLAPFTDRIE
                     WMSVESAEMTKHALNAFLATSVAFINEIASICEQVGADAKEVERGLKSEHRIGPKAYL
                     GPGGAFAGGTLARDVVFLATLGRQHDVPTNLISGVKASNDAHRGWAARRLRDLIGDLA
                     GIRIAVWGLTYKPGTDTLRRSSAIELCRWLAAQGAFVAAHDPAVKSIDIDGVELCVEP
                     LAAAASASALVVMTEWPDYRNIDGGAIENALAGKKIILDANRFLGTQLADQPRVIYAA
                     VGKAV"
     gene            1024..1899
                     /locus_tag="VNN25_10085"
     CDS             1024..1899
                     /locus_tag="VNN25_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH91918.1"
                     /translation="MPYDLTGRTAVITGASQGLGLAIARAYVSAGASVLICARDASTI
                     AAAREELESLRTGATRVFGEAADVADESDVARVVERAISDFGTIDILVNNAGIYGPLG
                     AIDEIDWPAWVRAIEINLLGSVLFCRAVVPHMKRQGKGKIIQLSGGGATNPLPRISAY
                     AASKAAIVRFAETLAEELREHRIDVNSIAPGALNTRLLDEVLAAGPERVGASFYERSL
                     KQKENGGAGLDAGAELALFLASAVSDGITGKLLSAVWDPYPELPQHLDDVTNTDIYTL
                     RRIVPKDRGLKWGVD"
     gene            1884..2870
                     /locus_tag="VNN25_10090"
     CDS             1884..2870
                     /locus_tag="VNN25_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXH91919.1"
                     /translation="MGRRLNVAIVGCGLIGQKRAKALGAATLVACCDAAPDRAAALAR
                     ACGATSQSDWRDVIGREDVDIVIVATTNESLAPIALAAIEAGKHVLVEKPGARSVGEI
                     EPLITAAKARDVRVRVGFNHRYHPALQKARELINDGACGDLMFVRGRYGHGGRIGYDK
                     EWRADPVLAGGGELIDQGVHVIDLARWFLGDFTRVEGYAGTYFWQMPVDDNAFLLLRT
                     EKNLAAFLHVSCTEWKNLFSFEIYGRTGKLAIDGLGGSYGVERLTCYRMLPEMGPPET
                     TSWEYPRGDDSWGLEFREFLDDIQKKRTPSAGLADARAALDVVRKVYEASTK"
     gene            2867..3847
                     /locus_tag="VNN25_10095"
     CDS             2867..3847
                     /locus_tag="VNN25_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HXH91920.1"
                     /translation="MIIARSPLRITLGGGGTDLPSYYREHGGYLLAAAIDKYVYVTVT
                     RPFTPGIFLKYSQLEHVDDVHDVQHPIIREAIRMLNFKTPQIEITTLADIPSGTGLGS
                     SGSFTTALLKALYAHRRKTILPHDLARLACEIEIDRLQEPVGKQDQYIAAHGGITCFR
                     FNPDESVSASPLPISFDTMADLEDNVLLFFTGFSRSAGSILKDQKVRSQAGDDEMIRN
                     LDYVKDLAVRSEAALVNGKVADFGVLMHEHWEHKKRRSGGMSNPKIDEWYELGIQNGA
                     VGGKLVGAGGGGFLMFYAEDRNRLRHAMSRAGLEEVRFRFDFEGTKILLS"
     gene            3914..4531
                     /locus_tag="VNN25_10100"
     CDS             3914..4531
                     /locus_tag="VNN25_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXH91921.1"
                     /translation="MSYTKQHLAEASEVIARLDADAIERMAFMLADLRERAGRLFFLG
                     VGGSAGNCSHAVNDFRKIVGIESYAPTDNVSELTARTNDEGWETVFVEWLKTSRLSSN
                     DMLFIFSVGGGNLEKNISPNLVRALQYAQTVGAKVTGVVGRDGGYTAKVADVCVIVPT
                     VNPENVTPHSEAFQAVIWHLLVSHPALKARQTKWESTSLETSRVS"
     gene            4521..5051
                     /locus_tag="VNN25_10105"
     CDS             4521..5051
                     /locus_tag="VNN25_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXH91922.1"
                     /translation="MFLDRDGVINRAILRDGKPHPPQTLREVELVPDASSSLQRLHEE
                     GFLLIVVTNQPDVARGTQERSTVEAINNHLATELPIDEFVVCYHDDDAACECRKPNPG
                     GILDAAARLGIDLSSSFLIGDRWRDIEAGQRAGCSNYFIDYGYAERQPAPPFSRVHSL
                     AEAVRGIVTRIATEES"
     gene            5048..>5421
                     /locus_tag="VNN25_10110"
     CDS             5048..>5421
                     /locus_tag="VNN25_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HXH91923.1"
                     /translation="MKTLQDLKVKLFADGADQAGMLEMYRKPFVKGFTTNPTLMRKAG
                     ITDYRGFAHEILAAISDRPISFEVFSDEFDEMERQAREIATWGANVYVKIPITNTRRE
                     PAYELVRSLSHDGVKVNVTAMMT"
BASE COUNT         1047 a         1614 c         1708 g         1052 t
ORIGIN      
        1 ccggtggatg acgacgacca ggcggacgtc gccttcgtcg ttcgatccgt cgaatcgctt
       61 ctgcctgacg ctccggccgg cgcactcgtt ctggtttcct cgcaactccc cgtcggatca
      121 acgcgtgatc tcgagcaaag agcggcgggg cgcgagatca cgttcggcta ctcgcctgag
      181 aacctccggc tcgggagagc gatcgaagcg ttcacgaaac ctgatcgcgt cgtcgcgggc
      241 gttcgcaacg agcgggaccg ggtgcgcgtg acctcgcttc tggcgccatt tacggaccgc
      301 atcgagtgga tgagcgtcga atcggcagag atgacaaagc acgcactcaa cgcctttctg
      361 gcgacgtccg ttgcgttcat caacgagatc gcgtcgatct gcgagcaggt tggcgcggat
      421 gccaaagaag tcgagcgtgg tttgaaaagc gagcatcgca taggaccgaa ggcctatctc
      481 gggccgggtg gtgcgttcgc agggggaacg ctggcgcgcg acgtagtctt tctcgctact
      541 ctcggacgtc agcatgacgt gccgacgaat ctgatcagcg gcgtgaaagc gagcaacgac
      601 gcgcaccgcg ggtgggctgc acgccggtta cgcgatctga tcggcgatct ggccggcatt
      661 cggatcgcgg tttggggact cacgtacaag ccgggcacgg acactctgcg ccgttcttcc
      721 gcgatcgagc tgtgtcgctg gctggccgcg cagggagcgt ttgttgcggc gcatgacccc
      781 gcggtgaagt cgatcgacat tgatggcgtc gagctctgcg tggagccgtt ggctgctgcc
      841 gcgtctgcgt ctgccctcgt cgtcatgacg gagtggccgg attaccggaa tatcgatggc
      901 ggcgcgatcg aaaatgcgct cgccggaaag aaaatcattc tcgacgcgaa tcgctttctc
      961 ggaacccaat tggccgacca accgcgcgtc atctacgccg ccgtcggaaa ggccgtttaa
     1021 cccatgccgt acgatctcac cgggcgaacc gccgtcatca ctggggcgag ccagggcctc
     1081 gggcttgcca ttgctcgtgc ctacgtgagc gcaggtgcct cggtgctcat ctgcgcccgc
     1141 gacgcctcga cgattgctgc ggcacgcgag gagctagaga gtctccgaac tggcgcgacg
     1201 cgcgttttcg gggaagccgc cgacgtagcc gacgaaagtg atgtcgcccg cgtcgtcgag
     1261 cgtgctatct cggacttcgg cacgatcgac atcctcgtca acaacgccgg catctacggg
     1321 cccctgggtg cgattgatga gatcgactgg ccggcatggg tgcgtgcgat cgagatcaat
     1381 ctgctcggct ccgtactctt ttgccgggcc gtcgtgcctc atatgaagcg acaggggaaa
     1441 gggaagatca tccagttatc aggaggcggt gcgacgaatc cgttaccgcg catcagcgcc
     1501 tacgcggcat cgaaggcggc catcgtccgc tttgctgaga cgctggccga ggagttgcgg
     1561 gaacatcgaa tcgacgtgaa ctcgattgcg ccgggcgcgc tcaacacgcg gttgctcgat
     1621 gaggtgctag ccgccgggcc ggagcgcgtc ggagcatcgt tctacgagcg ttcgctgaag
     1681 cagaaagaga acggcggcgc cgggctggat gcgggagcgg aactggcgct cttcctggcg
     1741 tcggccgtga gcgatgggat cacgggaaag ctgctcagcg ccgtctggga tccgtaccca
     1801 gaattgccgc aacacctcga tgacgtgacc aacactgaca tttacacact tcgcagaatt
     1861 gtgccgaagg atcgcggcct caaatggggc gtcgattgaa cgtcgcgatc gtcggctgcg
     1921 gtctcatcgg ccaaaagcgt gcgaaagcgc tcggtgcggc aacgctagtc gcctgttgcg
     1981 atgccgctcc cgatcgcgcg gcggcgcttg cgcgtgcgtg cggtgcgaca tcgcaaagcg
     2041 actggcgtga cgtcatcggg cgcgaggacg tcgacatcgt catcgtcgcg acgacgaacg
     2101 aatcactcgc accgattgcg cttgccgcga tcgaggcagg caagcatgtt ctcgtcgaaa
     2161 agccaggcgc tcgttcggtc ggcgagatcg agccgctcat cacagctgca aaggcacgcg
     2221 atgtgcgcgt ccgtgtcggc ttcaaccatc gctatcatcc agcgctgcag aaagcacgtg
     2281 agttaatcaa tgatggtgct tgcggtgacc tgatgttcgt ccgaggacgc tacggccatg
     2341 gtggccgcat cgggtatgac aaggagtggc gcgccgatcc agtgctggcc ggcggcggtg
     2401 agctgatcga ccaaggcgtt cacgtgatcg atctggcgcg ctggttcctg ggcgacttca
     2461 cgcgtgtcga gggctacgcc ggaacctact tctggcagat gccggtcgat gataacgcat
     2521 ttcttctgct acgcaccgag aagaacctcg ctgcgtttct tcacgtgagt tgcaccgagt
     2581 ggaagaacct cttctcgttc gaaatctacg gccgtacggg aaaactggcc atcgacggac
     2641 tgggcggcag ttacggcgtc gagcggctga cgtgctaccg catgttgccg gagatgggac
     2701 ctcctgagac gacgtcgtgg gagtatccgc gcggcgacga ttcctggggg ctcgagttcc
     2761 gtgagtttct cgacgacatt caaaagaagc gcactccatc ggcggggctt gccgatgccc
     2821 gagcagcgct cgatgtggtc cgcaaggtct acgaggcgtc cacgaaatga tcattgcccg
     2881 cagtccgctt cgaatcacgc tcggtggcgg cggcaccgat cttccctcgt actatcgtga
     2941 gcacggcggc taccttctcg cggcggcgat cgacaagtac gtctacgtta cggtcacgcg
     3001 tccgttcacg cctggcatct ttctgaagta ctcgcagctc gagcatgtcg acgatgtaca
     3061 tgacgtacag catccgatca tccgtgaagc gattcggatg ctcaatttca aaacacctca
     3121 gatcgagatc actacgttgg ccgacatccc gtcgggcacg ggtctcggat cgtccgggag
     3181 tttcacgact gcgcttctga aggcgctcta cgcgcatcgg cgcaagacga ttctcccgca
     3241 cgatctggcg cgccttgcct gcgaaatcga aatcgatcgg ctgcaggagc cggtggggaa
     3301 gcaggatcag tacatcgctg cgcacggagg catcacttgc ttccgtttca atccggatga
     3361 gtcggttagt gcgtcaccgt tgccgatctc attcgacacc atggctgatc tcgaagacaa
     3421 cgtgctcctc ttctttacag gattctcgcg gagtgcgggc agtatcctca aagaccagaa
     3481 ggttcgcagc caggctggcg acgacgaaat gatccgcaac ctcgactacg tcaaagatct
     3541 ggcggtgcga agcgaggccg cgctggtcaa tggcaaggtg gccgacttcg gcgtgctgat
     3601 gcacgagcac tgggagcaca agaagcggcg ctccggcgga atgtccaacc cgaaaatcga
     3661 cgagtggtac gagctcggaa ttcagaacgg cgcggtcggc gggaaactcg tcggggccgg
     3721 cgggggaggc ttcctcatgt tttatgcgga agaccgcaac cggctgcgcc acgccatgtc
     3781 gcgtgcgggg cttgaagagg tgcggtttcg cttcgacttc gagggaacga agatcctgct
     3841 ctcatagccg gaacttctca tactttcccg gttgctcggc catgttattc tcaccagaat
     3901 cctctggaga atgatgtcct acacaaagca acatctcgct gaagcgtcgg aggttattgc
     3961 acggcttgac gctgatgcca tcgagcggat ggcgttcatg ctggcggatc ttcgcgagcg
     4021 tgccgggcgc ctcttttttc tcggcgtcgg cggcagcgcg ggcaactgct cgcacgccgt
     4081 caatgatttt cggaagatcg tcgggatcga gtcgtacgca cctaccgata acgtctccga
     4141 gcttaccgcc cgcacgaacg atgagggttg ggaaaccgtt tttgtcgaat ggctgaagac
     4201 gagccgcctg tcatcgaacg acatgctctt catcttctcg gtaggcggcg gcaatctcga
     4261 gaaaaacatc agtccgaacc tcgtccgcgc gttgcaatac gcacagaccg tcggagcgaa
     4321 agtgaccggt gtcgtcggcc gcgatggcgg gtacacggcg aaggttgccg atgtctgcgt
     4381 catcgtcccg acggtgaatc cggaaaacgt cacgccgcac tccgaggcat ttcaggccgt
     4441 gatttggcac ttgctggtat cgcatccggc actcaaggcc agacagacca aatgggaatc
     4501 cacgtcgctt gaaacgagcc gtgtttcttg atcgcgacgg cgtgatcaac cgcgccatcc
     4561 ttcgcgatgg caagccgcat cctcctcaaa cgctcaggga ggtcgagctt gtacccgatg
     4621 cttcatcgtc gctgcagcgg ctgcacgaag agggtttcct gctgatcgtg gtcaccaacc
     4681 aaccggacgt tgcgcgcggc acgcaggaac gatcgaccgt ggaagcaatc aacaaccacc
     4741 ttgccaccga gttgccgatc gacgagttcg tcgtctgcta tcacgacgat gatgctgcgt
     4801 gcgagtgccg caagccgaac cccggaggca tcctcgacgc cgcagctcgc ctcggtatcg
     4861 acctcagttc gtcgttcctg atcggcgatc gatggcgcga tatcgaggcc ggacagcgcg
     4921 ccggctgcag caactatttc atcgactacg gctatgccga gcgccaaccc gcgccgccgt
     4981 tttcgcgcgt acactcgctc gctgaggccg tgcgtggcat cgttacgaga atcgcaacgg
     5041 aggaatcatg aagacgctcc aggatctgaa ggtcaaacta ttcgctgatg gcgccgacca
     5101 agccggcatg ctcgagatgt accgcaagcc gttcgtcaaa gggttcacga cgaatccgac
     5161 gctgatgcga aaggccggca tcaccgacta ccgcgggttc gcccatgaga ttctggcggc
     5221 gatttccgac cggccgattt cctttgaggt tttctcggac gagtttgacg agatggagcg
     5281 gcaggcgcgc gagatcgcca catggggcgc gaacgtctac gtcaagattc cgatcaccaa
     5341 cacgcgccgc gagcctgcat acgagcttgt acgctcgctt tcgcacgacg gtgtgaaggt
     5401 gaacgtgacg gcgatgatga c
//
LOCUS       DATLVW010000327         7775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_13026, whole genome shotgun sequence.
ACCESSION   DATLVW010000327 DATLVW010000000
VERSION     DATLVW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13026"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1152)
                     /locus_tag="VNN25_10115"
     CDS             complement(25..1152)
                     /locus_tag="VNN25_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXH91924.1"
                     /translation="MRNALVLALIVAISLPAAVGQPSRDADLTRIRGEIARLRKRLDD
                     VRTQTRSAEHDLEEADLELSIRTGELQLAVDMQSQLEQQQREIETEVASLTPRIARHK
                     VFLRKRLAALYRLGGLSYLRLLLSIDERRDPVKAVSMLSFLVSHDAHAVTRFQGEREQ
                     LRARYAQLADRHRQLAGVRQMVEERQRAMASARAAKEKVLRTLQSEGSHGEEKLAGLE
                     EKAKRLEHLLDVLSRQNGGTPAAIDIRTVAGALAWPLKGKIVEQFGKQRNPKFSTVTF
                     NNGLKIAAAAGTEVHAVFAGTVLFSQWFKGYGNLVILDHGNRVFSLYGNLKVPSLLVG
                     SHVDAGQTIAGVGESEDMQSGFLYFEIRQDNKPEDPQKWLR"
     gene            complement(1145..1843)
                     /gene="rnc"
                     /locus_tag="VNN25_10120"
     CDS             complement(1145..1843)
                     /gene="rnc"
                     /locus_tag="VNN25_10120"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXH91925.1"
                     /translation="MQSDLSEFESRIGYSFQQRELLIRALTHKSYSHEAKNSASRDNE
                     TFEFLGDSVLGFVIGDLLFRRFPELDEGAMSKMKAYLVSSTSLARKARAYGMGDVLFL
                     GVGEEKSGGRKKDSLLANLFEAMLAAVYLDGGIDPVRSLIQQSFTEDLEAIDADDLLF
                     QDYKTALQERAQGKGLPLPEYNVIDEVGPDHDKRFIVEVRVGSHVTRGEGSSKKEAQQ
                     QAAKHALREFVQDA"
     gene            complement(2045..4249)
                     /locus_tag="VNN25_10125"
     CDS             complement(2045..4249)
                     /locus_tag="VNN25_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXH91926.1"
                     /translation="MKCSRSAARRAALTLLLGLIPAACGRNSVGTPSRSTPVILISID
                     TLRSDHLPAYGYKAVETPNIDALRAESILYAKAYSHVPLTLPSHLSILTGMLPADHGV
                     RDNVGYRLADSVPTLQELLKKNGYSTGAAVSAFVLRHETGIARGFDFFDDDVKPLNGE
                     NVIGRVQRDGGDTLKAARSWLDAQKTRPFFFFLHLYDPHTPYTPPEPYLSRYQNRYDG
                     EIAYADSVVGDLIADLKQQGVYDDALIILLSDHGEGLSEHGEEEHGLLLYREALQIPL
                     IVKLPQQRKKGATVATPVQLVDVFPTILERTATPMPATGHRVGQSLLTFLDGGAVRPI
                     YSETFYPRFHFGWSDLHSLIDGDDHYIRAPGPELYNLASDPGEKKNTIEENRRANVRL
                     RAAIEPFVKEAETPANIDPEEARKLAALGYVGSTVPTKSGEVLPDPKNQMGVFHDIRL
                     AYTYYRNGNEPEALKLTERLLASNGQITDLWDLKSKILVKMGDNHGAMEASKEGLHHV
                     PGAISLLFEVADLALTMGDLDTAQQHAEIAVKIEPGQAHDILSKVWERRKDMAKAEAE
                     AKLAVATTHDPTDALMMLGRIEKSRGHMQQALDYLDKAVVTNGRSTGKHPGLHAARGD
                     LLARLNRNEEAEQEFRAEIQLNPGAPDAYASLVLLLATENRLDEARNLIFEMVKAAPK
                     PHTYTVVAETLTAMGDDPGALYWAYQGQKKYPQDAELRQLPQHVREAAPKRR"
     gene            complement(4357..7407)
                     /locus_tag="VNN25_10130"
     CDS             complement(4357..7407)
                     /locus_tag="VNN25_10130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH91927.1"
                     /translation="MKRHAIPKLLAVIIAALCISLLAISAFAQVQSGNIFGKVQGKDG
                     SALPGVTVTLTGVGAAQNTVTDASGNFRFINLSPGSYSLKAELAGLGSSTRNGIDVNL
                     GRNADVTLTLNPAVAESITVTAGAPLLDVRKTGTGSDVAKVELQNIPTARDPWVILQQ
                     TPGVTMDRNNVGGNESGQQSVYVSKGTTGTQSTWNVDGVNITDFAATGSSPSYYDFDS
                     FEEMQITTGGTDPRIQTAGVQLNMVTKRGTNDFKGSARAFHTSSSYQSSPKIPSEATG
                     YLSRINQINKIDDDGGEVGGPIVKDKLWFWGAYGEQKIDILTATLLASGARFSDRTTL
                     KNENVKVNAQPIASNSLTLVDQYGSKIKLGRNVSTSRFPETGWNQNDTYAHGTGSLTD
                     PTLWKIEDTQLIGQNLYLTGLYSKVQGGFRLIADNGKGCKDFACGVDSLPSYLDLRPK
                     SAGGDGFNAFHRSYLSVDSLRPQKQTRLDGSAFVNTGSLSHELKFGFGTRNADVTSHT
                     AWPGDQIAILFGNGTDENTVEVVRYPKDTYSSKSQDAYIGDTMLFGNLTVQAALRYDQ
                     QKGSVSAGTAPANALVPELLPAETFPGVSGLKWNNLSPRLGLTYAVGADRRTLLRAQY
                     SRYVDQLTSGAVAPVSPGAYSYAYYYFTDLNGDHAAQRNEIDFASLYGVTGFDKNHPT
                     TATAFTRWANHLSAPHTDEIILGGERELMTDFSVGINGTYRKLSNFVGTVAEHTQGAG
                     DYYSPADYVLHAPITAQLPNGKQVQLSYYTLKPGVALPLYKVIRNTPGYSQQYKGLEL
                     TGTKRMSNRWMLRGNLTLQDWTQKVGSAAIVDPTRDKLCSNCDGSEVIIQSTGSGSKG
                     SVFINSKWSASLTGAYQIPVIETSFGFNVNSRQGYSLPYSWGVSTSEGLKHLLVPTDV
                     DSFRNQNVTEVDVRLAKELKLSHVGLTLSIDGFNMLNSNTILQRVVSGRNSAQDLNRS
                     GGASGQCKVVNGVTDCTAALNASANHVSEVLSPRVFRLGARLSF"
BASE COUNT         1433 a         2274 c         2424 g         1644 t
ORIGIN      
        1 tttgctcatg tcgttttgaa ctcgctaccg cagccatttc tgcggatctt cgggcttgtt
       61 gtcctgccgg atctcgaagt agaggaaacc ggactgcatg tcttccgact cgccgactcc
      121 tgcaatcgtc tgaccagcgt cgacgtggct gccgacgagg agagacggta ctttcaggtt
      181 gccgtaaagt gaaaagacgc ggttgccatg atcgaggatc accagattgc cgtatccctt
      241 gaaccattgc gaaaatagaa ctgttcccgc gaacacagca tgcacctcag tgcctgccgc
      301 cgcggcgatt ttcaggccgt tattgaaggt gacggtcgag aattttggat tccgttgctt
      361 cccgaactgc tcgacgatct ttcccttcag cggccaggca agcgctcccg cgacggtgcg
      421 gatgtcgatc gccgccggcg tcccgccatt ctgccgcgac agaacgtcga gaagatgctc
      481 cagccgcttc gccttctcct cgagtccggc aagcttctcc tctccatggc tgccctcgct
      541 ttgtaacgtc cggaggactt tctccttggc tgcccgcgcc gaggccatgg cccgttgccg
      601 ttcctcgacc atctggcgta ctccggcgag ctgacgatgc ctgtccgcga gctgggcata
      661 cctcgcgcgg agctgctcgc gctcgccctg gaagcgcgtc accgcgtgcg catcgtgcga
      721 gacgaggaaa ctgagcatcg acaccgcttt gaccggatcg cgccgttcat cgatcgacag
      781 cagcaggcgc aggtagctga gcccgccgag ccggtacaac gcggcgagac gtttacgaag
      841 gaacactttg tgacgcgcga ttcgcggcgt cagcgaagcg acctcagtct cgatctcgcg
      901 ctgctgctgc tcgagctgcg actgcatgtc gaccgcgagc tgcaactcac cggtgcgaat
      961 gctgagctcg agatcggcct cctcgaggtc atgctcggcg ctgcgggtct gcgtgcggac
     1021 gtcatcgagc cgcttgcgga gtctggcgat ctcgccgcga attcgcgtca gatcggcatc
     1081 gcgtgacggc tgtccaaccg ccgccggcaa gctgattgcg acaatcagcg ccagcaccag
     1141 cgcgttacgc atcctgaacg aactcgcgca atgcatgctt cgccgcctgc tgctgcgctt
     1201 cctttttcga ggagccctcg ccgcgcgtga cgtgactgcc cactcgcact tcgacgatga
     1261 agcgcttgtc gtggtcgggg ccgacttcgt cgatcacgtt gtactccggc agcggaagtc
     1321 ctttgccctg ggcccgctcc tggagcgccg tcttgtaatc ctggaaaagg agatcgtcgg
     1381 catcgatcgc ttcgagatct tccgtgaacg attgctggat gagactccgc accgggtcga
     1441 ttccaccgtc gaggtagacc gctgccagca tggcttcgaa gagattcgcg agcagcgagt
     1501 ccttcttcct gccgcccgac ttctcttcgc cgacgccgag gaaaagcacg tcgcccatgc
     1561 cgtaagcgcg cgccttccgg gccagactgg tggagctgac caggtacgcc ttcatcttcg
     1621 acatggcccc ttcgtcgagc tcgggaaagc ggcggaagag aagatcgccg atgacgaagc
     1681 cgagtaccga gtcgccgagg aactcgaagg tctcgttgtc tcgcgaggcg ctgttcttcg
     1741 cttcgtgcga atacgacttg tgcgtcagcg cgcgaatgag cagttcccgc tgctggaagg
     1801 aatagccgat acgggattca aactcggaga ggtcggattg caatcgcgtg gaagtatacg
     1861 ggagcgagat cagagaacag agatcagaga tcagcgtgca gagatcagcg tgcagagatc
     1921 agggtacaga gatcagaaca gaacttcgcg gccacggtgt tcgtgtcatc ctgagcggag
     1981 ggacacgatg cacagcggcc ttctctctcg tctctgatct ctcgtctctg atctctctct
     2041 ctaatcaccg gcgttttgga gcggcctcgc ggacatgctg cggcaattgg cgcaactcgg
     2101 cgtcctgtgg gtatttcttc tgcccctggt atgcccagta gagcgctccc gggtcgtcgc
     2161 ccatcgcggt gagcgtctcc gccaccaccg tataggtgtg cggcttcggc gccgccttca
     2221 ccatctcgaa gataagattc ctggcttcgt ccagccggtt ctcggtggcg aggagcagga
     2281 ccagcgaagc gtaggcatcg ggcgcgccgg gattcaactg gatctcggcg cggaactcct
     2341 gctccgcttc ttcattccga ttgagccggg cgagcagatc accgcgggcg gcgtggagtc
     2401 cggggtgctt cccggtcgac cgtccgttcg tgacgaccgc cttgtcgagg tagtcgagtg
     2461 cttgctgcat gtggcctcgc gacttctcga tgcggccgag catcatcagt gcgtccgtgg
     2521 ggtcgtgcgt cgtcgcgaca gcaagtttgg cctctgcctc ggccttggcc atatctttgc
     2581 gccgctccca caccttggaa aggatgtcgt gcgcctgccc tggttcgatc ttcacggcga
     2641 tctccgcatg ttgctgcgcc gtgtcgagat cgcccatggt cagcgccagg tcggcgactt
     2701 cgaagaggag ggagatcgcg cccggtacgt ggtgaaggcc ttctttcgaa gcctccatcg
     2761 cgccgtggtt gtcgcccatc tttacgagaa tctttgattt cagatcccac agatcggtga
     2821 tctgcccgtt ggaggcgaga aggcgctcgg tgagtttcag cgcctcgggc tcattgccat
     2881 ttcggtagta ggtgtacgcg agcctgatgt cgtggaagac gcccatctgg ttcttcggat
     2941 caggaagcac ctctccgctc ttggtcggaa ccgtcgagcc gacatagccg agtgccgcga
     3001 gtttcctggc ttcctccgga tcgatgttgg ccggggtttc cgcttctttc acgaacggct
     3061 cgatagcggc gcgcagcctc acgttggcgc ggcggttctc ctcgatcgtg ttcttcttct
     3121 cgcccggatc ggacgccaag ttgtacagct ccggcccggg cgcgcggatg tagtgatcat
     3181 cgccatcgat cagcgaatgc aggtcgctcc agccgaagtg aaaccgcgga tagaaagtct
     3241 cggagtagat cggccggacc gcaccgccat ccagaaacgt caatagcgat tgcccaacgc
     3301 gatgtccggt agccggcatc ggcgtcgcag tccgttcgag gatcgtcgga aagacatcga
     3361 ccagctggac cggcgtcgca accgttgcgc ctttctttcg ttgctggggc agcttcacga
     3421 tcaggggaat ctgcagcgcc tcacggtaga gaagaagacc gtgttcctct tcaccgtgtt
     3481 cgctgagtcc ttcgccatga tcggagagaa gaatgatcag tgcgtcgtca taaacaccct
     3541 gctgtttcag gtccgcaatg agatcgccta ccacggaatc ggcgtaggcg atttctccgt
     3601 cgtagcggtt ctggtagcgg ctgagatagg gctcgggcgg cgtgtagggg gtgtgtggat
     3661 cgtaaagatg aaggaagaag aagaagggcc gcgtcttctg cgcgtcgagc caactccgcg
     3721 ccgctttgag cgtgtcgccg ccatcgcgct gcacgcggcc gatgacgttc tccccgttca
     3781 acggcttgac gtcgtcatcg aagaaatcga acccgcgggc gatgccggtt tcgtggcgaa
     3841 gcacgaaggc ggagaccgcg gcgccggtcg agtaaccgtt cttcttgagt agctcctgaa
     3901 gtgtgggaac ggagtcagcg agccggtagc cgacgttgtc gcgcactcca tggtccgccg
     3961 gcagcatccc cgtgagaatc gacaggtgcg aggggagcgt cagcgggacg tgactgtagg
     4021 ccttcgcata aaggatcgat tcggcacgca gcgcgtcgat gttcggggtc tcgacggcct
     4081 tgtagccata ggcaggaaga tggtcggaac gcagcgtatc gatcgagatc aggatgaccg
     4141 gggtggatcg ggaaggcgtg ccgaccgagt ttcgtccgca ggcggcgggg atcaacccca
     4201 ggaggagcgt aagggcggca cggcgtgcgg ctgagcgaga gcacttcata gttctgggaa
     4261 ggataaagcc tcggtcaacg agacggcttt cctattcaaa aagaaacccg ctggagttgc
     4321 ccccagcggg tttcgatcac aacaaaatcg ggaagactag aagctcagac gcgcgccgag
     4381 gcggaaaacg cgcggcgaga gaacttcgga aacatggttg gcgctcgcgt taagagcagc
     4441 ggtgcagtcg gtaactccgt tcactacttt gcactgaccg gaagcgccgc cggacctgtt
     4501 cagatcctgc gcactgttcc gtccagaaac gacgcgctgc aggatcgtat tggaattcag
     4561 catattgaat ccgtcgatcg agagggtcag gccgacgtgc gagagcttca gttccttcgc
     4621 caaccggaca tcgacttccg tgacgttctg gttgcggaac gagtcgacgt cggtcggaac
     4681 caacaggtgc ttgagccctt cagaggtact gacgccccac gaatacggca aggagtaacc
     4741 ctggcgggag ttgacattga aaccgaacga cgtctcgatc acagggatct gataggcgcc
     4801 ggtcagactg gcggaccact tcgaattgat gaagacgctg cccttggagc cgctgcccgt
     4861 gctctggatg atcacttccg agccgtcgca gttgctgcac agtttgtcgc gtgtcggatc
     4921 gacgatcgcg gcggagccga ccttctgcgt ccaatcctga agggtgaggt tgccgcgcag
     4981 catccagcgg ttcgacatcc gcttggtgcc ggtgagctcc agtcccttgt attgctggga
     5041 atagcccggc gtattgcgaa tgaccttgta cagcgggagg gcaacacccg gcttcaacgt
     5101 gtagtacgac aattgaactt gcttcccatt cggcagctgt gcagtgatgg gagcgtggag
     5161 cacatagtcc gccggtgagt agtagtcgcc tgcgccctgc gtatgttcgg ctaccgtccc
     5221 gacaaagttc gacagcttgc ggtaggtgcc gttgatgcca acgctgaagt cggtcatcag
     5281 ctcgcgctcg ccaccgagga tgatttcgtc ggtgtgcggc gccgagagat ggttggccca
     5341 gcgagtgaac gccgttgcgg ttgtcggatg gttcttatcg aatccggtaa cgccatacag
     5401 ggacgcgaag tcgatctcgt ttcgctgtgc agcgtggtcg ccattgagat cagtgaaata
     5461 gtagtaggcg taagagtacg cgccaggcga taccggcgcc acagcgccgc tcgtgagctg
     5521 gtcgacatac cggctatatt gagcgcgaag caacgtgcgc cggtcggcac cgaccgcgta
     5581 ggtcaatcca aggcgtggag agaggttgtt ccacttcagg cccgacactc ccgggaaggt
     5641 ttccgcaggg agaagctcag ggacgagcgc gttcgccggc gcggttcctg ccgagacgga
     5701 tcccttctgc tggtcgtaac ggagtgctgc ctgcacggtg agattcccga agagcatcgt
     5761 gtcgccgatg taggcatcct gcgacttcga ggagtacgta tcctttggat aacgaaccac
     5821 ttccactgta ttttcgtcag tgccgttacc gaaaaggatg gcaatttgat cacccggcca
     5881 tgcagtgtgc gaggtgacat cggcattgcg ggtaccgaag ccgaacttca actcgtgact
     5941 gagagatccg gtgttgacga aagcggaacc atcgagacgg gtctgcttct gcgggcgaag
     6001 gctgtcgaca ctcaggtaag agcgatgaaa tgcgttgaat ccgtctcctc ccgcgctctt
     6061 gggtctgaga tcgagatacg acgggagcga gtcgacgccg cacgcgaagt ccttgcaccc
     6121 tttgccgttg tcggcgatca gccggaaacc gccctgcacc ttcgagtaga gtcccgtcag
     6181 gtagaggttc tggccgatga gctgcgtgtc ttcgatcttc caaagggtcg gatcggtgag
     6241 actacctgtg ccatgggcgt aggtatcgtt ctggttccag ccggtctcgg ggaaacgcga
     6301 cgtgctgacg ttgcgtccga gtttgatctt cgagccgtac tgatcgacga gggtcaggct
     6361 gttcgacgcg atcggctgcg cattgacctt gacgttctcg ttcttcagcg tcgtcctatc
     6421 cgagaaacgc gcgccgctgg cgagaagagt ggcagtgaga atatcgatct tctgctcgcc
     6481 gtaggcgccc cagaaccaga gcttgtcctt gacgatcggt ccaccgactt cgccgccgtc
     6541 atcgtcgatc ttgttgatct ggttgatgcg gctgagatag cccgtggcct cggacggaat
     6601 cttcgggctc gactggtacg agctgctggt gtggaatgcg cgcgccgaac ctttgaagtc
     6661 gttcgtgccg cgtttggtga ccatgttgag ctgcacgccc gcagtctgaa tgcgcgggtc
     6721 ggtgccgcct gtcgtgatct gcatttcttc gaaggaatcg aagtcgtagt acgacggcga
     6781 cgagccggtc gcggcgaagt cggtgatgtt cacaccgtca acgttccagg tcgactgcgt
     6841 gccggtcgtc cccttcgaga cgtacaccga ctgctgaccg gattcgttgc cgccgacgtt
     6901 gttgcggtcc atcgtgacgc ccggagtctg ctgcagaatg acccacggat cgcgtgccgt
     6961 cggaatgttc tggagctcca ccttcgccac gtcggagccc gttccggtct tgcggacgtc
     7021 gagcagaggc gcgccggccg tgaccgtgat cgattccgct acggccggat tcagcgtcag
     7081 cgtaacgtcg gcgttgcggc cgaggttgac gtcgatgccg ttacgggtcg agcttcccag
     7141 acccgcaagt tcagccttca ggctgtagct tccgggcgag aggttaatga agcggaaatt
     7201 acccgatgcg tccgtgacgg tattctgggc cgcaccaacg cccgtgagag tgacggtgac
     7261 gccggggagc gcgcttccgt ccttcccttg taccttacca aagatgttgc ctgactgaac
     7321 ctgcgcaaat gcactgatag ccagtagcga gatgcacagc gcggcgatta tgaccgcgag
     7381 taacttggga atggcgtgtc gtttcatatt tctttagctc ctttctttcc tgagaaactc
     7441 ctgaaagatc actggggaac acctctctgc atgcccttgg ccggatgcca ggaatcatgc
     7501 aacacactga cgaagcttga gttagggcga aagtttggaa tctatcgatc gtctgaaatt
     7561 caatgcgccg tagcgccccg cactttttct tggatcgccg gcaagctgat ttccagcccg
     7621 cagtttagca tcagagggct tgtcaacaaa ctgtgattat cgtgagccca gctccgcgag
     7681 cgccttcctg gcagcggcga tgtccgccgc gtggctcacc ttcggagcag tttcgacgta
     7741 ctgcgtgaat gcccgccgcg cctcctcggg atggc
//
LOCUS       DATLVW010000328         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_591877, whole genome shotgun sequence.
ACCESSION   DATLVW010000328 DATLVW010000000
VERSION     DATLVW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591877"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1134)
                     /locus_tag="VNN25_10135"
     CDS             complement(115..1134)
                     /locus_tag="VNN25_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH91928.1"
                     /translation="MSECGVMSENMPMLLTESLDPVQRERTHQHIESCAACASEWSAM
                     KETWQILETLPTVEVPARVKTRFLAEISAASAVPANVVPFRRRSAARWLAEAAAVVIV
                     GGSAFYAGYRTTSHVEPKPGQGGIFQQGMQVAPSYSIAESRVLPAASISPTIEGRPDI
                     QNVQFTDASPSNPQIGVSFDITSHVTVTGKPADKSMVRLLRYVLENEDKMSPSRSRAI
                     DWVRATYSRVDNPDPEITEALANVLRNDEHQGVRIKAADTLQSMQSAMTDGTRDALVE
                     ALKNDPNPAVRVKAVEALAKLARSGAVLDPDMLDTLRKKAAQGDENVYVRVKAAEALS
                     TIQPR"
     gene            complement(1116..1709)
                     /locus_tag="VNN25_10140"
     CDS             complement(1116..1709)
                     /locus_tag="VNN25_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH91929.1"
                     /translation="MKHLSDEELMSVVQAGDFSPASELYDRYSDRIYNFAYRFLKNSE
                     SAEDATQEVFMKMIKHANQFHADARFATWLLTITANHCRDYLRKAENKVTKEGEDVLI
                     TLPAPAERSPHRNLERQQDKKMNEKIIQEALMLLPLDQREVVLLSRFQGLTYAEIAQV
                     AGCSEAAVKSRVFRAIDFLKKAFAGDSRGGNQCLNVG"
     gene            complement(1933..>2277)
                     /locus_tag="VNN25_10145"
     CDS             complement(1933..>2277)
                     /locus_tag="VNN25_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91930.1"
                     /translation="RKHDAPRFRIPHLHNNVIQLLAERGVLAILAYLFLQGLFLRECA
                     RAWGGPATKYAEIGVVVVASLGAAGMFEFNFGDTEVFWIMLDLFAIVIAFTERPEQSN
                     EPRSGVVPPDGP"
BASE COUNT          425 a          679 c          683 g          490 t
ORIGIN      
        1 ctggctcgat ggtgagctgg cgcacgatgc ggttgtttgg ctggaacgtc gtcgccagta
       61 ggtgtgtcgc cggaaacagc gccgcgactg cgatgagaat tcgaatggtt ggtttcatcg
      121 gggttgaatg gtgctcagtg cctctgccgc tttgacgcga acataaacgt tttcgtcacc
      181 ctgcgccgcc ttcttgcgca gcgtatcgag catgtcggga tcgaggaccg cgccgctgcg
      241 ggcaagtttc gcgagcgcct cgactgcctt gacgcgaacc gcgggattgg gatcgttctt
      301 gagcgcttcg acgagcgcgt cgcgggttcc gtcggtcatc gccgactgca tcgattgcag
      361 cgtatccgct gccttgatcc gcacgccctg gtgctcatcg ttgcgcagca cgttcgccag
      421 cgcctcggtg atctccgggt cgggattgtc gacgcgcgag tacgttgcgc gaacccagtc
      481 gatggcacgc gagcgcgacg gcgacatctt gtcctcgttc tccaggacgt agcgcagcag
      541 gcgaaccatg ctcttgtctg ccggcttgcc tgtgacggtt acgtgggacg tgatgtcgaa
      601 cgagacaccg atctgcgggt tggacggcga cgcgtccgta aactgaacat tctggatgtc
      661 gggccgccct tcgatcgtcg gactgatcga cgcggccgga aggacgcgcg attcggcgat
      721 cgagtaggac ggggcgacct gcataccctg ctgaaaaatg ccaccctgcc ccggcttggg
      781 ttccacatgg gacgtggtgc gatacccggc atagaacgca ctcccgccga cgatcacgac
      841 tgccgcggcc tcggccagcc agcgcgcggc gctacggcgc cggaaaggaa cgacgttcgc
      901 cggaaccgcg gaagcggcgg agatctcggc gaggaaacgc gttttcacgc gagccggcac
      961 ctcaacggtc ggcagtgttt ccagaatctg ccaggtttcc ttcattgccg accactcgct
     1021 ggcgcaggct gcgcagcttt cgatgtgctg atgcgtgcgc tcgcgctgaa ccgggtcgag
     1081 tgactcggtg agcagcatcg gcatgttttc tgacatcacc ccacattcag acattggtta
     1141 cctcctcggg aatcgccagc gaaggctttc ttcaggaaat cgatcgctct aaagactctc
     1201 gatttgaccg cagcctcgga acagcctgcg acctgcgcga tctccgcata cgtcagcccc
     1261 tgaaaacgcg agagaagaac gacctcgcgc tgatccagtg ggagcagcat caacgcttcc
     1321 tggatgattt tttcgttcat tttcttgtct tgctgccgtt cgagattccg atgtggcgag
     1381 cgctcggccg gcgcgggaag ggtgatcagc acatcctccc cttccttcgt cactttgttc
     1441 tccgcttttc tcaggtaatc gcggcagtgg ttggccgtaa tcgtgagaag ccatgtcgcg
     1501 aacctggcgt cggcatgaaa ctgatttgca tgcttgatca tcttcataaa aacttcctgt
     1561 gtcgcgtctt cggccgattc gctgtttttc aggaagcggt acgcgaagtt gtagattcga
     1621 tctgaataac ggtcgtaaag ctctgaagcg ggagagaaat cacccgcctg aacgacgctc
     1681 atcaactcct catcgctcag gtgtttcata tcccgtctct cttcttggac gtatccatct
     1741 cggaaaggtt acggacgttc cagaacaacg gcggggacca gttcgctcag gtgcttcata
     1801 tcccggtcct cctcttgttg gacgtagcca tttttgaaag gttacaaacg gtccagaaca
     1861 acagcgggga ccggttcgct caggtgcttc atctcccgtc ctctcttctt cgacgtagcc
     1921 atcgcggaaa ggttacggac cgtccggagg aacaacgccg gaacggggtt cattcgactg
     1981 ctcaggccgc tcggtgaagg cgataacgat cgcgaagagg tcgagcatga tccagaagac
     2041 ctcggtgtcg ccgaagttga actcgaacat ccccgctgcc ccgagactgg cgacgactac
     2101 cacgccgatc tcggcgtatt tcgtggccgg tcctccccag gcgcgcgcac actctcgcag
     2161 gaagagtccc tgcagaaaga ggtacgccag aatggccagc acgccgcgtt ccgccagaag
     2221 ctggatcacg ttgttgtgca ggtgcgggat acggaaacgc ggcgcatcgt gcttccg
//
LOCUS       DATLVW010000329         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_424143, whole genome shotgun sequence.
ACCESSION   DATLVW010000329 DATLVW010000000
VERSION     DATLVW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424143"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VNN25_10150"
     CDS             complement(<1..497)
                     /locus_tag="VNN25_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HXH91931.1"
                     /translation="MSQLAMNHSGLEEGETKFAGRPLLLVGNPNVGKSLLFTRLTNHY
                     VTVSNYPGTTVEMTTAVAEIGDEKRSIIDTPGINSLLPQSQDEIVARDLLLRERDIDV
                     MQVGDIGNMRRTLLLTLQLSEYAVPFVVALNMADELRSMDEGPDVVALSEAVGVPFVS
                     VSALRR"
     gene            755..2494
                     /locus_tag="VNN25_10155"
     CDS             755..2494
                     /locus_tag="VNN25_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXH91932.1"
                     /translation="MHDLTFAFVGAGGDGAVTAGDIIAGVCATEGLQVIKTEAYGPQI
                     RGGESSSTVRVSSKPIYAQGDTVDVLVVFSWADFARFRNELLLAPDAVIFHESGDAPP
                     EELSSRTCHAVPFAALAKEAGAPKSKNVIAVGLVTARYGLSLDVTRHAISRRFAKKPA
                     AVIDANLRAFDRGVMQEETLPQDGKERLEYTYSEPKLLMSGNEASAFAAIHAGCRFFA
                     GYPITPSSEILHFLAEWLPKLGGSSLQTEDELSAIGAVIGASFAGAKAMTATSGPGLS
                     LMSELLGLSSMAEVPAVIVNVQRGGPSTGLPTASEQSDLFHAVYGSHGDTPRVVIACT
                     DVESSFHATVDAFNIAEEYQLPVIVLTDQSIAQRRETIMAGVLEHEVHDRHIAKPDEL
                     EEYRRYRNSANGVSPMSVPGIAGGMYQTNGLEHDEQGRPKSSFAVHEEMNAKRYRKLA
                     AIADRYKMFDRFGAKKPEIGILCWGSSTGVVREALAHMKESDRIGVFAPRMITPLPSH
                     DLQVFIDSCEAILVVEVSYAAQFTQHLRTQVDLPRWKTTVYSRSGGKNLAASEVENEL
                     QRILTNRSQEVAV"
     gene            2491..>3239
                     /locus_tag="VNN25_10160"
     CDS             2491..>3239
                     /locus_tag="VNN25_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXH91933.1"
                     /translation="MTCSPYSPNDYKSDLKPVWCPGCGDFAVLNSVYRALADLHREPH
                     ETVVVSGIGCSSRLPGYVDTYGFNSLHGRALPIATGVKLARPNLTVLAVGGDGDGIAI
                     GGNHFMHAARRNVDIAYFMMDNEIYGLTKGQAAPTTPSGDKTKSTLYGNPEVSVDPCG
                     LAISLGASWVARAFSGDVKGTTELMKLAISHHGFAFLNIISPCVTWRGDDQTKELRAK
                     QRAVPVDHDPTDRTAALRFTNEDDDQMTVGVL"
BASE COUNT          622 a         1043 c          981 g          593 t
ORIGIN      
        1 cgccgcagcg cggagacgga aacgaacggc acacccaccg cctccgagag cgcgacgacg
       61 tccggacctt catccatcga ccgcagttcg tccgccatat tcagcgcgac gacgaacggc
      121 accgcgtact cgctgagttg cagcgtcagc agaagcgtgc gtcgcatatt gccgatgtcg
      181 ccgacctgca tgacatcgat gtccctctcg cgcagcagaa gatcgcgcgc cacgatttca
      241 tcctgcgatt gcggcagcag gctgttgatg ccgggcgtat cgatgatgga gcgcttctcg
      301 tcgccgatct ccgccaccgc cgtcgtcatc tccacggtcg tcccgggata gttcgatacg
      361 gtgacgtaat gattggtgag ccgcgtgaag aggagcgact tgccgacgtt cggattgcca
      421 acaagaagca gcggccgccc ggcgaatttc gtttcgcctt cctcgagtcc tgagtgattc
      481 atcgcaagct gagacatggt ctcagcgacg gtaacgaacc ctcggacatg gggtcgtgac
      541 agttgtcacc tggcgttgtg atggcgactt cctgagtctc accacagaga cacagagggc
      601 acgggttcat ctctgtgatc tctgtgtctc tgtggttgcc ttcttcttca aaaaccgcca
      661 gtcgtgacac tcatcaccag cgtacgtgac actccgcatt cctcgaagtt agcccctcgg
      721 ccatcctcgc agtcgtaatt cgaggagacg cgttatgcac gatttgacgt ttgcgtttgt
      781 cggggctgga ggcgatggag cggtgacggc cggcgacatc attgccgggg tttgtgccac
      841 cgagggcttg caggtaatca agacggaggc gtatgggccg cagatccgcg gcggcgaatc
      901 gtcgtcgacg gttcgcgtca gcagcaagcc catttacgcg caaggcgaca cggtcgatgt
      961 gctggttgtg ttcagctggg ccgacttcgc ccgcttcagg aatgagctgc tcctcgcgcc
     1021 cgacgccgtg atctttcacg aatcaggcga cgcgccgccc gaggagctgt cgtcgcgaac
     1081 ctgccacgcc gtgccgttcg cagcgctggc aaaagaagcg ggcgcaccga aatcgaagaa
     1141 cgtcatcgct gtcggtctcg tcacggcgcg ctacggactc tcgctcgatg tcacccgcca
     1201 cgcgatctca cgccgcttcg cgaagaaacc ggcagccgtg atcgacgcga accttcgtgc
     1261 cttcgaccgc ggcgtgatgc aggaagagac gctgccgcag gatggcaaag agcgcctcga
     1321 gtacacctac tccgagccga agcttctgat gtccgggaac gaggcgtcgg cgtttgcggc
     1381 gatccacgcg ggctgccgct tcttcgccgg ttacccgatc acgccctcgt cggagatcct
     1441 gcacttcctg gccgaatggc tgccgaagct gggcggatca tcgctgcaga ccgaggatga
     1501 gttgtcggcg atcggagccg tgatcggcgc gtccttcgcc ggcgccaaag caatgaccgc
     1561 aacctcgggg cccggactct cgctgatgtc cgagctgctc gggctctctt cgatggccga
     1621 ggttccggcg gtgatcgtca atgtgcagcg cggcggacca tcgaccggct tgccgacggc
     1681 aagcgaacaa tcggatcttt tccacgcggt ctacggaagc cacggcgaca cgccgcgtgt
     1741 cgtcatcgcc tgcaccgacg tcgagagctc gttccacgct acggtcgatg cgttcaacat
     1801 cgccgaggag tatcagttgc cggtgatcgt gctgacggat cagtcgatcg cacagcggcg
     1861 cgagaccatc atggccggcg tgctcgagca cgaagtccac gatcgccaca tcgccaagcc
     1921 tgacgaactg gaggagtacc ggcgctaccg caacagcgcg aacggcgtct cgccgatgag
     1981 cgtgcccggc atcgccggcg gcatgtacca gacgaatgga ctggagcacg acgaacaggg
     2041 acggcccaaa tcgtcgttcg ctgtccatga agagatgaac gcgaagcggt atcgcaagct
     2101 cgccgcgatc gccgaccgct acaagatgtt cgatcgcttc ggcgcgaaga agccggagat
     2161 cggaatcctc tgctggggat cgtcgaccgg cgtcgtgcgc gaggcgctcg ctcacatgaa
     2221 agaaagcgat cgcatcggcg tcttcgcacc gcggatgatc acgccgctgc catcgcacga
     2281 cctgcaggtg ttcatcgact cctgcgaagc aatcctcgtc gttgaagtca gctacgccgc
     2341 ccagttcaca caacatctgc gcacgcaggt cgatctgccg cgatggaaga cgacggtcta
     2401 ctcccgctcc ggaggaaaga acctggccgc atccgaagtc gaaaacgaat tgcaacgcat
     2461 cctcacgaac cgctcgcagg aggtggccgt atgacttgct caccgtattc acccaatgat
     2521 tacaaaagcg atctgaagcc ggtgtggtgt cccggttgcg gcgacttcgc ggttctcaac
     2581 tccgtttatc gcgcgctcgc cgatctgcac cgcgaaccgc atgaaacggt cgtcgtgtcg
     2641 ggcatcggat gctcgtcgcg ccttcccggc tatgtcgata cctacggctt caactcgctg
     2701 catgggcgcg cgttgccgat cgcaacgggc gtcaagctgg cgcgtccgaa cctcaccgtc
     2761 ctcgcagtcg gcggcgacgg cgacggcatc gccatcggcg gcaatcactt catgcacgcc
     2821 gcgcggcgca acgtggatat cgcgtacttc atgatggaca acgaaatcta cgggctgacg
     2881 aaaggtcagg ccgcgccgac aacgccgtcc ggcgataaga cgaagtcgac gctgtacggc
     2941 aaccccgagg tttctgtcga tccctgcggc ctggccatct ccctcggcgc ttcctgggtc
     3001 gcgcgcgcct tctccggcga tgtgaaagga acaaccgagc tgatgaagct ggccatttcg
     3061 catcacggct tcgccttcct caacatcatc tcgccctgcg tcacgtggcg cggcgacgat
     3121 cagacgaaag agctccgcgc aaaacaacgc gccgtccctg tcgatcacga tcccaccgac
     3181 cgcacggcgg cgcttcgctt cacgaacgag gacgacgatc agatgaccgt aggcgtgct
//
LOCUS       DATLVW010000330         3162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_384017, whole genome shotgun sequence.
ACCESSION   DATLVW010000330 DATLVW010000000
VERSION     DATLVW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384017"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1092
                     /locus_tag="VNN25_10165"
     CDS             253..1092
                     /locus_tag="VNN25_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HXH91934.1"
                     /translation="MKFLPAVFEEREIRRLYDDETETWLFSVVDIIQVLTQQPDYQTA
                     RKYWNKLRERLKKEGSQLVTDCHQFKLPANDGKMRLTDVASAETLLRLVQSVPSPRAE
                     PIKVWLAKVGYERMQEMSDPARALDRARETWQKHGRSEKWIQQRMNGQETRNKLTDYW
                     ANHDVKKGQEYAILTNVIHQEWSGVTVKDHKAIKGLKKQNLRDHMSEAELIFTALAEL
                     STRQIAETDAATGMDENKVAAKTGGKIAKRARLELEARTGRKVVTGENYLPPDTTKTP
                     RRR"
     gene            complement(1263..1694)
                     /locus_tag="VNN25_10170"
     CDS             complement(1263..1694)
                     /locus_tag="VNN25_10170"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HXH91935.1"
                     /translation="MKLRHLVLVAVVCAFSLPLLAAPLAKAGRWQSTIKMEMSGMPGK
                     MPPQTFATCLTKEQAENAENLIPKSGDKRGGCTYTDVKVDGSTLSWKMTCAKTGMTGS
                     GTMTYHGESYEGSMEMKMQDRDISATYTGKYVGECDGTEMK"
     gene            complement(1731..3002)
                     /locus_tag="VNN25_10175"
     CDS             complement(1731..3002)
                     /locus_tag="VNN25_10175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HXH91936.1"
                     /translation="MSPLRNLLLLALAGAFAAPAFGAARLTYYSNGVLIPVAWPDSSF
                     PIRYAIDRRVVNAVPQAAAMLDRAAKDWAAVPGTNLGFQSLGTVDGASAGKDGRNTVS
                     MADDLFADQKFIALTTNWYDDQGQIIEADMQIDPTAVSGGYNVQQLVEHEMGHVLGLD
                     HSAVLSSIMYPYVGTGANAVALDSDDRIAISSAYAAAKPAVGGTLEGRVFGDNGGIFA
                     AQVVAVNDQGQAVATALSDKSGNFTLPGIPDGTYRIYAEPLDGPVNVQNLAGFWQSAK
                     VTSFPTHFADGGALRVESGHIYGNISVNGSGVVTLNPKWIGAWAPGGDSASLQAMPAI
                     LHAGSTTTLGVGGDGFISGMTTFDVPSSGFHRISDFKWAGNYVSATFQIDAATPAGSL
                     VVMVKSGNDTAALTGALRVEPSTRSRGVKKG"
BASE COUNT          657 a          914 c          983 g          608 t
ORIGIN      
        1 cacacgagca gcgcatcttc gacatgaaag tcgatgttct tcgcgacctt ggaatgctgt
       61 tattgggcgc ccagctcgtt tacgcgcgca agcgtgagga gaacgagggt tagaagcgtt
      121 tcacgcggag acgcggagat cgcggagggt tctctccccg tcccccgcgt ctccgcgtga
      181 aaccacgcca ggccgtccgc agcgagatgg ctatactgat tgaaactgcc tgcggatgga
      241 acgagctgcc ccatgaaatt cctgcctgct gtattcgagg aacgcgaaat ccgccgtttg
      301 tacgacgacg agaccgaaac atggttgttt tcggtcgttg acatcatcca agttctgacg
      361 cagcagccgg actaccagac ggccagaaag tactggaaca aactccgaga gcgtttgaaa
      421 aaggagggaa gtcaactggt gacagattgt caccagttca aattgccagc caatgacggc
      481 aagatgcgcc tcaccgacgt cgcgagcgcc gagactctgc tgcgtcttgt ccagagcgtt
      541 cccagccccc gagccgagcc tatcaaggtc tggctcgcca aagtcggcta cgagcggatg
      601 caggagatgt ccgaccccgc tcgtgcgctc gatcgcgcgc gcgagacctg gcagaaacat
      661 ggacgcagcg aaaaatggat tcagcagagg atgaacggcc aggaaacccg caacaagctc
      721 accgactact gggcgaacca cgatgtcaag aaggggcagg agtacgccat tctcaccaac
      781 gtcatccatc aggagtggtc cggcgtgacg gttaaggatc ataaggccat caaaggtctt
      841 aaaaaacaga acctgcgtga ccacatgagc gaggccgaat tgatcttcac cgctctggcg
      901 gagctctcca cgcgccagat tgccgagaca gatgcagcca caggcatgga cgaaaacaag
      961 gtcgccgcaa agaccggcgg aaagatcgca aagcgcgcac gactggaact cgaagccaga
     1021 accggcagga aagtcgtgac cggcgaaaac tacctgccgc ctgacacgac caaaactccg
     1081 aggcggcgat gagtgatact gactggagaa gcgtttcacg cggcggcgcg aagaaacctg
     1141 cgcctccgcg cgaaaccact cgctactact tctggttgaa cgcgtaagcg aaggctagct
     1201 ggcgctgcga ccgaacgcgt gatcgcggcc gtcgttgtca attatcaatt gtcaattgcc
     1261 tctcacttca tctcagttcc atcgcactcg cccacgtact tgccggtgta tgtcgcggag
     1321 atgtcgcggt cctgcatttt catctccatc gagccttcgt acgattcgcc gtgatacgtc
     1381 attgtgccgc tgccggtcat tcctgttttt gcgcaggtca tcttccagga cagcgtgctg
     1441 ccgtcgactt tcacgtcggt gtaggtgcag ccgccgcgtt tgtcgcccga cttcgggatc
     1501 aggttttcgg cgttctctgc ctgttctttg gtgagacagg tggcgaaggt ctgcggcggc
     1561 atcttccccg gcatgcccga catttccatc ttgatggtgc tctgccacct tccggctttc
     1621 gccagcgggg ccgcaaggag aggaagtgag aacgcacaca ctacggcaac gagcacgaga
     1681 tgacgaagct tcatgaggat cctccgttgg ttggttcgga ggattatacg tcagcccttt
     1741 ttcacgccgc gggagcgggt ggaaggctcg acgcggagcg cgccggtcag tgcggccgtg
     1801 tcattaccgc tcttcaccat cacaaccaat gatccggccg gcgtcgccgc atcgatctgg
     1861 aacgtagcgc tgacgtagtt gccggcccat ttgaaatcgg agatgcgatg gaagccggaa
     1921 ctgggcacat cgaacgtcgt catcccgctg atgaatccgt cgccaccgac gccgagggtc
     1981 gtcgtgctgc ccgcgtgaag aattgcgggc atggcttgaa gcgaggcgct gtcgccgccc
     2041 ggagcccagg caccgatcca cttcggattc agcgtgacta caccggagcc attcacactg
     2101 atgttcccgt agatgtggcc gctttcgacg cggagagctc cgccgtcggc gaaatgcgtc
     2161 ggaaaggacg tcacctttgc gctctgccag aaaccggcaa gattctgcac attgaccggg
     2221 ccatcgagcg gctctgcgta gatgcggtag gttccatcag gaatgccggg cagagtgaag
     2281 ttgccgctct tgtccgacag tgccgttgcg accgcttggc cctgatcatt cactgccacg
     2341 acctgcgcag cgaaaatgcc gccgttgtca ccgaagaccc gcccttccag cgtgccgccc
     2401 acggcaggtt tcgctgcggc gtaggccgag gagatggcga tgcggtcatc gctgtcgaga
     2461 gccacggcat tcgctccggt tccgacatag ggatacatga tcgatgacag tacggcggag
     2521 tgatcgagcc cgaggacgtg acccatctcg tgctcgacga gctgctgcac gttgtagccg
     2581 ccggagaccg ccgtcggatc gatctgcatg tcggcttcga tgatctggcc ttggtcgtca
     2641 taccagttcg tcgtcagagc gatgaacttc tgatcggcga agagatcgtc ggccatcgag
     2701 acggtgttgc ggccgtcttt gcccgcgcta gcgccgtcga cggtgccgag cgactgaaag
     2761 ccgaggttcg tgccgggaac ggctgcccaa tccttcgctg cgcggtcaag catcgccgcg
     2821 gcctgcggaa ctgcgttcac gacgcgccga tcgatcgcgt agcgaatcgg gaaggagctg
     2881 tcaggccatg ccaccgggat gagaaccccg ttgctgtagt acgtgaggcg cgctgcgcca
     2941 aacgctggag cggcaaacgc gcccgcgagt gcgaggagga gaagattgcg aaggggggac
     3001 atggctgagc ggctgaagtg catctctggt gccattgtaa taacttttgt gtgacttagg
     3061 ataactttgt ttcgagggtg tggggagtcg ggggtcgggg gtaggagaaa gagccggtgc
     3121 gggtgtcgct tttcgggcgt cgggggtcgg agaaagagcc ag
//
LOCUS       DATLVW010000331         4694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_57560, whole genome shotgun sequence.
ACCESSION   DATLVW010000331 DATLVW010000000
VERSION     DATLVW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57560"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VNN25_10180"
     CDS             <1..326
                     /locus_tag="VNN25_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXH91937.1"
                     /translation="EVIDVQTLLPFDVRGVIVESLKKTSRVVFIDEDVPGGATAFMMQ
                     NVLERDRGYDYLDSEPRTLPAKEHRPAYGSDGDYWSKPNRETIFECVYELLHEAMPRR
                     YPRFL"
     gene            complement(411..824)
                     /locus_tag="VNN25_10185"
     CDS             complement(411..824)
                     /locus_tag="VNN25_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91938.1"
                     /translation="MLPTRPESIWHAKVGKSGGVTAYTFDQLMKAIHESGEVNSHTPL
                     SVPANPNVFKPAGEWPAFQKKFGTTPWKVRINGAEYIARGNVELREWAGSKRIAPDTE
                     IFDDYQEEWVKAGSLTELKGAFNAGCLGSFLLGLR"
     gene            1106..1939
                     /locus_tag="VNN25_10190"
     CDS             1106..1939
                     /locus_tag="VNN25_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HXH91939.1"
                     /translation="MGKKPVVFVLMPFDAAFNDVYLLAIKQACLDAGAHAQRVDEQIY
                     QETILQRVYDEIRSADLIVAEMTGRNPNVFYEVGYAHALGKKVILITSEVEDIPFDLT
                     YYPHIVYRGRMAVLREELARRITFLAAQRKAERPRKRKSTRGAPGPSILNGESSEGID
                     HTPAKASVAVALTSNVSERASDFRDVVPEVALTDELSRISFRVQQLQALQLPKDDAEI
                     FKFRTVCIRIDVELRRLEERAVRLDDAQSAERITELREQVAERLNAARVSSEPGTTGG
                     I"
     gene            2153..2926
                     /locus_tag="VNN25_10195"
     CDS             2153..2926
                     /locus_tag="VNN25_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HXH91940.1"
                     /translation="MHFDIEELTLAWRRYRRDLDDRCFVDHPAMVTWIENDLGGWLSD
                     ISTRVDAGYHAQSARRCAVPKPGFMIRPGTVLEPEDAVVYSCLVGRAYAKIHADLLST
                     EGKVDIAYRMSPDPSSIQWTKHDFTVWREWRIKSLAALKRGVTHVVVTDITGFYDNIS
                     IQKLIVELRRLAVDEDDLTLLQESLRKWSSPRDEGIPQGYSASDILAKLYLASFDRRL
                     VRDGYGHLRYVDDIRIFCSGKLEARRAIADLRSTSFKGV"
     gene            2989..3705
                     /locus_tag="VNN25_10200"
     CDS             2989..3705
                     /locus_tag="VNN25_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91941.1"
                     /translation="MADLINELNSRLARELRESPDSPYLRPAEIVSALADADDPPSPV
                     LERAFEDYFVDAPESFDATLFHYLLVRLGKVESRVAVAYCLETLRGKPEETEAILHYL
                     ADVDLEVEELDAMAEYLGSEDAIYDYQLFQVLRWFLARDSVNDRALHLCRVWGSDRNR
                     DPWLQSYAIAYVGRFGDVDDLQEVEQMYSESANDVERADRIGAVRRMERSRRNAFYAR
                     VAGDSELVRRAVKVAKATRR"
     gene            complement(3883..>4694)
                     /locus_tag="VNN25_10205"
     CDS             complement(3883..>4694)
                     /locus_tag="VNN25_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91942.1"
                     /translation="FNADGAALHYSTYLGGSADDAYVRLDGAGNAYVAGTAYSVNFQT
                     TAGAYQTQLRGPSDGFLTKLDPNGAVVFSTFIGGTNRDAITGLDVRNGALYVTGVTSS
                     SDFPTRNPLTAAAPGANSSFAVKFTAGGELVYSTYLGDGVNPDVSLIAVNSRGEAFVL
                     FPVEKNVLPSKDAIQPTLRGAPDLYLLQLDANGSTLQFATYFGGSDNEVAGGLTVDSS
                     DRVVIAGRTSSTDLPLAHAAQAQYAGGTSEAFVAAFQVTPLPPPKRRAVQH"
BASE COUNT          973 a         1293 c         1402 g         1026 t
ORIGIN      
        1 ccgaggtgat cgacgtccag acgctgctcc ccttcgacgt gcgcggcgta atcgtcgagt
       61 cgctcaagaa aacgagccgc gtcgtcttca tcgacgaaga cgtccccgga ggcgccaccg
      121 ccttcatgat gcaaaacgtc ctggaacgcg accggggcta cgactacctc gattccgaac
      181 cccgcaccct ccccgccaaa gaacaccgcc ccgcctacgg ctccgacggc gactactggt
      241 caaagcccaa ccgcgaaacg atcttcgagt gcgtgtacga gctgttgcac gaagcgatgc
      301 cgcggaggta tccgcggttt ttgtagggcc gcgggcgttt tcctcggtgc acagctccac
      361 caccgttcgc tcaaggcctg ccgatggtag accgctcggg atgacccgag ttaccgaagt
      421 cccaatagaa acgacccaag acatccggcg ttaaaagcgc ccttcaattc ggtcagactt
      481 cccgccttca cccactcttc ttggtaatca tcaaaaatct cagtatccgg cgcgatccgt
      541 ttcgaaccag cccactctct tagctcaaca tttccgcgcg ctatgtattc ggcgccgttg
      601 attcttacct tccacggtgt ggttccgaac ttcttctgga acgctggcca ttcgcccgcg
      661 ggtttaaaga cattcggatt ggcaggaacc gagagaggag tgtgactgtt cacctcgccg
      721 ctctcgtgga tcgctttcat cagctgatcg aaggtgtagg cggtgacgcc gccgctcttg
      781 ccgacctttg catgccatat agattcaggt cttgtaggta gcatgcggag attctacgcc
      841 ttcacgagct tgagtttcgt ctcaggagcc ttccgcgtct tcgagatcac accttcggtt
      901 gaagcttggg gtccagcgcc cacgctgcag gcgaagggtg gatcttgcat tcctgattta
      961 actgttcgca gttaacgggt gtggtgcttc ggttatgaga tgctgttttg ggagctttgg
     1021 cttagcagag ggttctggtc ggaggtgccg tggcgcacca atcaaacgtt agttctgcgg
     1081 aatgattggc gatagaatcc gacagatggg gaagaagcca gtcgtttttg ttctcatgcc
     1141 gtttgatgcc gccttcaacg acgtctacct tctcgcgatc aagcaagcct gcttagatgc
     1201 cggcgctcac gcccagcgtg tcgacgaaca gatttatcag gaaactatcc tgcagcgcgt
     1261 gtatgacgag attcgaagtg cagacctgat cgttgctgag atgactggtc gaaatccgaa
     1321 tgtattctac gaggtaggtt atgcgcatgc gcttgggaag aaggttattc ttatcaccag
     1381 cgaggttgag gacataccgt ttgatctgac gtattatccg catatcgtat atcgtggccg
     1441 aatggcggtg cttcgagagg agttggctcg acgcattacg ttcttggcag ctcagcgaaa
     1501 ggccgagcgt cctcgcaaac gcaagtccac acgtggcgct ccggggccgt caattttgaa
     1561 tggtgagtcg tctgagggga ttgatcatac cccggcgaaa gcttcggttg ccgttgcgct
     1621 aacttcgaac gtgagcgaga gggcatcaga cttcagggat gtcgtgcctg aggtcgcact
     1681 aacagatgag ttgagccgaa tcagctttcg agttcaacag ctacaagcgc ttcaactgcc
     1741 caaagacgat gccgagatct tcaagtttcg gactgtctgt attcgcattg atgttgaact
     1801 gcgccgactc gaagaacggg ccgtgcgtct cgacgacgcg caatcggccg agcggattac
     1861 cgaactcagg gaacaagttg cggagcgttt gaacgctgcc cgcgtgtcga gcgagccagg
     1921 aactactggc ggaatctgac cttggtaagg ctcacttctc tcgatgcgca tgcggccatc
     1981 atcaggctcg tgcgcggtat gcgcgtctgt ggacgttggg tcgggtcgaa agcgaacgtg
     2041 aggcgcggag acatcgtctg gtacgctcct cactcgaagt tggaaccatc aggagcccat
     2101 ccttaggtcg cctcgcggag catcgactgt aacattcccg tacaatcgcg acgtgcactt
     2161 tgacatcgaa gagctaacgc tcgcatggcg gcgatataga cgagatctgg atgatcgttg
     2221 ctttgttgat catccggcaa tggtcacgtg gatcgaaaac gaccttgggg gttggttgag
     2281 tgacatttcg acccgcgttg acgctggata tcacgcgcaa tctgcccgtc gctgcgcggt
     2341 tccgaaacca ggattcatga ttcggcccgg aacggttctg gaacccgagg acgctgtcgt
     2401 ctattcatgc ttggtgggac gcgcatacgc caagattcat gcggatcttc tttcaaccga
     2461 gggtaaggtg gacatcgcgt atcggatgtc gcccgatcca tcgtcgatcc agtggaccaa
     2521 acacgacttt actgtttggc gtgagtggcg catcaagtct ttggccgcgc tgaagcgcgg
     2581 agttacgcac gttgtggtga ctgacatcac gggtttctac gacaacattt cgattcagaa
     2641 gctgattgtc gaactgagac ggctcgcggt agacgaagac gacctcacgc tgctgcagga
     2701 gtcattacga aaatggtcct ctccccgaga tgagggtatt cctcagggct actcagcgag
     2761 cgacattctc gcaaaactct acttggcttc cttcgatcgt aggttggtcc gggacggata
     2821 cgggcatctt cgatacgtcg acgacattcg aattttctgc tcgggtaagc tggaagccag
     2881 gcgagcaatt gccgatctaa gaagcacctc tttcaaaggg gtctgacgcc gcaaagcggg
     2941 aagacgaaaa ttctcaccaa aactgaggcc cgttcaacat ttgatggagt ggccgatctc
     3001 atcaacgagc tcaactcgcg cctggctcgg gagctccgag aaagcccgga ttcaccctac
     3061 cttcgaccgg ccgagattgt ctcagctctt gcggatgctg acgaccctcc gtcaccagtg
     3121 ctcgagagag cattcgaaga ttacttcgta gacgctccgg agtcattcga cgcgacactc
     3181 ttccactacc ttctcgtccg attgggaaaa gtcgaatcca gagtagcagt agcctattgc
     3241 ctggaaacct tgaggggaaa accggaagaa accgaagcga tcctgcacta tctcgccgac
     3301 gtcgatcttg aagtcgaaga gttggacgct atggcggagt acttgggcag cgaagacgct
     3361 atctacgact accagttgtt ccaagtgttg cgatggtttc tagcgcgcga ctccgtgaac
     3421 gatcgcgctc ttcacctctg tcgcgtttgg gggagcgacc gaaacagaga tccgtggttg
     3481 caaagttatg cgattgcata cgtgggcagg tttggcgatg tcgatgacct ccaagaggtc
     3541 gaacaaatgt attccgaaag tgcaaacgat gtcgaacgcg cggaccggat tggcgctgtt
     3601 cgacgcatgg aacgctcgcg acgtaatgcc ttctatgccc gcgttgctgg tgacagtgag
     3661 ctagttcgcc gcgccgtcaa ggtggcaaag gcaacccgca ggtgatgtga ccggacatac
     3721 cagagagtgt aatcggggtc gtatctcgaa aattgactta acgttgctct ggcccacact
     3781 ccagcgaggc gaccggtgtt accgttaagt ggatcttcca agtacgaccc cggcttttcc
     3841 ctccgacgtc tacttttcga ggttcgacct gactttctct gctcagtgct gcacagcgcg
     3901 ccgtttcggc ggggggagcg gggtgacctg gaaggcggcg acgaaggctt cggaggttcc
     3961 tcctgcgtat tgagcctggg cggcgtgggc gagcggcagg tctgtggagc ttgtgcggcc
     4021 ggcgatgacg acacggtcac tgctgtcgac ggtcaggccg ccggcaactt cgttgtccga
     4081 gcccccgaag taggtcgcga attgcagcgt ggaaccgttg gcgtcgagct gcaggaggta
     4141 gaggtccggc gcgccgcgga gcgtaggctg gatggcgtct ttcgacggca gaacattctt
     4201 ctccaccggg aagaggacga aggcttcgcc gcggctgttc acggcaatca aggagacgtc
     4261 cgggttgacg ccgtcgccca gataggtcga atagacaagc tccccgccgg ctgtgaactt
     4321 cacggcaaag gacgaattgg ctccgggcgc cgccgccgtc agcggattgc gcgtgggaaa
     4381 gtcggaacta ctcgtaacgc cggtgacgta cagcgctccg ttccggacgt cgaggccggt
     4441 aatggcgtcc cgattcgtcc cgccgatgaa ggtggaaaag acgaccgcgc cgttcgggtc
     4501 gagcttcgtg agaaagccgt cagaggggcc gcggagctgg gtctggtagg cgccggccgt
     4561 tgtctggaag ttgacggagt aggcggtgcc ggccacgtac gcgttcccgg cgccatccaa
     4621 gcgaacgtac gcatcatcgg cggagccccc caggtaggtg gagtagtgaa gagccgctcc
     4681 gtccgcgttg aacg
//
LOCUS       DATLVW010000332         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_221753, whole genome shotgun sequence.
ACCESSION   DATLVW010000332 DATLVW010000000
VERSION     DATLVW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221753"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1477
                     /locus_tag="VNN25_10210"
     CDS             59..1477
                     /locus_tag="VNN25_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH91943.1"
                     /translation="MSEDSRSTCDTATAPGDDSYADLCSDDNFYIHRIEDGHYLVLSV
                     AKVRPILINETGKQFFDLLLECGTREACHARLFDEDVTDFDAFCAELDLLEFLDREPD
                     KRAIDPERTYSIRRKIFYLQLTNRCNLNCTYCFNVEQRRNVHDLPLEQMKVILDKILP
                     VAKEIKLTGGEPLLYRDLAAVIEHIRAFSEDTVIDLMSNGMSEFSRLDLETLRKVNSF
                     CVSCDRLHGDCGERIGFRVDKFQKNIDYLIKNGWANKVQISSVATAFNEEDLKEVRAY
                     CAANGFFHFVAARCPRSDADDEFTQMPTIESMRRLQRTGLEGTGGAIPVPPKTAIELR
                     DRCGAANTTIGIGPEGDCYPCQGTYFPRFKLGNLLSQRLTEVVDTPLAEEMNRITSPT
                     KKAVCSTCKLRHFCGGPCIGNLYPATGDPGGFSPALCHFAKRVVIDRLVHDVKFDAAA
                     TILEEERVAKPLAEQATREDSV"
     gene            complement(1365..1772)
                     /locus_tag="VNN25_10215"
     CDS             complement(1365..1772)
                     /locus_tag="VNN25_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HXH91944.1"
                     /translation="MPVSNPRGVLVCALPPRDPLWMSQPQILLDSVRDGVRAKHYSLR
                     TEEAYVSWIRRSSSAKKHPSAMGTEEVNQFLTSLAVERKVSAVDAESGTECNPLSIQS
                     PLVSPARPAASPHAPPPGSSRRRRILRHGPSGR"
     gene            complement(1831..>3590)
                     /locus_tag="VNN25_10220"
     CDS             complement(1831..>3590)
                     /locus_tag="VNN25_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXH91945.1"
                     /translation="MEGGDILEPLRDRIVGRVSQEDIYDPLTGDLLASQNDEITDELA
                     NSIQEAGIEQVKIRSGLTCETRRGICRRCYGRNLASGKMVEIGEAVGVIAAESIGEPG
                     TQLTMRTFHVGGTASRVSEQSKHEATNPGKIQFINVSTVTNKNGDLVVINRNGKLLIM
                     DDAPIKKLKKGDSAVLDTTGREKERYALVYGSTLKVHDGQHVEAGTQLVEWDPFTSAI
                     LTEVGGKVTFKDIVEGENVREETDRVTGLTQMVIVESATAEKRTPTITIKSKKGEEKR
                     YLLPTGAHVMVQNGNEVYPGDILAKIPRATTKTKDITGGLPRVVELFEARHPRDKAVI
                     SEVEGTVHHGPVVKGMRRVIIETDEGTRHEYSVPRSVHVNVQEGERVRAGDPLIDGPI
                     DPHDVLAVLGIRELQRYLVDKIQEVYRSQSVAINDKHIEVICRQMLRSVRVTRVGDTD
                     FLVDEQVDRFRFMEENEKVMNRGGEPADGTPLLLGITKASLSTDSFISAASFQETTRV
                     LTEASIAGRVDHLRGLKENVIVGRLIPAGTGMPVYREVYLEKDEPVPVQPNLEDLLRA
                     ETDNEDEEALSLADFDDEQ"
BASE COUNT          662 a         1061 c         1133 g          734 t
ORIGIN      
        1 cttggatgag atgcgccctt cgttgggaga atgaccttgg tgcgcacgag gaggttcgat
       61 gtcggaggat agccgctcaa cgtgcgacac cgcgaccgcc ccgggggacg actcgtatgc
      121 cgacctctgt tcggatgaca acttctacat tcatcggatc gaggatggcc attacctcgt
      181 tctgagcgtc gccaaggtac gcccgatact catcaacgag accggcaagc agttcttcga
      241 cctcctgctc gagtgcggca cgcgcgaagc ctgccatgcg cgcctgttcg atgaagacgt
      301 cactgatttc gacgcgttct gcgccgagct cgacctgctc gagtttctgg atcgcgagcc
      361 cgacaagcgc gcgatcgatc cggagcggac ttactcgatc agacgtaaga tcttctactt
      421 acagctcacc aatcgatgca atctgaactg cacctactgt ttcaatgtcg agcagcgaag
      481 gaacgttcac gatctgccgc tcgagcagat gaaagtgatt ctcgacaaga ttcttccggt
      541 cgccaaggag atcaagctta ccggtggcga gcctttgctc tatcgggatc ttgcagcggt
      601 gatcgagcac attcgggctt tcagcgagga caccgtcatt gacctcatga gcaacgggat
      661 gtccgagttc tctcggctcg atctcgagac gctccgcaag gtgaacagct tctgcgtcag
      721 ctgcgatcgt ctgcacggcg actgcggtga acgcataggc tttcgcgtgg ataagttcca
      781 gaagaacatt gactacttga tcaagaacgg atgggcgaat aaagtccaga tcagctccgt
      841 cgcgacggcg ttcaacgaag aggatctcaa ggaggtccgt gcctactgcg ccgcgaatgg
      901 cttcttccac ttcgtcgcgg ctcgatgtcc gcgatccgat gccgacgacg aattcaccca
      961 gatgccgacc atcgaatcga tgcgtcgcct tcagcgcact ggccttgagg gcacgggtgg
     1021 agccatcccg gtgccgccca agaccgcaat cgaactgcga gatcgctgcg gagcggcgaa
     1081 cacaacgatc ggcattggac cggaagggga ctgctaccca tgccaaggga cgtacttccc
     1141 acgtttcaag cttgggaact tgttgagcca gcggctcacc gaagtggtcg atacgcctct
     1201 ggccgaggag atgaaccgca tcactagccc aacgaaaaag gcggtctgtt cgacatgcaa
     1261 gctcaggcac ttctgcggag ggccatgcat cgggaacctc tacccggcga ccggggaccc
     1321 aggcggattc tccccggcgc tctgccattt cgccaagcgc gtggtcatcg accgcttggt
     1381 ccatgacgta aaattcgacg ccgccgcgac gatcctggag gaggagcgtg tggcgaagcc
     1441 gctggccgag caggcgacac gagaggactc tgtatagaaa gaggattgca ctcagtgcct
     1501 gattctgcgt ccaccgcgct gaccttccgc tcgacggcca gtgaggttag gaactgattg
     1561 acctcttcgg ttcccatagc agaggggtgc ttcttggcag aggaggagcg gcgaatccac
     1621 gacacgtatg cctcttccgt gcggaggctg tagtgctttg cgcgtacgcc gtcgcgcaca
     1681 gaatccagga gtatctgtgg ttgtgacatc cacagaggat ccctcggagg gagcgcgcag
     1741 acaaggactc cacgagggtt ggagacgggc atcaacaaaa cgggcggcct ttcggccgcc
     1801 cgctcacgtt ggtacgaatg cgatgagaac ctactgctcg tcgtcaaagt cggccagaga
     1861 aagcgcttcc tcgtcctcgt tgtctgtctc ggcgcggagg aggtcttcga ggttgggctg
     1921 gaccgggacg ggctcgtctt tctcgaggta gacctcgcgg tagacgggca ttccggtgcc
     1981 ggcggggatg agtctcccga cgatgacgtt ttccttgagg ccgcgcaggt ggtcgactcg
     2041 gccggcgatg ctggcttcgg tgagaaccct tgtggtctcc tggaatgagg cggcggagat
     2101 gaagctgtcc gtcgagagcg aggctttggt gatgccgagg aggagtgggg tgccgtcggc
     2161 gggctcgccg ccgcggttca tcaccttctc gttctcttcc atgaagcgga agcgatcgac
     2221 ctgctcgtcc acgaggaagt ccgtatcgcc gactctcgtc acacggacag accgcagcat
     2281 ctgacggcag atgacttcga tgtgcttgtc gttgatggca accgactgcg agcggtagac
     2341 ctcctggatc ttgtcgacca ggtagcgctg cagctcgcgg ataccgagga ccgccaggac
     2401 gtcgtgcgga tcgatcggac cgtcgatgag cgggtcgccg gcgcgaacgc gctcgccttc
     2461 ctgcacgttg acgtgcacgg agcgcggaac gctgtactcg tggcgcgtgc cttcgtcggt
     2521 ctcgatgatg acgcggcgca tgcccttgac caccggaccg tgatgcaccg ttccctcaac
     2581 ctcggagatg acagccttgt cgcgcggatg ccgggcttcg aacagctcga cgacgcgcgg
     2641 cagaccgccc gtgatgtcct tcgtcttggt cgtggcgcgc gggatcttgg ccaggatgtc
     2701 gcccgggtag acctcgttgc cgttctgcac catgacgtgg gcgccggtgg gaagcaggta
     2761 acgcttctcc tcgcccttct tcgatttgat ggtgatggtc ggcgtgcgct tctcagcagt
     2821 ggccgactcg acgatgacca tctgcgtgag accagtgacg cggtcggtct cttcgcggac
     2881 gttctcgcct tcgacgatgt ccttgaacgt aaccttgccg ccgacctcgg tgagaatcgc
     2941 cgaggtgaac ggatcccact cgacgagctg ggtgccggct tcgacgtgct gtccgtcgtg
     3001 aaccttcaga gtggaaccgt agacgagcgc gtaacgctcc ttctcgcgac cggtcgtgtc
     3061 gagcaccgca ctgtcgccct tcttgagctt cttgatgggc gcatcgtcca tgatcaacag
     3121 cttgccgtta cgattgatga cgacgagatc gccgttcttg ttcgtgacgg tggagacgtt
     3181 gatgaactga atctttccgg ggttggtcgc ttcgtgcttc gactgctcgg aaacgcgtga
     3241 tgcggtaccg ccgacgtgga aggtacgcat cgtgagctgc gtgcccggct cgccgatcga
     3301 ctcggcggcg ataacgccga ccgcttcgcc gatctcgacc atcttgccgc tagccaggtt
     3361 gcggccgtag cagcggcgac agatgccgcg gcgggtttcg cacgtcagac ccgaacggat
     3421 cttgacctgc tcgatgccgg cttcctggat ggagttggcc agctcgtcgg tgatctcgtc
     3481 attctgcgac gcgagaaggt cgccggtgag cggatcgtag atgtcttcct gcgagacgcg
     3541 gccgacgatg cgatcgcgga gcggctcgag gatgtcgccg ccttccatga
//
LOCUS       DATLVW010000333         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_509693, whole genome shotgun sequence.
ACCESSION   DATLVW010000333 DATLVW010000000
VERSION     DATLVW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509693"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..227)
                     /locus_tag="VNN25_10225"
     tRNA            complement(138..227)
                     /locus_tag="VNN25_10225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Ser,seq:tga)
     gene            complement(1001..1564)
                     /locus_tag="VNN25_10230"
                     /pseudo
     CDS             complement(1001..1564)
                     /locus_tag="VNN25_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
     gene            1825..>2339
                     /locus_tag="VNN25_10235"
     CDS             1825..>2339
                     /locus_tag="VNN25_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91946.1"
                     /translation="MPCFADAPGVYAITGGTIHPASGPSIDNGAVIIRDGLIESVGSA
                     VAIPLDATIIDVKGGHIYPGLIDAMTSLGFPSASTTRRRGGGGGGARTPPPETLPETG
                     PSFLAFHEAKLTDDDVDAKRATGVLTIVTAPSFGIFNGQSVALNLGGGTMESRVVRNP
                     AAQQISFNPRPA"
BASE COUNT          411 a          680 c          786 g          462 t
ORIGIN      
        1 tgagtacgta tgagtatacg gccgttcggc cgcaatcgcg cgcatccgga ttttgggtcg
       61 caggaccgcc gataagacgg ggttcgggct tcgcggaccc taccatgtca tcaagcaagc
      121 agctaagact cgcgatttgg cggaggaggt gggattcgaa cccacggtac cgtttccggt
      181 acggcggttt tcaagaccgc tgccttaaac cactcgacca ctcctccgtt ggaaggctga
      241 aatctgaagc ctgaaggcag aagtggcctt cggcggttcg gacaacgcca cacctgtcag
      301 caacatttct gaggaagact gaggcaggaa tctacgaaag cagattcacg tccagtgtga
      361 tcttcaggcg cagtctccgg tgctggcgga gctcttcgag ggtgatgttc agggggacgg
      421 tcacttcttt taccaggccg tcggtggcgc ggcgctcggt tggtttggag gcggtggcgg
      481 cgcgtgcagc ggatggcgct tccagttcgg gaagcgagtt caggatcccc tcgggaagcg
      541 ggatctcgat cgacatcatc ggcacgtcgt cggacggttc gctaagcgat ccaaacggcg
      601 agtcggggcc gccgaggatg gagaggccgg acgtcggcgg ttcgagctcg tccgtctttg
      661 gtttcgttgc gtagaaatca aaatcgtcga atggttccgg cgttggaggc gcggtggccg
      721 cggcggcctt cggggccggc ttcgctgcgg cagcggcggc gggcttgggt gtgatcagct
      781 ccgtcgctac catcgggaga tcgtccgcgc cgtgcgcgtc gtcgagcacg aatgacgatg
      841 atgccgcggg tttcgcggtc gggaccggat cgccgaagac gtcatcgtca tcccacaacg
      901 aatcgtgagg acgcgcttcc gcgggagcgg cattcagaat ctgaatctcg cgctcgagcg
      961 gctcggaacc ttccaggccg tatttcttga cgagatgcga caggacgagg tgcgtgatgc
     1021 ccttcaacgt ctcttcgacg ccgacgccgg tcgtggccac cgactcgaac gtcggcacgc
     1081 ccagtttgtt cacctcggtc accatctcgg ccaccggcag cacgttcggc agatcgcgct
     1141 tgttgaattg aatcaccagc ggaatctcgc ggatgcgcac gccctggcgt ttgagattgt
     1201 cctcgaggtt ctgcagcgat tcgagattgg catcggtggc gtggtcctga ctgtcggcga
     1261 cgaagacgat tccgtcggcg cctttgagca cgagctggcg cgtggagttg tagtaaacct
     1321 gccccggaac cgtgtagagc tgcagcttcg tgcgcatgcc gcggatcttg ccgaggtcga
     1381 gggggaggaa gtcgaagaag agtgtgcggt cggtcttcgt cgccagcgaa agcatcttgc
     1441 tcttgttgtt cgacggcagc gagtcataga caaactgcag gttcgtcgtc ttaccgcaga
     1501 ggccgggacc gtagtagacg atcttcgcgg tgatctcttt ggtggcgtag ttgaagagga
     1561 ccatgggagt cgatgagcgt agcgaagggg ggtggaggag tcaatgttgg ggggtggggg
     1621 gtggggagtg gggggtggga gcgcggtcga gagtcagcga ttcagtaacc gagcaaccca
     1681 gcaacgcgtc accggcaacg gaactccgaa ctcctacacc cgacccccca ctccccacac
     1741 cccatcccgt atcatgtcgc gccgctgggg gtacgaatgt ccagacgttt cctctgtttc
     1801 ctgatttttc ttcttcttgc ttcggtgccg tgctttgcgg acgcaccggg cgtttatgcg
     1861 atcacgggcg ggacgatcca tccggccagt ggtccgtcga tcgacaacgg cgcggtgatc
     1921 attcgcgacg gcctgatcga atcggtcggc tccgcggtgg ccatcccgct cgatgccacg
     1981 atcatcgatg tcaagggagg ccacatctat cccggcctca tcgacgcgat gacgtccctc
     2041 gggttcccga gcgcgagcac gacgcgcagg cgaggtggtg gaggtggcgg cgcgcgcaca
     2101 ccgccgcccg aaacactgcc ggagacgggt ccgtcgttcc tcgcgttcca cgaggcgaag
     2161 ctgacggacg atgacgtcga tgccaagcgg gccaccggcg tgctgacgat cgttaccgct
     2221 ccctccttcg gcatcttcaa cggccaatcc gtcgccctga acctcggcgg cgggaccatg
     2281 gaatcgcgcg tggtgcgcaa tccggccgcg cagcagatct cgttcaatcc gcggccggc
//
LOCUS       DATLVW010000334         5167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_384157, whole genome shotgun sequence.
ACCESSION   DATLVW010000334 DATLVW010000000
VERSION     DATLVW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384157"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VNN25_10240"
     CDS             <1..532
                     /locus_tag="VNN25_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH91947.1"
                     /translation="VPFAKSMNLPSTTLVTMSLVVGAVGSAAGRVLSGWMSDGLGRLN
                     VLRLMIGISAVAMPILYLTGNNVALLYVMVFVVYWCFGTQLSVNASTTSDFWGTKNAG
                     MNYGLLFTAYGVAGIIGPKIAGKLFDRYENYQAAFYTAAVLSVIALAFEFLARRPSVP
                     TALRVPAVALGGTPAR"
     gene            592..2703
                     /gene="acs"
                     /locus_tag="VNN25_10245"
     CDS             592..2703
                     /gene="acs"
                     /locus_tag="VNN25_10245"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXH91948.1"
                     /translation="MTITIEKDTLDAVHTDESEIAVHWKEEEYYAPNPEFVQQANLKD
                     PSVSERFAEKNFPQCFEEYADMLTWYERWNTVLDTQDPPFWKWFVGGKINASYNCVDR
                     NLDLYRNKAAIVFVPEPEDEEPQVLTYQELYVRVNEFAALLRSFCGLKAGDRVTIHMP
                     MVAELPITMLACARLGVIHSVVFGGFSGEACGLRAADSGSNVLIYMDGYYRGGKMIDH
                     KASAEIAVATAAKEGQKIDKVLVWKRYPGKYTSATPMVPDRDFFVDEILKDFKRAIVE
                     PVPMDSEAPLFLMYTSGTTGKPKGCQHRTGGYLAYVTGTSKYIQDIQPTDTYWCMADI
                     GWITGHSYIVYGPLALGATSVIYEGLPTYPDAGRVWRIAERLGVNIFHTSPTAIRGLR
                     KAGPDEPMKYNYHFKHMTTVGEPIEPEVWRWYYNIVGKTEAVIVDTWWQTENGGFLCS
                     TKPAIDKMKPGSAGPGVPGIYPVIYDENNQEVKAGSGKAGNICIRNPWPGIFQTIWGD
                     RERFVRQYYSKYCRDTKSKRWQDWPYFAADGAVQAGDGYFRILGRVDDVINVAGHRLG
                     TKELESACLTVVEVAEAAVVPVVDEIKGRVPEIYISLKPGYAANKEISDKVIATIETM
                     IGKIARPRRVHIVPDMPKTRSGKIMRRVLAAISNTLDTGDITTLANPDVVEQIRITVQ
                     GKKKLAFREGPEDLKQFGTQE"
     gene            2814..3452
                     /locus_tag="VNN25_10250"
     CDS             2814..3452
                     /locus_tag="VNN25_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HXH91949.1"
                     /translation="MLVRDVMTSPAVVISPDTTLEDAYRVLHEKQIRHLLVVDHTLEQ
                     THLVGVVTDRDLRLATSTFTLSPHLPASRIADVMCRHPLTADPSDPIEDAAFRMRERK
                     IGCLPVMEDGAVVGIITGIDLLDALLRMTGVDKPSGRLEVRLPDQPGELARLTSFLSH
                     RDVNIHSILSHPEGPWLRTVLRVGSIETRALAHDLREDGFEVVWPAEKPWPR"
     gene            3440..4570
                     /locus_tag="VNN25_10255"
     CDS             3440..4570
                     /locus_tag="VNN25_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HXH91950.1"
                     /translation="MAEVIYHPAYRDYSFGEDHSFSPLRVDMVLDLLGSLGHRVETVA
                     PKQATHREIVDVHSDYYVRRVAALSAGESVPDCEEYGLGTPDNPIFPGMDDAARWLVG
                     GALHGARMIDAGAKRVLQLGGGLHHAQRNTASGFCIYNDLAIAIQHLTRRGLWVAYLD
                     IDVHHGDGVQQILYEDKRVLTMSFHESGQYLFPGTGDVHEIGSGLGRGYKLNVPLQPF
                     TEGESYLEVLQRVVPRALRQFSPAVLVVQAGADAHVDDPLADLMLTTLDYERIFRLIL
                     GWADELTGGRVLFTLGGGYSFRATPRVWTLLYLLLHDLPVPKDLPEPWLTRWSGVMGG
                     QPPTQFHDAPLPANAVPARAEIERRNRQTADRLLEAVRPYWF"
BASE COUNT         1069 a         1650 c         1495 g          953 t
ORIGIN      
        1 ggtcccgttc gcgaagagca tgaatcttcc gagcacgacg cttgtgacga tgagcctggt
       61 ggttggcgcc gtcggaagtg ctgccggccg cgtactgtca ggctggatgt ccgacggact
      121 tggccgcctc aacgtgctgc ggctgatgat cggcatctcc gccgtagcga tgcctatcct
      181 gtatctcacc ggcaacaacg ttgcacttct ctacgtcatg gtgttcgtcg tctactggtg
      241 tttcgggacg cagctctccg tgaatgcatc cacgacctcg gacttctggg gcacgaagaa
      301 cgccggcatg aactacggcc tgctgttcac cgcgtatggc gtcgccggaa tcatcggacc
      361 caagatcgcc ggcaagttgt ttgaccgata cgaaaactat caagcggcct tctataccgc
      421 agcggtactg tcggttatcg ccttggcgtt cgaattcctg gccagacggc cgagcgttcc
      481 gaccgctctg cgcgttcccg ccgttgcatt aggtggaacc ccggcacggt gaccattcgc
      541 ttacaaaaga tcgagaggaa acctctcgca cagcatcaaa gggagacaac gatgacgatt
      601 acgatagaga aggacacact ggacgctgta cataccgatg agtcagagat cgcagtccat
      661 tggaaggaag aggagtacta cgctccaaac cccgagttcg tgcagcaggc gaacctgaag
      721 gatccttcgg tcagcgaacg tttcgcagaa aagaacttcc cgcagtgctt cgaggagtac
      781 gcggacatgc tgacctggta cgagcgctgg aacacggtgc tcgacacgca ggacccgccg
      841 ttctggaaat ggttcgttgg cggcaagatc aacgcttcct acaactgcgt cgatcggaat
      901 cttgacctct accggaacaa ggccgccatc gtctttgtcc ctgagcctga ggacgaagaa
      961 ccgcaggtgc tcacctacca ggagctgtat gtgcgcgtga acgaattcgc cgcactgctg
     1021 cgcagcttct gcgggctcaa agccggcgac cgcgtgacca tccacatgcc gatggtggcc
     1081 gagcttccaa tcaccatgct cgcctgcgcg cgcctcggtg tcatccactc cgtcgtgttc
     1141 ggcggattca gcggcgaggc ctgcggtctc cgcgccgccg actccggcag caacgtcctg
     1201 atctacatgg acggctacta ccgcggcggc aagatgatcg atcacaaggc cagcgcggag
     1261 attgccgtcg ccaccgctgc aaaagaaggg cagaagatcg acaaagtcct cgtctggaag
     1321 cgctatccgg gcaaatacac ctcggccacg ccgatggtgc cggaccgcga tttcttcgtc
     1381 gacgaaattc tcaaagactt caagcgcgcg atcgtcgaac ccgtcccgat ggactccgag
     1441 gcgccgctgt ttctgatgta cacaagcggc accaccggca aaccgaaagg ctgccagcat
     1501 cgcaccggcg gatatctggc ctacgtcacc ggcacgtcca aatacatcca ggacattcag
     1561 cccaccgaca cctactggtg catggccgac atcggctgga tcactggaca ctcgtacatc
     1621 gtctacggtc cgctcgctct cggcgccacg tcggtgatct acgagggatt gccaacctat
     1681 ccggacgcgg gaagagtctg gagaatcgcc gagcgtctcg gcgtgaacat cttccacacc
     1741 tcgcccactg cgattcgcgg actccgcaaa gccggacctg acgaaccgat gaagtacaac
     1801 taccacttca aacacatgac cacggtcggc gagccgatcg agcccgaagt gtggcgctgg
     1861 tactacaaca tcgtcggcaa gaccgaagcg gtcatcgtcg acacctggtg gcagaccgag
     1921 aatggcggct tcctctgcag caccaagccg gccatcgaca agatgaagcc gggcagcgca
     1981 ggaccgggcg ttccgggcat ctatcccgtg atttacgacg agaacaatca ggaggtcaag
     2041 gcgggaagcg gcaaggccgg caacatctgc atccgcaatc cgtggcccgg catcttccag
     2101 acgatctggg gcgaccgcga gcgcttcgtg cgccagtact actcgaagta ctgccgggat
     2161 acgaagagca agagatggca ggattggccg tacttcgccg ccgacggtgc cgtgcaggca
     2221 ggcgacggtt acttccgcat cctcggacgc gtcgatgatg tcatcaacgt cgccggccat
     2281 cgcctcggca ccaaggagct cgagtcggcg tgcctcaccg tggtggaggt ggccgaagcc
     2341 gctgtcgtgc ccgtcgtcga cgagatcaag ggacgcgtac cggagatcta catctcgctc
     2401 aagcccggat acgctgccaa caaggagatc agcgacaaag tcatcgccac gatcgaaacc
     2461 atgatcggca agatcgcgcg gccgcgacgc gtgcacatcg tcccggacat gcccaagaca
     2521 cgttcaggca agatcatgcg ccgcgtcctg gcagcgattt ccaacaccct cgacacgggc
     2581 gacatcacca cgctggccaa ccccgacgtg gtggagcaga ttcgcatcac cgtgcagggc
     2641 aagaagaagc tggcattccg tgaaggaccc gaggatctga agcagttcgg cactcaggag
     2701 tagtcaacac aatcccatcg ctcactcggc gggatccgga tcacggcctc tcagcaacgg
     2761 aggccggtcc ggatctcgct ttttgcgcac gcatacctaa cggagagcgc acaatgttgg
     2821 tccgcgacgt catgaccagt ccggccgttg tcatttcgcc ggacacgact ctcgaggacg
     2881 cctaccgcgt cctgcatgag aaacagatcc ggcacctgct cgttgtcgac catactctcg
     2941 aacaaacgca tctcgtcggc gtggtcacgg atcgcgatct tcgcctggcg accagcacat
     3001 tcaccctttc acctcatctt cccgccagcc gcatcgcgga cgtcatgtgc cgccatccgc
     3061 tgaccgccga tccgtccgat ccgatcgagg acgcagcgtt ccggatgcga gagcgcaaga
     3121 tcggctgtct tccagtgatg gaggatggcg cggtggtggg aatcatcacc ggcatcgatc
     3181 tgctcgacgc gctgctacgc atgaccggcg tggacaaacc ttcgggccgc ctggaggtcc
     3241 ggcttcctga tcagccgggc gagctggcgc ggctgacgag cttcctcagt caccgcgacg
     3301 tcaacattca ttcgatcctc agccaccccg aggggccgtg gctgcgaacg gttctgcgcg
     3361 ttggctcgat cgagacacgc gcactcgcac acgatcttcg ggaggatgga ttcgaggttg
     3421 tctggccggc ggaaaaacca tggccgaggt gatctaccat ccggcgtacc gggactactc
     3481 gttcggagaa gaccactcgt tcagcccgtt gcgcgtcgac atggtgctcg acctgctcgg
     3541 atctcttgga caccgcgttg agaccgtcgc gccaaaacaa gccacccatc gcgagatcgt
     3601 cgacgtccat tccgactact acgtacgccg cgtcgccgcc ctgagcgccg gcgagagcgt
     3661 tcccgattgc gaagaatacg ggctcggcac tcccgacaat ccgatcttcc ccggcatgga
     3721 cgacgccgca cgctggctgg tcggcggtgc actccacggc gcgcgcatga tcgacgccgg
     3781 cgccaaacgc gtgctccaac tcggaggagg actgcaccac gcgcaacgca acaccgcctc
     3841 cggcttctgc atctacaacg atctggcgat cgccatccag catctgactc gccgcggact
     3901 gtgggtcgcc tacctcgaca tcgacgtcca ccacggcgac ggcgtgcaac agattctcta
     3961 cgaggacaag cgcgtcctga ccatgagctt tcatgagtcg gggcagtacc ttttccccgg
     4021 cacgggagac gtacacgaaa tcggatcggg tctgggacgc ggctacaaac tgaacgttcc
     4081 gctgcagccg ttcaccgaag gagagagcta tctcgaggtc ctccagaggg tggtcccgcg
     4141 cgcgctccgg caattctctc ccgcggttct ggtcgtgcag gccggcgcgg atgcacacgt
     4201 cgacgaccct ctcgcggacc tcatgctcac cactctcgat tacgagcgaa tcttccgtct
     4261 catcctcggc tgggccgacg agctcaccgg cggccgcgtc ctcttcaccc tcggcggcgg
     4321 ctactcgttc cgcgcaacac ctcgcgtctg gactctcctc tatcttctac tgcatgacct
     4381 gccagtgccg aaagaccttc cggaaccatg gctgaccaga tggagcggag tgatgggcgg
     4441 ccagcccccc acccaattcc acgacgctcc cctacctgcc aacgccgtcc ccgcacgagc
     4501 ggaaatcgag cgccgcaacc gccagaccgc ggatcgcctg ctcgaagcag tgcgcccgta
     4561 ctggttctga tcggttcgcg gcgcggccca ggctctcgcc ccgaggctgc ggcggaggca
     4621 tgccttcgca ctcttgcgca cacatctgtg ctccaatcgc caggacgcgg caggcgctcc
     4681 ggaggttacg gaggttttcc aacgttcccg tcgatgggcc atatcggccg cggagatgcg
     4741 aagatccgcc tcggaactaa atagttcgcc ttcggaacta attagttcgg cctcggaact
     4801 aaatagctca acctcggaac tcgatagttc gatcagggaa ctaaatagtt cggaccgcgg
     4861 aactacgtag ttcggcctgg gaactaaata gttcgatcgg ggaactaaat agatcaatct
     4921 tggaactaaa tagttccgat ggggaactaa ttagttcgga gggggaacta tttagttcga
     4981 tccgggaact atttatctcg acttgggatc tgtgtagttc ggctttcgac ctatgcagat
     5041 cagcgttgga actagttcga cgatggctaa actactggtt gtacgttagt tatcgttttc
     5101 tcacgcgaag acgcgaagga cgggtagagc gctcaagccg gtaggagccg gtggggtcac
     5161 accttcg
//
LOCUS       DATLVW010000335         7968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_182131, whole genome shotgun sequence.
ACCESSION   DATLVW010000335 DATLVW010000000
VERSION     DATLVW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182131"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VNN25_10260"
     CDS             <1..1319
                     /locus_tag="VNN25_10260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HXH91951.1"
                     /translation="AGDGHGQTIAIVELGGGFSKEDLNTYFAEIGVSVPPVTAVSVVG
                     GSNDPGKDPKADGEVMLDIEVAGAIAPRARQLVYFAKNDDQGFLEALKAAIHDNAVPP
                     AAVSISWGSAESKWTQQAMSEFEQTLMDAASLGVTVCIAAGDDGSSDGTGKLTVDFPA
                     AAPHALSCGGTLLQGSGATITNEEVWNDDGATGGGVSQIFGKPSYQSAIAVPPPAGPK
                     GGRGVPDVSGNAAAGSPYKVRVNGADQVAWGTSAVAPLWAGLIALLSQNLGRRVPFLN
                     PLLYKTQVSINAFRDVTKGNNDNAQLGKYTAKIGWDPCTGFGSPRGAKLLAALKMLQP
                     PTPKPPPPPTPKPPKPHPPKPPNPHPPHPHPPGSSGSTGSTGSLPPLPAFEPASMVGA
                     VPQAGIPFSGGGKAVALVALVGTVAVMGMVATTGILAAVAINKDDQ"
     gene            1316..1576
                     /locus_tag="VNN25_10265"
     CDS             1316..1576
                     /locus_tag="VNN25_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91952.1"
                     /translation="MTDHAKKIVETILRRPDLQAVLRRDTDSLARAIGMRERLREAPS
                     SPEGNAVALATVLSTAAVAGAVAVCGLVSTLAISARSNAKRG"
     gene            1580..2518
                     /locus_tag="VNN25_10270"
     CDS             1580..2518
                     /locus_tag="VNN25_10270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH91953.1"
                     /translation="MAPQPEFSVVIPTRNEGARLAAAVRSIATGRSHEFPLEIVIIDD
                     ASSDGCCGDICALVESWPGLQATVVRLEQWSGIPYARNVGAKEAHADTLIVTDANVEF
                     PLNWDLAIRRARRRNRVLCGTIVDQQSSFRGYGCSLEFPSLGVRWLRDPLAHGGYVPV
                     APCTCTVIAAALFARLGGYDDRMPVYGAAEPEFSVRMWLMGVDVVCLPELLIRHRFRP
                     PSERAPFLDRIRTIQTHNYLRFGILYLSDRQLLQTMQHHALGDPDGFQEALQTVAGGD
                     VWTRRELLRRSFHRSFGWYAMHFGLRDRNGRTLTDS"
     gene            2527..3390
                     /locus_tag="VNN25_10275"
     CDS             2527..3390
                     /locus_tag="VNN25_10275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH91954.1"
                     /translation="MTKRITACIPYYQTRRYVRRAVESLLLQRHRDLTVVVVNDGDPD
                     PPWRELAHINDRRLVRISLPYNYGPYFATAAVLNASPDEYFLIQDADDWSDENRISQL
                     LRVIEHDCSDFAVSRQWWYVEQADGKHRPSDNRKGGMVDGASSPALRYRFPHHGLWRT
                     RSLRMLGGYYGGFRVSYDVLLTNLVLLTGKVSYVAAPLYHYLIRRGSLTTSGTTGLAS
                     PLRAAVWKTISPIYERAYAWQVAMNDCQITSNQLMEWIRQSCRQYVDSTSLAALSDAS
                     ERVAYALRSAR"
     gene            complement(4187..5137)
                     /locus_tag="VNN25_10280"
     CDS             complement(4187..5137)
                     /locus_tag="VNN25_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91955.1"
                     /translation="MSPLRTFVLILVLSSGFASVASGQAVDASVCDVLANPASFDGKF
                     IRIKGAPVVAGFDEFVIDGSGCKPSSAIWFAYPEGTKGKAGPAAFLSLQLAKNSPAVA
                     NATHRTAVTLQKNGDFKQFDSLLATQSKASGACLACPRYTVEATLVGRLDGVSAAGLL
                     RDASGKVTGVAGFGNMNLYRARFVLQSVSSVIPHEVSDTTNAVAARRDSRNAGTSVDP
                     EQAKRAIAAYGEPGEDNGVEVSFGIANEVSPDEGAKGKSASPDGILFRVRFDMNRIGK
                     PMLSRAMAHMGTHIADVRGGVPLSGVDEAESRAWKVTVNE"
     gene            complement(5228..7678)
                     /locus_tag="VNN25_10285"
     CDS             complement(5228..7678)
                     /locus_tag="VNN25_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXH91956.1"
                     /translation="MNTHGTKRLTIVSATFAFALLLALPLQSQSPGTKSGAGTKSGGG
                     KKSGQGSSASATPSTPQIPCARPQQPLVKIPELVSKGGRLRATVTATSEFDRVGTRTP
                     GNINGGPAQPGDPNAYTACYGQWLRAFRSPDAVPPFPTPGTEAAVGDPMNGPTLRARV
                     GDVIELTFLNNIDPGVFGKSIDSGDVGGCDQTFGATAASNYPGTDPKGDKFPNCFHGS
                     TTANIHFHGTHTNPNSTGDNVFLEILSSMRLTGKPVVTPDSVKKDFDAFFDRCEVELG
                     KGSHREWPRQYPSDFPTTTWTDQQKTDLLKFDAQKGGGTPLSPVNDKQIKEGAWPQYY
                     IGSYPYCFKLPAYPDPAASTTSSSTHNHDGTGELAGSAARTLLTEAPHSHLLQMGQSP
                     GTHWYHAHKHGSTAIDVGNGFVGAFIIEGAYDDELSKYYKAANPAWNPSVLVINQFGT
                     SVNLKNPGGGQDKGPDFSVNGRINPVISMRPGEVQMWRIVNGSGRAGTYFIAPPPGFH
                     WRQLAQDGVQLKDVNYQNSKDHPFLLAAGNRADILVQAPAACNITTPPCSYSVQVNNE
                     VDPSDRVGAFPINLLTVNVSGTAFSPVMAFIPTAPAMPDFLKDDIADSEVTGTKTIAF
                     NTIPQNKPPTTPAIHEINGKQFDGEVGAVVLLNTVEEWKITNATQGNISHPFHIHINP
                     FQVTEVFTPNETLANGSPKYVTDKTAMQPGQCYLDPKDPSTWKPCGGSGPSSKDAIWW
                     DVFPIPSGLPATNADGTPINDASGAQIVVPGYFKMRSRFVDYSGYYVIHCHILAHEDR
                     GMMTVVEVAPLASPYAHH"
     gene            complement(7723..>7968)
                     /locus_tag="VNN25_10290"
     CDS             complement(7723..>7968)
                     /locus_tag="VNN25_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91957.1"
                     /translation="ATFSPNPQNVPANSEVTWNNTTEDEHQLTLSDGRVTPVIFAEQS
                     SPEYVIVASITYECTAPDHKGESGSINVVAVQDIPPC"
BASE COUNT         1453 a         2374 c         2538 g         1603 t
ORIGIN      
        1 ctgcgggtga tggccatggc cagacgattg ccatcgtgga actgggcggc ggcttctcca
       61 aggaggatct aaacacgtac ttcgccgaga ttggcgtctc ggtgccgcca gtgacggcgg
      121 tctcggtcgt cggcggttcg aatgatccgg gcaaggaccc caaggctgat ggcgaggtga
      181 tgctggacat tgaagtcgcc ggcgctatcg cgccgcgtgc gcggcaactc gtttacttcg
      241 ccaagaatga cgatcaggga tttctcgagg ctttgaaggc agcgatccac gacaacgcag
      301 ttccgccagc tgcggtctcg atcagttggg gcagcgcgga gtcgaagtgg acccaacaag
      361 ctatgagtga gtttgaacag acgctcatgg atgccgcttc gctcggcgtg accgtctgca
      421 tcgctgcagg agacgacggc tcatccgacg gtaccggcaa actgaccgtc gatttcccgg
      481 cggcagcacc tcacgctctt tcgtgcggcg gaacgctcct ccaaggaagt ggcgcgacga
      541 tcacgaacga ggaagtctgg aatgacgatg gcgctaccgg cggaggggtg agccagatct
      601 tcggcaagcc ttcctatcaa tcggccatcg ccgttccacc gcccgccggg ccgaagggcg
      661 gacgcggagt acccgatgtc agcggcaatg cagcggcagg tagtccctat aaggttcgcg
      721 taaatggcgc ggatcaagtg gcgtggggaa cgagcgctgt tgctcccctg tgggcgggtc
      781 ttatcgccct gctttcacag aatctgggcc gccgcgttcc tttcctcaat cctctgttgt
      841 acaaaacgca ggtctcgatc aacgcctttc gcgacgtcac caagggcaat aacgacaatg
      901 cgcagctggg caagtacacg gcgaagatcg gatgggatcc ctgcacaggc tttggcagtc
      961 cgcggggcgc aaaactcctc gcggctctga agatgttgca gccacctact ccaaaaccgc
     1021 ccccgccacc cacgcctaaa ccaccgaagc cgcatcctcc caagccaccc aatccgcatc
     1081 caccacaccc acaccctcca ggctcgagcg gaagcacagg ttcgacgggc tcacttccgc
     1141 cactgccagc gttcgaaccg gcgtccatgg tgggcgcggt gccacaggct ggaattccgt
     1201 tcagtggcgg cgggaaagct gtcgccctgg tcgcactcgt cggaaccgtg gctgtgatgg
     1261 ggatggtcgc aacgaccggg atcctggctg cggtggccat caataaggac gatcagtgac
     1321 cgaccacgcg aagaagatcg tcgagacgat cctgcgacgc cccgatctgc aggccgtgct
     1381 acgacgggat actgattcgc tcgcgcgagc gatcggtatg cgcgagcgtc tcagggaggc
     1441 gccctcctcg cctgaaggga atgccgtcgc gctggccacg gtcctttcca ccgctgccgt
     1501 ggccggagcc gtcgcggtct gcggtttggt ttcaactcta gcgatcagcg cgcgatccaa
     1561 tgcgaaaagg ggatgagcgt tggcgccaca gccggagttt agtgtcgtca tcccgacccg
     1621 caacgaaggg gcacggcttg ccgcagccgt tcgctcgatt gcaacgggcc gctcgcacga
     1681 attcccgctc gaaatcgtca ttatcgacga cgcctcttca gatggttgtt gcggtgacat
     1741 ctgcgcattg gtcgagtcct ggcctggcct ccaggcgaca gtcgtgcggc tggaacagtg
     1801 gagcgggatt ccatacgcac gcaatgtcgg cgcaaaagaa gctcacgcgg atactctcat
     1861 agtcacagac gcgaacgtcg agtttccact caattgggat ctggcgatac gacgggcgcg
     1921 acgacgcaat cgcgttcttt gtgggacgat cgtcgaccag cagtcgtcgt ttcgaggcta
     1981 tggctgctcg ctcgaattcc cttcactggg cgttcgatgg cttcgcgatc cgttagcaca
     2041 cggcgggtac gttcctgtcg ctccctgtac gtgcacggtt attgctgccg cgttgtttgc
     2101 gcgcctcggc ggatacgacg atcggatgcc ggtctatggt gcagcggagc ctgagttcag
     2161 cgtgcgaatg tggctgatgg gtgttgacgt cgtctgtcta cccgagctgc tcattcggca
     2221 tcggttccgt ccgccctcag aacgtgcgcc gtttctcgat cggatcagga cgatacaaac
     2281 ccacaactat cttcgcttcg ggattctcta cctgagcgac cgccagcttc ttcaaacaat
     2341 gcaacatcat gccctcgggg atcccgacgg attccaagag gcgttacaaa cagtcgccgg
     2401 tggggacgtc tggactcggc gggagctact gagaaggagc ttccatcgtt cgtttggctg
     2461 gtacgcaatg cattttggtc tacgagatcg caatgggcgc acgctcactg attcatgagg
     2521 ctcgcgatga caaaacgaat cacggcttgt atcccttatt accaaacgcg gcggtatgtt
     2581 cggcgggcag ttgaaagcct gctgctccag agacatcgag atttgacggt ggtcgtggtc
     2641 aacgatggcg acccggatcc gccgtggcga gagctcgctc atataaatga tcggcgcctg
     2701 gttcgtattt ctcttccata caattatgga ccctacttcg cgacggctgc ggtactaaac
     2761 gccagcccag atgagtactt tctgatccag gacgctgacg actggagtga cgaaaaccgc
     2821 atttcccaac tgttgcgggt gatcgagcac gactgctctg acttcgctgt atcccgacag
     2881 tggtggtatg tcgagcaggc cgacgggaaa caccggccat ccgataacag aaagggaggg
     2941 atggtggatg gcgcctcatc tccggctctt cgttatcgct tcccccacca cggattgtgg
     3001 cggacgcgtt ctcttagaat gctcggcgga tactatgggg gctttcgagt cagctatgac
     3061 gtactcttga ctaatcttgt tctactcacg ggtaaggtgt cgtatgtcgc ggcgccgctg
     3121 taccactatc tcatacgccg cggatcactc acgacgagcg gaacgaccgg acttgcctct
     3181 ccgttgcgag cggcagtatg gaagaccatc tcgccaattt acgaacgtgc gtatgcatgg
     3241 caggtggcga tgaacgactg tcaaatcacg agcaaccaat tgatggaatg gattcggcag
     3301 agttgtcgtc agtacgtcga ctcgacgtcg ctggcggcgc tctcggacgc gagcgaacgg
     3361 gtcgcctacg cgttacgctc ggcgcgctga gcgaatgcat cgagcccgta acgatcatgt
     3421 ctatgtcagg ccctcattcg atcgcaaact ccccacgtaa gatctcgagg agcgcgccac
     3481 gcagctgact cgtaggccgc taaaggggac gagagcgagg catgaaatgg acgaggtccg
     3541 atcctgtcta cccggtcgcg gacgtcgccg tggcgatcga ctggcacgcg cgcgtcttgg
     3601 gcttcgccgt gacgcttgtg aacccaccgg gtgaggaggt ccctgtctac gccatccgcc
     3661 gccgggaggc agtgtcggtc catcttctgc gaaagggtga cgcgccttat ggcctgacca
     3721 gtccgccacc accgaagtgg ggtcaggtcg aaagtctgaa gtgccggcgc tgtcctgtcg
     3781 ccgtggcgag cccttcaacc caccttagac tttagacccg agagaaggcc tgcggcgatg
     3841 ggccacggct ggtccgcgat cagcggcgcg gtctactggg gaaggttcgg ccgcgcatga
     3901 aaatgtagat tgaacgtcag accggccgac gatcaaccga acaagcggag aagaatcgcg
     3961 ccggcaaggg caccggcgaa catcgcgacg acggacagca ctcgacgcgc cgtgcgcccg
     4021 ccggcgaccg aagagtccgc cgccagctcg gccagcgaac aacgtcagaa ccgtcgtcga
     4081 gccggaacga cgcgtgtctt tggccaagta ggcgagtgcg acgagacgtc aggaccattg
     4141 aattagctgc ccacgtcgag aacggagcac attcgccttc acgctgtcat tcgttgaccg
     4201 tgactttcca tgcgcgcgac tcggcctcgt ccactccgga caggggaaca ccaccgcgga
     4261 catcggcgat gtgcgttccc atgtgcgcca tggccctcga taacatcggc tttccgatcc
     4321 ggttcatgtc gaagcgcaca cggaagagga tcccgtccgg ggaggcgctt ttgccttttg
     4381 caccttcatc cggcgacacc tcattggcaa tgccgaagct cacctcaacg ccattgtctt
     4441 ctcccggttc gccgtaggca gcgatcgctc tcttcgcctg ctccgggtcg acggaggttc
     4501 ccgcgtttcg ggaatcacgc cttgcagcga cggcattggt cgtgtcgctc acctcgtgag
     4561 ggatgacgct ggataccgac tggagcacga agcgcgcgcg atagagattc atattgccaa
     4621 agccggccac tccggtcacc ttcccgctcg cgtcgcgcag gaggccggcc gcgcttaccc
     4681 cgtcaagacg tccaacgagc gtggcctcga ctgtgtaacg aggacaggcc aggcacgctc
     4741 cggacgcctt tgactgcgtc gccagcaatg aatcgaactg cttgaagtcg ccattcttct
     4801 gcagcgtcac tgccgttctg tgggtagcgt tcgcgacagc cgggctgttc tttgcgagtt
     4861 gcagcgacag gaacgccgca ggtcccgcct tccccttcgt cccctcggga tacgcaaacc
     4921 agatcgcgct ggacggcttg caacccgagc catcgatcac gaattcgtcg aatcccgcga
     4981 cgacgggcgc acccttgatc cggatgaact ttccatcgaa cgaagccgga tttgcgagca
     5041 cgtcgcagac cgaggcgtcg actgcctggc ccgaggccac agaagcaaat ccggaggaga
     5101 gaaccaggat taggacgaac gtacggagcg ggctcatata aggtcctttg cgggtcaggt
     5161 cctaagctgc ttccgaggat cttcggccag gcggcggcct cctggccgcc gcctgattcg
     5221 aagcgtctca gtgatgtgcg tagggtgacg ccagcggagc cacttccacg acggtcatca
     5281 tgccgcggtc ttcgtgcgcg aggatgtggc agtggatgac gtagtagccg gagtagtcga
     5341 cgaaacggct gcgcatcttg aagtagccgg ggacgacgat ctgggcgccg ctggcgtcgt
     5401 tgatcggcgt gccgtcggcg tttgtggctg gcagtccgga cggaatcggg aagacatccc
     5461 accagatggc gtccttcgat gagggaccgc tcccgccgca aggtttccag gtgctcgggt
     5521 cctttggatc gaggtagcac tggccgggct gcatcgccgt cttatcggtg acgtatttcg
     5581 gcgagccgtt ggccagcgtt tcgttcggag taaacacctc ggtcacttgg aacgggttga
     5641 tatggatgtg gaacgggtgg gagatgtttc cctgcgtcgc gttcgtgatc ttccactcct
     5701 cgaccgtgtt caacagcacg accgcgccaa cctcgccgtc aaactgcttc ccgttgatct
     5761 cgtggatggc cggcgtggtg ggcggtttgt tctgggggat ggtgttgaag gcgatggtct
     5821 tcgttccggt tacctcgctg tccgcaatgt cgtccttgag gaagtcgggc atggcaggag
     5881 cggtcgggat gaacgccatg accggagaga acgccgtgcc ggagacattg accgtcagca
     5941 ggttgatcgg gaacgcgccg acgcgatccg acggatcgac ctcgttgttg acctgcaccg
     6001 agtagctgca aggtggcgtg gtgatgttgc atgcggcggg agcctgcacg aggatgtccg
     6061 cccgattacc ggccgccagg aggaaggggt ggtctttgct gttctgatag ttcacgtcct
     6121 tgagctggac gccgtcctgc gccagttggc gccagtggaa gccgggaggc ggcgcgatga
     6181 agtacgtacc ggcgcggccc gatccgttga cgatgcgcca catctgcact tcgcccggcc
     6241 gcatggagat gacgggattg atccggccgt tgacggagaa gtcgggtccc ttgtcctgtc
     6301 caccgccggg gttcttcagg ttgacggagg ttccgaactg gttgatgacc agcacggacg
     6361 gattccaggc gggattggca gccttgtagt acttcgacag ctcgtcgtca tacgcgccct
     6421 cgatgatgaa ggcgccgacg aagccgttgc cgacgtcgat ggccgtcgag ccgtgcttgt
     6481 gggcgtgata ccaatgggtg cccggcgact gtcccatctg caaaagatgc gagtgcggtg
     6541 cctcggtcaa tagcgtccgc gccgcgctgc cggccagttc gccggtgccg tcgtggttgt
     6601 gcgtgctgct gcttgtcgtc gatgctgcgg gatcagggta cgccggcaat ttgaagcagt
     6661 acggatagga gccgatgtag tactgcggcc atgcgccctc tttgatctgc ttatcgttca
     6721 ccggtgacaa gggcgtgccg ccgcccttct gcgcgtcaaa cttgaggagg tcggtcttct
     6781 gctggtcggt ccaggtggtg gtgggaaagt cgctggggta ttggcgcggc cactcgcgat
     6841 gcgatccctt ccccagctcc acttcgcagc ggtcgaagaa ggcgtcgaaa tccttcttca
     6901 cgctgtccgg ggtgactacc ggcttcccgg tcagtctcat cgacgagagg atctccagga
     6961 aaacgttgtc gccggtcgaa ttcgggttcg tgtgcgttcc gtgaaagtgg atgttggccg
     7021 tggtcgatcc gtggaagcag ttggggaact tgtctccctt cgggtcggtt cccggatagt
     7081 tcgatgccgc ggtggctccg aatgtctgat cgcacccacc gacgtcgccg ctgtcgatcg
     7141 acttaccgaa cacccctgga tcgatgttgt tgaggaaggt gagctcgatg acgtcgccga
     7201 cccgcgcccg aagcgtcggt ccgttcatcg gatcgccgac ggcggcctct gtcccgggcg
     7261 tgggaaacgg cggaaccgcg tccggactgc ggaaggcgcg gagccactgt ccgtagcagg
     7321 ccgtgtaggc attggggtcg ccgggctggg ccggccctcc attgatgttg cccggagtac
     7381 gggtgccgac gcgatcgaac tcgctggtcg cggtgacggt ggcgcgcagt ctgccgccct
     7441 tggaaacgag ctcggggatc ttcacgagcg gctgctgggg acgggcgcac ggaatctgcg
     7501 gggtcgacgg tgtggcggaa gccgatgagc cctgccccga tttcttcccg ccgccgctct
     7561 tcgttccagc gccgctcttc gtgcccgggg actgactttg tagagggagg gcgagcagca
     7621 gtgcgaatgc gaacgtcgca gatacgatgg tcagacgctt ggtcccatgt gtattcatct
     7681 cttctccctc tctcgctgaa gtggacaagc gacggccgcg gctcagcatg gcgggatgtc
     7741 ctgtacggcg accacattga tcgagccgct ctctcccttg tggtcgggag ccgtgcattc
     7801 atacgtgatc gacgccacga tgacgtactc gggagacgac tgctcggcaa agatgacggg
     7861 cgtaactctg ccgtcgctca gcgtgagctg atgttcgtcc tccgtcgtgt tgttccaggt
     7921 gacttccgaa ttggcaggca cgttttgcgg attcggcgaa aaggtcgc
//
LOCUS       DATLVW010000336         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_141035, whole genome shotgun sequence.
ACCESSION   DATLVW010000336 DATLVW010000000
VERSION     DATLVW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141035"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1617)
                     /locus_tag="VNN25_10295"
     CDS             complement(316..1617)
                     /locus_tag="VNN25_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HXH91958.1"
                     /translation="MTTVPCGLQLQNEPVSARILAHIIVLMIAVSSVALAAPPVIIYN
                     DIPSPLPGNVPSEGFQSDHTAEFGELIQFAGTNRIPTKVNVVMSSFALQSVFTAPGMC
                     PPASLCNASGFSHPITLNLYNVNNSGLVPEPGTLIATRTQTFAIPWRPAGDPACGSIA
                     PNFDPNRWLAGDGKCYTGLAFTIAFDLTSLGITLPNQIIYGIAYNTQTFGSAPIGTNG
                     PFDALNVGLNTAGPSVGSNPGNSDIAYQNTDVGNGGTGTFSDHTGWTGFSVAASFETA
                     DTSVDLSIAKTAPPGVYGTGLPVTYTIAVHNSGPADAAGVTVTDVIPPGSTFSSATPS
                     QGSCGGATSLGTTTITCNLGTLTNGASATIALVLNMPQTPGQVSNTATVSTTNPDTNA
                     ANNSSTATVTVVPASQIPALSGFTLLLLGVAMTLLALIALR"
     gene            complement(1965..3107)
                     /locus_tag="VNN25_10300"
     CDS             complement(1965..3107)
                     /locus_tag="VNN25_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91959.1"
                     /translation="MHTKAQQAGGLATSALILLLAASGCSTNHTPGDGQPSMAPPAAP
                     ASTPGSSSGTSGNPPMTSAYSPGANVDRAEDAAAIMASHQRERYLGPIDPAGVQSGPT
                     PPTGQFIPPSATANPEVTVNASISSPPTPVVISGPDGDAGLFIAPAGTSAGSVGGATA
                     NATIATTAPTTNATLITPTTAAMTATPGQFAAGPAVTQSNIGATTTNTSLSPTMSSAV
                     IPSPNQASSSAGTTISNIATSSRLAQTPTMSSAAIPSPNVAANNTAGTTITNATTTPS
                     RVTLTPTMSSAAIPSPNVAANLRQQTVTATTAPVTKPATISSTRVTAPVASGSLRTGT
                     AAIANSAKSSSTARGRVTLPVRVVSNTDGTLTVTNVSAPLVIQNKQ"
     gene            complement(3301..3516)
                     /locus_tag="VNN25_10305"
     CDS             complement(3301..3516)
                     /locus_tag="VNN25_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91960.1"
                     /translation="MTVRFFPGSRKQKTSLIEGFLTQHHVAHELARPEEFTTKTFHLG
                     TDPAVEVDGRLFIDPNVDALKKIFHVD"
BASE COUNT          671 a         1078 c         1289 g          770 t
ORIGIN      
        1 agcgtggcgt ggcggagaac gatgtcgtag tcggtcttcg gcgtcgtggc gcaggcggcg
       61 aggaggagga cggcaagtgc gagaaggcgt ctcatgttac gcaggagcgt aacaggagtg
      121 tgggcgtctc gcccgcggac cgccgggcgt cctcgcccgg cggtgatgaa tcgtgcgctt
      181 aacgttaacg ccaccggccg ccccgcgcgg aaagcccgat acgaagcggt acacggaaaa
      241 catgatcggg ccatcccgcc cccaagctct cgggcgggac gcccgagagc ccgcgggcga
      301 agacgcccgc actccttatc gcagtgctat tagcgcgagc agagtcatgg ctacgccgag
      361 gagcagcagg gtgaatcccg agagcgcggg gatctgggat gccgggacta cggtcactgt
      421 tgcggtgctg ctgttgttcg cggcgttcgt gtcggggttc gtcgtcgata cggtcgcggt
      481 gttcgacacc tggccgggcg tttgcggcat gttgagcacg agagcgatcg ttgctgatgc
      541 gccgttggtc agtgtgccga ggttgcaggt tatggttgtc gtgcccaggc tcgtggcgcc
      601 gccgcagcta ccctgacttg gggttgcgga gctgaatgtc gagccgggcg ggatcacgtc
      661 ggtgactgtg acgcctgcgg cgtcggccgg cccggagttg tgaacggcga tcgtgtacgt
      721 gaccggcaat cccgttccat aaactccggg aggtgcggtc ttcgcgatcg agaggtcgac
      781 gctcgtgtcg gcagtctcaa agctcgcggc cacgctgaaa ccggtccagc cggtgtggtc
      841 gctgaacgtt cctgtacctc cattgccgac gtcggtgttc tgatacgcga tatccgaatt
      901 tcccggattg cttcccaccg atgggccggc cgtgttgagg ccaacgttca gtgcatcgaa
      961 gggaccgttc gttccgatcg gcgcggagcc gaaggtctgg gtgttgtagg cgatgccgta
     1021 aatgatctgg ttcggaagcg tgatccccaa tgaggtgagg tcgaacgcga tcgtgaaggc
     1081 caggccggtg tagcactttc cgtcgccggc aagccatcga ttcggatcga aattcggcgc
     1141 gatcgagccg cacgccgggt ctcccgcagg ccgccatgga atcgcaaagg tctgcgtgcg
     1201 cgtcgcaatc aacgttccgg gctccggaac caggccgctg ttgttgacgt tgtagagatt
     1261 caacgtgatc ggatggctga accctgacgc gttgcacaac gatgcgggcg ggcacattcc
     1321 cggtgccgtg aagaccgact gcagcgcgaa cgagctcatc accacattca ctttcgtcgg
     1381 aatgcggttc gttccggcga actgaatgag ctcgccgaac tcggcggtgt ggtccgactg
     1441 aaagccttcg ctcggcacat tccccggaag cgggctggga atgtcgttgt agatgatcac
     1501 cggcggcgca gccagggcaa cgctcgaaac ggcgatcatc aggacgatga tgtgcgcgag
     1561 gatgcgcgcg gagacaggtt cgttttgcaa ttgcaaacca caggggacgg tcgtcatgga
     1621 aggctccgga aagttgactg gctgagcgct ttggctcgtc ggatggatgg agttggctga
     1681 aggaccagcg aggtgggagc cggctcaggc tcacgcgcgg gggaggctgg gattcaccgc
     1741 gcgcgcagtt acgctaccac aggatgcgca atttggcagt gagatcggag attcagagac
     1801 aagagatcag agatccagag atcagagaca agcaattcag agcctccaga gattcagaga
     1861 ttcagaaccg gtctctgatc tcttgtctct ggtcgtctct gaatctcttg tctctgatct
     1921 cttgtctctg atctctgctc tctgatctct cgtctcttcc ggcgttattg cttgttctga
     1981 atcacgagcg gcgccgagac attcgtcacg gtcaaggtgc cgtcggtatt cgagacgacg
     2041 cggacaggaa gcgtgacgcg gccacgcgcg gtcgacgatg acttcgcgga gttcgcgatc
     2101 gcagcagtac ccgttcgcag acttccgctc gccaccggtg ccgtaacccg cgtggacgaa
     2161 attgtcgccg gctttgtgac cggcgctgtg gtcgcggtca ccgtttgctg ccgcagattg
     2221 gccgcgacgt tcggactcgg gatcgccgcg gacgacatgg tcggagtcag cgttacgcga
     2281 ctcggagtcg tcgtggcatt cgtaatcgtg gttccggcgg tattgtttgc agccacattc
     2341 ggagagggaa tggccgcaga cgacatcgtt ggcgtctgcg ccaggcggct tgacgtcgcg
     2401 atattcgaga tcgtggttcc ggcggagctg ctcgcttgat tcggagacgg aatcactgca
     2461 gacgacatcg tcggcgacag cgaggtgttc gtcgtcgttg ctccgatatt cgactgtgtg
     2521 actgccggtc ccgcggcgaa ctggcctggc gttgctgtca ttgcggcggt cgtcggtgtg
     2581 atgagagtgg cgttggtggt tggggctgtg gtcgctatcg ttgcatttgc agtggcaccg
     2641 ccgaccgatc cagcgctcgt ccctgcggga gcgatgaaaa ggccggcgtc cccatcagga
     2701 ccgctgatga caaccggagt cggcggcgag ctgatcgacg cgttcaccgt cacctcggga
     2761 ttcgcagtcg cagacggcgg aatgaactgc ccggtgggcg gcgtcggtcc cgattgcacg
     2821 ccggcgggat cgatgggccc gaggtagcgc tcgcgttgat gcgaggccat gatggcggcc
     2881 gcgtcctccg cgcgatcgac gttcgcaccg ggcgagtatg cgctggtcat cggcggattg
     2941 ccggacgtcc ctgaggacga gcccggcgtc gatgccggcg cggcaggcgg cgccatagac
     3001 ggctgcccgt cgcctggtgt gtgattggtc gagcaaccgg acgctgccag aagaaggatg
     3061 agcgcagagg tcgcgaggcc tcccgcctgc tgcgctttcg tatgcataga gcctccagtt
     3121 tctctcgaaa cttcgaggtt attgagagca actggcaggc cagaatgggg ggtggggagt
     3181 gggggtcggg ggtgggaaaa gcactggcta ccagcctggc tcagattggt agcgcgccgg
     3241 agccgatagc gccgaagcgc gaaggctccg acacccgact cccgaaccgc gacccccgcg
     3301 tcaatccacg tggaagattt tcttcaacgc gtcgacgttc ggatcgatga acaggcgtcc
     3361 atcgacttcg actgctggat cggtgccgag gtgaaacgtc ttcgtcgtga actcctcggg
     3421 gcgcgcaagc tcgtgcgcga catggtgctg cgtcagaaag ccttcgatca ggctcgtctt
     3481 ctgttttcta cttccgggga aaaaccttac agtcatcctc cccctccctt gccacccagg
     3541 ttcttttgaa cttcattttg gacggatacg caaacggtat tcctcttttc gcgtgccgtc
     3601 aaataacgaa tgaagggtag tgagccaggc cggctcacca cccgaaggga aggacccgtg
     3661 cgcaacgcga cttatttctt cgcgaacttc gcggggaact gctcgatgat cccgtcgctc
     3721 agaacgtcgg ccatgccgag gatgtgtttg acgacgcgat cgtagtcttt gacgctggcg
     3781 gggtaatcgc ccttcaactg atgcgtcg
//
LOCUS       DATLVW010000337         2829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_509887, whole genome shotgun sequence.
ACCESSION   DATLVW010000337 DATLVW010000000
VERSION     DATLVW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509887"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VNN25_10310"
     CDS             <1..746
                     /locus_tag="VNN25_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXH91961.1"
                     /translation="CRYGASSASMPPSFWMSSDADGHMLGIITVDDVLDVAEQEATED
                     IQKLGGMEALDAPYLQVDVFSMIKKRAGWLSALFLGEMLTATAMGYFETEIAKAVVLA
                     LFVPLIISSGGNSGSQATSLIIRSLALRELRLRDWFRVFGREIISGLALGTLLGFIGF
                     VRIVLWQKLHLKDYGVHYMLVAGTVWFALIGVVLFGTIAGSMLPFILRRLGFDPATAS
                     APFVATLVDVTGLCIYFGVALLILRGTLL"
     gene            complement(781..1284)
                     /locus_tag="VNN25_10315"
     CDS             complement(781..1284)
                     /locus_tag="VNN25_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91962.1"
                     /translation="MPIADDATLLDAYAGKGVLLDSNLLLLLVVGTYDRSQVGKFKRL
                     IMFVPEDFDILRAVVSRFRNVYVTPNTVTEVSNLAGSFSGDARKHCFRVFAATILASE
                     ELIVPSGDAAQHFTFEKLGITDAAMFSVAGNPPLVLTTDFPLSQSLAASGLPVINFNH
                     LRFPYWN"
     gene            complement(1292..1564)
                     /locus_tag="VNN25_10320"
     CDS             complement(1292..1564)
                     /locus_tag="VNN25_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91963.1"
                     /translation="MAQDTIVDVKQAVASAQDYLLSLFPTHSRVELEEVEPSADGGWR
                     ITFSYERDDPDPLGIRRIMVEQGRDFRLYKVVTVDASGNPQSVKIR"
     gene            complement(1613..1993)
                     /locus_tag="VNN25_10325"
     CDS             complement(1613..1993)
                     /locus_tag="VNN25_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH91964.1"
                     /translation="MERVNGIGGIFFKSDDAPALRDWYRNHLGIPVDDSYGGASFEWR
                     EKENQDTVGVTVWSPFPSTTKYFDPTRAPFMINYRVSDLDAMLAQLRNAGAIVDEKIE
                     NTDYGRFGWATDPEGNRFELWQPK"
     gene            complement(2002..>2829)
                     /locus_tag="VNN25_10330"
     CDS             complement(2002..>2829)
                     /locus_tag="VNN25_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91965.1"
                     /translation="LIIAIVIGSIFLIRNLILTANPIAPFLSAAAPHVAGYRAPFLSD
                     YVFEGHFIDESLGASLIAACALSGGALAWILVGVAVLMLLLAPSSRILLPFLVIPAAL
                     SKPPRRFTRIVIGFAIVVQLFLIVYFIDRSGAFSLLSGVVSDSEYLTKQRSSYTSAQT
                     VDAMLPADANVLIVGLNETYWFAHRVRGGGNFDGPRVSLYLEAPAPEALYARLKSDGI
                     THVAIIGPTSAVTAVPQKLAERDTSLTPQAQRMLAQTLDRFASSVSSRGAATVFTLR"
BASE COUNT          591 a          802 c          870 g          566 t
ORIGIN      
        1 cctgcagata cggcgcgtcg agcgcttcca tgccgccgag tttctggatg tcttccgacg
       61 cggatggtca catgctcggc atcatcaccg tcgatgacgt gctcgacgtc gccgagcagg
      121 aagcgacgga ggacattcag aaactcggcg gtatggaagc actcgatgcg ccctaccttc
      181 aggtcgacgt tttctccatg atcaagaagc gtgcgggctg gctgtctgcc ctatttctcg
      241 gggagatgct gacggcgact gcgatgggct acttcgagac ggagattgca aaggccgttg
      301 tcctcgcgct cttcgtaccg ctgatcatct cgagcggcgg gaactcaggc tctcaagcga
      361 cgtcgctgat cattcgctcg ctggcgcttc gggagttgcg cctccgcgac tggttccggg
      421 tcttcgggcg tgagatcatt tccggtctcg cgctcggtac gcttctcggg ttcatcggtt
      481 tcgtccgcat cgtgttgtgg cagaagttgc acctgaagga ttacggcgtg cattacatgc
      541 tggtggcggg gacggtctgg ttcgcactca tcggcgttgt gctcttcggc acgatcgccg
      601 gctcgatgct gccgttcatt ctgcgaagac ttggcttcga tcccgctacc gcgtccgcgc
      661 cattcgtagc aacgctcgtc gacgtgaccg gcctttgcat ctacttcggc gtcgcgctgc
      721 tgattctgcg cggcacgttg ctgtgagggg cagatgcgtg gattcgtgct gggcgctgga
      781 ttagttccag tacgggaacc gaagatgatt gaagttaatc accggcagac cgctggctgc
      841 taacgactgc gacagaggaa aatccgttgt taaaaccaat ggaggattac ctgcaacgct
      901 aaacatggca gcatcagtga ttccgagttt ctcaaacgta aaatgctgcg ccgcgtctcc
      961 actgggtacg attagctctt cggaagcgag aatcgtcgcg gcaaagactc ggaagcagtg
     1021 tttccgcgca tctcccgaga aggaacccgc gagattactg acctcggtga cggtattcgg
     1081 cgtcacatac acgttgcgga aacgcgaaac cacagcgcga aggatgtcga agtcttcagg
     1141 aacaaacatg atcaaacgct tgaatttgcc aacctgagac ctgtcgtacg tcccgacgac
     1201 aagcaatagc agcagattcg aatcgaggag cacgcctttg ccggcgtagg catccaacag
     1261 cgtcgcatca tcggcaatgg gcatgattca atcagcgaat cttcaccgat tggggattgc
     1321 ccgaagcatc cactgtaact accttatata gtcggaaatc tcttccttgc tcgaccatga
     1381 tgcgccggat gccgagtgga tccggatcat cacgttcata gctgaacgta atccgccaac
     1441 cgccgtccgc gctgggttca acctcctcca actctacgcg actatgtgtc gggaagaggc
     1501 tgaggagata gtcctgtgcg ctggcaacag cctgcttaac gtccactatt gtgtcctgtg
     1561 ccatgggtct ttaagtatag cgcacccccg acagcgagac ggtcccagct ccctacttcg
     1621 gctgccacag ctcgaaacga tttccctcgg gatcggttgc ccagccaaac cggccatagt
     1681 cggtgttttc gatcttctcg tcgacgatgg ctccggcgtt tcgtagctgg gcgagcatgg
     1741 cgtcgagatc gctgacccgg taattgatca tgaatggtgc gcgcgtaggg tcgaagtact
     1801 tggtcgtcga tggaaagggc gaccagacgg tcacgccgac ggtgtcctga ttttccttct
     1861 cgcgccactc gaacgacgcg ccgccgtacg agtcgtcgac cgggatgccg aggtgattgc
     1921 gataccagtc gcgcaatgcg ggcgcgtcgt cagacttgaa gaagattcca ccgattccgt
     1981 tgactctttc catcgccgct cctaacgcaa ggtgaatacg gtagctgctc cgcgggacga
     2041 cacgctcgag gcaaaacggt cgagtgtctg cgcgagcata cgctgcgcct gcggcgtgag
     2101 agaagtgtcg cgctcggcca gcttttgcgg aacggccgtg accgcactcg ttggcccgat
     2161 gatcgccaca tgcgtgatgc catcactctt caggcgggcg taaagcgctt ccggtgccgg
     2221 agcctcgaga tagagcgaaa cgcgcgggcc gtcaaaattg ccgccgccgc ggacccgatg
     2281 cgcgaaccaa tacgtctcgt tgagaccgac gatcaagacg ttcgcatccg ctgggagcat
     2341 ggcatcgacg gtctgcgccg acgtgtacga ggagcgctgt ttcgtcaggt actcggaatc
     2401 cgaaacgacg ccggagagga gcgaaaaggc gccactccga tcgatgaaat agacgatgag
     2461 aaagagctgc acgacgatgg cgaagccgat gacgatgcgc gtgaaccggc gcgggggctt
     2521 cgagagggcc gccggaatga cgagaaacgg cagaaggatg cgcgacgacg gggcgagcag
     2581 aagcatcagc accgcgacgc ccacgagaat ccacgcaagc gcgccgccgg aaagcgcgca
     2641 cgcggcaatg agcgaggctc ccaggctttc gtcgatgaaa tgtccctcga agacatagtc
     2701 ggagaggaac ggtgcgcgat agccggcgac atggggcgcc gccgcggaaa gaaagggcgc
     2761 gatcggattt gcagtgagga tgagattgcg gatcaggaag atcgagccga tgacgattgc
     2821 gatgatgag
//
LOCUS       DATLVW010000338         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_509902, whole genome shotgun sequence.
ACCESSION   DATLVW010000338 DATLVW010000000
VERSION     DATLVW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509902"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3871
                     /locus_tag="VNN25_10335"
     CDS             <1..>3871
                     /locus_tag="VNN25_10335"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXH91966.1"
                     /translation="QFSYTIKSSSIDLSKLKLLSSLANILGGNITLTSTGAGTLEQPE
                     LVLDATVNQATLSGLNLPADAPPPNLYIAIRNGQLIVKGSAAGVLTVDGNGSVATDGT
                     LSGLVEVKVTDLAKALAMSPKTASLPASGKLTMNLQLGGKLTSIETLRVDATFPEFDV
                     RVSDHAFTPARPMHLSLHDGRFAFDDFELSQGLGGASPSTFAIGGYAELTGAKRLKID
                     LHGTLEAALLQLFVPDLRAGGQIVVAGNLAGTMAAPRFTGTAEFQNASARIPGFPQAL
                     DAITGTLVFREDRIDIDSIRFRLGGGQIVLGGSVALEGMSPKSVRITMQGTEVAIRYF
                     EGLTLEANFTLLLSGDLERSILTGDVIVNRALYFKDTDIGTALLNAVLARRSVTPVVS
                     ASWQDKVGLRIHLTSDNTLAIRNNIADVTGGADIEVNGTLANPAILGLVTLNEGGKVR
                     FQDIDYRVMRGSINFQNPFRIDPFFDIALEARVSGGISEVESGPIDVTVTLTGTLDRM
                     TPSITSDPPASDITLFSLLGLGALTRNTPTTPLTAGTAGRSLLFQSVSRLINSNGLSL
                     FDSFAISPDAGDLDKTGDPGTKVSFEKQISNTVRILVIYNTRDSRNRVSLEWQVTPDW
                     VLQFTNDELSKEYDIQARFRRRYDGRWVWGSRGRNPFLLLTSSSIAAASVPAPPPISI
                     ATTTVAPASGGIVTSVTFRADSSVDTSTFNQYVVVKIGEPLSIRNVQSSIKSLFATGN
                     FRDIRVDSTPSGNGVAVVFALYTNFRVASIELEGLTGPDRDRAMRALTFHLGDVLSLN
                     AVDHGADAVQEQLKRAGYLDATVDPETTFVRVQSKATVIFHVTRGTVATVGDVIIDGN
                     TSPFTPQQLIGQMKRGPGKKFDLDEARIDGDRMRQWLIRQEYRKASVRYVSNTYDAPA
                     HKVALHYTASAGPIVKVEVTGIPRRSIGGVLPFRRNQPYSGDAIERAAAAIVTTLQQR
                     GFYNATVDTEESLHGNVWTSTFHVNPGQQFHLTAVTFTGNTSIPDKKLQGVVTTSAGG
                     GIKGLIASILRRPQGVTHEQLADDRTTLESYYRLQGFQNATVGTPGVKLNASNGGMTI
                     DFPVTEGPRTMVASVAIEGNEQVATKDLPKLTLKPGDPLSPTIERDDVIALQAFYSDR
                     GNAEVQVRVREEVSTDKASARVVYTIAEGPKISLGDVVVRGNTYTNNNVILRTAELEK
                     GHPFSYTAILEAQQRLYRLGIFQRVDIQPAEASTNVSERNVSISVAEGKDLTVAGSFG
                     ATSPITADTTEKRISPLVSASIAHRNLF"
BASE COUNT          752 a         1348 c         1104 g          667 t
ORIGIN      
        1 cagttctcgt acacgatcaa gtcgtcgtcg atcgatctct cgaaactcaa actgctgtcg
       61 tcgctggcga acatcctcgg cggcaacatc acgctgacgt cgaccggcgc cggcacgctg
      121 gaacaacccg agctcgtcct cgatgcgacg gtcaatcagg ccacgctcag cggactgaat
      181 ctgcccgccg acgccccgcc gccgaacctc tacatcgcca tccgcaacgg tcagctcatc
      241 gtcaaaggat cggctgccgg agtgctgacg gtcgacggca acggcagcgt ggctaccgac
      301 ggcacgcttt ccggactcgt cgaggtgaag gtgaccgacc tcgccaaagc gctggcgatg
      361 tcgccgaaga cggcttcgct tcccgcgtcg ggcaagctga ccatgaatct gcagctcggc
      421 ggcaagctga cctcgatcga gacgctgcgt gtcgatgcca ccttccccga attcgatgtc
      481 cgcgtttcgg atcacgcgtt cacgccggcg cgcccgatgc acctgagcct tcacgatggc
      541 cgcttcgcct tcgatgactt cgagctctcc cagggactcg gcggcgcatc gccatcaacc
      601 ttcgcgattg gtggatacgc cgagctgacc ggcgccaagc ggctcaagat cgatctgcac
      661 ggcacgctcg aggccgcgct gttgcagctc ttcgtgcccg accttcgcgc cggcggccag
      721 atcgtcgtcg ccggcaacct cgccggaacg atggccgcgc cgcgcttcac cggcaccgcg
      781 gaattccaaa acgcctcggc gcgcatcccc ggatttccgc aagcgctcga cgccatcacc
      841 ggcacgctcg tgtttcgcga agaccggatc gatatcgact cgatccgttt ccggctcggc
      901 ggaggacaga tcgttctcgg cggatcggtt gcgctcgaag ggatgtcgcc gaagagcgtg
      961 cgcatcacga tgcagggaac ggaagtggcg atccggtact tcgaaggact gacgctcgaa
     1021 gcgaacttca cgctgctcct cagcggcgat ctcgagcgtt cgatcctcac cggcgacgtc
     1081 atcgtcaatc gcgcgctcta cttcaaagac accgacatcg gcacggccct gctcaacgcc
     1141 gtgctggccc ggcgcagcgt caccccggtc gtcagcgctt catggcagga caaggtcggc
     1201 ctgcgcattc atctcaccag cgacaacacg ctggcgatcc gcaacaacat cgccgatgtc
     1261 accggcggcg ccgacatcga agtcaatgga acgctcgcca accccgccat ccttggcctg
     1321 gtcacgctca acgaaggcgg caaagtacgc ttccaggaca tcgactaccg cgtcatgcgc
     1381 ggctcgatca atttccagaa tccgttccgc atcgatccgt ttttcgatat tgcgctcgaa
     1441 gcgcgcgtga gcggcggcat ctccgaagtc gagtccggcc cgatcgatgt gacggtgacg
     1501 ctgactggaa cgctcgaccg gatgacgcct tcaatcacga gcgatccacc cgccagcgac
     1561 atcacgctct tctcgctcct cggactcggt gcgttgacgc gcaacacgcc caccacgccg
     1621 ctcaccgcag gcacagccgg ccgatcgctg ctctttcagt ccgtcagccg gctgatcaac
     1681 tccaacggat tgtcgctatt cgattcgttc gccatctcac ccgacgccgg cgatctcgac
     1741 aagaccggcg atcccggcac gaaggtctcc ttcgagaagc agatctccaa caccgtccgc
     1801 attctcgtca tctacaacac acgtgactcc aggaatcgcg tctcgctgga gtggcaggtc
     1861 actcccgact gggttctcca gttcacgaac gatgagctct cgaaggagta cgacattcag
     1921 gcccggttcc ggcggcgcta cgacgggcgc tgggtctggg gatcgcgcgg ccgcaatccg
     1981 ttcctgctgc tgacgtcatc gagtatcgcg gctgcgagcg ttcccgcgcc gccgccgatc
     2041 tcgatcgcca cgaccaccgt cgcgccagcg tccgggggaa tcgttacgag cgtcaccttc
     2101 cgcgccgatt cgtcggtcga cacgtccacg ttcaatcaat acgtcgtcgt aaaaatcggc
     2161 gagccgctat cgatccgaaa cgttcagagc tcaatcaaga gcctcttcgc caccggcaac
     2221 ttccgcgaca tccgcgtcga ctccacgcct tccgggaacg gcgtcgccgt cgtcttcgcg
     2281 ctttacacga acttccgtgt tgcgtcgatc gagctcgaag ggctgaccgg acccgatcgc
     2341 gaccgcgcca tgcgcgcgct gaccttccac ctcggcgatg tcctttcgct gaacgccgtc
     2401 gaccacggtg ccgacgccgt acaggagcag ctcaaacggg ccggttatct cgacgccacc
     2461 gtcgatcccg agacgacatt cgtccgcgtg cagagcaaag ccaccgtcat cttccacgtc
     2521 acgcgcggca cggtggcaac ggtcggcgac gtcatcatcg acggcaacac gtctccattc
     2581 acgccgcaac aactcattgg ccagatgaag cgcggtccgg gcaagaagtt cgacctcgac
     2641 gaggcccgaa tcgacggcga tcgcatgcgg cagtggctga ttcgtcagga gtaccgcaag
     2701 gcaagcgtcc gctacgtcag caacacgtat gacgcgcctg cacacaaggt tgcgctccac
     2761 tacacggcgt ccgccggtcc gatcgtgaag gtcgaagtaa ccggcatccc gcgccgttcc
     2821 atcggcggcg tccttccctt ccgccgcaat cagccgtaca gcggcgatgc catcgaacgc
     2881 gccgccgcgg ccattgtgac cacgttgcag caacgcggct tctacaacgc caccgtcgac
     2941 accgaagagt cgctgcatgg caacgtctgg acttcgacct tccacgtcaa tccgggtcag
     3001 cagttccacc tcaccgccgt cacgttcacg ggcaacacat ccattcccga caagaagttg
     3061 cagggcgttg tcacgacatc cgcgggtggc ggaatcaagg gattgatcgc gtcgattctg
     3121 cgccggccgc aaggcgtcac gcacgagcaa ctcgcggacg atcgcaccac gctggagtcg
     3181 tactaccggc ttcagggctt ccagaacgcg acggtcggca cgccgggcgt gaaactcaat
     3241 gccagcaatg gcggcatgac gatcgacttc ccggtcaccg aggggccgcg aacgatggtg
     3301 gcatcggtgg cgatcgaggg gaacgagcag gtcgcgacga aggacttgcc gaagctcacc
     3361 ttgaagccgg gcgacccgct gagtccgacg atcgaacgcg acgacgtcat cgcgctacag
     3421 gcgttctaca gcgaccgcgg caacgccgag gtgcaggtgc gcgtgcgaga ggaagtgagt
     3481 acggataagg cgtcggcgcg cgtcgtctac acgattgcgg aagggccgaa gatcagcctc
     3541 ggcgacgtcg tcgttcgcgg caacacctac accaacaaca acgtcatcct tcgcacagcc
     3601 gagttggaga aagggcaccc attcagttac acggcgattc tcgaagcgca gcagcggctt
     3661 taccggctcg gtatcttcca gcgcgtcgac attcagcctg cggaagcctc gaccaacgtc
     3721 tccgaaagaa acgtatccat ctccgtcgcc gaaggcaaag acctgactgt ggccggttcg
     3781 ttcggcgcca cctctccgat caccgccgac accaccgaga aacgcatctc cccgctcgtt
     3841 tccgcatcga tcgcacaccg caaccttttc g
//
LOCUS       DATLVW010000339         4461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_222411, whole genome shotgun sequence.
ACCESSION   DATLVW010000339 DATLVW010000000
VERSION     DATLVW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222411"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VNN25_10340"
     CDS             complement(<1..340)
                     /locus_tag="VNN25_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXH91967.1"
                     /translation="MRDLHFHALLAATVLAIAGSVGIAAAASPDPALVSAVGSAARSP
                     AAVARDSARHPVEELTFFGLTPTMTVVELWPGGGYWTDILGPYLAPGGHYYIALPAPG
                     NAEEDASVKRL"
     gene            complement(424..1125)
                     /locus_tag="VNN25_10345"
     CDS             complement(424..1125)
                     /locus_tag="VNN25_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXH91968.1"
                     /translation="MSTEHRVMRSIGLVGFCSAVALLAALAGCSGADDGDGSNKINGS
                     IHVTAGKPSGSVATVNGSIHVDDNATITSASTVNGSVHLDGHATADSLTSVNGSITLG
                     AGARVSGAVSSVNGELNLSDSAEVNGTLSNVNGKITLVSAHVAGGIKTVSGSMSITGG
                     SRVEGGILVQKPSGEMLSILHEVPQIVIGPGATVQGELRFERTVKLYVSDKATIGTVT
                     GATPIPFTGDNPPPN"
     gene            1244..1597
                     /locus_tag="VNN25_10350"
     CDS             1244..1597
                     /locus_tag="VNN25_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXH91969.1"
                     /translation="MVNYNTQLDRTFAALVDPTRRAILARLEREDGASVSELAKPFAI
                     KLPAVMKHLDVLVEAGLVTRSKLGRTVTVRLSPQPMRAALDWLRRYERFWSGSLDRLA
                     VYAERKEAEARKRGR"
     gene            1612..2040
                     /locus_tag="VNN25_10355"
     CDS             1612..2040
                     /locus_tag="VNN25_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXH91970.1"
                     /translation="MRRIAARPSIVFEALTSPEGIVHWWGPDAGPVLLAETDVRVGGR
                     FRVRFRTLDGSEHESSGEYLEIVNPARLAMSWRWKGGEDPGESLVEIDLRGIAEGTEL
                     TFTHSRLRDESTRRSHEEGWNGALDKLQYHFSHSIERRSS"
     gene            2037..2780
                     /locus_tag="VNN25_10360"
     CDS             2037..2780
                     /locus_tag="VNN25_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HXH91971.1"
                     /translation="MTTHEVVSQEQWLEARRALLAEEKAFTRERDRLSQKRRALPWVK
                     VAKSYVFRGVEGSLTLRDLFDGRSQLVVYHFMFGPEWQEGCPGCSLLCDQVDGARQHF
                     EHNDVSFVAVSRGPIEKLQAYRERMGWKFRWVSSAAGDFNLDYHVSFPKGTREHGVFY
                     NFDDRPDPEIDELPGVSVFYQEDDGTIYHTYSTYARGGEMFLAVYGWLDVVPKGRNET
                     KNGNLTDWTKRHDRYDNDGRTRSVNGESR"
     gene            2777..3436
                     /locus_tag="VNN25_10365"
     CDS             2777..3436
                     /locus_tag="VNN25_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXH91972.1"
                     /translation="MITLSAFGWVPPFAQGIVRDVRVRWALEEAGIPYRERLIGAEDQ
                     ATAGYRALQPFGQVPAIESGDLKLFESGAIVMHIAERSDALMSAEANQRARTSAWTFA
                     ALNSVEPHIQNLTEIDLFHAKEAWAPMRRPMAVEAVRRRLNALQNWLGDRDYLENRFT
                     AADLMMTTVLRILRHTDMLTEWPALKAYKERCEARPAFKKALADHLKPFEAHLAVSNS
                     S"
     gene            3494..4183
                     /locus_tag="VNN25_10370"
     CDS             3494..4183
                     /locus_tag="VNN25_10370"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HXH91973.1"
                     /translation="MSLSKSLRHVAMVACAAAGLGLTFSSAVLGADPPPDVTPDGLHL
                     RKSTKSTVVYVKPGATFGKYDKVAILECLVEFDKNWQRDYNSSATTRVSDSDMDRIKK
                     GLADEFKKVFTKELTKGGYQVVDSAAPDVLVLRPAIVNLRVTAPDLMTPGISRTVMRS
                     AGSMTLYLELWDSTSNTILARAMDAQSDQGMNGQVSSRVTNTAAASTILRNWADVLRD
                     RLDAARAAPPG"
BASE COUNT          853 a         1374 c         1390 g          844 t
ORIGIN      
        1 gaaggcgctt cacactcgcg tcttcctcgg cattccccgg cgcgggcaac gcaatgtagt
       61 aatggccgcc gggtgcgagg taggggccga gaatgtccgt ccagtaaccg ccgcccggcc
      121 acaattcaac gaccgtcatc gtgggggtaa gtccaaagaa cgtcagttct tcgaccggat
      181 gccgggcgga gtccctggca acggcagcgg ggctgcgcgc ggcactaccg actgcgctta
      241 cgagcgcagg atcagggctc gcggccgctg cgataccgac ggatccggcg atggcgagca
      301 cggtcgccgc aaggagtgcg tggaaatgaa gatctcgcat ggctgtacct caatccggtc
      361 gtgggtggct tcgtagtatc acaaattcac gaacgcgcgt gtcgctgtcg gcactcgtgt
      421 tcttcagttg ggcggcggat tgtctcccgt aaacggtatc ggcgtcgccc ccgtcaccgt
      481 cccgatggtg gccttgtcgc tgacgtagag cttcaccgtg cgctcgaagc gaagttctcc
      541 ctgcacggtg gcaccgggtc caatcacgat ttgaggaacc tcgtgaagga tactcaacat
      601 ctcgccggac ggcttctgca ccaggatgcc gccctccacc cgcgatccgc cggtgatgct
      661 catgctgccg ctgaccgtct tgattccgcc ggccacgtgt gcggagacca gggtgatttt
      721 gccattcacg ttggaaaggg taccgttcac ttccgcgctg tcggacaaat tgagttcgcc
      781 gttgaccgag gaaaccgcac ccgacacgcg cgcaccggcg cccagggtta tggagccgtt
      841 cacgctggtc agtgagtccg ctgtcgcgtg gccgtccaga tgcacgctgc cgttgaccgt
      901 cgaagcgctg gtgatcgtgg cattgtcatc gacgtgaatg gagccgttga ccgttgcgac
      961 cgaccccgac ggcttgccgg cggtcacgtg aatcgatcca ttgatcttgt tcgatccgtc
     1021 gccgtcatct gccccgctgc agcccgcaag cgccgccaac agcgcaacgg cactgcaaaa
     1081 gccgacaaga ccaatgctgc gcatgacgcg gtgttctgtg ctcatgaggt ctccattcga
     1141 aatcaagaat ggcgctactt taccagtacg gctccagacc tcaacttggc gagcggctct
     1201 ttggggttga caagccgcaa tattctggct aaaacttcac tatatggtga actataacac
     1261 gcaactcgac cgaacgtttg cggcgctggt ggacccgacg cgccgcgcaa tcctggcccg
     1321 tctcgaacgc gaggatggcg cctcggtaag cgaactcgcg aaacccttcg ccatcaagct
     1381 tcccgccgtg atgaagcacc tcgacgtgct tgttgaggcc gggctcgtca ctcggtccaa
     1441 gctcgggcgg acggtaaccg tgcggctgtc gccgcaaccc atgcgcgcag cattggattg
     1501 gctgcgtcgc tacgagcgat tctggtcggg cagcctggat cgtttggcgg tatatgccga
     1561 acgcaaggaa gctgaagcga ggaagcgcgg ccgatgacga gccttacctt ggtgcggcgg
     1621 attgctgcac ggccatcgat cgtgtttgag gcgctgacgt cgcctgaagg catcgtgcac
     1681 tggtggggcc cggatgccgg tccggtgctg ttggcggaga ctgatgtacg ggtcggcggg
     1741 cgattccggg tgcgcttccg cactctcgac ggcagcgagc acgaaagcag cggcgagtat
     1801 ctcgagatcg tcaatcccgc gcggctcgcc atgagttggc ggtggaaagg cggcgaggat
     1861 cccggcgaat cgctggtcga aatcgatctt cgaggcattg cggaaggcac cgagctcact
     1921 ttcactcatt cccgtctgcg cgacgagagc acgcgtcgca gccacgagga gggctggaac
     1981 ggagctctcg acaagctgca ataccatttt tcccattcca tcgaaaggag gtcatcatga
     2041 caacgcacga ggtggtctcg caggagcaat ggctcgaagc ccgccgcgcg cttttggccg
     2101 aggagaaagc gttcacccgc gaacgcgacc gcctgtcaca gaagcgccgc gcgctgccct
     2161 gggtcaaagt cgccaagagc tatgtttttc gcggcgtcga ggggtcgctg acgcttcgcg
     2221 atctgttcga cggtcgcagc caactcgtcg tctatcactt catgttcggc cccgaatggc
     2281 aggagggatg cccgggctgc tcgttgcttt gcgatcaggt cgacggcgcc cggcagcatt
     2341 tcgaacacaa cgatgtcagc tttgttgccg tctcgcgcgg tccaatcgag aagctgcaag
     2401 cctaccgcga gcggatgggc tggaagtttc gttgggtttc ctctgccgca ggcgatttca
     2461 atcttgacta ccacgtgtcc ttcccgaagg gcacgcggga gcacggagtt ttctacaatt
     2521 tcgatgatcg ccccgatccc gagatcgacg aactccccgg ggtgagcgtt ttctaccagg
     2581 aggacgatgg gacgatctat cacacgtact cgacttatgc gcgcggtgga gagatgtttc
     2641 ttgcggtcta cggctggctc gacgtggtgc ccaaagggcg caatgagacc aaaaacggca
     2701 accttaccga ctggaccaag cgccacgacc ggtacgacaa cgacggccgg acccgttccg
     2761 tcaacggaga atcccggtga tcacactgag cgcgtttggc tgggttcccc cgttcgccca
     2821 aggaatcgtg cgcgatgttc gtgtgcgctg ggcgctcgag gaggcgggaa tcccctatcg
     2881 agagagactg atcggagccg aggaccaggc caccgccggc taccgcgcac ttcagccctt
     2941 cggccaggtc cccgccatcg agagcggcga tctcaaacta ttcgaatcgg gcgccatcgt
     3001 gatgcacatc gccgagcggt ccgatgccct gatgtcggcg gaggccaacc aacgggcacg
     3061 aaccagcgcc tggactttcg ccgcattgaa ttcggtcgaa ccccacatcc aaaacctcac
     3121 cgaaatcgac ctgtttcatg ccaaggaggc ttgggcgccc atgcgccggc cgatggcggt
     3181 cgaggccgtc cgaagacggc tgaatgcgtt gcagaactgg ctgggggacc gcgattatct
     3241 cgaaaatcga ttcaccgcgg cggatctcat gatgaccacc gtgctgcgga tcctgcgcca
     3301 taccgacatg cttaccgaat ggccggcgct caaggcatac aaggaacgct gcgaggcgcg
     3361 gcccgcgttc aagaaggccc tggcggacca tctcaagccg ttcgaggcgc acctcgcggt
     3421 ttccaattca tcttagggac tgctataaac acttcctgtc gtcgcgctgc ggcctgttgg
     3481 cggggagacg tttgtgagcc tgagcaaatc gctgcgtcac gtagccatgg tcgcctgcgc
     3541 cgcagcaggc ctgggtctga ccttctcgtc cgcggtactg ggtgctgacc cgccgccgga
     3601 tgtcactccg gacggcttgc acctgcgaaa gagcacgaaa tccaccgtgg tctacgttaa
     3661 gccgggtgcc actttcggca agtacgacaa ggtcgccatc ctggaatgcc tggtcgagtt
     3721 cgacaaaaac tggcaacgcg attacaactc gagcgccacc acccgggtca gcgattccga
     3781 catggaccgg atcaagaagg gcctggccga cgagttcaag aaagtattca cgaaggaact
     3841 gaccaagggc ggctaccagg tcgtcgacag cgcggcgccg gacgttctcg ttctgcgccc
     3901 cgccatcgtg aatttgaggg taacggctcc ggacctgatg actcccggca tcagcaggac
     3961 ggtgatgcgt tctgccggtt ccatgacgct gtatctggaa ctctgggatt cgacgagcaa
     4021 caccattctg gcgcgcgcga tggatgcgca aagcgaccag ggcatgaacg gacaagtctc
     4081 cagccgcgtg accaatacgg cagcggctag cacgatcttg aggaattggg cggatgtttt
     4141 gcgcgatcgt ttggatgccg cacgcgccgc gccgcccggc tgatcgctcg tcgcgccgcc
     4201 cggctgatcg ctcgccgcgc ggggcagcaa ctcggagtcg cgcatgctag gattccgtga
     4261 gaactgaata aaaacaccta ggctgccttg acgaccggac cgcacttctt caccgctcca
     4321 actccttcgg cagggcgccg atgaccgaga aagctgtctt tctcagttat gcctcgcagg
     4381 acgccggcgc ggcgctgcgg atctgcgagg ccttgcgcgc cggcggcatc gaggtctggt
     4441 tcgaccagag tgaattacgc g
//
LOCUS       DATLVW010000340         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_592295, whole genome shotgun sequence.
ACCESSION   DATLVW010000340 DATLVW010000000
VERSION     DATLVW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592295"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..908)
                     /locus_tag="VNN25_10375"
     CDS             complement(3..908)
                     /locus_tag="VNN25_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH91974.1"
                     /translation="MQRALAAVLLFTACAAAGAPIGKRVDIGGRSLHIVCMGDGPHTV
                     VMESGAAVGFYEWWLVQNALKNRVRTCSYDRAGFGWSDPPPSRSVAGYISDLHELLRL
                     NGEKPPFILVGHSMGGSFVQRYYWRYPSEVAGIIAVDAANLESSQPKLPEYQQAAAAH
                     RARRIKEMEEWRATDKWPKQQFPSELPPDLRAKLVAASASRNWWEARFGEGSLPDLEV
                     ALTPEQRRIQVPLVVIAAQARNPDGWSDEATERLRKHSLEGQEEIASRSPQSKIVHTT
                     SGHDVPIEAPDVVVNEITIMARGVW"
     gene            complement(1120..1629)
                     /locus_tag="VNN25_10380"
     CDS             complement(1120..1629)
                     /locus_tag="VNN25_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91975.1"
                     /translation="MQRNFISVRIRRACCAVAITWCLTLGSHNALASDAPKVASGLDF
                     VVGTWTGTSTCVGNRPACKNETVVYRFVPLDGHPNQVRLYADKIIDGKRVPMGALVFE
                     VDEHRGTLRGEFTRGQTQGEWSFAVIGKTMTGKLVILPERSVGRDVKARRPTGAEVPA
                     APPVSDYDN"
     gene            complement(1703..3040)
                     /locus_tag="VNN25_10385"
     CDS             complement(1703..3040)
                     /locus_tag="VNN25_10385"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH91976.1"
                     /translation="MTRRSRLVSGTFTALLLIAVVAQAGELPRGGFLGLQVGAVSAEE
                     VRGLGIASGVKVLKLVDGGSGKAAGIQPGDVITQIDSSKVVGTDAFVDFARHLHAGDV
                     VTISLLRGGKPKDIRMEVRPRPFETSPDAGVWYSSISTADGSRRRVIVTKPKQPGRHP
                     AILYMTGIGCFSQESLDRSSPDAKLLYGLTAAGFVTMRVEKSGMGDSEGPPCASPDAD
                     LDNEVRGYMAGLLALKQYDFVDSRNIFLLGLSIGGVEAALVARQEPVRGVVVVNTVAK
                     PFLEYLLETRRRQLQLRHVPYDEIDRRLRENELCNHRLLIEKEPAEALVKRLPSCREH
                     VDYPAPVTFMRQWAAIDPGAEWKEVSAPVLIIYGLSDFVATTGDHPYLAAVLNSFHPG
                     QATLKPIAGMDHYLTRAASMEESITRPAGTSGEFDSDVVDTITSWLRTQRIST"
     gene            complement(3484..>3893)
                     /locus_tag="VNN25_10390"
     CDS             complement(3484..>3893)
                     /locus_tag="VNN25_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91977.1"
                     /translation="TVVADHRHGSPAAFRLKAGESVTADYGFVVTTRPGRIRATSPVT
                     IGEGKDETSLQPGDVVYVLHYVGEGNDLLWFKGREFSEELHTDKPGPIEHWKGLELLE
                     APTTEWWVRMKNSTGKVGWTTRAGDFDGADACE"
BASE COUNT          723 a         1230 c         1180 g          760 t
ORIGIN      
        1 ttctaccaga ccccgcgggc catgatcgtg atctcgttga ccaccacatc cggcgcttcg
       61 atcggtacgt cgtggccgct ggtcgtgtgt acgatcttcg attgcggaga gcgcgaggcg
      121 atctcttcct ggccttcgag cgagtgcttg cgcaagcgtt ccgtcgcttc atccgaccag
      181 ccgtccgggt tgcgcgcttg cgccgcaatc accacgaggg gaacctggat gcgccgctgc
      241 tccggggtca atgccacttc gagatcgggc aatgatccct cgccgaatcg cgcttcccac
      301 cagttgcgcg acgcgctcgc agccacgagc ttcgcgcgca ggtccggggg aagctcggag
      361 gggaattgtt gcttcggcca tttgtcggtg gcgcgccact cctccatctc tttgatccgt
      421 cgcgcccggt gcgcggcggc ggcctgctga tactcgggga gcttcggttg cgacgattcg
      481 agattcgcgg catcgacggc gatgatgccg gcgacctcgg acgggtatcg ccagtagtat
      541 cgctgcacga acgatccacc catcgagtgg ccgaccagga tgaacggcgg tttctcgccg
      601 ttgaggcgca gaagctcgtg gagatcgctg atatagccgg caacactgcg ggacggcggc
      661 ggatcgctcc agccgaagcc ggcgcggtcg taggagcagg tgcgaacgcg attcttcaag
      721 gcgttctgca ccagccacca ttcgtaaaag ccgacggcgg cgccggactc catgacgacc
      781 gtgtgcggtc cgtcgcccat gcagacgatg tgaagacttc gcccgccgat gtctacccgt
      841 ttgccgatgg gtgcacccgc cgcggcacac gccgtgaaaa gaagaaccgc tgcaagtgct
      901 cgttgcatca tttccgttta cgttccggcg atgtcatcgg attggggtcg cttctgtctt
      961 ggattgttga gaacacggtc agcgtgtggc ggtcgccttc acgattggtc gccgaagcaa
     1021 aggcaaccac agagacacag agaacacaga gtgatctctg tgacctctgt gtctccgtgg
     1081 tgaccgattt tcaaattgag ccaagaacgc agccggccat caattgtcat agtcgcttac
     1141 gggcggagcg gcaggcacct ccgcaccggt cggccgccgc gccttcacgt cgcggcccac
     1201 ggatcgttcg ggaaggatga cgagctttcc tgtcattgtc ttcccgatca ccgcgaacga
     1261 ccattcgccc tgcgtctgcc cgcgtgtgaa ttcgccgcgc aacgttccgc gatgctcgtc
     1321 gacctcgaac accagcgctc ccatcggaac gcgctttccg tcgatgatct tgtcggcgta
     1381 gaggcggact tgattcggat gcccgtcgag cggcacgaac cggtagacca ccgtctcgtt
     1441 cttacaagcc ggccgattcc cgacgcacgt cgaggtccct gtccacgtac cgacgacgaa
     1501 gtcgagcccc gacgccactt tcggcgcgtc cgacgccagc gcattatggg atccgagggt
     1561 caggcaccac gtgatggcga cggcgcagca tgcgcgtcgg attcgtacgg agatgaagtt
     1621 ccgttgcatt cgcaaatact cgctcgatcc gtcgcggcag gcaagccgtg agctcttcgt
     1681 tgagctccat ggcctttctt tgtcacgtcg aaatccgttg cgtgcgcagc cacgaagtga
     1741 ttgtgtccac tacgtcggag tcgaactcgc cggacgtccc tgccggtctc gtgatgctct
     1801 cttccatcga ggccgcgcga gtcaagtagt gatccatgcc cgcgatcggc ttcagcgtcg
     1861 cctgccccgg atgaaagctg ttgagaaccg cggcgagata cggatgatcg ccggtggtag
     1921 cgacgaagtc ggagaggccg tagatgatca gaaccggtgc gctgacctcc ttccactccg
     1981 cgcctgggtc gatggcagcc cattgccgca tgaatgttac cggcgcggga tagtccacgt
     2041 gctcgcggca agagggtagc cgcttgacca acgcctccgc cggctctttc tcgatcagca
     2101 agcggtgatt gcaaagctcg ttttcgcgca gccggcggtc gatctcatcg tacggcacat
     2161 gccggagctg cagttgccgc ctccgcgttt caagcaggta ctcgaggaac ggtttggcca
     2221 ccgtgttcac gacgacgacg cctctgacgg gctcctgccg cgcgacgagc gcagcttcga
     2281 cccctccgat gctgagtccg aggagaaaga tgttccggga gtcgacgaag tcgtattgct
     2341 tcagcgcgag caaccccgcc atgtagcctc gcacctcatt gtccagatcg gcgtccggac
     2401 tcgcgcacgg cggcccttcg ctgtcaccca tgccgctctt ttccaccctc attgtgacga
     2461 agcctgccgc cgtcagcccg tagagcagct ttgcatcggg tgagctgcgg tccagcgact
     2521 cctgcgaaaa gcagccgatg cccgtcatgt agaggatggc cggatgcctt ccgggctgct
     2581 tcggtttcgt gacgatgacg cgtcgccgtg aaccatccgc ggttgagatc gacgagtacc
     2641 agacgccggc atcgggcgaa gtctcgaacg ggcgcggcct cacctccatc cggatgtctt
     2701 tcggtttgcc cccgcgaagc agtgagatcg tcacgacgtc gccggcgtgg agatgcctcg
     2761 cgaaatcgac gaacgcgtcg gtgcccacga ctttgctcga gtcgatttgc gtgatgacat
     2821 cgcccggctg aatgccggcc gccttccccg acccgccatc gacgagcttg agcaccttga
     2881 cgccgctcgc gatccccaac ccccgcactt cctcggccga aacggcgccg acctgcagtc
     2941 cgagaaatcc gccccgagga agttcgccgg cttgcgcaac aaccgcgatg agtagaagcg
     3001 cggtgaatgt tccggacaca aggcgagatc gtcgggtcat catggagcat cgtaacaaag
     3061 gcgggatttg gcagcttgcg ggttcgccgc tcggttggcg gtcaccaaaa ccacaacggc
     3121 cgccggacct tgacaaggtg tgtcaccgat gccctcaatg agcgtgcgac gagacgcacg
     3181 cccacgagta ctccagcgac aaagacgccc aataaggcgg caatccctgg atagtcggat
     3241 gccttatcca gcgcgaacag cttgatcaga agcacagtgg caaggccagc gaccaaggtt
     3301 atgaagattg actgaaggcg cgattcaagc gggaaagcag agtatccatg acacttaggg
     3361 catcgaaacg gcagtcttcg aagcccgaat tgaatgcgca tcggctctcc gcagtgcgga
     3421 cagggcagcg tatccggaac gcgtgaaccg gagtccgacg gtgagggagt cgtgatcggc
     3481 tgtttactca caggcatctg cgccgtcgaa gtcgcccgcg cgcgttgtcc aaccgacctt
     3541 gccggtgctg ttcttcatcc gcacccacca ttccgtggtt ggtgcttcga ggagctccag
     3601 ccccttccag tgttcaatcg ggccaggctt gtcagtgtga agctcctctg agaactctct
     3661 acccttgaac cagaggaggt catttccttc cccgacgtag tgcaggacgt agacgacatc
     3721 tccaggttga agagatgtct cgtccttgcc ctcgccgatg gtaactggcg aagtcgcgcg
     3781 gattcgtccc ggacgcgtcg tgacgacaaa tccatagtcg gccgttactg attcacctgc
     3841 tttgagccga aaagctgcag gagagccatg ccgatgatcg gcgacaactg ttg
//
LOCUS       DATLVW010000341         5651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_348374, whole genome shotgun sequence.
ACCESSION   DATLVW010000341 DATLVW010000000
VERSION     DATLVW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348374"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VNN25_10395"
     CDS             <1..1331
                     /locus_tag="VNN25_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91978.1"
                     /translation="AFAASAIAQQCTPDDTLLVCWRKYNSVAAKTAQSVATTNTGTPS
                     ATTQAAATALRDFQSLFSAGLDAGTVVENATSVTLDWNIPFPFVEDNDRIKLQTFFTD
                     PWLAADVSTLLGSNATTAKSQLTNFDDVATLISYSPVNQRFGRSIAPHIDFLRYLETA
                     NSDPAAQLQKIAQLLADVPPTILEGSKFSEITDPAQQAAVIAQVEAAAAAEQKSSTRA
                     TAIVKALTQVINNQPQVYAGGTYHYRNALVGPDEISVKGTFELSPKSLNRFLKSNDSV
                     CDPAKNTTSGKVNATNAATCSSRLISFAGGASQANASDRLAFAIEYKRAKGESVDLTK
                     FMVVPPNTPVVRPETKSLIYSLTYGRPLPNTKIKDARVDVAVNYTNISNDPTMKDRFV
                     ATLTFSQKVSDTMTLPLSFTYANHTQYLPQTDRRVGVHFGVSYKIPNSGQ"
     gene            complement(1526..4153)
                     /locus_tag="VNN25_10400"
     CDS             complement(1526..4153)
                     /locus_tag="VNN25_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXH91979.1"
                     /translation="MTTGSDAHVWFEYAFGGSEPAFKATVQSVSAGTVGESFSDRVET
                     FPCAIFAFRAVARNAYGTTYGSVVSGQAQGCTGDARPGVVAKAATDITSTSVVLHADV
                     DVTGVVGWIGGINVGTAAQFVVGSTADVVEAGLTGVSSPTFVAPSGSTGVYQVVFSTS
                     AACGQEYKFQPLAFGLNGGPVFGSVMSFTTAPCTPSPKWVALGDSYSSGEGTKNYIAG
                     TDVFDANFDPPIVDLCHRSNLAYSRVSRNSPDIADVFLACSGATTLNVLAAPEGKPKC
                     VQEQEFPHLCVFSYAAPDDIPQLTHPEVASADLVTLTVGGNDIHFADVLHWCWTESDC
                     RSYMPFKGDANPTLAAMTLADYVPQLIANERAHVQLTLEAIRAKAGSQADIRIFGYPA
                     LFPSDRSHQVCSDLTVLGKTWSPDEQTWINNLVPTLNGAIREAAFDANARFVSVAEYF
                     SGHEICGSQGSWFVSPTLWAGAFVFNGTSEFFHPTAEGQREGYRRALEDDFAKVPRGS
                     HAKSAVPNEVQRALMRRKVADDSASLPTLDQLNFATIAPLCPRVVVAGQQLSLSGDGF
                     APGATVTITLNASDPLVLGMVTADLSGQFGTTITIPADVVPTPLARLEAAGKGANLVS
                     RLLLAYPTVGPAATVDQDGDGIPDACDNCPATSNVSQTDTDGDGIGDACDPCPDDPLN
                     RCTAALFGPPYGFSATATSSSQVALMWAAVPGAESYEIYRRDSGSVFSLLKATTGTAA
                     TDSGLMPETTYLYQVRAIGASGPSGFSAVDPATTIVFADDPLLAGTTTKAIHVTQLRT
                     AVNSMRAAAGLPAASFTSTLSVGAGINAVDLTELRSALDQARSAIGLYSITYTDPTIT
                     PAVTPLRTSHITDLRGGVR"
     gene            complement(4182..4448)
                     /locus_tag="VNN25_10405"
     CDS             complement(4182..4448)
                     /locus_tag="VNN25_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91980.1"
                     /translation="MTQFFRFERHAAHGLEVQEALLSGDDANMMDVIHGDEDSFRHRE
                     IADPSFVLEPRRDVSFNRGSARAREGKLRPARSSGRLYRRRPLI"
     gene            complement(4464..>5651)
                     /locus_tag="VNN25_10410"
     CDS             complement(4464..>5651)
                     /locus_tag="VNN25_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXH91981.1"
                     /translation="RQTNPAKHLEVHVTPEMIRHSRIYDVLYFTDVVYGIGILLLILA
                     TGWSGRMRNLSAKYARWSFVAAMLYFVLLSLVTTALTFPLDFYGSFVVPHQFDLTNQT
                     FAGWFGDFAKGLGVNLVIGAPIAALALLGIRCIRRWWIVLWLGSIPLILLGVLATPLI
                     IDPLFNDFRPLRDASLRQALLDEASHAGIEQSRVYEVDKSKQTKEMNAYVTGIGPSAR
                     IVMWDTLLAKLDRDEILAVMGHEMGHYVLKHLWWGMAFGVAISFLGFWIAQYLFEWGL
                     ARWGVRWSAGTRGDPAALPWLLAVSSLLAFLLSPVYSGYSRHVEHQADIFGLELTHKN
                     EAMASSFVKFAEDSKADPRPPRFIEWWRYSHPSLGRRIDFVLAYKPWEKGQPNELWHP
                     AGK"
BASE COUNT         1243 a         1738 c         1660 g         1010 t
ORIGIN      
        1 tggccttcgc cgcaagcgcg attgcccagc aatgcactcc cgacgacacg ctcctcgtct
       61 gctggcggaa gtacaacagc gtggccgcaa agaccgcgca gagtgtggcg acgacgaata
      121 caggaacgcc gtccgcgacc acgcaggcgg cagcgactgc cctgcgcgac ttccagagct
      181 tgttctccgc ggggctcgac gctggcaccg tcgtcgagaa cgccacctcg gtcacactcg
      241 actggaacat ccccttcccc ttcgtcgagg acaacgaccg catcaagctg cagacgttct
      301 tcacggaccc gtggctcgca gccgatgttt ctacgctcct cggatcgaac gccaccaccg
      361 cgaagtccca attgacgaac ttcgacgatg tggccacgct catctcgtac tcgccggtca
      421 accagcgctt cggacgctcg atcgcaccgc acatcgactt cctccgttac ctggaaacgg
      481 cgaattccga tcccgccgcg caactgcaga agatcgcgca actcctggcc gacgtgccgc
      541 cgacgatcct ggagggctcg aagttttcgg agattacgga ccccgcgcag caggcggcgg
      601 tgatcgctca ggttgaggca gccgcagcag cggagcagaa gtcttcgacg cgcgcgaccg
      661 cgatcgtgaa ggcgctgacg caagtgatca acaatcagcc gcaggtctac gccggaggta
      721 cgtaccacta ccgcaacgcg ctcgtcggcc ccgacgagat ctcggtgaaa ggaacgtttg
      781 agctctcgcc gaaaagcctc aaccgattcc tgaagagcaa tgacagcgtc tgcgatcccg
      841 cgaagaacac gacgtcgggc aaagtgaacg ccacgaacgc ggcgacctgc tcgagccgcc
      901 tgatctcgtt cgccggcggc gcttcacagg ccaatgccag cgatcgcctc gccttcgcca
      961 tcgagtacaa gcgggcaaag ggggagagcg tcgatctgac caagttcatg gtcgtgccgc
     1021 ccaacactcc ggtcgtgcgc cccgaaacaa aaagcctcat ctactcgctc acgtacgggc
     1081 gtccgctccc gaatacgaag atcaaggatg cgcgcgtcga cgtcgccgtc aactacacga
     1141 acatttccaa cgatcccacg atgaaggacc gtttcgtcgc caccctgacg ttctcgcaga
     1201 aagtcagcga cacgatgacc ttgccgctct ccttcaccta cgccaaccac acccaatacc
     1261 tgccccaaac cgatcgccgc gtcggcgttc attttggcgt gtcgtacaag atcccgaata
     1321 gcgggcagta gagaatcgta acgaggcaga aaaggtcacc aatgtctgac tcatctatgt
     1381 cgttcgacca ctcgcagctc gcccgcacct acgcaccggc agaatctcgt tcattttcag
     1441 acctgtggca cctggcgcaa ccctcgatca cggcctgttt atatgtcgct ttgagcgacc
     1501 cgcattctgg cgatctcggc ctcaactacc tcacgccacc acgaaggtcc gttatgtgag
     1561 acgtcctaag cggagtcacg gccggcgtga tcgtcggatc agtataggtg atcgagtaaa
     1621 ggccgatcgc ggaacgggcc tgatccagcg ccgatcgaag ctctgtcaag tccacggcgt
     1681 tgatccccgc cccgacactc agggttgagg taaagctcgc cgcggggagg cctgccgccg
     1741 cgcgcatgga gttgacagcg gtacgaagct gagtgacgtg aatggctttc gtggtcgtgc
     1801 cagcgagaag gggatcgtca gcgaacacga tggtcgtcgc gggatcgact gcgctgaaac
     1861 cggacggccc ggaagcacca atcgctctga cttggtaaag gtacgtagtc tccggcatga
     1921 ggccggagtc agtggctgcc gtccccgtcg tcgccttcag gagtgagaag acactgcctg
     1981 aatcacgtcg atagatctcg tacgactcgg cgcccggcac ggcagcccac atcaacgcga
     2041 cttgagaaga gctcgtcgcg gtagcgctga acccgtaagg tgggccaaac agcgctgccg
     2101 tacagcggtt cagcggatca tcggggcagg gatcgcaggc atcaccgatt ccatcgccat
     2161 cagtgtccgt ttgcgagacg ttggaggtcg ccggacaatt gtcgcaagcg tccgggatgc
     2221 catcgccatc ctgatcgacg gtcgcggctg gcccgaccgt gggatatgcg agaagcaagc
     2281 gcgagacaag attcgccccc tttcccgcag cctccaaacg cgcaagcggc gtcggcacta
     2341 catcagcggg aatcgtgatc gtggttccga attgaccact gaggtccgca gtcaccatgc
     2401 ccaagaccag tggatcggat gcattcaacg tgatcgtcac ggtcgcaccg ggcgcaaaac
     2461 cgtcaccgga aagcgagagc tgttgtccgg cgacgaccac tcttggacaa agaggagcga
     2521 tcgtggcgaa attgagttga tcaagcgttg gcagggacgc ggaatcgtca gcgaccttcc
     2581 gccgcatgag ggcgcgttgg acttcattcg gcactgcact cttggcatgg ctaccgcgag
     2641 gaacctttgc aaaatcgtct tcaagtgcac ggcgataacc ctctcgttga ccctcagccg
     2701 tcgggtggaa aaactcagac gtcccgttga acacaaacgc ccctgcccaa agcgtcggtg
     2761 aaacgaacca cgagccctgt gaaccgcaga tctcatgacc agaaaagtat tccgcgacac
     2821 tcacaaagcg cgcgtttgca tcgaacgccg cctcgcggat tgcaccattg agcgtcggaa
     2881 cgaggttatt aatccatgtc tgctcgtccg ggctccatgt ctttccgaga acggtcaaat
     2941 ctgagcacac ttggtgggac cggtcactgg gaaacagagc cggataccca aaaatacgaa
     3001 tgtctgcctg ggaaccggcc tttgctcgaa tcgcttcgag agtcagctgc acgtgagctc
     3061 gctcgttcgc gatcagttgc ggaacgtagt ccgcaagtgt catcgcggcc agtgtcgggt
     3121 tcgcgtcgcc cttgaagggc atgtagcttc gacaatcgct ttcggtccaa caccagtgca
     3181 gaacgtcggc aaagtgtatg tcgttaccgc cgactgtaag cgtcacgaga tccgcggaag
     3241 ctacttccgg gtgagtaagc tgagggatat cgtcaggggc cgcataggaa aacacacaga
     3301 gatgcgggaa ctcttgctcc tgaacacatt tcggtttccc ctccggtgcg gccaggacgt
     3361 ttagtgtcgt cgcaccgctg caagccagga aaacatccgc gatatccggc gagttccggc
     3421 tcactcttga ataggccagg ttcgagcgat ggcacaagtc gacaatgggg gggtcgaagt
     3481 tcgcgtcgaa gacatccgtc cccgcaatgt aattctttgt gccctctccc gacgaatagg
     3541 agtcgccaag tgcgacccac tttgggctcg gcgtgcaagg agcggtcgtg aacgacatca
     3601 ccgaaccaaa taccggcccc ccgtttaggc cgaatgccaa gggttgaaac ttgtattcct
     3661 gaccacaggc agctgaagta ctaaaaacga cttgatatac accagtcgaa ccagacggag
     3721 cgacaaacgt cggtgaactg actcctgtga gtcccgcttc gacgacatcg gcagtcgagc
     3781 cgacgacgaa ctgcgctgcc gttccgacgt ttatgccgcc gatccagccc accacacctg
     3841 tcacatctac atcagcatga agaacgacgc tcgtcgacgt gatgtctgtg gcagccttcg
     3901 caacaacgcc gggcctcgcg tcacctgtac atccttgcgc ctggccggag acgaccgaac
     3961 catacgttgt cccgtatgca ttgcgggcta ctgctcgaaa agcgaaaatg gcgcatggga
     4021 acgtctccac cctgtcggag aacgactcgc cgaccgtgcc ggcgctcaca gactgcaccg
     4081 ttgccttgaa cgctggttca gaaccgccaa aagcgtactc aaaccagacg tgcgcatcgg
     4141 accccgtcgt cactgtgcca ttgagcgcaa cgaagggata atcaaatgag ggggcggcgc
     4201 cggtagagac gaccggagga gcgtgcgggt ctgagtttcc cctcacgggc tcgcgcgctg
     4261 ccgcgattga aggagacatc acgacgcggc tcaagcacaa agctgggatc agcaatctcc
     4321 cgatgccgga agctgtcctc gtcgccatgg attacatcca tcatattggc gtcatcccca
     4381 gagagtaaag cctcctgcac ttcgagacca tgagcagcat gcctttcaaa acgaaagaac
     4441 tgcgtcatgc accccgcaca tcctcacttc cctgccggat gccagagctc gttgggctgg
     4501 cctttctccc atggtttgta tgcgagtacg aagtcgatcc ggcggccgag ggatgggtgg
     4561 gagtagcgcc accattcgat gaagcgtggt gggcggggat cggctttcga atcctcggcg
     4621 aacttcacga aggagctggc catcgcttcg ttcttgtgcg tcagctcgag tccgaaaatg
     4681 tcggcttgat gctcgacgtg gcgtgagtag ccggagtaga cgggcgacag gaggaacgct
     4741 agcaggctgc tgacggcgag cagccacgga agcgcggccg gatcgccgcg agtgcccgca
     4801 ctccagcgaa cgccccagcg cgcgagaccc cattcgaaga gatattgcgc gatccagaat
     4861 ccgagaaagg agatcgcgac gccgaaggcc attccccacc atagatgctt gagaacgtag
     4921 tggcccatct cgtggcccat cacggccagg atctcgtcgc gatcgagctt cgccagcagc
     4981 gtgtcccaca tgacgattcg cgccgaggga ccgataccgg tcacgtaggc gttcatttct
     5041 ttcgtctgct tcgacttgtc gacttcgtaa acgcggctct gctcgatgcc ggcgtgcgag
     5101 gcttcgtcga gcaacgcttg ccgcaatgaa gcatcgcgca gcggacgaaa atcattgaag
     5161 agcggatcga tgatcagcgg cgtggccagg acgccgagga ggatcagcgg aatcgatccg
     5221 agccagagca cgatccacca ccggcggatg caacggatgc cgaggagcgc cagcgcggcg
     5281 atcggcgccc cgatgacgag attcacgccg agtcctttgg cgaaatcgcc gaaccatccg
     5341 gcgaaggtct ggttcgtgag atcgaactgg tggggcacga cgaagctgcc gtagaagtcg
     5401 agcggaaagg taagcgccgt ggtgacgagc gacaacagca cgaagtacaa catcgccgcg
     5461 acgaatgacc agcgcgcgta cttcgctgag aggttgcgca tgcgccccga ccagccggtg
     5521 gcaaggatga gcaagaggat gccgatgccg tagacgacgt cggtgaagta gagaacgtcg
     5581 tagatccgac tgtggcggat catttcgggg gtgacatgaa cctcgagatg tttcgcgggg
     5641 ttcgtttgtc g
//
LOCUS       DATLVW010000342         5821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_424777, whole genome shotgun sequence.
ACCESSION   DATLVW010000342 DATLVW010000000
VERSION     DATLVW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424777"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VNN25_10415"
     CDS             <1..181
                     /locus_tag="VNN25_10415"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXH91982.1"
                     /translation="HFNLDWHDHVDIDPSLFRASEITHSRGNPEKARGVLGWEAKTKF
                     GALVTLLTNAVRVSV"
     gene            complement(599..1555)
                     /locus_tag="VNN25_10420"
     CDS             complement(599..1555)
                     /locus_tag="VNN25_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HXH91983.1"
                     /translation="MRSKRWIDMRNRNERVTGDGRGVRCEWEFTSDLHVAAQFPSAAA
                     RLMRKAFAQWPIAMRESPVSRAATPAVSFVIGHRGLARLPHLLTTLRSIAGQTDVQVE
                     CIVVEQSSAPEIASRLPSWVHYVHTPTPRPDYEYNRAWTLNQGVREARGDVVILHDND
                     ILVPAAYASECASRVGEGFDFLELKRFTFYLDEPQTKRVFTTGLVPHDVASTVVQNLL
                     GASIAARRSSYFDAGGFDEGFVGWGGEDNEFWERAEVTGKTYRFGYLPFLHLFHQPQP
                     GKMQGSNAPAVRRYAELRIVPAAERIRRLRDERTGVAAEFEE"
     gene            complement(1664..2593)
                     /locus_tag="VNN25_10425"
     CDS             complement(1664..2593)
                     /locus_tag="VNN25_10425"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXH91984.1"
                     /translation="MARRVMLTGAGGFLAGHFATAFAAAGWQTFGIGRSDRDNQAGLF
                     SELTLADLSDLGAITSFAGRVAPDVLVHLAAPASVAESIDDPLADLQAHIIPTANVLY
                     ALRRSSPATRVLLISSAAVYGSPASLPISEDTRLAPISPYGFHKLQQELLFDQYAALH
                     GLRTCKARIFSTYGERLRRLAVWEIARRALAGNPEVFGTGEESRDYLDVTEAARAVVC
                     IADRAPFEGEAINVASGADVSVATVAAEIFRLVGLSEAPRFTGKTLPGNPIRWRADVT
                     RLRKLGCSPASWPSGLARTVAWIAAEYRQASDV"
     gene            2658..3476
                     /locus_tag="VNN25_10430"
     CDS             2658..3476
                     /locus_tag="VNN25_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019489310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH91985.1"
                     /translation="MHKVLRPRHGLARLKLAEVWEYRELMFFLAWRDILVRYKQTEIG
                     ILWAFLRPFITMVIFTVVFGRLAKLPSANLPYPVFALAGLLPWQLFSTAFSEAASSIV
                     GSGQLVSKVYFPRLIIPISATFSVLVDFLVSSLMLAVLMIWYGVRVSANIVWLVPLTA
                     LCMLAAIGSGILFAALYVRYRDIRHILPFIVQFGLYISPVAFMSTMIPAKWRLLYSLN
                     PMVGVIDGFRWALFGGTNVIDMPALALSVALTVVMLIGGLYYFRNTERLFADII"
     gene            3480..4712
                     /locus_tag="VNN25_10435"
     CDS             3480..4712
                     /locus_tag="VNN25_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91986.1"
                     /translation="MSSVLISASGVGKQYFLGADPFGTLSLRDRITDVVRSAFRSRQA
                     AVSRDFWALRDVGFELQRGEVLGVIGRNGAGKSTLLKILSQITEPTEGEVNLYGRISS
                     LLEVGVGFHPELSGRENVFLNGAILGMTRAEMRRKFDEIVAFAEVEAFIDTPVKRYSS
                     GMYVRLAFAVAAHLEPDVLIVDEVLAVGDAAFQRKCLGKMESVASQDRAVMVVSHNMS
                     IVTQLCERVIWLDRGRIVAAGETREVVGAYLTQGSGGADSWEPPALQRAPLQLHTVSI
                     ERPGGGGGDIAADQPFDIAFDYSIVAPISPGRFAIAIKAADATVVLASASTDHLSALR
                     EPWKPGRRCARCRIPGHLLAPGRYFVTVAEPSEDEGDVIHEGVLSVTVSDQNSLVARD
                     GRPGIIAPLLQWSDEELP"
     gene            4709..5356
                     /locus_tag="VNN25_10440"
     CDS             4709..5356
                     /locus_tag="VNN25_10440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXH91987.1"
                     /translation="MIWRLLDPLLLKIARRLEHLAIHHPSDYREQFIRSLGHFSPRAI
                     VTNRARVQSVAPSDHLEVGDYTYIEGEVFLLTPESRCIVGSHSFLGAESRLWVQGTMT
                     IGDFVLIAPRVDIFDNDSHSLDLHDRREDARDLFERNRLMDYSRVPQSGVVIEDDVWI
                     GTKSTILKGVRIERGAIIAAGSVVTRNVPAFTLMAGNPAREIRLLDQRLRSNGQE"
     gene            complement(5326..>5821)
                     /locus_tag="VNN25_10445"
     CDS             complement(5326..>5821)
                     /locus_tag="VNN25_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91988.1"
                     /translation="SMRATTRADRALLETAARAMARGQNVVVVEDEDFERSFWMRAEL
                     VGVDPRWPFLSYVAQQYATHQPVVWPPPPPGGPVNLTGLVKPGGRLLMNAGAGLLIPA
                     NPMHVPSYSVASVIDFREDDALFVPLWTFRNLARRVNPKFRYVYDLGEVEWPGYSWPL
                     LRRR"
BASE COUNT         1092 a         1819 c         1778 g         1132 t
ORIGIN      
        1 ccacttcaat ctcgactggc acgaccatgt cgacatcgac ccatccctct tccgcgcctc
       61 cgagatcacc cacagccgcg gcaatcccga aaaagcgcgc ggcgtgctgg ggtgggaggc
      121 taagacgaaa tttggagcgc tggtcacgct actgacgaac gccgtccgcg tttcggtttg
      181 accatcacac gctgatgcac tgaacgccga tgcgttcttg atcagttgct cgagactacc
      241 gctgtctgag tctcatctcc ccggcttgtc gatagtgtaa tcgtcagctc tcgatggtct
      301 gtgatctgaa cgaaatcgac cttgagtccg agcttcgcaa tcatttccga aaggaactgc
      361 tcgacgtctg ctgtatttgc gggcgcccta tgaaccagcg ctgccaggag ctcgttaatc
      421 tggccgggct ttgtctcgac agggccttga cagttcactc tggagacatt gcttcttgaa
      481 gagttgaggt tggcagtcat ctggaaaagc tccttccttc gcaagataac gcgataacgc
      541 acgaaaagga tacgagtctg agcgcccaaa acgagccttg gatcgaccgt tcccgcgatc
      601 actcctcgaa ttcggcggcc actcctgttc gctcatcccg aagccttctg attcgttctg
      661 ccgcgggaac tatccgcagc tcggcatagc ggcgaacagc gggagcattg gaaccctgca
      721 tcttcccggg ttgtggttgg tgaaacaggt gaaggaaagg caggtatccg aaacggtacg
      781 ttttccccgt aacttcggcg cgctcccaga attcgttgtc ttctccaccc cagccgacga
      841 agccctcgtc gaatccccca gcgtcgaagt aggaagagcg ccgcgcggcg atgctcgctc
      901 ccaggaggtt ctgcacgacc gtcgacgcca catcgtgcgg aacaagacct gtcgtgaaga
      961 cgcgcttcgt ttgaggctcg tcgagataga aagtgaaccg cttcagctcg agaaagtcga
     1021 agccttcacc cacgcgcgat gcacactcgg aagcataggc cgcgggaacc agaatgtcgt
     1081 tgtcgtggag aatgaccacg tcgccacgcg cctcgcggac accctgattg agcgtccatg
     1141 cgcggttgta ttcgtagtcg ggccgcggcg tcggcgtgtg gacatagtga acccacgagg
     1201 gaagtctcga tgcgatctcc ggtgcagagg actgctcgac gacgatgcac tcgacttgta
     1261 cgtcggtctg accggcaatg ctccgcagcg tcgtcagcag atgaggcaat ctggccagac
     1321 cgcgatgtcc gatcacgaag gagaccgccg gagtggccgc gcggctaacc ggtgactcac
     1381 gcattgcgat cggccattgc gcaaaggcct ttcgcatcag ccgcgcagca gcagaaggaa
     1441 actgcgcagc gacgtgaaga tccgacgtga actcccactc gcagcgaaca ccgcggccgt
     1501 cgccggtcac ccgctcgttg cgattgcgca tgtcgatcca gcgcttcgat cgcatggaca
     1561 gcgcgaatcg aggcaggtca tacagtacgg ccccggcgcg cgatcggaat gacatcggca
     1621 tgatggtaat gcagcacgct cggaggccgg tccgccgatt tcttcaaacg tcggaggctt
     1681 ggcggtactc cgccgcaatc catgcgaccg ttcgcgcgag gccggacggc caggacgccg
     1741 gtgagcatcc gagcttgcgt agccgggtca cgtctgcgcg ccagcggatc ggattcccag
     1801 ggagagtctt gccggtgaac cgcggcgctt ccgatagtcc aacgagccgg aagatctcgg
     1861 cggctacggt cgcgacggag acgtcggcgc cggatgcgac attgatcgcc tccccttcga
     1921 acggtgcacg atcggcgatg cagacgacgg cacgtgcggc ctcagtaacg tcgagatagt
     1981 cgcggctttc ctcgccggtg ccgaagactt ccgggttgcc tgctagcgcg cggcgcgcaa
     2041 tctcccacac cgcaaggcgg cgtaatcgct caccgtaggt cgagaaaatc cgagccttgc
     2101 acgtgcgtaa gccgtgcagc gcggcatatt gatcgaagag cagttcctgc tgcagtttgt
     2161 gaaagccata cggggaaatc ggcgccagcc gtgtgtcttc cgaaatcggc agcgacgcgg
     2221 gactgccata gaccgctgcg gacgagatga ggagaacccg cgtagccggt gacgaccggc
     2281 gcaacgcgta aaggacattc gcagtaggaa tgatgtgggc ctgaagatcg gcgagcggat
     2341 catcgatcga ttcggcaacg ctggcaggag cggcgagatg cacgagaacg tccggcgcga
     2401 ctctgccggc gaaggaagtg atcgcaccga ggtccgagag atccgcgagt gtgagctcgg
     2461 agaagaggcc ggcctggttg tcgcggtcgg agcgtccgat cccgaaagtc tgccatccgg
     2521 cggcggcgaa cgccgtggcg aagtgacccg ccagaaagcc gcctgcaccg gtgagcatca
     2581 cgcgtcgcgc catcgccgca gtttatgacc gtttcagctc cccagcctcc tcgggtacgc
     2641 atgctactct cccttccatg cacaaagttc tgcgcccgcg gcacgggctg gcgcggctca
     2701 aacttgccga ggtgtgggag taccgcgagc tgatgttttt cctcgcctgg cgcgacattc
     2761 tcgttcgata caagcagacc gagatcggca tcctctgggc cttcctccgt cccttcatca
     2821 cgatggtcat cttcaccgtc gtcttcggcc gcctggcaaa gctcccctcg gcaaatctcc
     2881 cctacccggt cttcgcgctg gcgggacttc ttccatggca actcttttcc accgcgttct
     2941 cggaggccgc aagctccatc gtaggaagcg gacaactggt atcgaaggtc tacttccccc
     3001 gcctcatcat tccgatcagc gcgacgttca gtgtcctggt cgactttctc gtctcttccc
     3061 tgatgctggc cgtgctcatg atctggtacg gcgtccgcgt cagcgcgaac atcgtctggc
     3121 tcgttcccct gacagcgctg tgcatgctcg ccgcaatcgg gagcgggatc ctcttcgcag
     3181 cgctctacgt gcgctaccgc gacattcggc acatcctgcc gttcatcgtg cagttcgggc
     3241 tctacatctc gcctgtcgct ttcatgagca cgatgattcc ggcaaagtgg aggcttctct
     3301 actccctcaa tccgatggtg ggcgtgatcg acggctttcg ctgggcattg tttggtggaa
     3361 cgaatgtcat tgacatgccg gcactcgcgc tctcggtggc tctgaccgtg gtgatgctga
     3421 tcggcggcct gtactacttc cgcaataccg agcgcctctt cgccgatatc atctgagcga
     3481 tgtcctccgt cctgatctct gcgagtggag tgggaaaaca atatttcctg ggcgccgatc
     3541 cgttcgggac gctttctctg cgcgaccgga ttaccgacgt cgtgcgatcc gcgttccgtt
     3601 ctcgtcaggc agccgtaagc cgcgatttct gggcccttcg ggacgtcgga ttcgagctcc
     3661 agcggggaga ggttctcggc gtgatcggcc gcaacggggc cggcaaatcg accctgctca
     3721 agatcctcag ccagatcacg gagccgaccg aaggtgaggt gaatctctac gggcgcatct
     3781 ccagtctgct ggaggtcggc gttggattcc atcccgagct ctcgggccgc gagaacgtct
     3841 tcctcaatgg agccatcctc ggcatgacac gcgcggagat gcggcgcaaa ttcgacgaga
     3901 tcgtcgcctt cgcggaggtg gaagcgttca tcgacacgcc ggtcaagcga tactcgtcag
     3961 ggatgtacgt ccgtctggcg tttgcggtgg ccgcccacct ggaaccggac gtgctgatcg
     4021 tcgacgaagt actggccgtc ggtgacgccg cgttccagcg gaagtgcctc ggcaagatgg
     4081 aaagcgttgc cagccaggac cgcgccgtca tggtcgtcag tcataacatg agcatcgtca
     4141 cgcaactctg cgaacgcgtc atctggctcg atcggggaag gatcgttgcg gccggagaga
     4201 cccgcgaggt cgtcggcgca tacctgaccc agggcagcgg cggcgccgac tcgtgggaac
     4261 cgcctgcgct ccaacgtgcg ccgctacaac tgcacactgt gtccatcgag cgccccggcg
     4321 gaggtggtgg cgatattgcc gccgaccagc cgttcgacat cgcattcgat tactcgattg
     4381 ttgcacctat ctcgccgggc cgtttcgcaa ttgcaatcaa agcggccgac gccaccgtgg
     4441 tgctcgcatc ggcgagcacg gaccatcttt ccgcgttgcg agaaccatgg aaaccgggac
     4501 gtcgctgcgc gcgatgccgc attccagggc accttctcgc tcctgggcgc tacttcgtga
     4561 cggtcgcgga gccaagcgag gacgaaggag atgtcatcca tgaaggcgta ctctcagtga
     4621 cggtgtccga tcagaacagt ctggttgctc gtgacggccg ccccggcatc atcgctcctc
     4681 tgctgcagtg gagtgatgag gagctgccat gatctggaga ctgctcgacc cgttgctgct
     4741 caagatcgcg cgccggctgg agcacctcgc catccatcat ccgtcggact atcgcgagca
     4801 attcatccgg tcgcttggtc acttttcacc gcgggcgatt gttacgaacc gcgctcgagt
     4861 ccaaagcgtc gcgccttctg atcatctcga agtcggagac tacacctaca tcgagggcga
     4921 ggtcttcctg ctgactccgg agtcccgttg catcgtcggc agtcactcct tcctcggcgc
     4981 cgagtcgcgg ctgtgggttc aagggacgat gaccatcgga gatttcgtgc tgatcgcacc
     5041 ccgagtcgat atcttcgaca acgactcgca ttcgctcgac ctccatgatc gccgcgagga
     5101 cgcgcgcgac ctgttcgagc gcaaccggct gatggactat tcacgcgtgc ctcagagcgg
     5161 cgtcgtgatc gaggatgatg tgtggattgg aacgaagagc acgatcctga agggcgttcg
     5221 catcgaacgg ggggccatca ttgccgcagg ctccgtcgtg acacgcaacg ttcctgcgtt
     5281 cacgctcatg gccggaaacc ctgcccgcga aattcgcttg ctcgatcagc gcctgcgcag
     5341 taacggccag gagtagcctg gccactccac ttcgcccagg tcgtagacgt agcggaactt
     5401 cggattgacg cgccgtgcca ggttgcgaaa ggtccaaagc ggaacaaaga gcgcatcgtc
     5461 ttcgcgaaag tcgatcacgc tcgcgacgga gtaggacggc acatgcatcg ggttcgccgg
     5521 gatgaggagg ccggcgccgg cgttcatcag caaccgtccg cccggtttca cgagtcctgt
     5581 gaggttcacc gggccacccg gggggggcgg aggccagacg actggctgat gcgtcgcgta
     5641 ctgctgcgcg acgtaggaga gaaagggcca ccgcggatcg acgccgacca attctgcgcg
     5701 catccagaat gagcgctcaa agtcctcgtc ctcgacaacg acgacattct gcccgcgagc
     5761 catcgcccgc gctgcggtct cgagtagcgc gcgatcggca cgtgtcgtgg cgcgcatgga
     5821 g
//
LOCUS       DATLVW010000343         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_303824, whole genome shotgun sequence.
ACCESSION   DATLVW010000343 DATLVW010000000
VERSION     DATLVW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303824"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2262)
                     /locus_tag="VNN25_10450"
     CDS             complement(<1..2262)
                     /locus_tag="VNN25_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH91989.1"
                     /translation="MKKRLTIPLAAVAVVVVLAAVAIIVMRQSAARHRVFRAAPEPRL
                     ASTSSEQRSASALTAQFRDLEVNGRWSELARLLARIEKSDPNEYARWSLAYLHARALI
                     ENNEPADAAAKLAPFLAAGNPWRALALYHQAEIDDDRGDHAAASRDRQTLIFDAAASP
                     YRDQAIDDEIEHLARVGNLRALSDFAARLAPTASASRRRDLSARIVELDFRAGDANGA
                     LARGLALLQGGTLDDASDRASRALDRPELIARMNGPQLDLMGQSMQNHRHFDRAVALL
                     SAALKAMPQKRDDLTFDIGRSYFGNEQFAQAQQLYLRGAITAATPLSKASFLWHAARA
                     AQLQSDDRTAEQLMTQVLAVPGRFPPTAAAITQRIRTRLKQHRFAEAGADLAFVRKNW
                     PKDHALVEASLAYAIGMLGAGNNGAAIVTLNAIPRNLTDKLEPYEIDYWRARALESSN
                     PPAAFQAYLNVLRATEPTHFAYFARQRLDAPSMQPKLQRELEIRDAEIARLVAAGNWL
                     AAKQVATDRILLSSTNHAAELKQLADIYRHLPEYANILDLRPEPFPQFPPKAGADRSS
                     LLMAMGLFDETVESIEQRYPLRPPQSALTRSLALNHGNASKESIFAIEVLMNSVPRDY
                     VPELLPLTVRQLLYPRYFYDFIRDDSRSYNADPTLVLSIMREESRFNPRAKSEAAARG
                     LLQFIITTARQIGRDAGLVNVEPEDLYDPRIIIRLGAKYVSTLSKDFNDDHYAVAASY
                     NAGPKQTALWLRMS"
     gene            complement(2259..2708)
                     /locus_tag="VNN25_10455"
     CDS             complement(2259..2708)
                     /locus_tag="VNN25_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91990.1"
                     /translation="MSRILKFDRTPVARLISCAASFASAALVFDYLSKGEKEIEALPM
                     AGLILLFVGTLAAAVLQYGDHIYLSDDGLMYENWVLKHFGRRGRWMRWEDVVEIREIK
                     QKVLILFSRDGRRMLVDAILGYAIAKHEILRRAPGAVFSGTLARDDS"
BASE COUNT          504 a          794 c          926 g          526 t
ORIGIN      
        1 ggacatgcgc agccagagcg cggtttgttt cgggccggcg ttgtaagagg cggcgacggc
       61 gtagtgatcg tcgttgaaat ccttggagag cgtgctgacg tacttggcgc cgaggcggat
      121 gatgatgcgc gggtcgtaga ggtcctcggg ctcgacgttg acgagaccgg cgtcgcggcc
      181 gatctggcgg gcagtcgtga tgatgaattg cagcagcccg cgcgcggcag cttcggattt
      241 cgcgcgcgga ttgaagcgcg attcttcgcg catgatggat agcacgagcg tcggatcggc
      301 gttgtagctc ctggagtcat cgcggatgaa atcgtagaaa tagcgcggat acagaagctg
      361 gcgaacggtg agcggcagaa gctcgggcac gtagtcgcgc ggaacggaat tcatcagcac
      421 ttcaatcgcg aagatcgatt ctttcgatgc gttgccgtga ttgagtgcaa gcgagcgcgt
      481 gagtgcagac tgcggcggcc gaagcggata gcgctgttcg atcgactcca ccgtctcgtc
      541 gaagaggccc atggccatga gtagtgagga gcggtccgcg ccggcttttg gcggaaactg
      601 tggaaacggc tccggcctta gatcgaggat gttcgcgtat tcgggaagat gccggtaaat
      661 gtcggcgagc tgtttcagct cggcggcgtg gttggtcgac gagaggagaa tgcgatcggt
      721 ggccacctgt ttcgccgcaa gccagttgcc ggcggccacg agccgcgcga tctccgcgtc
      781 tcggatttcc agctcgcgct gtagtttcgg ctgcattgac ggcgcatcga ggcgttgccg
      841 cgcgaagtag gcaaagtggg ttggctccgt cgcgcgaagc acgttgagat aggcctgaaa
      901 cgccgcgggc ggattggacg actccagcgc tcgcgcgcgc caatagtcga tctcgtacgg
      961 ctcgagtttg tcggtcagat tgcgcggaat cgcattaagc gtaacgattg ccgcgccgtt
     1021 gttgccggcg ccgagcatcc caatggcata ggcgagcgag gcctcgacaa gcgcgtgatc
     1081 tttcggccag ttctttcgca cgaatgccag atcggccccg gcctcggcga aacgatgctg
     1141 cttcagccgc gtgcggatgc gctgggtgat tgcggccgcg gtgggtggaa agcggccggg
     1201 cactgccagc acttgcgtca tcaattgctc ggcagtgcgg tcgtcgctct gtaattgcgc
     1261 ggcgcgtgcg gcgtgccaga gaaacgacgc tttcgataac ggcgttgcgg cagttatcgc
     1321 gccacgcaga tacagttgct gcgcctgcgc gaactgttcg ttgccgaagt acgagcggcc
     1381 gatgtcgaag gtgagatcgt cgcgcttctg cggcatcgcc ttcagcgctg cggacaacag
     1441 cgccaccgca cggtcgaagt gccggtggtt ctgcatcgac tgtcccatca gatcgagctg
     1501 cgggccgttc atccgcgcga tcaattccgg acgatcaagc gcgcggctgg cgcgatcgga
     1561 tgcgtcatcg agcgttccgc cttgcagcaa ggccaggccg cgagcgagcg cgccgtttgc
     1621 gtctccagcg cgaaagtcga gctcgacgat gcgcgcgctc aaatcgcggc ggcgtgatgc
     1681 gcttgcagtt ggggcaagac gcgcggcgaa atcgctgaga gcgcgcaggt tgccaacgcg
     1741 cgcgagatgt tcgatctcgt cgtcgatggc ctgatcgcga tacggcgacg ctgccgcatc
     1801 gaagatcagc gtttgccggt cgcggctggc tgcggcgtga tcgccgcggt catcatcgat
     1861 ctccgcctga tggtagagag ccagggcgcg ccatggattc ccagccgcga gaaacggcgc
     1921 cagcttcgcc gcagcatctg ctggctcgtt gttttcgatc agcgcccgcg cgtggagata
     1981 cgcgagcgac catcgtgcgt attcgttcgg atcactcttc tcgatgcgag caagcagcct
     2041 ggcgagctcg ctccatcgtc cgttcacttc cagatcgcga aactgggccg ttaaggcact
     2101 cgccgagcgc tgttccgatg atgttgacgc cagccgcggc tctggcgcag cgcgaaatac
     2161 acgatggcgc gccgcgctct gccgcatgac gatgattgca acggcggcaa gaacgacgac
     2221 gacggcaacc gcggcaagag gaatggttaa gcgtttcttc acgaatcgtc tcgggccagc
     2281 gttcccgaaa atacagcccc gggagcgcgc ctgagaatct cgtgtttagc aatcgcgtag
     2341 ccaaggatgg cgtcgacgag catgcgccgg ccgtcgcgcg agaagaggat cagaaccttc
     2401 tgcttgatct cgcggatctc gacgacgtct tcccaccgca tccagcgtcc gcgtcttcca
     2461 aagtgtttca acacccagtt ctcgtacatc aggccgtcgt cgctgagata gatgtgatcg
     2521 ccgtactgca gcaccgccgc ggccagcgtc ccgacgaaga gcagaatcaa tccggccatc
     2581 ggaagcgcct cgatctcttt ttcgcccttc gatagatagt cgaacaccag cgccgccgac
     2641 gcgaacgatg ccgcgcagga gatgagccgc gcaaccggcg ttcgatcgaa cttgaggatc
     2701 cgggacatgg ggtggaaagt atatttgcgg tcttcgggtg tggggagtag
//
LOCUS       DATLVW010000344         3870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_424849, whole genome shotgun sequence.
ACCESSION   DATLVW010000344 DATLVW010000000
VERSION     DATLVW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424849"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VNN25_10460"
     CDS             <1..1052
                     /locus_tag="VNN25_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91991.1"
                     /translation="QAAVDTKTQPAVLDVPPLDLSVPESPAFTVLGVTPQDVVRPSTL
                     QEFGATVLNATDDKGNLQSGIALETNPYPLFKGAQLSLKDYQDPRNWHLRLLARLRTS
                     VATTKASGSDTAERAAVGVVIVPWDTGDPRLDPVLTKCINDALSFGDAQPVPIGEPGK
                     TTVTVLPAPTLKVIAACRSDFQERVADYWATSSWMIGAAPTWISDTGAAKKLSADGGG
                     IWTSLAIGINKDPQGHSHSQMILHARYRSNEQVKGSDSKYFGQDSQVLGARYRFGVPQ
                     FAGSIEASYVAQKPDGKPKDTFIRLIFGAERKVGESLWIQLALGREFDRTKGANGFVA
                     RSGFQYKFAQKSKYE"
     gene            complement(1077..3119)
                     /locus_tag="VNN25_10465"
     CDS             complement(1077..3119)
                     /locus_tag="VNN25_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXH91992.1"
                     /translation="MENTPIQIGWKRGDPSLDMLSREWLVTNALGGYASGTIGGANTR
                     RFHGFLIAALPAPAGRTMMFNHLEEILEGPDDLCWKLSGDEYGGREAALPEEGALEEF
                     AIENGKPVWRFNKGGIRIEKRVLMPHLQNTSYLVYRLIEGPPGLRLRLRPSLHFRPHE
                     GLLTAQLSDKWNVAINEKGCEIELTDGGGYPPLRLLLFGTKAYLQNDVRTFEHVLYRI
                     EKSRGYEHEGPLWSPGVIHADLELGVDCGIVASTDDWKVLKALPYAEALAAENERVAR
                     LLTISGHAADHFASTLVLAADQFIINPATRVADEARVRASGDEPRTVIAGYHWFTDWG
                     RDTMISLEGLTLTTGRNREAGNILRTFAAYIRDGLIPNMFPEGAHAGLYHTADATLWF
                     FHAVWRYAAATNDRETVRMLIPKLLDIVEHHLRGTLFNIHVDPADGLLAQGEEGYQLT
                     WMDAKVDGWVVTPRRGKAVEINALWYNALRILEALLRQENDDANAKIIDEHANRARVS
                     FNARFWYEEGQYLYDVVDAEGGGNDASLRPNQVFAISLDYPVLDESRWRRVVDVVKDE
                     LLTPVGLRSLSRGNPDYKKTYDGDLRARDAAYHQGTVWGWLIGPFIDAWLRVHPRDNA
                     GALAFLDGFRTELGDACIGTISEVFDAEAPFSPRGCVAQAWSVAEVLRTLRRTQSL"
     gene            3397..>3870
                     /locus_tag="VNN25_10470"
     CDS             3397..>3870
                     /locus_tag="VNN25_10470"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HXH91993.1"
                     /translation="MRRLLAAVIFLAALPLAAARLPKSVIPDHYAISITPDLAAAKFS
                     GQETIDVDIKEPVDTITLSSVGLELHDVVVASGSKLLNPTEITFDVPNEMASLKFGQT
                     LPPGKASMRISFNGALSEQLRGLYLSKTPRRNYAVTQFESTDARRAFPSFDEPAMK"
BASE COUNT          764 a         1176 c         1186 g          744 t
ORIGIN      
        1 ctcaggcggc tgttgacacg aagacgcagc cagctgttct agacgtgcca ccgctggatt
       61 tgtccgttcc agagtcgccg gcgtttacgg ttcttggagt gactccgcag gacgtcgttc
      121 gaccgtcgac ccttcaggaa ttcggtgcga ccgttctcaa cgccacagat gacaagggta
      181 atctacagtc cggcatcgca cttgagacca atccgtaccc tttgttcaaa ggggcgcaac
      241 ttagtctcaa ggattatcag gacccaagga attggcatct tcgtctactt gctcgtctgc
      301 gaacctcggt ggccacgacg aaggccagcg gaagtgatac tgcggagcgg gctgcggttg
      361 gagtggtaat tgtcccttgg gacaccggtg atccacggtt ggaccctgtg cttaccaaat
      421 gcatcaacga cgcactcagt ttcggtgacg cgcagcctgt gccaatcggc gaacctggga
      481 aaacgacggt cacagtcctt ccggctccaa ccttgaaggt gatcgcagcg tgccgatcag
      541 actttcaaga gcgtgtggcc gactattggg caacatcgag ctggatgatc ggtgcggcgc
      601 caacctggat tagtgatacg ggggccgcga aaaaacttag tgcggacggt ggtggaatct
      661 ggacctcact cgccatcggg atcaacaaag accctcaggg acattcgcac tctcagatga
      721 tccttcatgc gcggtatcgc tcgaacgagc aagtcaaggg cagcgattca aagtacttcg
      781 gacaggactc gcaggttctc ggcgctcgat atcgatttgg cgtcccgcag ttcgccggtt
      841 cgatcgaagc gtcatatgtt gcccagaagc cggacggaaa gccgaaagac accttcatca
      901 gactcatctt tggagcggag aggaaggtgg gcgagtcgtt gtggattcag ctcgccctcg
      961 gacgcgaatt cgaccgaaca aaaggtgcga acgggtttgt tgcgcgtagc gggtttcaat
     1021 acaagttcgc tcaaaagtcg aagtacgagt gacagcgcag tcatcgattt gaggatttag
     1081 agactctgag ttctccgcag agtcctcagc acttccgcca cactccacgc ttgcgccacg
     1141 catccgcgcg gactgaacgg cgcctcggca tcgaagactt cgctgattgt gccgatgcag
     1201 gcgtcgccca gctcggtgcg gaaaccgtcg agaaaggcca gcgcgccggc attatcccga
     1261 ggatgcactc ttagccacgc gtcgatgaac ggcccgatca gccagcccca gacggtcccc
     1321 tggtgatacg cggcgtcgcg ggcgcgaaga tcgccgtcgt aggttttctt gtaatccgga
     1381 ttccctcggg acaatgaacg cagaccgacc ggcgtgagca actcgtcctt caccacatcg
     1441 acgacccggc gccagcggct ttcatcgaga acgggatagt caagcgagat cgcgaagact
     1501 tgattggggc gaagcgaagc atcgttgccg ccgccctcgg catccacgac atcgtaaaga
     1561 tactgacctt cttcgtacca gaagcgggcg ttgaaagaga cgcgggcgcg gttggcgtgt
     1621 tcgtcgatga tcttggcgtt ggcgtcatcg ttttcctgcc gcagcagcgc ctcgagaatg
     1681 cgcagcgcgt tgtaccagag cgcgttgatc tccaccgcct tgccgcggcg cggtgtgacg
     1741 acccagccgt cgaccttcgc gtccatccag gtgagttgat atccctcctc gccctgagca
     1801 agcaagccat cggcgggatc gacatggatg ttgaaaagcg tgccgcgcag atggtgctcg
     1861 acgatgtcga ggagcttggg gatgagcatg cgcaccgtct cgcggtcgtt cgttgcggcg
     1921 gcgtagcgcc agacagcgtg gaagaaccag agcgtagcgt cggcggtgtg atacagcccg
     1981 gcatgggctc cctcggggaa catattcggg atcaggccgt cgcggatgta cgccgcgaat
     2041 gtgcgcagga tgttgccggc ctcgcggttg cgtcctgtcg tcaacgtcag cccttccaga
     2101 ctgatcatcg tgtcgcggcc ccagtctgtg aaccagtggt agccagcgat cacagtgcgg
     2161 ggctcatcac ccgaggcgcg gacgcgagcc tcgtcggcaa cgcgggtggc gggattgatg
     2221 atgaattgat ccgcggccag aacgagcgtc gaagcgaaat gatccgcggc gtggccggag
     2281 atcgtgagca gccgcgctac gcgctcgttt tccgccgcca gcgcctcggc gtacggcagc
     2341 gccttcaaca ccttccaatc gtccgtcgac gcgacgatgc cgcaatcgac gccgagctcg
     2401 aggtcggcgt gaatcacgcc cgggctccac agcggccctt cgtgctcgta gccgcggctc
     2461 ttctcgatcc gatagagcac atgctcgaac gtccgcacat cgttttgtag atatgccttc
     2521 gtgccgaaga gcaggaggcg aagcggcgga tagccaccgc catccgtcag ctcgatctcg
     2581 catcccttct cattgatcgc gacattccac ttatcggaca actgcgcggt cagcaaacct
     2641 tcgtgcggac gaaaatgaag cgatggacgc aatcggagcc gtaacccggg cggtccttcg
     2701 atcagccgat agacgaggta ggacgtgttc tgcaggtgag gcatcagcac gcgtttctcg
     2761 atgcggatgc cgcctttgtt gaaacgccag accggcttgc cgttctcgat cgcgaactct
     2821 tcgagcgccc cctcctcggg aagcgcggcc tcgcgcccgc catactcatc gccgctgagc
     2881 ttccagcaga gatcgtccgg accttcgagg atctcctcga gatgattgaa catcatcgtc
     2941 cgcccggccg gagcggggag agcggcgatg agaaacccgt gaaagcgccg cgtgttcgcg
     3001 cccccgatcg tgccggaggc gtatccgccg agggcgttgg taacgagcca ctcgcgcgaa
     3061 agcatgtcga gggaggggtc tccgcgtttc cagccaatct ggatcggtgt gttttccatc
     3121 aggagcgagg tctgccgaac ttatgcccgt ttggagtgca ggcgtctcgc ctgcggccgg
     3181 cgggcgtctc gcccgtcggt aagagcgcga agaagtcagt gagagaactt agcaagcagc
     3241 ccgagaaaac cccctgccgg tgaatcttcc gccatacgcc cgcacgatct ccgcctcgag
     3301 ccgagtttgc tcaatcgcgc acgatacaca cgccgaggcg aggacgcctc gacgccgcag
     3361 gcgagacgcc tgcactcctt gcgctatcct tcccgtatgc gccgccttct cgctgctgtc
     3421 atctttctcg ccgctttgcc gcttgctgcg gcccgtctgc cgaagagtgt gattcctgat
     3481 cattacgcga tcagcatcac gcctgatctt gctgccgcga agttcagcgg gcaggagacg
     3541 atcgacgtcg atatcaaaga gccggtcgat acgatcacgc tcagcagcgt cgggctcgaa
     3601 ttgcacgacg tcgtggtcgc cagcgggagc aagctgctga atccgacgga gatcaccttc
     3661 gatgtgccga atgagatggc ctcgctcaag ttcgggcaga cgctgccgcc gggcaaggcg
     3721 tcgatgcgca tcagcttcaa cggcgcgctg agcgagcaac tgcgcgggct ctatttgagc
     3781 aagacgccgc ggcgcaacta cgcggtcacg caattcgaat cgaccgacgc acgccgcgcg
     3841 tttccgtcgt tcgacgagcc ggccatgaag
//
LOCUS       DATLVW010000345         6777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_58480, whole genome shotgun sequence.
ACCESSION   DATLVW010000345 DATLVW010000000
VERSION     DATLVW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58480"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VNN25_10475"
     CDS             <1..975
                     /locus_tag="VNN25_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXH91994.1"
                     /translation="AKGLSNADSALFPNAISADLAKIPFIAAFDRARGRGVLYNDDRV
                     LLASGDFAVAARYGDLPMIAPGKSSEALGNAIAKNGVVVSESFSLKYNKGVGDNITLT
                     TIHGRRSFPITGVYRDYSNDRGVVAMDRGLFIRSFDDDAISTVVIYLKPGVDRDTARR
                     NLETTLGPKYHAFAITNGEIRGEVMKIFDQTFLITYALLAVAIVVAVLGVINTLAALI
                     LERKRELALLRVLGMTVAQVRRMLVLESSVLGLTSTIAGLAMGYVLSWILIYVINKQS
                     FGWTIAFHTPVKLIATSLAVTFGASLVAGLVPSRLARRIDLANAMKAE"
     gene            1178..1390
                     /locus_tag="VNN25_10480"
     CDS             1178..1390
                     /locus_tag="VNN25_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXH91995.1"
                     /translation="MRNTGKVKWFNDSKGFGFITPDDGGKDCFVHHSAIQGSGFKSLA
                     EGDHVEYDVEQGQKGPAAANVTKIPG"
     gene            complement(1398..2051)
                     /locus_tag="VNN25_10485"
     CDS             complement(1398..2051)
                     /locus_tag="VNN25_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91996.1"
                     /translation="MRRGLRLFLPLTIVAATLVTAMVAAPPRFGAREPLTIEGAAALM
                     QNTAVIASLLAIWMLAAVALTLLSGREIRYSSIEMRASALHCFALGLVAVTSFVLTAI
                     VFSYLIPYHVGVPLLFALAVIAILTKIYGTIAVFHAVGTLVAGSRTREQLAGRRWLRG
                     DLAMVVVGVIVLGALRLIPIAGPIIWAFASVFGIGTALATKFGRREPWFLAWRAVES"
     gene            complement(2044..2487)
                     /locus_tag="VNN25_10490"
     CDS             complement(2044..2487)
                     /locus_tag="VNN25_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH91997.1"
                     /translation="MTDERCQRYLEDPEANAGHLETCAECRAENAALAAKSDVDVAPI
                     SLDALPLAPWEGSSHRAWPLVLGAAAAVILIAFVLCDLAGISPLHVAESSLSSIEARR
                     GLLRNATEWLRAAPAAMQVAFGVAFIVVNALLVVLLRRAPRGIDA"
     gene            complement(2484..3068)
                     /locus_tag="VNN25_10495"
     CDS             complement(2484..3068)
                     /locus_tag="VNN25_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH91998.1"
                     /translation="MAEREQLDDRTLVARVVEGDRDRFTELVKRYEKRVINYVYRITH
                     RYEDAHDLTQEIFVKVYLALDRFDPKYQFSTWLFRIAQNSAIDALRKKSVSEVSIAAK
                     PGDEDSKDREFADTGISPYRALNNKQLADAIDTAVTNLPADYRELIQLRHFAELSYEE
                     IASMKKLPLGTVKNKLFRARNLLKVELEGFVEPL"
     gene            3150..3872
                     /locus_tag="VNN25_10500"
     CDS             3150..3872
                     /locus_tag="VNN25_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH91999.1"
                     /translation="MIEIRNLTKRYGDFTAVDDISLDVARGEIFGFLGPNGAGKTTTI
                     RILAGLSLPSSGSVRVNGLDVTKDGVRVKATTGYIPDRPFLYEKLTGRELLQFVSNLY
                     GREWRDCETRAMELLAYFGIANWADARIENLSHGMKQKLVIVSALVHDPELLIIDEPM
                     VGLDALAQKQVKLLFRRLAAEKKTVFLTTHTLSVAEAVCDRIAILNRGRIVASGTTNE
                     LKSDRALEDVFLELTYSDESSI"
     gene            4245..5048
                     /gene="prmC"
                     /locus_tag="VNN25_10505"
     CDS             4245..5048
                     /gene="prmC"
                     /locus_tag="VNN25_10505"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXH92000.1"
                     /translation="MTETIASLRARYRELATAKHVSPRDVDVLLADVTGHTAPWVFAH
                     GEEEIDPGPFIALIERRLRGEPLQYIRGKSEFYGRDLRVDERVLIPRPDTEILVEAAI
                     ARAPGGGRVLDVGTGSGCVAISLERERPDLEVVAIDISFAALVVASSNARTYRSRVRF
                     ATSDALDSIRGEFDLVVSNPPYIPAAEVEQLDVEVRDHEPRVALTAGPLGTEVIKRVL
                     EARRAPLVMFEIGFGQLGDLRHVARGYDIIDVTNDLAGIERVVTLRRRA"
     gene            complement(5077..5559)
                     /locus_tag="VNN25_10510"
     CDS             complement(5077..5559)
                     /locus_tag="VNN25_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92001.1"
                     /translation="MNHRSSVPFLIGVVTILMVLPLHAATTGKVSGAFVLAGSDAQLK
                     YVRATRVKLDEKKSGYAVVISAEPPTGEIMDWKTAEPSKKGNFIHIMFESNGDVWVTE
                     LGHVKSAAGRFGVVTEVHKVTFDVRDGRLAAHVKTDGDQKFDDPYNVDLTFEAPLEEK
                     "
     gene            5640..5993
                     /locus_tag="VNN25_10515"
     CDS             5640..5993
                     /locus_tag="VNN25_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXH92002.1"
                     /translation="MPANDRCLFCRIASGEIATKKICEDDQVVAFNDINPQAPTHVLV
                     IPRKHIASLDDLNEEDAAIIGTTLVRVSEIARMLKLNGEGYRVVANHGEDAGQTVFHI
                     HFHLLGGRNFSWPPG"
     gene            complement(6147..>6777)
                     /locus_tag="VNN25_10520"
     CDS             complement(6147..>6777)
                     /locus_tag="VNN25_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXH92003.1"
                     /translation="IADRHSETDTMEEMFSDLFIPTLTFAQEELDRKRITSANDHFIK
                     DVIRELIIRFGDRNAGVPDPERHIVAVSVAGERLSLGTLMLTQLLREEGFSIDYFTDL
                     PDAELISFVNEVKPEAVFVSCTNPDHLEAGYGLLQLIAASFVDLMIGAGGSAFARDRA
                     KTLSAGATYVPTTLVEAKEDFLTRRKAARRKISRSITFSGTRFRVPPSA"
BASE COUNT         1303 a         2095 c         2132 g         1247 t
ORIGIN      
        1 gcgaaagggc tttcgaacgc cgactccgcg ctctttccga atgccatctc tgccgatctc
       61 gcgaagatcc cgttcatcgc cgcgttcgac cgcgcgcggg gccgcggcgt gctgtacaac
      121 gacgatcgcg tcctgctcgc cagcggcgac ttcgctgtcg ccgcgcgcta cggcgatctg
      181 ccgatgatcg cgccgggtaa atcgtccgag gcgctgggca acgcgatcgc gaagaacggc
      241 gtggtcgtgt cggagagctt ctcgctgaag tacaacaaag gcgtcggtga caacatcacg
      301 ctaacgacga tccacggccg gcgctctttc cccatcaccg gcgtctaccg cgactattcg
      361 aacgaccgcg gcgtcgtggc gatggaccgc ggcctcttca tccgctcctt cgatgacgat
      421 gccatcagca cggtcgtcat ctacctgaaa ccaggcgtcg accgcgacac tgcgcggcga
      481 aacctcgaaa caaccctcgg accgaaatac cacgccttcg ctatcaccaa cggcgagatc
      541 cgcggcgaag tgatgaaaat cttcgaccag acgttcctga tcacctacgc cctcctcgcc
      601 gtcgccatcg tcgtcgccgt cctcggcgtg atcaacaccc tggccgccct gatcctggag
      661 cgcaaacgcg agctcgccct cctccgcgtc ctcggcatga ccgtcgccca ggtccgccgc
      721 atgctcgtcc tcgaatcctc agtcctcggc ctgacctcaa ccatcgccgg cctggccatg
      781 ggctacgtcc tctcctggat actgatctac gtcatcaaca aacagtcctt cggctggacg
      841 atcgcgttcc acacaccggt gaaactgatc gcgacctcgc tggcggtgac gtttggggcg
      901 tcgctcgtcg cgggtctcgt cccttcgcgt ttggcaaggc gaatcgattt ggcgaacgcg
      961 atgaaggcgg agtagagcgg cgcaaggact ctctcacgcg aagtcgcgaa gacgcgaaag
     1021 gtaatgaggg ttgggatgct gtccttcgcg acttcgcgac ttcgcgtgag tggatcgcgg
     1081 ctcgcgaaga ggtgagattc aacccgcctc gctacgtcaa atcccgccga gtagtgtact
     1141 ttgtcgccct acgtttcatg ttcgaaagag gaaaagcatg cgcaacacgg gaaaagtgaa
     1201 gtggttcaac gacagcaagg gtttcggatt catcactcct gacgacggcg gcaaagactg
     1261 cttcgttcat cactcggcga tccagggctc ggggttcaag agcctggccg agggcgacca
     1321 tgtggagtac gacgtcgagc agggccagaa gggtccggcc gctgccaacg tgacgaagat
     1381 cccgggatag acgtaactta cgattcgacg gcgcgccaag ccaggaacca tggctcgcgc
     1441 cggccgaatt ttgtcgccag tgcagtgccg atgccgaaga ccgaggcgaa ggcccagatg
     1501 atggggccgg cgatcgggat cagacgcaag gcgccgagca cgatgacccc gacgacgacc
     1561 atggccagat cgccgcgcag ccagcggcgg cctgccagtt gttcgcgcgt tcggctgccc
     1621 gcgacgagtg tgcccacggc gtggaacacg gcgattgtgc cgtagatctt ggtcagaatg
     1681 gcgatcacgg cgagggcgaa gagcagcggc acgccgacgt ggtacgggat caggtagctg
     1741 aagacgatcg ccgtcagcac gaagctcgtc acggccacga gtccgagcgc gaagcaatga
     1801 agcgcgctgg cccgcatctc gatggaggag taacggatct cacggccgct cagcagcgtc
     1861 agcgcgaccg cggcaagcat ccagatggcg agaagcgacg cgatcacggc ggtgttctgc
     1921 atcagcgctg cggcgccctc gatcgtaagc ggctcgcgag cgccgaatct cggcggcgcg
     1981 gccaccatcg ccgtcaccag tgtggcggcg acgatcgtca acggcaggaa cagacgcaac
     2041 cctctacgca tcgatccccc tcggcgcacg ccgcagcagc acgacgagca gcgcgttcac
     2101 gacgatgaat gcgactccga aggcaacctg cattgccgcc ggagccgcgc gcagccactc
     2161 ggtggcgttg cgcagaaggc cgcgccgcgc ctcgatcgac gacagcgagc tctccgcgac
     2221 gtgcaacggc gagatcccgg caagatcgca gaggacgaaa gcgatgagga tgacggcggc
     2281 agcagcgccg agaaccagtg gccacgcgcg atgcgacgaa ccctcccacg gtgccagcgg
     2341 aagcgcatcg agggagatcg gcgcgacgtc tacgtccgac ttggccgcca gcgcagcgtt
     2401 ctcggccctg cattccgcac acgtctccag atgcccggca ttcgcctcgg gatcttccag
     2461 gtaccgctga cacctttcgt cggtcatagg ggttccacaa atccttcgag ctcgaccttc
     2521 agcaggttgc gcgcccgaaa cagtttgttc ttcacggtgc cgaggggaag cttcttcatc
     2581 gaagcaatct cctcatagga tagctccgcg aagtgccgaa gctgaatcag ctcgcgataa
     2641 tcggccggca gattcgtgac tgccgtgtcg atggcatcgg ccagttgctt attgttgagc
     2701 gcgcggtacg gtgagattcc ggtgtcggcg aactcccgat ccttcgagtc ttcgtcgcca
     2761 ggcttggctg cgatcgagac ctcgctgacc gacttcttgc gcagcgcgtc gatggcgctg
     2821 ttctgcgcga tccgaaagag ccacgtcgaa aactggtatt tcgggtcgaa acgatcgagt
     2881 gcgaggtaga ccttcacgaa gatttcctgg gtcagatcgt gtgcgtcttc atagcgatgc
     2941 gtgatccggt acacgtagtt gatgacgcgc ttttcatagc gcttcaccag ttcggtgaac
     3001 cggtctcggt cgccctcgac gacgcgcgcc acaagggtac gatcgtcgag ttgctcacgt
     3061 tcagccattc gtggttacgg ttcccgcggg tgaaagttct tcgagacgga tgataccctg
     3121 ccgcccccga accgtctgat gatcgtccga tgatcgagat ccgcaatctg acgaagcgct
     3181 acggcgactt caccgcggtc gatgacattt cgctcgatgt cgcccgcggc gagatcttcg
     3241 gctttctcgg gccgaacggc gccggaaaga cgaccacgat ccgcattctt gccggccttt
     3301 cgcttccttc ctcgggctcg gttcgggtca acgggctgga cgtgacgaag gacggcgtgc
     3361 gcgtgaaagc tacgaccggc tatatccccg accggccgtt tctctacgag aagctgacag
     3421 ggcgcgagct cctgcagttc gtgtcgaatc tctacggccg cgagtggcgc gactgcgaga
     3481 cgcgggcgat ggagttgctg gcgtacttcg gaatcgccaa ctgggcggac gcgcgaatcg
     3541 agaatctctc gcacgggatg aagcagaaac tcgtcatcgt ctcggcactc gtccatgacc
     3601 cggaactgct gatcatcgac gagccgatgg tgggactcga tgcactggcg cagaagcagg
     3661 tgaagctgct gttccgccgg ctggccgcag agaagaagac ggtcttcctg acgacgcata
     3721 cgctatcggt tgccgaggcg gtgtgcgacc ggatcgcgat cctcaaccgc ggccggatcg
     3781 tcgccagcgg cacgacgaac gagttgaaga gcgatcgtgc gctggaggat gtttttctgg
     3841 agttgacgta tagcgatgag tcttcaatat gagtgcatat gccgcgtttt cagatagttc
     3901 ggacgaggcg cggtcgatcg ctctgcgcac ttacgccccc ctcatccggc cttcggccac
     3961 cttctccccc cgcaaaaagc gcggggggag aaggcgctcg attggagagc actccaagga
     4021 aaaaggaaga ggtgtggcaa gaggtgtgga aaggaggggc ggcaggcgtg tgttcgagcc
     4081 ctgcacttct cacacttgca gacgattgct ggcagtgtct ttcaatcgag cgccttctcc
     4141 cccacgcttc tcagtggggg agaaggtggc cgaaggccgg atgagggggc gctggtctct
     4201 cgcctcgaag gcgcaggacg gcgaaggatc tcagccacgt aacaatgact gaaacgatcg
     4261 catcgcttcg tgcgcgctac cgcgagctgg ccactgctaa gcacgtcagt ccgcgcgatg
     4321 tcgacgtcct gctcgcggac gttaccgggc acacggctcc ctgggtcttc gcgcatggcg
     4381 aggaggagat cgatccgggg cccttcatcg cactcatcga gcgccgtctg cgcggcgagc
     4441 cgctccagta catccgaggc aaatcggagt tttacggacg cgatcttcgt gttgacgagc
     4501 gcgttctgat cccgcgtccg gacactgaga tcctcgtgga agcggcaatt gcacgcgcgc
     4561 ccggaggagg acgcgtgctc gatgtcggta ccggcagcgg atgcgtcgcc atctcgttgg
     4621 agcgcgagcg gccggacctg gaggtggtgg cgatcgacat ctcctttgcc gcgctcgtcg
     4681 tcgcttcgtc gaatgcacga acctaccgct cgcgcgtccg ctttgccacc tccgatgcgc
     4741 tcgacagcat ccgcggcgag ttcgatctcg tcgtctcgaa cccgccgtac attccggcag
     4801 ccgaggttga gcagctcgac gttgaggtgc gcgatcacga gccgcgcgtg gcgctgactg
     4861 ccgggccgct cggaaccgaa gtgatcaagc gcgttctcga ggcgcgccgc gcgccgctgg
     4921 tgatgtttga gatcggattc ggacagctcg gcgatctgcg gcacgtcgcg cgcggctacg
     4981 acatcatcga tgtcacgaac gatctcgccg gaatcgagcg ggtggtcact cttcgacggc
     5041 gcgcctgacc tggcgaccca gtaccagcca gccctgctac ttctcctcga gcggagcttc
     5101 gaacgtcagg tcgacgttgt aggggtcgtc aaacttctga tccccatcgg tcttgacgtg
     5161 tgccgcgagg cggccatcgc gcacatcgaa ggtcaccttg tgtacctcgg taaccacgcc
     5221 gaaacgtcca gccgctgact tgacgtgacc gagctcggtg acccacacat ctccattcga
     5281 ttcgaacatg atgtgaatga aattaccctt ctttgatggc tccgcggttt tccaatccat
     5341 gatttcgccg gtgggcggtt cggcagagat caccacagcg tagccagact tcttttcgtc
     5401 gagcttgact cgcgttgcgc gcacgtattt gagttgcgca tcggacccgg cgagcacgaa
     5461 tgctcccgaa actttgcctg tcgtggcggc atggagcgga agaaccatga ggattgtcac
     5521 aacaccgatc agaaacggca ccgatgagcg gtgattcata ggctgtccct caggggtcgg
     5581 attatatgcc gcgcgctgac gtcaattgat cgtcagatcg aacgtcgtat catttctaca
     5641 tgcctgcaaa tgaccgctgc ctcttctgcc gcatcgccag tggcgagatc gccacgaaaa
     5701 agatctgcga ggacgatcag gtagtcgcgt tcaacgacat caacccgcaa gcgccgacac
     5761 acgtgctggt cattccgcgc aagcacatcg catcactcga cgatctgaac gaggaggacg
     5821 ccgccatcat cggcacgacc ctcgtgcggg tgtcggagat cgcccgcatg ctcaagctga
     5881 acggcgaggg gtatcgcgta gttgccaatc acggcgaaga cgcaggccaa accgtgttcc
     5941 acatccactt ccatctcctc ggcggacgca atttcagttg gccccctgga taattgcgcg
     6001 gcccgggggg ccgcgcgttg tcggttgtcg gtcagttgcg tcgctggcgc gacgcgttgt
     6061 cagagacgga ccaaagtggg cgacaacgcg cgaagcgcaa ccaaccgaca accgacaacg
     6121 cgcgcagcgc aatcagccag cgcaattcag gccgatggtg gtacgcggaa acgcgtcccg
     6181 ctgaacgtga tcgagcgcga gatcttgcgg cgagcggctt tgcggcgcgt caggaagtct
     6241 tcctttgctt ccacgagcgt cgtcggaacg taggtcgcgc cggcggagag ggtcttggcg
     6301 cggtcgcgtg cgaaggcgga gccgcctgcg ccgatcatca gatcgacgaa cgaagcggcg
     6361 atcagttgca gcaggccgta gccggcttcg aggtggtcgg gattggtaca ggagacgaac
     6421 accgcctcgg gcttcacttc gttgacgaac gaaatcaact ccgcgtccgg caggtcggtg
     6481 aagtagtcga tcgagaagcc ttcctcgcgg agcaactggg tcagcatgag cgtgccgagg
     6541 gagagacgct cgccggcaac ggagacggcg acgatgtggc gctccggatc gggaacgcct
     6601 gcgttgcggt cgccgaagcg gatgatcagc tcgcggatca catccttgat gaagtgatcg
     6661 ttggccgagg tgatgcgttt gcggtcgagc tcttcctgcg cgaaggtcag cgtcgggatg
     6721 aagaggtcgg agaacatctc ctccatcgtg tctgtctcgg aatgacggtc ggcgatg
//
LOCUS       DATLVW010000346         6427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_141511, whole genome shotgun sequence.
ACCESSION   DATLVW010000346 DATLVW010000000
VERSION     DATLVW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141511"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VNN25_10525"
     CDS             complement(<1..908)
                     /locus_tag="VNN25_10525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH92004.1"
                     /translation="MPNYDLIIIGGGIIGGSTLYHLAQLGYGGRVLVLEREDALSKGS
                     TALSAGGFRNIWTTPINMRLTSYSIAKLKSFQDELQFPIGFDQRGYLFCQYEENFESA
                     RAYGSEWAKNGVTIEFLDATEVEAKVPGLRAGIGHIDPEVNEILQMKPIAGGVFGPDC
                     GSFDPSAAAQGYFERGLEGFAGRAELRLNATVKKLRIIDGKYRGVEMVDGSFIESEKI
                     LVAAGAWTNALMEASGLAGDDLPPVIPLNRMLFITNMPPIEGFERIPMTIIDNGIYFR
                     PEGPNLMIGRADPDTPASFDTTADAK"
     gene            complement(1622..2347)
                     /locus_tag="VNN25_10530"
     CDS             complement(1622..2347)
                     /locus_tag="VNN25_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXH92005.1"
                     /translation="MLQTLSLIGRETARIVLHASCVVCDGDLPWRSRKASCCAACWAS
                     LPQIPATRCRYCAAPWSGNDPGYVCGSCLATPPPVEWTEAWGCYRGSLEKVLHAFKFK
                     RHDFFAAPLAELVESVVRERGDLDFDAIVPVPMHRAKLRRRGYNQAELLARALSRRLG
                     IPCETALLTKTRDSGAQSRLARNERAANVRDAFRASPRAASRRILLVDDVITTGETIR
                     ACARALTDAGAKRIAAAVVAKTE"
     gene            2488..3039
                     /locus_tag="VNN25_10535"
     CDS             2488..3039
                     /locus_tag="VNN25_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92006.1"
                     /translation="MKKTLILLGFLLVTAFPVLAQNHELTILAGGAKALKTFDGGKSD
                     FQHGFEEISYGIEMEQNTILKINVARMDARTVFVKKDADGHPTADLVDPKGQVEYADA
                     TIEYRFAEPFGYTGLFAGTGIYRQSSGSRQDTDYGFVGGVNGIFPISRSVGITAEAAY
                     HWVHFYSPKPRFGTVGVGLRFAF"
     gene            3099..3560
                     /locus_tag="VNN25_10540"
     CDS             3099..3560
                     /locus_tag="VNN25_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH92007.1"
                     /translation="MAKDSNQKSGSRSARDVMTANPSTVSESDSIRDVARIMASEDTG
                     VVPVVDGKKVIGIVTDRDIVVRLVAEGKDTGDAKVKSYMSRAVRSVRDDTPVSEVLSV
                     MSNAQIRRVPVVNASDELVGIVSMRDVSTETNQNANVGKAVEQISQGPANN"
     gene            3621..4841
                     /locus_tag="VNN25_10545"
     CDS             3621..4841
                     /locus_tag="VNN25_10545"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXH92008.1"
                     /translation="MPKRVALALFLVAVTPLYAARPVVKPVPKVAPAPVGITVDTHAD
                     TPTEFLDHPFDLGALNTRGHFDYPRMKAGGLDAEFFAAYVPAKYANKGAAAFCLKIME
                     TIHEMVDAYPTWVRFATSTAGIKAAVADNRRAILIGIEGGHAIEDSLDILRAFYRFGA
                     RYMTLTHTNTNNWADSSGDEAIHNGLTPFGKEVVLEMNRLGMLVDISHVSDKTFYDVI
                     EVSKAPVIASHSSSRALAKAPRNMTDEMLVALARNGGVAMVNFYPVFLSDEVAKASKE
                     REERLKPEIAALKAKDPSEGAVYEEGLRKLMAANPLPKVSWTVIVDHIDHMVKVAGID
                     HVGIGSDFDGIPEVPEGMEDISKLPAIRTELKRRGYADADVRKIMGENFMRVFGEVEK
                     IAKELQAKEPAAAK"
     gene            complement(4983..6305)
                     /locus_tag="VNN25_10550"
     CDS             complement(4983..6305)
                     /locus_tag="VNN25_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92009.1"
                     /translation="MNEHPDRTTLAKYLEGDLVGDRLAITTHLADCADCRNELDALAA
                     TFRLHDNVLDFIESRKSDRERPLEQFDILATRQRMGAEAARAEAFFGELMRLPIEEWL
                     SAFAENPHQRSEAMVLRLLTEVEIEVKRRPQHALLLINVAETVTGSLDIPAIHVCAGD
                     CWKQRANALRHLGRYSESLEAADLAAAFYRLLPLGDFDIGQALYTRAGTLFKMTLYGE
                     ALSVLESARELLREFGDTMPLAKTLMLDAAIRFEQGDVVRAEQTWLEVVPMLKRFDDR
                     MELARVRANLAECSLRRGRPAKALAEAQAAATGFAELQMDAERIRSEWTVAAILRALG
                     EHDRALELLYAAAASFRALGMTSDAGFVNLDITEELLRRKEWSEAANIARELAGLFTA
                     AGVTLASVTALSYLRTAVEGERATPALVRYVRDYVAADDPARTFSAPS"
BASE COUNT         1211 a         2013 c         1981 g         1222 t
ORIGIN      
        1 tacttcgcgt cggcggtggt atcgaaactg gccggcgtat cggggtccgc gcgtccgatc
       61 atcaagttcg gtccctcggg acgaaaatag atgccgttgt cgatgatcgt catcggaatc
      121 cgctcgaagc cttcgatcgg cggcatgttc gtgatgaaga gcatgcgatt gagcgggatg
      181 accggcggca ggtcgtctcc ggccaggccc gacgcttcca tgagcgcgtt cgtccacgcg
      241 cccgcggcga cgaggatctt ttcgctttcg atgaacgagc cgtccaccat ctccacgccg
      301 cgatacttgc cgtcgatgat gcgcagcttc ttaaccgtcg cattcagccg cagctccgcg
      361 cgtccggcga atccttccag cccgcgctcg aagtatccct gcgcggccgc cgagggatcg
      421 aagctgccgc agtcgggtcc gaacactccg ccggcgatcg gtttcatctg caggatctcg
      481 ttcacctcgg gatcgatgtg tccgatcccg gcgcgcagcc ccggcacctt cgcttcgact
      541 tccgtcgcgt cgagaaactc gatcgtcacg ccgttcttcg cccactcgct gccgtaggct
      601 ctggcgctct cgaaattctc ttcgtactgg cagaagaggt agccgcgctg atcgaatccg
      661 atcggaaact gaagctcatc ttgaaacgac ttcagcttcg cgatcgaata gctcgtgagt
      721 cgcatgttga tcggcgtcgt ccagatgttg cggaagcctc cggcggagag tgcggtcgaa
      781 cctttcgaca acgcatcttc gcgctcgagc acgagcacgc gtccgccgta gccgagttgc
      841 gccaggtggt agagcgtgct cccgccgatg atcccgccgc cgatgatgat caggtcgtaa
      901 ttgggcattg attctccggg aaaagcagag acaagggact agagatcaga gatcaggggg
      961 agtcgtcaag ttttctgatc tgtgtgaagc aagagacaag agattagaga tcagagacga
     1021 ctggaacctt cgcgcccctg atctctagtc tctggtctcc tgacttctgc attctcattc
     1081 tgtcttcgct actcgtgaaa cagagacaag ggatcagaga tcagagatga ggtgcagcca
     1141 tctttaactg atctcttgtc tctgatcaca agaggctaga gatcagcagg taggcgccag
     1201 atccttatct catgtcgctg atctcatgtc tctgatctcc tcctgattct ttcgccgcac
     1261 tcctgaatcg agcgccttct cccctcgggc ttctgcgagg ggagaaggtg ccgaaggcgg
     1321 atgaggggtg tcgcgcaatc gaacggcatg gggttctaac ctccgtgagt gctccatgtc
     1381 tgcaacgttt tcgcgagagt cgacgccccc tcatccggct tcgccacctt ctcccccaca
     1441 aaagcgtggg ggagaaggcg ctcgattgga agggtgctgc tgacaacctc tcgccgcact
     1501 cgattgagac agtggcgctg acaaacctca tgagctatgt agcctgatct cttgtctccg
     1561 gttgtctctg atctcttgtc tccgcttctc tgatctctag tctctgcttt tctgatctct
     1621 gttactccgt cttcgcaacc accgccgctg caatgcgttt cgcgccagcg tcggtgaggg
     1681 cgcgtgcgca ggcgcggatc gtctcaccgg tcgtgatcac atcatcgact aggaggatgc
     1741 gccgcgatgc tgcgcgcggc gaggctcgaa aggcgtcgcg cacgttcgct gcacgttcgt
     1801 tgcgggccag cctcgattgg gccccgctgt cgcgcgtctt cgtcaggagc gccgtctcgc
     1861 aggggatgcc gaggcggcgg gaaagggcgc gggcgaggag ctcggcctgg ttgtagccgc
     1921 gcctgcgcag tttcgcgcga tgcatcggaa cgggaacgat ggcgtcgaaa tcgagatcgc
     1981 cgcgttcccg gacgacgctc tcgaccaatt cggccagcgg cgccgcgaag aaatcgtggc
     2041 gcttgaactt gaacgcgtgc agaacctttt cgagcgagcc ccggtagcag ccccacgcct
     2101 cggtccactc gaccggcggc ggtgtggcga ggcacgatcc gcagacgtaa ccgggatcgt
     2161 tcccgctcca tggcgccgcg cagtaacggc agcgcgtcgc cggaatctgc ggcagcgacg
     2221 cccagcacgc ggcgcagcag gaagccttcc gcgacctcca cggcaggtcg ccgtcgcaca
     2281 ccacgcacga ggcgtgaaga actattctcg ccgtctcccg tccaattaag gagagtgttt
     2341 gcaacatgag tggattgtat cgggtgtggg gggtggggag tggggtgtgg gagttaatcg
     2401 tcgaagttgt tttcaaatac acctcttcga ctcccacacc ccacccccca ctccccaccc
     2461 ccctaacttc gatatagtga ggcctgcatg aaaaagactc tgatactcct cggctttctc
     2521 ctcgttaccg cgttccccgt tctcgcccag aaccatgagc tcacgatcct cgcaggagga
     2581 gcgaaggctc tgaagacgtt cgatggcggc aagtccgact tccagcacgg gttcgaagag
     2641 atctcgtatg gaatcgagat ggagcagaac accatcttga agatcaacgt tgcgcggatg
     2701 gacgccagga ccgtcttcgt aaagaaagat gccgatggtc atccgaccgc cgacctcgtc
     2761 gatccgaagg gacaggtcga gtacgccgac gccacgatcg aataccggtt cgctgagccg
     2821 ttcgggtaca ccggcctctt cgcgggaact ggcatttacc gccagagcag cggcagccgg
     2881 caggacaccg actacggttt cgtcggcggc gtgaatggga tcttccccat ttcgcgcagc
     2941 gtcggcatca ccgcggaggc ggcgtatcac tgggtccact tctacagccc gaagccgcgt
     3001 ttcggaactg tcggcgtcgg tcttcgcttc gcgttctgat atttgattcg gggcacgcgc
     3061 cttgctcctt cgcctggtga aacaggggag gatcatctat ggccaaagac tcgaatcaga
     3121 aatcaggctc gcgcagtgcg cgcgacgtca tgacagcgaa cccaagcacc gtgtccgaat
     3181 cggactcgat ccgcgatgtt gcgcggatca tggcgagtga ggataccggc gtcgtgcccg
     3241 tcgtcgacgg caagaaggtc atcgggatcg tgaccgaccg cgacatcgtg gtccggctcg
     3301 tcgccgaagg gaaagacacc ggcgacgcga aagtgaagtc gtacatgtca cgggccgtgc
     3361 ggtccgtccg ggacgatacg cctgtgtccg aggtgctcag cgtgatgagc aacgcgcaga
     3421 tcaggcgcgt accggtcgta aatgccagcg acgagctggt cggcatcgtg tcgatgcggg
     3481 acgtctcgac ggaaaccaac cagaacgcca acgtcggcaa ggcggtcgag cagatcagcc
     3541 agggaccggc gaacaactag tttggtgggt tgctgggttg gcagcccagc aacccggcaa
     3601 cctcctgcta acctagcgcg atgccgaaac gcgttgcgct tgctctcttt ctcgttgccg
     3661 tcacgccgct ctacgccgcg cgacccgtgg tcaagcctgt gccgaaggtg gcgccggcac
     3721 ccgttggcat cacggtcgat acgcacgccg acacgcccac cgagtttctc gatcacccct
     3781 tcgacctcgg cgccctcaac acccgcgggc acttcgacta tccgcgcatg aaagcgggcg
     3841 gtctcgacgc ggagttcttc gccgcgtacg ttcccgccaa gtacgccaat aagggcgcgg
     3901 ccgcgttctg cctcaagatc atggagacca tccatgagat ggtggacgcc tatccgacct
     3961 gggttcgctt cgcgacgtcg acggccggga tcaaggccgc ggtcgccgac aaccgccgcg
     4021 ccatcctcat cggcatcgaa ggcggccacg ccatcgaaga ctcgctcgat attctccgcg
     4081 cgttctaccg cttcggcgcg cgctacatga ccctgacgca tacgaacacg aacaactggg
     4141 cggactcctc cggcgacgag gcgatccaca acggtctcac gccattcggc aaagaagtcg
     4201 tcctggagat gaaccgcctc ggcatgctcg tcgacatctc gcacgtcagc gacaagacct
     4261 tctacgacgt catcgaagtg tcgaaggcgc ctgtgatcgc ctcgcactcc tcctcgcgcg
     4321 cgctggctaa agcaccgcgc aacatgacgg acgagatgct cgtcgcgctc gcgaggaacg
     4381 gaggcgtggc gatggtgaac ttctatcccg tctttctctc cgacgaggtg gccaaagcgt
     4441 cgaaggagcg ggaggaaagg ctcaagcccg agatcgcggc attgaaggcg aaggatccgt
     4501 cagagggagc cgtctacgaa gaaggtttac gcaagttgat ggcggcgaat ccgttgccga
     4561 aggtgtcgtg gaccgtgatc gtcgatcaca tcgaccacat ggtcaaagtc gccggcatcg
     4621 atcacgtcgg aatcggctcc gacttcgacg gcatccccga agtccccgaa ggaatggagg
     4681 acatcagcaa actccccgcg atccgcaccg agctgaagcg ccgcggctac gcggatgccg
     4741 acgtccgcaa aatcatgggc gagaatttca tgcgcgtgtt cggggaagtg gagaagatcg
     4801 cgaaggagct gcaagcgaag gaacccgcgg cggcgaagta ggggagtttt cgcgcgaagc
     4861 gctttggact ggtctttatc cgcagatgac gcagattgaa cgcagatgaa aatctgtgtc
     4921 catctgcgtc atctgcggac caggccagtc caaagcgatt cgcgcgaaaa ctgccacgcc
     4981 ccctacgacg gcgccgaaaa cgtccgcgcc gggtcatctg cggcgacgta gtcgcgaacg
     5041 tagcgcacga gcgctggcgt ggctcgctcg ccctcgaccg ccgtgcggag gtacgagagc
     5101 gcggtcacgc tggcgagggt gacgccggcc gccgtgaaca gtcctgccag ctcgcgggcg
     5161 atgttcgctg cttcgctcca ttccttgcgg cggaggagct cctcggtgat gtcgaggttg
     5221 acgaagccgg cgtccgaggt catgccgagg gcgcggaagg aggcggctgc ggcgtagaga
     5281 agctcgagcg cccggtcatg ctcacccaac gcgcggagga tcgctgcgac cgtccactct
     5341 gagcggatac gctccgcatc catctgcagt tccgcgaaac cggttgccgc ggcctgcgcc
     5401 tcggccagtg ctttcgccgg ccggccgcgc ctcaggctgc attcggcgag attggcgcgg
     5461 acgcgggcca gttccatgcg gtcatcgaaa cgtttgagca tcggcacgac ctcgagccac
     5521 gtttgctcgg cacggacgac atccccctgt tcgaagcgga tggcggcatc gagcatcagc
     5581 gttttcgcca gcggcatcgt gtctccgaat tcgcgcaaaa gctcccttgc ggactcgagg
     5641 acagacagcg cctccccgta cagcgtcatt ttgaaaagcg tgccggcgcg ggtgtagagg
     5701 gcctggccga tgtcgaagtc gccgaggggg agcaatcggt agaaggcggc agcgaggtcc
     5761 gccgcttcga gcgactccga atatctgccg agatgacgaa gcgcattcgc gcgttgtttc
     5821 cagcagtcgc ccgcgcaaac gtggatcgcc ggaatgtcga gcgagccggt gacagtctcc
     5881 gcgacgttga tcagcagcaa cgcgtgctgc ggccgccgtt tgacctcgat ctccacctcg
     5941 gtcagcaagc gaaggaccat cgcctcgctg cgttggtggg gattttccgc gaacgcggat
     6001 aaccattcct cgatcggcag gcgcatcagc tcgccgaaga atgcttcggc ccgcgccgcc
     6061 tccgctccca tcctctgccg cgtggcgagg atgtcgaatt gctccaacgg ccgttcgcga
     6121 tcgctttttc gtgactcgat gaagtcgagc acgttgtcgt gcagacgaaa cgtcgccgcg
     6181 agcgcgtcca gttcgttccg gcaatcggcg cagtcggcga gatgcgtcgt gattgccaga
     6241 cggtcgccca caaggtcgcc ttcgaggtac ttcgccagcg tcgtccgatc gggatgctcg
     6301 ttcatgagag ctccccaagc gcacgcctcg ctgctgccag acagcggtgc accttcagcc
     6361 ggatgtatcc cggcgacgtt ccgtagcgcg tggcaatctc ccgcggattc aatccttcga
     6421 cgtagcg
//
LOCUS       DATLVW010000347         3245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_58624, whole genome shotgun sequence.
ACCESSION   DATLVW010000347 DATLVW010000000
VERSION     DATLVW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58624"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(982..1623)
                     /locus_tag="VNN25_10555"
     CDS             complement(982..1623)
                     /locus_tag="VNN25_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92010.1"
                     /translation="MPVPTLDFGVFIAYLLPGVIVLYGATFVVPQMREWLQPGAGRLG
                     IGGAVIVTIVALTAGRLISIGRVVLIESTFTTPLPLLNCAERPSLGGIPPLSLDYRQV
                     FDSGRRDAFILAVANEQRPYQFCGNTAMAVVLCLVCWLVTLPKHTLRRSRVLLIAVAA
                     ISVTIVLYLGARSSYYGYMRAMAAINGVQFASLDHLGKPCQAIAVSSESRRSP"
     gene            1626..2651
                     /gene="rapZ"
                     /locus_tag="VNN25_10560"
     CDS             1626..2651
                     /gene="rapZ"
                     /locus_tag="VNN25_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXH92011.1"
                     /translation="MKNVRFKGEKRQITETLVLVQACRACEKIPIDRSEIVTCRSEIA
                     TFGQDKLSNMPRPEYLVIITGLSGSGKSYVQSTLEDVGFYCCDNLPIELIEPFLEEVG
                     AHENANRVGIVVDVRTHDFANVFPTFYRERIQKLVPHVVLIFLDASDEVIARRYSETR
                     RPHPLAQDQPLVQAIAAERAALTEVKSLANMVLDTSQFSVHELKAEVMRRFELKDEKV
                     TMLVTIVTFGFKYAMPYNVDLLFDVRFLPNPHFVEELRPKTGLDPEVVAYLKAQPGYD
                     IFYNKLIDFVDYLLPQYQKEMKSYLTIGIGCTGGKHRSVAIGQRLGDDLAARGYAVEI
                     VHRDCKR"
     gene            2898..>3245
                     /locus_tag="VNN25_10565"
     CDS             2898..>3245
                     /locus_tag="VNN25_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXH92012.1"
                     /translation="MDELLDMYDRKMSNFAEGDIIRGKVLKVSGAEVVVDIGYKSEGV
                     LPTHEVTGFDGQITVKAGDELDVFIERLENNSGYIVLSREKAERMLVWDRIEAAFKAD
                     EPITGRVVERVKGG"
BASE COUNT          707 a          942 c          963 g          633 t
ORIGIN      
        1 taggccaacc gagattgtgt catcctgaga ctgccggagc cgcccgcgtg aatcgcgatg
       61 tttgcgcagt aggtgctcgg cggcgtagga cgtcgaagga tctcaaactt cgcagatcgt
      121 gttcgtccag cgcgagcgat cgtgatccaa gacgctcaaa cgagatcctc gcattttgag
      181 atccttcgac cgtcctacgc cgccgtttct ggcgggggtt agcccgcatt ttctcacact
      241 ctcactggaa tttaatgaca tccgacgtca tagcgaacgt ttgagcgtca gatattcgat
      301 gctacgaact ccgcctcgcg aagtaaccgc gtcatcgcga acgtttgacc gtcagacatt
      361 cgcgggctcc gccgacattg cgaatgtctg acgctcagat attcatgtcc agcggcgatc
      421 tcttgaatga cttccgcgtc atcgcgaacg tctgagcgtc agatattcga ttctacgaaa
      481 ttgtctcgcg aggtgatccg cgtcatcgcg aacgtctggc cgtcagacat tcgcggggtc
      541 cgtcgacatt gcgaatgtct gacgctcaga tattcatgtc cagcggccag ctcttgaatg
      601 acttccgtgt tgcagcgtac gtcggagcgt cagatattcg acgctgcgaa gtctcgccga
      661 gtaaccgcga ccgtctcgag caccgttcgg gccaggcctt cgctcgtttg tcgactgcaa
      721 atcattgaga aacggccact tctggcggct catgcgagac tctcgcgacg gagctcagtt
      781 attcggatca caagtggtcg aaaactggaa ggttgccggg cttctcgcgt gcagcacctg
      841 gtaagtcccc cctcatccgg cttcgccacc ttctcccccc gcaaaaagcg cggggggaga
      901 aggcgctcga ttggagagct gggagccggg ggcgtgagaa atgcgggcta aggatgacac
      961 cacgcgtttt gcgggtatcg ttcaagggct tcggcggctc tcggatgaaa cagcaatcgc
     1021 ctggcaaggc ttaccgaggt gatcgagcga cgcaaattgc acaccgttga tggccgccat
     1081 ggcacgcatg tatccgtagt acgaggagcg cgcgccgagg tacaggacga tcgttaccga
     1141 gatcgcggcg acggcgatga gcaatacgcg tgaacggcga agcgtgtgct tcggcaaggt
     1201 gaccagccaa cacaccaggc acaggaccac ggccattgct gtattgccac agaattgata
     1261 tggccgttgc tcgttggcaa ccgcgagaat gaatgcgtcg cgccggccgc tgtcgaacac
     1321 ttgccggtaa tcgagactga gcggtggaat accgcccaga gacggtctct cggcacagtt
     1381 cagaagcggc aacggcgtgg tgaaggtgct ttcgatcagc acaacgcggc cgatgctgat
     1441 gagcctacct gcggtgagtg cgacgatggt cacaatcacg gcaccaccga ttccgaggcg
     1501 gccagcgccc ggctggagcc actcgcgcat ctggggcact acgaacgttg caccgtacag
     1561 cacgatcacg ccgggcagca gatacgcgat aaagacgccg aaatcgaggg ttgggaccgg
     1621 cattcatgaa aaatgtacgc ttcaagggtg agaagcgtca aatcacggag acgctcgtcc
     1681 tcgtccaagc ttgccgggca tgtgaaaaga tcccgatcga tcgttcagag atcgtgacct
     1741 gccgctccga gatcgcgacc ttcgggcagg ataaactgag caacatgcct cgccccgagt
     1801 acctcgtcat catcaccggc ctctccggtt cggggaagag ttacgtgcag tcgacgctgg
     1861 aagatgtcgg gttttactgt tgcgacaacc tgcccatcga gctgatcgag ccgtttctcg
     1921 aggaggtggg ggcgcacgag aacgcaaatc gggtcgggat cgtcgtcgac gtgcgcacgc
     1981 acgatttcgc caatgtcttt ccgacgttct accgcgaacg gatccagaag ctcgtcccgc
     2041 acgtggtcct gatctttctg gacgcgagcg atgaagtaat cgcgcgccgc tattcggaga
     2101 cgcggcggcc gcatccgctg gcgcaggacc agccgctggt ccaggcaatc gccgcggaaa
     2161 gggctgcgct gaccgaggtg aagtcgctcg cgaacatggt gctggatact tcgcaattct
     2221 cggtgcacga gctcaaggcc gaggtcatgc gccggttcga gctgaaggac gagaaagtga
     2281 ccatgctcgt cacgatcgtc accttcggct tcaagtacgc catgccctac aacgtcgacc
     2341 tgttgttcga cgttcgtttc ctgccgaacc ctcactttgt cgaagagctg cggccgaaaa
     2401 ccgggctcga tcccgaggtc gtcgcttacc tgaaggcgca gccgggatac gacattttct
     2461 acaacaaact catcgatttc gtcgattacc tcctccccca ataccagaag gaaatgaaga
     2521 gctacctgac gatcggcatc ggctgcaccg gcggaaagca ccgctccgtt gccatcggac
     2581 agcggctcgg cgatgatctg gccgcccgcg gatacgcagt cgagatcgtg catcgcgact
     2641 gcaagcggtg aggctggcga gaggaagtct gtccatcgtg atcgccgcgc cttccgtaat
     2701 tccttgactg atcgagagct acgccactaa actccgcatt tcgactacta tcaacaattt
     2761 acgcacgatg cgccttcggc cgaccgcgcc ggaaattcca ggaggtatcc cgtttcatgc
     2821 cgtcgagtga cgatcctaaa aacagtaaac aaccgctccg aaaagggggg gagacaaacg
     2881 accttgaagg catgggcatg gacgagctac tcgacatgta cgaccggaaa atgtcgaatt
     2941 tcgcagaggg cgacatcatc cgcggcaagg ttctcaaggt gtccggcgct gaagtggtcg
     3001 tcgacatcgg ttacaagtcc gagggcgtac ttccaacgca cgaggtaacc ggcttcgacg
     3061 gtcaaatcac cgtcaaggcg ggcgatgagc tcgacgtctt catcgaacgc ctcgagaaca
     3121 acagcggcta catcgtgtta tcccgtgaga aggcggaacg gatgctggtc tgggaccgaa
     3181 tcgaagcagc gttcaaggcc gacgagccca tcaccgggcg tgtcgtcgag cgtgtcaaag
     3241 gcgga
//
LOCUS       DATLVW010000348         4228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_592740, whole genome shotgun sequence.
ACCESSION   DATLVW010000348 DATLVW010000000
VERSION     DATLVW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592740"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /gene="purL"
                     /locus_tag="VNN25_10570"
     CDS             <1..1744
                     /gene="purL"
                     /locus_tag="VNN25_10570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXH92013.1"
                     /translation="LVNAFTLGIAPADRIFRAKAAGPGNPVIYVGSRTGRDGIHGASL
                     LASASLDALAEEKRPAVQVGDPFTEKLLLEACLEIMESDAVVGIQDMGAAGLTSSSFE
                     MAGRAGTGVRMHLDRVPVRESGMTPYEIMLSESPERMLIVAKRGREDEVVRTFEKWDL
                     NAAVVGEVTDDGFVRLFWSGEEVAAIPVDPISTEAPVYERPMSKPERSLPAILDRQRV
                     EDGDLTDELFHLIASPNLCSKHWIWEQYDTTVRTNTIAGPERRDAAIVRVKGTGRALA
                     MTSDVNPLYCFLDPYEGGKQAVAEAARNIAVSGGRPIAITDCLNFGSPERPEIMGQFA
                     ECIRGISDACIAFNTPVVSGNVSFYNETEGRAVLPTPTVGMVGILERETDGCGIAFPE
                     AGLDIVLIGDTRDELGGSEWQLMRLPEGLAQSPRVDLSQEKALVAFFIEAHEHRLLRS
                     AHDLSNGGLAVALAECAMTGSGCHVDLGSHADAIDAIALLFGESQGRAIVSTSHTDEL
                     LRLAKHHALPTMHIGRTEIASFLIERNGVPLVRTSTPELARIWRTSFALLLGGDSVDD
                     VIRGVGEEAELIAR"
     gene            complement(1756..2310)
                     /locus_tag="VNN25_10575"
     CDS             complement(1756..2310)
                     /locus_tag="VNN25_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXH92014.1"
                     /translation="MSAVESLRARLPENARDTKLNLQSVLQGGTLSAAQRWGVAVASA
                     AAARNRELLDAILADARDEAGDAVIDDALASASLMAMNNVYYRFKHMIAKESYEQKPA
                     RLRMQRIAKPATNKPDFELFCLAVSAINGCEMCIRSHEAAVISGGLTEDHVVDAVRIA
                     AVVNAAAVALELPVFAAEAAILSS"
     gene            complement(2316..2852)
                     /locus_tag="VNN25_10580"
     CDS             complement(2316..2852)
                     /locus_tag="VNN25_10580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXH92015.1"
                     /translation="MLTVGDKFPDYKLTTSVGTEKGKEFGSVSTKDNQGKWLVFYAYP
                     KDFTFICPTEIVEFDKKGSEFSDRDAVVLGGSTDNEYSHLAWRVAHPDLKNVKTPLVF
                     ITPAFAAQLGILHPEAGAALRVTYIVDPNGIIRWVNANDLSVGRNVDEVIRALDALQS
                     DELCPCNWRPGQQTLGAA"
     gene            complement(2928..3338)
                     /locus_tag="VNN25_10585"
     CDS             complement(2928..3338)
                     /locus_tag="VNN25_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXH92016.1"
                     /translation="MRNSVTILQEHGIQPSAQRVAVADYVLQTLEHPSADVVWTRVKA
                     RVPYISRATVYNTLNLFVEKGLLQALALAEDSVVFDPKMERHHHFVDEESGTIHDVPW
                     DKVQVCNIESLKGYEIHDYQVVMRGIRKGARRKA"
     gene            3418..3732
                     /locus_tag="VNN25_10590"
     CDS             3418..3732
                     /locus_tag="VNN25_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92017.1"
                     /translation="MRSAQVSELKERLDEVIEAVRNGETVEIRDGEKAVAEFVPSVAK
                     PILPEMSPDQKALEANLDELVRQGKARRGTGTLPDDFFTRPLPESRKSVLDALLEDRK
                     SD"
     gene            3732..4154
                     /locus_tag="VNN25_10595"
     CDS             3732..4154
                     /locus_tag="VNN25_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH92018.1"
                     /translation="MRFWDSSALIPLLIREKSSDAMRTILTSDRHIAASVITPIEIES
                     ALWRRRHHGDLDAEQHAASEETFAELTRSWSEIGDIILARGIALDLLSRHVLRAADAI
                     QLATAIVAAGSSRVPITFVTLDLDLANAARAEGFEVLS"
BASE COUNT          790 a         1264 c         1361 g          813 t
ORIGIN      
        1 cctggtgaac gcgttcacgc tcggcatcgc accggcggat cggatcttcc gcgccaaggc
       61 ggcggggccg ggcaaccccg tcatctacgt cggctcgcgc acgggccgtg acggcatcca
      121 cggggccagc ctgctcgcgt cggcgtcgct cgacgcgctc gccgaggaga agcggcccgc
      181 ggtgcaggtg ggcgatccgt tcacggagaa gctgctgctc gaagcgtgtc tggagatcat
      241 ggagtcggac gccgtcgtcg gcattcagga catgggcgcg gccggcctaa cgtcgtcatc
      301 gttcgagatg gcgggccgcg ccggtaccgg cgtgcgcatg catctcgacc gcgtgccggt
      361 gcgcgagagc ggcatgacgc cgtacgagat catgctgagc gagtcgccgg aacgaatgct
      421 catcgtggcg aaacgcggcc gcgaagacga ggtcgtgcgg acgttcgaga agtgggatct
      481 gaacgctgcc gtcgtcggcg aagtgacgga cgacggattc gtgcggctct tctggagcgg
      541 cgaagaagtg gctgcgattc ctgtcgatcc gatatcgacg gaggcgccgg tgtacgagcg
      601 tcccatgtcg aagccggaac ggtcgttgcc ggcgattctc gatcggcagc gagtcgaaga
      661 cggcgacctc acggatgaac tgtttcatct gatcgcgtcg cccaatcttt gctcgaagca
      721 ctggatctgg gagcagtacg acacaaccgt gcgcacgaac acgatcgcag gaccggagcg
      781 tcgcgatgct gcgatcgtgc gcgtgaaagg caccggccgc gcgctggcga tgaccagcga
      841 tgtcaatccg ctttattgct ttctcgatcc gtacgaagga ggcaagcagg ccgtggccga
      901 ggcggcgcgg aacatcgcgg taagcggcgg ccggccgatc gcgatcaccg actgcctcaa
      961 cttcggctca ccggaacggc cggagatcat gggtcagttc gccgagtgca tccgtggcat
     1021 cagcgacgcg tgcattgcct tcaacacgcc ggtggtctcc ggaaacgtct cgttctacaa
     1081 cgagaccgag ggacgggcag tattgccgac gccgacggtg ggcatggtcg gaatcctcga
     1141 gcgcgagacg gacgggtgcg ggatcgcgtt tcccgaggct ggactcgaca tagtcctgat
     1201 tggcgacact cgcgatgagt tgggcggatc ggagtggcag ctgatgcgcc ttcccgaggg
     1261 cttggcgcaa tcgccgcgcg tggacctctc tcaggaaaag gcactcgtcg cgttctttat
     1321 cgaggcacac gagcaccggc ttctgcgcag cgcgcacgat ctttctaacg gcggacttgc
     1381 ggttgcgtta gccgagtgcg cgatgacggg cagcggatgc cacgtcgatc tcggcagcca
     1441 cgcggacgca atcgacgcaa tcgcgctcct cttcggcgaa tcgcaggggc gggcgatcgt
     1501 gtctacgtcg cacaccgacg agcttctccg tctcgccaaa catcatgctc tgcccacgat
     1561 gcacatcggc cgaacggaaa ttgcgagctt cctcatcgaa cgaaacggcg tcccgctcgt
     1621 ccgcacatcc acgcccgagc tcgcccgcat ctggcgcaca tccttcgccc tgctcctcgg
     1681 cggcgacagc gtcgacgatg tcattcgggg agtgggcgaa gaggcagagt tgatcgcgcg
     1741 gtgatgaggt ccgtatcagc ttgaaagaat tgccgcttct gccgcgaaaa ctggcaactc
     1801 aagcgccacc gctgccgcat tcacgaccgc ggcgatacgc acagcatcga caacgtgatc
     1861 ctcggtgagc ccgcccgaga tgacggctgc ttcgtgcgag cggatgcaca tctcgcagcc
     1921 gttgatggcg ctcactgcga ggcagaagag ctcgaagtcg ggcttgttcg tagccggttt
     1981 tgcaattcgc tgcatgcgaa gacgcgcggg cttttgctcg tacgactcct tcgcgatcat
     2041 gtgcttgaag cggtagtaga cgttgttcat cgccatcagc gaggccgagg cgagggcatc
     2101 gtcaataacg gcgtcgccgg cttcgtcgcg ggcgtcagcg aggatggcgt cgagcagctc
     2161 gcggttgcga gcggcggccg ccgaagcgac cgccaccccc caccgctgcg ccgccgagag
     2221 cgtgccgcct tgcagcacgc tctgcagatt gagcttggtg tcgcgcgcgt tctcgggcag
     2281 gcgcgcgcgc agcgattcga cggcgctcat gatgtttacg ccgccccgag cgtctgctgt
     2341 cccggacgcc agttgcacgg gcagagctca tcgctctgca gcgcgtcgag cgcgcggatc
     2401 acttcatcga cattgcggcc gacggagaga tcgttggcgt tgacccagcg gatgattccg
     2461 ttcggatcga cgatgtacgt cacgcgaagt gcggcgccgg cttccggatg aaggatgccg
     2521 agctgcgctg cgaacgccgg cgtgatgaag accagcggcg tcttcacgtt cttcagatcg
     2581 ggatgggcga cgcgccaggc gaggtgcgag tactcgttgt cggtgctgcc gccgaggacg
     2641 acggcgtcac gatcgctgaa ctcggagccc ttcttatcga actcgacgat ctcggtcggg
     2701 cagatgaagg tgaaatcttt cgggtaggca tagaaaacga gccatttccc ttggttgtct
     2761 ttcgtgctga cgcttccgaa ctcttttcct ttttcggttc cgaccgatgt ggtcagtttg
     2821 taatcgggga acttgtcacc aacggtaagc atgggcatgt gtctttctcc ttttgatttg
     2881 gacgatgtcg cgatctggac accgtctagc gagttgccaa aattttttca ggccttacgc
     2941 cgggcaccct tgcggatgcc gcgcatgacg acctggtagt cgtgaatctc gtagcccttc
     3001 aatgattcga tgttgcagac ctggactttg tcccagggca cgtcgtggat cgtaccgctt
     3061 tcctcatcga cgaagtggtg atgccgctcc atcttcggat cgaagacgac gctgtcttcc
     3121 gcgagcgcga gcgcctgcag caaccccttc tccacgaaga ggttgagggt gttgtagacg
     3181 gtggcgcggg agatgtacgg cacgcgcgcc ttcacgcgtg tccagaccac gtccgccgaa
     3241 ggatgctcga gggtttgcag gacgtagtca gcgacggcca cgcgctgcgc cgaaggctgg
     3301 attccatgct cctgaagaat ggtcacgctg ttacgcacga tgattagatt aaatctaaag
     3361 atggacaatg tcaagcgcat cgcaacccgt ctcctgccga ggtacactga ttgcgccatg
     3421 agaagcgcac aggtaagtga gctgaaggag cggctggacg aagtcatcga agcggtgagg
     3481 aacggggaga cggtggagat ccgggacggc gaaaaggcag tggccgagtt tgttccgtcc
     3541 gttgccaaac caattctgcc ggagatgagc cccgatcaga aagcgctcga ggcgaacctg
     3601 gacgagcttg tccgtcaagg gaaggccaga agaggaacag gcactcttcc ggatgatttt
     3661 ttcacacgac cgctgcccga aagcaggaag agcgttctcg acgctcttct cgaagatcgc
     3721 aaatccgact gatgcgtttc tgggactcct ccgcgctgat tccgctcctt attcgagaga
     3781 agtcgtcgga tgccatgcgc acgatcctga ccagcgaccg gcatatcgct gcatcggtga
     3841 tcaccccaat cgagatcgag agtgcacttt ggcgccgccg tcatcatggc gatctcgatg
     3901 cggaacaaca cgctgcttct gaggagacct ttgccgaatt gacccgcagt tggtccgaga
     3961 tcggcgacat catcctcgcc cgagggattg cgcttgacct gctttcgcgg cacgtcctcc
     4021 gcgctgctga cgcgatccag cttgcgactg caatcgttgc agcaggcagc agtcgcgtcc
     4081 ctatcacctt cgtcacactc gatctcgacc tcgcgaatgc cgcccgcgcc gaaggatttg
     4141 aggtcctgtc gtgagcaccg tttgcgcgtc actgctgcgc gacgttataa gtaaagtcgt
     4201 tgccatgaga agagcacagg tgagcgaa
//
LOCUS       DATLVW010000349         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_264080, whole genome shotgun sequence.
ACCESSION   DATLVW010000349 DATLVW010000000
VERSION     DATLVW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264080"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..197
                     /locus_tag="VNN25_10600"
     CDS             6..197
                     /locus_tag="VNN25_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HXH92019.1"
                     /translation="MRTTINLDEQLIADAQRMTGIQERTALIHEGLRALITRESSRRL
                     ARLGGSDPGAKAPRRRRVS"
     gene            194..562
                     /locus_tag="VNN25_10605"
     CDS             194..562
                     /locus_tag="VNN25_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH92020.1"
                     /translation="MIIVDTSVWIDHLHKAVPLLGDTLENGDVATHPFVIGEIACGEI
                     ARRQEVLTLLSTLPSAVVATDEEALHFIEHYRLMGKGIGYIDAHLLASAMLTNGATLW
                     TRDKRLNAIATKLQIEFASA"
     gene            complement(717..1061)
                     /locus_tag="VNN25_10610"
     CDS             complement(717..1061)
                     /locus_tag="VNN25_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92021.1"
                     /translation="MSEVPTSEVPSPAADTPTVPAPPTFNVLAMFGGLVLSVVLGAIG
                     NVIAGFASADVGAKIFGFLLGIVPGVIFVGLAALLRRKTTAFATGLLIGGCLVGLIGG
                     ICGASMVGFTVR"
     gene            complement(1061..2467)
                     /locus_tag="VNN25_10615"
     CDS             complement(1061..2467)
                     /locus_tag="VNN25_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH92022.1"
                     /translation="MTTATRPYLFYDAVVSICTTCFRKIDGKIVFEEGRVWMLKHCPT
                     HGSERVLMSDDVDYYRQCREIYLKPPEMPARRNTPIRWSCPYDCGLCPDHEQHSCISL
                     IEVTDACNLRCPICYAESGPERQHHRSLAHIEAMLDAVVENEGEPDVVQISGGEPTIH
                     PDFFAILDAAKRRPIKHLMVNTNGVRIARDAAFVERLATYKPLFEIYLQFDSLRPRPL
                     EILRGADLREIRMKALERLNALDISTTLVVTLQRGVNDDEIGEIVDFALTQPCVRGVT
                     LQPVQVAGRLDGYNDGENRLTLSEVRRKVLEQSTLFAPEDLIPVPCHPDSLCMGYAFK
                     GGGEVVPLTSLVGREAFIDGSRNTIVFERDPAARGAFLKLFSTSLGAESQPQKLKDLL
                     CCLPRVQGDVSYGNLFRLLIVEFIDAQSFDLRSIKKTCIHIVHPDGRLIPFDTYNLFY
                     RDELETTRLGPLREWAVR"
     gene            complement(2391..>2731)
                     /locus_tag="VNN25_10620"
     CDS             complement(2391..>2731)
                     /locus_tag="VNN25_10620"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HXH92023.1"
                     /translation="RHPVALYEIAFLLALLPVLRRIRKNEVAGTGPLSRDGDTFRIFL
                     ASYLLFRLAVDFLKPDPRPILLGMTAIQWACAAGLLYYLSVLCNDNRDPALPFLRRGR
                     LDLHDVLPQD"
BASE COUNT          563 a          811 c          885 g          472 t
ORIGIN      
        1 acgttatgcg cacgaccatc aatctcgacg aacagttgat cgcagatgcc cagcggatga
       61 cgggcatcca ggaaaggacg gccttgattc acgaaggact acgggcgcta atcaccagag
      121 aaagctcccg ccgcctggcc cggctcggtg gaagcgaccc aggcgcgaaa gcacccagaa
      181 ggcgccgcgt ttcgtgatca tcgtcgacac ttccgtgtgg atcgatcatc tccacaaggc
      241 ggtacctctg ctcggggata ctctcgaaaa tggagacgtg gcaacgcacc cgttcgtcat
      301 cggcgagatt gcctgcggcg agattgcaag gcggcaggaa gtgctcactc ttctctcgac
      361 ccttccttct gcggtggtgg ccaccgacga ggaagcgctt cacttcatcg aacactatcg
      421 cctgatggga aagggaatcg ggtacatcga cgcacacctt ctcgcttccg cgatgctcac
      481 gaacggtgca acgctctgga cacgcgacaa aagactgaac gcgattgcaa ccaagctgca
      541 gatcgaattc gcgagcgctt gatcaaccgc cgaggaggcg acacgtgaag tcaggtgggt
      601 aagcgcggat tcaaaccgta attgcaacca cagagacaca gagatcacag agttgcaata
      661 acgatctcag tgatctctgt gcctctgtgg ttcatgcatc ttccagacgg ggcgattcaa
      721 cgaacggtga accccaccat cgacgccccg cagatcccac cgatcaggcc gacgaggcag
      781 ccgccgatta ggaggccggt ggcgaaggcg gtggttttgc ggcggaggag cgcggccagt
      841 ccgacgaaga tcacgccggg gacgatgccc agcaggaatc caaagatctt cgcgccgacg
      901 tcggcgctcg cgaatccggc gatgacgttc ccgatcgcac ccagcacgac ggaaagcacc
      961 aatccgccga acatggccag gacgttgaag gttgggggag ccggtacagt tggggtgtcc
     1021 gccgcgggag agggaacttc actcgtcggt acttcgctca tcatcgcacc gcccattctc
     1081 gcagcggccc gagacgggtg gtctcgagct cgtcgcggta aaagagattg taggtatcga
     1141 acgggatcag ccgtccgtcg ggatgcacga tgtggatgca cgtcttcttg atcgagcgga
     1201 gatcgaacga ctgcgcgtcg atgaactcca cgatcagcag gcggaagaga ttgccgtagc
     1261 tgacatcgcc ctgaacgcgg ggcaggcagc agaggagatc cttcagcttc tgcggttgtg
     1321 actcggcacc gagtgaggtc gagaaaagtt tgaggaacgc gccgcgcgcg gcgggatcgc
     1381 gctcgaagac gatcgtgttg cggctgccgt cgataaaagc ctcgcgcccg accagcgacg
     1441 tcagcggcac gacctcgccg ccgcctttga acgcgtagcc catgcagagc gaatcgggat
     1501 ggcaggggac ggggatgaga tcctccggcg cgaatagcgt cgactgctcc agcaccttcc
     1561 ttcgcacctc ggacagcgtg agccggttct cgccgtcgtt gtaaccatcg agccgtccgg
     1621 cgacctgcac cggctgcagc gtcacgccgc ggacgcatgg ctgtgtcagc gcgaagtcga
     1681 cgatctcgcc gatctcatcg tcgttgacgc cgcgttgtag cgtgacgacc agggtcgtcg
     1741 agatgtcgag cgcgttcagc ctctccagcg ccttcatccg gatctcgcgc aagtcggcgc
     1801 cgcgcaggat ctccagcggc cgcgggcgca gcgagtcgaa ctgcagatag atctcgaaga
     1861 gcggtttgta cgtcgccagg cgctccacga aggccgcgtc gcgagcgatg cgtacgccgt
     1921 tcgtgttgac catgaggtgc ttgatcggac gccgtttcgc ggcgtcgagg atcgcgaaga
     1981 agtcgggatg gatcgtcggc tcgccgccgg agatctgcac gacatccggc tctccctcgt
     2041 tctcgacgac ggcatcgagc atcgcttcga tatgcgcgag cgagcggtga tgctgccgct
     2101 ccggtccgct ctccgcgtag cagatcgggc agcgcaggtt gcaggcgtcg gtgacttcga
     2161 tcagcgagat gcaactgtgc tgttcgtgat cggggcagag gccgcagtcg tacgggcacg
     2221 accagcgaat cggcgtattg cgccgcgccg gcatctccgg tggcttgagg tagatctcgc
     2281 ggcactgacg gtagtagtcg acatcgtccg acatcagcac gcgctccgac ccgtgcgtcg
     2341 ggcagtgctt gagcatccag acgcgccctt cctcgaagac gatcttgccg tcaatcttgc
     2401 ggaagcacgt cgtgcagatc gagacgaccg cgtcgtagaa aaggtagggc cgggtcgcgg
     2461 ttgtcattgc agagtacgct gagatagtag agcaatccgg cggcacatgc ccattgaatt
     2521 gcggtcatgc cgaggaggat ggggcgcgga tcgggcttga gaaagtcgac cgcaagacga
     2581 aagaggagat acgaagccag gaagattcgg aaggtatcgc cgtcgcgcga caacggacct
     2641 gtgccggcaa cttcgttctt cctgatgcga cgcagcactg gcaggagcgc gaggaggaac
     2701 gcgatctcgt acagcgccac gggatggcgc g
//
LOCUS       DATLVW010000350         4912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_182939, whole genome shotgun sequence.
ACCESSION   DATLVW010000350 DATLVW010000000
VERSION     DATLVW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182939"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1277)
                     /locus_tag="VNN25_10625"
     CDS             complement(213..1277)
                     /locus_tag="VNN25_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HXH92024.1"
                     /translation="MPHLFDPYAIRSVTFRNRVFVSPMCQYSSEDGLPNDWHFVHLGS
                     RAVAGAGLVMVEASGVSPTGRITAWDSGFWSQEHADAFERIVEFIEGNGAVAGIQLAH
                     AGRKASTDVPWRGGAVIREGPASWQTVAPSAIPFRESDPVPLALTGTEIDAIVDQFEN
                     ATRLAVVAGFHVVELHMAHGYLLHEFLSPLSNVRTDEYGGSIENRMRFPLRVAERVRQ
                     AWPDDRPLFVRISASDWVEGGWDLAESLVFAKRLRDLGVDLIDCSSGGAVPGAKIILG
                     PGYQVPFARAIRAEAGVATAAVGLITEAHQADEIVASGSADAVMLARELLRNPYWPLH
                     AARALGAEVKWPPQYERAKQ"
     gene            1416..2150
                     /locus_tag="VNN25_10630"
     CDS             1416..2150
                     /locus_tag="VNN25_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92025.1"
                     /translation="MTLVSRISACLLIALATIPAGAQASRRRSVSPANNLGPTTLVLF
                     TVTDASNGVAVGAATVSFAGQNQVTNSNGQTSLNLPIGKPAVVLVAHAAFAPFLQSIT
                     AQAGGTYELKLTEKPSVTIKTKTNETHIVDIGTAQFAFAATVFSNPERSDIGNFCKED
                     GSDFAPDKTAFTRIVGPAVPATATQCCPSGNVMSANVEMKTGDKFKVYFKDSCGGAEV
                     DFVGREKATGLYQYFRFTDIAEIDFP"
     gene            2194..3594
                     /locus_tag="VNN25_10635"
     CDS             2194..3594
                     /locus_tag="VNN25_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXH92026.1"
                     /translation="MVSIDPRFAIAAVSDEIDRVYAKQREHRDAVAATTAVERIAKLR
                     RLHDAIFAHREDIYAALWADYEKPPSEVDLSEIYPAVSEARHAMRHLRRWMRPRRVRT
                     PLALLGSRSRIVYEPKGVVLIISPWNFPVNLTFGPLVSAIAAGNCVVIKPSEMTPHTS
                     ACMKQILSGIFDESEVAVIEGDSAVAGKLLLKKWDHIFFTGSPAVGRVVMRAAAEHLT
                     PVTLELGGKSPVIVDRTANLDEAARKIAWGKFFNSGQICIAPDYLLVDEAIREPFIEK
                     LRAAIAPETGLIVNDRHAARVKKLFDDAVAGGAKVVAGGEFKQRALGPTMLSNVDPES
                     PLMQQEIFGPLLPMITYRTIDDALEVIAAHEKPLVLYLFSRSRNVIRKVLSGTTAGGT
                     AINDTLLHFFQLNLPFGGVGESGVGKAHGQFGFEAFSNARGVFSQPTRFSTMQLLYPP
                     YTKFKKKLIDLTIKYF"
     gene            3766..4005
                     /locus_tag="VNN25_10640"
     CDS             3766..4005
                     /locus_tag="VNN25_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXH92027.1"
                     /translation="MNFKRIGAYRLFFTSNDLDEPAHIHVERDGMVAKFWLSPISLSK
                     SARFAAHELSKMERIVRENHSDIMEAWHEFLRRRN"
     gene            3980..4246
                     /locus_tag="VNN25_10645"
     CDS             3980..4246
                     /locus_tag="VNN25_10645"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXH92028.1"
                     /translation="MSSFAVETNPRAVEISVTADELVVHLADGRTVTVPLVWFPRLLH
                     ASAHERSDFRLIGEGEYISWPSLDEDLSVSGLLRGTRAVLSSEA"
     gene            complement(4433..4801)
                     /locus_tag="VNN25_10650"
     CDS             complement(4433..4801)
                     /locus_tag="VNN25_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92029.1"
                     /translation="MGTAIAHPDYLAYFNELAGAHPEEIATDSNLDWGQDIQRLNDFV
                     RREKIEHIHVAVVGNWQRFSMPAELLVRGTPATGWIAVSVVELKLGGRNDRGEGYEWL
                     NAYKPVRRIGKSIRVYYIPP"
BASE COUNT          946 a         1505 c         1528 g          933 t
ORIGIN      
        1 atgcgggggt cgggagtcgg gggtcggggg tcggagatgc ggtggtcgag agtcgggggt
       61 cgggggtcgg agatcaggat agtcagagat cagagatcag aagttcgacg cgagaagtct
      121 agtcagaagt ggggaagtca gagtcgatcg tcggagcgcg gacggaggcg atgcctgctc
      181 cgacccccga ctcccgacaa acgaccccct attcattgtt tcgcccgttc atattgcggt
      241 ggccatttca cctcggcacc gagcgctcgc gcggcatgga gtggccagta cggattgcgg
      301 agcagttccc gcgccagcat taccgcgtcg gcggagccgc tggcgacgat ctcgtccgcc
      361 tggtgagcct cggtgatcag gccgaccgcc gcggtggcga cgcctgcttc ggcgcggatg
      421 gcacgcgcga atggaacctg atagccgggc ccgaggatga tcttcgcacc gggcacggcg
      481 ccgccgctgg aacaatcgat gaggtcgacg ccgaggtcgc gtagacgctt cgcgaagacg
      541 agcgattcag ccagatccca tccgccttcg acccaatccg aggcggagat gcgaacgaag
      601 agcggccggt catcgggcca cgcctggcgc acgcgctccg ccacgcgaag ggggaagcgc
      661 atccggttct cgatcgagcc gccgtactcg tccgtccgca cgttggaaag cggtgagaga
      721 aactcgtgaa gaagatagcc gtgagccatg tgcagttcga cgacgtgaaa tcccgccact
      781 accgccagcc gggtggcgtt ctcgaactga tcgacgattg cgtcgatctc ggtgccggtc
      841 aacgcgagag gaacaggatc gctctcgcgg aacggaatcg cgctcggagc cactgtctgc
      901 cacgatgccg gaccttcgcg aatcacggcc ccgccgcgcc agggaacgtc ggtcgatgcc
      961 ttcctgcctg cgtgcgcgag ctggatgccg gcgactgcac cattgccttc gatgaattcc
     1021 acgatgcgct cgaacgcatc agcgtgctcc tgcgaccaga aaccgctatc ccaggccgta
     1081 atgcggccgg taggcgagac gccgctcgcc tccaccatga ccagacctgc gcctgcgacg
     1141 gcgcggctgc cgaggtgaac gaaatgccag tcgttgggca ggccgtcctc gctcgagtac
     1201 tgacacatcg gagagacgaa tacgcggttg cggaaggtca cggagcggat cgcgtacggg
     1261 tcgaagaggt gaggcatcgt tctgcggaat cctctcagcg cgggcagtat agctgtgccc
     1321 gtctgtaacg tttgccgagt cgggcgcgta tatccttata gttactatga cgccgcccgc
     1381 ggctcgtccg cggccggccg atcaaaggag atttcatgac tctcgtttcc cgcatttccg
     1441 cctgcttgct catcgcgctg gcaacgattc ccgcaggagc ccaggcttcg cggcgccgct
     1501 ccgtatcgcc ggccaacaac ctgggaccta cgaccctcgt tctcttcacc gtgacggatg
     1561 cttcgaatgg cgttgcggtc ggggcggcca cggtgtcttt cgccggccag aatcaggtta
     1621 cgaacagcaa tggacagaca tcactcaacc tgccgattgg aaagccggcc gtcgtgttgg
     1681 ttgcccatgc cgccttcgcg cccttcctgc agtcgatcac ggcgcaggct ggtggtacct
     1741 atgagttgaa gctcacggag aaaccgtccg taacgatcaa gacgaagacg aatgagacgc
     1801 acattgtcga catcggcacg gcccagtttg cgttcgccgc aacggtcttc agcaatccgg
     1861 aacgctccga catcgggaac ttctgcaaag aggacggatc tgatttcgct cccgacaaga
     1921 ccgcattcac gcgcatcgta ggtccggccg tgccggccac tgccactcag tgttgcccga
     1981 gcgggaacgt gatgtcggcc aatgtagaga tgaagacggg cgacaaattc aaggtctact
     2041 tcaaggatag ttgcggcggc gctgaagtcg atttcgtcgg ccgcgaaaag gcgaccgggc
     2101 tatatcagta cttccgcttc acggatatcg ccgagatcga ctttccgtag tttccgaaca
     2161 cgagcgtcgg tcgatgcgcg gtagacttcc tttgtggtca gcatcgatcc tcgctttgcc
     2221 atcgccgccg tttccgacga gatcgacagg gtctacgcga agcagcggga gcaccgcgat
     2281 gcggtagccg cgacaacggc cgttgagcgg atcgcgaaac ttcgccgcct ccacgacgcg
     2341 atttttgcgc accgcgagga catctacgcc gcgctctggg ccgactacga gaagccgcct
     2401 tccgaggtgg acttgtcgga gatctatccg gcggtctccg aagcgcggca cgcgatgcgt
     2461 catctgcggc gctggatgcg tccccggcgc gtccgcacgc cgctggccct cctcggatcg
     2521 cgctcgcgca tcgtctacga accgaaaggc gtcgtgctga tcatctcgcc gtggaacttc
     2581 ccggtgaacc tgacgttcgg accactcgtt tcggcgatcg ccgccggcaa ctgtgtcgtg
     2641 atcaagccgt ccgaaatgac gccgcacacg agcgcatgca tgaagcagat cctgagcggc
     2701 atcttcgacg agagcgaggt ggcagtgatc gaaggcgact cggccgttgc tggcaaactg
     2761 ctgctgaaaa agtgggatca catcttcttc acgggcagcc ccgcggtcgg ccgggtggtc
     2821 atgcgggccg cggccgagca cctcacgccg gtcacgctgg agttgggcgg caagtcgccg
     2881 gtcatcgtcg accgcacggc caacctggac gaagcggcga ggaagattgc ctggggcaag
     2941 ttcttcaact ccggccagat ctgcatcgct cccgactacc tgctggtcga cgaagccatt
     3001 cgcgaaccat tcatcgagaa gcttcgcgcc gcgattgcgc ccgagaccgg gctgatcgtc
     3061 aacgaccggc acgctgcgcg cgtgaagaag ctgttcgacg atgcggtagc gggcggagcg
     3121 aaagtggtgg ccggcggcga gttcaagcag cgcgctctcg gtccaacaat gctgtcgaat
     3181 gtcgaccccg agtcgccgct gatgcagcag gagatcttcg ggccgcttct tccgatgatt
     3241 acgtaccgca cgatcgacga tgcgcttgaa gtgatcgccg cgcacgaaaa gccgctcgtg
     3301 ctctacctat tcagccgcag ccgcaacgtg atccgcaaag ttctgagcgg cacgaccgcg
     3361 ggtggaactg ccatcaacga cacgctcctc cacttcttcc agttgaacct tccattcggc
     3421 ggagtcggcg agagcggcgt cggcaaagcc cacggccaat tcggattcga agccttctcg
     3481 aacgcccgtg gcgttttctc acaaccgacg cgcttcagca cgatgcagct tctctatccg
     3541 ccgtacacga agttcaagaa gaagctgatc gatctgacga tcaagtactt ctgaggcgct
     3601 ggcgcccgga gcgatccgct aaacttccga catgtcaatc gtgtggatgg tgagggccgg
     3661 aaccggacgt tcgatctcga cggttgagca ctcatctcac ttgtgaaagt ctactggcct
     3721 gcagagtaga gagatgatgg aggtaggctt cctgcaggag aggaattgaa cttcaaacgg
     3781 attggcgcgt accggctgtt tttcacgagt aacgatctcg acgagccggc tcatattcac
     3841 gtggagcgcg acggaatggt ggcgaaattc tggctgtcgc caatctccct gtcgaaatcg
     3901 gcccgttttg ccgcgcacga actgtcgaag atggagcgga ttgtccgcga gaaccattcc
     3961 gatatcatgg aggcttggca tgagttcctt cgccgtcgaa actaatccgc gtgccgtcga
     4021 gatcagcgtc actgctgacg aattggtcgt ccacctcgct gatgggcgca ctgtgaccgt
     4081 tccgcttgtt tggtttccac gtctgcttca cgcgtctgcg catgaacggt cagacttccg
     4141 actcatcgga gaaggtgagt acatcagttg gccctcactt gacgaggatc tgagcgtatc
     4201 ggggttgtta cgcgggacac gggctgtcct ttcatcggaa gcctagcggc gcttggagca
     4261 agtcctttaa ctccggccag atctgcatcg gcaggcgtcg cggaagtcct caccggcggc
     4321 cagttttatt cagccgcgcc ctcaggccgg catgcgggcg actcgatccg gagtcgccgc
     4381 tgatgcagca ggagatcgtc ggacggctgt taccgataat cagcaccttg cgtcacggtg
     4441 ggatgtaata aactcgaatc gattttccga ttctccgcac tggcttgtat gcgttgagcc
     4501 attcgtagcc ctcgccgcgg tcgtttcttc cgccgagctt gagctccacc acgctgacgg
     4561 cgatccagcc ggtggccgga gtgcctcgca ccagaagttc cgccggcatc gaaaagcgct
     4621 gccagttgcc gaccacggca acgtgaatat gctcgatctt ctcgcggcgc acgaaatcgt
     4681 tgaggcgctg aatatcctgg ccccagtcca gattgctatc cgtcgcgatc tcctcgggat
     4741 gcgcgcccgc gagctcattg aagtaggcca gatagtcagg atgagcgatc gccgtcccca
     4801 cgaagtacca gccgagcaga atggcgaggg cccaacggag cgccttgtgt ctccaccact
     4861 tcgctgccgc ttacgctgca cagatggcga gtagcggata cagcggcagg at
//
LOCUS       DATLVW010000351         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_58698, whole genome shotgun sequence.
ACCESSION   DATLVW010000351 DATLVW010000000
VERSION     DATLVW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58698"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VNN25_10655"
     CDS             complement(<1..940)
                     /locus_tag="VNN25_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXH92030.1"
                     /translation="MNRSETASVLVPEASASGVLTAIGNTPLVEVHQFFGAATSASIF
                     AKLELLNPMGSSKDRAAVSMMLAAISAGLVIPGATTVIESTSGNTGVALASVCKFFRL
                     PLICVVDGRMTSQHGNLLRAFGAKIEEVTVPDPLSGELLPARLRRVQELLESHKPSFW
                     PNQYANANNAAAHQITCAEIFQQLGRAPDFIFCATGTCGTIRGCADYIAARGARTQLV
                     AVDAVGSMIFGGPSQRRLIPGLGSAIRPPLIEGVPVERVVHVSEGDAVLGARRLLETE
                     AILGGGSSGAVMSALSKCEDILRQPKTVVAILADSGE"
     gene            complement(1035..1208)
                     /locus_tag="VNN25_10660"
     CDS             complement(1035..1208)
                     /locus_tag="VNN25_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92031.1"
                     /translation="MPQASAADYAKKSDAKAQGKAAKADGKAKGKAMKAQAKAKGKAM
                     KAQGKAKGKQAGK"
     gene            1427..>2960
                     /locus_tag="VNN25_10665"
     CDS             1427..>2960
                     /locus_tag="VNN25_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HXH92032.1"
                     /translation="MTSWSPLSHGQEALWFLWKLVPATPTYNIALPVGVRGALDVRAF
                     RAALQSLSDRHAALRLEFREENGAPMQRPIDRHEVLLDRIDASDWDDAMVGAAVRERA
                     RQPFDLEASAAMRATLFRRSDDHHVLLIVVHHMTSDLWSLIVAMDELRELYAAEKEDR
                     EPALAPRSTTYIDAIRQQRETLEGETGERLWQFWRDELSGDLPALDLPAARLRPSMQS
                     FRGGTITRRMSVSLTPRLKQLAGRERVTLYMLLLAAYEVLLHRYTGQEAFIVGSPTSG
                     RERPELQDVVGDFVNMVPLRADLSASPTFREVLGRVRARVLGTMQHQDYPFSLLIDRL
                     HPARDLSRSPLFQTTFVLQKFHRFQELSRVMLPGDGEAAIPFADLLLEPMPLAQQDGQ
                     FDLNVEMKEDDRGQLVGAWKYAADLFNAPAIERMAAHFETLLESIVAEPNCAIGALPL
                     LDAVESHDAIARGAGDVTELPDKTVCELFEAQSLRRPDAVAVTCGDDIVTYGELHDRV
                     ASL"
BASE COUNT          496 a          962 c          952 g          550 t
ORIGIN      
        1 gctcgcccga gtcggccaga atcgcgacca cggtcttcgg ctggcgaagg atgtcttcgc
       61 acttgctgag cgccgacatg accgcgccgc tcgatccgcc gcccagaatg gcttcggtct
      121 cgagcaaccg gcgggcgccg aggacggcgt caccttcgct gacgtgaacc acacgctcca
      181 ccggcacgcc ttcgatcagc ggcggccgaa tcgcggagcc gaggccgggg atgagccgac
      241 gctgcgacgg tccgccgaag atcatgctgc cgacggcatc gacagcgacg agctgcgtgc
      301 gcgcgccgcg ggcggcgatg tagtccgcgc agccgcggat cgtgccgcac gtgccggtcg
      361 cacagaagat gaagtcgggt gcgcgtccga gctgctgaaa gatctccgcg caggtgatct
      421 ggtgcgccgc cgcgttattg gcgttggcgt actggttcgg ccagaacgac ggtttgtggc
      481 tctcgagcag ttcctgcacg cggcgcagac gcgccgggag cagctcgccg ctcagcggat
      541 ccggtaccgt cacttcctcg atcttcgcgc cgaacgcacg aagaaggttg ccgtgttgcg
      601 aagtcatccg cccgtcgacg acgcagatga gcggaagacg aaaaaacttg cagacgctcg
      661 ccagcgccac gccggtattg ccggaggtcg attcgatgac cgtcgtcgcg ccgggaatga
      721 cgagaccggc gctgatggcc gcaagcatca tcgagacagc ggcgcggtct ttggaactgc
      781 ccatgggatt gagcagctcg agctttgcga agatggaagc gctggtggca gcaccgaaaa
      841 attgatgcac ctccaccaac ggtgtgttgc cgatcgccgt cagtacacct gacgcggacg
      901 cctcgggcac gaggacagac gcagtttccg atcggttcac gttaaggacg gcacgccaat
      961 cggcgagtct cgcagctgcg gtgatcgttt ccgaagaccg aggcggcgga ctcgccgagg
     1021 aagcgtctcc gggattactt accggcttgc tttcctttgg ctttgccctg ggccttcatg
     1081 gctttgcctt tggctttggc ctgagccttc atggcctttc ctttggcttt gccatcggcc
     1141 ttcgcggcct tcccctgagc tttggcatct gacttcttgg cgtaatcggc tgcacttgct
     1201 tgtggcacaa ttctctcctt tagactcagt acatcattgc cgggggtaag taagggctcc
     1261 ctctccgttc atcggttttg gagcagataa gttacactcg gattctcagc ccaactcacc
     1321 cgtctgtcaa taggagaaag agagtgatcg gacaccatcc ttgcggtatt cgatgcggct
     1381 gccttcccgc gttgagcatc gcgcggacga gttacggagt cgccggatga cgagctggag
     1441 tcccctttcc cacggacagg aagcgctctg gtttttgtgg aagctcgtcc ccgctacgcc
     1501 gacgtacaac atcgctcttc cggttggtgt gcgcggcgcg ctcgacgtcc gcgcgtttcg
     1561 tgccgcgctg cagtcgctgt cggatcgcca cgctgcgcta cgtctcgagt tccgcgaaga
     1621 aaacggcgct ccgatgcagc gcccgatcga ccggcacgag gtgctgctcg accgcatcga
     1681 tgcttcggat tgggacgatg cgatggtagg cgcggccgtc cgcgagcgtg cacggcagcc
     1741 gttcgatctc gaggccagcg cggcgatgcg cgccaccctc ttccgccgct ccgacgatca
     1801 ccacgtcctc ctcatcgtcg tccatcacat gaccagcgat ctctggtcgc tcatcgtggc
     1861 gatggacgag ctgcgagagc tttacgcggc ggagaaagag gaccgagaac cggcgctcgc
     1921 tcctcgatcc acgacctaca tcgatgcgat ccgtcagcag cgcgagacgc tcgagggcga
     1981 aacgggcgag cgcctctggc agttctggcg cgacgagctc tccggcgacc tgccggcgct
     2041 cgatctgcca gccgcgcgtt tgcgtccgtc gatgcagtcg ttccgcggcg gaacgatcac
     2101 gcggcggatg agcgtctccc tcacaccgcg gctcaagcag ctcgccggcc gcgaacgggt
     2161 gactctctat atgttgctgc tggccgcgta cgaagtactg ctgcaccgat acacgggaca
     2221 ggaggccttc atcgtcggct ccccaacctc ggggcgcgag cgtcccgaac tgcaggatgt
     2281 ggtcggcgac ttcgtgaaca tggtgccgct gcgcgcggac ctctccgcat caccgacgtt
     2341 ccgcgaggtc ctcggccgcg ttcgcgcacg cgtgctcggc acgatgcaac atcaggacta
     2401 ccccttctcg ctgctcatcg accgcctcca tccggcgcgc gatctgagcc gctcaccgct
     2461 gttccagacc acgttcgtgc tgcagaagtt tcatcgcttc caggagctgt cgcgcgtgat
     2521 gctgccgggt gacggcgagg cggcgattcc gttcgccgat ctgctgctcg agccgatgcc
     2581 gctcgcgcag caggacggcc agttcgatct caacgtggag atgaaagagg acgatcgcgg
     2641 gcaactcgtg ggcgcgtgga agtacgcggc cgatctgttc aacgcgccgg cgatcgagcg
     2701 gatggccgcg cacttcgaga cgctgctcga gtcgatcgtg gccgaaccga attgcgcaat
     2761 cggggcgctg ccgctgctcg acgccgtcga aagccacgat gcgatcgcgc gtggtgcggg
     2821 agacgtgacg gaactgcccg acaagacggt ctgcgaactc ttcgaagcgc agtcgctgcg
     2881 gcgccccgat gcggtcgccg tcacctgcgg cgacgacatc gtgacgtacg gcgaactgca
     2941 cgaccgggtc gccagtctgg
//
LOCUS       DATLVW010000352         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_223090, whole genome shotgun sequence.
ACCESSION   DATLVW010000352 DATLVW010000000
VERSION     DATLVW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223090"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VNN25_10670"
     CDS             <1..352
                     /locus_tag="VNN25_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92033.1"
                     /translation="LLLADIIQNDIPQTGTAPIFSFTTRPYIDIIIPARQLARERGGV
                     TDATLMFYIFDAGGNAVEYREKKISIPAVPSSDIAVRQKLVMPPGSYVAKALLRIDKS
                     IGFAKVAFAIPAEK"
     gene            complement(363..1547)
                     /locus_tag="VNN25_10675"
     CDS             complement(363..1547)
                     /locus_tag="VNN25_10675"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HXH92034.1"
                     /translation="MSLMSTLQTELDQMAAAGTLKRELLMKSPQGPIVDVEGFGEVIM
                     LTSNNYLGLADHPAIGEAAAKAEHDYGYGLASVRFICGTQTIHRQLEQRIAAFFGTED
                     TILYGSCWNANEGLFQTVAQEQDAIISDELNHASIIDGVRLAKAKKERYKNRDMADLR
                     RALEATRDSRNKLIMTDGVFSMEGSLAPLPEMAALAAEYDAFLVVDDSHGTGVLGKTG
                     RGTAEELGVLDKIHAHTSTLGKALGGSHGGFTTGPKTLVDYLRQKSRPYLFSNTLPPA
                     VVMGSLAALDLLENDPSFVTRLRENTTYFRQQLTAKGVAVREGIHPIVPIIIGDTAKA
                     IAISAELLRRGVYVSGFGFPVVPQGAARLRCQISAAHTREHLDRAIEAIVEVGRKFEV
                     VQ"
     gene            1602..1796
                     /locus_tag="VNN25_10680"
     CDS             1602..1796
                     /locus_tag="VNN25_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92035.1"
                     /translation="MASGVAWFAATANWRRDIQDLILSNLDIFIQNLSAEKRDNTADE
                     RDFRWENASTCRVVAPIRRV"
     gene            complement(2101..2469)
                     /locus_tag="VNN25_10685"
     CDS             complement(2101..2469)
                     /locus_tag="VNN25_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH92036.1"
                     /translation="MPAIALHHVQVAIPRGEEDRARAFYGGVLGLIEIPKPANQRERG
                     GLWYRVGALELHIGVEDDFRPAKKAHPALLVDDLAAVVALAEAAGHPSKPDTLVIEGY
                     DRVFVSDPFGNRIELLQRLG"
     gene            complement(2475..3110)
                     /locus_tag="VNN25_10690"
     CDS             complement(2475..3110)
                     /locus_tag="VNN25_10690"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HXH92037.1"
                     /translation="MDLHNSPATSAQPVYSPAPVPVEQFIDSIRELSMGVITKQKIYD
                     FLATYQIRDEDLERYKMWLPERHTRNKVFRNDMIEAMVICWPVGTITPLHTHNGQLGW
                     MTMIEGRLIVENYKKVDCNRPENQQVVGMDCLAGATRIEMQALGTELCVPGGPVNTVD
                     KTQTIHRIKNLAEWNERAVSLHVYSRPIDSCVVFDIEGGTCFRRDLKYDNE"
     gene            3303..>3395
                     /locus_tag="VNN25_10695"
     CDS             3303..>3395
                     /locus_tag="VNN25_10695"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HXH92038.1"
                     /translation="MKNLAVIGAQWGDEGKGKIVDLLCESFDVVA"
BASE COUNT          618 a         1015 c         1045 g          717 t
ORIGIN      
        1 gcttctcctg gctgacatca tccagaacga cattccgcag acgggaacgg ctccgatatt
       61 ctcattcacg acgcgtccgt acatcgacat catcattccg gcgcggcaac tggcccggga
      121 gcgcggcggg gtcaccgacg ccacgctcat gttctacatt ttcgacgcag ggggaaacgc
      181 ggtcgaatac cgcgagaaga agatctcgat cccagcggtg ccttcttccg acatagccgt
      241 ccgccagaaa ctggtcatgc cccccggcag ctacgtagca aaggcgctgc tgcgcatcga
      301 caagtcgatc ggcttcgcga aggtggcgtt tgcgattccg gcggagaagt gacgcacagc
      361 agctactgaa cgacctcgaa tttcctgccc acctcaacga tcgcctcgat ggctcggtcc
      421 aggtgctccc gtgtgtgtgc ggcgctgatc tggcaacgca ggcgtgccgc gccctggggc
      481 acgaccggga agccgaagcc ggagacgtag acgccgcgcc tcaacagctc ggcggagatg
      541 gcgatcgctt tcgcggtgtc gccgatgatg atcggaacga tgggatggat tccttcgcgc
      601 acggccacgc ccttcgccgt gagttgctgg cgaaagtacg tcgtgttttc gcgcaggcgc
      661 gtcacgaagc tcggatcgtt ttcgagtaaa tcgagcgcgg ccagcgatcc catgaccacc
      721 gccggcggga gcgtgttgga gaacaggtac ggccgcgatt tctggcgaag gtaatcgacc
      781 agcgtcttcg gtccggtcgt gaatccgccg tgcgatccgc ccagcgcttt gccgagcgtc
      841 gatgtgtgcg cgtggatctt atcgagcacg cccagctcct cggccgttcc gcggcccgtc
      901 ttgccgagga cgccggtacc gtgcgagtca tcgacgacga gaaaggcgtc gtactccgcg
      961 gccagcgcgg ccatctccgg cagcggcgcc agcgaccctt ccatcgagaa gacgccgtcg
     1021 gtcatgatca gtttgttgcg cgaatcgcgc gtcgcttcga gcgcgcggcg caggtcggcc
     1081 atgtcgcgat tcttgtagcg ctccttcttc gccttcgcca ggcgcacgcc gtcgatgatc
     1141 gaggcgtgat tgagctcgtc ggagatgatc gcgtcctgct cctgcgccac ggtctgaaat
     1201 agtccttcgt tcgcgttcca gcaggagccg tagaggatcg tgtcttccgt cccgaagaac
     1261 gcggcgatgc gctgttcgag ctggcggtga atcgtctgcg tgccgcagat gaagcgcacc
     1321 gaggccaggc cgtagccgta gtcgtgctcc gctttcgcgg ccgcttcgcc gatcgccgga
     1381 tggtcggcca ggccgaggta gttgttggag gtgagcatga tgacttcgcc gaatccttcg
     1441 acgtcgacga tcgggccctg cggcgatttc atcagcagct cgcgcttgag cgtgcctgcg
     1501 gcagccatct gatcgagctc ggtttgcagc gtggacatca gcgacattgc gtcacctcgg
     1561 cgggttggaa ttcgggcggc ggattgtatc agcgggggcg ggtggcttca ggtgtcgcct
     1621 ggtttgccgc gacggctaac tggcgacgag atatccaaga tttgattttg tcgaatctcg
     1681 atatcttcat ccagaatctg tcggcggaaa aacgcgacaa cacggcggat gaacgcgatt
     1741 ttcggtggga aaacgcgtca acctgccgtg ttgtcgcgcc catccgccgt gtttaatgcg
     1801 tttttcggac gaaaatcgcg tttttcgccc gaaaatcacg tttttcggat gaaaatcggg
     1861 ttcatccgcc gtgttaacgc gtttttcgga cgaaaatcgt gtttttcgcc tgaaaatcac
     1921 gtttttcgga tgaaaatcgg gttcatccgc cgtggacgcg tttttcggac gaaaatcgtg
     1981 ttttttgcct gaaaatcacg tttttcgcat gaaaatcgcg tttttcgccc gaaaaacacg
     2041 atttatctat ggaaacatca ggccaatccg tcctaaatct gtgacaaaca attctgatcg
     2101 ctagcccaat ctctgcagca gctcgatccg attgccgaac ggatcgctga cgaacacgcg
     2161 gtcataacct tcgatcacga gagtatccgg cttcgatgga tggcctgctg cctcggccag
     2221 cgcgacaacg gccgcgaggt catcgacgag tagtgcagga tgcgctttct tcgccgggcg
     2281 gaaatcgtcc tcgacgccga tgtgcagttc gagcgcgccg acgcgatacc aaaggcctcc
     2341 gcgctcccgc tggtttgccg gcttcggaat ctcgatcagg ccgagcacgc caccatagaa
     2401 cgcgcgggct cgatcctctt cgccccgcgg aatggccacc tgaacgtggt gaagagcgat
     2461 ggcgggcacg cggtctactc gttgtcgtac ttcagatcgc gtcggaaaca ggtaccgcct
     2521 tcgatgtcga agacgacgca ggaatcgatc ggacgcgagt agacgtggag gctgacggcg
     2581 cgctcgttcc actccgcgag attcttgatg cggtgaatcg tctgcgtttt gtcgacggtg
     2641 ttcaccggac cgcccgggac gcagagctcc gtgccgaggg cttgcatctc gatgcgggtg
     2701 gccccggcca gacagtccat gcccaccacc tgctgattct cggggcggtt acagtcgact
     2761 ttcttgtagt tctcgacgat gagcctccct tcgatcatcg tcatccagcc gagctggccg
     2821 ttgtgcgtgt gcaggggagt gatcgtgccg accggccagc agatcaccat cgcctcgatc
     2881 atgtcgttgc gaaagacttt gttccgtgtg tggcgctcgg gcagccacat cttgtagcgc
     2941 tcgaggtcct cgtcgcggat ctggtaggtg gccagaaagt cgtagatctt ctgcttggtg
     3001 atgacgccca tcgaaagctc gcggatgctg tcgatgaact gttcgaccgg aaccggcgcc
     3061 ggcgagtaaa ccggctgagc ggaagtggcg gggctgttgt gcaggtccat gcgggttcct
     3121 cggggtgcaa ctatatacgc gggtgtcggt tgtcgggagt cgggggtcgg agacagactc
     3181 taccgcctgt gctgcagaac agctgtccct gcacaaggct tccaaaatct gactggcagc
     3241 gctctctaag acccccgact cccgaccccc gactccccct cccatgctag tctccccgcc
     3301 gcatgaaaaa ccttgcagtc attggcgccc agtggggcga cgaggggaag ggaaagatcg
     3361 tcgatcttct ctgcgaatcc ttcgacgtcg tggcc
//
LOCUS       DATLVW010000353         4701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_348887, whole genome shotgun sequence.
ACCESSION   DATLVW010000353 DATLVW010000000
VERSION     DATLVW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348887"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VNN25_10700"
     CDS             <1..562
                     /locus_tag="VNN25_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92039.1"
                     /translation="TSYGLGIRTKDASATGPSLMRNMDIDRMMNKTGSVYEALDLLGD
                     ALRPIRARKDVVLFSNGIIGPDEDVRGGIVLNRSRYYDPMIESLNSADVTLYPVSLVS
                     DFDAPAFVHQNLATMASDTNGHYYRLHTSFTTPLQLIEKRTAGYYLISYYTKKRPGEH
                     GFQKVDVKLRNPEFKVSARAGYAYGD"
     gene            573..998
                     /locus_tag="VNN25_10705"
     CDS             573..998
                     /locus_tag="VNN25_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92040.1"
                     /translation="MRRSLDLIPRLAAALPLCMAFLLLAPPANARNRDVSGHYERNAE
                     NDQASLDITMLPDDRVKVDGVALWNIKNEKYGPNLGELTFEAPLKDDTVTWHDADGYS
                     VTIHFSRKALDVTEHEVDGHFGHNVTFVGHYVRTAHQTD"
     gene            1038..1307
                     /locus_tag="VNN25_10710"
     CDS             1038..1307
                     /locus_tag="VNN25_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92041.1"
                     /translation="MQKETQISAVVSQSTRDLLERHVRATGVKKGHLVEQALRHHLQA
                     LQEIPAEFIIHPKIVVTRRSGAAILKKMRSGKATKALRDLMRNGD"
     gene            1297..1815
                     /locus_tag="VNN25_10715"
     CDS             1297..1815
                     /locus_tag="VNN25_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92042.1"
                     /translation="MEIRHLREEDDRSHFQSGTPELDRFFRQFAGQNQFKHHIGVTYV
                     AIDNGRILGFVTIAAGHIEMEDLPTSERKRLPRYPLPVVRLARLAVDASARGQGVGAK
                     LLRFVVILAVRLANDYGCIGVVVDAKPDAIDFYSRYGFIAADALEGQSDARPQPVMMY
                     LSLHEIEESIGE"
     gene            1873..2754
                     /locus_tag="VNN25_10720"
     CDS             1873..2754
                     /locus_tag="VNN25_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92043.1"
                     /translation="MKKIVAALLVTSLALASCKKKEEAAAPQPAAPTPAATTSSATPA
                     PAPKAATPIPSVPLAADAPIPGHGLALWLTGDDAMKSATAGKVASWTNPLVPNVSASP
                     TEGTAPPSVVANAINGHAAVRFDGSTNMLRTTIDISPASWPDVTVITVFNSVTDVKNP
                     LRSVYGDDNGDYDRAAGLDDRSGDEKNYVIFAGDRGVAGYFALKANQVYVTTDQYTAT
                     EFNGWVDGKPAITKLATHWGEALPNMYIGGTGTSYEQEWQGDIAEMIVYARVLTDAER
                     TKVEDYLAKKYGVTLTR"
     gene            2788..3102
                     /locus_tag="VNN25_10725"
     CDS             2788..3102
                     /locus_tag="VNN25_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH92044.1"
                     /translation="MRAVMSAHIQLDPAFVTEFCQRHHVRRLSLFGSVLTDRFRPESD
                     LDVLIEFEREDTPGLFALARMQFEIEAVVGRPVDLRTPEDLSRHFREDVIRSAEVQYS
                     AV"
     gene            3122..3439
                     /locus_tag="VNN25_10730"
     CDS             3122..3439
                     /locus_tag="VNN25_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXH92045.1"
                     /translation="MLEAARKIARFTSKHRSAEELLADEADFWSVVHMLEVIGEAAAH
                     VSTVTRAALPLPWLESVAARNRLAHGYFNISALVIWRTATEDIPLLAHALETFFDREA
                     RGE"
     gene            3444..>4701
                     /gene="hydA"
                     /locus_tag="VNN25_10735"
     CDS             3444..>4701
                     /gene="hydA"
                     /locus_tag="VNN25_10735"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HXH92046.1"
                     /translation="MTTTLIKNGRIVTAVDDYNADILIDDGTIAMIGRSIDVAADKVI
                     DAKGRLVIPGGIDPHTHMELPFGGTSASDTFETGTIAAAHGGTTTIIDFAVQYKGQSL
                     KQAVDAWHAKADGRTSIDYGFHLICTDLPDERLPEMKSMIDEGVSSFKLFMAYPGVFL
                     VDDATIFKAMGVAGENGGLICMHAENGVVIDAIVKKALAEGKTAPKYHALTRPTKAEA
                     EGVHRAIALAEMAGSSVYIVHLSCDDSLQEVTRARDMGINAYAETCPQYLFLDYTVYE
                     QPGFEGAKYVMTPPIRDKWNQEQLWRGLKFNDLQVVSTDHCPFCMKEQKELGKNDFSK
                     IPNGGPGVEDRMSLVYNGGVAEGRISVNRFVEITSTAAAKIFGLFPRKGTIAVGSDAD
                     IVIFDPNEERTISAKTHHMNVDYSCYE"
BASE COUNT         1008 a         1414 c         1415 g          864 t
ORIGIN      
        1 gacctcctac ggcctcggca tccgcaccaa agatgcatcg gcaactggcc cctcgctgat
       61 gcgcaacatg gacatcgatc gcatgatgaa caagaccggc tcggtctacg aggcgctcga
      121 ccttctcggt gatgcgctgc ggccgattcg tgcgcggaag gacgtcgtgc tcttctcgaa
      181 cggcatcatc ggtccggacg aggacgtgcg tggcggcatc gtgctcaatc gaagccgcta
      241 ctacgatccg atgatcgaat cgctcaacag cgccgacgtc acgctctatc ccgtctcgct
      301 cgtcagcgac ttcgacgccc cggcgttcgt acaccagaat ctggcgacga tggccagcga
      361 cacgaacggc cactattatc gcctgcacac gagctttacg acgccgctgc aactgatcga
      421 gaagcgcacc gcgggctact acctcatcag ctattacacg aagaagcggc cgggcgagca
      481 tggcttccag aaggtcgacg tcaagcttcg caatccggag ttcaaggtca gcgcgcgggc
      541 gggatatgcg tacggcgatt aaccatcgcg tcgtgcgtcg ttcgctcgat ctgattccgc
      601 ggctggcggc tgcgttaccg ctctgcatgg cgttcctgct actcgctccg cctgccaacg
      661 cccggaaccg cgacgtctcc ggccactacg agcgcaacgc cgagaatgac caggcctcgc
      721 tcgacatcac gatgcttccc gacgaccgcg tgaaggtcga tggcgtcgca ctctggaaca
      781 tcaagaacga aaagtacggc cccaacctcg gcgagctcac tttcgaagcc ccgctgaaag
      841 acgacaccgt cacctggcac gacgcggacg gctacagcgt gacaatccac ttctcccgca
      901 aagccctcga cgtcacagaa cacgaagtcg acggccactt tggccacaac gtgacgttcg
      961 tggggcacta cgtgaggacc gcgcatcaaa cagattgacg gaattcgtac agttatgtag
     1021 attcctgatg aatagccatg caaaaggaaa cacagatttc ggccgtcgtg tctcaatcaa
     1081 caagggacct gcttgaaagg catgttcgcg ctacgggagt gaagaaaggg cacctcgtcg
     1141 agcaagcgct tcggcatcat cttcaagcgc tgcaagagat accggcggag ttcatcatcc
     1201 atcccaagat tgtcgtgacg cggcgatcgg gtgcggcgat cctgaagaag atgagatccg
     1261 gcaaggctac aaaagcgctg cgtgacttga tgcgcaatgg agattagaca tctccgcgag
     1321 gaagacgacc gctcacactt ccagtcgggc actcccgagc tcgaccgctt cttccggcag
     1381 ttcgcaggtc agaatcaatt caagcatcac atcggcgtga catacgttgc cattgacaat
     1441 ggccggatcc tcggcttcgt gacgatcgct gctggccaca tcgagatgga agacttgcca
     1501 acttctgagc ggaagaggtt gccgcgatac cctcttccag tcgtccggct ggcccgcctt
     1561 gccgtcgatg catcggcccg aggccaaggg gtcggagcga aactcctgcg gtttgtagtc
     1621 atcctcgccg tgcgcttggc gaacgactat ggctgcatag gggtggttgt cgatgccaaa
     1681 ccggacgcga ttgacttcta ctccaggtat ggattcattg ccgctgatgc tttggaggga
     1741 cagtccgacg cgcgaccgca gccggtgatg atgtatctat ccctccatga gatcgaagag
     1801 agcatcggcg aatgatcgca ggtatggcga actgcgcttg ttgagagcca cattttggta
     1861 cgattcttgg ccatgaaaaa aatcgtcgca gcattgctcg tcacgagcct cgctctcgcc
     1921 agttgcaaga agaaggagga ggcggccgct ccccagcctg ccgcgcccac gccggctgcg
     1981 acgacatcga gtgctacgcc cgcgccggcg ccgaaggcag ccacgccgat accaagcgtc
     2041 ccgctcgctg cggatgcgcc gatacccggc cacggcctcg cactctggct gaccggcgac
     2101 gacgccatga agtccgcgac tgcggggaag gtggcctcct ggacgaaccc gctcgtaccg
     2161 aacgtctccg catcacccac cgaagggaca gcgccaccat ccgtcgtggc gaatgccatc
     2221 aacggccacg ccgcggtgcg ctttgacggc tcaacaaaca tgctgcgcac gaccatcgac
     2281 atcagtccgg cgtcgtggcc tgacgttacc gtgatcacgg tgttcaacag cgtcaccgac
     2341 gtgaagaacc ctctgcgcag tgtgtatggc gacgacaacg gcgactacga tcgcgctgcg
     2401 ggactcgacg atcgcagcgg tgacgaaaag aactatgtga ttttcgccgg cgatcgcggc
     2461 gtggccggct acttcgcgct gaaagcaaac caggtctacg tcaccaccga tcaatacacc
     2521 gccaccgagt tcaacggctg ggtcgacggg aagccggcaa tcactaagct cgccacacac
     2581 tggggcgagg cgctaccaaa catgtacatc ggtggaaccg ggacgagcta cgaacaagaa
     2641 tggcagggcg acatcgccga gatgatcgtc tacgcccgcg tgctcaccga cgcggagcgg
     2701 acgaaggtcg aggattacct cgcgaagaag tacggggtga cgttaacgcg ctaagtcaca
     2761 cattccgttg cttgatcgcg cggtagaatg cgagccgtca tgtctgcgca cattcagctc
     2821 gatccggcgt tcgtcaccga gttttgccaa cggcaccacg ttcggcgtct ttcgctgttc
     2881 ggctcggtgc tgacggacag gttccggccc gagagcgacc tcgacgtcct catcgaattt
     2941 gagcgtgagg atacgcccgg gctttttgct ctggcgcgta tgcagttcga gatcgaagcg
     3001 gttgttggca gaccagtgga cctccgcacg cctgaagatc taagccgcca ttttcgtgag
     3061 gatgtaattc ggtcggctga ggtgcagtat tccgccgtct gatcgggcgc ggttggagca
     3121 catgctcgaa gcggcgcgaa aaattgcgcg cttcacgagt aaacacagat ccgccgaaga
     3181 actacttgct gatgaagccg acttctggag tgtcgttcac atgctcgaag tgatcggaga
     3241 agctgcggct catgtcagca cagttacgcg tgcagcgctt ccattgccct ggttggagag
     3301 cgtggccgcc cggaaccgcc tggcacatgg ctacttcaat attagcgcgc tcgtaatctg
     3361 gcgaacggcg accgaagaca tcccgctact tgcgcatgca ctcgagacat tctttgatcg
     3421 agaagctaga ggagaatgaa cccatgacca caaccctcat caaaaatggc cgcatcgtca
     3481 ccgcagtcga cgattacaac gctgacatcc tcatcgacga cgggaccatc gccatgatcg
     3541 ggcggtcgat cgacgtcgcg gcggacaagg tgatcgatgc caaaggccgg ctggtcattc
     3601 ctggcgggat cgatcctcac acgcatatgg agctgccgtt cggcgggacc tcggcgtccg
     3661 atacgttcga gacggggacg attgcggcgg cgcatggcgg gacgacgacg atcatcgact
     3721 ttgcggtgca gtacaagggg cagtcgctga agcaggcggt ggatgcgtgg cacgccaagg
     3781 ctgatgggcg gacgtcgatc gactatgggt ttcatctcat atgcaccgat ctgcccgacg
     3841 agcggctgcc ggagatgaag tcgatgatcg atgagggtgt gtcgagcttc aagctcttca
     3901 tggcctatcc gggagtcttc ctcgtcgatg acgccacgat cttcaaggcc atgggcgtgg
     3961 cgggggagaa cggtggcctg atctgcatgc acgccgagaa cggcgtcgtc atcgatgcca
     4021 tcgtcaagaa agcgctggcc gaggggaaga cggcgccgaa gtatcacgcg ctgacgcggc
     4081 cgacgaaggc ggaggccgag ggcgtgcatc gggccatcgc gctggcggag atggccggtt
     4141 cttcggtcta catcgttcac ctcagttgcg atgactcgct gcaggaagtg actcgggcgc
     4201 gggacatggg catcaacgcc tacgccgaga cctgcccgca gtatctcttc ctcgattaca
     4261 ccgtttacga gcagcccggt ttcgagggcg ccaagtacgt aatgacgccg cccattcgcg
     4321 acaagtggaa ccaggagcag ttgtggcgcg ggttgaagtt caacgacctg caggtggtct
     4381 cgaccgatca ctgtccgttc tgcatgaagg agcagaagga gctgggcaag aacgacttct
     4441 cgaagatccc gaacggcgga ccgggagtcg aagacaggat gtcgctcgtc tacaacggtg
     4501 gcgtagccga gggccgcatc tcagtgaatc ggttcgtcga gatcacttcg accgcggcgg
     4561 cgaagatctt cgggctcttc ccacgaaaag gaacgatcgc cgtcggcagc gacgccgata
     4621 tcgtcatctt cgatccgaac gaggagcgca cgatcagcgc gaagacgcat cacatgaacg
     4681 tcgactactc ctgctacgaa g
//
LOCUS       DATLVW010000354        13138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_385116, whole genome shotgun sequence.
ACCESSION   DATLVW010000354 DATLVW010000000
VERSION     DATLVW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385116"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5533
                     /locus_tag="VNN25_10740"
     CDS             <1..5533
                     /locus_tag="VNN25_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92047.1"
                     /translation="GASYQWRNFGTPLVGANAQTFNATTSGSYSVTVSDATGCSATSS
                     NVSVTANTTVNVTISAGGPTTFCSGGNVTLTANPSGGSGTFSSYQWLLNGSPIGGANG
                     TTYSATATGNYSVTVADSAGCTGTSAPQSVTVNTPPSAGISAPASVCPNSTAAASITS
                     PVGGATYTWSVTNGTLQTGQGTSSITFSVGTSSPVSISVTATANSCSSTGSASISVGV
                     FTPTITPSGSASFCAGGSVTLTSSSAASYQWSLNGTPIGGATSQTYAAPVAGSYTVTA
                     TSASGCSGTSAAVTVTQNATPAPTVTAGGPTTFCSGGSVTLSTGVASSYQWLSNGSPI
                     SGANGQSYSATLSGSYSVTVTNASGCSGTSSAVSVTVNPNPVVNITGPSSICGSGNVT
                     LDAGAGFASYNWSNGALTQTITVAPTTTTTYSVTVTTSAGCSGSASKTVTVSSGASTT
                     TVTAPSSVAASSAGNTASVPPAATGATYNWSITGGTITNGNGTTSIIWTAGASGNAVI
                     SVVVTSGSCTASGSATVAITDAADLALTAIAGPNPVNAGGTVTITLNVTNNGPNTALN
                     ARITNNLFGVSAPVASGNGWSCTTSNLTVVCSAASLPPGPAAPLTITATASAANSAGD
                     DAYVLADTAESNGTNNSASTSVSIAAQPSCNNSAPSLVSPAAGAANVANPVTFTWSST
                     ANALSYDLWIASGGAAPAIAATTQSTTALLSLPSGPSTWYVAAHFAGDCPTLFSAPRD
                     FTVAAATNCTHDAPQLIAPSPNAVVNSPVTFNWTAVTQAVGYRVWVAIDGGAAQDVGT
                     TNGATSLTVALSGSSIAWHVDANFPGCNPTSSATSTFSIAAVDACGNHGAPSLVAPAA
                     GATLASSIVDLQWSAVANANGYRVWAGVNGAVPAPIGTTATATTLHATFSSGAVVWFV
                     EALFNGCASSPSAQATFTIPAAQNCNNAAPTPLAPSNDATLTNSEVDFSWSGVPNAIG
                     YELWLALNNGTPSPAGTTTATTLRRTVGAGAVEWFVRAQFNGCDPVESAHAHFTFAPP
                     ADCALVRPILTAPVDGAPVVFSPVDLQWNNVPGATQYKVWLGNGDTPPVTIGTTTATH
                     LDNQTIPAGSVKWFVEALFDHCPALQSTVSTFTAVPPPPPCSTPSVPLPRAESTASTN
                     VEYVIRWTPAGPQATTKYDLQESTDAAFSAPLSITTDASEWAFKHINSTDAPIPYYYR
                     VRAVGTCNGARSLFSQPLTVSVLPSSVTDPSSANGSTPADNPQTTHYEIHIGGTSGNS
                     VHKGAIIAAAGDSFTATTNQPWLTVSPSSGTVGVDGTTLNVTAKTDGLPVGTSSGAVS
                     IAFASASGAGNRVALGTTPPANTTVSVNLVQPVSPIAKSGPPPDALIIPAVAHAAGIN
                     SKFQSDIRVTNSSAQPMKYQVTFVPDGDAGITQGKQTTVDIDPGRTIALDDVLQSWFG
                     DSAATGTLEVRPLTTAPTSSTATALPAGLANILTFASSRTYNTTANGTFGQYIPAIPF
                     AAFVGRGSDPTKSTILSLQQIAQSSAFRTNLGIVEGSGDPASVLISVFGSNGQKLTEF
                     TQDLKGGQHIQLNSILATKNLQVTDGRIEVKVVSPAGKVTAYASVLDNLTNDPLLVSP
                     VAVNQTAAAKYVIPGVADLNNGVANWQTDVRIFNPSTNIVKATLTFYSQTGSAPQSKD
                     IQLAPGQVQTLDATLHNVFGISNDGGALHIATTAPTPLVATARTYNQTGNGTYGQFIA
                     AVTANDAAALGTRPLQILQIEESDRYRTNVGFAEVSGNAATVEVTAIPSDSKVAASTQ
                     IQLTPNQFIQFPQLLKSMGLSNTYNARVSVKVISGQGRVTSYASVIDMQTNDPTYVPA
                     Q"
     gene            5783..6358
                     /locus_tag="VNN25_10745"
     CDS             5783..6358
                     /locus_tag="VNN25_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92048.1"
                     /translation="MRDRATRGVKTPDSDDGKPTTRGKVDRDWFKRTIDLVTLAVVAA
                     GFYLGLDQAKKLTESIESSNRSNNLSTWSTITNQGMEIDKIFVDHPEFQPYFFEGVVI
                     RSKDPNFPRAKAIATAFLDYIDSATALLYQMKKTPTPVPFVEYDTWNHYFEVQFGTSP
                     LLCQTLFESKDIYGQEILKVGLPACRAKKYL"
     gene            complement(6669..7265)
                     /locus_tag="VNN25_10750"
     CDS             complement(6669..7265)
                     /locus_tag="VNN25_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92049.1"
                     /translation="MKANQSAFDAGQLAERLAQGYYSHLAAIAVATDPRALKLAPRIR
                     PGHTVPVGEVRRKDGTAHAFHFLHFLALAGSDAVIAGELERIWLTGALLRVGDVLAQH
                     QYFDRAPELELLRHLRNGAAHGNRFRIDHPASLARYPAHNRLALVRGDLKPVFEITPE
                     LQGQEVLFGYMGAGNVLDLLMSVGLYLVRMGNGDPLSP"
     gene            complement(7857..8054)
                     /locus_tag="VNN25_10755"
     CDS             complement(7857..8054)
                     /locus_tag="VNN25_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92050.1"
                     /translation="MPSLIEMGQAVLGLALLYMAFFLHEKEERQITERLGEWWIHVDD
                     AATAALAWHTKLFRVASQRLA"
     gene            complement(8396..8560)
                     /locus_tag="VNN25_10760"
     CDS             complement(8396..8560)
                     /locus_tag="VNN25_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92051.1"
                     /translation="MNNSTYIDGAEVHAGDRVAHGGVPATVVLVAERNEAVAEFALAE
                     WAEQGNILIR"
     gene            complement(9276..9707)
                     /locus_tag="VNN25_10765"
     CDS             complement(9276..9707)
                     /locus_tag="VNN25_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92052.1"
                     /translation="MGEDPNLFDVDAGHFARQFQDQVVPLAKQLIEFANDVVFTGQNL
                     LSASSVQGLQVYRLAQHVVKQPDGADLQPFVDTMSHIVKKTMNRRMKPAPAPQATPST
                     TTTSHTPPGAFRDSWRRTSSSTKAPARPMSRKMSRTVSNRH"
     gene            complement(10053..10514)
                     /locus_tag="VNN25_10770"
     CDS             complement(10053..10514)
                     /locus_tag="VNN25_10770"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXH92053.1"
                     /translation="MTEATAVPDAPARVGYAGKNRVLAMMVDYTLMFLLALLATKVLV
                     GGSLKGPIFVLVAFAYFFVPEWLWSATPAKAMFGLRVEQVQGGSCTLKGALSRTIFRV
                     LEANPVFLGPIPGGIAIMRSPLKQRLGDRFGGVLVVEKRTQGKSAPPVAAV"
     gene            complement(10792..11247)
                     /locus_tag="VNN25_10775"
     CDS             complement(10792..11247)
                     /locus_tag="VNN25_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92054.1"
                     /translation="MLLAVGIFAWLVLFAFALRGARWAYAIFVIFAFVWIPARTGFHL
                     HRPPCQMQFTADLALNSILKYKHISLWAAFFLMTWVQVRRSRYALPIAAIATMAVGIL
                     IELEEGATGTGMCRATDLLPDALGALTGALIATLWRKRQAKAVVMPPAG"
     gene            complement(11445..11861)
                     /locus_tag="VNN25_10780"
     CDS             complement(11445..11861)
                     /locus_tag="VNN25_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH92055.1"
                     /translation="MKRVIALGGMFAVGMVVGGLSTEFVHAQAPQYQTKQVLQTDLNN
                     LPGQEIIVFESTWQPGFRLPLHMHPNGHEITYVIEGEQTFEIEGVGTKVVKAGEAIYT
                     PPNTLHFGRNATDKISRTAVIRVKDKDKPIMVDATK"
     gene            complement(11900..12460)
                     /locus_tag="VNN25_10785"
     CDS             complement(11900..12460)
                     /locus_tag="VNN25_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92056.1"
                     /translation="MEPSADPYPKYLDWLRYLSAFLLLLYGTSKLLGRQFSVPPGLAL
                     RPVGSLSGRDLAWYYYSYSHIYATLLGLIQLAGAALLLFRKTALLGAAVVMPVITNIL
                     MINVFYYITWGATCTSTLIFAAMVAILWHDRHALLGVFWASQTSEPAELRRRHRMIAA
                     AVVLSVIVLMAVGLWLSAARTSALPR"
     gene            complement(12536..12835)
                     /locus_tag="VNN25_10790"
     CDS             complement(12536..12835)
                     /locus_tag="VNN25_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92057.1"
                     /translation="MSFINWSDSHEMLGLLSELVVDERNDSTDDPARAHFLRGVLEEL
                     GELTARIDETNEDDFIGTLRMILESTDGEFRGDPVLEHVQACIEELERIRDRVSA"
     gene            complement(12925..>13138)
                     /locus_tag="VNN25_10795"
     CDS             complement(12925..>13138)
                     /locus_tag="VNN25_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92058.1"
                     /translation="LKRYARVVVPSYIQFAVALLIFIAGRVAVVIVLHRPFLAGKLMG
                     VAIAVATVALCSLAFSQLHARPTTPN"
BASE COUNT         2714 a         4336 c         3683 g         2405 t
ORIGIN      
        1 cggcgcgtcg tatcagtggc gcaacttcgg cacaccgctc gtcggcgcca acgcgcagac
       61 attcaatgcc acgaccagcg gcagctactc ggtcaccgtt agcgatgcca ccggatgctc
      121 cgctacctcg tccaacgtct ccgtcaccgc aaacacgacg gtcaacgtca cgatctccgc
      181 gggcggcccg accaccttct gcagcggcgg caatgtgacc ctcaccgcga atcccagcgg
      241 cggcagcgga accttctcct cgtaccagtg gctgttgaat ggctcaccga tcggcggcgc
      301 gaatggaacc acctactccg ctacggccac cggaaactac agcgtaaccg ttgccgactc
      361 cgcgggctgc accggcacat ccgctccgca atcggtaacg gtcaacacgc cgccttccgc
      421 cggcatcagc gcacccgcat cggtctgccc caacagcacg gccgccgcca gcatcacaag
      481 tcctgtcggc ggcgcgacgt acacatggtc cgtcaccaac ggcacactgc aaacaggcca
      541 gggcacttcg tcgatcacct tctccgtcgg aacgtcctct ccggtatcga tcagcgtcac
      601 cgcaacggct aacagctgtt cgtcgaccgg cagcgccagc atcagcgtcg gcgtattcac
      661 gcccacgatc actccgtccg gcagcgcgtc gttctgcgca ggcggcagcg tcacgctcac
      721 ctcctcctcc gccgcgtcgt atcagtggtc gctcaacggc acgccgatcg gcggcgccac
      781 ctcgcagact tacgcggctc cggttgccgg cagctacacc gtaaccgcga ccagcgccag
      841 cggctgcagc ggaacatccg cggcggtcac ggtcacgcag aacgccacgc ccgctcccac
      901 ggtcacagcg ggtggcccga ccaccttctg cagcggcggt agcgtcacgc tctcgaccgg
      961 cgtcgcgtcg tcgtatcaat ggctcagcaa cggatcaccc atcagcggcg ccaacggtca
     1021 gtcgtactcc gcgacgctga gcggcagcta cagcgtcacc gtcacgaacg ccagcggttg
     1081 cagcggcacg tcgtccgccg tgagcgtcac ggtcaatccc aatccggtgg tgaacatcac
     1141 aggaccatcg tcgatctgcg gatcaggaaa cgtcaccctc gacgccggcg ctggcttcgc
     1201 gtcgtacaac tggtcgaacg gcgcgctcac ccagacgatt acggttgcgc ccaccacgac
     1261 gacgacctac agcgtcaccg tgaccacgtc cgcaggctgc agcggctcgg cctcgaagac
     1321 cgtcaccgtc tcgagcggcg cctcgacgac gaccgtcacc gcgccatcct cagtcgcagc
     1381 gtcctccgcg ggcaacaccg cctcggtgcc cccggcagcg acaggcgcga cgtacaactg
     1441 gtcgatcaca ggcggcacga tcaccaacgg caacggaacg acgtcgatta tctggaccgc
     1501 cggcgcgagc ggcaacgctg taatcagcgt cgtcgtcacc agcggctcct gcaccgcctc
     1561 ggggagcgcg accgtcgcca tcaccgacgc cgccgacctc gccctcaccg cgatcgccgg
     1621 cccgaatccg gtcaacgctg gcggcacggt gacgatcacg ctgaacgtca cgaacaatgg
     1681 gccgaacacc gccttgaatg cacggatcac gaacaatctc ttcggcgtaa gcgcaccggt
     1741 tgcgagcggc aacggctggt cttgcaccac ttcgaacctc accgtcgtct gctctgccgc
     1801 gtcgctgccg cccggccccg ccgcgccgct caccatcacg gccaccgcat ccgccgcgaa
     1861 cagcgccggc gatgatgcct acgtcctcgc cgataccgcg gaatcgaacg gcaccaacaa
     1921 tagcgccagc acctcggtct cgatcgcggc ccaaccttcc tgcaacaaca gcgcgccgtc
     1981 gctcgtctcg ccggccgcgg gcgcggccaa cgtcgccaac ccggttacgt tcacctggtc
     2041 gtccaccgcg aatgcgctct cgtacgatct ctggattgcc agcggcggtg ccgcccctgc
     2101 catcgccgcc acgacgcagt cgacgacggc attgctcagc ctccccagcg gtccgtccac
     2161 ctggtacgtc gcggcccact tcgccggcga ctgtccgacg ctcttctccg cgccgcgcga
     2221 cttcacggtc gccgcggcaa cgaattgcac tcatgacgca ccgcagctca tcgcgccgtc
     2281 gccaaatgcg gtcgtcaatt cgcctgtgac cttcaactgg acagcggtca cgcaggccgt
     2341 cggctatcgc gtgtgggtgg ccattgacgg aggcgcagcg caggatgtcg gcaccacgaa
     2401 cggcgcgacg tcgctgacag tcgcgctctc cggttccagc atcgcctggc acgtcgacgc
     2461 gaacttccca ggctgcaacc cgacttcgtc cgcaacctcc acgttcagca tcgccgccgt
     2521 cgatgcctgc ggcaatcacg gcgctccgtc gctcgtcgca ccggcagccg gagcaacgct
     2581 ggcgtcatcg atcgtcgatc tgcagtggag cgccgtggcc aacgccaacg gctaccgcgt
     2641 atgggccgga gtcaacggcg ccgtgcccgc accgatcgga accacggcga ccgccacgac
     2701 gctgcacgca actttcagca gcggagccgt cgtctggttt gtcgaagcgc tcttcaacgg
     2761 atgcgcgtcg tctccttcgg cgcaggcaac cttcacgatt cccgccgcgc agaactgcaa
     2821 caacgccgcg cccacaccgc tggcgccttc gaacgacgcc acgctcacca acagcgaagt
     2881 cgatttcagt tggtccggag tgccgaacgc catcggctac gaattgtggc tcgccctcaa
     2941 caacggaaca ccctctccgg ccggaacgac gaccgcgaca acgctgcgcc gcaccgtcgg
     3001 agccggggcc gtcgagtggt tcgtccgcgc gcaattcaac ggctgcgatc cagtggagtc
     3061 agcgcatgcg cacttcacct tcgcgccgcc ggccgattgc gcgctcgtcc gtcccatcct
     3121 caccgctccg gtcgacggcg ccccggtcgt cttctcgccg gtcgatctgc aatggaacaa
     3181 cgtgccgggc gccacacagt acaaagtctg gctcggcaac ggcgacacgc cccccgtcac
     3241 catcggtacg acgaccgcca cgcatctcga caaccagacg attcccgccg gcagcgtgaa
     3301 gtggttcgtc gaagcgctct tcgatcactg cccggcgctc caatccacag taagtacgtt
     3361 tacggcggtg ccgccaccgc cgccgtgttc aacgccatcg gttccactgc cacgtgcgga
     3421 gagcaccgcg tcgaccaacg tcgaatacgt gatccgatgg acgccggcag ggccgcaagc
     3481 gacgacgaaa tacgacctgc aggaatcgac cgacgccgcg ttcagcgcgc cgctctcgat
     3541 cacgaccgac gccagcgagt gggcgttcaa gcacatcaac agcacggacg cgcccatccc
     3601 ctactactac cgcgttcgcg cggtcggcac ctgcaacggc gcgcgcagcc tcttctcgca
     3661 gccgctgacc gtcagcgttc tgccgtcgag cgtgaccgat cccagctccg caaacggctc
     3721 aacgccggcc gacaacccgc agacgacgca ttacgagatc cacatcggcg gaacgtccgg
     3781 caactcagtg cacaagggtg cgatcatcgc agcggccggc gacagcttca ccgccacgac
     3841 gaatcaaccg tggctcactg tctcgccatc gagtggaacc gtcggcgtcg acggaacgac
     3901 gctgaatgtc actgcgaaga cggacggtct gcccgtcggt acgagcagcg gcgcggtcag
     3961 tatcgcgttc gcctccgctt cgggagccgg taatcgcgtt gcgctcggca ccacgccgcc
     4021 ggcgaacacc acggtctccg tgaacctcgt gcagccagtc tcgccgatcg ccaagagcgg
     4081 cccgccgccg gacgcgctca tcatccccgc cgtcgcgcac gccgccggca tcaactcgaa
     4141 gttccagtcc gacatccgcg tcacgaactc ctcagcgcag ccgatgaagt atcaggtcac
     4201 gttcgtaccg gacggcgacg caggcatcac gcagggcaaa cagaccacgg tcgacatcga
     4261 tcccggccgc accatcgcgc tcgacgacgt cctgcagtcg tggttcggcg acagcgccgc
     4321 caccggcacc ctcgaagtcc gtccgctcac cacggcgccc acctccagca cggccaccgc
     4381 actccctgcc ggtctggcca acatcctcac cttcgcctcc agccgcacgt acaacaccac
     4441 cgccaacggc acgttcggcc agtacatccc ggccattccg tttgcggcgt tcgtgggccg
     4501 aggaagcgat ccgacgaagt cgacgatcct gagcctccag cagatcgcgc agtcctccgc
     4561 cttccgcacc aacctcggca tcgtcgaagg ctccggcgat ccggcctcag tgctcatcag
     4621 cgtcttcgga tcgaacggtc agaaactcac cgagttcacg caggatctca aaggcggcca
     4681 gcacatccag ctcaactcca tcctggcgac gaagaacctg caagtcaccg acggccgcat
     4741 cgaggtcaag gtcgtttctc cggccggcaa ggtgacggcg tacgcctcgg tgctcgacaa
     4801 tctgaccaac gatccgctgc tcgtctcgcc ggtcgccgtc aaccagaccg ccgcagcgaa
     4861 atacgtgatc cccggcgtcg ccgatctgaa caacggcgtc gccaactggc aaaccgacgt
     4921 ccgcatcttc aatccatcga cgaacattgt gaaagcgacg ctgacgttct actcgcaaac
     4981 cggcagcgcg ccgcagtcga aagacatcca gctcgcgccc ggccaggtgc agacactcga
     5041 cgccacgctc cacaacgtct tcggtatctc caacgacggc ggcgccctgc acatcgccac
     5101 caccgcaccg accccgctag tagccaccgc ccgcacctac aaccagaccg gaaacggcac
     5161 atacggccag ttcatcgccg ccgtcaccgc caacgacgcc gccgccctcg gcacgagacc
     5221 actgcagatc ctgcagatcg aagaatcgga ccgctaccgc accaacgtcg gcttcgcaga
     5281 agtatccggc aacgccgcaa ccgtcgaagt caccgcaatc ccctccgact cgaaagtcgc
     5341 cgcctcaacc caaatccaac tcaccccgaa ccaattcatc caattcccgc agctcctcaa
     5401 atcgatgggc ctatcgaaca cctacaacgc ccgcgtcagc gtaaaagtca tcagcggcca
     5461 gggccgcgta acctcctacg cctcagtcat cgacatgcag accaacgacc caacatacgt
     5521 gccggcgcag tgaggagcgg attagtggtt agtagattcg tggatcagga ttcatttctg
     5581 atccacgctt tctgcctccc gtatcatcag gccaaggatc gcgttcgcat tcggctagcc
     5641 tcggttagcc cttcggttag cccgcatttc tcacactgca gagcgatgta cgctcccaac
     5701 cgcagcgagc gtgggctata ctttcttgag actcggctcg cgacgagcgt agtgaccacc
     5761 acttaactgg ggattacttc ggatgagaga cagagcaacc cgaggcgtca aaacaccgga
     5821 tagtgacgat ggcaagccaa caacaagagg caaggtggat cgcgattggt tcaagcgaac
     5881 catagatctc gtgacccttg ccgttgtagc tgcgggcttc tatctcggat tggatcaggc
     5941 gaagaaactc accgaaagca ttgaaagctc aaatcgaagc aacaacctct caacctggag
     6001 cactattact aatcagggaa tggagatcga caagatcttt gtggatcatc cagagtttca
     6061 accatatttc tttgagggcg tcgtcattcg gtcaaaggac ccgaacttcc caagggccaa
     6121 agcaatagcg acggcgtttc tcgactacat cgatagtgct acagcacttc tctatcaaat
     6181 gaagaaaacg ccaacgccag taccattcgt cgagtacgac acatggaacc attatttcga
     6241 agttcagttc ggtaccagcc cccttctgtg ccaaacactt ttcgagagca aggatatcta
     6301 tggtcaggag atcctgaaag tcggtttgcc ggcttgtagg gccaagaaat atttgtaagc
     6361 gagatcgacg tcacaccgtc ggtttcgaaa gcctctcgca cgcccggcgt aactcgcaca
     6421 ccttcttgga cttacgtcag gcacccatgg gcgatgcatt cgtgcattca gcctcgaatc
     6481 acacctaagt gattgcaaac tgcgtccttt ctccactcgg gcgacgatcg cccggatttg
     6541 ccgtgaatag tgtccgatgc tgtaagtctt ctagagatca acacttgcga gagaatgatc
     6601 cggaggcgcg aaaatgcgaa ggtgcgaccc tatcggcttc cttctaagtc ttctgtcggc
     6661 gccgcccatt atggactgag cggatcgcca ttccccattc ttactaggta aagacccact
     6721 gacatgagca agtccaatac gttcccagct cccatgtaac cgaataacac ttcttgtccc
     6781 tgtaactcgg gcgtaatttc aaagacgggt ttcaaatcac ctctaaccaa tgcgagacga
     6841 ttgtgcgccg gataccttgc gagactggcg gggtggtcga ttctgaatct gtttccatga
     6901 gctgcgccgt tgcgtaagtg ccgtaggagt tccagctctg gggcgcgatc aaaatattgg
     6961 tgctgtgcta gcacgtcgcc cacgcgcagc agagcaccgg taagccaaat tcgctccagt
     7021 tccccagcga tgacagcatc gctgcctgcg agcgcaagga agtgaagaaa gtgaaaggca
     7081 tgagctgtgc cgtcctttcg tcttacctcc ccgacgggaa cggtgtgccc aggccttatt
     7141 cgcggtgcta gctttaaggc cctcggatcg gtcgctactg caattgccgc aagatgagaa
     7201 tagtagcctt gcgccaaacg ctcagccaac tgacccgcat caaaggctga ttggtttgcc
     7261 ttcatacgga acaagccgcc caacagctca caggttcaac tgcccggccg ctcctataat
     7321 catagcgcga cacgcgacga agcgcacacc gacacccgct ccttaccgtc gggccgtcgg
     7381 gaactccgca agttacggat gatcgacctt ggagtcacgc cttcggtctt cgagagtgtc
     7441 ctcgaacgcg ggcataactt gcgcggcctc ttggacttac gtcatccatc cacagaagac
     7501 atcgtcgtgc ggtcatcctc gaattcacac ctaagtcatt gcaagcgcgt cgcttctctg
     7561 ctcggcgacg aatcgcaggg acttgccctg aattgtgttc gaggctgtaa gtcattcgga
     7621 gatcgaaact tgcgagcaaa tgatccggag cggcaaaaat acgaaggtgt gaccctgttg
     7681 gctccacttg ttaggacaag tacacaactt agaaagccca gtggaatgtc aggcagatct
     7741 tgcggcggaa caatgccttc accgaagagg aagacgaaag ccgctccact aagtaacatt
     7801 gctgtccaaa acgcgcttgc ggagattagc cgctcgccga acaagaagct tatgagctac
     7861 gcgagacgct gactggctac gcgaaacagt ttggtgtgcc atgccaacgc agcggtcgcg
     7921 gcatcgtcaa catgaatcca ccattcgcca agtcgctccg tgatctgacg ctcctccttt
     7981 tcatggagga agaaggccat gtaaagtagg gccaagccga gtacagcctg tcccatttcg
     8041 atgagcgagg gcacggcata gatgctagtg cgtggcgggg gtgatcgcaa gatatcgggg
     8101 tcagagcctt agggtcacac cttcggtctt cgagagtctc ttcgaacgcg ggcaaacttg
     8161 cgcagcttct tggccttacg tcagccgccc acaaaagacg tcgtcgtgcg gtcatccgaa
     8221 ttcacgcctt agttcgttgc aggcggcgtc gcttctccgc ttcgcgagga cgccacaacg
     8281 caggacgggc cacgtctgct gcaagcgaac gttacccgcc gggttcagcg cgagacagga
     8341 ggacggtgtc ttcttcgtcg tagaggtatt cgtacataaa caggtgtccg tcatcttagc
     8401 ggatgaggat gttgccttgc tcggcccact cagcgagcgc gaactctgcg accgcctcgt
     8461 tgcgttcagc caccaggact accgtcgccg ggactccacc gtgagcaaca cggtcgcctg
     8521 cgtggacctc ggcgccgtcg atgtaggttg agttgttcac ggtgctaacg tccctgctct
     8581 tcagcggtgc ggtgccccaa gtgccgaaga caggacgtga gaattttacg ctgaacgagg
     8641 cgcggagcgc cgggccatcg aacggcaccg cgtccgctgc aagggcgcgt tatccggcga
     8701 cgtgccactt cacgcccgcc gctgagattt cgaggacagt cgttctgctg acttccaacg
     8761 gcagccgtgc ttcgtcccat tcgattacta cgtaccgccg gccgcactta cgccagcttt
     8821 ggcagggtgc ttagttcggc ggctggtccc aaggaattga ggcgcgtcgc ttcagttcga
     8881 cgatagcagc accatacgtt tcaaattcgg cttcctgttc ggcatggatc tcgtcttcaa
     8941 tgatgaacat ttcaagccgc atagcggacc gcggttcagc gcttcgtgtc gcttgcagac
     9001 cgtggcggta agcctagcag atggcggcac gaagtccgct gcaaccgcca agttagcgcg
     9061 ccagtagcca gatggactct tcagtctgaa tgcgaacagt gccaacccga ggaggcgacg
     9121 atacgccgac ggcaaggcgg agtgtcgccg acccaggccg gagttccctt gagactccgc
     9181 cgcgagaaac gaagttcact tgctcgcctg aaggaacgag gggcatgctc gtggcagcat
     9241 gcccctcgta actcacggat ttttggtgac gtcttttaat gcctgttcga aacggtccgc
     9301 gacatcttcc ggctcattgg tcttgccggg gcttttgtcg aggacgaggt tcgacgccag
     9361 gaatccctga aagcgccagg cggggtgtga gacgtcgtcg tggtggaggg agtggcttgc
     9421 ggtgccggtg ccggcttcat gcggcggttc atcgtcttct tgacgatatg cgacatggtg
     9481 tcgacaaacg gctgcaagtc ggcgccgtcc ggctgcttga cgacgtgctg cgccaaccgg
     9541 taaacctgaa gcccctgtac ggaagaggcg gaaagcaggt tctggccggt gaacacgaca
     9601 tcgttggcga actcgatcaa ctgcttcgca agcgggacga cctgatcctg aaactgacgc
     9661 gcaaaatgcc cggcatcaac gtcgaacagg ttgggatcct cacccaagga acgctggcaa
     9721 ccaggttgat gacggtgtcg caatccctgg ggtcacacct tcggtcttcg agagtctcct
     9781 cgatcgcggg gataacttgc gcagcttctt ggacttacgt cggccacccg ccgaagacgt
     9841 cgtcgtgcgg tcttcctcga attcacgcct aagtcattgc aagcgcgtag cttctccgcg
     9901 cgacgaccaa tcgcagggac ttgctctgaa ttgtgtccga ggctgtaagt cattcggaga
     9961 ccgaaagttg cgagcaaatg atccggtgcg gcgaaaatgc gaaggtgtca ccctgctggt
    10021 cgactccggt tcgcgtccgg cgcgaaccga agttagactg cggcgaccgg cggcgcactc
    10081 ttgccctgcg ttcgtttttc cactaccagg accccgccaa agcgatcgcc cagtcgttgt
    10141 ttcagtggcg accgcatgat cgcaatccct ccgggaatcg ggccgagaaa cacgggattc
    10201 gcctcgagaa ctctgaagat tgttcgactc aatgcacctt tcagggtgca cgaaccgccc
    10261 tgcacttgct ccacgcggag cccaaacatt gcttttgcgg gtgtggcgga ccagagccat
    10321 tccggaacga agaagtacgc aaaggcgacg agaacaaaga tagggccctt tagcgaaccg
    10381 cccacgagca ccttcgttgc cagcagtgcg agcaagaaca tcagggtgta atcgaccatc
    10441 atcgccaaaa ctcgattctt gccagcgtaa ccgacgcgtg caggtgcatc cggaactgct
    10501 gtcgcttcgg tcatgagttt gtcgatccaa cgtccccagg tgagcggctt cggcccactt
    10561 cgagaacgtg agagtaagca tatcagcggg cgggccgaag tccgctccac ggcaaagtta
    10621 gccgccgcgc ttttgcgccg atcttcgcgg ttgatcagat ggacccgaag ggactctgct
    10681 ggctcattcg catttgacgg gtcaggtcta aagtctaaag tgccgaattg gactgatcgc
    10741 tgtaccgacg atcgtcctcg gcactttaga ctttagacct gacccccacg ttcaccccgc
    10801 cggaggcatt accactgctt tcgcctggcg ttttcgccat aaggtggcga tgagtgcgcc
    10861 ggtcagcgcg cccagggcgt cgggcaacaa atcagtggct cggcacatcc ctgtgccggt
    10921 tgctccttcc tcgagctcaa tgaggatgcc gaccgccatc gtcgcgatcg cagcgattgg
    10981 cagtgcatag cggctgcggc gaacctggac ccacgtcatc agaaagaacg cggcccagag
    11041 agaaatgtgc ttgtatttca ggatcgaatt gagagctagg tcggccgtaa actgcatttg
    11101 gcagggcggc cggtggaggt gaaaaccggt gcgcgccggg atccagacga acgcaaagat
    11161 cacaaagatc gcgtaggccc atcgagcgcc gcgcagggcg aaggcaaaga gaacaagcca
    11221 ggcgaaaatc ccgaccgcta gaagcatttt gtgcgattca tcgtatcagc gcggacgcct
    11281 tcttcatcgc actcaacctc gaatgcgtgg atatcggcct tcattggtgt cctggccgaa
    11341 ttgatgcgcc aacagcttcg acgcgaccgg ggtgctcggc gcaacgtcag ccacgccgga
    11401 ctgacagggc tgcccagatg cggacgttca atcatcaggt cgtcctactt cgtggcgtca
    11461 accatgatcg gcttgtcctt gtccttgact cggattactg cggtcctgga tatcttatcc
    11521 gtcgcattgc ggccgaagtg cagcgtgttg ggaggcgtat agattgcctc gcctgccttt
    11581 acgacctttg tcccgacgcc ttcaatttcg aacgtctgtt ctccctcgat gacgtatgtg
    11641 atctcatggc cgttcggatg catatgcagg ggcagtctga atcccggctg ccacgtcgat
    11701 tcaaagacga tgatttcctg acccggaagg ttgttcaaat ctgtctgtag aacctgcttc
    11761 gtttggtatt gcggagcctg agcatgaaca aactccgtcg acaggccacc caccaccatt
    11821 ccaacggcaa acattccacc cagcgcaatt acccgtttca ttgatttctc ccttcttccc
    11881 tcaggttaac cgctccaatt caccgcggca aggcgctggt acgggcggca gagagccata
    11941 agccgacggc catcaagaca ataaccgaca gaacaaccgc tgcggcgatc attcggtggc
    12001 gccttcgcag ttcggccggt tcgctggttt gactggccca gaagacgccg aggagtgcgt
    12061 gccggtcgtg ccagaggatg gccaccatgg cggcaaagat caacgtcgat gtgcacgttg
    12121 cgccccaggt gatgtagtaa aacacgttga tcatgaggat gttcgtgatc acgggcatca
    12181 ccacggccgc tcccaacaac gccgtcttgc ggaagagcag cagcgcggcg ccggccagtt
    12241 ggatcaatcc gaggagagtc gcgtagatgt gagagtagct gtagtaatac caggccagat
    12301 cgcggccgct cagggatcca accggcctga gagccagtcc cggtggtaca ctgaactgcc
    12361 tgccgaggag cttgctcgtt ccgtataaca ggagcaggaa ggcggagaga tagcggagcc
    12421 agtccagata cttcggatag gggtcggcgg atggttccat gtttcgctcc cttcgaggtt
    12481 gccgctttcg gtgacaggca tgaaggatag tcgacgaagg tgtgacgcta ttggctcagg
    12541 cactcactcg gtctcgaatg cgctcaagtt cctcgataca cgcttgaacg tgctccaaca
    12601 cagggtcgcc gcggaactca ccgtccgtcg actccaggat catccgaagc gttccgatga
    12661 aatcgtcttc attcgtctca tcgatgcgcg ccgtgagttc gcccagttct tccagcacgc
    12721 cgcgaagaaa gtgcgcccgc gccgggtcgt cggttgagtc gttccgctcg tcgacgacga
    12781 gctcggagag caacccaagc atctcgtggg aatcggacca gttgatgaag gacattcgag
    12841 gcctcccctc gccggctaac gctaacggat cacggctcac ccgtccggcg cgcgatgcgc
    12901 agaagcatac cgctggctgc gtgcttagtt gggcgtcgtg ggtctggcgt gcaactgcga
    12961 gaacgcgaga ctgcacagcg caactgtggc gaccgcaatt gcaacgccca tgagtttgcc
    13021 ggccagaaac gggcggtgga gcacgatcac cacggcgaca cgccccgcaa taaatatgag
    13081 cagcgcgacc gcgaattgaa tatagctggg aactacgacg cgcgcatatc tcttcagg
//
LOCUS       DATLVW010000355         4206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_96961, whole genome shotgun sequence.
ACCESSION   DATLVW010000355 DATLVW010000000
VERSION     DATLVW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96961"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VNN25_10800"
     CDS             complement(<1..791)
                     /locus_tag="VNN25_10800"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HXH92059.1"
                     /translation="MTPWLLAARPKTLAAAVTPVLVGTALARNGTAAIHWPSFVFALL
                     GATFIQIGTNYVNDALDFKKGADTHARLGPLRVTQAGLLSADAVLRGAYVCFAIAALC
                     GVPLILRGGWPIAAIGVASIVAAYAYTGGPYPLAYHGLGEVFVMIFFGFVAVCGSFYL
                     QRLTLDPTAWIGGLAVGALAVVILAINNLRDLENDRASNKRTLAARLGARFAFGEIVF
                     FAILPFVCVGAIAYLRGRWTMLIPMLAAVLGFALLLRVARSRGAEL"
     gene            complement(1156..1980)
                     /gene="menB"
                     /locus_tag="VNN25_10805"
     CDS             complement(1156..1980)
                     /gene="menB"
                     /locus_tag="VNN25_10805"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HXH92060.1"
                     /translation="MAELWTSIHEFQDIRYERTADGIAKITINRPEVRNAFRPLTVNE
                     MKQALDMARDDSEIGVIIFTGEGTDAFCSGGDQRVRGKGGYVDPNEKIPRLNILDVQK
                     QIRSMPKPVVAMVAGYAIGGGHVLHVVCDLTIAADNARFGQTGPRVGSFDAGLGSSYL
                     ARIVGQKKAREIWFLCRQYDAQQALDMGLVNTVVPLAQLEEETLKWCREMLALAPTAL
                     RFLKAAMNADCDGQIGLLDFGGDATLLYYLSEEAQEGRDAYVEKRKPDFKKFPRFP"
     gene            complement(2040..4100)
                     /locus_tag="VNN25_10810"
     CDS             complement(2040..4100)
                     /locus_tag="VNN25_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH92061.1"
                     /translation="MRLRLCAVAVMCATSAFAQMRETVNVNVIEVPVSVADSSGNPVR
                     GLTAANFEVYDNGKKQTISSFDKADFASKEVVNAISPMNPAARRSFLLLFDLGFSSPR
                     SLVRAQEAARSFVTTAVHPRDLVGVGTIDNDRSFRLLTAFTTDHDLVAAAIGDPHSFH
                     GKDPLQIGNRTFVNDLIPADDVGMQDAQDASLGAPQKSAAAKIGDAENRETQQRLTAS
                     NEEYVRARVEKQIDYLADLARTLRAVPGRKQVIFLSEGFDAKYVQGRDARDTKANQSD
                     FEKVARGQVWDVDTNQQFGSATSQSILDRMARAFRSSDAVLSAIDIQGVRVENTNSGG
                     ANSAPASLNSNEGLFVLSRPTGGEVFRNTNDLKTNFDRLLHAQEVVYILGFQAQAGNL
                     GKLHDLRVKLVDSPGRVSYRMGYYENGTESAAERALSTAEVIVNDIPQNGVRVAAFAA
                     PFPVSHGNSQVPVILELNGTDLVKQARGNAAGAEIYVYAFDSEGIVRDRLYQRVKLDM
                     QKIGDRLRGSGLRYYGTLVLPPGTYAIKSLVLAGEPDAKRTNDADRRGYARTDVVVPQ
                     AGDVALLPPIPIDEQPKWVLVKGTDRGTTGEYPFELSGQIFIPSATASNKVAVVVYGA
                     AANELTWETIPKTKLLGTIPAMGATKFILQLDDPAPSSSLKVTMHMQGVAEPQTTSVA
                     LVRP"
BASE COUNT          798 a         1290 c         1315 g          803 t
ORIGIN      
        1 agctccgcgc cgcggctgcg tgcgacgcgc agcagaaggg cgaatccgag aacagcggcc
       61 agcatcggta tcagcatcgt ccatcgtccg cgcaggtacg cgatcgcgcc gacgcagacg
      121 aacgggagga tcgcgaagaa aacaatctcg ccgaatgcga agcgcgcccc gaggcgcgcg
      181 gcgagcgttc gcttgttgct ggcccggtcg ttctcgagat cgcgcaagtt gttgatcgcg
      241 aggatcacaa ccgcaagcgc gccaaccgca aggccgccga tccatgcggt cggatcgagc
      301 gtcaaccgtt gcaggtagaa cgatccgcaa accgcgacga agccgaagaa gatcatcacg
      361 aagacttcac cgaggccgtg gtaggcaagg ggataagggc cgccggtgta ggcgtaagca
      421 gcgacgatcg aggcaacgcc gatcgccgca atcggccagc cgcctcgaag aatgagtggg
      481 acgccacaaa gagcggcgat cgcaaagcac acgtacgcgc cacgcagaac ggcatcagcg
      541 ctgagtagcc ccgcctgcgt aacgcgcaac ggaccgaggc gcgcatgcgt atccgcccct
      601 ttcttgaagt cgagcgcatc gttcacgtag ttcgtgccga tctgaatgaa cgtcgcgcca
      661 aggagcgcga agacaaacga cggccagtga atcgcagcgg ttccgtttcg cgccagcgca
      721 gtgccaacaa ggaccggcgt gacggcggca gcgagggtct tggggcgtgc ggcgagaagc
      781 cagggagtca tgatttattg cgcctggcgg cgcgttgtcg tttgtcggtt gtcggttcac
      841 acgagttttc agtgtgtgcc cgaacttgcc agcagtgaaa ttcgagcgcc ttctcccccc
      901 gtgcttttcg gggggagaag gcggatgagg ggggtgttgc attcgacgag cacgcgtgcc
      961 gaggtctgct tacgtgatgg gagtttgcaa ctgccggcgc aagaggcaaa cgccccctca
     1021 tccggccttc ggccaccttc tcccccactg aaaagcgtgg gggagaaggc gctcgattgg
     1081 aggttgtgga ggaattcgca cagaccgcgg gagagacaac cgaaaaccga aaaccaacaa
     1141 cgcgcgcagc gcacatcaag gaaatctcgg gaacttcttg aaatcgggct tgcgcttttc
     1201 tacgtaggcg tcgcggcctt cctgtgcttc ctcggagagg taatagagca gcgtggcgtc
     1261 gccgccgaaa tcgaggaggc cgatctggcc gtcgcagtcg gcgttcatgg cggctttgag
     1321 gaaacgcagc gcagtcggcg cgagcgccag catctcgcgg caccatttca gcgtctcctc
     1381 ctcgagttgc gccagaggca caactgtgtt gaccaggccc atgtcgagag cctgctgcgc
     1441 atcgtactgg cgacagagga accagatctc gcgcgctttc ttctggccga cgattctcgc
     1501 gagatacgac gatccgaggc cggcgtcgaa ggagccgacg cgcggaccgg tctggccgaa
     1561 acgcgcgttg tcggcagcga tggtcagatc gcagacaaca tgcagcacat ggccgccgcc
     1621 gatcgcgtag cctgccacca tggccacgac tggcttcggc atcgaacgga tctgcttctg
     1681 cacatcgagg atattgaggc gcgggatctt ctcgttcgga tcgacgtaac cgcccttgcc
     1741 acgcacgcgt tgatcgccgc cggagcaaaa ggcgtcggta ccttcgcctg tgaaaatgat
     1801 gacgccgatc tcgctgtcgt cgcgagccat atccagcgcc tgcttcatct cgttcaccgt
     1861 cagcggccgg aacgcattgc gcacttcggg acgattgatc gtgatctttg cgatcccgtc
     1921 ggccgtgcgc tcgtagcgga tgtcctgaaa ctcgtgaatg ctcgtccaga gttctgccat
     1981 gcgcggcagg ctatcaagcg cagcgtgatc gatcaacggc ggacgtgctg ctgtgccggc
     2041 tagggacgca ccaatgcgac gctggtcgtc tgcggctccg ctactccctg catgtgcatt
     2101 gtcaccttga gcgacgatga aggcgcggga tcgtcgagct ggaggatgaa cttcgttgcc
     2161 cccatcgcgg ggatcgtgcc caacaatttt gttttaggga tcgtctccca ggtcagctcg
     2221 ttcgccgccg caccgtagac gacgaccgcc actttgttcg atgcggtcgc gctggggatg
     2281 aagatctgtc cgctcagttc gaacggatac tcgcccgtcg tgccgcggtc cgttcccttg
     2341 acgagaaccc actttggctg ttcgtcgatc gggatcggcg gcagcagcgc gacatcgccc
     2401 gcttgcggca cgacgacatc cgtccgcgcg tagccacgcc tgtcggcatc attcgtccgc
     2461 ttcgcgtcag gctcccccgc aaggacgagc gacttgatcg cgtacgttcc cggagggagc
     2521 acaagcgtgc cgtaatagcg aagtccgctg ccgcgaagac gatcgccgat cttctgcatg
     2581 tccagcttga cgcgctggta caagcggtcg cgaacgatcc cctcggaatc gaaagcataa
     2641 acatagattt ccgctcccgc ggcgttaccc cgagcttgct tcaccagatc cgtgccattg
     2701 agctcgagaa tcacaggaac ctgcgagttg ccgtggctga cggggaatgg cgccgcaaac
     2761 gcggcgacgc gcacaccgtt ctgggggatg tcgttgacga tcacctcggc cgtgctgagc
     2821 gcgcgctcgg ctgcagactc ggtcccattc tcgtaatagc ccatccgata cgacacgcgg
     2881 cccggcgagt cgacgagctt gactctcaaa tcgtgcagtt tccccaggtt cccagcctgc
     2941 gcttgaaagc cgaggatgta gaccacttcc tgggcgtgaa gtaagcggtc gaagttggtc
     3001 ttgaggtcat tcgtattgcg gaagacttca ccgccggttg ggcgggaaag gacgaagagt
     3061 ccctcgttcg agttcaggct ggcgggggcg ctgttggcgc cgccggagtt cgtgttctcc
     3121 acgcggacgc cctgaatgtc gattgcgctg agcaccgcgt ccgacgagcg aaaggctcgc
     3181 gccatccggt cgagtatcga ctggctggtc gcgcttccga actgctggtt cgtatcgacg
     3241 tcccaaactt gtcccctggc caccttttcg aagtcgctct ggttggcctt ggtatcgcgg
     3301 gcatcccgtc cctgcacgta tttcgcgtcg aagccttcgg agaggaagat gacctgtttt
     3361 cggcctggaa cggcgcgaag cgttctcgcc agatcggcca ggtagtcaat ctgcttctcg
     3421 acgcgagcgc ggacatactc ctcgttgctg gccgtcaacc gctgttgcgt ttcgcggttt
     3481 tctgcgtcgc ctatttttgc cgcagcgctc ttctgcggcg ctcccagact cgcatcctgg
     3541 gcatcctgca tccccacatc gtcggcagga atcaagtcgt tgacgaacgt ccggttcccg
     3601 atctgcagcg gatcctttcc gtgaaaactg tgcggatcgc caattgccgc ggcgaccagg
     3661 tcatggtcgg tcgtaaacgc tgtgagcaac cggaagctac gatcgttgtc gatggtgccg
     3721 acgccgacga gatcgcgcgg gtgaaccgcg gtggtgacga agctgcgcgc cgcctcctga
     3781 gcccggacca gtgaccgcgg tgatgaaaac ccgaggtcga agagaagcag gaacgagcgc
     3841 cgcgccgccg gattcatcgg cgagatggca ttcactacct ctttcgaagc gaagtccgcc
     3901 ttgtcgaacg aagagatcgt ctgtttcttt ccgttgtcat agacctcgaa gttggccgcc
     3961 gtgaggccgc gcaccggatt tccgctggaa tcggcgaccg acactggcac ctcgatgacg
     4021 tttacgttga cggtttcgcg catctgcgcg aacgcggacg ttgcgcacat cactgccacc
     4081 gcacagagac gcagcctcat cgggtcctcc ttggagaata gccggttagc cacaccatgg
     4141 ccgaaaaacc ccacccgggt ggttacagga cggtggcgtt tgcctgtatc actacgtttg
     4201 ctgttg
//
LOCUS       DATLVW010000356         7440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_183146, whole genome shotgun sequence.
ACCESSION   DATLVW010000356 DATLVW010000000
VERSION     DATLVW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183146"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2272)
                     /locus_tag="VNN25_10815"
     CDS             complement(131..2272)
                     /locus_tag="VNN25_10815"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92062.1"
                     /translation="MKALLLAVTLANPILFVTQVPVPGDFTTVASTFGNHLASAAEAP
                     RGGDLWIRYTDGTTKNLTRGTGFGNGGMQGAGAIAVREPSVHWSGTKALFSMVIGAPS
                     QQYVWESYYWQIYEVTGLGENETAVIRKIPNQPANYNNVSPFYGTDDRILFTSDRPRS
                     GERHLYPQLDEYEEAPTVTGLWSLDPVGGDLRMLDHTPSGLFTPSIDSFGRVIFTRWD
                     HLQRDQQADTDATEGGSYGTFDYADESAGAARLDQRVEIFPEPRSSRTDLLAGTNLEG
                     NSINHFFPWEINEDGTSEETVNHVGRHELFGYFDRSMNDAGLSEFNGDPNRANKNVVE
                     SLFQLHEDPTQPGVYYGVDAPEFQTHASGQIVRFAAEPLRTADNIAITYITPRSTRDV
                     PEGPPPADASGHYRDPLPLADGTLLAVHTSEMRGDDNDGTRAAPKSRYAFRIRTMTQQ
                     GGVYVPGATLTQGIQGSVSYFDPDVLVTWSGEMWELNPVEVRARPRPARRTTLLEEPE
                     AKLFRDENVDPAAFRADLQARNLALIVSRNVTARDGADKQQPYNLHVTGSSTQKVSGS
                     SKLFDIAHLQLFQADQLRGLGGTAAPRDGRRVLARVMHDSAVKNAPTSGPAGSVQIAA
                     DGSIAAFVPAHRAMSWQLTDAAGTPVVRERYWLTFQPGEVRVCSACHAVNSHDQLGAA
                     APTNSPEALRALLRTWKASQTTTPRRRSAGK"
     gene            2488..3885
                     /locus_tag="VNN25_10820"
     CDS             2488..3885
                     /locus_tag="VNN25_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH92063.1"
                     /translation="MDFEYDAFISYSHTDEKWVNEVLLTAFDAGGVKTCIDHRNFAEG
                     RSVPDNFATAITTSRRSVLVLTPGWLASQYTTSEGQMAWGTDPAGWHGRMVPILRTPC
                     KPPRWISARSWLDFIQNNAVIAGMPRLIRLLKSPLSPVVRTDDVIDRLGRLTTGPLRT
                     RALRYLVGLEEVADRTLRVGTLKEVHDELDNLQRHCLHPITTVLVDYDAELLDQPRIE
                     NFHEYAATLARGILKLDGLERTPVFASTGLEFIRKLQAAHDSLTECLATRSVKKARDA
                     TRLMERVMAVHPAVIDARLSEAASQAQLNRVIDALVRMIEESRDMDPAAVADIEIALH
                     ELEELDDNVQELIHTHSLWQDLDIDFRRVESAITPNDITALANDWADLKERATVLSAV
                     VPMIVRSADSYGPAVDIALAGSDARLTISNFRIYKSHAGTRFHALDQTLKDQCAELVK
                     AGASVARLVKRLHDE"
     gene            3878..6583
                     /locus_tag="VNN25_10825"
     CDS             3878..6583
                     /locus_tag="VNN25_10825"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92064.1"
                     /translation="MSDVTTASSSEPEPTLFDLHKQHTELLRAVRQERVKEIAGDVHR
                     FVLRAKDAGTYLFENDQRIAAQGFIDFWTTTLYRAAVPMDEVELAQYDPERAAALECP
                     YAGLKPFSDKQSRFFFGRDQVLNDALDKLRAGRRLIAILGPSGSGKSSLLFAGLIPRL
                     KLGRDLPGSEAWRYLPAIVPGSDPLKALAGALTQDDVPRKRKHRDGLVKPSEGKTPIV
                     VAIDQFEELFTLCEDAGAQEAFAANLVSLATGEGEHIVIVTMRSDHERHLANYPELRE
                     LFTSGGVRVTAMTLAELRDAIERPAREVHLPIEQGLADAIAKDLVTEGAALPLLQFTL
                     RKLFDSRDGQPLRLSMYDEFGGARNALGNAAHQFHESIPLEKRDLVRRVMLRMVRPGS
                     QDETTSNRVRVTTLMEMPGTERADVRYVIDELLRARLIRRTTGETAEDEQVEVAHEAL
                     IRKWPELDAWLERAREDLRELRRYEAWADQWERGGRIGGLLDEEQVEDVTKWLKENDV
                     SDLGVNPSLPELVEASRKYIQRRKSDRRFAIIFLCILTALVIVVGVLLGLAWYRKTLA
                     ERAKLAAEAAQAAAERDKDVADAALKFAAEKAARQRETDLHHQLESLLRTSTEERVAL
                     INKQYVEPLKKENAELKRQLAKPTEAQSGDSTELVQARAALAAAQNKATAIQALYDAR
                     QTFIQEKTHENDELRHQLAAATRAPVYDLFTDRLHINYTVRTTDSGEAAGICCFLKDG
                     GQHFLVSTGEALAGEPGDLVVYRGGSGKEYRVANVWAKVGDGPYTATVARLLSGVRVD
                     EKLPQFATSGRKEQGFRGTGQPRIGAAIKVIGRVSQSEGSIVGFNDTTITIKVLLAPD
                     FKGAPVVIGDRHLIGFVSAIEAEKAIVTRIDPIFSVEHEGWELYE"
     gene            6982..7182
                     /locus_tag="VNN25_10830"
     CDS             6982..7182
                     /locus_tag="VNN25_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92065.1"
                     /translation="MPSVRGTVKGSTINLSLRNGFIACTYTLDGTGTVSNGVIRATLT
                     GPTSGPCCGSGIDTVELVATRR"
BASE COUNT         1409 a         2248 c         2427 g         1356 t
ORIGIN      
        1 tccacggcga gagggtacaa ccgcgtaccc aggcctcccg caagaatgac cacttgcgta
       61 cggcgcattt gggtacatcc taactcatcg ctctcggcca atcagtagcg cgtatcacgt
      121 cacgacaacc tcacttcccc gccgatcggc gtcgtggcgt cgtcgtctgc gatgccttcc
      181 atgttcgcag cagcgctcgc aacgcctcgg gggaattcgt cggggcagcg gcgccgagtt
      241 ggtcgtggga gttgacggcg tggcaggcgc tgcagacgcg gacttcgccg ggctggaacg
      301 tcagccagta gcgctcgcgg acgaccggtg ttccggcggc gtccgtcagt tgccagctca
      361 tagcgcgatg cgcggggacg aatgcggcga tcgatccgtc tgcggcgatc tgtacgctgc
      421 cggcgggccc ggaggttggc gcgttcttta ccgcggagtc gtgcatcact cgggcgagca
      481 cccggcggcc atcgcgcggc gctgcggtgc cgccgaggcc gcgaagctgg tcggcctgga
      541 agagttggag gtgggcgatg tcgaacagct tgctgctccc cgacaccttc tgcgtcgagc
      601 tgccggtgac gtgaaggttg tagggctgct gtttgtccgc cccatcgcgc gcggtcacgt
      661 tgcggctgac gatcagcgcg agattgcgcg cctgcaggtc ggcgcgaaag gcggcggggt
      721 cgacgttctc gtcgcggaag agcttcgcct ccggctcctc gagcagcgtc gtccgtcgcg
      781 caggacgcgg gcgcgcgcgc acctcgaccg gattcagctc ccacatttcg cccgaccagg
      841 tcacgaggac gtcgggatcg aagtacgaaa cacttccctg tatgccctgc gtcagcgtcg
      901 ctcccggcac gtagacgccg ccctgttgcg tcatcgtccg gatgcggaaa gcgtatcgcg
      961 acttcggcgc ggcacgtgtg ccgtcgttgt cgtcgccgcg catctcgctc gtgtggaccg
     1021 cgaggagcgt gccgtccgcg agaggcagcg gatcgcgata atgccccgag gcgtcggctg
     1081 gcggcggtcc ctctggaacg tcgcgagtgg agcgaggcgt gatgtaggta atggcgatgt
     1141 tgtcggcggt tcgcaacggc tccgcggcga agcggacgat ctgtcccgag gcgtgggtct
     1201 ggaactccgg cgcatcgacg ccgtagtaga cgcccggctg cgtcgggtcc tcgtgcaatt
     1261 gaaaaaggct ttcgacgacg ttcttgttcg cccgattggg gtcgccgttg aactcggaaa
     1321 ggccggcgtc gttcatgctc cggtcgaagt atccgaagag ctcgtggcgc cctacatggt
     1381 tcaccgtctc ctcggatgtc ccatcttcgt tgatctccca cgggaagaag tggttgatgc
     1441 tgttcccctc gagattggtg ccggcgagaa gatcggtgcg gctgctgcgc ggctcgggaa
     1501 agatctcgac gcgctgatcg agacgcgcgg cgcccgctga ttcgtcggcg tagtcgaagg
     1561 tcccatacga gccgccttcc gtcgcgtcgg tatcggcctg ctggtcgcgc tgcaggtgat
     1621 cccatcgcgt gaagatcaca cgcccgaagc tgtcgatcga cggcgtgaaa agccccgacg
     1681 gcgtgtgatc gagcatccgc agatcgccgc cgacaggatc gaggctccag agtccggtga
     1741 cggtcggcgc ttcttcgtac tcatcgagct gcggatagag atgccgttcg ccactgcgcg
     1801 gacggtcgga agtaaagagg atgcggtcgt cggtgccgta gaacggactg acgttgttgt
     1861 agttggccgg ctgattggga atcttgcgaa tgactgccgt ttcgttctcg ccgaggccgg
     1921 tgacttcgta gatctgccag tagtaggact cccaaacgta ctgctgcgaa ggcgcgccga
     1981 tgaccatgct gaagagcgcc ttcgtcccgc tccagtgcac cgacggctcg cgcaccgcga
     2041 tcgcccccgc cccctgcatc cccccgttcc cgaaccccgt cccgcgcgtg aggttcttcg
     2101 tcgtcccatc ggtatatcgg atccaaagat caccgccgcg gggagcctcc gccgccgagg
     2161 ccagatggtt cccaaatgtc gacgcgacgg tggtgaagtc gcccggaacg ggaacctgcg
     2221 tcacgaagag aatgggattg gcaagcgtga cagcaaggag gagggctttc atgggggttt
     2281 cattttgcgc gggcggaggg cggggggacg tgcgctgggt cacggaattg ccgggtcaac
     2341 agaaacgcga agatcagaca cttgccgagg ccctccttct ccgagcgttc actctgcggc
     2401 taatcgcggc gccgctcgta gcgagggtgg cgattcggca gggatgaacc tatattgcgc
     2461 ccatacagca atttttaagg ggttgcgatg gacttcgaat acgacgcgtt catcagctac
     2521 agccataccg acgagaaatg ggtgaacgaa gtcctgctga cggctttcga tgccggcggc
     2581 gtgaagacct gcatcgacca ccgcaatttc gccgaagggc gttcggtacc ggataacttc
     2641 gccaccgcga tcacaacgtc gcggcgctcg gtgctggtgc tcacgccggg atggttggcc
     2701 agtcagtaca ctacatccga ggggcagatg gcttggggca ccgaccccgc gggatggcac
     2761 gggagaatgg ttccgatcct gcggacgccg tgcaaaccgc cgagatggat ctccgcccgc
     2821 tcctggctcg atttcatcca gaacaacgcc gtcattgcgg gcatgccgcg actaatccgc
     2881 cttctgaaat cgccgctttc tccggtcgtt cgcaccgatg acgtcattga ccggctcggc
     2941 aggctgacaa ccggcccctt gcgcacccga gcactgcgat acctggttgg tctggaggaa
     3001 gtcgccgacc gcacgttgcg cgtggggaca ctgaaagaag ttcacgacga gctcgacaat
     3061 ctccaacgcc actgcctcca tccgatcacc acggtgcttg tcgactacga cgcggaactg
     3121 ctggatcagc caagaataga gaattttcac gagtatgccg ccacgttagc gcgcggcatt
     3181 ctgaaactgg acggtctgga gaggacgccg gttttcgcca gtacaggatt ggagtttatt
     3241 aggaagttgc aggccgcaca cgacagcctg accgagtgtc tggccacccg cagcgtcaag
     3301 aaggcccggg atgcgacgcg gctaatggaa cgagtcatgg ccgtgcatcc ggccgtgatc
     3361 gatgcgcgtc ttagcgaagc ggctagtcag gcgcagctga accgcgtgat cgacgcgctc
     3421 gtccggatga tcgaggagtc gcgcgatatg gatccggcgg ccgtggcgga tatcgaaatc
     3481 gcgctccatg agctggaaga gctcgacgac aacgtgcaag agcttataca cacccacagt
     3541 ctctggcagg acctcgatat cgacttccgg cgagtcgagt cagcaatcac gcccaatgac
     3601 attacggcgc ttgcgaatga ctgggccgat ctcaaggaac gggccaccgt tctttcggca
     3661 gtcgtgccaa tgatcgttcg aagcgccgac agctacggcc ccgcggtcga tatcgcgcta
     3721 gccggcagtg acgccaggct gaccatctcg aatttccgaa tctacaaatc gcacgcaggc
     3781 acgcgtttcc acgcactcga ccaaaccctg aaggatcagt gcgcggagct ggtaaaggca
     3841 ggcgcttccg tcgctcgtct cgttaagagg ctgcatgatg agtgacgtca ccaccgcgtc
     3901 cagttctgag cctgaaccga cgctcttcga cctccacaag cagcatacgg aactgcttcg
     3961 cgccgtaaga caggagcgcg taaaggagat cgcgggcgac gtgcaccgct ttgttctccg
     4021 cgcgaaagat gccggcacgt atctgttcga gaacgaccaa cgcatcgcgg cgcagggatt
     4081 catcgatttc tggaccacca cactctatcg cgccgccgtg cctatggatg aagtagagct
     4141 cgcgcaatac gatcccgaaa gagctgcggc gctggagtgt ccgtacgccg gtcttaagcc
     4201 gttcagcgat aagcaatcgc gtttcttctt cggccgcgat caagtgctca acgacgcgct
     4261 cgacaagctc cgcgccggcc gccgtctcat cgccatcctc gggccgtcag gaagcggcaa
     4321 gtcgtcgctg ttgttcgctg gactcattcc ccgcctcaag ctcggccgcg atctgcccgg
     4381 aagcgaggcg tggcgctatc tcccggccat cgtccccgga tccgatccgt taaaagctct
     4441 ggccggagcg ctgacgcagg acgacgtgcc gcggaagcgc aaacaccgcg acggactggt
     4501 gaagccgtcc gaaggcaaaa caccgatcgt cgtggcgatt gatcagttcg aggagctgtt
     4561 cacgctctgc gaggatgcgg gggcgcagga agcgttcgcg gcgaatctcg tgtcgctggc
     4621 gacgggcgaa ggtgagcaca ttgtcatcgt cacaatgcgc tcggatcacg agcggcatct
     4681 ggcaaactac ccggaattgc gcgagctctt cacgagtggc ggcgtacgcg tcacggcgat
     4741 gacgctcgcc gagttgcgcg atgccatcga gcgtccggcg cgggaagtcc atcttccgat
     4801 agaacagggg ctggccgatg cgatcgccaa ggatctggtc accgaagggg cggcattgcc
     4861 gctactccag ttcacccttc gcaagctgtt cgattcgcgc gacggtcagc cgctgcgcct
     4921 atcgatgtat gacgagtttg gaggagcccg caacgcactg ggcaatgccg cgcatcagtt
     4981 ccacgagtcg ataccgctag agaagcgcga tctcgtgcgt cgcgtgatgc ttcggatggt
     5041 gcggcccgga tcgcaggacg agaccaccag taatcgggtt cgtgtcacga cgctgatgga
     5101 gatgccgggc actgagcgcg ccgacgttcg ttacgtcatc gacgagctcc ttcgcgcgcg
     5161 ccttattcga aggaccaccg gcgagacggc ggaggacgag caggttgagg tcgcacacga
     5221 ggcgctcatc cgcaaatggc cggagctcga cgcttggctg gagcgggcgc gcgaggatct
     5281 gcgcgagttg cgccgctatg aggcgtgggc ggaccagtgg gagcgcggtg gccgcatcgg
     5341 cgggctgctc gatgaagagc aggttgagga tgtgacgaag tggcttaagg aaaatgacgt
     5401 cagcgacctc ggcgtgaacc cttcactgcc ggaactcgtc gaggcaagca ggaagtatat
     5461 ccaacgacgg aagtccgatc ggcggttcgc gatcatcttc ctgtgcatcc tgaccgcgtt
     5521 ggtgatcgtg gttggcgttc tcctcggctt ggcgtggtac cggaagaccc ttgctgagcg
     5581 cgcgaagctg gcggccgagg cggcgcaagc ggcggccgag agggacaagg acgttgccga
     5641 cgcagccctg aaattcgcag ccgagaaagc ggcgcggcaa cgcgaaaccg atctgcatca
     5701 tcagttggag agtctgctca ggacgtcgac ggaagagcgg gtcgctctca taaacaagca
     5761 gtacgtcgaa ccgctcaaga aagagaatgc tgagttgaaa aggcagctcg cgaagccgac
     5821 cgaagctcaa tcgggcgata gcacggagct tgtgcaagcg cgcgccgcgc tggcggcggc
     5881 gcagaacaag gccacggcaa ttcaggcact gtacgacgcg cggcagacat tcattcaaga
     5941 gaaaacccac gagaacgacg agctgcgcca tcaactggcg gcagcgacga gagcacccgt
     6001 ctacgaccta ttcaccgacc gcttgcacat caactacacc gtgcgtacca ccgacagcgg
     6061 cgaggccgcc ggaatctgct gcttcctcaa ggatggcggg caacacttcc tcgtctccac
     6121 tggtgaagca ttggccggag agccgggtga cctagttgtc taccgcggcg ggagcggaaa
     6181 ggaataccgg gttgcgaatg tctgggcgaa ggtgggcgac ggtccgtaca cagcgaccgt
     6241 ggcgcggcta ctctccggag ttcgcgtcga cgagaagctg ccgcagttcg ccacctccgg
     6301 acgcaaggag cagggattcc gtggaaccgg ccagccgcga ataggcgcgg ccatcaaagt
     6361 gatcggacgg gtgtcgcagt ccgaggggag catcgttggg ttcaacgaca cgacgatcac
     6421 tatcaaggtg ctgctcgccc ctgacttcaa aggcgctccg gtggtcatcg gagaccgcca
     6481 cctgatcggc ttcgtctccg cgatcgaggc ggagaaggcg atcgtcacgc gtatcgaccc
     6541 gatcttcagc gtcgaacacg aaggatggga gctctacgaa taggcgcttc gctatgccac
     6601 cgtcgtcacg ttcgttcgtg gcgccgttcg agtaaagcac gcccgcctcg aagaagagtt
     6661 agccagtagt cgcccctcgg cgcgcgtatc attgtcttcg aaatgaaagc gctcccagtt
     6721 gtcgctgtag ctctgctctt cgccggttgc tcacaggagt tgccgacctc tccgattcaa
     6781 tcggcgcaga gcgcgcgtcc gctaacgacc gaggccactc gcgtgggacg gggtcgcgcg
     6841 gttgtcccgc ccgcggggtg tgtcgatgtt tcggggatct atgatgtggt gtacgacggg
     6901 cattgtccta ccagtggata cttgacgagc tgggagctcc ggcagaatgg atgcgcgttc
     6961 gttgcgaata tcaatcctga cttgccgtcg gtacgaggga ctgtgaaggg atcgacgatc
     7021 aacctttcgc tgcggaatgg cttcatcgcg tgtacgtaca ccctcgacgg cacgggcacc
     7081 gtatcgaacg gtgtgatcag ggcaacgctg accggtccga cttccggtcc atgttgcggg
     7141 tcggggatcg acactgtgga gctcgtggcg acgagacgct agcggaatca actgcgaagg
     7201 cgggttcggc ggcagcagtg cggtctccag cctccaccgt aatttgctga agagcgttcc
     7261 gggccgccgc gcgatcgtgc gcgattcacg atcggggcga taatgaaacg ctccggaaac
     7321 actgtaccgc ctgcatatgt cctcgaagac ttcaggtaga cgcagcgacg cgcgagaccg
     7381 gctgctcgct cacgtcgcct tcgatgccat ctgggagaga gatctccaaa gcggcgcggt
//
LOCUS       DATLVW010000357         5166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_14550, whole genome shotgun sequence.
ACCESSION   DATLVW010000357 DATLVW010000000
VERSION     DATLVW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14550"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="VNN25_10835"
     CDS             complement(<1..2071)
                     /locus_tag="VNN25_10835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH92066.1"
                     /translation="MHDVAIFVGGDGRIIEVNAAAVKTYCRSAEELVGSPAAELCYRG
                     DRCAIQRTLETGEPRTCECVHRRADGTLLPLEVSTVRADIGGEHLVLCVARDVAHRRR
                     RELLQAIVREIDGAILAGQPQETTLEFICSQLVSLNGYALVQISIKERDGLVSIQGSA
                     GAAAGFLDDIHVRWDSGSESLGPTGRAIREGSVQICDIENDPDFEVWRSSAAAHGLRS
                     SISLPLAAHGQTLGALTLFSTEKHAFTDAMNDEMGSFADEIALSILAGMGHAKIRLQR
                     VALEAAANAIVITDVHGVIEWVNPAFTTLTGWTAEEAVGDTPRMLRSGNHSRAFYTRM
                     WQQLNNGEVWRGEMFNRRKDGTIYPEEQTITPVRDEKGRIAHFVAIKQDISDRKRQEE
                     QIRFLALHDVLTALPNRRAFEATLARFTHDANTARNAAILVFDVDEFKLVNDSAGHPV
                     GDELLIELTSLLQAQLRPGDFLARLGGDEFVVLLRNVGKNLAHDIAERLRRAVEAMWF
                     EHDGIVYTITISIGIAMLAEGDDPKDLVAHADMAMYSAKEHGKNRVASYPLAKGEVVH
                     EVTRWLAKVRSSLRDGSFILTFQPIVQLGNGETLHFEALIRMRAEDGSIIPPDNFLPS
                     AERYGLMPQIDRWVFDEVVRILESEASLRIFMNLSARSLNDEPLLQHIEERLRISSIE
                     PGRLSFEI"
     gene            2705..3046
                     /locus_tag="VNN25_10840"
     CDS             2705..3046
                     /locus_tag="VNN25_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92067.1"
                     /translation="MLPHRLSAAILCALLASGAILGGIPSCGRSATSAGPACCRSEAT
                     CPMHQKGALGLNACHRDANNAITVTTHRAVLASAVGAIEIAPDDQTFEATLHVPIAVS
                     LIPSTPPPRRG"
     gene            3093..4076
                     /locus_tag="VNN25_10845"
     CDS             3093..4076
                     /locus_tag="VNN25_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92068.1"
                     /translation="MRKGSWFGALFLLFSFTAGSAVASCGSSSCPIDLHALQFGDTGR
                     FSLDLSFQYIDQDQPRIGSRRAHVGEIASDHDEIRTINRLATLQLSYAMNDRFQVALA
                     LPYVSRSHEHFDEETAQIERWNFADPGDATLQARLRLFSDAPTHSSVWLSGGAKFATG
                     SRHEIGSSGEDAEVTITPGNGSTDALLGVTYQSAFVRDTKLEGPMGHATLIPFFVALN
                     GRVNGRGTGDYRRGHEIQLNAGTEYPLTQAVHLLGQLNGRMTSKDDVGSTDENRNLTG
                     GRYLYLSPGLRVAMSHGTSIYGFVQLPIYQHVNGIQLTSKANYVAGVRRSF"
     gene            4251..>5166
                     /locus_tag="VNN25_10850"
     CDS             4251..>5166
                     /locus_tag="VNN25_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92069.1"
                     /translation="MSSNRSWFARLFAVITLALAVSAIPLFAAPPLPGAIFTTDGACT
                     GTDLNIYGSKLDVFVNGGPAHPGAASLPDGTYFLRVTEPNGALLGNPPRDGSNNPIAK
                     VTVAGGTFVQCYNLSANVVKQSDGSAGYDDTTNPGGEYKVWISTTADFINSSTKTDNF
                     KVKAAPVVNPGHLHVLKFYDANANGQYDSGETLITGWQVAIADQEINWFDHSWIDYIR
                     STPVDLVGDSGTYHAGEAMPNEANWRFIAATAADGSMVLTNHVDTPITDGDDKTIRFG
                     NLCLGAGGGLTLGYWSNKNGQASFNNIPN"
BASE COUNT          962 a         1619 c         1582 g         1003 t
ORIGIN      
        1 tgatctcgaa ggaaagcctg cccggctcaa tgctgctgat gcgaaggcgc tcctcgatgt
       61 gctgcagcag aggttcatca ttgagacttc gcgcggagag attcatgaag atgcgcaagc
      121 tcgcttccga ctcgaggatg cgcacgactt cgtcgaagac ccagcggtcg atctgcggca
      181 tcagtccgta gcgctccgcg gaggggagga agttgtcggg cggaatgatg ctgccatcct
      241 cggcgcgcat ccggatcagc gcttcgaagt ggagcgtctc gccgttgccg agttgaacga
      301 tcggctgaaa ggtgaggatg aagctgccgt cgcgaagaga cgatcggact ttcgccagcc
      361 agcgcgtgac ttcgtggacg acctcacctt tggcgagcgg atagctggcc acgcgatttt
      421 tgccgtgttc cttcgcggag tacatcgcca tatccgcatg cgcgacgagg tccttcggat
      481 catcgccctc ggcgagcatg gcgattccga tgctgatcgt aatggtgtag acgatcccgt
      541 cgtgctcgaa ccacatcgct tctaccgcgc gccgcagccg ctcggcaatg tcgtgggcga
      601 gattctttcc gacgttccgc agcagaacga cgaactcgtc gccaccgagg cgggcgagaa
      661 aatcgccggg ccggagttgc gcctggagca atgatgtgag ctcgatgagc aactcgtcgc
      721 cgaccgggtg tccggctgag tcgttgacga gcttgaactc atcgacatcg aagacgagga
      781 tggcagcatt gcgcgcggta ttggcgtcgt gtgtgaaccg cgccagcgtg gcctcgaatg
      841 cgcggcggtt ggggagcgca gtgagaacgt cgtgcagggc gagaaagcgg atctgctctt
      901 cctgccgctt ccggtcgctg atgtcctgct tgatagccac gaaatgcgcg atccgccctt
      961 tctcgtcgcg taccggtgtg atggtctgtt cctcgggata gatcgttccg tctttgcgcc
     1021 ggttgaacat ctcgccgcgc cagacttcac cattgttcag ttgctgccac atccgcgtgt
     1081 agaacgcgcg gctgtgattt cccgatcgca gcatgcgcgg cgtgtcgccg accgcttctt
     1141 ccgccgtcca gccggtcagc gtcgtgaagg cgggattcac ccattcgatc acgccgtgta
     1201 cgtccgtgat gacgatggca ttcgcggccg cctcgagcgc gacacgctgc aggcggatct
     1261 tggcgtgtcc catgccggcg agaatcgaca gcgcgatctc atcggcgaag ctccccatct
     1321 catcgttcat cgcgtcggtg aaggcatgct tctctgtcga aaatagcgtc agcgctccga
     1381 gcgtctggcc gtgcgccgcg agagggagcg agatcgatga ccgaagccca tgcgccgcgg
     1441 cgctgcttcg ccagacttcg aaatcaggat cgttctcgat gtcgcagatc tgaacgctcc
     1501 cctcgcgaat tgcccgtccg gtggggccga gggattcgga gccgctatcc cagcgaacgt
     1561 gaatgtcgtc gaggaagccg gcagccgcgc cggcggagcc ctgaatggag acgaggccgt
     1621 cgcgctcctt gatgctgatc tgtacgagcg cgtagccgtt gagcgagacg agctgcgagc
     1681 agatgaactc cagcgtggtc tcctgcggtt gtccggccag gatcgcgccg tcgatctcgc
     1741 ggacgatggc ctgcaggagc tcgcggcggc gccggtgggc cacatcgcgc gccacgcaca
     1801 agacgagatg ctcgccgccg atatcggccc gaacggtact cacctcgaga gggaggagcg
     1861 tcccatccgc acggcgatgc acgcattcgc aagtgcgcgg ttccccggtt tcgagcgtgc
     1921 gctggatcgc gcagcggtcg cccctgtagc agagctccgc cgcggggctc ccgacaagtt
     1981 cctccgcgct cctgcagtac gttttgacag cagcggcatt cacctcgatg atcctgccgt
     2041 ccccgccaac gaagatcgcc acgtcatgca tggtctcggc gagaagacgg tatttgctca
     2101 ggacgagcgc gctatcggtc acaccgggga tggtgatggg agcgcgcgca tgaacgcatg
     2161 atgaggatca cgtgcgggtg tgatgggcga tctctattac tttctagttg caccaccagc
     2221 cttgcttttt gcgacgctcc ccaaccgagg tgccgaaggc gggggaacgc gcacggcgat
     2281 caacaacgcc tcgtccgaac tgcttgcgtg tcatacggac gtatgtcgcg ctcgggaacg
     2341 ccccctcatc cggcttcgcc accttctccc ccactgaaaa gcgtggggga gaaggcgctc
     2401 gattgaaagg ttgtgatgag agtgggagaa agagagatgg ctcggggata gctagcattt
     2461 ctcgctcgcg ttgaccgtcc tgctgcgctc ttgcaacacc acctcttatt gtcctttctg
     2521 ctgcatcttt ctctatatgt tttgcgacgc tctccaatcg agcgccttct ccccccgcgg
     2581 tttttgcggg gggagaaggt gccgaaggcg gatgaggggg gcaaatatgc aggagcgacg
     2641 aactccccta ttcgatcgga aaggttgcga tcagagagaa ttgtccgggt tagaatccct
     2701 tcccatgttg ccgcatcgcc tctcggccgc gatcctctgc gcgctccttg cgagtggcgc
     2761 gatcctcggc gggattccgt cgtgtgggcg gagtgcgaca tcggccgggc cggcgtgctg
     2821 caggagcgag gcgacgtgtc cgatgcatca aaagggtgcg ctcggactca acgcatgtca
     2881 ccgcgatgcg aacaatgcca tcaccgtaac cacgcaccgc gcggttctgg ccagtgctgt
     2941 cggcgcgatt gagattgcgc cggacgatca gaccttcgaa gccacgctgc acgtcccgat
     3001 cgcagtgtcg ctgatccctt ccacgccccc tcctcggcgc ggctgacgtc ccctacgtca
     3061 cctgatcgtt tacgaccccg aggaggtttc ctttgcggaa aggctcttgg tttggagcgc
     3121 tgttccttct gttttcattc accgccggaa gcgcggttgc gagctgcggc tcgtcatcgt
     3181 gtccgatcga tctccacgca ctgcagttcg gcgataccgg ccgtttttcg ctcgatctgt
     3241 cgttccagta catcgatcag gatcagcctc gcatcggcag ccggcgcgcg catgtgggcg
     3301 agatcgcctc cgatcatgac gagattcgca cgatcaatcg tcttgccacg ctgcaactga
     3361 gctacgcgat gaacgatcgc tttcaggtcg cgctggcgct tccctatgtc tcgcggtcgc
     3421 atgagcattt cgacgaagag accgcgcaga tcgagcgctg gaacttcgcc gatcccggtg
     3481 acgccacgct gcaggcgcgc cttcgtttgt tcagcgatgc accaacacat tcgtcggtct
     3541 ggctcagcgg cggcgcgaag ttcgcgaccg ggtcgcgcca tgagatcggg agcagcggcg
     3601 aggatgccga ggtgacgatc acacccggaa acggctccac cgacgcgctc ctcggcgtca
     3661 cctaccaatc cgcgttcgtg cgcgacacga aactggaagg gccgatgggc cacgcaacgc
     3721 tcatcccgtt cttcgttgcc ttgaatggcc gtgtgaatgg ccgagggacc ggcgactatc
     3781 gccgcgggca tgagatccag ctcaacgccg gaaccgaata cccgctcacg caagcagttc
     3841 atctcctcgg ccagttgaac ggccggatga cgagtaagga tgatgtcggt tcgaccgatg
     3901 agaaccgcaa cctgaccggc ggccgctacc tctatctcag ccccggcctg cgcgtggcga
     3961 tgagccacgg aacctctatc tacggattcg tgcagctccc gatctaccag cacgtgaacg
     4021 ggatccagtt gacgtcgaag gcgaactacg tggcgggcgt cagaagaagt ttctgacgcc
     4081 tccgcgatct ccgcgtctcc gcgtgaaaac gtcccccttc ttccctgcca gacgaaaaaa
     4141 gatcttccga tcgtggaaaa gccgcgcgct ccggccctct tctccgatgt aagcagcaac
     4201 cagagaggct cacttacatc ctggtccgag gaaagaagag gagaacgcaa atgtcatcca
     4261 atcggtcgtg gtttgctcgt ttgttcgcag tcattactct cgcactggct gtttcggcga
     4321 tcccgctctt cgcggcaccg ccgctgccgg gcgcaatttt cacgacggac ggcgcctgca
     4381 ccggcacgga cctgaacatc tatggatcga agctggacgt cttcgtcaac ggcggtccgg
     4441 cccatccggg tgctgccagc cttcccgacg gaacgtactt cctgcgcgtc acggagccga
     4501 acggcgcgct gctcggcaat ccaccgcgcg acggaagcaa caatcccatc gccaaggtga
     4561 cggtagccgg cggcacattc gtgcagtgct acaacctctc ggccaacgtg gtgaagcagt
     4621 ccgacggcag cgccggctac gacgacacca cgaatcccgg cggcgagtac aaagtgtgga
     4681 tcagcaccac cgcggacttc atcaacagct ccacgaagac cgacaacttc aaggtcaagg
     4741 ccgctccggt cgtcaatccc ggccatctgc atgtcctcaa gttctatgac gcgaacgcga
     4801 atggtcagta cgacagcggc gaaacgctca tcaccggctg gcaggttgcc attgccgacc
     4861 aggagatcaa ctggttcgat cacagttgga tcgattacat ccgctccaca ccggtcgatc
     4921 tcgtcggcga ttccggcacg tatcacgccg gcgaagcgat gccgaatgag gcgaactgga
     4981 gattcattgc ggccaccgcc gcggacggca gcatggtcct tacgaatcac gtcgatacgc
     5041 cgatcaccga cggcgacgac aagacgatcc gtttcggaaa cctgtgcctc ggcgccggtg
     5101 gtggattgac gctcggctac tggtcgaaca agaacggcca ggcgtcgttc aacaacatcc
     5161 cgaacg
//
LOCUS       DATLVW010000358        13359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_349149, whole genome shotgun sequence.
ACCESSION   DATLVW010000358 DATLVW010000000
VERSION     DATLVW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349149"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VNN25_10855"
     CDS             complement(<1..257)
                     /locus_tag="VNN25_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HXH92070.1"
                     /translation="MHPPQLFEANLDVIERAITQVARQVRLRGADAEDFASSARIALL
                     ADDCAVLRKFEGRSSFASYVAIVVRRLFLDQKRSEGRWYPSA"
     gene            complement(479..1861)
                     /locus_tag="VNN25_10860"
     CDS             complement(479..1861)
                     /locus_tag="VNN25_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXH92071.1"
                     /translation="MAAPLSASLRRDLGTIESYATLLGMLIGAGIFKVTSDAWARTGP
                     SVILGYLVLAPAILATSVAYSVFLSTPLGREPGGEYTHLSRTFGGYGLAFVGVWLKVI
                     SYIGALAYLSRALGDYTVELFAGALPKSAAMPVAIGSLLFFYVIHVAGVRWFGRIQVW
                     MCVLLGLSILVLVGPGLFAIHSHNYQPFFTHGLGGFAAALPLVFFGYAGFESLAQTAG
                     EVRDSTRRLPRIFLRGILATTVIYFLMSVVAFGVLPGAELRQAAAPMAAVASRYLPMG
                     AAWFVTLGAVMALTTSVNATMLVPSRVGIMLAEDGLAPQVIGAISKRTGTPIVGLTLT
                     LIVTLLLLVSGQVSLALNIAVFALVCLYFLHSLALLALPRANPSLYASVTLGLPRWVQ
                     QISATISLLFMGTLIAVQLRGDVDVLTHLSFRERIEHASLTTIELVVFWGAIGAALYA
                     IAARGRRVAT"
     gene            complement(1865..2824)
                     /locus_tag="VNN25_10865"
     CDS             complement(1865..2824)
                     /locus_tag="VNN25_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92072.1"
                     /translation="MKRWLRVLAPLAGLVAVILLFAILTGSPARYLSGANLRVVLAQT
                     VIVAIGALGMTMIIVSGGIDLSVGAVIALTSVVTALALHDGWPPIAALLAGIIAGGIV
                     GIVNGLAITALRVVPFIATLGMLGIARGVAKYVAGEQTVNPPATWLNELAVTFPSRDW
                     MLFAPGVWIAIILAAMIALVLRNTVFGRHVFALGSNEAAARACGINTSALKVRIYALA
                     GLFFGLAGVMQMSRLRQGDPTVAVGVELDVIAAVVIGGASLSGGEGSIAGAMIGALVM
                     AFLRNGSQQMGWPNYVQEIIIGVVIVLAVAADRWRASRAAERN"
     gene            complement(3199..4734)
                     /locus_tag="VNN25_10870"
     CDS             complement(3199..4734)
                     /locus_tag="VNN25_10870"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXH92073.1"
                     /translation="MRTTSAGVSRASSPALRLHAITKHFGATIALDGVDLEVAPGEVH
                     ALIGENGAGKSTLMNVLSGAVAPDGGSMIVDGMPYAPRGPADARGRGVAHIHQELALC
                     GHLSVAENIAMGVEPSRGGWLRRDLMNERAATLLAEFGRSEIKPSMRVASLSLADRQV
                     VEICRALARDARILLMDEPTSSLQRADVERLFETVRRLRDRGIAIIYISHFLEEVREI
                     ASRFTVLRDGRSIGSGELTEVRDEELISMMVGAQTRVSVPHQAAHEPRANLQPEVLLE
                     VENLSAPPRLRVASFLLHRGEILGIAGLVGSGRSDLVRALFGLQRATGGIVRLRGKEL
                     KTLAHQIRNGFGYLSEDRKGEGLALQLPIADNVTMSRFATCSRGGWIDRRQQNAQTDD
                     VVTRLRVKAPGPRGIVGKLSGGNQQKVALARLLHQDPDTLLLDEPARGIDIGSKADIY
                     REIARLASEGKGIVMVSSYLPELLSVCDSIAVMTRGRLSAAKPASEWTADTILQAAIG
                     AEM"
     gene            complement(4734..5735)
                     /locus_tag="VNN25_10875"
     CDS             complement(4734..5735)
                     /locus_tag="VNN25_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXH92074.1"
                     /translation="MKRIMIAAGIAMVVIGCAKHEQTTAAKSAKSFTVAVIPQGSTHE
                     FWKSIHAGAMKTAQDEGKAGVNVTIIWKGPMREDDREQQVQVVEGFVTQHVDGIVLAP
                     FDKNALVRPVEEAKRAGIPTVVVDSGLESTDPISFVASNNYHGGELAADEMGRLLGGK
                     GKVLALRYQEGVFSTEQREKGFIERIKAAYPNIQLISSNQFAGATRDTAKTAAENLLN
                     RFGNDLDGLFTPNESSTAGALLALEDAGKAGKIRFIGFDTSEVFVKAMRDGKLQGIVV
                     QNPFRMGELGVKTVVDHLLGNAVEKRVDTGVTLITPTNLDAQESQTLLHPPLETYLK"
     gene            complement(5770..9144)
                     /locus_tag="VNN25_10880"
     CDS             complement(5770..9144)
                     /locus_tag="VNN25_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXH92075.1"
                     /translation="MKKLGAMIALLFACTAVAQAADLPDLTKQPTLYVVGYAHLDTQW
                     RWEYPQTIKEFLPRTMHDNFALFEKYPHYIFNFSGANRYRMMREYYPADYAVLQEWVR
                     KGRWFPAGSSMEESDVNSPSAESIIRQVLYGNAYFRRELGRTSAEYMLPDCFGFPASL
                     PSILHHMGIVGFSTQKLTWGSSAPVGGPDSPEKTPVGTPFNVGVWEGPDGNSVLAAFN
                     PGSYSADIRYDLSRSDTTPPNGVDWPKRVERNGAASGVFTDYHYYGTGDVGGAPREPS
                     VQLVESIVSKNDGPLHVISSTAEEMFLDIKPSQRAGLPRYKGELELTNHSAGSLTSEA
                     YQKRWNHKNELLADAAERASVAAEWLGGRAYPMKRLNDAWTLVMGGQFHDIAAGTATP
                     KAYEYSWNDDVIAMNQFAGVVTSATEAVASALDTRVKGTAIVVYNPLSIAREDVVEAH
                     VSLPENEDVSVFGPDGRAVRSQRDGDTVIFVAKVPSVGWAVYDVRDAGVGSRGSGVGA
                     GRESALRAQLRPPTPDSRQARATLDNARYRVSIDEAGDLASIFDKKTKREVLSAPARL
                     ALQTENPHDWPAWNMDWADQQKPPRGYVSGPATMRIVENGPARAALEVTRETEGSRFV
                     QTIRLATGDGGNRIEIDNAIDWKTEAAALKATFPLTASNKLATYNWEVGTIERATNDE
                     KKFEVASHQWFDLGDAKGGVTVLTDSKYGSDKPDDHTLRLTLLYTPGLSANGRAYSDQ
                     ASQDWGHHEFVYGLAVHAGDWRSEGTDWQALRLSQPLIAFVSPKHAGTLGRSFSLLRV
                     SNPRVRVLALKKAESGDELIVRLVELDGRRQNNVHIGFAAKVIAAREVNGQEMPLGKA
                     RVVDGRLVSSFTPYQLRTFAVRLAAPAAKARLPHSMPVALPYDVVVSSRDGSRSAPGF
                     DAAGRAISAEMLPRDVDFAGIHFHLGTESAANAVVAHGQSIALPAGDHKRLYLLAAAD
                     GDQRTTFIVDGKPTELTVQNWTGYVGQWDNRLWTTKQETLPPRPDAPPNAPIRTRMAT
                     VFNGLTPGFIKRAPIAWFASHRHTADGANEPYAYSYLFAYAIDLPPGAHTLTLPDNDK
                     IRVLAATVSDEDSSLVEAQPLYDTLIRSADDADGRR"
     gene            complement(9141..9785)
                     /locus_tag="VNN25_10885"
     CDS             complement(9141..9785)
                     /locus_tag="VNN25_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92076.1"
                     /translation="MKHFLAVALFAVAARAEVTVTVDHNRGAAATTAFKFARVASPAR
                     DDVAAAAKVEVIAGTVDRNSAPITALTDGRLPGNEDEPEANLFFRGDSWGGRIRFDLG
                     SIVDVAAVNTYSWHPDTRAPQVYRLYASDGTDPNFNPAPGVKVDLSTCGWREIAFVDT
                     RPKVGESGGQYGVSIADTTGSLGRYRYLLFDVFETESDDPWGQTFYSEIDVVKK"
     gene            complement(9978..11543)
                     /locus_tag="VNN25_10890"
     CDS             complement(9978..11543)
                     /locus_tag="VNN25_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92077.1"
                     /translation="MRSVILLVFLVAANAFAQNDAGEVSFANSGAPDAQAPFLRGLAL
                     LHDFEYESAAQAFRRAETIDPSFAMAYWGEAMTYTHPVWFQQEADSATAALKRLGATP
                     AERLAKAKTERERDYLRAVEILYGDGTKDERDFKYAEAMRVVHEHYPDDVDATAFYAL
                     ALLGTSHQGRDFATYMRAAALLEEVLPAHPQHPGVLHYLIHCYDDPIHAPLGLRAARR
                     YGLVAPGAGHALHMTSHIFIAMGMWDDVIDANRRAIDVVNKQRAARSKPSVACGHYPL
                     WLHYAYLQRGRFDDARTALDACRQSAFDPKFESAGPMDSAAGRIESFAEMRAHQIASG
                     GALTVADNVAIPDGLEFANARFLVAYGDVLVSAAHGDTAALKSAVARLHVLQQAAVQS
                     IEHQHSMNAMNPTFHIRAEVMVQQADALQLAAEGKRAEAIAMLQKAAAAETSMPFEFG
                     PPVVEKPTYELLGDELLAAGRASEAAAAYRSALERTPGRTASVEGLAKAQKTGDGSKM
                     LSELRVLATAPSS"
     gene            11695..12522
                     /locus_tag="VNN25_10895"
     CDS             11695..12522
                     /locus_tag="VNN25_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH92078.1"
                     /translation="MVLGIAQDGGYPQAGCNRPDCAAAWRNPKLRQRIASLAIVDPQS
                     HQRWIIDATPDFGSQLRTLDEVAPRSDASPLLDGILLTHAHIGHYLGLAELGREVIGA
                     HSVRVYAMPRMREFLTHNGPWDQLVTLHNIEITPIDDGVEVALNERIHVTPIRVPHRD
                     EYSETVGFVVRGPSRSILWLPDIDKWERWSTPLESILARVDVAYIDGTFYDASELPGR
                     DLREIPHPLMTETLSRLATSPMLAKVRFIHLNQSNPLLRGSRPGVVVAKDGDRTGLN"
     gene            complement(12878..>13359)
                     /locus_tag="VNN25_10900"
     CDS             complement(12878..>13359)
                     /locus_tag="VNN25_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXH92079.1"
                     /translation="RVLGTASTKHLHIATEAGCDVVIDYTKDDFEAVVMSETDGKGCN
                     AVYDSVGKMTFDKSLNCCGLRATLGLFGQSSGVVPPFDPSRLAKNGVFLTRPSLGHYI
                     HTREELLDRARELFEWIGSGRLKLRIDREVPLRDAADAHRALEARETTGKVLLIPGN"
BASE COUNT         2438 a         4322 c         4236 g         2363 t
ORIGIN      
        1 gccgagggat accaacgacc ctccgagcgc ttctggtcga ggaagagccg gcggaccacg
       61 atggccacgt agctggcaaa ggatgagcgt ccctcgaact tgcgaaggac tgcgcagtcg
      121 tcggcgagca gtgcgatccg cgcagacgag gcgaagtcct ccgcgtccgc gccgcgcagg
      181 cggacctgac gcgcaacctg cgtgatcgca cgctcgatca catcgaggtt cgcctcgaac
      241 aactgcggag gatgcatggg tggaatccca tcatcataat gccagaattg ctgaagagat
      301 gcgctcatgg cggaccacag ggcggacgcg aagcctgcat cccgtcagga cggcgatggg
      361 ggagcggttt tccagggatc gaggtgaggt ctttcgaaag agatcaaaga cggtcacgcg
      421 aagacacgaa gacgcgaagg acgccgaagg acacaggaca cagaggacgc ggagactgct
      481 acgtcgcgac tcgccgtccc cgcgcggcga tcgcataaag cgccgccccg atcgcgcccc
      541 agaaaaccac cagctcgatc gtcgtcagcg aggcgtgctc gatgcgctcc cggaatgaga
      601 ggtgcgtcaa tacgtcgacg tcgccgcgaa gctgcacggc gatcagcgtg cccatgaaca
      661 gcaacgagat cgtggcgctg atctgctgca cccaacgcgg aaggccgagg gtcacgctgg
      721 cgtacagcga gggatttgcg cggggcagtg ccagcagcgc gaggctatgc aggaagtaga
      781 ggcagaccag cgcgaagacg gcgatgttca gcgcgagcga gacttgtccg ctgacgagga
      841 ggagcagcgt cacgatcagt gtcaaggtca agccgacgat cggtgtgccg gtacgctttg
      901 aaatcgcccc gatgacctgc ggcgcgagtc catcctcggc cagcatgatt ccgacgcgcg
      961 atggaacgag catggttgcg ttgaccgagg tcgtcagggc catcaccgcg ccgagcgtga
     1021 cgaaccatgc cgcgcccatc ggcagatagc gcgacgccac cgcggccatc ggcgcggcgg
     1081 cttgtcgcag ctcggcgcca ggaaggacgc cgaatgcgac cacggacatc agaaagtaga
     1141 tgaccgtcgt cgcgagtatg ccgcgaagga agatgcgcgg cagccgacgt gtggagtcgc
     1201 gcacctcgcc ggccgtctgc gccagcgact cgaagccggc gtagccgaag aaaaccaaag
     1261 ggagcgctgc tgcaaatccg ccgagaccgt gcgtgaagaa cggctggtag ttgtgcgaat
     1321 ggatggcgaa gaggcccggc ccgaccagga cgaggatcga cagaccaagg agcacgcaca
     1381 tccagacctg gatgcgaccg aaccagcgca cgccggcgac gtggatgacg taaaagaata
     1441 ggaggctgcc gatcgccacc ggcatggcgg cgctcttcgg caacgcgccg gcgaagagct
     1501 cgaccgtgta gtcgccgagc gcgcgcgaca ggtaggccag cgcgccgatg tacgagatca
     1561 ctttgagcca cactccgacg aacgcgagac cgtagccgcc gaacgtgcgc gacagatgcg
     1621 tgtactcgcc gccgggctcg cggcctagcg gcgtggagag gaagaccgaa tacgcgaccg
     1681 acgtcgccag gatcgctggc gcgagcacga ggtagccgag aatcacgctc ggcccggtcc
     1741 gcgcccaggc atcgctcgtg actttgaaaa tgccggcgcc gatgagcatg cccagcagcg
     1801 tggcgtacga ctcgatggtg ccgaggtcgc ggcggagtga ggcggagagg ggtgcggcca
     1861 tgattcagtt cctttcggcc gcgcgcgaag cacgccagcg gtccgcggcg accgcgagca
     1921 cgatcacgac gccgatgatg atctcctgca cgtagttcgg ccagcccatt tgctggctgc
     1981 cgttgcggag aaaggccatg accagcgccc cgatcatcgc gccggcgatg ctcccttccc
     2041 cgccgctgag gctcgcgccg ccgatgacga cggcagcgat gacgtcgagc tcgacgccga
     2101 cggcgactgt cgggtcgccc tgacgcaaac gcgacatctg catcacgccg gcgaggccga
     2161 agaacaatcc cgccagcgcg tagatgcgca ctttcagcgc gcttgtgttg atgccgcagg
     2221 cgcgcgccgc cgcttcgttc gagccgagcg cgaacacgtg gcggccgaag acggtgttgc
     2281 gcagcacgag cgctatcatc gccgcgagga tgatcgcgat ccagacgccg ggcgcgaaga
     2341 gcatccagtc gcgcgatgga aaggtcaccg ccagttcgtt gagccacgtc gcgggcgggt
     2401 tgacggtctg ttccccggcg acgtacttgg cgacgccgcg cgcgataccg agcatgccga
     2461 gcgtggcgat gaatgggacg acgcgcagcg cggtgatagc cagtccgttg acgatgccga
     2521 cgatgccacc ggcgatgatg ccagcgagca acgcggcgat cggcggccag ccatcgtgca
     2581 aggccaacgc cgtgacgacg ctcgtgagcg cgatgacggc gccgacggag aggtcgatgc
     2641 cgccgctgac gatgatcatc gtcatgccga gggcaccgat ggcgacgatg acggtttgcg
     2701 cgagcacgac gcgcaggttg gcgcccgaaa ggtaacgcgc cggcgatccg gtcagaatcg
     2761 caaagagaag gatgaccgca acgaggccgg cgagcggagc gaggacgcgg agccagcgtt
     2821 tcatgcgagg tgcggattgt atgcggggga ccgtgttcgc ggtacgtagg tcggactctc
     2881 cagtccgact gcttctgacg aacgacggtt ccggcgcaaa cgcgaagtcg gactggagag
     2941 tccgacctac gtttcccgac ctacgtttcc gctccgatcg ctgcctgcag aatgctgtcg
     3001 gcggtccatt cgctggcttt gcagccggcg agcggagcga ggacgcggag ccagcgtttc
     3061 atgcgaggtg gggattgtat gcgggggacc gtgttcgcgg tacgtagatc ggactctcca
     3121 gtccgactgc ttccgacgaa cgacggttcc ggcggaaacg cgaagtcgga ctggagagtc
     3181 cgacctacgt ttcccgacct acatttccgc tccgatcgcg gcttgcagga tggtgtcagc
     3241 agtccattcg ctggcaggct ttgcagccga cagacggccg cgcgtcatca ccgcgatgga
     3301 gtcgcacacc gacagcagct ccggcagata cgagctcacc atcacgatcc ccttcccctc
     3361 ggacgccaag cgcgcgatct cgcgatagat gtcggccttg ctgccgatgt caatgccacg
     3421 cgccggctcg tcgagaagga gggtgtcggg atcctggtga agcagccgcg ccagcgcgac
     3481 cttctgctgg ttgccgccgg acagctttcc gacgataccg cgcggccccg gcgccttgac
     3541 gcgaagacgc gtcacgacat cgtccgtctg tgcgttctgc tggcgacgat cgatccatcc
     3601 gccgcgcgag caggtcgcga aacggctcat tgtgacgttg tcggcgatcg gcagttgcag
     3661 cgcgagccct tcgcctttgc ggtcttcgct gaggtagccg aaaccgttgc ggatctgatg
     3721 ggcgagtgtt ttcagctctt tccctcgcaa ccggacgata cctccggttg cccgctgaag
     3781 gccgaagagc gcgcgaacga gatcgctgcg gccggagccg acgagaccgg cgatgccgag
     3841 gatttcgccg cgatggagga gaaagcttgc cacgcggagg cgcggagggg cggagaggtt
     3901 ctcgacttcg aggagaactt cgggctgcaa atttgctcgt ggctcatgcg cagcttgatg
     3961 tggcacagac actcttgtct gtgctcccac catcatcgag atcagttcct catcgcgcac
     4021 ctcggtcagt tcaccgctgc cgatactccg gccatcgcgc agaaccgtga accggctggc
     4081 aatctcgcgc acctcttcca ggaaatggct gatgtagatg atggcgattc cgcgatcgcg
     4141 gaggcggcgc acggtctcga agagccgctc gacatccgct cgctgcaggc tgctcgtcgg
     4201 ctcatccatc agcagaatgc gggcatcgcg cgccagggcg cggcagatct cgacgacttg
     4261 ccggtccgcg agcgagagac tggcgacacg cattgaaggc ttgatctcgc tccgtccgaa
     4321 ctcggcgagc agcgtggccg cgcgctcgtt catcaggtcg cgacgcagcc atccgccacg
     4381 cgaaggctcg acgcccatcg cgatgttttc ggcgacggag agatgcccac acaacgccag
     4441 ctcctgatga atatgcgcca ctcctcggcc acgcgcatcg gccggcccgc gcggggcgta
     4501 cggcatccca tcgacgatca tcgagccgcc gtccggcgcc accgcgcccg acagaacatt
     4561 catcagcgtg ctcttccccg ctccgttctc gccgatgagc gcgtgaactt cgccgggcgc
     4621 gacctcgaga tcgacgccgt cgagggcgat cgtggcgccg aagtgtttgg tgatcgcatg
     4681 taaccgcagc gcgggcgagg atgcacgcga gacgcctgcg cttgtcgtgc gcatcacttc
     4741 aaatacgtct ccagcggcgg atgcagcaac gtctgcgatt cctgcgcgtc gaggttcgtg
     4801 ggggtgatca gcgtgactcc ggtatcgacg cgtttctcga cggcgttgcc gaggaggtga
     4861 tcgacgacgg ttttgacgcc gagctcgccc atacgaaacg gattctgcac cacgatgccc
     4921 tgcagcttgc cgtcgcgcat cgctttcacg aagacctcgc tcgtgtcgaa gccgatgaaa
     4981 cgaatcttgc ccgctttgcc ggcgtcttcc agcgccagca acgcaccggc agtggacgat
     5041 tcgttcggcg tgaagagacc atcgaggtcg ttgccgaatc gattgagcag attctcggcg
     5101 gccgtcttcg cggtgtcgcg cgtcgcgccg gcgaattgat tcgaggagat gagctgaatg
     5161 ttcggatagg cagctttgat ccgctcgatg aaaccctttt cccgctgctc ggtgctgaag
     5221 acgccctcct gatagcgcag cgcaaggact ttgcctttgc cgccgaggag gcggcccatc
     5281 tcgtcggcgg caagctcgcc gccgtggtag ttgttactgg ccacgaagct gatcggatcg
     5341 gttgattcca ggccggagtc gacaacgact gtggggatgc cagcgcgctt cgcttcctcg
     5401 acgggacgaa cgagtgcgtt cttgtcgaag ggagcgagga cgatgccgtc gacatgctgc
     5461 gtgacgaagc cttcaacgac ctgcacctgc tgctcgcggt cgtcctcgcg catcggaccc
     5521 ttccagatga tggtgacgtt gacgcccgcc ttcccctcgt cctgcgccgt cttcatcgcg
     5581 ccggcatgaa tgctcttcca gaactcatgc gtggagccct gcgggatgac ggcgacggtg
     5641 aaggacttgg cggattttgc tgcggtagtc tgctcgtgct ttgcacagcc gatgacaacc
     5701 attgcgatcc cggcagcgat catgattcgt ttcattcggt cagctcctga caaagcggcg
     5761 tgtggttttt catctgcgtc catctgcgtc atctgcggat cggatcaatg tgtcatacag
     5821 cggttgcgcc tcgacaagcg atgagtcctc atccgaaacc gtcgccgcca gcacgcggat
     5881 cttgtcgttg tcgggcagcg tcagcgtgtg cgcaccggga gggagatcga tggcgtacgc
     5941 gaacagatat gagtacgcgt agggctcatt cgcaccatca gcagtatgcc gatgcgaagc
     6001 aaaccacgcg attggggcgc gtttgatgaa gccgggcgtg aggccgttga acacggtcgc
     6061 catgcgcgta cgaatcggcg cgttgggcgg cgcgtcgggc cgcggtggca gtgtctcctg
     6121 cttcgtcgtc cagaggcggt tgtcccactg gccgacgtag ccggtccaat tctggaccgt
     6181 gagctcagtg ggcttaccat cgacgatgaa cgtggtccgc tgatcgccgt cggcggcggc
     6241 gaggaggtag agccgtttgt gatcgccagc aggaagtgcg atcgactggc catgcgcgac
     6301 gaccgcgttt gcggcgctct ccgtgccgag gtgaaagtga atgccagcga agtcgacgtc
     6361 gcgcggcagc atctcggcgg aaatggcgcg accagcggca tcgaagcccg gcgccgatct
     6421 cgaaccgtcg cggctcgaga ccacgacgtc atacggaaga gcgaccggca tcgagtgcgg
     6481 caggcgagcc ttcgccgccg gtgcggctag gcgcactgcg aacgtgcgca gttgataggg
     6541 cgtgaacgaa gacacgagcc gcccatcgac gacgcgcgcc ttcccgagcg gcatttcctg
     6601 tccgttcact tcgcgcgcag cgatgacctt cgcggcgaag ccgatatgga cattgttctg
     6661 ccgcctgccg tcgagctcca ccaaccgtac gatcagctca tcgccgctct ccgccttttt
     6721 caacgccaga acgcgaacgc gtggattgct aacgcgcaac aatgagaacg accgccccaa
     6781 cgtgccggcg tgcttcggcg acacgaacgc gatcagcggc tgactgagcc ggagcgcctg
     6841 ccagtcggtt ccttccgatc gccagtctcc ggcatgcacg gcaagcccgt aaacaaactc
     6901 gtgatgcccc caatcctgcg aggcctgatc gctgtaggcg cggccgttcg cgctgaggcc
     6961 cggcgtgtaa agcagcgtga gccgcagcgt gtgatcgtcg ggtttgtcgg atccgtattt
     7021 gctgtcggta aggacggtta cgccgccctt tgcatcgccg agatcgaacc actgatgcga
     7081 cgccacctcg aactttttct cgtcgttcgt ggcacgctcg atcgtgccca cttcccagtt
     7141 gtaggtggcc agcttgttgg acgcggtcag cggaaacgtc gccttcagcg cggccgcctc
     7201 cgttttccag tcgatggcgt tgtcgatctc gatgcgattg ccgccatcgc cagtagcgag
     7261 gcggatcgtc tgcacgaatc gcgagccttc cgtctctcgc gtcacttcca gcgccgcgcg
     7321 cgccggcccg ttttcgacga tgcgcatcgt cgccggtcca ctgacgtagc cgcgcggcgg
     7381 cttctgctga tcggcccaat ccatgttcca ggcgggccag tcgtgcggat tctcagtctg
     7441 aagtgcgagg cgcgctggtg cagacaggac ttcgcgcttc gtcttcttgt cgaagatact
     7501 ggcgaggtcg cctgcctcgt cgatcgaaac gcggtaccgc gcgttgtcga gggtagcgcg
     7561 cgcttgccgg gagtcgggag tcgggggtcg gagctgcgcg cgaagtgccg attctcttcc
     7621 ggctccgaca cccgaccccc gactcccgac tcccgcgtca cgaacgtcgt acaccgccca
     7681 tcccaccgac ggcaccttag ccacgaagat caccgtgtcg ccgtcgcgct gcgaccgaac
     7741 cgccctgccg tccggcccaa acacgctgac gtcctcattc tccggaagcg acacgtgcgc
     7801 ctcgaccaca tcctcccgcg cgatgctcag cgggttgtaa acgacgattg ccgttccctt
     7861 gacgcgcgtg tcgagcgccg acgccaccgc ttccgtcgcg ctcgtcacca cgcccgcgaa
     7921 ctggttcatg gcgatgacgt cgtcgttcca cgaatactcg taagccttcg gcgtggcggt
     7981 gccggcggcg atgtcgtgga actggccgcc catcaccagc gtccatgcgt cgttcaatcg
     8041 tttcatcgga taggcacgcc cgccgagcca ttctgcggcg accgaggcgc gttcggcggc
     8101 gtcggccagc agctcgttct tgtgattcca ccgcttctga tacgcctcgg acgtcagcga
     8161 cccggcggag tgattcgtca gctcgagctc gcccttgtac cgtggaagtc cggcccgctg
     8221 cgacggtttg atatcgagaa acatctcctc ggcagtcgag gagatcacgt gcaaaggacc
     8281 gtcgttcttc gacacgatcg actcgaccag ttgcaccgag ggctcgcggg gcgcgccgcc
     8341 gacatcgccg gttccgtagt agtgatagtc ggtgaagacg ccgctggccg cgccgttgcg
     8401 ctccacccgc tttggccagt cgactccgtt cggcggcgtc gtgtcgctgc gcgagaggtc
     8461 gtagcgaatg tccgcgctgt agctgccggg attgaacgcg gcaaggacac tgttgccgtc
     8521 gggaccctcc cacactccca cgttgaacgg cgtccccacc ggcgttttct ccggcgagtc
     8581 cgggccgccc accggcgccg acgatcccca cgtcagcttc tgcgtagaga atccaacgat
     8641 ccccatgtga tgcaggatgc tcggcagcga cgctggaaat ccgaagcagt cgggcagcat
     8701 gtactccgcg ctggtgcgcc ccagctcgcg ccggaaatac gcgttgccgt agaggacctg
     8761 gcgaatgatc gactccgccg acggcgagtt tacgtcgctc tcttccatcg acgatccggc
     8821 cgggaaccac cggcccttgc gcacccattc ctgcagcacc gcgtaatcgg ccgggtagta
     8881 ctcgcgcatc atccggtaac gattggcgcc gctgaagttg aagatgtagt ggggatattt
     8941 ttcgaagagc gcgaagttgt cgtgcatcgt gcgcggcaga aattctttga tggtctgcgg
     9001 atactcccac cgccactgcg tgtcgagatg ggcatacccg acgacgtaga gcgttggctg
     9061 cttcgtgagg tcgggaagat cggccgcttg cgcaactgcg gtacacgcga agagcaacgc
     9121 gatcatcgcg cccagcttct tcatttcttc accacatcga tctcgctgta gaacgtctgc
     9181 ccccacgggt cgtcgctctc ggtctcgaag acatcgaaga ggaggtagcg ataacggcca
     9241 agcgagcccg tcgtatccgc gatgctcacg ccgtactgcc cgccgctctc ccctaccttc
     9301 ggccgcgtgt cgacgaaggc aatctcacgc cagccgcagg tggacagatc gaccttcacg
     9361 ccgggagcag gattgaaatt cggatcggtg ccgtcgctcg catacaaccg gtagacctgc
     9421 ggcgcgcgcg tatcgggatg ccacgagtac gtattcacgg ccgcaacgtc gacaatcgat
     9481 ccaagatcga aacgaatacg cccgccccac gaatcgccac gaaaaaagag attcgcttcc
     9541 ggctcgtctt cgttaccggg aaggcgccca tcagtcagcg ccgtgatcgg cgcactgtta
     9601 cgatcgactg tcccggcgat gacttccacc ttcgcagccg ccgccacatc gtcgcgcgcc
     9661 ggcgaggcca cccgcgcaaa cttgaacgcc gtagtcgctg ccgccccccg gttgtgatcc
     9721 accgtcaccg tcacctcggc acgcgcagca accgcgaaga gtgcgaccgc aagaaaatgt
     9781 ttcacgacgg gaatggtaat tcgatttcac gcgaagacgc tcacgcgaag acgcgaaggc
     9841 gcgaagccat aaaaagagat ggagcgaacc ttcgcgcctt cgcgtcttcg cgtgagcgtc
     9901 ttcgcgtgag cgtcttcgca cggaatcgcc ttcgcatgag ccgtcccttc cccgcgaccg
     9961 cggggttact ggttcactca cgaggatggc gcggtcgcga gaacccgaag ttcggaaagc
    10021 atcttcgatc catcgcccgt cttctgcgcc ttcgctagcc cttccacact cgccgtcctt
    10081 cccggcgttc gctccagcgc cgagcgatac gccgcagcgg cctcggaggc gcgtcctgcg
    10141 gccaacaact cgtcgccgag gagctcgtag gtcggcttct cgacgacggg cggcccgaac
    10201 tcgaacggca tcgacgtctc cgccgcggcc gctttctgca gcatcgcgat cgcctcggcg
    10261 cgcttcccct ctgccgcgag ctgcagcgcg tccgcctgct gcaccatcac ctcggcgcgg
    10321 atgtggaagg tcggattcat cgcgttcatc gaatgctgat gctcgatgct ttgcaccgct
    10381 gcctgctgca gcacgtgcag ccgggccacc gcggatttca acgcggccgt atcgccgtgc
    10441 gccgcggaca ccaacacatc gccgtaagca acgaggaagc gggcgttcgc gaactccaag
    10501 ccgtcaggaa ttgccacatt gtctgcgact gtcagcgcgc ctccgctggc aatctgatgc
    10561 gcacgcatct cggcgaacga ttcgatccgc ccggcggcgg aatccattgg ccccgcggat
    10621 tcgaacttcg gatcgaacgc cgattggcga caggcatcga gagccgtgcg cgcatcgtcg
    10681 aaacgccccc tctgcaggta ggcgtagtga agccagagcg gatagtggcc acacgcgacg
    10741 gacggcttcg agcgtgcggc gcgctgcttg ttgacgacgt cgatggcgcg gcggttggcg
    10801 tcgatcacat cgtcccacat gcccatggcg atgaaaatgt gcgacgtcat atgcagcgcg
    10861 tggccggctc cgggcgcgac gaggccgtac cggcgcgcag cgcgcaatcc gaggggcgcg
    10921 tggatcggat cgtcgtagca gtggatgagg tagtgcagca cgccgggatg ctgcggatgc
    10981 gccggcagga cctcttccag caacgccgcc gcgcgcatat aggtggcgaa gtcgcggccc
    11041 tgatgggatg tgccgaggag ggcgagggcg tagaacgcgg tagcgtcgac gtcgtccgga
    11101 tagtgctcgt gtaccacacg catggcctcg gcgtacttga aatcgcgctc gtccttcgtg
    11161 ccgtcgccgt agaggatctc gacggcgcga aggtagtcgc gctcgcgctc cgtcttcgct
    11221 ttggccagac gctcggcggg cgtcgcaccg aggcgtttca gcgcggccgt ggcggaatcg
    11281 gcttcctgct gaaaccagac cggatgcgtg tacgtcatcg cctcgcccca gtacgccatc
    11341 gcgaacgaag gatcgatcgt ttccgcacgg cgaaatgcct gcgccgccga ttcgtactcg
    11401 aagtcgtgca gcagcgcgag gccgcgcaga aacggcgcct gcgcgtcggg cgcgccggag
    11461 tttgcgaacg acacttcacc ggcgtcgttc tgggcgaatg cgttggcggc gacgaggaag
    11521 acgaggagaa tgacgctgcg catgactgga atgatagaac ctgttgatcc gcagatggcg
    11581 cagatgagcg cagatatctg tgtcaatctg cgtcatcttt taacaaaggt tttgcttctc
    11641 attctgttca ccgcctgcgc aaccgtccct ccgcccgcga caacaccctt caccatggtc
    11701 ctcggtatcg cgcaggatgg cggatatccg caggctggat gcaatcgtcc agactgcgcc
    11761 gcggcctggc gcaatccgaa attgcggcag cgtatcgcgt cgctggcgat cgtcgatccg
    11821 caatcgcacc agcgctggat catcgacgcg actcccgact tcggatcaca actgcgaacg
    11881 ctcgatgaag tcgcgccgcg gtcggacgcc tctccccttc tcgacggcat cctccttacc
    11941 cacgcccaca tcggccacta cctcgggctc gccgaactcg gccgcgaagt catcggcgcg
    12001 cattcggtgc gcgtctatgc gatgccgcgt atgcgcgaat tcctcacgca caacggcccc
    12061 tgggatcagc ttgtgacgct gcacaacatc gagatcacgc cgatcgatga tggcgtcgag
    12121 gtggcgctga acgagcggat ccacgtcacg ccgatccgtg taccgcatcg cgatgagtac
    12181 tccgagacag tcgggttcgt cgtgcgcggc ccctcgcgct caatcctgtg gctgccggac
    12241 atcgataaat gggagcgatg gtcgacgccg ctcgagtcga tcctcgcccg cgtcgacgtc
    12301 gcctacatcg acggcacgtt ctacgacgcc agcgagttgc cgggacgcga tctgcgcgag
    12361 attccgcatc cgctgatgac ggagacgctg tcacgccttg caacgtcgcc gatgcttgca
    12421 aaagtgcggt tcattcatct gaaccaatcg aacccgctgc ttcgcgggag caggccgggc
    12481 gtcgtcgtcg ctaaggatgg ggatcggact gggctcaatt gacaattgat aatggacaat
    12541 tgacaacgtg gtttcgcgtt cgtcgccaac gcgtttgcct cgtctctcgt ccgtgaggcg
    12601 acgacccgac cggcggtgtc atcctgagcc tgccggaggc gcccgcacga ctcgcgcctt
    12661 cacgcaggat gggacggcgc cgcaggacgg cgaaggatct caggttgccg ggcacatggc
    12721 ttgtgcgtcg taatttgaga tccttcgacg tcctacgccg ccacatcttg cagcgcgttc
    12781 ggctcaagta cgaatggcgg ctccggcagt ctcaggatga caccttggat aacaccttcc
    12841 atcgctaatc ccgttgtcaa ttatccattg tcaattgtca attgccagga atcaacaaca
    12901 ctttccccgt cgtctcccgc gcttccaacg cacgatgggc gtccgccgca tcgcgcagcg
    12961 gcacctcgcg atcgatgcgc agtttcagcc gtcccgagcc gatccattcg aacagctcgc
    13021 gcgcacggtc gagcaactcc tcgcgcgtgt ggatgtagtg accaagactc ggccgcgtca
    13081 ggaacacacc gttcttcgcc agacggctcg gatcgaacgg cggcaccact ccgctcgatt
    13141 gcccgaagag cccgagcgtc gcgcgcagac cacagcaatt cagactcttg tcgaacgtca
    13201 tcttgcctac cgaatcgtat acggcgttac atcccttgcc gtccgtctcc gacatcacga
    13261 ccgcttcaaa atcatccttc gtgtaatcga tcacgacatc gcaccccgcc tcggtcgcaa
    13321 tatgcagatg cttcgtcgac gccgtcccca gaacgcgcg
//
LOCUS       DATLVW010000359         9291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_304691, whole genome shotgun sequence.
ACCESSION   DATLVW010000359 DATLVW010000000
VERSION     DATLVW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304691"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VNN25_10905"
     CDS             <1..469
                     /locus_tag="VNN25_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH92080.1"
                     /translation="VGMRVSRLAAAFGVRVTAFDPYITDDDFAQRGAMRANTLDTLLR
                     ESDIVTLHVPFTAETRNMIGANEIALMPRGAIVINAARGEVLDVDAALAALARNDLGG
                     LALDVYDPEPPTRTWPDDPRLILTPHIAGCTTEAKSAIGAKLYEKICAHYAAR"
     gene            491..877
                     /locus_tag="VNN25_10910"
     CDS             491..877
                     /locus_tag="VNN25_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92081.1"
                     /translation="MFCPECQSEYRDGITHCSDCDVDLISDLSEEVPQHLGNLLPLAE
                     ERSPELVAELIDRLEKADIPYVIEAGTALAMLTSEEIVFDKPEPWEARVWIPASLGER
                     GTSILAEVDEQFGRRRAGIRQAPQPL"
     gene            complement(874..2175)
                     /gene="hutI"
                     /locus_tag="VNN25_10915"
     CDS             complement(874..2175)
                     /gene="hutI"
                     /locus_tag="VNN25_10915"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXH92082.1"
                     /translation="MKLSPQIPHPPLPTPHSPTTLIRNLSQIATPLGFVPARGESMRN
                     VAVFDDAVIVIRDRRFAFVGHESDIDPDLRGVIDEDFDARGATAVPGFVDSHTHIPFA
                     GFRETEFNRRLQGETYENIAASGGGIASSVHATRRASQDELTGNILARTRTMARYGTT
                     TAEAKSGYGLSVEGELKQLRAIRDASPSSLVRLVPTCLAAHDFPPEGRSSAAARRGYV
                     SMIIGEILPAVADERLAVFCDAFVERGVFTIEEGDAVLRAGAALGLFPRLHADELSDT
                     DGASLSAALGCASADHLMHVSDAGIDALAASNTVANLLPATSFFLMSDRYAPARKLID
                     ADAIVSLSTDCNPGTSMTESMPMVMQLATLQMKMTVEESLTAATLNGASSLRLAHEIG
                     SIEAGKRADLVLLDAPNYLHLVYHFGVNLVTRVMRDGAWVV"
     gene            complement(2269..2766)
                     /locus_tag="VNN25_10920"
     CDS             complement(2269..2766)
                     /locus_tag="VNN25_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXH92083.1"
                     /translation="MQGLTPLDIQKQTFARVFKGYNVDEVRGYLHLIAEEIEGLIRDV
                     DRLSRENSMLRDELDEHNARERILKDTLLSAQKVSEDLKANAHKEAELIVKDAELLSD
                     RLTSQAMARVGDLERAIQDLKIERRGNRNKLQSMLDTMQQLVMLDAEQEANEQPITAM
                     FRSRE"
     gene            2870..4447
                     /locus_tag="VNN25_10925"
     CDS             2870..4447
                     /locus_tag="VNN25_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92084.1"
                     /translation="MNKLMALTLFSLLATVSAWAAAPDNDASCDIKVGPAATLLLPYF
                     EVDVGFNPQPTDQTTLFTITNVSRYSQIAHVTLWTDWAFPVLNFNIFLAGYDVQSINL
                     KDILVRGVVASPVGTGPTTVKSPLGTLTSPANGGPGYANPNFNAVIECDALPGILSQT
                     LVAAVRSALTTGVYNPGGSAACPNAVGGVHANAIGYATIDVVGSCTSRLPSDPLYYTT
                     DLLFDNVLIGDYQQIGPKPAGRSNESFDAAGNPMVHIRAIPEGGAAGSNVATELPYTF
                     YDRYTPDTNRILDRRQPLPSTFAARYIQGGFADFNTNYTIWREGFGKGACGASLVLNA
                     QMPVAEIVRFDETDNPSVGPPCNAEPCPLPTLPTLAVTSSTRTSSGLYPTMSIDGVGG
                     WMYLNLNNGGSTGYSVTTRSVFGDVPTVITGAGSRSALAPSGSATIGPRPSQNWVTIT
                     MFGTLGTKRMVAEFDAASLGNGCSPAAVSTATFLFPTGNYGPGTIGPAVFVCPPGATL
                     SNGTTNLCTGTNINPKP"
     gene            complement(4462..5508)
                     /locus_tag="VNN25_10930"
     CDS             complement(4462..5508)
                     /locus_tag="VNN25_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92085.1"
                     /translation="MKKHRSIHPGVLPDYSIPVAGMLLALCVMTSVAPNAKASAPAAP
                     KHATTTPGNPTAVADNGTVTGTLTLNRKAITLRHIYGRKREAWPMDTKPFNVDSVDEL
                     SCGIIEVMITNLPLSEAVLTSILQNEYHGSDKIRGICLTFEASGKHSFVHQFLLESGA
                     AEGFGITQTDGEMKMDHGRVIGSVKCKNDENTDTRVFKVSFDTGVKLQYSPPEGAERI
                     APDQFPDEYLRVVPGKWNVERWVGLGCFKASGTLSVVERSGPHEFRGAFHLVVSQPDH
                     PDGIVDEDIIITRDGTRVHFEGSNLRAGDVAWTRDVLNFELRKGLLLGGGVTDSVVLK
                     KVQESQETTKPAER"
     gene            complement(5545..5877)
                     /locus_tag="VNN25_10935"
     CDS             complement(5545..5877)
                     /locus_tag="VNN25_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXH92086.1"
                     /translation="MGILVVFYDALMIVLQVIQWIVLVWVILSWVLFFASQTSFRWRY
                     KSAYVILNQLNDIFTRMTSPFLRPFRRLVPPHKMGGIDWSPLLLLLAIYILRGVLDYL
                     FRPLLLRG"
     gene            complement(5878..6537)
                     /locus_tag="VNN25_10940"
     CDS             complement(5878..6537)
                     /locus_tag="VNN25_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXH92087.1"
                     /translation="MSIRDNIAAVEERIARACVRAGRAREDVKLVAVSKTFPAEFVDE
                     AIAAGITDIGENRVQEARDKKPLVHGSVRWHLIGHLQTNKAKDAVKLFDVIEAVDSLD
                     LAEKLAHAAEGQGKTIDVMLQVNIGDEPQKSGLGREDVDVIAKQVGGLAPLRLIGLMA
                     IPPVGTPDESRPYFRELRSMRDALGLKELSMGMSEDFEAAIEEGSTMVRVGRAIFGSR
                     G"
     gene            complement(6524..7081)
                     /locus_tag="VNN25_10945"
     CDS             complement(6524..7081)
                     /locus_tag="VNN25_10945"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXH92088.1"
                     /translation="MQRLPHACPPGRAATAQARRSRSLRRLQPYPLSGESPILKIIAF
                     VDGACRGNPGPASYGVYMRKDDSEIIEISGFLGTTTNNVAEYAGLLEALSTAQMEGAT
                     DVEIVSDSLLLVQQMLGKYRVKHPNLVPLYERAKSITRRFPKFKIRHTLRAGNKDADR
                     LANAALDRPERTPNDRWIERRRVDS"
     gene            complement(6964..7674)
                     /locus_tag="VNN25_10950"
     CDS             complement(6964..7674)
                     /locus_tag="VNN25_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HXH92089.1"
                     /translation="MNPDVEKLWELQTVLSALGDREKQLTNKPESFAAVDREWQSVNA
                     EMEKLQQQIEQHSRERRRVDGELSDQQELLKKYQGQLMQVKNQQQYAAAWKEIDATRK
                     QVKELEDSTLKTMADVDGLQKQNDDLAAGSVELKSRWDAEHEAWQHSLGDLRKEVGQL
                     QARAAKIEASLPERLSREFHRIFKQRQGVAIARVVADSCNGCRTRVRPAVLQQLKRGD
                     LVHCEGCSRILYLESPPS"
     gene            complement(7658..7834)
                     /locus_tag="VNN25_10955"
     CDS             complement(7658..7834)
                     /locus_tag="VNN25_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92090.1"
                     /translation="MTNDRLHYRRGPAGGSLTLSAPALLTMLERPAGQFLTAQAFHHR
                     NAFFVFGGLVESGR"
     gene            complement(7861..7936)
                     /locus_tag="VNN25_10960"
     tRNA            complement(7861..7936)
                     /locus_tag="VNN25_10960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7901..7903),aa:Ile,seq:cat)
     gene            complement(8043..>9291)
                     /locus_tag="VNN25_10965"
     CDS             complement(8043..>9291)
                     /locus_tag="VNN25_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXH92091.1"
                     /translation="RPRQPWEGKMVSVRRVVPVLLLAVAIPIFGAPKKSSSHPPRDLH
                     RVGDHWTAYNPPDPATWPAGSKTYTIKRGDTLWSLAEQFFKNAYMWPQLWEGNTWITD
                     AHWIYPGDVLLIDSETAQQISEAQGGGHDSTLPPPPALMPESAPSMSSDTTSGNAFPG
                     GEGYISAADAAGGSRNPVPLGTDADVYCYGYIGEDGESMPNTILAFEDAEVRYAPGAV
                     EQVISGAVGDLVILKGGTASGLVAGETYILVDVGRLISHPAKRTQNVGREYLYRGQVR
                     ILCADEHQARGLITQSCSEVPVGTHLKPLPQIPIPLARIPDMPAFCDNSSNKKNGYIV
                     GAQGGWDAALGEGLLVEVNLGQADQLQPGDFLTVYTEEVAPGQPRQVLGEVGILTTNT
                     HTSTGKIVAMRYSMKIGDHVEIR"
BASE COUNT         1714 a         2916 c         2836 g         1825 t
ORIGIN      
        1 ggtcgggatg cgcgtttcac gcctggccgc agcattcggc gtgcgcgtca ccgcgttcga
       61 tccctacatc acggacgacg atttcgcgca gcgcggcgcg atgcgtgcga acaccctcga
      121 tacgctgctg cgggaaagcg acatcgtcac gcttcacgtc ccgttcaccg ccgaaacgcg
      181 caacatgatc ggcgcgaacg agatcgcttt gatgccacgc ggcgcgatcg tgatcaatgc
      241 cgcgcgcggc gaggtgctcg acgtcgacgc cgcgctcgcc gccctcgccc gcaacgacct
      301 cggcggacta gcactcgacg tctacgaccc cgagccgccc acgcgcacat ggcccgacga
      361 tccccgcctc atcctcaccc cgcacatcgc cggctgcacg accgaggcaa aaagcgccat
      421 cggagcgaag ctgtacgaga agatctgcgc gcactacgcc gcgcggtgat tgtgagaaga
      481 tcgctcacca atgttctgtc ctgagtgcca gagcgaatac cgcgacggga tcacgcactg
      541 ttcggattgc gatgtcgacc tcatctccga tctttctgag gaagtacctc agcatcttgg
      601 caatctgttg ccgctggctg aggagcgatc gccggagctt gtggccgagc tgatcgaccg
      661 gctcgagaaa gcggacattc cctacgtcat cgaagcggga acggcgctgg cgatgttgac
      721 cagtgaggag atcgttttcg ataagcccga gccgtgggaa gcgcgcgttt ggatccctgc
      781 gtcacttgga gaacgcggaa caagcatcct ggccgaggtc gacgagcagt ttggccggcg
      841 acgtgcgggg attcgacagg caccgcagcc gctctaaacg acccacgccc catcgcgcat
      901 cacgcgcgtg accaggttca cgccgaagtg atagacgaga tggaggtagt tcggcgcatc
      961 cagcaacacg agatcagcgc gcttgcccgc ttcgatcgaa ccgatttcgt gcgcgagccg
     1021 cagcgagctg gcgccgttga gcgtggcagc ggtcagcgat tcttcgacgg tcatcttcat
     1081 ctgcagcgtg gccaactgca tcaccatcgg catcgactcg gtcatcgatg tgccgggatt
     1141 gcagtccgtg gagagggaga cgatggcatc ggcgtcgatc agtttgcgcg caggcgcgta
     1201 gcggtcggac atcagaaaga aacttgtcgc cggcaggagg ttggccacgg tgttcgatgc
     1261 cgccagcgcg tcgatgccgg cgtcggagac gtgcatcaga tggtcggccg aggcgcagcc
     1321 gagggcagcg gagagcgagg cgccatccgt gtcggagagc tcgtccgcgt gaagcctcgg
     1381 gaagaggcca agcgccgcgc cagcgcgcaa taccgcgtcg ccctcttcga tcgtaaagac
     1441 gccgcgctcg acgaacgcat cgcagaagac cgccagcctc tcgtcggcga ccgcaggcag
     1501 aatctctccg atgatcatcg acacatatcc gcgccgcgcc gcggcactcg atcgcccctc
     1561 gggcggaaag tcgtgcgcgg cgagacaggt gggtacaagc cgaacgagcg aggacggact
     1621 cgcgtcgcgg atggcccgca gttgcttgag ctccccttcc acgctcaatc cgtatccgct
     1681 cttcgcctcg gcggtcgtgg ttccgtagcg ggccatcgtg cgcgttcgcg cgaggatgtt
     1741 cccggtcagc tcgtcctgcg atgcgcgtcg tgtggcgtgc accgacgagg cgatgccgcc
     1801 tccgctcgcg gcgatgttct cgtacgtttc cccctgcaac cgccggttga actccgtctc
     1861 gcgaaaaccg gcgaagggaa tgtgcgtgtg cgaatcgacg aagccaggca cggccgttgc
     1921 gccgcgcgca tcgaagtcct catcgatgac gccgcgaaga tcgggatcga tatcggattc
     1981 atgtccgaca aacgcaaaac ggcggtcgcg aatgacgatg accgcgtcat caaacaccgc
     2041 gacgttgcgc atggactcgc cgcgcgcggg aacgaagccg aggggtgtgg cgatctgcga
     2101 gaggttacgg atgagggtgg tcggggagtg gggcgtgggg agtggggggt ggggaatctg
     2161 aggagacagt ttcatgacgc gggagtggct tgttgggtgt ttgggagctt agcaggcgac
     2221 acgtgcttca ttcgatctcc cacaccccac ccccccacgc cctactccct actcccttga
     2281 acggaacatc gccgtgatcg gctgttcgtt cgcttcctgt tcggcgtcga gcatcaccag
     2341 ttgctgcatc gtatcgagca tcgattgcag cttattgcga ttgccgcggc gctcgatctt
     2401 gagatcctga atggcgcgct ccaggtcgcc gacgcgcgcc atggcctgcg acgtcagccg
     2461 gtccgagagg agttcggcat ccttcacgat cagctccgcc tccttgtgcg cgttcgcctt
     2521 cagatcctcg gagacctttt gcgcggaaag cagcgtgtct ttgaggatgc gctcgcgggc
     2581 gttgtgctca tcgagctcgt cgcgcagcat ggagttctcg cgtgagaggc ggtccacgtc
     2641 gcggatcagc ccttcgatct cctcggcgat caaatgaaga tagccgcgca cctcatcgac
     2701 gttgtagccc ttgaagacgc gcgcgaacgt ctgcttctga atatcgagcg gtgtaagacc
     2761 ttgcatcgac ccctcccgtc acgtgactgc gtgagtatac gcgtccaaat aaagatagcg
     2821 ccatatggtg taacagcata tattgccttc attcgcgcgg agggctgcaa tgaacaaact
     2881 gatggcgttg accctgttct cccttctggc cacggtttca gcgtgggccg cagctcccga
     2941 caacgacgcc agctgcgaca tcaaggtcgg tcccgctgcg acgttgctcc ttccctattt
     3001 cgaagtcgat gtggggttca atcctcagcc aaccgatcag acgacgctct tcacgatcac
     3061 caacgtgtcg cgctattcgc agatcgcgca cgtgacgctc tggactgact gggccttccc
     3121 ggttctcaac ttcaacatct ttctcgccgg ttacgacgtt cagagcatca acctgaagga
     3181 catcctggtc cgcggcgttg tggcgtcgcc cgtcgggaca ggtccaacga cggtgaagtc
     3241 gccgctggga acgttgacct cgccagcaaa cggcggaccc ggttacgcca acccgaattt
     3301 caatgcggtc atcgagtgcg acgctcttcc cggcatcctc tcgcagacgc tcgttgccgc
     3361 ggtcagaagc gctctgacca ctggtgttta caatccgggc ggttctgccg catgcccaaa
     3421 cgccgtcggc ggagtgcacg ccaatgccat cggctatgca acgatcgacg tcgtcggcag
     3481 ctgcaccagc cgtttaccct cggatccgct ctactacacg accgatctcc tcttcgacaa
     3541 cgtcctgatc ggcgactatc agcagattgg cccgaagccg gcggggagat caaacgaatc
     3601 gtttgatgcc gctggcaatc cgatggttca tattcgcgcg atccccgagg gaggggcagc
     3661 gggatcgaat gtggcgacgg agctgccgta cacgttctac gaccgctaca cgccggacac
     3721 caaccgcatc ctggatcggc ggcagccatt gccctccacg ttcgcggccc gttacattca
     3781 gggaggcttt gcggatttca acaccaacta cacgatctgg cgggaggggt tcggcaaagg
     3841 cgcgtgcggc gcttctctgg tgttgaacgc gcagatgccc gtcgcggaga tcgtacgttt
     3901 cgacgaaacg gacaacccaa gtgtgggtcc accgtgcaac gctgagccgt gccctctccc
     3961 gacattaccc acactcgctg tcacgtcatc caccaggacg tccagtggtc tctatccaac
     4021 aatgagcatc gatggcgtcg gagggtggat gtatctgaat ctcaataacg gcggaagcac
     4081 cggctactca gtcactacgc gtagcgtttt tggagacgtt ccaacggtga tcaccggtgc
     4141 tggcagcagg agcgccctcg cgccttccgg ctcggcgaca atcggaccgc gtccgagcca
     4201 gaactgggta acgatcacga tgttcggcac cctcggcacg aagcgtatgg tcgccgagtt
     4261 cgacgccgca tcgctcggca acggatgctc gcccgccgcg gtttcgacgg caaccttctt
     4321 gttcccgacg ggaaactacg gccccggcac gatcggcccc gccgtcttcg tctgtccgcc
     4381 cggagcaacg ctgtcgaacg ggacaacgaa tctctgcacc gggacgaaca tcaatccgaa
     4441 gccgtgatcg gtcgatgaat ctcagcgttc ggccggcttc gttgtttcct gagactcctg
     4501 aaccttcttg agcacgacgc tgtcggtgac gccgccgccc agcagcagac ccttcctgag
     4561 ctcgaaattc aagacatccc tggtccacgc tacgtcgccc gctctcaggt tgcttccttc
     4621 gaaatgaacc ctcgttccat cacgcgtaat gatgatgtct tcatcaacga tcccgtcggg
     4681 gtggtctggt tggctgacaa cgagatggaa tgcgcctcga aactcgtgcg gaccgcttcg
     4741 ttcaaccacc gacagtgttc ccgaggcctt gaagcagccc aatcccaccc agcgttcaac
     4801 gttccacttg ccgggcacga cgcgcaggta ctcgtcggga aactggtcag gggcgatccg
     4861 ttctgcgcct tcgggtgggg agtattgcaa cttcactccg gtatcgaacg acaccttgaa
     4921 cactctggtg tcggtgttct catcgttttt gcatttgacg gacccaatga cccttccgtg
     4981 gtccatcttc atctcgccgt cagtctgcgt aatcccgaag ccttcggccg cgcccgattc
     5041 cagcaaaaac tggtgcacaa acgaatgctt gcctgacgct tcgaaggtca ggcagatccc
     5101 tcgaatcttg tcggagccgt gatactcgtt ctgaaggatg ctcgtcagca cagcttcgga
     5161 caacggcaga ttggtgatca tcacctcgat gatcccgcac gacagttcat cgacactgtc
     5221 aacgttgaat ggtttcgtgt ccatcggcca tgcctcacgc ttgcgtccgt aaatgtggcg
     5281 cagcgtgatg gcttttctat tgagcgtgag cgtgcccgtc acggtgccgt tgtcggcaac
     5341 ggcggttggg ttgcccggcg tggtagtcgc gtgttttggc gcggccggcg cagacgcttt
     5401 cgcgttcggc gctaccgacg tcatgacgca caacgcgagg agcatcccgg ctacagggat
     5461 cgagtaatca ggaagaacgc cgggatgaat cgaccgatgc ttcttcatat tcaacccctc
     5521 tttccgcgac gctcccctcg atgatcaacc ccgcagtaac agcggcctga aaaggtaatc
     5581 aagcactccc cgcagaatgt agatcgccag cagaagcagc agcggcgacc agtcgatgcc
     5641 acccatcttg tgcggcggca cgagacggcg gaacggacgc aggaacgggc tggtcatgcg
     5701 cgtgaagatg tcgttgagct gattgagaat cacataggcg ctcttgtagc gccagcggaa
     5761 cgacgtctgc gacgcgaaga agagaaccca ggagaggatc acccacacca ggacgatcca
     5821 ctggatcacc tgcaggacga tcatcagcgc gtcgtagaac accaccagga ttcccatcta
     5881 tcctcggctc ccgaagatcg cgcggccaac gcggaccatc gtcgatcctt cctcgatcgc
     5941 tgcttcgaag tcttcgctca tgcccatcga cagttccttc agaccaagcg catcgcgcat
     6001 cgaccgcagc tcccggaagt atggacgcga ctcgtccgga gtgccaaccg gcgggatggc
     6061 catgagtccg atgagccgga gcggagccaa cccgcccacc tgtttcgcga tgacatcgac
     6121 atcctcgcgc ccgaggccgc tcttctgcgg ctcgtcgccg atgttgacct gcaacatcac
     6181 gtcgatcgtt ttgccctgac cctcggccgc gtgggcgagt ttctcggcga gatcgagcga
     6241 gtcaaccgcc tcgatgacgt cgaacaactt cacggcgtct ttcgccttgt tcgtttgcag
     6301 atgtccgatg aggtgccatc ggacgctgcc gtgcaccagc ggcttcttgt cgcgcgcttc
     6361 ctgaacgcgg ttctcgccga tgtcggtgat gcctgcggca atcgcctcgt cgacaaactc
     6421 agccgggaag gtcttcgaca cagccacgag ctttacgtcc tcgcgtgcgc gtcccgcgcg
     6481 aacgcatgcg cgggcgatac gctcttcgac ggcggcgatg ttgtcacgaa tcgacacggc
     6541 gtctctcgat ccaccgatcg ttcggcgtgc gctcgggacg gtcgagcgcg gcgttggcga
     6601 gcctgtcggc gtctttgttg cccgcgcgca gcgtatggcg gatcttgaac ttcgggaaac
     6661 gacgcgtgat cgatttcgcg cgctcgtaca gaggcacgag gttgggatgc ttgacgcggt
     6721 acttgccgag catctgctgc acgagcagga gcgagtcgga gacgatctcg acgtcggtcg
     6781 cgccttccat ctgcgcggtc gacagcgctt cgaggaggcc ggcgtactcg gccacgttgt
     6841 tcgtcgtcgt gccgagaaag ccggagatct cgatgatctc gctatcgtcc ttgcgcatgt
     6901 agacaccgta ggaagcgggt ccgggattgc cgcggcaggc gccgtcgacg aacgcgatga
     6961 tcttcaggat gggggactct ccagatagag gatacggctg cagccttcgc agtgaacgag
     7021 atcgccgcgc ttgagctgtt gcagcacggc cgggcggaca cgcgtgcggc agccgttgca
     7081 ggagtcggcg acgacgcgcg cgatggcgac gccctgccgc tgtttgaaga tgcggtggaa
     7141 ctcgcggctc aggcgctcgg gtagcgatgc ctcgatcttt gcggcgcggg cctgaagctg
     7201 cccgacctcc ttgcgaagat cgccgaggga gtgttgccag gcttcgtgct cggcatccca
     7261 gcgggacttg agctcgacgc tgccggcggc cagatcatcg ttctgcttct gcagcccatc
     7321 gacgtccgcc atcgtcttga gcgtcgagtc ttccagctct ttgacctgct tgcgcgtggc
     7381 gtcgatctct ttccacgccg ccgcgtactg ctgctggttt ttcacctgca tgagctgccc
     7441 ttgatacttc ttgaggagct cctgctgatc tgacaactcg ccgtcgacgc gccgccgttc
     7501 gcgggaatgc tgctcgatct gctgctgcag cttctccatc tcggcattca cgctctgcca
     7561 ctcgcgatcg acggccgcga aggattccgg cttgttcgtc agttgttttt cgcggtctcc
     7621 gagcgccgac aggactgttt gcaactccca aagtttttca acgtccggat tcaaccaggc
     7681 ctccaaaaac gaaaaaggcg ttccggtgat ggaacgcctg tgcggtaaga aattgcccgg
     7741 ccggccgctc gagcatcgtc aggagcgccg gcgcggaaag ggtgagcgat cctccggcgg
     7801 gtccgcgccg gtagtgcagt cgatcgttcg tcatattcag gatgtcaacc atcagtttcc
     7861 tggtgggtcc accaggattc gaacctggga cctgccggtt atgagccggt agctctaacc
     7921 gctgagctat ggacccggac ggatgattct aacaaaaggg agtgcggagt cgagggtttt
     7981 cctgaagagc gccgcacgaa gagtgaagag tgaaagaaag gagggttact tccttccact
     8041 cttcatcgta tttcgacgtg atcgccgatc ttcatcgagt agcgcatggc cacgatcttt
     8101 ccggtcgacg tgtgcgtgtt cgtcgtcagg atgccgacct cgccgaggac ctggcgcggc
     8161 tggccgggcg caacttcctc agtgtagacc gtgaggaaat cgccgggctg gagctgatcg
     8221 gcctggccga ggttcacttc gacgagcagc ccttctccga gagcagcgtc ccacccaccc
     8281 tgcgcaccga cgatgtagcc gttcttcttg ttgctggagt tgtcgcagaa cgcaggcatg
     8341 tcggggatcc gcgccaacgg aatcggaatc tgcggcagcg gcttgaggtg cgtgccgacc
     8401 ggaacctccg agcacgactg cgtaatgaga ccgcgcgcct gatgctcgtc ggcacagaga
     8461 attcgcacct ggccgcgata aaggtactcg cgcccgacgt tctgcgtgcg tttggcggga
     8521 tggctgatca gacggccaac atccacgaga atgtacgtct cgccggccac aaggccgctg
     8581 gctgtgccgc ctttgaggat gacgagatca ccaaccgctc cgctgatgac ctgctcgacg
     8641 gcacccggcg catagcgcac ttccgcgtct tcgaaagcca ggatcgtgtt cggcatcgac
     8701 tcgccgtctt cgccgatgta gccatagcaa tagacatcgg cgtctgtgcc gagtggtacc
     8761 gggttccggc tgccgccggc ggcgtcggcc gcggatatgt agccctcgcc ccccggaaac
     8821 gcgttgcctg acgttgtgtc actggacatc gaaggagcgg actcgggcat cagcgccggt
     8881 ggcggtggga gtgtcgaatc atggccgccc ccctgcgcct cagagatctg ctgcgcggtc
     8941 tcggagtcga tcaacagcac gtcgccggga tagatccaat gcgcatcggt gatccaggtg
     9001 ttgccttccc aaagctgcgg ccacatgtaa gcgttcttga agaactgctc cgccagactc
     9061 cagagcgtgt cgccgcgttt gatcgtgtag gtcttcgatc cggcgggcca ggtggcggga
     9121 tctggagggt tgtacgccgt ccaatggtcg cccacgcgat ggagatcgcg cggcgggtgc
     9181 gatgaggact tcttcggcgc cccgaaaatc gggatagcga ccgcaagcag gagaacggga
     9241 accaccctac gcacactgac cattttcccc tcccaaggtt gtcgaggtcg g
//
LOCUS       DATLVW010000360         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_593236, whole genome shotgun sequence.
ACCESSION   DATLVW010000360 DATLVW010000000
VERSION     DATLVW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593236"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..2064
                     /gene="ltrA"
                     /locus_tag="VNN25_10970"
     CDS             625..2064
                     /gene="ltrA"
                     /locus_tag="VNN25_10970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HXH92092.1"
                     /translation="MGVKRQQGSGDQKDLFDQALQASLRLGATGEGGTGPGACAEPQS
                     LTVWDQQRALTQTLMEEVASSANLNQAYKRVKANKGAAGVDAMSIAELLPWIREHRER
                     LIASLIDGSYQPQTVRGVEIPKPGGGKRQLGIPTVVDRLVQQAILQVLEPILDPTFSS
                     SSFGFRPGKSAHQALAQAQTYVADGREIVVDLDLEKFFDRVNHDILMARLAWRIGDKR
                     LLRIVRRFLEAGMMQAGVCIERREGTPQGGPLSPLLANLLLDDLDKELERRGHRFCRY
                     ADDCNIYVRSQAAGERVMASVVAFLEGKLRLKVNRAKSAVAPVVERSFLGHRLLPGGR
                     LGLAPKSLDRAKERLRRITRRNRGIALERMIAEVNSFTTGWVTYFRHAACKSALRDID
                     DWLRRKLRCVRLKQCKRVKSIADFLVKGGVPARRAWPLALSGKGWWRRSGTPPANEAM
                     TLQWFAKLGLVSLQAQHAALQSAGNRRVR"
BASE COUNT          496 a          823 c          871 g          414 t
ORIGIN      
        1 acccgaacgc cggctggaag ggccgcgccc tttggtcgac cgacggcaat cggacgccgt
       61 tccatcgcga aaccggcaag ggaaccctgc ccaagatcgt gaagttccag gttaggcccg
      121 atccactcgc gcactgagcc cgatatcggt cgttcaaacg acctgccacg cgaatgcccg
      181 gctctgcgcc gggcattcgt attagtgtgc caggcatggc gctgatgctg gaggtggaag
      241 tcctctgcgg gccttggtga tgggaaccgc gagccgaagg caactgcgtc gccgcgaggc
      301 ggggtgggga ggaagccgga ggccaagttc ggagccgacg aacagaaatc ggatagaagg
      361 ccggcgcggt ggggcaaccc ggctatggac ggggaacgcc cgatagtcac ccggacacac
      421 gcgcaggtaa atccggcggc gcccgagcga agcagcaacg tcttaccctg ggagacctct
      481 gcgcctctcc gggcaaccgg agcagggtgt cggcaacgac acctgacggg cggagaggag
      541 tcagccgagg ccgtagtagc cggacggcgt accgcccggt gaagggccga attcagtagc
      601 caaggagcag tctgagccaa ctcgatgggc gtgaagcggc agcaaggcag cggcgaccag
      661 aaggacctgt tcgaccaggc cctgcaggcg tcgctgcgcc tcggcgcaac cggcgaaggc
      721 ggaaccggac ctggtgcgtg cgcggagccg caatcgctta cggtgtggga ccagcaacga
      781 gccttgacgc agacgttgat ggaggaggtc gcgagttcgg cgaacctcaa ccaagcctac
      841 aagcgggtca aagcgaacaa gggcgcggcc ggggtggatg cgatgtccat cgcggaactg
      901 cttccttgga tcagggagca ccgcgagagg ctgatcgctt cgctcatcga cggcagctac
      961 caaccccaaa ccgtgcgcgg ggtggaaatc cccaagcccg gcggcgggaa gcgccaactc
     1021 ggcatcccga ccgtggtgga ccgcctggtt cagcaagcga ttctgcaagt cctggagccg
     1081 atcctcgatc cgaccttctc gtcatcgagc ttcggtttcc gcccgggcaa gagcgcccat
     1141 caagcgctcg cccaggccca gacctacgtt gccgacgggc gcgagatcgt cgtggatctc
     1201 gatctggaga agttcttcga ccgggtcaac cacgacatct tgatggcccg cctggcgtgg
     1261 cgcattggcg acaaacgcct gctgcggatc gtccgccgct tccttgaagc gggaatgatg
     1321 caagccggcg tgtgcatcga gcgacgggag ggtacgcccc agggcggtcc tctgtccccg
     1381 ctgctcgcca atcttctgct cgacgacctc gacaaggagc tggaacggcg cggccaccgc
     1441 ttctgccgct atgccgacga ctgcaacatc tatgtgcggt cacaggcggc gggagagcgg
     1501 gtgatggcct cggtcgtggc cttcctcgaa ggcaagctcc gcctcaaggt caaccgggcg
     1561 aagagcgccg tcgctcccgt ggtggagcgg tccttcctcg gacatcgtct cttgcccggc
     1621 gggcggctcg gtctcgcacc gaaaagcctc gatcgtgcaa aagagcggct tcgacgcatc
     1681 acccggcgca accgggggat cgcgctcgaa cggatgatcg cggaggtgaa cagcttcacc
     1741 accggctggg tgacgtactt ccgccacgcg gcctgcaaaa gcgcactcag ggatattgac
     1801 gactggcttc gtcgcaagct ccgctgcgtg cgtctcaagc aatgcaaacg tgtcaaatcg
     1861 atcgccgact tcctcgtgaa gggcggcgtg ccggcacgca gagcatggcc gctggcactc
     1921 tcgggcaagg gatggtggcg tcggtcgggc actccgcctg ccaacgaagc catgacgttg
     1981 cagtggtttg cgaaactcgg gctcgtcagt ctccaagccc agcacgccgc gctgcaatct
     2041 gctggaaacc gccgagtacg gtgagtacgc tcggtggtgt gagaggggga gaccgcgagg
     2101 aatcccccta ctcgattggt cgagcaactc cgcgctacgc agggttcagc tctgtttcga
     2161 ccgcttcgac taaattgacg aggctgcggc caaacgcaac ccgaattcgt tgtctcagcg
     2221 tcaggaccgc aagctttggg atccgctgag cgcggccgcg ctgctccttg actcgccagc
     2281 gagtactgct caggtagaca gcggaggact tgttcatccc gagtgtcgta accacgggaa
     2341 ggagataccg cgcctggtgc cggcgcgacc cctgcgcagc cgcgaccagg ctcgcgaagg
     2401 cctgcagctc cctcgcttcg attggagcga atggggcttg ttcatcgtga tggcgaacca
     2461 gcgccgtgcc ccgcccgcgc ggggctcgat agtcatgccg cggtgacgaa gggtcgcaga
     2521 atttcgcctt gctgcatcga cccgtctgca tatacaccgc gcgcctcgga gatggccggt
     2581 agagagcgcc cggaggaaac tcca
//
LOCUS       DATLVW010000361         4687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_593252, whole genome shotgun sequence.
ACCESSION   DATLVW010000361 DATLVW010000000
VERSION     DATLVW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593252"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VNN25_10975"
     CDS             complement(<1..1292)
                     /locus_tag="VNN25_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXH92093.1"
                     /translation="MRPYDDEDTDDFDRTLSLRRLPESVTTFSDKRTSAERRGSSNSR
                     ILFITVVVIAILYAAKPVCVPIALAILFAFLLTPIVSILERTPLRRTGAVVLSLSLIV
                     TGLGFGGWWLYQQFNDVAKEFTDAATSGKIEQKLRFLKRSPGGTIDRLEKTLARVTEA
                     TADKQEKADLKVRVIPDRQNVAGRYKTLAPEIELVAASFLVIVLVFFLLQDREQLRDK
                     MLRLAGRAHLTVTTQAIGETSDRISRYLLTIALLNVGFGVLIGLGLFLLKVPHAPLWG
                     VLAGLFRFIPYVGAVLSAALPTFLSVAVFPNWYIPLAVLALFVLTDQLIGGFIEPIVV
                     GHRVGVSPIALLVSAIFWGWLWGPVGLLLAVPISVCLTVAGEFIPALRIFSIMFGAED
                     PLEGYLSFYNRLLLRDRTGAIAIADRHSETDTMEEMFS"
     gene            complement(1372..1602)
                     /locus_tag="VNN25_10980"
     CDS             complement(1372..1602)
                     /locus_tag="VNN25_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92094.1"
                     /translation="MSKDPSESQDLEIPETDLSGAVRGKYYDRAMAGTNIVLLEPDVA
                     RVFRDSAVVSQALREYLNEHGAPPELPAAKAG"
     gene            complement(1586..1861)
                     /locus_tag="VNN25_10985"
     CDS             complement(1586..1861)
                     /locus_tag="VNN25_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015196187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXH92095.1"
                     /translation="MEYEWDPDKDSANQRLHGVSFAIASTVFLDPFAATVPDERFAME
                     EFRFRTTGYTLTNRLVIVAHTDRGDRIRIISAREVTARERRQYEQRS"
     gene            complement(1968..3890)
                     /locus_tag="VNN25_10990"
     CDS             complement(1968..3890)
                     /locus_tag="VNN25_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92096.1"
                     /translation="MRRLLITLTILFIALPLCAQWRRANLYGADVRALIIDPSNPDTL
                     YLGTSGGEVYVSTDAAKTWQNPRRGIPFPGYVVDNLVLDRSGRLWAACWGAWGGGVIA
                     VSEDGGQNWTRRDAGLEDFSVRAIAIDPHDADFIVVGGLTGVYRSTDGGASWEQISNQ
                     TNVESLALDPRTHDRIYVGTWRQGTRTDDGGKTWKLINNGMVLDTDMFSITIDRYNPD
                     KVWVSTCGWVYNTPNRGDTWTRYRDGFDNRRIHDIELDPCDRDSIYAGSVAGLYRSHD
                     GGKSWYVVSDESLVVNTIALHPQRPGRVILGVEGDGVYLSNDNAKTFTRMCDGLRNLT
                     ITSVASDASHPKNVYAAVAFGGASSGIYRSVDAGASWAKLASIDLPPVLSLVVSDDAD
                     AKFVAGTEKGFFWSSNGEHWTQAQPAAFPIRVDKVLRFNKARYFAATSEGVFTSRDSG
                     KSWYLLAGADNRAVDMAIGNIGDKRALFALTSVGVLAFDGTQWISIKGAPSKGRTLAV
                     RGSGDDQLVFVAGSQGVKAGRVGADRKWVEAEAPDAQFASVFGSSRSSDNFVFLTSRQ
                     QHEMLVAEPKTEEWRSFPLPSRTAEVTSVALDPFDGERLYVGTQGEGIFIFEGKSAKY
                     EAKKKATEAAAAMGTN"
     gene            4094..>4687
                     /locus_tag="VNN25_10995"
     CDS             4094..>4687
                     /locus_tag="VNN25_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXH92097.1"
                     /translation="MTALRTCVFPCAGFGTRFFPATKVIPKEMLPLVDKPILQYGIEE
                     ALGSGLDKLIVVMSKGKDAILDHFDRTAELERSLQERKKNDLLSEVETVSTMADLISV
                     RQKEALGLGHAVLQAKDAVGNEPFAVVLPDDVILAGEPCLLQMRRVFEETRRPVIALM
                     EVSAEETTRYGIVAGNMLGNRRFHITDMVEKPKVNPPS"
BASE COUNT          928 a         1445 c         1470 g          844 t
ORIGIN      
        1 tcggagaaca tctcctccat cgtgtctgtc tcggaatgac ggtcggcgat ggcgatagcg
       61 ccggtgcggt cgcgcaggag cagccggttg tagaagctga gatatccctc cagcggatct
      121 tctgcgccga acatgatcga gaagatgcgc agtgccggga tgaattcacc agcgacggtg
      181 aggcagacgg agatcggcac cgccagaagc agaccaaccg gtccccacaa ccatccccag
      241 aagatggccg atacgagcaa tgcgatgggc gagacgccga cgcgatgtcc gacgacgatc
      301 ggttcgatga agccgccgat gagctgatcg gtcagaacga agagtgcaag aactgccagc
      361 gggatgtacc agttgggaaa aaccgcgaca gaaaggaacg tcggcagggc cgcgctcaac
      421 accgccccga cgtacgggat gaaacggaac agtcccgcca gcacgcccca cagcggcgcg
      481 tgcggaactt tcagcagaaa gaggccgagc ccgatgagca ctccgaagcc gacattgagc
      541 agagcgatgg ttaggaggta gcggctgatg cggtccgagg tttcgccgat ggcctgagtg
      601 gtgacggtga gatgcgcgcg gccagccagg cgcaacatct tgtcgcgcaa ctgctcgcgg
      661 tcctgcagca gaaaaaaaac gagcacgatg acgaggaagg acgcagcgac gagctcgatc
      721 tccggtgcca gcgtcttgta acggccggcg acgttctgcc gatcggggat cacgcgcact
      781 ttgaggtcgg ctttctcctg cttgtccgcg gtcgcttcgg tgacgcgcgc gagcgtcttc
      841 tcgagcctgt cgatcgtgcc gccgggactc cgcttcagga agcgaagctt ctgttcgatc
      901 ttgccgctcg ttgccgcgtc ggtgaactcc ttggcgacgt cgttgaactg ctgatacaac
      961 caccaacccc cgaacccgag tccggtcacg atgaggctga gcgagaggac gacggctcct
     1021 gtgcggcgta agggagtccg ctccagaatc gagacgatcg gcgttagtag aaacgcgaac
     1081 aggatcgcca gagcgatcgg gacgcacacc ggtttggcgg cgtaaaggat ggcaatgacg
     1141 acgacggtga tgaagaggat gcgcgagttc gacgacccgc gccgctccgc cgaggtgcgc
     1201 ttgtcggaaa acgttgtcac agactccggc aagcggcgca gggaaagcgt ccgatcgaag
     1261 tcgtcggtat cttcgtcgtc gtagggacgc acgttgcgat gagtctactg caagggggaa
     1321 gccggggggc ggccgggcga acaacggacg cctgtcgcct gctgcgaacg gttagcccgc
     1381 cttagcggca ggcaattctg gcggcgctcc atgctcgttc agatattcgc gaagcgcttg
     1441 actaacaacg gcagaatcgc ggaacacccg agcgacatcc ggctcaagca gaacgatatt
     1501 ggttccagcc atggcacgat cgtagtactt gccccgtacc gctcccgaca gatcagtctc
     1561 gggaatctcc aaatcttgcg actcgctagg atctttgctc atattggcga cgttctcgcg
     1621 cagtgacctc gcgcgccgaa ataatgcgaa tgcgatcacc gcgatcggtg tgtgcaacga
     1681 tgacgagacg attcgtcagc gtgtaccccg ttgttctgaa tctgaattct tccatcgcaa
     1741 agcgctcatc cggtacggtt gcggcgaacg gatccaagaa taccgtggat gctattgcaa
     1801 acgaaacgcc gtgcaggcgc tgattggcgc tgtctttatc gggatcccac tcgtactcca
     1861 cgccccgagt atatgaccgc gatgttgctc ggatcggagc agttcgttgc gtgcgcggca
     1921 cacgggaaac tctgccgggc taggtcattg ctccgatgca aacgacccta attcgtcccc
     1981 atcgcggccg ccgcttccgt cgctttcttc ttcgcttcgt acttggctga cttaccctcg
     2041 aagatgaaga tcccctcgcc ctgggtaccg acgtacagac gctcgccatc gaagggatcg
     2101 agggcgaccg acgtcacctc ggcggtgcgc gaaggcagcg ggaacgagcg ccactcctcc
     2161 gtcttcggct cggcgacgag catctcgtgc tgctggcgcg aggtcaggaa aacaaagttg
     2221 tcactgctgc gcgagcttcc gaagaccgag gcaaactgcg cgtcgggggc ctcggcttcg
     2281 acccatttcc ggtccgcgcc gacgcgtccg gccttcacac cctgcgagcc ggccacgaag
     2341 acgagctgat cgtcgcccga gccacgcaca gccagcgtgc gtcccttcga cggtgcgcct
     2401 ttgatcgaga tccattgggt tccgtcgaag gcgaggacgc cgacgctggt caaggcaaag
     2461 agagcgcgct tgtcgccgat gttgccgatg gccatgtcga cggcacgatt gtcggcaccg
     2521 gcaagcaggt accagctctt ccccgaatca cggctcgtga agaccccctc ggaagtcgca
     2581 gcgaagtagc gtgccttgtt gaatcgcaag accttgtcga cacgaatggg aaatgccgcc
     2641 ggttgcgcct gcgtccaatg ctcgccgttg ctgctccaga aaaatccttt ttcggttccg
     2701 gcaacgaact tcgcgtcggc atcatcactg accacgagcg agagcacggg cggcagatcg
     2761 attgacgcca gcttcgccca gctcgcacca gcatcgacgg agcgatagat gccgctcgac
     2821 gcgccgccga acgccaccgc ggcatacacg ttcttcgggt gtgacgcgtc ggaagcgacc
     2881 gatgtgatgg tcagattgcg aaggccatcg cacatgcgcg tgaatgtctt cgcgttgtcg
     2941 ttggagagat aaacgccgtc tccctcgacg ccgaggatga cgcggcctgg ccgctgcgga
     3001 tggagcgcga tcgtgttcac aaccagcgac tcgtccgaga cgacgtacca gctcttgccg
     3061 ccgtcatggc tgcgatagag accggccacc gagcctgcgt aaatcgagtc gcggtcgcac
     3121 gggtcgagct cgatgtcgtg aatgcgccgg ttgtcgaaac cgtcgcgata acgcgtccag
     3181 gtgtctccgc ggttcggcgt gttgtagacc cagccgcacg tggagaccca aaccttgtcg
     3241 gggttgtagc ggtcaatggt gatcgagaac atgtcagtgt cgagcaccat cccgttgttg
     3301 atcagcttcc atgtcttgcc gccgtcgtcc gtgcgcgttc cctgtctcca cgtaccgacg
     3361 tagatgcggt cgtgcgtccg cggatcgaga gcaagcgact cgacattcgt ttgattcgag
     3421 atctgctccc acgacgcgcc gccgtccgtc gatcggtaga cgccggtcag gccgccgaca
     3481 acgatgaaat cggcgtcgtg tggatcgatg gcgatggcgc gaaccgagaa gtcctcgagg
     3541 ccggcatcgc ggcgcgtcca gttctgcccg ccatcctcgg agaccgcgat gacgccgcct
     3601 ccccacgccc cccagcacgc tgcccagagg cgcccgctgc ggtcgagcac gaggttgtcg
     3661 acgacgtagc ccggaaacgg gattccgcgg cgcgggttct gccacgtctt cgccgcgtcc
     3721 gtcgaaacgt agacctcgcc gccgctcgtg ccgaggtaga gcgtgtcggg attggaagga
     3781 tcgatgatca gcgcccgaac gtcggcgccg tacagattgg cgcgccgcca ctgtgcgcaa
     3841 agaggtaacg caatgaagag aatcgtgaga gtgatgagga gtctacgcat gcccgcccag
     3901 ttccggaaga gttttaccac gaggcggtag caatattgct gccgtggggg gtggggagta
     3961 gggggtgggg tgtgggagat ggggttcgca gcgtcttgct cgcgcacgac gtctgaaccg
     4021 cgcccgatct tctgcctctc tcccactccc cacccccaac tccccacccc ccacactgct
     4081 atcctcgcaa atcatgaccg cccttcgcac ttgcgtcttc ccctgcgccg gattcggcac
     4141 gcgcttcttt ccggcgacga aagtcattcc gaaggagatg cttccgctcg tcgacaagcc
     4201 gattctgcag tatggaatcg aggaggccct tggatccgga ctcgacaagc tgatcgtcgt
     4261 catgtccaag ggaaaagacg ccatcctcga tcatttcgat cgtacggcgg agctcgaacg
     4321 ttcgctgcag gagcgcaaga agaacgatct cctcagcgag gtcgagacgg tcagcacgat
     4381 ggccgatctc atctcggtcc ggcagaagga agcgctcgga ctcggccacg ccgtgctgca
     4441 ggcgaaggat gccgtcggca atgagccgtt cgccgtcgtc ctgcctgacg acgtcatcct
     4501 ggccggcgag ccctgcctcc tgcagatgcg gcgcgtcttc gaggaaacgc ggcggccggt
     4561 catcgcgctg atggaagtgt cggcggaaga gaccacgcgc tacggcatcg ttgccggcaa
     4621 catgctcggc aaccgacgtt ttcacatcac ggacatggtg gagaagccga aagtgaatcc
     4681 gccctcg
//
LOCUS       DATLVW010000362         8030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_183406, whole genome shotgun sequence.
ACCESSION   DATLVW010000362 DATLVW010000000
VERSION     DATLVW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183406"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(681..3182)
                     /locus_tag="VNN25_11000"
     CDS             complement(681..3182)
                     /locus_tag="VNN25_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92098.1"
                     /translation="MRKLLPIVVILIVSSFLTSCKLKPLPGHVKDEALLAGRDAASLP
                     GADEDYYADMDYGLTKNPEAIRASLDPYVPGITADAAVKAAVIGRNNWIVWTAGNDHL
                     WDTLSVSSFGNLDLLKTISSHPKLKNSRDNRWHYLGLVNEPCFRKATGPRADRWGLWL
                     DERVEGPGCGPDPFENEQKYPGVKIGARGTGKLALGSYYGYATGVVGLRLFPNPAFDE
                     AAQKKWDPVRYYNDKSYYNDANLVKPFRVGMSCGFCHVGPNPSNPPADPENPEWANLN
                     SNPGAQYFWIERIFMFDQDYGSFIWQLFHTSRPGALDTSLVSSDEINNPRTMNAIYNL
                     GARLEIARKWGHEELGGGGLNNKQFNEYVPAGTALTKFYDPTTHTVNTPHVLKDGSDS
                     VGALGALNRVYLNIGLFSEEWLLHFIPLVGGPKVSPIKIADAEKNSSYWNANAAQTPD
                     VALFFLATAKPDYLAVAPGGSRYLEDSDLVPRGKEVFGERCARCHSSKLPAKAFTNFF
                     QPGCIGPNYLKCWNDYWNWTKTAEFKTEMKEIVKSRDFLDNNYLSNEVRVPVTLLETN
                     ACSPLATNAIRNNIWDNFSSESYKNLPSVGKVMIHHPYTSEPSWYDMPAGGRGYTRPA
                     SLISLWSTAPYLQNNSVGHFEESGSVPDRMKAFEDGIEQMLWPEKRKGNGTFATQSGK
                     SLPGWIDKTTQTSYLNIAPGYVPSELKPLVGLAAGLHPNIFYEGGIRIGPIPEGTPVG
                     MLSNIDLQNPGEVLILLLKIKHKLDALPENASDDEARKVFAPLVPELLKASKCADFVV
                     NKGHYFGTDYLPASEGEPGLSNADKRALIAFLKTF"
     gene            3445..5238
                     /locus_tag="VNN25_11005"
     CDS             3445..5238
                     /locus_tag="VNN25_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXH92099.1"
                     /translation="MGKAGLVFTVVLLAELGLAMGQEIPKLPPLDDPHARMEFANKRY
                     PSARVCGECHPTQYAQWSLSSHAYAAVSPMFNKFEQRINDLARGTINSFCVRCHATVG
                     TALGESREIAWWDRAGAAQEGITCVTCHRVGEAYSKSNGARRITPGDISEPVFGPFDT
                     TGVLKAISNAEIYHILVSSDQPDRAGYTRIHQQAIPSTVIKQSELCVTCHQVQVHAGI
                     KLETVWEEYRASPAFKEGTTCQDCHMSPVPGQKVAFTRGPAAIIPNGGTVNDDRPLSD
                     HTFVGPGYPISHPGLFPISLVPSRFTPQQWLKFDYRCRWGSDEFENKTAVLPGCDKAI
                     APEWQNAADRKEAWGVVVANLARWDERKKLRIELLEKGSKLDGPFFTSPLKVGQDFSF
                     DYKLTNLNKGHNLPSGSLGAQPELWLNVALIDPDGKRVWESGYVDSRGDFADLQSRDV
                     RERKISDQDPFKGEPLPFDAQLFSMGAKFITTNVKGTDREMFLPINLDFDQLPFIRPG
                     GTPSSVNNHPPNVRMEKHSIPPLATRTAHYKVPASKLTKAGKYRLAARIRGRTEPMYF
                     MDFVFATTDMERAENQWVTDTHQYAVEFEMH"
     gene            5252..6775
                     /locus_tag="VNN25_11010"
     CDS             5252..6775
                     /locus_tag="VNN25_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92100.1"
                     /translation="MKRKSSTANPFLAGLLLLAAAVPGAFAQEPPDSQQHNMSHEAKL
                     LPDPPAEGSFIPDPVYPQPYDAQAQLDIYGAKHLNPNPTGVPAVDLGIRMYDRGAYSP
                     RSTWLFGPKDPVQSGFITGGDLRIAADSYDSGTGGKKQSTVAARLNLDMDLALTATER
                     IHAFTRPLDKGGQFTRYDISGAVKDKFTSKFDFDLDTLFFEGDLGQITGGLQNHAASF
                     DLPIAFGRMPFVTQNGVWIEDAFDGAAFSFTAKNIPSLDVSNTDLTFFAGWAQLTTDA
                     APNSSRNRVLGLAGFADAHKGYIEYGYGYVKAEDDNLSYHNLTAAFSRRYFGRIANSV
                     RVIGNFGQKGDPGKAKTADGELFLFESSLTPKYLKLAEANQSNFVPYLNLFAGFNSPQ
                     SLARGADSGGVLRNTGITFESDGMTAYPTLDAKAHKSYGGALGLEYLFDLAHQIVVEG
                     SAVERMSGNTAGSQYGIGVRYQQPISPDLIIRFDAMKGWLQGQKDIYGVRAELRLKF"
     gene            6787..7254
                     /locus_tag="VNN25_11015"
     CDS             6787..7254
                     /locus_tag="VNN25_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXH92101.1"
                     /translation="MSVFQLNVNGKGYAVDIDGNMPLLWALRDILGLKGTKFGCGVAQ
                     CGACTVHLDGVAARSCSVDVGVACLSKITTIEGLSYDGELHPCQKAWVELGVPQCGFC
                     QPGQIMTASALLKANPHPTDEQIDAAMRGNICRCGTYPRIRAAIHKAAGDATS"
     gene            7251..>8030
                     /locus_tag="VNN25_11020"
     CDS             7251..>8030
                     /locus_tag="VNN25_11020"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH92102.1"
                     /translation="MSTKHRVSRRAFLQTAGVGAGAVVFGSYLTPALQAQGLITNLIN
                     GFPVSGVPVVALKLNGDVVIIAHRPEMGQGIRSSLAAVLADEMEADWKRVTVKQADAE
                     AVKYAVIFPSEVPANYPLPFPYEEIPATQKPKVQYLVPPEGSQFADSSRSMAAFYTVM
                     RLYGAGIRLAMIRAAAKKWGVSATDCHAEKHRVYGPGGRSSDYRWLLLGAGKETPTYE
                     EINAALKKPEEFRYIGNGKEKMPFVDASDMVQGKAVYGADVD"
BASE COUNT         1617 a         2489 c         2411 g         1513 t
ORIGIN      
        1 cgccgccgcg cagaccctgt gcaaacatcg cgattcgcgc tggcggctcc ggctgtctca
       61 ggatgacacc aatttcggtt actgccggac acacgagcgc gagcgtttgt ccggatcgcc
      121 tgacggaccc catcctgaga cgttttcggg gtcaaaacgt tccatttcag cggtagaaac
      181 atctaagtca cgcgattgca agtggttacg cgctgatcca ccaaagccga ggcctgatcc
      241 tgaatccaac tgaatacgta cacaaatgga atcaatgcgt tatcacaacg catgaatgcg
      301 aaattgcaac gcatgaatgc gaaaccagaa cgcgtgaatg cgaaatgaga acgcatgaat
      361 acgaagtcag aacgcatgaa tgcgaaatga gaacgcgtga atgcgaaatc ggaacgcgtg
      421 aatgcgaaat cggaacgcat caaggggaaa tcggaacctg tgaatgggca cttcggtggg
      481 atctggccca acgacgcatg cgaggaaggc ccaacgatcg acaaacgcga gatttattgc
      541 gcgttttcga catcgcggga ggcgtctttg tcctctccaa acgcgaagag aggcaacagt
      601 aagggcacca ggaatgtaaa aaatactcgc gccgccgccg gatcttgaat ccgacggcgg
      661 cgcgcaaatt caaaccttgc tcagaacgtc ttcaggaacg cgatgagcgc acgcttgtcg
      721 gcgttgctca ggcccggctc tccctcgctg gccgggaggt agtcggttcc gaagtagtga
      781 cccttgttga ctacgaagtc ggcgcacttg gaggctttga gcagttccgg cacgaggggc
      841 gcgaatacct tgcgtgcctc gtcgtcgctc gcattttccg gcagtgcatc gagtttgtgt
      901 ttgatcttca acagcagaat caggacctcg cccgggttct ggagatcgat gttcgacaac
      961 atccctaccg gcgtcccctc ggggatggga ccgatgcgga tgccgccctc atagaagatg
     1021 ttgggatgaa gacctgccgc caggccgacc agcggtttca gctcgctcgg gacatagccg
     1081 ggagcgatat tcaggtagct ggtctgggtg gtcttgtcga tccaacccgg caacgacttg
     1141 cccgattgcg tcgcgaacgt gccgtttcct ttgcgtttct ccggccagag catctgctcg
     1201 atgccgtcct cgaatgcttt catcctgtct gggacggaac cgctttcttc gaaatggccg
     1261 acgctgttgt tttgcagata gggcgcggtc gaccagagac tgatcagcga tgccggccgc
     1321 gtgtatccgc gtccgcccgc cggcatgtcg taccacgatg gctcgctcgt gtacggatga
     1381 tggatcatta ccttcccgac cgagggaagg ttcttgtacg actcggagga gaagttatcc
     1441 cagatgttgt tgcggatcgc atttgtcgcc agggggctgc aggcgttcgt ctccagcagc
     1501 gtgaccggca cgcggacctc gttggagagg tagttattgt cgaggaagtc gcgtgacttg
     1561 acgatctcct tcatctccgt cttgaactcg gccgtcttcg tccagttcca gtagtcattc
     1621 cagcacttca ggtaattcgg accgatgcag cccggctgga agaagttggt gaaggccttc
     1681 gccggcagtt tgctggaatg gcaacgcgcg caacgctcgc cgaagacttc cttgccgcgc
     1741 gggacaagat cggagtcctc gaggtacctc gatccacccg gcgccaccgc cagatagtcc
     1801 ggcttcgcgg tggcgaggaa gaagagcgcc acgtctggcg tctgggcggc gttcgcattc
     1861 cagtagctcg agtttttctc ggcgtcggcg atcttgatcg gactaacctt cgggccaccg
     1921 accagcggga tgaagtgcag cagccactcc tcgctgaaca gcccgatgtt gaggtagacg
     1981 cgattgagtg cgccgagggc gccgaccgag tcggagccat ccttgagcac gtgcggtgtg
     2041 ttcaccgtgt gcgtagttgg gtcataaaac ttcgtgagcg ccgttccggc ggggacatat
     2101 tcgttgaact gtttgttgtt cagaccgccg cccccgagct cctcgtgtcc ccacttccgc
     2161 gcgatctcca agcgggcgcc caggttgtag atggcgttca tcgtgcgcgg gttgttgatc
     2221 tcgtcggagg agaccagcga cgtgtcgagc gcgcccggac gcgaggtgtg gaagagctgc
     2281 cagatgaagc tgccgtagtc ctggtcgaac atgaagatgc gctcgatcca gaagtactgt
     2341 gcgccgggat tgctgttcag gttcgcccac tccgggttct ccggatcggc gggcgggttg
     2401 gatgggttgg ggccgacatg gcagaagccg cacgacatgc caacgcggaa cggcttcacc
     2461 aggtttgcat cgttgtagta gctcttatcg ttgtagtagc gcaccggatc ccacttcttc
     2521 tgcgccgcct catcgaaggc cgggttgggg aagagacgga ggccgacgac gcccgttgcg
     2581 tagccgtagt acgagccgag tgccagcttt ccagtgcccc gtgcgccgat cttgacgccc
     2641 ggatatttct gctcgttctc gaagggatcg gggccgcagc ccggaccttc cacccgctcg
     2701 tcgagccaga ggccccagcg gtctgcacgc gggccggtgg ctttgcggaa gcatggctcg
     2761 ttgaccaggc cgaggtaatg ccagcggttg tcgcggctgt tcttcaactt cggatggctc
     2821 gagatcgtct tcagcagatc gaggttcccg aagctgctga ctgacagcgt gtcccagaga
     2881 tggtcgttcc ccgccgtcca tacgatccag ttgttccggc cgatcacggc ggctttcaca
     2941 gccgcgtcgg cggtgatccc gggaacgtac ggatcgagcg aagcgcggat ggcctccgga
     3001 ttcttcgtca ggccgtagtc catgtcggcg tagtagtcct cgtcggcgcc ggggaggctg
     3061 gcagcgtccc ggcccgcgag gagggcttcg tccttcacgt gtcctggcag aggcttcagt
     3121 ttgcaagagg tcagaaaact gctgacgatg aggatcacga cgatgggaag gagctttcgc
     3181 atggacggtg cctccttgca caaagatgag gagtgtgact acacaaatat agggagtgtt
     3241 actaggggag tgccacctga agatgctttc cctaccatca atattggtaa gtcgaggaaa
     3301 aaattaatct tgcgcaggcc attgacacct cgccgtttgt catgacagaa tttctgacac
     3361 atccaatttg aatccgtaac tcgactcgac ccgtcgcgac acgtttccac gaagcgacgc
     3421 gcagctttga cgaggaggag ctttatgggg aaagccggac tggtattcac ggtcgtcctg
     3481 ctggcggagc ttggcctggc gatggggcag gagatcccga aactgccgcc cctggacgat
     3541 ccgcacgcca gaatggaatt cgccaacaag cgatatccgt ccgccagggt ctgcggcgaa
     3601 tgtcatccca cgcagtacgc ccagtggagc ctctcctcgc acgcctacgc cgccgtctcc
     3661 ccgatgttca acaagttcga acagaggatc aacgacctcg cgcgcggcac gatcaactcc
     3721 ttctgcgtcc gctgtcacgc gaccgtcggc acggcgctgg gcgagagccg cgaaatcgcg
     3781 tggtgggacc gtgccggggc cgcgcaggaa gggatcacat gcgtcacctg ccaccgcgtg
     3841 ggtgaggcgt acagcaagag caatggggcg cggagaatta cgccgggcga catctcggag
     3901 ccggtgttcg gccccttcga tacaacgggc gtactcaaag ccatcagcaa cgcggagatc
     3961 taccacatcc tggtgagctc cgatcaaccc gacagggccg gctacacccg tattcaccaa
     4021 caggccattc catccactgt gatcaagcag tcggagctct gcgtgacctg ccaccaggtc
     4081 caggtccacg ccggtatcaa gctggagacg gtctgggagg agtatcgcgc gtctccggca
     4141 ttcaaggagg ggacgacctg ccaggactgc cacatgagtc ccgtcccggg gcagaaggtc
     4201 gcattcactc gcggacccgc cgccatcatc cccaacgggg ggaccgtcaa cgacgaccgg
     4261 ccgctctcag accacacatt cgtcggcccc ggctacccga tcagccaccc cggcctcttc
     4321 ccgatctctc tggtcccgtc tcgtttcacc ccgcagcagt ggctgaagtt cgattaccgg
     4381 tgccgctggg ggagcgacga gttcgagaac aagaccgccg tcttgccggg ctgcgacaaa
     4441 gcgatcgcgc ccgagtggca gaacgccgcc gaccgcaagg aggcatgggg cgtcgtcgtc
     4501 gccaatctgg ccaggtggga cgaacgcaag aagctgcgca tcgagcttct cgaaaagggc
     4561 tcgaagctcg acggcccgtt cttcaccagc ccgctcaaag tcggccagga cttctccttc
     4621 gattacaagc tgaccaacct caataagggt cacaacctcc cctccggttc tctcggagca
     4681 cagccggagt tgtggctcaa cgtcgccctc atcgatcccg atggcaagag ggtttgggag
     4741 tcgggctatg tcgacagccg tggggacttc gccgatctgc aatcgcgcga cgtccgcgag
     4801 cgcaagatct ctgatcagga cccgttcaag ggcgaaccgt tgccgttcga cgcccagctc
     4861 ttcagcatgg gagccaaatt catcaccaca aacgtgaagg ggaccgatcg ggagatgttt
     4921 ctgccgatca atctcgactt cgaccagctc ccgttcatcc gcccaggcgg caccccctcc
     4981 tcggtcaaca accatccgcc caacgtgcgc atggagaaac acagcatccc accgctggcc
     5041 acccgcactg cgcactacaa ggttccggcg tcgaagctga ccaaagcagg gaagtaccgc
     5101 ctcgctgcac gcattcgcgg ccggacggag ccgatgtact tcatggattt cgtcttcgcg
     5161 acgacggaca tggagcgtgc cgagaaccag tgggtcaccg atacccatca atacgccgtt
     5221 gaattcgaga tgcactgagg agagggcgaa catgaaacga aaatcgtcca ccgcgaaccc
     5281 tttcctcgcc ggacttcttt tgctggccgc ggctgttccg ggcgcgttcg cgcaggagcc
     5341 tcccgactcc cagcaacaca acatgtcgca cgaggccaag ctcctgcccg atccgccggc
     5401 agagggctcg ttcattcccg accccgtcta cccgcagccg tacgacgccc aggcccagct
     5461 cgacatctac ggcgccaaac acctgaatcc gaatccgacg ggggttccag cagtcgacct
     5521 gggcattcgc atgtacgacc gcggcgccta ctcgccgcgc tccacctggc ttttcggccc
     5581 gaaggatccg gtccaatcgg gcttcatcac cggtggtgat cttcggatcg ccgccgattc
     5641 ctacgacagc ggcaccggtg gcaagaagca gtcgaccgtt gccgcccgcc tcaacctcga
     5701 catggacctt gcgctcactg ccaccgagcg catccacgcg ttcacgcggc cgctcgataa
     5761 gggtggtcag ttcacgcggt acgacatcag cggtgcggtc aaagacaaat tcaccagtaa
     5821 gttcgacttc gacctcgaca cgctcttctt cgaaggcgac ctcggccaga ttacaggcgg
     5881 cttgcagaac catgcggcca gcttcgatct gccgatcgcc ttcgggagga tgccgttcgt
     5941 cacgcagaac ggcgtctgga tcgaggacgc cttcgacggc gcggctttca gtttcaccgc
     6001 caagaacatc ccgtcgctcg acgtcagcaa caccgacctc accttcttcg ccgggtgggc
     6061 ccagctgacc accgatgccg ctcccaactc gtcgaggaac cgcgtcctcg gcctggccgg
     6121 attcgccgac gcgcacaagg gatacatcga gtatggctac ggctacgtca aagccgaaga
     6181 cgacaacctg agctaccaca acctgaccgc cgcgttcagc agacggtact tcggccgcat
     6241 cgccaacagc gtccgcgtga tcggcaactt cggccagaag ggcgatcccg gcaaagcaaa
     6301 aacagccgac ggagagctgt tcctgttcga aagctcgctc acaccgaagt atctgaagtt
     6361 ggccgaagcc aatcagtcga atttcgtccc ctacctcaat ctcttcgccg gcttcaactc
     6421 gccgcagtcg ctcgcacgcg gggctgattc cggcggcgtg ctgcgcaaca cgggcatcac
     6481 gttcgagtcc gacggcatga cggcctatcc gacgctcgac gccaaagcgc acaagtctta
     6541 cggcggcgcc ttggggctcg agtacctctt cgatctcgcc catcagatcg tggtcgaagg
     6601 gtctgcagtc gagcgcatga gcggaaacac ggctggctcg cagtacggaa tcggcgtccg
     6661 ctatcagcag ccgatcagcc ccgacttgat catccgcttc gacgccatga agggctggct
     6721 gcagggacag aaggacattt acggcgtacg agcagagctc cggctcaagt tctgaggagg
     6781 ttttcgatgt ctgtgtttca gctcaacgtc aacggcaagg gatacgcggt cgacatcgac
     6841 ggcaacatgc ctctgctctg ggctctgcga gacattctgg gactcaaggg caccaagttc
     6901 ggttgcggtg tcgcgcaatg cggcgcctgt acggttcatc tcgatggcgt tgctgcgcgc
     6961 tcctgctccg tcgacgtcgg cgtggcctgt ctctccaaga tcaccaccat cgaggggttg
     7021 tcgtacgacg gcgagttgca tccgtgtcag aaggcgtggg tcgagctcgg cgtaccgcag
     7081 tgcggtttct gtcagccggg ccagatcatg accgcctcgg cgctcctcaa agcaaatccg
     7141 caccccactg acgagcagat cgacgcggcg atgcgcggca acatctgccg ctgcggaacc
     7201 tatccacgaa tccgagcagc gatccacaaa gcggcagggg atgccacatc atgagcacaa
     7261 aacacagagt ctcccgcaga gcttttcttc agaccgccgg cgtgggcgcc ggagcggtgg
     7321 tcttcgggtc atacctcacc cctgcgctcc aagcccaggg cctcatcact aatttgatca
     7381 acgggttccc ggtctcgggc gtcccagtcg tcgccctcaa gttgaacggc gacgtggtca
     7441 tcatcgccca tcgccccgag atgggtcagg gcatccgctc cagcctcgcg gcggtgctgg
     7501 ccgacgagat ggaagcggac tggaagcggg taacggtgaa acaagccgac gccgaggctg
     7561 tgaagtatgc agtcatcttc ccgtccgaag tgccggccaa ctaccctctg ccatttccgt
     7621 acgaagagat tcccgctacg cagaaaccga aggtgcagta cctggttcct cccgaaggat
     7681 cacagttcgc cgacagctcg cgcagcatgg cagccttcta cacggtgatg cggctctacg
     7741 gcgccggcat ccggctggcg atgattcgcg cggcggcgaa gaagtggggg gtcagcgcaa
     7801 cggattgcca cgcggagaaa caccgggtat acggtcccgg cggccgttcc agcgactacc
     7861 gctggctatt gctgggtgcc gggaaagaga caccgacgta cgaagagatc aacgccgcgc
     7921 tcaagaagcc ggaggagttc cgttacatcg gcaacggcaa ggaaaagatg ccgttcgtcg
     7981 acgcgtcgga catggtgcag ggaaaggcag tctacggagc tgatgtcgac
//
LOCUS       DATLVW010000363         4020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_425650, whole genome shotgun sequence.
ACCESSION   DATLVW010000363 DATLVW010000000
VERSION     DATLVW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425650"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..2076
                     /locus_tag="VNN25_11025"
     CDS             304..2076
                     /locus_tag="VNN25_11025"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HXH92103.1"
                     /translation="MSRVRTLVIAVLGFLASMCASVPQHHPAAIPPIEQLSLDDKVGQ
                     LFAVIGHGVFMSESSMAYRELLHHVRDNHAGGVMWSVSNVYETAQLTARLQSASKIPL
                     LISADLESGMGMRFLDTTYWPWAMAVAATGDPSLAEQEGRVVAREAKMVGINHIYAPV
                     ADVNVDPDNPVINTRSFGEDPADVSRFVAAFVRGVQSEHVLATAKHFPGHGDTHVDSH
                     RSLPILAVDRSRLEKVELLPFRAAIDAGVKSIMIGHLAVPALDEELVPVRPPDPGENP
                     YGTKASEVPQNGTMPATTSKKMITGLLRHDLKFDGLVVSDAMDMGGITEHFSAGEAAI
                     RAIEAGQDQILLSPNVDAAVAAVKAAVVSGRLPESRIDESVRRILAAKAFAGAGVGTP
                     DEIFAGIDTKEHRELAQSIARRAVTLVREQARALPVRKSARVVIITVSDFQELVSPLP
                     DFEREMRRRLTAPPQTFLIDARTTPENIAPIAAAAQNADAVIFAMAVRTRSGSGSIAT
                     PESARQLLADLAPRIPTFAISFGTPYLLREIPTTDTYICAYGIQPVMQVAAVHALFGE
                     APVSGKLPVTIPGLAMRGAGIMKP"
     gene            2073..3890
                     /locus_tag="VNN25_11030"
     CDS             2073..3890
                     /locus_tag="VNN25_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXH92104.1"
                     /translation="MSTFALRPLDYAIVAAYLSLIVAMGIYFGRKAKRSMLDYFLSHR
                     SMPWWLAGTAMVATTFSADTPLAVTEMVVKNGVAGNWLWWSMLGSGMLTVFFFARLWR
                     RAEVVTDVELTELRYSGRPAAFLRGFRAAYLGLIANIIVMGWVNLGMAKVLQGTLGIS
                     NRSALFVCLIVVFAYTVYSGYWGVATTHGVQYLFEMGGAITLAVVALSAVGGVDVLKA
                     KLAFAHPAGTPIGTTFGTSAAAMSFWPTGDGLWVVPTITLAALLGLNWWASWYPGSEP
                     GGGGYVVQNMLACRTEREARSAALFFNIAHYALRSWPWVITALCTLVMYGGAVKEGDP
                     GINYVRAMVDLLPTGLKGLMLASFVAAFSSTQATQMNWGSSYLVNDLYRRFIKRDASE
                     KHYVLASRLATALTLVLSLIATQFMDQVSHAWQFLLMLGAGTGLVYILRWYWWRVNAW
                     SEVSAMAAALIVSLSIRNFIDSSSPNGFAIGLIITTAATTLVWLVVTMLTQPEPRATL
                     DAFYKKVRPAGPGWGVIARESGIEPVRGELGRNAIAWVAGIVMVYSIMFATGAAIFHN
                     ARQFAIFGSALVASAVLLVVIMRRERAETDPQMTQMEHR"
BASE COUNT          674 a         1304 c         1274 g          768 t
ORIGIN      
        1 gcgtatctac gcccccctca tccggcttcg ccaccttctc cccccgcaaa aagcgcgggg
       61 ggagaaggcg ctcgatggga aagcactcca aagaaatagg acgaggtcta gtaaggagat
      121 gcagcatgca ctaagcgtgt gagaaatgcg ctacgccggc ggcacattct tcgtcgcgct
      181 ccaccgccct tccgccaccg tggttccgcc gattcagaat gacatccgcg cgctagcgtc
      241 gcttcatcta ccacccccga ccccctactc cccgcacccc acatcccgta tccttcccca
      301 cgcttgtctc gcgtccgaac cctcgtcatc gccgtgctag gcttcctggc ctcgatgtgt
      361 gcgtcggtcc cgcaacatca ccccgctgcg attcctccga tcgaacaact ctcgctcgat
      421 gacaaggtcg ggcagttgtt tgcggtgatc ggacatggtg tcttcatgtc cgaatcgtcg
      481 atggcctatc gggagttgct gcatcacgtt cgcgacaatc atgccggcgg cgtgatgtgg
      541 tcggtatcga atgtctacga gaccgcgcag ttgaccgcgc gtctgcagag tgcgtcaaag
      601 atcccgctgc tgatcagcgc cgacctcgag tcgggtatgg gcatgcgttt tctcgacacg
      661 acctactggc cctgggcgat ggccgtggct gcgaccggcg atccgtcgct ggccgagcag
      721 gaagggcggg tggtggcgcg ggaagcgaag atggtcggga tcaaccacat ctacgcgccg
      781 gtcgccgacg tcaacgtcga tcccgacaat cccgtgatca acacgcgcag cttcggcgaa
      841 gatccggccg acgtcagccg tttcgtggcg gcgttcgtgc gcggcgtgca gtcggagcat
      901 gtgctggcga ccgccaaaca ctttccggga catggcgaca cgcatgtcga ctcgcatcgc
      961 tcgctgccga tccttgccgt cgatcgttcc aggctggaga aggtcgagct gctgcccttt
     1021 cgcgccgcca tcgacgccgg tgtgaagtcg atcatgatcg gacatctcgc ggtgcctgct
     1081 ctcgatgagg agctcgtgcc ggtgcggcca cccgatcctg gcgagaaccc ctacggaacc
     1141 aaggcgtcgg aagtgccgca gaacgggacg atgcctgcga cgacgtcgaa gaagatgatc
     1201 accggcctgc tgcgccacga cctcaagttc gacggactcg tcgtcagcga tgccatggac
     1261 atgggtggca tcaccgagca cttcagcgcc ggcgaagcgg cgatccgcgc catcgaagcg
     1321 ggtcaggatc agatactgct gtcgccgaac gtcgatgccg ccgttgccgc ggtgaaggcg
     1381 gcggtcgtga gcggccggct gccggagtcg cggatcgacg agtcggtgcg gcgcattctc
     1441 gctgccaaag cattcgctgg cgccggcgtg ggaacgcctg acgagatctt tgccggcatc
     1501 gacacgaaag aacaccgcga gcttgcgcag agcatcgcgc ggcgcgcggt cacgctcgtg
     1561 cgcgaacagg cccgagcgct gccggtgagg aagagcgcac gcgtcgtcat catcaccgta
     1621 agcgattttc aggaactggt atcgccgctg cccgacttcg agcgcgagat gcggcgcagg
     1681 ctgaccgcac cgccgcagac gttcctgatc gacgcgcgca cgacgcccga gaacatcgct
     1741 ccgatcgccg ccgcggcgca gaacgcggat gcggtcatct ttgcgatggc ggtgcgcacg
     1801 cgctccggtt cgggatcgat cgcaacgccc gaatcggcgc gccagctctt ggccgatctc
     1861 gcgccgcgga tcccgacctt cgccatctcc ttcggcacgc cgtacctgct gcgcgagatc
     1921 ccaaccaccg atacatacat ttgcgcgtac gggatccagc cggtcatgca ggttgcggca
     1981 gtgcacgcgc tgttcggcga ggcgccggtg tccggcaagc tgccggtcac gattccggga
     2041 ttggcgatgc gaggagcggg gatcatgaag ccatgagcac gtttgcgctg cggccgctcg
     2101 actacgcgat cgtcgctgcc tatctgtcgc tgatcgtcgc gatggggatc tatttcgggc
     2161 gcaaggccaa gcgctcgatg ctcgactact tcctctcgca ccgatcgatg ccctggtggt
     2221 tggcgggcac ggcgatggtg gcgacgacgt tctccgccga cacgccgctg gccgtgaccg
     2281 agatggtcgt caagaacggc gtggccggga actggctgtg gtggtcaatg ctcggctccg
     2341 gcatgctcac agtcttcttc ttcgcgcgtt tgtggcgccg cgccgaagtg gtgacggacg
     2401 tcgagctgac cgagcttcgc tactcgggcc gtccggccgc cttcctccgc ggtttccgcg
     2461 ccgcctacct cggcttgatc gccaacatca tcgtcatggg ttgggtcaac ctcggcatgg
     2521 ccaaagtgct tcagggcacg ctcggcatct ccaaccgcag cgccctcttc gtctgcctca
     2581 tcgtcgtatt tgcctacacc gtctactcgg gttactgggg tgtcgccacg acgcacggcg
     2641 tgcagtacct cttcgaaatg ggaggggcga tcaccctggc cgtcgtcgcg ctcagcgcgg
     2701 tcggcggcgt cgatgtgctg aaagcgaagc tggcgttcgc gcatccggcc gggacgccga
     2761 ttggcacgac tttcgggact tccgcggctg cgatgtcgtt ctggccgacc ggcgacggat
     2821 tgtgggtcgt gccgacgatc acgctcgccg cgctcctcgg cctgaactgg tgggcgtcgt
     2881 ggtatcccgg ctccgagcca ggcggcggcg gctatgtcgt gcagaacatg ctggcctgcc
     2941 gcaccgagcg cgaagcgcgc agcgcagcgc tcttcttcaa catcgcccac tacgcgctgc
     3001 ggagctggcc gtgggtgatc accgcgctct gcacgctcgt gatgtacggc ggcgcggtaa
     3061 aggaaggcga tccggggatc aactacgtgc gggccatggt cgatcttctg ccgaccggcc
     3121 tgaagggtct gatgctggcc tcctttgtcg ctgccttcag ctcgacgcag gcgacgcaga
     3181 tgaattgggg ctcgagctat ctcgtaaacg atctctaccg ccgtttcatc aagcgcgacg
     3241 ccagcgagaa gcactacgtt ctggcgtcac gtctggcaac agcgctcacg ctggttctct
     3301 cgctcatcgc gacgcaattc atggatcagg tcagtcacgc ctggcagttc ctgctgatgc
     3361 tcggcgcagg aacgggcctc gtctacatcc tgcgctggta ctggtggcgc gtgaatgcct
     3421 ggtcggaagt gagcgcgatg gcggcggcgc tcatcgtttc tctcagcatc cgcaacttca
     3481 tcgactcttc ctcgccgaac ggcttcgcga tcggcctcat cattacgaca gcagcgacga
     3541 cgctggtctg gctcgtcgtg acgatgctga cccagccgga gccgcgcgcg acgctcgatg
     3601 cgttctacaa gaaagtgcgc cctgccggcc cgggatgggg cgtgatcgcg cgtgaaagcg
     3661 gcatcgagcc ggtgcgcggc gagcttggcc gcaacgccat cgcctgggta gccggcattg
     3721 tgatggtcta ctcgatcatg ttcgcgactg gcgcggcgat cttccacaac gcacggcaat
     3781 ttgcgatctt tggctcagcg ctggtcgcct ctgcagttct cttggttgtg atcatgcgta
     3841 gggaacgagc ggagaccgat ccgcagatga cgcagatgga acacagataa aaacgttctt
     3901 cataatctgc gtctcatctg cggatgaggt ttcagccgaa gagctccgca agcgagagcc
     3961 gccatccagg aatgacgtcg tcgatctcga tcacatcctc gaccttggta gcgctgtccg
//
LOCUS       DATLVW010000364         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_223701, whole genome shotgun sequence.
ACCESSION   DATLVW010000364 DATLVW010000000
VERSION     DATLVW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223701"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1827)
                     /locus_tag="VNN25_11035"
     CDS             complement(160..1827)
                     /locus_tag="VNN25_11035"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXH92105.1"
                     /translation="MRRLLALAVLLLAACATTPKTDYDIVLRHATLYDGSGKPATQGD
                     LAIRADRIAAIGDVPGRGAKEIDVHGLAVAPGFINIMSQAQESLIADGRGLSDTKQGV
                     TTEIFGEGESMGPLSPAMKKEFVDSEADIRYPIEWTTLREYLDWLQHRGVTPNIGSFI
                     GAATPRINVLGRENRDPSPAELQQMQDITRKAMDDGAFGVASALIYAPGTFAKTPELI
                     SLAKVAGERGGIYISHMRSEGNRLIEAVDELITIAREAHVPAEIYHLKAAGQANWPKM
                     DELIRHVDAARAAGLQVSADMYTYTAGMTGLDAAMPTWVQAGGYEEWRKRLQDPATRQ
                     RVIAEMRTQSDTWENLMMLAGGADRVLLVGFKSDALKPLTGKTLAEVAKMRGVSPEDA
                     AIDLVIEDGTRVSTIYFLMSEDNLRKQIALPYVSFGSDADSSAPEGVFLKSSNHPRAY
                     GNFANLLGKYVRDEHVILLAEAVRRLTSLPAQTLGIANRGLLAPGNFADVVVFDPNTI
                     GAQSTYEKPMQFATGVVDVFINGTQVLQNGEHTGTKAGRVVVRERPR"
BASE COUNT          341 a          660 c          702 g          395 t
ORIGIN      
        1 cctaatttcg ccagccggaa tggtaatttc atctactcca tggataggtt cgggtgtcag
       61 atcgctggcc tataagtatc catggacatc taattggtgt catgacccct tcaaaaaccc
      121 gctgatccac agatgagcgc agatggacgc agataaaacc tacctcggcc gctcccgcac
      181 caccaccctc cccgcttttg tgccggtgtg ctcgccgttt tgcagcacct gggtgccgtt
      241 gatgaagacg tcgaccacgc cggttgcgaa ctgcatcggc ttttcgtagg tggattgggc
      301 gccgatcgtg ttgggatcga agacgacgac atcggcgaag ttgccggggg cgagcaggcc
      361 gcggttggcg atgccgaggg tttgggctgg gagggaggtc aggcggcgca cggcctcggc
      421 cagcaggatc acgtgctcgt cgcggacgta cttgccgagg aggttcgcga agttgccgta
      481 ggcgcggggg tggttcgacg acttgaggaa gactccctcg ggagcggagg agtcggcgtc
      541 ggagccgaac gagacgtacg gcagcgcgat ctgtttgcgc aagttgtcct cggacatcag
      601 gaagtagatc gtgctgacgc gggtaccgtc ttcgatgacg aggtcgatgg ccgcatcctc
      661 gggggacacg ccgcgcatct tcgccacctc ggccagcgtc ttgccggtga gcggcttgag
      721 cgcgtcgctc ttgaagccga ccagcagcac gcggtcggcg ccgccggcca gcatcatcag
      781 gttctcccac gtgtcgctct gcgtgcgcat ctccgcgatc acgcgctggc gtgtggccgg
      841 atcctgcaac cgcttgcgcc actcttcgta gccgcccgcc tgcacccacg tcggcatcgc
      901 cgcatcgagg ccggtcattc cggcggtgta ggtgtacata tcggcggaga cttgcagccc
      961 cgcggcgcgc gcggcatcga cgtgccggat cagctcatcc atcttcggcc agttcgcctg
     1021 accggccgct ttgaggtggt agatctcggc gggaacgtgc gcctcgcgcg cgatcgtgat
     1081 cagttcatcg acggcttcga tgaggcggtt cccctcgctg cgcatgtgcg agatgtagat
     1141 gccgccccgc tcaccggcga ccttcgccag cgagatcagc tccggggtct tcgcgaacgt
     1201 tcccggcgcg taaatgagcg ccgaggcgac gccgaaggcg ccgtcgtcca tggctttgcg
     1261 cgtgatgtcc tgcatctgct gcaactcggc cggcgatgga tcgcggttct cgcggccgag
     1321 gacgttgatg cgcggcgtgg cggccccgat gaacgagccg atgttcggcg tgacgccgcg
     1381 atgctggagc cagtcgaggt actcgcgcag cgtcgtccat tcgatcggat acctgatgtc
     1441 ggcctcggag tcgacgaact ctttcttcat cgccggcgag agcggtccca tcgactcgcc
     1501 ttcgccgaat atctcagtgg tgactccctg cttggtgtcg gagagcccgc ggccgtcagc
     1561 gatgagcgac tcctgggcct gactcatgat gttgatgaat cccggcgcca cagcgaggcc
     1621 gtgaacgtca atctccttcg cgccgcggcc cggcacgtcg ccgattgcgg cgatgcgatc
     1681 ggcgcggatg gccaggtcgc cctgcgtcgc aggcttgccg cttccgtcgt agagcgtggc
     1741 gtggcggaga acgatgtcgt agtcggtctt cggcgtcgtg gcgcaggcgg cgaggaggag
     1801 gacggcaagt gcgagaaggc gtctcatgtc gcgcacgagc gtaacgcagt ctcacgcgaa
     1861 gccgcgaagg tcgcgaagaa gagcatcgta aacatcagcc cttcgcgtct tcgcgtcttc
     1921 gcgtgagaaa gtcgttgcgc cgcgcacaga gaccgtgagt cgggcagcca tcccgccccc
     1981 aagctctcgg gcgggacgcc cgagagcccg cgggcgaaga cgcccgcact ccttacttaa
     2041 tcacgaccgg cggcgtcgag acgtttgtca cggtcaccgt gccgttgcca ctcgagac
//
LOCUS       DATLVW010000365         5484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_97359, whole genome shotgun sequence.
ACCESSION   DATLVW010000365 DATLVW010000000
VERSION     DATLVW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97359"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..674)
                     /locus_tag="VNN25_11040"
     CDS             complement(129..674)
                     /locus_tag="VNN25_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92106.1"
                     /translation="MASMFSALLFLVATAAAPSRLPNACQLLSAREIAHVQGEAFRSA
                     KLTETEADGLRVSQCFYALPSFTNSVSVDLMHGKTSAFWKAHFSGARDERGADEDHDR
                     DAAMKTAPPSREAEEEHQSAVRKVSGVGDTAVWSGNRMSGALYVLKGDAIVRISVGGK
                     GSEDEKIERSRKLAVVALRKL"
     gene            complement(682..4140)
                     /locus_tag="VNN25_11045"
     CDS             complement(682..4140)
                     /locus_tag="VNN25_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92107.1"
                     /translation="MSKRPRLIVVALIALAAFVLSSNLFAQTTYYFKGDPADQANKVA
                     NDTASGTIGTATFDTSPPTGTAPVIQTGPPSVANADYVGNPLAIYWSGQYSGTLAGTL
                     DFKWYWSTGNPETIGVGGAIEVSVFADPDYTADRAQPQRLIGRAIVPLTGISATPTLI
                     ESLVPVNGSVSSTLLIQVIPQFVDSGNGLAALYNSTTTPSSFVFTTAQRTPFPAATPA
                     TGQPPRFSLFTPSAAQLATGLGTDAGEPSIGSNWITGNVLFQSFVTTFRVSFDDSCPT
                     NPTSTWVAKQSQITGNESFDPILWTDSKVGRTIVSQLVFASTESLSAVTDNDGDLWLP
                     SQGAGIASGIDHQTVGGGPFHQPLLAGAFYPNAVYYCAQDLALANCALSLDGGLTYGP
                     AVPIYTSQCGGLHGHVKVGADGVAYVPNKDCGGKQGVVVSEDNGVTWNVRTVPNSLAA
                     GSDPSVGVSKNGRVYLGFADNNNSPVIAISDDHGQSWHDVKDVGAAAGIKNTVFSEVV
                     AGDDNRASFAFLGSNSIGALDARTYPGVWYMYVATTYDGGSTWHLVNATPNDPVQRGP
                     VWLSGGAEISRNLLDFNDMTIDKQGRMLIGFADGCVDACVQGPATARGNSYTAVASIL
                     RQTGGRRMFAEFDPAEPTAPSAPALTVTRNGSLARLSWSEGNNGGSAITNYSVRRDGT
                     AIASTTATSYTDPTANPSQTYSYSVVATNSVGSSCGSNAVSSAGGGGSSCTLPGVTVM
                     TDPTGDGAAATNASLDIQSVSIAEPYYSDGSQKLVFTMKVASLASVPPGAEWRILWNF
                     PTTDTGSYYVEMNTDPTGVVAYQYGTIDLTSAVVTAVGQPHLLGTCDAESTFLPDGTI
                     RLVLSTSKIGNAAAGDIIGGLLARTFVTSGSVVTSSRSAIDTASTTGTYLMVGNDFCA
                     PPSLTTIEDDDARIAYSNGWHLLSDGNASAGHFRMGSGKATASLTFSVPSNKFGSITF
                     NYAKSTKGGTAEMFLDGVSQGTIDFSGPLGTLKNPQFTFSKTYGPLQPGSHTLQLRGN
                     GTTYLDNFVLQSSSSNAQPTSGPGTTSTSTSNLSLGQELVSQITLPDNAQAISIVAES
                     SNGLLLRVGLVDATGLSLATADTSSGLAVINQPLTHGGVYLIKTVNLSAGPVSVWTAT
                     TPLLSR"
     gene            complement(4311..5444)
                     /locus_tag="VNN25_11050"
     CDS             complement(4311..5444)
                     /locus_tag="VNN25_11050"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXH92108.1"
                     /translation="MNHRFLIAAAVLLTAIGASAQSALTLPPSGGNERATVIQHIGLV
                     TVSIDYSSPHVHSPQGQDRRGKIWGTLVPWGLTNLGFGTCKECPWRVGANVNTIFTTS
                     HDIQVNGSTLPAGSYALFAIPQQDEWTWIFSKNHTSWGSFFYDPAEDALRVKAKPEKS
                     DYHEVLTFDFTERRNDHATVAMKWEELAVPIDINVPNAADLYIANLRNELRDSPGFND
                     TTWQQAARYALDHKHPAEALAWAETANNGGGGIGRKNFTNLMTLAEAYEANGKTAEAA
                     KAREEALSHPTATALDLHAYARALLAGGKKAEAIQVWELNAKRFPGMWPVNVGLARGN
                     SAAGRYPEALKYAKLALAQAPDDQNKKSLEGMVKKLEAGQDIN"
BASE COUNT          926 a         1597 c         1857 g         1104 t
ORIGIN      
        1 tgtgtcacag agatcccctc tgtgttctca gtgtctctgt ggttgccttt gcttcggcga
       61 ccacgataag cgtggcaaat tgacccatta cgtgtgaggc cggaagcagc cggcctcacg
      121 gttagtgtct acagtttacg cagcgccacg acggcgagct ttctcgatcg ctcgatcttc
      181 tcgtcctcgc tgcctttgcc gccgacgctg attcggacga tagcgtctcc tttgaggacg
      241 tagagcgcgc ccgacatccg gttgccgctc cagacggcgg tgtcgccgac gccggacact
      301 ttgcgcacgg cgctttgatg ttcttcctct gcctcgcgcg atggcggagc ggtcttcatt
      361 gcggcgtcgc ggtcgtgatc ttcatcggcc ccgcgttcgt cgcgtgcgcc tgaaaaatgc
      421 gctttccaga aggcgctcgt cttgccgtgc atcagatcga cgctgaccga gttggtgaac
      481 gacggcagcg cataaaagca ttgcgacacg cgcaacccat cggcctcggt ctccgtgagc
      541 ttggccgatc ggaacgcttc gccttgcacg tgcgcgatct ctcgcgccga cagcaactgg
      601 caggcattgg gcaaccgcga cggcgccgcc gcggttgcca cgaggaaaag aagtgcagag
      661 aacatgctgg ccatttgatt gttatcggct caggagtggt gtcgtcgccg tccagacgct
      721 cactggcccg gcgctgaggt tgacggtctt gatgaggtag acgccgccgt gcgtcagggg
      781 ctggttgatg acggcgaggc ctgatgacgt gtccgcagtg gccagcgaaa ggcctgttgc
      841 atcgacgagt ccgactcgca gcagcaatcc attcgacgac tcggcgacga tggagatcgc
      901 ctgcgcgttg tccggaagcg tgatctgcga taccagctcc tggccaagcg acagattgga
      961 cgtgctcgtg gaagtcgttc ccggtccgct cgtaggctgc gcattcgacg acgagctttg
     1021 gagaacgaag ttgtcgagat acgtggtgcc attaccgcgg agctgcagcg tgtgcgagcc
     1081 cggctgcagt ggtccgtagg tcttgctgaa tgtgaactgc gggttcttca gagtgccgag
     1141 cgggcctgag aagtcgatcg tgccctgcga gacgccgtcg aggaacatct cggcggtgcc
     1201 gcccttcgtg ctcttcgcgt agttgaacgt gatcgagccg aacttgttcg atggcacgga
     1261 gaacgtcagg cttgcggtcg ctttgccgct gcccatgcgg aagtggccgg cgctggcgtt
     1321 tccatcgctc agcagatgcc agccgttcga atacgcgatg cgtgcgtcgt cgtcttcgat
     1381 cgtcgtcagc gacggcggcg cgcagaagtc gtttccaacc atcaggtagg tgccggtcgt
     1441 cgacgcggtg tcgatcgccg agcggctgga ggtgacgacg cttccggatg tgacgaatgt
     1501 acgagcgaga agaccgccga tgatgtcgcc ggcggcggca ttgccgatct tcgatgtgga
     1561 gaggacgagc cggatcgtgc catcgggcag gaacgtcgac tccgcgtcgc aggtgccgag
     1621 gaggtgcggc tggccgacgg cggtgacgac cgcgctggtc aggtcgatcg tgccgtactg
     1681 gtaggcgacg acgccggtcg gatcggtgtt catctcgacg tagtacgagc cggtgtcggt
     1741 ggtcgggaag ttccagagga tgcgccattc ggcacccgga ggcacgcttg caagcgaagc
     1801 gaccttcatc gtgaagacga gcttctgcga gccgtcgctg tagtacggct cggcgatgga
     1861 gaccgactga atgtcgagcg aggcatttgt ggctgcagcg ccgtcgccgg tcggatcggt
     1921 catgaccgtg acgcccggta gcgtgcagga gcttccgccg ccgcctgccg agctgacggc
     1981 gttgctgccg cacgacgagc cgacggagtt cgttgcaacg acggagtagc tgtaggtctg
     2041 cgaaggattg gcggtcggat cggtgtacga agtggcggtg gtgctggcga tggccgtgcc
     2101 gtcgcgtcgc acggagtaat tcgtgatcgc ggaaccgccg ttgttccctt cggaccacga
     2161 cagtctggcc agcgatccgt tgcgcgtgac cgtgagagcg ggggcagagg gagcggtggg
     2221 ttcggccggg tcgaactcag cgaacatccg ccgtccaccc gtttgacgca ggatcgaggc
     2281 cactgccgtg tacgagttgc cacgcgcggt tgccggcccc tgaacgcagg cgtcgacgca
     2341 accgtcggcg aatccgatga gcatgcgtcc ctgtttgtcg atggtcatgt cgttgaagtc
     2401 gagcagattc cgcgagatct ccgcgccgcc gctgagccag accgggccgc gctgcacggg
     2461 atcgttggga gtggcgttga cgaggtgcca tgtggatccg ccgtcgtagg tcgtggcgac
     2521 gtacatgtac cagacgcccg gataggtgcg cgcgtcgagc gcgccgatac tgtttgaccc
     2581 gaggaacgcg aaggaggcgc ggttgtcatc gccggccacg acttccgaga agaccgtgtt
     2641 cttgatgccg gccgctgcgc caacgtcttt cacatcgtgc cagctctgcc cgtgatcgtc
     2701 ggagatggcg atgacaggtg aattgttgtt gtcagcaaag ccgaggtaga cgcggccgtt
     2761 cttcgaaacg ccgaccgacg gatcgctgcc cgcggcgagg ctgttgggca ccgtcctcac
     2821 gttccacgtg acgccgttgt cttcggagac gacgacgccc tgcttgccgc cgcagtcttt
     2881 gttcggtacg taggcgacgc cgtcggcgcc gactttgacg tgaccgtgca acccgccgca
     2941 ctgcgaggtg tagatcggga ctgccggtcc gtaggtcagg ccgccgtcga gcgacagcgc
     3001 gcagttcgca agggccaggt cctgggcgca gtagtaaacg gcattcggat agaacgcgcc
     3061 tgcaagcagc ggctgatgga acggtccgcc gccgaccgtc tggtgatcga ttcccgacgc
     3121 gattccggcg ccttgcgacg gcagccagag atcgccgtcg ttgtcggtaa cggcggagag
     3181 actctccgtg ctggcgaaga ccaattgcga gacgatcgtc cggcctacct tcgagtccgt
     3241 ccagagaatc ggatcgaagc tctcattgcc ggtgatctgc gattgtttgg caacccacgt
     3301 cgaggtggga ttggtcgggc aggagtcatc gaacgagacg cggaaggtgg tgacgaacga
     3361 ttggaagaga acgttgccgg tgatccagtt cgagccgatc gaaggctcgc cggcgtcggt
     3421 gccgagtccg gtggcgagct gcgccgccga cggcgtgaag agactgaatc gcggcggctg
     3481 tccggtagcc ggcgtggctg ccgggaacgg cgtgcgctgc gcggtagtga agacgaaact
     3541 cgatggcgtc gtcgtcgagt tgtaaagcgc ggccagtcca tttcccgagt cgacgaactg
     3601 cgggattacc tggatgagca gcgtgctcga taccgatccg ttgacgggaa cgagactctc
     3661 gatgagagtc ggcgtcgcgc tgatcccggt cagaggaacg atggcgcggc cgatgagccg
     3721 ttgcggttgc gcgcggtccg cggtgtagtc gggatcggcg aacacgctga cctcgatcgc
     3781 gccaccgact ccgatcgttt ccggattgcc ggtcgaccaa taccatttga agtcgagcgt
     3841 gccggcaaga gtccccgagt actggccgga ccagtagatg gccagagggt tgccgacgta
     3901 gtccgcgttg gcgacggaag gcgggccagt ctggatcacc ggcgctgtgc cggttggcgg
     3961 gctggtatcg aaggtcgcgg taccgatcgt gccgctggcg gtgtcgttgg cgactttgtt
     4021 ggcctgatcg gcggggtcac ctttgaaata gtaggttgtt tgagcgaaaa ggttggagga
     4081 cagaacgaag gcggcgaggg cgattagggc gactacgatc aatcgagggc gcttcgacat
     4141 gaacaactct cccattctgc gttatttacg ggcccttagt agtagtgcta ccaacatgcg
     4201 gcgacggtat tgctactttc gcgcctactt ttcagggagt gccgagggga attgctaata
     4261 aaaaggcccg ccggaaccgg cgggccttgt caaagctccg aaaatggaga ctagttgatg
     4321 tcctgacctg cctcgagctt tttcaccatc ccttcgaggc tcttcttgtt ctggtcgtcg
     4381 ggcgcctggg ccagggccag ctttgcgtac ttcaaggctt ccggatagcg ccccgcggcc
     4441 gagttgccgc gagcaagacc cacattcacc ggccacatgc ccggaaagcg cttggcattc
     4501 agttcccaca cctggatcgc ttctgctttc tttcctccgg ccaggagtgc tcgcgcgtag
     4561 gcgtgcagat cgagcgccgt cgcagtggga tggctcaacg cctcctcccg cgctttcgcc
     4621 gcctccgccg tcttgccgtt tgcctcgtag gcctcggcaa gcgtcatcag gttcgtgaag
     4681 tttttccggc cgatgccgcc tccgccgttg ttggccgtct cggcccacgc cagcgcttcg
     4741 gccggatgtt tgtggtcgag cgcatacctc gccgcctgct gccacgtcgt atcgttaaat
     4801 cccggcgagt cgcggagctc attgcgaagg ttggcgatat agagatcggc ggcattcggc
     4861 acgttgatgt cgatcggtac ggccagctct tcccacttca tcgcgacggt tgcatggtcg
     4921 ttgcgccgtt cggtgaagtc gaaggtcagg acctcgtgat agtcactctt ctccggtttc
     4981 gccttcacgc gaagcgcgtc ctcggcaggg tcatagaaga agctgcccca cgatgtgtga
     5041 ttcttcgaga agatccaggt ccactcatcc tgctgcggaa tggcgaagag cgcgtacgag
     5101 ccggcgggca gggtcgagcc gttgacctgg atgtcgtgcg aagtcgtgaa gatggtgttc
     5161 acgttggcgc cgacgcgcca ggggcattcc ttgcaggtgc cgaatccgag gtttgtcagg
     5221 ccccacggca cgagcgtccc ccagatcttg ccgcggcggt cctgcccctg cgggctgtga
     5281 acgtgcggac tgctgtagtc gatcgagacc gtgacgagac cgatgtgctg gatcacggtt
     5341 gcgcgttcgt tgccgcccga gggaggaaga gtgagtgccg actgcgcgct ggcgccgatg
     5401 gccgtcagca gaacagccgc tgcgatgaga aaccgatggt tcatagaccg ctcctttcga
     5461 atgcgtcaat gtacgtacgg ttct
//
LOCUS       DATLVW010000366         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_264749, whole genome shotgun sequence.
ACCESSION   DATLVW010000366 DATLVW010000000
VERSION     DATLVW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264749"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VNN25_11055"
     CDS             complement(<1..129)
                     /locus_tag="VNN25_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH92109.1"
                     /translation="MKLTHITKRFGTNVAVDDVSLAFAPGEIVGLVGENGAGKTTLM"
     gene            complement(228..1196)
                     /locus_tag="VNN25_11060"
     CDS             complement(228..1196)
                     /locus_tag="VNN25_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HXH92110.1"
                     /translation="MKRILALASLAFLLLGCSKPEGSSAMKVGLLTPGSVNDNGWNAI
                     AYEGLQEIHKQLHAEISNQETKTPAEFEEGFRSYGEKGYDLAFGHGFEFQDAALKAGK
                     QYPKTIFITTSGSSVAPNVSPMVFELEQATYLLGVIAGRESKTGKAGVVGGINLPSIA
                     STFLAFRAGAQSVNPNFEVKEIYTGNFDDPGAAKLATLSLINAGCDFIFHQANEAGRG
                     VFQACQERKVRCFGSNKNQNDMAPDVIVASAVLDVPGAFVYMANLVHDHKFKPQIYWL
                     GRRQRVVSLVWNDRLKSSLSPQTVAEVEKLDAAIQAGTFTVPRGKF"
     gene            1425..1616
                     /locus_tag="VNN25_11065"
     CDS             1425..1616
                     /locus_tag="VNN25_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92111.1"
                     /translation="MDDIERDGNEGDDDEQFEQMIVENSILLHSLAALLVRKGLIKQD
                     EIDAEMDKLYDEMEKFDGE"
     gene            1689..>2862
                     /locus_tag="VNN25_11070"
     CDS             1689..>2862
                     /locus_tag="VNN25_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXH92112.1"
                     /translation="MKIRNLLMAAAVLAATATSGFAALSPAKAEWGKGPVQYIMTNEE
                     KAAWKALQTDADADAFIDLFWARRDPTPTTPRNEFREQFEASVKSADNQFGHGRKKGS
                     MTDPGRVLIVFGPPTAAKRTSKIGEANTRQNMRDIEIGSAMSGSGSADMTWTYEAALA
                     QKAFGLPRLDVAFHDQYGEGDFKLTTPLVDANSAMTQKMIASFITQPGLTKAPTFQAA
                     GAAPVVPPPPAAAATTLKTAALEAAVVEAKGGKGNNHGAALAYAELISPAGEPFVPVS
                     VYVPASAGVAADGADTIFGAVEDASGKRVTMFEEPAKLTASRTDFFVDKTLTLEPGKY
                     TAVVGLAKAGQPVLVTTGPIEVTGPSKEAVGTSRLILSDNVYELGTAEPPKSPFAFG"
BASE COUNT          572 a          862 c          908 g          520 t
ORIGIN      
        1 catcagcgtc gtctttcccg cgccgttctc cccgacgagt ccaacgatct cgccgggcgc
       61 gaacgcgagc gaaacgtcat cgacggcgac gttggtgccg aagcgcttgg tgatgtgcgt
      121 cagcttcatg gggtcagacc caaagtgaaa gtgacgaaaa acgtccttcg ttcatgaggc
      181 aagccatttc tcgtcacttt cactttgggt ctgacccctg gctcgagtta gaacttaccc
      241 cgcggaaccg taaacgtccc cgcctggatt gccgcgtcga gtttctccac ctcggcgacg
      301 gtctgcggcg acagcgacga tttgagccgg tcgttccaga ccagcgagac gacgcgctgc
      361 cggcgcccga gccagtagat ctgcggcttg aatttgtgat cgtggacgag gttcgccatg
      421 tagacgaacg cgccgggcac gtcgaggacc gccgaggcga cgatgacgtc gggcgccata
      481 tcgttctggt tcttgttcga tccgaagcag cggaccttgc gctcctgaca ggcctggaag
      541 acgccacggc cggcctcgtt ggcctgatgg aagatgaagt cgcagccggc gttgatcagc
      601 gagagcgtgg ccagcttggc cgcgccgggg tcatcgaagt tgccggtgta gatttccttc
      661 acttcgaaat tcgggttcac cgactgtgcg ccggcccgga aggccaggaa ggtcgacgcg
      721 atcgatggca ggttgatgcc gccgacgact ccggcctttc ccgttttcga ctcgcggcct
      781 gcgatcacgc cgaggaggta ggtggcctgc tcgagctcga agaccatcgg agagacgttc
      841 ggcgccaccg agctgcctga cgtcgtgatg aagatcgtct tcgggtactg cttgccggct
      901 ttgagcgcgg catcctgaaa ttcgaagccg tgaccgaagg cgaggtcgta acctttctcg
      961 ccgtacgatc ggaacccttc ctcgaactcg gcaggtgtct tcgtctcctg gttggagatt
     1021 tccgcgtgga gctgtttgtg aatctcctga agcccttcgt aggcgatcgc gttccatcca
     1081 ttgtcgttga cggaaccggg agtgagcagt cctaccttca tggccgatga gccctcgggt
     1141 ttcgagcagc cgaggagaag gaaggcgagg gaggcgagtg cgaggatgcg tttcatggcg
     1201 cggattgtag cttctgcgct tcgcgcgttc ccggttgtcg gttggttgcg cttcgcgcgt
     1261 taactgccga gtacgggctg gcggcgcaag tggtcgaagg gggcgcaacc cgtcacaggc
     1321 ccccgtgaca actacgcggc gaagccgcaa tcaaccgaca accgacaacg cgcgcagcgc
     1381 aatttgtgat acatcctcac cagcgcggaa ggaggcgcaa cctcatggac gacatcgaac
     1441 gggatggcaa tgaaggcgat gacgacgaac agttcgagca gatgatcgtg gagaacagca
     1501 tcctgcttca cagcctcgct gcgctgctgg tccgcaaggg gctaatcaag caggacgaga
     1561 tcgacgcgga gatggacaaa ctctacgacg agatggagaa attcgacggc gaatgacggc
     1621 accgttcgaa tggaataggc ccgatcctga gatgtttcgg acgaacgtcg attcaaccgg
     1681 agaaactgat gaaaattcgt aacttgttga tggccgctgc cgttctggca gcaacggcca
     1741 ccagtggttt tgccgcgctc tcgcccgcca aagcggagtg gggcaagggc ccggtgcagt
     1801 acatcatgac gaacgaagag aaggcggcgt ggaaggcact gcagacggac gccgatgccg
     1861 acgccttcat cgaccttttc tgggctcgcc gcgatcccac gcccaccacg ccccgcaacg
     1921 aattccgtga gcaattcgaa gcgagcgtga agtcggccga caatcagttc ggccacggac
     1981 gcaagaaggg ctcgatgacc gatcccggcc gggtgctgat cgtgttcggc ccgccgaccg
     2041 ccgcgaaacg cacgtcgaag attggtgagg cgaatacaag acagaacatg agagatattg
     2101 agatcggtag cgccatgagt ggctccggct cggcagacat gacctggacg tacgaagccg
     2161 cgctggcgca gaaggccttc gggcttcccc gccttgacgt ggcctttcac gatcaatacg
     2221 gcgaaggaga tttcaagctg accacaccgc tcgtcgatgc gaactcggcg atgacccaga
     2281 aaatgatcgc cagtttcatc acgcaaccgg gcctgacgaa ggctccgacg ttccaggctg
     2341 cgggcgcggc tccggtagtg ccacctcccc cagcggcagc ggccactacg ctcaagaccg
     2401 cagcgctcga agcggcagtc gtcgaagcga agggtggcaa agggaacaat cacggcgccg
     2461 cactcgcgta cgcggagctc atatcgccgg ccggtgagcc gttcgttccc gtctcggttt
     2521 atgtcccggc atccgccggc gttgccgcgg acggtgccga cacgatcttc ggcgcggtcg
     2581 aagatgcgtc gggcaagcgc gtgacgatgt tcgaagagcc ggcgaagctc acggcctcgc
     2641 gcaccgactt cttcgtcgac aagacgttga cgctcgagcc gggcaagtac acggccgtcg
     2701 tcggcctcgc caaagccgga cagccggttc tggtaacgac cggtccgatc gaagtgaccg
     2761 gtccatcgaa ggaagcggtc ggcacgtcgc gcctcatcct ctcggacaac gtctacgagc
     2821 tcggtaccgc cgagcctccg aaatcgccct tcgcatttgg ca
//
LOCUS       DATLVW010000367         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_183639, whole genome shotgun sequence.
ACCESSION   DATLVW010000367 DATLVW010000000
VERSION     DATLVW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183639"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VNN25_11075"
     CDS             complement(<1..198)
                     /locus_tag="VNN25_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004794124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXH92113.1"
                     /translation="MKLVESRETRTDIPAFATGDTVKVHVKVKEGDKERIQIFQGLVI
                     SRKGGGARQMFTVRKISGGIGV"
     gene            complement(343..1077)
                     /gene="trmD"
                     /locus_tag="VNN25_11080"
     CDS             complement(343..1077)
                     /gene="trmD"
                     /locus_tag="VNN25_11080"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXH92114.1"
                     /translation="MRIDFVTIFPSMFEPLLAEGIIARGVKQGLLDVHVWDLRDFATD
                     KHRSTDDEAYGGGPGMVMLAEPVLRCVEAIRADAAEGEPAILMTSPQGRVFTQAAARE
                     LASRSWLIFLCGRYEGFDERIRVALQPDEISIGDFVVSGGELPAMVIADAVGRMIEGV
                     VGNSVSVEEDSFFRGLLDYPHYTRPEELRGMRVPEVLMSGHHEKIRKWRKEQALRATL
                     QKRPDLLDGAELDEEAQAMLRRIREE"
     gene            complement(1067..1600)
                     /gene="rimM"
                     /locus_tag="VNN25_11085"
     CDS             complement(1067..1600)
                     /gene="rimM"
                     /locus_tag="VNN25_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXH92115.1"
                     /translation="MAGDRIALGIIRKAHGVRGEASVEAWSDSPDRFTGLSAVTLVSP
                     DESQTRDAVIESVRIHAGRALVKFAGVNAPEDVRTLQNWTVEVPLTDARTLEADEYFL
                     HDLVGLTLVDAEGRVRGVVAGTEEGGGGVLLNVERDGHRFDVPFAAGIVTKIDLAAKR
                     ALVDLPVGLDDLDAVAD"
     gene            complement(1609..1839)
                     /locus_tag="VNN25_11090"
     CDS             complement(1609..1839)
                     /locus_tag="VNN25_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXH92116.1"
                     /translation="MEELIEFIAKSLVDDADAVNTHVYDRGDQTVVELEVAQADLGKV
                     IGRQGRTARAMRTLLSAAGSKTRRRYTLDIVD"
     gene            complement(1849..2088)
                     /gene="rpsP"
                     /locus_tag="VNN25_11095"
     CDS             complement(1849..2088)
                     /gene="rpsP"
                     /locus_tag="VNN25_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXH92117.1"
                     /translation="MLKIRLRRQGATHSPFYRVVVSDSLKTPSNATVEQIGHYDPKKN
                     PADVKIDRARVDYWVSKGAQLSPTVKSLLKKNKTA"
BASE COUNT          410 a          779 c          698 g          419 t
ORIGIN      
        1 cacgccgatt ccaccagaga tcttacgcac agtgaacatc tgccgggcgc cgccgccttt
       61 gcgcgagatc accagaccct ggaaaatctg gatgcgctcc ttgtcgcctt ctttgacttt
      121 cacatgaacc ttgaccgtat ctcccgtcgc aaatgccggg atgtcggtgc gcgtttcacg
      181 cgattcgacg agcttcatga aatcctgagc catgatgaca accttcctgt gtttctgagc
      241 gagcccgcaa gcatcgccga ttttggcggt ttgggcaacc tcgagactca gattccgtct
      301 ccgggaggct ccgcccgccg tagaaccagc cctccggcgg actcattcct cacgaatgcg
      361 gcgcaacatc gcctgcgctt cctcgtccag ctctgcgccg tccagcagat cgggccgctt
      421 ttgcagcgtc gcgcgtaagg cctgctcctt ccgccattta cggatcttct cgtggtggcc
      481 ggacatcagc acctcgggga cgcgcatccc gcgcagctcc tcgggacgcg tgtagtgcgg
      541 atagtcgagc agaccgcgga agaacgagtc ctcctcaacc gacacgctgt tgccgacgac
      601 cccttcgatc atccggccca ccgcatcggc gatgaccatc gccggcagct cgccgcccga
      661 gacgacgaaa tcgccgatcg agatctcgtc cggctgaagc gccacgcgga tccgctcatc
      721 gaatccctcg tagcgtccgc agagaaaaat cagccacgag cgcgatgcca gctctcgcgc
      781 ggccgcctgc gtgaacacgc gcccctgcgg cgacgtcatg agaatcgccg gttcgccctc
      841 ggctgcgtca gcacgaatcg cttccacgca gcgaaggacc ggctcggcca gcatcaccat
      901 gcccggccca ccgccgtacg cttcgtcgtc cgttgaccga tgcttgtcgg tcgcgaaatc
      961 gcgcagatcc cagacgtgga cgtcgagcag tccctgtttg acgccgcgag cgatgatccc
     1021 ctcggccagc agtggctcga acatcgaggg aaagatcgtg acgaaatcaa tccgcaacgg
     1081 catcgaggtc gtccagcccg acaggcaaat ccacgagagc acgcttcgct gcgagatcga
     1141 tcttcgtgac gatgccggca gcgaacggga catcgaatcg gtggccgtcg cgctcgacgt
     1201 tcaggagcac gccgcctcca ccctcttccg ttccggcaac gacgccgcgc acgcgtccct
     1261 cggcatcgac gagcgtcaat ccgacgagat cgtgcagaaa gtactcatcg gcttcgagcg
     1321 tgcgagcatc ggtcagcggt acttccaccg tccagttctg cagcgtccgc acatcctcgg
     1381 gggcattgac tccggcaaac ttcacgagcg cgcgtcccgc gtgaatgcgg acgctctcga
     1441 tcacggcgtc gcgcgtctgc gactcgtccg gggaaacgag cgttacggcg gagagaccgg
     1501 tgaagcggtc aggagaatcg ctccaggcct cgacgctcgc ttcgccgcgc acgccgtgcg
     1561 ccttgcgaat gatgccgagg gcgatgcggt cgccggccat ggtgcgcctc agtcgacgat
     1621 atcgagcgtg tagcggcggc gcgttttcga gcccgccgcg gacagcagcg tccgcatcgc
     1681 gcgcgccgtc cggccctgac gtccgatcac cttgccgagg tcggcctggg cgacctcgag
     1741 ctcgacgacc gtctgatcgc cgcgatcgta gacgtgcgtg ttcaccgcgt cggcatcgtc
     1801 gacgagcgac ttggcgatga actcgatcag ctcttccatg cagggagctt acgcggtttt
     1861 gttctttttc aggagagact tgacggtcgg cgagagctgg gcgcccttgc tgacccagta
     1921 atcgacgcgc gcgcggtcga tcttgacgtc ggcagggttc ttcttcggat cgtagtgccc
     1981 gatctgctcg accgtggcgt tgctgggggt cttcagcgag tccgaaacca ccacgcggta
     2041 gaacggggaa tgggttgcgc cctggcggcg cagacggatc ttcaacatgt cgtgtgatgc
     2101 tccttcgtga atcgggccgg caatcctacc gaaccggggc gtgaaaccgc aaccgggagg
     2161 ctctcattcc gagggcaggt ccaggcgtcc tcgcgacagc caggcatgct tcagatccgg
     2221 acccgtttca accattggag ttatcaggcc ggatccgaac catcgaggcg ggacgcctcg
     2281 atggccgcgg gcgaagacgc ccgcac
//
LOCUS       DATLVW010000368         4877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_550487, whole genome shotgun sequence.
ACCESSION   DATLVW010000368 DATLVW010000000
VERSION     DATLVW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550487"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="VNN25_11100"
     CDS             <1..1903
                     /locus_tag="VNN25_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXH92118.1"
                     /translation="AAFDNPDLIVACAVGDGEAETGPLATSWHSNKFLNPIRDGAVLP
                     VLHLNGYKIANPTILARIPRSELEHLFLGLGYTPYFVEGSDPDAMHQAMATTLEHCVM
                     EIRALQAAARSTGRVERPAWPMLVLRSPKGWTGPKTVNGHKVEDFWRAHQVPILDPAT
                     NPKSLQILEEWMRSYKPEELFDAAGTLKPELRQVAPQGARRISANPHANGGRLRKPLN
                     LPRFQNYAVPVPSPGKSYVSPTATLAGFLAEVMRRNMQTFRVFGPDETASNKLEGIYA
                     ASKKTWVAEYKPEDSDGTEIAVDGRVMEMLSEHTLEGWFEGYVLTGRHGFFSSYESFV
                     HVIDSMFNQHAKWLEKAKKDLDWRAAISSINLLITSLVWRQDHNGFTHQDPGFLDVVT
                     NKSPDVVRIYMPPDANCLLSVADHCLRSKDYFNVIVADKQPHLTLLDMDAAVNHCTKG
                     IGIWDWASTDAGEEPDVVLACAGDIPTMEALAAVSLLKEHIPALKLRFVNVIDLFRLV
                     PDTEHPHGMSDRDFDSLFTPDKPVIFNFHSYPSLIHKLAYRRTNHVNFHVRGYKEKGN
                     INTPLELAILNQVDRFNLAIDVIDRVPALQRTAGHVKDRLRDAIIEHLAYAHAEGIDK
                     PEIRDWQWP"
     gene            1942..3141
                     /locus_tag="VNN25_11105"
     CDS             1942..3141
                     /locus_tag="VNN25_11105"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HXH92119.1"
                     /translation="MSKGIVVINVGSTSIKFAAYRHGGAEALELLCRGRLEDIGSRPR
                     FVAHDSQGKPMAGDAPNAGYPLDPRGITQFVITWLEAHLQDFKIDALGHRVVHGGSFY
                     DKPVRVDEQVITRLERLAIIEPSHQPFEVSTIRAVAQAHPELQQVAVFDTAYHRTLPA
                     VAQRYALPAAIAGEEIRHWGFHGISYEYLARELPRHAPKARRVIAAHLGGGAGMCAML
                     DGKSMDTSMGFSVIEGLPMSTRCGAIDPAILLYLMKNKKYTADDLESLLYKDSGMLGL
                     SGSSGDMRLLREHYSPSAAAAIDYFVHHIVKFAGAYAAVLGGLDALVFTAGIGENDPT
                     LRAAVVQRLAWLGARLDDGANSRNGPRISTEDSEVSVWVIPTNEELMIARHTAAAIDE
                     RRERPDA"
     gene            complement(3144..4316)
                     /locus_tag="VNN25_11110"
     CDS             complement(3144..4316)
                     /locus_tag="VNN25_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH92120.1"
                     /translation="MIWVMRHSQSHGREVARRFAGALGVLVAVVSALADPVPQAGAPI
                     KPTSRGEATAIIADARKILTPEGVETLEKVRIGGIEQWVSVRGVDRRNPVLLYIHGGP
                     GYVSLPMSWWSTRGWEEYFTVVQWDQRAAGKTHLLTNPDIVAPTLTNERMVADAEEMA
                     LWARKAFGKRKIFVLGHSYGSYLGLELARRHPDWLHAYIGVGQVANISESERRGWQFA
                     MDAARREGNTEAVRQLESIAPYPAGDKPSPIQDTYIERKWMTYFGGAMAYRRTNDAED
                     DLAYLSPDYTDDEIRHLWDGNAFSTPYLLPDLLAERNTATKLDVPLVVFVGRHDKNVN
                     ADVSAEWFARVRAPEKHLVWFEHSAHLLITEEPGKALISLVRYARPIAERAGDVAP"
     gene            complement(4345..>4877)
                     /locus_tag="VNN25_11115"
     CDS             complement(4345..>4877)
                     /locus_tag="VNN25_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH92121.1"
                     /translation="LRGQVHDGRFAFGLYQYLGFVEGFKRPQFHVSEWIAPGSKIDLG
                     DRNLTIESLPGHTPDSVVLLDEAANRLFAGDFIYPSEIYAFLPGADLKTYAASARRLT
                     KLVDAQTLIYGAHGCDRLPTVDVPTLSQADVVALTRALDLADTGAWAAGTGWYPRRIP
                     VNERMELLAKYPWMRP"
BASE COUNT          996 a         1504 c         1446 g          931 t
ORIGIN      
        1 ggccgccttc gacaatccgg acttgatcgt cgcctgcgca gtcggggatg gcgaggcaga
       61 aacgggaccg ctggccacct cctggcattc caacaaattc ctaaatccca tccgcgatgg
      121 ggccgtactg ccggtgctgc atttgaacgg ctacaagatc gccaatccga cgattctcgc
      181 gcgaattccg cgcagtgaac tcgagcacct gttccttgga ctcggctaca caccttactt
      241 cgtggagggc agcgatcctg acgccatgca tcaggccatg gccacaacgc tcgagcactg
      301 tgtaatggag attcgcgctc tccaggccgc agcccgcagc accggccgcg tcgaacggcc
      361 ggcgtggccg atgctcgtac tgcgctcgcc gaaaggctgg accggaccca aaacggtgaa
      421 cggccacaaa gtggaggact tttggcgcgc gcatcaagtt cccattctcg atccggccac
      481 gaatccgaag agcctgcaaa tcctcgagga gtggatgcgc agctacaaac ccgaggaatt
      541 gttcgacgcg gccggaaccc tgaagcccga acttcggcag gtggcacccc agggggcgcg
      601 gcgcatcagc gccaatcctc atgccaacgg cggcaggttg cgaaagccat tgaacctgcc
      661 gcgttttcaa aactatgccg tgccggtccc gagtcccgga aaaagctacg tttccccgac
      721 cgcgacactc gccggcttcc tggccgaagt catgagacgc aacatgcaga cgtttcgcgt
      781 ctttggtccg gacgagaccg cttcgaacaa actcgagggg atctacgcgg catcgaagaa
      841 gacgtgggtg gcggaatata aaccggagga ttccgacgga acggagattg cggtcgacgg
      901 acgggtcatg gaaatgctca gcgagcatac tctcgaaggc tggttcgagg gctatgtgtt
      961 gaccggacga cacggcttct tctccagcta cgagtccttt gttcacgtca tcgactcgat
     1021 gttcaatcaa catgccaagt ggctggagaa ggccaagaag gatctggact ggcgggccgc
     1081 gatttcctcg atcaatctgc tgattacttc gctggtgtgg cgccaggatc acaatggctt
     1141 tacgcaccag gacccgggct tcttggatgt cgtcaccaac aagagccccg atgtcgttcg
     1201 aatctatatg ccgccggatg cgaattgcct gttgagcgtc gccgatcatt gtctgcgttc
     1261 aaaagattat ttcaacgtca tcgtcgccga caaacagccg cacctcacac ttctcgatat
     1321 ggatgcagct gtcaatcact gcaccaaggg catcggaatc tgggattggg ccagcaccga
     1381 tgcgggcgag gaacccgatg tcgtcctggc gtgcgccggt gatattccca cgatggaagc
     1441 attggcggca gtgagcctct tgaaggaaca cattccggcg ctgaagctgc gctttgtgaa
     1501 tgtgatcgat ctgtttcgcc tggtaccgga caccgagcac ccgcatggca tgtcggatcg
     1561 agacttcgat tcgctgttca ccccggacaa accggttatc ttcaatttcc acagttatcc
     1621 atccctcatc cacaagctcg cctatcgccg aaccaaccat gtgaatttcc acgtccgcgg
     1681 ctacaaggag aaaggcaaca tcaatacacc ccttgaattg gcgatcctca accaggtgga
     1741 tcgcttcaat cttgcgatcg atgtcatcga ccgtgtcccc gcgctgcaaa ggaccgcggg
     1801 gcacgtgaag gaccggttga gagatgccat catcgagcat ctcgcctacg cccacgcgga
     1861 aggaatcgac aagccggaaa ttcgggactg gcaatggcct tagggcggag gcgcttgccc
     1921 caggaaggca actgaggcgt catgtcgaaa ggtattgtcg tgatcaatgt tggatccacc
     1981 agcatcaagt ttgccgcgta ccgccatggg ggcgccgagg ccttggaatt gctctgccgc
     2041 ggccgcctcg aagacatcgg ctcgcggccg cgcttcgttg cccacgactc gcaaggcaag
     2101 ccgatggccg gggacgctcc caacgcggga tatccgctcg acccccgggg catcacccag
     2161 ttcgtgatca cctggctcga agctcatctg caggacttca agatcgacgc actgggtcat
     2221 cgggtggtgc acggaggttc gttttacgac aagccggtgc gggtcgatga acaggtcatc
     2281 acccggctcg agaggctcgc gatcatcgaa ccttcgcatc agccgtttga ggtcagcacg
     2341 atccgcgcgg tcgctcaggc tcatcccgaa ttgcagcagg tggcggtgtt cgacaccgcg
     2401 tatcaccgga ccctgcctgc ggtcgctcag cgttacgcac ttccggccgc aatcgcggga
     2461 gaggaaattc gccactgggg tttccacgga atctcgtatg agtacctcgc tcgcgaattg
     2521 ccgcgccacg caccgaaggc ccgccgggtc attgccgctc acctgggcgg cggcgccggc
     2581 atgtgcgcca tgcttgacgg caaaagcatg gacacatcga tggggtttag tgtaatcgag
     2641 ggcctcccga tgtcgactcg ctgtggtgca atcgatcccg cgatcttgct ctacctcatg
     2701 aagaacaaaa aatacaccgc ggacgacctt gagagcctgc tctacaagga ctcagggatg
     2761 ctgggactct ctggaagcag cggcgacatg cgtctactga gggaacacta cagtccgtcg
     2821 gccgccgcag ccatcgacta tttcgtccac cacatcgtga aattcgccgg cgcctatgcg
     2881 gccgtgctcg gtggactcga cgcgttggta ttcacggcgg gaatcggcga gaacgacccg
     2941 acgttgcgtg ctgcggtggt gcaacggctt gcgtggctcg gcgcaaggct cgatgacggg
     3001 gctaacagcc gaaacggccc gcgaatctcc accgaggaca gcgaggtctc cgtttgggtc
     3061 atcccgacga atgaggaact gatgatcgcg cggcataccg ctgccgccat cgatgagcgt
     3121 cgcgaacggc cggacgcgta gccttaggga gccacgtccc ccgcccgctc ggcgatcggt
     3181 cgggcgtagc gcacgaggga aatgagggct ttgcccggct cttcggtgat cagcagatgt
     3241 gccgaatgct cgaaccagac gaggtgcttc tccggcgccc gcaccctcgc aaaccactcg
     3301 gccgagacat cggcgttcac gttcttgtcg tggcgcccta cgaacacaac gagtggaaca
     3361 tcgagtttgg ttgccgtgtt ccgctcggcg agtagatcgg gaagcaagta aggggttgag
     3421 aacgcgttcc cgtcccagag atggcgaatc tcgtcatccg tatagtcagg cgacaagtag
     3481 gcgagatcgt cttccgcgtc gttggtcctg cgatacgcca tcgctccgcc gaagtaggtc
     3541 atccacttgc gctcgatgta ggtgtcctga atcgggctgg gcttgtctcc cgccggataa
     3601 ggagcgatcg attccagctg gcggactgcc tccgtattcc cctcccgccg cgcggcgtcc
     3661 atggcgaact gccagccgcg acgttcgctc tcagatatgt tcgcgacctg gccaacaccg
     3721 atgtacgcgt gcagccagtc gggatggcgc cgcgccaact cgaggccgag atagcttccg
     3781 taggaatgtc cgaggacgaa gatcttgcgc ttgccaaagg ccttgcgcgc ccatagggcc
     3841 atctcttcgg catcggcgac catgcgctca ttggtgagag tcggggcgac gatgtcggga
     3901 ttggtcaata gatgggtctt gccggccgca cgctggtccc actgaaccac cgtgaaatat
     3961 tcctcccatc cgcgcgtcga ccaccaactc atcggcaggc tcacatatcc cggaccgccg
     4021 tggatgtaga gcaataccgg atttctgcga tcgacgccgc ggaccgacac ccattgctca
     4081 attccgccga tccgcacctt ctccagcgtt tcgacgcctt cgggagtcag gatcttgcgt
     4141 gcgtcggcaa taatggctgt ggcttcacca cggctcgtcg gtttgatcgg cgccccggcc
     4201 tgggggaccg ggtcggccaa agcagagaca acggcgacca gaactcccaa ggcgccggca
     4261 aacctgcgag cgacctccct accatgactt tgcgagtggc gcataaccca gatcataacc
     4321 gcgtgagctc gacaggtgca taggctatgg ccgcatccat ggatacttcg ccagtagctc
     4381 catccgctcg ttcacaggta tgcgccgcgg ataccagccg gttcccgcgg cccacgctcc
     4441 ggtgtcggcc aagtcgagag cgcgtgtcag cgccaccaca tcggcctggc tcaatgtggg
     4501 tacgtcaacc gtcggcagcc gatcgcagcc gtgagcgccg taaatgagag tttgtgcatc
     4561 gaccaacttc gtcagccgcc gggcgcttgc cgcataggtt ttcaggtctg caccgggaag
     4621 aaacgcgtag atttcggacg gatagatgaa gtctccggca aaaagccggt ttgctgcctc
     4681 gtcgagcagc acgactgaat ccggagtatg cccgggcagg ctttcaatgg tcaggttacg
     4741 gtcgccgagg tcgatcttcg atcccggcgc aatccactcc gacacatgaa attgcggacg
     4801 cttaaagcct tcgacgaagc cgagatactg gtaaagcccg aacgcaaagc ggccatcgtg
     4861 tacttggccg cgcaacg
//
LOCUS       DATLVW010000369         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_349573, whole genome shotgun sequence.
ACCESSION   DATLVW010000369 DATLVW010000000
VERSION     DATLVW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349573"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VNN25_11120"
     CDS             <1..1003
                     /locus_tag="VNN25_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH92122.1"
                     /translation="TGGNTMTRIRTAAIAAVLGLASISAAHAAIDSPIHRNFNVHAGG
                     TITIDADLGDIKVTSGASNVSVDVIRRAKTSSSERANELFKDFDVQFSQAQNDVHIHA
                     RYNRPTSWSWFHWNNDLAVRFVVNVPSQYNVDLTTSGGDIAISDLAGRVKVRTSGGDV
                     TLGRIDGIVDAHTSGGDVSMAGSHAAATLSTSGGDIKVGDASGSLSARTSGGSIDIRR
                     ATADLKAHTSGGSIDIGDAGGAIDASTSGGSIKARVSRQPHADSRLSTSGGGITIHVA
                     PNVALDIDAHTSGGDVASDVPVTIQGKQDDSSLNGKLNGGGPKLVLRSSGGDIRLAR"
     gene            1854..1982
                     /locus_tag="VNN25_11125"
                     /pseudo
     CDS             1854..1982
                     /locus_tag="VNN25_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            complement(2057..3346)
                     /locus_tag="VNN25_11130"
     CDS             complement(2057..3346)
                     /locus_tag="VNN25_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXH92123.1"
                     /translation="MSKNVIIVGGGLAGLSAAVFLARAGRTVTIFERRRYLGGRAVTH
                     LRHGYRFNLGPHAFFRAGHGWNVLRELGIPVRGGVPKVRGFGMLGTQRFKFPGSLWSI
                     LTTSLFGLKAKMQMLALLLRIRGIDPKPYASMTVREWIDANASDERVRLLLEALFRLA
                     TYSNRPDLQSAGSALAQLRLSIRGVVYIDEGWQKIVDSLHSAAVASGVNFVTSSRVVS
                     VDNEGGAVRGVELGGLEVEDRKDTMSLVMPDMSDPDKGTRIPADTVLLAVDPSTAREL
                     VRDVDWPETTAVVMSSLDIALSKLPQPHNTFALGLDKPHYFSVHSAWAQLTPKGGALL
                     HVGHYGDGGTEAELEALVDELQPGWRDLVVHRRFLPAMTVSNALVQPDGVAARTKAVT
                     PVRGLYVAGDWVDEGGILSDAAFASARAAAKAIIAAG"
BASE COUNT          662 a         1125 c         1106 g          626 t
ORIGIN      
        1 cacaggaggc aacaccatga cgcgcattcg cactgcagcg atcgcggctg tgctcgggct
       61 ggcgtcgatc tccgccgcgc acgccgcgat cgacagcccc atccaccgca acttcaacgt
      121 tcacgccggc ggaacgatca ccatcgacgc cgatctcggc gatatcaagg tgacctcagg
      181 cgcgagcaac gtcagcgtcg acgtgatccg ccgcgcgaag acgtcgagca gcgaacgggc
      241 gaacgagctg ttcaaagact tcgacgtcca gttctcgcag gcgcaaaacg acgttcacat
      301 ccacgcccgc tacaaccgcc cgacctcgtg gtcgtggttt cactggaaca acgacctcgc
      361 ggtccgcttc gtggtcaatg tcccctcgca gtacaacgtc gatctcacaa cctccggcgg
      421 cgacatcgcc atctccgatc tcgccggacg cgtaaaggtc cgcacttccg gcggcgacgt
      481 gaccctcggc agaattgacg gcatcgtcga cgcgcacacc tcgggaggcg atgtttcgat
      541 ggccggctcc cacgcggccg cgacgctctc cacctccgga ggcgacatca aagttggcga
      601 cgcctccggt tcgctgagcg ccaggacctc gggaggatcg atcgacattc gccgcgcaac
      661 cgccgatctg aaggcacaca cgtcgggcgg ctccatcgat atcggcgacg ccggcggtgc
      721 catcgatgcc tcgacctcgg gaggatcgat caaagcgcgt gtttcacgtc agccgcacgc
      781 cgactcgcgg ctctcgacct cgggtggcgg gatcacgatc cacgtcgcgc ccaacgttgc
      841 gctggacatc gatgcacaca ccagcggcgg cgacgtcgcc tccgatgtgc cggtcacgat
      901 tcagggcaag caggacgact catcgctgaa cgggaagttg aacggcggcg gaccgaaact
      961 cgttctccgc agctctggcg gcgacatccg cctcgcgcga tagccccgcg gtagccccag
     1021 cgttagccct tttgcgttag ctcgcttttc ccacactctt atcgcaactc tctccatcga
     1081 gcgccttctc ccccacgctt cttgtggggg agaaggtggc gaagccggat gagggggcgt
     1141 tccaacgagt ccggcatctg cttatgtgcc acgttttcag cgagttcagg cgaggagtaa
     1201 ttgatcgccc tgcgcgcttg ctgccccctc atccgccttc ggcaccttct ccccccgcaa
     1261 aaagcgcggg gggagaaggc gctcgattgg agagcactcc aaagaaaaag gaaagacgta
     1321 tgcgcaccgt gagaaatgcg cgttcgcgcg acgtgggcgt ggccgaaagt ccgcacggca
     1381 gagagtggta cgttgtccgc aacgtgaata caccggttgg accccggaag agtgccttta
     1441 aggctgacgc cgtagtcgcg tatctcatac tgggcgcggt agtttgctac ctggttgcag
     1501 gcgtgattca ggggcctcgt cagggcgcgc tctgcggaga tggttgggag agcacggctg
     1561 tcggacgagg cgcgtgttcc catcatcacg gcgtttcgca atggcgtcac gtgaacgacg
     1621 aatcggctga accggtttgg atatccgggc attcgtgtct cgttggtgca atcctaactt
     1681 tcatctgcgt gcaagtaggg caccgcaacc gaaaggcgcg gctcgcagca gtctcaccgt
     1741 tgcccgctcc caaattgcaa cgctcttctt ccagaaatcg actctctcca cctcgagaga
     1801 gatcgtccgc tccccgagca gaatcctccc cgtcaaggtc agatggttca gcatgtccca
     1861 gatgcggctg tccgctggtt cagcgggtgc gaaagcgcga cgggcacgca ttcctaggtt
     1921 gttcagagtt cccgcgttgc cgctacacgt gttccgtcgt ggcagacggc ggcttgtcgt
     1981 aaggccgagt gttgggtaag cacaagatca agagcggtac gaccagacgc acaaacgctg
     2041 agaagacttc gatccgtcag ccggccgcga taattgcttt tgcggcggcg cgcgcgctgg
     2101 caaaggcggc gtccgagagg atgccgcctt cgtcgaccca atcgccggca acgtagaggc
     2161 cgcgtacggg agtgaccgcc ttcgtccgcg ctgctacgcc gtccggctgc acgagcgcat
     2221 tggaaacggt catcgcagga aggaaacggc gatgcacgac gaggtcgcgc cagcccggtt
     2281 gcagctcatc gaccagcgct tccagctccg cctccgttcc gccgtcaccg tagtggccga
     2341 cgtgcagcag tgcgccaccc ttcggggtca actgagccca cgccgagtga acggagaagt
     2401 agtgcggctt gtcgagcccg agtgcgaagg tgttgtgcgg ctgcggcagc ttcgagaggg
     2461 cgatatcgag cgacgacatc accacggccg tcgtttccgg ccagtcgacg tcgcgaacga
     2521 gctcacgggc ggtggaggga tcgaccgcca ggaggaccgt atcggcgggg atgcgcgttc
     2581 ccttgtcggg atccgacatg tccggcatga cgagcgacat cgtgtcctta cggtcttcca
     2641 cttcgagtcc gccgagctcc acgcctcgca cggcgccgcc ttcgttgtcg acgctgacga
     2701 cgcgcgagct ggtgacgaaa ttcacgcccg acgccacggc ggcgctgtga agcgaatcga
     2761 cgatcttctg ccagccttcg tcaatgtaga ccacgcctcg aatcgagagc cgcaactgcg
     2821 caagcgcgga gcccgcgctc tgaagatcgg ggcgattgct gtaggttgcc agccggaaga
     2881 gagcctcgag caggagccgc acgcgctcgt cggacgcgtt cgcatcgatc cattcacgaa
     2941 cggtcatcga tgcgtacggc ttggggtcga tgccgcgaat gcgcaggagg agcgccagca
     3001 tctgcatctt cgctttgagt ccgaacagcg acgtcgtcag gatcgaccag aggcttccgg
     3061 gaaacttgaa ccgctgcgtg cccagcatcc cgaagccgcg gaccttgggc acgccgcctc
     3121 gcaccgggat gccgagctcg cgcagcacgt tccagccgtg ccccgcgcga aagaacgcgt
     3181 gcggaccaag gttgaaacga tagccgtggc ggagatgcgt gacggcgcgg ccaccgaggt
     3241 atcggcgccg ctcgaagatc gtaacggtgc ggccggctct tgcgagaaag accgcggccg
     3301 agagtccggc cagaccgcca ccaacgatga tcacattctt cgacacggtg aaatcggatg
     3361 ctatcgcgat ttggtgccgg gttggtgggt tggcgggtta ttcggctgcc ggttgggcgg
     3421 ttacccagca acccatgaag agtatgcttg acgcctgctc catggtgctt tgatcgattc
     3481 tcatgctccg gaaggagggt gcgttctgcg aaaacttgt
//
LOCUS       DATLVW010000370         5786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_425971, whole genome shotgun sequence.
ACCESSION   DATLVW010000370 DATLVW010000000
VERSION     DATLVW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425971"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..294)
                     /locus_tag="VNN25_11135"
     CDS             complement(25..294)
                     /locus_tag="VNN25_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HXH92124.1"
                     /translation="MMQEVRMTNVEVTFPDSLFAALRKAPHEVGREVRLAASIHWYQQ
                     GAISMERAAEFAGLSLPQFLAELARRHVDVFLVDDEDIARELRDA"
     gene            538..3039
                     /locus_tag="VNN25_11140"
     CDS             538..3039
                     /locus_tag="VNN25_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH92125.1"
                     /translation="MAPSTLGISRSTLALTVALLSTAASAATGPAPERVSGYDLPPQY
                     VLDVLHAPAPPRPILSPTRGSALLVSWVQYPAMAQVAEPFLKLAGVRVEPRSRRKHDT
                     FGGYGVAQCAQTLSVVDLATRHETRIALPPGGCADGFSWAADGATFAFRNTSSDAVEL
                     WAGARDGTTHRVGNVRLNPMLGNSHQWAPDGKTLLVKLVPDNIGAPPAVEAGAEGPRI
                     QESTGGGEFSTYEARDTLRNQADAALFEYYGTSQLALVDIASGSVARVGAPAVLSDVH
                     LSPDGEHILVTSIRKPYSYAVTYSRFANDVEVWDRFGKATPLATLPVADHVPIHGVAT
                     GPRDHEWRPTEPATLVWAEALDGGDWKTSVPARDKVLMQSAPFTAKPVEIARTEQRYQ
                     GIDWSEQRGTGLLQEYDDNRHWRRTFLIDADHTETKPRLIWDLSSDETYDDPGQPVYR
                     ILPNGQWAIRQKGDTIFLSGQGASTTGDRPFLDSFDLRTVKPQRLFRSGKDAYEYFLT
                     FANDNTDALLTWHQTPSEPPNLMLRTLGAPVTSAAKGEATIASTVRAITQIPDPTPAV
                     RAIKKRLVTYKRKDGVALSFTLYTPPGYKEGTRVPAILNAYPLDYASSKTAGEVSGSE
                     QTFTRLTDYKLLLLSGYAIIDNAAFPIVGDPKKAYDTYIDQLVDDAQAAVDQAVELGV
                     VDRDRIGVTGHSHGALMTANLVAHTDLFRAGVATSGAYNKTLTPFGFQNESRSVWNAQ
                     DVYLKVSPYFFANKMKAPLLLVHGSDDANPGTTPLQSEQLFQAIRGNGGVVRLVMLPH
                     EPHWYAAMESNEQLAYEELRWFDKYVKNAEPRKHD"
     gene            complement(3311..4798)
                     /locus_tag="VNN25_11145"
     CDS             complement(3311..4798)
                     /locus_tag="VNN25_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH92126.1"
                     /translation="MNRKTIALAILALTILAGTNLAAADDFADVKREIAKRHDEAVKR
                     LQDWIALPSIAAEDRNYPAGAEYMAKLALDAGFQHATVLSTDGKPGVFATLDAGAPKT
                     VGLYFMYDVKQFDPAEWTSPPLEARLVDKPNIGKSVVGRGAVNQKGPEAAFLAALHAI
                     RGAGRKMPVNLVLVAEGEEEIGSPHIGQLVHRPEVEAAFRKTTGVFMPAASQDLDGIV
                     TVSLGAKGVVELELVSSGEKWGRGPAKDIHSSLKAMVDSPAWHLIKALDTLVSADGNT
                     ITIDNYPQPRLLNADEKAMIANASKLRSEAQSKKSLGVQHWIGDLPWVEANERLESQP
                     TVNIEGLVGGYTGPGGKTILAHRAVAKIDMRLVPDMKKDDAVAALKAHLAKRGFGDIE
                     VNVTGGYDPTSTSAAAPLIQSQVAVLKRAGIDPVLWPRNAGSYPGYVFTNPPLSLPSG
                     HFGLGHGSGAHAPDEYYVIDSTNPKVQGFDGAVMSFVEYLYELGR"
     gene            5091..>5786
                     /locus_tag="VNN25_11150"
     CDS             5091..>5786
                     /locus_tag="VNN25_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXH92127.1"
                     /translation="MIFRAPEAPVAIPDVALTPFLLERAASWGDKAAMIDSTNGRVLT
                     YAGLADAVRRAATSLARRGLRKGDVFAIYSANSPDYAIAFHAVSLLGGIVTTINPMYT
                     VEELTRQLQDAGASYLVAGPSCLDKALESAKSLTLREVFVFGEAAGATPFDSLLDSDD
                     DVPSVTIDPQNDVVVMPYSSGTTGLPKGVMLTHRNLVANILQGEAVFGLPAGAVTLGV
                     LPFFHIYGMVVIMN"
BASE COUNT         1050 a         1899 c         1804 g         1033 t
ORIGIN      
        1 ggcgacgcat tgatcaccag cgtttcaggc atcgcgcaat tctcgggcta tgtcctcgtc
       61 atcgaccagg aaaacatcca catgacggcg agcaagctcc gccaggaact gagggaggct
      121 cagtcccgcg aattcggcgg cccgctccat cgagatagca ccctgctgat accaatggat
      181 cgacgctgca agacggacct cgcgcccgac ctcatggggc gcctttcgga gcgcagcgaa
      241 caaactatcg ggaaacgtga cctcaacgtt ggtcatccgc acctcctgca tcatcgatct
      301 tagagtttac gccagcgaaa cgagctacgc cgcgcatcgg tcgacaggcc tggtactcgc
      361 ggcgagcccc acatgatcat gtattcgcaa gcagagctac attcacccac gcgagccact
      421 actggggcca cgccttcgct cgttgattcg accggtcaag atcgaaggtc tcgggtcaca
      481 ctcactcttg tgctcaacgt atgaactcgg tgcaccgttc tgtcagataa tctcttcatg
      541 gcgccttcaa ccctagggat ctctcgcagc actctcgctc tgacggtagc tctcctctcg
      601 actgcggcat cggccgcgac cgggccggcc ccggagcgcg tctccggcta cgacctgccg
      661 ccgcaatacg tgctcgacgt cctgcacgcg cctgcgccgc cgcggccgat cctgagtccg
      721 acacgcggga gcgcacttct cgtgtcatgg gttcaatacc cggcgatggc gcaggtggcg
      781 gagccatttc tgaaactcgc cggagtgcgc gtcgagccac gctcccgccg caagcacgac
      841 acgttcggcg gctatggagt cgcacagtgc gcgcaaacgc tctccgtcgt ggacctcgca
      901 acacgtcacg agacgcggat cgcgctgccg cccggcggct gcgccgacgg cttctcgtgg
      961 gccgccgacg gcgccacctt cgcgttccgc aacacgtcca gcgacgctgt ggagctctgg
     1021 gccggcgccc gtgacggcac cacgcaccgc gtcggcaacg tgcggctcaa cccgatgctc
     1081 ggcaactcac accagtgggc gcccgacggc aagacccttc tggtcaagct ggttcccgac
     1141 aacattggcg cgccgccggc ggtagaggcg ggagccgagg gcccgcggat tcaggagtcg
     1201 accggaggcg gcgagttcag cacctatgag gcgcgcgaca cgctgcggaa ccaggccgac
     1261 gcggcgctct tcgagtacta cggcacgagc cagctggcac tggtcgacat cgcgagcggc
     1321 tcggtcgccc gtgtcggtgc gcccgcagtc ttgagcgacg ttcacctctc gcccgacggc
     1381 gagcacattc tggtgacctc gatccgcaaa ccgtattcgt atgcagtgac ctatagccgc
     1441 tttgcgaatg acgtcgaagt atgggaccgc ttcgggaagg cgacgccgct cgcaacgttg
     1501 cctgtcgcgg atcacgtgcc gatccacggc gtagcgaccg gtccacgcga ccacgaatgg
     1561 cgtcccaccg agcccgcgac actcgtttgg gcagaggcgc tcgacggcgg agactggaag
     1621 acgagcgtac ccgcgcgcga taaagtcttg atgcaaagcg cgccgttcac agccaagccc
     1681 gtcgagatcg cccgcaccga gcagcgatac cagggtatcg actggagcga gcaacgtgga
     1741 accggattgc tccaggagta cgacgacaac cgtcactggc gccggacgtt cctgatcgac
     1801 gccgatcaca cggaaacaaa gccgcggttg atctgggatc tgtcgagcga cgagacgtat
     1861 gacgatccag gccagccggt ctaccgcatc ctgccgaacg gccagtgggc catccgtcag
     1921 aaaggagaca cgatcttcct gagcggccag ggtgcttcaa cgacggggga ccgtccgttc
     1981 ctcgacagct tcgatcttcg taccgtcaag ccgcaacgtc tcttccggag cggcaaggac
     2041 gcgtacgagt acttcctgac cttcgccaac gacaacaccg acgcgcttct cacatggcac
     2101 cagaccccct cggaaccgcc caacctcatg ctgcgcacac tcggggcgcc cgtgacgagc
     2161 gctgccaaag gcgaagcgac cattgcctcg accgtgcgcg ccatcacgca gatccccgac
     2221 ccgacgcccg ccgtgcgtgc aatcaagaaa cgcctggtga cctacaagcg aaaggacggc
     2281 gtcgcgctct cattcaccct ttacacgccg cccggctaca aagagggcac gcgggtgccg
     2341 gcgatcctca atgcctaccc actcgactat gcgagctcca aaaccgcggg tgaggtcagc
     2401 ggctcggagc agaccttcac acgcctgacg gattacaaac ttctgctcct atccgggtac
     2461 gcgatcatcg acaacgcggc attcccgatc gtcggagatc cgaagaaggc ttacgacacg
     2521 tacatcgatc agctcgtgga tgacgcgcag gccgcggtgg accaggcggt ggagctcggc
     2581 gtcgtcgatc gggaccgcat cggtgtcacg gggcacagcc acggggcgtt gatgaccgcg
     2641 aatctggtcg cccacacgga tctgttccgg gcgggcgtcg cgaccagtgg tgcctacaac
     2701 aagacgctga cgccgttcgg tttccagaac gagagccgtt cggtatggaa cgctcaagat
     2761 gtctatctca aggtctcgcc ctacttcttc gccaacaaga tgaaggctcc cctgctcctc
     2821 gtccacggct ccgacgatgc aaaccccggc acgacgccgc tgcagtccga gcagctcttc
     2881 caggcgattc gtggaaacgg gggcgtcgtc cgtcttgtca tgctgcctca cgagccgcac
     2941 tggtacgcgg ctatggagtc gaacgaacaa cttgcgtacg aggagctgcg ctggttcgac
     3001 aagtatgtga agaacgcgga gccgcggaag cacgactgag tgaaccgtgg tggtcgagtt
     3061 agagtggcca ccgagacgcg gcaccacccg cccgcgcatt cccgagccga agcactacaa
     3121 cctcggcgca cacgttcggt aaagccgaag gtgtgatcca cgaagttgac ccaaaaaaac
     3181 atggcgggta tcaggccgga ttaaaccatc gaggcgggga cgccccgatg gccgcggtcg
     3241 agccctccgg agcctcggaa accgagcgat ggcttgcccg cacggtctag tgattcgagt
     3301 tctcaagaca ctacctcccc aattcgtaca gatactccac gaacgacatc accgcgccgt
     3361 cgaagccttg cactttaggg tttgtcgagt cgatcacgta gtactcgtcc ggtgcatggg
     3421 cgccgctgcc gtggccgagg ccgaagtgtc cgctggggag gctgagcggt ggattggtga
     3481 agacgtagcc tggatacgag ccggcgttgc gcggccagag gacgggatcg atgccggcgc
     3541 gcttcaggac agccacttgc gactggatca gcggtgcggc ggcggaagtg ctcgtcggat
     3601 cgtagccacc ggtgacattc acttcgatgt cgccgaagcc gcgtttggcg aggtgcgctt
     3661 tgagcgcggc gacagcatca tccttcttca tgtcggggac gaggcgcatg tcgatcttgg
     3721 ccacggcgcg atgcgcgagg atcgtcttgc cgcccggacc ggtgtagccg ccgacgaggc
     3781 cttcgatgtt cacggtcggc tgcgattcca gccgttcgtt cgcctcgacc catgggagat
     3841 cgccgatcca gtgctgcacg ccgagggact tcttcgattg cgcttcgctg cggagcttcg
     3901 acgcgttggc gatcatcgcc ttctcatcgg cgttgagtag ccgcggctgc ggatagttgt
     3961 cgatcgtgat cgtgttgcca tctgccgata cgagcgtgtc gagcgccttg atcagatgcc
     4021 aggccgggct gtcgaccatt gctttgagcg atgaatggat gtcctttgcc ggaccgcgtc
     4081 cccacttctc gccgctcgac accagctcca gctcgacgac acctttcgcg ccgaggctga
     4141 cggtgacgat gccgtcgagg tcttgcgagg cggcgggcat gaatacgccc gttgtcttgc
     4201 ggaacgcggc ctccacctcg gggcgatgga cgagctggcc gatgtgcggc gagccgatct
     4261 cttcctcacc ttcggcgacc aggacgaggt tcaccggcat cttccgtccc gcccctcgga
     4321 tcgcgtgcag cgccgcgaga aacgccgctt ccggaccttt ctgattgacc gcaccgcggc
     4381 cgacgaccga cttgccgatg ttcggtttgt cgacgagccg cgcttcgagc ggcggggagg
     4441 tccactccgc gggatcgaac tgcttgacgt cgtacatgaa gtagaggccg accgtcttcg
     4501 gcgcgccggc gtcgagcgtg gcaaagactc cgggcttgcc atcggtgctg aggacggtgg
     4561 cgtgctggaa cccagcatcg agcgccagct tggccatgta ctccgcgccg gcgggatagt
     4621 tgcgatcctc ggcggcgatg gacggaagcg cgatccaatc ctgcagccgt ttgaccgctt
     4681 cgtcgtgacg tttggcgatc tcgcgtttga cgtcggcgaa gtcgtcggcg gccgcgaggt
     4741 tggtgccagc aaggatggtg agtgccaaaa ttgcgagagc gatcgtcttg cgattcatcc
     4801 cgcgatgtta cttcatcgcc ggaggagtgc ggtcgtctcg accgcgggca tttgggcgtc
     4861 tcgcccgaat gctcttggta ggcaaacgcg cggacggagg ctttgcgcga gggcttcatt
     4921 cggagcttga ctcgacccac cgttgaatac tcttcggttg gctgtctcgc ggtcggacgt
     4981 ttcgcccaaa agtgtccatt cggagctgcc gggcgggcca caccgccggg cgaggacgcc
     5041 cgacggccgc gggcgaagac gcccgcactc ctcgggtacc attaatgtta atgatcttcc
     5101 gcgctcccga agctccggtt gccatcccgg acgtcgcgct cacgccgttt ctgctggagc
     5161 gcgctgcgtc gtggggcgac aaagcggcga tgatcgacag cacgaacggg cgcgtgctca
     5221 cgtacgccgg attggccgac gcggtgcgcc gcgcggctac gtcgctggcg cgccgcggtc
     5281 tgcgcaaggg cgacgtcttc gcgatctaca gcgcgaactc tcccgactac gccatcgcat
     5341 tccacgccgt ctcgctcctc ggcggcatcg tcacgacgat caatccgatg tacacggtcg
     5401 aggagctgac gcgccagttg caggatgccg gcgcgagcta tctcgtcgcc ggaccgtcat
     5461 gtctcgacaa ggcgctcgaa tctgcgaaga gtctcacgct tcgcgaagtg ttcgtcttcg
     5521 gcgaggcagc gggcgcgacg ccgttcgatt cgctgctcga cagcgatgac gacgtaccgt
     5581 ccgtcacgat cgatccgcag aacgacgtcg tggtcatgcc ttactccagc ggcacgaccg
     5641 gactgccgaa gggcgtgatg ctcacccatc gcaacctcgt cgcgaacatc ctgcagggcg
     5701 aagcggtctt cggtcttcct gcgggtgccg tcacgcttgg cgttctcccc ttctttcaca
     5761 tctacggcat ggtcgtgatc atgaac
//
LOCUS       DATLVW010000371         8374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_550588, whole genome shotgun sequence.
ACCESSION   DATLVW010000371 DATLVW010000000
VERSION     DATLVW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550588"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..802
                     /locus_tag="VNN25_11155"
     CDS             212..802
                     /locus_tag="VNN25_11155"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXH92128.1"
                     /translation="MITLPSVADVHLPSSWSLGEFRLRPLRAGDAPAWLAYLSDPRVI
                     EHTSFPELDLSGVEAMLSREIEGYAAGTSCRWALADATDGLIGTCGFSNWSLPHAHAE
                     LVYDLAPAFWGRGLMRAAAHQALSWAFNTARFNRVHAFVMTSNAPSIRLLESLGFARE
                     GMLQQYRVARGVPRDFYIYARLRAEASEAGGAPPDS"
     gene            894..1445
                     /locus_tag="VNN25_11160"
     CDS             894..1445
                     /locus_tag="VNN25_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92129.1"
                     /translation="MYEAVELAEEDLPALQAFFDANPEYFQAVTGAPPRPDEARQEFE
                     SRPPPSVPYDKVHILGFLDSSGQMVAMASVMTGLLAPRVWHISLFIVATALHGTGTAG
                     LLYERLEEWAKDGGASWLRLGVVVGNLQAERFWEKAGYKEVQRVPEQLGSMTHTARVM
                     IKPLGSLDVDEYLREVPRDRPKS"
     gene            1544..2218
                     /locus_tag="VNN25_11165"
     CDS             1544..2218
                     /locus_tag="VNN25_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HXH92130.1"
                     /translation="MSIMMPRVFISSTSEFATERDQLKQEIEALPDLRLDAYIYEAEA
                     AGTEAPEQRLRKVLQGSEIFLLILGDKFGSEYPGRATSIVEWEYDYAKTEKKELKGYV
                     KDPLGPNADPRQAAFIARAVAFRDGSWIRRFHDTPQMISAAIGDLRKWITDAGLAWRS
                     GQSERNQWKDRFVLISGAVVALVTIAGVVIATLESVPMEKLVLIFACGVSMFAGLWLF
                     LKSDVF"
     gene            2231..3034
                     /locus_tag="VNN25_11170"
     CDS             2231..3034
                     /locus_tag="VNN25_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92131.1"
                     /translation="MTTAPYDESVQAAVARETKRRKRVFSTFMALLLVPIAIGAYALS
                     KAPTEAQKVATDVAPIVTQRVGADISAGITNNVMAQTQPLIQKNVAREISANVEPRLV
                     AVDTLKQDITNLQQTTQKVSDFVASATPQLQSVSGLGDRLSTLQGLVDQHDNTFKTIS
                     SDQASLRQDLSKTRESTQALSMRIDKLATVPNDLEASRRQMQEFQQAVTKELGSIRNM
                     VNSDVQAANANKEAIGSINGRLSRIENDLSKLQARANVIERSKDEKKPQ"
     gene            3095..3589
                     /locus_tag="VNN25_11175"
     CDS             3095..3589
                     /locus_tag="VNN25_11175"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HXH92132.1"
                     /translation="MPIPVVIDRLAEELDALMDETSLYLNRQTGEFLTVPDHDLGVIE
                     DELEDEEELEWPWEGEDLQKLRDIAETENWVCLPSKLDVNEWRIMQRFALEMPEPLSS
                     TLEHAIHGRGAFRAFRSVLEGTGRIEAWYQFKHEHLVRLVAEVLEDENIPFKRGRAYE
                     PGEE"
     gene            3570..3845
                     /locus_tag="VNN25_11180"
     CDS             3570..3845
                     /locus_tag="VNN25_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92133.1"
                     /translation="MSPAKSERPLYRAIFVFVLAPVAAAVVVSVLLLFGVSPHAVFYI
                     GFVLRSWLEYLGKPVPNAVGVIAALSFWWVVIVAIGLLWDWRRQRNN"
     gene            complement(3865..4500)
                     /locus_tag="VNN25_11185"
     CDS             complement(3865..4500)
                     /locus_tag="VNN25_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXH92134.1"
                     /translation="MSIHSAMARQASTITAPPETARGTTKRERILRAAIEVFAQNGYF
                     NAKVSEIAKSAGVADGTIYLYFDGKEDLLVTVFREHTRNYLQSLEKALAHVRRPEERI
                     RIAIRHHLETLGRDRQLAIVAQVELRHSLKFMSLLSQQEVADYLNMLRKMIEHGQSDG
                     SFRRTIHPQLGAKAVFGILDEMVTSWILSEKDYDLAASADAIADLVLTGLL"
     gene            complement(4519..4926)
                     /locus_tag="VNN25_11190"
     CDS             complement(4519..4926)
                     /locus_tag="VNN25_11190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXH92135.1"
                     /translation="MTCVFCHDPCDAGDLVSEDEKLLVILHSDWAVRGHAMIVWKEHV
                     ENVADLAPDDYAHFAAVHHRVEKALLDVTHADRAILLKLGIVTPHLHLHIYPVRATLD
                     RAQVMEIIDGKTVDAMSEGQRKAFIEEIRQRLA"
     gene            5051..6019
                     /locus_tag="VNN25_11195"
     CDS             5051..6019
                     /locus_tag="VNN25_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH92136.1"
                     /translation="MRTIRFGRSNVQVPAISLGTWGHGGPSTSDGASVGWSGHDDRLA
                     KAALVEAHRQGITHWDTADAYGRGHAEELIGEAFAEGVPRKEIFLASKFGWRKREGSH
                     WYEPSYMREQCEASLRNLQTDVIDLYYFHHCDFGPNDEYFVDALATMRQFRDEGKIRF
                     IGLSDWDGRKIMKFIDRVDPDVVQPYRNVIDDDYESSGLKAWVDKHDAGVAFFSPLKH
                     GLLLGKYDEPQHFPEGDFRSGIPEFGDPEFLVRMRYARDEMTRRFADQREPVLHALTG
                     ALLAGNPTATILLGQRNPKQVDAAAAVGSALNVADAAWVRRVYRGI"
     gene            6163..6645
                     /locus_tag="VNN25_11200"
     CDS             6163..6645
                     /locus_tag="VNN25_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92137.1"
                     /translation="MTLPQFAPGFAEMLRELSANGVDFLVVGAHARAAYGDPRATKDL
                     DIWVRPTAENAQRVWAALETYGAPLTDLEVSDFASPGITFRIGIAPYRIDILTELTGL
                     TFEDAWPNRVTASFAGGAYPVIGKHDFLTNKRATGRPQDLADVDHVERAELDADGEPQ
                     "
     gene            complement(6639..>8374)
                     /locus_tag="VNN25_11205"
     CDS             complement(6639..>8374)
                     /locus_tag="VNN25_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXH92138.1"
                     /translation="DTRVDPRPPTHDSRVAISFVGEGTTSLGEWHEAINACAARKLPA
                     IFCVQNNQTAISTSPSEQSAVRVFADKALGYGIPAITIDGTDPDAIAAAFTWAVERAR
                     AGNGPALIELVAMRMCGHAHHDDMLYLGKDPQQSWSYPPLTENGYADHERYEYWRSKD
                     PIPSYAAKLQAEGIIEAGDLERFQREAEALVEAEARAVIEAPWPDPELAGTGVLANEP
                     RRVHIEVLDESWRQNASKNIGLPAVETAPQFDAKGKTFLEAVMLGVGDALRSDPRTFV
                     YGEDVGGKYGNAFLLLKPLVKDFGDRIVNSILSEGATLGVCTGAALAGMRPIGEIQFN
                     DFVASGFNQLVNNAAKIRYRWGGSVPMVVRMPWGGLRHAGPYHSQNTEAWFYRTPGLK
                     IVAPSTPHDARALMASAVADPDPVLYYEHIALYRDPRVKQMLADEAPSPLPIGKAALR
                     RAGNDLAIISYGAYVHIGMRVAETLAKDGIESSVLDLRSLAPLDRDAVLAVARHCSRV
                     LIILEDTRTGSIGESLAAIIQEEAFEYLDAPIRILGALDTPVPYSPPLEDEFLASEKE
                     IERAARLLVGY"
BASE COUNT         1556 a         2490 c         2678 g         1650 t
ORIGIN      
        1 gaggtcacag agatccctct gtgttctctg tgtctccgtg gttgcctctg cctcggcgac
       61 cacgataagc agtccaaatt gaagccacga cttggagccg gcgccgtgcc ggtcgctgcc
      121 atgttccgcg ttaccatgaa cgcttgtcgc tctagccacg cgagtactgt gcggctgatc
      181 ggcaaacgtc aggcgtcgcg gacaaacgat cgtgattacc ttaccctctg ttgcagacgt
      241 gcatttgccc tcgtcatggt cgctgggcga gttccggctt cgtccgctcc gcgccggtga
      301 tgcgccggcg tggcttgcgt acctctctga tccgcgtgtc atcgagcaca ccagctttcc
      361 cgagctcgac ctctccgggg tcgaagccat gctttctcgc gagatcgagg gctacgcggc
      421 tggtacctcg tgtcgctggg ctcttgctga cgcgacggat ggcctcatcg gtacctgcgg
      481 gttctccaat tggtccttgc cgcacgctca cgccgaactg gtctacgacc tcgcaccggc
      541 attctggggc cgcggtctaa tgcgcgccgc cgcccaccag gcgttgtcct gggcgttcaa
      601 cactgcacgg ttcaaccgcg tgcatgcgtt tgtcatgacc agcaacgccc catcgatacg
      661 gctcctggag tccctcggct ttgcgcgcga agggatgctg cagcagtacc gcgtagcgag
      721 aggcgtgccc cgggactttt acatctatgc ccggcttcgc gcggaagcga gcgaggctgg
      781 cggcgctccg cctgattcat aggcgcaagt tgctgtatcc cgcgtgcgtt actatttcgt
      841 tagagcgcgt gaactcatcc aacctgttac cgcgtcgacg attcgcggtc ggcgtgtacg
      901 aagctgtgga gttggccgag gaagatctgc ctgccctgca ggccttcttt gacgccaatc
      961 cggagtactt ccaggcggtc actggagctc cgcctcgacc cgatgaggcc aggcaggagt
     1021 tcgagtcacg gccacctccg agtgtgccat acgacaaggt gcacatcctc ggcttcttag
     1081 actccagcgg tcagatggtg gcgatggcct ccgtgatgac cggcctcctg gcaccacgcg
     1141 tgtggcacat cagccttttc atcgtcgcca cggcattgca tggcacgggc acggccgggc
     1201 tgttgtacga acgactcgag gagtgggcga aggatggagg cgcgtcctgg ctgcgcctcg
     1261 gtgtcgtggt cggcaacctg caggctgaac ggttctggga gaaggccggc tacaaggagg
     1321 ttcagcgcgt acccgaacag cttggcagca tgacccacac tgcgcgggtg atgatcaagc
     1381 cgcttggaag cctggatgtc gatgagtacc tgcgcgaagt tccccgcgat cggccgaagt
     1441 catagtcttg ccgagaatta aggttcagcc gactcgcgcc gctccctgcc gcggcgggta
     1501 gggaaaccac ccgcctgaac actcctaaaa tggagagtca acgatgtcga tcatgatgcc
     1561 gcgggtgttc atcagctcga ccagcgaatt cgcgactgag cgcgatcagc tgaagcaaga
     1621 gatcgaggcc ctgccagacc tccggctcga tgcctatatc tacgaggccg aagcggcggg
     1681 aaccgaggcg ccggaacagc ggctcaggaa ggtgctccaa ggctcggaga tctttctgct
     1741 catcctcggc gacaagttcg gctccgagta tcccggacgt gcgacgtcga tcgtggagtg
     1801 ggagtacgac tacgcgaaaa cggagaaaaa agagctgaag ggctatgtca aagatccgct
     1861 gggcccgaat gctgatccgc gccaggcggc cttcatcgcg cgcgcggtcg cctttcgcga
     1921 cggcagctgg atccgcaggt tccatgacac accacagatg atcagcgccg caatcggcga
     1981 tttgagaaag tggatcaccg atgcgggatt ggcctggagg agcggccagt ccgagcgcaa
     2041 tcagtggaaa gaccgctttg tcctcatcag cggcgctgtg gtagcgctgg tgacgatcgc
     2101 cggcgtggtc atcgctacgc tcgagagtgt cccgatggag aaactcgtcc tcatattcgc
     2161 gtgtggcgta agcatgtttg ccggtttgtg gctattcctg aagtctgatg tcttctgaag
     2221 gaggatctcg atgacgactg caccttatga cgagtccgtt caagcggcgg tagcgcgtga
     2281 gacgaaacgc cgcaaacgtg tgttttcaac cttcatggca ttactgctcg tgccgatcgc
     2341 catcggcgcg tacgcgctca gtaaagcgcc cacagaagcg cagaaggttg ccaccgatgt
     2401 cgcgccaatc gtcacgcaac gcgtaggcgc cgatatcagc gcaggcatta cgaacaatgt
     2461 tatggcccaa acgcagccgc tcatccagaa gaacgtcgcg cgcgagattt ccgcaaacgt
     2521 cgagcctcgg ctcgtagcag tcgacacgct caaacaagac attacgaacc tgcagcagac
     2581 aacgcagaag gtatcggact tcgttgccag cgcgacgccg caattgcaat ccgtatcggg
     2641 gctcggcgat cgtctttcca cgcttcaggg actggtcgat cagcatgaca atacattcaa
     2701 gaccatcagt agcgaccagg cgagccttcg tcaggacctt tcgaagaccc gcgagtcgac
     2761 tcaggccttg tcaatgagga ttgacaagct tgcgacggtg cccaatgacc tggaggcatc
     2821 ccgcaggcag atgcaggagt ttcaacaggc ggtgacgaag gagctggggt cgatccgcaa
     2881 catggtgaat tccgatgttc aggcagcgaa tgccaacaag gaggccatcg gatcgatcaa
     2941 cggccggctt tcccgcatcg aaaacgattt gagtaaattg caggctcgtg ccaacgtaat
     3001 cgagcgctcc aaggacgaga aaaagccgca gtagttcttc attcggacgt gtgcgttaga
     3061 attggctcct ggcttgagcc cgttagccgc tgacatgccg atacccgtcg taatcgaccg
     3121 actcgccgaa gagcttgatg ccctgatgga tgagaccagc ctgtacctga atcggcagac
     3181 tggcgaattc ctcaccgttc ccgaccacga tctcggcgtc atcgaggatg agctcgaaga
     3241 tgaagaggag ctcgagtggc cgtgggaagg cgaggatctc cagaagcttc gcgacatcgc
     3301 tgaaactgaa aactgggttt gtttgccatc aaagctcgac gtgaacgagt ggcgaattat
     3361 gcagcgcttc gcactcgaaa tgccggagcc gctgtcgtca actctcgaac acgcaatcca
     3421 cggcagaggt gcctttcgcg cgttccggtc cgtgctcgag ggtaccggac ggatcgaggc
     3481 ctggtatcaa ttcaagcacg agcatctggt tcgcctcgtg gccgaggttc tggaagatga
     3541 gaacattccg ttcaagaggg gacgcgctta tgagcccggc gaagagtgaa cgtcccctct
     3601 accgcgccat tttcgtgttc gtgcttgccc cggtcgcggc ggcggtcgtg gtcagcgtgc
     3661 tcctgctgtt cggagtcagc ccccacgccg tcttctacat tggctttgtc ctcaggtcgt
     3721 ggcttgagta tctcggcaag ccggttccga acgcagtcgg cgtgatcgcg gcgttatctt
     3781 tctggtgggt agtgatcgtt gcgattggtc ttttgtggga ctggagacgg cagagaaaca
     3841 attgacgttg ctcgggacga gtccctacaa caatcccgtc aacaccagat ccgcgatcgc
     3901 atcggcgctg gcggcgaggt cgtaatcctt ctcggaaaga atccacgatg tcaccatctc
     3961 gtcgaggatg ccgaagacgg ctttggcgcc aagctgcggg tggatcgtgc gccggaagct
     4021 gccgtcggac tggccgtgct cgatcatctt gcggagcatg ttgagatagt cggccacttc
     4081 ctgctgcgag aggaggctca tgaacttgag gctgtggcgc agctcgacct gggcgacgat
     4141 ggccagttgc cggtcgcgcc cgagggtctc gaggtggtgg cggatggcga tgcggatccg
     4201 ttcctcgggg cgtctcacgt gcgcgagcgc cttctccagc gactgcaggt agttgcgcgt
     4261 gtgctcgcgg aagaccgtga cgagcagatc ctctttgcca tcgaagtaga ggtagatcgt
     4321 gccgtcggcg acgccggccg atttggcgat ctcggagact ttggcgttga agtagccgtt
     4381 ctgggcgaag acttcgatgg cggcgcggag gatgcgctcg cgtttggttg tgccgcgggc
     4441 ggtctcggga ggagcggtga tggtggatgc ttgccgggcc attgctgaat gaatgctcat
     4501 tcagtcggcc tgcatttgtc aagcgagccg ctgccggatc tcttcaatga acgctttccg
     4561 ctgcccttcg ctcatcgcat cgactgtctt gccgtcgatg atttccatca cctgcgcgcg
     4621 gtcgagagtg gcgcggaccg gatagatgtg caggtgaagg tgcggcgtga cgatgccgag
     4681 cttgagaaga atggcgcggt cggcgtgcgt cacgtcaagc agcgctttct cgacgcggtg
     4741 atggacggcg gcaaagtggg cgtagtcatc gggcgcaaga tcggccacgt tctcgacatg
     4801 ctccttccag acgatcatgg cgtgaccgcg cactgcccag tcgctgtgca ggattacgag
     4861 gagcttctcg tcctcggaga caaggtcgcc ggcgtcgcaa gggtcgtggc agaaaacgca
     4921 ggtcatcttc gcgatgactc tatctcacgc gaagacgcga agacgcgaag acgcgaagac
     4981 gcgaagacgc gaaggaaacg agccgaagcc ttcttcgcgt cttcgcggct tcgcgtgaaa
     5041 caatctcaac atgcgaacaa tccgatttgg ccgctctaac gttcaggttc cggcgattag
     5101 cctcggcacg tggggacatg gtggtcccag tacttcagac ggtgcgtccg tcggttggag
     5161 tggtcacgac gatcgcctcg caaaggccgc gctcgtcgaa gcgcaccggc agggcatcac
     5221 gcattgggat accgccgacg catacggccg cgggcacgcc gaggagctga tcggggaggc
     5281 gttcgcggag ggagtgccgc gcaaagagat cttcctcgct tcgaagtttg gttggcggaa
     5341 acgtgagggg agtcattggt acgagccgtc gtacatgcgg gagcagtgcg aggcttcgct
     5401 gcgcaatctg cagacggacg tcatcgacct ctactacttt caccactgcg atttcgggcc
     5461 gaacgatgag tacttcgttg acgcgctggc cacgatgcgc cagttccgcg atgagggaaa
     5521 gatccgcttc atcgggctgt cggattggga tggccggaag atcatgaagt tcatcgatcg
     5581 cgtcgacccc gacgtggtgc agccgtatcg caacgtcatc gacgacgact acgaatcgag
     5641 cggcctcaag gcctgggtcg acaagcacga tgccggcgtc gccttcttct ctcccctgaa
     5701 acacggcctg ctcctcggga agtacgacga gccgcagcac tttcccgaag gcgactttcg
     5761 cagcggcatc ccggagtttg gcgatcccga attcctggtc cgcatgcgct acgcccgcga
     5821 cgagatgacc cgccgtttcg ccgatcagcg cgagccggtg ctgcacgcgc tgaccggcgc
     5881 gcttctcgcc ggcaacccga ccgccaccat cctcctcggc cagcgcaatc cgaagcaggt
     5941 cgatgccgcg gcagcagtcg gaagcgcgct gaatgttgca gatgcggcgt gggtgcgaag
     6001 ggtctaccgc gggatataat tcgggcgtgg cgcgacccgg aattaccgtg cggaaataca
     6061 agatgggcga ggagccgctt attgatgaag agacgcgccg gatgacggca ggcgagcgga
     6121 tcgagcttgc ctggcatgtg actctttcgg cttgggagat gtatgaccct gccgcagttc
     6181 gcgccgggtt tcgccgaaat gttgcgcgag ttatccgcca acggcgttga cttcctggtc
     6241 gtgggtgctc atgcgcgcgc tgcctatggc gacccgcggg ccacgaagga tctcgacatc
     6301 tgggtccggc ccaccgcgga gaacgcgcag cgggtgtggg ctgcgctgga gacttacggt
     6361 gccccgctta ctgacctcga agtgtcggat ttcgcgtccc ccggaatcac attccgaatc
     6421 ggaatcgcac cgtatcggat cgacatcctc actgagctga ctggtttgac tttcgaggat
     6481 gcgtggccga accgcgtcac tgcctcattt gccggaggcg cgtacccggt gatcggcaag
     6541 catgactttc tcaccaacaa acgtgcgacg ggccggccac aggatctggc tgacgtggat
     6601 cacgttgagc gtgcggagct tgacgccgac ggtgagcctc agtaacccac cagcagccgc
     6661 gccgcccgct cgatctcctt ctcgctcgcc agaaactcat cttcaagcgg cggcgaatac
     6721 ggcacgggcg tgtcgagggc gccgaggata cggatggggg cgtcgaggta ttcgaaggcc
     6781 tcttcctgaa tgatcgccgc gagcgactcg ccgatcgagc ctgtgcgcgt gtcttcgagg
     6841 atgatcagga cgcggctgca atgtctcgcg acggcgagca ccgcgtcgcg atcgagcggt
     6901 gcgagcgagc ggagatcgag tacagacgat tcgatcccat ccttcgccag tgtctccgcg
     6961 acgcgcattc cgatgtgcac gtacgcgccg tacgagatga tcgccagatc gttgccggca
     7021 cgtcgcagcg cggctttacc gatcggaagc ggcgacggcg cttcatccgc gagcatctgc
     7081 ttgaccctcg ggtcgcgata cagcgcgatg tgctcgtagt acagaaccgg atcgggatcg
     7141 gcgacggcgc tggccatcag cgcgcgtgca tcatgcggcg tcgacggtgc gacgatcttc
     7201 agcccgggcg tccgatagaa ccacgcttcc gtgttctgcg agtgatatgg accggcgtga
     7261 cgcaaaccgc cccacggcat gcgcacgacc atcgggaccg acccgcccca tcggtagcgg
     7321 atcttcgccg cgttgttgac tagttgattg aagccgctcg caacgaagtc gttgaactgg
     7381 atctcgccga tcggccgcat tcccgccagc gccgccccag tgcaaacgcc gagcgtcgcc
     7441 ccctcagaga gaatcgaatt cacgatccgg tcgccaaagt ctttcaccag cggtttgagg
     7501 aggaggaagg cgttgccgta tttgccgccg acgtcttcgc cgtagacgaa cgtgcgcgga
     7561 tcgctccgca gcgcatcgcc gacgccgagc atgacggctt cgaggaacgt ttttcctttc
     7621 gcatcaaact gcggcgcggt ctcgaccgca ggcaagccga tgttcttcga cgcgttctga
     7681 cgccagctct cgtcgagcac ttcgatgtgg acgcgccgcg gttcgttggc gagtacgccg
     7741 gtgccggcca gctccgggtc cggccatggc gcttcgatga ccgcgcgcgc ctcggcttcg
     7801 acgagtgcct ccgcctcgcg ctgaaagcgc tcgaggtctc ccgcttcgat gatgccctca
     7861 gcctgcagtt tcgccgcgta ggacgggatc ggatctttgc tgcgccagta ctcataccgc
     7921 tcgtggtcgg cgtagccatt ctccgtgagc ggcggatagc tccacgattg ctgtgggtct
     7981 ttgccgaggt agagcatgtc gtcgtgatgg gcgtggccgc acatgcgcat ggccacgagc
     8041 tcgatcaacg ctgggccgtt gccggcgcgg gcgcgctcga ctgcccaggt gaaagccgcg
     8101 gcaatcgcat cgggatcggt gccgtcaatg gtgatggcgg ggatgccgta tccgagggct
     8161 ttgtcggcaa agacgcgaac ggcggattgc tcgctcggcg acgtcgagat cgcggtctgg
     8221 ttgttctgca cgcagaaaat cgccggcagc ttccgcgccg cgcaggcatt gatcgcctcg
     8281 tgccattcgc caagggatgt ggtgccttcg ccgacgaaag agatggctac tcgggagtcg
     8341 tgagtcgggg gtcgggggtc tacgcgggtg tcgg
//
LOCUS       DATLVW010000372         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_59636, whole genome shotgun sequence.
ACCESSION   DATLVW010000372 DATLVW010000000
VERSION     DATLVW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59636"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VNN25_11210"
     CDS             <1..973
                     /locus_tag="VNN25_11210"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXH92139.1"
                     /translation="LAHKLIEAVQAAAGSFAYRDLREYKPVWRAPVRIDFRGYEIYAP
                     APPAAGGLALGTVLKIVSGLDLGTGGLQSAKTLHLLLEAERRAAIDRDKFLGDPAGGR
                     VPMHDLLSDERAAFWRASIDPKKVTPTIAITEPGTVTTTSGHTTHVSIVDAHGNVVSL
                     TTSLGDDGGSGFVVPGLGVLLNNAMSNFSSAPNTPNSLQWGNRPASPLSPVIVLRGGK
                     PVLAIGTPGGPTITTTIAQILLDMFVFKRPLAEAIAAPRWSQQATPEEISYEQGRAPQ
                     ATLDALLALGHGITARDAIGDVQAVLIEGRKLRAISDSRHRGAAGGY"
     gene            complement(1097..1378)
                     /locus_tag="VNN25_11215"
     CDS             complement(1097..1378)
                     /locus_tag="VNN25_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92140.1"
                     /translation="MPEQIIPISEQIIPMPEQIIPIPEQIIPMPERIIPMSEQIVPMP
                     QCAIPMSEQIVSMPECAVPISQPPGSIARQAELRGNCLKTRHMHWWSTH"
     gene            1686..2336
                     /locus_tag="VNN25_11220"
     CDS             1686..2336
                     /locus_tag="VNN25_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92141.1"
                     /translation="MSAPTPPPVTSSPAVTAAATADAIAEQFRAVLVTLLAIVPDLQK
                     YDSKKIKRIAASAKFGNDAIVPVINLVASVPRMKESNLFDVDAGHFARQFKDQVVPLA
                     KRLIEFANDVVFTSENLLSVSSVQALQMYRLGQHVVKQPDGADLQPFVDEMSHIVKKT
                     MNIRKKPAPAPTPTPTTSHIPPGAQGLLASNLVQDKTGEPEDVADRFEQALKDVTK"
     gene            complement(2402..3082)
                     /gene="rpiA"
                     /locus_tag="VNN25_11225"
     CDS             complement(2402..3082)
                     /gene="rpiA"
                     /locus_tag="VNN25_11225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HXH92142.1"
                     /translation="MTAEEQKIEAAREALRSIHSGMSLGLGTGSTAKEFVALLGDALA
                     RGELSNIRCTCTSNQTEEQARSLGIPLFPLAEIAPLDIAVDGADEIDAQLRLIKGRGG
                     ALLREKIVEQQAKRFIVIADESKIVERLGTGVLPVEVTPFALEVLEERFSVMGLAPVL
                     RLRDGQPRITDEGHRILDVRVPAEHDIADIVASIRELAGVVETGFFPTEAAEALIATS
                     NGVRRMTR"
     gene            complement(3185..>3946)
                     /locus_tag="VNN25_11230"
     CDS             complement(3185..>3946)
                     /locus_tag="VNN25_11230"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92143.1"
                     /translation="CQTASITSQPTANPATVVFGSNATLSIGVTGTSNVIQWFNSSNV
                     FIGNGASIQVTPTATTTYYALVSNPCTSTLTSANVTVTVCFLPNITTQPAATPSTIDA
                     GGSSTLSIGVSGATTVQWYTGTTLAGTGTSVIVTPPGTSGKTIYYAVVSNSCGNVQSN
                     NVGVTICTPGLGTTFTATPSTITSGQSSKLEVGGATGTATLTYLFYKSDGTFVGSSTN
                     KKLFVSPAVTTSYYYKVSNSCGVTDPSPTITVTVQ"
BASE COUNT          736 a         1205 c         1206 g          799 t
ORIGIN      
        1 cctcgcccac aaactgatcg aagccgttca ggctgcggcc ggttcgtttg cgtaccgcga
       61 tctgcgcgaa tacaagccgg tctggcgcgc gccggtgcgg atcgattttc ggggttacga
      121 gatctatgcg cctgcgcctc cggccgctgg aggtctcgcg ctcggtacgg tgttgaagat
      181 cgtcagcgga ctcgatctcg gcacgggggg gttgcaatca gcgaagacgt tgcaccttct
      241 gcttgaagcc gagcggcgtg ccgcgatcga ccgcgacaaa tttctcggcg atcccgccgg
      301 cggacgcgtg ccgatgcacg atcttctctc tgacgagcgc gccgcgttct ggcgggcttc
      361 gatcgatccg aagaaagtga caccgacgat cgcgatcacg gagccgggca cggtcacgac
      421 gacatctggc catacgacgc acgtctccat cgtcgatgcg cacggcaacg tcgtgtccct
      481 gaccacctca ctcggcgacg acggaggaag cggcttcgtc gtgccgggcc tcggcgtttt
      541 gctgaacaat gcgatgagca atttctcctc cgcaccgaac acgccgaact cgttgcagtg
      601 gggcaaccgt cctgcgtcgc ccctttcgcc ggtgatcgtg ctccgcggcg gaaagccggt
      661 cctcgccatc ggcacgccgg gaggcccaac gatcacgacg acgatcgcgc agatccttct
      721 cgacatgttc gtattcaagc gcccgctcgc ggaagccatc gccgcgccgc ggtggtcgca
      781 gcaggcaacg ccggaggaga tttcctacga gcagggacgc gcgccgcagg caacgctcga
      841 tgccctcctg gcgctcggcc acggaatcac tgcccgcgac gcgatcggcg acgttcaagc
      901 cgtgttgatc gagggaagaa aactccgggc gatctccgat tcgcgtcaca gaggggccgc
      961 gggcggatat tagccggatc tccagccgcg cgccgttcaa caaccttctt cggcgtctcg
     1021 gagtctccca tcgagcgcct tctcccccac gctttttgtg ggggagaagg tggccgaagg
     1081 ccggatgagg gggcgtctaa tgagtgctcc accagtgcat gtggcgtgtt ttcaggcagt
     1141 tcccgcgaag ttctgcctgt cgcgcgatcg aacccggcgg ttgcgatatc ggaaccgcgc
     1201 attccggcat cgaaacgatt tgttccgaca tcggaatcgc gcattgcggc atcggaacga
     1261 tttgttccga catcggaatg atccgttccg gcatcggaat gatttgttcc ggtatcggaa
     1321 tgatctgttc cggcatcgga atgatttgtt ccgatatcgg aatgatctgt tccggcatcg
     1381 gaatgatttg ttccgaaatc ggaatgatct attccggcat cggaacaaaa cattccgact
     1441 tattaccgcc ctttcccgcc ttccagccac cgatgccggc ttattcgccg cttcgatctc
     1501 ctggcgcgtt tttccctcag ctgaggaacc ttcgtccggg cggcgcgtct caccttatgt
     1561 ctgaaccgtt ttcaggccac gccgtcagcg tttcgcgggc gacgtgattg gcaggcccgt
     1621 ggcggccaca cgatgaacga cgacacgcgt tgcaaatcga gcgcgtgcct taaccggaga
     1681 tcaagatgtc agcaccaacc cctcccccgg tcactagtag tccagcagta actgcggccg
     1741 cgacggccga tgcgattgcc gagcagtttc gcgccgtcct tgtcacgctc ctcgcgatcg
     1801 tgcctgatct tcagaagtac gactccaaga agatcaagcg cattgcggcg agcgccaagt
     1861 tcggaaacga cgccatcgtc ccggtcatca acctggttgc cagcgttcct cgtatgaagg
     1921 agagcaacct gttcgacgtc gatgccgggc actttgcgcg tcagttcaag gatcaggtcg
     1981 ttccgctggc aaagcggtta atcgagttcg ccaacgatgt cgtcttcacc agcgagaacc
     2041 tgctttccgt ctcgtccgta caggcgcttc agatgtaccg cttggggcaa cacgtcgtca
     2101 agcagccgga cggcgctgac ttgcaacctt ttgttgacga gatgtcgcac atcgtcaaga
     2161 agacgatgaa catccgcaaa aagccggcac cggcaccaac tcccacccca acgacgtctc
     2221 acatcccgcc tggcgctcag ggcctcctgg cgtcgaatct agtccaagac aaaaccggcg
     2281 agccggaaga tgtcgcggac cgtttcgaac aggcattgaa agacgtcacc aagtagccat
     2341 gaagttacgg ggggcatgct gccacgagca tgcctctcgt tcctttcggc cagtcgggtc
     2401 cttaccgcgt catccggcgt acgccgttac tggtggcgat gagcgcctcg gccgcttccg
     2461 tcggaaagaa tccggtctct accactcccg ccagctcacg aatactggcc acgatgtcgg
     2521 cgatgtcgtg ttccgcaggc acccgaacgt cgaggatgcg atgcccttcg tcggtgatgc
     2581 gcggctggcc atcgcgaagg cgaagcacgg gggcgaggcc cattaccgag aatcgctcct
     2641 ccagaacctc gagcgcgaac ggcgtcactt cgacaggcag cacgccggtg ccgaggcgtt
     2701 cgacgatctt cgactcatcg gcgatgacga tgaatcgctt cgcctgctgc tcgacgatct
     2761 tctcgcgcag aagcgcgccg ccacggcctt tgatcagccg aagctgcgca tcgatctcgt
     2821 cggcgccatc gacagcgata tcgagaggcg cgatctcagc gagcgggaag agcgggatcc
     2881 caagcgagcg cgcctgctcc tcggtctgat tcgacgtgca ggtgcaacgg atgttgctca
     2941 actcaccccg ggcgagcgcg tcgccgagga gcgcgacgaa ctctttggcg gtgcttcctg
     3001 tcccgaggcc gagggacatg ccgctgtgaa tggatcggag cgcctcgcgg gctgcctcga
     3061 tcttttgctc ttctgctgtc atgccttcaa gtgtattcgc aggtggacgt tctacgcatt
     3121 caaacaaaga actgccgcca ctctcgcaag tggcggcagc ttcgaaccaa agactattga
     3181 acgactattg aaccgtgacc gtgatggtcg ggcttggatc tgtgacgccg caggaattgc
     3241 tgaccttgta gtaatagctc gtggtcacgg ccggcgagac aaacagcttc ttgtttgtcg
     3301 aggagccgac gaacgtgcca tcgctcttgt agaagaggta agtcagagtg gccgttcccg
     3361 ttgcgccgcc cacttccagc ttcgagctct ggcctgaggt gatcgtcgaa ggcgtggcgg
     3421 taaacgtcgt gccgagtcct ggcgtgcaaa tcgttacccc gacattgttg ctttgaacat
     3481 tgccgcaaga gttgctcacg acggcgtaat agattgtctt tccagacgtt cccggaggcg
     3541 tgacgatgac gctggttccg gtgccggcga gcgtggtgcc ggtgtaccac tgtacggtcg
     3601 ttgctccaga gacgccgatg gagagcgtgg aactgccgcc ggcgtcgatc gtcgatggag
     3661 tggccgccgg ttgcgtcgta atgttcggca ggaagcagac cgtgacggtg acgttcgcgc
     3721 tggtcaaggt cgatgtgcat gggttgctga ccagggcgta ataggttgtc gtggccgttg
     3781 gggtgacctg gatgctggca ccgttgccga tgaacacgtt cgaggagttg aaccactgaa
     3841 tgacgttcga cgtgccggtc acgccgatcg atagcgtggc attgctgccg aagacgaccg
     3901 tcgccggatt ggccgtgggc tgcgaggtaa tcgaggcagt ctggca
//
LOCUS       DATLVW010000373         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_142848, whole genome shotgun sequence.
ACCESSION   DATLVW010000373 DATLVW010000000
VERSION     DATLVW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142848"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VNN25_11235"
     CDS             complement(<1..297)
                     /locus_tag="VNN25_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92144.1"
                     /translation="MTDGTRILRTAGIPLIIAVALLYVVPKECSKVLEISKQRQAARA
                     SSGLHIESSQKPVTYPAGLDAERIRYVVEIDSQFSAPYMGRVRKSAALVLLPREQ"
     gene            complement(294..674)
                     /locus_tag="VNN25_11240"
     CDS             complement(294..674)
                     /locus_tag="VNN25_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH92145.1"
                     /translation="MSSSLSNACVLILDDNPLVLKLGRALLEREGCTVLTAVSWIEFN
                     HVLASNSPDIIFLDVNLPSIKGNRLSEVLKSQSNKKDIPIVLISDLPEKSLEEMFPSS
                     GANAWMRKPLTRDKMMDMIKRYAR"
     gene            921..1220
                     /locus_tag="VNN25_11245"
     CDS             921..1220
                     /locus_tag="VNN25_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92146.1"
                     /translation="MFAVGAFAPRSGVIVDWFFGKLQEDLTHSFSADVTAVQKQAFAA
                     EMQTMRSAARNGKLKLDSTQKFLKLATEVDGDEKIDHAEADKLIGALRDVNRALK"
     gene            1266..3260
                     /gene="uvrB"
                     /locus_tag="VNN25_11250"
     CDS             1266..3260
                     /gene="uvrB"
                     /locus_tag="VNN25_11250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXH92147.1"
                     /translation="MNFKLISSFTPQGDQPEAIRQLTESVKAGTRDQVLLGVTGSGKT
                     FTVAKVIESVNRPTLVLSHNKTLAAQLYQEFKNFFPENRVEYFVSYYDYYQPEAYIPQ
                     NDLYIEKEMTKNDEIDKLRLAATKALFERRDVIIVSSVSCIYGIGDPDLYHSMLVFFE
                     RGIDKPMTQYLRKLTEMQYERTPYDLSRGTFRVRGDVLELWPAYEDDAVRVEFFGDEI
                     DKMTRIDPITGRTSGQEYDRLAVYPRTHYVTPRDRLMVAMENIRVELDERVVELRANE
                     RLLEAQRLSQRTMFDLEMIAELGYCNGIENYSRHLSGRQPGEPPPTLIDYFPKDLLLV
                     VDESHQTIPQVRAMWGGDRARKQTLVEYGFRLPSAIDNRPLSFDEFEGKINQTLYVSA
                     TPGPFEMEKTGGLFAEQVIRPTGLLDPQISIRPVKGQVDDLIGVIKERIAAGERVMVT
                     TLTKRMSEDLTRYLLELGIKVNYLHSDVETLERIAIIRDFRIGTYDVLVGINLLREGL
                     DIPECSCVAILDADKEGFLRSQTSLIQTIGRCARNLNSLAILYADKMTDSIKFAVAET
                     KRRREIQQQYNEENNIEPASIIKSIDSDLMKMANLDYFEVPGGPTSKARLDLTEGEDL
                     DKAIARLTKEMKDAAKNLDFEKATEIRDKLRELKEIRIFV"
BASE COUNT          694 a         1032 c         1035 g          634 t
ORIGIN      
        1 ttgctctcgg ggcaggagga cgagagctgc tgacttgcga acgcggccca tgtacggcgc
       61 cgagaactgg ctatcgatct cgacgacata gcgaatccgc tccgcatcga ggcccgcggg
      121 gtacgtgacc ggcttctgcg acgactcgat gtgcagtccg ctcgatgcac gcgccgcctg
      181 ccgctgcttc gagatttcca gtaccttcga gcactctttc ggcacgacgt acagcagcgc
      241 gaccgcgatg atgagcggga ttccagcggt tcgaaggatg cgcgtgccgt cggtcatcgc
      301 gcgtagcgct tgatcatgtc catcatcttg tcgcgcgtga gcggcttgcg catccaggcg
      361 ttggcgccag agctggggaa catctcctcg agcgacttct ccggcagatc ggagatcagt
      421 acgatgggga tgtccttctt gttcgactgc gacttcagga cctcgctcag ccggttgccc
      481 ttgatgctgg ggaggttgac gtcgaggaag atgatgtccg gcgagttgct ggcgagcaca
      541 tggttgaact cgatccaact cacggcggtc aggacggtgc aaccctcgcg ctcgagcagc
      601 gcacggccga gcttcagcac gaggggattg tcatcgagaa tcagaacaca ggcgttagag
      661 agagagctgg acatcgcgcg catgatacat tttccggcgg cgcttgctag gcctagagac
      721 tcaatttgaa cgacatcaca ccgaacgaat tgcaagcgcc tgctttggtt cccgagccgc
      781 cgccggctcc cgcacccaat ccacttccct ttcaacgtcc tgctgactac tacgcagatc
      841 ctgagtcgcg gctccggccg ttgtttccaa gatgggtgcc tttgggatgc gggtgggtct
      901 cgctcgtatt cgtagtcctg ttgttcgccg tcggcgcgtt tgctcctcgc tcaggcgtca
      961 tcgtcgactg gttttttggg aaactacagg aagatttgac ccacagtttc tccgccgatg
     1021 tcacggccgt gcagaaacag gccttcgccg cggagatgca gacgatgcgc tccgcagcac
     1081 gcaacggcaa gctcaagctc gatagcacgc aaaagttcct gaagctggcc accgaggtgg
     1141 atggcgacga aaagatcgac catgcggagg cggacaagct gatcggagcg ctgcgtgacg
     1201 tgaaccgggc attgaaatga ggttgcccgg ttgccgagtt gtcccaccaa gccctccgaa
     1261 tccctatgaa cttcaaactc atctcctcat ttacgccgca gggtgatcag cccgaggcga
     1321 tccggcaact cacggagagc gtcaaggccg gtacgcgcga ccaggtcctc ctcggcgtca
     1381 ccggttccgg caagaccttc acggtcgcga aggtgatcga gtcggtcaac cggcccacgc
     1441 tcgtcctcag ccacaacaag acactggccg cgcagctcta tcaggagttc aagaacttct
     1501 ttcccgagaa ccgcgtcgaa tacttcgtct cgtactacga ctactaccag cccgaggcct
     1561 acatcccgca gaacgatctc tacatcgaga aggagatgac gaagaacgat gagatcgaca
     1621 agctgcggct ggcggcgacg aaggctctct tcgagcgccg cgacgtgatc atcgtctcgt
     1681 ccgtgtcgtg catctacggc atcggcgatc ccgatctcta ccactccatg cttgtcttct
     1741 tcgagcgcgg catcgacaag ccgatgacgc agtacctccg caagctgacg gagatgcagt
     1801 acgagcggac gccgtacgat ctttcgcgcg gcacgttccg cgtgcgcggc gatgtgctcg
     1861 agctctggcc cgcgtacgag gatgacgcgg tgcgcgtgga gttcttcggc gacgagatcg
     1921 acaagatgac tcgtatcgat ccgatcaccg gccgcacctc ggggcaggaa tacgaccggc
     1981 tggccgtcta tccgcgaaca cactacgtca cgccgcgcga ccgtctgatg gtggcgatgg
     2041 agaacatccg tgtcgagctg gacgagcggg tcgtggaact gcgcgccaac gaacggctgc
     2101 tcgaagcgca gcgcttgtcg cagcgcacga tgttcgatct ggagatgatc gcggagctcg
     2161 gctactgcaa cggcatcgag aactattcgc gccacctctc cggccgccag ccgggcgagc
     2221 cgccgccgac gctgatcgac tacttcccga aggacctgct gctcgtcgtc gatgaatccc
     2281 accagacgat tccgcaggtt cgggcgatgt ggggcggcga ccgcgcgcgc aagcagacgc
     2341 tggtcgagta cgggttccgt ctgccgagtg cgatcgacaa tcgcccgctg tcgttcgacg
     2401 agttcgaggg aaagatcaac cagacgctct atgtctccgc aacgcccggt ccgttcgaga
     2461 tggaaaagac gggcgggctg ttcgcggaac aggtgattcg tccgacggga ctgctcgatc
     2521 cgcagatcag catccgcccg gtgaaggggc aggtcgacga tctgatcggc gtgatcaagg
     2581 aacggatcgc cgccggcgag cgtgtgatgg tgacgacgct gacgaagagg atgtccgagg
     2641 atctgacgcg gtacctgctg gagctcggca tcaaggtcaa ctatctgcac tcggatgtcg
     2701 agacgctcga gcgcatcgcc atcatccgcg acttccgcat cggaacctac gatgtgctgg
     2761 tcggcatcaa cctgctgcgc gaagggctcg acatcccgga gtgttcgtgc gtggccatcc
     2821 tcgacgccga caaggaaggg ttcctccgct cgcagacatc gctgatccag acgatcggcc
     2881 gctgcgcccg caacctgaac agcctggcga ttctttacgc cgacaagatg accgactcga
     2941 tcaagttcgc agtcgcggag acgaagcggc gccgcgagat ccagcagcag tacaacgagg
     3001 agaacaatat cgaaccggca tcgatcatca agtcgatcga cagcgacctg atgaagatgg
     3061 ccaacctcga ctacttcgaa gtccccggcg gccccacatc aaaagcccga ctcgacctga
     3121 cggaaggtga ggacctggac aaagcgatcg cccgcctgac gaaggagatg aaggacgcgg
     3181 ccaagaacct cgacttcgaa aaggcgacag agatacgcga caagctgcgg gagttgaaag
     3241 agattcggat cttcgtgtag cgctgtcttg tccgcagatg tcacagatga acgcagattt
     3301 gagacgctct atctgcgttt tatctgcgac atctgcggat cggctcactc cccaggcggt
     3361 ttttgcggcg tcaccggcgg cgtgtacggc tgtaa
//
LOCUS       DATLVW010000374         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_386315, whole genome shotgun sequence.
ACCESSION   DATLVW010000374 DATLVW010000000
VERSION     DATLVW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386315"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VNN25_11255"
     CDS             complement(<1..656)
                     /locus_tag="VNN25_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92148.1"
                     /translation="MRARIFFLLLAIAVPAIAFRARPVSDLRFEDQIPADRVWGGPLA
                     AASDGTDYLILTTHEYTANIYAQVISQGVPYGPSVFIGHGFGGSVVWTGSEYLVAWGN
                     ETGVNLSAVSRRGGLLSGPKLLIAQHGIAGYYPLAVSNGREILVTVETVDAIDAIFGA
                     LVSLNGDPVGNEVKIVDHPCCRESAVTGTRDGFALALFGKETRVLHLDDAARPASPGG
                     T"
     gene            complement(689..1714)
                     /gene="ruvB"
                     /locus_tag="VNN25_11260"
     CDS             complement(689..1714)
                     /gene="ruvB"
                     /locus_tag="VNN25_11260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXH92149.1"
                     /translation="MADDRILSAVPVEDDATVELSLRPRRLREYIGQQKVKANLDISI
                     AAARKRHEPLDHVLLFGPPGLGKTTLANIIANEMDAAVRSTSGPALEKAGDLVATLTN
                     LEKGDTLFIDEIHRLPAPVEEVLYSAMEDFYIDVVIGQGPAARTLKMELPHFTLIGAT
                     TRAGLLSAPLRARFGIVHRLDFYDVESLEIIVRRSSDILNIEVTADGSHEIARRSRGT
                     PRIANRLLRRVRDFAEVKREGKIDVGIAREALEMLEVDEFGLDEIDRKLLTTIIERFS
                     GGPVGVGALSSSIAEDRGTIEDLYEPYLIQIGFLQRTPRGRVASKLAYDHLGYAMGAT
                     RKQADLF"
     gene            complement(1805..>2200)
                     /locus_tag="VNN25_11265"
     CDS             complement(1805..>2200)
                     /locus_tag="VNN25_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HXH92150.1"
                     /translation="ISGKEVEAIGKTPRAVPGADAPGEEVAPAKLEEMVWDQLKTCYD
                     PEIPVNIVDLGLVYLCELEDAEGAKNVKIKMTLTAPGCGMGPVLASDVKMKVESLPGV
                     KQADVEVVFDPMWDRSMMSEAAKLQLGMY"
BASE COUNT          400 a          666 c          727 g          407 t
ORIGIN      
        1 gtcccgcccg ggctcgccgg cctggccgcg tcgtccaaat gaagaacccg cgtttccttg
       61 ccgaacagtg ccagggcgaa gccatctctg gtaccagtga ctgcggactc tctacagcac
      121 ggatggtcaa cgattttgac ctcgttgccc acgggatcgc cgttcaagga gaccagcgcc
      181 ccgaagatag cgtcgattgc gtcgaccgtc tcgacggtga cgaggatttc ccggccgttt
      241 gaaacagcga gcgggtaata tccagcgata ccatgttgcg caatgagcag cttcggccct
      301 gacagcagcc cgccgcgccg tgaaaccgca ctcagattga cacccgtttc gtttccccag
      361 gccacgagat actccgaacc ggtccagacg acggatcctc caaatccatg gccgatgaag
      421 actgacggtc catagggaac accttgcgaa atcacctggg cgtagatgtt cgccgtgtac
      481 tcatgtgtgg tgaggatgag gtagtcggtc ccatcgctgg ctgccgctaa ggggccgccc
      541 cacacgcggt cggctgggat ctggtcctcg aaacgcaggt cggaaacagg ccgtgcgcgg
      601 aaggcgatcg cgggaacggc gatcgcgagc aataagaaga agattcgggc ccgcacgtcc
      661 gatatgttat atcagcatat cgacgcaatt aaaacaaatc cgcctgcttg cgggtcgcgc
      721 ccatcgcgta gccgaggtgg tcgtaggcga gtttactggc cacgcggccg cgcggggtgc
      781 gctgcaggaa gccgatctgg atcaggtagg gctcgtagag gtcttcgatc gtgccgcggt
      841 cttcggcgat cgacgatgag agggcgccga ccccgaccgg gccgccgctg aaacgctcga
      901 tgatcgtcgt caggagcttg cggtcgatct cgtcgaggcc gaattcgtcc acttcgagca
      961 tctccagcgc ctcgcgcgcg atgcccacgt cgatctttcc ctcgcgcttg acctcggcga
     1021 agtcgcgcac gcggcggagg aggcggttgg cgatgcgggg cgtgccgcgc gagcggcgag
     1081 cgatctcgtg cgaaccgtcg gcggtgactt cgatgttcag tatgtccgat gagcggcgaa
     1141 cgatgatctc gagcgactcg acgtcgtaga aatcgaggcg atgcacgata ccgaagcggg
     1201 cgcgcagcgg cgcactgagc agaccggcgc gggtcgtggc gccgatgagc gtgaagtgcg
     1261 gcaactccat cttgagcgtg cgcgcagccg ggccctggcc gatgaccacg tcgatgtaga
     1321 agtcttccat cgccgagtag agaacttcct cgacgggcgc gggcaggcgg tgtatctcgt
     1381 cgatgaaaag ggtgtcgccc ttctcgaggt tggtgagggt ggcgacgaga tcgcccgctt
     1441 tctccagcgc cgggccggat gtcgagcgca ccgcggcgtc catctcgttg gcgatgatgt
     1501 ttgcgagagt ggtcttgccg aggccgggcg gaccgaagag gaggacatgg tcgagcggtt
     1561 cgtggcgttt gcgcgcggca gcgatggaga tatcgaggtt cgccttgacc ttctgctggc
     1621 cgatgtactc gcgcagcctc cgcggccgca gcgacaactc caccgtcgcg tcgtcttcga
     1681 caggaacggc ggaaaggatg cggtcgtcgg ccatcccacg cagtttacat ctgcgaccat
     1741 ctgcgtaatc tgcggataag agcttcatcc gcagatggcg cagatggacg cagatagaag
     1801 gaactcagta catgcccaac tgcagcttgg cggcctcgga catcatgctg cggtcccaca
     1861 tcgggtcgaa gacgacttcg acgtcggctt gcttcacgcc ggggagcgac tccaccttca
     1921 tcttcacgtc cgaggcgagc acggggccca tgccgcagcc gggggcggtg agggtcatct
     1981 tgatcttcac gttcttcgct ccctcggcgt cttcgagctc gcagaggtaa acgaggccga
     2041 ggtcgacgat gttgacgggg atctcggggt cgtagcaggt cttgagctga tcccacacca
     2101 tctcttcgag tttggccggc gcgacttctt caccgggagc gtcggcgccg ggcacggccc
     2161 gcggcgtctt gccgatggcc tcgacctcct taccggagat
//
LOCUS       DATLVW010000375         7023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_305591, whole genome shotgun sequence.
ACCESSION   DATLVW010000375 DATLVW010000000
VERSION     DATLVW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305591"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VNN25_11270"
     CDS             <1..1693
                     /locus_tag="VNN25_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH92151.1"
                     /translation="GVRLDQKRYDEAVALATDAIQRSAAGPPVYDAYWHSHMVRGKAL
                     VALGRLDEARGSYESAIAAIEQSRPNVPAPDEDQQLFLDDKDRPYYEMFRTFIGRDPA
                     EALLWVERARSRTLIEFLTYRHRKSIKSLSAEEQAGETRVEKEMAETNVSLREARAQT
                     SPDAGLIGRLEKKLAELRNRRAVVLRELYERHPELAIARGDIPLPSVDEMRATLAPDE
                     IVAEYVSLPDGAWLIAVTRETAPRIYPIHSDDEKLRALSEALSKSIATRDTRVFARSR
                     TLYDLLLRPAEGLLRGKKTIGIVPDGALRTVPFQMLQRPDGKFLIQQHAVFYAPSLAF
                     LHWRFTHPTAGAERQLLAMANPTVSAATVSKSRQHTRSEDLGSLPEAEEEVRGLQSIY
                     GRAQTTLRIGAAATEKDFKHEASRYRVLHLATHGMYDDRDPMYSHLVLARDAGDDDDG
                     LLEAREIVDLDLHAGLAVLSACETGRMRALGGEGMVGLSWAFLGAGCPETVVSMWPVE
                     SAATARMMIDFHRRLVAGASTPEALRRAEIALMGTRRYHHPRYWAPFVVVGAPPR"
     gene            1752..1916
                     /locus_tag="VNN25_11275"
     CDS             1752..1916
                     /locus_tag="VNN25_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92152.1"
                     /translation="MGVTITHLPMLDPERGRTVSVGTFLHEDAAMPRAETSGSYKVIK
                     IFAEPRTPLR"
     gene            complement(2101..2508)
                     /locus_tag="VNN25_11280"
     CDS             complement(2101..2508)
                     /locus_tag="VNN25_11280"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH92153.1"
                     /translation="MTNEICHAIRAARAAINEAIARRDLDAIAAFLLPTYHVVTARSM
                     QRNGKEDSVRSWADMFKRDGSATHGAVPDEIHVNEDWGMAEEHGRWTGTLMANDGPLE
                     IAGVYAAKWQLDAKGWLLQAEIFTPITITRGGS"
     gene            2781..4034
                     /locus_tag="VNN25_11285"
     CDS             2781..4034
                     /locus_tag="VNN25_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXH92154.1"
                     /translation="MPDKKRLRFLSILGPGLVSGAADDDPSGIATYSIAGAQLGTTML
                     WTAFLTWPLMAAVQMMCARIGMVSGYGLAEALRKKFPRWIVAVAGIALLAANIVNIGA
                     DLAGMADAAQMLTTVHSHVWVFVFGIGISAATVWLRYADIARTLKWLALFLFAYVITA
                     FIIRPNWFAILGDAFHPPAFHGKAAWSTLVAILGTTISPYLFFWQASQEVEEEKNIGR
                     KLTIERIGASDREIVDRKIDVGVGTFFSNLVMFFIIVTTALTLHRHGITNIETSRQAA
                     EALRPLAGPGCFLLYTAGLIGVGLLAIPTLSGSAAYALAETFGWRQGLDEKIDQARAF
                     YAVVLLSGAVGIALDFSGVNAVSALYWSAVINGLLAPFLLLGILLVASDAKIMQGQPS
                     SRLGRATVFVTMLLMFGAGIGMFIF"
     gene            complement(4080..4472)
                     /locus_tag="VNN25_11290"
     CDS             complement(4080..4472)
                     /locus_tag="VNN25_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92155.1"
                     /translation="MHFGEKVLVQLEKDLSNLEKALSNRKKVLSNRKKILSNPKKGLS
                     NRKKILSDPKKDLFNPKKILSDRKKILSNPKKILSDLKKVLSSPEKVLSNRKKVLSNL
                     EKVLSYGEKVLLQLDKILVPPLNVLLGA"
     gene            complement(4551..5222)
                     /locus_tag="VNN25_11295"
     CDS             complement(4551..5222)
                     /locus_tag="VNN25_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92156.1"
                     /translation="MSDVTIAATRLSKSYEGASTVTAVDDVSFDVHAGEVVLISGPNG
                     SGKTTLLAMLGGLLLPSSGTVTLNGRNINSLPPGGLPAFRLRSIGFVFQAFRLLDELT
                     ARENVELVLEMAGARDAAIRAAALLEDAGMTDRADRLARNLSSGEKQCVAIARALAND
                     PAVILADEPTGSLDSRAGEVAMRLLSGAARSRGKAVVIVSHDARIEKHVNRVLRMADG
                     RLGDR"
     gene            complement(5219..6331)
                     /locus_tag="VNN25_11300"
     CDS             complement(5219..6331)
                     /locus_tag="VNN25_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92157.1"
                     /translation="MVPLARRMLFRDRGRLLVTVIGLGSTVSLILFLLAVYDGVRDGT
                     INYVRTAVIDLWVCQKNANNLIKSSSFVQTSRAAEISRIPGVRGAAPLMRIITRARIG
                     QRDTSTLFILAFDPRTRIGEPASIAEGTPHLERHDMILDRSFARKYGLAVGSILRIQE
                     TPFRIAAISEGTNALLAQYAFVRIDDGEELLGIPGIASFILVRLGAGANPQQVIDAIR
                     RLDDRLTVYTRDEFVANNLAEIQTGVLPVFGAIIVFGALVCGLVIALMLYSSVLERRE
                     DYATLKALGASHLYLLRLIVVQSIAGSLSGFACGLIVTAATTPILLQFAPSVTVHYTV
                     SAALIVFAGTIVIGICGAAVPIQLLRRIYPAEVFRA"
     gene            6502..6945
                     /locus_tag="VNN25_11305"
     CDS             6502..6945
                     /locus_tag="VNN25_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92158.1"
                     /translation="MAFDTYVSERMRLHATVRAEIPALVEAALEDSHDAALAFELLPG
                     LTLEERQGLLPHLLGLCSSGRYAGRAKAAILELPHDWLRDNIEASAERLLAESDYLDW
                     VNILEVVSQIDPAVRKRLAERAAAHSDPEIRELGEEHLRTRTGAV"
BASE COUNT         1315 a         2188 c         2145 g         1375 t
ORIGIN      
        1 cggcgtgcgc ctggaccaga aacgctatga cgaggcggtt gcgctggcca ccgacgccat
       61 ccagcgatcg gcggccggcc ctcccgttta cgacgcctac tggcattcgc acatggtccg
      121 cggcaaagcg ctggtcgcgc tcgggcgttt ggacgaggcg cgcgggtcgt atgaaagcgc
      181 catcgccgcc atcgagcaga gccgtccgaa cgttccggcg cccgacgaag accagcaatt
      241 gttcctggac gacaaagaca gaccgtatta cgagatgttc cgcacattta tcgggcgcga
      301 tcccgcggag gcactgctct gggtagagcg cgcgcggtcg cgcacactga tcgagttcct
      361 cacctatcgc catcggaaat cgatcaagtc attgtccgcc gaggaacagg ccggcgagac
      421 acgagtggaa aaagagatgg cggaaacaaa cgtctcgctg cgcgaggcgc gcgcgcagac
      481 gtcgcccgac gctgggctga tcggccggct ggagaagaag ctggccgagc ttcgcaatcg
      541 ccgcgccgtg gtgcttcgcg agctgtacga acgccatccc gagctggcca ttgcgcgcgg
      601 agatattccc ttgccgtcgg tcgatgaaat gcgcgcaacc ctcgcgcctg acgagatcgt
      661 cgccgagtac gtctccctcc ctgacggcgc gtggctgatt gcagtcacgc gcgaaaccgc
      721 gccgcgcatt tatccgattc actctgatga cgagaaactt cgagcgctct ccgaggcgtt
      781 gtcgaagtcg atcgccacgc gcgatacccg cgtcttcgcc agatcgcgca cgctctacga
      841 ccttctgctg cgtcccgcgg aagggctgct tcgcggcaag aaaacgatcg gcatcgttcc
      901 cgatggcgca ctgcgcaccg ttccctttca gatgctccag cgcccggacg gcaagtttct
      961 gatccagcag catgccgttt tttatgcgcc gtcgctcgcc ttcctccatt ggcgtttcac
     1021 tcaccccact gccggcgccg aacggcaact cctggccatg gcgaatccga ccgtctccgc
     1081 cgccacggtg agcaagtcgc gccagcacac gcgcagtgag gatctcggct cgttacccga
     1141 ggcggaggag gaagtgcgcg gactgcagtc gatctacggt cgggcacaaa cgacgctgcg
     1201 catcggcgcg gcggccacgg agaaggactt caagcacgag gcgagccgct accgcgttct
     1261 tcatctggcc acgcacggca tgtacgacga cagagatccg atgtactcgc atctcgttct
     1321 ggcccgcgat gccggcgacg atgacgacgg tctgctcgag gcgcgcgaga tcgtggacct
     1381 cgatctccac gccggcctgg ccgtcctctc cgcgtgtgaa acgggacgca tgagggcgct
     1441 cggtggcgag gggatggtcg gcctgtcatg ggccttcctc ggcgcgggct gtccggagac
     1501 cgtagtgagc atgtggccgg tggagtcggc cgccaccgcg cggatgatga ttgacttcca
     1561 ccgccgactc gttgccggag cctcaacgcc cgaggcgctc cgccgcgcgg agatagctct
     1621 gatgggaacc cggcgctacc atcacccgcg ctactgggcg ccattcgtgg tcgtcggcgc
     1681 gccgcctcgg taagccctca gcgtattggg gatgcgcgaa ttaccgtttt tcgcgtgtca
     1741 cggcatgctt cttgggcgtc acgatcacgc acctgccgat gctcgacccg gagcgtggcc
     1801 gcactgtgtc ggtcgggacg ttcctccatg aagacgcggc gatgccgcgc gccgaaacct
     1861 ccggctccta caaggtcatc aagatcttcg cggaaccacg aacaccgcta cggtgaggag
     1921 aattggtttc aatctgcgat gggagaggcc agaatcagcg aatccagcgc gtcctgcgca
     1981 tcgtgcagcg cgttaggagg agcactatgc tgctctcctg agagcgtcca gctcgcgcat
     2041 ccagatgaga atctgtttcc aactcagtgt attttgggac gccaatcgcg gctgctacct
     2101 tcaactgccg ccgcgcgtga tcgtgatggg cgtgaagatc tcggcctgaa ggagccatcc
     2161 cttcgcgtcg agctgccact tcgcggcgta aacgccggcg atctcgagcg ggccgtcgtt
     2221 tgccatcagc gttccggtcc accgcccatg ttcctcggcc atgccccagt cttcgttcac
     2281 gtggatctcg tccggaaccg ctccatgcgt cgcgctccca tcgcgcttga acatgtcggc
     2341 ccaacttctg acgctgtctt ccttcccatt tcgctgcatg ctgcgcgcgg tcacgacatg
     2401 gtacgtggga aggaggaacg cggcgattgc gtccaaatcg cgccgcgcga tggcctcatt
     2461 gatcgcagca cgagcagcac ggatggcgtg acagatctca ttcgtcatcg cggcgatgat
     2521 acgacctcag cggactccgc gctcgacgca aaagcactac ccctgacaag gcatcaccgt
     2581 cgggcaagga tgcctgcact accgcgtcgg aacgtagtgc aggcattcct gcctgcttcc
     2641 gccgggcgtt cctgcccggc ggtgaccgta cgccggagat acccccacga gctctgctct
     2701 cttcatccgc aacgcttgtg gacgtaacgg gtctgatcct ttactttacg gtcgctgcgc
     2761 tcatcctcgg atgatcgatc atgccggaca aaaagcgact gcggtttctc tccatactcg
     2821 ggcccgggct cgtaagcggc gcggccgacg atgatccctc ggggatcgcc acctactcca
     2881 tcgccggagc gcaactcggc acgactatgc tgtggacggc atttctcacg tggccgctca
     2941 tggcggcagt gcagatgatg tgcgcgcgga tcgggatggt ctccgggtat gggcttgccg
     3001 aggcacttcg gaagaagttt ccgcgctgga tcgtcgctgt tgcgggcatc gctctgctgg
     3061 cggcgaacat cgtcaacatc ggggcggacc tcgccggcat ggcggacgcc gcgcagatgc
     3121 tcaccacagt ccactcgcac gtgtgggtgt tcgtgttcgg catcgggatc agcgcggcaa
     3181 ccgtctggct tcgatacgcg gacatcgcga ggacgctgaa atggcttgcc ttgttcctgt
     3241 tcgcgtacgt catcaccgca ttcatcattc gtccgaactg gttcgcaata ctcggagatg
     3301 cgtttcaccc acctgcgttc cacgggaagg ctgcatggtc gacgctcgta gcgatcctcg
     3361 gcacgacgat cagcccttat ttgttcttct ggcaggcatc gcaggaggtg gaggaggaga
     3421 agaacatcgg ccggaagctc accatcgagc gcatcggagc atcggaccgc gagatcgtcg
     3481 accggaaaat cgatgtcggc gtcggaacgt tcttctcgaa tctcgtcatg ttcttcatca
     3541 tcgtgacgac ggcgctcacg cttcaccggc acggcatcac caacatcgag acctcgcgcc
     3601 aggcggcgga agcgctgcgg cccctcgccg gccccggttg cttcctcctc tacacagccg
     3661 gattgatcgg cgtcgggctt ctggcgatcc caaccctctc gggatcggcc gcctatgcgc
     3721 tcgccgagac attcggatgg cgccaggggc ttgacgagaa gatcgatcag gcacgcgcgt
     3781 tctacgccgt cgtcctcctc tccggcgcgg tcggaattgc gctcgacttc tccggcgtga
     3841 atgcggtgag tgcgctctac tggagcgccg tcatcaacgg gcttctcgcg ccgtttcttc
     3901 tcctcggcat cctgctcgtg gcctccgacg cgaagatcat gcaaggccag ccgagctccc
     3961 gcctcggacg cgccacagtc ttcgtaacga tgctcttgat gttcggcgcg ggcatcggaa
     4021 tgttcatttt ctgatgacgc ggcagacggc ggttgcgttc ttccacgaat gagagctgtt
     4081 caggctccga gcaaaacatt tagtggaggg accaaaatct tatctagctg gagcaaaact
     4141 ttctctccgt acgagagaac tttctccagg ttggaaagaa ctttctttcg gttggagaga
     4201 actttctctg ggctggaaag aactttcttt agatcggaaa gaattttctt tgggttggaa
     4261 agaattttct ttcggtcgga gagaattttc tttgggttga aaagatcttt ctttgggtcg
     4321 gaaagaattt tctttcggtt ggaaagacct ttctttgggt tggaaagaat tttctttcgg
     4381 ttggaaagaa ctttctttcg gttggaaaga gccttctcca ggttggagag gtctttctct
     4441 agctggacca gaactttctc tccgaagtgc aaaatctttt acacgccggc tgagatcctt
     4501 cagagtccgg acgaaatcat caactcataa cgggcatgat tagaccccga tcaccgatca
     4561 cccagcctcc cgtccgccat gcgcagtacg cggttgacgt gcttctcgat ccgggcgtcg
     4621 tgactcacga tcaccaccgc cttccctcgc gatcttgcag cgccgctcag aagccgcata
     4681 gccacctcgc ccgcgcggga gtcaaggctg ccggtcggct cgtcggcaag aatcacagct
     4741 ggatcgttag ccagtgcgcg cgcaatggcc acgcactgtt tctcgccgct gctcaggttg
     4801 cgcgccagcc ggtctgcacg atcggtcatg cccgcgtctt cgagcaacgc cgcagcgcga
     4861 atcgcggcat cgcgcgcacc ggccatctcc aggacaagct ccacgttctc gcgtgcggtg
     4921 agctcgtcaa gcaggcgaaa agcctgaaac acgaaaccga tggatcgcaa gcggaacgcg
     4981 gggaggcctc ccggcggcag cgagttgatg ttcctgccat tgagagtgac cgtgccgctc
     5041 gagggcagca gcaagccacc gagcatcgcc agcagtgtgg tcttgccgct cccgttcggg
     5101 ccgctgatca gcaccacctc gccagcatgc acatcgaagg acacgtcgtc gaccgccgtt
     5161 acggtcgaag caccttcata ggacttcgat agacgcgtcg ccgcgatcgt gacgtcgctc
     5221 atgcgcggaa cacctcagcc ggatagatgc ggcgcagaag ctggatcggg acggccgcgc
     5281 cgcagatgcc gatgacgatc gtccctgcga atacgatcaa cgcggcggaa accgtgtaat
     5341 gaaccgtgac tgacggcgcg aactggagga ggatcggcgt cgttgccgcc gtcacgatca
     5401 agccgcaggc aaatccgctg agggagccgg caatcgactg caccacgatc agccgcagca
     5461 aatacaggtg cgaggctccg agcgccttga gggtggcgta gtcctcgcga cgctcgagga
     5521 ctgagctgta gagcatcagc gcaatcacga gcccgcagac gagtgcgccg aatacgatga
     5581 tggccccgaa gaccggcagg acccctgtct gtatttcggc gaggttgttg gcgacgaact
     5641 cgtcgcgtgt gtacacggtc agtctgtcgt cgagccggcg gatcgcgtcg ataacctgct
     5701 gtggattcgc tcccgccccg agtcggacca gaatgaagct ggcgatgccg gggataccga
     5761 ggagctcttc accgtcgtcg atgcggacga aggcgtactg ggcaaggagt gcattggttc
     5821 cttcgctgat ggctgcgatg cggaatggcg tttcctgaat gcgcaggatg gatccaacgg
     5881 cgaggccgta cttccgcgcg aacgagcggt cgagaatcat gtcgtggcgc tcgaggtgcg
     5941 gcgtcccttc cgcgatggaa gccggctcgc caatgcgcgt acgcgggtcg aaggcgagga
     6001 tgaaaagagt agaggtgtcc cgttggccga tacgcgcccg cgtgatgatg cgcatgagtg
     6061 gtgcagcgcc ccgaactccc ggtatccggc tgatctcggc ggcgcgtgaa gtctgcacaa
     6121 acgatgagct cttgatgaga ttgtttgcgt tcttctggca gacccacaag tcaatgacgg
     6181 ccgtgcggac gtagttaatc gttccatcgc gcaccccgtc gtataccgcg agaaggaaga
     6241 ggatgagcga aaccgtcgag cccagcccga ttacagtgac gagtagacgg ccgcgatcac
     6301 ggaacagcat gcgacgggca agcggcacca tggccgtact gtagcagcac ctgggtcgcc
     6361 tccgtttttg gatgccacct agacggatga gcccggaaaa ggccactgga gctactctgt
     6421 ccgacctcct tcgtttacga tttctcgagc atgtgcgctg catcctcaga agcggctctc
     6481 gaagcattcc ggcgcgtcaa agtggcgttt gacacctatg tctccgaacg aatgcgactc
     6541 cacgcgacag tcagagccga gattccggcg ctcgtggaag cggcgctgga ggactcgcat
     6601 gacgcggcgc tcgcgttcga gcttctgccg ggattgaccc tcgaggagcg acagggcctt
     6661 cttcctcatc tcctggggct ttgctcatcc ggccggtacg ccggccgcgc gaaggcggcg
     6721 attctcgagt tgccccacga ttggctgcga gacaatatcg aagcctccgc tgagcggctg
     6781 cttgctgaat cggactacct ggactgggtc aatatcctcg aggtggtttc acagatcgat
     6841 ccggcagtca ggaaacgcct cgccgaacgt gcggccgcgc acagcgatcc ggaaatcagg
     6901 gaacttggcg aagagcacct tcgaacacgt accggcgcag tttgattggc tcgccccgat
     6961 cggttcgtca gtcatcacac cacaaagtca ctgagatcac agagatgaag ctgacctctg
     7021 tgc
//
LOCUS       DATLVW010000376         5180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_511846, whole genome shotgun sequence.
ACCESSION   DATLVW010000376 DATLVW010000000
VERSION     DATLVW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511846"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VNN25_11310"
     CDS             <1..657
                     /locus_tag="VNN25_11310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH92159.1"
                     /translation="EKITLSLDEWKILFLINGRRKLGELALAADEEPLHVYRVIYGLF
                     GNKLIEPVTHAPEPDDTSRTKPAMPALEDTMRQKPATFSADSTVREVADDTNLLVSTE
                     AKLSYKDVVKPTIAQLTITNGEDAGLMFPLTESEYLIGRQRDNQISLADLGVSGHHAR
                     IYRGPDGYSIEDLKSRNGTWLNGARSFHALLKTGDQLRVGATEMRYEILYEGNSVTAV
                     "
     gene            814..1695
                     /locus_tag="VNN25_11315"
     CDS             814..1695
                     /locus_tag="VNN25_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92160.1"
                     /translation="MTRVRLGALLLAFAVSVSAEAAEVLRGDVARSAAIPVILCPTPP
                     RTSLYSGALAFKDYLWVGDAMAFDANGNLYGVDGTSLQSLDTSLQPIRTVKLPEGSSS
                     LAVDAAGFAYVVGKSGIVYVYSAGGAFQRRFALPNLTPPVSAISIDVSPNGCTLVYIG
                     DGGFAYRYDACAQIPLPAIAAGERFQAIRALSDGGIAGAVGNHINFYDATGRLVYELL
                     ASPDSPVLAMAFDAEPNFVWIANETSLFRMHITDHAITAKTSILDPHNVIVFGELRPS
                     AAVLPSLAQAKRRSARP"
     gene            1866..3923
                     /locus_tag="VNN25_11320"
     CDS             1866..3923
                     /locus_tag="VNN25_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXH92161.1"
                     /translation="MHRPMVPLRFLAVLVLLVSVPLFATISYPPPTILTGGSGPSSVA
                     VADFNNDCAADFATADQFANSVTVRFGDGSGGFAASSTITGLVTPMVIVSADFNGDGA
                     PDLAVGNSAGFSADPVGHTITILLNNNDGSGTFTALPPASSPATNGFVTRLVAADVNE
                     DGKIDLLAVDANSSPNGVRYYAGDGTGHFAAAAVISSDTHTPGSLVAVDLNGDHHLDL
                     AVTMGDSTGIFLGNGNGTFGAPTYLPYTDTLNGDGAVHIAAADIDHDGDIDLVTTAGN
                     YPGGVTVFRNNGNATFTRDRFKINSVVTDPIFVALADLDGDGNIDIVTANQFGCTPDG
                     SNLSARPGNGDGTFGAIVPISVGYGPTCINSHWQPNAIAAIDLNCDGAADLIVPNMNT
                     SFTSVLLTAGASDTTAPVVTAPPAVSVPAGPSCNAVVTDAQLGHASATDNCSSCIVIV
                     RTGVPAGNIFPVGTTNVTYTASDGHGNSAAAIQHVTVTDNNPPAITAPADITVSTGAT
                     CSAIVNPGTPVASGPCTGVTVTSVRSDGQPLSAPFPLGTTTITWTATDAANNSASAIQ
                     TITVVAPPLVIGGGTANPSSLWPPTHKMVPVTVSYSTSGGCGGVNCAITSVTSNEPVD
                     GLGDGDMSPDWQIVDAHHLNLRAERSGTGTGRIYTITITCTDGGGHTTMKSVNVTVPM
                     AQN"
     gene            4693..>5180
                     /locus_tag="VNN25_11325"
     CDS             4693..>5180
                     /locus_tag="VNN25_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92162.1"
                     /translation="MDRPTLEQLAQITEPQMVRRRFDEQNLVWGYVISWFFAFTSFFL
                     VGLSLSAKTERSIHIPLSLCDAAFTVFMIAAMTELRRARRRENWKPHAYARFVRANLA
                     GWVIVVIIVKFATLTYFALSGGGWIAWGVVFPIATLALRLLLPQRIALNVMMMAIVLA
                     AL"
BASE COUNT          943 a         1773 c         1492 g          972 t
ORIGIN      
        1 gagaagatca cgctctcgct cgatgagtgg aagatccttt tcctcatcaa cggccggcgc
       61 aagctcggcg agctggcgct tgctgccgac gaagagccgc tgcacgtcta tcgcgtcatc
      121 tacgggctct tcggtaataa gctgatcgag ccggtcacgc atgctccgga accggacgac
      181 acgagccgca cgaagccggc catgccggcg ctcgaagaca cgatgcgcca gaagccggcg
      241 acgttcagtg ccgactcgac ggtgcgcgag gttgccgacg atacgaacct gctcgtctcg
      301 accgaggcga agctctcgta caaagacgtg gtcaagccga cgatcgcgca gctcacgatc
      361 acgaacggcg aagatgccgg cctgatgttt ccgctgacgg aatcggagta tctgatcgga
      421 cgccagcgcg acaaccagat ctccctcgcc gacctcggcg tctccggcca tcacgcgcgc
      481 atctaccgcg gtcccgacgg atactcgatc gaagacctca agagccgcaa tggcacctgg
      541 ctcaacggcg cccgctcgtt ccacgcgctg ctcaaaaccg gcgaccagct ccgcgtcggc
      601 gccaccgaga tgcgctacga gatcctctac gaggggaact cggtgacggc ggtttgagcg
      661 gccgcaccaa aaccctttca ccacggagac acagaggaca cagaggtttc atttcttctc
      721 tgtgatctct gtgtctctgt ggttcatgat tctcaatcgc aattttgacc ggcggacgtt
      781 gcacctccgc gctcacggac ttagacttat ttcatgacgc gcgttcgcct cggcgccctg
      841 ctcctcgctt tcgccgtttc ggtgagcgct gaagcagcgg aggtgctgcg cggcgatgtc
      901 gcgaggagtg cggcgattcc ggtcatcttg tgcccgacgc cgccgcgcac ttccctttac
      961 tcgggcgcgc tcgcgttcaa ggattacctc tgggtcggcg acgcgatggc gttcgacgca
     1021 aacggaaacc tgtacggcgt cgatggaacc tcgctccagt cactggatac ttctctgcag
     1081 ccgatcagga cggtgaagtt gcccgaggga tcgagctcgc tggcggtgga tgccgctggg
     1141 ttcgcgtatg tcgtcggaaa gagcggcatc gtgtatgtgt actccgccgg cggcgcgttt
     1201 caacgacgct tcgctctgcc gaatctcacc ccgccggtca gcgcgatcag tatcgacgtc
     1261 agcccgaacg gatgcacgct cgtctacatc ggcgatggcg gtttcgccta ccgctacgat
     1321 gcctgcgcgc agataccact tcctgcgatt gcggccggcg agcgcttcca agccattcgt
     1381 gcgctgagcg atggcggcat cgcgggcgcg gtaggcaatc acatcaactt ctacgacgcg
     1441 accgggcgac tcgtgtacga gctgcttgca tcgcctgact cgccggtctt agcgatggca
     1501 ttcgacgccg agcccaactt cgtgtggatc gccaacgaaa cgtcgctctt ccgcatgcac
     1561 atcaccgatc acgccatcac ggcgaagacg tcgatcctgg atccgcacaa cgtcatcgtc
     1621 ttcggcgagc tccgtccgag cgctgctgtg ttgccgtctc tcgcgcaggc gaaacgacgc
     1681 tccgcgcggc cttaacgccg gcggcgtaag tcgcgcactt cgcatttcag cgaagggatc
     1741 ccgcctttcc aaaaacgtgc gtcagctcac atccgcacgc tcccatcgaa cttacatttc
     1801 tgctctctct catcgacacc acccttccgc cgattcgcgg gagcggtcaa caataaggag
     1861 ccaccatgca tcgtccgatg gtcccgctgc gtttcctcgc tgttcttgtt ctgctcgtat
     1921 ctgtacctct ctttgccacg atctcctatc ccccgccaac gatcctgact ggcggttccg
     1981 gtccgtcctc cgtcgcggtc gccgacttca acaacgactg cgcggccgac tttgccaccg
     2041 ccgaccagtt cgcgaacagc gtcacggtcc gcttcggaga cggcagcggc ggattcgcgg
     2101 cgtcgtcgac gatcaccggc ctggtcacgc cgatggtcat cgtgtccgcc gacttcaatg
     2161 gcgacggcgc gcccgatctt gctgtcggga acagcgccgg attcagcgct gacccggttg
     2221 ggcacacgat aacgatcttg ttgaacaaca acgacggaag cggcacgttc accgctcttc
     2281 ctccggccag cagtccggcc acaaacggtt tcgtgacgcg gctcgtcgcc gccgacgtga
     2341 atgaagatgg caagatcgat cttcttgcag tcgacgccaa cagctcgccc aacggcgtgc
     2401 gctattacgc cggcgacggc accggacatt tcgccgccgc ggcggtcatc tcgtccgaca
     2461 ctcatacacc cggctcactc gttgctgtcg atctgaacgg cgatcatcat ctcgatctgg
     2521 ccgtcacaat gggcgacagc acaggcatct tcctcggcaa cgggaacggc acgttcggag
     2581 cgccaaccta tcttccctac accgatacgc tcaacggcga cggcgcggtg cacatcgccg
     2641 cggccgacat cgatcacgat ggcgacatcg atctggtcac aacagccggc aactatcccg
     2701 gcggcgtcac cgtcttccgc aacaacggca acgccacgtt cacgcgcgat cgcttcaaga
     2761 tcaattccgt cgtcaccgat ccgatcttcg tggcgctggc ggatctcgac ggcgacggca
     2821 acatcgacat cgtcaccgcg aatcagttcg gctgcacgcc cgatggcagc aacctgagcg
     2881 cgcggcccgg caacggcgac ggaacgttcg gcgccatcgt tcccatcagc gtcggctacg
     2941 gtccgacgtg cattaactcg cactggcagc cgaacgcaat cgccgccatc gatctcaatt
     3001 gcgacggcgc cgcggacctg atcgtcccga acatgaacac atccttcacc agcgtgctgc
     3061 ttaccgccgg agcgtccgat acgaccgcac ccgtggtgac cgcaccgcct gcggtcagcg
     3121 ttcctgccgg accatcctgc aacgccgtcg tcaccgacgc ccaactcggc cacgcctcgg
     3181 cgaccgacaa ctgcagctcc tgcatcgtga tcgtccgcac cggcgttccg gcaggcaaca
     3241 tcttcccggt gggaacgacg aacgtcacct acaccgcgag cgacggccac ggcaacagcg
     3301 ccgccgccat ccagcacgtg accgtcaccg acaacaatcc gcccgcgatc accgcgcctg
     3361 ccgacatcac cgtcagcacc ggcgcgacgt gcagcgccat cgtcaatccc ggcacgccgg
     3421 tcgcctcggg tccgtgcacc ggcgtcacgg tcacgagtgt gcgcagcgac gggcagccgc
     3481 tgtccgcgcc gttccccctc ggcaccacca cgatcacctg gacggcgacc gacgccgcga
     3541 acaacagcgc cagcgcgatc cagacgatca ccgtcgtcgc accgccgctc gtgatcggcg
     3601 gcggtacagc caatccgtcg tcactctggc cgccgactca caagatggtc ccagtcaccg
     3661 tcagctacag caccagcggc ggctgcggcg gtgtcaattg cgccatcacc tccgtcacca
     3721 gcaacgagcc ggtcgacgga ctcggcgacg gcgacatgtc tcccgactgg cagatcgtcg
     3781 atgcgcatca cctcaacctg cgcgccgagc gttccggcac cgggaccggc cgcatttaca
     3841 cgatcacgat cacctgcacg gacggcggcg gccatacgac catgaagagc gtgaacgtga
     3901 ctgtgccgat ggcgcagaat taggttcgtc taagttctct tgccctctcg ccacgctgta
     3961 aattcgagcg ccttctcccc cacgcttttt gtgggggaga aggtgccgaa ggcggatgag
     4021 ggggcgttgc gaccagcact gcatctgtgc atgtgccgcg ttttcaggca gttcggacga
     4081 ggtgtagttg atcaccctgc gtatttgctc ccccctcatc cggcttcgcc accttctccc
     4141 cccgcaaaaa gcgcgggggg agaaggcgct cgatgggaga gcactccaaa gaggagggac
     4201 gaggtgtggc gggaaggtgc aagcgtgtga gaaatgcggg ttgatctcga tctcaccatc
     4261 acctgcacgg gcgacggcgg ccatgcttct tacactttca gcgactcctt tcaatcgagc
     4321 gccttctccc ccacgctttt tgtgggggag aaggtgccga aggcggatga gggggcgttg
     4381 caaccagcac tgcatctgtg catgtgccac gttttcaggc agttcggacg aggtgtagcg
     4441 gatcaccctg cgcatttgct cccccctcat ccggcttcgc caccttctcc ccccgcaaaa
     4501 agcgccgggg agaaggcgct cgatgggaga gcactccaaa gaagagggac gaggtgtggc
     4561 gggaaggtgc aagcgtgtga gaaatgcggg ttgatctcgc tcgcatgacc gcatgctggc
     4621 acccctgcta taccggtccc ccctaagaac cgcggcggca attcgtcgta atcatcggcg
     4681 acaatgctgc cgatggatcg tccaactctc gaacaactgg cacagatcac cgagccgcag
     4741 atggtgcgtc gccggttcga cgagcagaat ctcgtctggg gctacgtgat ctcctggttc
     4801 ttcgccttca cgtccttctt ccttgtcggg ctgtctctct cggcgaagac tgagcggtcc
     4861 atacacatcc ccctctcact gtgcgatgcc gcgttcaccg tcttcatgat cgcggcgatg
     4921 accgaactac gtcgcgcgcg tcgtcgcgag aactggaagc ctcacgccta tgcgcgattc
     4981 gtccgcgcca atctggccgg ttgggtgatc gtcgtgatca tcgtcaagtt cgccacgctc
     5041 acctactttg cattgtccgg cggcggatgg atcgcctggg gtgtcgtctt tcccatcgca
     5101 acgctggcgc tgcggttgct gctgccgcag cgcatcgccc tcaacgtgat gatgatggcc
     5161 atcgtccttg cggcgctctt
//
LOCUS       DATLVW010000377         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_594004, whole genome shotgun sequence.
ACCESSION   DATLVW010000377 DATLVW010000000
VERSION     DATLVW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594004"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /gene="mutS"
                     /locus_tag="VNN25_11330"
     CDS             complement(<1..1809)
                     /gene="mutS"
                     /locus_tag="VNN25_11330"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXH92163.1"
                     /translation="MQQYREIKSRHQDAILFFRMGDFYEMFYEDAEVASRTLGLTLTS
                     RNNGGASAVPLAGVPVKAAGEYLRRLIQHGFRVSICEQTEDPKFAKGIVKREVVETIT
                     PGAAFSDDLLDGTRNNYLCALNRDGDLVGIAAADLSTGELRLTAIGIRDLESALSRFT
                     PREVIISRGESLTQALRGPIGEEGPMVTEREAWEFDSELGRDQLAERFRVSSLEAFGI
                     QDEDGAAIGAAGALLRYLDELQPSGMSHLARPKFERPGGILPLDEMTRRNLELVESMR
                     GAETSGTLLGVLDRTLTPMGARLLRQWLLAPLVNRADIDARLGAVDGLGDGLIRAAMR
                     EALDGVRDIERLGGKAAAGRASPRELRALGDSIARLPAVKSALERARAAITSVDDAAF
                     GRHADAWDGCVDLSDEIIQTLVDRPPIAIGDESSIRSGFDSKLDEWRVLRDGGKDAIA
                     RIQADERTRTGINSLKVGYNKIFGYFIEVTNTNAHLVPSDYQRRQTLAGAERYVTPSL
                     KEYEERVLTAAEKVEERERELFESLRSRVGAQVSRLQVVAQVVAELDVFASLAEAASR
                     EGYVRPQLTDDFALEITAGRHPVVERMMAREKFIPND"
     gene            complement(1833..>2166)
                     /locus_tag="VNN25_11335"
     CDS             complement(1833..>2166)
                     /locus_tag="VNN25_11335"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXH92164.1"
                     /translation="PTDANDSKPSIVDPSKAPKPVSDKPEAKRNTPASRGSGYSVQVA
                     AYTHKAEADKLASSLDKRGYSARVDGSVAPFRVRIGRYATERDAEDALKKLKSKHMDG
                     FVVRAPER"
BASE COUNT          391 a          690 c          609 g          476 t
ORIGIN      
        1 gtcgttcgga atgaacttct cgcgcgccat cattctctct accactggat ggcggccagc
       61 ggttatctcg agcgcgaaat catcggtaag ctggggtcga acgtagccct ctctcgacgc
      121 cgcttccgcc agcgacgcga aaacgtcgag ctctgccacg acttgcgcga ccacctgaag
      181 acgagaaacc tgggctccca cccgtgatcg cagtgactcg aacagctcac gctctcgctc
      241 ttctactttt tcggccgcag tcagtacccg ctcttcgtat tccttgagcg acggcgtcac
      301 ataccgctcc gcgccggcaa gagtctgcct tcgctggtag tcgctcggca ccagatgcgc
      361 gttggtgttc gttacctcga tgaaatagcc gaatatcttg ttgtagccga ccttgagaga
      421 gttgatgccg gtccgcgtgc gctcgtctgc ctgaatcctc gcaatcgcgt ccttgccgcc
      481 atcacgcagg accctccact cgtcgagctt gctgtcgaag ccactgcgta tgctggactc
      541 gtccccaatc gcaatcggtg gcctgtcgac gagagtctgg attatttcgt cggagagatc
      601 cacgcagcca tcccatgcat cggcatgcct gccaaaagcc gcatcgtcta cagacgtgat
      661 agccgcacgt gcgcgttcca gcgctgattt taccgctggc agtcgcgcga tcgaatcgcc
      721 gagcgcgcgg agttcacgtg gtgatgcacg accggctgct gccttacccc ccaaccgctc
      781 aatgtctcgt acgccatcca gggcttctcg catcgcggcc cggatgagcc cgtctccgag
      841 tccatccaca gcgccgagtc tcgcatcgat gtcggcacga ttgacgagtg gcgcgagaag
      901 ccattggcga agcaaacgag cacccatcgg agttagggtg cgatccagaa ctcccaggag
      961 tgttcccgag gtctcggcgc cgcgcatcga ctcgaccagc tcgagattgc gccgcgtcat
     1021 ttcgtcgagg ggcagaatcc cgcctggtct ctcgaacttt gggcgagcca ggtgcgacat
     1081 gccggagggc tgaagctcgt cgagatatcg cagcagtgcc cccgccgcgc ctatcgctgc
     1141 gccgtcttca tcctggattc caaacgcctc gagcgagctc actcgaaatc tctccgccag
     1201 ctgatcgcgt cccagttcgc tgtcgaattc ccaggcttcg cgctctgtaa ccatcgggcc
     1261 ttcctcgccg atgggtcccc ttagagcctg agtgagactc tcgccacgtg aaatgattac
     1321 ttcacgggga gtgaatctcg aaagagcgga ctcgagatca cggattccta ttgcggtcag
     1381 cctgagctcg ccagtagaaa ggtcggcagc cgcgatcccc accaggtcgc catcccggtt
     1441 gagcgcacat agatagttgt tgcgagtgcc gtcgagcagg tcgtcggaga acgccgcgcc
     1501 gggagtgatc gtctccacca cctcacgctt tacgatgcct ttggcaaact tcggatcctc
     1561 ggtttgctcg caaatcgaga cgcgaaagcc atgctggatc agccggcgca ggtattcacc
     1621 ggcggctttg accggaactc ccgcgagcgg caccgcgctc gcgcctccgt tgttcctcga
     1681 tgtgagggtg aggccgagcg ttctcgaggc tacctctgca tcctcataga acatttcgta
     1741 gaaatcaccc atccggaaga acagaatcgc gtcctgatga cgcgatttga tctcccggta
     1801 ctgttgcatc aacggcgttg atgctgtgcg actcaccgtt caggtgctcg tactacgaaa
     1861 ccatccatgt gctttgactt gagtttcttg agtgcgtcct ctgcatcgcg ctcggtcgcg
     1921 taccgtccga ttctgacccg gaaaggtgcg accgatccgt cgactcgcgc tgagtagccg
     1981 cgcttgtcca ggcttgatgc aagtttatca gcctcggctt tgtgggtgta ggcagcaacc
     2041 tgcaccgagt agccacttcc ccgtgaggcc ggagtatttc gctttgcctc gggtttgtcc
     2101 gataccggct tcggcgcttt cgacggatcg acgatgcttg gttttgaatc attcgcgtcg
     2161 gtgggt
//
LOCUS       DATLVW010000378         6685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_551133, whole genome shotgun sequence.
ACCESSION   DATLVW010000378 DATLVW010000000
VERSION     DATLVW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551133"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VNN25_11340"
     CDS             <1..239
                     /locus_tag="VNN25_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92165.1"
                     /translation="DVRVSKDFKFFHNYGVEVIGEIFNLLNAKNPANFNGNCGVDASG
                     KCTFPAGFGQATTFAGDPGQGEQRLAQLGVRITF"
     gene            558..2201
                     /locus_tag="VNN25_11345"
     CDS             558..2201
                     /locus_tag="VNN25_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH92166.1"
                     /translation="MKSFIASVLVLCAATLSAQVSETIDVRIINVDVTVTSKNGPVRG
                     LTRDDFEIFEDGKLQPIKNFYASEATRTTTVPAAPGVGSSAPAAVEQQDPRFQRKVLV
                     LVDSSHTVRYYRDRALRELESMITDRFHGDYQWSIGVIGRGIVLVLPLSSDKQAIHKA
                     LESIRMSGTRKEGTTNFAEGAAEREGGNLVSNLNRSRASDPNAAEQLGEEQLERAESI
                     DDSQRVIAAKLTVPAYLDAVRGFASTSGRKIVLLLTGDPGLNDLEIDRHEPGKGEFTS
                     IRAVGPSATKLWQNQRTIEDLRRLIVQEANASDTSFYMWNVDGLIPAGDMGAAPRPMT
                     NTSALFWLAKETGGRLVTGNDAARAVQEFDTASSTFYSLGYSPAHADDGKYHAISVRM
                     KRKGDYALEYRTGYRDTSTAVQLERAMTSPTAAAMQESALPVTLAFGAPQTVSNEVTV
                     PIEVAVPFRTLQFLPGKNGVAANVVVYVSVFDDMGKNVAASSFALTPGFKSGTPDPNG
                     TLVYRNAIKLRGGERHRVVVAIRDTVTDSVGMATQLVRF"
     gene            2330..2647
                     /gene="rplU"
                     /locus_tag="VNN25_11350"
     CDS             2330..2647
                     /gene="rplU"
                     /locus_tag="VNN25_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXH92167.1"
                     /translation="MYAIIRSGGKQYRVAQGETLYVERDVLSNADQEKITFSEVLMVG
                     GDQPKVGVPMVSGASVMATVVREMRGDKIIVFKKKRRKNHTKRKHGHRQDLVQLRIDS
                     INL"
     gene            2659..2931
                     /gene="rpmA"
                     /locus_tag="VNN25_11355"
     CDS             2659..2931
                     /gene="rpmA"
                     /locus_tag="VNN25_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXH92168.1"
                     /translation="MAHKKGQGSSRNGRDSQPKMLGVKRFAGQFVTGGSILVRQRGTR
                     FYPGVNVGIGRDHTLFAKIDGIVQFHDKGQRGRYISVTPAVTAAAE"
     gene            3095..4144
                     /gene="obgE"
                     /locus_tag="VNN25_11360"
     CDS             3095..4144
                     /gene="obgE"
                     /locus_tag="VNN25_11360"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXH92169.1"
                     /translation="MFIDQATIKVKAGDGGKGCIAFRREKFVPKGGPSGGDGGKGGSV
                     WIVASQRLNTLYHLQFRREWEAGRGQHGMGSNCSGFDGDDITIDLPIGSVVRDKATGD
                     LVADLAHDGERILVAKGGRGGWGNQHFATATRQAPRFAQDGNAGEERELAIELKLIAD
                     VGLVGLPNAGKSTLISVISAAKPKIADYPFTTLIPNLGVVRADDNETFVVADIPGLIE
                     GAHEGHGLGDRFLRHVERCTVLVHLVDLAANETPELDAEVIARELERYSETLATKPRF
                     ICGSKLDSAVPGNSEKLRAYAMKNGYDYYEISAVAGEGVRDLVRVLARFVRENRAEPE
                     RHEAEEARILQRPAI"
     gene            4141..4875
                     /gene="nadD"
                     /locus_tag="VNN25_11365"
     CDS             4141..4875
                     /gene="nadD"
                     /locus_tag="VNN25_11365"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXH92170.1"
                     /translation="MRIGICGGTFDPFHHGHLEPVLAVREEMQWDRVLYIPAWRQPFK
                     AGRIVAAGSHRFAMAALAIRNHDAFYLSPIELERGGISYSVDTLEALHQQYEGASFDL
                     IIGADNLADLHRWKNSERLFQLARFVVLKRGVGGREWGVGEKPGISPALIATRGVGEE
                     LGVGEKEADLSPTPDSRPATPDSPTLHPPPPPTPRIIFAANATVPVSSTEIRERVWAG
                     QSIDAFVDPLVSRYIHHYGLYKEGQP"
     gene            4872..5267
                     /gene="rsfS"
                     /locus_tag="VNN25_11370"
     CDS             4872..5267
                     /gene="rsfS"
                     /locus_tag="VNN25_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXH92171.1"
                     /translation="MNDPIQNRVRTAVAAALDKKALDLQVLAVGNVTSIADYFILCSA
                     TSERQAQAIADGVVDTLRDEGVRPLLVEGATPGRWILVDYGDFIFHIFTEDCRRFYGL
                     ERLWGDAPNVTAELSEEAAPAHVRRKKTS"
     gene            5242..6171
                     /locus_tag="VNN25_11375"
     CDS             5242..6171
                     /locus_tag="VNN25_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92172.1"
                     /translation="MSAARKHRSPVIRDFVRTKSPSMRALLQTVEKVLDHDVNLLILG
                     ESGSGKDFLAEAIHACGARRQNPFIRINCASIPVELFESELFGFEKGTFTDAQARKLG
                     KLELVKGGTIYFDDIASLAPALQAKLLRAIQEKRFTRLGSNQTVALDGRVISSSSVPA
                     EELVNELRRDLFYRINVVTLTIPPLRERREDIPQLARSFLARRKSAIDPVAMRILVEH
                     DWPGNVRELRNVIDRAVLVEESDVLTPSSLPTLTSDFVAAATSHRWTLDELEARYIRD
                     VVRQTKSNYSRAAQILGINRKTLLEKRKRYGIE"
     gene            6326..>6685
                     /locus_tag="VNN25_11380"
     CDS             6326..>6685
                     /locus_tag="VNN25_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXH92173.1"
                     /translation="MKFHLIWPRSNADAEFAAVVERYFNRIKHFFPIEIVEVTSKRGR
                     KSENDVAIMRADSARLLAAIPEQGYVVVLDERGRSVDSLKFASWLERLTIDQPHGVTF
                     VMGGDVGIDESVRRRADY"
BASE COUNT         1322 a         2075 c         2014 g         1274 t
ORIGIN      
        1 tcgacgtccg agtgtcgaag gacttcaagt tcttccacaa ctacggcgtg gaagtgatcg
       61 gcgagatttt caatcttctc aacgcgaaga atccggcgaa cttcaacggc aactgcggcg
      121 tcgatgcttc aggaaagtgc accttccccg ccggttttgg tcaagcaacc acattcgccg
      181 gcgatccagg acagggcgag cagcgcctgg cgcagctcgg tgtccgaatc accttctgat
      241 cgcgacttca tcgtctgatg actacggggc gccgcaaggc gtcccgtagt gttttaggga
      301 cagcgtgtcc ccgccggatg agcaccaggg tcctggccat ggcgtaactc gtttgaagca
      361 aagggggaac atgcttttcg tcgtggcaca tcagcggcat gacgggacga cggagaggga
      421 accgatgtag agagtccatt cggtactcgt actcgtcttc gtggcggcgt ccgtgttcgc
      481 gcagtctctc agcaggaaga tcgaagtctc aggaagccac gccagccttc gtttttgtga
      541 ttctcgagga gcagtccatg aaatcgttca tcgcgtccgt gctggtcctc tgcgcagcta
      601 ctctttctgc gcaggtctcg gaaacgatcg acgtccgcat catcaacgtc gatgtcaccg
      661 tcacatcgaa gaatggccct gttcgagggt tgacgcgcga cgacttcgag atcttcgaag
      721 acggaaagct gcagccgatc aagaacttct acgcgtctga agcgacgcgt acgactaccg
      781 tgccggccgc gccgggcgtg ggttcctctg caccggctgc agtcgaacag caggacccgc
      841 gcttccagcg caaggtcctc gttctggtcg atagcagcca caccgtgcgc tactaccgtg
      901 atcgagctct gcgcgagctc gaatcgatga tcacggaccg cttccacggc gactatcagt
      961 ggtcgatcgg cgtgattggc cgcggcatcg ttctcgtact gccgctttcg tccgacaagc
     1021 aggccatcca caaagcgctg gagagcatcc gcatgagcgg aacgcgaaag gaagggacca
     1081 ccaattttgc cgagggcgcc gcggaacggg aaggcggcaa tctggtctcg aacctcaacc
     1141 gctccagagc gtccgatccg aacgctgcgg aacagttggg agaggagcag ttggagcggg
     1201 ccgaatcaat agacgattcc caaagagtga ttgccgctaa gttgacggtg ccggcgtatc
     1261 tcgatgccgt gcgcggcttc gccagcacct cggggcggaa gatcgtgctg ctcctgaccg
     1321 gcgatcccgg actcaacgac ctcgaaatcg accggcacga gcccggcaaa ggcgaattca
     1381 cgagcattcg cgccgtcgga ccgagtgcca cgaagctctg gcagaatcag cgcacgatcg
     1441 aagacttacg ccgattgatc gtgcaggaag ccaacgcatc cgataccagc ttctacatgt
     1501 ggaacgtgga tggactgatc ccggcgggag acatgggtgc cgcaccccgg cccatgacca
     1561 atacttcggc cctgttctgg ctggccaaag agactggcgg gaggctggta acgggtaacg
     1621 atgctgcccg cgccgtacag gagttcgata cggcctcgtc gaccttctac tcgctcggct
     1681 attcgccagc acatgcggac gatggcaagt accacgcgat cagcgtgcgc atgaagcgga
     1741 agggcgacta cgcccttgaa taccggactg gctatcgcga cacctcgaca gcggtacaac
     1801 tcgagcgagc catgacctca ccaaccgcgg cggcgatgca ggagagtgcg ctgccggtga
     1861 cgctggcctt cggtgcgccg cagaccgtca gcaacgaagt caccgtgccg atcgaagtgg
     1921 ccgtgccgtt ccgcacgctg cagttcctcc cgggaaagaa cggcgtggca gcgaacgtcg
     1981 tcgtctatgt ctcggtcttc gacgacatgg gcaagaacgt tgcggcttct tcgtttgcgc
     2041 tgactccggg gttcaagtca gggactcccg atccgaatgg aacgcttgtc taccgcaacg
     2101 ccatcaaact ccgcggaggc gagcgccatc gggtcgtcgt cgccattcgc gataccgtta
     2161 cggacagcgt cggaatggct acccagttgg tgaggttctg agcactgccg gcgaggaata
     2221 aggcctcgct ccttcgccgt tcaccccagt ccacgccgcc ggaccgtcaa aaattgaccg
     2281 tctgaccgct gtgtgctaga aagcgtggtc tttttcctgg aggtcattcg tgtacgccat
     2341 cattcgttcc ggtggaaagc agtatcgggt cgcgcagggc gagaccctct acgtcgagcg
     2401 cgacgtgctc agcaatgctg atcaggaaaa gatcacgttc agcgaagtcc tgatggtcgg
     2461 cggcgatcag ccgaaggtcg gcgttccgat ggtcagcggc gcctcggtca tggccaccgt
     2521 cgtccgcgag atgcgcggcg acaagatcat cgtcttcaag aagaagcgcc gcaagaacca
     2581 cacgaagcgc aagcatggac accgtcagga tctcgtccag cttcgcattg acagcatcaa
     2641 tctctaagga gaccagccat ggctcataaa aaaggacaag gctcctcacg caacggccgc
     2701 gactcgcagc cgaaaatgct cggcgtgaaa cgcttcgccg gacagtttgt caccggtggc
     2761 tcgattctcg tccgccagcg cggaacgcgt ttttatcccg gcgtgaatgt cggcatcggc
     2821 cgcgaccaca cgctcttcgc caagatcgac ggcatcgtcc agttccatga caagggccag
     2881 cgcgggcgct acatcagcgt tacccctgcc gtcaccgccg ctgccgagta atctccctct
     2941 ttgccgttgt gatgacgccg ctcttcggag cggcgttttg ttttggcgca ccgttcgcac
     3001 gacgggtgtc gggggccctg gagtcgggtg tcggagcatc acaggttttg ctccgacccc
     3061 cgactcccga tccccgatac ccccgaaaaa agaaatgttc atcgatcagg caaccatcaa
     3121 ggtcaaggcc ggagacggcg gaaaaggctg tatcgctttc cggcgcgaga agttcgttcc
     3181 gaaaggtggc ccttccggcg gcgacggcgg caagggtggc tcggtctgga tcgtcgcgtc
     3241 tcagcggctc aacacgctct accacctgca gtttcggcgc gagtgggaag caggccgcgg
     3301 ccagcacggc atgggctcga actgctctgg ctttgacgga gacgacatca cgatcgatct
     3361 tccgatcggg tccgtcgttc gcgacaaggc cacgggcgat ctagtcgccg acctcgcgca
     3421 tgacggcgag cgcattctcg tcgccaaggg cggccgtggc gggtggggca atcagcattt
     3481 cgccaccgcc acgcgccagg cgccgcgttt cgcgcaggat ggcaacgccg gcgaagagcg
     3541 cgagctcgcc atcgagctga agttgatcgc tgacgtcggt ctggtcggtc tcccgaacgc
     3601 cggcaaatcg acgctgatct ccgtgatcag cgcggccaaa ccgaagatcg ccgactatcc
     3661 cttcaccacc ctgatcccga acctcggcgt cgtccgcgcg gacgacaacg agaccttcgt
     3721 tgttgccgat atcccaggac tcatcgaggg cgcccacgag ggtcacggcc tcggcgaccg
     3781 ctttcttcgc cacgtcgagc gctgcacggt tctcgttcac ctcgtcgatc tggctgcgaa
     3841 tgagactccc gagttggatg ctgaggtcat cgcgagggag ttggagcgct actccgagac
     3901 tctggcaacg aaaccgcggt tcatctgtgg atcgaagctc gactccgcgg ttccgggcaa
     3961 ttccgaaaag ctgcgcgcgt acgccatgaa gaacggctac gactattatg aaatctcggc
     4021 cgtcgccggc gagggtgtgc gcgacctcgt gcgcgtcctg gctcggtttg tacgcgagaa
     4081 ccgcgccgag cccgagcgtc acgaagcgga ggaagcccga atcctccagc gtcccgcaat
     4141 atgagaatcg gcatctgcgg cggcacgttc gacccctttc atcacggaca cctcgagcct
     4201 gtccttgccg tccgcgagga gatgcagtgg gaccgcgtgc tctacatccc ggcatggcgg
     4261 cagccattca aagccggtcg catcgtcgcg gccggatccc atcgattcgc gatggcggcg
     4321 ctggcaatcc gcaatcacga tgcgttctat ctctcgccga tcgagttgga gcgcggcggc
     4381 atctcgtact ccgtcgacac gctcgaagca ttgcatcaac agtacgaagg cgcctcgttc
     4441 gacttgatta tcggagccga taatctggcc gacctgcatc gttggaagaa ctcggagcgg
     4501 ctatttcagc ttgcgcgatt tgtggttttg aagcggggtg tggggggtag ggagtgggga
     4561 gtgggagaaa agccgggcat ctcgccagct cttatcgcga cgcggggagt gggcgaggag
     4621 ttgggagtgg gagaaaaaga agcggactta tctccgacac ccgactcccg acccgcgaca
     4681 cccgactctc ccaccctcca tcccccacca ccccccaccc cccgcatcat cttcgccgcc
     4741 aatgccactg ttcccgtttc ttctactgag attcgtgaac gcgtgtgggc ggggcaatcg
     4801 atcgatgcgt ttgtcgatcc cctcgtctcg cgttacattc accactacgg cttgtacaaa
     4861 gagggtcaac cttgaacgat cccatccaaa accgcgtgcg taccgcggtc gccgcagcac
     4921 tcgataagaa agctctcgat ctccaggtcc tcgcggtcgg caacgtcacg tcgatcgccg
     4981 actacttcat cctctgttct gcaacttcgg agcgccaggc tcaggccatc gccgacggcg
     5041 tcgtcgatac gctgcgcgac gaaggggtgc gacctctgct cgtcgaagga gcaacgccag
     5101 gacgctggat tctcgtcgat tacggcgatt tcatcttcca catcttcacc gaagactgcc
     5161 gccgcttcta tggactggag cggctctggg gcgatgcccc gaacgtcacc gcggagctca
     5221 gcgaggaagc ggccccggcg catgtccgcc gcaagaaaac atcgtagtcc ggtcatcagg
     5281 gacttcgttc gcacgaagag tccttcgatg cgggcgcttc tccagacggt ggagaaagtc
     5341 ctcgaccacg acgtaaacct gctcatcctc ggcgagtcag gctccggcaa ggacttcctg
     5401 gccgaagcga ttcatgcctg tggcgcacgg cgtcaaaacc cgtttatccg catcaactgc
     5461 gccagtatcc ccgtcgagct ctttgaatcg gagttgttcg gcttcgaaaa aggcacgttc
     5521 accgatgccc aggcgcggaa gctcggcaaa ctcgagttgg tgaagggtgg gacgatttac
     5581 ttcgatgaca ttgcctctct tgctcccgcg ctgcaggcaa agctgctccg cgcgatccag
     5641 gagaaacggt tcacgaggct cggcagcaac cagacggtcg ccctggatgg ccgcgtcatc
     5701 tcgtcgtcga gcgtgccggc cgaggagctg gtgaatgagc ttcggcgcga tcttttttat
     5761 cggatcaatg tggtcacact cacgattccg ccgctgcggg aacggcgcga agacatcccg
     5821 caacttgccc ggtcgtttct ggctcgaagg aagagtgcca tcgaccctgt ggcgatgcgc
     5881 atcctcgtcg agcacgactg gcccggcaat gtccgtgaac ttcgcaacgt gatcgaccgc
     5941 gcggtcctgg tcgaggagag cgacgtgttg acgccctcat ctctgccaac gttgaccagc
     6001 gattttgtcg ccgcagcaac cagccaccgt tggactcttg atgagttgga ggcgcgctac
     6061 atccgcgacg tcgtgcgcca gacgaaatcg aattactccc gcgctgccca gatcctcggc
     6121 atcaaccgga agacgctgct ggaaaaacgg aagaggtatg ggatcgaatg aagggtgtgg
     6181 ggagtggggc gtggggtgtg ggagagacga aacgcatgcg agacagacta accagccctc
     6241 cgagcacctg cccgctctcc cttgacgacg ccgtctcggc ggtttccacc cccacgcccc
     6301 accctccaca ccccaccccc caaccatgaa atttcacctc atctggcctc gttcgaatgc
     6361 cgacgccgag tttgccgcgg tcgtcgagcg ttacttcaac cggatcaagc atttcttccc
     6421 gatcgagatc gttgaagtga cctcgaagcg cggtagaaag agcgagaatg atgtggctat
     6481 catgcgcgcc gattcggcgc ggctgctggc cgcgattcct gaacaggggt atgtggtcgt
     6541 tctcgatgag cgaggccggt cagtcgattc tttgaagttc gcatcgtggc tggaacgtct
     6601 cacgattgac cagccacatg gcgtcacatt tgtaatggga ggggacgtcg gaatcgatga
     6661 gtccgtccgg cgccgtgccg actac
//
LOCUS       DATLVW010000379        11241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_98291, whole genome shotgun sequence.
ACCESSION   DATLVW010000379 DATLVW010000000
VERSION     DATLVW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98291"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="gcvPA"
                     /locus_tag="VNN25_11385"
     CDS             complement(<1..1301)
                     /gene="gcvPA"
                     /locus_tag="VNN25_11385"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HXH92174.1"
                     /translation="MTNLPDPLTSVHRYISNASEDVREMLEVIGVDSIDRLFETIPDE
                     VKLTALLDIPGPWSEIESRRWFRDLASKNKSAADHVSFLGGGAYLHYQPACIDQLLLR
                     GEFLTSYTPYQPEVSQGTLQTIFEYQTHQCLLTGLDVANASLYDGSTGLCEAVLLAER
                     VVRNRKKVVIARSVHPQYTETVRTYVQNLGYEIVEVGWTADGRVDTAALREACTSDVF
                     AVAVQSPNFLGVIEDYDAIREIADAAGAVKIAVITEATSLGILTPPGAHGFDIAVGEG
                     QAWGIPTQFGGPYVGFMVVKEALKRHMPGRLVGETVDVDGRRAYVLTLATREQHIRRG
                     KATSNICTNEALIALGANMYLSLMGKEGLREVGEQCLQKTAYLRKKLNGVGSVSLPFS
                     GPVYNEIVVRTPFPAAQILADLEHEKILGGIPLGPFYEGHE"
     gene            complement(1303..1692)
                     /gene="gcvH"
                     /locus_tag="VNN25_11390"
     CDS             complement(1303..1692)
                     /gene="gcvH"
                     /locus_tag="VNN25_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXH92175.1"
                     /translation="MTPEDSRYAKSHEYINVEGEIGTIGITDYAQKELGDVVFVELPQ
                     VGTQLEAGDELGSIESVKAVSELFAPVGGEVVEINERLAEKPELINTDPYGDGWMVRI
                     KVADPSEVDELLMTAEEYDEYVETESH"
     gene            complement(1702..2799)
                     /gene="gcvT"
                     /locus_tag="VNN25_11395"
     CDS             complement(1702..2799)
                     /gene="gcvT"
                     /locus_tag="VNN25_11395"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXH92176.1"
                     /translation="MSDEVTTLKKTPLNQLEHELGGKMVDFGGWELPVQFSSILEEHA
                     AVREHVGLFDVSHMGELTVKGPDALALLQRATCNDVSKLENGRAQYNGLLYPTAGFVD
                     DILVYQNAADDFFVVVNASNSDKDYEWIADAAKGMDVEVRNVSADYAQLALQGPEAER
                     LLQPMTDVDLAPMKYYRFGTGKVDGVPAIVSRTGYTGEDGFEVYVAPSEGPRIMRKLL
                     DAGAKPCGLGARDTLRLEAKMALYGNDIDQTTTPLEADLGWIVKVEKGDFFGRDVLQR
                     EKAEGPRRKLAGFEMVDRGIGRHGYPVVDGGEEVGVVTSGTHSPTLKKAIGLAYLPLD
                     KSAPGNEFMVLIRGKEVRARVVPTPFYKRSR"
     gene            complement(2971..3693)
                     /locus_tag="VNN25_11400"
     CDS             complement(2971..3693)
                     /locus_tag="VNN25_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HXH92177.1"
                     /translation="MPLMVFLDEAGDHSLDPVDKDFPVFVLVFLLCEQTDYTNVIIPA
                     FTRFKIDHFGHDGAILHSRDIRKALGDFSFLRVPERRELFHADLNRLMRDAPFELIAI
                     AIRKDLHKQRYGSSSRNPYDLALEFGMERLKAYVDELGQRHVTLLAEARGKNEDNALR
                     LSFLQLLKHGSFYQKFDDTEFDLKFVAKKANVLGHQLADLCAYPIARRIITFTKSSRA
                     FDIVRLKFLQRPGWRHGFKIFP"
     gene            3744..4646
                     /gene="xerC"
                     /locus_tag="VNN25_11405"
     CDS             3744..4646
                     /gene="xerC"
                     /locus_tag="VNN25_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXH92178.1"
                     /translation="MKNEIGDFLDYLTYERNVSVNTITAYRDDLESLISFLCNDYLTL
                     GRDQLDFTVVDHLAIRSYLAHLARRKLARASAARHLSAIRSFFKYLMREGVMEANPAR
                     SVATPKREKHLPTVLQPADVALLIEQPDTSNALGLRDRAFLEVLYASGLRISELVGIN
                     IEDVELRARLVKVRGKGSKERIVPFGSKAEASIREWLAVRGELATDIEEPALFVNYRG
                     QRITTRSVRRLFDRYVRRASLRAGVSPHTMRHSFATHLLNAGADLRGIQELLGHVSLS
                     TTQKYTHLNDWQLIAVYKKAHPKA"
     gene            5177..7192
                     /locus_tag="VNN25_11410"
     CDS             5177..7192
                     /locus_tag="VNN25_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXH92179.1"
                     /translation="MANRLANETSPYLLQHAHNPVDWYPWGDEAFARARAEDKPLFLS
                     IGYSACHWCHVMERESFENEELAAILNEKFIAVKVDREERPDVDSIYMEAVQMMTGHG
                     GWPMSMFLTPGGAPFYAGTYFPPDDRHGMPGFRRVLEHVSEAYRSRREDVEAASAEVV
                     RSLSTVKFQGGGALEHDALDRAAARIAQGYDSAHGGFGGAPKFPPSMSLDFLMQVAWR
                     NGDKRLDEIITHTLTKMARGGMYDQVGGGFHRYSTDAHWLVPHFEKMLYDNALLARLY
                     TRAWQYTKDPFFARIASEILGFVQREMTSPDGGFYATQDADSEGVEGKFYVWSRAEVM
                     TLLGEEEGRIFCALYDITDRGNWEGENILNMPRDPESVAADLGITIDHLVDVAARGKC
                     KLYGARSQRVWPARDKKILAGWNGWMLVAFAEAAVAFDKDSYRDVVRHNADFLLTRID
                     TSGRLMRTPKIAGLLEDYSGVAWGLALAYEATHERRYLDAARQLADQILDRFADAQNG
                     GFFDTPTDHEALITRPKDLFDNATPSGNSVAADVLLRLALLFGEERYAEAATKTIAAI
                     APLAERYPSGFGLLLGVAEWRAGQPKEIVITGSIDDPTFRALRKVAGEEYLPHRVLVA
                     GSGSGDLPLMEKRPADRAMAYVCEAYACAEPTVDAERLRVLLQIAKA"
     gene            7202..8476
                     /locus_tag="VNN25_11415"
     CDS             7202..8476
                     /locus_tag="VNN25_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92180.1"
                     /translation="MTVSDSEATAPWPLVKRILFRFAAAFFILVNLPFPFNRIPWAPL
                     TRFLDTMEAAAVRAMARLLHVSVAVLPNGSGDTTYSWVQLLCIAIGAVLITIVWSVID
                     RRRRDYDRAWIALRAYLRFALAIAMMRYGAAKVIPSQFPPPTLDRLLQPFGDASPMGL
                     LWTFMGASAAYTIFAGALEMLGGLLLTMRRAALLGALVSAGVLANIVALNFMYDVPVK
                     IYSLELLAMAVVIALPDVPRLVDFFVRNRPVTTAPLPRFWRNRAVEIAGVVLRTLAVA
                     VVVLTSFQEVRAMQRMYGGAEARSPLYGIWRVDDFQVDGKTIPVNDPARWRRVIFDLR
                     YMASVQLMNDSRQRVNVQLVEKTQKLIVSRRNEPAWDGILSYARPDAETLIIDGDLGG
                     HKVHALCHREPIPKFLLTTRGFHWINEYPFNR"
     gene            8487..9398
                     /locus_tag="VNN25_11420"
     CDS             8487..9398
                     /locus_tag="VNN25_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA U-34 5-methylaminomethyl-2-thiouridine
                     biosynthesis protein"
                     /protein_id="HXH92181.1"
                     /translation="MSGTIIGGVLAPHPPHIVYGDNHWRNEPRSECGWEVLRWGYERA
                     RKHFLEKKPDVFLVHSPHWQTVIGHHFLGIPEFHGLSVDPIFPNLFRFNYDIKVDVEL
                     AELIAEEGRRDGLITKMMRNPNFRVDYGTIVSCHMMNPEWDIPIVGISSNNSPYYFSN
                     EMGQQQMLRLGQATKRAIEKSGRRAVLLASNTLSHRHFTTEPDIPEDMSREHVYEHSG
                     YLWDMKVLDLMRKGRTRELIAVLPEFMDMAVSEVKAGSLAWMLSALDFPSYPAEVYGY
                     WTVIGTGNAVVGWDPQLKAEATTQGAA"
     gene            9395..9862
                     /locus_tag="VNN25_11425"
     CDS             9395..9862
                     /locus_tag="VNN25_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92182.1"
                     /translation="MIAVLALFFATAFPSNSRTSWMRPESFHLTIGMSRAAAVHALES
                     NGWKTKRGDGENQLVVEYADDKSLTLQFERERLKSARFELFTILQDAPKAFDEEWAYL
                     HEALGKPRMKSQKIVLYDQILPNVMAVLSADPKSEQAQKGVGMLVVRYYDPAN"
     gene            10028..11101
                     /locus_tag="VNN25_11430"
     CDS             10028..11101
                     /locus_tag="VNN25_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HXH92183.1"
                     /translation="MTTSDPSREAIKGRGASWNPQNRFEKLEYVLDEDASPSDDEPAP
                     LPRTLFLRDPTRTILAHNDSPDVGFDTSINPYRGCEHGCIYCYARPSHEFLGFSAGLE
                     FETKILVKEDAPELLREELMSRKYEPRMIAISGVTDPYQPIERKLEITRRCLAVLAEF
                     RNPVGIITKNHLVTRDIDILQELAAHNAVSVHISVTTLDPHLANLMEPRASRPELRIA
                     AIEALAKAGIPAGVMVAPVVPAITDHEMPAILTAAANAGGTSAGFVVLRLPWAVAPLF
                     EKWLEEHYPDRKEKVMNRVRDLREGKFYDPQWGVRQRGKGIFADQIASMFEVTCRRLH
                     LNEKKYDLSTDAFRRPTTQGSLF"
BASE COUNT         2063 a         3398 c         3483 g         2297 t
ORIGIN      
        1 ttttcgtggc cttcgtagaa cggcccgagg ggaatcccgc cgaggatctt ttcgtgctcg
       61 aggtcggcga ggatctgggc ggcgggaaac ggtgtccgca cgacgatctc gttgtagacc
      121 ggaccactga acggcaggga aaccgatccg actccattga gtttcttgcg cagatacgcg
      181 gtcttctgca ggcactgctc gccgacttcg cgcagtcctt ctttgcccat cagcgacaga
      241 tacatgttcg cgccgagcgc gatcagcgcc tcattcgtgc agatgttcga tgtggccttg
      301 cctctgcgga tgtgctgctc gcgcgtggcc agcgtaagca cgtatgcgcg gcggccatcg
      361 acgtcgacag tctcgccgac cagacgtccc ggcatgtgcc gcttcagggc ttctttgacg
      421 accatgaagc cgacgtaggg accgccgaac tgcgtcggga tgccccaggc ctgtccctcg
      481 ccgacggcaa tatcgaaccc atgcgcgccg gggggagtaa ggatgccgag ggatgttgct
      541 tcggtgatca cggcgatctt cacggcgccg gcggcgtcgg caatctcgcg aatcgcgtca
      601 tagtcttcga tcacgccgag gaagttcggc gattgcacgg cgaccgcgaa gacgtcgctc
      661 gtacacgcct cgcgaagcgc tgccgtgtcg acgcgtccat ccgcggtcca gcccacttcc
      721 acgatctcgt agccgaggtt ctgcacgtac gtgcggaccg tttcggtgta ctgcggatgc
      781 accgaccgcg cgatcacgac cttcttgcga ttgcgcacga cgcgctccgc caggagcacc
      841 gcctcgcaca atccggtcga gccgtcgtag agcgacgcgt tggcgacatc gagtccggtc
      901 agcagacact gatgcgtctg gtactcgaag atggtttgca gcgtcccctg cgagacttcg
      961 ggctgatacg gcgtgtacga ggtcaggaac tcgccgcgca gcagcagttg atcgatgcac
     1021 gcgggctggt agtgcagata cgcgccgccg ccgaggaacg agacgtgatc ggcagcggac
     1081 ttgttctttg atgcaaggtc gcgaaaccag cgccgcgatt cgatctccga ccaagggccc
     1141 gggatgtcga gcagcgcggt tagtttgacc tcatccggga tcgtctcgaa aagcctgtcg
     1201 atggaatcga cgccgatgac ctcgagcatc tcgcggacat cttcggaagc gttggagatg
     1261 tagcggtgta ccgaggtaag aggatcgggg agattggtca cgttagtggg attccgtctc
     1321 cacgtactcg tcgtactcct cggcggtcat gagcagctcg tcgacctcac tcgggtcggc
     1381 gactttgatg cggaccatcc aaccgtcgcc gtacggatcg gtgttgatca actccggctt
     1441 ctcggcgagg cgctcattga tttcgacgac ttcgccgccg acgggagcga aaagctcgga
     1501 gacggctttg actgattcga tcgatccaag ctcgtcaccg gcctcgagtt gcgtaccgac
     1561 ctgcggcagc tcgacgaata cgacgtcgcc gagctctttc tgcgcgtagt cggtgatccc
     1621 gatggttccg atctcacctt ctacgttgat gtattcgtgg ctcttggcgt atctactgtc
     1681 ttccggagtc atcgccgctc cttatctcga tcgcttgtag aagggtgtgg ggacaacgcg
     1741 cgcccgaact tcttttcctc ggatgaggac catgaattcg ttgccgggcg ctgatttgtc
     1801 gagcggaaga tatgcgagac cgatcgcttt tttcaatgtc ggcgagtgcg taccgcttgt
     1861 gacgacgccg acttcctcgc cgccatcgac gacggggtat ccgtgccggc cgattccgcg
     1921 gtcgaccatc tcgaatccag ccagcttgcg gcgcggtcct tccgccttct cacgctggag
     1981 gacgtcgcgg ccgaagaagt cccccttttc gaccttgacg atccatccga ggtcggcttc
     2041 gagtggggtg gtggtctggt cgatatcgtt tccgtagagc gccatcttcg cttcgagccg
     2101 gagcgtgtcg cgtgcgccga ggccgcatgg cttcgccccg gcgtcgagga gcttgcgcat
     2161 gatgcgggga ccttcggacg gcgcgacgta gacttcgaat ccatcttcgc cggtgtagcc
     2221 ggtgcgcgag acgatggccg gcacgccgtc gaccttgccg gtgccgaagc ggtagtactt
     2281 catcggagcg aggtcgacgt cggtcatcgg ctggagcagg cgttcggcct ccgggccctg
     2341 cagcgccagt tgcgcgtaat cggcgctgac gttgcggacc tcgacgtcca tccctttcgc
     2401 ggcgtcggcg atccattcgt agtctttgtc gctgttcgag gcgttgacga cgacgaagaa
     2461 gtcatcggca gcattctgat agacgaggat gtcgtcgacg aacccggccg tcggatagag
     2521 caaaccgttg tactgcgcgc gcccgttctc gagcttcgag acgtcgttgc acgtcgcgcg
     2581 ctgcagaagc gccagggcat ccggaccttt gacggtcagc tcgcccatgt gcgagacatc
     2641 gaaaaggcca acgtgctcgc gaaccgcggc atgctcctcg aggatgctgc tgaactgcac
     2701 cggaagctcc cagccaccga agtcgaccat cttcccgccg agctcgtgct cgagctggtt
     2761 cagcggggtt ttcttgagag ttgtgacctc gtcagacatg gacagcctcc gggggacgcg
     2821 aacctagcac gcgctgtgcc gcgggcgcaa tgacggtggg acgaagactt gacgacggaa
     2881 tcaggcccgc gtaccgttcg ttgttaatcc tgatttggga ctgggaactc ccggtcggca
     2941 actggccctg agcgatcggg gccttttgtt ttacggaaag atcttgaacc cgtggcgcca
     3001 gcccggcctt tgaagaaact tcagccggac gatatcgaac gcgcggctac tcttcgtgaa
     3061 agtgatgatt cggcgcgcga ttggatacgc gcagaggtcg gccagttgat gaccgagtac
     3121 attcgctttc ttggcgacga atttcagatc gaactcagtg tcatcaaact tctggtagaa
     3181 agaaccgtgc ttcagaagtt gaaggaagga gagacgcaac gcgttgtctt cgttctttcc
     3241 tcgtgcttca gcgagcagcg tgacgtgccg ctgacctagc tcatcgacat acgcttttag
     3301 ccgctccatc ccgaactcga gcgcgaggtc gtaaggattg cgactgctgc ttccatagcg
     3361 ttgcttgtgc aggtctttgc ggatggcgat tgctattagc tcgaaggggg cgtctcgcat
     3421 taggcggttc agatcggcgt gaaataactc tcgacgctcc ggaaccctca ggaaggaaaa
     3481 gtcgcccagc gccttccgaa tgtcgcgcga atggagaatc gctccgtcgt gcccaaagtg
     3541 gtcgatcttg aaacgggtga acgcaggaat gatcacattc gtgtagtcgg tctgctcgca
     3601 caacaggaag acgagaacga aaactgggaa atccttatcg accgggtcga gcgagtgatc
     3661 acccgcctca tcgaggaaca ccatgagagg catggcctat cgtaacttgt cgtggcgtat
     3721 catcgttatc gggagtggcg ggcgtgaaga acgaaattgg cgatttcctc gattacctta
     3781 cctacgagcg gaacgtctcc gtcaacacca tcaccgcata ccgggacgat ctcgagtcgc
     3841 tgatctcgtt tctctgcaac gactacctga cgctgggccg cgatcagctc gatttcacgg
     3901 tcgtcgatca tctggccatc cgctcgtatc tcgcgcatct ggcccgccgc aagctcgcac
     3961 gcgcctcggc tgcccggcac ctgtcggcaa tccgatcgtt cttcaagtac ctgatgcgcg
     4021 aaggtgtgat ggaggccaac cctgcccgca gcgtggccac gccgaagcgc gaaaagcatc
     4081 tgcccactgt gttgcagccg gctgacgtgg cgctcctgat cgagcagccc gacacgtcga
     4141 atgcgctcgg tcttcgcgac cgcgcctttc tcgaggttct ctacgcctcg ggattgcgga
     4201 tcagcgagct agtcggaatc aacatcgagg atgtcgagct gcgtgcgcgg ctggtgaagg
     4261 tgcgcggcaa aggatcgaag gaacggatcg ttccgttcgg ttcgaaagcg gaggcgtcga
     4321 ttcgcgagtg gctggccgta cgcggcgagc tggccacgga catcgaggag ccggctctct
     4381 tcgtcaacta ccgcgggcag cgcatcacga cgcgtagcgt gcgacggttg ttcgaccgct
     4441 acgttcggag agcgtcgctg cgcgcaggcg tgtcacccca cacgatgcgc cactccttcg
     4501 ccacgcatct gctcaacgcc ggagccgatc ttcgcggcat ccaggagctc ctcggccacg
     4561 tctcgctctc gacgacccag aagtacacgc acctaaacga ctggcagttg atcgcggtgt
     4621 acaagaaggc gcacccgaag gcgtgagagc gggtcattcc cgtgttgatt gggatcacat
     4681 ctgagttgcc gcgagtcccg acgttacttt cttgagtccg gacgttcgtt ctcgagtccg
     4741 gaccttcgtt tcttgagttc ggaccttcgt ttcttgagtc cgggtcttag ttcttgagtc
     4801 cggaccttcg tttcttgggt ccagaccttc gttcttgggt ccggaccttc gtttcttgag
     4861 tccggatctt agttcttggg tccggacctt cgttccttga gtccggacct tcgttcttga
     4921 gtccagacct tcatttcttg agtccggacc ttcgtttgct cagtccggac cttcgttctt
     4981 gagtccagac cttcgttttt tgagtccgga ccttcgtttc ttgagtccgg actcaaatct
     5041 tttgagtcga aagcaccgat tttggagtgt atcgtggcaa aacttgccga atgtgacatc
     5101 atctcgactg acttgatgat gtcgatgtca aatttgctga agatcgcggc agacaaggcg
     5161 ggcatagact ctgaacatgg ccaaccgcct cgccaacgag acttctccct acctcctgca
     5221 gcacgcgcac aaccccgtcg actggtatcc gtggggcgac gaggcattcg cgagggcgcg
     5281 cgccgaggac aagccccttt ttctctcgat cggctactcc gcctgccact ggtgccatgt
     5341 gatggagcgc gagtcgttcg aaaacgagga gctggcggcg atcctcaacg agaagttcat
     5401 cgccgtcaaa gtcgaccgcg aggagcggcc tgacgtcgac tcgatctaca tggaagcggt
     5461 gcagatgatg accggccacg gcggctggcc gatgtcgatg ttcctcacgc cgggcggcgc
     5521 gccgttctac gccgggacgt actttccgcc ggacgatcgt cacggcatgc ccggcttccg
     5581 gcgggtgctg gagcatgtat ccgaggcgta tcggtcgcgg cgcgaggatg tggaggccgc
     5641 gtcagccgag gtcgtccgct cgctgagcac ggtgaagttt cagggcggcg gagctctgga
     5701 gcatgacgcg ctcgatcgtg ccgccgcgcg catcgctcag ggctacgact ccgcccacgg
     5761 cggcttcggt ggcgcgccga agttcccgcc gtcgatgtcg ctcgattttc tgatgcaggt
     5821 tgcctggcgc aacggcgaca aacgcctcga cgagatcatc acgcacacct tgacgaagat
     5881 ggctcgcggc gggatgtacg accaggtcgg gggcggcttt catcgttact ccaccgacgc
     5941 gcattggctg gtgccgcact tcgagaagat gctgtacgac aacgcgttgc tggcgcgcct
     6001 ctacacccgg gcgtggcagt acacgaagga tcccttcttc gcccgcatcg ccagcgagat
     6061 cctcggcttc gtgcagcgcg agatgacctc gcccgacggc ggcttctacg cgacgcagga
     6121 cgccgattcg gaaggtgttg aaggcaagtt ctacgtctgg tcgcgcgcgg aggtgatgac
     6181 tctactcggt gaagaggagg gacgcatttt ctgcgcgctc tacgacatca ccgaccgcgg
     6241 caactgggag ggagaaaaca tcctcaacat gccgcgtgat ccggagtcgg tcgctgccga
     6301 tctcggaatc acgatcgatc acctcgtcga cgtcgccgcg cgtggcaagt gcaagttgta
     6361 cggcgcgcga tcgcagcgcg tatggccggc acgcgacaaa aagatcctcg ccggctggaa
     6421 cggctggatg cttgtcgcat tcgccgaggc ggcggtcgcg ttcgataagg actcgtatcg
     6481 cgatgtcgtt cggcacaacg ctgattttct tctgacacgg atcgacacca gcggccggct
     6541 gatgcgcacg ccgaagatcg ccggcctgct cgaggattac agcggcgtcg cctgggggct
     6601 cgcgctcgca tacgaggcta cgcacgagcg gcgttacctc gacgccgccc gccaactggc
     6661 cgatcagatc ctcgatcgtt tcgccgatgc gcagaacggc ggtttcttcg acacgccaac
     6721 cgatcacgag gcgctcatca cgcggccgaa ggatctcttc gacaacgcca cgccctcggg
     6781 gaactccgtc gcagccgacg tcctcctccg gctcgcgctt ctctttggcg aagagaggta
     6841 cgccgaggcg gcgacgaaga cgatcgcggc aatcgcgccg ctcgccgagc gctatccctc
     6901 gggcttcgga ctcctgctgg gagttgccga gtggcgggcc gggcagccga aagagatcgt
     6961 gatcacaggt tcgatcgacg atccgacgtt ccgtgcgttg cgcaaagtcg ctggcgaaga
     7021 gtatctgccg caccgagttc tggtggccgg ctcgggatca ggtgatcttc cgctgatgga
     7081 gaagagacct gccgatcgcg cgatggccta tgtctgcgaa gcgtatgcgt gtgcggagcc
     7141 gaccgtagac gcggagcggc tgcgcgtgtt gcttcaaatc gcgaaggcct aaactgtcgc
     7201 aatgaccgtc agcgattcgg aagccacggc gccctggccg ctggtgaagc gcattctctt
     7261 ccgcttcgcg gcagccttct tcatcctcgt gaatcttccg tttcccttca atcggatccc
     7321 atgggcaccg ctgacgcgct tcctggacac gatggaggct gcggcggttc gggcgatggc
     7381 gcgccttctc cacgtctcgg tcgccgtcct tccgaatggc agcggcgaca cgacctacag
     7441 ctgggttcaa ctgctatgca tcgcaatcgg cgccgtgctg atcacgatcg tctggagcgt
     7501 aatcgatcgg cggcgcagag actacgaccg ggcctggatc gctcttcgcg cgtatctccg
     7561 cttcgcactc gcgatcgcga tgatgcgata cggcgctgcc aaggtgatcc cttcgcaatt
     7621 tccgccgcct actctcgatc gtctgctaca gccgtttggc gacgcctcgc cgatggggct
     7681 tctctggacg ttcatgggag cgtcggcggc ctacaccatt ttcgccggcg cgttggagat
     7741 gctcggcggc ttgctcctga cgatgcgccg tgcggcattg ttgggcgcac tcgtctcggc
     7801 gggcgttctg gccaacatcg tcgcgctcaa cttcatgtac gacgtgccgg tgaaaatcta
     7861 ttcgctcgaa ctgctggcga tggcagtcgt gatcgctctt ccggacgtgc cgcggctcgt
     7921 cgatttcttc gttcgcaacc ggcccgtcac aaccgcgccg ctgccacgct tctggcgaaa
     7981 ccgcgccgtc gagatcgccg gagtcgtttt gcgcacgctc gctgtcgctg tggttgtgct
     8041 gacgtcgttc caggaagtgc gcgcgatgca acggatgtat ggcggagcgg aggcacgctc
     8101 gccgctttac ggaatctggc gcgtcgatga cttccaggtc gacggcaaaa cgattccggt
     8161 taacgatcct gcgcgctggc gccgcgtcat cttcgacctg cgctacatgg cctcggtgca
     8221 gctgatgaac gattcccggc agcgggtgaa tgttcagctc gtcgaaaaga cgcaaaagct
     8281 gatcgtctcg cgaagaaacg agccggcatg ggacggaatc ctttcctatg cgcggccgga
     8341 cgccgaaacg ctgatcatcg acggcgatct tggcggccac aaagtgcacg cgctctgtca
     8401 ccgcgagcca atcccgaagt tcctcctgac gacccgcggc tttcactgga ttaacgagta
     8461 cccgtttaat cgctaaacta tccgcaatgt ccggaaccat catcggcggc gttctcgcgc
     8521 cccatccgcc gcacatcgtt tacggcgaca accactggcg caacgagccg cgctcggagt
     8581 gcggatggga agtgctgcgc tggggatacg agcgggcgcg caagcatttc ctggaaaaga
     8641 agccggacgt ttttctcgtc cactcgcccc actggcagac cgtgatcggc catcacttcc
     8701 tcggtatccc cgagtttcac gggctctcgg tcgatccgat ctttccgaat ctgttccgct
     8761 tcaactacga catcaaagtc gacgtcgagc tggcggaatt gatcgctgaa gaaggtcggc
     8821 gcgacgggtt gattacgaag atgatgcgga accccaactt ccgcgtcgac tacggcacga
     8881 tcgtctcctg ccacatgatg aatccggagt gggacatccc gatcgtcggc atctcctcga
     8941 acaactcacc gtactatttc tcgaacgaga tggggcagca gcagatgctg aggctcggtc
     9001 aggccacgaa gcgggccatc gagaagagcg ggcggcgcgc ggtgttgctg gcgtcgaaca
     9061 cgctttcgca tcgtcatttc acgacggagc ctgacatccc cgaagacatg tcgcgcgagc
     9121 acgtctacga gcacagcggc tatctatggg acatgaaagt gctcgacctg atgcgcaaag
     9181 ggcggacgcg cgagctcatc gcggtcctgc ccgagttcat ggacatggcg gtgagcgagg
     9241 tgaaggcggg atcgcttgcg tggatgctct ccgcgctcga cttcccgtcc tatcccgccg
     9301 aggtctacgg gtattggact gtgatcggga cggggaatgc cgtggttggt tgggatccgc
     9361 aactgaaagc tgaggcaacg acgcagggag cggcatgatc gctgtcctcg cactcttctt
     9421 cgcgaccgcg tttccgtcga acagcaggac ctcgtggatg cgtccggagt cgtttcatct
     9481 gacgatcgga atgagcaggg cagcggctgt gcatgcgctg gagtcgaacg gctggaagac
     9541 gaagcgcggc gatggcgaga atcagctcgt cgtcgagtac gccgacgata agtcgctgac
     9601 gttgcagttc gagagagagc ggttgaagtc cgctcgcttc gagctcttca cgattttaca
     9661 ggacgcgccg aaagcgttcg atgaagagtg ggcctacctg catgaagcgc tgggaaagcc
     9721 gcgcatgaag tcgcagaaga tcgtgctcta cgaccagatc ctgccgaacg tgatggccgt
     9781 cctttccgcc gatccgaaat cggaacaagc gcagaagggc gtggggatgc tagtggtgcg
     9841 gtattacgat ccggcgaatt aggtttgtgg ttgctgggtt gcacaaaacc gatcaccaca
     9901 gagacacaga gttcacggag ttgcaatgat catctctgtg ctctcagtgc ctctgtggtg
     9961 atcggttttt ttgcaacttt tccacccacc gtagattctc ttgaccctct tgatagcctc
    10021 ggaggcgatg acgacctctg accccagccg cgaagcgatt aagggccgcg gcgcgagctg
    10081 gaatccgcag aaccggttcg agaagctcga gtacgtgctc gatgaagacg cgtcgccgag
    10141 cgacgacgag ccggcgccgc tgccgcgaac gctctttctt cgcgatccga cccgcacgat
    10201 cctcgcgcac aacgacagtc ccgacgtcgg tttcgataca agcatcaatc cgtaccgcgg
    10261 ttgcgagcac ggctgcatct actgctacgc ccgccccagc cacgaattcc ttggcttctc
    10321 ggccgggctc gagttcgaga cgaagatcct ggtgaaggaa gacgcgcctg agctgctgcg
    10381 cgaggagctg atgtcgcgca agtacgagcc gcggatgatt gccatcagcg gtgtgaccga
    10441 tccctatcag ccgatcgagc gcaagctgga gatcacgcgg cgttgtctgg ctgtgctggc
    10501 tgagttccgc aatccggtcg gcatcatcac gaagaaccat ctcgttacgc gcgacatcga
    10561 catcctgcag gagctggccg cgcacaacgc cgtatcggtc cacatctcgg tgacgacgct
    10621 cgatccgcat ctcgccaatc tgatggagcc gcgcgcgtcg cgtccggagc tacgcattgc
    10681 ggcgatcgaa gcgctggcaa aggccgggat tcccgcgggt gtcatggtgg cgccggtcgt
    10741 gcccgcgatc acggatcatg agatgcctgc gatcctgacc gcggccgcta acgccggcgg
    10801 aacgtcggcc ggattcgtcg tcctgcgttt gccatgggct gtggcgccgc tgttcgagaa
    10861 atggctggaa gagcactatc ccgaccgcaa ggaaaaggtg atgaaccgcg tccgcgatct
    10921 gcgcgaaggg aaattctacg atccgcagtg gggcgttcgc cagcgaggca aagggatctt
    10981 cgccgatcag atcgcgtcga tgttcgaagt gacgtgccgc cgcctgcatc tcaacgaaaa
    11041 gaaatacgac ctgtccacgg acgcttttcg acggcccacg acgcaaggta gtctcttctg
    11101 accatgagcg acgagccgaa aaccgttttc gaaggaaagc acgttctggt gctggaacgg
    11161 gatacatggc agtacgtcga gcggaagaag ggaaagacag cggtgatcat cctcgccgtc
    11221 accgacgacg accgcgtgct c
//
LOCUS       DATLVW010000380         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_468729, whole genome shotgun sequence.
ACCESSION   DATLVW010000380 DATLVW010000000
VERSION     DATLVW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468729"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1954
                     /locus_tag="VNN25_11435"
     CDS             236..1954
                     /locus_tag="VNN25_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH92184.1"
                     /translation="MPERPVILIADDDEDVQRLLTLYVRPLDCDVLTARDGEAALAIA
                     QSRLPDLVLLDVMMPKRSGWEVCQALKAVQRTSHIAVVLVTGRGDVKDRLTGLQLGAD
                     DYLVKPFNRDEVVKRIAALLQRKHRDTHSAGELAKASESMLFDKASGLPTMPMVLARL
                     KEMLIEQSEIGIVYVDIEQFESIEAEYGWAFFDEFLRCAGEAVAAEARNRFPRAIVAA
                     NLVGGSSFYIFFESRGTDLRQETAFDLMANDVREKLITAMRERFPNMQQGQIGFFVGA
                     ARIDYRPQIRLERQVYQGMQSAGDTVRNAEQQRKKQLTRELRDIIRRKKVTTLFQPIV
                     WAQKSTVFGYEVLTRGLPNSSFRNSDVLFGFAREAKLAWALETVALEAALKRLRSFEL
                     DGKKFLLNLEAEMFEESEFRIHEMVSFFSEHRGNFVFELTERAAIEDYAVFRRLLDEF
                     REKGIEVAIDDAGSGYASLEAIAALAPDYLKITKGLVSTLADEPIKQDLVKMLVDLAG
                     KIGAKTLAEGLETVEEYEWCRDLGVDLLQGYYLAHPQDEPSIGDEEVEASLKADAARR
                     LLSAVR"
     gene            2094..3173
                     /locus_tag="VNN25_11440"
     CDS             2094..3173
                     /locus_tag="VNN25_11440"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH92185.1"
                     /translation="MDSFPLRPREVFEELSKTVVGQDEAIRELSVAVVKHLVGHPAPN
                     ILLVGNSGSGKTTLMRAVEQFLAGHADLIESANVIRINANVLAEEHQGYGKAVLGRLL
                     ANARRILGATASMSDLLRCVEHGIVFIDEVDKIRAQVSGQPNVRGIVAQEALLTLMEN
                     ENVEFDVDGMRFAVNSSNILFIVGGAFEELYEAVFRRATIGQDVAPMKPIVVVSASGE
                     VREELPFHLRDYLKYDDLFRYGMTPQFVSRFESIVVLNDLAESDLARIFVEPEASIFR
                     TSRAYFQRFGVDLQITRGALIAIAWDAAQQKRLGARALRETFRRVIGNLEFDPADAGT
                     RVLTIDENMVKKSIASREQGTARRQ"
     gene            3170..>4142
                     /locus_tag="VNN25_11445"
     CDS             3170..>4142
                     /locus_tag="VNN25_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92186.1"
                     /translation="MTRTSFAIVAVVGCLFAASASATVIFVDSVAVAPGQGTRQWPYA
                     TLASAASVSRVGDVIYVADSGTPYTDSIVLKKGQILVGSAFGLDALRTEFHVETGVDL
                     PAREAASPVIHGTISTSGDNIIAGCTIVAERMAGISGAGAVGTLTVRDVYFKTSLGGF
                     ALFLQDQHGKVTITGGSVDATQQGGGLGFAGGEGEISVDHCSVSGDFSAALRVTDRRL
                     GAITFRRGTKFRVADAGDDAIVLRDVDRSAPIVFEDVVTVHGHRRGFVASNVGSVKLG
                     GSSTLTTTNGAALDVRDSGAELSFESVSSSGVTPGTLDEGIVVDRVHG"
BASE COUNT          761 a         1270 c         1338 g          773 t
ORIGIN      
        1 cagattcgac ttcttcaggt agttggtgat gccctggctg gcgatgcgga cgttgctgaa
       61 agtgaaatcc tcgccgatga tgccacgcca tcccgaggtc ccgaatttga tcatggttct
      121 ccaatcaatg cggcgtggga acttaacaca ttgcctcgga gcgcggaacg aatgcgggcg
      181 agatgcacgc gctccccagg cactggcccg gttcaagcaa taagatgcgc aagctatgcc
      241 cgagcggccc gtgattctga ttgccgatga tgatgaagac gtgcagcgcc tgctgacgct
      301 gtatgtgcgc ccgctcgact gcgatgtcct gactgcccgc gacggcgaag cggcactcgc
      361 catcgcacag tcgcgcctgc cggatctcgt cctgctcgac gtgatgatgc cgaagcgcag
      421 cggctgggaa gtctgccagg cgctcaaggc ggtgcagcga acgtcgcaca tcgccgtcgt
      481 gctcgttacg ggacgaggag atgtgaagga ccggctgacc ggcttgcaac ttggagcgga
      541 cgactacctc gtcaagccgt tcaatcgcga cgaggtcgtc aagagaatcg cagccttgct
      601 ccagcgcaag catcgcgaca cgcactccgc cggcgaactg gccaaggcgt ccgagagcat
      661 gctcttcgac aaggccagcg gcctgccgac gatgccgatg gtcctggcgc ggctgaagga
      721 gatgctcatc gagcaaagcg agatcggcat cgtctacgtc gacatcgagc agttcgagtc
      781 gatcgaggcg gagtacggct gggccttctt cgacgagttc ctccgctgcg cgggcgaagc
      841 tgtggcagcc gaggcgcgga atcggtttcc aagggcaatc gtggccgcga acctcgtcgg
      901 cggatcgagc ttctacattt tcttcgaatc gcgaggcacc gatctgcgcc aggagacggc
      961 cttcgacctc atggccaatg atgtgcgcga gaagctgatc acggcgatgc gcgagcgctt
     1021 cccgaacatg cagcagggac agatcggctt cttcgtcggc gcggcccgta tcgattaccg
     1081 gccgcagatc cgcctggagc ggcaggtcta tcagggcatg cagagcgccg gcgataccgt
     1141 gcgcaacgcc gagcagcagc gcaagaaaca actgacgcgc gagctacgcg acatcatccg
     1201 ccgcaagaag gtgacgacgc tgttccagcc gatcgtctgg gcgcagaagt cgacggtctt
     1261 cggctacgag gtgctgacgc gcggcctgcc caattcgtcg ttccgcaatt ccgacgtcct
     1321 cttcggcttc gcccgcgagg ccaagctggc ctgggcgctg gagacggtag cgctcgaagc
     1381 ggcgttgaag cgcctgcgct ccttcgagct cgacggcaag aagttcctgc tcaatctcga
     1441 ggcggagatg ttcgaggagt cggagttccg catccacgag atggtctcct tcttctcgga
     1501 gcaccgaggc aacttcgtgt tcgagctgac ggagcgcgcc gcgatcgagg actacgccgt
     1561 cttccgaagg ctgctcgacg agttccgcga gaaggggatc gaagtggcca tcgacgatgc
     1621 cggctccggt tacgcctcgc tcgaagcgat cgccgcactg gcccccgact acctgaagat
     1681 cacgaaaggc ctggtgtcga cgctggccga cgagccgatc aagcaggatc tcgtgaagat
     1741 gctggtcgac ctggcgggca agatcggcgc gaagacgctg gccgagggcc tggagaccgt
     1801 cgaggaatac gaatggtgcc gcgacctcgg cgtcgactta ctccaaggct attacctcgc
     1861 ccatcctcag gacgagccat cgattggcga cgaagaggtg gaggcgtcgc tgaaagccga
     1921 cgccgcgcgc aggttgttga gcgctgtgcg ctaagccgtg tttcagctct ggcctctttt
     1981 caacccttga acgtttgcgc tcgattgaaa ccgaagtacg gtaggtgtga gaaatgcggg
     2041 cgtggacgga gtgctattct tccgcgactc caatcgtctt ccaatcacat cctatggact
     2101 cctttccact ccgcccacgt gaggtcttcg aagagctgag caagaccgtc gtcggtcagg
     2161 atgaggcgat ccgcgagctc tccgtcgccg tcgtcaagca cctggtcggg catcccgcgc
     2221 cgaacatcct gctcgtcggc aactccggtt ccggaaagac cacgctcatg cgcgcggtcg
     2281 agcagtttct ggccggacac gccgatctga tcgaatccgc gaacgtcatt cgcatcaacg
     2341 cgaatgtgct cgccgaggag caccagggat atgggaaggc ggtgctgggc cggctgctcg
     2401 ctaacgcgcg gcgaattctt ggcgccaccg cttcgatgag cgatctcctt cgctgcgtcg
     2461 agcatggcat cgtcttcatc gatgaagtcg acaagattcg cgcgcaggtc agcgggcaac
     2521 cgaacgtgcg cggcatcgtg gcgcaggaag ctctcctgac gctgatggaa aacgagaacg
     2581 tcgagttcga cgtcgacggc atgcgcttcg ccgtcaactc gtcgaacatt ctcttcatcg
     2641 tcggcggcgc cttcgaggag ctctacgagg ctgttttccg tcgcgccacg atcgggcagg
     2701 acgtggcgcc gatgaagccg atcgtcgtcg tttccgcctc cggcgaggtt cgtgaagagt
     2761 tgccgtttca tctgcgggac tacctgaagt acgacgatct gttccgttac ggcatgaccc
     2821 cgcagttcgt ctcccgcttt gaatcgatcg tcgtgttgaa tgatctcgcc gagtccgatc
     2881 tggcccgcat cttcgtggag ccggaggcct cgatcttccg gacgtcgcgg gcatactttc
     2941 agcggttcgg cgtggacctg cagatcacgc ggggagcgtt gatcgccatc gcctgggacg
     3001 cagcccagca gaagcgtctc ggagcccgcg cgcttcgcga gacgttccgg cgtgtgatcg
     3061 gcaacctcga gttcgatccg gcggacgccg gcacgcgcgt gctcacgatc gacgagaaca
     3121 tggtcaagaa gtcgatcgcc agccgcgagc agggaacggc ccgccgccaa tgacgcgaac
     3181 gtcattcgca atcgttgcgg tagtcggctg cctcttcgcc gcgagcgcat cggcgactgt
     3241 gatcttcgtc gacagtgtgg ccgtcgcgcc gggacaaggc acgcggcagt ggccttatgc
     3301 gacgctggca agcgccgcca gcgtcagcag agtgggcgat gtgatctacg tcgccgattc
     3361 gggcacgccg tacaccgaca gcatcgtatt gaagaagggt cagatccttg tcgggtcagc
     3421 gttcggcctc gatgctctgc gcacggagtt tcatgtcgag accggcgtcg accttcctgc
     3481 cagggaggca gcgtcccccg tgatccacgg cacgatttcg accagcggcg acaacatcat
     3541 cgccggatgc acgatcgtcg cagagcggat ggcgggaatc agcggggcag gcgcagtcgg
     3601 gacattgacg gtgcgcgacg tttacttcaa gacgtcgctg ggcggatttg ccctctttct
     3661 tcaggatcag catggcaagg tgacgatcac cggtgggtcg gtcgacgcaa cgcaacaggg
     3721 cggcggcctt ggcttcgccg gcggcgaagg ggagatcagc gtcgatcact gctcggtttc
     3781 cggcgatttc tcggccgcgc tgcgagtcac ggatagacgc ctcggcgcca tcacattccg
     3841 gcgcggcacg aagtttcgtg tcgccgacgc aggcgatgat gcgatcgtct tgcgcgatgt
     3901 cgaccgaagc gctccgattg tgttcgagga tgtcgtcacg gttcatggcc accgccgcgg
     3961 attcgtcgcc agcaacgtcg gcagcgtgaa gctgggcggg agctcgaccc tcaccacgac
     4021 gaatggcgct gcactcgacg tccgcgattc gggcgcggag ctttcgttcg aatccgtctc
     4081 ctcctcgggc gtgacgccgg gaacgctcga cgagggaatc gttgtcgatc gcgtacacgg
     4141 tc
//
LOCUS       DATLVW010000381         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_386660, whole genome shotgun sequence.
ACCESSION   DATLVW010000381 DATLVW010000000
VERSION     DATLVW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386660"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VNN25_11450"
     CDS             <1..1139
                     /locus_tag="VNN25_11450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH92187.1"
                     /translation="VDEVVQEAARLAVAGTNVNAVKANDALCSELAARIASTFDQQSV
                     RFVIVENGTLPDNPLFTEALYRAISDYGNRHGFGKYVLWHDHDLMWSAEPHRFGAYPY
                     SGVRKPKANQHVEYSVSTDWMRKRMQAWSPGLPCHIIPDRFFVPPPETQPRKALRAQY
                     GIPKDAYLIARCTRVVPQKSIERDLRLLGQLQRRLSKPVFLFITGPTTEDPAEFEYLR
                     ALARKLFIADQVIWGDGLLPFNRLLGDPAGNAERLHVFDLLAEADLSSFLTTYDYEGF
                     GNPPGEAMAARVPFIATTYELYQDVYGSKGVIAPLLAIDRATSPADPMPEDFLAWTVR
                     ALTDDAYRKQTTAHNLEICQRFFSLDALERQVVEIFDIKVLESQ"
     gene            complement(1117..2634)
                     /locus_tag="VNN25_11455"
     CDS             complement(1117..2634)
                     /locus_tag="VNN25_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXH92188.1"
                     /translation="MGTVILTEDAGFVAAVAFSADGEWLAAGGSSGIVFLFSLADMTN
                     PRRLVGHAGRIRSLAFSAQSDMLASGAEDRDVILWSSADGARLATLSGHSGDVMALSF
                     SPSGHCLASASHDHTVRLWSTVRKECLGQLYSTAARVWAVQCSPNGRTLSTASEDSAI
                     RVWDIESRRRLFTLRGYSNKTWSLAFAPQAERLVAANEDGVARVWNTRDANTVLELRG
                     HASRVWAIACASNGKWICSASDDLTVRLWDLESGRCRQTMRGHTDWIRSVAFDVTSQI
                     IASAAEDGRIRLWDVDTGICLASIDSQMPRLLTIAFCDDGRMIAAGGSAQSVHLFSVD
                     DGSFLGVLPGHTGWLTAVVPLGTSMLASCSEDATIRIWDVAKRELLQLLEVGSRVRCG
                     AFCDDGLSFLSGSDDGLLRRWNVATGRCEDEIRAHHSSVWSVAVNAAQDNVATAGDDG
                     AIRLWRLPRFETSHFPDVLRPPRPYEAMNITGATGLSPEQRNALMMLGAIEIPAP"
BASE COUNT          619 a          883 c          803 g          561 t
ORIGIN      
        1 acgtcgacga ggtcgtgcag gaagctgcac ggctcgcagt agcgggcacc aacgtcaacg
       61 ccgtgaaagc gaatgatgct ctctgtagcg agcttgctgc tcggattgcg tccacgttcg
      121 accagcagag tgtgcgattc gttatcgtcg agaacggcac cctacctgac aacccattgt
      181 tcacagaggc tctctatcgc gcgatctccg attatggaaa tcgtcacggt tttggtaagt
      241 acgttctatg gcacgaccac gatctcatgt ggtcggcaga accacatcgt ttcggcgcct
      301 atccgtactc cggggttcga aagccgaagg caaaccagca tgtcgaatat tcggtgagca
      361 cagattggat gcgaaaaagg atgcaggcgt ggtcgcccgg attgccctgc cacatcatcc
      421 cggatcgttt cttcgtgcct ccgccagaga cgcagccaag aaaagcactt cgcgcccaat
      481 atggaattcc caaggatgcg tacctcatcg cgcgctgtac gcgagtagtt ccccaaaaat
      541 ccatcgagcg ggatttgcgc ctcctcggcc aactgcagcg acgcctctcc aaacccgtgt
      601 ttctcttcat aaccggcccg acgaccgagg atcctgctga attcgagtat ctccgcgcct
      661 tggcgcggaa gttattcatt gccgatcagg tcatctgggg cgacggtttg ctgccattca
      721 acaggcttct cggcgatccc gccggaaatg cagagagact tcatgtcttc gacctgctcg
      781 ctgaagcgga tctgagttcg tttctgacga catatgacta cgaaggcttt ggtaaccctc
      841 caggcgaagc aatggccgcg cgcgttccgt tcatcgccac gacatacgag ctctatcaag
      901 acgtgtatgg gagcaagggt gtcatcgcac cactgcttgc catcgaccgt gcgacctcgc
      961 cggccgatcc gatgcccgag gactttctcg cctggacggt acgagcgcta acggacgacg
     1021 cgtatcgaaa gcagaccacg gcccacaact tggaaatttg tcagcggttc ttttcccttg
     1081 atgcgcttga gcgccaagtt gtggagatct tcgacatcaa ggtgctggaa tctcaatagc
     1141 acccagcatc atcaacgcgt tccgctgctc ggggctcagt cccgtcgcgc cggtgatgtt
     1201 catcgcctcg tagggacgag gcgggcgcag cacgtcaggg aagtgagagg tctcaaatcg
     1261 cggcagccgc caaagacgta tggcgccgtc gtcgccggcc gtagcgacat tgtcctgcgc
     1321 agcgttgacg gccacactcc agacggacga atgatgggcg cggatctcat cttcacacct
     1381 gccagttgca acgttccagc ggcgtagcaa accgtcatct gagccgctga gaaacgagag
     1441 tccgtcatcg cagaatgccc cacatcgcac cctcgaacca acctcgagca gctgcaacag
     1501 ttcgcgcttc gcgacatccc agatccgaat cgtcgcgtct tcgctgcaac tggcgagcat
     1561 ggacgtgccg agcggaacga cggcagtgag ccatccggtg tggccgggaa gcacaccaag
     1621 gaacgagccg tcgtcaacgg agaacagatg gacgctctga gctgatccgc ctgcggcaat
     1681 catccttccg tcgtcgcaga aggcaatcgt caggagccgc ggcatttgac tgtcgatcga
     1741 tgcaagacaa atcccagtgt cgacatccca cagacgaatc cggccgtcct ccgcagcact
     1801 ggcgatgatc tgcgacgtga catcgaacgc aaccgaacgg atccagtcgg tatgaccgcg
     1861 catcgtttgc cggcatcgcc ccgactccaa gtcccacaat cgcacggtaa ggtcgtcact
     1921 agcgctgcag atccatttcc cattcgaagc gcacgcgatg gcccagactc ggctggcgtg
     1981 gccgcgcaat tccaggactg tgttggcatc gcgcgtattc cagacgcgtg cgacgccgtc
     2041 ctcattcgca gcaacgagac gctcggcctg gggcgcgaag gccagcgacc atgtcttgtt
     2101 tgaatagccg cgtagtgtaa aaaggcgcct gcgagattcg atatcccaca cgcggatggc
     2161 gctgtcctca ctggcggtcg agagcgtacg accatttgga ctgcattgga ctgcccacac
     2221 ccgcgccgct gtcgaatata actggccaag gcattccttg cgaaccgtgc tccaaagacg
     2281 tacggtatgg tcgtgacttg cgcttgcaag acagtgtccg ctggggctga aggaaagcgc
     2341 catgacgtcg ccggaatggc cggaaagcgt tgccaatcgc gcgccgtccg ccgacgacca
     2401 caggatcacg tcgcgatcct ctgcgccgct agcgagcatg tcactctgcg ccgaaaaagc
     2461 aagcgagcga attcgcccgg catgtccaac aaggcgccgc ggattggtca tgtcagctag
     2521 agagaaaaga aagacgatgc cgctgcttcc accggccgca agccattctc catccgcact
     2581 gaatgctacg gccgcgacga agcctgcgtc ttctgtaagg atgacggtgc ccatacccgt
     2641 ctcgagatcc cagatgcggc acgttccgtc ttcactgacg ctagcgaggg ttttgccatc
     2701 cgcactgtac gcgatcgaat aaatccagct ttcatggcct atcaggacat tcgtacagcg
     2761 accgtccttc gtgctccaga aacggatcgt atgatcgaag ctgccggacg cgatcgtgtt
     2821 tccatctggg ctgaattcga cagcgacgac aatgtcagcg tgtcca
//
LOCUS       DATLVW010000382        10308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_386666, whole genome shotgun sequence.
ACCESSION   DATLVW010000382 DATLVW010000000
VERSION     DATLVW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386666"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VNN25_11460"
     CDS             complement(<1..692)
                     /locus_tag="VNN25_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HXH92189.1"
                     /translation="MPPEPKFNFGVIEGFFGKPWDWSARLSGADFLSDWGYRFYIYAP
                     KADPFLRRRWREQMPVQAMQHLSELNSRCQHRGISLGIGLTPFEIYLDYDSGAKAALR
                     SKVLQLNEVQADMLCILFDDMRGDVTDLPDIQAKVVSDVCGWSNAKGFIVCPTYYSYD
                     SRLIREFGPPPKTYLRDFGRLIDPTVDIFWTGEKVISDGYTAQHLEEVADDILRKPFI
                     WDNHISNDSRIRT"
     gene            916..1398
                     /locus_tag="VNN25_11465"
     CDS             916..1398
                     /locus_tag="VNN25_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXH92190.1"
                     /translation="MDLLINGKTFQLSAGGDTPLLWVLRDHLGMTGTKFGCGMALCGA
                     CTVHVDGKAIRACVVSVSSLAGKKITTIEGIGETAPGEALQQAWVALNVPQCGYCQAG
                     QIMSAAALLASSSQPTDLDIDAAMAGNICRCGTYPRIRAAIKRAALRMPKLDLVEGAK
                     "
     gene            1395..3674
                     /locus_tag="VNN25_11470"
     CDS             1395..3674
                     /locus_tag="VNN25_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXH92191.1"
                     /translation="MTMIASPSRREFLVNLKALGGLVLVASVSDLVKAEEKYGADGMP
                     HGWSDNPLIFVAIGGDGTVTIVVHRSEMGQGVRTGMPLIVADELEADWSRVRVTQAPA
                     DEEKYGNQDTDGSRSTRHFFEPMRRCGAAARTMLEQAAAQVWNVPVAQVHAANHAVIH
                     AASGRKLDYGALAQRAAKLPVPARDSLRLKSPAQFLYIGKGETPLADGWDIATGKAQY
                     GIDSVADGMLFAVIARSPTLGGKIASVDSTEAEKIPGVVKVMRLQANAQKATFQPLAG
                     VGVIASDTWVAIKGRNALKIQWDEGPNGSYDSSSYRAELEHAARQPGKVLRSSGDFDG
                     ALSKASRKIQAEYYLPHIAHATMEPPAALARIVDGRCEVWAPTQAPQVTREDVALHLG
                     ISPENVTVHVTLLGGGFGRKSKPDFAIEAALLSREMGGKPVKVVWTREDDLHHDFFHT
                     VSVEHLEAGLDAQGKVVAWMHRSVAPSIAATFGPDPKHEAPFEVGMGLINLPYKIDNL
                     RIENPEATSHTRVGWFRSVSNIPHAFAVQSFIAELAAAANRDHRDFLLELLGPQRLID
                     PTAMGDSWNHGESPKRYPVDTARLRRVIEVATREARWGRKMPKGSGLGLAAHYSFVSY
                     TAAVVEVTVNEKGEIEIPSVDVAFDCGPQVNPERVRSQLEGAVIMGLSLALTGEITFR
                     RGHVQQDNFNSYRVMRMNEAPRDIRVHLLPTSDWSIPLGGAGEPGVPPIAPALCNAIF
                     AASGKRIRQIPIKDQLRRV"
     gene            complement(4553..5326)
                     /locus_tag="VNN25_11475"
     CDS             complement(4553..5326)
                     /locus_tag="VNN25_11475"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXH92192.1"
                     /translation="MAGNGSLFDLSGRVAVVTGGNRGIGRAMALGLARAGASVAVLAR
                     DAEHNKLVLAELKTIGKSALVLPLDVTDRAALATAVAEVEGTLGGIDILVNNAGIVAL
                     TGGVLNETADIWDKTIETHLNASFLLSQLVARSMVRRKSGGKIINLASMYSIFGSGLV
                     PSYSAAKGAIVQLTKSLAIELAPHNIQVNAIAPGWIETDMTAAVRSSPMNDEIIARTP
                     AKRWGKADEIIGATVFLSSRGADFITGATLPVDGGYSIY"
     gene            complement(5626..6420)
                     /locus_tag="VNN25_11480"
     CDS             complement(5626..6420)
                     /locus_tag="VNN25_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH92193.1"
                     /translation="MLDVIFARSIGVRIYGLGAVVLGLTGLFWGDFAVVWQPRNSSPG
                     QSPWGYAVAVLPLLAGLAMQWRRATWLSALTLTVLYCLAVIFLDVPGGIAQPSVFVAW
                     FGVAESLALAAGALLIYAYTAQLAPATAERLSKIGRLVFGICLIYFGLAHHFFLAFTV
                     KMVPAWLPPGQLFWAYATAVGHIAAGVAILSGVYARAASMLLTAMFVVFAILVHAPLI
                     LIDPHTHMHWAENAVNIALIGSAWVIAASINAGGKGSSTHGGLGIG"
     gene            6636..7598
                     /locus_tag="VNN25_11485"
     CDS             6636..7598
                     /locus_tag="VNN25_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXH92194.1"
                     /translation="MASSTFSRAARTNLDLDTLRTLAVARDRGGLAQAAEHLGRTPSA
                     ISLQMKRLQEDLGTPIFRKRGRGLALTETGEIALSYARRILGLNDELLDKMQGANLAG
                     HIRVGCPQDFASILPSVLSLFASLYPRMQIELHIDGNAALRDAIDNARLDVAVIIGHE
                     DRAGAQIVGRLELAWIASSIFSIPQKQPLPLAVLGSQCIFRRTALQRLEAANIPHRIS
                     ANSHSLNGLWAALLGGLGVTARTPLNLPEGLVSAESLHGLPPLGSLPVTLHRYAGSSG
                     VTSDRMASLLVEALELVLQPKSTTKGVARVRDDVVTRVAKHKTR"
     gene            7693..8175
                     /locus_tag="VNN25_11490"
     CDS             7693..8175
                     /locus_tag="VNN25_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXH92195.1"
                     /translation="MIKRATLLAALLTVSASAMAQDASIVGTWVLTAAEKLLPDGKRV
                     PDYGPNPHGLAIFTADGYYSVQIYRAERLKFSSGDKFKATLEEYKDASLSTSVHFGRY
                     TVDPVKHTITFQIDRSSVPNLDDTTAVRQYEMKGDELSWRVAPRQDGSIPVTTLRRVK
                     "
     gene            complement(8530..9279)
                     /locus_tag="VNN25_11495"
     CDS             complement(8530..9279)
                     /locus_tag="VNN25_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXH92196.1"
                     /translation="MIHFNTPPVHNGICTKRTNSAELRVLAAALVAAILGLHSGLSQA
                     TESAGPPAMAPMAAPPAGAYKLDKSHASLVLRASHMGFSTYTTRFSRFDVDLTFDPRN
                     LPASKVVTTIDASSFEMDAAPQMCLDIMKGPQMLDTTKFPQIVFKSEGIRMTGAQSME
                     ISGTLTLHGVTRPTVLVATYNGGYAGMPNMDPQARVGFSAHGSFKRSDFGIAYGVPAP
                     GTTMGVGDLIDFSIEAEFTGPPLATPGAQTH"
     gene            9574..9951
                     /locus_tag="VNN25_11500"
     CDS             9574..9951
                     /locus_tag="VNN25_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXH92197.1"
                     /translation="MKVMVFVKATKDSEAGVPPDPKMMEAMGKYNEALIAAGILKEQI
                     LGGLKPTRFAKRVHFSGKHRTVIDGPFVETKELVAGFWLWEVQSVDEAVEWVKKCPNP
                     MLTDSDVEIRPLYSLEDITRWKT"
BASE COUNT         2206 a         3041 c         3014 g         2047 t
ORIGIN      
        1 gttcgaatcc tcgagtcgtt cgagatatgg ttatcccaga tgaatggctt gcgcaggatg
       61 tcgtcggcaa cttcctctag gtgttgcgcc gtgtatccat cggaaatgac cttctcgccc
      121 gtccagaaaa tgtcgacagt cgggtcaatg agtcgtccga aatcgcgaag gtaggtcttg
      181 ggcggaggtc cgaattcgcg tatgagacgc gaatcgtacg aatagtaggt cgggcagacg
      241 atgaatccct ttgcgttact ccacccgcag acgtccgaga ctaccttcgc ttgaatgtca
      301 ggcaggtcgg tgacgtcacc gcgcatatcg tcgaacaaga tacatagcat atctgcctgg
      361 acttcattga gctgtagaac cttcgaacgc aaggccgctt tagcccctga gtcgtagtcc
      421 aagtagatct caaagggagt taggccgatc ccgagagaaa taccccggtg ttggcaccgc
      481 gaattgagtt ccgatagatg ctgcatcgcc tgaaccggca tctgctcgcg ccaccgacga
      541 cgtagaaacg gatcagcctt cggcgcatag atgtagaaac ggtatcccca gtcgctcaga
      601 aaatcagccc cgctcaaccg cgcagaccag tcccaaggct ttccgaagaa gccctcgatt
      661 accccgaagt taaacttggg ctcaggtggc atagggtcgc gcttgcagtg ggtctggaac
      721 gaccggtttg tggcggtaat cttagacgga tacgcacgga atcaagcctt gctttgctgt
      781 gccattgaaa gatagcgtat ctgtgctttc aagggcttat ctcctgatta gtcggatgtt
      841 tgtggcacac ctgtgtgatc gtaagtgatg gtttttattg actaatttga cactccgtca
      901 gcagaaggac gcctcatgga cttattgatc aatggcaaga cttttcagct atccgccggc
      961 ggcgatacgc ctctgctgtg ggtcctacgc gaccaccttg gaatgaccgg cacgaagttc
     1021 ggttgcggca tggcgctgtg cggcgcctgt accgtccatg tggacggcaa agcgattcgc
     1081 gcctgtgtcg tgtcagtctc gtcgctggcc ggcaagaaaa tcaccaccat cgagggtatc
     1141 ggcgagacgg cgcccggcga ggccttgcag caggcctggg ttgcgttgaa cgtgcctcaa
     1201 tgcggctact gccaagcagg acaaataatg tccgccgccg cactcctcgc gagcagctcg
     1261 cagccgacgg acctcgatat cgacgccgcc atggctggca acatttgccg ttgcggaact
     1321 tatccgagaa ttcgcgcagc gataaaacgc gccgcgctgc ggatgccaaa gctcgatctg
     1381 gtggagggcg ccaaatgacc atgatcgcaa gcccatcgcg tcgggaattt ctcgtgaact
     1441 tgaaagcgct tgggggcctc gtgcttgttg ccagcgtttc ggacctggtc aaggccgaag
     1501 agaaatatgg tgccgacggc atgccccacg gctggagcga caatcccttg atatttgtcg
     1561 cgatcggcgg cgacggaacg gtcacgatcg tcgtgcatcg atccgaaatg gggcaggggg
     1621 tgcgcaccgg catgccgctg atcgttgccg atgaactcga ggcggattgg agtcgagtgc
     1681 gcgtcactca agctcccgcc gatgaggaga agtacggcaa tcaggatacg gatggatcca
     1741 ggagcacacg ccattttttc gagcccatgc gccgctgcgg cgcggcggca agaacaatgc
     1801 tcgaacaggc ggccgcgcag gtctggaacg tccctgtcgc tcaagtgcac gccgccaacc
     1861 acgcagtcat ccacgcggca agcggacgaa agctggatta cggcgcgctg gcgcaacgcg
     1921 ccgcaaaact gccggtgccg gcgcgcgatt cccttcgcct caagtcgccg gcacaatttc
     1981 tctacatcgg caagggggaa accccactgg ccgatggatg ggacatcgcg accggaaaag
     2041 ctcagtacgg aatcgactcc gtcgccgacg gaatgctgtt tgcagtcatt gcgcgctcgc
     2101 ccaccctggg agggaagatc gccagtgtgg attcaacgga ggctgagaag attcccggcg
     2161 tcgtcaaggt catgcggctt caggccaacg cacagaaggc gacattccag cctctcgccg
     2221 gcgtgggggt gatcgcgagc gatacctggg tcgcgatcaa gggacgcaat gcgctcaaga
     2281 tccagtggga tgagggaccc aacggctcgt atgattccag ctcgtaccgg gccgagctgg
     2341 aacatgccgc gcgacagccg gggaaggtct tgcgcagctc gggagatttc gatggtgcgc
     2401 tctccaaggc atcccggaaa attcaggcgg aatactacct gccgcatatt gcgcatgcca
     2461 cgatggagcc gccggcggcg ctcgctcgta tcgtcgacgg ccgttgtgaa gtctgggccc
     2521 cgacacaggc gccgcaagta acgcgcgagg acgttgcatt gcatctgggc atttcccccg
     2581 agaacgtcac ggttcacgtg actctgctcg gcggcggctt cggacgaaag tccaaacccg
     2641 actttgccat cgaggcggcg cttctgtccc gcgagatggg cggcaagccg gtcaaggtcg
     2701 tctggactcg cgaagacgat ctccaccacg acttctttca tacggtttcg gtcgagcatc
     2761 tggaagcggg tttggatgca cagggcaaag tggtcgcctg gatgcatcgg agcgttgcac
     2821 ccagtatcgc cgcgacattc ggccccgatc cgaagcacga agcaccgttc gaggtgggca
     2881 tgggcctcat caacttgccc tacaagatcg ataacctgcg catcgagaat ccggaagcga
     2941 cgtcgcatac gcgcgtcggc tggtttagat cggtctccaa tattccgcac gcgtttgcgg
     3001 tgcagtcctt catagcggag ctcgcggctg cggcgaatcg cgatcaccgc gattttctcc
     3061 tggagctatt gggtccgcag cggctcatcg atccgaccgc gatgggcgac tcctggaatc
     3121 acggcgagtc gccgaaacgt taccccgtcg atacggcgcg tctacgccga gtgatcgagg
     3181 tcgccacccg tgaagctcgc tggggccgaa agatgcccaa gggaagcgga ctcgggctcg
     3241 ccgcacacta tagcttcgtg agctacacgg cagccgtcgt cgaagtcacg gtcaacgaga
     3301 agggagagat cgagatcccc tccgtcgatg tggcctttga ctgcggcccg caagtcaacc
     3361 cggagcgcgt gcgctcccag ctggagggag cggtgatcat ggggttgagc cttgccctca
     3421 cgggcgagat cacttttcga cgagggcacg tgcagcagga caacttcaac tcgtaccgcg
     3481 tcatgcgcat gaacgaagcg ccgcgcgata tccgcgtgca tttgttgccc acgagcgatt
     3541 ggtccatacc tcttggaggt gcgggcgagc ccggtgtgcc gcccatcgcg ccggcactgt
     3601 gcaatgccat cttcgcggcc agcggcaagc gcatccggca gattccaatc aaagatcaac
     3661 tgcgccgggt ctagtgcacc ggccagttgt cacgttgaag tgaaggtgcg ggtctcaaca
     3721 cgacccgagt gggtccaacg cctcggcttc acggggaacc actgcctcgc catcgacaaa
     3781 cgtattgtcg ggcacaccgt caactgcctc ggttacgacc tcgtgtcgac ccattccggc
     3841 ggatggtcag cggcagcttc agggcagcca gtagggggcc gtggactcat tcgatcagga
     3901 gtcctggcag gggcacgcac tggcgcagtt agggcttaag ggccgcgatt aaggtgctct
     3961 cgtcacgaac gcattcccag ttgatcaatt taaaccggcg ctcgaaggcc cgagcctcta
     4021 cttggcagtc acctttgctg ccgtacccgt gcaggacgtc gcggtgtacg gctccgtgtg
     4081 cggtgtagat caggaagacc agagtccaac tcatgattca aacctccaaa agtatttaaa
     4141 tacctctgat tgaaccggat tccagacgat ctccaagatg ttcgatactt aaattacagt
     4201 gatggcaatg ccatccgcga aacctatcgc gacacagtgt ggcagacagg tccctttcgc
     4261 agataaggac atacgagcac tgcgacgcgt attccacaaa agatcgctgt aagttaaatt
     4321 ggaaccgatc gtggttagag gcgcagagca taagcaaaag acaaatggcg tacctttcct
     4381 ttcgcgcggc ctcgcctcta accccctgcg gccggacgcg accggcagct atcgggtagg
     4441 acagttacat tgcctgggaa gacacctaat accttcggta ccacccaccg gcctgagggg
     4501 tgaacacgca ccggccgaat atgagcgtcg gcttttcggt ccttcagcga tcttagtaaa
     4561 tcgaatagcc tccgtcgacc ggcagcgtgg cgcccgtaat gaagtcggct ccgcgagacg
     4621 aaagaaaaac ggtcgctccg atgatttcgt cagcttttcc ccaacgcttt gcgggtgtgc
     4681 gggcgatgat ttcgtcgttc atcggtgagc tacgcactgc agccgtcatg tccgtttcta
     4741 tccagcctgg cgcgatggca ttgacttgga tgttgtgtgg cgccaattcg atcgcaagcg
     4801 acttggtgag ctgaacgatg gctcccttgg cggcgctgta ggagggcacg agccctgacc
     4861 caaagatcga gtacatgctc gcgagattga tgatcttacc gccgcttttg cgcctgacca
     4921 ttgatctcgc aaccagctgc gaaagtagaa aagaagcgtt cagatgggtt tctatcgtct
     4981 tatcccagat atccgcagtc tcgttcagca ccccacctgt tagcgcaacg atcccggcat
     5041 tgttgaccaa tatgtcgatg ccaccaagcg taccctccac ttcagccact gcggtagcca
     5101 atgcagcccg atccgtcaca tcgaggggca gcaccagcgc ggacttgccg atggtcttca
     5161 gctccgcgag taccagttta ttgtgttccg catcgcgcgc gagcacagcc accgatgcgc
     5221 cggcgcgcgc caatcctaat gccatggcgc ggccgattcc tcgattgcca ccggtcacca
     5281 cggcaacacg gccactcagg tcaaaaagcg aaccattgcc ggccattgtg cctcctggga
     5341 agcggcggtc agaagaaaaa ctcgagccgc gtctgttatg ggtatcgcta actgccgatt
     5401 gagactacct gagatccgga ttgtccgctt cagtgaatcc tgttcgatga tgcgaacgtc
     5461 tcaaagtggc cccgataggt tgagttggcc caggagactt gatgggaaag tagagctgag
     5521 tggaacagct gaatgaacgc ttcctgcgga cgctgcaatc ctcgggattc gcagcgccac
     5581 gaatgaacca ccgcatcacc gtcatacaat cgctggatcc aagattcagc cgatgccaag
     5641 gccaccatgt gtcgaagaac ctttaccgcc ggcgttgatc gacgccgcga tgacccacgc
     5701 agaaccgatc aaggctatgt tgaccgcgtt ctcggcccag tgcatatgag tgtgggggtc
     5761 gatgaggatc agcggcgcat gcactaggat agcgaaaacg acgaacatcg ccgtcaggag
     5821 cattgaggcc gcccgcgcgt agacaccgga aagaatggcg acgccggctg caatatgtcc
     5881 caccgcggtc gcgtacgccc agaaaagttg acctggcggc agccacgcag gaaccatctt
     5941 gaccgtgaag gcaaggaaga agtggtgagc caagccaaaa taaatgagac aaatgccaaa
     6001 gacgagacga ccgatcttgg atagtcgctc ggcggtggcg ggtgcgagtt gcgcggtata
     6061 cgcgtaaata agcaacgccc cagctgcgag cgccagactt tcggcgacgc cgaaccaagc
     6121 gacgaacacg gagggctgcg cgatgccacc aggaacgtcc agaaatatga ccgccaaaca
     6181 gtaaagcacg gtaagcgtga gagcgctgag ccatgtggcg cgtcgccatt gcatggccaa
     6241 acccgccagc aacggaagga ccgccacggc atacccccag gggctctgcc ccggagagct
     6301 gttccttggt tgccacacca ccgcgaaatc accccagaac aagccggtaa gccctaacac
     6361 aacagctccg agtccgtaga tgcggacacc gatactccgc gcaaatatta cgtccaacat
     6421 agatgccact gaaaattctc cccaaaaacg tcttaggtca tgagcctctg aatgactcct
     6481 ttgcggctcg catcttagcg aaaacgccgg cagccaattt gctgcatggc attttcgggt
     6541 tcgtacgtta ggtatcgatc gagtcggttg gttcaactcg gccattatcg gcataatcgc
     6601 ttccgcatca tacgcaggca ggagtggaca agcacatggc ttcttcgacg ttctccaggg
     6661 cagcacgaac caacctcgat cttgacacct tgcgcaccct tgctgtcgcg cgcgaccgcg
     6721 gaggcttggc gcaggcagcc gaacatctcg gccgcacgcc gtcggccatc agtctgcaaa
     6781 tgaagcgcct gcaggaggat ctcggcacgc cgatcttccg taaacgcggt cgtggtctcg
     6841 ctttgaccga aacaggggag atcgcactct cctacgcgcg tcgtattctc gggttgaacg
     6901 atgagttgct ggacaaaatg cagggcgcga acctcgccgg acacattcgc gtcggatgtc
     6961 cgcaagactt tgcgtcgatt ctgccttcgg tcttgtctct cttcgcgtcg ctctatccgc
     7021 gcatgcagat tgagcttcac atcgatggga acgctgctct tcgcgatgcg atcgacaacg
     7081 cgcgcctcga tgtcgcggtc atcatcgggc acgaagatcg tgctggcgcg caaatcgtgg
     7141 ggcggctcga gctcgcgtgg attgcctcat ccatcttcag cataccgcag aaacagccac
     7201 taccgctggc tgtgctgggt tcgcagtgca tcttccgcag gaccgcctta caacggctcg
     7261 aggccgcgaa tataccgcac cgtatttccg ccaacagcca tagcctgaat ggcctttggg
     7321 ctgcattgct cggcggtctc ggcgttactg cacgcacgcc gctcaacctt cccgaggggc
     7381 ttgtatctgc tgagtcgctg cacggcttgc ccccgcttgg tagcctgccc gtcaccttgc
     7441 accgctatgc cggctccagt ggtgtcactt cagatcgcat ggcgtctctg ctcgtggaag
     7501 ctctcgagct cgtcttgcaa ccgaagtcga ccaccaaagg cgtcgcccgc gtgcgagacg
     7561 atgtcgtgac tcgcgtcgcg aaacataaga cacgttaacc agtcgcttga gaagaatcaa
     7621 attgtgcagg gtgcggacgc ctgattacat tgcggctatc gcgccccggc gcatcactac
     7681 acggattgga tgatgatcaa gcgtgccaca cttctcgccg ctcttttgac tgtctccgct
     7741 tcagctatgg cacaagacgc ctcgatcgta ggtacgtggg tcctcaccgc agctgagaag
     7801 ttacttcctg acgggaagcg cgttcccgac tatggtccga acccgcacgg gctggcgatt
     7861 ttcaccgcgg atgggtacta ctcggtgcag atctatcgcg ccgaacggtt gaagttctcg
     7921 tcgggtgaca agttcaaggc gactctcgag gagtacaagg atgcgtcgct cagtacaagc
     7981 gtgcactttg gtcgctacac cgtcgatcca gtcaaacaca cgatcacgtt ccaaatcgat
     8041 cgttcatccg tgccgaatct agatgacacc acggcggtgc gtcagtatga gatgaagggc
     8101 gatgagctct cgtggagggt tgctccgcgg caggatggct ctatccccgt caccacatta
     8161 cgacgggtca agtagagcgg acgaaagaag cgcagcagca gaaaggaact ctcgagtttc
     8221 aagccagtgc catctggccg catgtcaccg agaccaatgc tttctgtacg tcctgccatg
     8281 agatgaaaac agaaggtcct gtttgccgga atcatcattc ccgcgatcgc gaacattcag
     8341 cgcccggaag cgcacaagcg gctcatgtgc ggacaagatg gacgctatgg aaaaagctgc
     8401 acgccggaag aatctcgcgt tcgctaagaa gttggatgcc ggattccgtc acaacgagcg
     8461 cgtatcgagc ccgagcggtc gtcggtggac gactgcttag gaggaggatt cgttgtacgg
     8521 ggaatcccct caatgcgtct gagcgcccgg cgttgccagc ggcggtccgg tgaactccgc
     8581 ttcgatcgag aaatcgatca agtcaccaac gcccatcgtc gtacccggag caggcacgcc
     8641 ataggcgatg ccaaaatctg agcgtttgaa cgacccgtgc gcggaaaatc ctacgcgcgc
     8701 ctgcggatcc atgttcggca tgccggcata gccgccgttg taggtcgcca caagcaccgt
     8761 cggccgcgtg acgccatgca gcgtgagagt gccggatatc tccatcgact gtgcccctgt
     8821 catccggatc ccttcagact tgaagacgat ctgtgggaac ttcgtggtgt cgagcatctg
     8881 cggccccttc atgatgtcga gacacatctg cggcgcggcg tccatctcga atgatgaagc
     8941 gtcgatggtc gtcaccacct tggaggcggg tagattacga gggtcgaacg tcaagtccac
     9001 gtcgaagcgg ctaaagcgcg tcgtgtaggt ggagaagccc atgtgactgg cacgcagcac
     9061 caagctggcg tgggacttat cgagtttgta cgcgcccgct gggggtgctg ccatcggcgc
     9121 cattgcaggc ggcccagccg actcggtcgc ttgcgacaac ccgctatgca aacccagaat
     9181 tgcagcaacc agcgcggcgg cgaggacgcg caactcggcc gaatttgtgc gcttcgtgca
     9241 aatcccgttg tgtacgggcg gcgtgttgaa atggatcatc atcggcggcg ttctccttgt
     9301 acctgattta tcaactattt tgcggccttg tctttcgtca caacgtgctt ggcgagtcta
     9361 ctcctggtct cgaccctcga gcgtcatggc tctctccctt atagctgccg ctcaatggct
     9421 gcgggaacga ggtatcttcg ggtcgtgcat cttgtgtcaa ggacgatggg ctaccttcca
     9481 aaccgacagg atggatgttt ttcttccgat tgtcggcagg cggacccctc gaacgtcctt
     9541 ggagcagaat ccgccaccac ctggagatat tcaatgaaag tcatggtttt tgttaaggcg
     9601 acaaaggaca gtgaagcggg tgtgcccccc gatccaaaga tgatggaggc catgggcaag
     9661 tataacgagg ccctcatcgc cgccggcatc ttgaaggagc aaatactggg cggactcaaa
     9721 ccaacccgct tcgccaaacg cgtgcatttc tcaggcaagc accgcacggt gatcgacggc
     9781 ccatttgtcg agacgaagga attggtcgca ggtttctggc tgtgggaggt ccagtccgtc
     9841 gacgaagccg tcgaatgggt gaagaagtgc cccaatccca tgctaacgga ctccgatgtc
     9901 gaaattcgtc cgctgtactc gctggaagac ataactcgct ggaagactta attcgctgga
     9961 atacatcgcg taactgctgg ctatttcggc gcgagcggac acagcccggt tgtcgggccg
    10021 aaggcgcggt cgtcaagaat agaccctgcg ccacatccaa gccaagcgaa tggacgtagt
    10081 ggatgaatac cagtaagcag cgccgccctt ggccgcgcat cggggcgacg caccctttgc
    10141 caccgggctg ccaacaccgg cgtatctacc cacctgacta ttgtcgcgcg cccggcgctg
    10201 cgcgaaccat gaactgcgaa gcacgtcgcg cgctccgagg tcgtgctgcg cgccgttcgc
    10261 cggtatcatc ggactacaat gccaatccca ccgcccgctg ctacattt
//
LOCUS       DATLVW010000383         2649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_426758, whole genome shotgun sequence.
ACCESSION   DATLVW010000383 DATLVW010000000
VERSION     DATLVW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426758"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VNN25_11505"
     CDS             complement(<1..1037)
                     /locus_tag="VNN25_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HXH92198.1"
                     /translation="MNVSTDSRTLPTPLLRTSAVRVAILFLLHVLVFTGSYLLAWMAR
                     FDFSVPPRYAAVMWSSLPFIVLVQLGVATGFGFFRGWWRYVGISDVLRIATGIVVALV
                     ATIALWYISAAVAFQPVVGVSRSVLLIDSMFALLAIFGARVLVRLTKDLTRADTTHEA
                     RRILIVGAGDAGEALAREIQHRPQLGMKIVAFVDDHRAKWRSQIRGIEVLGPISGVGA
                     IAEDTRADEVLLAIPSASGRRIREIIHHLSAAGLKFRTMPGLDQIASGKAQVTSLREV
                     NVDDLLRREQVVIGDTSTRDLFRGRKIIVTGAGGTIGSELAMQILNYEPSELHLVERS
                     EHALYNCCRRI"
     gene            1271..1963
                     /locus_tag="VNN25_11510"
     CDS             1271..1963
                     /locus_tag="VNN25_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92199.1"
                     /translation="MFFAENGPSLLEDEALALLDNPNCGATICQAIARTTHLTAFYAV
                     RFRLVQHRATPLTHAVKLVHYLFWPDLLRLSVDVKVPAQVRRAVETQLLFRVKKLSLG
                     EKIASAKRCSAALINVFLFDPDPKVFAALLINQRLREEDLVLFAGSESALPESLRLLA
                     ADQKWSFRYAIRRALVMNPSTPRSSAASQLRFLSQRDLCLIHEHPETSTYLRRCIERL
                     RERVFVRMGGQS"
     gene            2049..>2649
                     /locus_tag="VNN25_11515"
     CDS             2049..>2649
                     /locus_tag="VNN25_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH92200.1"
                     /translation="MPDQDPTPLQSELASFGEELRREREIRGISLKEIADATKISKRF
                     LEAIERNDHKTLPAPVFTRGFVREYARYLGLSSEEMVNRYNFAAVGDDRIEQSAHLER
                     LTTPQALPIPKKKAPQVSIPPAYARVDRNVYILILVVIALAAVSYWALKHRRARRTAF
                     YNCAAARSTQRGTRTGFRVGISRITARHYRSCTFRHKFRR"
BASE COUNT          521 a          833 c          793 g          502 t
ORIGIN      
        1 tcgatgcggc ggcagcagtt gtagagcgcg tgctcggatc gctccaccag gtgcagctcc
       61 gaaggttcgt agttgagaat ctgcatcgcc agctcggagc cgatggtgcc gccggcgccg
      121 gtgacaatga tcttgcggcc acggaagagg tcccgagtgg aggtatcgcc gatcaccacc
      181 tgctcgcggc gcaggaggtc gtcgacgttc acctcgcgaa gactggtgac ttgtgccttg
      241 ccgctggcga tctggtcgag gcctggcatg gtgcggaact tcagaccggc ggcggagagg
      301 tgatgaatga tttcgcgaat ccggcggccc gaggcgctcg gaatcgccag caacacctcg
      361 tcggcgcgcg tgtcttcggc gatggctcca accccggaaa tgggaccaag cacttcgatt
      421 ccgcgaatct gtgatcgcca tttggcccgg tgatcgtcga cgaaggcgac gattttcatc
      481 ccgagttgcg gtcggtgctg aatctcacgc gccagagctt cgccggcatc gcctgcgcca
      541 acgatgagga ttcgcctggc ctcgtgggtg gtgtctgcgc gcgtcagatc cttggtcaga
      601 cgaacgagca cgcgcgcccc gaagatcgcc agcagggcga acatcgagtc gatcagaagc
      661 acgctgcgtg agacgcctac gaccggctga aacgcgaccg ccgcgctgat gtaccagagc
      721 gcgatcgttg cgacgagggc gaccacgata ccggtcgcga tccgcaggac gtcgctgatg
      781 ccgacgtacc gccaccagcc tcgaaagaac ccgaatcccg ttgccacgcc gagttgcacc
      841 agcacgatga acgggaggct gctccacatg acggcggcgt aacgaggagg cacggaaaag
      901 tcgaaacgtg ccatccaggc caggagatag ctgcccgtga aaacaaggac atgcagcagg
      961 aaaagaatgg ccacccgtac agcggacgtg cgcaggagag gcgtgggaag cgtacggctg
     1021 tcagtcgaaa cgttcatcag gggagttggg gtgcggggag tcgggtgtgg ggggtgggag
     1081 aaatacatcg ccctgtacgg tgatcattta actcccactc cccacaccca actccccact
     1141 cctgtaagac accaatgtgt ttgcgccgat cataatcgaa agttattcta aaaacagcgg
     1201 cgcgccgcgc cggcagccta tgcagtattc cagccttgca ctccggaacc tttcacggga
     1261 agagctatcg gtgttcttcg ccgagaatgg tccgtcgctt ctggaggacg aggcactggc
     1321 gctgctggac aatcccaact gcggcgccac gatttgtcag gccatcgcca gaacaaccca
     1381 tctcacggcg ttttacgcgg tgcgttttcg tctggtgcag catcgcgcca cgccgctcac
     1441 gcatgcggtc aagctcgttc attacctctt ctggcccgat ttgctgcgcc tttcggtcga
     1501 cgtgaaggtt cccgcgcagg tgcggcgcgc tgtcgagacg cagctcctct ttcgcgtgaa
     1561 aaagctctcc ctcggcgaga agatcgcttc ggcgaagcga tgcagcgcgg ccctgatcaa
     1621 cgtcttcctc ttcgatcctg atccgaaggt ctttgcggcc ctgctgatca atcaacggct
     1681 tcgtgaggaa gatctggtgc tgtttgccgg ctcagagagc gcgttgccgg aatctctgcg
     1741 gctcctggcc gcagatcaga aatggtcatt tcgctacgca atccgcagag ccctggtcat
     1801 gaatccgtcg acgccgcgat cgtccgcagc gtcacaattg cggttcctct cgcagcggga
     1861 tctctgcctc attcacgaac atcctgaaac ctcaacgtac ctgcgccgct gcatcgaacg
     1921 gctgcgagag cgcgttttcg ttagaatggg gggacagagc tgagagatca gcggccagag
     1981 atcagagatc acgtgcccga gacccgtcct ccccgctaac cgccagcctc tgatttcttc
     2041 tcgcaactat gcctgatcag gatcccacgc ccctgcaatc ggagctcgcg tcgttcggcg
     2101 aggagcttcg gcgcgagcgt gagattcgcg gcatctcgct caaagagatc gccgatgcta
     2161 cgaagatcag caagcgcttt ctggaggcga tcgagcgcaa cgatcacaag acgctgcccg
     2221 cgccggtttt cacgcgcggt ttcgtgcgcg agtacgcccg ctacctcggc ctgagctcgg
     2281 aagagatggt caatcgatac aacttcgctg ccgtcggtga tgaccgcatc gagcagtcgg
     2341 cgcatctcga acggctcacg acgccccagg cgttgccgat ccccaaaaag aaggcgccgc
     2401 aggtcagcat tccgccggcc tacgcccgcg ttgaccgcaa cgtctacatc ctcatcctcg
     2461 ttgtgatcgc actcgccgcc gtcagctact gggcgctcaa acaccgccga gctcgccgca
     2521 ccgccttcta caactgcgcc gccgcccgct cgacacagcg aggaacgcga acaggtttcc
     2581 gcgtgggaat atctcgaatc actgctcgtc actatcgttc ttgcactttt cggcacaagt
     2641 ttcgtcgtt
//
LOCUS       DATLVW010000384         2448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_224970, whole genome shotgun sequence.
ACCESSION   DATLVW010000384 DATLVW010000000
VERSION     DATLVW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224970"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..513
                     /locus_tag="VNN25_11520"
     CDS             202..513
                     /locus_tag="VNN25_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92201.1"
                     /translation="MEFNTLENKMDEKQKSFLYNDNDATFDASSKRRMAVEVALDLIR
                     HEAMSGSSRNSVDFNLRHLSDFADYIQEALRGGEQEVLRGKEREALRGRERETSRGQQ
                     H"
     gene            1329..>2448
                     /locus_tag="VNN25_11525"
     CDS             1329..>2448
                     /locus_tag="VNN25_11525"
                     /inference="COORDINATES: protein motif:HMM:NF040140.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXH92202.1"
                     /translation="MEVKSIVAYQGPNIHAHFPVIRYTVDLGVLEEWPTGRLGQRFID
                     SLLGYLPGLAQHGCSYQSPGGLVRRMIEGDGTWLGHVMEHAAIELQKMAGSDVSFGRT
                     RSAGPDGHYDVIYEYHNEAVGRQAGDLAIALIEHLLPAELKPQTDTETQFDFEQKLDD
                     FLRDARRHEVGPSTAALMKAAADRAIPCTRMNADSLIRLGYGRFQKRIKATLTSETRQ
                     LAVDIASDKEETNRTLRDAGLPVPRQRKVYRACDAVVSAEQLGYPVVVKPLDCNHGQG
                     VSIKLTTPEQVELAYKRARKYSHAVLVESYIPGSDYRMLVVNDQLAAVARRVPAHVVG
                     DGLHTVEQLVEMANRDPRRGVGHENVLTRIELGDTAQSV"
BASE COUNT          545 a          693 c          733 g          477 t
ORIGIN      
        1 catgtcatgg agcacgtagc cgccaacctc cccaccgacg gtgccgttat cgatgtcacc
       61 gatagcgtgc acccgagcgc ggcgtgcggt tggcgcgaga tcgaatgaat gagttttcgc
      121 catgatgacg gcgacatgcc aggcggtcgc aagctgacct ggtgacaggg ctccagggcg
      181 ggtgcaagag gtctatcctt catggagttc aatactttgg agaacaaaat ggatgagaaa
      241 cagaaatctt tcctctataa cgataacgac gccacttttg atgcctcatc gaagcggagg
      301 atggccgtcg aagtcgccct ggacttgata cgtcatgaag caatgagcgg gtcttcacgc
      361 aattccgtgg atttcaatct gaggcacctc tcggactttg cagactacat tcaagaggcc
      421 ttgagaggtg gtgagcaaga agtcttgaga ggcaaggagc gagaggcctt gagaggcagg
      481 gagagggaaa cgtcgagagg ccagcagcat tagcggacgg tgcgcctcag gcacggcggg
      541 accgctgagg gccgctctga aagctacaat ggtcacgcag ggcgactcga ccagagtcgc
      601 ctttggccga tagaacaaac ctcaagaggg aacaaacctc aagaggacgc gacgggcaag
      661 atcggcgcgc gaaggtccgt tggctgggcg gagccgacgt tcgaacgttc ggatgaaagt
      721 gtaggcgaga cccaccgccg aacggccgtc tcacccagct ccatcgactg caaacctcgc
      781 tcaccgataa tccctggtcg caccatctgt accacctcca gcttgaggcc gacgtcaaat
      841 tgtcagccgg caccttcttg tcatctagct ttttcctcgt cgattcgcga ttgtcctcct
      901 atcgacttgt ccgaggaaat tagaccacca cattcctcga tagtgtgcgc tgcgcctgcc
      961 atccacactc cgccttcaaa cgtttgtgat cgccaactct cgaccggggc tgggtcaaaa
     1021 cccaaaattt tgaggttttt ccgatttgtc cgcctcggcg cggtgtcgaa cggggcaagc
     1081 gcctcgacac aaacgcatct aagccgccga ctccagccgc gaggccttgt agtgacagac
     1141 cagaaaatcg atgggtacag gttgctatat ccgtaaatgt ctctattttt tatagaccga
     1201 ctgcctagca gcgtcgacgg ctctgagtat caccagttga gtgaagtgtg cccgatgcgt
     1261 cttcttttgc gcctgcgctc ttcgtcactc gtgcatgaca cgatgtgata gctacaggag
     1321 gtcatacgat ggaagtcaag tcgatcgtcg cgtatcaagg ccccaacatt cacgctcatt
     1381 tcccggtcat ccgatacacc gtcgatctgg gcgtgctgga ggaatggccg accgggcggc
     1441 tcggtcagcg attcatagac agtttgctcg gttatctgcc gggcctcgca cagcatggct
     1501 gctcgtatca gtcccccggc ggactcgtgc ggcgcatgat cgaaggcgat ggcacgtggc
     1561 tcggccatgt catggagcat gctgccattg aactgcagaa gatggcgggt tcggacgtca
     1621 gctttggcag gacgcgatct gcgggtccgg atggccacta cgatgtcatc tacgaatacc
     1681 ataatgaagc cgtcggccgg caggccggag atctggcaat agcgctgatc gagcatctct
     1741 tgcctgccga attgaaaccg cagacggata ccgagacgca gttcgacttc gaacagaagc
     1801 tcgacgattt tctccgtgat gcgcgcaggc acgaggtagg gccgagcacg gcggcgttga
     1861 tgaaagccgc ggcggacagg gcgattccgt gtacgcggat gaacgcggac agcctgatac
     1921 gacttggcta tgggcgcttt cagaaacgga tcaaagccac gctcaccagc gagacgcgtc
     1981 agctcgcagt cgacatagcc tccgacaagg aagagacgaa ccggacgctg cgggacgccg
     2041 gcttgccggt gcctcgccag cgcaaggtct atcgcgcctg cgatgcggtc gtgagcgccg
     2101 aacagctcgg ctatccggtt gtcgtcaaac ccctggactg caatcatgga cagggcgtat
     2161 cgatcaagct cacgacgccg gagcaggtgg aactcgcata caagagggcg cggaagtaca
     2221 gccatgccgt actcgtcgaa agctatatcc ccgggtccga ctatcggatg ctggtggtca
     2281 acgatcaatt ggccgcagtg gccaggcgcg tgccggccca tgtcgtcggt gacggtttgc
     2341 atacggtcga gcagttagtg gaaatggcca atcgagatcc gcgtcgcggg gtaggccacg
     2401 aaaacgtact cacccgcatc gaactcggtg acacggcgca aagcgtac
//
LOCUS       DATLVW010000385         8370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_98513, whole genome shotgun sequence.
ACCESSION   DATLVW010000385 DATLVW010000000
VERSION     DATLVW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98513"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1490
                     /gene="rho"
                     /locus_tag="VNN25_11530"
     CDS             129..1490
                     /gene="rho"
                     /locus_tag="VNN25_11530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXH92203.1"
                     /translation="MTPDNRQPRKRRRRRRRAGAPGEASGDIAQGQPNGNIALPGDQQ
                     SFNDGAGGGAGRRRRNRSRRRGGKPQGYSGDAQQAPPIDLPPGELAPVSGVLWIKPNG
                     TGLLVQPSNNYVPQPGDAIVPRPVIERLHLQAGLLLSGSARRAGNNLEFVGLEQVEGM
                     ALEEYRESRRPFSELISIDPNEVFKLETEPERLTTRVIDLLSPLGKGQRCLVVAPPKA
                     GKTTLLKDIAHGLNLNHPEVTLFVLLVDERPEEVTDFRRSVAKGEVVASSSDETAENH
                     IQIAEVVLERARRLVEMKQDVVILCDSITRMSRAYNNEQRGSGKILSGGIDARTMEKP
                     RRFFGAARNAEEWGSLTIIATALVDTGSRMDEVIFQEFKGTGNTEIVLDRGLFERRIF
                     PAINIAQTGTRKEEKLLPPTVLPKTHTLRRALAGTDPLNAMKMLLERLQKFSSNDAFL
                     KSF"
     gene            1664..5032
                     /locus_tag="VNN25_11535"
     CDS             1664..5032
                     /locus_tag="VNN25_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH92204.1"
                     /translation="MSDRSLHSRGIPSFARKLMFVVSVVACITLAMPATIHAQVTTGN
                     IAGGVTDASGAALPGVTVEAIHVPTGTRYSAVTDSSGRFTIPNARVGGPYKVSGALEG
                     MKPETVSGVSVNLGSTTEVALKMSLNAVSESITVTAKPDDVINPNRTGSTSTVAEKQI
                     QDLPSVNRNLQDFARTNPYFVVDARDDSATLLQVAGRNNRYNNIQIDGAVNNDLFGLA
                     ASGTPGGQSNASPISLDAIEQLQLVVSPYDVRQGGFTGGGINAVTRSGSNKLSGSLFG
                     TKRNQKNVGKYRPVFVNADYVSGGSGTATDIAAQPIVNFDYSQYGGRLGGPIVQDKLF
                     FFVNAERDRRSKPNGTSADGSAGTNYTNTTQDAVCGGVSGCSAARLRQDLITKYSFDP
                     GGLGDIQQQTPADLLFGRLDFNATAGNALTLRNNYVKGATDVVANRGGTFFSYPTTQY
                     TQQNHTSSTVAQLNSVISTGSFNEARLGYQTIKDIRAPGANFPSIEIGGVNQSATLTV
                     GTERFSGANSLDQKITEITDDYTMLKGNHTIVIGTHNELFKFKNLFLSEFNGYYFYPR
                     LDDFENPSNCGQTITGRGKINCEYRLSFATGADPKRPTTFGAEQFGLYVNDQWHVSNN
                     LTVTAGLRADKPRFNDTPSFNPIVQTALGFNTATHPAEDIVLSPRIGFNWNPGGGTTQ
                     QVRGGIGVFAGRAPYVWVSNAYAGTGVEQIALACRTVDGCPVPGFNPDPANQPHSVAG
                     AAPVPLSVDLIDPHFKFPRVLRTTLGYDRELFFGIRGTVEVLYSKTQEDVYYQNLNDK
                     QTGTSPLDGRPTYSRVSTSVVDATFLTNTTKGSEKTGTIVLSRPFSNGITLSGDYAHQ
                     RAQSAFDATSSRAISNWRFQHNMGDIFTPALGTSAFQQKDRISLNGTYDFHTGPFGHT
                     FGLYFNAQSGRPYSLLLGADLAGLDVNRDTNASNDLLYVPGGTDKFILCPSNATAATA
                     TNPCGTRTALNPQLLQDYLTFAGINPNKARILNKYESFEPWSRKLDFHYGLTLPIKVM
                     HTEVDFDMLNLMHFISKSDGNVYFVSNQNVTPVNYLGQDPTTGKPIYRENNTTFVTGS
                     TTNRNFGSLTPGRQFSIADLSSRWQARLGFRITY"
     gene            5123..5416
                     /locus_tag="VNN25_11540"
     CDS             5123..5416
                     /locus_tag="VNN25_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXH92205.1"
                     /translation="MVKKVTFTLDDITIRRLNTVSDRLRKPKSEVVRDAIADYHDRAG
                     KLSEVERQRMLRVVRELVPTIPKRPLKEVENEIEAVRSARRAGGRGGVSRSSR"
     gene            5413..5784
                     /locus_tag="VNN25_11545"
     CDS             5413..5784
                     /locus_tag="VNN25_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH92206.1"
                     /translation="MIVVDTSALIDALTGPRRSLPRLLDFIESGERLIVPAVVLYEWL
                     RGPRLPEELHVCEELFPQDQAVVFGATEAYVAANLYRKLRRPRGREIDLTIAATAILR
                     DAALWTLNRDDFRDIESLPLV"
     gene            complement(5797..7164)
                     /gene="rimO"
                     /locus_tag="VNN25_11550"
     CDS             complement(5797..7164)
                     /gene="rimO"
                     /locus_tag="VNN25_11550"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXH92207.1"
                     /translation="MSIDVSINKPIRRVGMISLGCPKNLVDSEIMLGQLREESEVEIT
                     NDLEAADVVIVNTCGFIDAAKQESIDTILEVAERKGKGLQRLVVTGCMVMKYRADLQQ
                     AIPEIDAFVGLDSLEAITGAVTGVIDDAAPARRKMSVRLYEDLPRVLAQGTSHAYLKV
                     SEGCSNPCTFCAIPQMRGKFRSRAIASLVREAQALEAQGVKELCLVAQDTTRYGEDLG
                     IPNGLTSLVQTLLAETGFPWIRFLYAYPGSIDWSLFELMGREKRFASYCDIPLQHVSA
                     NVLKIMRRPGSPSDYREMVTRMRDLVPDLSLRTTFITGHPGETPHDFGELHEFVEWAQ
                     FDQMGAFVYSAEDATIAARMPDLPTRRTAERRREKLMEMQQEISRSRNEAKIGEIFDA
                     LITGVCDETEHLLEGRVIGQAPEIDGRLLINDGIELLPRDLPAFARVAITDAHPYDLV
                     GAVVG"
     gene            complement(7298..7714)
                     /locus_tag="VNN25_11555"
     CDS             complement(7298..7714)
                     /locus_tag="VNN25_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92208.1"
                     /translation="MDLNVDDATAIADFFLTLREEEEGGLHIEMMSRSKGHLAGFPAW
                     DHADRDLRHFTPDDIPLGEEEPFEDADEAWRIVIIARGKDVFVLEGDAPRGPLPRRYR
                     IPRDQYLTAWNELMVRFHPPLALEDVLGGGDDQPDA"
     gene            complement(7725..>8370)
                     /locus_tag="VNN25_11560"
     CDS             complement(7725..>8370)
                     /locus_tag="VNN25_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92209.1"
                     /translation="ILFPLSFQGPGAFGSQWTTESFLSSSGSAAYFRDPLPCAGCSST
                     FTLGTSRLTNDGNPWGHVLYALRGTTGSLELASRIRDTSRQAITAGTEVPVVRERDFR
                     ALLRFMNIPVDPKYRVTLRLWALGDYPQYSVGVDAVPALQNGISLTRITGTSMWFGSM
                     DVTSMIKLADGNPTNLLVTPWPNGGTNLLPAPPIWGMLSITNNDTQQVTIVSSQ"
BASE COUNT         1689 a         2777 c         2424 g         1480 t
ORIGIN      
        1 ctttcgtgcg acgatctcag taaggcatcc cgccgggcgt tccagagaat ggtcttctcc
       61 gcctgatcgt cggctttccg gccaaactcg aattcgagca atatctcagg gtcgtttatt
      121 gaacacgcat gacgccagac aatcgacaac cgcgtaagcg cagacggcgc agacggcgtg
      181 cgggtgcacc gggggaagcc tccggagaca tcgcgcaagg ccagcccaac ggcaacatcg
      241 ccctacccgg cgatcaacaa tcctttaacg atggagcggg cggaggtgcg ggccgcagac
      301 gccgcaaccg ttcgcgccgc cgcggcggga aaccgcaggg atacagcggc gacgcgcagc
      361 aggcgccgcc gatcgatctc ccgccgggcg agctcgctcc tgtctcggga gtcctctgga
      421 tcaagccgaa tggcaccgga cttctcgtgc agccgtcgaa caactatgtg ccgcaaccgg
      481 gcgacgccat cgtcccgcgg ccggtcatcg agcgattgca cctgcaggcg gggctcctcc
      541 tttcgggcag cgccagaagg gccggcaaca atctcgagtt cgtcggtctg gagcaggtcg
      601 aaggaatggc gctcgaggag taccgcgagt cgcgacgccc cttctccgaa ttgatctcga
      661 tcgatccgaa tgaagtgttc aagctcgaga cggagcctga acgtctgacc acgcgcgtca
      721 tcgatcttct ctctcccctc ggcaaagggc agcgctgcct cgtcgtcgct ccgccgaaag
      781 ccggtaagac gacgctgctg aaagacattg cgcacggact caatctcaat catcccgaag
      841 tgaccctctt cgttcttctc gttgatgagc gtcccgagga agtcacggac ttccgccgct
      901 ccgtggccaa gggcgaagtc gtggcctcga gctcggacga aaccgccgag aaccacattc
      961 agatcgccga ggtcgtgctc gaacgcgcac gccgcctcgt cgagatgaag caggacgtcg
     1021 tcatcctctg cgactccatc acccgcatgt cgcgcgcgta caacaacgaa cagcgcggct
     1081 cgggaaaaat cctctccggc ggcatcgacg cacggacgat ggagaaaccg cggcgtttct
     1141 tcggcgcggc acgcaacgcc gaggagtggg gatcgctgac gatcatcgcc acggcgctcg
     1201 tcgacaccgg ctcgcgcatg gacgaagtga tcttccagga gttcaaggga accgggaaca
     1261 cggaaatcgt gctcgaccgc ggcctcttcg agcggcgcat tttcccggcc atcaacatcg
     1321 cacaaacggg aacgcggaaa gaggagaaac ttctccctcc gaccgtactg ccgaaaacgc
     1381 acacgttgcg ccgtgcgctg gccggaaccg acccgctgaa tgcgatgaag atgctcctcg
     1441 aaaggctgca gaagttctcg tcgaacgacg ccttcttgaa gagcttctga ggcgaaaaaa
     1501 gccgcttcgg taaaacgttt ttttgatcca aaaaaccgtt ttcgcgggtc attcccgctt
     1561 tgctccaaaa tccctcaaat tcgctattcg ttttgaggca agtagtgtaa aaagacggct
     1621 acagtccacg ttcccccaac ccagtcacga caggaggatt tctatgtccg atcgcagtct
     1681 tcattcgcgc gggattccga gttttgcccg caaactcatg ttcgtcgtga gtgtcgtggc
     1741 gtgcatcacg ctcgcaatgc ccgcgacgat tcacgcacag gtcaccaccg gcaacatcgc
     1801 cggcggcgtg acggacgctt ccggcgccgc actacccgga gttaccgtgg aggcgattca
     1861 tgtccccacc ggaacccgtt actccgcggt gaccgattcg agcggccgtt tcacgattcc
     1921 gaacgcccgc gtcggcggcc cgtacaaagt cagcggcgcg ctcgagggca tgaagcccga
     1981 aaccgtctcc ggtgtctcgg tcaacctcgg ttccaccacc gaagtcgctt tgaagatgag
     2041 tctcaacgcg gtcagcgaat cgatcacggt cacggccaaa ccggacgacg tgatcaatcc
     2101 caaccgcacc ggctcgacct ccacggttgc ggagaaacag atccaggatc tgccgtcggt
     2161 caaccgcaac cttcaggact tcgcgcgcac gaatccctac ttcgtcgtcg atgcacgcga
     2221 cgactcggcg acgctcctcc aggtcgccgg ccgcaacaac cgctacaaca atatccagat
     2281 cgacggcgcc gtgaacaacg acctgttcgg tctggccgct tccggaactc cgggcgggca
     2341 gtcgaacgcc tcaccgattt cgctcgacgc catcgaacag ctccagcttg tcgtctcgcc
     2401 gtatgacgtg cgccagggcg gattcaccgg tggcggcatc aacgccgtca cgcgctcggg
     2461 aagcaacaag ctctcgggct cgctcttcgg caccaagcgc aaccagaaga acgtcggcaa
     2521 gtaccgccct gtttttgtga acgcagacta tgtcagcggc ggcagtggta cggcgaccga
     2581 cattgccgca cagccgatcg tcaacttcga ctacagccag tacggcgggc gcctcggcgg
     2641 accgatcgtg caggacaagc tcttcttctt cgtcaacgca gagcgcgacc gcagatccaa
     2701 accgaatggc acatccgccg acggatccgc agggacgaac tacaccaaca cgacgcagga
     2761 cgccgtctgt ggaggagttt cgggctgcag cgccgcacgc ctgcgccagg atctcatcac
     2821 caagtacagc ttcgatccag gtggcctcgg cgatatccag cagcagactc ccgccgatct
     2881 tctcttcggc cggctcgact tcaatgccac cgccggaaat gccctcacgc tgcgtaacaa
     2941 ctacgtgaag ggcgcgacgg acgtcgtcgc caaccgcggc ggcacattct tcagctatcc
     3001 gaccacgcaa tacacgcagc agaaccacac cagctccacg gtcgcacagc tcaacagcgt
     3061 gatctcgacc ggctcgttca acgaagctcg tctcggctac cagaccatca aagacatccg
     3121 cgcaccgggc gccaacttcc ccagcatcga gatcggcggc gtgaaccaga gcgccaccct
     3181 gaccgttgga accgagcgct tctcgggagc caactcgctc gatcagaaga ttactgagat
     3241 cactgacgac tacacgatgc tgaaaggcaa tcacacgatc gtcatcggca cgcacaacga
     3301 gctgttcaag ttcaagaacc tcttcctctc tgagttcaac ggttactact tctatccgag
     3361 gcttgacgat ttcgaaaacc cgtcgaactg cggacagacc atcaccggac gtggaaagat
     3421 caactgcgag taccgcctca gcttcgccac gggcgccgat ccgaaacgcc cgacgacgtt
     3481 cggcgccgag cagtttggcc tgtacgtcaa tgatcagtgg cacgtgagca acaatctcac
     3541 cgtcacggca ggccttcgcg ccgacaaacc gcggttcaac gacacgccgt cgttcaaccc
     3601 gatcgtgcag accgccctcg gattcaatac cgcaacccac cccgcggaag acatcgtcct
     3661 ctcgcctcgg atcgggttca actggaatcc aggcggcggc acaacccagc aggtacgcgg
     3721 tggcatcggc gtcttcgccg gccgcgcgcc gtatgtctgg gtgtccaacg cgtatgccgg
     3781 caccggcgtc gagcagattg cgctcgcttg ccgtacggtt gacggctgcc cggtcccggg
     3841 cttcaaccct gacccggcga atcaaccgca ctccgtcgcc ggcgccgcgc cggtaccgct
     3901 gtcggtcgat ctgatcgatc cgcacttcaa gttcccccgc gtcctccgca caacgctcgg
     3961 ttatgaccgc gagctcttct tcggtatccg cggaaccgtc gaggtgctct actcgaagac
     4021 gcaagaagac gtttactacc agaacctgaa cgacaagcag accggcacga gtcctctcga
     4081 cggccgccca acctattcgc gcgtcagcac gtcagtcgtc gacgccacct tcctcacgaa
     4141 tacaacgaag ggcagcgaaa agacgggaac catcgtgctc agccggccgt tctcgaacgg
     4201 catcacgctc tccggagact acgcgcacca gagggcgcag tcggccttcg acgcaacctc
     4261 gagccgtgcg atctcgaact ggcgcttcca gcacaacatg ggcgacatct tcacgcccgc
     4321 actcggcacg tccgccttcc agcagaagga ccgcatcagc ctgaatggaa cctacgattt
     4381 ccacacggga ccgttcggcc acaccttcgg cctctacttc aacgcccaga gcggccggcc
     4441 gtactcgctg cttctcggcg ccgacctggc cggcctcgac gtcaaccgcg acacgaacgc
     4501 cagcaacgat ctgctgtacg taccgggcgg tacggacaaa ttcatccttt gcccgtccaa
     4561 cgccaccgcg gcgacggcca ccaacccatg cggcacccgg acggcgctca acccccagct
     4621 cctgcaggac tacctcacct ttgccggcat caacccgaac aaggctcgga tcctcaacaa
     4681 gtacgagtcg ttcgagccgt ggtcgcgcaa gcttgatttc cactacgggc tgacgcttcc
     4741 gatcaaggtg atgcacaccg aagtcgactt cgacatgctc aacctgatgc acttcatcag
     4801 caagagcgat ggaaacgtct acttcgtatc gaaccagaac gtgactccgg tgaactacct
     4861 cggccaggat ccaacgaccg gcaagccgat ctaccgcgag aacaacacga cgttcgtcac
     4921 cggcagcacg actaaccgca acttcggatc gctgaccccg ggacgccagt tctcgatcgc
     4981 cgatctgtcg tcacgctggc aggcgcgcct cggattccgc atcacctact aactcttcac
     5041 gtcatcgcag tcacgaacgg gagggcttcg gccctcccgt ttttttgtct gccggatcac
     5101 ggtatggtag tattccgtcg ctatggtaaa gaaggttacc ttcacactcg atgacatcac
     5161 cattcgccgg ctgaacacgg tttccgaccg cctcaggaag cccaagagcg aagtggttcg
     5221 cgatgccatc gccgactatc acgaccgcgc cggcaagctc agcgaagtgg agcgccagcg
     5281 gatgctccgg gtggttcgcg agttggttcc caccatcccg aagcgtccgt tgaaagaagt
     5341 cgagaacgag attgaggcgg ttcgatccgc tcgccgcgcc ggcggacgcg gaggagtgag
     5401 caggagctca cgttgatcgt cgtcgacacg tccgcgctga tcgatgctct gacggggccg
     5461 cgccgttccc tcccccgcct tctggacttc atcgaatccg gagaaaggtt gatcgtgccg
     5521 gcagtcgtcc tctacgaatg gctgcgcggt ccgcgcctgc cggaagagct ccatgtctgc
     5581 gaagagcttt ttcctcagga tcaggctgtg gttttcggag caaccgaagc gtacgtggcg
     5641 gccaatctat atcgcaagct tcgccgcccg cgcgggcggg agatcgatct tacgatcgcg
     5701 gccacggcca tcctccgcga cgcggcattg tggacgctga atcgcgacga ctttcgcgat
     5761 atcgaaagcc tgccgctggt ctgaccgcgg cgtccgctag ccgacgaccg cgccgacgag
     5821 atcgtacgga tgcgcatccg tgatcgccac gcgcgcgaac gccggcaggt cccgcggcag
     5881 cagctcgatg ccgtcgttga tcagcagccg tccatcgatc tcaggcgcct gaccgatcac
     5941 gcggccttcg agcaagtgct ccgtctcgtc gcaaacgccg gtgatcagcg cgtcgaatat
     6001 ctcgccgatc ttcgcttcgt tgcgcgagcg cgagatctcc tgctgcatct ccatcaactt
     6061 ctcgcggcgg cgttcggccg ttctgcgcgt cggcagatcg ggcatccgcg cggcgatcgt
     6121 cgcatcttcg gccgagtaga cgaacgcgcc catctgatcg aactgcgccc attccacgaa
     6181 ctcgtgcagc tcgccgaaat cgtgcggcgt ctcgccggga tgcccggtga tgaacgtcgt
     6241 gcgcagcgac aggtcgggca ccagatcgcg catgcgcgtc accatctcgc ggtagtcgga
     6301 cggcgatccg ggacgacgca tgatcttcaa aacgttcgcc gagacgtgct gcagcgggat
     6361 gtcgcaataa gaggcgaacc gcttctcgcg ccccatcaac tcgaagagcg accagtcgat
     6421 cgagcccgga tacgcgtaca ggaaccgaat ccacgggaag ccggtctcgg cgagcagcgt
     6481 ctgtacgagc gaggtcagac cgttcgggat gccgaggtct tcgccgtagc gcgtggtgtc
     6541 ctgggcgacg agacagagct ctttcacccc ctgcgcttcg agcgcctgcg cctcgcgaac
     6601 cagcgacgcg atcgcacggc tgcggaactt gccgcgcatc tgcggaatcg cgcagaacgt
     6661 gcaggggttc gagcagccct cggagacttt cagataggcg tgcgatgtcc cctgcgcgag
     6721 cacgcgcggg agatcttcgt acaaccggac cgacatcttt ctgcgcgccg gtgcggcatc
     6781 gtcgatgacg ccggtcaccg cacccgtaat cgcttcgagc gaatcgagtc cgacgaacgc
     6841 atcgatctcc ggaatggcct gctgcagatc ggcgcggtac ttcatgacca tgcaaccggt
     6901 cacgacgagc cgctgcaggc ctttgccctt ccgttcggca acctcgagga tcgtgtcgat
     6961 cgactcctgc ttggccgcgt cgatgaaacc gcaggtattg acgatgacga cgtccgccgc
     7021 ttcgagatca ttcgtgatct cgacctcgga ctcctcacgc aactgcccca gcatgatctc
     7081 gctgtcgacc agattcttcg gacagccgag ggagatcatg ccgacgcggc ggatgggttt
     7141 gttgatggaa acgtcaatgc tcatggacag gcgttgaaca gggaggggtg caccggtatt
     7201 ctgcggcgcc gtgtctacgg aatcattcct ccttcgcgct gccactcgcg ccgcaccgac
     7261 gcccgatccg gcgctcgccg gacagcgcct cgtagactca cgcgtccggc tgatcgtccc
     7321 cgccgcccag cacatcctcc agcgccagcg gtgggtggaa gcggaccatg agctcgttcc
     7381 aggcggtgag gtattgatcg cgcgggatgc ggtagcggcg cgggagcggg ccgcgtggag
     7441 catcgccttc caggacgaag acgtccttgc cgcgggcgat gatgacgatg cgccaagcct
     7501 cgtcggcgtc ttcgaacggc tcttcttcgc cgaggggaat gtcgtcggga gtgaagtgac
     7561 ggagatcgcg gtcagcgtgg tcccaagccg ggaagccggc cagatgaccc ttcgaccggc
     7621 tcatcatctc gatgtggagg ccgccctctt cctcttcgcg aagggttaga aaaaaatcag
     7681 cgatcgccgt tgcgtcgtcg acgttgagat ccatatgtca aatcctattg cgatgaaacg
     7741 atcgttacct gttgcgtgtc gttgttcgtg atgctcaaca ttccccagat tggcggcgca
     7801 gggagcagat tcgtcccacc gttgggccac ggggtgacga gcaggttggt cggattcccg
     7861 tctgccaact tgatcatcga ggtgacgtcc atcgagccaa accacatcga cgttcctgtg
     7921 attctcgtca gcgagattcc gttctgcaga gctggaaccg cgtcgacgcc gacgctgtac
     7981 tgaggataat cgccgagggc ccagaggcgc agggtgacgc ggtacttcgg atccaccggg
     8041 atattcatga agcgaagcag cgcgcgaaaa tccctctcgc gcacgaccgg aacctcggtg
     8101 cccgcggtga tcgcctgacg cgaggtatcg cgtatgcgcg atgcgagctc gagcgaaccc
     8161 gtcgtaccac gcagggcata cagcacatgc ccccacggat tgccatcgtt ggtcagccgg
     8221 ctggtgccga gggtgaatgt ggaagaacag ccggcgcacg gcagcgggtc tcgaaaatac
     8281 gccgcagatc cgctggagct cagaaaactc tcggtggtcc attgggagcc gaacgctccc
     8341 ggtccttgaa acgagagcgg gaagaggatc
//
LOCUS       DATLVW010000386        20772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_426849, whole genome shotgun sequence.
ACCESSION   DATLVW010000386 DATLVW010000000
VERSION     DATLVW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20772
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426849"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..998)
                     /locus_tag="VNN25_11565"
     CDS             complement(84..998)
                     /locus_tag="VNN25_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXH92210.1"
                     /translation="MQEILQNLLNGIAVGAIYALIALGYTMVYGILKLINFAHGDVFM
                     LGAFVGFYAGGFAGRHLQTHGGPLGWVYAGLVMLAAMVVCGLFGFLNERIAYRPLRSA
                     PRIASLITAIGVSFLLEYGGQFVFGADPKFFPTLVEKHLISFGGVTTTNYQMIVLVVA
                     VIFMGLLQYIIYGTKFGRAMRAVSFNFETASLMGIPTDRIISMTFIIGSMLAAVAGIL
                     YGLSYPKIDPLMGIMPGLKAFIAAVLGGIGNVPGAVIGGLIIGIIESFVGGSHFSNYR
                     DAIAFIILIAILLFRPSGLFGKYQAEKV"
     gene            complement(1365..2618)
                     /locus_tag="VNN25_11570"
     CDS             complement(1365..2618)
                     /locus_tag="VNN25_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH92211.1"
                     /translation="MRKNLLILISIAVLSFGLLGCPPKENGGNTTTAAGSGEILIGEY
                     GSMTGAQATFGQSTHNGIMMAADEINAAGGINGRKIKVLSEDDQSKQEEAANAVTKLI
                     SQNNVLAILGEVASSASIAAAPICQSNKVPMITPSSTNDEVTRKGDYIFRICFTDSYQ
                     GEYQAVFADQWATANNKPKSVAMLTDVKSDYSQGLGKVFTAKFTSLGGKMVGTQSYAN
                     GDSDFRSQLTAIKATNPSLLYVPGYYTDIGQIAIQARDLGITCPLLGGDGWESPKLIE
                     IGGKALEGCFYTNHYFYADPAPVVANFVQKYKERYGQTPDALAALGYDDMKTLADAMK
                     RAGKLDGPSLRDAIAQTKGLTGVTGTITIGPERNAMGKKLVIEEIKNGQLTLAATIDP
                     ATGGKVEMNTGAAPAAATPATATKK"
     gene            complement(2778..3605)
                     /locus_tag="VNN25_11575"
     CDS             complement(2778..3605)
                     /locus_tag="VNN25_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXH92212.1"
                     /translation="MRFRPLRFCAILILGFASTVSAATDPILPHQTFTIDSKLLGETR
                     RINVYTPPGYEKSNSRYPVIYMPDGGVDEDFPHVTTDIDSAIRAGEMRPVIVVGIENT
                     ERRRDMTGPTQVESDRKIAIHVGGSASFRAFIRDELMPVIRKRFRGNGERAIVGESLA
                     GLFIIETFFEEPKLFDTYIALSPSLWWNDQALVHRASKWLRSRPRLAATLYLATGGDD
                     DRGDTVKALAAILRSDAPKRLTWYYEPRPDLQHSTIYKGASPGVFRKLFPPRRSDSG"
     gene            complement(3712..3945)
                     /locus_tag="VNN25_11580"
     CDS             complement(3712..3945)
                     /locus_tag="VNN25_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92213.1"
                     /translation="MSTKKATKPASDEMRPEYDLRGGVRGKFFAEYQKGTNLVLLDPD
                     VAKEFRDSEAVNRALRQFVEEHPSAKAPKRRAG"
     gene            complement(3923..4198)
                     /locus_tag="VNN25_11585"
     CDS             complement(3923..4198)
                     /locus_tag="VNN25_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXH92214.1"
                     /translation="MQFEWDPAKERRNRILHRISFIEAATVFGDPLQWTIEDPDHSTG
                     EARYLTTGFSNRQRLVIVSHTYRFQRVRIISARLATTTERYVYEEGN"
     gene            4290..4646
                     /locus_tag="VNN25_11590"
     CDS             4290..4646
                     /locus_tag="VNN25_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92215.1"
                     /translation="MMITAALMFTISAATSTETAPLPPPRLCLGAPGSDLMAGRCVDA
                     AKNEIDVVPDGKTRGWIWIDSGRKTIAFGTLAPDQSKIIIDASGRWKHRRSASPSTRA
                     ASTPLQTLRCCPARTM"
     gene            4730..5221
                     /locus_tag="VNN25_11595"
     CDS             4730..5221
                     /locus_tag="VNN25_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92216.1"
                     /translation="MGKIDEVLAEMLQERERLAADLARVDRVIAALEEGVTLVGDGET
                     GVANVPPGAAATTVARAAQTNLPRRIGPYTLLDVYEATAHYLSTVDTPKNSTEIAAAL
                     RVGGFKTRSRNFVNTVRVMLRRKDGRSTGISVTKDGKRWFLKRKAVKRRPPKTSAGSD
                     GAS"
     gene            complement(5700..7169)
                     /locus_tag="VNN25_11600"
     CDS             complement(5700..7169)
                     /locus_tag="VNN25_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXH92217.1"
                     /translation="MTAIANDSTIQNKLFINGEFVDAQSGQTFATANPATEEKIADVA
                     SAGEADVDAAVRAARAQMEPGSAWQKMKPRDRAKILWRIADMLTARAGEIGRIETIDN
                     GKPIFESEFVDTPAAAECLYYFAGWSGKVTGETIPIADNAFTYTLREPVGVVGAITPW
                     NFPLMLAVWKIAPALACGNTVVIKPASNTSLSLLKFAEYAKECGLPPGVLNVVPGRGS
                     VVGNALVDHPAVDAIAFTGSTEVGKGLMARAAKTLKKVSLELGGKSPNIVFADADLEA
                     AARGALNAIFYGKGEVCAAGSRLLVEEGAHDELMSKITERANKMVAADPLHPKTRLGA
                     LVSKEQMETVLSYIEAGKSDGAKLVAGGERTDIGTGKGYFVKPTIFDDVEVGHRIARE
                     EIFGPVLATIRFKDAEDAVAKGNATVYGLAAAVWTRDISKAHKIARSIKAGTVWINSY
                     NLYDPALPFGGFKESGFGRDQGRDALEKYTQTKSVWVNL"
     gene            complement(7337..8446)
                     /locus_tag="VNN25_11605"
     CDS             complement(7337..8446)
                     /locus_tag="VNN25_11605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH92218.1"
                     /translation="MRYRNLHGTNLQLSELGFGTWTLTTGWWGVYTDDEARRLISIAI
                     DAGINYIDTADAYGNGRGETMLAPLLAEHPEVIVGTKFGYDFYNNPERPRGQRELPQD
                     MSPKFVRFAIEQSLKRLGVDHVAIYQPHNPRVVTLMEDEHWETLESLKSEGKILSYGP
                     SLGPAIGWRDEGIYANAVRHAPVTQMIYNVLEQDPGREFILAAEKGPSYAGVPESMRG
                     ELGKKVCTPSSAFDASFRAWKADPDPQFLIRVTHSSGMLEGKYTLDTKFDETDHRSHR
                     PRSWMVEGLQKVDRLESLRKERGVTLGQFALLWLYAHKSMLSALPNIYDEEQIREFAA
                     ASDHAPLTDTEMERIQEMYVRNFDITPYVEEAVTA"
     gene            complement(8484..10583)
                     /locus_tag="VNN25_11610"
     CDS             complement(8484..10583)
                     /locus_tag="VNN25_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92219.1"
                     /translation="MHRHLLAAVIILSLSAFSVSATELEPRTIGPTAGNVSFVSSAFS
                     GGKFLTVWRQLFMPTVGIWGAFSDAAGKPLGPSFAIVPSTNAQYLNLFPSGSGFVLVW
                     DDANRSSQLCQIDGNGRVTGQRTIGPSGLTRAYAMPGRIVFSRSDGSTIVDFEGRPIR
                     ELAERAFIAAGHNSFFAVTTTADLMLTRYSGDGQSLQQWLLKPDFCHTLSTCWFVAAV
                     RELDSGSILVVVASNDHRIETLILAPDGSVSAPVVITMPLLVLSRASVQQSGTHLWLF
                     LAGVQDGGTARIEATELDNKGMALGPVMTVIDSLPETEQVVFAEGAGLVFAATSPTTA
                     GRQVQTFVFRTLFAPLGPEIISTTASIQADAQIASNGSDFLAAWKDTDGTRGSRWHWA
                     PVDAAGRPSGAVLEGDIAIVRKSLVSNGLDYLMLLVSSNGLIARRLDFDGSILGDTPI
                     FGSFVNLSGAAAVATANGYVIVWSADYTMYSVTLTSTGVAGPLRVLAPSVPSLPGRNL
                     RFWLSPVLAWDGSAILLTAVYEQDIVFVQTGADTTQTPYAMLLTSDGVLQGGSETPLD
                     PAFIAPSAAASSGTEFLVAGSEIALVHHNGTKLQVTYPIHNSLNAGVVWSGSAYAIAR
                     LVQGTAMVTLVARDGSILGTTFRRGPEDLDVAADAAGRLVISGVESDGRGPLRAVAYS
                     GSELQPLPIGRSRAAGH"
     gene            complement(10631..11329)
                     /locus_tag="VNN25_11615"
     CDS             complement(10631..11329)
                     /locus_tag="VNN25_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92220.1"
                     /translation="MARIFLLTATITFLSAQAMAQTGSCSAGIQDPPVFALVTGPVRV
                     TFRDTVNALLSEPSVTIAGNEITVVQSEFETLVAPPVSCNSQSVLLGDLPSGSYTLTW
                     KYQSLSRSPLETFPFAFTLQDASTCVPGLTIQPQSPAAGQPVSILYTATFRGFLQTPD
                     VAIDGNQITIDQPAVIADPAFAGHVPCARGVVQFKALQVGYYMVTVRSNSGAPMSDGF
                     VVSPAVRARAVRGH"
     gene            complement(11405..12205)
                     /locus_tag="VNN25_11620"
     CDS             complement(11405..12205)
                     /locus_tag="VNN25_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXH92221.1"
                     /translation="MSGNLIDYRVEKGVAILELNDPPANTYTYEMNKELDTCILNARM
                     DDDAYVIVLRGAGDKFFSAGANIKMLSEVTPQFKYYFCLHANETLNRLEQTPKLVIAA
                     LNGHTVGGGLEIAMAADIRIALKGAGKVGLPEVTLGVLPGTGGTQRLSRLVGKTKSIE
                     LMVEGGLFEFEEALRLGVINYLWPAEENGQKWWDRVMAYAQSFCPPNKASRAVGRIKR
                     SVQSGWEVPFESALAIERELQQQLFQSEDAKEGLSAYNQKRKPQFAGK"
     gene            12561..12812
                     /locus_tag="VNN25_11625"
     CDS             12561..12812
                     /locus_tag="VNN25_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXH92222.1"
                     /translation="MNILGWLLFGLVVGAIAKFLMPGRDPGGWIVTILLGIAGSFVGG
                     FLASMLGHNEQTAGWIGSIIGAIVLLFIYRLIVGRRAVV"
     gene            13067..14635
                     /locus_tag="VNN25_11630"
     CDS             13067..14635
                     /locus_tag="VNN25_11630"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXH92223.1"
                     /translation="MHTTTKRILSTIAIIIASVAFGVVLSADLGFMKASNAQSSAVPA
                     TLSNAPVPTVTIPSFADVAARVMPAVVSIRSTEVIKTSDLRKGTPFGGDPFDFFFPDP
                     RGNGNGQRSPHGRVPGGDDDEQKQVAGGSGFIISPDGFIMTNNHVIDGARTVEVHMGA
                     DENGNGGRTLEAKIIGRDPATDIALLKIDAGQALPYVPLGDSDRIRKGDWAIAVGNPY
                     QFENTLTVGVISAKGRSLGLSKETSSFENFIQTDAAINFGNSGGPLMNVNGEVIGINT
                     AIRGGGAQGLGFATPINTAKRLLSQLKQGKVTRGYLGMGIIDVSEDVKNSFNLSETRG
                     ALVQTIEPGKPAERAGLQPGDIIVEADGHPINNNRQLIDYISYQPVGNKINLTVIRDG
                     KRKTLTASTIERTLDGDKPDVTTPDEAAPAHNKLGMSVQELTPQLRQQYGQADTVSGV
                     VVTSVKEVSPAGDVLSEGDVISEVQGEKISNLAQFRAAIEHLKSGQTARIYVTSPAGR
                     GGAAISGYRFIHVP"
     gene            14853..15968
                     /gene="ribD"
                     /locus_tag="VNN25_11635"
     CDS             14853..15968
                     /gene="ribD"
                     /locus_tag="VNN25_11635"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009441233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXH92224.1"
                     /translation="MEDLMRRALDLAARGRYSVSPNPMVGCVIVRDGNVIGEGWHHRA
                     GEPHAEIEALRGCDARGATMIVTLEPCAHHGRTPPCVDAIVDAGVARVVVAMRDPHDA
                     AGGGIEKLREAGIEVTTGVLEPEARRLNETFIHAVTHRRPFVLLKAGMTLDGKLATVT
                     GESQWITSEASRERSLALREEYDAILVGGGTVRADNPRLTRRLGLNTSAAGWTRVILD
                     RSSVVSLDATVLTDGGPTIHITRDADLDELLAGLYGQGIHSVIVEGGSTVLSEFIRRG
                     MWQKMILFVAPMVVEGATAPSIFRGEGIAKLTDAPRFRFDRVETVGEDLMITLYSGGG
                     ELGVGSGSEPATPVRSTAHPSTPNTSSPTPHPPKPTP"
     gene            15968..16564
                     /locus_tag="VNN25_11640"
     CDS             15968..16564
                     /locus_tag="VNN25_11640"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017975964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXH92225.1"
                     /translation="MFTGIIHRTGIIESLVTNESGAQLRLRTEDAEPFTRGESLAVNG
                     VCLTVLPQDDGSLLTDVSNETLARTTLGALVRGARVNVERAMALGDRLGGHLVQGHVD
                     GVGTLQSIKREGAFAVYRWSHPAEYAALIVDKGSIAVDGVSLTVVEPDGATFGAALIP
                     ETLSRTNLGDAAEGGRVNLELDMLAKYVQSLVTPYLRR"
     gene            16588..17631
                     /locus_tag="VNN25_11645"
     CDS             16588..17631
                     /locus_tag="VNN25_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92226.1"
                     /translation="MIVYKKTQHLKLTRAHVAEPPYWAATTIAPYSPRRAAPIAIDYI
                     DLRASCADRFEVAVCENVRDDLERTTTRHPLHAPVMIDATEFAEVIFRRGQEALQFCA
                     EQQVAALHLVSTRGELPAEVQDDAVIAVAPWPLEFPRIEMLFAEAKERGLRWGVVVPI
                     IYPVTTDLTALAELAELAKKYAPSFFAGVPVELDATARNGLARSLTGDDQNDTYEMLF
                     HADLGPIRLATERHIAALAGEINAEDFVIPPRWDRHTNWNGAVLLTLLANRILAMNRD
                     EDLAWRLTRSAKVIAQLEKPIERVAQAASLGIIDSLDEISIDLLTEWLETGRSSFAGH
                     IEKQWRLRRDSGM"
     gene            17766..18410
                     /locus_tag="VNN25_11650"
     CDS             17766..18410
                     /locus_tag="VNN25_11650"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HXH92227.1"
                     /translation="MTRRRQILTLLGATAFIVAIAAATVWFLVRRIDQAVVSAVTSKE
                     ETVDLGTLVTRVRDLNRLETATMRVTSISTVKQTYDLIPNALAGDELTLYATGDVIAG
                     VDLSQLKQEDVRREPDGTIVVRLPPPQLLVSRIDNRTTRVTGRRTGVFRRADIGMEGR
                     ARQYAEQNIRNEAVHKGILPLAQQNAEARIAGLLHTLGATKVRFVGIEGNAGAR"
     gene            complement(18515..19279)
                     /locus_tag="VNN25_11655"
     CDS             complement(18515..19279)
                     /locus_tag="VNN25_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HXH92228.1"
                     /translation="MLVALTRAVPQAITRCELTHIDRNPIDVDIARKQHQAYEAALTD
                     AGCRIERLPAADELPDSVFVEDTAIVLDEIAIITRPGAESRRPETTSTAAALRNYREL
                     VFIEPPAILDGGDVLCIGRKIFVGQSTRTNAEGFRQLSAALIPFGYSVRAVTPRGCLH
                     LKSAVTAFSDDGVVLNPEWVDASLFDGMQVIAVDPSEPSAANVLRVGDVVLCAAAFPR
                     TAQRLRDAGVNVRTVDVSELAKAEGALTCCSIVVAS"
     gene            complement(19382..>20772)
                     /gene="trpE"
                     /locus_tag="VNN25_11660"
     CDS             complement(19382..>20772)
                     /gene="trpE"
                     /locus_tag="VNN25_11660"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXH92229.1"
                     /translation="IVPIARELSADAITPVAAFAALSGDGEAFLFESVERGENVGRYS
                     FVGFEPRRSLRFDESTPDPVGVLNNELQPLRVYGEESLPPFFGGAVGFFGYSVAGWIE
                     RIPDSYQNETGIPDARLIFFDNVVVFDHVKQRLYVIASLFTSDAHASFEEANARLDSA
                     IERLRAARVELLAFPEHVVDVAFTPNIPRPDFEEVVRAAKEEITAGEVFQIVLSQSWS
                     TPFPESEALTLYRVLRSVNPSPYMFLLRTPECTLVGASPEMLVRVDGTRAETRPIAGT
                     RPRGATEEEDAELERALVADPKEQAEHLMLVDLGRNDLGRVCVSGSVSVSDFCHIERY
                     SHVMHLVTNVEGTLRDDRSPVDLFLSCFPAGTLTGAPKIRAMQLIDEHETTRRGPYGG
                     AVAYFGFSGNMDSCITIRTIVLANGKASVQAGAGIVYDSDPAREYEETMSKSAALKLA
                     IRIATTVLNKPT"
BASE COUNT         3964 a         6395 c         6622 g         3791 t
ORIGIN      
        1 aaaactagaa ggcagaaagc agaaggcaga agtgcgattg cacttgttca cctctgccct
       61 ctgacttctg acttctgact tcatcagact ttctccgcct gatactttcc gaacagcccg
      121 gacgggcgga acagaagaat cgcaatcagg atgatgaagg cgatggcgtc gcggtagttc
      181 gagaaatggc tgccgccgac gaaggactcg atgatgccga tgatcagccc tccgatcacg
      241 gcgcctggca cgttgccgat gccgccgagg acggcggcga tgaacgcttt gagtccgggc
      301 atgatgccca tcagcggatc gatcttcgga tacgacaggc cgtagagaat gccggccacc
      361 gccgcgagca tcgagccgat gatgaaagtc atcgagatga tgcggtcggt cgggatgccc
      421 atcagcgagg cggtctcgaa gttgaacgag acggcccgca tggcccggcc gaacttcgtg
      481 ccgtaaatga tgtattgcag gaggcccatg aagatgaccg cgacgacgag cacgatcatc
      541 tggtagttcg tcgtcgtcac gccgccgaag ctgatgaggt gcttctccac gagcgtcggg
      601 aagaacttcg ggtcggcacc gaagacgaac tgaccgccgt actcgagcag gaacgagacg
      661 ccgatcgccg tgatcagcga tgcgatgcgc ggtgcgctgc gcaggggccg gtaggcgatg
      721 cgctcgttga gaaaaccgaa cagaccgcag acgaccatcg ccgcgagcat gaccagtccg
      781 gcgtagaccc agccgagggg gccgccgtgc gtctggagat gccgcccggc gaagccgccc
      841 gcatagaagc cgacgaaggc gccgagcatg aagacgtcgc cgtgagcgaa gttgatgagc
      901 ttcaggatgc cgtacaccat cgtgtagccg agggcgatca gcgcgtagat cgcgccgacg
      961 gcaattccgt tgagcagatt ctggaggatc tcctgcattc agtggccgct atggtgtcgg
     1021 gtgtcgggtg tcgggtgtcg ggtgtcgggt gtcgggtgtc gggtgtcggc gagacggtaa
     1081 ccgaaatctg actaacgtca agacttgtgc tcgtcgttac aaaaaaagat ccggcgaacg
     1141 ctacccgccg gaccgtgtcc tgtaaagata aagtttggtg tcgggtgtgg ggggtggggg
     1201 tcgggggtcg gagaaaaaag cgcgcgtgtt actacgctgc gtgcgccggt tgcgggaggc
     1261 ggaagccgga gaaacctccg acacccgacc cccgataacc gactcccgcg ccggaagccg
     1321 gagaaatctc cgacctccgg ctcccgataa ccgacgcccg aatcctactt tttcgtcgcc
     1381 gtcgccggcg tcgctgccgc gggcgccgct ccggtgttca tctcgacttt gccgcccgtg
     1441 gccggatcga tcgtcgctgc cagggtgagc tggccgttct tgatctcttc gatgacgagc
     1501 ttcttgccca tggcgttgcg ctccgggccg atcgtgatcg tgccggtgac gccggtgagg
     1561 cctttggtct gggcgatggc gtcacggagc gacgggccat ccagcttgcc ggcgcgcttc
     1621 atcgcatcgg ccagcgtctt catgtcgtcg tagccgagtg ctgccagcgc gtcgggggtc
     1681 tgcccgtagc gctctttgta cttctgcacg aagttggcga cgaccggtgc ggggtcggcg
     1741 tagaagtagt ggttcgtgta gaagcagccc tcgagggcct tgccgccgat ctcgatcagc
     1801 ttcggcgatt cccagccgtc gccgccgaga agcgggcagg tgatgccgag gtcgcgcgcc
     1861 tggatggcga tctgaccgat gtcggtgtaa tagcccggga cgtagagcag cgacgggttg
     1921 gtcgctttga tcgcggtgag ctgcgaacgg aaatcgctgt cgccgttcgc gtagctctgc
     1981 gtgccgacca tcttcccgcc gagcgatgtg aacttggccg tgaagacctt gcccagtccc
     2041 tgcgagtagt cgctcttgac gtcggtgagc atggccacgc tcttcggctt gttgttcgcg
     2101 gtggcccact gatcggcgaa gacggcctgg tattcgccct gatagctgtc agtgaagcag
     2161 atgcggaaga tgtagtcgcc cttgcgggtg acctcgtcgt tggtcgacga cggggtgatc
     2221 atcggcacct tgttcgactg gcagatcggc gccgcggcga tggaggcgga ggacgccact
     2281 tcgccgagga tggccaggac gttgttctgc gagatcagct tggtgacggc gttggccgcc
     2341 tcttcctgct tggactgatc gtcctcgctg aggactttga tcttgcgtcc gttgatgccg
     2401 ccggcggcgt tgatctcgtc ggcagccatc atgatgccgt tgtgcgtcga ctggccgaag
     2461 gtagcctgcg ctccggtcat cgagccgtat tcgccgatga ggatctcgcc gctaccggca
     2521 gccgtggtgg tgttcccgcc gttttccttc ggcggacagc cgagcagtcc gaacgacagc
     2581 acggcgatgg aaatgagaat gagtaagttc ttgcgcatag gtctcctctt caaagagttg
     2641 cggattgtac agcagggacc gttcctggaa agtggatgtg gtcgccgaag caaaggcaac
     2701 cacagagaca cagagaacac agaggggatc tctgtgacct ctgtgtctct gtggtgatcg
     2761 gttttcaagt tgaccaacta cccagaatcg gaacgcctcg gcgggaaaag cttgcggaac
     2821 acgccgggcg aggctccctt gtaaatcgtg ctgtgttgga ggtcgggccg aggctcgtag
     2881 taccacgtca gtctcttcgg ggcatcggaa cgaaggatcg cggccagcgc cttgactgtg
     2941 tcgccgcggt cgtcgtcgcc gccggtggca agatacagag tcgcggccag cctgggccgt
     3001 gatcgcaacc acttgctggc gcggtgcacg agggcctgat cgttccacca gaggctgggg
     3061 ctgagagcga tgtaggtatc gaacaacttc ggctcctcga agaaggtctc gatgatgaag
     3121 agtcccgcaa gcgactcgcc gacgatggcc ctctcgccat ttccgcggaa gcgcttgcgg
     3181 atcaccggca tgagctcgtc gcggatgaag gcgcggaagc tggctgagcc gccgacgtgg
     3241 atcgcgatct tcctgtccga ctcgacctgc gtcggcccgg tcatgtcgcg gcggcgctcg
     3301 gtattctcga taccaacgac gatgaccggc cgcatttcac cggcgcgaat ggcgctgtcg
     3361 atgtcggtgg tgacgtgcgg gaagtcctcg tccacgccgc cgtccggcat atagatcacc
     3421 gggtagcgtg agttggactt ctcgtaaccc ggcggcgtgt agacgttgat gcgcctcgtt
     3481 tcgccgagca acttcgagtc gatcgtgaag gtctggtgcg ggaggattgg atcggtggcg
     3541 gcggaaaccg tggacgcgaa tcccagaatc aggattgcgc agaatctgag tggtcgaaac
     3601 ctcatgtttg tcttcccggc gcgaggatag cgcgagaccc ctcggacgtc ccgggaagag
     3661 ccagacgggg aggtgcggca ccagaagaac ctctggaagt tgagatgttg tctatcccgc
     3721 gcgccgcttt ggcgccttcg ccgatggatg ctcctccaca aactgccgta gagcgcggtt
     3781 gaccgcctcg gagtctcgga attctttcgc cacgtcggga tcgagcaaaa caaggttcgt
     3841 gcccttctga tactcagcga aaaacttccc gcggacccct ccacgcaggt cgtactccgg
     3901 ccgcatttcg tcggatgctg gcttagttgc cttcttcgta gacatagcgc tctgtagttg
     3961 ttgccagccg cgcgctgatg atccgaacgc gctggaaacg ataggtgtga gacacgatca
     4021 ccagccgctg gcgattcgaa aaaccagtgg tcagatagcg tgcctctccg gttgaatgat
     4081 caggatcctc gatggtccac tgcagcggat caccgaagac cgttgccgct tcgatgaacg
     4141 agatccgatg gaggatacgg ttacgccgct cctttgccgg atcccactcg aactgcacga
     4201 tggaagtata gctcccggct gcccatcacg atcacggctc ttgtaacctc gcgcgatcca
     4261 atccgtctca agacagtgag gagaaatcca tgatgatcac cgctgcgttg atgttcacga
     4321 tctctgccgc cacatcgacg gaaaccgcac ccctgccgcc tccgcgcctc tgcctcggtg
     4381 caccaggcag cgatctgatg gccgggcgct gcgttgatgc cgcaaagaat gagatcgacg
     4441 tcgttccgga cgggaagacc cgcggctgga tctggatcga ttccgggcgc aagacgatcg
     4501 ccttcggaac actcgcgccg gaccagtcga agatcatcat tgacgcttct ggacgctgga
     4561 aacaccggag gagcgcgtca ccttcaaccc gcgccgcctc gacgccgtta caaacgcttc
     4621 ggtgctgccc ggcgagaaca atgtgatgct cgtcgtgaag cctcccgtga cggcaggaaa
     4681 gtgagtcttg tttcacgcgg agacgcggag atcgcggagg atcggatcca tgggaaagat
     4741 tgacgaggtg ctcgcggaga tgctgcagga gcgggagcgg ctggcggccg atctcgcgcg
     4801 cgtcgaccgc gtgatcgcgg cgctggaaga gggagtcaca ctcgtgggcg acggcgaaac
     4861 gggagtggca aacgtgccgc cgggcgccgc cgcaactacc gttgcccgtg ccgctcagac
     4921 aaacttgccg cgcagaatcg gaccatatac gttgctcgac gtctacgagg cgacggctca
     4981 ttacctttca acggtcgata cgccgaagaa ctcgaccgag attgccgccg cgctccgtgt
     5041 cggcggcttc aagacacgtt caaggaactt tgtgaacacc gttcgggtga tgctgcgccg
     5101 caaagacggc agatcgaccg gcatttccgt gacgaaagac gggaagcgct ggttcctcaa
     5161 gcgaaaagcc gtgaaacgac ggccaccgaa gacctccgca gggagcgatg gggcatcatg
     5221 atcgcactgg tatgacgatg aatcacatga gtatgatcgc gcgccaaata tgtgtgatct
     5281 tcaatcaaat aagtgtgaca gaccgtcata caagtgcgac gaccggtcat acaagtactt
     5341 ccggtgatca tacgagttgg accgtgcgtc aaataagtct gaccaatggc cacacgaatg
     5401 tgaccgcaaa ccatacaagt actgccagaa gtcgctccag tatggtgcaa tccactacaa
     5461 gtatgattgc gtcaacatcg acattggcga tttgaccgtg aaccggcact tttcgacgca
     5521 atgcgccagt gaatcgcagc ccgtcgagcg acgttgcagt gcgttagccc ttcgatcagt
     5581 ttcttcttcg cgttcttcgc gttcttcgcg gcttcgcgtg atcctgctta cgtcgaaacg
     5641 ctcacgcgaa gacgcgaaga acggaggaac cgagaaccct cgaagccata cgtgcccggc
     5701 tacaggttca cccacacgct cttcgtctgc gtgtacttct ccagcgcgtc gcggccctga
     5761 tcgcgtccga aacccgactc tttgaagccg ccgaacggca gcgcggggtc gtagaggttg
     5821 tagctgttga tccagacggt cccggccttg atcgagcgag cgatcttgtg ggccttggag
     5881 atgtcgcgag tccagacggc ggcggccagg ccgtagaccg tggcgttgcc tttggcgacg
     5941 gcatcctcgg cgtctttgaa gcggatcgtg gccaggacgg ggccgaagat ctcttcgcgg
     6001 gcgatgcggt gtccgacctc gacgtcgtcg aagatcgtcg gcttgacgaa gtagcctttg
     6061 ccggtgccga tgtcggtgcg ctcgccgccg gcgacgagtt tcgcgccatc gctcttgccg
     6121 gcttcgatgt acgagagcac ggtctccatc tgttccttgg agacgagtgc gccgaggcgg
     6181 gttttggggt ggaggggatc ggcggcgacc atcttgttgg cgcgctcggt gatcttggac
     6241 atcagctcgt cgtgtgcgcc ttcttcgacg agcaggcgcg agccggcagc acagacctcg
     6301 cctttaccgt aaaagatcgc attgagcgca ccgcgcgcgg cggcttcgag atcggcgtcg
     6361 gcaaagacga tattcggtga cttgccgccg agctccagcg agactttctt cagcgtcttc
     6421 gccgcgcggg ccatcagtcc cttccccact tccgtcgagc cggtgaaggc gatggcatcg
     6481 acagcgggat ggtcgacgag cgcgttgccg acgacggagc cgcgtccggg aacgacgttg
     6541 agcacgcccg gcggcagacc gcactccttc gcgtactcgg cgaacttcag cagcgagagc
     6601 gacgtgttcg acgccggctt gatgacgacg gtgttgccgc acgccagcgc cggcgcgatc
     6661 ttccagaccg ccagcatcag cgggaagttc cagggcgtaa tggcaccaac gacaccgacc
     6721 ggctcgcgca gcgtgtaggt gaaagcgttg tctgcgatcg gaatcgtctc gcctgtgacc
     6781 ttcccgctcc agcccgcgaa gtagtagagg cactcggccg ctgcaggcgt atcgacgaac
     6841 tccgattcga agatcggctt accgttgtcg atggtctcga tgcggccgat ctcgcccgcg
     6901 cgcgcggtga gcatgtcggc gatgcgccag aggatcttgg cgcggtcgcg cggcttcatc
     6961 ttctgccagg cgctgccagg ctccatctgc gcgcgcgcag cgcgcaccgc ggcatcgacg
     7021 tcggcctccc ccgctgacgc cacgtcagcg atcttctcct cggtggccgg attggcagtg
     7081 gcgaaggtct ggcccgactg cgcgtcgacg aattcgccgt tgatgaagag cttgttctgg
     7141 atggtggagt cgtttgcgat tgcggtcata tcgcgatgct aacaaaagga gagagcgagc
     7201 gtagcgccgt cggctcgacg gctggaccgc tggcggctcg ccggcgacaa aagaaaaccg
     7261 ccaccgagcc ggtggcggta cagccgtcgg gccaacggcg ctacgcatga aggcactatg
     7321 ttcgctgcag ccggcgctac gccgtcaccg cttcttccac gtacggcgtg atgtcgaagt
     7381 tgcggacgta catctcctga atccgttcca tctcggtatc ggtcagcggc gcgtggtccg
     7441 aggcggccgc gaactcgcgg atctgttcct cgtcgtagat gttcggcagc gcggagagca
     7501 tcgacttgtg cgcgtagagc cagagcagcg cgaactggcc gagcgtcaca ccacgctcct
     7561 tccggagcga ttccagacga tcgactttct gtaagccctc gaccatccag gagcgcggcc
     7621 ggtgggagcg gtgatcggtc tcatcgaact ttgtgtcgag ggtgtacttc ccttcgagca
     7681 ttcccgaaga gtgcgtcacg cgaatgagga actggggatc gggatcggct ttccacgccc
     7741 ggaacgacgc gtcgaacgcg gatgacggcg tgcagacttt cttccccagc tctcctctca
     7801 tcgactcggg cacgcccgcg tacgacggcc ccttctcggc agccaggatg aactcgcggc
     7861 cgggatcctg ctccaggacg ttgtagatca tctgcgtcac cggggcgtgg cggacggcgt
     7921 tcgcgtagat cccttcatcg cgccagccga tggcgggacc gagcgacggg ccgtacgaga
     7981 gaatcttccc ctcgctcttg agcgactcca gcgtctccca gtgctcgtct tccatcagcg
     8041 tgactacgcg cggattgtgc ggctggtaga tggcgacgtg atcgaccccg aggcgtttca
     8101 gcgattgctc gatggcgaag cggacgaact tcggcgacat gtcctgcggc agctcacgct
     8161 gtccgcgggg gcgctcgggg ttgttgtaga agtcgtagcc gaacttcgtc ccgacgatca
     8221 cctcgggatg ttcggccagc agcggcgcaa gcatggtctc gccgcggccg ttgccgtacg
     8281 cgtcggcggt gtcgatgtag ttgattccgg cgtcgatcgc gatcgagatc agccgccgcg
     8341 cttcatcatc ggtatagaca ccccaccatc cggtggtgag cgtccaagtg ccgaagccga
     8401 gctcggagag ttggaggttg gtgccgtgga ggtttcggta gcgcatggga agggaacgat
     8461 aacagacgac gcctggactg aggtcagtgc cccgcggctc tcgacctgcc gatcgggagt
     8521 ggctgtagct ctgacccgga ataggcaacc gctcggagtg gcccgcggcc gtccgattcg
     8581 acgccggaga tcaccagacg gcctgctgcg tcggctgcca cgtcgaggtc ctcaggcccg
     8641 cggcggaatg tcgtgccgag gatcgagccg tctcgagcca ccagtgtgac catggctgtg
     8701 ccttgcacca ggcgggcgat ggcgtatgca ctgccgctcc agacgacgcc ggcgttgagg
     8761 gagttgtgga tcggataggt aacctgaagc ttcgtgccgt tgtgatggac aagggcgatt
     8821 tcagaaccgg cgacgagaaa ctccgtgccg ctgctcgcgg ccgccgaggg agcgatgaat
     8881 gcaggatcga gcggagtctc gcttccgccc tgcaacacac catcactggt caggagcatg
     8941 gcatagggcg tctgcgtcgt atccgcccca gtctggacaa agacgatgtc ctgctcatat
     9001 accgccgtga gaaggatcgc cgatccatcc cacgccagta ccggtgacag ccagaagcga
     9061 agattccgcc ccggcagcga ggggaccgat ggagcgagca cccgcagcgg tccggcgaca
     9121 ccggtcgaag tcagtgttac ggagtacatc gtgtaatccg cggaccagac gatgacgtat
     9181 ccgttcgcgg tcgcaaccgc cgcggcgccc gagaggttca caaacgaacc gaagatcggc
     9241 gtgtccccca ggattgatcc gtcgaaatcg agtcgccgcg cgatcaggcc attcgaggaa
     9301 accaggagca tgaggtaatc gagcccgttg ctgacgagcg atttccggac aatggcgatg
     9361 tctccctcga gaacagcacc gctcggccgg ccggccgcat caaccggagc ccaatgccag
     9421 cgtgagcctc gcgttccatc cgtatctttc caggccgcca ggaagtcgga accgttcgag
     9481 gcgatctgcg catccgcctg aatactggcc gtcgtggaga tgatctcggg ccccagtggc
     9541 gcgaaaagag ttctgaatac aaatgtctgc acctgccggc cggcagtcgt tggcgacgtc
     9601 gccgcgaaaa caagaccggc accttcggcg aacacgacct gctcggtttc cggcagcgag
     9661 tcgatgaccg tcatcaccgg tcccaacgcc attcccttgt tatcgagctc tgtcgcctcg
     9721 atgcgcgctg ttcccccgtc ctggacgccg gcgagaaaaa gccagagatg cgtgcccgat
     9781 tgctggacgc tggcgcgcga gagcaccagc aggggcatcg tgatcacgac gggtgcggat
     9841 acggatccgt ctggcgcgag gatcagcgtc tcgatgcgat ggtcattgct cgccacgacg
     9901 acgagaatcg atccggagtc gagctcacga accgcggcaa cgaaccagca agtcgacaat
     9961 gtgtggcaga agtcgggctt gagaagccat tgctgcagcg actgcccgtc accggagtag
    10021 cgcgtcagca tcaagtcggc tgtggtcgtg actgcgaaaa acgagttgtg gccagccgcg
    10081 atgaacgcgc gttcggccag ctcccgaatc ggccgcccct cgaagtcgac gatcgtcgag
    10141 ccgtccgagc gagagaaaac gatccggcca ggcatcgcgt acgcgcgtgt caggccggac
    10201 gggccgatcg ttctctgccc agtaaccctg ccgttgccgt cgatctgaca aagctgtgaa
    10261 ctgcggttgg cgtcatccca cacgaggacg aacccgctgc cgctcgggaa caggttcaaa
    10321 tactgcgcat tcgtcgaggg aacgattgcg aaggacggcc caagaggttt cccggctgcg
    10381 tcgctgaagg ctccccagat gccgaccgtg ggcatgaaga gctgccgcca gacggtaaga
    10441 aactttccgc ctgaaaaggc agacgacacg aaggagacgt tgcccgcggt cggtccgatc
    10501 gtcctgggct cgagctccgt ggcggagact gaaaacgcag agagagaaag gatgatgaca
    10561 gccgcaagga ggtgacggtg cattaccgcc tcgatgatag caggttgccc caaggttatc
    10621 tgtaggtaga tcagtgccct cgaacagcgc gcgctctcac ggccggactc acgacgaagc
    10681 cgtcggacat tggtgccccg ctgttcgacc gaaccgtaac catgtagtag cccacctgaa
    10741 gtgctttgaa ctgaacgact cctcgtgcac acggaacgtg gccggcgaat gccggatcgg
    10801 caatgacggc aggctgatcg atcgtgatct ggttgccgtc gattgcgaca tccggagtct
    10861 gcaggaatcc tcggaacgtc gccgtgtaga ggatcgaaac cggctggccg gcggctgggg
    10921 attggggctg aatggtgagg ccgggaacgc aggtcgacgc gtcctgcagg gtgaaggcga
    10981 atgggaaggt ctccagcgga gagcgcgaga gcgactgata cttccaagtc agcgtgtacg
    11041 aaccggaagg cagatctccg agcaagacgg attggctgtt gcacgatacc ggcggagcca
    11101 cgagcgtctc gaactccgac tgaacgacgg tgatctcgtt ccccgcaatc gtcaccgacg
    11161 gttcggacag aagagcattg acggtgtccc tgaacgtaac ccgaacgggg cctgtcacga
    11221 gggcaaaaac gggcggatcc tgaatcccgg cggaacatga tcccgtctgc gccatcgcct
    11281 gcgcggacag gaacgtaatc gttgcggtga ggaggaaaat cctggccatg tgtttgccct
    11341 caaaagcaaa aagagtcttc aggcacccgg cctgaagacc ctttgctgtg agtgcgtaat
    11401 tgtactactt ccctgcaaac tgcggcttgc gcttctgatt gtacgcgctg aggccttcct
    11461 tcgcatcttc gctctggaaa agctgctgct gcaattcgcg ctcgatggcc agggcggact
    11521 cgaagggaac ttcccagccg ctctgcacgg aacgtttgat gcggccgacg gctcgcgatg
    11581 ctttgttcgg ggggcagaag ctctgggcgt aggccatgac gcggtcccac cacttctgcc
    11641 cattctcttc ggccggccag aggtagttga tcacgccgag gcgaagagct tcctcgaact
    11701 cgaagaggcc accttcgacc atcagctcga tcgacttcgt cttgccgacg agacgcgaga
    11761 gacgctgggt gccgccggtg ccagggagga cgccgagggt gacctcgggt agtccgactt
    11821 tgccggcgcc tttgagcgcg atgcggatgt cggcagccat ggcgatctcc agtccgccgc
    11881 cgacggtgtg gccgttgagc gcggcgatga ccagcttcgg cgtctgctcg agacggttga
    11941 gcgtctcgtt cgcatggagg cagaagtagt acttgaactg cggcgtgacc tccgagagca
    12001 tcttgatgtt cgcgccggcc gaaaagaact tgtcgcccgc gccgcggagc acgatgacgt
    12061 acgcgtcgtc atccatacgc gcgttgagga tgcacgtatc gagctcctta ttcatttcat
    12121 aggtgtaggt gttggcgggg ggatcgttca gctcgaggat cgccacccct ttttccaccc
    12181 ggtaatcgat gaggtttcca gacattgaat atctcctgtt tcggtgattg aggcgggcat
    12241 tgtatgtggt ttcggggggt ggagtgtggg gtgtggggag tgggagcaga gaccgagtat
    12301 ggtggatcgg gagtcgggag tcgggggtcg gagatggagc cggactgaac ctttgcggtg
    12361 aagaagcgcg cgtcatcgca tctccgcgag acgccgttca ccagctccga ctcccgaccc
    12421 ccgaaacgcg acaccccaca aaaaacgcaa ctcccaaatc cactccccac accccactcc
    12481 ccacacccct cccatggcac ttcacttgct caacagaacg atgccggcca cgtgtgctgg
    12541 ccaactttga aggagaaaaa atgaacatcc ttggatggct tttattcggt ctcgtagtgg
    12601 gcgcgatcgc caagttcctc atgcccggac gcgacccggg cggctggatc gtgacgatcc
    12661 tgctgggtat cgccggatcg ttcgtcggag gctttctcgc ttcgatgctc ggtcacaacg
    12721 aacagacggc cggttggatc ggctccatca tcggagcgat cgtcctgctg tttatctacc
    12781 gtttaatcgt gggaaggcgt gccgtcgtct gacggccgcc ccaacccgcc ttttttgacc
    12841 tcaggccccg caaaacgggg cctttttttc gtttggcgga acgcttcaac ggtcagttgt
    12901 gttgttcgta agcgcagacg aacgggccac gcgaaacctt tcgcgatctc gttcgtctca
    12961 aggataaagg cggcagcgcc tcccgcccat tggccagtcg gggcaaatgg gcggacaatc
    13021 ccagacataa ccgatttaca cgttctttct aaggagcctc atcaacatgc acacaaccac
    13081 caaacgtatt ctgtcgacga ttgcgatcat catcgcttcg gttgccttcg gagtcgtgct
    13141 cagtgccgac ctcggcttca tgaaggcgtc gaacgcgcag tcgtcagccg ttcctgcaac
    13201 actttccaac gctccggtcc ccacggtcac gattccgtcc ttcgccgacg tcgcggcgcg
    13261 ggtcatgccc gccgtcgttt cgatccggtc gaccgaggtc atcaagacca gcgacctccg
    13321 caaaggcacc ccgttcggag gcgatccctt cgacttcttc ttccccgatc cccgcggcaa
    13381 cggcaacggc cagcggagcc ctcacggaag agtaccggga ggcgatgacg acgaacagaa
    13441 gcaggtcgcc ggaggatccg gcttcatcat ctctcccgac ggcttcatca tgacgaacaa
    13501 ccacgtcatc gacggcgcac gaaccgttga ggttcacatg ggcgctgacg agaacggcaa
    13561 cggcggccgc acgctcgaag caaagatcat cggccgcgac ccggcgaccg acatcgctct
    13621 gctcaagatc gacgccggcc aggctcttcc gtacgttccg ctcggcgact ccgaccgtat
    13681 ccgcaagggt gactgggcca tcgccgtcgg caacccctat cagttcgaaa acacgctcac
    13741 cgtcggcgtc atctccgcca aaggccgctc cctcggcctc agcaaggaga cgagctcgtt
    13801 cgagaacttc atccagaccg acgccgccat caacttcggc aactcgggcg gcccgctgat
    13861 gaacgtcaac ggcgaagtca tcggcatcaa caccgccatc cgcggtggtg gcgcccaggg
    13921 cctcggcttc gcaactccga tcaacaccgc caagcgcctg ctctcgcagc tcaagcaggg
    13981 caaagtcacg cgcggctacc tcggcatggg catcatcgac gtctccgagg atgtgaagaa
    14041 cagtttcaat ctctcggaaa cacgaggtgc gctcgtgcag acgatcgagc ccggcaaacc
    14101 agccgagcgt gcaggcctcc agcccggcga catcatcgtc gaggcagacg gccacccgat
    14161 caacaacaac cgccagctca tcgactacat ctcgtaccag ccggtcggga acaagatcaa
    14221 cctgactgtc atccgcgacg gcaagcgcaa aacgctgacc gcatcgacga tcgagcgaac
    14281 cctcgatggc gataagccgg acgtgactac ccccgatgag gccgcaccgg cccacaacaa
    14341 actcggcatg tccgttcagg agctcacgcc gcagttgcgt cagcagtacg gccaggccga
    14401 cacagtctcc ggtgtcgtcg tgacgagcgt caaggaagtc tctcccgccg gcgacgtcct
    14461 cagcgagggc gacgtcatca gcgaagtgca gggcgagaag atcagcaacc tggcgcagtt
    14521 ccgcgcagcg atcgaacacc tcaagagtgg ccagaccgcg cgtatttatg tgacctcgcc
    14581 ggccggtcgc ggcggcgccg caatctccgg ctaccgcttc atccacgtgc cataaaccac
    14641 ctcgcagcga acgaaacaca agtccccgtc accatgggtg gcggggattt tgtttttcag
    14701 cagcgcgccg gtttctttca gaccgaacac ttctcatcag ccccaactct ccaatcgagc
    14761 gccttctccc catcgcttct cagatgggga gaaggtggcg aagccggatg aggggccaca
    14821 ggatgacatt ctctccacgt acaatcgcgc tcgtggaaga cctcatgcga cgtgcgctcg
    14881 atctcgctgc gcgcggccgc tactccgtga gtccgaatcc gatggtcgga tgcgtcatcg
    14941 tccgcgacgg gaacgtcatc ggcgaaggat ggcatcaccg cgccggcgag ccgcacgccg
    15001 agatcgaggc gctgcgcggt tgcgacgcgc gcggcgccac gatgatcgtc acgctggaac
    15061 catgcgcgca tcacggccgc acgccaccct gcgtcgacgc catcgtcgac gccggcgtcg
    15121 cgcgtgtcgt cgtcgccatg cgcgatcccc acgatgcggc cggaggcggc atcgagaagc
    15181 tgcgcgaagc aggaatcgag gtcactaccg gcgtgctcga acccgaggcg cgccgcctca
    15241 acgagacgtt catccacgct gtcacgcacc ggcgtccgtt cgtgctgctg aaagcgggta
    15301 tgacgctcga tggcaagctg gccaccgtga ccggcgaaag ccagtggatc acctcggaag
    15361 catcgcgcga gaggagtctc gccctgcgcg aggagtacga cgcgatcctc gttggcggcg
    15421 gcaccgtccg cgcggacaac ccgcgcctca cacgccgcct cggcttgaac acatctgctg
    15481 caggctggac gcgggtcatc ctcgatcgca gctccgttgt ttcgcttgat gcaaccgttc
    15541 taaccgacgg cggaccgacg attcacatca cccgagacgc cgatctcgac gagctcctgg
    15601 caggtctcta cggacaaggc atccactcgg tgatcgttga aggaggttcg acagtgctct
    15661 cggagttcat ccgtcgaggg atgtggcaga agatgatcct cttcgtcgca ccgatggtcg
    15721 tcgaaggagc gacggcccct tcgattttcc gcggcgaggg catcgcgaag ttgacagatg
    15781 ctccacgctt tcgctttgat cgcgtcgaga ctgtgggaga ggatctgatg attactttgt
    15841 attcgggggg tggggagttg ggagtgggga gtgggagtga accagcaact ccagtccgat
    15901 caacggcaca tccatcaact ccgaacacgt catctcccac cccccacccc ccaaaaccca
    15961 ccccctaatg ttcaccggca tcattcaccg caccggcatc atcgaatcgc tcgtcacaaa
    16021 cgagtcgggc gcgcaactgc gtctgcgcac cgaggatgcg gagcccttca cccgcggcga
    16081 gagcctggcc gtcaatggtg tctgcctgac tgttcttccg caggatgacg gatcgctcct
    16141 caccgatgtc tcgaacgaaa cgctcgcacg cacgacgctc ggcgctctgg ttcgcggcgc
    16201 gcgcgtgaat gtcgagcgtg cgatggccct cggcgaccgg cttggcgggc atctcgtgca
    16261 gggacacgtc gacggagtgg gaacgctgca gtcgatcaag cgcgagggcg cattcgcggt
    16321 ctatcgctgg agccatcctg ccgagtatgc ggcgctcatc gtcgacaaag gctccatcgc
    16381 cgtcgatggc gtgtcgctga cagtcgtgga acccgatggc gccacattcg gcgcggctct
    16441 catccccgag accctttcgc gcacgaacct cggcgacgcg gccgaaggcg gccgggtgaa
    16501 tctcgagctc gacatgctcg ccaagtacgt gcaatcgctg gtcacgccat acctccggcg
    16561 ctgagacgcg cattacactg tcgcggcttg atcgtctaca agaagacgca gcatctgaag
    16621 ctcacccgcg cgcatgtggc cgagccgccg tattgggcgg ccacgacgat tgcgccctac
    16681 agtccgcggc gcgccgcccc gatcgccatc gactacatcg atctgcgcgc gtcgtgcgcg
    16741 gaccgtttcg aggtggcggt gtgcgagaac gtgcgcgacg acctcgagcg aaccaccacg
    16801 cgtcatccgc tgcatgcgcc ggtgatgatc gatgctaccg agttcgccga ggtgatcttc
    16861 aggcgcggcc aggaagcgct gcagttttgc gccgagcagc aggtggcggc gttgcacctc
    16921 gtttccacgc gcggcgagct gccggcagaa gttcaggatg atgccgtcat cgctgtcgcg
    16981 ccctggccac tcgagtttcc gcgaatcgaa atgctgttcg ccgaggcaaa ggaacgggga
    17041 ctgcggtggg gcgtcgtggt tccgatcatt tatccggtca cgaccgatct caccgcgttg
    17101 gccgagctcg ccgaattagc aaagaagtat gccccttcgt tcttcgccgg agtgccggtc
    17161 gagctcgacg ccacggcgcg gaacggcctt gcgcgatctc tcaccgggga cgaccagaac
    17221 gacacttacg agatgctgtt ccacgcggac ctcgggccga ttcgcctggc gaccgagcgc
    17281 cacatcgcgg cgctggcggg cgagatcaac gccgaggact tcgtcattcc gccgcgatgg
    17341 gaccgccaca ccaactggaa cggtgcggtg ctgctgacgc tcctcgccaa cagaatcctg
    17401 gcgatgaatc gcgacgaaga cctggcctgg cgcctgaccc gatcggccaa agtcatcgca
    17461 caactcgaga agccgatcga gcgggtggcg caggcggcca gcctcggcat catcgactcg
    17521 ctcgacgaga tcagcatcga cctcctgacc gagtggctcg aaaccggcag atcaagcttt
    17581 gccggacaca tcgagaagca gtggcgcctg cgaagagaca gcgggatgta gagtttgtct
    17641 ctgattctga ttccgaaagg ccgaaggccg gaagggccgc cgtacccttg aattcttcct
    17701 tggatcctac cgggagagcg gaaggatagc agaaaattcc aaaagcaaac gaaattctgg
    17761 aatccatgac gcgacgacga cagattttga cccttctcgg cgctacggcg ttcatcgtgg
    17821 ccatcgcggc ggcgaccgtc tggtttctcg ttcgccggat cgatcaggcc gtcgtcagcg
    17881 ccgtgacttc gaaggaggaa acggtcgatc tgggaacgct cgtgacgcgc gtccgcgacc
    17941 tcaaccgcct agagaccgca accatgcgcg tcaccagcat cagcacggtc aagcagacct
    18001 acgacctgat tccgaacgcg cttgccggcg acgagctgac gctctatgcc acgggcgacg
    18061 tcatcgccgg cgtcgacctg tcgcagttga agcaggagga tgtccgccgc gagcctgacg
    18121 gcacgatcgt cgtacgcctc ccgccgcccc aactcctcgt ctcgcgcatc gacaaccgca
    18181 cgacgcgcgt caccggccgc cgcacgggcg tcttccgccg cgccgacatc ggcatggaag
    18241 gccgcgcacg ccaatacgcc gaacagaaca tccgcaacga agcggtccac aaaggcatcc
    18301 tccccctcgc gcaacagaac gccgaggcga ggatcgcggg tctgctgcac acgctcgggg
    18361 cgacgaaggt gcggttcgtg gggatagagg gaaacgctgg ggcgaggtag cgacagtcga
    18421 tggagacggt ttcacgcgga gacgcggaga ccgcggagct gagattgaaa cgacttcgcg
    18481 tcttcgcgtc ttcgcgtgaa ccgtctcctg ctcttcaact cgccacaacg atgctgcagc
    18541 acgtcagcgc gccttctgct ttcgcaagct ccgagacatc gaccgtccgc acgttgacgc
    18601 cggcgtcacg aagacgctgc gcggttcggg gaaaggcggc cgcacagagc acgacgtcgc
    18661 caacgcggag gacattcgcg gccgacggtt cggagggatc gacggcgatg acctgcattc
    18721 catcgaacag cgatgcatcg acccactccg ggttgaggac cacgccatcg tcagagaacg
    18781 cggtcacggc cgatttgaga tgaaggcatc cccggggggt gacggctcgg acggaatagc
    18841 cgaacgggat gagtgcggct gaaagttgcc ggaagccttc cgcgtttgtt ctggtggact
    18901 ggccgacgaa gatctttcgg ccgatgcaca gcacgtcgcc gccgtcgaga atcgccggtg
    18961 gctcgatgaa aaccagctcg cggtagttgc gcagcgcggc ggcggttgat gtcgtttcgg
    19021 gccttcgcga ctctgcgccc ggccgtgtga tgatcgcgat ctcatcgagc acgatcgccg
    19081 tgtcttcgac gaagacggag tccggtagct cgtcggcggc aggcagtcgc tcgattctgc
    19141 agccggcgtc ggttagcgcg gcctcgtacg cctgatgctg tttcctcgcg atatccacgt
    19201 cgatcgggtt gcgatcgatg tgcgttagtt cgcagcgggt gatcgcctgc gggacggcac
    19261 gggtgagcgc gacgagcatg gttcgcgcat tgtatcgagc acactccaca agccgcgaag
    19321 agtcgccgcc gggccggcgg cggaacagcc ggccagcggc cggcgctacg tttggatggc
    19381 gctatgttgg cttgttcaag acggtcgtcg cgattctgat cgctagcttt agtgcggcgg
    19441 acttgctcat cgtctcctcg tactcgcgcg cgggatcgga gtcgtagacg atgccggcgc
    19501 cggcttgcac cgacgccttg ccgttggcga ggacgattgt gcggatggtg atgcaactgt
    19561 ccatgttgcc gctgaatccg aagtaggcga cggcgccgcc gtacggaccg cggcgtgtgg
    19621 tctcgtgctc gtcgatgagc tgcatggcgc ggatcttcgg cgcgccggtg agcgtgccag
    19681 ccgggaagca ggagaggaag agatcgaccg gactgcggtc gtcgcgcagc gttccttcga
    19741 cgttcgtgac gaggtgcatg acgtggctgt agcgctcgat gtggcagaag tcggagacgg
    19801 acaccgatcc gctcacgcag acgcgcccga ggtcgttgcg gccgaggtcg acgagcatga
    19861 ggtgctcggc ctgctctttc ggatcggcca cgagcgcgcg ctccaactct gcatcctcct
    19921 cctcggtcgc accgcgcggg cgcgtgccgg cgatcgggcg cgtctccgcg cgcgtgccgt
    19981 cgacccggac gagcatctcc ggagacgccc cgacgagcgt gcactccggc gtgcgcaaca
    20041 ggaacatgta cggcgatggg ttgaccgagc gcagcacgcg atagagcgtc agcgcctccg
    20101 attccgggaa tggagtgctc cagctctgcg acaggacgat ttgaaagact tcgccggctg
    20161 tgatctcttc cttcgctgcg cgcactacct cttcgaaatc gggacgcggg atgttcggcg
    20221 tgaatgcaac gtcgacgacg tgctcgggga acgcgagcag ctccacacgc gcggcgcgca
    20281 accgctcgat ggcgctgtcg aggcgggcat tcgcttcctc gaacgacgca tgcgcatcgg
    20341 acgtgaagag actggcgatc acgtacaggc gttgcttcac gtgatcgaag acgacgacgt
    20401 tatcgaagaa gatcagccgc gcgtcgggga tgccggtctc gttttgatac gaatcgggga
    20461 tgcgctcgat ccagccggcc acgctgtagc cgaagaaacc gacggcgccg ccgaagaacg
    20521 gcggaagcga ttcctcgccg taaacgcgca gcggctgcag ctcgttgttc aacacgccga
    20581 ccggatcggg cgtcgactcg tcgaagcgaa ggctgcgccg cggctcaaag ccgacgaacg
    20641 agtagcggcc gacgttttcg ccgcgttcga cgctctcgaa cagaaaggcc tcgccgtcgc
    20701 cggatagggc ggcgaatgcc gcaacagggg tgatggcgtc cgcgctcaac tcgcgagcga
    20761 tgggaacgat gt
//
LOCUS       DATLVW010000387         7381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_350593, whole genome shotgun sequence.
ACCESSION   DATLVW010000387 DATLVW010000000
VERSION     DATLVW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350593"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VNN25_11665"
     CDS             complement(<1..420)
                     /locus_tag="VNN25_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase, group 1"
                     /protein_id="HXH92230.1"
                     /translation="MTTVCYILSHYISHRQAGLQFQDCFRRLGVRLVETPAEADLVII
                     HNEPWSVAGYYRVFPELREKRVVTYAVWETDQLPESYRFNLSLSSEIWTCSGYSRDAL
                     AQSGRPVSVVPHVVSVPEPNAEAERRMRERIGIRDGEM"
     gene            complement(428..2128)
                     /locus_tag="VNN25_11670"
     CDS             complement(428..2128)
                     /locus_tag="VNN25_11670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH92231.1"
                     /translation="MPVLDQLRGTSSCAIYYASEAYDGEQPGVVGRQSAGAGFLEALV
                     RYGSQDELFCLTGQQTQFNEFQRKVSTLMADPPPSTWIRPDDLETLGRAGCVYNPGPI
                     IAEMAWLRRYGDERLFSICGITHSVATDRAIRGIRDFLTAPTQPWDALVCTSQSAQAA
                     VNRILESWTDYLSRRGFHVPESPMRSCIIPLGVNIDRFRRDASAIDRGRALRAKLGIG
                     EDDVAGLYFGRLNFLSKAHPTPMYRAFELAQRTMPPGRLHLIMTGQFNDPRMAPQFEA
                     ARQRFCPSVRVHLVNGADADAADSWFAADFFISLTDNIQESFGLTPVEAMAASLPCII
                     SDWDGYRETVVDSESGFLISTTTPPPGAGVELANQLARRAFDHFSYIGLVSQSTAVDI
                     DQCARAIMTLAGNAGLRQRMGEAARKRAENVYDWRGIIRQYLALWSDLGALRASAPAT
                     GLREPNATVHPDYPDPFSMFRAHPTRVLGSEDVVRLADLDGRFVLAQLRKNAMHTLAT
                     PSLLESAAIDALIAAVESGPKSIAELGGLSGAGDYARMLRTIAWLYKYGIVSLSPSVD
                     "
     gene            complement(2138..4087)
                     /locus_tag="VNN25_11675"
     CDS             complement(2138..4087)
                     /locus_tag="VNN25_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH92232.1"
                     /translation="MVAIVHHHILPRVSTGVNDWIPDYPNPADFQFDYYKLLALAASN
                     DGGIGSAPGPGINVAVVGAGAAGMTAARELFRAGYNVTIFEASNRLCGRHWTVQGSAA
                     TGMELGAMRFPFFPAPGANNSVFEYYLRTEADAIIFPFPNPGSAPNDTGIYLSQGYGP
                     NDQFYPTPTMISWLYGQEAPDNPALAAVSTLVNNFINFFTGVVGGTNDNPGLYASPDW
                     PVYWAQIANNYDKMSFSDLVFSPAINSYNNDGWLGGFGMNDAQSQLFYTIGAGDGSWG
                     AFYEIAAMWFIRCVMFGFNSDLQTLTGLNGASSLPYYNDWFLNDSAGNRLPAPLYSGI
                     QSLVEWLFYNPAPGATQSLFNAVNDGGGASLFINRAVQSITWNDSNLIVHDQSGSDTA
                     FDYVVVTPEIWASQLGVIFKGFSPNDMPSTTLAARDQQHIIASCKVFFPLTEPYWEDP
                     NCAIPQDIVTDTFVQDAYGIKWQPNDTGVLLASYTWEDDALKLLPYNDAQVAQMVLAE
                     LDRITTTTLGNDQTVSQYVTDLNDAVVFQWSQQVGYNGCAKLYRQRDWTQSYSLLAYN
                     QQLSHSSRLYLAGETFGVEGGWTEPALRMALDAVIRIIQNSGGSFVPGFDVANDYPRF
                     DVIFTPNYNYNVSVNPPPKPKRTTS"
     gene            complement(4300..4827)
                     /locus_tag="VNN25_11680"
     CDS             complement(4300..4827)
                     /locus_tag="VNN25_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXH92233.1"
                     /translation="MRVVAIVLAAGSSTRLGRPKQSIVLDGETLLQRAERVARAVADE
                     VIVVTEELNPDAAEGIASSIRTGVRLAGDDARILITLCDQPLITAEHLRALLGVDAPI
                     VATGYAGTAGAPAVFAPSLIRELLALRGDRGARAVIEAHRDIAKVVPFEDAAVDIDRE
                     DDVRELLSRVSAGKQ"
     gene            complement(4818..5690)
                     /locus_tag="VNN25_11685"
     CDS             complement(4818..5690)
                     /locus_tag="VNN25_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXH92234.1"
                     /translation="MHEIDKIIAVCRSVIEAGSRGVLVTVVRTQGSTYRRAGARAVIG
                     EDGTLAGAISGGCLERDLGERLSVWLADMSPRLITYDSTRSDDLIFGLGLGCRGMLDL
                     LIEPFDAQHPPRLVTDFHWNGLEPAEWTTILPNGESMIEILRPPRAVVIFGGSDAEPV
                     VQLTRAIGWRVEVIKPRAPFDPRGFDAAVVMTHNFGRDAEILRQLLASPIPYIGLLGP
                     KSRGEELLAEIGASKDARLHSPIGLDLGAETPEEIALSIVAEIQAVFERRSARSLREL
                     ATPIHETVDKPACA"
     gene            complement(5716..6903)
                     /locus_tag="VNN25_11690"
     CDS             complement(5716..6903)
                     /locus_tag="VNN25_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92235.1"
                     /translation="MTSSIKITRSDFTRYAGVGVVALILACSGHTPRPVSGGSDSGYK
                     RGTEEGMEGFTEGDKRVHAGSRFSAVRDAVFANPYQKVWGAKGNAPLERFPVTLGPLI
                     KGMLPGGGTWQFLAAARRVLASDSDLRWGPDGKGYQRLLHTNGIIMTGRWEITDDTPY
                     SGYFHNGSRGLIIARYSTCCTETRRGNMRSLSMIGRIYPTTDPNHPEPLPTACFITQE
                     DIGGEVTANINDARLRNAPNTTVTRRGSGMPILILSGIVFKKAEKQPTIRQVYQIAEL
                     GKPPAEPTRAPEFMQLTVAGGQPVIAGDNLDFRDEIMAHIYDPGDPTPKRKLVFNIET
                     SDTGTTHGTAFKERRDITNWKKIGTITFDAAVVSFNGDHVFHVNHPAWREDRNDPRTV
                     VQK"
     gene            complement(6916..>7381)
                     /locus_tag="VNN25_11695"
     CDS             complement(6916..>7381)
                     /locus_tag="VNN25_11695"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH92236.1"
                     /translation="HRSFLSYVGIVADVSLNDRNEVTINDIYGVIDCGLAVNQDRVMA
                     QMEGGIIYGLSYALLGEITVKKGAVVQNNFDDYPILRIHQAPKIHAYVQDPPAEVVKA
                     YDGNVPPTGVGEPPTPAVAPAVANAIFAAGGPRIRAIPFGKKRENGEWVVVL"
BASE COUNT         1376 a         2202 c         2407 g         1396 t
ORIGIN      
        1 catctcgccg tcgcggatac cgatgcgctc gcgcattctc cgttcggcct ccgcatttgg
       61 ctccgggacg ctaacgacgt gagggacgac cgacaccggg cggcccgact gagccagcgc
      121 atcccggcta tagcccgagc aggtccagat ctcggaggaa agcgagagat tgaagcgata
      181 ggactccggc aactggtcgg tttcccatac tgcgtaggtg acgacgcgct tttccctgag
      241 ctcggggaag acccggtagt acccggccac gctccacggc tcgttatgaa tgatgaccag
      301 atccgcttcg gctggtgttt cgacgagccg tacgccgagc cggcggaagc agtcctggaa
      361 ctgcaggcct gcctgccggt ggctgatgta gtgactgagg atgtagcaaa ccgtcgtcat
      421 ggcggcctca atcgacggac ggactcaggg agacgatgcc gtacttgtat agccaggcga
      481 tcgtacggag catccgggcg taatcgccgg ctccactcag tccgccgagc tccgcgatgc
      541 tctttggtcc gctctccacc gccgctatga gcgcgtcaat cgcggcgctc tccagcagac
      601 tgggcgtcgc caatgtatgc atcgcgttct tgcgcaactg agcgaggacg aatcgtccgt
      661 cgagatcggc gagccgcacg acatcctcgg atccgagaac gcgagtcgga tgcgcgcgaa
      721 acatcgagaa gggatcggga tagtcggggt ggacggtcgc gttcggctcg cgcagtcctg
      781 tcgccggcgc cgaagcgcgc aatgcaccga gatcgctcca gagcgccaga tactgcctga
      841 tgatcccgcg ccagtcataa acattctcgg cgcgctttcg agccgcttcg cccatccgct
      901 gccgcaggcc ggcgttcccc gccagcgtca tgatggcccg cgcacattga tcgatgtcga
      961 ccgcggtgct ctggctcacc aggccgatgt agctgaagtg atcgaacgcc cggcgggcta
     1021 actggttggc cagttcgacc ccggcaccgg gcggaggcgt cgtcgtggag atcaggaacc
     1081 ccgactcgct gtcgacgacg gtctcacgat agccgtccca gtcgctgatg atgcagggga
     1141 gggaggcggc catcgcttcg acgggcgtga ggccgaagct ctcctgaatg ttgtccgtga
     1201 gcgagatgaa gaagtccgcg gcgaaccagg agtcggccgc gtcggcatcg gctccattga
     1261 cgagatgtac gcgtaccgag gggcagaagc gctggcgcgc agcctcgaac tgaggtgcca
     1321 tgcgcgggtc gttgaactgc ccggtcatga tgaggtgcaa ccgccctggc ggcatcgttc
     1381 gctgcgccag ctcaaaggcg cggtacatcg gcgtcgggtg cgccttggag aggaagttga
     1441 gccgtccgaa atagagcccg gcgacgtcgt cttctccgat gccaagcttc gcgcgcaacg
     1501 cacggccgcg atcgattgcc gatgcgtcac gccggaagcg gtcgatgttg acgccgagcg
     1561 ggatgatgca cgagcgcatc ggactctcgg gaacgtgaaa gccgcggcgc gagaggtaat
     1621 cggtccacga ctccagaatg cggtttacgg ccgcttgcgc cgattgcgac gtacacacca
     1681 acgcatccca cggctgcgtg ggagcggtca ggaaatcgcg gatgccccgt atggcgcggt
     1741 cggtggcgac ggaatgcgtg atgccgcaga tgctgaagag ccgttcgtcg ccgtaacggc
     1801 gcaaccatgc catctcggcg atgatcggac ctgggttata gacgcatccg gcccgtccga
     1861 gcgtttccag atcatcgggc cggatccagg tgctcggcgg cgggtccgcc atgagcgtgc
     1921 tcactttgcg ttgaaactcg ttgaactggg tctgctggcc cgtcaggcag aagagctcat
     1981 cctgtgagcc gtagcggacg agagcctcga gaaagccggc tccggcggac tgtcttccga
     2041 cgacacccgg ttgctcgccg tcatacgcct ctgaggcata gtagatggcg caggagctgg
     2101 tgccgcgcag ctgatcgagc accggcattg gtcgcaatca ggaagtcgtg cgtttcggct
     2161 tgggtggcgg attgaccgag acgttgtaat tgtagttggg cgtgaagatc acatcgaacc
     2221 tcggatagtc attggcgaca tcgaagcccg gcacgaacga gccgccggag ttctgaatga
     2281 tgcggattac ggcatcgagc gccatgcgca acgccggctc cgtccagcct ccttctacgc
     2341 cgaacgtctc cccggccaga tagagcctcg agctgtgact gagttgctgg ttataggcga
     2401 gaagcgaata gctctgcgtc caatcgcgct gacgatagag cttcgcgcag ccgttgtagc
     2461 ccacctgttg agaccactga aacacgacgg cgtcattgag gtctgtgacg tactggctga
     2521 ccgtttgatc gttccccagc gtagtggtcg taatcctgtc cagctcagcc aacaccattt
     2581 gtgcgacctg cgcatcgttg tacgggagga gcttgagagc gtcgtcctcc caggtgtaac
     2641 tggccagcag taccccggtg tcgttgggct gccacttgat cccataggca tcctggacga
     2701 acgtgtccgt gacgatgtct tgcgggatcg cacagttggg atcctcccaa tacggttccg
     2761 taagggggaa gaagactttg caactggcaa tgatgtgctg ttggtcgcgc gcggcgagcg
     2821 tcgtcgacgg catgtcgttc ggcgagaatc ctttgaagat gaccccgagc tgcgaggccc
     2881 agatctccgg cgtcacgacg acatagtcga atgccgtgtc gctccccgat tggtcatgca
     2941 cgatgagatt cgagtcattc caggtgatcg actgaactgc gcggttgatg aagagggaag
     3001 cgccgccgcc gtcattgacc gcattgaaga gcgactgggt tgcgccaggc gccggattgt
     3061 agaacagcca ttcgaccaga ctctggatgc cggagtaaag cggagccggg aggcggttgc
     3121 cggctgaatc gttgaggaac cagtcgttgt aatagggaag gctcgacgca ccattcaggc
     3181 cggtaagggt ctggagatcg ctgttgaagc cgaacatgac acagcggatg aaccacatcg
     3241 cggcgatctc gtagaacgct ccccaacttc cgtcgccggc gccgatcgta tagaagagct
     3301 gcgactgcgc gtcgttcata ccgaacccgc cgagccaccc gtcgttgttg tagctgttga
     3361 tcgcgggcga aaagacgaga tcggagaacg acatcttgtc gtagttgttg gcaatctgcg
     3421 cccagtaaac cggccagtcc gggctcgcgt aaaggcccgg gttgtcgttc gtgccgccga
     3481 cgactccggt gaagaagttg atgaagttgt tgacgagggt ggagacggcc gccagggcag
     3541 ggttgtccgg cgcttcctgg ccgtacagcc aggagatcat cgttggcgtc ggatagaact
     3601 gatcgttcgg tccatagccc tggctaagat agattccggt gtcgttcggc gcggagccgg
     3661 gattggggaa aggaaagatg atcgcgtcgg cctcggtgcg caagtagtac tcgaacaccg
     3721 agttgttggc tccgggcgca ggaaagaacg ggaagcgcat ggcgcccagt tccattccgg
     3781 tggccgcact tccctgaacg gtccaatgac gtccgcagag gcgattgctg gcttcaaaga
     3841 tcgtgacgtt gtagcccgca cggaacagtt cacgggccgc ggtcatgccg gccgctcccg
     3901 ctccgacgac cgccacgttg atgcccggcc caggggcact gccgatgcca ccatcattcg
     3961 acgcggccaa tgcgagcagc ttgtagtagt cgaactggaa atccgcaggg ttcgggtagt
     4021 ccgggatcca gtcgttcacg ccggtcgaaa ctcgtggaag gatatggtgg tggacgattg
     4081 caaccattgc ttctctccct tcctcggacc cttcggaggc aggaatcagt cagttaagaa
     4141 cgcggaagct attccggaaa tgtacacctg actgttggca aaaacaatgg ccatccgtca
     4201 gttatcggtg tagtcgccgg aatcacaaaa accgtccacc acagaggctc agaattcacc
     4261 gagccgcagt caactcggtg ccctcagtgt ctctgtggtt cactgttttc cggcggagac
     4321 gcgagagagc agctcgcgga cgtcgtcctc tcgatcgatg tcgactgctg cgtcttcgaa
     4381 gggaaccact ttcgcgatgt cgcgatgagc ctcgatgacg gcgcgcgcac cgcggtcgcc
     4441 gcgaagggcg agcaactccc gtatcagcga tggcgcaaag actgcgggtg ctccggcggt
     4501 gccggcataa ccggtggcca cgatgggagc gtcgacgcca agaagagcgc gaagatgttc
     4561 ggccgtgatc agcggctgat cgcagagcgt gatgagtatc cgcgcatcat cgccggccag
     4621 ccgcactccc gtccggatcg acgatgcgat cccctcggct gcatcgggat tgagctcctc
     4681 ggtgacgacg atgacttcgt ccgccaccgc acgcgccacg cgctcggcgc gctgcaacag
     4741 cgtttcgcca tcgagcacga tcgactgctt cggccgtccc aggcgcgtcg acgatccggc
     4801 agcgaggacg atcgcgacta cgcgcacgcc ggtttgtcca cggtctcgtg aatcggcgtc
     4861 gcgagctcgc gtaacgagcg cgcggaacgg cgctcgaaga cagcctgaat ctcggccacg
     4921 atcgatagcg cgatctcctc gggtgtctcg gcgccgaggt cgaggccgat cggactgtgc
     4981 agtcgggcgt ccttcgatgc tccgatctcc gcgagcaact cctcgccgcg cgacttcgga
     5041 ccgagaaggc cgatgtacgg gatcggcgac gccaggagct ggcgcaggat ctcggcgtcg
     5101 cgcccgaagt tgtgcgtcat cacgacggcg gcatcgaagc cgcgcggatc gaacggtgct
     5161 cgcggcttga tcacttcgac gcgccatcca atcgcacgag tcagttgcac gaccggctcc
     5221 gcatccgagc ctccgaagat gaccactgcg cgcggtggac gaaggatctc gatcatcgac
     5281 tcgccgttgg ggaggatggt cgtccactcc gccggttcca gcccattcca atgaaagtcg
     5341 gtcacgagcc gcggcggatg ctgtgcgtcg aacggctcga tgagaaggtc gagcatgccg
     5401 cggcagccga gacctaggcc gaagatgaga tcgtcgctgc gcgtggaatc gtaggtgatg
     5461 agccgcggag acatgtccgc aagccagacg gagaggcgtt cgccgaggtc gcgctcgaga
     5521 caaccgccgc tgatggcacc ggcaagagtt ccgtcttcac cgatcacggc gcgcgcgcca
     5581 gcacgccgat acgtcgatcc ctgcgtccgg acgacagtga ccagcactcc gcggctgccg
     5641 gcctcgatca ccgaacggca gacggcgatg attttgtcga tctcgtgcat ggctgaggag
     5701 aggataaggg ctttgctact tctgcaccac cgtccgcggg tcgttccgat cctcccgcca
     5761 tgccggatgg ttcacatgaa acacgtgatc tccattgaac gagaccaccg cggcgtcgaa
     5821 ggtgatggtt cctatcttct tccagttggt gatgtcgcgg cgttccttga atgcagtgcc
     5881 gtgtgtggtg ccggtgtcgg aggtctcgat gttgaagacg agcttgcgtt tcggcgtggg
     5941 gtcacccgga tcgtagatgt gggccatgat ctcgtcgcgg aagtcgaggt tgtcgccggc
     6001 gatgaccggc tgccccccgg caaccgtgag ctgcatgaac tcgggggcgc gcgtcggctc
     6061 ggcagggggt ttcccgagct cggcgatctg gtagacctgc cggatggttg gctgtttctc
     6121 ggcctttttg aacacaatcc cggacaggat aaggatgggc atgccgctgc cgcggcgggt
     6181 gacggtagtg ttcggcgcgt tgcgcagcct ggcgtcattg atgttcgccg tgacttcgcc
     6241 gccgatatct tcctgggtaa tgaagcatgc ggtcggcagc ggctcaggat ggttcggatc
     6301 ggtggtgggg tagatgcggc cgatcatcga cagagagcgc atgttgccgc gtcgcgtttc
     6361 ggtgcagcac gtggaatagc gggcgatgat caggccgcgg ctcccgttat ggaagtagcc
     6421 gctatagggc gtatcgtcgg taatttccca ccgcccggtc atgatgatgc cgttcgtgtg
     6481 gaggaggcgt tggtaacctt tcccgtccgg gccccagcga aggtcagaat cggaagcgag
     6541 gacacgacgc gctgccgcga ggaactgcca cgttccgccg ccgggcagca tgcctttgat
     6601 caacggcccg agcgtgaccg gaaagcgctc gagcggcgcg ttccctttcg ctccccatac
     6661 cttttgatac gggttggcga aaacggcgtc gcgcacggcg ctgaagcgcg agccggcgtg
     6721 cacgcgctta tcgccctcag tgaagccttc catgccctcc tccgtccccc gcttgtatcc
     6781 ggagtcagaa ccgccagata ccgggcgcgg cgtgtgaccg gaacaagcga gaatgagggc
     6841 gacgacgccc acaccggcgt aacgggtgaa atcgctgcgc gtgatcttga tagagctggt
     6901 catgaagcct caggcttaga gcaccactac ccattcgccg ttttctctct ttttcccaaa
     6961 tggaattgcg cggatgcgag gtccgccggc tgcgaagatc gcattggcta cagcgggagc
     7021 caccgcgggc gtcggcggtt cgcccacgcc ggtcggcggt acgttgccgt catacgcctt
     7081 caccacttcc gcgggaggat cctggacgta cgcgtgaatc ttcggcgcct gatggatacg
     7141 caggatcgga tagtcgtcga aattgttctg caccacagcg ccttttttca cggtgatttc
     7201 gccgaggagg gcatagctca ggccgtagat gatgcctccc tccatctggg ccatgacgcg
     7261 gtcctggttg acggccaggc cgcagtcgat cacgccgtaa atgtcgttga tcgtgacctc
     7321 gtttcggtcg ttgagcgaaa cgtcggcgac gatacctaca tagctcagaa agctgcgatg
     7381 g
//
LOCUS       DATLVW010000388         7897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_265865, whole genome shotgun sequence.
ACCESSION   DATLVW010000388 DATLVW010000000
VERSION     DATLVW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265865"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VNN25_11700"
     CDS             <1..516
                     /locus_tag="VNN25_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH92237.1"
                     /translation="GGLRIDTKYPKSNDGILEWIAGTSVNLSVTYEVVVPRTMSLQIE
                     NTNGGVEASDVSGSHRVETTNGHIRLARCAGDLDAETTNGGIEAELTSVTAGHVVRLE
                     TTNGAIRARLPRKLAASLDASTTNGGIESDLPVATRGKLEKHTLRGTINGGGPDLRLR
                     TTNGSIHIQGI"
     gene            complement(856..1383)
                     /locus_tag="VNN25_11705"
     CDS             complement(856..1383)
                     /locus_tag="VNN25_11705"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HXH92238.1"
                     /translation="MDETPFSGAAEDEDLAREIADNAEAESLPRDRASRFYDRIRKTT
                     HSYLDRKSRVAGKAGEFLLLVPDVFILLWRLTTDSRVNSKDKVLLGSAVAYYIMPFDL
                     IPEAIVGPIGYLDDLVFGVMVLSKVLSSVDAAVVQEHWSGSEDVLVAIKRVLNAAEGL
                     IGSDLVSKIKKMIMR"
     gene            complement(1376..1849)
                     /locus_tag="VNN25_11710"
     CDS             complement(1376..1849)
                     /locus_tag="VNN25_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92239.1"
                     /translation="MQRFITWDQLEVRVDGEKINAMAAGFRVDPIERMELQFFNGLLR
                     VSGTIRKFISVPFTVDIKEIRASGRTVRVPIASASAFGALPIPQFLFGLAKNKLPADL
                     VHYEEPATFVVSLERFLPSFVDADIENIWIIDGGLAVTLGRGGADLPPSSEKANG"
     gene            2021..5215
                     /gene="carB"
                     /locus_tag="VNN25_11715"
     CDS             2021..5215
                     /gene="carB"
                     /locus_tag="VNN25_11715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXH92240.1"
                     /translation="MPKRTDIHSILIIGSGPIVIGQACEFDYSGTQACKALKDEGYRI
                     ILVNSNPATIMTDPEFADRTYIEPLTPETLERIIEREKPDAILPTVGGQTGINLAMAL
                     HENGALERHGVELIGANSLAIRTAEDRQLFKDKMVEIGVEVPRSVHAATEADAEEFAA
                     TIGYPLVIRPSFTLGGSGGGIAYNVEELRAVVSHGLELSPIHRVLVEESVTGWKEFEL
                     EVMRDKADNVIIVCSIENLDPMGVHTGDSITVAPQQTLTNTEYQAMRDEAKRIIRAIG
                     VETGGSNIQFAVHPTTGRRLAIEINPRVSRSSALASKATGFPIAKFAAKLAAGYTLDE
                     IPNDITKKTPASFEPAIDYVVVKIPRFAFEKFRKTKPNLGLSMQSVGESMAIGRTFNE
                     ALQKGLRSLEIGVSGLESRGYDRDKAASLLRTPQPERIFAIGDAFRAGMTVDEVNAIS
                     SIDCWFLREIYKIVEMEKSITGNPTAKSLLAWKREGFSDKQIASLTNLTEGEVRGQRY
                     ALGVRPVYKRVDTCAAEFEASTPYLYSTYEKENEANPTSRRKVMILGSGPNRIGQGIE
                     FDTCCVHASFALREIGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIVDLEKP
                     EGVIVQFGGQTPLKLAKSLAEAGVPMWGTSVESIDIAEDRERFGALAQQHDILVPEHG
                     TGMTEAEAIEVARRIGFPVVVRPSYVLGGRAMAIVYDESNLREYIGYAMEAAPGHPIL
                     IDRFLEDAIELDVDAVADGTDVVIAGVMEHIEEAGIHSGDSTTVIPPYIIGLREQDRV
                     RRLTVQLAKALKVIGLMNVQYAIYHGDLYVLEVNPRASRTVPYVSKATGVPFAKIAAK
                     VMAGKSLRELGLTAEPKVKDYFVKAPVFPFGRFPAEDTVLGPEMKSTGEVMGSAPTFG
                     EAYAKALLAAGIDLPLDGTAFISVNDNDKRPVVVQLAKDLADIGFRVLATSGTRDFLQ
                     HNGVDAGLAFKVHESERPNIVDRMINGEVTLIINTPLGKKSFYDDTYIRRAALQYGIS
                     CLTTLTAATAAVHGIRSLRQGMKTITTIQEQHAR"
     gene            complement(5965..6963)
                     /locus_tag="VNN25_11720"
     CDS             complement(5965..6963)
                     /locus_tag="VNN25_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92241.1"
                     /translation="MIRAKTAKPASKKPAARVGAKAKSAAKKPAPKKPAAKKPASKKA
                     VAKKAVAKKPSPAKAVKKAAKKPASGKAAKTAPKKAAKAAAKTSAKPAAKASAKTSAK
                     PTPKASAAKPAKAEKAADTKQKSAAKGAVKAGAAAPAVARAAAPPADDKKRRRSRTRV
                     TSNGPATAAWFSQGDRPRSSSFIPAPPRAEAPSLVAAPPASSDRLVGAHELTEFAVRT
                     TPVRIDIESSGGRIYLSINPNETNLRAGEGIEWDFRYLGGADVVIEELVIEFAKPSPF
                     NTTVFRSRRPGAARPHRQLSGPAQASAAGKHLQYTVRAFNAFKTEMAVAQPAIVIA"
     gene            complement(7172..>7897)
                     /locus_tag="VNN25_11725"
     CDS             complement(7172..>7897)
                     /locus_tag="VNN25_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92242.1"
                     /translation="FNEPREGGGAASSMYLRRLGEPTPVRIADGYGDAISPDGKYVLG
                     HSGPKLVVVPTGSGESRELKIEGAFDFGAAWLPDGRRIVIAGALPKKGYQLHVIDTLD
                     ETEKAISPENIWGDATRPFAVSPDGRYVAGMTQQQTIALYPVDASAEPSIVPAIQPGE
                     VPIIWSADGASLFVYHPSALPAQVTRVALATGAREVWKQFSPTEPAGVYRIAPICITP
                     DATAYAYDALRMLGDLYVADGLR"
BASE COUNT         1474 a         2327 c         2587 g         1509 t
ORIGIN      
        1 ggcggcttgc gcatcgacac gaaatatccg aagtcgaacg acggcattct cgaatggatc
       61 gccggcacga gcgtgaacct ctcggtgacg tacgaggtcg tggtgccgcg cacgatgagt
      121 ctgcagatcg agaatacgaa cggaggcgtc gaagcgagcg acgtcagtgg ttcgcatcga
      181 gtggagacga ccaacggtca tatcaggctg gcgcgttgcg ccggcgatct cgatgcggag
      241 acgacgaacg gcgggatcga agcggagctg acctcggtca cggccggaca cgtcgtccgc
      301 ctggagacga cgaatggtgc gatcagggcc cgcctgccgc ggaagctggc cgcgagcctg
      361 gacgcttcga cgaccaacgg cggcatcgag tccgatctac ctgttgctac gcgcggcaag
      421 ctggaaaagc acacgctccg cggcacgatc aacggtggcg ggccggacct ccggctgcgc
      481 acgacgaatg gatcgattca tattcagggg atttgatttc cgggtttcgg aggacgtcgg
      541 gggtcgggtg tcggagcggg ctggtggagt ggcaactttg aacagtgcga tccaactaag
      601 cagcggtgtc atcctgagcc gccgaaggac ggtcgaagga tctcagaatt cgtcgatcgt
      661 gtttgtctag cgcgagcggt cgtgatcaag ggcgttcgct cgcgctgggt aaacacgatt
      721 cgcatagtct gagatccttc gaccgtcctt cggcacttcg tgtccttcgg cggatgacac
      781 gaacggggga ggcgcggttg cttgtggcct cgtacgcggg cgcgcgagct gaccgaaacg
      841 ttgccacccc atccctcacc tcatgatcat cttcttgatc ttgctcacga gatcggatcc
      901 gatgaggccc tcggcggcat ttaacacgcg cttgatggcg acgaggacgt cttcgctgcc
      961 gctccagtgt tcctgaacga cggcggcgtc gacgctgctg aggactttgc tcaggaccat
     1021 gacgccgaag acgaggtcat cgagatatcc gatcggtccc acgatcgcct cggggatcag
     1081 atcgaagggc atgatgtagt aggcgacggc gctgccgagg aggaccttgt ccttgctgtt
     1141 gacgcgcgag tccgtcgtga gacgccacag caggatgaag acgtcgggga cgagcaggag
     1201 gaactctccc gctttgccag ccacgcggct tttccggtcg aggtagctgt gcgtcgtttt
     1261 gcggattcgg tcgtagaaac gcgaggcgcg gtcacgcggc aacgactcgg cctcggcgtt
     1321 gtcggcgatt tcccgagcca gatcctcgtc ttctgccgcg ccgctgaagg gtgtttcatc
     1381 catttgcttt ctccgaagac ggcggaaggt cggctccccc tcgcccgagg gtaaccgcca
     1441 gaccgccgtc gatgatccag atgttctcga tatccgcatc gacgaatgat ggcaaaaacc
     1501 gctccaacga aacgacgaac gtggccggct cctcatagtg aacgagatcg gccggcagct
     1561 tgttcttggc gagcccgaaa aggaactgcg gaatcggcag cgcgccgaat gcagaggccg
     1621 aggcgatggg gacgcgcacg gttcgtccgc tggccctgat ctccttaatg tcgaccgtga
     1681 acggcacgct gatgaacttg cggatcgtgc cggagacgcg aagcagcccg ttgaagaatt
     1741 gcagctccat ccgctcgatg ggatcgacgc gaaagccggc cgccatggcg ttgatttttt
     1801 cgccatcgac gcggacttcg agttggtccc aggttatgaa gcgttgcatg tgagaagcag
     1861 agatcagaga tcagcggtca gagatcagga aacagagatc cggggcagag aacgcaattg
     1921 ggtcattcct tactgctgat cgctggccac tgacagccac tgatctctaa tctctgttcc
     1981 ctgatctctg ccctgatcac ttgtatcatg cgcgcgcctc atgccgaaac gtactgacat
     2041 tcactccatt ctcatcatcg gatcgggccc gatcgtcatc ggacaggctt gcgaattcga
     2101 ctactccgga acgcaggcct gcaaagcgct gaaggacgag ggctaccgca tcatcctcgt
     2161 caattcgaat ccggccacga tcatgacgga cccggagttt gccgaccgaa cgtacatcga
     2221 gccgctcacg ccggagacgc tggagcgcat catcgagcgc gaaaaacccg acgcgattct
     2281 gccgaccgtg ggcggccaga ccggcatcaa cctggccatg gccctgcacg agaacggcgc
     2341 gctggagcgc cacggcgtcg agctgatcgg cgccaacagc ctggccatcc ggaccgccga
     2401 agacaggcag ctcttcaaag acaagatggt cgagatcggc gtggaagtgc cgcgctcggt
     2461 gcatgcggcg acggaagcag atgctgagga gtttgcggca acgatcggct atccattagt
     2521 cattcgaccg tcgttcaccc tcggcggatc cggcggcggg atcgcctaca acgtcgagga
     2581 attgcgcgcc gtcgtcagcc acggcctgga gctctcgccg attcatcgcg tcctcgtcga
     2641 ggagtcggtg accggttgga aagagttcga gctcgaggtg atgcgcgaca aggcggacaa
     2701 cgtcatcatc gtctgcagca tcgagaacct cgatccgatg ggtgttcaca cgggcgactc
     2761 gatcacggtt gcgccgcagc agacgttgac gaacaccgag taccaggcca tgcgcgatga
     2821 agccaaacgg atcattcgcg ccatcggcgt cgagacgggc gggtcgaaca tccagttcgc
     2881 cgtgcacccg acgacgggac gccgcctggc catcgagatc aatccgcgcg tgtcgcgctc
     2941 ctcggcactg gcgtcgaagg cgaccggctt tccgatcgcg aagtttgccg cgaagctggc
     3001 ggcgggctac acgctcgacg agatcccgaa cgacatcacg aagaagacgc cggcatcgtt
     3061 cgagccggcc atcgactacg tggtggtgaa gattccacgc ttcgccttcg agaagtttcg
     3121 caagaccaaa ccgaacctcg gcctgtcgat gcaatccgtc ggcgagtcga tggccatcgg
     3181 ccggacgttc aacgaggcgt tgcagaaggg cctccgctcg ctggagatcg gcgtgtcggg
     3241 cctggagtcg cgcggctacg accgcgacaa agcggcatcg ctgctgcgca cgccgcagcc
     3301 ggagcggatc ttcgcgatcg gcgatgcatt ccgcgccggg atgaccgtgg acgaggtgaa
     3361 cgccatctcg tcgatcgatt gctggtttct gcgcgagatc tacaagatcg tggagatgga
     3421 gaagtcgatc accgggaacc ctacggcgaa atcgctgctc gcctggaagc gcgaaggctt
     3481 ctcggacaag cagatcgctt cactgacgaa tctgacagag ggtgaagtgc gcggtcagcg
     3541 ttacgcgctc ggtgtccgtc cggtttacaa gcgcgtcgat acctgcgccg ccgagttcga
     3601 agcgtcgaca ccgtatctct actcgacgta cgagaaggag aacgaggcca acccgacctc
     3661 gcgccgcaag gtgatgattc tcggatcggg tccgaaccgc atcggacagg gcatcgagtt
     3721 cgacacctgc tgcgtacacg cgtcgtttgc gttgcgggag atcggcttcg agaccatcat
     3781 ggtcaactgc aatccggaga cagtgtcgac ggactacgac acgtccgacc gtctctactt
     3841 cgagccgctc acgctcgagg acgtgctcga gatcgtcgat ctggaaaagc cggaaggcgt
     3901 gatcgtccag tttggcgggc agacgccgct gaagctggcc aagtcgctcg ccgaagccgg
     3961 cgtgccgatg tggggcacgt ctgtcgagtc gatcgacatc gccgaagacc gtgaacgttt
     4021 cggagcgctg gcgcagcagc acgacatcct cgtgcccgag cacggaacag ggatgaccga
     4081 ggcggaggcg atcgaggtcg cgcgccgcat cggatttccc gtcgtcgtcc gcccttccta
     4141 cgtcctcggc gggcgggcca tggccatcgt gtacgacgag tcgaatctgc gcgaatacat
     4201 cggctacgcg atggaagcag cgccgggaca tccgattctc atcgaccgct ttctggaaga
     4261 tgccatcgag ctcgatgtcg acgccgtcgc cgacggcacc gacgtcgtca tcgccggcgt
     4321 gatggagcat atcgaggagg cggggattca ctccggcgat tcgacgacgg tgattccgcc
     4381 ctacattatc gggctgcgcg agcaggaccg cgtgcgccgt ctcacggtgc aactggcgaa
     4441 agcactgaag gtgatcggcc tgatgaacgt gcagtacgcg atctaccacg gcgaccttta
     4501 cgtgctcgag gtcaatccgc gcgcctcgcg aacagttccg tacgtgtcga aggcgaccgg
     4561 cgtgccgttc gcgaagatcg cggcgaaggt gatggccggg aagtcgctgc gcgagttggg
     4621 actgactgcc gagccgaagg tcaaagacta cttcgtgaaa gcgccggtgt ttccgttcgg
     4681 acgtttccca gccgaggata cggtgctcgg gccggagatg aagtcgaccg gcgaggtgat
     4741 gggctccgct ccgaccttcg gcgaggcata cgcgaaggcg cttctcgcgg ctgggatcga
     4801 tcttccgctc gatggcaccg cattcatctc cgtcaacgac aacgacaagc gtccggtggt
     4861 cgtgcaactg gcaaaggatc tggccgacat cggattccgc gtcctggcca cctcgggcac
     4921 gcgcgatttt ctgcagcaca acggagtcga tgccggcctc gcgttcaagg tgcacgagtc
     4981 ggaacgtccg aacatcgtcg atcgcatgat caacggcgag gtgacgctca tcattaatac
     5041 accgctagga aagaagtcgt tctacgacga cacgtacatt cgccgcgcag cgctgcaata
     5101 cggaatcagt tgcctgacga cgctgaccgc cgccactgcc gccgtccacg gcatccgctc
     5161 gttacggcag ggaatgaaga cgattacgac gattcaggag caacacgcga ggtaattgcg
     5221 ctgcgtgcgt tgtcggttgt cggttagatg cgcttcgcgc gttgtgaagt tgctggcagt
     5281 tgctggcagt accttctcaa tcgagcgcct tctccccccg caaaaagcgc ggggggagaa
     5341 ggcgctcgat gggtgaagca cgccaaagaa aaaggacaag gtgtagcaag aaggtgcaag
     5401 cgtgtgagaa atgaggggct atccccgggc cgggctaacc ccatttctca caacctcttc
     5461 agcgcctcct tttgcgtcga gcgccttctc cccacggttc ttgtggggga gaaggtggcg
     5521 aagccggatg agggggcgtc ccaacgagca cgacctctgc gcatgtgccg cgttctcaag
     5581 cagttcgggc gaggtgacgc cgatcgttgc ggtaacagtg ccgccgtgaa gggatcgtca
     5641 aaatggttcg gtcacgttcg ttcgcagctc gataacgttg aagacgatct tgccgtcaac
     5701 agcgagaatc ttgccgtcaa cagcgagaat cttgctgttg attgcgagaa tctagctgtt
     5761 aactgcgaga atcttgctgt tgattgcgaa aatctcgctg ttgattccga gaatctcgct
     5821 gttgttggcg agaatctggc tgacgatgac gacgttctcg aagacgttga cgacaaattc
     5881 ggtggaaccg gcgagaaaga gtttccacga ttggttgtat ctgtcgataa tcgccggcta
     5941 accgacaacg cgcgaagcgc aatgtcacgc gattacaatc gccggctgcg ccacagccat
     6001 ctcggtcttg aaggcattga acgcgcgaac cgtgtactgc aggtgctttc cggctgccga
     6061 ggcttgggcg gggccggaga gttggcggtg cgggcgggcg gcgccggggc ggcgtgagcg
     6121 gaagaccgtg gtgttgaacg gcgagggctt ggcgaactcg atgacgagct cctcgatgac
     6181 gacgtcggcg ccgccgaggt agcggaaatc ccactcgatg ccttcgccgg cgcggagatt
     6241 cgtctcgttc ggattgatcg aaaggtagat acgtccaccg ctcgattcga tgtcgatgcg
     6301 caccggtgtg gtgcggactg cgaattctgt cagctcgtga gcgccgacga gacgatcgga
     6361 cgacgctggt ggcgctgcga cgagcgacgg tgcctcagcg cgcggaggcg cgggaatgaa
     6421 cgacgacgag cgagggcggt caccctgcga gaaccaggcg gcggtggccg gaccgttcga
     6481 ggttacgcgt gtacgcgagc ggcggcgctt cttgtcgtcg gcgggtggtg cggcggcacg
     6541 tgcgactgcg ggtgcggcgg ctccggcttt gacagcacct ttggcagcgg acttctgttt
     6601 ggtgtcagcg gctttctccg ctttcgcggg cttggcggcg gaggctttcg gcgtcggctt
     6661 tgccgaggtc tttgcggacg ctttggcagc cggtttggcg gacgttttcg cagccgcctt
     6721 cgcggccttc ttcggggcgg tctttgctgc ttttccggaa gctggtttct tcgcggcttt
     6781 tttcacagcc ttcgcaggcg atggcttttt cgcgacggct ttcttcgcga cggctttctt
     6841 cgaagccggc ttcttcgcgg ctggtttttt cggagcgggc ttctttgcag ccgacttcgc
     6901 tttcgcgccg acgcgcgcgg ccggtttctt cgacgccggc ttcgcggttt ttgctcggat
     6961 cacgggtttc ttgcccgtac cttttttcgg agttgtagac gcttttttcc cctgcacctt
     7021 cgccattcga gcctgctctc ccgcatcccc tgaaaaatgg atggcgcagt atagcggcga
     7081 cccctcggtt tgcataggtg agagaaaagg ccggtttttg gcgtccatca gcctcgaaaa
     7141 gcggcaggcc gcgccgcctc tgccgacgcg gttagcgcaa tccgtcggcg acgtacagat
     7201 cgccgagcat ccgcagggcg tcgtaggcgt aggcggtggc gtcgggagtg atgcagatcg
     7261 gcgcgattct gtacacgccg gccggctcgg tgggcgagaa ctgcttccac acttcccgcg
     7321 cgccggtggc cagtgccacc cgcgtcacct gggcggggag agcgctgggg tgatacacaa
     7381 agagagatgc cccgtcggcc gaccagatga tcggcacttc gcccggctgg atcgcgggga
     7441 cgatcgacgg ctccgcgctg gcgtcgacgg gataaagcgc gatcgtctgc tgttgcgtca
     7501 ttccggcgac gtagcggccg tccggcgata cggcgaacgg acgggtggca tcgccccaga
     7561 tgttctccgg cgagattgcc ttttctgtct cgtcgagcgt gtcgatgacg tggagctgat
     7621 agcccttctt gggcaacgct ccggcgatga cgatccggcg cccgtcgggg agccaggcgg
     7681 cgccgaagtc gaaggcacct tcgatcttga gctctcgcga ctcgccgctt ccggtcggga
     7741 cgacgacgag cttcggcccg ctgtggccga gaacgtactt gccgtcgggc gagatggcat
     7801 cgccgtatcc gtcggcgatc cggaccggag ttggctcgcc gaggcggcga aggtacatcg
     7861 agctggccgc gccgccgcct tcgcgcggtt cattgaa
//
LOCUS       DATLVW010000389         6912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_306263, whole genome shotgun sequence.
ACCESSION   DATLVW010000389 DATLVW010000000
VERSION     DATLVW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306263"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VNN25_11730"
     CDS             complement(<1..1684)
                     /locus_tag="VNN25_11730"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HXH92243.1"
                     /translation="MLGSTFNPQGLTITITPTADLITVQGTVEAALVAQIAPGTSYDP
                     ANGPSAGTTLNAASWAYYWVSSDGSESDWAAVSGSSVPAFTSFTSATAQSIMLPSIAQ
                     VVSGRVYFGMPSLPVIVVASSVPQPVIPTTDPNVYDFVELSYGAGGLTYADTTMIDQF
                     GIPMQISLVPLSPATTAPPTAGVYLDRDDVISQFQSSAGSAFQTGLQNICGQPMVSPQ
                     PRILSPNDVLVYGCVRGVSASVPAGGGSSTLAAGTYYYAVSAVDANGNEGYVQSTLAP
                     ITIAANQQVTIAWSPLASQPYAGAGIASYNVYRYDPLTATWTLLSFSPPLTSSSNAAQ
                     DTGQTGNTKTPNFSPLATWFDAAILSFFTYYQSETLSLVVPNVSPANDTWLYLFGGST
                     VPASGTPESLQFSFTGATDLSGNAIASSDLPFAAGTPFNIDYPYWTSNTYGTSASAPP
                     SWTPYPNSPGSMMVFAANGVWADNMNQVYNNLPPQVPPPPPPPSPLPSTSPAGIWESL
                     LATLENMVVSALTRGIAAPLQNALPIAPASWASQPTQTVPTLSSGTESLSPGT"
     gene            1821..3143
                     /locus_tag="VNN25_11735"
     CDS             1821..3143
                     /locus_tag="VNN25_11735"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HXH92244.1"
                     /translation="MIDSEAEQQIALETGNPGNPVTGRPGQATLQTASIIAIGSEMLG
                     PTRVDTNSLKVTAAFESFGIGVVRKTVVGDRQEELVDEIRHSLAHSSILITSGGLGPT
                     EDDMTRDALAEALGLKMVIDDGIVERLEKRFAARGWVMPEVNKRQANVFIGQTMLPNE
                     RGTAPGFHIEKDGKHIWVFPGVPHELEWMVAEYLVPWLASITAGQARFRRVLKIAGMT
                     ESGVEEKLKPYYAAHPEPLTILATSGQTELHLAEDGAEAEALAVIASREGELRELFGD
                     KIFGVDDDTLEGVVGRILTERHETVATAESCTGGLLASRITDVPGSSAYFMGGAVCYT
                     EEAKKDLARVDPELIREHGEVSEPVAIALARGARERFGTTYGIGVTGIAGPGGGTEAK
                     PVGTVHIAVAGPTGHKHRKLFWPIERTLFKRITTQSALDLLRLFIVRT"
     gene            complement(3431..4744)
                     /gene="rseP"
                     /locus_tag="VNN25_11740"
     CDS             complement(3431..4744)
                     /gene="rseP"
                     /locus_tag="VNN25_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXH92245.1"
                     /translation="MQNIVTNLLGFVLVLGFLIFAHESGHFFVAKLFRVRVLVFSFGF
                     GKRLFGFQRGATDYRVSLIPLGGYVRMAGDTPEENQTGSPDEFLSRPKWQRFLILVAG
                     PVMNVLIAIAFLALISMVGTESFIARPIIGAVTPGKPAARAGLLPGDRIVAMNGEKIE
                     DFNDMRLAIGMHGGSPLRVDYVRNGQTRTTTMTPQKEDSEFGPVGRAGILPYYDTAVG
                     RVTPGSPAAMAGIRTGDHIAAINGKPVTQLAELDAILEATKGAAAGLDVARGNTVFHT
                     TLAAAKPDPNDPYRGFLPPTEIHKLSFFPAVQDSVAQNWKMLRYTFITLGRLFRAEGS
                     VKEFSGPISIARISGEVVRHGWIQTIGLMAMISMQLGIMNLLPIPVLDGGHIMILLIE
                     GVARRDLSLRVKERIQQAGFAVLAALMLVVLYNDVITNVLLMRKG"
     gene            complement(4824..5996)
                     /locus_tag="VNN25_11745"
     CDS             complement(4824..5996)
                     /locus_tag="VNN25_11745"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXH92246.1"
                     /translation="MRSISILGSTGSVGRSTLDVVDAFPDALRVVGLAAGGNLDLLAA
                     QIERYAPELVSVRNPDDVAALRSRVKRRVEIVCGPEGASAVASMPGADAVIAAIVGAA
                     GIPPVYAALKAGKRVGIANKEVLVAAGDVMTRAAAGNGGEILPVDSEHNAVHQALRCG
                     SHAEVQRIVLTASGGPFLRRDLGTFAHITVADALAHPTWKMCNKISIDSATMMNKGLE
                     VIEAHHLFSMPPSAIDILIHPQSIVHSMVEYVDGSFIAQLSTTDMKFPIQYALLYPER
                     VAAPFARLDLAKIRSLEFTAVDAGRFPAVELAYAACRAGGSMPAVLNAANEIAVERFL
                     AGELPFTGIVEIVSRVLDRHAGRTAPITTVEDALAWDGWARKEARTIVVAEKQSIR"
     gene            complement(6138..>6912)
                     /locus_tag="VNN25_11750"
     CDS             complement(6138..>6912)
                     /locus_tag="VNN25_11750"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXH92247.1"
                     /translation="VGWANPYVFDAAIALIAALALYEFLVLGERKGYRIPVALCIGIM
                     MFIVAAFILESVSVEMGVFVALLLIPASYVVSRVDLEKALPSSAIAVLATLYVGMLGG
                     SLIRLHDDFADGAKLVFFLLLVVWLGDAGAYYFGRTFGKHKLSPRISPKKTVEGLAGG
                     VATSVATALVIHFTFFRNFPLVHTIIAGIVLSVAGVVGDLAESMWKRSADVKDSGTLI
                     PGHGGFLDRFDSIFYTTPILYAYWFLIQHDFKSFKIMSA"
BASE COUNT         1341 a         1958 c         2402 g         1211 t
ORIGIN      
        1 aggttcccgg cgagagcgat tcggtgccgg agctgagcgt cggcacggtt tgcgtcggct
       61 ggctggccca gctggccgga gcgatcggca gcgcattctg gagcggcgcg gcgatgccgc
      121 gggtgagggc cgagacgacc atgttttcaa gtgttgccag gagcgactcc cagatgccgg
      181 cgggggaggt cgatggcaga gggctcggcg gcggcggtgg cggcgggacc tgcggcggga
      241 ggttgttgta gacttggttc atgttgtcgg cccagacgcc gtttgcggcg aagaccatca
      301 tcgatccggg cgagttcgga tagggcgtcc acgacggagg ggccgaggcg ctcgttccgt
      361 acgtgttcga tgtccagtac ggatagtcga tattgaacgg tgttccggcc gcgaagggga
      421 ggtcgctgga ggcgatcgcg ttgcccgaca ggtcggtggc tccggtgaac gagaattgga
      481 gagattccgg ggtgcctgag gcgggcacgg tacttccgcc gaagaggtac agccaggtgt
      541 cgttcgccgg cgagacgttc ggaacgacga ggctcagggt ctcggactgg taataggtga
      601 agaagctcag gatggctgcg tcgaaccaag tggccagcgg gctgaagttg ggcgtcttgg
      661 tattgccggt ctggccggtg tcctgtgccg cgtttgagct ggaagtgagc ggtggcgaga
      721 aactcaggag cgtccaggtc gcggtcaagg ggtcatagcg atagacgttg tagctggcga
      781 ttccggcgcc ggcgtacggt tggctggcca gcggcgacca ggcgatcgtg acctgttgat
      841 tcgccgcgat cgtgatcggg gcgagcgtcg actgcacgta gccctcgtta ccgtttgcat
      901 ccactgccga gacggcgtag tagtacgtgc ccgctgccag cgttgacgat ccgccgcctg
      961 ccggaacgct cgcactcacc ccccgcacgc agccgtacac cagcacgtca ttcggggaga
     1021 ggatacgcgg ttgcggcgag accatcggtt gtccgcagat gttctgcagt ccagtttgga
     1081 aggccgagcc tgcgctcgat tggaactggc tgatcacgtc gtcgcggtca agatagactc
     1141 ctgccgtggg gggagccgtc gtcgccggcg agagcggcac gagcgagatc tgcatcggga
     1201 tgccgaactg gtcgatcatc gtggtgtcgg cataggtcaa gccgccggcg ccgtaggaga
     1261 gctcgacgaa gtcgtacaca ttggggtcgg tggtcgggat caccggttgc gggacgctgc
     1321 tggccaccac gatcacgggc agcgacggca tcccgaaata gacacggccg gacacaactt
     1381 gggcgatgct cggcagcatg atgctctgtg ccgtggccga ggtgaacgag gtgaacgccg
     1441 gcacgctcga tccgctcacc gccgcccagt cggactcgct gccgtcggag ctgacccagt
     1501 aataggccca cgaggccgcg ttcagagtcg tccccgcaga cggaccgttc gccgggtcgt
     1561 aactcgtccc gggagcgatc tgcgccacca gagctgcttc gacggttcct tgcacggtga
     1621 tgagatcggc ggtaggggtg atcgtgatcg tcagtccctg tggattgaac gtcgaaccaa
     1681 gcatgtgctt cctccaccgc taggagagtg ttccgcgcgc agaatcccta ccctcgagga
     1741 tcagcagggc cgaagttgat cgagcagccg ccgcctaacg gttgccaggg tacagggttg
     1801 gtgagtagtg tgcgagctgc atgatcgact cagaagcgga gcaacagatc gcgctggaga
     1861 cggggaaccc gggcaacccg gtaaccgggc ggccaggcca ggcaaccctg caaactgcca
     1921 gcatcatcgc catcggttcc gagatgctcg ggccgacgcg cgtcgatacg aactcgctca
     1981 aggtcaccgc cgcgttcgaa tcgttcggca tcggtgtcgt gcgcaagacg gttgtcggcg
     2041 acaggcagga ggagctggtc gatgagatcc ggcactcgct cgctcactcc agcatcctga
     2101 tcacctccgg cgggctgggg cctaccgaag atgacatgac gcgcgatgcg ctggccgagg
     2161 cgctggggct gaagatggtg atcgacgacg gcatcgtaga gcggctggag aagcgcttcg
     2221 cggcgcgcgg ctgggtgatg ccggaggtca acaagcgtca agccaacgtt ttcatcggac
     2281 agacgatgct tccgaatgaa cgcgggacag ctcccggctt tcacatcgag aaggacggta
     2341 agcacatctg ggtatttccg ggcgtgccgc acgagctgga gtggatggtg gcggagtatc
     2401 tcgtgccgtg gctggcttcg atcacggcgg gacaggcacg gttccggcgc gtgctgaaga
     2461 ttgcagggat gacggagtcg ggcgtcgagg agaagctgaa gccttactac gcggcgcatc
     2521 cggagccgct gacgattctg gctaccagtg gccagacgga actgcatctg gccgaggacg
     2581 gtgcggaggc cgaggcgctg gcggtgatcg catcgcgcga gggcgagttg cgcgagctgt
     2641 tcggcgacaa gatcttcggc gttgacgatg acaccctcga aggtgtggtc ggccggatac
     2701 tcaccgagcg tcacgagacc gtcgcgactg cggagtcgtg cacgggcggt ctgctggcct
     2761 ctcgtatcac cgacgtgccg ggatcgtccg cgtacttcat gggcggcgcg gtctgctaca
     2821 cggaggaagc gaagaaggat ctggccagag tcgatccgga gttgatccgc gagcatggcg
     2881 aggtttcgga accggtggcg atcgcgctgg cgcgcggcgc gcgcgagcgg ttcggaacga
     2941 cgtacgggat tggcgtaacc ggcatcgccg ggccgggtgg tgggacggag gcgaagccgg
     3001 tcgggaccgt ccatatcgcg gtggcgggtc caacaggaca caagcatcgc aagctgttct
     3061 ggccgatcga acggacgctc ttcaagcgca ttaccacgca gtccgcgctc gacctgctgc
     3121 gattgttcat cgtgcgcacg tagcgtacgc ttcctgtgta tgaagcgcat gaacatagta
     3181 atcgatcgcg aagcgttgga gttggcgaga cggctgaccg ggaagaagac ctactccgag
     3241 acgatcaacc acctcatcgc gcaagctgtg aggcgggaga aactgtgaat ggccatcgat
     3301 gcgttggcca aagacccgga cccatgatgg cccggatatg tcgaggaaat ggctcccgac
     3361 gtcgccgagt atctcaggaa aacaaagaag ccaggccgtc atgctgcgcg aaaagtgcgc
     3421 gcgcggaacg tcatcccttc cgcatcaaca gcacgttcgt gatcacgtcg ttgtagagga
     3481 caacgagcat caaggcggcc agtacggcga aaccagcttg ttggatgcgc tccttcacgc
     3541 gtagcgagag gtcgcggcgc gccactcctt cgatgagcag gatcatgatg tgcccgccgt
     3601 cgaggacggg gatcgggagc aggttcatga ttccgagctg catcgagatc atcgccatca
     3661 gaccgatcgt ctggatccag ccgtgacgta ccacttcgcc cgagatgcgc gcgatcgaga
     3721 tcgggccgct gaactctttc acgcttccct cggcgcggaa gaggcgcccg agcgtgatga
     3781 acgtgtagcg cagcatcttc cagttctgcg cgacgctgtc ctgaacggcg gggaagaacg
     3841 acagtttgtg gatctccgtc ggcgggagga agccgcggta cgggtcattc ggatcgggct
     3901 tggccgcggc cagggtggta tgaaagacgg tattgccgcg agcgacgtcg aggcctgcag
     3961 cggctccctt ggtcgcttcg aggatggcgt cgagctcggc gagttgcgtg accggtttcc
     4021 cattgatggc ggcgatgtgg tctccggtac ggatgccagc catggcggct ggcgagcccg
     4081 gcgtgacgcg tccaaccgcg gtgtcgtagt agggaaggat gccggcgcgg ccgacgggac
     4141 cgaattcgct gtcctccttc tgcggggtca tcgtcgtcgt tcgcgtctgc ccgttgcgca
     4201 cgtagtcgac gcgcagtggt gagccgccat gcatgccgat ggcgaggcgc atgtcattga
     4261 agtcttcgat cttttccccg ttcatcgcca cgatgcggtc gccgggcagg agaccggccc
     4321 gggctgccgg tttgccaggc gtgaccgctc cgataatcgg gcgcgcgatg aacgactccg
     4381 ttccgaccat gctgatcaga gccaggaagg cgatggcgat cagcacgttc atgaccggtc
     4441 cggccacgag gatcagaaag cgctgccact tcggccgtga aagaaactcg tcgggactgc
     4501 cggtctggtt ttcctcggga gtgtcgccgg ccatgcgcac gtaaccgccg agcggaatga
     4561 gcgaaacacg atagtcggtg gcgccgcgct ggaatccgaa caagcgctta ccgaagccga
     4621 atgagaagac gagcacgcgt acgcggaaga gcttggcgac gaagaagtga cccgactcgt
     4681 gggcgaagat caggaagccg agaacgagga cgaagccgag aaggttggtg acgatatttt
     4741 gcatgttcgg tgggttagta gatcagtcga ccggtgcatt tcatagatag acggactgcc
     4801 attgaaatct acgactctac tcatcaacga atcgactgct tctctgcaac aacaattgtc
     4861 ctcgcttctt tcctggccca tccgtcccag gccagggcgt cttcgaccgt cgtgatcggg
     4921 gcggtgcggc cggcgtgcct gtcgagcacg cgcgacacta tctctacgat tccggtgaac
     4981 ggaagttccc cggccaggaa acgctcgacg gcaatctcgt tggcggcgtt gagcacggcg
     5041 ggcatggaac cgccggcgcg gcaggcggcg tacgccagct ccacggccgg gaagcgcccg
     5101 gcgtcgacgg ctgtgaattc gagcgagcgg atctttgcca gatcgagccg cgcgaacggt
     5161 gcagccacgc gctccggata gagaagcgcg tactggatcg ggaacttcat gtcggtggtg
     5221 gagagctgtg cgatgaacga gccgtcgacg tactcgacca tggaatgcac gatcgactgc
     5281 ggatggatga ggatgtcgat ggcggacggc ggcatcgaga agagatgatg cgcttcgatc
     5341 acctcgaggc ccttgttcat catggtggcc gagtcgatgg agatcttgtt gcacattttc
     5401 caggtggggt gagcgagggc gtctgcgacg gtgatgtgcg cgaaggtgcc gaggtcgcgt
     5461 cgcagaaacg ggccaccgga ggccgtgagg acgatccgct ggacctcggc gtgcgaaccg
     5521 cagcgaagtg cctggtgtac ggcgttgtgc tcggagtcga cgggcaggat ctcgccgcca
     5581 ttcccggccg cggcgcgcgt catcacgtca ccggcggcga ccagcacttc cttgttggcg
     5641 atgccgacgc gcttgccggc tttcagcgcg gcgtagacag gcgggatgcc ggccgcgcct
     5701 acgattgcgg cgatcaccgc atcggctccc ggcatcgaag cgacggcaga ggctccctct
     5761 gggccgcaga cgatttccac gcggcgtttc acccggctgc gcagcgcagc aacgtcgtca
     5821 gggttgcgga cggagaccaa ctccggcgca tatcgctcga tctgggcggc cagaaggtcg
     5881 agatttccgc ccgcggcaag accgacgacg cgaagggcat cgggaaaggc atcgacgacg
     5941 tcgagcgtcg aacgcccgac ggagccggtg gagccgagga tggagattga tctcataagt
     6001 tcgtttcgtg gaggtcagag agcagagaca gcagagacaa gagatcagag atatcagaga
     6061 tcagggatca gggatcaggg atcagattat ccggataact tcctgatctc tgatctcttg
     6121 tctctgatct ctgcctttca cgccgacatg atcttgaagc tcttgaaatc gtgctggatc
     6181 aggaaccagt aggcgtaaag aatcggcgtc gtgtagaaaa tcgaatcgaa acggtcgaga
     6241 aagccaccgt gtccgggaat gagcgtaccg gagtctttca cgtcggcgga gcgcttccac
     6301 atcgattcgg ccagatcgcc gaccactcct gcaacggaga gaactatgcc ggcgatgatc
     6361 gtgtggacga gcggaaagtt gcggaagaac gtgaagtgaa tgacgagtgc cgtggcaacg
     6421 gacgttgcca cgccgccggc gagcccttcc acagtcttct tcgggctgat gcggggggaa
     6481 agtttgtgtt ttccgaacgt gcggccgaag tagtacgccc ccgcgtcgcc gagccagacc
     6541 acgagcagga gaaagaagac gagctttgcg ccatcggcga agtcgtcgtg caggcggatg
     6601 agcgaaccgc caagcatccc gacgtaaagc gtggcaagaa cggcgatcgc actcgatggg
     6661 agggccttct ccagatcgac acgcgacacg acgtaggatg ccggtatcag gagcagcgcc
     6721 acaaagactc ccatctcgac cgagaccgat tcgaggatga aggcggcgac gatgaacatc
     6781 atgatcccga tgcagagcgc tacgggaatg cgatagccct tccgctctcc cagcacgaga
     6841 aactcgtaca gcgccagcgc ggcgatcagg gcgatggccg catcaaagac gtacggattc
     6901 gcccagccga cg
//
LOCUS       DATLVW010000390         5752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_427003, whole genome shotgun sequence.
ACCESSION   DATLVW010000390 DATLVW010000000
VERSION     DATLVW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427003"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1465
                     /locus_tag="VNN25_11755"
     CDS             119..1465
                     /locus_tag="VNN25_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92248.1"
                     /translation="MAKILIIDDEASIRETLANILQDEGHQTTLCDSGEEAIAQFARE
                     EYDLVFLDLWLPGIDGMAVLERLKTAGAPPVIVISGHGNIDSAVRATRLGAYDFLEKP
                     LSLERVLLTVNHALADRKLRDQVRDLSQRFTLEELLIGESEPMRKLDEQIRAAAPSNS
                     RVLITGENGSGKEIVARTLHRLSQRAEQPFIDVNCAAIPEELIESELFGHRKGAFTGA
                     IDDRKGKFELADGGTLFLDEIGDMSLKTQAKVLRVLQEQTFQKVGGQQTINVDVRVIA
                     ATNKSLETEIANGSFRSDLYYRLNVVPIEVPPLRMRGHDIVLLAEHFLRRFAAESGSP
                     RKRLSAGAGSKLRAYHWPGNVRELRNVMERLAILMKGDLIEPEDVQLGSASAAPAQID
                     ARLSLKEARDEFEKQYILSRLREFAGNVSRTAEALGVERSNLYRKLHAYGIRVERG"
     gene            1551..1626
                     /locus_tag="VNN25_11760"
     tRNA            1551..1626
                     /locus_tag="VNN25_11760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1584..1586,aa:Gly,seq:gcc)
     gene            complement(1674..2810)
                     /locus_tag="VNN25_11765"
     CDS             complement(1674..2810)
                     /locus_tag="VNN25_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92249.1"
                     /translation="MKIRFALALLILPIITSAQPAPAPPSDGSWRFAVAGDARNCGDV
                     IMPAIAEGAKTNGARFYWHLGDWRAIYNFDEDMAQAAVMAGTPLQVQSYLRGAFKDAL
                     DNQLKPFDDRGVGVFVGIGNHETVWPLSRAAWVKAFTPYLDIAAIRDQRLKDDPSDTT
                     VHTYYHVVTNGLDFITLDNGSCEMFDDAQMAWLKKVLDRDAADASIRTIVAGMHEPLP
                     DGRSCGHSMGNYPIQQKTGREVYAMLLSMRDKSKKQVYVLASHSHFLMDNAYDSDYWR
                     THGGVLPGWIVGTSGAQRYRLPDTVTPGPMNRTDVYGYLLGTVTANGAITFEFREVTP
                     KDIPADVVKKYSKNFIENFCFAANRNMTPQEGNCPPMSQCSITP"
     gene            complement(2894..3997)
                     /locus_tag="VNN25_11770"
     CDS             complement(2894..3997)
                     /locus_tag="VNN25_11770"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92250.1"
                     /translation="MTKRGVVLGILFLLALAPPAMATHIPSSSEVISYAGSLQPGVAA
                     TGTMGWGSTPPKGYTWYCFDVTSGKAVTLNVKKTSGDLKPNLGVMRGLAEAGGTATLP
                     IVKDTSNPDQDQTDLTFTPDFTGPVTLWVSTFLGEKNGVFSVTMTGGTGRSSCGAVDA
                     GPSGPLLAIVPDAPSFAMGNSQTIKVPVQVFAASTVTSDIAINAGGLPDDVHVSIAPN
                     VLAARSDGKATITISTSANTFPKTYLVYLIATSGEQTASNSFVLEIICDPPLILGIDQ
                     PKNTTVALGRAATLSVKASGSGPLTYQWFSGAKGLVNFPLAIGSSPNFTTLAINDTTS
                     YWVRVANPCGSVDSQTVTVSVAPSAKPASSRRH"
     gene            4175..4918
                     /locus_tag="VNN25_11775"
     CDS             4175..4918
                     /locus_tag="VNN25_11775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH92251.1"
                     /translation="MTSKTHAWGTQPEMFGPRHEYRLGIITREVERLPPGARILDAAV
                     GLGQLAGRMQRRGYDVIGIDYSLDAALHVKQTLHIPVVIGDMTKLPFRAGAFTGVTTG
                     ETLEHLDDDAGAVREIARVLAPGGTCIATVPAMQSLWTASDDYYEHRRRYARNQLTSL
                     FRSAAMTIRKAAFWGFPIVLVYDTLVILPMNKRRARMAINDDAALRSVARAGKSRLLV
                     NAVRAFFSIDRIFGFIPFGPGLLLVAKKD"
     gene            4951..5331
                     /locus_tag="VNN25_11780"
     CDS             4951..5331
                     /locus_tag="VNN25_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92252.1"
                     /translation="MADENESAVEDQQPFSKPVEAELTPEERKHRAFQRAIALKGKFH
                     LDIDLDELRGRSRIEARASDVYDGDMANRNQAAVIDRQTENKPVETELTPEERRHRAF
                     QRLQALKGKIHLDIDIDELRGRNR"
     gene            5315..5692
                     /locus_tag="VNN25_11785"
     CDS             5315..5692
                     /locus_tag="VNN25_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH92253.1"
                     /translation="MAVIADTSVLVDYLEGRSSRSMEEAIQAESLVLPPIVIAELLSG
                     SDDPFRRAAVAEVLQDFPMQFVDLGHWMAVGDLRREMKGRGVNLTIPDAHVAQCALDL
                     NATLLTRDAVFALIAEHVPLRLG"
BASE COUNT         1082 a         1688 c         1860 g         1122 t
ORIGIN      
        1 acatcgagaa caatccggtc aaggctgggt tgtgtcggga agccggtgat tggatgtttt
       61 ccagtgcgaa gcgcagaaaa ggagaggacg gcgggcggga cgcccgcgct cccaggtaat
      121 ggccaagatc ctcatcattg acgacgaagc gtcgatccgc gagacgctgg cgaacatcct
      181 tcaggacgaa gggcatcaaa cgacgctctg cgattccggc gaagaggcga tcgcgcaatt
      241 tgcgcgcgaa gagtacgacc tcgtgtttct cgacctctgg ctcccaggca tcgacgggat
      301 ggccgtgctc gagcgtctca agaccgcggg cgcgcctccc gtgatcgtga tctctggtca
      361 cggcaacatc gattccgcgg ttcgcgccac ccgcctcggc gcttacgact tcctcgaaaa
      421 gccgctgtcg ctggaaaggg ttctgctgac cgtgaatcat gcgctggccg accgcaagct
      481 gcgcgatcag gtgcgcgacc tgagccagcg attcacgctc gaagagttgc tgatcggcga
      541 gagcgagccg atgcggaagc tcgacgagca gatccgggca gcggcgccgt cgaacagccg
      601 cgtcctgatc accggcgaga atggcagcgg caaggagatc gtcgcgcgga cgctgcaccg
      661 tttgtcacaa cgcgcggagc agccgttcat cgacgtcaat tgcgctgcga tccccgagga
      721 gctcatcgag tcggagctct tcggtcatcg caagggcgca ttcactggcg ccatcgacga
      781 ccgcaaaggg aagttcgaac tggccgacgg cggcacgctt ttcctcgacg agatcgggga
      841 tatgagtcta aagacccagg cgaaggttct gcgggtgctg caggagcaga cgttccagaa
      901 ggttggcgga caacagacca tcaacgttga cgtccgcgtg atcgccgcga ccaacaagag
      961 tctggagacg gagatcgcga acggtagctt tcgcagcgat ctctactacc gtctaaacgt
     1021 cgtgccgatc gaagtgccgc ctctgcgcat gcgcggccat gacatcgtcc tgctggcgga
     1081 acattttcta cgccgcttcg ccgcggagtc ggggagcccg aggaaacggc tgtctgccgg
     1141 cgcaggctcg aagttgcgcg cctatcactg gccgggaaat gtgcgcgaac tgcgcaatgt
     1201 catggagcgg ctggccattc tgatgaaggg ggacttgatc gagccggagg atgtccagct
     1261 cggctctgcc tccgccgcgc cggcacagat cgatgcccgc ctgtcgctga aagaggcgcg
     1321 cgacgagttc gaaaaacagt acatcctctc ccgccttcgc gagttcgccg gcaacgtcag
     1381 ccgcaccgcc gaggcgttgg gcgtggagcg ttcgaacctc tatcgaaagc tgcacgcgta
     1441 cgggattcgc gtcgagcgcg gttgagcgcg caggcgagga atagctctta cttccagagg
     1501 gttagccgct tgactgcaca cgaaggttca tctaatcttc gccggctttc gcgggaataa
     1561 ctcagttggt agagtgcaac cttgccaagg ttgatgtcgc cggttcgagt ccggtttccc
     1621 gctccaaccg aacctgattt gaagggccgc cgcgaggcgg cccttttttc gcttcacggc
     1681 gtgatcgagc actgcgacat cggcggacag ttgccttcct gcggtgtcat gttgcggttg
     1741 gcggcgaagc agaagttctc gatgaagttc ttcgagtact tcttcacgac gtcggcgggg
     1801 atgtctttcg gcgtgacctc gcgaaactcg aaggtgatcg ctccgtttgc ggtgacggtg
     1861 ccgaggagat agccgtagac gtcggtgcgg ttcatcggtc ccggggtgac ggtatccggc
     1921 agccggtaac gctgcgcgcc cgacgtgccg acgatccatc ccggcagaac gccgccatgg
     1981 gtccgccagt aatcgctgtc gtacgcgtta tccatgagga agtgcgagtg gctggccagg
     2041 acgtagacct gcttcttgct cttgtcgcgc atcgacagca gcatcgcgta cacctcgcgt
     2101 ccggtcttct gctggatcgg atagttcccc atgctgtggc cgcacgagcg cccgtcggga
     2161 agtggttcgt gcatgccggc gacgatcgtg cggatcgatg cgtcggcggc gtcgcggtcg
     2221 agtaccttct tcagccaggc catctgcgcg tcgtcgaaca tttcgcacga gccgttgtcg
     2281 agcgtaatga aatcgaggcc gttagtgacg acgtggtagt acgtatgaac ggtcgtgtcg
     2341 ctcggatcgt ccttcaaccg ctgatcgcga atggccgcaa tgtcgagata gggagtgaat
     2401 gccttgaccc aggcggcacg cgacagcggc cagaccgttt cgtgattccc gattccgacg
     2461 aagacgccga cgccgcggtc atcgaacggc ttgagctggt tgtcgagcgc atctttgaag
     2521 gcgccgcgaa ggtagctctg gacctgaagc ggcgtgccgg ccatcacggc tgcctgtgcc
     2581 atgtcctcgt cgaagttgta gatggcgcgc cagtcgccga ggtgccagta gaaacgcgcg
     2641 ccgttggtct tcgctccctc cgcgatggcg ggcatgatga cgtcgccgca gttgcgcgcg
     2701 tcgccggcaa cggcgaaacg ccacgatcca tcggatggcg gcgccggcgc tggttgcgcg
     2761 gaggtgatga tcggaaggat gagcagcgca agggcaaagc gaatcttcat tgatggtctc
     2821 ctcagagctc cgatcataaa aggaaacgcc cggactttgc cgggcgtttc gtgtttgttc
     2881 gtttgcgcga tgatcagtgg cggcggctcg acgccggttt ggcgcttggt gcgacgctta
     2941 ccgtgaccgt ctggctgtcg acgctcccgc acgggttggc gacgcgaacc cagtacgacg
     3001 tcgtgtcgtt gatcgcgagg gtggtgaagt tgggcgagct tccgatggca agggggaagt
     3061 tcaccagccc cttcgcgccg ctgaaccatt gataggtgag cgggccgctc cccgaagcct
     3121 tcacggaaag cgtcgccgcg cggccgagag cgaccgtggt gttctttggc tggtcgatgc
     3181 caaggatgag gggcggatcg caaatgatct cgagaacaaa actgttggac gccgtctgtt
     3241 cgccgctggt ggcgatgagg tagacgaggt aggtcttagg aaacgtattg gccgaggtgg
     3301 agatggtgat cgtcgctttg ccatcgctac gcgcggcgag aacgttcggc gcaatcgaca
     3361 cgtgaacgtc atccgggaga ccgccggcgt tgatggcgat gtcgctcgtg accgtcgacg
     3421 ccgcgaatac ctgcacgggg acttttatgg tctgcgagtt gcccatggcg aacgacgggg
     3481 cgtcgggcac gatggcgagc aagggcccgg acggaccggc gtcgacggcg ccgcaactcg
     3541 agcgtcccgt gccgccggtc atcgtgaccg agaacacgcc gttcttctcc ccgaggaacg
     3601 tcgagaccca caacgttaca gggccggtga aatcgggggt gaaagtgagg tcggtctggt
     3661 cctggtccgg gttgctcgtg tccttcacga ttgggagcgt ggcggtgcct ccggcttcgg
     3721 cgagacctcg catgacgccc aggtttggct tcaaatcgcc ggaggtcttc tttacgttga
     3781 gcgtgacagc cttaccgctc gtcacgtcaa agcaatacca ggtgtaaccc ttcggcgggg
     3841 tgcttcccca acccatcgtg ccggtagcgg cgacgccggg ctgcaagctg ccggcgtatg
     3901 agatgacttc cgatgatgac ggtatatgtg ttgccatcgc cggcggcgcg agcgccagca
     3961 gaaagagaat gccgagcaca acaccacgtt tcgtcatatc cgccctcaca tccctggagt
     4021 tttaattgat tatgctgagt atagggctac ggagcaccgt tagcgcatcc cgcggtcgct
     4081 gcgggcccag gcagaccgca gtgaacgccc tttccaacat gccaatgact ttgtgcaagt
     4141 atagcggaga taatcgcccg ccgctgactt tgagatgact tccaaaaccc acgcatgggg
     4201 cacccaaccg gagatgttcg gtccccgcca tgagtaccgg ctcggcatca tcacgcgtga
     4261 agtggagcgg ctgccgccag gagcgcgaat ccttgatgcc gcggtcggcc tcgggcaact
     4321 cgctgggcga atgcagcggc gcgggtacga cgtcatcggc atcgattact cgctcgatgc
     4381 ggcgctgcac gtcaaacaaa ccctccacat accagtcgtc atcggggaca tgacgaagct
     4441 gccgtttcgt gccggtgctt tcaccggggt gacgacaggg gagacgctcg agcatctcga
     4501 cgacgatgcc ggcgccgttc gcgagatcgc gcgcgtcctg gcgccgggtg gaacctgcat
     4561 cgctacggtc ccggcgatgc aatcgctctg gacggccagc gacgattact acgagcatcg
     4621 ccgccgctac gcgcgcaacc agctcacgtc gctgtttcgg agcgcggcga tgacgatccg
     4681 gaaagccgcg ttctggggtt ttccgatcgt gctcgtctac gacacgctcg tgattctgcc
     4741 gatgaacaag cgccgagcgc gcatggcgat caacgacgat gccgcgctcc gttcggtcgc
     4801 gcgcgcgggc aagtcacggt tgctggtgaa cgccgtccgc gcgttcttct ccatcgaccg
     4861 gatcttcgga ttcatcccgt tcgggccggg actgctgctg gtggcgaaga aggattgaag
     4921 caggggctgt gtagtgttga tgatggtgcc atggccgatg aaaacgaatc cgccgtcgag
     4981 gatcagcagc ctttcagcaa acccgtcgaa gctgagttga cccctgaaga gcgcaagcat
     5041 cgggcgtttc agcgcgccat tgctttgaag gggaagtttc atctggatat tgatctcgac
     5101 gaacttcgcg gccgttcgag gattgaagcg cgagcctctg acgtttatga tggcgatatg
     5161 gcgaatcgga accaagccgc agtcatagac cggcagacgg aaaataagcc tgtcgaaaca
     5221 gagttgacgc cggaagagcg gcggcatcgt gctttccagc gcctccaggc gctcaaaggc
     5281 aagatccacc tcgatatcga tatcgacgag ctccgtggcc gtaatcgctg atacgtccgt
     5341 tctggtcgat taccttgaag gccgcagttc gcggtcgatg gaggaagcga tccaggccga
     5401 gtcgctcgta cttccaccga tcgtgatcgc ggagctcctt tccggttctg acgatccttt
     5461 tcgccgagct gccgtagccg aggttttgca ggactttccg atgcaattcg ttgacttggg
     5521 acattggatg gcagttggcg accttcgtcg cgagatgaag ggacgcggcg taaatctcac
     5581 gatccccgac gctcacgtcg cccaatgcgc gctcgacctc aacgccacgc ttctcacccg
     5641 cgatgccgta tttgctctca tcgcggaaca tgtcccgctc cggcttggct gatccgaacg
     5701 cgatccggta gagtggcctg ctgattgaat cgagatggaa cctgagatga aa
//
LOCUS       DATLVW010000391         4935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_594867, whole genome shotgun sequence.
ACCESSION   DATLVW010000391 DATLVW010000000
VERSION     DATLVW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594867"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VNN25_11790"
     CDS             <1..965
                     /locus_tag="VNN25_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH92254.1"
                     /translation="WAYAASDSSHVNEIFTGPATGPARKLTDMTAQFAGMNLGSVELI
                     SWPSKDGTIIEGVLHKPPNFDASKKYPLLVVIHGGPTGVSRPVFSPANRTYPIELFLA
                     KGALVLEPNYRGSAGYGAKFRALNVRNLGVGDMWDVMSGVDALIARGIADGTKLGAMG
                     WSQGGYISAFLTTNTDRFKAISDGAGISDWTTYYVNTDITPFTRQYLQATPWDDPKVY
                     ALTSPITNIRNAKTPTLIQHGEKDKRVPPPNAFELYRGLRDAGVPSTLIVYAGFGHGI
                     NKPKSNRAVLQHNYDWFSHYVFGEEIPKDSPLRGSSAIVDHSGE"
     gene            1343..1413
                     /locus_tag="VNN25_11795"
     tRNA            1343..1413
                     /locus_tag="VNN25_11795"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1815..2921
                     /locus_tag="VNN25_11800"
     CDS             1815..2921
                     /locus_tag="VNN25_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXH92255.1"
                     /translation="MFHRYKINPHERGLLFKDGDLVTVLGPGAHWYLDPLMKLRLHVV
                     SPRVNPWLVHPSLDLIARSGMLGSEATVIDLNDHQRGLVWIDKRFARLLAPGLYVLWN
                     VEHEVRVEIVDARSMQLVHADLPVIMKGSGANELLEIVNVEAGHNGVWFRDGAYQATL
                     GPGAWVFWKGTGRLKVIDVDLREQTLDISGQEIMTADKVTLRLNALVVYRVTDPLRSV
                     AEVDGAGQALYRAAQLALREIVGARELDVLLVSKEALATELSELTKARAGELGLTVLS
                     TGIRDVILPGDMKDLMNKVMEARKAAEASLISRREETASMRMQANTAKILESNPTLMK
                     LKELEVLEKVADKANLTVLLGGESGLNERVMKLL"
     gene            complement(2960..3277)
                     /locus_tag="VNN25_11805"
     CDS             complement(2960..3277)
                     /locus_tag="VNN25_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92256.1"
                     /translation="MNRELYFTPEARRNLADLQKSAASRGTLKQVRKTLGLLETNLRH
                     PSLNTHPFRSLHGPSGEEVFEAYAQNETPGAYRVFFMYGPDRMEGKNRVAVLTIIAIT
                     PHP"
     gene            complement(3274..3573)
                     /locus_tag="VNN25_11810"
     CDS             complement(3274..3573)
                     /locus_tag="VNN25_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92257.1"
                     /translation="MKAARRSGYRLLARDVRPDSRKRVALGRALDDIGDASFNVYKDD
                     EGRIILEPHISIPASEAWLFRNQKAIDSVRRGLEEAAGGKAKRGASFAQFADDDE"
     gene            complement(3697..>4935)
                     /locus_tag="VNN25_11815"
     CDS             complement(3697..>4935)
                     /locus_tag="VNN25_11815"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HXH92258.1"
                     /translation="VGTSGATVTAGTSSTTSDGSNNYTMAGFVAGTYTVTPSKSGCTF
                     SPASSSVTITSSNAVANFTATCGSGDTQLTSGVALTGQSVALNAWKYYYITVPAGATN
                     LTFATTSATSDVDIYTQFNAKPTLTTYVCRPYTSSGNETCSATNPSAGTWWVGVNGYT
                     AGNFTITGTVTTPVATYSISGSAGTPGATVTAGSQGATSDASNNYSIANLANGTYTVT
                     PSKSGCTFTPTSSSVTIAGANVTGTNFTASCTGPTTLFSNGFESAGTGWATAQVSGTA
                     GAWTFVTAGTLPTASPHGGSYLALFNSYDSASGSQTRVYQTTGFAIPSSATTVTLKFW
                     MYHDTGYSTSADRVQVQVSTGTWASVGTAINRYDGSTGWKQHTIDLTAYKGTTVQLGF
                     LGISAFGNNVFIDDVIVTTP"
BASE COUNT          919 a         1480 c         1595 g          941 t
ORIGIN      
        1 gctgggcgta tgcagcttcc gattcttcgc acgtcaatga gatcttcacc ggaccggcga
       61 ccggtcccgc acgcaagctc accgacatga ccgcacaatt cgccggcatg aacctcggca
      121 gtgtcgaact gatctcgtgg ccgagcaagg acggcacgat catcgagggc gtcctgcaca
      181 agcctccgaa cttcgacgca tcgaagaagt acccgttgct cgtggtcatc catggcggac
      241 cgaccggcgt atcgcgcccc gtgttcagcc cggccaaccg cacgtatccg atcgagctct
      301 ttctggcgaa aggtgcgctg gtgctcgagc cgaattaccg cgggagcgcc ggctacggcg
      361 caaagttccg cgccctcaac gtccgcaacc tcggcgtcgg cgacatgtgg gacgtcatga
      421 gcggcgtcga tgctctgatt gcgcgcggaa tcgccgacgg aacaaagctc ggcgcgatgg
      481 gttggtcgca gggcggctac atctccgcat tcctgaccac gaacaccgat cgtttcaaag
      541 cgatctccga cggggccggc atcagcgact ggaccacgta ctacgtcaac accgacatca
      601 ccccgtttac gcggcagtat ctgcaggcaa cgccgtggga cgatccgaag gtttacgcgc
      661 tgacctcgcc gatcaccaac atccgcaacg caaagacgcc aactctgatc cagcacggcg
      721 agaaggacaa gcgcgtacct ccgccgaacg cgttcgagct ctatcgcgga ctgcgcgacg
      781 ccggcgtgcc cagcaccctg atcgtctatg ccggcttcgg tcacggcatc aacaagccca
      841 agtccaatcg tgcggtgctc cagcacaatt atgactggtt cagccactac gtcttcggcg
      901 aggagatccc gaaagactcg ccgctgcgcg gcagcagcgc gatcgtcgat cacagcggcg
      961 agtgagccct taaaggtgtc ttttgctatt gaaaagttac gccataagaa aaaacatgtt
     1021 gactccacgc atatagcaat gctattttcc gccgccctat gaaactcatg gatcaattga
     1081 ttgaacgcgc acagcgcagc tcctggcctt cgggtcttgg ctgcagattt gcgccggagg
     1141 tgcctcgaat cgggtagaga accgcacatc tacctcattg cgaggcccct tcgaaccgtc
     1201 tggtcgaagg ggctttttgt ttctttgtgc gattgcaaca taggcgataa gccgtccggg
     1261 ccggcggcgg gcatccgtgt catgacgaca ttcgggattg cctgttccgt gaacgggttc
     1321 tgacgatgga ggtttgccgc actaacgtgt agctcagtgg atagagcaca ggaccgggat
     1381 cctgcaacgc gggttcgact cccgtcacgc taacaaaggc ctcgaaagcc ttttcataca
     1441 acgactgacc gccgtaagtg gtcaaccggg cgacggcggg attcgtcccg ccggccaaag
     1501 gctccggcct gaggcaaacg gaaagcacgc gccttgaggc gatcgcgagg cacgcgacaa
     1561 agcagcgcag acgacatcgt cgagcggaag agcgggtggc ttccacaaga cgatgctccg
     1621 agcactgccg agcgcgcagc aacgatcgcc ccgcgcaggc aacgtggcga acgaaggctc
     1681 gccgtcgcaa ggttcttttt ctcattacag agtcgatcac aagggattgc ctcaacggcc
     1741 gactgcagcg gacgaacggg cataagaagc cggatggttg tcatccggca acaaacgaaa
     1801 ggaggtaccg accaatgttt catcgttaca agatcaatcc gcacgagcga ggtctcctgt
     1861 tcaaggacgg cgatctcgtc accgtgctcg ggccgggtgc tcactggtac ctcgacccgc
     1921 tcatgaagct gcgtctgcac gtcgtgtcgc ctcgggtcaa tccgtggctg gtgcatccgt
     1981 cgctcgatct catcgcccgc tccggaatgc tcggcagcga agcgaccgtc atcgacctca
     2041 acgatcatca gcgcggtctg gtgtggatcg acaagcgttt cgcccgtctc ctggctccgg
     2101 gtctctacgt tctctggaac gtcgagcacg aggttcgcgt cgagatcgtc gacgcccgtt
     2161 ccatgcagct cgtccatgcc gatctcccgg tcatcatgaa gggctccggc gcaaacgagc
     2221 ttctggagat cgtcaacgtc gaggctggcc acaacggcgt ctggttccgc gacggtgcgt
     2281 accaggcgac gctcggtccg ggtgcgtggg tgttctggaa gggcaccgga cggctcaagg
     2341 tgatcgatgt cgatctccgc gagcagacgc tcgacatcag cggtcaggag atcatgaccg
     2401 ccgacaaggt cacgctgcgg ctcaatgctc tcgtcgtcta ccgcgtcacc gatccactgc
     2461 ggtcggttgc cgaggtcgat ggtgcgggac aggcgctgta tcgggccgcg cagctcgcgc
     2521 tgcgtgagat cgtcggtgcg agggagctcg acgtgctgct cgtcagcaag gaggcgctgg
     2581 ccaccgagct gtcggagctc acgaaggcac gtgccggcga gctgggtctg actgttcttt
     2641 ccaccggtat ccgtgacgtc atcctgccgg gcgacatgaa ggatctgatg aacaaggtca
     2701 tggaggcccg taaggccgcc gaggcgtcgc tcatcagccg gcgtgaggag acggcatcga
     2761 tgcgtatgca ggcgaacacg gcgaagatcc tcgagtccaa cccgacgctg atgaagctga
     2821 aggagctcga ggtcctcgag aaggtcgccg acaaggccaa cctcaccgtg cttctcggcg
     2881 gcgagagcgg cctgaacgag cgggtaatga agttgctgta aacacgtcag gggcggttcg
     2941 ccgcccctga cacttcagat cacggatgcg gcgtaatggc gatgatcgtc agcaccgcca
     3001 cacggttctt tccctccatc cggtcgggcc cgtacatgaa gaagacgcga taggctccag
     3061 gcgtctcgtt ctgcgcgtac gcttcgaaga cttcttcgcc ggagggacca tgcaatgagc
     3121 gaaacggatg ggtgttgagc gatggatggc gcaggttggt ctcgaggaga ccgagggtct
     3181 tgcgcacctg cttgagggtt ccgcggctcg ccgcgctctt ctggagatca gcgagattcc
     3241 ggcgagcctc aggtgtgaag tacagctcgc ggttcattca tcatcgtccg cgaactgcgc
     3301 aaacgaggcg ccacgctttg ccttcccgcc agctgcttcc tcgagcccgc gccgtacgga
     3361 gtcgatcgct ttctgattcc ggaacagcca cgcctcggaa gcaggaatcg atatgtgtgg
     3421 ctcgaggatg atccgcccct cgtcatcctt gtagacattg aagctggcat cgccgatgtc
     3481 gtccagcgca cgccccaacg cgacgcgctt acggctgtcg ggtcggacat ctcgtgcgag
     3541 aaggcggtat ccggaacggc gagcagcttt catcgccgca gaatagtcgc catgggccgc
     3601 gttgtaaagt gggcattacc cacccatcgc ttttgtatca aaggaaaagg gagggcatcg
     3661 agccctcccc tctccgactt gcgattgcgt tgcgtgctac ggcgtcgtaa cgatcacgtc
     3721 gtcgatgaac acgttgttgc cgaatgcgct gatgccaagg aagccgagtt ggacggtggt
     3781 tcccttgtag gcggtcaggt cgatcgtgtg ctgtttccag ccggtcgagc cgtcgtagcg
     3841 gttgatggcg gttccgacgc ttgcccacgt accggtcgag acctggacct gcacgcggtc
     3901 ggcggacgtc gagtagccgg tgtcgtggta catccagaac ttcagcgtga ctgtcgttgc
     3961 gctgctcgga atcgcgaagc cggtggtctg gtagacgcgc gtctgcgatc cgctggcgga
     4021 gtcgtaagag ttgaagagcg caaggtagct gccgccatgc gggctggctg tcggcagcgt
     4081 tcccgctgtc acgaaggtcc aggcgccggc ggtgccggag acctgcgcgg tggcccagcc
     4141 ggtgccggcg ctctcaaacc cgttggagaa gagcgtcgtc gggccggtgc agcttgcggt
     4201 gaagttggta ccggtgacgt tggcaccggc gatggtcacg gagctgctcg tgggagtgaa
     4261 ggtgcatccg ctcttgctcg gagtgacggt atacgtaccg ttggcgaggt tggcgatgct
     4321 gtagttgttc gatgcgtcgc ttgtcgcgcc ctgcgatccg gccgtgacgg tggcgcccgg
     4381 cgttccggcg ctgccggaga tcgagtaggt ggccaccggg gtcgtcacgg tgccggtgat
     4441 ggtgaagttg ccggcggtgt aaccgttcac gccgacccac caggtgccgg cagatgggtt
     4501 ggtcgccgag caggtctcgt taccggacga ggtgtagggc cggcaaacgt aggtggtcaa
     4561 tgtcggcttg gcgttgaact gcgtatagat gtcgacgtcc gaggtggcgc tggttgttgc
     4621 gaacgtgagg ttcgtggcgc cggccgggac ggtgatgtag tagtacttcc acgcgttcag
     4681 agccacactc tggcccgtca gcgcgacgcc gctggtgagc tgcgtgtcgc ccgagccgca
     4741 cgtcgcggtg aagttcgcga cggcgttcga ggacgtgatc gtcacggagc tcgacgcggg
     4801 cgagaacgtg cagcccgact tgctcggcgt aacggtgtag gtgccggcga cgaagcccgc
     4861 catcgtgtag ttgttcgatc catcgctcgt cgtcgatgag gtgcccgcgg tgacggtggc
     4921 gcccgaggtg ccgac
//
LOCUS       DATLVW010000392         7066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_551638, whole genome shotgun sequence.
ACCESSION   DATLVW010000392 DATLVW010000000
VERSION     DATLVW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551638"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VNN25_11820"
     CDS             <1..702
                     /locus_tag="VNN25_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXH92259.1"
                     /translation="WNHPQRERWVPGGGGMCLHTILLDPRSLDRIRIAVSTAGMYVTE
                     DGGETWRPSNKGVRADFLPDKYPEFGQCVHKVVQSKVNPDRMFLQNHWGLYRSDDSGE
                     KWKDVAKGVPSDFGFAMAIHPEDPDCAWIVPLESDGFRCTPEGKLRVYRTENAGKSWE
                     AMTKGLPQRDAYETILRDALANDPLDPAGVYFGTRNGKLFGSSDEGKKWSQLADGLPA
                     VVSVKAVSISLPRKR"
     gene            705..986
                     /locus_tag="VNN25_11825"
     CDS             705..986
                     /locus_tag="VNN25_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXH92260.1"
                     /translation="MSLVFVIPGPLRELAGNRAEVRVKGSAESASKALSLLWEQCPGV
                     RDRVVTEIGEVRPHVNIFIDGESIRFTGGLQSPVRDGAEIVILPALSGG"
     gene            complement(1065..1766)
                     /locus_tag="VNN25_11830"
     CDS             complement(1065..1766)
                     /locus_tag="VNN25_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXH92261.1"
                     /translation="MSEKFPSRPWFEVAEHVGKSTFRLYTDEAMGTGFLLMLGKGSDT
                     HVAVLATAWHVVKDAIGGTSLRICDRDGKEVVRSRKGNIAVMEIGNGLDSAIVVIQSP
                     NQIFHEGALLPLLPVAVQLKTGAEIAWMGYPGIVEPEICFFQGYISGTLKNPPVYLVD
                     GVAINGVSGGPVFDDRCHLVGLVSAYLPNRVGPETTLPGVSWVVPINFIYAWITAHLN
                     PRDLYADPNSVYNLS"
     gene            complement(1833..2183)
                     /locus_tag="VNN25_11835"
     CDS             complement(1833..2183)
                     /locus_tag="VNN25_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HXH92262.1"
                     /translation="MGLARSTTFISILAGLFAIGGASILYRFDPATVHFFPRCVFHEL
                     TGLQCPGCGTTRALHHLLHGDIAGAFRLNAMLFVVAPFGAIATVSRRFATHPITGWGA
                     VIVTVVWWVGRNVV"
     gene            complement(2152..2466)
                     /locus_tag="VNN25_11840"
     CDS             complement(2152..2466)
                     /locus_tag="VNN25_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HXH92263.1"
                     /translation="MSVCPNCGTGAPVFAATPQIQTPQIQNYLVPAILVTLCCCVPAG
                     IVAIVYAAQVNSKLAGGDIAGAQESARLAKIWSWVGFGCGALLGIIYAIAGGLSSLNN
                     VH"
     gene            complement(2505..3119)
                     /locus_tag="VNN25_11845"
     CDS             complement(2505..3119)
                     /locus_tag="VNN25_11845"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HXH92264.1"
                     /translation="MQYCAYCGNTVEAVSYAPCPRCGNPSNGAPRPVATASKGTNTAM
                     IVVIVIVGGLFVVAVIGILAAIAIPNLLTAQQRAKQKRTMADIRSLSAAVEAYSTDKN
                     EYPKGSTTSDLALALAPTYIKTVPAVDGWGHSLQYACLKDTTTPDSDKCAGYVIGSAG
                     KDLRFEHDSLLETVSAGERTTTNFDCDIVYSNGKFVEYPEGVQH"
     gene            3326..5071
                     /locus_tag="VNN25_11850"
     CDS             3326..5071
                     /locus_tag="VNN25_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92265.1"
                     /translation="MSPLRRLFHYFGLHKTALTLGALCVMGSAAFSLAKPLIVGNAVN
                     ALTKAVARGEMIRYGLMLIGAAALEGLFLFLHRRILIGVSRHIEYEMRNDFYDHLQSL
                     PVSYYQEQRTGDLISRATNDLSAVRMLMGPAVMHALSSLIVVIGAFVMMLRISREMAL
                     ISLTSVPVIVLMVQHFGQRIHVRFKSIQDYFGDISARVQENLSGVRVVRAFGRERAEA
                     ARFAEMNREYVERNRTLIKLTATFYPLLHAMIGVMFGVIFFIGSRKMIHGALTIGAFV
                     AFQLYLGRMVWPLIALGWVTNLFQRGMASMVRLHEVWSVAPEVLTTAGERVTAPVRGE
                     IEIRHLTFSYAGANRPTLRDINLHVQPGQTLGIVGRTGSGKSTLLALIMRTFEPPPGT
                     IYIDGRPIESFPPAQLRQWIGAVPQETFLFSESIAENIRFGRREATIDEVNESARLAG
                     LSSDVTTFPQGLETLIGERGITLSGGQKQRTAIARALVRDPAILILDDSLSAVDTNTE
                     EQILRALREIRKGRTALIVSHRVSSVKDADTIVVIDDGRIVERGTHDALLARDGYYAN
                     LYRRQTLEEEIEEIA"
     gene            5225..>7066
                     /locus_tag="VNN25_11855"
     CDS             5225..>7066
                     /locus_tag="VNN25_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92266.1"
                     /translation="MSDIERHEDEGAKAFDPHLARRLLRYLAPYRLRAVVSVGLVILS
                     SLLEIAGPAIVAVAIDLYVAPKQGAQVAGISRRIGAWLLAHGLALDPLSGINVAGALY
                     FLVLIAGFGVLYTQMVLMNLMGQYIMYDLRKQIFGHLQKLDIQFFDRNPVGRLMTRVT
                     TDVDALNDMFTAGFVSIFGDIFVLAGIVGMLFWMNWRMALVLFSIIPLIILASAWFRA
                     GARITYRKTRARIAAINAFLQEHISGMATVQLFNREADEAEKFEGLNARHRDANIEAI
                     FYYSVFYPVVELIETIGLALIVWYGGGQVIQGTLSIGVMVAFFQYAQRFYEPISDLSE
                     KYNILQAAMAASERVFKLLDTPPRILDDATIEVDHFESLEFRSVSFAYNEPDWVLQNV
                     SFTVRRGERVALVGHTGAGKTTVTALLLRFYEPQRGEILINGVDIRKYTIASLRSLFA
                     IVQQDFFLFTGNIAQNISLGNPDIGPERVREAAIRVQADRFIQWLPEGYEAEVRERGA
                     GLSVGEKQLLSFARALAFNPPVLVLDEATSSIDTETEVLIQEAIQTLMEGRTSIVIAH
                     RLSTIRSADTILVFHHGEIRERGTHDELIQHGGLYRKLYEIQYRETAA"
BASE COUNT         1313 a         2140 c         2223 g         1390 t
ORIGIN      
        1 tggaatcatc cgcagcgcga acgctgggtg ccgggcggcg gcgggatgtg cctgcacacg
       61 atcctcctcg atccgcgcag cctcgatcgc atccgcatcg cggtgtcgac ggccgggatg
      121 tatgtgaccg aggacggagg agagacgtgg cggccatcga acaaaggggt tcgcgccgac
      181 tttctgcccg acaagtatcc ggagttcggc cagtgcgttc acaaggtcgt tcaatcgaaa
      241 gtgaatcccg atcggatgtt cctgcagaat cactggggcc tctatcgcag cgacgacagc
      301 ggcgagaagt ggaaggacgt cgcgaagggc gttccgtcgg acttcggttt cgcgatggct
      361 attcaccccg aggatcccga ttgcgcctgg atcgtgccgc tggagtcgga tgggttccgc
      421 tgcacgccgg aagggaagct tcgcgtctat cgcacggaga atgccggcaa gagctgggag
      481 gcgatgacga aggggttgcc gcaaagggac gcctacgaaa cgatccttcg cgatgcgttg
      541 gccaacgatc cgctcgaccc ggccggtgtg tatttcggaa cgcgcaacgg aaagctcttc
      601 ggttcttcgg acgaggggaa gaagtggtcg cagctcgcag atgggctccc ggccgttgtc
      661 tcggtcaagg cggtctcgat ctcactgccg cggaaacgct gatcatgtcg ctcgtcttcg
      721 taatccccgg tccgctgcgt gagcttgctg gtaatcgcgc cgaggtgcgg gtcaagggct
      781 cggcggaatc ggcgtcgaag gcgctgtcgc ttttgtggga gcaatgtccg ggagtccgcg
      841 atcgcgtggt cacggagatc ggggaagtgc gcccgcacgt caacatcttc atcgacggcg
      901 agagcatccg gtttacggga gggctgcagt cgccagtgcg ggatggggcg gagatagtga
      961 ttcttccggc gttgagtggg gggtagcggg tttcgccgtt tggaagtcag caagccagaa
     1021 cgcgtttcat gcgccgagaa tcttgctaag catagaggca ttgattacga aagattgtac
     1081 acactgtttg gatcggcgta gagatctcta gggttcaaat gcgccgtgat ccaagcataa
     1141 atgaagttga tcggcactac ccacgagact ccgggcagcg ttgtttctgg cccaactctg
     1201 ttcggtaagt atgctgaaac taacccgacc agatgacaac gatcatcgaa aaccggaccg
     1261 ccgctcacac cgttgatcgc tacgccgtca acgaggtaca cgggtgggtt ctttagggtc
     1321 cctgagatgt aaccctgaaa gaagcagatc tcgggctcga caatgccagg atagcccatc
     1381 caagcgattt cagcccccgt cttgagctgc acagcgacgg ggagaagcgg cagtagggca
     1441 ccctcgtgaa agatttgatt cgggctctga atgacaacga ttgctgagtc aagtccgttg
     1501 ccgatttcca tcaccgcgat gttccctttg cgtgacctga cgacttcctt tccgtcccga
     1561 tcacagattc tcaaagaggt accgccaatt gcgtccttga caacgtgcca agcggttgca
     1621 agtacagcta cgtgagtatc ggaccctttt ccgagcatca gcagaaatcc cgttcccata
     1681 gcttcgtcgg tgtagagcct aaacgtggat ttgccgacat gctcagcgac ttcgaaccat
     1741 ggtcggctcg gaaacttttc ggacattggt gtaatcctta ttggttccag ttagattcag
     1801 accgcccgcc gacactcccg ggtttaggat cgctacacaa cattccgccc aacccaccac
     1861 acaaccgtca cgatgactgc gccccatccc gtgatcggat gcgtcgcaaa tcgccgcgac
     1921 accgttgcga tcgcgccgaa cggcgcaacc acgaagagca tggcgttgag gcggaaggcg
     1981 ccggcgatgt cgccgtgtag gagatggtgc agcgcgcgcg ttgtgccgca gccggggcat
     2041 tgcagtccgg tcagttcgtg gaagacgcag cgcgggaaga agtgcaccgt cgccggatcg
     2101 aagcgataga ggatcgaggc tcctccgatc gcaaataacc cggccaggat gctaatgaac
     2161 gttgttgagc gagctaagcc caccggcaat cgcatagatg attcccagga gcgcgccgca
     2221 gccgaaaccg acccagctcc agattttggc gaggcgcgcc gactcctgcg ctccggcgat
     2281 gtcgccgcct gcgagtttcg agttgacctg cgcggcgtag acgatggcca cgattcctgc
     2341 cggtacgcaa cagcagagcg tgaccaggat cgcgggcacg aggtagttct gaatctgcgg
     2401 agtctgaatc tgcggcgtcg cggcgaagac cggcgcgccg gtgccgcagt tgggacagac
     2461 gctcatgccg ttggggcggc tcgttccgca agctgtgcaa tacatcagtg ctgcactcct
     2521 tccgggtact ccacgaactt gccgttggag tagacgatgt cgcagtcgaa gttcgtcgtg
     2581 gtccgctctc cggccgaaac cgtttcgagc agtgagtcat gctcgaagcg gaggtctttc
     2641 ccagcactgc cgatcacgta tccggcgcac ttgtcgctgt ccggtgtcgt ggtgtccttc
     2701 aggcaggcgt actgcagcga gtgtccccac ccgtcgacag ccggaacggt tttgatgtac
     2761 gtcggggcga gcgccagggc gagatcggac gtggtcgagc ctttcgggta ttcgttcttg
     2821 tccgtggagt aggcctcgac ggcagccgac agcgagcgga tgtcggccat cgtgcgcttc
     2881 tgcttcgctc gttgctgcgc cgtaagcaga ttggggatgg cgatggcggc caggattccg
     2941 atgacggcga cgacaaacag accgccgacg atgacgatca cgacgatcat cgcggtgttc
     3001 gtcccttttg aagcggtggc aacgggacgc ggcgcgccgt tgctcgggtt tccgcatcgc
     3061 gggcatggag cgtaggaaac ggcttcgacg gtgttaccgc aataggcgca gtactgcatt
     3121 gcttccccct gtcgactcta ccggttgtgg attcaggtaa ttgtatccga gggggtgggg
     3181 agtggggggt agggggtggg agctgtgatc aatcacagag actcagagga cacagagatc
     3241 aaggtccgga ccccgaactc cgctgccact cccgtcccca caccctactc cccactcccc
     3301 acccccgcta gaatgcaaaa ctcgcatgtc cccccttcgc cgcctcttcc actacttcgg
     3361 ccttcacaaa acagcactga cgctgggggc gctttgcgtg atggggtcgg cggcgttctc
     3421 gctggcgaaa ccgctcatcg tcggcaacgc ggtgaacgcg ctgacgaagg cggtcgcgcg
     3481 cggggagatg attcgctacg ggctcatgct catcggcgcc gcggcactcg aagggctctt
     3541 cctatttctg catcgccgta tcctcatcgg tgtctcgcgg catatcgagt acgagatgcg
     3601 caacgacttc tacgatcacc tccagtcgtt gccggtgtcg tactaccagg agcagcgcac
     3661 cggcgacctg atctcgcgcg ccacgaacga tctttccgcc gtgcgcatgc tgatggggcc
     3721 ggccgtgatg cacgcgctct cgtcgctgat cgtcgtcatc ggcgcgttcg tgatgatgtt
     3781 gcgcatcagc cgcgaaatgg cgttgatctc tctgacgtcg gtcccggtga tcgtcctcat
     3841 ggtgcagcac ttcggtcagc ggattcacgt caggttcaaa tcgattcagg actacttcgg
     3901 cgacatctcg gcacgcgtgc aggagaacct ctccggcgtg cgcgtggtgc gcgcgttcgg
     3961 acgcgagcgc gcggaagcgg cgaggttcgc ggagatgaac cgcgagtacg tcgagcgcaa
     4021 tcgcacgctc atcaaactga cagcgacgtt ctacccgttg ctgcacgcga tgatcggggt
     4081 gatgttcggc gtcatcttct tcatcggcag ccgcaagatg attcacggtg cactgaccat
     4141 cggcgccttc gtcgccttcc agctctacct cgggcgcatg gtctggccgc tgatcgcgct
     4201 cggatgggtg acgaatctct tccagcgcgg gatggcgtcg atggtgcgtc tgcacgaggt
     4261 gtggtcggtg gcgccggagg tgttgacgac tgccggcgag cgcgtcacgg ctcctgtccg
     4321 cggcgagatc gagatccgcc atctgacgtt ttcctacgct ggcgcaaacc gtcccaccct
     4381 tcgcgacatc aacctccacg ttcaacctgg ccagaccctc ggcatcgtcg gccgcacggg
     4441 cagcggcaag tcgacgctgc tcgcgctgat catgcgcacg ttcgagccgc cgccgggaac
     4501 gatctatatc gacggccgtc cgatcgagtc attcccgcca gcacaactcc ggcaatggat
     4561 cggggccgtg ccgcaggaga cgttcctctt ctccgagtcg atcgcggaga acatccgttt
     4621 cggacgccgc gaggcaacga tcgacgaggt gaatgagtcc gcgcggctgg ccggactcag
     4681 ttccgacgtc acgacgttcc cgcaaggtct ggagacgctg atcggcgagc gcggcatcac
     4741 gctctccggc ggccagaagc agcgcacggc catcgcacgc gcgctcgtgc gagatccagc
     4801 catcctgatc ctcgacgatt cgttgagcgc ggtcgatacg aacaccgagg agcagatttt
     4861 gcgggctctg cgcgagatca ggaaagggag gacggcgctg atcgtctcgc accgcgtctc
     4921 ctcggtgaag gacgccgaca cgatcgtcgt catcgacgac ggccgcatcg tcgagcgcgg
     4981 tacacacgac gccctgctcg cacgcgacgg ctattacgcg aatctatatc gaagacagac
     5041 gttggaggag gagatcgagg aaattgctta attgcgcggc cctggcgggc cgcgcgttgt
     5101 cggttgtcgg ttgattgcgc ttcgcgcgtt gtcgatgcgt ctggcctgca tcggttttga
     5161 caacgcgcga agcgcaatca accgacaacc gacaaccgct agcacgcaaa gcgcgataaa
     5221 cacgatgagc gacatcgagc gacacgaaga cgagggtgcc aaggccttcg acccgcacct
     5281 tgcgcggagg ctgctgcgct atctggcgcc gtatcgcctg agggcggtgg tgtcggtggg
     5341 gctggtcatc ctttcttcgc tgctggagat tgccgggccg gccatcgtgg ccgtggcaat
     5401 cgatctctac gtcgcgccga agcagggagc gcaggttgcc gggatcagcc ggcgcatcgg
     5461 agcgtggctg cttgcgcatg gattggcgct cgatccgctc agcggtatca acgtcgccgg
     5521 cgctctctac ttcctggtgc tgattgccgg tttcggcgtg ctttacacgc agatggtgct
     5581 gatgaatctg atggggcagt acatcatgta cgacctccgc aaacagatct tcggccacct
     5641 gcagaagctg gacattcagt tcttcgaccg caatccagta ggccggctga tgacgcgcgt
     5701 gacgacggac gtcgacgcgc tcaacgacat gttcaccgca ggtttcgtct cgatcttcgg
     5761 cgacatcttc gttctggccg ggatcgtcgg catgctcttc tggatgaact ggcggatggc
     5821 gctggtgctt ttctccatca tccccctcat catcctggcc tcggcgtggt tccgtgctgg
     5881 cgcccgcatc acgtaccgca agacgcgcgc gcgcatcgcc gccatcaacg ccttcctgca
     5941 ggagcacatc tccggcatgg ccacggtgca gctcttcaac cgcgaagccg acgaggcgga
     6001 gaagttcgag ggactcaacg cgcggcatcg cgacgccaac atcgaggcga ttttctatta
     6061 ctcggtcttc tacccggtcg tggagctcat cgagactatc ggcctcgcac tcatcgtctg
     6121 gtacggcggc ggtcaggtga ttcagggaac gctttcgatc ggcgtgatgg tggcgttctt
     6181 tcaatacgcc cagcggttct acgagccgat ctccgacctc tccgaaaagt acaacatcct
     6241 gcaggcggcg atggcggcga gcgagcgcgt gttcaagctg ctcgacacgc cgccccgcat
     6301 tctcgacgac gccacgatcg aggtcgatca cttcgagtcg ctcgagttcc gcagtgtctc
     6361 gttcgcctac aacgagccgg attgggtgct gcaaaacgtc tcgttcacgg tcaggcgcgg
     6421 cgaacgtgtc gcgctcgtcg gccacactgg cgcgggaaag acgacggtga ccgcgctgct
     6481 ccttcgtttc tacgaaccgc agcgcggtga gatcctcatc aacggcgtcg acatccgcaa
     6541 gtacacgatc gcgtcgttgc gctctctttt cgcaatcgtc cagcaggact tcttcctctt
     6601 caccggcaac atcgcccaga acatctccct cggcaatccc gacatcggtc cggagagagt
     6661 gcgcgaggca gcgattcgcg tgcaggcgga ccgcttcatc cagtggcttc cggaaggcta
     6721 tgaagccgag gtacgggagc gcggcgcggg gttatcggtg ggggagaagc aactcctctc
     6781 cttcgctcgc gcgctggcgt tcaatccgcc ggtgctcgtg ctggacgagg ccacttcatc
     6841 gatcgatacg gagaccgagg tgctgattca ggaggcgatc cagacgctga tggaggggcg
     6901 gacatcgatc gtcatcgccc accgcctctc cacgatccgc tccgccgata cgatcctcgt
     6961 cttccaccac ggcgagatcc gtgagcgcgg cacgcacgac gagctgatcc agcacggagg
     7021 gctgtaccgg aagctgtacg agatccagta tcgggagacg gcggcg
//
LOCUS       DATLVW010000393         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_143469, whole genome shotgun sequence.
ACCESSION   DATLVW010000393 DATLVW010000000
VERSION     DATLVW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143469"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..>2109)
                     /locus_tag="VNN25_11860"
     CDS             complement(10..>2109)
                     /locus_tag="VNN25_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92267.1"
                     /translation="GRTIQPSDDAGGGRVVMLSDTLWRRRFASDPRIVGATVAISGEP
                     FEIIGVAPASFSGPRGWLVGTQFWIPLGTESRLKESPRTAAPRATARDDRRLTVFGRL
                     ATEVTIAAASAEMQTVAERLDESFPPRFRGKGVGATDRPWRAKTMAAISDDEDSAVRR
                     FGMTLVALVGLVLVVACTNLGNLVLARGSTRLRELAVRHALGASRWRLVREQCLESVM
                     LAAGGAFASYVMFELLRVVMDTEFSLAMPFGGRTTLSFHPALNVPALTIAVSSLLIAL
                     VVFGLEPAIQLTRTLDVRGALAGGSAAGPQRGRRQRLLLRWQVAIAAGFFIIATMFVK
                     YSIAEARHDSGIDLDRIGVAVLNFDTPEWNEPRARRIFDRVLDELRRHPSVEAASLSS
                     GLPFGVPSTLRLSISPLEQTSAARMDPQVVTTIAGVPSMFRTLGVEILRGRGFDDRDG
                     PAAPRVVVLSEFTARQMFGTADVVGREMAVGQTRSPTATIVGVAADTDVGSIFADPRP
                     LAYVPLTQHYQPLITVVARASGSVTNAVGAMREAIRRADPDVAVDTIGTGRSVLAGPF
                     QVLRGLGMTAVGLGAITLLLAMAGLYGIQSHIIANRTREIGVRMSLGATAGQIKRMVL
                     VDGYRPVLDGLIFGLSIGLVGRAIARAYLELDVAIVDPWMLVVTPIPLICAAFFACYL
                     PARRAAAVDPNVALRSE"
BASE COUNT          377 a          740 c          706 g          286 t
ORIGIN      
        1 ccggcgtggc tattcgctgc ggagcgctac gttgggatcg accgccgcgg ccctgcgggc
       61 cggcaagtag caggcaaaga acgcagcaca gatcaacgga atcggcgtca ccaccagcat
      121 ccacggatcg acgatggcga cgtccagctc gagatacgcg cgcgcgatcg cccggccgac
      181 gaggccaatc gacaacccga agatgagccc gtcgaggaca ggacgatagc cgtccacgag
      241 caccatgcgc ttgatctgtc cggcggtggc ccccaacgac atccgcacgc cgatctcccg
      301 cgtgcggttt gcaatgatat gcgactggat gccgtagagg cccgccatgg cgagaagcag
      361 cgtgatcgcg ccgaggccga ccgccgtcat gccgagcccg cgcaggacct gaaacggtcc
      421 ggcgagcacc gatcgtcccg tcccgatggt gtcgaccgcc acgtccggat cggcacgccg
      481 gatggcctcg cgcatcgcgc ccaccgcatt cgtcaccgat ccggatgccc gcgcgacgac
      541 cgtgatgagc ggctggtagt gctgcgtcag cggcacgtag gccagtggcc gcggatccgc
      601 gaagatactg cccacgtccg tatcggcggc aacgccgacg atcgtggccg tcggggaccg
      661 cgtctggccg accgccatct cccgcccgac gacgtcggcg gtaccgaaca tctgccgtgc
      721 cgtgaattcg ctcaggacca cgacgcgtgg cgcggccggc ccgtcgcgat cgtcgaaacc
      781 gcgtccacga aggatctcca cccccagcgt tcggaacatc gaggggaccc cggcgatcgt
      841 cgtcaccacc tgcgggtcca ttcgcgcggc gctcgtctgt tccagtggtg agatggacag
      901 acgcagcgta ctgggcacgc cgaagggcaa tccactcgac agtgaggccg cctcgaccga
      961 cggatggcgc cgcagctcgt cgagtacccg gtcgaagatg cgccgtgcgc gaggctcgtt
     1021 ccactccggc gtgtcgaaat tgagaacggc gacgccgatt cgatccaggt cgatgccgga
     1081 atcatggcgc gcctccgcga tcgagtactt cacgaacatg gtggcgatga tgaagaaccc
     1141 ggccgcgatg gcgacctgcc accgcaggag aagccgctgg cgccggccgc gctgcggtcc
     1201 ggcggccgat ccgccggcga gcgcgccgcg aacgtcgagc gtgcgcgtca gctgaatcgc
     1261 cggttcgagg ccgaagacga cgagcgcgat gaggagcgac gagaccgcga tcgtcagcgc
     1321 cggcacgttc agcgcgggat gaaacgacag cgtggtacgg ccgccgaaag gcatggcgag
     1381 gctgaactcg gtgtccatca cgacgcgcaa cagctcgaac atgacgtagg acgcgaatgc
     1441 gccgccggcc gccagcatca cgctctcgag acactgttcg cgcacgagcc gccacctcga
     1501 tgcgccgagg gcgtgccgca cggcgagctc gcgcagccgt gtacttccgc gggcgagcac
     1561 gaggttgccg aggttcgtgc aggcgacgac cagcaccaac ccgacgagcg ccacgagcgt
     1621 catcccgaac cggcgcacgg cggagtcctc gtcgtcggag atcgccgcca tggtctttgc
     1681 gcgccacggg cgatccgtgg cgccgacgcc cttgccacgg aatcgcggcg gaaaagactc
     1741 gtcgagccgc tcggcgacgg tctgcatttc cgccgatgcc gcggcgatcg tcacctccgt
     1801 tgcgaggcgg ccaaagacgg tgagccgtcg atcgtctcgt gctgtggcgc gcggcgccgc
     1861 cgtccggggg gactccttca accgtgactc ggtaccgagt ggaatccaga actgcgtgcc
     1921 caccagccat ccgcgaggac cgctgaacga ggccggcgcc acgccgataa tctcgaaggg
     1981 ttctccggag atcgccacgg tggcgccgac gatccgcgga tcggacgcga accgccgccg
     2041 ccacagcgta tcgctcagca tcaccacacg tccgccgccg gcatcgtcgc tcggctgaat
     2101 cgtgcgtcc
//
LOCUS       DATLVW010000394         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_350899, whole genome shotgun sequence.
ACCESSION   DATLVW010000394 DATLVW010000000
VERSION     DATLVW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350899"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VNN25_11865"
     CDS             complement(<1..602)
                     /locus_tag="VNN25_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXH92268.1"
                     /translation="MKKIIGSLAVLAWALSLTAQTSAKTSGQTPASATQTPAAPPAQP
                     FPPITQLPTGATQLEPNAGSTSFTFVVAGDNRPAHSGDPLTTAMLDMVTRLSANPPDF
                     VVWNGDTVFGKQEVGIAEQYSQFLGALKPLTVPVFNAPGNHEMVVQTLVACGTASDPW
                     NAELPDFSASMMADYETSMGPPYGMFRYGNAAFVIINTDDV"
     gene            716..791
                     /locus_tag="VNN25_11870"
     tRNA            716..791
                     /locus_tag="VNN25_11870"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Ala,seq:cgc)
     gene            complement(799..1833)
                     /locus_tag="VNN25_11875"
     CDS             complement(799..1833)
                     /locus_tag="VNN25_11875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH92269.1"
                     /translation="MNRPSTKHHVAVIGGGVSGLTTAIVLTERGHHVELFAGSVGMAT
                     TSGKAAAIWFPHHIGDPNDEVSKSAVKEWAETTYDRLEGLIPENGRPDASIGISMVEF
                     RVVSRNELPESERPWAPSRDLKPEDLPQGRCFGYGIDVPLIETPVYLRYLERRLESKV
                     GEECIHRGVKIGGISAIDDRFSIVVNCAGIDGPDFSRMEDPTAMNPGRGVVILGCTAK
                     PYAILDADEYKSGKLMYIVPRRLSGRCVIGGSDTNDNTPAPDAKEIAEIVERCSAADS
                     TLEVEVSSAAVGLRPVRPKGVRLEREDIDGRAVIHNYGHGGSGFTVSWGCAEEVARIV
                     AAIRAPGTNV"
     gene            1994..>2655
                     /locus_tag="VNN25_11880"
     CDS             1994..>2655
                     /locus_tag="VNN25_11880"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HXH92270.1"
                     /translation="MHSCPRTGSISPDPPESITTVGGALCILKWLEEHFFDCDMWPGH
                     TFPFGLWFRGHWQPGLKLEPGAFRGELAIDAALRKDPKTPEIEKGTWDETNVYEHLRL
                     RVPLHQRLYQSAFDWLCLMEHYLLPTRLLDWSESILPALFFATRKRHDIPGELIVLNA
                     KRLNNRSKKHPTISTPTDGHVVIRAEMATTRSLTGLRLKSTVRAALAEEGLLQANGGL
                     PR"
BASE COUNT          528 a          858 c          738 g          531 t
ORIGIN      
        1 acatcgtcgg tattgatgat gacgaacgcg gcgttgccgt agcggaacat cccatacggc
       61 ggtcccatcg aggtctcgta atcagccatc atcgaggcgc tgaagtcagg tagctcggcg
      121 ttccacggat cgctggccgt accgcaggca accaacgtct gcacgaccat ctcgtgattg
      181 cccggtgcat tgaataccgg cacggtgagc ggcttgagcg cgccgaggaa ctgcgagtac
      241 tgctcggcga tgccgacctc ctgcttcccg aaaaccgtat cgccattcca gacgacaaag
      301 tccggcggat tggccgaaag ccgcgtcacc atatcgagca tcgccgtcgt caacggatcc
      361 ccgctatgcg ccggccggtt gtcgcccgcg acgacaaacg tgaagctggt cgaccccgca
      421 ttcggctcga gctgcgtcgc cccggtcggc aactgggtga tcgggggaaa gggttgggcg
      481 gggggcgccg caggcgtctg cgtggcagac gcaggcgttt gaccggacgt cttcgcggaa
      541 gtctgcgcgg tcagggagag agcccaggcg agcacagcga gcgacccaat gattttcttc
      601 atgcgccaag tttcgcacaa aaccggttgc cccgatccgg acccgcccct ataatccgcg
      661 cgccggaatt ctcctccggc aagctgccgt tagcagcgat actttccacc atcgtggggc
      721 tgtagctcag ctgggagagc gcctgcttcg cacgcaggag gtcatcggtt cgatcccgtt
      781 cagctccacc aatcaaactt agacgttggt tccaggcgcc ctgatcgctg ccacgattct
      841 cgcgacctct tcggcgcatc cccaagagac tgtaaagccg ctgccgccgt gtccgtagtt
      901 atgaatcaca gcacggccat cgatatcctc tcgttccaac cggactccct tcggtcgcac
      961 gggacgtagt cccacggcgg cgctggagac ctcgacctcc agtgtggaat cagccgcgga
     1021 gcaccgttcg acgatttccg cgatttcctt cgcgtccggc gccggcgtgt tgtcgttcgt
     1081 atccgacccg ccaatcacgc atcggccgct cagacgccgg gggacgatgt acatcaactt
     1141 accgcttttg tactcgtcgg cgtcgagaat ggcgtagggc tttgccgtgc agccaaggat
     1201 cacgacgcca cggcccggat tcatcgccgt cggatcctcc atcctcgaaa agtcgggccc
     1261 gtcgatcccg gcgcaattca cgacgattga aaacctgtca tcaatcgcgg agatcccccc
     1321 gatcttcact cctcgatgaa tgcactcctc gccaactttg gactcgagcc gtcgctcgag
     1381 ataccgaaga tagacgggcg tctcgatgag cgggacgtcg atgccgtacc cgaaacaacg
     1441 cccctgcggc agatcctccg gtttcaaatc cctggacggc gcccagggcc gttcgctctc
     1501 cggcagctcg tttctgctga cgactcgaaa ctcgaccatc gagataccga tgctcgcgtc
     1561 cgggcgtccg ttctctggaa tgaggccttc taggcgatcg tacgtcgtct ccgcccattc
     1621 cttcacggcg gatttcgaca cctcgtcatt cggatcaccg atatggtgcg ggaaccagat
     1681 ggccgccgct tttcccgacg tcgtagccat gcctacactt cctgcgaaga gctcgacgtg
     1741 atgccctcgc tcggtaagaa cgatcgcggt tgtgaggccc gacactccgc cgccaatgac
     1801 cgcaacgtga tgtttcgtgg aagggcggtt catctcctca tattagagga tttgttgctc
     1861 tcgctcgaat aacttatcga ctttcaaacc gatcgagctt gagcgaccga tcacgacacg
     1921 gagagcagcg ggttttcaat acagtgcctc caatccccaa tgctcagctt gcttgtgcta
     1981 ttattccttc ctgatgcaca gctgcccccg aacaggatcg atcagtcccg acccacccga
     2041 atccatcaca accgtcggtg gcgcgctgtg catactgaaa tggctggaag aacatttctt
     2101 cgactgtgac atgtggcccg ggcacacatt ccctttcggg ctgtggtttc gtggtcactg
     2161 gcaacccgga ctgaaactgg agcccggcgc gtttcggggc gagctcgcaa tcgacgccgc
     2221 cttgcgaaaa gaccctaaga ctcccgagat cgaaaaggga acgtgggacg aaacgaacgt
     2281 ctacgagcac ctgcggctga gagtacccct tcatcagcgc ctctatcagt ccgccttcga
     2341 ctggctatgt ctgatggaac attacctctt gccgacgcgc cttttggatt ggtccgagag
     2401 catcctgcca gccctcttct ttgccacaag gaaacggcat gacatccccg gcgagctcat
     2461 cgtcctgaac gcgaaacgac tcaataacag gagcaagaaa caccccacga tctcaacacc
     2521 gaccgacggg catgtcgtca ttcgagccga gatggcaacg acgcggtctc tgaccgggtt
     2581 acgcctcaaa tcaaccgtgc gcgctgcgct ggctgaggaa ggtcttctgc aggccaatgg
     2641 cgggttaccc cgaaa
//
LOCUS       DATLVW010000395         7375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_427137, whole genome shotgun sequence.
ACCESSION   DATLVW010000395 DATLVW010000000
VERSION     DATLVW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427137"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1358)
                     /gene="cls"
                     /locus_tag="VNN25_11885"
     CDS             complement(42..1358)
                     /gene="cls"
                     /locus_tag="VNN25_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HXH92271.1"
                     /translation="MIKRKWIEKTKRGSHVIRVPLIVFAALLLAVIGLTIMLWSAERG
                     TDPRLNVKNDGDFSTLLPSIVGLTQSNLEPGNHMQLLENGDQFFPLLLRDIAGAKESV
                     HVESYIWWKGAICEQIANALASKARQGVEVRLLIDASGGHKMDKDLEKLMLDAGVRFV
                     RFHQLSLANLGRMNNRDHRKEAIIDGRIGYIGGYGFAEQWTGHAQDKDHWRDAGLRVE
                     GPIVGRLQAAFAENWIEETGEIMAGEKYFPHLAAAGSTKAHLAYASPTGGVSSVQVLY
                     YLAIKAAHHEIIIQNPYMLPDDESVAAFGEAVKRGVDVRIMVPATTSTDSPVVQHASH
                     HLFGRLLKAGVKIFEYKKTLLHQKVIIVDGIWSCIGSTNFDRRALQLNDEVSMGVLDP
                     ALATQLKAVFENDMRFAEQRHFDEWDHRGIWHKAEDGLAYLASSQF"
     gene            complement(1355..2272)
                     /locus_tag="VNN25_11890"
     CDS             complement(1355..2272)
                     /locus_tag="VNN25_11890"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXH92272.1"
                     /translation="MAALANDAPVRRPILGILSILLLFILYRIFAVYTVRSGECRPKP
                     VDPNVRILTRDEAGRLVSFPERIGFPQQQQPLVVMTYNIEGHDELYDTDHAAKIAATI
                     KEFKPDIVGLQEVHDHTWQVRFRNQFEEIRKLTGMNGYFGRSYRSLGGDFGNAILTRG
                     EIVSAVVHPLPSVGEPRSVIESVIRIDGATINVYVAHLTAWARFNSVSRGVQLECLAK
                     HVRTSRFPYLLLGDFNAPPETAEVTKFEKEDAAQLCGPEIKLTHPFLGQRIDYIFADW
                     GWRVASTRAPATGASDHYPVITELYWSRN"
     gene            complement(2550..3290)
                     /gene="nagB"
                     /locus_tag="VNN25_11895"
     CDS             complement(2550..3290)
                     /gene="nagB"
                     /locus_tag="VNN25_11895"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HXH92273.1"
                     /translation="MTVFRRLKKTESNMNWETLTDYDALSDYAAKILLAAIRSDPRIV
                     LGLPTGRTPVRMYERVVGACSREYYCFRDAVTFNLDEYVGIEPSHPGSYCTFMKRHLF
                     DFIDVDPANAHIPNGVAPDLSAECSRYEKAIANAGGLGLTFLGLGANGHIAFNEPGTA
                     FDSRTRVVALTESTRVANESLFPEGNVPAQAITMGIATILASKSIVLLASGPRKRGAI
                     ERLRSGEISEQFPASALWQHPDVTVLVA"
     gene            complement(3287..4426)
                     /gene="nagA"
                     /locus_tag="VNN25_11900"
     CDS             complement(3287..4426)
                     /gene="nagA"
                     /locus_tag="VNN25_11900"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HXH92274.1"
                     /translation="MSHRSIFGRAFVAGELRDGVLITIADQQIIDVRDAVHAPSGSYA
                     ADGVIVPGFVDVHVHGGAGADFMDASADAVAAVTRFHAGEGTTSLAATTLSASREHLH
                     QAVAAIARASRAGAFGAQICGIHLEGPYISIARAGAQDVASIRPADIVELSALLAEAP
                     HLRWMMTVAPEIDGVRALIEHFRDRILFSIGHTAADYAGCVAALEWGASHFTHLFNAM
                     TGLDHRRPGAVGAALTSVEATAELIADGVHVHPAVLRMAALTMPHRVALITDAVRACG
                     MPDGKYKLYEHDITVADGAARLNDGTLAGSVLTMRQAVQNMVELAGLPLELVIPLAAE
                     VPARILGVADRKGKIAPGYDADLVVLTERFEVSATYLRGEILLSS"
     gene            complement(4434..5684)
                     /locus_tag="VNN25_11905"
     CDS             complement(4434..5684)
                     /locus_tag="VNN25_11905"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HXH92275.1"
                     /translation="MNRVERLIKIAGKKARVVIGLMSGTSVDAIDAVLVRIDGNARDG
                     ARIEKLRFASFEFPEEIQHRIKLLFERERDRKLTDPDTGEHPALWTIEEICHLDFVLG
                     ELFASAANRLIADAGLTNDDIDLIAAAGQTIWHRPRPAAEPSTGSLPWLDQPVTTRST
                     LAIGQAAVIAERTGIITIGDLRVRDVAAGGHGAPLVAYFDWGQLRHPKLARAMQNIGG
                     IANVTYIPPGAALDEVIAFDTGPGNMVIDSLMYIVSNGNETFDRDGAAAARGHVREDI
                     LAWCMSDPYFQLAPPKTTGRERFGRQFAARMAARFDGVEADDLIATATAFTAESIAHA
                     YREFIGRPVDEMIVAGGGARNPTLLRMLRERLPGTDVRIYEFLQEKEAMAMALIASDT
                     ISGLDTNVASLTGGHATVLGKICL"
     gene            complement(5681..6553)
                     /gene="murQ"
                     /locus_tag="VNN25_11910"
     CDS             complement(5681..6553)
                     /gene="murQ"
                     /locus_tag="VNN25_11910"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HXH92276.1"
                     /translation="MSQERLEASLDIDRRSAREIVQIIQEQDALVAAAVAAEGDRIAH
                     AVDQIADRLSHGGRLFYVGAGTSGRIAMLDASELPPTYGIDASLVSVIMAGGERAFLH
                     AVEGAEDDEDAAIEAVNAAVTAEDAVVGIAASGTTPYTVAAIRRANMLGALTVGITSN
                     PSSPLASEADIAIAPQTGPEVIMGSTRMKSGTTQKLVLNTLSTGVMILLGRVHSNLMI
                     DMPATNEKLRSRAVRMVELAAGVNRPAAVQAMRDAGGNVKLASVMAGKRLSLEEAKAI
                     LAANGGNLRKVLES"
     gene            6635..>7375
                     /locus_tag="VNN25_11915"
     CDS             6635..>7375
                     /locus_tag="VNN25_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXH92277.1"
                     /translation="METAVAGTREHRLAGDAFVGTPRLFRQHAGFSFGHWLATPVGDD
                     VREHLHVAAHFMFIAGGRFVTAAAGADRASPLIYNPPNTVHRDRFESSSGRFFAVSAD
                     SAAFCDIEPPPGVAHRIENVGLHAIVIRLMHECEWWSDDSPAIAESLCVALFAGMNRR
                     GRFVAPRWLGTATERLCEEYETPVAIADLARDLGVHRTHLTRTFLRAHGCTPAEFARL
                     HRIRRAATMLASTRTPIAEIALASGFSDQ"
BASE COUNT         1345 a         2337 c         2310 g         1383 t
ORIGIN      
        1 ctctcaaaac taattgcccg ctcctaatcc accgctaccc gctaaaactg cgagctcgcc
       61 agatacgcca ggccatcctc ggccttgtgc cagattccgc gatggtccca ctcgtcgaag
      121 tgccgctgct cggcgaaccg catgtcgttt tcgaagaccg ccttcagttg cgttgccagc
      181 gcgggatcga ggacgcccat gctcacttcg tcgttgagct gcagggcgcg gcggtcgaaa
      241 ttcgtcgatc cgatacacga ccagatgccg tcgacgatga tcactttctg atgcagcagc
      301 gtcttcttgt attcgaagat cttgacgccg gctttcagca ggcgcccgaa gaggtgatgg
      361 ctggcgtgct ggacgactgg gctatccgtc gatgtcgtcg ccggcaccat gatgcggaca
      421 tcgacgccgc gcttgactgc ttcgccgaag gccgccaccg attcgtcgtc cggcagcatg
      481 tagggattct ggatgatgat ctcgtggtgg gcggctttga tggcgaggta gtacagcacc
      541 tgcaccgatg agacgccacc ggttggcgac gcgtacgcca gatgcgcttt cgttgagccg
      601 gcggcggcaa ggtgtggaaa atacttctcg cccgccatga tctcgccggt ctcctcgatc
      661 cagttctcgg cgaaggccgc ctgaagccgg cccacgatcg gaccttcgac gcgaagcccg
      721 gcgtcgcgcc agtgatcctt atcctgcgca tgtccggtcc actgctcggc gaatccgtag
      781 ccgccgatgt agccgatccg gccatcgatg atcgcttcct tgcgatggtc gcggttattc
      841 atgcgcccga ggttggcgag ggaaagctga tgaaagcgaa cgaagcggac gccggcatcg
      901 agcatcagct tctccagatc cttgtccatt ttgtggccgc ccgaggcatc gatgagcaag
      961 cggacttcga cgccctgccg cgccttcgac gccagcgcgt tggcgatctg ctcgcagatc
     1021 gctcccttcc accagatgta cgactcgaca tggacgctct cttttgcccc ggcgatgtcg
     1081 cgcagcagca gcggaaagaa ctgatcgccg ttctcgagca gttgcatgtg attgccgggc
     1141 tcgagattcg attgtgtaag gccgacgatc gatggcagga gcgtagaaaa gtcgccatcg
     1201 ttcttgacgt tcaggcgcgg gtccgtcccc cgctccgcgg accagagcat gatcgtcagc
     1261 ccgatcacgg cgaggaggag cgccgcgaac acgatgagcg gcacgcgaat gacgtgcgaa
     1321 ccccgcttgg ttttttcgat ccactttctc ttgatcaatt cctgctccag taaagctcgg
     1381 tgatgacggg gtagtgatcg gacgcgccgg tagcaggagc gcgcgtactg gcgacgcgcc
     1441 aaccccagtc ggcaaagatg tagtcgatcc gctgcccaag gaagggatgg gtgagcttga
     1501 tctccggccc gcaaagttgg gcggcgtctt ccttctcgaa cttcgtgact tccgcggtct
     1561 caggcggagc gttgaaatcg ccgagcaaca ggtacgggaa ccggctcgtg cggacatgct
     1621 tcgccagaca ctcgagctgc actcctcggg acaccgaatt gaaccgcgcc cacgcagtaa
     1681 ggtgcgctac atacacgttg atcgttgccc cgtcgatgcg gatcaccgac tcgatcacac
     1741 tgcgtggctc tccgacgctc ggcagtggat gaacgaccgc ggagacgatc tcgcctcggg
     1801 tcagaatcgc attgccgaaa tcgccgccga gactgcggta gctgcggccg aagtagccgt
     1861 tcatgcccgt cagcttccgg atctcctcga actgattgcg aaaacgcacc tgccaggtgt
     1921 gatcgtgcac ttcctgcagt ccgacgatgt caggcttgaa ttccttgatc gtggcggcga
     1981 tcttcgcggc atggtcggtg tcgtacagct catcgtgccc ttcgatgttg taggtcatga
     2041 cgacgagcgg ttgttgctgc tgcggaaagc caatccgctc ggggaaggag acaagccgcc
     2101 ctgcctcgtc gcgcgtgagg atgcggacgt tgggatcgac cggcttcggc cggcactccc
     2161 cggagcgaac cgtgtagacc gcgaagatcc tgtagagaat gaagaggagc aggatcgaga
     2221 gaatcccgag aatcggtcgc cggacaggtg catcgttagc cagtgcagcc attgatgcgg
     2281 acgcaagagc gagaacaatg ccgggttgga ggaaagcgcg tgaccgggga tcgtacgaag
     2341 tgcactttag tgctcatgaa gagcgctccc tctacgtttg ctgggaactg ttccatgttc
     2401 catgagcgcg ccaatgcgcg tttccttacg gaacatatgg tccgaaagcc cctgaaatgg
     2461 ccggttgtcg tgcatgccaa tattgatgac gtctggagag catgtccaac accctgggga
     2521 ggaggtgtgt tgcggagcgt tcccgagacc tacgcgacca aaaccgtaac gtctggatgc
     2581 tgccacaacg cgctcgccgg gaactgctcg ctgatctctc ctgagcgcag acgttcgatc
     2641 gctcctcgct tcctcgggcc gctggccagc agcacgatcg acttggacgc gagaatcgtg
     2701 gcgatgccca tcgtgatggc ctgcgccggc acgttgccct cggggaagag agactcgttg
     2761 gcaacccgcg ttgattccgt aagcgccacg accctggttc gcgagtcgaa cgctgttccc
     2821 ggctcgttga aagcgatgtg gccgttggcg ccgaggccga gaaaggtgag gccgaggcct
     2881 ccggcattag cgatcgcttt ctcgtagcgc gaacactcgg cgctcaggtc cggcgccaca
     2941 ccgttcggga tgtgcgcgtt ggccggatcg acgtcgatga agtcgaaaag gtggcgcttc
     3001 atgaaggtgc aatagctgcc gggatggctc ggctcgatcc cgacgtactc gtcgagattg
     3061 aacgtcacgg catcgcggaa gcagtagtac tcgcggctgc atgcgccgac aacgcgctcg
     3121 tacatgcgga ccggcgtgcg gccggtcggc agcccgagga cgatgcgcgg atcggagcgg
     3181 atggcggcga gaagaatctt cgcggcgtag tcggaaaggg cgtcgtagtc ggtgagcgtt
     3241 tcccagttca tgttggattc ggtcttcttg agccgccgaa agacggtcac gaagacagca
     3301 gaatctcccc tcgcaagtac gttgccgaca cctcgaaacg ctcggtgagc acgaccagat
     3361 cggcgtcgta gccgggtgca atctttccct tgcggtcggc aactcccaga attcgcgcag
     3421 ggacttcggc cgccagcgga atcacgagct ccagcggcaa gccggccagc tccaccatgt
     3481 tctgcaccgc ctgacgcatc gtgagaacgc tgcccgcgag ggtcccgtcg ttcagtcgtg
     3541 ccgcgccgtc agcgaccgtg atgtcgtgct cgtaaagctt gtacttcccg tccggcatcc
     3601 cgcacgcgcg tacggcatcg gtgatcaatg cgacgcgatg cggcatcgtc agcgcggcca
     3661 tccgcagaac cgcagggtgg acatgcacgc cgtcagcgat cagctctgcc gtcgcctcaa
     3721 cggatgtgag tgccgccccg accgcacctg gacggcgatg atcgaggccg gtcatcgcat
     3781 tgaagagatg cgtgaaatgc gacgcgcccc attcgagcgc ggcaacacag ccggcatagt
     3841 cggccgcggt gtggccgatc gagaagagga tacggtcgcg gaagtgctcg atcagcgcgc
     3901 ggacgccgtc aatctccggc gccacggtca tcatccaccg caaatgcggc gcctcggcca
     3961 acaacgcaga gagctccacg atatcggcgg gccggatcga tgcaacgtcc tgcgctcctg
     4021 cgcgcgcgat gctgatgtac ggaccctcga gatgaatgcc gcagatctgc gcgccaaagg
     4081 cgcctgcgcg tgatgcgcgc gcaatcgccg ccaccgcctg gtgcaggtgc tcgcgtgatg
     4141 ccgaaagcgt cgtcgccgcc agcgacgtcg tcccctcgcc ggcgtgaaaa cgtgtcacgg
     4201 ccgctacagc gtccgcggac gcgtccatga agtcggcccc ggcaccgcca tgcacgtgga
     4261 cgtcgacgaa cccgggaacg atgactccgt ccgctgcgta actgccggac ggtgcgtgta
     4321 cggcgtcgcg aacatcgatg atctgctggt cggcgatggt gatcaacacg ccatcgcgca
     4381 gctcaccggc cacaaaggca cgtccgaaaa tactacgatg actcatagac gactcataag
     4441 cagattttgc cgagaaccgt ggcgtgcccg cccgtgagag aggcgacgtt cgtgtcgagt
     4501 ccggaaatcg tatcgctggc gatcaaggcc atggccatcg cttctttttc ctggaggaac
     4561 tcgtagatcc tgacgtcggt tcccggcagc cgctcgcgca gcatgcgaag cagcgtcgga
     4621 ttccttgcac caccacccgc cacgatcatc tcatccaccg gcctgccgat gaactcccga
     4681 tacgcgtgcg caatgctctc tgccgtgaac gccgtggcgg tggcgatcag atcatccgcc
     4741 tcgacgccgt caaaacgggc ggccatgcgc gccgcaaatt gtcttccgaa gcgctcccgc
     4801 cctgtcgtct tcggcggcgc gagctggaaa taggggtcgg acatgcacca cgccagaatg
     4861 tcctcgcgga catggccgcg cgccgcggca gcgccgtcgc gatcgaatgt ctcgttcccg
     4921 ttggacacga tgtacatgag cgaatcgatg accatgttgc cgggaccggt atcgaacgca
     4981 atcacctcat cgagcgccgc gccaggcgga atgtaggtga cgttcgcgat cccgccgatg
     5041 ttctgcatgg cccgtgcgag ctttggatgg cgcaactggc cccaatcgaa ataggcgacg
     5101 agcggcgcgc catgaccacc ggcggcgacg tcgcggacgc gcagatcgcc gatcgtgatg
     5161 atgcccgtgc gctcggcgat gaccgcggcc tgaccgattg ccagcgtcga gcgggtcgtg
     5221 accggctgat cgagccaggg cagcgagccg gtcgacggct ccgcggccgg acgcggacga
     5281 tgccagatcg tctgaccggc ggcggcgatg agatcgatgt cgtcattcgt caaccccgca
     5341 tcggcgatca ggcggttcgc cgcgctggcg aacagttcgc cgaggacgaa gtcgagatgg
     5401 cagatctctt cgatggtcca gagtgccgga tgctcgccgg tgtcgggatc ggtcagcttc
     5461 cgatcgcgtt cgcgttcgaa caggagcttg atgcgatgtt gaatctcctc ggggaactcg
     5521 aaactggcga atcgcagctt ctcgatacgc gctccgtcgc gcgcgttacc gtcgatgcgc
     5581 acgagcacgg cgtcgatggc gtcgacggat gtgcccgaca tcaagccgat cacgactcgc
     5641 gccttctttc cggcgatctt gatgaggcgc tcgacgcgat tcacgattcg agaactttcc
     5701 gcagattgcc gccgttcgcg gcgaggatcg ctttcgcctc ttcgagcgaa agccgtttac
     5761 cagccatcac cgacgccagc ttgacgttgc cgccagcgtc acgcattgcc tgcacggcag
     5821 ccggacggtt cacgcccgcc gcgagctcga ccatccgcac ggcccggctg cgcagctttt
     5881 cattcgttgc cggcatgtcg atcatcaggt tggagtgaac cctgccgagg agaatcatca
     5941 cgccggtgga gagcgtgttg aggacgagct tttgcgtggt accgctcttc attcgcgtcg
     6001 agcccatgat cacttccggc ccggtctgcg gcgcgatggc gatgtccgcc tcgctggcca
     6061 gaggagagct cggattcgat gtaattccga ccgtgagcgc acccagcatg ttcgcgcgcc
     6121 ggatggccgc tacggtgtac ggcgttgtcc ccgatgcggc gattccgacg acggcatctt
     6181 ccgctgtcac agccgcattc accgcttcga ttgccgcatc ttcatcgtcc tcggcacctt
     6241 caacagcgtg cagaaatgcg cgctcgcctc cggccatgat tacgctgacg agcgaggcgt
     6301 cgatgccata cgtcggcgga agttcggacg catcgagcat ggcgatgcgc ccgctcgttc
     6361 ctgcgccgac atagaagaga cgcccgccat gcgagagccg gtcggcgatc tgatcgaccg
     6421 catgtgcaat gcgatcaccc tcggccgcca cagcagcggc gaccagcgca tcctgttcct
     6481 ggatgatctg cacgatctct cgcgcggacc gccggtcgat atcgaggctg gcctcgagcc
     6541 gctcctggct catcgccgcg aagataccac tgcatgctga cggagcttcg ctatcacggt
     6601 cggctaacta cgaagtgcgt cgtatgatgt cggaatggag acggcggtcg ctggaacgcg
     6661 ggaacacagg cttgcaggcg acgccttcgt cggcacgccg cgcctcttcc gccaacatgc
     6721 gggcttctca ttcggccact ggctggctac tccggtcggc gacgatgttc gggagcatct
     6781 gcacgtcgcg gctcacttca tgttcatcgc gggcggccgc tttgtgactg ctgccgcagg
     6841 agcggaccgg gcgtcgccac tgatctacaa cccgcccaac accgttcatc gcgaccgctt
     6901 cgagtcatcg tcaggacgct tcttcgcggt ttcggcggac tctgcggcgt tctgcgacat
     6961 cgaaccgccg cccggcgttg ctcaccggat cgagaacgtc ggactgcacg cgatcgtcat
     7021 ccggctcatg cacgaatgcg aatggtggag cgatgattca ccggcgattg ccgaatctct
     7081 atgcgttgca ctgttcgcag ggatgaaccg tcgcgggcgc ttcgtcgccc ctcgctggct
     7141 cggcacggcc accgagcgac tgtgtgagga gtacgagacg ccagtcgcaa ttgcggacct
     7201 ggcgcgagac ctcggcgttc acaggaccca tctcacgcgc acgtttctac gtgcgcacgg
     7261 atgcacaccg gctgagtttg cgaggctgca tcgcatccga agggccgcca cgatgctcgc
     7321 gtcgacgcgt acaccgatcg ccgagatcgc tctggccagc ggcttcagcg atcag
//
LOCUS       DATLVW010000396         4149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_427179, whole genome shotgun sequence.
ACCESSION   DATLVW010000396 DATLVW010000000
VERSION     DATLVW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427179"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VNN25_11920"
     CDS             complement(<1..572)
                     /locus_tag="VNN25_11920"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HXH92278.1"
                     /translation="MILRFARVFASAVLLMLIAAPLFATFCVPPGTPAQPPPPDPTLP
                     VCEPAACHSCSTSPCYAKSGTYVNDFTDLQIPTAGMYSLTVSRRYDSSRPADGPLGVG
                     WSSSLTAHLYYATYLVSAPSTYSYEADVVMPDGVIYRFTTNGSAFTPPSGRYDTLVRN
                     ADETYSLTLQHTRSVYRFNTDGSLASLTDD"
     gene            complement(580..2658)
                     /locus_tag="VNN25_11925"
     CDS             complement(580..2658)
                     /locus_tag="VNN25_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXH92279.1"
                     /translation="MTRPAVAGKDEKRNVVGIRGVRAWAVVAVIVSATAAGYAQTFTI
                     TPSQVYVVECQETFVTLSGSNLTGASSTLVDFAGNSQVFELAPNTATPTQLEVWIPMA
                     VATTLGQYSVTVKTTDMNGATTTIGPAVLSVIARDGVAPVPSLPEVVVADATSSSGAF
                     VTFDVQGASCDHGSGALFPIGTTTVTCSVTNGFGTGTNSFSVVVNPLSGAPPILGIPE
                     IVVAEGTSPSGATVSFDPAGATCDHASGSLFPMGDTTVNCTQTNSFGTSTGKFLVVVT
                     DTIRPVLSLPSNISTGNSVVTYTATASDAIDGAITPLCSPASGSTFPNGSTVVQCMAT
                     DSHANSVSGSFTVTVTPPALSDFTASQNVYQLNPAAAGSVTYTSNVPLTLTETLTIRS
                     EATGATVRTIVNAVRTAGSYQDVWNGTNDGGQLVPDGAYRYFVTVSANGSTVTWDDGT
                     HFAGTTQTQLPYPKCRNASGELVDCGDASITFDPYTNMPLRINYCVGDGDPPACSGTT
                     PYLVTIKATNLGETDASCTSDCLLSAYQSSGAHEMAWYGSNRDGTFIGGAPGLTVIRR
                     NDIWPRNLTLVYGTAPAVSNFTISSPIFNPAAAPNVTSGETFGITVATFQGRSVTVTG
                     QFKNLSYGSVLRTLTTSSQQPGQVTLTWNGRADNGAWVAPGLYEATITVTDSAGASAI
                     LKPLITVRYE"
     gene            complement(2655..3887)
                     /locus_tag="VNN25_11930"
     CDS             complement(2655..3887)
                     /locus_tag="VNN25_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXH92280.1"
                     /translation="MRHSLNITLLLTSCLLVSPLIAGGPPVIGSISLKRETFNPKAGD
                     AVDLAVRRVPTATAAGRPIRSVNVDRQSLNVAAAEGATVRIDFGQAGRASVVIVDRDG
                     YPVRALLGAQPASGSVTLHWDGRDDSGRFVADEAYSLKIDWRGPEGTDTYFPANEPAA
                     MSAIEARSYDRRTATLVYSLPRASRVHIQAGTAAVDPKTKKPVGPVMKTLVNREPRVS
                     GTIAEHWNGYDESASIFVPDLKDFVVAIAATPLPENSIVTFGNRQRRFVDTLSLRQGP
                     SLFTDRRHQTHHTGLSTPDDVSPALVIEPLNAAWSATERAWIMNGTRALRLRLRVEGP
                     TAGAFRRHPATVELFVNGQRIGSPSRKTSDVVEIPLHPGGNLSRVSINWNSDWGPVAA
                     NTIQVRVDGPAPAAGGGK"
BASE COUNT          791 a         1222 c         1354 g          782 t
ORIGIN      
        1 taatcgtcgg tgagcgacgc gagcgaaccg tccgtgttga agcgatagac cgagcgcgtg
       61 tgctgcaacg tcaaggagta cgtttcgtcg gcatttcgga ctagcgtgtc gtaacgcccc
      121 gacggcggcg tgaacgcgct accgttggtt gtgaagcgat agatgactcc gtcaggcata
      181 acgacgtcgg cttcgtacga ataggtgctc ggcgccgaca ccagatacgt cgcatagtac
      241 aggtgtgccg taaggctcga cgaccatccg acgcccagcg ggccatccgc ggggcgactg
      301 ctgtcgtaac gccgagagac cgtgagcgag tacatgcccg cagttggtat ctgcaggtcg
      361 gtgaaatcgt tcacgtaagt gcccgactta gcgtagcagg gcgatgtcga gcaactgtga
      421 cacgccgccg gctcgcacac cggaagcgtc ggatcgggcg gagggggctg ggcgggagtt
      481 cccggcggta cgcaaaacgt tgcgaagagt ggagcggcaa tgagcatcaa taacactgcc
      541 gaggcgaata cgcgagcaaa tcgaaggatc atcggtttct cactcatagc gcaccgtgat
      601 gagaggtttc agaatcgccg aggcgccggc ggagtcagtg acagtgattg ttgcttcgta
      661 caaacccggc gctacccatg cgccattgtc ggcccggccg ttccaggtta gcgtcacctg
      721 gccgggctgc tgcgacgacg tcgtgagtgt ccgcaagacg gaaccgtacg agaggttctt
      781 gaactggccg gtcacggtca ccgagcgtcc ctggaatgtc gctacagtga tgccgaaggt
      841 ctcgccgctc gtcacgttcg gagctgccgc cggattgaat atcggcgacg agatcgtgaa
      901 gttggagacg gcgggcgctg tgccgtaaac gagcgtcaga ttccgcggcc agatatcgtt
      961 gcgccggatg accgtcagcc cgggcgcgcc gccgatgaac gtcccgtcgc ggttcgatcc
     1021 ataccacgcc atttcgtgcg cgccactgga ctggtaggcg ctcaacaggc aatccgatgt
     1081 acagctcgca tccgtctcac caagattggt ggccttgatg gtaacgaggt agggcgtggt
     1141 tccgctgcac gccggaggat ctccatctcc gacgcagtag ttgatccgaa gcggcatgtt
     1201 cgtgtaaggg tcgaaggtga tactggcatc accgcagtcc accagctcgc cggaggcatt
     1261 gcggcacttc ggatacggga gctgcgtttg ggtggttccg gcaaagtgcg ttccatcgtc
     1321 ccaggtcaca gtagagccat tcgccgatac cgtgacaaag tagcggtatg cgccgtcagg
     1381 tacgagctgg ccgccgtcat tcgtaccgtt ccagacgtcc tgatagctcc cggccgtccg
     1441 cacggcgttc acgattgttc tcaccgtggc accggtcgcc tccgatcgga tcgtgagcgt
     1501 ctccgtgagc gtgagcggga cattcgaggt gtacgtgacc gaccccgctg cggccgggtt
     1561 cagttgatag acgttctgcg atgccgtgaa atccgagagc gccggaggag tcaccgtgac
     1621 ggtgaagctt cccgagaccg agttcgcgtg cgagtcggtc gccatgcatt gcacgaccgt
     1681 cgagccattt gggaacgtcg agccggaggc aggactgcac agcggtgtaa tcgcgccgtc
     1741 gatggcgtcc gaggccgtgg ctgtgtaggt gactacgctg tttcccgtgc tgatgttcga
     1801 gggaagcgag agaaccgggc ggatggtgtc ggtcacgacg acgaggaact tgcctgtcga
     1861 agtaccgaag ctgttggttt gcgtgcagtt cacggtggta tcgcccattg gaaagagcga
     1921 accggaagcg tgatcgcagg ttgcgccggc aggatcgaac gaaacagtgg ctcctgaggg
     1981 cgaagttccc tccgcgacca cgatctcggg gatccccaga atcggcggag ctccggacag
     2041 cgggttcacg accacggaaa aggaattggt tccggtgccg aagccgttcg tgaccgagca
     2101 ggtaacggtc gtcgtgccga tcgggaacag cgcgccggat ccgtggtcgc agctcgcgcc
     2161 ctgtacatcg aaggtgacga acgccccgga cgacgacgtc gcatcggcga cgaccacctc
     2221 agggagagaa ggtaccggcg caacgccgtc gcgggcgatc acgctcagaa cggcaggccc
     2281 gatggtggtc gttgcaccat tcatatcggt cgtcttcacc gtgacggagt actggccaag
     2341 tgtcgtggca acggccatcg gaatccacac ctcgagctgg gtaggcgtgg cggtgttcgg
     2401 cgcgagctcg aaaacctgac tgttgcccgc gaagtcgacg agcgtcgatg atgcgccggt
     2461 tagattgctg ccgctcagtg tgacgaatgt ctcctgacac tcaaccacgt acacctgcga
     2521 gggcgtgatc gtgaacgtct gggcatagcc cgccgcggtc gccgacacga tgacagcaac
     2581 aaccgcccaa gcgcgcactc cccgtatgcc aacgacgttg cgtttctcgt ccttgccggc
     2641 gacagccggt cgtgtcattt cccacctcct gcggcgggcg caggtccgtc aacgcggacc
     2701 tgaatggtgt tcgccgcaac cggcccccaa tcgctgttcc agttgatgga gacacgcgag
     2761 aggtttccgc ctggatggag tgggatttcg acaacatcgc tcgtcttccg cgatggactg
     2821 ccgatgcgct gcccgttgac aaagagctca accgtcgccg ggtgtcgccg aaaagcgccg
     2881 gcggtcggtc cttctaccct gagtcgcagc cggagcgccc gggttccgtt cataatccac
     2941 gcgcgctcgg tcgccgacca cgccgcattc agcggctcga tcacaagtgc tggcgagaca
     3001 tcgtcgggag tgctgagccc agtgtggtgc gtttggtgcc gacggtccgt gaacagcgaa
     3061 ggtccttggc gcaggctcag cgtgtcgaca aaccgacgct gccggttacc gaatgtgacg
     3121 attgagttct ccggcagcgg cgtcgcggcg atggcaacga cgaaatcctt gaggtccggc
     3181 acgaagatgc tggcactctc gtcatatcca ttccaatgct cggcgatcgt tccactcact
     3241 cgcggttcgc gattgacgag ggtcttcatg accggcccga ccggcttctt cgtcttcgga
     3301 tccacggccg cggttccggc ctggatgtga acgcgcgacg cgcgaggaag gctatagaca
     3361 agcgttgccg tccgccggtc gtacgaacgt gcttcgatgg cgctcatcgc cgcaggctca
     3421 ttcgccggaa agtacgtgtc tgttccctca ggaccgcgcc agtcgatctt gagcgaatac
     3481 gcctcatcag cgacaaatcg gccgctgtcg tcgcggccat cccaatgaag cgtgactgag
     3541 ccgcttgcgg gctgagcacc gaggagagcg cggacagggt agccgtcgcg gtcgacgata
     3601 acgaccgagg cccgtcctgc ctgaccaaag tcgatcctga cggtggcgcc ctccgctgca
     3661 gcaacattca gagactgccg atcgacattg accgaccgaa ttgggcgccc agcagcggtg
     3721 gcggtgggga ctcgccggac ggcgagatcg accgcgtccc cggctttcgg gttgaacgtt
     3781 tcgcgtttca gcgaaatgct accgatcacg ggtgggccac cagcgatgag cggcgagacc
     3841 aaaaggcagg acgtcagtag gagcgtgatg ttcagcgaat gccgcatcgc caatctccat
     3901 ataccgctat tgaaaggccc ggacagcggt tgcgatcgag aagactgtca ataaagccgc
     3961 gttaatgtcc tgataacctt ggcacgatac actgcggcac tgtcaagtgc gagtcccgtt
     4021 ttttgacttt tactttcgcc ggtactcttc cggggtcatt ccgaattggt cggcgaagag
     4081 gcggtagaag gtcgtgcggg tgccgaggcc ggtgattacg gcgatgtcgt cgacggtgct
     4141 ggtcgtgcg
//
LOCUS       DATLVW010000397         5459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_595061, whole genome shotgun sequence.
ACCESSION   DATLVW010000397 DATLVW010000000
VERSION     DATLVW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595061"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VNN25_11935"
     CDS             complement(<1..733)
                     /locus_tag="VNN25_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92281.1"
                     /translation="MRHATRVARTTAARAAAVFLLLAGVAWLTTHLIVSGIEAEFSAR
                     SSRHLEREVQRVRSEIAATESKLDAAVGRVAKKLKVQPAATRDAMFAMLHGEATGVRQ
                     GIRIVAPNGEALAWWGEDLRTPGVTAFEFDATNLYIVRSQPLPNPAVSVQAFERVPNQ
                     PKSRSLFDLDDDWVVGTMFHAGVLRQEPGSLRFVLERRPSATLWIDVVPRAKPDVIQS
                     VRALGNGIAAILLALSALAVLWGVGS"
     gene            complement(817..1248)
                     /locus_tag="VNN25_11940"
     CDS             complement(817..1248)
                     /locus_tag="VNN25_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92282.1"
                     /translation="MRLSTKMALLLLFVPLVASAQYRDLDVALSNLTRGFGSGDPQAI
                     VAGMAAGDQVMLQFPGLIEQNGFFGRDQAAYLLDGLFGKVHPSAFEQQNAKKVSAEAQ
                     YHITGTWTIQNAGKPETRELYITLHSKDGLWSVVSIRSAGK"
     gene            complement(1312..1980)
                     /locus_tag="VNN25_11945"
     CDS             complement(1312..1980)
                     /locus_tag="VNN25_11945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXH92283.1"
                     /translation="MLDILYEDDALLFVNKPAGIVVQRGYDAEEPVLLDIAAAYAGTI
                     FLMQRLDRGTSGVMFFSKLASINANLTRQFEGKRIRKRYLALCEGELGERQTIDAPLI
                     RIGPISFGAGDGGKRAITIVTPVRSTRTGSFVAVDLLTGRTHQIRVHLAAIGHPLIGD
                     WLYGQRNDVRPMLHARELSMTHPLTNEPLRVEAPLPDDFRNTASERGIVSRGEDIMTL
                     DGCS"
     gene            complement(1984..3156)
                     /locus_tag="VNN25_11950"
     CDS             complement(1984..3156)
                     /locus_tag="VNN25_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXH92284.1"
                     /translation="MTVQPGLEVLLDDPRPIAGKKIGLVTNQSGVTGDLRHVVDLLHR
                     GTGWKLTTLFGPEHGIWGEAQDMAHVGHSVDPMTGLTVYSLYGASENDLAPRQELLHG
                     LDALVIDLQDIGSRYYTFIYTMALCMREAGKAGVQVVVLDRPNPIDGVHLEGNIREDK
                     YSSFVGMFALPTRHGMTAGELARYFNKVFKLDCDLVVVPMKGWQRSMWWDETGLPWVI
                     PSPNMPTVGTATVYPGMCLIEGTNLSEGRGTTHPFELFGAPWLEPFQLAEKLNAIGLP
                     GVRFRPHYFMPTFQKHAGKVCGGVELHVTNRAIFEPFRTGLWCVKVARDIDPQQFDWR
                     RETYEFVSDRLAIDLLAGSARYRELVESGGNIEDWGAEWKEPLREFAKTREEFLLY"
     gene            complement(3153..3632)
                     /locus_tag="VNN25_11955"
     CDS             complement(3153..3632)
                     /locus_tag="VNN25_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HXH92285.1"
                     /translation="MIKARVITCSDAASRGERVDRSGPAVRELLTANGYDVDSIVIVS
                     DDIETIAGAIEAATGDGIRLVVTTGGTGIAARDVTPEATMWVCEKVIPGFGELMRAAS
                     LTKTAMAPLSRAQAATRGSALIVNLPGSESGARENLQAILHLIPHALQLLEGKTEHQ"
     gene            3716..4879
                     /gene="queA"
                     /locus_tag="VNN25_11960"
     CDS             3716..4879
                     /gene="queA"
                     /locus_tag="VNN25_11960"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXH92286.1"
                     /translation="MLRTDFAYDLPSSLIAQEPRERGRSRMMVVSPQAGAEPRIEHDA
                     FTSFPDRLVPGDVLVINDTRVIPARLFAKPKPGMSRPIEILLTRRLTEAQTRVSVPHG
                     SPPLAPSVLSSPHEVNDVAQTLLSVQEIWEAWCKPAKRVKVGDELVFSDTLRARVLAK
                     NEGTVTIAFESDGDIDAEIERIGIPPLPPYIARETPRDEDRSAYQTVYAAERGAIAAP
                     TAGLHFTPEILERIAARGVEIVRITLHVGIGTFKPVKVDDIAEHVMDEERYEISSDAA
                     SRLNAALNEHRTIVAVGTTSVRTLESAIRAGDGHFVAGRAATSIFITPGFGFRAVDKL
                     LTNFHLPESTLLMLVSAFAGIETIRRAYAEAIGNQYFFYSYGDCMFLNGRGSW"
     gene            4888..>5459
                     /locus_tag="VNN25_11965"
     CDS             4888..>5459
                     /locus_tag="VNN25_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXH92287.1"
                     /translation="MKRILAVLLTVVFVAIPSFAAQTPPPAPVTDKKAEPKKPEKGEQ
                     EPRPVAQEQQKQDSQKKDAPAPSAATQPSPNPIAAEGAAEKKEDKKWDVNNPPGPQYD
                     VPIDVTEGTWLSLDVSPDGNEIAFDLLGDIYTIPMSGGEAKSITTGIAWDMQPRYSPN
                     GKWIAYTSDRAGGDNIWIMNRDGSKPQQVT"
BASE COUNT         1066 a         1682 c         1701 g         1010 t
ORIGIN      
        1 cactccccac cccccacaac actgccaacg cgctaagtgc gagcagaatc gcggcgatgc
       61 cgttgccgag ggcgcggacg ctttggatga cgtcgggttt cgcgcgtgga acgacgtcga
      121 tccagagcgt cgccgacgga cgacgctcca gaacgaagcg cagcgatcct ggttcctgcc
      181 gcagaacgcc cgcgtgaaac atcgttccga cgacccaatc gtcgtcaaga tcgaagagcg
      241 agcgcgattt gggttgattg gggacgcgct cgaacgcctg gacggagaca gcgggattcg
      301 ggagaggttg cgaacggacg atgtagaggt tcgtggcgtc gaactcgaag gcggtcacgc
      361 ccggcgtgcg aaggtcttcg ccccaccatg ccagcgcttc tccgttcgga gcgacgatgc
      421 ggattccctg gcggacgccg gtggcttcgc cgtggagcat cgcaaacatc gcatcacggg
      481 ttgccgccgg ctgcaccttc agtttcttgg caacgcggcc gacggcggca tcgagcttcg
      541 actcggtagc cgcgatctcg ctccgtacgc gctgcacttc gcgctcgagg tgccgcgaag
      601 aacgagcgct gaactccgct tcgatccccg agacgatcag gtgagtcgtg agccaggcga
      661 ccccagcgag caaaagaaag accgccgcgg cgcgcgcagc ggtcgtcctg gctacccggg
      721 ttgcgtgccg catggtgatg aagaatcaat gaaccacaga gacgcaaaga tcacagaggt
      781 ttctctgtgt ctctgtggtt atgcctttga gagcaatcac ttccccgccg agcggatcga
      841 aaccaccgac cagagaccat ccttgctatg cagagtaatg tacagctcgc gcgtctccgg
      901 ttttcccgcg ttctggatcg tccacgtgcc ggtgatgtgg tactgcgctt cggcgctcac
      961 tttcttcgcg ttctgctgct cgaatgccga ggggtggact ttgccgaata gaccatcgag
     1021 gagatacgcc gcctgatcgc ggccgaagaa accgttctgc tcgatgaggc cggggaactg
     1081 gagcatcacc tgatctcccg cggccatgcc ggccacgatt gcctgcggat cgccgctgcc
     1141 gaatccgcgc gtcaggttcg agagcgccac gtcgagatcc cggtactggg ccgacgcgac
     1201 gagcggcacg aacagaagca gcaacgccat ctttgtcgag agtctcatcg tgttctccaa
     1261 tgcctgctgt aacggcctgt ggtaaggcat tattctaaca gattcaataa gttagctgca
     1321 cccgtcaagg gtcattatgt cctcgccgcg ggacacgatg ccgcgctccg aggcggtgtt
     1381 gcgaaagtca tccggaaggg gtgcttcgac ccgaagtggc tcattcgtca gtggatgcgt
     1441 catcgaaagc tccctggcat ggagcatcgg ccggacatcg ttgcgctgcc cgtacagcca
     1501 gtcgccgatg agcggatgcc cgatcgcggc gagatggacc cggatctggt gcgtgcggcc
     1561 ggtgaggaga tcgacggcta cgaatgagcc ggttctcgtg gagcgaaccg gcgtgacgat
     1621 ggtgatggcg cgcttgccgc cgtcgccggc accgaatgag atcggcccga tacgaatcag
     1681 cggcgcatcg attgtctgcc gttcgccgag ctcaccttcg caaagcgcga ggtagcgttt
     1741 gcggatgcgc ttcccttcga actgccgggt gagattggcg ttgatggacg cgagcttgga
     1801 gaagaacatc acgccgctcg ttccgcggtc gagccgctgc atgagaaaga tggtcccggc
     1861 gtacgccgcc gcgatgtcga gaaggaccgg ctcctccgcg tcatacccgc gctgtacgac
     1921 gatgccggca ggtttgttga cgaagaggag agcgtcatct tcgtaaagaa tgtcgagcat
     1981 gcatcagtac agcaggaact cctcgcgcgt cttcgcgaac tcgcgcagcg gctccttcca
     2041 ctcggcgccc caatcctcga tgtttcctcc gctctcgacg agctcgcggt agcgcgcgct
     2101 gccggcgagg aggtcgatgg cgagacgatc ggagacgaac tcgtacgtct cgcggcgcca
     2161 gtcgaactgc tggggatcga tgtcgcgggc gactttcacg caccagaggc cggtgcggaa
     2221 gggctcgaag atggcgcggt tcgtcacatg aagctcgacg ccgccgcaga ccttgccggc
     2281 gtgcttctgg aacgtcggca tgaagtagtg cggacggaag cggactccgg gcagaccgat
     2341 tgcgttgagc ttctccgcca gttggaacgg ctcgagccac ggtgcgccga agagctcgaa
     2401 tggatgcgtc gtgccccgtc cttcgctcag gttggtccct tcgatcagac acatgccggg
     2461 atagacggta gcggttccga cggtcggcat gttcggggag ggaatgaccc acggcaatcc
     2521 ggtctcgtcc caccacatcg agcgctgcca tcccttcatc ggaacgacga cgagatcgca
     2581 atcgagcttg aaaaccttgt tgaagtaacg cgccagctca cctgcggtca tgccatgccg
     2641 cgtcggcagc gcgaacatgc cgacaaagct ggagtacttg tcttcgcgga tgttgccttc
     2701 gagatgaacg ccgtcgatcg gattgggacg atcgagcacg acgacctgta cgccggcttt
     2761 ccccgcttcg cgcatacaga gcgccatcgt gtagatgaag gtgtagtagc gcgagccgat
     2821 gtcctgcaga tcgatgacga gcgcgtccag tccgtgcaac agttcctgcc gcggcgcgag
     2881 atcgttctcg ctcgccccgt agagcgaata aacggtgaga ccggtcatcg gatcgacgga
     2941 gtgcccgacg tgcgccatgt cctgcgcctc gccccagatg ccgtgctcgg gaccgaagag
     3001 cgtggtgagc ttccacccag tgccgcgatg gagcagatcg acgacatggc gcagatcgcc
     3061 cgtgacgccg ctctggttgg tgacgaggcc gatcttcttg ccggcgatcg gccgcgggtc
     3121 atcgagaagt acttccagtc cgggttggac agtcattgat gttccgtttt cccttcgagt
     3181 agttgcagcg cgtgcggaat gagatgcagg attgcctgca ggttctccct cgcgccgctc
     3241 tcgcttcccg gtaggttcac gatgagtgcc gacccgcgcg tggccgcctg cgcgcgcgac
     3301 aacggggcca tcgctgtctt cgtcagcgaa gccgcacgca tgagctcccc gaaccccgga
     3361 atcactttct cgcacaccca catggtcgcc tcaggagtga cgtcacgcgc cgcgattccg
     3421 gtaccgccgg tggtgacgac gagacggatc ccgtcaccgg tcgcggcttc gatcgcacca
     3481 gcaatggttt cgatgtcgtc ggacacgatg acgatcgaat cgacgtcgta tccgtttgcg
     3541 gtcagcaatt cacgaacggc cggtccggag cggtcgacac gctcaccgcg cgacgcagca
     3601 tcggaacagg tgatgacgcg ggctttgatc atcgacgcgg attctatgcg tgcattgggt
     3661 gtcacgcgaa ggcgcgaaga cgcgaagggg gaggaacggt gaataatcaa cttccatgct
     3721 ccgcaccgac ttcgcctacg accttccttc atccctgatc gcgcaggagc cgcgcgaacg
     3781 tggtcgttca cggatgatgg tggtgtcccc gcaggctggt gccgagccgc gcattgagca
     3841 cgacgcgttc acgagttttc ctgatcgtct cgtacccggc gatgtcctcg tgatcaatga
     3901 cacccgtgtg attcctgccc ggctctttgc gaagccgaaa ccgggaatgt cgcggcccat
     3961 cgaaattctg ctgactcgca ggctgactga agcacagaca agagtgtctg tgccacatgg
     4021 ctctccgcct cttgcgccct cggtcctgtc ttcgccacat gaagtgaatg atgtggcaca
     4081 gacactcttg tctgtgcaag aaatctggga agcctggtgc aaacctgcca agcgcgtgaa
     4141 ggttggtgat gaactggtct tcagtgacac cctgcgagcg cgtgtcctcg cgaaaaacga
     4201 gggcaccgtc acgatcgcgt tcgagagtga cggtgatatc gatgctgaga tcgagcgcat
     4261 cggcatccca ccacttccgc cctacatcgc acgcgaaacg cctcgcgatg aagatcgcag
     4321 cgcttaccag actgtctacg ccgccgagcg cggcgctatt gctgcgccga ctgccgggct
     4381 tcacttcacg cccgaaatcc tcgaacgcat cgccgcccgc ggagtcgaga tcgtccgaat
     4441 cacgctgcac gtcggcattg gtacgttcaa gccggtaaag gtcgacgaca tcgcagagca
     4501 cgtgatggat gaggagcgct acgagattag cagcgatgcc gcttcgcggc tgaatgcagc
     4561 gctcaatgaa catcgcacca tcgtcgccgt cggcaccacc tcggtgcgca cactcgaaag
     4621 cgcgattcgc gccggcgatg ggcactttgt tgccggccgc gcggccacat cgatcttcat
     4681 cacccccggt ttcggcttcc gcgccgttga caaattgttg accaactttc atctgccgga
     4741 gtcgacgctg ctgatgctag tctccgcctt cgccggcatc gagacgatcc gccgcgcgta
     4801 tgccgaggct atcggaaatc agtacttttt ctacagctac ggcgactgca tgtttctgaa
     4861 cggccgcggg agttggtaag gagaatcatg aaacgaattc tggccgtgct gctgaccgtc
     4921 gttttcgtcg caatcccttc cttcgcagcg caaacgccgc cgcctgctcc ggtaaccgac
     4981 aagaaagccg agccgaaaaa gccggaaaag ggcgagcagg agccacgccc ggtcgcgcag
     5041 gagcagcaga aacaggattc gcagaagaaa gacgcgcctg cgccatccgc tgcaacgcag
     5101 ccatcgccga atccgatcgc ggccgagggc gctgccgaga agaaagaaga caaaaagtgg
     5161 gacgtcaaca acccgccggg gccgcagtac gacgttccga tcgatgtgac cgaggggacg
     5221 tggctgtcgc tcgatgtgag tccggacggc aacgagatcg ccttcgatct tctcggcgac
     5281 atctacacca ttccgatgtc cggcggagaa gcgaagtcga tcacgactgg tatcgcctgg
     5341 gacatgcagc cgcgttactc gccgaacgga aagtggatcg cgtacacgag cgaccgcgcc
     5401 ggcggcgaca acatctggat catgaaccgc gacggctcga agccgcagca ggtgacgaa
//
LOCUS       DATLVW010000398         5032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_143729, whole genome shotgun sequence.
ACCESSION   DATLVW010000398 DATLVW010000000
VERSION     DATLVW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143729"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..818
                     /gene="hypB"
                     /locus_tag="VNN25_11970"
     CDS             141..818
                     /gene="hypB"
                     /locus_tag="VNN25_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HXH92288.1"
                     /translation="MSSEPRLVEVRRNVMKKNDVLARALRERYRRDGVFVVSLVSSPG
                     SGKTTLLEKTLTELGKTHRVAALVGDLATDNDAARLARSGAPVKQITTGTVCHLDAEM
                     VNDALDHWPPSAPDFLFIENVGNLVCPSSYDLGENVRAVALSVTEGEDKPRKYPTIFN
                     SADIAIITKSDLAEAAEFDWEEAERSIQSVRPGMTITRLSAKKGEGMPEWIGMLVDRR
                     ERWLREA"
     gene            988..2712
                     /locus_tag="VNN25_11975"
     CDS             988..2712
                     /locus_tag="VNN25_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92289.1"
                     /translation="MWQRMTGIPTQLSGASILLSDGTVLAQDFDDQYRWWRLTPDAHG
                     DYFNGRWSHGPRMVHGHVDGATSVAPLLSGAFIAVSSQPGDPASDSFSPIAGPAEVVS
                     AGGLEWSAEPVAREWTAVTEVLCTLPDGSVLAAGFDFGVSVDGHVWRWTPAEGWSVYV
                     DLPADVPVFASLTLLPDGTVLALGRTNEYRLVGREWSIGSAHGLRLDDAWDCVTGPVC
                     VLGKGGVLAFDYLGGMAFRSIGGIWSPAGQLPDRFGRSPAAYAAPACLLPSGRVLVGA
                     EYPFSDREGIDIQVAYFEWDPVTGLHDAGSIEPDGTARNVEPPELRRMLMLPNGWVLV
                     TRIGDIALYQPDPPSTQPEWAPRITAIDEIRIPSSERIPLTIGQHRRLHGVRLTGPSQ
                     GCVGPFNKGNPTNYPLVRIEYPDGRVFYLRTSDHSTLGIGPGEEPQSTTFLVPDWLPD
                     GDADLCVVVNGLKDCIPVRMLQPHPRRFPGYQQLVGSLADGALWILTNGGFQPTPPGP
                     NDGRIRAAYEKILAGIEELSRANDADPLRIHELQGIPGMSSILGGIRALRSAGRGVTK
                     RGSTKREQ"
     gene            2899..3396
                     /locus_tag="VNN25_11980"
     CDS             2899..3396
                     /locus_tag="VNN25_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3034..3036,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXH92290.1"
                     /translation="MPPQTPPKIGQQAPTFTLRDQNGKRVSLADAAGTKVVLVFYRGY
                     WUAYCVRQVVGLRDLAHAYPDVKFYAISPDSPEESRKFAALVASDHRGALTFSLLSDP
                     LSRVIDRYNLRDEAYAGKKEDGIPHPAVFVLDTKGRIRWTKIETDFRERPTNAEIAAA
                     IDAME"
     gene            4529..>5032
                     /locus_tag="VNN25_11985"
     CDS             4529..>5032
                     /locus_tag="VNN25_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92291.1"
                     /translation="MNPNQETMNPSAAADLPQAAATLSPEGVVDQLRATRAQIGEMTP
                     LTVAQRKALHGRIRMSNPILQASINVIGALDIVAKAIGQPADDMRLLCDETNRWTAVE
                     DELRTMLNGVSGANLIRRHRLSLIAGQAYNIGSKLARDPAHAVLVPHVQEIKRLRSFL
                     RRKKVSPA"
BASE COUNT          853 a         1684 c         1605 g          890 t
ORIGIN      
        1 gtgctcgttc agtgccccgt ctgtcatgag ccgcgttctc ctcattcctt gtggcggctc
       61 gaatgcgcga cttgcgatac gcctgccgcc ggcattgcac aaggaaggga ccttcagatc
      121 ttcgcaatgg aagtcgtctg atgagctccg agccgcgcct ggttgaagta cggcggaacg
      181 tcatgaagaa aaatgacgtc ctggcgcgcg cgttgcgcga gcgctaccgc cgcgacggcg
      241 ttttcgtcgt cagcctcgtc tcgagccccg gctccggaaa aacaacactg ctcgaaaaga
      301 cgctgacaga gctcgggaag acgcaccgcg tcgccgccct cgtcggcgat ctggccaccg
      361 acaacgacgc cgcccgcctc gccagaagcg gcgctccggt gaaacagatc acgaccggca
      421 cggtctgcca cctcgacgcc gaaatggtga atgacgcgct cgatcactgg ccgccctcgg
      481 ctcctgattt cctcttcatc gaaaacgtcg gcaatctcgt ctgcccctcc agctacgacc
      541 tcggcgaaaa cgtccgcgcc gtcgcccttt cggtcacaga aggggaggac aagccgcgca
      601 agtacccgac gatcttcaat tccgccgaca tcgccatcat caccaagagc gacctcgccg
      661 aagcggccga attcgactgg gaggaggccg agcgcagcat ccagtccgtc cgccccggaa
      721 tgacaatcac gcgactctcc gcaaaaaagg gggaaggcat gcccgaatgg atcggaatgc
      781 tggtcgatcg ccgcgaacga tggctacgcg aagcatagga gtgcgggcgt ctcgcccgcg
      841 gcggcgaggc gtctcgcctc ggcgtggttt ccgcgacgcc ggataccttt tcccgcagcg
      901 cacgtttcac cgccgggcga gaagcccatc ggcagccggc gagacgcctg cactctcggc
      961 gaaacgaaca aacagggagg tgtgaccatg tggcagagga tgaccggcat cccgacgcaa
     1021 ctgagcggtg cctcgatcct gctcagcgat ggaactgttc ttgcgcagga ctttgatgat
     1081 cagtaccgct ggtggcggct gacaccggac gcgcacggcg attatttcaa cggccgctgg
     1141 tcccacggac ctcgcatggt gcacggccac gtcgatggcg ctaccagcgt cgccccgctc
     1201 ctgtccggcg ccttcatcgc agtttccagc cagcccggtg accccgcgag cgattcgttt
     1261 tcgccgattg cgggccctgc cgaggtggtc tctgccggcg ggctggaatg gtccgcggag
     1321 ccggtcgccc gtgaatggac cgcggtaacc gaggtgcttt gcacgcttcc ggatggctcg
     1381 gtgctcgccg ccggcttcga cttcggagtg tcggtcgacg gccatgtctg gcggtggact
     1441 cctgccgaag gatggtcggt ctacgtcgat cttccggcag acgtgccggt cttcgcttca
     1501 ctcacactcc ttcccgacgg caccgtcctg gccctcgggc gcacgaacga gtaccgcctc
     1561 gttggccgcg agtggagcat cgggtccgcg catgggctgc gtctggacga tgcctgggat
     1621 tgtgtcaccg ggccagtctg cgtcctcggg aaggggggcg ttctcgcatt cgactatctg
     1681 ggcggcatgg cctttcgctc gatcggtggc atatggtcgc ctgctggtca gttgcccgat
     1741 cgttttgggc ggtcgcccgc tgcatacgcg gccccggcgt gccttctgcc atccggccgc
     1801 gtgctcgtcg gtgccgaata cccgttcagc gatcgcgagg gcatcgatat tcaggttgca
     1861 tatttcgagt gggatcccgt caccgggctt cacgacgccg gcagcatcga gccggatgga
     1921 acggcccgga atgtcgagcc gccggagttg cgccgcatgc tcatgctccc gaacgggtgg
     1981 gtgctggtca ctcgcatcgg ggatatcgcg ttgtatcaac ccgatcctcc ttcaacgcag
     2041 cccgaatggg cgccgcgaat caccgccatc gacgagatac gtattccgtc gtctgagagg
     2101 attcctctca ccatcggcca gcaccgccgt ttgcacggag tgcgtctcac cggtccgtcg
     2161 caaggctgcg ttggtccatt caacaagggc aatcccacga actatccgct cgtccggatc
     2221 gagtatcccg atgggagggt cttctacctg cgcacgagcg accacagcac gctgggcatc
     2281 ggcccgggcg aggagccgca atccacgacc tttctcgtgc ccgactggct acccgatggc
     2341 gatgcggatc tctgcgtggt ggtgaacgga ctgaaggact gcattcccgt tcgcatgctc
     2401 cagccgcacc cccgacgctt ccccggctac cagcaactcg tcggcagcct cgccgacggg
     2461 gcgctctgga tcctgacgaa cggcggcttc caacccacgc ccccgggacc aaacgacggc
     2521 cgcatccgcg ccgcgtacga gaagatcctc gccgggatcg aggagttgtc gcgcgccaac
     2581 gatgcggacc cgctgcgcat ccatgagctc cagggcatcc ccggcatgag cagcatcctc
     2641 ggcggaatcc gagcgctgcg aagcgcaggc aggggagtaa cgaaacgcgg cagtacgaag
     2701 cgcgagcagt gagtcaagtt tcgcgtggga acgccggcgt ttcgccgccg cgatctgcga
     2761 aagccccact tcttcaacgg gctcgcatgg caccaccacg tccacagcct gatatcgttg
     2821 catcgcttgc ccttcctcgc cgcgctcctc ctagtcctcg ctgcgaacac gtccgccgca
     2881 ccaacgccgc gtgccgtcgt gccaccgcaa acgccgccga agatcggtca gcaggcgcca
     2941 accttcacgc tccgcgatca gaacggcaaa cgcgtcagcc tcgcagacgc cgccgggacg
     3001 aaagtcgtcc tcgtcttcta ccgcggctat tggtgagcgt attgcgtccg gcaggtagtc
     3061 ggacttcgtg acctcgccca cgcgtacccc gacgtgaagt tctacgccat cagccccgat
     3121 tcgcccgaag agagccgcaa gtttgccgcg ctcgtcgcct ccgatcaccg cggcgcgctg
     3181 acgttctcgc tcctctccga cccgctctcg cgagtcatcg accgctacaa cctgcgcgac
     3241 gaagcgtacg ccggcaagaa ggaggacggc atcccgcacc ccgccgtctt cgtgctcgac
     3301 acaaaaggcc gcatccgctg gacgaagatc gagaccgact tccgcgagag acctacgaac
     3361 gccgagatcg cggcagcgat cgatgcgatg gaataggggg atcaccgctc atcggaacct
     3421 ggaataagga cgcccgttgt tttagggtcg tggccttccg taaattgacc ggcagcacgg
     3481 tcggaagcaa acgctacctc ggctaacgtt gctcgcgaac cgcgcggcgt ccagccggac
     3541 gcgtggggcg cccggtcaga cgcatgcggc gtcgatccgc atgtgtgtgg cgtcgggtca
     3601 gacgtgtgcg gcgtcgatcc gcacgtgtga ggcgtccggt cagacgcgtg cggcgtccat
     3661 ccgcgcgtgt tggtcgtcca gtcagacgcg tgcggcgtcc atccgcgcgt gtggggcgtc
     3721 cggtcagacg cgtgcggcgt cgatccgcac gtgtgaggcg tccggtcaga cgcgtgcggc
     3781 gtccatccgc gcgtgttggg cgcccggtca gacgggtgcg gcgtcgatcc gcacgtgtga
     3841 ggcgtccggt cagacgcgtg cggcgtccat ccgcgcgtgt ggggcgtcca gtcagacgtg
     3901 tgcggcgtcc atccgcgcgt gttgggcgtc cagtcagacg cgtgcggcgt cgatccgcac
     3961 gtgtgcggcg cccgggcgga cctgtggatc gtccagctca gacggtgggt cgctcagccg
     4021 gcgcggacgg tcatcgggcg cgagccgtca gccatcacga cggagcagtt tgtcgccggg
     4081 tctacgcgta aagtcgtcgg gtttgcgcgg acagcaattg gtgtttgccg atgttgtgtc
     4141 ctcggaatgc tggcggcgac tcagtaacgc agcatgcacg gcgacgccgc ccacttgaca
     4201 tcgcccacca ggctctgctg caatacgcgg cctcgtgggg ctggcatgcc ggcttctcga
     4261 gactggagtt ggcgctcgtg agagtcccac gctcctggcg gccaacgggt gagaacgacg
     4321 gcgctggaaa gtgcgactgg tctcatccct gcgggagttg agtggtgctc ctcctgccgc
     4381 gggctctcga agtgcgcgtg cacttcgggg aacatcaacc ggaagagcgg cgcgtcgccg
     4441 tcttttccag ccggtggccg cctgcacttg tgcacaggtg ctcgtcgttc ccctccggcc
     4501 taacaactat tgttctgagg agaacgatat gaatcccaat caggaaacca tgaatccgtc
     4561 tgccgccgcg gaccttccac aggcggctgc cacattgtcc cccgaggggg tcgtcgacca
     4621 gctgcgcgcc acgcgcgcgc agatcggcga aatgacaccg ttgacggtcg cgcagcgaaa
     4681 agcgctgcac gggcgcatcc gtatgtccaa tccgatcctg caggcgtcga tcaatgtcat
     4741 cggtgcgctc gacatcgtcg cgaaggcgat cggccagccg gcagacgaca tgcgtctgtt
     4801 gtgcgacgag accaaccgct ggacggcagt ggaggatgag ctgcggacga tgctgaacgg
     4861 cgtctccggg gcgaatctca ttcgccgcca ccgcctctct ctcatcgccg gacaggcata
     4921 caacatcggc tcgaagttgg cgcgcgatcc ggcccacgcc gttctcgtgc cgcatgtgca
     4981 ggagatcaag cgcttgagga gcttcctgcg ccgcaagaag gtgtcgccgg ct
//
LOCUS       DATLVW010000399         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_225787, whole genome shotgun sequence.
ACCESSION   DATLVW010000399 DATLVW010000000
VERSION     DATLVW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225787"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VNN25_11990"
     CDS             complement(<1..238)
                     /locus_tag="VNN25_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH92292.1"
                     /translation="MSALPLDGRVAVVTGGTSGIGLALVRGLADAGADVAPISRRHEQ
                     VDTAANEVAARGRRTARIVADVSDRASLDAALELA"
     gene            complement(235..990)
                     /locus_tag="VNN25_11995"
     CDS             complement(235..990)
                     /locus_tag="VNN25_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXH92293.1"
                     /translation="MKKEKDEPSAHAIDIQSADQVVRHVAVLIEGGKLRPGDRLVPER
                     ELAQELGVSRPTVRAGLHALAAMGVTESRQGAGTFITGGPPRLGAGPLSFLAALHGFT
                     RDEIFEARRILEVGAAGLAAEVNLTDRHATMSEEVTGMFASLSEPQTFLVHDVRFHRA
                     VAAASGNPVLAALVEMISSLVYEQRRMTVERAHDLKESAEMHRRIYQAIREGDADRAR
                     TEMTRHLDMARQAQASEELPAGVLPSRVVGSVA"
     gene            1183..>2588
                     /locus_tag="VNN25_12000"
     CDS             1183..>2588
                     /locus_tag="VNN25_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HXH92294.1"
                     /translation="MTSLSREDAKTRRIVTLILTLLIAASTLAADSAKIIVAQDGSSG
                     FRTIQAALDSIPAGNAENRTILIRNGTYREKVMITASHVALVGEDREKTRIEYAELRR
                     EWRKTHPDDWGAAAINIASGTTDVIIANLTVRNDYGKTHDDHDHQFAIRSMGTANRIA
                     ILHANVIADGGDTLSLWNSESGLSYYDDSYFEGWVDFVCPRGWAYITNSRFFGHNPNA
                     SIWHDGSKDKDQKLVVRHSQFDGVPGIALGRNHRDAQFYLLDSELSANIADHPIYQVP
                     EPDRYIWGERYYYANVHRAAGAFAWFADNLQTAPGSPRDEDITAEWTFGGRWNVEKLP
                     AVLPFAAIPRPENGWPWVDPFGETLHWTPGRDARAHRVYFGATNPPPFRAEQTETTYA
                     TGGLKGGATYFWRVDTVTADGIVPGPVWSFRADTRKVRITLVGDSTMTDKSGWGSGFR
                     RYVAENAALLNLSRGGRSS"
BASE COUNT          488 a          848 c          785 g          467 t
ORIGIN      
        1 cggccagttc caaggccgcg tcgagcgagg cgcgatcgct cacatctgcg acgatccgtg
       61 cggtgcgccg gccgcgcgct gcgacctcat tcgcagcggt atcgacctgc tcgtggcgcc
      121 gcgagatcgg cgcgacgtcc gctccggcgt ccgcgagacc gcgcacgagc gcgaggccga
      181 tccccgacgt tccgcccgtt acgacagcga cgcggccatc gagaggaagc gcgctcaagc
      241 gaccgacccc acgacgcgcg atggcaacac gcccgccggc aactcttccg aagcctgcgc
      301 ctgacgcgcc atatcgaggt gacgcgtcat ctccgtgcgt gcgcgatcgg catcgccttc
      361 gcgaatggct tgatagatac ggcggtgcat ctccgctgat tccttcaagt cgtgcgcacg
      421 ctcgaccgtc atgcggcgct gctcgtaaac gagcgacgag atcatctcta ccaacgcagc
      481 cagcaccggg ttgccggaag ccgcggcaac ggcacgatga aaccggacgt cgtgcacgag
      541 gaacgtctga ggctcgctca ggcttgcgaa catccctgtc acttcctcgg acatcgtggc
      601 gtgccggtcc gtgaggttca cttcagcagc cagtccggca gcgccgactt cgaggatccg
      661 gcgcgcttcg aagatttcgt cgcgggtgaa accgtgaagt gcggcgagga aactcaatgg
      721 gcccgcgccg aggcgcggcg ggccgccggt gatgaaggtg ccggcgccct ggcgcgattc
      781 ggtgacgccc atcgctgcca gcgcgtgcaa tcctgcccgg acagtcgggc ggctcacacc
      841 cagctcttgc gccagctccc gctcaggcac cagacggtcg cccgggcgca gcttgccacc
      901 ttcgatgaga accgcgacgt gtcgcaccac ctgatcggcc gactgaatgt cgatggcgtg
      961 ggctgacggc tcgtccttct ccttcttcac gggcgtgctc ctcgtttcag tgtgtcgtaa
     1021 tggcttgacc aattctctac acctgatcga gattgtcaac cacatggatt gaccaattca
     1081 agatttgcgg agtagcattt gttgctctcg cagcagtttc acggctagga catgtcgaaa
     1141 ttaccgaatt ggtcttacct ctatctttct tcgcctcttc gtgtgaccag tctttcacgc
     1201 gaagacgcga agacgcgaag gatcgtcacc ctgatcctta ctctgctgat cgccgcgagc
     1261 acgttggcgg ctgactcagc aaagatcatc gtcgctcagg atggcagcag cggctttcgc
     1321 accattcagg ctgccctcga cagcattccg gccggcaacg cggaaaaccg aacgatcctc
     1381 atccgcaacg gcacctaccg cgagaaggtg atgatcacgg ccagtcacgt tgcgctggtt
     1441 ggtgaagatc gcgagaagac acggatcgag tacgccgagc tgcggcgcga atggcgcaaa
     1501 acgcatcccg atgactgggg cgcggcagcg atcaacatcg cctccgggac gacggacgtg
     1561 atcatcgcca acctgaccgt ccgcaacgac tacggcaaga cgcacgacga tcacgatcat
     1621 cagttcgcca tccgttcgat gggcactgcc aaccgcatcg ccatcctgca tgcgaacgtg
     1681 atcgcggacg gcggcgacac gctcagtctt tggaacagcg agtcgggcct gtcgtactac
     1741 gacgattcat atttcgaagg ctgggtcgat ttcgtctgtc cgcgcggctg ggcttacatc
     1801 accaacagcc gcttcttcgg ccacaacccg aacgcgagca tctggcacga cggcagtaaa
     1861 gataaggatc agaagctcgt cgtgcgccac tcgcagttcg acggggtgcc cggcatcgcg
     1921 ctcgggcgca atcaccgcga cgcacagttc tatctgctcg actccgagct ctctgccaac
     1981 atcgccgatc accccatcta ccaggttccc gagcccgatc gatacatctg gggcgagcgt
     2041 tactactacg caaacgtcca tcgcgctgcc ggcgcattcg cgtggttcgc tgacaacctc
     2101 cagaccgctc ccggctcgcc gcgggatgag gacatcaccg cggagtggac attcggcgga
     2161 cggtggaacg tcgagaagct gccggcagtt ctgccgttcg ccgcaatccc tcggcccgag
     2221 aacggctggc catgggtcga tccctttggt gaaaccctgc attggacgcc gggccgcgac
     2281 gcgcgagcac atcgcgtcta ctttggtgcg acgaatccac cgccgtttcg cgccgagcaa
     2341 accgagacga cgtatgcaac gggcggactc aaaggcggag cgacctattt ctggcgcgtt
     2401 gacacggtga ccgcggatgg catcgtgcct ggcccggtct ggtcattccg cgccgatact
     2461 cgcaaggtcc gcatcacgct cgttggcgat tcgacgatga cagacaagag cggatggggg
     2521 agtgggttca gacgatatgt cgccgagaat gcggcgttgc tgaatctctc gcgtggcggg
     2581 cggagctc
//
LOCUS       DATLVW010000400         3798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_552181, whole genome shotgun sequence.
ACCESSION   DATLVW010000400 DATLVW010000000
VERSION     DATLVW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552181"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..834)
                     /locus_tag="VNN25_12005"
     CDS             complement(88..834)
                     /locus_tag="VNN25_12005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92295.1"
                     /translation="MSRLTLVVPCYNEERRLDVEAFHQLAVPGHDVDFLFVNDGSRDG
                     TLRLLETLRDSDPRRFAVLDLEQNSGKAEAVRRGFLAAMDKAPDYVGFWDADLATPLA
                     ELPGFLDILTNRSSIHLVMGARVRLLGREISRRPARHYFGRVGATLISSSLGLAVYDT
                     QCGAKVFRSSDTLRDVFSKPFISRWIFDVEIIARYIATWGRDAVAGSVYELPVMRWHD
                     VTGSKVKSHDFLRALADLWKIRRAYRKQKS"
     gene            864..1487
                     /locus_tag="VNN25_12010"
     CDS             864..1487
                     /locus_tag="VNN25_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HXH92296.1"
                     /translation="MTDLEVHTIPATVHGRYVVRRGPAEHLLVGFHGYGENADVHNAE
                     LQQIPGIERWTTVAVQALHPFYMNRTGAIVASWMTSQDRELAIEDNVAYVRSVIDQFP
                     QPRQLVFLGFSQGVAMAYRAAARSAATGLIALGGDMPPELAGSHPTLPPVLIGRGTRE
                     EWYTDEKLQKDLRVLATLTTVETCVFEGGHEWTAEFRDAAGRFLAKL"
     gene            1690..3564
                     /gene="uvrC"
                     /locus_tag="VNN25_12015"
     CDS             1690..3564
                     /gene="uvrC"
                     /locus_tag="VNN25_12015"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXH92297.1"
                     /translation="MTAARGMTIVTQPVPLLRMSERLDRLKLRLKEMPDRPGVYLHKN
                     AEGEVIYVGKARNLKSRVGSYLAGRGARDSKTMSLINEIDSIDFVTTNNELEAILLEN
                     NLIKSNQPRYNILLRDDKSYPYIKVTMSEDYPRVVFTRRLDRKKGDLFYGPFFAGTAR
                     RLLKLVADQFKLRSCDLDITIGKNALPRPCLYYDMHQCLGPCVVGLTTPEQYRDMVDD
                     VVLFLGGKTKELQDRLTNRMYQSAESENYEMATYYRDLVRTVERVQAEQQVASAEDED
                     ADVWGLYEEGSDVAVQLFVIRGGNVVDRRELFWEKVEAYQAPEFLGEVLQRYYQDNMF
                     IPHEVLIPFDVEECDLLTAWLTEQAGRKVGLRVPQRGRGVDLMELVNRNAHLSHESRF
                     RKSTQDRLQIAAQKLGQVLGYDREIGRIESFDISNIQGTDSVAGMVVLDRGKFDKKQY
                     RIFNIKTVEGADDFRSMAEAVDRRYRRLLEENKTLPDMILIDGGRGQLNAALAALNKL
                     GIEEIPIAGLAKREEEIYVPGREEPYRLERRDPALQLLQMVRDETHRFSVSSHRRRRS
                     KRTLHSALDDMPGIGAKRRRLLIERFGSVSGVKQASAQDLVNVLGRKVGQTLYEELHG
                     "
BASE COUNT          746 a         1153 c         1231 g          668 t
ORIGIN      
        1 gacgcaaaga ttttcaccaa aactgattgg ttgcagattt ggtgggtttc atttggttgc
       61 ggctatgccg cgctgtgtct ctgtggttca gcttttctgc ttccgatacg cgcggcgaat
      121 cttccagaga tccgccagcg cgcgaaggaa gtcgtgcgac ttcacctttg acccggtgac
      181 gtcgtgccag cgcatgacgg gtagctcgta gacggaaccg gccaccgcgt cgcggcccca
      241 ggtcgcgatg taacgggcga tgatctcgac gtcgaagatc cagcgggaga tgaacggttt
      301 ggagaagacg tcgcgcaacg tgtcgctgct gcggaagact ttcgcgccgc attgcgtgtc
      361 gtagacggcc aggccgaggc tggaggagat gagcgttgcg ccaacgcggc cgaaatagtg
      421 gcgggccggg cggcggctga tctcgcgccc gaggagacgg acgcgggcgc ccatgaccag
      481 atggattgag gaacggttcg tgaggatgtc gaggaacccc ggcagctccg cgaggggagt
      541 ggcgagatcg gcatcccaga agccaacgta gtcgggcgcc ttatccatcg cggccaggaa
      601 tccgcggcgc acggcctccg ctttgccgct gttctgctcc agatcgagga ccgcgaagcg
      661 acggggatcg gagtcgcgaa gcgtttcgag aagccgaagt gttccatcgc ggctgccatc
      721 gttgacgaag aggaaatcga cgtcgtggcc ggggacggcg agctgatgaa atgcttcgac
      781 atcgagccgt cgctcttcgt tgtagcaggg gacgaccaga gtcaggcgcg acacgcgggg
      841 cgcattgtag ctacgattgg ccaatgaccg acctcgaagt ccacaccatt cccgcaactg
      901 ttcacggacg ctacgtcgtg cgccgcggac ccgcagaaca tcttctggtc ggctttcacg
      961 gttacggcga gaacgccgac gtccacaatg ccgagttgca gcagatcccg gggatcgagc
     1021 gatggacgac ggttgctgtg caggcgctcc atcctttcta tatgaaccgg acgggagcga
     1081 tcgtggcgtc gtggatgacc agccaggatc gggagctggc catcgaagac aacgttgcct
     1141 acgttcgcag cgtcatcgat cagttcccgc aaccgcggca gctcgtgttt ctcgggtttt
     1201 cgcaaggcgt ggccatggcc taccgcgctg ccgcacgttc ggcggcaacg ggactcatcg
     1261 cgctgggcgg cgacatgcct ccggagttgg ctggctcgca tccgactctg ccgcctgtgt
     1321 tgatcggacg cgggacacgc gaggagtggt acaccgacga gaagctgcag aaggatcttc
     1381 gtgtcctcgc tacgctgacc acggtcgaga cctgcgtatt cgaaggcggc cacgagtgga
     1441 ccgcagagtt ccgcgacgca gcggggcggt ttctggcgaa gctctgagtt cggagtgcag
     1501 gcgtctcgcc tgcggccggt gggcgtctcg cccggcggtg tgtggcgagc ggtcgcggtg
     1561 atctgtctga gactcgcagt gatcggcaag gtgacggtca tactcgcgat acacacgccg
     1621 aggcgagacg cctcgacgcc gcggacgaga cgtccgcatg actcgtcaac tacttgacga
     1681 gtcagcggaa tgaccgctgc gcgtgggatg acaatcgtca cgcaacctgt tccacttctg
     1741 cgcatgtccg aacggctcga ccgcctgaaa ctccggctga aggaaatgcc ggaccggccc
     1801 ggcgtctatc tgcacaagaa cgccgagggg gaggtcatct acgtcgggaa ggcgaggaac
     1861 ctgaagagcc gcgtgggctc gtatctggcc ggccgcggag cgcgcgacag caagacgatg
     1921 tcgctcatca acgagatcga ctccatcgac ttcgtgacca cgaacaacga gctggaagcc
     1981 atcctcctcg agaacaacct catcaagtcc aaccagccgc gctacaacat cctccttcgc
     2041 gacgacaaga gctacccgta catcaaggtc acgatgtcgg aggactaccc gcgcgtcgtg
     2101 ttcacccgcc gcctcgatcg gaagaagggg gacctcttct acggcccctt cttcgccggc
     2161 accgcgcggc ggctgctcaa gctcgtcgcc gatcagttca aactccgcag ctgcgatctc
     2221 gatatcacga tcggcaagaa cgcgcttccc cgtccctgcc tctactacga catgcaccaa
     2281 tgcctcgggc catgcgtcgt cggcctgacc actcccgagc agtaccgcga catggtcgac
     2341 gacgtcgtcc ttttcctcgg cggcaaaacc aaagagcttc aggatcgtct aaccaatcgg
     2401 atgtatcagt cagcggagag cgaaaactac gagatggcga cgtactaccg cgatctcgtc
     2461 cgcacggtcg agcgcgtgca ggccgagcag caggtggcct cggccgagga tgaagacgcc
     2521 gacgtctggg gcctttacga ggaggggagt gacgttgcgg tgcagctctt cgtcatacgc
     2581 ggaggaaacg ttgtcgaccg ccgcgagctt ttctgggaga aagtcgaggc ttaccaggct
     2641 cctgagtttc tcggcgaggt tcttcagcgc tactaccagg acaatatgtt catcccgcac
     2701 gaggtactga tcccgttcga tgtcgaggag tgcgaccttc tgaccgcctg gctgaccgag
     2761 caggccggcc ggaaggtcgg actgcgcgtt ccgcagcggg ggcgaggcgt cgatctgatg
     2821 gaactggtca accggaacgc acacctctcg cacgagtcac gcttccgcaa gtcgacccag
     2881 gaccggctgc agatcgctgc acagaaactt gggcaggtgc tcgggtacga ccgggagatc
     2941 ggacgcatcg aatcgttcga catctcgaac atccagggaa ccgactcggt ggcagggatg
     3001 gtcgtcctcg accgcggaaa gttcgacaag aagcaatacc ggatcttcaa catcaagact
     3061 gtggaaggtg cggacgactt ccgctcgatg gctgaggcgg tcgaccggcg ttaccggcgt
     3121 ctgctcgagg agaacaagac gctgccagac atgatcctca tcgatggcgg ccgcggtcag
     3181 ttgaacgctg cactcgcggc gctgaacaag ctcggcatcg aggagatccc gatcgcaggg
     3241 ctggccaagc gcgaggagga gatctacgtc ccgggccgcg aagagccata ccgtctcgag
     3301 cgccgtgatc ccgcgctgca acttctgcag atggttcggg acgagacgca tcgcttttcg
     3361 gtctcctcgc accgccgccg ccgatccaaa cgtacgctgc acagcgcgct cgacgatatg
     3421 cccggcatcg gcgccaaacg ccgccgcctt ctcatcgagc gattcggcag cgtctccgga
     3481 gtcaaacagg catcagcaca ggatctggtg aacgtgctgg gacgcaaggt ggggcagacg
     3541 ttgtatgagg agttgcatgg gtagggtgtg gggagtaggg ggtggggggt gggagctttc
     3601 gcgggagtgg ggagtcgggg gtcgggagtc ggagagattg gcacttggat tcggactact
     3661 tgcccgactc cgaactcgcg aagatcggtg cgagtgggca tcgggcagac tcggctcttt
     3721 ctccgactcc cgacccccga caaccgactc ccgcaaagct cccacccccc actccccaca
     3781 ccccaccccc caaaccag
//
LOCUS       DATLVW010000401         6026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_351274, whole genome shotgun sequence.
ACCESSION   DATLVW010000401 DATLVW010000000
VERSION     DATLVW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351274"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1518)
                     /gene="malQ"
                     /locus_tag="VNN25_12020"
     CDS             complement(10..1518)
                     /gene="malQ"
                     /locus_tag="VNN25_12020"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXH92298.1"
                     /translation="MTHTSPAATKVNNRAAGVLLHPTSLPGAYGIGDLGDELLAFLDW
                     AKKAGLRIWQVLPLNAPGYGNSPYGCLSSYAGNPMLISPKRLLECGLLPSDAIDEIPN
                     PGKIDFDGVAASKNDLLHRSFAHFNENASDEQRSALASFVRDNRWLSDWALYASLKHR
                     HGGAPWTSWPAALVTRDPDAMAAAQKELADEIRFHEYIQWLFFTQWASVREAAHERGI
                     QIMGDVPIYVAADSADVWANREIFQLDENGEPDVVAGVPPDYFSATGQRWGNPLYRWD
                     LLRETNYAWWVARFRASLQFADIVRVDHFRGFAAYWEIPASEPTAIHGRWLPGPGREL
                     FDAIRKAVGGLPIVAEDLGHITPEVHELRKAIGVPGMKILQFAFAQPDSPHLPHHYEP
                     ETVVYTGTHDNDTARGWYEQASNEERTTAALYLGVHTPEELPWTLIRTAYTSVAETAI
                     VPAQDILGLGNEARMNLPGEEKDNWSWRLVEGALTEELAEELRKLALVSGRI"
     gene            complement(1589..4027)
                     /locus_tag="VNN25_12025"
     CDS             complement(1589..4027)
                     /locus_tag="VNN25_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HXH92299.1"
                     /translation="MRIRFICIHGHFYQPPRENPWLEAVETQDSATPYHDWNERVTAE
                     CYGPNASSRILDARDRIVKIVNNYSAISFNFGPTLLSWLEQSDSETYAAIIDADRISR
                     ERFDGHGSAIAQAYNHMIMPLANRRDQQTQTWWGIRDFEYRFGRKPEAMWLPETAVDV
                     ASLEALAAMGIAFTILEPHQALRWRKIGDERWTEHSGAIDPTMPYRCNLPSGRSIDIF
                     FYDGAISRAVAFERLLARGENFAHRLLGAFGEGRSHPQLVNIATDGETYGHHHRNGDM
                     ALAYALDYIDEQKLSRIANYGQFLAATPPTHEVQIADRTAWSCDHSLGRWERDCGCST
                     GGDAGWNQQWRAPLRAALDWLRDECIAIFEMRGAALLRDPWGARDDYIEVVLDRSEDN
                     VERFLARQMKDGDRSAAAKVAVLELMEMQRNAMLMYTSCGWFFNDLSGIETVQVLHYA
                     ARVIQLAEKVSDSSLEPEFLARIASARSNLPHRGDARQIYEREVKPTRVDLRRVAAHY
                     AVASLFDNFAHDDLVYCYRVRQTDFESFRAGRSRMAIGSVIVTSLITHEEASFDFTVI
                     HLGETELTGGVKASTTASDYEAMKTALSEDMRVNGVSAVIRVLDEHFEEESLLSIRSL
                     FRDEQRRILNVLCDATLSEAEGAFRQLHERYDPLTRFHADLGVPLPKVLRTAAEFDVN
                     MQLRRMATASELPLGEMESCLREARDERVVLDETTLMSLTRAVERAAEGVWRDPDDLE
                     LLERWETLVAVIRESAVDVDLRRAQNDYYRLMKTVRPAKATTAGNGSSNANRWLQHFD
                     ALGEKLSISPPGDK"
     gene            complement(4043..>6026)
                     /locus_tag="VNN25_12030"
     CDS             complement(4043..>6026)
                     /locus_tag="VNN25_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HXH92300.1"
                     /translation="VEQQQSSPYSLLWWMKRLIAQRNRYQAFGRGTMEFLHPENRKIL
                     AFVRRYGDEIILIVANLSRLVQCFEVELSGFRGMVPVELSGGTRFPEISDRPYFLNLG
                     PFAFYWFALERQQSDVKVASEEALTIAATTIDEVLSDANRNSLRRALQAYLHTRRWFG
                     GKARTITSLQLRGTVALPDDAGSLAFVHIDYADGEPETYVIPLGIMRARRASEQERAH
                     APAVIARLGDGKLLYEPLADTKFGAALLDTIARRRALKTDHATVSGTPTRIYRTLRGT
                     GELPIQILKTEQSNTALLFGDRFFMKLFRRLEAGVNPELEMTRFLNEETSFTGTPQLA
                     GSIQYEPAVSGEPSTIAVLQRFAPNAGDAWSYTLDSMGRYFEAILSDSGAASQIAKTA
                     SNEPILALTSKPSPDIAHALIGVYLADADVLGRRTAQMHLALASRSDIPAFAPEPITP
                     HYQRSIYQHIRTQIVQTMQLLRKRARGNPDADELLQREEELQKRVRRILDGRLSGLRI
                     RIHGDFHLGQVLKSGNDFIVIDFEGEPSRPLSERRIKRSALRDVAGMLRSFHYASHAL
                     LYGQSQHSVIRAEDSAAAETGAAFWYRWVSAAYLRGYLAESGDAAHLPRTHEELQVLL
                     DVRLIEKALYEISYELNNRPDWVHIPLRGVLELLGR"
BASE COUNT         1114 a         1845 c         1910 g         1157 t
ORIGIN      
        1 cgccggagtc taaattctcc cggagaccag agccaatttc cgcagttcct ccgcaagctc
       61 ctccgtgagc gcgccttcca ccagccgcca cgaccagttg tccttctctt cgccggggag
      121 gttcatgcgg gcttcgttgc ctaggccgag gatgtcctgg gcggggacga tggcggtctc
      181 ggcgaccgag gtgtatgcgg tgcggatcag cgtccagggg agctcctcag gtgtgtgcac
      241 gccgaggtag agcgcggcgg tcgtgcgctc ttcgtttgag gcctgctcgt accagccgcg
      301 ggcggtatcg ttgtcgtgcg tcccggtgta aacgacggtt tccggctcat agtgatgtgg
      361 caaatgcggg ctgtccggct gtgcgaatgc gaactgcagg attttcatcc ccggcactcc
      421 gatcgccttt cttagttcat gcacctcggg tgttatatgg ccgaggtctt cggcgacgat
      481 ggggagcccg ccgacggcct tacgaatcgc gtcgaacagt tcgcggcccg ggccgggcag
      541 ccagcggccg tgaatcgcag tcggttcgct cgcggggatc tcccaatacg cggcgaaccc
      601 gcggaaatga tcgacgcgca cgatgtcggc aaattgcaag gacgcgcgaa atcgcgccac
      661 ccaccacgca tagttcgtct cgcgaagaag atcccagcga tagagcggat ttccccatcg
      721 ctgacccgtc gcgctgaagt aatcgggagg aacgccagcg acgacgtccg gctcgccgtt
      781 ttcgtccaat tggaagatct cgcggttcgc ccagacatcg gcactgtctg ccgcaacata
      841 gattgggaca tcgcccatga tctgaatacc gcgctcatgc gccgcttcgc gtaccgaggc
      901 ccattgagta aagaagagcc attggatgta ttcatgaaaa cggatctcgt ctgccaattc
      961 cttctgcgcc gcagccattg cgtccgggtc ccgcgtaacc agcgcggccg gccacgatgt
     1021 ccagggcgcg ccgccatgac ggtgcttcaa cgatgcatac agtgcccaat ccgatagcca
     1081 tcgattgtca cgaacgaatg aggccagagc gctgcgttgc tcatccgatg cgttttcatt
     1141 gaaatgcgca aacgaccgat ggagcaggtc attcttcgat gcggcaaccc cgtcgaaatc
     1201 gatcttccca ggatttggga tctcatcaat tgcatcggag ggcagcaatc cgcattccag
     1261 cagccgcttt ggcgaaatga gcattggatt tccggcataa gacgaaaggc agccataagg
     1321 cgagttgccg tagccgggag cgttcaatgg caacacctgc cagatgcgca ggcctgcctt
     1381 ctttgcccaa tcgaggaaag cgagcagctc atcgccgagg tcgccgatgc cgtaagctcc
     1441 ggggagcgac gttggatgca gcagcacgcc agcggcgcgg ttgttgactt tcgttgcggc
     1501 gggggaagta tgagtcattt taggaagaga acacagcgtt gtgatgtctc tgtgatctct
     1561 gtgtctttgt ggtgatcggt tttggtcttc atttgtcgcc cggggggctg atcgacagct
     1621 tttcccccaa cgcatcgaaa tgttgcagcc agcgattggc attggaagat ccgttcccag
     1681 cggtcgtcgc cttcgctggg cgcacggttt tcatcagccg gtagtaatcg ttttgcgctc
     1741 gtcggagatc gacgtcgacg gccgactcgc gaatgactgc cacaagcgtc tcccaccgtt
     1801 cgagcagctc caggtcgtcc ggatcgcgcc ataccccctc cgcggcgcgc tcgacggcgc
     1861 gtgtgagcga catcagcgtt gtctcgtcca gcacgacccg ctcgtcgcgt gcttcgcgca
     1921 aacacgactc catctcgcca agcggcagct cactcgcggt ggccatgcgc cgcagttgca
     1981 tattgacgtc gaactcggcc gcggtgcgca acaccttcgg cagcgggacg ccgaggtcag
     2041 cgtgaaaacg cgtgagcggg tcataccgct cgtgcaactg ccggaacgcc ccctcggcct
     2101 cactgagcgt tgcgtcgcag agcacgttga ggatgcgccg ctgctcgtcg cgaaagagcg
     2161 agcggattga taagagcgac tcctcctcga aatgctcgtc gagcacgcgt atgaccgcgg
     2221 aaacgccatt cacgcgcatg tcttcgctga gcgcggtttt catcgcctcg tagtcgctcg
     2281 cggtcgtcga tgccttcacg cctccggtca gctccgtctc gccgaggtgg atgaccgtga
     2341 agtcgaagga agcttcttcg tgcgtgatca gcgacgtcac gatgaccgag ccgatcgcca
     2401 tccgcgaacg gccggcgcgg aacgactcga agtcggtctg gcgcacgcga tagcagtaga
     2461 cgagatcatc gtgcgcgaag ttgtcgaaca gcgatgccac cgcgtagtgc gccgccacac
     2521 ggcgaagatc gacacgcgtc ggtttcacct cgcgctcata gatctggcgt gcgtcgccgc
     2581 ggtgcggcag gttgcttcgg gcgctggcga tgcgcgcgag gaactccggc tccaggctgc
     2641 tgtcggacac tttctccgcc aactgaatga cgcgcgccgc gtagtgcaac acctgcacgg
     2701 tctcgatgcc ggagaggtcg ttgaagaacc agccgcagct cgtgtacatg agcatggcgt
     2761 tccgttgcat ctccatcagt tcgaggacgg ccaccttcgc cgcggcgctc cgatccccat
     2821 ctttcatctg ccgcgccagg aagcgctcga cgttgtcttc cgaccggtcg agcacgactt
     2881 cgatgtaatc gtcgcgcgcg ccccacggat cgcgaagcag cgcagcgccg cgcatctcga
     2941 agatcgcgat gcactcgtcg cgcagccagt cgagcgcagc acgcaacggc gcacgccact
     3001 gttgattcca ccccgcatcg ccgccggtgc tgcagccgca atcgcgctcc catcgcccga
     3061 ggctgtgatc gcagctccag gcggtgcggt cggcgatctg cacctcatgc gtcggaggcg
     3121 tagcggcaag aaactgccca tagttcgcga tgcgcgacag cttctgctcg tcgatgtaat
     3181 cgagcgcgta cgccaaggcc atatcgccgt tgcgatgatg gtggccatag gtttcgccgt
     3241 cggtagcgat gttgacgagc tgcggatgac tgcgcccttc gccgaatgcg ccgaggagac
     3301 gatgcgcgaa gttctcgccg cgtgcgagaa ggcgctcgaa cgccacagca cgcgagatgg
     3361 cgccgtcgta gaaaaagata tcgatggaac gccccgacgg caggttgcag cgatacggca
     3421 tcgtcggatc gatcgctccg gagtgctcgg tccaacgctc gtccccgatc ttccgccagc
     3481 gcagggcctg atgcggttcg aggatcgtga acgcgatgcc catcgcggcc agcgcctcga
     3541 gtgaggccac atcgaccgcc gtttccggca accacatcgc ctccggtttg cgcccgaagc
     3601 ggtactcgaa atcgcggatc ccccaccacg tctgcgtctg ctgatcgcgc cggttcgcga
     3661 gcggcatgat catgtggttg tacgcctgcg cgattgcgga gccgtggccg tcgaatcgct
     3721 cgcggctgat gcgatcggcg tcgatgatcg cggcgtacgt ctccgaatcg ctttgctcga
     3781 gccaggagag cagcgtcggc ccgaagttga aactgatcgc cgagtagttg ttgacgatct
     3841 tgacgatgcg atctctggcg tcgaggatcc gcgacgaggc gttcgggccg tagcactcgg
     3901 cggtgacccg ctcgttccag tcgtggtacg gcgtggcgga gtcctgcgtc tccaccgcct
     3961 cgagccatgg gttctcgcgc ggcggctgat agaaatgtcc atgaatgcag atgaaacgga
     4021 tgcgcatcgg gagcggagaa tatcagcgcc cgagaagctc caacaccccg cgcagaggga
     4081 tgtggaccca gtccgggcgg ttgttcaatt cgtagctgat ctcatacagc gctttctcga
     4141 tcaggcggac gtcgagcagg acctgcagtt cctcgtgcgt gcgaggcaag tgagcggcat
     4201 cgccgctttc ggccagatag ccgcggaggt acgcggcgct cacccagcga taccagaagg
     4261 ccgcgcctgt ttccgccgcg gcggaatcct ccgcgcggat gacggagtgc tgcgattggc
     4321 cgtacaacaa cgcatgcgag gcgtaatgaa acgatcgcag catgccggcg acatcgcgca
     4381 gcgcgctgcg tttgatgcgg cgctcgctca gcggacggga tggctcgcct tcgaaatcga
     4441 tgacgatgaa gtcgttgccg ctcttcagaa cctgcccgag gtggaagtcg ccgtggatgc
     4501 gtatgcgaag accgctcagc cgaccgtcga gaatgcggcg tacacgtttc tgcagttcct
     4561 cttcacgttg caacaattcg tcagcgtccg gattccctct tgcgcgtttg cgcaggagct
     4621 gcatggtttg cacgatctgc gtgcggatgt gctgatagat cgagcgttga tagtgcggcg
     4681 tgatcggctc cggagcaaag gcaggaatgt cgctgcgcga cgccagagcc agatgcattt
     4741 gcgccgtgcg cctcccaagc acgtcggcat ccgccagata gacgccgatc agagcatgag
     4801 cgatgtcggg agacggcttc gaagtgagag cgaggatcgg ctcgtttgag gccgtcttcg
     4861 cgatctgcga tgccgcgccg gagtcgctga gaatggcttc gaaatagcgt cccatggaat
     4921 cgagcgtgta actccaggcg tcgccggcgt tcggtgcgaa gcgctgcagg acagcaatcg
     4981 tggacggctc gccgctgacc gccggctcat actgaatcga gccggcaagc tgcggcgtgc
     5041 ccgtgaagga tgtctcctca ttgaggaacc gcgtcatctc gagctcgggg ttgacgccgg
     5101 cctcgaggcg ccggaagagt ttcatgaaga aacggtcgcc aaagaggagc gcagtattgc
     5161 tttgctctgt cttcagaatc tgaatcggga gctcgccggt ccctcgcagg gtgcggtata
     5221 tgcgcgtcgg cgtgcccgaa acggttgcgt ggtcggtctt gagcgcgcgg cggcgggcaa
     5281 tggtgtcgag cagcgcggct ccgaacttcg tgtctgcaag tggctcatac agcaacttgc
     5341 catcaccgag gcgggcgatt acagcgggtg cgtgtgcgcg ctcctgctcg ctcgcgcgtc
     5401 gagcccgcat gataccgagc ggaatcacgt acgtctccgg ctcgccgtcc gcatagtcga
     5461 tgtggacaaa tgcgagagaa cccgcgtcgt cggggagcgc gacagtcccg cgcagttgca
     5521 acgaagtgat cgtccgcgct tttccgccaa accagcggcg ggtatgcaaa tacgcctgca
     5581 gcgcgcgtcg aagcgaattc cgattcgcgt cggagaggac ttcgtcgatt gtggttgctg
     5641 caatcgtaag tgcttcttcg ctagccacct taacgtcgga ctgctggcgc tccagggcaa
     5701 accagtaaaa ggcgaacggc ccgaggttga ggaaataggg gcgatcactg atttccggga
     5761 agcgcgttcc gcccgacagc tcgacaggca ccatgccgcg gaaaccggaa agttcgacct
     5821 cgaagcactg gaccagccgc gagagattgg cgacgatgag aatgatctcg tctccatacc
     5881 gccgcacgaa ggcgaggatc ttgcggttct ccgggtgcag aaactccatg gttccgcggc
     5941 caaacgcctg gtatcgattg cgctgcgcga tcagtcgctt catccaccac agcagcgaat
     6001 aggggctcga ctgctgctgc tcgacg
//
LOCUS       DATLVW010000402         5286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_61239, whole genome shotgun sequence.
ACCESSION   DATLVW010000402 DATLVW010000000
VERSION     DATLVW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61239"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VNN25_12035"
     CDS             complement(<1..520)
                     /locus_tag="VNN25_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH92301.1"
                     /translation="MTGNDRKTIGRIVATSLAFGFGAALVLVLVPFGGASENAITGAA
                     LLGFGAGWGLLAFRAARHGQPQRWAAVAAIAFLSFAVCLFLWPRVVASAWFWPSWLFF
                     LIVLAISIAIGACRRLNNWTRPWLVYPIAGVMLAAAFGGLYEAARESSDRTPMRGQLV
                     DIGNRRLHLRCSG"
     gene            complement(836..2908)
                     /locus_tag="VNN25_12040"
     CDS             complement(836..2908)
                     /locus_tag="VNN25_12040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HXH92302.1"
                     /translation="MRRLDRTITAIALSLFATTIAFAEDAKPAAPAAPIAAQRYGTWG
                     VDLDGMDRSVKPGDDFFKYVNGKWAATTQIPADKTGYGAFAVLGDLSEARVHALLDRW
                     AADKSLKPGSDEAKVAAVYRTYLDEATAEKLDAKPLQPYLDAVKKAQTRDDIARIMGR
                     SERGFGSSFFGARIADDAKNPDKYALYVGQAGLGLADREFYLRDKFKPQKERYQKYVA
                     DMLGLIGWADPEKSAADVVALETKIAEAHWTRAESRDRDKTYNPTTIAELEKNAPGFP
                     WLAAFKEGGLDKVDRAVVAQNTAIPKIAKIFGDTPVATLQAWEAFHVADDAAPLLSKR
                     FVDTNWEFRSKFLNGTTEQRPRWKRAVAAAERTMGEAIGRTYVAEYFPPESKAKMEQL
                     VAGLRASMKGRIENVQWMSPETKAKALDKLAKFNVKIGYPSKWRDYSKLEIRDGDMVG
                     NAERAAKFEWARDLSRIGQSVDKQEWGMTPQTVNAYYQSTKNEVVFPAAILQPPFFDP
                     KADMAVNYGGIGAVIGHEITHGFDDQGRKSDGTGMLTDWWAPEDAAKFEVQAAKYGAQ
                     FEAVNFPQLPGIHIIGKLTMGENIGDLGGILMALDAYHLSLHGQPAPVLDGFTGDQRV
                     FLGWAQVWRTLTRDDALRQLITTNPHSPGQVRAFAPLRNVDAWYAAFDVKDGDANYIK
                     PEDRVRIW"
     gene            complement(3006..4001)
                     /locus_tag="VNN25_12045"
     CDS             complement(3006..4001)
                     /locus_tag="VNN25_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH92303.1"
                     /translation="MTNSRSLGSLEVFPIALGCMGMSGMYGASDENESVATIQQAIDS
                     GINLIDTGDFYGMGHNEMLIGRAIRGRRDEVLLSVKFGALRGPDSAWLGVDARPAAVK
                     NFLAYTLKRLNVDYIDIYRPARLDPNVPIEETVGAIAELVKAGYVRGIGLSEVGVETI
                     KRAHAVHPITDLQIEYSLVSRGPERDIFPLLRQLGIGVTAYGVLSRGLLSGSLPQGKS
                     DFRAHMPRFRDENLARNQKLIDALRDIAAAHNVTRVQLAIAWVMARGETIVPVIGART
                     RAQLAESLGALTIRLSSDELTAIEQAVPADGVAGTRYDERQMSVLDSERPPNTPR"
     gene            complement(4120..>5286)
                     /locus_tag="VNN25_12050"
     CDS             complement(4120..>5286)
                     /locus_tag="VNN25_12050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXH92304.1"
                     /translation="DVGSGKTIVALIAAIIMVRNGHQVALLAPTEILVEQHYQRIRQL
                     VDQSLVVAKSTGSLTAGERRTLLAGLREGVIQLVIGTHALLEDRVVFKSLGLAIVDEQ
                     HRFGVEQRQRLFAKGDTPDILVMTATPIPRSLALAMYGDLELSVIDELPPGRQKIKTA
                     VRSATQMERVYHFIDTEIAAGAQVYVVYPIIEESEKSNLKPLTLGFEATAKRFPNRRV
                     AMIHGRMPPAEKEETMQRFKRGEIDILVSTTVIEVGIDVANASLMLIIDADRFGLSQL
                     HQLRGRVGRGARQSYCVLVSDIEKDRLTQFAKTLNGFDLAELDLRIRGAGELLGTRQS
                     GAMRFRFGNIVRDIKLMEKARDLAIEILAKDGLAPAEKIVHKLLGTPMSATAKD"
BASE COUNT          950 a         1638 c         1688 g         1010 t
ORIGIN      
        1 aaccgctgca gcggagatga aggcggcgat ttccgatgtc gacgagctga ccgcgcatgg
       61 gtgttcgatc actcgactca cgtgccgctt cgtaaagtcc gccgaaggcg gcggccaaca
      121 tcacgcctgc gatcggatag accagccacg ggcgagtcca gttgttcagg cgccggcaag
      181 cgccgattgc gatcgagatt gcgagaacaa tgagaaagaa cagccacgag ggccaaaacc
      241 atgcgctcgc gacgacgcgc ggccagagaa acaaacaaac ggcaaaggat aggaaagcga
      301 tcgccgcaac cgccgcccag cgttgcggtt gcccgtgcct cgccgcacgg aacgcgagga
      361 gcccccaccc ggctccaaaa ccaagaagcg cggcgccggt gatcgcattc tccgacgcac
      421 cgccgaacgg cacgaggacc agcaccagcg cagcgccgaa gccgaaggca agagatgttg
      481 cgacgatgcg gccaattgtc ttcctgtcat ttccagtcac ttgaactttg acctttcacg
      541 acgccgcgaa cttcaagctc gtcagtgacc accaagtcat gagggcgcct tctagtgggg
      601 ctgagcagta tacgatctcc ctcaaatgcc gcggtcagca tccacgcgag cagagagaat
      661 tccgagcgaa tacgctcgag accgtacgac tccgtcgcgg taggagcgca cgtgttctcc
      721 ggcggcgcaa cattgcgtcg cgataggagt gcacgcgcgc acccgcgatg ccacgctgcg
      781 acacgaaaaa cggccacctt gcggtggccg ttctgcttga tgcgatcctt gtcgcttacc
      841 agatccgaac gcgatcctcg ggtttgatgt agttcgcgtc gccgtccttt acgtcgaacg
      901 cggcgtacca ggcgtcgaca ttgcggagag gggcgaaggc gcgcacctgg ccgggggaat
      961 gcgggttcgt ggtgatgagc tggcgtagcg cgtcgtcgcg ggtcagtgtg cgccatacct
     1021 gcgcccatcc caggaacacg cgctgatcgc cggtgaagcc gtccagcact ggcgcgggct
     1081 ggccgtgcag cgagagatgg taggcgtcga gagccatgag gatgccgccg aggtcgccga
     1141 tgttctcgcc catcgtcagc ttgccgatga tgtggattcc ggggagctgc gggaagttga
     1201 cggcttcgaa ctgcgcgccg tacttcgcgg cctgcacctc gaacttcgcc gcatcttcgg
     1261 gcgcccacca gtcggtcagc attccggtgc cgtccgactt gcggccttga tcgtcgaagc
     1321 cgtgcgtgat ctcgtgcccg atcacggcgc cgatgccgcc gtagttcacg gccatgtccg
     1381 ctttcggatc gaagaacggc ggctgcagaa tcgccgcggg gaagacgacc tcattcttcg
     1441 tcgactggta gtaggcattg accgtttgcg gcgtcatgcc ccactcctgc ttatcgacgg
     1501 actggccgat gcggctcaga tcacgcgccc actcgaactt cgcggcgcgc tctgcgtttc
     1561 cgaccatgtc gccgtcgcga atctcgagct tcgaatagtc ccgccacttc gacgggtaac
     1621 cgattttcac gttgaacttg gccagcttgt cgagcgcctt ggccttcgtc tccgggctca
     1681 tccactgcac gttctcgatg cgtcccttca tcgacgcgcg gaggccggcg acgagttgct
     1741 ccatcttcgc cttcgactcc ggcgggaagt actcggccac ataggtccgg ccgatcgctt
     1801 cacccatcgt ccgctcggcg gccgcgacgg cgcgcttcca gcgaggacgc tgttccgtcg
     1861 taccgttcag gaacttcgag cgaaactccc aattcgtatc gacgaaacgc ttcgaaagga
     1921 gcggcgccgc gtcatcggcg acatggaacg cttcccatgc ctgaagagtc gcgaccggcg
     1981 tatcgccaaa gatcttcgcg atcttcggaa tcgcggtgtt ctgcgccacg acggcgcgat
     2041 cgactttgtc gagtccccct tctttgaatg ccgcgagcca cgggaatccc ggagcgttct
     2101 tctccagctc ggcgatggtg gtcgggttgt aggtcttgtc gcgatcgcgg ctttcggcgc
     2161 gagtccaatg cgcctcggcg atcttggtct ccagcgcgac gacgtctgcc gcgctcttct
     2221 cgggatcggc ccatccgatg agtcccagca tgtcggcgac gtacttctga taacgctctt
     2281 tctgcggttt gaacttgtcg cgcaggtaga actcgcggtc ggccaagccg aggccagcct
     2341 gcccgacgta gagcgcgtac ttgtcgggat tcttggcgtc gtcggcgata cgggcgccga
     2401 agaacgagct gccgaaaccc ctttcggagc ggcccatgat ccgggcgatg tcgtcccgcg
     2461 tctgcgcctt tttgaccgcg tcgagatacg gctggagcgg cttggcgtcg agcttttccg
     2521 cggtggcttc atcgagatac gtgcgataga cggcggcgac cttggcctca tcggagcccg
     2581 gcttcagact cttgtcggcg gcccagcggt cgagcagcgc gtgaacccgc gcctcggaaa
     2641 gatcgccgag gacggcgaag gcgccgtatc cggtcttgtc ggccggaatc tgggtcgtgg
     2701 cggcccactt gccgttgacg tacttgaaga aatcgtcgcc cggtttgacc gaacgatcca
     2761 tgccgtcaag gtcgacgccc caggttccgt accgctgcgc tgcgatgggc gctgcgggcg
     2821 cggccggctt cgcgtcttcg gcgaaagcga ttgttgttgc gaacagacta agtgcgatcg
     2881 cagtgatcgt gcgatcaaga cgtctcatgc tcatgcgaat tgtcccttca ggttggatgg
     2941 agcggtttgt ggaactgcca gagtaacaaa atggcaagcg aaagacgtcc agtgacacag
     3001 ttcgttcacc gaggggtgtt cggtggccgc tcactgtcga gcacgctcat ctggcgttcg
     3061 tcgtagcggg tgccggcgac gccatccgcg gggacagctt gctcgatcgc ggtcagttca
     3121 tcgctgctca aacggatggt gagtgcgccg agagactctg cgagctgcgc gcgcgtgcgg
     3181 gcgccgatga cgggcacgat cgtctcgccg cgggccatca cccaggcaat cgcgagttgc
     3241 acccgggtca cgttgtgcgc cgcggcgatg tcacggagag catcgatgag cttctggttg
     3301 cgggcgagat tctcgtcgcg gaagcgcggc atgtgagcgc ggaagtcgct ctttccctgc
     3361 ggcaacgatc cgctcagcaa tccgcgagag agcacgccgt aggccgtaac gccaatcccg
     3421 agctggcgca gcagcggaaa gatgtcccgc tccggaccgc ggctgacgag cgagtactcg
     3481 atctggaggt ctgtgatcgg atggaccgcg tgcgcgcgct taatcgtctc gacgccgact
     3541 tccgacaggc cgatgccgcg gacatagccg gctttcacca gttcagcgat cgcaccgacg
     3601 gtctcctcaa tcggtacgtt cggatcgagt cgagcaggcc ggtagatgtc gatgtaatca
     3661 acgttgaggc gcttcaacgt gtacgccagg aagttcttga cggcggccgg acgggcgtcg
     3721 acacccagcc acgcagagtc cggcccgcgg agcgcgccga acttgacgga aagcagcacc
     3781 tcatcgcgtc gtcctcggat tgcacggccg atgagcatct cgttgtggcc catcccatag
     3841 aagtcgccgg tgtcgatcag gttgatgccg ctgtcgatgg cctgctggat cgtagcgacg
     3901 ctctcgttct cgtctgaagc gccgtacatc ccggacatgc ccatgcatcc gagagcgatt
     3961 gggaagactt cgagcgatcc gagggagcgg gagtttgtca tcgggcaaac ataggacgga
     4021 tcaataggcg tgacaaggac gattcaaagc tttcacgcga agacgcgaag acgcgaaggt
     4081 cttcttcgcg ttcttcgcgc cttcgcgtga aatgcttttc taatccttcg ccgtggcgga
     4141 cattggcgtg ccgaggagct tgtggacgat cttttcggcg ggggccaggc cgtccttcgc
     4201 gagaatctcg atggccaggt cgcgcgcttt ttccatcagc ttgatgtcgc ggacgatgtt
     4261 gccgaagcgg aagcgcattg cgccggactg acgggtgccg aggagctcgc cggcgccgcg
     4321 gatgcggagg tcgagctcgg cgagatcgaa gccgttcagc gttttcgcga actgggtgag
     4381 gcgatccttt tcgatgtccg acacgagcac gcaatacgac tgccgcgccc ctcggcccac
     4441 gcggcctcgg agctggtgca actgagaaag gccgaaacgg tccgcatcga tgatcagcat
     4501 cagcgacgcg ttggcgacgt cgatgccgac ttcgatcaca gtcgtcgaga cgaggatgtc
     4561 gatctcgccg cgcttgaatc gctgcatcgt ctcttccttc tcggccggcg gcatgcgtcc
     4621 gtggatcatg gccacgcgtc tgttcgggaa gcgcttcgcc gttgcctcga agccgagggt
     4681 gagcggcttg agattcgact tctccgactc ctcgatgatc ggatagacga cgtagacctg
     4741 cgccccggcg gcgatctcgg tatcgatgaa gtgatagacg cgctccatct gcgtggccga
     4801 acgcacggcg gtcttgatct tctgccgtcc cggcggcaac tcgtcgatga cggagagctc
     4861 gagatcgccg tacatcgcca gcgccaacga gcgtgggatc ggggtcgcgg tcatcaccag
     4921 gatgtcgggc gtgtctcctt tcgcgaaaag ccgctgccgc tgctcgacgc cgaagcgatg
     4981 ctgctcgtcg acgatggcca gcccaaggga tttgaagacg acgcgatctt cgagcaacgc
     5041 gtgcgtaccg atgacgagtt ggatgacgcc ctcgcgaagg ccggccaaaa gcgtgcgccg
     5101 ctctcccgcc gtcagcgagc cggtgctctt ggcgacgacg agtgactgat caactagttg
     5161 acgaatgcgt tggtagtgct gctcgaccag gatctccgtc ggcgccaaca atgcgacctg
     5221 gtgaccattg cgcaccatga tgatcgcagc gatcaacgcg acgatcgtct tgccgctgcc
     5281 gacgtc
//
LOCUS       DATLVW010000403         9095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_351336, whole genome shotgun sequence.
ACCESSION   DATLVW010000403 DATLVW010000000
VERSION     DATLVW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351336"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VNN25_12055"
     CDS             complement(<1..545)
                     /locus_tag="VNN25_12055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH92305.1"
                     /translation="MQYRELGKTGIKVSAIGFGGWAIGGPSEASGAPLGWGRVSDEES
                     LAAIRRARDLGVTFFDTSDSYGFGRSESLLGIVLSRKRQDAVIATKVGVVRGASGELK
                     KDFSGQHIFAAVDGSLKRLRSDYIDLYQVHNPTLDELRREEIQDAMERLQDAGKIRYW
                     GVSVSSPEEGIEIIRRGWGHAL"
     gene            complement(563..1030)
                     /locus_tag="VNN25_12060"
     CDS             complement(563..1030)
                     /locus_tag="VNN25_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92306.1"
                     /translation="MRSIALLLVGCLLSSCATMTRGRDEVISVDSDPSGANVTILCAG
                     NISAAGTTPARLTIPRKADLCHVDVEKSGMAAQKIQIERGFNASYWMNFVPTLGFPIY
                     VLSSGIWGGNSGTAVGWLAFGLAGGAGLLVDRITGAMYDHDPKVIKVTLQPQH"
     gene            complement(1030..2604)
                     /gene="serA"
                     /locus_tag="VNN25_12065"
     CDS             complement(1030..2604)
                     /gene="serA"
                     /locus_tag="VNN25_12065"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXH92307.1"
                     /translation="MKILVTDTLADSGLAILRAASDMEVDYRPGLKDGPLLAAVAESD
                     ALITRSGTAVTEELVNAGSRLRIVGRAGVGLDNVDVDACTARGILVINAPTANIMSAT
                     EHTMAMLLALCRNIPEAHASVKRGEWNRSKFLGTELDGKILGIIGLGRIGTRITVRAR
                     AFGMRVIAYDPYIAPSVFEKVGAERVSLDDLLTRADVITVHTPLTDETRGMIGAAEMA
                     KMKDGVVVLNIARGGIYEEQALADALNNGKIAGAAVDVYVEEPPKGNPLLAAKNIILS
                     PHIGANTIEAQDRVAVQTAEMVVDALRGSIFVSAVNLPFEGLADADAAPFINLAEKLG
                     LFAAQIISGPITRAAVELWGIDERLTKILSVAGLKGMLTPRLDQSVNFVNAEQVAQQR
                     GIAIAATTHPPPADYTNLVTFRASSAAEEICVSATLFSEKNQRIVSVNNFRVEFKPEG
                     ALLYIINKDVPGVVGKVGTILGDREINIAEYNLARETSGGKALAIVTIDTALDADTMN
                     ALRFFKAIEEVKQVRL"
     gene            complement(2601..3686)
                     /locus_tag="VNN25_12070"
     CDS             complement(2601..3686)
                     /locus_tag="VNN25_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXH92308.1"
                     /translation="MSNSAERATYFVPGPTWVRPAILNEMTRPMIGHRSSEFKELFVR
                     IIANLKPLFGTTQDTLVATCSGTAILEAALINCVPRRLLATTCGAFSERWRAIGESLG
                     LEVDQLNAGWGNAVEPEALADHLTSRRGRYDAVTITHNETSTGVTNDVAALARVVREH
                     SPDTLVLVDAVSSLGGIPVRFDEWGLDVCLASVQKGIALPPGITLFAVSAHALARAAK
                     KPYRGTYFDFLNYKKHADDGSVPSTPSIPHFYALAKQLDDIINVETLDARFPRHEAMR
                     EATIRRTSAYATLASDAAHASATVSALLPNLPAEQIREQMKARGFTLGGGYGKWKNST
                     FRIGHMGDINLDDLNAMLDVLSEVAGS"
     gene            complement(3683..4312)
                     /locus_tag="VNN25_12075"
     CDS             complement(3683..4312)
                     /locus_tag="VNN25_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HXH92309.1"
                     /translation="MTYRLIFFDVDSTLVTIEGIDVLANGAPEIAALTDAAMNGLIPL
                     DQVYARRLDIIKPTRDDVAKLGKTYVNSIVDGVQETIVALQDGGARVHLITGGIEQAI
                     LPLAETLGIPERNVHAVRLRFGASGDYEDFDRRSFLARPGGKELVVRDMRARSHGKAA
                     FVGDGVSDLEAKDAVDLFIGFGGVVVRPLVKENAHKYVTDFRDILQHLS"
     gene            complement(4309..5019)
                     /locus_tag="VNN25_12080"
     CDS             complement(4309..5019)
                     /locus_tag="VNN25_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXH92310.1"
                     /translation="MTLPPLRYRAVLFDLDGTLVDSYAALTEAVNFARREHGLDELSI
                     AHIRGSVGDGLDMLLRRSFDTDDVPRSAHDAFETRYDEICCEKSTILADVEATLKTLH
                     GFGVVMAVCTNKRTSLSKKILTFLQLAPYFSAIVGPDLAGASKPDAKHVLHALETTRC
                     AVADALFIGDMPIDVRAARNSGMDVAVIATGSSTTAELTAAQPDFVLEKFSDLVNLVG
                     REGCQIEVCDDARVSGQP"
     gene            5073..6848
                     /locus_tag="VNN25_12085"
     CDS             5073..6848
                     /locus_tag="VNN25_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH92311.1"
                     /translation="MKHALVALLFFAVPLFAADGKLTVALEPLGDSAEGVVARVSFRF
                     TVPTDAPSGVPLELTGSISQNGQVVKRFRYPVLPSTTSPITAIQTLQPGAAEIDVRLM
                     VPLEEQTPVILAKATQTFTVAKTGTAYVAHEGDAAEAILAEGVVPEGIGAVKIVPPRR
                     DVAPNLFVVEVEVMPPVTRVEWWVEGKKILARNAPPYRAELDLGALPKRVEVRAIGYD
                     AQGRYVDADAFVVNEHETPLEVKITRTVTPDGVSHFKLSVQNPKATVIKSAEFFAGQK
                     KLHAWSGPPYALDVPAAALKGVEFVRASVLDETNYEASDLLFLNGARFSEEIEVNLIE
                     LPVMVVDHAGVPVTDLKQTDFTILENGKPQKITTFNYASNLPIAAGVLIDHSGSMKPR
                     MAQAKTAALEFFKKIMKGGDRAFATGFAFDAVSSAPFVTDVGSLEQQVNAIPDASGGT
                     SLYDAIVTGLYRFRTLQGRKALIILTDGEDTTSRTSYDDMLTYARSARVPLYFIGVGL
                     GLFDASGISKMKTLAAETGGIAYFIHDIKQLGETYTQLEKDLRSQYLIAYNTQSTGKD
                     KAYRKVDVTVSRPNTTVRTIRGFIP"
     gene            6878..7399
                     /locus_tag="VNN25_12090"
     CDS             6878..7399
                     /locus_tag="VNN25_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH92312.1"
                     /translation="MLALACSPAPRPRDRHGNPVDVDLRGAPVTLLHFWAAWCGPCRE
                     ELPRFMAFAATHHLRVVAVSHDRDFTSADRFLRQQGIPLERYWTITAASPPGTTSASF
                     QRQSRSTPTQDHRPIRSVGGLERSGGRTKSAEVIKPEIRDERSETRRFPDLLSLISRH
                     CGIARRDKSEIKR"
     gene            complement(7449..>9095)
                     /locus_tag="VNN25_12095"
     CDS             complement(7449..>9095)
                     /locus_tag="VNN25_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH92313.1"
                     /translation="MARAIAGLPAEAPIKLTDGERLERAVSLMRDGDPQHAFDELDAL
                     QTAGPPPDLRSPVQLNRGLALNQLHRYDESNKALDPLASGAYRFAIPAIFTASQNYLA
                     LASSINPIVTKSVVVKQQVGTITLKSGKKRPKFKNVKKNIQLVDLAKKAKRDEYERLA
                     TERLKDLLTLPLADEVRITVLNRLIAAAEAKNQDPYEQELIVQLEKVDPSQDAGLQHF
                     WDKAWAAYERGDLNGAIPLMAFVRDGYRSPNVKRQAEYWSARCDDRLGRKAAAAATYQ
                     KLAAAPYDDIYAIESEKRGAKRPETNVNPLTSNRPDWRDTAEQTMPQALRLGYELTAL
                     TDFRDGRLEIDRNRNRANQQFADALLAEIYHASGDTESTLKTLRSAFPALATVEQDSV
                     PSYFLRMYYPLKYDDWIRGYSRKNDLDPFTVMGLIHQESYFNPKAKSNVGAMGLMQLM
                     PATGKELAARFHIAPRLDNPETNIRLGAAHFKGLVDLFGNDELAIASYNAGQGRVQQW
                     RRAAPSKSMDEFIESIPFRETRTYVKHVVMIASSYRRMYP"
BASE COUNT         1696 a         2753 c         2841 g         1805 t
ORIGIN      
        1 agcgcgtgac cccatccacg cctaatgatc tcgattccct cctcgggact cgagaccgac
       61 actccccagt agcggatctt gcccgcatcc tgcagccgct ccatcgcgtc ctgaatctct
      121 tcgcggcgca gctcgtcgag cgtgggattg tgaacttgat acagatcgat gtaatcgctg
      181 cggagccgtt tgagcgaacc atcgacggct gcgaagatgt gctggcctga gaaatctttc
      241 ttcaactcgc cgctcgctcc gcgaacgacg ccgaccttcg tggcgattac ggcgtcctga
      301 cgtttgcgcg aaaggacgat gccgaggagc gactcgctgc ggccgaagcc atagctgtcg
      361 gacgtgtcga agaatgtgac gccaaggtcg cgggcgcggc ggatagcagc aagcgactcc
      421 tcatcgctca cgcgccccca cccaagcggc gctccagatg cctcggacgg accgccaatg
      481 gcccagccac cgaagccgat tgcgctgact ttgattcctg tctttccaag ctcgcggtac
      541 tgcatcgcgt acgattctaa ggtcagtgct gcggttggag tgtgactttg atgacctttg
      601 gatcatggtc gtacatcgcg ccggtgatgc gatcgacgag tagaccggcc ccaccggcaa
      661 gaccaaaggc cagccagccg acagcagtcc cgctgttgcc gccccagatt ccactggaga
      721 gcacgtagat cgggaatcca agtgtcggta cgaagttcat ccaatacgat gcgttgaacc
      781 cgcgctcgat ctgaattttc tgcgccgcca tgccgctctt ttcaacgtcg acgtggcaga
      841 gatctgcctt gcgcggaatc gtcaggcgcg ccggcgtggt gccggctgcg gaaatgttgc
      901 ctgcgcagag gatggtgacg ttcgctccgg acggatcgga atcgacggaa atgacttcgt
      961 cgcggccccg cgtcatcgtc gcgcaagagc tgagaaggca accgaccagc aacagcgcaa
     1021 tcgaacgcat cacaacctca cttgcttcac ttcctcaatg gccttgaaga accgcaatgc
     1081 attcatcgta tcggcgtcga gcgcagtgtc gatggtaaca atcgccaacg ctttgccgcc
     1141 gctcgtttcg cgcgcaaggt tgtattcggc gatgttgatc tcccggtcgc cgaggatcgt
     1201 tcccactttg ccgacgacgc ccggaacgtc cttgttgatg atgtagagca acgctccctc
     1261 gggcttgaac tcgacgcgga agttgttgac cgagacgatg cgctgattct tttcgctgaa
     1321 gagtgtggcc gagacgcaga tttcctccgc tgccgacgac gcgcggaagg tgacaagatt
     1381 cgtgtaatcg gcgggaggcg gatgtgtggt cgctgcgatg gcaatgccgc gttgttgcgc
     1441 gacctgctcg gcgttgacga agttcaccga ctgatcgagg cgcggcgtca gcattccctt
     1501 caagcctgca acggaaagga ttttcgtcag acgctcgtcg atgccccaca actcgacggc
     1561 agcgcgcgtg atcgggccgc tgatgatctg cgctgcgaac agtccgagct tctccgcaag
     1621 attgatgaac ggcgcggcat cggcgtcggc cagcccttcg aacggcaggt tgacggcgga
     1681 gacgaagatc gagccgcgaa gcgcgtcgac gaccatctcc gcggtttgca cggcaacgcg
     1741 gtcctgcgct tcgatcgtgt tcgcgccgat gtgcggggaa aggatgatgt tcttcgccgc
     1801 cagcaacgga ttgcccttgg gcggctcttc gacatagacg tcgactgcgg cgccggcgat
     1861 cttgccgttg ttgagggcgt cagcgagtgc ctgctcttcg tagatcccgc cgcgagcgat
     1921 gttgaggacc acgacgccgt ccttcatctt cgccatctca gcggcgccga tcatgccgcg
     1981 cgtctcatcg gtgagcggcg tgtggacggt gatcacgtcg gcgcgcgtga ggagatcatc
     2041 gagcgagacc cgctcggcgc cgaccttctc gaacacgctc ggcgcgatgt aaggatcgta
     2101 agcgatgacg cgcatgccga aggcgcgggc gcgaacggtg atgcgcgtgc cgatgcgccc
     2161 cagtccgatg atgccgagga tcttgccatc gagctcggtg cccaggaact tcgagcggtt
     2221 ccactcgccg cgcttcacgc tggcatgcgc ctccggaatg ttgcggcaaa gcgcgagcag
     2281 catcgccatc gtatgctcgg tggccgacat gatgttcgca gtcggcgcgt tgatgacgag
     2341 gatgccgcgc gcggtgcatg catcgacgtc gacgttatcg agtccgacgc cggcgcgccc
     2401 gacgatgcgc agcctcgacc cggcgttcac caactcctcg gttaccgcag tgccgcttcg
     2461 ggtgatgagt gcgtcggact cggcgacggc cgcgagcaaa gggccatctt tgagacccgg
     2521 ccggtagtcg acttccatgt cgctggcggc gcgaagaatg gccaggcctg agtcggccag
     2581 cgtgtcggtc accaggatct tcacgatcca gcaacctcgg acagcacatc gagcatcgcg
     2641 ttgaggtcgt cgaggttgat gtcgcccata tggccgatcc ggaaggtgct gttcttccac
     2701 tttccgtagc cacctccgag tgtgaagccg cgcgctttca tctgttcgcg aatttgttcg
     2761 gcgggaagat tcggcagaag cgcggagacc gtggcggacg cgtgcgcagc atcggacgcg
     2821 agcgtcgcgt atgcggacgt gcggcggatc gtcgcctcgc gcatggcctc atgacggggg
     2881 aagcgcgcgt cgagcgtttc gacgttgatg atgtcgtcga gctgcttcgc cagcgcgtag
     2941 aaatgcggaa tcgacggggt cgaaggaacg ctgccgtcat ccgcatgttt cttgtaattg
     3001 agaaaatcaa aataagttcc gcgatacggc ttcttcgccg ccctcgccaa cgcgtgggct
     3061 gaaacggcaa agagcgtaat gccgggcggc agtgcgatgc ccttctgcac gctggcaagg
     3121 cagacgtcga gtccccactc gtcaaatcgg actggaatac ccccgagcga agagacggcg
     3181 tcgaccagaa cgagcgtgtc gggggagtgt tcgcgcacga cgcgtgcgag cgccgcaacg
     3241 tcattcgtga cgccggtgga tgtctcgttg tgagtgatcg tcacggcgtc gtatcgtcct
     3301 cgccgcgacg taagatgatc ggccagagct tcgggctcga cggcgtttcc ccatcctgcg
     3361 ttgagttgat cgacttccag tccgaggctt tcgccgatgg cgcgccagcg ctcggaaaac
     3421 gcgccacagg tggttgcgag caggcggcgc ggtacgcagt tgatcagcgc cgcctcgaga
     3481 atcgccgtac cgctgcaggt ggcgacgagg gtgtcctgcg tcgtgccgaa gagtggcttc
     3541 aggttggcga tgatgcggac gaaaagctct ttgaactcag acgagcggtg accgatcatc
     3601 ggccgtgtca tctcgttcag aatcgccggg cgtacccacg ttgggcccgg cacgaagtag
     3661 gtggctcgtt ctgcggagtt gctcatgaga ggtgctggag tatgtcgcga aagtcggtta
     3721 cgtatttgtg cgcattttcc tttacgagag ggcggaccac aacgccgccg aagccgatga
     3781 aaagatcgac cgcatctttc gcctccagat cgctcacgcc gtccccgacg aacgcggcct
     3841 ttccgtgcga acgcgcacgc atgtcgcgta cgacgagctc cttaccaccc ggacgtgcaa
     3901 gaaacgagcg ccggtcgaaa tcctcgtaat cgccgcttgc gccgaagcgt aggcgaaccg
     3961 catgcacatt gcgctcagga atcccgagcg tttctgcgag cggaaggatt gcctgttcga
     4021 tgccaccggt gatcaggtgg acccgcgctc ctccatcttg aagtgcaacg atcgtctcct
     4081 gcacgccgtc gacgatcgag ttaacgtagg tcttccccag cttcgcaacg tcgtcacgcg
     4141 tcggcttgat gatgtcgagc cgcctcgcgt agacctgatc gagcggaatc aggccgttca
     4201 tcgcggcatc cgtgagcgcc gcgatttcgg gcgcgccgtt cgcgaggaca tcgattccct
     4261 cgatcgtaac gagcgtggag tcgacgtcga agaagatgag acggtaagtc atggttgacc
     4321 tgagactctg gcgtcgtcgc acacttcaat ctggcaaccc tccctcccca ccagatttac
     4381 caggtccgaa aacttctcca gcacaaagtc cggctgcgct gcggtcaact cggccgtagt
     4441 cgacgaccct gtcgcgatga cggccacgtc catcccgcta tttcgcgcgg cgcggacatc
     4501 gatcggcatg tcgccaatga aaagtgcgtc ggcgactgcg caccgggttg tctcgagtgc
     4561 gtgcaagaca tgtttcgcat cgggtttgct cgcccccgca agatcgggtc cgacgatggc
     4621 gctgaaatag ggcgcgagct ggaggaaggt caggatcttt ttcgagagcg acgttcgctt
     4681 gttcgtgcag accgccatga ctacgccaaa tccatggagt gtcttcaacg ttgcttcgac
     4741 gtcggccagg atcgtcgatt tctcacagca gatctcgtca tagcgtgttt cgaacgcgtc
     4801 gtgcgcactg cgtggaacgt cgtcggtatc gaaggagcgg cgtagcagca tgtccaagcc
     4861 gtcgccgacc gagccgcgga tgtgggcgat gctcagctcg tcgaggccgt gctcacggcg
     4921 cgcaaagttc accgcctctg tcagcgcggc gtaagaatcg accagagttc cgtccaggtc
     4981 gaacaggact gcgcggtaac gaagcggtgg caaggtcatt cggaagggcg gattctatcg
     5041 ccgtgcctat gagggacgtg gagaatggct ggatgaaaca tgccctggtt gcgctcctct
     5101 tcttcgcggt cccgctcttt gccgcggatg gcaagctgac cgtcgcgctc gagccgctcg
     5161 gcgattcggc tgagggggtt gtggcgcgcg tcagctttcg attcacggtt ccgaccgatg
     5221 cgccctccgg cgtgccgttg gagctcaccg gctcgatctc gcagaacgga caagtcgtca
     5281 aacgttttcg ttatccggtt ctgccgtcca caaccagtcc gattaccgcg atccagacgc
     5341 ttcagcccgg cgccgcggag atcgacgtgc gcctgatggt gccgctcgaa gagcagacgc
     5401 ccgtgattct ggcaaaggca acgcagacgt tcacggtcgc gaagacgggc actgcctacg
     5461 tagcccacga gggtgacgca gcggaagcga tactggccga gggggtggtg ccggagggga
     5521 ttggcgcggt gaagatcgtg ccgccgcggc gcgacgtggc gccgaacctt tttgtcgtcg
     5581 aagtcgaagt gatgccgcca gtgacacggg tcgagtggtg ggtcgaaggc aagaagatcc
     5641 tggcgcgcaa cgctcccccc taccgcgccg agctcgacct cggcgccctt ccgaaacgcg
     5701 tcgaagtgcg cgccatcggc tatgacgcgc agggacgcta tgtcgacgcc gatgccttcg
     5761 tcgtcaacga gcacgaaacg ccgctcgagg tgaagatcac gcgtacggtc acacccgacg
     5821 gcgtctccca cttcaagctc agtgtgcaga atccgaaagc gaccgtgatc aagtcggcgg
     5881 agttcttcgc cggacagaag aaactgcacg cgtggagcgg cccgccatac gcgctcgatg
     5941 tcccggccgc cgcgctgaaa ggcgtcgagt tcgttcgcgc atcggtgctc gacgaaacca
     6001 actacgaggc tagcgacctt ctctttctca acggcgcgcg tttctccgaa gagatcgagg
     6061 tgaacctcat cgagttgccg gttatggtgg tcgatcatgc gggcgttccc gtcacagatc
     6121 tcaagcagac ggatttcacg attctagaaa acggcaagcc tcagaaaatt acgaccttca
     6181 actacgcatc gaacttgccg atcgccgccg gcgttctgat cgatcactcg ggaagcatga
     6241 agcctcgcat ggcacaggcg aagacggcgg cgctggagtt cttcaagaag atcatgaagg
     6301 gcggcgaccg tgcctttgcc accggcttcg ccttcgacgc ggtctcgtcg gcgcctttcg
     6361 tgacggacgt tgggtcgctc gaacaacagg tgaatgcgat tccggacgcg agcggaggaa
     6421 cgtcgctcta cgacgccatc gtcaccggtc tctatcgttt ccgcacgctg caaggccgca
     6481 aggcgctgat catcctcact gatggcgaag acacgacctc gcgcacgagc tatgacgaca
     6541 tgctcacgta cgcccgctcc gcgcgcgtgc cgctctattt catcggtgtc gggcttggac
     6601 tcttcgatgc tagcggcatc tcgaagatga agacgctggc cgcggagacg ggcggcatcg
     6661 cctacttcat ccacgacatc aaacagctcg gcgaaacgta cacacaactg gagaaggatc
     6721 ttcgctcgca atacctgatc gcctacaaca cgcagagcac gggcaaggac aaggcgtacc
     6781 ggaaagtcga cgtcacggtg agccggccaa atacgaccgt acggacgatc cgcggattca
     6841 tcccgtgatg tttcgccgtc cggccgctgt tgtgctgatg ctggcgctcg cgtgttcgcc
     6901 ggcgccgcgt ccgcgcgacc gccacggcaa tccggtcgat gtcgacttgc gcggggcgcc
     6961 tgtcacgctt ctgcatttct gggcggcctg gtgcggcccg tgtcgcgagg agctccctcg
     7021 cttcatggca ttcgcagcta cacatcattt gagagtggtg gcggtctcgc acgaccgcga
     7081 cttcacgtcg gcagatcgct ttctgcgcca gcagggaatc ccgctcgaac gctactggac
     7141 gatcacggcc gcttcgccgc ccggtaccac gtccgcgtca ttccaacgac aatcgcgttc
     7201 aacgccgacg caagatcatc gaccgattcg atcagtcggt ggattggagc gatccggcgg
     7261 tcgcacgaag agtgctgagg tgattaagcc agagatcaga gacgagagat cagagacgcg
     7321 gcgctttcct gatctcttgt ctctaatctc caggcactgc ggcatcgcga gaagagataa
     7381 atcagagatc aagagataag aaaagccgcc ctcctgatct cttgtctctg ctttcctgat
     7441 ctctgctcct acgggtacat cctccgatac gacgacgcga tcatcaccac atgcttcaca
     7501 tacgtgcgcg tctcgcggaa cgggatcgat tcgatgaact cgtccatcga tttcgacggt
     7561 gcggcgcggc gccactgctg cacacgcccc tgaccggcgt tgtaggaggc gatggccagc
     7621 tcatcgttgc caaagagatc cacgagtcct ttgaagtgtg cggcgccgag gcggatgttg
     7681 gtctcgggat tgtcgagccg tggagcgatg tgaaagcggg ctgcgagctc ctttccggtc
     7741 gcgggcatga gctgcatcaa gcccattgcg ccaacgtttg atttcgcctt cggattgaaa
     7801 tagctctcct ggtgaatcag tcccatgacg gtgaagggat cgagatcatt cttcctcgaa
     7861 tagccacgga tccaatcgtc gtacttgagg gggtagtaca tcctcagaaa gtacgagggc
     7921 acggagtcct gctccacagt ggccagtgcg gggaaggcgc tacggagcgt cttcagcgtc
     7981 gattcggtgt cgcccgaggc gtggtagatc tcagccaaca gagcgtctgc gaattgctga
     8041 ttggcgcgat tgcggttcct gtcgatctcg agtcgtccgt cgcgaaagtc ggtgagcgcg
     8101 gtcagctcgt atcccaggcg gagagcttgg ggcatggtct gttcggcggt gtcgcgccaa
     8161 tccggacggt tcgacgtcag cggattgaca ttcgtttcag gccgtttcgc cccgcgcttt
     8221 tctgactcga tggcgtagat gtcgtcatac ggcgccgcgg ccagcttctg atacgtcgcg
     8281 gcggctgcgg ctttcctgcc gagccggtcg tcgcagcgcg cggaccagta ctccgcctgg
     8341 cgcttgacgt tcgggctgcg atatccatcg cggacaaacg ccatcagcgg gatcgcgcca
     8401 ttgagatcgc cgcgctcata cgccgcccac gctttgtccc agaaatgctg caggcctgcg
     8461 tcctgagagg gatcgacttt ctccaattga acaatgagct cctgttcata cgggtcctga
     8521 ttctttgcct cggcggccgc gatcagcctg ttcaggacgg tgatgcgaac ctcgtccgcg
     8581 agcggaagcg tgagcagatc cttcagccgt tccgtagcca gacgctcgta ctcatccctc
     8641 ttcgctttct tcgccaggtc gacgagttgg atgttcttct taacgttctt gaacttcggc
     8701 cttttcttgc cgctcttcag tgtgatggtg ccaacctgct gcttgacgac aacgctcttc
     8761 gtgactatcg ggttgatcga cgacgccaag gcaagatagt tctgggacgc ggtgaaaatc
     8821 gcgggaatgg cgaagcgata tgcgccggag gcgagcgggt cgagagcttt gttggactcg
     8881 tcgtaacgat gcaattgatt cagcgccagc ccgcgattga gctgaacggg agagcgcagg
     8941 tccggagggg gacccgcggt ttggagcgcg tccagctcgt cgaacgcgtg ttgcggatcg
     9001 ccgtcgcgca tgagggaaac cgcgcgctcg aggcgctcgc catccgtaag cttgatcggc
     9061 gcctcggcgg gaagtccggc gatggcgcgc gccat
//
LOCUS       DATLVW010000404        18101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_513530, whole genome shotgun sequence.
ACCESSION   DATLVW010000404 DATLVW010000000
VERSION     DATLVW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513530"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..464)
                     /locus_tag="VNN25_12100"
     CDS             complement(222..464)
                     /locus_tag="VNN25_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92314.1"
                     /translation="MPFYSSPENVLENPFSFRKRIVVALLSEVLAIAAIAAASFVLPI
                     VLPSLSAHGLIVIRWIGGFAAIVILCFGIALAVGEN"
     gene            complement(879..1610)
                     /locus_tag="VNN25_12105"
     CDS             complement(879..1610)
                     /locus_tag="VNN25_12105"
                     /inference="COORDINATES: protein motif:HMM:NF044290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92315.1"
                     /translation="MASDGAFEIIASLIVGVLTVVAALLTTRNSRRVSAEEEEIEKEV
                     QRQTTDGEGAPVVVEGHVPSSLTTPEYEQLIAKLTSDLLKKRAFAQFGNQAVAILTRS
                     YHKQALDQAKTQFWFGTVAATIGFAWILISGMFINNIAGTPKLIPGAIVGAVASLFLK
                     QSHETRQRATDFYDRLQSYERQRESVTLVTSIQDSQLQGAAQTLLALHMAGASTGDAV
                     TVLCRGSSERAVAAPGAVAKATAAG"
     gene            complement(1627..1923)
                     /locus_tag="VNN25_12110"
     CDS             complement(1627..1923)
                     /locus_tag="VNN25_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92316.1"
                     /translation="MVRLFNGTAIVESNNGLRIHEWGRESLAMSAATASTRVAVISLL
                     LWLLSFIVLAVSLLNEAASAQGVVRVVASTLYVLSFIAMIAAISAAIVFMMCQT"
     gene            complement(2003..3604)
                     /locus_tag="VNN25_12115"
     CDS             complement(2003..3604)
                     /locus_tag="VNN25_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92317.1"
                     /translation="MLVMLVLFLYLALREAKNSTSLFRYHLFGDDLKENRFTGSLIST
                     NASLSGAFVLILYYGFLYGPLAFPFVWSFWIITQLTSAWTINRTHAIMESHGGWTNNR
                     ATLHEFIGLVFDSPQSRRYAGLLSLISYLGLIAAEIVLATHLLEFVLPDNVRLPFSSL
                     ARGPFMVIAAVMLSILIYNVLSGFRGTVQTDYAQWLIISVMIVVVGIFVVAQWPFLWS
                     KYTTIFNAPSNGLVAAFFNPDHQGYFPYASFVLSNIVFWGLWWPGAMDQWQRCAAART
                     RRLSLNNIWGTVGFISLISFGALTFVFLAAGVWLRVKAPDALPSPALLREVVSGVQQW
                     ASSDLGRFGGLTLCALTFWGLVCAAMSTIDSYVMTASQALFVDVAHPQQGTTLRELSD
                     ADVEKKLLAQARAFTIFIPLLVLVFAYVFSLVSDVYALIYFAFAFMFALLPPLFVGLM
                     GWARPAARDACKRSLLAGGISTVLGYLVLLVGLERALRANDAGAIFKWYQGVYWWPTA
                     VTLIGSIVLWFSWPRGKVTEVASND"
     gene            complement(3954..4664)
                     /locus_tag="VNN25_12120"
     CDS             complement(3954..4664)
                     /locus_tag="VNN25_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXH92318.1"
                     /translation="MSWTIGHGETHVWLAATNLPPELLARAATLLDDEESRRVASFRF
                     ESDRRLSLIARAARRVLLGRYTARDPRALRFVEGPQGKPSLTTGELEFNVSHSGSHVA
                     VAISVGGAVGVDIEAVRATSDLVHLATRFFSPHEAEIVRTAPDDERASRFFAYWTAKE
                     SVIKAVGGGLSLDLRSFQTNPRLGHATPVQNLSGDSRLDGWNVFALPSGIEAVHLALA
                     SRGTEMPRISELDSSLLA"
     gene            4710..5933
                     /locus_tag="VNN25_12125"
     CDS             4710..5933
                     /locus_tag="VNN25_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXH92319.1"
                     /translation="MNTSIACSGCKYIAPPFDPRPFRCPRAGDGGEHIFVRRLARVDA
                     ASFFDPEPNPFIRYRQLTHAWNTAMALGITDSEFVALVRELDERVAAVAGVGFVETPL
                     CRGSITIKDETANVAGSHKARHLMGLMIWLRIAEQIDPSLADARLAIASCGNAALGAA
                     VIARAAERPLDVFIPLDAAPSIVTRLETLGAHITRCARNGGEAGDPAYLRFREAVAAG
                     ALPFTCQGSENGLVIEGGQTLGWEIASQIAAAGMTVDRLFIQTGGGALASACIAGLEE
                     ARAAGVIPRLPRIHAVQTSAVAPLQRAWQLVETHGLDSATANRAQFMWPWETPGRSIA
                     TGILDDETYDWLAVVRGMQSSGGFPVVVSEEELMAAKELAGDGVSATGVAGLAGLLHL
                     RVSGEVQMVLFTGAA"
     gene            complement(5917..6468)
                     /locus_tag="VNN25_12130"
     CDS             complement(5917..6468)
                     /locus_tag="VNN25_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH92320.1"
                     /translation="MAYVVAVSGISGAGKTSVIRRASELLESAVGLYFDDYAAASTYP
                     PDLKEWVERGANVDEWQTPQLAADLRALREGPNVVVLVEEPFGKLRREMAGLIDLAIH
                     LEVPADVLLARRLMRRIEEQRDSKTLIARLEDDLRHHLAAGRKLDALGSNAAAKAADV
                     VVDATMSIDEIAERVVAEIRRRL"
     gene            complement(6479..7225)
                     /locus_tag="VNN25_12135"
     CDS             complement(6479..7225)
                     /locus_tag="VNN25_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH92321.1"
                     /translation="MKRTDSERWLAYTNVAADHRTNVFCFPFAGGGASFYRAWSQFTP
                     PRIALCPLQPPGREERFVETPFDSMASLVLGAADALAPHLSRPYALFGHSMGALACFE
                     IVHLLAARGAPLPVHLFVSGAPAPQLASLIPPVYDLPQEELLQEVQRYGGLPDEVLRS
                     PELLDLLIPRLRADFAVTGTYRYASRPPLHLPITAFTGQDDQIVTPSMVDGWREQTSS
                     AFRLELFDGGHFFLARHAREIVLKLAHALA"
     gene            7255..8595
                     /locus_tag="VNN25_12140"
     CDS             7255..8595
                     /locus_tag="VNN25_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92322.1"
                     /translation="MTVRNGSASLSRRLAFGSESYYFFDKMFCQQPLARISRIGDMAT
                     ARIPDHMARVAYDLVESIRTRRWTPDPGNAELAMLEATDLLPQNRRYLSYWHDPAINT
                     TAGTIVGVDLIRHNGKYHVIEINRGPSIYPRRREMYAEPFDPMFWRIFETAPAEGYRR
                     IVPIAFRWHAVYIEEFERASRALGIPIIPYNCPLQFPECPRRMFALPDPVEPETLYVI
                     HSGLMSPTVRYLDDKWYVWQWLERAIREEMPGTLVALPTTHDDLVFPMDDHGPRWPNL
                     VVKLASSARSCHVIAARFADIDEAREALGVSGIGQIPAKLRPAFLKGLLFRGSERVLY
                     QSFVPPELDEREHARMLRLHIWVSPQRTTYLSAHARISHKPIPKVAPRGIIRQDDAYV
                     FNNADYALLSPEIEEEIRSVAADLGGAMQRALVRKFETGPPQANSSAGSTTAIR"
     gene            complement(8553..13196)
                     /locus_tag="VNN25_12145"
     CDS             complement(8553..13196)
                     /locus_tag="VNN25_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HXH92323.1"
                     /translation="MSPSDDHGALLKKALREIKSLRSGLAEAKRANREPIAIVGMGCR
                     FPGRANDPAAFWELLRSGTDAITEVPRSRWDVDALYDADPDAAGKMYTRHGGFLDGMD
                     GDIDQFDAEFFGISPLDAMNMDPQQRLLLEVAWEVFENAGIVPDKAPRTGVYVGSFMD
                     DYLQSNFFAADRNDIDAYNTLGLLRGLAAGRLAYVLNLQGPAMQLDTACSSSLLTAHL
                     ACQALRNRECDLALAGGVNLILAPEVTIGLCRMRAMAADGRCKSFDASADGYVRGEGC
                     GLVVLKRLSEAVADGNRIYAVIRGSAVNHDGRSNGLTAPNGTAQKTLIREALDAAGVS
                     PKQIQYLEAHGTGTSLGDPIEAMAVGEVLCRERTEPLLLGSVKSNVGHLESAAGVAAL
                     MKTALALHHGAIPPSLHFRDPNPHIPWDRLAVTVPTALTAWPKGESRFAGVSSFGMSG
                     TNVHMILEQAPAAAAPLDREAPHVLTLSAPNEAALEALANNYVPFLEGNASSLRDICD
                     SSNRGRKHFDHRLAIAAESAESLVAGLRRPHASSKRRKRPRIAFLFPGQGVPLAGDTA
                     GSIQAKLFTYEVELARQWIACGIRPDAVIGHSVGEYAAACIAGVFSFDDGLKLIAERE
                     RLMNALPERGAMAAIFADEDRARDAIGALDVSIAALNGSHVVISGRAADVRAVAERFE
                     ARMLDVANAFHSSLMDPMLDEFEAAVGRATLRRPSLQFISAVTGAAADAELTNAAYWR
                     QHVRHPVRFADCIRNVSADVFIEIGPKPTLISMTRHVLDRDDVVMLAGLAAESLATLY
                     ERGLDIDWSAVSPKAATVTLPSYPFQRRSFWIQKRNARVQQGARKIPFSNDTHIELTL
                     SPERPRSLKDHRIGEAIVVAAGAYLSMALESLEATTLRDVTFLQPLTIDAETSLHLVA
                     TDSTFRFGTERGGEWITHCTGSYERGAASSEVTLPDFEGEPVDADIDAGLDAGLNLGS
                     SFRWTRSISRRGEEVFCRMEAPPSIEGAAFHPGLIDSCLRTRALGLDGIHVPFHIDTL
                     RVFRKPKGTLLCHSTSGAIRLFDDESLVMEILGFQVRPLARSKELPLYRVEWQRAEGG
                     AVEGVRHVDVFAFDNILDLRDTPNVRLITHGAQAVREGDRVDPTNAWVWGLAKVVAIE
                     QPQLRWSCVDLDPDLSVGEQLSLLQNVPHENQVAFRGGEAYVPRLVPATTGATAGATA
                     RVRDDCAYVITGASGALGGHVARWLVRQGARHLILVSRSTPNLDDLGDDVRIVNADVA
                     DADSLAPVFDSAVPIKGIIHAAGVLDDALLYDMSRERFARVFAPKVAGTWNLHQRSLG
                     LDLDFFVCFSSAAALIGSAGQANYAAANAYMDALMDHRRALGMPGLSINWSAWAEGGM
                     AGNSAERMTSIGIEMIDPSAGLETLGHLLQYDGAQVGVIPADWSKLLPSMFDAPPPFF
                     SRLIAPKSKTTPAGILPLLENTPRDERRQRLEDHLRQIVVAVAGRNVFDGMSEDANFF
                     ELGMDSLMALDLRNRLQTELDRALPSTVALEYPGVRELADYLIAVVLPAELFA"
     gene            complement(13200..16769)
                     /locus_tag="VNN25_12150"
     CDS             complement(13200..16769)
                     /locus_tag="VNN25_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HXH92324.1"
                     /translation="MVDDTVILRGKDLTIDEVARVARGGAKVSITTDARVEQKVRGSQ
                     EYVTRLIAAGERLYGVTTGFGGKSNTAITAGDACDLQEALLWFLKSGAGGRLPAGDVR
                     ASMLLRANSLLCGVSGIRMELIRRFETFLNNGLTPHVRELGSIGASGDLVPLAAIAGA
                     IVGLDNSFKVDVRGKEMGAIDALRTLGLEPERMAPKEGLALVNGTSVMTAIAMNAVYD
                     SRVVIALAMGAHALMFQAMRGNAHALHPFIHHHKPHPGQVWVARNLFKLLKGSKWTYG
                     ETRGGFEHSAGSLAQDRYSLRCIPQYLGPVVDGLAVIARQMETEANSATDNPLFDGEG
                     ESYYEGGNFLGQYVGVGMDQLRQYLALTAKHLDTQIAMLVAPEFNNGLPSSLVGNEAR
                     SVNNGLKGLQLTGNSIVPELLHLASPMADRYLTHAEQFNQNVNSLGFGSANLARRAVS
                     FSQQYLAVALMFGVQAADLRAHREEGHYDGRAGLSPATVPLYEAVCSICRVKPGRERA
                     FVHDDRDQSLDIYIEELAADIAGDGAIPNALQDTLQALKEPGITLVSRQEEQVEMNVA
                     QNIIRGRKLFPRKVALTFENRHYTYADLDEWSNRVAEGLAASGVGRGDRVALFLPNIP
                     EFAAAYLAIQKLGAVAVSLNSTLRTEETRFILNDSGAVALFTTEVLRGHVDRAASPEL
                     KLVVIVEGAASGTDTSLSDLTANTTGEFTPAIMDRDDPSAILYTSGTTGEPKGACLSH
                     GNVISNMHAFNYNCGMRVDDKLLLFLPLFHCFGQNAILNSGFNACATVVLHRTFHPAT
                     IIRSLADDQVTMFFGVPTTFIPLYNLASAAELESVRYYFSAAATLPREVARKWEEKYR
                     RPINEGYGLTETSPFASYNHRLWYKLGSIGAPIENVEMRVVSPDTGAEVATGDPGEIV
                     VRGPNVMLGYWNRPEATAQAIRDGWFHTGDIGRVDEDGYFYIVDRLKDMVNVGGLKVY
                     PVEVENTIYLHPAVEEVAVYGIPDQVMSERVCANIVCKKEHSATEAEIMAFCRQQLAD
                     YKIPSAIRFVEALPKSPTGKILKRVLRDEGRTAFPVAATARAVGALEIDMWIREWITD
                     NLEVDGSTIAADRSFFEFGMTSLTGLKLSAQLGEWIGRPMEDVLVWNHGNIDALVKYT
                     AESAKLEVLAKIGDIDDIDNLADDEVARLLAAELAGGDTSEPD"
     gene            complement(16776..17798)
                     /locus_tag="VNN25_12155"
     CDS             complement(16776..17798)
                     /locus_tag="VNN25_12155"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXH92325.1"
                     /translation="MTITAWHEPKTEFWAKNEPMRESPNWHGVSPEVIARRGRPWNQD
                     PNDVPWLDRDDAEERIQSRLAEGLISSEEAELLRQWVVEGYFILRGAIDQSDFGTIDQ
                     FNRFLDDLWTTDQVIPGLHIMSLHIKGRPPGPIRHAELLQWPLEYRLELRDQQLWRVH
                     YLHCHSEAMLALTMSRHLLRMATLILEEPAVLASSIGFKWGSEVGVHQDLAAMHIHPK
                     NRLVGVWLAGEDVDPLAGPLGVFPGSHRVPIWPGWNNYPQTNLRTCHLDTRSKEEQYL
                     KDAVAGGQRVSLPVKKGDAILTHGLMIHGGDKIKNRALTRFSAVLHYTVPGGDKTDEI
                     TGPFNW"
BASE COUNT         3381 a         5774 c         5703 g         3243 t
ORIGIN      
        1 cgactggcct actctcagtg aggaagccgg ccgcgagctc gctcgagccg tgcgcgaggc
       61 gttcgggagg gatgacattg cagtttgacc ggctcgcctt cgacgcgaac gccgccatcg
      121 attcaaatgc gccccgcacg cccctttcca ctcccatgag tagaaacgcg tcgccagaaa
      181 attgaaaaag atggcactaa aatagctgcg cgtgaagtcg cctagttctc tccgactgcc
      241 agggcaatcc caaaacacag aataacgatc gcggcaaatc cgccgatcca tcgaatcacg
      301 atgaggccgt gtgcactaag cgatggcaga acgatgggaa ggacgaaact tgctgccgcg
      361 atcgctgcaa tggccagtac ctccgacagc aacgcgacca caattcgctt ccggaatgag
      421 aacggattct ctagtacatt ctcgggtgac gagtaaaagg gcatgagagg cttccgtatt
      481 tttgagttgt tgtttgatga cgatgccgct cactaattac cgcacagatc cggcccttct
      541 gccaacccaa ggcagcagca cacgagcggt gcgtgacaaa acaacccttt cagacgggga
      601 cacttgatgg atcataccag tttcgaacca gaaactcgac tctcccagat cacgacagcg
      661 cagctgattc aacggcctcc actctacctc aaacgaagta ccaggtgcca gctaccgttc
      721 tctcggctgc aaccggacgc cgttttcctc gaggcaggct ccaacgatag aacgtgcgcc
      781 cggtggcctg atggcccgtc cgctcacggt cgcgctctct gctcatgaca tccggcaacg
      841 ctcacgggcc agctcagcac ggcactgttc tgtccgcatc atccagccgc ggttgccttc
      901 gccacggcac ccggtgccgc gactgctcgc tcggacgacc cacggcagag aacggtgact
      961 gcgtccccag ttgaggcccc tgccatgtga agagccagca acgtctgtgc agcgccctgc
     1021 aattgagaat cctgaatcga cgttacaagc gtcacagatt ctcgttgtct ctcatagctt
     1081 tgcagtcgat catagaaatc cgttgccctc tgtcgcgtct cgtgagattg cttcaaaaac
     1141 agacttgcca cggcacccac gattgcgccc ggaattagct tcggcgtccc cgcgatgtta
     1201 ttgatgaaca tcccgcttat gagaatccat gcgaagccga tcgtggcggc aacggtgcca
     1261 aaccaaaact gcgtcttcgc ttgatcaagt gcctgcttgt ggtagcttct ggtcaagatc
     1321 gccacggctt ggttgccaaa ttgtgcaaag gcacgcttct tcagtaagtc agacgttagc
     1381 tttgcgatca gttgctcgta ttccggcgtg gtcaaactac ttggtacatg gccttctact
     1441 actacgggcg cgccctcgcc atcagtcgtc tgtcgttgaa cctctttctc gatctcctct
     1501 tcctcggcag agactcttct gctattcctg gtcgtcaaca acgccgcgac gaccgtcaaa
     1561 acgcccacta tgaggctagc aatgatttca aacgcgccat ccgatgccat cactccattc
     1621 ctcgtttcag gtctggcaca tcataaatac aatggcggcg ctaatcgccg cgatcatcgc
     1681 aataaagctc aacacataca gcgtgctggc cacgacccgc actactcctt gagcgctcgc
     1741 tgcctcgttc agcagtgata ccgcaagcac aataaaactt agaagccaca gaagcaggct
     1801 tatcacagcc acgcgcgtag aggcagtcgc tgcgctcatc gcaagcgact ccctacccca
     1861 ttcgtggatc ctgagaccgt tattcgactc gacaatcgct gttccattga ataaacggac
     1921 catgaaaccc acggcgacgc ccaggccgaa tgtgcatatc gcgaatagcg ctagaaacct
     1981 agattccgac ctacgcaatg actcaatcat tgctggcgac ctccgtaacc ttccctctgg
     2041 gccaagagaa ccacagtacg atcgatccga ttaatgtaac ggccgtcggc caccagtaaa
     2101 caccctgata ccatttaaaa attgctccgg catcgttcgc tctaagtgcc cgctctagtc
     2161 ccacgagcaa gactagatag cccaggaccg tgctgatccc tccagccagc agactgcgtt
     2221 tgcaggcgtc acgagcggct ggccgtgccc atcccatcaa accgacgaat aacggtggaa
     2281 gcaacgcaaa catgaatgcg aacgcaaagt agatgagcgc gtacacatct gacactaatg
     2341 aaaacacgta cgcaaacacc agaaccagga gcggaatgaa gatcgtgaag gcacgagcct
     2401 gtgctagcag tttcttttca acatctgcgt cgctgagctc tcgcagggtg gtgccttgct
     2461 gcggatgtgc gacatcaaca aacaacgctt ggctggccgt cataacatag ctatctattg
     2521 tcgacatcgc ggcgcagacc agaccccaaa atgttagagc gcacagtgtc aatccaccaa
     2581 atcgaccaag atcggatgag gcccactgct gcactcctga gacgacttcc ctgagaaggg
     2641 ctggacttgg caatgcatct ggtgctttaa ctctcagcca aactcccgct gccagaaaga
     2701 caaatgtcag ggccccaaag gatatgagcg atatgaagcc aacggtaccc caaatattat
     2761 tcagtgacag gcgtcgtgtg cgtgcggcag cacaccgctg ccactgatcc atagcgccgg
     2821 gccaccacag gccccaaaat acaatattcg aaagaacgaa cgacgcatac ggaaagtatc
     2881 cttggtggtc cgggttgaag aatgccgcca ccaaaccgtt tgatggcgca ttgaagatcg
     2941 ttgtatattt tgaccataga aagggccact gagcgactac gaaaataccg accaccacaa
     3001 tcatgacgga aatgatcagc cactgtgcgt aatccgtctg cactgtcccg cgaaaaccag
     3061 acaagacgtt ataaattaag atcgataaca tcacggctgc tatgaccatg aacgggccgc
     3121 gagcgagaga actgaagggc aaccgcacat tatctggtag cacaaactcc agcaggtgag
     3181 ttgcgagcac gatctcggcg gcaataaggc ctaggtacga tatcaggctg agcagtcctg
     3241 catagcgccg cgattgaggt gaatcaaata ccagcccgat gaactcgtgg agcgtagccc
     3301 tattgtttgt ccaaccgccg tgagactcca tgatcgcgtg tgtgcggttg atcgtccatg
     3361 cggacgtaag ctgcgtaatt atccagaatg accaaacgaa tggaaaggct agcgggccat
     3421 ataggaagcc atagtagaga attagaacga acgcgcccga gaggctcgcg ttggtcgaga
     3481 tcagagatcc cgtgaaccga ttctccttga ggtcgtcacc gaagaggtga tatcgaaaga
     3541 gggaagttga gttcttcgcc tcacggaggg cgagatagag aaagagaacg agcatgacaa
     3601 gcattccggc accgagtagc ggctgaagcg ctgaatctga catccgatag ctcctttcgg
     3661 cctttgttct agcgttgtcg tcatccaaga tttgcgcgag gagtgcgatg tgctgctcgc
     3721 aaggcgagcc tgatccgccg gttatttgct gaactgagtt ccatggtggt ggacgcccta
     3781 ttaagaggag tgtcatttga gggcatttcc ctacccacca agactggaag gcactcgcga
     3841 tctccggcgt tgatcagcgt gcaaccccgt ctcacctccg aaccttctcg actaatgtca
     3901 gaagccttgc gtggaccagc gagcagccac agagttcccg cttcgcaaac acctcacgcc
     3961 agcagcgacg aatcgagctc acttatgcgc ggcatctccg tcccccgcga agcgagtgca
     4021 aggtggacag cctcgatgcc gctcggcagc gcgaacacgt tccagccgtc gaggcgcgaa
     4081 tcgccgctga gattctgcac cggcgtggcg tggccgaggc ggggattggt ctggaacgag
     4141 cgcagatcga gggaaaggcc gccgccgacc gctttgatca ccgactcctt cgcggtccag
     4201 taggcgaaaa agcgtgacgc gcgctcgtcg tcgggtgcgg tgcggacgat ttccgcctcg
     4261 tgcggggaga aaaaacgcgt ggcgaggtgg accaagtcgc tggttgcgcg caccgcttcg
     4321 atgtccacgc ccaccgcacc acctacggaa atggcgacgg cgacgtggct gccggagtgg
     4381 ctgacgttga actccagttc gcccgtcgtc agcgacggtt tgccttgcgg accttcgacg
     4441 aagcgaagtg cgcgcggatc gcgggctgtg taacggccga ggaggacgcg gcgcgcggcg
     4501 cgggcgatca gggagaggcg gcggtccgac tcgaagcgga acgaggccac gcgccggctc
     4561 tcctcgtcat cgagcagagt ggccgcgcgc gcgagcaact ccgggggaag gttcgtggcc
     4621 gccagccaga catgcgtctc gccgtggccg atcgtccagg acacctggcg atgataaagg
     4681 atcgagtcag cgacgtatcc ttgtcgcgaa tgaacacctc gatcgcctgc agcggctgca
     4741 aatacatcgc gccgccgttc gatccgcggc cgtttcgttg tccccgtgcg ggagacggcg
     4801 gcgagcacat cttcgttcgg cggctggcgc gggtcgatgc ggcctcgttc ttcgaccccg
     4861 agccgaatcc gttcatccgt tatcggcaat tgacgcatgc gtggaacacg gcgatggccc
     4921 tcgggatcac cgactcggaa ttcgtggcgc tcgtgcgcga gctcgacgag cgcgtcgcgg
     4981 cagtcgccgg cgtagggttc gtcgaaacgc cgctctgccg cggctcgatc accatcaaag
     5041 acgaaaccgc gaacgtagct ggctcgcaca aggcgcgtca cctgatggga ctgatgatct
     5101 ggctacgcat cgccgagcag atcgatccgt cgctcgccga tgcgcggctg gccattgcca
     5161 gttgtggcaa cgcagccctc ggcgctgcag tcatcgcgcg cgccgcggaa cgtcctctcg
     5221 acgtcttcat ccccctcgac gccgcaccat cgatcgtcac ccgcctcgag actctcggcg
     5281 cacacatcac ccgctgcgcg cgcaacggcg gcgaggccgg cgatcccgca tacctcaggt
     5341 ttcgcgaggc ggtggcggca ggagcgctgc cgttcacctg tcaggggagt gagaacgggt
     5401 tggtcatcga aggcggccag accctcggat gggagatcgc gtcgcagatc gctgccgccg
     5461 gcatgaccgt cgaccggcta ttcattcaga ccggcggcgg tgcgcttgcc agcgcctgca
     5521 tcgccgggct ggaagaggcg cgcgcggccg gagtcattcc gcggctgcca cgcattcacg
     5581 ccgtgcagac cagcgccgtc gctccgctgc agcgcgcgtg gcaactcgtc gaaactcacg
     5641 gcctcgattc cgcgaccgcg aatcgcgcgc agttcatgtg gccgtgggaa acgccgggcc
     5701 gcagcatcgc cacaggcatt ctcgacgacg agacatacga ctggctcgcg gtcgtgcgcg
     5761 gcatgcagag cagcggcggc tttccggtcg tggtgtccga agaagagttg atggcagcga
     5821 aggagcttgc gggcgatggc gtcagcgcga ccggcgtggc agggctggcg ggcctgcttc
     5881 acctgcgcgt gtcgggcgaa gtgcaaatgg tgctgtttac aggcgccgcc tgatctcggc
     5941 gacgacgcgt tccgcgatct cgtcgatgct catggtcgca tcgacgacga cgtcagcggc
     6001 tttcgcggct gcgttcgagc cgagggcgtc gagcttgcgg ccggccgcga ggtgatgccg
     6061 caagtcgtcc tccagccgcg caatcagcgt tttcgagtcg cgctgttctt cgatccgtcg
     6121 catgaggcgc cgcgcgagca ggacgtcggc cgggacctcg aggtgaatcg ccagatcgat
     6181 gaggcctgcc atctcgcggc gcagcttccc gaacggctct tcgaccagca ccaccacgtt
     6241 gggaccttcc cgcaacgccc gcaggtcggc ggccagttgc ggagtctgcc actcgtcgac
     6301 gttcgcgccg cgctcgaccc actccttcag atcgggcgga tacgtcgacg cggccgcgta
     6361 gtcatcgaag tacaggccca ccgccgactc gaggagctcg gaggcgcgcc ggatgacgct
     6421 cgttttcccc gcgccggaga tcccgctcac tgcgacgaca taggccatcg gttgcacctt
     6481 atgccagcgc gtgggcaagc ttcaacacga tttcgcgcgc gtgccgggcc agaaagaaat
     6541 gtccgccgtc gaaaagttcg agccgaaacg ccgacgacgt ctgctcgcgc catccgtcga
     6601 ccatcgacgg cgtcacgatc tgatcgtcct gtcccgtgaa cgcggtgatc ggcaggtgaa
     6661 gcggcggccg gctggcgtat ctgtaggtgc cggtcacagc gaaatcggcg cgcagccgcg
     6721 ggatcagcag gtcgagcaac tcggggctgc gcagcacctc gtccggcagg ccgccgtacc
     6781 gctgcacttc ctgtagcaac tcctcctggg gaaggtcata gaccggcgga atgagcgatg
     6841 cgagctgtgg cgccggcgct cctgagacga agagatgtac cggcagcggg gcgccgcgtg
     6901 ccgccaggag atggacgatc tcgaagcagg ccagcgcgcc catgctgtgg ccgaagagcg
     6961 cataaggcct cgacagatgc ggcgccagcg catcggctgc cccgaggacg agcgaggcca
     7021 tcgagtcgaa cggcgtctca acaaagcgct cctcccgccc cggtggctgc agggggcaga
     7081 gggcgatccg cggcggcgtg aactggctcc aggctcgata gaacgacgcg cctccgccgg
     7141 caaacgggaa gcagaagacg ttcgttcggt gatccgcggc gacgttcgtg taggccagcc
     7201 agcgctcgga gtcggttctt ttcatgcgga aagcgcgcta ttgtcaccga ttcaatgacg
     7261 gtgcgaaatg ggtccgcttc gctgagccgc cgcctggcct ttggaagcga gtcctattac
     7321 ttcttcgaca agatgttctg ccagcagccg ctggcccgca tcagccgcat cggcgacatg
     7381 gccacggcgc gcattccgga ccatatggcg agagtcgcgt acgatctcgt cgaaagcatc
     7441 aggacccgca gatggacgcc ggacccaggc aacgccgagc tggcgatgct ggaagcgacc
     7501 gatctgttgc cgcagaaccg gcgctacctg tcgtactggc acgatccggc gatcaacacg
     7561 acggccggga cgatcgtcgg cgtcgatctg atccggcaca acggcaagta ccacgtcatc
     7621 gagatcaacc gcgggccttc gatctatccg cgtcgccggg agatgtacgc ggagccgttc
     7681 gatccgatgt tctggcgcat cttcgagact gcgccggcgg aaggttaccg gcggatcgtg
     7741 ccgatcgcct ttcgctggca tgcggtctac atcgaagagt tcgagcgggc atcgcgcgcg
     7801 ctcggcatcc cgatcattcc gtacaactgc ccgttgcagt tccccgagtg tccgcggcgg
     7861 atgttcgcac ttccggatcc ggtggagcct gagacgctct acgtcatcca ttcgggactg
     7921 atgtcgccaa cggtgcgcta tctcgacgac aagtggtacg tgtggcagtg gctggagcgt
     7981 gcgattcgcg aggagatgcc cggcacgctg gtcgcgctgc ccacgacgca cgacgatctc
     8041 gtttttccga tggacgatca cggaccgcga tggccgaacc tggtggtgaa gctcgcatcg
     8101 agcgcgcgaa gttgccacgt gatcgctgcc cgcttcgcgg acatcgacga ggcccgcgaa
     8161 gccctcggcg tctcaggcat cgggcagatc cccgccaagc tccgtcccgc ctttctcaag
     8221 ggactgctct ttcgcggcag cgagcgcgtg ctctatcagt ctttcgttcc acccgagttg
     8281 gacgagcgcg agcatgcccg gatgctgcgg ctgcacatct gggtgtcgcc gcagcggacg
     8341 acgtatctct ccgcgcacgc aaggatctct cacaagccga ttccgaaggt cgcgccgcgc
     8401 ggcatcatcc ggcaggacga cgcctacgtc ttcaacaacg ccgactacgc gttgctgtcg
     8461 ccggagatcg aagaagagat tcgctcggtc gccgccgacc tcggaggcgc catgcagcgc
     8521 gcgctcgtgc ggaagttcga gactggccca cctcaggcga acagttcggc cggaagcacg
     8581 accgcgatca ggtagtcggc gagctcgcgc actcctgggt actcgagcgc caccgtcgac
     8641 ggcaacgcgc gatcgagctc cgtttgcagg cggttgcgga gatcgagcgc catcagcgag
     8701 tccatcccca gctcgaaaaa gtttgcgtct tcggacatcc cgtcgaacac gttgcggcct
     8761 gcgacagcga cgacgatctg gcggagatgg tcttccagcc gctgccgccg ttcgtccctc
     8821 ggtgtgttct ccagcagtgg caggatgccc gcgggcgttg tcttgctctt cggtgcgatg
     8881 agtcgggaga aaaaaggcgg cggcgcatcg aacatcgacg ggagcagctt cgaccagtcg
     8941 gccggaatca cgcccacctg cgcgccgtcg tactgcaaaa ggtgtccgag ggtctcgagg
     9001 ccggcggaag gatcgatcat ctcgatcccg atcgacgtca tcctctccgc gctgttcccg
     9061 gccatccccc cctcggccca ggcgctccaa ttgatcgaga ggcccggcat tccgagtgcg
     9121 cggcggtggt ccatgagcgc atccatgtac gcgttggccg cggcgtaatt cgcttgtccg
     9181 gcagaaccga tgagcgcagc ggcggacgag aagcagacga agaaatcgag gtcgagtccg
     9241 agactgcgct ggtggagatt ccacgtgccg gcgaccttcg gcgcgaacac gcgcgcaaag
     9301 cgctcgcggc tcatgtcgta caggagggcg tcatcgagca cgcccgcggc gtggatgatt
     9361 cccttgatcg gcaccgccga gtcgaagacc ggcgcgagtg agtctgcgtc ggcgacgtcg
     9421 gcattgacga tgcggacgtc gtcgccgagg tcgtccagat tcggcgtcga gcggctgacc
     9481 agaatcagat gccgcgcacc ttgccggacc agccagcggg cgacgtgccc gccgagggcg
     9541 cctgaggcgc cggtgatgac gtaggcgcag tcgtcgcgga cgcgagccgt cgcgccggcc
     9601 gtggcaccgg tcgtcgccgg caccagccgc ggcacgtacg cctcgccgcc gcggaaggcg
     9661 acctgattct cgtgcggcac gttctgcagc aggctcagtt gttcgccaac ggagaggtcc
     9721 gggtcgagat cgacgcagct ccagcgcaac tgcggctgct cgatggcgac gaccttcgcc
     9781 agaccccaga cccacgcgtt cgttggatcg actcggtcgc cctcgcgcac cgcctgcgcg
     9841 ccgtgcgtga tgagacggac gttcggcgtg tcgcgcaagt cgagaatgtt gtcgaacgcg
     9901 aacacgtcga catgacgcac gccctctacg gctccgccct cggcgcgttg ccactccacg
     9961 cgatagagcg gcagttcctt cgagcgggcg agcggccgca cctggaagcc gaggatctcc
    10021 atcaccagcg attcgtcatc gaaaaggcgg atggcgccgc tcgtcgaatg gcacaaaagg
    10081 gttcctttcg gcttgcggaa cacgcgcagc gtgtcgatgt ggaagggaac gtggatgccg
    10141 tcgagtccga gagcgcgcgt gcgcaggcac gagtcgatca ggcccggatg aaacgccgcg
    10201 ccttcgatgg acggcggcgc ttccatgcgg cagaagactt cctcgccgcg gcggctgatg
    10261 ctgcgcgtcc atcggaacga cgagcccaga ttaaggccgg catccaggcc ggcgtcgata
    10321 tccgcgtcga ccggctcgcc ctcgaagtca ggaagggtga cctcggatga cgccgcgccg
    10381 cgctcgtacg agccggtgca gtgcgtgatc cattcgccgc cgcgctcggt gccgaaacgg
    10441 aacgtactgt ccgtagcgac gagatggagc gatgtttcgg catcgatcgt gagcggctgc
    10501 aggaatgtga cgtcgcgcag agtcgtggcc tcgaggctct ccagcgccat cgagaggtac
    10561 gcccccgcgg cgacgacgat cgcttcgccg atgcggtgat ctttcagtga tcgtggccgc
    10621 tccgggctca gcgtgagctc gatgtgcgtg tcgttcgaga aggggatttt tcgcgctccc
    10681 tgctggacgc gcgcgttgcg tttctgaatc cagaacgacc ggcgctggaa cggatacgac
    10741 ggcagcgtga ccgtcgccgc cttcggactg acggcgctcc agtcaatgtc gagaccgcgc
    10801 tcatagagcg tcgccagcga ctcggcagcg aggccggcca gcatgaccac gtcgtcgcgg
    10861 tcgaggacgt ggcgcgtcat ggaaatgagc gtcggcttcg ggccgatctc gatgaacacg
    10921 tccgcgctca cattgcggat gcagtcggcg aagcggacgg gatgccgaac gtgctgtcgc
    10981 cagtacgcgg cattcgtcag ttccgcgtcg gcagcggcgc cagtgacggc ggagatgaac
    11041 tgcagcgacg gcctgcgcag cgttgcacga ccaacggcgg cttcgaactc gtcgagcatc
    11101 ggatccatca ggctcgagtg aaacgcattc gcgacatcga gcatccgggc ctcgaaccgc
    11161 tccgcaaccg cgcgtacgtc ggcggcccgg ccggagatca ccacgtgcga gccgttgagg
    11221 gccgcgatcg acacgtccag cgcgccgatg gcatcgcggg cgcggtcttc atcggcgaag
    11281 atcgccgcca tcgccccacg ctcgggcagc gcattcatga gccgttcgcg ctcggcgatc
    11341 agtttcagac cgtcgtcgaa actgaatacg ccggcgatgc acgccgcggc gtattcgccg
    11401 acgctgtgcc cgatcaccgc gtcgggccgg atgccgcagg cgatccactg cctggccagc
    11461 tcgacctcgt acgtgaagag cttcgcctgg atcgagccgg ccgtgtcgcc ggcaagggga
    11521 acgccctggc cgggaaagag gaatgcgatt cgcggacgtt ttcgccgctt gctcgacgcg
    11581 tgcgggcgtc gcagccctgc cacgagcgac tcggcggatt cggccgcgat cgccagccga
    11641 tgatcgaagt gcttgcggcc gcgattcgag gagtcgcaga tgtcgcgcaa gctcgaagcg
    11701 tttccctcga ggaacgggac gtagttgttc gcgagcgctt cgagcgcggc ttcattcggc
    11761 gccgaaagcg tgaggacgtg aggcgcctcg cgatccaacg gagccgcggc cgccggcgcc
    11821 tgctccagaa tcatgtgtac gttcgtgccg ctcattccga acgagctgac accggcaaat
    11881 ctgctctccc ccttcggcca cgcggtgagt gccgtcggca cggtgactgc gaggcggtcc
    11941 caggggatgt gcggattcgg atcgcggaaa tggaggctcg gcgggatcgc gccatggtgc
    12001 agcgcgagcg cggtcttcat cagcgcggcg acgccggccg cgctctccag atggccaacg
    12061 ttgctcttga ccgagccgag aagcagaggt tcagtgcgct cgcggcagag cacttcgccc
    12121 accgccatcg cctcgatcgg gtcgccgagg gatgtcccgg tgccgtgcgc ctcgaggtac
    12181 tggatctgct tcggcgaaac accggcggcg tcaagcgctt cacggatcag cgtcttctgc
    12241 gcggtgccgt tgggcgcggt gaggccgttg ctccgcccgt cgtggttgac cgcggagccg
    12301 cggatgaccg cgtaaatgcg attgccatcc gccaccgcct cggagagccg tttcagcacg
    12361 acgagaccgc atccctcgcc gcgcacgtac ccgtcggcgc tggcgtcgaa cgacttgcag
    12421 cggccatcgg ccgccatcgc gcgcatccgg cagagtccga tcgtcacctc cggcgcgaga
    12481 atcagattca cgccgccggc gagggcgaga tcgcattcgc ggttgcggag cgcctgacac
    12541 gcgagatgcg ccgtcagcag cgacgacgag caggccgtgt cgagttgcat ggccggaccc
    12601 tgcaggttga ggacatatgc gagacggccg gcggcgagac cgcgcaacag gccgagcgtg
    12661 ttgtacgcgt caatgtcgtt gcggtcggcg gcgaagaagt tcgactgcag gtagtcgtcc
    12721 atgaacgagc cgacgtacac gccggtccgc ggcgccttgt cggggacgat gccggcattc
    12781 tcgaacacct cccacgccac ctcgagcagc aggcgttgct gcggatccat gttcatcgcg
    12841 tcgagcggtg agatgccgaa gaattccgcg tcgaactgat cgatgtcacc atccatccca
    12901 tcaaggaagc cgccgtgacg cgtgtacatc ttccctgcgg cgtcgggatc ggcgtcgtag
    12961 agtgcgtcga cgtcccagcg cgagcgcgga acttcggtga tggcgtcggt accgctgcgc
    13021 agcagttccc agaacgccgc gggatcgttg gcgcggccgg ggaagcggca gcccatgccg
    13081 acgatcgcga tcggctcacg gttggcgcgc ttcgcctccg ccagcccgga gcggagactc
    13141 ttgatctcgc gcagcgcctt cttcagcagc gcgccatggt cgtccgaggg gctcatgcgc
    13201 taatcaggct cggaagtgtc cccgccggcc agctcggcgg cgagcaggcg cgccacttcg
    13261 tcgtcggcga gattgtcgat gtcgtcgatg tcgccaatct tcgcgagcac ctcgagcttc
    13321 gccgattcgg cggtgtactt caccagcgca tcgatgttgc cgtggttcca gaccagcacg
    13381 tcttccatcg gccggccgat ccactcgccg agctgcgcgg agagtttcag ccccgtcagc
    13441 gacgtcatgc cgaattcgaa gaatgagcgg tccgcggcga tcgtgctgcc gtcgacttcg
    13501 aggttgtcgg tgatccactc gcggatccac atgtcgatct cgagcgctcc cacggcgcgc
    13561 gccgtcgccg ccactggaaa tgcggtgcgt ccctcatcgc gcagcacgcg cttcaggatc
    13621 tttccggtcg ggctcttggg gagggcttcg acgaaacgga tcgccgacgg gatcttgtag
    13681 tccgcgagct gctgccggca gaaggccatg atctccgcct ctgtcgccga gtgctctttc
    13741 ttgcagacga tgttcgcgca gacgcgctcg ctcatgacct ggtcggggat accgtagacc
    13801 gcgacttctt cgaccgccgg gtggaggtag atcgtgttct cgacctcgac cggatagacc
    13861 ttgaggccgc cgacgttgac catgtccttg agccggtcga cgatgtagaa gtagccgtct
    13921 tcgtcgacgc ggccgatgtc gccggtgtgg aaccagccgt cgcgaatcgc ctgcgcggtc
    13981 gcctcggggc ggttccagta gccgagcatc acgttcggcc cgcggacgac gatctcgccg
    14041 ggatcgcccg tcgccacttc ggctccggtg tcgggcgaaa cgacccgcat ctcgacgttc
    14101 tcgatcggtg cgccgatcga gccgagcttg taccagaggc ggtggttgta gctcgcgaac
    14161 ggcgatgtct cggtgagacc gtagccttcg ttgatcggcc ggcggtactt ctcctcccac
    14221 ttgcgcgcga cctcgcgcgg aagcgtcgcc gccgcggaga agtagtagcg caccgactcc
    14281 agctcggcgg ccgaggcgag gttgtagagc gggatgaatg tcgtcggcac gccgaagaac
    14341 atcgtcacct gatcgtcggc gagcgagcgg atgatcgtgg cgggatggaa ggtccggtgg
    14401 aggacgaccg tcgcgcatgc gttgaagccg ctgttgagga tcgcgttctg cccgaagcag
    14461 tggaagagcg gcaggaacag cagaagcttg tcgtccaccc gcatgccgca gttgtagttg
    14521 aacgcgtgca tgttggagat cacgttgccg tgcgacaggc aggcaccctt cggctctccg
    14581 gtcgttccgg acgtatacag aatggccgag ggatcgtcgc ggtccatgat cgctggggtg
    14641 aattcgccgg tcgtattcgc cgtcaaatcc gagagcgagg tatcggtgcc ggatgccgcg
    14701 ccttcgacga tgactacgag cttcagctcc ggcgacgcgg cgcgatcgac gtgcccgcgc
    14761 aatacctccg tcgtgaacag cgcaaccgcg ccgctgtcat tgagaatgaa gcgggtctct
    14821 tccgtccgca gcgtcgagtt cagcgacacc gccacggcgc cgagcttctg aatggccagg
    14881 taggcggcgg cgaactccgg aatgttcgga aggaacagcg ccacgcgatc gccgcgtccc
    14941 acgcccgatg cagccagtcc ctcggccaca cgattcgacc actcgtcgag atcggcgtac
    15001 gtgtagtggc gattctcgaa cgtcagtgcg actttgcgcg ggaacagctt gcggccgcga
    15061 atgatgttct gcgcaacatt catctctacc tgttcctcct ggcgcgagac gagggtgatt
    15121 cccggctcct tgagtgcctg cagcgtgtcc tgcagcgcgt tcggaatggc gccgtcgccg
    15181 gcgatgtcgg cggcgagctc ctcgatgtag atgtcgagcg actgatcgcg gtcgtcgtgc
    15241 acgaacgcgc gctcgcgccc cggcttgacg cggcagatgc tgcagaccgc ctcgtacagt
    15301 ggcaccgtcg ccggcgagag tccggcgcgg ccgtcgtaat gtccctcttc gcggtgcgcg
    15361 cgcagatcgg cggcctggac accgaacatc agcgccaccg ccagatactg ctgcgagaac
    15421 gacaccgcgc gccgcgcgag attcgccgag ccgaaaccca aactgttgac gttctgattg
    15481 aactgctcgg cgtgcgtcag gtagcgatcg gccatcggag aggcgagatg cagcaactcc
    15541 gggacgatcg agttcccggt cagttgcaac cccttgagcc cgttattcac gctgcgcgct
    15601 tcgttgccga cgagcgacga cgggagaccg ttgttgaact ccggcgccac cagcatcgcg
    15661 atctgcgtgt cgagatgttt cgccgtcagc gccaggtact gccgcaactg gtccatgccc
    15721 acgccgacat actggccgag gaagttgccc ccttcgtagt acgactcgcc ttcgccgtcg
    15781 aagagcggat tgtcggtggc ggaattcgct tcggtctcca tctgccgggc gattacggcc
    15841 agtccgtcga cgaccggtcc gaggtactgc ggaatgcagc ggagtgaata gcggtcctgc
    15901 gccaggctcc cagcggaatg ctcgaagccg ccgcgggtct cgccgtacgt ccacttcgag
    15961 cccttgagca gcttgaagag attccgcgcc acccacacct ggccgggatg cggcttgtga
    16021 tggtggatga atggatgaag cgcatgcgcg ttgccgcgca tcgcctggaa catcagcgcg
    16081 tgcgcgccca tcgccagcgc gatgaccacg cggctgtcgt agaccgcgtt catggcgatc
    16141 gcggtcatga ccgaggtgcc gttgacgaga gccagacctt ctttcggcgc catgcgctcc
    16201 ggctccagac ccagggtgcg gagcgcatcg atcgcgccca tttccttgcc gcgaacatcg
    16261 actttgaatg agttgtcgag accgacgatc gcaccggcga tggcggcgag cgggacgagg
    16321 tcgccgctgg cgccgatcga accgagctcg cgcacgtgcg gggtcaggcc gttgttcagg
    16381 aacgtctcga accggcgaat cagctccatc cggatgcccg acacgccgca caacagcgag
    16441 ttcgcgcgca gcagcatgct ggcacggacg tcgccggccg gcagtctgcc gccggctccc
    16501 gacttcagga accagagcag tgcttcctgc agatcgcatg catccccggc cgtgatggcc
    16561 gtgttcgatt tgccgccgaa tcccgtcgtc acaccgtaca gccgctcgcc cgcggcgatc
    16621 agccgggtca catactcctg cgagccgcgg accttctgtt cgacgcgcgc gtcggtggtg
    16681 atgctcacct tcgcgccgcc gcgcgccacc cgcgcgacct cgtcgatcgt caggtctttc
    16741 ccgcgcagaa tgacggtgtc atcgaccatg aacgcttacc agttgaaggg accagtgatc
    16801 tcgtcggtct tgtcgccgcc ggggaccgtg tagtgcagga ccgccgaaaa gcgcgtcaac
    16861 gcccggttct tgatcttgtc gccgccgtgg atcatcaagc cgtgcgtcag gatcgcgtcg
    16921 cccttcttca ccggcagaga aacccgctgg ccgccggcga cggcatcctt tagatactgt
    16981 tcttccttcg atctcgtatc gaggtggcag gtgcggagat tcgtctgcgg gtagttgttc
    17041 cagcccggcc agatcggtac gcggtgcgat cctgggaaga caccgagggg tcccgcgagc
    17101 ggatcgacgt cctcacccgc cagccagacg ccgacgagcc ggttcttcgg atggatgtgc
    17161 atcgccgcga gatcctgatg cacgccgacc tcgctgcccc acttgaagcc gatcgaactg
    17221 gccagcaccg cgggctcttc gagaatcaga gtggccatgc gcaggaggtg acgggacatc
    17281 gtgagcgcca gcatcgcctc cgagtgacag tgcaggtaat ggacgcgcca gagctgctgg
    17341 tcgcgcagct cgaggcggta ctcgagcggc cactgcagca gctcggcgtg acggatcgga
    17401 ccgggcggtc tgcccttgat gtgcaggctc atgatatgca gcccggggat gacctggtcg
    17461 gtggtccaga ggtcgtcgag gaaccgattg aactgatcga tcgtgccgaa atcggactgg
    17521 tcgatcgcac cgcgcaggat gaagtagcct tcgacgaccc actgccggag cagctcggcc
    17581 tcttccgatg agatcagccc ctctgccagc cgcgactgaa tcctctcctc ggcgtcatcg
    17641 cggtcgagcc acggaacgtc attcgggtcc tgattccacg gccgtccgcg tctcgcgatc
    17701 acttcgggcg acacaccatg ccagttcggt gactcgcgca tcggctcgtt cttcgcccaa
    17761 aactccgtct tcggttcgtg ccaggcagtg atggtcatca gtgtctcctt gtacacttcg
    17821 tcacgtcgcg cagcggggac tcagcacttg tctttttttg cggagaggtt acccttattt
    17881 ggctgccgag tataccgctc ggaaggcact ccgggaccgg ggagaaacgc atgatcgcat
    17941 tgttttcgcg ctggaagctg aaggacggat gtccgcctca gttcgctgca gcactcgata
    18001 cgctgaccaa ggccgtccgc gcgcaggagc cggggacgtt gatgtactcc gtccacgtcc
    18061 cggggccgca tccacccatc ggtccgccgc cggaatatga g
//
LOCUS       DATLVW010000405         9239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_387718, whole genome shotgun sequence.
ACCESSION   DATLVW010000405 DATLVW010000000
VERSION     DATLVW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387718"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VNN25_12160"
     CDS             complement(<1..974)
                     /locus_tag="VNN25_12160"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXH92326.1"
                     /translation="MIENTSSMDRAVGPSSAFTRPRMIAAGVLLAVVVGLAFAFPAIR
                     RWSRADRAVDASTLSMGVVVRGDLVRDISVQGRVVAALHPTLVSPAQGIVSLRTKAGS
                     VVRRGDVLAVVDSPELRSALDQSRAQVLSLGTDYERAKIAGSQSDARLRQQVALAEVR
                     LEAAKRGLQRAELLRHDGLINGTDFERAQDDVHVAQIELEQARREIGLNRETADFDIR
                     SRKLQADRQQSATDELQKRFEDLTIRAPFDGMVAAITVNERDAVAANQAILTVINLSS
                     LELEITIPEEYAGDVRIGTPLMIGYGGGEKGAHITAVSPEIVGNQITAR"
     gene            complement(1228..1677)
                     /locus_tag="VNN25_12165"
     CDS             complement(1228..1677)
                     /locus_tag="VNN25_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92327.1"
                     /translation="MEILRALRETMPAIVPPDPLQEGRMRVAKAVPRPFVEAAINGLT
                     ASQVWALSAATTPDELKSHRDFEEQRPVLDELKAYTDILTYGLDYHHWQATAMARRAY
                     KVGKQLGGDEGLVLQPHIDIMASTLPRRSRKAKTPPKTTPPPKPVTE"
     gene            complement(1845..2120)
                     /locus_tag="VNN25_12170"
     CDS             complement(1845..2120)
                     /locus_tag="VNN25_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92328.1"
                     /translation="MSVTVRPVDATAHTVSITKQLVNATSHWISAIPKAFAGTVKRST
                     LTVACYAPSRGKPRDVQLGTAVYKKDYRALLNTRGGETFSFLMNLKE"
     gene            complement(2586..3923)
                     /locus_tag="VNN25_12175"
     CDS             complement(2586..3923)
                     /locus_tag="VNN25_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HXH92329.1"
                     /translation="MRIAAIAALLFSFAASSFAYQESAWIPPWNADALKSLQSNLGTL
                     NETNPVWYSWNADGTPLKNWNAENATWRSSMSGTLVPTVQNVVNGSFDGNTAAAVLAS
                     PVSRESTAAAISQLVIMQAFDGIDIDYERLPAASRDNFTAFLTTLGSKLHAANKKLSV
                     TVYAKTSDSANWNGPGGEDWRAIGGIADSVKIMAYDYSYSGSAPGPIAPLNWIDGVAT
                     YAQSVIPNDRIMIALPWYGYDWSSGAATDVTYASAMQLAQSNGATVSHDANGEATFTY
                     GGHTVFFQDASSYAKKVQLLKDRHPSIAGFAAWSAGVEDPDIWNVMRGSSSSGAAPPP
                     APAASIAISGPGSLSVSRGSSTAADYQLVAVNGFTGTAAVALQPPSGFNGTVSANAAS
                     VAAGGSIRVTVNTTSLTTPGSYQFQVNLTSGTLTATQNVTIQVNDLPRRHSAK"
     regulatory      complement(3991..4066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4189..5868)
                     /locus_tag="VNN25_12180"
     CDS             complement(4189..5868)
                     /locus_tag="VNN25_12180"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXH92330.1"
                     /translation="MNGSTDVRSSKSSQGYEPAPPTILKTDKELPLGDPIVEPTADPA
                     TASEAKNADCERCISGIDLLDYGAGGLMPNKVYLVKGAGGVGKTLFGLQFLTRGLEHQ
                     EPGILITDQKPENVLAQARGIGFGIDEAVKRGQLAILNPSNRYFELVESPADVMAIVG
                     ELGDLIRKMNARRLVIDPVYTLINTSFSSHFATSITQSLINALEDLPVTTLLVGEEDD
                     PELNAITRQLEHNAAGVVSLTHDAATGGRLMRASNLRYASNDDLTSHYRILNGRGLIN
                     YRGEDERTVDVTKPWETSRETSRKILVLGASQETLRRVQESLGNEYEVQSEPDLKAGV
                     ERVKRERPGLVLVTPSRSVTAIAAILELAHSSTSSIAFLSPNAHRQSERVLYLRAGAD
                     DFITEPFSPSELRARVDALIRRSGRRLNLRGSKLHSITPDEMSSLMSAADNPSDSKRG
                     PVLKAKSGSVDFDPDFNERLQRNVDTVRKLDQPFALYWIKADEKDADLNRDLAKLCRQ
                     EDIVCHNRDGEFVAILTGTDQNGVKGFESRLNEKLGTRLRDTEHGYELHNA"
     gene            complement(5954..7090)
                     /locus_tag="VNN25_12185"
     CDS             complement(5954..7090)
                     /locus_tag="VNN25_12185"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXH92331.1"
                     /translation="MAPDNDLDSPRLRHTRWQNPDGSGRFVRANAGPVKPTRTQTVVE
                     LMMFVVAGVLITLALIAVYTSFSPSHRAVPNRVAEGWAQDRVNIVLFGVGGANHPTHD
                     NLADSIMLLSLKPSTKQAAIVSIPRDLWVPIEGHGSHRINYAHAMGDMGGYPGAGPGL
                     LCDTVSQVFNQPVHAYVRVDFAAFEKLIDNLGGIDVYCQHGFYDYLFHDGFAQGWHHL
                     NGKRALAYARYRYVIGSEGDNYARELRQQQVISALREKLQRMQPQQALGVVEALTTLS
                     SAADTNLTTPQMISLYRAFHDIKPEEIRHVSLKPLTEPFEVTRLSEPGEAVRTHTGSF
                     QDLQKLEASIFASEQQVSTSDQIQFAATPPPPVQRAQAPRQATD"
     gene            7166..7990
                     /locus_tag="VNN25_12190"
     CDS             7166..7990
                     /locus_tag="VNN25_12190"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HXH92332.1"
                     /translation="MKAKLLVFVLLLCATAALAGDWTSIVPGLDYQEFAEGNIDVYVA
                     RVDLSNDDLMVISTRQTDKGMKVSDFAKKNDALVAVNGDYFDDHFNPIGLAIGPCGQW
                     VGSKDTSREGVIAIGEHRAEIRPQSEVMDPPEDWIETALSGWPLLVKNCTPLSASLPG
                     SAGFTRSPHPRTAAGVSKDGKTLYLVVVDGRRANVPGMTLAELAAFMNHRLDACWAMN
                     LDGGGSSAMWVEDHIVNHPSDGVERPVGDHLAVVLKSDYAGCDSPAATTTATPSQH"
     gene            8093..>9239
                     /locus_tag="VNN25_12195"
     CDS             8093..>9239
                     /locus_tag="VNN25_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92333.1"
                     /translation="MRLARSFLAVALTIMSATVLLAQQQDTSYTRITTLESAVTRGQA
                     TRAEKLELAGLYNQAGRYYEASKLTDALLASDPNDAEAKAAADVAANGRRDTAARNVA
                     AAEAKAHANSATDQDRLALADAYFDAGSYGTAASIYEHLPQAMRTRDVRLREARALAW
                     SNQTDRAERAYSALLSEQSTPDLRLEYGRVLTWMGNSHAAITTLNDVYTTAPSEDAVI
                     ALANARAWSGDREGAIALLNEYYAAHPEAAQAHDLSAQFSASPDLRLERVGKLIAAQP
                     YNLALHVERARMLYDAGRYAEARNEVTFVREHSRAKIAGLDDLAAQIEQRRHEELAKL
                     DQRRAALDAQAATAMASSSVNDADELLSLAKAYTGLGAYDQSISLYNR"
BASE COUNT         1737 a         2903 c         2780 g         1819 t
ORIGIN      
        1 cgcgccgtga tctggttgcc gacgatctcc ggcgagaccg cggtgatgtg cgcgcccttc
       61 tcgccgccgc cgtagccgat catgagcggc gttccgatcc tcacatcgcc cgcgtactcc
      121 tcggggatcg tgatctcgag ctcgagcgac gaaagattga tgacggtgag gattgcctgg
      181 ttcgcggcga cagcgtcgcg ctcattaaca gtgatcgctg ccaccatgcc gtcgaacggc
      241 gcgcggatgg tcaggtcctc gaagcgcttc tgcagctcgt cggtggctga ttgctgcctg
      301 tcggcctgca gtttccggct gcggatatcg aagtcggccg tctcgcgatt gagtccgatc
      361 tcgcgtctgg cctgttccag ctcgatctgc gcgacatgca catcgtcctg cgcacgctcg
      421 aagtcggtgc cgttgatcaa tccatcgtgc cgcagcagct ccgcgcgttg cagcccacgc
      481 ttcgccgcct ccagccgcac ctcggcaagc gcaacctgct gccgcaaccg cgcatccgac
      541 tgcgagccgg cgatcttcgc ccgctcatag tcggtgccga gcgaaagaac ctgcgcccgc
      601 gactgatcga gcgcgctgcg caactcggga ctgtcgacaa ccgcgaggac atctcctcgg
      661 cgcaccacac tccccgcctt cgtccgcagc gacacgatcc cctgcgccgg actgacgagc
      721 gtgggatgca acgccgcaac gacgcgcccc tgcacggaga tgtcgcgcac aagatcgccg
      781 cggacaacaa cgcccatcga tagcgtggag gcgtccacgg cgcgatcggc gcgcgaccac
      841 cgcctgatgg ccggaaacgc aaacgccagc ccaacgacaa ccgccagcaa cacccccgcc
      901 gcaatcatcc tcggccgcgt aaacgccgac gaagggccga ccgcgcggtc catcgaggat
      961 gtgttttcaa tcattcagcg acgctacagg gcaaccgagg tgccatgagc gaagtgattg
     1021 attctccgtg tgatccgcgg atttggctat gtccgaggag tgtccgaggg gacatgtgtc
     1081 cgcggacagg tgtccgggta acgtggagaa gcttgatcac cacaaagaca caaagagcac
     1141 aaaggttctt tgtgtccttt gtgcctttgt ggtgaatggt tcaaaagtgg cggccccaaa
     1201 cagaagaccg ccacttgcac aacattacta ctcggtcaca ggcttcggtg gcggtgtcgt
     1261 cttcggagga gtcttcgctt ttcgcgacct tcgaggaagc gttgacgcca tgatgtcgat
     1321 gtggggctgg agaaccagac cttcatcgcc gccgagctgt ttgccgacct tgtacgccct
     1381 gcgcgccatc gcggtggcct gccaatggtg ataatccaga ccgtacgtga ggatgtcggt
     1441 gtacgccttc agctcgtcga gtacaggacg ctgctcttcg aagtcacggt gcgatttcag
     1501 ctcgtcgggc gtcgtcgctg ccgatagtgc ccacacctgg gatgccgtga gaccgttgat
     1561 ggccgcttcg acaaacggcc gtgggactgc tttcgccacg cgcatccgcc cctcctgaag
     1621 cggatccggt ggcacaatgg ccggcatcgt ttcccgcaac gctctgagga tctccacgcg
     1681 gtctgcggga gccagattcg tcgcgcggat tgtcgctacg atgtctgccg gggttaacgt
     1741 tactgcggtt gtttcactgg aactaccggg ggaaagggac atgggtctcc tttactttgt
     1801 tatggactaa acatgcgagc tgtcgcctat tcgccagttc ctcgctattc ctttagattc
     1861 attagaaatg aaaaggtttc tccaccacgc gtgttgagca atgcgcggta gtctttctta
     1921 tagacagcgg tccctaattg gacgtcgcgg ggtttccccc gtgacggcgc gtaacaagcc
     1981 acagtcagcg tgctccgttt gaccgttccg gcgaatgctt tcgggatggc gctgatccaa
     2041 tgactggtgg cgttcaccag ttgcttcgtg atactcaccg tgtgcgcggt ggcgtccacc
     2101 ggtcgaacgg tgacgctcac tactggagcg gtgactctcc acccgttgac tggtgacggt
     2161 cacgcattga ctggtgagcg ccatgctgtg agtgatggac gcccaccgct tgactagtcg
     2221 cgcccacagt ttgactggtg acagtccacc agttgactgg tgacggggac gagttaactg
     2281 gtggactgtc accactcaca gcgtggcgct cgccactcac agcgtgacgg ccaccactca
     2341 caacgtgtac gcccaccact cgcagcgtgg cgctcaccac tcgcagcgtg gcgccgacga
     2401 gttcctgcgt gacgcgcacg ctatcgagcg tccgcgtgac gctatcaatc aagcgccgaa
     2461 ccagtgcggc tttcaccgcc gcgcacgtga gttcaacggc ttatccgcag atatcgcaga
     2521 tgttcacaga taggatctgc gtctatctgc gtcatctgcg gatggcttct gcgagtgatc
     2581 gcagactact tcgccgagtg ccgccgcgga agatcgttca cctgaatcgt cacgttttgc
     2641 gtcgcggtga gcgtgccgct cgtcaggttg acctggaatt gatagctgcc gggcgtcgtg
     2701 agcgacgtcg tattgaccgt gacgcggatc gatccaccgg cggccaccga cgcggcgttc
     2761 gcgctgaccg tcccattgaa gccgctcgga ggctggagtg ccacagccgc cgtgccggtg
     2821 aatccattca ccgcgacgag ctgataatcc gccgcagtcg acgatccgcg gctcacgctg
     2881 agcgatcccg gacccgaaat ggcgatcgat gcggctggcg cgggtggtgg tgcggcgccc
     2941 gaggagctcg atccgcgcat gacgttccag atgtcgggat cttccacgcc ggcgctccag
     3001 gccgcgaagc cggcgatcga tgggtgccga tccttcagca attgcacctt cttcgcgtag
     3061 ctcgaagcat cctggaaaaa caccgtgtgg ccgccgtacg tgaacgtcgc ttcgccgttc
     3121 gcgtcatgcg acaccgtcgc gccattgctc tgcgccagtt gcatggccga ggcgtaggtc
     3181 acgtcggtgg cggcgccgga gctccagtcg tatccatacc atggcagtgc aatcatgatc
     3241 cggtcattcg gaatcacgct ctgtgcatac gtggccacgc cgtcgatcca gttcagcgga
     3301 gcgatcggtc ccggcgcgct gcccgaatag ctgtagtcgt acgccatgat cttgacggag
     3361 tccgcaatgc cgccgatcgc acgccagtcc tccccgcccg gaccattcca gttggcgctg
     3421 tcgctggtct tcgcgtagac ggtgacggaa agcttcttgt tcgccgcgtg cagtttcgat
     3481 ccgagagtcg tcagaaacgc cgtgaagttg tcgcgcgacg ctgcaggcag gcgctcgtaa
     3541 tcgatatcga ttccgtcgaa ggcctgcatg atgacgagct gcgagatcgc cgcggccgtc
     3601 gattcacgtg agaccggcga cgccagcacg gccgccgcgg tgttcccgtc gaaactgcca
     3661 ttgacgacgt tctgcacggt cggaacaagc gtgccgctca tcgatgagcg ccaggttgcg
     3721 ttttccgcgt tccagttttt caacggtgtt ccgtcggcgt tccagctgta ccagaccggg
     3781 ttcgtctcgt tcagcgtgcc gaggttcgat tggagggatt tgagggcatc ggcgttccag
     3841 ggtggaatcc acgccgactc ctgataggcg aacgatgaag cggcaaatga gaacagcaac
     3901 gcggcgatcg cggcaatgcg catcaggctc cttcgccctg cgatccgcta tgccgttctc
     3961 caatgcggag aggaacgttc cagaatcacg ttcggcagtc cagccgtctg ctatcagacc
     4021 tgagactttg cgtcctcccg tcgccgggag tatgcctttg tcgcgcgatc gcggcttttc
     4081 ttaagactca caataataga ttgcgcgcgc gtgccattcc acctcgacca taaacaaaag
     4141 agcggcctga gccgctcttc tgatgtgtca gctaatgatt cgattcaatc aggcgttatg
     4201 cagttcgtat ccatgctccg tgtcgcgcag gcgcgtgccg agcttctcat tcagccggct
     4261 ctcgaaccct ttcacaccgt tctggtcggt tccggtgagg atggccacga actcgccgtc
     4321 gcgattgtgg cagacgatgt cttcctgccg gcaaagcttg gccagatcgc gattgagatc
     4381 ggcgtccttc tcgtccgcct tgatccagta gagcgcgaag ggctgatcga gcttgcggac
     4441 cgtatcgaca ttccgctgca gacgctcgtt gaaatccgga tcgaagtcga cgctgccgct
     4501 tttagctttc aataccggac cacgcttcga atcactcggg ttgtccgccg cgctcatcag
     4561 tgacgacatc tcgtcaggcg tgatcgagtg cagcttcgat ccccgcaggt tgagccgcct
     4621 gccgctgcgc cggatcagcg catcgacgcg tgcacgcaac tcggagggtg agaacggctc
     4681 ggtgatgaaa tcgtccgctc ccgcgcgcag ataaaggacg cgttccgact gacggtgtgc
     4741 attgggcgag aggaacgcga tcgatgacgt ggagctatgc gcgagctcga gaatcgcagc
     4801 gatggcggtc acggagcgcg acggcgtcac cagcacgaga ccgggccgtt cacgcttgac
     4861 gcgctcgaca ccggctttga gatccggctc cgactgcact tcatactcat tgccgaggga
     4921 ctcctgaacg cggcgcagag tctcctggct cgcgccaaga accaggatct tgcgcgaagt
     4981 ctcgcgcgac gtttcccacg gcttggtcac atcaaccgtc cgttcgtcct cgccgcgata
     5041 attgatcagg ccgcggccgt tcagaatccg gtagtgcgat gtgagatcgt cattgctggc
     5101 gtagcgcaga ttggacgcgc gcatgagccg gccgccggtg gctgcgtcat gagtcagcga
     5161 cacgacgccg gccgcgttat gctcgagctg tctcgtgatg gcgttcaact ccggatcgtc
     5221 ctcttcgccg acgagcagcg tcgtcaccgg cagatcttcc agcgcattga tgagcgactg
     5281 tgtaatcgat gtcgcgaagt gactggagaa cgacgtgttg atcagcgtgt agacaggatc
     5341 gatgacgagg cgccgcgcat tcatcttccg gatcaggtcg ccgagctcgc cgacaatggc
     5401 catgacgtcg gccggactct ccacgagctc gaagtagcga ttgctgggat tgaggattgc
     5461 cagttggccg cgcttgactg cctcgtcgat gccgaagccg attccgcgcg cctgcgccag
     5521 gacgttctcc ggcttctgat cggtaatcag aatccccggt tcctgatgct ccagtcctcg
     5581 ggtaaggaac tgcaacccga acagcgtctt gccgacgccg cccgcgcctt ttacgagata
     5641 aaccttgttc ggcatcaggc cgccggcgcc gtagtcgaga aggtcgatgc cgctgatgca
     5701 gcgctcgcag tcggcgttct ttgcctcgga ggcggtagcc ggatcggcgg tcggttcgac
     5761 gatcggatcg ccgaggggaa gctctttatc ggttttgaga atggtgggag gggcgggttc
     5821 ataaccctgg gatgatttgc tcgatcgcac gtccgtggat ccgttcataa gaagcctctt
     5881 ctctcaatga gtgggttgta cagctcgtgg tttcttacgg cagagcagcg agaaagcaat
     5941 gactgcgcca ccgtcaatcc gtcgcctgcc tcggcgcctg cgcacgctgc acaggcggag
     6001 gcggtgttgc tgcaaactga atctgatcgc tggtcgatac ctgttgttcg ctggcaaaga
     6061 tcgatgcttc cagcttctga agatcctgga agctgccagt gtgggtgcgt accgcttcgc
     6121 ccggctcgct caaccgcgtc acctcgaacg gctccgtcaa cggcttgagc gagacatgcc
     6181 ggatctcctc cggcttgatg tcatgaaatg ctctgtacag cgagatcatc tgcggcgtcg
     6241 tcaggttcgt atcggccgcg ctcgacaatg tcgtcagcgc ctccaccacg ccgagcgcct
     6301 gctgcggctg cattctctga agcttttccc ggagcgcgct gatgacctgt tgctgccgca
     6361 gctcccgcgc gtaattgtcc ccctcggatc cgatcacata ccggtagcgc gcgtatgcca
     6421 gcgcccgctt accgttgagg tgatgccagc cctgggcgaa tccgtcgtga aacagatagt
     6481 catagaagcc gtgctggcag taaacgtcga taccgccgag gttgtcgatc aacttctcga
     6541 acgcggcgaa gtcgacgcgc acataggcat ggaccggttg attgaagacc tgcgacacgg
     6601 tgtcgcacaa gaggcccggg cctgcaccgg gataaccgcc catatcgccc atcgcgtgcg
     6661 cgtaattgat gcgatgcgat ccatgtccct cgataggaac ccacaagtct ctgggaatgg
     6721 aaacaattgc cgcctgcttc gtcgacggct tgagactcag gagcatgatg gagtcggcaa
     6781 gattgtcgtg ggttggatga tttgcgccac cgacgccgaa gagcacgatg ttcacgcgat
     6841 cctgcgccca tccctcggcc actcgattcg gcactgccct gtgcgacggt gagaagctgg
     6901 tgtagaccgc aatgagagcc agagtaatca gcaccccggc caccacgaac atcatcaact
     6961 ccacgaccgt ctgggttctt gtgggtttta ccggcccggc attcgcgcgc acgaagcggc
     7021 cgctaccgtc ggggttttgc cagcgcgtat ggcgcaagcg aggggaatcg agatcgttgt
     7081 cgggtgccat tgctgaagtg tggcgacgca ggagtttgca ttcgcggtgc cacgcgttgg
     7141 cagaccgctt gaacatgcgc cgcgcatgaa agccaaactc ctcgtattcg tacttctcct
     7201 ttgcgcaacc gcggcgcttg ccggcgactg gacttcgatc gtccccggcc tcgactatca
     7261 ggaattcgcc gaaggcaata tcgacgtcta cgtcgcgcgc gtcgacctca gcaatgacga
     7321 cctcatggtc atctcgacac gccagaccga caagggaatg aaggtcagcg acttcgcgaa
     7381 gaagaatgat gctctggtcg cagtcaacgg cgactatttc gacgaccatt tcaatccaat
     7441 cggcctggcg atcggccctt gcggtcaatg ggtgggatcg aaagacacca gccgcgaggg
     7501 cgtcatcgcc atcggtgagc accgcgcaga gatcagaccg caatcggaag tgatggaccc
     7561 tcccgaggat tggatcgaga ccgcgttgtc cggctggccc cttcttgtga agaattgcac
     7621 gccgcttagc gcaagcttgc cgggaagcgc cggctttaca cgctcaccgc atccgcgcac
     7681 cgcagccggc gtgtcgaagg atggcaagac tctctatctc gtcgtcgtcg acgggcggcg
     7741 cgccaatgtg ccgggcatga cgctggccga gctcgccgca ttcatgaatc accgtctcga
     7801 cgcctgctgg gcgatgaacc tcgacggcgg cggatcgagt gcgatgtggg tcgaagatca
     7861 catcgtcaat catccgagcg acggtgtcga gcggccggtc ggtgatcatc ttgccgtcgt
     7921 gctcaagagc gactacgccg gctgcgatag tcctgccgct acgacgactg caacgccatc
     7981 gcaacactaa actggaatat ccattgcaat gcggcccacc cgagaggctg caacattctt
     8041 cagtatcacc ctccatttga caactgagat caggatcagg gaaggacgcc caatgaggct
     8101 cgctagaagc ttcctcgccg tcgcactgac aatcatgtcc gcaacggttt tactcgccca
     8161 gcaacaagac acttcctaca caagaatcac tactctggag tccgcagtca ctcgaggcca
     8221 ggccacaagg gcggagaagc tcgagcttgc cggcctctac aatcaggcag gccgctatta
     8281 cgaggcgtcg aagctcaccg acgctctcct cgcttccgat ccgaacgacg cggaagctaa
     8341 agcggcagcc gacgtcgccg cgaatggaag acgcgacacc gccgcgcgca acgttgcggc
     8401 agccgaggca aaggcgcacg ccaacagcgc gacggatcag gaccgccttg ccctcgccga
     8461 cgcgtacttc gacgccggca gctacggcac cgcggcttcg atctacgagc acctgccgca
     8521 ggcgatgcgc acgcgcgatg tgcgcttgcg tgaagcgcgt gcattggcat ggtcgaacca
     8581 gaccgatcgc gccgagcgtg cttactccgc gctcctcagc gagcagtcga cgcccgatct
     8641 caggctcgaa tacggccgcg tgctgacctg gatgggcaac tcgcacgccg caatcacgac
     8701 gctcaacgac gtctacacca ccgcgccgag tgaggatgcg gtgatcgcac tcgccaatgc
     8761 acgggcgtgg agcggcgacc gggaaggcgc gatcgccctt ctcaatgagt actatgctgc
     8821 gcatccggaa gcggcgcagg cgcatgatct ctccgcgcag ttctccgcct cgcccgacct
     8881 gcgtctggag agagtcggca aactgatcgc cgcgcagccc tacaacctcg cccttcacgt
     8941 cgagcgggcc agaatgttgt acgacgcggg aaggtatgcc gaggcgcgta atgaggtgac
     9001 cttcgtaagg gagcactcac gcgcgaagat cgccgggctc gatgatctcg ccgcccagat
     9061 cgaacagcgc cgccacgagg agctcgccaa actcgaccaa cggcgcgccg cactcgacgc
     9121 gcaggcagcg accgcgatgg cctcatcgtc cgtcaacgac gccgacgagc tcctctcact
     9181 tgctaaagcc tacacaggcc tcggcgccta cgatcaatcg atcagccttt ataaccggt
//
LOCUS       DATLVW010000406         2885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_427976, whole genome shotgun sequence.
ACCESSION   DATLVW010000406 DATLVW010000000
VERSION     DATLVW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427976"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VNN25_12200"
     CDS             <1..1387
                     /locus_tag="VNN25_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH92334.1"
                     /translation="PRSSGVHGDFESGGHGLRNAALSGDYAAESIQAAGSYEHRADDG
                     FNPNDDFHQNSASGNVKWSLSDAFSLGAAARHTSYNLGIPFNTNAAGTALVPSLDRRQ
                     NGTETQIAVPVTLTLGRFISDLTLSDNHRRDDFSDPEDPFTTSASTDAKTRRARLTTR
                     FASPAGTIVAGGEYQRDVVNDVTNFGPNFLDRRRRDNSLFIEDRYSHPFSQTSHLELS
                     AGVRRDRFDTFGSQTSPRIAAAIVNGSTKWRAAYGTGFRAPSLGELYFPFFGNAALKA
                     ESNRSYEIGYDAAMGRDGLFSLTYFNSHYRNLITFDPVTFISQNIGRVRADGIELGAT
                     QHVSANVYTAFSYTYLHRDEDETTGLRLLRRPKHSGSLATGYRSGAVDTNLVILRTGE
                     REDSMAVLPFGRVTNRSYTTLDLNVQYRLSRLTPYVKLENLTNARYEEVVGYASPARR
                     AIVGVRFSTSR"
     gene            1396..1920
                     /locus_tag="VNN25_12205"
     CDS             1396..1920
                     /locus_tag="VNN25_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HXH92335.1"
                     /translation="MKMTFTPRFWLSLALVVAGVVLRIIPHPWNFAPVGAIALFAGAH
                     FERRSWAIAVPLLTMLAGDAIIGFHSLMPFVYLSYALIAAIGMLLRGRRTSLPAVGGG
                     FLVSSTIFYLITNFAVWMMGTMYAKTAAGLVACYVAAIPFYGNTLASDAIYTAAFFGI
                     FAVSERQIPMFART"
     gene            1917..2459
                     /locus_tag="VNN25_12210"
     CDS             1917..2459
                     /locus_tag="VNN25_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92336.1"
                     /translation="MTPRIVSLLPSATEIVCALGLEHALVGRSHECDFPPGVERLPVC
                     TAPKAGGNNTRDIHENITAILQHDLSVYSVDAERLRDLRPTHIVTQIQCEVCAVSLRD
                     VEAALASWSDARPKIVALNPQSLDDVFGDIRHTADSVGATAAGENVIHSMRQRLDSIA
                     AVTRDLPEKPRVATIEWMER"
     gene            2459..2836
                     /locus_tag="VNN25_12215"
     CDS             2459..2836
                     /locus_tag="VNN25_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH92337.1"
                     /translation="MAAGNWMPTLIELAGGINLLGEANGHSPWITWNDLAEADPDVIT
                     VFPCGFRIGETIRDWDLLQSHAEWRTLRAVREDRIFVLDGNQYFNRPGPRLAESAEIL
                     AEIFHPQAFDFGHRGTAWQHIGH"
BASE COUNT          517 a          990 c          832 g          546 t
ORIGIN      
        1 gccgcgaagc agcggcgttc acggcgactt cgagagcggc ggccacggac tgcgcaacgc
       61 ggcgttgagc ggggactacg cggccgagtc catccaagcc gccggcagct acgaacatcg
      121 tgccgacgac ggcttcaatc cgaacgatga cttccaccag aacagtgcca gcggcaacgt
      181 gaagtggtcg ctgtccgatg ccttctcgct cggcgctgcc gcccgccaca cttcctataa
      241 cctcggcatc cccttcaaca cgaatgccgc gggcaccgcg ctcgtgccga gtctcgaccg
      301 ccgccagaac gggacggaaa cgcagatcgc ggtccctgtc acgctcaccc tcggccgttt
      361 catcagcgat ctgacgcttt ccgacaacca tcgccgcgat gacttctccg atcccgagga
      421 tcccttcacg acgtcggcgt cgacggatgc gaagacgcgc cgtgcgcgct tgacgacgcg
      481 cttcgcctcg ccggcaggaa cgatcgtcgc cggcggcgag taccagcgcg acgtcgtcaa
      541 cgacgtcacg aacttcggtc cgaactttct cgacaggcgc cggagggaca attccctctt
      601 catcgaggac cgctactccc atccgttcag ccagacgtcg cacctcgagc tcagcgccgg
      661 cgtccgccgc gaccgcttcg acaccttcgg ctcccagacc tcgccgcgca tcgccgcggc
      721 catcgtgaac ggatcgacca aatggcgcgc cgcgtacggc accggcttcc gcgcgccgtc
      781 cctgggcgag ctttacttcc ccttcttcgg caacgccgca ctcaaagcgg aaagcaaccg
      841 cagctacgag attggttacg acgcggccat gggacgcgac ggactcttct cgctcaccta
      901 cttcaacagc cactaccgca atctcatcac cttcgatccg gtcacgttca tctcgcagaa
      961 catcggccgc gtgcgcgcgg acggcatcga gctgggagct acgcagcacg tgagcgcgaa
     1021 cgtctacacg gcattcagct acacctacct gcatcgcgac gaagacgaga cgaccgggct
     1081 tcgcctgctg cgccgtccaa agcactccgg ctcgctggca acgggctatc gcagcggagc
     1141 cgtggatacg aaccttgtca tcctccgcac gggtgagcgc gaagactcca tggctgttct
     1201 ccccttcggg cgcgtgacca accgctcgta tacgacgctc gacctcaacg ttcagtaccg
     1261 tctctcgcgt ctcactccgt acgtgaagct cgagaatctg acgaacgcgc gatacgagga
     1321 agtggtcgga tacgcatcac cggctcgacg ggccatcgtc ggcgtccgct tctctacgtc
     1381 gcgataggag ctgccatgaa gatgactttc actccccgct tctggctttc gctcgctctc
     1441 gtcgttgcgg gcgtcgtgct gcggatcatt ccgcacccat ggaacttcgc gccggtcgga
     1501 gcgattgcgc tcttcgccgg agcgcacttc gagcgccgtt catgggcgat tgccgtgcct
     1561 ctgctcacga tgctggccgg cgacgccatc atcggctttc attcactgat gccgttcgtc
     1621 tatctcagct acgcgctcat cgccgcgatc ggaatgcttc ttcgcggccg tcgtacttcg
     1681 ttgccagccg ttggcggcgg ctttctcgtt tcctcaacga tcttctacct catcacgaat
     1741 ttcgcggtgt ggatgatggg gacgatgtac gcgaagacgg cggccggact cgtcgcctgc
     1801 tatgtcgccg cgatcccgtt ctacggcaac actctcgctt ccgacgcgat ctacacggcg
     1861 gctttcttcg gcatcttcgc cgtgagcgag cgtcagattc caatgttcgc ccgaacgtga
     1921 ctcctcgcat cgtttcactg ctgccgagtg caacggagat cgtctgtgcg ctcggcctcg
     1981 agcacgcact ggtcgggagg tcccacgaat gcgacttccc gcccggcgtg gagcggctgc
     2041 cggtgtgcac cgctccaaaa gcgggaggaa acaacacgcg cgatattcac gaaaacatca
     2101 ctgcgattct gcagcacgac ctcagcgtct attccgtcga tgccgagcgg cttcgcgatc
     2161 tgcgcccgac ccacatcgtt acgcagattc agtgcgaggt ctgcgcggtg agtctgcgcg
     2221 acgtcgaggc tgccctggcg tcgtggtcag acgcgagacc gaaaatcgtg gcgctcaatc
     2281 ctcagtcgct cgacgatgtg ttcggcgata tccggcacac cgcggattca gtcggagcca
     2341 cggcggccgg cgaaaacgtc atccactcga tgaggcagcg gctcgattcc atcgccgctg
     2401 tcacgcgcga tctgcccgaa aaaccccgcg tggcaacgat cgaatggatg gagcgctgat
     2461 ggcagccggc aactggatgc ccaccctcat cgagctcgcc ggcggaatca acctcctcgg
     2521 cgaagcgaac ggacactcgc cctggatcac ctggaacgat ctcgccgagg ccgacccgga
     2581 cgtgatcacg gttttcccct gcggctttcg gatcggggag acgattcgtg actgggatct
     2641 ccttcagtcc catgccgaat ggcggacgct tcgtgccgtt cgtgaggacc gcatcttcgt
     2701 gctcgatggg aaccagtact tcaatcgtcc aggcccgcgg cttgcggaat ccgcagagat
     2761 tctcgcggag atctttcatc cgcaggcctt cgacttcgga catcgaggaa cggcatggca
     2821 gcacatcggc cactgaaaac attgctctcg tggagcagcg gcaaggactc ggcgtggact
     2881 ttgtt
//
LOCUS       DATLVW010000407         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_144221, whole genome shotgun sequence.
ACCESSION   DATLVW010000407 DATLVW010000000
VERSION     DATLVW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144221"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1354)
                     /locus_tag="VNN25_12220"
     CDS             complement(284..1354)
                     /locus_tag="VNN25_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HXH92338.1"
                     /translation="MPVLRSVIAASGSYIPPVRVPNSDFLNHDFRGADGAPMGKTNAE
                     IVKQFESITGIQERRYVENGTVTSDIAYAAANDALTSSRIDPESLDLIIVAHNFGDVQ
                     KGGGPPDLVPSLAARVKSKLRIAKPRAAAFDLVFGCPGWIQGVITADAMIRAGDIQRA
                     MIIGADTLSRISDPHDRDSMIYADGAGATVLEARQTDAGILAHASRSDTLDHSGMLSM
                     GPSNKDEVFIEALFLKMEGRKLYKYALQTVASSMKECLDKAGIALKAVSKILIHQANG
                     KMDEAIVTALYESEKLQPPPDVMPMTISWLGNSSVATVPTLFDLIAKGRLDGQSFHPG
                     EIVLFASVGAGMHINAFAYRMP"
     gene            1635..2051
                     /locus_tag="VNN25_12225"
     CDS             1635..2051
                     /locus_tag="VNN25_12225"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HXH92339.1"
                     /translation="MAFPHVNYLAILVSGVVIFILGGLWYSVIFKKTWIALMGIPEEK
                     MKEGSGGAMPVLLFMAFLCALVQSYILAIIINHFAPFSLMRGAMVGTICWVGFAAPTS
                     FATAIFSMTKKPLWFINSAYNLVSFIAAGMILSVWR"
     gene            complement(2121..>2401)
                     /locus_tag="VNN25_12230"
     CDS             complement(2121..>2401)
                     /locus_tag="VNN25_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH92340.1"
                     /translation="LPHYEAFTTEFENWPKRIGMGYSTPAVGEVTTKQKAYADLIAGR
                     IAVAGEHTSPAWFGFMEGALESGLVAMARIANIAGIQLEPEYGDFHAV"
BASE COUNT          418 a          698 c          791 g          494 t
ORIGIN      
        1 ctgcggttac gcacatttcg gtacaacctg gtttacgtcg caagcgatag cgagatcgtg
       61 atcgtcgcgg ttgcgcacca tcgacggcgt cctggctatt ggcaaagccg catccccccg
      121 cttcgctgag accgccgaag ccgtcctcca ctgaccccat tttggaaagc acgacgagaa
      181 cctcggcgtg attccgtggg gccggacgtt tccttcgcaa tccgcggatt taccgccggg
      241 cgagacgccc atcggccgca ggcgagacgc ctgcactcct tgcctacggc atccggtagg
      301 cgaaggcgtt gatgtgcatg ccggcgccga ctgaggcgaa gaggacgatc tcgccggggt
      361 ggaaggattg gccgtcgagc cggcctttgg cgatcagatc gaacagcgtc ggtaccgtgg
      421 ccacggagct gttgccgagc caggagatcg tcatcggcat cacatcgggt ggcggctgca
      481 gcttctcgga ctcgtagagc gccgtgacga tcgcctcgtc catcttgccg ttcgcctggt
      541 gaatgaggat cttcgagacg gccttcagcg cgatgccggc tttgtcgagg cactccttca
      601 tcgagctggc cacggtctgt agtgcgtact tgtagagctt gcgcccttcc atcttcagga
      661 agagcgcctc gatgaagact tcgtctttgt tcgatggtcc catcgacagc atgccggagt
      721 gatcgagcgt gtcggagcgt gatgcgtgcg ccaggattcc ggcgtcggtc tggcgcgctt
      781 ccaggaccgt agcgccggcg ccgtccgcgt atatcatgct gtcgcggtcg tgcggatcgg
      841 agatgcgtga gagggtgtcg gcgccgatga tcatggcgcg ctggatgtcg ccggcgcgga
      901 tcatggcgtc ggcggtgatg acgccctgga tccagccggg gcagccgaag acgaggtcga
      961 aggcggccgc gcgcggcttg gcgatgcgga gcttcgactt cacacgagcc gccagcgagg
     1021 gaacgagatc gggtgggccg ccgcctttct gcacgtcgcc gaagttgtgc gcgacgatga
     1081 tgaggtcgag cgattcggga tcgatgcgcg acgaggtcag ggcatcgttc gctgcggcgt
     1141 aagcgatgtc ggatgtgacc gtgccgttct caacgtaacg gcgttcctgg atgccggtga
     1201 tcgattcgaa ctgcttgacg atctcggcgt tcgtcttccc catcggcgcg ccatccgccc
     1261 ctcggaagtc gtgattcaga aagtccgagt tgggaacgcg gaccggcggg atatagctcc
     1321 cggaggcggc aatgacagat ctcagcacgg gcatgcagag gtggatctta acaagaggtg
     1381 tgggcatctg gggagtgggg ggtggggagt ggggagtttg ttgtcgggag tcgggagtcg
     1441 ggagtcgggg gtcggagcgg tggagatcag agatcagaga tataggagac cgcttagcca
     1501 tacttcgcgg aagccgcgag accgagcgac gtctatctcc gacccccgac tcgcgacccc
     1561 cgacccgcga cccccgacac ccgcacagtg gtatctttgc cgtagatccc caacgacagc
     1621 agaaggaggc tcccatggct tttcctcatg tgaactacct ggccattctc gtcagtggtg
     1681 tcgtcatctt catcctcggc ggactctggt actccgtgat cttcaagaag acgtggatcg
     1741 cgctcatggg tatccctgaa gaaaagatga aagagggatc gggcggcgcg atgccggtgc
     1801 tcctcttcat ggcgttcctc tgcgcgctgg tgcagtcgta cattctggcg atcatcatca
     1861 atcatttcgc gccgttctcg ttgatgcgcg gggcgatggt gggaacgatc tgctgggtcg
     1921 gattcgcggc gccgacgagc ttcgccaccg ccattttctc gatgacgaag aagccgctct
     1981 ggttcatcaa ctccgcctac aaccttgtct cgttcatcgc cgcggggatg atcttgtcgg
     2041 tttggcgatg agcctcaatt gacaattgac aatggacaat tgacagtgaa ggcgtcggct
     2101 ccgacacccg acacccgagc tcagaccgcg tgaaagtctc catactccgg ttcgagctgg
     2161 atcccggcga tgtttgcgat gcgtgccatc gctacgagtc ctgactccag cgcgccttcc
     2221 atgaagccga accatgctgg tgatgtgtgc tcgccggcga cggcgatgcg gcctgcgatc
     2281 aggtcggcgt acgctttctg cttcgtcgtc acttcgccga ccgcgggggt ggagtacccc
     2341 ataccgatgc gcttcggcca gttctcgaac tcggtggtga acgcttcgta gtgcggaagg
     2401 a
//
LOCUS       DATLVW010000408         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_99680, whole genome shotgun sequence.
ACCESSION   DATLVW010000408 DATLVW010000000
VERSION     DATLVW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99680"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..509
                     /gene="rpsG"
                     /locus_tag="VNN25_12235"
     CDS             39..509
                     /gene="rpsG"
                     /locus_tag="VNN25_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXH92341.1"
                     /translation="MPRRREVPKREILPDPVYNSQLVTKFINSLMSQGRKSVAERVFY
                     SALKKVEDRAKDDPIKLFKKAVDNVKPALEVKSRRVGGSNYQVPIEVRPSRRTALAIR
                     WLISYANSRGEKTMMDKLAGEIMDAANNRGNAIKKKEDTHKMAEANKAFAHYRW"
     gene            806..2896
                     /gene="fusA"
                     /locus_tag="VNN25_12240"
     CDS             806..2896
                     /gene="fusA"
                     /locus_tag="VNN25_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXH92342.1"
                     /translation="MPRQVALEKTRNIGIMAHIDAGKTTTTERILYYTGRTHKIGEVH
                     DGTATMDWMVQEQERGITITSAATTAMWGDVRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VGVFCAVCGVEPQSETVWRQADKYGVPRIAFINKMDRIGANFEAVVNQIRTKLGKNAV
                     ALQLPIGAEDKFRGVIDLVQMKALIFNDESLGAKYETEEIPAEYREEAEHLREKLVES
                     VAETHDELLEKYLAGEPIELSELKAAIREATIAQKITPVMCGSAFKNKGVQPLLDSVV
                     DYLPSPVDIPPVKGIDPDDESLIERKASDSEPFAALVFKIMTDPFVGQLAFFRVYSGH
                     VESGTSVLNTTKGNSERIGRLLQMHANKREEIKEVWAGDIGAAVGLRSVTTGDTICDR
                     DKPIVLETMTFPEPVIAVAIEPKTKVDQEKMGVALAKLMQEDPTFKVHTDPETNQTII
                     RGMGELHLEIIVDRMVREFGVAANVGKPQVAYREAITRKSEAEGKFVRQSGGKGQYGH
                     VKMRFEPIPEGGFEFLNEIVGGSVPREFIKPVEQGIKEALERGIVAGYPVTGVRAILY
                     DGSYHDVDSSEMAFKIAGSMAFQEGARKAAPILKEPIMDVEVVTPEDYAGDVMGDLSR
                     RRGRIQAMEERAGAKVIKAHVPLSEMFGYATQVRSMSQGRASYTMQFLAYEPAPKSVA
                     DEVIAKSAGTTQRI"
     gene            2967..3239
                     /locus_tag="VNN25_12245"
                     /pseudo
     CDS             2967..3239
                     /locus_tag="VNN25_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT          732 a          963 c         1012 g          570 t
ORIGIN      
        1 aagtacgggg ccaagcggcc gaagaagggt taatccagat gccgagacgc cgagaagttc
       61 cgaaacgcga gatcctgccg gatccggtct acaacagcca gctcgtgacc aagttcatca
      121 actccctgat gtcgcagggg aggaagtcgg tcgctgagcg cgtgttctac agcgcgctga
      181 agaaggtgga agaccgggcc aaggacgatc cgatcaagct cttcaagaag gcagtcgaca
      241 acgtcaagcc ggcgctcgag gtcaagagcc gccgcgttgg cggatcgaac taccaggttc
      301 ccatcgaagt ccggccatcg cgccgtaccg ctctggccat tcgctggctg atctcgtacg
      361 ccaacagccg cggcgagaag acgatgatgg acaagctggc aggcgaaatc atggatgccg
      421 caaacaaccg cggcaacgcc atcaagaaga aggaagatac gcacaaaatg gcggaagcga
      481 acaaggcgtt cgctcactac cgctggtagt ttttgtgagc agttttggct agttttggtt
      541 cgtgagaatt attggtgacg ggtcaccgtt accggttttc taaggcccga agttgggcag
      601 tttcaaaccc cgattttttt aactttccgt agctccgtct cgaccggata acacagaaca
      661 ggttctgcgg ttccggaccg agcaacggga aaacaaagaa tgatcacgag gtgcgcttcg
      721 cgtctttatg cgcggggcgt acgtctgcaa tcggcgtcag ctttgccgcc gattgaccgt
      781 ctccggtaaa aaaggtaact cagtcatgcc acgtcaggta gcgctcgaaa aaacgcgaaa
      841 catcgggatc atggctcaca tcgacgcggg taaaaccacg acgacggagc gcatcctcta
      901 ttacacgggc cggacccaca agatcggcga agtgcacgat ggcactgcga cgatggactg
      961 gatggttcag gagcaggagc gcgggatcac catcacctcg gctgccacca ccgcgatgtg
     1021 gggcgatgtc cgtgtcaaca ttatcgatac ccccggccac gtcgacttta ccgtcgaagt
     1081 ggagcgttcc cttcgtgttc tcgacggcgc ggtcggcgtg ttctgtgccg tctgcggcgt
     1141 ggagccgcag tcggagaccg tgtggcgcca ggctgacaag tacggcgtcc cccgcattgc
     1201 cttcatcaac aagatggatc gcatcggcgc gaacttcgaa gccgttgtga accagatccg
     1261 caccaagctt ggaaagaatg cagttgctct tcagctcccc atcggagccg aggataagtt
     1321 ccgtggcgtc atcgacctcg tccagatgaa ggccctgatc ttcaacgatg aatccctcgg
     1381 cgcgaagtac gagaccgagg agattccggc cgagtatcgc gaagaagcgg agcatctccg
     1441 cgagaagctc gtcgagtcgg tcgcggaaac gcacgatgag ctgctcgaga agtaccttgc
     1501 cggcgagccg atcgagcttt ccgagttgaa ggcagcgatc cgcgaagcga cgatcgctca
     1561 gaagatcacg ccggtgatgt gcggctccgc gttcaagaac aaaggcgtac agcctctgct
     1621 cgactccgtc gtcgactacc ttccttcgcc ggtcgatatc ccgccggtca agggcatcga
     1681 tccggacgac gaatcgctca tcgagcgcaa ggcgtcggac tccgagccgt tcgcagcact
     1741 tgtcttcaag atcatgaccg accccttcgt cggccagttg gccttcttcc gcgtttactc
     1801 cggccacgtc gagtcaggca cctcggtgct caacacgacg aagggcaact ccgagcgcat
     1861 cggacgcttg ctgcagatgc acgcgaacaa gcgcgaagag atcaaggaag tgtgggccgg
     1921 tgacatcggc gccgccgtcg gtctgcgcag cgtcacgacc ggcgacacga tctgcgaccg
     1981 ggacaagccg atcgtcctcg agacgatgac gttcccggag ccggtcatcg ccgtcgccat
     2041 cgagccgaag accaaagtcg atcaggaaaa gatgggcgtc gcgctcgcga agctcatgca
     2101 ggaagatcct acgttcaagg tccacacgga cccggaaacg aaccagacca tcatccgtgg
     2161 aatgggcgag cttcacctcg agatcatcgt cgatcgcatg gtgcgcgagt tcggcgtcgc
     2221 cgcgaacgtc ggcaagccgc aggtcgcgta tcgcgaggcg attacgcgca agtcggaagc
     2281 cgagggcaag ttcgttaggc agtcgggcgg taagggccag tacggtcacg tcaagatgcg
     2341 cttcgagccg atccccgagg gtggattcga gttcctcaac gagatcgttg gcggctcggt
     2401 tccgcgcgag ttcatcaagc cggtcgagca gggcatcaag gaagcgctcg agcgcggcat
     2461 cgtggccggt tatccggtga cgggcgtgcg tgcgatcctg tacgacggct cgtaccacga
     2521 cgtcgattcg agcgaaatgg cgttcaagat cgccggctcg atggccttcc aggaaggggc
     2581 gcgcaaagcg gctccgatcc tcaaagagcc gatcatggac gtcgaagtcg tcacgccgga
     2641 agactacgcc ggcgacgtca tgggcgatct ctcccgccgc cgcggacgta ttcaggcgat
     2701 ggaagagcgt gctggcgcga aggtgatcaa ggcgcacgtg ccgctgtcgg agatgtttgg
     2761 ttacgcaaca caggtacggt ctatgtcgca gggccgcgcc agttacacga tgcagttcct
     2821 cgcttacgag ccggctccga agagcgtggc ggacgaagtg attgccaagt cggcaggaac
     2881 gacacaacga atttgaaccg cgtaggccgg actcaacggt caggccgacg caagaaactc
     2941 gaaacgaacc agcaggaggt ctcaggatgg ctaaagagaa attcgatcgc agcaagcctc
     3001 acgtcaacgt cggcacgatt ggtcacgtcg accacggcaa aacgaccctg acggcggcaa
     3061 ttaccaaggt cctggcttcg aagggcacgg cgacgttcac gtccttcgac atgatcgaca
     3121 aggctccgga agagcgcgag cgcggcatca ccatcgccac cgcgcacgtc gagtactcga
     3181 ccgagaagcg ccactacgcg cacgtcgact gcccgggtca cgctgactac gtgcagtcga
     3241 cgtgcgcgta gtggcgcttc tcggtcgagt actcgac
//
LOCUS       DATLVW010000409        10429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_513739, whole genome shotgun sequence.
ACCESSION   DATLVW010000409 DATLVW010000000
VERSION     DATLVW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513739"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1104)
                     /locus_tag="VNN25_12250"
     CDS             complement(145..1104)
                     /locus_tag="VNN25_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXH92343.1"
                     /translation="MLDDLHALVEFAQAGSIAGAADRLFRTPSAITRQLQRLEAELGA
                     ELLDRSTKPPRLNSLGSRVLERARDMLQRTEALRSLASPGAEPHGLLRIGLAHALAEG
                     TLIEPIRALTENYPKVQLRLLSGLTGELFNRLLAGELDVAAVFLPEGKTAPPPLLTNI
                     IAGDHMEIVQGAAGNVGGDWESLGRAPWVLNPPGCLLRASLIDQMERAGVTPMIAAEI
                     HHNVHLHLALIQSGYGVGLLPARFIARKEFIGTVEVLRPPSFELPLSVAIVRVAQLGA
                     LEKAVELLEHGIRHLFEGANTTKPVTRSRRHVPANERKSQSGK"
     gene            1271..3832
                     /locus_tag="VNN25_12255"
     CDS             1271..3832
                     /locus_tag="VNN25_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH92344.1"
                     /translation="MAVAGMVVLGLTATLATAQTHPANYTLDDVMQAPFAWDMVAAPT
                     GTAVAWVFNVKGCSNIWVADPHGAKARQITPYTGDDGFDIGELAWSPDTKSIAFTRGQ
                     SLEDETPSNVNNSPQGPMAREVWVVAAAGGAPHKVGVGDSPNFSPDGSRLLFVEKKRI
                     LTVDASGDGDAQPLLIDQGKVDSLTWAPDGKRLAFVSHRASHSLIGVYDFVDKTIVWL
                     SPSVDYDSSPAFSPDGARIAFIHVPAEKTPDFYSRRSGRPWSIWTADVKTGQARRVWI
                     ADAGPGSVFRPTISTNNDTPSATNLFWTNLDDLVFPWEKSGWQHLYAVPVRGGAARAL
                     TAGKFEVTHVVFSQDRKRLAYSSTQGDPDKMHIWTVDAEHGSAVRAAPQSHAIEDFPQ
                     IGADGALFAVQSDGNQPVHPVALSAGGQWRRLVPEAIPSTFPSSNLVMPQAVTFTAKD
                     GQETHAQLFLPREISSKPHPAILFFHGGPQRQMLLGFDPKRTYSWIYAFNQYLVAEGY
                     VVLSVNYRGGTGYGLDYREAENLGPGGASELNDLLGAITYLRGRGDVDSHRLGIWGGS
                     YGGFMTALGLARASDALAAGVDYSGMHNWSTHLSSQGEPIDGGDANRRAVESSPIATI
                     NQWHSPVLLVQADDDRVVPFQQAAELIEGLRSHNIDHDVIMIPNEIHDMARYSSWMML
                     FNAADGYFDRQLNKRSASDGALDGSAMAQTSEARCALPSEPEGIPAALDAAISGPADK
                     DRACMKGLFIPEARLMLVSLGTDGAPTYRIETLNDWIARVKTRGHVQLEEKQLTFRIE
                     RYGNIAHLWSPYTLQSDGKLVARGTNSIQAIKEAGGWRVTGIMVQAESVTAPPQH"
     gene            3919..4299
                     /locus_tag="VNN25_12260"
     CDS             3919..4299
                     /locus_tag="VNN25_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HXH92345.1"
                     /translation="MSKTAITSPELAPPAGPFSQAIKVDGFLFLSGQVAQDPATGKVV
                     EGGIVPETERVFQNLSAVLKAASKSFDDVVRAGVFLTNMTDFAAMNGIYAKYFSQPFP
                     ARTTIAVAALPLGACVEIDLVVKA"
     gene            4346..4738
                     /locus_tag="VNN25_12265"
     CDS             4346..4738
                     /locus_tag="VNN25_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXH92346.1"
                     /translation="MPLVRIDLLEGKSEQYRAEVGDILYQTLVDVLNVPEHDRFQVIT
                     EHTTANLPFDREYLGIHRSDDCIVFQITLNSGRSTELKQRFYQALADRLHEGVKIRKE
                     DVLINFVEVPKENWSFGNGEAQYVTPAQ"
     gene            4746..5762
                     /locus_tag="VNN25_12270"
     CDS             4746..5762
                     /locus_tag="VNN25_12270"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXH92347.1"
                     /translation="MQMLIVGIGALGGTIAARALRAGLPVRLAARNTESAKVLLRSGL
                     RVSGIGGDVAADTIDVAALEDYGKGEQFDLILLATKAQHALEVAPDVVDLLAPKGVIL
                     PIQNGGVARVLADRLGEDKVLGGFSNLGATMVEPGVYEQKNAGHLMIGELAGGTSERT
                     ERVARVVRRAVEVKISSNMIGAIWSKLLINCSVTTLGALCSQTMRQYMETAAGKEVFR
                     RTYEETLSVALHTGTRLERLAVDPIPPGWSNNSGAGEGYDSWVKAVIDFYGDVKPSML
                     QDLERGRKTEIDFINGYVVAAGNAAGLQVPMNAAITELVHQIERGALQPARERMNELA
                     DQTF"
     gene            5794..6309
                     /locus_tag="VNN25_12275"
     CDS             5794..6309
                     /locus_tag="VNN25_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH92348.1"
                     /translation="MKILTKYGVCALAVALMVPLAQAQPVGIQAAFVKDAGTLSDKFA
                     GLARVMSGKYDWKPGQGVRSVGDVFNLIVVENRMLAGVLSGTPNTGPKPAPITDPEKL
                     QEALKTSYIELQKAITGLSDNDLQSPVKLFGRDMTKQGALMLILADQHEHLGQSIAYA
                     RSNGVVPPWSK"
     gene            6361..6798
                     /locus_tag="VNN25_12280"
     CDS             6361..6798
                     /locus_tag="VNN25_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92349.1"
                     /translation="MTKTIILSMYLAVSALSLNARAQAARQYREGPVTLVQEIAVEYG
                     HFEEYIDWLNSTWKPTMEATKKAGIIIDYKVFSATPQSPDHPSIILWITYQNMAALDR
                     SVEEEEVAKKVICSTECQNKARVGRNEYRKVLRREYIRELILK"
     gene            7814..7936
                     /locus_tag="VNN25_12285"
     CDS             7814..7936
                     /locus_tag="VNN25_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH92350.1"
                     /translation="MSLQVHAGESVGLVGPSGAGKTTVARLALQLLRPARGWVH"
     gene            7936..8463
                     /locus_tag="VNN25_12290"
     CDS             7936..8463
                     /locus_tag="VNN25_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH92351.1"
                     /translation="MGRDLSTLHGRALRDHLRQAHLIFQDPFSALPWHQRVRTIVAEP
                     LRLAGVDRDTRATAVISALAEAGLTPPETYAERNVDTLSGGERQRVALARALIAHPHL
                     IIADEPTSMLDAPVKRAWLERLDALRKEQSLGVLLITHDRAQANAFCDRIIGLEEGFS
                     TLHKAFDDVSSGFGV"
     gene            complement(8634..9296)
                     /locus_tag="VNN25_12295"
     CDS             complement(8634..9296)
                     /locus_tag="VNN25_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92352.1"
                     /translation="MAGNQQVYRLTAVPKEHAEAAYAFHESSGGTFVWPRSLQYIRDL
                     AADGCLFAAWNADGDPVGLAYAEKLEEDVESWEIGGLTVERNARKHGLGTVLLRFALA
                     NVFVYHYKHLPYPFPRIIAHVLASNKRPRHILPELGFEWRGSVEKPRGTVPATFPRDA
                     DGLVRGDVFVYTPRGLIDLSKWFDDFDGTLLGNCRCEFDLGLSSLLLIQGALRALVAE
                     LP"
     gene            complement(9336..9671)
                     /locus_tag="VNN25_12300"
     CDS             complement(9336..9671)
                     /locus_tag="VNN25_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92353.1"
                     /translation="MASTSLRAAAPTTRRSALESLRSKADSFKPEDDEQFRSILAEAQ
                     DLFGMTDGDMATEFLVSRPTVSRWARGKNLPHCLMRRPITEWVFKEANRQLLREQKLQ
                     RSAQLARRA"
     gene            complement(9823..>10429)
                     /locus_tag="VNN25_12305"
     CDS             complement(9823..>10429)
                     /locus_tag="VNN25_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92354.1"
                     /translation="LVVPAMLNRLQDLRDVCPRQELCFVLATNYIDNIEPALVRKGRI
                     DAVIPLVYPDRTSRAALVDARLSRLPAWAERSVRPLLTGNQMNRWPYKAIDTLCGAVI
                     AAASATPAPSHESIVAIVKDLLEDGASFGLSPYDSRRLKNIAESQPFRDEVLAYILSG
                     SSSRKEYLNAIASLSTPTSTNADAMTELTTAGQRMWQRLRG"
BASE COUNT         2184 a         3062 c         3147 g         2036 t
ORIGIN      
        1 tcgagcgtag agtcgatgcg gatgtccaag gctacgttgc gactagcgca ccgctagcga
       61 tcaatcatct tctgcgcact ttcgccggtc gatgaatgaa agtcggtctg aagtacgcat
      121 ttgagatttc ggatgtgggc gggactactt gcccgattgg gacttcctct cattcgcggg
      181 cacatgcctt cggctcctcg ttacgggctt cgtggtgttg gctccctcaa acaaatgccg
      241 aatgccgtgt tccaacagtt cgacagcctt ctccaaagcg ccgagttgtg caacccgcac
      301 gatcgcgacg gacaagggca actcaaatga gggggggcgc aacacttcaa cggtgcctat
      361 gaattctttc ctggcaataa agcgagccgg cagcagcccg actccatatc ctgactgaat
      421 gagtgccagg tgcaaatgta cgttgtggtg gatttcggct gcaatcatgg gggtgacgcc
      481 ggcacgctcc atctgatcga tgaggctcgc tcgaagcaag cacccgggcg gattgagcac
      541 ccaaggtgct cggcccagac tttcccaatc gccgcccacg ttgcctgcgg cgccttgaac
      601 gatctccata tggtcgccag cgatgatatt tgtcaaaaga ggcggaggcg cggttttacc
      661 ttcgggcaag aacacggcag caacgtcgag ctcaccggca agcagccggt tgaacaattc
      721 accggtaagt ccgctcaaca gccgcagttg gaccttgggg tagttttcag tcagggcccg
      781 aataggttcg atcagcgtcc cctcggccag ggcatgcgcg agcccgatgc gcaagaggcc
      841 gtgaggttcg gcgccgggcg acgcaagaga tctcagcgcc tccgtccgct gcaacatatc
      901 tcgcgctcgc tcgagcaccc tggagcccag cgagttcagg cgcggcggct tcgtcgagcg
      961 gtcgagtaac tcagccccca attcagcctc cagcctttgc agttgccggg taatagccga
     1021 cggtgtccgg aatagacgat cagctgcgcc ggcaatggac ccagcctggg caaactctac
     1081 gagcgcgtgt aaatcgtcga gcacaggatc ctcctacgta actccccaaa cctcgtccaa
     1141 acgataccaa gatcgtgcga aggccgcaac atcatcgtga gaataatgaa tttgcggaat
     1201 ggtcggtcct tcgctaccct cgctgagcag aggcaatgga ggtagcgtga acagattagc
     1261 tcgcagagtg gtggccgttg ccggaatggt ggtgctcggg ctgaccgcaa cgctggccac
     1321 cgcacaaacc catccggcga attacaccct cgacgatgtg atgcaggcgc cgtttgcgtg
     1381 ggacatggtg gccgcgccaa cgggcacagc cgtcgcgtgg gtgttcaacg tgaagggctg
     1441 cagcaatatc tgggtggcag accctcacgg cgcgaaagcg cgccagatca caccgtacac
     1501 aggggacgac ggtttcgata tcggggaact cgcctggtcg ccggatacga agtcgatcgc
     1561 cttcacacgc ggccagagtc tcgaggacga gactccgtcc aacgttaata actccccgca
     1621 aggcccaatg gccagggaag tatgggtggt tgccgcagcc ggcggcgcgc cgcataaagt
     1681 cggcgtggga gactccccca acttctcgcc cgatggcagc cggctgctgt ttgtcgaaaa
     1741 gaaaagaatt ctgaccgtgg acgcgagcgg cgatggcgac gcgcagcctt tgctcatcga
     1801 tcagggaaaa gtggactcgc tgacctgggc cccggacgga aagcgcttgg cgttcgtgag
     1861 ccatcgagct agccattcgc tgatcggtgt ctacgatttc gttgacaaaa ccatcgtctg
     1921 gttgagtccg agtgttgact acgacagctc gcccgcgttt tcgccggacg gcgcgcggat
     1981 tgccttcatt catgtacctg ctgagaaaac ccccgatttt tactcccggc gcagcggacg
     2041 gccgtggtcg atctggacgg cggacgtgaa aaccggccag gcgcgacgtg tctggattgc
     2101 cgacgccgga ccgggaagtg tattccgtcc gacgatctcg acgaataacg acactccctc
     2161 ggcgacgaac ctgttctgga ccaaccttga cgatctcgtg ttcccctggg agaaaagcgg
     2221 ctggcagcat ctgtatgccg tccccgtgcg aggcggggcg gcccgcgcac tcacggcggg
     2281 aaagttcgag gttacacatg tcgtcttcag ccaggaccgc aagcgcttgg cctattcctc
     2341 cactcagggt gaccctgaca agatgcatat ctggacggtc gatgccgagc atggttcggc
     2401 ggtgcgcgcc gccccccaga gccacgccat cgaagacttt ccgcagattg gcgccgacgg
     2461 cgctctcttc gcagtgcaga gcgacggcaa ccaaccagtg catccggtcg cgttgagtgc
     2521 gggggggcaa tggcggcggc tcgttccgga agccatcccg tccaccttcc cgagttccaa
     2581 cctcgtcatg ccccaggccg tgacctttac ggcgaaggat ggccaggaaa cgcacgcgca
     2641 actcttcctg ccacgcgaga tcagctccaa gccgcacccg gcgattcttt tcttccacgg
     2701 gggccctcag aggcagatgc tgctggggtt cgatcccaag cgcacgtata gctggatcta
     2761 cgcattcaat cagtacctcg tcgccgaggg ctacgttgtg ctgtcggtga actatcgcgg
     2821 cggtaccggc tatggccttg actacagaga ggccgagaac ctcggccccg gcggcgccag
     2881 cgagctcaat gaccttctcg gcgccatcac ctatctgcga ggccgagggg acgtcgactc
     2941 ccatcgattg ggcatctggg ggggaagcta cgggggattc atgacggccc tcggtctcgc
     3001 gcgcgcctca gatgcactcg cggccggtgt cgactattcg ggcatgcata actggtcgac
     3061 gcacttgtct tcgcagggcg agccaatcga cggaggcgat gcgaaccgcc gggcggtgga
     3121 gtcctccccg atcgcgacca tcaaccagtg gcactcaccc gtgctgcttg tgcaggcgga
     3181 cgacgaccgc gttgtaccct tccagcaagc cgccgaactc atcgaaggct tgcggtcgca
     3241 caacatcgac catgacgtca tcatgattcc gaacgagatt catgacatgg cacgctactc
     3301 gtcgtggatg atgctgttca atgcggcgga tgggtatttc gatcggcaac taaacaagcg
     3361 gagcgcgtcg gacggcgcgc tcgacggaag tgccatggcg cagacgtcgg aggccagatg
     3421 tgcgttgcct tcagagcccg agggcattcc agcggcgctt gacgcggcaa tcagcgggcc
     3481 cgcggacaag gatcgagcct gcatgaaggg cctgttcata cccgaagcgc gcctgatgct
     3541 cgtttcactg ggcaccgacg gagcccctac ctataggatc gagaccttga acgattggat
     3601 cgcccgcgtt aagacgcgtg gccacgtcca gctggaagaa aagcagctga cgtttcgcat
     3661 tgagcgctac ggcaacatcg cgcacttatg gagtccctac acgctgcaaa gcgatggcaa
     3721 gctggtcgcg cgaggcacca acagcataca agcgatcaaa gaagcaggcg gatggcgagt
     3781 tacgggcatc atggtgcagg ccgagtcggt caccgcgcca ccgcagcatt aatgcgcaac
     3841 aggaagcatt atcgtgaaaa taatgaactt gtggaatgtt gcttgtcccg ctacgctgca
     3901 gtagctacag gagatgccat gagcaagacc gcaattacct cgcctgaact cgcgccgccg
     3961 gccggcccat tttcacaggc gatcaaagtc gatggtttcc tcttcctcag cggacaggtc
     4021 gcccaagacc cggccactgg caaggtggtc gaggggggca ttgtgcccga gacggagcgc
     4081 gtcttccaaa acctatcagc cgttctgaag gcggcgagca agagttttga cgatgtcgtg
     4141 cgcgccggtg tgtttctcac gaacatgact gacttcgccg cgatgaatgg catctatgca
     4201 aaatatttca gtcagccctt tcccgcccgc acgacgatcg cggttgccgc actgccactt
     4261 ggggcctgcg tcgagatcga tctcgttgtc aaggcctaag gcgcgcaata gcgttccata
     4321 agccgtcatc atttaaggag gaaacgtgcc tttagtccgc atcgatttac tcgagggaaa
     4381 atcagaacag taccgtgctg aagtcggcga tatcctttac cagaccctcg tggatgttct
     4441 caatgttccg gaacatgacc gctttcaggt aatcacggag cacacgacgg cgaacctgcc
     4501 attcgatcga gagtatctcg gcatacatcg gtcggacgat tgcatcgtct ttcagatcac
     4561 actgaatagc ggccgttcca ctgagttgaa gcaacgcttc taccaagcac tggccgaccg
     4621 tctccacgaa ggagtaaaga ttcgcaagga agatgtcctc atcaacttcg ttgaagttcc
     4681 gaaggaaaat tggtccttcg gtaacggcga ggcccagtac gttacacctg cgcagtagat
     4741 cgacgatgca aatgctgatc gtgggcatcg gcgcgctggg tggaacaatc gccgcgcgcg
     4801 ccctccgcgc cggattgccc gtccggcttg ctgctcgcaa taccgaatcc gcgaaggtgc
     4861 tactcagatc gggcctgcga gtgtccggta tcggaggaga tgtggcggca gacacgatcg
     4921 acgttgccgc cctcgaggac tacgggaagg gagagcaatt tgacctgatc ctgcttgcca
     4981 ccaaggcgca gcacgcgctg gaggtcgcgc ccgacgtggt ggacctgctc gccccgaaag
     5041 gggtcatctt gcctatacag aatggcggag tggccagggt actcgcggat cgtctgggcg
     5101 aagataaggt ccttggtgga ttttcaaatc ttggtgccac gatggtggaa cccggcgtct
     5161 acgagcagaa gaatgcaggg catttaatga tcggcgaact tgctggtggt accagcgagc
     5221 gtaccgaacg ggttgcgcgc gtcgtgcgcc gagcggttga ggtcaagata tcttcaaaca
     5281 tgatcggcgc catctggtcg aagctcctga ttaattgttc ggttacaact ctcggcgcgc
     5341 tttgttcgca aactatgcgt cagtacatgg agaccgcggc aggcaaagag gtcttccggc
     5401 ggacctacga ggagacgttg tctgtggcgc ttcacacagg cactcggctg gaacgtctgg
     5461 ccgtcgaccc gattccgccc ggatggtcga acaattctgg cgcgggggag ggctatgact
     5521 cgtgggtcaa ggccgtgatc gatttctacg gagacgtcaa gccctccatg ctccaggacc
     5581 tcgagcgcgg ccgtaagacc gagatcgatt tcatcaacgg ctacgtggta gcggcaggca
     5641 atgcagccgg actgcaggta cctatgaatg cggcaattac cgaacttgta caccagatcg
     5701 aacgtggagc tctccagccg gcgcgagaac ggatgaacga gctagctgat caaaccttct
     5761 gacgggagta gtgacgaaaa ggagttccag atgatgaaga ttctgacgaa gtacggagtg
     5821 tgtgcgcttg cagtcgctct gatggtgccg ttggcgcagg cgcagccggt aggaatacag
     5881 gctgcctttg taaaagatgc cggaacgctt tccgacaaat tcgccgggct ggcgcgcgtg
     5941 atgtcgggca agtacgactg gaaacccggg cagggcgttc gctccgtcgg cgacgtgttc
     6001 aacctgatcg ttgtggagaa tcgcatgctc gccggcgtgc tgtcaggcac accgaatacg
     6061 gggccaaagc ccgcgcccat caccgatccg gagaaattgc aggaagccct gaagacttct
     6121 tacatcgagc tgcaaaaagc gattacggga ctttccgaca acgacctgca gtcgccggtg
     6181 aaactgttcg gaagagacat gacgaagcag ggcgcgctga tgctgattct cgctgatcaa
     6241 cacgagcatc tggggcaatc gatcgcgtac gcgcgcagca acggtgtggt cccgccgtgg
     6301 tctaagtagg atcagaatat tgctagtcgg gtaggtacat cacaaagggg tgtagagcaa
     6361 atgactaaga ccattatctt atccatgtat ctcgcagttt ccgctctgtc gcttaatgcg
     6421 cgtgcccagg ctgcgcggca gtacagggag ggcccggtca ctctcgttca ggaaatagcg
     6481 gtggagtacg ggcatttcga agagtacatc gattggctga attcaacatg gaagccaacc
     6541 atggaagcca cgaagaaagc tgggataatc atcgattaca aggttttcag cgccactccc
     6601 caatcaccgg atcaccccag tatcattcta tggatcactt atcaaaacat ggcggcttta
     6661 gatagaagtg tcgaggaaga agaagtcgcc aagaaagtaa tctgcagcac cgaatgtcag
     6721 aacaaggcca gagtggggcg gaatgaatac agaaaagtgc tgcgcagaga atatatccgg
     6781 gaacttattt tgaagtgaag tcgtgatccg tcatcgaagg cggcgaggaa tcgcgcgtgc
     6841 tcttcggcat tcagcgacgc tgcgaggtcg gcgcgggaga tgaggttcgt caggtccatg
     6901 caccggccgg aatgcaagga ctccgtcccg cggatcgtcg tcttcaaaac ggcgcgaaac
     6961 gatcttcgtc gcgacgcaaa ttagacgcgc gattagacac ttgggtcgaa aggcgggtgg
     7021 tgggcggatg gggtacggct aggtcgtttt gtggaaatgg gtttcaatct ggtcgggcag
     7081 gggagagtcg gcgcgatgcg cgagctccgc cggaaggact ttgatggcca cgctgcggtc
     7141 gaggcgcgtg tcgcgcgcgc gataaactcg cccatcccgc ccgcaccgag cggcgcaata
     7201 acctcgtaag gaccgaggcg cgttcctggg gtaattggca ccgaggggcc agaatacacc
     7261 acacattcgg cgcgacgacg ttagtaagcg atggaaggaa ttggtggagc tgatcgggat
     7321 cgaaccgatc atctcctcag tgccattttc cgcgtctgtc gagacatacg cggatatctc
     7381 ttttcccgtt ggaggcgcgt ttcgcaccgt ttcgggcggg catcggggat ttgaacggac
     7441 gaccgcctga accccatctc cgcgtctgtc tcgagagcac acgcgggtac gacagcgatc
     7501 gcgcgagaga gatcctcgat ctactcacgc gcctctgccg caagcgaagg aagccgcttc
     7561 tcctgatctc gcacgatctt gcgctgctgg agcgcaactg cgaccgcctg gccattctcg
     7621 accgcggaca aatcgtcgag atcggccctc cttcgcgctc ctcgccgctc ccagcagcgc
     7681 cgccgggcag gcgctcgccg cggccgcgtg cccgcggcca cgcaacccga tccatcgccc
     7741 ggccgatccg acgatcgttc tggacccctc cgcggtcgat ttcacctact ccaccggtgc
     7801 aggtctgaag aacgtgtcgc tgcaagttca cgccggcgag tcagtcggcc tcgtcggccc
     7861 aagcggcgca ggaaagacga cggtggcgcg cctcgccctg cagttgctgc gtccggcgcg
     7921 gggatgggtg cattgatggg gcgcgacttg tccacgttgc atggccgcgc gctgcgcgat
     7981 cacctccgcc aggcccatct catttttcag gatccgttct ccgcactccc ctggcaccag
     8041 cgagtgagaa caatcgtcgc cgaacccctc cgtctcgccg gcgtcgaccg cgacacgcga
     8101 gcgaccgccg tcatctccgc cctcgccgaa gccggcctca ccccgcccga aacgtacgcc
     8161 gaacgcaacg tcgacaccct ctccggcggc gaacgccaac gagtagccct ggcccgagcc
     8221 ctgatcgccc acccccacct gatcatcgcc gacgagccca cttccatgct ggatgccccc
     8281 gtgaaacggg cgtggctcga gcgcctcgac gcactccgca aagagcaaag cctcggcgtc
     8341 ctgctgatta cccacgatcg cgcgcaagcg aatgcattct gcgaccggat tattggattg
     8401 gaagagggct ttagtacgct acacaaggcg tttgacgacg tatcgagcgg tttcggcgtg
     8461 tgacattctg gcgagcagct tggatattga gcttccactc gaagggtcgt gcaaccacag
     8521 cgcaaatttc ttgcccaggg tacttctctc gttctccgcc acgttgagac aaaacgatac
     8581 gtctatcgga gagttttccg cgcgcgcaac agcgcaaatg tcaatcgcca aggctacggc
     8641 agttcggcca caagtgcgcg tagagcgcct tgtatcagca ggagcgaaga caaaccgagg
     8701 tcaaactcgc agcggcagtt gccgaggagc gtcccgtcaa aatcgtcgaa ccacttcgac
     8761 aaatcgatca gccctctggg tgtgtatacg aacacgtcgc cgcgcaccaa accgtctgca
     8821 tctctaggaa aggttgccgg cacagtgccg cgcggttttt cgaccgagcc gcgccattcg
     8881 aagccgagtt cgggcaggat gtgcctagga cgcttattgc tcgcgaggac gtgagcgatg
     8941 atcctgggga atgggtacgg gagatgctta tagtggtaga cgaatacgtt cgcgagagcg
     9001 aaccgtagca acaccgttcc taatccatgc ttgcgtgcat tgcgctcgac agtgaggccg
     9061 ccgatctccc atgactcgac atcctcttcg agcttctccg catatgccag ccccaccggg
     9121 tcaccgtccg cattccacgc tgcgaagagg cacccgtcag cggcgagatc tctaatgtac
     9181 tgaagagagc gtggccagac gaaggtacca cccgacgatt cgtggaaggc gtacgccgcc
     9241 tccgcgtgct ctttcggaac cgccgtcagg cgataaactt gctgattccc tgccatgcgg
     9301 aagggtcagt atacagaaat tatgaggtga tgccgtcagg ctcttcttgc aagctgcgct
     9361 gatcgctgca gtttttgctc gcgcaacaac tgtcggttgg cttctttgaa gacccactct
     9421 gtgataggac gacgcatcag gcaatgtgga aggttcttgc cgcgagccca acggctgacg
     9481 gtcggtctgc tgacgaggaa ctccgtcgcc atatcgccgt ccgtcattcc aaagaggtct
     9541 tgcgcctcag ccaagattga gcgaaattgt tcgtcatctt cgggcttgaa actgtcggct
     9601 ttgctcctca gggattcgag ggcagacctt ctggtagtgg gcgcggcggc gcgtagggat
     9661 gtgcttgcca tgtaactcct cctgatgtgc agtatgatcc atgaaacagg ccaggttcaa
     9721 ccagaaatcg gctttttttg acagcgtctg ctgtgcaaga tgcacaggta gaacgtgaag
     9781 cggtttattt atttgagtgt cgtcgttcac agcctgacag cgctagccgc gcaacctttg
     9841 ccacattctc tggcctgccg tcgtcaactc cgtcatcgcg tcggcgttgg tcgatgtcgg
     9901 cgttgagagg cttgcaattg cgttgaggta ttcctttcgg ctgcttgacc cgctgaggat
     9961 gtacgcgagt acctcgtctc gaaatggctg agattcagca atgttcttga gccgccgcga
    10021 atcgtaaggc gagagtccaa aggaggcacc gtcttcgagc aagtctttga cgatcgcgac
    10081 aatcgattcg tgactcggcg cgggcgttgc agaagcggca gcgatgaccg cgccgcaaag
    10141 cgtatcgata gccttgtacg gccaacgatt catttggttg ccggtgagga gcgggcggac
    10201 gctccgctcg gcccacgccg ggagccttga caggcgggcg tccacgagcg ctgctcggct
    10261 cgtccgatcc gggtagacga gcgggataac ggcgtcgatc cgtcccttcc gaacgagcgc
    10321 cggctcgatg ttgtcgatgt agttcgtcgc gagcacgaaa cagagttcct gccgcggaca
    10381 gacatctcgg agatcctgca gccgattcag catcgccgga accaccagc
//
LOCUS       DATLVW010000410         9181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_387897, whole genome shotgun sequence.
ACCESSION   DATLVW010000410 DATLVW010000000
VERSION     DATLVW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387897"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VNN25_12310"
     CDS             complement(<1..486)
                     /locus_tag="VNN25_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HXH92355.1"
                     /translation="MIRTITATEYVMPLREGGSLPAIVAGDDDGTYVLKFRGAGQGAR
                     ALIAELVSGEIARLLKLPVPEIVFVDLDPRMAKTEPDPEIQDLLRKSEGLNLALDYLP
                     GSITFDPIVALPIGDKSALASSLASSIVWFDALVTNVDRTPKNTNLLVWHRKLWMIDH
                     GA"
     gene            734..1171
                     /gene="rpsF"
                     /locus_tag="VNN25_12315"
     CDS             734..1171
                     /gene="rpsF"
                     /locus_tag="VNN25_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXH92356.1"
                     /translation="MRTYEVLFILSPQVPEEEATTLATEFKRIAEGSGAKLTHEEVWG
                     RRRLAYPIQKFNEGNYQLLVFETEAGLNELDRRMKNSDRILRHMIVRTDLDHKRAEKL
                     AKRNPKKERMRPAPSFAASPGAPVSAPAEPSAMPASDEAVPAE"
     gene            1187..1507
                     /gene="rpsR"
                     /locus_tag="VNN25_12320"
     CDS             1187..1507
                     /gene="rpsR"
                     /locus_tag="VNN25_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXH92357.1"
                     /translation="MATEMRRPGGGGGGGRDSGRGGAGGPGGAQGGKKKFFFRRKRVC
                     KFCVEKLEYIDYKDIKTLQQFIPERGKILPRRISGTCALHQRKLQSAIKRARIAAMLP
                     FATD"
     gene            1520..2554
                     /locus_tag="VNN25_12325"
     CDS             1520..2554
                     /locus_tag="VNN25_12325"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HXH92358.1"
                     /translation="MTETSLQHDEPVATPVRSFGKQARSLAGYTILSALMFVSPLRVF
                     LPAALFHCGIRNGRRAAWLVFVLSAAIAGLLVFGTTRAGNAADAPITFGYLLAICLAI
                     GIPALAALPLVERGEHFGRVLMFALIIGIAGLAITELTMRMVTGASPYAAQLAEAHQT
                     TATMVASYEKAGFPSDAVSFFKKWMDIGTLCLPALLLIDVILVFVFSLVMLGRLRAWR
                     NYALVRDPAVVTAYLFRNLSLPEWLLFAFVASGLTPLASGMVQQIGANVLAVVAFLYL
                     LQGLAIFRSFIARTSAGFIGSLFAWMLLGFLALTGISQILLCMAGLFDSFFDFRHFNR
                     KDHSDESHSH"
     gene            2535..3056
                     /gene="rplI"
                     /locus_tag="VNN25_12330"
     CDS             2535..3056
                     /gene="rplI"
                     /locus_tag="VNN25_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXH92359.1"
                     /translation="MKVILIDEIRGLGSRGDVVTVKGGYARNFLIPKQLAREATPGNL
                     KSIEQERKKWAALGDQEKAAAQKAADSVKGMKIVIHKRVGDHGNLFGSVTSNDIADAL
                     TKKGIDVDKRRIELGHPIKSIGVHDVEVRLHREVAAHIQVEVVPEGKESIEVPPVPAP
                     VAAAPAAAEEAEA"
     gene            3123..4211
                     /locus_tag="VNN25_12335"
     CDS             3123..4211
                     /locus_tag="VNN25_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH92360.1"
                     /translation="MSDDRFVDAPPSGADGWRDTWEHDRRFRPRPSRFDFLLRIFRRF
                     VNRSVEPEEERQRNFNVALLDLLNDIRNDVVRAREDLKRDIETVQRDVRAADESLASE
                     LAKIRELIPIAAKRNDSLIAALDQKIETVAVRVRDVTNPIVAASSATANDFLYRRLED
                     GLRGEVSVSEYVALVRDQQPVIDVGCGRGEFLIACRDAGIVARGFDTNERSVADLVAR
                     GVDASVAGIPGCFVSLTNASVGAVVAMHVVEHLPVDALFALFSESARVLRAGGLLIIE
                     TPNAESLAVSASEFWRDPTHLAPRHPAALTLLGREHGFAIDEIRAIHPFPEGNRLIAA
                     NEETRTLVDALNERIFGGQDLRLVLRRS"
     gene            complement(4322..4993)
                     /locus_tag="VNN25_12340"
     CDS             complement(4322..4993)
                     /locus_tag="VNN25_12340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXH92361.1"
                     /translation="MRRKAPVTMTLLVVIAIVFVFEMLLGGPRFGSNGNVLTGDVNGE
                     VLESLGAIVSGMLVRHEFWRLVTAMFLHASFLHWAVNSWALYQLGTLYEVLFGSKRFA
                     MTYFATGLVASLASAIRIGTTNGLSVGASGAIFGILGAFIFSVARSPVYRHQPWTRGL
                     IGQLVFWIIVNIAIGYSLPFIDNVAHLGGLIAGLILGFIPHRVPPPPPRESVIDVGMM
                     EEPRR"
     gene            complement(4990..5781)
                     /locus_tag="VNN25_12345"
     CDS             complement(4990..5781)
                     /locus_tag="VNN25_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH92362.1"
                     /translation="MGAKLQQKTVVVTGASSGIGRAAALEFARRGANLVLAARRAELL
                     EEIARECRAIGAGCKIVVTDVTQRADCEHLIDMAGSVDVLVNNAGFAIFDAIESARPD
                     DLASMMQTNYFGTVWCTQAVLPQMLARGNGTIVNVASIAGIMGYARMGGYCATKFAVI
                     GFSETLRDEVLRRGVRVAMVCPGTVETEFFVKAERGKMPGASRLIMAIKPEKVARAIA
                     NAAEDGRYRRILPLGAAMFMRFKELCPRLAHTLMRRVSAAMETKS"
     gene            5780..6385
                     /locus_tag="VNN25_12350"
     CDS             5780..6385
                     /locus_tag="VNN25_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXH92363.1"
                     /translation="MTEHSYHVLRFGPMLRRLLLSAVFLTLACHHESDLPKLFPLPEA
                     QLVNDASKPVRMSELKGSVVVYDFIFTHCAGTCPMMTATMRRLTKKIPQDAAVRFVSI
                     SVDPIRDTPAVLHDYAGHVRNDPRWMFLTGDRKTIVDLSVNGFKLAAGDATGSLNESV
                     LHSTKFAVADKNGIIRDYYGGTNDDTVDHVAGVVSELLREN"
     gene            6479..6907
                     /locus_tag="VNN25_12355"
     CDS             6479..6907
                     /locus_tag="VNN25_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HXH92364.1"
                     /translation="MSTILPAVNASLNATTGVLLLIAYVLIRRKKIQMHKRFMLAACT
                     TASIFLVCYVLNHILRHGVVTRFPGTGWVRPLYFTILISHTILAITIVPLVILSVRNG
                     LKMRVPQHRRVAKWTFPLWMYVSVTGVLVYFFLYQWYPQP"
     gene            6945..7553
                     /gene="pdxH"
                     /locus_tag="VNN25_12360"
     CDS             6945..7553
                     /gene="pdxH"
                     /locus_tag="VNN25_12360"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008233686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HXH92365.1"
                     /translation="MTPLLETDLDPDPFEQFDRWFDDAKDAGIAMPEAMTVSTAALDG
                     EVSARVCLLKSFDHRGFVFYTNYCSRKGKQIHDNPRASLVFWWQSLERQVRIEGAVVK
                     TTEEESDEYFATRPRGSQLGAWASEQSRVLAGRGALDARFEELSTTYRDVIIPRPPHW
                     GGYRVIPLLFEFWQGRDDRLHDRFWYRLRNDVKDWVVERLSP"
     gene            7606..8937
                     /locus_tag="VNN25_12365"
     CDS             7606..8937
                     /locus_tag="VNN25_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXH92366.1"
                     /translation="MIVRRLRYLAIAEPIVIVLIFEGLRSITIFSISQRDRLILDGLI
                     IAAFIILGVVMIRAIRRTKQTLERQNAELLALHGAGLDVSAELSLDAVLNKVVERARI
                     LVGARYGALSVVNDDASIQTFLTSGVTRQERARIGPPPSGHGLLGVVLHEGERLRLPD
                     IGKDPRSQGFPPNHPVMRSLLAVPVTCKGPFAGNLYLSEKEDGGEFTPGDEETLERFA
                     VQAAIAIDNAHMHRQVADLAVAQERLHIAREIHDGTAQVLGYVNTKAQAAREYIRQNK
                     MEEGMEQLRELAAAAREAYSDVRESIVDLRTLSGPSRSLHVVLEEYIAHWKMQAGITT
                     DLLLDDDITVPPGMELQVVRIIQESLANVRKHAKATKASLEVRKRHGKLSVIVRDDGV
                     GLPEAPRKTDFPRFGISTMRERAESIGGTFSIESNPGGGTVVHAAIPLPGN"
     gene            8957..>9181
                     /locus_tag="VNN25_12370"
     CDS             8957..>9181
                     /locus_tag="VNN25_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH92367.1"
                     /translation="MRILIADDHPLFRDGLRSLLTAQGHEVVGEAHNGHEAVALAQKL
                     RPELVLMDVSMPELDGIGATRQITADVPDVK"
BASE COUNT         1695 a         2900 c         2842 g         1744 t
ORIGIN      
        1 cgcgccgtga tcgatcatcc agagtttgcg atgccagacg agcaggttcg tgttcttcgg
       61 cgtacggtca acgttcgtga cgagcgcatc gaaccagacg atcgatgacg ccagcgatga
      121 tgcaagcgcc gacttgtccc cgattggcag cgccacgatc ggatcgaatg tgatcgagcc
      181 gggaagatag tcgagcgcga gattgagccc ctcgctcttg cgcagcagat cctgaatctc
      241 cggatcgggc tccgtcttgg ccatccgagg atcgagatcg acgaagacga tctccggcac
      301 aggaagcttc aacagccgcg cgatctcgcc cgagacgagt tccgcgatca gtgcccgcgc
      361 gccctgaccc gcgccgcgaa acttcagcac gtacgtgccg tcgtcatcgc ccgcaacgat
      421 cgccggaagc gagcccccct cgcgcaaagg catcacgtac tcagttgctg tgatcgtcct
      481 gatcattagt ggatcataaa ggagtgcagg cgtctcgcct gcggccggcg ggcgtcctcg
      541 cccgacggaa ttcttccggg gatgagcgga ccgaagttga gaaagatcct tttgaaataa
      601 attgctcctg ctcaaccctt tgcgtatcat ccccggcccg gctctccgcg gtcgggacat
      661 cttttgacaa tctgtccagc tccttgagcc atccgctcca ggcggaggct ccgctaggcc
      721 agaaggagga tttatgagga cttatgaagt cctgttcatc ctttctccgc aagtaccgga
      781 ggaagaggcg acgacgcttg ccactgagtt caagcgtatc gccgaggggt cgggcgcaaa
      841 actcacccac gaagaagtgt gggggcggcg ccggctcgca tatcccatcc agaagttcaa
      901 cgaaggcaac taccagctcc tcgtgttcga gaccgaggcc gggctcaatg agctcgaccg
      961 ccggatgaaa aactcggacc ggatcctgcg ccacatgatc gtgcgcacgg atctcgacca
     1021 caagcgcgcc gagaagcttg ccaagcgcaa cccgaagaag gagcgcatgc gtcctgcccc
     1081 ttcgttcgca gccagcccgg gagcccctgt ttcggctccg gcggaaccct cggccatgcc
     1141 cgcttccgac gaagcggtgc cggctgaata aggagaggag actgatatgg caaccgaaat
     1201 gagacgacct ggtggtggcg gcggtggtgg ccgcgattcc ggccgcggcg gcgccggcgg
     1261 acctggcggt gcgcagggtg gcaagaagaa gttcttcttc cgccgcaagc gcgtctgcaa
     1321 attctgcgtc gagaagctcg agtacatcga ctacaaagac atcaagacgc tgcagcagtt
     1381 cattcctgag cgcggcaaga ttcttccgcg ccgcatctct ggaacgtgcg cactgcatca
     1441 gcgcaagctc cagagcgcaa tcaaacgtgc gcgcatcgca gccatgctgc cgttcgcgac
     1501 cgactaagca accattctga tgaccgagac gtctctgcag cacgatgagc cggtagcgac
     1561 accggtgcgt tcgttcggga agcaggcccg cagtctggcc ggctacacga ttcttagcgc
     1621 gctgatgttc gtgtcgccgc tgcgcgtctt cctgcccgcg gcgctgttcc attgcggtat
     1681 ccgcaacggg cggcgcgccg cgtggctggt cttcgtgtta tcggcggcga tcgccgggct
     1741 gctcgtgttc gggacgaccc gcgcgggaaa cgctgccgat gcgcccatca ccttcggcta
     1801 tctgctcgca atctgtctcg ccatcggcat cccggctctg gccgcgcttc cgttggtcga
     1861 gcgtggtgag catttcgggc gcgtgctgat gttcgcgttg atcatcggca ttgccggtct
     1921 cgcaatcacg gagctgacga tgcggatggt gaccggtgcg tcgccgtacg ccgcgcagct
     1981 cgcggaagcg catcaaacaa ccgcaacgat ggtggccagc tacgagaaag cagggtttcc
     2041 ttccgatgcc gtcagtttct tcaagaaatg gatggacatc ggcacgctct gcctgccggc
     2101 gctgctgctg atcgacgtca tcctcgtatt cgtattcagc cttgtcatgc tcggccgtct
     2161 tcgcgcctgg cgcaattacg cgctcgttcg ggatccggcc gtggtcactg cgtatctgtt
     2221 ccggaacctc tcgttgcccg agtggctgct cttcgcgttc gtggcaagcg gtctcacgcc
     2281 gctggccagc gggatggtgc agcagatcgg cgcgaacgtg ctggcggtgg tcgcatttct
     2341 ctacctgttg cagggattgg cgatcttccg gtcgttcatc gcccgtacca gcgcgggatt
     2401 catcggctcc ctcttcgcct ggatgctcct cggcttcctg gcgttgaccg gcatctccca
     2461 gattctgctc tgcatggcag ggctatttga ctcgtttttc gatttccgac atttcaatcg
     2521 aaaggatcac tctgatgaaa gtcattctca ttgacgagat ccgcgggctt ggaagccgcg
     2581 gtgacgtcgt cacggtcaag ggtggctacg cgcgcaattt cctgattccc aagcagctcg
     2641 cgcgcgaagc aacacccggt aacctgaagt cgatcgaaca ggagcgcaag aagtgggctg
     2701 cgctcggcga tcaggaaaaa gcggcagcgc agaaagcggc cgacagcgtg aaggggatga
     2761 agatcgtcat ccacaaacgc gttggcgatc acggcaacct cttcggctcg gtgacgtcga
     2821 acgacatcgc cgatgcactg acgaagaagg gcatcgacgt cgacaagcgc cgcatcgagc
     2881 tcggacatcc gatcaagagc atcggcgtgc acgacgtcga agtgcgcctg caccgcgaag
     2941 tggccgccca catccaggtc gaagtcgtcc ccgaggggaa ggaatcgatc gaggtgcctc
     3001 cggttcctgc gccggttgcg gctgctccgg ctgcggcaga agaagcggaa gcgtaggttt
     3061 ttcgcgaggg tgcacagaca ctcctgtgca ccctcaagtt tcctgctgat atcctccctt
     3121 caatgagcga cgaccgtttc gtcgatgctc cgccgtcggg agccgatggc tggcgcgaca
     3181 cgtgggagca cgaccggcga ttccggccgc ggccgtcgcg tttcgatttt ctactccgca
     3241 ttttccggcg tttcgtcaac cgttccgtcg agccggagga ggagcggcag cgcaacttca
     3301 acgtcgcgtt gctcgatctg ctcaacgaca tccgcaacga cgtcgtgcgg gcacgcgaag
     3361 atctcaagcg cgacattgaa acggtgcagc gggatgtgcg cgccgccgac gaatcgctgg
     3421 ccagcgagct ggcgaagatt cgcgagctga ttccgatcgc ggcgaaacgc aacgattccc
     3481 tcatcgccgc gctcgatcag aagatcgaga ccgtcgccgt tcgcgtgcgc gacgtgacga
     3541 acccgatcgt cgcggcttcc agtgcgactg ctaacgactt cctctaccgc cgcctcgaag
     3601 acggtcttcg gggcgaggtg tcggtgagcg agtacgtcgc gctggtgcgt gaccaacagc
     3661 cggtcatcga tgttggctgc ggccgcggcg agtttctcat cgcctgccgc gacgccggca
     3721 tcgtcgcgcg tggcttcgac acgaatgagc gctcggttgc ggatctcgtc gcacgtggtg
     3781 ttgatgcgtc cgttgccgga ataccggggt gtttcgtttc gctcacgaat gcctcggttg
     3841 gcgccgtcgt ggcaatgcac gtcgttgagc acctgccggt cgatgcgctc ttcgcgctgt
     3901 tttccgaatc ggcgcgggtg ctgcgtgccg gtggcttgct catcatcgag acgccgaacg
     3961 cggagtcgct ggcagtcagc gcgtccgagt tctggcgcga tccgacacat ctcgcgccgc
     4021 gccatcctgc cgcgctgacg ctgctggggc gcgagcatgg atttgcgatc gatgagattc
     4081 gcgcgattca cccgttcccc gagggcaacc ggttgatcgc cgcgaacgaa gaaacgcgga
     4141 cgctcgtcga tgcgctgaac gagcggatct tcggcgggca ggatctgcgg ctggttctgc
     4201 gtcggagctg attgaatgga gcacagacaa gagtgtctgt gccacaccat gcttcgctta
     4261 ataagcagta gtaggtagag atccgcgggt ggtgtggcac agacactctt gtctgtgctt
     4321 gttaccttcg cggctcttcc atcatcccca catcgatcac gctctcccgc ggtggtggcg
     4381 gcggaacacg atgcgggata aatccgagaa tcagccctgc gatcagtccg ccgaggtgcg
     4441 cgacgttatc gatgaacggc agcgaatagc cgatagcgat gttgacgatg atccagaaga
     4501 cgagctgtcc gatgaggcca cgcgtccagg gctgatgccg gtaaacaggc gatcgagcga
     4561 ccgagaagat gaacgcgccg aggatgccga agattgcgcc gctggctccg accgaaaggc
     4621 cgttcgtcgt gccgatgcga atggcgctag cgagcgaggc gaccaggcca gtcgcgaagt
     4681 acgtcatcgc gaaacgcttc gaaccgaaca acacttcgta gagcgttccg agctgataga
     4741 gagcccacga gttgaccgcc caatgaagaa agctcgcgtg caggaacatc gcggtgacga
     4801 gccgccagaa ttcgtgacgg acgagcatcc cgctgacgat cgcgccgagc gattccagca
     4861 cctcaccgtt gacgtcgcct gtcagaacgt tgccattcga tccgaaacgc ggaccgccga
     4921 ggagcatctc gaagacgaag acgatggcga tgacaaccag caacgtcatc gtcacgggag
     4981 ctttgcgtct catgacttcg tctccatggc cgccgaaacg cggcgcatga gagtgtgggc
     5041 caggcgcggg cagagctctt tgaatcgcat gaacattgct gcgcccaacg gcaggatgcg
     5101 ccggtacctt ccatcctcgg ctgcattggc gatggcgcgg gcgactttct ccggcttgat
     5161 ggccatgatc agccgcgacg cgcccggcat cttgccgcgc tccgctttca cgaagaattc
     5221 ggtctcgacg gttcccggac agaccatggc cacgcgcacg ccgcggcgaa gcacttcatc
     5281 gcgcagcgtc tccgagaacc cgatcaccgc aaacttcgtt gcacaatagc cgcccatccg
     5341 cgcgtagccc atgatgccgg cgatcgaggc tacgttgacg atcgtcccgt ttccgcgcgc
     5401 cagcatttgc ggcagcactg cctgcgtgca ccagacggtg ccgaagtagt tcgtctgcat
     5461 catcgacgcg agatcgtcgg gacgggcgga ttcgatggcg tcgaagatgg caaagccggc
     5521 gttgttgacg agcacgtcga cgctgccagc catgtcgatg aggtgctcgc agtcggcgcg
     5581 ctgcgtgacg tcggttacga cgattttgca gccggcgccg atcgcgcggc actcgcgggc
     5641 gatctcttcc agcagctccg cgcgccgcgc ggccaatacc aggttggctc cgcggcgcgc
     5701 aaactccagc gccgccgcgc ggccgatgcc gctcgatgcg ccggtgacga cgacggtttt
     5761 ctgttgcagt ttcgcgccca tgacagagca ctcatatcac gtgctacgct tcgggccgat
     5821 gctcaggcgc ctccttctct cagccgtctt tctcactctg gcctgccacc acgaatccga
     5881 tctcccgaag ctctttcccc tcccggaagc gcagctcgtc aacgacgcaa gcaagccggt
     5941 gcggatgagc gagttgaagg gcagcgtcgt cgtctacgac ttcatcttca cgcactgcgc
     6001 gggcacgtgc ccgatgatga cggcgacgat gcggcgactc acgaagaaga ttccgcaaga
     6061 cgcagcggtt cgcttcgtct cgatcagcgt cgacccgatc cgcgatacgc ccgcggtgct
     6121 gcacgactac gccggccacg tacgcaacga tccgcgctgg atgttcctca ccggcgaccg
     6181 caagacgatc gtcgatctct ccgtgaacgg attcaagctc gccgcgggcg atgccaccgg
     6241 cagcctgaac gagtcggtgc tgcacagcac gaagttcgcg gtcgccgaca agaacggcat
     6301 catccgcgac tactacggcg ggacgaacga cgatacggtc gatcatgtgg ccggcgtagt
     6361 gagtgagctg cttcgcgaaa actgatgatg aaaggatgaa ggatgaagga tgaagaacag
     6421 attgcgttca tccttcatcc ctcatccttc atccttcgag atgctacagt cgcgcgccgt
     6481 gtccacgatc cttcccgcgg tcaacgcttc gctcaacgcc actaccggcg tgctgctcct
     6541 catcgcatac gttctcatcc gccgcaagaa gatccagatg cacaagcgct tcatgctggc
     6601 agcgtgcacg acggcgtcga tctttctggt gtgctacgtc ctcaaccaca tccttcggca
     6661 cggcgtggtc acgcgctttc cgggcaccgg ctgggtgcgg ccgctctatt tcacgattct
     6721 catctcgcac acgattctcg ccatcacgat cgtgccgctg gtgatcctgt ccgtgcggaa
     6781 cgggctgaaa atgcgcgtgc cgcagcatcg gcgcgtggcg aaatggacgt ttccgctctg
     6841 gatgtacgta tcagtgaccg gcgtgctggt gtattttttt ctctatcagt ggtacccgca
     6901 gccgtgagcg tgcgatatct tggcggtagg agttctctgt gagtgtgacg ccgcttctcg
     6961 agaccgacct cgaccccgac ccgttcgaac agttcgaccg ctggtttgac gacgcgaaag
     7021 acgccggcat tgccatgccc gaggcgatga cggtgtcgac ggcggcgctc gatggtgaag
     7081 tctcggcgcg cgtctgcctt ctcaagtcct tcgatcaccg cggcttcgtt ttctacacga
     7141 actactgcag ccgcaaagga aaacagattc acgacaatcc tcgggcctcg ctcgtcttct
     7201 ggtggcaatc gctcgagcgg caggtgagga tcgaaggcgc cgtcgtcaag acgaccgagg
     7261 aagagtcgga cgagtacttt gcaacgcgtc cgcgcggcag ccagctcgga gcctgggctt
     7321 ccgaacagag ccgcgttctg gctggccgcg gcgcgctcga cgcccgattc gaagagttgt
     7381 ccacgaccta ccgcgacgtc atcattccgc gcccccctca ctggggcggc taccgcgtga
     7441 tcccgcttct gttcgagttc tggcagggac gcgacgatcg tctgcacgat cgtttctggt
     7501 accgcttgcg caacgatgtg aaggattggg tggtggagcg gttgtcgccg taagcaccgc
     7561 gcgtcctctc gacgcgcaac tcgttccgaa ataccctacc ttccaatgat cgtacggcgc
     7621 ttgcgctacc tggccatcgc ggagccgatt gtcatcgtgc tcatcttcga ggggctgcgg
     7681 tcgatcacga tcttctcgat ctcgcagcgc gaccggctga tcctcgatgg cctcatcatc
     7741 gccgcgttca tcatcctggg cgtcgtgatg attcgtgcga tccgccgcac caagcagacg
     7801 ctcgagcgcc agaacgcgga gctgctcgcg ctgcacggag ccggtctcga cgtcagcgcg
     7861 gagttgtcgc tcgacgccgt cctgaacaaa gtcgtcgagc gggctcggat cctggtcggc
     7921 gcacgatacg gagcactctc cgtcgtcaac gatgatgcct cgatccagac ctttctcacg
     7981 tcgggtgtga cgaggcagga gcgcgcccgt atcgggccgc cgccttccgg acacggactc
     8041 ctcggcgtcg ttcttcacga aggggagcgt ctgcggctgc ctgacatcgg caaggacccc
     8101 cgctcgcagg gattcccgcc caaccacccg gtgatgcgtt cgcttctggc cgttccggtc
     8161 acgtgcaaag gtccgtttgc cggcaacctc tacctcagcg aaaaagagga cggcggcgag
     8221 ttcacgcccg gcgatgagga gacgctggaa cgcttcgccg tgcaggccgc catcgccatc
     8281 gacaacgcgc acatgcaccg ccaggttgcg gatctcgcgg tggcgcagga acggttgcac
     8341 atcgcgcgcg agatccatga cggcaccgct caggtcctcg gctacgtgaa cacgaaggct
     8401 caggcggcga gggagtacat ccgccagaac aagatggaag aggggatgga gcagttgcgg
     8461 gagctggctg cggcagcgcg cgaagcgtac agcgacgtcc gcgagtcgat cgtcgatctc
     8521 cgcacgttat cgggcccctc gcggtcgctg cacgtcgtgc tcgaggagta catcgctcat
     8581 tggaagatgc aggctgggat cacgaccgat ctattgctcg acgacgacat taccgtgcct
     8641 cccggtatgg agctgcaggt cgttcgcatc atccaggaat cgctcgccaa cgtccgtaag
     8701 cacgccaaag cgacgaaggc gagtctcgag gtccggaagc gccatgggaa gctgtcggtc
     8761 atcgtgcgcg acgacggcgt cggcctcccg gaagcgccgc gcaagaccga tttcccgcga
     8821 ttcggcatct cgacgatgcg cgagcgggcc gaatcgatcg gcggcacctt ctcgatcgaa
     8881 agcaatcccg gcggcggcac cgtcgttcat gccgcgattc cgctgcccgg caactgacgg
     8941 ttacactgat caacgcgtgc gcatactcat cgctgacgat cacccgctgt ttcgcgacgg
     9001 ccttcgcagt ctcctgaccg cccagggcca cgaggtcgtg ggcgaggctc acaacggcca
     9061 cgaagcagtc gcgcttgcgc aaaagctgcg gcccgagctc gtgctgatgg acgtttcgat
     9121 gccggagctc gacggcatcg gcgcgacgcg gcagattacg gcggacgtgc ccgacgtgaa
     9181 g
//
LOCUS       DATLVW010000411        14688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_16791, whole genome shotgun sequence.
ACCESSION   DATLVW010000411 DATLVW010000000
VERSION     DATLVW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16791"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..527)
                     /locus_tag="VNN25_12375"
     CDS             complement(180..527)
                     /locus_tag="VNN25_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH92368.1"
                     /translation="MSENSAQLVAEVVVPDLGKTIDFLTALGFRVERIDGTFAVLRWD
                     DSYLFVAEDAGAKTTARWINIRVIVPDVDAMWSKATQLGRIVTMIGDRSYGLRDFIVA
                     GPFGVEIRFAQII"
     gene            complement(531..1370)
                     /locus_tag="VNN25_12380"
     CDS             complement(531..1370)
                     /locus_tag="VNN25_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92369.1"
                     /translation="MNRLRTLPALVLLLLAGNAAADDAHGTFQFGKAKFQIVNAMAWQ
                     REGKDPKKPVTVVAGADFKIDQPAVIAALDPANALIAQIVKAERGNFVIITLAPADHC
                     AIFAFLDTGKQIDLGDSFPAKTTTSTAAHITGDCSTAKPEKTLFGDPYAFHLAYDLPI
                     TPMPKPSALPAGGGEPGQAFVALIGAIRANDWDATHNHLREEEVPKTAPKPSERKEYF
                     EGLALNYPKTVTVTGGQIKGDQAQVQITGTKYDGGKIKGDIAMKKVAGNWRVVDMTLY
                     GAD"
     gene            1501..3900
                     /locus_tag="VNN25_12385"
     CDS             1501..3900
                     /locus_tag="VNN25_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HXH92370.1"
                     /translation="MRFEHVRAALVLVAAFVLPELFTVAAQSVDVITPTSAPHLRASD
                     ARTPHVETAPLPVQNFTVSRGIGILEDDGKSLYPGFPSPIHGQETWRATPTANNTFIL
                     KKVAYQFDDPSAGTVIYDGAKRPIVGFLDANYIPPHYTLPAPFTFAGKQYTKLSISTW
                     GAIAFGDADSTQVNYDPTVVSNMFRVPMVAVWYELFYYPSNARVIARNKANSVVITWQ
                     NVLSRHSPTPCTFQAELFFDSGEIQLSYQTLPVPDGLVGFSTGTEAVTHQATAAVSNP
                     DIPAYLQAASASFDNYGGIIAGVTLKVNAPIPVPAAGESFRYTLLINGLEVIALEYTS
                     SQSILVVVPVPKIPEFPGAQIQSWETKLSGDTLSVRMPATSLVPYLSTTGTSTWAIKS
                     TRFGGAGFLERTITQTLPLNFTVRQSLLPTQIDAAGVEVPAEIFHYTPDVWDTDRIRE
                     SIAAYLVSRGQSVDSFRFFPTSYDDGLKHSNYAGTYPRQKSVTGAGQIPARTECDCHY
                     GAEFSSVSESLGTESTTQIALTHELGHECVFYADYKDVDGVVKGPWRDAGVPCFGGAH
                     PNNGLANESMFADSESGLKTISVMGGSVGGVYQLTAPRFGFSRVEMYFLGLASPAEVT
                     PITFVQSSGTSQITIDQVVAANGVRTPSYGGQQKVFRVPAFVVKRQEETVSDAQLQQL
                     QNLLFRWQSRFWRETGGRARSNTTLDGGCSYTLSASSVKIVSDGSTGSVNVFVEPGCT
                     VTASTTDPWISVNVNGAGAASYVVGPNASGNARNGTITIAGQPFTVNQYGKGRREAAG
                     H"
     gene            complement(3962..4180)
                     /locus_tag="VNN25_12390"
     CDS             complement(3962..4180)
                     /locus_tag="VNN25_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92371.1"
                     /translation="MSQKTSELEEDDIPEMDLHGGARGKYYERYMQGTNVILLDPDLA
                     DVFHDSAVVNQALREYLSEHGAPPERTR"
     gene            complement(4164..4439)
                     /locus_tag="VNN25_12395"
     CDS             complement(4164..4439)
                     /locus_tag="VNN25_12395"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXH92372.1"
                     /translation="MKFEWDHKKNLSNLSKHRLSFAEASTVFGDPLHRTLADPRHSFE
                     EFRFLTTGYTSAGRLVIVAHSQRGERVRIITARLALPKERRFYESEN"
     gene            4523..6238
                     /gene="recJ"
                     /locus_tag="VNN25_12400"
     CDS             4523..6238
                     /gene="recJ"
                     /locus_tag="VNN25_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXH92373.1"
                     /translation="MLHDAWHMLEAPRELPDLPIGNDKLRAILVRRGITTLDDYRRFV
                     APCIDDLHDPSTIHGIGDACARIERAVRDRETILIYGDYDVDGVTSIVLLQTVLRALG
                     ADAAHVVPHRLVDGYGLKTVVLDRVLAERDVRLVITVDCGITSVDPVRVAIERGIDVI
                     ITDHHLPPEMLPAAAAVLNPKQEGCEYPFKDLAGVGVAFKLCCELLRRSGSKMSIASL
                     LKIAAIGTVADVAPLIGENRTIASLGLAGLADPRNPGLRALIRRLGMLGRPLRAVDIG
                     FKIGPRINAAGRLSSAETAIDLFAAASEESAWTLCAELDRLNAERQEIEKGVRAEAEE
                     EVARMGSEHIFVLAGHNWHKGVLGLTAGRIAQRYHRPTLAIGIEGDVAVGSGRSIGTI
                     NLHEQLESVAELFTHFGGHEFACGFSLPASNVDELRRRLEERFASLDDALFLRESQVD
                     AEVTLAEIDREFVAAHEMLQPFGAGNLQPLFLARNASVIATREFAEDCCEITIDDGTG
                     RGLAVLWPSACALAPDLLRGATVDLLFQIEPDSYSPTGAKLVIVDARSASVSLAASGA
                     SRPGG"
     gene            6375..8363
                     /locus_tag="VNN25_12405"
     CDS             6375..8363
                     /locus_tag="VNN25_12405"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HXH92374.1"
                     /translation="MQRTIRILRVALPLVFIGFVLVIALSWTRGKPKRDKAGTIPVTS
                     TIRPIDKPAIVSTTFEDTQTINGRLASHIRAERVVAFQSGWNTLENVQMTIYRPKGLT
                     YEVVCPQAQFNSNTKESDAKGGVRVTSSDGVEITTAEIHFDGVHLTNHVAVNFKIDRW
                     TGSGGALDMDVPGEMVRLYEKVDATMAPQTPAESPLNIKGQEGVFRRKENYADFTKDV
                     VATRDSDRFTVDHVVGRFGADHKALMALEGQGHLTIVMTGDSALAGKSSETEGRKTIT
                     ADRFFSEIGATGEISAMNFVGDTAPAHAVIEGPPNRDLVAHTFRVGIANKQVTEMKAD
                     DNVTMRETAPIKRLMTSGHLVVYFDPATHRAASAALDGNVHYQDPKNDAHSVRANYDI
                     TGDHVTLTGEPGFFPSVVSDGQTLKAKLIEFSPRAGTARGTGEVIALLVSKQANGAPA
                     AVSADATSIFPAGKPVFVNSDTVMMQQATKVGIFSGHVRAWQETNTVFSQELQVQGAG
                     DQITARGNVRTLLYNTSTKAAAEVRKVPMQSTSDVLIARKADRRIDLNGSVQIDDGEG
                     RHFSSEHASFFFDAAKKMDHIDAQEKVVLVEKATMRRGTGDKATYNVAQRMVYLIGSP
                     ALVTTPTGSLPGKKISLDLERNKVDVVEPMQGTYKPPP"
     gene            8578..9300
                     /gene="lptB"
                     /locus_tag="VNN25_12410"
     CDS             8578..9300
                     /gene="lptB"
                     /locus_tag="VNN25_12410"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXH92375.1"
                     /translation="MRLAAEHLVKVYKRRQVVKDVSVEIKQGEIVGLLGPNGAGKTTT
                     FYMIVGLVRPDDGKVTLGSDDITALPMYLRAQRGISYLPQEASVFRKLTVEENLLAVF
                     ETMDLPLAERKRRTRMLLEEFGITHIAKNRAFSLSGGERRRVEIARSLAINPAFILLD
                     EPFAGIDPIAVFEIQRIVSQLRARGIGILITDHNVRETLKITDRAYIIKEGEIFRQGV
                     PESLSADAEVRRIYLGESFNLN"
     gene            9343..10794
                     /gene="rpoN"
                     /locus_tag="VNN25_12415"
     CDS             9343..10794
                     /gene="rpoN"
                     /locus_tag="VNN25_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXH92376.1"
                     /translation="MALEQKLHLKLSQKLIMTPSLQQAIKLLQLSKLELQEVLNQELL
                     ENPLLEESADEAKQEEAEVEAQEKTQTEEEAKAAEPAPEKEKDSFDEIDYDAYFQDYI
                     EYGYNPRMGEDHEEFPIENTLTRPPNLTDHLAWQLGMSDASPAVKDIGAFIIGNIDED
                     GYLRATSEEIAAAGPYDPADVEKAIAAIQSLDPIGVGARDLRECLLLQLQFLEVDNTL
                     VESIVRDHWDLFMQRHFVQIAKALGIDMKTLEGVVEIIKHLDPKPGRKYSNERAIYVE
                     PDVYIQKVGDDYVIVLNEDGMPKLRINGSYRSMLNSMDSKSDGETVNYIKDKIRSAVW
                     LIKSLDQRQRTIYKVAESIVKHQREFLEKGIDYLRPLVLRDVADDIQMHESTVSRVVS
                     NKYMHTPRGLFLMKYFFHSGIDSDTGEDISSLTVKKKIQGFIDGEDPRKPLSDSKIMK
                     ILNDEGINIARRTVAKYRDELNIPSSTDRKQIF"
     gene            10829..11179
                     /gene="raiA"
                     /locus_tag="VNN25_12420"
     CDS             10829..11179
                     /gene="raiA"
                     /locus_tag="VNN25_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXH92377.1"
                     /translation="MAAVTPEIVGRNFEVTPDIRALIESKLGRVEDRLFQDVSSVRVM
                     LHVEKFRYTCEILVVAKDHDAKAVQESETSMADAINAAVDHIKRQAQKSREKIRDHHR
                     GGPEPVPEGNSRVS"
     gene            11213..11791
                     /gene="raiA"
                     /locus_tag="VNN25_12425"
     CDS             11213..11791
                     /gene="raiA"
                     /locus_tag="VNN25_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXH92378.1"
                     /translation="MATTIAGRHYTVTEDIRTLITTKVGKIVEKLFNDVIDVRVVLQV
                     EKYRNICEILIKGKDHDVKSVQESGESMQDAINKALDHVKRQAQKNRKTIRDHHRKDG
                     VLNKSVAGQVTEWAVQVLEPGKLRKGGKARLPRIIKTNSLAILPMTIEDAAESLDGSK
                     NEFIVFRDLDNDRISVIYKRRDNNLGLIAPEV"
     gene            complement(11876..12550)
                     /locus_tag="VNN25_12430"
     CDS             complement(11876..12550)
                     /locus_tag="VNN25_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXH92379.1"
                     /translation="MRKRHLIGAAVLCFAAVAAFAGGLSKYKGWDKTPQGYLMTASER
                     SQWASVKTDDEAEKFVKDFLARRGPDFADQVNFAAAQADKYFTTGKTPGSRTERGRLV
                     IILGPPSAMSKTDTPVRGALRTPSGGALTNMNEGGGSANSAGGSVDDYAGAANNAGAG
                     DSMLHTYSFTYDAKRLPPRFDSNLDVVIAVDPGGRERIDRKTQADLDKVYEMAAQLKL
                     IQPASR"
     gene            12755..13435
                     /locus_tag="VNN25_12435"
     CDS             12755..13435
                     /locus_tag="VNN25_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXH92380.1"
                     /translation="MKTRLLFAAGILLFSASLLADGLVKFKGWDSSPQGYFMTRAERQ
                     QWASVRTDDEAQRFVDTFLASRGPGFAAEVASRVEQADKHLTIEGTHLPGSKTLRGKL
                     IILLGAPTSYDASDFTDRSSVHQDSPIMANALSGGSSGDSGGGGKGGADLGTGGSDMS
                     EGARTMGAGRTIRSFHFGFASTPAGPMEVTINADTNTGKDRPRGKDDSKRLDAAFEAA
                     AQASIKTK"
     gene            13520..14602
                     /gene="hprK"
                     /locus_tag="VNN25_12440"
     CDS             13520..14602
                     /gene="hprK"
                     /locus_tag="VNN25_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXH92381.1"
                     /translation="MTNQAAEDFIPTTVGVLRSGDLDDLSLRLVTGEDGLDRVILRPR
                     VQKPGLAFAGYYEYIKQWRVQIIGESEIKYLLSLPPRLREKRVRDVAALDVSCFIVTK
                     GIAPLEEFRHECEKRSVPLFSTQAMSSTSITRTTYFLEETMAPKLTMHSGLLDVYGIG
                     VLLLGDSGIGKSECALDLIYRGHRLIADDMVVIKRHPNDVLLGYSNDLLRHHMELRGI
                     GIIDIKDLFGVASTRDVKPIDLVVRLEKWVEGTEYDRLGITGETFELLGVSKPYVRLP
                     VASGRNLALLVEIAARNHLMKLQGHDSARSFLQRIDEQIAKQGSEEGSVLPSSNPEEK
                     ASSELRSMSTKMRFQADVIERTRRQR"
BASE COUNT         2945 a         4443 c         4561 g         2739 t
ORIGIN      
        1 catgaactgg aacgatctcg acgccggcaa ggcggcgcct gacgtcggcg gcccacttgc
       61 ggttgttgtc gaagaggtgg ctgaggaagc gcatggtttc gggggaggat agcgtagtgc
      121 tttagttggg agaaccgttt cacgcggagg cgcgggggac acggaggtaa atgctttagc
      181 taaatgatct gcgcaaagcg aatttctacg ccaaagggac cagccacgat gaagtctcgc
      241 aatccgtagc tacgatcgcc gatcatcgtg acgatgcgtc ccaattgcgt ggccttcgac
      301 cacatcgcat cgacgtccgg cacgatcacg cggatgttga tccatcgggc cgtggtcttg
      361 gctccggcat cttccgcgac gaagagatag gaatcatccc agcgaagaac ggcgaaggtt
      421 ccgtcgatcc gctcgacacg aaacccgagg gcggtgagga agtcgatcgt ctttccgaga
      481 tcggggacca cgacctcggc cacaagctgc gcggagttct cggacattgt tcagtcggcg
      541 ccgtagaggg tcatgtcgac gacgcgccag ttgccggcca ccttcttcat cgcgatgtcg
      601 cctttgatct tgccgccgtc gtacttcgtt cctgtgatct ggacctgcgc ctggtcgcct
      661 ttgatctggc cgccggtgac cgtgaccgtc ttcgggtagt tgagggccag accttcgaaa
      721 tactcttttc gctccgacgg cttcggcgcg gttttgggaa cctcttcctc gcggaggtga
      781 ttgtgcgtgg cgtcccagtc gttggcacgg atggcgccga tgagcgcgac gaatgcctgc
      841 cccggctcgc cgcctcctgc cggaagcgcg ctcggtttgg gcatcggcgt gatcgggagg
      901 tcgtaggcga gatgaaacgc gtagggatcg ccgaacagcg tcttttccgg cttggcggtc
      961 gagcagtcgc cggtgatatg cgctgcggtt gatgtcgtgg tcttggcggg gaaggagtcg
     1021 ccgaggtcga tctgcttgcc ggtgtcgagg aaggcgaaga tggcgcagtg gtcggctgga
     1081 gccagcgtga tgatgacgaa gttgccgcgc tcggccttca caatctgcgc gatgagcgcg
     1141 ttcgcgggat cgagagcggc gatcaccgcc ggctgatcga tcttgaagtc cgcgccggcg
     1201 acgacggtga ccggcttctt cggatctttc ccctcgcgct gccaggccat ggcgttcacg
     1261 atctggaact ttgctttgcc gaactggaag gtgccgtgag cgtcgtccgc ggccgcgttg
     1321 ccggccagga gcaggagcac gagagcggga agcgtgcgca gtcggttcat gatggggaac
     1381 atagcactat ggaagaaaga ggggaggcgt gaaaaagacc tccgcgttct ccgcgtctcc
     1441 gcgcgaaacg gttctgcgcc caaccgtatg ttattgtcgc ctctcacgca ggaggttccc
     1501 atgcgttttg agcacgttcg ggcggccctc gttctcgtcg cggctttcgt tctcccggag
     1561 ctcttcaccg ttgcagcgca gtcggtggat gtgatcacgc cgacttccgc gccgcatctg
     1621 cgcgcaagcg acgcgcgcac gccgcacgtg gagacggcgc cgctgccggt gcagaacttc
     1681 accgtctcgc gcggcatcgg gatcctcgag gatgacggga agtcgctcta tcccggcttt
     1741 ccttcgccga ttcatgggca ggagacatgg cgcgccacgc cgaccgcgaa caacaccttc
     1801 atcctgaaga aggttgccta tcagttcgat gacccgtcgg ccggcaccgt gatctacgac
     1861 ggcgcaaaac ggcccatcgt cggctttctc gacgcgaact acattcctcc tcattacacg
     1921 cttcctgcgc cgttcacctt cgcggggaag cagtacacga aactgagcat ctcgacctgg
     1981 ggtgcgattg cgttcggaga tgccgactcg acccaggtca attacgaccc gaccgttgtg
     2041 agcaacatgt tccgcgtgcc gatggtggcc gtgtggtatg agcttttcta ttacccgagc
     2101 aatgcccgcg tcatcgccag gaacaaggcc aactccgtcg tcatcacctg gcagaacgtg
     2161 ttgagccggc actcaccaac gccatgcacc ttccaggcgg agctattttt cgattcagga
     2221 gagattcagc tctcttatca aacgcttccc gtgccggatg gactggttgg gttcagtacc
     2281 ggtacagagg ccgtgaccca tcaggccacc gccgcggtat cgaatcccga cattcccgcc
     2341 tatttacagg ccgccagcgc gtcctttgat aattacggcg ggatcattgc tggcgtcaca
     2401 ctcaaggtca atgcgccgat tcctgtcccg gcggcggggg agtcgtttcg ttacacgctt
     2461 ctcattaatg gtcttgaagt catcgcgttg gagtacacct ccagccagtc catcctggtc
     2521 gtcgttcccg tgccgaaaat ccccgagttt ccgggcgccc agatccaatc gtgggaaacg
     2581 aagttgagcg gcgacacgct ttcggtccgc atgccggcca cgagtctcgt gccgtacctc
     2641 tccacaacgg gcacgagcac ctgggcgatc aagagcacgc gtttcggcgg cgccggtttc
     2701 ctcgaacgaa cgattacgca gactcttcct ctgaatttca ccgtgcggca gtcgctgctg
     2761 cctacgcaga tcgacgcggc tggtgtcgaa gttcctgcgg agatctttca ttacacgccc
     2821 gatgtgtggg ataccgaccg gatccgggaa tcgatcgctg cctatctggt cagccgcggc
     2881 cagagcgtcg actcgttccg ctttttcccg acgagctacg acgacggcct caaacactcc
     2941 aattacgcgg gcacctatcc tcggcaaaag agcgtgaccg gcgccggtca gattcctgcc
     3001 cgcaccgaat gcgactgcca ctatggcgcc gagttctcct ccgtctccga gtcactcggc
     3061 accgaatcaa cgacccagat tgcgctcacc cacgaacttg gccacgagtg tgtgttctat
     3121 gccgactaca aagacgtcga cggagtcgta aagggaccgt ggcgcgatgc cggagtgccg
     3181 tgtttcggtg gcgcgcatcc gaataatgga ttggccaatg agagcatgtt cgccgacagc
     3241 gagtcgggtc tcaagacaat ctccgtcatg ggcggcagtg tgggtggcgt gtatcagttg
     3301 accgcgccgc ggttcggttt cagtcgcgtc gaaatgtact ttctcgggct ggcgtcgcct
     3361 gcggaagtta cgccgatcac gttcgttcag agcagcggga cgagtcagat caccatcgat
     3421 caggtcgtcg cggccaatgg cgtgcgaacg ccgtcgtatg gcggccagca gaaggtgttc
     3481 cgcgttccgg cgttcgtggt caagcgtcag gaagagaccg tcagcgacgc gcagttgcaa
     3541 caactgcaga atctcctgtt ccgctggcag agccgcttct ggcgcgagac cggcggccgt
     3601 gcgcgctcga atacgacgct cgacggcggt tgcagctaca cgctttcagc gagcagcgtg
     3661 aagatcgttt ccgacggttc aacggggagt gtgaatgtat tcgtggagcc ggggtgcacg
     3721 gtgactgcgt cgacgaccga tccgtggatc agcgtcaacg tgaatggagc cggcgccgcg
     3781 agctatgttg tcggccccaa cgctagcggg aacgctcgca acggaaccat cacgatcgcc
     3841 ggtcagccgt ttaccgttaa tcaatacgga aaggggcggc gggaggcggc ggggcattaa
     3901 gtgctgcgag tgcgaaggag aaccgtttca cgcggagacg cggagggcgc agggaagacg
     3961 cttagcgcgt gcgctctggg ggtgcgccat gctcggacaa atactcccgc agcgcctgat
     4021 tgaccacggc tgaatcgtga aaaacatcgg cgagatccgg atcgaggaga atcacgttcg
     4081 tcccttgcat gtaacgctcg tagtacttgc cacgagcgcc accgtgaagg tccatttcag
     4141 gaatgtcgtc ttcttctagt tcgctagttt tctgactcat agaatcttct ttccttcggc
     4201 aacgcgagac gggctgtaat tatccgaaca cgctcaccgc gttgggagtg tgccacgatt
     4261 accagccgtc ctgcggaagt gtaacccgtc gtgaggaaac gaaactcctc gaaggagtgt
     4321 cgcgggtccg ccaaggtccg gtgcagcggg tctccgaaga ccgtcgacgc ttccgcgaac
     4381 gagagtctgt gtttgctcag gttgctgaga ttcttcttgt ggtcccactc gaacttcata
     4441 tttcgaggct acctcggtgc atcgttccat cgtctaagtt ctaccaaccg tagaccattc
     4501 acggtcgcgt agactccacc tcatgctcca tgacgcatgg cacatgctcg aggcgccgcg
     4561 ggagcttccg gaccttccca tcggcaatga caagctgcgg gcgattctgg tccggcgcgg
     4621 catcacgacg ttggatgact accggcgatt tgtcgcgccg tgtattgacg acctgcacga
     4681 tccatcgacg atccacggta ttggcgacgc gtgtgcgcgg atcgaacggg cggtgcggga
     4741 ccgggagacc atcctcatct acggcgacta tgacgtcgac ggcgtcacct ccatcgttct
     4801 tctccaaaca gttcttcgcg ccctcggcgc cgacgcagcg cacgtcgttc cccaccggct
     4861 cgtcgacggc tatgggctga agacggtcgt actcgatcgc gtgctggctg agagagatgt
     4921 ccggctcgtg atcacggttg attgcggcat caccagcgtc gatccggtgc gcgtggcgat
     4981 cgagcgcggg atcgacgtca tcatcaccga ccatcatctt ccaccggaga tgctccccgc
     5041 ggcggcggcg gtgctcaacc cgaaacagga gggatgtgag tatccgttca aggatctggc
     5101 aggtgtcgga gtcgcgttca agctctgctg cgagttgctg cgacggagcg gctcgaagat
     5161 gtcgatcgcg tcgctgttga agatcgcggc gatcggaacg gtggcagacg tcgcgcctct
     5221 catcggcgaa aaccgcacca tcgcatccct cggcctcgca ggtctggccg accctcgcaa
     5281 tcccggtctg cgcgcactga tccgtcgtct ggggatgctg ggacggcctc tacgagccgt
     5341 agatatcggc ttcaagatcg gtccgcgcat caatgccgcc ggacggctgt catctgccga
     5401 gaccgcgatc gatctcttcg ccgccgcgtc ggaagaatcg gcctggacgc tctgcgcgga
     5461 gctcgaccgc ctgaacgccg aacggcagga gatcgagaag ggcgtgcggg cggaggcgga
     5521 ggaagaggtt gcacggatgg ggagcgagca catcttcgtc ctcgccggac acaactggca
     5581 caaaggcgtc ctcggcttga ccgcgggacg gattgcgcag cgttaccacc ggccgacgct
     5641 ggcgatcggg atcgagggcg atgtggcggt gggatcggga cggagcatcg gaacgatcaa
     5701 tctgcacgag cagctcgaat cggtcgcgga gctgttcaca catttcggcg gacacgagtt
     5761 cgcgtgcggg ttctcgttgc cggcgtcgaa cgtcgatgag ctgcggcgga ggctggagga
     5821 acggtttgcc tcgctcgacg acgcactttt tctccgcgaa tcgcaggttg atgccgaggt
     5881 gacgctggcg gagatcgacc gggagtttgt cgccgcacac gagatgctgc agccattcgg
     5941 agcgggcaat ctgcagccgc tgttcctcgc gcgcaacgca tcggtcatcg caacgcgaga
     6001 gttcgccgag gattgttgcg agattacgat cgacgacggg acggggaggg ggctggcggt
     6061 gctttggcca tcggcgtgcg cccttgcacc ggatctgctg cgcggcgcga ccgtcgattt
     6121 gttgttccag atcgagcccg atagttactc gccgacgggt gcgaagctgg tgattgttga
     6181 cgcgaggagt gcgagcgtct cgctcgcggc cagcggggcg tctcgccccg gtggttagaa
     6241 tttcggataa gagccaagcg cacggaacgt cagtcatcgt caggccacca ctcgggcgag
     6301 gacgcccgac tgcccgcggg cgagacgccc acactctacg gaatagcctt cacccttaac
     6361 gagttgcggg cgacgtgcag cgcacgatcc gcatccttcg cgtcgcactt cccctcgtct
     6421 tcatcggctt tgtgctcgtc atcgcgctga gctggactcg cggaaaaccg aagcgggaca
     6481 aggctgggac gattccggtg acgtcgacga tccgcccgat cgacaagcct gccatcgtgt
     6541 cgacgacctt cgaagatacg cagacgatca acggacggct ggcctctcac atccgcgccg
     6601 agcgtgtcgt cgcctttcag tccggctgga acaccctcga gaacgtgcag atgacgatct
     6661 accggcccaa agggctgacg tacgaggtcg tttgtccgca ggcgcagttc aatagcaaca
     6721 cgaaggaatc cgacgccaag ggcggggtgc gcgtcacgtc gagcgacggc gtggagatca
     6781 ccactgccga gatccacttc gacggtgtcc acctgacaaa tcacgtcgcc gtgaatttca
     6841 agatcgaccg ctggacgggg agtggtggcg cactcgacat ggacgtgccg ggcgagatgg
     6901 tgcggctcta cgagaaggtc gatgccacga tggcaccgca aactcctgcc gagtcgccgc
     6961 tgaacatcaa agggcaagag ggggttttcc ggcgcaagga aaactacgcg gacttcacca
     7021 aggacgtcgt ggccacgcgc gacagcgatc gcttcaccgt cgatcacgtg gtcggccgct
     7081 tcggtgccga tcacaaggct ctcatggcgc tggaagggca gggacatctg acgatcgtca
     7141 tgaccggcga ttcggcgttg gcagggaaga gttccgagac cgagggacgg aagacgatca
     7201 ccgcggaccg tttcttcagc gagatcggcg cgacgggcga gatcagcgcg atgaactttg
     7261 tcggcgacac ggcacccgct catgcggtga tcgaaggtcc gcccaatcgt gatctcgtgg
     7321 cgcacacctt ccgcgtcggc atcgccaaca agcaggtcac cgagatgaag gccgacgaca
     7381 acgtgacgat gagggagacc gcgccgatca agcgcctgat gacatcggga cacctcgtcg
     7441 tctacttcga tcctgccacg caccgtgctg ccagcgctgc gctcgacggc aacgttcact
     7501 accaggatcc aaagaatgac gcgcattcgg tgcgcgcgaa ttacgacatc accggcgatc
     7561 acgtcacgct gacgggcgag ccgggattct tcccttccgt cgtctcggat gggcagacgc
     7621 tgaaggcgaa gctgatcgag ttctcaccgc gcgcggggac ggcgcgaggg accggcgagg
     7681 tgatcgcact gctcgtatcg aagcaggcga acggtgcgcc ggccgcagtc agcgccgacg
     7741 ccaccagcat cttcccggcg ggcaagccgg tcttcgtgaa ctccgacacg gtgatgatgc
     7801 agcaggccac gaaggtgggg atcttcagcg gtcacgtgcg tgcgtggcag gaaacgaaca
     7861 cggtcttctc gcaggagctg caggtccagg gcgccggcga tcagatcacc gcccgcggca
     7921 atgtgaggac gctgctctac aacacgagca cgaaagcggc cgccgaggtg cgcaaggtgc
     7981 cgatgcaatc aacgagcgat gtgctcatcg cacgcaaggc ggaccggcgc atcgatctca
     8041 acggcagcgt gcagatcgat gacggcgaag gacggcattt cagttccgag cacgcctcgt
     8101 tcttcttcga tgccgccaag aagatggatc acatcgacgc ccaggaaaag gtcgttctcg
     8161 tggagaaggc caccatgcgc cgtggcacgg gcgacaaggc cacatacaac gtcgcgcagc
     8221 gaatggtcta cctgatcggc tctccggccc tcgtcaccac gccgaccggc tcactgccgg
     8281 gaaagaagat ctcgctcgac ctggagcgca acaaggtcga cgtcgtggag ccgatgcagg
     8341 gaacgtacaa gccgccgcct tgattagcag cgcggtccgg gtaggcgaac ccgctaccac
     8401 agagacacag aggtcgcaga ggtcaactct gagagctcag tgcctctgtg gttcaatttt
     8461 ttttgaccgt cacagctacc gccgcgcaat acgctaacat cccctccctg aatgtctgac
     8521 cgttcttcca ctgtgccctt caccacatcg gaccgcgagc gaacgccgga cgcgccgttg
     8581 cgtctggccg cggagcatct cgtcaaggtc tacaaacggc gtcaagtggt caaagacgtc
     8641 tcggtcgaga tcaagcaggg tgagatcgtc ggactcctcg ggccgaacgg tgcgggaaag
     8701 accacgacct tctacatgat cgtcggtctg gtccgtcccg acgatgggaa ggtcaccctc
     8761 ggcagcgacg acatcaccgc gcttcccatg tatctgcgtg cgcagcgggg gatcagctac
     8821 ttgccgcagg aagcatcggt cttccgcaag ctgaccgtcg aagagaatct gctggccgta
     8881 ttcgagacga tggatctgcc gctggcggag cggaaacggc gcacgcggat gctgctcgag
     8941 gagttcggga tcacgcacat cgccaagaac cgggccttct cactttcggg cggcgaacgg
     9001 cggcgggtcg agatcgcgag aagcctcgcc atcaacccgg cattcatctt gctggatgag
     9061 ccgtttgcag gaatcgatcc aattgcggta tttgagatcc aacgaatcgt ttcgcagctt
     9121 cgtgcccgcg ggatcggcat cctcatcacc gatcacaacg tacgcgagac tctgaagatc
     9181 actgaccgcg cttacatcat caaagagggt gagattttcc ggcagggagt tcctgagagt
     9241 ctttcggcag acgcggaggt ccggcggatt tatctcggcg aatctttcaa tctgaactga
     9301 cgcatgctcc caaggaaagc gaagtaacct gtcgatccag ctatggcact agaacagaaa
     9361 ctacacctca agctatccca gaaactgatc atgacgccgt cgctgcagca ggcgatcaag
     9421 ctgctgcagt tgtcgaaact cgagctccag gaagtgctca accaggaact gctggaaaac
     9481 ccgctgctgg aggaatccgc ggacgaggcc aagcaggaag aagcggaggt cgaagcgcag
     9541 gagaaaacgc agaccgagga agaagcgaaa gccgcggagc ccgcgccgga gaaggagaag
     9601 gactccttcg acgaaatcga ctacgacgcc tacttccagg actacatcga gtacggatac
     9661 aacccgcgga tgggggagga ccacgaagag ttccccatcg agaacactct cacgcgtcct
     9721 ccgaacctca ccgatcacct ggcgtggcag ctcggcatga gcgatgcctc accggcggtc
     9781 aaggacattg gcgccttcat catcggcaac atcgacgagg acggctacct ccgcgctacc
     9841 agcgaagaga ttgccgcggc cggtccgtac gatccggctg acgtcgagaa ggccatcgct
     9901 gcgattcagt cgctggatcc gatcggcgtc ggagcccgcg atcttcgcga gtgtctgctg
     9961 ctgcagctcc agtttctcga ggtcgacaac acgctggtcg agtcgatcgt ccgcgaccac
    10021 tgggacctgt tcatgcagcg tcacttcgtg cagatcgcca aggctctcgg catcgacatg
    10081 aagacgctgg agggcgtcgt cgagatcatc aagcacctcg acccgaaacc ggggcgcaag
    10141 tactcgaacg agcgcgccat ttacgtcgag cccgacgtct acattcagaa ggtcggcgac
    10201 gattacgtca tcgttctcaa cgaagacggc atgccgaaac tgcgcatcaa cggcagttac
    10261 cgctcgatgc tgaactcgat ggactcgaag tcggacggcg agaccgtcaa ctacatcaaa
    10321 gacaagatcc gctcggccgt ctggctcatc aagtcgctcg accagcggca gcgcacgatc
    10381 tacaaagtgg ccgagtcgat cgtgaaacac cagcgcgagt tcctggagaa gggtatcgac
    10441 tacctgcgcc cgctcgtcct gcgcgacgtc gccgacgaca tccagatgca cgagtccacc
    10501 gtctcccgcg tcgtgtcgaa caagtacatg cacacgccgc gcggactgtt cctcatgaag
    10561 tacttcttcc acagcggcat cgactccgat accggcgaag acatctccag cctcacggtc
    10621 aagaagaaga ttcagggctt catcgacggc gaggatccac ggaaaccgct gtcggactcg
    10681 aagatcatga agatcctgaa cgatgaaggc atcaatattg cccgtcgtac ggttgccaag
    10741 taccgcgatg agctgaacat cccctcatcc acggaccgca agcagatctt ctgaagccgg
    10801 ttttctgaag tcacaaggga ggccttgaat ggcagcagtc acccccgaga tcgtggggcg
    10861 taactttgaa gtcacacccg acatccgcgc gctgatcgaa tcgaagctcg gtcgtgtcga
    10921 ggatcggctg ttccaggacg tcagcagcgt acgcgtcatg ctgcacgtcg agaagttccg
    10981 gtacacgtgc gagatcctcg ttgtcgccaa ggatcacgac gccaaagccg tgcaggagtc
    11041 agagacatcg atggccgatg ccatcaacgc cgccgtcgac cacatcaagc gccaggcgca
    11101 gaagagccgc gagaagatcc gcgaccacca ccgcggcggg ccggagccgg ttcccgaggg
    11161 gaacagccgt gtttcgtagc agccaggctt ccgcggagat acgatagcgc tcatggctac
    11221 gacaattgcc ggacggcatt acacagtcac tgaggacatc cgcactctga tcacgacgaa
    11281 ggtcggaaag atcgtcgaga aactattcaa cgatgtcatc gacgtacgcg tcgttctgca
    11341 ggtcgagaag taccggaaca tctgcgagat tctcatcaaa gggaaagatc acgatgtgaa
    11401 gtcggtgcag gaatccggcg aatcgatgca ggacgcgatc aacaaggcgc tcgatcacgt
    11461 caaacgccag gcccagaaga accggaagac gattcgcgat catcatcgca aagacggtgt
    11521 gctcaacaag agcgtggctg gtcaggtgac ggagtgggcg gtgcaggtgc tcgagcccgg
    11581 caagctgcgc aaaggcggca aggcccgtct tccccggatc atcaagacaa acagcctggc
    11641 catcctgccg atgacgatcg aggatgccgc ggagtcgctg gacggatcga agaacgagtt
    11701 catcgttttt cgcgatctcg acaacgatcg catctccgtc atctacaagc ggcgcgacaa
    11761 caacctcggg ctcatcgcac cggaagtgtg atcgaaagga cccggctact aacgctttga
    11821 tctgaagcct tcgggcgggc cggtcgttgt gcagtcaacc ggcccgttgc cgtgcttacc
    11881 gggaagccgg ttgaataagc ttcaactgtg cggccatctc gtacaccttg tcgaggtcgg
    11941 cctgcgtctt gcggtcgatg cgctctctgc cgccggggtc cacagcgatg acgacgtcga
    12001 gattgctgtc gaatcgcggc ggcagccgtt tagcgtcgta ggtgaatgaa tacgtgtgca
    12061 gcatgctatc gccggcgccg gcgttgttcg ccgcgcccgc gtaatcgtcg acggagccac
    12121 cggcggagtt cgcagagcca ccgccttcgt tcatattcgt cagcgctccg cctgagggag
    12181 tgcgcagggc gccgcgcacc ggggtatcgg tcttggacat cgcggacgga ggtccaagga
    12241 tgatgacgag ccggccgcgt tccgtgcgtg agccaggtgt ctttcccgtg gtgaagtact
    12301 tgtcggcctg ggccgcggca aaattcacct ggtcggcgaa gtcggggccg cgtctggcga
    12361 gaaagtcttt aacgaacttt tcggcctcgt cgtccgtctt gacgcttgcc cactgggatc
    12421 gttcggaggc cgtcatcaga tatccctgag gggttttgtc ccatcccttg tacttcgaga
    12481 gaccgccagc aaatgccgcg acggccgcaa aacagaggac tgcagcgccg atcagatgcc
    12541 tttttctcat ggatgcccgc cttgatgtcg gattatcgac cgggattgtg acacagatag
    12601 cttggccgcg gatgtccatc cggcaggggc gtgcctcgta atcgccactc tgagtcggaa
    12661 agttaacccg cgggatcaaa gcgcactcgt tgatcgtttg atcaaaccag agacgagcag
    12721 tattcttacc caattggcta ctaggaggct ctcgatgaaa acacgtctgc tgttcgcggc
    12781 aggaattctt cttttttcag catctctact tgctgatgga ttggtgaagt tcaagggctg
    12841 ggacagttcg ccacagggat atttcatgac cagggccgag cgccagcagt gggcgtccgt
    12901 ccggaccgac gatgaggcac agcgattcgt cgacacgttc ctcgccagcc gcggccctgg
    12961 gtttgcggca gaggtggctt cccgcgtgga gcaggcggat aagcacttga cgatcgaagg
    13021 tacgcatctg ccggggtcga agacccttcg cgggaagctg atcattctcc tgggcgcccc
    13081 tacctcctac gacgccagcg acttcaccga ccgaagctcg gtgcatcaag acagcccaat
    13141 catggccaac gcgctgagcg gaggctccag cggcgacagc ggcggcggtg ggaagggagg
    13201 cgccgacctg ggtacgggtg gctccgatat gagcgaagga gcgagaacga tgggcgccgg
    13261 acgcacgatc cggagcttcc acttcggatt cgcgtcgacg ccggcaggac ccatggaggt
    13321 aaccatcaac gctgacacga acaccgggaa ggaccgcccg cgcggcaagg acgactccaa
    13381 gcgcctcgac gccgcgttcg aagcggccgc acaggcctcg atcaaaacga agtagacagc
    13441 acctcgatca ctctccggcc gctgccgtct cgatgacggc agcggtttct tgttgcattg
    13501 attcgcttac cataggcccg tgaccaacca ggccgccgaa gacttcatcc cgaccacagt
    13561 cggcgttctc cgttccggcg acctcgacga tctctccctc agactcgtca cgggggagga
    13621 cggtctcgac cgtgtcattc tccgtccgcg cgtgcagaag cccggcctcg ccttcgccgg
    13681 ctactatgaa tacatcaagc agtggcgcgt ccagatcatc ggcgagtccg agatcaagta
    13741 cctgctctcc cttccgccgc ggcttcgcga gaaacgggtg cgcgacgtgg ccgcgctcga
    13801 cgtctcctgc ttcatcgtca cgaagggaat cgcgccgctc gaggagttcc gccacgagtg
    13861 cgaaaagcgc tccgtgccgc tcttttcgac acaagccatg tcgtcgacgt cgatcacgcg
    13921 cacgacgtac ttcctcgaag agacgatggc gccgaagctg acgatgcatt cgggccttct
    13981 cgacgtctac ggtatcggcg ttctcctcct cggcgacagc ggcatcggca agagcgagtg
    14041 cgcgctcgat ctgatctatc gcgggcaccg gctgatcgcg gacgacatgg tggtcatcaa
    14101 gcgccatccg aacgatgtac ttctcggcta ctcgaacgac ctgctgcggc atcacatgga
    14161 gctgcgcggc atcggcatca tcgacatcaa ggatctcttc ggcgtggcgt cgacgcgcga
    14221 tgtgaaaccg atcgatctgg tggtgaggct ggagaagtgg gtcgagggga ccgagtacga
    14281 ccgccttggc atcaccggcg agaccttcga gctcctcggc gtctccaaac cctacgtccg
    14341 tctgcccgtg gcctcggggc gcaatctcgc gcttctcgtc gagatcgcgg cgcgcaatca
    14401 cctcatgaaa ctgcagggcc acgactcggc ccgctcgttc ctccagcgca tcgacgagca
    14461 gatcgcgaag caggggagtg aggaaggcag cgtgctgccg agctctaatc ccgaggagaa
    14521 agccagctcc gagctgcgca gcatgagcac gaagatgcgc ttccaggccg acgtgatcga
    14581 acggacgcga aggcagagat aagtcctatc aggatgccga ggttgagtgt catcccgagt
    14641 gataggccaa ccgagattgt gtcatcctga gactgccgaa gccgcccg
//
LOCUS       DATLVW010000412         6424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_144351, whole genome shotgun sequence.
ACCESSION   DATLVW010000412 DATLVW010000000
VERSION     DATLVW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144351"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VNN25_12445"
     CDS             <1..510
                     /locus_tag="VNN25_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92382.1"
                     /translation="PRPDYLATAEEQIASERIAGIDKQLGNSNDPESLALRQRADRLA
                     GALTWRLETEYNERLTAAHVHLSELNAPVDTLTRQYDAFVRTRQAATHSYVGYDVQIA
                     RLRERVEEALQRVDVLIARQGHMIETVAIDQLEARRERLAAQQTEARFGIADSYDRAA
                     RAQSDAGGH"
     gene            510..4061
                     /locus_tag="VNN25_12450"
     CDS             510..4061
                     /locus_tag="VNN25_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92383.1"
                     /translation="MPRFAIPIVIAPVFMVGCAARPVKPVGTAGTLAELRNVRPDVQE
                     VKIERGLDQAMQQYRRFLEEAPETAMTPEAMRRLADLQLEKQFGIRPGNTKPLEMAAP
                     KPAQVLSGSRVGTPNPGAAGAPLHESDQDFERRTTAEAGILAGSNASASPADAVRAGA
                     DAEGPLEAIALYNRLLTEYPSYKNSDQVLYQMARAYDELGRTDEAIDTMERLIRTNPH
                     SSHLDEVQFRRGEYFFTRRRYRDAESAYSGIVKLRHASEFYELGLYKLGWTFYKQEFY
                     EEALQRYIALLDYKVSIGYDFDQKHDEDDDRRVADTFRVISLSFSNLGGPETAPEYFS
                     KFGNRSYEDRIYSSLGEHYLGKLRFDDAAKTYKSFVALYPFHRAAPRFSMRVVDTFTQ
                     GGFPKLVLESKREFASKYGLKSEYWRHFKPEESPEVLAYLKTNLKDLATHYHAQYQNT
                     NGADEKLTNYREASQWYGAYLESFPKEADSASVNYQLADLLLENADFGEAAKQYERTA
                     YGYAPHPQSAGAGYAAIYAYREQLKVADKKQQDAVKRDTVASSLKFADTFPQDEHAAA
                     ILGAAADDMYEMKDYKAAIEADQRVLDKYPSAAASIRRSAWIVVAHGSFEVAEYPRAE
                     LAYTQVLAATPESDKSRAAFVDNLAASIYKQGELANEAKDYRAAADNFLRIRTAAPSS
                     AIRETAEYDAGAALIRLQDWKAAVDVLEAFRTTFPKNKLQLEATKQIAYAYRQSGELS
                     HAAGEYERIASQSDDPALRSEALLDAGDLYTQSNSRERALEAYNRYVKEFPKPVETAI
                     ETRLKIAEMYKTAHDETLYHQQLKEIVSADAAAGSERSGRTRTLAARSALVLAEQLYE
                     NFAVVKLRQPFETSLQDKKQRMDATIAAMGQLANYGIDEVTAAATYYMAETYSNLSRS
                     LLESERPDDLKPEDLEEFKNKLDEAAFPFEEKAINVHEKNMELLRAGTFNSWTEKSLS
                     RLTELMPGRYAKHETSSGFLAAIDNHVEGSPASQVSNSSKSANAITQGAGVTDEMRAN
                     YESAVGMLKEERYEPGIALLLKMTEKMPALTAAHIDLGIAYARTGDLDRAEASLNKAL
                     QLDPKQPVAYNELGMVQRRKTQFAKARASYEAALAQSADFQYAHRNLAILCDLYLGDY
                     TCAMEHYEAYGRIVPGDAEVVKWIADLRNRAKKREER"
     gene            4058..4546
                     /locus_tag="VNN25_12455"
     CDS             4058..4546
                     /locus_tag="VNN25_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92384.1"
                     /translation="MRKASVHWISIGVLALVPTAVLAQQAAQPKKEAARDKTEKSHAK
                     TQEVKKEEEKKQTEEPQPKTEDEAKLTSVPGGKALGMSILGNQEAPKALVIVPWKSSE
                     LANGPGVSTLLDDSRRPVDKEVFMRMLSYYEIRSETPRPGGATTNGNAAAPAIEAQRR
                     KQ"
     gene            4591..5280
                     /locus_tag="VNN25_12460"
     CDS             4591..5280
                     /locus_tag="VNN25_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXH92385.1"
                     /translation="MIFSLALLSVFSVGMIIEKHRRFSSASGQSDKFKPEFKKFLHGG
                     DVQNLIEAAGQQQNSHVAQVVSAGIAEYDAVAQNGGDPTASLELVTSALRDSISETLI
                     QLKRGLGFLATIGSTAPFVGLFGTVVGIINAFRSIAATGSGGMSVVSGGIAEALVSTA
                     LGIFVAIPAVVAFNHFTGKMETFHVEMNRASSQLVNRLFKIPELKGLDVEEVEVKAPS
                     VKKDGPAYATR"
     gene            5291..5683
                     /locus_tag="VNN25_12465"
     CDS             5291..5683
                     /locus_tag="VNN25_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXH92386.1"
                     /translation="MQAAINITPLVDVVLVLLIIFMVMAPQMRKGPDVNLPNTTKPNS
                     QGDERGRILVSIDDSGGLWINDQAVAAEHFDETLRAAVGAEANPKIVIRGDAKLNFRE
                     VREVMVAIEQAGFRGVGLIAKRTGAEGK"
     gene            5688..6152
                     /locus_tag="VNN25_12470"
     CDS             5688..6152
                     /locus_tag="VNN25_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXH92387.1"
                     /translation="MQFNSNDSGSSLQSEINITPLVDVVLVLLIIFMVVVPLLMEGYD
                     VDIPRASADTSAQVAESQLILSIGSATCPILAPPAGEGIPKNCQVTLADQKIPVAELP
                     SRVAEILGRRPPEQRALFLAVDDRLNYEAVLRIIDLAKSGVEDLKIEFVAAN"
     gene            6174..>6424
                     /locus_tag="VNN25_12475"
     CDS             6174..>6424
                     /locus_tag="VNN25_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92388.1"
                     /translation="METKIIFKTSGAPTARSMAKSTAIHVFLLAMLAVIPAQALFRSE
                     SPNKELDIVFYRPPEIALNTRTVSRPMPRGVAGSPPGAPA"
BASE COUNT         1423 a         1911 c         1932 g         1158 t
ORIGIN      
        1 ccgcggccgg attatctagc gacggcggag gagcagatcg ccagcgagcg catcgcaggc
       61 atcgataagc agctcggcaa ctctaacgac cctgaatctc ttgcgcttcg tcagcgcgcg
      121 gaccgcctgg ccggcgcgct cacgtggcgg ctggaaactg agtacaacga gcggctgacc
      181 gcggcacacg tacacctaag cgaactgaat gcacctgttg acactctgac tcggcagtat
      241 gacgccttcg ttagaacgag gcaggcggcg acgcacagtt acgtcggtta cgacgttcag
      301 attgcacggc tccgcgagcg cgtcgaggaa gccttgcaac gtgtcgacgt attgatcgcc
      361 cggcagggac acatgatcga gaccgtcgcc atcgaccaac tcgaggctcg ccgggaacgc
      421 ctggccgccc agcaaaccga ggcgcgcttc ggcattgccg atagctacga tcgagctgca
      481 cgagcccagt ccgacgcggg gggacactga tgcctcgctt cgccattccg atagtgatcg
      541 cgccggtttt catggtcggt tgcgccgccc gtcccgtcaa gccggtgggg acggcgggaa
      601 cgctggcgga gctgcggaac gtgcgtccgg acgtgcaaga ggtgaagatt gagcgaggcc
      661 tcgatcaggc catgcagcaa taccgccgct tcctcgagga ggcaccggag actgcaatga
      721 ctccggaagc catgcggcgc ctcgccgacc tgcaactcga gaagcagttc gggatacgtc
      781 ctggcaacac caagccgctc gaaatggccg ccccgaagcc cgcccaagta ctctcgggct
      841 cgcgggtagg cactccgaat ccgggcgctg ccggcgcgcc attgcacgaa tccgatcagg
      901 atttcgagcg gcgcacgacg gcagaggccg gaatcctggc gggcagtaac gccagcgcct
      961 cgccggcgga tgcggtacgc gctggcgcag atgccgaggg tcccctcgag gcgatcgctc
     1021 tctacaatcg gctgctcacc gagtacccca gttacaagaa cagcgatcag gttctctatc
     1081 agatggcgcg ggcgtacgac gagctcggcc gcaccgacga ggccatcgat acaatggagc
     1141 gcctgatccg cacgaatcct cattcctcgc acctcgacga agtgcagttc aggcgcgggg
     1201 agtatttctt cacgcgaaga cgctaccgcg acgccgagag cgcctattcg ggcatcgtca
     1261 aactgcgcca tgcctccgag ttctacgaac tcggtctcta caagctcgga tggacgttct
     1321 acaagcagga attctacgaa gaggctctgc aaaggtatat tgccctgctc gactacaaag
     1381 tatcgatcgg ttacgacttc gatcagaaac atgatgagga cgacgatcgc cgcgttgcgg
     1441 atacgttccg ggtgatcagc ctgagcttca gcaacctcgg cgggccggag accgcgccgg
     1501 aatacttctc caagttcggg aatcgctcct atgaggaccg catttactcc agtcttggtg
     1561 agcactatct cggtaagctg cggttcgacg atgctgccaa gacgtacaaa agcttcgtag
     1621 cgctttatcc gttccaccgt gccgcgccac gattcagcat gcgcgtcgtg gatactttca
     1681 cccagggggg gttccccaag ctggtactgg agtcgaagcg tgagttcgct tccaagtacg
     1741 gcctgaagtc cgaatactgg cgacatttca aacccgaaga gtcgccggaa gtattggcct
     1801 acttgaagac gaacctgaaa gacctcgcaa cgcattatca cgcccagtat cagaacacaa
     1861 acggcgcaga cgagaagctc acgaactatc gcgaggcaag ccaatggtac ggcgcatacc
     1921 ttgagtcctt cccgaaggaa gctgattcgg cgtccgtcaa ctatcagctt gcggacctgc
     1981 ttttggagaa cgcggatttc ggcgaggcgg ccaaacagta tgaacgcacg gcgtacggat
     2041 atgcgccgca cccgcagtcg gcgggtgcag gttacgccgc gatctatgca taccgcgagc
     2101 agctgaaggt tgccgacaaa aagcagcagg acgccgtcaa gcgcgatacg gtcgcaagct
     2161 ccctcaaatt tgctgacacg ttcccgcagg acgaacacgc ggcagcgatc ctcggagcgg
     2221 ccgctgatga catgtacgag atgaaggact ataaggccgc catcgaggcc gaccagcgcg
     2281 tccttgataa atatccgagc gcagccgcat cgatccggcg ttccgcgtgg attgtggttg
     2341 cgcatggctc gttcgaggtc gccgaatacc cgagggccga actcgcctac acacaggtgc
     2401 tggcagccac gcctgagagc gacaagtctc gcgccgcttt cgtcgacaac ctagccgcct
     2461 cgatttacaa acagggtgag ctcgccaacg aggcgaagga ttatcgtgcc gccgccgaca
     2521 acttcctcag gattcgcaca gcggcgcctt catcagctat ccgtgagacg gctgagtacg
     2581 acgcgggagc cgcgctaatc cggctgcagg actggaaggc ggcggtggac gtgcttgaag
     2641 cgtttcgcac gaccttcccg aagaacaaac tgcagctcga ggccaccaag cagatcgcct
     2701 acgcctaccg gcagagcggc gagttgtcgc acgctgccgg cgaatatgag cgcattgctt
     2761 ctcagtccga cgatccggcg ttacgcagcg aggcgctgct cgatgccgga gacctctaca
     2821 cgcaatccaa ttcccgagag cgcgccttgg aagcatacaa ccggtacgtt aaagagtttc
     2881 ccaaaccggt agagacggcc atcgagacgc ggctcaagat cgccgagatg tacaagaccg
     2941 cgcacgacga gacgctctat caccagcagt tgaaagaaat tgtaagtgcc gatgcggccg
     3001 ccggatcgga aaggagcggc cggacgcgga cgcttgcggc gcgctcggcc ctcgtcctcg
     3061 cagagcagct ctatgaaaat tttgccgtcg tgaaactgcg gcaacccttc gaaaccagcc
     3121 tgcaggacaa aaagcagcgt atggatgcca cgattgccgc catgggccag cttgcgaact
     3181 acggaattga cgaggtgacg gcggcagcga cgtattacat ggccgagacg tactccaatt
     3241 tgagtcgctc gctgctcgag tcggagcggc cggacgactt gaaacccgag gatctggaag
     3301 agttcaagaa caagctcgat gaggcggcgt tcccgttcga ggaaaaagcc attaacgtgc
     3361 acgagaagaa catggaattg ttgcgcgccg gcactttcaa ctcgtggact gagaagagtc
     3421 tcagcaggct caccgagctg atgcccggcc gctacgcgaa acacgaaacg agcagcggct
     3481 tcctcgccgc gatcgacaat catgtggagg gctcgcctgc gtcgcaggtc tccaactcct
     3541 cgaaatcagc caacgcgatc acccagggcg ctggcgtcac cgatgaaatg cgcgccaact
     3601 acgagtccgc tgtgggcatg ctaaaggagg agcggtacga gccgggcatt gccctactgc
     3661 tcaaaatgac cgagaagatg cctgctttga cggcggcgca catcgatctc ggcatcgctt
     3721 atgcgcgcac cggcgacctg gaccgcgccg aagccagcct gaacaaggca ctgcaattgg
     3781 atccaaaaca gcctgtcgcc tacaatgaac tcggtatggt gcagcggcgc aagacgcagt
     3841 ttgcgaaggc gcgcgcaagt tatgaggcag ccctggcaca gtccgccgac tttcagtacg
     3901 cccacaggaa ccttgcgatt ctgtgcgact tgtatctcgg ggattacacc tgcgcgatgg
     3961 agcattacga ggcgtacggc cggatcgttc caggtgacgc ggaggtcgtc aagtggattg
     4021 ccgatcttcg caaccgcgca aagaaacggg aggagcgatg aggaaggcta gcgttcattg
     4081 gatttccatt ggggtccttg ccttggtgcc cacggctgta cttgctcagc aagcagcgca
     4141 gccaaagaag gaagctgcac gggacaagac cgaaaagtcc cacgcaaaga cacaagaggt
     4201 aaagaaggag gaggaaaaga aacagactga agagccgcag ccaaagacgg aagacgaggc
     4261 caagttgact tccgtccccg gaggaaaggc gctcggcatg tctattttgg gcaatcagga
     4321 agcgcctaaa gcactggtca tcgttccctg gaagagctcg gaactcgcca atggccctgg
     4381 cgtttcgacg ttgcttgatg actcgaggcg gccagtagac aaagaagtgt tcatgcgaat
     4441 gctcagttac tacgaaattc gatcggaaac accacgcccc ggcggtgcga cgacgaatgg
     4501 gaatgcggca gcgcccgcca ttgaggcgca aaggaggaag caatgagttt agctgaactg
     4561 ctacgacacg tcggtatatt cggaggcgcg gtgatctttt ccctcgccct gctctctgtt
     4621 ttttcggtgg ggatgatcat agaaaagcac cgccgcttct cttcagcctc gggccagtcg
     4681 gataagttca agcccgagtt caagaagttc cttcacgggg gagatgtcca gaatctgatc
     4741 gaagccgcgg gtcagcagca gaattcccac gtggcgcaag tggtctcggc cggcatcgcc
     4801 gagtacgacg ccgtggccca gaacggaggc gatccgacgg cttcgctcga actcgtcacg
     4861 agcgcactcc gggactctat ctctgaaacg ctgattcagc tgaagcgggg tcttgggttc
     4921 cttgccacga tcggctccac tgcccccttc gttggtcttt tcggcaccgt ggtcggaatt
     4981 attaacgcct tccggagcat cgcggccaca gggtctggcg gaatgtctgt ggtttcaggc
     5041 ggcatcgctg aggccctggt ctcgacggcc ttgggtatct tcgtcgcaat tccggccgtc
     5101 gtcgccttta accacttcac aggcaagatg gagaccttcc atgtcgaaat gaatcgggct
     5161 tcgtcccaac tcgtgaaccg cttgttcaaa attccagagc tcaaggggct tgacgtcgaa
     5221 gaggtggaag tcaaggcgcc ctcggtcaag aaggatggcc cagcctatgc gactcggtga
     5281 gccagctcgg atgcaagcgg ccatcaacat cacgccgctc gtcgacgtgg tgctggtcct
     5341 gctgatcatc ttcatggtga tggcgccgca gatgcggaag ggacccgacg tcaacctgcc
     5401 gaacacgacg aaaccaaact cgcagggcga cgagagaggc cgaatcctgg tctccatcga
     5461 cgattctggc gggctgtgga tcaacgacca ggccgttgcc gccgagcatt ttgacgaaac
     5521 gctccgcgct gcggtgggcg ccgaagcgaa ccccaagatc gtgatccggg gcgatgcaaa
     5581 gctcaatttc cgcgaggtcc gcgaggtcat ggtcgcgatc gagcaggccg gcttccgcgg
     5641 ggtggggctg atcgccaagc gcaccggcgc agaaggaaag tgacatcatg cagttcaaca
     5701 gcaacgattc tggctccagt ctgcagtctg aaatcaacat cactccgctc gtcgacgtgg
     5761 tgctcgtgct gctgatcata tttatggtcg tcgtaccgct cctgatggaa ggttacgacg
     5821 ttgatatccc ccgagcctcc gccgatacat cggctcaggt tgccgaatcc cagttgatcc
     5881 tgagcatcgg ctccgcgacg tgccccatcc tcgcgccgcc agcaggggaa gggataccca
     5941 agaactgtca ggtgaccctg gcggatcaaa agatccctgt ggccgaattg ccttcgcgag
     6001 tggcggagat tctgggccgc cgaccgccgg aacagcgcgc cctgtttctg gccgtcgacg
     6061 atcgcctgaa ctatgaggcg gttctacgca tcattgatct cgcaaaatcg ggcgttgagg
     6121 atttgaagat cgaatttgtc gcggccaatt gaggtggttg attggtgaca accatggaaa
     6181 caaagatcat attcaaaaca tccggggctc cgacggcccg cagtatggcg aagagtacgg
     6241 cgatccatgt cttcttactg gctatgctgg cggtcatacc ggcacaggcg ctgtttcgca
     6301 gcgagtcccc caacaaagaa ctcgacatcg tcttctatcg tccgccggag atcgcactca
     6361 acacgcgaac cgtttcgcga ccgatgccgc gaggcgtggc cgggtcccca ccaggggccc
     6421 ctgc
//
LOCUS       DATLVW010000413         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_16966, whole genome shotgun sequence.
ACCESSION   DATLVW010000413 DATLVW010000000
VERSION     DATLVW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16966"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VNN25_12480"
     CDS             <1..1458
                     /locus_tag="VNN25_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HXH92389.1"
                     /translation="AIIVAYLGGVVAIGFWCRKRQKTTRHYFLAGRDLPWWAISGSIV
                     ATETSAITFISVPGLIYARGGNFTFLQLVFGYLIGRVVICLLFIPAYFRRNLLTIYEL
                     LRSRFGGSVRSLAASLFIIMRTIADGVRLLLTSVVMSTVWHAFLPKTDMATAAAISVI
                     CIGGVMLLFTTVGGMEAVIWTEIVHVGVYIIGAGAAIAILVHAIPGGLSGAIAAGSAA
                     AKFRLFDFSLDFTRPYVFWAGVIGGAFLNMSTHGTDQYMVQRYLCTTSARKAQAALLV
                     SGMVILVQFAVFLFIGVLLFGYYHPASLPGYATGPAAAPFMAPDQVFPDFIVHHVPIG
                     LSGLLVAAVLAAATSPSVNSIAATAVSDLYQPLVRGRSDGHYLRVSRILTVVAGVAQI
                     GVALALQGQARSAVDTALGVASLINGPILGVFLLGSMRRAGRIGALCGMSAGIAAVTA
                     VFAATHIAWPWYTVIGSLTTFAVGTLIPEREHAPI"
     gene            1445..>3370
                     /locus_tag="VNN25_12485"
     CDS             1445..>3370
                     /locus_tag="VNN25_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HXH92390.1"
                     /translation="MRRFSIAVAALLAGCLTTQLAARFDPDRLRAIDQTIETAIAAHK
                     VPGGVFHLERDGAIYEKAYGNRALVPAVEPMTVDTIFDAASITKVVATTPSVWLLIER
                     GKIELDAPVSRYIPAFKGGWRDEMTIRHLLTHTSGLRPDLDLTPAWSGYDTAIRMAIA
                     EEPRQRPGYVFRYSDINFELLGEIVRRVSGEPLDVFAKREIFEPLKMHDTSFRARSAD
                     GSSAVPPAARRQDTNRIAPTEQTPEGMLRGVVHDPTSRRMGGVAGHAGLFTTAHDLAI
                     YCRMLLRGGAPIFKAETVAKMTSVETPANVAVRRAGGFDLDSSFSRPRGEIFPLGSFG
                     HTGFTGGMFWIDPFSKTFFIFLSNRVHPDGKGDVLHLQSDLGTLAAEAARVPKESPAG
                     ALPVRAGGQVRLTYGGADTNNGIDHLEAERYAPLRGMRIGLITNHTGIDRAWNPTIDS
                     LRSAPGVTLVALFSPEHGIRGTADEKIGDSVDAASGLPVYSLYADMRKPKPEQLAGLD
                     ALVFDIQDVGARYYTYIATMGLAMEAAAAAHIKFIVLDRINPIGGTAFEGPLLHGDES
                     FIAWHSLTIRHGMTVGELARMFNDERHIGADLTVIPLQRWRRELWMDEAGLPWINTSP
                     NMRSLTEATLYPGIGILE"
BASE COUNT          560 a         1146 c         1014 g          650 t
ORIGIN      
        1 gccatcatcg tcgcctacct cgggggtgta gtcgcgatcg ggttctggtg ccggaagcgg
       61 cagaagacga cgcgccacta ctttctggca gggcgcgatc ttccgtggtg ggcgatctcc
      121 ggatcgatcg tggccacgga aacttctgcg atcacgttca tctccgtgcc cggtctcatt
      181 tacgcgcgag gcggcaactt cacgtttctg cagctcgtgt tcggctacct gatcggccgg
      241 gtcgtcatct gcctgctctt cattcctgcc tactttcgcc ggaacctgct gacgatctac
      301 gaactgctgc ggagccgttt tggcggcagc gtgcgcagtc tggccgcatc gctcttcatc
      361 atcatgcgca cgattgccga cggggtgcgc cttctcttga cctccgtcgt gatgtcgacc
      421 gtgtggcacg cctttcttcc gaagaccgat atggcgaccg ccgcggccat ctccgtcatc
      481 tgcattggcg gcgtgatgct gctcttcacg accgtgggcg gcatggaggc ggtcatctgg
      541 accgagatcg tgcacgttgg tgtctacatc atcggcgcgg gtgccgcgat cgcgatcctc
      601 gttcacgcaa tccccggcgg gttgtccggc gcaattgcag cggggagcgc ggcggcaaag
      661 ttccgcctct tcgatttctc gctcgacttc acgcggccct acgtattctg ggccggcgtc
      721 atcggcggcg cgttcctcaa catgtcgact catggcacgg atcagtacat ggtgcagcgc
      781 tatctctgca cgaccagcgc gcgcaaagcg caggcggcgc tcctcgtctc cggaatggtc
      841 atcctcgtgc agttcgcagt cttccttttc atcggcgttc tcctctttgg ctactaccat
      901 ccggcatcgc tgcctgggta tgcgacagga cccgcggccg caccgtttat ggctcccgac
      961 caagtcttcc ctgacttcat cgtgcatcac gtacccatcg gactctcggg cttgctcgtc
     1021 gctgcggtcc tggctgctgc gacttcccct tccgtcaact ccatcgctgc aaccgcggtc
     1081 tccgatctct accaaccgct cgttcgcgga cgaagcgatg gtcactacct ccgcgtctcg
     1141 cgcatactca cggtggttgc cggcgtggcc cagatcggag ttgcgctcgc gctgcagggg
     1201 caggcacgct cggccgtcga caccgcgctc ggcgtggcgt cattgatcaa cggaccaatc
     1261 ctgggcgtct ttctactcgg tagcatgcgt cgggccggcc gcatcggcgc gctctgcggc
     1321 atgagcgcgg gcattgccgc cgtcaccgca gtcttcgccg cgactcatat cgcgtggccc
     1381 tggtacaccg tcatcggttc gttgactacg ttcgccgttg gaacgttgat tccggagagg
     1441 gaacatgcgc cgatttagca tcgccgttgc cgcactcctc gccggttgtc tgacgacgca
     1501 actggcggca cgcttcgatc ccgaccggct gcgagcgatc gatcagacca tcgagaccgc
     1561 catcgctgcg cacaaagttc ccggcggcgt ctttcatctc gagcgcgacg gcgcgatcta
     1621 cgagaaggcg tacggcaacc gcgcgctcgt cccggctgtc gagccgatga ccgtcgatac
     1681 gatcttcgac gccgcgtcga tcacgaaggt tgtggccacg acaccgagcg tctggttgct
     1741 gatcgagcgc gggaagatcg agctcgatgc gccggtcagc cgctacatcc ccgcattcaa
     1801 aggcggctgg cgtgacgaga tgacgatccg ccatctgctc acgcacacat ccggccttcg
     1861 ccctgacctc gacctcaccc ccgcgtggag cggctacgac acagccatcc gcatggcgat
     1921 cgccgaggag ccgcgacaga gaccgggata cgtgttccgc tacagcgaca tcaacttcga
     1981 gctcctcggc gagatcgtgc gccgcgtttc cggagagccg ctcgatgtct tcgccaagcg
     2041 cgagatcttc gagccgctca agatgcacga cacgagtttt cgcgcgcgta gcgccgacgg
     2101 ctcgtcggct gttccgccgg cggctcgccg gcaagacacc aaccgcatcg ctccaacgga
     2161 acaaaccccc gagggcatgt tgcgcggcgt cgtgcacgac ccgacctccc gccgcatggg
     2221 cggcgtcgcc ggacacgccg gactcttcac gaccgcgcac gacctcgcca tctattgccg
     2281 catgctgctg cgcggcggtg cgccgatctt caaagccgag accgtcgcga agatgacgag
     2341 cgtcgaaacg ccggcgaacg tcgcggtgcg gcgcgctggc ggattcgatc tcgactcatc
     2401 attcagcagg ccgcgcggcg agatcttccc cctcggatcg ttcggccaca ccggattcac
     2461 cggcggtatg ttctggatcg atccattctc gaaaacgttc ttcatcttcc tttcgaaccg
     2521 cgtccacccg gatggcaaag gcgacgtcct gcatctccag tccgaccttg gcacgctcgc
     2581 cgcggaagct gcgcgcgtac cgaaggagtc gccggctgga gcgttgccgg tgagggcggg
     2641 cggacaggtg cgcctcacgt acggcggcgc cgacacgaac aacggcatcg atcatctcga
     2701 agcagagcga tacgcgccgc tccgtggcat gcgcatcggt ctcatcacga accacacggg
     2761 aatcgatcgg gcatggaacc cgacaatcga ctcgttgcga agcgcgcccg gcgtcacgct
     2821 cgttgcgctc ttctcgcccg agcacggcat acgaggaacg gcggacgaaa agatcggcga
     2881 ctctgttgat gccgccagtg gccttcccgt ctacagcctc tacgccgaca tgcgcaaacc
     2941 gaaaccggag caactggccg gactcgatgc gctcgtcttc gacattcagg atgtcggcgc
     3001 gcgctactac acctacatcg cgacgatggg actggcgatg gaggcggcgg ccgcggcgca
     3061 catcaagttc atcgttctcg accgcatcaa tccgatcggc ggcaccgcct tcgaaggacc
     3121 gctgctgcat ggcgacgaga gtttcatcgc ctggcattca cttaccattc gccacggaat
     3181 gaccgtcggc gagctggcgc ggatgttcaa cgacgagcga cacatcggcg ccgacctgac
     3241 cgtgattcca ttgcagcgat ggcggcgcga gctgtggatg gacgaggccg gacttccgtg
     3301 gatcaacacc tcgccgaaca tgcgcagcct gaccgaagcg acgctctatc ccgggatcgg
     3361 catcctggag
//
LOCUS       DATLVW010000414         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_307780, whole genome shotgun sequence.
ACCESSION   DATLVW010000414 DATLVW010000000
VERSION     DATLVW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307780"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VNN25_12490"
     CDS             <1..328
                     /locus_tag="VNN25_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92391.1"
                     /translation="IVFSAAAFYPALLDMDFWGYLTIVAVSVAALAIGHWLGPSDAHE
                     KTTLAVECGVRHPALAISIAAARLTPEKAMPILVPCVLTFILMAMIYLLLRGRALRAA
                     ESQTVP"
     gene            768..1274
                     /locus_tag="VNN25_12495"
     CDS             768..1274
                     /locus_tag="VNN25_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92392.1"
                     /translation="MAATALLYTLSPVRATGSSLPERTARSNETIGVRTVPALKTAAP
                     TVLLTHLPLVMRVGKDEFRVAFGIKSAGCAATGCHGVVRYRVQWKADDGTTRRDIREV
                     DFAVAPHSADRTITVDRQYFDTAEGAHTTDVVQVTVDRLTCQPDSEGSVLARNASREQ
                     TLPPIRAD"
     gene            1581..>2633
                     /locus_tag="VNN25_12500"
     CDS             1581..>2633
                     /locus_tag="VNN25_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXH92393.1"
                     /translation="MILPENAPFRIGDWRIDPALDEISCDGRTIKLEPRTMCVLVCLA
                     RRAGEVVSVDDLLDAVWKDVVVTQYSVYQAVAALRRSFGDDPKAPTYIDNVPRRGYRL
                     LAPVAVPATSPPAEAAAEPALAERSTTVVIPAHAQSGTVGIIRAPVPASWPLKVGVLV
                     AVALVIFGTAAAYLAIGKLRISKSAVSSPTVAASPTPTAFSPPAHSIAVLPFVNMSGD
                     KEQEYFSDGLTEELLNSLAEIDGLQVAARTSAFSFKGKDADIGTIARKLNVGAVLEGS
                     VRRSAHTIRITVQLINAVTGFHLWSKTYDRDLGDVLRLQTEIATSVASALKVTLLGDV
                     AAKVELGGTRNPAAFDA"
BASE COUNT          468 a          835 c          846 g          484 t
ORIGIN      
        1 gatagtgttc tcggcggcgg ctttctatcc cgcgttgctg gacatggatt tttggggcta
       61 cctgaccatc gttgccgtga gcgtcgcggc gctggccatc ggccactggc taggtcccag
      121 cgatgcccac gagaagacga cgctggcggt ggaatgcggg gtccgccatc cggcattggc
      181 catctcgatc gctgccgcgc gcctcactcc cgagaaggca atgccgatac tggttccgtg
      241 cgtgctgact ttcatcctca tggcgatgat ctatctgctg ttgcggggca gagcgctgcg
      301 cgctgccgag tcgcaaaccg tgccctgacg agcggtttcg ccggccagtg cgcgacgctg
      361 ctgtcgatgt gttgaaaccg gacccgatcc ggaatgccgc gccgccatcg agagtcacgt
      421 gtgctctcta agtcgctgat gcaaagtagg tttttcacgg catagcggtg gcgctggcct
      481 cagagccaag cggccccagc ccgggacgtt acgcccgtgc tacacgcctt ccaccttcct
      541 tcaacgatcc tttaagtatt gatgaacgtc tcctcaggac ttccggagcg tgccccggca
      601 ttgttgcggc cggttggcgg agatccccct ggcctcagcc ggggaacacg cactcagttt
      661 gaacctaaca ggagatggaa atggaacaga tcatgacgtc gagtggcttc tctcgggtca
      721 ctgtgccctt tcgagtggct cgaggaggtt ggagagttcc ctgtgtcgtg gctgcaacag
      781 cgctgctcta cacgctgagt ccagtccggg ctaccggaag ctcattgccc gaacggacgg
      841 cgcgttcgaa cgaaactatc ggggttcgca cggttcctgc cctgaaaacc gccgcgccaa
      901 cggtgttact cacccacctc ccgctggtga tgcgcgtggg caaggacgaa ttccgggttg
      961 ccttcggcat caaaagcgcc ggttgcgccg ctactggctg ccacggggtg gttcgatacc
     1021 gcgtgcaatg gaaagccgac gatggcacga cccgccggga catcagggag gtcgactttg
     1081 cggtggcgcc tcacagcgcc gaccgaacga tcacagtcga ccggcagtac ttcgacacgg
     1141 ccgaaggcgc gcacacgacc gatgtcgtgc aggtgacggt agaccgtctc acctgccaac
     1201 cggacagcga aggcagcgtg ctggcgcgga atgcgagccg ggagcagaca ctaccgccca
     1261 ttcgagccga ctaaacgagg cacaaagcca gcgatgccac cgaagcggac gtgccggctg
     1321 cgatggcggc ggagtgttgc cgtctgcgcg cgtcgacggt cgggcagtac gggctcacaa
     1381 tctgggactt cacaatcgat ctgagggggg cctaaggaga cccgcccctc gataggggac
     1441 ggtggatgct cctgggtgac gcgcagccgc gccagccagg gctgagcgtg cagacgtggg
     1501 agggccgccg gcgcgcgtgt cgtgccggta tactaataag cgacatcgcc ctcttattcc
     1561 tttgagtcga tacatcgccg atgatcctgc cggaaaacgc gccctttcga atcggcgact
     1621 ggcggataga cccagcgctg gacgaaatct cgtgcgacgg caggaccatc aagctggaac
     1681 cgcgcaccat gtgcgtcctg gtctgcctcg cccgccgcgc cggggaggtg gtgagcgtcg
     1741 acgaccttct cgatgccgtc tggaaggacg tcgtcgttac ccagtattcc gtatatcagg
     1801 cagtggcagc gctccgccgc tcgttcggcg acgaccccaa ggcaccgacg tacatcgaca
     1861 acgtgccgcg ccgcggctac cgcctccttg cgcccgtggc tgtgccggca acctcgccac
     1921 ctgcggaagc ggctgcggag cctgctctcg cggagcgatc gactacggtc gtgattcccg
     1981 cgcatgctca gtctgggacc gtagggatca tccgagcgcc tgtgccggcg tcttggccgc
     2041 taaaggtagg cgtgctcgta gcagtggcgc tggtgatctt cgggacggcg gcggcctatc
     2101 tcgccatcgg gaagctccgg atctcgaagt ccgccgtgtc gtcaccgacg gttgcggcca
     2161 gcccgacgcc tactgccttt agcccgccgg cgcactcgat cgcggtgctc ccattcgtca
     2221 acatgagcgg ggacaaggag caggagtact tttctgacgg actcacggag gaactcctca
     2281 actccctggc cgagatcgat ggactgcagg tcgcggcccg cacatcggcg ttttcgttta
     2341 aggggaaaga tgccgatatc ggcacgatcg cgcgcaaact gaacgtgggc gcagtcctcg
     2401 aagggagcgt gcggcgctcc gcgcacacca tccgcatcac cgtgcagctg atcaatgccg
     2461 tcacgggctt tcacctgtgg tcgaagacct atgaccggga cttaggggat gtgctgagac
     2521 ttcagaccga gattgccacc tctgtggcca gcgccctgaa agtcacgcta ctcggcgatg
     2581 tcgcggccaa agtcgagctc ggcgggacgc gtaaccccgc cgccttcgat gcg
//
LOCUS       DATLVW010000415         2944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_307793, whole genome shotgun sequence.
ACCESSION   DATLVW010000415 DATLVW010000000
VERSION     DATLVW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307793"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VNN25_12505"
     CDS             <1..868
                     /locus_tag="VNN25_12505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92394.1"
                     /translation="SYDLLHLHSVFLWPTYTAARAAERARIPYVVSPRGMFVTELIRR
                     KSPLVKRAWIRLIERHTFRHASAIHFTAQREWDDARLISILLPAPFIVPNGIDLPPPS
                     PPARLDDTLLILGRINWKKGLDRLLDAIKLVPTANVIIAGNDEEELLPKLRAQAERNG
                     IAGRVDFRGPVSGEAKDQLLRTSTALVLPSLSENFGNVVLEAMAVEMPVIVTPEVGLA
                     ADVAHSGAGIVSSGQPVELAAAIQHLLRDRDARADMGQRGRELVQSQFTWERVAAQME
                     EHYLQIAAVAGP"
     gene            914..1672
                     /locus_tag="VNN25_12510"
     CDS             914..1672
                     /locus_tag="VNN25_12510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH92395.1"
                     /translation="MLERVTPLILTRDEEANIGRTLEQLAWAREVVVVDSESTDATAA
                     IAGRFPNVRVVRRAFDSHAAQWSFGVAQVSTDWVLTLDADYFVPERFVQEIASLDPPP
                     DVDGYAAAFVYAVHGKPLRATLYTPRPVLLRRGAFEIWQDGHTQRVRVQGRTERLRTR
                     LIHDDRKDLRRFLERQRAYMRKESQKLRATPWRKLPLSGRIRKLRVVSPFATLAYVLF
                     VKRTILDGAAGLRYALERFLAEGILSIELFRKRG"
     gene            complement(1715..2374)
                     /locus_tag="VNN25_12515"
     CDS             complement(1715..2374)
                     /locus_tag="VNN25_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HXH92396.1"
                     /translation="MENVVSSLDQEVQQRHLLNHPFYQRWSAGELSRPELEEYARQYY
                     HYALAFPTFLSGMHAHTEDMVIRQHLLENLIEEERGPENHPELWLRFCEALSLDRTDV
                     VSGTPNAATRALIDSMRTFAHSVSLHEGLAALYAYESQIPAVAKAKIDGLAAWYGITE
                     PRDVAFFSVHMTADVEHAGTARMLLDQLCDTPEKSEQARLSAARTLGRLYDFLDAVTV
                     N"
     gene            complement(2460..>2944)
                     /locus_tag="VNN25_12520"
     CDS             complement(2460..>2944)
                     /locus_tag="VNN25_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92397.1"
                     /translation="KYRKTFGLPERLSPVEEREGALGPWYANTLNAGSQRMLHYMSGP
                     SLLSVIIPLRDRRSAEQRFVAALRELLGALDVPERYVDLEAGCMGVIQHGRASDRSKL
                     GSLRDQAYTASYDFYDGLSLALINRRAAMTPCGPMAYKSPEYVAPMLLEQTWRLTAAN
                     "
BASE COUNT          540 a          964 c          898 g          542 t
ORIGIN      
        1 ttcctacgat cttctgcatc tgcactccgt tttcctctgg ccgacgtata cggcagccag
       61 ggccgcggag agagcgcgca tcccgtacgt cgtctccccg cgcggcatgt tcgtcacgga
      121 actgattcgc cggaagagcc ctctcgtcaa gcgcgcgtgg atccggctca tcgagaggca
      181 caccttccgg cacgcatccg ccatccattt caccgcgcaa cgggagtggg atgacgctcg
      241 cttgatttcg atcctgcttc ctgcgccgtt catcgtgccg aatggaatcg accttcctcc
      301 accatcgcct cccgcgcgtc tcgacgacac tctcctgatc ctcggacgga tcaactggaa
      361 aaagggcctt gaccggcttc tcgatgccat caagctcgtt cccacggcga acgtgatcat
      421 cgccggaaac gacgaggagg agctcctgcc gaagcttcgg gcgcaggcgg aacggaacgg
      481 catcgccgga cgcgtcgact ttcgcggacc ggtgagcggc gaggctaagg accagttgct
      541 gcgaacatcg acagctctgg tgctgccttc cctttccgag aacttcggca atgttgtcct
      601 tgaagcgatg gccgtcgaga tgccggtgat cgtcacaccg gaagtgggct tggccgccga
      661 cgtcgcgcac agcggcgccg ggatcgtttc gtccggccaa cctgtggagc tggcagcggc
      721 gattcaacac ctgctgcgcg accgcgacgc gcgcgcggac atgggccagc gagggcgcga
      781 gctggtccaa tcgcagttca catgggagcg cgttgccgcg caaatggaag agcattactt
      841 gcagatcgcc gcggtcgccg ggccgtaacg cgagagacgc aggcgaaaac cgtccgctgc
      901 taccatgctc gtgatgctcg aacgcgtcac gcctctgatc ctcacacgcg acgaggaggc
      961 caacatcggg cgcacgctgg agcaactcgc atgggcgcgc gaggtggtcg tggtcgacag
     1021 cgaaagcacc gatgccacgg ccgcaatcgc cgggcggttc ccgaacgtgc gcgtcgtgcg
     1081 gcgcgcgttc gactcccatg cggcgcaatg gtcgttcggc gtagcccagg tctccaccga
     1141 ctgggtgctc actctggacg cggactactt cgtccccgag agattcgtgc aggagatcgc
     1201 ttctctcgat ccacctccgg acgtcgatgg ctatgcggcg gcattcgtct atgcggtcca
     1261 tggcaagcca ctccgggcca cgctctacac gcctcggccg gtactactgc ggcgcggcgc
     1321 attcgaaatc tggcaggacg gccatacgca gcgcgtccgc gtgcagggcc gcaccgagcg
     1381 cctgcgtacg cgtctgattc acgatgatcg caaagatctg cgccgcttcc tcgagcgcca
     1441 acgcgcctac atgcggaagg agtcgcagaa attacgggca acgccctggc gcaagctgcc
     1501 tctttccggc cgcatccgca agctacgagt cgtttcgccc ttcgccactc tggcgtacgt
     1561 gctcttcgtg aaacggacga ttctcgacgg cgccgccggt ttgcgctacg cgttggagcg
     1621 attccttgcc gagggcattc tctcgatcga gttgttcaga aagcgaggat gactcgcgta
     1681 gcctcgggac agagaccacg cagggttcct gatgtcagtt cacggtcacc gcgtcgagga
     1741 agtcgtacaa ccgccccaat gtccgcgctg ccgacaaacg cgcctgctcg cttttctccg
     1801 gtgtgtcgca taactgatcg aggagcatcc gcgccgtacc ggcgtgctcc acatcggcgg
     1861 tcatgtgcac ggagaagaac gccacgtcgc gcggctcggt gatgccgtac cacgccgcga
     1921 ggccgtcgat cttcgctttc gcgaccgcgg ggatctgcga ctcgtatgcg tagagcgcgg
     1981 ccagaccttc gtgcagtgac acgctgtggg cgaaggtccg catcgagtcg atcagcgcgc
     2041 gcgtcgccgc attcggcgtt cccgacacca cgtctgtacg atcgagcgac agcgcttcgc
     2101 agaaccgcag ccagagctcg ggatggttct ccgggccgcg ctcctcctcg atcagattct
     2161 cgagaagatg ctggcggatg accatgtctt cggtgtgcgc atgcatgccg ctcaggaagg
     2221 tcggaaaggc gagagcgtag tggtagtact ggcgcgcgta ttcttcgagc tcggggcgcg
     2281 acaactctcc cgcgctccag cgctgataaa acgggtgatt gagcagatgg cgctgctgca
     2341 cttcctgatc gagagagctg accacgttct ccatccaatc ctccgcaagc tgcgatttca
     2401 ggagacggta tcatgcacac ggcatcatca actccgccca gcacgagcag caaaggagct
     2461 tagttggcag ccgtcaatct ccacgtttgc tcgagcagca tcggggcgac gtactccggc
     2521 gatttgtacg ccatcggtcc acacggcgtc atggccgccc ggcggttgat cagcgcgaga
     2581 ctgagaccat cgtagaaatc atagctggcc gtgtacgcct gatcacgcag agaaccgagc
     2641 ttgctccggt cgcttgcacg gccgtgctga atcactccca tacaacctgc ttcgagatcc
     2701 acgtaccgct caggaacatc gagagcgccg agaagttcgc gcaacgctgc cacgaatcgc
     2761 tgttctgcgg agcgacgatc ccgaagcggg atgatcacgg acaacagcga cgggccgctc
     2821 atgtagtgga gcatcctctg actcccggcg ttgagcgtgt tcgcgtacca cggccccagc
     2881 gcaccctcac gctcctcgac gggcgacaac cgctccggaa ggccgaacgt ctttcgatat
     2941 ttcg
//
LOCUS       DATLVW010000416         5370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_62030, whole genome shotgun sequence.
ACCESSION   DATLVW010000416 DATLVW010000000
VERSION     DATLVW010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62030"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VNN25_12525"
     CDS             <1..684
                     /locus_tag="VNN25_12525"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXH92398.1"
                     /translation="VDGSDGLYNGAYDNASGIASLIEIARALASGPRPKRSVLFLALT
                     GEEKGEQGSEYFVHFPPVPVRQVVADVNMDMFLMIYPVADLVPLGGEHTSIGAMAAAA
                     AHASGFDVSPDPYPEEVRFIRSDQYSFVKAGIPAIHLKPGNKSSEPGIDGAKMTRDWL
                     RNIYHSPRDDMSQRFDFASGARYAETNLRLVRAIANASQRPAWTDGDFFGETFGRGGR
                     KATITLRRN"
     gene            1023..1796
                     /locus_tag="VNN25_12530"
     CDS             1023..1796
                     /locus_tag="VNN25_12530"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXH92399.1"
                     /translation="MTAHDTGVAPRVEDVLGRSFEFTAPPRRMVSLVPSLTETLFDLG
                     AGDDVAGVTDFCIFPENLNKPRVGGTKNPRIDEIRALAPDLVYVNLEENLERHAQAIG
                     GFAPVFVTEPKTVDDVAELIATLGVIHDRNDRARELNDELGQERGHVASFTFAVPIWK
                     KPWMWCGGDTYVSNLVESTGGRNVLHDEMRYPSLSLEQVLALRPDVVFLPDEPYVFTS
                     DDAAEITGTRVIGPFPGHLFTWHGTRTILGLRFLRECFS"
     gene            complement(1776..2588)
                     /locus_tag="VNN25_12535"
     CDS             complement(1776..2588)
                     /locus_tag="VNN25_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH92400.1"
                     /translation="MSEVPITKRPVVYRMAGQGVVIVRRDVPYRDDLTIDLYYPPDSK
                     AEIRRPAVIFVTGYPDPGFQKFLGCRQKEMASYISWAELAAASGLIAVTYSNREPPAD
                     AEAVLAFIRQNAETLGIDETRIGIWSCSGSVPTALSVLMNRPSDAFRCAVLCYGLMLD
                     LDGYTAVEDGAKQFGYRNPTSGKLVSDLPPTLPLMIVRAGRDEFPHLNETLDRFVSKA
                     LECNLPVTLINHADAPHAFDTAHDSEMSREVIRHVLAFMRFHLIDESREALA"
     gene            complement(2596..3231)
                     /locus_tag="VNN25_12540"
     CDS             complement(2596..3231)
                     /locus_tag="VNN25_12540"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH92401.1"
                     /translation="MNPKPLRHIDLFSSAGKLEALYRDLQDPAAVGVVCHPHPLGGGT
                     LHNKVVFRAARGLEAANVATLRFNFRGTGASAGHHEDGEGEQADVVAAIDWLTKKHPD
                     AKIIVGGFSFGAWVASRVACEISTVDALFLIGTPVNKYDFGYLHSCEKPMLILHGTQD
                     EFGDVEKVEKLGQQIRNAETMIITGADHFFTRQLDSVEETMRLWATEIIAG"
     gene            complement(3228..3566)
                     /locus_tag="VNN25_12545"
     CDS             complement(3228..3566)
                     /locus_tag="VNN25_12545"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXH92402.1"
                     /translation="MTEAFIEVLRETDLPVGRIKSVKIDDHTVAICHTSRGFFATDNT
                     CPHRGGPLSEGDLIGDEIVCPWHLWGFDVTTGRCPGNRDFTIATHDVKVDSGRILVKL
                     SPVRETSDAL"
     gene            complement(3614..4093)
                     /locus_tag="VNN25_12550"
     CDS             complement(3614..4093)
                     /locus_tag="VNN25_12550"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HXH92403.1"
                     /translation="MKQIAAILSATLVALTLAAATTPPNLGWLKFETKVSDKVLKWKV
                     RNETPPAVAPAQFPNRIEMHWKYTPDAAGMPSPEVEANFARLEAATDPIQGDHVAYLM
                     MVVTGNGDRTWFWYAGDAKAFAAELNNLLPGHPYPVTLHLAGNEPDWKTYQTMRGKR"
     gene            complement(4090..5232)
                     /gene="amrS"
                     /locus_tag="VNN25_12555"
     CDS             complement(4090..5232)
                     /gene="amrS"
                     /locus_tag="VNN25_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXH92404.1"
                     /translation="MPAVTEGQRVLGEILAKRTTEGAPELYDKLENAAVRCYSCGHRC
                     KILEGLNGICKVRYNEGGVLRVPRGYAAALQVDPIEKKPFFHAYPGALALSFGMLGCD
                     YHCGYCQNWVTSQALRDPVAGAAPTDIEPSELVKLAKQHGAKVITSTYNEPLITSEWA
                     VEIFKEAKREGLVCSYVSNGNGTERVLDYIAPWVDLYKVDLKGFSDAHYRDLGGTLQP
                     VLDTIRGLKQRGIWLEVVTLLVPGFNDSDEEMTKMAEFLASVDVDIPWHITAFHQDYK
                     MTGNANTTARQLLRGYEIGRRAGLRFIYPGNIPGAVGDLESTHCPSCDRVVIGRLGFR
                     VISYHLVDGHCPDCGTAIPGVWDGNPAVGRSGIPRPVHLSREELSE"
BASE COUNT         1013 a         1636 c         1692 g         1029 t
ORIGIN      
        1 gtcgatggca gcgacggtct ttacaacggc gcctacgaca acgcctcggg tatcgcatcg
       61 ctgatcgaga ttgcacgcgc tctggcatcc gggccgcgtc cgaaacgatc ggtcctcttc
      121 ctcgccctga ccggcgaaga gaagggcgag caaggttccg agtacttcgt ccacttcccg
      181 cctgtgccgg tcaggcaggt ggttgccgac gtgaacatgg acatgtttct gatgatctat
      241 ccagtcgccg atctcgtccc gctcggcggc gagcacacgt cgataggcgc gatggccgcg
      301 gccgccgcgc acgccagcgg cttcgacgtc agtcccgatc cgtatcccga agaagtgcgc
      361 ttcattcgca gcgatcagta ttcgttcgta aaggcgggca tcccggcgat ccatctcaaa
      421 ccggggaaca agagcagcga accggggatc gacggcgcga agatgacacg cgactggctg
      481 cgcaatatct atcactcgcc gcgcgacgac atgtcgcaac ggttcgactt cgcgtcggga
      541 gcgcgttacg cggagacgaa tctgcggctg gtgcgggcga ttgccaatgc atcgcagcgg
      601 ccggcgtgga cggatggcga cttctttggc gagacgttcg ggcgcggcgg gcggaaggcg
      661 acgatcacgc tgcggaggaa ttgaggaact cttctcatct cgccgagggc gacactccgc
      721 tgtcattctg agaaggcccc ggatgtgtca tcctgaggct gccggaggcg cccgcgctgc
      781 tcgatcccct cgcgctaaac ccgtccggcg ccgcaggacg ccgaaggatc tcaagttgcc
      841 gcgcgcatgg catgtgcttc gtagtttgag atccttcggc gtcctgcgcc gccacgtctt
      901 gcagcgtgaa cacaacgagt acgaatggcg gctccggcag cctcaggatg acacatcaat
      961 ctccattgcg aagttgacaa accatcggtt tcacgggcgt atgcattgac ccacctctcg
     1021 gaatgacagc gcatgacaca ggggttgcgc cgcgcgtgga ggatgttctc gggcgcagct
     1081 tcgaatttac cgctccgcct cggcgcatgg tctcgctcgt tccatcgctt accgagactc
     1141 tcttcgacct cggcgcgggc gacgatgtcg cgggcgtcac ggacttctgc atcttccccg
     1201 agaacctgaa caaaccgcgg gttggcggca cgaagaatcc gcgcatcgat gagattcgtg
     1261 cgcttgcgcc ggacctggtc tacgtcaacc tcgaggagaa cctggagcgg catgcacaag
     1321 cgatcggggg cttcgcgcca gtgttcgtga ccgagccgaa gacggtcgat gatgtcgcgg
     1381 agttgatcgc aaccctcggc gtgattcacg accgcaacga tcgagcacgt gagctgaacg
     1441 atgagcttgg gcaggaacgc ggacacgtcg cgtcgttcac cttcgccgtc ccgatatgga
     1501 agaagccgtg gatgtggtgc ggcggcgaca cgtacgtatc gaacctcgtc gaatcgaccg
     1561 gcggccgcaa cgtgctgcat gacgagatgc gctacccgtc gctatcgctc gaacaggtgc
     1621 tggcgctccg tcccgacgtc gtcttccttc ctgacgagcc ctacgtgttc acgtccgatg
     1681 atgctgccga gatcacaggc acacgtgtga tcggcccgtt ccccggccac ctcttcacgt
     1741 ggcacggaac gaggactatc ctcgggctgc gatttctacg cgagtgcttc tcgtgattcg
     1801 tcgatgagat gaaatcgcat gaaggcgagc acgtgccgga taacctcgcg cgacatctcg
     1861 ctgtcatgcg cagtgtcgaa cgcgtgcggc gcgtcggcgt gattgatgag cgtaaccggc
     1921 agattgcact cgagtgcctt gctcacgaag cgatcgagcg tttcgttcag gtggggaaac
     1981 tcgtcgcggc ccgcacggac gatcatcagc ggaagcgtcg gcggaagatc gctcactaat
     2041 ttgcccgacg taggattcct gtagccgaac tgctttgcgc catcctcaac ggcggtgtag
     2101 ccatcgaggt cgagcatcaa tccgtagcag agaactgcgc agcggaatgc atccgaaggc
     2161 cggttcatca gcaccgacaa cgccgttgga acgctgcccg agcatgacca gatgccgatc
     2221 cgcgtttcgt cgatgccgag cgtttccgcg ttctggcgga tgaatgcgag caccgcttcg
     2281 gcatcggccg gcggctcgcg attcgaatag gtgacggcga tgagtcccga cgccgcggca
     2341 agctccgccc aggaaatgta ggaggccatc tccttctgcc tgcatccgag gaacttctgg
     2401 aatccgggat cgggatagcc ggtcacgaag atcaccgcgg gcctgcggat ctcggccttc
     2461 gaatccggcg gatagtaaag atcgatcgtg aggtcatcgc gatacggaac atcgcgccgc
     2521 acgatcacga cgccctgacc ggccattcga tagacgaccg gccgtttcgt gatgggaact
     2581 tcgctcatca acaaactacc ccgcgatgat ctcggtcgcc cagaggcgca tcgtctcttc
     2641 gacggagtcg agctggcggg tgaagaagtg atcggctccg gtgatgatca tcgtttcggc
     2701 gttgcgaatc tgctggccga gcttctcgac cttttcgacg tcgccgaatt cgtcctgcgt
     2761 gccgtgaagg atcagcatcg gcttctcgca cgagtgcagg tagccgaagt cgtacttgtt
     2821 gacgggcgtt ccgatgagaa aaagagcatc gacagtgctg atctcgcacg cgacacgcga
     2881 ggccacccag gcgccgaagg aaaagccgcc gacgatgatc ttcgcgtccg gatgtttctt
     2941 cgtcagccag tcgatcgcag cgacgacgtc cgcctgctcg ccttcgccgt cttcgtgatg
     3001 tccggcgctg gcgcccgtgc cgcggaagtt gaaacggagc gtagccacat tcgccgcctc
     3061 cagtcctcgg gcggcgcgga aaacgacttt gttgtgcagc gttcctccgc cgagggggtg
     3121 gggatggcag acgacgccga cggcggcggg atcctgaaga tcgcggtaaa gcgcttcgag
     3181 cttcccggcg ctcgagaaga gatcgatgtg gcggagcggc ttcggattca cagtgcgtcc
     3241 gatgtttcgc gcaccggcga aagcttgacg aggatgcggc cgctgtcgac cttcacgtca
     3301 tgcgtggcga tcgtgaaatc cctgttcccc gggcaccggc cggtcgtgac gtcgaatccc
     3361 cagaggtgcc acggacagac gatctcgtcg ccgatcaagt cgccctcgga cagcggtccc
     3421 ccgcggtgag gacaggtgtt gtcagtcgca aagaatccgc gcgatgtgtg gcagatcgcg
     3481 accgtgtggt catcgatctt cacggacttg atccgcccga ccggcaggtc agtctcgcgg
     3541 agaacctcga taaatgcttc ggtcatcgga cgcgcactct aacaaaccgc tcgcgattcg
     3601 gttcgccgca ggctcagcgc tttcccctca tcgtctggta agtcttccag tcgggctcgt
     3661 tcccggcgag gtgaagcgtg accggatacg ggtgtccggg cagcagattg ttgagctcag
     3721 cggcgaacgc tttcgcatcc ccggcatacc agaaccaggt tcgatcgccg ttgccggtga
     3781 cgaccatcat caggtaggcg acgtgatcgc cctgaatcgg atcggtcgcc gcttcgagcc
     3841 gggcgaagtt cgcctcgact tccggtgaag gcattcccgc cgcatccggc gtgtacttcc
     3901 aatgcatctc gatccggttc ggaaactgcg caggcgcaac cgcgggcgga gtctcgttcc
     3961 gcaccttcca cttcaaaacc ttgtccgaca ctttcgtctc gaacttgagc catccgaggt
     4021 tgggcggagt ggtggcggcg gcaagcgtta atgcgacgag tgtcgcgctc agaatggcag
     4081 cgatctgttt cattcggaca gttcctctcg cgaaagatgc acggggcggg gaatgccgct
     4141 gcgtccgacc gcgggattgc catcccacac acccgggatg gctgtaccac aatcgggaca
     4201 gtgaccgtcg accagatgat acgagatcac gcggaatccg agacggccga tgacgacgcg
     4261 atcgcacgac ggacaatgcg tgctttcgag atcacccacc gctcccggga tgttgcccgg
     4321 ataaatgaac cgcagtccgg cgcgccggcc gatctcatat ccgcgaagaa gctggcgcgc
     4381 cgtcgtattg gcgttgccgg tcatcttgta gtcctgatga aacgcggtga tgtgccacgg
     4441 aatgtcgacg tcgacgctcg cgagaaactc ggccatcttc gtcatctctt catcggaatc
     4501 gttgaagccg ggcacgagga gggtgacgac ttcgagccag atgccgcgct gtttgagacc
     4561 gcgaatcgtg tcgagcaccg gctgcaaggt gccgccgagg tcgcgatagt gcgcgtcgga
     4621 aaatcctttg agatcaacct tgtagagatc gacccacggc gcgatgtaat cgagcactct
     4681 ctccgtgccg ttgccgttgg aaacgtacga gcagacgagc ccctcgcgct ttgcttcctt
     4741 gaagatctcg acggcccatt ctgaggtgat gagcggctcg ttgtaggtgg aggtgatcac
     4801 tttcgcgccg tgttgtttgg cgagcttcac gagctcggac ggctcgatgt cggtcggcgc
     4861 cgctcccgcg acaggatcgc gcagagcctg cgaggtcacc cagttctggc agtagccgca
     4921 gtgatagtcg cagccgagca tgccgaacga aagtgccagc gcgcccggat aggcgtggaa
     4981 gaaaggcttc ttctcgatgg gatcgacctg cagcgcggcg gcatatccgc gcggaacgcg
     5041 aagcacgcct ccctcgttgt agcgaacctt acagatgccg ttgaggcctt cgaggatctt
     5101 gcatcgatgg ccgcaggagt agcagcgcac ggcggcgttc tcgagcttgt cgtaaagctc
     5161 cggcgctcct tcagtcgtgc gcttggcgag gatctcgccg agtacgcgct gtccttcggt
     5221 aactgctggc atgcgaagga aaacacgcga ggtcaggcac gtattgccgg aatccggcga
     5281 gctgctgcgt agcacagcca cgccagcaga gcacctgcga cgccatcgat gaggtagtgc
     5341 cagccggtca cgattgagcc gatgaagatg
//
LOCUS       DATLVW010000417         3622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_100169, whole genome shotgun sequence.
ACCESSION   DATLVW010000417 DATLVW010000000
VERSION     DATLVW010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100169"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..409)
                     /locus_tag="VNN25_12560"
     CDS             complement(35..409)
                     /locus_tag="VNN25_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXH92405.1"
                     /translation="MEIIATDAAPKAIGPYSQAIKSGKILYTSGQIALDPGTGNLVEG
                     DFAAQAHRVFENLKAVLAAAGANFSSVAKATVYLTDLANFATLNEIYASYFGATKPAR
                     TTVGVAQLPKGGLVEIDLIAIL"
     gene            488..2608
                     /locus_tag="VNN25_12565"
     CDS             488..2608
                     /locus_tag="VNN25_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92406.1"
                     /translation="MATYLGNSSLSSAVKDRVLSTFQQAVTLYKQGRIDEVVQGCGLI
                     LRMDPMFDPAKKLMEKAKNPSAPIDVDALIPSESGSDALREARAALAARDFQQASNLT
                     AELLRNDFTNEEAREINDTAREKLEAAPFVDQFVRKAEAFARQGNTSAARMELEKARA
                     LDSDHPDIKPVLDSLTASVSTPAASFSSGPSPSFVVDAPPPAPAARGAAQATDFGFTF
                     EEEKASGPDLSFDAFSFDAPTPTAPVAPAATPEESSSFGSGGFSFDTPSAGPGEFDFS
                     TAPVASGDDQRKVQQYLADGDRAEAAGDYQQAIDLWSRIFLIDVTNDEASQRIENAKA
                     KRRDLESKVEALISAGETAFGRDNETARAKFTEALRLDPANLTAHDYLEKLSGSVSGG
                     VTGGFEAPVAPPPSPERSIFDDEIAMSGSYDSLKPPDPGIAKKPVKPKAEVSQTSAKR
                     GSSMGVIATIAAVVIAVALGWFVWSKYMAKPAYDPTLTEATMHQAQVLAKRGHYDEAI
                     AKLQDVKPDDPQHDKALEMIADLQHKKAQSAEMVNGRPAAQVYDESIAAGRAAYEAHD
                     FDTAKKAFDTAARIKPLPPDVKPIYDAASEQSARLEGARALFADQKYQETIATLNPLL
                     QADPQNQSIRRMLVGAHFNLGAAALQEERVPDAIREFDEVLKNDPNDTMAKRSKELAE
                     RYSGQPKDLLYKIYVKYLPLRNVT"
     gene            2660..2989
                     /locus_tag="VNN25_12570"
     CDS             2660..2989
                     /locus_tag="VNN25_12570"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXH92407.1"
                     /translation="MRFDEIELQSVTLDADSPTTAPPSPSSISSFFRRVAALLIDLSL
                     FAALGMALSPLLPSSMSVISIASLAGFLIIVAFYYFAGTRALWGRTIGGAIFDVRAAR
                     TRRTAQR"
     gene            complement(3362..>3622)
                     /locus_tag="VNN25_12575"
     CDS             complement(3362..>3622)
                     /locus_tag="VNN25_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HXH92408.1"
                     /translation="VDEASKHFDDTMRMQLTPPGLTDLKNKLLTTYGKLQSITEVRQR
                     VQRPYRIIDVIAVCDRSPLAFRVAFDPQNRIASVHISPNTSQ"
BASE COUNT          713 a         1144 c         1116 g          649 t
ORIGIN      
        1 tgctccgact cccgaccccc gactcccgac tccctcacag aatcgcgatc aaatcgatct
       61 cgaccaatcc gcctttcggc agttgggcaa cgccgacagt cgtgcgggcc ggtttcgtgg
      121 cgccgaagta cgacgcgtag atctcgttca gcgtggcgaa gttcgcaagg tccgtcaggt
      181 agacggtggc cttggcgacg ctgctgaagt tggctccggc ggcggcgagc accgctttca
      241 ggttctcgaa tacgcggtgt gcctgcgcgg cgaaatcgcc ttcgacgaga ttgccggttc
      301 caggatcgag ggcaatctgc cccgacgtgt agaggatttt gccgctcttg atggcctgcg
      361 aatacgggcc aatcgccttt ggtgctgcgt ctgttgcgat gatctccatg gttgctcctt
      421 ctcgaacgtc ttgccggtag ggcgtgccag tataatcgcg gcacttttca cggagcacac
      481 acggcgcatg gcaacgtacc tcggcaattc atcgctctct tccgcggtga aggatcgcgt
      541 tctctcgaca ttccagcaag ccgtcaccct ctacaaacag ggacgcatcg acgaggtcgt
      601 gcagggctgc ggcctgattc tgcggatgga tccgatgttc gatccggcaa agaagctgat
      661 ggagaaagcg aagaatccgt ccgcaccgat cgacgtcgat gcgctcattc cttccgaaag
      721 cgggtcggat gctctgcgcg aggcgcgcgc cgcgctcgcc gctcgtgact tccaacaggc
      781 cagcaatctc accgccgagc tgctgcgcaa cgacttcacc aacgaagaag cgcgcgagat
      841 caacgatacg gcgcgcgaga agctggaagc ggcgccgttc gtcgaccagt tcgttcgcaa
      901 ggccgaggcg ttcgcgcgcc agggaaatac gtccgccgcg cgcatggagc tagagaaggc
      961 gcgcgccctc gacagcgatc accccgatat caagccggtg ctggactcgc tgacagcgag
     1021 cgtgtcgact cccgccgcat cgttcagttc cggtcccagt ccctcgttcg tcgtcgatgc
     1081 accaccgccg gcaccggcag cacgcggtgc cgcacaagcg accgacttcg gtttcacgtt
     1141 cgaggaggag aaggccagcg gacccgatct ctccttcgac gccttctcgt tcgacgcgcc
     1201 tacgccgaca gctcccgtcg ctccagccgc tacaccggag gaatcatcgt cgtttggaag
     1261 cggtgggttc tcattcgaca caccttccgc gggacccggc gagttcgatt tctctacggc
     1321 acccgtggca tcgggagacg accagcgaaa ggtccagcaa tacctcgccg acggcgaccg
     1381 cgccgaagcg gctggcgatt accaacaggc catcgatctc tggtcgcgca tttttctcat
     1441 cgacgtcacc aacgacgaag cgtcgcagcg gatcgagaac gcgaaggcga agcggcgcga
     1501 tctcgagagc aaagtggaag cgctcatctc cgcgggtgag acggcgttcg gacgtgacaa
     1561 cgagaccgcg cgcgccaagt tcacggaagc gcttcgcctc gatcctgcca acctcacggc
     1621 acacgactat ctggagaagc tgtccggcag cgtctccggc ggagtcaccg ggggcttcga
     1681 ggcgccggtg gctcccccgc cgtcgcccga gcgcagcatt ttcgacgacg aaatcgcgat
     1741 gagcggttcc tacgattcgc tcaaaccgcc agacccgggc attgccaaga agccggtcaa
     1801 accgaaagcg gaggtctctc aaacttccgc aaagcgcggc tcatcgatgg gcgtgatcgc
     1861 caccatcgcc gccgtcgtga tcgccgtcgc cctcgggtgg ttcgtctggt cgaagtacat
     1921 ggccaaaccg gcctacgatc ccacgctcac cgaggcgacg atgcatcagg cgcaggtact
     1981 ggccaaacgc ggtcactacg atgaggccat cgccaagctc caggatgtga agccggacga
     2041 tccgcagcac gacaaggcac tggagatgat cgccgacctg cagcacaaaa aagcgcagtc
     2101 cgcggaaatg gtcaacggcc gccccgccgc gcaggtgtac gacgagagca tcgccgccgg
     2161 gcgcgcggcg tacgaggcgc acgacttcga cacggccaag aaggcgttcg acaccgcggc
     2221 gcggatcaaa ccgctcccgc ccgatgtgaa accgatctac gacgcggctt cggagcagtc
     2281 cgccagactg gaaggagcgc gggctctctt cgccgatcag aagtatcagg agacgatcgc
     2341 caccctcaat ccgctcctgc aggcagatcc gcagaaccag tcgatccgcc gcatgctcgt
     2401 gggcgcacac ttcaacctcg gcgccgcggc gctgcaggaa gagcgcgttc cggacgcgat
     2461 ccgcgagttc gacgaagtgc tgaagaacga cccgaatgac acgatggcga agcggagtaa
     2521 ggagcttgcc gagcgctatt ccggccagcc gaaagacctt ttgtataaga tttacgtaaa
     2581 atacctgccg ttgcgaaacg tgacgtaggc ggaatccggc gcgcgagcat gagctcgcgc
     2641 ccgcgatcgg gagggtgtga tgcgattcga tgagatcgag ctgcagtccg tgaccttgga
     2701 cgccgattcg cccacaaccg caccgccgtc accatcttcg atctcttcgt tcttccggcg
     2761 ggtggcagcg ctgctcatcg atctctcact ctttgcggcg ctgggaatgg cgctgtcgcc
     2821 actcttgccg tcgtcgatga gcgtgatctc gatcgcgtcg ctggccggct tcctcatcat
     2881 cgtcgcgttc tactacttcg ccggaacacg agcgctgtgg ggcaggacga tcggcggcgc
     2941 gatcttcgac gttcgcgccg cgcgaacacg gcgcacggcg cagcgatagg ctgcagttct
     3001 cgcccttttc tcgaccctac tctccaatcg agcgccttct cccccacgct tttgtggggg
     3061 agaaggtggc aaagccggat gagggggcgt ttgactaacg agacaccctt gcacaaacgg
     3121 caagcgccac atggatgcct tgtcaatcgg gcgcgagagg ctgctttccg catcttgcaa
     3181 cgccccccct catccgcctt cggcaccttc tccccccgaa aagcacgggg ggagaaggcg
     3241 ctcgattgga aggagtggcg atcaggacgt gagaaacagg gagaagaccc tagtcggagg
     3301 gagttgcgat aagagtcgaa gaagtcgaag aaatggtatg actgcgacgc ctctgttctt
     3361 cctactgact ggtgttcgga gagatgtgaa ccgaggcgat gcggttctgg gggtcaaagg
     3421 cgacgcgaaa tgccagagga gagcggtcgc agacggcgat cacgtcgatg atccggtatg
     3481 gccgctggac tcgctggcgc acttcggtga tggactggag cttcccgtat gtggtcaaga
     3541 gcttgttttt cagatctgtg aggcccggtg gcgtcagctg catgcgcatg gtgtcgtcaa
     3601 aatgttttga agcctcgtcg ac
//
LOCUS       DATLVW010000418         5117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_267316, whole genome shotgun sequence.
ACCESSION   DATLVW010000418 DATLVW010000000
VERSION     DATLVW010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267316"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2544)
                     /locus_tag="VNN25_12580"
     CDS             complement(<1..2544)
                     /locus_tag="VNN25_12580"
                     /inference="COORDINATES: protein motif:HMM:NF026035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXH92409.1"
                     /translation="MRFRITLFLALLLTGTTLFAVDIHDTRLLSDPAISGDRIAFGYA
                     NDLWVANLDGSGVRRLTSHPGIESGPRFSPDGKWIAFTGRYEGNTDVYIVPSEGGVPK
                     RLTWHPKNDVALGFTPDGANVVFTSPREVYTTRYLQLFTVPVGGGMATRLPIPHAAKA
                     VFSSDGKKIVYVPLGDAFNEWKHYRGGQVSNLVIFDTTTYATEHVPQPAGRCNDTDPM
                     WVDGKVYFRSDRDGEFNIYSYDPATKKVTRLTDHKDFPIVNASAGAGKIIYEQAGYIH
                     VLDPKTSATNRLKIGVAADLVETLPRFAKGKKYIRNAAVSPSGVRVALEFRGEILTVP
                     REKGDDRNLTNTPGANDRSPAWSPDGKSIAWLSDRSGEYRLYIGDQAGKGTPREIKID
                     GAGFYDDPKWSPDSKKISLADNSRSIYVVDVATGAADKVSSDVLYGPVRVLNHSWSPD
                     SQWLAYTRNTDTYMNRIYLYSIGEKKSYPLTDPYTDAGQPVFDPNGKYLYFLASTDAG
                     PVEDWFAQSNADLRATQSIYLAVLPKGVVSPIAKESDEEAAKSADESAMKPETKASDK
                     PADEKASADKTAKSAAKTVKVVIDFDGLTQRIVALPTKPAGYDSLQVGKSGQIFYLKS
                     GGSNRFGAEETSLTRFDLEKRKEETLLEKVDGYQLSADGKRMLVDKDGLSLIDAGDKL
                     DLTKFKLPIDRLQVKIDPRAEWRQIFDESWRINRDYFYDPNMHGADWNAMHAKYAVFL
                     PDLAVRQDLNRLMTWMHSELSVGHHRLSGGDSLAEVESIPGGLLGADYKVENGRYRFA
                     KVYGGLNWNPELRSPLTEPGVDVKAGEYLLAVDGKDLKPPEDLYSRFEQT"
     gene            2603..3298
                     /locus_tag="VNN25_12585"
     CDS             2603..3298
                     /locus_tag="VNN25_12585"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HXH92410.1"
                     /translation="MTKLLGLIGGISPESTIDYYRLILDAWRKATNDGSAPRILIDSL
                     NMQEALAYLNASDLDGLTEFLLIEIGRLHRAGADFGLITANTPHLVFDAVAARSPIPL
                     ISIVEATRDRAAASGFTKLGLFGTRFTMQARFFFDVFERAGMTLVVPDSDDQNYIHDK
                     YINELTAGVFNDATRDRMLGIARELVAKHSIQALILGGTELPLLLRDADIGVPLLDTT
                     RIHVGRAVEELLS"
     gene            3385..3762
                     /locus_tag="VNN25_12590"
     CDS             3385..3762
                     /locus_tag="VNN25_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXH92411.1"
                     /translation="MADHGDAALALVRSICLALPDTVEKLAWGAPTFRVHGRQFAMFL
                     NNHHGDGKIALWCAAPPGVQQELVAADPKHFYVPPYVGVGGWVGMRLDTKLRKGAIAA
                     IIEQRYRMIAEKKKASKRKASRL"
     gene            complement(3845..4243)
                     /locus_tag="VNN25_12595"
     CDS             complement(3845..4243)
                     /locus_tag="VNN25_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH92412.1"
                     /translation="MTTSARQFRVLVADDDPAIRSLVARIFIRRGYDVASASDGADAI
                     QKLDHDEPYDLLVLDLMMPRVDGVGVINHLAKRDGPRPAILVMTAAVPDILRRLPREH
                     VAKVISKPFDLDQLTQYADEAIRETNSSAS"
     gene            complement(4356..4793)
                     /locus_tag="VNN25_12600"
     CDS             complement(4356..4793)
                     /locus_tag="VNN25_12600"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4662..4664),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXH92413.1"
                     /translation="MLNVGDEAPDFTVSDHHGNTVSLHDLRGRKVVLWFYPKADTPGU
                     TLEGYGFRDLIGEYEKKNTTILGASFDNAADNAHFAEKFDFPFPLLCDTDRSIGIAYG
                     AADSPKDEYAKRIAYVIDEKGRIAEAHPKVNPQSYPKEQLARL"
     gene            complement(4919..>5117)
                     /locus_tag="VNN25_12605"
     CDS             complement(4919..>5117)
                     /locus_tag="VNN25_12605"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92414.1"
                     /translation="YQSIIASRVLGESNGAGEPLDGWLTARPRVVERIDQMMSDFRAA
                     QTVDLAMLTVASRQLRALVES"
BASE COUNT          892 a         1495 c         1689 g         1041 t
ORIGIN      
        1 ggtctgctcg aagcggctgt aaagatcctc gggcggcttg agatcctttc cgtcgaccgc
       61 gagcaggtac tcccctgctt tgacatcgac accgggctcg gtcagcggag agcgaagctc
      121 gggattccaa ttgagaccgc cgtagacttt cgcgaagcgg tagcggccgt tctcgacttt
      181 gtagtcagcg ccgaggagac cgccggggat cgactcgacc tcggcaagcg aatcgccgcc
      241 gctgagacga tggtggccga ccgagagctc cgagtgcatc caggtcatca agcgattcag
      301 gtcctgacgc acggccagat cgggaaggaa gaccgcgtac ttggcgtgca tggcgttcca
      361 atccgcgccg tgcatgttcg ggtcgtagaa gtaatcgcgg ttgatgcgcc acgattcgtc
      421 gaagatctgc cgccactccg cgcgcggatc gatcttcact tgcagtctgt caattgggag
      481 cttgaacttc gtgagatcga gcttgtcgcc ggcgtcgatt agcgacaagc cgtccttatc
      541 gaccagcatc cgcttgccat ccgcggagag ctggtaccca tcgacctttt cgagaagcgt
      601 ctcttccttt cgcttctcca gatcgaaacg cgtcaggctg gtctcctctg ctccgaaccg
      661 attcgacccg cctgacttga gatagaaaat ctggccgctc ttgccaacct gcagcgagtc
      721 gtagccggcc ggcttcgtcg gcagcgcgac gatgcgttgc gtgaggccgt cgaaatcgat
      781 gacgaccttg accgtcttcg cggctgattt ggctgtcttg tctgcgctgg ccttctcgtc
      841 ggcgggcttg tcgctcgcct tcgtctccgg cttcatggcc gattcgtcgg cactcttcgc
      901 cgcctcttca tcactctctt tggcgatcgg cgagacgaca cctttcggaa gaacggcgag
      961 gtagatcgac tgcgtcgctc gcaggtcggc gttcgattgc gcgaaccaat cctcgaccgg
     1021 accggcatcc gttgaagcga ggaagtagag atacttgccg ttgggatcga agaccggctg
     1081 accggcatcg gtgtagggat cggtgagcgg atacgacttc ttctcgccga tcgaatagag
     1141 gtagatccgg ttcatatacg tgtcggtgtt gcgcgtgtag gcgagccact gcgagtcggg
     1201 cgaccaggag tgattcagca cgcgcaccgg tccgtaaagc acatcgctcg acactttgtc
     1261 ggcggcgccg gtggcgacat cgacgacgta gatggagcgg gagttgtcgg cgaggctgat
     1321 cttcttcgaa tcgggcgacc acttcggatc gtcgtagaag cctgcgccgt cgatcttgat
     1381 ctcgcgcggc gttcccttcc ccgcctgatc gccgatgtag agccggtact cgccgctgcg
     1441 gtcggagagc caggcgatcg atttgccgtc gggcgaccag gccggcgaac ggtcgttcgc
     1501 gccgggtgtg ttggtgaggt tgcggtcgtc gcccttttcg cgcgggacgg tgaggatctc
     1561 gccccgaaac tccagcgcca cccgcacgcc cgaaggcgag accgcagcat tgcgaatgta
     1621 ctttttccct ttggcgaagc gcggcaacgt ctcgacgaga tcggcggcga cgccgatctt
     1681 caaccgattg gtcgccgatg ttttcggatc gagcacgtga atgtagccgg cctgttcgta
     1741 gatgatcttg cccgcgcctg cggacgcatt gacgatcggg aagtccttgt ggtccgtcag
     1801 ccgcgtcacc ttcttcgtgg cggggtcgta gctgtagatg ttgaactcac cgtcgcggtc
     1861 ggagcggaag tagaccttgc cgtcgaccca catcggatcg gtgtcattac agcggccggc
     1921 cggctgcggc acgtgctcgg ttgcgtacgt cgtcgtgtcg aagatgacga gattcgagac
     1981 ctggccgccg cggtagtgct tccattcatt gaaagcgtcg ccgagcggga cgtaaacgat
     2041 cttctttccg tcggaagaaa agacggcctt ggctgcgtgc gggatcggaa gccgcgtggc
     2101 cattccacct ccgaccggca cggtgaagag ttgcaggtag cgcgtggtgt agacctcgcg
     2161 cggtgaggtg aagacgacgt tggcgccgtc gggcgtgaag ccgagcgcga catcattctt
     2221 cggatgccac gtcagccgct tcggcactcc cccctcggac ggcacgatgt agacgtccgt
     2281 gtttccttca tagcgtccgg tgaaagcgat ccacttcccg tcgggcgaaa agcgcggacc
     2341 cgactcgatg cccggatgcg acgtcagccg ccgcacgccc gaaccgtcaa gattcgcgac
     2401 ccacaaatcg ttcgcgtagc cgaaggcgat gcggtcaccg ctgatcgcag gatcggagag
     2461 gagacgcgtg tcgtggatgt cgacagcgaa gagcgtggtt cccgtcagca gaagagcgag
     2521 gaacagggtg atgcgaaatc gcatgaaggc tccttttttg tacggccaga cgattcgcgg
     2581 actgtagcgt attctggcga tcatgacgaa gctcctcggc ctcatcggcg ggatcagtcc
     2641 ggaatcaacg atcgactatt accgcctcat cctcgacgcc tggcgcaagg ccacgaacga
     2701 cggcagcgcg ccccgcatcc tcatcgacag cctgaatatg caggaggcgc tggcgtacct
     2761 caacgcctcc gatctcgacg gcctgacgga gtttctgctg atcgagatcg gccgactgca
     2821 tcgcgccggc gcggacttcg gactgatcac cgcgaataca ccgcatctcg ttttcgatgc
     2881 cgtcgccgcg cgctccccaa tcccgctgat cagcatcgtc gaggccacgc gcgaccgcgc
     2941 ggcggcgagc gggtttacga agctggggtt gttcgggacg cgattcacga tgcaggcgcg
     3001 cttcttcttc gatgtgttcg agcgcgcagg aatgacgctg gtcgtgccgg attccgacga
     3061 tcagaattac attcacgata agtacataaa tgagctgact gcgggagtct tcaacgatgc
     3121 gacgcgcgac cggatgctgg gcatcgcgcg cgagctcgtg gcgaagcact caattcaggc
     3181 gttgatcctc ggcgggacgg aacttccgct gctactgcgc gacgcggata tcggggttcc
     3241 gttgctcgac acgacgagga ttcatgttgg gcgggcggtg gaggagttgc tttcgtaatt
     3301 gaccttgctc atcgggcaca ccaccggggc ggggacgccc cggtggccgc gagcgagacg
     3361 ctcgcactcc tttagaatca gcgtatggca gaccatggcg atgcggcgct cgcgctcgtg
     3421 cgaagcatct gtcttgcgct tccggatact gtcgagaagc tggcctgggg cgcgccgacg
     3481 tttcgggttc atggcaggca gttcgcgatg tttctgaaca atcatcacgg cgacgggaag
     3541 atcgcgctct ggtgcgcggc gccgcccggc gtgcagcagg agctggtggc cgcggatccg
     3601 aagcacttct atgttccgcc gtacgtcggc gtgggcgggt gggttgggat gcggctcgat
     3661 acgaagctgc gcaaaggcgc gatcgcggcg atcatcgagc agcggtatcg gatgattgcg
     3721 gagaagaaga aggcgtcgaa gcgtaaggct tcgcgattgt gagtgggttc ggcttgaatt
     3781 gaagtcgccg ccgggccggc ggcggtacag ccgtcgagcc gacggcgcta cgcacaccgc
     3841 agcgctacga ggcgctgctg ttcgtttcgc ggatggcttc gtcggcgtat tgggtgagct
     3901 ggtcgaggtc gaagggtttg gagatgactt tcgcgacgtg ctcgcgcggg aggcggcgga
     3961 ggatgtcggg gacggcggcg gtcatgacca gaatggcggg tcgcgggccg tcgcgtttgg
     4021 cgaggtggtt gatcacgccg acgccatcga cgcgcggcat catcagatca agcacgagga
     4081 gatcgtaggg ctcgtcgtgg tcgagcttct ggatggcgtc cgcgccgtcc gaggcgctgg
     4141 caacgtcgta gccgcggcgg atgaagatgc gggcgacgag cgatcggatg gccggatcgt
     4201 cgtcggcgac gagaacgcgg aactggcgag cagaggtggt cactgcgcgc gagcatgcaa
     4261 ggagcaagcc ccgaggagtg cgtgcgtctc gcacgcgggc attcgggcgt ctcgcccgaa
     4321 tgcttcgtgc gaccattgat aacaccgaag cgagatcaca acctcgccaa ctgctccttc
     4381 gggtagcttt gcggattcac tttcggatgc gcttcagcga tgcggccctt ctcgtcgatc
     4441 acgtacgcga tgcgttttgc atattcgtcc tttggtgaat cggcggcgcc atacgcgatc
     4501 ccgatactgc ggtcggtgtc gcagagcagc gggaagggga agtcgaactt ctccgcgaag
     4561 tgggcgttgt cggcggcgtt gtcgaaactc gctccgagga tggtggtgtt cttcttttcg
     4621 tactcgccga tgaggtcccg gaacccgtaa ccctctaacg ttcagccagg tgtgtcggcc
     4681 ttcggataga accagagcac gaccttgcgg ccgcgcagat cgtgaagcga aacagtgttg
     4741 ccgtggtgat ccgagacggt gaagtcaggg gcttcgtcgc caacgttcag cattcgtcct
     4801 cctcgcaatg atttcagcgc aaagcgtaac ttgcctgcaa tcgacacgcc gtcattgtag
     4861 tggggtcgca gagtgcggcg tcagctcggt gtcctcggtg tctctgtggt tttttcttct
     4921 agctctccac caacgcccgt aactgtctgc tcgcgaccgt cagcatcgcc aggtcgacgg
     4981 tttgcgcggc gcggaagtcg ctcatcatct ggtcgatgcg ctcgacgaca cgggggcgtg
     5041 ctgtcagcca tccgtcgagg ggttctccgg cgccgttcga ttcgccgagg actctcgagg
     5101 caatgatgct ctggtag
//
LOCUS       DATLVW010000419         7039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_470727, whole genome shotgun sequence.
ACCESSION   DATLVW010000419 DATLVW010000000
VERSION     DATLVW010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470727"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VNN25_12610"
     CDS             <1..536
                     /locus_tag="VNN25_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92415.1"
                     /translation="IIGFAGYGAYQIVTYDPVKSALAQIQKENPGAIVLQPLSPEVAA
                     QHKAQRDIEDAAWPHVLAAGYRSALEDIGKNWADEARGFIKSRRDAQTFLNIAEIPRR
                     LEIEAQCADDERLAKELIQLQPAFEQSGGVPPSEIAMVITKRNIEARPEAVCGRALIR
                     KAQAAEFRWLRDQLSVN"
     gene            588..1178
                     /locus_tag="VNN25_12615"
     CDS             588..1178
                     /locus_tag="VNN25_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92416.1"
                     /translation="MRKPILPFLILLFAAAALAQQTDRQIVHNRNAADIAGNYTPQWL
                     GDIAKEAAYKPSGSPAEQFAHAKSYFEPAAQLQAWLQEGEYRDAVLAGGADEQAIKKH
                     STDAAKRLADAAVLLLTKGGDLVSVAGDIRYLPISITTFSPRAKIATYALVALDFGGV
                     GDEAFRTKLEKLGGIIPDPDPSLPGYQEMMAPVAGD"
     gene            complement(1326..1910)
                     /locus_tag="VNN25_12620"
     CDS             complement(1326..1910)
                     /locus_tag="VNN25_12620"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXH92417.1"
                     /translation="MRAEPFIIWFIGVPAAGKTTIAAAVRDAVDARVEVLDSDEVRMR
                     LTPTPDWSDVERQLFYRALNEIAQRLVKHGISVLIAASGGGVDLDAFRERSVSRTFFV
                     HVDLDLSQAMKRRGGRLYRDASKGEIRLPLIRFRDEGPDGEDVAFCEEHGISAYELVL
                     PRKVDLTLDGALPPKVNAAAVLALLDADQTAMAP"
     gene            complement(1921..2505)
                     /locus_tag="VNN25_12625"
     CDS             complement(1921..2505)
                     /locus_tag="VNN25_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92418.1"
                     /translation="MIAVLESVPITEEQLEHLRYLHATAERYKDSIFNRAYTLMTAAG
                     IQVGLTAAFAALFRNTRGDLWATMLRADVAIVLLLNVCAAASALHAIMPHQSVSAVRL
                     LLSKLTREKQPARTLVADPLLSSFNRVCEMEKSAFMSQVGTASVSVLFADLAITYFNL
                     CHIVSARYASLRRAFIYEEIALLLWASVGVMAII"
     gene            complement(2596..4512)
                     /locus_tag="VNN25_12630"
     CDS             complement(2596..4512)
                     /locus_tag="VNN25_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92419.1"
                     /translation="MASVMPIDAAFITAFGIASCVGSLGVIDGHGHSTCSDGRRRIGE
                     LLGTDGAGQALILLTDHNTFTHFPRTLRLAMRYAPGRKHVLAPAIEVSCVAHDADLHV
                     ICVGGTGTAMEPVIRTLTEYYHERERLRLSAVQERFGVAIDYQLYERFVANRTPNWTL
                     SFRFLSRLVRSGKVPAEVLKAIRRGNEIGTDRLPIGSRPVSLAELLAVVAKSGGVAFL
                     CHVGDVAAHYGTVVVERVLTEHPTLHIDLSARPYHLSHADIVRSSSTLRRRLRNKPAL
                     RGSDFHDRPSPQTCPSRLRTLLARTAASRLGDLSAEFLIGHPIFLAPLVELPSLLQDV
                     PFVVPEGETIEQWIDRAFPSKGRRTAEVMLGVALLRDELGRAILIRASREGALRSSAS
                     AAAWLRVQPIEKVASQLRDAYATGWLAAMFDVAQRMGFLTILDRIAKPFLAYVPKDLG
                     DLYGTRATQTACARRIQNLAQEAAFDSAGLLIWRRKGLVSVYANLVRLLLSGKPARVG
                     ISCHDAARMAGDLLFRREVTDETRSRYYSELLFDHLAATLIIDPATTDIDYSALWDTI
                     SVPDVRQVEISPREAYHRRIWLHILKRDDDHVEPLELMIKTRRDFLVGRAVSMARQRV
                     FACSCGITLARRFI"
     gene            complement(4560..5162)
                     /locus_tag="VNN25_12635"
     CDS             complement(4560..5162)
                     /locus_tag="VNN25_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92420.1"
                     /translation="MRYNDVYVSLRVPIDDSVASQQSAICEAFNLIPRKELHVTIGFL
                     GTCEAEQIASFGAQLSAIAPSTLSTLRVLGVGGALQDESGKSLFIASGEVWQTRPRVL
                     WWAVECPEALIGFRNGMIAAALGVGLNASYLRPQYTPHITLGSAGPPTGHDWSLFDVH
                     DLPKDPTFRDLANPQTVSIDRLHVTSVERAPDSLFSVREY"
     gene            complement(6108..6383)
                     /locus_tag="VNN25_12640"
     CDS             complement(6108..6383)
                     /locus_tag="VNN25_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92421.1"
                     /translation="MTDIDVDHVLQLEQELEAAKDTTIKQLLERQKQTADQLKMLGYE
                     PKVAKPARSRKPCSKCQATDHDARFHRGDVSREQGGRKPVAAGPTAR"
     gene            complement(6397..>7039)
                     /locus_tag="VNN25_12645"
     CDS             complement(6397..>7039)
                     /locus_tag="VNN25_12645"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HXH92422.1"
                     /translation="SRAGLVERSRHAIDDPTDPFLRYQYCYRLSRMGQAAIGKGHAAI
                     EKSPASLAHELGITAFHIALSTGAPETHRIYWRQTDLKRTVNPDALFAITDTTRPPEN
                     STFYYFLEVERSRQGHYRRGESGLLTKLRRYGDYRRSEHCRKEWRHFADFRVIVVLQT
                     RERQTNLLRALKVNLPSPMIWTTTAEDIQRDICGAVFRAPPDFETAAHSLITK"
BASE COUNT         1552 a         2118 c         1969 g         1400 t
ORIGIN      
        1 ccatcattgg gttcgcaggt tacggcgcct accagatcgt gacgtacgac ccggtaaagt
       61 cggctcttgc gcagatccag aaggagaacc caggcgcgat tgtgcttcag ccgctttctc
      121 cggaggtggc agctcagcac aaggctcaga gggatattga ggacgcagca tggccgcacg
      181 ttctcgccgc gggctaccgc tccgctctcg aggatatcgg caagaactgg gccgatgagg
      241 ccaggggttt catcaaatct cgacgcgatg cccagacgtt cctgaacatc gctgagatac
      301 cccgacgtct cgaaatagag gcgcaatgcg ccgacgacga acgactcgcg aaggagctga
      361 tacagctcca acccgccttc gagcaaagtg gcggtgtgcc cccgtccgaa atcgcgatgg
      421 tcatcacaaa acggaacatc gaagccagac cggaagcggt ctgcgggagg gcattgatcc
      481 gcaaggcgca ggccgccgag tttcgatggt tgcgtgacca gctctccgtg aactagctgc
      541 tagcccaaca caacgtacaa ccgcagcact aacgaaagga gtcctacgtg cgaaaaccaa
      601 ttctcccgtt cctcattctc ctcttcgccg ctgccgccct agcgcagcag accgaccggc
      661 agatcgtaca caatcgcaat gccgccgaca tcgccggcaa ctacacgccg cagtggcttg
      721 gtgacatcgc gaaggaagcg gcctataagc cgagcggctc cccggcagag cagtttgctc
      781 atgccaagag ctacttcgag cctgcggcgc agctccaagc ctggctccag gaaggcgagt
      841 accgcgacgc ggtccttgcc ggtggcgccg acgagcaggc gatcaaaaaa cactccaccg
      901 acgcagccaa gcgccttgct gacgcggccg tcttgctcct cacgaagggc ggcgacctcg
      961 tatcggtagc cggagacatc cggtatctgc cgatcagcat tacaacgttt tcgccgagag
     1021 cgaagatcgc gacctacgcc ctcgttgcac tcgacttcgg cggcgtcggc gacgaagcct
     1081 tccgtacaaa gctcgaaaag ctcggcggga tcatccctga ccctgatccg agcttgcccg
     1141 gctaccagga aatgatggct ccggtagcgg gtgactgatc cacatcagta tctggcctcc
     1201 attggcccgg cgtagaccca caagtctgcg ccgttttctt ttgtcgggac ccacctctcg
     1261 taatcgtcac tttaacgccc cagttcagac gattcgctag ccggcggatg atgagccctc
     1321 gcgtctcaag gagccatcgc cgtttgatct gcgtccagca acgcgagaac cgcggcagcg
     1381 ttaaccttgg gtggtagggc gccatctagc gttaggtcga ccttccgtgg aagaacaagc
     1441 tcgtacgcag agattccatg ttcctcacag aacgcaacgt cctccccgtc aggtccctcg
     1501 tcacgaaaac gaatcagcgg cagccttatc tctcctttcg aagcgtcacg atagagcctt
     1561 ccgccacgac gcttcatcgc ttgcgacaga tccagatcaa cgtgcacaaa gaacgtgcgt
     1621 gaaaccgatc gttctcgaaa ggcgtccaaa tcgacgcctc ctccgcttgc agctattagc
     1681 accgaaattc catgtttcac caggcgttgg gctatctcat tcagcgcccg ataaaagagc
     1741 tgccgctcaa cgtcactcca atctggagtt ggcgtcaatc gcattcgtac ttcgtcactg
     1801 tcgagtacct ccacacgggc atccacggcg tcacgcacgg ccgccgcgat cgtagtcttc
     1861 cccgcagcag gaactccgat gaaccagatt atgaacggct ccgcgcgcat cgcccaacct
     1921 ctatatgata gccatcacgc cgaccgacgc ccacagcagc aacgcgatct cttcgtatat
     1981 gaacgcgcgc cgcaaggacg cataccgagc actgacaata tgacacagat tgaaatatgt
     2041 aatcgccaaa tccgcgaata ggacactaac cgacgcagta ccaacctgcg acataaaggc
     2101 gctcttttcc atctcgcaaa cacgattgaa ggaactcagt agtgggtcgg cgactaacgt
     2161 cctcgcgggt tgtttctcgc gtgtcaactt actaagcagc aacctcactg ctgacacact
     2221 ctgatgcggc attatggcgt gcagcgcact tgcagcagcg caaacgttaa ggagtagaac
     2281 gatcgcgacg tcggccctca gcatcgttgc ccacaaatcg cctcgcgtat tacggaacag
     2341 cgccgcgaac gcggcggtca gccctacttg gattcccgcc gccgtcatca gagtataagc
     2401 tctgttgaaa attgagtctt tatatctctc tgcagtggcg tgaaggtaac gaaggtgttc
     2461 tagttgttct tcggtgatgg gtacggattc taaaacggcg atcattgtat cgtctccaca
     2521 aacgcgcgac actcggacac catggcttcc tgaatcgatc cgctcacatc tagtcgctgg
     2581 cacgctaggc tgtaatcaaa taaaccgtct cgccagggtg atgccgcaac tgcaagcgaa
     2641 caccctttgg cgcgccattg atacggcgcg gcctaccagg aagtctcgcc gcgttttgat
     2701 catcaactca agcggttcaa cgtgatcgtc gtcacgcttt agaatatgca gccatatgcg
     2761 gcgatgatac gcctcgcgcg gggatatctc gacttgccgc acgtccggga ctgaaatcgt
     2821 gtcccacagc gccgaatagt caatgtcggt tgtcgcggga tcaattatta aggtcgcggc
     2881 gagatggtca aatagaagtt cggagtaata acgactgcgt gtctcatctg tcacctctcg
     2941 ccgaaaaagg aggtcgccag ccatgcgcgc cgcatcgtga cagctgattc ccactcgggc
     3001 cggcttaccg ctcaaaagca gtcgtacgag gttcgcgtaa acagatacaa gacctttgcg
     3061 ccgccaaatc agcaaaccgg cagagtcgaa cgccgcttcc tgagctaggt tctggatgcg
     3121 tcgagcgcac gccgtttgcg tcgctctcgt tccatacaaa tcgcccagat ctttaggaac
     3181 atacgctagg aatggtttag cgatcctgtc gagaatggtc aagaatccca tccgctgcgc
     3241 gacatcgaac atagcagcca gccaacctgt tgcataggcg tcccggagct gcgacgccac
     3301 tttctcgatg ggctgtacgc gaagccacgc tgccgctgag gcgctactac gaagagcgcc
     3361 ctcacgcgaa gcacggataa ggatggcccg cccaagttcg tcacgaagca aagcgacacc
     3421 caacattacc tcggccgtgc gccggccttt cgaaggaaaa gcgcggtcaa tccactgttc
     3481 aatggtctca ccctcgggaa ccacgaaagg gacatcctgg agaagcgaag gcagttccac
     3541 aagcggcgca agaaagattg ggtgtccgat caggaactcc gccgaaagat cgcctaagcg
     3601 cgaagcagcc gtccgagcta gcagagttcg gagccgagac ggacacgttt gggggctcgg
     3661 ccggtcgtga aaatcgcttc cacgtagtgc aggcttgtta cgcaaccggc ggcgaagtgt
     3721 ggatgatgaa cgcacaatgt ccgcatgact cagatgataa gggcgagcgg acaggtcgat
     3781 atgcaaggtc gggtgttcgg ttagcacccg ctcaaccacc accgtaccgt agtgcgccgc
     3841 cacatcgccg acatgacaaa gaaatgctac gccgccggat ttggcaacga cggccagtag
     3901 ttcggcaagg ctaaccggtc tggacccgat cggtagtctg tcagtgccga tctcattgcc
     3961 acgcctgatc gccttcagca cttcagccgg caccttgcca cttcttacca atcggctgag
     4021 gaagcgaaag gagagcgtcc agttcggtgt ccgattggcc acgaaacgct catacaattg
     4081 gtagtcgatg gccacgccga agcgctcttg cactgcggag agacgcaatc gctcgcgctc
     4141 gtggtagtac tcggtgagtg tgcgaatcac cggttccata gcggtgcccg tgccaccgac
     4201 acaaatcacg tgcaggtccg catcgtgcgc cacacatgac acctcaatcg caggcgccag
     4261 cacatgcttc cgcccagggg cgtagcgcat tgccaggcgc agcgttctcg ggaagtgcgt
     4321 gaatgtgttg tggtcagtga gtagaattaa agcctgaccg gctccgtctg ttccgagaag
     4381 ctcgccaatc cgccggcggc cgtcggaaca tgtcgaatgt ccgtggccgt cgattacgcc
     4441 gagcgaaccc acgcatgacg ctatgccgaa agcggtgatg aaggccgcgt cgatcggcat
     4501 gacgctggcc aaggcatcgg cgactgtccc agatgacttc atcgatgggt tgccgcgatt
     4561 cagtattcgc gaacggagaa aaggctgtcc ggtgcgcgct caacagaagt cacgtgcaac
     4621 cgatcaatgg aaaccgtttg gggatttgct agatcgcgaa acgtcgggtc tttcgggagg
     4681 tcgtgaacgt caaaaagaga ccagtcgtgc cccgtaggag gacctgcaga ccccagcgta
     4741 atatgtggcg tatattgtgg acgcagatag ctggcgttca gaccgacgcc cagtgcggct
     4801 gcgatcatgc cgttacgaaa tccgatgagc gcctctgggc actccaccgc ccaccacaag
     4861 acacgcggcc ttgtttgcca cacctcgcca ctggcgatga agagcgattt tcctgactcg
     4921 tcctgcaagg ctccaccaac gcccaagaca cgcagcgtgc tcaacgtgct tggcgcaatc
     4981 gcactcagct gagcgccgaa tgaggcgatt tgttcggcct cgcatgtgcc gagaaaccct
     5041 atcgttacgt ggagttcttt tcgggggatg agattgaagg cttcacaaat agccgactgt
     5101 tgtgaagcta cgctatcatc gataggtaca cgtagagaaa catagacatc gttgtatcgc
     5161 atagttctag cccttatcgc attacttcgc caacctgcgt gtgtaaccat gaggacacct
     5221 cgactaggcg acccgcatga tcaagcgtgg tgacatgtag taccttcgcg ccgagggaca
     5281 ccgcctgttc aaggaaggcc cgtcgaagtt cgggcgctac cgcgcgcgta agtttcggcg
     5341 tatacacgtc gagcggtttg cttgggtcaa ccgccgcccg tcgcgcgagc ttgtcgatga
     5401 tccaatcgct cggcgcatcc agaaaaacta tgagcgccgg cagctgcatg agagagatgg
     5461 cgctttcgta atgtgagcga agatccgcta cctcgatggc accaagggca ccgagagatt
     5521 cgtacgccct gagatacgca atccagtcgt agatacaccg atcctggagt ccgactggtc
     5581 cttgccgatt tctgagcaga aagcgttcgc gccgcgattc ccggagatga cgggcgaggt
     5641 tattccgcat ttgccgggac aacgacgggg attgagcgag gtcaagacgg tcgaggactg
     5701 caccgtttac acaactagct acgccacgga tcagcgtagt cttgccgaca ccgtgacagc
     5761 cagagactct cactacgaca gaacacttct gcgcgagcat ccctttaggc tcggagagcg
     5821 agggcgcgat gccaaactcc ctactactat tatgagtata gcaggccccg caaacttcca
     5881 agcgctatcc acccctctca gataggagtg acggacaggg gaatttccct acccatcgca
     5941 cttgatttgc ttttgcttgc ccgatcatcc caacccgctg cggtgagccg gagctcatag
     6001 ctccccgcgc aacggggagg tgcggttcgc ggcctgcggg tagtggagct actcgaagtt
     6061 atcgtgccgc agcggactca cgacgcagcc cgctgcggca cgattggtca acgtgcggtg
     6121 ggaccagccg cgacgggttt cctgccgcct tgttcacgcg aaacatctcc gcgatggaag
     6181 cgtgcatcat ggtccgtcgc ttggcacttt gaacaaggct tccggctgcg agccggtttt
     6241 gccacctttg gctcgtaccc gagcatcttc aattgatcgg cggtctgctt ctgtcgttcg
     6301 agcagctgct tgatcgtcgt gtctttggcg gcttcgagtt cctgttccag ttgtaacaca
     6361 tggtctacgt cgatgtcagt catgtctctc tcccctctac ttggtaatga gcgaatgcgc
     6421 ggctgtctca aagtcaggtg gcgcgcgaaa caccgcgccg cagatatcgc gctgaatgtc
     6481 ttcggcggtc gtggtccaga tcattgggct aggcaaatta accttgagcg cacgaaggag
     6541 atttgtctgc cgttcccgcg tctggagcac gacgatcact ctgaaatccg cgaagtgtcg
     6601 ccactccttg cgacagtgct ctgatcgccg gtagtcgcca tatcgccgaa gcttcgtgag
     6661 aagtcccgac tcgccgcgcc gatagtgtcc ttgccgcgag cgctcgacct cgagaaagta
     6721 gtagaacgtc gagttctccg gcggccgcgt cgtatcggtt atcgcaaaga gagcatcggg
     6781 attcaccgtc cgcttcagat cagtttgtcg ccagtagatg cggtgcgtct ccggcgcgcc
     6841 ggtgctcagc gcgatgtgga acgcggtgat tccgagctcg tgcgcaagcg acgccggaga
     6901 tttctctatg gcggcgtgcc ctttgccaat ggccgcctgt cccatgcgcg aaagccggta
     6961 gcaatattga tagcggagaa acggatctgt tggatcgtcg atagcgtggc gagagcgctc
     7021 aacaagtcca gcccttgac
//
LOCUS       DATLVW010000420         4077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_596259, whole genome shotgun sequence.
ACCESSION   DATLVW010000420 DATLVW010000000
VERSION     DATLVW010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596259"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(762..3677)
                     /locus_tag="VNN25_12650"
     CDS             complement(762..3677)
                     /locus_tag="VNN25_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXH92423.1"
                     /translation="MRSVCRVALIAALFLGNRAFALDPGRALTQARLSVWANGSGLPQ
                     ATIDAIVQTSDGYLWFGTEEGLVRFDGMRFVVHDRQNTPGLRSPFISSLYEAPDKTLW
                     IGTYGGGVARLRNGRIEAFHPELLGSDRIRTFHTMRDGAIFVATAGGGLLRFDGEKVT
                     RFTTRDGLPSDRIWTIADDGEDGLWVATYGGGAVRWRDGRVQQRITTREGLPNDVARA
                     LLRDPDGTLWIGTDGGGLAAWRAGSIVRTVTMRDGLPNAFVRTIHRSRDGSLWIGTDG
                     GLARLRGSRAESMGVAEGLPSAAIRSMIEDREGSLWIGTAGGLVRLSDTRVLSYTRKE
                     GLKVDTVRAIFEARNGGVWVGTEGGLCEVLPRRVQCRTEADGLPHGTVYALIESRDGS
                     LWVGMDGGGIARFRDGKFVETIDTRSAGLPSDRVRALVETAQGDLWVSMAAGLALVHN
                     GRAVRIKEFEDRQLRPLLALPDGNLLVGTDGAGLWRVSGDGSKVNVVASLGSGLQSDR
                     VFSLTMDAGKSGVWIGTSGGGLSRLDLADGSVRSLTRRDGLHDDVVFDVVDAGPGADL
                     WLTSNRGVYRVQRERVLAAMMGKKSDLTGTVYGTIDGMPSAECNGAFPGAMRSRDGRI
                     WIATARGLAVIDPKANIRNEVPPPVRVEEALIDGGVREAGSSLRVPPGAQRLELRYTA
                     LSLRAPELVTFRYMLEGFDRDWVEAGTNRVATYTKLAPGDYTFRVLATNEDGVRSSSE
                     ARLAVTVEPQWFETWWARLLALALLAAALWGIVRLRLVTLHARHAELEALVAERTSSL
                     AVANERLSGLSYRDGLTGVANRRRFDEALAEACAAARADGSPLSLVLLDLDHFKRLND
                     SQGHQEGDEALRSVATLLAGQTEVRGGLAARFGGEEFAWLLPGVTLNEARSEAETLRL
                     KIRDARIRHAESEQGVVTASFGVSGGTGMTPPALVAAADAALYRAKSGGRDRVESEG"
     gene            complement(3742..>4077)
                     /locus_tag="VNN25_12655"
     CDS             complement(3742..>4077)
                     /locus_tag="VNN25_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH92424.1"
                     /translation="IVSEPQRDWDMASLADVGHAAERHLLRLFVDHAGISPLHYLRLI
                     RLERARQSLEYGATVTRAAAVAGFGSDVQLRRAWSRQWGGSPRDAARAAESAASAPRS
                     PRTTRSRSR"
BASE COUNT          750 a         1391 c         1258 g          678 t
ORIGIN      
        1 gggaactatt tagttcgatc cgggaactat ttatctcgac ttgggatctg tgtagttcgg
       61 ctttcgaacg atgcagatca gcgtcggaac tatttcgatg atggctaaac tactggttgt
      121 acgctagtta tcgttttctc acgcgaagac gcgaagacgc gaaggacggg tagagcgctc
      181 gagccggtag gagccgctgg ggtcacacct tcgcactttg gccgttccgg atcattctcg
      241 cgcaagtttc tgtctgtgag tgacttacag cttggacaca attccactcg cttctggcct
      301 gcgaggatca accaagcacg caagcgatgc gtttgcaata acttaggcgc gaattcgggc
      361 tgagcgtttg caccacagag acacagaggt cacagagtag gattcaactc tgagtcctca
      421 gagaacgttg tcggagtgcg gtcgtctcga ccgcgggcac tcgggcgtcc tcgcccgggt
      481 gcggtcaggg cggaggtcga agagactggg gtcacgcctt cggccattgg tctccggaga
      541 atcaactaag cgggcaaacg acgtttgata caactcaagt ttcgaacttg gctgacgcaa
      601 atcaatgtgc gcgctcaaga agaccgacaa agaccgaagg tgtgacccca acgactgccg
      661 cttacgagca cgcattcaag aacgtcgacg aacttcgacc tccgcgctga cagccctcgg
      721 gcgaggacgc ccgagggccc gcggtcgaga cgaccgcact cctatccctc cgactccacc
      781 cgatcgcggc cgcccgactt tgcgcggtag agtgcggcgt cggcggcggc taccagggcc
      841 ggcggcgtca ttcctgtgcc gccggacacg ccgaagctcg cggtgacgac accttgctcc
      901 gactccgcgt ggcggatgcg agcgtcgcga atcttcaggc ggagcgtttc cgcttcggac
      961 ctcgcttcgt tcagcgtcac gccgggaagg agccaggcga actcctcgcc gccgaagcgc
     1021 gcggccagtc ctccgcgtac ttccgtctgc ccggcgagaa gcgtggcaac tgagcgcaag
     1081 gcttcgtctc cctcttgatg accctgcgaa tcgttgagtc tcttgaagtg atcgagatcg
     1141 agcaacacga gcgagagagg cgagccatcg gcacgagcgg cggcgcatgc ttccgccagc
     1201 gcctcgtcaa aacgacggcg gtttgcgacg cctgtcaatc cgtcccgata cgacaaaccg
     1261 ctgaggcgct cattggctac cgcgaggctg ctggtgcgct cggccacgag cgcttcgagc
     1321 tccgcgtgac gggcatgcag cgtcacgagg cgcagacgca caattcccca gagcgcagcg
     1381 gcgagaaggg ccagcgcgag gaggcgcgcc caccacgtct cgaaccattg cggctcaaca
     1441 gtgacggcca gccgcgcctc gctgctgctc cgcacgccgt cttcgttcgt agccaggacg
     1501 cggaacgtgt aatcgccagg agcgagtttc gtgtacgtcg caacacggtt cgttcccgcc
     1561 tccacccaat cgcggtcgaa tccctcgagc atgtagcgga acgtcaccag ctccggagcg
     1621 cgcagactga gagccgtgta gcgcagctcg agacgctgcg cgccgggtgg gacgcgcagt
     1681 gagctgccgg cctctcgaac gccgccatcg atcagcgcct cctccacgcg aacgggaggc
     1741 gggacctcat tgcgaatgtt cgctttgggg tcgatcacgg ccagacctcg cgcggtcgcg
     1801 atccagatcc gtccgtcacg cgagcgcatc gctccgggaa aggctccgtt gcactccgcg
     1861 gacggcatcc cgtcgatcgt cccgtaaacg gtcccggtca gatcggactt cttccccatc
     1921 atcgccgcga gcacacgctc gcgctgcacg cgatagacgc cgcgattgct ggtgagccag
     1981 agatccgcgc cagggccggc gtcgacgaca tcgaagacga cgtcgtcatg caggccatcg
     2041 cgtctggtga gcgatcgaac ggagccatcc gcgagatcga gacgcgacag accgcctcct
     2101 gatgttccga tccacacgcc gctcttgccg gcgtccatgg tcaggctgaa cacccggtcg
     2161 ctttggaggc cgctgcccag actcgccacc acattcacct tcgacccatc gccgctcacg
     2221 cgccacagtc cggcgccgtc cgttccgacg aggagattgc catcgggcag cgccagaagt
     2281 ggccggagct gccggtcctc gaactccttg atgcggaccg cccgcccgtt gtgaacgagc
     2341 gccagaccgg cggccatgct gacccagaga tccccttgcg ccgtttcgac gagagcgcgc
     2401 acacgatcgc ttggcagacc cgcgctgcgc gtatcgatcg tttctacgaa cttgccatcg
     2461 cgaaaccggg cgattccgcc tccgtccatt ccgacccaga gactcccatc gcggctctcg
     2521 atcagcgcgt acaccgtgcc gtgcggaagc ccgtctgcct ccgtgcgaca ctgcacgcgg
     2581 cggggcagaa cctcgcacaa tcccccttcc gtcccgaccc agacgccgcc gttgcgcgct
     2641 tcgaaaatgg cgcggacggt atcgaccttc aatccctcct tccgcgtgta ggagaggacg
     2701 cgcgtgtcgc tcagcctgac cagcccaccg gctgtgccaa tccagaggct tccttcgcga
     2761 tcctcgatca tggagcggat ggcggcgctt ggcaggcctt ccgccacgcc catcgattcg
     2821 gcccgcgatc ctcgcaggcg tgccagcccg ccatccgtac cgatccaaag gcttccatcg
     2881 cggctgcgat gaatcgtccg gacgaacgcg ttcggcagcc cgtcgcgcat cgtcaccgtg
     2941 cgcacgatcg accccgcgcg ccatgcggcc agaccgccgc cgtcggttcc gatccacagc
     3001 gtgccatcgg gatcgcgcag aagcgcgcgc gcaacatcgt tcggcagacc ttcgcgggtc
     3061 gtgatccgct gctgtacgcg tccgtcgcgc cagcgaaccg ctcccccacc gtacgtggca
     3121 acccacaagc cgtcttcgcc gtcgtcggcg atcgtccaga tccggtcgct cggcagcccg
     3181 tcccgagtcg tgaagcgcgt gaccttttcg ccgtcgaagc ggagcagccc accacccgcc
     3241 gtggcgacga agatcgctcc gtcgcgcatc gtatgaaacg ttcgaatgcg atctgacccc
     3301 agcagctccg gatgaaacgc ctcgatccga ccgtttcgaa gacgagccac tccgccgccg
     3361 taggtgccga tccacaacgt cttgtccggc gcttcgtaaa gcgaggagat gaacggactg
     3421 cggagtcccg gcgtattttg ccggtcatgg acgacgaacc gcattccatc gaaccggacc
     3481 agcccctcct cggtgccaaa ccacaagtac ccatccgacg tctgaacgat ggcgtcgatc
     3541 gtcgcctgcg gcagccctga accattcgcc cagacagaaa ggcgagcctg cgtcagcgcc
     3601 cgcccaggat cgagcgcgaa ggcacggttt ccgagaaaga gcgcggcgat cagcgcaacc
     3661 cggcaaacac tacgcaaact ggctgatgaa atccgcgcct ccgaaggtgt gaggcatcgt
     3721 acaccgaccg aagcgagcgt ctcagcgact gcgagatcga gtcgtgcgcg gtgatcgagg
     3781 tgccgaggca gctgattccg ccgcacgcgc cgcatcgcgg ggcgagccgc cccactggcg
     3841 gctccaggcg cggcgcagtt gcacgtcgga gccgaagccg gcgacggcgg cggcacgcgt
     3901 gacggtggcg ccgtattcga gggactgtct tgcgcgctcc aggcggatca ggcggaggta
     3961 gtgcagcggc gagatgccgg cgtggtcgac gaacagccgc aagaggtgtc gctcggccgc
     4021 gtggccaacg tcggccagcg aggccatgtc ccagtcgcgc tgcggctcgg agacgat
//
LOCUS       DATLVW010000421         8330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_307939, whole genome shotgun sequence.
ACCESSION   DATLVW010000421 DATLVW010000000
VERSION     DATLVW010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307939"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VNN25_12660"
     CDS             <1..371
                     /locus_tag="VNN25_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92425.1"
                     /translation="VFLRNTRLGPSPLYFDQGTYNVAADEHGQKIIVPMPSNVIHIDT
                     QRAMAVAPADEPRTLDAFKAAVNESMRGTQRRLEMNAMAKVPKTKLQSSIWSTLAANK
                     LLVALALVGIAVAAWQLWRR"
     gene            complement(479..931)
                     /locus_tag="VNN25_12665"
     CDS             complement(479..931)
                     /locus_tag="VNN25_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HXH92426.1"
                     /translation="MKKKSSPTPDPRKPTPAAGYSGTPLAAKLGMKPGFSVLVVNEPA
                     NYGLLLAPLPEPVTFVKKLTNSVDLIHLFTKSAAELDAKLRVWRHSIKTDATIWISWP
                     KKASKVATDITEDVIREVALPLGFVDVKVCAVDETWSGLKLVIRKELR"
     gene            complement(1020..1655)
                     /locus_tag="VNN25_12670"
     CDS             complement(1020..1655)
                     /locus_tag="VNN25_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92427.1"
                     /translation="MKRLIVLVLLLCAACGGEKPESETTTLPASPPQRHTGPGGIIPS
                     PLPVPVHKPNRSTPQQCAGDGSYEQALDCFRISSGFPFILENPHGVQAQGKMTRSTPG
                     MERVQFKASDGASWTGEAKAAGVVWSRNGKHEQSPPDITNRVWQRTTMVLDPMKKEGT
                     PPPTFEKSGGEMLNHYHFTNANSGEANDVWISTKDGRIVKWTAGDSTLRIK"
     gene            complement(1652..3253)
                     /locus_tag="VNN25_12675"
     CDS             complement(1652..3253)
                     /locus_tag="VNN25_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH92428.1"
                     /translation="MRKLFIVALFFVATTTFAAPPAYAPNRKAGEGEGPFTRLIIRGA
                     TLVDGTGAPPIGPVDIVIEGNRIREVRSVGFPKVPIKADRRPKDAMKEIDGTGLWVLP
                     GFVDMHGHAGGEEQGTTAEYVFKLWMAHGITSVREPGCGNGVDWCLHERERSAKNEIV
                     APRLFPYVFTSHQQWEGGAIDSPEQARKFAQFVAKKGADGLKILSTGDPVFDPEILAA
                     LIDEANKLHLGTTTHLAQMGVARANIIDASRMGLRGMEHWYGLPEALFTDRTIQDYPR
                     DYNYNDESNRFGEAGRLWKQAAEPGSEKWNAVIDELVAKKFNIDPTLTIYEASRDLMR
                     AMRAEWHDRYTLPSLWRWYQPSRTAHGAYWFNWTTADEVEWKNNYRIWMRFLNDYKNR
                     GGHVTTGSDSGFIFKLYGFDYIRELELLQEAGFHPLEVIRSATMWGAEAINAPRGTKV
                     EFGIVRPGMLADLVLVDQNPIDNLKVLYGTGWLHVNDTTGNAERIGGIKYVMKDGILY
                     DAKQLLRDVATIVDQAKAAEAKNPK"
     gene            complement(3348..4268)
                     /gene="coaA"
                     /locus_tag="VNN25_12680"
     CDS             complement(3348..4268)
                     /gene="coaA"
                     /locus_tag="VNN25_12680"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HXH92429.1"
                     /translation="MSDVSPYLTFDRAAWSGLRGETPMTLSPDDVAELSGINEALSMK
                     EVEEVYLPLSRLLNLHVAAFDQLHRVSSRFLGAESPKVPYVVAIGGSVSAGKSTTARV
                     LRTLLSRWPDHPRVDLVTTDGFLFPNATLEERGIMHRKGFPESYDRPRLIEFVSQLKS
                     GVSPLAVPLYSHFAYDIIGEEQVLDRPDVVILEGLNVLQSGSIHSVFVSDFVDFSIYV
                     DASADDLETWFLDRFQKLRETAFRDPASFFHAFAAMPDEEAIAMAKEIWQRINLANLH
                     ENILPTRERATLILGKGASHVVERVLLRRL"
     gene            complement(4402..5358)
                     /locus_tag="VNN25_12685"
     CDS             complement(4402..5358)
                     /locus_tag="VNN25_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXH92430.1"
                     /translation="MILTRDDVRRLLDLDDCIAAVAEVFRKSARGEISPAGVLSMHAE
                     GGGFHVKAAMLDDGEERWFAAKTNANFPGNAARFGLPTIQGVVLLFDAIAGRPLAILD
                     SIELTALRTAAATAVAARHLARASSSTVTICGCGAQSAAQLLALARVLPLSEVLAFDI
                     DSERAAAFAAGMADAPFKVKAASDLRAALRTSDVVVTCTTSRRAFLMRDDVMPGTFVA
                     AVGADNPEKQELDPFLMACNKVVVDSIDQCAAFGDLHHAISAGAMSADDVYASLGEIV
                     AGDLPGRTNDEEITIFDSTGTAIQDVAAAVLVYKRAEAEIRD"
     gene            complement(5414..6331)
                     /locus_tag="VNN25_12690"
     CDS             complement(5414..6331)
                     /locus_tag="VNN25_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXH92431.1"
                     /translation="MAPEERRWLLLIHQLPPEPAYLRVRVARRLQRIGAIAIKNTVYV
                     LPDNRATREDFEWTVNEIREGGGEANLFEARVVAGLADAEIEQLFNAARNEDYDAVAA
                     DAGSRELAADVARLKKRLAEIRAIDFFGAAQGAAASARIAAMARRIAGEESPMQVIDD
                     AAAYTNRTWVTRSGVHVDRMASAWLIRRFIDPEAEIRFVAARNYRPRAGEIRFDMMGG
                     EFTHVGNRCTFEVMAARLVPHDRAVRAIGEVIHDIDLKETAFDRPETAGIGLLIAGIA
                     MGHSDDEARIERAAAAFDDLYAYYQRKER"
     gene            6367..7572
                     /gene="chrA"
                     /locus_tag="VNN25_12695"
     CDS             6367..7572
                     /gene="chrA"
                     /locus_tag="VNN25_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXH92432.1"
                     /translation="MTPRFAPFLLYFLRLGALGFGGPAALANAMRRDLVEQRGWITSD
                     EYESGLALAAACPGPLAYQLGVYCGYIRFGVGGGVAVAIAFGLAPFVLVTIAAALYVR
                     FSGSWEVRALFYGIAPAVVALIANACLNLGARTLRRDRLAWLFAAVACAVTVILQREL
                     APLFMVAGVIGAFVFAPRTEDSGERRSDSPVRQNAPPVGLESPTYVCQPPSRDEKRML
                     LAPAALFASAPAKLFLFFFKTGLLVFGSGLVIVPFLKTQVVDEYHWLTMRQFLDSVAI
                     GMISPGPVVITATFVGYLMSGFTGALAATLGIFMPSVLFTVIATPILLRHRANRRLAG
                     FVRGVGVTVVGVLFGTTVLVARDALGDLLTIAIALIAFAIVAFWKRIPEPFVILAAGV
                     AGIIAFASR"
     gene            complement(7550..8038)
                     /locus_tag="VNN25_12700"
     CDS             complement(7550..8038)
                     /locus_tag="VNN25_12700"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXH92433.1"
                     /translation="MNTRRRIALAAIIVLTIALDQGTKLIARQTLMNAFPRHYAGVLT
                     LLYTENPGAFLSLGSNLPVNVRHLIFDGLVSVGLLAAAFALFTGRMGLERDAVTIAFI
                     IGGGAGNLIDRFRFGGLVTDFIYLAIGPLHTGVFNIADMAITGGVIAIAILWAVSAMR
                     KR"
     gene            complement(8064..>8330)
                     /locus_tag="VNN25_12705"
     CDS             complement(8064..>8330)
                     /locus_tag="VNN25_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92434.1"
                     /translation="RGKGVQTIKLHKTELFPEKVVGTAVVGEAGTLVVQAGKRHTNLK
                     GADLEPYIGKRAQRGLKLPRGFQSVSSIHAWSDRELRNGGDLVM"
BASE COUNT         1459 a         2790 c         2485 g         1596 t
ORIGIN      
        1 tcgtcttcct gcgcaacacg cgcctcgggc cgagcccgct ctacttcgac cagggaacct
       61 acaacgtcgc tgcggacgag cacggtcaga agatcatcgt gccgatgccg tcgaacgtca
      121 tccacatcga tacccagcgc gccatggccg tcgcgcccgc cgacgagccg cgcaccctcg
      181 atgcattcaa agccgcggtc aacgagtcga tgcgcggcac gcagcgacgc ctggaaatga
      241 atgcgatggc gaaggtgccg aagaccaagc tgcagtcatc gatctggtcg acgctcgccg
      301 ccaacaagct gctcgtcgcg ctggcgctcg tcggaatcgc agtcgccgcc tggcagctat
      361 ggcgccgctg acgagaccag acttctcacg cgaagacgcg aacacgcgaa ggtgttgcga
      421 tggtctcctt cgcagcttcg cgtgagaagt gggttgcggg cgaagtcagc gctgtgcatc
      481 agcgcagctc cttccgaatg acaagcttca accccgacca cgtttcatcc accgcgcaaa
      541 ccttcacatc gacgaatccc agcggaagcg caacctcgcg aatgacatcc tcggtaatgt
      601 cggtcgctac cttcgatgcc ttcttcggcc aggagatcca gatcgtcgcg tcggtcttga
      661 tcgagtgacg ccacacgcgc agcttcgcgt cgagctccgc ggctgacttc gtgaagaggt
      721 ggatgagatc gacagagttg gtcaacttct tgacgaacgt cacaggctca ggcaacggcg
      781 caaggagtaa cccgtaattc gccggctcgt tgacgacaag cactgagaat ccgggcttca
      841 tgccgagctt tgccgcgaga ggcgttcccg agtagccggc ggcgggggtc ggttttcggg
      901 ggtcgggagt cggagaggac ttcttcttca ccgtctcaga cctccgctac ctggcgaccg
      961 agatccgcaa ccaatcttca tttccttctc cgactcccga ctcccgaccc ccgacaccct
     1021 cacttgatcc gcaacgtcga atcacccgcc gtccacttca cgatccggcc atctttcgtg
     1081 ctgatccaga cgtcgttcgc ctcgccgcta ttggcgttcg tgaaatggta gtggttcaac
     1141 atctcgccgc ccgacttctc gaatgtgggc ggcggcgtgc cctccttctt catcggatcg
     1201 agcaccattg tcgtccgctg ccagacgcga ttggtgatgt cgggcggcga ctgctcgtgc
     1261 ttgccgttgc ggctccagac gacgccggct gccttcgcct cgcctgtcca cgaggcaccg
     1321 tccgaagctt tgaattgcac gcgctccatc cccggtgtgc tgcgcgtcat cttcccctgc
     1381 gcctgaacac cgtgcggatt ctccaaaatg aatggaaagc ccgacgagat ccggaaacag
     1441 tccagcgcct gttcgtagga gccatcgcct gcgcactgtt gcggtgtgga gcggttcggc
     1501 ttgtgaacag gcaccggcag cggcgaaggg atgatgccgc cggggccggt atgccgctga
     1561 ggcggcgacg caggcaacgt cgtcgtctcc gattcgggtt tctcaccgcc gcaggcggcg
     1621 cagagcagta acacgagaac gatcaatcgt ttcatttcgg gttcttcgcc tccgccgcct
     1681 tggcctgatc aacgatcgtc gcgacatcgc gcagtagttg cttcgcgtcg tagaggatgc
     1741 cgtctttcat cacgtacttg atgccgccga tacgttcggc gttccccgtg gtgtcattca
     1801 cgtgaagcca gcccgtgccg tacagcacct tgagattgtc gatcggattc tgatcgacga
     1861 gaacgagatc ggcgagcatg cccgggcgga cgattccgaa ctcgaccttc gtgcctcggg
     1921 gcgcgttgat cgcctccgct ccccacatcg tcgccgagcg gatgacctcg agtggatgaa
     1981 accccgcttc ctgcaacagc tccagctcgc ggatgtagtc gaatccgtag agcttgaaga
     2041 tgaagccgga atcggagcca gtcgttacat gcccgccccg gttcttgtag tcgttcagga
     2101 agcgcatcca gatgcggtag ttgttcttcc actccacctc gtccgcggtc gtccagttga
     2161 accagtacgc gccgtgcgcg gtgcggctcg gctgatacca gcgccagaga gaaggcaacg
     2221 tatagcgatc gtgccattcg gcgcgcatgg cccgcatgag atcgcggctg gcctcgtaga
     2281 tcgtcagcgt cggatcgatg ttgaattttt tcgcaaccag ctcgtcgatg acggcgttcc
     2341 acttctcgct gcccggttcc gcggcctgct tccagagccg gcccgcctcg ccgaatcgat
     2401 tcgactcgtc gttgtagttg tagtcgcgcg gatagtcctg aatcgttcga tccgtgaaaa
     2461 gcgcctcggg caaaccgtac cagtgctcca tgccgcgaag acccatgcgc gaggcatcga
     2521 tgatgttcgc gcgtgctacg cccatctgcg cgagatgggt ggtggtgccg aggtggagct
     2581 tgttggcttc gtcgatgagg gcggccagga tctcgggatc gaagaccgga tcgccggtgc
     2641 tgaggatctt caatccgtca gccccttttt ttgccacgaa ctgcgcgaac ttgcgcgcct
     2701 gctcaggcga atcgatcgcg cccccctccc attgctgatg gctggtgaag acgtagggaa
     2761 aaagacgcgg cgccacgatc tcgttcttcg cactgcgctc gcgctcgtgc agacaccagt
     2821 cgactccgtt gccgcagccc ggctcccgga cgctggtgat gccgtgcgcc atccagagct
     2881 tgaagacgta ctccgcggtc gttccctgct cttcgccgcc ggcgtgcccg tgcatgtcga
     2941 cgaaaccggg cagcacccag aggccggtgc cgtcgatctc tttcatcgca tctttcggcc
     3001 tgcgatcggc tttgatcggg actttcggga agccgacgct gcgcacttcg cggatgcgat
     3061 tcccttcgat gacgatgtcg accggcccga tcggcggcgc gccggtacca tcgacgagcg
     3121 tcgccccccg gatgatcaac cgcgtgaacg gcccctcccc ttcgcccgct ttgcgattgg
     3181 gcgcgtaagc gggaggtgcg gcgaaggtgg tggtggctac gaagaacaga gcgacgatga
     3241 acagcttccg catgctctga ttctatccgg tcacgcgaag ccgctcacgc gaagacgcga
     3301 agacgcgaag ttggatccta acttcgcgtc ttcgcggctt cgcgtgatca caacctgcgc
     3361 agcagcactc gctccaccac atggctcgcg cccttgccca gaatcagcgt ggcccgctcg
     3421 cgcgtgggca gaatgttctc gtgcaggttg gcgagattga tcctctgcca gatctctttc
     3481 gccatcgcga tcgcttcttc gtccggcatc gccgcgaacg cgtgaaagaa ggatgcagga
     3541 tcgcggaagg cggtctcgcg gagtttctgg aagcggtcca gaaaccacgt ctccagatcg
     3601 tccgccgagg cgtcgacgta gatcgagaag tcgacaaagt ccgacacgaa gacggaatgg
     3661 atcgagccgc tctgcaacac gttcaaccct tccaggatca caacatccgg acgatcgagc
     3721 acctgctctt cgccgatgat gtcgtacgcg aagtgcgagt acagcggcac ggccagcggc
     3781 gagacgcccg acttcagttg cgacacgaac tcgatgagcc gcggtcggtc gtagctctcg
     3841 ggaaagccct tgcggtgcat gatcccgcgc tcttccagcg ttgcgttcgg aaaaagaaac
     3901 ccgtccgtcg tcacaaggtc gacgcgcgga tgatcgggcc agcgggacag cagcgttcgc
     3961 aacacacgcg cggtcgtgct cttgccggca gagacgctcc cgccgatggc aacgacgtac
     4021 ggaaccttcg gagactcagc gccgaggaag cgggaggaga cgcggtggag ctgatcgaaa
     4081 gcggctacgt gaagattgag caggcgcgaa agaggaaggt agacctcttc cacttccttc
     4141 atcgacagcg cctcgttgat cccgctcaac tccgcgacat cgtcgggcga gagcgtcatc
     4201 ggggtttccc cgcgcaaccc cgaccaggcc gcgcgatcga acgtgagata gggcgaaacg
     4261 tcgctcatgc gcggcggagt gtagtggaga agcagagatc agagatatca gagatcagga
     4321 agctggttta gtctctgatc tctggtccct agttagtccc tagtctctgg tctctagtct
     4381 ctggtctcta gtctctgatc tctaatctct gatctctgct tctgcgcgct tgtacacaag
     4441 caccgcagca gccacatcct gaatcgccgt ccccgtgctg tcgaaaatcg tgatctcttc
     4501 gtcgttcgtg cggcccggaa gatcgccggc cacgatctcg ccgagtgacg cgtagacatc
     4561 gtctgccgac atcgcgccgg cgctgatcgc gtgatgcaga tcgccaaacg cggcacactg
     4621 gtcgatcgaa tcgacgacga ccttgttgca cgccatcagg aaaggatcga gctcctgctt
     4681 ctccggattg tccgcaccga ccgcggcgac gaacgtgccc ggcatgacgt cgtcgcgcat
     4741 cagaaacgcg cggcgcgagg tcgtgcaggt caccacgacg tcgctggtac ggagcgcagc
     4801 ccgaagatcg ctcgccgctt tcactttgaa cggcgcatcg gccatcccgg ccgcaaaggc
     4861 agcagcacgc tcgctgtcga tgtcgaacgc gagcacctcg gacaacggca acacgcgcgc
     4921 gagtgcaaga agctgcgcag ccgactgcgc gccgcagccg cagatcgtga cggtcgacga
     4981 cgacgcccgc gccagatgcc gcgccgccac cgccgtggcc gccgcggtgc gaagcgccgt
     5041 cagctcgatc gaatcgagga tcgccagcgg gcgcccggca atcgcatcga acagcagcac
     5101 gacgccctga attgtcggca gtccgaatcg cgccgcgttg cccggaaagt tcgcattcgt
     5161 cttcgccgcg aaccatcgct cctcgccatc gtcgagcatc gccgccttga cgtgaaaccc
     5221 gcccccctcg gcgtgcatgc tcaacacacc tgcgggcgaa atctcgccgc gcgccgattt
     5281 gcgaaatacc tccgcgacgg cggcgatgca atcatcgaga tcgagcaggc ggcgcacgtc
     5341 gtcgcgcgtg agaatcatgg gaccggaatt gctcatcttc acgagttgct gagaagcagg
     5401 atgcgcaagc acgtcatcgt tcctttcgct ggtagtacgc atagagatcg tcgaacgccg
     5461 ccgcggcccg ctcgatgcgc gcttcgtcgt cagagtgacc catggcgatt ccggcaatga
     5521 gcagaccgat cccggctgtt tccggacggt cgaacgccgt ctccttgagg tcgatgtcgt
     5581 gaatcacctc gccgatcgcg cggacggcgc ggtcgtgcgg cacgaggcgt gcggccatca
     5641 cctcgaacgt gcagcggttc ccgacatgcg tgaactcgcc gcccatcatg tcgaagcgga
     5701 tctcgcccgc acgcggcctg taatttcgtg ccgccacgaa ccggatctcg gcctcgggat
     5761 cgatgaaacg gcggatgagc cacgccgagg ccatgcggtc gacgtgaacg ccactccggg
     5821 ttacccaggt gcggttcgtg tacgccgcgg catcatcaat cacctgcatc ggactctcct
     5881 cgccggcgat cctccgtgcc atcgccgcga tccgagcact tgccgcggcg ccctgggccg
     5941 cgccgaagaa atcgatggcg cggatttcgg ccaaccgctt cttcagccgg gccacgtcgg
     6001 cggccagctc gcgcgaccca gcatcggccg cgacggcgtc gtagtcttcg ttgcgcgccg
     6061 cattgaaaag ctgctcgatc tccgcatcgg cgagaccggc cacgacgcgc gcctcgaaga
     6121 ggttcgcctc gccgccgcct tcgcggatct cgttcacggt ccattcgaaa tcctcacgcg
     6181 tcgcgcgatt gtcggggagg acgtagaccg tgttcttgat ggcgatcgcg ccgatgcgtt
     6241 gcagtcgcct tgctacgcgg acgcgcaggt acgccggctc tggcgggagc tggtggatca
     6301 gcagcagcca gcgtctctcc tcgggtgcca tgacacaact gtatcactag atgctacagt
     6361 tacagcgtga cgcctcggtt cgctccgttc ctcctctact ttctccgcct cggcgctctc
     6421 ggcttcggcg gccccgcggc tcttgccaac gccatgcgtc gcgacctcgt cgagcaacgc
     6481 ggctggatca caagtgatga atacgagtcc ggcctcgccc tcgccgccgc ctgtcccggc
     6541 ccgctggcct atcaactcgg cgtctactgc ggctacatcc gtttcggcgt cggcggcgga
     6601 gttgctgtcg ccatcgcgtt cggactggcg cccttcgttc tcgtcacgat cgccgccgcg
     6661 ctctacgttc gcttcagcgg cagttgggaa gtgcgcgcgc ttttctacgg catcgccccg
     6721 gccgtcgtcg cgctcatcgc caacgcctgc ctcaacctcg gcgcccggac acttcgccgc
     6781 gaccgactgg cctggctctt cgcggcggtg gcctgtgccg tgactgtgat tctgcaacgc
     6841 gagctcgcgc cgctgttcat ggtcgcggga gtcatcggcg cattcgtctt cgcaccgcga
     6901 acggaggatt ctggcgaacg taggtcggac tctccagtcc gacaaaacgc gccgccagtc
     6961 ggactagaga gtccgaccta cgtttgccaa ccgccgtctc gcgatgagaa gaggatgctt
     7021 ctcgcacctg cggccctatt cgcgagcgca cccgcgaagc tcttcctctt cttcttcaag
     7081 accggacttc tcgtcttcgg cagcggcctc gtgatcgtcc cctttctgaa gacgcaagtc
     7141 gtcgatgaat accactggct gacgatgcgc cagtttctcg actccgtcgc catcggcatg
     7201 atctcgcccg gcccggtcgt gatcaccgcg acgttcgtcg gctacctgat gagtggattc
     7261 accggcgcgc tggcagcgac cctcggcatc ttcatgcctt cggtgctctt caccgtcatc
     7321 gccacgccga tcctgctgcg gcatcgcgcg aaccggcggc tggccggctt cgttcgcggc
     7381 gtcggtgtga ccgtcgtcgg cgtgctgttc ggcaccaccg tgctcgtcgc ccgcgacgcc
     7441 ctcggcgacc tgctcacgat cgcgatcgcc ctgatcgcct tcgccatcgt cgcgttctgg
     7501 aaacgcatcc ccgagccctt cgtgatcctc gctgccggag tcgccggcat catcgctttc
     7561 gcatcgcgct gaccgcccac aagattgcga tcgcgatcac gccacccgtg atggccatgt
     7621 cggcgatgtt gaacacgccc gtatgcagcg gccctatcgc gagatagatg aagtccgtga
     7681 cgagcccacc gaatcggaag cgatcgatca gattaccggc gcccccgccg atgatgaacg
     7741 cgatggtcac cgcatcgcgc tccaatccca ttcgtccggt gaagagcgca aacgccgccg
     7801 cgagcagtcc gaccgagacc agcccgtcga agatgagatg cctcacgttg acgggcaggt
     7861 tcgagcccaa cgagagaaac gcgcccggat tctcggtata gagcagcgtg agcacccccg
     7921 cgtagtggcg cgggaacgcg ttcatcagcg tttgccgggc gatcaacttc gtcccctgat
     7981 caagcgcgat cgtgagaacg atgatggcag cgagtgcgat acgccgacga gtgttcattc
     8041 tatttttcgc gcgaagcgca aagctacatc acgagatcgc ccccgttgcg caactcccga
     8101 tcgctccacg cgtggatcga tgacacgctc tggaagccac gcggcagttt cagtccccgc
     8161 tgcgcgcgct tcccgatgta cggctcgaga tccgcgccct tcaggttcgt gtgccgcttg
     8221 cccgcctgca cgaccagcgt ccctgcctcg ccgaccaccg ccgtgccgac caccttctcc
     8281 gggaacagct ctgttttgtg cagcttgatc gtctgcacgc ccttgccgcg
//
LOCUS       DATLVW010000422         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_62197, whole genome shotgun sequence.
ACCESSION   DATLVW010000422 DATLVW010000000
VERSION     DATLVW010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62197"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1404)
                     /locus_tag="VNN25_12710"
     CDS             complement(58..1404)
                     /locus_tag="VNN25_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH92435.1"
                     /translation="MSAVLSSLSKPKPKRSRKKLYWIIGIILVIAIAGVAVAMSKKRE
                     KPILVTTDKAFRKTITQLVTATGKIQPEIEVKISPEVSGEIIALPVKEGQIVHKGDLL
                     LRIKPDTYRAQVEEQQAALSSARAASATQKAQLAKAQADYQRSQHLFEARLIPESDRK
                     AAQTNYEIAQSALNASQFDIQRSEGALRQINEALSKTTIVAPSDGSISSLSSRLGERV
                     VGTSQFAGTEVMRIADLTSMEARVNVNENDIVNVKVGDTARISVDAYPDRKIAGVVRE
                     IASTATTNNAGTQEQVTNFEVKIRVNDPTVHLRPGMSTTADIETDTVQNAIVVPIQSV
                     TVRTTDSKLSPEEREKQTSQSVSKEDDNKAEVSNQTLEKQKAREQREKLVRVVFIKNG
                     DTVRQQKVETGIADTTFIEIKNGIKVGDEVVSGSYTAISRKLKDGAKVSIEKQAKS"
     gene            complement(1620..>2279)
                     /locus_tag="VNN25_12715"
     CDS             complement(1620..>2279)
                     /locus_tag="VNN25_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92436.1"
                     /translation="ANCSAGAHAGVDPDDNLPGSGRVLDISTASPVVLPDGSIVYGSY
                     TRYNDRNGHLMHFSANGAYLGAYPFGWDTTPAVWQHDGTYSLVTKQNFYGDPLLRTDL
                     GHFMIQLNPQLQVEWQFKNPSRSVCRGGTCADEKFGFEWCVNATAIDAQGTVYANGED
                     GILYAIAQGGIVLDRFSLSAPLGAAYTPLAIDAQGRIYAQSGGHLFAVGGVPARKRAV
                     R"
BASE COUNT          350 a          667 c          732 g          530 t
ORIGIN      
        1 cagagacgag gggcggcttt cctctgatct cttgtctcta atctctgata tctcgaatta
       61 actcttcgcc tgcttctcaa tcgatacctt cgctccatcc ttcagcttgc ggctgatggc
      121 ggtgtagctg ccggagacga cttcgtcgcc gaccttgatg ccgttcttga tctcgatgaa
      181 cgtcgtgtcg gcgattccgg tctcaacttt ctgttggcgc acggtgtcgc cgttcttgat
      241 gaagacgacg cgcacgagct tctcgcgctg ctcgcgcgcc ttctgcttct cgagagtctg
      301 gttactcact tcggctttgt tgtcgtcttc tttggagacg ctttgcgaag tttgtttctc
      361 gcgctcctcg ggcgacagtt tgctatccgt agtgcggaca gtgacgctct ggatcggcac
      421 gacgatcgcg ttctgcaccg tgtcggtctc gatgtcggcg gtcgtgctca tgccgggacg
      481 gagatgcacg gttggatcgt tgacgcggat cttcacttcg aaattcgtca cttgctcctg
      541 cgtgcccgcg ttgttcgtgg ttgcggtgct ggcgatctcg cggacgacgc cggcgatctt
      601 gcggtcggga taggcgtcga cgctgatgcg cgccgtgtca ccgactttca cgttgacgat
      661 gtcgttctcg ttgacgttga cgcgcgcctc catgctggtg aggtcggcga tgcgcatgac
      721 ttccgtgcct gcgaactgac tggtgccgac gacgcgctcg ccgaggcggc tggagagtga
      781 gctgatggag ccgtcgctcg gcgcgacgat ggtcgtcttc gacaacgcct cattgatctg
      841 gcggagtgca ccttcggagc gttggatatc gaactgcgac gcgttgagcg ccgactgcgc
      901 gatctcatag ttcgtctgcg cagccttccg gtcggactcg gggatcagcc gcgcttcgaa
      961 aagatgctgc gaccgctgat agtcggcctg cgctttcgcg agctgcgctt tctgcgtcgc
     1021 gctcgcggcg cgggcgctgc tcagcgccgc ctgttgctct tcgacctgcg cgcggtacgt
     1081 gtcgggcttg atgcgcagca gaaggtcgcc cttgtgcacg atctgccctt ccttgaccgg
     1141 cagcgcgatg atctctcccg agacctcggg cgagattttc acttcgatct ccggctggat
     1201 cttgccggtt gccgtaacga gctgcgtgat cgttttccgg aacgccttgt cggtggtgac
     1261 gaggatcggc ttctcgcgct ttttcgacat tgccacggca acgccggcga tggcgatgac
     1321 gagaatgatg ccgatgatcc agtagagctt cttgcgcgag cgtttcggct tcggcttgct
     1381 gagtgacgat aagacagcgc tcatgtgact ttcaactcca gacgggcatc agccctggtg
     1441 attggactgc gtgatgtcaa cggattcgcg acaggaaagt tgcagacttg gacttttttt
     1501 ggccaacagc ccggagttgc ggcctattct gcggaagtcc gccacgcaca gagacgctga
     1561 ggccgcaggg aaggtttttc tctgtgtcct cagtgtctct gtggttcatt cctgtccgac
     1621 tagcgaacgg cccgcttccg cgcagggacg cctccgaccg cgaacaggtg gccgccggat
     1681 tgtgcgtaga tgcgtccctg cgcatcgatg gcaaggggcg tataggcggc gcccagcggt
     1741 gcggagaggg agaagcggtc gaggacgatg ccgccctggg cgatcgcgta aaggattcca
     1801 tcttcgccgt ttgcgtagac ggtgccctgc gcatcgatgg ccgtggcgtt gacgcaccac
     1861 tcgaagccga acttctcgtc ggcgcaagtg ccgccccggc agaccgaccg cgatggattc
     1921 ttgaattgcc actccacttg caactgcggg ttgagctgga tcatgaaatg gcccagatcc
     1981 gtccgcagca gaggatcgcc gtagaagttc tgcttcgtca ccagcgagta ggttccgtcg
     2041 tgctgccaga ccgccggcgt cgtgtcccag ccgaatgggt aggcgccgag gtacgcgccg
     2101 ttggcggaga aatgcatcag gtggccgttc cggtcgttgt agcgggtgta tgagccgtag
     2161 acaatggatc cgtcggggag aacgaccggc gaggcggtcg agatgtccag gacgcggccc
     2221 gaccccggca gattgtcatc gggatcgact ccggcatgcg cgcccgcgct gcagtttgc
//
LOCUS       DATLVW010000423         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_267497, whole genome shotgun sequence.
ACCESSION   DATLVW010000423 DATLVW010000000
VERSION     DATLVW010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267497"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VNN25_12720"
     CDS             <1..215
                     /locus_tag="VNN25_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009034392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HXH92437.1"
                     /translation="LYYGTREEIEHLRPRSEPVNTFQFGEQTITTKVNMMVPRDAGVQ
                     HIDVLSAMEESPVGSAAMLDSVFDDM"
     gene            229..786
                     /locus_tag="VNN25_12725"
     CDS             229..786
                     /locus_tag="VNN25_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXH92438.1"
                     /translation="MTTTPTRTTPDQLSIPPDGRPYHDQPQWRNDFPIDWPQDELVAR
                     RDFTKFLVLTSFAFVVGQLWIVIENFARKRKGKPPVRRVARADAIAVGSSLVFNYPEA
                     HDICVVVRTGAQQYFAYNQKCTHLSCAVVPDVANGRFHCPCHNGSFELATGVPIAGPP
                     RRALERILLEIRGDDLYAVGTEVRA"
     gene            783..1010
                     /locus_tag="VNN25_12730"
     CDS             783..1010
                     /locus_tag="VNN25_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HXH92439.1"
                     /translation="MKRAVSRQQKMTIVFGILSIVVIIAVLQLWLLTATMNAFLAGQE
                     RVIIPAAVASLICFAFNAGLLSYIYRLERRQ"
     gene            1007..2050
                     /locus_tag="VNN25_12735"
     CDS             1007..2050
                     /locus_tag="VNN25_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HXH92440.1"
                     /translation="MTAASGETIRTLHPAYFALVMATGIVSIAAQLLHVPFIPQLLLG
                     INLLAFAVLWALTIVRIVRYPRDVIADIADHQRGVGFFTAVAATSVLGSQLVIVANGE
                     RFGFAFWIATLVLWVVLVYAVFTALTVKEVKPTLDQGINGGWLVAVVATQSVSTLASI
                     LAPSTSHAELLLFVSLSFWLAGGMLYIWLISLIFYRYTFFRFAPSDLMPPYWINMGAM
                     AISTLAGTLLIANAKFSPLLTMLHPFTLGFTILFWATATWWIPMLLILGFWRHVYKRF
                     PLTYSPLYWGAVFPLGMYTVCTFRLANVAGIPMLQLIPRVFVVIALAAWSLTFIGLLL
                     SLLRSIRTSPAAR"
     gene            2147..>2269
                     /locus_tag="VNN25_12740"
     CDS             2147..>2269
                     /locus_tag="VNN25_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HXH92441.1"
                     /translation="MTTTFDVAGQTVGDLAAAMPLSIRVFEAWKIDYCCGGKTPL"
BASE COUNT          371 a          748 c          665 g          485 t
ORIGIN      
        1 cgctttacta cgggacgcgc gaagagattg agcaccttcg cccacgctcc gagccggtca
       61 acacgttcca gtttggtgag cagaccatca ccacgaaggt gaacatgatg gtgccgcgcg
      121 acgcgggtgt gcagcacatc gacgtccttt ccgcgatgga ggagagcccg gtcggaagcg
      181 cggccatgct cgattccgtc ttcgacgaca tgtgagggct tgattcgcat gaccacgaca
      241 cccacgcgca cgacacctga tcagctctcc attccgcccg atggtcggcc gtaccacgat
      301 cagccacagt ggcgaaacga ctttccgatc gactggccgc aggacgagct cgtggcccgc
      361 cgcgatttca ccaagtttct cgtgctgacg agcttcgcgt tcgtcgtcgg ccaactctgg
      421 atcgtcatcg agaacttcgc ccgcaaacga aaaggaaagc cgccggtgag gcgcgtggcg
      481 cgcgcggatg cgattgccgt cggcagctcg ctcgttttca actatcccga ggcgcatgac
      541 atctgcgtcg tggtgcggac cggcgcgcag cagtacttcg catacaacca gaagtgcacg
      601 cacctgagct gcgcggtcgt tccggacgtc gccaacggcc ggttccactg cccgtgtcac
      661 aacggttcct ttgaactggc cacaggggta ccgattgccg ggccgccgcg acgtgcactc
      721 gagcgcatcc tgctcgagat tcgcggcgac gatctctacg cggtgggaac cgaggtgcgg
      781 gcatgaagcg cgcggtctcg cggcagcaga agatgacgat cgtcttcggc atcctctcga
      841 tcgtcgtcat catcgcggtg ttgcagctct ggctgctgac cgccacgatg aacgcgttcc
      901 tggccggtca ggagcgggtg atcattcccg cggcagtggc gagcctcatc tgttttgcgt
      961 tcaacgcggg cctgctttcc tacatttatc gcttggagcg ccggcagtga ccgcggcctc
     1021 gggcgagacc atccgcacat tgcatccggc gtatttcgca ctcgtcatgg caaccggaat
     1081 cgtctcgatt gccgcccaac tgctccacgt ccctttcatc ccccaacttc tcctcggcat
     1141 caatcttctc gccttcgccg ttctctgggc gctgacgatc gtgcggatcg ttcgctaccc
     1201 gcgagacgtg atcgcggata ttgcggacca tcagcgcggc gttggctttt tcaccgccgt
     1261 ggcagcgacg agcgtcctcg gatcgcagct cgtgatcgtg gcgaacggag agcggttcgg
     1321 attcgcgttc tggatcgcca cgctcgttct ctgggtcgtg ctggtctacg cagtcttcac
     1381 cgcgctcacg gtcaaggaag tgaagccgac gctcgatcag ggcatcaacg gtggctggct
     1441 cgttgccgta gtggctacgc agtcggtgtc gacgctcgcg tcgattctcg cgccttcgac
     1501 gtcgcacgcg gagctgctgc tgttcgtgag cctctcgttc tggctcgccg gcggcatgct
     1561 ctacatctgg ctcatctcgc tcatctttta tcgctacacg ttcttccgct tcgcgccgtc
     1621 cgacctgatg ccgccgtact ggatcaacat gggagcgatg gccatctcga cgctggccgg
     1681 cacgctgctg atcgcgaacg cgaagttctc gccgctgctc accatgctcc atcccttcac
     1741 gcttggcttc acgatccttt tctgggccac cgccacctgg tggataccga tgctgctgat
     1801 ccttggattc tggcgccacg tctacaagcg cttcccgctg acgtacagcc cgctttactg
     1861 gggcgcggtc ttcccgctcg gcatgtacac ggtctgcacg ttccggctgg cgaacgtggc
     1921 cgggattccg atgctgcagc tcatcccgag ggtcttcgtg gtcatcgcac tcgcggcgtg
     1981 gtccctcacg ttcatcggct tgctgctgag tctcctccga agcatacgga cctcgcccgc
     2041 cgcgcgctga cgcatcgctg cgacacgatc tcaattccgt atgacatccg tcatgtgctc
     2101 ggcccttgtg cggcacgaca atgtgctcat gacggaggaa acaaccatga ccacgacctt
     2161 tgacgtagct ggacaaaccg taggcgacct tgctgctgcg atgccgctct cgatccgcgt
     2221 gtttgaagcc tggaagattg actactgctg cggaggcaaa accccgctg
//
LOCUS       DATLVW010000424        22812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_100331, whole genome shotgun sequence.
ACCESSION   DATLVW010000424 DATLVW010000000
VERSION     DATLVW010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22812
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100331"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VNN25_12745"
     CDS             complement(<1..567)
                     /locus_tag="VNN25_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HXH92442.1"
                     /translation="MINKVVASADEAVRDVADGATLVVGGFGLCGIPENLINALVKRG
                     VKGLTCVSNNAGVDDWGLGLLLQTRQIRKMVSSYVGENAEFERQFLAGELEVEFVPQG
                     TLAERMRAGGAGIPAFFTPAGYGTIVAEGKETREFDGRMYVMERGIVGDFSLVAAQKA
                     DRLGNLQYRKTARNFNPMAATAGKICIAE"
     gene            805..3216
                     /locus_tag="VNN25_12750"
     CDS             805..3216
                     /locus_tag="VNN25_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92443.1"
                     /translation="MTSEPFGYRADDVHPFWMRLLREVGAFAFFLLFTVILIWPVASH
                     LNTAVPDLGDPLLNTWILDWDLYSITNAPTHIYDANIFYPSKYPLAYSENLFGIAIVA
                     LPFYLLGFTPLAVYNIAILLGFAFCGYGAYVLGRVLTRSVPAGLVAGILYSFVNFRTD
                     HLAHLQFVWGGWMPMMLAALLHYWRRPTTRNAILFGLCVLMNGLTNIHQFLFGTTAIV
                     LSIALLALLTTRFKWRTLIGAALATILAAALMVPVLLPYKEVSELYGMVRDRNTAMWG
                     SAIWTDWLSANVRNRAYGDIADPANVRPEAVLFPGLMMLFLTASALLIDRRKLPVALD
                     ARPVPRWLLHLLDAIIVAMAIGTYIGAATKNYAWRLLGHTIVAFHTTSKPASSDVPLV
                     ILIVMVMVRLSLQLPKGLSGDTLQSLRTAAARSRMPIEIWVMLLWIVLGVLGSLGLHT
                     FVHTFLYDHLTMFRSMRVPARWATDAYVGLAGASACGAAAWIERRRTKIARWAIAGLL
                     IALAIHDVLPIIRWEHALEITDPAYVWMRDTKYSGGTLEMPVSEGIAQYLYQFGATIH
                     HKPLMNGTSGFEPPLHAKIAGMASKKVIPDELLPLLAHNDCHFLLVHDDWLRGASEPT
                     HLWLTRELALGRIGFVRRFDHRGGGDYIFAVTSNVPNWRGLRPPESGDAAGFTEELEM
                     QRALRGEATYMNHTFGVVDSPRYDSTVTGPMVVNGWALSPNGVAAVDVILHNGKYRYR
                     AVPTVRYDVQAKYPWYPKDKHGGFTLTFPKRPRGVPQWTDAQIEVIDGRGGRTKFPDI
                     VLNWQ"
     gene            3365..5758
                     /locus_tag="VNN25_12755"
     CDS             3365..5758
                     /locus_tag="VNN25_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92444.1"
                     /translation="MSPEQIGYRAESAHPLGVRVLQEVGAFVFFLLFTIVLTWPVAAR
                     LDTAVAYLGDPLLNTWILDWDLHAITSAPSHLYKANIFYPSKDPLAYSENLFGIAIVS
                     FPFYLLGFTPLAIYNVALLLGYAFSGYGAYVLGRTLTRSAAAGLVAGILYAFVSFRVD
                     YFALLQFVWGGWMPLMLAALIHYWRRPVARNAVLYGLCVLMNGLTNIHQFLFGTTALV
                     LSVALLALLSVRFRWRTLMGAGLATILAAALMVPVLLPYKRVSDLYGMVRDRNAAMAG
                     SATWNDWLSSSSKNRAYGDVSDREKVLPERALFPGLMMLFLTASALLLDRSKRPVPLD
                     ARPVPRWLLLLLDTVIVVFSVATYIGTATLHYEWTLFGRTLIAIDSAGTPIVCLVIAI
                     VARLSLRLPNARSAEHAQSLRTLAARSRMPVEIWVMLLWIVLGVLGSLGLHAFLHSFL
                     FDHFLPFRSIRITSRWASIAYVGLAGSSACGAAAWINARRSKVARWGVAALLIVLSIH
                     DVLPMFRWEHALDAADPAYVWMHDTKFSGGTLEIPVSEGIAPYLYQFGATIHHKPLMN
                     GTSGFEPPLHAKIREMAAMKTIPDDLLPLLVHNDCHFLLVHDDWLRGASDSTHAWLGR
                     ELGLGRINFVRRFDHRGGGDYLFAVTANVPNWHELRPPDKRDGASFTDDQELQRALRG
                     EATYMGRTFGIVDTPHAEQNVTGPLTISGWALAPNGITEVDVMLDNDTHRYRATPTDR
                     YDVKMKFPWYPRVRHAGFTLTLPKRPKGVPRWTDVQVEIIDGHGGKTKLPDVVLNWD"
     gene            complement(5805..6143)
                     /locus_tag="VNN25_12760"
     CDS             complement(5805..6143)
                     /locus_tag="VNN25_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92445.1"
                     /translation="MRNVLDKRDQNLVNINQILVINSQILVDNSQILVINSQVLVDNN
                     QILVINSQVLVDNSQILVDDVQILDNVTLKAIRRCRRWRFQNGVRSKFESDETRQGVA
                     DRFCHFQIQT"
     gene            6233..8806
                     /locus_tag="VNN25_12765"
     CDS             6233..8806
                     /locus_tag="VNN25_12765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH92446.1"
                     /translation="MQTPAPDETVRLNETTPRSSLPYAQFAPGSIIAGRYRIVSLLGS
                     GGMGEVYRADDLKLGQQVALKFLPAVFARDATQLALLHDEVRLGRQVAHPNVCRVYDI
                     GEWDGAHFVAMEYVDGEDLARLLQRIGRLAHDKAVEIARGVAAGLSAAHAKGILHRDL
                     KPANIMIDGRGEPRITDFGLALASEDAVDSNIVAGTPAYMAPEQLEGKAATVQSDLYA
                     LGLVMYEIFTGRRARSGRTIPDLRREHATQITTPSNVVRDIEPAVERIILRCLESDPS
                     QRPRSARQVFEALPGGDPLAAAIAAGETPSPDLVASAGIEGTLSPAAAWGWVGAIAVM
                     LACILGITSDWRIMSKIPFEKPPEVLEQNAIDIGRAIGIEPGKYRVRAFEAQSPYLAW
                     LSEHDRSPKRFDHLRSGPPSVVLRLRESATPLTTLSDNTAASKDDPPLREGMVTIEVD
                     TNGRLFGLAAPRGHATTAHADWSKVLRLAGFDSATLTSVTPRIVPPFFADERVAWNGF
                     WPDDRTKPAHIEAAAAGGVPVFFKITGPWNDDPSAVGRQPFETPSLAIFETILFTSLT
                     LATIVLAWRNLRTRRGDRSGASHVAAAVFAIECSARLLAADHRAVFAHESSVIISAVR
                     MALLWAVAYYFLYVALEPYVRRRWPERLIAWSRLIGGNIRDPLVGRDLLIGIAAGLAH
                     VFIATATPWLPGRLGWRAPGTPATWAVNTLLGVRVAISEVASQISSGIITGLAMVVLL
                     VVLAIVLRRRSLAAAGFYLLLLIASAFASGGNPYIFGGTVVIAAIWTAVVVRVGLLGI
                     VTAQTIFSVAFFLPMSVDFSAWYLPSAIAPVIFIAILTAVAFRIAIGNQPMWSGKLLD
                     E"
     gene            complement(8818..9429)
                     /locus_tag="VNN25_12770"
     CDS             complement(8818..9429)
                     /locus_tag="VNN25_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HXH92447.1"
                     /translation="MVQHAAFAEHVALLTACAACPNVFGRPVVGAVAGARVMLVGQAP
                     GPHEVDARRPFAYTAGKRLFAWMAGIGVDEETFRDRVHIAAVIRCFPGKDAKSGGDRV
                     PDAEEIARCGAHLDREISILKPKLVIAVGTLASQQMIGIAQLKDAVGLLHRARRAGRA
                     FDVVVLPHPSGRSTWLNKPENLALLNESLRLIAEHPAFVGAFR"
     gene            9441..10121
                     /locus_tag="VNN25_12775"
     CDS             9441..10121
                     /locus_tag="VNN25_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92448.1"
                     /translation="MKRLAAALLFLSIALPSMAWTRAADLRIAEKSAALAPPDLRLLL
                     DKYHDEYLHGVDDALSSEGTDIHRRRLRERIEAQTRGIVQMIRTNQPMSAVVAQLGNL
                     SHLVGDANNPFHIGDDDAAAHADFEQYFEHRLARFSPVFYGVDPNFTLNHYLDKVFTR
                     TTNLGPLMTEEYKNGDGSTFDDRSTAFGVASVCYSHAITDTVNLYYYIWKSAGGSTRP
                     MRGGFNAH"
     gene            10111..11613
                     /gene="purH"
                     /locus_tag="VNN25_12780"
     CDS             10111..11613
                     /gene="purH"
                     /locus_tag="VNN25_12780"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXH92449.1"
                     /translation="MRIESIARAILSVSDKTGIVELARALRAKNVEILSTGGTAKHLA
                     DNCIEVTSIAQWSGAPEILGGRVKTLTPKVFGAILHDRDNAGHRDDVNRLGIPPIDLV
                     VVNLYPFESTIAKEGVTIAEAIEQIDIGGPSMLRAAAKNHKHVLPLCDPSLYADFIRE
                     FENREISETFRLRCAIEVFRKTAAYDAMIAKFLSSTSDTLTLNLTKFQDLRYGENPQQ
                     TAAFYVPQGTTPFEQIQGKELSYNNLLDLDSAMRLAQAFHEPAAAIIKHTNPCGVARR
                     DSLVDALKAAIESDPVSAFGGIIGANREFDADAAAVVESMFLEVIVAPSFSDKAREIL
                     AKKKNLRLIVAQPLTNDTDYRSAAGGILAQSRDRIGSRDGWKVVTERHPTPEEMDGLE
                     FAWIVCAHVKSNAIVLTNQSQTVGIGAGQMSRVDAAKVAIMKSILPITGTVAASDAFF
                     PFRDGLDLLADANIRAIIQPGGSVRDAEVIAAANERGIAMVFTGERHFRH"
     gene            11796..13361
                     /locus_tag="VNN25_12785"
     CDS             11796..13361
                     /locus_tag="VNN25_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92450.1"
                     /translation="MVKRDSDRGMFAEPFGQADCESELETLRERYSEFYFEHSPFNEA
                     ALNEKVYLIVGRRGSGKTALSEYFSFQKRIPNPVCIEIHEPSVYQEVLAALASRAPEA
                     RVVAVPALTKVWEFVIWSTIFHSLKTENVDIQLACEVPTSRLGRVSDVVKTFFEHLTS
                     VFHDGHRGNQASGDFSDLYDEGQLEANIARVLEFAQERQIIVAMDTLEKYDIREESLM
                     TAMAALVQYASKFNLRYAYRGVHLKLFLSGEVFPYLKETVLLNPLKNIKHPLYLLWRP
                     KELLQLITWRFWRYLTSSSEVYRARLSDVNWSRHKEVFERMWKPFFGSALENRRGRKE
                     RTFVYVLRHTQMRPRQLIFLCNTIANDAILRGDFPTFSPEGIREAVKKAEADLANEIL
                     NSYELTYPSVARIVQALLGMPMIFDGSELDKRAKDTRGEWPDGEYSLANFRQLVAELG
                     IVGRVKDRNDAAGFLDAEFEYSAMERLAISPRDQCAIHPMFYSKLNIKMDQDIRVMPF
                     SVERGELEELQEI"
     gene            complement(13878..14669)
                     /gene="murI"
                     /locus_tag="VNN25_12790"
     CDS             complement(13878..14669)
                     /gene="murI"
                     /locus_tag="VNN25_12790"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXH92451.1"
                     /translation="MGSSQNKTSIGIFDSGVGGLTVFREIARALPHHSLIYLGDSARV
                     PYGTKSAETVTRYSLQAAAHLIDRGIGMLVVACNTATAAALPALQASLKIPVIGVVEP
                     GARAAVARTRGRVGVIATEGTVRSQAYTKAIRLIDPKIEVIESAAPLFVSLAEEGWAN
                     THVAREVAEIYLEPLLDANIDTLVLGCTHYPILRGTIEQVTGDKIAIVDSAETTASFV
                     KNAVRNKPSGTPKHTFLVTDAEERFRRIASDFLEREIEHLELVTL"
     gene            complement(14793..16199)
                     /locus_tag="VNN25_12795"
     CDS             complement(14793..16199)
                     /locus_tag="VNN25_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92452.1"
                     /translation="MPFRILVIDDETAIREAIRMTLEYEGYRIDEARSGQDGIDKATR
                     TAAPYDAILLDIKMPILDGIEVLENLKEQKVPSPVIMVSGHGDISTAVECTKRGAFDF
                     LEKPLNRDKLLLSVRNAVRQQKLEEEVGELREREARDYQLVGDSASMVNLKTQIELAA
                     PTKATVLIQGESGTGKELVAREIHRRSDRKAMPFIQVNCAAIPEELIESELFGHEKGS
                     FTGAVRKQTGKFVAADGGTIFLDEIGDMSLRTQAKVLRVLQEGEVEPVGSATVIKVDV
                     RVIAATNKDLTDEIKSGRFREDLFYRLNVIPVRTPPLRERKDDIPLLAQHFATMFSDQ
                     YNRPAKKFTPSAAKAMQDAAWRGNVRELRNMIERLVIMTPADTIDITDLPAEFFRAAG
                     DIITTTMRLDKLQDFKDEAEKAFILAKLRENGWNVSKTAEAIDTPRSNLYKKIEQYNI
                     KREGGGVMTAPDDLKSEG"
     gene            16471..17931
                     /locus_tag="VNN25_12800"
     CDS             16471..17931
                     /locus_tag="VNN25_12800"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXH92453.1"
                     /translation="MDVTITREPKVAKADHLFVLIAENSKPDVAVAAQAMKAIADAGF
                     TGKADESLTTVAGEPKKITLLGIGKQEALTIRILRTALYSVAKMAKKQRDRNIIIVLP
                     YSVAKLDADATTRIAASALSGSDYKYDTYITKKEDKSLPISAQFVPPEGVDAKRAKQL
                     DLEAKAVAAGIRTVRDLGNAPGNLVTATFIGHRAEEVAKEIGIKCTVYGKREIEKMKM
                     GGLLAVNKGSAEEPRFIVLEYAPRKATKHVALVGKGITFDSGGISIKPSDKMEEMKFD
                     MCGAAAVIGTIQAAAMLALPVRVTGVIASTDNLPSGSAYKPGDIITTMSGKTIEIVNT
                     DAEGRVILSDALHYASELKPDHIIDYATLTGACVVALASEAAGLFSNNDELAQKLIEC
                     GERVGERLWRLPEWDDYKELIRSEWADMKNSGGRWGGATTAAVFLKEFVNCPSWAHLD
                     IAGTAYAEHETSREARGATGAGVRVTVAFLESLVRS"
     gene            17976..18950
                     /locus_tag="VNN25_12805"
     CDS             17976..18950
                     /locus_tag="VNN25_12805"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HXH92454.1"
                     /translation="MRIGPAPRNEVNFATVDSPSLQFIPRQQEAKIWQLDENPIVVPS
                     DHLDAYLGSIDAPQFARYETLDLTIGKYTTLAGDGFATVEIFRFPDFVKAFGAYSMAK
                     SGNHQFLQIPNESFAKAHSIHIWRGPYYVRLTGGGPGNALLQLATFVADRMPPAPNKP
                     AVFAFLPETERVPGSERYSADAAFGQAFLGNSFQATYNVGNDMIEGIVIPASSRQAAA
                     NILDQYKGLYLHNGKLLDPIPNLGEDNFTAEDRFLGRVVAFRIDRFVVAFNGFRERQH
                     LVDLATNADQRILGTIRKQLVTADKQAENGNDGENTSTLPAWLQQKKR"
     gene            complement(19050..20585)
                     /locus_tag="VNN25_12810"
     CDS             complement(19050..20585)
                     /locus_tag="VNN25_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH92455.1"
                     /translation="MSRRIALALIAVCALSVAAQVRENVTVEVVEVPVYITSGNNQPV
                     EGLTRDAFELFVDGRPQPIEYFDAIDFAKVPTPAAQTAVERPRRERRLYLLLFDLSFA
                     TPARIKKAQTAAEQAVARSNPATDFFGVATYSSNRGVQFVSSFLSDRVAITRAIYTLR
                     ASSAQDPLGVALSSPERAQWLTATELGGSAEGISPLLGDQVNEILTGGQANQDMQKMQ
                     GNRLIEYQVDALGDVAARLSALEGQKHILIFTEGFDSTRITDIKANGRPPNLDAHLLD
                     VVKQMHETFVSAGVTLDSIDIQGLRHTLSDLDNDALYMLSRGTGGRVIVNRNDLGDAV
                     DTIMRAERTVYVLGFSSAGRKHGKITVRVNGLPHGSEVSYREGFGYVPPGKNVDALQL
                     ADILLNDVPQSGATLDAGVTSADGTSVLAVAFNRAEVVPQLPNASPGIELLIYILDEH
                     GSTVGFKSKRVSLTGPARVPTGYLTLREPFELPPGKYTAKVLFRIMGTRVLGYVRRDF
                     VVE"
     gene            complement(20582..21061)
                     /gene="moaC"
                     /locus_tag="VNN25_12815"
     CDS             complement(20582..21061)
                     /gene="moaC"
                     /locus_tag="VNN25_12815"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXH92456.1"
                     /translation="MSKNFSHLDETGGISMVDVASKRNTMREAVAVATVRMSPESLEK
                     LVAQALPKGDVLTTAKVAGVLAAKQTPNLIPLAHPLAITSIDITFDLDPAAGTIEVHS
                     IVRCEGKTGVEMEAMTACAVAALTIYDMCKSAEKGITIESLQLMRKSGGKSGTWERS"
     gene            complement(21108..21662)
                     /locus_tag="VNN25_12820"
     CDS             complement(21108..21662)
                     /locus_tag="VNN25_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXH92457.1"
                     /translation="MNAYILIGGESRRMGRSKTELFFDRVAAAAAEVFEDVVAVQRHG
                     GPAVPLTTIYESPHDDQAPVFGVACALAHTSGRCFVIAVDYPLITTAILRFLRDRFER
                     SNALLLAPVWSGKTQMLCAGYDPGLLPRLQERIAAKRYDLRGVAAVGEAEILGEDDLR
                     AQFAGEPLMNVNTLAEWTSLLGSG"
     gene            complement(21659..22327)
                     /locus_tag="VNN25_12825"
     CDS             complement(21659..22327)
                     /locus_tag="VNN25_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXH92458.1"
                     /translation="MAVSVIIPTLNEEERIGATIESAFGAGAAEVIVVDGGSIDRTAR
                     FAKSAGARLLLCEPMRARQLNLGANAAVNDALIFLHADTRLPPGAARAAEDALASGAD
                     FGGFRIAFAEPARKLRAAAALINLRTSITRCPWGDQAQFIRRERFLQTGGFREIPLME
                     DYDLAIRSKRSGRSILLPLTVTTSGRRFLRKGVLRTAVINWRTIMRFRRGADVAELAR
                     SYRR"
     gene            complement(22363..>22812)
                     /locus_tag="VNN25_12830"
     CDS             complement(22363..>22812)
                     /locus_tag="VNN25_12830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92459.1"
                     /translation="EEEYLRALTIFLDTYGAEDASSPLILSKTAKSATGLSYFGLCLM
                     LVQKKTKQAIDLCKRAIELEFYDGNHYANLARVYVAAGNRKKALETVQQAMKDHREDA
                     ALLQVRRELGIRSAPPVPFLDRSNPINVTLGQSRHAKKVAQREGKKK"
BASE COUNT         4185 a         7479 c         6789 g         4359 t
ORIGIN      
        1 ctcggcgata caaatcttcc ccgccgtcgc cgccatcgga ttgaagttgc gcgcggtctt
       61 gcgatactga agattgccca gccggtccgc tttctgcgcg gcgacgagtg agaaatcgcc
      121 gacgatgccg cgctccatca cgtacatccg tccgtcgaac tcgcgcgtct cttttccctc
      181 ggccacaatc gtgccgtagc cggcgggcgt gaagaacgcg ggaatgccgg cgccgcccgc
      241 gcgcatccgc tcggccagcg ttccctgcgg cacgaactcc acttccagct cgccggcgag
      301 gaactggcgc tcgaactctg cgttctcgcc gacgtacgac gagaccatct tgcggatctg
      361 gcgcgtctga agaaggaggc cgaggcccca atcgtcgacg ccggcgttgt tggagacgca
      421 agtgaggccc ttgaccccgc gcttgacgag cgcgttgatc aggttttccg ggatcccgca
      481 gagtccgaag ccgccaacga cgagcgtagc gccatcggcg acgtcgcgga ccgcttcgtc
      541 cgcggatgcg accactttgt tgatcatggg cgcgaagata tcaaacgttc acaaccgccg
      601 gcgggccgcc ggcgatacag ccgccgggcc ggcggcgcta cacgcgcgca cgtcgctcgc
      661 tcgtggcatg ggcaggcgta gcgccgtcgg ctcgacggct gtaccgccac cggctcggtg
      721 gcgactttcg cgttcgcaca acgcgacttg aataacaccg gccttcgtcc cggcaacgcg
      781 acacgtctgc tactctccct atcgatgaca tccgagcctt tcggctatcg cgcggacgac
      841 gtccacccgt tctggatgcg cctccttcgc gaggtgggcg cgttcgcttt cttcctgctc
      901 ttcaccgtca tcttgatctg gcccgtcgcc agccatctca acaccgccgt ccccgacctc
      961 ggcgatcctc ttctcaacac ctggatcctc gattgggatc tctacagcat caccaacgcg
     1021 cccacgcaca tctacgacgc caacatcttc tatcccagca aatacccgct ggcctacagc
     1081 gagaacctct tcggcatcgc catcgttgcg ttgccctttt acctcctcgg attcacgccg
     1141 ctcgccgtct acaacatcgc catcctcctc ggcttcgcct tctgcggtta cggcgcgtac
     1201 gttctcgggc gcgtgctcac gcgttccgtc cccgccggcc tcgtcgccgg catcctctac
     1261 tcgttcgtca acttccggac cgaccatctc gcgcacctgc agttcgtctg gggcggatgg
     1321 atgccgatga tgctcgccgc gttgctgcac tactggcggc ggccaacgac gcgcaacgca
     1381 atcctcttcg ggctctgcgt gctgatgaat ggcctgacga acattcatca gttcctgttc
     1441 ggcacgacgg caatcgtctt atcgattgcg ctactcgcac tgctcacgac gcgattcaag
     1501 tggcgcacgc tgatcggcgc agcgctggcg acgattctcg ccgcggcgct gatggttccc
     1561 gtgctcctgc cgtacaagga agtctccgag ctctacggaa tggttcgcga ccgcaacacc
     1621 gcgatgtggg gctccgcgat ctggaccgat tggctctccg cgaacgtccg gaaccgcgcc
     1681 tacggagata tcgccgatcc ggcgaatgta cgccccgagg cggtgctctt tcctgggctg
     1741 atgatgttgt tcctcactgc cagcgcgttg ctgatcgacc ggcgcaaatt gccggtcgca
     1801 ctcgatgccc gccccgtccc gcgttggctc ctgcatcttc tcgacgccat catcgtggcg
     1861 atggccatcg gcacctacat cggcgcggcg acgaagaact atgcgtggcg gctcctcggg
     1921 cacaccatcg tcgccttcca caccacatcc aaacctgcct cctccgatgt gccgctcgtc
     1981 atcctgatcg tgatggtcat ggtgcggctc tcgctccaac tgccgaaggg tctgtcgggc
     2041 gacacgctgc aatcgctgcg aactgccgca gcgcgctccc gcatgccgat cgagatctgg
     2101 gtgatgctgc tctggatcgt cctcggcgtt ctcggctcgc tcgggctgca caccttcgtg
     2161 cacacctttc tctacgatca cctgacgatg tttcgcagca tgcgcgtgcc ggcgcgctgg
     2221 gccaccgatg cttacgtcgg cctcgccggc gcaagcgcgt gcggcgccgc ggcatggatc
     2281 gaacggcgcc ggacgaagat cgcgcgctgg gcaatcgctg ggctcctcat cgcactggca
     2341 atccacgacg tgttgccgat cattcgctgg gagcacgcgc tcgagatcac cgacccggcg
     2401 tacgtctgga tgcgcgacac gaaatactcc ggcggcacgc tggagatgcc ggtatccgag
     2461 gggatcgcgc agtatctcta tcaattcgga gccacgattc atcacaagcc gctgatgaac
     2521 ggaacctccg gcttcgagcc gccgctgcac gcgaagatcg ccgggatggc cagcaagaag
     2581 gtcattccgg atgagctgtt gcccttgctc gcgcacaacg attgccactt cctgctcgtc
     2641 cacgacgact ggctgcgcgg cgcatcggag ccgacgcacc tctggctcac ccgcgagctc
     2701 gcactcggac gcatcggctt cgtacggcgt ttcgatcatc gcggcggcgg cgactacatc
     2761 ttcgcggtga catcgaacgt cccgaactgg cgcggtctgc gcccgccgga gagtggcgat
     2821 gccgccggct tcaccgagga gttggagatg cagcgcgcgc tgcgggggga ggcgacgtat
     2881 atgaatcaca ccttcggcgt cgtcgactcg ccgcgctacg actcgaccgt cacaggaccg
     2941 atggtcgtca acggctgggc cctctcgccc aacggcgtcg cagccgtcga cgtgattctg
     3001 cacaacggca agtaccgcta ccgcgccgtg cccaccgtgc gctacgacgt gcaggcgaag
     3061 tatccctggt acccgaaaga caagcacggc ggattcacgc tcacgttccc gaaacgcccg
     3121 cgcggcgtgc cgcagtggac cgacgcccag atcgaagtca tcgacggccg cggcggacga
     3181 acgaagttcc cagacatcgt gctgaactgg cagtgagctc gcggtccatg ttcaaaaaag
     3241 cgtttcacgc ggagacgcgg agaacgcaga ggtttttctc cgtgtccccc gcgtctccgc
     3301 gtgagacgct ttttggacag ccccaccttg cgcccccgtc acctcaccgc tactctcccg
     3361 tccggtgtcg ccagaacaaa tcggctatcg cgcggagagc gcgcatccgt tgggagtgcg
     3421 cgtgctccag gaggtcggtg cgttcgtctt ctttctcctg ttcacgatcg ttctgacgtg
     3481 gcccgtcgcc gcgcgtctcg acaccgctgt cgcctacctc ggcgatccgc tactgaacac
     3541 atggatcctc gactgggatc tgcacgccat caccagcgcg ccatcgcatc tctacaaagc
     3601 caacatcttc taccccagca aggatccgct ggcgtacagc gagaacctct tcggcatcgc
     3661 catcgtctcg tttccgttct atctcctcgg cttcacgccg cttgctatct acaacgtcgc
     3721 gctcctcctc ggctacgcct tcagcggcta cggtgcctac gtcctcgggc gcacgctgac
     3781 gcgctcggcg gccgcggggc tcgtggccgg catcctttac gccttcgtca gctttcgcgt
     3841 cgactacttc gccctgctgc aattcgtctg gggcggatgg atgccgctga tgctcgccgc
     3901 gctcattcac tactggcggc gtccggtcgc gcgcaatgcg gtgctgtacg gactctgcgt
     3961 gctgatgaac ggcctcacga acattcatca gttcctcttc ggcacgaccg cgctcgtcct
     4021 gtcggtggcc ctgctcgcgc tgctctccgt gcgctttcgc tggcgcacgt tgatgggcgc
     4081 gggcctggcg acgattcttg ccgcggcgct gatggtgccg gtgctgctgc catacaaacg
     4141 cgtctccgac ttgtacggga tggtgcgcga ccgcaacgca gcgatggcag gctccgcgac
     4201 ctggaacgac tggctctcct cgagcagcaa gaaccgcgcg tacggtgatg tctccgaccg
     4261 cgagaaggtc ctcccggaaa gggcgctctt ccccggactg atgatgctct tcctcaccgc
     4321 cagcgcgttg ctgctcgatc gtagcaaacg tccggtgccg ctcgatgcgc gtcccgtccc
     4381 tcgctggcta ctgcttctgc tcgacacggt gatcgtcgtc ttctccgtcg ccacgtacat
     4441 cggcacggcc acgcttcact acgaatggac actcttcggc cgcacgctca tcgcgatcga
     4501 ttccgccggc acgccgatcg tctgcctcgt catcgcgatc gtggctcgcc tctcgctgcg
     4561 attgcccaac gcacgatcgg cggaacacgc gcaatcgctg cgcacgctcg cggcccgctc
     4621 gcggatgccg gtcgagatct gggtgatgct cctctggatc gtcctcggcg tcctcggctc
     4681 gctggggctt cacgctttcc tgcactcttt tctgttcgat cactttctcc cgttccgcag
     4741 cattcgcatc acgtcacgct gggccagcat cgcttacgtc ggactggctg gctcgagcgc
     4801 gtgcggcgcg gcggcgtgga tcaacgcgcg ccggtcgaag gtcgcgcgct ggggcgttgc
     4861 cgcgcttctc atcgtgctgt cgattcacga cgtcctgccg atgttccgct gggagcatgc
     4921 gctcgatgcc gccgaccccg cctacgtctg gatgcacgac acgaagtttt ccggaggcac
     4981 gctggagatc ccggtgtcag agggcatcgc gccgtacctc taccagttcg gcgcgacgat
     5041 tcatcacaag ccgttgatga acggaacgtc cggcttcgaa ccgccgctgc acgcgaagat
     5101 cagggaaatg gcggcgatga aaaccattcc tgacgatctg ctcccgctgc tcgtgcacaa
     5161 cgattgccac ttcctcctcg tgcacgacga ttggctgcgc ggcgcctccg attcgaccca
     5221 tgcctggctc ggccgcgagc tcggcctcgg gcgcatcaac ttcgtccggc ggttcgatca
     5281 ccgaggagga ggcgactacc tcttcgcagt cacggccaat gttcccaact ggcacgagct
     5341 gcgtccgccc gacaaacgcg acggcgccag tttcaccgac gatcaggagc tccagcgcgc
     5401 ccttcgcggc gaggccacct acatgggccg cacattcggc atcgtcgaca caccacacgc
     5461 cgaacagaac gtcaccggcc cgctcacgat aagcggctgg gccctcgcgc cgaacgggat
     5521 caccgaggtc gatgtgatgc tcgacaacga cacgcatcgc tatcgcgcca cacccaccga
     5581 ccgctacgat gtgaaaatga agttcccctg gtacccccgc gtacgccatg ccggattcac
     5641 gctaaccctg ccgaagcgcc cgaaaggcgt gccgcgctgg acggacgtgc aggtagagat
     5701 catcgacggc cacggcggaa agacgaagct gccggacgtt gtactgaact gggattgagg
     5761 gccgtctcgc cccctcattc gcacacgccg ctttcaaaat ggggtcaggt ctgaatctga
     5821 aagtgacaaa acctgtcggc tacaccctgt cgtgtttcgt cactttcaaa tttggacctg
     5881 accccatttt gaaagcgcca tctgcgacat ctgcggatcg cctttagggt aacgttgtcc
     5941 agaatctgga cgtcatcaac cagaatctgg ctgttatcaa ccagaacttg gctgttgatg
     6001 accagaatct ggttgttatc aaccagaacc tggctgttga tgaccagaat ctggctgtta
     6061 tcaaccagaa tctggctgtt gataaccaga atctggttga tgttgaccag attctggtct
     6121 ctcttatcga ggacgttcct caccttgccg gagtcgaggc gcgctttaac aaggatgcgc
     6181 ttgctgaagc aattggcgct ccctcccccg cgtacaatgt tcccttccaa acatgcaaac
     6241 gcccgcgccg gacgaaactg tccgccttaa cgaaacgacg ccgcgttcgt cgctgccgta
     6301 cgcgcaattt gcgcccggct cgatcatcgc cggcaggtat cgcatcgtca gccttctcgg
     6361 ctccggcggc atgggtgagg tttaccgcgc ggatgatctg aaactcggcc agcaggtggc
     6421 gctgaagttc cttcctgcgg tgtttgcacg cgacgccaca caactcgctc tcctccacga
     6481 cgaggtccgc ctcggccgcc aggtcgcgca tccgaacgtc tgccgcgtct acgacatcgg
     6541 cgaatgggat ggtgcgcatt tcgtggccat ggagtacgtc gacggcgagg atctggcgcg
     6601 gctgctccag cgcatcggac gcctcgcgca tgacaaagcg gtggagatcg cgcgcggcgt
     6661 cgctgccggc ctttccgcgg cgcacgcgaa aggcatcctt catcgcgacc tcaagccggc
     6721 caacatcatg atcgacggcc gcggcgagcc gcgcatcacc gacttcggcc tcgcgctcgc
     6781 cagcgaggat gccgtcgaca gcaacatcgt cgccggcacg cccgcctaca tggcgcccga
     6841 gcagctcgaa ggaaaagcgg caacggtgca gagtgacctg tacgcgctcg gcctggtcat
     6901 gtacgagatc ttcaccggcc gccgcgcccg cagcggacgc accattccag acctgcgccg
     6961 cgagcacgcc acccagatca cgacgccgtc gaatgtcgtg cgcgacatcg agccggctgt
     7021 cgagcgcatc atcctgcgct gcctcgaaag cgatccatcg cagcgtccac gatcggcgcg
     7081 gcaggtcttc gaagcgctcc ccggcggcga tccgctcgct gccgcaattg ccgccggcga
     7141 gacaccgtcg cccgatcttg tcgcctctgc cggcatcgaa ggaacgctct ctcccgcagc
     7201 ggcctggggc tgggtcggag cgatcgccgt gatgctcgcc tgcatcctcg gcatcacctc
     7261 cgactggcgg atcatgtcga agattccgtt cgagaagccg cccgaggtgc tcgagcagaa
     7321 cgccatcgac atcggccgcg cgatcggcat cgagccggga aaatatcgcg tgcgcgcatt
     7381 cgaagcgcag tctccgtatc tcgcctggct gagcgaacac gatcgctcgc cgaagcgctt
     7441 cgatcacctg cgcagcggcc cgccctccgt cgttcttcgg ctccgcgaaa gtgcgacgcc
     7501 gctgacgacg ctttccgaca acaccgcagc atcgaaggat gacccgccgc tccgcgaagg
     7561 catggtcacg atcgaagtcg ataccaacgg ccggctcttc ggactggcag cccctcgggg
     7621 gcacgcgaca acagcgcacg ccgattggag caaagtgctt cgcctggccg gcttcgattc
     7681 cgcaacgctt accagcgtca cgccgcgcat cgttcctccg ttcttcgccg acgagcgcgt
     7741 ggcatggaac gggttctggc ccgatgatcg aaccaagccg gctcacatcg aagcggctgc
     7801 cgcgggcggc gtgcccgtct tcttcaaaat caccggaccg tggaacgacg atccaagcgc
     7861 agtcggacgc cagcccttcg aaacgccttc cctggcgatc ttcgaaacaa tcctcttcac
     7921 ctcactcacg ctcgccacga tcgtgcttgc gtggcgcaac cttcggacgc gccggggaga
     7981 tcgcagtgga gcgtcgcatg tcgcggccgc tgtcttcgcg atcgagtgca gcgccagact
     8041 actggccgcc gatcatcgcg ccgtctttgc ccacgagagt agcgtcataa tttccgcggt
     8101 ccggatggct ctcctctggg ccgtggccta ctattttctc tacgtcgcgc tcgagccgta
     8161 cgtgcggcga cgctggccgg agcgcctcat cgcttggtcg cgtctgatcg gcggcaacat
     8221 tcgcgatccc ctcgtcggac gcgatctgct catcggcatc gccgccggtc tcgcgcatgt
     8281 gttcatcgcc acggccacac cgtggctgcc aggacggctc ggctggagag cgcccggaac
     8341 tccggcgaca tgggctgtga acaccctgct tggcgtgcgc gtcgccattt ccgaggttgc
     8401 ctcgcagatc agcagcggca tcatcaccgg cctggcgatg gtcgttctgc tcgtcgtgct
     8461 ggccatcgtg ctgcgccgcc gcagccttgc tgccgccggg ttctaccttc tgctgctcat
     8521 cgcgtccgcc ttcgcctccg ggggcaaccc gtacatcttc ggcggcacgg ttgtcatcgc
     8581 cgcgatctgg accgccgtcg tcgttcgcgt cggcctcctc ggaatcgtca ccgcacagac
     8641 gatcttcagc gtcgcattct tcctgccgat gtccgtcgac ttctccgcct ggtatctgcc
     8701 gtccgcgatc gctccggtca tcttcatcgc catcctcacc gccgtcgcct tccgcatcgc
     8761 catcggcaac caaccgatgt ggagcggcaa actcctcgac gaatagtttc aacccgatca
     8821 gcggaaggcg cccacaaacg cgggatgctc cgcaatcaac cgcagcgact cattcaacaa
     8881 tgccagattc tccggcttgt tcaaccaggt cgaccgcccc gacggatgcg gcaacacgac
     8941 cacatcgaac gctcgacccg cgcgacgagc gcgatgaagc aaccccaccg catccttgag
     9001 ctgcgcgatc ccgatcatct gctgcgaggc cagcgtcccc accgcgatca ccagcttcgg
     9061 cttcagaatc gaaatctcgc gatcgagatg cgcgccgcat cgcgcaatct cctcggcgtc
     9121 cggtacgcgg tccccgccgc tcttcgcgtc cttccccgga aaacagcgga tcaccgccgc
     9181 gatgtgaacg cgatcgcgaa acgtctcttc gtccacgccg atgccggcca tccatgcaaa
     9241 cagccgcttg cccgccgtgt acgcgaaggg ccgccgcgca tccacctcat gcggccccgg
     9301 cgcctgaccg accagcatca cgcgcgcccc cgccaccgcg ccgactaccg gccgtccgaa
     9361 aacgttcggg caggcagcgc aagccgtcag cagcgctaca tgttccgcaa aagcggcgtg
     9421 ttgtaccatc gcgtccgccc atgaaacgcc tcgccgccgc gctcctcttc ctctcgatcg
     9481 cgctaccgtc aatggcctgg acgcgcgccg ccgatctccg catcgccgaa aaaagcgcgg
     9541 ccctggcgcc gcccgatctg cggctgctcc tcgacaagta ccacgacgaa tacctgcacg
     9601 gtgtcgacga cgccctctcc agcgaaggaa ccgacattca tcgacgccgt ctgcgcgagc
     9661 gcatcgaagc gcagacccga ggaattgtgc agatgatccg caccaaccag ccgatgagcg
     9721 ccgtcgtcgc gcagctcggc aatctctcgc acctcgtcgg cgacgccaac aatcccttcc
     9781 acatcggcga cgacgatgcc gccgcgcacg ccgacttcga gcagtacttc gagcatcgcc
     9841 tggcgcgctt ctcgccggtt ttctacggcg tcgatcccaa tttcacgctc aatcactatc
     9901 tcgacaaggt cttcacacgc acgacgaacc tcggaccact gatgaccgag gagtacaaga
     9961 acggggatgg atcaacgttc gacgatcgct caacagcctt cggcgtcgcc tcggtctgct
    10021 actcccacgc catcacagac accgtcaatc tctactacta catctggaaa tcagcgggcg
    10081 gcagcacgcg gccgatgcgc ggtggcttca atgcgcattg aaagcatagc gcgggcgata
    10141 ctctccgtct cggataagac cgggatcgtc gagctggcgc gcgcacttcg cgcaaagaac
    10201 gtcgagatcc tctccaccgg cggcacagcc aaacacctcg ccgacaattg catcgaagtc
    10261 acctccatcg cacaatggtc cggcgccccc gaaatcctcg gcggccgcgt caagacgctg
    10321 acgccgaagg tattcggcgc catcctccac gaccgcgaca acgctgggca tcgcgacgac
    10381 gtcaaccgcc tcggcatccc gccaatcgat ctggttgtgg tcaacctcta tcccttcgaa
    10441 tcgacgatcg cgaaagaagg cgtgacaatc gctgaggcaa tcgaacagat cgacatcggc
    10501 ggcccgtcga tgctgcgcgc ggcggcaaag aaccacaaac acgtcctccc gctctgcgac
    10561 ccgtcgctct acgcagactt catccgcgaa ttcgaaaatc gcgagatcag cgagacattc
    10621 cgcctgcgct gcgcgatcga agtcttccgc aagaccgccg cgtacgacgc aatgatcgcc
    10681 aagttcctat cttcaacatc cgacacgctc acactaaacc tgacaaagtt ccaggacctg
    10741 cgatacggcg aaaaccccca acagaccgcc gccttttacg tcccgcaagg cacaacgcct
    10801 ttcgaacaga tccagggcaa ggagctctcc tacaacaacc ttctcgacct cgactccgcc
    10861 atgcgcctcg cccaagcatt ccacgagccg gccgccgcaa tcatcaaaca cacaaaccct
    10921 tgcggcgtgg caagacgcga ctcactcgtc gatgcattga aagccgccat cgaatccgac
    10981 cccgtctccg ccttcggcgg catcatcggc gccaaccgcg aattcgacgc tgatgccgca
    11041 gccgtcgtcg agtcgatgtt cctcgaagtg atcgtcgccc catccttcag cgacaaagcc
    11101 cgcgagatcc tggcgaaaaa gaaaaacctc cgcctgatcg tcgcccagcc cttgacgaat
    11161 gacaccgact accgcagcgc cgcaggaggg attctcgccc aaagcaggga ccggatcggc
    11221 tcgcgcgatg gatggaaagt cgtcactgaa cgccacccca cccccgaaga aatggacggc
    11281 ctcgaattcg cctggatcgt ctgcgcccac gtcaaatcaa acgccatcgt cctgacgaac
    11341 caatcgcaaa cagtaggcat cggcgccggc cagatgagcc gcgtcgacgc cgccaaagta
    11401 gcgatcatga agtcgatcct ccccatcacc ggaaccgtcg ccgccagcga cgccttcttc
    11461 cccttccgcg acggcctcga cctcctcgcc gacgccaaca tccgcgccat catccaaccc
    11521 ggcggctccg tccgcgacgc agaggtgatc gcagcggcga atgagcgggg tatcgcgatg
    11581 gtgtttacgg gggagcggca ttttcggcac tgaccccgcc cgatgcctga gtctacggtc
    11641 tcggacgcga ccgcaagaga cggtttgtcg acggtccgcc gagccctgcg acccctgaaa
    11701 ttccggatat tatcgtcgct cgcaaaatct tttcgcgaaa gggggtaggg aaaccagcgt
    11761 gtcagtcact cccctaccag agggagcttt gattgatggt gaaacgtgac tcggaccgag
    11821 gaatgttcgc ggagcccttc ggccaggcgg attgcgagtc tgagctggag actctgcgtg
    11881 agcggtacag cgaattttat ttcgagcaca gccctttcaa tgaggcggcc ctaaatgaga
    11941 aagtctactt aatcgttggc cgccgcgggt ctggcaagac ggcactatcg gagtactttt
    12001 cttttcaaaa gcgaatcccg aaccccgttt gcattgaaat acacgaaccg tccgtgtacc
    12061 aggaagtgct cgctgccctt gcaagtcgcg caccggaggc gcgtgtcgtc gcagtaccgg
    12121 cgctcacgaa agtctgggag tttgttatct ggtcgacgat ctttcactct ctaaaaacgg
    12181 agaacgttga cattcagcta gcttgtgaag tcccaacctc tcgcctcggc cgcgtgtccg
    12241 atgtcgtcaa gacgttcttt gagcacctca ccagcgtctt tcacgacggc caccgtggaa
    12301 accaggcgtc cggggacttc agcgacctct acgatgaagg ccaactcgag gccaacattg
    12361 cacgggttct tgagttcgcg caagagcgtc agattatcgt ggcaatggac acgctcgaga
    12421 aatatgacat tcgcgaagag tcactaatga cggcgatggc ggcgttagta caatacgcct
    12481 cgaagttcaa cctgcgatac gcctaccgtg gcgttcattt gaagctcttc ctctccggcg
    12541 aggtattccc ctacctcaag gaaaccgtcc tcctgaatcc actgaagaac atcaagcatc
    12601 cgctgtatct gctatggcga cctaaggagc tcttgcaact tatcacatgg cggttctggc
    12661 gctaccttac ctccagcagc gaggtatacc gggcgcgcct tagcgacgtc aactggagtc
    12721 gccataaaga ggtatttgag cgcatgtgga agccgttttt tggcagtgca cttgagaacc
    12781 gccgtggccg caaagagcgt acgtttgtat atgtgctaag acacacacag atgcgtccgc
    12841 gccaactcat ttttctatgc aatacgatag cgaatgacgc aattcttcgc ggtgacttcc
    12901 ccacattttc acccgagggc attcgggaag cagttaagaa ggccgaagcc gatctggcaa
    12961 acgaaattct taattcgtac gagttaacat acccttcggt agcacgtatc gttcaggccc
    13021 tgctcggcat gccgatgatt ttcgatgggt cggaattgga taagcgcgca aaggacactc
    13081 gcggcgagtg gccggacggc gaatattcgc ttgcgaactt tcgtcaattg gtcgctgagc
    13141 tcgggatcgt gggccgggtt aaggatcgca acgatgcagc gggttttctt gatgccgagt
    13201 tcgagtactc ggcgatggaa cgcttggcta tatctccacg cgaccagtgc gctatacatc
    13261 caatgttcta ttcaaaactg aacatcaaga tggatcaaga catacgggtg atgcctttct
    13321 ccgtggaacg cggagaactt gaagagcttc aggaaatctg actagggagt ctgcgtggac
    13381 caacagaggc gcgacggagc actgaccaag gatgagacaa taaatgcggt ggccggagtt
    13441 accgcggagc tcatgttcgc gctactacct ttgctcgtca ttacgatagt gatgagtttc
    13501 cggaacgaag ggtggcgcgc gcttacttca gctgagtggt cgtttgggtc ggcgatactt
    13561 tgcggacagg ctctcgtgaa gttcgttaca ggtctcgcta gcggttctaa accgagatgg
    13621 caacgagtgt cattcgctgt agcggccttg tttgtcttcg gtgtagtccc ggctttggtg
    13681 gtattggcac taatgatcgt ctcggagggt aacaccccac cgtggcttgt gcggatgcaa
    13741 attgtcgtgt ttgtggcaag catgatcgag ttcttcatct ttgggacgct aggccacctg
    13801 tggaaggagc gacgaagagg ttagctggca gctcgttgct gatgtggctg cgagatggcg
    13861 gcgcgcttcg cgcgaaatca gagcgtcacc aactccaagt gctcgatctc cctctccagg
    13921 aaatcgctgg cgattcttcg gaagcgttcc tcggcgtcgg tgaccaggaa ggtgtgtttg
    13981 ggggtgccgg acggtttgtt gcggaccgca ttctttacga atgacgccgt cgtctcggcg
    14041 ctgtcgacga tcgcgatttt gtcgccggta acctgttcga tcgtgcctct cagaatgggg
    14101 tagtgcgtgc agccgaggac caaggtgtcg atgttggcgt cgaggagggg ctcgaggtag
    14161 atctcggcga cttctctggc cacgtgcgtg ttggcccatc cctcctcggc taacgagacg
    14221 aagagcgggg cggcggattc gatcacttcg atcttcgggt cgatcaggcg gatcgctttc
    14281 gtgtatgcct gcgacctcac cgtcccctcg gtggcgatga cgccgacgcg gcctcgggtg
    14341 cgcgcgaccg cggcgcgcgc gccgggctcg acgacgccga tgacggggat cttcagcgac
    14401 gcctgcaaag ccggtagcgc ggcggcggtg gcggtgttgc aggcaacgac gagcatgccg
    14461 atgccgcggt cgatgagatg cgcggcggcc tggagcgagt agcgtgtcac cgtctcggcg
    14521 gatttcgttc cgtaggggac gcgcgcggaa tcgccgaggt agatgaggga gtggtgcggg
    14581 agggcgcggg cgatctcgcg gaagacggtg aggccgccga cgccggagtc gaagatgccg
    14641 atggaagtct tgttttggct gcttcccatt tcattcttcg tgcttgcaac ggcccctcat
    14701 ccggccttcg gccaccttct ccccatcaaa aagcgatggg gagaaggggc tcgattgggg
    14761 ggtggcgcga gatgcgtgag tagggatttt tctcaccctt cactcttcag atcgtcaggc
    14821 gcggtcatga cgccaccgcc ttctcgcttg atgttgtatt gctcgatttt cttgtagagg
    14881 ttcgatctgg gggtgtcgat ggcttcggcg gttttggaga cgttccagcc gttttcgcgg
    14941 agcttggcca ggatgaaggc tttttcggct tcgtctttga aatcctggag cttgtcgagg
    15001 cgcatggtcg tggtgatgat gtcgccggcg gcgcggaaga actcggcggg gagatcggtg
    15061 atgtcgatcg tgtcggcggg ggtcatgatc acgaggcgct cgatcatgtt gcgcaactcg
    15121 cggacgttgc cgcgccaggc ggcgtcctgc atcgccttcg ccgcggaggg cgtgaacttc
    15181 ttcgcgggac ggttgtactg gtccgagaac atcgtggcga aatgctgggc caggagcggg
    15241 atgtcgtctt tacgctcgcg cagcggcggc gtgcggacgg gaatgacgtt caatcgatag
    15301 aagagatctt cgcggaagcg gccgctcttg atctcgtcgg tcagatcctt gttcgtggcg
    15361 gcgatcacgc ggacgtcgac tttgatcacc gtcgccgatc cgacgggctc gacctcccct
    15421 tcctgtagca cgcgcagcac cttggcctgc gtgcgcagcg acatgtcgcc gatctcgtcg
    15481 aggaagatcg tgccgccgtc ggccgcgacg aacttgccgg tctgtttgcg gacggcaccg
    15541 gtgaacgaac ccttctcatg tccgaacagc tccgactcga tcaactcctc ggggatcgcc
    15601 gcgcagttga cctggatgaa cggcatcgcc tttctgtctg accggcgatg gatctcgcgc
    15661 gccacgagct ctttccccgt gcccgactcg ccctgaatca gcacggtcgc tttcgtcggc
    15721 gcggccagct cgatctgcgt cttgagattg accatcgatg ccgaatcgcc gacgagttga
    15781 tagtcgcggg cctcacgctc gcgcagctcc ccgacctctt cctcgagctt ctgctggcgg
    15841 acggcgttgc ggacggagag gaggagcttg tcgcgattga gcggcttctc caggaaatcg
    15901 aacgcgccgc gcttcgtgca ttcgacagcg gtggagatgt ccccatggcc ggacaccatg
    15961 atcactggcg acggcacttt ctgctctttc agattctcga ggacctcgat gccatcgaga
    16021 atcggcatct tgatgtcgag caggatggcg tcgtagggcg cggcagtgcg cgtggccttg
    16081 tcgatgccat cctgccccga ccgcgcctcg tcgatgcgat agccctcgta ctccagcgtc
    16141 atccggatcg cttcgcggat ggcagtctca tcgtcgatga ccagaattcg aaacggcatt
    16201 cagttacctc gctcgtgcat gagtccggcc aattgtatgg gcatgtactt ctgatctggc
    16261 cccatcgccc gcaaacccat acgcattgga tttgtcacgc ggagacgcgg ggaccgcgga
    16321 gaaggatggg ttacgtaggt cggactctcc agtccgactg cacgtgattg tcggactgga
    16381 gagtccgacc tacgtaaaac caaccctccg cgccctccgc gtctccgcgt gaaacgcttt
    16441 tcaagaagag cagatacaat ccggcgcgat atggacgtca ccatcacacg cgagcccaag
    16501 gttgcgaaag ctgaccacct ctttgtattg attgcagaaa acagcaagcc cgatgtcgcc
    16561 gtcgccgcgc aggcgatgaa agcgattgcg gatgcggggt tcaccgggaa ggcggatgag
    16621 tcgctcacga ccgttgccgg cgagccgaag aagatcacgc tgctcggcat cggcaagcag
    16681 gaagcgctca ctattcgcat tcttcgcacg gcgctctaca gcgtggcgaa gatggcgaag
    16741 aagcagcgcg accggaacat catcatcgtc ctcccctact ccgtggcgaa gctcgatgcg
    16801 gatgcgacga cgcgcattgc cgcctcggcg ctctcgggca gcgactacaa gtacgacacg
    16861 tacatcacga agaaagagga caagtcgctg ccgatcagcg cgcagttcgt gccacccgag
    16921 ggggtggatg cgaagcgggc gaaacagctc gacctcgaag cgaaggccgt cgctgcgggc
    16981 atccgcaccg ttcgcgacct cggcaacgcg cccggcaatc tcgtcaccgc caccttcatc
    17041 ggccaccgcg ccgaagaggt cgcgaaagag atcggcatca agtgcacggt ctacggcaag
    17101 cgcgagatcg agaagatgaa gatgggcggc ctgctggccg tcaacaaggg ctccgccgaa
    17161 gagcctcgct tcatcgtgct cgaatacgcg ccacgcaagg ctacgaagca cgtcgccctc
    17221 gtcggcaaag gcatcacctt cgactccggc ggcatctcca tcaagccgtc cgacaagatg
    17281 gaggagatga agttcgacat gtgcggtgcc gcggcggtca tcggcacgat ccaggcggcg
    17341 gccatgctgg cgctgccggt gcgcgtcacc ggcgtcatcg cgtcgacgga caatctcccc
    17401 agcggcagcg cctacaaacc gggcgacatc atcacgacga tgagcggcaa gaccatcgag
    17461 atcgtgaata cggacgccga gggacgggtg atcctgagcg acgcgctgca ctacgcgtcg
    17521 gaattgaagc ccgatcacat catcgattac gccacgctca cgggcgcgtg cgtggtcgcg
    17581 ctggccagcg aggcggccgg acttttctcg aacaacgacg aactggctca gaagttgatt
    17641 gagtgtgggg agcgcgtcgg tgagcgcctc tggagattac ccgagtggga cgactacaag
    17701 gaactgatcc gcagcgagtg ggcggacatg aagaactcgg gcggacggtg gggtggtgcg
    17761 acgacggcgg cagtatttct gaaggagttt gtcaattgtc catcgtgggc gcatctcgac
    17821 atcgccggca cggcatacgc ggagcacgaa acgtcgcgcg aggcgcgcgg agcgacgggc
    17881 gcgggcgtgc gcgtgacggt cgcgtttctc gaatcgcttg tgcgctcctg accgcgtgtg
    17941 ttcttgcagc atgtgacgcg cgtccgcccg caccggtgcg gatcgggccc gcgccgcgca
    18001 acgaagtcaa cttcgccacg gtcgactcgc cgagccttca gttcatcccg cggcagcagg
    18061 aagcgaagat ctggcagctc gacgaaaatc cgatcgtcgt tcccagcgat catctcgatg
    18121 cgtacctcgg atcgatcgac gcgccgcagt tcgcccgata cgaaacgctc gatctcacca
    18181 tcggcaagta caccacgctg gcaggcgatg gcttcgcgac cgtcgagatc ttccgttttc
    18241 ccgatttcgt caaagccttc ggcgcgtact cgatggcgaa gagcggcaac caccaattcc
    18301 tccagatccc caacgagtcc ttcgccaagg cgcactcgat tcacatctgg cgtgggccgt
    18361 actacgttcg cctcaccggc ggcggaccgg gcaacgcact cctgcaactg gcaacgttcg
    18421 tcgccgaccg gatgccaccc gcaccgaaca agccggcagt cttcgcgttc ctgccggaga
    18481 cggagcgtgt gccgggaagc gagcgttact ccgccgacgc cgcgttcggc caggcgttcc
    18541 tgggcaattc gtttcaggcc acgtacaacg tcggaaacga catgatcgaa ggcatcgtga
    18601 ttccggccag cagccgtcag gcagccgcaa acattctcga tcaatacaaa ggtctctacc
    18661 tccacaacgg caagctgctc gatccgatcc ccaacctcgg cgaagacaat ttcaccgccg
    18721 aggaccgctt cctcgggcgc gttgtagcgt tccgcatcga ccgcttcgtc gtcgccttca
    18781 acggttttcg cgagcgccag catctcgtcg atctggccac gaatgccgat cagcgcatcc
    18841 tcggcacgat ccgcaaacag ctcgtcacgg ccgacaagca agcggagaac gggaacgacg
    18901 gcgagaacac gtcaacgtta ccggcttggc tgcaacagaa gaaacggtag gaccatcacg
    18961 cgaagacgcg aaggcgcgaa ggggctaaac aaccttcttc gcgtcttcgc gtcttcgcgt
    19021 gagcgtcttc gcatgagccg ttttgcggcc tactccacta caaaatcgcg gcggacgtag
    19081 ccgagtacgc gcgtgcccat gattcggaag aggactttgg cggtgtactt tcctggcggc
    19141 agctcgaacg gctcgcgtag cgtgaggtag ccagttggaa cgcgggccgg gccggtgaga
    19201 ctgacacgtt tcgatttgaa tccgaccgtg gaaccgtgct cgtcgaggat gtagatgagc
    19261 agctcgatgc ctggcgatgc gttgggaagt tgcggcacga cctccgcgcg gttgaaggcc
    19321 acggcaagga cggacgtgcc gtcggccgag gtgactccgg catcgagcgt ggcaccggac
    19381 tgcggtacat cgttgagcag gatgtcggcg agttgcagag cgtccacgtt cttgcctggc
    19441 ggaacgtagc cgaatccctc gcgatacgac acctcggaac cgtgcggcag accattcacg
    19501 cgaacggtga tcttgccgtg cttccggccg gcgctgctga agccgaggac gtagacggtg
    19561 cgctcggccc gcatgatcgt gtcgacggca tcgccgaggt cgttgcgatt cacgatgacg
    19621 cgtccgccgg tgccgcgcga aagcatgtag agcgcgtcgt tgtcgagatc ggagagcgtg
    19681 tggcgaaggc cctggatgtc gatgctgtcc agcgtgacgc cggcggagac aaatgtttcg
    19741 tgcatctgtt tgacgacgtc gagcaggtgc gcatcgaggt tcggaggcct gccgttcgct
    19801 ttgatgtcgg tgatccgcgt cgaatcgaag ccctcggtga aaatgaggat gtgcttctgc
    19861 ccttccagag cgctgaggcg cgcggccacg tcgccgaggg cgtcgacttg atactcgatc
    19921 aggcgattac cctgcatctt ctgcatgtcc tggttggcct gaccgccggt gagtatttcg
    19981 ttgacctggt ctccgagtaa gggggagatt ccttccgcgg aaccgcccag ctccgtcgcc
    20041 gtcaaccatt gcgcgcgctc ggggctggag agcgcgacgc cgaggggatc ctgggctgag
    20101 cttgcgcgaa gcgtgtagat cgcgcgcgtg atcgccaccc ggtcagaaag aaaggaggag
    20161 acaaactgca caccgcggtt agacgaatac gtggcaacgc cgaagaagtc ggtggctgga
    20221 ttcgagcgcg caacggcctg ctccgcagcg gtctgcgcct tcttgattcg ggccggcgtg
    20281 gcgaagctca aatcgaacaa gaggaggtac agacggcgtt cgcggcgcgg ccgctcgacc
    20341 gcagtctgtg cagctggggt ggggactttc gcgaagtcga tggcgtcgaa atactcgatc
    20401 ggttgcggac gtccgtcgac aaacaactcg aacgcgtcgc gcgtcaggcc ctcgacgggc
    20461 tgattgttgc ccgaggtgat gtacacgggc acttccacga cttcgaccgt gacgttttcg
    20521 cggacttgcg cggcgaccga aagcgcgcaa acagcaatga gtgccaacgc gatccgccgg
    20581 ctcatgaccg ctcccacgtc ccgctcttgc cgcctgactt gcgcatgagc tgcagcgatt
    20641 caatcgtgat gcctttctcc gcgctcttgc acatgtcgta aatcgtaagg gcggcgacgg
    20701 cacacgcggt catcgcctcc atctccacgc cggtcttccc ttcgcaacga acgatggagt
    20761 ggacttcgat ggtgccggcg gccggatcga ggtcgaaggt gatgtcgatc gacgtgatgg
    20821 caagcggatg cgcgagcgga atgagattcg gcgtctgttt cgcggccaga acgccggcca
    20881 ctttggcagt cgtcagaaca tcgcctttgg gcagggcctg ggcgacgagc ttttccagcg
    20941 attccggcga catgcgcacc gtcgccacgg caacagcctc gcgcatggtg ttgcgcttcg
    21001 aggcgacatc gaccatcgag atgccgccgg tttcgtcgag atgagagaaa ttcttgctca
    21061 tgcgtctgtt ctccgctcct ccgcgcctcc gcgtgacaag ggttttttta ccctgagccg
    21121 aggagcgacg tccattctgc cagagtgttc acattcatca acggctcgcc cgcgaactgc
    21181 gcgcggagat cgtcttcgcc gagaatctct gcctcgccaa ccgccgcgac tccgcgaaga
    21241 tcgtaccgct tcgccgcgat ccgttcctgc agacgaggca agagcccggg atcgtagcct
    21301 gcgcagagca tctgcgtttt cccgctccag accggcgcga gcaggagcgc gttgctccgc
    21361 tcgaagcggt cacgcagaaa gcgcaggatg gccgtcgtga tcagcgggta gtcgacagcg
    21421 atcacgaaac agcgtccgct cgtatgtgcg agcgcgcagg cgacgccgaa caccggcgcc
    21481 tgatcgtcgt gaggactctc gtagatcgtc gtcagcggca cagccggtcc gccgtgccgc
    21541 tgcaccgcaa cgacgtcttc gaagacctcg gccgcggccg cagcgacacg gtcgaaaaag
    21601 agctccgtct tcgagcggcc catccgccgc gactcgccgc cgatgaggat gtaggcgttc
    21661 atcgccggta gctccgcgcc agttcggcga cgtcggcccc gcgccggaag cgcatgatcg
    21721 tccgccagtt gatcacggcc gtacgcagca caccctttcg cagaaaccgc cggcctgacg
    21781 tcgtcaccgt cagcggcagg agaatcgaac gtccgctccg tttggagcgg atggcgagat
    21841 cgtagtcctc catcagggga atctcgcgga agccgcccgt ctgcaggaaa cgctcgcggc
    21901 gaatgaactg cgcctgatct ccccagggac agcgcgtgat cgaagtccgc aagttgatga
    21961 gcgcggcggc cgcccggagt ttgcgcgccg gctccgcgaa ggcgatgcga aatccgccaa
    22021 agtccgcgcc gcttgcgagc gcgtcctcgg ctgcgcgtgc ggcgccgggc gggaggcgcg
    22081 tatcagcgtg caggaaaatc agcgcgtcgt tcaccgctgc gtttgcgccc agattcaact
    22141 ggcgcgcgcg cattggctcg cagagaagca atctggcacc ggcactcttg gcgaagcggg
    22201 cggtgcgatc gatgcttccg ccatccacaa cgatcacctc ggcggctcca gcaccaaacg
    22261 ctgactcgat cgtcgcaccg atccgctctt cttcgttcaa cgttgggatg atgacggaga
    22321 cagccattta cctggagcgc gccggcactg ctcgtcgcag catcacttct tcttgccttc
    22381 gcgctgcgcc accttcttcg cgtgccgcga ctggcccagc gtcacgttga tcgggttcga
    22441 gcggtcgaga aacggcacgg gaggtgcgga acggatgccc agctcgcgcc gcacctgcaa
    22501 cagcgccgcg tcctcgcgat gatccttcat cgcctgctgc accgtctcga gagccttctt
    22561 gcggttccct gccgccacgt atacgcgggc cagattcgcg tagtgattgc cgtcgtagaa
    22621 ctcgagctcg atggcgcgct tgcagagatc gatggcctgt ttcgttttct tctgcacgag
    22681 catcaggcag agtccaaagt acgagaggcc ggtcgcgctc ttcgcggttt tcgacaagat
    22741 caatggcgac gacgcgtcct ctgctccgta cgtgtcgaga aaaatcgtga gcgcgcgaag
    22801 atactcctcc tc
//
LOCUS       DATLVW010000425        18313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_17381, whole genome shotgun sequence.
ACCESSION   DATLVW010000425 DATLVW010000000
VERSION     DATLVW010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18313
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17381"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /gene="gndA"
                     /locus_tag="VNN25_12835"
     CDS             <1..1075
                     /gene="gndA"
                     /locus_tag="VNN25_12835"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HXH92460.1"
                     /translation="EKKLHFVGSGVSGGEEGARFGPSLMPGGSNESWEAIKPVFEAIA
                     AKSDSGPCVTHVGPDGAGHYVKMVHNGIEYGDMHLIAEAYEMLRKGLGANPEELAAIF
                     DEWNRGPLESFLIEITAHIFRVKDPETGAPLVDKVLDKAGQKGTGKWTAQIALDLAVP
                     IPTIAAAIDARVLSSIKEERVSASKVLRGPSDTTPVSDRKQFISDVHDALYVSKICSY
                     AQGMALIEAGSKEWNWSIDMREMARIWKAGCIIRAKFLDSIMLAYERKPDLRNLLLDD
                     EFSADAQKSQAAWRRTVAYAQANGIAVPSMSASLSYFDAYRSAELPQNLTQAQRDYFG
                     SHTYQRNDKGADTPFVHTDWIHK"
     gene            complement(1161..2417)
                     /gene="cls"
                     /locus_tag="VNN25_12840"
     CDS             complement(1161..2417)
                     /gene="cls"
                     /locus_tag="VNN25_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HXH92461.1"
                     /translation="MLFRKQKAHRYPRRPQLPIIRRRLPVRYHMKHQFDVRDVAFLQT
                     MHALTGAPMSQGNVVTILKNGVEIFPSMLAAIRAAKRTINLEFYIYWDGEIGRKFAEA
                     LAERARAGVEVKVILDAVGSAQMSQSLIDFLTRNGIDLEWYHPLRWYTLASFNHRTHR
                     KLLIVDGRIGFSGGVGIADNWLGDADSKEHWRDTVARVEGPVVSQMQFAFMDNWIKSR
                     GELLTGLDYFPEVPRGGSHLTQVLKSSPTEGSSMVKLLYIISIVSAAKSIYISNAYFV
                     PDKDTLRALEGAVRRGVDVRVIVPGEENDVPIARQASRWHYEHMLKRGIHIFEYQPTM
                     MHAKTMVVDGVWTTIGSSNFDDRSFRLNDEVNVNVYDDGIAAQMEAMFHEDLKQCEEV
                     LLRKWFKRGWPDKIKEKAAELFRPQL"
     gene            complement(2417..2920)
                     /locus_tag="VNN25_12845"
     CDS             complement(2417..2920)
                     /locus_tag="VNN25_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HXH92462.1"
                     /translation="MPDIFIPEVIEPDEKLPADLVALRHFARLMDEAVPIPGMKKRIG
                     LDAGLSLIPGVGEVIGAVLSTWIIVGALRHRVPLMRIGRMVLYILLDMSVGSIPVVGT
                     IFDWLFEENVINLNALLLHRDRTRPPRSFKAIAGAAVIILIVILSFSMLMLAGVLAAI
                     VWITSKR"
     gene            3085..3744
                     /locus_tag="VNN25_12850"
     CDS             3085..3744
                     /locus_tag="VNN25_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92463.1"
                     /translation="MNGFTIDFATKSKQLLSRFHHEIGKKFPRPGAQLAYVDLFFAGA
                     LRRSYALCRAFFDLILVENFTAAAPLIRLQLDTYLRTRAVSLVSDPQRFFEAIVTGTQ
                     LNHIKDSAGKRLTDTYLCERLGEGQPWVRELYRETSAFVHFSDKHALASLAGKDRNSF
                     TYGVCDAEEGVPDEAYDQAIVHFVRITNEVLQTFDICAEAAASVLTPSVATIAGPHPE
                     G"
     gene            complement(3766..6324)
                     /locus_tag="VNN25_12855"
     CDS             complement(3766..6324)
                     /locus_tag="VNN25_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXH92464.1"
                     /translation="MRRLTLCLFVLFFAVAVQAQNAIPTPDQFLGYPLGERFTPYARI
                     LDYFQLLTTRSNLITMQQFGETYEHRPLMLAVITSPKNRAALDAIRANVAALTDPSAT
                     TQARANEIAHTSPAIAWLAYGVHGNESSSSEASMQVASTLLSDPQSATLLDNLVILID
                     PLQNPDGRERYVQWFIRTRGADADPNADAFEHAEPWPGGRFNHYLVDMNRDWTWTSQR
                     ETLARVALYRTWNPQVFVDFHEMGYQSSYFFPPDARPVNANLPADVDTWLEKFGRANA
                     GAFTKKGWPFFVGEEFDLFYPGYGDSWPALHGAIGMTYEVGGGGRGGSAVLRDDGTVY
                     TLADRIARHYTTSMATLRTASENREGLLLYTYEMMRKQIASGQNTYLLVPNAPNFQPL
                     VEMLQRQKIALSQLNAAVTVKATRIDGDTAESHLFPAGTVLVSTRQPLGGLAQTLLEK
                     SPVFTKGYVEEQQKRTHSDEPDEFYDLTSWSLPLAMNVEAWVTASPVTGETHPYAASA
                     IPAFRAAPYGYLIDGIDPNIYRAAGRMLRDGVRFNVSDDVLTVNDRNFARGSLVILKG
                     NNKPDVDAALERLVHDTGVAVLPVDSGWTGATSFGSSKIHFVKDPRIALVGGQKVNAT
                     SYGMLWHTLDIDTPLPHTNLSAESLRNIDLSKFGVIVLPDGDYGDRFGKNAEKLKSWL
                     NGGGTLVVIKGASSFLREKEVEISKLKPWEAPKKKDDDKDKDKSPSTPEMYNDYRVPG
                     ATFRTAMNSHSYLTFGVPHSPFVLIEGSHAFLQVSHTVDNVVTIEKEKPLVAGVAWNE
                     SLDRLKGSIYLVSEPYGRGQVITFADEPHFRLFWRGTLPLFMNAVLYSPSFPRE"
     gene            complement(6715..8124)
                     /locus_tag="VNN25_12860"
     CDS             complement(6715..8124)
                     /locus_tag="VNN25_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92465.1"
                     /translation="MRILIVDDEEVLRDVLDAVLRREGFEVAMASSGEEALSVLDVDD
                     NIDLVILDIMLPGISGIDTLRAIRISNPSLPVIIITAFSSIDGAIEAMKHGAFHYVPK
                     PFKNEEVILTVNKALEQRRLSRENERLKAELSEKYAYANIIGKSEPMKKVYELIRLAA
                     PSRSNILVAGESGTGKELVAKAIHHASPRARNAFVTVNSGSLPPELLESSLFGHMKGS
                     FTGAIATKRGLFEVADGGSIFLDEIGNINLETQAKLLRVIQEKEFMRLGSVDTIKVDV
                     RIIAATNADLNKLMAENRFREDLYYRLNVITFPLPPLRRRREDIPLLVAHFLEKYAEE
                     NKRKVREVTPDAMRILLDHTWPGNVRELENTIERAVVLCTGDHIGPELLPDYLRFPVQ
                     TDQPAMIVPSDGISLKDAVSRYERGMILQSLEMANGVQKRAAELLQLKPSTLNEMMKR
                     LGIHTKSNAQVEEEVGVGD"
     gene            complement(8231..11056)
                     /locus_tag="VNN25_12865"
     CDS             complement(8231..11056)
                     /locus_tag="VNN25_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH92466.1"
                     /translation="MRRSFLTNLVILAVTVAIVAGAFASFQRKRSSFERIDFTFTRDK
                     GVVVVKGVDPGSGAETSGLRAGDQIWLIGDTPSNEIEGLQKTLRRIGQRVPMVVARGG
                     QTFRLTYLVPELKIDYPYLILSFIGFLYLAIGLFTLFRGGRRESTLFFFVTLLSFIVY
                     VYTPAGDIDFTYKVLQLVEELATILLPPLTLNFFLVFPRPIVRYRRLIAAMYIPPALL
                     AAWDFDLLVLGNRLAIAAPYRSLLLIQHWELVHFAIYFTLAVVALAYTYRTAAAVGKK
                     QIKWIYLGMALGFVPFLLVYLVPYLVIGSVKPIYSTISILPLALIPLAFAVSILKYKL
                     WDVEVVIKEILAYSVTFIFGMIAFSTINLILSHVIEERSALERNFLAFTSGLLIAGVL
                     IPVKGRIESVIEMLVYRDSYKHRRAIAEFAQELATFHDVHELISMMRERLRDALGLQR
                     MNLFTREGASLVIYDAEAGIPRRATIVDFGAMPSEGPLVLTDPRLPEGSDLPWQLLRT
                     GYRYLFPLRNRGELQGLLLLGTKRNEEPLSRDDLHLVESLCAPVALAIENSRLYSRLR
                     RQLEEIRALKEYNENIIESSSSAIAVVASDGTVLTANHAFWELIGGIGPAAIPLEATE
                     EPMSELFPPYDEMRRTKARSITTNFENRNGEEKEVTITASPLNADEPDGARVLVIGDI
                     TDRVRLERELQDKERLASLGLLAAGVAHEVNTPLTGISSYAQLLLADTKPDDPRYRLL
                     KKMEAQSFRASHLVNNLLDLIANRPRSREIVNIRELITATVALHEDLFKPKNIHVHVA
                     PMDGTRVQGNFHDLQQVLTNILLNARDAVSDGGNIWITTEECATNLSIRIKDDGKGIP
                     ADIISRIFEPLVTTRRGQGGTGLGLAITKRILDAANGVVTVESTPGNGATFTITLPLN
                     GSGSADPSTSGSESEDAVVAGSGPRVH"
     gene            11317..13257
                     /gene="asnB"
                     /locus_tag="VNN25_12870"
     CDS             11317..13257
                     /gene="asnB"
                     /locus_tag="VNN25_12870"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH92467.1"
                     /translation="MCGLTGFVASSASSADELTGLLRPMCDAIAHRGPDDSGEWIDPE
                     LGVALGFRRLAIIDVSPAGHQPMVSASGRYVVTFNGEIYNFEALRTELREAGLAPVFR
                     GHSDTEVMLAAFEEWGVDASVPRFNGMFAIALWDRKLRRLYLMRDRMGVKPLYYGVAG
                     RTFLYGSELKALRRHPDFDGTIDRGALGLYFRFLYVPAPYSIYEGIRKLMPGTILTFD
                     PATREMETTTYWSAADAAARGMEHRFQGSEEDASRAAEELLRDAVRIRMIADVPLGTF
                     LSGGIDSSLVTALMQAESSTPVRTFTIGFTETDYDEAPYAAAIARHLGTNHTELYMTP
                     DDVVNVIPKLTAMYDEPFADSSQIPTHLVAMLARRHVTVSLSGDGGDELFGGYNRYFI
                     GQRLFQRLAALPAWTRPAIGRALASLSPRGWDRLLSLGRPLLPSSLRQANPGHRIHKV
                     ARVIAANDLDAMYFELVSHWSNLVLQAGDAEAPVTAQAARLPIKDPVERMMYFDQISY
                     LPDDILTKVDRASMAVSLESREPLLDYRLVEFAWTLPLSMKVQNGVGKRVLRRVLDRY
                     VPKELIDRPKMGFGIPLESLLRGRLRDWAESLLDPSTMRAQGFLDTAMVQARWKDHLD
                     GKDQWKFYLWGVLMFQQWVRDN"
     gene            14017..14988
                     /locus_tag="VNN25_12875"
     CDS             14017..14988
                     /locus_tag="VNN25_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92468.1"
                     /translation="MRNEDSAQPGSLRGEPGFRIPSSLKAGTLMNRSRVFSVIFSPLV
                     ALLGACTSAITSTPTITICKQTQPGGGAGFQFTTTNGGLGSPFGPFTLNDTQCHTMLV
                     NAGQDHYNAFTENVPAGWLLTNISCNATTSLVTIVGANGNPGFQPGDNTVTIDLNEPH
                     VTCTFVNAQTPHCCGFNLDLSTGQGTVIDPLWTVNGGNAYVTPPSTAWTVNMPPAQWI
                     QPVASPTPSGNVPPGVYKYVVSFTPTPCAMGHLELSGTFAADNDAGAMLDGVHIGTAC
                     TNCFNTAAVPFAVNPLTLTSHVLEINVNNLGGFSGLIVNAHVKRICP"
     gene            complement(15026..15904)
                     /locus_tag="VNN25_12880"
     CDS             complement(15026..15904)
                     /locus_tag="VNN25_12880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXH92469.1"
                     /translation="MKTVSIIIPTYNYGRFLRDAIDSALAQTYRPLEIIVVDDGSTDD
                     TPQILAEYGERIRVIHQDNGGVGAARNTGIAAAKGDYLSFLDSDDIMKPQLLEHEIAR
                     FIADPDLGLVHCAVEQVDRDGKLLFVSQNGMEGWVATELLRLDREVITAPGSSIMVPK
                     RVAQEIGGYDKTLQPSEDWDFCYRLAVRYRIGYVREPLLKYRLHGGGIHLNVAQMEIS
                     MLRALDRAFQNPAVRSLRNHSYGRIHRVLAGCYFQLHQPRRFFRHMIKSLRYDLRNLG
                     YFAAYPMRVVARAFAR"
     gene            complement(15950..17569)
                     /locus_tag="VNN25_12885"
     CDS             complement(15950..17569)
                     /locus_tag="VNN25_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HXH92470.1"
                     /translation="MGDPQPFEQLAFGDGDLLADTGPGAPYPTPRNGPGLTSRVVRGS
                     FWNFGGQGVTMLATLVATPFTIRLLGAQSYGVLTLVHVLIGYLGFGDLGMGTASTRFG
                     SHAYAAGDDRGEATAIWTALALAVVPATTLAILLVLGAPLLVEHGLHLPLSLRPVAVV
                     AIRLAAVGFFARAVAGVFNTPAFVRLRMDLVALISTGTAAGQILLIPVVLLLGGGLTS
                     AVMVVAGAAIATALLYAIAGLKLLPSLRHPHVDRALLKPLARFGGALVVSSMTATLLA
                     SSEKVLLPRFASLQALAFYSVAFTLAYLPTQLPSTIVQSLVPAFSQLQMRPGREEMQL
                     LYRRALHGMLFWAVPAAMFVCAVAQPFFTIWAGAEFGRESTLPLYLLMGGIVAEIMTY
                     VPYALLIALGRTDIVARLNLSLVIPYVILSAVLIQQFGAVGAAIAWSLRTLTGAVAVA
                     YFAQRTSGFAYAPWPDNKRAYLAAAAVLLLPVPIVTILTTSAMVRAVVAAAAVAAYAL
                     LILTRVLTDDERKAFQRLLPIPRQRRDSTDV"
     gene            complement(17562..>18313)
                     /locus_tag="VNN25_12890"
     CDS             complement(17562..>18313)
                     /locus_tag="VNN25_12890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXH92471.1"
                     /translation="GTPFDHSPIERDEQTVMRRFVRPGDTVFDVGANYGVHTALLAEL
                     AGSRGHVFAFEPNPELITGLARIVERLGNATLSRYALSDEDTKAVFFVPPDRTMSSLA
                     DWTSLERMPSQRQQAALGKTSTITVQQQRIDDLVSKGELPVPNFIKCDVEGAELKVFR
                     GGKETLDRVEAPVVLFEAGPESAHGFGFGMTDAADFLVGLQRPGYQLFEVQAGGMLRP
                     VSSAELKKQNQNVLAVPHNEHARCPELASHG"
BASE COUNT         3536 a         5393 c         5860 g         3524 t
ORIGIN      
        1 agagaaaaag ctgcacttcg tcggctcggg cgtgtcaggc ggtgaagaag gcgcgcgctt
       61 cgggccgtcg ctgatgcccg gcggctcgaa cgaatcgtgg gaagcgatca agccggtctt
      121 cgaggcgatc gcggcgaaga gcgattcggg gccgtgcgtg acgcacgtcg gtcccgacgg
      181 cgccggccat tacgtgaaga tggtacacaa cggcatcgag tacggcgaca tgcacttgat
      241 cgccgaggcg tacgagatgc tgcgcaaagg actgggtgcg aaccccgagg agctggcggc
      301 gatcttcgac gaatggaacc gcggcccgct cgagtcgttc ctcatcgaga tcaccgcgca
      361 catcttccgc gtcaaggatc cggagaccgg cgcgccgctc gtcgacaagg tgctcgacaa
      421 ggcaggacag aaaggcaccg gcaagtggac ggcgcagatc gcgctcgacc tggccgtgcc
      481 gattccgacg atcgctgcgg cgatcgatgc gcgcgtgttg tcgtcgataa aggaagagcg
      541 cgtcagcgct tcgaaggtgc tgcgcggacc gtccgatacg acgcccgtca gcgaccgcaa
      601 gcaattcatc agcgacgttc acgacgcgct gtacgtttcg aagatctgtt cgtacgcgca
      661 gggcatggcg ttgatcgagg cggggtcgaa ggaatggaac tggtcgatcg acatgcggga
      721 gatggcgcgc atctggaagg ccggctgcat catccgcgcg aaattcctcg actcgatcat
      781 gctcgcctac gagcgcaaac cggatctgcg caacctgctc ctcgacgacg aattttccgc
      841 cgacgcgcag aagtcgcagg cagcatggcg ccgcaccgtt gcctacgcgc aggccaacgg
      901 aatcgccgtg ccgtcgatgt ccgcgtccct cagctacttc gatgcgtatc gcagcgccga
      961 gctgccgcag aatctcacgc aagcgcaacg cgattacttc ggctcacaca cgtaccagcg
     1021 caatgacaaa ggtgcggaca cgccgttcgt gcacactgat tggatccaca aataggcggc
     1081 cgaaaagcat caccacagag acacagagat cacagagaaa ccctctgtgt cctctgtgtc
     1141 tctgtggtta agaaggtttt tcacaattga ggccggaaga gctcagcagc tttctctttg
     1201 atcttgtccg gccagccgcg cttgaaccac ttgcgcagaa ggacttcttc gcactgcttc
     1261 agatcctcgt ggaacatcgc ctccatctgc gcggcgatgc cgtcgtcgta gacgttgacg
     1321 ttcacctcgt cgttgaggcg gaagctgcgg tcgtcgaagt ttgatgatcc gatcgtcgtc
     1381 cagacgccgt cgaccaccat cgtcttggca tgcatcatcg tcggctggta ttcgaaaatg
     1441 tggatgccgc gcttgagcat gtgctcgtag tgccagcggc tggcctggcg cgcgatcggg
     1501 acgtcgttct cttcgccggg cacgatgacg cgtacgtcga cgccgcggcg aacggcgcct
     1561 tccagcgccc gcagcgtgtc tttgtcgggc acgaagtagg cgttgctgat gtagatcgac
     1621 ttcgcggcgc tcacaatcga gatgatgtag agcagcttca ccatcgagct tccctcggtc
     1681 ggcgaggact tgagcacttg cgtcaggtgt gagccgcccc gcggaacttc ggggaagtag
     1741 tcgagaccgg tgagcagctc gccacggctc ttgatccagt tgtccatgaa cgcgaactgc
     1801 atctgcgaga cgaccgggcc ttcgacgcgg gccaccgtgt cgcgccagtg ctctttcgaa
     1861 tcggcatcgc cgagccagtt gtccgcgatg ccgacgcccc cggagaagcc gatgcgcccg
     1921 tcgacgatga gcagcttgcg atgggtccgg tgattgaagc tggcgagggt gtaccaacgc
     1981 agcgggtgat accactccag gtcgatgccg ttgcgcgtca ggaagtcgat cagcgattgc
     2041 gacatctgcg cggagccgac agcgtcgaga atgaccttga cttccactcc tgcgcgcgcc
     2101 cgttcggcca gcgcctcggc gaacttgcgg ccgatctcgc catcccaata gatgtagaac
     2161 tcgagattga tcgtgcgctt cgcggcccgg atcgcggcca gcatcgaagg gaagatctcc
     2221 actccgttct tcagaatcgt gacgacgttt ccctgcgaca tcggcgcgcc ggtcaaggcg
     2281 tgcatcgtct gcaggaacgc gacgtcgcgc acgtcgaact gatgcttcat gtgatagcgc
     2341 accggcaggc gccgccggat gatcggcaac tgcgggcgcc gcggatagcg atgagccttt
     2401 tgcttgcgga agagcatcag cgcttcgatg tgatccagac gatggccgcg aggacgcctg
     2461 cgagcatgag catggagaag ctgaggatca cgatcagaat aatcacagca gcaccggcga
     2521 tcgccttgaa cgatcgcggc ggacgcgtgc ggtcgcgatg aaggagcagc gcgttgaggt
     2581 tgatgacgtt ctcttcgaac agccagtcga agatggtgcc aacaaccgga atcgatccaa
     2641 cggacatgtc cagaaggata tagaggacca tccggccgat gcgcatcaac ggcacgcgat
     2701 gccgcagcgc gcccacgatg atccaggtgg acagcaccgc gccgatgact tccccgacgc
     2761 cgggaatgag gctcagtccc gcatcaagcc cgatgcgctt cttcattccc ggaatcggaa
     2821 ccgcctcgtc catgagccgc gcgaagtgac gaagtgcaac cagatccgcc ggaagcttct
     2881 cgtccggctc gatcacttcg gggatgaaga tgtcaggcat gctcaaggct caatgcaacc
     2941 tatggcccat gcagcacaga caagagtgtc tgtgccacat ggcttgtcgt tttgcgagat
     3001 gaattctata gaagcaggca gagcggccta tgttcggcct cagggagacc cgagtcggca
     3061 agtccaagcg cgtatcatct ttcagtgaac ggattcacaa tcgattttgc tacgaagagt
     3121 aagcagctac tctcgcgctt tcatcatgag atcggcaaga agttccctcg accgggtgct
     3181 cagttggcct acgtggatct cttctttgcg ggagcactac gacgatccta cgcactgtgc
     3241 cgcgcgttct ttgacttgat tcttgtcgag aacttcacgg cggcggcgcc gctcattcgg
     3301 ctgcaactcg acacgtatct gcgcacccgt gctgtatctt tagtttcaga tccccagcga
     3361 tttttcgaag ccatcgttac gggcacccaa ctcaaccaca tcaaggactc cgccggaaag
     3421 cggctcacgg acacgtatct gtgcgaacgc ctcggagaag gacaaccatg ggtccgcgaa
     3481 ctttaccgtg agacgtccgc tttcgttcac ttttcggaca aacacgccct cgcatcgcta
     3541 gcaggcaagg atcgcaactc cttcacctat ggagtctgtg acgccgaaga gggtgttcct
     3601 gatgaggcgt acgaccaagc aattgtccac ttcgtccgga ttacgaacga agttcttcag
     3661 accttcgaca tttgcgccga ggcggcagca tcggtcttaa ctccgtcagt ggcgacgatt
     3721 gccggaccgc acccggaagg ttaagcgacc accgattgaa ccacctcact ccctcggaaa
     3781 actcggcgag tacagcaccg cgttcatgaa caggggcagc gtgccgcgcc agaagagccg
     3841 gaagtgcggc tcgtcggcga aggtgatgac ttggcccctt ccgtacggtt cgctcacgag
     3901 atagatcgat cctttcaagc ggtcgagcga ttcgttccag gccacgccgg cgacgagcgg
     3961 tttctccttt tcgatcgtca ccacgttgtc gaccgtgtgg ctgacttgca gaaacgcgtg
     4021 cgagccttcg atcaacacga acgggctgtg agggacgccg aaggtcagat acgaatggct
     4081 gttcatcgcc gtgcggaacg tcgcgccggg tacgcggtaa tcgttgtaca tctccggcgt
     4141 tgacggcgac ttgtctttgt ccttgtcgtc gtcctttttc ttcggtgcct cccacggctt
     4201 gagcttggag atctcgacct ctttctcgcg caggaagctg ctggcacctt tgatgacgac
     4261 gagcgtgccg ccgccgttga gccagctctt cagcttctcc gcgttcttgc cgaagcggtc
     4321 gccgtagtcg ccgtcgggaa ggacgatcac accgaacttg gagagatcga tattgcgcag
     4381 cgactcggcg gatagattcg tgtgcggcaa cggcgtgtcg atgtcgagcg tgtgccacaa
     4441 catgccgtac gacgtggcgt tgaccttctg cccgccgacg agggcgatgc gaggatcttt
     4501 gacgaagtga atcttcgacg agccgaaact cgtggcgccg gtccagccgg aatcgaccgg
     4561 cagcacggcg acgccggtat cgtgcacgag gcgctccagc gcggcatcga catcgggctt
     4621 gttgttgcct ttgagaatga ccagcgagcc gcgcgcgaag ttacggtcgt tcacggtgag
     4681 cacgtcatcg ctgacgttga aacggacgcc gtcgcgcagc atgcggccgg cagcgcggta
     4741 gatattaggg tcgatgccgt cgatgaggta gccatacggc gcagcgcgga aggcggggat
     4801 ggcgctcgcg gcgtacggat gcgtctcgcc ggtcacgggc gatgcggtca cccatgcctc
     4861 gacattcatc gccagcggga gcgaccacga cgtcagatca tagaattcgt cgggctcgtc
     4921 agaatgcgtc cgcttctgct gctcctcgac gtagcctttg gtgaagacgg gcgacttctc
     4981 gagcagcgtc tgggcgagtc cgccgagcgg ctggcgcgtg ctgacgagga cggtgccggc
     5041 ggggaagaga tgcgattcgg cggtgtcgcc gtcgatccgc gttgccttta ccgtcaccgc
     5101 tgcattgagt tgcgacaacg cgatcttctg gcgctgcagc atctcgacga gcggctggaa
     5161 attcggcgcg ttgggaacga gcagatacgt gttctgcccc gaggcgatct gcttgcgcat
     5221 catctcgtag gtgtaaagca gcagaccttc gcggttctcc gatgccgtgc gcagcgttgc
     5281 catcgacgtc gtgtaatgtc gcgcgatgcg gtcggcgagc gtatagacag tgccgtcgtc
     5341 gcgcaggacg gccgagccac cgcggccgcc gccaccgacc tcgtaggtca tcccgatcgc
     5401 gccatgcagc gccggccacg aatcgccgta gcctgggtag aagaggtcga actcctcgcc
     5461 gacgaagaac ggccatccct ttttcgtaaa ggcgccggcg ttggcgcggc cgaacttctc
     5521 gagccacgtg tcgacgtccg cgggcaagtt ggcgttgacc ggccgcgcgt caggtgggaa
     5581 aaagtacgac gactggtaac ccatctcgtg aaagtcgacg aagacctgcg ggttccacgt
     5641 acggtaaagc gccacgcgcg cgagcgtctc gcgctgcgac gtccaggtcc agtcgcgatt
     5701 catgtcgacg aggtagtgat tgaaccggcc acccggccac ggctctgcgt gctcgaaggc
     5761 atcggcgttc ggatcggcgt ccgcgccgcg cgtgcgaatg aaccactgca cgtaacgctc
     5821 gcgcccgtcg ggattctgca gcggatcgat gaggatgacg aggttgtcga gcagagtcgc
     5881 tgactgcggg tcgctcagca gcgtactggc cacctgcatc gacgcctcgg aggacgacga
     5941 ttcgttccca tgcacgccgt acgcgagcca tgcgatagcc ggcgaggtgt gggcgatctc
     6001 gttggcccgc gcctgcgtcg tggcgctcgg gtcagtgagt gcggctacgt tggcgcggat
     6061 ggcgtcgagc gccgcgcggt tcttcggcga cgtgatcacc gccagcatca gcggtcgatg
     6121 ctcgtacgtc tcaccgaact gctgcatcgt gatcagattc gatcgcgtcg tcagtagttg
     6181 gaagtagtcg agaatgcgcg cgtacggcgt gaagcgctcg ccgagcggat aacctaggaa
     6241 ctggtcaggc gtcgggatgg cattctgagc ctgaacggcc acggcgaaga acagaacgaa
     6301 aaggcagaga gtgagtcgtc tcataagaag agtgaagagt gaagcgtgaa gggtgaaaaa
     6361 ggaagaggaa atgtgaagtt tgaagggtgc gcaggtgtcg acgtggccga tctgcagaag
     6421 tcatccaagg tgtcatcctg agacagccgg agccgccatt cgtactcttt cccctcgcgg
     6481 cgggcgatgt ggcggcgtag gacggcgaag gatctccgaa tgcgaggcat gtggcatgtg
     6541 cgtcgtaatt tgagatcctt cgccgtcctc cggcgccgag cgtatttagc gcgagaaacc
     6601 tcgagtagcg ccggcgcctt cggcaggctc aggatgacac taatctcgtt gtcgttcggt
     6661 actcttgctc gaggccttac acgtagtgcg gaggacccag gatgagagcg gcgttcaatc
     6721 gccgacgcct acctcttcct ccacctgcgc attcgacttt gtgtggatgc cgaggcgttt
     6781 catcatctcg ttcagggtgg agggcttgag ctgcagcagt tccgcggcgc gtttctggac
     6841 gccgttggcc atttccagcg actgcaggat catgccgcgc tcgtagcggg agacggcgtc
     6901 tttcagcgag attccgtcgg aggggacgat catcgctggc tggtcggtct ggacggggaa
     6961 gcggagatag tcggggagta gctcggggcc gatgtggtcg ccggtgcaga ggacgacggc
     7021 gcgctcgatc gtgttctcca gctcgcgcac gttaccgggc caggtgtggt cgaggaggat
     7081 gcgcatcgcg tccggcgtca cttcgcgcac tttgcgcttg ttctcttccg cgtacttctc
     7141 gaggaagtga gcgacgagga gggggatgtc ctcgcggcgt ctgcgcaatg gcggcagcgg
     7201 gaaggtgatg acgttgagcc ggtagtagag atcttcgcgg aagcggttct cggccatcag
     7261 cttgttcagg tctgcattcg ttgcggcgat gatgcgtacg tcgactttga tcgtgtcgac
     7321 ggatccgagg cgcatgaatt ctttttcctg gatcacgcgg aggagcttgg cctgcgtctc
     7381 cagattgatg ttgccgatct cgtcgaggaa gatcgagccg ccgtcggcga cttcgaacag
     7441 gccgcgtttg gtggcgatgg cgcccgtgaa cgagcccttc atgtggccga agagcgacga
     7501 ctccagaagc tcgggaggca atgagccgga gttgacggtg acgaaggcgt tgcgcgcgcg
     7561 cgggctggcg tggtggatcg ctttggcgac gagctctttg cccgtgccgg actcgccggc
     7621 gacgagaatg ttcgaccgcg acggcgcagc cagccggatc agctcgtaca ccttcttcat
     7681 cggctcggac ttgccgatga tgttggcgta ggcgtacttc tcggacagct ccgctttgag
     7741 cctctcgttt tcccgtgaga ggcgccgctg ctccagcgct ttgttgacgg tgaggatgac
     7801 ttcttcgttc ttgaacggct tcggaacgta gtgaaacgcg ccgtgcttca tcgcttcgat
     7861 ggcgccgtcg atcgaggaga aggcggtgat gatgatgacg ggaaggctgg ggttggagat
     7921 gcggatggcg cgcagggtgt cgatgccgct gatcccgggc agcatgatgt cgaggatgac
     7981 gaggtcgatg ttgtcgtcga catcgagcac cgaaagcgcc tcttcgccgc tcgaagccat
     8041 ggccacttcg aacccttcgc ggcgcaaaac ggcgtcgagc acgtctcgca ggacttcttc
     8101 gtcgtcgacg atgaggattc gcatgtgtga gtcagggatc agagatcagt ggatcagaga
     8161 tcagcggttc tccggttatt gattcgttcg tatgtttgtg ttcggcactt ggcaacgcag
     8221 caaccgtcaa tcaatgaacc cgcggaccag agccggcaac caccgcatct tccgattctg
     8281 atccactggt tgacggatcg gcggatccgc tgccattgag tggcagtgta atcgtgaatg
     8341 tggcgccgtt accgggggtg gactcgactg tcacgactcc gtttgcggcg tcaaggatgc
     8401 gtttggtgat cgcgagaccg agtccggtcc cgccctggcc gcggcgcgtc gtcacgagcg
     8461 gctcaaagat ccggctgatg atgtcagccg gaatgccttt gccgtcgtcc ttgatgcgga
     8521 tcgagaggtt cgtcgcgcac tcctcggtgg tgatccagat gttgccgccg tccgaaacgg
     8581 catcgcgcgc attgaggagg atgttggtaa gaacctgctg cagatcgtgg aagttgccct
     8641 gaactcgggt gccgtccatc ggtgccacgt gcacgtggat gttcttcggc ttgaagaggt
     8701 cttcgtgcaa ggcgacggtt gcggtaatga gctcgcgaat gttgacgatc tcgcgggacc
     8761 gcgggcggtt ggcgatgaga tcgagcagat tgttgacgag gtgcgaggcg cggaaggact
     8821 gcgcttccat cttcttgagc agccggtagc gcggatcatc gggcttcgta tccgccagaa
     8881 gcagttgtgc gtacgacgag atgccggtca gcggtgtgtt gacctcatga gcgacgccgg
     8941 cggcgaggag ccccagcgac gccagccgct ccttgtcctg cagctcgcgc tcgagacgca
     9001 cgcggtcggt gatgtcgccg atgaccagga cgcgcgcgcc gtccggctcg tcggcattca
     9061 gcgggctggc cgtgatcgtg acttccttct cctcgccgtt ccggttctcg aagttggtcg
     9121 tgatcgagcg cgctttggtg cggcgcatct cgtcgtatgg cgggaagagc tcgctcatcg
     9181 gctcctcagt cgcttcgagc gggatcgcgg ctggcccgat gccgccgatg agctcccaga
     9241 acgcatgatt cgcggttagg accgtgccgt ccgaggcgac gaccgcgatg gccgacgacg
     9301 acgattcgat gatgttctcg ttgtactcct tgagtgctcg gatctcttcg agttgccggc
     9361 gcaggcggct gtagagccgg gagttctcga tcgccagcgc caccggcgcg catagcgact
     9421 cgacgaggtg aaggtcatcg cgcgacaacg gctcctcgtt gcgcttggtg ccgaggagga
     9481 ggaggccctg aagctcgccg cggttccgca acgggaagag gtagcgatat ccggtccgca
     9541 gcaactgcca ggggaggtca cttccttcgg gtagccgcgg atcagtgagt accagcggtc
     9601 cctcggacgg catggcaccg aagtcgacga tcgtagcccg ccgcggaatg cccgcctcgg
     9661 cgtcgtagat gacgagcgat gcgccctcgc gcgtgaagag gttcatgcgc tgaaggccga
     9721 gcgcgtcgcg caaccgctcg cgcatcatcg agatcagctc atgcacgtcg tgaaacgtgg
     9781 ccagctcctg cgcgaattcg gcgatcgccc ggcggtgctt gtagctgtcg cggtagacca
     9841 gcatctcgat gaccgactcg atgcggcctt tgaccggaat cagcacaccg gcgatcagga
     9901 gtcccgaggt aaaggcgagg aagttgcgct cgagtgccga tcgctcttcg atgacgtgcg
     9961 agaggatcag gttgatcgtc gaaaaggcga tcatgccgaa gatgaacgtc accgagtacg
    10021 ccaggatctc cttgatgacg acttcgacat cccacagttt gtacttgagg atcgagaccg
    10081 cgaacgcgag cggaatcagc gccagcggca ggatggagat cgtcgagtag atcggcttca
    10141 ccgaaccgat caccaggtac ggcacgaggt agacgagcag gaacggcacg aagccgagcg
    10201 ccatgccgag gtagatccac ttgatctgtt tcttgccgac cgcggctgct gttcggtagg
    10261 tgtacgccaa tgccaccact gccaacgtga agtagatggc gaagtggacg agctcccaat
    10321 gctgaatcag aaggagcgag cggtacggtg cggcgatggc cagacggttg ccgagaacga
    10381 ggaggtcgaa gtcccaggcc gcgagcagcg caggcgggat gtacatcgcc gcgatcaggc
    10441 gccgatagcg aacgatcggg cgcgggaaga ccaggaagaa gttcagcgtc agcggcggaa
    10501 gaaggatcgt ggccagctct tccacgagct gcaggacttt gtaggtgaag tcgatatcgc
    10561 ccgccggcgt gtagacgtag acgatgaacg acagcaacgt gacgaagaag aagagggtcg
    10621 actcgcgacg gccaccgcgg aagagtgtga agagaccgat agcgagatag aggaaaccga
    10681 tgaagctgag gatcaggtag gggtaatcga tcttcagctc gggaacgagg taggtgagac
    10741 gaaacgtctg cccccctcgg gcgacgacca tcggcactct ctggccgata cggcgaagcg
    10801 tcttctggag tccttcgatc tcgttggaag gcgtgtcgcc gatgagccag atctggtcgc
    10861 cagcccgcag cccgctggtt tcggcgccgc tgccggggtc gacgcccttg acgacgacga
    10921 cgcccttgtc gcgggtgaag gtgaagtcga tccgctcaaa ggaggagcgt ttgcgctgga
    10981 atgatgcgaa cgcaccggca acgatcgcca ccgtcacggc gaggatcacc aggttcgtga
    11041 ggaaagagcg gcgcatgaga gtgagaaccg acgcggtgca agtgcattca acgtgagtgc
    11101 cgccgcagaa ttcaatctaa atcattgaac gcaaacgtcc ggcgcgttga attggcgctg
    11161 gcatcatgcc tccattctcc aagggatcga atttggtgag cagatatttg gaggatgtaa
    11221 gccctccagc cgtgaaaccg tttgcgtaat tcggagatcg gttgccatct ggcccgtggc
    11281 cgcttcgaaa cccggagtcc gcgctagact ccgccgatgt gcggcctgac cggtttcgtt
    11341 gccagctccg cgagcagcgc tgatgagctg accggcctcc tgaggccgat gtgcgacgcg
    11401 attgcccatc gtgggccgga tgactccggc gagtggatcg atcccgagct cggggtcgcc
    11461 ctcggattcc ggcggctggc catcattgat gtttcgccgg cggggcatca gccgatggta
    11521 tcggcctcgg gccggtacgt cgtgacgttc aacggcgaga tatacaactt tgaggcgctt
    11581 cggaccgagc ttcgtgaggc cggcctggcg ccggtgttcc gtgggcattc ggataccgag
    11641 gtgatgctgg ctgcgttcga ggagtggggt gtggacgcgt cggtgccgcg attcaacggc
    11701 atgtttgcca tcgccctgtg ggatcgcaag ctgcgccggc tctacctgat gcgcgaccgg
    11761 atgggcgtga agccgctcta ttacggtgtt gcggggcgga cgttcctcta cggatcggag
    11821 ctgaaagcgc tgcggcggca tccggacttt gacgggacga tcgaccgcgg ggcgctcgga
    11881 ctctactttc gcttcctcta cgtcccggcc ccctacagca tctacgaagg catccgaaag
    11941 ctgatgccgg gcacgatcct cacgttcgat ccggccacga gggagatgga gacaacgacc
    12001 tactggtcgg cggcagacgc ggcggcccgc ggaatggagc atcgttttca gggatcggaa
    12061 gaagacgcat cgcgtgcagc cgaagagttg cttcgcgacg cggtgcggat acggatgatt
    12121 gcggacgtcc cgctaggcac gttcctctcc ggcggcatcg attcctcgct cgttacggcc
    12181 ctgatgcagg cggagagctc cacgcccgtt cggacgttca cgatcggatt cacggagacc
    12241 gactacgacg aggctcccta cgctgcggcc atcgcccgcc acctcggcac gaaccacacc
    12301 gagctttaca tgacgccgga cgacgtcgtg aacgtcattc cgaaactgac ggccatgtac
    12361 gacgagccgt ttgccgattc ctcgcagatc ccgacccatc tcgtggcgat gctggccagg
    12421 aggcacgtta cggtgagcct ctccggcgac ggcggcgacg aattgttcgg aggctacaac
    12481 cgctacttca tcggccagcg gctgtttcag cgtttggcag ccttaccggc ttggacgcgt
    12541 cccgcgatcg gccgggcgct cgcttcgttg tctccgcgcg ggtgggatcg ccttctctcc
    12601 ctcggacggc cgttgttgcc atcgtcgctt cgtcaggcga atcccgggca tcgtattcac
    12661 aaggttgcgc gcgtcatcgc cgcgaacgat ctcgatgcga tgtatttcga gctggtctcc
    12721 cattggtcga atcttgttct tcaggctggt gacgccgagg ctccggtgac tgcgcaggcg
    12781 gcacgtttac caatcaagga tccggtggag cgcatgatgt atttcgacca gatctcgtat
    12841 ctgccggacg acatcctgac aaaggtggac cgggccagca tggcggtgtc gctggagtcg
    12901 cgcgaaccgc tcctcgacta tcgactcgtc gagtttgcat ggacgctacc gctgtcgatg
    12961 aaggtgcaaa acggagttgg gaaacgtgtg ctgcgccgcg ttctcgatcg ctacgtgccg
    13021 aaggagttga tcgaccgtcc caagatggga ttcggtattc cgctcgagtc gctgctccga
    13081 ggccgcctgc gcgactgggc ggaatcgctc ctcgatcctt ccacgatgcg cgcgcaggga
    13141 ttcctcgaca cggcgatggt gcaggcgcga tggaaggatc acctcgacgg caaagatcag
    13201 tggaagttct acttgtgggg tgtgctgatg tttcagcaat gggtaaggga caattgatac
    13261 gagggatggt gcgttcgacg actcgttgcc ccccatgact cagtgccgta ccgctctctg
    13321 gcggcttggg gtcgaacgtg cgtgacgagt attgacgtcc cggcgacggg gttcctcctg
    13381 tctgacagcc ccaaagatag aagatgtacc cgccgctact cgacaggtga cccggctttt
    13441 tcccgagaga catgagcgtc ggataccagc ctttgtagtc ggcgcatcca ccgctcatgt
    13501 cgaattcggc ccaggagcct gtaatctcac cctcgagacg tcagcttgcc gcggaatcct
    13561 ggaggagccg aatcgccaag ccgcaggtga gggtagaagc tcctgccaat gcgcccgccg
    13621 ctccatcttc tccttgctgc cagcgtgctg gcgcaatcgc cggacatctc gcagaagatg
    13681 cccgacgccc agcaagcgat cggggccggc gattgctgac gcgcgcaaac gaccctccgc
    13741 gagatcatcg ccgcgatcca caaactaccc tcgcgcacgc gatgtcggtg cacggcgatg
    13801 ctgaggtcga acacctgcgc gcctcgacga cagcgggagc gttcccacga ctcttcggct
    13861 cgtgcggaag cagttcacag agactccaat acctaattat tgctcgtggt tggatttagg
    13921 tgtaggctct ccttatatct tcgcatgctt cccatattgc ccctttctcc ttgaacaccc
    13981 ccatggcgaa acaagagtga actgaaatgt gctcaattgc gcaacgaaga ctcggcgcag
    14041 cctggatcgc tgcggggcga accgggattc cgcattcctt ccagtctgaa agcaggcacg
    14101 ctaatgaaca gatcaagggt attctcagta atcttctccc cactcgtcgc tcttctgggg
    14161 gcttgtacgt cagccatcac tagtacgccg accatcacca tctgtaaaca aactcagcca
    14221 ggcggcggcg caggatttca gttcactacg actaatggcg gtctcggttc gccgttcggt
    14281 ccgtttacgc tgaatgatac gcaatgccac acgatgttag tgaacgccgg ccaagaccac
    14341 tacaacgcat tcactgagaa tgtacctgct ggatggctgc tgacgaatat tagttgtaac
    14401 gccacgacca gcctcgtgac gattgttggt gctaacggca atcccgggtt tcaacccggt
    14461 gacaacacgg tgactatcga cttgaacgag ccgcacgtca cttgtacgtt cgtcaatgca
    14521 caaacgcctc actgttgtgg gttcaatctg gatctcagta ctggtcaggg caccgtcata
    14581 gatccattat ggacagttaa tggcggcaat gcctacgtta cgccgccgtc aaccgcatgg
    14641 actgttaaca tgccgcccgc gcaatggatt cagccggtgg cttcgccgac gccttccgga
    14701 aacgttcctc caggggttta caagtatgta gtctcattca caccgacgcc ttgcgccatg
    14761 ggtcacctcg agttgagtgg aacattcgcg gccgacaacg acgccggggc aatgctggac
    14821 ggcgtacaca ttggaacggc ttgcacgaac tgtttcaaca cggcggcagt tccgtttgcc
    14881 gttaacccac tcacactaac ttcgcacgtg ttggaaatta acgtgaacaa cttaggcggt
    14941 ttttcgggcc tgatcgtgaa tgctcacgtc aagagaatat gcccttagcg actcccttcg
    15001 ccgatgtctt ttgtccatta aggcctcagc gcgcaaacgc ccttgccacg actcgcatcg
    15061 gatatgcggc gaagtagccg aggttgcgga ggtcgtagcg aaggctcttg atcatgtgcc
    15121 tgaagaaccg ccgcggctga tgcaactgaa aatagcagcc ggccagcaca cgatggatgc
    15181 ggccgtacga atggttgcga agcgaccgta ctgccggatt ctgaaacgcc ctatcgagcg
    15241 cgcgcagcat tgagatctcc atttgtgcca cgttcagatg aatgccgcct ccgtgcaggc
    15301 gatacttgag cagtggttcg cgcacgtacc cgatgcggta acgaacggcg agtcgataac
    15361 agaagtccca atcctccgag ggttgcagcg ttttgtcgta cccgccgatc tcctgggcca
    15421 cccgtttcgg caccatgatg ctgctgccgg gcgctgtgat gacttcgcgg tcgaggcgca
    15481 ataactccgt cgctacccac ccttccatgc cgttctgcga tacgaaaagc agtttcccgt
    15541 cgcggtcgac ttgttcgaca gcgcagtgaa ccagaccgag gtcgggatcg gcgatgaaac
    15601 gcgcgatttc gtgctcgagc aattgcggct tcatgatgtc gtcggagtcg agaaaggaca
    15661 gatagtcgcc cttcgccgcc gcaatgcccg tatttcgcgc cgcgccgact ccgccgttgt
    15721 cctggtgaat gacccggatg cgctcgccgt actcagccag gatctgcggc gtgtcgtccg
    15781 tcgagccgtc atcgacgacg atgatctcca gcggacgata cgtttgcgcg agggcgctat
    15841 cgattgcatc gcgcaggaac cggccgtagt tgtatgtcgg aatgatgatc gagacggttt
    15901 tcacggatgc gcctttgtgc cacgagtgcg gtcggatggt aacaagctgt cagacatcgg
    15961 ttgaatctct gcgttgccgc ggaatgggca gcaatcgctg gaaagccttc cgctcgtcat
    16021 ccgtgaggac gcgcgtcagg atcaggaggg catatgcggc tactgccgca gcagcaacga
    16081 cggcgcgcac cattgcggac gtcgtgagta ttgtgacgat cggaacgggc agcagcagga
    16141 ccgccgctgc ggcgagatac gcccgtttgt tgtccggcca cggtgcgtac gcaaagccgg
    16201 acgtgcgttg ggcgaagtat gcaactgcga cagcgccggt cagcgtacga agactccatg
    16261 cgatcgctgc tccgaccgcg ccgaactgct ggatcaggac ggcggaaagg attacgtagg
    16321 ggatcaccag cgacaaattg aggcgggcga cgatgtcggt gcgcccaaga gcgatcagca
    16381 acgcgtaggg aacgtacgtc atgatctcgg cgacgatgcc gcccatgagc aggtacagcg
    16441 gcaatgtgct ctcgcgcccg aactccgcgc cggcccagat ggtgaagaac ggctgtgcca
    16501 ccgcgcagac gaacatcgcc gccggcacgg cccagaacag catgccgtgg agcgcgcgcc
    16561 ggtaaagcaa ctgcatttcc tcgcggccgg gccgcatctg gagctgcgag aaggcgggta
    16621 cgagtgattg gacgatcgtc gacggcaact gcgtcggcag gtaggccaga gtgaacgcga
    16681 ccgagtagaa cgccagcgcc tgtaatgacg caaagcgcgg gagcagcact ttctcggagc
    16741 tcgccagcag cgtggcggtc atcgaggaaa cgaccagcgc gccgccgaat cgggccagcg
    16801 gtttcaggag agcgcggtcg acgtgaggat gccgcaggct gggcaataac ttcagtccgg
    16861 cgatcgcgta cagcagcgcg gttgcgatgg cggcgcctgc aaccaccatc acggcggagg
    16921 tcagcccgcc gcccagcagc agaacgacgg gaatgagcag gatctgtccg gcagcggtgc
    16981 cggtgctgat gagagccacg agatccatgc gcagccgcac gaatgcaggt gtgttgaaga
    17041 ctcccgccac ggcccgggcg aaaaatccga ccgcggccag acggatggcc acaaccgcga
    17101 ccggtcgcaa cgacagagga aggtgcagac catgttcgac gagcaatggc gcaccaagca
    17161 ccagcaggat agccaacgtc gttgccggga ccaccgcgag ggcgagcgcc gtccagatcg
    17221 cggtcgcctc gccgcgatcg tcgccggcgg cgtatgcgtg ggaaccaaat cgcgtcgacg
    17281 ccgttcccat ccccaggtcg ccaaaaccga gatagccgat caggacatga acgagcgtga
    17341 ggacgccgta gctctgcgcg ccgaggaggc ggatggtgaa cggagtcgcc acgagcgtcg
    17401 ccagcatggt cacgccctgg ccgccgaagt tccagaagct gcctcgcacg acgcgtgagg
    17461 tcaggcctgg accattgcgt ggcgttggat acggcgcgcc cggaccagtg tcggcgagaa
    17521 gatcgccgtc gccgaacgcg agctgctcaa aaggttgagg atcacccatg ggaagcgagc
    17581 tcgggacatc gagcatgttc gttgtgcggc actgccagca cgttctggtt ttgctttttc
    17641 agctcggcgg aactgactgg tcgcagcatg ccccccgctt gcacttcgaa gagctggtag
    17701 ccaggccgct gcaaaccgac aaggaagtca gcggcatcgg tcatcccgaa gccgaaacca
    17761 tgcgcgctct ccggcccggc ttcgaagaga acgaccggcg cctcgacgcg atccagagtc
    17821 tccttcccgc cgcgaaagac cttcaactcc gccccttcca catcgcactt gatgaagttc
    17881 ggcacgggaa gctcgccctt gctcacgagg tcgtcgatgc gctgttgctg cacggtgatc
    17941 gtactggtct tgccgagggc cgcctgctga cgttgcgagg gcatcctctc caggctcgtc
    18001 caatccgcaa ggctgctcat cgtgcggtcc ggcggaacga agaagaccgc ctttgtgtcc
    18061 tcgtctgaca acgcgtaacg gctgagtgtg gcgttcccca acctctcgac gatgcgcgcc
    18121 aggcccgtga tcagttccgg gttcggctcg aaggcaaaga catgaccgcg ggaacccgcc
    18181 agttctgcga ggagcgcggt atgcactccg tagtttgcgc cgacgtcgaa gacggtatcg
    18241 cccggacgca cgaatcgacg catgaccgtc tgctcgtccc gctcgatcgg agaatggtcg
    18301 aagggcgttc cga
//
LOCUS       DATLVW010000426         5668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_62517, whole genome shotgun sequence.
ACCESSION   DATLVW010000426 DATLVW010000000
VERSION     DATLVW010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62517"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VNN25_12895"
     CDS             <1..521
                     /locus_tag="VNN25_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92472.1"
                     /translation="MGGMQALQWAVTFPDFMDKVISIVGTPKQTAYDLLLWKTELALI
                     DKPEGLKAAADINEMHLHTPAWIASHLEDVDKVMQSHEQALQKLDPFDYATLLRAMIG
                     IDIGPPDKIHAKMFIVVSLQDQMVNPAPARELARITKSPLLTLTGDCGHLATSCEQEI
                     LVREVTRFLDAR"
     gene            790..921
                     /locus_tag="VNN25_12900"
     CDS             790..921
                     /locus_tag="VNN25_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92473.1"
                     /translation="MRQPLEGIVAREEWVVASALRAAANYFDIHYKSIRFFRGGRVR"
     gene            1068..3470
                     /locus_tag="VNN25_12905"
     CDS             1068..3470
                     /locus_tag="VNN25_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXH92474.1"
                     /translation="MTTDHLDPVCGMTVDPARAAGSSTYRGQTIYFCGLHCKKKFDAN
                     PAAYAGDAEPIVAATAANYVCPMDPEVRQDHPGACPKCGMALEPEMVSAPAAKVEWTC
                     PMHPEVVRDAPGSCPICGMALEPRTVQAEEDNPELRDMTRRFWISVALTAPLVAFAML
                     RHLPGGGMFPHALMPWASWIELVLAAPVVLWCGWPFFERAWSSLVSRHLNMFTLIGLG
                     VTVAFLYSLFATLLPNLFPSGLRDHAGNVGVYYEAAAAIVALVLLGQVMELRARSRTG
                     VAIRALLGLAPTTARRIDADGTEHDVALDQVVAGDLLRVRPGEKVPVDGVVVEGSSNI
                     DESMVSGEPLPVAKRAGDTVIGATVNGTGSLVMRAEHVGADTLLSRIVNMVAQAQRSR
                     APIQRLADQVSGWFVPAVIGIAIMTFVVWAIAGPNPRLVYALVNAVAVLIIACPCALG
                     LATPMSIMVAVGKGATAGVLFRDAEAIEVLRTVDTLVIDKTGTLTAGKPEVVTVIATT
                     GFDENDLLRLGASVERASEHPLAAAIVTAAERRGAKVTAATEVESISGKGVRGLAGGK
                     RVALGNRALLEQLGVDAATLADRAESLRVDGQTVMFVVVDGAIAGLIGVADPMKETTH
                     EAIKALHAEGLRIVMLTGDNKTTARAVAAKLGIDEVIAEVLPDQKAEAVKRLQREGRI
                     VAMAGDGINDAPALAQAAVGIAMGTGTDVAMESAGVTLVSGDLRGIVRALRLSNATMR
                     NIRQNLFFAFVYNAIGVPVAAGVLYPFFGILLSPMIAAAAMSFSSVSVITNALRLRNA
                     RI"
     gene            complement(3565..3939)
                     /locus_tag="VNN25_12910"
     CDS             complement(3565..3939)
                     /locus_tag="VNN25_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH92475.1"
                     /translation="MDTKQVGEKLVALCREGRNLDAVNTLYSPDIVSVEAMQTGPMPA
                     EQRGIEAIRGKNKWWIDNHEVHSASTDGPYPNGDRFTVKYHYEITPKTGPASGQRIAM
                     DEVALYTVANGKIVKEEFFYAM"
     gene            4046..>5668
                     /locus_tag="VNN25_12915"
     CDS             4046..>5668
                     /locus_tag="VNN25_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXH92476.1"
                     /translation="MPSDLPAETLSENWFDRQSERVRRIAKRFLLLTIVLIGALCGIL
                     AVLFHRFVEAARALLIGRALNTSEPWRSVLIVITPALVFALLAWCIRRLAPRAVGANL
                     ARVRIAYNRNPEALGWRSIVATFIATPLSLGAGAPLGPEGPIVVITSGTSAAIARILR
                     LPRRLVRGMIPVGVAAGIAAIFNTPITGVVFALEEVFGSAERDLLGGVIVGAVSAAVV
                     ERSLLGGQPILAAPFANWGDARELLGFALIGILAGVVSGYAITLTHRLKRLGARAMPS
                     MPLRAAIAGMSIGALGLLAPSILGVGYDSVSLWLHGGGTAEQTALAFGAKTIAFIIAI
                     SAGVLGGTFAPSLFMGAALGAAIGHLAQKVFPGAGIDPRAYALLGMGSFFAGLLRSPI
                     SAVLIVVELTRDYELMIPLMLAVSLSVMVSRRISRNSMVEQQMIDEGYIEAKESSDPL
                     ANVRVSEAMQANPTTVSADVTLRDAARGVAGMHHRIYPVVDAEGRFAGVLSRESIESA
                     ARENTLDSSVLASIEQPKLIATAGELLIDLVQR"
BASE COUNT          939 a         1800 c         1852 g         1077 t
ORIGIN      
        1 cgatgggagg gatgcaggcg ctgcagtggg cggtcacgtt tcccgacttc atggacaaag
       61 tcatctcgat cgtcggaacg ccgaagcaga cggcgtacga cctgctgctc tggaaaacag
      121 agctcgcgct gatcgacaag ccggagggat tgaaggccgc ggccgacatc aatgaaatgc
      181 acctgcacac gcccgcctgg atcgccagcc atctggagga cgtcgacaaa gtgatgcagt
      241 cgcacgagca ggcgctgcag aaactcgatc ccttcgatta cgccacgctg ctgcgggcga
      301 tgatcgggat cgacatcggg ccgccagaca agatccacgc gaagatgttc atcgttgtct
      361 cgctgcagga tcagatggtg aatccggcgc cggcacgcga gctggcgcgc atcacgaagt
      421 ctcccctgct cacactcacc ggcgactgcg gccatctcgc tacgtcctgc gaacaggaga
      481 ttctcgtgcg cgaggtgacg cgctttctcg acgcccgctg atccgcgggc gatcgatcgc
      541 caacctcgga tgttgcattc aacacaagag gccgctacta ttcgctgcac agcaacatgt
      601 gcagtacgaa aggccgggtg ctccccggcc tttttgtctt tgcacgcatt tcgggaggga
      661 ggggttgcga tgatgtcggg gcaacagatc gcgaagcttg cgaaacgaag aagctgtcca
      721 cgctgcagtc tggaaaaaac cgaggggaat gcgctctgcc ggcggtgccg gttcaaactt
      781 ccgccgcaca tgcggcagcc actggaggga atcgtcgcgc gcgaagaatg ggtggtggcc
      841 agcgcgctcc gcgccgcggc aaactacttc gacatccact acaagtcgat ccggttcttt
      901 cggggcgggc gagtgcgctg aaacgggcca gctcgtttct tagaatcggt tcacgcggag
      961 acgcggagaa cgcgggggaa accagtgctt ctctccgcgc ctccgcgtct ccgcgtgacc
     1021 ggtcccccct ttgcttgacc ggtcgggtag agtccgccca acaaccaatg accaccgacc
     1081 atctcgatcc tgtttgcggc atgaccgtcg atcctgcgcg tgccgccggc tcctcgactt
     1141 accgcgggca gacgatttac ttctgcgggc tccattgcaa aaagaagttt gacgcgaatc
     1201 ctgcggcgta cgcgggcgat gcggagccga ttgttgcggc cacagcggca aactacgtct
     1261 gcccgatgga tcccgaggtg cggcaggatc atcccggggc gtgcccgaag tgcgggatgg
     1321 cgctggagcc ggaaatggtt tccgcgccgg cggcgaaggt tgagtggacc tgtcccatgc
     1381 atcccgaggt tgtgcgcgat gcgccgggga gttgcccgat ctgcgggatg gccctcgagc
     1441 cccgcacggt gcaggccgag gaagacaatc ccgagctgcg cgacatgacg cggcgattct
     1501 ggatcagcgt tgcgctcacc gcgccgctcg tcgccttcgc catgctgcgg catctgccgg
     1561 ggggcggcat gttcccgcac gcgttgatgc cgtgggcttc ctggatcgag ctcgtgttgg
     1621 ccgctccggt cgtgctctgg tgcggctggc cgtttttcga gcgcgcctgg agctcgctcg
     1681 tctcgcgcca tctcaacatg ttcacgttga tcggcctcgg cgtgacggtg gcgttccttt
     1741 acagcctttt cgcgacgctg ctgccgaacc tctttccgag cggactgcgc gatcatgccg
     1801 gcaacgtcgg cgtctactac gaagccgctg ccgcgatcgt cgccctcgtc ctcctcggcc
     1861 aggtgatgga acttcgcgcg cgaagccgca ccggcgttgc gattcgcgct ctcctcgggc
     1921 tcgcgcccac gacggcgcgg cggatcgatg cggatggaac cgagcacgat gtcgccctcg
     1981 accaggtggt cgccggcgat ctgctgcggg tgcggccggg cgagaaggtg ccggtcgacg
     2041 gtgtcgtcgt cgaaggatcg agcaacatcg acgaatcgat ggtctcgggc gaaccgttgc
     2101 ctgtggcgaa aagggcgggc gataccgtga tcggcgcgac ggtgaacggc accggatcgc
     2161 tcgtcatgcg cgcggaacac gtcggtgcgg acacgcttct ttcgcggatc gtaaacatgg
     2221 tggcgcaggc gcaacggagc cgcgcgccga ttcagaggct cgccgatcag gtgtctggat
     2281 ggtttgtccc ggccgtgatc ggcatcgcga tcatgacatt cgtcgtgtgg gcgatcgccg
     2341 ggccgaaccc gcggctcgtc tacgcgctcg tcaacgccgt cgcggtgctg atcatcgcct
     2401 gcccctgcgc cctcgggctg gccacaccga tgtcgatcat ggtggctgtg ggaaaagggg
     2461 cgacggcagg cgtgctgttc cgcgacgccg aggcgatcga ggtgctgcgc acggtcgaca
     2521 cgctcgtcat cgacaagact ggaacactga cggccggcaa gccggaggtg gtcacggtca
     2581 tcgctacaac cggtttcgac gagaacgatc tgcttcgcct cggcgcgagc gtcgagcgcg
     2641 ccagcgaaca tccgctggcg gcggcgatcg tgaccgcagc ggaacgccga ggcgcgaagg
     2701 tgaccgcggc gacggaggtc gaatcgatca gcggaaaggg tgtccgaggt ttggccggcg
     2761 gaaagcgcgt ggccctcggc aatcgtgcgc tcttggagca gctaggcgtc gatgccgcga
     2821 cgctcgccga tcgcgccgag tcgttgcgcg tcgacggcca gacggtgatg ttcgtcgtcg
     2881 tcgacggcgc aatcgcggga ctcatcggag tcgctgatcc gatgaaagag accacgcacg
     2941 aagcgatcaa agcactgcat gccgaggggc tgcgcatcgt gatgctcacc ggcgacaaca
     3001 agacgacggc ccgcgcggtc gcggcgaagc tcggcatcga cgaagtgatc gccgaggtgt
     3061 tgccggatca gaaggcggag gcggtgaagc ggctgcagcg ggaaggacgc atcgtggcga
     3121 tggctggcga cggtatcaac gacgctcccg cgctggcgca ggccgcggtc ggcatcgcca
     3181 tgggaacagg aacggacgtg gcgatggaga gcgcgggcgt aacgctcgtg agcggcgatc
     3241 ttcgcggcat cgttcgcgcg ctgcgcctca gcaacgcaac gatgcgcaac atcaggcaga
     3301 acctcttctt cgcattcgtc tacaacgcca tcggcgtgcc ggttgcggcc ggcgtgttat
     3361 atccgttctt cggcatcctg ctcagcccga tgatcgccgc ggcggcgatg agcttcagct
     3421 ccgtgtcggt gattacgaat gcgctgcggt tgcggaacgc gaggatttag cgaaaaacgg
     3481 cttcaccaca gagacacaga ggtcgcagag ttacagtgtc aactctgtgt cctcagtgtc
     3541 tcggtggtga ttttttctgc cgccctacat cgcgtagaag aactcttcct tcacgatctt
     3601 tccgttggcg accgtgtaga gcgcgacctc gtccatcgcg atccgctggc cgctggccgg
     3661 accggtcttc ggggtgatct cgtagtggta cttgaccgtg aagcggtcgc cgttcgggta
     3721 cggtccgtca gtggacgccg agtgaacctc gtggttgtcg atccaccact tattcttgcc
     3781 acggatcgcc tcaatcccgc gctgctcggc cggcatcggc ccagtctgca tcgcctccac
     3841 gctgacgatg tcggggctgt agagcgtgtt caccgcgtcg aggttgcggc cctcgcggca
     3901 aagagcgacc agcttttctc caacctgttt cgtatccatt tgaacctcct ccaatgcgac
     3961 aatcaaatca gatgcccagg cgcgcccgtg gcaatttgat gtgaattccg cgcatgtggc
     4021 acgttcgacg aaaccgaatc catccatgcc gtctgatctc ccagccgaaa cactttccga
     4081 aaactggttc gatcgccagt cggaacgtgt acggcgcatt gcgaagcggt ttctgctgct
     4141 gaccatcgtt ctcatcggcg ccctttgcgg catcctggct gtgctctttc accgattcgt
     4201 cgaagccgcg cgcgctctat tgattggcag ggcgctcaac acgagcgagc cgtggcgctc
     4261 ggtgctcatc gtgatcacgc ctgcgctcgt cttcgcgctg ctggcctggt gcatccgccg
     4321 tcttgcgccg cgcgccgtcg gcgcgaatct ggctcgcgtc cggatcgcct acaacaggaa
     4381 tcccgaggcg cttgggtggc gcagtattgt ggcgacgttt atcgcgacgc cgctctcgct
     4441 cggcgcgggc gcgccgctcg ggccggaagg gccgatcgtc gtgatcacca gtggcacttc
     4501 cgccgccatt gctcgcattt tgcggctgcc gcggcgtctc gtgcgcggca tgatcccggt
     4561 tggcgtcgct gccggcatcg ccgcgatttt caacacgccg atcaccggag tggtcttcgc
     4621 gctcgaagaa gtcttcggtt ccgcggagcg agatctcctc ggcggcgtga tcgtcggagc
     4681 ggtttcggcc gcagtcgtgg agcgctcgct cctcggcggg cagccgattc tcgccgctcc
     4741 gttcgcgaac tggggcgatg cgcgcgagct gctcggcttc gctctcatcg gcattctcgc
     4801 aggtgtcgtc tccggttacg cgatcacgct cacgcaccgg ctcaagcggc taggggcgcg
     4861 ggcgatgcca tcgatgcctc ttcgcgccgc gatcgccgga atgtcgatcg gcgccctcgg
     4921 tcttctcgct ccctcgatcc ttggcgtcgg ctacgactcg gtttccttat ggcttcatgg
     4981 cggcggaacg gcggaacaga ctgcgctcgc attcggcgcg aagacgatcg ccttcatcat
     5041 cgccatcagc gcgggagtcc tcggcggaac cttcgcgcca tcgttgttca tgggcgctgc
     5101 gctcggcgcg gccatcggtc atctggcgca gaaggtcttc cccggcgccg gaatcgatcc
     5161 acgcgcctat gccctcctcg gcatgggctc cttcttcgcc ggcttgctgc gtagtccgat
     5221 ctccgcggtg ctgatcgtcg tcgagctcac gcgagactac gagctgatga ttccactcat
     5281 gctcgcggtc tcgctatccg tgatggtttc gcgccgcatc tcccggaaca gcatggtcga
     5341 gcagcagatg atcgacgagg gctacatcga agcgaaggag tcttccgatc cgctcgccaa
     5401 cgtgcgcgtg tccgaagcga tgcaggcgaa tcccacgacc gtctccgcgg acgtgacgct
     5461 gcgcgatgcg gcgcgcggcg tcgcgggaat gcatcaccgt atctatccgg tcgtggatgc
     5521 cgaggggaga ttcgccggcg tactgtcacg tgagtcgatc gagagcgcgg cgcgcgagaa
     5581 cacactcgac agctccgtac tcgccagcat cgagcaacca aagctgatcg ccaccgctgg
     5641 cgagctgctc atcgatctcg tgcagcgc
//
LOCUS       DATLVW010000427         8958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_429015, whole genome shotgun sequence.
ACCESSION   DATLVW010000427 DATLVW010000000
VERSION     DATLVW010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429015"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VNN25_12920"
     CDS             <1..308
                     /locus_tag="VNN25_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92477.1"
                     /translation="HLQPMVYFGRTMDAETGRGIGIVDEVISAEQLPVRAQIIAEQLK
                     SIPRHTFRLTKRQLREPYLRDAANIATASADEIDAIWSDPATHAHIREYLAMTIGKR"
     gene            569..1162
                     /gene="mug"
                     /locus_tag="VNN25_12925"
     CDS             569..1162
                     /gene="mug"
                     /locus_tag="VNN25_12925"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HXH92478.1"
                     /translation="MALLSPRGTTKPTKEQLLAAAGTTLPDVIARDLDVLFCGINPGL
                     YTAAVQQHFGRPGNRFWPTLYRSGFTPRLFHPSEQRELLQHGAGITNVVARATAAAAE
                     LSAEELVDGAKTLARKVGRYWPRVLAIVGIGAYRTGFARPQAKSGLQDETIGNTRIWI
                     LPNPSGLNANYRPEDLAALYRELREWIRSADDADGRR"
     gene            complement(1175..2326)
                     /locus_tag="VNN25_12930"
     CDS             complement(1175..2326)
                     /locus_tag="VNN25_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH92479.1"
                     /translation="MSVVGSRVSEIEATIPLPAPTPDPRNPTPHLFGMLPVDLAAHLR
                     ASGVIVRDAEARRVLAHAIAHGREGFPSARPVPRAVEEAVERMTTRSRLEIIERATDT
                     NDGFVKYLFRLHDGALAEAVRIPLEVPGRFTVCISSQAGCAMGCTFCATGRMGLIRNL
                     EPWEIVAQWIAVRDEADGIVTGALFQGQGEPLHNYASVIRAAEILSHPCGGRIAAKNI
                     TISTVGLVPQILRFTRERHPYRLIISLTSAIDTRRRMLLPSAAAWPVRELADAMRAYQ
                     SSSNSRVTIAWVVLGGINTGNDEVEAFCALFGDMPIRLNLIDVNDARPDGFHRASDEE
                     LATFRDALRSLGAPVVRRYSGGAARHAACGMLAAMRSEIDPQMTQMNTD"
     gene            complement(2334..2729)
                     /locus_tag="VNN25_12935"
     CDS             complement(2334..2729)
                     /locus_tag="VNN25_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92480.1"
                     /translation="MSDDTPKAQRFDLLSNSESGLSYVREKRGGTMRVLTESELADFN
                     DPPPCPQCAEQFGCEHFNCAGEEMLDDDAIVAHAPPEWLAFAKSAGISLQDLERLKLI
                     REHEGEYRVVEEAVTDMRTLEIVLLLNGE"
     gene            2926..6042
                     /locus_tag="VNN25_12940"
     CDS             2926..6042
                     /locus_tag="VNN25_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HXH92481.1"
                     /translation="MQKTIRAALVVLSLALPLAAKIDPGMYGDIRWRMIGPFRGGRTV
                     AAVGVPQQPNVFYIGVNNGGVWRTNDYGRIWTPLFDDQPSGSIGALAVAPSDPNVVYA
                     GSGEGLQRPDLATGDGMYRSSDAGKTWTHLGLRDAQQIPQIVIDPADPNRLFVAVLGH
                     PYGPNEERGIFRSTDGGKSFQKVLYKNEFTGGIDVILDPSNAQTVYAALWQAQQGPWE
                     NAYLDGPATGLFKSTDGGTTWSELKSGLPESKVMGRVGIEVSKSDPKRLYVICGVDAK
                     SGGLYRSNDGGATWTRANTDERIWGRPGDFNEVRSDPKNADIVYVANVVTWKSTDGGK
                     TFASFRGAPGGDDYHRVWINPNDSKTILIASDQGAIVTVNGGESWSSWYNQPTAQMFH
                     VNADNAFPYHVCGGQQESGSACVASRGDDGAITFREWHPVGVEEYGYAVPDPLDPNII
                     YGGKISRTDRRTGQVKDITPKPLRKGGYRTVRTAPVVFSLADPHALFFASNTVWKTTN
                     GGESWTEISPDLSRKTWTLPESARGYANTPQTKVTQRGVVYALAPSPLDINTLWAGTD
                     DGLIHVTRDSGKTWTDVTPSELKPWAKVSILEASHSDANEAYAAINTIRLDELHPHIY
                     RTRDGGKTWQHIVSGIADGATINVVREDPKHRGLLFAGSETQVWVSVNDGDSWQSLRQ
                     NMPPSSIRDLVVHDDDVIAGTHGRGIWILDDIEPFRQMNARLSDATLFKPQHAIRTRW
                     NKYTDTPLPPDEPMGQNPPDGAIIDYYLPSAAAGPVTLEILDKSGAVIRRYASTDPAP
                     APKDEGNIPWYWIRPLQSPSAAAGMHRFTWDLHYPPAPGSRNAYPIAAVPHDTAPAPT
                     SPWVLPGDGYTVRLTVNGKTFSQPLTVAMDPRVKTSQADLEQQFALSKEMYDDIAQLQ
                     AASERIGVVRGQLRAAKPASDAIKALRKQARELSGVPSEDDDEGPPSAAPNGETVNSL
                     LQSLRAMERLLQQSDEAPTTQALAAVEDRRAAVAEVLGRTKAFETAAQAAKLPPPAKG
                     SSAIKPQP"
     gene            6201..8309
                     /locus_tag="VNN25_12945"
     CDS             6201..8309
                     /locus_tag="VNN25_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXH92482.1"
                     /translation="MRPKVLVLSALLFALLSAACGRRAERPSIILISIDTLRADHLHA
                     YGYRGVATPNIDALARDGVIYQNAWSHCPLTLPSHLSMLTGLLPPEHGVRDNAGYRFD
                     GKAHRTLASLLQDNGYATGAAVSAYVLRATTGANAGFDDYDDAIGVVDGAPLGALQRS
                     GRTTEAIAERWIDAHQGKPFFYFLHIYEPHTPYQPTYDADVAASDAIAGSLLAHLKQR
                     GIYDDALIILTSDHGEGLMDHGEAEHGVFLYREDLRVPLIVKLPRETSHGRVAALAQL
                     IDIAPTVLDAAGVVPPSAMHGMSLLRGSGVGGRGSEEKSASSDSRTSYAESFYPRIHL
                     GWSELRSVVRGTLHVIDAPKPEIYDLRADTGERNNVADRERRAFAELRDVARATSGSF
                     AAPERVDAEEAKKLAALGYVSAGSDAGNGPLPDPKDVIGQLATLKSAHSIDDLQRLLA
                     ANPRWSDVREQLGGELGRVGDHASAARVFEEGIRMAPRLAGTFALSAGFELLTMHDVA
                     NASTHAQLALSAGEPGAHLLLGEIALVQRDLATATREETAAEEMASQKAHALFLAARI
                     AAMQHDYPRTLRILDEVQRTRATTGESLPEHFHYVAGDALARTNRPAEAENEMVREIA
                     SDPHDLRAYVDLSLLQFIGGQRDDAEITLAKLAVMHPDTPLSAEVARELATFGDAAGS
                     REWEHRLHDVHGSLTDPAGH"
     gene            8481..>8958
                     /locus_tag="VNN25_12950"
     CDS             8481..>8958
                     /locus_tag="VNN25_12950"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92483.1"
                     /translation="MKKLISLAALLITTVAFAKDPAANSKIKIQDQDSKGVPTFVTGE
                     LGSLGAGASDQAVKAFLKSQKDLLQMAGTEDFDVKSSQRDALGQSHVKFQEKLKGLPV
                     VGAEYIVHSDSNGNVFAINGRFVADRDLPRNPSIDSVMAIRTAATQLGAWDADIVDV"
BASE COUNT         1680 a         2825 c         2877 g         1576 t
ORIGIN      
        1 agcacctgca gccgatggtc tatttcgggc ggacgatgga cgcggagacg ggacgtggca
       61 tcggcatcgt cgatgaggtg atctccgccg agcagcttcc agtccgcgcg caaatcatcg
      121 ccgagcaatt gaagtcgatt ccgcggcaca cgttccgatt gacgaagcgc cagcttcgcg
      181 aaccgtacct tcgcgatgca gcgaatatcg ctaccgcgtc cgccgacgag atcgacgcga
      241 tctggtccga tccggcgaca cacgcgcaca tccgcgagta tctggcgatg acgattggga
      301 agcgctaacg gctgtcatcc ggatgacatc catcgtgtca tcctgagcct gccggaggcg
      361 cccgcgcgac tcgcgccctc acgcagggcg ggacggcgcc gcaggacggc gaaggatctc
      421 aagttgccgc gcacatggca tgcgcatcgt aatttgagat ccttcgatcg tcctccgccg
      481 caatgcctgc cagttgtcag tgcagcgtga aggcgcggct ccggcgactc aggatgacac
      541 cgcgcaacta ggctacgctg cgtattcgat ggctctactc tcaccacgag gcaccacaaa
      601 accgacaaag gaacagctcc ttgcagcagc cggcacgacg ctgcctgatg tgatcgcgcg
      661 cgatctcgac gtgctgtttt gcggcatcaa tcccgggctc tacacggcgg cggtgcagca
      721 gcatttcgga cggccgggta atcgattctg gccgacgctt tatcggtcgg ggttcacgcc
      781 gcggctcttt catccgtcgg aacaacgcga gttgctgcag cacggcgcgg gaatcacgaa
      841 cgtcgtggcg cgcgcaacgg ctgcggcggc ggagttgagc gctgaggagt tggttgacgg
      901 cgcgaagact cttgcgcgca aagtggggcg ctactggccg cgggtgctcg caatcgtcgg
      961 catcggtgcg tatcgaacgg ggtttgcacg tccgcaagcg aagagcgggt tgcaggacga
     1021 gacgatcggg aacacgcgca tctggattct gccgaatccg agcgggctga atgcgaacta
     1081 tcggccggag gatctggctg cgctgtatcg ggagttgagg gagtggatcc gatccgcaga
     1141 tgacgcagat ggacgcagat agcagataga tgaatcaatc tgtgttcatc tgcgtcatct
     1201 gcggatcaat ctcgctcctc atcgcagcga gcatcccgca ggcggcatgt ctcgcggcgc
     1261 cgccggagta gcggcgaacg actggggcgc cgagggagcg gagcgcatcg cgaaatgtcg
     1321 ctaactcttc gtctgaggcg cggtgaaaac catcggggcg ggcgtcgttg acgtcgatca
     1381 agttgaggcg gatcggcata tcgccgaaga gcgcgcagaa cgcttcgact tcatcgttgc
     1441 cggtgttgat gccgccgagg acgacccagg cgatcgttac gcgcgaattg ctcgatgatt
     1501 gataggcgcg catggcatcg gcgagctcac gcactggcca ggccgccgcg gaggggagca
     1561 acatcctgcg ccgggtgtcg atggccgagg tgagggagat gatcaggcgg taagggtgac
     1621 gctcgcgggt gaagcggagg atctgcggaa cgaggccgac tgtggagatc gtgatgttct
     1681 tcgcggcgat gcggccgccg caaggatgcg agaggatctc cgccgcgcgg atgacggacg
     1741 cgtagttgtg caacggctcg ccctgtccct ggaagagcgc gccggtgacg atgccatctg
     1801 cttcgtcgcg cacggcgatc cattgcgcga cgatctccca cggctcgagg ttgcggatca
     1861 atcccatgcg tcccgtcgca cagaaggtgc atcccatcgc acagccggcc tgactggaga
     1921 tgcacaccgt aaatcgtccc ggcacctcca gcggaatcct caccgcctcg gcgagcgcac
     1981 catcgtgcag acggaagagg tatttgacga agccgtcatt cgtgtctgtt gcccgctcga
     2041 tgatctcgag gcggctgcgt gtcgtcatcc gctcgaccgc ttcttcgacg gcgcgcggaa
     2101 cgggccgtgc tgacggaaag ccttctcgtc cgtgcgcgat cgcatgcgcg agcacgcgcc
     2161 tcgcctcggc gtcgcgaacg atcacgccac tggcacgaag atgcgcggcg agatcgacgg
     2221 gcagcatgcc gaaaagatgg ggtgtcgggt ttcgggggtc gggagtcgga gcaggcaatg
     2281 gaatcgtcgc ttcaatctcc gacacccgac tcccgacgac cgacaccccc gcctcactcc
     2341 ccgttcagca gcagcacgat ctcgagcgtc cgcatgtcgg ttacggcttc ttcgacaacg
     2401 cgatactcgc cctcgtgctc gcgaatcagc ttgaggcgct cgaggtcctg caggctgatg
     2461 ccagcggatt tggcgaaggc aagccactcc ggcggagcgt gggcgacgat ggcgtcgtca
     2521 tccagcatct cttcgccggc gcagttgaaa tgctcgcatc cgaactgctc ggcacattgc
     2581 gggcagggcg gcggatcgtt gaagtccgca agctcgctct cggtgagcac gcgcatcgtt
     2641 ccgccgcgct tctcgcgaac gtaactgaga ccgctctcgc tgttggaaag aaggtcgaag
     2701 cgctgggctt tgggtgtgtc gtcggacatg aatgtcagat cgtaccgcag cggggtttct
     2761 cacgcgaagg cgcgaagacg cgaaggagaa ccgtcaaacc ccttcgcgtc ttcgcgtctt
     2821 cgcgtcttcg cgtcttcgcg tgaccacgac aatcaacgtt gacaacattg agcgcgcttt
     2881 cgctgccgcc tcgccaaccg aaacctgcaa ctacaatatc cggccatgca gaaaacgatt
     2941 cgtgccgctc tcgttgtcct ctcgctcgct ctccctctcg ccgcgaagat cgatcccgga
     3001 atgtatggcg acatacgctg gcgcatgatc gggccgttcc gcggggggcg caccgtcgcg
     3061 gcggttggtg tgccgcagca gccgaacgtg ttctatatcg gcgtcaacaa cggcggggtc
     3121 tggcggacga acgactacgg ccgcatctgg acgcctctct tcgatgatca gccttccggc
     3181 tcgatcggcg cgctggcagt ggcgccgtcc gatccgaacg tcgtctacgc aggcagcggc
     3241 gaaggcttgc agcgacccga tctcgccacc ggcgacggca tgtaccgttc ttccgacgcc
     3301 ggcaaaacat ggacacacct cgggctccgc gacgcgcagc agattccgca gatcgtcatc
     3361 gatcccgcag atccgaaccg gctcttcgtg gccgtactcg ggcatccgta cggtccgaat
     3421 gaggagcgcg gcatcttccg ctcgaccgac ggcggcaagt cgtttcagaa agtcctctac
     3481 aaaaacgaat tcaccggcgg catcgacgtc atcctcgatc cgtcgaacgc gcagaccgtc
     3541 tacgccgcgc tctggcaggc gcagcaaggt ccgtgggaga acgcctatct cgacggtccg
     3601 gcgaccggct tgttcaagtc gaccgacggc gggacgacgt ggagcgagtt gaagagtggc
     3661 ctgcccgagt cgaaggtgat gggacgcgtc gggatcgaag tgtcgaagtc cgatccgaag
     3721 cggctctacg tcatctgcgg tgtcgatgcg aagtcgggcg gcctctatcg ttcgaatgac
     3781 ggcggcgcga cgtggacgcg tgcaaacaca gacgagcgca tctggggacg ccccggcgat
     3841 ttcaacgaag tgcgcagcga tccgaaaaac gccgacatcg tttacgtcgc caatgtcgtg
     3901 acgtggaagt caaccgacgg cggcaagacc ttcgcttcgt tccgcggcgc gcccggcggc
     3961 gacgactacc accgcgtctg gatcaacccg aatgattcga agacgatcct catcgcctcg
     4021 gatcagggag cgatcgtcac cgtcaacggc ggcgagtcat ggagctcctg gtacaaccag
     4081 ccgaccgcgc agatgtttca cgtcaacgcc gacaacgcct tcccctatca cgtgtgcggc
     4141 gggcagcagg aaagcggctc ggcgtgcgtg gcaagccgcg gggacgatgg cgcgatcacg
     4201 ttccgcgagt ggcatccggt gggcgtcgaa gagtacggct acgcggtccc cgatccgctc
     4261 gatccgaaca tcatctacgg cggcaagatc tcacgcacgg accggcgcac cggccaggtg
     4321 aaagacatca cgccgaagcc gcttcgcaaa ggcggctacc gaaccgtgcg tacggcgccg
     4381 gttgtcttct cacttgccga tccgcacgcg ctcttctttg cgtcgaacac ggtctggaaa
     4441 acgaccaacg gcggcgagag ttggacggag atcagtccgg acctttcgcg caagacgtgg
     4501 accttgccgg aaagcgcgcg cggttatgcg aatacgccgc agacgaaagt gacgcagcgc
     4561 ggcgtggtct atgcgctcgc gccgtcgccg ctcgacatca acacgctgtg ggcgggcacc
     4621 gacgacggcc tgattcacgt cacgcgcgac agcggaaaaa cgtggacgga cgtgacgccg
     4681 tcggagctca agccgtgggc aaaggtatcg atcctcgagg cgtcgcacag cgacgcgaac
     4741 gaagcgtacg ccgcgatcaa cacgatccgt ctcgacgagc ttcatccgca catttaccgg
     4801 acgcgcgacg gcggcaagac gtggcagcac atcgtcagcg gcatcgcgga cggcgcgacg
     4861 atcaacgtcg tgcgcgaaga tccgaaacac cgcggtctgc tctttgccgg aagcgagacg
     4921 caggtctggg tctcggtcaa cgacggcgat tcatggcagt cgctgcgcca gaacatgccg
     4981 ccgtcgtcga tccgcgatct cgtcgttcat gacgatgacg tcatcgccgg cacgcatgga
     5041 cgcggcatct ggatcctcga cgacatcgag ccgttccggc agatgaacgc gcgcttatcc
     5101 gacgcgacgc tcttcaaacc gcagcacgcg attcgaacgc gttggaacaa gtacaccgac
     5161 acgccgctgc cgcccgacga gccgatgggg cagaacccgc cggacggcgc catcattgat
     5221 tactacctgc cgtctgccgc ggccggcccg gtgacgctgg agattctgga caagtcgggc
     5281 gccgtgatcc gccgttacgc gagcaccgat ccggcgcccg cgccgaagga cgaaggcaac
     5341 atcccctggt attggatccg tccgctgcaa tcgccatccg ccgcggcggg catgcaccgc
     5401 ttcacgtggg atctgcacta cccgcccgcg ccgggctcac gcaacgccta tccgatcgcc
     5461 gccgttccgc atgacaccgc gcccgcgccg acttcgccgt gggtgcttcc gggcgatggc
     5521 tacaccgttc gattgaccgt caacggcaaa acgttctcgc agccgctgac ggtggcgatg
     5581 gatccgcgcg tcaagacatc gcaggcggat ctcgagcagc agttcgcgct gtcaaaggag
     5641 atgtacgacg acatcgcgca actgcaggcg gccagcgaac gcatcggcgt ggttcgcggt
     5701 caacttcgcg cggcaaagcc ggccagtgac gccatcaagg cgttgcgcaa acaggcgcgc
     5761 gagctttcgg gtgtgcctag tgaagacgac gatgaaggtc cgccgtccgc cgcgccgaac
     5821 ggcgagacgg tgaacagcct tctgcaatcg ctgcgggcaa tggagcgtct gctgcagcaa
     5881 tccgacgagg cgccgacgac gcaagcgctc gcggctgtcg aagatcgccg cgctgcggtt
     5941 gccgaggtgc tggggcggac gaaggcgttc gagacggccg cgcaggctgc gaagctgccg
     6001 ccgccggcga aaggatcgag cgcgatcaag ccgcaaccgt gaagattagc cgcccagact
     6061 gcatctcctc tttgcgttgc aacgaagatg tcgtgcttgt aacgcccccc tcatccggct
     6121 tcgccacctt ctccccccga aaagcacggg gggagaaggc gctcgattgg aagggcttgc
     6181 gctttggcaa ggtgtgagaa atgcggccta aagtactcgt tctttctgcg ctgctgttcg
     6241 ctctgctctc cgcggcatgc gggaggcgcg cggagcgccc ctcaatcatt ctcatctcga
     6301 tcgatacgct ccgcgcggat catctccacg cgtacggtta tcgcggcgtc gctacgccga
     6361 acattgacgc gttggcgcgc gacggggtca tctaccagaa cgcctggtcg cactgtccgc
     6421 tgacgcttcc gtcgcatctt tcgatgctca ctgggctctt gccgccggag catggcgtgc
     6481 gggacaacgc cggctatcgc ttcgacggca aggcccaccg cacgctcgcg tcgctgctgc
     6541 aggataacgg atacgcgacc ggcgcggctg tgtcggcgta cgtgctgcgt gcgactaccg
     6601 gtgcgaatgc cggtttcgac gactacgacg acgcgatcgg tgtcgtcgac ggcgctcccc
     6661 tcggcgcgct gcaacgctcc ggccgcacca cggaagcgat cgcggagcgg tggatcgacg
     6721 cgcatcaggg aaagccattc ttctacttcc ttcacatcta cgagccgcat acgccgtatc
     6781 aaccgacgta cgacgccgat gtcgccgcca gcgatgccat cgccggatcg cttctggcgc
     6841 atctcaaaca gcggggcatc tacgacgatg cgctgatcat cctcacttcc gatcacggcg
     6901 aagggctgat ggatcacggc gaggcggagc acggcgtctt tctctatcgc gaggatttgc
     6961 gtgtgccgct gatcgtgaag cttccgcgcg agacttcgca tggccgagtc gcggcactgg
     7021 cgcagttgat cgatatcgcg ccgacggtgt tggatgccgc gggcgtggtg ccgccttccg
     7081 cgatgcacgg aatgtcgttg ctgcgggggt cgggagtcgg gggtcggggg tcggaggaaa
     7141 agagcgcgtc ctccgactct cgcacgtcct acgccgagtc gttctacccc cgcattcacc
     7201 tcggctggtc ggagcttcgt tccgtcgtgc gaggcactct gcacgtcatc gatgcgccca
     7261 aaccggagat ttacgacctt cgcgccgaca ccggcgagag gaacaacgtt gccgatcgcg
     7321 agcgacgcgc gtttgccgag cttcgcgatg tggcacgcgc tacgtcgggt tcgttcgcgg
     7381 cgccggagcg tgtggacgcc gaggaggcga agaagctggc tgcactcggc tatgtcagcg
     7441 cgggcagcga tgcgggcaac ggtccgctac ccgatccgaa agatgtcatc ggccagcttg
     7501 ccacattgaa gtcggcgcac agcatcgacg atctgcaacg cctgctcgct gcgaatccgc
     7561 gctggtcgga tgttcgcgag caactcggcg gtgagctagg ccgcgtcggt gatcacgcat
     7621 ccgctgcacg cgtgttcgag gaaggcatcc gcatggctcc gcggctggcg ggaacgttcg
     7681 cgctttcagc cggcttcgag ttgctgacga tgcacgatgt cgcaaacgct tcgacccatg
     7741 cgcaactcgc gctctccgcg ggagagccgg gagcgcatct gctgctcgga gagatcgcgc
     7801 tggtgcaacg agacttggcg acggctaccc gcgaggaaac ggcagccgag gagatggcgt
     7861 cgcagaaggc gcacgcgctt tttctggccg cgcggatcgc cgcgatgcag cacgactatc
     7921 cgcgcacgct ccgcatcctc gacgaagtgc agcgcacgcg cgcgaccacg ggcgagtcgc
     7981 tgccggagca ctttcactac gttgcgggtg atgcgttggc gcgaacgaat cggcctgccg
     8041 aggcggagaa cgagatggtt cgggagatcg ccagcgatcc gcacgacctc cgcgcctatg
     8101 tcgatttgtc gcttctgcaa ttcatcggcg gacagcgtga cgacgcggag ataaccctcg
     8161 cgaagctggc tgtgatgcat ccggataccc ctttatctgc tgaggtcgcc cgggaactgg
     8221 caactttcgg cgatgccgca ggaagtcgcg aatgggagca tcgtttacac gatgttcacg
     8281 ggtcgttgac tgatcctgcc ggtcactaac tgacccagaa gttccggtga catgggtcag
     8341 tctttgcgac tttttcgacg cccgtaaatt gaaatcccaa tagaatcatc aacttagctt
     8401 ctggcctctg ccttgcgata gggtgcccgc ctgctagtgg gcaactaacc gattcaagaa
     8461 aggcccggac cgctcaaatt atgaagaagc tgatttcgtt agccgctctc ctcatcacca
     8521 cggttgcttt cgcgaaagat ccagccgcga acagcaagat caagatccag gatcaggatt
     8581 caaagggtgt ccccaccttc gttaccggcg agctgggctc gctcggcgcg ggtgcttcag
     8641 accaggccgt gaaggccttt ctgaaatcgc agaaggatct tctgcagatg gccggcaccg
     8701 aagacttcga cgtcaagagc tcgcagcggg atgcgctcgg acagtcgcac gtgaagttcc
     8761 aggaaaagct gaaaggcctt cctgtcgtcg gcgccgagta catcgttcac tcggattcga
     8821 acgggaacgt tttcgcgatt aacggccgct tcgtggccga ccgcgatctg ccccgcaacc
     8881 cgagcatcga ttccgtcatg gctatccgga cggcagcgac gcagctcggc gcgtgggacg
     8941 cggacatcgt cgacgtac
//
LOCUS       DATLVW010000428         5771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_471267, whole genome shotgun sequence.
ACCESSION   DATLVW010000428 DATLVW010000000
VERSION     DATLVW010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471267"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="VNN25_12955"
     CDS             complement(<1..2001)
                     /locus_tag="VNN25_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXH92484.1"
                     /translation="MPAVVSPPSEPPRGVRRRLTVILLAIAGVLVIAAGLYVLMRARG
                     GAGAPARDIILITIDTTRADSLGYTGNTRVKTPFIDALAGRGIIFTNAHAHNVVTLPS
                     HVNILTGLYAYQHGVHDNSGFVLDPRYLTVATRLRQGGYTTGAFVGAFPLDSRFGLNQ
                     GFDTYDDNYGKGQSTVDFVEQERRATAVLDAATKWWHEKEGQKRFLWVHLYDPHAPYL
                     PPEPFLSAYRYNEYLGEIAYVDDSLSKALGPILAANPDALVVLTADHGESLGEHGEKT
                     HGLFAYESTLKIPLIVARRGIGHRVEPAYVRHVDIVPTILDAAAIAIPKELPGQSLLR
                     AVAQEDSYFESLSASLNRGWAPLTGVIHGGLKYIDLPLAELYDLPRDPQEATNLVTER
                     RRDVETARKLLLSMHPESPATPRAVTPEEVARLRSLGYVTSQAPSRGQFTAADDPKSL
                     TGIDDKMHDAITLFERHDEAKALQLAREVVAERPSMVAGREILAFMLRQNDLVPEAIE
                     QLTIIVRDPGASVDDRVQLALLYCETGRPDQAVALLAPLGATKNPDVLNAYGVALSDQ
                     KKSQQAREQFERVLSFDSNNAPALQNLGILSLREGDVRAALSYLTRALALNPKLPLAL
                     NTLGVAYAQTSDFSRAVDAWNRAVQIDPRQYDALYNIGMVEAR"
     gene            complement(2079..4388)
                     /locus_tag="VNN25_12960"
     CDS             complement(2079..4388)
                     /locus_tag="VNN25_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXH92485.1"
                     /translation="MIEASNDGTAGVSARFPGAIPTLPTWRRGAVPAALRLVQSLIVL
                     LALPLLIGCGRRSIGAPSKTTPVILISIDTLRSDHLPVYGYKGVATPNIDALRADSIL
                     YERAYSHVPLTLPSHVTILTGMLPADSGIRDNIGNRLSESIPILPELLKKNGYATGAA
                     VSAFVLRHETGVARGFDFFEDSTTAMDTSTLVLGRIQRIGGDTLRAVQPWLDKQGAQP
                     FFLFLHLYDPHTPYEPPEPFFSKYPDHYDGEIAYSDSVVGALIDDLKKKDLYDKSLII
                     LLSDHGEGLNEHGEEEHGMFLYREALQVPLLVKLPNSRRGGASVKTPVQLVDVFPTIL
                     ERTETPEPKTGRRTGQSLLTFLDGGPTRSIYSETYYPRFHFGWSDMESLIEGNDHFIR
                     APLPELFDLAADPGEKKNLVQENRRAYVRMKSNIEPMIKEAVAPSGFDPEEAAKLAAL
                     GYVGSTVATQEGKALPDPKTTIGVLHEIRLAFTLFEDKNYPESLRRTNDLLAANGQIV
                     DLWDMKSKILWAMNQPVASIEAAKDGLRRNPGAIALIFDVAQLSLSAGDLDAAQKHAE
                     IAVKIEPGEAHEILSKIWVERHDSARAEQEAKLAMQTVNDPTTELMILAGVEMDRKNY
                     QKAVEYLDGAAARVQKKSPPKLLNLHLTRGDALARLGRGEEAEREFRAEINDFPADAR
                     AYSSLIMLLVSEHRTDEATRVVFDTVKAAPYPHSYVVIAETLKAIGDDRGATFWTSQG
                     LQRFPQDPELRGLPKRMAEMTQKLRSNLQ"
     gene            complement(4385..5368)
                     /locus_tag="VNN25_12965"
     CDS             complement(4385..5368)
                     /locus_tag="VNN25_12965"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92486.1"
                     /translation="MRRLTFLVALILTVAAATIATAQVRGTGRLQGMVTDKATGKPVE
                     GAVVTVGLPSGNTAPIVAKTDAHGHWSALGLVSGQWNIDIVAPGFQTTRGSASVSEMQ
                     QQPMIRTQLAAEVKEEAAAPAVAPTPLIPKEAADAITEGQEMMTQNKYKEAVADFEKA
                     YALVPVDKPETKKVHDQLMQVMAQAYYRAGDLPKAIAMLETITTADPANTGAEVLLAN
                     LYLQNGQLEKGKGLLEKLPASAITDPSAYTNVGILFLNKNNPTDAITYFAKAIEMNPK
                     AGDSYYYRGLAYAQLKKNAEARADFEQVVTLAPDSPEAKDAKAMLAALPKK"
BASE COUNT         1043 a         1743 c         1801 g         1184 t
ORIGIN      
        1 gcgggcctcg accattccga tgttgtagag cgcatcgtac tgacgcggat caatctgtac
       61 ggcgcgattc caggcatcga cagcccggct gaaatcgctc gtctgcgcgt aggccacgcc
      121 cagggtgttg agggccagcg gcagcttcgg attcaacgcc agagcgcggg tgagatagga
      181 cagcgccgcg cgaacatcac cctcgcgcag cgacaggatg ccgaggttct gcagggcggg
      241 agcgttgttg gagtcaaagg agagcacgcg ctcaaactgc tcgcgcgcct gctggctctt
      301 tttctggtcg gaaagagcca ctccgtaagc attcagaaca tcgggattct tcgtcgcccc
      361 cagcggcgcg agcaacgcga cagcctgatc cggccgcccg gtctcgcagt agaggagagc
      421 aagctggacg cggtcgtcga cgctggcgcc cgggtcgcgg acgatgatcg tcaactgctc
      481 gattgcctcc ggaacgagat cattctgacg cagcatgaat gcgaggatct cgcggccggc
      541 gaccatcgat ggccgctccg caacaacctc gcgcgcaagt tgaagagctt tcgcttcgtc
      601 gtgccgctcg aacagcgtaa tcgcatcgtg catcttgtcg tcgatccccg tcagagactt
      661 cggatcgtcg gcagcggtga actgacctcg cgatggcgcc tggctcgtga catatcccag
      721 actgcgaagc cgggccactt cttcaggtgt gaccgccctt ggcgtagcgg gcgactccgg
      781 gtgcatactc agaagaagtt tcctggcggt ctccacatcc cggcgccgct ccgtcacgag
      841 gttcgtcgct tcctgcggat cacgcggaag atcgtagagc tccgcaagcg ggaggtcgat
      901 gtacttcaag ccgccgtgaa tgacgccggt cagcggtgcc cagccgcggt tcaacgatgc
      961 cgacaacgac tcgaagtagc tgtcctcctg cgccaccgcc cgcaataagg attgccccgg
     1021 cagctctttc gggatcgcga tcgcagcggc atcgagaatc gtcggaacga tgtcgacgtg
     1081 gcgcacgtac gctggttcga cgcgatggcc gattcccctg cgggcgacga tcagcgggat
     1141 cttgagcgtc gattcatacg cgaacaggcc gtgagtcttc tcaccatgtt caccgagcga
     1201 ctcgccgtga tcggcggtaa ggacgacgag ggcgtcggga ttggcggcca gaatcggccc
     1261 gagcgccttc gacagggagt catcgacata ggcgatctct ccgaggtact cgttgtatcg
     1321 gtaggcagag aggaaaggtt cgggcggaag gtacggcgca tgcggatcgt atagatggac
     1381 ccacaggaag cgcttctgcc cctccttctc gtgccaccat tttgtcgcgg cgtcgagcac
     1441 cgccgtcgcc cgtcgttcct gctcgacaaa atcgacggtg gactgcccct tcccgtaatt
     1501 gtcgtcgtag gtatcgaacc cctgattcag tccgaacctc gagtcgagcg gaaaggcgcc
     1561 gacgaacgca ccggtcgtat agcccccctg ccgcagtctg gtggcaaccg tcagatacct
     1621 gggatcgagc acgaatccgg agttgtcgtg gacgccgtgc tgataggcgt aaaggccggt
     1681 gagaatgttc acgtgcgacg gcagggtcac gacgttgtgc gcatgcgcgt tcgtgaagat
     1741 gattccgcgt ccggccaggg catcgatgaa cggggtcttg acgcgcgtgt ttcccgtgta
     1801 tccgagcgag tccgcccgcg tagtgtcgat cgtaatgaga atgatgtcgc gagcgggagc
     1861 gccggctcct ccgcgcgccc gcatcagaac gtagagtccc gcggcaatca ccagcactcc
     1921 cgcgatcgcc agcaggatga cggtcaggcg acgcctgacg ccccgaggcg gttccgaagg
     1981 gggcgacacg acagcgggca agacgggttc cttcgcaggt tccgacggtg accggcgacc
     2041 tcgcctcgta ttcttcttca tttaatgaga gccggtcgtc attgcagatt cgaccggagt
     2101 ttctgcgtca tctcggccat gcgtttcggc agcccgcgaa gctcgggatc ctgtggaaag
     2161 cgctgcagcc cctgactcgt ccagaacgtg gcgccccgat cgtcaccgat cgctttgagc
     2221 gtctcggcga tgacgacata cgaatgagga tacggtgccg ccttgacggt atcgaaaacg
     2281 actctcgtcg cctcgtccgt tctatgttcg ctcacgagca gcatgatgag cgacgaatag
     2341 gcgcgcgcat ccgccgggaa gtcgttgatc tcggcgcgga attcgcgctc cgcctcctct
     2401 ccccttccga gccgcgcaag cgcatcgccg cgcgtgaggt ggagattcag aagctttggc
     2461 ggagacttct tctgcacgcg tgcggccgcc ccgtcgagat actcgaccgc cttctggtag
     2521 ttcttccggt ccatctccac gccggcgaga atcatcaact ccgtagtcgg atcgttgacg
     2581 gtttgcattg cgagcttcgc ttcctgttcc gcgcgggcgg aatcatgacg ctccacccag
     2641 atcttggaga ggatctcgtg cgcctcgccg ggctcgatct tcactgcgat ctcggcatgc
     2701 ttctgcgccg catcaagatc gccggccgag agcgaaagtt gcgcaacatc gaagatcagg
     2761 gcgattgcgc cggggttccg gcgcaggcca tctttggcgg cttcgatcga tgcgacgggc
     2821 tgattcatcg cccagaggat tttcgatttc atatcccaga gatcgacgat ctgtccgttg
     2881 gctgccagaa ggtcgttcgt gcgccggagc gactcggggt aattcttgtc ctcgaagaga
     2941 gtgaaggcga ggcgaatctc gtgaagtaca ccgatcgtcg tcttgggatc cggaagcgcc
     3001 ttcccctctt gcgttgccac cgtggacccg acatatccga gcgcggcgag cttcgctgcc
     3061 tcctcgggat caaagccgga aggggcaacg gcctccttga tcatcggttc gatgttgctc
     3121 ttcatcctca cgtaggcgcg gcggttttcc tgcacgaggt tctttttctc gccgggatcg
     3181 gccgccagat cgaacaactc cggtagcggc gcccgaatga aatgatcgtt cccttcgatg
     3241 agcgactcca tgtcgctcca tccgaagtgg aaccgcggat agtacgtttc ggaatagatg
     3301 gaccgcgtag gaccgccgtc gagaaacgtc agcaatgact gtcccgttcg ccgccccgtt
     3361 ttcggttccg gtgtctccgt tcgttcgagg atcgtcggga agacgtcgac gagctgcacc
     3421 ggcgttttca ccgaggctcc gccgcgccgc gaattcggaa gtttcacgag cagaggaacc
     3481 tgcagcgcct cgcgatacag aaacatcccg tgctcttctt cgccatgctc gttgaggcct
     3541 tccccgtgat ccgagaggag aatgatcagc gacttgtcgt acagatcctt cttcttgaga
     3601 tcgtcgatga gagcgccgac gaccgagtcg gagtacgcga tctcgccgtc atagtgatcc
     3661 ggatacttgc tgaaaaacgg ttcgggaggc tcgtacggcg tgtgcggatc gtagaggtga
     3721 agaaagagaa agaacggttg cgcgccctgc ttgtcgagcc acggctgcac tgcgcgaagg
     3781 gtatcccctc cgatgcgctg gattctgccg agaacgagcg tggatgtgtc catcgcggtc
     3841 gtggagtctt cgaagaaatc gaatcctcgc gcgacgccgg tctcgtggcg gagaacaaac
     3901 gccgagactg cagcccccgt ggcatatcca ttcttcttca ggagctccgg aaggatcggt
     3961 atcgactcag aaagacggtt gccgatgttg tcgcggattc cactgtccgc gggaagcatg
     4021 ccggtgagaa tggtgacgtg agagggcagt gtgagcggaa catggctgta cgcgcgctcg
     4081 tagagaatcg agtcggcgcg cagggcgtcg atgttcggcg tggccacacc tttgtacccg
     4141 tagacaggca ggtgatcgga ccgaagcgta tcgatggaga tcagaatgac cggcgtcgtc
     4201 ttcgatggtg cgccgatcga gcggcgtccg cacccgatga gcagcggcaa cgccagcaag
     4261 acaatcagag attgaacgag acggagcgcc gccgggacgg ctcctcgccg ccacgtcggg
     4321 agcgtcggga tagcacccgg aaagcgggct gagaccccgg cggtcccgtc gttgctcgcc
     4381 tcgatcattt ctttggcaac gccgcgagca ttgccttcgc atccttcgct tcgggagagt
     4441 cgggcgcgag cgtaaccacc tgttcgaagt cggctctcgc ttccgcgttc ttcttgagct
     4501 gggcatacgc caacccgcgg tagtagtagc tgtcacctgc tttggggttc atctcgatag
     4561 ccttggcgaa gtaggtgatc gcgtcggtcg ggttgttctt gttcaggaaa aggatgccga
     4621 cgttggtgta ggcgcttgga tcggtgatcg ctgaagccgg cagcttctcg agcagccctt
     4681 ttcctttttc gagctgacca ttctgcaggt agaggttcgc gagcagcact tccgctcccg
     4741 tgttcgcagg gtccgcggtc gtgatggttt cgagcatcgc gatcgccttc ggcaaatcgc
     4801 cggcgcggta gtacgcctgt gccatgacct gcatcagttg atcgtgaacc tttttcgtct
     4861 ccggcttgtc caccggcacg agtgcgtagg ccttctcaaa gtcggccacc gcttccttgt
     4921 atttgttttg cgtcatcatc tcctggccct cggtgatggc atccgctgct tctttcggaa
     4981 tgagcggtgt cggcgctact gccggagccg ccgcttcttc tttcacctcg gccgcgagct
     5041 gcgtccggat catgggctgc tgctgcattt cgctcacgct ggctgatccc cgcgtggtct
     5101 ggaatccggg agcgacgatg tcgatgttcc attggccact gaccaatccg agcgcggacc
     5161 agtgcccatg ggcgtccgtc ttggcaacga tgggggcggt gttccccgag ggaaggccaa
     5221 ccgttaccac ggcgccctca acaggcttgc cggtagcctt gtcggtcacc atcccctgaa
     5281 gccgcccggt tccgcgaacc tgcgccgtag cgatggtagc ggccgcaacc gtcagaatga
     5341 gtgcaaccag gaaagtgagt ctgcgcatga atcctccgtt gatggaaacg tttcgcgggc
     5401 ggtgcctcag aacgcgcgag gccgcgaagc tgttccaatc ttagctcgcc ctcgggtgtt
     5461 ccgcttcttc cttttctctc cgagttcttt ggagtgcttt ctcatcgagc gcattccgcg
     5521 agggccggat gagggacagc caacggctgg cgatcaacct gcacctcgtc cgaactaccc
     5581 gaaaacgagg caaatgttac gagtgtcgtg cttcttgaaa cgcccccctc atccggcttc
     5641 gccaccttct cccccactga aaagcgtggg ggagaaggcg ctcgattgga aggttggaac
     5701 aagagtggaa ggcaagagtg tgagaagggc gggttcttgc ccccaggctt tagcaccgcg
     5761 tttggcaccg c
//
LOCUS       DATLVW010000429        17370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_514851, whole genome shotgun sequence.
ACCESSION   DATLVW010000429 DATLVW010000000
VERSION     DATLVW010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514851"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VNN25_12970"
     CDS             <1..814
                     /locus_tag="VNN25_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HXH92487.1"
                     /translation="GLAALLDRHFGFKVDKKHQRADWAMRPLPPDMLEYAATDTRHLI
                     ALAGRLRDELTALGRWEWAVEEFGRQEQIRFNEKDGDEEPFRRLKGLGALDRRSLAVV
                     RALYDFRDGLAREADRPPFKIFGNDLIFELARVKPASTQDFNSIKGVSGYHRGRFGRD
                     IVRLVKEAMAIPDEELPEKSESKTWLRDREMEARIDRMKKARDKIAKDLKVDASVLAP
                     RHILVSVAEAQPHEPADLDRVPVMRNWQKALLGQALIDALRGGMKAPQSKLF"
     gene            complement(1033..1848)
                     /locus_tag="VNN25_12975"
     CDS             complement(1033..1848)
                     /locus_tag="VNN25_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXH92488.1"
                     /translation="MRFADLHTHTYHSDGTRSPREVIDVALSYGIEIVAISDHDNLAA
                     YFEIKSYADERGVTLIPATELSCAYEGVDIHILAYAFDAHDERIAARLQHFRDARQTR
                     GRRIVEKLRALGYNISSERVEQLAAGGAVGRPHVARALVEGGFVASVSDAFDQLLRPG
                     KPGYVEKDRFAIEEAVALMRATGGLTSIAHPTLYPDHARIVPQLLDAGIDGIEVLHPD
                     VDEASREIYTSVARFRGKMLTGGSDDHGTVKKTETLGTIRVPEAWIGPILDRL"
     gene            complement(2272..5073)
                     /gene="ileS"
                     /locus_tag="VNN25_12980"
     CDS             complement(2272..5073)
                     /gene="ileS"
                     /locus_tag="VNN25_12980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXH92489.1"
                     /translation="MAESSDYKKTLNLPQTSFSMKGNLPQNEPKRLEQWRLIDLYKLI
                     REKSEGRPKSVLHDGPPYANGHIHMGHALNKILKDVVVKSKTMMGYDSPYVPGWDCHG
                     LPIEHAVDKEIGSGKRKEMSPADFRRACRTFAEKFVSIQRQDFIRLGVFGDWFHPYMT
                     MAYQYEADIAYALGRFFRTGMVYKGLKPVHWCTYDQSALAEAEVEYADHTSPSVYVRF
                     RLTDESVKSLDLPIEKPCYAVIWTTTPWTLPANLGIAVKPDFDYEVIEHADENYIIAG
                     ELVPSVITKFGWTEYRVVKVFKGAAFEHLRYRHAFLPREGVFVLGDYVTLDQGTGLVH
                     TAPGHGVDDFKTGQRYGLETYTPVNHRGEFTPDVPLWAGMFVFKANPLIVEHLRERGA
                     LLHAETITHSYPHCWRCKHAVIFRATEQWFISMDDKDLRAKALEQVHSVKWFPAWGEE
                     RMAGMVENRPDWCISRQRTWGVPITVLYCEGCGEPVTSPELFERVKELFLVEGADAWY
                     DRPATDFLPASYGCTKCGGTAFRKELDILDVWFDSGCSHLAVLKERPELTWPSDVYIE
                     GHDQHRGWFQSSLLVGTGLEGAAPFRQVITCGFLINEQGDKMSKSRGNALSPQDVLKQ
                     HGADVIRLWVAMIDYQTDMTIGPQIMGRAAEAYKKIRNTARYLLSNLNGFEPERDAVP
                     HDQLLDVDKWVLARAAEVFDRCRKAYEEYEFHIVYHRVLDLCTVVLSSLYLDISKDTL
                     YCDAPASVSRRSAQTAMYEILRGMVAFLAPLLTFTTEEIYEAMPGAKEQSVHLIDFPT
                     ITAPASDSAAWDRIFRVREAVSKVLEGARAAGTIGQALEADITLHGVTREAIVGTLDL
                     DLAKIFIVSHVDFAAAAIDSTIDIEGVGPIGITMSPARGKKCGRCWQYREEVVEDGGL
                     CARCDEVVSSLEVPEQPTV"
     gene            complement(5129..5377)
                     /locus_tag="VNN25_12985"
     CDS             complement(5129..5377)
                     /locus_tag="VNN25_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92490.1"
                     /translation="MARGFESKAVESNRQDAEDARESRGRGDRIDIHEIERRQKREGL
                     ELSRRRIVTEIEATTSDRRRESLRAALAHLDSELAKLE"
     gene            complement(5630..7000)
                     /locus_tag="VNN25_12990"
     CDS             complement(5630..7000)
                     /locus_tag="VNN25_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXH92491.1"
                     /translation="MKMSRAVVLLLALCTVACATAPPPAPVPKLAVKPAAPPSAARLQ
                     TEEYRQALEAAYGEIVAREGKPVTAPKVDVEAVVSMPIPEHETIRGALQYFTTDLKPS
                     IQESLIRSAKYKNLIDKALDENKLPRGLAYLPVIESAYVPTLTSRAGAHGIWQFMPET
                     AREYGLRVDWWVDERADPERSTRAAATYLRDLYRQFNDWPLALAAYNAGPGRIRRAME
                     STGASTFWELLDAEAVPKETRGYVPTFFATLTIASDPVTYGFRLGSPADLDRQQVEVE
                     GPLSLRYLATIANVDERLLRDLNPALRQGVVPPGKTTLHVPSKAAAAVTARATTLKNE
                     DANIAVCSYTLRDGQSLSRLARSIGTDVDTLLAMNNLRSADDIGGGDSIYLPVRAREL
                     GSLLAHNDAYYAVKKGDTFFSIAKSHHLTVEELLAINELDSDHKLHPGEKLRVTVPHA
                     LSAAGM"
     gene            complement(7849..8415)
                     /locus_tag="VNN25_12995"
                     /pseudo
     CDS             complement(7849..8415)
                     /locus_tag="VNN25_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
     gene            complement(8459..9172)
                     /locus_tag="VNN25_13000"
     CDS             complement(8459..9172)
                     /locus_tag="VNN25_13000"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HXH92492.1"
                     /translation="MNLGIIGSGRAAWAFASTWKRISWPVVGVATRGNETPFGTRRSI
                     EELAATSDILLVAVSDRAIEEVVATIPETKAIIVHPSGALPAMRGGFSLHPLKSLPPV
                     GEASDLEGTLLVFEGAHRDVAQRIATAAGARFAEITAEAKTRYHAAAVFGSNYVAALL
                     DIAEDLIGIDDVREDLALLARSAIDNWLAHNDGQRFTGPAVRGDEAVMNRHAAALAGQ
                     PDLAELYRLLAAHIVAAAK"
     gene            9294..9638
                     /locus_tag="VNN25_13005"
     CDS             9294..9638
                     /locus_tag="VNN25_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92493.1"
                     /translation="MRFALLLLAIATTANANPPSTCGGTDEYAKALCAYQRRAFTEAE
                     AGFRGIVEKNEVDPQTIRAIYFLARTEMKRGRFDEAAPLFVRIYAMDPAFYASWNCDF
                     LLGECRKAVGRD"
     gene            9652..11184
                     /locus_tag="VNN25_13010"
     CDS             9652..11184
                     /locus_tag="VNN25_13010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXH92494.1"
                     /translation="MAQQSNFELRRKLLQLEALYDVGRALNTLRPEGELIEELMHRAV
                     AVLDAMAGFVFSLDEKLNVQQIFTFELEIPTPANAMLAEPPVKQVLASREPMSVTVSK
                     FLGGTGKDLLLAPMISGETIIGMIGVGGKEERGAKSGSFVEDDLRFLASLAAIGGAAI
                     DNARNFHKLSVLRETLEDENRNLKQRMLRDYNDRLMIGDSPKMQRVIDLVARVADSQA
                     SVLIRGESGTGKEMVARLIHGNSARKDGPFVAINCAALPETLLESELFGIERGVATGV
                     DARPGRFELAKGGTIFLDEVGDIPLALQAKLLRVLQEREVEKLGGRRRIPIDVRILSA
                     THRDLEQMIEKTEFRQDLYYRLKVVEIVLPPLRDRKEDIPKLVRFFLDKYGKREGLHD
                     VRITQEALQQLMRYPFPGNVRELENLIEGSLALTTDPVIDPGDLALPESAAGSGGVEL
                     ITDFPSLEDLQRRYVIRVLAHTKGNMSKAARILGVDRKTLYRMREALSQLGAHVESWD
                     IH"
     gene            11395..11553
                     /locus_tag="VNN25_13015"
     CDS             11395..11553
                     /locus_tag="VNN25_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92495.1"
                     /translation="MKSIAETRSNAISVQYALPMLLAINTFALLAIDQLGGIPAWIKS
                     AAALFLSF"
     gene            11579..12022
                     /locus_tag="VNN25_13020"
     CDS             11579..12022
                     /locus_tag="VNN25_13020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH92496.1"
                     /translation="MTSTAERQMELTLPMVADIEIAAARAAGNLARELGMSSEKIDEM
                     THAIIEACINAREHSGCEDNRIYLRFVGTSQGDGSRIDVWITDHGRGFDPDEARSRRP
                     AVGATHKRGRGLQLIEAHMDEVSISTSSGGGTTIHMVKYEKKNHD"
     gene            12015..12365
                     /locus_tag="VNN25_13025"
     CDS             12015..12365
                     /locus_tag="VNN25_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXH92497.1"
                     /translation="MTEELRVAVDRKANGAVLYTKGYINNVGGEEIANRAYELMDDNV
                     RTLLLNLRDTKIVNSIGISILIEIIEKMIDKGGSIAFCCLTPVIHKTFQIMGLANYAK
                     IFDSEDTALKELGL"
     gene            12461..14092
                     /locus_tag="VNN25_13030"
     CDS             12461..14092
                     /locus_tag="VNN25_13030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH92498.1"
                     /translation="MSPLVNQFAEFLQDLQQLERAALSSHGSLIFDAFAKHTPYDVSA
                     VYLRDVRGPSLRLAAKSDACLAPEVLEGEIESASPTLDPEPRHLIPIRTIRDHFGLVA
                     LNGDAASEEDLQMLHAAGAFAGSVLNNHRLAQEMREGDFQLKYRLWELESLYDIGLSI
                     AGTLNIDALADEILFRMISLINARRAALFLRDGDKFRVYRSFGDTRSDFLDDEITDRV
                     VAAGEALTFEDASECIFPGCIATVAVPIKGNNAVIGVLAAGDRETREGDVGAFEANEL
                     RLLSLFANQVAIALENARLHKEALEKQAMQRELDLAATIQRDILPKSIPTVEGIELAA
                     LARPAKQVGGDYHAFFQREGTLTTIVADVSGKSMPAALLVSAFHAAVQLLFAEGRELG
                     DIATELNRHIHSWSAENKFITMIMITIDPEAETIQFVNAGHNPCYVLTANHMEQLRSH
                     GLPIGILGTSKYMTQTRPFAAGSAVVLYSDGINEAENIGGDEWGNEAFEELLVRSAGN
                     TAVGLRDAIAAAVDAFVGEAPQKDDQTLVIARSAS"
     gene            complement(14179..14958)
                     /locus_tag="VNN25_13035"
     CDS             complement(14179..14958)
                     /locus_tag="VNN25_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXH92499.1"
                     /translation="MILSETTDAVTTITLNRPEKLNAFSGTMREDLLAALRTADADPA
                     CRVVVITGAGRAFCAGGDVEFMFGLQTEGDVERFRKLLDAGRDVVTQIAEMAKPVIAS
                     VNGIAAGAGCNLALACDYRIASEAAKFGETFVRIGIHPDWGGTWLLPRMIGTGRALEM
                     MMTGRMVDAAEALAIGIVERVVAADALANETTKLAQTIAQGPPLAIAGIKRALVASRT
                     NTLRAQVELESEHQIEAFRSRDAAEGMAAFFEKRVAKFEGR"
     gene            15064..16152
                     /gene="cax"
                     /locus_tag="VNN25_13040"
     CDS             15064..16152
                     /gene="cax"
                     /locus_tag="VNN25_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HXH92500.1"
                     /translation="MKRVATWLLYATIPVAIALQFMHGSLVWEFVFACAAVLPLAAWI
                     GISTEQLAHRMGSTYGALFNATFGNFAEMVIAIFAIRAGLPNVVRASFTGSIVGNLLF
                     VAGLSMLAGGWKHPTLKFNALAAESQAGQLILAVASFLVPALFFRSVHSAHEPQLIHS
                     VSIGTAIILIVVYILGLVFAFKTHKEQLSAVTAPPEMADEDVWSVRKAIGLLVFASAL
                     MSVVAEGLVHAVGAAGKAWGMNEVFLGFVVLAIVGNAAEHSTAVMLAARNQMDTALNI
                     SMQSSVQIALFVTPMLVFFSYPLGHPLDLIFTPFEMLAVVLGVAIFAYLVMDGETNWY
                     EGIQLLAVYAIIAVALYFLPSGPAPVTP"
     gene            16193..17161
                     /locus_tag="VNN25_13045"
     CDS             16193..17161
                     /locus_tag="VNN25_13045"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH92501.1"
                     /translation="MKAGRPYFFEIFTLANFLLLLLVTWRMTSMPLSTLGVIAPSIAG
                     VFLLQALVGMAIRLALAARKGSARELLVVYRSKGWLIDTLRLAVFAGLWMHTYGWIKL
                     MIPLLHPRLFDQQLWNLDRAIFFGYSPNVFLVTIFSAPPVLRTIDWTYANVFFASINI
                     ASIFFASAPEKRLRIAFMNSNVLLWLIGAWLYVALPSLGPAYRFPEVWLPLAPLLDRT
                     QTFQRLLMTNYDAVVSQLRGVRRPFNILFGVAAFPSLHVAFQMLAFLWMRRLTSWGGI
                     VFGIFAVFIFIGSIVTGWHYLIDGVAGALLAWLCYAAARRVPAIRA"
     gene            17184..>17370
                     /locus_tag="VNN25_13050"
     CDS             17184..>17370
                     /locus_tag="VNN25_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92502.1"
                     /translation="MPADTERQRALGEILARRTAEGAPELYDKIENAAVRCYSCGHRC
                     KIPEGLKGICKVRYNEGG"
BASE COUNT         3141 a         5398 c         5424 g         3407 t
ORIGIN      
        1 tggtctggcc gcgctgctcg accgccattt cggtttcaag gtcgacaaga agcatcaacg
       61 cgctgactgg gcgatgcgtc cgctgccacc cgacatgctc gaatacgcgg ccaccgacac
      121 gcggcatctg atcgcgctgg ctggacggct tcgcgatgaa ctgaccgcgc tgggccggtg
      181 ggagtgggcg gtggaggagt tcggcaggca ggagcagatc cgattcaacg aaaaggatgg
      241 cgacgaggag ccgttccggc gtctcaaggg gctcggcgcc ctcgaccgcc gctcgctggc
      301 cgtcgttcgc gcgctctacg attttcgcga tggtctggcc cgcgaggccg accgtcctcc
      361 gttcaagatc ttcggcaatg acctgatctt cgagctggcc cgcgtcaagc cggcgtcgac
      421 gcaggatttc aacagcatca agggtgtctc cggctatcac cgcggccgct tcgggcgcga
      481 catcgttcgt ctggtgaagg aagcgatggc gattccagac gaggagttgc ccgagaagtc
      541 agagtcgaag acctggctgc gcgaccgcga gatggaagcg cgcatcgatc ggatgaagaa
      601 ggcccgcgac aagattgcca aagacctgaa agtcgacgcc tcggtgctcg cgccccgaca
      661 catcctcgtt tcggtcgcgg aggcccagcc gcacgaaccg gccgatctcg atcgtgtccc
      721 cgtcatgcgc aactggcaga aagccctcct cggccaggct ctcatcgacg cgctgcgagg
      781 cggcatgaag gcgccgcagt cgaagctgtt ctgagcggaa aacgcttcaa atcattggag
      841 catcaggccg gagatccaac catcgaggcg ggacgcctcg atggccgcgg gcgaagacgc
      901 ccgcactcct cagcgctgcg ataaggagtg aggtcgtctc gaccgcgggc acttgggcgt
      961 cctcgcccga gtgcggccgg ggcgcgtgac gtaccgcgtc gtaggttctg ccgcgcagac
     1021 tcctgagaac cgctacagcc ggtcgagaat cggaccgatc cacgcctcgg gcactctaat
     1081 tgtgcccagc gtttcggtct tcttaacggt gccgtgatcg tccgagccgc cggtcagcat
     1141 cttgccgcgg aagcgggcga cgctcgtgta gatctcgcgc gatgcttcgt cgacgtcggg
     1201 gtgaagcacc tcgatgccgt cgatgccagc gtcgaggagt tgcgggacga tgcgcgcgtg
     1261 atcgggatag agcgtcggat gcgcgatcga cgtcaagccg ccggttgcgc gcatcagcgc
     1321 gacggcctcc tcgatcgcga agcggtcctt ttccacgtag ccaggcttgc cggggcggag
     1381 cagttgatcg aaggcgtccg aaacagaggc gacgaaacca ccttcgacga gcgcgcgcgc
     1441 tacgtgtgga cggcctaccg cgccaccggc agcaagctgc tcgacgcgct cggagctgat
     1501 gttgtacccg agggcacgca gcttctcgac gatgcgccgg ccgcgtgtct gtcgcgcgtc
     1561 gcggaaatgc tgcaaacgcg ccgcgatccg ttcgtcatgc gcatcgaacg cgtaggccag
     1621 gatgtggatg tcgacgcctt cgtacgcgca cgacagctcc gtcgccggaa tgagcgtgac
     1681 gccgcgctcg tccgcgtagg acttgatctc aaagtacgcg gcgagattgt cgtgatcgct
     1741 gatggccacg atctcgatgc cgtacgacag cgcgacatcg atgacctcgc gtggcgaacg
     1801 ggtgccgtcg gagtgatagg tgtgcgtgtg aaggtcggcg aagcgcaagc ggggttagcg
     1861 taacaggtgt ggtcgcggtt gcgggtcgcg cggtttcgcc agtcacacgg ttggtcggtt
     1921 tcgcggccgc gcagtgttaa ggatgttgtt cttctcttcg cgcccttcga gaatcgaggg
     1981 acgaagcatt ggtgtcatgg tcccgttatc cggtgtcatc ccttgtgtca tcctgagaca
     2041 gccggagccg ccgtacgtag acgacccctc gcgcagcgtg gagtggcggc ggaggacggc
     2101 gaaggatctc aaattacgat gcacatgcca cacgcgtcgc attcggagat ccttcgccgt
     2161 cctccgccgc cgagcacgca ccgcgcaaac atcgcgattc gagcgggcgg ctgcggcagt
     2221 ctcaggatga cacctaactt tcttcgccgc gaacaccctg gatgaccgca tctaaaccgt
     2281 gggctgctcg ggcacttcga gtgacgacac gacttcgtcg catcgcgcgc acaagccacc
     2341 atcttcgacg acctcttcgc gatactgcca gcagcggcca catttctttc cgcgggcggg
     2401 cgacatcgtg atgccaatcg gtccgacgcc ttcgatgtcg atcgtcgaat cgatcgcagc
     2461 agccgcgaag tcgacgtgcg acacgatgaa gattttggcc agatcgagat cgagcgtgcc
     2521 tacgatcgcc tcacgcgtga cgccgtgaag cgtgatgtca gcctccagcg cctgcccgat
     2581 cgttccggcg gcgcgcgcgc cttcgaggac tttcgacacc gcctcgcgga cgcggaagat
     2641 gcgatcccac gcggcggaat cgcttgcggg cgcggtgatc gtagggaagt cgatgagatg
     2701 caccgactgc tccttcgctc ccggcatcgc ctcatagatc tcttctgtcg tgaacgttag
     2761 cagcggcgcc aggaaggcca ccatgccgcg caggatctcg tacatcgcgg tctgcgccga
     2821 gcgccggctg acagacgccg gtgcatcgca gtagagcgtg tctttcgaga tgtcgaggta
     2881 gagcgatgac agcacgacgg tgcagaggtc gagcacgcgg tgatagacga tgtggaattc
     2941 gtactcttcg taagccttgc gacagcgatc gaacacctcg gcagcccgcg ccagcaccca
     3001 cttatcgaca tcgagaagtt gatcgtgagg aacggcatcg cgctccggct cgaagccatt
     3061 gaggttcgag agcaggtagc gcgcggtgtt tcggattttc ttgtaggcct ctgcggcgcg
     3121 tcccatgatt tgcgggccga tcgtcatgtc ggtctggtaa tcgatcatcg caacccagag
     3181 acggatgaca tccgcgccgt gctgcttgag cacgtcctgc ggcgatagcg cgttgccgcg
     3241 cgacttcgac atcttgtcgc cctgttcgtt gatgagaaag ccgcaggtga tgacttgacg
     3301 gaacggcgcg gcgccttcga ggccggtgcc gacgagcagc gacgactgga accatccgcg
     3361 atgctgatcg tgcccttcga tgtagacgtc gctcggccac gtcaactcgg gacgctcttt
     3421 cagcacggcg agatgcgagc agccggagtc gaaccagacg tcgaggatgt cgagctcctt
     3481 gcggaacgct gtgccgccgc actttgtaca tccgtagctc gcgggcagga aatcggtggc
     3541 cggacggtcg taccaggcat cggcaccttc gactaggaac agctctttca cgcgctcgaa
     3601 aagctcgggc gacgtgaccg gctctccgca accttcacaa taaaggacgg tgatcggaac
     3661 gccccaagtg cgctggcgcg agatgcacca gtcggggcgg ttctcgacca tgcctgccat
     3721 gcgctcttcg ccccaggcag ggaaccactt gacgctgtga acctgttcga gggcctttgc
     3781 gcggaggtct ttgtcgtcca tcgagatgaa ccactgctcg gtggcgcgga agattacggc
     3841 gtgcttgcag cgccagcaat gcgggtacga gtgtgtgatc gtctcggcgt gcaggagcgc
     3901 gccacgctcg cgcaggtgct cgacgatcag cggattagcc ttgaacacaa acatccccgc
     3961 ccacagcggc acatccggcg tgaactcgcc gcgatgattg acgggcgtgt aggtctcgag
     4021 gccgtagcgc tgcccggtct tgaagtcgtc gacgccgtgt ccgggcgccg tgtgcacgag
     4081 gccggtgccc tgatcgagcg tgacgtagtc gccgagcacg aagacacctt cgcgaggcag
     4141 gaacgcgtga cggtagcgaa ggtgctcaaa cgcggcacct ttgaagactt tgacaactcg
     4201 gtactcggtc cagccgaact tcgtgatgac ggacggcacc agctcgccgg cgatgatgta
     4261 gttctcatcg gcgtgctcga tgacttcgta gtcgaagtcg ggcttgacgg cgatgccgag
     4321 gttggcgggg agcgtccagg gggtcgtggt ccagatcacg gcgtagcacg gcttctcgat
     4381 cggaaggtca aggctcttca ctgattcatc agtcaggcga aagcgcacgt aaacagaagg
     4441 agacgtatgg tcggcgtact cgacttcggc ttcggcgagt gccgactgat cgtacgtaca
     4501 ccagtgcacc ggcttgagtc ctttgtagac catgccggtg cggaagaagc ggccgagggc
     4561 ataggcgatg tcggcttcgt actgatacgc catcgtcatg tagggatgga accagtcgcc
     4621 gaagacgccg agacggatga agtcctggcg ctggatggag acgaactttt cggcgaacgt
     4681 gcggcaggcg cgccggaagt cggccggtga catctccttc cgctttccac tgccaatctc
     4741 cttgtcgacg gcgtgctcga tcggcaggcc atggcagtcc cagcccggca cgtaaggcga
     4801 gtcgtagccc atcatcgtct tcgatttgac gacgacgtct ttgaggatct tgttgagcgc
     4861 gtggcccatg tggatgtggc cgttcgcgta gggagggccg tcgtgcagaa ccgacttcgg
     4921 ccgcccttcc gacttctcgc gaatgagctt gtagaggtcg atgagacgcc attgctccag
     4981 gcgcttcggc tcgttctgcg gcaggttccc cttcatcgag aaggaggttt gcggaaggtt
     5041 cagcgttttc ttgtagtcac tgctctcggc catggtgggg ggcgaaacga acggctgagg
     5101 cgcggcattt caatttgccg agactctgct actccagttt cgccagctcg ctatcgaggt
     5161 gcgcgagcgc agcgcgcaac gattcgcggc gacggtcgct cgtggtcgcc tcgatctcgg
     5221 tgacgatgcg tctgcgcgag agctccaatc cttcgcgttt ctggcggcgc tcgatctcat
     5281 gaatatcgat gcgatcgccg cgacctcggg attcccgtgc atcctcggca tcctgccggt
     5341 tggactcaac tgctttgctt tcgaatcctc ttgccataac aaaagaaccg cgtgcaggtt
     5401 acgtgcgcgg gggtcgggaa tcgggggtcg ggggtcggag atgaggcttg ctagagcaga
     5461 ggcaagagat cagagatcag aagagatgcc tctgatctct ggtctcttgt ctcttgtctc
     5521 ttttctccaa atcaccgccg atcctaacat tctgaagacg gctgtcgaaa atgagccctg
     5581 cttctgctcc gacccccgac ccccgacgtc ctccgacccc cgcaaaccct cacatccccg
     5641 ccgcgctcag cgcatgcggt accgtcacgc gcaacttctc tcccggatgg agcttgtgat
     5701 cggagtcgag ctcgttgatc gccagaagct cctcgacggt aaggtgatgg ctcttcgcga
     5761 tcgagaagaa cgtgtcgccc ttcttcacgg cgtagtacgc gtcgttgtgc gcgagcagcg
     5821 atcccagctc gcgcgctcgc actgggaggt agatcgaatc gccgccgccg atatcgtcgg
     5881 cggagcggag attgttcatc gccaggagcg tgtcgacgtc ggtgccgatg ctacgcgcaa
     5941 gccggctcag cgattgcccg tcgcggagcg tgtacgagca gacggcgata ttcgcatcct
     6001 cgttcttcaa cgtcgtcgca cgcgcagtga cggccgcggc tgccttcgac ggaacgtgca
     6061 gtgtcgtctt gccgggaggc accacgccct ggcggagggc agggttcaga tcgcgtagaa
     6121 gccgttcgtc gacgtttgcg atcgtggcga gatagcggag cgagagcgga ccttccacct
     6181 cgacctgctg acgatcgaga tcggcaggtg atccgagccg gaatccgtag gtcaccggat
     6241 cgctggcgat cgtgagggta gcgaagaagg tgggaacgta gccgcgcgtc tccttcggca
     6301 cagcttcggc gtcgagcaac tcccagaacg tcgaagcgcc ggtcgattcc atggcgcggc
     6361 ggatacgtcc cggccctgcg ttgtacgccg caagcgcgag cggccaatcg ttgaactgac
     6421 gatagaggtc ccggaggtag gtggcagcag cgcgggtgga gcgctccgga tcggcgcgct
     6481 cgtccaccca ccagtcgacg cgcaggccgt attcgcgcgc ggtctcgggc atgaactgcc
     6541 agataccgtg cgcgccggcg cgcgaggtga gggtgggcac gtacgcgctt tcgatcaccg
     6601 gcagatacgc cagaccgcgc ggcagcttgt tctcgtcgag cgccttgtcg atgaggttct
     6661 tgtacttcgc cgaacggatg agtgattcct ggatgctcgg cttgagatcg gtcgtgaagt
     6721 attggagcgc gcctcggatc gtttcgtgct ctggaatcgg catcgacacg accgcttcga
     6781 cgtcaacctt cggcgcagtg accggctttc cctcgcgcgc gacgatctcg ccatacgctg
     6841 cctcgagcgc ctgccggtac tcctcggtct gcagtcttgc ggcggacggt ggcgcggctg
     6901 gtttgacggc cagcttcggt accggcgccg ggggaggggc agtagcacaa gcaacggtgc
     6961 agagggccaa cagcagcacg accgctcggc tcatcttcat cgcaacaccc tcccgcccgt
     7021 ttagagatct tcgaatttta gattgaccga cagcgactgc acatcgctcg tttcgccgag
     7081 gtcgatcgac tcttcctgtg actggacgac gcctccgtca ctttcaaaga ataccgcaat
     7141 gcgcaaacgt ttcgacttcg gcagggtgcc cggaggcgtc ggcagagtga tcgttcggtg
     7201 gacgtcgcgc ttcggtttgg gtggatttct gagtgcgtcg agtggcgacg tttctttctt
     7261 cgacggcgcc ttgggtgtcg attgcgtggc gcgcttccgg agcgattcga gctcggccat
     7321 gatgtcgacc gaggagcgca ccttgacgtg tttgacctcc ggtgccgctt tgctgggccg
     7381 ttcgctctgg ccgaaatcga tgtccggttc gggaggggca ggtgggagct cggggatggg
     7441 tggagccgcg gtaacaggcg gcggcggttc ggggagtacg tccgcttctg agagcacgtc
     7501 ggcttcggag agcacgtcag gaatggacat ctcatcgatg cccgcgagct cggctaggat
     7561 ttcgtcagcg gcggagactt cttcaatcgg ctcttcgacc ggaaggaatt gcgtcgcggc
     7621 gggttccgtg ggcgcttccg acggcaagac cgcagcagcg gtggcaccac cgaacgccgg
     7681 aatcggagcg gtgtcgatct tctttccagg ggcttgcgtt ggttccgcga cgcttccttc
     7741 gctgctgccc gagtcttctc gaacgcggaa gctcgcggct tctgctaccg gacgtcgcga
     7801 ggtcgcgcgc gccggctctt cgccggtcat gcgccgccgc aacgttcgca gcgtcagctt
     7861 cgagatcacc ttcaacgtct cgaaaacgcc atctcctcgg gacgccacag cctcgacgaa
     7921 gtcgaacttg cgctccggat cgatgacatc gacgaccgtc tcggccggag cgatgttcgg
     7981 gaggtcgcgc ttattgattt gaagaaccag agggatctcg tcgatggaga ggccgatctc
     8041 gccgaggttt tcgcggaggt tcttgaagct ctcgaggttc gcgtcgagca tcgccacctg
     8101 cgagtcggcg acgaagacga gaccgtcgac gcctttgagg acgagcttgc gtgtcgtgtt
     8161 gtagaagacc tggccgggga tcgtgtagag ctgcaggcgc gtcttgaacc cgccgatgac
     8221 gccgacgtcg atcggcagga ggtcgaagaa cagcgttcgg tcggtttccg tctccagact
     8281 caccatttcg ccgcggctct gcggcgaggt cttggcgtag atgtggtgca ggttggtcgt
     8341 cttcccacag aggcccggac cgtagtaaac gatcttcgcc gccatctgca tcgtcgcgta
     8401 attgaagaag accatccggg cgaggctccg atcgaaaggg gatcgatggt agcacccgct
     8461 actttgcagc cgcaacaatg tgcgcggcga ggaggcggta gagctccgcg aggtccggct
     8521 gtccggccag cgcggcggcg tggcgattca tgaccgcttc gtctccgcgc acggcaggcc
     8581 cggtgaagcg ttgcccgtcg ttatgcgcaa gccagttgtc gattgcggag cgggcaagga
     8641 gggcgagatc ctctcgaacg tcgtcgatgc cgatgagatc ctccgcgatg tcgagaagcg
     8701 ccgctacgta gttcgatccg aacaccgcgg cggcgtggta tcgcgtcttc gcctccgcgg
     8761 tgatctccgc gaaacgggct ccggccgcgg tggcgatccg ttgtgcgacg tcgcggtgcg
     8821 caccttcgaa gacgagcagc gtgccttcga gatccgatgc ctccccgacc ggtggcagcg
     8881 acttgagtgg atgcagcgag aagcctcctc tcatggcagg cagcgcgccg ctgggatgaa
     8941 cgataatggc ctttgtctcc ggaatcgtgg ccacgacctc ttcgattgcg cggtcgctga
     9001 ccgcgacgag gaggatgtcg ctggttgccg ccaactcttc gatcgagcgt cgcgtgccga
     9061 acggcgtctc gttgccgcgc gtagctacgc cgacgaccgg ccaactgatg cgcttccatg
     9121 tcgaggcgaa agcccaggca gcgcgcccgc tgccaatgat gccgaggttc ataattggag
     9181 tgtatttgcg ggtcaggtct aaagtctaaa gtgccgggga cgatcgtcgc tcgagcgata
     9241 aagccaattc ggcactttag actttagacc tgaccccttc tacactcatg tccgtgcgct
     9301 ttgctctctt actcctcgcc atcgccacga ctgcgaacgc gaaccctcct tccacttgcg
     9361 gcggcaccga cgaatacgca aaagccctct gcgcctacca gcgacgggcg ttcaccgagg
     9421 cagaggcggg atttcgcggg attgtcgaga agaacgaagt cgacccgcag accatccggg
     9481 ccatctactt tctggcacgg acggagatga aacgcgggcg cttcgacgaa gcggcaccgt
     9541 tgttcgtaag aatctacgcc atggatcccg cgttctacgc gagctggaac tgcgattttc
     9601 tgctgggtga gtgcaggaaa gcggtgggaa gggattagca gctggcgact gatggctcag
     9661 caatcgaact tcgaactgcg acgcaagctc ctccagctcg aggcgctcta cgacgtcggc
     9721 cgtgcgctga ataccctccg tcccgaagga gagctcatcg aagagttgat gcaccgcgcc
     9781 gtggccgtgc tcgacgcgat ggccggcttc gtcttctccc tcgacgaaaa gctcaacgtt
     9841 cagcagatct tcaccttcga gctcgagatc cctacgccgg cgaacgccat gctcgccgag
     9901 cctcccgtca agcaggttct ggcatcgcgc gagccgatgt ccgtcaccgt ctccaagttc
     9961 ttgggcggta caggcaaaga tctgctgctg gctccgatga tctccggcga aaccatcatc
    10021 ggcatgattg gagtgggtgg gaaagaggag cgtggcgcca agagcggtag cttcgtcgag
    10081 gacgatctcc gcttccttgc ctcgctcgca gccatcggag gcgccgctat cgacaacgcc
    10141 cgcaacttcc acaagctatc ggttctgcgg gagaccctcg aagacgagaa ccgcaacctc
    10201 aagcagcgca tgctgcgcga ctacaacgac cgcctgatga tcggcgactc tccgaagatg
    10261 caacgcgtca tcgatctcgt cgcgcgcgtg gccgattcgc aggccagcgt gctgattcgc
    10321 ggcgagtcgg gcaccggcaa agagatggtg gcgcggctca tccacggcaa ctcggcgcgc
    10381 aaagatggac cgttcgtggc gatcaactgc gccgcgcttc cggagacgtt actcgagtcg
    10441 gagctcttcg ggatcgagcg tggcgtggct accggagtcg atgcgcgtcc gggcaggttc
    10501 gagctggcca aaggcggcac gatctttctc gatgaagtgg gcgacattcc gctggccctg
    10561 caggccaagc tgttgcgcgt gttgcaggag cgcgaggtgg agaagctcgg tggacgccgc
    10621 cgcatcccca tcgacgtccg cattctttcg gcaacccatc gcgatctcga gcagatgatc
    10681 gagaagaccg agttccggca ggacctttac tatcgcctca aagtcgttga gatcgttctt
    10741 ccgccgctcc gcgatcggaa agaagacatt ccgaaactcg tccgtttctt tctcgacaag
    10801 tacggcaagc gcgaggggct gcacgatgtg cggatcacgc aggaagcgct gcagcaactg
    10861 atgcgctacc cgtttcctgg gaacgtccgc gaactggaaa acctgatcga gggctcgctg
    10921 gcgctgacga ccgacccggt gatcgatccg ggcgatctgg cgctccccga gtcggcggcc
    10981 ggctccggcg gcgtggaact gatcacggac tttccgtcat tggaggatct gcaacgccgc
    11041 tacgtgatcc gagtgctggc ccacacgaag ggaaacatgt cgaaagcggc acgtatcctg
    11101 ggggtcgacc ggaagacgct ttaccggatg cgcgaggcat tgtcccaatt aggggcgcat
    11161 gtggaatctt gggacataca ctgataacgc cataagatgc ctatgtttag cgcagcgtca
    11221 cagtttgaaa ccttgctagt gtggcaattt gacccagtgg cgcactccgt cacatcttgt
    11281 tcaaattctc atgtaaactt cgcagcatca gaggcttaga gagagtggag caggcaaacc
    11341 gggcgtggca gtcgcgttgc tcaagggtgg tcccgacaca tgagggggga caccatgaaa
    11401 agcatcgccg aaacccgcag caacgccatc tccgtccagt acgcgcttcc aatgttgctg
    11461 gccatcaaca cgttcgccct gctcgcgatc gaccagctcg gcggcattcc tgcctggatc
    11521 aagtccgcgg ccgcactctt cctctccttt tgaagacgtc cgacaggatc cccgtgaaat
    11581 gacctcaacc gccgagcggc agatggagct gacccttccg atggtcgcgg atatcgagat
    11641 cgctgccgcc cgtgcggctg gaaacctcgc ccgcgagctc ggaatgagct ccgagaaaat
    11701 cgatgagatg actcatgcga tcatcgaggc ctgtatcaac gctcgcgagc attccggttg
    11761 cgaagacaac cgcatctact taagattcgt cggaacctct cagggcgacg gcagcaggat
    11821 cgatgtctgg atcaccgatc acggtcgcgg attcgatcct gacgaggccc gttcgcgccg
    11881 gcccgctgtt ggcgcgactc acaagcgcgg aagagggttg caactgatcg aggcacacat
    11941 ggacgaggtc agcatctcga cctcctcggg tgggggaacc acaatccata tggtcaaata
    12001 cgagaagaag aatcatgact gaggaactca gagtcgcggt cgaccgcaag gcgaacgggg
    12061 cagtgctcta cacgaagggc tacatcaaca acgtcggcgg cgaagagatc gccaaccgtg
    12121 cctacgagtt gatggacgac aacgtgcgca cactcctgct caatcttcgc gatacgaaaa
    12181 tcgtcaactc catcggcatc agcatcctca tcgagatcat cgagaagatg atcgacaaag
    12241 gcggcagcat cgccttctgc tgcctcactc ccgtcattca caagaccttc cagatcatgg
    12301 gccttgcgaa ttacgcgaag atcttcgaca gcgaggatac ggcgctcaag gagctgggct
    12361 tgtgacgtag gtcggactct ccagtccgac ggctctcgca atggagtcgg actggagagt
    12421 ccgacctagg tcccgcaact gctattcttc cgtcacccgt atgtcacccc tcgtcaatca
    12481 gttcgcggag tttctgcagg atctgcaaca actcgagcgc gctgccctct cgtcgcacgg
    12541 gtcgctgatc ttcgacgcct tcgcgaaaca cactccctac gacgtcagcg cggtctacct
    12601 gcgcgatgtg cgcgggccgt ctctgcgtct tgcggcgaag tcggacgcat gtctcgcccc
    12661 cgaagtgctt gagggggaga tcgagtccgc aagcccgacg ctcgatccag agcctcgtca
    12721 tctcatcccc atccgcacga tccgcgatca cttcggcctc gtcgccctca acggcgatgc
    12781 cgcgtcggaa gaagacctgc agatgctgca cgcggccggc gccttcgccg gctccgtgtt
    12841 gaacaaccac cggctcgcgc aggagatgcg cgagggcgac tttcagctca agtaccgtct
    12901 ctgggagctc gagtcgctct atgacatcgg gctctcgatc gccggaacgt tgaatatcga
    12961 tgcgctggca gacgagatcc tcttccggat gatctcgctc atcaacgccc gccgcgccgc
    13021 gctctttctg cgcgatggcg acaagttccg cgtctatcgc tcgttcggcg acacgcgctc
    13081 cgattttctc gatgatgaga tcaccgaccg cgttgtcgcc gccggtgagg ccctgacgtt
    13141 cgaagacgcc tcggaatgca tcttccccgg ctgtatcgcc accgtggcgg tgccgatcaa
    13201 gggcaacaac gccgtgatcg gtgtacttgc cgcgggcgac cgcgagacgc gcgaaggcga
    13261 tgtcggcgca tttgaggcga atgagctacg cctcctctcg cttttcgcaa accaggttgc
    13321 cattgccctc gagaatgcgc ggctgcacaa ggaagcgctc gagaagcagg ccatgcaacg
    13381 agagctcgat ctcgccgcca cgatccagcg cgacattctg ccgaaatcga ttccgaccgt
    13441 cgaagggatc gagctggctg cgctcgcccg tccggccaaa caggtgggcg gcgactacca
    13501 cgcgtttttc cagcgcgagg gaacgctcac cacgatcgtc gccgatgttt ccggcaaatc
    13561 gatgccggct gcgctattgg tctccgcgtt ccatgctgcc gtgcaactcc tttttgccga
    13621 ggggcgggaa ctgggcgata tcgcaacgga gctcaaccgg cacatccata gctggtcggc
    13681 cgaaaacaag ttcatcacca tgatcatgat taccatcgac ccggaggcgg agacgatcca
    13741 attcgtcaac gcgggccaca atccgtgtta cgtactgacc gcgaatcaca tggagcagtt
    13801 gcgctcccac ggactcccca tcggcatcct cggcacgtca aagtacatga cgcagacgcg
    13861 accttttgcg gcaggttccg cggtcgtgct ctacagcgac ggcatcaatg aagcggagaa
    13921 cattggcggc gacgaatggg ggaacgaagc gttcgaggag ctcctcgtgc gctccgccgg
    13981 caacaccgcc gttggcttgc gcgacgcaat cgccgcagcc gtggatgcct tcgtcggcga
    14041 agcgccgcag aaggacgacc agacgctagt gatcgcacgc agcgccagct agcaaaaccc
    14101 tttgcaccac tgagacgcag aggtcgcaga attcagtttc aacactctgc gtcgtcagtg
    14161 tatttgtggt gcattcctct atctcccctc gaacttcgcc acccgcttct caaagaacgc
    14221 cgccatccct tcggccgcgt cgcgcgaccg gaacgcctcg atctgatgct cgctctccag
    14281 ttcaacttgc gcgcgaagcg tgttcgtacg cgaagccacg agcgcccgct tgatccccgc
    14341 gatcgccagc ggaggtccct gcgcgatcgt ctgcgcaagt ttcgtcgtct cgttcgcgag
    14401 cgcgtcggcc gccacaacgc gctcgacaat cccgatcgcc aacgcctcgg ctgcatcgac
    14461 catccgtcct gtcatcatca tttccagcgc tcgtcccgtc ccgatcatgc gcggcagcaa
    14521 ccacgtcccg ccccaatcgg gatgaatgcc gatcctgacg aaggtctcgc cgaactttgc
    14581 cgcctcggaa gcgatccggt aatcgcacgc cagcgccaga ttgcaccccg cgcccgcggc
    14641 gattccgttc accgaggcga tgaccggctt cgccatctcc gcgatctgcg tcacgacatc
    14701 gcgcccggca tcgaggagtt tgcgaaagcg ctcgacatcg ccttccgtct gcagaccgaa
    14761 catgaattcg acgtcgcctc cggcgcagaa tgcccgcccc gcgccggtga tcacgacgac
    14821 gcgacaggcc ggatcggcgt cagccgtgcg caatgccgcg agcaaatcct cgcgcatcgt
    14881 gccgctgaac gcgttcagct tttcgggacg gttcagcgtg atggtggtga cggcgtcggt
    14941 ggtttcgctg agaatcattg tgcgagttta tcgggagtcg ggtgtaggag taggacggtt
    15001 cgcgctccga cccccgaccc cctactcccg actcccgaca cccgagatac agttacaaac
    15061 cacatgaagc gcgtcgccac ctggctcctg tacgccacga tcccggtcgc catcgcattg
    15121 cagttcatgc atggcagcct ggtctgggag ttcgtcttcg cctgcgcggc cgtgctgccg
    15181 ctggccgcgt ggatcggcat cagcaccgag caactcgcac atcggatggg ctcgacgtac
    15241 ggcgccctct tcaacgccac cttcggcaac tttgcggaga tggtcatcgc catcttcgcc
    15301 atccgggcgg gcctgccgaa cgtcgtccgc gcgtcgttca ccggctcgat cgtcggcaac
    15361 ctgctgtttg tggcgggact ctcgatgctg gcgggtggtt ggaagcatcc cacgctgaag
    15421 ttcaacgccc tcgcagccga gtctcaggcg ggacagctca tcctggcggt tgcttcgttc
    15481 ctcgtgccgg cactcttctt ccgatcggtg cattctgcgc atgagccgca gctcattcat
    15541 tcggtatcga tcggtaccgc catcatcctg atcgtcgtct acatcctcgg gctcgtgttc
    15601 gcgttcaaga cgcacaagga gcagctcagc gccgtgactg cgccgccgga aatggctgat
    15661 gaggacgtct ggagcgtccg gaaagccatc ggacttctgg tctttgccag cgcgctcatg
    15721 agtgtggtgg ccgaggggtt ggtgcacgcg gtgggggcgg cgggcaaagc ctgggggatg
    15781 aacgaagtct tcctcggctt cgtcgttctg gccatcgtcg gaaacgcggc cgagcattcg
    15841 acggccgtga tgctggcagc ccgcaatcag atggatactg cgctcaatat ctcgatgcag
    15901 tcgagcgtgc agatcgcgct cttcgtaacg ccgatgcttg tcttcttctc gtatccgctg
    15961 ggccacccgc tcgatctgat cttcacgccg ttcgagatgc tcgccgtcgt cctcggcgtc
    16021 gcgatcttcg cgtatctcgt catggatggc gagacgaact ggtacgaagg catccagcta
    16081 ctggccgtct acgccatcat cgccgtggcg ttgtacttcc tcccgtcggg tcctgcgcct
    16141 gtcactccct gagtcgcgca tgtccggccg tcttccaaca tacgatgacg ccgtgaaagc
    16201 cgggcgcccg tacttcttcg agatcttcac gctcgcgaac ttcttgctgc tgttgctcgt
    16261 gacgtggcgc atgacctcga tgccactctc caccctcggc gtgatcgctc cttcgatcgc
    16321 gggcgtgttc ctgctgcagg cactggtcgg gatggccatc cgcctcgcgc ttgcggcgcg
    16381 taaagggagc gcgcgcgagt tgctggtggt atatcgatcg aaaggttggt tgattgatac
    16441 gcttcgtctg gcggtgttcg cgggactttg gatgcacacc tacggctgga tcaagctgat
    16501 gatcccgctg ctgcacccgc gtctgttcga tcagcagctc tggaatctcg atcgcgccat
    16561 cttcttcggt tactcaccga acgtctttct ggtaacgatc ttttccgcgc cgccggttct
    16621 gcgcacgatc gactggacct atgcgaacgt gttcttcgcc agtatcaaca tcgcctcgat
    16681 cttcttcgcc tcggcgccgg aaaagcggct acgcatcgca ttcatgaact cgaacgtcct
    16741 tctctggctc atcggcgcat ggctctacgt ggcgctgccg tcgctcggtc cggcctatcg
    16801 tttccccgaa gtctggctgc cgctcgcgcc gcttttggac aggacccaga cattccaacg
    16861 tcttctgatg acgaactatg acgcggtggt gtcgcagctc cgaggagtgc ggcgtccgtt
    16921 caatattctc tttggagtgg ccgcgtttcc aagcctgcat gtcgcctttc agatgctggc
    16981 gtttctctgg atgcggcggc tgacgagctg gggcgggatc gtcttcggca tctttgcggt
    17041 cttcatcttc atcggctcaa tcgtgaccgg ctggcactac ctcatcgatg gcgtcgcagg
    17101 cgctctgctg gcgtggttgt gctacgccgc agctcgccgg gttccggcaa tacgcgcctg
    17161 accgcgcggg ttttccctgt tgcatgccgg cagataccga acgacagcgc gcactaggcg
    17221 agatcctcgc gcggcgcacc gccgaaggcg cgccggagct ctacgacaag atcgagaacg
    17281 ccgccgtgcg ctgctactcc tgcggccacc gatgcaagat ccccgagggc ctcaaaggga
    17341 tctgcaaggt ccgctacaac gagggaggtg
//
LOCUS       DATLVW010000430         4444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_352824, whole genome shotgun sequence.
ACCESSION   DATLVW010000430 DATLVW010000000
VERSION     DATLVW010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352824"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VNN25_13055"
     CDS             complement(<1..498)
                     /locus_tag="VNN25_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92503.1"
                     /translation="MPAPWRKTTSLIAFLFFILAPAVLAGPGAFGVAAIPVCQPRGIH
                     LSWSSSSGATSYKVFRGGTAISGVLPASTLAFNDTTSGTAYTVVATDSRGFTFTSNSA
                     TPAAPNSSFCLNGDLEVAEAAYCAVSGPAVHLAWTAGTDSSYIITDANGDLVAVVDGT
                     KSSFEI"
     gene            complement(534..2288)
                     /locus_tag="VNN25_13060"
     CDS             complement(534..2288)
                     /locus_tag="VNN25_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXH92504.1"
                     /translation="MRLLVATAVAVAVAFGFSAIPAMAAPGAFTLTGTASCFFYTPSV
                     SLQWTSSSGATSYELIRDDGQHTAVTKGSLFIFDTNVVVGGPAHTYFVRASDAGPIPT
                     DSNTVSAAPPANQCSPPPDPFTISGNAYCYPGDPTRTMRPAIHVKWSSVLYATSYDLY
                     RNGVAAGSFKGGAGGYSLDDLLNPGGLTFTYYAVAKNAAGTSTSNSLVVTAPADICVT
                     APPVTVLSGSASCGTGTQVPSVTLQWTFVFGASGWQLYRDGVLYASLPVSSSYVDTNV
                     SAGHTYTYSIATNGLSAPLSNPIRVSVCGPGQFSVTPQVFCNNSASAVRLTWTPSLNA
                     DSYTVMRDSVTLASGLTNTSYTDTSVTVDDVYSYRVIAANSSGSTPTASATVTVGAEV
                     CPPPPITLVAEPSCNQIASPPAPLVTLNWSSGATATSYLILRSGVQIAAVGASTTSLR
                     DTGTTPGLAYDYVVRAVGPGGSTDSNKEFVVINPFICDSACIQSCATSVDTTAVVSTA
                     VHFVLQQQPGCGAIVTWNFGDGATLNDPTTLHAYTSPGQYHWSATVSIGTTEMCQSSG
                     TISVTDAPAPPKRRAVRH"
     gene            complement(2368..3690)
                     /locus_tag="VNN25_13065"
     CDS             complement(2368..3690)
                     /locus_tag="VNN25_13065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXH92505.1"
                     /translation="MADVIMPKMGDAMTEGKVIRWYKNAGDAVKKGEPLLEIETDKVN
                     LDLEAEQDGTLGDVAAAAGQMVEVGGVLATILAEGEKAAPKAAAPATAPAKEQDSKPA
                     PDDAPSRRAIDKKDSIKKSTGEYGEAIDMKAPRIDRTAPAANVVAMPQAEGGRRRSSP
                     LARKMARDMGVSLEQIQGSGPGGRIVASDIKNFKPAAAPAQQKPKAPSLPPLQQLETK
                     DIPLSAMRRTIAKRLAESTGPIPHFYLTAEYDVTNLLALREQLNEIEGIKTSVNDFIV
                     RAAALAIRHHPNVNASWGDDAITQHGEVHIGIAVSTPDGLITPVVRNADEKSVSDIAA
                     EVRSLAEKAKNRKLMPAEYQGSTFTLSNLGAWGIEQFTAIINPPNVAILAIGAATAQP
                     VVIDRQVVIRDRMKVTMSCDHRVVDGALGAEYLRTLRAYIEQPLRLVL"
     gene            complement(3683..>4444)
                     /locus_tag="VNN25_13070"
     CDS             complement(3683..>4444)
                     /locus_tag="VNN25_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HXH92506.1"
                     /translation="PIVEMMTMNFAIVAADQILNHAAKVRYFSGGQVDVPVVIRGPQG
                     AGVQLSAQHSQTFESFYAHFPGIKVVAPATPADAKGLLKTSIRGRDPVIFLESAALYG
                     MKGEVPDDPNFTVPFGVSRIAREGSDVTIVSYSRMLQMSLIAAEKLSQDEGIECEVID
                     LRTLRPLDLEPAYASVTKTHRCVVVQEQWKPFGAAAEIAASITETVFDELDAPVARVT
                     GEDVPMPYARNLELLATPHEQHVIAAVKKVMYNHG"
BASE COUNT          823 a         1322 c         1442 g          857 t
ORIGIN      
        1 gatctcgaag ctgctcttcg tgccgtcgac gacagcaacg agatcgccgt tcgcatcggt
       61 gataatgtag gaggagtcgg tgcccgcggt ccaggccagg tgaaccgccg gcccgctcac
      121 cgcgcagtac gccgcctcgg caacctccag atcgccgttc aaacagaagg acgaattcgg
      181 cgccgccggc gtcgcgctgt tggaagtgaa cgtgaagccg cggctatccg tcgccacgac
      241 ggtgtacgca gtcccggacg tggtgtcgtt gaacgcgagg gtcgatgccg gcagcactcc
      301 gctgatcgcc gttccgccgc gaaagacttt gtacgatgtc gcgcctgaag acgatgacca
      361 ggaaagatgg atccctcgcg gctggcagac aggaatggcg gcgactccga aagctcccgg
      421 cccggcgaga acggcaggag cgagaatgaa aaagagaaac gcgatgagcg atgtggtctt
      481 ccgccacggg gcaggcatag gagtggatga attgtaacgc gcccgagcgc agatcagtgg
      541 cgcacggcgc gtcgtttggg tggtgcaggc gcatcggtga ccgagatcgt tccgctgctt
      601 tgacacatct cggttgtacc gatgcttact gtcgcgctcc aatggtactg acccgggctc
      661 gtgtacgcgt gcaacgtagt cggatcgttc aaagttgcgc catcgccgaa gttccaggtg
      721 acgatcgcac cgcagccagg ttgttgctga aggacgaagt ggacggcggt tgacacgacg
      781 gcggtagtgt caacgctcgt tgcgcacgat tggatgcacg cactgtcgca gatgaaagga
      841 ttgatgacta cgaactcctt gttcgagtca gtcgaaccac ccggaccgac cgcccgaaca
      901 acgtagtcat acgccagtcc tggggttgtc ccagtatcgc gaagcgaagt agtgctcgcg
      961 cccaccgcgg caatttgtac gccgctgcgc aggatgagat agctggtcgc cgtcgccccg
     1021 gacgaccagt tcagcgtcac gagcggcgcc ggcggcgagg cgatctggtt acatgatggc
     1081 tcggcaacaa gcgtaattgg aggaggcgga catacctccg ctccgaccgt aaccgtagcc
     1141 gaagcagttg gcgtcgaccc gctactgttc gctgcgatca cacgatacga gtacacatca
     1201 tcaacggtca ccgaggtgtc ggtgtagctc gtattcgtca ggcccgatgc gagcgtcacg
     1261 gaatcgcgca tgacggtata ggaatcggcg ttcaacgacg gggtccaagt caaccgcacg
     1321 gctgaggcgg agttattaca gaagacttgc ggtgtgacac taaattggcc aggcccacat
     1381 acggagactc taatcggatt cgacaggggc gcggaaagcc cattggtggc gatgctgtat
     1441 gtatatgtgt gtccggccga gacatttgta tcgacatagg aggaagacac tggcaaagaa
     1501 gcgtacagga caccatctcg gtaaagctgc cagccactgg caccaaatac aaacgtccat
     1561 tggagcgtca ccgaaggcac ttgagtacct gtgccgcatg aagcacttcc ggacagcacc
     1621 gtcacgggcg gagccgtaac gcagatgtcg gcgggagcgg tcacgaccaa agagttcgag
     1681 gtcgacgtgc ccgcggcatt ctttgctacg gcgtagtacg tgaacgtcaa tccacccgga
     1741 ttcaggaggt catcgagact gtagccgcca gccccaccct tgaatgagcc agccgctacg
     1801 ccgttcctgt agaggtcata gctcgttgcg tacagcacgc ttgaccactt cacatggatc
     1861 gccggccgca tggtccgcgt tgggtcaccg gggtaacagt atgcgtttcc ggagatggtg
     1921 aatggatccg gcggcggaga gcactggttc gctggcggcg ccgcgcttac tgtgttcgag
     1981 tcggtcggga tcggtccggc atcgctggca cgaacaaagt atgtgtgcgc ggggccaccg
     2041 acgactacgt tcgtatcgaa tataaacaac gagcctttcg tcacggcggt gtgctgtccg
     2101 tcgtcgcgaa tcagttcgta ggaggttgcg ccgctcgacg aggtccactg caacgaaacc
     2161 gacggcgtgt aaaagaaaca tgaggctgtg ccggtgagcg tgaacgcacc gggcgccgcc
     2221 atggcaggaa tggcgctgaa cccgaaggcg acagcgacgg cgacagcggt cgcgactaag
     2281 agacgcaaga tcggaccatt cggtcattct atcagtcgcg aatgtccttg tgtttcgatc
     2341 ggagcggcgt ccccgctgca agcaggtcta cagcaccagc cgcagcggct gctcgatgta
     2401 agcgcgcagc gtgcgcaggt actctgcgcc gagggcgccg tcgacgacgc ggtggtcgca
     2461 ggacatcgtc actttcatcc ggtcgcggat cacgacttga cgatcgatca cgaccggctg
     2521 cgcggtggcg gcaccgatcg cgaggatggc cacgttgggc ggattgatga tggcggtgaa
     2581 ctgctcgatt ccccaggcgc cgaggttgga gagggtgaag gtggatcctt gatactcggc
     2641 cggcatcagc ttgcggttct tcgctttctc ggcgagtgaa cggacctctg cggcgatgtc
     2701 ggagactgac ttctcgtccg cattgcgcac caccggcgtg atgaggccat cgggcgtcga
     2761 gacagcgatg ccgatgtgaa cctcgccgtg ctgcgtgatc gcatcatcgc cccacgacgc
     2821 gttcacgttc ggatggtggc ggatggccag tgccgcggcg cggacgatga agtcgttgac
     2881 gctggtcttg atcccttcga tctcgttgag ttgttcgcga agggccagca ggttggtgac
     2941 gtcgtattcg gccgtgaggt agaagtgcgg gatcgggccg gtcgattcgg ccaagcgctt
     3001 ggcgatcgtt cggcgcatcg ccgaaagcgg gatgtccttc gtctcgagct gttgcagcgg
     3061 cggcagcgag ggagctttcg gtttctgttg cgccggagcg gcggcaggct tgaagttctt
     3121 gatatcgctg gcgacgatgc ggccgcccgg tccgctgccc tgaatctgtt cgaggctgac
     3181 gcccatgtcg cgcgccatct tgcgcgccag cggtgacgat cggcggcggc caccctcggc
     3241 ctgcggcatc gcaaccacgt ttgccgcggg cgcggtacgg tcgatgcgcg gcgctttcat
     3301 gtcgatcgct tcgccgtact cgccggtcga tttcttgatc gaatctttct tgtcgatggc
     3361 gcgccgcgag ggggcgtcgt ccggagcggg tttcgaatcc tgctccttcg ccggcgcggt
     3421 tgcaggcgcc gcagctttcg gcgcagcctt ctcgccctcg gccagaatcg tggccagcac
     3481 gccgccgact tccaccatct gcccggccgc ggcggcgaca tcgccgaggg tgccgtcctg
     3541 ctccgcttcc agatcgaggt tgaccttgtc ggtctcgatc tccagcagcg gctcgccttt
     3601 cttcacggca tcgccggcgt tcttgtacca ccggatgacc ttcccctcgg tcatcgcatc
     3661 gcccatcttg ggcatgatca cgtcagccat ggttgtacat caccttcttc accgctgcga
     3721 tcacgtgctg ctcgtgcggc gtggcaagta actcgaggtt tcgcgcgtac ggcatcggga
     3781 catcctcgcc ggtcacgcgt gcaaccggcg cgtccagctc atcgaacacc gtttcggtga
     3841 tgctggccgc gatctcggcg gcggcgccga acggtttcca ctgctcctgc accacgacgc
     3901 agcgatgcgt cttggtcacc gacgcgtacg caggctcgag gtcgagcggt cgcaacgtgc
     3961 gcaggtcgat cacttcgcac tcgatccctt catcctgcga gagcttctcc gcggcgatga
     4021 gcgacatttg cagcatgcgg ctgtacgaga cgatcgtgac gtcgcttccc tcgcgcgcga
     4081 tgcgcgagac gccgaaggga accgtgaaat tgggatcgtc tggcacttcg cccttcatgc
     4141 cgtacagcgc tgcactctcg aggaagatga ccggatcgcg tccgcggatc gatgtcttga
     4201 gcaatccttt tgcgtcagcg ggcgttgccg gcgcgacgac cttgatcccc ggaaaatgcg
     4261 cgtagaacga ttcgaatgtc tgcgagtgct gcgcggaaag ctgaactccg gcgccttgag
     4321 gaccgcggat gacgaccggc acgtcgactt ggccgccgga gaagtagcgg accttcgcgg
     4381 cgtgattgag gatctgatcc gcagcgacga tggcgaagtt catcgtcatc atctcgacga
     4441 tcgg
//
LOCUS       DATLVW010000431         3793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_515136, whole genome shotgun sequence.
ACCESSION   DATLVW010000431 DATLVW010000000
VERSION     DATLVW010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515136"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1218
                     /gene="ugpC"
                     /locus_tag="VNN25_13075"
     CDS             151..1218
                     /gene="ugpC"
                     /locus_tag="VNN25_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXH92507.1"
                     /translation="MSGLSFHNVAKRFGSVSVIENLNLDIHDHELMVLVGPSGCGKST
                     ALRMIAGLEDVSDGTISIGGRVVNDLAPKDRDIAMVFQSYALYPHMTVRENLEFGLRM
                     RKVPADERAKRVADAAEILGMSQLLERKPKDLSGGQRQRVAVGRAIVRQPAVFLFDEP
                     LSNLDAKLRVQTRAEITKLQATLGTTSVYVTHDQVEAMTMGHRITVMRDGKIQQVGTP
                     REVYEKPANAFVAQFIGTPPMNLIPATVNGGALQTSSFTLPLSDRFRGRVAEGRRVIV
                     GIRPEHLHQGSSDHAPIRAGVDVVEPIGHETIVYASTGAERLVAIFDPGATPRAGETI
                     AMSVDAEALHLFDAETQESLG"
     gene            complement(1302..>3793)
                     /locus_tag="VNN25_13080"
     CDS             complement(1302..>3793)
                     /locus_tag="VNN25_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92508.1"
                     /translation="FGSPVAITSRYRVVASSGSVTNTASVTGSQTDPNPANNSSSLQL
                     VVSPVPTCPTAPVLTSPANGQTQASPVIFTWNGSPGAASYIVSISSNNATQTFPTTAG
                     TLTQTLPDGSYTWNVQAVGNSGCPNTTSATFSFTVCNNPGTPVPSVVALSMTGQTYAV
                     TWTPTDGTTQYELQESLDAAFSAPTSTMTGFTSISFTKNVQAATAFFYRVRAFGGCSP
                     VIGAFSATVPVVIVPLSSLGSTGPNIVVPAGSTQPVTFPLQVPGLPGGTTSFVASVDK
                     PWLAVTPTSGVMPPEGLTFSISADPSSLVNGTWTGTVIIIFGSTSVSSRVHAEDTTKT
                     SIPVSISLTTPVTPGTQSSPASSAVVIPSVGHLAGLGSQWQSDVRIANITALSKKVQL
                     TFSQGSATSSAVKQTTLSIDPGATTALDDIVRNWFGVGALGDSSNGVLTVQPLDAGGK
                     PDTSIVKATVASSRTFNASAIGTLGQFIPAVPLPSFISTAVGSSTILALQQIAQSDTF
                     RTNLGIVEATGKPASALVSVFDGAGSRLLDLPLTVAAGQQLQLNSFLADKGITLTNGH
                     IEVQATGGDGKLTAYASVIDSRTTDPLLVSGVPIGGAGATRFVIPGVASLDTAATWRS
                     DVRIFNGGNTPQTATLTLFPTGNPTASVASSVTIQPGEVKALDDIVHATFNLTNAGGA
                     LHVTTAVSVPLIVTARTYDQTSTGTLGQFVQAVTPADAVGTNDRALQLLQMEDSPRYR
                     TNLGIAEVTGKAVTAEVTVILPDSKVAPKVQIPLGAFEYRQFPIISSLGLGNTYNARI
                     SVKVIDGQGKITAYGSVIDQRTQDPTFVPAQ"
BASE COUNT          651 a         1092 c         1359 g          691 t
ORIGIN      
        1 cagtgtgggt tgcgggggta gggtgtcggg agtcgggggt cggagcgaag tcgagcgggc
       61 gcaagtgtgg gcgcgttcgc cgagtccgta tcggctccct ctccccacac cctacccccc
      121 accccccaac acacgctacc ctacgcaacc atgtctggcc tgagctttca taacgtcgcc
      181 aagcggttcg ggagcgtctc cgtcatcgag aatctgaacc tcgacattca cgaccatgag
      241 ttgatggttc tggtggggcc ttccgggtgc gggaagtcga cagcgttgcg gatgatcgcg
      301 gggctggagg atgtcagcga cggcacgatc tcgatcggtg ggcgcgtcgt caatgatctc
      361 gctccgaagg accgcgacat cgccatggtg tttcagagct atgcgctcta cccgcacatg
      421 accgtgcgcg agaacctcga gttcggtctc cgcatgcgca aagtccccgc tgatgagcgc
      481 gcaaaacgcg tggccgatgc cgcagagatc ctgggtatgt cgcagctact cgagcgcaag
      541 ccgaaggatc tttccggcgg gcagcggcag cgcgtcgcgg tcggccgtgc catcgtccgg
      601 cagccggcgg tttttctttt cgacgagccg ctttcgaatc tcgacgccaa gcttcgcgtg
      661 cagacccgcg cggagatcac gaagttgcaa gcaacgcttg gcacgacgtc ggtctacgtc
      721 acgcacgatc aggtcgaagc gatgacgatg ggccatcgca tcaccgtgat gcgtgacgga
      781 aagatccagc aggtcggcac gccgcgcgag gtgtatgaga aaccggcgaa tgcgttcgtg
      841 gcgcagttca tcggaacgcc gccgatgaac ctcattcctg caacggtgaa tggcggcgcg
      901 ctgcaaacgt catcgttcac gctgccactc agcgacaggt tccgcggacg ggtcgccgag
      961 gggcggcggg tgatcgtcgg gatccggccg gagcatctgc atcagggctc ctcggatcac
     1021 gcgccgatcc gcgccggagt cgacgtcgtc gaaccgatcg gacacgagac gatcgtctac
     1081 gccagcaccg gtgcggagcg tctcgtcgcg atcttcgatc cgggagcgac gccgcgcgcg
     1141 ggcgaaacga tcgcgatgtc cgtcgacgcc gaggcgctgc atctgttcga tgcggaaacg
     1201 caggagagcc tgggctgatt ctcacgcgaa ggcgcgaagc cgcgaagaac gaaccgtttg
     1261 agggtcttcg tgccttcgcg tcttcgcgtg agaaacggga actattgcgc gggaacgaac
     1321 gtcgggtcct gcgtcctctg gtcgattact gagccgtatg cggtgatctt gccctggccg
     1381 tcgatgactt tgacgctgat gcgggcgttg taggtgttgc cgaggccgag gctgctgatg
     1441 atcgggaatt ggcggtattc gaaggcgccg agtgggatct gcaccttcgg ggcgactttc
     1501 gagtcgggaa ggatgaccgt cacctccgcg gtcacggcct tgccggtgac ctcggcgatg
     1561 ccgagattgg tgcggtagcg cggcgagtct tccatctgca gcagttgcag cgcgcggtcg
     1621 ttggtaccga ctgcatccgc aggcgtgacc gcttggacga actggcctaa cgtgccggtc
     1681 gatgtctggt cgtacgttcg ggccgtgacg atgagcggca cggagactgc cgtcgtgacg
     1741 tgcagcgcac cgccggcgtt ggtgaggttg aacgtggcgt gcacgatgtc gtcgagcgct
     1801 ttcacttcgc cgggctgaat cgtgacgctg ctggcgacac tcgcggtggg attgccggtc
     1861 gggaagagcg tcagcgtcgc ggtctgcggt gtgttgcctc cgttgaaaat gcgcacgtcg
     1921 ctgcgccatg tggcagccgt gtcgaggctt gccacaccgg ggatgacgaa gcgcgtggca
     1981 ccggcgccgc cgatgggaac gccggagacg agcagcggat cggtggtgcg gctgtcgatg
     2041 acggatgcgt aagcagtgag ctttccgtcg ccgccggtgg cctgcacttc gatgtggccg
     2101 ttggtcagcg tgatgccttt atcagccagg aaggagttga gttggagctg ttggccggca
     2161 gcgacggtca acggaagatc gagcagacgc gagcccgcgc cgtcgaagac gctgacgagc
     2221 gcggaggcgg gctttccggt ggcttcgacg atgccgaggt tcgtgcggaa cgtgtcggac
     2281 tgtgcgatct gctgcagcgc gaggatcgtg ctggagccca ccgcggtgct gatgaaactc
     2341 ggcagcggca cggcaggaat gaactggccg agggtgccga tggccgaggc gttgaaggtg
     2401 cggctcgatg ccaccgtcgc cttcacgata ctcgtgtcgg gcttgccgcc cgcatcgagc
     2461 ggctgcacgg tcagcacgcc gttcgacgag tcaccgagcg cgccgacgcc gaaccagttg
     2521 cggacgatgt cgtcgagcgc ggtggtggcg ccgggatcga tgctgagcgt cgtctgtttg
     2581 acggccgagg atgtggcgct gccctgactg aacgtcagct gcaccttctt cgacagcgcg
     2641 gtgatgttgg cgatgcggac atcgctctgc cactgcgagc cgaggccggc gagatgaccg
     2701 acggagggga tcacgaccgc cgacgatgcc gggctcgatt gcgtaccagg ggtgaccggc
     2761 gtggtgagac tgatcgagac cggaatggaa gtcttcgtcg tgtcctcagc gtgaacccgc
     2821 gagctcacac tcgtcgatcc gaagatgatg atgacggttc cggtccaggt cccgttcacc
     2881 aggctgctcg ggtcggccga gatcgagaac gtcaatccct cgggcggcat gacgccgctt
     2941 gtgggtgtaa cagccagcca cggcttgtcc acgctcgcca caaacgaggt ggtcccgccg
     3001 ggcagcccgg gaacctgaag cgggaacgtg accggctgcg tgctgccggc cggaaccacg
     3061 atgttcgggc cggtagatcc gagcgatgac agcggcacga tgacgacagg aacggtggcg
     3121 gagaaggcgc cgatcaccgg actgcagccg ccaaaggcgc gcacgcggta gaagaatgcg
     3181 gttgcagcct gcacgttctt ggtgaacgag atcgaggtga aaccggtcat cgtcgaggta
     3241 ggagcgctga acgcggcatc gagcgattcc tgcaattcgt actgcgtcgt tccatcggtc
     3301 ggcgtccacg tcacagcgta agtctggccg gtcatcgaaa gagcgacgac gctgggaacc
     3361 ggtgtgccgg ggttgttgca gacggtgaat gagaacgtcg cggaggtcgt attcgggcag
     3421 ccggagttgc cgacggcctg cacgttccat gtgtaagagc cgtccggcag ggtttgcgtc
     3481 agggtccctg ccgtcgtcgg gaacgtctgc gtcgcgttgt tggacgagat cgagacgatg
     3541 tagctcgctg cacccggcga tccgttccac gtgaagatca ctggtgacgc ctgggtttgg
     3601 ccgttggcgg gtgaggtcag gacgggtgcg gtcgggcagg tgggaaccgg cgagactacg
     3661 agctgcagcg agctgctgtt gtttgccggg ttggggtctg tctgggagcc ggtgactgac
     3721 gcggtgttcg tgacgcttcc cgaagaagcg accacgcgat agcggctggt gatggccacg
     3781 ggactgccga agg
//
LOCUS       DATLVW010000432         5399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_187146, whole genome shotgun sequence.
ACCESSION   DATLVW010000432 DATLVW010000000
VERSION     DATLVW010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187146"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1477)
                     /gene="trpB"
                     /locus_tag="VNN25_13085"
     CDS             complement(260..1477)
                     /gene="trpB"
                     /locus_tag="VNN25_13085"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXH92509.1"
                     /translation="MNRSETRPRAASLPDRRGYFGEFGGRYVPETLMAALDEFETAYR
                     NLRRDRAFRDELDDVLRDFVGRPTALTEAKRFAEACGGMRLFLKREDLNHTGAHKINN
                     AIGQALIAKRMGKQRIIAETGAGQHGVATATACALLGLDCVVYMGEVDMARQELNVFR
                     MRLLGAEVVPVNSGSRTLKDAINEAIRDWVTNVRTTHYIIGSVLGPHPYPLVVRDFQS
                     VIGREALSQLKKRTGAIPDVAVACVGGGSNAIGLFYEMLQHDSIRFIGVEAGGRGPAL
                     GQHAARFSGGALGVLHGTRSMVLQDEFGQIAETHSISAGLDYPSIGPEHAYLQQIGRI
                     EYYDCSDDLALEAFHKLSELEGIIPALESSHALGWLLRNGASIGGEKIVVVNLSGRGD
                     KDVPQIAALEAAR"
     gene            complement(1474..2091)
                     /locus_tag="VNN25_13090"
     CDS             complement(1474..2091)
                     /locus_tag="VNN25_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXH92510.1"
                     /translation="MTKIKICGITNEDDALFCAECGADFLGFIFVPGSPRFIEPEKAG
                     VIVARLRKSGGSSKIVGVFRDASPDYIREIKEVAGFDLVQLHGSESDDDIREIGIPAI
                     KALHVAGSLPDTHATPTAAWLLFDTYDERRAGGTGRRFDWSLMTAYERSKPFFLSGGL
                     NPDNVVAAISLVRPDAIDIASGVETRPGVKDHAAVARLFERVRRP"
     gene            complement(2088..2861)
                     /locus_tag="VNN25_13095"
     CDS             complement(2088..2861)
                     /locus_tag="VNN25_13095"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXH92511.1"
                     /translation="MTILDNIVARTRERLGSEPPPDRDAADHAAINRSPFRLTAALRE
                     DRINIIAEIKSASPSAGSIVDNPDVEAIAFYYELGGAAAISVVTEPDFFRGSRDWIED
                     ASLASGLPVLMKDFIIEESQLIRGIAAGASAILLLASLLDAAQIRDFIAVLDEYTCDA
                     LVEVHDEAELARAVGGGARIIGINNRNLRDFSVDLGASERLALQIPPGVLRVAESGIK
                     TRADIDRLRAAEFDAFLVGESLLRQNDRAAAVRQLVTAP"
     gene            complement(3234..4250)
                     /gene="trpD"
                     /locus_tag="VNN25_13100"
     CDS             complement(3234..4250)
                     /gene="trpD"
                     /locus_tag="VNN25_13100"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXH92512.1"
                     /translation="MIDVAIRKVVDGKDLGREEMHEVFGEVMDGRATDVQKSALLIAL
                     RMKGETSEEITGAALAMRERVSPLTVDRERIIDTCGTGGDGRGTFNVSTIAALVAAGA
                     GASVAKHGNRAVSSSCGSADVLAELGVAIDLDAARMSEVLRRTGISFLFAPKLHPAMG
                     AVAGIRRELGVRTIFNVLGPLTNPAFARRQVLGVYSLHLVETLGRVLLALGAEHALVV
                     HSADGLDEISISAATYVCEVRDGDVREFELTPEEIGAGRHSMTEISGGDARENARIAR
                     EVLGGANGARHEIVAANAGAALYVSGAATTIREGVEMARESISSGQAMTKLQELVAVT
                     NELA"
     gene            complement(4247..4819)
                     /locus_tag="VNN25_13105"
     CDS             complement(4247..4819)
                     /locus_tag="VNN25_13105"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXH92513.1"
                     /translation="MITVVDNYDSFTYNLVQQIEKISAFELQVIRNDAFDVDALLDQE
                     PEAIVISPGPGTPSRAGRILDLIRSNESIPLLGICLGHQAIGEAFGGRIVRGRVPVHG
                     KLSDVRHEGRGLFTGCPTPMQTARYHSLVVDRESMPDALRIDAETDDGVIMAIAHRDR
                     PIYGIQFHPESYGTEGGDRLIANFLLEVRQ"
     gene            complement(5055..5306)
                     /locus_tag="VNN25_13110"
     CDS             complement(5055..5306)
                     /locus_tag="VNN25_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92514.1"
                     /translation="MRITIEISDELFKKTRAVAASRGESLREFVSAAIRARLASTITP
                     IATGSGWRSVFGLADPKSVQRVDAVIESAFEQIDPAAWR"
BASE COUNT          971 a         1707 c         1752 g          969 t
ORIGIN      
        1 cccacgcttt ttgtggggga gaaggtgccg aaggcggatg agggggcact tccgattgtc
       61 gggcctgcgc catcagaaga ggtgcttgtt cccggcaggc gaaaggggct tgtagtgtcg
      121 gtctcgatac tattgacgag ccccctcatc cgccttcggc accttctccc ccgctgaaaa
      181 gcgcgggggc gaagggcgct cggttgtaag tgtcgcgcca gcaacgcgcg tgcagggctg
      241 cttctcatcg cgctcccgtt catcgtgcgg cctccagcgc cgcgatctgc ggcacgtcct
      301 tgtcgccgcg tccgctgagg tttacgacca cgatcttctc gccgccgatc gaggcgccgt
      361 tgcgaagcag ccagccgagg gcgtgggagg attcgagggc ggggatgatt ccttcgagct
      421 cgcttagttt gtggaacgcc tcgagggcga ggtcgtcgct gcaatcgtag tattcgatgc
      481 ggccgatctg ctgcaggtag gcgtgctcgg gaccgatgga ggggtaatcg aggccggcgg
      541 agatcgagtg cgtctccgcg atctgtccga attcgtcctg cagcaccatc gaccgcgtgc
      601 cgtgcagaac gccgagggcg ccgccgctga atcgcgctgc gtgctggccg agggctggtc
      661 cgcgaccgcc tgcttccacg ccgatgaagc ggatcgaatc gtgttgcagc atctcgtaga
      721 agaggccgat ggcgttcgat ccgccgccga cacaggctac ggcgacgtcc ggaatcgcgc
      781 cggttcgttt cttcagttga cttaacgcct cgcgcccgat cacggattga aaatcgcgca
      841 cgaccagcgg atagggatgc ggtccgagga ccgagccgat gatgtagtgc gtcgtgcgga
      901 cgttcgttac ccagtcgcgg atcgcttcat tgatggcgtc cttcagcgtg cggctaccgc
      961 tgttgacggg aacgacttcg gcgccgagga ggcgcatgcg gaagacgttc aactcctggc
     1021 gggccatgtc gacctcgccc atgtagacga cgcagtcgag gccgaggagc gcgcaagcgg
     1081 tagcagtggc aacgccgtgc tgtccggctc cggtctcggc gatgatgcgc tgcttgccca
     1141 tgcgcttggc gatgagcgcc tggccgatgg cgttgttgat cttgtgcgcg ccggtgtgat
     1201 tgagatcttc gcgcttcagg aagagccgca tcccgccgca cgcttcggca aatcgcttgg
     1261 cttcggtaag cgcggtagga cgcccgacaa agtcgcgcag gacgtcatcg agctcgtcgc
     1321 ggaaggcacg gtcgcggcga aggtttcgat acgcggtttc gaattcgtcg agcgccgcca
     1381 tcagcgtctc gggaacgtag cggccgccga actcgccaaa gtagccgcgg cggtcaggca
     1441 acgaagccgc gcgcgggcgc gtctcggatc gattcatggt ctcctcacgc gctcgaacag
     1501 acgcgctact gcggcgtgat ctttgacgcc cggcctggtc tcgacaccgc tggcgatgtc
     1561 gatggcgtcc gggcgcacca ggctgatcgc cgcgacgacg ttatcgggat tgagtccgcc
     1621 ggaaagaaag aacggcttgg atcgctcata cgccgtcatg agcgaccagt cgaaacggcg
     1681 ccctgtgcca ccggcgcggc gctcatcgta cgtgtcgaaa agaagccaag ccgcagtggg
     1741 cgtagcgtgc gtgtcaggaa gcgagccggc gacatgcagc gccttgatcg cggggatgcc
     1801 gatctcgcga atgtcgtcgt cgctctcgct gccgtgcagt tgcacgagat cgaagccggc
     1861 gacctctttg atctcgcgga tgtagtcggg agacgcgtca cgaaacacgc cgacgatctt
     1921 cgatgagccg ccgctcttgc gaagccgcgc cacgattacg cctgccttct ccggctcgat
     1981 gaagcgaggc gatccgggaa cgaaaatgaa tccgagaaag tcggcgccgc attcggcgca
     2041 gaagagcgcg tcgtcttcgt tggtgatgcc gcagattttg attttcgtca tggcgccgtc
     2101 acaagttgcc gcaccgccgc ggcgcggtca ttctgccgca acagcgactc gccgacgaga
     2161 aaggcatcga actccgccgc gcgaagccgg tcgatgtcgg cccgcgtttt gatcccactc
     2221 tcggcgacgc gcagaacgcc tggcgggatc tgcagcgcca gccgctcgga cgcgccgagg
     2281 tcgacgctga agtcgcgaag attgcgattg ttgatgccga tgatgcgcgc tcccccaccc
     2341 accgcccgcg ccagttcggc ctcgtcgtga acttcgacga gcgcgtcgca ggtgtactca
     2401 tcgaggacgg cgatgaagtc gcggatctgc gcggcgtcga gcagcgatgc cagaagcaga
     2461 atcgcgctcg cacctgcggc gataccgcga atgagctgcg actcctcgat gatgaagtct
     2521 ttcatcagca cgggaagtcc gctggcgaga gacgcgtctt cgatccaatc gcgggaaccg
     2581 cgaaagaagt ctggctcggt cacgacggag atagcggcgg cgccgcccaa ttcatagtag
     2641 aacgcgatcg cttcgacatc aggattgtcg acaatggagc ccgcagaggg cgacgccgat
     2701 ttgatctcgg cgatgatgtt gatgcggtcc tcacgcagcg ccgcggtcaa ccggaaaggc
     2761 gagcggttga tcgccgcatg atccgcagcg tcgcggtcag gcggcggttc gctgccgagg
     2821 cgctcacggg tgcgcgcaac gatgttgtcg aggatggtca tgctctgcga tacccccgaa
     2881 gtgagagatc cccggctatc actccaagag cactgacgcg aaacgacctc ggccgccgca
     2941 aaacgtggcg tgtcatcctg agcctgccgg aggcgcccgc gctgcttacg aatttcgcgc
     3001 agatcttcct cggcgccgaa ggacggcgaa ggatctcaag atgcgcggcg cctggcatgt
     3061 gcgtcgcggc tgagatcctt cgccgtcctc cgccgccatc catgagtggc gcgaacgccc
     3121 gagtacgaat ggcggctccg gcagtctcag gatgacacat cagctgaagg cgcgtattca
     3181 ccgacacgtc cgccgctcca cggctggaaa tgacagatgg caagtgctcc gattcaagcc
     3241 agttcattcg tcaccgccac caactcctgc aacttcgtca tcgcctgccc ggaactgatc
     3301 gactcgcgcg ccatctcgac gccttcgcga atcgtcgttg ctgcgcccga cacatacaac
     3361 gccgctccgg cattcgccgc gacgatctcg tggcgggctc cgttcgcgcc gccgaggacc
     3421 tcacgcgcga tccgcgcgtt ctcgcgcgca tcgccgccgg agatctccgt catcgaatga
     3481 cgacctgctc caatctcttc cggcgtcagc tcaaattcgc gcacatctcc gtcgcgcact
     3541 tcgcagacat acgtcgccgc ggaaatcgaa atctcatcta gtccgtctgc gctgtgaacg
     3601 accaacgcgt gctccgcgcc gagggccagc aacacgcggc cgagagtctc gacgagatgg
     3661 agcgagtaga cgccgagcac ctgccgtcgc gcgaaggcgg ggttggtcaa cggtccgaga
     3721 acgttgaaga tcgtgcgcac gccgagctcg cggcggatgc ctgccaccgc ccccatcgcc
     3781 ggatgcagtt tcggcgcgaa tagaaacgag attcctgtgc gcctcagtac ttcgctcatg
     3841 cgagcggcgt ctaggtcgat ggccacgccg agctcggcga gaacgtcggc gctgccacac
     3901 gaggacgaga ccgcgcggtt gccgtgcttc gccaccgagg cgccggcgcc ggcggcaacg
     3961 agcgcggcga tcgtcgagac attgaacgtg ccgcgtccgt cgccgccggt gccgcaggta
     4021 tcgatgatcc gttcgcgatc gacggtcaac ggactcacgc gctcccgcat cgccagcgcg
     4081 gcgcccgtaa tctcctcgga cgtctcgccc ttcattcgca gcgcgatcag aagcgcactt
     4141 ttctgcacat cggtggcgcg gccgtccatg acttcgccga agacctcgtg catttcctcg
     4201 cggccgaggt ccttgccgtc gacgactttg cggatggcga cgtcgatcat tgccgcacct
     4261 ccaacaggaa attcgcgatc aatcgatccc ctccctcggt gccatacgat tcgggatgga
     4321 actggatgcc atagatgggc cggtcgcggt gcgcgatggc catgatcact ccgtcgtcgg
     4381 tctcggcgtc gatgcgcaac gcgtccggca tcgattcgcg atcgacgacg agcgagtgat
     4441 agcgcgcggt ctgcatcggc gtcggacagc cggtgaaaag gccgcgtcct tcgtggcgaa
     4501 cgtcgctgag ctttccatgc accggcacgc gcccgcgcac gatgcgccca ccgaatgctt
     4561 cgccgatggc ttggtgcccg aggcagatgc cgagcagcgg gatggactcg ttggagcgga
     4621 tgagatccag gatgcggccc gcgcgcgacg gcgtgcccgg cccgggcgag atcacgattg
     4681 cctccggctc ctgatcgaga agcgcgtcga catcgaaggc gtcgttgcgg atgacctgca
     4741 actcgaacgc agagatcttt tcgatctgct gcaccaggtt gtaggtgaat gaatcgtagt
     4801 tatcgacaac ggtgatcatg gcagctcctg aacgtcggcg ggcggacaga cgggaatcag
     4861 gcgctacgcc aagggcaccg cgaggtcagg gttgggagtg ggctagagcc aaagacacga
     4921 gtcgaccgga cagctcgtcg actcgttgac ctcatgcgcg gggaagttca tggcgggcgg
     4981 aggatagcat gagttgcgct tcgcgcgttg tcggttgtcg gttggttgcg cttcgcgcgt
     5041 tgtcacagcg aggatcaacg ccaggctgcg ggatctatct gctcgaacgc cgattcgata
     5101 accgcgtcga cgcgctgcac agattttgga tcagcgagac cgaaaacgct acgccatccg
     5161 gagccggtcg cgatcggcgt gatcgtggag gcgaggcgtg ccctgatcgc cgcgctgacg
     5221 aactctcgaa gcgattctcc tctagacgca gcaacagccc tagttttctt gaacaactcg
     5281 tcagagattt cgatcgtgat tctcacggtt tcatgctctc ccagacgctc gaggagtccg
     5341 cgcggaatcg acaacgcgcg aagcgcaacc aaccgacaac cgacaacgcg gcgcctgcc
//
LOCUS       DATLVW010000433         5574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_101077, whole genome shotgun sequence.
ACCESSION   DATLVW010000433 DATLVW010000000
VERSION     DATLVW010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101077"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VNN25_13115"
     CDS             <1..2047
                     /locus_tag="VNN25_13115"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92515.1"
                     /translation="EIGLDMQRPYRICGGLQDNNVWCGPSATLDTHGIANSDWFTVGG
                     GDGFYAQWDPSDPSTVYGESQDGNLLRRNLKTHESRSIRPPAAEGERLRFQWNSPLVI
                     SAFDNKTIYYGGNYLFKSTDRGDSWTRLGGDLTTNQDRDTLPIMGRVPDKLTMSRHDG
                     VQAWPEITTISESPLNRDLLYAGTDDGNLQVTRDGGKTWKNVSPKKGIYVSRIVASRF
                     NEGTAYATLDGHRSNDFAVYVFVTSDYGATWKPIRNGLTDDAGTVHVIREHYRNPKLL
                     FLGTERGLYISNDQGGRWTRLKLNLPTVPVDDIAIHPRENDLVLATHGRSIWVLDDLG
                     AIEQMTDAIPSEDLHVFNTRAATEWRVANRSGVTGHKAFFGPNPPNGALITYFLKSKP
                     AEKEKVKITVSDKDGKLVREIDGTGDVGVNRVTWDLRMRNPNEAPREEGAAAGSEAES
                     EQAGGPGGEAGAGGGGGGGGGGFGGNQGSRVEPGEYTVKVAAGKSEQSTKVVVEDDPR
                     IEMTAADRASRRQALTQLSQMAQRGAATRRSVTGLHTALKDYKKTKLPENVQKAVEEF
                     TKKVDDLCLKVGTPVQCGQPAQPLGWAGPPVVYIPPTVTQRITQLMGGIENYSAAPTA
                     WQLDQIKVLQSMLAEVSTAAQTLTKTDLEALNKMMRDANVPYIAVTAGGGGGGGRRQ"
     gene            2102..3400
                     /locus_tag="VNN25_13120"
     CDS             2102..3400
                     /locus_tag="VNN25_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH92516.1"
                     /translation="MPDFRRTLERAFRRRIVVKDTADAVVASGASAQLDEVLGGDATT
                     TVAAGTVAIMLAGVAAFLNLYATQPLLPMFAHVFAASKAAVGMTVSAATIGVALSAPF
                     CGLIAERAGRKRVIVMSTLLLAIPTLMASTATSLTMLIVWRFLQGLVMPGIFGVAIAY
                     IAEEWPPGNVARTMSLYVSATVLGGFLGRVVIGYAATHRIVPSIAPSWRAGFVVIGAC
                     DLLFGLAIARWLPRDRKAVIATAQPSGGVLRHLRNPKLLATYAVGFNVLFSLVAVFTY
                     ITFHLAAPPYLLTPAQLSSLFVVYLAGLIVTPLAGIGIGHFGSRPVLVVAVLGSMIGV
                     SMTLFHSLPLIMAGLVLCSSGVFVCQSASTSYIQMASLPGGRASAAGLYVACYYTGGS
                     VSGVLPGYFWRFGGWTACVVLVLVVQLMTIGVAAIGWRES"
     gene            3397..4059
                     /locus_tag="VNN25_13125"
     CDS             3397..4059
                     /locus_tag="VNN25_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXH92517.1"
                     /translation="MIDVYFIDVNVSRDVIGALAELLTDAERARADRYRFDDDRRRSI
                     VARAATRRILSRMTGAAPRDLEIVEGEHGKPGLAGGEIELNASHSGDLVALSFARATP
                     VGIDVERRRKVHDALALARRYFSEAELAFVEAAEDIGAAFFIVWTAKEAIVKASGEGI
                     AASDLRGFTVPFAAADLQRVTGSWSVATLEPPMPGYHAALATRTGIGPIVQRVIDASS
                     LL"
     gene            4083..4742
                     /locus_tag="VNN25_13130"
     CDS             4083..4742
                     /locus_tag="VNN25_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXH92518.1"
                     /translation="MVRPAEEQRKYQRVTLVRPLVARIGSARVYVTEASLSGLRVAHQ
                     SNIPPEGSICGIVFEWDGIPVTLECRVTRSTLFKAARNSREKSVYHAGLEIVRGGDAS
                     MMTLRDMITGIVARALDEQKANAHGIPPIAAQTFQTGKGTLFLKYELINGTWRHSETK
                     RPEQPMNGFTISAEEDPEHVAMLCETFANADLEGQALIKLMAELSISKAEGIPTRRYF
                     P"
     gene            complement(4819..>5574)
                     /locus_tag="VNN25_13135"
     CDS             complement(4819..>5574)
                     /locus_tag="VNN25_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXH92519.1"
                     /translation="NFYSTFYGASNAEIALVGDFDPKQVTAQLTEIAGSWKSTTPFTR
                     VPRIYHDVAPTNQSLETPDKANAIFLAGVNLQLRDDDPDYPALVLGNYILGGGTLYSR
                     LGNRIRQKEGLSYGVGSNVQASAFDKNGAFTTFAIYAPQNAAKLETAFKEEVDKAVKE
                     GFTPAEIAEAKKGLLESRKLGRAQDAGLSRTLAAELFTNRTLAWDAALEKKISDLTPE
                     QVNAAVKKWIDPSKITIIKAGDFAKSAAVAPKS"
BASE COUNT          983 a         1736 c         1836 g         1019 t
ORIGIN      
        1 cgagatcgga ctcgacatgc agcggccgta tcgcatctgc ggcggattgc aggacaacaa
       61 cgtctggtgc ggaccctcgg ccacgctcga tacgcacggc atcgccaact ccgactggtt
      121 cacagtcggt ggcggcgacg gcttttacgc gcagtgggat ccttcggacc cgtcgacggt
      181 ttacggtgag tcgcaggacg gcaatctgct gcgccgcaat ctcaagacgc acgaatcgcg
      241 ctcgatccgg cctccggcag ccgagggcga acggctgcgg ttccagtgga actcgccgct
      301 ggtgatctcg gcgttcgaca acaagacgat ctactatggc ggcaactacc tcttcaaatc
      361 gaccgaccgc ggcgactcct ggacgcgcct cggcggcgac ctcaccacca atcaggatcg
      421 cgacacgctg ccgatcatgg gacgcgtgcc ggacaagctc acgatgtcgc gtcacgacgg
      481 agtgcaggcg tggcctgaga tcacgacgat cagcgagtcg ccgttgaacc gcgatctgct
      541 ctacgccggc accgacgatg gcaacctcca ggttacgcgc gacggcggca agacgtggaa
      601 gaacgtgtcg ccgaagaagg ggatctacgt cagccgcatc gtcgcctcgc gattcaacga
      661 gggcacggct tacgccacgc tcgacgggca tcgcagcaac gacttcgcgg tctacgtgtt
      721 cgtgacgtcg gactacggcg cgacgtggaa gccgattcgc aacggactca ccgacgacgc
      781 cggtaccgtt cacgtcatcc gcgagcatta ccgcaatccg aagctgctgt ttctcggtac
      841 ggagcgcggc ttgtacattt ccaacgatca aggtggtcgc tggacccgcc tcaaactcaa
      901 tcttccgacg gtgccggtcg acgatatcgc catccatccg cgcgagaacg acctcgtgtt
      961 ggcgactcac ggccgctcga tctgggtcct cgacgacctc ggggcgatcg agcagatgac
     1021 cgacgcaatc ccatcggagg atctgcacgt gttcaacacg agggcggcga cggagtggcg
     1081 cgtcgccaac cgcagtggtg tgaccgggca taaggctttc ttcggaccca atcctccgaa
     1141 tggcgcgctc atcacctact ttctgaagag caagccggcc gaaaaagaaa aggtgaagat
     1201 caccgtcagc gacaaagacg gcaagctcgt tcgcgagatc gacggcaccg gcgacgttgg
     1261 cgtcaatcgc gtgacgtggg atctgcgcat gcggaatccg aacgaagcgc cgcgcgagga
     1321 aggtgctgcc gcaggttccg aggccgagtc cgagcaggct ggcggcccgg gtggcgaggc
     1381 cggagccggt ggcggcggcg gtggcggggg tggcggtttc ggcggaaacc agggaagtcg
     1441 cgtcgagccg ggcgagtaca cggtgaaggt cgcggcaggc aagagcgagc agtcgaccaa
     1501 agtcgtcgtc gaagacgatc cgcggatcga gatgacggcc gccgatcgcg cgtcacgccg
     1561 ccaggcgctg acgcagcttt cgcagatggc gcagcgcggc gcggccactc ggcgatcggt
     1621 caccggcctg cacaccgcac tcaaggacta caagaaaacg aagctgccgg agaacgtgca
     1681 gaaggcggtc gaggagttta cgaagaaggt cgatgatctg tgtctcaagg ttggaactcc
     1741 cgtgcaatgc ggccagcccg cgcaacccct cggatgggcc ggcccgcccg tcgtttacat
     1801 accgccgacg gtgacgcagc gcatcacgca gttgatgggc ggcatcgaga actactcagc
     1861 cgcgccgacc gcgtggcagc tcgatcagat caaggtgctg cagtctatgc tcgccgaggt
     1921 ctcgacggcg gcgcagacac tgacgaagac ggatctcgag gcgctcaaca agatgatgcg
     1981 cgacgcaaac gtgccctaca tcgctgtcac cgcgggcggt ggtggcggcg gcggacggcg
     2041 gcagtaacgt ggagcggcgt cccgccggaa aacggcggga cgctttcgtt tcacttcgtc
     2101 gatgcccgac tttcgccgaa cactggagcg agcgttccgg cgccgcatcg tcgtcaagga
     2161 cacggcggat gcagtcgtgg cgtcgggcgc gagcgcacag ctcgacgaag tgcttggcgg
     2221 cgatgccacg accacagtgg ccgcgggcac cgtcgcgatc atgctcgccg gcgtcgcggc
     2281 gttcctcaat ctctacgcga cgcagccgct gctgccgatg ttcgctcacg tctttgcagc
     2341 atcgaaagcc gcggtaggaa tgacggtaag cgcggccacg atcggtgtgg cgctctctgc
     2401 acccttctgc ggattgatcg ccgagcgcgc aggccgcaag cgggtcattg tcatgtcgac
     2461 gttactgctg gcaatcccga cgctgatggc atcgacagcg acctcgctga cgatgttgat
     2521 cgtctggcga tttctgcagg gcctcgtgat gccggggatt ttcggcgtgg ccatcgccta
     2581 catcgccgag gagtggccgc cggggaacgt ggcgcggacg atgtcgctct acgtcagcgc
     2641 cactgtcctg ggcgggttcc tcgggcgcgt cgtcatcggc tacgctgcga cgcaccgtat
     2701 cgttccgtcg atcgccccga gctggcgcgc cggcttcgtc gtcatcggcg cgtgcgatct
     2761 tctgttcgga ctcgccatcg cgcgctggct gccgcgtgac cgcaaagcag tgatcgcgac
     2821 ggcgcagcca agtggcggcg ttctgcgcca tctgcgcaat cccaagctgc tggcgacgta
     2881 cgcggtcggc ttcaacgtgc tcttctcgct cgtggccgtc ttcacctaca tcacgttcca
     2941 tctcgccgcg ccgccgtatt tgctgactcc ggcccaactc agctcgctct tcgtcgtcta
     3001 cctcgctgga ctgatcgtca cgccgctcgc ggggatcggc atcggacact tcggctcgcg
     3061 gcccgtgctc gtcgtcgcag tcctcggctc gatgatcggc gtgtcgatga cgctctttca
     3121 ctcgctgccg ctgatcatgg ctggtcttgt gctctgttcg tccggcgtgt tcgtttgcca
     3181 atcggcgtcg acgagctata tccagatggc ctcgctgccg ggaggacggg catcggcggc
     3241 cggactctat gttgcgtgct actacaccgg cggcagcgtc agcggcgtgc tgccgggata
     3301 cttctggcgt ttcggcggat ggaccgcgtg cgtcgtgctg gtgcttgtcg tgcagctcat
     3361 gacgatcggc gttgccgcga tcggatggcg tgagtcgtga tcgatgtcta cttcatcgac
     3421 gtgaatgttt cgcgcgacgt catcggcgcg ctggcggaat tgctcacgga tgcggaacgg
     3481 gcgcgtgcgg accgctatcg attcgacgac gaccgccgcc gttcgatcgt cgctcgcgca
     3541 gccacacgac ggattctttc gcgaatgacc ggcgcggctc cgcgtgacct ggagatcgtc
     3601 gaaggggagc atggaaagcc gggactggcc ggcggcgaga tcgagctcaa cgccagccac
     3661 tccggcgatc tagtcgccct ctcgttcgcg cgagcgacgc cggtcggcat cgacgttgaa
     3721 cggcggcgta aggtgcatga tgcgctggcg ctggcgcggc gttacttctc ggaggcggag
     3781 ctcgcattcg tggaagccgc cgaggacatc ggcgcggcgt tcttcattgt ctggacggcg
     3841 aaggaagcga tcgtcaaggc gagcggcgag gggatcgccg cgagcgacct tcgcggattc
     3901 acggttccgt tcgctgcggc ggatctgcag cgcgtcacgg ggagttggag cgtggcgacg
     3961 ctcgaaccgc cgatgcctgg ctatcacgcc gccctcgcca cgcgaacggg tatcgggcca
     4021 attgttcagc gcgtcatcga tgcgtcgtcg cttctgtaac ggtttgtttt acacttgatg
     4081 cggtggttcg accggccgaa gagcagagaa agtatcagcg tgtgacgctg gtccgtccgt
     4141 tggtcgcgcg gattggctcg gcgcgcgttt acgtcaccga ggcatcgctg agcgggcttc
     4201 gggtggcgca tcagagcaac attccgcccg agggatcgat ctgcggcatc gttttcgagt
     4261 gggatgggat tccggtcacg ttggagtgcc gcgtcacgcg cagcacgctc ttcaaggcgg
     4321 cgcgaaactc ccgcgaaaag agcgtttatc acgccggact ggagatcgtc cgcggcggcg
     4381 acgcctcgat gatgacgctg cgcgacatga ttactggaat cgtcgcccgc gcgctcgacg
     4441 agcagaaagc gaacgcccac ggtattcctc cgatcgccgc ccagaccttt caaacgggga
     4501 agggaacgct attcctgaaa tacgagctga tcaacggcac atggcgtcac agcgagacga
     4561 aacggccgga gcagccgatg aacggattca cgatctccgc cgaggaggat ccggaacacg
     4621 tggcgatgct gtgcgaaacg ttcgcgaacg ccgacctcga aggccaggcg ctgatcaagc
     4681 tgatggcgga gctcagcatc agcaaggccg aggggatccc gacgcggcga tactttccgt
     4741 aaaggctttc acgcggagac gcggagggcg cggagaacct ctccgcatct ccgcgcctcc
     4801 gcgtgacaag gttttgaact acgacttcgg ggcaacagcg gccgatttcg cgaagtcacc
     4861 cgccttgatg atcgtgatct tcgacggatc gatccacttc ttcaccgccg cgttgacctg
     4921 ctccggtgtc agatcggaga tcttcttttc cagcgctgca tcccaggcca gcgtccgatt
     4981 cgtgaagagc tccgcggcca gggtccgtga gagtccggca tcctgcgcgc ggcccagctt
     5041 gcggctttcg agcaggccct tcttcgcttc cgcgatctcg gccggcgtga agccttcttt
     5101 cacagccttg tccacctctt ccttgaacgc cgtctccagc ttcgcggcgt tctgcggtgc
     5161 gtagatggcg aacgtggtga aggcgccgtt cttgtcgaac gcgctggcct gaacattcga
     5221 gccgacgccg tagctcaaac cttctttctg acggatgcgg ttaccgagcc gcgagtagag
     5281 cgtgccgccg ccgaggatgt agttgccgag gacgagggcg gggtaatcgg gatcatcgtc
     5341 gcgaagctgg agattgactc cggccaggaa gatggcgttg gccttgtccg gcgtctccag
     5401 cgactgattc gtcggcgcaa cgtcgtggta aatacggggc acgcgagtga agggcgtcgt
     5461 gctcttccac gatcctgcga tctcggtcag ttgcgcagtg acctgcttcg gatcaaagtc
     5521 gccgaccagc gcgatctcgg cattcgaagc tccgtagaac gtcgagtaga aatt
//
LOCUS       DATLVW010000434         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_471806, whole genome shotgun sequence.
ACCESSION   DATLVW010000434 DATLVW010000000
VERSION     DATLVW010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471806"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNN25_13140"
     CDS             <1..518
                     /locus_tag="VNN25_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92520.1"
                     /translation="IIVGLMAIPYIDTNPKGNGYYSFRERKWEIGVFLYGFLVLWSFL
                     IITGTFLRGPNWNFFGPYEFWDPNKLVPLVNVDLSELMWVKVLGTSLPKLWIIREIFG
                     LALIGAYLGTPPVMAVGPFRRFYLKMGAARYYVGAYLFLIMMSLPIKMVLRWLFNLKY
                     ILHIQEIFFNV"
     gene            535..>3001
                     /locus_tag="VNN25_13145"
     CDS             535..>3001
                     /locus_tag="VNN25_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXH92521.1"
                     /translation="MKPQEPIPHNYSIRRLNMIFALSSLVLLAVTGLMVGYDYIRGWK
                     WFQLEFMRMQQERISQEITVAQAEENKQQLADLDAQDRKNEIELARHRDQYLSAQKEL
                     DAIEGDHYRADQDYRFAKANLDAQRYITEASVVQHLADAPKQQAEYERQTKHLADLQL
                     ALQESTRKRDAAKANVDQWLKKIKDAEDKKKELTASADLLTKQLGTVDMKPFSTNWIL
                     SQPMLDFVNPVIKIDQVVLNDLSIDMNYMNVPRVDRCQTCHRAIDTPGWESKTEAARL
                     SKELQQKLDAYQIPQEKRKETEERIAQLKRIQDAPNDILNPWRTHPKLDLYVGSASLH
                     PLLDYGCTVCHRGQDRATEFGRAGHTPASPRMEHRWAERTMLTAGFFPLPRDFAQRHW
                     GYEENEFIETPMYPRHYYEAGCIKCHSGQMAVDRGEDITKAEQTIEIYGCYACHKIAN
                     WRFSDLRKPGPDLGGIAEKTTPEWAFRWISEPHNFRSTTRMPSFFYQRNMIDPNVVPP
                     QERAHNIKLQDAEIHAIVSFLFDKSTHRPWSQPGAGDAARGKQLVGSVGCMGCHIDTE
                     QVKDEKSGQIRLAKREDFPLERNYGFNLTGVGTKANPAWIYNWVKNPKGYYADAPMPS
                     LRLTDQEAADVTAYLVTMQKPAFMTTPIRPPAAQAVHDLAKGYLINTLSDVEAESKLR
                     SMSQHDQLVYLGQRSIEKYGCYSCHNIKGFEGLKPIGTELTVEGSKALHLFDFGFMND
                     EQWKNEDGSTEHVIHTVPSWVYNKLRNPRIYDDRRTKVYNDKLKMPNFHLTPEEARRI
                     AMVVVGLTKEKVAENRMAGRDART"
BASE COUNT          672 a          942 c          860 g          527 t
ORIGIN      
        1 tcatcatcgt cggactgatg gccatcccgt acatcgatac caatccgaag ggcaacggct
       61 actactcgtt tcgtgaaaga aagtgggaga tcggcgtctt tctctacggc ttcctcgtgc
      121 tctggtcatt cctgatcatt accggcacct tcctgcgtgg cccgaactgg aacttcttcg
      181 gaccttacga attctgggat ccgaacaagc tcgtgccact cgtcaacgtc gatctctccg
      241 agttgatgtg ggtcaaagtc ctcggcacgt cgctgccaaa gctctggatc atccgcgaga
      301 tcttcggact cgccctcatc ggcgcttacc tcggcacgcc gccggtcatg gccgtcggtc
      361 cgttccgccg cttctatctg aagatgggcg cggcgcggta ctacgtcggc gcgtacctct
      421 tcctgatcat gatgtcgctg ccgatcaaga tggtgctgcg ctggcttttc aacctcaaat
      481 acatccttca catccaggag attttcttca acgtctgagg acgacaccta accgatgaag
      541 ccgcaagagc cgattccgca caactactcg attcgcaggc tgaatatgat tttcgccctg
      601 tcgagtctcg ttctgcttgc ggtgacgggt ctcatggtcg gatacgacta catccgaggt
      661 tggaagtggt ttcagctcga gttcatgcgc atgcagcagg agcgcatctc ccaggagatc
      721 acggtcgcgc aggctgaaga gaacaagcag cagctcgccg atctcgacgc gcaggaccgc
      781 aagaacgaga tcgagctggc acggcaccgc gatcagtatc tttccgcgca gaaagagctc
      841 gacgcaatcg agggcgatca ctaccgcgcc gatcaggact accgcttcgc caaggccaac
      901 ctcgatgcgc agcgctacat caccgaagcc tcggtcgtgc aacacctcgc cgacgcgccg
      961 aagcaacaag cggagtacga gcggcagacg aagcatctcg ccgatctgca gcttgcgttg
     1021 caggaatcga cgcgcaagcg cgatgcggca aaagcgaatg tcgatcagtg gttgaagaag
     1081 atcaaagatg ccgaggacaa gaagaaggag ttgacggcgt cggccgatct gctcaccaaa
     1141 cagctcggca ccgtcgacat gaagccgttc agcacgaact ggatcctcag ccagccgatg
     1201 ctcgacttcg tcaatcccgt gatcaagatc gatcaggtgg ttctgaacga tctgtcgatc
     1261 gacatgaact acatgaacgt cccgcgcgtc gaccgctgcc agacctgcca tcgcgccatc
     1321 gatacgccgg gctgggagtc gaagaccgag gcggcaaggc tgtcgaagga gttgcagcag
     1381 aaactcgacg cctaccagat cccgcaggag aagcggaaag agaccgaaga gcgcatcgcg
     1441 cagctgaaac gaattcagga tgcccccaac gacatcctca atccatggcg cacgcacccg
     1501 aagctcgatc tctacgttgg atcggcgtcg cttcacccgc tgctggatta cggctgcacg
     1561 gtttgccatc gcggccagga tcgcgccacc gagttcggcc gcgccggcca cacgccggca
     1621 agtccacgca tggagcatcg ctgggccgag cgcacgatgc tgacggccgg cttcttcccg
     1681 ctgccgcgcg atttcgcgca acggcactgg ggctacgaag agaacgagtt catcgagacg
     1741 ccgatgtatc cgcgtcacta ctacgaggcc ggctgcatca agtgccactc gggccagatg
     1801 gccgtcgatc gcggcgagga catcacgaag gcggagcaaa cgatcgagat ctacggctgc
     1861 tacgcctgcc acaagatcgc caactggcgc ttttccgatc tccgcaagcc tggtcccgac
     1921 ctcggcggga tcgcagagaa gacgacaccg gagtgggcct tccgctggat cagcgagccg
     1981 cacaacttcc gctccacgac gcgcatgccg tcgttcttct accagcggaa catgatcgat
     2041 ccgaacgttg tgcctccgca ggaacgggcg cacaacatca agctccagga tgccgagatc
     2101 cacgccatcg tcagcttcct gttcgataag tcgacgcacc ggccgtggtc gcagccgggc
     2161 gccggcgacg ccgcccgcgg caagcaactc gtaggcagcg tcggctgcat gggctgtcac
     2221 atcgacacgg agcaggtcaa agacgagaag agcggccaga ttcggctcgc gaagcgcgaa
     2281 gacttcccgt tggagcgcaa ctatggtttc aacctgaccg gggtcggaac gaaggcgaat
     2341 ccagcctgga tctacaactg ggtgaagaac ccgaagggct actacgccga cgccccgatg
     2401 ccgtcgctgc ggctcaccga tcaggaagca gcggacgtca cggcgtatct cgtgacgatg
     2461 cagaagccgg cgttcatgac gactcccatc cgcccgccgg ctgcccaggc ggttcacgat
     2521 ctcgccaagg gctacctcat caacacactg agcgacgttg aggcggaatc aaagctgcgc
     2581 tcgatgtccc agcacgatca gctcgtctac ctcgggcagc gatcgattga aaagtacggt
     2641 tgctactcct gccacaacat caaaggattc gaaggcctca aaccgatcgg taccgagctg
     2701 acggtagagg gctcgaaagc gctgcacctc ttcgatttcg gcttcatgaa cgacgagcag
     2761 tggaagaacg aggatggctc caccgagcac gtcatccaca cggtgccgtc gtgggtctac
     2821 aacaagcttc gcaatccgcg tatctacgac gatcgccgca cgaaggtcta caacgacaag
     2881 ctgaagatgc cgaacttcca cttgacgccg gaggaagcgc gccgcatcgc catggtcgtc
     2941 gtcggattga cgaaggaaaa agtcgccgaa aaccgaatgg cgggacgcga cgctcgcaca
     3001 c
//
LOCUS       DATLVW010000435         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_227965, whole genome shotgun sequence.
ACCESSION   DATLVW010000435 DATLVW010000000
VERSION     DATLVW010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227965"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..425)
                     /locus_tag="VNN25_13150"
     CDS             complement(12..425)
                     /locus_tag="VNN25_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXH92522.1"
                     /translation="MALFSTKEPTPQPRGLDAQPVAGGTFIGSNLTIDGTVTGSEPVL
                     IEGTIRGKINLSGDLRIGSKARVEATVHAKNVTVEGKLTGDLSADDKVELVASATVDG
                     NIKAPKIIVAEGARFRGSVDMGSAKPATAQGEPGK"
     gene            720..2111
                     /locus_tag="VNN25_13155"
     CDS             720..2111
                     /locus_tag="VNN25_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH92523.1"
                     /translation="MRRLAVFLTIAALAACKGSGNKNEQYRTEKVDRGTITMTVTATG
                     TLSAVTTVQIGSQVSGVIAALHADFNSRVTKGQLLAELDPTPFQQTVEQRQADLTKSK
                     VEAANSRISYDRAQRLVKSGLSPEADLDAARAAYEEANAQVQQSTAALNQSLTNLKYT
                     KIISPIDGVVVDRQYDIGQTVAASFSAPTLFSIAQDMTKMQVQADVDQSDIGRIAVGQ
                     TARFTVDSYPDQEFRGRIAQIRLNATVSQNVVTYPVIIEVPNPDEKLRPKMTANVTID
                     VASVQDVLRIPNSALRFKPDTAALPAKPATTTSAATDTTQTTQTQPTQTQTAATTGGP
                     TSDQRAERRRQRMEAGGATSTMSPEERAARWGNRARGMQGAAGALGGAPPMRKRPQTV
                     YMMDADGKLKPVEIRTGITDGHYTQVVSGDLKVGDMIIVGLATSKVETNAPPGSAGPM
                     GGGARPGGGRGGR"
     gene            2159..>2280
                     /locus_tag="VNN25_13160"
     CDS             2159..>2280
                     /locus_tag="VNN25_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH92524.1"
                     /translation="MGEVEVHALAGLDLEVDEGEFLAVMGPSGSGKSTLMNILG"
BASE COUNT          468 a          740 c          688 g          384 t
ORIGIN      
        1 ctcgaaaccc gctacttccc cggctcaccc tgagccgtcg ccggcttcgc cgaccccatg
       61 tccaccgagc cgcggaaacg tgctccctcg gcgacgatga tcttcggcgc tttgatgttc
      121 ccgtcgaccg tggcgctggc aaccagctcc actttgtcgt ccgccgatag gtcaccggtt
      181 aactttcctt ctaccgtcac gttttttgcg tgcacggtag cttcgacacg cgcttttgag
      241 ccgatccgaa gatcgccgct gaggttgatt ttccctcgta tggttccttc gatgagcacg
      301 ggctccgagc cggttaccgt gccatcgatc gtgaggtttg agccaatgaa cgtaccgccg
      361 gcgacgggtt gcgcatcgag gccccgaggt tgaggagtgg gttctttcgt tgagaaaagc
      421 gccatggggg cggaagcata gcagagcggt gaagcagaga tcagtggagc agagatcagt
      481 ggagcagaga tcagagatca ggagggatca gggatcgggc agcgcaaccc ggatctctgc
      541 ttcccgatct ctgatctcta ttccctgatc tctgcttctc cctggtctct gactcacgtg
      601 acgaatcggt agcggcccgt aaccgagccg ttgcaggcgg gatcactgca ttacactgcg
      661 ccccgctcat tcaacaccca gctcaacggg tcgtacggag cgcggaggga gttcccagcg
      721 tgagacgttt agccgtattt ctgacgattg ctgctctggc agcgtgcaaa ggcagtggca
      781 acaaaaacga acaataccga accgagaaag tcgatcgcgg cacgattacg atgacggtca
      841 ccgcgaccgg cacgctgtcc gccgtcacca ccgttcagat cggcagccag gtttccggcg
      901 tgatcgctgc acttcacgcc gatttcaaca gccgtgtgac gaaaggccag ctcctcgccg
      961 agctcgaccc gacgccgttc cagcagacgg tcgagcagcg gcaagccgac ctgaccaaat
     1021 caaaggtcga ggcggccaac tccaggatca gctatgaccg cgcgcagcgg ctggtgaaat
     1081 caggactgtc gcccgaggcc gatctcgacg ccgcgcgagc ggcctacgaa gaggccaatg
     1141 ctcaggtcca gcaatcgacc gcggccctca accagtcgct gaccaatctc aagtacacga
     1201 agatcatctc gcccatcgac ggcgtcgtcg tcgaccggca gtacgacatc ggccagaccg
     1261 tcgccgcctc gttctccgcg ccgacgctct tctccatcgc acaggacatg acgaagatgc
     1321 aggtgcaggc cgacgtcgat cagtccgaca tcggacgcat cgccgtgggc cagaccgccc
     1381 gcttcacggt cgactcctat cccgatcagg agttccgcgg acgaatcgcg cagatccggc
     1441 tcaatgcgac ggtcagtcag aacgtcgtca cctatccggt catcatcgaa gtgccgaatc
     1501 ccgacgaaaa gctgcgcccg aagatgactg cgaacgtcac catcgacgtc gccagcgtgc
     1561 aggacgttct gcgcatcccg aactccgcgc tgcgattcaa acccgatacg gccgccctgc
     1621 ctgcgaaacc ggcgacgact acgtctgcgg cgacggacac gacgcagacg acgcaaacgc
     1681 aacccacgca aacgcaaacc gccgcgacaa ccggcggccc aacatccgac caacgcgccg
     1741 agcgcaggcg ccagcggatg gaagccggcg gagcgacgag cacgatgagt ccggaagaac
     1801 gtgccgcacg ttggggaaat cgcgctcgtg gaatgcaggg cgcggcaggc gccctcggcg
     1861 gcgctcctcc gatgcgcaag cgtccgcaaa cggtctacat gatggatgct gacggcaagc
     1921 tcaaaccggt cgagatccgc accggcatca ccgacggtca ctacacgcag gtcgtctccg
     1981 gcgacctgaa ggttggcgac atgatcatcg tcggcctggc tacttcgaaa gtcgagacga
     2041 acgcgcctcc cggaagcgcc ggcccgatgg gcggcggcgc gcgtcccggt ggcggcagag
     2101 gtggccggta gtgccgaaaa aggtcattga gattcacgga ctgacaaagg tctattcgat
     2161 gggcgaggtc gaggtgcacg cgcttgcggg cctcgacctc gaggtcgatg agggggagtt
     2221 cctcgccgtc atggggccgt ccggctccgg caaatcgacg ctcatgaaca ttctcggctg
//
LOCUS       DATLVW010000436        18324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_515574, whole genome shotgun sequence.
ACCESSION   DATLVW010000436 DATLVW010000000
VERSION     DATLVW010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18324
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515574"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VNN25_13165"
     CDS             complement(<1..490)
                     /locus_tag="VNN25_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92525.1"
                     /translation="MRDQGGRAAVWLFVVLVAIVYGLVIAEVGRAEYSLVDDYRAFTL
                     LHNGEARAVLWRTLADWRASDAWLYRPFPDTISYALASVLPSHPGVWRVILILIRLGC
                     AGVVFAIARACTPSLAAAAAAAAYFAFFPAIPELHLLRVEGWLTLTLSIVTLNWIRFE
                     RGG"
     gene            513..1682
                     /locus_tag="VNN25_13170"
     CDS             513..1682
                     /locus_tag="VNN25_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXH92526.1"
                     /translation="MLRVGLVSVDPGRKWIGGRYYLQHLVRAVAALPASERVALADVW
                     WMEAQDDDPFAEVRELLDGQQVLGFPTTGIGRVVRALRRAWCGWRDARDLFIDARIDV
                     LFPIAPISEPGIPLVFWMPDFQPWRMPELFSPELQHWYDEHYTRNGVEARLIVLSSND
                     GLRDLETYLPQFRGKARVLHFCSMPTDEWWLRDPVTVARAYGLPDKFFLLANQFSHHK
                     NHPVVFEAVRRLRARGVPVVVACTGSTFGFRGNDYLESIEAFLSEHDLKGAVRILDLI
                     PRADQIALMRRAIAILQPSQFEGWSTVVEDAKTLGKPILVSDLPVHREQAPPGGVFLP
                     VDDAEAWAEAMENAWNVRPPGPNEDEEAAGASAVSRAQVETGRTFVRIVREAAGR"
     gene            1682..2842
                     /locus_tag="VNN25_13175"
     CDS             1682..2842
                     /locus_tag="VNN25_13175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH92527.1"
                     /translation="MRILFCSQAAHTGGGVEAWMEALTSALLARGHEVHTALAKGRFH
                     DPARYVTRHTVANPIEVEGTDTTREVRIANLLRVFERVQPDIIIPVNLADALYAASYA
                     KTKGSTSRIALCIHSQGEDRIDQVRSCAPFIDLAVSVSRRVALQLEGIVPNVRHIPTG
                     VPLPPAPQRKRERIEQIAYVGRLDDGEKRVLDLIPLMHELDGVTLHIAGSGPEEARLR
                     EGLRGMPVVFHGDLSRAELYEKIYPNIDALLVFSPAEAGPIVAWEAMANGIVPIVSDF
                     VGREEEGVLRDRVFPVGDMVASAKLIREPPPARVELPAAYTLPAFEQSWHDALSTLTA
                     MPIRRGPSAILPPLVSPGRLTRLGLGLEGMARIRRLLGHHFEHQDPGSEWPH"
     gene            2842..3702
                     /locus_tag="VNN25_13180"
     CDS             2842..3702
                     /locus_tag="VNN25_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92528.1"
                     /translation="MRLFVGMTTWNSASFLPASLTALRAMTDARETQLVVLDNDSTDG
                     TADIARRFGAEVIVRRSGQAHALMDLFNRSRAEFTLLLHADVVLIDRRWLDACAAHLT
                     GNVALVSPEDIGCGPYTRPWGRGMPESSFLLFRTALARRTRVWFRRRRFKLPVPFRGL
                     DVTGEHITYNIPTRLREHGLEWKAMRVHTSTRLDDPIYTPHFHAPQWTSELAMYRYGL
                     GNFYSIDGIVTHYHNWFERAVEDVADDSERTLPASSGGLPLAFLKRYTRTFLDDFARG
                     AVDIPEASRG"
     gene            3699..4511
                     /locus_tag="VNN25_13185"
     CDS             3699..4511
                     /locus_tag="VNN25_13185"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXH92529.1"
                     /translation="MIGIAALAVVIVALAYVMRRKHRETWTEIFRLRGTIESLQRQLV
                     ASRKEIVLMATSRSMRLPPLMPSQNGEDVVLWDLFERRREGFYVDVGGYDGVGFSNTY
                     FFEAIGWSGIVIEAVPELYKRCAASRPHSTVVHAACGAASGSVAFTVADGPRGVATLS
                     SMTPDHTRIAKEGGSVRTVEVPLRTLDDILAGITEPIDFVSIDVEGAELEVLRGFDLE
                     RFRPRLLVIEDNSAGADRSVANWLAERGYLATQQLEQNIFYTRRDELAEETR"
     gene            4508..5764
                     /locus_tag="VNN25_13190"
     CDS             4508..5764
                     /locus_tag="VNN25_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92530.1"
                     /translation="MKRGWIALALFALSLTLYVYHYRSRFWAAGGDTTPAELLPLAIE
                     RNHSLYFDGLETPGAYWFHHIEGHAVSGYPIIPGFFNIPAYRIAEWRGIPLDVVHRSM
                     LSMISASIVTAASVAFFFLALSRIVTQRATAIGGTLVYAFATTAMSVAARGMWQHGPS
                     LLFLTLALWLLTRGDSLSIALSGLPLSFAVFNRPVNLLIVAPLAVFVLWRHRRAFLPF
                     CAAAAVPAALLAWYSHHYWGTVANLGQYPAGNWFLGRFGAGLAGLLLSPNRGLFVFTP
                     LFLFSLAYTFFVLRHPTKEPLLTALTVGVLLTILLYAKWYSWWGGTSFAYRLLTECVP
                     ALTILLALGWERLFARSRLAQAALIVLAAASFYVHYLGAFYYPCGFDTIPNEINAHPE
                     RLWDVRHGEIERCSSKLFERLSRRLN"
     gene            5821..6807
                     /locus_tag="VNN25_13195"
     CDS             5821..6807
                     /locus_tag="VNN25_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92531.1"
                     /translation="MRILPTYLRPAIGVLLALLAFELLLRHLSWNPPISEGGRESTVV
                     YRLLEGWGVSHWDSHGIRTSPSQQAGPVVLAIGDSFTESAQVNDDEVFTARLQSLLGI
                     RVLNAGRSSLSPADYLLDAPKLRSEFRPSWTIIELNAPDLSDDGFVPTKAHFEVHEGR
                     LVPMPPPAVHLGRTSRILARLRPRSALLNYGIARGGMFRALSQMPPLFLAANEGQPAR
                     VAPPELTPPVEDELSLMTEAFGGRLTFFFIPGFSNGEDVVEKRFLARCAFARLSCVDL
                     RATFADFRTRGRSPFGFPNSSFAQGHMNAEGHRAAADLLAHELSSLRRRGLF"
     gene            6794..8239
                     /locus_tag="VNN25_13200"
     CDS             6794..8239
                     /locus_tag="VNN25_13200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXH92532.1"
                     /translation="MVFSSLRFFLFLGAILGITAIPMSHTRKKAVLCASSCVFYAAWD
                     YRYLALLLIISVIDYFCAARITATENIPVRKAWLTASIVSNLAILGFFKYVAFFLANL
                     NGLLAFTGREVPLLRIILPAGISFYTFKSMSYTIDVFRKRIEPCKSRLDYTMFVTFFP
                     DLIAGPIVRASVFLPQMERTIGPTRERLLHGASLFLVGIGKKLLVADHLAVVADAVFS
                     APQNWSCVTTWCGLVAYTLQIYCDFSGYSDMAIGTAKMFGYDLPENFNMPYLAANVAE
                     FWHRWHITLSEWLRDYLYVSLGGNRKGRARTYVNLMLTMLLGGLWHGAGWNFVLWGFL
                     HGFALALHRAWREFFPTARMPRLFAWASTLLFVTLCWVPFRAPDFATTIAILRALFGS
                     GLGHNVWWPVVLAWSIGLVIIGHLAGSFLPARPGSLRWLDADYSSDSISGPYLVFRMR
                     TIAGSFAVAAAILSIYFFGALNASPFIYFQF"
     gene            8271..10091
                     /locus_tag="VNN25_13205"
     CDS             8271..10091
                     /locus_tag="VNN25_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92533.1"
                     /translation="MRDRLRNAGLAAGVAFVLTIIFCLATTPLPMHFDVKTFGALLPR
                     EPFPAGFENVIFVSPDRGWESGHIRQYALSLFPEPLPSVSWIVFAEGNQPSDWEADGS
                     VDRAGMLRGHAPLSLGPKTLILVRDRSTRDELLKRLPAAAASPTMIDDVPRRPMFARP
                     YNRWTLVSFIRLFVFILAGVLVCSYAYTSGGATGATVGLPLFCVVHVTLTYLLGFLTP
                     RSIELALAVESIIGVILQRSPLPRLILTRRNVSIVLLLAIAMVFSAIRLDFDGDVFTH
                     WLPIARAHHARGSHDLPFLRAQFGGGHRVAYPASFPIYLSTLMWVADVPQSTFDLGQA
                     LDRFALLYRFATALLQILLLTAVAMIVGGNRAAIIAAASLAFAFPLLLGRPEAAESFL
                     VPLVGFAAVALEGETSWTRRVGLILAGSLLFIKADSILIVALLLLPWYAMTIVRQRPG
                     IRALVTDGALLAASSLPFIVLRLEQVRLGIVTTFDFKPFTIESLRSGLPSLSLILTTL
                     LKNVLAFSPWLILLAVAATLPFLLVRHRFAEAGVSIGVLLFAAVLGFLYLFSARDVVW
                     HIATSYARVLDSALLIGALHVFVIALRKQPAGFRPSAADR"
     gene            10136..11083
                     /locus_tag="VNN25_13210"
     CDS             10136..11083
                     /locus_tag="VNN25_13210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXH92534.1"
                     /translation="MSLVTTIIPVFNRPVMLREAVASVLAQTYRPIEVIIVDDGSTDD
                     TRRVAGDLAREHAEIRVIHQTNGGPGVAREAGRQAATGEFIQLLDSDDLLEPRKFELQ
                     VRALEEHPECGIAYGWTRMRFRDGSIHPTPWKRTGERIDTLFPAMLQARWWETTTPLY
                     RASVLRAAGAWLPLRNEEDWELDCRIAATGVRLCQVGAWVSEFRTHDEHLSGSHDKAT
                     LRDRAIAHQAIFGHARRAGIGEDAPEMQHFARELFLLSRQCGAAGLAAEAAMLFQLAR
                     QGAGARGPRLQFSLYALLARTLGWTTMGKLSASVDRMRS"
     gene            11080..11952
                     /locus_tag="VNN25_13215"
     CDS             11080..11952
                     /locus_tag="VNN25_13215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92535.1"
                     /translation="MTPRVSVVLPVYNGAASLAATIDSILTQTERNFELVVVDDGSTD
                     ATAAILRSYDDPRIRVIRQENGGITRALIRGCQAARASFIARQDCGDVSHPERLERLL
                     PLLDTCVLAASEVAYVGPEGEALYTTSHASKSIRDSLLHAGIDAIQSLPHHGSAVFRA
                     DAYTRAGGYRAQFYFAQDLDLWMRMAALGDVCISHDVLYEARIDIRAISSLHRREQVA
                     SAALAIALRDGGDPSLAEQAAAIRPRKRAQSRQSEIRALYFIASCLRRKNDPRWRRYA
                     SRAVRVLLLGRSVA"
     gene            11949..12737
                     /locus_tag="VNN25_13220"
     CDS             11949..12737
                     /locus_tag="VNN25_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH92536.1"
                     /translation="MKRPVFDPTWPEEIQEIYRNDLREIWDHDIERHSFHSYQNQLQL
                     YIDIVERYHAESVLDVGCAQGTLALMLAERGKRVTAVDIRQPFLDYAKTRYEYGDIEL
                     LAANVFDRPPLGTFDLVFGNQIIEHLVYPADFLSILTSYVRPGGTVVVSTPNHAYFKS
                     SLPSYTALGDPRAHEHKQFSAGGSGHFFAYTEQELRDASRQAGLDVLEMIYFETPAIS
                     GHFFVRFLHRIVPLPLLKLMDRAALKVAPRTLAHQLCLVARRPE"
     gene            12734..13297
                     /locus_tag="VNN25_13225"
     CDS             12734..13297
                     /locus_tag="VNN25_13225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXH92537.1"
                     /translation="MRVLERARELASVLAGRLRAALLRMRGAQVGGKLRIGARVRIDR
                     PWCVRIGTRADIEHDVFLKCVDDAASLTLGDFVFIGTTSEIDTAHSVTIGAHTLIAPG
                     VFITDHTHNSARGVRLAAQGSRSAPVVIGEDVWLGARSVVLPGVTIGDGAIVGAGAVV
                     TKDVPPNAIVGGVPARIIGERRNIQGS"
     gene            13294..14067
                     /locus_tag="VNN25_13230"
     CDS             13294..14067
                     /locus_tag="VNN25_13230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH92538.1"
                     /translation="MTSVDAREFYNRIDPARWGRELTPESWAVTRRWLDNAVLDREGP
                     VLELGCGRGAMPGIAPRYIGLDLSFPALAAVDEPFPRIAADMEQLPVRSSGIELVVSF
                     AAIEHVPHPERVFEEITRILRPGGVALLAPAWHCRPWAAEGLEFRGYDELTFTQRLRK
                     RLIPFRNAVVWRALFEAPRRLMRELDALRRRPVGFRYHRLTPNLNAYVGTDCDAFTSM
                     DPHAAILFFASRGWEVLSHPGVWQRLASRAEPVVVRKPK"
     gene            14120..15301
                     /locus_tag="VNN25_13235"
     CDS             14120..15301
                     /locus_tag="VNN25_13235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH92539.1"
                     /translation="MFRLAILASHVIQYQDPFFRLLASDPEIDLTVIYCSLQGAKPYR
                     DEDMKTTLSWDLEMLQGYPWRALRNFGRGSGYWRLVNPGVMPAIWRGRYDAVLLMLGW
                     GTITSLLAVAACRAARIPIAMFGDSSFVPPEDTVMQTLRAGFFRGLFRLTDAFMISGT
                     FNAAYYRHYGADPRRFFDLPWAIDNERFADAAQFAPGEREELRARYGIAPESVVFLFS
                     AKLIPRKDPMSLVRAFEQMPHRGRAALVYMGEGELRPELEMYVRTHDLRNVHLIGFVN
                     QSEIPKHYAMADTFVLPSLDDPRGTVINEAMACGLPVVVTDRCGPVGDIVRHGDNGFV
                     FRPGDIETLATDLDLLASDDSLRQRMSERSREIIADWSYTRGVDGVKAMLRWIAAGEL
                     Q"
     gene            15298..16158
                     /locus_tag="VNN25_13240"
     CDS             15298..16158
                     /locus_tag="VNN25_13240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92540.1"
                     /translation="MITIAIPTYNRGAIVVETLRRLFALTPPADAIIVVDQSPEANPE
                     LAHWQAEGKIRHLRLDAPSIPHAMNVALQEASTPLVLFLDDDIEPSPTLIAAHTESHR
                     DHRATAVVGQILQPGEVPMHIDQPVDDLEFRFHSDRGRFLTNVMAGNLSVDRERALAI
                     GGFDENFIGAAYRFETEFAIRLAAAGGTIWFEPRASIHHLKLATGGLRSFGDHRSTAS
                     PVHSAGDYYFAILHRPPLWRYALTRIRRNVATRYHLRHPWAIPTKLVGEWRGLLLARR
                     LARRGPRLLS"
     gene            16220..17629
                     /locus_tag="VNN25_13245"
     CDS             16220..17629
                     /locus_tag="VNN25_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92541.1"
                     /translation="MITVIFGAILMLSVTVAMTDWRRGWLLAILCGILQDPARKLTPG
                     TPVVMTLSIIVVYTMVLVSAQSTLQAHAREFSRRFSAVKVSAALVFLFLILAAINGLA
                     TFGLENWKAPALAMLVYCAPIPAVLLGFAYLQREEQMFHFFRFYAVLTSIAMIGTFLE
                     YFNVPWRVLGTVALSEFNLRYLPGLEIRMLSGFYRAPDIMGWHAATLTIIGIVMTLRA
                     RFVRAAWPWMLVTAWGFLNCLMSGRRKATYMVAVFAAAFLFRYIRRLTTTQVMTIALA
                     GLALFLVVHKVGQDEDASVYTRGTVTSTSEVLERLEGGLVGTVQQFGLMGAGLGTATQ
                     GVYHVVTNQNLLIGWQEGGLGKLTMELGLPGLFAVALFAAAVFLFMLRLTGLPDVAGS
                     SQFLRAALFGIVIADVVEFFVSAQAYSDAGLTLLSAFFLGCLFATALLDERLANSASA
                     QAVPPVPMQRPFQAPGASF"
     gene            17703..>18324
                     /locus_tag="VNN25_13250"
     CDS             17703..>18324
                     /locus_tag="VNN25_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92542.1"
                     /translation="MRLLHVVPTYFPATRYGGPIYAVHGLCRALAARGHEVDVFTTNV
                     DGGRDSDVPLGVPVTLDGVHIHYFPSPSAALAGLSRRIYRSPDMRVALAERIHYYDLL
                     HLHSVFLWPTFVAARAAERARIPYVVSPRGMLVTELIRRKSPLVKRAWIRLIEKHTFR
                     RASAVHFTAQREWDDARLISIPLPAPFIVPNGIDLPPPSPPARLDDT"
BASE COUNT         3009 a         6016 c         5542 g         3757 t
ORIGIN      
        1 gcccaccgcg ctcgaagcgt atccagttca gggtcacgat cgacagtgtt agcgtcagcc
       61 aaccttcgac gcgcaggaga tgaagctccg ggatggccgg gaagaatgca aagtacgcgg
      121 ccgcagcggc tgcggccgcg agtgacggcg tacatgctcg cgcgatcgcg aagacgacgc
      181 cggcgcaacc caatcggatg aggatgagga tgacgcgcca cacgcctgga tggctaggca
      241 gtaccgaagc gagtgcgtat gagatcgtgt ccggaaatgg ccggtacagc caggcgtcgc
      301 tcgcgcgcca gtccgcgagc gtccgccaca acacggcgcg tgcctcgccg ttgtggagca
      361 gcgtgaatgc gcgatagtcg tctaccagcg agtactcagc gcgcccgacc tccgcgatca
      421 cgaggccgta gacgatcgct acgagcacga cgaagagcca cacggcggcc ctgcctccct
      481 gatcgcgcat acgtagcggg tattctacgg gtatgttgcg cgtgggcctg gtgagcgtcg
      541 accctggacg gaagtggatc ggcggacggt attacctgca gcacctggtg cgcgcggtcg
      601 ccgctctgcc agcttcggag cgcgtcgcac tggcggacgt ctggtggatg gaagcgcagg
      661 atgacgaccc gttcgccgag gtgcgggagc tgctcgacgg acagcaggtg ttggggttcc
      721 caaccacggg catcggccgc gtggtgcgcg ccctgcgacg cgcctggtgc ggatggcgcg
      781 acgcgcgcga cctattcatc gatgcacgca tcgacgtcct ttttcccatc gcgccgattt
      841 ccgagccagg cattccactc gtcttctgga tgcccgactt tcagccgtgg cgcatgccgg
      901 agctcttcag tccggagtta cagcactggt atgacgagca ctacacgcgc aacggtgtgg
      961 aagcgcggct catcgttctc tccagcaacg acggcctgcg cgatctggaa acctacctgc
     1021 cgcaattccg cggcaaggcg cgcgtgctcc acttctgcag catgccgacg gacgaatggt
     1081 ggctgcgtga tccggtcact gttgcacggg cgtacggact gcccgataag ttctttctcc
     1141 tggcgaacca gttctctcat cacaagaacc acccggtcgt cttcgaggcc gtgcgccgcc
     1201 tgcgcgcgcg tggcgtgccg gtcgtcgtgg cctgcacggg cagcacgttc ggctttcggg
     1261 gaaacgacta tctcgaatcg atcgaggcct tcctgagcga acacgatttg aagggcgccg
     1321 tgcggatcct cgatctcatc ccgcgcgcgg accagatcgc gctcatgcgc cgcgcgatcg
     1381 ccattctgca gccgtcgcag ttcgaagggt ggtcgaccgt ggtcgaggac gcgaagactc
     1441 tcggcaagcc gatcctcgtc agcgaccttc ccgttcatcg cgagcaggcg ccgcccggcg
     1501 gcgtcttcct gccggtcgac gacgccgaag catgggcgga ggcgatggag aacgcgtgga
     1561 acgtgcggcc acccggcccg aacgaggatg aagaggcggc cggcgcaagt gccgtgtcca
     1621 gagcccaggt cgagacggga cgaacattcg tcaggattgt gcgcgaggcg gcggggcgtt
     1681 gatgcgcatc ctcttctgtt cgcaggcagc gcacacgggc ggtggggtgg aagcgtggat
     1741 ggaagcgctc acctcggcac tgctcgcgcg cggccacgaa gtgcacaccg cactggcgaa
     1801 agggcgcttc cacgatcccg cgcgctatgt cacacggcac acggtggcga atccgattga
     1861 ggtcgaaggc acagacacca cgcgcgaagt gcgcatcgca aaccttctgc gcgtattcga
     1921 gcgcgtgcag ccggacatca tcattcccgt aaaccttgcc gacgctctct acgccgcttc
     1981 gtacgcgaag acaaaaggct cgacctcacg catcgcactc tgcattcaca gccagggaga
     2041 agaccgtatc gatcaagtgc gctcgtgtgc gccgttcatc gatctggcgg tcagcgtgtc
     2101 gcgccgcgtg gcattgcagc tcgaagggat cgtgccgaac gtgcggcaca tcccgacggg
     2161 ggtcccgtta ccccctgctc cccagcggaa acgcgagcgt atcgagcaga tcgcctacgt
     2221 cggacgtctc gacgatggcg agaagcgcgt cctcgatctg atccccctga tgcacgagct
     2281 cgacggtgtc acgctacaca ttgccggcag cggccctgag gaagcgcggc tacgcgaggg
     2341 tctgcgcggc atgccggtgg tctttcacgg tgatctgtcg cgagcagagc tctacgagaa
     2401 gatctatccg aacatcgacg cgcttctggt gttctcgccg gccgaggcgg ggccgatcgt
     2461 ggcgtgggaa gcgatggcca atggaatcgt accgatcgtc tcggactttg tcggacggga
     2521 agaggagggg gtcctgcgcg accgcgtctt ccccgtcgga gacatggttg cgtcggcgaa
     2581 gctcatccgc gagccgcctc ctgcgcgcgt cgaacttccc gcggcctaca cgcttcccgc
     2641 attcgagcag tcgtggcacg atgcgctgtc cacgctcacc gccatgccga ttcgccgcgg
     2701 tccgtccgcc atccttccgc cactcgtttc tcccgggcgc ctgacgcgcc tcggactggg
     2761 cctcgaaggg atggcccgca ttcgtcgtct cctcggacac cattttgaac atcaagatcc
     2821 aggttcggag tggccacatt gatgcgcctt ttcgtcggca tgacgacatg gaacagcgcg
     2881 tcgtttcttc cggcgtcgct gacagcattg cgagccatga ccgatgcacg cgaaacacag
     2941 cttgtcgttc tcgataacga ttcgacggac ggcacagctg atatcgcgcg ccgcttcggt
     3001 gccgaggtca tcgtgcggcg ttcgggtcag gcgcacgcgc tcatggacct tttcaaccgc
     3061 tcccgcgccg aattcacgct gctcctccat gccgacgtcg tcctcatcga ccgtcgttgg
     3121 ctcgacgcct gtgccgcaca cctcactggc aacgtggcgc tggtgagtcc cgaggacatc
     3181 ggatgcggtc cgtatacgcg gccatggggc cgcgggatgc cggagagctc attccttctt
     3241 tttcgcacag cactggcacg tcgtacacgg gtctggttcc ggcgacgacg gttcaagttg
     3301 cccgttccat tccggggcct cgacgtgaca ggtgagcaca tcacctacaa cattcccacg
     3361 cggttgcggg agcacggact ggaatggaag gccatgcgcg tccatacatc aacgcgtctt
     3421 gatgatccga tctacacacc gcatttccat gctccgcaat ggacctcgga actagcgatg
     3481 taccgctacg gtctcggcaa cttctacagc atcgacggca tcgtcacgca ctaccacaac
     3541 tggttcgagc gggccgtgga ggacgtcgcg gacgactccg agcgcacgct accagcatca
     3601 tcgggcggcc tcccgctggc gtttctcaag cgctacacac gcacgttcct tgatgacttc
     3661 gcgcgcggcg cggtcgacat cccagaggcc tcgcgcggat gatcggcatt gcggcactgg
     3721 ccgtggtgat cgtggccctc gcgtacgtga tgcgccgcaa gcaccgcgaa acatggaccg
     3781 agatcttccg gcttcgcggc acgatcgaat cgttgcagag gcaactcgtc gccagccgca
     3841 aggagatcgt gttgatggcg acctcccgct cgatgcgcct gccgccgctg atgccgtcgc
     3901 agaacggcga ggacgtcgtt ctctgggacc tcttcgaacg gaggcgtgaa gggttctacg
     3961 tcgacgtcgg cgggtacgac ggcgtcggct tcagtaacac ctactttttc gaggccatcg
     4021 gctggagcgg catagtcatc gaggccgtac cggagctgta caagcgctgc gcagcctcgc
     4081 gccctcacag cacagtcgtc catgcagcgt gcggagcggc gagcggaagc gtcgcgttca
     4141 cggtcgctga cggcccgcgc ggcgtcgcaa cgttgtcgtc gatgacgccg gaccacacgc
     4201 gcattgcaaa ggaaggaggc agcgtgcgga ctgtcgaagt cccgctgcga acgctcgacg
     4261 acatccttgc cggcatcacc gagccgatcg actttgtcag catcgatgtc gaaggggcgg
     4321 agctcgaggt gctgcgcgga ttcgacctgg agcgtttccg gccgcggctt ctcgtcatcg
     4381 aggacaactc cgccggagcg gaccgcagcg tggccaactg gctcgcggag cgtggatatc
     4441 tcgccacgca gcagctcgag cagaatatct tctacacacg cagagacgag cttgccgagg
     4501 aaacacgatg aaacgcggct ggatcgccct tgcgctgttc gcgttatcgc tgacgcttta
     4561 cgtctatcac tatcgctcgc gtttctgggc cgctggcggc gacacgactc cggccgagct
     4621 actgccgctg gccatcgagc gcaatcacag cctctacttc gatggcctgg agacgccggg
     4681 cgcgtactgg tttcatcaca tcgaaggaca cgcagtttcc ggctatccga tcatcccagg
     4741 gttcttcaac atccctgcct accgcatcgc ggagtggcgc ggtattccgc tcgacgtcgt
     4801 tcaccgctcg atgctttcga tgatctcggc cagcatcgtc accgcggcct cagtggcgtt
     4861 cttctttctt gcgctctcgc gcatcgtgac ccagcgcgcc accgctatcg gcggcacgct
     4921 cgtctacgcg tttgctacca ctgcgatgag cgtggctgcg cgtggcatgt ggcagcacgg
     4981 accatccttg ctctttctga cgctcgcgct ctggctgttg acgcgcggcg attcgctttc
     5041 gatcgcgctc tccggactcc ccctcagctt cgccgttttc aatcgccccg tgaatctgct
     5101 gatcgtcgca ccgctcgccg tgttcgtcct ttggcgccac cgccgtgcct tccttccgtt
     5161 ctgcgccgcg gccgcagtgc cagcggcgct cctcgcctgg tactcgcacc actactgggg
     5221 cacggtcgcg aacctcggcc agtacccagc cggcaactgg ttcctgggca ggttcggagc
     5281 gggactcgca gggttgctgc tcagcccgaa ccgcggcctg ttcgtcttca cgccgttgtt
     5341 tcttttcagt ctcgcctaca catttttcgt cctgcgccat ccaacaaagg agccactgct
     5401 gaccgcgctc acggtcggcg tcctgctcac aattcttctt tatgcgaagt ggtactcatg
     5461 gtggggcggg acttccttcg cctaccggct gctgaccgag tgcgtgccgg cactgacgat
     5521 cttgctcgcg ctcggatggg aacggctgtt cgcccgaagc aggctcgccc aggccgcgct
     5581 gatcgttctc gctgccgctt cgttttatgt ccactacctc ggtgcgttct attatccctg
     5641 cggcttcgac accattccaa acgagatcaa cgcccacccc gaacgcctgt gggatgttcg
     5701 ccacggcgag atcgagcgtt gctcatcgaa actgttcgag cggttgtcca ggcgtctgaa
     5761 ctgatggggg ctcgtttggg cactcatcaa gatgctgtgt caaaatcgaa gtcacggccg
     5821 atgcgcatcc taccgaccta tcttcgccct gccatcggcg ttcttctggc cctgctggcc
     5881 tttgaacttc tcttacggca cctctcgtgg aaccctccga tttcggaggg tggccgcgaa
     5941 tcgacagtgg tttatcgcct tcttgaaggt tggggcgtca gccactggga ctcccacggt
     6001 attcgcacaa gtccgtcgca acaggccgga ccagttgtac tggccatcgg agactccttc
     6061 accgagagcg cccaggtgaa cgacgatgag gtttttacgg cccgactgca gtccctgctc
     6121 ggcatcagag ttctcaacgc cggacgctca tcgctttcac cggccgacta tcttctggat
     6181 gccccgaagc tacgttctga atttcgaccg tcgtggacga tcatcgagct caacgcaccg
     6241 gatctgtcgg atgacggatt cgtcccgacg aaagcgcatt tcgaggttca cgaaggacgg
     6301 ctggttccca tgcctccgcc cgccgtccat ctcggccgaa cgtcccgaat acttgcccga
     6361 ctccggccac gcagcgcatt gctgaactat gggatcgcgc gtggcggtat gtttcgtgcg
     6421 ctttctcaaa tgcctcctct ctttcttgcc gccaacgaag ggcagccagc ccgtgtcgcg
     6481 ccgccggagc tcacgccacc cgtggaagac gagctgtcgt tgatgaccga agcgtttggg
     6541 ggccggctga ccttcttctt catccccgga ttcagtaacg gggaggacgt cgtcgagaag
     6601 cggtttctcg cacgctgtgc gttcgcaagg ctgagctgcg tcgacctgcg agctacgttt
     6661 gcggactttc gcacccgcgg ccgctcacca ttcggattcc caaatagttc attcgcgcag
     6721 gggcatatga acgccgaagg ccaccgcgcc gcagcggacc ttctcgctca tgagctctcg
     6781 tcattgaggc gccgtggtct tttctagcct ccgcttcttc ctcttcctcg gtgcgattct
     6841 cggtatcacg gcgatcccga tgtcgcacac gcgcaaaaag gccgtgctct gcgcaagctc
     6901 ctgcgttttc tacgccgcct gggactatcg atacctggca ctgctgctga tcatcagcgt
     6961 cattgactac ttctgcgccg cgcggatcac ggccaccgaa aacatccccg tccgaaaggc
     7021 atggctgact gccagcatcg tgtcgaatct ggccattctc ggattcttca agtatgtcgc
     7081 gttctttctc gccaacttga acggtctgct cgcgttcact ggccgcgagg ttccccttct
     7141 ccgcatcatc ctgccggcgg ggatcagctt ctacacattc aaatcgatga gctacaccat
     7201 cgatgtcttt cgaaagcgca tcgaaccgtg caagtcgcgt ctcgattaca cgatgttcgt
     7261 gacgttcttt cccgacctta tcgcgggtcc gatcgtgcgc gcctccgtgt ttctgccgca
     7321 gatggagcga accatcggac cgactcgcga acgtcttctc cacggagctt ctctcttcct
     7381 cgtcggcatc ggaaagaaac tgctcgtcgc cgatcatctt gcggtcgtcg cggatgccgt
     7441 gttctcggct ccgcagaact ggagctgcgt gaccacatgg tgcgggctgg tcgcgtatac
     7501 cctgcagatc tattgcgact tctcgggcta ctccgacatg gccatcggaa ccgcaaagat
     7561 gtttggctac gatctgcccg agaatttcaa catgccttat ctcgcagcca acgttgccga
     7621 gttctggcat cgctggcaca tcactctgag cgaatggctc cgcgactatc tatatgtgtc
     7681 tctcggagga aatcgaaagg ggagagcccg aacgtacgtg aatctcatgc tgaccatgct
     7741 cttgggtggc ctatggcatg gagcgggttg gaacttcgta ctctggggat tcctgcacgg
     7801 attcgcactg gcactccatc gtgcgtggcg cgagtttttc cctaccgcgc gcatgccacg
     7861 cctttttgcc tgggcatcga cgcttctctt cgtgacgctt tgctgggtac cgtttcgtgc
     7921 gcctgatttc gccacgacca tcgcgatact gcgtgccctc ttcggatcgg gcctcggcca
     7981 taacgtctgg tggcccgtcg tgctggcatg gtcgatcggc ctcgtgatca tcggtcacct
     8041 cgccggatct tttcttccgg cgaggccagg ttctctgcgc tggctcgacg ccgattactc
     8101 atcggactca atctcgggac cttaccttgt cttccgcatg cgcacgatcg cgggaagctt
     8161 cgcggtcgcc gccgcgatcc tgtcgatcta ctttttcgga gcgttgaacg ccagtccttt
     8221 catctacttc cagttctgag acccggcgaa ttcgtgcatg atgcgcggat atgcgcgacc
     8281 ggcttcgcaa cgcagggttg gccgctgggg ttgccttcgt cctgacgatc atcttctgtc
     8341 tggcgacgac gccccttccg atgcacttcg acgtgaaaac gtttggcgcg ctcttgccga
     8401 gggagccgtt tccagcgggt ttcgagaacg tcatcttcgt cagccccgat cgcggttggg
     8461 agagcggcca catacgccag tacgcattga gtctgtttcc cgagccacta ccgtccgttt
     8521 cctggatcgt ctttgccgaa ggcaatcaac cgtctgactg ggaagctgac ggttccgtcg
     8581 atcgagcggg catgcttcga gggcacgcac cgctatcttt aggtccgaag acgctcatcc
     8641 tcgtccgcga tcggtcgacg cgcgatgagc ttctgaagag actcccggct gcggcagcat
     8701 cgccaacgat gatcgatgac gtgccgaggc ggcctatgtt cgcgcgtcct tacaaccgct
     8761 ggacgctcgt ttcgttcatc cggctgttcg tcttcattct tgccggtgtc ctggtgtgta
     8821 gctacgcata tacctccgga ggtgctaccg gggcgacggt tggactcccc ctcttctgcg
     8881 tcgttcacgt gacactcaca tatctgctcg gttttctcac gccacgttcg attgagctgg
     8941 ccctcgctgt cgaaagcatc atcggagtga tcctccagcg ctcaccgttg cctcggctga
     9001 tcctcacccg gcgcaacgtg tcgatcgtgc tgctgctcgc gatcgcgatg gtcttcagcg
     9061 ccatcaggct cgatttcgat ggcgatgtct tcacgcactg gctgccgatc gcgcgtgccc
     9121 atcacgcgcg cggatcacat gatctgccgt ttctgcgcgc ccagttcgga ggcggtcatc
     9181 gagtggccta tccggcctcg ttccccatct atctgtcgac gctgatgtgg gtcgccgacg
     9241 tcccacaatc caccttcgac ctcggacagg cgctcgatcg atttgccttg ctctatcgct
     9301 tcgcgacggc gcttctgcag atccttctcc tgaccgcggt ggccatgatc gtcggcggaa
     9361 atcgcgccgc aatcatcgcg gccgcatccc tcgccttcgc cttccctctt ctcctcgggc
     9421 ggccggaggc ggccgagagc ttcctcgtgc cgctcgtagg attcgccgcg gttgcgctcg
     9481 agggggagac gagctggacg cgccgcgtcg ggctgatcct ggccggctcg ttactcttca
     9541 tcaaagcaga ctcgatcctg atcgtggctc tgctccttct gccgtggtac gcgatgacca
     9601 tcgtccgaca acgcccgggc atccgcgcgc ttgttaccga tggggcgtta ctcgcggcat
     9661 cgtctcttcc gtttatcgtt ctccgtctcg agcaggtccg ccttggcatc gtcaccacgt
     9721 tcgatttcaa accgttcacg atcgagtcgt tgcgatcggg actgccgtcg ctgtcgctta
     9781 tcctgacaac gcttctcaag aatgttctcg cgttctcgcc gtggctgatt ctcctggcgg
     9841 ttgccgctac tctgcccttt ctcttggtac gacaccgctt cgccgaggcc ggcgtgtcga
     9901 tcggtgtgct cctctttgcc gctgttctcg gatttcttta tctgttctcc gcgcgtgacg
     9961 tcgtctggca catcgcgacc tcatacgcgc gcgtgctcga cagcgctctc ctgatcgggg
    10021 cgttgcatgt gttcgtgatc gcgcttcgca agcagcccgc cggcttccgg ccgtcagcag
    10081 cagatcgttg acaggctgat cgggacggaa cgcgcacgcg gtctacaatc cgacggtgtc
    10141 tctcgttacg acgatcatcc cggtcttcaa ccgcccggta atgttgcgcg aggcggtggc
    10201 cagcgttctg gcgcagacgt accggcccat cgaagtcatc atcgtcgacg atggatcgac
    10261 cgacgacacg cggcgcgttg ccggcgatct cgcgcgcgag cacgccgaga tacgtgtcat
    10321 tcaccagacg aatggcgggc cgggcgtggc gcgggaagcg gggaggcagg cggccaccgg
    10381 cgaattcatt cagttactcg acagcgatga tctgctcgag ccgcgcaagt tcgaactgca
    10441 ggtccgtgcg ctggaggagc atcccgagtg tggcatcgcg tacggctgga cacggatgcg
    10501 attccgcgac ggcagcattc atccgacacc gtggaagcga accggcgagc gcatcgacac
    10561 cctgttcccg gctatgttgc aggctcgatg gtgggagacg acgacgccgc tctaccgcgc
    10621 gtcggtcctg cgtgcagcag gagcgtggct gccattgcgc aacgaagagg actgggagct
    10681 tgattgccgg atcgccgcaa ctggagtgcg cctgtgccag gttggagcat gggtctccga
    10741 gttccggacg cacgatgagc atctctccgg ctcgcacgac aaagcgacgc tccgcgaccg
    10801 ggcaatcgcc caccaggcga tcttcggtca cgcgcggcgc gccggcatcg gcgaagatgc
    10861 gcccgagatg cagcacttcg cgcgcgagct gtttctcctc tcgcgtcagt gcggcgcggc
    10921 ggggctggcg gcggaggcgg cgatgctctt ccaactggcg cggcaaggcg caggggcgcg
    10981 aggcccgcgt ctacaattca gcctttacgc gcttctggcg cgcacattag gatggacaac
    11041 gatgggaaag ctgtcagcga gcgtggaccg catgcggtcg tgacgccgcg agtgtccgtg
    11101 gtccttcccg tgtacaacgg tgccgcatcg ctggcggcca cgatcgacag catcctcacg
    11161 caaacggagc gtaacttcga gctggtcgtc gtcgacgatg gctcgacgga cgcgacagcg
    11221 gcgatcctgc gcagctatga cgatccgcgc atacgcgtca tcaggcagga gaatggcggc
    11281 atcacgcgcg cactcatccg cggatgccag gctgcgcgcg cttccttcat cgcgcggcag
    11341 gattgtggcg atgtctcgca tcctgagcgg ctggagcgtc ttctgccgct tctcgatacg
    11401 tgcgttctcg cggcctcgga ggtcgcgtac gtcggccccg agggagaggc gctgtatacg
    11461 acgtcgcacg catcgaagag tattcgtgac tcgctcctgc acgccggtat cgacgccatt
    11521 cagtcgctcc cgcatcacgg cagcgcagtc ttccgcgccg acgcgtatac gcgcgctggc
    11581 gggtaccgcg cacagttcta cttcgcccag gatctcgatc tctggatgcg catggccgcc
    11641 ctcggcgacg tctgcatcag ccacgacgtt ttgtacgaag cgcgcatcga catccgcgcc
    11701 atcagcagtc tccaccgccg cgagcaggtc gcatctgcgg cgcttgccat cgcgttgcgc
    11761 gatggcggcg atccctctct ggccgagcag gccgcggcga tccggccccg gaagcgcgca
    11821 caatcgcggc aatcagagat tcgcgcgctc tacttcatcg cctcctgcct gcggcgaaag
    11881 aacgacccgc gatggcggcg ctatgcctcg cgcgccgtgc gcgtcctgct gctcggaagg
    11941 agtgtcgcgt gaagcggccc gttttcgatc ctacatggcc cgaggagatc caggagatct
    12001 accgcaacga cttgcgcgag atttgggatc acgacatcga gcgccactcg ttccactcct
    12061 accaaaacca gctccagctc tacatcgata tcgtcgagcg ctatcacgct gagagcgtgc
    12121 tcgatgtcgg ctgcgcgcag ggaacgctcg cactaatgct cgccgagcgc ggcaaacgcg
    12181 tcaccgcggt cgacatccgg cagccctttc tcgactatgc gaagacccgc tatgagtacg
    12241 gcgacatcga gctcctggcc gcaaacgtct tcgaccgccc tcccctgggc actttcgatc
    12301 tcgtcttcgg aaaccagatc atcgagcacc tcgtctatcc tgccgacttt cttagcatcc
    12361 tgacgtccta cgtgcggccg ggaggcacgg tggtcgtctc cacgccaaac cacgcgtatt
    12421 tcaaatcgag cctgccgagc tacaccgcgc tcggcgatcc gcgggcgcac gagcacaagc
    12481 agttctccgc cggtggcagt ggtcacttct ttgcctacac ggaacaggaa ctgcgtgacg
    12541 cctcgcgtca agccgggctc gatgtgctgg agatgatcta tttcgagacg cctgccatct
    12601 ccggccattt cttcgtcagg ttcttgcacc gcatagtgcc gctgccgctg ctgaaactga
    12661 tggatcgcgc ggcactgaag gtcgcgccac gaaccctcgc acatcagctc tgcctcgtgg
    12721 cgcggaggcc ggagtgaggg ttctcgaacg ggcgcgagag cttgccagtg tgcttgccgg
    12781 acgcctccgg gcggcgttgc ttcgcatgcg cggcgcgcaa gtcggaggga aactgcgcat
    12841 cggcgcgcgt gtgcgcatcg accggccgtg gtgcgtgcgg atcggcacac gggcagacat
    12901 cgaacacgac gtgttcctca agtgcgtcga cgacgcagcg tcactgaccc tcggcgactt
    12961 cgtcttcatt ggaaccacca gcgaaatcga cactgcccat tccgtcacga tcggagcgca
    13021 taccctcatc gctccgggcg ttttcatcac cgaccacacc cacaactccg cacgcggcgt
    13081 ccggctcgca gcacagggga gtcgaagcgc gccggtggtt atcggcgagg atgtctggct
    13141 cggcgctcgc tccgttgtgc tgccgggggt caccatcggg gacggagcaa tcgtcggcgc
    13201 cggcgcggtg gtgacgaaag atgttccgcc gaacgccatc gtaggcggcg tgcccgcgcg
    13261 aatcatcggc gagcgacgca acatccaggg atcatgactt ccgtagacgc acgagagttt
    13321 tataaccgaa tcgaccctgc tcgatggggg cgcgagctga ctcccgaatc gtgggccgtg
    13381 acccggcggt ggctcgacaa cgcggtgctc gaccgggaag gtccggtgct ggagctggga
    13441 tgcggccgcg gagcaatgcc gggcatcgct ccgcgataca tcggtctcga tctctcgttt
    13501 ccggctctgg cggcggtcga tgagccgttt ccgcgcatcg ccgcggacat ggaacaactg
    13561 ccggtccggt ccagtggtat cgagctcgtg gtttcatttg cggcgatcga gcacgtgccg
    13621 catccggagc gcgtgttcga ggagatcacc cggatcctgc ggcccggagg agtcgcactt
    13681 ctcgctcctg cgtggcactg ccggccgtgg gcggccgagg gtctggagtt tcgcggatac
    13741 gatgaactga cgttcaccca gcgcttgcgc aagcgtctca ttccttttcg caacgcggtg
    13801 gtatggcgag cgctgttcga ggctccgcgg cgtctgatgc gtgagttgga cgccttgcgc
    13861 cggcggccgg tggggttccg ataccacagg ctcactccca acctgaacgc gtatgtcgga
    13921 accgactgtg acgcattcac gagtatggat ccgcacgcgg ccattctgtt tttcgcgtct
    13981 cgcggctggg aagtactgag ccatcccgga gtgtggcaac gtcttgcctc cagggcggag
    14041 ccggtcgtgg tccgaaaacc gaagtaaccg tgccggccag gagaacgtac aggagtcctt
    14101 cgtgcgaaga tcgtgaccga tgttccgcct ggcaatcctc gcctctcacg tgatccagta
    14161 tcaggatccg ttcttccgcc ttctggccag cgacccggag atcgatctca ccgtcatcta
    14221 ctgctcgctc cagggagcga agccctaccg cgatgaggat atgaagacga ctcttagttg
    14281 ggacctggaa atgctgcaag gctatccatg gcgggcgctt cgaaacttcg gacgcggcag
    14341 cggctactgg cgcctggtca atcctggagt gatgcccgcg atctggcgcg gccgttacga
    14401 tgccgtgctg ctcatgctcg gctggggcac gatcacctcg ctgctggcgg tcgccgcgtg
    14461 ccgggccgca cgcattccga ttgccatgtt cggcgacagc agcttcgtgc cgccggagga
    14521 tacggtgatg cagacgcttc gcgccggctt ctttcgcggg ctcttccgct tgaccgatgc
    14581 cttcatgatc tcgggcacat tcaacgccgc ctactaccgt cattacggcg ccgacccgcg
    14641 acgattcttc gacctgccgt gggccatcga caatgaacgc ttcgcggatg ccgcacagtt
    14701 cgcgcccggc gagcgcgaag agctgcgggc gcgttacggc atcgcgccgg agagcgtcgt
    14761 ctttctcttc tccgcaaagc tcatcccgcg taaagatcca atgtcactgg tacgcgcatt
    14821 cgagcagatg ccgcaccgcg gccgcgccgc cctcgtctac atgggcgagg gtgagctgcg
    14881 gccggagttg gagatgtacg ttcgcacgca tgatctgaga aacgtccatc tcatcggctt
    14941 cgtgaatcag agcgaaatcc cgaagcacta cgccatggcc gatacgttcg tccttccttc
    15001 gctcgacgat ccccgcggta cagtcatcaa cgaggcgatg gcgtgcggtc tgcctgttgt
    15061 ggtcacggac cgttgcggcc ccgtcggcga catcgtccgt cacggcgaca acggcttcgt
    15121 cttcaggccc ggagacatcg aaaccctcgc gaccgatctc gatctcctcg catccgatga
    15181 ctcgttgcgg cagcgtatga gtgagcgctc gcgcgaaatc atcgccgatt ggagctacac
    15241 gcgaggcgtc gacggagtga aggcgatgtt gcggtggatc gcagcaggtg aactgcaatg
    15301 atcaccatcg ctatcccgac gtacaaccgc ggcgcgatcg tcgtggaaac gctgcggcgg
    15361 ctcttcgcgc tgacgccacc ggcggacgcc atcatcgtcg tcgaccagag tccggaagcg
    15421 aaccctgagc tggcgcactg gcaagccgag gggaagattc gccatctccg cctcgacgcc
    15481 ccctcgattc ctcacgccat gaacgtggcg ttgcaggaag cgtcgacgcc gctggttctc
    15541 tttctggacg acgatatcga accatcgcca acgctgatcg ccgcgcatac ggaatcgcac
    15601 cgcgatcatc gtgcgacggc ggttgtcggc cagatcctgc aaccgggtga agtgccgatg
    15661 cacatcgatc agccggtgga tgatctcgag tttcgctttc actccgatcg tggacgattc
    15721 ctcaccaacg ttatggccgg caatctctcg gtggaccgcg aacgtgcctt ggccatcggc
    15781 ggcttcgatg agaacttcat cggcgccgcg taccgcttcg agaccgagtt tgccattcgc
    15841 ctggccgccg caggcggcac gatctggttc gagccgcgcg cttccatcca tcatctgaag
    15901 ctcgctacag gcggtctgcg ctccttcggt gaccaccgtt ccacggcctc tcccgtccac
    15961 tcggccggcg attattactt cgccatcctc catcgccccc cgctctggcg ttacgccctg
    16021 acgcgcattc gccgcaatgt ggcaacccgc tatcacctcc gccatccctg ggccatcccc
    16081 accaagctcg tcggggagtg gcgcggcttg ctgctcgcgc gaaggctggc gcgccgtggg
    16141 ccgaggttgc tgtcttagaa agcatctccg gagcgcgacg aaatgctgca cgacggcccg
    16201 gcgttcacga agccggccca tgatcaccgt catcttcggc gcaattctca tgctgtcggt
    16261 caccgtcgcg atgaccgatt ggcggcgcgg ctggctgctg gcgatcctct gcggcattct
    16321 gcaggatccg gcgcgcaagc tcacccccgg cacgccggtg gtcatgacgc tgagcatcat
    16381 cgtcgtctac acaatggtcc tggtctccgc gcagtcgaca ctccaggccc acgcgcgcga
    16441 gttctcgcgg cggttcagcg ctgtcaaggt cagcgcggcg ctcgtgttcc tcttcctcat
    16501 cctggccgcg atcaatggcc tggcgacgtt cggactggag aactggaagg cccctgctct
    16561 ggcgatgctc gtctactgcg cgcccatccc ggccgtgctc ctcggcttcg cctatttgca
    16621 gcgcgaagaa cagatgtttc acttcttccg cttctatgct gtcctgacgt cgatcgccat
    16681 gatcgggacg ttcctggagt acttcaatgt gccttggcgc gtcctcggca ctgttgccct
    16741 ctccgaattc aacctgcgct acctgccggg gctggagatc cggatgctct ccggcttcta
    16801 ccgtgcgccg gacatcatgg gatggcacgc ggcgacgctg accatcatcg gcatcgtcat
    16861 gacgctgcgc gcgcgtttcg tgcgcgcggc gtggccatgg atgctcgtca cggcatgggg
    16921 ctttctgaac tgtctgatga gcggccgtcg caaggctacc tacatggttg cggttttcgc
    16981 ggcagccttt ctgtttcggt atatccggcg tctgacgact acgcaggtga tgacgattgc
    17041 cctcgcgggc ctggctctat tcctggtggt gcacaaggtc gggcaggatg aggacgccag
    17101 tgtttacacg cgcggtactg tgacgtcgac ctccgaggtc ctcgagcggc tggagggtgg
    17161 ccttgtcggc acggttcagc aattcggctt gatgggtgcc ggcctcggta cggcaaccca
    17221 gggcgtgtac cacgtcgtta ccaatcagaa cctgttgatc ggctggcagg aaggagggtt
    17281 ggggaaactg acgatggagc tcggcctccc cggcctcttc gccgtggcgc tcttcgctgc
    17341 tgcggtcttc ttgttcatgc tgaggctcac gggacttccc gacgtggccg gctcatcgca
    17401 gtttctccga gcagcgctct tcggcatcgt gatcgccgac gtcgtcgaat ttttcgtatc
    17461 cgcgcaggcg tattcggacg caggtttgac gttgctcagc gccttcttcc tcggctgcct
    17521 tttcgccact gccctactcg acgaacgcct cgccaacagc gcgtccgcac aggcggtgcc
    17581 gccggtgccg atgcaacgtc cgtttcaagc tcccggcgcg agcttctgag attcgaggat
    17641 aggcggcggc gaaaccgaac ggccggcgag agcaaacaaa catccggtat acgctttgtg
    17701 cggtgcgtct ccttcacgtc gttcctacgt atttccccgc gacccgatac ggcggcccga
    17761 tctacgccgt ccacggacta tgccgggcgc tcgctgcgcg tggacatgaa gtcgacgtct
    17821 tcacgacgaa tgtcgacggc ggccgcgact ccgacgtccc cctcggcgtt cctgtcactc
    17881 tcgacggtgt ccacatccac tactttccct cgccatcagc agcgttggcc ggtctgagcc
    17941 ggagaatcta tcgctcgcca gacatgcgcg tcgcgctggc tgagagaatc cattactacg
    18001 atctcctgca tctgcactcc gttttcctct ggccgacgtt tgtagcagcc agggccgcgg
    18061 agagagcgcg catcccgtac gtcgtctccc cgcgcggcat gctcgtcacg gaactgatcc
    18121 gccggaagag ccctctcgtc aagcgcgcat ggatccggct catcgagaaa cacaccttcc
    18181 ggcgcgcgtc tgctgtccat ttcaccgcgc aacgggagtg ggatgacgct cgcttgattt
    18241 cgatcccgct tcctgcgccg ttcatcgtgc cgaatggaat cgaccttcct ccaccatcgc
    18301 ctcccgcgcg tctcgacgac actc
//
LOCUS       DATLVW010000437         3880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_389441, whole genome shotgun sequence.
ACCESSION   DATLVW010000437 DATLVW010000000
VERSION     DATLVW010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389441"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VNN25_13255"
     CDS             <1..395
                     /locus_tag="VNN25_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92543.1"
                     /translation="SESEKPDLEPLCAALVANVIAEGGEIISAHALFLFDAALQTEDA
                     NVIRRVANHLSDIGGVVTSTSTPHTPHPTPRPTPYNFARDYGACLKAHAVAKRLRARF
                     PNADFEGMLAAVHRNLDAAPPSEEMAFA"
     gene            673..2043
                     /gene="dacB"
                     /locus_tag="VNN25_13260"
     CDS             673..2043
                     /gene="dacB"
                     /locus_tag="VNN25_13260"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HXH92544.1"
                     /translation="MRSSTFVLAALLFASCATAPSPQSPGQSLAQRIEAITSKPSLAG
                     AIWGIDVVDDRGNTIYARNEGTLLIPASNRKLFAAATVASCEGLDRRIATEFWSDGSD
                     IIIRGGGDPSFGGRWAFDRDAVFAPVVNALRSRNVTDVNNVIADVSLFDRTTIPGSWK
                     VGNLGSDYATPVDALAYNENVIGVVADHCAGPVITMDPAFVPASAWVGCGPDNLTIRS
                     GRGNKVTISGTMPEHVQVLAGIGDPALYAAQALLDALRHAGINVRGHAVVNTTQRAWS
                     ERISVIESPPLWQLLSTVLKPSQNLYAEMLFKGLSASAAQPASYEASEDIERRFLTTE
                     VGINASDFRFVDGCGLSPDDLVAPRAIVTLLRWMNDPNRRGVWSMMLAQPGEEGTLRR
                     RLLPLADRMRGKTGSINGVNALSGILDGPHGTHRYFTIIINHHTAGSSDVLHAIDAIV
                     SEIANW"
     gene            complement(2047..2307)
                     /locus_tag="VNN25_13265"
     CDS             complement(2047..2307)
                     /locus_tag="VNN25_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92545.1"
                     /translation="MPVETYDLIDTTNLSALVANFARVHMLLAMFDGEPDKWIEFFES
                     CGTPEERRRDLPFAEEVKRRMIGDPEYLSRLRRVVGELSELI"
     gene            complement(2377..2778)
                     /locus_tag="VNN25_13270"
     CDS             complement(2377..2778)
                     /locus_tag="VNN25_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92546.1"
                     /translation="MANDVQQLRDLARAYWAGEPVVCPKHPGVIMTGSFVQTTFADHL
                     FLTCPRGRETITIPQRPRQMEFHRQQVEGFLENIERGDANLCYRCQSVLDVESSVNPT
                     TGMAEYSFTCVRCFSYGTWSGQNAELAGVNG"
     gene            complement(2963..>3880)
                     /locus_tag="VNN25_13275"
     CDS             complement(2963..>3880)
                     /locus_tag="VNN25_13275"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXH92547.1"
                     /translation="GRGAARFAYRLIGLIHAEVKRRPDVLTFCDDAAGIRQAKPDGKT
                     ALMIGVEGGHAIEDSLSILRDFYRLGARYMTLTHVNTNNWCDSSGDGGRHNGLTDFGR
                     DVVRTMNDLGMIVDISHVSDKAFYAVLETTRVPLVATHSSCRALCRHPRNMTDDMLRD
                     LAKNGGVCMINFFSAFLNDAVAQVIMNSQKRVKSESAGGTEEMPDDATDWDLYLEWFN
                     TLGSPQATIDDAVDHILHAADVAGIDHVGIGSDFDGVPALPKDLQTAAGLPLLTARLL
                     ERGVSEGDVEKILGGNFMRVFEHIEAGRK"
BASE COUNT          699 a         1210 c         1238 g          733 t
ORIGIN      
        1 ggagtgagag cgagaagccc gacctcgagc cgctgtgtgc cgcgctggtc gcaaacgtca
       61 tcgccgaggg tggtgagatc atcagcgcac atgcgctttt cctgttcgat gctgccctgc
      121 agaccgagga tgcgaatgtg atccgccgcg tcgccaatca tctctctgat atcggcggag
      181 tcgttacttc tacttccaca ccccacaccc cacaccccac accccgaccc accccctaca
      241 acttcgcccg cgactacggc gcgtgcctca aagcgcacgc ggtggcaaag cggttgcgtg
      301 cgcggtttcc gaacgccgat ttcgaaggca tgctggccgc tgtgcatcgc aacctggacg
      361 ctgcgccgcc gtccgaggag atggcatttg cgtagaccct ctcacgcacg aaaattgagg
      421 ttgccgacat tgcgtacacg taaatcgagc gccttcgccc ccacgcttct cagtggggga
      481 gaaggtggcc gaaggcggat gagggggcgt gcccaatgtg cggcatcgtt gcgttctacc
      541 aaccgcttgt gggtctctgt tgtgtgaaac gcccccctca tccgccttcg gcaccttctc
      601 cccccgaaaa gcacgggggg agaaggcgct cgattgagag tgagctgcaa gagttgaaga
      661 ggagtttgag aaatgcggag tagtactttc gttcttgcgg cactcctttt cgcttcatgt
      721 gcgactgcgc catcgccgca atcgcccggg caatcgcttg ctcagcggat cgaagcgatc
      781 actagcaagc cgtcgctggc cggtgcgatc tgggggatcg acgtcgtcga cgatcgtggc
      841 aacacgatct acgcgcgcaa tgaagggacg ctgctcattc ctgcgtcgaa ccggaagctt
      901 ttcgccgccg caaccgtggc ttcgtgcgaa ggactcgatc gaaggatcgc cacggagttc
      961 tggagcgatg ggagcgacat catcatccgc ggtggcggcg atccgtcgtt cggtggacgc
     1021 tgggcattcg atcgcgatgc cgtgttcgcg cctgtcgtga acgcgctccg aagccggaac
     1081 gtcaccgacg tgaacaacgt catcgcggac gtctcactct tcgaccgcac gaccatcccc
     1141 ggcagttgga aagtcggcaa ccttggcagc gattacgcga cgccggtcga tgcgctcgcc
     1201 tacaacgaga acgtgatcgg ggtcgtcgcc gatcactgcg cgggcccggt catcacgatg
     1261 gatcctgcgt ttgtccccgc gtccgcgtgg gtcgggtgcg ggccggacaa cctgacgatt
     1321 cgttccggca ggggcaataa agtcacgatc agcggcacga tgccggagca cgtgcaggtt
     1381 ctcgccggca tcggcgatcc ggcgctttac gcggctcagg cgcttctcga tgcgctgcgg
     1441 cacgccggca tcaacgttcg cgggcatgca gtcgtcaaca caacgcagcg cgcgtggagc
     1501 gagcggatca gcgtcatcga gtcgccgccg ctctggcagc ttctctcgac tgttctgaag
     1561 ccaagtcaga acctctatgc ggagatgctg ttcaagggac tgtcggcgag cgccgctcag
     1621 ccggcgtcgt acgaagcatc ggaggatatc gagcgccgct tcctgacgac ggaggtgggg
     1681 atcaacgctt cggactttcg tttcgtggac ggctgcggac tctcgcccga tgacctcgtc
     1741 gcgccgcgcg ccatcgtcac gctcctgcgc tggatgaacg atccgaaccg gcgcggcgtc
     1801 tggtcgatga tgctcgctca gcccggtgaa gaaggaacgc tccgtcggcg cctgctaccg
     1861 ttggccgacc ggatgcgcgg aaagaccggc agtatcaacg gagtcaacgc gctgagcggg
     1921 attctcgacg gtccgcacgg cacgcaccgc tacttcacga tcatcatcaa tcaccacacc
     1981 gcggggtcgt cggacgtgtt gcacgcgatc gacgccatcg tgagcgagat cgcgaactgg
     2041 tgagattcag atcagctcgg aaagctcgcc gacgacgcgc cggagtctgc tgaggtactc
     2101 cggatcgccg atcatccggc gctttacctc ctcggcgaag ggaagatcgc ggcgccgctc
     2161 ttcgggcgtg ccgcacgatt cgaagaactc gatccacttg tcgggctcgc cgtcgaacat
     2221 cgcaagcagc atgtggacgc gcgcaaagtt tgccaccagc gccgacagat tcgtggtgtc
     2281 gatcaggtca taggtctcaa cgggcacgat gagcgagggt ccccctacca aggagtgact
     2341 gcgggacgga gttccctacc cactcacgcg atttttttat ccattgacgc cggcgagctc
     2401 agcgttctgg ccgctccacg ttccgtacga gaagcagcgg acgcacgtga acgagtactc
     2461 ggccatgccg gtcgtggggt tgaccgacga ttcgacgtcc aggacggatt ggcatcgata
     2521 gcagagattg gcgtcgccac gctcgatgtt ctccaggaac ccttccacct gctgccgatg
     2581 aaactccatc tgccgcggcc gctggggaat ggtgatcgtc tcccgtccgc gcgggcaggt
     2641 aaggaagaga tgatcggcga atgtggtctg gacgaagctg ccggtcatga tgacgccggg
     2701 atgcttggga cacacgaccg gttcgcccgc ccagtacgcc cgcgccagat cgcgaagttg
     2761 ttgtacgtcg ttggccatga gagggagttt agccgactct cgcgctctca tgcgcaacgt
     2821 cgtcctgaca cagcgtcgcg tcgagtggga gtagaaccgt ttcacgcgga gacgcggacg
     2881 cgcggagttt tctttcctcc gcgttccccg cgtctccgcg tgaaacggtt ttgattcttg
     2941 agcagtgccg acaagccgcc acctacttcc tccccgcttc gatgtgctcg aatacgcgca
     3001 tgaagttgcc gccgaggatc ttctcgacgt cgccctcgga aacaccgcgc tcgagcaggc
     3061 gcgcagttag cagcggcaag ccggccgcgg tttgcaggtc tttcggcagc gctgggacgc
     3121 cgtcgaagtc ggagccgatg ccgacgtgat cgatgccggc tacgtctgcg gcgtgcagga
     3181 tgtgatcgac ggcgtcgtcg atcgtcgcct gcgggcttcc gagggtgttg aaccactcga
     3241 ggtagagatc ccagtcggtg gcgtcgtcgg gcatctcttc cgtgccgcct gcagactcgc
     3301 tcttcacacg cttctgcgaa ttcatgatca cctgcgctac ggcgtcgttc agaaacgcgg
     3361 agaagaaatt gatcatgcag acgccgccgt tcttcgccag gtcgcggagc atgtcatcgg
     3421 tcatgttgcg cggatggcgg cacagcgcgc ggcacgacga atgcgtggcc acgagcggaa
     3481 cgcgcgtcgt ctccagcacg gcgtagaagg ccttgtcgga gacgtgggag atgtcgacga
     3541 tcatgccgag gtcgttcatg gtgcggacga cgtcgcggcc gaaatcggtg agaccgttgt
     3601 ggcgcccgcc gtcacccgag gaatcgcacc agttgttcgt gttgacgtgg gtgagcgtca
     3661 tgtagcgcgc gccgaggcgg tagaaatcgc gcaggatgga gagcgagtcc tcgatggcgt
     3721 gtccgccttc gacaccgatc atgagcgcgg tcttgccgtc gggcttggcc tgacgaatgc
     3781 ccgccgcgtc gtcgcaaaac gtcagcacat ccggccgtct cttgacctcg gcgtgaatga
     3841 gcccgatgag ccggtatgcg aaacgcgcgg cgccgcgccc
//
LOCUS       DATLVW010000438         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_429792, whole genome shotgun sequence.
ACCESSION   DATLVW010000438 DATLVW010000000
VERSION     DATLVW010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429792"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VNN25_13280"
     CDS             complement(<1..965)
                     /locus_tag="VNN25_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92548.1"
                     /translation="MHKFAAVMLSVAFLIAALPAEASNFRVADQVYLPIAGRVSVFRT
                     DVFISNLSSDFVTVKVVLASGDTGTLTPNLPQISLKPFERREMTDFFLTVLGISNGVG
                     QVVFSACRAASNCSNADANGENVNYRDISVESRIYAVDGDGKTNGQLFNGYPWYSYVS
                     MLAAPTGLDKVFITGLRNTGAVGQVGTFRTNVGVTNSSQYSSTTLTLHLFDRNGAAIG
                     DRQITLGPLGQVQQEISILFPAFVRSSDSTGAWLTVEQTSVTPTVDAGNQPGCEDGCP
                     GFFAYASLLDNATNDPTTLEAQYFAHLSSDALKALYKSGGNPPVRR"
     gene            complement(1205..1792)
                     /locus_tag="VNN25_13285"
     CDS             complement(1205..1792)
                     /locus_tag="VNN25_13285"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXH92549.1"
                     /translation="MNWLIKNFLRGLIIVVPIALTLYLIYEAFTRIDRLLNLRTPGVG
                     FAITIVAIVVIGALASNFFVRKFLNLTETVFTRAPLVRLIYASIKDLLEAFVGDKKRF
                     DQPVTVTITEGVRTLGFITQDDLEFLGLPGQVAVYLPFSYSMAGTLLLVHRDRVERVA
                     IDSASIMALVVSGGVSRAVKMNGGSVAGDSPTAAG"
     gene            1983..2375
                     /locus_tag="VNN25_13290"
     CDS             1983..2375
                     /locus_tag="VNN25_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HXH92550.1"
                     /translation="MLDRQAIADLYDRHASRLYALALRITGDRDAAADALADAFDALG
                     RNDEVRDPETYLVRATRDHALARQTQKRPAAVVPTVATPRQLVEDAWYGMTVSDLATV
                     YKISEGDVRGMLCAGMTELRGQLAAGTK"
     gene            2372..2824
                     /locus_tag="VNN25_13295"
     CDS             2372..2824
                     /locus_tag="VNN25_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH92551.1"
                     /translation="MTETLSIEDLNLLDAVVASTIEPVDPPAALRARILAAARDTPQN
                     TRTVRENEGRWLRLVPGVTMKELSVDKNRGTATVLLSLAPGSHLAGHDHHGPEDSFVI
                     SGSCRIGSTYLNKGDFHHADGGTHHGEIVSDDGCVLLLVIDRTDYMAA"
     gene            complement(2871..3416)
                     /locus_tag="VNN25_13300"
     CDS             complement(2871..3416)
                     /locus_tag="VNN25_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH92552.1"
                     /translation="MAPHRARVCLIGPECSGKTTLAERLAVRFDVPWVPEFAREYAQR
                     VGRLLTFEDVVPIARGQMALEDAAAYDDLIILDTDLISTVVYSGHYYDQCPVWIGHEA
                     RARKADLYLLTDIDIPWAADEVRDSAATRAALHGEFSVTLAAFGARFVVIRGDWDARF
                     AAAERAIDHAKTLTRRREDAK"
     gene            complement(3386..3958)
                     /gene="pnuC"
                     /locus_tag="VNN25_13305"
     CDS             complement(3386..3958)
                     /gene="pnuC"
                     /locus_tag="VNN25_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HXH92553.1"
                     /translation="MTFTPLEIAAVIFTLANVWLAVKENIWTWPTGIASVLLYLVVFW
                     RSHLYLNAGLQLVYFALSIHGWYEWLHGGENKTELKITRASSKMWTILMSIGVLLTVG
                     FFWLLRATSHDASLPIWDAVTTAFSIVGQFMLNTKIVENWLIWAIVDVIYVFMFIDQK
                     LYLTAGLYAFFVVLCVKGLLDWRRTAHASV"
     gene            complement(3955..>4361)
                     /locus_tag="VNN25_13310"
     CDS             complement(3955..>4361)
                     /locus_tag="VNN25_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92554.1"
                     /translation="DAAGNITGSEPITYHDVNPVLTPTMIVNQSIEYLVSSSFTLGVT
                     GRHVGRSYLDNTNNGSFVAPAFTTVDANAAIRLSHDTTLRVQVNNVLNDKRVFPSGYS
                     YLFLTPEKTISGISYYYPQATRNATVMVDFRR"
BASE COUNT          915 a         1367 c         1300 g          779 t
ORIGIN      
        1 cgccgcactg gtggattgcc gccgctcttg tagagcgctt tcagtgcgtc gctgctcaga
       61 tgggcaaagt actgggcctc cagcgtcgtc ggatcgttcg tcgcgttatc gagcagcgat
      121 gcgtacgcga agaaacccgg acacccatct tcgcatcccg gctggttccc ggcatcgacc
      181 gtcggtgtta cggacgtttg ctccacggtc agccacgcac ccgtggaatc cgatgatcgt
      241 acgaacgcgg ggaacaggat gctgatctct tgttggactt gaccgagagg ccccagcgta
      301 atctggcgat cgccgatcgc tgcgccgttc cgatcgaaga gatgaagggt cagcgtcgtg
      361 ctcgagtact gcgaggaatt cgtcacgccg acgttcgttc ggaacgtacc aacctgtccc
      421 accgcaccgg tgttgcggag ccctgtaatg aagaccttgt caagtccggt gggcgcagca
      481 agcatcgaga catagctata ccagggatag ccgttgaaga gctgaccgtt ggtcttgcca
      541 tcgccgtcga cagcgtagat ccgcgactca accgagatgt cgcggtagtt gacgttctcg
      601 ccattcgcat ccgcattgga gcaattcgag gctgcccggc atgcgctgaa gaccacctgt
      661 ccaaccccgt tcgagatgcc cagcacggtc aggaaaaagt ccgtcatttc gcgccgttca
      721 aatggcttga gcgagatctg cggcaggttc ggggtcagcg tcccggtgtc tcccgaggcc
      781 aggacgacct tcacggtgac gaaatcggac gagagattgg aaatgaagac gtcggtccgg
      841 aacaccgaga ccctcccagc aatcggcagg tacacctgat ccgcgactcg gaaattgctt
      901 gcctcggcgg gcaacgcggc gatcaggaac gcgacactca gcatcacagc tgcgaatttg
      961 tgcattggct tctccctatg aatcgactga ccgtgcgacc tgtgcgaagc gtgccgggcg
     1021 ctcgacgaga cgctccattc gcaaccgctg gaccatacgc cagaagaatg actccatccg
     1081 cggaaactgc tgacctgcgt aggtttgcgc cttgaggtcg cacgccagcc accttcgaat
     1141 cacacgtgcc ggtatgatta ttcgaacggt ccgggtgcta ataagtgaac gcaacctgac
     1201 ttggtcagcc ggccgcggtc ggcgagtctc cagcgaccga tccgccattc atcttcaccg
     1261 cccgcgagac gcctcccgat acgaccaaag ccatgatact ggcgctgtcg atggccacgc
     1321 gctccacgcg atcgcgatgc accagcagca gcgtccccgc catgctgtag gagaacggca
     1381 ggtagaccgc tacctgcccg ggaaggccga ggaactcgag atcatcctgc gtgatgaagc
     1441 ccagcgtccg caccccttcc gtgatcgtca ccgtcaccgg ctgatcgaaa cgcttcttgt
     1501 cgccgacgaa cgcctccagc aggtctttga tcgatgcgta gatcagcctc acgagcggcg
     1561 cgcgcgtgaa cacggtttct gtgaggttga gaaacttgcg gacgaagaag ttcgacgcca
     1621 gcgcaccgat gacgacgatg gccacgatcg tgatcgcgaa acctactccc ggcgtccgca
     1681 ggttcagcag acggtcgatc cgtgtgaacg cctcgtagat cagatagagc gtcagagcga
     1741 tcggtacgac gatgatgagg ccacgaagaa agttcttgat gagccagttc atggggacat
     1801 ggtagttcgg gggtggggag tggggggtcg ggagtgggag cagaacctgc tttccgactc
     1861 ctcgactacc ggcaacttac tacctaccga aaatcgagaa cccgaaagcg tcccgattac
     1921 cccgctccca caccccactc cccactcccc accccccaca tcagctaaac tcaccacacc
     1981 ccatgctcga tcgccaggcc attgccgacc tgtacgaccg ccacgcctcg cgcctgtatg
     2041 cgcttgcgct gcgcatcacc ggcgatcgcg atgcagccgc cgatgcgctc gccgacgcct
     2101 tcgatgcgct ggggaggaat gacgaggtcc gcgatccgga gacgtatctc gtccgggcca
     2161 cccgggacca tgctctggcc cgacaaaccc aaaagcggcc ggcagccgta gtaccaacag
     2221 tggccactcc tcgccaactg gtcgaggacg cctggtacgg catgactgtg agcgatcttg
     2281 caaccgttta caaaatctcg gaaggggacg tccgcgggat gttgtgcgct ggaatgacgg
     2341 agttacgcgg gcagttagcg gcagggacga aatgacggaa acgctctcaa tcgaagatct
     2401 gaatctgctc gatgccgtgg tcgcctcaac gatcgagccg gtcgatccgc cggccgcact
     2461 gcgcgcccgt attctggctg ccgcgcgcga cacgccgcag aacacgcgca ccgttcgcga
     2521 gaacgaggga cgctggcttc gcctcgtgcc gggcgtgacg atgaaagagc tgtcggtgga
     2581 caagaaccgc ggaacggcca ccgttctgtt gtcgctcgct cccggctccc atctggccgg
     2641 acacgatcac cacggccccg aagactcctt cgtcatcagc ggaagctgcc gcatcggatc
     2701 gacgtatctc aacaagggcg acttccatca cgccgacggc ggcacgcacc acggcgagat
     2761 cgtaagcgac gacggttgcg tgcttctact cgtcatcgac cgcacggatt acatggcggc
     2821 gtaagccctt ctcacgcgaa gacgcgaagg cgcgaagaaa aagcagctga ctacttcgcg
     2881 tcttcgcgtc ttcgcgtgag cgtcttcgcg tgatcgatcg ccctctccgc cgccgcaaac
     2941 cgcgcatccc aatccccgcg gatgacgacg aaacgcgcgc cgaacgccgc gagcgtcacg
     3001 gaaaactcgc cgtgaagtgc cgcgcgcgta gccgcggagt cgcgcacctc atcggcagcc
     3061 cacgggatat cgatgtcggt cagaagatag agatccgctt tgcgcgcgcg tgcttcatgc
     3121 ccgatccaca ccggacactg atcgtagtag tgaccgctat agacgaccgt gctgatcaga
     3181 tccgtgtcga ggatgatcag atcgtcgtat gcggccgcgt cttcgagcgc catctgcccg
     3241 cgggcaatgg gaacgacatc ctcgaaggtc agcagccggc caacacgctg cgcgtactca
     3301 cgcgcgaact cgggaaccca cgggacatcg aacctgaccg caagccgctc tgccagcgtc
     3361 gtcttacccg agcattccgg gccgatcaga cagacgcgtg cgcggtgcgg cgccaatcga
     3421 gaagcccttt cacgcaaagt acgacgaaga aggcgtaaag cccggctgtg agatacagct
     3481 tctgatcgat gaacatgaag acgtagatca cgtcgacgat cgcccagatc agccagttct
     3541 ccacgatctt cgtgttcagc atgaactgcc cgacgatgct gaacgcggtc gtcaccgcat
     3601 cccagatcgg cagcgaggcg tcatgcgacg ttgcgcgcag cagccagaag aatccgaccg
     3661 tgagcaggac gccgatcgac atcagaatcg tccacatttt cgacgatgcc cgcgtgatct
     3721 tcagctcggt cttgttctcg ccgccgtgca gccactcgta ccaaccgtga atcgaaagcg
     3781 caaagtagac gagctgcagg cccgcattca gatagagatg cgaccgccag aaaacgacga
     3841 ggtagagcag gacgctggcg atgccagtcg gccacgtcca gatgttctcc ttcacagcca
     3901 gccacacgtt cgccagcgtg aaaatgaccg cggcgatctc gagtggcgtg aaggtcaacg
     3961 ccggaaatct accatcaccg tggcgttccg cgtggcctgc gggtagtagt aggagatgcc
     4021 gctgatggtc ttctccgggg tgagaaagag atagctgtag ccgctcggga agacgcgctt
     4081 gtcgttgagg acgttgttga cctgcacgcg caaggtcgtg tcgtgcgaga ggcggatcgc
     4141 cgcgttcgca tcaacggtcg taaaagccgg cgcgacgaag gaaccgttgt tcgtgttgtc
     4201 gagataggag cgtccgacgt ggcgtccggt cacgcccagg gtaaatgacg acgacacaag
     4261 gtattcgatc gactgattga cgatcattgt cggcgtcagc acgggattga cgtcgtgata
     4321 cgtgatcggc tcactgccgg tgatgttccc cgcggcatcg t
//
LOCUS       DATLVW010000439        13571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_63315, whole genome shotgun sequence.
ACCESSION   DATLVW010000439 DATLVW010000000
VERSION     DATLVW010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63315"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /locus_tag="VNN25_13315"
     CDS             <1..2234
                     /locus_tag="VNN25_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH92555.1"
                     /translation="PANIMLTKSGAKLLDFGLAKESPLGGAETDATVAKALTSEGTIV
                     GTFQYMAPEQLEGANADARTDIFSFGAVLYEMATGRRAFEGKSRASLIASILGTQPPP
                     IAQVRPMTPPALDRLVQACLAKDPDDRWQTAHDLMLELRFITESSSAAGVPAPVARRR
                     AWRERAAWIVAAAAVVAATVIAITAMKRTPRAGDLVQFAVEIPPNLALFPFDTKGVAI
                     SPDGTMIAFVAGDVQGKQSLYIRNLGTTKITALAGTDDASYPFWSPDSSQLGFFAGRK
                     LHRIDAKGGTTVTLCDAASGRGGTWNRDGVIVFAPTISSELYRISAGGGRPERVTSFE
                     RKQVRHRWPWFLPDGNRFLYVAGEDLVAASLDGKLHKQIVTNVSNAVFVTPDRLVFSR
                     GTVLMSQRFDPDALAVSGEPVPLPFGNVAYMSSKQLSIVSASENGTLAFLPAPEGATR
                     LVWVDAKGREDGEIGEAGGYDDAALSPDGKRVAVVRSAPDGNDIWLVDASNGSLSRFT
                     FHPGQYGFPCWSRDSKQIAFFLQMDAVGQVCIKSLDGTERTPVLRSQAWQIPNDYSPD
                     GTTLLTFVQTPTAAGDLYTLSLGEKPVMTPFVATPFDESAATFSPDGRWVAYHSNASM
                     RNEVYVRRYPLSPEQWQISNAGGESPMWSPDGKELFYASGDTIMRVPIGGGASLNAGT
                     PAPLFHIPGHHTAPRLTGSVSRPVISGITPDKQHFLFRLSTEQGLPSINVVLNWRKAL
                     QER"
     gene            complement(2469..3014)
                     /locus_tag="VNN25_13320"
     CDS             complement(2469..3014)
                     /locus_tag="VNN25_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92556.1"
                     /translation="MKYVSVLLAIILAVFPASAFANCPTQTGQYYSGPFSWYEFSFDT
                     SCASTTGNVSAATLSCFSLPSYQWDYGSGTVDYQMTVPSNVFSNFSVAVFVDFSDPHS
                     YSGDAISATVTAMHNSTVTHSETFFIHQGNQGSLSCTRFDSSSFTASAGDTIEVVMTG
                     QSYYSDATMQISTPVVFSSSI"
     gene            complement(3209..5539)
                     /locus_tag="VNN25_13325"
     CDS             complement(3209..5539)
                     /locus_tag="VNN25_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH92557.1"
                     /translation="MSEGQGPRRVGALLVALMVAVAILGPLLSPYDPRAWTAAPFQPP
                     SLHHWLGTDDMGQDLLSIWLYGARHSVAIALLAAIGATLLGTLVGAGAGYRRGRWDFA
                     AMRLVDFFLTLPTLPLVLVVAFYAGASRPLLVAVLIFSSWARCARELRPQAAALRDAD
                     FVVAERAMGATETSILIRHIIPVLWPMILAQFARLAHQAVLMESGLSFLGLGDSQWVS
                     WGGTLYYANARAVFLGNSWLWWGLPPGLGIGLLITGFALLGVGRPRGAALRAAMIPSG
                     PCPDPDSAHLFQIRDLEVGYSGRTILQGVDLHLDEGEVLGLVGVSGAGKTTLVQTVLG
                     LLPHAEIHRGGVWLAGRELLRMPERERRLQRGKEIAWISQAAMQSLNPVRNIGSQLRE
                     VAGMDGDRQSVEARISSALTSVGLSHDLKKAYPHELSGGMRQRVVIAMVLCRKPRVLL
                     ADEATSGLDSDRAREILDLLTRLCRERKMALLLISHDLALLERHCDRLAILDRGQIVE
                     SGPPFALLAAPSSAAGKALAEAACPRPRSPIHRPTDPAIVLDLSAVDFTYSTGAGLKN
                     VSLQVHAGESVGLVGPSGAGKTTVARLALRLLRPGRGSVQLMGRDLAALHGRALRDHL
                     RQAHLIFQDPFSALPWHQRVKTIVAEPLRLAGVDRDTRATAVISALAEAGLTPPENYA
                     DRNVDTLSGGERQRVALARALIAHPHLIIADEPTSMLDAPVKWVWLERLDALRRAQAL
                     GVLLITHDRAQANAFCDRIVTLDNGRLSGAGANRGG"
     gene            complement(5536..6504)
                     /locus_tag="VNN25_13330"
     CDS             complement(5536..6504)
                     /locus_tag="VNN25_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92558.1"
                     /translation="MTRTSRIAGYIVVLWLGATANFLLPRLLPGDPVDFLIGDDAGRL
                     TGAQRTAMLAQFGMDRPLIEQYRHYMSSIARLDLGTSLAHGKPVLKVIAARLPWTLLL
                     VGISIILAFAIGFILACFFHWVERARTSSALMCTVVFFGSLPPFWVGMVAIAVFAVAL
                     GWLPTHGAASLEEGITWGSVLRHALLPVATLTLGYIPSVFLVARASLEDALGEGYVAL
                     ARSHGATPLRVLLRQAAPIALRPLVNQFAMSFGVLLGGTVIVETVFAYPGLGLLLYEG
                     ILALDFPLVQSVFLLLVFSVVLANIAADILQVRLDPRLRSGLDATT"
     gene            complement(6501..8084)
                     /locus_tag="VNN25_13335"
     CDS             complement(6501..8084)
                     /locus_tag="VNN25_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH92559.1"
                     /translation="MRSRLVAAGIGLFACLALPGFAAPLRVAVAYEVTNLTPYTPGLP
                     ETLLELVYDKLAAPSPYLGNAQPWLATAIVPEGQDGKVWRIELRDGVHWHDGKPFTAS
                     DVVFTLRYYRDGTPNRWTHHVSDTPKLTTIEPIDRLSLRIGCERPCPMFDRVTAADLV
                     ILPEHLWRSVKEPHLYHGPLIGTGPYRVSQLEAGRFLRLEANRDYFGGVPRVASIFIS
                     FIRNPATAFAALCAGELDLVAAPVPPELVEPLSHRKGLAMTAGGNSPVFGVELRVNFD
                     RAPFSDPEFRRALALAIRPGEVLQRVALGQGVVGPFPALASPWTKPGLRQPSDDPRAA
                     AAIFDAMGFRDRNGDGLREDTHGAPLRFSLKVSSSEPLHQRAAQVIARQLKAVGFDIE
                     VDVVDPARSRALYTTRQFDLLIDDVTPHNLADPDQLMQSVMYGYLWHEGRSYPELDAL
                     ISQWRIASTVEARTEAGFELQELHSKAPVAIMLYYPKSHYAYRPQAYDGWRPIPGLGV
                     FHKWSLLQFAGGVPAWAKP"
     gene            complement(8091..10277)
                     /locus_tag="VNN25_13340"
     CDS             complement(8091..10277)
                     /locus_tag="VNN25_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH92560.1"
                     /translation="MSHVTRAQLLTLFVAVISAGIALGQTPPPSGNEKPPAADAPKEA
                     KPDERTEKKAVAPVVEQITVTGERFERPVDATPQSLTVMDSKDLHARPLSNVQTMLDD
                     APGISLQRSGGLDGQIVVRGLSSNDARIVLFIDGDRFRGRNFLEYSLLDPDEIERIEI
                     IRGPAAALYGSDAMNGLVNVITRRAAGDPAQQHFSLTPRLYALGYSSTNNMGAARLEL
                     QGIGDGFDMLLSGNYRKADNFHTPDGEVLNSDFAARGFNARVGYSPTPTRRFELIGKI
                     FSDDAGRANAPGAPLVITREDPLRERYVRAGFTESQAAPWLQDIDASLYVRVLSSILR
                     SVNHSASNGDVDTRNTWVIGPTETGGKLLARSIIGNNVLSYGFDFYNEDAPSFEDDDR
                     IVNKAGVTTFLDPRAKRVRDASQTDIGGLAHYDYDPTSQWTVSLGGRYDFVETKLSST
                     PAPGESPELSAAFARNLTARDTALTGSGGLIFRPLPTFHLVGNISTAFRAPTTSDKSG
                     SGIVGALNSLPNADLKPESSVNYELGARLRLQAVNLNLSTFRSDYKDLIQFQFLNPTT
                     RVAVNFGKSKVEGWELDGTYQMSRDMAWRFNAADVRATNTITGVPLSYVPPLNGLIGL
                     RRTFPVASSWLELTARWSNDKTRINPTQERPTDGYEVFSLYGGLDLGRYQPWLKSYRL
                     TAGIDNLTNKAYRSPVTKESLGFPRSFTNPVLEPGRSLSINLTAGF"
     gene            complement(10345..11235)
                     /locus_tag="VNN25_13345"
     CDS             complement(10345..11235)
                     /locus_tag="VNN25_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92561.1"
                     /translation="MSANNSSEQTSVLQTITSPTLDPVDPLFQQYPSMKLGVRESVRF
                     YANLLVPLAERIIAGEPEVTEWVITAPPLYVVPAGANLLAHEMFPILRERVPRSRALR
                     LVDLRYALPMAQSNAHLLGEDYSSSDIAARIRNRQRLHEGDGAPRPDPADFAGRAVLF
                     INDINVTGTQQLYVQRTLDSVHPASIHWMYIFQVDPDLGRSYPGIEYALNFLNLGTFE
                     EFAEVIARAHVDYTSRCVSRLFSYPIEALEPLFHSLDRTRRERLFELATREGVYTEMA
                     DLAKLALLRESSSGNMANAH"
     gene            complement(11315..11605)
                     /locus_tag="VNN25_13350"
     CDS             complement(11315..11605)
                     /locus_tag="VNN25_13350"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HXH92562.1"
                     /translation="MKISVQRSGGFANIGSHAEVDTSTLPREKADVLQRLIRGAAFPD
                     QPAQPHRTAGAADVYQYDITVDDGTGSKTYRADELSIPEKWRAVVEHVLRQS"
     gene            11720..12016
                     /locus_tag="VNN25_13355"
     CDS             11720..12016
                     /locus_tag="VNN25_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXH92563.1"
                     /translation="MDEQEQPTIRITLDDSVAQGEYINFANIIHSPSEFVIDLGRIVP
                     GRTDVKVYSRAIMTPLHAKQLLEALAHNISLFEQKFGDIRIEGTTPFPTNEPSN"
     gene            12076..13398
                     /gene="trmFO"
                     /locus_tag="VNN25_13360"
     CDS             12076..13398
                     /gene="trmFO"
                     /locus_tag="VNN25_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXH92564.1"
                     /translation="MARVKIIGAGLAGCECAFGLAQRGHKVDLVEMRPSTTTAAHQTS
                     NLAELVCSNSFRSSAITNAVGLLKAEMALLESLVIRCGTDARVPAGDAFAVDRERFAA
                     AVTSAIASEPNITLRREEVTSLDRDGYDFLVVATGPLTSQPLFEAINNFLGRDGLYFY
                     DAIAPILAADSLDLTKLYWKSRYGKGSATDYLNAPMTQAEYEAFVAALLSAEIFAPHD
                     FEKAAHFEGCLPVEEIASRGIETLRFGPMKPVGLEHPVTGERPHAVVQLRKEDLSDDY
                     FNIVGFQTKMKVPEQKRVIQMIPGLENAVFARFGSMHRNTFINGPKHLRPTFQTKLDD
                     RVFFAGQITGVEGYVESSAVGLLVARYIDELSRDVAATPLPYFTALGALGRHVAESSP
                     DRYQPSNVTWAMIEDVIGRCRKIEKRDRQVEKAMTAIRAAAGASSNAA"
BASE COUNT         2622 a         4206 c         4316 g         2427 t
ORIGIN      
        1 agcccgccaa catcatgctg acgaagtcgg gagcgaaact gctcgacttc gggctggcca
       61 aagagagccc gctcggcggc gcggagaccg acgccaccgt tgccaaggcc ctcacctccg
      121 aaggaacgat cgtcgggaca tttcagtaca tggcgccgga gcagctcgaa ggcgccaacg
      181 ccgacgcgcg cacggacatc ttctctttcg gcgccgtgct ctatgaaatg gccaccggcc
      241 gccgtgcctt cgaagggaaa agccgcgcca gcttgatcgc ctccatcctc ggcacgcagc
      301 cgccgccgat cgcacaggtt cgtcccatga caccgccagc gctcgaccgc ttggtgcaag
      361 cctgcctggc caaggatccc gacgatcgat ggcagaccgc gcacgacctc atgctcgagc
      421 tgcgcttcat caccgagagc agctccgcgg cgggcgtgcc ggcccccgtc gcccgccgcc
      481 gcgcctggcg cgagcgcgcc gcgtggatcg tcgccgccgc tgccgtcgtc gcagcgactg
      541 tcatcgcgat caccgcgatg aagcgcacgc cgcgcgcagg cgacctcgtg cagttcgccg
      601 tcgagatacc gccgaatctg gcgctgttcc ccttcgacac caaaggagtg gccatctcgc
      661 ccgacgggac gatgatcgcg ttcgtggcgg gcgacgttca gggaaagcag tcgctgtaca
      721 tccgcaacct gggcacgacg aaaatcacgg cgctcgccgg caccgacgac gcgtcgtatc
      781 cgttctggtc gcccgatagc agccagctcg gcttcttcgc cggccgaaaa ctgcaccgca
      841 tcgacgccaa aggcggcacg accgtaacgc tctgcgacgc cgcatccggc cgcggcggca
      901 catggaatcg cgacggtgtg atcgtcttcg cgccgacgat ctcgagcgag ctgtatcgca
      961 tctccgccgg cggcggccgt cctgagcgcg tcacttcgtt tgaacggaaa caggtccggc
     1021 accgatggcc gtggtttctc ccggacggca atcgctttct ctacgttgcc ggcgaagacc
     1081 tcgtggctgc ctcgctcgac ggcaagctgc acaagcagat cgtcaccaac gtctccaacg
     1141 ccgtcttcgt cacgcccgac cgcctcgtct tctcgcgcgg cactgtgctg atgtcgcagc
     1201 gattcgatcc cgacgccctc gcggtcagcg gcgagcccgt cccgctcccc ttcggcaacg
     1261 tcgcctacat gagctcgaag cagctcagca ttgtgagcgc atcggagaac ggcacgctcg
     1321 cctttctccc tgcgcccgaa ggcgccacgc gtctggtgtg ggtcgacgcc aaagggcgcg
     1381 aggacggcga gatcggcgag gctggcggct acgacgacgc tgcgctctca cccgatggaa
     1441 agcgcgtagc cgtagtgcgc agcgcgcccg acggcaacga catctggctc gtcgatgctt
     1501 ccaatggcag cctgtcgcgc ttcacgttcc atcccggcca gtacggcttc ccgtgctgga
     1561 gccgcgattc gaagcagatc gccttcttcc ttcaaatgga tgccgtcggc caggtctgca
     1621 tcaagtcgct tgacggcacc gagcgcactc ccgtattgag gtcccaagcg tggcaaatcc
     1681 cgaacgatta ttcgcccgac ggcaccacgc tcctgacctt cgtgcagacg ccgacggcgg
     1741 caggcgatct ctacacgctg agcctcggcg agaaaccggt gatgacgccc ttcgtggcca
     1801 cgccgttcga cgaaagcgcg gcgacgttct ctcccgacgg ccgatgggtg gcctatcact
     1861 cgaacgcatc gatgcgcaac gaggtctacg tccgccgcta tcccctcagc cccgagcagt
     1921 ggcaaatttc gaacgcggga ggcgaaagcc cgatgtggag cccggacggg aaagagctgt
     1981 tctacgcctc gggcgacacc atcatgcgcg tcccgatcgg cggcggagcg tcgctcaacg
     2041 ccggcactcc cgcgccgctc ttccatattc cgggacatca caccgcgccg cgcctgaccg
     2101 gatcggtgtc gcggcccgtg atcagcggca tcacgcccga caagcaacac ttcctcttcc
     2161 gtctcagcac ggaacaaggg ctgccgtcga tcaatgtcgt gctgaactgg cggaaggcgc
     2221 ttcaggagcg gtagcccgcg agatgcggcc atcggtccag aaatgccgcc agcgcgcgtc
     2281 gctcaccgtc tatccgggtc taatccgtgc gttttcgcac atctgcggac aagtcgagac
     2341 cgggtaaacc gctggcgaaa ggagatgtcc gcgcatgtcc ggagaccgac gcggagagga
     2401 ggttcagttg gccggggttc aaatcccgca gcccgaccaa cacgaacccc atccctttga
     2461 cgatttcgtt agatgctcga cgagaagaca accggcgtcg agatctgcat cgtcgcgtcg
     2521 gagtagtagc tctggcccgt catcacgact tcgatcgtgt cgccagcgga agcggtgaac
     2581 gagctgctgt cgaaacgcgt gcagctcagc gagccttggt ttccctgatg gatgaagaac
     2641 gtctcggagt gcgtaaccgt gctgttgtgc atggcggtaa ccgtcgcgct gatggcgtct
     2701 ccggagtagc tatgcggatc gctgaagtcg acgaacaccg ccactgagaa gttgctgaag
     2761 acgttgctcg ggacggtcat ctgatagtcg actgtcccgc tgccgtagtc ccactgatac
     2821 gacggcaaac tgaagcacga caaggtggcg gccgagacgt ttcccgtcgt gctggcgcaa
     2881 ctcgtgtcga aactgaactc gtaccagcta aagggtcccg aatagtactg gcctgtctgc
     2941 gtggggcagt tagcgaacgc gctcgcagga aacaccgcta aaataatcgc cagcaacact
     3001 gacacatatt tcatttcagc ctccttacgg tatggttgag aatatatcat tcaggatgaa
     3061 ttgtcatagc gtctaaacat tcttgatgtc gctatgtcac gcggcagtcg cgacggtcga
     3121 cggaacacgt cgctcgttcc tcgctgatac ggatttcgag acgtcgatca gcggtgcttg
     3181 gagcgtgacc gcgttgccgg taagcagcct atccgccacg gttcgcgccg gcgccgctta
     3241 gccttccgtt gtccaacgta acgatgcggt cgcagaaggc gttggcttgg gcgcggtcgt
     3301 gggtgatcaa caggacgccg agggcttgtg ctcgccgcag agcgtcgagg cgttccagcc
     3361 agacccattt cactggggcg tcgagcatcg atgttggttc gtcggcgatg atcaggtggg
     3421 ggtgggcgat cagggcgcgg gccagggcta cacgctggcg ttcgccgcct gagagtgtgt
     3481 cgacgttgcg gtcggcgtag ttttcgggtg gggtgaggcc ggcttcggct agggcggaga
     3541 tgactgcggt tgcccgcgtg tcgcggtcga cgccggcgag gcgcagtggt tcggcgacga
     3601 tcgttttcac tcgctgatgc caggggagcg cggagaaggg gtcctgaaaa atgaggtggg
     3661 cctggcggag atgatcgcgc agcgcacggc cgtgcaacgc ggccaggtcg cgccccatca
     3721 attgcaccga tcctcggccc gggcgcagca accgcaacgc gaggcgcgct actgttgtct
     3781 ttcctgcacc gctcgggccg accagaccga ctgactcacc tgcgtgaacc tgtagcgata
     3841 cgtttttcag ccctgcaccg gtcgagtagg tgaagtccac cgcggagagg tcgagaacga
     3901 ttgccggatc ggtcgggcga tggatcgggc tgcgtggccg cggacacgcg gcttcggcga
     3961 gcgcctttcc ggcggcgctg ctgggagcgg ccaggagtgc gaaggggggg ccgctttcga
     4021 cgatttggcc gcgatcgaga atggccaggc ggtcgcagtg gcgctccagc agtgcgagat
     4081 cgtgcgagat cagaagaagt gccatctttc gctcgcggca gaggcgcgtg agcaaatcca
     4141 ggatctcgcg tgcgcgatcg ctgtcgagtc ccgacgtcgc ttcgtctgcc aaaagcacac
     4201 gcggctttcg gcagagcacc atcgcgatca ctacacgctg ccgcatcccg cctgagagct
     4261 cgtgcggata ggctttcttc aaatcgtgcg acaggccgac cgacgtcagc gcgcttgaga
     4321 tcctcgcttc gacagattgg cgatcgccgt ccatgcccgc gacttcgcga agctgcgagc
     4381 cgatgttgcg cacgggattg agcgactgca tcgccgcctg cgagatccat gcgatctctt
     4441 tgccccgttg gaggcggcgc tcgcgctccg gcatccgcaa caactcccgt ccggccagcc
     4501 acacgccgcc gcggtgaatc tcggcgtgtg gtagcaaacc gagaaccgtt tgaacgagcg
     4561 tcgttttacc ggctcccgac acaccaacga gcccgaggac ttcgccttcg tcgagatgca
     4621 agtcgacgcc ttgcaggata gtccgtcccg aatagccgac ttcgagatcg cggatctgaa
     4681 acagatgtgc ggaatcaggg tcggggcaag gtccgcttgg aatcatcgcc gcccgcaatg
     4741 ccgcgcccct cggcctgccg actccgagca gcgcgaagcc ggtgatcaac aatccgatgc
     4801 cgaggccggg cgggagcccc caccaaagcc atgaattgcc gagaaaaacg gcgcgggcgt
     4861 tggcgtagta cagcgtgccc ccccagctca cccattgtga gtcgccgagc ccgaggaatg
     4921 acagaccgct ttccatgagc acggcctgat gcgcgagccg ggcaaattgc gccaggatca
     4981 tcggccagag gacaggaatg atgtggcgga tcaggatgct ggtctcggta gcgcccatgg
     5041 cgcgctccgc cacgacgaaa tcggcgtcgc gcaaggccgc tgcctgcggc cgaagctcgc
     5101 gcgcgcaacg cgcccacgag ctgaagatca acaccgcgac gagcaatggc cggctcgcgc
     5161 ctgcgtagaa cgcaactaca agaaccagcg gcaacgttgg cagcgtcagg aagaaatcga
     5221 cgagccgcat ggcggcaaaa tcccatcgtc ctctgcgata gccggccccg gctccaacca
     5281 aagtgccgag caacgtcgcg ccgattgcag ccagtagcgc aatggcaacc gagtgcctcg
     5341 cgccgtagag ccagatgctc aacagatcct ggcccatgtc gtcggtgccg agccagtgat
     5401 gaagcgacgg cggctggaac ggagcggcgg tccacgcccg tggatcataa ggagagagaa
     5461 gagggccgag gatggccacc gcgaccatca acgcgacaag cagagcgcct acgcgccgcg
     5521 gaccttgtcc ctcgctcatg tagtcgcgtc cagtccgctg cgcaaccggg gatcgagccg
     5581 aacctgcaga atgtccgctg cgatgttcgc cagcactacc gaaaagacga gaaggagaaa
     5641 aacgctctgg acgagcggga agtcgagcgc gaggatgcct tcataaagca gcaagccgag
     5701 tccgggatag gcgaagaccg tctcgacgat gactgtgccg ccgaggagga cgccgaagct
     5761 catggcgaac tggttgacga gaggacggag ggcgattggc gctgcctgtc gcaacagtac
     5821 gcgcaaagga gttgcgccgt gagagcgcgc caatgcgacg taaccttcgc caagtgcatc
     5881 ctccagggat gcgcgcgcaa caagaaatac cgaggggatg tacccgagcg tcaaagtggc
     5941 aacgggtagc agagcgtgac gtaagacgct cccccatgtg attccttctt cgagcgacgc
     6001 agcgccatga gtgggcaacc atcccagagc cactgcaaag accgcgatcg ccaccattcc
     6061 aacccagaat ggcggcagtg atccgaagaa cacgacagtg cacatcaacg ccgaggatgt
     6121 acgtgcacgc tccacccagt gaaagaagca ggcgaggatg aagccaatcg caaaggccag
     6181 gatgatcgag attccgacaa ggagtagcgt ccacggcagc cttgctgcga tcaccttcaa
     6241 cacgggcttg ccgtgtgcga gcgaggttcc gaggtccaat cgcgcgatac tgctcatgta
     6301 gtggcggtat tgctcgatta gtggccggtc cattccgaat tgcgccaaca tggcggttcg
     6361 ctgcgcgccg gtgagccgcc cggcgtcatc accgatcaga aaatcgacag gatcacccgg
     6421 aagcaagcgt ggcaacagga aattcgccgt cgcgcccagc cagaggacaa cgatgtagcc
     6481 ggcaatccgt gacgtgcgcg tcatggtttg gcccacgcgg ggacgccgcc tgcgaactgg
     6541 agcagtgacc atttatgaaa cacgcccagt ccgggaatgg ggcgccaacc gtcgtacgct
     6601 tgcgggcggt aggcataatg ggatttcggg taatacaaca taattgcaac tggcgcctta
     6661 ctgtgcaatt cctgtaattc gaaacctgcc tcggttcgcg cttcaacagt cgatgcgata
     6721 cgccattggc tgatgagggc gtccagctcc gggtacgatc gtccttcgtg ccacaagtag
     6781 ccgtacatga cggattgcat gagttgatcg gggtcggcca gattgtgcgg cgtgacgtcg
     6841 tcgatcaaca agtcgaactg acgcgtggtg taaagtgccc gagagcgcgc cggatcgacg
     6901 acatcaacct ctatgtcgaa gccaacggcc ttcaactgcc gggcgatgac ctgcgcggcg
     6961 cgctgatgca gtggctcgct gctggaaacc ttcagactga accgcagcgg cgctccgtgg
     7021 gtatcttcac gcaatccgtc gccattacgg tcgcgaaatc ccatcgcatc gaaaatcgcc
     7081 gcggcagcgc gaggatcgtc actaggctgc cgcagaccgg gtttggtcca ggggctggca
     7141 agagctggga aaggcccgac gacaccttgc cccagcgcaa cccgctgcaa cacttccccc
     7201 ggccggattg cgagcgcgag cgctcggcga aactcaggat cggaaaacgg agcgcggtca
     7261 aagttgacgc gtagctccac gccgaagacc ggactgttcc ctccggcagt catagccagg
     7321 cctttacggt gcgacagagg ctcgaccagc tcgggcggca ccggcgcggc aaccaagtcc
     7381 agctcgcccg cgcacaatgc tgcaaatgca gtggctggat tacggatgaa cgagatgaaa
     7441 atgctggcca cacgcggaac gccgccgaaa tagtcgcgat tcgcttcgag gcgcagaaac
     7501 cgtcccgcct ccagttgcga cacgcgatac gggccggtgc cgatgagtgg cccgtgataa
     7561 aggtgaggct cttttaccga ccgccagaga tgttcgggga ggatgaccag gtcggcggca
     7621 gtgacgcggt cgaacatcgg gcaggggcgt tcgcagccga tgcgcagcga caaacggtcg
     7681 atcggctcga tcgtcgtcag cttcggcgtg tcgctgacgt gatgcgtcca gcgattcggc
     7741 gtgccgtcgc gatagtagcg cagtgtgaaa acgacgtcgc tggcggtaaa tggcttgcca
     7801 tcgtgccagt gcacaccgtc gcgcaactcg atgcgccaca ccttcccatc ctgcccctcg
     7861 ggcacaatcg ccgtcgcaag ccatggctgc gcgttgccga gatacggaga aggtgcggca
     7921 agcttgtcgt acacgagctc gagcaacgtc tcgggcagcc ccggcgtgta gggagtcaga
     7981 ttagtgactt cataggcgac cgctacacgc agcggcgccg cgaaacccgg taacgccagg
     8041 caggcgaaga ggcctatgcc ggcggcgacg aggcgcgaac gcaaacgacc ttaaaacccc
     8101 gccgtgaggt tgatgctcag ggagcggccg ggctcgagca ccggattggt gaagcttctt
     8161 gggaagccga gggactcttt ggtcacgggg ctacggtagg ccttattcgt gaggttgtcg
     8221 atgccggcgg ttagtcggta cgacttcaac cacggctgat aacggccgag atccaggcca
     8281 ccatagaggc tgaagacttc gtatccatcg gtgggacgtt cctgcgtcgg gttgatgcgc
     8341 gtcttgtcgt tcgaccatcg cgccgtgagc tcgagccaac tggaagcgac cgggaaggtg
     8401 cggcgcaatc caatgagccc gttcagcggt ggcacatagg agaggggtac gccggtaatg
     8461 gtattggtgg cacgcacgtc cgcggcgttg aaacgccagg ccatgtcccg cgacatctga
     8521 taggtgccgt cgagttccca cccctccact ttcgacttgc cgaaattcac ggcaacgcgc
     8581 gtggtcggat taaggaattg gaactggatc agatccttgt agtcgctccg aaacgtgctg
     8641 agattcagat tcacagcctg caaccgcaat cgcgcgccga gctcgtagtt cacgctcgac
     8701 tccggcttca ggtcggcatt cggcagcgag ttgagcgctc cgacgattcc ggagcctgat
     8761 ttgtcgctcg tggtcggtgc gcgaaacgcc gtactgatgt tgccgacgag gtggaacgtc
     8821 ggcagtggcc ggaagatcag gccaccgctg ccggtgagcg cggtgtctct cgccgtgagg
     8881 ttccgggcaa atgccgcgga gagctcgggt gattcgccgg gcgccggcgt ggagctgagc
     8941 tttgtctcga cgaagtcgta ccgcccgccg agcgagaccg tccattgcga cgtcggatcg
     9001 taatcgtagt gagccagccc cccaatatcg gtttgcgaag cgtcgcggac acgcttggcc
     9061 cgcggatcga ggaaggtcgt caccccggcc ttgttgacga ttcgatcatc gtcttcgaag
     9121 ctgggggcgt cctcgttgta gaagtcgaag ccgtaggaga ggacgttgtt gccgatgatc
     9181 gagcgggcca gtagcttgcc gccggtctcc gtcggtccga ttacccaggt gttccgggtg
     9241 tcgacgtcgc cattcgacgc tgagtgattg acgctgcgaa gaatgctgga aagtactctc
     9301 acgtagagcg aggcatcgat gtcctgcagc cacggggcgg cctggctttc ggtgaagccg
     9361 gcgcgaacgt accgctcgcg cagcggatct tcgcgcgtga tgacgagcgg agcgccggga
     9421 gcgttggcgc gtccggcatc gtcggagaag atcttgccga tgagctcgaa acgccgcgtg
     9481 ggagtgggcg aatagccgac tcgcgcgttg aaccctcgcg ccgcgaagtc gctattcagg
     9541 acctcgccgt cgggagtgtg aaaattgtca gccttccggt agttgccgga cagcagcatg
     9601 tcgaaaccat cgccgatgcc ctgcagctca agccgtgcgg cgcccatgtt gttggtgctg
     9661 gagtagccga gcgcgtagag ccgtggggta agcgagaagt gctgttgtgc cggatcgccc
     9721 gccgcgcgcc gcgtgatgac gttgaccagg ccattcatcg catccgaccc gtaaagcgct
     9781 gctgccgggc cgcggatgat ctcgatgcgc tcgatctcgt ccggatcgag caggctgtat
     9841 tcgaggaagt tgcggcctcg aaagcgatcg ccgtcgatga acaacacgat gcgcgcatcg
     9901 ttggagctga ggccgcgaac gacgatctgg ccatcgaggc cgccggaacg ctgaagcgag
     9961 attccgggcg cgtcgtcgag catggtctgc acgttggaca gcggccgtgc gtgaaggtct
    10021 ttcgaatcca tcacggtcag ggactgcggt gtggcgtcga cggggcgctc gaagcgctcg
    10081 ccggtcaccg tgatctgctc gaccacaggg gcgaccgcct ttttctcggt cctttcgtcg
    10141 ggtttggctt ctttcggagc gtccgcggcg ggaggcttct cgttcccgga gggcggcggc
    10201 gtctgcccga gggcgatgcc agcggagatc acggcgacga atagcgtcag cagttgggcg
    10261 cgagtgacgt gtgacataac aatgcctccg tgaagcttcg gaccggtcgc aagaatggcg
    10321 gactgcctga ttgacggcta tcgatcagtg ggcgttcgcc atgttaccgg aagaagactc
    10381 gcggaggagc gccagcttgg ctaaatctgc catttccgtg tacacgcctt ccctcgtcgc
    10441 cagctcaaaa agtctttcgc ggcgtgttcg atccagagaa tgaaaaagcg gctcgagcgc
    10501 ttcgatcgga tagctaaaga gccggctgac gcatcgagac gtgtagtcga cgtgcgcccg
    10561 cgcgatgacc tccgcgaatt cttcgaacgt gccgaggttc agaaagttca atgcgtactc
    10621 gattccgggg taggaacggc caagatcggg atcgacctgg aagatgtaca tccaatggat
    10681 cgaagcagga tgaacgctgt cgagtgtccg ctggacatac aactgctgcg ttcccgtgac
    10741 gttgatgtcg ttgatgaaaa ggacagcgcg gcctgcgaaa tccgccgggt ccggccgcgg
    10801 agctccgtct ccttcgtgca gccgctgccg gttcctgatc cgggccgcaa tgtccgagct
    10861 gctgtagtcc tctccgagga gatgggcatt gctttgtgcc atgggaagcg cgtatcgcag
    10921 gtcaaccagc cggagtgccc gggaacgagg aacgcgctcg cgcaagatcg gaaacatttc
    10981 gtgagcgagc agattcgctc cggcaggaac gacgtacagc ggcggcgcgg tgatcaccca
    11041 ctcggtgact tcgggctcgc ctgcgatgat cctctccgcg agaggaacga gcagattcgc
    11101 gtagaagcgc acgctctcgc gtacgcctag cttcatcgac gggtactgct ggaagagcgg
    11161 atcaacggga tcgagcgtgg ggctcgtgat ggtctgcaga acggaggtct gctcagacga
    11221 attgtttgcg ctcatcgatt gatgctttcg gacgaacgcg acgatacacc taccggtgta
    11281 attgttcagc tgcgagaacg tgatcggtgt cctctcagct ttgcctgagc acgtgctcga
    11341 ccacggcacg ccacttctcg ggtatcgaca gctcatcggc ccgatacgtt ttcgagccgg
    11401 tgccatcgtc tacggtgatg tcgtactgat agacatcggc agcgccggca gtgcgatgcg
    11461 gctgtgccgg ctgatcaggg aacgcggcgc ccctgatcag ccgctgcaac acgtccgctt
    11521 tctcacgcgg cagcgtggac gtatcgacct cggcgtgtga gccgatgttt gcgaatccgc
    11581 ccgagcgctg cacggagatc ttcatccctt cgatttcctc ggctgcggtt tgatgtacca
    11641 gatcctaccg gctttctccc cacatttctg ggacgagccc gggaatttcg actggatcgc
    11701 ggaggtatgc tcgcctgaaa tggacgagca agagcagccc accatccgca tcactctcga
    11761 tgacagcgtc gcgcaggggg agtacatcaa cttcgcgaac atcatccatt cgccgagcga
    11821 gttcgtgatc gacctcggac gcatcgttcc cggacggacg gacgtcaagg tctacagccg
    11881 cgcgatcatg acgccgctgc acgccaagca actcctggaa gcgctggcgc acaacatctc
    11941 gttgttcgag cagaagttcg gcgacattcg catcgaggga acgacgccgt ttccgacgaa
    12001 cgagccttcg aactgacgat gcagggccat cgtcgttcat gacgatggag tcgggacgaa
    12061 cgggaaacca ttcacgtggc acgagtcaaa atcatcggtg ccggtctagc cggctgcgag
    12121 tgcgcgttcg gccttgcaca gcgcggccac aaggtcgatc tcgtcgagat gcgtccttcg
    12181 acgaccaccg cggcgcacca gacgtcgaat ctcgccgagc tcgtttgctc gaactccttc
    12241 cgctcttccg cgatcacaaa cgccgtcgga ctgttgaagg cggagatggc gctgctcgaa
    12301 tcgctcgtca ttcgttgcgg cacggacgcc cgcgtgcctg ccggggacgc cttcgccgtc
    12361 gatcgggagc gcttcgccgc cgcagtgacg agcgccatcg cctccgagcc gaacatcacg
    12421 ctgcgccgtg aagaggtcac gtcgctcgac cgtgacggat acgactttct cgtcgtcgct
    12481 accggcccgc tcacctccca gccgctcttc gaagcgatca acaacttcct cgggcgcgac
    12541 ggtttgtatt tctacgacgc gatcgctccg atcctcgcgg ccgattcgct cgacctcacg
    12601 aagctctact ggaaatcgcg ctacgggaag ggctccgcca ccgactacct caacgcgccg
    12661 atgacgcagg cggagtacga agcgttcgtc gccgcgctgc tgtcggccga gatctttgct
    12721 ccgcatgact tcgaaaaagc cgcgcacttc gagggctgcc tgcctgtcga agagatcgca
    12781 tcgcgcggca tcgagacgct tcgcttcgga ccgatgaagc cggtcggcct cgagcacccg
    12841 gtgaccggcg aacggcctca cgcggtcgtc cagcttcgca aagaggatct ctcggacgac
    12901 tacttcaaca tcgtgggctt ccagacgaag atgaaggtgc ccgagcaaaa gcgcgtgatc
    12961 cagatgattc ccggcctcga gaacgccgtc tttgcgcgct tcggctcaat gcaccgcaac
    13021 acgttcatca acgggccgaa gcatctgcgg ccgacgtttc aaacgaaact cgatgaccgc
    13081 gtcttcttcg cggggcagat cacgggcgtt gaaggctatg tggagtcgtc cgcggtcggt
    13141 ctgctcgtgg cccgttatat cgatgagttg tcgcgcgacg ttgccgcaac gccgttgccc
    13201 tacttcaccg cgcttggtgc gctcgggcgc cacgttgccg aatcgtctcc cgaccgctac
    13261 cagccatcga atgtgacgtg ggcaatgatc gaggatgtca tcggccgctg ccggaaaatc
    13321 gagaagcgcg accggcaggt cgaaaaggcg atgacggcga ttcgggctgc agctggggcg
    13381 agttccaacg cggcgtaaat ctgcgggcta gcaaaccctt tgcaccatag agacacaaag
    13441 gtcacagagg ttttctctgt gtcctcagtg tctctgtggt gctttcagct actgctgcgc
    13501 aatcgtcggc gagacggtcg gacctgccgc ggtcacgatg ttcgcctgcg gcgccggctg
    13561 tgcctccgac t
//
LOCUS       DATLVW010000440         7200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_268598, whole genome shotgun sequence.
ACCESSION   DATLVW010000440 DATLVW010000000
VERSION     DATLVW010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268598"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1359)
                     /locus_tag="VNN25_13365"
     CDS             complement(151..1359)
                     /locus_tag="VNN25_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH92565.1"
                     /translation="MAEPRMAMELVQSSEFSEPAGDLAVMLTGGGARAAYQVGLIKGL
                     ARHFPNLRFQIITGVSAGAINAIFLAAHDGPLRAAADALGDLWLALESQHVFRPNYAA
                     LLPFRAAWKALLPPRMTKRPHGLLNAGPLAETLRHIYDTPVRNQPIAGIQRNLDHGDL
                     KAVALIGLDYTTGQNVRWTQGRCDPVFEGPNRRSVQTEITVEHVLASAALPFVFPAVK
                     IDSRWYGDGGIRLAAPLSSAVHLGATRILAMSTGYQRTAEEAATPVLHGYPPAAQIMG
                     QLINAIFLDVIDEDVNRMERMNEMIEKLDSSQRNGFKPVDLLVLRPSEDLGKIAGEHE
                     KYLPRNLKLITRAMGTRETESPDFISMLMFVPQYTSMLMEIGEADVASRLDELRTFLG
                     ETAGTAIRAV"
     gene            1407..2534
                     /locus_tag="VNN25_13370"
     CDS             1407..2534
                     /locus_tag="VNN25_13370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HXH92566.1"
                     /translation="MRVAVIGGGINGLGVAWELARRDYDVTLFEKGRCGAQTSSATTK
                     MIHGGLRYLEHAHIALVRESLRDRAWLLENLPELVKPVEILLPVYADSPRSSLTLRAG
                     LTLYDLLAGRSNIAPHMAMSAGELIERVPLIADGLRSGFSYWDAQVDDYALARVVVAS
                     AIREGAKVREETRVESLRRQESDWVVGTASGEEQRFDFVVNAVGPWMNALLAENRIHS
                     RYVLSLVRGSHLVLRNRVADVGMLLQSLSDRRVFFVLPWKGTTLVGTTEVMHRTSLEN
                     VLATGEEIDYLIARFNRYLREPITYDEVASTFAGVRPLVGRALNPSAIGRDYRLERRG
                     NLLNIFGGKMTTFRSMAEKVAMRVDNYFGHVRVARPARFVG"
     gene            2798..4603
                     /locus_tag="VNN25_13375"
     CDS             2798..4603
                     /locus_tag="VNN25_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXH92567.1"
                     /translation="MTATAPSAGRKSREVTITYLGEMLQNAGFIDEKQKIEVDNLDRQ
                     LRAQSRSARARGEEEASPFKALMGMNLTDASGSGTRIDDFLLARLIAEDARLKFFKID
                     PLKLDLEMIEAKISRPFAKKHRMTPVAVRDGRLIVAVVNPFDTVALDTYHQMVKQEIE
                     IVVSSESDVMGVITEQYGFRASVTKAGRDLGRGAIDLSNLEQYVKLKSGNEIETSDAH
                     IVNAVDFLLQHAYDSRASDIHIEPKRESAMIRFRIDGVLHEIQRVPKLVNLAITSRIK
                     TMCRMDIAERRRPQDGRIKTEREGKEVELRVSTLPTAFGEKLVMRIFDPDVLLRDLSG
                     LGFYEDELRMFADWIKRPHGIILVTGPTGSGKTVTLYSALKSLASPEINITTIEDPIE
                     MVWDEFNQTSVQTKIGITFPSALRTILRQDPDIIMVGEIRDEETAQNAVQAALTGHLV
                     FSSLHTNDSATSVTRLIDLGIQPFLISSTLVGTMAQRLMRRICEDCKRNRPLSVEEAG
                     MLNLQAPAGKRIIVKEGAGCIRCRNTGYFGRTGIFEILPIDNAIRDLIDRSEDFLKIK
                     DMAVKRGMRTLRQSALRKLAEGVTSFEEVVRVTGI"
     gene            complement(4613..4786)
                     /locus_tag="VNN25_13380"
     CDS             complement(4613..4786)
                     /locus_tag="VNN25_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92568.1"
                     /translation="MLTSLYEGKRALHAEQRALPYSEKIRQLLELQKIHYEIMRSRGV
                     ELRPWEKPWDIEL"
     gene            complement(4828..5289)
                     /locus_tag="VNN25_13385"
     CDS             complement(4828..5289)
                     /locus_tag="VNN25_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92569.1"
                     /translation="MNDLKASGVIDQYAIAGAMALMFWIEAISTYDLDVLVMLPQLDG
                     LLVSVDPIYRWAAERGYQAEHEHVVIEGFPVQFLPSFGALADEAIRTAADRQYHDVPL
                     RVVRPEYLIALYLVPSARTMKRRERAAALRESADIDRGLLDDLMRRFNLTF"
     gene            5437..>7200
                     /locus_tag="VNN25_13390"
     CDS             5437..>7200
                     /locus_tag="VNN25_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92570.1"
                     /translation="MVPALFLATSLFAQVSERIDVSAIEVPVVVRDAKGKVPAGLTPA
                     DFVLLEDGKPQQIIGVSYPVQAIATESAPSPVAPIAPTPARRWQVVIFIQQSLSTTRG
                     LSEALNDLASHAGELVALGDVQIVGDEGSAPHVIAPATGNAETLRSTLLDLAPKIRGQ
                     ELVTRLRADFLADEEADAAVRSPSTSQGNTIQSVRATASARFEAQIVRARQDAMLTWT
                     SRYQEPGRPHALLVVTTGYDLDPIDFYGRPDGERDTDLRGLGSAARQAEVAKAMAANG
                     WTVLSFAPGWMNNAGNPTFDVTNAGRGRLNDFVKSPKGGIVSPTGLNIRPLDPLRTLA
                     DETGGSVQTNGAKLGTDLDQLANRIVITYQLHRPRDGRAHHIEVKSLRPGVSVRAQRS
                     VISGTPEALAVARATLLAGDAGEERGELPVQCTVRQVASKNANDITSELVAFVTLTPI
                     DAVRSGLTAGTLRFSIAVRSAGAPPVTISKRMENLDLSKQANWRIDFQVHHEPGAAIG
                     VVAEEMATGAWGGVSCSGAETPATAADASVSLPPSGKWKTLPDAMAEAQAKGRLVLLD
                     LLPTVVDRNDLRRTRTKFRRQD"
BASE COUNT         1360 a         2213 c         2280 g         1347 t
ORIGIN      
        1 ctgtacgaga tccagtatcg ggagacggcg gcggtggttt aggtttcggc tgtctgcatg
       61 ggtgttagag gcgattcacg cggagacgcg gaggacacgg aggtgttttt ctccgcatct
      121 ccgcgcctcc gcgtgaaaca agtgaacccc tcacaccgcg cggatcgccg ttcccgccgt
      181 ctcgccgagg aacgtgcgga gctcgtccag tcgcgaggcc acgtcggcct cgccgatctc
      241 catcagcatc gaggtgtatt gcggcacgaa catcagcatg gaaatgaagt cgggcgactc
      301 cgtctcgcgt gttcccatgg cgcgcgtgat gagcttgagg ttgcggggaa ggtatttctc
      361 atgctccccg gcgatcttgc cgaggtcttc ggagggtcgc agcacgagga ggtcgaccgg
      421 tttgaagccg ttgcgctgcg atgaatcgag tttctcgatc atctcgttca tccgctccat
      481 gcggttgaca tcctcatcga tgacgtcgag gaagatggcg ttgatgagct gacccatgat
      541 ctgcgcggcg ggcgggtagc cgtgcagtac cggcgtggcg gcctcctcgg ccgttcgctg
      601 gtagccggta gacatggcca ggatgcgggt ggcaccgagg tggacggcgc tggaaagcgg
      661 agcggcgaga cggatgccgc cgtctccgta ccagcgcgag tcgatcttca cggcggggaa
      721 aacaaagggc agcgcggccg aagccagcac gtgctcgacg gtgatctcgg tctgcacgct
      781 gcggcgattc ggaccttcga agacgggatc acagcgtccc tgcgtccatc gaacgttctg
      841 gccggtcgtg taatcaagtc cgatcagtgc gaccgctttc aaatcgccgt ggtcgagatt
      901 gcgctggatt ccggcgattg gctgattacg caccggagtg tcgtagatgt ggcgcagcgt
      961 ctcggccagc ggcccggcgt tgagcaggcc gtgaggacgt ttcgtcatgc gtggtggcag
     1021 cagcgccttc caagccgctc ggaacggcag cagcgccgcg tagttcgggc ggaacacatg
     1081 ctgggactcc agtgcgagcc agagatcccc gagtgcgtcc gcagccgcgc gaagcggccc
     1141 atcgtgcgcc gctagaaaga tggcgttgat ggcgcccgcc gagacgccgg taatgatctg
     1201 gaagcgaagg ttggggaagt ggcgggccag tcctttgatc agtccgactt ggtacgcggc
     1261 tcgtgcgccg ccgccggtga gcatcacggc cagatcgccc gccggctcgg aaaactcgct
     1321 cgactgaacc agctccattg ccattcgtgg ctcagccatt tcaaatcgtg cgccaatttt
     1381 gggaacaaca ggtacgattc gataccgtgc gggttgcggt catcggcggc gggatcaacg
     1441 ggcttggcgt ggcgtgggag cttgcacgcc gcgattacga cgtcacgctt ttcgagaaag
     1501 gccgttgcgg cgcccagacc tcgtcggcga ccacgaagat gattcacggc ggcctgcgct
     1561 acctcgagca cgcccatatc gcgctcgtgc gtgagtcgtt gcgcgaccgc gcctggctgc
     1621 tggagaacct gccggagctc gtgaagccgg tcgagattct gctgcccgtg tatgccgaca
     1681 gtccgcggag ctcgctcacc cttcgagccg gtctgacgct gtacgacctt ctggccggcc
     1741 gttccaacat cgcgccgcac atggccatga gcgccggcga gttgatcgag cgcgtgcctc
     1801 tcatcgccga tggattgcga agtgggttca gctactggga tgcgcaggtc gatgattacg
     1861 cgctggcccg cgtcgtcgtg gcgtcggcga tccgcgaagg cgcgaaggtg cgcgaggaga
     1921 cgcgcgtcga gtcgttgcgg cgccaggaaa gcgactgggt cgtcgggacg gcgagcgggg
     1981 aggagcagag attcgacttt gtcgtcaacg cggtcggtcc gtggatgaat gcgctcctcg
     2041 ccgagaatcg catccattcg cgctatgtcc tctcgctcgt gcgcgggtcg cacctggtgt
     2101 tgaggaatcg cgtcgccgat gtcgggatgt tgttgcaatc gctcagcgac cggcgcgtct
     2161 tcttcgtcct accgtggaaa gggacgacgc tcgtcggcac gacggaagtg atgcatcgga
     2221 cgtcgctcga aaacgtgctg gcgacggggg aggagatcga ctatctcatc gcccggttca
     2281 atcggtatct tcgggagccg atcacgtacg acgaggtggc ttcgaccttc gccggcgtgc
     2341 gcccgctggt cggccgcgcc ctgaatccga gcgccatcgg ccgcgattac cgcctcgagc
     2401 gccgcggcaa tctgctcaac atcttcggcg gcaagatgac gaccttccgt tcgatggcgg
     2461 agaaggtggc gatgcgcgtg gataactact tcggccacgt gcgtgtcgcg cggccggcgc
     2521 gattcgtggg gtgagttgaa gtggtcgcca aagcaagggc aaccacagag acacagagaa
     2581 cacagagagt ctctgtgacc tctgtactac taaccagcgg tttctgcgcg cgacgcaaag
     2641 attttcacca aaactgattg gttgcagatt tggtgggttt catttggttg cggctatgcc
     2701 gcgctgtgcc tctgtggtga tcggtttcca aacccaaaac ccggaaccac acgccgctag
     2761 cggaattcac cctccatccc tcatcattca ctctcctatg accgccaccg ccccatccgc
     2821 tggccgcaag tcgcgcgagg tcacgatcac ctacctcggc gagatgctgc agaacgccgg
     2881 cttcatcgac gagaagcaga agatcgaagt ggataacctc gatcgccagc ttcgcgcgca
     2941 atcgcgttcc gcccgcgcgc gcggcgagga ggaggccagt ccgttcaagg cgttgatggg
     3001 catgaacctc accgatgcca gcgggagcgg cacgcgcatc gatgattttc ttctcgcccg
     3061 attgatcgcc gaggatgcgc ggctgaagtt cttcaagatc gatccactca agctcgacct
     3121 tgaaatgatc gaagcgaaga tctcgcgtcc gtttgcgaag aagcaccgca tgacaccggt
     3181 cgcggtccgc gacggcaggc tgatcgtcgc ggtcgtcaat cccttcgaca ccgtcgctct
     3241 cgacacctat caccagatgg tgaagcagga gatcgagatc gtggtgtcgt ccgagagcga
     3301 cgttatgggc gtgatcaccg agcagtacgg tttccgagcc tcggtgacga aagccggccg
     3361 cgacctcggc cgcggcgcca tcgatctctc caacctcgag cagtacgtca agctcaagtc
     3421 cggaaacgag atcgagacca gcgacgcgca catcgtcaac gctgtcgatt tcctgctgca
     3481 acacgcatac gactcgcgcg ccagcgacat ccacatcgag ccgaagcgcg agtcggccat
     3541 gattcgcttc cgcatcgacg gcgtgctgca cgagatccag cgcgtgccga agctcgtcaa
     3601 tctcgccatc acctcgcgca tcaagacgat gtgccggatg gacatcgccg agcgccgccg
     3661 cccgcaggat ggccgcatca agaccgagcg ggagggaaaa gaggtcgagc ttcgcgtttc
     3721 gacgttgccc accgcgttcg gcgagaagct ggtgatgcgc atcttcgatc ccgacgttct
     3781 cctgcgcgat ctttccggtc tcggtttcta tgaagacgag ctgaggatgt tcgcggactg
     3841 gatcaagcgt ccgcacggca tcattctcgt caccggtccg accggctcgg gcaaaacggt
     3901 gacgctctac tcggcgctca agtcgctggc atcgcccgag atcaacatca cgacgatcga
     3961 agatccgatc gagatggtgt gggacgagtt caatcagacg tcggtgcaga cgaagatcgg
     4021 tatcacgttc ccctctgcgc ttcgaacgat tctgcgccag gatcccgaca tcatcatggt
     4081 gggcgagatc cgcgacgaag agaccgcaca gaatgcggtg caggcggccc tgaccggcca
     4141 tctggtcttc tcctcgctgc acacgaacga ttcggcgacg tccgtgacgc gcctgatcga
     4201 cctcggcatc cagccattcc tcatctcatc gacgctcgtc ggaacgatgg cgcaacgctt
     4261 gatgcgccgc atctgcgaag actgcaagcg caaccggccg ctcagcgtcg aagaagcagg
     4321 gatgctcaac ctgcaggcgc cggccggcaa acggatcatc gtcaaagaag gcgccggttg
     4381 catccgctgc cgcaacaccg gctacttcgg ccgcaccgga atcttcgaga tcctcccgat
     4441 cgacaacgcc atccgcgatc tcatcgaccg ctcggaagac ttcctcaaga tcaaagacat
     4501 ggccgtcaaa cgcggcatgc gcaccctgcg gcaaagcgcg ctgcgcaaac tggccgaggg
     4561 agtgacgagc tttgaagaag tagtgcgggt gacggggatt tgagcgctca gactacagct
     4621 cgatgtccca cggcttttcc caaggccgca gctctacacc tcgggaccgc atgatctcgt
     4681 agtgaatctt ctgaagttcg agcagttggc ggatcttctc tgagtacggc agcgctcgct
     4741 gttcggcatg cagcgcgcgc ttgccttcgt acaggctagt aagcaattcg tcggtcggtc
     4801 ttggcgggga ctcagtcatt tgactaacta aaacgtcagg ttgaagcgtc gcatcaaatc
     4861 gtcgagcagg ccgcggtcaa tatctgctga ttcgcgaagt gcggcggcgc gctcccttcg
     4921 cttcatggta cgtgccgagg gtacaagata aagcgcgatg agatactccg gcctgaccac
     4981 gcgcaatggc acatcgtgat attgccgatc ggcagctgtg cggatcgctt cgtcggcgag
     5041 cgcgccgaat gatggaagga actgcacagg aaatccttcg atgacaacat gctcgtgctc
     5101 ggcttggtag cctcgctctg cggcccatcg gtagatcgga tcgactgaga ctagcaggcc
     5161 gtctaattgg ggcaacatga caaggacgtc gagatcgtaa gtggaaatcg cctcgatcca
     5221 gaacatcagt gccatggcgc cagcaatcgc gtactggtcg atgacgccgc tggctttcag
     5281 gtcgttcaca gcgagaagag tcttttcgaa cgacggcatc gtccgagtat acgactcgtt
     5341 cgtgcgcgga ttcgcgaccc ctcgcgattc cgccattccg ttatgatcgc cgcgctcgtt
     5401 gcagccagga gatcccgatt gaatccgcgc gcggcagtgg ttcccgctct tttccttgcc
     5461 acctcactat tcgcgcaagt cagcgaacgg atcgatgttt ctgcgatcga ggttcccgtc
     5521 gtcgttcgcg acgcgaaagg gaaggtcccc gccggactca cgccggcgga tttcgtgctg
     5581 ctcgaggacg gcaagccgca gcagatcatc ggcgtctcct atccagtgca agcaattgcg
     5641 actgaatccg cgccatctcc ggttgcgccg attgcgccca cgcccgcccg aagatggcag
     5701 gttgtcatct tcatccaaca atcgctttcg acgacgcgcg gcctgagtga ggcgctcaac
     5761 gatctggcct cgcacgctgg cgaactggtg gcgcttggtg acgtgcagat cgtcggcgac
     5821 gaagggtccg cgccgcacgt gatcgcgcca gccaccggta acgccgaaac gctgcgatcg
     5881 acgcttctcg atctggctcc gaagattcgt ggtcaggagc tggtcacgcg gttgcgcgcg
     5941 gactttctcg ctgacgaaga ggcagatgcc gcggtccgct cgccgtctac gtcgcaaggg
     6001 aacacgattc aatccgtgcg tgccacggcc agcgcgcggt tcgaggcgca gatcgttcgg
     6061 gcccgtcagg atgccatgct gacctggacg tcacgctacc aggagcccgg gcgtccccat
     6121 gcgctcctcg tcgtcacgac cggttacgac ctcgatccaa tcgatttcta cggcaggccg
     6181 gatggagagc gagacaccga ccttcgcgga ctcggctccg ctgcgcgcca ggcggaagtc
     6241 gccaaggcca tggcggcgaa cggctggacc gtcctcagct tcgcgccggg ctggatgaac
     6301 aacgctggaa atccaacctt cgatgtgacg aacgcggggc gaggccggct gaatgacttc
     6361 gtcaagtctc cgaagggcgg catcgtgtcg cccaccggac tgaacatccg tcctctcgat
     6421 ccgctccgca cgttggccga cgagaccggc ggaagcgtgc agacaaacgg agcgaagttg
     6481 ggcaccgatc tcgatcagct cgccaaccgc atcgtgatca cgtaccagct tcaccggccg
     6541 cgggacggac gcgcgcacca catcgaagtg aaatcgctgc ggccgggcgt gagtgtgcgt
     6601 gcacaacgtt cggtcatctc cggaactccc gaggcgctgg cggtggcgcg agcgacactg
     6661 cttgcgggcg atgcaggtga agagcgcgga gagttgccgg tgcaatgcac ggtgcgccaa
     6721 gtcgcctcga agaacgcgaa cgacatcacc tcggagctcg ttgcgttcgt cacgctcacg
     6781 ccgatcgacg cggtccgttc aggactcact gccggcacgt tgcgcttttc catcgccgtc
     6841 cgttctgccg gcgcgccgcc agtgaccatc tcgaagcgga tggaaaacct cgacctttcg
     6901 aagcaagcga actggcgcat cgattttcag gtgcatcacg agcccggtgc ggccatcgga
     6961 gtggtcgcgg aagagatggc caccggagcg tggggcggtg tgagctgcag cggtgcggag
     7021 actccagcca ccgccgccga tgccagtgtc tctcttcctc cgtcaggcaa atggaagacg
     7081 ctgcccgatg ctatggcgga agcgcaggcg aaagggcgtc tggtactgct cgatctttta
     7141 cctactgtcg tggaccggaa cgatctgagg cggacgcgca ctaagttccg aaggcaggac
//
LOCUS       DATLVW010000441         8132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_18362, whole genome shotgun sequence.
ACCESSION   DATLVW010000441 DATLVW010000000
VERSION     DATLVW010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18362"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VNN25_13395"
     CDS             <1..698
                     /locus_tag="VNN25_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXH92571.1"
                     /translation="SPVIAINTPTHRRVGTVGRAIPGVEIKIAEDGEILSRGPHIMKG
                     YWNNDDATRQAIDPDGWFHTGDIGEIDADGFLKITDRKKDIIINAYGKNIAPQPLEGL
                     LKSSPYVGTPVLIGDRRKYLVALIVPNFDKLERDAKAMGVHFDTRPELLANDKVLKIY
                     QGEIDRFNQNLDRQEKIRRFALLPRDFTIDADEITPSMKVKRKMIDKNYKEIIDGLFA
                     DEDVDEERDTRRV"
     gene            702..2855
                     /locus_tag="VNN25_13400"
     CDS             702..2855
                     /locus_tag="VNN25_13400"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXH92572.1"
                     /translation="MTSLAEATPRSASAFRIERDGDLGILWFDLPGEKINKFSSAVMT
                     EFAGVVGELERDRDIKRVIVASGKTSIFIAGADVTEFSKATSAEQAREYTRFGQQTIH
                     RFSKLPQVTVAAINGAALGGGCELALACDYRVMSDSPKAQIGLPETKLGIYPAWGGVT
                     RLPRLIGLPSALDIILNGKTLDGKRAKKIGLVDEVVPAAILLDIARQFAAKPKRRGRG
                     RTKFYIEGNPLARRVIFNKARKAVLSQTHGHYPAPLKAIEVMEYGMSAGIERGLAREV
                     DEVVPLIVGSTNGSTAGSDVAQNLIRLFFLMEDSKKERIAAAPTEVRDSGVLGAGIMG
                     GGIAQLIADKTDATVRMRDINWKAIAGGMKAAAKIWKKKVDRRRMTRVEMQRKLARIT
                     STIDWSGFERCDLVIEAVVENVEIKRQVLREFEAIAKPGAIFATNTSTIPITDIAAEA
                     AKPENVAGMHFFNPVDRMPLVEVIRGAKTSDVAMATVANSARKLGKTVVYCNDGPGFV
                     VNRILGPYMNEAGFLLEEGNTIESLDKAMVDFGMPMGPMALLDEVGIDVAAKVAKILS
                     AAFGARMEPSHVVDALYTDGRYGKKNGKGLYLYASGKRLGPDASVYKLLGIRSTHPAD
                     ATAVVERMIFAMVNEAAMILDEKIVASPAELDLAMIMGTGFPPFRGGLLRYADKLGLP
                     YILARLDDLAQKHGKRFLATVALRKLAERDGKFYR"
     gene            2929..4698
                     /locus_tag="VNN25_13405"
     CDS             2929..4698
                     /locus_tag="VNN25_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92573.1"
                     /translation="MDLTQPLPALTQRQRIAFWCIAIVCAATRFLAMARSLWDWDEAL
                     FCLGMRSFDITAHHPHPPGFPVYIALGRIVRTIIPSDFRALQAVNLIAGMLVFPAIFF
                     LARELRFRFVTAAVAAALCAFFPNVWFYGGGAFSDIPSLVLVVFAVAFLFRGCRGANA
                     YLIGAALLALAAGIRPQNFVVGLAPGLLATWYRARVAWRDVVFAALVGAITLGVAFGG
                     AIAATGGYKPYFEAAKEHGQYITRIDSFRSPARPALWRLFDRFFVKQYESLALSFLTS
                     IFVAISIAGALAARDRAIGWLFLTFGPFALLAWLMLDRYSINRFSIGYCPLFAILAAD
                     GIGRLTRRWPRVEAWIGAVLIASFFGWTLPALAAVRNTVAPSVLAVEAVRDHLDKRTQ
                     RLFVARDMDPFFQYLLPNQPYVHVLDKRAMPFALDGRQPWILAEADERDHDGFVFHRE
                     RDHLWNIARRHYFDVGLMPVRSSPQFLSGWYAPEQQSSVQQRSMSSRAAIRLPGSSGE
                     SLLRIQFRVPGSILVSHGMVTISLNGHVLDRFAAQPDDNDHEYHVTPAPNSAPNLLEL
                     SVDGALDSRNIGLSLTCLSFGPE"
     gene            complement(4760..5389)
                     /locus_tag="VNN25_13410"
     CDS             complement(4760..5389)
                     /locus_tag="VNN25_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HXH92574.1"
                     /translation="MKKLTLALLCVAMAFVSFACHKEDTYNSATDTSATSATDTYAST
                     TSTTATDTSATTASATSTASTLNSDDTTFVNKTAQGGMAEVALGQMASTKATNPDVKA
                     FADRMVTDHGKANDELKQLAQTKGVTLPSDVDQESKDASDKLSKASGKEFDKAYIDAM
                     VKGHEGVVKAFEKESKDAKDPDLKAWVTKTLPTIQDHLKMAKETKAKVK"
     gene            complement(5438..6412)
                     /locus_tag="VNN25_13415"
     CDS             complement(5438..6412)
                     /locus_tag="VNN25_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92575.1"
                     /translation="MAANYRRLLPLSLLLLLISLARPGFALDTESTGLLNHAWPDVAS
                     TRVSDIGRGIGVVFSPDLSVDDNCRFYQSLGFACFQAADWEKVFDDVHRYNVLYPERR
                     IFTLVLETHGTNGNGLKLQGSYDPNADRSYVSAGALQQRLEPEGIYYVIISACNSGRL
                     LRPTIYNQLDPNNGDKLFLPATKGIVNASPDFVASRSAVTIITPQSSHIETTLVGKVA
                     ELSPASRRAITESARTLGIVPPKEFAVSDMMVAMLTRDPQLKLDSGTYVDALSRDTAP
                     VDRSEQLFTKFVRFVNRVAAKQYPAKTAKKTVARKTAKKPSSKAPARH"
     gene            6419..6694
                     /locus_tag="VNN25_13420"
     CDS             6419..6694
                     /locus_tag="VNN25_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92576.1"
                     /translation="MSDQWVVVDSDVVDAFVEDVVVDVVVTRGRVSPPVVEIGLPPPV
                     SVAVVPVVEVALLVTTAELAGSRLQAAPIVNTKAAATILAIFIKASS"
     gene            6764..7183
                     /locus_tag="VNN25_13425"
     CDS             6764..7183
                     /locus_tag="VNN25_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH92577.1"
                     /translation="MAELIGRFSAQAFAIMRIVTGLMFAMHGSQKLLQFPPQSGAGGG
                     GGLPPLMMVSGVIELVCGLLIAIGFFTGIAAFLASGEMAVAYFMVHAHNAFWPIENKG
                     ELAVVYCFLWLFVSAHGAGIWSLDNAMRRRTPVVTTV"
     gene            complement(7170..7766)
                     /gene="ruvA"
                     /locus_tag="VNN25_13430"
     CDS             complement(7170..7766)
                     /gene="ruvA"
                     /locus_tag="VNN25_13430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXH92578.1"
                     /translation="MIGYLEGTLKQLDATRALVLAGGVGYEIHISLQTYYKLEGQKSV
                     ALEIFTHVREDALSLYGFSSGEEKFAFEKLISISGIGPTLAQKILSGIDPPDLAEAVA
                     RGDVRKLSSIPGVGKKTADRIVLELRDKFLLPESKREDLPSSARLSVDDDVHSALINL
                     GYRPKDAEGALDVARKQLGNEAELSALLKAALRQLTRS"
BASE COUNT         1469 a         2447 c         2528 g         1688 t
ORIGIN      
        1 cgtctcccgt catcgccatc aacacaccaa cgcatcgccg cgttggtacc gtcggccgcg
       61 ccattcctgg agtcgagatc aagatcgccg aggatgggga gattttgagt cggggaccgc
      121 acatcatgaa gggctactgg aacaacgatg acgccacacg gcaggccatc gatcccgacg
      181 gctggttcca cactggcgac atcggcgaga tcgacgccga cggattcctc aagatcaccg
      241 accggaagaa ggacatcatc atcaacgcct acggtaagaa catcgctccg cagccgttgg
      301 aggggcttct gaagagctca ccgtacgttg gaactccggt gctcatcggc gatcgacgca
      361 agtacctcgt ggcgctcatc gttccgaatt tcgataagct cgagcgcgac gccaaagcca
      421 tgggtgtgca ctttgatacg cgacccgaac tgttggccaa cgacaaggtg ctgaagatct
      481 atcaaggcga gatcgatcgc ttcaatcaga acctcgaccg ccaggagaag atccgccgtt
      541 tcgctctgct tccccgcgac ttcaccatcg atgccgacga gatcacgccg tcgatgaaag
      601 tgaagcggaa gatgatcgat aagaactaca aggagatcat cgacgggctt ttcgccgatg
      661 aagacgttga tgaagaacgc gatacgagac gagtttgatc gatgacatcc cttgctgaag
      721 caacgccacg cagtgcctcc gcgtttcgga ttgagcgcga tggagatctg ggcattctct
      781 ggttcgacct gcccggtgag aagatcaata agttctcgag cgctgtcatg accgagttcg
      841 ccggcgtggt cggcgaattg gagcgggatc gcgacatcaa gcgcgtcatc gtcgcgtcgg
      901 gcaaaacgtc gatcttcatc gccggcgccg atgtcaccga gttctcgaaa gcgacttctg
      961 ccgagcaggc gcgcgagtac acccgcttcg gccagcagac gattcaccga ttctcgaagc
     1021 ttcctcaggt cacggtagcc gccatcaatg gcgctgcgct gggcggcggc tgcgagctgg
     1081 ctctcgcttg tgattaccgc gtcatgtccg acagcccgaa ggcacagatc gggctcccgg
     1141 aaacgaagct cggcatttat ccggcatggg gcggtgtgac gcgtcttccg cggctcatcg
     1201 ggttgccgtc ggcgctcgac atcatcctca atggcaagac gctcgacggc aagagagcaa
     1261 agaagatcgg cctcgtcgac gaagtggttc ccgcggcgat tctgctcgac atcgcccgcc
     1321 agttcgctgc gaaaccaaag cgcagaggaa gaggccggac caagttctac atcgaaggga
     1381 atcccctggc gcggcgcgtc atcttcaaca aagcccgcaa ggccgttctg tcgcaaacgc
     1441 atggtcacta tccggcgccc ctcaaagcca tcgaggtgat ggagtacggc atgtccgccg
     1501 gcatcgagcg cggactggct cgcgaagtcg atgaggtcgt gcctctgatc gttggctcga
     1561 caaatggttc gacagctgga tcggacgttg cgcagaatct aatccgtctc ttcttcctca
     1621 tggaagactc gaagaaggaa cggatcgccg cggctccgac cgaagtccgc gattccggcg
     1681 tgctcggcgc aggcatcatg ggaggcggta tcgcccagct catcgccgac aagaccgacg
     1741 ctaccgtccg gatgcgcgac atcaactgga aagcgatcgc gggcgggatg aaggcggccg
     1801 cgaagatctg gaagaagaag gtcgatcgcc gccgcatgac gcgcgtggaa atgcagcgca
     1861 agctggcccg catcacgtcg acgatcgatt ggagtggctt cgagcgctgc gacctcgtca
     1921 tcgaagcggt cgttgagaac gtcgagatca agcggcaggt cctccgcgag tttgaggcaa
     1981 tcgccaaacc gggtgccatc ttcgccacaa acacatcgac gatcccgatc acggacattg
     2041 cggcggaggc tgcgaagccg gagaacgttg ccgggatgca ctttttcaat ccggtcgacc
     2101 gcatgccact ggtcgaggtc attcgcggcg cgaagacgtc ggacgtggcc atggccaccg
     2161 tcgccaactc cgctcgcaag ctcggcaaga ccgtcgtcta ctgcaacgac ggacccggct
     2221 tcgtcgtcaa tcgcatcctc ggcccctaca tgaacgaggc cggctttctc ctcgaagaag
     2281 gaaacacgat tgagtcgctc gacaaggcga tggtcgactt cggcatgccg atgggaccga
     2341 tggcgctgct ggatgaggtc ggaatcgacg tcgccgcaaa ggtcgcgaag atcctgagcg
     2401 cggcctttgg tgcgcgcatg gagccatcgc acgtcgtcga cgcgctttac acggacggac
     2461 ggtacggcaa gaaaaacggc aaaggcctct atctctatgc atccggcaag agactcggac
     2521 ccgacgcgtc ggtctacaag ctgctcggca tcaggagcac gcatcccgcc gatgcgaccg
     2581 cagtcgtcga gcgaatgatc ttcgcgatgg tcaacgaagc ggcgatgatt ctcgacgaga
     2641 agatcgtggc gtcgccggcc gagctcgatc tggccatgat catgggcacc ggcttcccgc
     2701 cgttccgagg aggcttgctc cgttacgcgg acaaactcgg gttgccgtac attctggcgc
     2761 gcctcgacga ccttgcccag aagcacggca agcgattcct ggccaccgtt gcgttgcgca
     2821 agctggccga gcgggatggg aagttctatc ggtgaagttc ggagccgcca aataggccaa
     2881 cctgacaacc agtcacccta ttcatgagat cctttcgccg ctccggcgat ggatctcacc
     2941 caaccacttc ccgcactgac gcagcgccag cgcatcgcgt tctggtgcat tgcgatcgtg
     3001 tgcgcggcga cgcggttcct ggcaatggcc cgttcgctct gggactggga cgaggcgctc
     3061 ttctgtctcg gcatgcggtc gttcgacatc acggcccatc atccgcaccc gcccggattt
     3121 ccggtctaca tcgcactcgg gcggatcgta agaacgatca ttccgagtga cttccgagcg
     3181 ctgcaagcag tgaatctcat cgccgggatg cttgtgtttc cggcgatctt ctttctggct
     3241 cgtgagttgc gatttcgctt cgtcacagcg gcggtcgcgg cggcgctctg cgccttcttc
     3301 cccaacgtct ggttctacgg cggcggagca ttcagcgaca ttccatcgct cgtgctggtc
     3361 gtcttcgcag tcgcatttct ctttcgagga tgccgtggtg ccaacgccta cctcatcggc
     3421 gccgcactgc tcgcgctggc ggctggcatc cgcccgcaga acttcgtagt cggactcgcg
     3481 cccgggttgc tggccacgtg gtatcgggcc cgggtggcct ggcgcgacgt cgtcttcgcg
     3541 gcgcttgtcg gcgccatcac gctgggagtg gccttcggag gtgcgatcgc cgcaacgggc
     3601 gggtacaagc cctacttcga ggcagcgaag gagcacggtc agtacatcac ccgcatcgac
     3661 tcgttccgga gcccggcgcg tccggcgctc tggcgcctct tcgaccgttt cttcgtcaag
     3721 caatacgagt cgctggcgct cagctttctc acgtcgatct tcgttgcgat ctccatcgcc
     3781 ggcgctctcg ccgcacggga tcgcgcgatt ggctggctgt ttcttacgtt cggtcctttc
     3841 gcgctgctcg cgtggctgat gctcgaccgc tatagcatca atcgattctc gatcggctac
     3901 tgcccgctct ttgcgattct cgcagccgac ggcatcggac gcctgacgcg gcgctggccg
     3961 agggttgagg cgtggattgg agcggtgctg atcgcgtcgt tctttgggtg gaccttacct
     4021 gcgctcgcgg ccgtgcgtaa caccgtcgct ccgtccgtgc tggcagtgga agcggtcagg
     4081 gatcacctcg acaaacgaac gcagcggctg ttcgtggcgc gcgacatgga cccgttcttc
     4141 cagtacctgc taccgaatca accgtacgtc cacgttctcg acaagcgggc aatgccgttt
     4201 gcgctcgatg gccgtcagcc gtggattctt gcggaggctg acgagcggga tcatgacggc
     4261 tttgtttttc atcgcgagcg cgatcatctc tggaacattg cgcgccgtca ctacttcgat
     4321 gttggattga tgcccgtgcg cagttcgccg caatttcttt ccggctggta tgcaccggaa
     4381 cagcaaagtt ccgtgcagca gcggtcgatg tcgtcgcgcg ctgcgatccg gcttcccgga
     4441 tcgagcgggg aatcgctctt gcggattcag tttcgtgttc ccggctcaat cctcgtgagc
     4501 cacgggatgg tgacgatctc cctcaacggt cacgttctcg atcgcttcgc cgcgcagccg
     4561 gacgacaacg accacgagta tcacgtcacg ccagcgccga atagcgcacc gaacctgctc
     4621 gagctttcgg tcgatggtgc gctcgactcc cgcaacatcg gcttgtcgtt gacgtgcctt
     4681 tcgttcggcc ccgagtgagc cacaaacgca aacgcgccgg ctcgcaccgg cgcgttcgtc
     4741 ctcagacacg agcgagtggt tacttcacct tcgccttggt ctctttcgcc atcttgaggt
     4801 gatcctggat cgtcggaagc gtcttggtta cccacgcttt caggtctggg tccttagcgt
     4861 cttttgattc tttttcgaac gccttcacaa ctccttcgtg gcctttgacc atggcatcga
     4921 tgtaggcctt atcgaactcc ttgccgctgg ccttggagag tttgtcggag gcgtctttgc
     4981 tctcttgatc gacgtcggaa ggcagcgtga cacccttggt ctgcgcgagc tgtttcagct
     5041 cgtcgtttgc cttgccgtgg tcggtaacca tgcggtcggc gaaggctttt acatcaggat
     5101 tggttgcttt cgtggatgcc atttgtccca aggccacttc ggccattccg ccttgggcag
     5161 tcttgttcac gaacgtggtg tcgtcgctgt tgagcgtgct ggccgtactc gtggcagacg
     5221 cggttgtcgc cgaagtatcc gtggccgtcg tcgatgtcgt tgaggcatag gtatctgtgg
     5281 cgctggtcgc cgaggtgtcg gtggcgctgt tgtaggtgtc ctctttgtgg caggcgaagc
     5341 tgacgaacgc catcgcgacg caaagcagtg caagagtcag tttcttcatc ggtcatctcc
     5401 tccgcgtagc gttttgtgca tgaagcgctc cagaaaacta atggcgagcg ggagctttgg
     5461 aggagggttt cttcgcggtc ttgcgcgcga cggttttctt cgccgttttc gccggatact
     5521 gcttcgcagc cacgcggtta acgaagcgga cgaacttggt gaatagctgc tcgcttcggt
     5581 cgaccggcgc cgtgtcgcgc gacaacgcat cgacgtacgt ccccgagtcg agcttgagct
     5641 gcggatcgcg ggtgagcatg gccaccatca tgtcggaaac ggcgaactcc ttcggcggaa
     5701 cgatgccgag ggttcgggca gactccgtga tggcgcggcg ggaggcgggg gagagctcgg
     5761 ccaccttgcc gacgagcgtc gtctcgatgt ggctcgactg cggcgtgatg atcgtgaccg
     5821 cgctgcgcga agccacgaag tcaggggagg cattgacgat ccctttcgtc gccggcagga
     5881 aaagcttgtc gccgttattg ggatcgagtt ggttgtagat cgtcggacgc agcagccggc
     5941 ccgaattaca cgcgctgatg atgacgtagt aaatcccctc gggctcgagg cgctgttgca
     6001 gcgcgccggc cgagacgtag gagcggtccg cattgggatc gtagcttcct tgcagcttga
     6061 gaccattgcc gttcgtgccg tgcgtctcca ggacgagcgt gaagatgcgg cgctcgggat
     6121 agagcacgtt gtagcgatgg acgtcgtcga agaccttttc ccagtcggct gcctggaagc
     6181 aggcgaatcc gagagactga tagaaccggc agttgtcgtc gacggaaaga tcggggctga
     6241 aaacgactcc gatccctcgt ccgatgtcgc tcacgcgagt cgaggccacg tccggccagg
     6301 catgattgag aagccccgtg ctttccgtgt cgagagcgaa cccggggcgg gcgagagaaa
     6361 tgagaagcag tagaagcgag agcgggagaa gtcgtcgata gttcgcagcc atggcgaagt
     6421 gagtgatcag tgggtcgtag tcgattcgga tgtcgttgat gcgtttgttg aggatgtagt
     6481 cgtcgatgtc gtcgtgacgc gcggccgggt gtctccgccc gtggtcgaga tcggcctccc
     6541 tccgcccgtc tcggtggcag tcgttccagt cgtggaggtg gcactgctgg tgacgacagc
     6601 cgagttggcc ggttcacggt tgcaagctgc accgatcgtc aacacgaaag cggcggcgac
     6661 gattctggcg atcttcataa aagcctcctc ttgacgtgac actgagcaat cgtcgcgccc
     6721 ggttggcccg gagcatgaac acatccagcg cggaggtgca gttatggctg agcttatcgg
     6781 acgattcagt gcacaggcct tcgcgatcat gcgcatcgtg accggcttga tgttcgcgat
     6841 gcacggatcg caaaaacttc tgcaatttcc gccgcaaagc ggggcgggcg gaggcggtgg
     6901 gttgccgccg ctcatgatgg tgtccggtgt catcgagctc gtctgcggtc tcttgatcgc
     6961 tatcggtttc ttcaccggca ttgccgcgtt tctggccagc ggcgagatgg ccgttgccta
     7021 cttcatggtt cacgcgcaca acgccttctg gccgattgaa aacaagggcg agctggcggt
     7081 cgtctactgt ttcctctggc tcttcgtctc cgcgcacggc gccggaatct ggagcctcga
     7141 caacgcgatg cgccgtcgca cgcccgtcgt cacgaccgtg tgagttgcct gagcgcagcc
     7201 ttgagcaacg cggacagctc ggcttcgttg ccgagctgtt tgcgtgcgac atccagcgcc
     7261 ccctcggcgt ccttcggtct gtagccgagg ttgatgaggg ctgagtggac atcgtcgtcg
     7321 acggaaaggc gcgccgacga tggcaaatcc tcacgtttcg attcggggag gagaaacttg
     7381 tcgcgcagct cgaggacgat gcggtcggcg gttttctttc ccacgccggg aatcgacgac
     7441 agcttcctca catcaccccg cgccaccgcc tcggcgagat caggaggatc gatgccggag
     7501 agaatcttct gcgccagcgt cggaccgatt ccggagatgg agatcagttt ctcgaacgcg
     7561 aacttctcct cgccgctcga aaagccgtag agcgagagag cgtcttcgcg gacatgcgtg
     7621 aaaatctcca gcgccaccga cttctgccct tcgagtttgt agtacgtctg cagggagatg
     7681 tgaatctcgt atccgacgcc gcccgcgagc acgagcgccc gcgtggcgtc gagctgtttg
     7741 agcgtcccct cgaggtaacc aatcatgatc cccagagtct accccgagga gtgcaggcgt
     7801 ctcgcttccc tgtagtgggt caatttgaaa acccggccac cacagaggca cagaggtcac
     7861 agagatctcc tctgtgttct ctgtgtctct gtggttgcct ttgcgccggc gtccacgata
     7921 cgcgtccaaa actggcccat taccgatgtt gcattcaccc gcaaactacc gccccccagc
     7981 cggccggttg gggggcggcg catgcgggag ctacttcttc gtcacgtcgt cgagcgcttt
     8041 gtcgaagaga tccgcgacgt tttccggctg gtcggttttg gccggggccg gacggggcgc
     8101 catgaaaccc ttcgcgccgg gcggcagttc gg
//
LOCUS       DATLVW010000442        17842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_268663, whole genome shotgun sequence.
ACCESSION   DATLVW010000442 DATLVW010000000
VERSION     DATLVW010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17842
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268663"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..516
                     /locus_tag="VNN25_13435"
     CDS             40..516
                     /locus_tag="VNN25_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXH92579.1"
                     /translation="MNAPILDEHDLADALARERSAGKTIAFANGCFDVLHVGHLRYLQ
                     DAARQADVLVVGINGDASVGELKGPGRPVMSEAERAELISAIRGVSYVTIFHDLSPGR
                     LIGNIKPDVHCKGTDYTPDSVPEAEIVRAYGGRVAIVGDAKEHSTTEILKRMKAEG"
     gene            513..1460
                     /gene="waaC"
                     /locus_tag="VNN25_13440"
     CDS             513..1460
                     /gene="waaC"
                     /locus_tag="VNN25_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HXH92580.1"
                     /translation="MRDESLSSVHPSKLLVVRLSAFGDVIHTIPAVVALCDALPQIEI
                     AWAVEPAYAELVELVAGVRAIRVSLKKWSLARIREAMRRVRGFDDAIDFQGLIKSALI
                     ARVSGARLRYGFARDVIREKPAAWFVNRPITIERSSHVVEWNLALARALAPAIAGVPE
                     VDFSPFADDPSGKLGAFANRIVFLPGAGRPEKQWPAERFAELARRLGSDVLLACGPGE
                     EPIGRAIGAELAPPTTFRELAFLLRGARLVVGGDTGPLHLAAALGVPVAGLYGPTDPA
                     RNGPYGQLGNVIESFSTTKSMQSIDVDDVLRLVLKINSR"
     gene            1547..3262
                     /gene="pilB"
                     /locus_tag="VNN25_13445"
     CDS             1547..3262
                     /gene="pilB"
                     /locus_tag="VNN25_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HXH92581.1"
                     /translation="MSVRLGELLTKASLISQDQLKEALKLQKDTGGKLGETLIKLGFV
                     SEEDITECLSQQFGVPSISLVHFEIDPSVIKLIPADVARKYNILPVNKTGATITIAMA
                     DPTNVFAMDDIKFMTGYNVEPVVASELGIKSAIDNYYGTTSSLELKKVMEDLQQSESA
                     DLEVLEEEEDMDMAALADSAEEAPVVKLVNLILTDAIKRGASDIHIEPYEKEFRVRFR
                     IDGTLYEIMNPPLKLKDAITSRMKILAKLDISEKRLPQDGRIKLKMKLNDKNKELDFR
                     VSVLPTLFGEKIVMRLLDKDNLRLDMTKLGFEPESLVRFEEAIFKPWGMVLVTGPTGS
                     GKTNTLYSALSKVNSPEVNIMTAEDPVEFNLPGINQVQMKESIGLNFAATLRSFLRQD
                     PNIILVGEIRDFETAEIAIKAALTGHLVLSTLHTNDAPSTINRLMNMGIEPFLVATSV
                     QLIAAQRLARRVCANCKEPVEMSPQALMNIGFRKDEVGSFTVYKGRGCDKCNSTGYKG
                     RVGLIEVMGIDDTIRELILSGGTAIDIKKRAAENGMISLRRSGLIKIKDGVTTIEEVV
                     RETIL"
     gene            3438..4565
                     /locus_tag="VNN25_13450"
     CDS             3438..4565
                     /locus_tag="VNN25_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXH92582.1"
                     /translation="MPVTLHQLLKTMVERGGSDLHVTTNSAPQIRIDGKLTPLDMPVL
                     TAPETKQLAYSVLTDAQKHRFEENLELDLSFGIKGLARFRANIFNQRGATAAVYRQIP
                     YEILGFRELGLPTVVEDICNRPRGLVLVTGPTGSGKSTTLAAMIDKINRERHEHIITI
                     EDPVEFLHSHKSCIVNQRELHADTHSFANSLKSALRQDPDVVLIGEMRDLETIESALR
                     IAETGHLTFGTLHTNSAAQTINRVVDVFPSHQQPQIRAQLSFVLEGIMCQSLLPKANG
                     KGRVMAMEILVPNAAIRNLIREDKVHQIYSMMQTGQAKYGMQTFNQSLATLYFKKHIT
                     LQVALARSSNPDELQEMISRGAGVLTPQNTPPSAGSRPRTA"
     gene            4590..5789
                     /locus_tag="VNN25_13455"
     CDS             4590..5789
                     /locus_tag="VNN25_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXH92583.1"
                     /translation="MPTFEWKGSRAGQMQSGVLTADSKDAAEQILRRQRIEAPSIKEK
                     GKEIALPKFGGGVAPQLVAIFTRQFSVMIDAGLPLVQCLEILAGQQENKTFKRALIQI
                     RQDVESGSNLADSMRKHPKIFNDLFTNMVAAGEAGGILDTILQRLAQYIEKSVKLNSQ
                     VKSAMIYPVAVISIACIVIAIILWKVIPVFAALFKGLGAELPMPTQIVIWLSNFIADY
                     WWLIALIIGGTIYSLRRYHETYKGKRVLDGIVLKMPILGMLMRKIAVARFCRTLSTLT
                     ASGVPILDGLQITARTSGNAIVEDAIMATRKSVEEGKTISEPLADTDVFPSMVVQMIA
                     VGEQTGALDTMLSKIADFYEDEVDTAVAGLMKLLEPVLIAFLGVAIGGIVIAMYMPMF
                     TLIGKVG"
     gene            5909..7549
                     /locus_tag="VNN25_13460"
     CDS             5909..7549
                     /locus_tag="VNN25_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH92584.1"
                     /translation="MHTDARMLIAVRVVAVTTLLLATLIIQYTVRELLPINYLYTTAG
                     VTYGLTLAYIGVGRLLKSRKLNLVIQISGDLVVETVLVYFTGGLDSPFSFLYLVSIIT
                     ASMLLYRRGGLLAASGAVILYGALADLMYYGVIPLPEQSWFVPTPWSSSRLYFNMATN
                     FAGFYATAFLTSYLAEKLRKTFEELDANRQNLAELRALNQNVVESIPSGLITLSQEGI
                     VTFINPAGCFILHQDAQSVIGHRIEQFGFYAEGEWQGVPPTLTAEPVVRSEKNDFQIG
                     DETRSLGFALTPLNSIEGMAAGYTLIFQDLTDMKKLEAELRLKDRMAAVGELSAGIAH
                     EIRNPLAAIAGSVQVLKKSAALNPQEQRLMSIILKESGRLNKSIADFLRFVKPQEKRP
                     AEFDIAASLSETLDLLGNSPELGAGHTIEHAITPPSYTIIGDGDQIRQVFWNIARNAV
                     QAMPDGGTLLVTTEVGGDQYNIVFSDNGRGMNDADQRRLFQPFRTNFPSGTGLGMAIS
                     YRIVQEHGGRIDVTSREGVGTAITVSLPVSASIRNAVA"
     gene            complement(7550..8443)
                     /locus_tag="VNN25_13465"
     CDS             complement(7550..8443)
                     /locus_tag="VNN25_13465"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXH92585.1"
                     /translation="MTIVCAAMNRDQLLEQWRAEGWLHLPRFFGETEISGINRVVDSL
                     WTAKPRDVTVDDVDRKVRCRMSELADSDRAHRVKISDLYLASPPVREMLLDRRLLDVV
                     EGLLDDAPVLCNSLNLEKSSGQEYHADSLYMTPLTEEGVVACWIALEDVRPGSGPLRL
                     YPASHLIAPFVFADGTRHAHDEEMPQWAGYMQSAIDSRGLGSVSVYAKAGDLVIWHSD
                     LLHGAEPITEPDSTRRSLVAHYYRHADCVRRGYLVEGSDNTFWWRRRPQPVDWFTRAQ
                     CAVERRVQRMRAAIRSRTKAH"
     gene            complement(8440..9237)
                     /locus_tag="VNN25_13470"
     CDS             complement(8440..9237)
                     /locus_tag="VNN25_13470"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH92586.1"
                     /translation="MNGNSSGTAGKAERAVPESDRIRWSTVRDSLTQPYRVTVSMVVL
                     VSLVPCYIFIADLIPKRALHVPALAMDALVPLQPIWTLVYAPLYLFVIMLPVFVVRQQ
                     EQIRRTVHAYLLVWITAFAFFLAYPTMAPRPLKVIGEGFAFWSLRFLYSLDPPYNCFP
                     SLHVAHSFVSALTCYRVHRGVGIAAGIAASLIGISTLFTRQHYIADVVAGTFLAFVAY
                     VVFLHGCRRDAVPELERRLAPVLALGIISFIALVLGGFWIAYRVTGE"
     gene            complement(9242..9706)
                     /locus_tag="VNN25_13475"
     CDS             complement(9242..9706)
                     /locus_tag="VNN25_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92587.1"
                     /translation="MAQFLLVTTLLSIIGLIGTSVVGFTASPGHIANHIFWSLATVIL
                     GLFSQSMTMFFFIGTGKEIKDAAKNDDAVVRRTKAFKAKVFPIATYAMAVLMVTFIMG
                     GGVASGKTPHWLHLTLVIASIAMYCRAYWVQLHAMIENATLMETYLRDDKLD"
     gene            9764..10828
                     /gene="purM"
                     /locus_tag="VNN25_13480"
     CDS             9764..10828
                     /gene="purM"
                     /locus_tag="VNN25_13480"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXH92588.1"
                     /translation="MTSKSSYRDAGVNIDAQDEALREVRRIARGTFTPDVVSDIGSFG
                     GLFRASFDGMEKPILVSSTDGVGTKLRVAQAMKIHDTVGYDLVCHCVNDILVQGARPL
                     FFLDYYATGRLRPPVMTDVVRGMARACSENGCALIGGETAEMPGFYGDDDYDIAGFIV
                     GVVDEKHLLTGKDVREGDILIGLPSIGLHTNGYSLARRILFDQLGHDHQTILPEVGKI
                     GEVLLAPHISYRKELLPLIDQNALHALAHITGGGLTDNVPRVLPDTLDAHIKLGSWPV
                     LPIYTFLAQHSDTSREEMLRVFNMGIGMVVIAAKEQVDAIVRHFGQIGQKFFFIGNVV
                     KGSGKVVYDKPPAGFASWIE"
     gene            10936..11535
                     /gene="purN"
                     /locus_tag="VNN25_13485"
     CDS             10936..11535
                     /gene="purN"
                     /locus_tag="VNN25_13485"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXH92589.1"
                     /translation="MRLAILLSGRGSNFLAIHQAITRGELHADIVCVISNRADAPGVE
                     RARSLGLSAFVVDHRKFASRAAHEAEVLRILGPANPDLIVLAGYMRLLSSEFVAAYPM
                     RIVNIHPSLLPSFPGVDAQAQAVAYGVKVSGCTVHFVDENLDAGPIIVQRIVPVRDDD
                     DASTLAARILDEEHVAYVEALKKLASGTYRVDGRRVVFD"
     gene            complement(11558..12742)
                     /locus_tag="VNN25_13490"
     CDS             complement(11558..12742)
                     /locus_tag="VNN25_13490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXH92590.1"
                     /translation="MKHCFAVLMIAAFATAVFGQEEEGTGAAKGAQVDMTGKGYSAAY
                     VIEPVSKRVLLEENAHVPLPTASMAKMMTCILAMERIQSGELKLDTPVTVSARASKIG
                     GSQIFAKEGQSFPMQTLLAATMIRSANDTAEMIAEKIGGSAEAFADLMNDKARQLGLK
                     ETTFYDPHGLPNSQEPKRIDVSSAHDLAIMGIELMKYPLMREYAKTAWMPFTNASYTS
                     GLTNPDHMINPKSRDYYGDAIGIKTGYSAPAGYCITAAAKRGDLEIIAVVMGARAASG
                     PQSSFGIAARLFNEAFAHWRQMTPLHKGMVVGQAAVAEGQAKTVAAVAAGDVQTLVPR
                     GQEGTINVKFVPSNLTAPVSAGQQVGMIIVNQGGQQIGRAPAVAQTGVEKQSWWKKFW
                     PF"
     gene            complement(12772..13077)
                     /locus_tag="VNN25_13495"
     CDS             complement(12772..13077)
                     /locus_tag="VNN25_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92591.1"
                     /translation="MTLVNSLCAEISIYQGVGEQTGVFVERSAIRGKKGLCKSMRDLA
                     IKSRVLGDGYYYIPTELWPDTKEPVTVHKHDWTVIGSIPRTSRAARKPLAESRPESA"
     gene            complement(13238..13894)
                     /locus_tag="VNN25_13500"
     CDS             complement(13238..13894)
                     /locus_tag="VNN25_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92592.1"
                     /translation="MQPVKPNQPTEAPAATDAQTLDAIASQLLDLVIKMEGLVPDFEP
                     HDKRAIARVAASAKFAHELIAPTITMVTSVPSVPQDLFNVAQAQEALQFRDLLRPIAQ
                     RLAAFAQGLEFTIDTKLSASGEDSLQSYHWGKRAVKGPSGAILQPYVDEMRRVVKRAI
                     NRSNKPKTEPPAPSPAPHGQTFMAIMPQRPAEDPAADADTDDGAELPESYYKAAEDEE
                     "
     gene            14463..14759
                     /locus_tag="VNN25_13505"
     CDS             14463..14759
                     /locus_tag="VNN25_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92593.1"
                     /translation="MGGVVLRFGGIAISKKEIAISRKEIDLRLGGVVLRFGGVAISKK
                     EIAISRKEIDLRLGGVVLRLGGVAISKKEIAISPAEMRISCREMGISPKEIAIS"
     gene            15030..16883
                     /locus_tag="VNN25_13510"
     CDS             15030..16883
                     /locus_tag="VNN25_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92594.1"
                     /translation="MAHHGSEKEKTKKKMDKARFRAILKDAGEIIWRSRARLAIGIPL
                     LLVNRLSSIVLPYMSKLLYDDVIGKGHVDLLPKLVLVSLAVAIFGGSTDYALAQILGI
                     AAQRSITDLRTRLQQHVQRLPIRYFDSTKTGVLVSRVMNDAEGIRNLVGTGLAQIFGG
                     VVTAGFATFFLFHLSARLASMVFLAFFAFAAILFWAFSTARPLFKQRGAVNAEVTGRL
                     TESFSGIRIVKAYTAEEHEEKVFEAGARKLLNLIVGTMRTISTAGAMTTFLVGAVSAV
                     VMYVGGHQVVLHKANPLVGMTPGDLIAFTLYLALVIGPVVQIVSIGTQLSEAFAGLER
                     MREVFGEKREDEDDAAKNQTEDIAGSIEFRDVWFEYTAGVPVLKNINVVAPAGKSIAL
                     VGPSGSGKSTMISLIAAFHHPTQGEILVDGKPMNDLRLADYRSHLGIVPQDSFLFAES
                     IYDNIALGNPQASREEVLYAAHIAHVDEFTEGFADKYETIVGERGVRLSGGQKQRVAI
                     ARAIVANPRILILDEATSSLDSESEALIQDGLNALMQGRTTFVIAHRLSTIRNADQIL
                     VLEGGEITERGTHSELMLLGGRYRKLYEKQYGVMINRFVNEGEELRDLAAK"
     gene            complement(17391..>17842)
                     /locus_tag="VNN25_13515"
     CDS             complement(17391..>17842)
                     /locus_tag="VNN25_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92595.1"
                     /translation="VIAGAADESAPGIVALLEKDKATQVFSVLPATRRDLNALQGDIG
                     TWPIPSLAFLRGTVLGAALWDSDARKRPVRIEDQFDALLYLGPPTSMTMSRLAPALCA
                     DPEYMQMRLGRLAPLAPPPGARFTPADQLKQYCAQAATAKKADVPNP"
BASE COUNT         3538 a         5561 c         5320 g         3423 t
ORIGIN      
        1 gttgcacaaa gcaatcgagc gtgatgaggc gctgcgcgag tgaacgcgcc gatcctcgac
       61 gagcatgatc tcgcggacgc actggcgaga gaacgttccg cgggcaagac catcgccttc
      121 gctaatggct gcttcgacgt actgcacgtc ggccatcttc gctacctgca ggatgccgcg
      181 cgtcaggcgg atgtgctcgt tgtcggcatc aacggagacg cgtccgtcgg cgagttgaag
      241 gggccgggcc ggcccgtgat gagtgaggcc gagcgcgctg agctgatctc ggcaatacga
      301 ggcgtctcct acgtcacgat ctttcacgat ctcagtccgg gacgattgat cggcaacatc
      361 aagcccgacg ttcactgcaa gggaaccgac tacacgccgg atagcgtgcc cgaagcggag
      421 attgtgcggg cgtatggcgg gagagtggcg atcgtgggag atgcgaagga gcactcgacg
      481 acagagatat tgaagaggat gaaggcggaa ggatgaggga tgagtcgctc agttccgttc
      541 atccttcgaa gcttctcgtc gtccgcctct ccgctttcgg cgatgtcatt cacaccatcc
      601 ccgccgtcgt ggcgctttgt gacgcgctgc cgcaaatcga gatcgcctgg gcggtcgagc
      661 cggcgtatgc ggaactagtc gagctcgtcg ccggtgtgag ggcgattcgc gtctcgctga
      721 agaagtggtc gctcgctcgg atccgcgagg ccatgaggcg cgtccgcggc tttgacgatg
      781 ccatcgactt tcagggcctc atcaagtccg cactaatagc gcgcgtcagc ggcgcgcgcc
      841 ttcggtacgg ttttgcacgg gacgtcatcc gcgagaagcc ggcagcgtgg ttcgtcaacc
      901 ggcccatcac gatcgaacgt tctagccacg ttgtcgagtg gaacctcgct ctcgcccgcg
      961 cgcttgcacc tgcgattgca ggcgtacccg aggtcgattt ctctccgttc gccgacgacc
     1021 cttccggcaa actcggcgca ttcgccaatc gcatcgtctt tcttccgggc gccggacggc
     1081 cggaaaagca atggcccgcc gagcggttcg ccgagcttgc ccgccgcctc ggcagtgacg
     1141 tcctcctcgc atgcggtccg ggagaagagc cgattggccg cgcgatcggc gccgagctcg
     1201 cgccgcccac gaccttccgc gaattggcgt tccttctccg cggcgcaaga ttagtcgttg
     1261 gcggcgacac cggcccgctg cacctcgccg ccgcgctcgg cgttcctgtc gccggcctct
     1321 acggtccgac cgatccggcg cgcaacggac cctacgggca actcggcaac gtgatcgagt
     1381 cattcagcac gacgaagtcg atgcaatcga tcgacgttga tgacgttttg cggctcgtac
     1441 tcaaaataaa ctcgcgttga cggtgattcg ggcagggagg taaactcgcc ggtcatctgt
     1501 cgaaatcact catgccccgc aacttagcag caccccactt aaacctatga gcgtacgtct
     1561 cggagagctt ctgaccaaag cgagtctgat cagccaggat cagctcaagg aagcgctcaa
     1621 gcttcagaaa gacactggcg gaaagctcgg tgagacgctc atcaagctcg gcttcgtctc
     1681 tgaagaggac atcaccgagt gcctctcgca acaattcggc gttccgtcga tcagcctcgt
     1741 ccatttcgag atcgacccct cggtcatcaa gctgattccg gccgacgtcg cgcgcaagta
     1801 caacatcctt cccgtcaaca agacgggggc gacgatcacg atcgccatgg cggatccgac
     1861 gaacgtcttc gccatggacg acatcaagtt catgaccggc tacaacgtcg agccggtcgt
     1921 ggcctcggag ctcggcatca agtccgccat cgacaactac tacggcacga cctcgtcgct
     1981 ggagttgaag aaggtgatgg aagacctcca gcagtcggag agcgccgatc tggaggttct
     2041 cgaagaagaa gaagacatgg acatggcggc gctcgccgac tcggcggagg aagcgccggt
     2101 cgtcaagctc gtcaacctca tcctgacgga cgccatcaaa cggggcgcgt cggacatcca
     2161 catcgagccc tacgagaaag agtttcgcgt gcgcttccgg atcgacggga cgctgtacga
     2221 gatcatgaat ccgccgctga aactgaaaga cgcgatcacg tcgcgcatga agattctggc
     2281 gaagctcgac atctccgaga agcgactgcc gcaggacggc cgcatcaagc tgaagatgaa
     2341 gctcaacgac aagaacaaag agctcgactt ccgcgtctcg gtgcttccga cgctcttcgg
     2401 cgaaaagatc gtcatgcgcc ttctggacaa ggacaacctt cgtctggaca tgaccaagct
     2461 cggcttcgag ccggagtcgc tggtacgctt cgaagaagca atcttcaaac cgtggggaat
     2521 ggtcctcgtg accggcccga ccggttccgg aaaaaccaac acgctctatt cggctctttc
     2581 gaaagtgaac tcgcccgaag tcaacatcat gaccgcggag gacccggtgg agttcaatct
     2641 gccgggcatc aaccaggtgc agatgaagga gtcgatcggg ctgaatttcg cagccacgct
     2701 ccgctcgttt ctccgccagg atccgaacat catcctcgtc ggcgagatcc gcgacttcga
     2761 gaccgccgag atcgcgatca aggcggcgct cacgggccac ctcgtgctct cgacgctgca
     2821 cacgaatgac gcgccgtcga cgatcaaccg ccttatgaac atgggaatcg aacccttcct
     2881 cgtcgccact tccgtgcagc tcatcgccgc gcagcgcctt gctcggcgcg tctgcgcgaa
     2941 ctgtaaagag cccgtcgaaa tgtcgccgca ggcgctgatg aacatcggct tcaggaaaga
     3001 tgaggtcggc tctttcacgg tttacaaggg acgcggttgc gacaagtgca acagcacggg
     3061 ctacaaagga cgcgtcggcc tcatcgaggt gatgggcatc gacgacacga ttcgcgagct
     3121 catcctctcc ggcggcaccg ccatcgacat caagaaacgc gccgctgaaa acggcatgat
     3181 cagcctgcgc cgctccggac tgattaaaat caaagacggc gttacgacga tcgaagaggt
     3241 cgttcgggag acgattttgt gagggagtcg cgtttcggga gtcgggcgtc ggagaatcgt
     3301 cattccgaag cccagcaccc ggagctcgac actcggactt ttgcgattgg gggttcggtg
     3361 tggtgcatcg tgagtcggaa aggcattcgt agtctccgac acccgacacc cgacacccga
     3421 cacccggaaa gacaccaatg ccagtcaccc ttcatcaact tctgaagacg atggtcgagc
     3481 gcggcggctc cgatctgcac gtcacgacca actccgcccc gcagatccgc atcgacggaa
     3541 agctcacgcc gctcgatatg cctgtgctca ccgcgccgga gaccaagcag ctcgcttact
     3601 ccgtcctcac ggatgcgcaa aagcaccgct tcgaggaaaa cctggagctc gacctctcct
     3661 tcggcatcaa agggctggcc cgcttccgcg ccaacatctt caaccagcgc ggcgccacgg
     3721 ccgccgtcta ccgccagatt ccctacgaga tcctcgggtt tcgcgagctc gggctgccca
     3781 cagtcgtcga ggatatctgc aaccgtccac gtggtctcgt gctcgtcacc ggcccgaccg
     3841 gctcgggcaa gtcgaccacc ctcgcggcga tgatcgacaa gatcaaccgc gagcgccacg
     3901 agcacatcat caccatcgag gatcccgtcg agtttctgca ctcccataag agctgcatcg
     3961 tcaatcagcg cgagcttcac gccgacacgc attcattcgc gaactcactc aagtcggcgc
     4021 tgcgccagga ccccgacgtc gtgctcatcg gcgaaatgcg tgacctcgag acgatcgagt
     4081 ccgcgctgcg catcgccgag accggccacc ttaccttcgg aacgcttcac acgaattcgg
     4141 ctgcgcagac catcaaccgc gtcgtcgacg ttttcccgtc ccaccagcag ccgcagatcc
     4201 gcgcccagct ctccttcgtt ctggaaggga tcatgtgcca aagccttctt ccgaaagcga
     4261 acggaaaggg gcgcgtcatg gccatggaga ttctcgtgcc gaacgccgcc atccgaaatc
     4321 tgatccgtga agacaaggtg caccagatct actcgatgat gcagaccggt caggccaagt
     4381 acggaatgca gactttcaac cagtcgttgg cgaccttgta cttcaagaag cacattacgc
     4441 tgcaggtggc gctcgctcgt tcctcgaatc ctgacgagct ccaggaaatg atttcccgcg
     4501 gcgccggcgt gctcacgccg caaaacactc cgccgtccgc gggcagccgt ccacgcacgg
     4561 cgtgaaacag tcgttaacgg gagggaatga tgcctacgtt cgaatggaag ggatcgcgag
     4621 ccggccagat gcagagtggc gtcctgaccg ccgattccaa agacgccgcg gaacagatcc
     4681 ttcgccgcca acgcattgaa gcaccgtcga tcaaagagaa ggggaaggag atcgcgctgc
     4741 cgaaattcgg cggaggcgtg gccccccagc tagtcgccat cttcacgcgc cagttctccg
     4801 tcatgatcga tgccggcctg ccgctcgtgc aatgcctcga gattctggcc ggccagcagg
     4861 aaaacaagac cttcaagcgc gctctcattc agattcgtca ggatgtcgag tcgggctcca
     4921 acctcgccga ttccatgcgc aaacatccga agatcttcaa cgatctcttc acgaacatgg
     4981 tcgccgccgg tgaggcgggc ggtattctcg acacgatcct ccagcggctg gcgcagtaca
     5041 tcgaaaagtc ggtcaagctc aacagccagg tgaagtcggc gatgatttac ccggtcgcgg
     5101 tcatctccat cgcctgcatc gtcatcgcca tcattctctg gaaggtcatc ccggtctttg
     5161 ccgctctttt caagggcctg ggagcagagc ttccgatgcc gacgcagatc gtcatctggc
     5221 tctcgaattt catcgccgac tactggtggc tcattgcgct catcatcggc ggcaccatct
     5281 actccctgcg ccgttatcac gagacgtaca aagggaagcg cgttctcgat ggaatcgtcc
     5341 tgaagatgcc cattctcggg atgctgatgc gcaagatcgc tgtggcccgc ttctgccgta
     5401 cgctctcgac cctcacggcc tcgggcgttc ccatcctcga cggactgcag atcaccgccc
     5461 gcacctccgg aaacgccatc gtcgaagatg ccatcatggc cacccggaaa tcggtcgaag
     5521 aaggcaagac catcagcgag ccgctggccg acaccgacgt cttcccgtcc atggtcgtcc
     5581 agatgatcgc ggtcggtgaa cagaccggcg cactcgacac gatgctgtcg aagatcgctg
     5641 acttttacga agacgaagtc gataccgccg tggccggcct catgaaactc ctcgagccgg
     5701 tgctcatcgc ctttctcggc gtcgccatcg gcggcatcgt catcgccatg tacatgccga
     5761 tgttcactct gatcggaaag gtcggttagc ggtcgtcgct gaagaacgat gcaccacaga
     5821 gacgcagagc tcacagagtt gtagtggctg actctgtgac ctctgtgtct ctgtggtgaa
     5881 aggctttcac gccagcatca caacacccat gcacaccgac gcccgcatgc tgattgccgt
     5941 acgcgtcgtc gccgtgacga cgctgctgct cgctacgctc atcattcagt acacagtccg
     6001 cgagctgctg ccgatcaact atctctacac caccgcgggc gtcacgtacg gactcaccct
     6061 cgcgtacatc ggcgtgggca ggctcctcaa atcgcgcaag ctgaatctcg tcatccagat
     6121 ctccggcgat ctcgtcgtcg agacggtgct cgtctatttc accggcggtc tcgactcgcc
     6181 gttctcgttt ctctacctcg tctcgatcat caccgcgtcg atgctgctct accggcgcgg
     6241 aggccttctc gccgccagcg gtgctgtcat tctgtacggc gcgctcgccg acttgatgta
     6301 ctacggcgtg atccctcttc ccgaacaatc gtggttcgtg ccgacgccgt ggagctcatc
     6361 gcgcctctac ttcaacatgg ccacgaactt cgccggcttc tacgctacgg cgttcctgac
     6421 ctcatatctc gcggagaaac tgcggaagac gttcgaagag cttgatgcca accgccagaa
     6481 ccttgcggag ctgcgggctc tgaaccagaa cgtcgtcgag tcgatccctt caggcctgat
     6541 cacactgtcg caggaaggca tcgtcacgtt catcaacccc gcggggtgtt tcattcttca
     6601 tcaggacgcg cagtcggtga tcggtcacag gatcgaacaa tttggtttct acgccgaggg
     6661 ggagtggcag ggggttcctc caacactgac tgccgagccg gtggtccgaa gcgagaagaa
     6721 tgatttccag atcggcgacg agacgcgcag tttgggtttc gctctcacgc cactcaactc
     6781 catcgaggga atggccgcgg gatacacgct catcttccag gacctcaccg atatgaagaa
     6841 gctcgaagcc gagcttcggc tgaaagaccg catggccgcc gtcggcgagc tctcggcggg
     6901 tatcgcccat gagattcgca accccctcgc tgccatcgcc ggctcagtgc aggtgctcaa
     6961 gaaatcggcc gcgctcaatc ctcaggaaca gcggctcatg tcgatcattc tgaaggaatc
     7021 cgggcggctg aacaaatcga tcgccgactt tctgcgcttc gtgaaaccgc aggagaaacg
     7081 ccccgcggag ttcgacatcg ccgccagcct ttccgagact ctcgacctcc ttggaaactc
     7141 tcccgagctc ggcgcaggtc acacgatcga gcacgcaatc acaccgccgt catacaccat
     7201 catcggcgac ggcgatcaga tccggcaggt cttctggaac atcgctcgca acgcagtgca
     7261 ggccatgccc gacggtggaa cactgctcgt cacgaccgag gttgggggcg accagtacaa
     7321 catcgtgttc agtgataatg gccgaggcat gaacgacgcc gatcagcggc gccttttcca
     7381 gcctttccga acgaatttcc cttccggcac cggcctcggc atggccatct cctaccgaat
     7441 cgtgcaggag catggcgggc gcatcgatgt gacctcgcgc gaaggagtcg gcactgcgat
     7501 tacggtgtcg ctgcctgtat cggcgtctat tcgtaatgcg gtggcctgat tagtgcgcct
     7561 tcgttcgcga gcgaatggcg gcgcgcattc gctggacgcg ccgctctacc gcgcactgcg
     7621 cacgcgtgaa ccaatcaaca ggttgcgggc gccggcgcca ccagaatgta ttgtctgatc
     7681 cctcgaccaa atacccgcgc cggacacaat cggcatgtcg gtaatagtgc gccaccagac
     7741 tccgccgggt actgtctggc tctgtaatcg gttcagcacc gtgcagcagg tccgaatgcc
     7801 agatcacgag atcgccggcc ttcgcatata ccgagaccga accgagacca cgcgaatcga
     7861 tggccgactg catgtagccc gcccactgtg gcatttcctc gtcatgcgcg tggcgcgtgc
     7921 cgtctgcaaa cacgaacggc gcaatcaggt ggcttgccgg atagaggcgc agcggcccgg
     7981 agccgggtcg aacatcttcc agcgcaatcc agcacgcaac cacgccttct tccgtcaaag
     8041 gcgtcatgta gagcgagtca gcgtggtact cctgcccgct gctcttctcc agattcaacg
     8101 aattgcaaag cacaggagcg tcatcaagca gcccttcaac gacatcgaga aggcgacgat
     8161 ccagcagcat ctcgcgaaca ggcggtgatg cgagatagag atcgctgatc ttcactcgat
     8221 gcgcgcggtc gctgtcagcc agttccgaca tccggcagcg cactttccga tcgacgtcgt
     8281 cgacagtgac atcgcgcggc ttcgctgtcc agagagagtc gacgacgcga ttgatcccac
     8341 tgatctcagt ctcaccgaag aaccgcggca aatgcagcca accctcggcg cgccactgct
     8401 cgagcaactg gtctcgattc atcgcagcgc aaactatagt cattcccctg ttacgcggta
     8461 ggcaatccag aagccgccta gcacgagggc gataaagctg atgatgccaa gcgcgaggac
     8521 cggtgcgagg cgccgctcga gctcgggaac cgcgtcgcgc cgacagccgt gcagaaacac
     8581 cacatacgcc acaaacgcga ggaaagtgcc cgcgaccaca tcggcaatgt agtgctgcct
     8641 ggtaaacaac gtcgagattc cgatgagcga ggcggcgatt ccggctgcga tcccaacgcc
     8701 gcggtgtacg cgataacagg tcagggccga cacgaaggag tgcgcgacgt gcaatgacgg
     8761 gaagcagttg tagggcgggt cgagcgaata gaggaagcgc aggctccaga acgcgaatcc
     8821 ctccccaatg actttcaacg ggcgcggtgc catcgtcgga tatgccagaa agaaggcgaa
     8881 cgcggtaatc cacaccagca gataggcgtg aacggtgcga cggatctgct cctgttgccg
     8941 cacgacaaac accggcagca tgatcacgaa cagataaagc ggcgcataga cgagcgtcca
     9001 gataggttga agcggcacga gcgcgtccat tgccagcgcg ggcacgtgca gcgcgcgttt
     9061 cggaatcagg tcggcgatga agatgtagca cggcacgagc gacacgagca cgaccatgga
     9121 aacggtgacg cgatagggtt gcgtcagcga atcccgaacc gtgctccatc ggatgcggtc
     9181 cgattcggga actgcccgct cagcttttcc agcagtgccg ctgctgtttc cgttcatcag
     9241 atcaatcgag tttatcgtct cgcagatacg tctccatcaa cgtggcgttt tcgatcatcg
     9301 cgtgcagttg tacccaatag gcacggcagt acatcgcgat ggaggcgatg acgagcgtga
     9361 ggtgcaacca atgcggcgtc ttcccggatg ccacaccgcc gcccatgatg aacgtcacca
     9421 tcagcacggc catcgcgtag gtcgcgatcg ggaaaacctt cgctttgaat gccttcgtac
     9481 ggcgcacgac agcatcgtca ttcttcgccg catccttgat ctccttgccg gtgccgatga
     9541 agaagaacat cgtcatcgac tgcgagaaga gcccgaggat cacggtggca agcgaccaga
     9601 aaatgtgatt ggcgatgtga cccggcgacg ccgtgaaacc gacgacgctg gtgccgatca
     9661 acccgatgat ggaaaggaga gtggtgacga gaaggaactg tgccaagcga ggggattata
     9721 ggttggggct ggcctcgcga gaccgctgat acacttcccc gccgtgacct cgaaatccag
     9781 ttaccgcgat gccggcgtca acatcgacgc gcaggacgag gcgttgcgcg aggttcgcag
     9841 gatcgcccgc gggacgttca cgccggatgt cgtctccgat atcggctcgt tcggtggact
     9901 cttccgcgcg tccttcgacg gaatggaaaa gccgattctc gtttcctcga ccgatggcgt
     9961 cggcacgaag ctacgcgtgg cgcaggcgat gaagattcac gacaccgtcg gttacgacct
    10021 cgtctgccac tgcgtcaacg acattctcgt tcagggtgcg cgccctctct tctttctcga
    10081 ctactacgcc accggcaggc ttcggccgcc cgtgatgacc gatgtcgttc ggggaatggc
    10141 ccgtgcgtgc agcgagaatg gttgcgcgct gatcggcggc gagaccgcgg agatgcctgg
    10201 tttttatggc gatgacgatt acgacatcgc cggcttcatc gtcggcgttg tcgacgagaa
    10261 acatctgctg acgggcaagg atgtccgcga aggcgacatc ctcatcggcc tgccgtctat
    10321 cggacttcac acgaacggct attcgctggc gcggcgaatc ctcttcgatc agctcggcca
    10381 cgaccaccag acgatcctgc cggaggtcgg caagatcggc gaggtgctgc tggcaccgca
    10441 tatctcctac cgcaaagagc ttctgccgct catcgaccag aacgcgcttc acgccctcgc
    10501 ccacatcacc ggcgggggct tgaccgacaa cgtcccgcgc gttctgccgg acacgctcga
    10561 cgcgcacatc aagcttggct catggcccgt gcttccgatc tacacgttcc ttgcgcaaca
    10621 cagcgacacc agccgcgagg agatgctccg cgtcttcaac atgggcatcg gcatggtggt
    10681 catcgcagcg aaggagcagg tcgacgcgat cgtccgccac ttcggccaga tcggccagaa
    10741 gttcttcttc atcggcaacg tggtgaaggg aagcggcaaa gtcgtctacg acaagccgcc
    10801 ggcaggattc gcatcctgga tcgaatgaga ctcacttctt agaagcgttt cacgcggaga
    10861 cgcggaggac gcggggttga gtctccacga actccgcgtc tccgcgtgac aaatctctcg
    10921 taactaaaac ggcaaatgcg cttagcaatc ctcctctccg gccgcggctc gaacttctta
    10981 gcgattcacc aagcgatcac gcgaggcgag ctccatgccg acatcgtttg cgtgatctcg
    11041 aaccgcgccg atgcgccggg cgtcgagcgc gcgcgatcac tcggactgtc cgcattcgtc
    11101 gtcgatcacc gcaagttcgc cagccgcgca gcgcacgaag cggaagttct gcgcatcctc
    11161 ggcccggcca atcccgactt gatcgtcctg gccgggtaca tgcggctgct ctcgtccgag
    11221 ttcgttgccg cctacccgat gcgtatcgtg aacatccatc cctcgctgct tccctcgttt
    11281 ccgggagtgg atgcgcaggc gcaggctgtc gcttacggcg tgaaagtctc cggctgcacc
    11341 gtccacttcg tcgatgagaa cctcgacgcc gggccgatca tcgtccagcg catagtcccc
    11401 gtgcgcgacg acgatgatgc ctccacactc gcggcccgca tcctcgatga ggagcacgtg
    11461 gcgtacgtgg aagcgctgaa aaagctggcg tcaggaacgt atcgcgtcga cggccgaagg
    11521 gtcgtcttcg attgatcgtg aggtttgcgc taccgcatca gaacggccaa aacttcttcc
    11581 accacgactg tttctcgaca ccggtctgag cgacggcggg agcgcggccg atctgctggc
    11641 cgccttgatt cacgatgatc atgcccacct gctgtccggc gctgaccgga gcggtgagat
    11701 tgcttgggac gaacttcacg ttgatcgtcc cttcctgtcc gcgcggcacc agcgtctgca
    11761 cgtcgcccgc ggcgactgcg gccaccgtct tcgcttgtcc ctcagcgacc gcggcttgtc
    11821 cgaccaccat gcccttgtgc aggggcgtca tctgccgcca gtgcgcgaag gcctcgttga
    11881 acaggcgcgc ggcgatgccg aatgagctct gcgggcccga cgcagcgcga gcgcccatca
    11941 cgacggcaat gatctccagg tcgccgcgtt tcgcagcggc ggtgatgcag tagccggcag
    12001 gtgcggaata gcccgtcttg ataccgatgg cgtcgccgta gtagtcacgg ctcttcggat
    12061 tgatcatgtg atcgggattc gtgagtcccg acgtgtagct ggcgttcgtg aagggcatcc
    12121 aggccgtctt cgcgtactca cgcatcagcg gatacttcat cagctcgatc cccatgatgg
    12181 cgagatcgtg cgcgctggag acatcgatgc gcttcggttc ctgactgttc gggagaccgt
    12241 gcgggtcgta gaacgtcgtc tctttgagtc cgagctgacg agccttgtcg ttcatcagat
    12301 ccgcaaacgc ctcggcggac cctccgatct tctcggcgat catttccgcg gtgtcgttcg
    12361 cggagcggat catcgtcgcc gccagcagcg tttgcatcgg gaacgactgg ccttctttcg
    12421 cgaagatctg cgagccgcct atcttgctcg cgcgtgccga caccgtcacg ggagtgtcga
    12481 gtttgagctc tcccgactgg atccgttcca tggccaggat gcaggtcatc atcttcgcca
    12541 tcgaagcggt gggcagggga acatgcgcgt tctcttcgag caatacgcgt ttactgaccg
    12601 gctcgatgac ataggcggcg ctgtagccct tccctgtcat gtcgacttgt gcgcccttcg
    12661 cagcgcccgt tccttcctct tcctggccaa aaacggcagt agcgaaggca gcgatcatca
    12721 gtacggcgaa acaatgtttc atcgtgagtg ctccggaatt gatgttgttc ttcaggcgga
    12781 ctcgggccgc gattcggcga ggggctttct ggcagcgcgg gaggtgcgcg gaatcgatcc
    12841 gatcacggtc cagtcatgtt tgtgcacggt caccggctct ttggtgtccg gccagagctc
    12901 ggtggggatg tagtagtagc cgtcgccgag gacgcgcgac ttgatcgcca ggtcccgcat
    12961 gctcttgcac aaccccttct tgccccgaat cgcacttcgt tcgacgaaca ctccagtctg
    13021 ctcgccaact ccttgataga tactgatttc tgcgcaaagt gagttcacta acgtcattga
    13081 ggccactcca ttcagcagac tcaatagcaa gttctgaacc atggcgcgtc ggtcgcagtg
    13141 tgcctcgcat ccgggaacgt accggcactc ctgggaatcc tcaaaatgca ccgcccccga
    13201 agagtgctcg ggggcggtgc cagacaacag gtagtcgcta ctcctcgtcc tcagcggcct
    13261 tgtagtagct ctccggcagc tcggcgccgt catcggtatc agcgtcggca gcggggtcct
    13321 cggctggccg ttgcggcatg atggccatga aggtctgacc gtgcggcgcc ggcgatgggg
    13381 ccgggggctc ggtcttcggc ttgtttgacc ggttgatggc ccgcttcacc acgcggcgca
    13441 tctcatcgac atagggttgc aggatggcgc cgctcggacc tttgacggcg cgtttgcccc
    13501 aatggtacga ctgcagggag tcctctccgg aggcggagag tttcgtgtcg atggtgaatt
    13561 ccagcccctg tgcgaacgcc gccagccgct gcgcgatcgg tcggagcagg tcgcggaact
    13621 gcagcgcctc ctgcgcctgg gcgacgttga acagatcctg cggcaccgag gggaccgacg
    13681 tcaccatggt gatcgtcggc gcgatcagct cgtgagcgaa cttggcgctc gccgccacgc
    13741 gtgcgatcgc acgtttgtcg tgcggctcga aatcaggaac gagtccctcc atcttaatga
    13801 caaggtccag aagctggctg gcgatcgcat ccagcgtctg ggcgtcggtg gccgccggcg
    13861 cttcggttgg ttgattaggt ttaacaggtt gcatgagaca gtcctccctc tttcgcgtcc
    13921 ggtcggccgg aggcgaggtt gaataaatgg gcgccggtgg tcgccggcgc gtcggttggt
    13981 tgagtggttt tggttggttg catgagacaa acatcccctt ttcgcgtcac ggtcggccgg
    14041 acgcgaggtc gaacaaatgg acgccggtag ccgccggcgc gtcggttggt taagcggttt
    14101 tggttcattt ccaaagacag acctcctctt ttcgcgtcgc ggtcggccgg acgcgaggta
    14161 aatgggtgat gcgagctcgc cggatcgccc cgtcaaccac gcgttgacag agcgggctgt
    14221 aggtgtgacg gatcaggccg gtcgaaggtt acgggttcga gagagaaagt tgttcgcggg
    14281 ccgtccgcag caccggtctg agggggctct ggcgtcgaga aatcacgtcg atctgcggtg
    14341 ctgggggtgg ttctaagctc tggggggtcg atttaagatt cgggggggtc gttttaagat
    14401 tcgggggggt agcgatctca aaaaaggaga tcgcgatctc gagaaaagag atcgatctgc
    14461 gattgggagg ggtcgtttta agatttgggg ggatagcgat ctcaaaaaag gagatcgcta
    14521 tctcaagaaa agagatcgat ctgcgattgg gaggggtcgt tttgagattc gggggggtcg
    14581 cgatctcaaa aaaggagatc gctatctcaa gaaaagagat cgatctgcga ttgggagggg
    14641 tcgttttaag attgggaggg gtcgcgatct caaaaaagga gatcgctatc tcgccggcgg
    14701 agatgagaat ctcgtgcagg gagatgggga tctcgccgaa ggagatcgct atctcgtagg
    14761 ttgaaatcgc gatctcgtgg aaagagatcc ggatctcgta gatcgagatc gcgatctcgt
    14821 ggaaagagat cgggatctcc ccgaagcaga tcgtgacctc cccgagggag atcgcaatct
    14881 cgtgaaagga gattgcgacc tcggagagcc tgatcgcgac ctcctggctg ctggagacgc
    14941 actaaaggca tcggcgtctc ccatcccttc caagcactcc aaccacaccc cacgcccaac
    15001 cccccacccc caccgtctac aattccccga tggcacacca cggatcagag aaagagaaga
    15061 ccaagaaaaa gatggacaag gcccgcttcc gggccatcct caaagatgcc ggcgagatca
    15121 tctggcgctc gcgggcacgt ctggccatcg gcatccccct gctgctcgtc aatcgccttt
    15181 ccagcatcgt cctcccgtac atgtcgaagc tgctctacga cgacgtgatc gggaaagggc
    15241 atgtagacct gctgccgaag ctcgtgctgg tgagtctcgc cgtggcgatc ttcggcggct
    15301 caactgatta cgccctcgcg cagatcctcg gcattgccgc gcagcgctcg atcaccgatc
    15361 tgcgcacgcg cctgcagcag cacgtgcagc ggcttccgat ccgctacttc gactcgacca
    15421 agacaggggt tttggtctcg cgcgtcatga acgatgccga ggggattcgc aacctcgtcg
    15481 gtacgggcct cgcgcagatc ttcggcggcg tcgtcacggc cggtttcgct acgttttttc
    15541 tctttcatct cagcgcgcga ctggcgtcga tggtctttct ggcgttcttc gccttcgcgg
    15601 ccatcctgtt ctgggcgttt tcaacggcgc gtcctctctt caaacagcgc ggggcggtca
    15661 acgccgaggt gaccggacgt ctgacggaga gtttctccgg cattcgcatc gtcaaggcct
    15721 acaccgccga ggagcatgag gagaaggtct tcgaggcagg agccaggaaa ctgctcaacc
    15781 tcatcgtcgg cacgatgcgc accatctcca ccgccggcgc catgactacg ttcctcgtcg
    15841 gcgcggtcag cgcggtcgtc atgtacgtcg gcggacacca ggtggtcctg cacaaggcca
    15901 acccgctggt gggaatgacg cccggcgacc tgatcgcatt cactctctac ctcgcgctag
    15961 tcatcggtcc ggtcgtgcag atcgtctcga tcgggacgca gctctccgag gcattcgccg
    16021 gcctggagcg gatgcgcgaa gtcttcggcg agaagcgcga agatgaagac gatgccgcca
    16081 agaatcagac ggaggacatc gccggcagca tcgagttccg cgacgtctgg ttcgagtaca
    16141 cggccggcgt gcctgtgctg aagaacatca acgtcgtcgc gcctgccggc aaatcgatcg
    16201 ctctcgtcgg tccttcagga tcggggaaga gcacgatgat ctcgctcatc gcggcgttcc
    16261 accaccccac gcagggcgaa attctcgtcg acggcaagcc gatgaacgat ctccgtctcg
    16321 ccgactatcg ctcgcacctc ggcatcgtgc cgcaggactc cttccttttc gccgagtcga
    16381 tctacgacaa catcgccctc ggcaacccac aggcctcgcg cgaggaggtg ttgtacgccg
    16441 cgcacatcgc gcacgtcgac gagttcaccg agggcttcgc ggacaagtac gagacgatcg
    16501 tcggcgagcg cggcgttcgc ctctccggcg gacagaagca gcgcgtcgcc atcgcacgcg
    16561 ccatcgtcgc caacccgcga atcctcatcc tcgatgaggc aacgtcgtct cttgatagcg
    16621 agtccgaggc gctgattcag gatggattga acgcgctgat gcagggccga accaccttcg
    16681 tcatcgccca cagactgtcg acgatccgca acgccgacca gatcctcgtc ctcgaaggtg
    16741 gcgagatcac ggagcgcggc acccacagcg agctgatgct cctcggcggc cgctaccgca
    16801 agctgtacga gaagcagtac ggcgtgatga tcaaccgctt cgtgaacgag ggggaggagt
    16861 tgagggattt ggcggcgaag tagcggcagg tgcgccgaga ttcatgaaac cgacattgct
    16921 gagctcaatc gtgatgtgtc catggccgtc gtagttgccc tgatcacgag gattcatcgc
    16981 tcacttcgct ccatcgacgc aactagagtc actggtggct gcggccacac tcacggacga
    17041 aaggtgatgg tgggagattc cgccgcgacg atgttcacca catgatccga aaaaaacacg
    17101 cggtagtggc gaagcgacgc tggggctgga tcgttcttgg taaaacggcc gcggcaccgg
    17161 tcgagtgctt cagagttttc catgaggcag aaccgcgtgc ccactgactt ctccgttgca
    17221 ccctcgtcga agtgcgattc gtccaggcgc tagttgggcg gcgcgtccga tcgacacgaa
    17281 cttcagctac caccagaggg cgcacgtgac ttccgcaaat gcaaagaacc ggcgcgggct
    17341 gaaggcgtcg gtactccccg cgatctgggc gatgacgttc ggatgcgtcg tcatggattc
    17401 ggcacatcgg ctttctttgc cgttgcggcc tgcgcgcagt actgcttcag ctgatctgcc
    17461 ggcgtaaagc gggcgcctgg cggcggcgca agcggcgcga gccggccgag ccgcatctgc
    17521 atgtactcgg gatctgcgca gagcgcgggc gcaagtctcg acatcgtcat ggatgttggc
    17581 ggtcccaggt acaacagcgc atcgaactgg tcctcgatac gaacgggtcg ctttcgtgcg
    17641 tcggaatccc agagcgcggc gccgaggacc gttccgcgaa gaaacgccag gctcggaatc
    17701 ggccatgttc cgatgtctcc ctggagagcg ttcaggtctc ggcgagtcgc gggaagcacg
    17761 ctgaagacct gcgttgcttt gtctttctcc aggagggcaa cgattccggg tgccgattcg
    17821 tctgctgctc cagcgatcac gt
//
LOCUS       DATLVW010000443         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_389727, whole genome shotgun sequence.
ACCESSION   DATLVW010000443 DATLVW010000000
VERSION     DATLVW010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389727"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VNN25_13520"
     CDS             complement(<1..1711)
                     /locus_tag="VNN25_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXH92596.1"
                     /translation="MNDDVVRRHLSGRDDNGQPFVAGVYPMLLDEACFFLAADFDKTG
                     WREDASAFAETCRRMNLPAVIERSRSGQGGHVWLFFEEAIPAVLARRLGAHILTETME
                     RRPEVGLDSYDRFFPNQDTLPRGGFGNLIALPLQKRARELGNSLFVDETLTPYPDQWA
                     FLSTVHRITRSRVEDIVRDAERIGQIVGVRFPRADEDEEEPWTAPPSGYRKLPSIAGL
                     PRTLEVVLGNQVYIAKEGLVPALRNRLLRLAAFQNPEFYKAQSMRLATYDKPRIIACA
                     EDHPQHIGLPRGCLGDLLQLLADLKITPVVRDERLPGLPLRVAFRGELRPEQRIAADA
                     MLAHETGVLSATTAFGKTVIAAWLIAQRGVNTLVLVHRRQLLDQWVERLSAFLDLPAG
                     GIGQIGGGRDRANGSLDVALIQSLGRKGVVDDRVGEYGHLIVDECHHLPAYSFEQVAR
                     SAKARFVVGLSATVARKDGHHPILFMQCGPIRHRVNAKMQATARPFEHTVIVRPTAFQ
                     SADTAGADQRVAFQSLYRELIDDELRNQRICDDVVEAVHDGRSPLVLTERNDHLDHLA
                     AQLA"
     gene            complement(2582..2657)
                     /locus_tag="VNN25_13525"
     tRNA            complement(2582..2657)
                     /locus_tag="VNN25_13525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2622..2624),aa:Gly,seq:ccc)
     gene            complement(2728..3063)
                     /locus_tag="VNN25_13530"
     CDS             complement(2728..3063)
                     /locus_tag="VNN25_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HXH92597.1"
                     /translation="MRTTRAHPKAGTRQRRWSQRVTETSNALDLEKGVFSKRDPGAIA
                     RSLKRSADRSGRRKSNPYRSAMSMLTFYINRAGKHLTRNDRHRLEQAKDELRRVYRRP
                     ARGSGSSRS"
     gene            3051..3341
                     /locus_tag="VNN25_13535"
     CDS             3051..3341
                     /locus_tag="VNN25_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92598.1"
                     /translation="MSFAIDHSYCKFRVGRERFTLAASPQATFPLDRVRPHVRSARGA
                     REDAVLFPAIHMIVSPHEYDALGEEFEKKEKRSHSGVVRLLVRPHDGHHVHL"
     gene            complement(3381..3761)
                     /locus_tag="VNN25_13540"
     CDS             complement(3381..3761)
                     /locus_tag="VNN25_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH92599.1"
                     /translation="MDTIRVRYMITDLDPAVTFYTKHLGFQVKQQAKPHFAMLSRGPL
                     DLVLSTPFGPGGAAKPMLDGRHAEPGGWNRIIINVDDLAAEVARLRKAGIHFRSEVAT
                     GPGGSEILLDDPSGNPIELFQPAR"
     gene            3907..4137
                     /locus_tag="VNN25_13545"
     CDS             3907..4137
                     /locus_tag="VNN25_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92600.1"
                     /translation="MKKRSLSIPQLLFVAGTRAVLGAGVALLATRRLSERKRRAAGLT
                     LALVGAATTIPAARIVAGANPSLLTRLAQRFE"
     gene            4141..4587
                     /locus_tag="VNN25_13550"
     CDS             4141..4587
                     /locus_tag="VNN25_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92601.1"
                     /translation="MNGSVSLPRQGQRLLQIGAALLLFSALEGFAIPHLAAPNLALSA
                     HKLSALQGVLFLVLGLVWTKHLNLGATASRIAFWLLVYSGLAILLAYVLGAVWGAGNE
                     TMPLAGGTAHGSASQEMLIKLIAYSSASGVITFWLIFWGLRIKAVR"
BASE COUNT          953 a         1533 c         1456 g         1004 t
ORIGIN      
        1 ccgcaagttg cgctgcaagg tgatcaagat ggtcgttgcg ctcggtgagg acgaggggcg
       61 aacggccgtc atgtaccgct tcgaccacgt cgtcgcagat tcgctgattg cgaagttcat
      121 cgtcgatcaa ttcgcggtat aggctttgga acgctacgcg ctgatccgcg cccgcggtat
      181 cggcagattg aaacgccgtc ggacggacga tcaccgtatg ctcaaagggc cgcgcggtcg
      241 cctgcatctt cgcgttcacg cgatgacgga tcgggccaca ttgcatgaag aggatcggat
      301 gatggccgtc tttacgcgca acagtcgctg acaagccgac gacaaaccgg gcctttgcac
      361 tgcgggctac ttgttcgaag ctgtatgccg gcaggtgatg acattcatcc acgatcagat
      421 gcccgtactc gccaacgcgg tcgtcgacta cgcctttacg gccaaggctc tgaatgaggg
      481 cgacgtcaag cgatccgttg gctcgatcgc gcccaccgcc aatttgacca atcccgccag
      541 ccggcaaatc caaaaatgcc gacagtcgct ctacccattg gtcgagcagt tgccgccgat
      601 ggacgagaac cagcgtgttg acaccgcgct gtgcgatgag ccatgcagcg atgaccgtct
      661 tcccaaatgc cgtcgtggcg gacagcaccc cggtttcatg cgccagcatt gcgtccgcgg
      721 caatcctctg ctcgggccga agctctccac ggaacgccac gcgcagaggc agacccggca
      781 gacgctcgtc gcggacgaca ggggtgatct tcaagtctgc gagcagttgc aacagatcac
      841 cgaggcaacc gcgcggtaag ccgatgtgct gtggatgatc ttcggcgcag gcgatgatgc
      901 gcggtttgtc atacgtggcc aggcgcatcg actgcgcctt atagaactcg ggattctgaa
      961 aggccgctag ccggagcaac cggttgcgga gcgccggaac cagtccttcc ttcgcgatat
     1021 aaacctgatt cccgagaacc acctccagtg tacgtggcaa cccggcaatc gatggcagtt
     1081 tccgatagcc cgatggcggc gctgtccacg gctcttcttc gtcctcgtca gcgcgtggaa
     1141 aacggacgcc cacgatctgc ccgattcgct cggcgtcgcg aacgatgtct tcgacgcgag
     1201 accgtgtgat ccggtggact gtcgatagaa acgcccattg gtccggatag ggcgtgagtg
     1261 tctcatcaac gaaaaggctg ttgccaagct cacgcgcgcg cttctgcaac ggcagcgcga
     1321 tcagattgcc gaagcctccc cgcggcagcg tgtcctggtt cggaaagaac cgatcgtacg
     1381 aatccagacc gacttccggc cggcgctcca tcgtttcggt cagaatgtgc gcgccgagcc
     1441 tgcgcgcgag aacggctggg atggcctcct cgaagaaaag ccacacgtgt ccaccctgcc
     1501 cggagcgcga gcgttcgatc acggccggaa gattcatccg gcggcaagtt tcggcgaatg
     1561 cggacgcatc ctcccgccat cctgtcttgt cgaaatcagc agccaggaag aagcacgcct
     1621 cgtccaagag catcggatag acgcccgcga caaacggctg cccgttatcg tcacggcctg
     1681 acagatgccg ccggactaca tcgtcgttca cgggaagaaa gcgttggtga ggacagtcgg
     1741 cacacctgat ccgcggcttt tcgcagatcc ctcgcaccca ctcgttggca catgcaggcg
     1801 agtaccctga cttacccgtt ttccgactct cgaagcgctg cgcgtaaacg tcctcacgcc
     1861 cacggaagag tgcacgaaac agcgcaatct tggcctgcga cggggaccgc aagtggatac
     1921 tggtctctgt tcgatcgggt gacatgaggg atgactacca ctaagacagc acaactacgc
     1981 tctacacaag ctcaaggttg ggtttccgga agtcttcgcc gcggcagaga agtggctgac
     2041 gagccaatgc cgccgtcgcg taggtcatcc aagaatctgc tctcgctctt tcctcgttac
     2101 gcgctgtcca agaagctttc gaggaatgtt gggccaaatc tccctctcga ggaagtccag
     2161 agcctgggcc aaatctgcgc tttccagacg gcgccagggc gattctttcc ctgcgttcaa
     2221 ccggggaacg aaggatcgcg gcggttttgg attcccctgt catctgcgca agttcctcgg
     2281 ccaatcgctc caccttctcg ttcttgatat tcatcgccat ggtagaaaga ttctaccaaa
     2341 gcaccacaaa ggctgttcga atcgacgaag cactgcgtct gccttgtcgt tccagcaatc
     2401 gtgatgaccg agctcctggg aaacacgagc ctcttacagc gaaggtcagg gagtagcatc
     2461 gggattgctc aggaccactt gatgccggtc agtcgcccgt caaccatggt tccccaactt
     2521 ggataccatg cacatcaggc gaacagacta gtgcaagtag cagattcgga aggctttaaa
     2581 ttggagcggg cgacgagatt cgaactcgcg accaagagct tgggaagctc ctactctacc
     2641 aactgagcta cgcccgctcg cggttgggaa ggcgattgta catcagcagt ttcgcgggac
     2701 aacgttgaga cgcagagccc cgttagctca agagcgcgac gaaccgctgc cgcgagcagg
     2761 ccggcgatac acgcgccgta gctcgtcttt ggcttgttcc aggcgatggc ggtcgttcct
     2821 ggtgaggtgc ttgccggcgc ggttgatgta gaacgtcagc atcgacatgg ccgaacggta
     2881 agggttgctc ttgcggcggc cgctacggtc cgccgaccgc ttgagtgaac gcgcgatcgc
     2941 gcccggatcg cgcttcgaaa aaacaccttt ctcgaggtcg agggcgttgc tcgtctcggt
     3001 cacgcgctga gaccaacgcc tctgcctggt gccagctttc ggatgagctc ttgtcgttcg
     3061 caatcgatca ttcgtactgc aagttccgtg tcggtcgcga acggttcacg ctagccgcca
     3121 gtccccaagc caccttcccg cttgatcgcg ttcggccgca tgtacgctcc gcacgaggcg
     3181 cgcgcgaaga cgcggtgctc ttcccggcca ttcacatgat cgtctcgccg cacgagtacg
     3241 acgcactcgg cgaggagttc gagaagaagg agaagcgaag ccattcaggc gtcgttcgcc
     3301 ttctcgttcg tcctcatgat gggcatcatg ttcatctata gcggcggcag gctttcgttc
     3361 gacgcgctca taccgcacgg tcaacgagca ggctgaaaga gctcgatcgg gttgcccgat
     3421 ggatcgtcca acagaatctc ggatccgcca gggccggtcg cgacttcgct ccggaagtgg
     3481 atgccagcct ttcgaagtct cgcaacttcg gccgccaggt cgtcaacatt gatgatgatt
     3541 cggttccagc cgcctggttc cgcgtgacgc ccatcgagca tcggcttcgc cgctcctccg
     3601 ggcccaaacg gcgtactcaa caccaggtcc agtggccctc gagagagcat cgcgaagtgg
     3661 ggtttcgctt gctgcttgac ctgaaaaccg aggtgcttcg tgtagaaggt cacggcaggg
     3721 tcgaggtcgg tgatcatgta acgcactcgg atcgtgtcca tatccgcctc ctcgtggacc
     3781 atttgcatgg cgagagccac cgtctttcgg ccaggagctt catcgtaggc gcatgtgcct
     3841 gcttacctcg tcattctcac gatcggcgtc atgagacttg cagggaaatc gcgcaggagg
     3901 ctaacgatga agaaacgctc gctgtccatt ccgcaactcc tgttcgtggc cggtacgcgc
     3961 gcagtcctcg gtgcaggcgt tgcgcttctc gcgacccgtc gtttatccga gcggaagcgc
     4021 cgcgccgccg gactgacact cgcgctcgtg ggtgctgcga cgaccattcc cgcggctcgc
     4081 atcgttgccg gagccaaccc ttcgttgctg acacggctgg ctcaaagatt cgagtaacga
     4141 atgaacggat cggtgtcgtt acctcgtcag ggtcagcgac tcctgcagat aggcgccgcc
     4201 ctcctgttgt tttcggcatt ggaaggtttc gctattcctc atctcgctgc gccgaatctc
     4261 gcgctgtcgg cgcacaagct gagcgccttg cagggcgttc tgtttctggt tctgggactc
     4321 gtctggacca agcatctcaa tctgggcgcg accgcatcgc ggatcgcatt ctggcttctc
     4381 gtgtactcgg gtctggccat tctactcgcc tacgtgttgg gtgcagtctg gggcgccggc
     4441 aacgagacca tgcctctcgc gggaggcacg gctcacggga gcgcctcgca ggagatgctg
     4501 atcaaactca tcgcgtattc gtctgcctct ggcgtgatta ccttctggct tattttttgg
     4561 ggactgcgca tcaaagcggt gaggtgagga tcgggcgcac cgcgacgcgc ccatcctccg
     4621 gtacgaactt ctccggatga gatcgcatct gaccgcattg ccgaccgggt cgaatcgatg
     4681 ggcgatcacc cgttgtaaga tcgcgtctcc gccccgtcgc ccgctccatg gcggagaaat
     4741 ccacgtctgg tcctgaggcc acttgcgcac tgagaagccg aggttgccca cccgccgccc
     4801 gaacaatgcc gttcccctgt tcgtggcatc ggtcatggcg ctcctgatct ctacagcggc
     4861 gcaggctggc acggtcactt cggcggatgt ctcgatcgtc aagagcatca ccacacccgg
     4921 accgtactac ccgggccagt cgatca
//
LOCUS       DATLVW010000444         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_268705, whole genome shotgun sequence.
ACCESSION   DATLVW010000444 DATLVW010000000
VERSION     DATLVW010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268705"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VNN25_13555"
     CDS             <1..1479
                     /locus_tag="VNN25_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXH92602.1"
                     /translation="LAANVIGYVGLDGEGLGGIEHSFDAQVRGRAGRITLLRDARRGM
                     YQVGGEGPNRPIDGKDVYLTIDSVVQFIAERALGRVVDQYHALDGVAIVMDPRDGRIL
                     AMASNPSFDPNRFPDFPPDARKNRAVQDTYEPGSTFKIVTASAGLEEGVVTPSQIIDC
                     ADGYIQIGATQIHEHGHARYGLITFEDVMTHSSNVGAIRVGLSLGQDRFYRYIRRFGF
                     GERTGIALPGEASGRVRRTDQWSQLSNASLSIGQEIGVTPLQVLTAMATVANGGLRVQ
                     PRIIDKVTDASGNVLDVPPVGAPVRVISEKTAAVLNEILKSVVSRGTGVRAALAEHVV
                     AGKTGTAQKATRGGYSPDKVVASFAGYVPADRPRLVILVMVDEPKGEQYGGTIAAPAF
                     KEIAESTLRYLGVPPSIPARTIDVAAPMLATFSQKPLPAAGSAVPDLRGLDARAALAR
                     ATAAGFRIRASGSGVVQTQQPMPGEALPVDHQVVLTLAEGAR"
     gene            1476..>2256
                     /locus_tag="VNN25_13560"
     CDS             1476..>2256
                     /locus_tag="VNN25_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXH92603.1"
                     /translation="MKLRELLRDVPVRTSCGDLSVEIASVTADSRLAAAGSLFVAVPG
                     FQTDGARFIGSALSKGAAAVVSETTADAPVLVEVDDSRAALSTIAANFYGRPVDHLTL
                     VGVTGTSGKTTTTKMIESVFDAAGGPVGLVGTIEYRAGDERLTADRTTPDAVVLQQWL
                     AKMVEAGVHNAVMEVSSHALALKRTFGMHFSAAVFTNLTQDHFDFHKNFEDYFAAKRI
                     LFDQIDRTRKTAIVNIDDEYGRRLANELGDCVVSFGRAPKAD"
BASE COUNT          389 a          738 c          741 g          388 t
ORIGIN      
        1 ctggctgcga atgtcatcgg atacgtcggg ctcgacggcg aaggtctcgg cgggatcgag
       61 cactcgttcg acgcgcaggt tcgcgggcgc gccggccgca tcaccctgtt gcgcgatgcc
      121 cggcgcggca tgtaccaggt cggcggcgaa ggtccgaacc ggccgatcga cggcaaagac
      181 gtctacctca cgatcgactc cgtcgttcag ttcatcgccg agcgcgcgct ggggcgtgtc
      241 gtcgatcagt atcacgcgct ggatggtgtc gccatcgtca tggatccgcg cgatggccgc
      301 attctggcga tggcttccaa tccatcgttc gatccgaatc gcttccccga ctttccgccc
      361 gacgcgcgaa agaaccgcgc cgtgcaggac acctacgagc ccggctcgac attcaagatc
      421 gtgacggcgt cggcgggtct cgaagaaggc gtggtcactc cgtcgcagat catcgactgc
      481 gccgatgggt acattcagat cggcgcgacg cagatccacg agcacggcca cgcccgctac
      541 ggactcatca cgttcgagga cgtcatgacg cactcatcga acgtcggcgc catccgcgtc
      601 ggtctgtcgt tggggcagga ccggttctat cgctacatcc ggcgcttcgg cttcggtgaa
      661 cgaaccggca tcgcacttcc cggtgaggcg tcgggacgcg tgaggcgcac ggatcagtgg
      721 tcgcagcttt ccaacgcctc gctgtcgatc ggccaggaga tcggcgtgac gccgcttcag
      781 gtcctgaccg cgatggccac ggttgccaat ggcggactgc gcgtgcagcc gcgcatcatc
      841 gacaaggtca ccgacgcgag cggaaacgtg ctcgatgtgc cgcccgtggg ggcgccggtg
      901 cgcgtcatct ccgaaaagac ggccgcggtt ctgaacgaga ttctcaaatc ggtcgtcagc
      961 cgcggaacgg gtgtgcgcgc ggcgctcgcg gagcatgtgg ttgcggggaa gaccggcacc
     1021 gcgcagaagg caacgcgcgg aggctactcg ccggacaaag tggtggcttc gttcgccggt
     1081 tacgttccgg cggaccggcc gcgcctggtc atcctggtca tggtcgatga gccgaagggg
     1141 gagcagtacg gcggcacgat tgccgcgccc gccttcaaag agatcgccga gtcgactctc
     1201 cgctacctcg gcgttcctcc ctccatcccg gcgcggacga tcgatgtcgc cgctccgatg
     1261 ctggcgacgt tttcgcagaa gcctttgccg gcagcgggat cggcagtgcc tgacctgcgc
     1321 ggcctcgacg cacgagcagc gttggcgcgc gcgaccgcag caggcttccg gatccgtgcc
     1381 tcggggagcg gagtggtcca aacacaacaa cccatgcccg gcgaagctct gccggtcgat
     1441 catcaggtcg tgctcactct tgcggaggga gcgcgatgaa gctgcgcgaa ctgttgcgcg
     1501 atgttcctgt gcggacgagc tgcggggacc tcagtgtcga gatcgcaagt gttaccgccg
     1561 actcacggct cgccgccgcc ggatcgcttt tcgtcgctgt tccgggattc cagaccgacg
     1621 gcgcccgctt catcggctcg gcgctcagca aaggagcggc ggcagtcgtc agcgagacga
     1681 cggcggacgc gcctgtactc gtcgaagtcg acgattcccg tgcagcgctt tcgacgatcg
     1741 cggccaattt ctacggccgc cccgtcgacc acctcacgct cgtcggcgtc accggcacct
     1801 cgggtaaaac aacaacgacg aagatgatcg agtcggtctt cgacgccgcg ggcgggccgg
     1861 tcggactcgt cggcacgatc gaataccgcg ccggcgacga gcggctcacg gccgatcgca
     1921 cgacgcccga tgccgtcgtt ctccagcagt ggctggccaa gatggtcgag gccggcgtcc
     1981 acaacgcggt gatggaagtg tcgtcgcacg cccttgccct gaagcgaacc ttcggcatgc
     2041 atttcagcgc ggccgtcttc acgaacctga cgcaggatca cttcgacttt cacaagaact
     2101 tcgaggacta cttcgcggcg aagcggattc tgttcgatca gatcgaccgc acgcgtaaga
     2161 ccgcgatcgt caacatcgac gacgaatacg gacggcgact ggcgaacgag ctcggcgact
     2221 gtgtggtctc gtttggccgc gctccgaagg cagaca
//
LOCUS       DATLVW010000445         6749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_309517, whole genome shotgun sequence.
ACCESSION   DATLVW010000445 DATLVW010000000
VERSION     DATLVW010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309517"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="pgk"
                     /locus_tag="VNN25_13565"
     CDS             complement(<1..370)
                     /gene="pgk"
                     /locus_tag="VNN25_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXH92604.1"
                     /translation="MIRIRTLDDLTLSAGARVFYRVDYNVPLDGARITDATRIEETLT
                     TLRRLQDAGAAIVIASHLGRPKGERRAKYSLEPVRAMLAELLGAEVQWAADCIGDEAE
                     AKASQLKAGQMLLLENLRFHA"
     gene            complement(367..1392)
                     /gene="gap"
                     /locus_tag="VNN25_13570"
     CDS             complement(367..1392)
                     /gene="gap"
                     /locus_tag="VNN25_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXH92605.1"
                     /translation="MIRVGVNGFGRIGRNVLRASLERKLEIEFVAVNDLTDAATLAYL
                     LKYDSVHHTIPNDVGHTDKSITVDGKELRVFASPDPNDIPWGDLGVEIVVESSGRFTS
                     KAKAERHLRGSVEHVIISAPSKDADVTICIGVNEGVFDPAAHKIISNASCTTNCLAPV
                     AKVLHETFGIEMGIMTTVHSYTNDQEILDLPHKDVRRGRAAAMSMIPTTTGAAKAVGL
                     VLPELKGRFDGISVRVPTPNVSLVDLTFRTEKATNPEEINQVLRDAAKGPLKNILDYS
                     DEQLVSVDFNHNPHSSIIDTTFTRSVDPHMHKVLAWYDNEWGYSNRILDLIAYVGRVK
                     KDAPRSA"
     gene            1477..2460
                     /gene="mtnA"
                     /locus_tag="VNN25_13575"
     CDS             1477..2460
                     /gene="mtnA"
                     /locus_tag="VNN25_13575"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXH92606.1"
                     /translation="MHVEPIRWRGDALELLDQRLLPREVRYVALHDAHEVADAIHDMM
                     VRGAPAIGVSAAFGVALAAQRGDDVDAAAAELRKSRPTAVNLMWALDRMLRARDAGRD
                     LTAEAEAMFSEDVAACKRIGQYGAALLGNSVTVLTHCNAGALATAGYGTALGVIRAAV
                     EGGKRVAVFADETRPYLQGARLTAWELHEEGIDVTLITDSMSGHFFQEGKFDAVIVGA
                     DRIAANGDAANKIGTYTVAVLANAHGVPFYVAAPLSTIDVHCPTGAQIPIEERSAAEV
                     TSINNVSIAPEGISVRHPAFDVTPSRLITAIITDRGVLRAPYDEAIREVFR"
     gene            2457..3074
                     /locus_tag="VNN25_13580"
     CDS             2457..3074
                     /locus_tag="VNN25_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92607.1"
                     /translation="MTDEPVAEPTGEEASTVSPLAVEEETVPEAVEQPSAPVPVVEHN
                     TVPPPAGVRVSRPHPFSLAIARLVRALWLGSGAFLILTAAAAFAAASNATDAANVVGA
                     VLTRWHYIALFAPLLLLLLEWRRSRPVMLILLFIAILLASSQALLDTRIRMMRLDSLV
                     PISSLSPDDPLRRHFGFLHGMSSLFLIGQVIAAGIAVAMREERAL"
     gene            3091..4497
                     /locus_tag="VNN25_13585"
     CDS             3091..4497
                     /locus_tag="VNN25_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92608.1"
                     /translation="MDVLIVDDEASLRDFLSIVFEEEGWRVETAGSLAAARSAHAKHE
                     PDLILCDLMLPDGSGIDLLREVKASSPSVAVIMITAHTSTKSAVEALKAGAFDYIAKP
                     FDIDELKIIVRNAVERKELEDENIHLRTALEERFTFSNIIGRSARMQEIFSVVQRIAK
                     TTSTVLISGDSGTGKELIARAIHYTSGRRGKFVSINCGALPETLLESELFGHERGAFT
                     GAIREKRGLFHEAERGTIFLDEIGETSTAMQIKLLRVLQDHVVRRVGSNSETQVEVRV
                     IAATNRDLAESIRAGTFREDLFYRINVIPITLPPLRQRREDIALLVEHFITKYSKTLG
                     VPQKRISADAMRLIEKYSWPGNVRELENVIERMIALESSDVLTTKSLPEHVLLGGAIP
                     DMTFELPADGISLENHLEGIGKFFMLKALERCGGVQTQAAELLKMSFRSFRYYAKKYD
                     LIVREGRPDESMEIENEN"
     gene            4571..4978
                     /locus_tag="VNN25_13590"
     CDS             4571..4978
                     /locus_tag="VNN25_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXH92609.1"
                     /translation="MAPADAASPRIILPDKSFIAVEVAADEEMRAQGLMYRDHLADDR
                     GMIFLFPQAGEYPFWMKNTLIPLDMIWSDANHRIVHIAHDVPPCKADPCPNYPPHAEA
                     SSVLELAAGVAAKHHLAEGNTLKFEGMDNVVAR"
     gene            5066..6001
                     /locus_tag="VNN25_13595"
     CDS             5066..6001
                     /locus_tag="VNN25_13595"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXH92610.1"
                     /translation="MFRSRGENLRSTYILLFLNIAFFFLQFQDGVKFTQLFGFDRDAV
                     AAGEIWRLFTYQFAQAGHLGLFTIPPVLTLFLNLVLLSLMGFAVEAEWGTGHFLRFYL
                     MSTLATALVAAWFNTPLLGSFFINFTLLFVYAALNRDQTFSLSTILPIRITFLAWLAL
                     AALLTGVFFGSKANLAALAGAAVGFGYYISQRIRVLALVTSGAGGTAKVDSDDRAVIT
                     ATRNVTRVTAVKRAIETGSVTDIDRLIKLAEREIVSGVNICSPGDFKPEHSDRYCVRC
                     EGFAECTVRHLRMNRPNAPVPVPASASIEQAGAIT"
     gene            complement(5972..6433)
                     /locus_tag="VNN25_13600"
     CDS             complement(5972..6433)
                     /locus_tag="VNN25_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92611.1"
                     /translation="MPARPGRMGDHKSSSSSGRRAVTGVRNGLHNAADSLNVLLRRGH
                     RSSRPPRSPRGSGPDVVAGVVAVPGAERPELQRRAVHRQQRLRVRHRGHRTRVALRVR
                     RKQKRTAERRAPRVKADRSGGDVSVVAGRAVVTAVDPRAARQLRNRAGLLD"
BASE COUNT         1176 a         2171 c         2076 g         1326 t
ORIGIN      
        1 cggcgtgaaa gcgaaggttt tcgagaagca gcatctggcc cgctttcagt tgcgaggcct
       61 ttgcttccgc ctcatctcca atgcaatcag ccgcccactg aacctcggcg ccgaggagct
      121 cggccagcat cgcgcgcacc ggctcaagcg agtacttcgc tctgcgctcg ccttttgggc
      181 gtccgaggtg gctggcgatc acgatggcgg cgccggcatc ttgcagacgg cgcagtgtcg
      241 tcagcgtctc ttcgatccgc gtcgcgtcgg tgatccgagc accatcgagc ggtacgttgt
      301 aatcgacgcg atagaaaacg cgtgctccgg cggagagcgt caggtcgtcg agcgttcgga
      361 tccggatcat gcgcttcgcg gggcgtcctt cttcacccgt ccaacatagg cgatcagatc
      421 gaggatccga ttcgaatagc cccactcgtt gtcgtaccac gccagcacct tgtgcatgtg
      481 cggatcgacc gagcgggtga aggtcgtgtc gatgatcgag gagtgcgggt tgtggttgaa
      541 gtcgacggaa accaattgct cgtcggagta atcgaggatg ttcttgagcg gccccttcgc
      601 ggcgtcgcgg agcacctgat tgatctcctc ggggttcgtt gctttctccg tccggaacgt
      661 cagatcgacc agcgaaacgt tcggcgtcgg cacgcgcacc gagataccgt cgaagcgacc
      721 cttcagctcc ggcaggacca agccgaccgc tttggccgcg ccggtcgtgg taggaatcat
      781 cgacatcgcc gccgcacgcc cgcgacgcac gtccttgtgg ggcagatcga gaatctcctg
      841 gtcgttcgtg tacgagtgga ccgtggtcat gatccccatc tcgatcccga acgtttcgtg
      901 gagcaccttc gccacaggcg cgagacagtt cgtcgtgcag gaggcgttcg aaatgatctt
      961 gtgcgcagcc ggatcgaaga cgccttcgtt gacgccgatg cagatcgtga cgtcggcatc
     1021 ttttgaaggg gcggagatga tgacgtgctc gacggagccg cgaagatggc gttcggcctt
     1081 ggccttcgac gtgaagcggc ccgacgactc gaccacgatc tcgacgccga ggtcgcccca
     1141 gggaatgtcg ttgggatcgg gagacgcgaa gacgcgaagc tcttttccat cgacggtgat
     1201 gcttttgtcg gtgtggccga catcgttcgg aatcgtgtga tgcacggagt cgtacttaag
     1261 cagatacgct agcgtggccg cgtcggtgag atcgttgacg gccacgaatt cgatttcgag
     1321 tttccgttcg agcgaggcgc gcagcacatt ccgcccgatc cgcccgaaac cattcactcc
     1381 taccctgatc attgattctc caagtgtttt ctgagtccgc acacagtggt gtctgactcg
     1441 gcgcgtagta taacgacgcg tttcactgcg aagcccatgc acgttgaacc tatccgttgg
     1501 cgcggcgacg cgctcgagtt gctcgatcag cgccttttgc cgcgagaagt acgctacgtc
     1561 gcgttgcacg atgcgcacga ggtcgccgac gccatccacg acatgatggt ccgcggcgca
     1621 ccggccatcg gcgtatcggc cgcgtttggc gtcgcgctgg cagcacagcg cggcgacgat
     1681 gtcgatgccg cggcagccga gttgcgcaaa tcacgtccca cagccgtcaa cctgatgtgg
     1741 gccctcgatc gcatgctgcg cgcacgcgat gccggacgcg atctgaccgc cgaggcggag
     1801 gcgatgttca gcgaggatgt ggcggcctgc aaacgtatcg gccaatacgg cgccgcgctc
     1861 ctcggcaatt ccgtgacggt actgacccat tgcaacgccg gcgcactggc cacggccggt
     1921 tacggcaccg cgctgggcgt gattcgtgcg gccgtcgagg gtggcaagcg ggtggcggtc
     1981 ttcgccgacg aaacgcggcc gtatctgcag ggcgcgcggc tgaccgcgtg ggaactgcac
     2041 gaggaaggga tcgacgtcac gctcatcacc gacagcatgt cgggtcactt tttccaggaa
     2101 ggcaagttcg atgcggtgat cgtcggcgct gaccgcatcg ccgccaacgg cgacgcagcg
     2161 aacaagatcg gaacgtacac ggtggccgtt ctggccaacg cgcacggagt gccgttttac
     2221 gtcgccgctc cgttatcgac gatcgatgtg cattgcccga ccggcgcgca gatcccgatc
     2281 gaggaacgga gcgccgcaga agtgacgtcg atcaacaacg tctcgatcgc gccggaaggc
     2341 atcagcgtgc ggcacccggc attcgatgtc acgccgtcgc ggctgatcac agccatcatt
     2401 accgaccgcg gcgtgctgcg tgcgccgtac gatgaagcga tccgcgaggt gtttcgatga
     2461 ccgatgagcc ggttgcagag ccgacaggcg aagaagcatc caccgtctcg ccgttggccg
     2521 ttgaggagga gacggtaccc gaggctgtcg aacaaccgag tgcgcccgtc cccgttgtcg
     2581 aacacaatac cgttccaccg cccgccggag tccgcgtctc ccgaccacac cccttctcgc
     2641 tcgccatcgc gcggctcgtt cgcgcgttgt ggctcggcag cggcgcattc ctcatcctca
     2701 ccgccgccgc ggcattcgct gcagcgagca atgcgaccga cgccgcgaat gtcgtcggag
     2761 ccgttctcac gcgctggcac tacatcgccc tctttgcgcc gcttctgctc ctgctgctgg
     2821 agtggcgccg cagccgtccg gtcatgctca tcctcctctt catcgccatc cttctggcgt
     2881 cctcgcaggc gctgctcgac acccgcatcc gtatgatgcg tctcgactcc ctggtgccga
     2941 tcagcagcct ctcgcccgac gatccgttgc gacggcactt cggattcctg cacggaatgt
     3001 cgtcgctctt cctgatcggt caggtcatcg ccgcgggcat cgcggtcgcg atgcgcgagg
     3061 agcgagccct ataatcgccg cggtgaaatc gtggacgtac tgatcgttga cgacgaggcg
     3121 tcgctgcgcg acttcctctc gatcgttttt gaagaagagg gctggcgcgt cgaaacggca
     3181 ggctcacttg ccgctgcgcg cagcgcgcac gcgaagcacg agccagatct catcctttgc
     3241 gacctcatgc tgccggatgg ctccggcatc gacctgctgc gcgaggtgaa agcgtcgtct
     3301 ccttcggtgg cggtcatcat gatcactgct cacacgtcga cgaagtcggc ggtcgaggcg
     3361 ctcaaagccg gcgccttcga ctacatcgcc aaaccgttcg atatcgacga gctgaagatc
     3421 atcgtccgca acgccgtcga gcgaaaagag ctcgaggacg agaacatcca cctgcgcacg
     3481 gcgctggaag agcgattcac gttctccaac atcatcgggc gcagcgcacg gatgcaggag
     3541 atcttttcag tcgtgcagcg gatcgccaag accacgtcga ccgtgttgat ctccggcgac
     3601 tccggcaccg gcaaagagct gatcgcccgg gccattcatt acacctcggg gcgccgagga
     3661 aagttcgttt cgatcaactg tggtgcgctg cccgagacgc tgctggagtc ggagctgttc
     3721 ggccacgagc gtggcgcgtt caccggcgcg attcgcgaga agcgcgggct gtttcacgag
     3781 gcggagcgcg gcacgatctt tctcgatgag atcggcgaga cgtcgaccgc gatgcagatc
     3841 aagctgctgc gcgttcttca ggatcacgtc gtgcggcgcg tcggttcgaa cagcgagacg
     3901 caggtcgaag tccgcgtcat cgcggcgacg aaccgcgacc ttgcggagtc gatccgcgcc
     3961 ggtaccttcc gcgaggatct cttctatcga atcaacgtca tccccatcac actccctcca
     4021 ctgcgccagc gcagggagga catcgctctg ctcgtcgagc acttcatcac caagtactcg
     4081 aagacactgg gcgtccctca aaaacggatt tcggcggatg cgatgcggct gatcgagaag
     4141 tacagttggc ccggcaatgt gcgtgagctg gagaatgtca tcgagcggat gatcgcgctg
     4201 gagtcgtcgg acgttctcac gacgaagtct ctcccagagc acgtcctcct cggtggcgcc
     4261 attcctgaca tgacgttcga actgccggcc gatgggatct cactcgaaaa ccatctcgag
     4321 ggcatcggta agttcttcat gctcaaagcc ctcgagcgct gcggtggcgt gcagacccag
     4381 gccgccgaac tgctgaagat gtccttccgc tcattccgct actacgccaa gaagtacgat
     4441 ctcatcgtac gggaagggcg tccggatgag tccatggaga tcgaaaatga gaattagctt
     4501 agctctgctt ctcgcatgcg cgcttgcctg tgcgcaacgg gtgccgccac cgacgccgtc
     4561 gtcagcggct atggcccctg cggacgcggc ctctccgcga atcatccttc cggacaaatc
     4621 cttcatagcg gtcgaagtcg cggcggatga agaaatgcgc gcccagggtc tgatgtaccg
     4681 ggatcatctc gccgatgacc gcggaatgat ttttctcttc ccgcaagcgg gcgagtatcc
     4741 attctggatg aagaacacgc tcattccgct cgacatgatc tggagcgacg ccaaccatcg
     4801 catcgtgcac atcgcgcatg acgtcccgcc gtgcaaggcc gacccctgcc cgaactaccc
     4861 ccctcacgcc gaggcgtcgt cggtcctgga gctggcagcg ggggtggcag cgaagcatca
     4921 cctcgccgaa ggaaacacgc tgaagttcga aggcatggac aacgtagtcg cccggtaaac
     4981 agttcaacac ctccccagtc tgattcgtat caaatcccgc cgggagtgtt gaactattca
     5041 atcgcgaggg tatgttcgga ccgtcatgtt ccgcagccgc ggggagaatc tgcgctcgac
     5101 gtacattctg ctgttcctga atatcgcctt cttctttctg cagtttcagg acggtgtgaa
     5161 gttcacgcag ctttttggat tcgatcgtga cgccgtcgcc gccggagaga tctggcgcct
     5221 cttcacctac cagttcgcgc aggccggaca cctcggcctc ttcacgattc cgcccgtcct
     5281 gacactcttt ctcaatctcg tgctgctttc tctcatgggg ttcgcagtgg aagcggagtg
     5341 ggggaccgga cattttcttc gtttctattt gatgtcgacg ctcgccacag cactggtggc
     5401 ggcatggttc aacacgccgc ttctcggctc gttcttcatc aacttcacac ttctgttcgt
     5461 ctacgcggct ctgaaccgcg accagacctt ttccctatcg acgattcttc cgattcgcat
     5521 cacctttctg gcatggctgg ccctcgctgc gctgctgacc ggcgtcttct tcggcagcaa
     5581 agcaaacctt gctgcacttg cgggtgcagc ggtgggattc ggctattaca tctcgcagcg
     5641 gatccgcgtt ctcgcgctcg tcacgagtgg cgctggcgga acagccaagg tcgattccga
     5701 tgaccgagca gtgatcacgg cgacgcgcaa cgtcacgcga gtgacggctg tcaagagggc
     5761 gatcgagacc ggatcggtga ccgacatcga tcgattgatc aagctcgccg agcgggaaat
     5821 cgtcagcggc gtgaacatct gttctccggg cgacttcaag ccggagcatt cagaccgcta
     5881 ctgcgttcga tgcgaaggct ttgcggaatg cacggtgcgc cacctccgca tgaaccggcc
     5941 gaacgcaccg gtgccggtac ccgcctccgc ctcaatcgag caagccggcg cgattacgta
     6001 gctggcgggc cgctctgggg tccaccgccg tcaccaccgc ccggccggcg acgacggaaa
     6061 cgtctccgcc gcttcgatcc gccttcaccc tcggggcgcg gcgctccgcc gtccgctttt
     6121 gcttgcggcg gacgcggagg gcgaccctcg tgcggtggcc tctgtggcgg acgcggagtc
     6181 gctgctgccg gtgcaccgcc cgccgttgga gctccggcct ctccgcgccc ggcaccgcga
     6241 cgacgcctgc gacgacgtcc ggtccgctcc ccctcgggct gcgcggcggc ctgctgctgc
     6301 ggtggccgcg cctgaggagg acgttgaggc tgtccgccgc gttgtggagg ccgttgcgga
     6361 cgcccgtcac ggcccttctc ccgctgctgc tgctgctttt gtggtctccc atccggcctg
     6421 ggcgggcggg caccgccgcc gtctcgccgc gcgtctttcc ggcccgtccg ctgctcgaac
     6481 tcctgcagct tcttctgcat gaacttccgg atcggcccgt cctgccgcgc acgcacatcg
     6541 tcgaaaccgc agtagggaca gcgcttccag ccttcggggt acatcttcct gcaggagctg
     6601 caaagaatac ttttgggcag cggcatggtc gggcgggatt gtacacttcc ggccgtggac
     6661 gccaggaacg atgcggagcg gcgcaaaaca ctgctgacgt tgatcgagtt agcggaaggc
     6721 tcgagcccgt ccgattgctg ggacgatgc
//
LOCUS       DATLVW010000446         5275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_18520, whole genome shotgun sequence.
ACCESSION   DATLVW010000446 DATLVW010000000
VERSION     DATLVW010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18520"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNN25_13605"
     CDS             complement(<1..283)
                     /locus_tag="VNN25_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92612.1"
                     /translation="MKDVLKQLARDLKAEKLRTFLTIFGIVWGTVAISLMLAFGTGLH
                     TQLIKSTAGLGDRIVIAWPGLTSIPFEGLGKGRKVRITDDDLATARGNVP"
     gene            complement(490..1581)
                     /locus_tag="VNN25_13610"
     CDS             complement(490..1581)
                     /locus_tag="VNN25_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH92613.1"
                     /translation="MRKLWRTLFILVVLAGASAGVYAWTRSGKKDDGLKQVEVIQGSI
                     VEKAVAVGQIQPRQKFAVKSKISGIVKRALAQVGDTVQAGDPLFEIAPDPTPMEVTEV
                     DRQVESASASYRKAEGDYQRSLQLSRSGVLPKSDVEAKKESYDLARVAMTKAEQNRDL
                     TRKGRLTVSGTESIIRAPAAGTILTRTVNPGDPIVPLTSYQPGTEMATVADMRDLIFK
                     GTVDEIDVGKLSVGMQTRIKVGALPTDVVTGHVSRIAPQAQQKEGATLFDVEVELEPS
                     KITLRAGYSANADVIIREHKDVLVIPERLVTFGDGGKTASVEVPGSDPKSPKKVNIKT
                     GISDGLNVEVLSGLKKGEKVVERPPKEIS"
     gene            complement(2248..3276)
                     /locus_tag="VNN25_13615"
     CDS             complement(2248..3276)
                     /locus_tag="VNN25_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH92614.1"
                     /translation="MRHLTALALLLFAFAVHAQAPAPVEADFLVKDFHFASGEVLPEA
                     RIHYATIGTLRKTAHGSNAVLVLHGTGGSLKQFLNDHFAGVLFAPGGLLDANRYFIVI
                     PDNIGHGKSSKPGDGLRAKFPHYGYNDMVELQHRLLSEGLGIDHLAVVMGTSMGGMQT
                     WMWGERWPDAADALVPLASAPAQIAGRNRIWRKMMIDDLQRNDLAAALQILILVGSSP
                     LQWQKAAPTRDDADRWIAEQMKSRTATNDADDLLYAIESSRDYDPAPQLDHITAPLLA
                     INSADDFINPPELGIMESAIKRVKHGRYLLIPISDATHGHGTHTWAPVWEKQLREFLE
                     ATPAAAWR"
     gene            complement(3629..3874)
                     /locus_tag="VNN25_13620"
     CDS             complement(3629..3874)
                     /locus_tag="VNN25_13620"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH92615.1"
                     /translation="MTERAQAVLREAMSLPADERADVAAELLASLDDDAPEDPAAVQA
                     AWTKEIERRARRVLAGESSGQPWDDVRDRVARRLKQH"
     gene            3969..4784
                     /locus_tag="VNN25_13625"
     CDS             3969..4784
                     /locus_tag="VNN25_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92616.1"
                     /translation="MIPTIFAVVLFFATSSASWYEHYERGVRFIEQGNAAAARTELEA
                     ALAAHPAEGLQLATAPRQYTDYLPHLYLAIADQMSGDVAAARKQLALAEDSGVAIRSE
                     AGRPLLVAYQLLLRGDSTGKFALPAYAVYAERPPILSEAEFNTLRNDVLNKCNLPDAK
                     LSDAPWYARYELALELEQKGDYPRALRQLIDAVALRPNPQRHARMYGMWLIDYYPYFH
                     IAREHVRLENWECARNALEISQRLSEIPAGAPEINELMAMQQETARKLAGAGK"
     gene            4967..5233
                     /locus_tag="VNN25_13630"
     CDS             4967..5233
                     /locus_tag="VNN25_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HXH92617.1"
                     /translation="MDYSLWEPVVLNRDLPEHGLRIGDLGTIVEIYEPDGIEVEFVTA
                     SGRTGALLTLNVSDVRHVADADLVTVRQFARSAGRPHRARQLPS"
BASE COUNT         1011 a         1622 c         1623 g         1019 t
ORIGIN      
        1 ccggaacatt gccgcgcgcg gtggcaaggt cgtcgtcggt gatgcggact tttcgtccct
       61 tgccgagccc ttcgaatggg atcgaggtaa ggcccggcca ggcgatgacg atgcggtcgc
      121 cgagaccggc agtcgacttg atgagctgcg tgtgcagtcc tgtgccgaag gcgagcatca
      181 ggctgatggc gaccgttccc cagacgatgc caaagatcgt gaggaaggtc cgcagcttct
      241 cagccttgag gtcgcgggca agctgtttta gtacgtcttt catgattgcg cgtggcgtga
      301 gttggttgtc ggttgtcggt tagttgcgct tcgcgcgtcg tcagttactc acgttccgtt
      361 ctgtttcttt ccaacgttcc tggccgatgc agggcgagtg cgtctcgcat gtaccgcgtt
      421 gctaactgac aacgcgcgaa gcgcaaccaa ccgacaaccg acaacgcgcg gcccgccagg
      481 gccgcgcaac taagaaattt ccttcggcgg tctctccacg accttctcgc ccttcttcag
      541 gccggacagc acttcgacat tcaggccgtc gctgatgccg gtcttgatgt tgaccttctt
      601 cggagacttc ggatcgctgc cgggcacttc gacgctggct gtcttgccac cgtcgccgaa
      661 cgtgacgagc cgctccggga tgacgagtac gtctttgtgc tcgcggatga tgacgtcggc
      721 gtttgccgag tagccggcgc gaagcgtgat cttgctcggc tcgagctcga cctcgacgtc
      781 gaagagcgtt gctccctcct tctgctgcgc ctgcggagcg atgcgcgaga cgtggccggt
      841 gacgacgtcg gtggggagcg caccgacttt gatgcgcgtc tgcatgccga cggagagctt
      901 gcctacatcg atctcatcaa ccgtaccctt gaaaatgaga tcgcgcatgt cggcgacggt
      961 ggccatctcg gtgccgggct ggtacgaggt gaggggaacg atcggatcgc ccggattgac
     1021 agtgcgggtg agaatcgtgc cggccgccgg cgcgcggatg atcgattcgg tcccggagac
     1081 ggtcagtcgc cctttgcgcg tgaggtcgcg gttctgctcg gccttcgtca tcgctacgcg
     1141 cgcaaggtcg tacgattctt tcttcgcttc gacgtcggat ttcggcagca cgccggagcg
     1201 cgagagttgc agcgagcgct ggtagtcgcc ttccgctttc cgatacgacg ccgaggccga
     1261 ttcaacctgc ctgtcgacct cggttacctc catcggcgtc ggatcaggcg cgatctcgaa
     1321 aagaggatcg ccggcctgca ccgtgtcccc gacctgcgcc agggcacgtt tgacgatgcc
     1381 cgatattttc gatttgaccg cgaacttctg ccgcggctgg atctgcccga cggcgaccgc
     1441 cttttcgacg atcgatccct ggatgacttc gacctgcttg aggccgtcgt ctttctttcc
     1501 gctgcgcgtc caggcataca cgcccgcgct cgcgcccgcc agcacgacca gtatgaacaa
     1561 cgttctccat agcttccgca taggtgatct ccgagctccg aagggcttgt acggaggttt
     1621 ggacgtttgg ttaacgatga agttgggtgc ggaacgcggc gtaccagcct ttatctgcgt
     1681 ccatctgcga gatctgcgga tcaatcaggt ggggaccgat ccgcagatga cgcagatgga
     1741 cgcagatcga tgcattcggt aagttttacc gtgacgcatt ccaaaataga tgcttccgac
     1801 acacaaatct tgagtccgga ctcaagaaac gaaggtccgg accctagaaa ctaaggtccg
     1861 gacccaagaa actaaggtcg ggaccctaga actaaggtcc ggacccaaga aactaggtcg
     1921 ggaccttaga aacgaaggtc cggaccaaga aactaaggtc gggaccctag aactaaggtc
     1981 cggacccaag aaactaaggt cgggacctta gaacgaaggt ccggactcaa gaaactaagg
     2041 tcgggacctt ggaacgaagg tccggactca agaaactaag gtcgggacct tgaaacgaag
     2101 gtccggactc aagaaactaa ggtcgggacc ttagaaacga aggtccggac tcaagaaact
     2161 aggtcgggac cttggaaacg aaggtccaga ctcaagaact aaggtccgga ctcaagaaac
     2221 ttgagtcggg ggactccaga aacgacgtca ccgccaagct gcggccggcg tggcctccag
     2281 gaactcccgc aactgcttct cccacacagg agcccacgtg tgcgtgccat gcccgtgggt
     2341 ggcgtcgctg atcggtatta gcaggtagcg tccgtgcttc acgcgtttga tcgcgctctc
     2401 catgatgccg agctcgggcg ggttgatgaa gtcgtccgct gagttgatgg ccagcagcgg
     2461 tgcggtgatg tgatcgagct gcggagccgg gtcatagtcg cgcgacgact cgatggcgta
     2521 gagcagatcg tcggcgtcgt tcgtagcggt ccgagacttc atctgctcgg cgatccagcg
     2581 gtcggcgtca tcgcgggtag gcgccgcttt ctgccattgc agcggactgc tgccgacgag
     2641 gatcagaatc tgcaacgcgg cagcgaggtc gttgcgctgc agatcgtcga tcatcatctt
     2701 ccgccagatg cgattgcgcc ccgcgatctg cgccggtgca cttgccaacg gcacgagcgc
     2761 gtcggcggcg tccggccagc gctctcccca catccacgtc tgcatcccgc ccatcgacgt
     2821 ccccatgacc actgccagat gatcgatgcc gagcccctcg gacagcagcc gatgctgcag
     2881 ctcgaccatg tcgttgtagc cgtagtgagg aaacttcgcg cgcaatccat cgcccggctt
     2941 gctcgacttc ccgtgaccga tgttgtcggg gatgacgatg aagtaccgat tggcatcgag
     3001 cagcccaccc ggcgcgaata gcacgcccgc gaagtggtca ttgagaaact gcttgagtga
     3061 tccgcccgtt ccgtgcagca cgagcacagc gttggaccca tgcgccgtct tccgcaacgt
     3121 cccgatcgtc gcatagtgaa tcctcgcctc aggcaacact tcgccgctgg caaagtgaaa
     3181 atccttgacg agaaagtcgg cttcgaccgg cgcaggcgct tgtgcgtgaa cggcgaaggc
     3241 gaaaagaagg agagcgagag cggtgaggtg gcgcatgggc gaagtgtagc ccggggatgt
     3301 tcttgcgctc caagctattg gatgactcgc gtgtgccagt acccgggcct tctccgatcg
     3361 tgcgcaaacg cgagaatccg aatcgcgtcg gtcatctcga gatacacgat ttgataagga
     3421 aaccgccgga tcggtacacg acgcacagta gcatccactg gtacatcagg aactggagtt
     3481 cccgtatgag gccaatgagc gaggaaggca agcgcatcat cgaccgcttc catgaattca
     3541 ccgccgagcc caggccgttg ttgctcgtac cacacggccg cctcctcgag ttcctcggca
     3601 gcttcgcttt cgatacggaa tggaaggatc aatgctgttt gaggcggcgc gccactcggt
     3661 cgcgcacgtc gtcccacggc tgacccgacg attcaccggc cagcactcgg cgggcgcgcc
     3721 tttcgatttc cttcgtccac gcagcttgaa ccgcggccgg atcctcgggc gcgtcgtcgt
     3781 caaggctggc cagtaattcc gcggcgacat cggctcgctc gtcggctggc agcgacatcg
     3841 cctcgcgcag gacggcttgt gctcgttcgg tcattgtatt gattgtagcc gctggcccac
     3901 agcgccaaca agccagcctt cgccggacgc gggttagaat ctcccattcc caaccttgga
     3961 gacccacgat gatcccaacg atctttgcgg tcgtactctt cttcgctacg tcttcggcta
     4021 gctggtacga acactacgag cgcggcgttc gtttcatcga gcagggaaat gccgctgccg
     4081 cgcgtacgga gctcgaggcg gcgctggcgg cgcatcccgc ggaagggttg cagctcgcca
     4141 cggcgccgcg gcagtacacc gactatctcc ctcatttgta tctcgcgatc gccgatcaga
     4201 tgtcgggcga tgtcgcggcg gcgcggaagc aactcgcgct cgccgaggac agcggcgtgg
     4261 cgatcaggag cgaggcggga cggccgctgc tcgtcgcgta tcaactgctg ctccgcggcg
     4321 acagcacagg caagttcgcc ctgcctgcat acgccgtcta cgccgagcgg ccgccgattc
     4381 tcagcgaggc ggagttcaac acgctgcgca acgacgtcct gaacaaatgc aatctgcccg
     4441 acgcgaagct ctccgatgcg ccttggtacg cgcgttatga gctggcgctg gagctcgagc
     4501 agaagggcga ctatccccgc gctctccggc agctcatcga cgccgtcgcg ctccgcccga
     4561 acccgcaacg ccacgcccgc atgtacggaa tgtggctgat cgactactac ccctactttc
     4621 acatcgcccg cgagcacgtc cgcctcgaga actgggagtg cgcccgcaac gctctcgaaa
     4681 tctcacaacg cctgtccgag atcccggccg gcgcgcccga gatcaacgag ctgatggcga
     4741 tgcagcagga gacggcaagg aagttggcgg gggcggggaa gtgacgcatc gggtaaagat
     4801 ccagacgccc gcgtcgaacc ggagagagac tgaggttcca gtcgtcgctc gcggagaggc
     4861 gcgggcgtac aaccttgacg agcacctcgc cagagttcag cgctcgaatc tacatgaaga
     4921 gaccgattga ggtcaccggt cagtaaggaa gtatggtgat cgcgtgatgg attactcctt
     4981 gtgggaaccc gttgttctca atcgcgatct ccctgagcat gggctcagga tcggcgacct
     5041 cggcacgatc gtcgaaatct acgagccgga cggcatcgag gtcgaattcg ttacggcgtc
     5101 tggtcgaacc ggcgctttgc tcacgctgaa tgttagcgac gtgcgccacg ttgccgacgc
     5161 cgatctcgtc acggtccgac agttcgcacg ctccgcgggt cgccctcatc gcgcgagaca
     5221 gcttccctca tgaagctccg ttgctccgac gttcaggcgc gccatcagcc gctct
//
LOCUS       DATLVW010000447         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_554968, whole genome shotgun sequence.
ACCESSION   DATLVW010000447 DATLVW010000000
VERSION     DATLVW010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554968"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VNN25_13635"
     CDS             <1..261
                     /locus_tag="VNN25_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92618.1"
                     /translation="FFDLQHPSKPNRTRSASIDAAGNIRLGNGAKLAGQTPKNVLGLE
                     VVVPPSGGAPYMGTTHAADGGFTQQYLQKDGSFADFHFSFTA"
     gene            289..558
                     /locus_tag="VNN25_13640"
     CDS             289..558
                     /locus_tag="VNN25_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92619.1"
                     /translation="MDSALAPILAATGIVGLFSAASVLVIQLFRENGRVRDERGHEIK
                     DLKNDVAALKAENLACKFQVNALVNMLRSNGIDLPDWLLRDGGHA"
     gene            542..883
                     /locus_tag="VNN25_13645"
     CDS             542..883
                     /locus_tag="VNN25_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92620.1"
                     /translation="MVDMHSGGKSVALLVIIAVLTVATLAGVASMYGAVNSAKNNTAL
                     LRYNSKRNDCIRDVQADAEQQFRKDIADLLAAGRDQTKYGPLVVRMQQEAKVNYAQTV
                     KKVCPPALKGS"
     gene            883..1167
                     /locus_tag="VNN25_13650"
     CDS             883..1167
                     /locus_tag="VNN25_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92621.1"
                     /translation="MSAVLVAVIVGAFAFVGPLILKRQDYKRQDAVADRVKEAAELLA
                     TTSTVTQEKLDEIHVLVNSRLTAALDEIAALRAYLDGITRPGDPELPEPH"
     gene            1241..1591
                     /locus_tag="VNN25_13655"
     CDS             1241..1591
                     /locus_tag="VNN25_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92622.1"
                     /translation="MNFTSFTPLILLGTALYTFQNLIRFGKAKDWNGALSIVLSCLAG
                     IAAIALAAHSAATGSLVLVQGGSPLGALDGGAQVLLGIAVGTTAPVLADTFKAFDGNR
                     NSAKPPLVGNDPPS"
BASE COUNT          432 a          711 c          665 g          408 t
ORIGIN      
        1 ttcttcgacc tccagcaccc gtcgaagccg aaccggacgc ggagtgcatc gatcgacgcg
       61 gccgggaaca tccgattggg taacggggcg aagctcgccg gccagacacc gaagaacgtg
      121 ctcgggctgg aagtggttgt ccctccttcg ggtggcgcgc catacatggg cactacccac
      181 gcggcggatg gcgggttcac gcagcagtat ctccagaagg acgggtcctt cgctgacttt
      241 cacttctcat tcactgcgta agtccaccga ggacgggcgg cgacaccgat ggacagcgcg
      301 ctggccccaa tcctcgccgc gacgggaatt gttggcttgt tctcggcagc gtcggtgctc
      361 gtcatccaac tgttccggga gaacggccga gtacgcgatg aacggggcca cgaaatcaaa
      421 gacctcaaga acgatgtcgc cgcactgaaa gcagagaacc tcgcctgcaa gttccaggtg
      481 aacgcgctgg tgaacatgct ccgctcgaat ggcatcgatc tgcctgactg gctcttgcgg
      541 gatggtggac atgcatagcg gcggcaaatc agtagcgctc ctcgtcatca tcgcagtcct
      601 cacagttgcg acgcttgcgg gtgttgcgtc gatgtacggc gcggtgaact ccgcgaagaa
      661 caacactgca ctccttcgct ataactcgaa gcgcaacgac tgcatccgtg acgtgcaagc
      721 ggacgccgag cagcagttcc gcaaagacat cgccgatctt ctcgccgcag gacgtgacca
      781 gacgaaatat gggcccctcg tcgtgcggat gcagcaagaa gcgaaggtga actacgcgca
      841 gacggtgaag aaggtgtgcc cgccggccct caagggttcg tgatgtccgc ggtcctcgta
      901 gcggtgatcg tcggagcctt cgccttcgtc ggcccgctga tactgaaacg tcaggactac
      961 aaacgtcaag acgccgtagc cgaccgagtg aaggaagccg ccgaactcct ggcaacgacc
     1021 tcgacggtga cgcaggagaa gctcgacgag attcacgtcc tcgtcaactc acggctcacc
     1081 gccgcgctcg acgagatcgc tgcgctgcgc gcgtacctcg atggcatcac ccggcccggc
     1141 gacccggaac tccccgaacc gcactaggag ccgtcttgct tgttctcatc ctcatcattc
     1201 tcgcgatcct cgcgctcatc ctctacatag tgaggcatta atgaacttca cgtccttcac
     1261 cccactcatc ctgctcggga cggcgctcta cacgttccag aacctcatcc gcttcgggaa
     1321 ggcgaaggac tggaacggcg cactgtcgat tgtcctctcc tgtcttgcgg ggattgccgc
     1381 gatcgcactc gccgcccact ccgcagcgac aggttcgctg gtgcttgttc agggtggttc
     1441 gccgctcggg gcgctcgatg gtggcgcaca agtcctactc ggcatcgcgg tgggcacgac
     1501 tgcgccagtc ctcgctgaca ccttcaaggc gttcgacggg aaccggaact cggccaagcc
     1561 gccactcgtc ggtaacgatc cgcctagctg atgagtcggg ccgaacggct cgcatatcgg
     1621 cggcttcggt tgcaccagtt cgacttcatg ctcgggttca tctccggcat cgtcctgatg
     1681 ctgacgctcc tgagatgagc taccgcgtgc gggaggttct ccaagcacgg agttaccgac
     1741 cggcacgcaa aaccgccgag gctcacggtc ctcatccgtc tctcgtagac gtgttcgaac
     1801 gtcatcctga atggcgacga cgactcgaag ctctgcttcg cgctgggata ccgcgagaac
     1861 cgctgccggt gcgctgcggc tcgggaaaac atcgcatcga ctgctgggac gacttctact
     1921 ggggctcgtc cggtcggcat tgtcgagagt gtgagcgtga acggctcgag gtcatttacg
     1981 cccgaaagcg taagcgcaag aactagttca gtccgcagtg ccgtcccgta taccgaagga
     2041 agcgctgatg cagcaagaca ccatcaactg gcacgcgcat gcgcgcgtcg acaagttcga
     2101 ccttgcgcag atcgcggcct gtcagcgcgc gctcgggctc gaccgggaac cgaccgggcc
     2161 ggagctactc gagttctgca ccgccgcgga cttcatcgag gcggacggca accttc
//
LOCUS       DATLVW010000448         7791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_228435, whole genome shotgun sequence.
ACCESSION   DATLVW010000448 DATLVW010000000
VERSION     DATLVW010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228435"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VNN25_13660"
     CDS             complement(<1..598)
                     /locus_tag="VNN25_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92623.1"
                     /translation="MKKMISLIGVLSVAVGLSAQSQTASMAPAQVTPSVAKPVDPVEV
                     IADAFRTHVIVALGNIEFAGNEQGHAFQVSLLRDPRISSVVDDIVVEFGDARYQDVID
                     RFTRGEDVAYASLRHVWQDTTQVEFIWDLPIYEDFFRAVRQVNASLPRAHRLRVLLAD
                     PPIDWATVRSREDVQKYALQRDAYAVDLIRREVLARKHR"
     gene            complement(982..1566)
                     /locus_tag="VNN25_13665"
     CDS             complement(982..1566)
                     /locus_tag="VNN25_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXH92624.1"
                     /translation="MLDGNVLVLNRVFQAVQITSARRALTLLYKGQVKAVDSEYRTYD
                     FDNWADIPVQADDHYVRTPSRNILIPHVVQLLIFDRLPRQEVKFSRGNIYLRDGNRCQ
                     YCGKKFSSSELSLDHVVPISRGGKSTWENVVCACLPCNVKKGNKLLTETGLQIKRQPV
                     RPRWHPLHRLQGRNFPDIWKNFLDEAYWNVELTE"
     gene            1847..3355
                     /locus_tag="VNN25_13670"
     CDS             1847..3355
                     /locus_tag="VNN25_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92625.1"
                     /translation="MRVLSRIALVVLVAAALVPAAAFADQYQASCPLTLVATNAAPTA
                     FAISPHGVFRFGSQVFALRGQTLTTYTVTDLGDMQIAREDFIGALAARETKGGVAFAN
                     GFLYVSSEAGLEIYDLRSVRAGGTAPILISRTQGLRYHRLAINGNILAALYPATDLPC
                     SANGPTTPNCFNAIDIYDVSNPLVPTRVSSLSTSLSPLGGYNDIAFNFGFLIAAANTN
                     TVAWNLTSPSAPTLVGSLAVPGTFLVSNGSNLLGVGNNNVIITYKIVPSSVSGNFFVP
                     FTYHSTANLQVERANPIIFHPQAVFDEANERLITMIDELDYQTLQPARTIAFDVFDYA
                     TAMLEGRDPRQYEQVSYTQGDEVKWNPLSVGSLVYVVGEMTGTQTYGDCPLMSGRIEF
                     DTINGTVCGGSYIHGWVTGTLKITNVELFLDGGSLGSASLAGAPRNDIPSTTPVFTWQ
                     IAANLDATTKGVHVLRAVGTDVNGNRRQFSSQPILFPGPGSNCVARRKAASH"
     gene            complement(3347..4036)
                     /gene="pyrF"
                     /locus_tag="VNN25_13675"
     CDS             complement(3347..4036)
                     /gene="pyrF"
                     /locus_tag="VNN25_13675"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXH92626.1"
                     /translation="MQATDRLSNRLIVAIDRSSRDEILRLADALHGSVGLLKIGLQAF
                     VSNGPSIVREVQERGAKVFLDLKIHDIPNTAQHAVAAAAALGAEMVTVHASGGTAMLR
                     ACAVDTTLVLGVTILTSLDDAELARIGFTGSALDNATRLAKLAQASGLRGIVASAHEI
                     AAIREACGSDLRIVVPGIRPEGSDAGDQRRTKTPAAAIAAGADYIVVGRPVTDARDPR
                     AAAMAIVDSLV"
     gene            4170..5852
                     /gene="polX"
                     /locus_tag="VNN25_13680"
     CDS             4170..5852
                     /gene="polX"
                     /locus_tag="VNN25_13680"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXH92627.1"
                     /translation="MNKFDIARTLDEISDYLELSEANHFKALAFERAANSIRGLDREP
                     ADLIAGGTLLETPGIGKATAAIIEELVRTGTSKYAEELRGQYPPGIFELMRVPKLGLK
                     KIGVLYETLGIGSLGELEDAARAGRLASLKGFGAKTEQSILSGIEKARRRESHFLLPI
                     GVEVGEQIREHLAAIEAIEDAEVSGSVRRRLEIIRNVNIVIATRAQEAVVRALESFVE
                     RIERIDETTVKAFARNEIEVWFHFAKPDAFGATLLVTTGSQQFVEAFLARGGKLGRAG
                     TEKELFDKSGLAWVDPERREAGDDLERKRHKRLVEVSDLRGTFHVHTTFSDGRHTMRE
                     MLAAARERGFDYVGISDHSPAAFYARGLTVERLRDQQKEIAVQEKGVAPMRVFRGTEA
                     DILPDGSIDYDEKTLAKFDFVIASIHSRFTMTKDEMTERMLRALDNPYVTMLGHLTGR
                     KLLTRDGYTVEYERIFEKAAANNVMIEINGNPNRLDLDWRHIRRALDLGVTFTINPDA
                     HSTHEYNALISGTWVARKAGLTAKEIFNTKPVEEVAEYLEARRVKKNEPQRH"
     gene            complement(5912..7624)
                     /locus_tag="VNN25_13685"
     CDS             complement(5912..7624)
                     /locus_tag="VNN25_13685"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXH92628.1"
                     /translation="MRWSQYFINTLREVPADADVISQKLMMRAGMIRKVAAGIYTSLP
                     LGLRSMQKMEAIVREEMNRAGAIELSMPTIQPAELWMESKRWERYGKELLRVKDRHER
                     DFVYAPTAEEVITDTVRDAINSYRLLPVNLYQIQTKFRDEVRPRFGLMRGREFVMKDA
                     YSFHASRESLDETYEKMRVAYSAVFHRCALDHVAVDADTGNIGGSASHEFMVLAQSGE
                     DAVVSCPNCKYGANVEKATSKFFNADATPAALTELAPIHTPGTQSIDDVGKFLGKPTS
                     ELVKTLVFDTDKGCVMVLLRGDREGNEIKIKNHLGAEFLEMMPDTRFEAATGGPVGYC
                     GPVGTKAARVLADISLKGVANWVVGGNKKDTHIAGANPGRDFPEPEYGDFTTVFAGDP
                     CPRCETPLQIHRGIEVGHVFKLGTKYSEPMNCTYLDEKGARQPMIMGCYGLGIGRTVA
                     AAIEQSHDDEGIIWPMPIAPFEVVVTQIGREDNVRATAEKLYQKLRAVGVDVLLDDRD
                     ERPGVKFKDADLIGFPLRIGVGAKSLANGQIEWSFRRDRAKQLGAADEVLSRVIEEVT
                     KARV"
BASE COUNT         1512 a         2434 c         2379 g         1466 t
ORIGIN      
        1 cgcggtgttt cctggccagt acctctctcc ggatcaggtc gacggcatag gcgtcgcgct
       61 gcaaggcgta cttctgcacg tcctcacggg agcgtacggt cgcccaatcg atgggcgggt
      121 ccgcgagaag cacccggagc ctgtgcgcgc gcgggagcga cgcattgact tgacggacgg
      181 cgcgaaagaa gtcctcataa atcggcaagt cccagatgaa ttcgacctga gtcgtgtcct
      241 gccacacatg acgtaaggac gcataggcaa cgtcctcgcc gcgcgtgaag cgatcgatca
      301 catcctgata tcgcgcgtca ccgaattcga caacgatatc gtcgaccaca gaagagatcc
      361 ttggatcccg aagcagagaa acctggaagg cgtggccttg ctcgttaccg gcaaactcga
      421 tgttgccgag ggccacgatc acatgcgtcc tgaacgcgtc tgcaatcacc tcgaccgggt
      481 cgactggttt cgcgacggag ggcgtcacct gggcgggcgc catcgaagca gtttgggact
      541 gagccgaaag accaacagcg acagaaagca ctccaatcaa cgaaatcatt ttcttcacga
      601 ttggctcctc gattgcgtct tctcacgttg gctcgccgct tggcgggtca ttcattcagt
      661 cgttttgaga gagtacgatt cgccgcgcga atggttagga gttcctgccg ctatgcttgg
      721 cagatcgtcg aaatctttgc agacgttgat gtcggaaggg ctggcccgta ggctccgcga
      781 gcccagcgat gattggccgc ggcaaaaggg agctccgcgc gtccggcgag atcgatgtgg
      841 ccgaaggttg agactcggtg cgcgggagtc tctccaaaaa cgtcaacagc ccagacgggt
      901 gacgtagatg cgaggaactc tcgccccggt gaggttcatc ggatcgtcat gattacgtgc
      961 cgcccaacta gcagctcagc gtcactccgt cagttcgaca ttccagtacg cctcatccag
     1021 gaagttcttc cagatatccg ggaagttccg tccctgaagg cggtgaagcg gatgccaccg
     1081 cggccgcacc ggctgcctct tgatctgcaa gccggtctcg gtgagcagtt tgttgccctt
     1141 cttcacattg cagggaagac aggcgcagac gacgttctcc cacgtcgatt ttccgccgcg
     1201 cgagatcggc acgacgtgat cgagcgaaag ctccgacgac gagaacttct tgccgcagta
     1261 ctgacagcga ttgccgtccc gcagatagat gttgccgcgc gagaacttga cctcctgccg
     1321 cgggaggcgg tcgaagatca gcagttgcac gacgtgcggg atcaggatgt tgcggctggg
     1381 cgtgcggacg tagtgatcgt ctgcctggac ggggatatcg gcccagttgt cgaaatcgta
     1441 ggtccgatac tcggagtcga cggccttgac ctgccctttg tacaacaagg tcagcgcacg
     1501 gcgggcagag gtgatctgca ccgcctggaa gaccctgttg aggacgagca cgttaccgtc
     1561 gagcatagtt ctcactcgta acgcctcggg aaggccgagt tgtttccaat ccctgcccgt
     1621 gttccaaggc tgagtggaga catcatacac gggccgtgag cgagtccgga cctgtgcgcg
     1681 gcgcgaggat ttcagcatcg cgaaggacaa agttcagggc agataacgct gcccgcactg
     1741 cccgcaagtt gaaaaaagta cccgattgat agatagttat gagctaagac gaatccgaat
     1801 actgctctgc ggagcgcgtt ctgggtgtgg tttttgggag gcatgtatgc gcgttttgtc
     1861 acggatcgct ctcgttgtcc ttgtcgccgc tgccctcgtt ccggccgcgg cattcgccga
     1921 tcagtaccag gcgagctgcc ctctgacgct cgttgcgacc aacgcggcgc cgactgcgtt
     1981 cgccatctcg ccgcacggcg tcttccgctt cggctcgcag gtgtttgcgc tgcgcgggca
     2041 gacgctgacg acctacaccg tcaccgatct cggcgatatg cagatcgcgc gggaagactt
     2101 catcggagcg ctggccgcac gtgagaccaa aggcggcgtg gccttcgcca acggattcct
     2161 ctacgtgagc agcgaagcag gtctggagat ctacgacctg cgtagcgtcc gcgccggggg
     2221 cacagctccg atcctcatct cgcgcacgca aggacttcgc tatcaccgcc tcgccatcaa
     2281 tggcaacata ctcgccgcgc tctatcccgc gaccgacctg ccctgctccg cgaacggccc
     2341 aaccacgcca aactgtttca acgccatcga catctatgac gtcagcaatc ccctcgttcc
     2401 gacgcgcgtc agctcgcttt cgacgtctct gagcccgctc ggcggttaca acgacatcgc
     2461 gttcaacttc ggcttcctga tcgcggcggc gaacaccaac acggtggcgt ggaatctcac
     2521 gagcccgtcc gctccgacgc tcgtgggtag cctcgccgtc ccgggcacct tcctggtcag
     2581 caacggctcg aacctcctcg gcgtcggcaa caacaacgtg atcatcacct acaagatcgt
     2641 gccgagcagc gtgtccggca acttcttcgt ccccttcacc tatcactcca ccgcgaacct
     2701 tcaggtcgag cgcgccaacc cgatcatctt ccatccgcaa gcggtcttcg atgaagcgaa
     2761 cgaacggctg atcacgatga tcgatgagct cgattaccag acgctccagc cagcccgcac
     2821 gatcgcgttc gatgtcttcg attacgcaac ggcgatgttg gaaggacgcg atccgcggca
     2881 gtacgagcag gtctcctaca cgcagggcga tgaggtgaaa tggaacccgc tctccgtcgg
     2941 ctcgctggtc tatgtcgtcg gcgaaatgac cggcacgcag acctacggcg actgcccgtt
     3001 gatgagcggc cggatcgagt tcgacactat taacggcacg gtctgcggcg gctcgtacat
     3061 ccacggctgg gtgacgggta cgttgaagat caccaacgtc gagctcttcc tcgacggcgg
     3121 ctctctcggc tcggcatctc tggccggtgc accgcgcaac gacattccgt ccaccacgcc
     3181 cgtcttcacc tggcagatcg ccgcgaacct cgatgccacg acgaaaggcg tacacgtcct
     3241 tcgtgcggtc ggcaccgacg tgaacggcaa tcgccgccag ttctcgagcc agccgatcct
     3301 tttcccggga cccggatcca attgcgtagc gcgccggaag gcggcctcac actagcgaat
     3361 cgacgatggc cattgcggcc gcgcgcggat cgcgcgcgtc cgtgaccggc cttccgacga
     3421 cgatgtaatc cgcgcctgcc gcgatcgccg cggcgggcgt tttcgttctg cgctgatcgc
     3481 cggcgtcgct gccttcggga cgaatgccgg gcacgacgat gcgcaggtcg ctgccgcagg
     3541 cttcgcgaat cgcggcgatc tcgtgcgccg aggcgacgat gccgcgcaga ccggaggcct
     3601 gagccagctt tgccaggcgt gtcgcattgt cgagtgctga tccagtgaag ccgatgcgcg
     3661 ccaattccgc gtcgtcgagg ctcgtgagaa tcgtgacgcc gaggacgagc gtggtgtcga
     3721 cggcgcacgc gcggagcatc gcggttccgc ctgaagcatg caccgtgacc atctccgcgc
     3781 cgagggcagc tgccgcggcc accgcgtgct gcgcggtgtt cgggatgtcg tgaatcttga
     3841 ggtcgagaaa gaccttcgcg ccccgttcct gaacttcgcg cacgatcgat ggcccattcg
     3901 acacgaacgc ctgcagaccg atcttcagca gtcccaccga accgtggagc gcatcggcta
     3961 agcgaagaat ctcatcgcgc gaagagcggt cgatagcaac tatgagtcga ttgctcagcc
     4021 ggtcagttgc ttgcatggag ggattctacg cgggggtcgc ggttcggggg tcgggagtcg
     4081 gagagggacc atcgattttc cggtacgacc ggaactaata tcccactccg acacccgaca
     4141 cccgacaccc gacccccgac tccccgcaca tgaacaaatt cgacatcgcc cgcacactcg
     4201 acgaaatctc cgattacctg gagctgagcg aagcgaacca tttcaaggcg ctggccttcg
     4261 agcgcgccgc caacagcatt cgcggactcg atcgcgagcc cgcggacctc atcgcaggcg
     4321 gcacgctgct cgagacgccc ggaatcggca aagcgacggc ggccatcatc gaagagctcg
     4381 tacgaaccgg cacgtcgaaa tacgccgaag agcttcgcgg ccagtacccg cccggcatct
     4441 tcgagctgat gcgcgtgccg aagctcggat tgaaaaagat cggagtactt tacgagacgc
     4501 tcggcatcgg cagcctcggt gagctcgagg atgcagcgcg cgcaggccgc ctcgcatcgc
     4561 tgaaaggctt cggcgcgaag acggagcagt caatcctgag cgggatcgaa aaggcgcgcc
     4621 gccgcgaatc gcacttcctg ctgccgatcg gcgtcgaggt tggcgaacag atccgcgaac
     4681 accttgccgc gatcgaagca attgaagacg ccgaggtttc aggcagtgta cggcggcggc
     4741 tggagatcat tcgcaacgtc aatatcgtca tcgccacacg cgcgcaggag gcggtcgtac
     4801 gcgcgctcga gagtttcgtc gagcgcatcg aacggatcga cgaaaccacc gtgaaggcgt
     4861 ttgcgcgcaa cgagatcgag gtctggttcc acttcgccaa gccggacgca ttcggcgcca
     4921 cgctcctggt cacgacgggc agtcagcaat tcgtggaggc cttcctcgcg cgcggcggga
     4981 agcttggacg ggcaggaacg gagaaggagc tgttcgacaa gtccggtctt gcgtgggtcg
     5041 atccggaacg ccgcgaagcc ggcgacgatc tcgagcgcaa acgtcacaaa cgcctagtcg
     5101 aagtctcgga tcttcgagga acgtttcacg tccacacgac cttctccgat gggcgccaca
     5161 caatgaggga gatgctcgcc gcggcgcgcg aacgcggctt cgattacgta ggcatctccg
     5221 atcattcgcc cgccgcgttc tacgcccgcg gactgacggt cgagcgcctg cgcgatcagc
     5281 agaaggagat tgccgttcag gagaaaggcg tcgccccgat gcgcgtcttt cgcggcaccg
     5341 aggccgatat tcttcccgac ggatcaattg actacgacga gaaaacgttg gcgaaattcg
     5401 actttgtgat cgcatcgatc cattcgcgct tcaccatgac gaaggacgag atgaccgagc
     5461 gcatgcttcg cgctctcgac aatccgtacg taacgatgct cggccacctg accggccgca
     5521 agctcctcac acgcgacgga tacacagtcg aatacgagcg catcttcgag aaagcagccg
     5581 cgaacaacgt gatgatcgag atcaacggca atcccaaccg cctcgacctc gactggcgcc
     5641 acatccgccg cgccctcgac ctcggcgtca ccttcaccat caaccccgat gcccactcca
     5701 cccacgaata caacgcgctg atcagcggaa cgtgggtggc gcgcaaagca ggactgaccg
     5761 cgaaggaaat cttcaacacg aagccggtgg aggaagtggc ggagtatttg gaagcgcggc
     5821 gggtcaaaaa gaatgaacca cagagacact gaggacacag aggtgcgatg gttactcagt
     5881 gatctctgtg tctctgtggt gcaaagggtt cttacacccg cgcctttgtc acttcctcaa
     5941 tcactcgcga caaaacctca tccgcggcgc cgagttgctt ggcccgatcg cgccggaaac
     6001 tccactcgat ctggccgttt gcgaggctct tggcgccgac gccgatgcgt agcgggaagc
     6061 cgatcagatc ggcgtccttg aacttcacgc cgggacgttc atcgcgatcg tcgagaagca
     6121 catcgactcc cactgctcgc agtttctgat acagcttctc ggcggttgcg cggacgttgt
     6181 cttccctgcc gatctgcgtc acgacgactt cgaacggcgc gatcggcatc ggccagatga
     6241 tgccctcgtc gtcgtggctt tgctcgatcg cggcggcgac ggtacgtccg atgccgaggc
     6301 cgtagcagcc catgatcatc ggctggcgtg cgcccttttc gtcgagatac gtgcagttca
     6361 tcggttcgga gtatttcgtt ccgagcttga agacgtggcc gacctcgatg ccgcgatgaa
     6421 tttgcagcgg cgtttcgcag cgcgggcatg gatcgcccgc gaagactgtc gtgaagtcgc
     6481 cgtactccgg ctcggggaaa tcgcgccccg gattggcgcc ggcgatgtgc gtatctttct
     6541 tgttgccgcc gacgacccag ttcgcgacgc ctttcagcga gatgtccgcg aggacgcgcg
     6601 cggcctttgt cccgacggga ccgcagtagc cgacaggacc gccggttgcg gcttcgaaac
     6661 gcgtgtcggg catcatctcc aggaactcgg cgccgaggtg gtttttgatc ttgatctcgt
     6721 tgccttcgcg gtctccgcgc agaagcacca tcacgcagcc tttgtcggtg tcgaagacga
     6781 gcgtcttcac cagctccgag gtcggcttgc caaggaactt gccgacatcg tcgatggact
     6841 gggtgccggg agtgtggatc ggcgcaagct cggtcagcgc tgccggcgtt gcatccgcgt
     6901 tgaagaactt cgaagtcgct ttctcgacgt tcgctccgta cttgcagttc gggcacgaga
     6961 cgacggcgtc ttcgcccgat tgcgcgagca ccatgaactc gtgcgaagcc gagccgccga
     7021 tgttgccagt gtcggcatcg acggcgacat gatcgagcgc gcagcgatgg aagacggcgc
     7081 tgtaggcgac gcgcatcttc tcgtacgtct cgtcgagcga ctcgcggctg gcgtggaagg
     7141 agtaggcgtc cttcatcacg aactcgcgcc cgcgcatgag gccgaagcgc ggacgcactt
     7201 catcgcggaa cttagtctgg atctgataga gattcaccgg cagcagacga tacgaattga
     7261 tggcgtcgcg gaccgtgtcg gtgatgactt cttcggccgt cggcgcgtag acgaaatctc
     7321 tctcgtgccg gtctttgacc cgcagcagct ctttgccgta gcgctcccag cgtttcgact
     7381 ccatccacag ctcggcgggc tggatcgtcg gcatcgacag ctcgatcgcg ccggcgcggt
     7441 tcatctcttc gcggacgatc gcctccatct tctgcatcga gcggaggccg agcggcagag
     7501 aggtgtaaat gccggcggca acttttcgga tcatgccggc gcgcatcatc agcttctgac
     7561 tgatgacgtc ggcgtcggcg ggaacttcac gcaacgtgtt gatgaaatat tggctccagc
     7621 gcatggggcc gggattgtat ctcgcggttg cggcgtcgtg acgtagggtg tgggggagtg
     7681 gggtgtaggg agtggggagt ggaagccaag aatcacgcag tcaaaggcat tctgtcatcg
     7741 cgattcgaca ggcgataagc gagatcgcga gattcttctc ccacacccca c
//
LOCUS       DATLVW010000449         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_101945, whole genome shotgun sequence.
ACCESSION   DATLVW010000449 DATLVW010000000
VERSION     DATLVW010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101945"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2118
                     /locus_tag="VNN25_13690"
     CDS             <1..2118
                     /locus_tag="VNN25_13690"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXH92629.1"
                     /translation="LEVFIGSIMVVYFGFLLKLSFVEVLVIVLFSLVGIAGSAGVAWF
                     GIRVNTFANSRTAFASLRGKPFPTYAIPLKAGMSIGMLLISTELVLMLFILLFVPRDY
                     AGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCT
                     GDNAGDSVGPTADGFETYGVTGVALITFILLAVSDPIVQTQLLVWIFVMRVLMVITSA
                     VSYWVNGAVAAAKYRNADSMNFEAPLTSLVWITSILSVGVTYAVSYLLIPTLGGDASM
                     WWKLSTIITCGTLAGAIIPELVKVFTSTESRHVREVVTASREGGASLNILAGLIAGNF
                     SAYWMGITIAALMGVGAWISSMFPIGAGALMIAPAIFAFGLIAFGFLGMGPVTIAVDS
                     YGPVTDNAQSVFELSTIETMPGIEAEIKRDFGFDPSFAKGKDFLEANDGAGNTFKATA
                     KPVLIGTAVVGATTMIFSIIVMLTNKLTTDMDKLSLMHPQFLLGLVVGGAVIYWFTGA
                     STQAVSTGAYRAVEFIKQNMHLDAGATKASVEDSKKVVAICTQYAQKGMFNIFLTVFF
                     STLAFACFEPFFFIGYLISIALFGLYQAIFMANAGGAWDNAKKIVEVELKEKGTPLHS
                     ACVIGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAVEMTKNGQQGLTRILSVIFF
                     IISMIFVYRSFYGMRIQSQSAAH"
BASE COUNT          381 a          753 c          608 g          471 t
ORIGIN      
        1 ctcgaggtct tcatcggctc gatcatggtg gtctacttcg gcttcctctt gaagctgagc
       61 ttcgtcgaag tcctcgtcat cgtgctcttc agcctggtcg gcatcgccgg ctcggcgggc
      121 gtggcgtggt tcggcatccg cgtcaacacc ttcgccaact cgcgcaccgc cttcgcctcg
      181 ctgcgcggca agccgttccc gacctacgcc atcccgctca aggccggcat gtcgatcggc
      241 atgctgctca tctccactga gctcgtgctg atgctcttca tcctgctctt cgtgccgcgc
      301 gactacgccg gtccttgttt catcggcttc gccatcggtg agtcgctcgg cgcggccgcg
      361 ctccgcatcg ccggcggcat cttcaccaag atcgccgaca tcggctccga cctcatgaag
      421 atcgtcttca acatcaagga agacgatgcc cgcaacccgg gcgtcatcgc cgactgcacc
      481 ggcgacaacg ccggcgactc ggtcggcccc actgccgacg gcttcgagac ctacggcgtc
      541 accggtgtcg cgctcatcac cttcattctt ctggcagtct ccgatccgat cgtgcagacg
      601 cagctcctcg tctggatctt cgtcatgcgc gtcctcatgg tcatcacatc ggccgtctcg
      661 tactgggtca acggcgccgt cgctgccgcc aagtaccgca acgccgactc gatgaacttc
      721 gaagcaccgc tgacgtcgct cgtctggatc acatcgatcc tgtctgtcgg tgtcacatac
      781 gccgtctctt atctcctcat ccccaccctc ggcggcgatg cctcgatgtg gtggaagctt
      841 tcgacgatca tcacctgcgg cacgctcgca ggcgcgatca tcccggagct cgtaaaagtc
      901 ttcacctcca cggaatcgcg tcacgtgcgg gaagtcgtca ccgcctcgcg cgaaggtggt
      961 gcgtcgctga acattctggc aggtctcatc gccggtaact tcagcgccta ctggatgggc
     1021 atcaccatcg ccgcactcat gggagtcggc gcctggatca gcagcatgtt cccgatcggc
     1081 gccggcgcgc tcatgattgc gcccgcgatc ttcgcgttcg gtctcatcgc cttcggcttc
     1141 ctgggcatgg gtccggtgac gatcgccgtc gattcctacg gtcccgtcac cgacaacgcg
     1201 cagtccgtct tcgagctctc gacgatcgag acaatgcccg gcatcgaagc cgagatcaag
     1261 cgcgacttcg gtttcgatcc gtcattcgcg aaggggaaag acttcctcga agcaaacgac
     1321 ggcgccggca acacgttcaa agcgacggcg aagcctgtgc tcatcggcac ggcggtcgtc
     1381 ggcgcaacaa cgatgatctt ctccatcatc gtgatgctca ccaacaagct cacgaccgac
     1441 atggacaagc tctcgctcat gcacccgcag ttcctcctcg gactcgtcgt cggcggcgcg
     1501 gtgatctact ggttcaccgg cgcatcgacg caggccgtct ccaccggagc gtaccgcgcg
     1561 gtcgaattca tcaaacagaa catgcacctc gatgccggcg ccacgaaggc gtccgtcgag
     1621 gattcgaaga aggtcgtcgc catctgcacg cagtacgcgc agaagggcat gttcaacatc
     1681 ttcctgaccg tgttcttctc aactcttgcc tttgcttgtt tcgagccgtt cttcttcatc
     1741 ggctacctga tctcgatcgc tctcttcggt ctctaccagg ccatcttcat ggccaacgcc
     1801 ggcggcgcgt gggacaacgc caagaagatc gtcgaggtcg agctgaagga gaagggcacg
     1861 ccgcttcatt ctgcctgcgt catcggcgac acggtcggcg acccgttcaa ggacacctcg
     1921 tcggtggcca tgaatccggt catcaagttc acgacgctct tcggtcttct ggccgtcgag
     1981 ctggccgtgg aaatgacgaa gaatggtcag cagggactga cgcgaatcct gtcggtaatc
     2041 ttcttcatca tctcgatgat cttcgtctac cgctcgttct acggaatgcg catccagtca
     2101 cagagcgcgg cgcactgaac agggtttctc ggagtttcgt cgccccctca tccggccttc
     2161 ggccaccttc tcccccacaa aaagcgtggg ggagaagggg ctcgattgga gcg
//
LOCUS       DATLVW010000450         3996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_309625, whole genome shotgun sequence.
ACCESSION   DATLVW010000450 DATLVW010000000
VERSION     DATLVW010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309625"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1923)
                     /locus_tag="VNN25_13695"
     CDS             complement(49..1923)
                     /locus_tag="VNN25_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXH92630.1"
                     /translation="MKRFVLVLATLFAAIALHAAPISLRVDATGVHSNVYHAHLTIPV
                     TAGPLTLYYPKWIPGEHGPTGPINGLTGLQFMANGKSVTWQRDLVDMFALNLEVPAGT
                     TLLDADLDYIAPAPGANFTAGASTSPRLAVLSWNTVLLYPKGTASDALSYSASLRIPS
                     GWQFATPLETRGHSGDDIEFETASLTTLVDSPVQLGTTLRKIDIPNKTGLRHTLNLLS
                     ESADAIQTPDDFAAQYGRLVDETSTLFGANHYRHYDWLFTLSDAVAHFGLEHHESSDD
                     RVAENRLREDPSRKWVSTLLAHEFVHSWNGKYRRPAGLATGNYDKPMTGELLWVYEGL
                     TEYLGDLLPVRAGILSAEDYRENLAETYAQMAQHTGRTWRPLLDTAVAAQLLYEAPGA
                     SMNWRRSVDYYPEGELLWLDADMTIRSLTSGKRSLDDFCRAFYGGTSGHAELKPYTFD
                     DLVAALNSVAPHDWKTFLNERLISTSSMPPVGGIEQAGWRLVYNDKPNTALQFYEGER
                     KGADLSASLGFSVQEGGSVGDVVPGSIAARSGLAPGAQIVAVDGRMFTIARLRAIVKA
                     SASSKGPIDLLVRNGDFFVTESIDYHGGERYPHLERVTGKPDRMEALTKPLVPVAAKK
                     "
     gene            complement(2028..2780)
                     /locus_tag="VNN25_13700"
     CDS             complement(2028..2780)
                     /locus_tag="VNN25_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH92631.1"
                     /translation="MISLQHVDKTLGGKKVLDDMSIDVGRGDSFVIVGGSGVGKSVTI
                     KHIIGLIKPDRGHVVVDGQDITEMRPVDLNRFRRKFGMSFQEGALFDSMSVFENIAFP
                     LRRHTKFTETQIRERVDECLDLVHLEGVSSSRPSELSGGMRRRVGFARAISLKPEILL
                     FDEPTTGLDPVISDVIADLIVEMDRTLNTTTVTITHDMKVAFKIADRVAMLYKGRIVE
                     EGTPETFQQSQNPIVQQFIQGRAEGPLTAMDN"
     gene            complement(2819..3586)
                     /locus_tag="VNN25_13705"
     CDS             complement(2819..3586)
                     /locus_tag="VNN25_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92632.1"
                     /translation="MNILERIGRVFIRLFEETGLWFRMLARTFVWTFRPPFEGGEWLR
                     QMVRVGVDSIPVVFLTTMFTGMVMALQTYNGFHRVHAENFVGSVVALAMLRELSPVLV
                     ALMVTGRVGSSMAAEIGTMRVTEQLDALEALATDPVQYLFVPRVIAGIVMVPLLTVLG
                     DALGVGGGYLVGVKMMGANPVVYEQNTYQFLHMNDVWSGLIKSAFFGLILTLTGCVRG
                     YYTRGGAEGVGRATTAAVVSASLIILLTDFFLTKLLF"
     gene            complement(3583..>3996)
                     /locus_tag="VNN25_13710"
     CDS             complement(3583..>3996)
                     /locus_tag="VNN25_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HXH92633.1"
                     /translation="TEIMRLKELPAGHAIGYGTTFRTSRPSRIATLPVGYADGYDRLL
                     SNNADVLVRGKRAPVVGRVSMDLVTIDVTDIAGVTVGDEVILIGEQGGEIITANELGL
                     KSQTIGYEVLCRISARVPRLYPDQGAFRIRSRFAE"
BASE COUNT          817 a         1199 c         1175 g          805 t
ORIGIN      
        1 tgtcggcgga tcagtagatg ttcagtgtcc attgaacgat tgcccggctt atttcttcgc
       61 cgccaccgga accaacggct tggtcagggc ttccatccga tcaggcttgc cggtcactcg
      121 ctccagatgc ggatagcgct ctccgccgtg gtagtcgatc gattccgtca caaagaaatc
      181 accattcctg acgagaaggt cgattggacc tttagagctc gccgaggctt tcacaatggc
      241 tcgcaatcgc gcgatggtga acatgcggcc gtcgacggcg acgatctgcg ctcctggagc
      301 gagaccgctt cgagcggcaa tcgatcccgg tacgacatcg ccgacggatc caccctcctg
      361 aacactgaat ccgagtgatg cgctgaggtc ggcacctttg cgctctccct catagaactg
      421 aagcgccgtg tttggcttat cgttgtagac cagccgccat ccggcttgct cgatgccgcc
      481 aaccggaggc attgaagagg tcgagatcag gcgctcgttc agaaatgtct tccaatcgtg
      541 cggcgccaca ctgttgagcg cggcaacgag atcgtcgaac gtatacggct tcagttcggc
      601 gtgcccgctc gttcctccgt agaatgctcg gcagaaatcg tcgagggagc gtttgccgga
      661 cgtcagcgac cgaatcgtca tatccgcatc cagccagagc agctccccct cggggtaata
      721 atcgacgctg cgccgccaat tcatcgatgc gccgggcgcc tcgtacagca actgtgcggc
      781 aacggccgta tcgagtagtg gtctccacgt gcgtccagtg tgttgcgcca tctgcgcata
      841 ggtttcggcg aggttctcgc ggtaatcttc ggccgagagg atgccggccc ggaccggaag
      901 gagatcgccg agatattcgg tgagtccttc gtatacccac aggagctccc cggtcatcgg
      961 cttgtcataa ttgccggtgg ccaagcccgc tggacggcga tatttgccgt tccaggagtg
     1021 cacaaactcg tgcgcgagta gcgtggagac ccatttccgg ctcggatctt cgcgcagacg
     1081 attctcggcc acgcgatcat cgctcgattc gtggtgctcg agaccaaagt gcgcgacagc
     1141 atcgctcaac gtgaagagcc aatcgtagtg tcgatagtgg ttcgcgccga acagcgtcga
     1201 cgtctcatcg acaagtctgc cgtactgagc cgcaaaatcg tcaggcgtct ggatcgcgtc
     1261 cgcactctca gacagcagat tcagcgtatg gcgcagcccg gttttattgg ggatgtcgat
     1321 cttgcgcaga gtcgttccga gctgcaccgg agaatcgacc agtgtcgtga gtgatgcggt
     1381 ttcgaactcg atgtcgtcgc cggagtggcc ccgcgtttcc agcggagtcg cgaattgcca
     1441 gcccgatggg atccgcaacg aagccgaata ggaaagggca tcgctggccg tacccttcgg
     1501 atagagcagg accgtgttcc agctgaggac cgccagccgc ggcgaggtcg atgctcccgc
     1561 agtgaaattc gcgcccggag cgggggcgat gtaatcgagg tccgcgtcca gcaacgtcgt
     1621 gccggcggga acttcgagat tcaacgcgaa catgtcgacc aggtcacgtt gccatgtaac
     1681 gctcttgccg ttcgccatga attgcaaccc cgtcaggccg ttgatgggac ccgtaggtcc
     1741 atgctccccg ggaatccatt tcgggtaata gagtgtgagc ggcccggccg tcaccggaat
     1801 agtgagatgc gcgtggtaga cgttcgagtg aacgccagtt gcatctacgc gcagagagat
     1861 cggcgccgca tgaagcgcga tcgcggcgaa cagtgtggcc agaaccaaga caaatcgctt
     1921 cataacgctc ctttgtgaat ggacaattga taattgacaa ttgacaatgt gcactgcagg
     1981 ccagcgtacg ttccgtatga cggcctctcg ttgtcaattg tcaattatca attatccatt
     2041 gcggtcaacg gtccttccgc tcttccctgg atgaactgct ggacgattgg attctgcgac
     2101 tgctggaacg tctcgggcgt gccttcttcc acgatccgtc ctttgtagag catggctacg
     2161 cggtccgcga ttttgaatgc cactttcatg tcgtgcgtga tcgtcacggt ggtcgtgttc
     2221 agcgtgcggt ccatttccac gatcagatcg gcaatgacgt cgctgatgac cggatcgagt
     2281 ccggtcgtcg gctcgtcaaa cagcagaatc tccggtttga gcgagatggc acgcgcgaaa
     2341 ccgacgcgtc ttcgcatgcc tcccgagagc tcggatggac gactcgaaga cacgccttcg
     2401 agatggacga gatcgaggca ttcgtcgaca cgttcgcgaa tctgcgtctc ggtgaacttc
     2461 gtatggcgcc gcagcgggaa ggcgatgttc tcgaagactg acatcgagtc gaagagcgcc
     2521 ccttcctgaa acgacatccc gaacttccgg cggaatcgat tcaggtccac gggccgcatc
     2581 tcggtgatgt cctgcccatc gacgacaaca tgtccgcgat ccggcttgat caggccgatg
     2641 atgtgcttga tcgttacgct cttccccaca ccggaaccgc cgacgatcac gaacgagtcg
     2701 ccacgcccga cgtcgatcga catgtcgtcg agtaccttct tcccgccgag cgtcttgtcg
     2761 acgtgttgga gagagatcat gggcgtgttg tcgttcatat tgtcgggttg ctgacaagtc
     2821 aaaacaacag cttcgtcaaa aagaaatcgg tcaacagaat gatcagcgac gcgcttacga
     2881 ccgctgccgt cgtggcgcgg cccactcctt ccgcgccgcc tctcgtgtaa tagccgcgca
     2941 cacagccggt cagcgtgagg atcaacccga agaacgccga cttgatcaat ccgctccaaa
     3001 catcgttcat atgtagaaat tgatacgtgt tctgctcata aaccacggga ttcgcgccca
     3061 tcattttcac gccgacgagg tagcccccgc cgacgccaag tgcgtcgccg aggacggtga
     3121 ggagcggaac catgacaatg ccggcgatga cgcgagggac gaagaggtat tgcacgggat
     3181 ccgttgccag cgcttcgagt gcatcgagtt gctcggtgac gcgcatcgtc ccgatctcag
     3241 ctgccatcga cgagccgacg cggcccgtaa ccatcagcgc caccagcacc ggcgatagct
     3301 cgcgcaacat tgccagcgct acgacactgc caacgaaatt ctcggcatgg acgcgatgaa
     3361 aaccgttgta cgtctgcagc gccatgacca tgccggtgaa catcgtcgtc aggaaaacga
     3421 cagggatgga gtcgacgccg acgcgcacca tctggcgcag ccactcgccg ccctcgaatg
     3481 gcggccggaa cgtccagaca aatgtcctgg ccagcatccg gaaccagagg ccggtctctt
     3541 cgaagagacg gatgaaaacg cggccgatcc gttccaggat gttcattcgg cgaaacggga
     3601 gcgtatccga aatgcgccct gatccggata aagccgggga acgcgggcgc tgatgcggca
     3661 gagcacttcg tagccaatcg tctgcgactt cagccccagc tcattggcag tgatgatttc
     3721 gccgccttgc tcgccgatga gaatgacttc gtcacctaca gtcacgccgg cgatatccgt
     3781 gacgtcgatc gtgacaaggt ccatcgacac gcgtccgacg actggagcgc gtttcccgcg
     3841 cacgagaacg tcggcattgt tcgacagcag gcggtcgtag ccatcggcgt atccgaccgg
     3901 caacgttgca atgcggctcg gccgcgaggt gcggaacgtc gtgccgtagc cgatcgcgtg
     3961 ccctgccggc agctccttca gccgcatgat ctcggt
//
LOCUS       DATLVW010000451         4323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_309641, whole genome shotgun sequence.
ACCESSION   DATLVW010000451 DATLVW010000000
VERSION     DATLVW010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309641"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VNN25_13715"
     CDS             <1..905
                     /locus_tag="VNN25_13715"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HXH92634.1"
                     /translation="LGMMAAEGALQTAGRTREEIDALIFATMTPDHFFPGNGPVVQAK
                     MGFRESLPAFDIRQQCSGFLYGLDLADSLILSGKYKRILLIGGDVHTPFMPWQNGWAT
                     TIGLEDREVTAEEREANTKIRDRVVLFGDGAGAVVVEAVDDDSRGFLASKLYTNGSNI
                     EALYTRGVGFRLRPFVTHQQIDDSDIIPDMDGREVFRQALSKMPESVREVCAAAGKSV
                     SDIDILLVHQANLRIVEGVAKQLGLSPDRVPHNIERYANTTAGTLPILYHECVTEGRI
                     KPGMLVCFTALGSGLHWGAALYRA"
     gene            complement(911..1411)
                     /locus_tag="VNN25_13720"
     CDS             complement(911..1411)
                     /locus_tag="VNN25_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92635.1"
                     /translation="MADIDFGPNAKASDVSPFSLGVLRDVMTAANVANVIISSTQRSP
                     ADQARVMYNNLETQGVAAQRRLYKPPGQAVIDVYVAGKAANQTPDAIKAAMTAKINEL
                     GPETVSHHAADPKILNVFDVAPSSVSDVDAFQNAAKADSRVSKFLTPPNDPGLHFEIP
                     QPQNPD"
     gene            complement(1571..1975)
                     /locus_tag="VNN25_13725"
     CDS             complement(1571..1975)
                     /locus_tag="VNN25_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH92636.1"
                     /translation="MSENDNIQKMQSLYAAFGRGDINMILANVSDGADWGTDTVATAV
                     PWYGIRRGREGVADFFATLEREVEFTTFEPRVFAATGDQVFVHVDIAYKLKKNGRSAS
                     TGSVHEFTLRDTVVIRFRSFEDTAAVRDSWNG"
     gene            2145..3395
                     /locus_tag="VNN25_13730"
     CDS             2145..3395
                     /locus_tag="VNN25_13730"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HXH92637.1"
                     /translation="MFVLYEVLLYLAFVLALPYFLLTGALRGKYLANFPARMGFYRTP
                     AAAHDLWIHAVSVGETMAARPVVEEVLRRRPTTTIVVTTTTITGQAQAHKLFPNATVT
                     YFPFDFTVSVKRFLSHHQPRVFATMETEIWPNVTRLSRKRGVRMLLANGRISDRSFPR
                     YRLLKGLVGPILRKYDHILAREETDRERFIAIGAPAGIVETSGNVKFDYVPDERPLEI
                     GPRLEALIAGRKVLILGSTMEGEDEELVPLLERFIAAHNAFVIIGPRKPERFDVVAAL
                     LTASKIRFVRRSEWADGAADVLLLDTFGELAKIYRYATASFIGGSLLLPTGGHNPIEP
                     AAAGVPVTFGPHMSNFREIAEIFLREGGAVESRTAGDVLAFAGRIFDDDAARAEAGEK
                     ARRTVEKNRGAAERTASRIIEMLA"
     gene            3395..4279
                     /gene="lpxK"
                     /locus_tag="VNN25_13735"
     CDS             3395..4279
                     /gene="lpxK"
                     /locus_tag="VNN25_13735"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXH92638.1"
                     /translation="MILLKPIELLWRSVNRVRRGLYRRGILKGRRLPRPVVSVGNLAA
                     GGAGKTPAVIAIARALTERGLIVTILTRGYGRAATEGGLVTSLDPGQFGDEPVLIKSK
                     LQKVHVIVGSKRYDNAIRFLTDNDCDLFILDDGFQHLKLARDLDVVIDAGRGVMREGR
                     SALADADLVIQRNLRIVVPRELAARRVFAFAGVADNEQFFASLREHGLNVVGTRGFRD
                     HHRYTAADIAAIKSAALDAGAEATVTTEKDKVKIDDRGIIAIAAEMIIEPGVIEAIAR
                     VVSRKAESRDKGSEIRDQ"
BASE COUNT          806 a         1329 c         1372 g          816 t
ORIGIN      
        1 acctcggcat gatggccgcc gagggggcgt tgcagacggc aggacggacg cgcgaggaga
       61 tcgacgccct catcttcgcc acgatgacac ccgaccactt cttccctggc aacggtcccg
      121 tcgtccaggc gaagatgggc ttccgcgaat cactccctgc cttcgacatc cgccagcagt
      181 gctccggctt tctctacggc ctcgatctgg ccgactcgtt gatcctgagc ggcaagtaca
      241 agcgcattct cctgatcgga ggcgacgtcc acacaccgtt catgccgtgg cagaacggct
      301 gggcgacgac catcggtctc gaggatcgcg aagtcacggc tgaggagcgc gaggccaaca
      361 cgaagattcg cgaccgggtc gttctgttcg gcgatggtgc cggtgcggtc gtggtggaag
      421 ctgtcgacga tgacagccgc ggcttcctgg cgtcgaagct gtacacgaac ggctcgaaca
      481 tcgaggcgct gtacacccgc ggcgtcggct tcaggcttcg cccattcgtt acgcaccagc
      541 agatcgacga cagcgacatc atcccggaca tggacggccg cgaggtcttc aggcaggcgc
      601 tgtcgaagat gcccgagtcc gtgcgcgaag tctgcgcggc ggcgggaaag agcgtgtcgg
      661 acatcgacat tctgctcgtt catcaggcca accttcgtat cgtcgaaggc gtggcgaaac
      721 agctcggact cagccccgat cgcgttccgc acaacatcga gcgctacgcg aacacaaccg
      781 ccggcacgct gccgatcctg tatcacgagt gcgtgaccga aggccggatc aagcccggca
      841 tgctggtctg cttcaccgcc ctcggatcag gcttgcactg gggcgcggcg ctctaccgcg
      901 cctgaacccg ttaatcgggg ttctgcggct gcgggatctc gaagtgcagg ccaggatcgt
      961 tcgggggagt caggaacttc gatacgcggc tgtctgcctt ggccgcgttc tggaacgcgt
     1021 caacgtccga cactgacgac ggcgcgacgt cgaatacgtt caggattttc ggatcggccg
     1081 cgtgatgcga caccgtttcc gggccgagct cgttgatctt tgcggtcatc gcggccttga
     1141 tcgcgtcggg cgtctgattc gcggccttgc cggcgacata gacgtcgatg actgcctgtc
     1201 cgggtggttt gtacagccgc cgctgggcag cgacgccctg ggtctcgaga ttgttgtaca
     1261 tcacgcgagc ctgatcggcg ggactcctct gcgtgctgga gatgatgaca ttggcgacgt
     1321 tcgccgcagt catgacatcg cgcaatacgc caagggagaa cggcgataca tcagacgcct
     1381 tcgcgttcgg gccaaagtcg atatccgcca ttcagtaagg atactacgca cgggggagtc
     1441 gcttgtcggg ggtcgggggt cggagcaaag gccgcgaagt attcagatcg gattgggctc
     1501 caaccaaacg cccacgatgg cgttgatttc tccgacaccc gacacccgac ccgcgacccc
     1561 gcgtaaggtc tcaaccgttc cagctgtcgc gaacggcggc ggtgtcttcg aaggatcgga
     1621 agcggatcac gacggtatcc cgcaacgtga attcgtggac cgagcctgtc gacgccgagc
     1681 ggccgttctt cttcagtttg taggcgatgt cgacgtggac gaaaacctgg tcgcctgttg
     1741 ccgcaaagac tctcggctcg aacgtcgtga attcgacttc gcgctcgagc gtggcgaaga
     1801 aatcagccac cccttcccgt ccgcgccgga tgccgtacca gggaacagcc gtggcaacgg
     1861 tatcggtgcc ccagtcggcg ccgtccgaaa cgttggccag gatcatgttg atatccccgc
     1921 gtccgaatgc ggcatagaga ctctgcattt tctggatgtt gtcgttctcg ctcatcgatc
     1981 tgccctctgc tcgtcatcta cggaccggaa cgggcggtgg atgggtggcg ggttgcctgt
     2041 tgcgtgagct gtgacgcgga ctccgactcc cgactcccga acagcgaccc ccccaattca
     2101 ctcccacccc ccacacccaa ccccccacac cctatgattc acaaatgttc gtcctctacg
     2161 aggtgctgct ctacctcgcg ttcgttcttg cgctgcccta cttcctgctg acgggggctc
     2221 tgcgcgggaa gtaccttgcc aattttccgg ccaggatggg cttctatcgg actccggcgg
     2281 cggcacacga tctatggatc cacgcggtgt cggtcgggga gacgatggcg gcgcggcctg
     2341 tcgtcgagga ggtgctgcgg cgacggccga cgacgacgat cgtcgtcacg acgacgacga
     2401 ttacaggtca ggcgcaggcg cataaactct tccccaacgc caccgtgacg tactttccgt
     2461 tcgacttcac ggtaagcgtg aagcgcttcc tgagccatca tcagccacgc gtcttcgcca
     2521 cgatggagac cgagatctgg ccgaatgtga cgcggctttc tcgaaagcgc ggagtgagga
     2581 tgctgctggc caacgggcga atctctgacc gctcgtttcc gcggtaccgg ctactgaagg
     2641 ggctcgtcgg gcccattctc cgaaagtacg atcacatcct tgcccgtgag gaaacggatc
     2701 gcgagcgatt catcgccatc ggcgctcccg ccggcatcgt cgagacgagc ggcaacgtga
     2761 agttcgatta cgtccccgac gagcgtccgc tcgagatcgg gccgcggctc gaagcgctca
     2821 tcgcaggccg caaggtgctc atcctcggct cgacgatgga gggcgaggat gaggagctcg
     2881 tgccgctgtt ggagcgtttc atcgccgcac ataacgcgtt cgtgatcatc gggccgcgaa
     2941 agccggagcg attcgatgtc gtggccgcgc tgctcactgc atcgaagatc cgctttgtcc
     3001 ggcgcagcga atgggctgat ggcgcggcgg atgttctgct cctggacacc ttcggcgagc
     3061 tggcgaagat ctatcgctat gccaccgcgt cgttcatcgg cggaagcctg ctccttccga
     3121 ccggcgggca caatccgatc gagcccgcag cagcgggagt gccggtcaca ttcggcccgc
     3181 acatgtcgaa cttccgcgag atcgccgaga tctttcttcg ggagggaggc gcggtggagt
     3241 cgcgaaccgc cggcgatgtt ctcgcgttcg cgggacggat attcgatgac gacgccgctc
     3301 gcgccgaggc gggagagaaa gcgcggcgca ccgtcgagaa gaaccgaggc gcggcggagc
     3361 ggaccgcctc ccggatcatc gagatgctgg cgtgatgatc ctgctcaaac cgatcgagct
     3421 gctctggcgc agtgtgaacc gtgtgcggcg tggtctctac cggcgcggca ttctgaaggg
     3481 ccgccgtctg ccgcggcccg tggtttccgt cggcaacctg gctgcgggag gggcgggaaa
     3541 gactccggcc gtcatcgcca tcgcgcgcgc actcacggag cgtgggctga tcgtgacaat
     3601 cctgacgcga ggctatggac gagctgccac cgaaggggga ctcgtgacat cgctcgaccc
     3661 cggacaattc ggtgacgagc cggtgctgat taaaagcaag ctgcaaaagg tgcacgtaat
     3721 cgttggatca aagcgatatg acaatgcaat acgatttttg actgataatg attgcgattt
     3781 attcatcctc gacgacggct tccagcacct gaaactcgcg cgcgatctcg acgtcgtgat
     3841 cgatgccggg cgcggtgtga tgcgcgaagg gcgctccgcg cttgccgatg ccgatctcgt
     3901 gattcaacgc aacctccgga tcgtcgtacc gcgggaattg gctgccaggc gcgttttcgc
     3961 gttcgccggc gtggccgaca atgagcagtt cttcgcttcg ctacgcgaac atgggctgaa
     4021 tgtcgtcggc acgcgcggct ttcgtgatca tcaccgctac acggcggcgg acatcgccgc
     4081 gatcaagagc gcagcgctcg atgcgggagc cgaggccacc gtgactacgg aaaaagacaa
     4141 ggtcaaaatc gacgaccgtg gcatcatcgc catcgccgct gaaatgatca tcgagcccgg
     4201 ggtgattgag gcgattgcga gagtcgtgag cagaaaagca gaaagcagag ataagggatc
     4261 agagatcaga gatcagtaga gtcaagatct atgttcccgg tttctgatca gagctgaccg
     4321 gcg
//
LOCUS       DATLVW010000452         5704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_101969, whole genome shotgun sequence.
ACCESSION   DATLVW010000452 DATLVW010000000
VERSION     DATLVW010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101969"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VNN25_13740"
     CDS             <1..187
                     /locus_tag="VNN25_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HXH92639.1"
                     /translation="LLTPEAIEGVIDLIPESWLGDEPRFATIAEHRLGYRDYLMQRLA
                     SPRKWMEDAVHARTLRV"
     gene            165..554
                     /locus_tag="VNN25_13745"
     CDS             165..554
                     /locus_tag="VNN25_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HXH92640.1"
                     /translation="MHERFVFDYAVVRIVPRVDRGEFLNAGVIVFSPTAGFLDCRIEL
                     DHARLLALAPSIDIAVVESYLDAMPKICAGGGEAGSIGDLPQRARFHWLVAPRSTVIQ
                     TSAVHSGVHEDPRAALEGLFEKLVRTV"
     gene            complement(568..1590)
                     /locus_tag="VNN25_13750"
     CDS             complement(568..1590)
                     /locus_tag="VNN25_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HXH92641.1"
                     /translation="MTPQRGQFLVYQTQDGKLKIDVRFEGETVWLTQQQMAELFQTTK
                     QNVSLHILNVFSECELAEDSVVKDSLTTAADGKNYSTRFYNLDVIISVGYRVKSHRGT
                     QFRMWATQRLREYIVKGFVLDDERLKNPDQPFDYFEELTRRIQDIRTSERRFYQKITD
                     IYATSIDYDPTQDISIRFFKTVQNKVHWAITGQTAAEIVHERVDAAKPNLGLTNWRGA
                     TIRKDDVAIAKNYLTEPELAALNNLVEQYLVFAEGQAMRRVPMHMGDWIKKLDGFLSL
                     NERDILTHAGHISHEMALTKAELEYDKFKALASSASLPVDADFERATKLLPKLSRTKK
                     PKREKA"
     gene            1759..2223
                     /gene="tsaA"
                     /locus_tag="VNN25_13755"
     CDS             1759..2223
                     /gene="tsaA"
                     /locus_tag="VNN25_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HXH92642.1"
                     /translation="MTDILFTPHPIGFARTPFKDTTQVPKGPGTKHEADGTLEILPEF
                     AQGLKDIEGFSHLFVIWVFDRSDGYDLLGAPPGETVQHGVFATRSPRRPNPIALTVVE
                     LLRREGTTLHVHGVDMLDRTPILDIKPYMSSIPIDKLRRGWLEDTERRAGKS"
     gene            2269..3486
                     /locus_tag="VNN25_13760"
     CDS             2269..3486
                     /locus_tag="VNN25_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92643.1"
                     /translation="MNYLALLTSSLRALLRNKMRSLLTVLGITIGIAAVICVVAIGRA
                     GQVRVEQQLNNLGDNFVWIEAGGRAVNGVRTGPRGTKTLTIGDAFAIRNQIPLIKSVS
                     PNVDASTQVIYGNQNWFTSYRGVSPEYFDIKRWVIDEGAAFTADDVERAADVCVIGAT
                     IRSQLFGVANPIGQVIRLKSLPCKVVATLKPKGLSISGQDQDDTILLPYTTAQKKLAG
                     VTWLDDILCSAVSQDVVKVAGQQARALLRDRHHLRADQEDDFNIRNPEDIIQAQLEAS
                     RTLTLLLVAIASVSLIVGGIGIMNVMLVSVTERTREIGVRVSVGATEEAIQLQFLAES
                     VMLSLVGGAAGVLAGVVGSFFVGRALDWPMEMSLKAVLLAAVFSIAVGVFFGYYPARK
                     ASHLDPIEALRYE"
     gene            3479..4837
                     /locus_tag="VNN25_13765"
     CDS             3479..4837
                     /locus_tag="VNN25_13765"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH92644.1"
                     /translation="MSESSEASNGSSPLRRRKVWLIAGGIVILIGALSIPFLRRKESV
                     QYLASKVLQGEIRDAVEATGIVNAVVSVQVGSQVSGRVGKLNVDFNSRVRQGDVIALI
                     DPQLFQGALQQAQADLENAKANVIAAKASLTKANANLIQTRADFDRARSLAEKKVGTE
                     QALDQAKANYDAARANVDAAAASIAQANAQVSQKEAAVAVARTNLAYTVIRSPIDGTV
                     VARSVDVGQTVAASLQAPTIFTIAQDLAKMLVYAKVDESDVGRIRPGQTVTFKVDAFP
                     KQLFTGTVSQLRMNATTVQNVVTYDAIITFANPELKLFPGMTAYVTIPVATVENAVKL
                     PNAALRFQPPLPPAEVRALYAQYGIDDSKMGIDAAVEGSGAAAAANRGSGSRNALHDS
                     AVVWKLHADGTIEPVEIALGITDHAYTEVTKVFVGALKPGDDVMTTSVSSKSGPPGGI
                     RR"
     gene            4840..5586
                     /locus_tag="VNN25_13770"
     CDS             4840..5586
                     /locus_tag="VNN25_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92645.1"
                     /translation="MPNTNPILRVEDVHKYYELGETRVHALRGINVSVEPGGFVAIMG
                     ASGSGKSTFMNILGCLDRPTSGRYLLEGIDVSGRTKHELAGIRNSKLGFVFQGFNLLP
                     RTTALENVQLPTLYTPINAIERQKRAREALALVGLAARSQHYPSQLSGGQQQRVALAR
                     ALVNHPSILLADEPTGNLDSRTAVEIMEVLQDLNEQGLTIVLVTHEPDIAQFAKRMIV
                     FRDGKIRKDDVLRDRPRAHDVLLTMPTLED"
BASE COUNT         1069 a         1800 c         1745 g         1090 t
ORIGIN      
        1 gctcctgact cccgaggcca ttgagggagt gatcgatctg attccggaat cgtggctggg
       61 tgacgagccc cgctttgcca ccatcgccga gcatcgcctc gggtatcgtg actatctgat
      121 gcagcgcctg gcatcgccgc gcaagtggat ggaggacgcc gttcatgcac gaacgcttcg
      181 tgtttgacta cgccgtcgta cggatcgtcc cgcgcgtcga ccgcggcgag ttcctcaacg
      241 cgggggtcat cgtcttctca cccaccgccg gcttcctcga ctgccgcatc gagctcgatc
      301 acgcccgttt actcgcacta gccccatcga tcgacatcgc cgtcgtcgag agctatctcg
      361 acgcgatgcc caaaatctgc gccggcggcg gcgaagccgg ctcgatcggc gatctccccc
      421 aacgcgcccg attccactgg cttgtcgccc cgcgcagcac ggtgatccag acgtccgccg
      481 tgcactccgg cgttcacgaa gacccgcgag ccgcgctgga aggattgttc gagaagctcg
      541 tgcggacggt gtaggttcca ccgaggttca cgccttttcg cgcttgggct tcttcgttct
      601 gctcagcttc ggcagtagtt ttgtggcccg ctcgaaatca gcatccacag ggagtgaggc
      661 actggaagcg agcgctttga atttgtcgta ctccaactcg gccttcgtca gcgccatctc
      721 atgagagatg tgaccggcgt gagtgaggat gtcgcgctcg ttgaggctca ggaagccgtc
      781 cagcttcttg atccagtctc ccatgtgcat cggaacgcgg cgcattgcct gcccctcggc
      841 aaaaacgagg tattgctcga cgagattgtt cagcgcggca agttccggct cggtcagata
      901 gttcttggcg atcgcgacgt cgtccttgcg aatggttgcg ccgcgccagt tggtcagccc
      961 gaggttgggc ttggcggcgt cgacgcgctc gtgaacgatc tctgcggcag tctggcctgt
     1021 gatggcccag tgcaccttgt tctggaccgt tttgaagaag cggatgctga tgtcctgcgt
     1081 agggtcgtag tcgatgctgg tggcgtagat gtcggtgatc ttttggtaga agcgccgctc
     1141 cgaggtccgg atgtcctgga tccggcgggt cagttcttcg aagtaatcga acggctggtc
     1201 gggattcttc agccgctcat cgtcgagaac gaagcctttg acgatgtact cgcgaagacg
     1261 ctgggtagcc cacatccgga attgggtgcc tcggtgcgac ttcacccggt aaccgaccga
     1321 gatgatgacg tcgaggttgt agaacctggt cgagtagttt ttcccgtcgg cggcagttgt
     1381 caaggaatcc ttgacaactg aatcctcggc taactcgcac tcagaaaata cgttcaggat
     1441 gtgaaggctg acgttctgct tggtagtctg gaagagttcc gccatctgct gctgcgtcag
     1501 ccagacggtc tcgccttcga agcggacgtc aatcttcagc ttcccgtcct gggtctgata
     1561 gacgaggaac tgtccacgct gaggagtcat gggaggcgat catgcctttc accgatcaat
     1621 gattcaacga gtttgaccgg ccgctctcga agctcaaggc ttgaatcagg gagcaatctt
     1681 cacgtggtag atgtcggcta aggattttcc tccgctcctc cgcgtctccg cgtgaaatcg
     1741 ttttacagtg ccgccacgat gacagacatt ctcttcactc cgcacccaat cggcttcgcg
     1801 cggactccgt tcaaggacac cacgcaggtc ccgaaaggtc cggggaccaa gcatgaagct
     1861 gacggcacgc tcgagatcct gcctgagttt gcgcagggcc tgaaagacat tgaaggcttc
     1921 tcgcacctat tcgtgatctg ggtgttcgat cgctcggacg gttacgacct tctcggcgct
     1981 ccgcccggag aaacggtcca gcacggagtc ttcgccaccc gctctccgcg ccggccgaat
     2041 ccgatcgcgc tcacggtcgt cgagctgctg cgccgcgaag ggaccacgct acacgtgcac
     2101 ggcgtcgaca tgctcgaccg aacgccaatc ctcgacatca agccgtacat gtcgagtatt
     2161 ccgatcgaca agctgcggcg cgggtggctg gaggatacgg agcggcgcgc gggcaagagt
     2221 taacgcccct gttaactgcg cttcgactac agtgcgcggc ctgaaacgat gaactacctg
     2281 gcactgctca ccagttcctt gcgcgcgctc ctacggaaca agatgcgcag cttgctcacg
     2341 gtgctcggca tcaccatcgg aatcgccgcc gtcatctgcg tggtcgcgat cggcagagcg
     2401 ggccaagtgc gcgtcgagca gcagctcaac aacctcggcg acaacttcgt ctggatcgag
     2461 gcgggagggc gcgcggtcaa cggcgtgcgc accggcccgc gcggcacgaa gacgctcacg
     2521 attggcgatg ccttcgccat ccgcaaccag attccgctca tcaagagcgt ctcgccgaac
     2581 gtcgatgcgt caacgcaggt catctacggc aaccagaact ggttcacgag ctatcgcggc
     2641 gtctcgccgg agtacttcga catcaagcgc tgggtgatcg atgaaggggc ggcgttcacg
     2701 gccgacgacg tcgaacgcgc ggcggacgtc tgcgtgatcg gcgcgaccat ccgcagccag
     2761 ttgttcggcg tggccaatcc gatcggccag gtcatccgcc tcaaatcgct tccctgcaaa
     2821 gtcgtggcga cgctgaaacc gaaaggcctg tcgatctccg gccaggatca ggacgatacg
     2881 atccttcttc cctacaccac cgcgcagaaa aaactggcgg gcgtgacctg gcttgacgac
     2941 atcctctgct cggcagtctc gcaggacgtc gtgaaagtgg ccggacaaca ggcgcgcgcc
     3001 cttctccgcg accggcatca ccttcgcgcc gatcaggagg acgacttcaa catccgcaat
     3061 cccgaggaca tcattcaggc gcagctcgaa gcgagccgca cgctcacgct gctgctggtg
     3121 gcgatcgcct cagtctcact gatcgtcggc ggcatcggaa tcatgaacgt gatgctcgtc
     3181 tccgtcaccg agcgcacgcg cgagatcggc gttcgcgtgt cggttggagc gacggaggaa
     3241 gcgatccagc tccagttcct ggcggagtcg gtgatgttga gcctcgtcgg aggggcggcc
     3301 ggcgttctgg ccggcgtggt cggctcgttc ttcgtcggac gcgcgctcga ctggccgatg
     3361 gagatgtcgc tgaaggccgt gctgctcgcg gcggtcttct ccatcgccgt cggcgttttc
     3421 ttcggctact atcccgcgcg caaggcgtcc cacctcgatc ccatcgaagc cctccgctat
     3481 gagtgaatcc tccgaagcat ccaacgggag ctccccgctc cgccgccgta aggtgtggct
     3541 gatcgccggc ggcatcgtga ttttgatcgg cgcgttgtcc attcctttcc tccggcgcaa
     3601 agagagcgtt caatatctcg cttccaaagt gctgcagggc gagatccgcg acgcggtcga
     3661 agcgaccggt atcgtgaacg cagtagtcag cgttcaggtc gggtcgcaag tctcgggcag
     3721 agtcggcaag ctcaatgtcg atttcaattc gcgcgtgcgc cagggcgacg ttatcgcact
     3781 gatcgatcct cagttgtttc agggcgcgct gcagcaggcg caggcggatc tcgagaacgc
     3841 gaaggcgaac gtcatcgcgg cgaaggccag cctgaccaag gccaatgcga atctcatcca
     3901 gaccagggcc gacttcgacc gcgcacgatc gctggccgaa aagaaagtgg ggaccgagca
     3961 ggcgctcgat caggcgaaag cgaactacga tgctgccagg gcaaacgtcg acgccgctgc
     4021 agcgagcatc gctcaggcca acgcgcaagt cagccagaag gaagcggcgg tggcggtggc
     4081 ccgaacgaat ctcgcctaca ccgtgatccg ctccccgatc gacggcacgg tcgtggcccg
     4141 cagcgtcgac gtcggccaga ccgtggccgc ctcgctgcag gcgccgacga tcttcaccat
     4201 cgcgcaggat ctcgccaaga tgctcgtcta cgccaaagtc gatgagtcgg atgtcggccg
     4261 catccgccct gggcagaccg tgacgttcaa ggtcgatgcg tttccgaagc aactgttcac
     4321 cggaaccgtc agccagctcc ggatgaacgc caccaccgtc cagaacgtcg tcacgtacga
     4381 cgccatcatc acgttcgcaa accccgagct gaagctcttt cctggaatga cggcgtacgt
     4441 cacgattccg gtggccaccg tcgagaacgc ggtcaagttg ccgaatgcag cgctacgttt
     4501 tcagccgccg ctcccgcctg ccgaggtccg cgcgttgtac gcacaatatg gaatcgatga
     4561 ttcgaagatg gggatcgatg ccgctgttga aggcagcggg gcggctgcgg cggcgaaccg
     4621 cggcagtggc tccaggaacg ccctgcacga cagcgcggtc gtctggaagc ttcacgccga
     4681 tggcaccatc gagccggtcg agatcgccct cggcatcacc gatcatgcgt acaccgaggt
     4741 gacgaaggtg ttcgttggag cgctgaaacc gggagatgac gtcatgacca cgtcggtcag
     4801 ttcgaagtcc gggccgcccg gcggcatcag gcggtgacgg tgcccaacac taacccgatc
     4861 ctccgcgtcg aggatgtcca caagtactac gagctcggag agacgcgcgt gcatgcgctg
     4921 cgcggcatca acgtctcggt cgagccgggc ggattcgtcg ccatcatggg cgcgagcggc
     4981 agtggcaagt ccaccttcat gaacatcctc ggctgcctcg accggccaac ctcagggcgc
     5041 tatctgctcg aaggcatcga tgtgtcgggc cgcacgaagc acgagctggc aggcatccgc
     5101 aactccaagc tcggattcgt gtttcaggga ttcaatctcc ttccgcgaac gacggcgctc
     5161 gagaacgtgc aattgccgac gctctatacg ccaatcaacg ccatcgagcg acagaagcgc
     5221 gctcgcgagg cgctggcgct cgtcggcctg gccgctcgat cgcagcacta tccgtcgcaa
     5281 ctctcgggag gtcagcagca acgcgtcgcc ctcgcacgcg cgctcgtgaa ccatccatcg
     5341 atcctgctcg ccgacgagcc gaccggaaac ctcgacagcc gcaccgcggt cgagatcatg
     5401 gaggttctgc aggacctgaa cgagcaggga ttgacgatcg tgctggtcac gcatgaaccc
     5461 gatattgcgc aattcgcgaa acggatgatc gtctttcgcg acggaaaaat ccgaaaggac
     5521 gatgtgctgc gcgaccgccc gcgagcgcat gacgtgctgc tgacgatgcc gacgttggag
     5581 gattagcgcc ggagattagc cctgcttcgc gctaggttcg tcgctcttct cgaacctctt
     5641 cgcaccactc tccaatcgag cgccttctcc ccccgcgctt ttgtggggga gaaggtggcc
     5701 gaag
//
LOCUS       DATLVW010000453        12750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_390152, whole genome shotgun sequence.
ACCESSION   DATLVW010000453 DATLVW010000000
VERSION     DATLVW010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390152"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..783)
                     /locus_tag="VNN25_13775"
     CDS             complement(64..783)
                     /locus_tag="VNN25_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92646.1"
                     /translation="MHQQPLVRLTEITRTYPLGRVQVRALTGVSLDIARGDFLALAGP
                     SGSGKTTLLNIIGCIDKPDGKPDSGRVVIDGIDVTEVPLHRLAATRRDTLGFIFQTFN
                     LIPVLTAFENIEYPLLLQHLPARQRRERVRYWLDQVGLSDQARQRPDQLSGGQRQRVA
                     IARAMAGEPKLVLADEPTANLDSVTAARILDLLEGLNDSTGCTFIFATHDPALMSRAR
                     RCVRMHDGAIVSEPLLEARAS"
     gene            961..1230
                     /locus_tag="VNN25_13780"
     CDS             961..1230
                     /locus_tag="VNN25_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HXH92647.1"
                     /translation="MATTKKSPGKGLAAEVTPSSDLATIVGSKPIPRSEVTKKVWDYI
                     KANGLQDKTNKRMINADDKLLKIFNGKKQVSMFEMTALVSKHLKA"
     gene            complement(1283..1690)
                     /locus_tag="VNN25_13785"
     CDS             complement(1283..1690)
                     /locus_tag="VNN25_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92648.1"
                     /translation="MRALCSIAAVLLMLLNAGCHSPTSPDNGMPAQHGRLSGIVTIGP
                     NCPGPSANPCPTPPSAYAARKILVYTETKSNLLFTVDIDSQGAYFIDLAPGRYTIDLK
                     PSGIDKTSDLPKLVEIHANTVTSLNVSIDTGIR"
     gene            1751..2233
                     /locus_tag="VNN25_13790"
     CDS             1751..2233
                     /locus_tag="VNN25_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXH92649.1"
                     /translation="MKRLPSIGVLLVLLVLAACSSSAPGSAGVTAGDAENAIRHANSQ
                     LSADVRAGNAQAIVDNFYAPDAVAMAPNVPAMHGRDAIRAFWTQVLGSGAVELALTSN
                     HVTQSCSDLAVENGRYDLTITPKGGQAIHDTGKYIVVWTKAAGRWWATEDIFNSDMPK
                     "
     gene            complement(2445..3158)
                     /locus_tag="VNN25_13795"
     CDS             complement(2445..3158)
                     /locus_tag="VNN25_13795"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HXH92650.1"
                     /translation="MRRIGFLLLLLVAGIARASAPDERCPARTDITGLPVVTLDAKVP
                     SDRFAVMITGDGGWRRIDRKITDRLRAAGIPIVGFIASDYYRTRRTPDESACDLERII
                     RYYRIQWKRNKVLLIGYSRGADVLPFMASRLPSDLRAVTQLIALLGLEPMIDFKYSPA
                     WTLNAYTHKEPKHAVKPDVEKLRGQNVLCIFGAKEKDSLCHQLDPRAFKIVAEPGGHH
                     FAGKYRDIADVILIESAGR"
     gene            complement(3155..3826)
                     /locus_tag="VNN25_13800"
     CDS             complement(3155..3826)
                     /locus_tag="VNN25_13800"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HXH92651.1"
                     /translation="MKTLMVLLLLARAASLSASTPKLPLVEVPATRGASDTLVVFVSG
                     DGGWAAIDKEISKVLAGEGMPVVGLNALQYFWTKRSPDSASADLRAIIDQYLSQWHKS
                     RVLLVGYSRGADVLPAMISRLPAETQARIRMIALLGPSPKVEFEFHVADWMRSGSAGE
                     AVMPEIGKLGAHRILCLSGEDDHESLCRELTGRPYVDVITLKGAHHFDGGYGNLGRII
                     IDHLK"
     gene            complement(3889..5187)
                     /locus_tag="VNN25_13805"
     CDS             complement(3889..5187)
                     /locus_tag="VNN25_13805"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH92652.1"
                     /translation="MALEGTLKDFSLADIFQLIGLQRKTGVLTLRSKDDTVTVTFLDG
                     KVVGADSLSHRLENRLGHVLLRSELLTQDQLNRALEIQKETLQRLGFILVHYGIISID
                     SLKQALQLQILQIVFRLFRWKDGDYHFSQETTIEYDRDYVVPVSAESILMEGARMIDE
                     WPIIEKRIRSYDMVFRKKMTDQEIVVAGASEEADEVDFDAAPTRRRKPAMTDKVRISR
                     DEKTIYDMVDGTTSVRGVMELSKLPEFDTAKALYELLTRDLIEEVRGSSAAEVLAHMT
                     PVDEDEVAETPVPLPLVAVLALLALLSLATSFRNPLNSLHPLGPAHRSSSIAETRKAI
                     SIERIEQIGESLEMYNVINGRLPARLQDLVPLYLSPSLLRDPWAHAYKYLPQPQRYLV
                     IGFTPDEKPDTDLFLSHVIDAGAPSAASSKPVTGGIQLID"
     gene            complement(5200..7044)
                     /locus_tag="VNN25_13810"
     CDS             complement(5200..7044)
                     /locus_tag="VNN25_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92653.1"
                     /translation="MRQRLISLLAASMCLASVAAHAGDTAPRDIWSQAAASADAGNID
                     AAAKKTSELMDVGRASGIRTYPSYAAAAAILARQAAKQSNKQVADWANKAADTLDPGS
                     SSVAFIRAEEAVESGSWGTALPVLVRGIAGLFARYRSRLLARADTVITLLLALALTAA
                     IFSFALLIRYGRAMTHDFREMLGERLHGGSVSVLAFALLFLPVFLWLGPLWLVFYWFI
                     IFFGYAKIAERIMIVVLSLLLAGAPLALDWTSQRIAGIDSPVVMSAITSDERTYEPDA
                     LRRMQELVNIVPDDAVLHLLLGTLYLQEGNDQQASQHYQRSAELHDNAGAHVNLGNLH
                     FYNNDFATAVTEYNKAEALDPRLAIAFYDESIALGELYKFDQQAQKLDVARRIDRALV
                     ERSSEPRAQKLEVVLYHPSIPQAWAVSSEIARRGIARSLFGNYAWFDPLESMRNPLTA
                     GAILTLLLAPLLFLKRRRAGFAGSCFKCGRTFCLRCKSARESTTFCTQCIHIYLKRDG
                     VSAATKRAKLEEVSEHQQGILKRNRWLATFLPGSAQVIEGHTAGGTVASFAFAFFVSL
                     AILSGRLAPVLAPGDTAKTIVRIAAIVFAAALWLFITLPVYRRNINSF"
     gene            complement(7041..7544)
                     /locus_tag="VNN25_13815"
     CDS             complement(7041..7544)
                     /locus_tag="VNN25_13815"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXH92654.1"
                     /translation="MERPKSEIKVTDKRIFTAEGEIREEFKQDVTPVDPAAAKPAAAP
                     AATESEKPQPEASKAPSPARAAEPPTGDRRNKSVADKALNPGTQFADFVEPLIAQAYM
                     SLGMLRNPYDPKPKLDAAAARQMIEMLTMLKEKTNGNLTADEDDFLSTHLGELKLAFV
                     QRTKSIT"
     gene            7671..8780
                     /locus_tag="VNN25_13820"
     CDS             7671..8780
                     /locus_tag="VNN25_13820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH92655.1"
                     /translation="MRIFQILEFNQFNTGSVHQMFQAASGLRERGHDVTIISRPDAVL
                     EEKAHQAGVAFAGLHMRNEFDLASAWKLGHLFREQRPDVVHVHKGVAHALALAATFAR
                     PVGAFIVNRGVSFPLDVWNRIKYRTRRVDRVVTVCAQIQDVIIASGRLPREKVVVVYA
                     GTDVRLFDPARWDSTAFRREKGIADDAFLIAQVGVRGWKGWRELIDAVASILPTHPHA
                     HLVLIGCRDESERGEVASYAHQRGIGPHVTPVEYRDDMPNVFASCNLVVDASWAGTGI
                     TGTIREAMAMRKPVIATDCGGNRELVTSADVGWLIPAKEVPPLARAIAEVIDDPARRE
                     RIAANAREHVVQGFSKDLRITRLEALYDSVLRIEG"
     gene            complement(8849..9784)
                     /locus_tag="VNN25_13825"
     CDS             complement(8849..9784)
                     /locus_tag="VNN25_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXH92656.1"
                     /translation="MISIYTNPVFYKHDTGVGHPETAKRLDAALAGVARAGLAGRITR
                     ETAEHHDTNRLIAKVHSADYERSLEEASRSGMRLFHSIDNPMSSQTFAAARTAVGTAL
                     TAAEEMMQSEAGRAFVVARPPGHHAERLEAMGFCFFNTIACVAEWIREQPGMDRVFIF
                     DFDVHHGNGTQSLFWDRDDVFYLSMHRFPFYPGTGAASEIGSGAGRGTTMNIPLQGGL
                     GDAAYLRSVEDVVVRAIDAYQPQAILLSSGFDAHRRDPLGGMRVTEQAYGEITRRVVE
                     AAGRHSGGRVLSLLEGGYDMEGLAASVAEHVNALA"
     gene            10089..>12750
                     /locus_tag="VNN25_13830"
     CDS             10089..>12750
                     /locus_tag="VNN25_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92657.1"
                     /translation="MVTTDGMAYAKNMVFWTGRLTAIVVDPTAPATMYLGAAKGGVWK
                     TINGGKSWSPMSDDELSLAIGAIALDPVTPQTVYAGTGEGNIFYVVNVPDSTQESYYG
                     AGILKSLNGGASWNLYGQAEFTGAAFFRIAIHPTNPNRVWAATSNGLFLSDSGGTSWT
                     HVTNGLPPTVPGTATGCTEIVLHPSDPSIAFTYFIGEGAYQCTNANTASPGFTPVPDL
                     QAGNKFRASIAFSPSQPQRMVAGFDDDLWYSTDGGQTWKPTGVPLVKCTITFYCINVA
                     IDPTDPNVVYLSGEPQIMKFTFDPVGKTWSGSNIGLGKTHDDHHALAFDPTNHLHIYA
                     GTDGGIYESHDGGGSWSDDLNRGLSITQFDFIDHHPGSDAVVFGGTQDNGTQQFRNST
                     VFPEVSSGGDGGCVAVDFNNPHNVVIEYFHISPARSTDAGEYATFTDISPGLFGSTLF
                     YPPYALCAEDPTWIAFGSTGIFIDKAQGTGGWPTLVKLPGITGPISAINFVSKILIYC
                     ATIHGEVYAIRSTNGGVDWTPTAIHAAPFPLRFVWDIGVMPNDSAHIIAVVSGYGSGH
                     VWSAIVPPNGVAVWTDVSGSGASGFPDVPANSLVIDPAAPSTYYVGTDIGVFKTTNAG
                     GMWSNFGQGLPNTAVFDLRLHGPSQLLRAATHGRGIWERRVDVASMPDVDIYVRHHMM
                     DTGRGLAPEGVPAAFKNVGQFVGLGDPQRHWMCVDMKVDALLGNPQSFQYAPSEVDFV
                     VYEAQLYHRDAERGNVNRVYAQVHNRGIKASNVTVKLLFADATAKVPPLPNDFWSAFP
                     GNSAVPNSPWHAIGAAKTVSVTPTLPTVLEWDWATPMGQATHTCLLLIVNSQDDPIPA
                     AHKIFDVDALVKLDKRAGLKNLHVVNAPPKP"
BASE COUNT         2466 a         3928 c         4011 g         2345 t
ORIGIN      
        1 agcgccggcg gttgcgcaag acgttgcgaa gggcaagttt ggccagcatt ggtggttttg
       61 tccttacgaa gcacgcgctt cgagtaatgg ctcggagaca atcgccccgt cgtgcatgcg
      121 gacgcagcgt cgtgcgcgcg acatcagcgc cgggtcgtgc gtagcgaaga tgaacgtgca
      181 tcctgtggag tcgttgagtc cttcgagaag gtcgaggatt cgcgcggcgg tgactgagtc
      241 gaggttggcg gtcggctcgt cggcaaggac gagtttcggc tcgccggcca tggcacgagc
      301 gatagccacg cgctgccgtt gcccgccgga gagctgatcg ggacgctggc gcgcctgatc
      361 cgacagccca acctgatcga gccagtaacg aacgcgttcg cggcgctggc gcgctggcag
      421 atgctgcagc agaagcggat actcgatgtt ctcgaacgcg gtaaggacgg ggatcaggtt
      481 gaaggtttga aagatgaagc cgagggtgtc gcgccgcgtc gcggccagac ggtgcagcgg
      541 gacctcggta acgtcgattc cgtcgatcac gacgcggccg ctatcgggct taccgtctgg
      601 tttatcaatg catccgatga tgttgagcag cgtcgttttg ccgctgcctg aaggaccggc
      661 caacgccagg aaatcaccgc gggcaatgtc gagcgagact ccggtaaggg cgcggacctg
      721 gacgcgcccg agggggtatg tgcgggtgat ttctgtcagc cggacgagtg gttgctggtg
      781 catgggcatc tccttcgcag accaatctag aggcccgcga acggatgcac atgtgcctga
      841 agcgcggcag gctcatcatg acttttggca catcgaccga aattccccgt ttgacgcgca
      901 gggtgcagta gcgcacaatc cccgaaaacc tggaacatta cgtaggagag gacattacta
      961 atggcgacaa cgaagaagag ccccggcaaa ggtcttgccg cggaagtaac cccgagcagc
     1021 gacctggcca cgatcgtcgg cagcaagccg atcccccgca gcgaagtcac gaaaaaggtg
     1081 tgggactaca tcaaggccaa cggccttcag gacaagacga acaagcggat gatcaacgca
     1141 gacgacaagc tgctgaagat cttcaacggc aagaagcagg tctcgatgtt cgagatgacc
     1201 gctctcgtca gcaagcacct gaaagcgtag tcccggaacc gcgagccatt cgaggccggc
     1261 gttctgcgcc ggccttctcg tttcagcgga tgcccgtgtc gatgctgacg ttcaggctcg
     1321 tgaccgtgtt cgcatggatc tcgacgagct tcggcagatc ggacgtcttg tcgatgccgc
     1381 tcggcttgag gtcgatcgtg tatctgcctg gcgcaagatc gatgaagtag gcaccctgcg
     1441 agtcgatgtc gaccgtgaag agcaggtttg atttcgtctc cgtgtagacg aggatctttc
     1501 gcgcggcata ggccgacggc ggcgtcggac agggattcgc ggaaggaccc gggcaattcg
     1561 gcccgatcgt gacgatgccc gagagtcgcc catgctgcgc cggcattccg ttgtctggcg
     1621 acgtcgggct gtgacagccg gcgttgagca gcatcaacag gaccgcggcg atcgaacaga
     1681 gagctcgcat cgcgcgtatt ctagatccgc cggcccgccg gggtcgtagt tcattcgcaa
     1741 gggggatttc atgaaacgac tcccatcgat cggagtgctc ctggtcctgc tggttctcgc
     1801 cgcatgttca tcgtcggcac cgggatcggc cggcgtcacg gcgggcgatg cggagaacgc
     1861 catccgccac gcgaacagtc agctttcggc agacgtgcga gccggaaacg cgcaagcgat
     1921 cgtcgacaac ttctacgctc cggacgcggt cgcgatggcg ccgaacgttc ccgcgatgca
     1981 cggccgcgat gcaatccgcg cgttctggac gcaggttctc ggcagcggcg cggtcgagct
     2041 ggccctgacg tccaatcatg tgacgcagtc atgcagcgat ctggccgtcg aaaacggccg
     2101 ctacgacctg accatcacgc cgaaaggcgg ccaggccatt cacgataccg gcaagtacat
     2161 cgtggtgtgg acgaaggctg ccggccgctg gtgggccacc gaggacatct tcaattcgga
     2221 tatgccgaag tagggggtgg atgtcgggag tggggggtgg gggtgcagag ataagggatc
     2281 aggggatcag agacaagaac gcagagagca acgccgaaga caaggggtca gagacaggag
     2341 atcaaggaca gggggaacca gagactcgtc tgatctcttg tctctgattg tctctgatct
     2401 ctggtctctg ctctcccacc ccccaccccc tactccccac cccctcaccg ccctgctgac
     2461 tcgatcaaaa tcacatccgc gatgtcgcgg tatttgcccg cgaagtggtg tccgccgggc
     2521 tccgcgacga tcttgaatgc tctcgggtcg agctggtggc agagcgagtc tttctccttc
     2581 gctccgaaga tgcacagcac gttctgaccg cgcaacttct cgacatcggg tttcacagcg
     2641 tgtttcggtt ccttgtgcgt gtacgcgttg agcgtccatg ccggcgaata cttgaagtca
     2701 atcatcggct ccaatccgag gagggcgatg agctgggtga cggcgcgaag atcggacgga
     2761 agacggctag ccatgaacgg cagcacgtcg gcgccacgcg agtagccgat caacaacact
     2821 ttgttccgct tccactgaat ccggtagtag cggatgattc gctcgaggtc gcaggccgac
     2881 tcgtcgggcg tacgccgcgt gcggtagtag tcggaggcga tgaatccgac gatcgggatg
     2941 cccgcggcgc gaagacggtc cgtgatcttg cgatcgatgc gccgccagcc gccgtcgccg
     3001 gtgatcatga cggcgaaccg gtcgctgggg actttcgcgt cgagcgtcac caccggcagg
     3061 ccggtgatgt ccgttcgcgc cgggcagcgc tcgtccggtg cgctggcgcg cgcaatgccg
     3121 gcaacgagaa gcagtagaag gaatccgatt cgtctcattt gaggtgatcg atgatgatgc
     3181 ggccaaggtt gccatagccg ccgtcaaagt ggtgtgcgcc cttgagcgtg atgacgtcga
     3241 cgtagggacg gccggtcagc tcgcggcaga gcgattcgtg atcgtcttcg cccgacaggc
     3301 agaggatgcg atgcgcaccg agtttgccga tttccggcat gaccgcttct cccgcggagc
     3361 cgctgcgcat ccagtcagca acgtgaaact cgaactccac tttcgggctg gggccgagca
     3421 gcgcgatcat acggattctc gcctgcgtct ccgcaggcag ccggctgatc atggccggca
     3481 gaacgtccgc accgcgggag taaccgacca ggagcacgcg cgatttgtgc cattggctga
     3541 gatattgatc gatgatcgct cggaggtcgg ccgaggcgct gtcgggagag cgtttcgtcc
     3601 agaagtactg cagcgcgttc aggcccacca ccggcattcc ctcaccggcg agaaccttcg
     3661 aaatctcctt gtcgatggct gcccacccac catcaccgga aacgaaaacg accagcgtgt
     3721 cggacgcgcc gcgtgtggcg ggcacctcga ccagcggaag tttcggcgtc gatgcggaga
     3781 gcgatgcagc ccgggcgagc agcaacagaa ccatgagtgt cttcatacgg accggcgggc
     3841 agaacaacgc acgcccattt cgtattcgtt agttaagtta gccgattatt agtcgatgag
     3901 ttgtatcccg cccgtcaccg gcttcgagga ggccgccgaa ggtgctccgg cgtcgatcac
     3961 atgcgagagg aagaggtccg tgtcgggctt ttcgtcggga gtgaatccga tgacgaggta
     4021 acgctgcggt tgggggaggt atttgtacgc gtgagcccac ggatcgcgca gaagcgacgg
     4081 cgagagatag agcggaacaa gatcctgaag cctcgccgga aggcgtccgt tgatgacgtt
     4141 gtacatctcg agcgactcgc cgatctgctc gatcctctcg atggagatcg ccttgcgcgt
     4201 ttccgcgatg gacgacgatc gatgggccgg gccgagcgga tgcagcgagt tgagcggatt
     4261 ccggaacgat gtggccagcg aaaggagcgc gaggagcgcc agcactgcca cgagcggcaa
     4321 cggcacaggc gtctcggcga cttcgtcctc atccaccggt gtcatgtgcg cgagaacttc
     4381 cgcggcactg cttccgcgca cttcctcgat caggtcgcgc gtcaggagct cgtacaacgc
     4441 ctttgccgta tcgaactcgg gcagcttcga cagctccatg acgccgcgca ccgatgtcgt
     4501 gccgtcgacc atgtcgtaga tggttttctc gtcgcgcgag atgcggacct tgtcggtcat
     4561 ggccggcttg cgccggcgcg taggcgcggc atcgaagtcg acctcatccg cttcttccga
     4621 cgcgccggcg acgacgatct cctgatccgt catcttcttg cggaagacca tgtcgtacga
     4681 gcggatgcgc ttctcgatga tcggccactc gtcgatcatc cgcgcgcctt ccatcaggat
     4741 cgattcggcg ctgacgggaa cgacgtaatc gcggtcgtac tcgatcgtcg tctcctgcga
     4801 gaagtgatag tcgccgtctt tccatcggaa gaggcggaag acgatctgga gaatctgaag
     4861 ctggagcgcc tgcttcagcg agtcgatcga gatgattccg tagtgaacga ggatgaatcc
     4921 aagccgctgg agcgtctctt tctgaatctc cagcgcgcgg ttcagctggt cctgggtcag
     4981 cagttcgctt cgcagaagca cgtgaccgag ccggttctcg aggcggtggc tgagcgagtc
     5041 ggcaccgacg accttcccgt ccaggaacgt caccgtgacg gtgtcatcct tgctgcgaag
     5101 cgtcagaact ccggtctttc gctgcaggcc gatgagctga aagatatcgg cgaggctgaa
     5161 atctttgagt gtgccttcga gagccatagc aaaccatcat caaaacgaat tgatgttcct
     5221 ccgatagaca ggcagcgtta tgaacaacca gagcgcggcg gcgaagacga tggcagcgat
     5281 gcggacaatc gttttggccg tatccccggg cgccagaacc ggcgcaagcc tgccggagag
     5341 aatcgcaagc gaaacgaaga acgcgaatgc gaacgacgca acggtgccgc cggccgtgtg
     5401 tccctcgatt acctgcgccg agccgggcag gaaggtggcc agccatcggt tccgcttcag
     5461 gatgccctgc tgatgctcgc tgacttcttc gagcttggcg cgtttcgtgg ctgctgaaac
     5521 accgtcccgc ttcaggtaga tgtgaatgca ctgcgtgcag aaagtcgtgc tctcccgcgc
     5581 cgacttacaa cgaagacaga aggttcgccc gcacttgaag caggatccgg cgaatccggc
     5641 gcgccgccgt ttgaggaaga gcagcggcgc cagaagcaag gtgagaatcg cgcctgccgt
     5701 cagtggattg cgcatgctct ccagcggatc gaaccaggcg tagtttccga agagcgagcg
     5761 ggcgatccct cggcgggcaa tctccgagga gacggcccaa gcctgtggga tgcttggatg
     5821 atagagaacg acttcgagct tctgcgcccg cggttccgag gagcgttcga cgagagccct
     5881 gtcgattctc cttgccacat cgagtttctg cgcctgctgg tcgaacttgt acagctcccc
     5941 gagggcgatg gactcgtcat aaaacgcgat cgccagccgc ggatccagcg cctcggcttt
     6001 gttgtactcc gtcaccgcag tcgcgaagtc gttgttgtag aaatggaggt tgccgaggtt
     6061 gacgtgcgct ccggcgttgt cgtgaagctc cgccgagcgc tggtagtgct ggctcgcctg
     6121 ctgatcgttg ccctcctgaa ggtaaagagt gccgaggagg agatgcagca cggcgtcgtc
     6181 agggacgatg ttgacgagct cctgcatccg ccggagcgcg tccggctcat aggttcgctc
     6241 gtcgctggta atggcagaca tcacgaccgg actgtcgatc ccggcgatcc gctgcgatgt
     6301 ccaatcgagc gccagaggcg cgccagccag caacaatgag aggacgacga tcatgatcct
     6361 ctcggcgatc ttggcgtaac cgaagaagat gatgaaccag tagaagacga gccagagcgg
     6421 tccgagccag aggaagaccg gcagaaaaag aagagcgaac gcaaggaccg aaaccgagcc
     6481 accgtgcagc cgctccccga gcatttcgcg gaagtcgtgc gtcatggcgc ggccgtaacg
     6541 gatcaataga gcgaacgaga aaatcgcggc cgtgagcgcg agagcgagaa ggagcgtgat
     6601 caccgtgtcg gcacgggcga gcagccgcga acgatagcga gcgaagagac cggcgatccc
     6661 gcggacgagt accggaagcg ccgtgcccca gctccccgat tccaccgcct cctcggcgcg
     6721 gatgaacgcg accgaggacg agccgggatc gagggtgtca gcagccttgt tcgcccaatc
     6781 ggcaacttgc ttattcgact gctttgccgc ctgacgcgcc aggatcgccg cagcggcagc
     6841 atacgaagga tacgttcgga tgccggacgc ccggccgaca tccatcaact cgctggtctt
     6901 ctttgcagca gcgtcgatat tgccggcgtc cgcggacgcg gcagcctgcg accagatatc
     6961 gcgaggcgct gtgtctcccg cgtgtgccgc gactgacgcg aggcacatcg acgcagcaag
     7021 cagcgagatc agacgttgtc tcatgtaatg cttttcgtcc gttggacgaa tgcgagcttc
     7081 aattctccca gatgcgtcga caggaaatcg tcctcatccg cggtcaggtt cccgttggtc
     7141 ttctccttga gcatggtcag catctcgatc atctgacggg ccgcggcggc atccagcttc
     7201 ggtttcggat catacgggtt gcgcaacatg cccaacgaca tgtacgcctg cgcgatgagc
     7261 ggctcgacga agtccgcgaa ttgtgtgccg ggattcaacg ccttgtctgc caccgacttg
     7321 ttgcgccggt cgccggtggg cggctcagca gcacgggctg gagaaggagc cttcgaggct
     7381 tccggctgcg gcttctcact ctcggttgcg gcaggggccg cagccggctt cgcggcagcc
     7441 ggatcgaccg gcgtgacgtc ctgcttgaac tcttcgcgga tctccccttc agcggtaaag
     7501 atccgcttgt ccgttacttt gatttcggat tttggtcgtt ccatttggct cttggcgagc
     7561 ggccagcctg cgtgctggat tctgtgcgcg aagctccttt caagggaccg gaaacgatat
     7621 agcaagtgcg cggttgcgcg ctaccgccgg tacaatccgc ccccttgccg atgcgcatct
     7681 tccagatcct cgaattcaat caattcaaca ccggctccgt gcaccagatg tttcaggcag
     7741 cgtccggttt gcgcgagcgc ggtcatgacg tgacgatcat cagccgcccc gatgccgtgc
     7801 tcgaggaaaa ggcgcatcag gcgggggtcg ccttcgccgg gctgcacatg cgcaatgagt
     7861 tcgatctcgc cagcgcgtgg aagttgggac atctttttcg ggagcagcgg cccgacgtcg
     7921 tccacgtgca caagggggtc gcgcacgcgc tggcactggc cgctaccttc gcccggccgg
     7981 tgggtgcatt catcgtcaac cgcggcgtct cctttcctct cgacgtctgg aaccgcatca
     8041 aataccggac gcgtcgcgtc gatcgcgtcg ttaccgtctg cgcgcaaatc caggacgtga
     8101 tcattgccag cggccgtctt ccgcgggaga aggtcgtcgt cgtctatgcc ggaaccgatg
     8161 ttcgtctctt cgatccggcg cgctgggact cgacagcctt ccgccgcgaa aaaggaatcg
     8221 cggacgacgc cttcctcatc gcgcaggtcg gcgtacgagg ctggaagggt tggagggagt
     8281 tgatcgacgc cgtcgcttcg atccttccca cccacccaca cgcccacctt gtgctgatcg
     8341 gctgtcgcga cgagtcggag agaggagagg tggcgagcta cgcacatcag cgcggtatcg
     8401 gaccgcacgt cacgccggtc gagtaccgcg acgacatgcc gaacgtcttc gcctcctgca
     8461 atctcgtcgt cgacgcttcg tgggccggca ccggcatcac cggcacgatc cgcgaggcta
     8521 tggccatgcg aaaaccggtc atcgccaccg attgcggcgg caaccgcgag ctcgtcacgt
     8581 cagccgatgt aggttggctg atcccggcga aggaagtgcc gccgctggcg cgtgcgatcg
     8641 cggaagtcat cgacgatccg gcgcggcgcg agcgcatcgc ggcgaatgcg cgggagcacg
     8701 tcgtgcaagg cttctcgaaa gacttgcgga tcacgaggtt ggaagcgctg tatgactcgg
     8761 tgcttcgaat agaaggctga aagcttttca cgcgaagaca cgaagaaaac cgaacttcgc
     8821 gttttcgcgt cttcgcgtga gaagcctgct acgcgagagc gttgacatgc tccgccacgc
     8881 tcgctgcgag cccttccatg tcgtagccgc cttccagcag agagaggacg cgcccaccgc
     8941 tgtgccgccc tgccgcttcg acgacacgcc gcgtgatctc gccgtacgcc tgctcggtca
     9001 cccgcatgcc tcccagcgga tcgcggcggt gtgcatcgaa ccccgacgac agcagaatcg
     9061 cctgtggctg atacgcatcg atggcgcgga cgacgacgtc ttcgacgctg cgcagatacg
     9121 cggcatcgcc gaggccacct tggagcggaa tgttcatggt ggtgccgcgg cccgcgccgg
     9181 agccgatctc gctagcggcg cccgttcccg gatagaacgg aaagcggtgc atcgagaggt
     9241 agaaaacgtc gtcgcgatcc cagaacaacg actgcgttcc gttgccgtga tggacgtcga
     9301 aatcgaagat gaagactcga tccatgccgg gctgctcgcg gatccattcg gcgacgcagg
     9361 cgatcgtgtt gaagaaacag aatcccatcg cttccagccg ctcggcgtga tgtccgggag
     9421 gccgtgcgac gacgaaagcc cgacccgctt cgctttgcat catctcttcg gccgcggtga
     9481 gcgcggtacc gaccgctgtt cgcgcagcag caaacgtttg cgatgacatc ggattgtcga
     9541 tcgagtggaa gagcctcatg cctgaccgcg atgcctcttc gagcgagcgt tcgtagtcgg
     9601 ctgagtggac cttggcgatg aggcgattcg tgtcgtgatg ctccgccgtc tcgcgtgtaa
     9661 tccgccccgc cagccccgcc cgcgcaacgc cagccagcgc agcatcgagc cgcttcgccg
     9721 tctccggatg cccgacgccg gtgtcgtgtt tatagaagac ggggttcgtg tagatggaaa
     9781 tcatcagagg gtgcgcccag tcgagatcac acacattctg atcgaatgcc acctcggagc
     9841 acagacaaga gtgtctgtgc tacatcgccc tcttcgcgtt tttcgatcat acttccccaa
     9901 tccgtccacc aattgacaag ccgtctctcc gcatcagcag cctcggtgcc gacattcgtg
     9961 ttggcaatga gcgcgaacgc ctcgccccgc gacgcatcgc cgctctgaag agtcatggcg
    10021 gattcgggaa agggccgccg gagctcaaac cgattcccgg ggccagcaac tgggtgcagg
    10081 tcggaccgat ggtcacgacc gacggaatgg cctacgccaa gaacatggtg ttctggacag
    10141 gccggctcac agcgatcgtc gtcgatccaa ccgctcccgc gacgatgtac ctgggcgcgg
    10201 ccaaaggcgg cgtttggaaa accatcaacg gcggcaagag ctggtcgccg atgtccgatg
    10261 acgagctttc cctggccatc ggcgcgatcg cactcgaccc agtcacgccg cagacggtct
    10321 acgcgggcac gggagaaggc aacattttct atgtcgtcaa cgtccctgac tcgacgcagg
    10381 agagctacta cggcgcaggc atcctgaaga gcctgaacgg cggagcgagc tggaacctgt
    10441 atggacaggc ggagttcacc ggcgcagcgt tcttccgcat cgctatccat ccgaccaacc
    10501 cgaaccgcgt gtgggcggcg acgtcgaacg gtctctttct cagcgacagc ggcggcacat
    10561 cgtggacgca cgtcacgaac ggccttcccc cgacggttcc cggcaccgct accggctgca
    10621 cggagatcgt gctccatccg agcgaccctt cgatcgcctt cacctacttc atcggtgagg
    10681 gtgcatacca gtgcacgaac gcaaacaccg ccagtccggg ctttacccct gttccggacc
    10741 tgcaagccgg caacaagttt cgagcgagca tcgcgttctc tccttcccaa ccacagcgca
    10801 tggtggctgg attcgatgac gacctctggt actcgaccga cggcggccag acatggaaac
    10861 cgacgggcgt accgctagtg aaatgcacga tcacctttta ctgcatcaac gtcgcgatcg
    10921 atcccactga ccccaatgtc gtctacctca gcggcgaacc gcagatcatg aaattcacat
    10981 tcgatccggt cggcaaaacg tggagcggca gcaacatcgg gctggggaag acgcacgacg
    11041 atcaccacgc cttagccttc gatccgacga accacctcca catctatgcg ggcaccgacg
    11101 gtgggattta cgaatcgcac gacggcggcg gctcgtggag cgatgacctc aaccgcggcc
    11161 tctccatcac gcaattcgac ttcatcgatc atcaccctgg ttccgatgcg gttgtcttcg
    11221 gcggcacaca ggacaacggc acgcagcagt tccgcaacag caccgtcttc cccgaagtct
    11281 cgagcggcgg cgacggcggt tgcgtggccg tcgatttcaa caatcctcac aacgtcgtca
    11341 tcgagtactt tcacatctca ccggcgcgct cgacggatgc gggcgagtac gcgaccttca
    11401 cggacatctc gcccggcctc ttcggctcaa cgctcttcta tccgccatac gcgctctgtg
    11461 cggaagatcc aacgtggatt gcattcggca gcaccggcat ctttatcgat aaggcgcagg
    11521 ggacgggtgg ctggccgacg ctcgtcaagc taccgggcat taccgggccc atctcggcga
    11581 tcaacttcgt cagcaagatc ctgatttact gcgcgaccat tcacggcgag gtctacgcca
    11641 tccgatcgac gaacggcggc gtcgactgga ctcccacggc catccacgcc gcgccgtttc
    11701 cattgcggtt tgtctgggac atcggcgtca tgccgaacga cagcgcgcac atcatcgcgg
    11761 tcgtctccgg ttacggctcc ggccacgtct ggagcgcgat cgtgccgccg aatggcgtgg
    11821 cggtatggac tgacgtcagc ggcagcggag catccggctt ccccgacgta cctgcgaact
    11881 cgctcgtcat cgatccggcg gcaccgtcga cctactacgt cgggacggac atcggcgtct
    11941 tcaaaaccac gaacgcgggc gggatgtggt cgaacttcgg ccaggggctg ccgaatacgg
    12001 cggtgttcga cttgcgcctg cacggaccat cgcaactgct gcgcgcggct acgcacggcc
    12061 ggggaatctg ggagaggcgg gtggacgtgg cgtcgatgcc tgacgtcgac atctacgtgc
    12121 gccatcacat gatggacaca ggccgcgggc tggcgccgga aggtgttccc gccgcgttca
    12181 agaacgtcgg acagttcgtt gggctcggcg atccgcagcg tcactggatg tgcgtcgata
    12241 tgaaagtcga cgcactgctc ggcaatccgc agtcgtttca gtacgccccc agcgaggtcg
    12301 acttcgtggt ctacgaagcc cagctctacc accgcgacgc cgagcgcgga aacgtaaacc
    12361 gtgtttacgc tcaggtacac aaccgtggga tcaaggcgtc gaatgtgacg gtcaaactgc
    12421 ttttcgccga cgcgacggcg aaggttcctc ccctgccaaa cgatttctgg tccgccttcc
    12481 ccggcaactc cgccgtgccg aactcgccgt ggcacgctat cggcgcggcc aagaccgtct
    12541 ccgtcacgcc cacgctgcca acggtgctgg agtgggactg ggccaccccg atgggtcagg
    12601 ccacgcatac ctgcctgctg ctgatcgtca attcacagga cgacccaatt cccgccgcgc
    12661 acaagatctt cgacgtcgac gcgctggtga agctcgacaa gcgcgcgggg ttgaagaacc
    12721 tgcacgtcgt caacgcaccg cccaaaccgc
//
LOCUS       DATLVW010000454         8846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_102139, whole genome shotgun sequence.
ACCESSION   DATLVW010000454 DATLVW010000000
VERSION     DATLVW010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102139"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VNN25_13835"
     CDS             <1..1410
                     /locus_tag="VNN25_13835"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXH92658.1"
                     /translation="ASGNQWFLNGNPIGGQTNQTYSATASGSYTVTVTTTGCTSSPSA
                     ATVVTVNPNPNATITAPASVVKSSTGNIASVANAGAGATYVWGITNGTITAGTGTASI
                     TFTAGAVGTLTLNVTVTTSANCSDAKSANVNVVLPTVTVTSVSPNSGTVTGGTNVTVN
                     GTNFAAGATVTFGGTAATNVVVVSAIKITAKTPAHAAGAVNVTVTNADTSTATLTSGY
                     TYTAQHFDPNNDGTIDPSDIFFLINYLFMNGPPPHGAGGLLSGDANGDGVVDPADIFY
                     LVNYLFLGGSTPNRPAGSIGTSPITTSAVGAEVPHIAGSIALGKPALRGGHYVVPVVM
                     TMKPGSVVPQAMSLRVHLDSVSTIGAVTVQRAGAAKDVPAVFETTRRSGNDVSYLVAY
                     DPRGLAIGGARSAVIAEIEIDSAEDGVAITVDPILTMLSDLAGTMKATVGNQNLQVSG
                     TTTGSGAVPRPHVPGHEVK"
     gene            1429..2112
                     /locus_tag="VNN25_13840"
     CDS             1429..2112
                     /locus_tag="VNN25_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92659.1"
                     /translation="MKAHAKSLKVICATALMSTLAALLTAGPAPPLPQDVVTVGIVTG
                     PQFTVVDVPVSIRDVSGTPLGLDQPPGSRIQSYSIKVNYAPASAVQSISFSRAGITQS
                     LTPTFENSPSSAGSISLLDTFQESTNLIPFTLNGLPPGNKVAHLLVTLSPTAPVGQVI
                     TLTLDPTLTQLTDEAGDPATIETTANTRLALVSGSITVVPSVPAMSYLVLGLLAVALA
                     FAGLKLIRF"
     gene            2173..4029
                     /locus_tag="VNN25_13845"
     CDS             2173..4029
                     /locus_tag="VNN25_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92660.1"
                     /translation="MNRKPLVGFFALLFSILAFLIVAAPAIAQDAVTVGTATASGNTV
                     DVPVYIRDVAGTTLGRDQAVGSRIQAYSIKVTYAPAAAVQSVTFSRAGITAGLTPASE
                     FSPSTTGSISLLDTFQESTNLIPFTLGAPAPGDQVAHLVFTLSSSAAPGSSISLTLDP
                     SLTQLSNQGGDTKESVSNSGLTLTDGVINIPNLTLALVPGSRSVVLNDTTAIAAQTSA
                     PVQSDTTVSLSSTNPNIATVPASVIIPAGSQFANFSVTAIGIGSVNINAALPASLGGA
                     SASAAVTVTEPPPQCLTPLAPVLSGPSTADAGKAYTISWNSVSNATDYAIDESTDQNF
                     LTISTTNVTAASVSFTHLAPNRYYYRVRARNQSTGCNVSSLFSTAVSILVSVAPVAQT
                     KILPVVGSVPGTGGSYFKTSLQFFNAKTTAISGKIVFHPQAASGSASDPSLAYVIQAG
                     KSLFYPDLLPAMGVASGLGSADVIADAGTAFPVTLVRVFNDGGVAGTTGLALDLMALS
                     DALQNGESGVLIAPADTRFRLNIGVRTLESGASMTLTVRDKDGAVVKTASQSFGPTFF
                     RQLGSSSILDGYTLAGGETITVQITSGSAFVYGSTTDNTTQDPSVQFAKRIE"
     gene            4320..7679
                     /locus_tag="VNN25_13850"
     CDS             4320..7679
                     /locus_tag="VNN25_13850"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH92661.1"
                     /translation="MGVRPPSQDEIGTPAGAIPVGPPIESAMTKAGSAQQLDLRFLPQ
                     VFVQEHVERPERQLPPVNPTQIPGDLVPAPPTLPAFPTVNAPAPSPSASFAGLDSSWS
                     DGLPPDANGDVGPAYYIQTINTAVGVYRKSDGLRMAAFSFNSLMSQGHFGNLCDTNNY
                     GDPVVLYDSFEDRWIITDFAFKLSGYDIINPPGSFQCFAVSITGDPLSGGWHFYSINT
                     AGGMGDYPKFGIWPDGLYMTANMFDYLYFGPYLGPRVYAFNKAQMYAGKSSIQVVSFN
                     GPSYEATILPANARLQAGTPPAGSPNYYADYNYTNAVSIYKFHVDWSRTSLSSFTGPF
                     NSTAPNSWAYAPSSVPALNGVGLDSLNDRLMAQNQYTNQGGVESLWMNHTVLGAAVST
                     AAPRYYQVGVTGGTVAGNTSQAFTYTPDSTMNRFMGSLAVDRAGDMALGYSTTSSSSY
                     PAIKYAGRLATDPVNSLPQTEVPLVSGTGSQSGINRWGDYSAMTLDPDGCRFWYTNEY
                     YATTSGYSFLTRIGAFAFPSCTPVGSGGTLQGTVTAVSGGAPVSGVAVAFGARSAATN
                     ASGLYSFAGIPAGTYPAVSVSKPGYVSQTAAPIVITDGGSTTKDFALSGAASSGCLTD
                     TTQSDFQTGVATTVDLNASPGNVTLATALTGNLVSGLKDSNPAAGSLVVWSALSWTAT
                     TPANTSVKFQVAGSNSDSGPFSFVGPDGTNATFFTTTGSSLSQFSGKRYLEYKAYLAT
                     TTNTVKPTLSDVTLCYSNADCSGASATITTSPHVCSSSPGNTASGPAGKASYLWSITS
                     TGTITSGATSQTMTFSSGTDPQINLSLTTVDGAGCQASAVAGVYVITGPTVNPASISN
                     GTVGAAIYVPFSASGGAPPSTFSLTGSLPPNVLFSSGTLSGTPNTTGTYTFTVTATDT
                     NGCSGSRTYTLTIATPAGTAPANMTATAINTTQVFLSWTPVGSATSYDVIRTAAGGGP
                     VVIGSTSSTVYGDTQGLVAGSTYIYVVRANGPGFPVNAISLPDIATMVVFTDDPLIVG
                     STSFKGVHLTELRSAVNAVRAVAGLTAATFTDAAPAGKTAKALYIQELRAALDPARSA
                     IGVPAMIYSNAASAGTLVRAIDLAEIRNGVK"
     gene            complement(8062..8292)
                     /locus_tag="VNN25_13855"
     CDS             complement(8062..8292)
                     /locus_tag="VNN25_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92662.1"
                     /translation="MARTTLEIEDDALKVARAYAARHRISLGKAVSELLRQSVDRPLT
                     TDVRNGLHVVRFSRRSPKVTTAIVNKLRDEFL"
     gene            complement(8340..8765)
                     /locus_tag="VNN25_13860"
     CDS             complement(8340..8765)
                     /locus_tag="VNN25_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXH92663.1"
                     /translation="MSMSAGSSHGVRSEINITPLVDVVLVLLIIFMVATPILQMGLDV
                     EVPPKVEVTGAPPPSEQNQLVVTVRADGIYLNSLKLADAKALRDALAPQMLSRPVADR
                     VVFVNAEDAVLFDRAIQAMDAAHEAGAKKVGFLTDVPGK"
BASE COUNT         1724 a         2891 c         2555 g         1676 t
ORIGIN      
        1 gccagcggca accagtggtt cctcaatggc aacccgatcg gcgggcagac caaccaaact
       61 tatagtgcaa ccgccagcgg cagctacacc gtcacggtca cgacgacggg ctgtacgagt
      121 agcccgtcag cggcgacggt cgttaccgtc aatcccaatc cgaacgccac catcactgca
      181 ccggccagcg tcgtgaagag ctcaaccggc aacattgcct cggtggccaa cgcaggcgcc
      241 ggcgcaacct acgtctgggg catcacgaac ggcacgatca cagccggcac gggtaccgcc
      301 agcatcacgt tcacagcggg cgcagtcgga acgctgacgc tgaacgtgac cgtgaccacg
      361 agcgccaact gcagcgacgc gaaatcagcg aacgtgaacg tcgtcctccc gacggtcacc
      421 gtgacttcgg tctcgccgaa ctccggcacc gtcaccggcg gcacgaacgt caccgtcaac
      481 ggcaccaact tcgccgccgg cgcgacggtc accttcggcg gcacggccgc gacgaacgtc
      541 gtggtggtca gcgcgatcaa gatcaccgca aagactccgg cgcatgcagc aggagcggtc
      601 aacgtcaccg tgacgaatgc cgatacatca accgccacac tcacgtcggg ctatacctat
      661 acggcgcaac acttcgatcc gaataacgac ggcacgatcg acccgtccga catcttcttc
      721 ctcatcaact atctattcat gaacggaccg ccgccacacg gcgccggcgg tttgttgtcc
      781 ggtgacgcga acggtgacgg tgttgtcgat ccagccgaca tcttctatct cgtcaactac
      841 ctcttcctcg gtggctcgac acccaatcgc ccggccggca gtatcggtac gagcccgatc
      901 acaaccagcg ctgtaggcgc agaagtgccg cacatcgcgg gttcgatcgc cctcgggaag
      961 ccggctcttc gcggaggcca ctacgttgtg ccggtcgtca tgaccatgaa acccggttcg
     1021 gtcgtgccgc aggcgatgtc gctgcgcgtg cacctcgata gcgtttccac gatcggcgca
     1081 gtgaccgtgc aacgtgccgg cgcagcgaag gatgttccag ccgtctttga gaccacacgc
     1141 cgctcgggta acgatgtgtc gtatctcgtg gcgtacgatc cgcgcgggct cgcaatcggc
     1201 ggggctcggt cagcggtcat cgccgagatc gagatcgact ctgctgaaga cggtgtcgca
     1261 atcacggtgg atccgatact gacaatgctc agtgatcttg ccggaacgat gaaggccacc
     1321 gtcggtaatc aaaatctgca agtgagcggc acgacgacgg gcagcggcgc agtgccgcgg
     1381 ccgcatgtgc cgggacacga agtgaaatag ggtggggaga gaaaacgcat gaaagcgcac
     1441 gcaaaatcat tgaaggtcat ttgcgccacg gctctgatga gcactctggc ggctcttctc
     1501 accgcaggac cggccccccc attgccgcag gacgtggtta cggttggaat cgtgactggc
     1561 ccgcaattca ccgtggtcga tgtgccggtt tccatccgcg acgtgtcggg cacccctctg
     1621 ggactcgatc agccgccggg ctcgcgcatc cagtcgtaca gcatcaaagt gaactacgcg
     1681 ccggcgtcgg cagtgcagtc gatctcgttt tcgcgcgccg gcatcacgca gtcgttgacg
     1741 cccacgttcg agaacagtcc ctccagcgcc ggatcgatct cgctgctcga cacctttcag
     1801 gagtcgacga acctgatccc cttcaccctg aacggcctcc cgccgggcaa caaggtcgca
     1861 catcttctgg tcacgctgtc gccgacggcc ccggtcggcc aggtcatcac gctgacgctc
     1921 gatccgacgc tcacgcagct caccgatgaa gcgggagatc cggcaacgat cgagaccacc
     1981 gctaatacca ggttggctct cgtcagcggt tccatcacgg tcgtacccag cgttccggcg
     2041 atgtcttatc tcgtcctcgg actactggcc gttgcgctcg cattcgccgg cctcaagctc
     2101 attcgcttct aaagtcaata gtctagtcat cacgcgaggc aaacaatctt cgatccccta
     2161 aggccccggc aaatgaatcg caagcccctc gtcggttttt tcgcgttgct cttttcaatt
     2221 ctcgctttcc tcatcgtcgc cgcgccggcg atcgcccagg atgcggtgac ggtcggtacc
     2281 gccaccgcat ccggtaacac cgtcgatgtt ccggtttaca ttcgcgatgt cgccggcaca
     2341 acactgggcc gcgaccaggc ggttgggtcg cggattcagg cgtacagcat caaggtcacc
     2401 tatgcacctg ccgcggcagt ccagtcggta acgttctcgc gggccggcat cactgccggt
     2461 ctgacccccg cctcggagtt ttcgccctcg actacgggtt cgatctcgct cctcgacacg
     2521 tttcaggaat cgacgaatct cattcccttc acgctcggcg caccagctcc cggcgatcag
     2581 gttgcgcatc tcgttttcac actgtcctca tcggcagccc cgggaagctc catctccctc
     2641 acgctcgatc catcgctcac gcagctcagc aatcaaggcg gtgacaccaa ggaatcggtg
     2701 tcgaacagcg ggctcaccct tacggacggc gtcatcaata tcccgaatct cacgctggct
     2761 ctcgttcccg gctcgcgctc cgtcgtactc aacgacacca ccgcaattgc ggcacagacg
     2821 agcgctcccg tacagagcga cacgaccgtt tcgctatcgt cgacgaatcc gaacatcgcc
     2881 acggtcccgg cctcggtgat cattccggcc ggatctcagt ttgcgaattt cagcgtgacc
     2941 gccatcggta tcggcagtgt caacatcaat gcagcgcttc cggcgtcgct cggcggagcc
     3001 agcgcctccg ccgccgtgac ggtgaccgaa ccaccgccgc aatgcctcac gccactggct
     3061 ccggtcctct cgggaccgtc gacggcggac gccgggaagg cctacacgat cagctggaac
     3121 agtgtcagca acgccacgga ctacgcgatc gatgagtcga cggaccagaa tttcctgacc
     3181 atttcaacga cgaacgtcac ggccgcatcg gtctcgttca cgcatttggc tcccaatcgc
     3241 tactactacc gtgtccgcgc ccggaatcag agtaccggct gcaacgtctc gtccttgttc
     3301 tcgacggcag tctcaatcct ggtaagcgtg gccccggtcg cgcagacgaa gatcctgccg
     3361 gtggtcgggt cggtgcccgg aacgggtggc tcgtacttca agacgtcgct ccagttcttc
     3421 aacgcaaaga ccactgccat ttccgggaag attgtgtttc atccccaagc tgcttccgga
     3481 agcgcttccg atccttccct cgcctatgtc atccaagccg ggaagagcct tttctatccc
     3541 gatcttcttc ctgcgatggg tgttgccagc ggtcttggca gcgcggatgt cattgccgac
     3601 gctggtaccg cctttcctgt cactctcgtc cgcgtgttca acgacggcgg tgtagcggga
     3661 acgacagggt tggctctgga cctgatggct ctgagcgatg cgctccagaa cggcgagagc
     3721 ggagtgttga tcgcgcctgc cgacacgagg ttccgcctca acatcggcgt gcgtacgctg
     3781 gaatcgggag catcgatgac gcttacggtt cgcgacaaag acggcgcagt cgtgaagaca
     3841 gcatcgcaga gctttggtcc gacgttcttc cggcagctcg gatcgtcatc gattctcgac
     3901 ggatacactc tcgccggcgg ggagacgatc acagttcaga tcacctccgg cagtgcgttc
     3961 gtgtatggct cgacgaccga caacaccacg caagatccga gcgtgcagtt cgcgaagagg
     4021 atcgagtaac acgcgtcaca ggcgccctta aacacagatg gtagagtctc gcgacacttc
     4081 gccggcgcgg gttcgtccac cgcgggctgt cggttcgaaa aaaccatgtg cgtccgacct
     4141 ccagtgagca cccgccccag atgaaacgcc ttcttcgatt cgcgctgctg gcgttgacga
     4201 tttcatctcc cctcagcctc tccgcggcgc ggaaaacggt tgttgaggaa ccgttggaca
     4261 ccgcagttcc aaccccagcg ctcattcgtt cgccgcgccg aaccccgcag aggaaccgaa
     4321 tgggcgtgcg gcccccgtct caggacgaga tcgggacgcc ggccggcgcg attcctgtcg
     4381 gtcctccaat cgagagcgcg atgaccaagg ccggcagcgc gcagcaactc gatctccggt
     4441 ttcttccgca ggtgttcgtg caagagcacg tggaacggcc agagcgtcag cttccacccg
     4501 ttaatcccac gcagattccc ggagacctgg tgccggcgcc gccaacactt ccggcgttcc
     4561 cgacagtgaa tgctccagcg ccttcgcctt cggcaagttt cgccggtctc gactcgtcct
     4621 ggagtgacgg tcttccgcct gatgcgaacg gcgacgtcgg gccggcttac tacatccaga
     4681 cgatcaacac cgccgtgggc gtttaccgga aatcggacgg cttacgcatg gctgcgttct
     4741 ctttcaactc gctcatgagc caggggcact tcgggaacct ctgcgacacc aacaactacg
     4801 gcgatcctgt cgtactttac gactcgttcg aagaccgctg gatcatcacc gactttgcgt
     4861 tcaagctcag cggctatgac atcatcaacc ctcccggttc cttccagtgc tttgccgtat
     4921 cgattaccgg cgatccgctc agcggcggct ggcacttcta ctcgatcaac accgccggcg
     4981 ggatgggcga ttatccgaag ttcggtatct ggcccgatgg cctctacatg acggccaata
     5041 tgttcgatta cctctatttt ggtccctacc tgggcccgag ggtgtacgcg ttcaacaaag
     5101 cgcagatgta cgccggcaaa tcctcgattc aggtcgtctc attcaatggg ccgtcgtacg
     5161 aagccacgat ccttccggcc aatgcgcggc tgcaggctgg cacgcctcca gcgggctctc
     5221 cgaactatta cgccgactat aactacacga acgcggtctc gatctataag ttccatgtgg
     5281 actggagcag gacttctctc tcctcgttca ccggtccgtt taattcgacg gcgccaaaca
     5341 gttgggccta tgcgccaagc agcgtaccgg cgctgaatgg cgtggggctg gactccttaa
     5401 atgaccggct gatggcgcag aaccagtaca cgaaccaggg cggtgtcgaa tcgctctgga
     5461 tgaaccacac cgtcctcggc gccgctgttt caacagcggc tccgcggtac taccaggtcg
     5521 gtgtgaccgg tggaacggtt gcagggaata cgagccaggc cttcacctac acgccggaca
     5581 gcacgatgaa ccgtttcatg ggcagcctcg ccgtcgatcg ggcgggcgac atggccctcg
     5641 gatacagcac aacgagctcg agctcgtatc cggccatcaa gtatgcaggc cgcctcgcaa
     5701 cagacccggt caacagcctt cctcagaccg aggtaccgct ggtcagcgga acgggatcgc
     5761 agagcgggat caaccgatgg ggcgactaca gcgccatgac gctcgatccc gatggctgcc
     5821 ggttctggta caccaacgag tactacgcaa caacctcggg ctacagtttc ctgacccgaa
     5881 tcggagcatt cgcgttccct tcatgtacgc cggtcggaag cggcggaacg ttgcagggaa
     5941 ccgtcaccgc ggtttccggc ggcgcgccgg tcagcggggt ggccgtcgcc ttcggcgcgc
     6001 gatcggcggc taccaatgcc tccggtctct actctttcgc aggcatcccg gcgggaacct
     6061 atcccgcggt cagcgtcagc aaaccgggat atgtctcgca gactgcggca ccgatcgtaa
     6121 tcacagacgg cggctcgaca accaaagact tcgcgctcag cggcgctgca tcgagcggat
     6181 gtctgaccga cacgacgcag tcggatttcc agaccggggt ggcgaccacg gtcgacctca
     6241 acgcgagccc cggcaacgtc acgctcgcga ctgctttgac cggcaatctc gtctcgggat
     6301 tgaaagacag caatcccgcg gcagggtcgc tggtggtctg gtcggcactt tcgtggacgg
     6361 cgacgacgcc ggccaacacc tcggtgaagt tccaggttgc cggcagcaac tccgactcgg
     6421 gtccgttcag ttttgtcggt cccgacggga cgaacgcgac attcttcacg accaccggat
     6481 cgtcgctcag tcagttctcc ggaaagcgtt atctcgaata caaagcgtac ctggccacca
     6541 cgaccaacac ggtaaagccc acgctgagcg atgtcacgct ctgttactcg aacgccgact
     6601 gcagcggtgc cagcgcgacg attacgacct cgccacatgt ctgctccagt tccccaggca
     6661 atacggctag cggaccggcc gggaaagcga gctacctgtg gtcgatcacc agcaccggca
     6721 caatcaccag tggcgcaacg tcacagacga tgacgttttc gtcgggaaca gaccctcaaa
     6781 tcaacctgag cctcacgacc gttgacggcg ccggatgtca ggcctcagca gtggcgggcg
     6841 tttacgtcat caccggtccg acggtcaatc cggcctcgat cagcaacggt accgtcggtg
     6901 cggcaatata tgtgcccttt agtgccagcg gaggggcacc accgagcacg ttcagcttga
     6961 cgggatcgct gccgccgaac gttctgttct cgagtggaac gttgagcgga accccgaaca
     7021 ccactggtac ctacacgttc acggtcacag cgacggatac aaatggttgc tcgggctcgc
     7081 ggacgtacac attgaccatc gcaacgcctg ccggaactgc gcctgcaaac atgacggcga
     7141 cggcaatcaa cacgacgcag gtgtttcttt cctggacccc ggtcggatcc gcaacctctt
     7201 acgatgtcat tcgaaccgcg gccggcggcg ggccagtcgt catcggatcg acatcgtcca
     7261 ccgtttacgg agatacacag ggcctcgttg ccggatcgac ttacatttac gtcgtgcggg
     7321 ccaacggacc aggctttccg gtcaacgcga tcagcctgcc cgacatcgcc acgatggtcg
     7381 tgttcacgga cgatccactc atcgtcggat cgacctcgtt caagggcgtt cacctcaccg
     7441 agttgcggag tgcggtcaat gcggttcgtg ccgtggccgg actcacggca gcaacgttca
     7501 ccgacgctgc tcctgccggc aaaaccgcga aggcgttgta catccaggaa ctgcgtgccg
     7561 cgctcgaccc cgcgcgctca gcgatcggcg ttcctgcgat gatctacagc aatgccgcga
     7621 gcgcgggcac gctcgttcgc gcgatcgatc tggccgagat ccgcaatggg gtgaagtgag
     7681 agcatttcac aggcggacgg aacccaattg cgtgaccggg aaggttgccc attgtgcctc
     7741 taacggattc cgaaccactg ccgcgcggcc tcatcgaaca aagccctatc gcgactctct
     7801 ccaatcgagc gccttctccc cccgtgcttt tcggggggag aaggtgccga aggcggatga
     7861 ggggggcgca agattggaga aacgtgttgt tcgcgagcgt gaaccgattt ggacctgcct
     7921 ggcgcctttg caacgttgca gcgacagcca aacgccccct catccggcct tcggcgctcg
     7981 attgaaagcg tggtgagaca atttcaggag gagtgtgaga aatgcgggct agaagtatgt
     8041 tgaggtcgaa gacagcggac ttcacagaaa ctcgtcgcgc aacttgttga cgatcgccgt
     8101 cgtcaccttg ggtgagcgcc ggctgaaccg cacgacatgc aagccatttc gaacatcagt
     8161 agtgagcggg cggtccaccg attgccgcag cagttcgctg acggctttgc ccagactaat
     8221 gcgatgacgg gcggcgtacg ctcgcgccac cttcaacgca tcatcttcga tctcaagtgt
     8281 ggttcgtgcc attaccgtat caaacccgaa aaccgatcgt gtgttgctct acgcggcgtt
     8341 cacttccccg gcacgtcggt caggaagccc accttcttcg cgcctgcctc gtgcgcggca
     8401 tccatcgctt gaatagcccg gtcgaacagc accgcatcct cggcgttgac gaagaccacg
     8461 cggtctgcga ccggacgcga aagcatctgc ggcgccagtg cgtcgcgcag cgcttttgcg
     8521 tcggcgagct tgagcgagtt gaggtagatg ccgtccgcgc gcaccgtgac gacgagctga
     8581 ttctgctccg agggtggggg ggcgccggtc acttcgacct tcggaggaac ctccacatcg
     8641 agtcccatct gcaggatcgg tgtggccacc atgaagatga tgagaagtac gagcaccacg
     8701 tcgaccagcg gcgtgatgtt gatttccgat cgaaccccgt ggctcgagcc agcggacatt
     8761 gacattggtt ttctcctggg gtgtggggag tagggagtgg ggtgcgggag aaaggcgagg
     8821 gctccgtaag cgctctcagt tcttcc
//
LOCUS       DATLVW010000455         2697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_516362, whole genome shotgun sequence.
ACCESSION   DATLVW010000455 DATLVW010000000
VERSION     DATLVW010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516362"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..922)
                     /locus_tag="VNN25_13865"
     CDS             complement(251..922)
                     /locus_tag="VNN25_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92664.1"
                     /translation="MPQQKDFKRVVRTRMQKTGESYTTARLHLVKKQSPAEPVAPPED
                     YAALAKLSDESVKKATGCDWSKWVFVLDKAGGKEMSHSELVAHIREKYKTPGWWTQMV
                     AVGYERIRGLRAQGQMRSGDWSVTKSKTINVPLAKLYAAFSNARTRAKWLPGVKVTVR
                     SATPGKYIRLRWEDGAPIAVGFYAKGESKSQVALEHSKLATKADADRIRAFWSERLNA
                     LAAIF"
     gene            complement(1164..2420)
                     /locus_tag="VNN25_13870"
     CDS             complement(1164..2420)
                     /locus_tag="VNN25_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH92665.1"
                     /translation="MFLLALIAVAAIGCAAAFATLWSRTNNDIRRLSDRLGRPDGEAS
                     ASLDVDSFGALDEIASRIYRSRADLARQLSAAEFQNALLQQIMNGMGEGVLAIDRQRR
                     VVLANRRFAAMFNLDAGFVGQALSAVLRNEAILSGFDRALDRTSGSTDSASRFSLRSG
                     IEERTIEMRAFPLPSHDLAAVALFIDVTRVERLEELRREFIADFSHEVRTPLAALKSA
                     VETFEYDRGHLTEEQDGQLRRIMTRQLARLERLAQDLSELSHIEAGDLSLERAEIDLR
                     RLVEDLCEDFRDRAAQKELRFVVSGGGARVHGDAMRIQQAISNLLDNAIKYGGEGTSI
                     DIEVTAGPDSGIVRITDHGEGIPPEERDRIFHRFYRIDKSRSQEIAGTGLGLAITKHL
                     ILQHRGNIEVESEPGKGATFVVTLPK"
     gene            complement(2508..>2697)
                     /locus_tag="VNN25_13875"
     CDS             complement(2508..>2697)
                     /locus_tag="VNN25_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH92666.1"
                     /translation="LLLIRNRGSVISRDRILSEVWQMSDDVETRTVDAHIRNLRKKIG
                     TERITTVVGFGYRYDPPV"
BASE COUNT          472 a          812 c          881 g          532 t
ORIGIN      
        1 acgggaatct ggctccggcg ccggctacgg gggtggagat tcgggagtga ctgttcgggg
       61 gtcgggggtc gggggtcggg ggtcggaagg gtgaacgccg ccttccccat tccatgtttt
      121 tcaacgacct ttgcaaacgt caagactagt caatcgagcg ccttctcccc ctgcgctttt
      181 tgcaggggga gaaggtgccg aaggcggatg agggggcaca actacgctgc accaccagcc
      241 caaggggaaa ctagaatatc gccgctaatg cgttcaaccg ttcactccaa aaggcgcgga
      301 ttcggtcggc gtcggctttc gtggcgagtt tgctgtgctc gagagcgact tggcttttcg
      361 attcaccttt tgcgtagaag ccgacggcga tcggggcgcc gtcttcccag cgaaggcgga
      421 tgtatttgcc cggcgtagcg ctgcgaacgg tcacctttac tccgggcagc cacttcgcgc
      481 gggttcgggc gttgctgaag gcggcgtaga gcttcgccag cggaacgttg atcgtcttgc
      541 ttttggtgac cgaccagtcg ccgctacgca tctggccttg cgcgcgcagt ccgcggatgc
      601 gctcgtagcc gacggcgacc atctgcgtcc accagcccgg cgtcttgtac ttctcgcgga
      661 tgtgcgcgac cagctccgaa tgcgacatct ctttgccgcc ggctttgtcg agcacgaaga
      721 cccacttcga ccaatcgcag ccggtggctt tcttcaccga ttcatcgctg agcttcgcca
      781 gcgcagcgta atcttcggga ggagcgacag gctccgcggg tgattgtttc ttgacgagat
      841 gaaggcgggc ggtggtgtac gactcgccgg tcttctgcat gcgcgtacga acgacgcgct
      901 tgaagtcctt ctgctgtggc atcgcgaaat tctccttcgt tcacgttcgc gagcaccgct
      961 cccagcagcg aagccctcga acgaaggttc gcaagtaccc gacccgggag tatgcccccc
     1021 tttgcctccg cgtcgggcct tcgctgggga aggtctcagg aggttcggcg cgagtcaatc
     1081 gccggggtaa ttgtatccgc agatgacgca gatgtcccgc cgatggttcg aaaaatctgc
     1141 gctcatctgc gacatctgcg gactcacttc ggcaacgtca ccacaaacgt agcccccttc
     1201 cccggctcgc tttccacttc gatgtttccg cggtgctgga ggatgaggtg tttggtgatg
     1261 gccaggccga ggccggtgcc ggcgatctcc tgggaacggc ttttgtcgat gcggtagaag
     1321 cggtggaaga tgcggtcgcg ctcctcgggc gggattcctt cgccgtgatc ggtgatgcgg
     1381 acgatgccgg agtcgggtcc ggccgtcact tcgatgtcga ttgacgttcc ttccccgccg
     1441 tacttgatgg cgttgtcgag aaggttggag atggcctgct ggatacgcat ggcgtcgccg
     1501 tgaacgcggg cgccgccgcc gctgacgacg aagcgcaact ccttctgcgc ggcgcggtcg
     1561 cgaaagtctt cgcaaagatc ttcgaccagt cgccgcaggt cgatctcggc gcgctccaga
     1621 ctgagatcgc cggcctcgat gtggctcagt tccgaaaggt cctgcgccag acgctcgagc
     1681 cgcgccaact gccgcgtcat gatgcggcgg agctggccat cctgctcctc ggtcagatga
     1741 ccgcggtcgt actcgaatgt ctcgacggca gacttgaggg cagcgagcgg cgttctcact
     1801 tcgtgcgaga agtcggcgat gaattcgcgg cgcagttcct cgagcctctc gacgcgcgtg
     1861 acgtcgatga aaagggcgac ggcagcgaga tcgtgcgacg gcagtgggaa ggcgcgcatc
     1921 tcgatcgtcc gctcctcgat gccgctgcgg agcgagaaac ggctggcgga atcggtggaa
     1981 ccggacgtgc ggtcgagcgc gcggtcgaaa ccggaaagga tggcctcgtt gcgaagaaca
     2041 gcggacagcg cctgtccgac gaatcccgca tcgaggttga acatcgccgc gaaccggcga
     2101 tttgcgagga cgacgcggcg ctgccggtcg atggccagca cgccctcgcc catgccgttc
     2161 atgatctgct ggagcagcgc gttctggaac tccgccgcgg acagttggcg ggcgagatcc
     2221 gcacgcgaac ggtaaatgcg ggaggcgatc tcatcgagcg caccgaagga gtcgacgtcg
     2281 agggacgcgg atgcttcgcc gtccgggcgc ccgaggcgat cggagaggcg gcgaatgtcg
     2341 ttgttggttc gcgaccacaa cgtagcgaat gcggcagcgc atccgattgc ggcaacggcg
     2401 atgagggcga ggagaaacat attgctcatc cgcagatgta cgcagatgta cgcagatctt
     2461 ccttcatctg tgctcatctg cgtaatctgc ggatcgtaat ctgcaaatca aaccggcgga
     2521 tcgtaccgat agccaaaccc caccaccgtc gtgatccgct ccgttccgat cttcttccgc
     2581 agattgcgga tgtgcgcgtc aaccgtcctc gtctccacgt catcgctcat ctgccagacc
     2641 tcggacagga tccgatcgcg cgagatcacg gagccgcggt tgcggatgag gagcagc
//
LOCUS       DATLVW010000456         5559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_228711, whole genome shotgun sequence.
ACCESSION   DATLVW010000456 DATLVW010000000
VERSION     DATLVW010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228711"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VNN25_13880"
     CDS             <1..1331
                     /locus_tag="VNN25_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXH92667.1"
                     /translation="TSIASDLPARGMTYALVEDRVDPKLLYAGTEYGLFVSQNGGSSW
                     FQLKGNFPTVAVRDLWFQKRHDDLVIATFGRGFWVLDDVSPLRAMTPAIAANEATLFP
                     TRDADLFVERAQYGLPGKSFQGATFFTAPNPPFGAVFTYYLKDELKSRRKQRQEAESK
                     IDQDKSNEPGHAPPPYPTLDQLRAEDREIEPAIVMIVSDEEGNVIRRVSGPAKAGFHR
                     VAWDLRYPAPNPIELKEPEADVFSPPPGGPLVAPGKYTVRVAKRIDGVETLLGSPQTF
                     NVVPLYLSIMKESDRAAVLDFQKKAAALQRTLMGAARVTQESLTRVQYIRRALDEIAG
                     PDPKLLAKVNTIDASLRDINDQLNGDPILRRANEPFAPSLLDRVNTAVNGLTTTAPPT
                     ATHREALAIAQQQTTPLLDRLHKVIEVDLADVEKQMNARGAPWTPGRIPQ"
     gene            complement(1372..2037)
                     /locus_tag="VNN25_13885"
     CDS             complement(1372..2037)
                     /locus_tag="VNN25_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92668.1"
                     /translation="MIAEPMTAFTDLLLCLLSLGFGIRLRGGTSRARSLWSLAFLASS
                     LGSLTGGTYHAFQPSLSPLLLTILWKITAAALAAAGLLLFCGTTQALVPQRMARLLTI
                     IASLLFVIYLAWMTTHDEFIYIIVFYGTMMIGTLATCIVRYRIAPRPCALIISGIAIA
                     ALAAAVQASHIDPFVHFNHNDVNHVIQMISLYVMYRGARQLAREENLTTETQRAQRET
                     PTL"
     gene            complement(2135..3517)
                     /locus_tag="VNN25_13890"
     CDS             complement(2135..3517)
                     /locus_tag="VNN25_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXH92669.1"
                     /translation="MTSVLLNDIHSQLNETRVDRVISVASQEAVQNAVLDAARYEKSI
                     SISGGRHSMGGQQFGAGTVHLDMRALNRIVTLDAARGVAEVEAGIQWPELMRGLELMQ
                     EGSESSWCIVQKQTGADRLTIGGALASNVHGRGLIWPPIINDVEAFTLIDHAGEARRC
                     SREENRKLFSLAIGGYGLFGVITSVALRLRPRSRVERVVEIRNIEGIIEAIQQRIADG
                     YLYGDFQFSIDEKSPGFMREGVFACYRPTDLPLGTARELGVGDWEELIALAHFDRRRV
                     YEVYSTYYQSTSGQIYPSDTHQSGLYIDDYHHAVDAQTGSRATEMITEVYVPRQRLID
                     FMDEVRADFLAHHTPNIYGTIRMIEKDEESFLVWAKEAYACVIFNLHVEHTIDGLQSS
                     ADAFRRLIDMAIARGGSYYLTYHRFARRDQVESCYPQFAQFLAMKRRYDPNERFQSDW
                     YRHYRDMFGA"
     gene            complement(3567..>5559)
                     /locus_tag="VNN25_13895"
     CDS             complement(3567..>5559)
                     /locus_tag="VNN25_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH92670.1"
                     /translation="MYVVCVQVSLMTRISTFFLGCSLFATTMFADSAKVDMQAYAANP
                     LQVSMAVVNHGPDVARNSFLTIDFPPELVLDRVVSVYGPCDYTQRPVRCSLGDIPVFA
                     PETNTFAYYLEANFKGPFVDASYKVTFTISSDTPDPTPSTNSASVMLTTKLEADLGVL
                     IDRSPRFERIDPGQNAEFWTYVGNNVRGNKPPNVRIDYAVTNGVIEKIDAPSPVSCSI
                     AGATAVCTISPLVENPNTITVTVKTTADRLAGKATLSMRAAGDVPDKTPANDQTENTV
                     PIYRWYAVTTVADDGPGSLRDAIVQANANCTPGPCRIVFEIPPPVPAEGWFTITPAEP
                     LPTITADRVSVEGLRQKAFTGDTNPLGPEIAIDGRLARRGLRIVARCEAVIEGLALGN
                     FDEDQGLWFGGGAGCPEAFRPDRRAVASNYIGVDPTGTTAWPNLRGLNAGGANVLVSN
                     NVISHNRNSGVWMWNGSAQFETNRIESNGASGIFLGPGVDDITITGNTISNHRDMGVA
                     VARSKAHVEIHSNTMKNNGGLGIDWGLDGVSPVDGNDKDGPSNAPVLLSATYDAAQNR
                     TFITLSIKSTPLSTTTQQDGVIDFYGNGAPDGEGEKTLGSVHASSATTGTITVSVGGD
                     LRGQWINATWTRIIPNASDPYYHDAAVTSELSNAIFASK"
BASE COUNT         1066 a         1662 c         1771 g         1060 t
ORIGIN      
        1 ggacatcgat cgcgagcgat ctccccgcgc gcggcatgac gtacgcgctg gttgaggacc
       61 gcgtcgatcc aaagcttctc tacgcgggaa cggagtacgg actcttcgtc tcgcagaacg
      121 gcgggtcatc gtggttccag ttgaaaggca atttcccgac cgtggccgtg cgcgacctct
      181 ggttccagaa gcggcacgac gatctcgtca tcgctacgtt cggccgcggc ttctgggtgc
      241 tcgatgacgt cagcccgctg cgcgcgatga cgcccgcgat cgccgcgaac gaagcgacgc
      301 tctttccgac acgcgacgcc gatctcttcg tcgagcgcgc ccagtacggt ctgccgggca
      361 agtcgttcca gggcgcaacg ttcttcaccg ctccgaaccc gccgttcggc gccgtcttca
      421 cctactacct gaaggacgag ctgaagagcc gccgcaagca acggcaggag gccgagtcga
      481 agatcgacca ggataagagt aatgaaccgg gccacgcacc gccgccgtac ccgacgctcg
      541 atcaattgcg cgccgaagat cgggagatcg agccggcgat cgtgatgatc gtgagcgatg
      601 aagaggggaa cgtgattcgc cgcgtgagcg gtccggccaa agcaggcttc catcgcgtgg
      661 cctgggatct tcgttacccg gcgccgaacc cgatcgaatt gaaggaaccc gaggccgatg
      721 tcttctcgcc gccgccgggc ggcccgctgg tcgcgccggg caagtacacg gttcgcgtcg
      781 ccaagcgcat cgacggcgtc gagaccctcc ttggatcgcc gcagaccttc aacgttgtcc
      841 cgctttatct atcgatcatg aaggagagcg accgcgccgc cgtgctcgac tttcagaaga
      901 aggccgcagc tctgcagcgc acgctgatgg gtgccgcacg cgtcacgcag gagtcgctga
      961 cacgagtcca gtacatccgc cgggcgttgg acgaaatagc tggccccgat ccgaaactcc
     1021 tggcgaaggt gaacacgatc gacgcgtcat tgcgcgacat caacgaccag ctcaacggcg
     1081 atccgatcct gcgccgcgcg aacgagccct tcgccccgtc gctgctcgac cgagtcaaca
     1141 ccgccgtgaa cggcctgacg acgaccgcgc cgccaactgc cacgcaccgc gaagcgctcg
     1201 ccatcgccca gcagcagacc acccctctcc tcgaccgcct gcacaaagtg atcgaagtcg
     1261 acctggccga tgtggagaag caaatgaacg cccgcggcgc gccgtggacg ccggggagga
     1321 tcccgcagtg atgttcgtgt gaaagtctga atcaccacag agacacagag gtcacaaagt
     1381 tggggtttcc ctttgtgctc tctgtgtctc tgtggtgaga ttttcttctc gagccaattg
     1441 ccgagcccct cgatacatca cgtacaacga gatcatctga atgacgtggt tgacatcatt
     1501 gtgattgaag tgaacgaagg gatcgatgtg ggaagcctgc actgcggcag ccagcgctgc
     1561 gatggcgatt cctgaaatga tcagcgcgca cggccgtggc gcgattcgat agcgcacgat
     1621 gcaagtcgcc agcgtcccaa tcatcatcgt tccgtagaac acgatgatgt agatgaactc
     1681 gtcgtgcgtg gtcatccagg cgaggtagat cacgaagagc aagctggcga tgatggtcag
     1741 caggcgcgcc atccgctgcg ggacgagggc ttgcgtcgtt ccgcagaaga ggagcaggcc
     1801 ggcggcagcg agtgcggcgg cggtgatctt ccagagaatc gtgagcagaa gcggcgatag
     1861 cgacggctgg aaggcgtggt aggtgccgcc tgtgagggag ccgaggctgg aggcgaggaa
     1921 agcgaggctc cagagcgagc gggcgcggct cgtgccgccg cgcaggcgga tcccgaatcc
     1981 caggctgaga aggcagagga ggagatcggt gaacgcggtc atcggttcgg cgatcatcgg
     2041 tttctcacgc gaagacgcga agacgcgaag aggtttgcgg cgatgtcttt cgcggcttcg
     2101 cggcttcgcg gcttcgcgtc ttcgagtgag cgtttcacgc gccgaacata tcgcgatagt
     2161 gccggtacca atcgctttga aagcgttcgt tcggatcgta gcgtcgcttc atcgccagga
     2221 attgcgcgaa ctgcggatag cagctctcga cctgatcgcg ccgggcgaag cggtgataag
     2281 tgaggtagta acttccgccg cgggcgatgg ccatatcgat gagtcttcgg aacgcgtcgg
     2341 cggatgattg caagccgtcg atcgtgtgtt cgacgtgcag attgaaaatc acgcacgcgt
     2401 aggcctcttt cgcccacacg agaaacgact cctcatcctt ctcgatcatg cgaatcgtgc
     2461 cgtagatgtt cggcgtgtga tgcgcaagga aatcggcgcg cacctcgtcc atgaaatcga
     2521 tcaggcgctg ccggggaacg tagacctcgg tgatcatctc ggtggcgcgc gagccggttt
     2581 gcgcatcgac cgcatggtgg tagtcgtcga tgtaaaggcc cgactgatgc gtgtcggatg
     2641 gatagatctg gccgctcgtg ctctggtagt acgtgctgta gacctcgtag acgcggcggc
     2701 ggtcgaagtg agcgagagcg atgagctcct cccaatcgcc gacgcccagt tcacgcgcgg
     2761 ttccgagcgg caaatcggtt gggcggtagc aggcgaagac gccctctctc atgaaccccg
     2821 gcgatttctc atcgatcgag aattggaagt cgccgtagag atagccgtca gcgattcttt
     2881 gctggatcgc ctcgataatc ccttcgatgt tgcggatctc gacgacgcgc tcgacgcggc
     2941 tgcgaggacg gaggcggagg gcgacggagg tgatgacgcc gaagagtccg tagccgccga
     3001 tggcgagcga gaagagtttg cgattctctt cgcggctgca gcgccgcgct tcgccggcgt
     3061 ggtcgatcag cgtgaaggcc tcgacatcat tgatgatcgg cggccagatc aggccgcgtc
     3121 catgaacgtt ggacgcgagg gcgccgccga tcgtgagccg gtcggcgcca gtctgcttct
     3181 gaacgatgca ccacgaggat tcgctccctt cctgcatcaa ctccagtccg cgcatcaact
     3241 ccggccactg aatccccgcc tccacctcgg ccactcctcg cgcggcatcg agagtgacga
     3301 tgcgattcag cgcacgcatg tcgagatgca cggtcccagc accgaactgc tgtccgccca
     3361 tcgagtgccg tccgccggag atggagatgg atttctcata gcgcgcggcg tcaagaaccg
     3421 cgttctgcac ggcctcctgc gaagcaacag agatcacgcg atccacgcgc gtctcattca
     3481 gttgcgagtg aatgtcgttg aggagcacgg acgtcatgtt gcgcgaaagt cgcgcttgcc
     3541 gtcgttccgt tcgggtggcg gaacggtcac ttacttgcga agatggcgtt gctcagctcc
     3601 gacgttaccg ccgcgtcgtg gtagtacggg tcggaagcgt tcgggatgat gcgcgtccag
     3661 gtggcgttga tccactggcc gcgaagatca ccacccacag acacggtgat cgtaccggtc
     3721 gtggccgaac tggcgtgcac ggaccctaga gtcttctctc cctcgccgtc cggcgcgccg
     3781 ttgccgtaga agtcgatgac tccgtcttgc tgcgtggtgg tactcagcgg tgtgctcttg
     3841 atcgagagag tgatgaacgt ccggttctgc gcggcatcgt aggtcgccga gagaagcacc
     3901 ggcgcgtttg agggtccatc cttgtcgttg ccatcgacag gtgagactcc atccaggccc
     3961 cagtcgatgc cgaggccgcc gttgttcttc atggtgttgc tgtgaatctc gacgtgcgct
     4021 ttgctgcggg ccaccgccac gcccatgtcg cgatgatttg aaatcgtgtt tcctgtaatc
     4081 gtgatgtcgt cgacacctgg accgaggaaa atcccgcttg ctccgttgct ttcgatgcga
     4141 tttgtctcga attgtgccga tccgttccac atccagacgc cgctatttcg gttgtgactg
     4201 atcacgttgt tcgagacgag aacgtttgcg ccgccggcat tgagaccgcg cagattcggc
     4261 cacgcggtcg tacccgtcgg atcgacgccg atgtaattgc tcgccacagc gcgacggtcg
     4321 ggacgaaacg cttccggaca gccagcgccg ccgccgaacc acaatccctg atcctcatca
     4381 aaattgccca gagcgagacc ttcgatgacc gcttcgcatc gcgcgacgat cctcaatccg
     4441 cggcgggcca gacggccatc gatggcaatc tctggcccga gaggattcgt atcgccggtg
     4501 aatgctttct gccgcagacc ttctacgctg acacgatccg cggtgatcgt gggcagcggc
     4561 tccgctggcg tgatcgtgaa ccatccctcc gccggtacgg gcggcggaat ctcgaagacg
     4621 atgcggcacg gaccgggagt gcagttggcg ttcgcctgga cgatggcatc gcgcaacgag
     4681 cccgggccgt cgtcggcgac cgtcgtgacg gcgtaccaac gatagatggg aacggtgttc
     4741 tccgtctggt cgttggccgg cgtcttgtcc ggtacgtcgc ccgcggcgcg catcgacagg
     4801 gtggcctttc ctgccagccg gtctgctgtc gttttcaccg tgaccgtgat cgtgttcgga
     4861 ttctccacaa gaggcgagat cgtacataca gcggtggcgc cagcgatcga gcacgagact
     4921 ggagaaggtg cgtcgatctt ctcgatcacg ccgttcgtga ccgcgtagtc gatgcgcacg
     4981 ttcggtggtt tattgccgcg aacattgttg ccaacgtacg tccagaactc cgcgttctgt
     5041 ccgggatcga tgcgttcgaa gcggggcgaa cggtcgatca gaacaccgag atccgcctca
     5101 agcttcgttg tgagcatgac ggacgccgaa ttcgtggaag gtgtagggtc tggcgtatcg
     5161 gaagaaatcg tgaatgtcac tttgtagctg gcgtccacga acggcccctt gaagttcgcc
     5221 tccaggtagt acgcaaacgt attcgtctca ggagcgaaga cgggaatgtc gccgagcgag
     5281 cagcggacag ggcgttgggt atagtcgcaa gggccataca cgctgacgac gcgatcgaga
     5341 accagctccg gcggaaagtc gatcgtgagg aagctgtttc gtgctacatc cggaccgtga
     5401 ttcacgacgg ccatcgacac ctgcagaggg ttcgccgcgt acgcttgcat gtcgaccttc
     5461 gctgagtccg cgaacatggt tgtggcgaag agagaacagc cgaggaagaa ggtcgagatc
     5521 cgggtcatga gggatacctg cacgcacact acgtacatc
//
LOCUS       DATLVW010000457         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_430567, whole genome shotgun sequence.
ACCESSION   DATLVW010000457 DATLVW010000000
VERSION     DATLVW010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430567"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VNN25_13900"
     CDS             <1..568
                     /locus_tag="VNN25_13900"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXH92671.1"
                     /translation="IRDNRIRIADYFEAAPKDLLSAVREQRLEGIIGKRKDSLYQPGK
                     RSGAWIKYRVNKGQEFVIGGYFPGTHGIDSLIVGYYDGDKLMYVARTRNGFVPASRRQ
                     VFSKLKHLVTLTCPFVNLPETRRSRFGEELNAEKMKKAVWLKPEAIAQIEFLEWTEGN
                     RLRHSKFVGLREDKNPRDVVKEQLGAEV"
     gene            607..1491
                     /locus_tag="VNN25_13905"
     CDS             607..1491
                     /locus_tag="VNN25_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXH92672.1"
                     /translation="MDIYLIDGTYELFRYFYAVPSSKNASGQEIGAVRGVLGSILTMI
                     EGGATHVGVATDHVIESFRNDLYRDYKTGEGVDPELLSQFPILEKALQSMGVLVWPMV
                     KFEADDALASAAAKAAKDKRVGRIIICTPDKDLSQCVVGSRVVQLDRRRESVRDEAGV
                     IAKFGIKPSSIPDYLAVVGDTADGYPGIVGWGAKAAAAVFSRCPHLEDIPKDWHQWDP
                     TLRRARPLAEALFAGWDDALLFRTLATLRVDVPVFRSIDELRWRGPAKDFKRTCERIK
                     APDLFRRASEKENIVRKR"
     gene            complement(1528..2496)
                     /locus_tag="VNN25_13910"
     CDS             complement(1528..2496)
                     /locus_tag="VNN25_13910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXH92673.1"
                     /translation="MKAIVATNQAAGTAGMKLMERPEPQAAINDVVVQIHASGFTGDE
                     LTWPSTWIDRAGRDRTPSIPGHELAGVVTALGYGTTGLSIGQRVFGLTDWYRDGTLAE
                     YVAVEARNLTPLPGDVDFTVGAALVMPGLTAWQGLFEHGRLHAGQRVLVHGAAGVVGS
                     MASQLAREAGAYVIGTGRAAARQTALDFGAQEFVDLDTDALEDIGEVDLVFDVLGGDI
                     AKRSAGVIRAGGTLVTVTGPTEARPSGGRAIDFVVEPDRAQLSELVQRVRVGRLRTNI
                     GEVAALDDAVAAFNRTERIKGKTIIRVHPARGLPKSIQETRNAASA"
     gene            complement(2556..2648)
                     /locus_tag="VNN25_13915"
                     /pseudo
     CDS             complement(2556..2648)
                     /locus_tag="VNN25_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT          556 a          763 c          835 g          538 t
ORIGIN      
        1 gatccgcgac aatagaataa ggattgccga ttatttcgag gcagcgccaa aggacctgct
       61 gtctgcggtg cgcgagcaac ggcttgaagg gattatcggg aaacgaaagg atagtctgta
      121 ccaaccggga aagcgcagcg gcgcctggat caagtatcgg gtgaataaag ggcaagagtt
      181 cgtcatcggt ggctattttc ccggaacgca tggcattgac tcactgatcg tcggctacta
      241 cgacggagac aagctaatgt atgtggcaag aactcggaat ggatttgtcc cggcttcgcg
      301 gcgacaagtg ttttcgaaat tgaagcatct tgtgacgctg acctgcccat tcgtaaattt
      361 gccggagacg agacggtcac gatttgggga ggaactcaac gccgagaaga tgaagaaggc
      421 ggtgtggttg aagccagaag ccatcgctca gatcgaattc ctagaatgga ccgagggaaa
      481 ccgattgcgt cattcgaagt ttgtcggact gcgcgaagac aagaatcctc gcgatgtcgt
      541 taaggagcaa ctgggcgcag aggtgtgaaa cagcctcggg gcggcatcgc cagctgagtc
      601 gagttcatgg atatctatct cattgacggg acgtacgaac tatttcgcta cttctacgcc
      661 gtaccgtcgt ccaaaaacgc ctccggccag gagattggtg cagtccgcgg cgtgcttgga
      721 tcgattctca cgatgattga gggcggagcg actcacgtcg gcgtcgcaac cgaccatgtg
      781 atcgagtcgt ttcgcaatga tctttatcgg gactataaga ctggcgaagg tgtggatccg
      841 gaactgctct cgcaatttcc aattcttgaa aaggcgctgc aatcgatggg agtgctggtg
      901 tggccaatgg tcaaattcga ggccgacgat gcgcttgctt cagctgcagc gaaagcagct
      961 aaggataagc gcgtcgggcg gattatcatc tgcacgcccg ataaggacct cagccaatgc
     1021 gtcgtggggt cgcgagtcgt acaacttgac cgccggcgcg agagtgtccg tgacgaggct
     1081 ggcgtgatcg caaagttcgg gatcaaaccg tcgtcgattc cggactatct ggctgtggta
     1141 ggcgacactg cagacggcta tccgggtatt gtgggatggg gggccaaagc agcagcggcg
     1201 gttttctcgc gatgtccaca tctggaagac attccgaaag actggcatca gtgggatccg
     1261 acgttgcgga gagcgcgtcc ccttgctgag gcgcttttcg ctggctggga cgatgcacta
     1321 ctgtttcgca ctctcgcaac acttcgagtc gatgtcccgg tgttcaggtc aattgacgag
     1381 ttgcggtgga gagggcctgc caaggatttc aagcgcacct gtgagcgcat caaggcgcct
     1441 gacctgttcc ggcgtgcctc ggagaaggag aacattgtca gaaaaaggta agcgtacaag
     1501 cagagccgtc tgaacggagt gtcaaggtta ggcggatgca gcgttgcgtg tttcctgaat
     1561 tgacttaggc aatccccgag ccggatgaac gcggatgatt gtctttccct tgatccgctc
     1621 ggtccggttg aaggcggcaa ccgcatcgtc gagggcagcc acttcgccga tgtttgtccg
     1681 cagccgacca acgcggaccc gctggacgag ctcactcaat tgggcgcgat cgggttcaac
     1741 gacgaagtcg atagcccggc cgccagaggg ccgcgcctca gtcgggcccg tgacggtcac
     1801 cagcgttcct ccggcgcgaa tcacgcctgc agagcgcttc gcgatgtcgc cgccgaggac
     1861 atcgaaaacc agatcgacct cgccgatgtc ttccagggcg tcggtatcga ggtcgacgaa
     1921 ctcctgtgcg ccgaagtcga gagccgtctg acgggcggca gcgcgcccgg tgccgatgac
     1981 gtacgcgccg gcctcacgtg cgagctggct cgccatcgaa ccgactacgc cggccgcacc
     2041 gtgcacgagg actctctgcc ccgcgtgaag gcggccgtgc tcgaacagcc cctgccacgc
     2101 ggtcaggccc ggcatcacga gggccgcgcc caccgtgaag tcaacgtcgc ctggcagcgg
     2161 tgtgaggttg cgtgcctcga cggccacata ctccgccagc gtaccgtcgc gataccagtc
     2221 ggtgaggccg aacacccgct gcccaatcga cagccccgtc gtgccatagc cgagagcggt
     2281 gaccactccg gccagttcgt gtcctgggat tgaaggcgtc cggtcacggc cggcgcgatc
     2341 gatccaggtc gagggccacg tcagctcatc cccggtgaat cccgacgcat gaatctgaac
     2401 aacgacgtcg tttatcgccg cctgcggctc gggccgctcc atcaacttca tcccggccgt
     2461 tcccgcggcc tgattcgtcg ctactatcgc cttcatcggt cgcctctctg tgtcgagttc
     2521 ttgcctgtaa ggtagccgct ctatccgatg tccctggacg ggttaccgga gagaaacgga
     2581 tcgatcaaaa tcttggcctc gcccgcctcg atgcgaaatg cggaaaggcc gtgccatgtc
     2641 agtttcatag gaattcccag ctgcccgggc cttagttgca acgttctgct gt
//
LOCUS       DATLVW010000458         3517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_598322, whole genome shotgun sequence.
ACCESSION   DATLVW010000458 DATLVW010000000
VERSION     DATLVW010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598322"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNN25_13920"
     CDS             complement(<1..415)
                     /locus_tag="VNN25_13920"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HXH92674.1"
                     /translation="MSTVRTWRLVKSRFVEHAFDGEGSRLYGGRWSSPGVPVVYTSAT
                     ASLAVLEIFANVQHQDLLASFELLSCAFEDALVSSVEPSELPTNWRQSPAPARLQEIG
                     DEWLRTARSVVLQVPSSIIEHESNYLLNPLHPDFKR"
     gene            complement(412..840)
                     /locus_tag="VNN25_13925"
     CDS             complement(412..840)
                     /locus_tag="VNN25_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HXH92675.1"
                     /translation="MNEGARAHIHVVLLGVKASDWTELMRIAARGLTYDALEHFQRNT
                     GIAYERLLAWIQISPRTMARRKQQRRFLPGESDRLLRASRVFGKALELFEGDRDAASE
                     WMLTPQPALGGETPIDVAQTELGSREIENLIGRIEHGVYS"
     gene            complement(957..1400)
                     /locus_tag="VNN25_13930"
     CDS             complement(957..1400)
                     /locus_tag="VNN25_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXH92676.1"
                     /translation="MTDWREVPVFGNAPKETSAKVRPSAYALIENDRGQFAIVRTKEG
                     TYLPGGGIDAGETPGEAVIRETLEECGLSVRIGSLIAEAVQFAWSEAEETYFEKRSTF
                     VDAEVLGPDTSRLEADHEVLWVDAETACTMLTHESHAWAVRRRTG"
     gene            complement(1400..2434)
                     /locus_tag="VNN25_13935"
     CDS             complement(1400..2434)
                     /locus_tag="VNN25_13935"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXH92677.1"
                     /translation="MSNESEILHVGIDLGTSRSAISASNGERFVVDSFVGWPADMVAR
                     KVLKRDVLIGHEAVANRTMLDLHRPLERGLLKEGSDKDVEAVRELLKHLLRLVGVSSN
                     GKIDSSNVRAVVGVPAASLRTNKQYLRNSMKGIVDSLMIVSEPFAVAYGLNALLHTMI
                     IDIGAGTTDFCVMKGRYPGEEDQRTLTNAGDSLDTQLLKLIEERYPQANVTIFMVREW
                     KEKHSFVGEAPSRVLVTAPVKGVPTELDITNEMKAACETLVPPTIETMLDLLSRVEPE
                     FQERVRNNVILSGGGGLIRNLSTTLEAALKQVGGGKVTHMKDPVFVGSDGGLALALDA
                     PDEDWEKLTA"
     gene            complement(2434..3459)
                     /locus_tag="VNN25_13940"
     CDS             complement(2434..3459)
                     /locus_tag="VNN25_13940"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXH92678.1"
                     /translation="MRAGNIERVKRQALREAVAENLDTIRGLPPRRLRPGYRTFRIFL
                     RRAPLLIIPLMLAGFAYLTSTSVPAAATVKVAARSLPSQPVQRASVLTPAPALLTDSL
                     ETVSASAFPLSVRRVVLDAGHGGTDPGASSISNVSEKDITLDIEQRLGTMLRKNGFEV
                     VSTRGDDRLIPLRERAHLANSSKSDIFVSIHVNSIPQGTNHGVETYYLGPTNDPSLTQ
                     LAAKENLTSGYALADLRKLLDRVYADVRRDESQRLASVVQHQLFSGLRGVDPGLENWG
                     VKRAPFIVLVATDMPAILAEVGCLSNEREAEMLRSSAYREQIAQALFHGINAYARAND
                     APQRKGT"
BASE COUNT          607 a         1063 c         1138 g          709 t
ORIGIN      
        1 cacgtttgaa gtcgggatgc agcgggttca gcaggtagtt gctttcgtgt tcgatgatgc
       61 tgctcggcac ctgcaacacg accgacctgg cggtgcgcag ccattcgtcg ccaatctcct
      121 gaagccgcgc gggggctggt gattgtcgcc agttcgttgg caactcggac ggttcgacgc
      181 tggatacgag cgcgtcttcg aacgcacacg aaagaagctc gaagctcgcg agaaggtctt
      241 ggtgctggac gttcgcgaaa atctcgagca ctgccagcga cgcggttgct gatgtgtaga
      301 cgacagggac ccccggcgag ctccagcggc ccccgtagag ccggcttcct tcgccatcaa
      361 atgcatgctc gacgaatcgt gactttacga ggcgccacgt tcggaccgtg ctcacgaata
      421 gacgccgtgc tcaatgcggc cgatgagatt ctcgatctcg cgcgagccaa gctcagtctg
      481 tgcaacgtcg atcggtgttt cgccgccaag tgctggttgc ggggtgagca tccactcgga
      541 tgcggcgtcg cgatccccct caaacaactc caacgccttt ccgaatacgc gtgaggcgcg
      601 taacaggcgg tcagactccc ctggcaagaa acgtctctgc tgttttcgtc gcgccatcgt
      661 acgtggcgag atctgtatcc acgcgagcag tctctcgtag gcgatgccgg tgttgcgctg
      721 aaagtgctcc agtgcgtcgt acgtcaagcc acgagcggcg attcgcatca actctgtcca
      781 gtcggatgcc ttgacgccca ggagcacaac atggatgtgt gctcgagcac cttcattcat
      841 gaggctcgtg aagtgagcca gatccttcga tggtttcgga ccagactggc gatgcgtcgt
      901 cgaggttgcc atatgtcaac tggcacaatt atcctcgcca cctggctgaa agtcaattag
      961 ccggtccgtc tcctcaccgc ccaggcgtgg ctctcatgcg tcagcatcgt gcatgcggtt
     1021 tcggcgtcga cccacagcac ttcgtgatcc gcttccagcc gcgaggtgtc gggtccgagc
     1081 acctcggcgt cgacgaacgt ggagcgcttt tcgaagtacg tttcctctgc ttccgaccag
     1141 gcgaactgca ctgcctccgc gatcagggag ccaatgcgca cggaaaggcc gcactcttcc
     1201 agcgtttccc gaatgacggc ttcgcccggc gtctcgcccg cgtcgatacc gccgccgggg
     1261 agataggttc cttccttcgt gcgaacgatt gcgaattgtc cgcgatcgtt ctcgatcagc
     1321 gcatacgcgc ttggccggac cttggcggat gtttccttcg gcgcgttccc gaagaccgga
     1381 acttctcgcc agtcggtcac tacgccgtca gcttttccca atcctcgtcg ggggcgtcga
     1441 gggccagcgc caggccgccg tcggagccga cgaagacggg atccttcatg tgcgtcactt
     1501 tgccgccgcc gacctgtttg agcgcggctt cgagcgtcgt ggagagattg cgaatcaggc
     1561 cgccgccgcc ggagaggatt acgttgttgc gcacgcgctc ctggaactcc ggctcgacgc
     1621 gcgagagaag atcgagcatc gtttcgatcg tcggcgggac gagcgtctcg cacgccgctt
     1681 tcatctcgtt cgtgatgtcg agctccgtcg gaacgccctt gaccggcgca gtgaccagca
     1741 cgcgcgacgg cgcttcaccg acgaagctgt gtttttcctt ccactcgcgc accatgaaga
     1801 tcgttacgtt cgcttgcgga tagcgctctt cgatcagctt gagcagttgc gtgtcgagcg
     1861 aatcgccggc gttcgtcagc gtgcgctgat cttcttctcc cgggtatcgg cccttcatca
     1921 cgcagaagtc ggtcgtgccg gcgccgatgt cgatgatcat cgtgtgcagc agcgcgttca
     1981 atccatacgc gaccgcgaac ggctcgctga cgatcatcag actgtcgacg atgcccttca
     2041 tcgagttgcg caggtactgc ttgttcgtgc gcaacgacgc ggcaggaacg ccgacgaccg
     2101 cgcgcacatt cgagctgtcg atcttgccgt tcgaactgac gccgacgaga cgcagcagat
     2161 gcttgagcag ctcgcgcacg gcctcgacgt ccttgtccga gccttccttc aacaagccgc
     2221 gctcgagggg acggtgaaga tcgagcatcg tccgattggc aaccgcttcg tggccgatga
     2281 ggacgtcgcg tttgaggact ttgcgggcca ccatgtcggc gggccagccg acgaaactgt
     2341 cgacgacgaa gcgttcgccg ttcgatgcgg agatcgcgct gcgggaggtg ccgaggtcaa
     2401 tcccgacgtg gaggatctcg gattcgttgg acattaggtt cctttcctct gcggcgcgtc
     2461 gttcgcgcgg gcgtatgcat tgattccgtg gaagagagcc tgcgcaatct gctcgcggta
     2521 ggcgctgctg cgcaacattt ccgcctcgcg ctcattcgaa agacaaccca cctcggcgag
     2581 gatggccggc atgtcggtgg cgacgagcac gatgaacggc gcgcgcttga cgccccagtt
     2641 ctcgaggccg ggatcgacgc cgcgcaggcc gctgaatagc tggtgctgca cgacgctggc
     2701 gaggcgctgc gattcgtcgc ggcgcacatc ggcgtacacg cggtcgagca gcttgcgcag
     2761 atcggccagc gcgtatccgg atgtcaggtt ctcttttgcg gcgagttgcg tgagcgaagg
     2821 atcgttggtc gggccgaggt agtaggtctc gacgccgtgg tttgtgccct gggggatgga
     2881 gttgacgtga atggagacga agatgtcgga cttggagctg ttggcgaggt gagctcgctc
     2941 gcggagcgga atcagccggt catcgccacg cgtggaaacg acctcgaatc cgttcttgcg
     3001 caacatcgta ccgaggcgtt gttcgatgtc gagggtgatg tccttctcgc tgacgttcga
     3061 tatggacgat gcacccggat cggtgccgcc atgaccggcg tcgagaacga cgcggcggac
     3121 ggatagcgga aatgccgaag cgctcacggt ctccagcgaa tccgtcaaca acgccggcgc
     3181 cggagtgagg accgacgcgc gctgcaccgg ttgcgacggg agtgatcgcg ctgcgacctt
     3241 taccgttgcc gcggcgggga cgctggtgct ggtgaggtag gcgaagccgg cgagcatcaa
     3301 cggaatgatg agcagcggcg cgcggcgcag gaaaatgcgg aacgtcctgt agcccggtcg
     3361 cagacggcgg ggtgggaggc cgcgaatcgt atcgagattc tccgctaccg cctcgcgcaa
     3421 cgcctgccgc ttcacccgct cgatgttccc cgctctcatt ccgctcattc tacgggattt
     3481 gggggtgggg gagtggggag tggggtgtgg ggagtgg
//
LOCUS       DATLVW010000459         7251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_18951, whole genome shotgun sequence.
ACCESSION   DATLVW010000459 DATLVW010000000
VERSION     DATLVW010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18951"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VNN25_13945"
     CDS             complement(<1..211)
                     /locus_tag="VNN25_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92679.1"
                     /translation="MATIRTQALTIERLRQILDPGRVFYRVAVDDVLKRGDLVEIQTL
                     IKGARDAKAKFGDFDGLIKTLEAAAE"
     gene            521..913
                     /locus_tag="VNN25_13950"
     CDS             521..913
                     /locus_tag="VNN25_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92680.1"
                     /translation="MNRPGWYTEEQDNGWQRVKAAFGNDWEQTRHDFGSNTARELNQD
                     AGDTVKQAIGADDAFENHEQAFRFGYAAQSHYKKEYPTWNDDLDTQLRKDYGTDYDRD
                     RSDIRRAYEYRYAGTAGSGLRDENRDRP"
     gene            complement(1035..1718)
                     /locus_tag="VNN25_13955"
     CDS             complement(1035..1718)
                     /locus_tag="VNN25_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH92681.1"
                     /translation="MSLVALVTGSSRGIGFEVARQLAVRDFTVIVTSRSERAAAAAAK
                     KIGGSAVGHILDMSDPASINGIADWITREVGHLDVLVNNAAILLDESETILATPAETF
                     ETTMRTNALGPLLMTQASAPLLRRSTAARVVNVSSGAGQISSMTSYAPSYSISKTALN
                     AITVMLASALPFARVNCADPGWVRTDMGGPNATRSVEEGADTIVWLATLPASGPSGGF
                     FHDRKRIDW"
     gene            1942..2133
                     /locus_tag="VNN25_13960"
     CDS             1942..2133
                     /locus_tag="VNN25_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92682.1"
                     /translation="MSDRTKGEVSETGSEHHSKAKGAAIGAVAGGMLGGKKGAVVGAA
                     IGAEKQHRKNENEEDSRKK"
     gene            complement(2290..3846)
                     /locus_tag="VNN25_13965"
     CDS             complement(2290..3846)
                     /locus_tag="VNN25_13965"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HXH92683.1"
                     /translation="MTWHAAKFLAFNSALLIAAAFAFFLFAAAPHRGETWLRQWGSRF
                     RQHPVFGAFVRKHFISHERSLVRLSIGIVVVAASGYEFVHILQGVLTDSKLTAADVRL
                     HNTLRLFHSEALHRSYSAVTRLANAWFLVPLSIALAGLMYAARRRRDALVLVAATFLP
                     TIFAVVLKYLVNRPRPIEARPFVSGPSFPSGHTLMATVVYGFIAYVILADAPKRRLAF
                     VAVLALLTFIVLVPLSRVYLGVHWPYDTLASLALGVALLAVLIEAMKSERLARLQHDS
                     PPWFAKSVAVFAGLTIIAAGFYATASVEKEVRPLSPQPVGVVSIDLLRQNFPPALKRT
                     SEDLVGGPMEPAAFVFDGDAAALVRSFQRAGWSLADSPSATGLLHELLCVIRDAPDPR
                     GPATPSYYDGQPQDLTFERPATATGSIRHRHHIRVWREPLNILPAVPLWVATCSYDEG
                     VKLVPKPYLLTHRIDPRVDEERELIASELRNAGVQDVVLVIVTGPRHGTNAGGDAFIT
                     DGRAHVMIFP"
     gene            complement(4240..7089)
                     /locus_tag="VNN25_13970"
     CDS             complement(4240..7089)
                     /locus_tag="VNN25_13970"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92684.1"
                     /translation="MLGKHYEVIEVLSGGMGEVFICEFIGDDDDPGPRARMALKTFQQ
                     RFFFDNAVRNAFVREASVWLRLTGLPFILPALGIEQIDRRPFIRMPAVDSRNGVRTLA
                     DLVARGDLPPLDALHFAFQIALGMRQAVERVRDLVHGDLKPANVLMMGGKAFVSDFGL
                     ATAAALGRADTRLVGTPAYRAPELAAEGAAPTSASDVYAFGASLAEMMANVLPGETIG
                     AAGLELARRCMDAVPLKRPSGFAEILQHLSGLLHEHDPAGLIMRMADAIQYRELFHQP
                     ESASLHVERVGSLIELGETRQAIEELDAIPREKYTPELWLRRAMALSLENRPEDAIEA
                     LGPALENEAQLEPQSRVLAHSEYALCLKRLGRLDEAERIYRDLLPEVDDEHLSIVVIN
                     LATVYLQQRKAEEAVRLLEPFTKKQPNFAQGWANLGRAYMDTGRYEDAENALGRALQR
                     DPSAGVVRVWLAELYMEHLRRVPEAWAALDAAFDAGYESREWLVRFLACSLLLERKDT
                     LANLKHAIDNNFEKELADSMVRDAIEMAKRIAGGGDDDDPAPAASEVVPERPPEPEDY
                     TMAEPVSEPLPASEPNARSDRRSSTPFINFRYYATGDFTIDFYEDPHNPNFLDAFKRE
                     WRRALRDPGMQLQGASLRGSPFYFTRCPVCGVYVITNREIGYKLDCRMCDTRWFTSAL
                     DDPSLSELTQHVTELTGIVDAEKSLGGIDALLLQAKRAEEQARADEICREAGMTLSPP
                     DQYLTVAVLRQAVDHGVCDLRLPWSVWTMPAEGRSWARDATPPDVGRVVRKLQEALPG
                     IRSLSTTIPAESVEALSGSVAEASARDEQQIREKLRRNDASAEEMRRLASILAQRGDQ
                     LGEAETIARAALAADDTSADGWQVLGWIFVRAGKFGAARALLERALALDPTRVLNWRM
                     LAVCCEKTGDLQGAAEANLRATAITGDSQPLFPSE"
BASE COUNT         1421 a         2229 c         2303 g         1298 t
ORIGIN      
        1 tctcggcggc cgcctcgagc gtcttgataa ggccgtcgaa gtcgccaaac ttggctttgg
       61 cgtcgcgcgc gcccttgatc agagtctgga tctcgacgag atcgccgcgc ttgaggacgt
      121 cgtcgacggc cacgcggtag aagacccgtc ccgggtcaag gatctggcgg agtctctcga
      181 tcgtgagtgc ctgcgtgcgg atcgtagcca tagttcgtcc tcctcctggt tggatgtctc
      241 tcttagttta ctgatctaaa gaaggatgcc cactctcttt tggattgtca agagcgccgc
      301 gtcgtggtgc gatcgaggtc gtcggtgatc acttttttgt cgaagaaggc cggtgggctc
      361 gatgtctttg cgagcgactg aaaacacgaa acgccggcga gatgctacgg cgggagcagg
      421 cgatgcccac gttgcggccc ggcggaagaa cagcacactc gtggcgcgcc ccttgctctt
      481 tcgttctgct gtagcgacaa caatgaataa ggagaaatca atgaaccgtc caggttggta
      541 tacggaagag caggacaacg gttggcagcg cgtcaaggcc gcgtttggca atgattggga
      601 gcagaccaga cacgacttcg gatcgaacac cgcgcgcgag ctcaaccagg atgcgggcga
      661 cacggtgaag caggctatcg gcgcggacga cgcgttcgag aaccacgagc aggcgttccg
      721 tttcggctac gcggcacaga gccattacaa gaaggagtat ccgacgtgga acgatgacct
      781 cgatacgcaa ttgcggaagg actacggcac ggactacgac cgtgaccgca gcgatatccg
      841 ccgcgcctac gagtatcgtt acgcgggcac ggcaggctct gggcttcgcg acgaaaatcg
      901 cgatcgcccc taagacagtt cgttcgtcat gattagaaaa gagaggcgcg acgcctctct
      961 tttctatctc ctgtcactct gacgaaggca gagctttcgc gtcattacgt tccgcggtaa
     1021 cagtaaatcc cgggttacca gtcgattcgt tttcggtcgt gaaagaaacc gccgctggga
     1081 ccgctcgctg gcagcgtggc cagccagacg atcgtatcgg ccccttcctc gacgctgcgc
     1141 gtggcgttcg ggcctcccat atccgtgcgc acccaacccg gatcggcgca gttcacacgt
     1201 gcgaagggaa gggcggaagc gagcatcacc gtgatggcgt tcaatgccgt cttcgagatt
     1261 gagtacgacg gcgcatacga agtcattgac gagatctgcc ccgcgccgga ggaaacgttt
     1321 acgacgcgcg cggccgtgct gcggcgaagc aggggagcgg atgcctgcgt catgagcagc
     1381 ggcccgaggg cgttggtgcg catggtggtc tcgaacgtct ccgccggcgt tgcgaggatg
     1441 gtttcgctct catcgagcag gatcgccgcg ttgttgacga ggacatcgag gtgtccgacc
     1501 tcgcgcgtga tccagtccgc gatgccgttg atcgacgccg ggtccgacat atcgagaatg
     1561 tggccaactg cagacccgcc gatctttttc gccgctgccg ccgctgcccg ctcggatctg
     1621 ctggtcacga tcacggtgaa atcacgcacc gcaagctgcc gcgcgacctc gaatccaatt
     1681 ccccggctcg atccagtgac gagtgcgacc aggctcattc gttctccttc cccacggtgc
     1741 tgccagcatc gcggatcatg cccgccattc aacttgtgaa ccaggtttcc tcatcaacca
     1801 ccgcgttcca tctcttcgac ggcaacacgc ggtacgcgcc gaggccggca aggttcaccg
     1861 aggggccggt gaggaagcgt aggcatttag gttgctctcc aggcaggtgc acgcggaggg
     1921 atccgcatcg ggaggaaagc catgtctgac agaacgaaag gcgaagtgag tgaaacagga
     1981 agcgaacatc acagcaaggc caagggcgca gcaatcggcg ccgtcgccgg cggtatgctc
     2041 ggcggcaaga agggcgctgt agtgggcgct gcgatcggag cagagaaaca gcaccggaag
     2101 aacgagaacg aagaggattc gcgcaagaag tgatgtttcc cgagaagatc gatcatcacg
     2161 tccacgcctg gatcaaagcc aggcgttcgg ccgaagcgga acgcgtcgtt cgcggtttct
     2221 cgcaagtggg aagctccgcc gtggtgctcc cgatcacggc gattgccgcg acgttgatgc
     2281 ttcggccact cacgggaaga tcatcacgtg cgctcgtccg tcggtgatga acgcatcgcc
     2341 tccggcgttc gtaccatggc gcggtcccgt cacgatgact aacacgacat cctgcacgcc
     2401 ggcgttgcga agctcggaag cgatcaactc ccgctcttcg tcgacgcgcg ggtcgatgcg
     2461 gtgcgtgaga aggtagggct tcggaacgag cttcactcct tcgtcgtagc tgcaggtcgc
     2521 aacccacaaa ggcacagccg gcagaatatt gagaggttcc cgccacacgc ggatgtgatg
     2581 acggtggcgg atcgatccgg tcgccgtggc gggccgctca aacgtcagat cctgcggctg
     2641 gccatcgtaa taggacggtg tcgcggggcc gcgcggatcg ggagcatcac gaatcacgca
     2701 gagcagctcg tgcagaagtc cggtagcgga tggtgaatcg gcgagagacc acccggctcg
     2761 ctgaaagctg cggacaaggg ccgcggcatc cccatcaaac acgaatgcgg cgggctccat
     2821 gggacccccg accaggtctt ccgaggtccg tttcagcgcg ggagggaagt tctgtcgcag
     2881 aagatcgatc gacaccactc ccacgggctg cggtgaaagg ggccgcacct ctttttcgac
     2941 gctggccgtc gcgtagaagc cagccgcgat gatagtgagt ccggcgaaga cagccacgct
     3001 cttcgcgaac cacggcgggc tgtcgtgttg cagccgcgcc aaccgctcgc ttttcatcgc
     3061 ctcgatgagc acggccagca gcgccacacc aagcgccaga ctggccaacg tgtcatacgg
     3121 ccagtgcact ccgagataca cgcgcgagag cggtacgagg acgatgaagg tcaggagagc
     3181 cagcacggcc acgaatgcca gccggcgctt cggagcatcc gcgaggatga cgtatgcgat
     3241 gaatccatag accaccgtcg ccatcaatgt gtgtccggat ggaaagctcg gcccggagac
     3301 gaagggacgg gcctcgatcg gtcgcgggcg attcacgagg tacttcagca ccacagcaaa
     3361 gatggtcgga aggaaggtcg cagcgaccag aaccaaggcg tcgcggcgcc gtcgggcggc
     3421 gtacatcagt cccgccagtg cgatcgaaag aggaacaaga aaccaggcgt tcgcgagccg
     3481 tgtcaccgcc gagtaggagc ggtgcaatgc ctcggagtgg aaaagccgca gcgtgttgtg
     3541 cagtcgaacg tcggctgcgg tcagcttgga gtccgtcagc acgccctgga ggatgtggac
     3601 aaattcatag ccggatgcgg cgacgaccac aatcccgatc gaaagacgta cgagagatcg
     3661 ttcgtggctg atgaagtgtt ttcgaacgaa ggctccgaaa acggggtgct ggcggaagcg
     3721 gctgccccat tgtcgcaacc atgtctcccc tcgatgcggc gccgctgcga acaggaaaaa
     3781 cgcgaacgcc gccgcgatca ggagcgcgga gttgaaagcc agaaacttcg ccgcatgcca
     3841 ggtcattgtc ggcctcgaac gaaaccgggc gcctcgtcgg ataaatctgt cccagacagc
     3901 gcgcgcgatg cggaacgcgt gcctctcttc cgttcaactt cccggccgct cggaatcgcg
     3961 cgcgcaggtc tggtgaggcg ccactatttg tctgtgggat cgagggcgct cctcgcaagg
     4021 tgacctcgaa agtctggctt cgctccagca gttggcaaga aacgcaccga ctttcagatt
     4081 gccagtagat ctggttcata caatgcgaaa tgatgaagct cgacgacaca acggccggga
     4141 cgaggacgcg cactaactcg atcacgatgg atctgcagtt gaaggtccgc attccggcag
     4201 cggtctcgcc gacggcgagg tgatcgcggg atatcgcgcc tactcagagg gaaacaacgg
     4261 ctgagaatcg cccgtgatcg ccgtggcgcg caggttcgct tccgcggccc cctgcaaatc
     4321 ccctgtcttc tcgcagcaca cggcgagcat cctccagttg agcacgcgcg tcgggtcgag
     4381 cgcgagcgcc cgctcaagca gcgcgcgggc cgctccaaac ttgcccgccc gcacgaagat
     4441 ccagcccagg acttgccatc cgtcggcgga cgtatcgtcg gcggcaagcg ccgcgcgggc
     4501 gatcgtctca gcttcgccga gctggtcccc gcgttgcgcc agaatcgagg caagccgccg
     4561 catctcttcc gcgctcgcgt cattccggcg gagcttttcg cggatctgct gctcgtcacg
     4621 cgccgatgct tcggccacgc tgccgctcag tgcttcgacg ctttccgccg gaatcgtcgt
     4681 cgacagcgaa cgaataccgg gtagtgcctc ctgcagttta cgtacgacac gtccgacatc
     4741 gggcggtgtg gcgtcgcgag cccatgaccg tccctccgca ggcatcgtcc acacactcca
     4801 gggcaggcgc aagtcgcaca cgccatggtc cactgcctgt ctcagaacgg caacggtcag
     4861 gtactggtcg ggcggcgaca aggtcatgcc ggcctcgcgg cagatttcgt ctgccctggc
     4921 ctgttcttct gcacgtttcg cttgaaggag cagagcatca atcccgccga ggctcttctc
     4981 ggcgtcgaca atcccggtga gctccgtcac atgttgcgtg agctcgctga gcgatgggtc
     5041 gtcgagcgcc gacgtgaacc agcgcgtgtc gcacatacgg caatcgagct tgtatcctat
     5101 ctcgcgattg gtgatgacgt acacgccgca gacggggcat cgcgtgaaat agaacggcga
     5161 accgcgtagc gacgcaccct ggagctgcat tcccggatca cgcaacgcgc ggcgccactc
     5221 acgtttgaag gcgtcgagga agttcggatt gtggggatct tcgtaaaagt caatggtgaa
     5281 gtcgcccgtc gcgtagtacc gaaagttgat gaacggcgtg ctggaacgac ggtcggatcg
     5341 ggcgttcggc tcgctcgccg gcagcggctc gctgaccggc tcagccatcg tgtagtcttc
     5401 cggttccggt ggcctctccg gaacgacttc ggaagccgcc ggcgccggat cgtcgtcgtc
     5461 tcctcctccg gcgatgcgct ttgccatttc gatggcatca cgtaccatcg agtcggcgag
     5521 ctctttctcg aagttgttgt cgattgcgtg ctttagattg gcgagcgtgt ccttccgctc
     5581 aaggagaagt gagcaagcga gaaagcgcac cagccattcc cgagactcat atcctgcgtc
     5641 gaacgcggca tcgagagcgg cccatgcctc ggggaccctg cggaggtgtt ccatgtatag
     5701 ctctgcgagc cagacgcgaa caacgccggc gctaggatcg cgttgcagcg cacgtcctag
     5761 ggcattctcg gcatcctcgt agcggccggt atccatgtag gcacggccga ggttggccca
     5821 cccctgcgcg aagttgggct gcttcttcgt gaatggctcg agcagccgca ccgcctcctc
     5881 cgccttgcgc tgctggaggt aaacagttgc cagattgatg accacgatcg acagatgctc
     5941 gtcgtcgact tccggcagca agtccctgta gatgcgctca gcctcatcaa gccgcccaag
     6001 gcgtttcaaa cagagggcgt attcgctgtg cgccagcaca cgcgactgcg gctccaactg
     6061 cgcctcgttc tcgagtgccg gcccgagcgc ctcaatcgcg tcctctggcc tgttctcgag
     6121 cgaaagggcc atggcgcgcc gcaaccacag ctccggcgtg tacttctcgc gcgggatggc
     6181 gtcgagctcc tcgatcgcct gcctcgtttc accgagctcg atcaggctgc ccacgcgctc
     6241 gacgtgcagg ctcgcactct caggctggtg aaagagctcg cggtactgga tagcgtcggc
     6301 catgcgcatg atcaatccgg ccggatcgtg ttcgtggagg agaccggaga gatgctggag
     6361 gatttcggcg aaaccggaag gccgcttcag cggaacggcg tccatgcagc gccgcgccaa
     6421 ctccagtccg gccgcaccaa tggtctcgcc ggggaggacg ttggccatca tttccgcgag
     6481 tgatgctccg aaggcgtaga catcactggc ggatgtggga gcggcgcctt cggcggccag
     6541 ctccggcgcg cgatatgccg gcgttccgac caggcgagtg tcggcgcggc cgagcgcggc
     6601 ggccgtggcc agcccgaagt cggagacgaa cgcctttccg cccatcatca gtacgttcgc
     6661 cggtttgagg tcaccatgca cgagatcacg gacgcgctcg acggcctgcc gcatccccag
     6721 cgcgatctga aacgcaaaat gtagggcgtc gagcggagga agatcgcctc gggcgaccag
     6781 atccgccagc gtacgcacgc cattccgtga gtccaccgcg ggcatgcgaa tgaatggccg
     6841 gcggtcgatc tgttcgatgc ccaatgccgg caggatgaag ggcaggccgg tcagacgcag
     6901 ccacactgat gcctcgcgga caaatgcatt gcgaaccgcg ttgtcgaaga aaaaccgttg
     6961 ctggaacgtc ttcagcgcca tgcgtgctcg cggtccggga tcgtcgtcgt cgccgatgaa
     7021 ttcacagatg aagacctcgc ccatcccgcc ggagagcacc tcgatgactt cgtagtgctt
     7081 gccgagcatc atcggtcaga cgtctcctgt acctgacctg accatgcccg gcgtgccggt
     7141 gtcgggacgc catgactcga tcgtggtgcg aagacgggcg aagccttcgt caccaagcga
     7201 ctgttgcacg gcctcccggg tgtttgaagg agtatcgaga aatccgagaa g
//
LOCUS       DATLVW010000460         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_598590, whole genome shotgun sequence.
ACCESSION   DATLVW010000460 DATLVW010000000
VERSION     DATLVW010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598590"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /gene="guaA"
                     /locus_tag="VNN25_13975"
     CDS             complement(<1..994)
                     /gene="guaA"
                     /locus_tag="VNN25_13975"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXH92685.1"
                     /translation="MSSGQNRHPAANGQTVLVLDFGSQYTQLIARRIREQSVYSVVQP
                     FDISIDRVRALAPQAIIFSGGPQSVYADAAPICSREIFDLGVPILGICYGMQLTAYLL
                     DGKVEASAEREYGRAEIDVRANDSPLFAGTPAHQRVWASHGDNILREPPGFRTTASSA
                     NAPIAAFENIERRCYGIQFHPEVTHSEAGTSILRNFLFEIAHCTPTWNLGDYRKRKVE
                     EIRATVGDKQVIAAVSGGVDSSVAALLVAEAIGDQLTAIFVDNGVLRKNEAIQVMSRL
                     SEGLGMHIIAIDGRQRFYDKLAGVEDPEAKRKKIGNEFIAVFEEEAKRIAGAEFL"
     gene            complement(994..1263)
                     /locus_tag="VNN25_13980"
     CDS             complement(994..1263)
                     /locus_tag="VNN25_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXH92686.1"
                     /translation="MIKADIVNKVAEVSEISRVKAAQAVDTVIEGLRNALARGQRIEL
                     RGFGVFQVKQRKKGIGRNPKTGVEVKIPPGNTIRFKPGKELRNLL"
     gene            1288..1653
                     /locus_tag="VNN25_13985"
     CDS             1288..1653
                     /locus_tag="VNN25_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXH92687.1"
                     /translation="MSDSERHRIDTWLKHVCLVKHRTDATEACRGGHVKLNGQRVKPA
                     APVREGDVVELYAGERFRKFVVKGIPDRQQAKEIARTMYIDETPVIEKVDDSVFRERG
                     AGRPTKRERRDAEKFKRNY"
     gene            complement(1843..>2906)
                     /locus_tag="VNN25_13990"
     CDS             complement(1843..>2906)
                     /locus_tag="VNN25_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXH92688.1"
                     /translation="WRTIASNPFKVELNRAIQGLYSPGSVFKAVMAVAGLSEGAIDTS
                     TSFFCSGSGIFFGRRFRCDARNGHGDISVANALKVSCDIFFFNIGARLGVDRIAEYAH
                     KLTFGKISRIDLDGEKPGLVPSTQWATTKQHRKWYPSETISVAIGQGPLLVTPLQVAN
                     FMAAIANGGTVYRPHVVRMIEKVDLDGHVERLQVGSEVLHKIALAPKALETVKLGLWK
                     VVNEEGGTGSNARVEGLNVSGKTGTVQVIAQHTWGMTEGLPYKYKDHAWFASYAPNEN
                     PQMVVVVFIEHGGHGSVDAAPLAKLLYESRFPAQVRSANLDLSSPDTLRAIKEGRSPI
                     PGQKKKVAPSLGSNPNLGH"
BASE COUNT          538 a          837 c          936 g          595 t
ORIGIN      
        1 cgagaaactc ggcgccggcg atgcgcttcg cttcttcctc gaaaacagcg atgaattcgt
       61 tcccgatctt ctttcgcttg gcctcggggt cttcgacgcc ggcgagcttg tcgtagaagc
      121 gctgtcttcc gtcgatggcg atgatgtgca tgccgaggcc ctcggacagg cgcgacatga
      181 cctggatcgc ttcatttttt cgcaagacac cgttgtcgac gaagatcgcc gtgagctgat
      241 cgccgatcgc ctcggcgacc agcaacgcgg ccacggatga atcgacgccg ccgctgaccg
      301 cagcgatgac ctgtttgtcg ccgaccgtcg cgcggatttc ctcgaccttt cgcttgcggt
      361 agtcaccgag gttccaggtg ggagtgcagt gggcgatctc gaaaaggaag ttgcgaagga
      421 tcgacgttcc cgcttcgctg tgcgtgacct caggatgaaa ctggattccg tagcaccgcc
      481 gttcgatgtt ctcgaaggcg gcgatcggcg cgttcgccga cgaggccgtc gtgcggaagc
      541 cgggaggctc gcgcaggatg ttgtcgccat ggcttgccca cacgcgctgg tgcgctggcg
      601 tacccgcgaa gagcggcgag tcgttggcgc ggacatcgat ctccgcgcgg ccgtattcgc
      661 gctcggccga cgcttcgact ttcccatcga gcaggtaggc cgtgagctgc atgccgtagc
      721 agatgccgag gatcgggacg ccgaggtcga agatctcgcg cgaacagatc ggcgcggcat
      781 cggcgtaaac cgattgcgga ccaccggaaa agatgatggc ttgcggcgca agtgcgcgca
      841 cccgatcgat cgaaatgtcg aacggttgga cgacggagta aacgctctgc tcgcggatac
      901 ggcgggcgat gagctgcgtg tactgcgaac cgaaatcgag aacgagtacg gtctggccgt
      961 tcgcggcggg atgccgattc tgcccggacg acactacaga aggttccgca gttctttgcc
     1021 gggcttgaag cggatcgtgt tgccgggtgg gatcttcacc tcgacgccgg ttttcggatt
     1081 ccggccgatc cctttcttcc gttgtttgac ctggaaaacg ccgaatccgc ggagctcgat
     1141 ccgctgcccg cgcgcgagcg cgttgcgcag cccttcgatc acggtgtcga ccgcctgagc
     1201 ggccttcacg cgcgaaattt cggacacttc cgccaccttg ttgacgatgt cggctttaat
     1261 catgggcggg attataacct cttccgaatg agcgactcag agcgtcatcg catcgacacc
     1321 tggctcaagc atgtctgcct cgtcaagcat cggaccgatg cgacggaggc ctgccgcggt
     1381 ggtcacgtga aactcaacgg gcagcgggtc aagccggcgg ctccggtgcg cgagggcgac
     1441 gtcgtcgagt tgtatgccgg cgagcgcttc cggaagtttg tcgtgaaggg tatcccggac
     1501 aggcaacagg cgaaagagat cgcccggacg atgtatatcg atgagacgcc ggtcatcgag
     1561 aaggtcgacg attcggtgtt ccgcgagcgg ggagcgggac gtccgacgaa gcgcgagcgc
     1621 cgagacgcgg agaagttcaa gaggaactac tgacgcgatt gccttcggcg ttgggtgaat
     1681 tgcctgcggc gttgtcggtt gtcggttgat tgccttcggc gtgtcgcgga gagaagttca
     1741 ttatgttcgt cctatgcgtg ttgtaggccg ttgtaacgcc gaaggcaact aactgacaac
     1801 cgacaacgcc gaaggcaatt cacgccaacg ccgcaggcaa aatcaatggc cgaggttcgg
     1861 gttggaaccc agcgatggcg caaccttctt tttctgcccg ggaattggcg accggccttc
     1921 tttgatggct cgcagagtgt cggggctgga gagatccagg ttggcgctgc gcacctgtgc
     1981 ggggaatcgc gattcataga gcaacttggc gaggggcgcg gcgtcgacgc tgccgtgacc
     2041 tccgtgctcg atgaaaacca ccacaaccat ctgcgggttt tcattcggcg catacgaagc
     2101 gaaccacgcg tgatctttgt atttgtaggg cagcccttcc gtcatccccc aagtgtgctg
     2161 ggcgatgact tgcaccgttc cggtcttgcc ggaaacgttc agaccttcga cgcgagcgtt
     2221 gctgccggtg ccgccttctt cattgacgac tttccagaga cctagcttga cggtctcgag
     2281 tgcttttgga gcgagtgcga tcttatgcag cacctcgctc ccgacttgca gccgttcgac
     2341 gtgaccgtcg agatcgacct tctcgatcat gcgcacgacg tgcgggcgat aaacggttcc
     2401 gccgttggcg atggctgcca tgaagttcgc gacctgaagc ggcgtgacga gaagtggtcc
     2461 ttgaccgatg gcgaccgaga tcgtctccga tggataccac ttgcgatgct gctttgtggt
     2521 tgcccactgc gtcgaaggaa cgaggccggg cttttcgcca tcgaggtcga tccgcgagat
     2581 cttgccgaac gtcagcttgt gcgcgtattc ggcgatgcga tcgacgccga gccgcgcgcc
     2641 gatgttgaag aagaagatgt cgcatgagac tttcagtgca ttcgcgacgc tgatgtcacc
     2701 gtggccgttc ctggcgtcgc aacggaaacg gcgtccgaaa aagatgccgc tgccgctgca
     2761 gaagaacgag gtgctcgtgt cgatggcacc ttcggacaga ccggctactg ccatgaccgc
     2821 cttgaagacg gatcccggtg aatagagacc ctggatggcg cgattcagct cgaccttgaa
     2881 cggattcgac gcgatcgttc gccaga
//
LOCUS       DATLVW010000461         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_269543, whole genome shotgun sequence.
ACCESSION   DATLVW010000461 DATLVW010000000
VERSION     DATLVW010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269543"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VNN25_13995"
     CDS             complement(<1..785)
                     /locus_tag="VNN25_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH92689.1"
                     /translation="MDRLALLQNAKRVGYVFSGGSSRCAFQIGVIEALAELGVIPALA
                     IGVSAGAWNAAMVAARSEKRIRYYWRSFMRMPHIDLRNLLVDHSPWRFRRLHERTFGR
                     FMGRDRFKQPDALPLLVSVTRFRDGANVIFDARDVDDPLQLLLATNYFPPFYTHRPRI
                     AGEAYGDGGLSDNAPYEKALELGCDAVVVVTQKGESEGGLFKAKGDYDHVVSDPRVVV
                     IRPRHRLPFRFTERRWHVLDVLARIGYLRTREVLLGEEHEETNV"
     gene            809..1405
                     /locus_tag="VNN25_14000"
     CDS             809..1405
                     /locus_tag="VNN25_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92690.1"
                     /translation="MNIKRCVVFFLALLTITCDSGTKSGPHVAKEPISVRGWITDVDA
                     GENAAAYHTIETESARKASLFHDTNVWVENAPYVSGGVAETGAFLLLDVPPGNVTITF
                     TAPNAPAAHLVLAGVPGNADVFVPSMLLKKGSVVLLDPKSVKVRMAMQIEKEVPSGKF
                     VTIAGQSIPIMNTPIAQMSDRHDYPMAPATYQPLAKVK"
     gene            complement(1568..2167)
                     /locus_tag="VNN25_14005"
     CDS             complement(1568..2167)
                     /locus_tag="VNN25_14005"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HXH92691.1"
                     /translation="MVRHLFRCVAFLLCSLPALAATNTRDFNVYLGDASSETNRHGQS
                     RFRTISFELTTGPHYFKRYAERWLPNARVGVDIDYADVRQPRSWFGHTYGDPDDSVRA
                     EWAHFFVRQYWLTSSNSIRPYADLGTGPMWSNRRIPAATSKLNFNSQLGLGVVLFPNA
                     RTPVYFGYRFFHISNGGLTGRNPGLNVNGFFIGTSLRRR"
     gene            2189..>2662
                     /locus_tag="VNN25_14010"
     CDS             2189..>2662
                     /locus_tag="VNN25_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HXH92692.1"
                     /translation="MAPRVNWLGSKTMADRRQHVHRNPLSRFASAATAWTGSTPAFVS
                     AAAIILIWIVTGPLFHFSDTWQLVINTGTTIVTFLMVFLIQNTQNRDTRALHLKLDEL
                     LRALPEAREHEFMDLEDRDEGELKAERVKLFEECGVCRTPEEHAKHAVEIKTQARH"
BASE COUNT          556 a          810 c          835 g          461 t
ORIGIN      
        1 acattcgttt cctcatgctc ctcgccgagc agaacttcgc gcgtgcgcag gtaaccgatc
       61 cgcgccagaa cgtcgaggac gtgccagcgg cgctcggtga atcggaaggg aaggcggtgg
      121 cgcggacgga tgacgacgac gcgcggatcg ctgaccacgt ggtcgtagtc gcctttggct
      181 ttgaagaggc caccctccga ttcgcctttc tgggtcacga cgacgacggc atcgcatccc
      241 aactccaacg ccttctcgta cggcgcgttg tcggagaggc caccgtcgcc gtaggcctcg
      301 ccggcgatgc gcggccggtg cgtgtagaac ggcggaaagt agttcgtggc cagaaggagc
      361 tggagcggat cgtcgacatc gcgggcatcg aagatgacgt tggcgccgtc gcggaagcgg
      421 gtcaccgaaa cgagcagagg cagcgcgtcg ggttgtttga atcgatcgcg gcccatgaac
      481 cggccgaagg ttcgctcgtg caaacgccgg aagcgccacg gcgaatgatc gacgaggagg
      541 ttgcgcaggt cgatgtgcgg catgcgcatg aatgagcgcc agtagtagcg gatgcgcttc
      601 tcggagcgcg ctgcgaccat cgctgcgttc caggcacccg ccgaaacgcc gatcgccagc
      661 gccggtatca caccgagctc ggcgagcgct tcgatgacgc cgatctgaaa ggcacatcgc
      721 gaggagccgc ccgaaaaaac atacccaacg cgttttgcgt tttgcagcag agcgagacga
      781 tccaaggaac tcagtctatg cttttgcggt gaacataaaa cgatgcgtcg ttttctttct
      841 cgcgctcctc accatcacct gtgattccgg gacgaaatcg gggccacacg tcgccaagga
      901 gccgatcagc gtccggggct ggatcaccga cgtcgatgcc ggagagaacg cggctgcgta
      961 ccacacgatc gaaacggagt cggcgcgcaa agcgagtctc ttccacgata cgaatgtctg
     1021 ggtcgagaac gcgccctatg tctccggagg ggtcgccgaa accggcgcat ttctcctgct
     1081 cgatgtgccg cccggaaacg tgacgatcac cttcacggcc ccgaacgccc ccgccgccca
     1141 cctcgtcctt gccggcgtcc ccggcaacgc cgatgtcttc gtaccgtcaa tgctgctgaa
     1201 gaaaggatcg gtcgtcctcc tcgatccgaa gagcgtgaag gtgcgcatgg caatgcagat
     1261 cgagaaagag gtgccgagcg gcaagttcgt gaccatcgcc ggccagagca tccccatcat
     1321 gaacacgccg atcgcgcaaa tgtccgatcg ccacgactac ccgatggcgc cggcgacgta
     1381 tcaaccgttg gcgaaggtga agtagggctt ttcgatggtg gctgggcccc tcatccggcc
     1441 ttcggccacc ttctccccat ctgagaagcg atggggagaa ggggctcgat tggagagttg
     1501 taatttaatg gtcggtcttt tctcgaatcc tctcgcccaa ttcaaccctt gagcgccgca
     1561 atgtcgttca ccgccgccgc aaactggtcc cgatgaagaa accgttcacg ttgagaccgg
     1621 ggttgcgtcc ggtcaggccg ccgttcgaga tgtggaagaa gcgatagccg aagtacaccg
     1681 gcgtccgcgc attcggaaaa aggacgacgc ccaatccgag ctgcgaattg aaatttagct
     1741 tcgaggtcgc ggccgggatg cggcgattcg accacatcgg tcccgtaccg aggtcggcat
     1801 acggccggat cgaattggat gacgtgagcc agtactgccg cacgaagaag tgcgcccact
     1861 cggcgcgcac ggaatcgtcg ggatcgccgt aggtgtgacc aaaccaactg cgcggctgtc
     1921 gaacatcggc gtaatcgata tcgacgccga cacgggcgtt gggcagccag cgctcggcgt
     1981 agcgtttgaa atagtgaggt ccggtcgtca actcgaagct gatcgtgcgg aagcgggact
     2041 ggccgtggcg gttcgtttcg ctgctggcgt cgccaaggta gacgttgaag tcgcgagtat
     2101 tcgtggcagc gagcgcaggc agcgaacaga gaaggaacgc aacacatcga aaaaggtggc
     2161 ggaccatggc cgcgcattct aaacggcgtt ggcaccgcgc gtgaactggc tcggtagtaa
     2221 aaccatggcc gaccgaagac agcatgtcca ccgcaatccc ctttcccggt tcgccagcgc
     2281 agctaccgcg tggaccggct cgacgccggc gttcgtgagc gcggcggcga tcatcctcat
     2341 ctggatcgtc accggtccgc tctttcactt ttccgatacc tggcaactgg tcatcaacac
     2401 gggcacgacg atcgtgacct tcctgatggt ctttctcatc cagaacaccc agaaccgcga
     2461 cacccgggca ctgcatctca aactcgacga gctgctgcgc gcgctgcccg aggcgcgcga
     2521 gcacgagttc atggacctgg aagaccgcga cgaaggcgag ctcaaagccg aacgcgtgaa
     2581 gttattcgaa gagtgcgggg tgtgccggac cccagaggaa cacgcgaagc acgctgtcga
     2641 aatcaagacg caagcccggc at
//
LOCUS       DATLVW010000462         3528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_390654, whole genome shotgun sequence.
ACCESSION   DATLVW010000462 DATLVW010000000
VERSION     DATLVW010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390654"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..2849
                     /locus_tag="VNN25_14015"
     CDS             327..2849
                     /locus_tag="VNN25_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92693.1"
                     /translation="MRKLPFLLLVFSLSAFAEVQPYLVRDLPGYTVHDSMPFGRTFWT
                     TIGNMTWFIANTTGKSIEVFKSDGTTAGTVQVTHGNGVPEIQFLGPYLGTLNGRLIYG
                     GTDTGGEGVFALDTSGGEPVLLGRFVLRYLTNGVVRGDALYFFGPTTSERELWRTDGT
                     PAGTSKIDLMPGNQGAFDPDRDPSLFALGPWIFFYGATPQGTGLHRTDGTIANTTLLL
                     PLSASVLFNDTSEIIPLGDRMLFSLRGAQGNNALWATDGTPAGTSQIATVRAFSPIGI
                     ISGKLFFDGGGLWTTDGTAAGTRVTDVNVSTTSYSGRVLGSRLFLFGQVNDAGKLVST
                     LYVSEGTVATTRPIMKVDQHGYTSLGEGFVIGSNFYFRHDDGIHGMELWRTDGTVTEM
                     LADINPGYRTGIEDVYGEERPDGKVIFVAKELTTGREPWITDGTAAGTHLLANCAPED
                     SLNGSSPRQLRASGDSVFFVATLFGGDAIGVSDGTSAGTAATLVNSPWTISSPAAANG
                     RYFFSTAPTNSPGQIYATDGTAAGTTPIFEQSASPHAMANGILFAAGRDLWFSDGTIA
                     ATRKIHTFDASSFLPRFFSAGEVEWITQGPRLWTTDGTEAGTVELIPSQPPTGEVYEV
                     IRAGKLIYFIESSTFDHGARLWRSDGTAAGSTIVKDLAAQTVTEFVGAAGPFVYFALN
                     GTLYRSDGTANGTIALPVSSPCIFSGAAVGDALALTSFASNGTMTVWRSDGTSAGTAQ
                     LAAMLTKDGSSGCKSLTAHGNAAYFSGWDAVHGWELWTTDGTSSGTKMLADIYPGTKS
                     SLPGELTLAGDRIFFSADSPSIGRELWAVGGHSIPRRHASHP"
     gene            complement(2948..>3528)
                     /locus_tag="VNN25_14020"
     CDS             complement(2948..>3528)
                     /locus_tag="VNN25_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXH92694.1"
                     /translation="VKPANGGSSVGVTKVKNDADLSAAIEKALRFDEKALVERGIDAA
                     EIEVAVLGNDDPQASVPGEIIAGREFYDYEDKYVENNTQLIIPAKLPKEKTDEICRMA
                     ITAFKAIGAAGFARVDFFLERGKNRLYVNEINTIPGFTKISMYPKLWEATELKYPRLI
                     ERLIELGIERSRSRAARYESTMKWFDEVKGIV"
BASE COUNT          675 a         1087 c         1066 g          700 t
ORIGIN      
        1 gtcccgagtc ggccgatctc attcctcatg tcgacgccat gagaaaggcc ctcggcaagc
       61 gcattcgtaa gtccaagtcc aagccggccc cgaagccggc gaacccggtt acagcggctc
      121 ccgaggattc ctcgccggcg acgccatcgt catcgacgac cgatccgaaa actgagtaac
      181 cggtaacgca gtaagcagta agtcccgcgc gggcgcgtcc gaaggcgcgc ccgcgcattt
      241 gtttttcgga aatcgactga tggctgcgcg gcagttccgg gtgtcatgca acctcacccc
      301 gctgggatag aatctcgggt tctcccttgc gcaaactccc gttcctcctt cttgtcttct
      361 ccctctccgc gttcgctgaa gtgcagccct acctcgttcg cgatcttccg ggatataccg
      421 ttcatgactc gatgccgttc ggacgcacgt tctggacgac gatcggcaac atgacctggt
      481 tcatagcgaa caccacaggg aaatccattg aagtattcaa gagcgacggg acgacggccg
      541 gcacggtaca ggtcacacat ggcaacggcg ttcctgagat tcagtttctc gggccgtatt
      601 tagggacact caacggaagg ctcatctacg gcggcacaga taccgggggt gaaggcgtct
      661 tcgcgctcga cacgagtggc ggcgagccgg tgctcctcgg ccgcttcgtg ctgcggtatc
      721 tcaccaatgg tgtcgttcgc ggcgatgcgc tttatttctt cggaccgaca acatcggaac
      781 gtgagttgtg gcgtacggac ggcacacctg ctggcacatc gaagatcgat ctgatgcccg
      841 gcaaccaggg cgcattcgat ccggaccgcg atcccagcct cttcgcactc ggtccgtgga
      901 ttttcttcta tggagccacg ccgcagggaa ccggcctaca tcgcacggac ggcacgattg
      961 cgaacacgac gctgctgctg ccgctttcgg ccagcgtgct cttcaacgac acaagcgaaa
     1021 tcatccccct tggtgatcgc atgctgttct cgctccgagg ggctcaaggg aacaacgcgc
     1081 tctgggcaac cgatggcacg ccggcaggca cgtcacagat cgcaacggtg agagccttct
     1141 ccccgatcgg catcatcagc ggcaaactct tcttcgatgg aggaggtctc tggacgaccg
     1201 atggaacagc agccggaacg cgagttaccg acgtgaacgt cagcacgacc tcctattctg
     1261 gacgcgttct cgggagccgg ctcttcctct tcggccaggt taacgatgct ggcaaactgg
     1321 tttccacgct gtacgtgagc gagggaaccg tggccacgac gcggccgatc atgaaggtcg
     1381 atcaacacgg gtatacgtcc ctgggcgaag gattcgtcat cggcagcaac ttctattttc
     1441 gccatgacga cggtattcac gggatggagt tgtggcggac cgatggaacg gtcaccgaga
     1501 tgctcgccga catcaacccg ggatatcgca ctggaatcga agacgtgtat ggcgaggagc
     1561 ggccggacgg gaaggtgatc ttcgtcgcca aggagctcac tacgggccgc gagccttgga
     1621 tcacggacgg cacggcggct ggaacgcacc tcctcgcgaa ctgcgcgccg gaggattcgc
     1681 tcaatggatc atcaccacgg cagctgcgcg cgagcggtga ttcggtgttc ttcgttgcga
     1741 cgctctttgg aggtgatgcg atcggtgtct cggacggcac gagcgccggg accgcagcaa
     1801 cgctcgttaa ctctccgtgg acgatctcga gccccgcggc ggcgaacggc cgctacttct
     1861 tctcgaccgc acccacgaat tctccaggac agatctacgc caccgacggc accgccgccg
     1921 ggacgactcc gatcttcgag caatcagcct ctccgcatgc aatggcaaac ggcatcctct
     1981 ttgcggcagg acgggatctc tggttctccg acggtacgat cgctgccacg cgaaagatcc
     2041 acacgttcga cgcaagttct ttcctgccga ggttcttttc tgcaggtgag gtcgaatgga
     2101 tcacgcaagg tccgaggctt tggacgacgg acggaaccga agcgggaacc gtcgagctca
     2161 ttccgagcca gccgccgact ggcgaggtgt acgaggtgat ccgcgccggc aaactgattt
     2221 acttcatcga atcgagcaca ttcgatcacg gcgcacggct atggcgctcg gacgggacgg
     2281 cagcaggatc tacgatcgtc aaagatctgg ccgcgcagac agtgacggag ttcgtcggcg
     2341 ccgccggccc attcgtctac ttcgctttga atggaacgct gtatcgcagc gatggaacgg
     2401 ccaacggcac gatcgcgttg ccagtcagct cgccatgcat cttcagcggt gctgccgtcg
     2461 gtgacgcact ggcgctcacg tcgttcgcat ccaacggcac aatgaccgtg tggcgctccg
     2521 acggcacgtc cgcgggaacg gcgcagctcg ccgcaatgct gacgaaagac ggaagttccg
     2581 gatgcaaatc gctcacggca cacggcaacg cggcctactt cagcggctgg gacgcagtcc
     2641 acggctggga gctctggacg accgatggca cctcttcggg cacgaagatg ctggccgata
     2701 tctaccccgg taccaagagc tcattgccgg gagagctgac gctcgcgggt gaccgcatct
     2761 tcttttccgc cgactcgcca agcatcggtc gggagctatg ggcggtaggg ggacattcaa
     2821 tcccgcgtcg tcacgcgtcc catccctgag tggagtgggt gcagtgagcg gcggagcagg
     2881 aatccacgct cactcgtgag gaccgcaggt gaagcgcggt gagacctggt gggcagacat
     2941 ggtagcccta cacgatcccc ttcacctcat cgaaccactt cattgtgctt tcataacgcg
     3001 cggcccgcga tcgggagcgc tcgatgccta gctcgatcag gcgttcgatc aggcgagggt
     3061 atttgagctc cgttgcttcc caaagtttgg ggtacatcga gattttcgtg aagccgggga
     3121 tggtgttgat ctcgttgacg tagaggcggt ttttgccgcg ctcgaggaag aagtcgactc
     3181 tcgcgaagcc tgcggcgccg atcgctttga aggcagtgat ggccatgcgg cagatctcgt
     3241 cggttttttc cttcggcagt tttgcaggaa tgatcagttg cgtgttgttc tcgacgtatt
     3301 tgtcttcgta gtcgtagaac tcgcggccgg cgatgatctc gccgggtacg ctcgcttgcg
     3361 gatcgtcgtt gccgaggacg gcgacttcga tctcggcggc atcgataccg cgctccacca
     3421 gcgccttctc atcgaagcgc aacgccttct cgatggccgc gctcaggtcc gcgtcattct
     3481 tgaccttcgt cacacccact gacgagccgc cgttcgccgg cttcacaa
//
LOCUS       DATLVW010000463         4809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_598701, whole genome shotgun sequence.
ACCESSION   DATLVW010000463 DATLVW010000000
VERSION     DATLVW010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598701"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VNN25_14025"
     CDS             complement(<1..853)
                     /locus_tag="VNN25_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXH92695.1"
                     /translation="MRSIRVLAATALFFVFIVPSFGACTFPVTVSDGLGNVHRLSWQP
                     TAGATNYEVQVSHDGFQHSTTLANLPAGTTGVTVSVLTNSFQPYSYRIIASNPSNASN
                     VSCTGAATQSFADPAFDQRVNRIVIPVAGSTRGANNAQFKTSLRLGPPTSLNIGKIIF
                     HPAGRAGSDDDPSIPYRVDRGDTLQFDDIVAAIGQSGIGSIDITSDSSFGLPERGLLP
                     VEARLFNEGPGGTYGTFEEAVLPPDAYRPIDWNIFVPSTRFRVNLGIRTLTAAHVTFY
                     HFAAGGPV"
     gene            complement(1026..2318)
                     /locus_tag="VNN25_14030"
     CDS             complement(1026..2318)
                     /locus_tag="VNN25_14030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH92696.1"
                     /translation="MSSSLPQEQTVEANAQAGIPGGRFQRTFSALRYRDFRLLWFGAF
                     ASTTGTFMQTLAQGWLVYTMTGSAFLLGVDGFLATGPMLLFSLFGGVIADRIERRKIM
                     LLSQVMQMTFAFVLTGLIYFHAVKIWHIFILSFLTGSAQSFSGPAYASLLPLLVKRDD
                     MSNAVAMNSMQFNLARVIGPAIGGVVFGVWGATACFGINGLSFVAVIIALLIIKMPPL
                     HADATSTSMMAQMKQGFAFVASRRSLLMLTFLSFAGTFLGMPLFTMMPVVAKSIFHLG
                     PQGLSILQADYGVGSVVGALFVAGSSYAAKKGRLALMLQLVFACTLVAFGFSRHITSS
                     LVIAFLAGAAIVGVISLYSSLVQLSTSDAMRGRVMSIFMLAFRGGMPLGNLLAGFVAQ
                     RWSISTALEVNGIALAVVALIFIARGTDLDAGLEHAPV"
     gene            complement(2315..2815)
                     /gene="ruvC"
                     /locus_tag="VNN25_14035"
     CDS             complement(2315..2815)
                     /gene="ruvC"
                     /locus_tag="VNN25_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXH92697.1"
                     /translation="MRILGIDPGSVTTGFGVIEQVRGRLALIEQGTIGTTRGAELADR
                     LCRIHDELVAVIARNALDAVAVEAPFGGQNIKSLIQLAHARGVILLAARAAKLDVFEY
                     SPRSVKSAIVGYGGAEKEQVAKMVRMLLPGCANLVMSTDASDALAVAICHAHTAGTAE
                     RLRIAR"
     gene            complement(3016..4695)
                     /locus_tag="VNN25_14040"
     CDS             complement(3016..4695)
                     /locus_tag="VNN25_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH92698.1"
                     /translation="MKTSLRCIVVVVSLVLLAHAGTGAVLPDKDIRAILVDRIDVQRQ
                     GVGIVVGVIDPSGRRTVAYGNINKGEGPVDADTVFEIGSVTKVFTSLLLADAVRRGEV
                     ALTDPVSKYLPPDVKVPERGGKKITLVDLATHTSGLPRMPTNFHPKDPGNPYADYTEA
                     QLYEFLSSVELTRDIGSHYEYSNLGGGLLGHALARRAGTDYETLVRTRILEPLGMKST
                     AITLSKSMKEHLTPGHDARLRQVPNWDLPTLAGAGALRSTANDLLTFLAANIGIEKSP
                     LAPSMAAMIATRRPTGNPNLEIALGWHIWNRNGHEIIWHNGGTGGYRTWIGFEPKSRT
                     GAVVLSNTTTNAGVDDIGLHLLDPAVPLLPPAKEHHEVKVDAAVLEKYSGRYQLAPNF
                     ILTIMREGDHLYSQATGQPRFEIFAEGGHDFFVKEFDAQLTFVVDANGRATSLVLHQN
                     GDHPAKRLEGEPPPVKEHKEVAVDPKIFDGYVGRYQLAPNFVLAITRDGDRLYSQATG
                     QQRFEIFAEGAHDFFFKVVDAQITFVTDAAGHATSLTLHQNGRDVPGRRVE"
BASE COUNT          960 a         1416 c         1584 g          849 t
ORIGIN      
        1 tcacagggcc cccggccgcg aagtgataga aggtgacgtg cgcggcggtg agagtgcgga
       61 tgccgaggtt gacacggaac cgtgtggagg ggacgaagat gttccagtcg atcggccggt
      121 aggcgtccgg cggcagcacc gcctcttcga acgtgccgta tgttcccccg ggcccttcgt
      181 tgaacagccg tgcttcgacc ggcaacaggc ccctctcggg aagaccgaag gaagagtccg
      241 aagtgatgtc gatggacccg ataccgctct ggccgatcgc ggcaacgatg tcgtcgaact
      301 gcagcgtatc gccgcggtcg acacggtacg gaatcgacgg atcgtcatcg ctgccggcac
      361 gccctgccgg atggaagatg atctttccga tgttcagcga cgtcggcggc ccaaggcgaa
      421 gcgatgtctt gaattgggca ttgttcgcgc cgcgagtcga gccggctacc ggaatgacga
      481 ttctgttgac gcgctgatcg aatgcggggt ccgcaaagga ttgggtcgct gcgccggtac
      541 acgaaacgtt cgatgcgttc gacgggttcg acgcgatgat gcggtaggag tacggttgga
      601 aggaattcgt caggacgctc accgtgacgc cggtcgtacc agcaggaaga ttcgccagcg
      661 tcgtggaatg ctgaaaaccg tcgtgcgaca cctgaacttc ataattcgtc gcgccggcgg
      721 ttggctgcca cgacagccgg tggacgttcc caagcccgtc cgagacggtc accgggaagg
      781 tgcacgcccc gaaggacggc acgatgaaca cgaagaacaa agcagtagct gcgagaactc
      841 ttattgatct catgtacctc tctcctgttt gattgtgcgc ggcggcgtat gaaggtacag
      901 cggcgcgtaa ggatttccgg cgggaaacta cgagatgggc gcgaagctcg gacaacgttg
      961 agcctggata ccgatacgtt agccccactg acgcgccgcg tcaagttgtc cggagagcgt
     1021 ttacgtcaga caggcgcgtg ttccagcccc gcatcgaggt ccgttccccg cgcgatgaag
     1081 ataagggcca cgaccgcgag cgcgatgccg ttcacttcca gtgcggtcga gatcgaccac
     1141 cgctgggcga caaaaccggc caggagattg ccgagcggca tgccgccgcg gaacgccagc
     1201 atgaagatcg acatcacacg tccccgcatg gcgtcgctcg tcgataactg cacgagtgag
     1261 ctgtaaagag agatgacgcc gacgatggcc gcacccgcga ggaaagcgat gaccaggctc
     1321 gacgtgatgt gccgcgaaaa tccgaacgcc acgagcgtgc aggcgaagac gagttggagc
     1381 atcagcgcca gcctcccctt ctttgccgca tagctgctgc cggcgacgaa gagcgcgccg
     1441 acgaccgacc caacgccgta atcggcttgc agaatcgaga gaccttgcgg cccgaggtgg
     1501 aagatgctct tggccacgac cggcatcatc gtgaagagcg gcatgccgag gaatgtgccg
     1561 gcgaacgaga gaaaggtcag catcaacagg gatcgccgcg atgccacgaa cgcgaatccc
     1621 tgtttcatct gcgccatcat cgacgtcgat gtcgcatccg cgtgcagtgg cggcatcttg
     1681 atgatgagca gcgcgatgat cacggcgacg aacgacaagc cgttgatgcc gaaacaggcg
     1741 gtggcacccc acacgccgaa gaccacgccg ccgatcgccg ggccgatgac gcgcgccaga
     1801 ttgaactgca tcgagttcat ggccacggcg ttcgacatgt cgtcgcgctt gaccagcagt
     1861 ggcagcagcg acgcgtacgc cgggccgctg aacgactgcg cgctcccggt cagaaacgag
     1921 agtatgaaga tgtgccagat cttgacggcg tgaaagtaga tgaggcccgt cagcacgaag
     1981 gcgaacgtca tctgcatgac ctgcgagagg agcatgatct tgcgccgctc gatgcggtcg
     2041 gcgatgacgc cgccgaagag cgagaagagc agcatcgggc cggtggccag gaagccgtcg
     2101 acgccgagga ggaaggccga gccggtcatc gtgtagacga gccagccctg cgccagcgtc
     2161 tgcatgaacg tgccggttgt cgacgcgaac gcaccgaacc agagcagtcg aaagtcgcgg
     2221 tagcgcaacg cgctgaacgt gcgttggaac cggccgcctg ggatgcctgc ctgcgcgttc
     2281 gcttcgaccg tctgttcctg cgggagcgac gagctcatcg agcgatccgc aacctctcgg
     2341 cggttccggc ggtgtgggcg tggcagatgg cgacggcgag ggcgtcggag gcgtcggtgg
     2401 acatgacgag attcgcgcaa ccaggaagga gcatgcgaac catcttcgcc acctgttcct
     2461 tctccgcgcc gccatagccg acgatcgcgc tcttgacgct gcgcggcgag tattcgaata
     2521 cgtcaagctt cgccgccctc gccgccagca gaatcacgcc gcgggcgtgc gcaagctgga
     2581 tcagactctt gatgttctgc ccgccgaacg gcgcttcgac ggcgacggcg tcgagcgcat
     2641 tgcgcgcgat gacggcaaca agctcgtcgt ggatgcggca aagccggtcc gccagctcag
     2701 ccccgcgcgt cgtcccgatc gtcccctgct cgatcaacgc cagccgcccc cgaacctgct
     2761 cgatgacgcc aaatcctgtc gtgacggagc caggatcgat accgagaatg cgcacggacg
     2821 gattgtaacg aggatcggtt acagatgact gttgaatcga agacggaact gggtctaaag
     2881 ggtcaggtct aaagtctaaa gtgccgagga caatgttggc gtgaagggtc aggtctaaag
     2941 tctaaagtgc cgaggacgat cgtccctaag acaatgagcc ctattcggca ctttagactt
     3001 tagacctgac ccagatcatt ccacccgcct cccgggcacg tccctcccgt tctggtgcag
     3061 tgtcaggctg gtggcgtgtc ctgctgcgtc ggtgacgaac gtgatctgcg cgtcgacgac
     3121 tttgaagaag aagtcgtggg cgccctcggc gaagatctcg aaccgctgct gcccggtagc
     3181 ttgcgaataa agccggtccc cgtcccgcgt gatcgcaagt acgaagttcg gcgcgagctg
     3241 gtagcggcca acgtatccgt cgaagatctt cggatcgacg gcaacctcct tatgttcttt
     3301 caccggtgga ggctcgcctt ccaaccgctt cgcaggatgg tcgccgttct gatgaagcac
     3361 gagactcgtc gcgcggccgt tggcatcgac gacgaacgtg agctgcgcgt caaactcttt
     3421 gacaaagaag tcgtggccgc cctcggcgaa gatctcgaac cgcggctgac cggtagcttg
     3481 cgaataaagg tggtcgccct cccgcatgat ggtcagaatg aagttcggcg cgagctggta
     3541 gcgtcccgag tatttctcga ggacagcagc gtcaaccttc acctcatgat gctccttcgc
     3601 cggcggcagt agcgggacgg cgggatcgag aagatgcagg ccgatgtcat cgacgccggc
     3661 gttcgtggtc gtgttcgaga gtacgacagc gccggtgcgg ctcttcggtt cgaatccgat
     3721 ccaggtgcgg taaccgccgg tgccgccgtt gtgccagatg atctcgtgtc cgttgcggtt
     3781 ccagatgtgc catccgaggg cgatctcgag gtttgggttg ccggtgggac ggcgcgtggc
     3841 gatcatcgcg gccatcgatg gggccagcgg tgacttctcg atgccgatgt ttgcggcgag
     3901 gaaggtcagc aggtcgttcg cggtcgatcg cagcgcgccg gcgccggcca gagtcgggag
     3961 gtcccaattc ggtacctgac gaagccgggc gtcgtgcccg ggagtgagat gctccttcat
     4021 cgacttcgat agtgtgatgg cggtgctctt catcccaagc ggctccagaa tccgggtccg
     4081 caccagcgtc tcgtagtcgg tcccggcgcg gcgggccaga gcgtggccga gaaggccgcc
     4141 gccaaggttc gagtactcgt agtgcgagcc gatgtcgcgc gtcagctcga cggaggacag
     4201 gaactcgtag agctgcgcct cggtgtagtc ggcgtacgga ttgccgggat ctttcggatg
     4261 aaagttcgtc ggcatgcgag gcagaccgga agtgtgcgtg gccagatcga cgagggtgat
     4321 cttctttccg ccgcgctcgg gcaccttcac gtccggcggg agatacttcg aaacgggatc
     4381 cgtgagagcc acttcgccac gccggactgc atcagcgagg agcagcgacg tgaagacctt
     4441 cgtcacggag ccgatctcga agacggtatc ggcgtcgaca ggtccttctc ctttattgat
     4501 gtttccgtac gcgaccgtgc gccggccgga tggatcgatg acgccgacga cgatgccgac
     4561 gccctgccgc tgcacgtcga tgcggtcaac gaggatggcg cggatgtcct tgtccggcaa
     4621 caccgcaccg gtgccggcat gggcgagaag aacgagactg acgacgacaa cgatgcatct
     4681 gagcgatgtt ttcaatgttc ctcctgaaga cgagagagtg cgtcgatctg tcgctgatag
     4741 cggcgagcca tccataggtt gagcggaatc gccgcgatgg ccaggacaac gaatgcgatg
     4801 gtttcataa
//
LOCUS       DATLVW010000464         4178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_310315, whole genome shotgun sequence.
ACCESSION   DATLVW010000464 DATLVW010000000
VERSION     DATLVW010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310315"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2693)
                     /locus_tag="VNN25_14045"
     CDS             complement(<1..2693)
                     /locus_tag="VNN25_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH92699.1"
                     /translation="MTPDSTLHSALLHLPIVTTILSVLFATILFTRYRRHGGGLHLLW
                     WGIGMVTYGIGTMTEASTTLFGWNPIIFKFWYVAGAFLGGYPLAQGSIYLLMSPRFAR
                     WSARIACAVIAVAAILVFLSPLNAALAEPHRLSGRVLEWQWLRAISPLLNLYSVTFLV
                     GGAAVSAIRYRRNPAASNRYAGNIAIAIGGLLPAIGGTMTRYGVVEALYITELAGLLL
                     IYAGYRWCTAAPSRAIAPQRKAAMTAAAMLLVLFPMSAIADDAKPAKPEQPATVAAAT
                     PVTATTDTKAAAKAETPVPSFFAETTVTATGTKRDVFELATPVTVIRQESIEQKSPQN
                     AAELLREQPGVDINGVGPNQGRPVIRGQRGLRVLFLENGLRLNNPRRQTDFGEMTGLV
                     DLNSVSTIEVVRGPASVLYGTDAIGGVLNLISKEPAFNSGSNVGGFVEGRYSNSGKLA
                     GGSAGFDVRFGALTLQLGGTKREVNDYSVPSGQFGNIHLPNKTDVLDTGLHDTTVWGS
                     AGYAASDRDKFNFRFNHYRAGQTGFGYVPANLYGATEEAKIRILYPEQAFDRYLLSYA
                     GTPQNQTWADSTNVQVYYQNNKRQLANDIDINIGPVAPGFPNSSVLANTLNHSDVRTF
                     GLRTDAVKVFGNGRHIITYGFEGTRDHSDNTDYSVTTAIIRTPYGNSTSVAPPDTLAN
                     APNATNLSYGVFGQDEVSVTNKLHVTAGLRYHDVKTDAIATPGWNVNGLGFSDHNIVG
                     ALTATYQITDYMNLLSSYGRGFRSPNIIERLFNGLTPEGSGYQLLNPALKSETSNNFD
                     LGVKYRRWDAFMEAVAFRNDISGGIIQAYLSPSEIAELDAATRAAIKAGGVQFVVQDR
                     NAERLRYQGVEMALGWHSHNGLTLGGNYTWVDGNRTDSINPP"
     gene            complement(2757..3236)
                     /locus_tag="VNN25_14050"
     CDS             complement(2757..3236)
                     /locus_tag="VNN25_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92700.1"
                     /translation="MTTGGGARFKRLRTIALAAPVVLIVALHAVMLLRRVGDASITRP
                     VVLAQWAFAAALLAAALFVRRALSRNGGRRAAIIFWLFVAILHLAPAGERYFDLRQDA
                     ALLVEVGLVAVPAIVILASRQRSDSIQTQSGWIGIDLLAFAPFSSSVCLGDRAPPRF"
     gene            3623..>4178
                     /locus_tag="VNN25_14055"
     CDS             3623..>4178
                     /locus_tag="VNN25_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH92701.1"
                     /translation="MNTPYNVDTQNSCQSCSLRTERQFCNMGTATVKRMDEIKFTAVY
                     PKGSVLFVEGEQPRGVFIICSGRAKLTTSSAEGKTLIVKIAEAGEVLGASAGILGTPY
                     EVSAEMLEPSQVNFIRREDFMAFLNSHPEACLHTAQQLSAKYHSAQREIRALGLSQST
                     SEKLARLFLDWSDRNGEATAKGSRL"
BASE COUNT          821 a         1231 c         1356 g          770 t
ORIGIN      
        1 ggcggattga tcgagtcggt gcggttgccg tcgacccagg tgtagttgcc gccgagggtg
       61 aggccgttgt gcgagtgcca cccgagagcc atttcgacac cttgataacg aagccgctcg
      121 gcgttgcggt cctgcacgac gaactgcacg cctccggctt tgatcgcagc gcgcgtggcc
      181 gcgtcgagct ccgcaatctc cgacggcgaa agataggctt gaatgattcc gccgctgatg
      241 tcattgcgga acgcgacggc ttccatgaac gcatcccagc ggcggtattt gacgccgaga
      301 tcgaagttgt tgctcgtctc ggacttgaga gccgggttca gcaactgata cccgctcccc
      361 tcgggcgtca aaccattgaa cagccgctcg atgatgttgg gcgagcggaa accgcggcca
      421 tacgacgaga gaaggttcat gtagtcggtg atctggtagg tcgccgttag cgcgccgacg
      481 atgttgtgat ccgagaagcc gaggccgttc acgttccagc cgggagtggc aatggcgtcg
      541 gttttcacgt cgtgatagcg aagtccggcc gtcacgtgaa gcttgttggt cacggacact
      601 tcgtcctgtc cgaagacgcc gtagctgagg ttcgtcgcat tcggcgcatt ggcgagcgtg
      661 tccggaggcg cgaccgacgt gctgttgcca tacggcgtgc ggatgatggc cgtggtcacg
      721 gagtagtcgg tgttgtcgct gtgatcgcgc gttccttcga agccgtaggt gatgatgtga
      781 cggccattgc cgaagacttt gacggcgtcg gtacgaagtc cgaacgtacg aacgtcggaa
      841 tggttcagcg tgttggcgag gactgacgaa ttcgggaagc cgggtgcgac gggaccgatg
      901 ttgatgtcga tgtcgtttgc caactggcgc ttgttgttct ggtagtaaac ctgaacgttg
      961 gtggagtcgg cccaggtctg gttctgcggc gtaccggcgt aggacaacag gtatcgatcg
     1021 aatgcctgct cgggatagag gatgcggatc ttggcctctt cggtggcgcc gtagagattc
     1081 gcaggcacgt agccgaatcc ggtctggccg gcgcggtagt gattgaagcg gaagttgaac
     1141 ttatcgcgat cactcgcggc gtaaccggcg cttccccaga cggtcgtatc gtgcaggccg
     1201 gtgtcgagga cgtccgtttt gttcggaaga tgaatgttgc cgaactgtcc ggagggaacg
     1261 gagtagtcgt tcacctcgcg cttcgtgccg ccgagttgga gggtcagcgc accgaaccgc
     1321 acgtcgaatc cggcgctgcc gccggcgagc ttgccgctgt tcgagtaacg cccttcgacg
     1381 aagccgccca cgttcgagcc gctgttgaac gcgggctctt tgctgatgag attgagcacg
     1441 ccgccgatcg catcggttcc gtagagaact gaggcggggc cacgaacgac ttcgattgtg
     1501 gagacactgt tgagatcgac gagaccggtc atctcgccga agtcggtctg acggcgcggg
     1561 ttgttgaggc gcagcccgtt ctcgaggaat agcacgcgca ggccgcgctg gccgcgaatg
     1621 accggacgtc cctgattcgg cccgacgccg ttgatgtcga ctccgggctg ctcgcgcaga
     1681 agttcggcgg cgttctgcgg cgatttctgt tcgatcgact cctgacgaat gacggtcacg
     1741 ggcgttgcca gctcgaagac gtcgcgcttc gttccggtgg cggtaacggt cgtttccgcg
     1801 aagaacgagg ggaccggcgt ttcggctttt gcagcggcct tggtatctgt ggttgctgtg
     1861 accggagtag ccgcggcgac cgtcgcgggc tgctccggct tcgccggctt ggcgtcatcg
     1921 gcaatcgcgc tcatcgggaa gagaaccagc agcatcgccg ctgcggtcat cgctgctttg
     1981 cgctgcggtg cgatcgcacg cgacggcgcg gcggtgcacc agcggtagcc tgcgtaaatc
     2041 aaaaggagtc cggccagttc ggtgatgtag agcgcttcca cgacaccgta acgggtcatc
     2101 gtgccgccga tggccggaag aagtccgcca atggcgatgg cgatgtttcc cgcataccga
     2161 ttcgacgctg ctggattgcg gcgatagcga atcgcagaaa ctgccgcgcc gccgaccagg
     2221 aaggtcacgg agtagagatt gagcaacggg ctgatggcgc gcagccactg ccattccagg
     2281 acgcggcccg agagacggtg cggttccgcc agcgcagcat tgagcggcga gagaaacacg
     2341 agaatcgctg cgacagcgat gaccgcgcag gcgatccgcg cagaccatcg cgcgaaacga
     2401 ggactcatca atagatagat ggagccctgc gccagcggat agccgcccag aaacgcgccg
     2461 gccacatacc agaacttgaa gatgatcgga ttccacccaa agagcgtcgt cgaggcttcg
     2521 gtcatcgtgc cgatgccgta ggtaaccatt ccgatgcccc accagagcag gtgaaggccg
     2581 ccgccgtgac ggcgatagcg agtgaagaga atcgttgcaa acagaacgga caggatcgtg
     2641 gtcacgatcg gcaggtgcag cagggccgag tgcagggtgc tatcgggagt catttccaaa
     2701 tcctcatttc gtggtccgat gttcaggacc gtcgcgcggt tgcggatcga aaggattcag
     2761 aagcgaggcg gggcgcggtc gccgaggcag accgaggagc tgaagggcgc gaaggcgaga
     2821 aggtcgatgc cgatccagcc cgattgcgtt tggatgctgt cagagcgttg gcgcgacgcg
     2881 agaatcacga tcgcaggaac ggcgacaagt cccacttcga cgagcaaggc tgcatcctgg
     2941 cgaagatcga aatagcgctc gcccgcaggg gccaggtgaa gaatggccac gaagagccag
     3001 aagatgatcg ccgcacgccg tccgccgttg cgggagagcg cgcgccgcac gaacaaggcc
     3061 gcggccagaa gcgccgccgc aaacgcccac tgcgcgagaa ccaccggacg cgtgatcgag
     3121 gcatcgccga cacggcgaag cagcatgacc gcgtgcagcg ccacgatcag aacgaccggc
     3181 gcggcaagcg cgatcgttcg caatcgtttg aacctcgctc cgccgcccgt cgtcatgggc
     3241 gtaaggttag ggcggcgatg ccattcgctc cgtgcaggga gtcacgagcg cttgtgaccg
     3301 gtatcaccgt gtggctgatc tctgcctttt tcatgaagag atcagagatc agagatcaga
     3361 gacaagagac aagagatcag gagccatcag gaacgagcat cccgcgttct ctcgcgtcac
     3421 cgatctctcc gtcactgatc tctcgtctct gatctctcgt ctctgatctc tctatctctg
     3481 atctctctat ctctgatctc tgcctttttc cccacctatc catgttcgcc tccccacccc
     3541 ccggggtggg gcgctgatca caaatcgagg tgatattggc tacatacgac ggtgatcaca
     3601 ccgaaacgac acggaggcaa cgatgaacac cccttacaac gtcgacaccc agaacagctg
     3661 ccaatcatgc tcgctccgca ccgagcgcca gttctgcaac atgggaacgg ccaccgtgaa
     3721 gcggatggat gagatcaagt tcacggcggt ctatccgaag ggctcggtcc tgttcgtcga
     3781 aggggagcag cctcgcggcg tgttcatcat ctgcagcggg cgcgcgaaac tgacgacgtc
     3841 ctctgccgaa gggaagacgc tcatcgtcaa gatcgccgaa gccggcgaag ttctcggcgc
     3901 cagcgccggc atcctcggca cgccctacga agtctccgcc gaaatgctgg agccgtcgca
     3961 ggtcaacttc atccgccgcg aagacttcat ggccttcctg aacagccacc ctgaagcatg
     4021 tcttcacacc gcgcagcaac tgagcgcgaa gtaccactcc gcgcagcgcg agatccgcgc
     4081 cctcggcctg tcgcagagca cgtccgagaa gctggcgcgt ctcttcctcg actggtccga
     4141 ccgcaacggc gaagcgaccg ccaaaggcag ccgcctga
//
LOCUS       DATLVW010000465         5281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_102762, whole genome shotgun sequence.
ACCESSION   DATLVW010000465 DATLVW010000000
VERSION     DATLVW010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102762"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VNN25_14060"
     CDS             <1..224
                     /locus_tag="VNN25_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92702.1"
                     /translation="TLVGWSVRGFDSFGDDVQRVVKRIVPRVTSGSIVVMHQGRGFSV
                     ACVSSVVRALQAKGYAFVVPLPDRLKTKR"
     gene            complement(176..820)
                     /locus_tag="VNN25_14065"
     CDS             complement(176..820)
                     /locus_tag="VNN25_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH92703.1"
                     /translation="MSRAGLADRVAARYPTRFLRIYVRAKIRMDPVYDAVFDRLGISG
                     QPLLDVGCGVGILPSYLRERGFTAPICGLDHDERKIGIARQVVPDATFEAGDARVAMD
                     RGGTVVLLDLLHYFTSEEQSRILAAAAAHATTVIIRDAVRDGSWRYRMTYAQEMFSRL
                     VRWLRAERLHFATRDEVLRPFAGFEAEVVPLYGRTPFNNYLFVFKRSGRGTTNA"
     gene            complement(841..1257)
                     /locus_tag="VNN25_14070"
     CDS             complement(841..1257)
                     /locus_tag="VNN25_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH92704.1"
                     /translation="MSEVQHHDPDDEKRVADVDRKRVLVIDDDLPLRGMLAAALRQAG
                     FDVLLACDGDEGYRALTLHQPDVILLDLAMPGTNGWDFLQRLRETGFLSTARIIVLSA
                     HLYMEPKAILQLGVSAILPKPFNLPELIDLIEHLSP"
     gene            complement(1454..2455)
                     /locus_tag="VNN25_14075"
     CDS             complement(1454..2455)
                     /locus_tag="VNN25_14075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92705.1"
                     /translation="MPVDARFTIRAFREGDEEQILALFARAFPHAARGREHFQWEYQR
                     SPFGNERISLTFDEAQRLVAQYAGYPVPLVDDGRALAANQIGDIMTDRSVRHIGRGPT
                     SILARTASHFYDTFCRGRVAFNYGFTTAAHRELSVRFLGSDAVEPAPYRVRDLTVNPF
                     PRIGRRERWPRGWQLDLVRGFDSEYDDFFERVAPAYRLLVRRDSRYLRWRYLECPDVP
                     YFVVAIRKWRRLVGWSVFGVRGRRLAWGDALFDPRYPHAVEVLLRHVVPAYPVDRIDG
                     WFPARPAWFDGLLRGLGFESRPDPQDLSLMCVPFDKPEASAQIAERLYYTYGDSDLF"
     gene            complement(2445..3941)
                     /locus_tag="VNN25_14080"
     CDS             complement(2445..3941)
                     /locus_tag="VNN25_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HXH92706.1"
                     /translation="MSSTRVSVLVPLVGPSAELPSTLEAIERYLQATGFEFDIRVLDR
                     RDGAGYGAMLRRGVAEAGGSIIVVADHDLPYPMSAIGDAVALIQSGAADVVFGRRETR
                     PGDLLLRTLLVPILPDRALHLKAFSADAARLIVGETRLRKGGFDLEAAYLANKYGFRV
                     ECLTIHGATSGSKATFDLWSGVAAAVAIRMTDRRNGYRPPRRCLVCFSNEVWSIDQIP
                     GNVVRSCSRCKCRYLGHFDVVEDGGPVRRVLRGQPAPADPGDETLHRGVAREKTSQRR
                     LAALRKHLISRARILEVGVRDGSFGSAAAAEFEYVGIDRAPSVARAVRARGLEVYCST
                     VPNFVNTGPAFDAIALFHVFENMADPHDALSRMKDLLKPGGVLLLSTFDTEGLLYLIT
                     ERRWMTQNFRTHLILYSRSALIELLEHSGFEIVSIGAELEYRDQRLLRHSVATRWPAL
                     MPVMRTLFRILPDPLLVPSGSIRIVAKRRVGSPVNFRAIPSVEPTHAR"
     gene            complement(3938..4954)
                     /locus_tag="VNN25_14085"
     CDS             complement(3938..4954)
                     /locus_tag="VNN25_14085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92707.1"
                     /translation="MKRSLLLINYRSASLAIDAIRTARASSSQPLQVVAVDNSTDASE
                     ARALGPVADVVITAPRNLGYAGGINAGRRACDGDVIIVTNPDVRFGGNAIDALVDADA
                     AVAGPALFWDEGYAWRLPPAELHTARQVASRALASRSRAWAFARDRRRFFARVAFWSL
                     AVPSRVHTLSGAVLAIKAKAFDDAQGFDERFQLYFEENDFLRRVAGDIVYVPAARCHH
                     LYNQSAGESPESAALYAQSESQYLRKWGGEFAKRFERKVPPIAVERLHEHGPRTFDGD
                     VVLEASPLPTFETAAGHFEKGSISDVPDDVWRAYRGNVLYLRAVDRRSGRVLESWAKA
                     RIPA"
     gene            complement(4951..>5281)
                     /locus_tag="VNN25_14090"
     CDS             complement(4951..>5281)
                     /locus_tag="VNN25_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92708.1"
                     /translation="PVHVIVGVHRSADDLQCFAVGTHADGETPPSGRNEYELIAQLRN
                     VPLNRGEYSIIAFAGDEAAITVFDRRDLRRAFSITGERFEVGLISLDHTWSLVPSEME
                     VTAAAGR"
BASE COUNT         1113 a         1665 c         1626 g          877 t
ORIGIN      
        1 tgacgctcgt cggttggagc gtgcgcggat tcgattcctt cggcgacgac gtgcagcgcg
       61 ttgtgaagcg aatcgtgccg cgcgtcactt ccggctcgat cgtggtgatg catcaggggc
      121 gcggcttttc ggtggcgtgc gtgagcagtg tcgtccgcgc gttgcaggcg aagggctacg
      181 cgttcgtcgt tcctctgcct gaccgtttga aaacgaagag gtagttgttg aacggcgtcc
      241 ggccatacaa cggcacgacc tcggcctcga aacccgcaaa tggacgaagg acctcatcgc
      301 gcgtcgcaaa atgcagacgt tccgcgcgaa gccagcgcac caatcgcgag aacatctcct
      361 gcgcgtatgt catccggtag cgccagctcc catcgcgaac ggcgtcgcgg atgatcaccg
      421 tcgtggcatg tgcggcagcg gctgcaagaa ttcgtgactg ctcctccgac gtgaagtaat
      481 gcaacagatc gagcagcaca acggtgccgc cacgatccat cgccacccgc gcatcgccag
      541 cttcaaacgt tgcgtcgggc acgacctgcc gggcaatgcc gatctttcgc tcatcgtgat
      601 cgagaccgca gatgggcgcc gtaaaaccgc gctctcgcag atagctcgga aggatgccga
      661 cgccacagcc gacatcgagc aacggctggc cgctgattcc gagacgatcg aagaccgcgt
      721 cgtaaacggg atccatgcgg atcttcgcgc gaacataaat gcgaagaaag cgcgtcgggt
      781 agcgagcggc cactcgatcc gcaaggccag cgcggctcat acgattcgac gccaggaccg
      841 tcaaggcgaa agatgctcga ttaggtcgat cagctcggga aggttgaacg gcttcggcag
      901 aattgcagaa acgccgagtt gcaagatggc tttcggttcc atgtacaggt gcgcagagag
      961 aacgataatt cgggcggtgc tgaggaagcc cgtctcccgg agccgctgca gaaaatccca
     1021 gccattggtg ccgggcatcg ccaggtcgag gaggatgacg tcgggctggt gaagcgtcag
     1081 agcgcgatat ccctcgtctc cgtcgcaggc aagcaacacg tcaaagcctg cctgccgcaa
     1141 cgcagccgcg agcatgccgc gcaacggcag atcgtcgtca atgacgagca cgcgtttgcg
     1201 gtcgacgtct gcgacgcgct tctcgtcgtc cggatcgtga tgctggactt cactcatgtc
     1261 tcaggcctgc cgtacacatt ctaccgactg ctgatgctgc catgcaggag gctggccact
     1321 gcgggtgtcg ctggtcgggt gtcgggtgtc ggagttcgaa gcagaggctg cccttctacg
     1381 actgactccc gatcagcgac tccctcacga ggattctgct ccgacccccg actcccgaac
     1441 ggcgactccc gcactagaaa agatcgctgt cgccatacgt gtagtacagc cgctcagcaa
     1501 tttgtgcgct tgcctcaggc ttatcaaagg gcacacacat cagagagaga tcctgcgggt
     1561 cggggcggga ctcgaaacca agcccacgca gcaggccatc gaaccatgcc ggccgcgcgg
     1621 gaaaccaacc gtcgatgcga tcgacagggt aggccggcac aacatgacga aggagcactt
     1681 ccacggcgtg gggatagcgc ggatcgaaca acgcgtcacc ccatgccagc ctgcgaccgc
     1741 ggacgccgaa cacgctccag cccaccagcc ggcgccactt ccggatcgca acgacgaagt
     1801 agggcacgtc aggacattcg agataacgcc agcgcagata gcgggaatcg cggcgcacga
     1861 gcaaacgata cgcgggcgca acgcgctcga aaaagtcgtc gtactccgag tcaaaaccgc
     1921 ggaccaggtc gagctgccat ccgcgcggcc agcgctcccg ccgcccaatg cgcggaaatg
     1981 gattcaccgt cagatcgcgc acgcggtacg gcgccggctc cacagcgtcg ctccccagaa
     2041 agcggaccga cagctcgcgg tgtgcggccg tcgtaaagcc atagttgaat gccaccctac
     2101 cgcggcagaa cgtgtcgtaa aaatgactcg ccgttcgggc cagaatgctg gtcggtccgc
     2161 gaccgatgtg gcgcacggag cggtcggtca tgatgtcgcc gatctggttg gcggcgagtg
     2221 cacgtccatc atcgacaagc ggcacgggat agccggcgta ctgcgccacc agccgctggg
     2281 cctcatcgaa cgtcagcgaa atgcgctcgt tgccgaaggg actgcgctgg tactcccact
     2341 gaaaatgttc acgccctcgg gcagcgtgcg gaaaggcccg cgcgaagaga gcgaggatct
     2401 gctcttcatc gccctcgcga aatgcgcgaa tggtgaagcg cgcgtcaacg ggcatgtgtc
     2461 ggctcgacgg atgggatcgc gcggaagttc accggcgatc cgacacgccg tttggccacg
     2521 atgcggatcg atcccgacgg aaccagcagc ggatctggca gaatcctgaa caaggtccgc
     2581 atcacgggca tcagcgcagg ccaccgggtt gccaccgaat gccgaagaag gcgctggtcg
     2641 cggtactcga gctccgcgcc gatgctgaca atctcgaacc cggaatgctc gagcaactcg
     2701 atgagcgcgg agcgcgagta gagaatgaga tgcgtgcgga agttctgcgt catccatcgc
     2761 cgctcggtga tgagatacag caatccctcg gtgtcgaacg tcgagagcag taaaacgccg
     2821 cccggcttca acaggtcctt cattcgcgac aacgcatcgt gcggatcggc catgttctcg
     2881 aagacatgaa aaagcgcgat ggcgtcgaaa gccggtcccg tgttgacgaa gttcgggacg
     2941 gtggagcaat acacctcgag cccgcgggcg cggactgcac gggcaacact cggagcgcgg
     3001 tcgattccga cgtactcaaa ctccgctgcc gccgcggaac cgaagctgcc atcgcgaaca
     3061 ccgacttcga gtatccgagc ccgcgagatg agatgcttgc gcaacgcagc cagacgcctc
     3121 tggctcgtct tctctcgtgc cacgccgcgg tgaagcgtct catcgcccgg atcggctggc
     3181 gctggctggc cacgcagaac gcgccgaacc ggaccgccat cctcgacgac atcgaaatgt
     3241 ccaagatacc ggcacttgca acggctgcac gaccgcacga cgttaccggg gatctgatcg
     3301 atgctccata cttcgttgga gaaacagacc agacagcgcc tcggtgggcg ataaccattt
     3361 cttcgatcgg tcatccggat cgcgacggca gcagcgacgc cggaccagag atcgaacgtt
     3421 gccttcgaac cagacgtcgc gccgtgtatg gtcaggcact ccacgcggaa tccgtactta
     3481 ttcgcgaggt aggccgcttc gagatcgaat cccccttttc gaagcctggt ctcaccaacg
     3541 atcaggcgcg ctgcatcggc ggagaacgcc ttcaggtgta gcgctcgatc gggaagaatt
     3601 ggaacgagaa gcgttcgcag caagagatct cccggtcgcg tctcgcgtcg cccgaaaacc
     3661 acatcggctg ctcctgattg gatcagcgcg acggcatcgc cgatggcact catcggataa
     3721 ggcagatcgt ggtccgcgac cacgatgatg ctgccacctg cctcggccac gccgcgcctc
     3781 aacatggcac cataaccagc accgtcacgg cgatccagga cccgaatgtc gaattcgaaa
     3841 ccggtcgcct gcaggtagcg ctcgatcgcc tcgagcgtcg acggaagctc ggcggacggt
     3901 ccaaccagcg gaacaaggac cgagactctg gtcgagctca tgccggtatt ctagcttttg
     3961 cccacgattc cagcactcgg ccgctgcgtc ggtcgacggc acgcagatag agaacgttgc
     4021 cgcgataggc acgccagacg tcatcaggaa catcggagat cgagcccttc tcgaaatgac
     4081 cggctgcagt ctcgaacgtg ggtagcggcg acgcctcgag caccacatcg ccgtcgaagg
     4141 tacgcgggcc gtgctcgtga agtcgttcca ctgctattgg tggaaccttg cgctcaaacc
     4201 gcttcgcgaa ttcacctccc cacttgcgca gatactggct ttcggactgc gcatacagtg
     4261 cggccgactc cggagactcg cccgcgctct ggttgtaaag atggtgacag cgagcagccg
     4321 gcacataaac gatgtcgccc gcgacgcggc ggaggaagtc attctcttca aagtagagtt
     4381 gaaagcgctc gtcgaatccc tgcgcgtcgt cgaatgcttt tgctttgatc gcaagcaccg
     4441 cgccggacaa ggtgtggacg cgtgatggaa cggcgagcga ccagaaagcc acgcgcgcaa
     4501 aaaagcgccg cctgtctcgt gcaaacgccc acgcgcgcga gcggcttgcg agcgcacggc
     4561 tcgccacctg acgcgcggta tgcaactcgg caggcgggag tcgccatgca tagccttcgt
     4621 cccagaacag cgccggcccg gcgacggctg catcagcgtc aaccagcgcg tcgatcgcat
     4681 tccctccaaa ccggacgtca ggattggtga cgatgatgac gtcaccgtca caagcgcggc
     4741 gtccggcgtt gatgccaccg gcataaccga gatttcgagg cgcggtaatc accacgtccg
     4801 caacgggacc aagcgcacgc gcttcggacg cgtcggtgct gttatccacc gcgaccacct
     4861 gaagcggctg cgatgacgaa gcgcgcgcgg ttctgatggc atcgattgcg agcgatgccg
     4921 accgatagtt gatgagcagg agtgaacgct tcatctgccg gcagcagcgg tgacttccat
     4981 ttccgacggc acaagcgacc atgtgtgatc gagcgagatc agtccgacct cgaaacgctc
     5041 gccggtgatc gagaacgcgc ggcgcaaatc acggcggtcg aataccgtga ttgccgcctc
     5101 gtcccccgca aaagcgatga tcgagtattc gccgcggttc agcggaacgt tcctgagctg
     5161 cgcaatcagc tcgtactcat tacggcccga tggcggcgtc tctccgtccg catgtgtgcc
     5221 gacagcgaag cattgaaggt catcggcgga gcgatggacg cccacgatca catgtacggg
     5281 c
//
LOCUS       DATLVW010000466         4054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_555906, whole genome shotgun sequence.
ACCESSION   DATLVW010000466 DATLVW010000000
VERSION     DATLVW010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555906"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..727)
                     /gene="phoU"
                     /locus_tag="VNN25_14095"
     CDS             complement(50..727)
                     /gene="phoU"
                     /locus_tag="VNN25_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXH92709.1"
                     /translation="MIVPRHFDKDIDELKHLLLRMGAMVEDAMARSIKALLERDSALA
                     EEVIANDDEIDRMEVLIDQKTIELIAKMQPAASDLRFVATIMKITPELERIADLAQDV
                     CERAIELNREPLLRPLVSLPQLAYDAQAMVREALNAFVRGDADLARRVITQDDNVDAL
                     TESSFRSLLTYMLEDARNISSAIRLTFIGKYFERMADGATNICEMVVYLVEGKMIKHT
                     STIAAAK"
     gene            complement(724..1485)
                     /gene="pstB"
                     /locus_tag="VNN25_14100"
     CDS             complement(724..1485)
                     /gene="pstB"
                     /locus_tag="VNN25_14100"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXH92710.1"
                     /translation="MSQDAKISVSNFDFYYGTAKVLHGINLDIPDRKVTALIGPSGCG
                     KSTFIRSINRMNDIIPDAHASGTITIDGQDVYSGSADVVELRRRVGMVFQKSNPFPKS
                     IYDNVAYGLKIGGMRNKRQLDEIIERSLTRAALWHEVKDRLHASALALSGGQQQRLCI
                     ARALAVDPEILLMDEPASALDPIATSKIEELIYELKDNYTIVIVTHNMQQAARVSDRT
                     AFFMLGRLIEYAETNEIFMNPKEKMTEDYVTGRFG"
     gene            complement(1764..2615)
                     /gene="pstA"
                     /locus_tag="VNN25_14105"
     CDS             complement(1764..2615)
                     /gene="pstA"
                     /locus_tag="VNN25_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXH92711.1"
                     /translation="MTANLRRRKLFSRFFEILCGLAVVIALIPLAMILFYVIKEGAGA
                     MNFDFFTKMPKPVGESGGGMANAIVGTLILIGIAALFAVPVGGICGIHLAEYPGTKFS
                     SVVRFAADVLNGVPSIVVGIFAYGILVLPVKRFSAIAGGVALGLLMVPIVVRTTEELI
                     RLVPGGLREGALALGATRSRAVFGVILPAALPGILTGILVALARVAGETAPLLFTAFN
                     NRFWSTSLTQPIASLTVQVFTYAISPYDDWHRQAWAGAFLLVMIILTLSIAARLAVRR
                     LERMNRS"
     gene            complement(2612..3568)
                     /gene="pstC"
                     /locus_tag="VNN25_14110"
     CDS             complement(2612..3568)
                     /gene="pstC"
                     /locus_tag="VNN25_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXH92712.1"
                     /translation="MKRSSDDRSFRIGTGAFAALVLLIVLGIAFELTRQSALSITKFG
                     LKFWTTSTWDPVSGDFGALPFIWGTLYSSILALLLATPIALGIAIFISELSPRVLRQP
                     LAYLTELLAAIPSIVYGLWGVFVLVPLVRKLELATPAAFKKIPLFSGPPLGVGMLAAA
                     LILAVMVIPFTSSVAREILKNVPSTQREAAYALGATRWEAIKVAIGFGKSGIIGAVML
                     GFGRALGETMAVTMVIGNSPQVSASLFKPQYTMAAVIANEFTEAADELYLHALIEIGL
                     LLFIITVGVNLLSRILIWSTKRRAIAPPVVTPTPIVAEEVAA"
     gene            complement(3565..>4054)
                     /locus_tag="VNN25_14115"
     CDS             complement(3565..>4054)
                     /locus_tag="VNN25_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXH92713.1"
                     /translation="KVSPDFKEKVGVATSVNWPAGVGAKGNEGVSGLVQQTPGAIGYV
                     ELIYALQNKIPYGPVQNKSGQYVTTSLESVTAAAAGVTMPDDFRVSITDPAAADAYPI
                     ASFTWMLLYQHPADKARSKAMVDFLKWALADGQKFAPGLGYAPLPQPVIAKELQALAT
                     VQ"
BASE COUNT          753 a         1201 c         1372 g          728 t
ORIGIN      
        1 cggatcgtga aggcaatgtc ggcgtcgtct tcgatcagcg cgatcttcgt catttcgccg
       61 ccgcaatggt cgacgtgtgc ttgatcattt ttccttcgac gagatacacc accatctcgc
      121 agatgttcgt ggcgccatcg gccatgcgct cgaagtattt gccgatgaac gtgagccgga
      181 tggccgagga gatgttgcgg gcatcttcga gcatgtaggt gaggagactg cggaacgagc
      241 tctccgtgag cgcgtcgacg ttgtcgtcct gcgtgatgac gcggcgggcc agatcggcgt
      301 cgccgcgcac gaaggcgttg agagcttcgc gcaccatggc ctgagcatcg taggccagtt
      361 gcggcaggga gacgagcgga cggagcagcg gctcgcgatt gagctcgatg gcccgctcgc
      421 aaacatcctg ggcaagatcg gcgatgcgct cgagctcggg cgtgatcttc atgatcgtgg
      481 ccacgaagcg aaggtcgctg gcggccggct gcatcttggc gatcagctcg atggtcttct
      541 gatcgatgag cacctccatg cggtcgatct catcatcatt ggcgatgacc tcctcggcca
      601 gcgcagagtc gcgctcgagc agcgccttga tcgagcgcgc catggcatcc tcgaccatcg
      661 ctcccatgcg aaggagaaga tgcttcagtt cgtcgatatc tttgtcgaag tgacgcggta
      721 cgatcatcca aaccttcctg ttacgtaatc ttcggtcatc ttttctttcg ggttcatgaa
      781 gatctcgttc gtctcggcgt attcgatcag gcggccgagc atgaagaacg cggtgcggtc
      841 gctgacgcgc gcggcctgct gcatgttgtg cgtgacgatc acgatggtgt agttgtcctt
      901 cagctcgtag atcagctcct cgatcttcga cgtagcgatt ggatcgagtg ccgaggccgg
      961 ctcgtccatc aggaggatct cgggatcgac ggcgagggcg cgggcgatgc agagccgctg
     1021 ctgctggccg cccgacagtg ccagcgcact ggcgtgcagc cggtctttca cctcgtgcca
     1081 gagtgcggcg cgcgtcaggg agcgctcaat gatctcgtcg agttgccgtt tgttgcgcat
     1141 gccgccgatc ttcaggccgt acgccacatt gtcgtaaatc gacttcggga acggattcga
     1201 tttctggaag accatgccga cgcggcggcg tagctcgacg acgtcggcgc taccgctgta
     1261 cacatcctgg ccgtcgatcg tgatcgtccc gctcgcgtgc gcgtccggga tgatgtcgtt
     1321 catccggttg atggagcgga tgaaggtcga cttgccacag cctgatggtc cgattagcgc
     1381 cgtgactttg cggtccggaa tgtcgaggtt gatgccgtgc aggaccttgg cagtgccgta
     1441 gtagaagtcg aagttgctga cggagatttt ggcgtcttgg ctcatcggcc cttcttgtgc
     1501 tgacggttgg gttcgcgtca ggagctgcgc gtgattggcg tttggcggcg gcgaggacgc
     1561 cgccgggcca gccgccggga cggcagcgtt ccactcgtta gcgtcacgtt cctggcgtat
     1621 ggcgtcagga agcggatagt caccaacagt gcgaggcaat gcatctttgg ccgcacgaaa
     1681 cgaagtgtgg ccagtgcgcg gcggaacgcc ggcgtcctcg ccggctggct cggcggcgtc
     1741 ccgccgccgc agttcggtcg tcatcatgat cggttcatcc tttcgaggcg gcggacagcg
     1801 agacgtgcgg cgatggacag cgtcagaatg atcatcacga gcagaaacgc gccggcccac
     1861 gcctggcggt gccagtcgtc atacggcgag atcgcgtagg tgaagacctg aacggtgagc
     1921 gacgcgatcg gctgcgtgag gctcgtagac caaaaccggt tgttgaatgc ggtgaagagc
     1981 agcggcgcgg tctcgccggc cacgcgcgcg agcgcgacga ggattccggt gaggatgccc
     2041 gggagggcgg cggggaggat gacgccgaag actgcgcggc tgcgcgtggc gccgagggcg
     2101 agagcgcctt cgcgcagacc gccgggaacg agccggatca actcttctgt cgtgcggacg
     2161 acgatcggaa ccatcaacag gccgagcgcc acaccgccgg cgatcgcgct gaatcgtttg
     2221 accggcagga ccaggattcc gtacgcgaag atgccgacga cgatcgaggg aacaccgttg
     2281 agaacatccg cggcgaagcg cacgaccgat gagaacttcg ttcccggata ttcggcgagg
     2341 tggattccac agatgccacc cacaggcacg gcgaagaggg ccgcgatccc gatgaggatc
     2401 agcgtcccga cgatggcgtt ggccattccg ccgccgctct cgccgaccgg cttcggcatc
     2461 ttcgtgaaaa agtcgaagtt catcgcgccg gcgccttcct tgatcacgta gaagaggatc
     2521 atcgccaacg ggatgagggc gatgacgacg gccaggccgc agaggatctc gaagaatcgc
     2581 gagaacagtt tccgccgccg caggttcgcc gtcatgccgc cacctcctcg gcaacgatcg
     2641 gagtcggagt cacgaccggt ggtgcgatag cacgccgttt cgtcgaccag atcaaaatcc
     2701 gcgacaggag gttgacgccg acggtgatga tgaagaggag caggccgatc tcgatgagcg
     2761 cgtgcaggta gagctcatcg gcggcctccg tgaattcatt cgcgatcacc gcggccatcg
     2821 tgtactgcgg tttgaacaac gatgccgaga cctgcggcga attgccgatg accatcgtga
     2881 ccgccatcgt ctcgccgagc gcgcggccga aaccgagcat gacggcgccg atgatgccgc
     2941 tcttgccgaa gccgatggcg actttgatcg cctcccagcg cgtggcgccg agggcgtagg
     3001 cggcctcgcg ctgcgtggaa ggaacgtttt tcaggatctc gcgggccacc gaggaggtga
     3061 aggggatcac catcacggcg agaatcagcg cggccgcgag catgccgacg ccgagcggcg
     3121 gaccgctgaa cagcgggatc ttcttgaacg cggcgggcgt cgccaactcg agctttcgca
     3181 cgagcggaac gaggacgaag acgccccaca acccgtacac gatcgaggga atcgcggcca
     3241 acagttcggt gagatacgcg agcggctggc gcagaacgcg cggcgagagc tcggagatga
     3301 agatggcgat gccgagggcg atgggcgtgg cgagcaggag cgcgagaatc gacgaataga
     3361 gcgtccccca gatgaaggga agcgctccga agtcgccgga gacgggatcc cacgtcgacg
     3421 tcgtccagaa cttcagcccg aacttcgtga tcgacagcgc cgactgccgc gtcagctcga
     3481 aggcgatgcc gagaacgatc agcaacacga gcgcagcaaa cgcgcccgtg ccgatgcgga
     3541 agctgcggtc gtcggagctc cgcttcattg cacagtcgca agcgcctgca attctttcgc
     3601 gatgaccggc tgcggcagcg gcgcgtagcc gagtccgggt gcgaacttct ggccgtcggc
     3661 gagagcccac ttcaggaagt cgaccatcgc cttgctgcgg gctttgtccg ccggatgctg
     3721 atagagaagc atccaggtga atgaggcgat cgggtaagcg tcagcggcgg cgggatcggt
     3781 gatcgagacg cggaagtcgt cgggcatcgt cacgccggcc gccgccgcgg ttaccgactc
     3841 gagcgacgtg gtgacgtact ggccggactt gttctgcacc gggccatacg gaatcttgtt
     3901 ctgcagcgcg tagatgagct cgacgtagcc gatggcaccg ggagtctgct gcacgaggcc
     3961 ggagacccct tcgttcccct tcgcgccgac gccggccggc cagttgaccg aggtagccac
     4021 gccgaccttc tccttgaaat cgggcgacac cttc
//
LOCUS       DATLVW010000467         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_102805, whole genome shotgun sequence.
ACCESSION   DATLVW010000467 DATLVW010000000
VERSION     DATLVW010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102805"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VNN25_14120"
     CDS             complement(<1..342)
                     /locus_tag="VNN25_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HXH92714.1"
                     /translation="MFDNLQDKMDRVFKTLRGEAALNEWHIDNALKEIRVALLEADVH
                     FKVVKEFTNRVREKAVGQDVLTAFSPAQQVTKVVRDELQELLGGNEVGLALVGSPAVV
                     MMVGLQGSGKTT"
     gene            complement(382..1278)
                     /locus_tag="VNN25_14125"
     CDS             complement(382..1278)
                     /locus_tag="VNN25_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92715.1"
                     /translation="MADRRRALIWFAALLALVFAGDRVLAWALGRVMVRSQFRFSRLY
                     RGGNDADIVILGDSRGVHSFYQPEIEKLTGLRTFNLSYNSMSTRIAEAVLRDYLDRNR
                     APRLVIIEATCTIPEGALAGQLGTYASFSPRLAALYAEGHPDGALTGRLFHLLRLNSE
                     FYFEALHYQTRSDQDWINRSTIPEALRIRQPGLWSPPRAENLEALNRIVTLLKQRGIE
                     VRLVIAPYWPVPVNIPEIANAIDVSIRPADPQLHVLNYAGAVTDPEEFADGVHLNERG
                     SVALMEILQRDGVFAIHSAAPR"
     gene            complement(1280..2755)
                     /locus_tag="VNN25_14130"
     CDS             complement(1280..2755)
                     /locus_tag="VNN25_14130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXH92716.1"
                     /translation="MRFNSLAFLAFLPLFAAGYWLTSGRVRLWFMLIASLFFYAWWDW
                     RFVFLLLFSSVVDFSLGIFLENEQDDRRRRRLISLSIAVNLGLLGFFKYFNFFVVSAV
                     DLCRSLGLPASEKTLHIILPVGISFYVFKTMSYTIDVYRRTEKAERDLLRFTTFVVFF
                     PELVAGPIVRASRMLPQLQRDHRFRFEQMVSGLTLVASGYVRKVAIADSIAPFVDICF
                     AHPEAHNALSLLIGVYFYAFQIYCDFSGYSSIAIGLARIFGFDFGINFDRPYFSRSFS
                     EFWTRWHISLSSWLRDYLYIPLGGNRGGRWATLRNLFITMLLGGLWHGASWTFVAWGA
                     LHGIYLVVQRLLAPAWNRVTRALRIPALASNAFLVFLIFHLTCFAWIFFRASSFANAW
                     QVLSGIVSVHGPQLAGLENRGRIAKCALMVAILLVFEAMAFLPKQRKAVLATTEGEKV
                     TVSAHPLEASPALRLVFIAACIWVVLIFGSFNGNNFIYFQF"
     gene            2822..3697
                     /locus_tag="VNN25_14135"
     CDS             2822..3697
                     /locus_tag="VNN25_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH92717.1"
                     /translation="MAQLAQRLPENVPGDFFVDSTCIDCDACRIFAPKTFSDAGDQSS
                     VYHQPENEEELLAAQKALISCPTASIGSVAKLDMRQALDDLPELVDDDVFRCGYASES
                     SFGAISYLIQRTDGNVLIDSPRFSGPLVKNIEAMGGVSKLLLTHQDDVADHAKFHDRF
                     RCERVLHRDDVHSRTASIELQPAGIDPIILGNDIVMIPTPGHTKGHAVFLYRDRYLFT
                     GDHLAWSERRGHLYAFRSACWYSWSEQIRSMERLLDYRFEWVLPGHGRPVHLDAAAMR
                     ASLERCISWMRGIDN"
BASE COUNT          826 a         1139 c         1180 g          627 t
ORIGIN      
        1 cgtggtttta ccggagccct gaaggccgac catcatcaca accgcgggcg agccgaccag
       61 cgccaggccg acttcgttgc cgccgaggag ctcctgcagc tcatcgcgga cgactttggt
      121 gacctgttgg gcgggcgaga aggcggtgag cacgtcctgg ccgacggcct tttcacggac
      181 gcggttggtg aactctttga cgactttgaa gtgaacgtcc gcttcgagca gcgctacgcg
      241 gatttccttg agcgcgttgt cgatgtgcca ctcgttgaga gcagcctcgc cgcgcagggt
      301 cttgaagacc cggtccatct tgtcctgcag gttgtcgaac atgcggaggg ttaaacgccc
      361 tcaccgtcga aaaaatccgg ctcaccgcgg cgccgcggag tgaatcgcaa agaccccgtc
      421 ccgctgcagg atctccatca gggcaacgct cccgcgttcg ttgaggtgaa cgccgtcggc
      481 gaactcctcg gggtcggtta cagcgcccgc gtaattcagg acgtgcaact gcgggtcggc
      541 ggggcggata gaaacatcga tcgcattcgc aatctccgga atgttgaccg gcaccggcca
      601 gtagggagcg atgaccaatc ggacctcgat cccacgctgc ttcaggagcg tcacgatgcg
      661 gttcaacgcc tcgaggttct ccgccctcgg cggcgaccaa agccccggct gtctgatccg
      721 aagcgcttcc ggaatcgtcg atcggttgat ccaatcctga tcggaccgag tctggtagtg
      781 cagcgcctca aaatagaact cgctgttgag acgaagaaga tggaagagac gcccggtgag
      841 cgcgccgtcc ggatgcccct cggcatacag agcagcaagc cggggcgaaa aactcgcgta
      901 cgttcccaac tggcccgcga gcgccccctc gggaatcgta cacgttgcct cgatgatgac
      961 caggcgcggc gcacgattgc ggtcgaggta gtcgcgcaga accgcctcgg caatccgcgt
     1021 cgacatgctg ttgtaggaga gattgaacgt tcgcagcccg gtgagtttct cgatctccgg
     1081 ctggtaaaac gaatggacgc cgcgcgagtc gccgaggatg acgatgtcgg cgtcgttgcc
     1141 gccgcgatag agcctcgaaa agcggaactg gcttcgcacc atcacccgac cgagcgccca
     1201 ggccaggaca cgatcgccgg cgaacaccaa cgccaggagt gcggcgaacc agatcagtgc
     1261 gcgtcgtcgg tcggccatgt cagaactgaa agtagatgaa attgttgccg ttgaacgagc
     1321 cgaagatcag cacgacccag atgcacgcgg cgatgaacac cagccgcagc gcgggcgacg
     1381 cctccaacgg atgcgccgaa accgtgacct tctctccctc ggtcgtcgca agcaccgcct
     1441 tgcgctgctt cggcagaaat gccatcgctt cgaacacgag aaggatcgcc accatcagcg
     1501 cgcatttggc gatgcgtcct cggttctcga ggccggccaa ctgcgggccg tgaacggaaa
     1561 cgattccgga caggacctgc catgcattcg cgaacgaaga cgcgcgaaag aagatccacg
     1621 cgaagcacgt caggtgaaag atcagaaaga cgaggaaagc attcgatgcg agcgcgggga
     1681 tgcgcaacgc gcgggtcacg cgattccagg caggtgcaag caggcgttgc acgacgaggt
     1741 agatgccgtg cagcgcgccc catgcgacaa acgtccaact cgcgccgtgc cacagcccgc
     1801 cgaggagcat ggtgatgaag agattgcgga gggtcgccca gcggccgccg cgattcccac
     1861 ccaagggaat gtagagatag tcgcgaagcc agctcgaaag ggagatgtgc cagcgcgtcc
     1921 agaactcgct gaagctgcgc gagaaatagg ggcggtcgaa gttgatgccg aagtcgaatc
     1981 cgaagatacg cgccagtccg atggcgatgc tcgaataacc ggaaaaatcg cagtagatct
     2041 gaaacgcgta gaagtagacg ccgatgagca gcgacaacgc gttgtgcgcc tcgggatgcg
     2101 cgaagcaaat gtcgacgaac ggcgcgatcg agtccgcgat cgccaccttc cgcacgtagc
     2161 ccgaagcgac cagcgtgagg ccggacacca tctgctcgaa gcggaaccgg tgatcgcgct
     2221 gcaactgcgg gagcatgcgt gacgcacgaa cgatcggacc ggccacgagc tccggaaaga
     2281 agacgacgaa ggtcgtgaat cggagcagat cgcgctcggc cttctccgtc cgccggtaga
     2341 catcgatcgt gtagctcatc gtcttgaaaa cgtagaacga gatgccgacg ggcagaatga
     2401 tgtgcagcgt cttctcgctc gccggcagcc cgagcgagcg gcaaagatcg accgccgaca
     2461 caacgaagaa gttgaagtat ttgaagaatc cgaggagtcc gaggttgacg gcgatgctga
     2521 gggagatcag gcggcggcga cgtctgtcgt cctgttcgtt ctcgagaaag atccccagcg
     2581 agaagtcgac cacgctggaa aacaggagca gaaaaacgaa gcgccagtcc caccaggcat
     2641 agaagaagag gctggcaatc agcatgaacc aaaggcggac ccggccggac gtcagccaat
     2701 agcccgcggc gaagagcggc aggaacgcga ggaaagccag gctgttgaaa cgcatcggtc
     2761 tgttggagga gcggcattct aacggcgaaa ttgcgatcgc tgcgccgtcg ttttgtctcc
     2821 cgtggctcag cttgcgcaac ggcttccgga aaatgtgccg ggcgatttct ttgtcgacag
     2881 cacgtgcatc gactgcgacg cctgccgcat cttcgcgccg aagacgttca gcgatgccgg
     2941 cgaccagtca tcggtctacc accagcccga gaacgaagag gaactgctcg ccgcgcagaa
     3001 ggcactcatc tcctgtccga ccgcatcgat cggatcggtc gccaagctcg acatgcgtca
     3061 ggcgctcgac gatcttccgg agctggtcga tgacgacgtc ttccgctgcg gctacgcctc
     3121 ggagtcgtcc ttcggcgcga tcagctatct cattcaacga acggacggca acgtcctgat
     3181 cgactcacca cgcttctcgg ggccgctcgt gaagaacatc gaggcgatgg gcggcgtgtc
     3241 gaaactgcta ctcacgcatc aggacgatgt cgccgaccac gcgaagtttc acgatcgctt
     3301 ccgctgcgag cgcgttctgc atcgcgacga cgtgcattcg cggacggcat cgatcgagct
     3361 tcagccggcg ggcatcgatc cgatcatcct cggcaacgac atcgtgatga tcccgactcc
     3421 cggccacacg aaaggccacg cggtgtttct ctatcgcgac cgctatctat tcaccggcga
     3481 tcatcttgcc tggagcgagc gccgcggcca cctctacgca ttccgcagcg cctgctggta
     3541 ctcgtggagc gagcagatcc gctcgatgga gcgattgctc gactatcgct tcgaatgggt
     3601 cctaccgggt cacggccgcc cagtccatct cgatgccgct gcgatgcgcg cgagtctgga
     3661 gcgatgcatc agctggatgc gcggaattga taattgacaa tggacaattg acaactgaaa
     3721 gcggagacgg caagaggcgg caagcttctt gataatgccg gtctgagtgt cg
//
LOCUS       DATLVW010000468         7795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_229746, whole genome shotgun sequence.
ACCESSION   DATLVW010000468 DATLVW010000000
VERSION     DATLVW010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229746"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..7413)
                     /locus_tag="VNN25_14140"
     CDS             complement(<1..7413)
                     /locus_tag="VNN25_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HXH92718.1"
                     /translation="MNANRNVAARLLAFGLTRVPHSYGEGIVRRTALVLAAVFCTFMM
                     SRAAQAQVPILYYDFENNTTRTVFENLVEQSVNTGSGALTKVGGTVTISGVGGAGTFN
                     GGAAAGEALTAAGWDSSTTDPGAAAGFYYQFVVNTTGFSGLTVSFDCQASSTGPGRVG
                     VLFSTDGTTFTTTSTGLVTSATFTGNSQTFTLGAGANNQPSVTIRIYPFAGSAADRAT
                     VPAHAAFSAAGTFRIDNLTVSSNTVTATKTLLDYAAVGLSVKSGTAFLPTYANLAVNG
                     SGINVTLASNLTLSGTLALNAGNIVTAANTLALTGTASTTSGSSSSYVIGNLKRTFSA
                     ASSKIFDAGTANGYSPVTANVTAGTFPLDLTVVAVQAPQPNVSAGNSIQRYWTLTATN
                     GPTGDLTFQYLAGDVQGNEANYQLARVSGGVPTFFPGTVTPATHTAALAGVSSFSDWT
                     VGEPVDTTPPAVTSVSVPANGTYVAGQNLDFTVNFSETVNVTTGGGTPRIPITLNTGG
                     TVYASYVSGGGSSALFRYTVVTGNEDPDGIVVGAAFEANGGTIKDAAGNDALTTLNGV
                     GSTTGVKVDAIAPTVSSSTVPADGTYVAGQNLDFTVTYSEPVIKTGSPFVGVTLNTGG
                     AVQAAYVSGTGTATLTFRYVVASGNLDADGISVDPSITLNGGTIKDAAGNNAATSVSF
                     ASTSAVFVDAVAPTVSSSTVPANGTHIIGQTLDYSVTFSEPVFVAGGTPSITVNLDGG
                     PVLATYLTGSGSATLQFRYTVVSGDTAPGGVSTASFIALNGSTIKDFAGNNANTAVTF
                     ASTAGVLVDGIAPIVLTSVRADADPTNSPTLHYTVTFSEDVSGVTTGAFALTAGGGAS
                     GSITGVVPVNAHTYTVTVGSAAGEGTLRLDVNASGTGITDLPGNPLNGGFTTGQSYTL
                     DHVGPNVTSVAVPAAITYTTGQNLDFTVNFNENATVASGTPRIALTLNSGTVYANYVS
                     GSPGTALLFRYTVVSGDSDPDGIVVAGSIDLNGATIRDALGNDGNTTLNSVGSTTGVL
                     VDANAPAVLSVTRASSNPTNAASVDFTVTFSQGVTGVDAGDFTLTTTGGITPGGISVS
                     PVNSSVYTVSVGTGTGDGTIRLDVANDGSIHSESNNAPLGAAFTTGEVYTIDKTAPAV
                     QSIVRANSSPTHASSVDFTVTFSESVTGVASNDFALLMSGVSGATIANVAGSGSTYTV
                     TVNTGSGDGTLQLDFLGSAADSAGNATSGAFTSGQSYTIDKTPPTVQSITRVGAATTN
                     AASVDFTVTYSEPVTGVSAQDWTLTTTGVTGASVTATNGSGTTYTVTVNTGSGDGTIR
                     LNVVTGGTVIDLAGNLLQAGFTSGDVDTIDKTAPAVQSIVKASTDPTTATSVDFTVTF
                     TESVTGVDATDFAITAPALTGTSVSNVTGSGTTYTVTVATGNGSGSLRLDLTGNGTVV
                     DTAGNSAASYTGGPSYTIQGLPAAPTTLVASGGDSHILLTWNPPSGADTYTVKRSLTS
                     GTGYSNLATGIAVTNYDDTSTANGTPYFYKVSATNGRGEGPDSSEATATPAPAGPPPG
                     PVSIAVGDAKVILSWAPVGGATSYNVKRGTTSGTYTVTTNTPVPTFTDPTVVNGTTYY
                     YVVTALSPSESAPSAEVSGTPNTPSVLGVVISTVYGGGGNSGSTLKNDYVELFNRGTQ
                     TVALSGWFVHYSSATATTWNSPPSAGTTPTALSGTIAPGHYYLVQEAAGAAGTVNLPT
                     PDATGTIALSATAAKIALADTATIGVQCPTGGSIADFVGYGPTANCSETSPTAVLANT
                     TAALRKNNGCTDTNNNNSDFTVGAPNPRNSSSPVNVCGAVNNPPAITPPANPIASVAQ
                     DAAPFTVNLSGTDDGGVYQWTATPGTGLQSVTVSGGQGTTTVTYTVTLTAGFSGTATF
                     TASLSDGINAAVTQLVNIQVTGTGGNNPPLITPPANPITTVAQDPAPFTVNVSGTDDG
                     GIFNWTATPGTGVASVNVTGGQGTATVTYTVALNAGFNGTATFTASLSDNVNPTANQA
                     VNINVLPAGATPNHVVISQLYGGGGNSGATYTNDFIELFNPTASPVSLAGWSVQYSSA
                     TNTGAFSGLQPIGGTIGPGEYYLISLASGGAIGSPLPAANVVNNSGINMSGTTGKVAL
                     VNTGNPVPGPCSATAADTEIVDLVGYGTANCNEGGTNAPAPSNTTSDFRKNGGNTDTN
                     VNGNDFLTGTPNPRRTTPIQEFGPTVVNTDPVSNASAAPRDASMIITFSELVDVDPGW
                     YNINCTSTGNHNDATVVEGAPNTWVITPNANFVAAEQCTVTILQNSVHDRDTDDSAPG
                     TDTLPADYSWTFTVSSGTLPPYPPSVHLTMGQPSVCTTSLSSPNAYLMLKPENSICYN
                     RDLGRPNWVSWHLSDEWVGSLTRVDTFRPDPEVPPTWYRVLASDFFASGFDRGHMTPN
                     ADRDKETSIPINQATFLMSNMVAQAPDNNQGPWANLENYLRSLLSVDEL"
BASE COUNT         1280 a         2200 c         2776 g         1539 t
ORIGIN      
        1 cagctcatcg acagaaagga gagaacgcag atagttctcg aggttcgccc acggtccctg
       61 gttgttgtca ggcgcctgcg cgaccatgtt cgacatcagg aacgttgcct ggttgatcgg
      121 gatcgatgtc tccttgtcgc ggtcggcatt cggcgtcatg tgcccgcggt caaagcctga
      181 cgcgaagaag tcgctggcca gtacgcgata ccaggtgggc ggaacttccg gatcgggccg
      241 gaacgtgtcg acacgcgtca gcgagccaac ccactcatcg ctgagatgcc agctcaccca
      301 gttcggacgg ccgaggtcac ggttgtagca gatcgaattc tcgggcttca gcatcaggta
      361 ggcgttcgga gatgacaggc tggtcgtaca aaccgatggc tggcccatcg tcagatgaac
      421 ggatggcgga tagggaggca gagttcctga ggagacggtg aacgtccacg agtagtcggc
      481 gggcagcgtg tcggtgcccg gcgcgctgtc gtccgtgtcg cggtcgtgca cggagttctg
      541 gaggatcgtc accgtgcact gctccgcggc gacgaagttg gcgttgggcg tgatcaccca
      601 tgtgttcgga gcgccttcaa cgaccgtggc gtcgttgtgg ttgccggtcg acgtgcagtt
      661 gatgttgtac cagcccggat cgacgtccac cagctcggag aacgtgatga tcatgctggc
      721 gtcgcgaggc gcggcggagg cgttgcttac gggatcggta ttcacgacgg tcggtccgaa
      781 ctcctggatc ggcgtcgtgc gccgcggatt cggcgttccg gtgaggaagt cgttgccgtt
      841 gacgttggtg tcggtgttgc cgccgttctt gcggaagtcg gacgtcgtgt tgcttggtgc
      901 cggcgcgttc gtgccgcctt cgttgcagtt cgcggtgccg tagccgacca gatcgacgat
      961 ctcggtgtcg gcagccgttg cggagcaggg accgggcacc ggattgccgg tgttgacgag
     1021 agcaaccttg ccggtcgttc cgctcatgtt gataccgctg ttattgacga cgttcgcggc
     1081 ggggagcggg ctgccgattg cgccgcccga ggcgagcgaa atcaggtagt actcgcccgg
     1141 tccgatcgtg ccgccgatcg gctgcagacc ggagaacgcg ccggtgttgg ttgccgatga
     1201 atactgcacg ctccatcccg ccaggctgac cggagaagcg gtggggttga agagctcgat
     1261 gaagtcattg gtatacgtcg cgccggagtt gccgccaccg ccgtagagct ggctgatgac
     1321 gacgtgattc ggcgtcgcgc cggcgggaag cacgttgatg ttcacggcct ggttggcagt
     1381 cgggttgacg ttatcggaga gcgacgcggt gaacgtcgcc gttccgttga atcctgcgtt
     1441 caacgcgacc gtgtacgtga ccgtggccgt gccctggccg ccggtgacat tcacgctggc
     1501 cacgccggtt cccggagtgg ccgtccagtt gaagatgcca ccgtcatcgg ttcccgacac
     1561 gttcaccgtg aagggagcag gatcctgcgc cacggtggtg atcggattcg caggcggtgt
     1621 aatcagcggc ggattgttgc caccggtccc tgtgacctga atgttgacga gctgcgtgac
     1681 cgctgcgttg attccgtcgc tcagcgaggc ggtgaacgtc gccgttccgc tgaacccggc
     1741 cgtcagagtg acggtgtacg tcacagttgt agttccctgg ccgccgctga cggtcacact
     1801 ctgcaggccg gtgcccggag tggccgtcca ctgatagacg ccgccgtcgt cggtaccgct
     1861 gagattgacg gtgaacggcg cggcatcctg agccaccgat gcgatcggat tcgccggcgg
     1921 cgtgatcgcc ggcgggttgt tgaccgcgcc gcagacgttg accggtgatg agctgttgcg
     1981 cgggttcggc gcgccgaccg tgaagtcgga gttgttgttg ttcgtatcgg tgcagccgtt
     2041 gttcttgcga agtgcggccg tcgtgttggc aagcaccgcg gtcgggcttg tctcggagca
     2101 attggcggtc ggaccatagc cgacgaagtc tgcgatcgag ccgccggtcg ggcactggac
     2161 gccgatcgtc gccgtatcgg caagggcgat cttggcggcc gtcgcgctca acgcgatcgt
     2221 tcccgtggcg tcgggagtcg gaagattgac cgtgcctgct gctccggcgg cttcctgcac
     2281 gaggtagtag tggcccggtg cgatcgttcc ggaaagtgcc gtcggggtcg tgccggctga
     2341 cggaggtgag ttccatgtgg tcgccgtggc cgacgagtaa tgaacgaacc agccgctcag
     2401 tgcgacggtc tgcgtgccgc ggttgaagag ctcgacatag tcgttcttca gcgtggagcc
     2461 ggagtttccg ccaccgccgt agacggtgct gatgacgacg ccgagcaccg agggcgtatt
     2521 cggcgtcccg ctcacctcgg ctgacggtgc gctttcgctc ggcgagagcg ccgtgacgac
     2581 gtagtagtac gtggtgccgt tgacgacggt cgggtcggta aacgtcggca ccggcgtgtt
     2641 tgtggtgacg gtatacgttc cgctcgtcgt gccgcgcttg acattgtacg aagtcgctcc
     2701 gcccacgggg gcccaggaga ggatgacctt cgcatcgccg acggcgatgc tgacgggtcc
     2761 gggcggggga ccggcggggg cgggcgtcgc ggtggcctca ctcgagtcgg gaccttcgcc
     2821 gcggccgttt gtagcgctga ccttgtagaa gtacggcgtg ccgttggccg ttgacgtgtc
     2881 gtcgtagttg gtgacggcga tgccggtggc caggttcgaa tacccggttc cgctcgtgag
     2941 cgagcgcttc acggtgtagg tatcggcacc gctgggtggg ttccatgtca gcagaatgtg
     3001 cgagtcgccg cccgatgcca cgagggtcgt cggcgccgcg ggcaggcctt gaatggtgta
     3061 gctcgggccg ccggtgtagc tggcggcgct gttgcctgcg gtgtcgacaa cggtgccgtt
     3121 gcccgtgagg tcgagacgca gcgaaccgct gccgttgcct gtggccacgg tgactgtgta
     3181 cgtcgtgccg ctgccggtga cgttcgaaac cgaggtcccg gtaagggccg gtgcggtgat
     3241 ggcgaagtcc gttgcgtcga ctccggtaac gctctcggtg aacgtaacgg tgaagtcgac
     3301 cgaggtcgcg gttgtcggat cggtgctggc tttgacgatc gattgcaccg cgggagccgt
     3361 cttgtcgatc gtgtcgacat cgccgctggt gaagcctgcc tggagcagat tgcccgcgag
     3421 gtcgatgacg gtaccgccgg ttacgacgtt gaggcggatc gttccgtcgc cgctgccggt
     3481 attgacggtg acggtgtagg tcgtgccgct gccgttggtt gcggtgaccg atgcgccagt
     3541 cacgccggtc gtcgtgagtg tccagtcctg ggcggagacg ccagtgacgg gctcggagta
     3601 cgtgacggtg aagtcgacgc tcgctgcgtt cgtggtcgcg gctccgacgc gtgtgatcga
     3661 ctgtacggtg ggcggggtct tgtcgatcgt gtagctctgg cctgacgtga aggctccgct
     3721 cgtggcgttg ccggcgctgt cggcggcgct gccgaggaag tcgagctgga gcgtgccgtc
     3781 gccgctgccg gtgttgacgg taaccgtgta cgtcgatccg ctgccggcga cattggcaat
     3841 cgtcgcgccc gagacgccgc tcatgaggag ggcgaagtcg ttggaggcaa caccggtgac
     3901 gctctcgctg aacgtgacgg tgaagtcgac gctcgatgca tgcgtcggag atgaattggc
     3961 gcgcacgatt gactgaacgg cgggcgcggt cttgtcgatc gtgtagacct cgcccgtggt
     4021 gaatgcggcg ccgagcgggg cgttgttgga ttcgctgtgg atcgatccgt cgttggcgac
     4081 gtcgaggcgg atcgtgccgt cgccggttcc ggtgccgacg ctgacggtgt agaccgagct
     4141 gttgactgga gagacgctga tgccgcccgg tgtgattcca cctgtcgtgg tcagcgtgaa
     4201 gtcgcctgca tcaacgccgg tgacgccctg cgagaaggtg accgtgaaat cgacggacgc
     4261 tgcgttcgtc gggttgctgc ttgcacgcgt caccgatagg acggccgggg cattggcatc
     4321 gacgaggacg ccggtggtgc tgccgacgga gttgagcgtg gtgttgccgt cgttgccgag
     4381 ggcgtcgcga atcgtggcgc cgttgaggtc gatgctgccg gcgaccacga tgccgtcagg
     4441 gtcggagtcg ccggagacga cggtgtagcg gaagagcagc gccgtgcctg gggagcccga
     4501 gacgtagttt gcgtacacgg ttccgctgtt gagcgtgagc gcgatgcgcg gcgtgccgct
     4561 ggccacggtg gcgttctcat tgaagttgac cgtgaagtcg aggttctgtc cggtcgtgta
     4621 ggtgatggct gccggtacgg cgaccgaggt gacattcggg ccgacgtgat cgagcgtgta
     4681 ggactggccg gtcgtgaatc caccgttgag cggattgccg ggaaggtcgg tgatgccggt
     4741 gccgctggca ttgacatcga gacgaagcgt cccctcgccc gcggcagaac cgacggtgac
     4801 ggtgtaggtg tgcgcgttga ccggaacgac tccggtgatg cttcccgagg ctccgccacc
     4861 ggccgtcagc gcaaacgcgc ccgtcgtcac cccgcttaca tcctcggaga acgtgacggt
     4921 gtaatgaagc gtgggggagt ttgtcggatc ggcgtcggcg cgaacgctgg tgaggacgat
     4981 cggggcgatg ccgtcgacga gaacgccagc ggtggaagcg aaggtcaccg cggtattggc
     5041 gttgttgccg gcaaagtctt tgatggtgct gccattgagc gcgataaagg aggctgtgga
     5101 cactccaccg ggggcggtgt cacctgagac gaccgtgtac cggaactgga gagttgccga
     5161 gccggaaccg gtgagatagg tcgccagtac ggggccacca tccagattta cggtaatcga
     5221 gggcgttcca ccagcgacga agacaggctc gctgaaggtc acgctgtagt cgagcgtctg
     5281 tccgatgatg tgcgtgccgt ttgcaggaac ggtcgaggag ctgacggtcg gcgcgacggc
     5341 gtcgacgaag acggcagacg tcgaagcgaa ggagacagag gtggccgcat tgttgcccgc
     5401 ggcgtccttg atcgttccgc cattcagcgt gatcgaggga tcgacgctaa tgccgtcggc
     5461 atcgaggttc cccgaggcta cgacatagcg gaaggtgagc gtggcggtgc cggtgccgga
     5521 aacgtacgca gcctgcacgg cgccgccagt gttcaacgtg acgccgacga acggcgagcc
     5581 ggtcttgatg accggctcgc tgtacgtgac cgtgaaatcg aggttctgac cggcgacgta
     5641 ggtgccgtca gcaggaacgg tcgaagaact gacggttgga gcgatggcgt cgaccttcac
     5701 tccggtggtg ctgcccacgc cgttcaaggt ggtgagcgcg tcgttgccgg cggcatcttt
     5761 gatcgtgccg ccgttcgcct cgaacgcggc accgaccacg attccgtccg gatcttcgtt
     5821 gcctgtcacg accgtatagc ggaagagcgc cgacgagccg ccgcccgaga cgtagctcgc
     5881 gtacacggtt ccgccggtgt tcagggtgat cgggatacgc ggcgttccgc caccggtggt
     5941 gacgttgacg gtctcgctga agttgacggt gaaatcgagg ttctggccgg cgacgtaggt
     6001 gccgttggca ggcacggaga ctgaggttac agctggaggc gtggtgtcga cgggctcgcc
     6061 aaccgtccag tcggagaaac tgctgacgcc cgccagcgcg gcggtgtgcg ttgccggtgt
     6121 aaccgtgccc gggaagaagg tcggcactcc gccgctcacg cgcgccagtt ggtagttggc
     6181 ctcgttcccc tgcacgtcac cggcaaggta ttggaacgtg aggtcgccgg tcgggccgtt
     6241 cgttgccgtc aacgtccagt aacgctgaat ggagttgccc gccgagacat tgggttgcgg
     6301 tgcctgaacg gcaaccaccg tcaagtcgag aggaaacgtg cccgcggtga cgtttgccgt
     6361 gactggcgaa tagccgttgg cggtgccggc atcgaagatc ttgctgctgg ccgccgagaa
     6421 cgtccgtttg agattgccga ttacgtagct ggagctcgaa ccgctggttg ttgacgcagt
     6481 gccagtaagc gccagggtgt tggcggcggt tacgatgttg ccggcgttga gcgccaacgt
     6541 gccgcttaaa gtcaggttac tcgccaacgt aacgttgatt ccggacccgt taaccgcaag
     6601 gttcgcgtac gtggggagga acgcagtccc tgacttgacg ctcagtccga cggcggcgta
     6661 gtccagcaac gtcttcgtgg ccgtaaccgt gttcgacgaa acggtcagat tgtcgattcg
     6721 aaaggtcccc gccgcgctga aagctgcgtg cgcaggtacc gtggcgcggt ctgcagcgct
     6781 cccggcaaac gggtaaatcc tgatggttac gctcggttga ttgttcgcgc ccgcgcccag
     6841 ggtgaatgtt tgcgaattgc ccgtgaaggt agcgctggtt acgagaccgg tcgacgttgt
     6901 tgtgaacgtc gttccgtcgg tgctgaagag aaccccaacg cggcccggac ccgtgcttga
     6961 cgcctggcag tcgaaactca cggtcaggcc ggagaaccca gtcgtgttga cgacaaactg
     7021 gtagtagaag ccggctgcgg ctcccggatc ggtggttgag gaatcccagc cagctgcagt
     7081 caaagcctcg ccagccgctg cgccgccatt gaatgtacct gcaccaccga cgccggatat
     7141 ggttacggtt ccgccgactt tcgtgagagc tccgctgccc gtgtttacgg actgttcgac
     7201 gaggttctcg aagaccgtcc gcgtggtgtt gttctcgaag tcgtagtaaa gaatcggtac
     7261 ctgagcttgc gcggccctgc tcatcatgaa tgtgcaaaag accgccgcga gaaccagagc
     7321 ggttctcctc acgatccctt cgccatagct gtgcggaact cgggtgaggc cgaaggcaag
     7381 aagccgcgct gcaacgttac gattcgcgtt catagactct cctggcatcg caccgttcgg
     7441 tcaggagagg gaaagtgtcc gggcagactc tccgatcagc gaagtcatcc cgcacaccac
     7501 gcaccgagga agttgacgag ctgaggaacc gagggtttcg agggctggct cggagagtac
     7561 gaataaacat gcgcgaacgc aacaaaaaag atgaaccggg catgaattgg cgggtgggag
     7621 acaaccgtag cgcttgctta acccacactt ctcacactca ttagattggt ccccttccca
     7681 tcgagcgcct tctcccccac gctttttgtg ggggagaagg tggcgaagcc ggatgagggg
     7741 gcgtcccaac caacacgaca tccatgcatt tgctgcggtt tctggcagtt cggac
//
LOCUS       DATLVW010000469         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_102898, whole genome shotgun sequence.
ACCESSION   DATLVW010000469 DATLVW010000000
VERSION     DATLVW010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102898"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..803)
                     /locus_tag="VNN25_14145"
     CDS             complement(276..803)
                     /locus_tag="VNN25_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92719.1"
                     /translation="MAERKSATTHTDAVQALVEETRAMRDRVPNLVIPSKGDRRRLAP
                     AASVPPAFVERTAVAVTNSAALVRGGGLDPARTRDLMDFSSAYLLWADELEATAEFVR
                     HSAIAARNQAGSDALTTFALARRLAKPPENADLAPHVEDMRRALGRTRKGKSKPAPEP
                     AAEPTSEDNDTTPAK"
     gene            complement(1408..2601)
                     /locus_tag="VNN25_14150"
     CDS             complement(1408..2601)
                     /locus_tag="VNN25_14150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXH92720.1"
                     /translation="MSLDIHSAVTLSAVLALLMGLSLRYVLRDYPATLWPSIRLWFFG
                     TFLQPLGWVLYGLRGEVPDFVAIVLSNAILSLAFAKLFQALRSFVSRPADAVLVYAPV
                     VAVGLAEIVFTYVFPSIRGRVVSVSPLIAFQFAACAATMLLGSPRRRSYVLTGVSFGL
                     SACVLVLRTLYEGLRSGTLPSAFIATPMQTAVFGVGTFLPAISTFGFVLLCNDRLNLE
                     LVRQASIDPLTGISNRRALGEECARVIAAARRRGHRLAVLLVDVDHFKRINDDYGHAS
                     GDSVLQMLVATLRSELRPEDSFGRLGGDEFVVVLPAVDDTAARVVAERLRQAVEKTDF
                     VIQGNPSRLQVSIGFALSGPGDDDLDALLRRADLAMYAAKRAGRNQIVGPLDQPAQQT
                     THADA"
     gene            2836..3330
                     /locus_tag="VNN25_14155"
     CDS             2836..3330
                     /locus_tag="VNN25_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92721.1"
                     /translation="MSDDHDPLAEIERLDRFFDGDEEGERPIDVLRDRGVSIPDERDL
                     DDGTLHARLWELINAMAATGVFVESTNHLSDRELYRYLVKDALREETILPTPGSGGGW
                     HLSPLGDSDEDTEIYLRYFADDKTREHWHRDFGVTLPPKGKPPFDRDRLLPVQDFAVD
                     EGIQ"
BASE COUNT          713 a         1006 c         1051 g          627 t
ORIGIN      
        1 cacgaagtgg catcgcgaaa cgtgagggtg atgacgctgt caccatgcca cttcgtggcg
       61 atgactgtca cgttctggtt cggcagaatg ccgcgaacgg atgctccttc tttcagttct
      121 tcaagacgag acatgaatag ggcagtctac cgcgggacac tcgcagtgaa cctagatcaa
      181 cgaaccgggt cggaatttct gggtgacgtg aaggccccaa agccaaccta actaatggat
      241 cagcttcaaa accccctcct cccctcccgc ccacctcatt tcgccggagt cgtatcgttg
      301 tcctcggacg ttggttcggc cgctggttcc ggcgccggct tcgacttccc ctttcgggtg
      361 cggccgaggg cgcggcgcat gtcttcgacg tggggggcga gatcggcgtt ctcgggaggt
      421 ttggccaggc gccgggccag ggcgaaggtc gtgagggcgt cgcttccggc ctggttccgg
      481 gctgcgatgg cactgtgacg gacgaactcc gctgttgctt cgagctcatc cgcccagagc
      541 aggtatgcgc tcgagaagtc catgaggtcg cgcgtcctgg ctggatcgag tcctcctccg
      601 cggacgagcg ccgcgctgtt tgtgaccgcg accgccgtcc gttccacaaa ggccggcggc
      661 accgaagcgg cgggagcgag ccgcctgcgg tcgcctttcg acggaatcac gaggttcggt
      721 acgcgatccc gcatcgcccg tgtttcctcg actaacgcct gcacggcatc cgtgtgagtc
      781 gttgctgatt ttcgttctgc catgatcctt tcctttcatc ttgaggacgt tcggccggga
      841 aaaaggttgc ttcgtgaccg agacaggcag aattaaggtt cgtaaaccac aagttggggt
      901 tcagccacct gaaattgagg ttggatgacc ttgaattgag gttgtactac cttggacttg
      961 agattggatg accttaagtt gaggttgagt aacctcgaac ttgaggttca gcaaccttaa
     1021 agccaaggtt cgataacctc aatttgagga ctcataacct tgaattgagg ttgagtaacc
     1081 ttaattcgaa gtctggcaac cttgaactta aggtcgagac accttaattt aagctcggat
     1141 cacctcaact tcaggttgaa caaccctgag ttgaggttgt gcaacctcac gatgacgtgg
     1201 ttgaacgatt ggccagggtg ccttctcatt caaaaccgat cacgggacac gtggaagcgc
     1261 tcctccgcgt gctccgcgtc tccacgtgac acgcctttcc gccagagagc cacttcctga
     1321 ctcgcctcgg acacagcggc gaagacaacg tcgatgaatc cgatcatcga ggtcactctg
     1381 aagcaggaac ttgtgcgaga ttgatcctca agcgtccgcg tgcgtagttt gctgcgccgg
     1441 ctgatcgagg ggaccaacga tctgattgcg tccggctcgc ttggcggcgt acatggccag
     1501 atccgcgcgg cggagcaaag cgtcgaggtc atcgtcgcca ggtcccgaca gggcgaatcc
     1561 gatggagacc tgcaggcggg acggattgcc ctggatcaca aaatccgttt tctcgaccgc
     1621 ctgccgcagc cgttccgcga ccacgcgcgc ggcggtgtca tcgacggcgg gcaacaccac
     1681 cacgaattcg tcgccgccaa gcctgccgaa ggaatcctcc gggcgaagct cggaccgcag
     1741 tgtggctacc agcatctgca gaacagagtc gcccgaggcg tgaccgtagt cgtcgttgat
     1801 gcgtttgaag tgatcgacgt ccacgagtag aaccgccagc cggtgtcctc ggcggcgcgc
     1861 ggcggcgatc acgcgagcgc actcctcgcc gagagcgcgc cggttgctga tgccggtgag
     1921 cggatcgatt gaggcctggc gtaccagctc gaggttcagc cggtcattgc acagcagtac
     1981 aaaaccgaac gtgctgatcg cgggaagaaa cgtcccgaca ccgaacaccg cggtctgcat
     2041 cggcgtggcg atgaatgcgg atggcagtgt tccgctgcgc agcccttcgt agagtgttcg
     2101 gagcacgagc acacacgccg acaacccaaa cgacacgccg gtgaggacgt aactgcgcct
     2161 tcgcggcgac ccgagcagca tcgtcgccgc gcaagccgcg aactggaagg cgatcagagg
     2221 cgagacactg accacgcgcc cgcgaatgct cggaaacaca tacgtaaaca cgatctcggc
     2281 cagcccgacg gctacgaccg gcgcgtaaac aaggaccgcg tcggctggac gcgagacaaa
     2341 cgagcgcaag gcctggaaca gtttcgcgaa ggcgagactc aggatcgcgt tggacagcac
     2401 gatcgcgacg aaatcgggaa cttcgccgcg caggccatac agaacccagc cgagtggctg
     2461 caggaaggtg ccgaagaacc agaggcggat gctcggccac agcgtcgcgg gatagtcgcg
     2521 cagaacataa cgcaggctga gccccatcag cagcgcgagc accgctgaga gtgtgactgc
     2581 actatgaatg tcgagactca cgatgcgctg cctcgatgtg ttggtcccag tgtagacgag
     2641 ggagcgaggg ttacgagggg aaggcgcggt acgaatcgcc gcgccggcgt tgttacgggc
     2701 gaatgggcat ccagtacgcg aagaggacgt cgcagcaact ccttttgccc tgccggatga
     2761 cgattctcca ctcggtagaa aaacttgact ctcttccaat cccgagggag cctcggtgcg
     2821 tgaggagatt cggcaatgtc tgacgaccac gatccgctcg ctgagatcga acggcttgac
     2881 cggtttttcg atggcgatga ggagggagaa cgcccgatcg acgtcctgcg cgaccgcggc
     2941 gtctcgattc cggacgagcg cgatctcgat gacggcacgc tgcatgcgag actctgggag
     3001 ctcatcaacg ccatggctgc gacgggagtg ttcgtcgaat cgacgaatca cctgagcgac
     3061 cgcgagctgt atcgctatct cgtcaaagac gcgctccgcg aggaaacgat tctccccact
     3121 cccggctccg gcggcggctg gcacctcagc ccactcggcg acagcgacga agacaccgag
     3181 atctacctcc gctacttcgc agacgacaaa acgcgcgagc actggcaccg cgacttcggc
     3241 gtaacccttc cgccgaaggg gaaaccaccg ttcgaccgcg atcgcctgct gccggtgcag
     3301 gattttgcgg tcgatgaagg gattcagtga ggcgaaggga atgagacaaa acgacgataa
     3361 cgggctcgtt gtgttcgaag gtcgtcagat ccgaaag
//
LOCUS       DATLVW010000470         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_556002, whole genome shotgun sequence.
ACCESSION   DATLVW010000470 DATLVW010000000
VERSION     DATLVW010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556002"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..767)
                     /locus_tag="VNN25_14160"
     CDS             complement(93..767)
                     /locus_tag="VNN25_14160"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXH92722.1"
                     /translation="MKFAIHGYGKMGRTIEKMATELGHQVVCILDVDRDEPLGDAEVV
                     IDFSHASALDGTLALACDNHLGLVIGTTGWNDRLDDVKQRVEQAGIGCVYASNFSPGA
                     NVVFSLAQRAGALVGRFPEYAAGMEERHHDQKKDAPSGTALRIAAAVKEGSDGKLAPP
                     IASSRVGTEPGLHTLFFDSPDDLIEISHRARNRDGFARGAVMAAQLLRGRKGLFRFEE
                     LAFAEG"
     gene            complement(764..2131)
                     /locus_tag="VNN25_14165"
     CDS             complement(764..2131)
                     /locus_tag="VNN25_14165"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXH92723.1"
                     /translation="MIVIKFGGTSVGDAERVANAIDIVAERRHLQPIVVVSALSGVTD
                     DLVAATNAARDGDGERVNEIIDRIRQRHEDVAMRLVQQKFDFFDTFVKRLDKQVEEIR
                     TILSGVALLHEVTARARDKIVSIGEKLSSVLFAYSMMMRSLPGEHVESEDVIWTNDKF
                     GGATPRMDLTAEAARKTLLPIVERNHIPVMGGFIGRTVDGATTTLGRNGSDYSAAIVG
                     AAVGAEEVQIWTDVDGLLTCDPRFVPTARVIDHLSHEEAAELASFGAKLHPRTLEPAI
                     EAGIPVRVLNTQNPASPGTLITREGPGQTSGPRSVARKRNVTLVHMTTNKMLGAHGFL
                     ARLFHVFEELKISVDLIATSEVSVTVTVDEQHDIDELQRRLRDLASVEIIDGQCIIAI
                     VGRNLLRDAQVGARVFEALRDVPMQMLSLGRSGLNLSIVIDDAASDRAIQRIHHALFE
                     QAVAV"
BASE COUNT          437 a          793 c          721 g          457 t
ORIGIN      
        1 cagggctctc aatctcttgt ctcttgtctc ttgtctctga tctctcgtct ctgattgtct
       61 ctgatcactg gacgcaacgc atccttccgt cttcatcctt cagcgaacgc cagctcttcg
      121 aaacggaaca accctttgcg ccctcgcaag agctgcgccg ccatgaccgc gccgcgggcg
      181 aagccgtcgc ggtttcgggc gcgatgcgag atctcgatga ggtcgtcggg cgaatcgaag
      241 aagagcgtgt gcaggccggg ctcggtgccg acgcgcgagg aggcgatcgg cggtgcaagc
      301 ttgccatcgc tcccttcctt caccgccgcg gcaatgcgca acgccgttcc gctcggcgcg
      361 tcttttttct gatcgtgatg acgctcttcc atacccgcgg cgtactccgg aaagcgcccg
      421 accagtgcgc cagcgcgttg cgccagcgag aagacaacat tggcgccggg cgagaagttg
      481 gacgcgtaga cgcagccgat tccggcctgt tccacccgct gcttcacgtc gtcgagccga
      541 tcgttccagc cggtcgtgcc gatgacgagg ccgaggtggt tgtcgcaggc gagggcgagc
      601 gttccgtcca acgcgctggc gtgcgagaag tcgatcacga cctcggcatc acccagcggt
      661 tcgtcgcgat cgacatcgag aatgcagacg acctgatgcc ccagctcggt cgccattttc
      721 tcgatcgtgc gccccatctt cccgtaaccg tgaatggcga acttcatacg gccaccgcct
      781 gctcgaagag agcgtgatgg attctctgga tggcgcggtc agacgcggcg tcgtcgatca
      841 cgatgctgag attcagtcct gatcgtccga gcgagagcat ctgcattggc acgtcgcgca
      901 gcgcctcgaa gacgcgcgcg ccaacttggg catcgcgcag gaggttgcgc ccgacgatgg
      961 cgatgatgca ctgcccgtcg atgatctcga cgctggccag atcgcgcagg cgccgttgca
     1021 gctcatcgat gtcgtgctgc tcgtcgaccg ttaccgtgac ggatacctcc gaggtggcga
     1081 tgagatcgac ggaaatcttc agctcttcga aaacgtgaaa cagcctcgcc agaaaaccgt
     1141 gcgcgccgag catcttgttc gtcgtcatgt gaacgagcgt tacgttgcgc ttgcgtgcga
     1201 ccgagcgcgg tccgctggtc tgccccgggc cttcgcgcgt gatcaacgtt cccggcgatg
     1261 caggattctg cgtattgaga acgcgcaccg gaatgcccgc ctcgatggcc ggctcgagcg
     1321 tgcgcggatg cagcttcgcg ccgaacgagg ccagctccgc tgcctcttcg tgcgaaaggt
     1381 gatcgatcac ccgcgccgtc ggcacaaagc gcggatcgca cgtgagcagg ccatcgacgt
     1441 cggtccagat ctgcacttcc tcggcaccaa cagcagcgcc aacgatggcc gcgctgtagt
     1501 cggagccgtt gcgcccgagg gtggtcgtgg cgccatcgac agtccgtccg atgaagccgc
     1561 ccatgaccgg gatgtgattc cgctccacga tcggcagcaa cgtcttccgc gccgcctcgg
     1621 cagtcagatc catccgcggc gtggcgcccc cgaacttgtc attcgtccag atcacgtcct
     1681 cggattcgac atgctcgccc ggcagcgaac gcatcatcat cgaatacgca aagagaacag
     1741 acgagagctt ctcgccgatc gacacgatct tgtcgcgcgc ccgcgccgtg acttcgtgca
     1801 gcagcgcaac gccgctgaga atcgtgcgga tctcctcgac ctgtttgtca agccgcttga
     1861 cgaaggtgtc gaagaaatca aacttctgct gcacgagccg catggccacg tcttcatgac
     1921 gctgtcggat acggtcgatg atctcgttga cacgctcccc gtcaccatcg cgcgccgcgt
     1981 tcgtggcagc aacgagatcg tccgtcaccc ccgaaagcgc ggaaacgacg acgatcggtt
     2041 gcagatgccg ccgctccgcc acgatgtcga tcgcattcgc cacccgctcc gcatcgccga
     2101 ccgaggtgcc gccgaacttg atgacgatca tggcaggccg ccagagtgat cagcgatcag
     2161 tgatgaagag atcagggagg ccggaaagcg agctgctgcc ttatcttgcg accctttccc
     2221 atcgagcgcc ccctcatccg gccttcggcc accttctccc ccactgaaaa gcgtggggga
     2281 gaaggggctc gattgaggtg tcgtgaggga aagttcattc ggtgactggc aggtggaggc
     2341 aaagcgaaga gctcgaactc tctcactcaa acctccttgc gccgccctcc aatcgagcgc
     2401 cttctccc
//
LOCUS       DATLVW010000471         4884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_390830, whole genome shotgun sequence.
ACCESSION   DATLVW010000471 DATLVW010000000
VERSION     DATLVW010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390830"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VNN25_14170"
     CDS             complement(<1..489)
                     /locus_tag="VNN25_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXH92724.1"
                     /translation="MFIPKSIVCLREGYTRERLFRDLLAGVVVGIVALPLALAFAIAS
                     GVPPERGLYTAVIAGFLISALGGSRVQIGGPTGAFVVIVYGIVAKYGYDGLVVITIMA
                     GVILVILGLARMGALIKFIPYPVITGFTSGIAVIIFSSQLKDFLGLKMGAIPAEFMEK
                     WTA"
     gene            complement(640..1386)
                     /locus_tag="VNN25_14175"
     CDS             complement(640..1386)
                     /locus_tag="VNN25_14175"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH92725.1"
                     /translation="MFETSVIRAEVVGERRVRMVTLSFAFHGLVVIAIFAASIRSIDF
                     PTSSPREYAIPIFALPPTIPPPLGTPNGGHKQATPVPAKEKAPAATANMAPNKLPEHV
                     TPAASTTPSDATTNSDSTGSDQPKGVPWGTKDSIGDGPPATNTAPATPDVPIPVTGDV
                     KAPVVIHRVSPLYPRLALSAHMNGTVVVECIIDKTGRIRDARVLTSSSPMFNQSAIEA
                     VEQWQFAPGTLYGNKIDTIFDLTVTFKVTM"
     gene            complement(1729..2823)
                     /locus_tag="VNN25_14180"
     CDS             complement(1729..2823)
                     /locus_tag="VNN25_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH92726.1"
                     /translation="MRHRYSYLDPDLIRQLLEEMGLRRLFNHLLLQSGGDVDEAMRWM
                     RALQDRGYIDPNIDLDAFFARLLEENVIGQDADGNVVLAAGGERSIRRDALDEIFGGL
                     QKSLTGSHSVNTAGQGSERLTETRPYQFGDDPMSIDSIRSLQNAMKHTDPADSILMAE
                     DDLEVFETEHNSSCATVVMIDISHSMILYGEDRITPAKKVALALTELILTKYPKDSID
                     VLLFGDEAKEVPISEIPYVQVGPFHTNTKAGLELAQRILRRHKHNNKQIFMITDGKPS
                     ALTENGHLYTNSFGLDMKIVNRTLEEADRCRRANIPITTFMLATDPMLVNFVEQLSKI
                     NRGRAFYSSPDRLGEYILADYIRNRRKFVH"
     gene            complement(2945..3364)
                     /locus_tag="VNN25_14185"
     CDS             complement(2945..3364)
                     /locus_tag="VNN25_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXH92727.1"
                     /translation="MAKVTVEIPNDLARQIDRIIRDGWFPDQEAIMREALSHFVDAKS
                     FLGDSPRMLHRFAADALNDSKPEVALKFVNRALSFLPTDEMTDFTLYQNLIELRVQIL
                     LVLDHTAEALTTLEEARETLPNNPTIAKWIERLKKRK"
     gene            complement(3390..>4884)
                     /locus_tag="VNN25_14190"
     CDS             complement(3390..>4884)
                     /locus_tag="VNN25_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH92728.1"
                     /translation="IARGESLFPGIHGYERTVIPAIQNAILSRHDIILLGLRGQAKTR
                     ILRSLTNFLDERIPIIAGCEINDSPLAPTCKRCRRLAAEQGDDLPIAWLTRDERYREK
                     LATPDVTVADLIGDIDPIKAANQRLTYADEEVIHYGIIPRTNRGIFAINELPDLAPRI
                     QVALLNILEERDLQIRGFPVRIPMDVVLVFSANPEDYTSRGNIITPLKDRIDSQILTH
                     YPQNIDTAMRITRQEAWTDREGAIEIDIPDYINTLVEQIAFVARGSELVDQSSGVSAR
                     LTIAAMELLQSNMERRAALTHDRNVFPRLSDLAMLLPAITGKVEMVYEGEQQGSEVVA
                     RKLIGEAVAKLFATKFPAVERAGVGGRNERNRSYTDLGDDELDDAFASHRGRKQKPPP
                     RQEEKPAAKSEPNYDLILAWFGEGHKITLSDEQPFAAYFAELKRVPKLEETVQKYMQA
                     SHDRELAFWMEMVLEGLHQSLRLAREDLDSTITFQEMMKFNVLRTVK"
BASE COUNT          926 a         1457 c         1507 g          994 t
ORIGIN      
        1 cgccgtccat ttctccatga actcggccgg aatcgctccc atcttcaacc cgagaaaatc
       61 cttgagctgg ctcgagaaaa tgatcaccgc gatccccgac gtgaagcccg tgatcaccgg
      121 gtacggaatg aacttgatga gcgcccccat ccgcgccaag ccgaggatga cgaggatgac
      181 gccggccatg atggtgatga cgaccagccc gtcgtaccca tatttcgcca cgatgccgta
      241 gacgatgacg acgaatgccc cggtcggccc gccgatctgc acgcggcttc cgccaagcgc
      301 ggagatgaga aagccggcga tgacggcggt gtaaagcccg cgctccggcg gcacacccga
      361 ggcgatggcg aaagcgagag cgagcggcaa cgccacgatc ccgacgacga caccggcaag
      421 cagatcgcga aacaaccgct cgcgcgtgta cccctcgcga agacagacga tggacttggg
      481 aatgaacaac gcggcgcgat tatatggtgc gggtgcggga cgtcgtggtg tcacacactg
      541 tgaatcgacg gccccctcat ccgccttcgg caccttctcc cccactgaaa agcgtggggg
      601 agaaggcgct cgaacttact gcatcgcgca gccgcgtacc tacatcgtca ccttgaacgt
      661 cacggtcaga tcgaagatgg tgtcgatctt gtttccgtag agcgttccgg gggcgaactg
      721 ccactgctcg acggcttcga tcgcgctctg attgaacatc ggcgacgagc tcgtgagcac
      781 gcgggcatcg cgtatccggc cggtcttgtc gatgatgcat tcgacgacga cggtgccgtt
      841 catatgtgcg gaaagcgcca gacgtggata cagcggactc acgcgatgaa tgactaccgg
      901 cgccttcaca tcgccggtga ctggaatcgg gacatcaggc gtcgcaggcg cagtattcgt
      961 cgctggtgga ccatcgccga tgctgtcttt cgttccccag gggacgccct tcggctgatc
     1021 ggagccggtg ctgtctgagt tcgttgtcgc atcactgggt gtcgtcgacg cggctggcgt
     1081 cacatgctcg ggcagcttgt tcggagccat gttcgccgtg gcagcaggag ccttctcctt
     1141 cgctggtacc ggcgtggcct gcttatggcc gccgttgggt gtgccgaggg gaggcggaat
     1201 cgtgggcggc aaggcgaaga tggggatcgc gtactcgcgc ggtgagctcg taggaaagtc
     1261 gatgctgcga atgctggccg cgaagatggc aatgacgacg agtccgtgaa acgcgaatga
     1321 caacgtcacc atcctcactc gcctctcgcc aaccacctcg gcacgtatga cagatgtctc
     1381 gaacatggtc ctgtcctcct gccagtgttc gagcagaagg acgcgcgatt cgaaaccgta
     1441 cgttgcaacg aagcagtgaa cgtgaggatg acgaaacggc ggttttacgt aggtcggact
     1501 ctctagtccg actgcacgtg atgtcggact ggagagtccg acctacgtaa aacctcatcg
     1561 gcaaagtgaa gttgcgcgcg gctaaatgca cgaacttccg ccggttcctg gtgtagtccg
     1621 ccaagatgcg ttacgtaggt cggactctcc agtccgactg cacgcgatgt cggactagag
     1681 agtccgacct acgtaaatcc tcatcggcga agcgaacgtt gcgcgccgct aatgcacgaa
     1741 ctttcgccgg ttgcgaatgt agtccgccag aatgtattcg cccaaccgat ccggcgacga
     1801 ataaaacgcc cggccccgat tgatcttcga gagctgctcg acgaaattca ccagcatcgg
     1861 atcggtggcc agcatgaacg tcgtgatcgg aatgtttgcg cggcggcagc ggtcggcttc
     1921 ttccagcgtt cgattgacga tcttcatatc gaggccgaac gagttcgtgt agagatgccc
     1981 attctccgtc agcgcggatg gtttgccgtc ggtgatcatg aagatctgct tgttgttgtg
     2041 cttgtgacgg cgaagaatgc gttgcgccag ttccagccca gctttcgtat tcgtgtgaaa
     2101 cggcccgacc tggacatacg ggatctcgga gatcggcact tccttcgcct cgtcgccgaa
     2161 gagcagcaca tcgatcgagt ccttcggata cttcgtcaga atcaactccg tcagcgccag
     2221 cgcgaccttc ttggccggcg tgatgcggtc ctcgccgtag agaatcatcg agtgggagat
     2281 gtcgatcatc acgaccgtgg cgcagctcga gttgtgctct gtctcaaaca cttccagatc
     2341 atcctcggcc atcaaaatcg aatcagccgg atcggtgtgt ttcatcgcat tctgcagcga
     2401 acggatcgaa tcgatcgaca tcgggtcgtc gccaaactgg tacggacgtg tctccgtcag
     2461 gcgctcgctc ccctgcccgg cggtgttcac ggaatgcgaa ccggtgagcg acttctgcaa
     2521 gcccccgaag atctcatcga gcgcatcgcg gcggatcgaa cgctcgccgc ccgctgccag
     2581 aacgacattc ccgtccgcat cctgtccgat cacgttctct tccagcagcc gcgcgaagaa
     2641 ggcatcgaga tcgatgttcg gatcgatgta gccgcggtcc tgcagcgcgc gcatccagcg
     2701 catcgcctca tcgacgtcgc caccgctctg cagcagcaga tggttgaaaa gccgccgcag
     2761 ccccatctcc tcgagaagct ggcggatgag atcggggtcg aggtaggagt agcggtggcg
     2821 catggggggg aagcagagat tagagacaga gagaccagag atcagtaacc cggtctttct
     2881 tatctctgac ctgatctctt gtctctgtat ctctgatctc tgtatctctg atctctgtct
     2941 ttactcactt ccttttcttc aacctctcga tccacttcgc gatcgtcgga ttgttcggca
     3001 gcgtttcgcg ggcttcttcg agcgtggtca gcgcctcggc ggtgtggtcg agcaccagca
     3061 ggatctggac gcgaagctcg atcaggtttt gatagagcgt gaagtcggtc atttcgtcgg
     3121 tgggtaggaa cgacagcgcg cgattgacga acttgagcgc gacctccggt ttggagtcgt
     3181 tgagcgcatc ggcggcgaag cggtgcagca tgcgggggga gtcgccgagg aaggacttgg
     3241 cgtcgacgaa gtgcgagagc gcctcgcgca tgatcgcttc ctgatcgggg aaccaaccgt
     3301 cgcggatgat gcggtcgatc tgccgcgcca agtcgttggg gatctcgacc gtgaccttcg
     3361 ccatgacgga attgtagtct ggagactggc tacttcacgg tccgcagcac attgaacttc
     3421 atcatttcct ggaacgtgat cgtcgaatcg agatcctcgc gggccagacg aagcgactgg
     3481 tgcagcccct ccagcaccat ctccatccag aaagccagct cgcgatcgtg gctggcctgc
     3541 atgtatttct gaacggtctc ctcgagcttc ggcacgcgct tcaattcggc aaagtaggcg
     3601 gcgaacggct gctcgtcgga cagcgtgatc ttgtggcctt cgccgaacca cgccaggatc
     3661 aggtcgtagt tcggctcgct cttcgccgcc ggcttttcct cctgacgtgg aggcggcttc
     3721 tgtttgcggc cgcggtggct ggcaaaggcg tcgtccaact cgtcgtcccc gaggtcggtg
     3781 tatgaccgat tgcgctcgtt gcgtccgccg acgcccgctc gctcgaccgc cgggaacttc
     3841 gtcgcgaaca acttcgccac cgcctcgccg atcagctttc gcgccacgac ctcggagccc
     3901 tgctgctccc cttcgtaaac catctccact ttgcctgtga tcgcgggcag aagcatggcc
     3961 agatcggaaa gccgcggaaa aacatttcga tcgtgcgtca gcgcagcgcg ccgctccatg
     4021 ttcgattgca gcagctccat cgccgcaatc gtcagacgcg cggagacgcc ggacgattga
     4081 tcgacgagct cagagccgcg ggcgacgaag gcgatctgct cgactagcgt gttgatgtag
     4141 tcggggatgt cgatctcgat cgcaccttcc cgatccgtcc acgcctcctg ccgcgtgatc
     4201 cgcatcgccg tgtcgatgtt ctgcgggtaa tgcgtcagga tctgcgagtc gatgcggtcc
     4261 ttgagcggtg tgatgatgtt gccgcggctc gtgtagtcct cggggttggc gctgaagacg
     4321 aggactacgt ccatcggaat gcgtaccgga aagccgcgga tctgaagatc acgctcctcg
     4381 aggatgttca gcagcgccac ctggatgcgc ggcgcgagat cgggcagctc gttgatcgcg
     4441 aagatcccgc ggttcgtgcg cggaatgatg ccgtagtgga tcacttcctc atccgcgtac
     4501 gtcagccgct ggttggccgc cttgatcgga tcgatgtcgc cgatgagatc cgcgacggtg
     4561 acgtcgggcg tagccagctt ttcgcgatag cgctcatcgc gcgtgagcca ggcgatcggc
     4621 agatcgtcgc cctgttccgc ggccagcctt cggcagcgtt tgcacgtcgg cgcaagcggc
     4681 gagtcgttga tctcgcatcc ggcgatgatg ggaatgcgct cgtcgaggaa gttcgtcagc
     4741 gagcggagga tgcgcgtttt ggcctgaccg cgaaggccca gaagaatgat gtcgtggcgc
     4801 gaaagaatcg cgttctggat ggcggggatg accgttcgct cgtagccgtg aatgcccgga
     4861 aagaggctct caccacgcgc gatc
//
LOCUS       DATLVW010000472         4121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_390868, whole genome shotgun sequence.
ACCESSION   DATLVW010000472 DATLVW010000000
VERSION     DATLVW010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390868"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VNN25_14195"
     CDS             complement(<1..448)
                     /locus_tag="VNN25_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXH92729.1"
                     /translation="MRLFITGATGYIGSAVAPLLRDHGHDVTALVRPETDAKALRDKG
                     VVIMAGDLASLPSLSDSLGSFDAFLHLAQDHSPDAAALNRTALDVFTAHQGFLIYTSG
                     VWVFGNTDNETVDESSPVNPLPIVAWRPEQEQAALATGRSAVLRPGC"
     gene            complement(448..1860)
                     /locus_tag="VNN25_14200"
     CDS             complement(448..1860)
                     /locus_tag="VNN25_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXH92730.1"
                     /translation="MTQSRESAEATFAEAFEASLNFKTPEQGELLKGQIVSISGDDAF
                     VSYGGPSEAVMATNELDGLDVGDNVEGTVVKTSPEIRISRKLAKGKANLDLLRQMYEN
                     RLPVEAKIAARNKGGFDVNISGLRAFCPLSQIALGKIDNPDQFIGQSYEFRVTELSDD
                     GRRIVVSRAALLKEANASRAEETRRNIVPGAELSGTIKTITPFGAFVDLGGIDGLLHV
                     SEMSRRRVTDPNEVVVVGQQVNVKVIKVEGEGKRISLSMKDQEPDPWSDVAERYPAGT
                     QFSGRIVRTTDFGLFVEVEPGIDGLVHYSQLPFGVKQGGAEVAIGTNVTGWVREVDPS
                     KKRLSLSLREVATRDPWEGAAQRYPAGKVVEGTVDHGGAPGIFVQIEPGLTGLIPNSE
                     INVAPGADPSRAHEAGEKLAVRIMSIDTQRKRISLSHEAAKAAAERDEYVKFAEERGD
                     NEAGESAMALAFKRAMEKKK"
     gene            2391..3062
                     /locus_tag="VNN25_14205"
     CDS             2391..3062
                     /locus_tag="VNN25_14205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXH92731.1"
                     /translation="MTTRVYLVRHGATELTDEDRFAGAIDVKLSDEGRDQARRLGLRL
                     QGEPISAAYASPMVRTMDTAALIVASHGLPVTPVDGIREIAHGRWERKTRAEVESEFP
                     EEYARYETDPFSFAPTGGESGLQVTARALPAFLAIVARHADQRILVVSHKATIRLLVS
                     SLIGVDPRKYRDRLDQSPCALNILDFKDVTCPRLTLFNDTSHYAGDVPDVPKNRLSKT
                     WGGRK"
     gene            complement(3141..>4121)
                     /locus_tag="VNN25_14210"
     CDS             complement(3141..>4121)
                     /locus_tag="VNN25_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92732.1"
                     /translation="DTTLVVNATDDLSGIRGISGTLTSPNGKAVQGFAQQRDGETTRY
                     VSRIHIPEKAEEGIWKITFLTMSDMATNSVTLSAGQGTLPPTAVLKVTSSASDSTAPT
                     LRNVWLERGAMRAGEHNTLYVDAQDDKSGVHSVTAVFLSPSKLARFGAGCHTGANSVW
                     ECDVSPPQCLDCGDWTLDQVNLQDNANNFAVIRSENPAVQQLRLNITGDTCDNQPPAL
                     QGLALSRTEIVMGRDEPVIEFVAQVSDDSCGMGGVSGQIAGPSGSSSGTFFTLQQRDA
                     TTFVGTVRLHPNSARGVWRMQSITVNDKGQNLKIYSSSDPLLANAIFRVK"
BASE COUNT          702 a         1242 c         1375 g          802 t
ORIGIN      
        1 cgcagccggg gcggagaacg gcgctgcgtc cggtggccag ggcggcctgc tcctgctcgg
       61 gacgccaggc cacgatcggg agcggattta cggggctcga ctcgtcgacg gtctcgttgt
      121 cggtgttgcc gaagacccag acgcctgatg tgtagatgag gaagccctgg tgcgcggtga
      181 agacatcgag ggcggtccga ttcagcgctg cggcatcggg actgtggtcc tgcgccaggt
      241 gcagaaaggc gtcgaagctg ccgagcgaat cggagagcga cggcagcgac gcgagatcgc
      301 cggccatgat gacgacgcct ttgtcgcgga gagccttcgc gtccgtttcc gggcgcacga
      361 gcgcggtcac gtcgtgtcca tgatcgcgaa gcagcggcgc gaccgcgctg ccgatgtatc
      421 ccgtggcgcc ggtgatgaag agccgcacta cttcttcttc tccatagccc gcttgaatgc
      481 cagggccatc gcgctctcgc ccgcttcgtt gtcgccccgc tcttcggcga acttgacgta
      541 ctcatcacgc tcggcggccg ctttggctgc ctcgtgcgaa agggagatgc gcttgcgctg
      601 cgtgtcgatg ctcatgatgc ggacagcgag cttctcgccg gcttcgtggg cgcgcgatgg
      661 atcggcgccg ggggcgacgt tgatctcgga attcgggatg aggccggtga ggcctggctc
      721 gatctggacg aagatgccgg gggcgccgcc gtgatcgacg gtaccttcga cgaccttgcc
      781 ggcgggatag cgctgtgcgg cgccttccca cggatcgcgg gtggctactt cgcgcagcga
      841 gagggagagg cgcttcttgg agggatcgac ttcgcgaacc cagcccgtca cattggtgcc
      901 gatggccact tccgcgccac cctgcttcac gccgaaggga agctgcgaat agtggacgag
      961 gccgtcgata ccgggctcga cctcgacgaa gaggccgaaa tcggtggtgc ggacgatgcg
     1021 gccgctgaac tgcgtaccgg ccggatagcg ctcggcgacg tcgctccagg gatcaggctc
     1081 ctgatctttc atcgagagcg agatgcgctt cccttcgcct tcgactttga tcaccttgac
     1141 gttgacttgc tggccgacga cgacgacttc gttcggatcg gtgacgcggc ggcggctcat
     1201 ctcggaaacg tggaggaggc cgtcgatgcc gccgaggtcg acgaaggcac cgaagggggt
     1261 gatggtcttg atcgtgccgc tgagctccgc gcccggaacg atgttgcgcc gggtctcttc
     1321 ggcgcgcgat gcgttcgctt ccttcaacag agcggcgcgg gagacgacga tgcggcggcc
     1381 gtcatcggag agctcggtga cgcgaaactc gtacgactgg ccgatgaact gatcggggtt
     1441 gtcgatcttg ccgagggcga tctgcgagag ggggcagaaa gcacgcagcc cgctgatgtt
     1501 cacgtcgaag ccacccttgt tgcgggcagc gatcttcgct tccacgggga ggcggttctc
     1561 gtacatctgc cggagcaggt cgagattggc ctttcccttg gcgagtttcc gcgagatgcg
     1621 gatctcgggg gaggttttaa cgaccgtgcc ttcaacgttg tcgccgacgt cgagcccatc
     1681 gagctcgttc gtcgccatca ccgcttcaga cggtccgccg taggaaacga aagcatcgtc
     1741 tccggagatg gagacgatct gtcctttgag caactcgccc tgctcggggg ttttgaaatt
     1801 caagctcgct tcgaacgctt cggcgaacgt tgcttcagcg ctttctctcg actgggtcat
     1861 ggatggagaa acctttcagt ggactggatc tcaggtctac gagatcggtt gggcagcgta
     1921 tcttactcga aagtggaatc gctggcgaca ccggaacccc ggacgtcccg catctcttcc
     1981 gtgcatcggt ttccggcctc atgggcgagc cgaaaaaaaa tctttgttac ccacttgccg
     2041 cgcgcgaaat gcggacctag attacagatc cctgacgcgc cggatgcttc atcgcagatg
     2101 gtaacggcgc gctgggtggt tccgagacgc gatgggtcga ggaactgctg cggggatgcc
     2161 actttgcggc tgtcaacgcg cagcaaacgg ttgcgacggg caaagaacgt cggagccagt
     2221 cggttcgaat gccccctggt ggctgtcaac ggatcgagaa cgggctcgcg agatcaggga
     2281 tcgcgagcct ttggtgtttc ttctctcaca cgaagtcgcg aagacgcgaa aaaggttcct
     2341 ctcccgcttc gcgtcttcgc gtcttcgcgt gaaacagtta cgatccgtcc atgacaacgc
     2401 gcgtctacct cgtccgccac ggcgccacgg agctgactga tgaagaccgc ttcgccggcg
     2461 caatcgatgt aaagctctcg gacgaaggac gggaccaggc acggcggctc ggcttgcgtc
     2521 ttcagggcga gccgatcagc gccgcgtacg ccagcccgat ggtgcggacg atggacacgg
     2581 ccgcgctgat cgtggcctcg cacggcctgc ccgtcacgcc ggtcgatgga atccgcgaga
     2641 tcgcgcatgg ccggtgggaa cgaaagacgc gtgccgaggt ggagtcggag ttcccggaag
     2701 aatacgcgcg gtatgagacc gacccctttt cgttcgcgcc gaccggcggc gaaagcggtt
     2761 tgcaggtgac cgcgcgcgcg ctcccggcgt ttctggcgat cgtcgccagg catgcggatc
     2821 agcgcattct cgtggtgtcg cacaaggcca cgatccgtct cctcgtcagc tcgctgatcg
     2881 gcgtcgatcc gcgcaagtat cgcgaccgtc tcgaccagag tccctgcgca ctcaatatcc
     2941 tcgacttcaa ggatgtcacc tgcccgcggc tcaccctgtt caacgacacg tcgcactacg
     3001 cgggcgacgt gcctgacgtg ccgaagaacc ggctctcgaa gacctggggc gggcggaagt
     3061 aattgacaat ggataatgga ttagcgcgga ggtagcggta tccacgctat caattgtcca
     3121 ttgtcagttg tcaattaccc tcacttcacc ctgaagatcg cgtttgccag caacggatcg
     3181 ctcgaggagt agatcttcag gttctgtccc ttgtcgttga cggtgatcga ctgcatgcgc
     3241 cacacgccgc gggccgagtt ggggtgaagg cggaccgttc ctacgaaggt ggttgcgtcg
     3301 cgctgctgga gcgtgaagaa ggtgccgctg gagcttccac tggggccggc gatctgtccg
     3361 ctgacgccgc ccatgccgca actgtcgtcg gagacctgcg ccacaaactc gatgaccggt
     3421 tcgtcgcgcc ccatcacgat ctcggtgcgg ctcagtgcca gtccctgcag cgcaggcggc
     3481 tggttgtcgc aggtatcgcc ggtgatgttg agccggagct gttgcacggc cggattctcc
     3541 gaacggatca cggcgaagtt gttggcgttg tcctgcaaat tcacctgatc gagcgtccag
     3601 tcgccgcaat cgaggcattg aggcggggag acatcgcact cccacacact gttcgccccc
     3661 gtgtgacagc cggcgccgaa gcgcgccagt ttcgacgggc tcaggaagac ggcggtgacg
     3721 gaatggacac ccgacttgtc gtcctgcgcg tcgacgtaga gcgtgttgtg ctcgccggcg
     3781 cgcatcgcgc cacgctcgag ccagacattg cgcagcgtcg gagcggtcga gtcggacgcc
     3841 gatgaggtca ctttcagcac ggctgtcggt ggcagagtcc cctgcccggc gctgagggtc
     3901 acggagttcg tggccatgtc gctcatggtc aggaaggtga tcttccagat gccttcttct
     3961 gctttctccg gaatgtggat gcggctgacg tagcgcgtgg tctctccgtc gcgttgctgc
     4021 gcgaaaccct gcaccgcttt gccgttcggg ctggtcagcg tgccggagat cccgcgaatg
     4081 ccggagaggt cgtccgtggc attaacgacg agagtggtgt c
//
LOCUS       DATLVW010000473         3057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_473782, whole genome shotgun sequence.
ACCESSION   DATLVW010000473 DATLVW010000000
VERSION     DATLVW010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473782"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..913)
                     /locus_tag="VNN25_14215"
     CDS             complement(14..913)
                     /locus_tag="VNN25_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXH92733.1"
                     /translation="MDSGAVTPGNAIEHEALPQPPHEDLPKRHPLAKFLSGAAITIGV
                     VLIKLKALLVVVFDFFRGYAVNPFEGFGLMQFSVAGGTMALSIWAMATKAPLPLVVGF
                     VGIILVHEIGHAVAIRAKGLHAGLMVFIPFIGGAVTLKDQPRSAYDDAQIGLAGPVAG
                     TFASLIVLQVYKWNEQPLYLAIAAAGFAVNLINLLPIGMLDGGRISAAVTKWMWVFGG
                     GVLIYKVVKQPNPLMLIILVLAAFQVYLSIMREKDDKSFYDVTLSQRVFVAVAYFGLV
                     IFLGHQTYMSLNRLAALSAPLVK"
     gene            962..2611
                     /locus_tag="VNN25_14220"
     CDS             962..2611
                     /locus_tag="VNN25_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH92734.1"
                     /translation="MPVPSVDDLRRELRDRGYLSHGIERWFAHDPFTAGRSSRAFWRE
                     LTTVAGKASLLIALFAIVPLVAIMVFRNHPLSAGETLLLSLLYGLTGFAVSFALLILV
                     ALVLKLRPAIAIDTPRALLAISFAASAIPSGLIAIWWYGFDAPPSWPELLLGLALIVA
                     LFLFSSVAVSAALLSFSIYELQRIPAIHRKPRTIPMTMAAIFLIAVLFLPAYASQDKG
                     AAEPPLQVVTTPSTRRIALIAVDGLTADILRSRPDLVTALPATYAVAPLRGGSTTERW
                     ASLGTGVKTVLHGVRSVEGVRLWNGGHLLQSVSQRDIVLRGLGGAVGLAKREPLPPTV
                     RRRDFVWEIFAARGVPSVAVNWWTADDAHAGALDSIGQMPVFAAAASGGGAPEMIALR
                     VDEISIKRLLHAADADKPRFATVYLPALDVILNRLAIDPSARLTASVHALDGVVAAVE
                     ALRARGFDVIVVGMPGDHQAGQAVLTSTFALAPTTTPFDVAPTLCALIGFPPSNEMSG
                     RTLAGSEPPRIASYGSRVSRQQATKMNEEYYENLRSLGYIR"
     gene            2722..>3057
                     /locus_tag="VNN25_14225"
     CDS             2722..>3057
                     /locus_tag="VNN25_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH92735.1"
                     /translation="MRRFTFTLVLLALASTVAAQRTMTFEDLASIHRIGGPQLSPDGQ
                     WIAYDVSTPDLKANKSFSGVYIMPSAGGAPKLISDVKHQDNGPAWSPDGKTIAYVSNR
                     DGGAHQVYIY"
BASE COUNT          542 a          979 c          952 g          584 t
ORIGIN      
        1 ttgagcctcg tccttacttc accaacggtg ccgacagcgc cgccagacgg ttcagcgaca
       61 tgtaggtctg gtggccgagg aagatgacga ggccgaagta ggcgacggcg acgaagacgc
      121 gctgggaaag cgtcacgtca tagaacgact tgtcgtcctt ctcgcgcatg atcgacaggt
      181 acacctggaa ggcggccagg acgaggatga tcagcatcag cgggttcggc tgcttgacga
      241 ccttgtagat cagcacgccg ccgccgaaaa cccacatcca tttggtaact gcggcggaga
      301 tgcggccgcc atcgagcatc ccgatcggca gaagattgat caggttcact gcgaaccctg
      361 cagcggcgat ggcgagatag agcggctgct cgttccactt gtaaacctgc agaacgatga
      421 gcgacgcgaa ggtgccggcc acggggccag ccagcccgat ctgggcgtcg tcgtacgcgg
      481 aacgcggctg atccttcaac gtgacggcgc cgccgatgaa cgggatgaac accatcaacc
      541 cggcgtgaag gcctttcgcc cggatggcaa cggcgtggcc gatctcatgg acgaggatga
      601 tgccgacgaa tccgaccacg agcggcagcg gtgctttcgt cgccatcgcc cagatcgaga
      661 gagccatcgt tccgccggcg acggaaaact gcatgaggcc gaagccttcg aacgggttga
      721 cggcataccc gcgaaagaaa tcgaagacca ccacgaggag cgccttcagt ttgatgagga
      781 caacgccgat ggtgatggcg gcgccggaga ggaatttcgc cagcggatgg cgtttcggca
      841 ggtcttcatg cggcggctgg ggcagcgcct catgttcgat cgcattgcca ggggtaactg
      901 cgccggaatc cattgaagag tcgggcgata gaacgatcgg cccggtgcta tattccgcgc
      961 cgtgccggtg ccgtccgtcg acgatttgcg tcgcgagttg cgggatcgcg ggtacctctc
     1021 tcacggcatc gagcggtggt tcgcacacga tccctttacc gccggacgct cctcccgtgc
     1081 gttctggcgg gagctgacca cggtcgcggg caaagcctct ctcctcatcg ccttgttcgc
     1141 gatcgtccct ctcgtcgcca tcatggtttt tcgcaaccat ccgctgtccg cgggcgagac
     1201 tctcctgctg tcccttctct atggactgac aggattcgcg gtttccttcg cccttctcat
     1261 tttggtggct ctcgttctca agctccggcc ggcgattgcg atcgatacgc cgcgggcgct
     1321 gctggccatc tcctttgctg caagcgccat cccttccggc ctgatcgcga tctggtggta
     1381 cggcttcgac gcgccgccgt cgtggccgga gctgctgctg ggactggcgc tgatcgtggc
     1441 gctcttcctc ttcagcagcg tcgccgtctc cgccgcccta ctgtcgtttt cgatttacga
     1501 gctgcaacgg attcctgcca ttcaccggaa accacggacg atcccgatga cgatggccgc
     1561 catcttcctc atcgcggttc tcttccttcc ggcgtacgcc agtcaggaca aaggtgcggc
     1621 cgagccgccg ctacaagtcg tcacgacgcc gagcactcgc cgcatcgcgc tgatcgccgt
     1681 cgacggcctg accgcggaca tcctccgctc tcgccccgac ctggtcacgg cgcttcccgc
     1741 gacttacgcc gtcgcaccgc tgcgcggcgg ttccactacg gagcggtggg cctcgctcgg
     1801 taccggcgtg aagactgttc tgcacggcgt acgttcggtt gaaggcgtca ggctatggaa
     1861 cggtggtcat ctgctgcaat cggtgtcaca gcgcgacatc gtgctccgcg gtctgggcgg
     1921 cgcggtgggg ctggcgaagc gggagccgct tccgccgacg gtgcgccggc gcgatttcgt
     1981 ctgggagatt ttcgcggccc gaggagtgcc gagcgtcgca gtgaactggt ggacggctga
     2041 cgatgcgcac gccggcgcac tcgactccat cggccaaatg ccggtcttcg ccgcggcagc
     2101 atccggcggc ggagctccgg agatgatcgc gcttcgtgtc gatgaaatat cgatcaagcg
     2161 gcttttgcac gcagccgatg ctgacaagcc gcgctttgcc acggtttatc tccctgcgct
     2221 cgacgtgatt ctcaaccggc tggccatcga tccgtcggcc aggttgacag cgtccgttca
     2281 cgcactcgac ggggtggttg ccgcggtcga agcactgcgt gcgcgcggct tcgatgtgat
     2341 cgtcgtcgga atgcccggcg atcatcaggc cggtcaggcg gtgcttacgt cgacgttcgc
     2401 gctggcaccg accacgaccc cgttcgatgt cgccccgacc ctctgcgcac tcatcggctt
     2461 cccaccatcg aacgagatgt ccggccgcac gctcgccggc agcgaaccgc cgcgcatcgc
     2521 cagttacggt tcacgcgtct cgcggcagca agcaacgaaa atgaatgagg agtattacga
     2581 gaatctgcgg tcgctcggat acatacgatg aaaagcagaa ggatgaaggc agaaggcaga
     2641 aggatgagga acgacccggc ttagcgctcc ttctgccttc tgccttctgc cttcatcctt
     2701 tcaaacacgg aggtttcaac catgaggcgc ttcacattca ccctggtact tctcgccctg
     2761 gcctcgacgg tcgcggcgca acgcacgatg acgtttgagg atctcgcgtc gattcatcgc
     2821 atcggaggtc cgcagctctc gccggacggc cagtggatcg cgtatgacgt ctccacgccg
     2881 gatctcaagg cgaacaagag cttcagcggg gtctacatca tgccgtcggc gggcggcgca
     2941 ccaaagttga tcagtgacgt caaacatcag gacaacgggc cggcctggtc gccggacgga
     3001 aagaccatcg cctacgtctc gaaccgcgac ggcggcgcgc atcaggttta catctac
//
LOCUS       DATLVW010000474         8292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_556128, whole genome shotgun sequence.
ACCESSION   DATLVW010000474 DATLVW010000000
VERSION     DATLVW010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556128"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VNN25_14230"
     CDS             <1..384
                     /locus_tag="VNN25_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXH92736.1"
                     /translation="EKNASDELVHLLQIWILPERHGITPSYEQKVFPPAERDGRLRLV
                     ASHDGRDGSVTIHQDADLYTTTLRDNATVTQDLRPGRYGWVQVARGTVTVNGKPLTAG
                     DGAAIEAEPRVTIAGDGEVLLFDLA"
     gene            473..1120
                     /gene="nth"
                     /locus_tag="VNN25_14235"
     CDS             473..1120
                     /gene="nth"
                     /locus_tag="VNN25_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXH92737.1"
                     /translation="MTKKEKASRIGTILDDLFPAPPIPLEHADPFTLLVAVVLSAQTT
                     DVQVNKVTPWLFARASTPKKLAAMSEAEILALIRSCGLAPGKARNIRKLAQTLVDEHG
                     GKVPRDMDALEKLPGVGHKTASVVMSQAFGEPAFAVDTHIHRLAARWGLSNGKSVEQT
                     ERDLKKVFPRDEWSRRHLQIIYFGRQYCPARNHDQSACPICSWAAKSNRSRTRSR"
     gene            1125..3224
                     /locus_tag="VNN25_14240"
     CDS             1125..3224
                     /locus_tag="VNN25_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXH92738.1"
                     /translation="MRTIVLSFLIAALGPFALARPVARPAVRADDAGQFDVRPAPAWA
                     DVLHPDLHAPQRIVRSGIDGLLEDHQIRVVGANVDEYFRDVRKVLTSAGVQNASEISI
                     DFDPSYERLVIHDVGLIRDGKRIDELTREDVRIIEKESESEDRIYDGQLTALLFLKDV
                     RAGDVIDYSWSLEGSNPLLGGRYAGEFDFSATVPTRLIRHRLIWPASRPLHTSKPATV
                     EHRGETDVYVWQRRDVPATDTEDSTPDWYDAFETLQVSEYGSWSEVAKWADGLFRIDD
                     ASRAAVTALANEISATHPDREGRIVAAIRFVQDDIRYLGIEMGRGSHEPRQPSVVLAQ
                     RYGDCKDKALLLAALLRQLGVQAWPALVNTKLRQRLDEYLPSPFLFDHVITEVADGGK
                     IYWIDGTLSDQGGTLTTIDTPSDERAFVVRPDSNGLTPIVIRTHGTIAVEEVISPADA
                     SGHAANPSHILAVTSTYTARDADELRSKLASESLADVAKDHLNRYAADHPHIEALGAP
                     SIDDDRLRNIIVLRERYTIRDLWKTGSWTYYPRAVEKHLDRPTTLVRSTPLAIEYPLD
                     VTERLIVRGGASPKIQEHDIVVGSPALHYEQHVEQGPDLIVITTLRSTKDALPVAKVP
                     DHLAALNEMRDRLDITLEPTHATVTPLAATIATLALVGLATSLSVRRKRRSVVVPAEV
                     IESPSETYRSDRSDRTA"
     gene            3348..3557
                     /locus_tag="VNN25_14245"
     CDS             3348..3557
                     /locus_tag="VNN25_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXH92739.1"
                     /translation="MRSTGTVKWFNDAKGFGFITSASGEDVFVHFSAIQSKGFRSLAE
                     GAQVEFDMVQGPKGLQAANVTVEGQ"
     gene            complement(3612..4655)
                     /locus_tag="VNN25_14250"
     CDS             complement(3612..4655)
                     /locus_tag="VNN25_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92740.1"
                     /translation="MPWKPIALVTTTASILLLLLLLPACCCVKGPLAPISGPPKIETP
                     LIGTEPIPTPTEAAAKAPTQTMMRNVWFHIDQDSYLDIHSLRGEMVSKTPGAPLNFDN
                     KLAFILKVDTGTIGMRSASLDVLMNRYIFGYPNAPLRNLHIEMAGKQLKQTGIIHKIV
                     DIPFTMFADVSASNGKIRIHPTKIDICGINGAGLLKAVGMTLQKMLKMPEERGIKAEG
                     NDLLLDPQRALPPPQVELHLVSVRVEGDELIQVFDAGLHLPELTLPHPEEKNTMYYRG
                     GTLRMGKLLMVDADMQVVDSDPSDPFDFDIDHYNDQLVAGFVRNQPNYGLFVFMRDFS
                     DLGKPARPGERMH"
     gene            4992..5738
                     /locus_tag="VNN25_14255"
     CDS             4992..5738
                     /locus_tag="VNN25_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92741.1"
                     /translation="MDYGIEVSGLTKRYDSLVAVKDLSFAVKRGEVLGLVGPNGAGKT
                     TTLRALAGIHPPSDGTIRICGFDLRNDAVDAKRHLAFMPDEPRLFDYLTVEEHLQFVG
                     RLYQVTGVREKMDPLLNEFELTDKRKALPAELSRGMKQKLMIACGFIHEPDVLIFDEP
                     LTGLDPLGIRRMKESIVRRSQAGASVILSSHLLHLVEELCDTVAVIQNGTRIAYDTID
                     NLKFALAKGRSDLSLEEAFLRITAEETPAT"
     gene            5735..7477
                     /locus_tag="VNN25_14260"
     CDS             5735..7477
                     /locus_tag="VNN25_14260"
                     /inference="COORDINATES: protein motif:HMM:NF028272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HXH92742.1"
                     /translation="MIGTFATVALLTFKNRILQRLRKLREPRYLIGAVAGAAYFWFFF
                     FRRAHNSGVALARIRDLSFNAVIVDFASVVVLFMMIAAWALPADSGGLDFSEAEIAFL
                     FPAPLRRRDLLLYKILRAQPQALFSAVMLTIIGTRQGYFIGTWAAFSVMAVYFTMVSQ
                     GRARLRQFHISFLPRLGVVALIIGGLYALVLKQIHTIRPIAIPANYQQTIKAGGSARA
                     AIEAFFQKPVIAAILFVPKFFASAALPGSLTTLVDSVLVVAALGVIFFFIASALNVSF
                     EEASISTSQRRAVRRERQHSQRIGSFVMFRRAPALFKLAETGPVEVAIIWKNLIALMR
                     NSITWVVILAATMALLLGLAMWTHDASGYLMIGTGLLVLAGFFPLLGPNMFANDLRLD
                     MPRLEVLKSYPISGERLVAAEIAAPLAVISILEMMFASSASVLMAMAEQTNRWMRMVA
                     TPQFIVTVLLLTIPVCAIQLLIRNAVPVLFPGWAVRSKDEPRGFVMTGQRLVTLAGNI
                     LVLVVSLLPAAIVFAPSLWVAYKFFSGNAAFVAVATMPAVAVLVAEFWFGVKALGAQF
                     ESLDVSNEMDVMAT"
     gene            complement(7481..8137)
                     /locus_tag="VNN25_14265"
     CDS             complement(7481..8137)
                     /locus_tag="VNN25_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HXH92743.1"
                     /translation="MSLTRDQIVRRAAEELRDGFYVNLGIGMPTLVANNIPEGMDVIL
                     QSENGILGLGPFPADADVDPDLINAGKQTVTVLPGASFFSSADSFGMIRGGKVDLSIL
                     GAMQVSAQGDIANWMVPGSMVKGMGGAMDLVASAKRLIVTMEHVTKKGEKKILDACNL
                     PLTGVKCVDRIITDYCVIDVTAEGLKLIEVAPGVSADEVQKLTEPRLLIGSDLKEIRV
                     "
     gene            complement(8141..>8292)
                     /locus_tag="VNN25_14270"
     CDS             complement(8141..>8292)
                     /locus_tag="VNN25_14270"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXH92744.1"
                     /translation="GKICIAEVEELVEPGEIDPDCVHTPGIFVHRVVVAKRVKRIEQR
                     TVRKG"
BASE COUNT         1531 a         2746 c         2441 g         1574 t
ORIGIN      
        1 gagaagaatg catcggacga gctcgtgcat cttctccaga tctggattct tcccgagcgg
       61 cacggcatca cccccagcta cgagcagaag gtgtttccgc ccgctgagcg cgacggacgt
      121 ctgcgtctcg tcgcatcgca cgacggacgc gacgggtcag tgaccattca ccaggacgcc
      181 gatctctaca cgacgacgct tcgcgacaac gccacggtaa cgcaggatct tcgtccaggc
      241 cgctacgggt gggtgcaggt cgcgcgagga acggtgaccg tgaacggcaa gccgctcacc
      301 gccggtgacg gcgccgcgat cgaggccgag ccgcgggtaa cgatcgcggg cgatggggaa
      361 gtgctgctct tcgatctggc gtaggtgtta aaccacagag acacaaaggt cacagaggtt
      421 acgatgcact ctctgtgtcc tcagtgtctc tgtggttgct gtttttcgaa ccatgacgaa
      481 gaaggaaaaa gcatcacgca tcggaacgat cctcgacgat ctcttccccg cccctcccat
      541 ccccctcgag cacgccgatc ccttcacgct gctcgtcgcc gtcgttctct ctgcgcagac
      601 taccgacgtg caggtcaaca aagtcacgcc ctggcttttc gcgagagctt cgacgcccaa
      661 gaaactcgcg gcgatgagcg aagcagaaat cctggcgctg ataagaagtt gcggactagc
      721 tcccggcaaa gcgcgcaaca tccgcaagct cgcgcagacg ctggtcgatg agcacggagg
      781 caaggtcccg cgagatatgg acgcgctcga aaaactgccc ggcgtcggcc acaagaccgc
      841 cagcgttgtc atgtcgcagg ccttcggtga gcccgccttc gcggtcgata cgcacatcca
      901 tcggctcgcc gcgcgttggg ggctgtcgaa cggaaagagc gtcgagcaaa ccgagcgcga
      961 cctcaagaaa gtctttcctc gggacgaatg gagccgccgc cacttgcaga tcatctactt
     1021 cggccgccag tactgtcccg ctcgcaatca cgatcagagc gcgtgtccca tttgttcgtg
     1081 ggcagcgaaa tccaatcgct cccgtacgcg ttcacgctga tcacttgcgc acgatcgtcc
     1141 tttcgttcct gattgcggca ctcggcccgt tcgcacttgc ccggccggtt gcccgtcccg
     1201 ccgtccgcgc cgacgacgcc ggccagttcg acgtccgtcc cgctccggcc tgggccgacg
     1261 tgctccatcc cgatctccat gccccgcagc gcatcgtccg gtccggcatc gacgggctgc
     1321 tcgaagacca tcagattcgc gtggtcggcg ccaacgtcga cgagtacttc cgcgatgtgc
     1381 gcaaagtgct cacctcggcc ggcgtgcaga acgcgtcgga gatcagcatc gacttcgatc
     1441 cttcgtacga gcgcctcgtg atccacgacg tcggcctcat ccgtgacggc aagcggatcg
     1501 acgagctgac gcgcgaagac gtgcgcatca tcgagaaaga gtcggaatcg gaagaccgca
     1561 tttacgacgg tcagctcacg gcactcctct tcctcaaaga cgtgcgcgcc ggcgatgtca
     1621 tcgattactc ctggtcgctt gaaggctcga accctctcct cggcgggcgc tacgccggcg
     1681 aattcgactt cagcgccacc gttccgacac ggctcatccg ccaccgtctg atctggcccg
     1741 cgtcgcgccc gttgcacacc agcaagccgg ccaccgtcga gcatcgcggc gaaaccgatg
     1801 tctacgtctg gcagcggcgc gacgtaccgg cgacggacac cgaggattcg acgccggatt
     1861 ggtacgacgc gtttgaaacg ctgcaggtct ccgagtacgg atcgtggagc gaagtcgcga
     1921 agtgggccga cgggctcttt agaatcgacg atgcttcccg cgccgcggtg acagcgctcg
     1981 cgaacgagat ctcggccaca caccccgacc gcgagggccg catcgtcgcc gccatccgct
     2041 tcgttcagga cgacatccgc tacctcggca tcgagatggg ccgcggctcg cacgaaccgc
     2101 ggcagccatc cgtcgtcctc gcccagcgct acggcgactg caaagacaaa gcgctcctgc
     2161 tcgcggcgct ccttcgtcaa ctcggcgttc aggcctggcc ggcgctcgtc aataccaagc
     2221 tgcgtcaacg gctcgacgaa tacctaccgt cgcccttcct tttcgatcac gtgatcacgg
     2281 aggttgccga cggcggcaag atctattgga tcgacggcac gctctccgat cagggcggaa
     2341 cgcttaccac gatcgacacg ccgagcgacg agcgcgcgtt cgtcgttcgg cccgattcaa
     2401 acgggctgac gccgatcgtg attcgcacgc atggcaccat cgcggtggag gaagtgatct
     2461 cgcccgcgga cgcatcgggc cacgccgcga atccatcaca catccttgca gtcacgtcga
     2521 cctacacagc ccgcgacgcc gacgagcttc gctccaagct tgcgtcggaa tcgctcgccg
     2581 acgtggccaa ggatcacctc aaccgctacg ccgccgacca cccgcacatc gaagcacttg
     2641 gcgcgccgtc gatcgacgac gaccgcctcc gcaacatcat tgtcctgcgc gagcgctaca
     2701 ccatccgcga cctctggaag accggctcgt ggacctacta cccgcgcgcc gtggaaaagc
     2761 atctcgaccg tccgacgacg ctcgtccgct ccacccctct ggcgatcgag tacccgctcg
     2821 atgtcaccga gcgcctgatt gtccgcggcg gcgcgagccc caagatccag gagcacgaca
     2881 tcgtcgtcgg cagtccggcg ctgcactacg agcaacacgt cgagcagggc cccgatctga
     2941 tcgtaatcac gaccttgcgc tcgacaaagg atgcactgcc ggtcgcgaag gtccccgatc
     3001 acctcgcggc actgaacgaa atgcgcgacc gcctcgacat cacccttgag ccaacccacg
     3061 ccacggtcac cccgctggcc gctacgatcg ccaccctcgc cctcgtcgga ctggcgacgt
     3121 cactgtccgt gcgaagaaag cgccggagcg tggttgtccc tgccgaggtg atcgagtcgc
     3181 ccagcgaaac atatcggtca gatcggtcgg atcggacggc gtaagcgttc aacgctcagc
     3241 cattcgccgg acggaaatag gtatccataa ccgaatgttg gtccgggctt ggcgcagcaa
     3301 gtggctgcgc tggacaaaga agaaaccaac cagaggagat tctcagaatg cgtagcaccg
     3361 gtacggtcaa gtggttcaac gatgcgaagg gctttggctt catcacgtcg gccagcggcg
     3421 aggacgtctt cgtgcacttt tctgcgatcc agtcgaaggg cttccgttcg ctcgccgagg
     3481 gcgcgcaggt ggagttcgac atggtgcagg gtccgaaggg actgcaggca gccaacgtca
     3541 cagtcgaagg ccagtaacgt tcgcacagac aacccctgga aggacggcct gcgagccgtc
     3601 cttttttcgt ttcagtgcat ccgctcgccg ggccgcgccg gtttgccgag gtcggagaaa
     3661 tcgcgcatga agacgaagag gccgtagttc ggctgattgc gaacgaaacc ggccacgagc
     3721 tggtcgttgt aatggtcgat atcgaaatcg aacggatcgc tcggatcgga atcgacgacc
     3781 tgcatgtcgg catcgaccat cagcagtttc cccatccgta gcgtgccgcc gcggtagtac
     3841 atcgtgttct tctcctccgg atgcggcaag gtcagctcag gcagatggag gccggcatcg
     3901 aagacctgaa tcaactcatc gccctcgacg cggacgctga cgagatgcaa ctccacctgc
     3961 ggcggcggca aggcgcgctg cggatcgagc agcagatcgt ttccctccgc cttgatcccg
     4021 cgctcctccg gcatcttcaa catcttctgg agcgtcatgc cgaccgcttt gagcaagccg
     4081 gctccattga tcccgcagat gtcgatcttc gtcggatgga tgcgaatctt tccattagac
     4141 gccgagacgt ccgcgaacat ggtgaacggg atgtcgacga tcttgtgaat gatgccggtc
     4201 tgcttcaact gctttccggc catctcgatg tgcagattgc gcagcggcgc attcgggtac
     4261 ccgaagatgt agcggttcat cagcacgtcg aggctggcgc tgcgcatgcc gatcgtgccg
     4321 gtatcgacct tcaggatgaa ggcgagcttg ttgtcgaagt tcagcggcgc gcccggcgtc
     4381 ttcgagacca tctcgccgcg caacgaatga atatcgagat acgagtcctg atcgatgtga
     4441 aaccagacgt tgcgcatcat cgtctgcgtc ggcgccttcg ccgccgcctc ggtgggcgta
     4501 ggaatcggct cggtgccgat gagcggcgtc tcgatcttcg gcggcccgct gatcggcgcc
     4561 aacggcccct tcacgcaaca gcacgccggt agaaggagca gcaggagaag gatggacgcc
     4621 gtagtcgtga cgagcgcaat cggcttccaa ggcacagagc gagacatatg caaggctgag
     4681 ctttcacaaa gggtgccggg tggtgcgggg gggttgggtg tagggagtgg gggggaggac
     4741 gggtcagcga ttcggaaggg gtcagacccg aagtggaagt ggtttccgga aggggtcaga
     4801 ccggaagtgg aagtgacgga agatggcgct ggcaggaagc tcagacaaat cgcgattgaa
     4861 ctgtcacttc cacttcgggt ctgccccctt ccgtgtcatc gcgcagattg ctgatctccc
     4921 accccccact ccccaccccc cactccccgc tccgaaaacc gacacccgcg aaaaccgtat
     4981 gattcccaca catggactac ggcatcgagg tctccggcct caccaaacgt tacgactcgc
     5041 tcgtggcggt gaaggatctc tccttcgccg tgaagcgcgg cgaggtgctc gggctcgttg
     5101 ggccgaacgg cgcggggaag acgaccacgc tccgcgcgtt ggcgggcatc cacccgcctt
     5161 ccgacggcac gattcgcatc tgcggcttcg atctgcgcaa cgatgccgtc gacgccaagc
     5221 gccacctcgc cttcatgcct gacgagccac ggctcttcga ttacctcacc gtcgaagagc
     5281 acctgcagtt cgtcgggcgg ttgtatcagg tcaccggcgt gcgcgagaaa atggatccgc
     5341 ttctgaacga attcgagctt accgacaaac ggaaggcgct tcctgccgag ctctcgcgcg
     5401 gtatgaaaca gaagctgatg atcgcgtgcg gattcattca cgaacccgac gttctgatct
     5461 tcgatgagcc gctcaccggt ctcgatcccc tcggcatccg ccgcatgaag gaatcgatcg
     5521 tccggcggtc gcaggccggc gcgtcggtca tcctcagctc gcatctgctg catctcgtcg
     5581 aggagctctg cgacaccgtg gccgtgatcc agaacggcac gcggatcgct tacgacacca
     5641 tcgacaatct gaagttcgcg ctcgccaaag gccggagcga tctctcgctc gaggaagcgt
     5701 tcctgaggat caccgccgag gagacgccgg caacgtgatc gggacgtttg ccactgttgc
     5761 gctcctcacg ttcaagaacc gcatcctgca gcgactgcgg aagctccgcg agccgcgcta
     5821 cctgatcggg gccgtcgccg gcgccgcgta cttctggttc ttctttttcc ggcgcgcgca
     5881 caacagcggc gtcgccctcg cccgaatccg tgatctttcg ttcaacgcgg tcatcgtcga
     5941 cttcgccagc gtcgtcgtgc tgttcatgat gattgccgcg tgggcgctgc cggccgactc
     6001 cggcggtctc gacttcagcg aggcggaaat cgcctttctc tttcctgccc cactgcgccg
     6061 ccgcgatcta ctgctctaca agatcctccg cgcgcagcca caagcactct tcagcgccgt
     6121 gatgctgacg atcatcggca cgcggcaggg atacttcatc ggcacctggg ccgccttctc
     6181 cgtgatggcg gtctacttca cgatggtctc gcaaggccgc gcgcgcctcc gtcagtttca
     6241 catcagcttt ctcccgcgcc tcggcgtcgt cgcgctgatc atcggcggcc tctacgcgct
     6301 cgtactgaaa cagatccaca ccatcagacc gatcgccatt ccggccaatt accaacagac
     6361 gatcaaggcg ggaggctccg cgcgtgcggc gatcgaagcg ttctttcaaa agccggtcat
     6421 tgccgcgatt ctcttcgtgc cgaaattctt cgcgtctgct gcgctgccgg gatcgctgac
     6481 cacgctcgtg gactcggtgc tggtcgtcgc tgcgctcggc gttatcttct tcttcatcgc
     6541 ttcagcgctc aatgtctcct tcgaggaggc atccatcagc acatcgcagc gccgagcggt
     6601 ccgccgggag cggcagcaca gtcagcgcat cggctcgttc gtcatgtttc gccgcgcgcc
     6661 tgcgctcttc aaactcgcgg agaccgggcc ggtcgaagtg gcgatcatct ggaagaatct
     6721 catcgccctg atgcgcaact cgataacctg ggtcgtcatc ctcgcggcga ctatggcctt
     6781 gttgctgggt ctggcgatgt ggacgcacga cgcgtccggg tacctgatga tcggcacagg
     6841 actgctcgtc ctcgccggtt tctttcccct ccttgggccg aacatgttcg cgaacgatct
     6901 ccgactcgac atgccgcgcc tcgaggtatt gaaaagttac ccgatcagcg gcgagcgcct
     6961 cgtggccgcg gagatcgccg caccgctggc cgtcatctcg attctggaaa tgatgttcgc
     7021 gtcgtccgcg tccgtcctga tggcgatggc cgagcaaacc aaccgctgga tgcgcatggt
     7081 cgcaacgcct cagttcatcg tcaccgtcct tctgttgacg attcccgtct gcgccattca
     7141 gctcctcatc cgcaacgctg tgcccgtgct cttccccggc tgggccgttc gcagcaagga
     7201 cgaaccgcgc ggctttgtca tgaccggcca gcgcctggta acactcgccg gcaacatcct
     7261 cgtccttgtc gtatcgctac ttcccgcagc gatcgtcttc gcgccatcgc tctgggtcgc
     7321 ctacaaattc ttctccggca acgccgcctt cgtcgccgtc gccacgatgc ccgcggtagc
     7381 cgtcctcgtc gctgaattct ggttcggcgt caaagccctc ggcgcccaat tcgagagcct
     7441 cgatgtgtcg aatgagatgg atgtgatggc gacgtaggcg tcagacgcgg atttctttca
     7501 agtcgctgcc gatgagcagc cgcggctccg tcagcttctg aacctcatcg gcgctcacac
     7561 cgggcgccac ctcgatcagc ttcaatccct cggccgtcac atcgatcacg caatagtcag
     7621 tgatgatgcg atcgacgcac ttcacgccgg tgagcggaag gttgcaggcg tcgaggatct
     7681 tcttctctcc ctttttcgtg acgtgctcca tcgtcacgat gaggcgcttg gcgcttgcga
     7741 cgaggtccat cgcgccgccc atccctttca ccatcgagcc gggcaccatc cagttggcga
     7801 tatcgccttg tgcagagacc tgcattgccc cgaggatgga gagatcgact ttgccgccgc
     7861 ggatcatgcc gaaggaatcg gcgctggaga agaaggatgc tccgggtaac acggtaaccg
     7921 tctgtttgcc cgcgttgatg agatcgggat cgacgtcggc gtcggcgggg aatggcccga
     7981 ggccgaggat gccgttctcg gattgaagga tgacgtccat gccctcgggg atgttgttcg
     8041 ccacaagcgt gggcattccg atgccgaggt tgacatagaa accgtcgcgc agctcttcgg
     8101 ccgcgcggcg aacgatctga tctcttgtta atgacatcgg ttaccccttc cgcacagtgc
     8161 gctgttcgat gcgcttgacg cgcttcgcca cgacgacgcg gtgcacgaaa atccccggcg
     8221 tgtggacgca atcaggatcg atctcgcccg gctcgaccaa ctcctccacc tcggcgatac
     8281 aaatcttccc tg
//
LOCUS       DATLVW010000475         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_230023, whole genome shotgun sequence.
ACCESSION   DATLVW010000475 DATLVW010000000
VERSION     DATLVW010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230023"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..493
                     /locus_tag="VNN25_14275"
     CDS             107..493
                     /locus_tag="VNN25_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92745.1"
                     /translation="MRQRLEIKRSNSNHDNRLLSAIRNYLPGAWIAPKNNELITLYRL
                     RYKMALEERKYDAALIFLNKILELDPLNAEAKLCKGEIYHRCLQDYAKAVEQYNKVLR
                     ISGGRDSESIQLRARAGMAEIMELLS"
     gene            complement(724..1740)
                     /locus_tag="VNN25_14280"
     CDS             complement(724..1740)
                     /locus_tag="VNN25_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXH92746.1"
                     /translation="MTSPPISSAPVEQLSRADHLLDLVAPKLQLVELELKRNFESGIK
                     TLHDVGEHILAGGGKRLRPALLLLVAKMLRYEGHRDVTYGAVVEFIHNATLVHDDIID
                     EAEVRRGRTSINYRWGNNLTVLVGDYIFMHSMSMALAEGNLDVLRLLSTTTIKMIEGE
                     ILALEHNGRADVSVDDYFTIVERKTALLFGACCRIPAYLVDQPEAIGQSLFSYGVNLG
                     ICFQLIDDILDFTSSTEVLGKPALSDLKEGKLTLPLILAMPRATPAEREKIALIAAMK
                     SFEGIDPAEMLALVRKYETLDETRAIARDYANRARTALEPFADSPAREALEMALDFVL
                     ERDR"
     gene            complement(1773..2267)
                     /locus_tag="VNN25_14285"
     CDS             complement(1773..2267)
                     /locus_tag="VNN25_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HXH92747.1"
                     /translation="MAQEFSFDVVSKTEMQEVSNAVQQAQKELAQRFDFKGSKSSIEL
                     AAEEIVLASDDEGKLRSVKDILESKLVKRGVSLKAMDYDVLEQAMGGTVRQRAKIVQG
                     IEIEKAKAIVKAIKEAKLKVQASIQSDQVRVTGRTKDDLQKAIALVKGKDFGIPLQFT
                     NYRG"
BASE COUNT          493 a          750 c          763 g          514 t
ORIGIN      
        1 ctcgaaaacc aactccacaa aacaatagtc aacctcttga cgatcgccgt ttccgaaact
       61 aagatgatgt cattcgagtg acacagatta cgggagggtg tgagtgatgc gacagcggct
      121 ggagatcaag cgctcgaatt ccaatcacga caatcgtctt ctgtcggcga ttcgcaacta
      181 cctgccgggt gcctggatcg cgccgaagaa caacgagctg atcacgctct accggctccg
      241 ctacaaaatg gcactcgaag agcggaagta cgacgcagcg ctcatcttcc tgaacaagat
      301 cctcgagctc gatccgctca acgccgaggc taagctctgc aagggcgaga tctaccatcg
      361 ctgcctgcag gactacgcga aggcggtaga gcagtacaac aaagtcctgc gcatctccgg
      421 cggacgggac agcgagagca tccagctacg cgcccgtgcg ggcatggccg agatcatgga
      481 actgctctcg tagctggctt gcacagacag gagtgtctgt gccacatcac ttctggcttc
      541 gaggcgctga ttcggcgact tggggctctt ccaggaaatc caagtcactt caggcttcga
      601 gttgcaccgg atcgaagtcc cggggttgtg tggcactgac actcttgtct gtgctagcaa
      661 tgcctcatga gtactttcta tgataaagta ctcaccatgt caagccggac gacaccgact
      721 cgttcagcgg tcccgctcga gcacgaagtc gagcgccatc tccagcgctt cccgtgccgg
      781 cgagtcggcg aacggttcga gagcggtacg cgcgcggttc gcgtagtcgc gggcgatggc
      841 gcgcgtctcg tcgagcgtct cgtacttgcg caccagcgcc agcatctcgg cggggtcgat
      901 cccttcgaac gacttcatcg cggcgatgag tgcgatcttc tcgcgctccg ccggggtggc
      961 ccgaggcatg gccaggatca agggcagggt cagcttgcct tccttgagat cggagagcgc
     1021 cggctttccg aggacttctg tgctcgaggt gaagtcgaga atgtcgtcga tgagctggaa
     1081 gcagataccc aggttgacgc cgtacgaaaa gagcgactgg ccgatcgctt ccggctgatc
     1141 gacgagatag gcagggatgc ggcagcaagc gccgaacagc agcgccgtct tccgctcgac
     1201 gatcgtgaag tagtcgtcga ccgagacatc ggcgcgtccg ttgtgctcga gggcgaggat
     1261 ctccccctcg atcatcttga tggtcgtggt cgacagcagc cgcagcacgt caaggttgcc
     1321 ctcagcaagc gccatcgaca tggagtgcat gaagatgtag tcgccgacca ggaccgtgag
     1381 gttgttcccc cagcgatagt tgatcgaagt ccgcccgcgc cggacttcgg cctcgtcgat
     1441 aatgtcgtcg tgaacgagcg tggcgttgtg gatgaattcg acgaccgctc cgtacgtgac
     1501 gtctcgatgg ccttcgtagc gaagcatttt cgcgacgagc agcagcaggg cgggccgtaa
     1561 ccgcttgccg ccgccggcga gaatgtgctc gccgacgtcg tgcagggtct tgatgcccga
     1621 ctcgaagttg cgcttcaact cgagctcgac gagctggagc ttcggcgcga ccaggtcgag
     1681 gagatggtct gcgcgcgaga gctgctcgac cggcgcgctg ctgattgggg gcgacgtcac
     1741 gggggacaga ataaacgaat ggtcaggaag cctcaaccgc ggtagttggt gaactgcagc
     1801 gggatcccga aatcctttcc cttcacgagg gcaatggctt tctggagatc gtctttcgtg
     1861 cggccggtga cgcggacctg atcgctctga atcgaggcct gaaccttgag cttcgcctcc
     1921 ttgatcgcct tcacgatggc cttcgctttc tcgatctcga tcccctgcac gatcttggct
     1981 cgctgccgca ccgtgccgcc catcgcttgc tcgagcacgt cgtaatccat cgctttgagc
     2041 gagacgccgc gcttcaccag tttcgattcg aggatgtcct taacgctgcg cagcttgccc
     2101 tcgtcatcgc tggcgagcac gatctcttcg gcagcaagct cgatcgagct cttgctccct
     2161 ttgaagtcga atcgctgggc cagctctttc tgcgcctgct gcaccgcatt cgaaacttcc
     2221 tgcatttcgg ttttggaaac gacgtcgaat gagaattcct gagccaaggg acctcctggg
     2281 tggggcgtgg ggagtagggg gtggggggtc ggagaaaaag ccggttcgtt gccaaagacg
     2341 gtatgaacgg ttgctctctc cgacccccga cccccgactc cccactccct attgttcaac
     2401 cacctccaca ccggccggcg gtgtgaagtg gaaaaagtcg gatgctacgc tgcgtttcgt
     2461 gaagccggag aaatcgaacg tcgtttcatt cccttcgcgg tccgagtaag ccacgcgctg
//
LOCUS       DATLVW010000476         6008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_103184, whole genome shotgun sequence.
ACCESSION   DATLVW010000476 DATLVW010000000
VERSION     DATLVW010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103184"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNN25_14290"
     CDS             <1..492
                     /locus_tag="VNN25_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH92748.1"
                     /translation="EELNEAADEAVKRFAFIDGTRMAAGGASYGGHLANWLEATTTRY
                     KCLVSHAGEINLESQWGTSDGIYHRELTNLGPVWEQGKVWREQNPIRYAAKFHTPMLL
                     SVGEHDFRVPMNETLENWSVLQRRRIPSKLLVWPDENHWILNPEDSRHWYGELWDWLA
                     KWM"
     gene            483..2651
                     /locus_tag="VNN25_14295"
     CDS             483..2651
                     /locus_tag="VNN25_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92749.1"
                     /translation="MDVAKRAGREAAIVCAYLLVAIAATWPLAARLDTAVSDLGDPLL
                     NAWIIDWDCYALTHAPQHIFDAPIFYPSRYPLAYSENLIGIALVVLPFHLAGLPPLAV
                     YNLAMLLGIAISAYAGYLLARVVTGKTAASAIAGLLYGFVPYKLGHLAHVQIIWSATP
                     ALLLAALILYRRKPSKKYAALVAIAFAASATINIYLFLFGAVALALTLVLIAIAERRN
                     RRFWLTLIVALAIASLAILPVLLPYAIVAKEYDMRRGDGETLGASAMPADWLVPSGNS
                     AVYGNLVPDAWHHSERELFPGLLLIALTIAAFVRRGAVAEVEEEPRRYRFRWLDALIA
                     LLVIATAVGLVLHSSTIPMTLLVIAIIARLPLRAWIGHSRFPVELWIAALWIVVGVIG
                     SFGLHAFFHSIFFHDVPGFRATRAPARWALIGYAGLAAASAVALARMRRRTLIVLALL
                     AAVDVWPRIRWEHALSEPTPFERWLAATHAGPLIELPLRNDLEYRYLLGAASSHVALF
                     NGISGFEPPLHRRLRTEPLGDTMFDLLEKNGCRFVLIHIDSFSPLPGIAFNWIRRGLD
                     EGRLVFVRRFDHEMNGDWLFALRRGPRTVPNEELQRLLDGKPTYNNATFGRIDRPRYN
                     ATFTGPLDVSGWALSPAGIREVDVLIDSGRVRIVAARTPNAQISGMYPWYRETTLPSF
                     AAHIEHRPPGVRDKTDVQVAIVDGAGRVTRMPDLLITWRE"
     gene            complement(2884..3147)
                     /locus_tag="VNN25_14300"
     CDS             complement(2884..3147)
                     /locus_tag="VNN25_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92750.1"
                     /translation="MISDPIPTARDVINHLNERFLAHGLPYRLDQITVLPYVSPLWMA
                     NWSVPQLDDAPERDIIDQEIAEARWKWPQVRDEEWELNPPVAT"
     gene            complement(3150..4517)
                     /locus_tag="VNN25_14305"
     CDS             complement(3150..4517)
                     /locus_tag="VNN25_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92751.1"
                     /translation="MKRLLIVEDKESLALMLRETVEAEGLEADVALDGKEAVRKLAAG
                     NRYFAVLTDLRMPGADGIAVLRQVKESDPDCPVIVMTAFATIENAVEAMKLGAYDFVQ
                     KPVDADHLLLLLRRCRENRELRYENILLKDEFRQRYRLPAIVGESARIVEVSNAIQRV
                     APTDSTVLLQGESGTGKELFARAIHQLSPRRDRPFVAINCAAIPETLIENELFGHEKG
                     SFTGATGRQLGKFELADSGTIFLDEIGELGLGVQSKILRVIQERRFDRIGGTSTLEVD
                     VRIICATNRPLAGEVKEGRFREDLFFRINVFPVTVPPLRARREDIDGLADFYLQKFAR
                     ELKKPSLRIAADAAETLRAYDWPGNIRELENCIERAAILCDGGVIERRDLHIEAPSTD
                     ALRDALDLSGTLEEATARTARLVERLKIAEALEQTNSRADAAEKLGISVRTLAAKMKE
                     HGIEE"
     gene            complement(4523..4876)
                     /locus_tag="VNN25_14310"
     CDS             complement(4523..4876)
                     /locus_tag="VNN25_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92752.1"
                     /translation="MDLADLVHAILTGDLLAARQWVADSQRARIEWERYEKPAGLDER
                     EMIVAAGLAELLAGRAGIPAPPWTTSVGPQDGLLVLDPGLELMPRSFEQAKRNGPEPL
                     RRRNMIASPDFLDIR"
     gene            complement(4913..>6008)
                     /locus_tag="VNN25_14315"
     CDS             complement(4913..>6008)
                     /locus_tag="VNN25_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXH92753.1"
                     /translation="FDYDVRTALTTDAVLAAVIGLALQDTLGNLFGGIAMHMEGGFNV
                     GDVLHSGDYVGVVESVSWRATRIRGFNNQAIVLPNSVIARERLEVFPLGRLTGRVLPV
                     SVDYNIPPATVIGILSQVAAHVDGVARDLPCFARVASFADSAVIYEIKYHTRDYSERD
                     RIDADIRKGVWYALHRNKIAFATPIRAYQQYMPPDVQHAISDDDVRSRLSEVDVLSPL
                     SEEARATIAAATRIHFYSKGETILRHGAAGDSMFVVHAGTASVRLPDDSPEGWHEVAQ
                     LGPGTVFGEMALLTGETRTADVVALTDVTALEIGKDSLQPILHGHPELASAITAKVVH
                     RRGHLDAIRSEASEEEETTIVSRVKAWFGL"
BASE COUNT         1030 a         1942 c         1901 g         1135 t
ORIGIN      
        1 gaggagctca acgaggcggc ggacgaagcg gtcaaacgtt tcgcgttcat cgacggcaca
       61 cgcatggccg cgggcggtgc gagttacggc ggtcacctcg cgaactggct ggaagcgacg
      121 acgacgcgct acaagtgcct cgtcagccac gccggcgaga tcaatctgga gtcgcagtgg
      181 ggcacgagcg acggcatcta tcaccgcgag ctcacgaacc tcgggcccgt ctgggagcag
      241 ggcaaagtgt ggcgcgagca gaacccgatc cgctacgcgg caaagttcca cacgccgatg
      301 ctcctctccg tgggcgagca cgatttccgc gtgccgatga acgagacgct cgagaattgg
      361 tcggtcctgc agcgccgccg catccccagc aaactgctgg tgtggccgga tgagaaccac
      421 tggatactca atcccgagga cagccggcac tggtacggcg agctgtggga ttggctggcg
      481 aagtggatgt agccaagcgc gccggccggg aggccgcgat cgtttgtgca tacctcctcg
      541 tggccatcgc ggccacgtgg ccgctcgccg cgcgtctcga taccgcggtc tccgacctcg
      601 gcgatccgct gctgaacgca tggatcatcg attgggattg ctacgcgctg acgcatgcac
      661 cgcagcacat tttcgacgcg ccgattttct atccttcgag gtacccgctg gcctacagcg
      721 agaacctcat cgggatcgca ctggtggtct tgccgtttca ccttgccggg ctgccgccgc
      781 tcgcggtcta caacctcgcc atgcttctcg gcatcgccat ctccgcgtat gccgggtacc
      841 ttctcgcgcg cgttgttacg gggaagaccg cagcatcggc aatcgccggc ctgctgtacg
      901 gcttcgtccc ctacaaactc gggcatctcg cgcacgtgca gatcatctgg agcgcaacgc
      961 cggcgctgct gctcgcggcg ttgatcctgt accgacgtaa accttcgaag aaatacgcag
     1021 cgctggtggc gatcgcattc gcggcaagcg ccaccatcaa catctatttg ttccttttcg
     1081 gcgccgtggc gctggcgttg acgcttgtat tgatcgcgat cgcggaacgg cgcaaccggc
     1141 gcttctggct gacgctcatc gtggcgctgg cgatcgcgag cctcgccatt ttgccggtgc
     1201 tgctgccgta cgcgatcgtg gcgaaggagt acgacatgcg ccgcggcgac ggcgagacgc
     1261 tcggcgcctc cgcgatgccg gccgactggc tcgtgccatc tgggaacagc gcggtttacg
     1321 ggaacctcgt tcccgacgcc tggcatcaca gcgagcggga gctgtttccg ggtctgctgc
     1381 tgatcgcgct gacgatcgcg gcgttcgtgc gcagaggtgc ggttgccgag gttgaggagg
     1441 agccgcgaag gtatcgcttt cgctggctcg acgcgctgat cgctctcctc gtcatcgcca
     1501 ccgcggttgg gctcgtgctt cactcatcga cgattccgat gactctgctc gtcatcgcaa
     1561 tcatcgcgcg cctgcctttg cgggcatgga tcggccactc gcgctttccg gtcgaattgt
     1621 ggatcgccgc gctctggatc gtcgtcggcg tgatcggctc tttcggactg cacgcgttct
     1681 ttcactcgat cttctttcac gacgtccccg gtttccgcgc cacgcgcgcg cccgcgcgat
     1741 gggcgctcat cggatacgcc gggttggcag ccgcgtcggc ggtggcgctc gcgcgcatgc
     1801 gacggcggac gctgatcgtg ctcgcgctgc ttgcggccgt cgacgtctgg ccgcgcattc
     1861 gctgggagca tgcgttgagt gagccgactc ccttcgaacg ctggcttgcc gcaacgcatg
     1921 ccgggcctct catcgaactg ccgctccgca acgatctcga atatcgctat ctcctcggcg
     1981 ccgcgtcttc ccacgttgcg ttgttcaacg gcatctccgg tttcgagccg ccgctgcacc
     2041 gccgcctgcg caccgaaccg ctcggcgaca ccatgttcga tctgctggag aagaatggat
     2101 gccgcttcgt cctcatccac atcgattcct tcagcccact tcccggcatc gccttcaact
     2161 ggatccggcg cgggctcgat gaagggagac tggtcttcgt ccgccgcttc gatcacgaaa
     2221 tgaatggcga ttggctcttc gcgctgcgac gcggcccgcg caccgtgccc aacgaagagc
     2281 tgcagcgtct tctcgacggt aaaccgacgt acaacaacgc aacgttcgga cgaatcgacc
     2341 ggccacgtta caacgcgacg ttcaccggtc cgctcgacgt atcgggatgg gctctgtcgc
     2401 cagcgggtat tcgcgaagtc gacgttctca tcgatagtgg acgcgtgcgc atcgtggcag
     2461 cgcgaactcc caacgcgcag atcagcggga tgtatccgtg gtatcgcgag acgacgctcc
     2521 cctcgttcgc ggcgcacatc gaacatcgtc ctcccggcgt gcgggataag acggacgttc
     2581 aggttgcgat cgtcgacggt gcgggacgcg tgacgcgcat gccggacctg ctgatcactt
     2641 ggcgggagta gttacgcggc catttcgacc tccgccctta cagcactcgg gcgaggacgc
     2701 ccgagtgccc gcggtcgaga cgaccgcact ccttacgacg cggactgagg agtgtgggcg
     2761 tctcgcccgc ggccatcggg gcgtctcgcc tcgatggttg gatcgggcgg atagctccaa
     2821 tgatttgata agggtcaacc gaagacaaga cttgccggag acgagatcga aagcagtctc
     2881 cattcaggtg gctaccggag ggttgagctc ccactcttca tcacgaactt gcggccactt
     2941 ccaacgcgct tcagcgatct cctgatcgat gatgtcgcgc tcgggcgcgt cgtcgagctg
     3001 tggcaccgac cagttggcca tccacaacgg cgagacgtag ggaagcacgg tgatctgatc
     3061 gagccgatag ggcaggccgt gcgcgaggaa gcgctcgttg aggtgattga tgacgtcgcg
     3121 cgcggtgggg atagggtctg agatcaccgt cattcctcga tgccgtgctc tttcatcttc
     3181 gcggctagcg tgcggacgga aatgcccagc ttttctgctg catccgcgcg cgaattcgtt
     3241 tgctccagcg cctcggcgat cttcagccgc tccacgaggc gtgccgtacg cgcggtggct
     3301 tcctccaacg tcccggagag atcgagggca tcgcggagcg cgtcagtcga aggggcttcg
     3361 atgtgaagat cgcgacgctc gatcacgccg ccgtcgcaga ggatggccgc ccgttcgatg
     3421 caattctcca actcccggat gttgcccggc cagtcgtacg cgcgcaatgt ctcggcagca
     3481 tcggcagcaa tccgcagcga gggcttcttt agctcgcggg cgaacttctg caaatagaaa
     3541 tcggcgagcc catcgatgtc ctcgcgccgc gctcgcaacg gcggcacggt gaccgggaag
     3601 acgttgatgc ggaagaagag gtcttcgcgg aagcggcctt cctttacctc gcccgcaagg
     3661 gggcggttcg tggcgcagat gatgcggaca tcgacttcga gcgtgctcgt gcctccgatg
     3721 cggtcgaagc gccgctcctg aatcacacgc aggatcttcg actgcacgcc gagaccgagc
     3781 tcgccgatct catcgaggaa aatcgttccg ctgtcggcca gttcgaactt gccaagctgc
     3841 cgtccggtgg cgccggtgaa cgaacctttc tcgtgtccga aaagctcgtt ctcgatgagc
     3901 gtctccggaa tggcggcgca gttgatggcg acgaacgggc ggtcgcggcg cggcgagagc
     3961 tgatggatgg cacgggcgaa gagctccttc cccgttcccg actccccctg cagcaacacg
     4021 gtcgaatcgg tcggtgccac gcgctgaatg gcgttggata cttcgacgat gcgcgccgac
     4081 tcgccgacga tcgccgggag gcggtagcgc tgccggaact catccttcag cagaatgttc
     4141 tcgtagcgca gctcgcggtt ctcgcggcag cgccggagga ggagcagaag atgatcggca
     4201 tcgaccggct tctgcacgaa atcgtacgcg ccaagcttca tcgcctcgac cgcgttctcg
     4261 atcgtggcaa acgcggtcat cacgatcacc gggcaatcgg gatcgctctc tttgacttga
     4321 cgcagcactg caatcccgtc cgcccccggc atgcgcagat cggtgagcac ggcaaagtat
     4381 cgattgcccg ccgccagctt ccgtacggcc tccttcccat cgagcgcgac atccgcctcc
     4441 agcccctcgg cctcaacggt ctcgcgcaac atcagcgcga gcgattcctt gtcttcgacg
     4501 atgagcaggc gcttcatgag tcctaccgga tgtccaggaa gtcgggcgag gcgatcatgt
     4561 ttcgccggcg taatggctcc ggaccatttc tcttcgcctg ctcgaacgaa cgcggcatca
     4621 gctcgagtcc cggatcgagc acgaggagtc catcctgcgg cccaaccgaa gtggtccacg
     4681 gcggagcggg aatccctgcc cttcctgcca acagttccgc caagccagca gcaacgatca
     4741 tctcgcgctc gtccagtccc gcaggcttct cgtaccgctc ccactcgatc cgggcacgtt
     4801 gcgagtcagc aacccattga cgggcagcga gcagatcccc ggtcaggatc gcgtgaacca
     4861 gatcagcgag atccatgagt ctctcccaag aacatcaaac caccccacga gctcacagcc
     4921 cgaaccacgc ttttaccctt gagacgatcg tggtctcttc ctcttccgac gcctcgctgc
     4981 ggatggcgtc gaggtggccg cggcggtgga cgactttggc ggtgattgcg ctcgccagtt
     5041 cggggtggcc gtgcaggatg ggctgtaggg agtccttgcc gatttccagg gcggttacgt
     5101 cggtcagggc gacgacgtcc gcggttcggg tctcgccggt caggagggcc atctcgccga
     5161 agaccgtgcc cgggccgagt tgggcgacct cgtgccatcc ctcgggtgag tcatcgggca
     5221 ggcgcacgct ggcggtgccg gcatggacga cgaacatcga gtcgccggct gcgccgtggc
     5281 ggagtatcgt ctcccccttc gaatagaagt ggatgcgcgt ggctgcggcg atcgttgcac
     5341 gcgcctcctc ggaaagcggc gagaggacat ccacctcgct caagcgcgag cgcacatcat
     5401 cgtcgctgat ggcgtgttga acgtcgggag gcatgtattg ctggtaggcg cggatcggcg
     5461 tggcgaacgc gatcttgttt cggtgcaacg cgtaccagac ccccttgcga atgtcggcat
     5521 cgatgcggtc gcgctccgag taatcgcggg tgtggtattt gatctcgtag atgaccgcgg
     5581 agtctgcgaa cgacgcgaca cgcgcgaagc acggcagatc acgcgctacg ccgtcgacgt
     5641 gcgcggcgac ctggctgaga atcccgatca cggttgccgg cggaatgttg tagtcaacgc
     5701 tgaccggcag cacgcgacct gtgaggcggc ccagaggaaa cacctccaac cgttcgcggg
     5761 cgatgaccga gttaggaaga acgatcgcct gattgttgaa gccgcgaatc cgcgtggcgc
     5821 gccagctcac actctcgacc acgccgacgt agtcccccga gtggagcaca tcaccgacgt
     5881 tgaagcctcc ctccatgtgc atggcaatgc cgccaaacag attgccgagg gtgtcctgca
     5941 gcgccagacc gatgactgcg gcgaggactg cgtcggtcgt gagcgccgtc cggacgtcat
     6001 agtcgaag
//
LOCUS       DATLVW010000477         8043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_390936, whole genome shotgun sequence.
ACCESSION   DATLVW010000477 DATLVW010000000
VERSION     DATLVW010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390936"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VNN25_14320"
     CDS             <1..440
                     /locus_tag="VNN25_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92754.1"
                     /translation="TERNGPITERNGPITERNGPITEQNGPMTEQNGPMTDQNGPMTD
                     QNGPITERNGPMTERNGPMTERNGPMTDQNGSITEQNGPMTDQKRTDHGTKRTDDGTK
                     RTDDGRKRTDGVGVPTCRRAGSVVIYGGTPSLSLTRIASSASA"
     gene            complement(383..2065)
                     /gene="ftcD"
                     /locus_tag="VNN25_14325"
     CDS             complement(383..2065)
                     /gene="ftcD"
                     /locus_tag="VNN25_14325"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXH92755.1"
                     /translation="MKLVECVPNISEGRRADVYEAVAAAAAGVNGVTLLNVDPGADTN
                     RTVITFVGEPEAVLEGAFQLIRKGIELIDMSTHRGAHPRIGAVDVVPFIPVSGVTMDD
                     CADLARRLGERVGRELRVPVYLYEYACSAPHRRNLADVREGEYEGCARKLADPRWVPD
                     FGPAEFVPRTGAIVIGARKFLVAYNVNLNTLDKRLATRVAFDVRERGRMKRDDDGNPI
                     VDAKGEPMWEPGLLKSVKAVGWVIPEFGCAQVSINLTDLDIAPLHVVFDTCEERARER
                     GLRVTGSEIVGLVPRDVLLAAGRHYLARMGRPTGVSESALMHAAIRTLGLSELKPFDP
                     KERVIEYRLAAPARLASMTVHDFIDELSSDSPAPGGGSVSALMASMAAGLASMVAVLS
                     HTRKGFESKQMDLDRIAVRAQSLKDAFLAAVDADTAAFDQLLDAMRMPKSTPEEQGAR
                     EAAMTQATVAATEVPLGVLEACPEVIDLCMEIGRIGLQASLSDAGVGAQAARAAAAGA
                     YQNVCINLAGLNDATRRASLVHRADIAWDRVRKLHAEAEEAILVKLREGVPP"
     gene            complement(2257..3180)
                     /locus_tag="VNN25_14330"
     CDS             complement(2257..3180)
                     /locus_tag="VNN25_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXH92756.1"
                     /translation="MPPRDESLPRNTFTYFFREASRRMWVSKRTSFVAIAMIAISLLI
                     VGGFLLVADNLGRAVAQAEGKSRVNIYFDVDATPQQIATVDAWLATHPDFTSRRFVSK
                     EAALARFKQYFANLSDIVGQLDENPFPASFECDVSPIVAQSPAFARQAAELRAIVGVE
                     QVQFDWEWVETLRKLIRLVNLAGLVAGGVLAVAAAFTIANVIRLTMMLYREEIAIMRL
                     VGATERMIRGPFLIEGLLQGLIGAIVSIVLLLAAFLAGRHFLAPRNSLMWGFLFNGFL
                     PWQKVLSLLAGGMLAGYLGSWLSVRESGEEV"
     gene            complement(3187..3900)
                     /gene="ftsE"
                     /locus_tag="VNN25_14335"
     CDS             complement(3187..3900)
                     /gene="ftsE"
                     /locus_tag="VNN25_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXH92757.1"
                     /translation="MIHFYHVTKRFDQYSTALHDITFHVEKGEFVFLTGASGAGKSTM
                     LKLILRQMQPTEGQVVVNGRNLKAMKRSHLPPFRREIGMVFQDFKLIERMTVFENLSF
                     ILTVLGLPPREHKKRAYAALRAVSLHHKMNAYPLQLSGGEQQRVAIARALINDPLVIV
                     ADEPTGNLDPDLTQEIIQIFNEANLRGSTVLIATHDRELIERSGRRVITLDHGRITDE
                     KAPVPQRAPRAETLFGIGG"
     gene            4022..4402
                     /gene="crcB"
                     /locus_tag="VNN25_14340"
     CDS             4022..4402
                     /gene="crcB"
                     /locus_tag="VNN25_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXH92758.1"
                     /translation="MRFFLICLGGAIGTGARYLTSLWAAAAFGAGFPVATLIVNVLGS
                     FLIAFIMQMSSATELIRPDLRLMLTTGVMGGFTTYSTFNYETTNYFRAGAWGTGIANA
                     GVTFFGCLIAGLAGLALARLIVGR"
     gene            4415..4756
                     /locus_tag="VNN25_14345"
     CDS             4415..4756
                     /locus_tag="VNN25_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXH92759.1"
                     /translation="MRTLTGDQMMVRIFFGESDQWKHQPLEIALLERLRKEGFAGATA
                     FRGSSGFGANSIVHTTHLLDLSSDLPVVVEVVDTEEHIERLIPILDEMIDDGLVTMEK
                     VKVLRYRAGKS"
     gene            complement(4805..6685)
                     /locus_tag="VNN25_14350"
     CDS             complement(4805..6685)
                     /locus_tag="VNN25_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXH92760.1"
                     /translation="MHRQLGFIGILTVLFIAGPFSPVLQAGGVTLAKPSPRVLTAIAP
                     VVTSISPRFAPAAGGTVVTITGSNFIVGATTVKFGATAGTTVSCASTTQCTATSPAGS
                     STVSVRVTNALGTSADTSSDDFTYTAPACPDVTFGAPLGASAGTGPASIAVGDFNSDG
                     KSDLAIANAGSNNVSILLGTGSGTFGFPYTNFAAGSSPSSVAIGDFNGDGKSDLAVAN
                     SGSNNISILLGNGSGAFGPAANFAVGSTPLSVVVGDFNGDGNSDLAVVNNSSNNVSIL
                     LGTGTGTFGAPTNVTVPNDPRSLAIGDFDGDGKSDLAVANGTSNNISILLGTGLGTFG
                     PATNLTAGTLPVSVAVGDFNGDGKNDLAVANYSSNNVSILLNTGSGTFGAATNFAVAA
                     TPRLVAIADFNGDGKSDLAVAADSGHISILLGTGSGTLGAFTNYDADTSPIVAAGDFN
                     NDGRTDFVMANTVAAYAVDILLNTAACADLSITKTAVGGAFATQALTYNIAVANAGPS
                     AATAATVTDILPPGATFVSATPTQGTCSGTTTITCNLGGLASGGTANIVLRVTPTATG
                     PLSNTATVSASPADPNGANNSSTSALIVASLSNIPAVSGWAKILLALTAAILGLFMMK
                     KD"
     gene            6941..7540
                     /gene="pth"
                     /locus_tag="VNN25_14355"
     CDS             6941..7540
                     /gene="pth"
                     /locus_tag="VNN25_14355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXH92761.1"
                     /translation="MKLIVGLGNPGAEYERSRHNVGWLIVDAFAKKFRIDITKHEKSA
                     MTGEGRVAGGSVKVAKPLTFMNLSGDAVRLLVNGYLDSTDDLMIVYDDIDLPLARLRI
                     KPSGSAGTHNGMRSIVEALASERFPRLRFGVRGAEYGDGRLRDYVLDDFTAEETPLVE
                     RGIERAVDALVLFARGDLKRAMNEFNRDIVTIGEKNGGE"
     gene            7665..>8043
                     /locus_tag="VNN25_14360"
     CDS             7665..>8043
                     /locus_tag="VNN25_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HXH92762.1"
                     /translation="MNIHTLGELRTSGYRVTSIKDEMRKNLLAAIARGESLFPGIHGY
                     ERTVIPAIQNAILSRHDIILLGLRGQAKTRILRSLTNYLDERIPIIAGCEINDSPLAP
                     TCKRCRRLAAEQGDDLPIAWLTRD"
BASE COUNT         1548 a         2376 c         2655 g         1464 t
ORIGIN      
        1 tcacggaacg aaacggaccg atcacggaac gaaacggacc gatcacggaa cgaaacggac
       61 cgatcacgga acagaacgga ccgatgacgg aacagaacgg accgatgacg gatcagaacg
      121 gaccgatgac ggatcaaaac ggaccgatca cggaacgaaa cggaccgatg acggaacgaa
      181 atggaccgat gacggaacga aacggaccga tgacggatca aaacggatcg atcacggaac
      241 agaacggacc gatgacggat caaaaacgga ccgatcacgg aacgaaacgg accgatgacg
      301 gaacgaaacg gaccgatgac ggaagaaaac ggactgatgg agttggtgtt ccaacctgcc
      361 gacgagccgg cagcgttgtg atctacggcg gcacgccctc cctcagcttg acgagaatcg
      421 cctcttccgc ctcggcgtga agtttccgta cgcggtccca ggctatgtcg gcacggtgga
      481 ctaatgaggc gcgccgggta gcgtcgttca atcctgccag gttgatgcag acgttctggt
      541 acgcgccggc tgcggcggcg cgggcggcct gggcgccgac gccggcgtcc gagagcgatg
      601 cctgcagtcc gatgcggccg atttccatgc agaggtcgat gacttcggga cacgcttcga
      661 ggacgccaag ggggacttcg gtggcggcga cggtggcttg ggtcattgcc gcctcgcgcg
      721 cgccctgctc ctcgggtgtc gacttcggca tgcgcatggc gtcgaggagt tgatcgaacg
      781 cggcagtgtc ggcgtcgacg gccgcgagga acgcatcctt taatgattgg gcgcgcacag
      841 cgatgcggtc gaggtccatc tgcttcgact cgaatccttt tcgcgtgtgc gacaggactg
      901 cgaccatcga agccagcccg gctgccatcg atgccatgag cgccgagacg gagccgccgc
      961 cgggagcggg ggagtcgctc gacagctcat cgatgaaatc gtgcacggtc atcgacgcca
     1021 gacgcgccgg agcggccagc cgatactcga tcacgcgctc tttcggatcg aacggtttga
     1081 gctccgaaag tccgagggta cggatggcgg cgtgcatgag cgccgattcc gaaacaccgg
     1141 tcggacggcc catgcgcgcg aggtagtggc ggccggcggc gagcagcacg tcgcgcggca
     1201 cgaggccgac gatctccgag ccggtcacgc gcaggccgcg ctcgcgcgcg cgctcctcgc
     1261 acgtgtcgaa cacgacgtgc agcggcgcga tgtcgagatc ggtgaggttg atcgacacct
     1321 gcgcgcagcc gaattcgggg atgacccagc cgactgcctt cacggacttg agcaggcccg
     1381 gctcccacat cggctcgccc ttcgcgtcga cgatcgggtt gccgtcatcg tcgcgcttca
     1441 tgcggccgcg ctcgcgcacg tcaaaggcga cgcgcgtggc cagacgttta tccagcgtgt
     1501 tcaggttcac gttgtaggcg acgaggaact tgcgcgcgcc gatcacgatg gcgccggtgc
     1561 gaggaacgaa ttcggcggga ccgaaatcgg ggacccatcg aggatcagcg agctttcgcg
     1621 cgcagccttc gtattcgcct tcgcgcacgt ccgcgagatt gcggcggtgc ggcgcgctgc
     1681 acgcgtattc gtacaaatag acgggaacac gcagctcgcg gccgacgcgc tcgccgagac
     1741 ggcgggcgag atcggcgcag tcatccatgg tcacgccgga gacggggatg aagggaacga
     1801 cgtcgaccgc accgatacgg ggatgcgcgc cgcgatgggt gctcatgtcg atcagctcga
     1861 tacccttgcg aatgagctgg aacgcgccct cgagcacggc ctccggctcg ccgacgaatg
     1921 tgatgacggt gcggttggtg tcggcgccgg gatcgacgtt gaggagggtg acgccgttga
     1981 cgcctgctgc cgcagcggcc accgcttcat acacgtcagc tctgcgtccc tcggagatat
     2041 tcggtacaca ctcgacgagt ttcatgcggt gcgaaggcta tcgcattgga tgcgcttgcg
     2101 ccgaatgaag ggatggattg gcgttggcgt tactggtgcg gtcggcggcg aggacgccgc
     2161 cgggccagcc gccgggacgg cagcgttccg gcgcttcgcg cctcaccata cccgaccccc
     2221 gctcccaccc cctactcccc accccccaca ccccactcac acctcttccc cgctttcacg
     2281 caccgagagc cagctgccga ggtagccggc gagcatgccg ccggcgagga gggagaggac
     2341 cttttgccag gggaggaagc cgttgaagag gaagccccac atcagcgaat tgcgcggggc
     2401 gaggaagtgg cggccggcca ggaaggcggc gaggagcagg acaatcgaga cgatcgcgcc
     2461 gatgaggcct tgcaacaatc cttcgatcag gaacgggccg cgaatcatcc gttccgttgc
     2521 gccgaccagg cgcatgatgg cgatctcttc gcggtacagc atcatcgtca ggcggatgac
     2581 gttcgcgatc gtgaaggcgg cggcgactgc gagcacgcca ccggccacga ggccggccag
     2641 attgacgagc cggatcagtt tccgcagcgt ctccacccat tcccaatcga actggacttg
     2701 ctcaactccc acgatcgcgc gcaactccgc ggcctgccgc gcgaatgccg gcgactgcgc
     2761 gacgatcggg gagacatcgc actcgaacga tgccggaaac ggattctcat cgagctgtcc
     2821 gacgatgtcg gaaaggttgg cgaagtactg tttgaatcgc gccagcgcgg cttctttcga
     2881 aacgaagcgg cgtgatgtga aatcgggatg cgttgccagc caagcgtcga cggtcgcgat
     2941 ctgctgcggc gtggcgtcga catcgaagta gatattcacg cgcgacttcc cctcggcttg
     3001 cgcaacagcg cgcccgaggt tgtcggcgac gaggagaaag ccgccgacga tgagcaggga
     3061 gatggcgatc atggcgatgg ccacgaagct ggtccgcttc gacacccaca ttcgtcggct
     3121 cgcttcgcgg aagaaatacg tgaacgtgtt tcgcggaagg ctctcgtcgc gcggcggcat
     3181 cgcggttcag ccgccgatcc cgaagagcgt ctccgcgcgc ggagcgcgtt gcggaacggg
     3241 tgccttctcg tcggtgatgc ggccgtgatc gagcgtgatg acgcggcggc cgctgcgctc
     3301 gatcagctcg cggtcgtgcg tcgcaatcag caccgtcgat ccgcgcaggt tcgcttcgtt
     3361 gaagatctgg atgatctcct gcgtcaggtc aggatcgaga ttgccggtcg gctcgtcggc
     3421 aacgatgacg agcggatcgt tgatcagcgc gcgggcgatg gcgacgcgtt gctgctcgcc
     3481 gccggagagt tgcagcggat aggcgttcat tttgtgatgc aggctcacgg cgcgcagcgc
     3541 ggcgtacgcg cgcttcttgt gctcgcgcgg cggaaggccg aggacggtga ggatgaacga
     3601 gaggttctcg aacacagtca tccgttcgat cagcttgaaa tcctgaaaca ccattccgat
     3661 ctcgcggcga aagggcggga gatgcgagcg cttcatcgcc ttcaagttcc gtccgttgac
     3721 gacgacctgt ccctcggtcg gctgcatctg ccggaggatc agcttcagca tcgtcgactt
     3781 ccctgcgccg cttgcgccgg taaggaaaac gaactcccct ttctcgacgt gaaaggtgat
     3841 gtcatgaagc gccgtcgagt actgatcgaa gcgcttcgtg acgtggtaga agtggatcat
     3901 cggtcgtggg tgtgatttta cgtaggtcgg actctctagt ccgactggtc atcgagtcgg
     3961 actggagagt ccgacctacg taaaccacta gtcgggtgct agtgtaccgg tagttaacgc
     4021 catgcgcttc ttcctcatct gcctcggcgg cgcgatcgga accggggcgc gctatctcac
     4081 gtcgctctgg gctgctgccg cgttcggcgc cggctttccg gtggccacgc tgatcgtcaa
     4141 cgtcctcggg tcgtttctga tcgccttcat catgcagatg tcgtcggcta cggagttgat
     4201 ccggcccgat ctgcggctca tgctcacgac cggcgtgatg ggcggcttca cgacctactc
     4261 gaccttcaac tacgagacga cgaactactt ccgcgccggg gcgtggggaa ccggcatcgc
     4321 caatgccggc gtcacgtttt tcggttgtct cattgccggg ctggccgggc tggcgctggc
     4381 gcggctaatc gttggtcggt aaggggaggt aacgatgcga acgttgacgg gcgaccagat
     4441 gatggtgcgg attttcttcg gcgagtcgga ccagtggaag catcaaccgc tggagatcgc
     4501 gttgctcgag cgcctgcgca aagaaggctt cgccggcgcc actgccttcc gcggatcctc
     4561 cggcttcggc gcgaacagca tcgtccacac gacgcacctc ctcgacctct ccagcgatct
     4621 gcccgtcgtc gtcgaagtcg tcgacaccga ggagcacatc gagcgattga ttccaatcct
     4681 cgacgagatg atcgacgacg ggctggtaac gatggagaag gtgaaggtgc ttcggtatcg
     4741 ggcgggaaaa agctgagatc aaaggcgttg cggaacaacg actcggccgc gcgtgagcga
     4801 gcgcttagtc cttcttcatc atgaagagac cgaggatcgc tgcggtcaga gccaacagaa
     4861 ttttcgccca gccgctgacc gccgggatgt tcgataggga agccacgatg agcgccgatg
     4921 tcgacgagtt gtttgcacca ttcggatcgg ccggcgaggc ggatacggtg gcggtgttcg
     4981 acaacggccc ggtagccgtc ggcgtcacgc gcaacacgat gtttgcggtt ccgccgctgg
     5041 ccaggccacc gaggttgcat gtgatggtgg ttgtgccgct acaggttccc tgggttggcg
     5101 tggccgacac gaatgtggcg cctgggggga ggatgtcggt gacggtggcc gcggttgcgg
     5161 cgctggggcc tgcgttggcg acggcgatgt tgtaggtgag cgcttgtgtc gcgaacgcgc
     5221 ctcccaccgc tgtctttgtg atcgacaggt cggcgcaagc tgctgtattg aggagaatgt
     5281 ccacggcgta cgccgcgacg gtattcgcca tcacgaaatc ggttctgcca tcgttgttga
     5341 agtcgccggc cgccacgatc ggactcgtat cggcgtcgta gtttgtgaac gcgccaagcg
     5401 ttccagaccc ggtgccgagc aaaatagaga tatggccgga gtccgcggcg acggcgagat
     5461 cgctctttcc gtcaccgttg aagtcggcga tagccacgag gcgcggagtc gctgcaacgg
     5521 caaagttcgt cgctgctcca aaggtccccg atcctgtgtt gaggagaatc gagacgttgt
     5581 tcgaagagta gttcgcaacg gcaagatcgt tcttcccgtc gccgttgaaa tcgccgacgg
     5641 ccacagagac cgggagcgta cctgcggtga ggttcgtggc tgggccgaac gttcccaacc
     5701 cggtgcctag cagaatcgag atgttgttcg aagtaccgtt tgcgactgcg agatcgctct
     5761 tgccatcgcc gtcaaaatcg ccgatagcca acgatctcgg gtcgttcggt acggtcacgt
     5821 tcgtgggcgc gccaaaggtt ccagtccccg tgccgagaag aatcgagacg ttgttcgaag
     5881 agttgttcac gacagcgaga tcgctgttgc catcaccgtt gaagtcgccg acgacgacgg
     5941 agagcggggt cgacccgacg gcgaagtttg cggctggacc aaaggctccc gacccgttgc
     6001 caagcagaat cgagatgttg ttcgagcctg aattggcgac ggcgagatcg cttttgccat
     6061 caccgttgaa atcaccgatt gccaccgagg acggactcga tcctgctgcg aagttggtat
     6121 aggggaagcc aaaggttccc gacccggtgc cgagcaaaat cgagacgttg ttggagcctg
     6181 cgttcgcgat ggcgagatcg ctcttcccgt cgctgttgaa gtcgccgaca gcgatggaag
     6241 ccggccccgt acccgcagat gcacctaacg gtgctccaaa cgtcacatcc ggacacgctg
     6301 gtgcggtgta ggtaaagtcg tccgaggagg tgtcggcgct cgttcccaag gcgttcgtga
     6361 cgcggacgct cactgtgctg ctgccagcag ggctagtggc ggtgcactga gtcgtcgacg
     6421 cgcagctcac ggtagtgccg gcggtggcgc cgaatttcac agtggtcgcg ccgacgatga
     6481 agtttgagcc tgtgatcgtg acgacggttc cgcctgccgc cggagcgaac ctcgggctga
     6541 tcgacgtcac cacgggagcg atcgccgtga gaacgcgtgg tgatggcttc gctaacgtaa
     6601 cgccgccggc ttgcagaacc ggcgaaaacg ggcctgcgat gaacaagacg gtaaggattc
     6661 cgatgaaacc gagctgtctg tgcatggccg tgccctcctg aggccggtcg acgcgtacta
     6721 ctcggaagcg tcgccgatga ccgcctgtgg ccaggatgga ttggcgatga ccagacgggt
     6781 ctttagaagt gatgtggatg ggaaccttcg atgctgaagc gcaggaagcc ggatgactat
     6841 agcagtattt ccgtgtttac gcagagtgcg gccgagaccg gttttcgtac tatcgatcgg
     6901 aagtggtcgc ttctgacacg tctgctaccc ttcgcggcgc atgaaattga tcgtcggtct
     6961 cggcaacccc ggtgcggagt acgaacgctc gcggcacaac gtcgggtggc tgatcgtcga
     7021 tgcgttcgcg aagaagtttc ggatcgacat cacgaaacac gagaagagcg ccatgaccgg
     7081 cgaagggcgc gtagccggcg ggagcgtgaa ggtggccaag ccgctgacgt tcatgaatct
     7141 ctccggcgat gcagtgaggc ttctcgtcaa cgggtatctc gattcgaccg acgacctgat
     7201 gatcgtgtac gacgatatcg atctgccgct ggcgcgcttg cgcatcaaac cgagcgggtc
     7261 cgcgggaacg cacaacggga tgcgctcgat cgtggaggcg cttgcgagcg agcgctttcc
     7321 gcgcttacga tttggtgtgc gcggcgcgga gtatggcgat ggtcgtctcc gcgattatgt
     7381 cctcgacgat ttcactgccg aggagacgcc gctggttgag cgggggattg agcgtgccgt
     7441 cgatgcgctc gtactctttg cccgcggcga tctgaaacgt gcaatgaacg agttcaatcg
     7501 cgatatcgtc acgattggcg agaaaaacgg tggtgagtaa gcctcaagaa aaattgaacc
     7561 acagagacac agagatcaca gagttggaat tcaatagctc tgtgtcctct gtgcctctgt
     7621 ggtgaacggt ttgtcatcgt cgacacggag aaagatccca ccccatgaac atccacaccc
     7681 tcggcgagct gcgcacttcc ggataccgcg tcacatcgat caaggacgag atgcggaaga
     7741 acctgctcgc ggcgatcgcg cgcggtgaga gcctgtttcc gggcattcac ggctacgagc
     7801 gcacggtcat cccggccatc cagaacgcca tcctctcgcg ccacgacatc atccttctcg
     7861 gtcttcgcgg tcaggccaag acgcgcatcc tccgctcgct gacgaactat ctcgacgagc
     7921 gcattcccat catcgcggga tgcgaaatca acgactcgcc gcttgcgccg acttgcaaac
     7981 gctgccgaag actggccgcg gaacagggcg acgatctgcc gattgcatgg ctcacgcgcg
     8041 atg
//
LOCUS       DATLVW010000478         8485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_517253, whole genome shotgun sequence.
ACCESSION   DATLVW010000478 DATLVW010000000
VERSION     DATLVW010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517253"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..804
                     /locus_tag="VNN25_14365"
     CDS             37..804
                     /locus_tag="VNN25_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXH92763.1"
                     /translation="MNKPLIGIASDVVHREGERDRAFVYTTYIESLKRAGAVPVLIPP
                     QPENATDVLDGLDGILLAGGDDCDPAEYGEEKHPTCETMDPRRQKNDLGLARLARQRG
                     VPTLGICLGVQVMNVAAGGTLIQDIGSAVGTEIDHASEPSDRHRHDVLIDDATKLGSI
                     LGAKELNVNSSHHQAIGRVADGLRVTAHAPDGIVEGLEDPLHPFYVGVQWHPEDMPSE
                     PSATSIFGAFVEAARRHAARKEAASPELSPVGAAHSE"
     gene            850..1695
                     /locus_tag="VNN25_14370"
     CDS             850..1695
                     /locus_tag="VNN25_14370"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXH92764.1"
                     /translation="MKRTALIAAIALLLVQQAVASPWLKEIAAAQKKAKEKNQLIFVD
                     LFADWCGWCHKFEQEVIPSEAFQNATDKMVLLRLNTEDGKDGTKLSHDYGISSLPTFV
                     VLNSDLLIAGVLRGYSPAKEFALSLQDTETKYKDFMKRVSDEPSIAKDYVKRLALAKE
                     FESRAGYSQSETRLRKLIAESAVPAGVRDDAYVELAMTQMLQKKFDDARKTIAKFQTV
                     QTKGDAYERSRLLTGDIYLQSGNAAGALAEYRAFRTKFPNSQYNRNLDMMIVQLERQL
                     GAPRR"
     gene            1740..3473
                     /locus_tag="VNN25_14375"
     CDS             1740..3473
                     /locus_tag="VNN25_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXH92765.1"
                     /translation="MRRLLPFALFLLTTNSFGQLSVGGAANAIPQQDLIKISGAVNVR
                     TGDEVHGVVTASIENGWHINSNKPLDDFVIPSALVLDATTADLVSAEYPPHTVRGFTF
                     SGGQKLAVYEGTIRIPFTAKLKSGATSIKGKLHYQACNDNVCLPPRDAEVTIDTAVVA
                     ATTTAPVAPATSANFAPLSAAPKDGAPVANDQLSAAYAAHGLPLALLILFIGGLALNL
                     TPCVFPMIPITVGFFAMQSDGRRSRRFALSLAYVIGIVITYSALGVFAALSGRMFGSW
                     LQSPAVLIGFAVLMLVLASSMFGAWEFRVPQFITSRSAGKAGLAGALTMGLFVGIVAA
                     PCVGPVVVALFTLVAAIAKPVIGLAMFASLGFGLGFPYLIALNVFPKPGEWMVQVKKA
                     MGFVLIAMAFYFLRAVIGETAFRAGVAASLLIGAVFLFASRGPRGRAMRLACATLLLI
                     AGIFFAIPPRKGAEVQWQKYDASALTAAKGKPVIIDFFATWCIPCKELDEKTFSDARV
                     AHDLDRFTRIKADLTNAEDPVVKELTKRYAIVGVPTVVFIDSSGHEQPQSRLTGFESA
                     EQFLARTQQVK"
     gene            3470..4075
                     /locus_tag="VNN25_14380"
     CDS             3470..4075
                     /locus_tag="VNN25_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92766.1"
                     /translation="MTILSIEAVTRVRERIARAHDLANARFREAFASGPLAPMAHMAM
                     QAAALVECAAGLRVRGEIRYDVIGDESTPYIAGSRGAPVYDAFDVDRNAEAVFEYWLV
                     ISDIVGATSWRMTRLVATADEYDAALMRMQSPQIVRALIVTHLPSVDVRDDGTAFLEA
                     TVYTRAEEERVERRQLLLDVHNEFHYHGRELIAEGRGGVRI"
     gene            complement(4093..4839)
                     /gene="recO"
                     /locus_tag="VNN25_14385"
     CDS             complement(4093..4839)
                     /gene="recO"
                     /locus_tag="VNN25_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXH92767.1"
                     /translation="MRPQRTEAIILHTFPARERDKLVVFLTPDHGKVKGYAYGARSMR
                     TRFGASLEPLAKVRIGYVERENDEIVRIESVDLIRSLFPAQQNLAASVAASYLAETVD
                     TFAQSGDPAELIYRLLDRTTEALLAGASPLHVVAYAEVWTLRLAGILPSLRNCNVCDE
                     PLGRPLRFDAHAHGFVCPNCAPGDAQVIANDVAAALDAIMRTPVGEIAASEIAADVLF
                     EVRSLAAFFRRNFLGHELKSYEILSGVLRA"
     gene            4881..5051
                     /locus_tag="VNN25_14390"
     CDS             4881..5051
                     /locus_tag="VNN25_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH92768.1"
                     /translation="MRYRVVLIESDEGFAVSCPKLPGCWSQGATKAEAIENIRSAIEE
                     YLDVKLELQPRS"
     gene            complement(5048..5275)
                     /locus_tag="VNN25_14395"
     CDS             complement(5048..5275)
                     /locus_tag="VNN25_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH92769.1"
                     /translation="MIKVPSLNYNFVVAAFQRAGWVAVRQRGSHIRMHKQTPAGLRKI
                     TVPAHKPIKRSTLAKLLSDAELPLDEFLGLL"
     gene            complement(5272..5475)
                     /locus_tag="VNN25_14400"
     CDS             complement(5272..5475)
                     /locus_tag="VNN25_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH92770.1"
                     /translation="MKLHIVLEPSDEGGYTAYVPTLPGCISEGNSRDEALSNIREAIE
                     LYLEPVDDDFAATEGLEIAELVV"
     gene            complement(5491..5904)
                     /locus_tag="VNN25_14405"
     CDS             complement(5491..5904)
                     /locus_tag="VNN25_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92771.1"
                     /translation="MSESMTTHPSTAVAPRRGWLITATIGVALASLAMLTIALVMLDK
                     THFGWMVGTWFLEIISSGMIAGGLVLLAGAAFLPERKTWRGILLLVWALIAVTSPAFG
                     LMFLFPWGVLTLSLPLIIAILMRFFRTRREGLLAG"
     gene            complement(6175..6798)
                     /locus_tag="VNN25_14410"
     CDS             complement(6175..6798)
                     /locus_tag="VNN25_14410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXH92772.1"
                     /translation="MRRCICILTFLLAACAPLLLDPHDPRLNARAPEHFRVRFVTSQG
                     DFVVEVHRDWAPIGADRFYNLVTHRFFDGQRFFRVRQGVFAQFGIPGDPRLAQAWRLA
                     TIPDDPPRQPNTRGTVAYAFTTANTRATQIFINLADNRSYDTQGFAPFGMIISGMDVA
                     DRLYSGYGETSGGGMRAGHQDALFAGGNAWLNREFPRLDYIRSARLE"
     gene            7094..8377
                     /locus_tag="VNN25_14415"
     CDS             7094..8377
                     /locus_tag="VNN25_14415"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HXH92773.1"
                     /translation="MKLVVTILEETPDGAIDAIRALDADHDAIELRADAFRGRAVDWL
                     AVRAATSKPLIATNRGGGHVDVDAALAAGIDFVDVEWGGVIPNVQRDRIVLSHHDYEG
                     MPDSETLVQAMRVAGCAHVKLAVTPRDFADNARLLALLDGRSDLTVIGMGGRGLYARI
                     LAPFFGSAMQFVSVDEAHSAAPGQLALDRALAIYGPQRHSLGADTIFAITGNPAAHSG
                     SPAIHNAIFRERGVAAAYAIFETENFLDIAEPFARGERFAPKGLSVTAPFKEEALVFA
                     QRTGAEIAPNAREAGAVNTLVRIGGRIVADNTDVDGFSALIGQVSARSSAVVGAGGTA
                     RAALVALRRAGIAATVFNRTSGKLDAQPLDKLPSWRGDLVINTLPGNVAIALPPADTI
                     INAAYGVESRATFTGLDLLRAQAVRQNKLFLEACK"
BASE COUNT         1596 a         2619 c         2676 g         1594 t
ORIGIN      
        1 ccccaaacct cgcacgaatc ccgcataatc accaccatga acaagcctct catcggtatc
       61 gcctcggacg tcgttcaccg cgaaggggag cgcgaccggg cctttgttta cacgacctac
      121 attgaatcgt tgaagcgcgc aggcgcggtg cctgtattga tcccgccgca gccggagaat
      181 gccaccgacg ttctcgatgg actcgacggg attcttcttg ctggcggcga tgactgcgat
      241 ccggccgagt atggcgaaga gaaacatccg acttgcgaga cgatggatcc tcgccgccag
      301 aagaacgacc tcgggctggc caggcttgcg cgccagcgcg gcgttcccac gctcggcatc
      361 tgtctcggcg tgcaagtgat gaacgtggcg gccggaggga cgctgatcca ggacatcgga
      421 tcggcggtcg gtacggagat cgatcacgcc agcgagccga gcgaccgtca tcgccacgat
      481 gtgctgatcg acgacgccac gaaactcggc agcatccttg gcgcgaagga actgaacgtg
      541 aactcctcgc accaccaggc aatcggccgt gttgcggacg gccttcgcgt caccgcgcat
      601 gccccagacg ggatcgtcga agggctcgaa gatccattgc atcccttcta cgtcggcgtg
      661 caatggcacc ccgaggacat gccctcggaa ccgtcggcga cgtcgatctt cggcgcgttc
      721 gtcgaagccg cgcgaaggca tgcggcgcgg aaggaagcag catcaccgga attgtcaccg
      781 gtcggtgcgg cgcattcgga atagcgactt tcccggtgcc gtttgcgaag cccggacggg
      841 aggactcacg tgaaaaggac tgctctgatc gccgcgattg cgctgctgct cgttcagcag
      901 gccgtcgcct cgccctggct caaagaaatt gcggccgcgc agaagaaggc gaaagaaaag
      961 aaccagttga tttttgtcga cctcttcgcc gactggtgtg gctggtgcca taagttcgag
     1021 caggaagtga tcccttcgga ggcgtttcag aacgccacgg acaagatggt cctcctccgt
     1081 ctcaataccg aggacgggaa agacgggacg aaactgtcgc atgattacgg catcagctcg
     1141 ctgccgacct tcgtcgtgtt gaacagtgat cttttgatcg ccggcgtcct caggggctac
     1201 tctccggcga aggaattcgc gctgtcgctg caggacacgg agaccaaata caaagacttc
     1261 atgaaacggg tctccgacga accgtcgatc gccaaggatt acgtgaaacg gctggcgctg
     1321 gcgaaggagt tcgagtcgcg ggccggctat tcgcagagcg agacgcgcct gcgcaaactc
     1381 atcgctgaat ccgcggtgcc agcgggcgtc cgcgacgatg cctacgtcga gttggccatg
     1441 acgcagatgc tgcagaagaa gttcgatgat gcccggaaga cgattgcgaa gttccagacg
     1501 gttcagacca agggcgacgc ttacgagcgc tcgcggcttc tcaccggcga catctatctg
     1561 caatcaggaa acgcagcggg cgcgctggcc gaataccgtg ccttccggac gaagttcccg
     1621 aactcacaat acaaccgcaa tctcgacatg atgatcgtgc aactcgagcg gcagcttggc
     1681 gcgccgcggc ggtaggactt cgaaatgttg agtgttgaat gctgagtgtt gcgttgcggt
     1741 tgcgtcgcct tctcccgttc gcccttttcc tcctcacaac gaattccttc ggccagctct
     1801 ccgtcggagg cgccgcgaat gccattccgc agcaggactt gatcaagatc agcggcgcgg
     1861 taaacgtccg cactggcgat gaggttcacg gtgtcgtcac ggcttcgatc gagaacggct
     1921 ggcacatcaa ctcgaacaag ccgctcgacg acttcgtcat ccccagcgcg ctcgtgctcg
     1981 acgcaacgac tgccgatctc gtgtctgccg agtatccgcc gcacaccgtt cgcggattca
     2041 cgttcagcgg cggccagaag ctcgccgtct acgaaggaac gatccgcatc cccttcacgg
     2101 caaagttgaa aagcggcgcg acgtcgatca agggcaagct gcactatcag gcctgcaacg
     2161 acaacgtctg tctcccgccg cgagatgccg aggtgacgat cgatacggct gtcgtcgcgg
     2221 cgaccaccac cgcaccggtg gcaccagcga cttccgcgaa cttcgcgccg ctctccgccg
     2281 ctccgaaaga tggtgcaccc gtcgcgaatg atcagctttc cgcggcctac gcggcgcacg
     2341 gtcttccgct ggcgctcctc atccttttca tcggcggcct ggctctaaat ctgacgccgt
     2401 gcgtctttcc gatgatcccg atcaccgtcg gtttcttcgc gatgcagagc gacgggcggc
     2461 gttcgcgccg gttcgcactc tcactcgcgt acgtcatcgg catcgtcatc acctattcgg
     2521 ccctcggcgt cttcgctgct ctgagcggcc ggatgttcgg ctcgtggctg cagtcgcctg
     2581 cggtactgat cggcttcgcc gttctgatgc tcgtcctggc gtcgtcgatg ttcggcgcct
     2641 gggagttccg cgtgccgcag ttcatcacca gccgttcggc gggcaaggcc ggtcttgcgg
     2701 gcgcgctgac gatgggactc ttcgtcggca tcgttgccgc gccctgcgtc ggtcccgtgg
     2761 tggtcgcact cttcacgctg gtggcggcaa tcgcgaagcc ggtcatcggt ttagccatgt
     2821 tcgcgtcgct cggattcgga ctcggctttc cctatctcat cgcgctcaac gtcttcccga
     2881 aacctggcga gtggatggtg caggtcaaga aagcgatggg cttcgtcctc atcgccatgg
     2941 ccttctactt cttgcgcgcg gtgatcggcg agacggcctt ccgcgccggc gtcgctgcca
     3001 gtctgctgat cggcgcggtg ttcctcttcg caagccgagg accgcgggga agagcgatgc
     3061 ggctggcgtg tgcgacattg ttgctaatcg ccgggatctt ctttgccatc ccgccgcgca
     3121 aaggtgcgga agtgcagtgg cagaagtacg acgcctcggc actcacagca gcgaagggca
     3181 agccagtcat catcgacttc ttcgcgacgt ggtgtattcc gtgtaaggaa ctggacgaga
     3241 agacgttcag cgacgcacgc gtggcgcacg acctcgaccg cttcactcgc atcaaagcgg
     3301 atctgacgaa cgctgaggat ccggttgtga aggagctgac gaagcgctac gccatcgtcg
     3361 gcgtgccgac agtcgtcttc atcgattcgt cgggacacga gcagccgcaa tcgcgtttga
     3421 ccgggttcga gagtgcggag cagttcctcg ccagaacgca gcaggtcaag tgacgattct
     3481 ttcgatcgag gcggtcacac gcgtgcgcga gcgcattgcc cgcgcacacg atctcgccaa
     3541 cgcgcgcttc cgcgaagcat ttgcatccgg tccgctcgcc ccgatggcac acatggccat
     3601 gcaggctgcc gcgctggtcg aatgcgcggc cggtctgcgc gttcgcggag agatccgtta
     3661 cgacgtgatc ggcgacgaga gcacgccgta catcgccggc tcccgcggag cgcctgttta
     3721 cgacgctttc gatgtcgata ggaatgccga ggcggtcttc gagtactggc tcgtcatcag
     3781 cgacatcgtc ggcgcgacct catggcgtat gacgcggctg gtcgcaaccg ctgacgagta
     3841 cgacgccgcg ttgatgcgca tgcagtcgcc gcagatcgtt cgcgcactca tcgtcacgca
     3901 tctgccatca gtcgatgtgc gcgacgacgg cacagccttc ctcgaagcga cggtctacac
     3961 gcgtgccgaa gaagagcggg tcgaacgccg ccaactgcta ctcgacgtgc acaacgagtt
     4021 tcactaccac ggccgcgagc tgatcgccga gggtcgcggg ggtgtgcgga tctagcccgg
     4081 cactgacatt tcttacgccc gcaaaacccc gctcagaatc tcgtacgact tcaactcgtg
     4141 cccgagaaag ttacgccgga agaacgctgc cagcgagcgg acctcgaaca acacatccgc
     4201 ggcaatctca ctcgccgcga tctcgccaac cggagtgcgc atgattgcgt cgagcgccgc
     4261 ggcgacatcg ttggcgatca cctgcgcatc gccgggcgcg cagttcggac agacgaatcc
     4321 gtgcgcatgc gcatcgaacc gcaacggccg gccgagcggc tcatcgcaga cgttgcagtt
     4381 gcgcagcgac ggcaggattc cggccagccg cagggtccac acctcagcgt acgcgacgac
     4441 atgcagcggt gacgcccccg ccagcagcgc ctcggtcgtg cgatcgagca gccggtagat
     4501 cagctccgcc ggatcgcccg actgcgcaaa cgtgtcgacc gtctcggcca gatacgaggc
     4561 cgcaacgctg gccgcaagat tctgctgcgc cggaaagagc gaccggatca gatcgaccga
     4621 ctcgatgcgc acgatctcat cgttttcgcg ctcgacgtag ccgatgcgta ccttcgccag
     4681 cggttcgagc gacgccccga atcgcgtccg catcgaccgc gcgccgtacg catatccctt
     4741 caccttgccg tggtcgggcg tgagaaacac gaccagcttg tcgcgctcgc gagccggaaa
     4801 ggtgtgcagg atgatcgctt cggtgcgttg aggccgcatc gcagggagag tacattggat
     4861 ggaagtgtcg cggaggaagg atgcgctatc gagtggtgct tatcgagtcg gatgaagggt
     4921 tcgctgtgag ctgcccgaag cttccgggct gctggtcaca aggagcaacc aaagccgagg
     4981 cgatagagaa catccgttcg gcgattgagg agtacttgga cgtgaagctc gagttgcaac
     5041 cacgcagcta gagcaaccca agaaactcgt cgagtggaag ttcggcatcg gacaacagct
     5101 tggcaagtgt cgatcgcttg attggtttgt gtgctggcac ggtgatcttt cgcagcccag
     5161 ccggagtctg cttatgcatc cgaatgtgac tgccgcgctg tcgaaccgcc acccaaccgg
     5221 cccgttggaa ggccgcgaca acgaagttgt agttgagcga cggaaccttg atcaaacgac
     5281 gagctcggcg atctcgagtc cttcggtggc agcgaagtcg tcgtctaccg gttcaagata
     5341 caactcgata gcctcacgta tgttggacag cgcctcgtcg cgtgagttgc cttcgctgat
     5401 gcagccaggc aacgttggca catacgcggt gtatccgccc tcatcactcg gttcgagaac
     5461 gatatgcagc ttcatgtcgt gatgataacg ctacccagca aggaggccct ctcgtctcgt
     5521 ccgaaaaaac ctcatcagaa tcgcgatgat gagcggcagt gaaagcgtga gaacgcccca
     5581 cggaaacagg aacatgagac cgaaggccgg ggaagtgacg gcgatgaggg cccagacaag
     5641 aagcaaaatc ccgcgccagg tctttcgctc gggcaggaag gcggcgccgg cgaggagaac
     5701 gagtccgcct gcgatcatgc cggaagagat gatctccagg aaccaggtgc cgaccatcca
     5761 tccgaagtgt gtcttgtcga gcatcacgag ggcgatcgtt agcatcgcca gacttgcgag
     5821 agcgacgccg atcgttgccg tgatcagcca gccgcgccgc ggtgcgaccg ccgtcgaggg
     5881 atgagtcgtc atcgattcag acatgggcat gcctttcgtt ggtacttcgt caagttggta
     5941 ttggggagcg ggctttgcgt agtttagcag gtggatgatg cggcgtcgtt agcggcgcgc
     6001 gtcagaatgc cagcgtgatg gatccaaaag gagtgcggtc gtctcgaccg cgggcactcg
     6061 ggcgtcctcg cccgagtgct gtcaggccga ggtcaaagtt cgtcgacctt cttgttgcct
     6121 gaagcacggc ttcgttgttt ctgccgcgca acctccaaag cgctgctttg cggatcactc
     6181 caaccgcgcg ctccggatgt aatcgagccg cgggaactcg cgattgagcc acgcgttgcc
     6241 gccggcgaag agcgcgtcct gatggccggc gcgcattccg ccgccggatg tctcgccgta
     6301 gcccgagtag aggcggtcag cgacgtccat gccggagatg atcatgccga atggggcgaa
     6361 gccctgggta tcgtacgaac ggttgtcggc gaggttgatg aagatctgcg tcgcgcgggt
     6421 gttcgcggtc gtgaatgcat aagcgaccgt gccacgcgtg ttcggctgcc gcggcgggtc
     6481 atccgggatc gtcgccaggc gccaggcttg ggcaagccga ggatcgccgg ggattccgaa
     6541 ttgggcgaag acgccctggc gcacgcggaa gaatcgctgg ccgtcgaaga agcggtgcgt
     6601 gacgagattg tagaagcggt ccgctccgat cggcgcccaa tcgcgatgca cctcgacgac
     6661 gaaatcgccc tggctcgtga cgaagcggac ccgaaagtgt tcaggtgctc gtgcgttcaa
     6721 gcggggatcg tgcggatcga gcagcagggg agcgcaggcg gcgagcagga aggtgaggat
     6781 gcagatgcaa cggcgcatga ctgtcattct ccgatatctg tggacagtcg agagaccaga
     6841 gaccagagat cagagatcaa gagattagga ctctgcttct ctgatctctt atctctgctt
     6901 ctctgatctc tcgggatatt gacaccttcc tcctccgccg ctactctccc actccgatga
     6961 acgcgtctcg cacctcgtgg tggttccgcc gcagctagcg ggcagcgcgc atgcgccccg
     7021 cgtttgggaa ggggcgcgaa aactctgacg gtcagccaac ctcttctcga aatcgggaag
     7081 aggtttttgt ttgatgaaac tggtcgttac gattcttgaa gagacacccg acggcgcgat
     7141 tgatgcgatc cgtgcgttgg atgccgatca cgacgcgatc gagctgcgcg ctgatgcctt
     7201 ccgcgggcgc gcggttgatt ggctggccgt gcgcgcggcg acgagcaagc cgctcatcgc
     7261 cacgaatcgc gggggagggc atgtcgatgt ggacgctgcg ctcgccgccg ggatcgactt
     7321 cgtcgatgtc gagtggggcg gcgtgatccc gaacgttcag cgcgaccgga tcgttctgtc
     7381 gcatcacgat tacgagggga tgccggatag cgagacgctc gtacaagcga tgcgcgttgc
     7441 cggttgcgcg cacgtgaagc tggcggtgac gccgcgggat ttcgccgaca acgcccggct
     7501 gcttgctttg cttgatggga gatccgatct cacggtaatc ggcatgggcg gccgcggact
     7561 ttacgcgcgc atccttgcac cgttcttcgg atcggcaatg cagttcgtca gcgtcgatga
     7621 agcgcattcc gcggcgccgg ggcagttggc actcgatcgg gcgctggcga tttacggacc
     7681 gcagcgccat tctcttggtg ccgatacgat cttcgccatc accggcaatc ccgctgcgca
     7741 cagcggatcg ccggctattc acaacgccat ctttcgcgag cgcggcgtgg cagcggcgta
     7801 cgcaatcttc gaaacggaga acttcctcga catcgccgaa ccgttcgccc gcggggagcg
     7861 atttgcgccg aaagggctgt cggttaccgc gccgtttaag gaagaggcgc tggtgttcgc
     7921 gcagcgtact ggcgcagaga tcgcgccgaa cgcgcgcgaa gcgggcgcgg tcaatacgct
     7981 cgttcgcatc ggcgggcgga tcgtcgccga caacaccgac gtcgatggat tctctgcgct
     8041 catcgggcag gtcagcgcac gcagctcggc cgtggttggc gccggcggca ctgcgcgtgc
     8101 ggctctcgtt gcccttcgcc gagccggaat cgccgctact gtctttaatc ggacctccgg
     8161 caagctcgac gcccagccgc tcgacaagtt gccctcgtgg cgcggcgatc tcgtcatcaa
     8221 cacgttgccc ggaaacgttg ccatcgcgct accgcctgcc gataccatca tcaacgcggc
     8281 ctatggcgtt gaatcgcgag cgaccttcac cggactcgat cttcttcgcg cgcaggctgt
     8341 gcgccaaaac aagctctttc tcgaggcatg caaatgaacc tggatgatct tcgcaaggat
     8401 tacgacatcg ttcccgtcgc gcgcgagctg agcgccgacg ccatcacgcc ggtggcggca
     8461 ttcgcggcgc tctccggcga cggcg
//
LOCUS       DATLVW010000479         3800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_556334, whole genome shotgun sequence.
ACCESSION   DATLVW010000479 DATLVW010000000
VERSION     DATLVW010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556334"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VNN25_14420"
     CDS             <1..316
                     /locus_tag="VNN25_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92774.1"
                     /translation="GNPIALDTAGMPVSPEVTTTGFPPPLPPVPPASGDNTLGGGIFK
                     TDTTATTTSGEPRRDQVNPNRDRSAQPPPAPPTTTTSEPPPTDTNKPPPTDTNKPPAQ
                     PR"
     gene            335..715
                     /locus_tag="VNN25_14425"
     CDS             335..715
                     /locus_tag="VNN25_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease"
                     /protein_id="HXH92775.1"
                     /translation="MVLNLEVGNVARPDETRQVEFLIDSGAIYSVVPAAILDELGIKP
                     FAIEEFRRSDGRRIARRKGGALFRYQDRVGGADIIFGEPDDSNLLGALTLTALGLALD
                     PLRRELHPLPMILAGWRGESATER"
     gene            895..3282
                     /locus_tag="VNN25_14430"
     CDS             895..3282
                     /locus_tag="VNN25_14430"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH92776.1"
                     /translation="MPNLTWLYAGAVYAVAVALARRAGADLPRRIALLFFALVLVFFR
                     EPLTTDTINVPVDYYESLPPWANIIRHSHPSNPYINDVAMQLVPWAHAVREHWRAFEL
                     PLWEPTAASGYPLLANGQSQALSILRLLALPLSLGHAMTAEGAWKVLLALTFTFLFCR
                     RRGYSIVASSIGAIGYGFSGFMLVWLHFGHATVAAMLPAALYIVDLLAERASAGRFVF
                     ASCIWGVILTGGHPETAATAGLLAVLFTGWITFIERPFGTRREAWHFVRASGGSVATG
                     ALLAAPFLIPFAEVVRKSQRFAALSGSPHPAGILADWASTIATLQPHFFGFVPHEQPW
                     GPAIPEAITGFAGTFAVAGAIALFAHVAATRAWRSREAFILAALLISIGIIYGWPGVI
                     DVFNLAFRLAPPARLRCLFALLTAVSAAAAVDLIERGFRRPVLIGLFGASLLLLTMMA
                     FPFPTPAHRDVAVLALLPAVAVLAAATAATMLRWRGALLVLMLVIFAEHASVFGDWNA
                     VLPASKLYPPTPLVRALVKLKSVAPPNAPFRIVGYSADFFPNLATMYGLEDIRAHDPM
                     ESFRYVGMLALCGGYKTADYFAMWPSTTTRLLDYLNVRYYVMGPGRTMDAPRFTMVYD
                     GRDGRIFENHDVLPRFFATRRVTVEWDVKRFVAALAANDDWAHVAVLENLPITDDAMR
                     ADLTAAHAGPDASVTITSATSTGYSMHIVAPRHTLIASSIPWWPGWHVRADGKSIAPL
                     RINGAFFGFLLTPGQHDIRVWYAPASFRIGLGISLATMLAMAFAVWRSRRQVPEK"
     gene            3597..>3800
                     /locus_tag="VNN25_14435"
     CDS             3597..>3800
                     /locus_tag="VNN25_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92777.1"
                     /translation="MTNYHSDTLASQSLDVLYREHEGLLRSIAQYRYRICSSDAEALV
                     HDIFASFLERQPDALDPKAFLIGS"
BASE COUNT          613 a         1257 c         1184 g          746 t
ORIGIN      
        1 cggcaacccg attgcgctgg acacggccgg gatgcctgtc tcgcccgagg tcacgacgac
       61 cggttttccg ccgccgctgc cgccagtgcc gcctgcgtca ggagacaaca cgctgggtgg
      121 agggatcttc aagaccgaca ctacggcgac gacgacttcc ggagagccgc gtcgcgacca
      181 ggtcaatccc aatcgcgatc gttctgctca accgccgccc gcacctccga cgacgaccac
      241 gagcgaaccg ccgccgaccg ataccaataa gccaccgccg acggatacaa acaagccgcc
      301 ggcgcagccg aggtaggatc gaaggatggg gttgatggta ttgaatctcg aggttggcaa
      361 tgtcgcccga cccgacgaga cgcggcaggt cgagttcctg atcgattcag gcgcgatcta
      421 cagcgttgtt ccggcggcga ttctcgacga gctgggaatc aagccattcg cgatcgagga
      481 gttcagacgg tcggacggcc gcaggatcgc gcggcgaaaa ggcggcgcgc tcttccggta
      541 tcaggatcgt gtaggcggcg cggacataat cttcggtgaa cctgatgaca gcaatctcct
      601 cggcgcgctc actctcactg cgcttggcct cgcgctcgat cctctgcgac gagagcttca
      661 tcctctgccg atgatcctcg caggatggcg cggcgagtca gcgacggaac gctgacgagc
      721 gaatcgccgc atttctcaca ctttttcaga gactcctttg cgctcgatgg gaaagcactc
      781 caaagaaaga ggacgaggtg tagccagaag gtgcaagcgt gcgagaaatg cgcgcgaaga
      841 caagcgatac agccgccgac gcagcgaaat tgactcgcgt atcatccggc gccgatgccg
      901 aacctgacct ggctttacgc cggcgcggtc tatgccgttg ctgtggcgct cgcacgtcgt
      961 gccggcgccg atctgccgcg gcgtatcgcg ctgttgtttt tcgcgctggt tctcgtcttc
     1021 ttccgggaac cgctgacgac ggacacgatc aacgttccgg tggactacta cgagtcgctg
     1081 ccgccgtggg cgaacatcat caggcattcg catccatcga atccgtacat caacgacgtc
     1141 gcgatgcagc tggtgccgtg ggcacatgcg gtgcgcgagc actggcgcgc gttcgagctg
     1201 ccgctctggg agccgactgc cgcgtcgggg tacccgctgc ttgccaacgg tcagagccag
     1261 gcgctctcga tcctccggct gctcgcgctg ccgctctcgc tcggccacgc aatgaccgcg
     1321 gagggtgcgt ggaaagtgtt gctcgccctc acgttcacct tcctcttctg ccgcaggcgc
     1381 ggctactcga tcgtggccag cagcatcggc gcgatcggct acggattctc cggtttcatg
     1441 ctggtgtggc tccatttcgg acacgccacg gtggcagcga tgctgccggc ggcgctctac
     1501 atcgtcgacc tgctcgccga gcgtgcctcg gccggacgct tcgtcttcgc gtcatgcatc
     1561 tggggtgtga ttctcaccgg aggtcatccc gagacggctg ccaccgcggg gcttctggcg
     1621 gtgctgttta ccggctggat caccttcatc gagcgtccgt tcggcacgcg gcgcgaggcg
     1681 tggcactttg ttcgcgccag cggcggcagc gtcgcaacag gcgcgcttct ggcggctcca
     1741 tttctcattc cgttcgcgga agtggtgagg aaatcgcagc gcttcgcggc gctctccggc
     1801 tcgccgcatc ccgccggcat cctcgccgac tgggcatcga ccatcgcgac gctgcagccg
     1861 cactttttcg gcttcgtgcc gcacgagcag ccgtggggtc cggccattcc ggaggcgatc
     1921 accggattcg ccggcacgtt cgccgtggcc ggtgccattg ccctcttcgc gcatgtcgcc
     1981 gccacgcgcg cctggcgctc gcgcgaggcg ttcatcctcg ccgccttgct catttcgatc
     2041 ggcatcatct acggctggcc cggcgtgatc gacgtcttca atctcgcctt ccgtcttgct
     2101 ccgcccgcgc ggctgcgctg cctgttcgcg ttgctgacgg cggtctcagc tgccgcggcg
     2161 gtcgacctca tcgagcgcgg cttccgtcgt ccggtgctca tcggactctt cggcgcgagc
     2221 cttctgctgc tgacgatgat ggcgttcccg tttccgacgc cggcgcatcg cgacgtcgca
     2281 gtccttgcac ttctgccggc ggtggccgtg ctcgcggcag cgacggcggc aacgatgctg
     2341 cgatggcggg gcgcgctcct cgtgctgatg ctggtcatct tcgccgagca cgccagcgtc
     2401 ttcggcgact ggaatgcagt cctgccggca tcgaagctct atccgccaac gccgctcgtt
     2461 cgcgccctcg tcaaattgaa gtcggtggca ccgccgaacg ctcctttccg catcgtcggt
     2521 tactcggccg acttcttccc caatctggcc acgatgtacg ggctggaaga catccgggcg
     2581 cacgatccga tggaatcgtt ccgttacgtc ggcatgctgg cgctctgcgg cggctacaaa
     2641 accgcggact acttcgcgat gtggccgagc acgaccacgc gcctgctcga ctacctcaac
     2701 gtccgctact acgtgatggg accgggacga acgatggacg ccccgcggtt cacgatggtg
     2761 tacgacggcc gtgacgggcg gatcttcgag aaccacgacg tcctgccgcg ctttttcgcc
     2821 acgcgcaggg tcacggtcga gtgggatgtg aagcgcttcg tggcggcact cgccgcgaac
     2881 gacgactggg cgcacgtcgc cgttctcgaa aacctgccca tcaccgacga cgccatgcgc
     2941 gccgatctca ccgccgcgca cgctggaccg gacgcatccg taaccatcac ttccgcaacc
     3001 tcaaccggct attcgatgca catcgtcgcg ccgcgccaca cgctcatcgc cagcagcatc
     3061 ccctggtggc cgggctggca cgttcgtgcg gacggaaaga gcattgctcc cctgcgaatc
     3121 aatggagcct tcttcgggtt tctcctgacg ccgggacagc acgacatccg cgtctggtac
     3181 gctcccgcat cgtttcgcat cgggctcggc atttcacttg cgacaatgct ggcgatggca
     3241 ttcgccgtct ggcgctctcg ccgtcaagtg cctgaaaagt gactgattct gcgcggcgaa
     3301 ggaaaaaaga tggcccgaat ccgtcttttt ttgtgatgga ggggtaggga ctgccgcctt
     3361 tccgtcactc cttgtacagg agatatcaat cggcgatgag gattcgggcc ggcaacggtg
     3421 ccacgcaacg cctcctgacg gcagaaactg ggtcggtcgg gagtgcgagg ccgggcaggt
     3481 gggagtggcg ttcgggtacc cggcgtcaca gactcttgtt tgggacggac ggacggagtc
     3541 ggactgtttg tgcttccgga ggaacccgaa accgcgcaca ctcgcggtgg gcgatcgtga
     3601 cgaattatca ttccgacact ttggcgagcc agtcgctgga cgtgctctac cgcgagcatg
     3661 agggtttgct gcgctcgatc gcgcagtacc gttaccgcat ctgttcgtcc gacgcggagg
     3721 ctctcgttca cgatattttc gcgtcatttc tggaacgtca gcccgacgcg ctcgatccta
     3781 aagcattctt gatcggatcc
//
LOCUS       DATLVW010000480         4415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_64876, whole genome shotgun sequence.
ACCESSION   DATLVW010000480 DATLVW010000000
VERSION     DATLVW010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64876"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VNN25_14440"
     CDS             <1..227
                     /locus_tag="VNN25_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92778.1"
                     /translation="ASGHPKLYVPTTFADGSSMYHWDVSASPNLLMEPNINSDLPSNA
                     VDLTLNELIDIGWTPAAPTPAGRMAGRRGH"
     gene            387..1499
                     /gene="ald"
                     /locus_tag="VNN25_14445"
     CDS             387..1499
                     /gene="ald"
                     /locus_tag="VNN25_14445"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXH92779.1"
                     /translation="MRIGVPTEIKNNEYRVGMTPSGAQDLTSDGHKVYVQSGAGTGSG
                     FADDEYAAAGATILATADAVYEQAEMIVKVKEPIEADLARLKDGQLLFTYLHLAPVPD
                     LTAALLKKKITGIAYETITDERKRTLPLLTPMSEVAGRMSVHVGAYYLHKPNNGRGVL
                     LGGVPGTLPSDVVIIGGGVVGTNAAKMAVGLGARVTILDTNLDRLRQLDDIFRGSVQT
                     LASNKAHLTDACRHADLLIGGVLIPGASAPKLVTRAMVSEMKKGSVIVDVAIDQGGCV
                     ETAHATTHSDPVYEVDGVIHYCVANMPGAVPRTSTIALTNATLPYTRRIASLGFKKAI
                     SDAGLAEGVNVYDGKITYKAVAESQGRDYTVLDQLL"
     gene            1509..4010
                     /locus_tag="VNN25_14450"
     CDS             1509..4010
                     /locus_tag="VNN25_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HXH92780.1"
                     /translation="MKLRTILLSLATCVAFLGFASSALAQADLAVTKSCPSSAAAGSD
                     VSFDVTIFNGGDADATTVQLTDPLPPGMTFVSANQNSGPAFSCSTPSVGTNGTITCTI
                     ATLTSGSSADFTFVANIPPATPQGTTFTNIATASSPDDPNGENDSGAASVMVPIPQAD
                     IGVQKVGPSTAAPDTDVPYSISVDNSGPDPALSFSLTDTLPGTMTFVSIVQNSGPAFD
                     CSSHPSVGSGGTITCTIASLAAGATATFTLTGHIPPGTASGTSFSNTASVSSSTTDPD
                     PDNNSSLTFLTVASSDLAITKTGPPTATSGGTISWTITASNNGPDASSPAEFNDPLPA
                     GTTFASIVQNTGPTFVCTSPGAGNNGNVNCSIGTFASGANAQFTLTANIDPSFTGTLS
                     NTASIFGPNADTNPNNNSATAVTTVTASADLSVNKTGPATANAGTDITYTITLTNNGT
                     SNAASVSLIDAVPANTTFVSESQTTGSTFSCTKPSVGGTGTVNCTLASFGPGSATFSI
                     TVHISPGAADAVISNTANATTTTTDPNPGNNTSTALTNVTASADVSIVKSAPAAASNG
                     SNLTYSINVTNNGPSDAASVTMADTLPPNTTFVSESQLTGPAFICTNPAPGGTGAVSC
                     SIATLTAGTSATFSIVVQIAVAAPLGPSSNTATVTASSADPNPANNSSTAVTSIVLAT
                     ADLSITKTPAPGPYGTGQPLTYTIVVSNGGPSTATAVTVTDVLPAGTTFQSATPAGAC
                     SGTTTITCNAGTLANGASAAFTLTVTLPSSPGPITNTASVTAAATTADPNPANNSASS
                     TITVIPAANIPAISPLALLMLCVALAAAGAIAQRH"
     gene            complement(4024..>4415)
                     /locus_tag="VNN25_14455"
     CDS             complement(4024..>4415)
                     /locus_tag="VNN25_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92781.1"
                     /translation="SILDFAGSILDFAGSILDFAGSIPDFAVSILDLAVSILDFAVSI
                     LDFAGSKLDLAGSMLDIAGSMLDIGGSILDFDVSTDHIDVPVLHNDVEIAVSAIRHPT
                     NRLNPLTHGSLIPRDQNGATLADGAVF"
BASE COUNT          913 a         1551 c         1210 g          741 t
ORIGIN      
        1 atgcttccgg tcatccgaag ctctacgttc caacgacgtt cgccgacgga tcgtcgatgt
       61 accactggga cgtctcggcc tcaccgaatc tcctgatgga accgaacatc aactccgacc
      121 ttccgtccaa cgcggtcgat ctcacgctca acgaactgat cgacatcggc tggacgccag
      181 cggcgccgac gccagccggg cgaatggcgg gacgaagagg acactagaca gcgctggtag
      241 gtcgctgggt tccgaggtct cggatcgtgt gtgcgcgacc gcgagacctc ggaaccgcga
      301 aaccgctttg ttcggagcta gaatacgcgc cctgccgcgt ggagcttcct ccgcgcgcaa
      361 tcaccttcta acggagaacg aagctcatgc gcatcggtgt accgactgag atcaagaaca
      421 acgaatatcg cgtagggatg acgccctcgg gggcgcagga cctgacctcg gacggtcaca
      481 aggtgtacgt gcaatcaggc gcaggcaccg gcagtggttt tgccgacgac gaatatgcag
      541 ccgccggcgc caccatcctc gccaccgccg acgccgttta cgagcaggcc gagatgatcg
      601 tgaaagtgaa ggaaccgatc gaggccgatc ttgcccggct caaagacgga cagttgctct
      661 tcacctatct tcacctcgcg ccggtccccg atctcaccgc ggcattgttg aagaagaaaa
      721 tcaccggcat cgcgtacgag acgatcaccg acgagcgcaa acggacgctg ccgctgctga
      781 caccgatgtc cgaggtggcg ggaagaatgt cggtgcatgt gggggcgtac tacctgcaca
      841 agcccaacaa cggacgcggc gtgctcctcg gcggcgtgcc gggaacgctg cccagcgacg
      901 tggtgatcat cggcggcggt gtcgtcggca ccaacgccgc gaagatggcc gtcggactcg
      961 gcgcacgcgt cacgattctc gatacgaacc tcgatcgcct tcgccagctc gacgacatct
     1021 tccgcggttc ggtgcagaca ctggcgtcga acaaggcgca tctcaccgac gcctgccgcc
     1081 acgccgatct gctcatcggc ggcgttctca ttcctggcgc ctccgcaccg aagctcgtga
     1141 cgcgggccat ggtcagcgag atgaagaaag gctccgtcat tgtcgacgtc gccatcgacc
     1201 agggcggctg cgtcgaaacg gcgcacgcca ccacgcactc cgatccggtc tacgaagtcg
     1261 acggagtcat ccattattgt gtcgccaata tgccgggcgc cgtgccgcgc acctcgacga
     1321 ttgcattgac caacgccacg ctgccctaca cacgcaggat tgcgtcgctc ggcttcaaga
     1381 aggctataag tgatgcagga ctcgccgaag gcgtcaatgt ttatgacgga aagatcacgt
     1441 ataaagcagt cgccgagtcg caaggcagag actacacagt actcgatcag ttactgtaat
     1501 cttgtcccgt gaaacttcga acgatcctcc tttctctcgc cacctgcgta gcctttctgg
     1561 gattcgcttc atcggcactc gcgcaagccg acctggcggt cacaaagagc tgcccgtcga
     1621 gcgcggctgc cggcagcgac gtgtcgttcg atgtaacgat cttcaacggc ggcgacgccg
     1681 atgcaaccac tgtccagctc accgacccgc tgccgccggg gatgaccttc gtttccgcga
     1741 atcagaacag cggcccggcc tttagttgct cgaccccgtc ggtgggcacg aacggaacaa
     1801 tcacctgcac catcgccacg ctcacatccg gatcgagcgc ggatttcacg ttcgtcgcca
     1861 atatccctcc cgccacgccg cagggcacga ccttcacgaa catcgcgacc gcctcatcgc
     1921 ccgacgaccc caatggcgag aacgactccg gcgcggcttc cgtaatggtt cccatccctc
     1981 aggctgatat cggcgttcaa aaggtcggcc catccactgc ggcacctgat accgacgtgc
     2041 cctattcgat ctcggtcgac aactccggcc cagacccggc cctcagcttc tccctcaccg
     2101 acaccttgcc cggcacgatg acgtttgtct cgattgtcca aaacagcgga ccggccttcg
     2161 attgcagcag ccatccctcg gtcggctccg gaggaacgat cacttgcacg atcgccagcc
     2221 tcgccgccgg cgcgaccgcg acctttacac tcaccggaca cattccgccc ggaaccgcca
     2281 gcggcacgtc attctcgaac accgccagcg tatcgtcgtc aaccaccgac ccggatcccg
     2341 ataacaacag cagtctgacg ttcctcacgg tagcgagcag cgatctcgcc attacgaaga
     2401 ccggacctcc gaccgctacc tcgggaggca cgatctcctg gaccatcacc gcctcgaaca
     2461 acggccccga tgcgtcatcg cccgccgagt tcaacgatcc tctgccggcc ggtacgacct
     2521 tcgcctcgat cgtgcagaac accggcccga cgtttgtctg cacgagccca ggtgcaggca
     2581 acaacggcaa cgtgaactgc tcgattggca catttgcctc cggtgcaaac gcgcagttca
     2641 cgctcacggc aaacattgat ccgagcttca ccggaaccct gagcaacacc gcaagcatct
     2701 tcggtccgaa tgccgacaca aaccccaaca acaacagcgc caccgcggtc actaccgtca
     2761 ccgcgtctgc cgacctgtcg gtcaacaaga ccggaccggc gacggcgaac gccggaacgg
     2821 acatcaccta cacgatcacc ctgacgaaca acggcacgtc gaacgcagcc agcgtctcgc
     2881 tcatcgatgc cgtgcctgcg aatacgacct tcgtctccga gtctcagact accggctcga
     2941 cgttctcctg tacgaagcct tccgtggggg gcaccggcac tgtcaactgc accctcgcct
     3001 cgtttggccc cggctcggca acgttctcga tcaccgtcca catcagcccg ggcgccgccg
     3061 acgcggtgat ctccaatact gccaacgcca caacgacgac caccgatcca aatccgggca
     3121 acaacacgtc tacggcgcta acgaacgtca ccgccagcgc ggacgtttcg atcgtcaagt
     3181 ccgcgcccgc ggctgcgtcc aacggatcga acctcaccta cagcatcaat gtcacgaaca
     3241 acggcccgtc cgacgcggca agcgtcacga tggctgacac gctgccgccg aacacgacgt
     3301 tcgtctccga gtcgcagttg accgggccgg ccttcatctg cacgaacccc gctccgggcg
     3361 ggaccggcgc cgtctcctgc tccatcgcga cgctcacggc cggcacctcc gccacgttct
     3421 ccatcgtcgt gcagatcgcc gtggcagcac cgctcggacc gagcagcaac acagcgacgg
     3481 tgaccgcatc atccgccgat ccaaatccgg ccaacaacag ttccacggcc gtcacctcga
     3541 tcgtgctggc aacagcggat ctgtcgatca ccaagactcc cgctcccggc ccttacggca
     3601 ctgggcagcc gctgacgtac acgatcgtcg tctcaaacgg cggtcccagc accgccaccg
     3661 ccgtcaccgt caccgacgtc cttccagcgg gtacgacctt ccagtcggcc acgccggcag
     3721 gcgcgtgcag cggtacaacg accatcactt gcaatgccgg tacgctcgcc aacggcgcct
     3781 ctgctgcctt cacgctgacg gtgacactgc cttcgtcgcc gggcccgatt acgaacaccg
     3841 cctccgtgac cgctgcggcc acaactgctg atccgaatcc ggcgaacaac agcgcttcct
     3901 cgaccatcac cgtgatcccg gcagcaaaca ttccggcgat ctcgccgctc gcgcttctca
     3961 tgctctgcgt cgccctcgcc gccgcaggcg cgatcgcgca acgacactaa cgtttcaacg
     4021 cgctcaaaac acggcgccat ccgcaagggt ggcgccgttc tggtctcgag ggatcagaga
     4081 tccgtgcgtt agtggattca gtcgattcgt cgggtgtcgt atcgccgaga cggcgatctc
     4141 cacatcgttg tgaagaaccg ggacatcaat gtggtcagtc gagacatcga agtcaagaat
     4201 cgagcctccg atgtcgagca tcgagcctgc gatgtcgagc atcgagcctg cgaggtcgag
     4261 cttcgagcct gcgaagtcaa gaatcgagac tgcgaagtcg agaatcgaga ctgcgaggtc
     4321 gagaatcgag actgcgaagt cgggaatcga gcctgcgaag tcgagaatcg agcctgcgaa
     4381 gtcgagaatc gagcctgcga agtcgagaat cgagc
//
LOCUS       DATLVW010000481         4938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_391131, whole genome shotgun sequence.
ACCESSION   DATLVW010000481 DATLVW010000000
VERSION     DATLVW010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391131"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /gene="lpdA"
                     /locus_tag="VNN25_14460"
     CDS             <1..1332
                     /gene="lpdA"
                     /locus_tag="VNN25_14460"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXH92782.1"
                     /translation="RAGQLGMKVAVVEKDPFLGGTCLHRGCIPTKSLLENADIWTKIQ
                     KSKEFGITVGDVTIDWSVVQTRKQGVVDGQAKGIEFLFRKNKVERINGFAKLAGKGIV
                     EVTGEGGTQRLNTKNVILAMGSVPKDLPHIKTDGKRIINSDHILRIGSIPKSMLVIGA
                     GAVGCEFASIFARFGTKVTIVEVMPLLLPIEDEEISKEFTRVFKKKGIEVLTDAKVES
                     AEVNDAGVKVVVSSKGKQQTFDVEVVLSATGRRAVTENCGLEGTKVVIERGFVQVDPY
                     MRTGEANIYAIGDIVPTPALAHCASAEGILTVEKIKGLEVRPINYDHVPNATYTDPEV
                     ASVGLTEKKAKERGYDVKIGKFPFTANSKAKILGDANGLVKFVSDKKYDELLGVHIVG
                     PKATELIAEACAALELEATSESIAKTIHAHPTLSEAMMEAAEDVGGHSIHQ"
     gene            1478..2497
                     /locus_tag="VNN25_14465"
     CDS             1478..2497
                     /locus_tag="VNN25_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXH92783.1"
                     /translation="MTDTPLETHGLTRENLLEIDYFLRLTRTLEERLVALFRQAKVIG
                     GLYRSLGQEGESVAAAYALDYKQGDVVQPLIRNMGSILVAGAKPSDIIKQYMAKGDSP
                     TRGRELNIHFGHPDRDGFIGQISMLGDMIPVMAGITLAGRMQKRNLVGMAFIGDGASS
                     TGAFYEGMNFATVQRLPLVVILENNGYAYSTPTAKQSAVKTLADKAHAFGCASEVVDG
                     NDVLATYAAAKRAVDRARAGEGVTLIEVKTFRIKGHAEHDNQSYVPIQLIEEWSAKDP
                     VVQFEKALIDAGLAATSDFDAIQARVRNEVDTATDEAERSPMPRPEEAALGLFAGDGY
                     WERQQ"
     gene            2625..3599
                     /locus_tag="VNN25_14470"
     CDS             2625..3599
                     /locus_tag="VNN25_14470"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXH92784.1"
                     /translation="MALTTYLDAIREAMIEEMRRDPRVFLIGEDVGSYGGAFKTSAGL
                     IDEFGPGRVIDTPISEVAIVGAASGAALMGMRPIAEMQFIDFITCAYDMLVNFTATSR
                     YRVGSSCPIVVRGPAGGGTHGGPFHSLNPEAMFMNRPGIKIVAPGTAYDAKGLLKAAI
                     RDDDPVLFIEHKFLYRRIKEDLPTEDYVVPIGKANIRRTGKDITIITWGAMIYVADDA
                     AADLATEGVSAEVIDLRSIIPFDEEAILNSVAKTNRVIILHEAPLTGGAGAEFAARIA
                     EKAFDYLDAPVKRVAALDVPTPYSPPLEAAALPDKARVLAAARELLRY"
     gene            3636..>4938
                     /locus_tag="VNN25_14475"
     CDS             3636..>4938
                     /locus_tag="VNN25_14475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXH92785.1"
                     /translation="MPIDVVMPQMGESIAEGTVVKWLKKPGDKVERDEPLLEISTDKV
                     DAEIPAPASGTLTEIIAQEGQTVGVNTVVARIAGEGEAAGASAPAQPAAAPAQPAPTA
                     TAPAPASAPPATAPPASAPPAQAQAPAPSPKPAAPAPQAPPAPKPTEQPSNVATPQPR
                     EENITSLDERRRTKSSPLVRKIAEENNVDISRLQGSGVSGRVTKNDILDYLQQPGAAA
                     EAAPALAEGGAGIRPAPAESTVAAATRPAMTFVPGEGDRIEPLSMMRKKIAQHMVLSK
                     QTSAHVTTVFEVDFTNIDKLRRRHKDAYAERGAKLTFLPFVIQAVVQGLREFPIINAS
                     MDESSVTYHRDLNIGIAVALEWGLIVPVVKNADEKNILGLSKSINDLGERARTKKLSP
                     DDVQGGTFTITNPGIYGGLFGTPIINQPQVAILGVGGVKKRP"
BASE COUNT         1080 a         1659 c         1414 g          785 t
ORIGIN      
        1 cgcgccggcc agctcggcat gaaggtcgcc gtcgtcgaaa aagatccatt cctcggtggg
       61 acgtgcctgc accgcggctg catccccacc aaatcgctcc tcgaaaatgc agacatctgg
      121 accaagatcc agaagtcgaa ggagttcggc atcaccgtcg gcgacgtgac gatcgactgg
      181 agcgtcgtcc agacccgcaa gcagggggtc gtcgacggcc aggccaaagg catcgagttc
      241 ctcttcagga agaacaaagt cgagcggatc aacggcttcg ccaagctggc cggcaaaggc
      301 atcgtcgaag taaccggcga aggcggcacg cagcgcctca acacgaagaa cgtcatcctg
      361 gcgatgggct ccgttcccaa agacctgccg cacattaaga ccgacggcaa gcggatcatc
      421 aactccgatc acatcctccg catcggctcc atcccgaagt cgatgcttgt catcggcgcg
      481 ggcgccgtcg gctgcgagtt cgcatcgatc ttcgcccgct tcggcacgaa ggtcacgatc
      541 gtcgaagtga tgccgctgct gctgccgatc gaagacgaag agatctcgaa agagttcacg
      601 cgcgtcttca agaagaaagg catcgaggtt ctgaccgacg cgaaggtcga gtcggccgaa
      661 gtcaacgacg ccggcgtgaa agtggtcgtc agctcgaaag gaaaacagca gaccttcgat
      721 gtcgaagtcg tcctctccgc caccggccgc cgcgccgtga cggagaactg cgggctcgaa
      781 ggaaccaaag tcgtcatcga gcgcggcttc gtgcaagtcg atccgtacat gcgcacgggc
      841 gaagcaaaca tctacgccat cggcgacatc gttcccaccc ccgccctcgc ccactgcgcc
      901 tcggcggaag gaatcctcac cgtcgagaag atcaaaggac tcgaagtccg tccgatcaac
      961 tacgaccacg tgcccaacgc gacgtacacc gatccggaag tggcatcggt cggcctcacc
     1021 gagaaaaaag cgaaagagcg cggctacgac gtcaagatcg gcaagttccc gttcaccgca
     1081 aactcgaaag cgaagatcct cggcgatgcg aacggcctcg tgaagttcgt ctccgacaag
     1141 aaatacgacg agctcctcgg cgtccacatc gtcggcccga aagcgaccga gctgatcgcc
     1201 gaagcgtgcg ccgccctcga gctcgaagcg acgagcgaat cgatcgccaa aaccatccac
     1261 gcgcatccaa cgctgtccga ggcgatgatg gaagcggcgg aagacgtggg tgggcattcg
     1321 atacatcagt agggtgcaga gacaggagat cagagactag agatcaggtt aagtactgat
     1381 ctttctccga tctctgatct ctaatctcac tgtttctgag tcccttgtct ctgatctctt
     1441 gtctctgatt tccaagcgcc gatccaccaa ttcacccatg accgacaccc cactcgaaac
     1501 ccacggcctc actcgcgaga atcttctcga gatcgattac ttcctccgcc tgacgcggac
     1561 gctcgaggag cggctcgtcg cgctgtttcg gcaggccaaa gtaatcggcg ggttgtatcg
     1621 ctcgcttggc caggaaggcg aatcggtcgc cgcggcgtac gcactcgact acaagcaggg
     1681 cgacgtcgtg cagccgctga tccgcaacat gggatcgatc ctcgtcgccg gcgcaaagcc
     1741 ctccgacatc atcaaacagt acatggccaa aggcgactcg ccgacgcgcg ggcgcgagct
     1801 gaacatccat ttcggacatc cggaccgcga cgggttcatc ggccagatct cgatgctcgg
     1861 cgacatgatt ccggtgatgg ccggcatcac cctcgccggc cggatgcaga aacgcaacct
     1921 cgtcggcatg gcctttatcg gcgacggcgc gtcgtcgacc ggcgcgtttt acgagggaat
     1981 gaacttcgcc accgtacagc gcctgccgct ggtcgtgatc ctggaaaaca acggctacgc
     2041 ctactcgacg ccgacggcga aacaaagcgc agtgaagacg ctcgccgaca aagcgcacgc
     2101 cttcggctgc gccagtgaag tcgttgacgg caatgacgtc ctcgccacct acgcggccgc
     2161 gaagcgcgcc gtcgatcgcg cccgcgcagg cgaaggcgta accctcatcg aggtaaagac
     2221 tttccgtatc aaaggccacg ccgagcacga caaccaatct tatgtcccga tccagttgat
     2281 cgaggagtgg tcggcgaaag atccggtcgt gcaattcgag aaagcgctca tcgatgccgg
     2341 ccttgccgcg acgtcggact tcgacgcgat ccaggcgcgc gtccgcaacg aagtcgatac
     2401 cgctaccgac gaagccgaac gctcgccgat gccgcgccca gaagaagcgg cgctcggttt
     2461 gttcgccggg gatgggtact gggagcggca gcaatgatgc gttggaacgc tggcgtcctc
     2521 gccggctggc ccggcggcgt cctcgccgcc gacccgccag acaccaaaca cacgcagaga
     2581 acacggctgg tcgccaacgc tccatcgttc atgcggagtc gccgatggca ctaaccacct
     2641 acctcgacgc cattcgcgag gcgatgatcg aagagatgcg ccgcgatccg cgcgtgtttc
     2701 tcattggaga agacgtcggc tcgtacggcg gcgcgttcaa aaccagcgcg gggctgatcg
     2761 atgaattcgg tcccggacgc gtcatcgaca cgccgatctc cgaggtggcg atcgtcggcg
     2821 cggcatcagg ggcggcgctc atgggcatgc gcccgatcgc ggagatgcag ttcatcgact
     2881 tcatcacctg cgcgtacgac atgctcgtca acttcacagc gacctcgcgc tatcgcgtcg
     2941 gcagctcatg cccgatcgtt gtgcgcggtc ctgccggcgg cggaacgcac ggcggaccgt
     3001 ttcactcgct gaaccccgag gcgatgttca tgaaccggcc gggaatcaag atcgttgcgc
     3061 cgggaactgc gtacgacgcc aagggtctgc tcaaggccgc cattcgggac gacgatccgg
     3121 tgctcttcat cgagcacaag ttcctctatc gccggatcaa agaggatctg ccaactgagg
     3181 actatgtcgt gccgatcggc aaggccaaca tccggaggac cggcaaggac atcaccatca
     3241 tcacctgggg cgcgatgatc tacgtcgccg acgatgctgc cgccgatctg gcaaccgaag
     3301 gcgtctccgc cgaagtcatc gaccttcgct cgatcatccc gttcgatgaa gaagcgatcc
     3361 tgaattcggt ggcgaaaaca aatcgcgtca tcatcctgca cgaagcaccg ctgaccggtg
     3421 gcgcgggcgc cgagtttgcc gcgcgcatcg ccgagaaagc gttcgattac ctcgatgcgc
     3481 cggtcaaacg ggtggccgcg ctcgacgtac ctacgcccta ttcaccgcct ctcgaagccg
     3541 ctgctcttcc cgacaaggcc cgcgttctcg ccgcggcgcg ggagttgctg cggtactgac
     3601 acttgcatta aaacgattcc cgccaggaga tacccatgcc aatcgatgtc gtcatgccgc
     3661 agatgggcga gtcaatcgcc gaaggaactg tcgtcaagtg gctcaagaag ccgggcgaca
     3721 aagtcgagcg cgatgagccg ctcctcgaga tctcgaccga caaggtcgac gccgagattc
     3781 cagctcctgc gtcaggaact ttgaccgaga tcatcgccca ggaaggccag accgtcggcg
     3841 ttaacaccgt cgtcgcgcgc atcgccggcg aaggtgaagc agccggcgcg agcgcacctg
     3901 cacaaccagc cgcggcaccc gcacaacctg ctccaactgc aactgcgccg gcaccagctt
     3961 ctgctccgcc tgcaaccgcg ccaccagcgt cggctccgcc agcacaagcg caagcaccag
     4021 ccccatcccc gaaaccggcc gctcctgcgc cacaagcgcc acccgcacca aaaccaactg
     4081 agcaaccgag caacgtagca accccgcaac ccagagaaga aaacatcacc tcactcgacg
     4141 agcgccgccg cacgaaatcc tccccgctcg tccgcaagat cgccgaggag aacaacgtcg
     4201 acatctcgcg actgcagggt tccggcgtct ccggccgcgt gacgaagaac gacatcctcg
     4261 actacctcca gcaaccaggc gcagcggcag aggcagcacc cgcacttgcc gagggcggcg
     4321 cgggaattcg accagcaccc gcagagagca cagtcgccgc cgcaacgcgc cccgccatga
     4381 ccttcgtacc cggcgaaggc gatcgcatcg agcccctctc gatgatgcgc aagaagatcg
     4441 cccagcacat ggtcctctcg aagcagacct cggcgcacgt gacgaccgtc ttcgaagttg
     4501 acttcaccaa catcgacaag ctccgccgcc gccacaagga cgcctacgcc gagcgcggcg
     4561 cgaaactcac gttcctgccg ttcgtgatcc aggccgtcgt tcaaggtctc cgcgagttcc
     4621 ccatcatcaa tgcatcgatg gatgagtcgt cggtcacata ccaccgcgat ctcaacatcg
     4681 gaatcgccgt cgctctcgaa tggggactga tcgtccccgt cgtcaagaac gccgacgaga
     4741 aaaacatcct cggcctttcc aagtcaatca acgacctcgg cgagcgtgcc cgcacgaaga
     4801 agctctcgcc cgacgatgtt cagggcggca cgttcacgat cacgaatccc ggcatttacg
     4861 gcggcctctt cggaacgccc atcatcaacc agccgcaagt cgccatcctc ggcgtcggcg
     4921 gcgtgaagaa gcgtccgg
//
LOCUS       DATLVW010000482         5321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_310979, whole genome shotgun sequence.
ACCESSION   DATLVW010000482 DATLVW010000000
VERSION     DATLVW010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310979"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VNN25_14480"
     CDS             <1..260
                     /locus_tag="VNN25_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92786.1"
                     /translation="LPFYDAAGRRPSSNVHKAWISGDSLSRNDLIGKNVLCLRVELRR
                     VVIDDLEVSGRFSDQDELARRFHFSSVDPACIFDALDKLFE"
     gene            complement(497..1084)
                     /locus_tag="VNN25_14485"
     CDS             complement(497..1084)
                     /locus_tag="VNN25_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92787.1"
                     /translation="MKVAFGMKAHSGWAALVVLGISDGEFAVVDRRRVELVEEEWAKQ
                     PYHAAEEMKGAEAPDLVGRGIEAARRIAVREMRAAVKREKERKNEVVACAVLTGNPMP
                     DWSVEEILSVHFRMHKAEGVLFRDVLARAAESCALKLVAIPEKQLMKHAEKALGLAAS
                     TLVSNVAALGKIVGPPWGKDQKDAALAAMVALREI"
     gene            complement(1248..2558)
                     /gene="nuoF"
                     /locus_tag="VNN25_14490"
     CDS             complement(1248..2558)
                     /gene="nuoF"
                     /locus_tag="VNN25_14490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXH92788.1"
                     /translation="MDQIKVLLANIDKPNSTSIDVYLDGGGYKAVEKALKMTRDEVTQ
                     LVKDSEMRGRGGAGFPTGMKWSFVPKNAPKPTWLLCNADESEPGTFKDRVIMEKDPHL
                     LIEGMIISAYAIGSNPMAYIYIRGEFYYGATVLDKALDEARAKGYLGKNILGSGFDFD
                     ITVARGAGAYICGEETGLIESVEGHRGQPRVKPPFPAVVGVFGGPTVVNNVETLACVP
                     LIINNGAEWFKSFGTPKNTGPKLYCVSGSVRKPGVYEFPMGINLKELIYDHCGGILGD
                     RKLKAVIPGGASAPMLKPDEIDLPLNFDAVQKAGSMLGSAGIIVLDDSVCIVDAVWRL
                     AKFFAHESCGQCTPCREGTNWLESVLDRIERGEGRPQDADLLLSMSDNIGGKSLCALG
                     DAAIGPVISSVKKFRDEFMYHIEHKSCLPETVRYRRLDRAVAAH"
     gene            complement(2561..3091)
                     /locus_tag="VNN25_14495"
     CDS             complement(2561..3091)
                     /locus_tag="VNN25_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXH92789.1"
                     /translation="MADLHPDNPYAPDRFPDRNKPVEFPPEAKKKFDYILTRYPTKEA
                     ALLPTLHLVQEVWGWISPEAVLYVGELLDLSPATVFGVVSFYNMYNQKPVGKYHLQVC
                     TNLSCMVRRAYDIYDHLCDKLDVQPGATTKDGQYTVVEVECLGSCGTAPVVQVNNEYH
                     ENMDVTKMDALLAELK"
     gene            complement(3094..3462)
                     /gene="ndhC"
                     /locus_tag="VNN25_14500"
     CDS             complement(3094..3462)
                     /gene="ndhC"
                     /locus_tag="VNN25_14500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXH92790.1"
                     /translation="MTHTLLPVLIMMLIAVVVGVVMLALAWFVGGHARTASKQEPYES
                     GVPSLDENRKRVNVRFYQIAMLFILFDIEAAFLYPWAVIYREASTGAQGMFLFGEMLV
                     FIVLLAVGYVYVWKKKAFDF"
     gene            complement(3553..3861)
                     /locus_tag="VNN25_14505"
     CDS             complement(3553..3861)
                     /locus_tag="VNN25_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016924212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXH92791.1"
                     /translation="MIIHLTSATYLRDGQFELAFNDGRNGVADLSGMLEGPIFEPLRD
                     PEFLARGTLDPETRTLSWPNGADVAPEFLYFLAFRDDHDLSGLFQEWGYLREQTVASR
                     "
     gene            complement(3874..4101)
                     /locus_tag="VNN25_14510"
     CDS             complement(3874..4101)
                     /locus_tag="VNN25_14510"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXH92792.1"
                     /translation="MISMFWNDHAPPHFHARYGEYEITVEIESGVVEGKFPPRSLNHV
                     IEWHRANRGELLLAWEQCRRGEAPRPIAPLE"
     gene            complement(4162..4512)
                     /locus_tag="VNN25_14515"
     CDS             complement(4162..4512)
                     /locus_tag="VNN25_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXH92793.1"
                     /translation="MNIIKNRVDDVTVLDIQGVIKLGESAREFSSYLEKVLNDETGPV
                     MINFEAINYMDSTGLGELIGYLQKFEDRQRKMALVKPSHRILALLKITKLDTVFKIFD
                     SRETAMEYLTDTAA"
     gene            complement(4587..4940)
                     /locus_tag="VNN25_14520"
     CDS             complement(4587..4940)
                     /locus_tag="VNN25_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXH92794.1"
                     /translation="MHLILRWALNTLALFVVVTVVPGFHYRSIVSLAIAALILGLLNA
                     IVRPILVFLTLPLTIVTLGLFLIVLNAVMLELTAWLSPGFRIDDFVAALIGAIVLAII
                     SWVTNQIGRKPEKER"
     gene            complement(5058..>5321)
                     /locus_tag="VNN25_14525"
     CDS             complement(5058..>5321)
                     /locus_tag="VNN25_14525"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXH92795.1"
                     /translation="DEPDVVLVRVEFVGEGRRLRYDIIDRYDEATGLSAMMRTTAFPA
                     SIVALMMARGQTTQKGALPQERCIPPELFMQELARRKIVVHMS"
BASE COUNT         1030 a         1552 c         1632 g         1107 t
ORIGIN      
        1 cgctgccttt ctacgacgcc gcaggtcgac gtccatcttc taatgttcac aaggcgtgga
       61 tatcaggtga cagcttgtca aggaatgacc tgataggaaa gaacgtcctc tgcctccgcg
      121 ttgagctccg ccgcgtggtt attgatgatc tcgaggtcag tggaagattt tcggatcaag
      181 acgaactcgc gcgacgcttt catttctcct cggtggatcc tgcctgcatc tttgatgctc
      241 tcgacaagct cttcgaatag atccttttca tttgaggtcc tgtgccacca tcttgcgtaa
      301 ctcactttcc ctgccgcggt aggtcaacgc gttcgttttc cgagggtcgc ctcggcatac
      361 gcacgcttgc ccgagcgaag aacgtgtcgt cgagcgacgg aacatcggac gtgtcgatct
      421 cggcatcgga gagtgctgcg accttattcc agtcggtttt ggagctagct gtcgacgttc
      481 tatcgctcat cgcaattcag atctcccgca gcgcaaccat cgccgccagc gccgcatcct
      541 tctgatcctt cccccacggc ggcccgacga tcttcccgag cgccgcaacg ttgctgacga
      601 gagtactcgc cgcgaggccg agcgccttct cggcgtgctt catcagttgc ttctcaggga
      661 tcgcgaccag cttcagcgcg caggattccg cggcgcgagc cagcacgtca cggaaaagca
      721 ctccctcggc cttgtgcatg cggaaatgca ccgacaggat ctcctcgaca ctccaatcgg
      781 gcatcgggtt cccggtcagc acggcgcaag cgactacctc gttcttccgt tccttctctc
      841 gcttcaccgc ggccctcatc tcccggaccg caatccgccg cgcagcctcg atccctcggc
      901 cgaccagatc gggcgcctcg gcgcccttca tctcttcagc agcgtgatac ggttgttttg
      961 cccactcctc ttcaacgagc tcgacgcggc gccggtcgac aaccgcgaat tcgccgtcgc
     1021 tgatgccgag gacgacgagc gcggcccatc cggagtgtgc cttcatcccg aacgcgacct
     1081 tcatttctca aacctcggct gagagtgcgc cacgccacaa attctagacc gtcggactgg
     1141 cgaagtctct caccacagag acacagagat cacagagaac ggttcaactc cgtgtcctca
     1201 gtgtctctgt ggttcatgtt tgccctggcc atcgggcgag atgcactcta atgcgccgcg
     1261 accgcgcgat ccagcctgcg ataccgcacc gtttcgggga ggcagctctt gtgctcgatg
     1321 tggtacataa actcgtcacg gaacttcttg acggacgaga tgaccgggcc gatggcggcg
     1381 tcgccgaggg cgcagaggga tttgccgccg atattgtcgg acatcgacag gaggaggtcg
     1441 gcgtcctggg ggcggccttc gccgcgttcg atgcggtcga ggacagattc gagccagttg
     1501 gtgccttcgc ggcagggggt gcattgaccg cagctttcgt gggcgaagaa tttggcgagg
     1561 cgccagacgg cgtccacgat gcagacggag tcgtcgagga cgatgatgcc ggcggagccg
     1621 agcatggagc cggctttctg gaccgcgtcg aagttgagcg ggagatcgat ctcgtcgggc
     1681 ttgagcatgg gtgccgaggc gccgccgggg atgacggctt tcagcttgcg gtcgccgagg
     1741 attccgccac agtggtcgta gatgagctct ttgaggttga tgcccatcgg gaactcatag
     1801 acgcccggtt ttctcacaga gcccgacacg caatagagct tcgggccggt gttcttgggc
     1861 gtgccgaatg atttgaacca ctccgcgccg ttgttgatga tgagaggcac gcaggccagc
     1921 gtttcgacgt tattgacgac ggtcggtccg ccgaagacgc cgacgacggc aggaaacggt
     1981 ggcttgacgc gcggctggcc gcggtggcct tcgaccgact caatgaggcc ggtctcttcg
     2041 ccgcagatgt aggcgccggc gcctcttgcg acggtgatgt cgaagtcgaa gccgctgccg
     2101 agaatgttct tgccaaggta gcctttggcg cgggcttcgt cgagagcttt gtcgaggacc
     2161 gtggctccgt aatagaactc gccgcggatg tagatgtacg ccatcggatt ggagccaatg
     2221 gcgtacgcgg aaatgatcat cccttcgatg agaaggtgcg gatccttttc catgatgacg
     2281 cggtctttga aggtgccggg ctcggattcg tcggcattgc agagcagcca agtgggcttg
     2341 ggcgcgttct tgggaacgaa actccacttc atgccggtgg ggaagcccgc gcctcccctg
     2401 ccgcgcatct cggagtcttt gacgagttgc gtcacctcat cgcgcgtcat cttgagcgct
     2461 ttttcgacgg ccttgtagcc gccgccatcg agatagacgt cgatcgacgt cgaattcggc
     2521 ttgtcgatgt tggcgagtag gactttgatc tgatccatta ctacttcaac tccgccagca
     2581 gcgcgtccat cttcgtgacg tccatgttct cgtggtactc gttattgact tgtacgaccg
     2641 gcgcggtgcc acaggagccg aggcattcca cttcgacgac ggtgtactgg ccgtctttgg
     2701 tcgtagcgcc cggttgaaca tcgagcttgt cgcaaagatg gtcgtaaatg tcgtaggcgc
     2761 ggcggaccat gcagctcaga ttcgtgcaga cctgcaggtg gtatttgccc acaggcttct
     2821 ggttgtacat gttgtagaac gagacgacgc cgaacaccgt cgcaggcgag agatccagca
     2881 actctcccac gtacaacacg gcctcggggg agatccagcc ccacacttcc tggacgagat
     2941 gcagtgtcgg caacagcgcc gcttctttcg tcggatatct cgtcaggatg taatcgaatt
     3001 tcttcttcgc ctcgggtgga aactcgacgg gcttgttccg atcgggaaag cgatcgggag
     3061 cgtagggatt atcgggatgg agatcagcca tggctaaaaa tcgaatgctt tctttttcca
     3121 gacgtaaacg tagccgacag cgaggaggac gatgaaaacg agcatctcgc cgaagaggaa
     3181 catgccctgt gcgccggtgg atgcctcgcg gtagatgacg gcccacgggt agaggaacgc
     3241 cgcctcgatg tcgaagagga tgaacaacat ggcgatctgg tagaagcgaa cgttgactcg
     3301 cttccggttc tcatcgagcg acggcacccc gctctcgtat ggctcctgct tggaagcggt
     3361 acgggcgtgc ccgccgacga accaggccag cgcgagcatc acgacgccga cgacgacggc
     3421 gatgagcatc atgatcagga cgggaaggag tgtgtgggtc atgaacggcg gtgaatgcta
     3481 gtcgcaaagg tggtgccggg caagggcggg gggatcgggg agattggcga ggctcgcgat
     3541 gcgctcctta ctctatcgtg aagccacggt ttgttctcga agatagcccc actcctgaaa
     3601 caagccggac agatcgtgat cgtcgcggaa tgcgaggaag tagaggaatt cgggagcgac
     3661 atccgcaccg ttcggccaac taagtgtgcg agtctcgggg tccaaggtgc cacgcgcgag
     3721 gaactcggga tcgcgtagcg gttcgaatat cggcccttca agcatcccac tcaagtcggc
     3781 gacaccattg cggccatcgt tgaatgccag ctcgaactga ccatcacgaa gatacgtagc
     3841 gctggttaga tggatgatca ttgggtcctc gatttactcc aacggcgcga taggccgagg
     3901 tgcttcccct cgccggcact gttcccaggc aagcagtaat tccccgcgat tcgcccgatg
     3961 ccactcgatg acgtggttca gggagcgagg gggaaacttt ccttcgacaa cgcctgattc
     4021 aatctcaacg gtgatctcgt actctccgta gcgcgcgtgg aagtgcggcg gtgcgtgatc
     4081 gttccagaac attgagatca caatccctag aaacctgctg atcgtaggca aaccggctgc
     4141 ccctcgcgcc tcttgccaat cctacgccgc ggtatccgtg aggtactcca tcgccgtctc
     4201 ccgggagtcg aagatcttga agacggtgtc gagcttggtg atcttgagga gcgccaggat
     4261 gcggtgcgac ggtttgacca gcgccatctt gcgctggcgg tcttcgaact tctgcaggta
     4321 gccgatcagc tcgccgagac cggtcgagtc catgtagttg atcgcttcga agttgatcat
     4381 caccgggccg gtctcatcgt tgaggacctt ctcgaggtag ctcgagaact cgcgcgcgct
     4441 ctcccccagc ttgatcacgc cctggatgtc gagcactgtc acgtcgtcaa cgcggttctt
     4501 gatgatgttc aaagcaaagc tcccttttcg aatcggcgat ggccctagtg tacgggattg
     4561 gcttccgcgt gtcagctcgc ctgaactcag cgctccttct ccggcttgcg gccgatctga
     4621 ttcgtgaccc acgagatgat ggccagcacg atggcgccga tgagggcggc cacgaagtcg
     4681 tcgatgcgga atccggggga aagccacgcc gtcagctcga gcatgaccgc gttgaggacg
     4741 atgagaaaga gtccgagcgt gacgatcgtc agcggaaggg tgagaaagac gaggatgggc
     4801 cggacgatag cgttgagcaa gccgaggatc agggcggcga tggcgagcga gacgatcgat
     4861 cggtagtgaa accccggcac gaccgtgacg acgacgaaga gagcgagcgt gttcagtgcc
     4921 cagcgcagga tcagatgcat ggggacatta tctcccggag aagcacagac aggagtgtct
     4981 gtgccacatc aagcccgttt cacaagtact tgcctcatgg aaagcactat gtggcacaga
     5041 cactcttgtc tgtgctctca cgacatatgc acaacgatct tccttctcgc cagttcctgc
     5101 atgaacagct ccggcgggat gcagcgctcc tgcgggaggg cgcctttttg cgttgtctgg
     5161 ccgcgggcca tcatcagggc aacgatcgac gcggggaacg cggtcgtgcg catcattgcg
     5221 gaaaggccgg tcgcttcgtc gtagcggtcg atgatgtcgt agcggagccg tctcccctct
     5281 cccacgaatt cgacacggac caggacgacg tccggctcat c
//
LOCUS       DATLVW010000483        10893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_556544, whole genome shotgun sequence.
ACCESSION   DATLVW010000483 DATLVW010000000
VERSION     DATLVW010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556544"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..823
                     /locus_tag="VNN25_14530"
     CDS             56..823
                     /locus_tag="VNN25_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92796.1"
                     /translation="MSRPVERYNQLLDQIIIPFDLTHDFGRKRAYEAAIDYARTHLVE
                     DPHCNDFLTAAAKRLGFAAVNFDTLESRVKLPKNAWLDVWISDEDEEVAFTGTREGLQ
                     YMIDLLTQLRDSGDPEQHIHLDQGVSPMTESSANLVLFKEEETWFTGEGEEGDEGGYP
                     EREIDPKQIYAIQFIHYPPDDLPISAHRLYRVIKVEPDDGYSASVKEFGGAENRYFRF
                     TFVADTDEKFTYSFHLDDPAVNYFTHREILKLAMRAV"
     gene            835..1698
                     /locus_tag="VNN25_14535"
     CDS             835..1698
                     /locus_tag="VNN25_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXH92797.1"
                     /translation="MTPNTIRQIAVIGTGTMGRGIAYLAAVAGYETVIHDADAAALDA
                     AKASIESILQKGIERGKVTSDAAGAASRRLHLAPDLEPAVHAADLIIEATPEDIDLKK
                     NLFAQADLFCADETIIASNTSSISISLLAQSVERRDRFVGLHFFNPPHVMKLIEVVRG
                     ERTSDETVEQVTAVAERMGKTAIVVRDSPGFATSRLGVAIGLEAIRMLEDGVASAEDI
                     DKAMELGYNHPMGPLRLTDLVGLDVRLGIAEYLCSTLGLRFDPPELLRRMVADGKLGK
                     KTGEGFYRWSE"
     gene            1695..3728
                     /locus_tag="VNN25_14540"
     CDS             1695..3728
                     /locus_tag="VNN25_14540"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HXH92798.1"
                     /translation="MTTGIEGRRADLRWLSAVVVFIASFALYLKTLAPSVGPTDSGEL
                     TTAVWSLGNAHPPGFPFFLILTHLFTWLPFGSIAVRTNIGTAFFAAVACALVSLAAGE
                     ILMMPRDVRVAKAAAEEKRSKKRQRLDRGQRTAAPAIVAAQVRGPLSMGLVALIMLTT
                     GLLFAVSRTLWQFATVTEVYAVNSALMAGVAWGMLRWARTLNKRWLYAAALLFGLGLC
                     VHHVTIGTGAIAIAVLITRTAGGWFWRSRTVIVAAVLLAAGLLVYIYLPIAASRKPVM
                     NWGDPTTVSAVWDHVTGKQYRGYINTSEEQNSAQLGRYFGIVGRELGPPWVPLVLIIA
                     LGGVALLWFRQRTIFWYVVLGITGNAAWFAIYPITNDTPAYAIPTFIALLFAFAFAAS
                     SIVELLQSERARRIAALGMLILPLISLLATYSIRDRSRYWVSHDYADNALHSMGPNAL
                     MITGDWQLYSPMLYAMDVEHARPDVAVIHAGFLLRPWYFRQMTRRYPVVAADSRRELD
                     ALAAMTPQFNNDRAHLDDELFNRRLDDAVISLIEQHLRRGPVYVTGEIAATNNPRDAN
                     LRKRIYDKWDIVPRGLLIELLPGHKLRSDVTFRPLQTRGVSDGTIEYEDDDVVPTEIA
                     PAYRTPYLMAARYLAIARRFDEALPAYRAALALDPTNSMIEREMRAVAGRAGK"
     gene            3725..4459
                     /locus_tag="VNN25_14545"
     CDS             3725..4459
                     /locus_tag="VNN25_14545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH92799.1"
                     /translation="MTAAESYGAFAYAYDQALGQRYFRAVRRLLAEVLQRYPAAGKTH
                     LDVACGTGLAIPFFESRGFVSAGVDLSTVMLRVARSRAARLIAGDTRALPLRGAFSRI
                     TCLYDSLNHLLEPSDLALAFGEIARVMDDRSQFLFDMNHPEIYPEVWGMKEPFVDEGP
                     DFHLEIATTYRSREKLGRALVTGWAIVRGERVVIRERHTQRCYERDEIIAALAAGGLE
                     PVEEHEFDPWFEGRTVKLFFVCKKKA"
     gene            complement(4675..5241)
                     /locus_tag="VNN25_14550"
     CDS             complement(4675..5241)
                     /locus_tag="VNN25_14550"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HXH92800.1"
                     /translation="MRPKMMSLLLSLTLLVPAFAFAASLADVSLDDKVVVNGQTLSLN
                     GIGLRKKFFIKVYVGGLYLTAKSSNPATILAADSPRRMVMHFLYGVSKDQMCDAWSEG
                     LEANTPNASAEVKNAFKTLCSWMEPIPKGNRLVLTYVPGTGTTVEVNGKAKGTLPGKA
                     TADAIVSTWIGPKPAMGDDFKNAILGKP"
     gene            5343..6329
                     /gene="dusB"
                     /locus_tag="VNN25_14555"
     CDS             5343..6329
                     /gene="dusB"
                     /locus_tag="VNN25_14555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXH92801.1"
                     /translation="MTSLNKPFHIGGVLIDPPLALAPMAEVTDTYYRSLVKELGGAGL
                     VVSEFISAEGLTRDNVRTKAMLKFDEKERPVAIQIYGGDPDRMDDAAAIVEAEGVDIV
                     DVNMGCPVRKVVNSGAGSALLKDFDRAARVVEKIKKRVKIPVTVKVRKGWESDDVLPL
                     LQRFEEIGVSSIAVHGRTRNEAYTGASDWDYIARVKSELGIPVFGNGDVKTAADAIRM
                     FETTGVDGVMIGRAALHNPFIFRDIQALILGVPTDKDEVDRRIDAMHRYLAKIEDAPQ
                     IINWKLHKARTVIGWFSKGIAHGKDIRNGLADITSVADVRRLLDQARQRLAA"
     gene            6447..7604
                     /locus_tag="VNN25_14560"
     CDS             6447..7604
                     /locus_tag="VNN25_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92802.1"
                     /translation="MNTATDVRRSAAAVALAAFLLYGTFVFVQLRRHDFNPTIFVTAG
                     DEFTDRTAPGLLVAPNSSGYDGQFYYRIACDPFSRQPVAAGVHFDYPVYRLQRIGYPF
                     LVWMLTLGNRAAVPWMMIFVNLLSLAGIGAAAVITTRELRVPAWSCLALALYPGFLLS
                     VSRDLVEPLEVLLMMIALVLLMRQRLVLAACVLALAALTKETSLLFALGALVAAIATM
                     PRRRMNFVLLVPFSVHFGWKVLLFRLWHLPMTFVTGEHFMLPFRGFLVRAREAASTGA
                     AGRVVLIEMAAVVVFGIIALLALRSSLAPNAIKASWLLYAALVFLLGTEFWWEDWAFL
                     ASPSNSPSSAASSRLRRPVGASPRSRCSPRDGSLWRVTFFSTAAEKRDALP"
     gene            7591..8526
                     /locus_tag="VNN25_14565"
     CDS             7591..8526
                     /locus_tag="VNN25_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92803.1"
                     /translation="MPFPEHIERIFESFGIRAETKAALFDLFISMGDEVLEVFGDIAE
                     SVADPTMLQPEDCATIRRRLVERYMIRNHPKWIEGKPTPSFYRPRLLEGRAAGVAIPL
                     GGVPANAAGMLDRQPVPDGIVVQGRNAHFGGRSETISFDVIPAVLEDAVALGQALGRQ
                     HTLPGSAGATSGTTDARQQIALLWEIQPNVYKPLGPRNREIAKVYRRHRNWHVITLLA
                     AIEWLRAKSFRVFILRGEALPATHEVDTAKPVTPAIIALHDRTVESVTRALGLPLSPA
                     TPDDEELLANSNVMNTALAKHVAAFGAANAIWRID"
     gene            complement(8599..9141)
                     /locus_tag="VNN25_14570"
     CDS             complement(8599..9141)
                     /locus_tag="VNN25_14570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92804.1"
                     /translation="MSTESFLIRVATRADIESIAAVMRESLLSLGRNAYDERQIASAL
                     LFIARPDEQLIDDGTYFVAIEDEEIVGCGGWSRRRKVYSGSAAVGDESALIDPATEPA
                     RIRAMFVRPECARRGIGRRILQHSEERAAEEGFRNFQLVAMHSAGGIYEAAGYRSISE
                     SPVTLEDGVVLDCTLMEKRL"
     gene            9391..10845
                     /locus_tag="VNN25_14575"
     CDS             9391..10845
                     /locus_tag="VNN25_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH92805.1"
                     /translation="MLFDPSMRALAHDAVDALFDHVGDIARRPVVDWRSAEELRERVR
                     IDDRGGAAPMPLVRMLMENAIQLHHPAYMGHQVCPPFPTAVIADLVISTLNQSTAVFE
                     MSPIGTVIEQEVIRWLADRAGYPATALGTAVSGGSAANLTGLMAARARWNSTRESGAR
                     SDVGRTLSPTNESSRTGESDLRKTLRPIILCSADAHYSIARAASIMGIAAEDVIKVPT
                     NDRHQLDLGAVAAVLHDTGNVMAIVATSGSTATGAFDDLRAIAALRDQYDTWLHVDAA
                     HGASVLLSERLRHLVDGLDLADSLAWDPHKMLWMPLSLGVILVRDGVWLRRAFEADAP
                     YLFNAERASDNIGEVTIQCSKRADAVKLWLTLQVSGIAPFASALDHVTDVTAYLYDRV
                     IESDDFEAMHVPEFNIFCFRHRSDDDTNAALRETLIRSGHAWITSTLLKGQRVLRVTM
                     INPRTERSDVDRMLAALRKIAAQGVAGRESGVGE"
BASE COUNT         1929 a         3406 c         3432 g         2126 t
ORIGIN      
        1 accccccaag gaaatatgat agatttcaag gcgttgtcag ggtgacctaa cgcctatgtc
       61 acgacctgtc gagcgataca accagctcct cgaccaaatc atcatcccct tcgatctaac
      121 gcatgatttc ggacgcaaac gcgcgtacga ggcggcgatc gactacgcgc ggacgcatct
      181 ggtcgaggat cctcactgca acgatttcct caccgcggct gccaagcgcc tcgggttcgc
      241 agcagtgaat ttcgacacgc tcgaaagccg ggtgaagctc ccgaagaatg cctggctcga
      301 cgtctggatc tcggacgaag acgaggaagt tgccttcacc ggtacgcgcg aaggcctgca
      361 gtacatgatc gaccttctca cgcagcttcg cgattccggc gatccggagc agcatattca
      421 cctcgatcag ggcgtctcgc cgatgaccga gagctcggcc aacctcgtgc tcttcaaaga
      481 ggaagagacg tggttcacgg gcgagggaga agaaggggac gagggtggtt acccggagcg
      541 cgagatcgat ccgaagcaga tctacgcgat tcagttcatc cactatccgc ccgacgatct
      601 tccgatcagc gcgcaccgcc tctaccgcgt catcaaggtt gagcccgatg acggctactc
      661 cgcgagcgtc aaagagttcg gcggtgcgga gaaccgctac ttccgcttta cgtttgtggc
      721 cgataccgac gagaagttca cctacagctt ccacctcgac gatcccgccg tgaactactt
      781 cacgcatcgc gagatcctga agctggccat gcgcgcggtg tagactccgc ggccatgacg
      841 ccgaatacga tccggcagat cgcagtcatc ggcaccggca cgatggggcg ggggatcgcg
      901 tatctcgcgg cggtcgccgg ttacgagacc gtcattcacg atgcggatgc cgccgcgctc
      961 gatgctgcga aagcgtcgat cgaatcgatt cttcaaaagg ggatcgagcg cgggaaggtg
     1021 accagcgacg ctgccggcgc ggcgagccgc cgccttcatc tcgctcccga cctcgagccc
     1081 gcggtgcatg ccgccgatct gatcatcgag gcgacccccg aggatatcga tctgaagaag
     1141 aaccttttcg cgcaggcgga tcttttctgc gcggacgaga cgatcatcgc ctcgaacaca
     1201 tcgtcgatct cgatttcgct tctagcgcaa tcggtggagc gccgcgatcg attcgtaggg
     1261 ctgcattttt tcaatccgcc gcacgtgatg aagctgatcg aggtcgtgcg cggcgagcgg
     1321 acgtcggatg agacagtgga gcaggtgacc gcggtggcgg agaggatggg gaagacggcg
     1381 atcgtcgtcc gcgattcgcc cggcttcgcg acctcacgtc tcggcgtcgc catcggtctc
     1441 gaagcgatcc gcatgctgga ggacggcgtt gcctcggcgg aagacatcga taaggcgatg
     1501 gagctcggct acaaccatcc gatgggtcca ttgcggctca ccgatctcgt cggtctcgac
     1561 gtgcgcctcg gcatcgcgga gtacctctgc tccacgcttg gtctgcgctt cgatccccct
     1621 gaactgctgc ggcggatggt cgccgacggt aagctcggca agaagacggg cgaggggttc
     1681 taccgttggt cggagtgacg acgggtatcg agggtcggag ggcggacctg cgctggctgt
     1741 ccgcggtcgt cgttttcatc gcctcgttcg cgctctatct gaaaacactc gcgccttccg
     1801 tcgggccgac ggacagcggc gagctgacga ctgcagtctg gagccttggc aacgcacatc
     1861 cgcccggctt cccatttttc ctgatcctca cgcatctctt cacatggtta ccgttcggtt
     1921 cgatcgcggt gcggacgaac atcgggacgg cgtttttcgc cgcggtggcg tgcgcgctgg
     1981 tatcgctcgc tgcgggcgag atcctgatga tgccgcgcga tgtgcgcgtg gcgaaggcgg
     2041 cagccgaaga gaagcggtca aagaaacggc agcggcttga tcgcggacaa cgaacggcgg
     2101 ctccggcgat cgtggcagca caagtccggg ggccgctgtc gatgggtctc gtcgcgctga
     2161 tcatgctgac gacggggctg ctgtttgcgg tttcgcggac gctctggcaa ttcgcgaccg
     2221 tcaccgaggt ctacgccgtc aacagcgcgc tgatggcggg agtcgcatgg gggatgttgc
     2281 gatgggcgcg gacgctgaac aagcgctggc tctacgccgc ggcgcttctc ttcggactcg
     2341 gtctgtgcgt acatcacgtc acgatcggta cgggcgcgat cgcgattgcc gtgctgatca
     2401 cgcgcaccgc cggcggctgg ttctggcggt cacggaccgt gatcgtcgcg gcggtgctcc
     2461 tcgcggccgg attgctcgtc tacatctacc tgccgattgc ggcatcgcga aagccggtga
     2521 tgaactgggg agatccaacc accgtttccg cggtctggga tcacgtgacc ggaaagcaat
     2581 accgcggcta catcaatacg tcggaagagc agaatagcgc gcagctgggc cgctacttcg
     2641 gaatcgtcgg acgcgagctc ggacctccct gggtgccgtt ggtgttgatc atcgccctcg
     2701 gcggcgtagc gctgctgtgg ttccggcagc gaacgatctt ctggtacgtc gttctcggca
     2761 tcaccggcaa cgcagcgtgg tttgcgatct acccgattac gaacgacacg cccgcgtacg
     2821 ccatcccgac gttcatcgcg ctgctgtttg cgtttgcgtt tgccgcatcg tccatcgtcg
     2881 agttgctgca gagcgaacgt gcgcggcgca ttgcagcgct cgggatgctg atcctcccgc
     2941 tgatttcgct gctcgcgacg tattcgattc gtgaccgcag ccgttactgg gtgtcgcacg
     3001 attacgcgga caacgcgttg cattcgatgg gaccgaacgc gcttatgatc accggcgact
     3061 ggcagcttta ttcgccgatg ctctacgcca tggacgtcga gcatgcgcgg ccggacgtgg
     3121 ccgtcatcca cgccggattc ctgctgcgcc cctggtactt tcgtcagatg acgcgccgct
     3181 atccggtggt cgccgccgat tcgcgccgtg agctcgatgc gctcgcggcg atgaccccgc
     3241 agttcaacaa cgaccgggcc cacctcgacg acgagctgtt caaccgccgc ctcgacgatg
     3301 cggtgatctc gctcatcgag cagcatcttc gccgagggcc ggtctacgtt acaggcgaga
     3361 tcgccgccac aaacaatccg cgcgatgcga atctgcgcaa gcgcatctac gacaagtggg
     3421 acatcgttcc gcgcggtctg ctcatcgaat tgctgcccgg acacaaattg cgttccgatg
     3481 tgacgttcag gccactgcag acccgtggcg tgtccgacgg cacgatcgaa tatgaggatg
     3541 acgacgtcgt tcccacggag atcgcgcccg catatcggac gccgtacctg atggcggcgc
     3601 ggtacctcgc catcgccagg cggttcgacg aggcattgcc tgcataccgc gcggcgctgg
     3661 ctctcgatcc gacgaactcg atgatcgagc gggagatgcg ggcagtcgcg ggccgggctg
     3721 ggaagtgacc gccgccgaat cttacggtgc gttcgcttac gcgtacgatc aggcgcttgg
     3781 tcagagatac tttcgcgcgg tacgtcggct gctcgccgag gtgctgcagc gttatccggc
     3841 cgcggggaag acgcatcttg atgtcgcctg cggcaccggg ctggcgatcc cgttttttga
     3901 gagccgcgga ttcgtgtcgg ctggcgtcga cctctccacc gtcatgctgc gagtggctcg
     3961 cagccgcgcc gcgcgcctga tcgctggcga cacgcgggcg ctgccgctgc gcggggcgtt
     4021 ctcgcgaatc acttgccttt acgattcgct caatcatctg ctggagccat ctgatctcgc
     4081 gctggcgttt ggggagatcg cgcgggtgat ggacgaccga tcgcaattcc tcttcgacat
     4141 gaaccacccg gagatctatc ccgaggtctg gggtatgaag gagccgttcg tcgacgaagg
     4201 accggacttt catctcgaga tcgccacgac ctatcgcagc cgcgagaagc tcggccgtgc
     4261 gctcgtcact ggctgggcga tcgtccgcgg cgagcgcgtc gtcatccgcg agcgccatac
     4321 gcagcgttgc tacgagcgcg acgagatcat cgccgcactg gccgcgggtg gattagagcc
     4381 ggtagaagag cacgagttcg atccctggtt cgaggggaga acggtgaagc tgttcttcgt
     4441 gtgcaagaag aaggcgtagc gccgtacgaa cgtagcgcca ccggctcggt ggctgtatcg
     4501 ccagcggctc gctggcggtt tgcttcattt ctcagcgctg cctgattgcc ggcgacgcgc
     4561 cagattcatg cgccccctca tccgccttcg gcaccttctc ccccactgaa aagcgtgggg
     4621 gagaaggcgc tcgatttgag gttagcgcta gtttacttag ttagccgagg agattcacgg
     4681 cttccctaag atcgcgttct tgaagtcgtc gcccattgcg ggcttcgggc cgatccaggt
     4741 cgacacgatc gcgtcggcgg ttgctttacc gggcagcgtg ccctttgcct tgccgttgac
     4801 ttcgaccgtc gtgccagtgc cgggcacgta cgtgaggacg aggcggttgc ctttcgggat
     4861 tggttccatc cacgagcaca gcgttttgaa cgcgttcttc acttcggcgg acgcattcgg
     4921 cgtgttcgct tcgaggcctt cgctccacgc atcgcacatc tggtctttgc tgacgccata
     4981 gaggaagtgc atcaccatgc ggcgcggtga gtcggcggcc agaatggtgg ctggattgga
     5041 cgatttcgcc gtgaggtaga ggcctccgac gtagaccttg atgaagaact tcttgcgcag
     5101 cccgatgccg ttgagcgaga gcgtctggcc gttgacgacg actttatcgt caaggctgac
     5161 gtcggcgagc gacgcggcga aggcgaaagc cggtacgaga agcgtgagcg aaagtagcag
     5221 agacatcatt ttcgggcgca tgaaaagcct ccattcgttt tgttcgttgg tgtgggatcg
     5281 gcagcatcgt agcaccggga ggtggaagag gcttccggtc cggcgcgttg caaagcattc
     5341 tgatgacgtc cctgaacaag ccgtttcaca ttggcggagt cctgatcgat ccgccgcttg
     5401 cgctggcccc gatggccgag gtgacggata cgtactatcg ctcgctggtg aaggagcttg
     5461 gcggagcggg actggtcgtc tccgagttca tctccgccga ggggctgacg cgcgacaacg
     5521 tacggacgaa ggcgatgctc aagttcgacg agaaggaacg gccggtagcc atccagatct
     5581 acggcggcga tcctgatcgg atggatgatg ctgctgcgat cgtcgaagcc gagggcgtcg
     5641 atatcgtcga tgtgaacatg ggctgtcccg tgcgaaaggt cgtcaacagc ggcgccggct
     5701 ccgcgctgtt gaaggatttc gaccgcgccg cgcgcgtcgt cgagaagatc aagaagcgcg
     5761 tcaagattcc ggtcacggtg aaagtgcgca aagggtggga gagcgacgac gttctgccgc
     5821 tgctgcagcg cttcgaagag atcggcgtgt cttcgatcgc ggttcacggc cggacgcgca
     5881 atgaggcgta cacaggtgcg agcgattggg attacatcgc ccgcgtcaag agcgaattgg
     5941 ggatcccggt cttcggcaac ggcgacgtca agacggcggc ggatgccatc cgcatgttcg
     6001 agacgacggg cgtcgacggc gtgatgatcg gccgcgcggc gttgcacaat ccgttcatct
     6061 tccgcgacat ccaggccctc atcctcggcg ttcccaccga caaggacgaa gtggatcgcc
     6121 gcatcgacgc gatgcaccgc tacctcgcca agatcgagga cgcgccgcag atcatcaatt
     6181 ggaagctgca caaagcgcgc accgtgatcg gctggttctc aaaaggaatc gcgcacggca
     6241 aagacatccg caacggcttg gcggacatca ccagcgtggc cgacgtgcgg cgcctgctgg
     6301 atcaggcacg tcaacgtctg gcggcatagg cccggttctc tggaatcatc gccaccccct
     6361 gaccatcgag cgccttctcc ccccgtgctt ttcgggggga gaaggtggcg aagccgtaca
     6421 aggggggatt gcccgacaat accccagtga acacggctac agacgtccga aggagcgccg
     6481 cagccgtcgc tctcgcagcc ttcctcctct acggcacctt cgtcttcgtc caactccgcc
     6541 gccacgattt caacccgacg atcttcgtga ccgccggcga cgagttcact gatcggacgg
     6601 cgcccggctt gctcgttgcg ccaaactcct ccggctacga cggccagttc tactaccgca
     6661 tcgcctgcga tccgttctcg cggcagcccg tcgccgcggg agtccatttc gactatcccg
     6721 tttatcggct gcaacgaatc ggctatccgt tcctcgtctg gatgctcacg ctcggtaatc
     6781 gcgcggcggt gccgtggatg atgatcttcg tcaacctgtt gtcgctggcc ggaatcggag
     6841 cggcagcggt gatcacgacg cgcgagctgc gcgtgccggc ctggagctgc ctggcgcttg
     6901 cgctctatcc cggctttctc ctctcggttt cgcgcgatct ggtcgaaccg ctggaagtgt
     6961 tgttgatgat gatcgcactt gtactactga tgcggcagcg gctcgtgctc gccgcgtgcg
     7021 ttctcgcgct cgcagcgctg acgaaggaga cgagcctgct gttcgcactc ggcgcgctcg
     7081 tggcggcgat cgcgacgatg ccgcggcgtc gtatgaactt cgtgctgctc gtcccattct
     7141 ccgtgcactt tggctggaag gtgcttctct ttcgcttgtg gcacttgccg atgacgttcg
     7201 tcactggcga gcacttcatg ctgcccttcc gcggttttct cgtgcgtgcg cgagaggcgg
     7261 catcgacggg cgccgcaggc agggtcgttc tgatcgagat ggcagcggta gtcgttttcg
     7321 gcatcatcgc cttgctcgca ctccgctcat cgctcgcgcc aaacgcgatc aaggcttcct
     7381 ggctgcttta cgccgctctc gtcttcctgc tcggcacgga gttctggtgg gaggactggg
     7441 cattcctcgc gtcgccgtcg aattcaccat cctcggcggc atcatcgcgc ttgcgtcgcc
     7501 ccgttggcgc gtcgccgcgc agtcgttgct cgccgcggga tggatcgctc tggcgtgtaa
     7561 cgttcttttc taccgcggct gaaaagagag atgcccttcc ctgagcacat cgagcgaatc
     7621 ttcgaatcct tcggcatccg cgcggagacg aaggcggcgt tgttcgacct cttcatctcg
     7681 atgggggatg aagtgctgga ggtcttcggc gacatcgccg aatcggtcgc cgatccgacg
     7741 atgctccagc ccgaagattg cgcgacgatc cgtcgccggc tggtggagcg ttatatgatc
     7801 cgcaatcatc cgaagtggat cgaagggaag cctacgccga gtttctaccg ccctcgcctg
     7861 ctcgagggac gtgccgcggg cgtggccatc ccccttggcg gcgtgccggc gaacgctgcc
     7921 gggatgctcg accgccagcc ggttccggac gggatcgtgg tgcaggggcg caatgctcat
     7981 ttcggtggcc gaagcgagac catctccttc gatgtgatcc ccgcggttct ggaggatgcc
     8041 gtcgcgctcg gtcaggcgtt ggggcggcaa cacacgcttc ccggatcggc gggagcaacg
     8101 agcggaacga cggatgcgcg acagcaaatc gcgctgttgt gggagattca gccgaacgtc
     8161 tacaagcccc tcggcccgcg caaccgcgag atcgcgaagg tctaccgccg ccaccgcaac
     8221 tggcacgtca tcacgctcct cgcggccatc gaatggctgc gcgcgaagtc gtttcgcgta
     8281 ttcatccttc gcggcgaagc gctgcccgcc acacacgagg tcgataccgc gaagccggtc
     8341 acgccggcga tcatcgcgct gcacgaccgt accgtggaga gcgtgacgcg cgcgctcggt
     8401 ctgccgctct cgcccgcgac gcccgatgac gaagagctgc tcgcaaactc caacgtcatg
     8461 aacactgcgc tggccaagca cgtggccgca ttcggcgcgg cgaatgcgat ctggcgaatc
     8521 gattaggaac cacagagaca cagaggtcac agagaaaggc agtaactctg tgacctctgt
     8581 gtctctgtgg tcaaaggttc agagcctctt ctccatcaac gtacagtcga gcaccacgcc
     8641 atcttcgagc gttaccggcg actcgctgat cgatcgatat ccggcggcct catagattcc
     8701 ccctgcgctg tgcatcgcga cgagttgaaa gtttcggaag ccttcctccg ccgcacgctc
     8761 ttccgaatgc tgaagaatgc gtctgccgat gccacgacgc gcgcactccg gccgcacgaa
     8821 catcgcccgg atgcgtgccg gctccgtggc gggatcgatc agcgcggact cgtcgcccac
     8881 ggccgcgctg ccgctgtaga ccttgcgccg gcggctccat cctccgcagc cgacgatctc
     8941 ctcatcctcg atggcgacaa aatacgttcc gtcgtcaatg agctgctcgt ccgggcgtgc
     9001 aatgaacagc aatgcgctgg cgatctggcg ctcatcgtaa gcgttgcgtc cgagcgacag
     9061 caacgactcg cgcatgaccg cggcaatgct ctcgatgtcg gcacgagtgg caacgcgaat
     9121 gaggaatgat tccgtagaca cggcgccgca gaatacccgt gcacccctcc ctgatcaacg
     9181 cctgtccgtg agctaacgac gaatttctgg gcgcgacgta aggacttttt cacgcgaagc
     9241 cgcgaaggtc gcgaaggaaa gcatcgcaaa ccttaaacct tcgcgtcttc gcggcttcgc
     9301 gtgagaaatg ggtggcgtgc gaatccagct ctttgatctg cgcaaaccag taacgcagca
     9361 cctgcgcaac cgcgaataca atccgccgcc atgctcttcg atccatccat gcgtgctctt
     9421 gcgcacgacg ctgttgacgc tctcttcgat cacgtcggcg acatcgcgcg gcggccggtc
     9481 gtcgattgga ggagtgcgga ggaactgagg gagcgcgttc gtatcgatga tcgagggggc
     9541 gcggcgccga tgccgctcgt ccgcatgctg atggagaacg ccatccagct gcatcacccg
     9601 gcctacatgg ggcatcaggt ctgcccgccg tttcctaccg cggtcatcgc cgacctggtg
     9661 atctcgacgc tgaatcagtc gaccgcagta ttcgaaatga gtccgatcgg aacggtgatc
     9721 gaacaggaag tcatccgctg gttggccgac cgcgccggct atcccgcaac cgccctcggc
     9781 acagccgtct ccggcggctc ggcggcgaat ctcaccgggt tgatggcagc gcgggcgaga
     9841 tggaattcga cgcgggagtc gggagcacgg agtgacgtag gtcggactct cagtccgaca
     9901 aacgaaagca gtcggactgg agagtccgac ctacgtaaaa cattacgtcc catcattctc
     9961 tgctccgctg atgcccatta ctccatcgcg cgggcggctt cgatcatggg gatcgccgcg
    10021 gaagacgtca tcaaagtccc gacgaacgac cgtcaccagc tcgacctcgg cgcagttgcc
    10081 gcggttctgc acgatacggg caacgtcatg gccatcgtgg cgacctccgg ctcgacggcc
    10141 accggcgcgt tcgacgatct ccgcgcaatc gcggcgttgc gtgaccagta cgacacgtgg
    10201 ctgcacgtcg acgcggcgca tggcgcatcc gtgctgctca gcgagcggct ccgacatctc
    10261 gtcgacggac tcgatctcgc cgactcgctc gcctgggatc cgcacaagat gctctggatg
    10321 ccgctctcgc tcggggtgat tctcgttcgc gacggcgtct ggctgcggcg cgcgttcgaa
    10381 gccgacgcgc cctatctctt caacgccgag cgcgccagcg acaacattgg tgaggtgacg
    10441 attcagtgtt ccaagcgcgc cgacgccgtg aaactgtggc tgacgctgca ggtatccggt
    10501 atcgctccct tcgcgtccgc gctggatcac gtgaccgatg tgacggccta tctctatgac
    10561 cgcgtcatcg aatccgacga cttcgaagcg atgcacgttc cggaattcaa catcttctgt
    10621 ttccggcatc gcagcgatga cgatacgaac gcggcactgc gcgaaacgtt gattcgctcc
    10681 ggccacgcct ggatcacgtc gacgttactt aaagggcagc gcgtgctacg cgtgacaatg
    10741 atcaatccac gcacggagcg cagcgatgtc gatcggatgc tggccgcgtt gcggaagatc
    10801 gctgcgcagg gagtcgctgg tcgggagtcg ggagtcggag aatgagcgtt ccgctcaacc
    10861 agaactcgga ctcgggcgct attttcgacc ccc
//
LOCUS       DATLVW010000484         4284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_474198, whole genome shotgun sequence.
ACCESSION   DATLVW010000484 DATLVW010000000
VERSION     DATLVW010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474198"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1258)
                     /locus_tag="VNN25_14580"
     CDS             complement(218..1258)
                     /locus_tag="VNN25_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HXH92806.1"
                     /translation="MADSEPPQSSIVLYQTEDGRTRIQCRFDQETIWLTQALLADLFQ
                     KDVRTINEHLVNIFEEGELSREATIRKFRIVRSEGARQVAREVEHYSLPAILAVGYRV
                     RSHRGTQFRQWATARLSEFLVKGFAMDDERLRNPPGKGQPDYFDELLERIRDIRSSER
                     RFYQKVLDIYATSVDYTPNTEMSQQFFATVQNKMHWATHGHTAAELIHQRADATQPLM
                     GLRTTRPGKIIRREDVSVAKNYLSEEELQTLNRIVSLYIEFAELQALDRKPMTMCNWV
                     EKLDEFLRVSGRELLDHAGQISAETARAKAEREYERYRALADAQPRYVDVDFERAAKN
                     LQKLPRPRKSKS"
     gene            1463..1861
                     /locus_tag="VNN25_14585"
     CDS             1463..1861
                     /locus_tag="VNN25_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH92807.1"
                     /translation="MDDKGKLILRLTVAVLMAFHGISKLRHGVAWMAGPLGAHHLPVW
                     LAYGVYLAEVVAPILLIVGIFTRPAALVIAFEMTVAIYLVLGMKAFELDRQTGALRGE
                     LQLFYFFTAVAIAVLGSGRFALSKGRGRWD"
     gene            1955..3544
                     /locus_tag="VNN25_14590"
     CDS             1955..3544
                     /locus_tag="VNN25_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXH92808.1"
                     /translation="MDWDYVIIGSGFGGSVSGLRLVEKGYRVVMLEKGRRFRAEDFPK
                     SNWNLRRWLWMPSAGFRGIFKMTFLRHVTVLSGVGVGGGSLVYANTLPTPKDEFFTLP
                     SWGHLADWKKELEPHYATALRMLGAMQYPRDTYSDQVLRDIAKDIGRPDQIVPARVAV
                     YFGEPGKTVPDPYLGGEGPDRTGCIQCGACMTGCRHNAKNTLDKNYLWLAEKRGLRIE
                     TDTEVTWVRELAGGGYRIDATSGTGWFGKRRRTFTARNVIFAGGVLGTVPLLLKLKAS
                     ADGLPRLSDRLGGFVRTNSESLIGVTTRADRDLSEGIAITSVLHTDEHSHLEPVRYGK
                     GSGFFRLLMSPHVPGESAPVRLIRVLVAMLRHPIHFLRAYFVDDWAKRTMILLYMRTL
                     EGHLRLRLGAFGLTTALAEGPAPSAAIPEATELARRVEQKIDGFAGSLLSETILGTPT
                     TAHILGGACMGTTAADGVIDDHHRIFGYDGLYVIDGAAMSANPGVNPSLTITALAERA
                     MGFIPAKERTSGDERRQRVRS"
     gene            complement(3615..>4284)
                     /locus_tag="VNN25_14595"
     CDS             complement(3615..>4284)
                     /locus_tag="VNN25_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92809.1"
                     /translation="RGTYGMLRDAGVIDAALEQQLTTERTRKTLIERVMLAYLWNEEA
                     LDSPRIAHLFDRGLVDDLEVASWFLSSVRGGELTQLHMEKIIAFWDKCVSWAEAQVAV
                     PEKLLSSLASLAWSLSSAEGRQGELLLAVAPFADIVGHATHEFLKELLRLAEVSPAQI
                     AAVLGTMVETYSRTYDYEGVMKALITRLAELGQRPVALQYCERLRHVDGIPELFKELR
                     DAAP"
BASE COUNT          764 a         1353 c         1322 g          845 t
ORIGIN      
        1 cgaacttgaa atcggaactt gcttcccaag ccacgagagc cacggtctcg tcgttggaca
       61 cggcgaggag gtcgaggacg ttgctggtca gcgcgcttgg atccgtccag cgcagacgga
      121 ggacgtagtc ttcctgggga atactcatga cggccttcct cccagttgtg tgaatgggag
      181 gatggtactc gagaatttga agttccattt tggaacatca tgacttggac ttcctcgggc
      241 gcggcaattt ctgaaggttc ttcgcagccc gctcgaagtc aacgtccacg taccgcggtt
      301 gcgcatcggc cagcgcccgg taacgctcgt attcgcgctc ggccttcgct ctggccgttt
      361 ccgctgaaat ctgtccggcg tgatcgagca gctcacgccc ggataccctc aggaactcat
      421 ccagcttctc tacccagttg cacatcgtca tcggcttgcg gtcgagtgcc tggagctcag
      481 caaactcgat gtagaggctc acgatccggt tcagcgtctg cagttcttct tcgctgagat
      541 agttcttcgc caccgaaaca tcttcgcggc ggatgatttt gccgggacgc gtagtccgga
      601 ggcccatcaa cggctgcgtg gcgtcggcgc gctggtggat gagctccgcg gcagtgtggc
      661 cgtgcgtggc ccagtgcatc ttgttctgta cggtggcgaa gaactgctgc gacatctccg
      721 tgttcggcgt gtagtcgacg ctggtcgcgt agatgtccag caccttctgg tagaagcggc
      781 gttccgagga gcggatgtcg cggatgcgct cgaggagctc gtcgaagtaa tccggctgtc
      841 cctttcccgg cgggttccgg agacgctcgt cgtccatggc gaaacccttc accaggaact
      901 cgctcaatcg cgctgtggcc cactgccgga actgcgtgcc gcgatggctg cgcacgcggt
      961 agccgacggc aaggatggcg ggcaggctgt agtgctcgac ctcgcgagcg acctggcgag
     1021 cgccctcaga acgaactatc cggaatttcc ggatagttgc ctcccggctc agctcgccct
     1081 cctcgaagat gttcacaagg tgctcgttga tcgtgcgcac atccttctgg aaaaggtcgg
     1141 ccagcagtgc ctgcgtcagc cagatcgtct cctgatcgaa ccggcattgg atgcgcgtcc
     1201 ggccgtcttc ggtctggtag agaacgatgc tcgattgcgg tggctcgctg tcggccatgg
     1261 gtgacggtct agatagcacc ccaacccgcc gcgtgcaacg caagggtgac acgctcagcg
     1321 ggagtcttgc ctttttcatc gaagatctac agattggaga acgtgttgcg ggcgtcggtt
     1381 ttcggggtcg ggggtcggag aaagtcgtgg cctctcttct ccgactcccc actccccact
     1441 cccgaccccc taggagaacc ttatggacga caaagggaag ctcattctcc gactgaccgt
     1501 cgctgtcctg atggcctttc acggaatctc gaaactgcgg cacggcgtcg cctggatggc
     1561 cggaccgctg ggggcgcatc atctgcccgt atggctcgcg tacggcgtgt acctcgcaga
     1621 agtcgtcgcg ccgattctgc tcatcgtcgg aatcttcacg cggcccgcgg cgctcgtgat
     1681 cgcattcgag atgacggtgg ctatctacct cgtcctcggc atgaaggcgt ttgagctcga
     1741 tcgtcagacc ggagcgctcc gtggcgagct tcagttgttc tacttcttca ccgcggtagc
     1801 catcgccgtc ctcggcagcg gccgctttgc gctgtcgaag ggccgcgggc gctgggactg
     1861 aaaagcctct catccgcaga tgacgcagat gtacacagat aagaccatct cccaatctgc
     1921 gtccatctgc gtaatctgcg gataagctct tcccgtggat tgggactacg tcatcatcgg
     1981 ctccggcttc ggcggcagcg tctccggatt gcggctggtg gagaagggct accgcgtggt
     2041 catgctggag aagggacggc gcttccgtgc cgaggacttc ccgaagtcga actggaacct
     2101 gcgaaggtgg ttgtggatgc cgtccgcggg ctttcgcggc atcttcaaga tgacctttct
     2161 gcgccacgtc acggtgctct ccggcgtggg agtcggtggc ggctcgctcg tttacgccaa
     2221 cacattgccg actccgaagg acgagttctt cacgctgccg tcgtggggtc acctggccga
     2281 ttggaagaag gagctggagc cgcactacgc caccgcactg cggatgttgg gggccatgca
     2341 gtatccgcgc gatacctact cggatcaggt tcttcgcgac atcgccaaag acatcggccg
     2401 gccggatcag atcgtgcccg cgcgtgtggc cgtctatttc ggcgagcccg gcaagaccgt
     2461 gcccgacccg tacctcggtg gcgaaggtcc cgaccgcacc ggctgcatcc agtgcggggc
     2521 ttgcatgacc ggttgccggc acaacgccaa gaacacgctc gacaagaact acctctggct
     2581 ggccgagaag cgcggcctgc gaatcgagac ggacaccgaa gtgacgtggg tgagggagct
     2641 tgccgggggc ggctaccgga tcgatgccac ctcgggcacc ggctggttcg gcaagcggcg
     2701 gcgcacattc accgcgcgca acgtgatctt cgccggcggc gtcctcggca ccgtgccgct
     2761 cctgctgaaa ctgaaggcca gcgcggacgg attgccgcgc ctctccgatc gcctgggcgg
     2821 cttcgtccgc acgaactcgg aatcgttgat cggcgtgacc acgcgcgccg accgcgactt
     2881 gagcgaaggc atcgccatca ccagcgtgct ccacaccgac gagcactccc acctcgagcc
     2941 ggtgcgctac ggaaaaggct ccggcttctt ccggttgctg atgtcgccgc acgtgccggg
     3001 ggaaagcgcg ccggtgcgat tgatccgcgt tctcgtggcg atgctgcggc accccatcca
     3061 cttcctgcgc gcctatttcg tcgacgactg ggcgaagcgg acgatgatcc tgctctacat
     3121 gcgcacgctg gaaggccacc tccgtcttcg cctcggcgcg ttcggcctga cgactgcgct
     3181 ggccgaggga cctgcgccga gcgcggcgat ccccgaggcg acggagttgg cgcggcgggt
     3241 ggaacagaag atcgatggct tcgccggcag cctcctcagc gaaaccatcc tcggcacccc
     3301 caccaccgcc cacatcctcg gcggcgcgtg catgggcacg accgcggccg acggcgtgat
     3361 cgacgaccat caccgcatct ttggctacga cggcctctac gtaatcgacg gcgccgcgat
     3421 gtccgccaac ccaggcgtga atccgtcgct gacgataacg gcgctggcgg agagggcgat
     3481 ggggtttatt ccggcgaagg aaaggacatc cggagatgaa cgcagacaaa gggttcggtc
     3541 gtgaaatgga aagtggcccg acatcagctc accgtggcgc cgtgaggtca aagcgaagac
     3601 gggcacgagc cttctcacgg agcagcgtcg cgcagctcct tgaagagttc gggtattcca
     3661 tctacgtgcc tgagccgctc gcagtactgc agcgcgaccg gtcgctggcc aagttctgcc
     3721 aatcgcgtga tcagcgcctt cataacgcct tcatagtcgt acgttcggct atatgtctcg
     3781 accatcgtgc caagcaccgc cgcaatctga gcagggctaa cttctgcgag cctcaggagc
     3841 tctttgagga attcgtgcgt tgcgtgcccg acaatatcgg cgaacggcgc aacagcgagg
     3901 agtaactctc cttgcctgcc ctccgcgctg ctgagcgacc acgccagaga ggcgagcgat
     3961 gaaaggagtt tctcgggaac agccacttga gcctcagccc agcttacgca tttgtcccaa
     4021 aaggcgatga tcttctccat atgtaattga gtcagctctc caccccgcac gctcgacaga
     4081 aaccagctcg cgacctccag atcatcgacg agccctcgat cgaagagatg cgcaatcctc
     4141 ggggaatcga gcgcttcctc attccaaagg tatgcaagca tgacgcgctc aatcaacgtc
     4201 ttccgtgtgc gttctgtcgt gagctgctgt tcaagagccg cgtcgatcac ccccgcgtcc
     4261 cgtagcatcc cgtaggtacc cctt
//
LOCUS       DATLVW010000485         7849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_556709, whole genome shotgun sequence.
ACCESSION   DATLVW010000485 DATLVW010000000
VERSION     DATLVW010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556709"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VNN25_14600"
     CDS             complement(<1..640)
                     /locus_tag="VNN25_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXH92810.1"
                     /translation="MTVQTPAAPASESAPAYSLNDLLVYMAKQQASDLHLKPMRPPLL
                     RIRGKLVPVKSDPLKPADLEKMLLPLLNRVQKEKFEATQSVDFGYGVAGVARFRANVY
                     MQRGTVGAVFRRIPINVMGIDALELPDAIRDLTQIPDGLVLVTGPTGSGKSTTLASMI
                     DHINATRTDHVITIEDPIEFVHGSKKCLINQREVRLHTESFKRALRAALREDP"
     gene            complement(712..1113)
                     /locus_tag="VNN25_14605"
     CDS             complement(712..1113)
                     /locus_tag="VNN25_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH92811.1"
                     /translation="MTALQPKRIVIVDDSPAYCDLWSNFMTERYAETAHVETYSHPYD
                     ALPKIDDSIDLLIVDLEMPGMDGKKFVDYARAKGVPARRIVVTSSHSADELHRRFRIG
                     ETIAVINKTEAKQQEAFLMILDSVMRRKSEG"
     gene            complement(1110..2414)
                     /locus_tag="VNN25_14610"
     CDS             complement(1110..2414)
                     /locus_tag="VNN25_14610"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXH92812.1"
                     /translation="MNLLITNGRVIDPSQELDAKLDVLIEDGLIARVDKRIKANVEVF
                     DAEGLVVTPGLIDLHTHLREPGQEHKETIATGTRAAVAGGYTAVCAMANTLPPNDERA
                     VTEMIIAESRRNGACRVYPIGAVSKGLMGEALAELADLRAAGCVGVSDDGRPVWNAQL
                     MRRALEYCTMLGIPVIAHEEDANLSDHGVMHEGYFSTLLGLGGIPAASEETLVARDVI
                     LARATGAHVHIAHLSTAGGVDSVRHARAAGVRVTCEVTPHHLALGDENVQSFSTNLKM
                     NPPLRSAEHRQALIDGIADRTIDAIATDHAPHHFDEKNVEFDLAPFGIIGLETAFAVC
                     YDTLVRSGVIALDHLIDLMSCGPARIFNLPGGTLRNGAIGDVTLLDLDAKFTVPNTFR
                     SKASNSPFVGKTFHARPVATVVGGAVRFDGRATKPAKRKGKK"
     gene            complement(2487..3437)
                     /locus_tag="VNN25_14615"
     CDS             complement(2487..3437)
                     /locus_tag="VNN25_14615"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXH92813.1"
                     /translation="MRASTSSLRSKDLLGIEHLLPEEIELILDTAEGFKEVSERPVKK
                     VPALRGQLIINLFMEASTRTRVSFEIAEKRLSADTLNFAASGSSVEKGETLIDTAENL
                     VAMHPEMIVIRHKYPGAPKMLADRLPNVSIINAGDGSHEHPTQALLDAFTIRERLGQL
                     AGVNVSIIGDIAHSRVVRSNIHLLTKMKANVTVAAPPTLLPAEIGKMGVRVVHSLDEA
                     IAGADVIMMLRIQLERQGKLSFPSLREYYNTFGLTPERLKRASSEAIIMHPGPMNRGV
                     EIASDVADDPKRSVILEQVTNGVAVRMAVLYLLGGAHGNG"
     gene            complement(3596..4132)
                     /gene="pyrR"
                     /locus_tag="VNN25_14620"
     CDS             complement(3596..4132)
                     /gene="pyrR"
                     /locus_tag="VNN25_14620"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXH92814.1"
                     /translation="MNVKKRLLDGPRMNRAIRRMAIEVLEKNRGTEDLIIVGIRSRGV
                     PIAERMAKEIEEMEGHPVSSGILDITLYRDDLTTIAPQPVVKPTKLPEPIDDKIVVLV
                     DDVLYTGRTVRAALDALIDFGRPKAVMLAVLIDRGHRELPIHADVIGKTVPTDAHEVI
                     KVKLVETDGEDEVLIMEK"
     gene            4586..5461
                     /locus_tag="VNN25_14625"
     CDS             4586..5461
                     /locus_tag="VNN25_14625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXH92815.1"
                     /translation="MDQRYSTIIFVPHARAKFRKLKVSHRLLFSVVSVLTSSLCLSTF
                     FSVQYFTSLSQTHELSKLRHENKDLQTANEQFSKSVESLRGQLRTVEDRTRKLAIYAG
                     ISSLDDTAQGGVGGLRPTELGDNPYRDDVDKMAFRSRRLEGDLSVLEQKLVAQSQLLA
                     STPSIAPVRGILTDGFGGRSDPFTGEAGTHNAIDISSAVGQPVRAPADGIVVKAEWAN
                     GYGNVIYISHGYGYSTRYGHLSAYAAKPGQHVKRGDIIAYVGSTGRSTGPHLHYEVRL
                     NNNPVNPLEYILNAF"
     gene            complement(5540..6526)
                     /gene="trxB"
                     /locus_tag="VNN25_14630"
     CDS             complement(5540..6526)
                     /gene="trxB"
                     /locus_tag="VNN25_14630"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXH92816.1"
                     /translation="MERIKDYKVIVIGSGPAGCTAAIYLSRANLAPLVLEGLQPGGQL
                     TITTDVENYPGFPEGILGPKLMDLMRQQAERFGAAFKMEQVESVKLTGHPLVITTDSH
                     EYECQALIIAAGASAMQIGLPSEKALQGFGVSYCATCDGFFFKNKEIVVVGGGDSAME
                     EATFLTKFASKVTVIHRRDELRASKIMQDRARGNPKVEFILDTTVDEVIGTKEGGITS
                     LKIRNLKTDAVRDFATQGLFVAIGHTPNTQAYAGQLELDSRGYICLKHPHSTATNIDG
                     VFACGDAVDHVYRQAVTAAGTGCMAALDCEKWLTHERITERWDLPVGEEERE"
     gene            6615..7586
                     /gene="queG"
                     /locus_tag="VNN25_14635"
     CDS             6615..7586
                     /gene="queG"
                     /locus_tag="VNN25_14635"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXH92817.1"
                     /translation="MGNVLLPGKKPMITRLSEVWPAVERIARTNGVIRLGASNITDTH
                     RALFDEWLDLGHHGTMHYLARTRDVRRDPHVRFPWAKSAIVIVVPYASARPGAPPNAL
                     SHSIARYALGDDYHDVLDQILRQIEPVVAPYGKTWRYVDTGPLSDRALATQAGLGWIA
                     KNAMLINEAIGSYTFIGTLLTSLENDLVPDSVADRCGTCTRCIDACPTNAILPNRTVA
                     SEHCISYATIEHRGDLPDFPLAGNAFGCDICQEVCPWNHEPAASHPAFEPRNEYRATP
                     VTDLLRYEQSDFSSLFRKSAVKRAKLAGMQRNIAALTEAESRGMLKP"
     gene            7656..>7849
                     /locus_tag="VNN25_14640"
     CDS             7656..>7849
                     /locus_tag="VNN25_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92818.1"
                     /translation="MFLIPCSGRSAGGNHRRCTSIGILSPISLPNETGTDDSGGLTMT
                     KDITVQELQEHFADHFDEVRR"
BASE COUNT         1444 a         2484 c         2392 g         1529 t
ORIGIN      
        1 cggggtcctc gcgcagcgca gcgcgcagcg cgcgcttgaa gctctcggtg tgcaggcgca
       61 cttcgcgctg gttgatcagg cacttcttgg agccgtgcac gaactcgatc gggtcctcga
      121 tcgtgatcac gtggtcggtg cgcgtggcgt tgatgtggtc gatcatcgag gccagcgtgg
      181 tcgacttgcc cgagccggtc ggaccggtca caagcacgag gccgtccgga atctgcgtga
      241 gatcgcgaat ggcgtccggc agctccagag cgtcgatgcc catcacgttg atggggatgc
      301 gccggaaaac cgcgccgacc gtaccgcgct gcatgtagac gttcgcgcgg aaacgcgcca
      361 cgcctgccac gccgtagccg aagtcgaccg actgcgttgc ctcgaacttt tccttctgca
      421 cgcgattcag caacggcagc agcatcttct cgagatcagc cggtttgagc gggtcgctct
      481 tgaccggcac gagcttgccg cggatccgca gcagcggcgg ccgcatcggc ttgagatgaa
      541 ggtccgacgc ctgctgcttg gccatgtaga ccagcagatc gttcagcgag taggcgggag
      601 cggattccga agcgggagcc gccggcgttt gaaccgtcac ggggtgatta taaggatgaa
      661 ggcggaagga tgaaggatga acttaccaaa ctgtctttca tccttcgtct ctcacccttc
      721 actcttcctt ctcatcacgc tgtcgaggat catcagaaac gcttcctgct gctttgcttc
      781 ggtcttattg atgaccgcga tggtctcgcc gatgcggaag cgccggtgga gctcgtcggc
      841 ggagtgcgat gaggtgacga cgatccgccg cgccggaacg cctttggccc gcgcgtagtc
      901 gacgaacttc ttgccgtcca ttccaggcat ctccaggtcg acgatgagga ggtcgatgct
      961 gtcgtcgatc ttcggaagcg catcgtaggg gtgcgagtag gtctccacgt gcgcggtctc
     1021 ggcgtagcgc tcggtcatga agttcgacca gagatcgcaa tacgcgggcg aatcgtcgac
     1081 gataacgatc ctcttcggct ggagcgcagt catttcttcc ctttccgttt cgccggtttg
     1141 gtcgcgcgcc cgtcaaatcg aacggctccg cccaccacgg tggccaccgg ccgcgcgtgg
     1201 aaggttttcc ccacgaacgg cgagttcgat gctttcgatc ggaaggtgtt ggggaccgtg
     1261 aacttcgcgt cgaggtcgag cagcgtcacg tcgcctatcg cgccgttgcg cagcgtgccg
     1321 cccggcagat tgaagatgcg cgccggaccg cacgacatca gatcgatcag gtgatcgaga
     1381 gcgatcacgc ccgaacgaac aagcgtgtcg taacagacgg caaaggcggt ctccagaccg
     1441 atgatgccga agggcgcgag atcgaactcg acgttctttt cgtcgaagtg atggggcgca
     1501 tgatcggtgg caatagcgtc gatagtgcgg tcggcaatgc cgtcgatgag ggcctgacga
     1561 tgttccgctg agcgcagcgg cgggttcatc ttcagattcg tcgagaacga ctgaacgttc
     1621 tcgtcgccga gggcgaggtg gtggggggtg acttcgcagg tgacgcgcac gccggccgcg
     1681 cgcgcgtggc gaaccgagtc gacgccgccc gcggtcgaga gatgcgcgat atgcacgtgc
     1741 gctccggtcg cgcgcgccag gatcacatcg cgtgcgacga gtgtctcttc cgaggcggcg
     1801 gggatgccgc cgagtccaag tagtgttgag aagtagccct cgtgcatcac gccgtggtcg
     1861 gagagattgg cgtcctcctc gtgtgcaatc accggaatgc cgagcatggt gcagtactcg
     1921 agtgcgcgcc gcatcaactg cgcgttccag accggccggc cgtcgtcgct gacgccgacg
     1981 cagcccgccg cgcgaaggtc ggccagctcg gccagcgcct cacccatcaa ccctttgctg
     2041 accgcgccga tcggatagac gcggcaggca ccattccgcc gcgactccgc gatgatcatc
     2101 tccgtgaccg cgcgctcgtc gttgggaggg agcgtgttcg ccatcgcgca gacggcggtg
     2161 tagccgccgg cgacggctgc tcgagtgccg gtggcgatgg tctctttgtg ttcctggccc
     2221 ggctcgcgga gatgtgtgtg aaggtcgatc agtcccggag tgacgacgag gccctcggca
     2281 tcgaacacct caacgtttgc tttgatccgt ttgtcgacgc gcgcgatcag gccgtcttcg
     2341 ataaggacgt cgagcttggc atcaagctcc tggctcggat cgatgacgcg gccgttggta
     2401 atcagaaggt tcatttttta tccgcagatg acgcagaggg acgcagatct gtgctcatct
     2461 gcgtcatctg cggatcaaaa gctttttcac ccgttgccat gcgcacctcc gaggagatac
     2521 aaaaccgcca tccgcaccgc cacaccattc gtcacctgct cgaggatcac tgaccgcttg
     2581 ggatcgtcgg cgacatcgct ggcgatctcc acgccgcggt tcatcgggcc gggatgcatg
     2641 atgatcgcct cgctgctggc gcgtttgagc cgctccggcg tcaggccgaa cgtgttgtag
     2701 tactcgcgca gcgacggaaa cgacagcttc ccctgccgct cgagctggat gcgcagcatc
     2761 atgatgacgt cggcgccggc gatggcttcg tcgagcgagt ggacgacgcg cacacccatc
     2821 tttccgatct cggccggcag cagcgtcggc ggcgcagcaa ccgtcacgtt cgccttcatc
     2881 ttcgtcagca ggtgaatgtt cgaccggacc acgcgcgagt gcgcgatgtc gccgataatc
     2941 gagacattga cgccggcaag ctggccaagg cgctcgcgga tcgtgaaggc gtcgaggagc
     3001 gcctgggtgg gatgttcgtg cgaaccgtcg ccggcgttga tgatcgagac attcggcagg
     3061 cgatcggcca gcatcttcgg cgcgccggga tacttgtgcc gaatgacgat catctccgga
     3121 tgcatggcca ccaggttctc ggccgtgtcg atgagcgtct cccctttttc caccgacgag
     3181 ccggaagcgg cgaagttgag cgtatcggcg gaaaggcgtt tctcggcgat ctcgaacgag
     3241 acccgcgtcc gcgtcgaagc ctccatgaaa aggttgatga tgagctgccc gcgcaaggcc
     3301 ggcactttct tcaccggccg ctcgctcact tccttgaacc cctccgccgt atcgagaatc
     3361 aactcgatct cctcggggag gagatgctcg atgccgagaa ggtccttgga gcggagcgag
     3421 gaggtggagg cgcgcatgag gcagtggatt agtagattag tggatcagcc gacaaggatc
     3481 gctgatgttt ctgagatcag agacaagaga tcagagatgc aatctgatct cttatctctt
     3541 gtcacttgtc tccctttctc tgcagcttct gatcactaat cagctaaccg ccgatctact
     3601 tctccatgat caaaacctca tcctcgccat ccgtctccac cagcttcacc ttgatgactt
     3661 catgcgcgtc ggtggggacc gtcttgccga tcacgtcggc atggatgggg agctcgcggt
     3721 gaccgcgatc gatgaggacg gcgagcatga ccgccttggg acggccgaag tcgatcagcg
     3781 catcgagcgc cgcgcgaacg gtgcggccgg tgtagagcac gtcgtcgacc aggacgacga
     3841 tcttgtcgtc gatcggctcg gggagtttgg tcggcttcac caccggctgc ggcgcgatcg
     3901 tggtgaggtc gtcacgatag agggtgatgt cgagaatgcc ggagctgacc ggatgccctt
     3961 ccatctcctc gatctctttc gccatccgct cggcgatggg aacgcctctg ctgcgtatcc
     4021 cgacgatgat cagatcctcg gtgccccgat ttttctcgag gacttcaatg gccatgcgcc
     4081 ggatggcgcg attcattcga ggaccgtcga gaagccgctt tttcacgttc atacagacct
     4141 tagttgatcg agatttgccg ccgttcggaa gggcggcggg aggatagcac ggtggtgtgg
     4201 gagtggggag tggggagtgg ggagtgggga gtggggagtg gggagtggcg tgtggcgtgt
     4261 ggtccggaaa accggccaga cgaagcgaag caccggatta ttgaatatcg gaagaaagaa
     4321 gaggggcact cagaacgcct ccggaaccga ttcgctccca ctccccactc cctacccccc
     4381 acaccccacc cgatacttat tagcgttatg gaacggaaac cctaccgcat gcgtcccgaa
     4441 tatgacgtta caccataaaa cgcgtccgga agtgcttgtg ttcacataac ttaacagccg
     4501 tttttgcgtt gacacgcacc gctctttcca tagaatacgc cccctacacc gtcgtccagt
     4561 cggtatcccg agttaaggag tacgcatgga tcaacgctat tcaacaatca tctttgtccc
     4621 gcatgcccgg gccaaattcc ggaagttgaa ggtatcccat cgcctgctct tctccgtcgt
     4681 ctctgtcctg acgtcttccc tctgcctgtc gaccttcttc tctgtccagt acttcacctc
     4741 cctctcgcag acgcacgagc tcagcaaact ccgccacgag aacaaagacc tccagaccgc
     4801 caacgaacag ttcagcaagt ccgtcgaatc gctccgcggc cagctccgta ccgttgaaga
     4861 ccgcacgcgc aaactggcga tctacgccgg catcagcagc ctcgacgaca ctgcccaggg
     4921 cggcgtcggc ggtcttcgcc ccacggagct cggagacaat ccgtaccgcg acgatgtgga
     4981 caagatggcg ttccgctcgc gccggctcga aggggatctg tcggtccttg aacagaagct
     5041 cgttgcacag tcgcagctcc tcgcttccac accatccatc gcaccggtcc gcggcatcct
     5101 gacagacggc ttcggtggcc gctccgatcc gtttacaggc gaagccggca ctcacaacgc
     5161 gatcgacatt tcgtccgcgg tcggccagcc cgttcgcgca cccgcggatg gcatcgtcgt
     5221 caaggccgag tgggccaacg gatacggcaa cgtcatctac atctcccacg gctacggcta
     5281 ctcgacgcgc tacgggcatc tctcggccta cgcggccaag cccggccagc acgtcaagcg
     5341 cggcgacatc atcgcttacg tcggctcgac cggccgctcc accggcccgc accttcacta
     5401 cgaagtgcgc ctgaacaaca acccggtcaa tccgctcgag tacatcctca acgcgttctg
     5461 atattgcgct tcgcgcgttg tcgccgaggc ttagggtcgc gcaagcctca cgagtcacgc
     5521 gccaccggcg cgcaatacat cactcccgct cctcttcccc taccggcaga tcccatcgct
     5581 ccgtgatccg ttcgtgcgtg agccacttct cgcagtcgag ggcggccatg catccggtgc
     5641 cggcggcggt gacggcctgg cgatagacgt ggtcgacggc atcaccgcag gcgaagacgc
     5701 catcgatgtt tgtcgccgtc gagtgcggat gtttcagaca gatgtagcca cgagagtcga
     5761 gctcgagctg cccggcgtag gcttgcgtgt tgggggtgtg accgatggcg acgaaaagac
     5821 cctgcgtcgc gaaatcgcgc actgcgtcgg tcttcaggtt gcggatcttg agcgacgtga
     5881 tgccgccctc tttcgtgccg atgacctcat cgaccgtcgt gtcgagaatg aactcgacct
     5941 tcgggttgcc gcgggcgcgg tcctgcatga tcttcgacgc acgcagttcg tcgcggcgat
     6001 ggatgaccgt cactttcgag gcgaacttcg tgaggaacgt cgcctcctcc atcgccgaat
     6061 cgccgccgcc caccacgacg atctctttgt tcttgaagaa gaatccgtcg cacgtggcgc
     6121 agtaggagac gccgaaaccc tgcagcgcct tttcgctcgg cagtccgatc tgcatggccg
     6181 acgcgcccgc cgcgatgatc aacgcctggc attcgtactc atgcgaatcc gtcgtgatca
     6241 ccagcggatg gccggtgagc ttcacactct caacctgttc cattttgaac gccgcgccga
     6301 agcgctcggc ctgctggcgc atgagatcca tcaacttcgg cccgaggatc ccttccggaa
     6361 atccaggata gttctcgacg tcggtggtga tggtgagttg tccgcccggc tgcaggccct
     6421 cgaggacgag cggggcaaga ttcgcgcggg agagatagat cgctgcggtg catccggccg
     6481 ggccggagcc gatgacgatg actttgtaat ccttgattcg ttccatgggg cgcgcattct
     6541 atcgcgcggg aggcgcgtgt cacctcgggc gacttcgcgc gccatgagcg cgtcgccgca
     6601 ctggatttca ctgaatgggc aacgtgttgc tgcccggcaa gaaaccaatg atcacgagac
     6661 tgtccgaagt ctggcctgcc gtcgagcgca tcgcccgcac caacggcgtc atccgcctcg
     6721 gcgccagcaa catcacagac acgcaccgcg cgctgttcga cgagtggctc gacctcggac
     6781 accacggcac gatgcactat cttgcccgca cgcgcgacgt gcggcgcgat ccgcacgtgc
     6841 gtttcccgtg ggccaagtcg gcgatcgtga tcgtcgtgcc gtacgcgtcg gcacgtccgg
     6901 gcgcgccgcc gaatgccctc tcccactcca tcgcccgcta cgctctcggc gacgactatc
     6961 acgacgtgct cgatcagatc cttcgtcaga tcgagccggt tgttgccccg tacggaaaaa
     7021 catggcgcta cgtcgatacc ggtccgctgt ccgaccgcgc cctggccacg caagcaggcc
     7081 tcggctggat cgccaagaac gccatgctga tcaacgaagc gatcggctcg tacacgttca
     7141 tcgggacgct gctcacatca ctcgaaaacg atctcgtccc cgattccgtc gccgaccgct
     7201 gcggcacctg cacgcgctgc atcgacgcct gcccaacgaa cgcgatcctc cccaaccgca
     7261 ccgtcgcgtc ggagcattgc atcagctacg cgacgatcga gcaccgcggc gatctgcccg
     7321 actttcccct cgccggcaac gccttcggat gcgacatctg ccaggaagtc tgtccgtgga
     7381 accacgagcc cgccgcttcc catcctgcct tcgagccccg caacgagtat cgcgcaacgc
     7441 cggtcacaga tctcctgcgt tacgagcagt ccgatttctc atcgctcttt cgcaagagcg
     7501 ccgtcaaacg ggccaaactg gcaggcatgc aaaggaacat cgcggcgttg acagaggcgg
     7561 aaagccgagg gatgctgaag ccatgacgag ggatgaagag ccggttgcgc gagacatgat
     7621 cgcgatcgag actaacgacg acgatttctt cgttcatgtt cctgatcccg tgctcgggcc
     7681 gctcggcggg tggaaaccac cgtcgctgca cctcgattgg gatcctgtcc ccgatctcgt
     7741 tgccgaacga gaccggtacc gatgattccg gaggattaac catgaccaaa gacatcactg
     7801 tgcaggagtt gcaggagcac tttgctgatc atttcgatga agtgcggcg
//
LOCUS       DATLVW010000486         2791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_19874, whole genome shotgun sequence.
ACCESSION   DATLVW010000486 DATLVW010000000
VERSION     DATLVW010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19874"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VNN25_14645"
     CDS             complement(<1..122)
                     /locus_tag="VNN25_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXH92819.1"
                     /translation="MHTIVIALAAFAAGAINSIAGGGTLLSFPALVWIGVNPVMA"
     gene            complement(346..1866)
                     /gene="glmM"
                     /locus_tag="VNN25_14650"
     CDS             complement(346..1866)
                     /gene="glmM"
                     /locus_tag="VNN25_14650"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXH92820.1"
                     /translation="MRTLFGTDGIRGKAHQYPFDGPTMFALGEALAHRLKGVGSRESG
                     VGATPEVTLRQPTTDNRQPAIGEPSADSLRPPTPDSRLPGSPHPTPHTPHPTILLGMD
                     TRESGPEIARALTAGIEHGGGRAIFIGIVPTPAVAYLCRTSDAAAGISISASHNPFED
                     NGVKIFGHDGMKIPDSVEEQIEDELRALRRDDVVIPDRELRENGELIERYEQFLDDGI
                     APQALRGRKVVLDTGNGATFRIAPEVFRRAGAEVVVINAQPNGRNINDQCGAMHPEGL
                     AKTVVAEKADFGVAFDGDADRAIFVDDVGKIRDGDEIIYLWAQRLKRRGILNNDRVVT
                     TVMSNFGFEQQLHEDGIELCRANVGDKYVLEMMMERGAILGGEQSGHIIDLAVHTTGD
                     GTHTALVFGELLTRAGRPFSELRTFTPMPQLLVNQPVASKPPLESLPKYQAALAVAQA
                     QLNGQGRILVRYSGTENKVRVMVEGPDRALIEQIAESLRVVLESEISGTRGSATRD"
     gene            complement(1863..2636)
                     /locus_tag="VNN25_14655"
     CDS             complement(1863..2636)
                     /locus_tag="VNN25_14655"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HXH92821.1"
                     /translation="MSSHNLSLRILSLFLAFTVWFFVSAPRREPVSERAFAAPVSIVR
                     MPRDLVITTPVPDTVSVRLRGRISDLRSLSSQNLEVTLDVSWVTPGDAVITLSPQAMS
                     VPPQIDVVSIEPMKLRFHVEALRQTVVPIRPFLIGQPPPGYLAGDTTLVPDHALVSGP
                     ASQIRNVTEVATERIIMSGRTETFVQNVGVVSDSSLVRIIEPLVTQVTVPVNAEIGPV
                     APASATTDTTAEEASVEKPAETKPPEKTPVQKRRHKREQ"
BASE COUNT          478 a          813 c          948 g          552 t
ORIGIN      
        1 gccatcactg ggttgacgcc gatccacacg agcgcgggaa aggagagaag tgttcccccg
       61 ccagcgatcg agttgattgc gccggccgcg aaggcagcga gggcgatgac aatggtgtgc
      121 atggactcag agatcaggaa tcagagacaa gagatcaggg ctcagaggca ggacagcatt
      181 tcatattccg ggcggtcgag acgcgcgcct tgggttagga gtgcgggcgt ctccgcccgc
      241 ggccatcgag gcgtcccgcc tcgatggttg gatccggccc gatagctcca tggttctgaa
      301 agacgcttaa cgtcaatcga gagcgcgatt cctgatctca gatctctagt ctctggtcgc
      361 tgatccccga gtcccgctga tctctgattc cagcacgact cgcagcgact cggcgatctg
      421 ttcgatgagc gctcgatcgg ggccttcgac catgacgcgc actttgttct cggtgccgga
      481 gtatcggacg aggatgcggc cctggccatt taactgcgct tgcgcgacag ccagcgccgc
      541 ctggtatttc ggcagcgatt cgagcggcgg tttcgaggcg accggctggt taacgagcaa
      601 ctgcggcatc ggcgtgaatg tgcggagctc ggagaatggc ctgcctgccc gtgtgagcaa
      661 ctcgccgaag acgagagcgg tgtgggtgcc gtcgccggtc gtgtgaaccg cgaggtcgat
      721 gatgtggccg gactgctcgc cgccgaggat cgcgccacgc tccatcatca tctcgaggac
      781 gtacttgtcg ccgacgttgg cgcggcaaag ctcgatgcca tcctcgtgaa gctgctgctc
      841 gaagccgaag ttggacatta cggtcgtaac gacacgatcg ttattgagta tgcctctgcg
      901 cttcaggcgc tgcgcccaga ggtagatgat ctcgtcgccg tcgcgtatct ttcccacgtc
      961 gtcgacgaag atggcacggt cggcgtcgcc gtcgaaggcg acaccgaagt ccgccttctc
     1021 tgcaaccacg gtctttgcca gtccctcggg gtgcatcgca ccacactggt cattgatgtt
     1081 gcggccattc ggctgcgcgt tgatgacaac gacttccgcc ccggcgcgcc ggaagacttc
     1141 gggcgcgatt cggaaggtgg cgccgtttcc tgtgtcgagc acgaccttgc ggccgcgcaa
     1201 cgcctgggga gcgatgccgt catcgaggaa ttgctcgtaa cgctcgatca gctccccgtt
     1261 ctcgcggagc tcccgatccg ggatgacgac gtcgtcgcgg cgaagcgcgc gaagctcgtc
     1321 ctcgatctgt tcttcaacac tgtcgggaat cttcatccca tcgtgcccga agatcttgac
     1381 gccgttatct tcgaagggat tgtgcgaggc cgagatggag atgccggcag cggcatccga
     1441 ggtgcggcaa aggtaggcca ccgcaggggt cggaacgatt ccgatgaaga ttgccctgcc
     1501 gccgccatgc tcgatgccag cagtcaacgc gcgggcgatc tccggaccgg attcgcgtgt
     1561 gtccatgccg aggaggatgg tggggtgtgg ggtgtggggt gtggggtgtg ggcttccggg
     1621 gagtcgggag tcgggagtcg ggggtcggag agagtcagcg gaaggctctc cgattgcggg
     1681 ttgtcggttg tcggttgtcg gttgtcgaag ggtgacctcg ggagttgctc cgactcccga
     1741 ctcccgactc ccgactccct tcagccgatg cgccagcgct tcgccgagcg cgaacattgt
     1801 cggtccgtcg aatgggtact gatgggcttt gccgcggatg ccgtcggtgc cgaataaggt
     1861 cctcattgct cccttttgtg tctgcgtttt tgcaccggcg tcttttctgg cggcttcgtc
     1921 tcggcaggct tttcgacgga cgcctcctcg gcagtcgtgt ccgtggttgc cgatgcaggc
     1981 gcgaccggtc cgatctcggc attcacaggc acggtgactt gcgtgaccag cggctcgatg
     2041 atgcggacga gtgatgagtc gctgacgacg cccacattct gcacgaatgt ctcggtgcgg
     2101 ccgctcatga taatccgttc ggtcgccact tccgtgacgt tccgtatctg cgaggcgggg
     2161 ccggacacga gagcgtgatc gggaacgagc gttgtgtcgc cggcgagata tccgggaggc
     2221 ggttgcccga tcagaaacgg ccggatgggg acgacggtct gccgcagcgc ttccacgtga
     2281 aagcggagtt tcatcggctc gatcgatacg acgtcgatct gcggcgggac gctcatcgcc
     2341 tgcgggctga gcgtaatgac ggcatcgccc ggcgtcaccc aactcacatc gagcgtgact
     2401 tcgagattct gcgatgagag tgatcgaagg tcagagatcc gtccgcggag acgaacgctg
     2461 accgtgtccg gaacaggcgt ggtgatgacg aggtcgcgcg gcatgcggac gatggatacg
     2521 ggcgccgcga acgctcgctc cgagaccggc tcccgccgcg gcgcagagac gaagaaccag
     2581 accgtgaacg caaggaacag ggagaggatg cggagggaga ggttgtggct gctcacgctg
     2641 catctcccgc agcctggcgg ctgccttcgg cgcgttgttg gttgtcggtt ggttgcgcct
     2701 tcggcgcgtt agtgagaacg cgcggcgagg tttttttcaa cgcgccgaag gcgcatctaa
     2761 ccgacaaccg acaacgcgcc gcaggcgcaa t
//
LOCUS       DATLVW010000487         3659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_517642, whole genome shotgun sequence.
ACCESSION   DATLVW010000487 DATLVW010000000
VERSION     DATLVW010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517642"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VNN25_14660"
     CDS             complement(<1..866)
                     /locus_tag="VNN25_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH92822.1"
                     /translation="MKHTDDDLDEEQRRSAEEREPRYCELLVTEHLGDPAEIETRAQI
                     ETSLAAPARRVLVAEDNLLSQQVLARQLAALGFVCDIVTDGGEAFSSVTRGGYDIVLM
                     DLQMPVLDGRSAALLIRRWEKENGAPPVRIVGITASGSAADRRRCLEIGMDDHLQKPL
                     RIADLSSVLNRLLDSSSSTTFVPERNEAGSHPELDHSMIEGLRALGEDDGAFLRGLVR
                     QFRADSDARLARLERAAEDGDTVAIRALAHALCGSSANIGAAGVTAACRAIEDLGATD
                     DVRQYRDAVRRVR"
     gene            1107..1592
                     /locus_tag="VNN25_14665"
     CDS             1107..1592
                     /locus_tag="VNN25_14665"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH92823.1"
                     /translation="MQIHELFAQRWESELPAFGRVLRAMPEEQLDYAPHERSTKAGNL
                     GWQIAEEQRVLCDLLETGVIDWKQRPRPATTAEIVKAWDDATAELRTRIEKLDDAKLA
                     GPANFVMGDGNTWTDTIGNMLWGFLFDMVHHRGQLSAYLRPMGGKVPAIYGPSADDSG
                     S"
     gene            complement(1698..3311)
                     /locus_tag="VNN25_14670"
     CDS             complement(1698..3311)
                     /locus_tag="VNN25_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92824.1"
                     /translation="MATKKKPAKKSASSAGANKALTGKAGKGKDFDPTHESVMALAAT
                     MVEVNAPRPTTTSRYPIPNKTFDALKDKANKVKLAKGSATLAKDSGKKEEVSSMPAMA
                     MAAVPDAPPADAPSPSTNFAGFTATGWIPPDCTMATGPSHVVLSVNSSIAVYPKTGGA
                     PLLQRTLTVWFSNVIQGATIFDPKLLWDQHASRWVLLAVAASTPQQPNLSRYLLSVSA
                     TSNPLGIWRNYSLDAMVDGTTKTNNWADFPALGVDNNALYITSNQFAFGGGFQYAKIR
                     VVPKAGPYSGGTAPFFDFINMKNQNGTTAFTIQPCHTFGAPQVEYLVNSAFPSGNFVT
                     VWRIVSSGTSFTLFRTQVSTSNYALPPNAAQKGNNAPLNTGDVRVLHAVFRGDSIWCA
                     LTTTRNWSGTSSQAAIHWFQIRAAIPALVQEGVYGAANVHYFYPASCPDNNGNMIMVF
                     SRSSSNEYGSCYYTGRKSTDPLGSLQPSALLKAGVANYVGLDGGGRNRWGDYNGVCAD
                     PSNPKGIWFYSLFAGAVNKWATWVGSSFF"
     gene            complement(3333..3557)
                     /locus_tag="VNN25_14675"
     CDS             complement(3333..3557)
                     /locus_tag="VNN25_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92825.1"
                     /translation="MSKPEHGRGSKGQNVNPSELPEELVVLSDEKPRAAPSPGVPVSD
                     EEYKRMKEAAEIAPTRDVKNAQEDRPKKRD"
BASE COUNT          683 a         1095 c         1162 g          719 t
ORIGIN      
        1 cgaacacggc gcacggcatc ccgatactga cggacatcgt ccgtcgcgcc gagatcttcg
       61 atggcacggc aggccgcggt cacgccggca gcaccgatgt ttgcgctgct tccgcaaaga
      121 gcgtgggcga gagcacgaat cgcgacggtg tcgccatcct ctgccgctcg ctccaggcgc
      181 gccaggcggg cgtcgctgtc ggcgcggaat tgtctgacaa ggccgcgaag gaaggcgccg
      241 tcatcttcgc cgagcgcgcg caatccttcg atcatcgaat gatcgagctc cggatggctt
      301 ccggcctcgt tcctctccgg cacaaacgtc gttgacgaag aagaatcaag cagccggttc
      361 agaacggagg agagatcggc gatccgtagc ggcttctgca aatggtcatc cattccaatc
      421 tccagacagc gacggcgatc ggccgccgat ccgctcgccg tgattccgac aatcctgact
      481 ggcggcgcgc cgttctcctt ctcccaccgc cggatcaaga gcgctgcgga gcggccgtcg
      541 agcaccggca tctgaaggtc catcagcacg atgtcgtatc ccccacgcgt cacgctgctg
      601 aacgcctcgc cgccgtccgt aacgatgtcg cagacaaatc ctagggcggc aagctggcgc
      661 gccagaacct gctggctcag aaggttgtct tcggcgacga gaacgcgccg ggctggagcc
      721 gcgaggctgg tctcgatctg cgctcttgtc tcgatctccg ccggatcgcc gaggtgttct
      781 gtgaccaaca gttcgcagta ccgtggctcg cgttcctcgg cggaccgcct ctgctcctca
      841 tcgaggtcgt catccgtatg tttcacgctc gcctcagcgt gaggaatcgg atagccgagg
      901 tgtaaccagc gggtcttctc atctgctgtc gatgagtcgc ctgagcagac gaaaaaatac
      961 agaagcgtct ctacacctgg tggtgtcgcg ggtgacacaa aggggctgcg tgcctaacgc
     1021 ccgagagcgc gtccatcctc ctgcctccac aagccgtaga aatcggaatc agcgtttatg
     1081 ctcatgccga acaaaaggag gcagagatgc agatacacga gcttttcgcg cagagatggg
     1141 aatcagagtt gccggcgttc ggcagagtgt tgcgggcgat gccagaggaa caactcgact
     1201 acgcacccca tgagcgctcc acgaaagcag gcaacctcgg atggcagatc gcggaagagc
     1261 aacgcgttct ctgcgacctg ctcgagaccg gcgtcatcga ctggaagcag cgcccgcgtc
     1321 cagccacgac cgcggagatc gtcaaggcgt gggacgatgc caccgccgag ctgcgcacga
     1381 ggatcgagaa gctcgacgac gccaagctcg ccggccccgc aaacttcgtc atgggcgacg
     1441 gcaacacctg gaccgacacc atcggcaaca tgctctgggg cttcctcttc gacatggtcc
     1501 atcaccgcgg ccaattgagc gcctacctcc gcccgatggg cggcaaggtg cccgcgattt
     1561 acggaccgtc ggcggatgac agcggaagct gaggacgtcg gaggttgaac cctgctttcc
     1621 aacgcggtga caaggattcg ctagcgcgtg ccccagagaa aagcccgctc ctcagtggag
     1681 cgggctctca cgatttctta gaagaacgac gagccgaccc acgtggccca cttgttcacg
     1741 gcgcctgcga acaagctgta gaaccagatg cccttcggat tggaggggtc cgcgcagacg
     1801 ccgttgtagt cgccccatcg gttgcggccg ccgccatcga ggcctacgta gttggccacg
     1861 ccggccttga gtagagcgct cggctgcagc gatccgaggg gatcggtcga cttccggccg
     1921 gtgtagtagc acgaaccgta ttcgttggaa ctggagcgcg agaagaccat gatcatgttg
     1981 ccattgttgt cggggcaact ggccgggtag aagtagtgga cgttcgccgc tccgtagacg
     2041 ccttcctgaa ccagggccgg gatggcggcg cggatctgga accagtgaat cgctgcctga
     2101 ctggaagtgc ccgaccagtt tcgcgtcgtc gtcagcgcgc accagatcga atcccctcgg
     2161 aacacagcat gaagcacgcg aacgtccccg gtgttcaggg gcgcgttgtt gcctttctgc
     2221 gcagcattgg gagggagggc atagttggaa gtggaaacct gcgtgcggaa caaggtgaac
     2281 gaggtgccgc tgctcacgat gcgccacacc gtcacgaagt tgccgctcgg gaatgcggag
     2341 ttcacgaggt actcaacctg cggcgcgccg aacgtgtgac acggctggat cgtgaaggcg
     2401 gtggtgccgt tctggttctt catgttgatg aaatcgaaga acggagcagt cccgccggag
     2461 tacgggccag ccttcggcac gacccgaatc ttcgcgtact ggaagccgcc gccgaacgcg
     2521 aactgattcg aggtgatgta gagcgcgttg ttgtcgacgc cgagggcggg gaagtcggcc
     2581 cagttgttcg ttttggtcgt tccatccacc atcgcgtcga gagaatagtt gcgccagatg
     2641 ccgagagggt tcgacgtcgc cgaaacggac aggagataac gagacagatt cggctgttgg
     2701 ggcgtggaag cggccacagc cagcagaacc caacggctcg cgtgctgatc ccacaggagc
     2761 ttcggatcga agatggtcgc gccctgaatg acgttgctga accacaccgt gagggtgcgc
     2821 tgcagcagcg gggcgccgcc ggtcttggga taaacggcga tggaagagtt cacggagagc
     2881 acgacgtggc ttggaccggt ggccatcgtg cagtcgggcg ggatccagcc cgtagccgtg
     2941 aaaccggcga agttcgtcga tggactcggc gcgtcagcag gcggcgcgtc aggaactgcc
     3001 gccatggcca tcgccggcat cgaggaaact tcttccttct tgccgctgtc tttcgccaga
     3061 gtcgcgcttc ctttggcgag ctttactttg ttcgccttgt ctttcagcgc gtcgaacgtc
     3121 ttgttcggga tcgggtagcg gctggtggta gtggggcgag gtgcattgac ctccaccatc
     3181 gttgccgcca gcgccatgac ggattcgtga gtgggatcaa agtccttccc tttgccagcc
     3241 ttgccggtca gagctttgtt tgcaccggct gatgatgcgc tcttttttgc gggtttcttc
     3301 ttggtcgcca tcttctttcc ccctctcgat agttaatctc tcttctttgg acggtcctcc
     3361 tgtgcgtttt tcacatccct ggtgggcgca atctccgccg cttctttcat tcgcttgtat
     3421 tcttcgtcgg aaaccggcac tcctggtgac ggagcagcac gaggtttctc gtccgagagc
     3481 acaaccagtt cttcgggtaa ctcgctggga ttgacgttct ggcccttgct accgcggcca
     3541 tgttccggtt tgctcactgt cttgccctcc agaaagtgaa gttattgtac gcccagacat
     3601 atctacggga gttgggggtc gggagttggg ggtcgggagt cgggggtcgg gagtcgggg
//
LOCUS       DATLVW010000488         7499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_19909, whole genome shotgun sequence.
ACCESSION   DATLVW010000488 DATLVW010000000
VERSION     DATLVW010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19909"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VNN25_14680"
     CDS             complement(<1..378)
                     /locus_tag="VNN25_14680"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HXH92826.1"
                     /translation="MKPTAIAAVILLAATTTFAAEVVKRGAPLSSSKPVALETVLESP
                     KAFTKDAVVVEGVIQKSCTNMGCWMELAPAPGKKSVRVTFKDEKFSIPLTAKGMQARA
                     EGVTTIKTLSKAEADHLAEEGATL"
     gene            complement(446..2656)
                     /locus_tag="VNN25_14685"
     CDS             complement(446..2656)
                     /locus_tag="VNN25_14685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH92827.1"
                     /translation="MTLPGGTRLGPYEIGELIGRGGMGEVYRAADTRLGRDVAIKVLA
                     THLADDPASLARFQREARAVAALSHPNIVALFDVGSEGQTHYVVTELLEGETLRGRLS
                     ASRLPEDEILRIANAIADGLAAAHAKGIIHRDLKPENVFLTSAGGVKILDFGLASMQQ
                     TYAGIDTAIQTAALTEPGVLMGTIGYTSPEQLAGRPLTTATDVFSFGCVLYEMLQGQR
                     PFKRDSNMEIIASVLRDAPFDRDAAKHLPAGMRDIVEQCLEKDPRERFPNGAAVAAAL
                     RNRHSVVSSRIEPKKSPRTLTWIAGSVVIVIAAAIVAFWLIHRQAEVIDDGYDLRSAD
                     VTGDGETRRLLALALHADSAGDRSEAMQLLAEAARRNGAPPLPAAFLASFTYYSGDRK
                     EGSRWSAEATRRIASSTSTYEALLCRYLLPGNSTGTEMALSSSLLELRPKAWRLRLAL
                     AHLHLARRELPAALAQLQRIDVGAPDDRRLAIVLADRASLGDVAGATRDLQRSKLAGK
                     PALLAYTRGRIAWSSGQASEAARLFDAAAESATISNLGPVAIESRILAGVARIGANDL
                     GGAASAFDLAAVKAHDAAMPENELEAYAFGAYVASRRGDTDGRDRRMRLAAKLAEPGT
                     ANYLALRLFAARENVSTAFGAAPVPNEADENSAVATLIKARDAWSQKDPAAASGLLEQ
                     ARSEGIEATWFSEEAALLAYDLGAPPRTFPPDPPYPNRLRFIAVWELAGPRASR"
     gene            complement(3051..3503)
                     /locus_tag="VNN25_14690"
     CDS             complement(3051..3503)
                     /locus_tag="VNN25_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HXH92828.1"
                     /translation="MKTRNKILLGLLAAFVFIQLIPVPKTNPPVTGEIAAPPEVKSVL
                     RKSCYDCHSNETVWPWYSRLAPVSWLLYRDVTQGRRTMNFSAWQHMPQDRQNRRRKGV
                     WDEVASGDMPLWFYKPLHPDAKLTDADKATLKAWSDAGPAVPKRNAGG"
     gene            complement(3636..4304)
                     /locus_tag="VNN25_14695"
     CDS             complement(3636..4304)
                     /locus_tag="VNN25_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92829.1"
                     /translation="MKRLLLLAATVALAACSTTTKHHDSNPYANPFYAKYLNTGAWVD
                     NEITRTINDLRANPRSATLHNTLGQLLIQKGFPKDAETEFERAVNADSHFYPGWYNLG
                     LVRMSRGDLIGAHHAFSRTIHYKPGHSAALFQLGLMEEKSNHQSAAIDYYAKAFTINP
                     ALLDVRINPRILDCNLVDLALLKAYPNDHARQSMAFNPAPPGYVQEGLSTPAAVPQLV
                     KPPK"
     gene            complement(4307..4897)
                     /locus_tag="VNN25_14700"
     CDS             complement(4307..4897)
                     /locus_tag="VNN25_14700"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HXH92830.1"
                     /translation="MIKQDDELDKLEEDIRKIKNKYDQFFSGIQKLPPMHERRLLEVY
                     IFELGKQKMRDNTRRFRYSQLLTRYNQLRELWGRKMREREEGPIDFRRRAAALREPAP
                     PPPPPAPRVTSARADSYVTVAPGSNGEEIQRLFGEIEKEQLKLGRLPNITIEQLKTMV
                     QKQSELVRAKYHVAAVAFRVDVVDGKVKLKAKPIQE"
     gene            5026..6375
                     /gene="selA"
                     /locus_tag="VNN25_14705"
     CDS             5026..6375
                     /gene="selA"
                     /locus_tag="VNN25_14705"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXH92831.1"
                     /translation="MPKATADSGDSSLRRIPSVERILSSESLRDMVAQFGRERVKDAV
                     CLHLETIRRDRVLFEESAAASDVRARLDVATESTLKPVINGTGIIIHTNLGRSPIDPA
                     LWSRAAAVTTNYSNLEFDLEEGSRGRRDEHLTELCRTLFGCESAVLANNNAAGTLLLL
                     AAVAAGKEVIVSRGELVEIGGSFRVPDVIQQGGARLREVGTTNRTHAADFDEAASDAS
                     AAILRVHRSNFEIVGFTETPSIDQLVAVARDHRLPLLYDEGSGRVVDLSPYGFSRQPT
                     VRELFASGVDAITCSTDKLIGATQGGLILGREDIIRRCRKHPLMRALRAGKESYAVIA
                     ETLRAFATARYETEIPIYRMLATPLDTLRAHAEEMVRGTKSRVIESQCALGGGTTPTE
                     TIRSIAIEVGGDASALAARFLRYSTPIAGRIVDDRFTIEVRTLGDREREIVAEAIHG"
     gene            complement(6641..7390)
                     /locus_tag="VNN25_14710"
     CDS             complement(6641..7390)
                     /locus_tag="VNN25_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXH92832.1"
                     /translation="MVRTIWAVIVAVAVTIPLAMAVLIIAMLSSASPLIEPIIRWWAR
                     AIVWGAGIDLRSDGTERIDRGKRYILIANHHSYFDIPCIFAAIPQPIRFMAKKSLFSI
                     PIFGWAIGRAGFIPIDRKNRRTAVKSFDLAVERIRRGNTIVIFPEEGRSREVAMRPFQ
                     RGAFLLAIRSELPILPLAIDGTYEVFRVGAKTITPGVVTVRVGTEIETAGAGVRAKDA
                     LSEQSRSQIEMMLAGSSVSTDSKSVAAARLH"
BASE COUNT         1307 a         2283 c         2446 g         1463 t
ORIGIN      
        1 cagcgttgcg ccctcttcgg cgaggtgatc tgcctcagcc ttgctcagcg tcttgatcgt
       61 cgtcacgcct tcggcacggg cctgcattcc tttggcggtg aggggaatgc tgaacttctc
      121 atccttgaac gtgacgcgca cactcttctt gcccggagcc ggcgcgagct ccatccagca
      181 acccatgttg gtgcacgact tctggatcac gccttcgacg acgaccgcat ccttcgtgaa
      241 cgccttcggc gattcgagaa ctgtttctaa tgcgaccggc ttggaggagg ataacggtgc
      301 tccgcgcttg acgacttcgg cggcgaaggt cgtagtggcg gcaagcagga tcacggcggc
      361 gattgctgtg ggtttcatgg tctgcgaagg atatcgcggc gccgattgag tcgctgtaaa
      421 caaggattaa caaagaaacg aacaatcagc gcgaagcgcg cggaccagca agttcccaca
      481 ccgcgatgaa ccgcaagcga ttcgggtagg gcgggtcggg cgggaatgtg cgtggtggcg
      541 cgccgaggtc gtaggcgagg agggcggctt cttcgctgaa ccaggttgct tcgataccct
      601 cggaacgcgc ctgctccagc agccccgatg ccgctgctgg atccttttgc gaccatgcat
      661 cgcgagcttt gatcagcgtc gcaaccgccg aattctcgtc ggcctcgttc ggtactgggg
      721 cggcgccaaa ggcagtgctg acgttttcgc gcgctgcgaa gagacgcaac gccagatagt
      781 tcgccgttcc cggctcggcg agttttgcgg cgagtcgcat tcggcgatcg cggccgtccg
      841 tgtcaccgcg gcgcgatgcc acgtatgcgc cgaatgcata ggcttcgagc tcattctccg
      901 gcatcgctgc gtcgtgcgcc ttcactgcgg cgagatcgaa cgcggacgct gcgccaccga
      961 ggtcgttcgc gccgatgcgc gcaacgccgg cgaggatgcg cgattcgatc gcgaccggcc
     1021 cgaggttgct gatcgtggcg ctctccgcgg cagcatcgaa caatcgcgct gcttcggatg
     1081 cttgtccact gctccaggca atacggccgc gcgtgtaggc gagcaacgcc ggcttgccgg
     1141 ccagtttgct tcgctgcaga tcgcgggtgg ctccggccac atcgcccagc gaggcgcggt
     1201 cggcgagcac gatcgcgagg cggcggtcgt cgggggcgcc gacatcgatc cgttgcagtt
     1261 gcgcgagtgc ggccggcagc tcgcgccggg cgagatgcaa atgcgcgagc gccaggcgca
     1321 ggcgccacgc tttcgggcgc aactccagaa gcgacgatga cagcgccatc tccgtgcccg
     1381 tgctgtttcc cggcagtaga tagcggcaga gaagcgcctc gtaagttgat gttgagctgg
     1441 cgatgcgcct cgtcgcttcg gcggaccagc gcgagccttc ctttcggtcg ccgctgtagt
     1501 acgtgaacga agcgaggaac gcggcgggaa gcggcggcgc tccattgcgc cgcgctgctt
     1561 cggccagcag ttgcatcgcc tcggatcggt cacccgcgct gtccgcatgc agcgcgagag
     1621 cgagcagacg gcgcgtttcg ccgtctccgg tgacgtcggc gcttcgcaag tcgtagccgt
     1681 cgtcgatgac ctcggcctga cgatggatca accagaatgc gacgatggcg gccgcaatca
     1741 cgatgaccac agatccggcg atccacgtca gtgttcgcgg cgactttttc ggttcgatgc
     1801 ggctgctgac aaccgaatga cgattgcgaa gggctgcggc gacggcagcg ccattcggga
     1861 aacgctcgcg tggatctttc tccagacatt gttcgacgat gtcccgcatg ccggccggaa
     1921 gatgcttcgc tgcgtcgcga tcgaaaggag catcgcgaag caccgaggcg atgatctcca
     1981 tgttgctgtc ccgtttgaac ggacgctgcc cctgcaacat ctcgtacaga acgcagccaa
     2041 acgaaaagac gtcggttgct gtcgtgagcg gcctgccggc aagctgctcc ggcgatgtgt
     2101 agccgatcgt tcccatcaga acaccgggct cggtcaacgc ggctgtctgg atcgcggtgt
     2161 cgatccccgc gtaggtctgc tgcatgctgg ccagaccgaa gtcgaggatc ttcacgcccc
     2221 cagcgctcgt cagaaagacg ttttccggct tgaggtcccg gtggatgatg ccttttgcgt
     2281 gcgcggctgc gaggccatcg gcgatggcgt tcgcgatccg cagaatctcg tcttccggca
     2341 gccgcgatgc ggacagacgc ccgcgaagcg tctctccttc cagcaactcc gtgacgacgt
     2401 agtgtgtctg cccttcgctg ccgacatcga acaaggcgac gatattcgga tgcgagagtg
     2461 ccgcgaccgc gcgggcttcg cgttgaaaac gtgcgagcga ggccggatcg tcagcgaggt
     2521 gcgtggcgag aactttgatg gcgacatcgc ggccgaggcg cgtatcggcg gcgcggtaga
     2581 cctcgcccat gcctccgcgg ccgatcagct ctccgatctc gtagggtccg agccgcgtac
     2641 cgcccggaag tgtcatgcaa ggagtatgga cgagatcggg gatcggagga tgggacgatc
     2701 agaatcgtgc agtcaatttt cggcgacggc tggagggttc ttgccaagcg agaaacgctc
     2761 gacaacctcg agcgccgacc aaacatcgag cgccttctcc cccacgcttt tcagtggggg
     2821 agaaggtgcc gaaggcggat gagggggcgt tggaaatgat ccgtggacgg gcaacaatcc
     2881 gacaagcatg ctcacaaact gcctggcatt ggccgcagtt cgcgcccatt gcaatccccc
     2941 ctcatccggc ttcgccacct tctcccccgc aaaaagcgcg ggggagaagg cgctcgattg
     3001 gtaggttggg gctttggggg caggacagag ctgagaatcg gccgctcgcg tcatcctcct
     3061 gcattgcgtt tgggaactgc cggtccagca tcgctccacg ctttgagtgt ggctttgtcg
     3121 gcgtcggtga gctttgcgtc cgggtggagc ggcttgtaaa accagagcgg catgtcgccc
     3181 gatgcgactt cgtcccagac tcccttgcgg cgcctgttct gccgatcctg cggcatgtgc
     3241 tgccaggcgc tgaagttcat cgtccggcgt ccctgcgtca cgtcgcggta gagcagccac
     3301 gaaacgggcg cgaggcggct ataccagggc cagacggtct cgttggaatg gcagtcatag
     3361 cagctcttcc gcagcaccga tttcacttcc ggcggcgccg cgatctcgcc ggtcacaggg
     3421 gggttggttt tcggaaccgg aatgagctga atgaatacga atgccgccag gagaccgagg
     3481 aggatcttgt tccgcgtctt catgcgggaa gagtaatgcc tctcgcgtgc cgatgactca
     3541 gagatcagag acagttagag atcagagatc agagatagcc gatgaggcgg cagacagccc
     3601 ctgatctctt gtctctgatc tctgtctcgg atcttctact tcggaggctt cactagctgc
     3661 ggcacggcag ccggcgtgct gaggccctcc tgcacatagc ctggcggtgc gggattgaag
     3721 gccatgcttt ggcgagcatg atcgttcgga taggccttga gcagggcgag gtcgactagg
     3781 ttgcagtcga ggatgcgggg gttgatgcgc acgtccaaca gagcgggatt gatcgtgaac
     3841 gcttttgcgt aatagtcgat cgcggcgctt tgatggttgc ttttctcttc catcaggccc
     3901 agctggaaaa gggctgctga atggccgggc ttgtaatgga tggtccggga gaaggcgtgg
     3961 tgggcaccga tgagatcacc gcgggacatg cggacgagcc cgaggttgta ccagccggga
     4021 tagaaatgcg agtcggcgtt gacggcgcgc tcgaactcgg tctccgcgtc cttcggaaaa
     4081 cccttctgga tcaggagctg cccgagggtg ttatgcagtg tggccgagcg cggattggcc
     4141 cgcaggtcgt tgatcgtgcg cgtgatctcg ttgtcgaccc atgccccggt gttgaggtat
     4201 ttcgcgtaga acgggtttgc gtacggattc gagtcgtgat gcttcgtggt ggttgaacag
     4261 gcggccaaag ctacggttgc ggcaagaagt aggagtcgtt tcatcatcat tcctggattg
     4321 gtttcgcttt cagtttgact ttgccgtcga cgacgtccac acgaaaggcg acggcggcga
     4381 cgtgatattt cgcacgaacg agttcgcttt gcttctgaac catggttttc agttgctcga
     4441 tggtgatgtt cggtaaccgt ccgagtttga gttgctcttt ctcgatttcg ccgaagagcc
     4501 gctggatctc ctcgccgttc gagcccggcg ctactgttac atacgaatca gcccttgccg
     4561 acgttacacg aggggctggc gggggtggcg gcggggccgg ttccctcagg gcggcggcac
     4621 ggcgccggaa gtcgatcggc ccttcttccc gctcgcgcat cttccggccc cacaactcgc
     4681 gcaactggtt gtaacgggtc agcagttgcg agtagcggaa gcggcgcgtg ttgtcgcgca
     4741 tcttctgttt tcccagctcg aagatgtaca cctcgagcag ccggcgctcg tgcatcggcg
     4801 ggagcttctg gataccggaa aagaactggt cgtacttgtt cttgatcttc cggatgtcct
     4861 cttcgagctt gtcgagctcg tcgtcctgtt tgatcattcg cgggagtcgg ttctcgggag
     4921 ttgggtgtcg ggggtcgggt gtcggagaaa agcgaaaaac ctcgcctgcc tgtctccgac
     4981 tcccgacccc cgacacccaa ctcccgcaac tacaatacgc gctccatgcc caaagcgacg
     5041 gctgattcag gagattcctc actgcggcgc atcccgtccg tcgagcggat tctctccagc
     5101 gagtcgttgc gggacatggt tgcgcaattc ggccgtgagc gggtgaagga tgccgtctgt
     5161 ctccacctgg agacgattcg ccgggaccgc gtcctgttcg aggagagcgc agccgccagc
     5221 gacgttcggg cacgccttga cgtcgccacg gaatcgacgc tgaagccggt gatcaatggc
     5281 accggcatca tcatccacac gaaccttggt cgctcgccga tcgatcccgc gctctggtcg
     5341 cgtgccgccg cggtcaccac caactactcg aatctcgagt tcgacctcga agaaggctcc
     5401 cgaggccggc gcgacgagca tttgacggag ctgtgccgca cgctgttcgg ctgcgaatcg
     5461 gcggtgctgg cgaacaacaa cgccgccggc acgctgctcc tgctcgcggc cgtcgccgcc
     5521 ggaaaggaag tgatcgtttc gcgcggcgaa ctggtcgaga tcggcggttc cttccgtgtc
     5581 cccgacgtca ttcagcaggg tggcgcacgg ttgcgggaag tgggaacgac caaccgtacg
     5641 cacgccgccg acttcgacga agcggcctcc gacgcctcgg cagcgatcct tcgggtccat
     5701 cgatcgaatt tcgagatcgt cggtttcacg gagacgccgt cgatcgatca gctcgtggcc
     5761 gtcgcgcgcg accataggct gccgcttctt tatgatgaag ggagcgggcg cgtcgtcgat
     5821 ctctcgcctt acgggttctc gcggcagccg accgttcgtg agcttttcgc cagcggcgtc
     5881 gacgccatca cgtgctcgac cgacaaactg atcggtgcca cgcagggcgg tctcatcctg
     5941 gggcgcgaag acatcatccg gcgctgccgc aagcatcccc tcatgcgcgc gctgcgggcg
     6001 gggaaggaat cgtacgccgt catcgccgag accttgcgcg cgttcgcgac ggcccggtac
     6061 gaaacggaga ttccgatcta ccggatgctg gcgacgccac tcgacacgct gcgagcgcat
     6121 gccgaggaga tggtgcgggg gacgaagagc cgggtgatcg agagccaatg tgcgctggga
     6181 ggcggcacca cgccgaccga gacgatccgc tcgatcgcga tcgaagtggg cggcgatgca
     6241 agcgcgctcg cagcacgatt cctccggtac tccaccccga tcgcaggccg catcgtcgac
     6301 gaccgcttca cgatcgaagt gcggacgctt ggagatcggg agcgcgagat cgtggccgaa
     6361 gccatccacg gataacggac tcgcccgctt aactttgtca gtttgggcgt gcactccgta
     6421 actgcacctt gctgcaaatg atcgacaggt gtaattttat cgagatcgca gcaacggatc
     6481 ggagccgtga tcacgctcct ccctggcatc gatggccggg ctggccggtg ccgaaggatt
     6541 ttccggccgc gcgacgcatc attcgtcgcg cggccgcggg ctggttgctt tcgccctcgt
     6601 tgacgcgatc aagcgggtca accgcgtctt cagtctgccg tcaatgaagt cgtgctgctg
     6661 cgacgctctt cgagtccgtg ctgacgctgc tgcccgcgag catcatctcg atctgcgacc
     6721 gcgattgctc gctgagcgcg tccttggcac ggacgccggc acccgcggtc tcgatctcgg
     6781 tgcccacacg caccgtgacg actcccggtg tgatcgtctt cgcgccaacg cggaacactt
     6841 cgtaagtgcc gtcgatcgca agtggcagga tcggcagctc ggagcggatc gccagcagga
     6901 acgcaccgcg ctgaaacggt ctcatcgcca cctcgcgcga gcgcccttct tccgggaaga
     6961 tgacgatcgt gttgccgcgc cggatccgct cgacggccag gtcgaatgac ttgaccgcgg
     7021 tgcgccggtt ctttcgatcg atcgggatga agccggcgcg gccgatggcc cacccgaaga
     7081 tcggaatgct gaacagactc ttcttggcca tgaaccggat cggctgcggg atcgccgcga
     7141 agatgcatgg gatatcgaag tacgagtgat ggttggcgat gaggatgtag cgcttaccgc
     7201 ggtcgatgcg ctcggttccg tcgctgcgca gatcgattcc cgctccccag acgatcgcgc
     7261 gcgcccacca acggatgatg ggctcgatca acggcgacgc cgacgacagc atggcaatga
     7321 tcagcaccgc catggccagc ggaatcgtca cagcgacggc aacgatcaca gcccatatcg
     7381 ttcgaaccat tcgccgcgca tcttatgacg gagtgcggtc gtctcgaccg cgggcactcg
     7441 ggcgtccccg cccgagtgct gtcagggcgg aggccgagat tcgtcgaggc tctcgttgg
//
LOCUS       DATLVW010000489         6483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_189652, whole genome shotgun sequence.
ACCESSION   DATLVW010000489 DATLVW010000000
VERSION     DATLVW010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189652"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..186
                     /locus_tag="VNN25_14715"
     CDS             10..186
                     /locus_tag="VNN25_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92833.1"
                     /translation="MTRRGEEMAVLVRVDHWRQLEEATRPRLKDLLLAAAPRAEVPVT
                     PRRARRRREPPAFE"
     gene            193..600
                     /locus_tag="VNN25_14720"
     CDS             193..600
                     /locus_tag="VNN25_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH92834.1"
                     /translation="MYLLDTNVVSELRKPRPHGAVLAWLKGTADENLHLSAVTLGELQ
                     AGVEITRERDQEKASEIEVWIDQVAQTWNMLPADARVFRVCAKLMHRRPDHLFEDALI
                     AATAIVHHLVVVTRNVRHFTVFQVDTLDPFATK"
     gene            complement(677..1225)
                     /locus_tag="VNN25_14725"
     CDS             complement(677..1225)
                     /locus_tag="VNN25_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92835.1"
                     /translation="MSDNVNNTPGTSNSDPANLSAEEVIAQLRTMRSRIGDVSPLSKD
                     RRKLVKMRLRTQTAPVVEASISVIGSSDKVAHAVGQPLEEVLQLQNDSVHWSLVADEL
                     RSFLKGIEGANLLRREELAFIASQAYSFGSQLARSPVNADLVPQVEEIKRLKTLARRK
                     KASQNPPPPSPAHETSTTPNVD"
     gene            1875..2012
                     /locus_tag="VNN25_14730"
     CDS             1875..2012
                     /locus_tag="VNN25_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92836.1"
                     /translation="MADSEYTVGAAESRRPCLLPAGVLARRGFSKWGYNSTRVPLQAR
                     R"
     gene            complement(2111..2185)
                     /locus_tag="VNN25_14735"
     tRNA            complement(2111..2185)
                     /locus_tag="VNN25_14735"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2151..2153),aa:Gln,seq:ttg)
     gene            complement(2296..3261)
                     /locus_tag="VNN25_14740"
     CDS             complement(2296..3261)
                     /locus_tag="VNN25_14740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92837.1"
                     /translation="MPTKVIKEIAALTALEAEWLDLCHRTPDATPFQTPMWLLPWWRA
                     FGSDDLAVIAARDEADLRSLAPLYIVRDEDESLGLFIGTGISDYLDILGDTAPIMPEL
                     AGIDCQLWDLQQLRPSSTLLQSPLPEGWSENIEDQEPCPTLSIDGAGPELENLISTHF
                     RKKIRYYRRALSRMGDVAVETPTTDTLDDLLTAFFDLHTARWKRRNLPGVLADETAQR
                     FHREVARRMLAQGMLRMYATRVAGRIVAVFYGFALHGTVYYYLSGYDPDLEKLSIGTL
                     IVAHAIEEAVREGATTFDFLRGAEEYKYAWGATDRMNRRRQLTRG"
     gene            3321..4178
                     /locus_tag="VNN25_14745"
     CDS             3321..4178
                     /locus_tag="VNN25_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH92838.1"
                     /translation="MSDKDFVTIDTTHLHIEGRSRAGHETWFRVRELNAAFDIGRCPD
                     TLIATPHIFVTHAHLDHAVGVPFYAGQRQLHGMTGGHVYVPADAADGMREIMAIYERI
                     TGASFAEIEVIGLAPGDVVRIGKTHEVRTHRATHRVAANAYEVIEVRHRLKDEFASRD
                     ERDIIDLRHLGTQVTEVWRRPMFFYTGDTDRRILEENEALFKAEVLMIECSFIVAGHE
                     ERAAKYRHIHFDDIAAFAGRFENETIVLTHFSRRYSRSQIHDEIRRRCPAVLRERIRL
                     ALPDPYQRL"
     gene            4276..5799
                     /gene="dacB"
                     /locus_tag="VNN25_14750"
     CDS             4276..5799
                     /gene="dacB"
                     /locus_tag="VNN25_14750"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HXH92839.1"
                     /translation="MTVRRTRFIATALLLLFFQVLTAAGADTAKRRRGKSRRRKAAPA
                     APADVATGSTLSERLTSLMSGSVARSSDTSLQVVDVESGNLIAERGPRTPLAPASNMK
                     LFTTAAAIDLLHPDFEVTTTVFMRGTVDAGGTLTGDVKVVGRGDPTIGGRFHDGHATA
                     VIDEWATDLKRAGVKTVHGNLIFEYGYMDTQYIHPTWPVNQLVNWYEAPVSAFSMQEN
                     CVQVRVSPTKPGKACLVQFEPPTAYLDLETSCVTGRGLPFITRYRNTNKILVHGGTPA
                     RAGVTEVFVTVENPVQYFATVTAETFVRDGVAVQGQVVVTPHDDRPDWKAVSQHATPL
                     NVVVYVINKKSQNHYAEELVKIIGAETKKQGTWAAGTGSVAEWLTGKLTVPAEEFHQA
                     DGSGMSRENRASANAFIHLLRYMWKSPYREDFVSSLPYSGDPDAKFGHRLRGAPYARQ
                     VYAKTGYIVGVVGLSGYVRGASGKVYAFSFVFNNYHVGVYAVYQLQDSMLKEIITRG"
     gene            5972..>6483
                     /locus_tag="VNN25_14755"
     CDS             5972..>6483
                     /locus_tag="VNN25_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92840.1"
                     /translation="MKKETIIFTTCGFLLGLTLGGLVIGPKIAQSKLAGAAPVSETES
                     AAPAATPPMGAAGGNPMAAVQQQLATLKATLEKDPKNFDALVQLGDMYMDAAKFPQAI
                     DYLDRALAVHDDANVRTDLGICYKQTGQPASALAEFERAASMAPDQWQPLFNQAIVLN
                     DLHRTDEARVV"
BASE COUNT         1219 a         1954 c         2104 g         1206 t
ORIGIN      
        1 ccgcagattg tgactcggcg cggagaggag atggctgttc ttgtacgcgt cgatcactgg
       61 cgccaactgg aagaggctac acgcccgcgg ctgaaggact tgttactggc cgccgcaccg
      121 cgagcggagg ttcctgtgac cccgcgccgg gcacggcgcc gccgggagcc acctgcgttc
      181 gagtgagcga tcttgtactt actcgatacg aatgttgtgt ccgaattgcg caagccgcgg
      241 cctcacggcg ccgtgctggc gtggctcaag ggaaccgcgg acgagaacct tcacctctct
      301 gcggtcacgt taggcgagtt gcaggccggt gtcgaaatca ctcgtgaacg cgaccaggag
      361 aaggcttccg aaatcgaggt ctggatcgat caggtggcgc agacctggaa catgcttccg
      421 gccgatgccc gcgtcttccg ggtctgcgca aaactcatgc accggcggcc tgaccatcta
      481 ttcgaggatg cgctgattgc tgccactgcg atcgtgcacc atctggtggt tgtgacgcgc
      541 aacgttcgtc acttcactgt cttccaggtt gacaccctcg acccgttcgc aacgaagtga
      601 gcggcgagaa gcgcttggat accactcata tggaacccgg gcgcgcctgc gagcgcgccc
      661 gggttcacag cctcctctaa tctacgttcg gtgtcgttga cgtctcgtgt gcaggagacg
      721 gcggcggcgg attctgcgac gccttcttgc ggcgcgcgag agtcttcagg cgcttgatct
      781 cctccacctg cggcaccaga tcggcgttga cagggctccg cgcgagctgc gatccgaacg
      841 agtaggcctg cgatgcgatg aacgcgagct cctcgcgacg gagcagattc gcaccttcga
      901 tgcccttgag gaacgagcgg agctcatcgg cgacgaggct ccagtggaca gagtcgttct
      961 gaagctggag aacttcctcg agcggctggc ctacagcgtg tgccaccttg tccgacgagc
     1021 cgatcacgct gatcgacgct tcgacgaccg gcgctgtctg cgtccggaga cgcattttga
     1081 ccagcttgcg ccgatccttc gacaacggcg agacgtcacc gatccgcgac cgcatggtgc
     1141 gtaactgcgc gatcacctct tcggcactca ggtttgccgg atccgagttt gatgtaccag
     1201 gggtgttatt gacgttatca ctcatgacat accttcctta tgcgagctaa ggccggaggt
     1261 gacgtcacga ctcgtcgaca agtgcgagcg gccaccggct ggaagtcccg gccaagcgcc
     1321 gattcttccg gttgatgttt gcccgaggta tgtacagacc tcgggtgccc gcggcaggag
     1381 gagatcaact caactcccgc gccggtacag gacacatcat gctatccagc acgcccgtac
     1441 cgccgacagc cgccaaagcc ggaggtctta cgatcttcga ctttgtagtg aacgatatag
     1501 gcccgtggaa acgggttgtc aagggcaatg cgaggaaact acggagtgga acactgcaac
     1561 gcgatgcgtg cgcacctgcg caccgcgcgt gcgacgatgc ggcatggccg gtgcgcatgt
     1621 gtgcaccgag ggtgcaacgc tgtgacacgg tgggtgtgca cgcgtgcaat ggtgtgcaac
     1681 gttgtgacac ggtggtgcgc acgtatgcat cgacggtgca acgttgcggc acggtgtgtg
     1741 cgcatatgtg caccgagggt gtgtggggct gtgacacggt agatgcgcat gtgtgcaccg
     1801 agagtgcaac gttgtgacac ggtggtgcgc acgtgtgcat cgaaggtgaa acgttgcgac
     1861 acggcggata gatgatggcc gacagcgagt acacggtcgg cgcggcggag agcaggcgtc
     1921 cctgcctgct accggcgggc gtcctcgccc ggcgggggtt ttcgaaatgg ggctacaact
     1981 caacacgcgt tccgttgcag gccaggcgct gaaaaaacac cgccgggcag ggacgcccgt
     2041 cggatgcagg cagggacgcc tgcactacgt cgcgtcgttg atgccgttgc gggccgtcga
     2101 ctacttggat tggctgggga gggaggattc gaacctccga atggcggctc caaaggccgc
     2161 tgccttaccg cttggcgact ccccagtgtg gtggacgcga aactcctaaa accaaatggt
     2221 atcgcggtca ttcctgatgt cttcgtctcc gcgccctccg cgtctccgcg tgaaacggtt
     2281 ttcggtgaga agcgctcagc ccctggtcaa ctgccgccgg cgattcatcc gatccgttgc
     2341 cccccacgcg tacttgtact cctcggcgcc gcggaggaaa tcgaacgtcg ttgcgccctc
     2401 gcgaaccgct tcttcgattg cgtgggcgac gatcagcgtt ccgatgctca gtttttccag
     2461 gtcgggatca tagccgctca ggtagtagta gaccgtgcca tgtagcgcga agccgtagaa
     2521 gacggccacg atgcggccgg cgacgcgcgt ggcgtacatc cgcagcatgc cctgagcgag
     2581 catccggcgc gccacctcgc gatgaaaacg ctgcgcggtt tcgtcggcga gaacgcctgg
     2641 aaggttgcgg cgtttccagc gcgcggtgtg gagatcgaag aacgcggtga ggaggtcgtc
     2701 gagcgtgtcg gtagtcggcg tttctacagc cacgtcaccc atgcgggaaa gcgcgcggcg
     2761 gtagtagcgg atcttctttc gaaagtgggt ggagatgaga ttctccagct ctggtccagc
     2821 gccgtcgatc gagagagtcg gacaaggctc ctgatcttcg atgttctccg accagccttc
     2881 cggaaggggc gactggagca gcgttgacga tggccggagt tgctggaggt cccagagctg
     2941 gcaatcgatg ccggccagtt cgggcatgat cggcgcggtg tcgccgagga tgtcgaggta
     3001 gtcgctgatg ccggtgccga tgaaaaggcc gagcgactcg tcttcgtcgc ggacgatata
     3061 aagcggcgcc agcgaccgca ggtcagcttc gtcgcgggca gcgatgacgg cgagatcgtc
     3121 ggagccgaag gcccgccacc acggaaggag ccacatcggc gtctggaatg gagtcgcgtc
     3181 cggcgtgcgg tggcagagat cgagccactc cgcctcgagt gcggtgagcg ccgcgatctc
     3241 cttgatgact ttggtcggca tgagcggcgg attgtaaccg ccggatttcg cggcgcaatg
     3301 tgtcatcctc atgctgaggc atgagcgaca aagacttcgt cacgatcgac acgacgcacc
     3361 tgcacatcga gggacgttcc cgcgcggggc atgagacctg gtttcgcgtt cgcgagctga
     3421 atgcggcgtt cgacatcggc cgctgccccg acacgctcat cgctacgccg cacattttcg
     3481 tcacgcacgc gcatctcgac cacgccgtcg gcgtcccctt ctatgcgggg cagcggcagt
     3541 tgcacggcat gaccggcgga catgtctacg ttccggccga tgccgcggac ggcatgcggg
     3601 agattatggc gatctacgag cgcatcaccg gcgcgtcgtt tgcggagatc gaggtgatcg
     3661 gtcttgcgcc cggcgacgtc gtgcggatcg gaaagacgca cgaggtgcga acgcatcgcg
     3721 ccacgcaccg tgttgccgcg aacgcgtacg aggtcatcga agtgcggcat cggctgaagg
     3781 atgaattcgc gtcgcgcgac gagcgggaca tcatcgacct gcgtcacctc ggcactcagg
     3841 tcacggaggt gtggcgccgg ccgatgttct tctacacggg cgacacggac cggcgcatcc
     3901 tcgaagagaa cgaggcgctc ttcaaagccg aggtgctgat gatcgagtgc tcgttcatag
     3961 tggcggggca tgaagagcgt gcggcgaagt accggcacat tcatttcgat gacatcgccg
     4021 ccttcgccgg acgtttcgaa aacgaaacga tcgtgctgac gcacttcagc aggcgctatt
     4081 cacggagtca gatccatgat gagattcgac gccgatgccc ggcggttctt cgagagcgca
     4141 tccggctggc gttgccggat ccgtatcagc ggttgtaagt ctgcgatggt gccgatggcg
     4201 cgcccgcacg gcgtgcaccg aagctcgcac gtgccttgca gaattgccca ggggttggaa
     4261 aggttcctcg tactaatgac ggttcgacgc acgcgtttca tcgcgacagc gcttctcctt
     4321 ttattcttcc aggttctcac ggcagccggg gctgacaccg caaagcggcg tcgcgggaaa
     4381 tcgcgccggc ggaaggctgc acccgccgca cctgcggacg tggccaccgg ttcgacgctg
     4441 tccgaacggc tcacgtccct gatgtccggt tcggtggcgc gcagctcgga cacgagcctg
     4501 caggtcgtcg atgtcgagag cggcaacctc atcgccgagc gcggtccccg cacgccgctg
     4561 gcgccggctt cgaacatgaa gcttttcacg accgctgcgg cgatcgatct gctccatccc
     4621 gacttcgaag tcacgacgac cgttttcatg cgcggtaccg tcgatgccgg cggcacgttg
     4681 accggcgacg tcaaagtggt ggggcgaggc gaccccacga tcggcgggcg ctttcacgac
     4741 ggtcacgcca ccgcggtcat cgacgaatgg gctaccgatc tgaagcgggc gggcgtcaag
     4801 acggtgcacg gcaacctcat tttcgagtat ggctacatgg acacgcagta cattcatccg
     4861 acctggccgg tcaatcaact ggtgaactgg tacgaagcgc cggtctccgc attctcgatg
     4921 caggaaaatt gtgtgcaggt gcgggtatcg ccaacgaagc cggggaaggc gtgtctcgtg
     4981 cagttcgaac cgccgacggc atatctcgat ctggagacgt cgtgcgtaac cggccgcggc
     5041 ctgccgttca tcacgcgcta ccgcaacacg aacaagatcc tcgtgcacgg aggaacgcct
     5101 gcgcgagccg gcgtcaccga ggtcttcgtc acagtcgaga atccggtgca gtacttcgcc
     5161 accgtcaccg ccgagacctt cgtgcgcgac ggcgtggcgg tgcagggaca ggtcgtcgtc
     5221 acgccgcatg acgaccggcc ggattggaag gccgtatcgc aacacgccac gccgctcaac
     5281 gtcgtcgtct acgtcatcaa caagaagagc cagaatcatt acgccgagga gctggtgaag
     5341 atcatcgggg cggagacgaa aaaacagggg acgtgggcag ccggtaccgg cagcgtggcg
     5401 gagtggctca ctggcaagct gaccgtcccg gcggaagagt ttcaccaggc cgacggctcc
     5461 gggatgtcgc gcgaaaatcg cgcgtccgca aacgccttca tccatctgct gcgctacatg
     5521 tggaagagtc cgtaccgcga ggacttcgtc agctcgttgc catactcggg cgatcctgac
     5581 gcgaaattcg gacaccgcct gcgcggcgca ccctacgcgc gtcaggtgta cgcgaagacc
     5641 ggctacatcg tcggcgtggt cggcctttcg ggctacgtgc gcggcgcgag cggcaaggtc
     5701 tatgcgttct cgttcgtctt caacaactac cacgtcgggg tctacgcggt gtaccagctt
     5761 caggattcga tgctgaagga aatcattact agaggatgat tgctctgcgc gtggagcgtt
     5821 gtcggttgtc ggttagatgc gcttcgcgcg ttgtcagaag taacgcgaga tcggctacga
     5881 ttgcgcactg gtcgaacaac tgacatccac tggcttcatg ctgacaacgc gcgaagcgca
     5941 atcaaccgag aaccgacaac gcgcgaagcg catgaaaaaa gaaaccatca tcttcaccac
     6001 ctgcggcttt cttctcggcc tgaccctcgg cgggctggtc atcgggccga agattgcgca
     6061 gtcgaagctt gctggtgcgg cgccggttag cgagacagaa agcgctgctc cggctgcgac
     6121 acctccgatg ggtgcggcgg gcggcaatcc gatggccgct gttcagcaac agctggccac
     6181 tctcaaagcc acgctcgaaa aagatccgaa gaacttcgac gcgctcgtgc agctcggcga
     6241 catgtatatg gacgcggcga agttcccgca ggcgatcgat tatctcgatc gcgccttagc
     6301 cgtacacgac gacgctaacg tccgcaccga cctcggcatc tgctacaaac agacgggcca
     6361 gccggcgagc gctctggctg agtttgagcg tgccgcttcg atggctcccg atcagtggca
     6421 gcctcttttc aatcaggcga tcgtgttgaa cgatctgcat cgcaccgacg aggcccgcgt
     6481 ggt
//
LOCUS       DATLVW010000490         7355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_311225, whole genome shotgun sequence.
ACCESSION   DATLVW010000490 DATLVW010000000
VERSION     DATLVW010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311225"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VNN25_14760"
     CDS             complement(<1..538)
                     /locus_tag="VNN25_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH92841.1"
                     /translation="MLDVIVIGGGHAGAEAARVCARGGARVALVTMLRDAIGRMSCNP
                     AIGGLAKGNLVKEIDALGGMMGEATDQAGIQFKVLNRSKGPAVQGPRAQCDRDLYAAA
                     VQQILAAEPNISIVEGSVSALLFNGRTVRGVAFRDGTRLEARAVIVTTGTFLRALMHS
                     GETQTVGGRFGEPAAETLS"
     gene            complement(975..2294)
                     /gene="mnmE"
                     /locus_tag="VNN25_14765"
     CDS             complement(975..2294)
                     /gene="mnmE"
                     /locus_tag="VNN25_14765"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXH92842.1"
                     /translation="MTTEQPDLDTIVAPATPLGRGALAIVRIDGPGSAAILQTLGGVS
                     PEIRHATHILLQINGEVLDDCVAVRYAAPQSFTGNDLVELTLHGSPLLVQRVIAAVVE
                     LGARIAQPGEFTERAVLNGKLDLVQAESIADLINSRTALQARLSLGNLDGVLSRRAMS
                     IRDTLLHVISRLEAALDFSEEGYEFITREDARARIVDAIAETKAMGETYRRGRATTSG
                     LKAVILGRPNAGKSTLLNRLVGSERAIVTAIPGTTRDIVSETIEIGGLPVTIADTAGL
                     RSSGDVVEEIGVARAREAAGQADIILYLVDASEGVSAEDEMALFPKAILIYTKIDRAA
                     APSGAFGISVKAELGIEQLLARLDALVREEFAAPEGSLVNERQLRAVTECSQALQAAL
                     AAVEGGLDEQIIVVDLNRAASALALLTGAITTGDVFTEIFSKFCIGK"
     gene            complement(2696..3586)
                     /locus_tag="VNN25_14770"
     CDS             complement(2696..3586)
                     /locus_tag="VNN25_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXH92843.1"
                     /translation="MAQRFEGKSLDEALTMATEAFGVERDQLTYHVVLEKRGFLGGFK
                     RIVVEAEVNDAAVASPAHAPAVAQGDSGHGAPSRRASGGGGRGRGRGGRDRGRGRGGD
                     GRGRGGNSGGERGRGPRREHGDDFQTGDFERFAGEIPEQGPESEQAGVVRAWCARVVE
                     LSNLSLEIRTEENETQIIVRLFGADARLLTDQHGELLDAIQVLSNKALVGNTVEKEIE
                     LNCEAFKERRTEQLAEKARDLADEVRTNGREQLLPAMTPIERRIVHLTLQEDADVTTE
                     SRGDGFYKRVAILRRQKQAE"
     gene            complement(3602..5305)
                     /gene="yidC"
                     /locus_tag="VNN25_14775"
     CDS             complement(3602..5305)
                     /gene="yidC"
                     /locus_tag="VNN25_14775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXH92844.1"
                     /translation="MEKRIFAAVLISIGFLWLWAAVAPKLFPDLMKKPVPAVEQPAAT
                     STAASSSAPAPAASPSNSPTTTTTTTATSTTAASTPAVPAPVVQPVEASTQTRTTYAA
                     DNFIARLSNRGGELVSFELKNYRAKPSSKTDTQNVDLVKEREPGRTDYPFAIEATDPV
                     FTHRANAALYAVREFDDKGAHVIEYRWSDGVHAVTKTFRFTREYLFNFAVSVVPPIPY
                     RVVIGPGIRTLGPEEKDNQFITTGNGVYQRDDSLKVIAREKAGSLTPFPSVQYVGIED
                     NYFLSALRPEKSNGALLRAIDVPAGAKNEKRREIFAGVNAAPDGVVTGAAFFGPKEAK
                     VVDLYGFEKTLQLGVFGIIARFFLAALLWLNKFTKNYGFAIILLTLIIKLVLYPLQHK
                     WIVSMKKMQKLQPKMEAIKAKYKKAKTDPEQRQKMNTETMKLYQQEGINPAGGCLPFV
                     LQVPILWGFYGLLTHAIELRGAPFMLWIQDLSMKDPYYITPILMTVTMFIQQAITPST
                     ADPAQKKMFMIMPLIMGWIFKEFPTGVVLYWLMQNVLTIVQQVIMNKFWKDQPAEVQA
                     G"
     gene            complement(5307..5516)
                     /gene="yidD"
                     /locus_tag="VNN25_14780"
     CDS             complement(5307..5516)
                     /gene="yidD"
                     /locus_tag="VNN25_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009545986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXH92845.1"
                     /translation="MRAIAILPLRFYKRFLSPLLPPMCRFDPTCSIFMMQAIEKHGTL
                     RGVWLGLRRLARCHPFNPGGWDPVP"
     gene            complement(5520..5927)
                     /gene="rnpA"
                     /locus_tag="VNN25_14785"
     CDS             complement(5520..5927)
                     /gene="rnpA"
                     /locus_tag="VNN25_14785"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXH92846.1"
                     /translation="MPVKGPLATGAGASDSQALPKEKRLAKRREFLRVYERGRKLFSR
                     YCVVFFAANGLTYSRVGVTATRKSGKANVRNRLKRWTREIYRHQRGPLGLDARAIDIV
                     VNLKPNAAQTAWSEFSPDLQRTLQRVAAESAAR"
     gene            complement(5962..6096)
                     /gene="rpmH"
                     /locus_tag="VNN25_14790"
     CDS             complement(5962..6096)
                     /gene="rpmH"
                     /locus_tag="VNN25_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXH92847.1"
                     /translation="MKRTFQPNNRRRKRTHGFLVRMRTKDGQAVLSRRRRKGRKRLSV
                     "
     gene            6446..>7355
                     /gene="dnaA"
                     /locus_tag="VNN25_14795"
     CDS             6446..>7355
                     /gene="dnaA"
                     /locus_tag="VNN25_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXH92848.1"
                     /translation="MNLWQQVLDRLEQTIERTQYETWFVPTRFLAQKGDTIDVSVPSQ
                     RFVSEIRQNYAQQIRSVLDELSTDHINLHLVADTEGGGDAILSAGPPPAVELQTSIFN
                     PRYRFETFVVGKSNELAFAASKSVAENPAGSFNPLFIYGGAGLGKTHLIQAIAQQLRD
                     TTPGAKIVYTSADSFVTDFITSIRYDRMQQFRDRYRKVDALLLDDIQFMANKEQTQEE
                     FFHTFNALYESRRQIVFTSDAPPKEIPTLEDRLRSRFEWGLIADIQPPDLETKVAILR
                     KKADEKKIDLPHDAALFVAERVRTNIR"
BASE COUNT         1431 a         2265 c         2256 g         1403 t
ORIGIN      
        1 cggacaaggt ctctgcagcc ggctcgccaa aacggccgcc gacggtctgc gtttcgcccg
       61 agtgcatgag ggcgcgaagg aaggtgccgg tcgttacgat cacggcgcgc gcttcgagcc
      121 tcgtaccatc gcgaaacgcg acgccgcgta cggtcctgcc attgaacaga agcgccgaaa
      181 cagaaccttc gacgatgctg atgttgggct cggcggccag aatctgctgg acggcggcgg
      241 cgtagaggtc gcgatcacat tgcgcacgcg gtccctgaac ggcaggacct ttggaacggt
      301 tgagcacttt gaactggatg ccggcctgat cggttgcctc ccccatcatt ccgccgaggg
      361 cgtcaatttc tttgacgagg ttgcctttgg ccaggccgcc gatcgcgggg ttgcacgaca
      421 tgcgtccgat ggcatcgcgc agcatcgtga cgagcgccac gcgtgccccg ccgcgcgcgc
      481 aaacgcgagc agcctccgcg ccggcatggc caccaccgat gacgatgaca tcgagcatgt
      541 ttcacgtgga acatgcggtc tagtctcgtt catcccgagg ctgggggtgg gcgcattgat
      601 actgcgaacg agtcagggcc cagcgattgt cacaaaagtt ggtaatttaa cggctaaaaa
      661 tggaacttag ataaaatgag aatccgttaa cggtaaatgg gaatccgtta acggtaaatc
      721 agaatccgtt aacggtaaat cagaatccat gaacggtaaa tgagaatccg tgaacggtaa
      781 atgggaaacc ctgaacggta aacaggaaac cgtgaatggg aaatgagaat ccatgaatgg
      841 gaaatcagat cacctgcgcg ggaagtcgaa acccgtgaac cggaagagag aacgggttca
      901 cgcggagccg cggagagcgc ggggcttctt tctccggcct ccgcgcctcc gcgtgaccgt
      961 cgccccggcc tcgttcactt tccgatgcag aacttcgaga agatctccgt gaagacatcg
     1021 ccggtggtga tcgcgccggt caggagcgcg agcgcggatg cggcgcggtt gaggtcgacg
     1081 acgatgatct gctcgtcgag ccctccttcg actgcggcaa gcgcggcctg cagtgcttgg
     1141 gagcactccg tcaccgcacg caactgccgc tcgttgacga ggctcccctc cggcgccgcg
     1201 aactcctcgc gaaccagagc gtccagccgc gccagcagtt gctcaatccc gagctcggcc
     1261 ttcacgctaa tcccgaacgc tccactcggg gccgccgccc gatcgatctt ggtgtagatg
     1321 agaatcgcct ttggaaacaa cgccatctcg tcctcggcgc tcactccttc cgaagcatcg
     1381 accagataaa gaatgatgtc ggcctggccg gcggcctcgc gcgcgcgggc cactccgatc
     1441 tcctcgacga cgtcgccact cgatcgcagg ccggctgtgt ccgcgatcgt caccggcagt
     1501 ccgccgatct caatcgtctc ggacacgatg tcgcgcgtcg tccccggaat agcggtcacg
     1561 atggcgcgct cgctcccgac gaggcgattc agcagcgtgg acttgccggc gttcggccgc
     1621 ccgaggatca cagccttcag gccggaggtc gtggcgcggc cgcggcgata ggtctcgccc
     1681 atcgcctttg tctcggcgat cgcgtcgacg atccgcgcgc gggcatcctc ccgcgtgatg
     1741 aactcgtatc cctcctccga aaaatcgagc gctgcctcga ggcgggagat gacatgaagg
     1801 agtgtgtcgc ggatcgacat ggcccggcgg ctcagcactc cgtcgaggtt gccgagggag
     1861 agtctggcct gaagcgcggt gcgggagttg atcagatccg cgatcgactc ggcctgcacg
     1921 agatcgagct ttccgttcaa cacggcgcgc tcagtgaact cgccgggctg ggcgatcctc
     1981 gcgcccaact cgaccacagc ggcgatcacg cgctgcacga gcaatggact gccgtgaagg
     2041 gtgagctcga cgagatcgtt gccggtaaac gattgcggcg cggcgtaacg aacggcaacg
     2101 caatcgtcga gcacttcacc attgatctgc aacaggatat gggtggcatg cctgatctcc
     2161 ggcgagacac ctcccagcgt ttgcaggatg gccgcgctgc ccggcccgtc gatccggacg
     2221 atggcgagtg ctcccctgcc gagcggtgtg gcgggagcga cgatcgtatc gaggtcaggt
     2281 tgttcggttg tcacgttgaa ttgctgggtt gctgggtttg ctcggttgct ggtttgttgg
     2341 ggttgcccaa catgccctaa cttgcatttc tcacacttct gtggagttgg cagcagtacg
     2401 tttccatcga gcgccttctc ccccacgctt tttgtggggg agaaggtggc cgaaggccgg
     2461 atgagggggc gtttgattgt cgcggcacgg ctgccacaaa ccgcaagcga caatcgagac
     2521 ctcttgagaa gaacgtgttc atgcacggcc cccctcatcc gccttcggca ccttctcccc
     2581 ccgaaaagca cggggggaga aggcgctcga ttcaaaggag tcgcttagaa agtgtgagga
     2641 atgctggcca accgaacaac gcagtaaccc agcagcccgc tgaggccacc ggcaactatt
     2701 cagcctgttt ttgccggcgc agaatggcaa cgcgtttgta gaagccgtcg ccacgcgact
     2761 cagtcgtgac atcggcgtct tcctgcagcg tgagatggac gatgcgccgc tcgatcggcg
     2821 tcatcgccgg gaggagctgc tcgcggccgt tggtgcgaac ctcgtcagca agatcgcgcg
     2881 ccttctccgc aagttgttcg gtgcggcgct ctttgaacgc ttcgcaattc agctcgatct
     2941 ctttttccac cgtgttgccg acgagcgcct tgttgctcag aacctggatg gcgtcgagca
     3001 actcgccgtg ctggtcggtg agcagccgcg catcggcgcc gaacagccgc acgatgatct
     3061 gggtctcgtt ttcttcagta cggatctcga gcgacaggtt cgacagctcg accacacgcg
     3121 cgcaccaggc gcgcacgacg ccggcctgtt ccgactccgg tccttgctcc gggatctcgc
     3181 ccgcgaagcg ctcgaaatcg ccggtctgaa aatcgtcgcc gtgctcgcga cgcggtcccc
     3241 ggccacgctc accgcccgag tttccgcctc gacctcgacc gtcgccgccc cgccctcttc
     3301 cgcggtcccg gccaccgcgg ccacggccgc gaccaccgcc accgctcgcg cggcgcgatg
     3361 gcgcgccatg gcccgaatcg ccctgagcaa cagcgggcgc gtgtgccgga ctggccaccg
     3421 cggcgtcatt cacttcggct tcgacgacga tgcgcttgaa cccgccgagg aagccgcgct
     3481 tttcgaggac gacgtgatac gtcagttgat cgcgctcgac cccgaacgct tcggtggcca
     3541 tcgtcaacgc ttcgtcgaga ctcttacctt caaatcgctg agccattgat cttccgctcg
     3601 atcaaccagc ctgcacttcg gccggctgat ccttccagaa tttgttcatg atgacctgct
     3661 gcacgatggt caatacattc tgcatcaacc agtaaagcac gacgccggtc ggaaactctt
     3721 tgaaaatcca tcccatgatc aacggcatga tcatgaacat cttcttctgc gccggatcgg
     3781 cggtcgatgg cgtgatggcc tgctggatga acatcgtcac cgtcatcagg atcggcgtga
     3841 tgtagtaggg atccttcatc gagaggtcct ggatccagag catgaagggc gcgccgcgaa
     3901 gctcgatggc gtgcgtcagc agaccgtaga agccccagag aatcgggacc tggagaacga
     3961 acgggaggca gccgccggca ggattgatgc cttcctgctg gtagagcttc atcgtctccg
     4021 tgttcatctt ctggcgctgc tcgggatcgg tcttggcctt cttgtatttg gccttgatcg
     4081 cctccatctt cggctgcagc ttctgcatct tcttcatcga gacgatccac ttgtgctgca
     4141 gcggatagag cacgagtttg atgatgagcg tcaggaggat gatggcgaag ccgtagttct
     4201 tcgtgaactt gttcagccag aggagagcgg ccaggaagaa gcgggcgatg atgccgaaga
     4261 cgccgagctg gagagtcttc tcgaagccat agagatcgac gaccttggcc tctttcgggc
     4321 cgaagaaagc cgcgccggtg accacgccgt cgggcgcagc gttgactccg gcgaagatct
     4381 cgcgccgctt ctcattcttg gcgccggcgg gaacgtcgat ggcgcgcagc agcgcaccgt
     4441 tcgatttctc cggacgaagg gcgctgagga agtagttgtc ctcgatgccg acgtactgca
     4501 cggaaggaaa aggcgtcagc gagccggcct tctcgcgggc gatgactttg aggctgtcgt
     4561 cgcgctgata gacgccgttc cccgtggtga tgaactggtt gtccttctcc tcgggaccga
     4621 gcgttcggat cccgggcccg atgacgacgc ggtacggaat cggaggaacg accgacaccg
     4681 cgaagttgaa gaggtactcc ctggtaaagc ggaaggtctt cgtgaccgcg tggacgccgt
     4741 cggaccagcg gtactcgatg acgtgagcac ctttgtcgtc gaattcgcgg accgcgtaaa
     4801 gcgcagcatt cgcgcgatgg gtgaagacgg ggtccgtagc ctcgatggcg aagggatagt
     4861 cggttcggcc gggctcgcgc tccttgacga gatcgacatt ctgcgtgtcg gtcttcgagc
     4921 taggcttcgc cctgtagttc ttgagctcga aggagaccag ctccccgcct cgattggaca
     4981 accgggcgat gaaattgtcc gcggcatagg tcgtccgcgt ctgtgtggag gcttcgaccg
     5041 gctgcaccac cggagcgggg accgcaggag tgctcgccgc agtcgtgctc gtggccgtag
     5101 tcgtcgtggt cgtcgtcgga gaattcgaag gactcgcagc aggagctggc gccgaagagg
     5161 aagcggcagt tgacgtggca gccggctgct ccaccgccgg aaccggcttc ttcatcagat
     5221 caggaaacag cttcggagcc acagcggccc agagccagag gaaaccgatc gagatcagta
     5281 cagcagcaaa gattcttttt tccatctcaa gggacggggt cccaacctcc aggattgaac
     5341 gggtggcagc gggcaagacg ccgcaggccg agccagacgc cgcgcagtgt gccgtgcttt
     5401 tcgattgcct gcatcatgaa aatggagcag gtgggatcga agcggcacat cggcggcagc
     5461 agcggtgaga gaaatcgctt gtagaaacgg agcgggagga tggcaatggc gcgcatgact
     5521 catcgcgccg cgctttccgc ggcaacgcgc tgcagagtgc gctgaaggtc ggggctgaac
     5581 tcgctccacg ccgtctgcgc cgcgttcggt ttcaggttga cgacgatgtc gatcgcgcgc
     5641 gcgtcgagac ccagcgggcc gcgttgatgg cgatagattt cgcgcgtcca ccgtttcaat
     5701 cgattgcgca cgtttgcttt gccgcttttc ctggtcgcgg tgacgccgac ccgcgagtac
     5761 gtcaggccgt ttgctgcaaa aaagactacg cagtagcggg aaaagagttt gcggccacgc
     5821 tcataaacgc gaagaaactc gcgtcgcttg gccaacctct tctctttggg cagcgcctgt
     5881 gaatcggagg cgccggcacc ggttgccaaa ggccccttca ccggcatgat gcctccggaa
     5941 ccttctggac acaccggctg attagaccga aaggcgctta cggcccttgc ggcggcggcg
     6001 cgaaagcact gcctgcccgt ctttggtccg catccggacc agaaagccgt gcgtgcgctt
     6061 gcggcggcgg ttgttcggtt gaaaggtacg cttcatgatt tcgttaaccc tctgaaaaga
     6121 aagcggcgag tatagcggcg ggtcggaaag tcgtcaaacc agccagcgac cgggcggaac
     6181 gtggggtggt gatgggctat tccgaggggc ggttgaggac tcaaaggcat cactcaaaga
     6241 catcaccaca gagacacaga ggacacagag aaacctctgt gatctctgcg tctctgtggt
     6301 tcatgattct ctgatcgccg cgcctgcaaa accacgcgac cgcacacgcg atccgtgcta
     6361 tattccgctc ccctttctac cgtccggatt ttccacagtt gtggaaaagt tgtggattct
     6421 cagaaagata ggccgcgccc ctccgatgaa tttatggcag caagtgctcg accggctgga
     6481 gcaaaccatc gaacggacgc agtacgaaac gtggtttgtt cccacccgct ttctagccca
     6541 gaagggtgac acgatcgatg tcagcgtacc gtcgcagcgc ttcgtaagcg agatccgcca
     6601 gaactacgcg caacagatcc gctcggtcct cgacgaactg tcgaccgatc acatcaatct
     6661 tcacctcgtg gcggacaccg agggtggcgg tgacgccatt ctctccgcgg gcccgccgcc
     6721 ggccgttgag ctgcaaacct cgatcttcaa cccccgctat cgcttcgaga cgttcgtggt
     6781 cggcaagtcg aacgagctgg ccttcgccgc ctcgaagtcg gtcgccgaga atccagcggg
     6841 ctccttcaat cctctcttca tctacggggg tgccggtctg ggaaagacgc acctcattca
     6901 agcgatcgcg cagcaacttc gcgacacgac tcccggcgcg aagatcgtct acacatcggc
     6961 cgattctttc gtcaccgact tcatcacctc gattcgctac gaccggatgc agcagttccg
     7021 cgaccgctac cggaaggtcg acgctctcct cctcgacgac atccagttca tggccaacaa
     7081 ggaacagacg caagaggagt tcttccacac ctttaacgcc ctctacgagt cgcgaaggca
     7141 gatcgtcttc acctccgatg cgccgccgaa agagatcccc acgctcgaag accgccttcg
     7201 ctcccgcttc gaatggggat tgatcgccga catccagcca cccgatctcg agacgaaggt
     7261 tgcaatcctt cgaaagaaag ccgacgagaa gaagatcgac cttccgcacg atgccgcgct
     7321 cttcgtcgcc gagcgggtac ggacgaacat ccgcg
//
LOCUS       DATLVW010000491        14524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_557107, whole genome shotgun sequence.
ACCESSION   DATLVW010000491 DATLVW010000000
VERSION     DATLVW010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557107"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VNN25_14800"
     CDS             <1..215
                     /locus_tag="VNN25_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH92849.1"
                     /translation="SIEQPKLIATAGELLIELVQRMQQNGTDRSPVIDNEESRRVIGF
                     ISPSDILRVRLRRTTGDDESPFELFE"
     gene            complement(748..3447)
                     /locus_tag="VNN25_14805"
     CDS             complement(748..3447)
                     /locus_tag="VNN25_14805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXH92850.1"
                     /translation="MTSTPLRYAAAIAMGIASILIQLAAPSVFQAMPFFLFFVAVFLS
                     ALVAGLGPAIVETVFATLLTSYFFHPSAVQTGLFFVTGIVVSWFVSSNRRWEEAQKNA
                     EVDHLTTIANVAPAIVWIADAGKKCTYVNDRWLEFSGRTREEELGFGWLSEMHEEDIP
                     ACMERFERAFESREPVDIEYRHRRHDGVYRWMLSRGVPRTDRNGVFAGYIGVALDIDD
                     LRASEKEQKRLALAVGRERERLLGLVSNVPGVVWETWGRPDDGSQIIDFVSEHVERML
                     GYTVEEWLTTPNFWLTIVHPDDREAAAKRAHEHFLAGGARSNTFRWMTKDGRAIWCEA
                     HSTVIQDEDGNPVGMRGVTLDISTRRHAEASLRFAARAGETLASSIDYDATLKATAGL
                     AVPELADWCVVVLVDEDGQTHRAAVVHADPKKNELAQKLLTIPRRQDMPPAIAAQFAK
                     MQPFILEMTDEVIRSTAYDDAQAALVRELGAATTLIAPIESQNRYLGTISFVSATPGR
                     YDERDIELGLLLARRVATAIDNAQLYRAAVHASTAKDEFLATVSHELRTPMTATLGWV
                     RLLSLGNVDAETQKTGLHAIENSTRAQAKLIDDILDVSTIILGKFRLDRGPVDLRAVI
                     DSATEALKPALAAKSIAMDVDTSRWTGAIEGDASRLQQVFWNLLANAVKFARRDGRIE
                     VILERDDGVARITVRDDGAGIDPAFLPHVFDRFRQAEGGSTRKHGGLGLGLAIVRHLV
                     ELHGGNVQASSEGRGKGASFVVELPATLEVERTNRIDVAALPDLGRKQILVVDDEQPT
                     LDLVATLLRHCGARVTTARSADEAMVALNAGEHDLLITDIAMPGTDGLAMMGRVREMK
                     PSLPAIALSARSDIPGTGFARVLQKPIDPIELASQIAQVIASK"
     gene            complement(3541..4185)
                     /locus_tag="VNN25_14810"
     CDS             complement(3541..4185)
                     /locus_tag="VNN25_14810"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXH92851.1"
                     /translation="MPDLTDLQDHILRLFFDRRDEFFLTGGAALVGFYLHHRETHDLD
                     LFTEVEAIDEGERTLREIGESLDLEIETLRRSPDFRRFLLRGSSEGVVVDLVKDVSPQ
                     LLDKISIGRIVIDSAREILANKLCALLSRTEVRDLVDVARLEEAGNDPMAVLGLAFQK
                     DAGMSAGQLAWVLSSFPIPTDPSPLYGMSQKDLEDFRESLVSRLTAAAFPRPPL"
     gene            complement(4186..4509)
                     /locus_tag="VNN25_14815"
     CDS             complement(4186..4509)
                     /locus_tag="VNN25_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92852.1"
                     /translation="MGRRSDAAASSGVALTTDFSSEEAVPYFLWDEPMTLRELRARLK
                     RSDEDRVRLLGKILREAREPDIWLFTTAEEVSSRWDQLERLLGRRREFWHFLLDRWRE
                     QGLLR"
     gene            complement(4713..6389)
                     /locus_tag="VNN25_14820"
     CDS             complement(4713..6389)
                     /locus_tag="VNN25_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH92853.1"
                     /translation="MPHDDEEERLRSVAIQNSQSILLARQRAEQELVTAKEALELRTE
                     ELERANELIRTIAENAASGLLMLDNRGVPTYMNPAAIEETGRTLEEMALASLHDVLHA
                     PVERGGHPAAECPIRNARKRMTPLKNHRDVFVRKDGSTFPVSCAVSPLQRDGHAAGAV
                     LEFRDITDELLAQKTLEDANRRKDQFLATLSHELRTPMTAVLGWARMLKLGLSESETR
                     EALDAIEKSAEIQAQLIDDVLDVSRIVAGKMTFKPVPIDVSSVLHAAMTTVHPAAAAK
                     GIEILASVTPRLPNILGDEGRLQQVIWNLLSNAVKFTPIGGAITVRLVQTGSILRLTV
                     QDNGKGIEPSYLPHVFEPFSQEDGTMTRSHEGIGLGLSIVCSLVELHGGRIRVASEGA
                     GRGATFTVEFPIMEPTPVLKRIAPRTEVPFATAAASELPKLTGIKILVIDDQVLTREL
                     VTAVFRRADADVSVAASVREGLALFRTRSPDIVVCDLAMPEEDGFAFVRAVRAMPAPA
                     NATPVIAMTAFGRPEERQSALAAGFDEYLKKPIDPLELASTVLRSLTPRR"
     gene            complement(6399..7463)
                     /locus_tag="VNN25_14825"
     CDS             complement(6399..7463)
                     /locus_tag="VNN25_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HXH92854.1"
                     /translation="MTIPTEPIGSIPRPLELIEAIERSGDANDPALDPLYDAAIRDTL
                     ARFEATGSPVITDGEQRKYHNFWTYSVHGLPNTSPDGFKIPFSAGHTRRMPRLTSGPF
                     RYLRLADSYLDVARRYTNLPIKQAVISPSALSLMYPPEGIEGYTPEEFIDDLLIEHET
                     EVRRCLAKGAVKVQIDFTEGRLAVKIDPSGNLLNSFIDLNNLVLSRFSAEDRAKIGVH
                     TCPGGDLDSTHSAEVDYADLLPSLFQLKVGNFCIALAGEKDRPRVLKIIRKYLKPDQR
                     VFVGVVAPIDPHIETAEEVMERVLEAAEYIPVAQLGTTDDCGFSPFSDDTSTTRDTAF
                     AKIRARVDGTALAAERLGGH"
     gene            complement(7595..8800)
                     /locus_tag="VNN25_14830"
     CDS             complement(7595..8800)
                     /locus_tag="VNN25_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH92855.1"
                     /translation="MRTLLLALLFATTAAAQPIVIRAARVIDGRGHVVEDAAVVVDGA
                     KIVRIVPHASALKDVTFDLGDRTIMPGGVDTHVHIGWHFDAKDGRSHDDETDRNETPQ
                     EAVLYAAENAVRTLEGGITTVQSLGAPVDKPLRDAIARGTLPGPRILTAIEPIFDNKL
                     TPEALRAAVVKLAGEGADVVKVFASESIRSGGGPTMSQEQMDAICGEAKKQHLRVAVH
                     AHGPESVRRAVLAGCTSIEHGALIGQPELDLMAAHGTYFDPNIDLVFRNYFENKSHYL
                     GIGNYTEEGFAQMKLAVPKALHVFQLGLATPGLKIVFGTDAVAGSHGRNFQELEYRVT
                     TGGQKAMDAITSATSLAAESLGLGDRVGTIAAGYEADLIAVDGNPLRDINALSHVSFV
                     MKSGRVIRR"
     gene            9481..9678
                     /locus_tag="VNN25_14835"
     CDS             9481..9678
                     /locus_tag="VNN25_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92856.1"
                     /translation="MKPSDKTAPEHGARRDLFNELTEGMAALSLLRGQGLRPGVNLDD
                     SAALLELMESDRDSDLLLSDL"
     gene            9729..10793
                     /locus_tag="VNN25_14840"
     CDS             9729..10793
                     /locus_tag="VNN25_14840"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HXH92857.1"
                     /translation="MQILARAYRGDHVESFHTGSIAVVDSLGRLVAYTGEPGLRTCLR
                     SAAKPFQAIPLLEYGGLEEYELSGEELALTCSSHGGEPVHVATAAALLRKGEFDETDL
                     LCGAHMPYDEKSAAELRASGDEPSPLHNNCSGKHAGMLLATQVMDVPAARYIDSDHPL
                     QETMRATLSDFAGLDPEEIPIAVDGCGVPAFFLSLHRAAFAYARLMATSAGAAPGALQ
                     RYSTSTEHIVGAMTSFPYYVAGAWSITTPLMKAFDGELLAKEGAEGFYTMALAPSLAS
                     TLTGRLGLADDVSLGIALKIHDGSMVRGRYPVILRTLELLGVDIAMAPDLQQFRKLPV
                     ANVAGAIVGEVRAEFDLEIL"
     gene            10823..11260
                     /locus_tag="VNN25_14845"
     CDS             10823..11260
                     /locus_tag="VNN25_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH92858.1"
                     /translation="MVTNRVGVQIGGALLLSALCLLPSCSTVGVSKTTGGFSEISPAV
                     AAEMIIDSQQIVVIDVRSTADYRGPEGHIAGAINAPFDVIETRLPELLPYQNQTVLVY
                     GETSTDGAVAAQLLTVAGFRNIVHVTGGIKNWIEHGYRTVNAQ"
     gene            11290..12363
                     /locus_tag="VNN25_14850"
     CDS             11290..12363
                     /locus_tag="VNN25_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXH92859.1"
                     /translation="MKRVLMATAVALLPFCAQAQMAPSLSVALKNFAVRSSAQCPDNR
                     IAVDPVTGVNGGPAGFSLYELTQESSDPSCQRKTYLLFSPATSQVLIGTIFGLEQDGK
                     TPAAKIVEVAGAALKSKVTATIAPFPLPDGIHAVEITKQSKEAGGAFNYHGFLDASNN
                     FLIVGTRGNLRSDPGKAMIEAIGLTGAMRRGNPKSRVQIIELSDFECPTCARAHKKVE
                     PIIAKNLDKVDYYRLDLPLFEHHEWALSAALGARALAKVAPSKYWSYVNFVFENQETI
                     GKQPFDTFLKNFVDDNDVNWKAVEKIYRSPAEKRALIDQVSRAFDNGIVSTPTYIING
                     QILGFGPEGSFTIDAIKKALGVK"
     gene            complement(12442..13977)
                     /locus_tag="VNN25_14855"
     CDS             complement(12442..13977)
                     /locus_tag="VNN25_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HXH92860.1"
                     /translation="MKRFIAIVLFLTASVACADEYRAYWVDTFRTPFATPADVKRIVD
                     AAVDSNANALFVQVRRRGDAWYLDASEPLAETLDGTFDPLRAMLDLAHSRGIQVHAFV
                     IVGAVFRGDPAVDPVPRDPHHVFLQHFWDAKTHKPFRGPRQWGTRTSKGRYLFSSDWY
                     ADLGHPQAAAYTADVLLHLVRAYDIDGLHLDRVRYPESPHADVGYNETSVARFRARYG
                     RLPRAKDPLWNDWRREQVTNFVRRVSLGAKAIRPSITISAALISWGTGPGANGGFKNT
                     DAYRVAFQDWEGWLREGIIDAGSPMLYKREHVPAEKKQFDDWLQFLVTTAHTNRRTAL
                     PGIGAYLNSIEGTLRQARRARAANADGIIFFAMGDTKPWSILANSTNDAVRHNPYAIP
                     VAGIYTPKRPNEEFFLALRKSTDGQTAFERTDLPLFAATLPAPRQAEEANGAVMGYAE
                     GDGTLVTIESLATLDTRTTRTDGNGFFGMLGLAPGEYRVVAGASAATVTIAAGKVAVW
                     SPA"
     gene            14074..>14524
                     /locus_tag="VNN25_14860"
     CDS             14074..>14524
                     /locus_tag="VNN25_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXH92861.1"
                     /translation="MHINKPPAGNRPLKTLERVLSKAGLGSRTEARTWIGGGRVQVNG
                     QVVRDPDAWVDLELDRIEFVGKPLRSEQRVYVLLHKPAGYLTTYRDPQSRPTIYDLLP
                     DRERYLVHVGRLDFDTSGLLILTNDTQFAERMTNPEYHIPKTYLVEAS"
BASE COUNT         2763 a         4548 c         4561 g         2652 t
ORIGIN      
        1 ccagcatcga gcaaccaaag ctaatcgcca ctgctggcga gctgctcatc gagctcgtgc
       61 aacgcatgca acaaaatgga accgaccgct ctccggtcat cgacaacgaa gaatcgcgaa
      121 gagtgatcgg cttcatcagc ccatcggaca tcctgcgcgt acggttgcga cggacgacgg
      181 gagacgatga gtcgccgttt gagctgtttg agtgagactc ttccctttcc cgccaggact
      241 gcgcaatcga ggtggcgaaa cgcggctaac cccgctaccc gcatttctca cacatttctg
      301 cgacaccttc caatcgagcg ccttctcccc ccgcgctttt tttgcggggg gagaaggtgg
      361 cgaagccgga tgaggggggc gttgcaaggt gggacaaaca cgttgtttgc gagggtgact
      421 tgactgaaaa ggcgtccttt agtgtttgcg cgacaggcaa acgccccctc atccggcctt
      481 cggccacctt ctcccccact gagaggcgtg ggggagaagg cgctcgattg agagcacggt
      541 gccggcaatt ccagtgcaag tgtgaggaag gtgagacggg ccagccggaa aaggtcacgc
      601 aaccggggga aagcaccaaa agacgggaaa gggcggtaat aagtcggaat gaaacggatc
      661 gatgccggtc caaatcggat cgatgccggt ccaaatcgga ccaatgcaca tcgatgttcg
      721 ttgagtccgc gcgaggatcg atcccgctca cttgctcgcg atgacttgcg cgatctgcga
      781 ggccagctcg atcggatcga tgggcttctg cagaacgcgc gcgaagcccg tgccggggat
      841 gtcgctgcgg gcgctgaggg cgatggcggg gagcgacggc ttcatctcac ggacgcgacc
      901 catcatcgcc aggccgtccg tgccgggcat cgcgatatcg gtgatcagca ggtcgtgctc
      961 gcctgcgttg agcgcgacca tcgcctcgtc ggcggatcgg gccgtcgtca cccgggcgcc
     1021 gcaatgacgg agcaaggtgg ccacgagatc gagcgtcggt tgctcgtcgt cgaccaccag
     1081 gatctgcttc ctgcccaggt cgggcagcgc tgccacatcg atccgattcg ttctctcaac
     1141 ttcgagcgtg gccggcagct cgacgacgaa ggatgccccc ttgcctcgtc cctcgctcga
     1201 tgcctgaacg ttgccgccgt gaagctcaac gaggtggcga acgatggcga ggccgagacc
     1261 aagccctcca tgctttcgcg tcgatccccc ctcggcctga cgaaagcggt cgaagacatg
     1321 cggcagaaac gccggatcga tgccggcgcc gtcatcgcgg acggtgatgc gcgcaacgcc
     1381 gtcgtcgcgc tccaggatga cttcgatgcg cccgtcgcga cgggcgaact tcacggcgtt
     1441 ggcgagcaga ttccagaaga cctgctgcaa ccggctggcg tcgccctcga tggcaccggt
     1501 ccagcgcgat gtgtcgacgt ccatcgcgat cgactttgcg gccagggccg gcttcagcgc
     1561 ctcggtggcg gaatcaatga ccgcgcgaag atcgaccggc cctcgatcga gacggaactt
     1621 gccgaggatg atcgtcgaga cgtcgaggat gtcgtcgatg agcttggcct gcgcgcgcgt
     1681 ggagttctcg atggcgtgca ggccggtctt ctgcgtctcg gcgtcgacat tgccgaggga
     1741 cagaagacgc acccatccaa gggtggcagt catcggcgtg cgtagctcgt gcgacaccgt
     1801 ggccaggaac tcgtctttgg cggtgctggc atgaacggcg gcgcgataaa gctgtgcgtt
     1861 gtcgatggcg gtggccacgc gccgcgccag aagcaaaccg agctcgatgt cgcgctcgtc
     1921 gtaacgtccc ggcgtggccg agacgaagga gatcgtgccg aggtagcggt tctgcgattc
     1981 gatcggggcg atgagggtcg ttgcagcgcc gagctccctc acgagcgcag cttgagcgtc
     2041 gtcataggcc gtgctgcgga tcacctcatc ggtcatctcc aggatgaatg gctgcatctt
     2101 cgcgaactgc gcggcaatcg cgggcggcat gtcctgacgc cgcggaatcg tcaggagctt
     2161 ctgggcgagt tcgttcttct tcggatcggc gtggacgacc gcggcgcggt gcgtttgtcc
     2221 gtcttcgtcc acgagcacga cgacgcacca atcggccagt tccggcacgg ccagtccggc
     2281 agttgccttg agcgtcgcgt cataatcgat ggacgaagcg agcgtctcgc ccgcccgcgc
     2341 ggcaaaccgc agagatgcct ccgcatgtct gcgcgtcgaa atgtcgagcg tgacgccgcg
     2401 catgccgacc ggattgccat cctcatcctg aatgaccgtg gagtgcgcct cgcaccagat
     2461 ggcgcgaccg tccttcgtca tccaacggaa ggtgttcgag cgcgcgccgc cggcgaggaa
     2521 gtgttcgtgc gcacgtttgg cagcggcctc gcggtcatca ggatgcacga tcgtgagcca
     2581 gaagttcggc gtcgtgagcc attcctcgac ggtatagccg agcatgcgct cgacgtgctc
     2641 gctgacgaag tcgatgattt gcgatccgtc gtccggcctt ccccacgttt cccagacgac
     2701 gccgggaacg ttcgagacga gaccgagcag gcgttcgcgc tcgcggccga cggcgagggc
     2761 gaggcgcttc tgttctttct ccgaggcgcg aagatcatcg atgtcgagag cgacgccgat
     2821 gtagccggcg aaaacgccat tgcggtcggt gcggggaacg ccgcggctca gcatccaacg
     2881 ataaacgccg tcgtggcggc ggtgccggta ctcgatgtcg actggctctc gcgactcgaa
     2941 ggcgcgctcg aagcgctcca tgcacgcggg gatgtcttcc tcgtgcatct cgctcagcca
     3001 gccgaagccg agctcctctt cgcgcgtgcg gccgctgaac tccagccagc ggtcattgac
     3061 gtacgtgcat ttcttcccgg catcggcgat ccagacgatg gccggagcga cgttggcgat
     3121 cgtggtgagg tgatcgactt ccgcgttctt ctgcgcctcc tcccatcgcc tgttcgagct
     3181 gacgaaccac gaaacgacga tgccggtgac gaaaaacaac ccggtctgca cggcactcgg
     3241 gtggaagaag tacgacgtca gcagcgtggc gaagacggtt tcgacgatcg ccggaccaag
     3301 ccctgcaacg agcgccgaaa gaaacacggc gacgaagaaa aggaagaacg gcatggcctg
     3361 aaaaaccgac ggggcggcca attgaatgag gatcgaggcg atacccatcg caatcgcggc
     3421 ggcgtaacgg agcggggtgg aggtcatcgg gcgaattccg agtttagcgt tagttgaacg
     3481 cgtaatcagc gtcggtggcg gggcgaagac tcgctccagc ggagtgaaga tgatcaatca
     3541 tcatagcggt ggcctcggaa acgctgcggc tgtcagcctg cttaccaggg actcccgaaa
     3601 atcctcaaga tccttctgac tcatgccgta caacggcgaa ggatcagtag ggatcgggaa
     3661 actcgaaagg acccatgcaa gttgtcctgc agacattcca gcatcctttt ggaaggcgag
     3721 tccgagcacg gccattggat cgttgccagc ttcttccagc cgagcgacgt cgaccagatc
     3781 gcgcacctca gtgcgggaca gcaaggcaca taatttgttc gcaagaatct ctcgcgctga
     3841 atcgatgacg attcgaccaa tggatatctt gtccaacagt tgcggcgaaa catcctttac
     3901 gagatcaacc accacgcctt cggatgagcc ccgcagaagg aatcgtcgaa aatcaggaga
     3961 ccggcgtaac gtttcgatct cgagatcgag gctctctccg atctcccgta aagtacgttc
     4021 tccttcgtcg atcgcctcca cctcggtgaa tagatcgaga tcatgggtct cgcgatgatg
     4081 gagataaaac cccaccaagg cggcgcctcc agtcaggaag aactcatcgc gacgatcgaa
     4141 gaagagccgc aggatgtgat cctgaagatc agtcagatct ggcattcagc gcagcagccc
     4201 ctgctctcgc caccgatcga ggagaaaatg ccagaattcc cggcgccgtc cgagaagacg
     4261 ctcgagctga tcccatcggc tgctcacttc ctcggcagtg gtgaacagcc agatatcggg
     4321 ctcccgggcc tcacgcagaa tcttccccaa gagcctgacc cgatcctcgt cagaacgttt
     4381 gagtcgcgca cgaagctcgc ggagcgtcat cggttcgtcc caaaggaagt acgggaccgc
     4441 ctcctccgag gagaagtcgg tcgtcaatgc gacccccgaa ctggctgcag catcacttct
     4501 tcggcccatc atcgaacatc ctagactagg ccatgcaacc attcaattgg atcaaccgtg
     4561 aacatcgctg gcaaggcgac ttcgcatgaa gcgtctgctc gatcagacgt atcgttctcg
     4621 atcgagctca tccgcgaatg aagcttgtcc ttttggacgt tctacgtccg agcgcaccac
     4681 gtggttaacc ttacctggga gtgcctcaca tattaacggc gcggcgtcag cgaacgcagc
     4741 acagtcgagg ccagttccag cgggtcgatc ggctttttca ggtactcatc gaagccggcg
     4801 gcgagcgcgg attgccgctc ttccggccgg ccgaacgcgg tcatcgcgat caccggagtc
     4861 gcattcgcag gcgcaggcat cgcgcggacc gcacgcacga acgcgaatcc atcctcctcg
     4921 ggcatcgcaa gatcgcacac gacgatgtcg ggactccggg ttcggaagag cgccaaccct
     4981 tcgcgcacgg aggcggccac ggagacgtcg gcatcggcac gacggaacac ggccgtcacc
     5041 agctcgcgtg tcaacacctg atcgtcgatg acgaggatct tgatgccggt cagcttcggc
     5101 agttccgacg ccgcagccgt cgcgaacgga acctcggtgc gcggcgcgat gcgcttcaaa
     5161 acgggcgtcg gctccatgat cggaaactcg actgtgaagg tggcgccgcg gcccgcacct
     5221 tcgcttgcca cgcggatgcg tcctccgtga agctcgacca gcgagcagac gatcgagagg
     5281 ccgaggccga tcccttcgtg cgagcgcgtc atcgtcccgt cttcctggct gaacggctcg
     5341 aagacatgcg ggaggtaact gggctcgatc ccttttccgt tgtcctgcac ggtgaggcgc
     5401 aggatggaac cggtctgcac gagacggacg gtgatggcgc cgccgatggg cgtgaacttc
     5461 acggcgttgg acagcaggtt ccagatgacc tgctgcaagc ggccctcgtc gccgagaatg
     5521 ttgggcagcc gcggcgtcac gctggccagg atctcgatcc ctttcgcggc cgcggcagga
     5581 tgcaccgtcg tcatcgccgc atggagaacg gagctgacgt cgatcggtac cggcttgaag
     5641 gtcatcttgc cggccacgat ccgggaaacg tcgagcacat cgtcgatgag ctgggcctga
     5701 atctcggcgc ttttctcgat ggcatcgagt gcctcgcgcg tttcgctttc cgagagcccc
     5761 agtttgagca tgcgcgccca tccgagaacg gcggtcatcg gcgtgcggag ctcgtgggag
     5821 agagtggcca ggaattgatc cttgcgccgg ttggcgtctt cgagcgtctt ctgcgcgagc
     5881 agctcatcag tgatgtcgcg aaactccagg acggcgccgg cagcgtgtcc atcccgttgc
     5941 agcggcgata cggcgcacga gaccgggaac gtcgagccat ccttgcgcac gaacacgtcg
     6001 cgatgattct tgagcggcgt catcctcttg cgcgcattgc ggatcggaca ctccgccgcg
     6061 gggtggcctc ctcgctcgac cggcgcgtga agaacgtcgt ggagcgaagc caaggccatc
     6121 tcttccagcg ttcggccggt ctcttcgatg gccgcaggat tcatgtaggt gggcacaccg
     6181 cggttatcga gcatgagcag ccccgatgcg gcgttctcag cgatggtgcg gatgagctca
     6241 ttcgccctct ccagctcctc ggtgcgcaac tccagcgctt ccttggccgt gacgagctcc
     6301 tgctcggcac gctgccgcgc cagcaggatg ctttgcgagt tttgaatcgc gacggaacgg
     6361 agccgctctt cctcatcgtc gtgtggcaac gctaccaatc agtgccctcc gagccgttct
     6421 gctgcgagag cggtgccgtc gacccgggcc cggatcttcg caaaggccgt gtcacgcgtc
     6481 gtcgaggtgt catcactgaa cggcgagaag ccgcaatcgt cggtcgtgcc gagctgagcg
     6541 accggaatgt actccgccgc ttcgagcaca cgctccatca cttcttccgc ggtttcgatg
     6601 tgaggatcga tcggtgcgac gacgccgacg aagacgcgct gatcgggctt cagatacttg
     6661 cggatgattt tgaggacgcg cgggcgatcc ttctcgccgg ccagcgcgat gcagaagttc
     6721 cccaccttca actggaacag actcggaagc aggtcggcgt agtccacctc agcgctgtgc
     6781 gttgagtcga ggtcgccgcc cggacaggtg tgcacgccga tcttcgcgcg gtcctccgcc
     6841 gaaaagcggg agagcacgag gttgttgagg tcgatgaaac tattgagcag gtttcccgag
     6901 ggatcgatct tgaccgccag ccgtccttcg gtgaagtcga tctgcacttt gaccgcccct
     6961 ttggccagac agcgccggac ctccgtctcg tgctcgatca gcagatcgtc gatgaattct
     7021 tcgggcgtgt agccttcgat gccttccggt ggatacatga gactgagcgc cgagggggag
     7081 atgacggcct gcttgatggg aagattcgtg taacgccgcg cgacatcgag atagctgtcg
     7141 gcaaggcgga gatagcgaaa gggaccggat gtgagccgtg gcatgcgcct tgtgtgaccg
     7201 gcagagaacg ggatcttgaa gccgtccggc gacgtgttcg gaagtccgtg caccgagtag
     7261 gtccagaagt tgtggtactt ccgctgctcg ccgtcggtga tcaccggcga gccggtggcc
     7321 tcgaagcgcg cgagggtgtc gcggatggcg gcgtcgtaaa gaggatcgag tgccggatca
     7381 ttggcatcgc cggagcgctc gatggcctcg atcagctcga gagggcgcgg aatgcttcca
     7441 atcggttcgg tgggaatcgt gatcatgggc gctcctcata tttccggaaa cccagagcgg
     7501 cctctgcaaa ggacagaccg gtttgttgat ccgcagatgt cgcagataga cacagatgaa
     7561 aaccagtcgt ggctctatct gcgcttcctc tgcgtcatct gcggatcact cttccgctct
     7621 tcatcacgaa cgacacatga gaaagcgcgt tgatgtcgcg cagggggttg ccgtcgaccg
     7681 cgatcagatc ggcctcgtaa ccggcggcga tcgtcccgac gcgatcgccg aggccgaggg
     7741 actccgcggc caacgacgtc gccgacgtga tcgcgtccat cgccttctgc ccgccggtcg
     7801 tgacacggta ttcgagctcc tgaaagttcc ggccgtgcga tcccgccacc gcgtcggttc
     7861 cgaaaacgat cttcagcccc ggcgtcgcca gcccgagctg gaagacgtgc agcgctttcg
     7921 gcaccgccag cttcatctgc gcgaatcctt cctcggtgta gttgccgatg ccgaggtagt
     7981 gggacttatt ctcgaagtaa ttgcggaaga cgaggtcgat gttgggatcg aagtaggtgc
     8041 catgcgccgc catcagatcg agctccggct gcccgatcag cgcgccgtgc tcgatcgacg
     8101 tgcagccggc gagcaccgcc cgccgcacgc tctccggtcc atgcgcgtga acggcaacgc
     8161 gaaggtgctg cttcttcgct tcgccgcaga tcgcatccat ctgctcctgc gacatcgtcg
     8221 ggccgccccc gctgcgaatg ctctccgacg caaagacttt gacgacgtcc gcaccttcgc
     8281 cggccagctt gacgaccgcc gcgcgcaacg cctccggcgt cagcttgttg tcgaaaatcg
     8341 gctcgatggc cgtgagaatg cgcggtccgg gcagcgtgcc gcgcgcgatg gcatcacgca
     8401 gcggcttgtc cacgggcgcg ccgagcgact gcacggtggt gatgccgcct tcgagcgtgc
     8461 gcaccgcatt ctcggccgcg taaagcaccg cctcctgcgg cgtctcgttc cgatcggtct
     8521 catcgtcatg cgaccgccca tccttcgcat cgaagtgcca gccgatgtgc acatgcgtgt
     8581 cgacgccgcc cggcatgatc gtgcggtcgc cgaggtcgaa ggtgacgtcc ttcaacgccg
     8641 aggcatgcgg cacgatgcga acgatcttcg ccccgtcgac gaccaccgcc gcatcctcga
     8701 caacatgccc tcgcccatcg atgacgcgcg ccgcacggat gacgatgggc tgggcggcgg
     8761 cggtggtggc gaagaggagg gcgaggagga gggttctcat ggacatagtc tattgaagtg
     8821 tcgtggttgc gcggcttcac agatggacag cgtgcgggct ggcagtccgc acggtgaaat
     8881 cgccggcccg cacggtaaaa cccccggtcc gcgcggtaag acctccggcc cgcaccgtca
     8941 aacctccggc ccgcaccgtc aaacctccgg cccgcaccgt caaaccgccg gcccgcacgg
     9001 ttaaaccaag ggcccgcttg gaaaaccctc gggtccgcgc ggataaacca agggcccgca
     9061 tggaaaaccc tcaggttcgc gcggtaaacc ctcgggtccc gcacacgtct cttcgttttt
     9121 cgttggagtg ccttcccatc gagcgccttc tccccccgcg ctttttgcgg ggggagaagg
     9181 tggcgaagcc ggatgagggg gggcgtaatg cgcagagcga tcaactacac ctcgtgcgaa
     9241 ctgcctgcaa acgcggtaga tgcactgatg tcgtattcat cggaacgccc cctcatccgg
     9301 ccttcggcca ccttctcccc cactgaaaag cgtgggggag aaggcgctcg acgcaaagga
     9361 ggcgctgaaa aggtgtgaga aacgggggtg agtgatgggt tagtgaagaa gcgacctggg
     9421 ttaccgccga cttcatcgtc ccttacgcgc ccaaaacgca acttacgtat acttaattgg
     9481 atgaagccaa gcgacaagac ggcaccagaa cacggcgcgc ggcgcgacct gttcaatgag
     9541 ctgaccgagg gaatggcagc cctttcactc ttgcgcgggc aagggcttcg tcccggcgtc
     9601 aatctggacg acagtgccgc cttgctcgag ttgatggaga gcgatcgtga ttctgatctc
     9661 ctgctctctg acctctgatc tctgatttcc agagacaccg cgccacccac acccgctaca
     9721 ctcatcgcgt gcagattctt gcccgcgcgt accgcggcga tcacgttgag tcgtttcaca
     9781 ccggatccat tgctgtcgtc gactcgctcg ggcgtctcgt cgcctatacg ggcgagccgg
     9841 gccttcggac ctgcctgcgc tcggcggcaa agccgtttca ggcaattcct cttctcgaat
     9901 acggcggcct cgaggagtac gagctctcag gcgaggagct ggcgctcacc tgctcgtcgc
     9961 acggaggcga gccggtgcac gtcgcgaccg cggcggcgct gttgcgcaag ggcgagtttg
    10021 acgaaacaga tctgctctgc ggcgcgcaca tgccgtacga cgagaagtcg gcggcggagt
    10081 tgcgtgcttc cggtgacgag ccctcaccgc ttcacaacaa ctgctccgga aaacatgccg
    10141 ggatgctgct ggcaacgcag gtgatggacg tccccgcggc gcgctacatc gattccgatc
    10201 accctctgca ggagactatg cgcgcgacgc tcagcgattt cgccggtctc gatcccgagg
    10261 agattccgat cgccgtcgac ggctgcggcg tgcctgcgtt ttttctgtcg ctgcaccgcg
    10321 cggcgtttgc ctacgcgcgg ctcatggcca cgagcgcggg ggctgcaccg ggagcgttgc
    10381 agcgctactc cacgagcacg gagcacatcg tcggagcgat gacctcgttt ccttactatg
    10441 tcgcaggcgc ctggagcatc actacgcctc tcatgaaagc attcgacggc gagctgctgg
    10501 cgaaagaagg ggcggagggg ttctacacga tggcacttgc gccctcactc gcatccacat
    10561 tgaccggacg cctcggcctc gcggatgatg tctctctcgg catcgccctg aagatccacg
    10621 atggctcgat ggtgcgcggc cgttatcccg tgattctgag gacgctcgag cttctcggcg
    10681 tcgacatcgc catggccccc gatctgcagc agttccgaaa gctgccggtc gcaaacgtgg
    10741 ccggagcgat cgtcggcgaa gtgcgcgccg aattcgacct ggagatttta tgaaggcaga
    10801 aggcagaaag cagaagacag tagtggtcac aaacagggtt ggcgtgcaga tcggcggagc
    10861 acttctgctc tcggccctct gccttctgcc ttcctgcagc accgtcggcg tctcgaaaac
    10921 gaccggtggc ttcagcgaga tctcgccggc ggtggcggcg gaaatgatca tcgacagtca
    10981 gcagatcgtc gtgatcgacg ttcgctccac ggcagactat cgtggaccgg aagggcacat
    11041 cgccggagcg atcaatgctc cgttcgacgt gatcgagacg cggctgccgg agctgttgcc
    11101 gtaccagaac cagaccgtgc tcgtctatgg tgagacgtcg accgatggcg cggtggcagc
    11161 tcaactgctc accgtcgccg gcttccggaa catcgtccac gtcaccggcg gcatcaagaa
    11221 ttggattgaa cacggctacc gcactgttaa tgcgcagtaa ctgaatccga tccgaggaaa
    11281 aacgaaacta tgaaacgagt gctgatggcc accgcggtgg cacttctccc cttctgtgcg
    11341 caggcgcaaa tggccccttc gctcagtgtc gcgctgaaga acttcgcagt gcgttcgtcg
    11401 gcccaatgtc cggataaccg aatcgcagtc gaccccgtga ccggcgtcaa cggcggcccg
    11461 gccggattct ccctctacga actgacgcag gagtcgtccg atccgagctg ccagcgcaag
    11521 acgtacttgc tcttctctcc agccacgtca caggtcttga tcggaacgat cttcggcctc
    11581 gaacaggacg gtaagacgcc ggctgcgaag atcgttgagg tggcaggcgc agcactcaag
    11641 agcaaagtca ccgcaaccat cgcccccttt cccctgcccg acggcataca cgcggtcgag
    11701 atcacgaagc agtcgaaaga ggccggaggg gccttcaact accacggctt tctcgacgcc
    11761 tcgaacaact ttctgatcgt cggcacccga gggaacctgc gcagcgatcc gggcaaggca
    11821 atgatcgagg cgatcggcct caccggggca atgcgccgtg gcaacccgaa gtcgagagtt
    11881 cagatcatcg agctgtccga cttcgagtgc cccacctgcg cgcgcgcaca caagaaggtc
    11941 gagccgatca tcgcgaagaa cctggacaag gtcgattact accgcctcga cctcccgctc
    12001 ttcgagcatc acgaatgggc cctcagcgcc gccctcggcg cgcgtgcgct cgccaaagtg
    12061 gcaccgtcga agtactggag ctacgtcaat ttcgtgttcg agaatcagga gacgatcggc
    12121 aaacagccct tcgacacttt cctcaagaat ttcgtcgacg ataacgatgt gaactggaaa
    12181 gcggtcgaga agatctaccg ctcccctgcc gagaaaagag cgctcatcga tcaggtcagc
    12241 cgcgctttcg ataacggcat cgtgtcgacg ccgacctaca tcatcaacgg ccagatcctc
    12301 ggcttcggcc ccgagggatc attcacgatc gacgcgatca agaaagcgct cggagtgaag
    12361 tagggcaaac gtttctcacg cgaaggcgcg aagacgcgaa gggctgcgat ggttttcttc
    12421 gcgttctttg cgtgaaagta gtcacgctgg actccacacc gcgaccttgc cggcggcaat
    12481 cgtgaccgtc gcagctgagg cgccggcaac gactcgatat tcgcccggag caagtccgag
    12541 catgccgaag aatccgttgc cgtccgtgcg cgtggttcgc gtgtcgagcg tggccagcga
    12601 ctcgatcgtg acgagcgtgc cgtcgccctc cgcgtaaccc atcaccgccc cgttcgcctc
    12661 ctcggcttgt cgcggcgcgg gtagcgtggc agcgaaaagc ggcaagtcgg tacgttcgaa
    12721 agcagtctgt ccgtcggtgc tcttgcgcag cgccagaaag aactcctcat tcggacgctt
    12781 cggcgtgtaa ataccggcga ccgggatcgc gtacggattg tggcgcacgg cgtcgttggt
    12841 gctgttggcg aggatcgacc acggcttcgt gtcacccatg gcgaagaaga tgattccgtc
    12901 cgcgttcgcg gcacgggcgc ggcgagcctg ccggagcgtt ccttcgatcg agttgagata
    12961 cgcgccgatg cccggcagcg ccgtgcggcg gtttgtgtgc gccgtcgtca cgagaaactg
    13021 cagccagtcg tcgaactgct tcttctccgc cggcacgtgc tcgcgtttgt aaagcatcgg
    13081 gctaccggcg tcgatgatgc cttcgcgcag ccatccttcc caatcctgaa aggcgacgcg
    13141 ataggcgtcc gtgttcttga aaccaccgtt ggcgccgggg ccggtgcccc aggagatgag
    13201 ggccgcggag atcgtgatcg aggggcggat tgccttcgcg ccaagggaca cgcggcggac
    13261 gaaattcgtg acctgctcgc ggcgccagtc gttccagagt ggatccttcg cgcgcggcag
    13321 acgcccgtag cgcgcgcgga acctcgccac gctcgtttcg ttgtatccga cgtcggcatg
    13381 cggcgattcg ggatagcgaa cgcggtcgag gtgcagaccg tcgatgtcgt acgcccgcac
    13441 gaggtgcagc agaacgtcgg ccgtgtacgc cgccgcctgc ggatgcccga ggtcggcgta
    13501 ccagtcggag gagaagagat agcgcccctt gctcgtgcgc gttccccact gccgcgggcc
    13561 acgaaacggc ttgtgcgttt tcgcatccca gaagtgctgc aggaagacat gatgcggatc
    13621 gcggggaacc ggatcgactg ccggatcgcc gcgaaaaacc gctcccacga tcacgaaggc
    13681 gtgcacctga atcccgcggc tgtgcgcgag gtcgagcatc gcgcggagag gatcgaaggt
    13741 gccgtcgagc gtctccgcca gcggctcgga cgcatcgagg taccaggcat cgccgcgccg
    13801 ccgcacctgc acgaaaagcg cgttggcgtt gctgtcgacc gccgcatcga cgatgcgttt
    13861 cacgtcggcc ggagtggcga acggagtacg gaaggtgtcg acccagtacg cgcggtactc
    13921 gtccgcgcac gcgacggagg cagttaggaa gagaacgatt gcgatgaacc gcttcatgga
    13981 tcgtgaagat tgtaaatggt gcgtccagcc cgttttgcca tcattctccg acctcggcgt
    14041 agactcctct tcactcttca cgcttcgttc aacatgcaca tcaacaaacc gccagccggc
    14101 aaccggccac tcaagacgtt ggagcgcgtt ctctcgaagg ccggcctcgg ctcgcgcacg
    14161 gaagcgcgga cgtggatcgg cggcggccgc gtgcaggtga acgggcaagt cgttcgcgat
    14221 cccgacgcct gggtcgacct ggagcttgac cgaatcgaat tcgttggcaa gccgttgcgc
    14281 agcgagcaac gcgtctacgt tcttctgcac aaaccggccg gctacctgac gacctatcgc
    14341 gatccgcagt cccggccgac gatctacgat ctcctccccg accgcgagcg atacctcgtc
    14401 catgtaggcc ggctcgattt cgacacgagc ggtcttctga tcctgacgaa cgacacgcag
    14461 ttcgcggaac ggatgacgaa tccggagtac cacatcccga agacgtatct cgtcgaagcg
    14521 tcgc
//
LOCUS       DATLVW010000492         3998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_65269, whole genome shotgun sequence.
ACCESSION   DATLVW010000492 DATLVW010000000
VERSION     DATLVW010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65269"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VNN25_14865"
     CDS             <1..430
                     /locus_tag="VNN25_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92862.1"
                     /translation="FAGGAFGDACARRFRSGRLWSLAIMTAFFTVPAWMFYSQRPGTQ
                     WFYVCWFLTSAGTTAWFGPLFSAFQELSPVHARSTLVAFALLVLNLLGVGPGPLITGM
                     IGDKHNLSIGLLVSLAVTACAIVPFALAARSEERLVVTRE"
     gene            complement(449..1069)
                     /locus_tag="VNN25_14870"
     CDS             complement(449..1069)
                     /locus_tag="VNN25_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXH92863.1"
                     /translation="MKATTQEERSERSRQLILDAALKLFSHRGYGATSVQDIAVEAGL
                     SKGNVYHHFPDKETIFRSLLDQYFQIMSRPDFPFNRILAEESFPENLERMGFAARDMV
                     REYREYVALIYVDVVEFEGGHVRQFYEHMALRFDAFLKQHDMEQELQSKLANGLSPIS
                     AMMLATRIFMNYFSVEVLFGVKDHFGKGTDEAIREISRILRHGMLK"
     gene            complement(1066..2529)
                     /locus_tag="VNN25_14875"
     CDS             complement(1066..2529)
                     /locus_tag="VNN25_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXH92864.1"
                     /translation="MFAFDLLAERARITPDRLALVSVETGERLTYAQFDHRAALAASA
                     ILETGLQPGDRLGLLAQNSLDFVAFFFGALKAGVIVVPLSTRATTHELSNIAADCAMK
                     VLFHDDASAKVSSELAELNRLKLVSLSARGSAPLAAHHHDPQATACLLYTSGTTGKPK
                     GVMIPHRQLVWNGYNTAVNWGLREDDVSPIFTPLYHAGGLAAFLIPIFCVGGTIVVHR
                     SFDPAEIWATLERERCTVVLGVPTIWKILLEAPESKSAKLGHVRWFASGGAPLPTFLI
                     DAYRERGVTLKQGYGLTEVGVNCFTMTVEDAHTRQGSIGRPVMFTEIRLADENGNEVA
                     TGEIGEMWIRGPHVSNGYWNNPAATAEAYVEGGWFRTGDLARRDSDGFFYIAGRRKDM
                     FISGGVNVYPAEVEAELVQHPGVRDAAVIGVPDPKWGEVGAAFVVANNVGVSDLEAFL
                     AGRLARYKVPRTFVFIDSLPRTPYGKVLKEELRKQLG"
     gene            complement(2508..3326)
                     /locus_tag="VNN25_14880"
     CDS             complement(2508..3326)
                     /locus_tag="VNN25_14880"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH92865.1"
                     /translation="MKDEYLSEVTSQAEYVARARARLAKAGLTRVDLGGTVYWTGGTG
                     PETICLLHGANDHAGSWGWVAPALAKSHRLIIPDLPGHGESDPHTGPIYMQFMVDRVS
                     AILDAESVSRMTLAGNSMGAWVAILHTLAHPEQVSRLVLESGGGLAMPLGVPLVTSNR
                     DEAARMLRAAHGPRAQLPEWTIDALITRSTDSPMVRLVTSDLISHLVDNRLSEIKTPT
                     TIIWGADDGVVPRNYVERLHELITGSELVVIADAAHIPHIQQPERIVECLLSTS"
     gene            complement(3304..>3998)
                     /locus_tag="VNN25_14885"
     CDS             complement(3304..>3998)
                     /locus_tag="VNN25_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HXH92866.1"
                     /translation="GTFDIGCACASFPTALSVANGLMTSNASLRTVLVVGVYQMSKLA
                     RKDDPFLFFYGDGAGAAVLERTDAPGFIAAAFAADGSYHANWLIPAGGTVEPATVESV
                     QSGRTAVTMVQRYPPEINNEGWPRLVRRLAESGGFAIDEIDMIIFTQVRKPTIEIVMR
                     DLGLPLEKTHTIMEQSGYTGSACIPMALHDALQVRKIKPGHLVVLVGSGVGYNQAATA
                     IRMPLHERRIFE"
BASE COUNT          721 a         1222 c         1258 g          797 t
ORIGIN      
        1 ctttgctggc ggcgccttcg gtgatgcatg cgccagacgt tttcgaagcg ggcgtctctg
       61 gagtctggcg atcatgacgg cgtttttcac ggtgccagcg tggatgttct attcgcaacg
      121 gccagggaca cagtggttct acgtctgttg gttcctgacg agcgcgggga ccaccgcctg
      181 gtttggtccg ctgttttcgg cctttcagga actgtccccc gtgcatgcgc gctcgacgct
      241 cgtcgccttc gcgttgctcg tcttgaacct cctgggagtc gggcccggac cgctcattac
      301 cgggatgatt ggggacaagc acaacctctc gatcggtctg ctggtcagcc tcgcggtgac
      361 cgcgtgcgcg atcgttccgt tcgcgctggc cgcgcgcagc gaagagcgac tggtggtcac
      421 gcgtgaatga ccccgtgacg ccgatcgtct acttcagcat cccgtgacga agaatgcgcg
      481 agatctcccg gatcgcctcg tcggtccctt ttccgaagtg atccttgacg ccaaagagaa
      541 cctcgactga gaagtagttc atgaagattc gtgtggccag catcatcgcg gagattggcg
      601 agagtccgtt ggccagcttg gactgcagct cctgctccat atcgtgctgc ttgaggaatg
      661 catcgaagcg aagcgccatg tgctcataga actgccggac gtgcccgcct tcgaactcca
      721 ccacgtcgac gtagatgagc gccacgtatt cgcgatactc acgaaccatg tcgcgcgccg
      781 cgaagcccat gcgctcgagg ttttcgggaa acgattcttc ggccaggatc cgattgaagg
      841 gaaaatccgg tctcgacatg atctggaagt actgatcgag gagtgagcgg aagatcgtct
      901 ctttgtccgg aaagtgatgg tagacgtttc ccttggaaag ccctgcctcg actgcgatgt
      961 cctgcacgct cgtggctccg tacccgcggt gggagaagag cttcagcgct gcatcgagga
     1021 tcaactgccg cgatctctcc gatcgctcct cctgggtcgt tgccttcacc ccagctgttt
     1081 tcgtaactcc tccttcaaca cctttccata cggagtccgc ggcagcgaat cgatgaagac
     1141 aaaggttcgg ggcactttat accgggcaag ccggccggcc aggaacgctt cgagatcgct
     1201 gacgccgacg ttgttggcga caacaaacgc cgctcccact tcgccccatt tcggatcggg
     1261 gacgccgatc actgccgcat cgcggacgcc agggtgttgt acgagctcgg cctccacctc
     1321 ggcaggatag acgttcaccc cgccgctgat gaacatgtcc ttgcgccggc cagcgatgta
     1381 aaagaatccg tcggaatctc tgcgcgccag gtcgccggtg cgaaaccagc cgccctccac
     1441 ataggcctcg gcggtcgcgg ccgggttgtt ccagtagccg ttgctcacgt gggggccgcg
     1501 aatccacatc tcgccgatct ctcccgttgc gacttcgttt ccgttctcgt ctgccagccg
     1561 gatctccgtg aacatgacag gccggccgat cgagccttga cgggtgtgcg catcttcgac
     1621 cgtcatcgtg aaacagttca ctcctacctc ggtcagcccg tatccctgtt tgagcgtaac
     1681 accgcgctcc cggtacgcat caataagaaa cgtggggagc ggggctccgc cgctggcaaa
     1741 ccagcgcaca tggccgagtt tcgcgctctt cgattccggc gcctcgagca ggatcttcca
     1801 gatcgtcggc acgccgagta cgacggtgca ccgttcgcgt tcgagggtcg cccagatctc
     1861 cgctggatcg aaggaacgat gcacgacgat cgttccgccg acgcagaaga tggggatcag
     1921 aaatgccgcc agaccaccgg cgtgatagag gggcgtgaag atcggtgaga cgtcatcttc
     1981 tcggagtccc cagttgactg ccgtgttgta gccgttccag acgagctgac gatggggaat
     2041 catcacgccc ttcggtttgc ccgtcgttcc gctcgtgtac agcaggcagg cggtggcctg
     2101 gggatcgtga tggtgggcgg cgaggggggc ggacccgcgc gcggagagcg aaacaagctt
     2161 caaccgattc agctccgcga gctcgctgct gactttcgct gacgcgtcgt cgtggaacag
     2221 caccttcatc gcgcagtcgg cggcgatgtt cgacaattcg tgcgtcgtcg ccctcgttga
     2281 gagtggcacg acgattacgc cggccttgag cgcaccaaag aagaaggcga cgaagtcgag
     2341 gctgttctgt gcgagcagtc cgaggcggtc gccgggctgg agtcctgtct cgaggattgc
     2401 ggaagcggcg agcgccgccc tgtggtcgaa ctgcgcgtaa gtcaggcgct ctcccgtttc
     2461 gacggagacg agtgccaggc gatcgggcgt gatgcgcgcg cgttccgcta agaggtcgaa
     2521 agcaaacatt cgacgattcg ctccggctgc tggatgtgcg gaatgtgtgc ggcatcggcg
     2581 atcacgacaa gttcggagcc cgttatgagc tcgtgcagcc gttcgacata gttgcgggga
     2641 acgaccccat cgtccgcacc ccagatgatc gtcgtaggtg ttttgatctc cgaaagcctg
     2701 ttgtcgacga gatgagaaat cagatcactg gtcaccagac gaaccattgg cgagtcagtg
     2761 ctccgcgtga tgagtgcgtc gatcgtccat tccgggagtt gggcgcgcgg cccgtgagcc
     2821 gcgcggagca tccgggcggc ctcatcgcga ttcgaagtga cgagcgggac tcccaacggc
     2881 attgccagcc cgcctcctga ctcgaggacg aggcgcgaca cctgctccgg atgcgcgagg
     2941 gtgtggagaa tggccaccca tgcccccatc gaattcccgg caagtgtcat gcgggagacg
     3001 ctctcggcgt cgaggattgc cgacacccgg tccaccatga actgcatgta gatcggtccc
     3061 gtatgagggt cgctctcgcc gtggcctggc aggtcgggaa tgatgaggcg atgtgacttg
     3121 gcgagggccg gtgcgaccca gccccagctt ccggcatgat cgtttgctcc gtgcaggaga
     3181 cagatcgtct ccggaccggt ccctccggtc cagtagaccg tgccgccgag gtccacacga
     3241 gtcaggccgg ctttggccag cctggcccgt gcccgtgcga cgtattcagc ctgcgaagtg
     3301 acttcactca aatattcgtc tttcatggag gggcatccgg atcgcggtcg cagcctggtt
     3361 gtagccgact cccgagccga cgagaacgac gaggtggcca ggcttgattt ttctgacctg
     3421 gagcgcgtcg tggagggcca tcggaatgca cgccgatccg gtgtagcccg actgctccat
     3481 gatcgtatga gtcttttcca acggaaggcc gagatcgcgc atcacgatct cgatcgtcgg
     3541 cttccggacc tgcgtgaaga tgatcatgtc aatttcgtcg atggcgaagc cgccgctttc
     3601 cgcgaggcgg cggaccagcc tgggccaccc ttcgttgttg atctcggggg gatagcgctg
     3661 gaccatcgtc accgccgtgc gacccgactg aaccgactcg acggtggccg gctcgaccgt
     3721 ccctcctgca ggaatcagcc agttggcgtg ataggagcca tcggcagcga acgccgcggc
     3781 aatgaatccg ggagcgtcgg tacgctcgag aaccgcggcg ccggcgccgt cgccatagaa
     3841 gaacaggaag ggatcatcct tgcgcgccag cttgctcatc tggtagacgc cgactaccag
     3901 gactgttcgc agcgacgcgt tgctggtcat caatccgttt gccaccgaga gcgcggtggg
     3961 gaatgatgcg cacgcgcagc cgatatcaaa tgttcccg
//
LOCUS       DATLVW010000493         6875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_230710, whole genome shotgun sequence.
ACCESSION   DATLVW010000493 DATLVW010000000
VERSION     DATLVW010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230710"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VNN25_14890"
     CDS             <1..400
                     /locus_tag="VNN25_14890"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXH92867.1"
                     /translation="PPPTPHPHSFTIRVSARFEAAHFLREYRGISEPLHGHSYKVEVD
                     LASAGGGVDADAIAVDFVSARRKLEALAKTLDYGCINDVPPFDKVNPSAENIAQWFAS
                     SLGAAVAEEKAVVRALTIWEGPVNSVTFRP"
     gene            429..1175
                     /locus_tag="VNN25_14895"
     CDS             429..1175
                     /locus_tag="VNN25_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXH92868.1"
                     /translation="MELTLVIIMLAVGIGLAASVAITFAAAQREADQRGAAQANAAPD
                     RNRVAASILFNLLLSGGIAAETARREIRRGAGLAGPVTSGIDIANWGERFAKIASPEQ
                     RTWLLDTAVRLVADRTTPVPLRQYAALLDLSFSLGFQTDALARLREVYGFDYIDHAKD
                     ARPRGADRAGISTPLYSRDQRETAELLRVLGLAVPADRAGIIMAYRKLAAQHHPDKVF
                     SETADVQSEAAARFIEITRAYEILLSIYSE"
     gene            1595..2365
                     /locus_tag="VNN25_14900"
     CDS             1595..2365
                     /locus_tag="VNN25_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH92869.1"
                     /translation="MKTAVATDHEQGWDFEKAAMPYVDSLYNTAYRMTRNSEDAEDLV
                     QETYLKAYKYYDKFEEGTNFKAWLFKIMKNTFINNYRKKKLTPHKIDFSEIEESYERV
                     IQKNAPDLIKDPESEIFQDMMDADVKRALDSLPHDYKMVVLLADIEGFSYKEIAEILD
                     CPVGTVMSRLYRGRKMLERTLLEYARKYGYIRGTAEPAKMRSRKDETKGRKKKDTETG
                     DEVEIQEPETDEEAILFADDEDIEIEIEEEEPEQFEIE"
     gene            2687..2908
                     /locus_tag="VNN25_14905"
     CDS             2687..2908
                     /locus_tag="VNN25_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92870.1"
                     /translation="MKRVAYFFLDGAMGEKKQTDFKTHVSLCPECEQRVSIHRRLRNF
                     ISRRLGRMAAPPTLRQRLTRSFRALSSDA"
     gene            2924..3481
                     /locus_tag="VNN25_14910"
     CDS             2924..3481
                     /locus_tag="VNN25_14910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HXH92871.1"
                     /translation="MSEHPLILVSSNPNKGIEAERILGVPLLRVSITLPEIQAAGVEE
                     ITRYKLEAARSKGYSHIIVEDVSLGFDELGNFPGPYVKWLLEAAGGKGLAAIAYALNN
                     RAAKAECCVAYWDGTNGEVFRGVTEGEILVEPRGDGNFGWDAWFQPRGSKKTFAEMSP
                     EEKDRVSHRGAAYRQLGAYLKTRNS"
     gene            3657..3836
                     /locus_tag="VNN25_14915"
     CDS             3657..3836
                     /locus_tag="VNN25_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92872.1"
                     /translation="MWIYDGDQWIEEGANETGKQQPETIMPRHGEDFYPELQVVEVEI
                     VPVPQTNYVPHFPLH"
     gene            complement(4031..4546)
                     /locus_tag="VNN25_14920"
     CDS             complement(4031..4546)
                     /locus_tag="VNN25_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92873.1"
                     /translation="MPTLFQKIRLVIRGWQRRRGERDQEFLARSASWSAAPAIAPAAQ
                     AKSRPFTIDTEGLTVAYLDDSGQLAHFLDTESGDIVDVRVADLGASPHLREPRYRRVP
                     SRSEAAEREDRRAFAASLGASPARDVLARCVDAESFRRALATDRTIERAWYNFRNDRA
                     IAAIEAWLETF"
     gene            complement(4644..6728)
                     /locus_tag="VNN25_14925"
     CDS             complement(4644..6728)
                     /locus_tag="VNN25_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92874.1"
                     /translation="MKALVTAILLAAFLSQIPAEAASLSEWRHDIEQIVTDIRTIHPA
                     PFTRTGELTFRRRVAALQKSLPSLSDEQRVVEMMRIVATIGDGHTNLYPDQPSFGLWY
                     PIRVYEFTDGYFVTAAHKSVAELAGAQIIEIAGHPIAEVASQARELVGCDNSFDCEEL
                     LAPLHSAPLMRGLGFAAADGSLAIRARLTNGTTVERILVPHPTDDPRYEKGDSSFDWR
                     FRREVFGTPVGPESDWITAFKNVPASAFRAADPSRPPHLTVARSFFSRPIPESDAFYI
                     QSYAVDENGFAAGFPKALAEVDHLKPRRLIIDLRYNFGGDGSLISPVIHEFIKREAAP
                     PWKELYLLTGRKTFSAGVEAAAMLMKNCELTVVGEPAGAALNSYGDATERDYPHTGLR
                     LYVSSLRHELADSNDLRDTIAVDVPAQFSFAEWSSGRDPAVDPILRGDEMRSIRVIAR
                     AAGGAAARKAYAERQTRFTEMGWWRPPAELELRRVCGQLLAENRVADALDTCVLNSEI
                     HPFVWNTWLNLAQAQRAAGLMLDRLHSLHRVLEIDPTNFNRDEIEKAFEAGLRPPVIR
                     FGATVADIQSAIARSCSALAVREIKPPFLDDVKQKQMQIDCENFPFLGGGRHTEFVFR
                     DDSLEMVWIMTTAAEHESILAAMREAYSDPSIHSAKYDAFPHARAALRVKPAEVLFYS
                     EKVADGVEKEFR"
BASE COUNT         1417 a         2091 c         2098 g         1269 t
ORIGIN      
        1 cccacccccc actccccacc cccactcctt caccatccgc gtctccgccc gattcgaagc
       61 ggcgcacttt cttcgcgaat accgggggat ctccgagccg cttcatggcc actcgtacaa
      121 ggttgaagtt gatctggctt cggccggcgg cggagtcgac gctgacgcga ttgcggtcga
      181 tttcgtcagt gcccggcgaa agctcgaggc actggccaag acgctcgact acggttgcat
      241 caacgacgtt cctccgttcg acaaggtcaa tccgtctgcc gagaacatcg cgcaatggtt
      301 tgcaagctcc ctcggggctg ccgtggccga ggagaaggca gtggtccgcg cgttgacaat
      361 ctgggaaggg ccggtgaatt cggtcacctt ccggccttga cgcctcggca cgcgtaccat
      421 tccgttccgt ggagctgaca ctcgtcatca tcatgctagc cgtcggcatc gggctggcgg
      481 cgtccgtggc tatcacgttc gctgcggcgc aacgggaagc cgaccagcgc ggcgcggcgc
      541 aggcgaacgc tgctcctgac cgcaatcgag tagcggcatc gatccttttc aaccttctgc
      601 tttccggcgg catcgcggcg gagactgccc ggcgcgagat ccggcgtggt gccggcctgg
      661 ccggaccggt cacctcggga atcgacatcg ccaactgggg tgagcgcttc gcgaagatcg
      721 cctcgcccga acaacggacg tggctgctcg acaccgccgt gcgcctcgtc gccgaccgca
      781 ccacgccggt gcccttgcgg cagtacgccg cgctgctcga tctcagcttt tccctcggct
      841 tccagacaga cgccctggcc cggctgcgcg aggtttacgg cttcgattac atcgaccatg
      901 cgaaagatgc gcggccacgc ggagccgacc gcgcggggat ctcgacgccg ctctactcgc
      961 gtgatcaacg cgagaccgcc gagctgctac gagtgcttgg actcgccgtg cctgcggatc
     1021 gtgccggcat catcatggcc tatcgcaagc tggcggcgca gcatcacccg gacaaggttt
     1081 tcagcgaaac cgcggacgtc cagagcgagg cggcggcgcg cttcatcgag atcacgcgcg
     1141 cgtatgagat acttctcagc atctactctg agtgactgga acctcagaac gcttcctgtc
     1201 acgagagggt gtcggatcgg cctcgaacgt acgtagggct tgcgtacgat tgtcagccgt
     1261 gtataatgcc cgtccgggaa ttcgggcagt ttcctgtttg ttcacagccg gtttgccagc
     1321 acttccctcc gttagaccgt agtaaatctc gacgacagca acaatcagga cggacgtgtc
     1381 cggtcgcggt gacgcggcac cgaccagtcc gaatgtcgaa gaggtcgtag agacaatcgc
     1441 catccgaacg tcgtaaccca taccgttaca acgacttcgg atagctcact ccagcaaatc
     1501 tggggccctc ggtaactatt tttttcgccg cgcatctaat aaggataacg gcacgagtta
     1561 cgcatttttg gatgtttgcc gtacaggaga cacgatgaag accgcagtag caaccgatca
     1621 cgagcaggga tgggacttcg agaaggccgc gatgccctac gtcgattccc tgtacaacac
     1681 cgcctatcgc atgacgcgga actccgaaga tgcggaggac ctcgttcagg aaacctacct
     1741 caaggcgtac aagtactacg acaagttcga ggaaggcacg aacttcaagg cgtggctctt
     1801 caagatcatg aagaacacgt tcatcaacaa ctatcggaag aagaagctca cgccgcacaa
     1861 gatcgacttc tccgagattg aagagtcgta cgaacgggtc atccagaaga acgcgccgga
     1921 tctgatcaag gatccggaga gcgagatctt ccaggacatg atggacgctg acgtcaagcg
     1981 tgccctcgat tcgctgccgc acgactacaa gatggtcgtt ctgctcgccg acatcgaagg
     2041 cttctcgtac aaggagatcg ccgagatcct cgactgcccg gtcgggaccg tcatgtcgcg
     2101 cctgtaccgt ggccgcaaga tgctcgagcg cacgctgctc gagtacgcgc gcaagtacgg
     2161 ctacatccgc ggcactgcgg agccggccaa gatgcgctcg cgcaaagacg agacgaaggg
     2221 ccgcaagaag aaggacaccg agaccggcga cgaggtcgag attcaggagc ccgagactga
     2281 cgaagaggcg attctgttcg ccgacgacga ggacatcgag atcgagatcg aagaggaaga
     2341 accggagcag ttcgagatcg agtagctcca gcgttcagca acaatcaagt cttcgggcgg
     2401 ccggttgttt tcggccgccc gaagtgtttg ttacagagat cagagacgag agattagaga
     2461 tcagggttga acgcgctctc tttctgtctt tgatctctga tctccccgtt ttgccagaga
     2521 caagagatca gagatccgct tcctgacggc ctcggctgtc tctgatctct gttgtctctt
     2581 atctctgatt catcaccgga attcagcctg aggcacggca gttgcgaccg atgcagagtc
     2641 gtcacctcga tggccaagga gaccgcagtc aatgatctgt gacgatgtga aacgagttgc
     2701 ttacttcttc ctcgatggcg caatgggcga gaagaaacag accgacttca agactcacgt
     2761 cagcctctgt cccgaatgcg aacagcgcgt cagcatccac cggcgtctga gaaacttcat
     2821 cagtcgaagg ctcggacgca tggcagcgcc gccgactctc cggcagcgcc tcacccgcag
     2881 cttccgcgcc ttgagcagcg acgcctagac atagaatcca agcgtgtctg agcatcccct
     2941 gatcctcgtt tcttcgaatc cgaacaaagg aatcgaagcc gagcgaatcc tcggagtccc
     3001 tttacttcgc gtctcgatca cgctgcccga gattcaggcc gccggcgttg aggagatcac
     3061 gcgctacaag ctcgaggccg cccgttccaa gggctacagc cacatcatcg tcgaagatgt
     3121 ttccctcggc tttgatgagc tgggcaactt tccggggccg tatgtgaagt ggctgttgga
     3181 ggccgcaggc ggaaaaggcc tcgctgcgat cgcgtacgcg ctcaacaacc gcgcggcaaa
     3241 ggcggaatgc tgtgtcgcct actgggacgg cacgaacggc gaagtcttcc gcggcgtgac
     3301 cgagggcgag attctcgtcg agccgcgggg cgacggcaac ttcggctggg atgcctggtt
     3361 tcagccgcgg ggatcgaaaa agaccttcgc tgagatgtca cccgaggaga aggatagggt
     3421 ctcgcaccgc ggtgcggcgt accgccagct cggcgcgtat ctcaaaacgc gaaactcatg
     3481 acccgggaac cggacgcact gagtccgcgc gaggacggcg cctcaaacca tctgccggga
     3541 tcatccgtag aagacattga gaatgagtga tttggccgtg ccacccattg gcaggccaat
     3601 tgaagtatgc aggatagcct tcggagactc cgagggagca gtggaaagga cgcccaatgt
     3661 ggatatacga cggtgatcaa tggatcgaag agggcgcgaa cgagaccggg aagcagcagc
     3721 cggagacgat catgccccga cacggcgaag atttctaccc cgagttgcag gtcgtcgaag
     3781 tcgagatcgt cccggttccg caaacgaact acgtcccgca ctttccgctg cattaacgaa
     3841 acagaactcc gttaacgacc cgccgcgcct tcgggcgcgg ctttttgttt gaggcagaga
     3901 tcagagacta gagatcagag accaggaggc agagatcagg aaacagagat caggaaacag
     3961 agatcagaga ccaaggagtc gacgctgccg acgtgtcccg atctctgatc tcttgtccct
     4021 gatctctgct tcagaacgtc tccagccacg cctcgatcgc tgcgatggcg cggtcgttcc
     4081 tgaagttgta ccaggcgcgc tcgatcgttc ggtccgtggc cagagcgcgg cggaacgatt
     4141 ccgcatcgac gcagcgcgcc agcacatcgc gggcgggcga tgcccccagc gacgcggcga
     4201 acgcacggcg atcctcacgc tccgcggcct cgctgcgcga aggcacgcgt cggtaacgcg
     4261 gctcccgaag gtgcgggctc gcgccgaggt cggccacacg aacgtcgacg atatctccgc
     4321 tctcggtgtc gagaaaatga gcgagctgcc ccgagtcgtc gagataggcg acggtcagcc
     4381 cttcggtgtc gatcgtgaag gggcgggact tcgcctgcgc tgctggagca atggccggcg
     4441 cggcgctcca cgacgcactc cgcgcaagaa actcctgatc gcgctcgccg cgccgccgtt
     4501 gccagccgcg gatcacgagg cggatcttct ggaagagggt tggcattcct gtcggatcag
     4561 tctagcgcgc cttcgtgctg gtcaggccct cgccaaaagg cacaggggct ctgcgtttct
     4621 gtggttccgc agtcgcgaag gatctagcgg aattccttct ccacgccgtc tgcaaccttc
     4681 tcagaataga acagcacctc ggcgggcttg acgcgcagtg cggcccgcgc gtgggggaaa
     4741 gcgtcgtact tcgcactgtg gatcgacggg tcgctgtagg cctcgcgcat cgctgcgaga
     4801 atcgattcat gttccgccgc ggtggtcatg atccaaacca tctccagcga gtcatcacgg
     4861 aaaacgaatt ctgtgtgacg cccgccgccg aggaatggaa aattttcgca gtcgatctgc
     4921 atctgtttct gctttacgtc gtcgaggaac ggcggcttga tctcgcgaac ggcaagcgcg
     4981 gagcacgacc tggcaatcgc ggattggatg tcggcaaccg ttgcgccgaa acggatcacg
     5041 ggtgggcgaa gcccggcctc gaatgccttc tcgatctcat cgcggttgaa gttcgtcggg
     5101 tcgatctcga gcacgcggtg gaggctgtgc aaacgatcga gcatcagtcc cgcggcacgc
     5161 tgcgcctggg caagattgag ccacgtgttc cagacaaaag gatggatctc gctgttcagc
     5221 acgcaggtgt cgagggcgtc cgccacacga ttctccgcga ggagctgccc gcaaacccga
     5281 cgcaactcca actccgccgg cggtctccac caacccatct cagtaaagcg cgtttgccgt
     5341 tccgcatacg ccttcctcgc tgcagctccg ccagcggcgc gcgcgatcac gcggatgctc
     5401 cgcatttcat cgccgcgcag aatggggtcg acggccggat cgcggccgct ggaccattcc
     5461 gcgaacgaga attgcgccgg aacgtcgacg gcgattgtgt cgcgaagatc gtttgagtct
     5521 gccagttcat gccggagcga cgatacgtag aggcgaagac cggtgtgtgg gtagtcccgc
     5581 tccgtggcgt cgccgtagga gttgagcgcg gcgccggcag gctcaccgac gacggtgagc
     5641 tcgcagttct tcatcagcat agccgccgcc tcgacacccg cgctgaatgt cttgcgtccg
     5701 gtcaaaaggt agagctcttt ccagggaggt gccgcttccc gtttgatgaa ttcgtgaatg
     5761 accggagaaa tgagactgcc gtcgccgccg aagttgtagc gaagatcgat gatcaggcgt
     5821 cgcggtttca ggtgatcgac ctcggcgagc gccttcggaa acccagcggc gaagccgttc
     5881 tcgtcgacgg cgtacgattg aatgtagaac gcatcgctct ctggaatcgg cctgctgaag
     5941 aagctccgcg cgaccgtcaa gtgcggaggc cgcgaaggat cagcagccct gaaggcgctt
     6001 gcgggcacat tcttgaacgc cgtgatccag tcgctctcgg gcccgaccgg agtaccgaaa
     6061 acctcgcggc gaaaacgcca gtcgaaactg gagtcacctt tctcgtatcg aggatcgtct
     6121 gttgggtgcg gcaccaggat gcgctcgact gtcgtcccgt ttgtcagcct cgcgcgaatc
     6181 gcaagcgatc cgtcagccgc ggcaaaaccc aatccccgca tcagcggagc gctgtgaaga
     6241 ggtgcgagca gttcttcgca gtcgaaggag ttgtcgcagc caacgagctc gcgcgcctga
     6301 gacgcgactt cggcaatggg atgtccggcg atctcgatga tctgggcgcc ggcaagttcg
     6361 gcgacgctct tgtgcgccgc ggtaacgaaa tagccgtccg tgaattcgta gacgcgaatg
     6421 gggtaccaga gcccgaacga gggctgatcg ggatagaggt tcgtatgtcc atccccgatc
     6481 gtcgccacga tgcgcatcat ctccacgacg cgctgctcgt ccgaaagcga aggaagggat
     6541 ttctggagcg ctgcgacacg gcggcgaaac gtcagctctc ctgtcctggt gaatggtgcc
     6601 ggatggatcg tccggatatc ggtcacgatc tgttcgatgt catggcgcca ttcagaaagc
     6661 gacgcggctt ccgcggggat ctgtgacaga aatgccgcaa gcagaatggc ggtgactaac
     6721 gctttcatcc aggtctcctc cctacaagca acaaatgcgt cgaaggagag atgtatcgcc
     6781 aaacgtgccg cagcgtcttg tgcaaaagaa gcacgtcagc gcgccagtcg ccggtacaac
     6841 cctggcgcga ctccccaggc gcggcgaaag tgctt
//
LOCUS       DATLVW010000494         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_391471, whole genome shotgun sequence.
ACCESSION   DATLVW010000494 DATLVW010000000
VERSION     DATLVW010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391471"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1483)
                     /locus_tag="VNN25_14930"
     CDS             complement(77..1483)
                     /locus_tag="VNN25_14930"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HXH92875.1"
                     /translation="MIEKKHRTLSSIYLINDALASNLAMLCAWFLRFSVKIIPVTKGQ
                     QEVETYLQLLPIVTIVFPLAFAVQGLYRIRPARSKTEEWLSVAIGSIVATVVFSGVLL
                     WVRPGRPDILYSRATLLIFLVCAIVLVTLGRGIVRAVVERGYRKGKRLDRVLIAGSGE
                     LARAVVERVRGHRELGFTLVGYLRNGEQTALEGLECLGTIDDAEHVIAERKIDHVFVA
                     LPHASSEAMMALLDRLVRSVVAIHVVPDLLQFMVLRSRVEDLDGLPTINLSETPLEGW
                     SRFLKRGFDLIVALAAFIVFSPVMLLVAIIIKLEDRGPVFYRQERMGLDGKPFDIFKF
                     RSMRVGAENESGAKWAEKDDPRRTRVGRFIRKTSIDELPQLLNVLLGDMSVVGPRPER
                     PQFVEQFRAEFPHYMLRHKVRAGMTGWAQVHGWRGNTSIRIRIEHDLYYIENWSLMLD
                     LKILFMTLVHGLRHENAY"
     gene            complement(1480..2688)
                     /locus_tag="VNN25_14935"
     CDS             complement(1480..2688)
                     /locus_tag="VNN25_14935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92876.1"
                     /translation="MNSADAGSSFIPQPSSFGRVAVIHDWLTGMRGGENVLEAILDLF
                     PDAELFTLFHFPGTVSDAIEKHTIHTSRLQALASRVSDYRTLLPLFPRAVRQWDLSGF
                     DLVISSSHAVAKGVDSRGKPHLCYCHTPMRYIWDRFDDYFPRGVKRLLARPLAARLRR
                     WDVANSKHVTRFVANSNFVRERIREYYGRDAAVVHPFVDDAFLSVPLRESRDDFHVVL
                     SALVPYKRVELAIEAAEASGRRLVVIGGGPLLPNLRRRSGPNVQFLGPVSREVIIDHL
                     GRAQSLILPGVEDFGITPLEAMALGTPVVALGTGGVRDSVVPGTTGIFFETASVDSLR
                     GALSEAERRDWDRAAIRAHAATFSRERFQREMLAELEQVVGTGVGSRGSERARNSDSR
                     LPTPHSRNSP"
BASE COUNT          540 a          896 c          890 g          487 t
ORIGIN      
        1 ctcccgactc cctaatctct gatctctgct ccctgatctc tgctccctga tctctgtttc
       61 ctgatctctg ctccactcag tacgcgttct cgtgacgcaa cccatgcacc agcgtcatga
      121 acagaatctt caggtcgagc atgagtgacc agttctcgat gtagtagaga tcgtgttcga
      181 tgcggatgcg gatcgatgtg ttgccgcgcc atccgtggac ttgcgcccat ccggtcatgc
      241 cggcgcgcac tttgtggcgg agcatgtagt gcgggaactc cgcgcggaac tgctcgacga
      301 actgcggccg ctccgggcgc gggccgacga cgctcatgtc gccgagtagc acgttgagga
      361 gctgcggcaa ctcgtcgatc gacgtcttgc ggatgaagcg tcccacgcgc gtgcgccgcg
      421 gatcgtcttt ctctgcccac ttcgcgccgc tctcgttctc ggcgccgacg cgcatcgagc
      481 ggaacttgaa aatatcaaac ggcttgccgt cgagtcccat ccgctcctgc cggtaaaaca
      541 ccggcccgcg atcctcgagc ttgatgatga tggccacgag cagcatgacc ggcgagaaga
      601 cgatgaatgc cgcaagcgcc acgatcagat cgaagccacg tttcagaaag cggctccaac
      661 cttcgagcgg cgtttcggaa aggttgatcg tcggcagccc atcgaggtcc tcgacgcgcg
      721 accggagcac catgaactgg agcagatccg gcacgacgtg gatggcgacc acgctccgca
      781 ccagccggtc cagcagcgcc atcatcgcct cggacgacgc atgcggcagc gcaacgaaga
      841 catggtcgat ctttcgttcc gcgatcacgt gctccgcgtc gtcgatcgtg ccgaggcact
      901 ccaaaccttc cagcgccgtc tgctcgccgt tgcgaaggta gccgacgagg gtgaagccga
      961 gctcgcgatg tcccctcacc cgctcaacca ccgcgcgggc cagctcgccg ctgccggcga
     1021 tcagcacgcg gtcgaggcgt ttgcccttcc ggtagccgcg ctcgaccacc gcccgcacga
     1081 tgccgcggcc gagagtcacc agcacgatgg cgcagacgag aaagatcagc agcgtggctc
     1141 gcgaatagag aatgtccggc cttcccggcc gcacccagag cagcacgccc gagaacacga
     1201 cggttgccac gatcgagccg atcgccacag agagccactc ttcggtcttc gagcgcgccg
     1261 gccggatgcg atagaggccc tggacagcga aggcgagcgg aaaaacgatt gtgacgatcg
     1321 gcaggagctg gagataggtc tccacctcct gctgcccttt cgtgaccgga atgatcttga
     1381 cgctgaagcg gaggaaccag gcgcagagca tggccaggtt cgacgccagg gcgtcgttga
     1441 tcagatagat cgacgaaagc gtccggtgtt ttttctcgat cacggcgaat tccgggagtg
     1501 gggagtcggg agtcgggagt cggagtttct tgcgcgctcc gacccccgac tcccgactcc
     1561 cgttccaacg acttgttcca actccgcgag catctcacgc tgaaaccgtt cgcgcgagaa
     1621 ggtcgccgcg tgggcgcgga tggccgcacg gtcccagtcg cgacgctccg cttcgctcag
     1681 cgctccgcga agcgagtcta ccgatgcggt ctcgaaaaaa atcccggttg taccggggac
     1741 caccgagtcg cgcacgccgc ccgtgccgag agcgacgaca ggcgtgccta gggccatggc
     1801 ctccaacgga gtgatgccga agtcttcgac accgggaaga atgaggctct gcgcgcgccc
     1861 gaggtggtcg atgatcacct ctctcgatac gggcccgagg aactgaacgt tggggccgct
     1921 tcgccggcga aggttcggga ggagcggacc gccgccgatc acgacgagtc ttctgccact
     1981 ggcctccgcg gcctcgatgg ccagctcgac gcgcttgtag ggaacgagcg cggagagcac
     2041 gacgtgaaaa tcatcgcgcg attcgcgcag cggcacggac aggaacgcgt cgtcgacgaa
     2101 cggatgcacg accgctgcgt cgcggccgta gtactcgcga atgcgttcgc gaacgaaatt
     2161 cgagttggcg acgaatcgcg tgacgtgctt cgagttggca acatcccacc ggcgaagacg
     2221 tgccgcgagc ggacgcgcca gaagccgttt cacgccgcgc ggaaagtagt catcgaagcg
     2281 gtcccagatg tagcgcatcg gtgtgtggca atagcaaagg tgcggcttgc cgcggctgtc
     2341 gacacctttg gcgacggcgt gactggagct gatgacgaga tcgaatcccg atagatccca
     2401 ctgccgcacg gcccgcggga aaagcggcag cagcgtgcgg tagtcgctga cgcgcgaggc
     2461 cagcgcttgc agccgcgacg tgtgaatcgt gtgcttttcg atcgcatcgg agaccgttcc
     2521 cggaaagtga aaaagcgtga agagctccgc gtccgggaag aggtcgagga tggcttcgag
     2581 gacgttctcg ccgcctcgca tgccggtcag ccagtcgtgg atgacggcga cacggccaaa
     2641 ggatgaaggt tgagggatga aggatgaacc ggcgtcagcg ctgttcatcg ttcatccctc
     2701 atccttcatc cttcgtttcc cgcggctcgg gcgtcgatgc cagcggatag tcgccgtccg
     2761 ccgcgcaatc gtcgcagagg tgatcgttgc gcagatactc ttcaagctca acc
//
LOCUS       DATLVW010000495         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_355574, whole genome shotgun sequence.
ACCESSION   DATLVW010000495 DATLVW010000000
VERSION     DATLVW010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355574"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="VNN25_14940"
     CDS             complement(<1..1902)
                     /locus_tag="VNN25_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92877.1"
                     /translation="MPVNRMPGHLSKNWATLLLVILALTALVPNAAAQTPPYWNSGNP
                     QLANGAPGSAIKPAPWPTELPSQWVPYSWGTTFPDATIVDWHPIRDPRVQDPSNGGTS
                     PQNYVNVSSGCGDKTLPSIYYYFDKNADSGAGMIYFRWRVEQIANSYATGPSAGAFSS
                     SDPWKSALWTVFFDTNGDGFRDFAAHLDGSSGSPAIPVDVLRSIWSATKTNSIDYVTG
                     GTGIYSLFSNPTAFVDNTTQSILQFNGATSPTLPVPSTVQWPNGASETIWDYGTTRSI
                     NLTSGSCVEYYVDYEIPLRMLNASGAGVNGGVTTNPLGGPQFKSDTPFQFLFATANSL
                     NNPFQKDIVWEGNFVCDATSPGPFGDALTLDGGLIPQPIATAITAGAQNSCAVPVSAQ
                     IMDALTVNNCQSISQLVNAQFKYWYDTNGDGLPNDVGSSWVNIGDPTVPVGTTVNANW
                     NLNNLIQGQYLIALEITDNRGHTTQTWRCSSLTDATDLSDNAAQQCRTVVSTPTTSAT
                     LGVDGLGRHIYPNAPYGGLFTAAGGTSTFTHPKTLGVNYAKVTIGGTCGAPPPTVTKT
                     HNVGSVAAGGAITYTLTINNTSSTAVSVLSVSDTLPAGFTSPSAGGGTLCTGGCTISG
                     PTLVGGAEQW"
     gene            complement(1988..2386)
                     /locus_tag="VNN25_14945"
     CDS             complement(1988..2386)
                     /locus_tag="VNN25_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HXH92878.1"
                     /translation="MSLFEATLRDLLASGHRARFRAMGDSMYPAIRNGDFVEIAPCQV
                     SDLRRGDIVLASIERGLTLHRVVRIAPDGIVMRGDNALTTDPPFGPSDLLGRAICEEI
                     TQDSRPFDALVKIIRNAARFRRRLQYRFRH"
     gene            complement(2383..>2995)
                     /locus_tag="VNN25_14950"
     CDS             complement(2383..>2995)
                     /locus_tag="VNN25_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92879.1"
                     /translation="FGAAPYKTALVDSDFRHGRILVREVSSEAMHPLEYPLDEVLIAR
                     LLGRGRGVELHGCGVIDRDGRGRLFVGQSGAGKTTTARVWLADGDYEIVSDDRVIVRF
                     IDGEWRMYGTPWHGEAELSSPLSAPLSAIHLLVQASQTELVALSSAQAAASLFGCTFP
                     PFYDAAAIEFTLDSLARIVADVPVRALRFVPDRSLIDCVRSAA"
BASE COUNT          546 a          889 c          981 g          579 t
ORIGIN      
        1 ccactgttca gctcctccca caagggttgg tcccgagatc gtgcagccac ccgtgcagag
       61 tgtgccgccg ccggcgcttg gagaggtaaa ccctgctgga agtgtgtcgc tgaccgacag
      121 aacgctgacc gccgtgcttg atgtgttgtt gatcgtcagc gtgtaggtga tcgcaccgcc
      181 ggcggccacg ctgccgacgt tatgggtctt ggtcactgtc ggcggcggcg cgccgcaggt
      241 tcccccgatg gtcaccttcg cgtagttgac gccaagggtt ttcggatggg tgaaggtact
      301 cgtgccgccg gcagccgtga aaaggccgcc gtagggcgcg ttcggataga tgtggcggcc
      361 gaggccgtcg acgccgagcg tcgcgctcgt cgtcggagtg ctcaccaccg ttcggcattg
      421 ctgggcggcg ttgtcactca ggtcggtggc gtcggtgagg ctactgcagc gccaggtctg
      481 cgtcgtgtgc ccgcggttgt cggtgatctc gagtgcgatc aggtactggc cctgaatcag
      541 gttgttcaga ttccagtttg cgttgaccgt cgttccgacc ggcactgtcg gatcgccgat
      601 attgacccaa ctgctgccca catcgttagg aaggccgtcg ccgttcgtgt cataccagta
      661 cttgaactgg gcgttgacga gctggctgat cgactgacag ttgttgaccg tcagcgcgtc
      721 catgatctgg gcgctgactg ggacggcgca actgttctgc gctcctgccg tgatcgcggt
      781 ggcgatcggc tgcggaatga gtccgccgtc gagtgtcagc gcgtctccga aggggccggg
      841 cgaggtcgca tcgcagacga agttcccctc ccacacgatg tccttctgga agggattgtt
      901 cagactattc gccgtggcga aaagaaactg gaagggtgta tcggacttga actgaggtcc
      961 gcccagagga ttcgtcgtga cgccgccatt cacaccggcg cccgacgcgt tgagcatgcg
     1021 caacgggatc tcgtaatcga cgtagtactc gacgcacgaa ccgctggtga ggttgatcga
     1081 gcgcgtcgtg ccatagtccc agatcgtctc gctggcacca ttcggccact ggaccgtgga
     1141 cggcacgggc agcgttggcg aggttgcgcc gttgaattgc aggattgatt gcgtcgtgtt
     1201 gtcgacgaat gccgtagggt tcgaaaagag gctgtagatg cccgttccgc ccgtcacgta
     1261 gtcgatcgag ttcgtcttcg ttgcgctcca gatcgatcgg agcacgtcaa cgggaatcgc
     1321 cggcgatccg ctcgagccat cgagatgagc ggcaaaatcg cggaagccat cgccattcgt
     1381 gtcgaaaaac accgtccaga gagcggactt ccagggatcg ctgctgctga acgcgcccgc
     1441 actcgggccc gttgcatagc tgttggcgat ctgttcgacg cgccaacgga agtagatcat
     1501 tccggcgccg ctgtcggcgt tcttgtcgaa gtagtagtag atgctgggca gcgtcttatc
     1561 gccgcatcct gagctgacgt tgacgtagtt ctgaggagac gtgccgccgt tggacggatc
     1621 ctgaacgcgt ggatcgcgga ttggatgcca gtcgacgatc gtggcatcag ggaaggtcgt
     1681 tccccaggag tagggaaccc actgactggg cagttcggtc ggccatggcg ctggtttgat
     1741 cgccgaaccg ggcgcaccgt tcgcgagttg cggattgccg ctgttccagt agggcggggt
     1801 ctgcgcggcc gcgttcggca cgagagccgt cagggccagg atgaccagca gaagcgtggc
     1861 ccaattcttc gacaaatgcc ccggcatgcg attgactggc atgaacaaac tcctgacgcc
     1921 gcgcgagccc gtggcacgcc ggcatccgct ctcaaatgtg gtttggacct tggctatctt
     1981 actgctttca gtggcgaaaa cgatactgca gacgacgtcg aaacctcgcc gcattgcgta
     2041 ttattttcac taacgcgtca aaaggccgcg aatcctgcgt gatttcttcg cagatcgcgc
     2101 gtccgaggag gtcggagggt ccgaatggcg ggtccgtggt cagagcgtta tcgccgcgca
     2161 tgacgatgcc atccggcgcg atgcgaacga cccgatggag cgtcagtcca cgctcaatgg
     2221 atgccagcac gatgtcgccc cggcgcagat cggacacctg gcaaggtgcg atctcgacga
     2281 aatcgccgtt ccgaattgcc ggatacatgc tgtcgcccat ggcccggaat cgggctcgat
     2341 gtcccgacgc cagcagatcg cggagcgttg cttcaaaaag actcacgccg cgctccgaac
     2401 acagtcgatg agactccggt ccggtacgaa cctcagagcg cgtaccggca catcggcgac
     2461 gatccgcgcc agcgagtcca gcgtgaattc gatcgctgcc gcgtcataga aaggcggaaa
     2521 ggtgcagccg aaaagagaag ccgcggcttg cgcggaggag agtgcgacca gctccgtttg
     2581 cgatgcctgc acgagcagat gaatcgccga taacggagcg gacaagggag aggaaagctc
     2641 ggcctcgcca tgccagggcg tgccgtacat ccgccattcg ccgtcgatga atcggacgat
     2701 gacgcgatcg tcgctgacga tctcatagtc gccatcggcg agccagaccc gcgccgtggt
     2761 cgtctttccc gcccccgact gtccgacgaa aagccgccct cgcccatccc ggtcgatcac
     2821 gccgcagccg tgcagttcga cgccgcggcc tcgcccgagg agtcgcgcga tcagcacctc
     2881 gtcgagagga tattcgagag gatgcatcgc ctcgctggag acctcgcgca cgaggatccg
     2941 gccgtggcgg aaatcgctgt cgacgagtgc ggttttgtag ggggcggcgc cgaag
//
LOCUS       DATLVW010000496         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_270570, whole genome shotgun sequence.
ACCESSION   DATLVW010000496 DATLVW010000000
VERSION     DATLVW010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270570"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..568
                     /locus_tag="VNN25_14955"
     CDS             71..568
                     /locus_tag="VNN25_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92880.1"
                     /translation="MSRNPLLIVPIRDRRQRRRILTIKNCALTMLSLAVVFASISIYS
                     ASRRGKTADYGRLFGTQVGAPNSGVSRNLEVVHEGTVADQSAPDPMLVAPAGREQLLM
                     AKSNVDPTPAPTSQVTPAPITVVPASAKAHETTIVGDNNGIVVAKPASTTTAHPVLSG
                     GIFKQ"
     gene            658..2121
                     /locus_tag="VNN25_14960"
     CDS             658..2121
                     /locus_tag="VNN25_14960"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HXH92881.1"
                     /translation="MSQPITFGTDGWRAVIADGFTFDNVRRVSDAIAVAARTLEPPDG
                     IDRNALVVGFDRRFLSREFATLVAETLRDGGFRVVLSTAPTPSQTISFTAWRRKLLGG
                     VVVTASHNPAKYNGLKFKAWYGGSALPEMYAAIAGALGQSSKRDGGSIEESDLLDDYI
                     AALRAQLDVRKMASLRILHDPIHGVASGLPTRILGIDVGTIRGENNPSFGGVNPEPIP
                     DNLAASMRESGVGSRVSESSPSESASAPTPDPRQPTPAKYDLAICNDGDADRLGILDE
                     RGVFVSPQKIIALLALYLVREKRMTGEIVKTFSTSRLVETVARKLGVPFHETAIGFKY
                     VADLMLTRNILIGGEESGGIGFGHFLPERDGILSGLLVAECIAHFGKPLSHLIDAMET
                     EFGALHYDRRDLNRPMEVCARLIERVRAGELNAVYGGTFASLEEKDGVKLNFTDGSWI
                     LFRKSGTEPIIRIYCESPDAGQVAAMLDVAIAELDRS"
     gene            complement(2193..>2598)
                     /locus_tag="VNN25_14965"
     CDS             complement(2193..>2598)
                     /locus_tag="VNN25_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92882.1"
                     /translation="SRWLLAFSSFLFISLALAKRYADLTRAVSNDEEAPGGRSYRTGD
                     REVLLSLGTSSGLISVLVFALYLNSAQVTQLYSKTDPLWLICALLMYWIGRMWLLAHR
                     GEIDDDPIVTAATDPASYIVAAAAALTVLASI"
BASE COUNT          505 a          896 c          743 g          454 t
ORIGIN      
        1 cgacccccga ctcccgacac cctacccccg caacccacac tggcagcccc ctcgcattga
       61 ttcaccacaa atgtccagaa acccgctgct catcgtgccg attcgcgatc ggaggcaacg
      121 ccgccgcatc ctcacgatca agaactgtgc gctgacgatg ctgagtcttg cggtcgtgtt
      181 cgcctcgatc tcgatctact cggcgtcgcg gcggggtaag acagccgact atggccggct
      241 cttcggtacg caggtcggcg cgccaaacag cggcgtctcc cgcaacctcg aggtcgttca
      301 tgaaggcacg gttgccgatc agtccgcccc cgatcccatg ctcgtcgctc ctgccggccg
      361 cgagcagttg ttgatggcga agagcaacgt cgatccgact ccagcgccca ccagccaggt
      421 cacccccgcg ccaatcaccg tcgtccccgc gagtgcgaaa gcgcacgaaa cgacgatcgt
      481 cggcgataac aacggcatcg tcgtcgcgaa gccggcatcg accacgaccg cgcatcccgt
      541 cctttccggc ggaatcttca agcagtgatc cgcagatgtc gcagatggaa cgcagatttg
      601 agactgcctt atctgcgttc atctgcgaca tctgcggata gcctctagaa tgcgccgatg
      661 tcgcaaccaa tcacattcgg caccgacggc tggcgcgcgg tcatcgcgga cggcttcacc
      721 ttcgacaacg tgcgccgcgt cagtgatgca atcgccgtcg cggcgcgcac gctcgaacca
      781 cccgacggga tcgatcgcaa cgcgctggtc gtcggattcg accgccgctt tctttcgcgc
      841 gaattcgcta cgctcgtcgc ggagacgttg cgcgacggcg gcttccgcgt cgttctgtcg
      901 accgccccaa ccccctcgca gaccatctcc ttcaccgcct ggcgccgcaa gctcctcggc
      961 ggcgtcgttg tcaccgcgag ccacaaccca gcgaaataca acgggctgaa gttcaaagcc
     1021 tggtacggcg ggtccgcgct tccagagatg tatgccgcaa tcgccggcgc gctgggccag
     1081 tcttcgaaac gcgacggcgg ttcgatcgaa gagtcggatc tgctcgatga ctacatcgcc
     1141 gcgctgcgcg cacagctcga cgtccgcaag atggcctcgc tgcgcatcct tcacgatccc
     1201 attcacggcg tcgcctccgg cctccccaca cgcatcctcg gcatcgacgt agggacaatt
     1261 cgcggcgaga acaatccttc cttcggcggc gtcaaccctg agccgattcc ggacaacctg
     1321 gcagcgtcca tgcgggagtc gggtgtcggg agtcgggtgt cggagagttc accatccgag
     1381 agcgcatcag ctccgacccc cgacccccga caaccgactc ccgcgaaata cgacctagct
     1441 atctgcaacg acggcgatgc cgaccgcctc ggcattctcg acgagcgggg cgtcttcgtc
     1501 tcgcctcaga agatcatcgc cttgctggcg ctctacctcg tgcgcgaaaa gagaatgacc
     1561 ggcgagatcg tcaagacctt ctcgacatca cgcctcgtcg agacagtggc ccgcaaactc
     1621 ggcgttccct ttcacgagac cgccatcggc ttcaagtacg ttgccgatct catgctcacc
     1681 cgcaacatcc tcatcggcgg cgaggagagc ggcggcatcg gcttcggcca cttccttccc
     1741 gagcgcgacg gcattctctc cggcctgctc gtggccgagt gcatcgccca cttcggcaag
     1801 ccgctgtcgc acttgatcga tgcgatggag acagagttcg gcgcgctgca ctacgaccgg
     1861 cgcgatctca atcgtccgat ggaagtctgc gcacggctga tcgaacgcgt gcgcgccggc
     1921 gagctcaatg ccgtttacgg cgggaccttc gcatctctcg aagagaaaga cggcgtcaaa
     1981 ctcaacttca ccgacggctc atggatcctc ttccgcaagt cgggaaccga gccaatcatc
     2041 cgcatctact gcgagagccc ggatgcgggg caagtcgcgg caatgcttga cgtggcgatc
     2101 gcggagcttg atagaagctg aatcaccaca gagacacaga gagcacagag gttttctctg
     2161 tgtcctctgt gtctctgtgg ttcaaaaaaa acctaaatcg aagccaacac cgtcaacgcc
     2221 gccgccgcgg caacgatgta gctcgctgga tcagtagccg ccgtgacgat cggatcatca
     2281 tcgatctcgc cccgatgcgc gagcagccac atgcgcccga tccagtacat cagcagcgcg
     2341 cagatcagcc agagcgggtc ggtcttcgag tagagctgcg tcacctgcgc gctgttgagg
     2401 tagagcgcaa agaccagcac ggagatcagg ccgctcgacg tgccgaggct taggagaact
     2461 tcgcggtcgc cggtgcggta gctgcggcct cctggcgcct cctcgtcgtt cgagaccgcg
     2521 cgcgtcagat cggcgtagcg ctttgccagc gccaggctga tgaagaggaa actcgaaaac
     2581 gccagcagcc agcgcgag
//
LOCUS       DATLVW010000497         5245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_432080, whole genome shotgun sequence.
ACCESSION   DATLVW010000497 DATLVW010000000
VERSION     DATLVW010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432080"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VNN25_14970"
     CDS             complement(<1..324)
                     /locus_tag="VNN25_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92883.1"
                     /translation="MRTGAAHVRLDAAHVRMDAAHVRTDAAHARMNAAHARTDAARAR
                     MNAAHVRMNAAHARMNAAHARTDAAHVRTDAAHVRTDAAHVRTGAAHVRTDAAHVRTG
                     AAHVRM"
     gene            complement(425..775)
                     /locus_tag="VNN25_14975"
     CDS             complement(425..775)
                     /locus_tag="VNN25_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92884.1"
                     /translation="MRRKRLLWIAPAAIAGMVLFAFIGGEVVRYLWNWLLPGLFGWKQ
                     LTFWQALALLALCRILFGGFGMRGSRDVARSRIRRRVAERWDKMTPEEREKYNQCFRG
                     RWERETMEDSRPSV"
     gene            complement(786..1367)
                     /locus_tag="VNN25_14980"
     CDS             complement(786..1367)
                     /locus_tag="VNN25_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH92885.1"
                     /translation="MADLASVTAMSDDDTRISEIIATERSRLSAFIRRRVPDASDVED
                     ILQEVFYELVEANRLLMPIEHVTGWLFRVARNRIIDLFRKNEPESFTESAVEGEDGEM
                     LQIDDLLPSPDAGPEALYIRHVLLDEVELALNELPDEQRDVFIAHELEGRSFKELSAE
                     TGININTLLSRKRYAVLHLRERLQSIYDEFKNE"
     gene            1458..1982
                     /locus_tag="VNN25_14985"
     CDS             1458..1982
                     /locus_tag="VNN25_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH92886.1"
                     /translation="MEFNLQPTLTGTLLELRPLRPDDFDALFAAASDPLIWEQHPESD
                     RYERTVFQRYFDGAIESRGAFAIIERASGRIIGSSRYWNLKPAESEVEIGWTFLERAF
                     WGGRYNAELKALMLEHAFRFVDRVVFVVGENNLRSQKAVRKLGAELVASEERAAPDGT
                     MRRNVIFALTRSAS"
     gene            complement(1989..3182)
                     /locus_tag="VNN25_14990"
     CDS             complement(1989..3182)
                     /locus_tag="VNN25_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXH92887.1"
                     /translation="MNSEELQLDMNTLHILAFVLSLSAAEALAGECNGWAGARAPVRY
                     ASSADKAVLVRDLDGDGAPEIIASGNHVDELGTFSILANRRDGTFASEKLVTSGFGVM
                     LQDVGDLNHDGVPDLLVSDYFANGIVVYLGDGALQFDGGTRYDTATHGGPSLIADYDH
                     DGNADVISLSFGSGNPVRLHLFHGLGNGALGPKTTFETGLANGEWPSMRTINGALEIL
                     AGERSGHLGLLHFANGALSVSRVVAGPQFDLSSTFGDVNGDGIADIIDTDLDDSGANE
                     PIYVTLANADGSFRERRQLARPRKTTIPAQVRMSDLDGDGHADLVVSDFQTNHLYFYR
                     GNGAGDFEQGVAIDAGGPVNAFDIGDVNGDGYLDLVTANDDHTVSVIINRGPCAPTKH
                     RAAKH"
     gene            3357..3995
                     /locus_tag="VNN25_14995"
     CDS             3357..3995
                     /locus_tag="VNN25_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXH92888.1"
                     /translation="MTEYQLKQAIVRVARRLDQKGILTSTDGNLSARLDDGGTLITPS
                     GCCKGELEADKIVRLFRDGSVRHGRPSSEIALHRMIYDRRPDVRAIVHAHPPYATAYA
                     VAGMALDQPILSEAILSLGRVPVAVYAMPTAEELARSVEPLVAHHDAVLLRFHGAVCF
                     ARDIEQAGYLMETLEHVARIDFIRRALGSNEVIPQNEVQRLEDLRAAMRAAR"
     gene            4019..5176
                     /locus_tag="VNN25_15000"
     CDS             4019..5176
                     /locus_tag="VNN25_15000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXH92889.1"
                     /translation="MTVVNIADLRAAAKKRLPRVVFDYIDGGADAEWTMRENARVFDD
                     VMLRPRSAVATPSVDMRTTVLGATIDLPFLLAPVGSSRLFFPRGEEVAARAAGEAGTI
                     YTLSTLSGCAVGDVKAATRGPVWYQLYVIGGREVAEAGIERAKTACCSALVVTVDTPV
                     AGNRERDVRNGTKELVSGAPFTMFPYLFQFLARPRWLAAFLLDGGLMKFPNIVLKNGP
                     MPYADVGAALEQSMVSWADLAWIRETWQGPIVVKGVHTADDARRAIDHGAAAVIVSNH
                     GGRQLDGVAPTLRVLPEVIAAANGQAEVLIDGGIRRGSDVVKALCIGARAVLVGRAYA
                     YGLGAAGRAGVTRSIDVLRSGIVRTMKLLGCASVRDLDRSFVQMPSAWLEF"
BASE COUNT          873 a         1637 c         1745 g          990 t
ORIGIN      
        1 catccgcacg tgtgcggcgc ccgtccgcac gtgcgcggcg tccgtccgca cgtgcgcggc
       61 gcccgtccgc acgtgcgcgg cgtccgtccg cacgtgcgcg gcgtccgtcc gcacgtgtgc
      121 ggcgtccgtc cgcgcgtgtg cggcgttcat ccgcgcgtgt gcggcgttca tccgcacgtg
      181 cgcggcgttc atccgcgcgc gtgcggcgtc cgtccgcgcg tgtgcggcgt tcatccgcgc
      241 gtgtgcggcg tccgtccgca cgtgtgcggc gtccatccgc acgtgcgcgg cgtccagccg
      301 gacatgcgcg gcgcccgtgc gcacgtgtgc ggcgccttgc cggacctgtg cggcgctcag
      361 ccggcgcggg aaatcaccgc gttcacgggg cgggatctgc ggacggcttc ttcggcgtca
      421 acgctcacac ggaaggccgc gagtcctcca tcgtttcccg ctcccagcgg ccgcgaaagc
      481 actggttgta cttttcccgc tcttcgggcg tcatcttgtc ccagcgctcg gccacgcggc
      541 ggcggatgcg ggagcgcgcg acgtcgcggc tgccgcgcat accgaaacct ccgaacagga
      601 tgcggcagag agccaatagg gcgagggctt gccagaaggt gagctgcttc catccgaaca
      661 gaccgggcag cagccagttc cagagatacc gcaccacttc gccgccgatg aaggcgaaca
      721 gcaccattcc ggcgatggct gccggagcga tccacaaaag tcttttcctt ctcatgtcgt
      781 gctcctcact cgttcttgaa ttcgtcgtaa atgctctgca gccgctcgcg caagtgaagt
      841 accgcgtaac gcttacgcga gagcagggtg ttgatgttga tgccggtttc tgccgacaac
      901 tctttgaagc tgcgcccttc cagttcgtgg gcgatgaaga cgtcgcgctg ttcgtcggga
      961 agctcgttca gcgcgagctc cacctcgtcc agcagcacgt ggcggatgta gagcgcttcc
     1021 ggtccggcgt cgggcgatgg caggagatcg tcgatctgca gcatctcgcc gtcctcgcct
     1081 tcgacggccg attcggtgaa gctctccggc tcgtttttcc ggaagagatc aatgatgcga
     1141 ttgcgggcca cgcgaaagag ccagccggtg acatgctcga tcggcatcag gaggcggttc
     1201 gcttcgacaa gctcgtaaaa gacttcctgg aggatgtctt cgacgtccga ggcgtcgggc
     1261 acgcgccgtc ggataaaggc gctcagccgc gatcgctcag tcgcgatgat ctcggagatt
     1321 cgagtgtcgt catcgctcat cgcagttaca cttgccagat cagccatcca ctggggtaga
     1381 cggatgacgc cgtcggatat tgtgcgccgg gaacactcag tgacctctgt gtctccgtgg
     1441 tgatagggtt ttgcgtcttg gaattcaatc ttcagcccac acttaccgga acgctgctcg
     1501 aactgcgccc tttgcggccg gacgactttg acgcgctctt tgccgcagcg agcgatccgc
     1561 tcatctggga gcagcatccg gagagcgacc ggtacgagcg gacggttttc cagcgctact
     1621 tcgatggtgc catcgagtcg cgcggcgcgt ttgcgatcat cgagcgcgcc tcgggcagga
     1681 ttatcggcag ctcgcgctac tggaacctga aacccgcgga gagcgaggtc gagatcggct
     1741 ggacgtttct ggagcgcgca ttctggggcg gacgctacaa cgcggaactg aaggcgctga
     1801 tgctcgaaca cgcgtttcga ttcgtcgatc gcgtcgtctt cgtagtcggc gagaacaatc
     1861 ttcgatcgca gaaggccgtg cgaaagctgg gcgccgagct cgtagcgtca gaagagcgcg
     1921 ccgcgccgga cggcacgatg cggcgcaacg tcatcttcgc gctcacacgc tcggcctcgt
     1981 agtgcgaatc agtgcttcgc ggcgcggtgt ttcgttggag cgcaagggcc gcggttgatg
     2041 atgactgaca cggtgtgatc gtcgttcgcg gtcacgaggt cgagatagcc gtcgccgttg
     2101 acgtcgccga tgtcgaacgc gttcacgggc ccgccggcgt cgatggccac tccttgctcg
     2161 aaatcgcctg caccattgcc gcgatagaag tagaggtggt tcgtctggaa gtcgctcacg
     2221 acgagatcgg catggccgtc gccgtcgaga tcgctcatgc ggacttgagc ggggattgtg
     2281 gtctttcgcg gacgggcgag ctgtcgccgc tcgcgaaagc tgccatcagc attcgccagc
     2341 gtgacgtaaa tcggttcgtt ggcgccgctg tcatcgaggt cggtgtcgat gatgtcggca
     2401 attccgtcac cgtttacgtc gccgaaggtg ctgctcagat cgaattgagg acctgccaca
     2461 acccgcgaca cggagagtgc accgttcgcg aagtgcagaa ggccgaggtg tccggaacgc
     2521 tcgccggcga ggatctcgag cgcaccgttg atcgtgcgca ttgaaggcca ctcgccgttc
     2581 gcgagaccgg tctcgaaggt cgtcttcggg ccaagcgctc cgttgccgag gccgtgaaag
     2641 agatgcagac gaaccggatt tccggatccg aacgagaggc tgatcacgtc ggcgtttcca
     2701 tcgtggtcgt agtcggcgat cagggaagga ccgccgtgcg tggcggtgtc atagcgagtg
     2761 ccgccgtcga attggagtgc gccgtcgccg aggtagacga cgatgccatt cgcgaagtag
     2821 tcggagacga gaaggtccgg aacgccgtcg tggttcaagt cgcccacgtc ctgcagcatc
     2881 actccgaagc cgctcgtcac cagtttttcc gaagcgaagg tgccatcgcg acgattcgca
     2941 agaatcgaaa acgttcccag ctcgtcgacg tgattgccgg acgcaatgat ctcgggcgct
     3001 ccgtcgccgt cgagatcgcg cacgagcacg gctttgtccg ccgaggaggc gtaacgcacc
     3061 ggtgcccggg cgcccgccca gccgttgcac tcccccgcaa gcgcctcggc ggcactgagc
     3121 gagagaacga aagcgagaat gtggagagtg ttcatgtcga gctgcaattc ttcgctgttc
     3181 atgggaacga ttcgatgacc attcggtgag attcctcaca aaaaccggtt cacgcggaga
     3241 cgcggggagc gcggagagaa acctctgcgt cctccgcgcc tccgcgtgaa acggtttggg
     3301 cgagacacga cgtttcttca gaacttcagc cagagacggt aagatcgatc cgcttggtga
     3361 ctgaatatca attgaagcag gccatcgtgc gcgtggcgcg gcgtctcgat cagaagggga
     3421 ttctcacttc gacagacggc aatctctcgg cgcggctcga cgacggcgga acgctgatca
     3481 cgccgagcgg ctgctgcaag ggcgagctgg aggcggacaa gatcgttcgg ctgtttcgcg
     3541 atggctcggt gcggcatgga cggccttcgt cggagatcgc cttgcaccgc atgatttacg
     3601 atcgacggcc cgacgttcgc gccatcgtcc acgcgcatcc gccgtatgcg acggcgtacg
     3661 cggtggccgg catggcgctc gatcagccga ttctctccga ggcgattctg tcgctggggc
     3721 gggtaccggt ggcggtgtac gcgatgccga ccgccgagga gctggcgcgc agcgtcgagc
     3781 cgttggtggc ccaccatgat gccgtgctgc tccgctttca cggcgcggtc tgtttcgcgc
     3841 gcgacatcga gcaggccggc tatctgatgg agacgctcga gcacgtcgca cgcatcgact
     3901 tcattcgccg cgccctcggg tcgaacgaag tgatcccgca gaatgaagtg cagcggctgg
     3961 aagacctgcg tgccgcgatg cgcgcggcgc gctgaccgtt cctggtgaga aggaccgcat
     4021 gaccgtcgtc aatatcgcgg atctcagggc cgctgcgaag aagcgcctcc cgcgcgtcgt
     4081 gttcgactac atcgacggcg gtgcggacgc cgagtggacg atgcgcgaga acgcgcgcgt
     4141 attcgacgat gtgatgctgc gccctcgctc tgccgtggcc acgccgagcg tcgacatgcg
     4201 cacgaccgtc ctcggcgcca ccatcgacct tccgtttctt ctcgcgcccg tcggcagttc
     4261 caggctcttc tttccgcgcg gcgaggaagt ggctgcgcgc gccgcgggcg aggccggcac
     4321 gatctatacg ctctcgacgc tgtcgggctg cgcggtcggc gacgtcaagg cggctacgcg
     4381 cgggccggtc tggtatcagc tctatgtaat cggcggccgc gaggtagccg aggccgggat
     4441 cgagcgcgcg aaaaccgcct gctgctcggc gctggtcgtc accgtcgaca cgcccgtcgc
     4501 cggaaatcgg gagcgcgatg tgcgcaatgg aacgaaagag ctggtgtcgg gcgcgccgtt
     4561 tacgatgttc ccttacctct ttcaattcct cgcacgcccg cgctggcttg ccgccttcct
     4621 cctcgacggc ggtctgatga agtttccgaa catcgtgctg aagaatggcc cgatgccgta
     4681 cgcggatgtc ggcgcggccc tcgaacagtc gatggtgtcg tgggctgacc tcgcgtggat
     4741 ccgcgagacc tggcagggtc cgatcgtggt gaagggcgta cacacggcgg acgatgcccg
     4801 ccgcgccatc gatcacggcg ctgccgcggt catcgtctcg aaccatggcg ggcggcaact
     4861 cgacggtgtc gcgccaacgc tccgcgtgct gccggaagtc atcgcggcag cgaacgggca
     4921 ggccgaggtg ctgatcgacg ggggcattcg aagggggagc gatgtggtga aggcgctctg
     4981 cattggcgcg cgtgcggtcc ttgtcggacg tgcttacgcc tacggcctcg gcgcggccgg
     5041 cagagcgggt gtcacgcgat cgatcgacgt tctccgctcc ggcatcgtcc gcacgatgaa
     5101 actcctcggc tgcgcgtcag tccgcgatct cgatcgatcg ttcgtgcaga tgccttccgc
     5161 gtggctcgaa ttctgagagg cgattaccac agagacacag atagcacaga gaaacactga
     5221 ctttctctgt gacctctgcg tctct
//
LOCUS       DATLVW010000498         3782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_230798, whole genome shotgun sequence.
ACCESSION   DATLVW010000498 DATLVW010000000
VERSION     DATLVW010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230798"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VNN25_15005"
     CDS             <1..386
                     /locus_tag="VNN25_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HXH92890.1"
                     /translation="AMFAHETEEIERLAMDAVMKHERALGFETRDVSKENNGYDIESR
                     DPKTGRLRFIEVKGRINGADTVTVTRNEMLCALNSPDQWRLAIVLVDGVAKNPMYIAH
                     PFTREPQFAETSATFSISALTSGLL"
     gene            422..1417
                     /locus_tag="VNN25_15010"
     CDS             422..1417
                     /locus_tag="VNN25_15010"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HXH92891.1"
                     /translation="MDSAGQTLASEEASALDRANALEVLGNWRTAHSFALNSMQLTLR
                     KRAAAVCQKPLIAQRLKRVSSIVAKLRRFPKMNLSRMQDIAGCRAIVDTVDEVRCLEE
                     TYKTSRQKHKLVNEKDYITSPQPSGYRGIHLIYKFESAKSADHTGRLIEVQIRSRLQH
                     AWATAVETVGTFVKQSLKASQGSDDWLRFFSLAASLFAIREGCPTVPDTPEYSDELQL
                     KTRNLFDGLKADTVLSAYSRVIQVFEQKKMNFAYYFLLELRPAEGRLMIESFRRTELG
                     IATKRYLDRERAQDGNSQTVLVVADSIKALKQAYPNYFLDTHEFIKALKSAIGRG"
     gene            1422..>3782
                     /locus_tag="VNN25_15015"
     CDS             1422..>3782
                     /locus_tag="VNN25_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HXH92892.1"
                     /translation="MAAVKRKLIEVALPLEAINRESAREKSIRHGHPSTLHLWWARRP
                     LAACRAVLFASLVNDPSSLPEEFPTEELQEKERQRLFRIIEDLVKWENSNNEEVLDRA
                     RKEILKSTDGNPPPVLDPFCGGGSIPLEAQRLGLEAHGSDLNPVAVLITKALIEIPPK
                     FADLPPVNPEAKKKLTHSAKWKGAQGLADDVRYYGKWMRDQAEKRIGHLYPKVKLEDG
                     REATVIAWLWARTVKCPNPACGIDAPLIRSFALSAKPGREASVRAVAQGHELRYEVVA
                     TVPSREGTVRRSGAECLACGTSIPLEYIRSQGKAKRIRVRMIGIVAEGESGREYLPPS
                     ADQETTAESIVAPSFPTTFLPDEALGFRVQGYGIEQHRELFSARQLLAINTLSDLLVE
                     TRQRIVDDVISLGEATSDREKESQNYADAVLTYLAFAVDKVAEGSTTLCTWSPLPTKL
                     HVVSTFGRQVLPMIWDFAEANIFANSSGNFERMTSLVSEVIAASPLRVTPGQVEQRDA
                     ASLTPSRLSLISTDPPYYDNIAYAELSDFFYVWLRRGLSSIYPSLFSTLLTPKTQELI
                     ASPFRFDGSRVAAKNFFEDGLEQAFRSAAAIASVEYPVSIYYSFKQSEDDDQGAVSST
                     GWETMLSALIRAGFAINGTWPVRAERAGGFRNKDRNSLASTIVLICRQRNPSARIATR
                     REFINALKDELPAALVALQHGNIAPVDLAQVAIGPGMAVFSRYAKVIEADGEPMRVRS
                     ALQLINQALDEVLSEQESEYDADTQWAVAWFDQFGTSEAAFGDAETL"
BASE COUNT          856 a         1045 c         1107 g          774 t
ORIGIN      
        1 ctgcgatgtt cgctcacgaa accgaggaga tcgagcgcct ggcgatggac gcggtgatga
       61 agcacgagcg cgccctcggg ttcgagaccc gcgatgtatc gaaagagaac aacggctacg
      121 acattgaatc gcgcgatcca aaaaccggcc gcttgcgctt tatcgaagta aagggccgca
      181 tcaacggtgc cgacacagtt acggtgactc ggaacgaaat gctctgcgcg ctcaattcgc
      241 cggaccagtg gcggctagcg atcgtgctcg tcgacggcgt tgcaaagaac cctatgtata
      301 tcgcgcatcc gttcacacgc gagccgcagt tcgccgagac aagcgcaacc ttttccatta
      361 gcgctctgac ctcaggactt ttgtgatgat ttggacgacg ccaaagtaca gtaggtccca
      421 agtggactcc gccggccaaa cgctggcttc agaggaagca agcgctcttg acagggccaa
      481 tgcgctggaa gtgctcggaa actggcggac agctcacagt ttcgctctca actcaatgca
      541 actgacacta cggaagagag ccgcggcggt atgtcagaag ccgctaatcg cacagcggct
      601 gaagcgtgtc tcatccatcg tcgccaagct tcgacggttc ccgaagatga atctgtcgcg
      661 aatgcaagat atcgccggat gtcgtgcgat cgttgacacg gtcgacgaag tgcgatgtct
      721 ggaggagacc tataagacga gtcgccaaaa gcacaaactc gtaaacgaaa aggactacat
      781 cacatcgcca cagccatctg ggtatcgagg catccatctc atctataagt tcgagagcgc
      841 aaaatcggcc gatcatacgg gtcgactaat cgaagtccag atcagaagcc gacttcagca
      901 cgcctgggct acagctgtcg agacggtcgg gactttcgtg aagcagtcgt tgaaggcaag
      961 ccagggatct gacgactggt tacgattctt tagtctcgct gcttcgctct ttgcaattcg
     1021 agagggttgc cccactgtac cggacacgcc tgagtactcc gacgaacttc aactaaagac
     1081 acgaaacctt tttgacggcc tcaaagccga tacggtcttg tccgcataca gtcgtgtaat
     1141 tcaagtcttt gaacagaaga agatgaactt cgcgtactac ttccttctcg aattgcggcc
     1201 ggctgaggga cgcttgatga ttgaatcgtt tcggcgcaca gaactcggca tcgcgactaa
     1261 gcgatacctg gatcgagagc gtgcccaaga cggtaactcg cagactgttc tggttgtagc
     1321 cgattcgatc aaggcactga aacaagccta ccccaactac ttcttggata cgcatgagtt
     1381 catcaaggct ttgaaaagtg cgatcgggag ggggtgacag aatggccgcc gtcaagagga
     1441 agttgatcga agtcgctctg cccctcgaag cgatcaaccg cgagtcggcg cgggagaagt
     1501 cgattcggca tggacatccg tcgacgctgc atctttggtg ggcgcggcgg ccgttggcgg
     1561 cttgccgggc ggtcttgttt gcgtcgctgg tgaatgatcc ttccagtctc cctgaggagt
     1621 ttccgaccga ggagctacag gagaaagagc ggcagcggct gttccggata attgaggatc
     1681 tggtgaagtg ggagaactcg aacaacgaag aagttctcga tcgggcgcgg aaggagatcc
     1741 tgaaatcaac agatgggaat ccgccgccgg tgctcgatcc cttctgcgga ggcggatcga
     1801 tcccgctcga ggcgcaacgc cttgggttgg aggcgcacgg gagcgacctg aatcccgtgg
     1861 cggtgctgat cacgaaggcg ctgatcgaga tcccgccgaa gttcgcagac cttcctcccg
     1921 tgaacccgga ggcgaagaag aagttgacgc actccgcgaa atggaaagga gcacaggggc
     1981 ttgccgacga cgttcgctac tacggcaagt ggatgcggga ccaagccgag aagcggatcg
     2041 gacatctcta tccgaaggtg aagttggagg atggtcgcga ggctaccgtc atcgcgtggc
     2101 tttgggcgag aacggtgaag tgcccgaacc cagcatgtgg gatagatgcg ccgctcatcc
     2161 gttccttcgc gctttcagcg aagcccggcc gcgaggcctc tgttcgcgca gtcgctcaag
     2221 gccacgagct ccgatacgaa gtcgttgcaa cggtaccgtc ccgagaaggc acggttcgac
     2281 ggagcggcgc agaatgtctc gcctgcggta cgagtattcc tctcgaatac atacgttctc
     2341 aagggaaagc aaaacggatt cgcgtacgga tgattggcat tgtcgccgaa ggagagagcg
     2401 gtcgcgagta cttgccaccg tcggcggatc aggagaccac ggccgaatca atagttgctc
     2461 cctcttttcc gactacattc ctgcccgacg aagcgcttgg atttcgagtc caagggtacg
     2521 gcatcgagca gcatcgagag ctcttcagtg ctcgccaact gctcgccata aatactctct
     2581 ccgatctgct tgtcgagaca cgccagcgaa ttgtcgatga tgtcatctcg cttggcgagg
     2641 ccacgtcgga tcgcgaaaag gagtctcaga actatgcgga tgcggtcctg acttacctcg
     2701 cattcgcggt cgacaaggtt gccgagggca gtaccacgct gtgcacgtgg agtccgctac
     2761 ctacgaagct ccatgtcgtc agcacgttcg gccgccaggt tttgccaatg atctgggact
     2821 ttgccgaagc gaacatcttt gccaactcct cgggtaattt tgaacgaatg acgtcgttgg
     2881 tctccgaagt catcgcagca tcaccgctca gagttacccc gggacaagtc gagcagcgtg
     2941 atgccgcgtc gcttactccg agtcgtctaa gcctgatatc aacagatccg ccttattacg
     3001 acaatatcgc ttatgccgag ctttccgact tcttctatgt ttggcttcga agaggcctta
     3061 gctctattta cccgagcctt ttttccacgc tactcactcc caagacgcag gagctgatcg
     3121 cctccccttt tcgattcgac ggcagtcgtg tcgcggcgaa gaacttcttt gaggatgggt
     3181 tagagcaagc ctttcggagt gctgccgcga tcgcaagcgt agagtatccc gtgagcatct
     3241 actactcatt caagcagtcc gaagatgacg atcaaggtgc ggtctcatca accggttggg
     3301 aaacgatgct ttcagcgctc atcagagcgg ggtttgcgat caatggcacg tggccggtgc
     3361 gtgcggagcg agccggcggc ttccggaaca aagaccggaa ctcgctagcg tcaaccattg
     3421 tgcttatttg tcgccagcga aatccatccg ctcggatcgc aacgagacga gagtttatca
     3481 acgctctaaa ggatgaattg cccgccgcgt tggtggcgct gcagcacggc aacatcgccc
     3541 ctgtcgacct cgcgcaggtc gcaattggac cgggtatggc tgtcttctcg cgatacgcaa
     3601 aagtcatcga agccgatggt gagccgatgc gcgttcgcag cgcgttgcag ctcatcaacc
     3661 aagctctgga tgaggtgctc tccgaacagg agagcgagta cgacgccgac acgcagtggg
     3721 cggtcgcgtg gttcgatcaa ttcgggacga gcgaggcagc attcggcgat gcggagacgc
     3781 tc
//
LOCUS       DATLVW010000499         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_432125, whole genome shotgun sequence.
ACCESSION   DATLVW010000499 DATLVW010000000
VERSION     DATLVW010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432125"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VNN25_15020"
     CDS             complement(<1..210)
                     /locus_tag="VNN25_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXH92893.1"
                     /translation="MYHQTYNPTGNVVLSTVVAAIPIVVLLYFIALHPHRDRKGARHL
                     GISAPYAAFYAVIAAMLVACLVFRMP"
     gene            325..912
                     /locus_tag="VNN25_15025"
     CDS             325..912
                     /locus_tag="VNN25_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH92894.1"
                     /translation="MTTNPHPRLTPEEYLRIERAAEWKSEYIDGEMFAMAGASMRHVL
                     ITTNLVGELRDRLREGPCTVYGAELRVATDRLRHYTYPDVVVVCDPVEVVDEHRDTLT
                     NPKVIAEVLSDSTEKYDRGAKFERYRAVPTLSEYLLVSQDRVHIELYTRQPDGGWFLR
                     EWNDPDAEIDLTSLHCRLKIAEVYAKVTFGDQATS"
     gene            1077..>2974
                     /locus_tag="VNN25_15030"
     CDS             1077..>2974
                     /locus_tag="VNN25_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92895.1"
                     /translation="MITGRSVATFVAFLLAAEFGLAQTPAAPPLAISGDNPFAIDPAT
                     NSGLSVFWLKNTSKKTVPLVLGVGQYTSDVTHQIIPGKGVVGAIDGLPAPFIYKAPSN
                     LAPGAQVPVKITVTDFLSVGGATAVLTNQGAPIGTVRVIKDRFPFKVAPPGWKEGVPY
                     PLRISADNAGRITLLNEEPVRYVVAWTFSAPGIDNDSDEITIPPNSGRTIVLHPKQNE
                     SLSGDTLGSLFRDRESDGWLTLSAIPAEKAQFLSWWPSRHLPIKLSRSWWSDVWRPWA
                     SNALIVILLLLGGICSLILTNWVPNRTLKLNLQRRLEEIAEQIRTISVACDSMLRVSI
                     RVERLRIKDSLNSRKAFSADYPALAQACETDIARLERLVGVVQRIDAIYRSANSTLLG
                     FAPSIMRNAIHSGLRAEMQVTAPKVTDGALDDATKTVTDAAAVLAGLQTPNDEFKKKV
                     TARVEKIVSDPRFTTSKFLIYARGKIPNLDSCVNATNPHAENMTDDNVCAIDSAVTKY
                     EILIDVADIIDNLGDQRSKWNDVIERLLKNLRADNMALLRRADIRALELREDVHPDDV
                     ITKLKDGRFSIVCDPSLPAAGDTARFRVVLFDRLISNSSARDEIMCQWDFGDGLQESG
                     WQVGHYYR"
BASE COUNT          619 a          869 c          885 g          601 t
ORIGIN      
        1 cggcatcctg aagacgagac acgccacgag catcgccgca atgaccgcgt agaaagcggc
       61 gtacggcgca gaaatgccga ggtgccgcgc ccctttccga tcgcggtgag gatggagcgc
      121 gatgaagtag agcaatacga cgatgggaat cgcggccacg accgtcgaga ggaccacgtt
      181 gccggtcggg ttgtaggtct ggtggtacat gtgcggtgaa ggagtgtaaa tccgcggatg
      241 agttttgccg aggaggatcg ttggtcgtta tcgccgcagg cttgagtgct cccgtctctg
      301 aaaatgaatt attcttgctc cggcatgacc acgaatcccc accctcgcct aactcccgag
      361 gaatacctgc gaatcgagcg cgccgcggag tggaagagtg aatacatcga cggcgagatg
      421 ttcgcgatgg caggtgcgtc aatgcgtcac gtgctcatca cgacaaacct cgtcggcgaa
      481 cttcgggacc gtttgcgcga agggccgtgc acggtttacg gcgctgagct gcgcgtcgcg
      541 actgaccgtc ttcgccacta tacgtacccc gatgtcgtcg tggtctgcga tcctgtcgaa
      601 gtcgtcgatg agcaccgcga tactctcacc aatcccaagg tgatcgcgga ggtgctttcc
      661 gactcaacgg agaaatacga tcgcggagcg aaattcgagc gctaccgcgc cgtgcccacg
      721 ctttccgaat acctgctggt ttctcaggat cgcgtccaca tcgagctcta cacacgccag
      781 cccgacggtg gatggttcct gcgcgagtgg aacgacccgg acgccgagat cgatctcacc
      841 tcacttcact gccgcctgaa gatcgccgag gtgtacgcga aggtgacttt cggcgatcag
      901 gcgacaagtt gaaggggctt ctcaggtcga cccgtaagtg ggctaacttc gaggctgttc
      961 aggttgaacc gaccgaggaa gaggtgctcc atccccatgc cgcaagcgac tttctcaacg
     1021 ggagggtagg aaagtgcagc gccgcgtcac tcctaagtga gggggggcct ccgacagtga
     1081 tcacaggacg atctgttgcc acgtttgttg ctttcttact agccgcagaa ttcgggctgg
     1141 cgcaaacgcc agcggcgccg ccgctggcga tctctggcga taaccctttc gccatcgatc
     1201 ccgcgactaa cagtggcctc tccgtgttct ggctcaaaaa tacgtcgaag aagaccgtcc
     1261 cactggtgct gggagtcggt cagtacacat cggacgttac gcaccagatc attcccggaa
     1321 agggagtcgt aggagcgatc gatgggttgc cagcgccatt catctacaag gccccgtcca
     1381 acctggcgcc tggagcgcag gttcccgtca aaatcacagt caccgatttt ctatcggtcg
     1441 gtggagcaac ggcggttttg accaatcaag gggccccgat cggcaccgtt cgtgtgatca
     1501 aggacaggtt tccgttcaaa gtcgcgcccc ccggatggaa ggaaggtgtc ccctatccgt
     1561 tgcgtatttc cgcagacaac gcggggcgca tcacgttgct taatgaggag ccggtgcggt
     1621 atgtcgtcgc ctggaccttt tcggcgcccg gaatagacaa tgacagcgat gaaatcacca
     1681 tcccgccgaa ttccgggcga accatcgttc ttcatccgaa acaaaacgaa tccctgtcag
     1741 gcgacacgct cggctcgttg tttcgcgacc gcgagtccga tggatggctg acgctcagcg
     1801 ccatcccggc ggaaaaggcc cagttcctat cgtggtggcc gtcccgtcat ctgccgatta
     1861 agctttcgcg ttcttggtgg tctgacgtct ggcggccgtg ggcatcgaac gcgctcatcg
     1921 tgatcctcct actcctcggc gggatctgct cgctgattct gacgaattgg gtgccgaacc
     1981 gtactctcaa gttgaatttg cagcgccggc tggaagaaat cgccgaacag attcgcacca
     2041 tttcagttgc gtgcgactcc atgttacgcg tttcgatacg ggtggaacgg cttcggatta
     2101 aggactcgct caattcccgc aaggcgttca gtgccgatta tccagcgctg gcgcaagcct
     2161 gcgagacgga catcgcccgt ctcgagcgtc tggttggcgt tgtgcagagg attgacgcga
     2221 tctaccgctc ggcgaacagt acgctcctcg gctttgcccc gtcgatcatg cgcaacgcca
     2281 tccacagtgg gctccgggcg gagatgcagg ttacagcgcc gaaggtgact gacggtgccc
     2341 tcgatgatgc gacgaagacc gtgacggacg ccgccgcggt cctggcgggg ctccagactc
     2401 ccaatgacga gttcaagaag aaagtcaccg cgcgcgtcga gaaaattgtc agtgatccac
     2461 ggtttaccac cagcaagttc ctcatttacg ccaggggaaa aataccgaat ctggattcgt
     2521 gtgtgaatgc tacaaacccg catgcagaga acatgacgga tgacaacgtc tgcgccatcg
     2581 actcggcggt cacaaaatac gagatcctga tcgacgtcgc ggacatcatc gacaacttgg
     2641 gcgaccaacg ttcgaaatgg aacgacgtga tcgagcggct gttgaagaac ctccgcgctg
     2701 ataacatggc gctcctgcgg cgcgctgata ttcgcgccct cgagttgcgc gaggatgtcc
     2761 atccggatga tgtgatcacc aagctcaagg acggaaggtt ctccattgtc tgcgatccaa
     2821 gcctgccggc tgcaggggac acggcccggt ttcgggtcgt ccttttcgat cgcctgatct
     2881 ccaattccag cgcccgcgac gagatcatgt gccaatggga cttcggcgat ggcctgcaag
     2941 agtcaggatg gcaggttggt cactactatc gcca
//
LOCUS       DATLVW010000500         3171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_432352, whole genome shotgun sequence.
ACCESSION   DATLVW010000500 DATLVW010000000
VERSION     DATLVW010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432352"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..>3171
                     /locus_tag="VNN25_15035"
     CDS             545..>3171
                     /locus_tag="VNN25_15035"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HXH92896.1"
                     /translation="MKSIRFAVSIVFLLASAVSATVFQPTSDRQLTDRSDSVVVATVR
                     DATSHVRADGYVVTDYRFEIEETLKGTAAGTIIVSEIGGRSGDRVTFISDSAAYTSGE
                     HVLVFLKKRSDGTYFTTSMAMGKFSFTRNASGEAVVTREVSELRDDPARLANEFKQFV
                     QGKTSASYATTLTPITMSLRPTPLAAPTAATYCLTFGMPLRWPGGEFGTVTFSVDGTL
                     SAVPNAAASIANGMAAWTSDPNAGITLNGGGPRPVGSTASPSFDHNNVIYLNQTPAPN
                     TGFCDAADVCTIVSGNTDPGPANQHTYKGEQFLSIQDADIVFGSGSPSTFDALITHEL
                     GHAIGFRHSNQTGDGTGVCASPSPCSSSALMTSPVNAGFGSNLQQWDHDAVETVYGNG
                     PPCQPPTSVSISGGGAYPSGQTATLSANVVGGNGTFTYAWFDGLEPDNSHPVGTNSST
                     FTTPPITGTKNYWVKVSNTCGNVTASASVSVQTGPGCTAASIAVQPTSTTISLGGTAT
                     LSVTAGGSSPFSYQWYQATSNLDTSHPVGLNSPSFTTPGLTQATSYWVSVTNGCGFAK
                     SNVATVSIAGQCTLPSFSIQPASFIPTPGTPTYLFAFAAGVSSYQWYKGNTGDTSTPV
                     NGSGPSNDRWVNQIFIDLLGRTADAASLSGFSTLLGGGTPRTTVALTVLTSTEYRNRL
                     MTSFYATFLHRTPSAAELSFWLPAFAANLSDEQIEAQLVSSPEYFALSGGTNAGWITR
                     IFNDVLGRTPSAAETAAYSGFLGSSSRVTVGLSILKSSEAVNRRVQQYYAQFLHRNAT
                     ASEAAALVPAILGGVSDEQVIAQIVGADEYFNFGTLLVVDPITVTTRYWVRATNACGP
                     TDSNTATLTVPQCATPIIL"
BASE COUNT          641 a         1092 c          857 g          581 t
ORIGIN      
        1 cttcaggaca ttcgtcatca aacttcatga ctctcgtcat caaacttcat gactctcatc
       61 atcaaacttc atgactctgg tcatcaaact tcatgactct cgtcatcaaa cttcatcggc
      121 gagatctcca cagaacgcca ccgccaagtc cttctcttca ttgaagtagc ggcaatcaac
      181 ccaacagaca acgcgcgaag cgcaaccaac cgacaaccga caacgcggcg atccgtacta
      241 gtgccggacc gcccgcgaac gccctttcgc cgcagcttcc cggcaggtcg ccaccctgtt
      301 gcggcgcctg agcctggatc gacgcaacca acagaaggat cgacaggcag cacaccgcgc
      361 ctcggagcat gaacacgatt ctcgtcagtt ggcggaggag ttttgtaact gtggtcacag
      421 cccctcaccg ctttcggaac gagattgcta cgactacaat gctcaggtta ttcaaataag
      481 taacggaaac aaatccactg gccgctccgg ccaccgcaat caaccaacag agagaggtcc
      541 gttcttgaaa tcgatccggt tcgctgtatc catcgtcttt cttcttgcct cggccgtctc
      601 ggcaacggtg tttcagccca ccagcgaccg ccaactgacc gaccgctccg attcggttgt
      661 cgtcgctacc gtgcgcgatg ccacatccca cgttcgcgcc gacggatatg tcgtcaccga
      721 ctatcgcttc gagatcgagg agacgctcaa aggcacggcg gcaggcacga tcatcgtcag
      781 cgagatcggc ggacggagcg gcgatcgcgt caccttcatc agcgactccg ccgcttacac
      841 gtccggcgag cacgtcctgg tcttcctgaa aaagcgcagc gacgggacgt acttcacgac
      901 gtcgatggcg atgggcaagt tctcgttcac gcgcaacgcc agcggggaag cggtggtgac
      961 gcgcgaggtg tccgaacttc gcgatgaccc ggcgcggctg gcgaatgagt tcaagcagtt
     1021 cgtgcagggg aagacgagcg cgtcgtacgc gacgacgttg accccgatca cgatgtctct
     1081 tcgtcccacg ccgctcgctg ctccgaccgc ggccacgtac tgcctcacgt ttggaatgcc
     1141 gctgcgctgg cccggggggg agtttggaac cgtcaccttc tccgttgatg gaacgctttc
     1201 agctgttcca aatgctgcgg caagcattgc gaacggtatg gccgcgtgga ccagtgatcc
     1261 gaacgcggga atcaccttga acggtggtgg ccctagaccg gtaggttcta cggcgtcgcc
     1321 gtcgttcgat cacaacaacg tgatctatct gaaccagacg cctgctccga acacgggctt
     1381 ctgtgacgca gccgacgtat gcacaatcgt cagcggcaat acggacccgg gtccagccaa
     1441 ccaacatacg tataagggcg agcagtttct ctccattcag gatgctgaca ttgtgttcgg
     1501 ctctggtagt ccctcaacgt tcgacgcgct gatcactcac gagctcggcc acgccatcgg
     1561 cttccggcac tcaaatcaaa ctggagacgg aaccggcgtc tgcgcttctc catcgccctg
     1621 cagcagcagc gcgctcatga cgtctccggt caacgccggg ttcggctcca accttcagca
     1681 gtgggaccac gatgcagtcg agacggtcta cggcaacggc cccccatgtc agcctcccac
     1741 ctcggtctcc atctccggcg gcggcgccta tccgtccggt caaacggcaa cgctgagcgc
     1801 caacgtcgtc ggcggcaatg gcaccttcac ctacgcatgg ttcgacggct tggagcctga
     1861 caactcgcat cccgtcggca ccaacagctc caccttcacc acgcctccga tcaccgggac
     1921 caagaactac tgggtcaagg tctcgaatac atgcggcaac gtcacggcca gcgcgtccgt
     1981 cagtgtgcag accgggcctg gatgcacggc tgcaagcatc gccgtccagc cgacttcgac
     2041 gaccatcagc ctcggaggca cggccacact ctccgtgacc gcgggtggct cgtcgccgtt
     2101 cagctatcag tggtaccagg caaccagcaa cctcgacacc agccacccgg tcggattgaa
     2161 cagcccgagc ttcacaacgc cgggtctgac gcaggcgacg tcgtactggg tcagcgtcac
     2221 gaatggatgc ggtttcgcga agagcaacgt cgccacggtc agcatcgcgg gacaatgcac
     2281 tctgccgtcc ttctcgattc aacccgccag cttcattccg acgcccggga ctccgaccta
     2341 cctgttcgcc ttcgccgccg gcgtcagttc ttaccagtgg tacaagggaa atacgggcga
     2401 tacctcgaca cccgtcaacg gatcaggccc gtcgaacgac cgttgggtca accagatctt
     2461 catcgatctg ctcggacgaa ccgccgacgc tgcgtcccta tccggcttca gcacactcct
     2521 cggcggcggc acaccgcgaa cgaccgtcgc gctcaccgtc ctgacgtcga ccgaataccg
     2581 caaccggctg atgacgagct tctacgcaac gttcctgcac cgcacgccgt ccgccgcgga
     2641 gctgagcttc tggctccccg ccttcgccgc caatctgagt gacgagcaga tcgaagcgca
     2701 gctcgtttcg tcaccggagt acttcgcgct ctccggcggc acgaacgccg gctggatcac
     2761 acgcatcttc aacgacgtcc tcggccgcac accttcggct gccgagaccg ccgcatacag
     2821 cggctttctg ggcagttcat cgcgcgtaac cgtcggcctc tccatcctca aatcgagcga
     2881 agcggtcaac cggcgcgtac agcagtacta cgcgcagttc ctgcaccgta acgctacggc
     2941 atccgaagcg gcggcgctcg tgccggcgat tctgggtggc gtgagcgacg agcaggtcat
     3001 cgcgcagatc gtgggcgccg acgaatactt caacttcggc acgctcctcg tggtcgatcc
     3061 gatcaccgtg accacccgct actgggttcg cgcgaccaac gcatgcggcc cgaccgacag
     3121 caacacggcc actctcacgg ttccgcagtg cgcaactccc atcattctcg a
//
LOCUS       DATLVW010000501         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_311646, whole genome shotgun sequence.
ACCESSION   DATLVW010000501 DATLVW010000000
VERSION     DATLVW010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311646"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VNN25_15040"
     CDS             complement(<1..793)
                     /locus_tag="VNN25_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HXH92897.1"
                     /translation="MSVQKTLSLAGFLALSLLWAGLIAAQPHLVKDINLLLSPVSSEP
                     GGFASIGNVTYFFANDGVSGNELWKTDGTTIGTVLVRDIRPGPQSGVPNDRVAASVVG
                     NRMLFVATDGIHGLEVWSTDGTADGTVMLADIAPGTASSNPVLGPVAGSTAYFTATAP
                     ATGTELWQTDGTIAGTRLVQDLNPGANSSSPRMLGAFGPLLVFQAFDGTKTTLFTTDG
                     TVEGTKALGPFSGVSGIALGTSFLFAVTTGTGKWDLMKTDGTSSGT"
     gene            complement(842..1291)
                     /locus_tag="VNN25_15045"
     CDS             complement(842..1291)
                     /locus_tag="VNN25_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH92898.1"
                     /translation="MVSRAAELIETLNLEPHPEGGFYRQVYRSPHSVQPDDGRDTRSA
                     LTAIYFLLPAGEKSRWHRVQSDEIWTHLEGSPVTLHLFDGMDASSVMVDRMHVVPANI
                     WQAAEPSGDYALVACFVAPGFEFSDFSMMSDEADAIARLPQELRRLV"
     gene            complement(1298..1651)
                     /locus_tag="VNN25_15050"
     CDS             complement(1298..1651)
                     /locus_tag="VNN25_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020428978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HXH92899.1"
                     /translation="MKPADIKPEIPFATLASVDIRVGTIQSVHDVAGSSKLIRMVVDF
                     GDHTRNILAGMKKERSDVQAIVGKQALFVVNLAPRTMAGEVSEGMLFDVGFADGIEPA
                     LLSPERPVPNGTRAG"
     gene            complement(1632..1925)
                     /locus_tag="VNN25_15055"
     CDS             complement(1632..1925)
                     /locus_tag="VNN25_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92900.1"
                     /translation="MNVLTSIFSVRSLAAGMMLAFAAVLLIILIVSFRSRAVVFCQYL
                     QAMTGVKLKPYQVAKVYRERGPDGVREFFLDLIIKEDLKQGPLVIPSDEASGH"
     gene            2121..>2808
                     /locus_tag="VNN25_15060"
     CDS             2121..>2808
                     /locus_tag="VNN25_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92901.1"
                     /translation="MVFSTLSFLPIPGNIFFGTAVPVAIAGASSYQTLRREAEVAIGE
                     RSYARAHELYAKVKQESLLPEEKRWVRFRLADTSWRAEANDPKSDDTDVAKARDELLA
                     LVREAGDDHDKLWAEVNESLGDFASSPVSPGLSAMPYYEAALDWWAGSDDIDHARTHY
                     LGIVRSFTLGDDDYLYGVPQQISIDVFENAAKIAVTPADRQTFHYILGVRLFNSGDQD
                     RVGEELEAAIR"
BASE COUNT          556 a          881 c          859 g          512 t
ORIGIN      
        1 tggttccgct ggaggtgcca tccgttttca tcaagtccca cttgccggtt ccggtggtga
       61 ccgcgaaaag aaatgacgtg ccgagggcga tgcccgacac gccggaaaaa gggccgagcg
      121 ctttcgttcc ttcgacagtg ccgtcagtcg tgaagagagt tgtcttggtt ccgtcgaacg
      181 cctggaagac gagcagcggg ccgaacgctc ccagcatgcg cggcgacgaa ctgttcgcac
      241 cgggattcag atcctgaacc aggcgcgtac cagcgattgt cccgtccgtt tgccacagtt
      301 ccgttcccgt cgcaggcgca gtggcggtga agtacgcggt cgatccggcc accgggccta
      361 agacaggatt tgacgaagcc gttcccggag cgatgtcggc cagcatcacc gttccatccg
      421 cagtaccgtc agtggaccag acttccagtc catgaatgcc gtcagtggcg acgaagagca
      481 tccgattccc gacgacagac gccgcaacgc ggtcgttggg aacgccagac tgcggcccgg
      541 gtcggatgtc gcgcacgagc accgtgccga ttgtcgtgcc gtcggtcttc cacagctcgt
      601 tcccggatac cccatcgtta gcgaagaagt aagtaacgtt gccgatggac gcgaatccac
      661 cgggctccga actgaccggc gacagcagga ggttgatgtc tttgacgaga tgcggttgcg
      721 ctgcgatcag gcccgcccat aggagagaga gggccaaaaa gccggcaagg gagagcgtct
      781 tttgtacgga cacacggaaa tgatacgcgg gttcgcgagc ctggcacagc cgtcaggtga
      841 gtcaaaccag tcgtcgcagt tcctgaggca accgcgcaat cgcatcggcc tcatcgctca
      901 tcatcgagaa gtcgctgaac tcgaagccgg gcgcgacgaa gcaggccacc agcgcgtagt
      961 cgccgctcgg ttcggcggct tgccagatgt tggcgggaac gacgtgcatt cgatcgacca
     1021 tcacgctcga cgcgtccatc ccgtcgaaca gatgcagcgt cactggcgag ccttcgaggt
     1081 gcgtccagat ctcatcggac tgcacgcggt gccagcggct cttctcgccc gcgggcagca
     1141 ggaagtagat cgcagtcaac gcgctgcgcg tatcgcggcc atcgtccggt tgtacagaat
     1201 gcggcgaccg gtagacctga cgatagaacc ctccctcggg atgaggttcg agattcagcg
     1261 tctcgatcag ctccgcggcg cgcgagacca tcgcaaacta tcccgctcgc gttccgttcg
     1321 gaaccggccg ctccggactc agaagcgccg gctcgatgcc atccgcgaat cccacgtcga
     1381 acagcatccc ctcggagact tcacccgcca tcgtgcgcgg cgcaaggttc acaacgaaga
     1441 gcgcctgctt gccgacgatg gcttggacgt cgctccgttc cttcttcatg ccggcgagga
     1501 tgttccgcgt gtgatcgccg aagtcgacga ccatgcggat cagtttcgac gagccggcga
     1561 cgtcatgaac ggactgaatc gtgccgacgc ggatgtcgac cgaggcgaga gtcgcgaacg
     1621 gaatctcggg cttaatgtcc gctggcttca tcagaaggga tcaccagcgg cccctgcttg
     1681 aggtcttctt tgatgatgag atcgaggaag aactcgcgca cgccgtcggg gccgcgctcg
     1741 cgatagacct tcgccacctg atacggcttc agcttgaccc ccgtcattgc ctgcaggtac
     1801 tggcagaaga cgacggcgcg cgagcggaac gagacgatga ggatgatcag cagcaccgcc
     1861 gcgaaggcga gcatcatccc cgcagcgagc gagcgaacgc tgaaaatcga cgttagaaca
     1921 ttcattccgg acccgcgatg atcctaccac gcgtggaatg ggtcaggtct aaagtctaaa
     1981 gtgccgaatt ggcctgatcg ccttagcgac gatcgccctc ggcactttag actttagacc
     2041 tgaccctcgg tttccttttc ttccaatccc tcatctaatc agccgtgtag cacctggggg
     2101 taaccgtgaa tctcgccgcg ttggtctttt ccacactctc gttccttccg atccccggca
     2161 atatcttctt cggcacagcg gtgcccgtcg caatcgccgg cgcatcgagc taccaaacgc
     2221 tgcggcgtga ggccgaggtc gcgattgggg agcggtcata cgcgcgcgcg catgagctgt
     2281 acgcgaaggt gaagcaggag tcgctgctgc ccgaagagaa acgctgggtg cgattccgcc
     2341 tcgcggatac atcctggcgc gcggaagcga acgatccgaa gagcgacgat accgacgtcg
     2401 ccaaggcgcg cgatgagctg ctcgcgctcg ttcgcgaggc cggcgacgat cacgacaaac
     2461 tatgggccga ggtcaacgaa tcgctgggcg actttgcgag ctcaccggtc agccccgggc
     2521 tctccgcgat gccgtactac gaagccgcgc tcgactggtg ggctggcagc gacgacatcg
     2581 atcacgcccg cacgcactac ctcggcatcg tgcgcagttt cacattgggt gatgacgact
     2641 atctctatgg tgtcccgcag cagatctcga tcgatgtctt cgagaatgcg gcgaagatcg
     2701 ccgtcactcc ggccgaccgc cagacatttc actacatcct cggcgttcgt cttttcaact
     2761 cgggcgatca ggaccgcgtc ggcgaggagc tcgaggcggc catccgcg
//
LOCUS       DATLVW010000502         9827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_147967, whole genome shotgun sequence.
ACCESSION   DATLVW010000502 DATLVW010000000
VERSION     DATLVW010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147967"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VNN25_15065"
     CDS             <1..670
                     /locus_tag="VNN25_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXH92902.1"
                     /translation="VAQTLLSVRLTPAERLENSPILNVLIATLMFWFLITQFRDKGGL
                     AALDLNNFNFAFLAAGLLLHWRPRSFLRAVTKSVPATAGVLIQFPFYGGIFGMISKTP
                     IAAHLAHAFVSVTTRSTFPVVVAIYSAILGMFVPSGGGKWVVEAPYVMAAANAWHMHL
                     GWTVQIYNAGEALPNLINPFWMLPLMGILGVRARDLAGYSILQMLFHVPIVILLCWLF
                     AQTL"
     gene            complement(934..1299)
                     /locus_tag="VNN25_15070"
     CDS             complement(934..1299)
                     /locus_tag="VNN25_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92903.1"
                     /translation="MSTGYEHNQKNHITFDIIDRHIQSFDLTAHTAPGGVQASRADRL
                     STTYAVARPILVGLAAIPLIPVAWRAALRVFLTALDEVTATIKPAEVKEAFKAGKDQL
                     AGDPEPGAFVEMEPKLPVG"
     gene            complement(1394..2506)
                     /locus_tag="VNN25_15075"
     CDS             complement(1394..2506)
                     /locus_tag="VNN25_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HXH92904.1"
                     /translation="MRNITQSTCCVIPPHIQNHVAQHGNSKQRERAAATLAHTVAQRS
                     CEQAAALIGPRVQRPLEKKRHIYHAGGQLTLPGTLVMTEERTTASDIEMREAFDGSGA
                     VYDFLGSVFLRHSIDNRGMRLDSTVHYGTHFENAFWNGRQMVYGDGDGQLYNRFTAPV
                     DVVAHEHIHGMTEHDAGLLYCGQPGALNEHISDVIGILTKQWRLGLTAMRSDWVIGRE
                     VFGPAVRGVGLRSMKAPGTAYDDPVLGKDPQPSHMRRYMDFPDDDGGVHINSGILNRA
                     FYFTAADLRGYAWEIGGRIWYRTATSRLHPEADFQYFAEATVTVAGEMYGIGGNVQRV
                     IFDAFSRVGLSLPASLLRSAGFGRKALLPEGSSAKE"
     gene            complement(2594..3166)
                     /locus_tag="VNN25_15080"
     CDS             complement(2594..3166)
                     /locus_tag="VNN25_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92905.1"
                     /translation="MRKRISRQQSAVAEPVPVPDPVITPYEKIAEQLGAAINAAVVQI
                     PGYNDDLSETPKNLRRRVTAKFIGMTVAAVESSPELQGVNQLDTMECRDTLQFSQGIQ
                     SLTDQLFGTARRLDLMRRVKEARAGKSALAIYNIAQRVALNPNNTHMKVHVENLRAEL
                     ASPKRVQPAKPPAEADPGTPDPTAKGGGKD"
     gene            3824..4666
                     /locus_tag="VNN25_15085"
     CDS             3824..4666
                     /locus_tag="VNN25_15085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH92906.1"
                     /translation="MAALGLKPFHVIQTANRIAAQMGLRHAISRQHFLKLRIGERNAT
                     EEKILLLVMAFRELSGYAVRASDLFALEPSAAVGDAPLLTPCISAHGSNALVPISSAG
                     AGFRARRISVSDRTGTSGDDEFEALYTEYGLLLRSIAMRRYGIPPDDAEALTHDTFVA
                     YLQRHTKINDLKPWLMGAVCNACKHYWRDRKREMPLPEDFEETIDPTANCSADSWAWR
                     ITVAGLLARLGNQCRETLKRYYWSDESKEAIAATLSLSPGYVLHLLSSCRQRMREMLK
                     RRTS"
     gene            4663..6000
                     /locus_tag="VNN25_15090"
     CDS             4663..6000
                     /locus_tag="VNN25_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92907.1"
                     /translation="MTHPDEAVLSAFSLDAESVADREVVAAHVADCEACQEAIAAFRK
                     FEDALRSPETWMHLTALQSPGDRFAEAEALRRRVQKEDDEASRILASLVNSPLRFRNA
                     KLEEKPGCHTEGMVRMLCAAANERHESRPTFSLELTVTACTIAKALPQTNGAGRRLSM
                     GMALRERANALRYLGKFNDALLALEYAERFFDTSPGADDFDLAIVWYIRAVVFNKSDR
                     LPEGIIAARDAARVFREYGDRDRELSSIMVEAGCLLLSGNAQDAVEAFGRVADLARER
                     SNVRLLASALQNGAAALADLGQLERAERLYSEAIVLYDELRVPTEHARAVWALGSVAV
                     AAGNLEEGAARLDASRKELAHLGLTNDAAQATLEWAEARLALNKPEGVAAACRKIVIV
                     FNSEGMQHHAKEALATLHEALRAGKATPELVRSVRAYLDQLPANPTQPFVMAS"
     gene            6099..6311
                     /locus_tag="VNN25_15095"
     CDS             6099..6311
                     /locus_tag="VNN25_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92908.1"
                     /translation="MQRIRIFVAVVSLFGMLGLTGGAIAAQKKNDNPPARATQSHAQK
                     EMKAPRKGWIAKILDDLENKLSFPPG"
     gene            6391..6609
                     /locus_tag="VNN25_15100"
     CDS             6391..6609
                     /locus_tag="VNN25_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92909.1"
                     /translation="MEIDRGQLRAIVRRSLARYRGGSFALGDSGGARGMLFFTDVEGR
                     EFLCGGLNVDNAQPLCDALNAMLRLVEK"
     gene            7087..>9827
                     /locus_tag="VNN25_15105"
     CDS             7087..>9827
                     /locus_tag="VNN25_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH92910.1"
                     /translation="MFTSGFNRPARLLQAVAVLCFLAGTADAGVLGNRYLFVQPDASA
                     PASPKFGWVKVELTWAGQKAEAIPLSGAAAAGAEDVADYGSYRIVSVPEGSFKALGAQ
                     LAGQGIRVRAVDNFNRIDTPGASIDVRRGIDPATSPARLIHAYPPKTNGLYLVQFIGP
                     AKAEWYNALIDVGWSITRYLPANAYVVAGPPELTAGTAQLPFVQFFDFYHPFEKASVR
                     FRDGDPHDCLFEVPSTAGKNDAIDAISRLAAVGSVRVDSYENDTYIHARMRGNDAEAI
                     LSNPLVIGFGAEPVMGLSDERRTLSLTSNVNTTGSQPLNPTTYLSWLAGRCSLCTATN
                     MPYSTWRVGIADTGLDGGRFGVHHPDLTPREDWGSIFVSPNDPDPCGDCDNVTHGTMI
                     ASIIAGNATLGLTDPLGYYYGMGIAPMAGIFSTKISSLGHGMPNNIFAWAQDAASSSV
                     TIQNHSHNDYGFGETADPSGLYTTESRQYDQATRDADADSSNGRVPMLFTVSSGNTHF
                     GPYGNSTFTLPPATAKNVISMGGVENYRPDQDASACSGTQADDFRNIFFEGRHGTAIP
                     GYIKPDLVAPASMIVSARTQWNPQTNRGYCQENVDGNHMYEMDSGTSFAAPAASGAAI
                     LVKRYFGSSFPTDVSPAGAKAVLIAGAHSVRGGLDRTGNNQSTLNVGPAPNTQQGFGR
                     LSLDDVLTGATRPVLVDQTPQRHFTTSGQTRTTRLTVRDALKPVVVALVWTDAPATSE
                     TDPTHPVTPLVNDLDLTVFPLTTTCTYMQGNYINQTVNPSRGEESVSAPCAQVATPDT
                     VNNVEYARFFADNFTQFDVRVAGSMIQAPGDPGFSSNNNQDFALVVLNADLVSEGNIT
                     APQLTTTRDPVTPTTVHLSWTVPLNMIVDHYEIKRGSTASSLSTVYPNEHGTTKDDVG
                     "
BASE COUNT         1872 a         2956 c         2991 g         2008 t
ORIGIN      
        1 tgtggcacag acactcttgt ctgtgcgcct tacgcccgct gaacggctgg agaacagtcc
       61 gatcctgaac gtcctcatcg ctacgctgat gttctggttt ctgatcacgc agttccgcga
      121 caaaggcggg ctggccgcgc tcgacctcaa caacttcaac ttcgcgttcc tcgccgcggg
      181 actgctgctt cactggcgtc cgaggtcgtt tctgcgcgcg gtgacgaagt cagtgcctgc
      241 gacggccggg gttctgattc agttcccgtt ctacggcggc atcttcggga tgatctcgaa
      301 gacgccgatc gccgcgcatc tcgcacacgc gttcgtgagc gtgacgacga ggtcgacgtt
      361 cccggtcgtc gtcgccatct actccgccat cctcggcatg ttcgttccgt ccggcggcgg
      421 caagtgggtg gtcgaagcgc cgtacgtgat ggccgccgcg aatgcctggc acatgcacct
      481 cggatggacc gtgcagatct acaacgccgg cgaagcgcta ccgaacctga tcaatccctt
      541 ctggatgctc ccgctgatgg gcatcctcgg cgtccgcgcc cgcgacctcg ccggctactc
      601 gatcctccaa atgctcttcc acgtgccgat cgtgattctt ctttgctggc tgttcgcgca
      661 gacgctgtga atggcgagtg cggcgctggg tgtgaaggtg ccgacgatga aagtccgggg
      721 ccgtctcgta gccggccccg gtttactgcc actactacgc cgattacctc cggcgcttgt
      781 tgcacggtcg tggcgtgcgt gcgcacttcg cgaccgtcgc tcctggccgt ctcacgcgtc
      841 acgaaacctg gagacgctcg tgggcgcgtt caggccggcc atggagatga tgaagctggt
      901 caactacttg tttttcgcgt tgcgtcatct ccctcagccg accggcagct tcggttccat
      961 ttccacgaac gctcccggct ccggatcgcc ggccaactga tccttccctg ctttgaaggc
     1021 ttccttgact tccgctggct tgatggttgc cgtgacttcg tcgagcgctg tcaggaagac
     1081 gcgcagcgcg gcgcgccatg cgaccgggat cagcggaatt gccgcaaggc cgacaaggat
     1141 cggacgggcc acggcgtagg tcgtggacag acgatccgcc cggctggcct gcacccctcc
     1201 cggcgccgtg tgtgccgtca agtcgaatga ttgaatgtgg cggtcgatga tgtcgaaggt
     1261 gatgtgattc ttttggttgt gctcgtaacc agtgctcatg atgaatactc gtctttctgc
     1321 cgcgcgaagc gcgacgattg aaggttggtt gaaaggtttg ttgaaaggtt gaatagaacg
     1381 ttgaccatgg ccattactcc ttcgccgatg agccttcggg cagcaatgcc ttccggccga
     1441 agccggcaga ccgcagcaac gacgcaggca gagagagtcc cacccgcgaa aacgcatcga
     1501 agatcactct ttgcacgttg ccgccgatgc cgtacatctc gccggcgacc gtaaccgtgg
     1561 cctcggcgaa gtattggaaa tcggcctccg ggtgaagcct tgacgtcgcg gtgcggtacc
     1621 agatccgccc gccgatttcc caggcgtatc cacgaaggtc cgctgcagtg aaatagaaag
     1681 cgcggttcag gatccccgag ttgatgtgga cgccgccgtc atcgtcggga aagtccatgt
     1741 agcggcgcat gtgtgacggc tgcggatctt tgccgagcac aggatcgtcg taggcggtac
     1801 ccggcgcttt catcgagcgc agacctacgc cgcgcaccgc gggcccgaac acctccctgc
     1861 cgatcaccca atcggagcgc atagccgtta ggccgagccg ccactgcttg gtcaggattc
     1921 cgatcacatc ggagatgtgc tcgttcagtg cgcccggctg gccgcagtac aggagccctg
     1981 cgtcgtgctc cgtcataccg tgaatgtgct cgtgggcgac gacgtcgact ggggcggtga
     2041 aacggttgta caactggccg tctccgtcgc cgtagaccat ctgccgcccg ttccagaagg
     2101 cgttctcgaa atgcgtgccg tagtggacgg tcgagtcgag ccgcatgccg cggttgtcga
     2161 tggagtggcg caggaagaca ctcccgagga agtcgtacac ggctcctgag ccgtcgaacg
     2221 cctccctcat ttcgatatcg ctggcggtgg tccgctcctc ggtcatcacc agcgtgccgg
     2281 gcagcgtcag ttgaccgccg gcatggtaaa tgtggcgctt cttctcgagt ggccgctgta
     2341 cgcgcgggcc gatcagcgcg gcggcctgct cgcaggagcg ttgcgccacg gtgtgtgcca
     2401 gagttgcggc ggcgcgttcc cgctgcttgc tgttgccgtg ctgagccaca tgattctgga
     2461 tgtgtggagg gatgacacag caggtgcttt gcgttatgtt tcgcaagaca ctactccttt
     2521 ctctcaaggt ttggggttga tgtcgaaggc acggccgcgc gcaaacgcgg ccgtgctgtt
     2581 tttcacggaa tcgttagtcc ttgccacctc ctttcgccgt tggatcaggc gtgcctggat
     2641 ccgcttctgc cggcggcttc gcgggctgta cccgctttgg actcgccagc tcggccctga
     2701 ggttctccac gtgcactttc atgtgggtgt tgttggggtt caaggccacg cgctgggcga
     2761 tgttgtagat cgcaagcgcg ctttttcctg cccttgcttc cttcactcgt ctcatcaaat
     2821 cgagacgccg ggccgtgccg aagagctgat ctgtgagcga ctgaatacct tgcgaaaact
     2881 gcagcgtgtc ccggcactcc atcgtgtcga gctggttgac gccctgcagc tcggggctgg
     2941 actccacagc cgccaccgtc atcccaatga atttcgctgt cacccgacgg cgaaggttct
     3001 tgggagtctc ggacagatca tcgttgtagc cggggatctg cacgacggcg gcgttgatgg
     3061 cggcgccgag ttgttcagcg atcttctcgt acggcgtgat taccggatcc ggtacaggca
     3121 ccggctcggc gacagcactc tgctgtctgg aaatgcgctt cctcataggt tctccttcaa
     3181 gttagacggg ccagaatcta tgaggtttct ggaccgtttt ttggttaccc gggctccctc
     3241 gcgaagggaa cttacgggcg ttcggcattt agtcgacttg ccgcttatga acctaggccc
     3301 ggtcttatgg ggtgtcaagg gggggtatgc ctttagagca tagatttctt gaccatactt
     3361 cacgcaaata ggcagttaaa tggtttaacg gcattaccgc gacgcggctg gccggatgca
     3421 tttgagagca gcgaatgtgg gattgcgtgt gtgttgagcc ctcgtctgcg tgagacggat
     3481 ggtaattccc agaagttgtc cgtctaaggc cgcgcaatgt tgcattggtc cgatcgggtc
     3541 acagtcggcg gcgaatcctg gccataaaaa gcccgcaacg tgtcgttatc ggaggcatct
     3601 cgtcggcgcg aagagctcga cggcggcgcg gcagaacgat acgcaatgtc ggcgcagcgg
     3661 aaacagacac ctattagcgg cttttctccg gctcacgccc cctatagtgg tcgtttcatg
     3721 ccggaggtag ggaaaaccat gccagacgac actcctacac cggagccggg gaggaatcgc
     3781 gcgagtggat gggaaagagc acacaactcc gttggccgca gtcatggccg ctctcgggtt
     3841 gaagccgttt cacgtcatac agaccgcgaa ccgcatcgcg gcgcagatgg gacttcgcca
     3901 cgcgatcagc cgccagcact tcctcaagct gcggatcggc gagcggaacg caaccgagga
     3961 gaagattctg cttctggtga tggccttccg cgagctgagc ggctacgcgg tccgcgcgag
     4021 cgatcttttc gccctggagc cctccgccgc ggtgggggac gcgcctctcc tgacgccatg
     4081 tatttcggcg catggcagca acgcattggt tcctatatcc tcggcgggcg ctggttttcg
     4141 cgcccggagg atttccgtgt ctgaccgaac cggcacttcc ggtgacgacg agttcgaagc
     4201 gttgtacacc gagtacggct tactgctccg cagcatcgca atgcggagat acgggattcc
     4261 gcccgacgat gccgaggcgc tcacccacga cacgtttgtg gcctatctcc agcggcacac
     4321 caagatcaac gatctgaagc catggctgat gggtgcagtt tgcaatgcgt gcaagcacta
     4381 ctggcgcgat cgaaaacggg aaatgcccct tccggaggac ttcgaggaga cgatcgaccc
     4441 tacggcgaac tgctcggccg atagctgggc atggcggatc acggttgccg gtcttctggc
     4501 ccgtttggga aatcaatgcc gcgagacgtt gaagcgctac tactggtcgg atgaatcgaa
     4561 agaggcgatc gccgctacgc tttcgctatc gcccggctac gtgctgcatc tgttgtcgtc
     4621 atgcaggcag cgcatgcgcg agatgctgaa gaggaggact tcatgactca ccccgacgaa
     4681 gcggtactga gcgcattcag cctcgacgcg gagtctgtgg ccgatcgtga agttgtggcc
     4741 gcgcatgtcg cagactgcga ggcctgccag gaggctatcg cggcattccg gaagttcgaa
     4801 gacgcgctgc ggagcccgga gacctggatg caccttaccg ccttgcagtc tccaggcgac
     4861 cggtttgcgg aggctgaggc actgcgacgc cgagtgcaga aggaggacga cgaagcgtcg
     4921 cgcatcctcg cgtcgctcgt gaattccccg ctgcggttcc gaaacgcgaa gctcgaggag
     4981 aagccgggct gtcatacgga agggatggtg cgcatgctgt gcgcagccgc gaacgagcgc
     5041 cacgaaagcc gtccgacatt cagcctggaa ctgacggtga cggcgtgcac gatcgcgaag
     5101 gccctccccc agacaaacgg cgccgggcgg cggttgagca tgggcatggc ccttcgcgag
     5161 cgtgcaaacg cgctccgata cctcggcaag ttcaatgacg cgctactggc cctggagtac
     5221 gcggaacggt tcttcgatac gtcaccggga gcggatgact tcgacttggc catcgtctgg
     5281 tacatccgcg cggttgtgtt caacaagagc gatcggcttc cggaaggcat catcgcggca
     5341 cgggatgcgg cgcgagtatt tcgcgagtac ggagacaggg atcgggaact atcgtcgatc
     5401 atggtcgagg cgggctgtct cttgctctct ggaaatgcgc aagatgccgt ggaagccttc
     5461 gggcgcgtcg ccgacctcgc aagagagcgg agcaacgtgc ggctactcgc gagcgcgctg
     5521 cagaacggtg cggctgcctt ggccgacttg ggacagctgg agcgcgcgga gcgtttgtat
     5581 agcgaagcga tcgtgctcta tgacgagctg cgggtcccca ccgaacatgc acgagcggta
     5641 tgggcacttg gctccgtggc cgtcgcagct gggaatctcg aagaaggggc ggcgagactg
     5701 gatgcaagcc gaaaggagct tgcccatctg gggctgacaa acgatgctgc gcaggcgact
     5761 ttggagtggg cggaggcgcg tctcgcgctg aacaagcccg aaggcgtggc cgcggcctgc
     5821 cggaagatcg tgatcgtctt caactcagaa ggcatgcagc atcacgccaa ggaagccctc
     5881 gctaccctcc acgaagcgct ccgtgccggc aaagcgacgc ctgagctcgt ccgcagcgtc
     5941 cgcgcctacc tcgatcagtt gccggcgaac ccgacgcagc ccttcgtgat ggcgtcgtaa
     6001 taaaaaatct catagtttgc cgcgctggtg gcacctcttc ccgagggcgc ccttccaacc
     6061 gttgaccggc cctgtgccgg atagcacaag gagggtgtat gcaaaggatt cgcatttttg
     6121 tggccgtggt gtctctgttc gggatgctgg gcctcacagg cggtgccatc gcagcgcaga
     6181 agaagaacga taatccgcca gcacgagcca cgcagagcca cgcgcagaag gaaatgaagg
     6241 ccccgcgcaa aggctggatc gcgaagatcc tcgacgatct cgagaacaag ctctcgttcc
     6301 cgcctggcta gccgatccgc aaagccaccg ctgcttatcg cggggcacag tgcgtagttt
     6361 gagacacggc gaaccgatac gattctccgc gtggagatcg atcgcggaca gctccgggcc
     6421 atcgtgcgcc gttcgctcgc ccgttacagg ggtggttcat tcgcgctcgg tgactcaggc
     6481 ggcgcgagag gaatgctgtt ctttacggac gttgaagggc gggagttcct ctgcggcggg
     6541 ttgaacgtag acaatgcgca gccgctctgc gacgccttga atgcgatgct acggctcgta
     6601 gagaagtgag gccgtcggtg tcgacgcgcg tcaggttaag gggtggcgca tacgcgtttc
     6661 ctagcctcgg cgctttctct tgcgactcca ctccttccgc atcgtctctg cgatctcctc
     6721 cgcgctgcaa aactcggaga gatcgacacc gctgcgttga cacgcatcga tgatcccggc
     6781 gatcagatcg gagcggctga tccagaagcc ccttttgaaa cgcatgtcaa tcgcgagccg
     6841 gtccaagaat gcgcgatgac gcacaagcag gttgagcgtc acctttgact gagaatcggc
     6901 ggcacgttgc tgcggtccgc gatcggcgaa tgaactcata tactgatcga catcctaaat
     6961 gatcgatgga ttgtcaggaa gctttcctgc gccccgtgca gcaccttctg ctcatttccg
     7021 ccaataacct ggcgtgacgg cagaggttta ctaacgtcag aatggcatgc tacattacat
     7081 gcattcatgt tcacttccgg ttttaacagg ccagctcggc tactccaggc tgtcgcggtt
     7141 ctgtgctttc tggcgggaac cgcggacgca ggtgtgctcg gaaacaggta cctgttcgtg
     7201 cagccggacg cgtcagcacc ggcttctcca aagtttggct gggtcaaagt cgaactgact
     7261 tgggccgggc agaaggcaga ggcaatcccc ctttccggcg cggctgcggc gggggcagaa
     7321 gacgttgccg actatggaag ttaccgcatc gtgtcggtgc ccgaggggtc tttcaaggct
     7381 ctcggtgcac aactggccgg acagggtatt cgtgtccgcg cggtcgacaa cttcaatcgg
     7441 atcgacacac ccggagcttc gattgatgtc cgccgcggca ttgatccggc cacctcacct
     7501 gcgcggctca ttcatgcgta cccgccgaaa accaacgggc tctatttggt gcaattcatc
     7561 ggtccggcca aggccgagtg gtacaacgcc ttgatcgatg ttggctggtc gataacccga
     7621 taccttccgg ccaacgcgta cgtcgtcgca ggcccgccgg agctcacggc tggcaccgcg
     7681 cagctcccgt tcgtgcagtt cttcgatttt tatcatccgt tcgagaaggc gtcggtccgc
     7741 ttccgtgacg gcgaccctca tgactgtctt ttcgaggtgc cgtcgactgc aggaaagaac
     7801 gatgcaatcg atgcgatcag ccgtttggcc gccgttggct ccgtgcgggt cgacagttat
     7861 gagaacgaca cgtacattca cgcacgaatg agaggcaacg atgcggaggc aatcctgtcc
     7921 aacccgctcg tgatcggttt tggcgccgag ccggtgatgg gactctcgga tgagaggcgc
     7981 accttgtcgc tgacttcgaa cgtgaacacg accggatcac agccgctgaa cccgaccacg
     8041 tacctttctt ggctggccgg tcgctgcagc ctctgcacag cgacgaacat gccatattcg
     8101 acctggcgcg ttgggattgc cgacaccggt cttgacggtg ggaggtttgg cgttcaccat
     8161 cctgacctca ctccgcggga ggactggggc agtatcttcg tgtcgccgaa cgaccccgat
     8221 ccatgcggcg attgcgacaa tgttacacac ggtacgatga ttgccagcat cattgccggc
     8281 aacgccaccc tcggccttac ggacccgctc ggctactact acggaatggg tatcgcgccg
     8341 atggccggta ttttctcgac aaagatttct tcattgggcc acgggatgcc taataatatt
     8401 tttgcatggg ctcaggatgc cgcgtccagc tccgtgacca ttcagaatca ttcgcacaac
     8461 gactacggct ttggagagac agcagatcca agcggtctgt atacgacgga gtcgcgacag
     8521 tatgatcaag ctacgcgtga tgccgatgcc gattcgagca atggccgagt gcccatgctc
     8581 ttcaccgtct cctctgggaa cacgcatttc ggcccttatg gcaactccac gttcacactt
     8641 ccgccagcga ctgcgaagaa cgtcatttcc atgggcggag tcgaaaacta caggcccgac
     8701 caggacgcca gcgcttgtag cggtacccag gccgatgact ttcgcaatat cttcttcgag
     8761 ggcaggcacg ggacagccat accggggtat atcaaacctg atcttgttgc gccagcctcg
     8821 atgatcgtct cagcgagaac tcaatggaat ccgcagacga accgcggcta ttgtcaggag
     8881 aacgtagacg gcaatcacat gtacgaaatg gatagcggca ccagttttgc tgcccccgcc
     8941 gcatccggcg cggctattct cgtaaaacgg tattttggct cctcctttcc cacagatgtc
     9001 tctccagcag gagcgaaagc ggttctaatc gcgggtgcac acagcgtacg gggcgggtta
     9061 gatcgcactg gcaacaacca gtccacgctt aacgtcggcc cagcaccgaa cacgcagcaa
     9121 ggattcgggc ggctgtcgct cgacgatgtc ctcaccggag cgacacgtcc agtcttggtg
     9181 gaccagacac cccagcgaca tttcaccacc tccggccaga cccgaacgac ccgtctgacc
     9241 gtgcgcgatg cgttgaagcc ggtcgtggta gcgctcgtat ggaccgacgc accggcgacc
     9301 tcggaaacag atccgaccca ccctgtcact cctcttgtca acgatctcga tctcaccgtc
     9361 ttcccgctca cgacgacttg cacatatatg caaggaaatt acatcaacca aaccgtcaac
     9421 ccgagccgag gggaggagtc cgtctccgct ccatgtgcgc aagtggctac tcccgacacc
     9481 gtcaacaatg ttgaatatgc gcgcttcttc gccgacaact tcacgcagtt tgacgtgaga
     9541 gtagctggct cgatgattca agcgccaggc gacccggggt tttcgtcaaa caacaaccag
     9601 gatttcgccc tagttgtctt gaatgcggat cttgtgagtg aaggaaacat cactgctccc
     9661 caactcacga ccacccgcga ccccgtaacg ccaacaacgg ttcatctgag ctggacggtg
     9721 cccctcaaca tgattgtcga tcactacgag atcaaacgtg gatcgacggc ttcgagtctc
     9781 agcacagtgt atccaaacga gcatgggaca acgaaggacg acgtggg
//
LOCUS       DATLVW010000503         9684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_104261, whole genome shotgun sequence.
ACCESSION   DATLVW010000503 DATLVW010000000
VERSION     DATLVW010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104261"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VNN25_15110"
     CDS             <1..201
                     /locus_tag="VNN25_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HXH92911.1"
                     /translation="DFAKSRDAVRDLATELLTIEATGDYPRAKTLLDRYGKETPEMAA
                     VNATLKDIPVDITPVFPAAGEK"
     gene            complement(280..2184)
                     /locus_tag="VNN25_15115"
     CDS             complement(280..2184)
                     /locus_tag="VNN25_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXH92912.1"
                     /translation="MAEVKSQGAVDAMDVAEGARETEWEKPSFVGDLFLGKLNLDLIH
                     PFPEQPEADRAEADEFLGRLERFLTESVDADKIDQQGEYDYSLFDGLNQLGAWGMKIP
                     KEYGGLGFSATNYNRAIGLVATWCGSTVAWLSAHQSIGVPQPLKLFGTPEQKKKYLPR
                     LANGAISAFALTEPGVGSDPAKMETTAVKSPDGKGWIINGEKLWCTNATKAELLVVMT
                     RTAPKIVKGKEKKQITAFIVETNSPGFSIEHRCQFMGLRGIGNALIKFDNVFVPNENL
                     LWGEGLGLKLALITLNTGRLTIPAGCAAAGRKMVDIAKDWANERVQWGAPIGKHEAVA
                     AKIAWMASHTFAMEAIVWLTSGLADRGNVDLRLEAAMAKLFNTEVAWQISDHLVQIRG
                     GRGYETAQSLRARGEKAYPVERIMRDMRINRIFEGTSEIQHLFIAREAVDPHMKRGFK
                     ILQPETPMGEKLGAAAKAGLHYAGWYPSRYVGWSRFPKFGEFGKLTGHMGYIERTSRH
                     LSRSIFHSMMRFQAKLERKQMVLFRIVDIGTDLFAMAAAISYATMLAKKGGAEKKNAV
                     DLADVFCREARMRIDANFDNLFTDHDDAAYKLVMHLLKGEFDWFKGQVVDDPVMPTPE
                     QVEEAMAKLA"
     gene            2338..3015
                     /locus_tag="VNN25_15120"
     CDS             2338..3015
                     /locus_tag="VNN25_15120"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HXH92913.1"
                     /translation="MKNRTPAELVVAPEVEAIIEDRLLALPRASVDREQLQHHLREVL
                     KLLHVDMEDENIRDTPRRWAESLVTMTSGYDYTDLKKLTTLFRKACSSADEQCQNLVV
                     IGGTYKTLCAHHILPFFGHFIVGYIPEKMIIGASKIPRIVEVHMRRLQSQEHLAHDVA
                     DTIERILEPRGIAVWMGGVHLCMVMRGVEQESSFMETNVLRGEFRNDERTREEFMSIV
                     NRRGQRG"
     gene            complement(3095..3835)
                     /locus_tag="VNN25_15125"
     CDS             complement(3095..3835)
                     /locus_tag="VNN25_15125"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXH92914.1"
                     /translation="MKKLLIALTVAATSVIAFAQAPADDAPHRHGHRGAFHQLSAKLN
                     LTDTQKQQMKDIRSADRASNQQLYADFGSKLHQLRALRQANDPGAAALKAEIESMKPQ
                     VKAARKASRQAMLAVLTPDQRAQLKELRQSQGFGRLRANVVRHAVASKLNLTNEQKSQ
                     IQQLRKATQDKNQQLFADAKAKREEFHSLKKANDPRAAEVKAELEALRPQLQAAKQEQ
                     HAAFLNILTPEQRSQLEQWKSRREQQLR"
     gene            3993..4655
                     /gene="lepB"
                     /locus_tag="VNN25_15130"
     CDS             3993..4655
                     /gene="lepB"
                     /locus_tag="VNN25_15130"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXH92915.1"
                     /translation="MDKKKVFYEVRVFLLMLLVISSLRSALADWNDVPTGSMKPTIEE
                     GDRVVVNKLAYDLKVPFTMIELLKWSDPKRGDIVVLFSPADGVRLVKRVVALPGDKIE
                     MRENQLLVNGQAAVWKPVAQRDDDEQGASFIVEETMGGRTHDVMFTPQIPATRSFGPL
                     VVPAGRYFVMGDNRDNSNDSRFIGMIERRRIVGKAMAVAFSLDRNHWYRPRFHRFFKG
                     IQ"
     gene            complement(4840..5121)
                     /locus_tag="VNN25_15135"
     CDS             complement(4840..5121)
                     /locus_tag="VNN25_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH92916.1"
                     /translation="MKSAVPPGTKPSVQVTTGVAAPVPEAPLESARRITREEAIKLVK
                     EKKAVYVDVRPKESYDQAHITGAMNIPLGDVITRVKELPPGKFIITYCA"
     gene            5316..5696
                     /locus_tag="VNN25_15140"
     CDS             5316..5696
                     /locus_tag="VNN25_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92917.1"
                     /translation="MRDHVRILAWCFIVYSSIIVLVGLAIGSVVLFGGAISGDREAML
                     ITGAVGAGIACFLVVISLPGMFAGIGLLKMQPWARIVAIIVGVLHILSFPLGTALGVY
                     TLWVLLNAETEAMFRGQQQVAVAV"
     gene            complement(5758..6114)
                     /locus_tag="VNN25_15145"
     CDS             complement(5758..6114)
                     /locus_tag="VNN25_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92918.1"
                     /translation="MKRSIAMVLFVAMAGTLALAATKPRFDAKLPALGTTFHSLPAGK
                     GKSLVEGSCLRCHSADMLAQQRLTEKQWTAEVDKMMRWGAVVKDRDKPMMLAYLVKHF
                     GPENKFAPIRTRPSGF"
     gene            complement(6111..7244)
                     /locus_tag="VNN25_15150"
     CDS             complement(6111..7244)
                     /locus_tag="VNN25_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HXH92919.1"
                     /translation="MSFDRIDRRRFFLLAAAPLLTAQKAAVSHAREALLQLNGYAVNA
                     ETPLELLTEYLTPNELFFVRSHWIPRTPDPKRWRLTIDGEVDRSQQFTLSELKKLPRT
                     EVTCVLQCAGNGRGLYSPPLPGVQWRYGAAGNARWTGVRVRDVLDRAGLKSSAKHLHA
                     FGSDVPPDKVPPFHRSIEIEKVLADGILAFAMNGEALPVAHGAPLRLIVPGWAGDHWM
                     KWLVRLSPQVEPQKGFYMETAYRYPLAPGAAGVTFKPEEMKPVKELFVKSNITTAPKR
                     ARAGQVHEIRGFAFSGAPDIAKVDISDDDGATWQEATLDQRHDPYAWRLWSYRWTPAR
                     PGTTRLCARATDNHGNIQPREAVWNQSGYLHNSWQFVDVEVTA"
     gene            7328..8116
                     /locus_tag="VNN25_15155"
     CDS             7328..8116
                     /locus_tag="VNN25_15155"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH92920.1"
                     /translation="MKQTRSGADYSWTPSRDFREICACQSHARRERTLWRRALRLARR
                     RGVALLVTVPLAFGAIGIEAMNLSLPALAQEAIGHRHFPVFTTRKVREAFLKPEQAAT
                     TLTLGIAKEEFFRTEVPFGSIIYREALKNDLAPELIAAIVESESDFRPHLVSNKNAQG
                     LMQIVPETSRLMGCNDPFDPYNNVAAGTKYFRYLLNRFGDERLALAAYNAGEGTVERL
                     GDVPEYPETIEYINRVAARTISYRNRFQHRYEASTQLQASVLPH"
     gene            complement(8128..>9684)
                     /locus_tag="VNN25_15160"
     CDS             complement(8128..>9684)
                     /locus_tag="VNN25_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXH92921.1"
                     /translation="RQMAAEVFLTRLFEKYQSYGEALQAASALDVAIQDSIDERRAKI
                     RDTEKLSSSLTIILTLLALTSAMLVAGLGRQMRLLAREAMGRRHEAEREAAEAQLARA
                     GSEREERRAAFLAAAGRELATTLDYEHAIATLAKLIVPNIAEVCVIDLAEADGALRRA
                     AVAHRDPELERALMTRAGQIVREIPGPLAQVIQERETRVIGYHSSLLDYVGVSSEEPR
                     SLLAVPLVSRGQSLGIILAASAEAKLFTPGDAALVGELSSHGSLAIDNARLYLESQQA
                     VRAREEMLAIVSHDLRNPLHAVMLSISMLQTAGTIDDDDREQLEIIDISAKRMQRLIE
                     DLLDVTRLEGGKQLPIEPAPLDVESLFAETQELFKSQAATNAITLQYNVAAGLKTVLA
                     DRHRVLQVLSNLIGNAMKFTPGGGMITYRAEPQNENAVKITVADTGPGIPKENLGDIF
                     NPYWQAKRTARMGAGLGLPIAKGIVESHGGRIWVESAPGAGTKFFFTLPVAQSVEPVD
                     VAQSEASASR"
BASE COUNT         1740 a         2949 c         3003 g         1992 t
ORIGIN      
        1 gatttcgcga agtcgcgcga cgccgtccgc gatcttgcca ctgagctcct gacgatcgaa
       61 gcgaccggcg actacccgcg cgcaaagacg ttgctcgacc gctacggcaa ggaaacgccg
      121 gagatggcag cagtcaacgc aacgctgaag gacattccgg tcgacatcac tccagtcttc
      181 cccgcggcgg gagaaaagta gcgagggaca gcaccagacg acgagaaaaa gattcggggc
      241 ggctaccagc cgccccgatc aatcgccatc agagtctatt tacgccagct tcgccatcgc
      301 ctcttctacc tgctccggcg tcggcatgac cgggtcatcg acgacctggc ctttgaacca
      361 gtcgaactcg cctttgagca ggtgcatgac gagcttgtag gcggcatcgt cgtgatcggt
      421 gaagagattg tcgaagttcg cgtcgatgcg catgcgggct tcgcggcaga agacgtcggc
      481 gagatcgacc gcgtttttct tctcggcgcc gcccttcttg gccagcatcg tcgcgtagct
      541 gatcgccgcg gccatggcga agaggtcggt accgatgtcg acgatgcgga acagaaccat
      601 ctgtttgcgc tccagcttgg cctggaagcg catcatggag tggaagatcg agcgggaaag
      661 gtgccgtgag gtgcgctcga tgtagcccat gtgacccgtc agcttgccga actcgccaaa
      721 cttcgggaaa cgtgaccagc cgacgtagcg cgacgggtac cagcccgcgt aatggaggcc
      781 agccttcgcg gctgctccga gcttctcgcc catcggcgtc tcgggttgga gaatcttgaa
      841 gccgcgcttc atgtgcggat ctacggcctc acgggcgatg aagagatgct ggatctcgct
      901 cgtaccttcg aagatgcggt tgatgcgcat gtcgcgcatg atccgctcga ccgggtaagc
      961 cttctcgccg cgcgcgcgga gcgactgcgc ggtctcgtag cctcgcccgc cgcggatctg
     1021 cacgaggtga tcgctgatct gccaggccac ttccgtgttg aagagtttcg ccatcgccgc
     1081 ttcgaggcgg agatcgacgt tgccgcggtc ggccagaccg gacgtaagcc acacgatcgc
     1141 ttccatcgcg aatgtgtgcg aggccatcca ggcgatcttc gccgccaccg cttcgtgctt
     1201 gccgatcggc gcgccccatt gcacgcgctc gttcgcccag tctttggcga tgtcgaccat
     1261 cttccgccct gcggcggcgc atccagccgg aatcgtgaga cggccggtgt tcagggtgat
     1321 cagggcgagc ttgagtccga gaccttcgcc ccagaggagg ttctcgttcg ggacgaagac
     1381 gttgtcgaac ttgatgaggg cgttgccgat gccgcgcaag cccatgaact ggcagcgatg
     1441 ctcgatcgag aagccggggc tgttcgtctc gacgatgaac gccgtgatct gcttcttctc
     1501 tttgcctttg acgatctttg gcgctgtccg cgtcatcacc acgaggagct cggccttcgt
     1561 ggcgttggtg caccagagtt tctcgccgtt gatgatccag cctttgccgt ccggcgactt
     1621 gacggcggtc gtctccatct tggccggatc agaaccgacg cccggctcgg tgagcgcgaa
     1681 tgcggagatc gcaccgtttg cgaggcgcgg caggtacttc ttcttctgct cgggagttcc
     1741 gaagagtttg agcggctgcg gaacgccgat ggactggtgt gccgaaagcc aggccacggt
     1801 cgatccgcac cacgtggcca cgaggccgat ggctcggttg tagttcgtgg cgctgaaccc
     1861 gaggccaccg tattctttcg ggatcttcat gccccaggcg ccgagctgat tgagtccatc
     1921 gaaaagcgag tagtcgtact cgccttgctg atcgatctta tcggcgtcga cgctctcagt
     1981 gaggaagcgc tccagccggc cgaggaattc gtccgcttcc gcgcggtccg cttccggctg
     2041 ttcggggaac ggatggatca ggtcgaggtt gagtttgccg aggaaaagat cgccgacgaa
     2101 cgacggcttt tcccactcgg tctcgcgcgc accttcggcg acatccatcg catcgacggc
     2161 accttgtgat ttgacttcgg ccatttggaa actccttcgg atggggctga ttacacaaat
     2221 cgttccgccg cggtggctga gcgtcgatta gtatgaatga acgctcattc agttgtcaac
     2281 cgagtggttc tacaattgct tagttgcgga ttttgaatca cgtacacttc cgccgccatg
     2341 aagaaccgca ctcctgccga acttgtcgta gctcccgagg tggaagccat catcgaggat
     2401 cgcctgctgg cgcttccacg agcctccgtc gatcgcgaac agctccagca tcacctccgc
     2461 gaagtgctca agctgctcca tgtcgatatg gaggacgaga acatccgcga caccccgcgt
     2521 cgatgggccg agtcgctggt gacgatgacg agcggctatg actacaccga tctgaaaaag
     2581 ctcacaaccc tcttccgcaa agcctgttcc tcggcggacg agcagtgcca gaacctggtg
     2641 gtgatcggtg ggacgtacaa gacgctctgc gcccaccaca tcctgccctt cttcggacat
     2701 ttcatcgtcg gctacatccc ggagaagatg atcatcggcg cgtcgaagat cccacgcatc
     2761 gtggaggttc acatgcgccg gctgcagtcg caggagcacc tggcgcacga cgtggccgac
     2821 acgatcgaac ggatcctcga gccgcgcggg atcgccgtat ggatgggcgg cgtgcatctc
     2881 tgcatggtca tgcgcggcgt ggagcaggag tcgtcgttca tggagaccaa cgtcctgcgc
     2941 ggcgagttcc gcaacgacga acgaacaagg gaggagttca tgtcgatcgt gaaccgccga
     3001 gggcagcgag ggtaggaact gcgcaggagc ccccaaaaaa tgcgccgccg gattgctccg
     3061 gcggcgctga gggagggggg gaggtactac cgagtcagcg gagctgctgt tcgcgccggc
     3121 tcttccactg ctcgagctga gaacgctgct cgggcgtgag aatgttgagg aatgctgcgt
     3181 gctgctcctg tttcgcagcc tggagttgcg gacgaagcgc ttctagctcc gctttgactt
     3241 cggcggcgcg cgggtcgttc gccttcttca gcgagtgaaa ctcttcgcgc ttggccttcg
     3301 catcggcaaa gagctgctga ttcttgtcct gcgtggcttt gcgaagctgt tggatctggc
     3361 ttttctgctc gttggtcagg ttcagtttcg atgcgacggc atgacggacg acgttggcgc
     3421 ggaggcggcc aaagccctgc gactgccgga gctctttgag ctgggcgcgc tggtcagggg
     3481 tgagcactgc gagcatggcc tgacggctgg ctttgcgcgc cgccttaacc tgcggcttca
     3541 tcgactcgat ctctgctttc agcgctgccg ctcctggatc gtttgcctgc cgcagcgcgc
     3601 ggagctggtg caacttcgag ccgaaatcgg cgtagagctg ctggttgctc gcgcggtcgg
     3661 cggagcggat gtctttcatt tgctgtttct gggtatcggt gagattgagt ttcgcgctga
     3721 gctgatggaa ggcgccgcgg tgtccgtggc gatggggcgc gtcgtctgcg ggcgcctggg
     3781 cgaaggcgat caccgacgtg gccgcgactg tcaatgcaat gagaagcttt ttcatgacgt
     3841 tcatgtcctc cgtggatgtg atgctgttca ctactacgat gaagcctgcg aggcgttgaa
     3901 acgcccgttg cttcgctctc taacaataat cttcaaagcc tgcagggacc cgcagatcag
     3961 acggcctcgg ccggaaagtg cgtacagaac cgatggataa gaaaaaagtc ttttacgaag
     4021 tacgggtctt cctcctgatg ttgctggtca tcagctccct ccgctcggcc ctggctgact
     4081 ggaatgatgt gccgaccgga tcgatgaagc cgacgatcga ggagggcgac cgcgtcgtgg
     4141 tcaacaagct tgcctacgat ctgaaggtgc cgttcacgat gatcgagctg ctgaagtgga
     4201 gcgatccaaa gcggggcgac atcgtcgtcc tcttctcacc ggccgacgga gtccggctgg
     4261 tgaagcgagt cgttgcactg cctggcgaca agatcgaaat gcgcgagaac cagcttctgg
     4321 tgaacggtca ggccgcggtc tggaagccgg tggcccagcg cgacgatgac gagcagggag
     4381 cctcattcat cgtcgaggag accatgggtg gaaggacgca cgacgtgatg ttcacgccgc
     4441 agatcccggc cacgcgctcg ttcgggccgc tggtcgttcc ggccggccgc tatttcgtga
     4501 tgggcgacaa tcgcgacaac agcaacgaca gccgcttcat cggaatgatc gagcgccggc
     4561 gcatcgtcgg gaaagcaatg gccgtggcgt tctcgctcga tcggaaccat tggtaccgtc
     4621 cgcggtttca ccgcttcttc aaaggaatcc agtaggcgcg tgcgggcccg gcgagcaccg
     4681 ggccctctcg agattgcgtt actttttcag tacggtcgtg ccttcggttg ggagttgggc
     4741 tgccgtccag ccgttccatc cgccgaggag cgcggcagtg ttcttgattc cgtgagcatt
     4801 gaggtccagc accgcacggg cgcttgtatg ctcgttctgt caagcacaat acgtgatgat
     4861 gaactttcct ggaggcagct ccttgactcg ggtgatcacg tcaccgaggg ggatgttcat
     4921 cgcgcctgtg atgtgcgcct ggtcatacga ctctttcggc cggacatcga cataaacagc
     4981 tttcttttcc ttgacgagct tgatcgcttc ttcgcgagtg atccggcgcg cggactccag
     5041 cggtgcctcc ggcacaggag cagccacccc ggttgtaacc tgcaccgatg gcttcgttcc
     5101 tggcgggaca gccgacttca tctgagcttg agccagacct gtcgcgagaa aggcgaccac
     5161 ggctgttgca gttacaaaat gacgcatctc ttgccgagcc tccttattga gaattctact
     5221 ttttgcgttc gttgacgcag acttcggaaa cctaagcgaa gggtagcatg ttcccagcgc
     5281 aaccctgcgt gacactctgc gtaagattca tcatcatgag agaccacgtt cgcattctgg
     5341 cttggtgctt catcgtctac agctcgatca tcgttctggt cgggctggca atcggcagcg
     5401 tcgtcctgtt cggcggagcg atctcaggtg accgtgaggc gatgctcatc accggcgcgg
     5461 tcggcgccgg catcgcatgc ttcctcgttg tcatctcgtt gcctgggatg ttcgccggta
     5521 tcggtctgct gaagatgcag ccatgggcgc ggatcgtcgc gatcatcgtc ggcgtgctgc
     5581 acattctcag tttcccgttg ggaacggccc tcggcgttta cacgctgtgg gtgcttctca
     5641 atgccgagac agaggcgatg ttccgggggc agcagcaggt agccgtggcg gtctaagcgt
     5701 tttcacgcgg aggcgcggag ggcgcggagc catcgctcca tgtgagacct gaaatcgtca
     5761 aaagcccgac ggccgcgtcc tgattggcgc gaacttgttc tcgggcccga agtgtttcac
     5821 cagataagcg agcatcatcg gcttgtcgcg atccttcacg acggcacccc agcgcatcat
     5881 cttgtcgact tcggccgtcc actgcttctc cgtcagccgc tgctgcgcaa gcatgtccgc
     5941 tgagtgacat cgaaggcagc ttccctcgac gaggctcttt cccttgccgg ccggcaacga
     6001 gtgaaaagtc gttcccagcg ccgggagttt cgcgtcgaaa cgcggcttcg ttgccgcgag
     6061 cgcgagcgtt ccggccatcg ccacgaacaa aaccatcgcg atcgatcgct tcacgccgtc
     6121 acctcgacgt cgacgaactg ccagctattg tgaaggtatc cgctctgatt ccagacagcc
     6181 tcccgcggct ggatgtttcc gtggttgtcg gttgctcgag cgcaaaggcg tgtcgtgccc
     6241 gggcgcgcgg gagtccatcg atacgaccac aaccgccagg cgtagggatc gtggcgctgg
     6301 tcgagcgtcg cctcctgcca cgttgcaccg tcgtcatcgc tgatgtcgac tttcgcgatg
     6361 tcgggcgctc ccgaaaaggc gaagccccgg atctcgtgca cctgtccggc ccgcgccctc
     6421 ttcggtgctg tcgtgatgtt cgacttcacg aatagctcct tgaccggctt catctcctcg
     6481 ggtttgaacg tcacgcccgc ggcgccaggg gcgagcggat agcgatacgc cgtctccatg
     6541 tagaagcctt tctgcggctc cacttgagga gagagacgaa ccagccattt catccaatga
     6601 tcgcctgccc agccagggac gatcagtcga agcggcgcgc catgcgcgac cggcagcgct
     6661 tctccattca tcgcgaaagc gaggatcccg tcggcgagga ctttctcgat ctcaatgctc
     6721 cggtgaaacg gcggcacctt gtccggcgga acatcgctgc cgaatgcgtg cagatgcttc
     6781 gcgctgcttt tgagtcccgc gcggtcgaga acatcacgta cgcgcacgcc ggtccatcgc
     6841 gcgttgccgg ccgcaccgta gcgccattgc acgccgggaa gaggcggtga atagagcccg
     6901 cggccgttgc cggcgcactg aaggacgcag gtcacttcgg tgcgcgggag ttttttcagc
     6961 tcggaaagtg taaactgctg tgagcggtcg acctcaccgt cgatcgtcaa cctccacctc
     7021 ttggggtcgg gcgtgcgcgg gatccagtgg ctgcggacga agaaaagctc gttcggagtc
     7081 aggtactcag tgagcagctc gagcggcgtt tccgcattca cggcatagcc attcaactgc
     7141 agcaacgctt cccgagcgtg agaaaccgcg gctttctggg ccgtgagcaa cggggccgcg
     7201 gcgagtagaa agaaacgccg gcggtcgatg cggtcgaacg acatgccaga agtgtaaccc
     7261 actacgaatg aacgacttga tttcaacagc ctcgaacgtg gtccgactct tgccatcgcg
     7321 atgcttcatg aagcagacgc gatctggtgc tgactactcc tggacgcctt cacgcgactt
     7381 tcgcgagatc tgtgcgtgtc agagtcacgc gcgccgcgag cgcacgctct ggcgccgcgc
     7441 cctccgcctt gccagaaggc gcggggtggc gttgctcgta acggtgccgc tcgcattcgg
     7501 tgccatcggc atcgaagcga tgaatctttc gttgcctgca cttgcccagg aggcgatcgg
     7561 ccatcgtcat ttccccgttt tcacgacgcg caaagtgcgt gaagcgtttc tgaaaccgga
     7621 gcaagccgcc accacgctga ccctcggcat cgcaaaagaa gagttcttcc gaacggaagt
     7681 gccgtttggt tcgatcatct atcgcgaagc gctgaagaac gatctggctc ctgagttgat
     7741 cgcggccatc gtcgaatcgg aatccgattt tcgtccgcac ctcgtatcga acaagaacgc
     7801 gcagggcttg atgcagatcg tgccggagac cagccggctc atgggctgca acgatccctt
     7861 cgatccgtac aacaatgtgg ctgccgggac gaagtacttc cgctacctgc tcaatcgttt
     7921 cggcgatgaa cgcctcgcgc tggctgccta caacgccggc gaagggacgg tcgaacggct
     7981 cggagacgtt ccggagtacc cggaaacgat cgaatacatt aaccgcgtcg ccgcccgcac
     8041 gatctcgtac cgcaatcgct tccagcatcg atacgaagcc tccacgcagt tgcaggcgag
     8101 cgttctcccg cactaagcgg ctgcggacta ccggcttgct gatgcttcgg attgcgcaac
     8161 gtcgaccggc tcgaccgatt gcgccacagg cagtgtgaag aaaaacttcg tgcctgcacc
     8221 gggcgcgctc tccacccaga ttcggccgcc gtgcgattcg acgatccctt ttgcgatcgg
     8281 cagccccagt ccggcaccca tcctcgcggt gcgcttggct tgccagtaag gattgaaaat
     8341 atcgccgagg ttttctttgg ggatgccggg tccggtgtcg gcgacggtga tctttaccgc
     8401 gttctcattc tgaggctcag cgcgatacgt gatcattccg ccgccaggtg tgaacttcat
     8461 cgcgtttccg atcagattcg aaagcacctg caacacgcga tggcgatccg caaggacggt
     8521 cttcaaacca gccgcgacgt tgtattgcag cgtgatcgca ttcgtcgccg cttgcgattt
     8581 gaagagctcc tgggtctcgg cgaagagcga ctcgacgtcg agcggcgccg gctcgatcgg
     8641 caattgtttg cctccttcga gccgcgtgac gtcgagcaga tcctcgatca ggcgctgcat
     8701 ccgtttcgct gagatgtcga tgatctcgag ctgctcgcgg tcgtcgtcgt cgatcgttcc
     8761 ggcggtctgc aacatcgaaa tcgacagcat cacggcgtgt agcggattgc gcagatcgtg
     8821 cgacacgatg gccagcattt cctcgcgcgc acgcacggcc tgttgcgact cgaggtaaag
     8881 ccgggcgtta tcgatggcca gagagccatg gctggacagt tcgccgacca gtgccgcgtc
     8941 tcctggtgtg aagagctttg cttccgcgga cgccgcgagg atgattccga gcgattgtcc
     9001 gcggctcacc agaggcactg cgagcagcga ccgcggctct tccgacgaga cgcccacgta
     9061 gtcgagcaat gatgagtggt agccgatgac acgcgtctca cgttcctgaa tcacctgggc
     9121 cagcggtccg ggaatctcgc gaacgatctg accggcgcgc gtcatcaggg cgcgctccag
     9181 ctcgggatcg cgatgcgcaa ccgcggcgcg gcgaagcgca ccgtccgcct ccgccagatc
     9241 aatcacgcag acctcggcga tattcggcac gatgagtttc gcaagtgtgg cgatggcgtg
     9301 ctcgtagtcg agcgtcgtgg ccagctcgcg tccagcagca gccaggaacg cggcccggcg
     9361 ctcctcccgc tcggacccgg cacgggcaag ctgcgcttct gctgcctccc gctccgcttc
     9421 gtgcctgcgc cccatcgctt cgcgggcgag aagcctcatc tgccgtccga ggcccgcaac
     9481 cagcattgcc gacgtcagcg cgaggagcgt gaggatgatt gttagcgagg agctcagctt
     9541 ttcggtgtcc cgtattttgg cccgccgctc atcgatcgag tcctggatcg cgacatcgag
     9601 cgcggacgct gcctgaagcg cttctccgta cgattggtac ttctcgaata ggcgcgtcaa
     9661 gaagacttcg gcggccatct gccg
//
LOCUS       DATLVW010000504         3699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_20457, whole genome shotgun sequence.
ACCESSION   DATLVW010000504 DATLVW010000000
VERSION     DATLVW010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20457"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(543..1310)
                     /locus_tag="VNN25_15165"
     CDS             complement(543..1310)
                     /locus_tag="VNN25_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH92922.1"
                     /translation="MNVTTFLAMLGRDAHVARRNSVQLAFQTLLQPMMFVFVFGRVMT
                     SSGLMSAEYKSLLLPGIIALSMIMTGTWAVTMPLIAEFQFTKEIEDRLLAPMDLEWLA
                     VGKVVAGMIQALVSGLVVIPLGWLVMGGVNMSGAKLGLFILQALLVAMLSATLGLVLG
                     CSIGQQQVGLMFSMVMAPMIFFGCTYYPWIALAKFPILKRVVLVNPLVYASEGFRSAL
                     VPQAPHLPQAVILAALVAFDTGLLLFGLRQFRRKALT"
     gene            complement(1307..2266)
                     /locus_tag="VNN25_15170"
     CDS             complement(1307..2266)
                     /locus_tag="VNN25_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH92923.1"
                     /translation="MPIGIRTEGLRKVYPAAQQRGDVVAVDGLTLEVRPGEIFGLLGP
                     NGAGKSTTVGIVTTRVQPTAGRAWVGDLDVWKNSKAVKRLIGVVPQRPNLDFALTARE
                     ILTFHGAYFGLSSEERRTRADSLLEQFKLTDRANQKPLGFSGGMMQRLSIARAMMHDP
                     DVLFLDEPSAGLDPQTRLLLSEMIRGYNARGKTILLTTHNMEEADSLCQRVAIVDHGK
                     VIAVGTPKELKQSIPGGYLVRLQVGTTPAGFAEAVRLLQGVSEVREPRDGQLDVYADH
                     AGSLVPDVVAAAVAAGATIRDLHISEPSLENLFLHHTGRSLRE"
     gene            3233..>3699
                     /locus_tag="VNN25_15175"
     CDS             3233..>3699
                     /locus_tag="VNN25_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92924.1"
                     /translation="MSSTVNNKPATTTPDPAKLTPEAVIEQIRALRAQIDDVTPLSKA
                     QRNQLRGRTRKQPAPVVEASISVIGSSDTVAQAVGQPLVDVLQLQGDSVLWNLVADEL
                     RSFLKGIEGANLVRRQQLAFIASQAYSFGSQLARNPVNADLVPQVEEIKRLKVL"
BASE COUNT          705 a         1156 c         1166 g          672 t
ORIGIN      
        1 ccaacgacgc tacattgtgc ggcgtttcgg tgttcagttg attgtcgcgc tgctggggat
       61 cgcatgcgcg ttcctcgctc cggcactcgc actgagcatc ttcgtcgcgc tgccgattgt
      121 gttcgcgctg acctaccacc gcgggtacta ctgactgtcg cccggcacgg cgcaacgccg
      181 caatgtttcg cgcgcggcac atgatggtca cgtacgttcg cgggcacttc aagagcgtta
      241 gcagtggaat tgccgcaccg agctcccaat cgagcgcctt ctcccccacg cttctcagtg
      301 ggggagaagg tggccgaagg acggatgagg gggcgcttga ctatcgcgtc gacgttgcac
      361 aaacactgcg cggggatgtc ttttcaatca cgttaacagc tctcgattaa cgtgttggtc
      421 ccatcttgca acgcccccct catccggctt cgccaccttc tcccccgcaa gaagcgcggg
      481 gggagaaggc gctcgattgg aaggttgggg gtaagggttt gagaaatgcc ttcatcggca
      541 tatcaagtca gcgccttgcg ccggaactgg cgaaggccga aaaggaggag gcccgtgtcg
      601 aaggccacca gtgcggcgag aatgacggcc tgggggaggt gtggcgcctg tggcacgagg
      661 gcgctgcgaa agccctcgct ggcgtacacc agcggattga cgagcacgac gcgcttgagg
      721 atggggaact tggccagcgc gatccatggg taatacgtgc agccgaagaa gatcatcggg
      781 gccatgacca tgctgaacat cagaccgacc tgctgctgac cgatggagca gccgaggacc
      841 agaccgaggg tcgcggagag catcgccacc agcagcgcct ggaggatgaa gagtcccagc
      901 ttcgcgccgc tcatgttgac gccccccatc accagccatc cgaggggaat caccaccagg
      961 cctgacacga gagcctggat cattcccgcg acgaccttcc ccacagccag ccattcaaga
     1021 tccatggggg cgagcaggcg gtcctcgatc tccttggtga actggaactc agcgatgagc
     1081 ggcatggtca cggcccaggt gccggtcatg atcatgctca aggcgatgat gccgggaagg
     1141 agcagcgact tgtactccgc cgacatcagg ccgctggaag tcatcactct gccgaagacg
     1201 aatacgaaca tcatcggctg caacagagtc tgaaaggcca gttgcacgga gttgcgccgg
     1261 gccacgtgtg cgtcgcgccc cagcatggcc agaaaggtcg tcacgttcat tcccgaaggc
     1321 tccgtccggt gtggtgaaga aagaggttct cgagactggg ctcggaaatg tgcagatcgc
     1381 ggatggtggc acccgcggcg acggcggcgg cgacgacgtc ggggacgagc gagcctgcgt
     1441 ggtcggcata gacgtcgagc tgaccgtcac gaggctcgcg gacttcggac actccctgca
     1501 acagtcgcac ggcctcggcg aaaccggccg gcgttgtacc gacctgcagt cgcacgaggt
     1561 agccgccggg gatggactgc ttcaactctt taggcgttcc gacagcgatg accttgccgt
     1621 gatcgacgat ggccacgcgc tggcagaggc tgtcggcctc ctccatgttg tgcgtcgtca
     1681 gcaggatcgt cttcccgcgc gcgttgtacc ctcggatcat ctcactgagc aggaggcgcg
     1741 tctggggatc gagtcccgcg ctcggctcgt cgagaaacag gacgtcgggg tcgtgcatca
     1801 tggcccgggc gatggagagg cgttgcatca tcccgccgga aaacccgagc ggcttctgat
     1861 tggcccggtc ggtgagcttg aactgttcga gaagcgagtc ggcccgcgtc cgccgttcct
     1921 ccgaggagag gccgaagtag gcgccgtgaa aggtgaggat ctcccgcgcg gtgagggcga
     1981 agtcgagatt gggacgctgc ggcacgactc cgatcagccg cttgacggcc ttgctgttct
     2041 tccagacatc gaggtccccc acccaggcac ggccggccgt gggctggact cgggtggtga
     2101 cgatgccaac ggtggtggac ttgcccgcgc cgtttggccc gagcaacccg aagatctcgc
     2161 ccggccgcac ctccagcgtc agtccgtcga cggccacgac gtcgcctctc tgctgcgcgg
     2221 cagggtagac cttccgcaac ccttccgttc tgatcccgat gggcatgggg agtgattgta
     2281 aacgcagtgc cggtacgagg cggcgcgcgc gtcggcgaag caagcaacca cagagacaca
     2341 gagaacacgg agggtctctg tgacgcatca gcagttgggg agaaggcgct cgattggaaa
     2401 gagagagtta gaacaggcgc gcccgatact tccgcggacg tactccatag tccttacgcg
     2461 tcgactttcc gtctgctcgc cagcaaattc gcagcggctc ttgcgcgcga ggcgcagatc
     2521 ggcgtcgccc gctggctgcc atcctgatat cacacgcgac ctgcctgtgc ctcactggta
     2581 cagcgaccat gtaccactgg ggcaccggcc atgtcccact ggtacaccgc ccatctccca
     2641 ctggtacacc ggccatgtac cgctgggaca ccgaccgtgt accactggga cactggccat
     2701 gtaccactgg gacaccggcc atgtaccact ggtacaccga ccgtgtacca ctgggacatt
     2761 gaccgtgtac cgctgggaca tcgaccatgt accactgcga cactgactat gtaccactgg
     2821 gacaccgacc gtgtaccact ggggccaccc acgttgtgcc gcgttacatt ctcggcgcac
     2881 atgtgcgcgc gcatcgtgtt acacgatttt ttttgtatgt cccggcatcg ccccccttga
     2941 caagtcgttt caacgggctt atatcgttcg ctacaacgtc gaatttcgta agattttcgg
     3001 cgttggcggc tgtcggcggt acgggcgtgc tgaataagca catcagacct gtaccggcgc
     3061 gggagttgag ttgatctcct cctgccgcgg gcacccgagg tgtgtacaca ccgcgggaaa
     3121 acatcaaccg gaagaatcgg cgtgtggccg aacttccagc cggtggccgc tcgcacttgt
     3181 cgacgagtcg tgaacgtcac ctccggcctt gctcgcatta ggaggctttg tcatgagtag
     3241 taccgtcaat aacaaacctg ctacaacaac ccccgacccc gccaagctca cccccgaggc
     3301 ggtcatcgag cagatccgcg cattgcgtgc gcagatcgac gacgtgaccc cgctctcgaa
     3361 agcgcagcgg aaccagctca gagggcggac gcgcaagcag ccggccccgg tcgtcgaagc
     3421 gtcaatcagc gtgattggct cgtcggacac cgtggcgcag gctgtaggcc agccgctcgt
     3481 cgacgttctc cagcttcagg gcgactctgt cctctggaac ctcgtcgccg atgagctccg
     3541 ctcgttcctc aagggcatcg aaggggcgaa tctggtgcgc cgccagcagc tcgcgttcat
     3601 cgcatcgcag gcgtactcgt tcggatcgca gcttgcgcgg aacccggtca acgccgatct
     3661 ggtgccgcag gtggaggaga tcaagcgtct gaaggttct
//
LOCUS       DATLVW010000505         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_190205, whole genome shotgun sequence.
ACCESSION   DATLVW010000505 DATLVW010000000
VERSION     DATLVW010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190205"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VNN25_15180"
     CDS             <1..849
                     /locus_tag="VNN25_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH92925.1"
                     /translation="TYLMRGQAVEITVGPETNFLPTADLIRPHVRDARMICICTPANP
                     TGTVMARDEVERIARLVVEENERRAASGQRALLLLWDQIYWMLTFGGFQHYTPTKLVP
                     EATPYTIYVDGVSKAFAATGLRVGWTVAPPLITGRMRDILGHIGAWAPRPEQIAVARF
                     LDRRADIDEYHEGMIRELRLRLDLLYDGLEAMRREGIPVRAIAPQGAIYLSVQFDLIG
                     RGAITSNSQIRRLLLDDAGFAVVPFQAFGLPNESGWMRLSVGAVSPRQIEAGLARVRD
                     VLARYA"
     gene            874..>2421
                     /locus_tag="VNN25_15185"
     CDS             874..>2421
                     /locus_tag="VNN25_15185"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH92926.1"
                     /translation="MLSLRRLTVVLFLLAPLPALAQTTGSISGRVSASMSLLPGVTVE
                     GKSPALQGSRVAITNYEGIYHLSLLPPGTYTVTFSLSGFAPKSSTIVVPLGKEATLDA
                     DLSPSMSQTITVGESAPLVNETSTTLGTNLTASVIETLPTQRNYSSVVQVTPGVSTDA
                     IQQNDKQTASISVYGSSGAENAFYIDGVNTTGSEYGFQGKELNFEFIQALEVKTGGYE
                     AEYGRATGGIVNVITKSGSNQLAGDVFGYYDSDSLQTSSTRVVGGTPGGFKRKDYGAD
                     VGGALVKDKLWFFAALDQVHNTQDNVLSDGPLAGTSASSLSRRNLGSAKLTLSANPQH
                     TFTATWLQDPRADTGAINDANHTLIGDPSTYDGRQDFGGRDYALRYDGVVGSKWLFSA
                     QGARHRDTNSVGPATAAGDAIEYIDARNNFFQTGGFGLIQDKRFERKFYGGSATRFLG
                     AHQFKAGIEFQDETADVVKRMSGGQQVDIFENPNNPNKPIYSHFYWTTPDATVANAPV
                     SALNASPQ"
BASE COUNT          457 a          843 c          694 g          427 t
ORIGIN      
        1 acctacctga tgcgcggcca ggcggtagag atcactgtcg ggccggagac caacttcctc
       61 ccgaccgccg atctcatccg cccgcacgtt cgcgacgcgc gcatgatctg catctgcacg
      121 ccggccaatc cgaccggaac ggtcatggca cgcgacgaag tggaacggat cgcccgtctc
      181 gtcgttgagg aaaacgaacg ccgcgccgcc tcggggcagc gtgcactact ccttctctgg
      241 gaccagatct actggatgct cacgttcggc ggattccagc actacacgcc gactaagctc
      301 gtcccagagg ccacgccgta cacgatttat gtggacggcg tttccaaggc gttcgctgca
      361 accggattgc gcgtcggctg gaccgtcgcg ccgcccctga tcaccgggcg gatgcgcgac
      421 atcctcggcc acatcggcgc atgggctccc cgcccggagc agattgccgt cgccaggttc
      481 ctcgaccggc gtgccgacat cgacgagtat cacgaaggga tgatccgcga gctgcgcctg
      541 cgcctcgatc ttctttatga cggactcgag gccatgcggc gcgaagggat tccggtgcgc
      601 gccattgcgc cgcagggagc gatctacctc tccgtgcagt tcgatctgat cgggcgcggc
      661 gcaatcacgt cgaatagcca gatccgacga ctccttctcg acgacgccgg cttcgccgtc
      721 gtaccgttcc aggccttcgg gctgccgaac gagagcggct ggatgcggct cagcgtcggc
      781 gccgtttcgc cgcgccagat cgaagcgggg ttggcacgcg tgcgagacgt attggcgcgc
      841 tacgcgtaaa ctgtcctcca attaggaggt tccatgctct ctctccgccg tcttacggtc
      901 gtgctgtttc tgctcgcccc gctcccggcg ctcgcgcaga cgaccggctc catcagcggg
      961 cgtgtctctg ccagcatgtc gctgctcccc ggtgtcaccg tcgaagggaa aagccctgct
     1021 ctgcaaggct cccgcgtggc catcaccaac tacgagggta tctaccatct gtcgctgctc
     1081 ccacccggaa cctacacagt cactttctcg ctctcaggct tcgctccgaa atcgagcacc
     1141 attgtcgttc cccttgggaa agaggccaca cttgatgccg acctctcccc ttccatgagt
     1201 cagaccatca ccgtcggtga gtccgcgccg ctggtgaatg agacatcgac gaccctcggc
     1261 accaacctca ctgcctcggt catcgagact cttcccaccc agcgcaacta ctcctcggtc
     1321 gtgcaggtaa cgcccggtgt ttcgacggac gcaatccagc agaacgacaa gcagacggcg
     1381 tcgatctcgg tctacggttc gtccggcgcg gagaacgcct tctacatcga cggcgtgaac
     1441 accaccggct ccgagtacgg atttcagggg aaggagctca acttcgaatt catccaggcc
     1501 ctcgaagtga agacgggcgg atacgaggct gaatacggcc gcgccaccgg tggcatcgtc
     1561 aacgtgatca cgaagtcggg gagcaatcag ctcgccggcg acgtcttcgg ctattacgac
     1621 agcgactccc tgcagacgag ctcgacgaga gtcgtcggcg gcactccggg cggattcaag
     1681 aggaaggact acggcgccga tgtcgggggc gcactcgtca aagacaagct ctggttcttc
     1741 gccgcgctcg accaggtcca caacacacag gacaatgtgc tttcggacgg acctctcgcc
     1801 ggaacctctg cttcctcgct gagtcgccgc aacctcgggt cggccaagct gacgctcagc
     1861 gccaacccgc aacatacatt caccgccacg tggttgcagg accctcgtgc cgatacgggt
     1921 gcaatcaacg acgcgaatca cacgctcatc ggcgatccct cgacctacga cggccgccag
     1981 gacttcggcg gccgcgacta cgcgctccgt tacgacggcg tcgtcggatc gaagtggctc
     2041 ttctccgcgc agggcgcgcg ccatcgcgac accaactccg tcggaccggc tacggccgcc
     2101 ggcgacgcga tcgagtacat cgacgcccgc aacaacttct tccaaaccgg cggctttggt
     2161 ctgattcagg acaagagatt cgagcggaaa ttctacggcg gctccgctac tcgcttcctc
     2221 ggcgcgcacc agttcaaagc cggcatcgag tttcaggacg agaccgccga cgtcgtcaaa
     2281 cggatgtcag gcggccagca ggtcgacatc ttcgagaacc ccaacaatcc gaacaagccg
     2341 atctacagcc acttctactg gacgacgccc gacgccacag tcgccaatgc tcccgtctcg
     2401 gccctgaacg catcgccgca g
//
LOCUS       DATLVW010000506         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_600176, whole genome shotgun sequence.
ACCESSION   DATLVW010000506 DATLVW010000000
VERSION     DATLVW010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600176"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VNN25_15190"
     CDS             complement(<1..687)
                     /locus_tag="VNN25_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006669299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXH92927.1"
                     /translation="MAEAQFGVIGLAVMGENLAQNIEDHGFSVAVWNREPTVTDRFIA
                     KTAGKKFTGTKSLAELVKAIERPRRIVMMIKAGAPVDMTIDALMPLLDEGDVVIDGGN
                     SWFKDTQERTKRLEEKKLHFVGSGVSGGEEGARFGPSLMPGGSNESWEAIKPVFEAIA
                     AKSDSGPCVTHVGPDGAGHYVKMVHNGIEYGDMQLIAEAYEMLRKGLGANPEELAAIF
                     DEYSSKIAASS"
     gene            complement(700..3480)
                     /locus_tag="VNN25_15195"
     CDS             complement(700..3480)
                     /locus_tag="VNN25_15195"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HXH92928.1"
                     /translation="MSNPLRDLAKTGQSVWFDQMERRLVTSGELQRMIDEDDLRGLTS
                     NPTIFEKAIGGSDDYQAQLRTLASQNKSREEIYDAITIEDIGNAADVFRPVYDRCKGG
                     DGFCSLEVSPILARDTAGTAAEAKRLFSTLGRPNVMIKIPATPEGIPAIEESIAAGIN
                     INITLIFSNDVYAKVMEAYLRGLERRVAQNLPINEISSVASFFVSRIDALAEKQIEAK
                     LKEKDDPEVRGLMGKIAIANAKLAYQLFKETFSGARWEALRHHGANVQRPLWASTGTK
                     NPAYSDVLYIESLIGPDTVNTVPPATYKAFKDHGRVAMTLEADLDEARDVLRKFAEKG
                     FSLEAITDKLTDDGVKSFDESFASLLATIEAGRDEAIRGLARRQSLHLGAAQATVDQA
                     AQRIEKEKFVDRIWAKDATLWKNDDAHKKIIANALGWLVVAEEMAKAAPELMQFADSV
                     KADFDDVIVLGMGGSSLCSEVTRRLFGARSGYPRLNVLDSTVPQAIHALESNLNLART
                     LFIVASKSGTTTEPAMFHRYFYDRVKSVKGDKAGENFVAVTDPDTQLGRDAKADNFRK
                     IFINRADIGGRYSALSYFGMAPAAIAGVDVATLLGRAVHAGHIARAASAKKNPAAMLG
                     AVIGSLALQGRNKLTLITPAPLDTLGLWIEQLIAESTGKEGKGILPVAGEPLLNPADY
                     GNDRVFVCVRMRGSDMTSTLKALADAGHPVVDQVLDDPLDIGETFFTWEFATAVAGAL
                     IGIDAFDQPNVQESKDNTKRLLEEFKTSGTMHTDDHQVNPDDAAAIGALLASVKAGDY
                     VALTEYVVETPERDMAIAAIRETIGRELHVATTTGYGPRFLHSTGQLHKGGPDTGVYM
                     QLTGGGHEDVPIPNEKFSFGVLVRAQAIGDLQSLTSRKRRAISINLGENIDAGLATLT
                     TTVTNALSKVKA"
BASE COUNT          575 a         1112 c         1100 g          733 t
ORIGIN      
        1 ggagctggcg gcgatcttcg atgaatattc atcgaagatc gccgccagct cctcggggtt
       61 cgcccccagt cccttgcgca gcatctcgta cgcctcggcg atcagttgca tgtcgccgta
      121 ctcgatgccg ttgtgaacca tcttcacgta atgaccggcg ccgtcgggac cgacatgcgt
      181 cacgcaggga cccgaatcgc tcttcgccgc gatcgcttcg aagaccggct tgatcgcttc
      241 ccacgattcg ttcgagccgc cgggcatcag cgacggcccg aagcgcgcac cttcttcgcc
      301 gccagacacg cccgagccga cgaagtgcag cttcttctct tcgagccgct tcgttcgttc
      361 ctgcgtgtct ttgaaccagg agttgccgcc gtcgatgacg acgtcgcctt cgtcgagcag
      421 cggcatgagc gcgtcgatcg tcatgtcgac cggtgcgccg gccttgatca tcatcacgat
      481 ccggcgcggg cgctcgatcg ctttcaccag ctcggccaac gacttcgtac cggtgaactt
      541 ctttcccgcg gtcttggcga tgaagcggtc cgtcacggtc ggttcgcggt tccagaccgc
      601 aacggaaaag ccgtggtctt cgatgttttg cgcaaggttc tcgcccatca ccgcgagtcc
      661 gattacgccg aattgcgctt cagccattta cgtctccttt tatgctttga ccttcgacag
      721 ggcgttcgtc acggtggttg tcagcgtcgc caatcccgcg tcgatgttct cgccgaggtt
      781 gatgctgatc gcgcgccgct tccggctggt cagcgattgc agatcgccga tcgcctgcgc
      841 gcgcacgagc acgccgaaac tgaacttctc gttcggaatc ggcacgtcct cgtggccgcc
      901 gccagtgagt tgcatgtaga cgccggtatc cggcccccct ttgtgcaact gaccggtcga
      961 gtggaggaaa cgcggaccgt agccggtggt cgtggcgaca tgcagttcac ggccgatcgt
     1021 ttcccggatc gcggcgatcg ccatgtcgcg ctccggcgtc tccacgacat actccgtgag
     1081 tgccacgtaa tcgcccgcct tgacgctggc gagcagcgcc ccgatcgcgg cggcatcgtc
     1141 ggggttcacc tgatgatcat ccgtgtgcat cgtgccgctg gtcttgaact cctccagcag
     1201 gcgcttcgtg ttgtccttcg actcctgaac attcggctga tcgaaggcat caatgccgat
     1261 gagggcgccg gcgacagccg ttgcgaactc ccaggtaaag aacgtctcgc cgatgtcgag
     1321 cggatcatcg aggacctgat cgacgaccgg atggccggcg tcggcgagcg ctttgagcgt
     1381 tgacgtcata tccgagccgc gcatgcgcac gcagacgaag actcggtcgt tgccgtagtc
     1441 ggcaggattg agcagcggct cgccggcgac cggcaggatg cctttgccct ccttgcctgt
     1501 cgactccgcg atcagttgtt cgatccagag tccgagcgta tcgagtggcg cgggcgtgat
     1561 cagcgtgagc ttgttgcgcc cttggagcgc gagcgagccg atgaccgcgc cgagcatcgc
     1621 ggcaggattc ttcttcgcgc tggccgcgcg ggcgatgtgg ccggcgtgca ccgcgcggcc
     1681 gaggagcgtg gccacatcga cgccggcaat tgcggcaggc gccattccga aataggaaag
     1741 ggctgaatag cggccgccga tgtccgcgcg attgatgaag atcttgcgga agttgtccgc
     1801 cttcgcgtcg cgtccgagct gcgtgtcggg atcggtcacg gcaacgaaat tctcgcccgc
     1861 cttgtcgccc ttgaccgact tcacgcgatc gtagaagtag cgatgaaaca tggccggctc
     1921 ggtcgtcgtg cccgatttgg aggcgacgat gaacagcgtg cgcgccaggt tgaggttcga
     1981 ctccagcgcg tgtatcgcct gaggcacggt cgagtcgagc acgttcagcc gcggatagcc
     2041 gctccgcgct ccaaacaacc gccgcgtcac ctcggaacag aggctcgaac cacccatgcc
     2101 gaggacgatg acgtcatcga aatcggcctt cacggaatcg gcgaactgca tgagctccgg
     2161 tgccgccttc gccatctcct cggccacaac gagccagccg agggcgttgg cgatgatctt
     2221 cttgtgcgcg tcgtcgttct tccagagcgt ggcgtccttg gcccaaatgc ggtcgacgaa
     2281 cttctctttc tcgatgcgct gcgcagcctg atcgacggtg gcctgagcgg cgccgaggtg
     2341 cagcgattgg cggcgggcca gtccgcggat ggcttcatcg cgccctgcct caatcgtcgc
     2401 caggagcgac gcgaacgact catcgaatga tttcacgcca tcgtccgtga gcttgtcggt
     2461 gatcgcttcg aggctgaatc ctttttcagc gaacttgcgc agcacgtcgc gcgcttcgtc
     2521 cagatccgct tccagcgtca tggccacgcg gccgtgatct ttgaacgcct tgtacgtcgc
     2581 gggcggaacg gtgttaacgg tgtcgggacc gatgagcgac tcgatgtaga gcacgtcgct
     2641 gtaggccgga ttcttggtgc cggtcgatgc ccacaacgga cgctgcacgt tggcgccgtg
     2701 atggcgcagc gcttcccatc gcgcaccgct gaaagtctcc ttgaagagct gataggcgag
     2761 cttggcgttg gcgatggcga tctttcccat caacccccga acctcgggat cgtccttttc
     2821 cttgagcttc gcctcgatct gcttctcggc cagcgcgtcg atgcggctga cgaagaaact
     2881 ggcgactgag gagatctcgt tgatcggcag gttctgcgcg acgcgccgct cgagaccgcg
     2941 gaggtacgcc tccatcacct tcgcgtagac gtcgttggag aagattagcg tgatgttgat
     3001 gttgatcccg gctgcgatcg actcctcgat ggccgggatt ccttccggcg tcgccgggat
     3061 cttgatcatc acgttcggcc ggccgagcgt ggagaagaga cgtttggctt cggcagcggt
     3121 gccggcggtg tcgcgcgcaa gaatcggcga gacctccaag ctgcagaacc catcgccacc
     3181 tttgcagcgg tcgtagacgg ggcggaaaac gtcggcggcg ttgccgatgt cttcgatcgt
     3241 gatggcgtcg tagatctctt cgcgcgattt gttctgcgac gccagggtgc gcaactgcgc
     3301 ctggtagtcg tcgctgccgc cgatcgcttt ctcgaagatg gtcgggttcg aagtgaggcc
     3361 gcgcagatcg tcttcatcga tcatccgctg caattcgccg ctggtgacga ggcgccgctc
     3421 catctgatca aaccagacgc tctggccggt cttcgccaga tctctcaatg gattgctcat
     3481 cttgacctcc gcagtcgaaa gcatatcgcg tgcgcggggg
//
LOCUS       DATLVW010000507         5710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_557619, whole genome shotgun sequence.
ACCESSION   DATLVW010000507 DATLVW010000000
VERSION     DATLVW010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557619"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNN25_15200"
     CDS             complement(<1..183)
                     /locus_tag="VNN25_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92929.1"
                     /translation="MDVVQPTAAADHHDGASMRRSLAERGVIVMKESRVIARIAPTIG
                     GSLDIAGLIIAIIKNGV"
     gene            307..383
                     /locus_tag="VNN25_15205"
     tRNA            307..383
                     /locus_tag="VNN25_15205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Arg,seq:cct)
     gene            611..1231
                     /locus_tag="VNN25_15210"
     CDS             611..1231
                     /locus_tag="VNN25_15210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXH92930.1"
                     /translation="MLSLILGLSLSAQTNPTEQPRKCHWWQFGRCDEVEGIEGLPPDA
                     PRTGTVITVDVSTNILYLFKNGQLIERSPAATGTEKLLKHAGHVWLFRTPQGHMKVLR
                     KIVDPVWRKPDWAFVEAGERVPPRDSPRRNVHGHLGKYALDLGDGILIHGTDEARSIG
                     RRASHGCIRLPDDMLKFLFKSVPVGTDVFIFESQMRQTVERHSDLD"
     gene            complement(1270..1698)
                     /locus_tag="VNN25_15215"
     CDS             complement(1270..1698)
                     /locus_tag="VNN25_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92931.1"
                     /translation="MIFTRKTRFSADKRDFRPKNATTRRMNAFFARKTRFSPEKRDHT
                     LGERDFRRKNATARRMNTFFARKTRFAADQRDSTPAARDCGRGHPSRRRAACGTAAEP
                     AALLLYLKPRGSLDKRRQEERSASGPLAGPPSARRRLSIA"
     gene            complement(1863..3215)
                     /locus_tag="VNN25_15220"
     CDS             complement(1863..3215)
                     /locus_tag="VNN25_15220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH92932.1"
                     /translation="MSDSEIRQRLLIVDDDPNIVAGLAALLSDEWDVKTALLARDART
                     VFADFSPDVVLLDMKLPDGDGVELLHDLKMYSEAVSVIMMSGEGTIDRVVESMKLGAE
                     TFLQKPFDNSTLTLTLQQVSRIAATRREVIALRRGDSNDLERLPGISPAVQNLNQLLG
                     QIARAPSPVLIEGDSGTGKGVFARLIHNRSPRARAPFVDLNCAGLSKELLESELFGHE
                     RGAFTNAMNTKPGLFEIASDGTLFLDEIGDMDVSVQARLLKAIEEKRFRRVGGIRDLT
                     ANFRLLAATNRDLGKDVGTGRFRGDLFYRLNVVRVRMPPLRERLEDIPLLVDVILRPL
                     AKEMGRPAPPVSPRAIKKLESYAWPGNVRELRNVLERAMLTMTAKEIRSEDLLLESDQ
                     AAIATKSGGLPTADWEIRPLEDVVADYVTASVDAAGGNVRKAARMLKISPSTLYARKK
                     "
     gene            3315..3686
                     /locus_tag="VNN25_15225"
     CDS             3315..3686
                     /locus_tag="VNN25_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92933.1"
                     /translation="MVSTLEYVAAMNILHAITLAAFLHHSPAPIPVCHSGTVSYKFVG
                     TPGATFVYAGDRYSIHASGWVELLSSEKIYLAANGRTLPLDVWPIDAFGTRTVPLQAV
                     PETSPNSPANSVTTVASNTIN"
     gene            3715..3849
                     /locus_tag="VNN25_15230"
     CDS             3715..3849
                     /locus_tag="VNN25_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92934.1"
                     /translation="MAQIKHFVIETTSDLRSGRNSWRVMSALWSAALLISVTTAVFHH
                     "
     gene            3863..4234
                     /locus_tag="VNN25_15235"
     CDS             3863..4234
                     /locus_tag="VNN25_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92935.1"
                     /translation="MFNDPRRITLLARASSSPARAWNVSGKAPNRVIFVDAVSMLNFA
                     LDRANHDVDRVLIDGTATAAEYLELLSGLPLGFVGDVLYMRAEGNSFLSTVGRGGGRL
                     LYSLTPNDVEFYLQTFRLLAA"
     gene            4285..4938
                     /locus_tag="VNN25_15240"
     CDS             4285..4938
                     /locus_tag="VNN25_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXH92936.1"
                     /translation="MQLWTSFLHTGWVGKAVLGVLLFFSVGSWSVIFVIALRFRRSAA
                     ASRRFMFAFRRAKKLADVQAVTAQETHSSLVGLFKAGYAEIEAQIAHANAATIRSLDS
                     VERSLLRAARIEAARMSRYLPFLATTAAASPFIGLFGTVWGIMDAFATIGAMQTSSIT
                     AVAPGISEALINTAAGLFAAIPALLAYNHFVQRMRNARGEMEDFTLEFLNLTERNFM"
     gene            5050..5487
                     /locus_tag="VNN25_15245"
     CDS             5050..5487
                     /locus_tag="VNN25_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HXH92937.1"
                     /translation="MSFRFDHGDDYGDMAEINVTPLVDVMLVLLIIFMVTAPMLTQGL
                     TVALPAAEGKSFELASNNPAKIQVTAAGAVYLDGIAAGSTNLDLTLGPMLRARRVKRA
                     LLEADKAVPYGRVVAVLDIMNRAGVEQLGMVTQPKEANARPRR"
     gene            5468..>5710
                     /locus_tag="VNN25_15250"
     CDS             5468..>5710
                     /locus_tag="VNN25_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92938.1"
                     /translation="MRDRVGDLLAERARLSGGAGGGIALSILLHGSIVAVIVYTAMHA
                     RAPQQVSTLSIQFAPTQAIQQPATPAPTPHAPQSAPP"
BASE COUNT         1033 a         1837 c         1734 g         1106 t
ORIGIN      
        1 cacgccgttc ttgatgatgg cgatgatgag gccggcgata tcgagagagc cgccgatcgt
       61 tggagcgatc cgcgcgatca cacggctctc cttcatgacg atgacaccgc gctcggccag
      121 cgaacgtctc atcgacgcgc cgtcatggtg gtcggcagct gcggtcggtt gaacaacatc
      181 catcgcagca tggtacgcgg aacttgaacg acggtgaggt tctcccactc cccacaccca
      241 actccccaca cccaactccc cactcccgga ctttctgcta ccctcccgcc gtttcccgcc
      301 gcgagtggcc ccgtagctca ggtggataga gcagcggttt cctaaaccgt ttgtcgggcg
      361 ttcgagtcgc cccggggcca ccagaatcct tcctaccggg ggtcgtgttt cgggggtcgg
      421 gagtcggagc aaggcatcgc gatgtttcga tgcgtgctcc gactcccgac ccccgacttc
      481 caccgatcaa cggccccatc ccacccccca cacccaactc cccacacccc gcttctggcg
      541 tcgatctcgc accgagttat tcgcagtgcg atggaatccg tcaacgaaat tgctcagccg
      601 aggactcctc gtgttgagtt tgatcctcgg actctcgctg tctgcccaaa cgaatccaac
      661 ggagcagccg aggaagtgtc actggtggca gtttggccgt tgcgatgaag tggaggggat
      721 cgaaggactg ccccccgatg ctccgcggac cgggacggtg atcacggtcg acgtctcgac
      781 caacatcctc tacctcttca agaacggcca gctcatagag agatcgcccg ccgcgaccgg
      841 aaccgagaag ctcttgaagc acgcgggcca cgtgtggctg tttcgcacgc cgcagggaca
      901 catgaaagtg ctgcgcaaga tcgtcgaccc tgtctggaga aagccggact gggccttcgt
      961 ggaagcggga gagcgggttc caccgcgcga ttcaccgcgt cgcaatgtcc acgggcacct
     1021 cggcaagtac gcgctcgacc tcggcgacgg catcctgatc cacggcaccg acgaagcccg
     1081 ctcgatcggc cgccgagcct cgcatggctg catccgtctt ccagacgaca tgctcaagtt
     1141 cctgttcaag agcgtccccg tgggtaccga cgtcttcatc ttcgagagtc agatgcgcca
     1201 gacggtggaa cggcacagtg atttggattg aggggagtgg ggtgtgggga gtggggaacg
     1261 atgcttttgt caggcgattg agagtcgccg tcgagccgac ggcggtccag ccagcgggcc
     1321 gctggcgcta cgttcttcct gtcgacgttt atcgagagaa cctcggggtt taaggtagag
     1381 gagtagcgcc gccggctcgg cggctgtacc gcaggcggct cgcctgcgac tggggtgtcc
     1441 tctcccgcag tcgcgtgcag ccggtgtgct gtcgcgttga tcggccgcaa aacgcgtttt
     1501 tcgggcgaaa aacgtgttca tccggcgtgc tgtcgcgttt tttcgtcgaa aatcgcgttc
     1561 acccagcgtg tgatcgcgtt tttcaggcga aaaacgcgtt tttcgggcga aaaacgcgtt
     1621 catccggcgt gttgtcgcgt ttttcgggcg aaaatcgcgt ttatccgccg aaaatcgtgt
     1681 ttttcgggtg aaaatcatgt ttttcgggtg aaaatcgcgt ttttcgcgtg aaaatcgcgt
     1741 tcatccgagg gattgtcgcg gtcctccgtc cggctgcgcc gcacccggac ggtttgtcag
     1801 ctcgttgcct gacggaacat gtctcccacc ccccacatcc cactccccac ccccccgaaa
     1861 ccctacttct tcctcgcata cagcgtcgac gggctgatct tcagcatgcg cgcggctttg
     1921 cgcacgtttc cgccggcggc gtcgaccgag gcggtgacgt agtcggcgac gacgtcttcg
     1981 agcggacgga tctcccaatc ggcagttggc agaccgccgg acttggtcgc gatcgcggcc
     2041 tggtccgatt ccaggagcag atcctcgctg cggatctcct ttgcggtcat cgtcagcatg
     2101 gcgcgctcga ggacgttgcg cagctcgcgc acgttgcccg gccaggcgta gctctctaat
     2161 ttttttatcg cccgggggct gactggtggt gcggggcggc ccatctcttt cgccagcgga
     2221 cgcaagatca cgtcgacgag cagcgggatg tcttcgaggc gctcgcgaag agggggcatg
     2281 cggacgcgga cgacattgag gcggtagaag agatcgccgc ggaaacggcc ggtgccgacg
     2341 tctttgccga ggtcgcggtt ggtggcggcg aggagccgga agttagctgt cagatcgcgg
     2401 atcccgccga cgcgtcgaaa gcgtttttcc tcgatcgcct tcagaagtcg ggcctggacc
     2461 gaaacgtcca tgtcgccgat ctcatcaagg aacagcgttc cgtcggacgc gatctcgaaa
     2521 aggcccggct tcgtgttcat cgcgttcgtg aaggcgccgc gctcgtggcc gaacagctcg
     2581 gattcgagca gctccttcga gagaccggcg caattcagat cgacgaacgg cgcgcgggca
     2641 cgcggcgagc ggttgtgaat gagccgcgcg aagacgccct tgcccgttcc ggagtcgccc
     2701 tcgatgagaa ccggcgatgg cgcacgcgcg atctggccga ggagctggtt cagattctgg
     2761 acggcggggg agatgcccgg caggcgctcc aggtcattcg agtcgccccg gcggagggcg
     2821 atgacttcgc ggcgcgtggc ggcgatgcgg gagacctgct ggagcgtcag cgtcagcgtg
     2881 ctgttatcga acggtttctg aagaaacgtc tcggcgccga gcttcatcga ctcgacgacg
     2941 cggtcgatgg tgccctcgcc cgacatcatg atcaccgaca ccgcctcgga gtacattttc
     3001 aggtcgtgca gcagttccac gccgtccccg tccgggagct tcatgtccag aagaacgaca
     3061 tcgggcgaga agtcggcaaa gaccgtccgc gcatcgcggg ccaggagcgc ggtcttgacg
     3121 tcccattcgt cggagagaag ggcagccagg ccggcgacga tgttcgggtc gtcgtcgacg
     3181 atcagcaggc gttgtctgat ttctgaatcg ctcaatgtcc gaaaatcata cgtctgtatg
     3241 gttcctaaac agcgttcaat ccgccgcgat cagctaactc tctgatttca aagcgatttc
     3301 aaagcaatgg cctgatggta tcgacgttgg agtatgtcgc tgccatgaac atcctgcacg
     3361 caatcaccct ggccgccttc cttcaccact cgccagcccc gatccctgtt tgccactcgg
     3421 gaacggtgtc ctacaagttc gtcggtacgc cgggcgcgac cttcgtctac gcaggcgacc
     3481 gctactcgat tcatgccagc ggatgggtgg aactgttgag cagcgagaag atctacctcg
     3541 ctgcgaatgg caggacatta ccgctcgacg tgtggcccat cgacgcgttc ggaacacgga
     3601 cggttcctct gcaggcggtt ccggagacga gccccaactc accagcgaat tcggtcacga
     3661 cagtggccag caacaccatc aactaacggc gtcaacacca caaggagaac tcacatggct
     3721 caaatcaaac acttcgtcat cgagaccacc tcggatcttc gcagcggacg taactcctgg
     3781 cgtgtcatga gtgcgctctg gtccgccgca ctcctcatct cagtcaccac cgcggtgttt
     3841 catcactgaa gggaggtccg cgatgttcaa cgatccccgc cgcatcaccc ttctcgcccg
     3901 tgcctcctcc tcgcctgcgc gagcatggaa cgtctccgga aaggcgccga accgcgtgat
     3961 cttcgtcgac gcggtttcga tgctcaactt cgccctcgac cgcgccaacc acgacgtcga
     4021 ccgcgtgctg atcgacggca cagcgacggc ggccgagtat ctggaattgc tgtccgggtt
     4081 gcccctcggg ttcgtgggcg acgtgctcta catgcgagca gagggaaact cgttcctcag
     4141 caccgtcgga cgcggcggcg gccggctgct ctactcgctg acgccgaatg acgtcgagtt
     4201 ctatctgcaa acctttcgat tacttgccgc ctgagtccgc aacgaagggg cgcacggccg
     4261 cagcgacgtg ctacctttat cgcagtgcaa ctctggacgt cgtttctgca tacggggtgg
     4321 gtggggaagg ccgtcctcgg tgtactcctc ttcttctccg tcggatcgtg gtcggtgatc
     4381 ttcgtgatcg cgctacgctt tcggcgatcc gccgccgcat cgcgccgttt catgttcgcg
     4441 ttccgccgcg ccaagaagct ggccgacgtg caggcagtga cggcgcagga gacacactct
     4501 tcgctggtcg gcctgttcaa agcaggctac gcggagatcg aagcgcagat cgcgcacgcg
     4561 aacgcggcca ccattcgatc gctcgatagc gtggagcggt cactgctgcg cgcggcgcgg
     4621 atcgaagcgg cccggatgtc ccgctatctc ccgttcctcg ccacgaccgc cgctgcgtcg
     4681 ccgttcatcg gactcttcgg cacggtgtgg ggaatcatgg atgccttcgc cacgatcgga
     4741 gcgatgcaga cctcgtcgat caccgcggtc gctcccggca tcagcgaagc gctcatcaac
     4801 accgccgcag gcctcttcgc agcaatcccc gcgctgctcg cctacaacca cttcgttcaa
     4861 cgcatgcgca acgcgcgcgg cgagatggag gactttacgc tggagttctt gaatcttacg
     4921 gagagaaatt ttatgtagtg ggcaacgtag gtcggactct ctagtccgac tgcttccgat
     4981 gtcggactag agagtccgac ctacgttgcg gttacttaac ctttcgcacg ggcgtctaac
     5041 caacacacaa tgtcctttcg attcgatcac ggcgatgact acggcgacat ggcggagatc
     5101 aatgtcacgc cgctcgtcga cgtcatgttg gtgctgctga tcatcttcat ggtcaccgcg
     5161 ccgatgctga cgcaagggtt gaccgtggcg ctccccgcag cggagggaaa gagcttcgag
     5221 ctggcgtcga acaacccggc aaagattcag gtgaccgcgg caggagcggt ctatctcgac
     5281 ggcatcgcgg ccggctcgac caatctcgat ctcacgctcg ggccgatgct gcgcgcgcgg
     5341 cgcgtcaaac gcgcgctgct cgaagccgac aaagccgtgc cgtacggccg cgtcgtcgcg
     5401 gtgctcgaca tcatgaaccg cgccggcgtc gagcagctcg ggatggtcac ccaaccgaag
     5461 gaggcgaatg cgcgaccgcg tcggtgacct gctcgccgag cgcgcccgcc tgagcggcgg
     5521 cgctggtggc ggcatcgccc tctcgatcct gctgcatgga tcgatcgttg cagtaatcgt
     5581 ctacaccgcg atgcacgcaa gggcgccgca gcaggtctcg acgctcagca tccaattcgc
     5641 gccgacgcaa gcaatccaac aaccggcgac gccagctccc acaccccacg ccccacaatc
     5701 cgcacccccc
//
LOCUS       DATLVW010000508         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_271128, whole genome shotgun sequence.
ACCESSION   DATLVW010000508 DATLVW010000000
VERSION     DATLVW010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271128"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VNN25_15255"
     CDS             complement(<1..558)
                     /locus_tag="VNN25_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92939.1"
                     /translation="MDLQNELFVSRKKAKRGKATIASLIVHGGIIALIVFISTQAAQR
                     VAAENKPIHAFLSSSAPPPPPPPPPPPAASHAASTPKPVVKPVQIPQQTFVQPREIPK
                     EVPKVDIPTPTTTAPVDTTPAQPANDPPGAQEGGVPGGVVGGVVGGQTGGTVGGEVGG
                     QIGGVLGGEKGGTPGGQVGGTGEGTG"
     gene            677..1723
                     /locus_tag="VNN25_15260"
     CDS             677..1723
                     /locus_tag="VNN25_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92940.1"
                     /translation="MKLGLSMGLLLLSGCASSSIPIPAAWQTVPQAALVGDYCAHVSC
                     AAKPQTAIHVVGNRIFNGTKGLTPPFAAIDSFDVSLDRKEVVFSARRKDNFDIGLVSM
                     DGSDIHWVPEDPADETDVQWAPRGNKVSFVLHTATGSIVRTVHIPTATPLSTDFPQTQ
                     IDALAWEPEAERYAVVVESPQSSQSLQEMTYAGEKRREVIPPAARLDVSIEPIGGVLV
                     MRPNDLHYGEKVPIVLWLDPHPFAWSDARAALMRNARVAIAVAPRENETFWGEIAKTP
                     WIDATRRYVVTSTPEHPGNPSATLITADPAIPAGCYRQTGNTVSAPPAVVQSFAAAWI
                     ADDLKGTPPPNGRR"
     gene            1710..2468
                     /gene="lepB"
                     /locus_tag="VNN25_15265"
     CDS             1710..2468
                     /gene="lepB"
                     /locus_tag="VNN25_15265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXH92941.1"
                     /translation="MAVDETNKKSTIREYYEALLIAVIFVNFARIFAFQAFKIPTGSM
                     EDNLKVGDHIIVNKFIYGPGTTLGGMLPLREIRRGDIIVFRYPLQPDTDFVKRVIGLP
                     GDTVEVHDKVVSVNGKPLAEPYVIHVDPTIYPPRPQLPEPYRSRDQFGPYFVPVGQYF
                     AMGDNRDRSSDSRYWGTVPRSMIKGKAFMVYWSFEGTPPSPDAPSSARIKELAGVVVH
                     FFTKTRWERTFFVVDSKYHFTPGLTPEGEAGESP"
     gene            2465..2737
                     /locus_tag="VNN25_15270"
     CDS             2465..2737
                     /locus_tag="VNN25_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92942.1"
                     /translation="MSDSREWGVGGGEWENGSPAEPTMPVRESELAREPVVGSRESEL
                     SEAGEPSTLTSIQTMTLDDRAESPDVLDLATTPNTPLPPPTTPRNP"
BASE COUNT          541 a          954 c          847 g          523 t
ORIGIN      
        1 acccgttcct tcgcctgtgc cgccgacctg cccgcccggc gtgccgccct tttcaccacc
       61 gaggacgccg ccgatctggc caccgacctc gccgccgaca gttccgcccg tctgtccacc
      121 gactacgcct ccaacgacgc cgccgggaac gccaccttcc tgtgcgcccg gcggatcatt
      181 cgccggctgg gccggtgtgg tatcgactgg cgcggttgtg gtcggtgtgg ggatatcaac
      241 cttcggcact tctttcggaa tctcgcgcgg ttgcacgaag gtctgctgag ggatctgaac
      301 cggcttgacg accggcttcg gtgtcgaggc cgcatgcgag gccgccgggg gcggcggcgg
      361 aggcggcggt gggggagggg ccgatgacga taagaacgcg tgaatcggct tgttttcggc
      421 ggcgactctc tgcgccgcct gcgtggagat gaaaacgatc agggcgatga ttccgccgtg
      481 aacgataagg gaggcgatcg tcgccttccc tcgctttgct ttcttccggg atacaaacag
      541 ttcgttttgg agatccatat cgttgtgtct cctcactcga tttcgtttag ttattgagca
      601 atattgcagc caagtgttca catcgatttg cactagtaaa atcaatcatt taggccgcgt
      661 gctagaatcc gccagcatga aactaggtct atcgatgggg ctgcttctgc tcagcgggtg
      721 cgcaagctcg tcgataccga ttcccgccgc ttggcagacc gtgccgcaag ccgcgctggt
      781 cggcgactat tgcgcgcatg tctcgtgtgc ggcgaagccc cagaccgcga ttcacgtggt
      841 gggaaaccga atcttcaacg gcaccaaagg tctgacccca ccctttgctg cgatcgactc
      901 cttcgatgtt tcgctcgatc ggaaggaagt cgtcttctcc gcgcgccgga aggacaactt
      961 cgacatcgga ctcgtttcga tggacggcag cgacatccac tgggttcccg aagatccggc
     1021 tgatgagacg gacgtccagt gggcgccgcg aggaaacaag gtcagcttcg ttctgcacac
     1081 ggcgacaggc agcatcgttc gcaccgtgca catcccgacc gcgacgccgc tgtcgaccga
     1141 ctttccgcaa acgcagatcg atgccctggc ctgggaaccg gaggcggagc gctatgcggt
     1201 cgtcgtcgag tcgccgcaat cgtcgcaaag tcttcaggag atgacctatg caggcgagaa
     1261 gcggagggaa gtgattccgc ccgcggcgcg tctcgacgtc tcgatcgagc cgatcggcgg
     1321 agtgctcgtg atgcgcccca acgacctgca ctacggcgag aaggttccga tcgtcctctg
     1381 gctcgaccct catccgttcg cctggagcga cgcccgcgcc gcgctcatgc gcaacgcacg
     1441 agtggcgatc gccgtcgcac cgcgagagaa cgaaacgttc tggggcgaga tcgcgaagac
     1501 cccatggatc gacgccacgc gaaggtacgt ggtcacttcc acccccgagc accccggcaa
     1561 cccttcagct accctgatca cagcagaccc ggccattcct gccggctgct accggcagac
     1621 cggaaacacc gtctccgccc cacccgctgt cgtacaatca tttgccgccg catggattgc
     1681 ggacgacctg aagggaacac ccccgccgaa tggccgtcga tgaaaccaac aagaaatcga
     1741 cgatccgcga gtactacgaa gcactcctga tcgccgtcat cttcgtcaac tttgcccgta
     1801 tcttcgcctt ccaggccttc aagatcccca ccggatcgat ggaggacaac ctgaaagtcg
     1861 gcgatcacat catcgtcaac aagttcattt acggacctgg gacgacccta ggcgggatgc
     1921 tgccgctacg cgagatccgc cgcggcgaca tcatcgtctt ccgctacccg ctgcagcccg
     1981 acaccgactt cgtgaagcgc gtcatcggcc tgcccggcga taccgtggaa gttcacgaca
     2041 aggtcgtgtc ggtcaacgga aagccactgg cggagccgta cgtgatccac gtcgatccga
     2101 cgatctatcc tccccgtccg caacttccgg agccgtatcg atcgcgcgat cagttcgggc
     2161 cgtactttgt cccggttggt cagtacttcg cgatgggaga caaccgcgat cgctccagcg
     2221 actcgcggta ttggggaacc gtgccccgct cgatgatcaa gggcaaggcg ttcatggtct
     2281 attggtcatt cgaaggaacg cctccgtcgc ccgacgcacc gtcgtccgcg aggatcaagg
     2341 aactggccgg cgtcgtggtc cacttcttca cgaaaacgcg ctgggaacgg acgttcttcg
     2401 tcgtcgacag caagtaccat ttcacgccgg gtttgacgcc cgagggcgag gccggcgaaa
     2461 gcccatgagt gattcgaggg agtggggagt ggggggtggg gagtgggaga acggctcgcc
     2521 agccgagccc acgatgcccg tgcgggagtc ggagctggcg cgggagccgg ttgtcgggag
     2581 tcgggagtcg gagctctccg aagccggcga gccttcgaca ttaacatcga tccagacgat
     2641 gacgctcgac gaccgagcgg aatctcctga cgttcttgac ttggctacca ctcccaacac
     2701 cccactccca ccccccacga ccccgaggaa tccttagcgc cagctccgac ccccgacccc
     2761 cgactggcga ctcccgaaga agctcccacc ccccacgccc cacccctcac ccccgaacca
     2821 gaccccatct acgtcgaccg cagcgaccat cccatctcgc ggcgg
//
LOCUS       DATLVW010000509         6367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_432812, whole genome shotgun sequence.
ACCESSION   DATLVW010000509 DATLVW010000000
VERSION     DATLVW010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432812"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VNN25_15275"
     CDS             <1..1068
                     /locus_tag="VNN25_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXH92943.1"
                     /translation="DVTYYRERLREKRYAVDQEALREYFPTMPCVKWMLDITERLYGI
                     QFREAIVPVWHDDVIYYDVDDVVTGERIGGIYLDLYPRDDKYKHAAAWPVRGVSRRTA
                     RKPISVLVTNFNRQGLTHSEVETLLHEFGHVMHGVLSRTEYNQHSGTSVERDFVEAPS
                     QMYEEWASRMESLALMRNHCTTCPLVDESLVQRIRAAKKFGSGIDYGRQLLYASYDMA
                     LAGVGNRGSGVREQAAQASAPTPDSRNSTPDSPSLELWRNMEGSTPMGTIDGTAFPGT
                     FDHIASGYAAGYYGYMWAKVIALDLISAFGGDLMNAATGRRFRELILARGSEEPARDL
                     VEQFLGRPVSSEAFFAEIRGE"
     gene            1236..1514
                     /locus_tag="VNN25_15280"
     CDS             1236..1514
                     /locus_tag="VNN25_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXH92944.1"
                     /translation="MKVYFGNLPKDIDDAKLAELVKPFGTPATAEVVNDRVTGLSKGF
                     AFVEFANDAEAQATIKGLDGTDVNGQAVVVNEARSKKDAPKPAPAVQH"
     gene            1530..1748
                     /locus_tag="VNN25_15285"
     CDS             1530..1748
                     /locus_tag="VNN25_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92945.1"
                     /translation="MTNVLKPKRAAKVTVSESNIRKAAARLSQRPLVSPEVQYIQRVL
                     GATATQDAVDEKVIAVRKLPWASIVIPE"
     gene            complement(1836..3248)
                     /locus_tag="VNN25_15290"
     CDS             complement(1836..3248)
                     /locus_tag="VNN25_15290"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HXH92946.1"
                     /translation="MRSPLLLILSLACWPALAQTVPQAFGPPAAAPPAELSTIVVPIK
                     ASLAPLLPQLESQVPKSLSKTDAFELDPLKQFGMKYRVVRDPIVLNAIGGGIHATTTM
                     HFALEGCRRTKKPFSDEIVMWPCLSCGFGEPMRDAWISLSSHLEWDANWRLRSTTRAM
                     PVDFSSNRCQVTFANIDITDWKIAPIVKDQLRQVLKTIDANTPKLTNIRPAASQVWSS
                     LQTPVEIASRMWLVMEPAEVSLGPITGTGLSVTSTLTLRARTRVIVGEKPPVVQKPLP
                     PLRVSPASTGGIRIPFDVELSYDEASRVMTENFGKRKYEGVAVDSIRIAPAADGRVSI
                     ELIVDYRESRLRHYNGPVNLDAAPVFDAATRTISLQNLEYTLDSKRKNLFVRIADRLA
                     HESLRAQLAKTAHWSVAPQIDSIRSAIASAVTRPLAPGVVMRGYVDGVELRSMTLDAN
                     GIVIHALATGNAEVDISAFR"
     gene            3438..3821
                     /gene="apaG"
                     /locus_tag="VNN25_15295"
     CDS             3438..3821
                     /gene="apaG"
                     /locus_tag="VNN25_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HXH92947.1"
                     /translation="MSDTTTRGIRIQVESIYVEERSDPQESYFFFAYHVVISNVGSET
                     AQLMSREWIITDSDGNVERVEGDGVVGEQPLLAPGQVFEYTSFCPLRTSMGSMQGSYL
                     MRPPGGESFRAEIAAFTLAVPGVVN"
     gene            3831..5405
                     /locus_tag="VNN25_15300"
     CDS             3831..5405
                     /locus_tag="VNN25_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92948.1"
                     /translation="MRRRVQISFLSAFLTLLTVAFAAALVVRVRSYSGPMTTESSASA
                     ATAAGTRAAARSNRRTDGLSGDDPLPTGSTVSTQGGSTASNATAAAARAKRFQELLAL
                     GAPKLDAPVSPVQAPVKIVPVPVVAKVQTPVSRPPQAAANAFRGSGASGSAGSNGPPS
                     SNNKPNTPTNDPKDPNSDTVPPQLISIAFDPPQVSDGGDTTLVVNATDDLSGIRGISG
                     TLTSPNGKAVQGFAQQREGETTRYVSRIHIPEKAEEGFWKITFLTMSDMATNSVTLSA
                     GQGTLPPTAVLKVTSSASDSTAPTLRNVWLARGAMRAGEHNTLYVDAQDDKSGVHLVA
                     AVFLSPSKLARFGVGCHTGADNVWECDVFPPTCLDCGDWTLEQVQLQDNANNFATIRS
                     ENPAVQSVRLNITGDTCDNQPPVLQGLALSRTEIVMGRDEPVIEFVAQVSDDSCGIGG
                     VSGQIVGPGGNSSGTFFSLQQRDATTYVGTVRLHTNSARGVWRVQSITVNDKGQNLKI
                     YYANDPLLANAIFRVK"
     gene            5566..>6367
                     /locus_tag="VNN25_15305"
     CDS             5566..>6367
                     /locus_tag="VNN25_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92949.1"
                     /translation="MRRRVQISFLSAFLTLLAIAFAAALVVRARSYSGRTTTESPASA
                     PTAAGTSAARSSRRTDGLSGDEPLPAGVTASTTRDSAETNARAAARAKRFRELLALGA
                     PKLDVPVSPVQTPVKTVPVPVVAKAQTPVSRPTQAASNAFRGSGVSGSAGNGGQPPSG
                     NKANTGTNDPKDPNSDTVPPQLISIAFDPPQVQDGGDTTLIVTATDDLSGIRGISGTL
                     TSPNGKAGQGFAQQRDGETMRYVSRIHIPEKAEEGIWKITFLTMSDMAT"
BASE COUNT         1235 a         2026 c         1955 g         1151 t
ORIGIN      
        1 gacgtcacgt actaccgcga acggctccgc gagaaacgct acgccgtcga tcaggaagca
       61 ttacgcgagt acttcccgac tatgccgtgc gtgaagtgga tgctcgacat cacggagagg
      121 ctgtacggca ttcaattccg cgaggccatc gtccccgtct ggcacgacga cgtcatctac
      181 tacgacgtcg atgatgtcgt tactggcgag cgcatcggcg gtatctacct cgatctctat
      241 cctcgagacg acaaatacaa gcatgccgcc gcctggccgg ttcgcggcgt gtcgcgcaga
      301 actgcgcgaa agccgatcag cgtcctggtg acgaacttca accggcaggg gctgacgcac
      361 agcgaagtcg agaccctgct tcacgagttc gggcacgtga tgcacggcgt cctgtcgcgt
      421 accgagtaca accagcactc cggcaccagc gtggaacggg acttcgtcga ggcgccttcg
      481 cagatgtacg aagagtgggc gagccggatg gagagtctgg ccctgatgcg caaccactgc
      541 acgacctgcc ctctggtcga cgagtcgctc gtgcagcgca tccgtgccgc gaaaaaattt
      601 ggctccggca tcgattacgg ccggcagctt ttgtatgcga gctatgacat ggcgttggcg
      661 ggcgtcggga atcgggggtc gggagtcaga gaacaagctg cccaagcctc ggctccgaca
      721 cccgactccc gaaactcgac ccccgacagc ccatcgctcg agctctggag gaacatggaa
      781 ggctccacgc ccatgggcac catcgacggc accgcctttc ccggcacgtt cgaccacatt
      841 gccagcggct atgcggccgg gtactacggc tacatgtggg cgaaagttat cgcgctcgat
      901 ctcatctccg ccttcggcgg cgatctcatg aacgcggcga cggggcggcg tttccgtgag
      961 ctgatcctgg cccgtggaag cgaagagccg gcgcgcgatc tggtggagca attcctgggg
     1021 cggccggtct ccagcgaagc cttcttcgct gagattcgtg gcgaatagac ttattcgcac
     1081 caatagtgta aggtgtgagt ccttgaggaa ccttcctcaa ccttcaggcc gcctgagctg
     1141 agtcagcgct tctgaactca atcttcttag caaactcacc tttcctccgt gcgtgcgaag
     1201 ctcgcccgct ggaagcggtc aggagtaaca accccatgaa agtctatttt ggaaatctgc
     1261 ccaaagatat cgatgacgcg aagcttgccg agctcgtcaa gccgttcgga accccggcga
     1321 cggcggaagt cgtgaacgac cgcgtcaccg gcctgtcgaa gggcttcgca ttcgtcgagt
     1381 ttgccaatga cgcagaagca caggccacga tcaaaggtct cgatggtacc gatgtgaatg
     1441 gccaggctgt tgtagtcaat gaagcgcgct ccaaaaagga cgcgccgaaa cctgccccgg
     1501 cagttcaaca ctaagaagga gcttgtccca tgacgaacgt tttgaaaccg aagcgcgctg
     1561 cgaaagtcac tgtctcagaa agcaatatcc ggaaggctgc tgctcgcctg agccagcgtc
     1621 ctctggtgtc gcctgaggtg cagtacatcc agcgggtcct tggtgccacg gcaacacagg
     1681 atgcggtcga tgagaaagtc atcgccgtcc gcaaactgcc gtgggcaagc atcgtcattc
     1741 cggagtagcc aaacgccggg cctggcccgc gtgtcacccg ctccgttccc cgagcgtccg
     1801 cgccgatacc ggcgcgcggc gcttttcggg ctcgattaac gaaacgcgct gatgtcgacc
     1861 tctgcgttcc ccgtcgccag tgcgtgaatg acgatcccgt tcgcgtcgag cgtcatcgag
     1921 cgcagctcga cgccgtcgac gtagccgcgc atcacgacgc cgggcgcgag tggccgtgtc
     1981 actgcgctgg cgatcgcgct ccggattgaa tcgatctgcg gcgccaccga ccagtgcgca
     2041 gtcttggcca gctgtgcccg cagcgactcg tgcgccagcc ggtcggcgat gcggacgaaa
     2101 agattcttcc gcttcgaatc gagcgtgtac tcgaggttct gcagcgaaat cgtgcgcgtg
     2161 gcagcgtcga acaccggcgc cgcgtccagg ttgaccggac cgttgtagtg gcggagcctg
     2221 gactcgcggt agtcgacgat caattcgatc gacacgcggc cgtccgcggc aggagcgatg
     2281 cggatcgaat cgacggccac tccttcgtat ttgcgcttcc cgaagttctc ggtcatcacg
     2341 cggctcgctt cgtcgtagga gagctcgacg tcgaacggaa tgcggatgcc gccggtgctc
     2401 gcaggggaca cacgcaacgg tggcagcggt ttctgaacta ctggcggctt ctctccgacg
     2461 atgactcgtg ttcgtgcacg gagtgtgagc gtactggtga ccgagagtcc cgtcccagtg
     2521 atcggtccga gcgacacctc ggcgggctcc atcaccagcc acattcgcga cgcaatctct
     2581 accggcgtct ggagcgagga ccacacctga cttgccgcag gacggatgtt cgtcagcttc
     2641 ggcgtgttcg cgtcgatcgt cttcagcacc tgccggagtt ggtctttgac gatcggcgcg
     2701 atcttccagt cggtgatgtc gatgttcgcg aacgtcacct gacagcggtt ggacgagaaa
     2761 tcgaccggca tcgcccgcgt cgtcgaacgg aggcgccagt tggcatccca ctccaggtgc
     2821 gaggagagcg agatccaggc atcgcgcatc ggctcgccga acccgcacga aaggcacggc
     2881 cacatcacga tctcatcgct gaacggcttc ttcgtcctgc ggcagccttc gagggcgaag
     2941 tgcatggtcg tggttgcgtg gattccgccg ccgatcgcgt tcaggacgat cggatcgcgg
     3001 acgacgcgat acttcatccc gaactgtttc agcggatcga gctcgaacgc gtcggtcttc
     3061 gaaaggcttt tcggtacctg tgattcgagc tggggcagga gcggagcgag cgaggccttg
     3121 atcggtacga cgatcgtcga cagctcggca ggcggtgccg cagctggcgg cccgaatgcc
     3181 tgcggtaccg tttgcgcgag cgccggccag caagcgagag acaggatcag gagcagggga
     3241 gagcgcatag tttgttgttt tgttgacctg tgacgcgttg cagggttgct actttgcctg
     3301 acggtcgatt ggcttctgaa accaatgctt cgatgcacga atgataaagt cctctatatc
     3361 caacgaccga taacgaaaaa agaaccgagc gccgtgagct tggcaaccag gcaacccggc
     3421 aaccagctaa ccaaccaatg tccgacacca ccacccgcgg catccgcatt caggtcgaga
     3481 gcatctatgt cgaagagcga agcgaccctc aggagagcta ctttttcttc gcctaccacg
     3541 tcgtaatatc aaatgtcgga agcgagaccg cccaactcat gagccgcgag tggatcatca
     3601 ccgactccga tggcaacgtc gagcgcgtcg aaggcgacgg cgtcgtcggc gaacagccgc
     3661 tcctcgctcc gggccaggtc ttcgaataca cgagtttctg cccattgcgg acgtcgatgg
     3721 gctccatgca gggcagctat ctcatgcggc cgccgggcgg tgaatctttc cgggcggaaa
     3781 tcgcggcctt cactctcgct gttccgggcg ttgtcaactg aggttcatcc gtgcgacggc
     3841 gagtccagat ctccttccta tccgcgtttc tgacgctcct cacggttgca ttcgcggccg
     3901 ccctggtcgt gcgagtccga tcctactcgg ggccgatgac cacggaatcg tccgcctcgg
     3961 cggcgaccgc ggcggggacg cgcgcggctg cgcggtcgaa tcgcaggaca gacggtctct
     4021 ccggcgacga tccccttccc accggctcga ctgtgagcac tcaaggtggt tcgacggcat
     4081 cgaacgcaac cgccgctgcc gcgcgcgcaa aacgcttcca ggagctcctc gcactcggcg
     4141 cgccgaaact ggatgctccg gtctcgcccg tccaggcgcc cgtgaaaata gttcccgtac
     4201 cggtcgtggc gaaggtgcaa acgccggtca gccggccccc gcaggcggcc gcgaatgcgt
     4261 tccgaggatc gggggcatcc gggtcggcag ggagtaacgg cccgccgtca tccaacaaca
     4321 agccgaatac cccaaccaac gacccgaagg atccaaactc ggataccgtt ccgccgcagc
     4381 tcatttcgat tgcgttcgat ccacctcagg tgtcggacgg tggcgacacc actctcgtcg
     4441 ttaatgccac ggacgacctc tccggcattc gcgggatctc cggcacgctg accagcccga
     4501 atggcaaagc ggtgcagggt ttcgcacagc aacgcgaggg cgagaccacg cgctacgtga
     4561 gccgcattca cattccggag aaagccgaag agggcttctg gaagatcacc ttcctgacca
     4621 tgagcgacat ggccacgaac tccgtgacgc tcagcgccgg gcaggggact ctgccgccga
     4681 ccgcggtgct gaaggtgacc tcatcggcgt ccgactcgac tgctccgacg ctgcgcaacg
     4741 tctggctggc gcgtggcgcg atgcgtgccg gcgaacacaa cacgctctac gtcgacgccc
     4801 aggacgacaa gtcgggagtc catctcgtgg ccgccgtctt cctcagccct tccaagctgg
     4861 cgcgtttcgg cgtcggctgt cacaccggcg cggacaatgt ttgggagtgt gatgtgtttc
     4921 cgcctacgtg cctcgactgc ggcgactgga ctctcgagca ggtgcagttg caggacaacg
     4981 ccaacaactt cgccacgatt cgttcggaga atccggccgt gcagtcagtc cggctcaaca
     5041 tcaccggcga tacctgcgac aaccagccgc ccgtactgca gggactggca ctgagccgca
     5101 cggagatcgt gatggggcgc gacgagccgg tcatcgagtt cgtggcgcag gtttccgacg
     5161 acagttgcgg catcggcggc gtcagcgggc agatcgtcgg cccgggagga aactccagcg
     5221 gcaccttctt ctcgctccag cagcgggacg cgaccaccta cgtaggaacg gtccgccttc
     5281 acaccaactc ggcccgcggc gtgtggcgtg tgcagtcgat caccgtcaac gacaaaggac
     5341 agaacctgaa gatctactac gcgaacgatc cgttgctggc gaacgcgatc ttcagggtga
     5401 aatgaggtca attgacaatt gacaattgat agcgaactac gcgaacgacc cgctgctggc
     5461 ggtgttcggg gcgaagtagc cggacacatc gccttcgttg tcaattgtcc attatcaatt
     5521 gtcaattact tccgtccggg ttgttgtttg atggaaggct acttagtgcg acggcgagtc
     5581 cagatctcct tcctctccgc gtttctgacg ctcctcgcga ttgcgttcgc ggccgccttg
     5641 gtcgttcgcg cccgttccta ctccggacgg acgaccacgg aatcgcccgc gtctgcgccg
     5701 accgctgcgg ggacgagcgc ggcacgatcg agtcgcagga cggacggtct ctccggcgac
     5761 gagccgcttc ccgccggggt gacagcgagc actacgcggg attcggcgga aacgaacgca
     5821 cgtgctgctg cgcgcgcaaa acgtttccgc gagctcctcg cccttggcgc gccgaaactc
     5881 gacgttccgg tctctcccgt ccagacgccc gtgaaaacgg ttcccgtacc ggtggtggcg
     5941 aaggcgcaaa cgccggtcag ccggccgacg caggcggcct cgaatgcgtt ccgaggatcg
     6001 ggggtatccg ggtcggccgg gaatggagga cagccgccct ccggcaacaa agcgaacacc
     6061 ggcaccaacg acccgaagga tccgaactca gataccgttc cgccgcagct catttcgatt
     6121 gcgttcgatc cgccccaggt gcaggacggt ggcgacacca ctctcatcgt caccgccacc
     6181 gacgacctct ccggcattcg cgggatctcc ggcacgctga ccagcccgaa cggcaaagcg
     6241 gggcagggtt tcgcacagca acgcgacggc gagaccatgc gctacgtgag ccgcatccac
     6301 attccggaga aagcagaaga aggcatctgg aagatcacct tcctgaccat gagcgacatg
     6361 gctacga
//
LOCUS       DATLVW010000510         3900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_104633, whole genome shotgun sequence.
ACCESSION   DATLVW010000510 DATLVW010000000
VERSION     DATLVW010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104633"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(773..1939)
                     /gene="metK"
                     /locus_tag="VNN25_15310"
     CDS             complement(773..1939)
                     /gene="metK"
                     /locus_tag="VNN25_15310"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXH92950.1"
                     /translation="MSIHRTLFTSESVTEGHPDKIADQISDAILDAVLAEDPLGRVAC
                     ETLVTTGLAMISGEITTKTYVDFPSIVRNTIKEIGYTRAKYGFDSETCAVISSIDPQS
                     PDIAQGVDTGGAGDQGLMFGFACRETPELMPLPIMIAHKLARRLSEVRKAGELDFLRP
                     DGKSQVTIEYEGKRPIRAAAIVVSTQHSPSIGHGDLSEAIIEKIIRPVVPPELLDERT
                     KFHINPTGRFVTGGPQGDTGLTGRKIIVDTYGGRGRHGGGAFSGKDPTKVDRSACYMA
                     RYIAKNIVASGLADEVEVQLAYAIGVADPVSVYVDTFGTEKISQERIIELVRENFLMT
                     PRGINESLKLRRPIFKKTAAYGHFGRTEPEFTWEATDKAETLRRAAGVAEAVTV"
     gene            2027..3724
                     /locus_tag="VNN25_15315"
     CDS             2027..3724
                     /locus_tag="VNN25_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXH92951.1"
                     /translation="MLKPRNIAAYRDLLVLFTKYGRKDFHLSFDSDEILVSDDPASEI
                     EPDVARRAEAFASALKGMGPTYVKFGQVLSTRPDIVPPEYIVALESLQDDVEPFSFAE
                     VERIVEEDLKVRISKAFESFESTPLAAASLGQVHRAVLRDGREVVVKVQRPNVREQVK
                     HDLEVFAEIATTIEQHSDVGRKMNLIGAIEQARISLMNELNYEQEARNTEILRKNLAE
                     FPQIYIPTVIHDMTSSRVLTSEHIRGRKVSKLTPLQVIDHDYADLASALTRAYLKQIC
                     VDGIWHSDPHPGNVFVRDLEDNQSEVVLLDFGMVSRVSREFQDEIIKLLLAVSSNRGA
                     EAADACVRVSEVQERFDAMRFVREISALVATFHDVDVRQINIGRLIFRVIGVANSNEL
                     KVPAELAMLAKTLLHLDGVTRRLDPDYDPHTVIRDYAERLMVQKLAQKFAPRNFYPAL
                     LDLNQLMLDLPHRAREIIDLTAAGRLAFHLNLTQAEQFLAGIHKIANRITVGVIIAAL
                     LIASSMMMRVPTHAQLFGYPAIAVTGYLLAAIAGIYLIVTTLVTDRKDEQKAKIQRR"
BASE COUNT          750 a         1224 c         1150 g          776 t
ORIGIN      
        1 gatcgtggga gtagtagaaa cgtgtcggac tggagagtcc gacctacgtt cctcccaagt
       61 ccgacctacg ttccttccga gtccggccta cgttccttcc gagtccggcc tacgttcgtt
      121 ccagcgcttt cacaggctgt agacgcgagg cgcgaatcga cgtcaactac cgtgacttca
      181 cgtaatgatc ttcgccactc gtacggcgca acgtaggtcg gactctctag tccgacgcct
      241 cttttcgaaa aacgtgtcgg actggagagt ccgacctacg ttccttccga gtcgacctac
      301 gttcctcccg agtccggctt acgttccttc cgagtccgac ctacgttcgt tccagcgctt
      361 tcacaggctg tagacgcgac gcgcgaatcg acgtcaacta ctgtgacctt caagtaatga
      421 tcttcgccac acgtaaggcg caacgtaggt cggactctct agtccgacgc cccttttcga
      481 aaaacgcgtc ggactggaga gtccgaccta cgttccttcc gatcgattca ttcacgtcgg
      541 agccagggat aatcatcggg cgaagaactc ttccgctgga tctcgaagag gatgtgctgc
      601 atcacaagct ggcacacttt ccgctatcca caccatcatc ccgactcgct cgggagacgc
      661 tggcgaagtt tggagcgcgc cgataatccc tgaggggtcg gcgcgcttcg tgttttgagc
      721 acaaaaaggc cgcgacatgc gcggcctttt gttgttttga agagatcgag acttagacgg
      781 taaccgcttc ggcgacgccc gcggctcgac gcagcgtctc cgccttgtcg gtcgcttccc
      841 aggtgaactc cggctccgtg cggccgaagt ggccgtatgc cgcggtcttc ttgaagatgg
      901 gacggcgcag tttgagcgat tcgttgattc cgcgcggtgt catcaggaag ttctcgcgga
      961 cgagctcgat gatcctctcc tgcgagatct tctccgttcc gaacgtgtcg acgtagaccg
     1021 acaccggatc ggccacgccg atcgcgtagg cgagctgcac ctccacttcg tcagccagac
     1081 ccgacgccac gatgttcttg gcgatgtagc gggccatgta gcaggccgaa cggtcgacct
     1141 tcgtgggatc tttgcccgag aacgcgccgc cgccgtggcg tccgcggcca ccgtaggtat
     1201 cgacgatgat cttgcggccg gtcaggcccg tgtcgccctg cggtccaccg gttacgaaac
     1261 gaccggtggg attgatgtga aactttgtcc gttcgtcgag aagctcggga ggaaccacgg
     1321 gccggatgat cttctcgatg atggcctcgc tcaggtcacc gtgaccgatg ctcggcgagt
     1381 gctgcgtcga gacgacgatg gctgcggcgc ggatcggccg ctttccttca tactcgatcg
     1441 tgacctgcga cttgccgtcg gggcgaagga agtcgagctc gcccgctttc ctcacctcgg
     1501 acagccggcg cgctagcttg tgcgcgatca tgatcggcag cggcatcaac tccggcgtct
     1561 cgcggcaagc gaagccgaac atgaggccct gatcgcccgc accgcccgta tcaacgccct
     1621 gggcgatgtc cggtgattgc ggatcgatcg aggagatcac ggcgcaagtc tcgctgtcga
     1681 agccgtactt ggcgcgcgtg tatccgatct ctttgatggt gttgcgcacg atcgacggga
     1741 agtcgacgta cgtcttcgtg gtgatctcgc cggagatcat cgccagaccg gtcgtgacga
     1801 gcgtttcgca ggcgacgcgg ccgagcggat cttcggccag aacggcgtcg agaatcgcgt
     1861 ccgagatctg gtccgcaatc ttgtccggat ggccctcggt gacagactcc gaggtgaaca
     1921 gggttctatg gatagacatc gtgaaaagct cctaattgga ttctggtaat cctagcatgc
     1981 cggcccgatc agagtcaggc gcgcagaatg caaactcaac ggcagaatgc tgaagccacg
     2041 gaacatcgcc gcctaccgcg atctgctcgt cctctttaca aaatatggcc gcaaagactt
     2101 ccacctctcg ttcgattccg acgagattct ggtcagcgac gaccctgcct cggagatcga
     2161 gcccgacgtc gcccgccgcg ccgaggcctt tgccagcgca ctcaagggga tgggaccgac
     2221 gtacgtcaag ttcggccagg tcctctcgac acggcccgac atcgtccctc ccgagtacat
     2281 cgtcgccctc gaatcgctgc aggatgacgt cgagccgttt tcattcgccg aagtcgagcg
     2341 gatcgtcgaa gaggatctga aggtccgcat ctccaaggcc ttcgaatcgt tcgagtccac
     2401 tcccctcgcc gccgcctccc tcggacaggt tcaccgcgcc gtgctgcggg acggccgcga
     2461 agtcgtggtc aaggtgcagc gtcccaatgt gcgcgaacag gtcaagcacg atctcgaagt
     2521 cttcgccgag atcgcaacca cgatcgagca gcacagtgac gtcggccgga agatgaacct
     2581 cattggcgcg atcgaacaag cgcgcatctc gctgatgaac gagctgaact acgaacagga
     2641 ggcgcgcaat accgagatcc tgcgcaagaa tctggcggag ttccctcaga tctatatccc
     2701 caccgtcatt catgacatga ccagctcgcg cgtcctgacg agcgagcaca tacgcgggcg
     2761 gaaggtctcg aagctgacgc cgctgcaggt catcgaccac gactacgccg acctcgcctc
     2821 ggcgctgacg cgtgcctatc tcaaacagat ctgcgtcgac gggatctggc actccgatcc
     2881 gcatcccggc aacgtcttcg tgcgcgatct cgaagataac cagtccgagg tcgtcctgct
     2941 cgacttcggg atggtgagcc gcgtcagccg cgagtttcag gacgagatca tcaaactgct
     3001 gctggctgtg tcgtcgaatc gcggcgccga ggcggcggat gcgtgcgtgc gggtgtcgga
     3061 agtgcaggag cgcttcgacg ccatgcgctt cgtgcgcgag atctcggcgc tggtggcgac
     3121 gtttcacgac gtcgatgtcc gtcagatcaa catcggacgc ctcatcttcc gcgtcatcgg
     3181 cgtcgccaac agcaacgagt tgaaggtgcc ggcggagctg gccatgctgg ccaagacgct
     3241 gctgcatctc gacggcgtca cgcggcggct cgatcccgat tacgacccgc acacggtgat
     3301 ccgcgattat gccgagcggc tgatggtgca gaagctcgcc cagaagttcg cgccgcgcaa
     3361 cttctaccct gccctgctcg atctcaatca attgatgctc gacctgccac accgcgcacg
     3421 cgagatcatc gacctcaccg ccgccggccg gctggcgttc cacctcaatc tcacgcaggc
     3481 cgaacagttc ctcgctggca tccacaagat cgccaaccgc atcacggtgg gcgtgatcat
     3541 cgccgcgctg ctgatcgcct cgtcgatgat gatgcgcgtc ccaacacacg cacagctctt
     3601 cggatatccg gcgatcgcgg tgaccggcta ccttctcgcc gcaatcgccg gcatctacct
     3661 catcgtcacg acgctagtca ccgatcggaa ggacgagcag aaggcgaaga ttcagaggag
     3721 atgacgggca gtaagcggca tgaacttcag ggattcacca cagagacact gagagcacag
     3781 aggttctttg tgtcctctgt actactaacc aatagattct tctcctgacg cgaaggtttt
     3841 catgcggcga tgggggtgag ctggctgatg gtggctcgaa gggcggagag gattgcggtg
//
LOCUS       DATLVW010000511         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_557802, whole genome shotgun sequence.
ACCESSION   DATLVW010000511 DATLVW010000000
VERSION     DATLVW010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557802"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..664)
                     /locus_tag="VNN25_15320"
     CDS             complement(47..664)
                     /locus_tag="VNN25_15320"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HXH92952.1"
                     /translation="MPRTSALAVLDFADIPAGVCAVDAILKKAPIAFVRAGTVTRGRY
                     LVVFGGSTASTAESLAEALGTSGEAIIDYTFLPDAHPSLFDAAFGARRKINGSLLIIE
                     TETAASIVRAVEAALKGTPVELIEMRLADEGLAGKGIALMAGTLHDVEYAAILAANGV
                     GVDAPPPRGFSHRLIAAPHEILERDIAAVTRFDIAPLLELDGEAG"
     gene            complement(664..2097)
                     /locus_tag="VNN25_15325"
     CDS             complement(664..2097)
                     /locus_tag="VNN25_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXH92953.1"
                     /translation="MTRLTDQEIEQIARRIAADMGRVAAEQPPAVQAQATGLGVFTTV
                     SDAVSAATIAQKEFVALKLEHRARIIEAMRQSMRENGAMLAKAAFDETGFGRYEDKIV
                     KNRLVTEKTPGLEDLDPIATTGDYGLALIEPAPFGVIGAITPVTNPTSTIICNSIGML
                     AAGNSVVFNVHPYSKSCSMQTIALLNKAIAATGGPPNVICGIATPTIESAQDLMKHPG
                     VRLVVVTGGGEVVKVAMASGKRAICAGPGNPPVVVDETADIDQAARDIVLGASTDNNI
                     ICTDEKETIAVSSIADALIASMGRQGAIVIPRERLSELEKVVFTEMKGPRQKAWIDKN
                     LVGKNANVILAKMGMNVPDSVRLIVVEVDENHPLLWTEQMMPVMPVVRVPDANRAIDL
                     AVQVEGGNRHTAVMHSKNLDNLSRMAKACDCSIFVKNGRSQAGLGLDGEGYCSFTIAS
                     PTGDGLTSPRSFSRWRRCTLVGAFRIT"
     gene            complement(2121..2408)
                     /locus_tag="VNN25_15330"
     CDS             complement(2121..2408)
                     /locus_tag="VNN25_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HXH92954.1"
                     /translation="MQLARVVGTVVATRKEDSMQGLKLLVVRPLDEEGRESGNAIVSA
                     DAVGAGPDEIVLIAAGSSARQTAVTDKRPVDSVILAIVDSWYVGDAVKYKK"
     gene            complement(2423..2734)
                     /locus_tag="VNN25_15335"
     CDS             complement(2423..2734)
                     /locus_tag="VNN25_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HXH92955.1"
                     /translation="MTDALGMIETRSFPAVVEAADAAVKAARVELVSYEKTGGGYTTI
                     VIRGDVAAVKAACDAAQTAAGRVGDVVTVHVIARPHDQVDVIMPLGRTSAPAVPARSK
                     K"
     gene            complement(2887..3510)
                     /locus_tag="VNN25_15340"
     CDS             complement(2887..3510)
                     /locus_tag="VNN25_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92956.1"
                     /translation="MAELRCYVFIDSLQPQFASFLGTVAQGFLPVPGQAALFVEIAPG
                     MDIMRVTDVALKATDVKPGMMIIERVYGMLELHADSQADVRQAGQAILDMLNLKETDR
                     IKPEITSSQVIRNISDYHTQLINRTRHGDMIIPGQSLYVMEVAPAGYAAIAANEAEKA
                     ANINILEVRAFGSFGRVYLGGEERDIEVGYRAAEQAIESIAGRAGRP"
     gene            complement(3514..4200)
                     /gene="deoC"
                     /locus_tag="VNN25_15345"
     CDS             complement(3514..4200)
                     /gene="deoC"
                     /locus_tag="VNN25_15345"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HXH92957.1"
                     /translation="MPPDQLVKLIDHTLLKPEATAEDIEKLCQEAAKFGFYSVCVNPT
                     WVAKAKALLRGTKVKVCAVVGFPLGAQSSEIKTLEARRALREGAQEIDMVINIGALKS
                     KDDNLVARDIRGVVEVCDDARAVCKVILETALLTDEEKVRGCLLSMKAHAGFVKTSTG
                     FSKAGATVEDIALMARTVAPRKLGVKASGGVRTYADVIKMVQAGATRVGSSNSVKILE
                     EATQMASGKR"
BASE COUNT          701 a         1336 c         1354 g          855 t
ORIGIN      
        1 gtcacgatgc acaacacaac ctgaccgatg acgcgcccga ggagcatcag ccggcctccc
       61 cgtcgagctc gaggagaggg gcgatgtcga atcgcgtgac cgcggcgatg tcgcgctcca
      121 ggatctcgtg cggcgcggcg atcaggcgat gcgagaaacc gcgcggcggc ggcgcatcga
      181 ctccgactcc gttggccgcc aggatcgccg cgtattcgac gtcgtgcagc gttccggcca
      241 tcagcgcgat gcccttgcca gccagccctt catcggcgag gcgcatctcg atcagctcga
      301 ccggcgttcc tttgagagcc gcttccaccg cgcgaacgat cgaagcggcg gtctcggtct
      361 cgatgatgag aagcgagccg ttaatcttcc ggcgcgcacc gaaggctgcg tcgaagagcg
      421 acggatgcgc atccggcagg aaggtgtaat cgatgattgc ctcgccgctc gttcccagcg
      481 cctccgccag cgactcggcg gtcgacgcgg tcgacccacc gaatacgacg aggtagcggc
      541 cgcgagtcac ggttccggcg cgcacgaacg caatcggcgc ctttttcagg atggcgtcga
      601 ccgcgcagac gcctgccggg atgtccgcga agtcgaggac ggcaagagca gaggtcctcg
      661 gcatcacgta atcctgaacg cgccaacgag cgtgcagcgg cgccagcgcg agaatgaccg
      721 cggactggtc agaccgtcgc cggtggggct ggcgatggtg aaggagcagt agccttcgcc
      781 gtcgagcccg aggccggctt gggaacggcc gttcttgacg aagatcgagc agtcacacgc
      841 tttcgccatc cggctcagat tgtcgagatt cttcgagtgc atcaccgcgg tgtggcggtt
      901 tccgccttcg acctgcacag caagatcgat ggcgcgattc gcatcaggaa cgcgaacgac
      961 cggcatgacc ggcatcatct gctcggtcca gagcagcgga tgattctcat cgacctcgac
     1021 gacgatgagg cgaaccgagt cgggaacgtt catccccatc ttggcgagga tcacgttggc
     1081 gttcttgccg acgaggttct tgtcgatcca cgctttctgc cggggaccct tcatctcggt
     1141 gaagacgacc ttctccagtt cggagagccg ctcgcgcggg atgacgatgg cgccctggcg
     1201 tcccatggag gcgatgagcg cgtcggcgat cgacgagacg gcgatcgttt ccttctcgtc
     1261 ggtgcagatg atgttgttgt cggtcgaggc gccgagaacg atgtcgcgcg cggcctggtc
     1321 gatgtccgcg gtctcgtcca ccacgacagg cggattgccc gggccggcgc agatggcgcg
     1381 cttgccgctg gccatggcga ctttgacgac ctctccccca ccggtcacga cgacgaggcg
     1441 gacgccggga tgcttcatca gatcctgagc cgattcgatc gtcggggtgg caatgccgca
     1501 gatgacattg ggaggaccgc ccgtggccgc gatggccttg ttcaggagag cgatcgtctg
     1561 catgctgcag ctcttcgagt acggatggac attgaatacg acgctgttgc cggcggcgag
     1621 catcccgatc gagttgcaga tgatcgtgga cgttggattg gtgaccggtg tgatcgcgcc
     1681 gatgacgccg aacggagcgg gctcgatgag cgcgagaccg tagtcgccgg tcgttgcaat
     1741 cggatcgaga tcttccagcc ccggcgtctt ctcggtgacg aggcggttct tgacgatctt
     1801 gtcttcatac cgcccgaatc ctgtctcgtc gaacgctgcc ttcgccagca tcgcgccgtt
     1861 ttcgcgcatc gattgccgca tcgcttcgat gatgcgggcg cgatgctcga gcttgagcgc
     1921 gacgaactcc ttctgcgcga tggtggcggc gctcacggca tccgagaccg tggtgaagac
     1981 gcccagtcca gtcgcttgcg cctgaacggc cggcggctgt tccgcggcaa cgcggcccat
     2041 gtcagcggcg atgcgccgcg cgatctgttc gatttcctga tcggtcaggc gagtcattgg
     2101 ctagtcactt cccgggctac tcacttcttg tacttcacgg catcgcccac gtaccagcta
     2161 tcgacgatgg ccaggatcac cgagtcgacg gggcgcttgt ccgtgaccgc ggtttgccgc
     2221 gcggagctgc cggcggcaat cagtacgatc tcgtccggac cggctcccac ggcgtcggca
     2281 gacacgatgg cgttaccgct ctcgcggcct tcttcgtcca aagggcgcac gaccaggagc
     2341 ttgagtccct gcatgctgtc ctctttccgc gtggcgacga cggtaccgac gactctcgcg
     2401 agttgcatgg acgtgggtcc tgctacttct tcgagcgagc gggtactgcc ggtgccgagg
     2461 tgcggccgag cggcatgatg acgtcgacct ggtcgtgcgg ccgcgcgatc acgtgcaccg
     2521 tgaccacgtc gccgacgcgt cctgccgccg tctgcgcggc atcgcacgcc gctttcaccg
     2581 cggcaacgtc gccacgaatg acgatcgtcg tgtagccacc acctgtcttt tcataggaga
     2641 cgagctccac gcgcgctgcc ttcaccgctg cgtcagcggc ttcgaccacc gccggaaaag
     2701 acctcgtttc gatcatgccc agtgcgtctg tcatgcgtcc tccttaatca gaaggcagat
     2761 ggcagaaggc agaagggtgc aaagtcggga accctttcag ctttctgtct tctggtttct
     2821 tccttctggt tctgcctttc gttttctgtc ttgaacttct gctctctgcc ttctgctttc
     2881 tacttttcag ggccgtccgg cgcgtccggc gatcgattcg atcgcctgct ccgccgcccg
     2941 gtatccgact tcgatgtcgc gttcttcgcc gccgaggtag acgcgcccga aactgccaaa
     3001 cgcccgtacc tcgaggatat tgatgttggc tgctttctcg gcttcgttcg cagcgatcgc
     3061 cgcgtagccg gcgggtgcca cttccattac atacagcgac tgccccggga tgatcatgtc
     3121 gccgtggcgc gtcctgttga tgagctgcgt gtgatagtcg gagatgttgc ggatcacctg
     3181 gctggacgtg atctccggct tgatgcggtc cgtctccttc aaattcagca tgtcgagaat
     3241 ggcctgtccg gcctgacgaa cgtcggcttg tgaatcggcg tgcagctcga gcatgccgta
     3301 gacgcgctcg atgatcatca tccctggctt gacatcggtc gccttcagcg cgacgtcggt
     3361 gacgcgcatg atgtccatgc ccggcgcgat ctcgacgaag agcgccgcct gccccggcac
     3421 cggaaggaag ccctgcgcga cggttccgag gaacgacgcg aactgcggct gcaggctgtc
     3481 aatgaatacg tagcagcgaa gctcggccaa ctttcacctc ttccccgagg ccatctgggt
     3541 ggcctcttcc agaatcttga cactattgga gctgccgacg cgcgttgcgc ctgcctgcac
     3601 catcttgatt acatcggcat aggtacggac gccgccggaa gctttgacgc cgagtttgcg
     3661 cggggccacg gtgcgtgcca tgagggcgat gtcctcgacc gtcgcacccg ccttcgagaa
     3721 gccggtcgag gtcttgacga agccggcatg cgctttcatc gagagcaggc agccgcggac
     3781 tttttcttca tcggtgagca gggcggtctc gaggatgact ttgcagacgg ctcgtgcgtc
     3841 atcgcagact tccacgacgc cgcggatgtc gcgggccact aggttgtcgt ccttgctctt
     3901 cagtgcgccg atgttgatga ccatgtcgat ttcctgcgcg ccttcgcgca gtgcgcgccg
     3961 tgcctcgagc gtcttgatct ccgaggactg ggcgccgagg ggaaagccga ctacggcgca
     4021 gactttgacc ttcgtcccgc gaagcagcgc cttcgccttc gcaacccagg tgggattcac
     4081 gcagaccgag tagaagccga atttcgccgc ttcctgacag agtttctcga tgtcttcagc
     4141 ggtagcttcc ggcttgagca gggtgtgatc gatcagtttg acgagttgat cgggaggaat
     4201 gacgagcgtc gcgggcgagg cggagccacc gggctggaga agctgg
//
LOCUS       DATLVW010000512        12905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_557895, whole genome shotgun sequence.
ACCESSION   DATLVW010000512 DATLVW010000000
VERSION     DATLVW010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557895"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VNN25_15350"
     CDS             complement(<1..899)
                     /locus_tag="VNN25_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92958.1"
                     /translation="MKIAIAGTRGVPARYGGFETFAAELSARLVARGHEVAVYCRSGS
                     GEWGVGSGNGSRPTPAPGELGARDGGEAETSPTPHSPHPTPQVQRIVLPALRHKYFET
                     VSHTFLSALDALRRDFDVILLCNAANAFVLPLLRAARIPVAINVDGIERRRRKWNVMG
                     KAVYAIGESFSALFASRVVADAEVIADYYRGRYGIEPVMIPYGAEFPIEEDSDVLDRL
                     GVQSGNYILYVSRFEPENNPLEVVRAYEGVGSGEWGVGEETAGIADRELGVGVRGNIL
                     GAGTRRSSDPSLPPPPPPTPPPPP"
     gene            complement(896..1975)
                     /locus_tag="VNN25_15355"
     CDS             complement(896..1975)
                     /locus_tag="VNN25_15355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH92959.1"
                     /translation="MERGLTNGLVSVIIVSWNSARFLERCLSALAQQTHRSIELLHID
                     NASGDDSIAMVRSAFPFARQITNDTNRGFSAAVNQGVRLANGEFVLLLNPDAFLEPEY
                     VERLVAALDRAGETFGMATGKLLQAESGLIDSMGIRMTRSGRHFDVDQGRGVGSRSEK
                     VLAHSRSQSRPHSQPITNQQDSLPGSAPTPDPRLATPAREVFGVSGAAAMYRMSFIRD
                     VAIGGEFLDEDFFAFREDADVAWRGQLFGWRAIYVPDAVGHHVRTVTPEKRGKLSADI
                     NMHGVKNRFLLRLKNEGVYLALRNAPFELGRDLVVIIAALTIERSSLPALSWLWKNRR
                     RIMEKRRAIQSRRRVSDRQIAGWFR"
     gene            complement(1960..2964)
                     /locus_tag="VNN25_15360"
     CDS             complement(1960..2964)
                     /locus_tag="VNN25_15360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH92960.1"
                     /translation="MEKATRKLSSDQPAPMLRGIATGPLDLSIAIVTWNSERWIERCL
                     QSVRAACGRLEYEVVVYDNASSDATLAAMGKDVEVIRGAVNDGFAAGVNRVASRTSGR
                     YLFLLNPDCELSPRSLEILVGFLDENPAIAAAAPLLSDEAGESQREFQLRRLPTLATL
                     ISEAFGIDKLFPDNPMTARYRYRELELTHPQPVEQPAAAALLVRREVFTEVGPLDERF
                     TPAWFEDVDYCRRLAAAGKTIFVVPAARARHFGGASLEHVPFAEFIALWYRNMWLYAR
                     KWHTPGEAEVLRWAIVFGMLLRIPLALAGLANRHLGRLHAARAYAGVMRKAFARWSGV
                     "
     gene            complement(2940..4046)
                     /locus_tag="VNN25_15365"
     CDS             complement(2940..4046)
                     /locus_tag="VNN25_15365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH92961.1"
                     /translation="MRAVNIVVHTPDVVGERMAGPGIRAWHLAGELAKHFTVTLVCKR
                     EGELPGAASFRVAARDSAEANARIREAEVLIGQPARGFRRQRRGQRIVYDLFDPVLLE
                     LHEMYGRHPSMRQRVHVEAERWRVRRAVATGDLLVVATKKQRELYRGAQGPIIEVPFG
                     VEDFDAAAPQDRENIVLWGGGTWEWLDPATAVEATIAVNRRGIDCRLLFLGRSRPNQH
                     TVDRRREDRLDEWITAGGAFVEANDSWMPYNSRLSWLRRSKVAIMLHRPTAEAAYSIR
                     TRLFDAIAAAVPVITTEEGFAAELVAAEELGIVVPPSNAPAVADAIAKLMTDDAFYAR
                     CVSNLIRIQPRFGWAAVTGPLIEAVKEWKRQREN"
     gene            complement(4101..4970)
                     /locus_tag="VNN25_15370"
     CDS             complement(4101..4970)
                     /locus_tag="VNN25_15370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH92962.1"
                     /translation="MTDVSVIVLDIDGGDMLRACLESITSQTLPPKEVIVFDNGSRTP
                     AAERIAFRHALHLFRSETNLGFAGGNNEAYRHATGNYIALVNNDVVLDRDWLATVASA
                     LDNDPKLAAVQTILRRDETTIDGAGIDISDGTFRQIAAGVGSRESGVGAKTKSLVPTP
                     DSRPPTPLWGVSATAALYRREALGRTIFDEQFFAYYEDVDLCARLHEAGWRTEVLPVI
                     KATHRGSQSAPLLLGEALRLRTRNRYFVARRHRGVGRISALLWEDTKLLMKGRSSLQG
                     IVEGLTRRMKDEG"
     gene            complement(4963..6978)
                     /locus_tag="VNN25_15375"
     CDS             complement(4963..6978)
                     /locus_tag="VNN25_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92963.1"
                     /translation="MKLALFIVAALAMPLLVLRAPVLRRCLGTQLGAVIAFAILWAYG
                     RVLLIDVDPYLVFVTLGVIILAIPLAFLAFADDVRWSANRAFLIAAIGYAVMIPFQLL
                     TPIDGDEPYYLMMSESLVRDHDLDLTNQYREIAHSDTGRPDLAPQPGDPRGAHGEQYS
                     RHEPFLPILMAPGYALGGVPGALAVIALFGALLARSTVRFLEDEGIDDATTRALFPLI
                     AFGPPIVFYATRIWPEVPAAFCFLEALRGVRQRRPARWITAIFALVLLKIRFGLIAIV
                     LLARALRTRRQVVIAGAVVAVPLLVAWMITGNPMNTHSWQELIPGGPMSYWRGLFGLV
                     LDGAGGLLFQAPLYVLAIVALARWREMPEGFRLGISASLLYILYLVPRSEWHGGWSPP
                     LRYIVVLMPVLALGSAALWEKVGTGTRALIAGWTLMLVAHGAAFPWRLFHLATGENSI
                     GESLSERWHSDFSRLMPSFIRPNLAATVASVVLVVVLGMVGIARSREWGVGSGVWEFP
                     GGSNLRRILRRASDDSHPPLPTPHARSPRRTMTGIAPLLFAAILLAAFTYGRRPADRV
                     EFEDAHVIHNGGELFPAEWTVSRFLYQGGWVVHAGDTLSFLAKPGPSMLRYQSRGGAT
                     IEIGGHAFVLPPSGAIYGYTTINTGPGGRAQLRCLDGSANLDWMQHD"
     gene            complement(6975..8228)
                     /locus_tag="VNN25_15380"
     CDS             complement(6975..8228)
                     /locus_tag="VNN25_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92964.1"
                     /translation="MLTTPIDAHLPLIVALVIALVLICAWTGRGTRWAAPVQCAVPLL
                     IVAMMTLADERTRLLAYGVIVALAYGAATMAVYASSASAMERRRPAGWLGGVSPPEIA
                     LTVVGIVLLRWIPLHDVRIVRELVVLAGSLALLFAMPRRRDAAGPAGGTPAFLIAVLA
                     VAVATPARAGMVTFLPFALALLVVGLRSLGVSASTSSRVLPSVAIAAVLLLCACFARY
                     SLATVYVAAAIVFLAPLIDRVRPLSYAAVVAIFALWPWSGIIARALPIARNYAPSVGE
                     VQTISHALAANESLPVSVPPHVREAIVTTCGGYTERLRSGHVVGAIEATDQHGRVTVR
                     PIRIGDTADFGFMRREQFFASRNSLPRFSPGEIREYGANAWIVGAGRTTVTCASDIVS
                     LRVVAAGDLHAQSRLAIDSVEYPKR"
     gene            complement(8222..9115)
                     /gene="dapF"
                     /locus_tag="VNN25_15385"
     CDS             complement(8222..9115)
                     /gene="dapF"
                     /locus_tag="VNN25_15385"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXH92965.1"
                     /translation="MQTQASRQDPISFLKMAGGGNDFVVIDNRSGRIDDAAALTRRIC
                     TPHLSVGADGVILIENTGKATFRMRYLNADGSPADFCANGTRCAARFALVNVIAPKKM
                     TIETGAGVVAAEVSDGGLVTLTLPQPHSFQADRPLRVGETVIRGSSIVVGVPHYVVFL
                     RDNLWAQDIVPLGRALRMHPGLQPAGANINFVVVREPGAIEVRTYERGVEAETLSCGS
                     GVVASAVTSALFGKTTSPVKVLTRSGITLEVSFALDGSDVSEVQLRGDARVVYRASMT
                     PDTIEGFDPEWVRNPAENVVC"
     gene            9306..11003
                     /locus_tag="VNN25_15390"
     CDS             9306..11003
                     /locus_tag="VNN25_15390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH92966.1"
                     /translation="MNRLRFTRVTSAILIAALTTGPLAAQQKTPTELPKLVENIDVHV
                     IGVDVVVTDKKGNPVTGLTKDDFRIFENGEEKQISNFYEIDGKVAKPPVTTGTAPATT
                     AAAPAPAPKEEISEQLKRRIIIYIDNLSLAPFNRNRVFKQMKDFVKDVMRPGDEAMLA
                     TYNRSLKVRVPFTRDPVQIQQMLDSIAGESGFGIANKSEAKDVMGRIRDATNYDDALA
                     DARTYASSVDHDLRQSVESINGLLTTLAGVEGKKILILTTEGFQIQPGREVFAMIDDV
                     AKDKGWQSSSTLEAMSYNGAPLIESIAKTANANGITIYAIHAGGLAAGSEGTSADNQQ
                     AISYNVTSAAVSNTTESLQLMAELTGGLATTRTNNFAGAFKNIGRDLDSYYSLGYRAG
                     TERVDRQRTLEVRVNNKALQVRNRQTFVEKSTFQDMSDRVIANLLYRTKSNDLNVRVK
                     VNAPIPADDLFKIPVEIQIPVDNLTLLQQGEAYMGGFSIYVVVGNKDGDMSDVSRKSH
                     QITIPLADFPKSKGRYYTYTLDLMCEPGLNKISIGVVDDVSNISGFDRQQVIAQDLR"
     gene            11067..11282
                     /locus_tag="VNN25_15395"
     CDS             11067..11282
                     /locus_tag="VNN25_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92967.1"
                     /translation="MRNLTLKHWRLIKDRGAEAAREMALLWLVFSALDALLSGRLTLP
                     YLTMNFFGCAMLWLLGIYLEITAKEAQ"
     gene            11279..11443
                     /locus_tag="VNN25_15400"
     CDS             11279..11443
                     /locus_tag="VNN25_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92968.1"
                     /translation="MNPQLYATLPFVMFGGFLLVVGLWSIHKIRKEEASKRAAGFTAS
                     PPESRSHSGK"
     gene            complement(11578..12540)
                     /locus_tag="VNN25_15405"
     CDS             complement(11578..12540)
                     /locus_tag="VNN25_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXH92969.1"
                     /translation="MTTQPLSRHPTFEKIAALQANVERVIRGKSEVVQFCIAALLSKG
                     HILLEDVPGVGKTMLAHALARSLSLSFQRIQFTSDLLPADIVGVTIYNQDAQEFEFVS
                     GPVFTNILLADEINRATPKSQSALLEAMSEGMISIEKKRLALPDPFLVLATQNPIEHV
                     GTYPLPESQLDRFLMKLTIGYPNPTDEKKLLRSGGAQDALEHLEPVLEEQEVRDLQAH
                     VASIHVNESLVDYLMTIVETTRTHAEAALGVSTRGALTYFKACQALAMVNGRDFVIPE
                     DVKRLAGPVLSHRIVMKDRRLLRADGSPEQRFIQRIVSEIPVPR"
     gene            complement(12606..>12905)
                     /locus_tag="VNN25_15410"
     CDS             complement(12606..>12905)
                     /locus_tag="VNN25_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXH92970.1"
                     /translation="VTDVYNGGVIHEENQPAHLLIADTEVCITRCTEEFGNPCFHFCP
                     AQVYEPQPTADGRGKVPFLNFTNCFHCKTCDIMDPYQVITWVPPEGGGGPHYKKL"
BASE COUNT         2566 a         4090 c         4067 g         2182 t
ORIGIN      
        1 gggggggggg ggggggtggg gggggggggg ggggggagag aagggtccga agaccgacga
       61 gtgccggcgc cgaggatgtt gccgcgcact ccgactccca actcccgatc cgcgattccc
      121 gcagtctctt ctcccactcc ccactcccca ctccccaccc cctcataagc cctcaccacc
      181 tccaacggat tgttctccgg ctcgaagcga ctcacgtaga ggatgtagtt accggactgg
      241 acgccgaggc ggtcgaggac atcgctgtcc tcttcgatcg gaaactccgc accgtacggg
      301 atcatgaccg gctcgatgcc gtaacggccg cggtagtagt cggcgatgac ctcggcgtcg
      361 gcgacgacgc gcgaggcgaa gagcgcggag aacgattcgc cgatcgcgta gaccgccttt
      421 cccataacat tccacttgcg ccggcggcgc tcgatgccat cgacattgat cgccaccggg
      481 atccgcgctg cgcgaagaag cgggagcacg aaggcattgg ccgcattgca gagcaggatc
      541 acgtcgaaat cgcgtcgcag cgcgtcgagc gccgaaagaa aggtgtggct gacagtctcg
      601 aagtacttgt ggcgaagggc ggggaggacg atgcgttgga cttggggggt ggggtgtggg
      661 gagtgggggg tgggagaagt ttccgcttca cccccgtcac gggcgcccaa ctcgccgggt
      721 gctggcgttg gcctggaacc gttcccactc cccacccccc actccccact ccccgaacgg
      781 cagtacaccg ctacctcgtg cccacgcgcc accagccgcg ccgacagctc cgcggcgaac
      841 gtctcgaagc cgccgtaacg ggcaggcaca ccgcgcgtgc cagcgatggc gatcttcaac
      901 gaaaccatcc ggcgatctgg cggtcggaga cgcggcgccg gctctgaatc gcgcgtcgtt
      961 tctccatgat ccggcgtcga ttcttccaca gccacgagag cgcgggaagc gagctgcgct
     1021 cgatcgtcag cgcagcgatg atgacgacga gatcgcgtcc gagctcgaac ggcgcgtttc
     1081 gtaacgcgag ataaacccct tcgttcttca ggcgcagcag gaagcggttc ttgacgccgt
     1141 gcatgttgat gtccgcggag agtttgccgc gcttctccgg tgtcacggtg cgcacatgat
     1201 gaccgacggc gtcagggacg tagattgccc gccagccaaa gagctggccg cgccaagcca
     1261 cgtccgcatc ctcacgaaac gcgaagaaat cctcatcgag aaactcgccg ccgatcgcga
     1321 cgtcgcggat gaacgacatc cggtacatcg cggcggcacc ggagacgccg aagacctcgc
     1381 gcgcgggggt cgctagtcgg gggtcggggg tcggagcgga acctggcaag ctgtcttgct
     1441 gattcgtgat cggctgcgaa tgaggccggc tttgcgaacg gctgtgagcc agcacttttt
     1501 cgctccgact cccgaccccc cttccttggt ccacatcgaa atgccgtcct gaccgcgtca
     1561 tccggatgcc catcgaatcg atcagaccac tctccgcctg gagcagcttg ccggtagcca
     1621 tgccaaacgt ttcgccggcg cggtcgaggg ctgcgacgag gcgttctacg tactccggtt
     1681 cgaggaacgc atcgggattg aggaggagca cgaattcgcc gttggcgagg cgtacgccct
     1741 gattgaccgc cgcgctgaac ccgcggttcg tatcgttggt gatctgccgt gcgaatggga
     1801 aagcactccg caccatggcg atcgaatcgt cgccggaggc gttatcgatg tgaagtagct
     1861 cgatgctgcg gtgagtctgt tgcgcgagag cggacaggca gcgttccaga aatcgcgcgc
     1921 tgttccagct aacgatgatg acggagacca atccgttcgt cagaccccgc tccatcgtgc
     1981 gaacgccttc cgcatcacgc cggcataggc gcgtgcggcg tgcagacgcc cgaggtgacg
     2041 gttggcgaga ccggcgagcg cgagggggat gcgcaggagc atcccgaaaa cgatcgccca
     2101 gcgcaggact tcggcctcac ctggcgtgtg ccatttgcgc gcatagagcc acatgttgcg
     2161 gtaccagagc gcgatgaact ccgcgaacgg aacgtgctcg agactggcgc ccccgaagtg
     2221 ccgcgcgcgg gctgcaggta cgacaaagat cgttttgccg gccgctgcga gacgccggca
     2281 gtagtcgacg tcttcgaacc aggccggcgt gaaccgctcg tcgagcgggc cgacttctgt
     2341 gaagacttcg cgccgcacca gcaatgccgc cgcggccggc tgctcgaccg gctgcggatg
     2401 cgtcagctcg agctcgcggt agcggtagcg cgccgtcatc gggttgtcgg ggaaaagctt
     2461 gtcgatgccg aaggcttcac tgatgagcgt ggccagcgtc ggcagccgcc gcaactgaaa
     2521 ctcgcgctgc gattcgcccg cttcatcgga aagaagaggg gcggcagcgg caatggccgg
     2581 attctcgtcg agaaacccca ccaggatctc gagcgagcgc ggcgagagct cgcagtcggg
     2641 attgaggagg aagagatagc gcccgctggt gcggctggcc acgcgattga cgccggccgc
     2701 aaagccgtcg ttgacggcgc cgcgaatcac ttcgacatcc ttccccatcg ccgccagcgt
     2761 ggcatcagac gaggcgttgt cgtaaacgac cacctcatat tcgagccggc cgcaggcggc
     2821 ccggacagat tgcaggcagc gctcgatcca acgttcgctg ttccacgtca cgatggcgat
     2881 cgagagatcg agcggaccgg tggcaatgcc gcgcaacatc ggcgccggct ggtcgctgct
     2941 cagttttctc gttgcctttt ccattctttg actgcctcga tcagcggacc ggtcaccgca
     3001 gcccacccga atcgaggctg gatccggatc aggttagata cgcaccgcgc atagaaagcg
     3061 tcgtccgtca tcaatttcgc gatcgcatcg gcgacagccg gtgcgttcga tggtggcaca
     3121 acgataccca attcctccgc cgccaccagc tcagcagcaa acccttcctc agttgtgatg
     3181 accggaaccg ccgccgcaat cgcgtcgaac aacctcgttc gaatcgagta cgcagcttcc
     3241 gcagtcggac gatgaagcat gatagcgact ttgctgcggc ggagccacga aagacggctg
     3301 ttgtacggca tccacgagtc gttggcctcg acgaacgctc cgcctgcggt gatccactcg
     3361 tcgagacggt cttcgcgcct ccggtcgacg gtgtgctgat tcggacggga acggcccagg
     3421 aacagaaggc ggcagtcgat gccgcggcgg ttgaccgcga tagttgcctc aaccgcggtc
     3481 gcaggatcga gccactccca cgtcccgcca ccccacaaaa cgatgttctc gcgatcttgt
     3541 ggtgcggctg cgtcgaagtc ttcgacgccg aacgggacct cgatgatggg gccttgcgcg
     3601 ccgcgataga gctcccgctg tttcttcgtc gcgacgacga gcaggtcgcc ggtggcaacc
     3661 gctcgccgca ctcgccagcg ctccgcctcg acgtgaaccc tctggcgcat cgaaggatgg
     3721 cggccgtaca tctcgtgcag ttccagaagc acgggatcaa agaggtcgta aacgatccgc
     3781 tgtccgcggc gctgccgccg gaaaccgcgc gcaggctgcc cgatcagtac ctcggcctca
     3841 cgtattcgcg catttgcttc ggcactgtcg cgagcagcaa cgcggaaact ggccgcacca
     3901 ggaagctctc cttcgcgttt gcagaccagc gtgaccgtga agtgcttcgc cagctcgccg
     3961 gccagatgcc acgcacgaat ccccgggcca gccatccgtt cgccgacgac gtccggcgtg
     4021 tgcacgacga tgttcacggc gcgcactcta tccaaggatg aagtcggaag gacgaaggat
     4081 gaatggaaag cactcttctt tcatccttca tccttcatcc ttcgtgtcag cccttcaacg
     4141 attccttgca gcgacgagcg tcccttcatc agtaacttcg tgtcttccca cagaagcgca
     4201 ctaatgcggc cgacaccgcg atggcggcgt gccacgaagt agcggttgcg cgttcgaagg
     4261 cggagtgctt cgccgaggag gagaggtgcc gactgcgaac cgcgatgcgt tgccttgatc
     4321 accggcagca cctccgtgcg ccagccggct tcgtgcagac gcgcgcagag gtcgacgtct
     4381 tcgtagtagg caaagaactg ctcatcgaag atggtgcggc cgagagcctc gcggcggtaa
     4441 agcgcggctg tggcagagac gccccatagg ggggtggggg gtcgggagtc gggggtcgga
     4501 acgagcgact tggtcttggc tccgactccc gactcccggc tccccactcc cgcagcgatc
     4561 tgtcgaaacg ttccgtcgct gatgtcgatg ccggcgccgt cgatcgtggt ctcgtcgcgg
     4621 cggaggatcg tttgcacagc ggcgagtttc ggatcgttgt cgagcgcgga agcgacggta
     4681 gcgagccagt cgcgatcgag aacgacgtcg ttgttgacga gcgcgatgta gttgccggtt
     4741 gcgtgacggt aggcttcatt gttgccgccc gcgaagccga ggttggtctc gcttcggaag
     4801 aggtgaaggg cgtgccggaa ggcgatgcgc tccgcggccg gcgtgcgcga gccattatcg
     4861 aaaacgatca cttccttcgg cggcagggtc tgcgaggtaa tcgattcgag acaggcgcgg
     4921 agcatgtcgc caccgtcgat atcgaggacg atcacggaga cgtcagtcat gctgcatcca
     4981 gtcgagattg gcgcttccgt cgaggcagcg caactgtgcg cgcccgcccg gaccagtgtt
     5041 gatcgtcgtg tatccgtaga tcgcaccact cggcggcagg acgaaggcat gcccgccgat
     5101 ctcgatcgtg gccccgccgc gcgactgata acgaagcatg gaaggtccgg gcttggccag
     5161 gaacgagagc gtgtcgccag cgtgaacgac ccaaccgccc tggtagagaa aacgcgaaac
     5221 ggtccattcg gcagggaaaa gctcgccgcc gttgtggatc acgtgcgcgt cttcgaattc
     5281 gacgcggtca gcgggtctgc gtccgtaggt aaatgccgcg agcaagatcg cggcgaagag
     5341 cagcggtgcg atcccagtca tcgtgcgccg aggagagcgg gcgtggggag tggggagtgg
     5401 ggggtgggaa tcgtcggatg cgcggcggag aatgcgacgc agattcgatc cgcccggaaa
     5461 ctcccacacc ccactcccca ctccccactc ccttgatctt gcgataccaa ccataccgag
     5521 cactacgacc aatacgacgc tcgcaaccgt tgccgcaagg ttcgggcgga tgaagctcgg
     5581 catgagccgc gagaagtcgc tgtgccaccg ttcgctcagc gattctccga tggagttttc
     5641 accagtggcg agatgaaaga ggcgccaggg aaatgctgcg ccgtgcgcga cgagcatcag
     5701 cgtccagccg gcgatgagag ctcgcgtccc tgtacccacc ttctcccaca atgccgcgct
     5761 tccgagtgca aggaccggca tcaggacgac gatgtagcga agagggggag accagccccc
     5821 gtgccattcc gagcgcggca cgaggtaaag aatgtagagg agtgacgcgc tgatgcccag
     5881 acggaaacct tccggcatct cccgccagcg cgcaagcgca acgatcgcca gcacatagag
     5941 cggcgcctga aaaagcagac cgcctgcacc gtcgagcacc aggccgaaaa gaccgcgcca
     6001 atacgacatc ggtccgcccg gaatgagctc ctgccacgaa tgcgtgttca tcggattgcc
     6061 ggtgatcatc cacgccacga gaagaggaac agccaccacg gccccggcga tgacgacttg
     6121 cctgcgcgtg cgcaaggcgc gtgcgagcaa cacgatcgca atcagcccga atcggatttt
     6181 gagcagcacg agtgcgaaga tcgccgtgat ccatcgagcg ggacgccgtt gccgtacgcc
     6241 gcgcaacgcc tcgaggaagc agaacgccgc cggcacttcc ggccagatgc gcgtcgcgta
     6301 gaacacgatc ggcgggccaa aggcgatcag cgggaagagt gcgcgcgttg tcgcgtcgtc
     6361 gatgccttcg tcttcgagaa agcgaaccgt cgatcgcgca agaagcgctc cgaacaacgc
     6421 gatgacggcc agcgcacccg gcacacctcc gagcgcgtag cccggcgcca tcaggatcgg
     6481 caggaacggt tcatgccgcg agtactgctc gccatgcgct cccctcgggt ccccaggttg
     6541 cggagcgaga tcgggacggc cggtatcgga gtgcgcgatc tcccgatact gattggtgag
     6601 atcgagatcg tggtcgcgca ccagcgactc ggacatcatc aggtaataag gctcatcgcc
     6661 atcgatcggc gtaagcaatt gaaaggggat catcaccgcg tagccgattg cagcgatcag
     6721 gaaggcgcgg tttgcgctcc agcgcacgtc atcggcaaac gcgagaaagg cgagaggaat
     6781 cgcgaggatg atcacgccga gggttacgaa aacgagatag ggatcgacgt cgatgagcag
     6841 cacgcgccca taggcccaaa gaattgcgaa ggcgatcacc gccccaagct gcgtacccag
     6901 acagcgccgg agcacggggg cgcgcaatac caagagcggc attgcgagag cggcgacgat
     6961 gaaaagcgcc agtttcatcg ctttggatac tcgacggaat cgatcgccag ccgcgactgc
     7021 gcgtgaagat cgccagccgc aacgacgcgg agcgagacga tgtcggacgc gcaagtgacc
     7081 gtcgtgcggc ccgcgccgac gatccaggcg ttcgcgccgt attcgcggat ctcgcccggc
     7141 gaaaagcgcg gaagcgaatt gcgcgaggcg aaaaactgct cgcggcgcat gaagccgaag
     7201 tcggccgtgt caccgatgcg gatcgggcgt accgtcaccc gcccatgctg atcggtcgct
     7261 tcgatcgcgc ctaccacgtg tcccgatcgg aggcgttcgg tgtacccgcc gcacgtcgtc
     7321 acgatggctt cgcgaacgtg aggcggtacc gagacaggaa gcgactcgtt ggccgccaga
     7381 gcgtgagaga tcgtctgcac ctcccccacc gacggcgcgt agttgcgagc gatcggcaac
     7441 gcccgggcga tgatgccgct ccatggccag agcgcgaata tagccacgac cgccgcgtac
     7501 gacagcggcc gcacgcgatc gatcagagga gcgaggaaga cgatcgccgc ggccacatag
     7561 accgtcgcca gcgagtagcg cgcaaagcag gcgcagagga gcaggacggc ggcaatggcc
     7621 acgctcggga ggacgcggga actcgtgctc gccgagactc cgagactccg caacccaacc
     7681 acgagcaaag cgagtgcgaa tggcagaaac gtcaccatcc ccgcacgcgc cggcgtcgcc
     7741 acagcaacgg ccagcaccgc gatcaggaac gctggcgtcc cgccggctgg cccggcggcg
     7801 tctcgccgcc gcggcatcgc gaagagcaac gccaggcttc ctgcgagcac caccagctcg
     7861 cgaacgatgc gcacgtcgtg cagcggtatc cagcggagca gaacgattcc gacgacggtc
     7921 agcgcgattt ccggcggcga gacgccgccg agccagccgg cgggacgccg gcgttccatc
     7981 gcgctcgcgc tgctcgcgta gaccgccatc gtcgcggcgc cgtatgccag cgctacgatc
     8041 acgccatatg cgagcagccg cgtgcgctca tcggccagcg tcatcattgc cacgatcagg
     8101 agaggcaccg cgcattgcac cggcgccgcc cagcgcgtcc cacgcccggt ccacgcgcag
     8161 atcaagacca gcgcgatgac tagcgctacg atgagcggaa gatgggcgtc gatgggtgtc
     8221 gtcagcacac cacgttctcg gctggattgc gaacccactc cggatcgaaa ccttcgatcg
     8281 tgtcgggtgt catcgaggcg cggtagacga cgcgggcgtc gccacgaagc tggacctcgc
     8341 ttacgtcgct cccgtcgagg gcgaacgata cttcgagggt gattccactt cgggtcagga
     8401 ccttcacggg tgacgtcgtc ttcccgaaga gcgcgctcgt caccgccgag gcgacgacgc
     8461 ccgatccgca ggaaagagtc tctgcctcca cgccgcgctc gtaggtacgc acttcgatcg
     8521 ctcccggctc ccggacgacg acgaagttga tgttggcgcc ggcaggctgc agacccgggt
     8581 gcatacgaag ggcgcggccg agggggacga tgtcctgggc ccagaggttg tcgcgcagga
     8641 agacgacata gtgcggcacg ccgacgacga tcgagcttcc tcggatcacc gtctcgccga
     8701 cgcgcagcgg acgatccgct tggaacgaat gtggctgcgg cagggtaagc gtcacgagcc
     8761 cgccatcgct aacctctgca gccacgacac ctgcgcccgt ctcgatcgtc atcttcttcg
     8821 gcgcgatcac attcacgagt gcaaagcgcg ccgcacaacg ggtgccgttc gcgcagaagt
     8881 ctgcgggcga gccatcggcg ttgaggtaac gcatgcggaa cgtcgccttc cccgtgttct
     8941 cgatgaggat gacaccgtct gccccgaccg aaagatgggg agtacagatc cgcctggtga
     9001 gcgcggcggc atcgtcgatg cgccccgacc ggttatcgat gaccacgaag tcgttcccgc
     9061 ctccggccat cttcaagaac gagattggat cctgtctgga tgcttgcgtt tgcatgggtt
     9121 gcgcgtgaac ggcggacgcg agcgtgccgc cggaagtttt tgtgccgggc gtgggttccg
     9181 accggcggct cattctcgca tgtaacggag cgcttccctg attcgtctaa acgtagggac
     9241 gcaccggtgc cggaagccgc cgttccatcg gcaatggccg atatctcgtt caatcggagc
     9301 tattcgtgaa tcgacttcgc ttcactcgtg tcacctcggc catcctcatc gcagccctca
     9361 ccaccggacc tctggcagca cagcagaaaa cgccgactga actgccgaag ctcgtcgaga
     9421 acatcgacgt ccacgtcatc ggcgtcgatg tcgtcgtcac ggacaagaag ggcaacccgg
     9481 tcaccggcct gacgaaggac gacttccgga tcttcgagaa cggcgaggag aagcagatct
     9541 cgaacttcta cgagatcgac ggaaaagtgg ccaaacctcc ggtcacgacc ggaactgcgc
     9601 cagcgaccac ggcggccgca ccggcgccag cgccgaagga agagatcagc gagcagttga
     9661 agcgccgcat catcatctac atcgacaacc tctccctcgc tccattcaac cgcaaccgcg
     9721 tcttcaagca gatgaaggac ttcgtcaagg acgtcatgcg gccgggcgac gaggccatgc
     9781 tggcgaccta caaccgaagc ctcaaggtgc gcgtgccctt cacgcgcgac cccgtccaga
     9841 ttcaacagat gctcgactcc atcgctggcg agtcaggttt tggcattgcg aacaaaagcg
     9901 aagccaaaga cgtgatgggg cgcatcaggg acgcaacgaa ttacgacgac gctcttgcgg
     9961 atgcgcgtac ctatgcgtct tcggtcgacc acgatctccg gcagagcgtg gagtcgatca
    10021 acggacttct gacgacactg gccggcgtgg aggggaagaa gattctcatc ctgacgaccg
    10081 agggcttcca gattcagcca gggcgcgaag tcttcgccat gatcgatgac gtggcgaagg
    10141 acaaagggtg gcagtccagc tcgacgctcg aggcgatgag ctacaacggt gcgcctctga
    10201 tcgagtcgat cgccaaaacg gccaacgcca acggcatcac gatctacgca attcacgcag
    10261 gcggcctcgc cgcgggaagc gaagggacgt cggctgacaa ccagcaggcg atctcctaca
    10321 acgtcacctc cgcggccgtc tcgaatacca ccgagtcgct ccagctcatg gccgagctca
    10381 ccggcggtct ggccacgaca cgcacgaaca actttgccgg cgcattcaag aacatcgggc
    10441 gtgatctcga ctcgtactac tccctcgggt accgcgccgg aacggagcgc gtcgaccggc
    10501 agcgcaccct cgaagtgcgc gtcaacaaca aagctctcca ggtccgcaac cgtcagacct
    10561 tcgtcgagaa atcgacgttc caggacatga gcgaccgcgt catcgccaac ctcctgtaca
    10621 gaacgaagtc gaacgatctc aatgtccgcg tcaaagtcaa cgcgccgatt ccggctgacg
    10681 atctcttcaa gatcccggtc gagatccaga ttccggtcga caacctcact ctcctgcaac
    10741 agggtgaagc ctacatgggc ggcttttcga tctacgtcgt cgtggggaac aaggacggcg
    10801 acatgtcgga cgtgtcgcgc aagtcacacc agatcacgat tccgctggcc gactttccga
    10861 agagcaaggg gcggtactac acgtacacgc tcgacctgat gtgcgagccg ggcctgaaca
    10921 agatctccat cggcgtagtc gacgacgtct cgaacatcag cggcttcgac cggcaacagg
    10981 tgatcgcgca ggacctgcgg taacgttcaa ctccacaatc ggtagaggtt tgcaccactt
    11041 cgcgatttcc gaggtagcct cggtgtgtga ggaacctgac gctcaagcac tggcggttga
    11101 tcaaggatcg cggggcggag gcggcccgcg aaatggcact cctgtggcta gtcttctcgg
    11161 cgctggacgc gctgcttagt ggccggctga ctttgccgta tctgacgatg aatttcttcg
    11221 ggtgtgcgat gttatggtta ctcggtatct atcttgaaat cacggcgaag gaggcccaat
    11281 gaatccgcaa ctgtacgcga ccctgccatt cgtcatgttc ggcggttttc tactagtagt
    11341 aggcttgtgg tcgattcata agatccggaa agaggaagcc tcgaagcgtg cggcgggctt
    11401 caccgcctcg ccaccggaat ctcgctcgca ctcgggtaaa tgacatgatc gacatcaatc
    11461 ccatgtacta cggcacgctc gcatttctcg cgttttcggc gtgtctcatc atcccgggat
    11521 tgtttctcgt tcacagcatt aagaaggagc gagccaaaaa acgtccgatc ggcttcgtta
    11581 ccgcggcacc ggaatctcgc tcacaatccg ctgaatgaac ctttgttccg gcgagccatc
    11641 ggcgcgcagc aggcgccggt ccttcatcac gatccggtgc gacaacaccg gtcccgccag
    11701 ccgcttcaca tcctcgggga tcacgaagtc gcggccatta accatcgcca gcgcctgaca
    11761 ggccttgaag tacgtcagcg cgccgcgcgt agagacgccg agggctgctt cggcgtgcgt
    11821 tcgcgtcgtc tcgacgatcg tcatcaggta atcgacaagc gattcgttga cgtgaatcga
    11881 cgcgacgtgc gcctgcagat cgcggacttc ctgctcttcg aggaccggct cgaggtgctc
    11941 cagcgcgtcc tgcgccccgc cggagcgaag gagtttcttc tcgtcggttg gattcggata
    12001 accgatcgtc agcttcatca ggaagcggtc gagctgcgac tcggggagcg gatacgtccc
    12061 aacgtgctcg atcggattct gcgtcgccag caccagaaac ggatcgggta gcgccaggcg
    12121 cttcttctcg atcgagatca tcccctcgct catcgcttcg agcagcgctg actgggactt
    12181 tggagttgcc cggttgatct catccgcgag caggatgttc gtgaacaccg gtccggagac
    12241 gaattcgaac tcctgcgcat cctggttgta gatcgtcacg ccgacgatgt ccgcgggcag
    12301 caggtcgctc gtgaactgaa tgcgctggaa cgacagcgac aacgaccgcg ccagtgcatg
    12361 cgcgagcatc gtcttgccaa caccgggcac atcctcgagg agaatgtgcc ctttcgagag
    12421 taacgcggcg atgcaaaact gcaccacttc gctcttgccg cggatcacac gctcgacatt
    12481 cgcctgcagc gcagcgattt tctcgaacgt cgggtggcgt gaaagcggct gcgtcgtcat
    12541 ttcaaagcgc ccgatgcaac agaggggacc ggcatgtcat ttcgacgccg ccccgcgctc
    12601 gcaggtcaca acttcttgta gtgcggtccg cctccgcctt ccggcggtac ccatgtgatg
    12661 acctgatagg gatccatgat gtcgcacgtc ttgcaatgga agcagttcgt gaagttgagg
    12721 aacggtacct ttccgcggcc atcagcagtc ggttgcggct cgtaaacctg cgccggacag
    12781 aagtgaaaac atggattccc gaactcctcg gtgcagcgcg tgatgcagac ttcggtgtcc
    12841 gcgatcaaca gatgcgccgg ctgattctct tcgtgaatga cgccgccgtt gtacacgtcg
    12901 gtgac
//
LOCUS       DATLVW010000513         3651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_231648, whole genome shotgun sequence.
ACCESSION   DATLVW010000513 DATLVW010000000
VERSION     DATLVW010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231648"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..811)
                     /locus_tag="VNN25_15415"
     CDS             complement(38..811)
                     /locus_tag="VNN25_15415"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXH92971.1"
                     /translation="MKVLTRIVPLAYRVLRVESWARTRAGEGLFLAAFFAYKRWIEDP
                     HAAFAVRHPEVFAGGHILDVGANVGYTACLFARQVSPPFRVHAFEPEELNFARLKRVI
                     AARHLGTVIAAVQTAIGDREGTAAFRQNPDHPGDHQVVDTADECAIRVPITTLDVYAA
                     TNHIDPVKFVKIDVQGHELAVSRGMSDLVERSPMLEISFEYSGPASNAVVDWYRARGF
                     VLSIMRHDGGLTPLTDAALAAALQSRAYCDIFATRRGNL"
     gene            complement(808..2121)
                     /locus_tag="VNN25_15420"
     CDS             complement(808..2121)
                     /locus_tag="VNN25_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH92972.1"
                     /translation="MEAWKRYFHDLLAAEISSTTRYYDAVVQYGRVEGDDGYLLSRIE
                     SGSVALSETPPDLTAGDRRAAVLLNGTLNHHLDVQGLLAKLKPHLARTSRVMVVAFNP
                     YLQWLYRAAVRLRFERGQVPTTFLTITDLDNLCRLSGFERIRVRPAGYVPWRLLGLGS
                     VVNRILPAIPLLKHLSLASIIILRPVIGDDVERPASVSVVIPARNERGNIEAALQRMP
                     DIGSDFEVIFVEGHSSDGTWEEILRVREKYSDRMNIIPLQQSGKGKSDAVRLGFEHAS
                     GDLLTILDADLTMPPELLTRFVGAYRSGVADFINGSRIVYPMEGGAMRFLNRVGNVFF
                     AKALSWVLGIRLGDSLCGTKLLRRDDYARLVAWRRDFGDFDPFGDFEMLFASSVLGLG
                     IVDVPIRYRARQYGSTQIRRFHHGLMLLKMTLVGFFRVKLGRIPK"
     gene            complement(2121..3452)
                     /locus_tag="VNN25_15425"
     CDS             complement(2121..3452)
                     /locus_tag="VNN25_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92973.1"
                     /translation="MRAHAAVAGALLALGIWLSSGTLAPYAASVARPTIVEPCHYLVS
                     VDHPHHIAPFFMLMGAPPEYWQWSVVLRRILYPIAVYPFVRVAGVLAGGLIANILFSV
                     AAQIAFAGLIARRFGRSSGIATLWLLATYPGITYWAGLPYSYAGIVPASLFCMALLLR
                     TDEATHLREVIAAATMMGVAFLAYDLLPFFLPAAVITLTVRRRYTWAALAALLAVLPT
                     LVVLATFRAMSLEVSNSNTSTYGAILSSYLHPSWSGEWAASLARLPVVLASNFLFSNF
                     IFLPLLFLAVVVIRRGKCLGAPELAVLTAALALFLFNNAAPPYYGWQMRGEWIARIYQ
                     PIFVVFLMAIGRALQVLPQSRALKACILAAVIGNAAIVFGPVTLTPFGPWAYLRFYQH
                     APTTFMLANLRLYGRRPLGICRTTHEGDDLPNPDTEFNRPVYTYRYEKKTP"
     gene            complement(3531..3647)
                     /gene="rrf"
                     /locus_tag="VNN25_15430"
     rRNA            complement(3531..3647)
                     /gene="rrf"
                     /locus_tag="VNN25_15430"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          821 a         1028 c         1179 g          623 t
ORIGIN      
        1 catggttcgg tgagcaactt cgtctcgatg cgaagcttca taggttcccg cgccgcgttg
       61 caaagatgtc gcagtacgcg cgcgattgca gcgcggcagc aagcgcggcg tcggtgaggg
      121 gtgtgagtcc tccgtcgtgg cgcatgatcg acaagacaaa tccccgggca cgataccagt
      181 ccacgaccgc attcgaggcc ggtcccgaat actcaaagga gatctccagc atcgggctcc
      241 gttcgacgag atcgctcatg ccgcggctga ctgccagctc gtgaccctgc acgtcgatct
      301 tcacgaactt caccggatcg atgtgattcg ttgcggcgta gacatcgaga gttgtgatcg
      361 gaacgcgaat ggcacattca tccgctgtgt cgacaacctg atgatcgccg ggatgatcag
      421 gattctgtcg aaaggcagcg gtgccttctc gatcgccgat cgcggtttgc accgccgcga
      481 ttacggtgcc gaggtggcgg gcggcaatga ctcgcttcaa gcgcgcgaaa ttcagctcct
      541 cgggctcgaa ggcgtgcact cgaaacggcg gcgagacctg acgcgcaaac aaacacgcgg
      601 tgtagccaac attcgcgccg acgtcgagga tatggccgcc tgcgaaaact tccggatgcc
      661 gtacagcgaa cgcagcatgc ggatcttcga tccatctctt gtaggcgaag aaggccgcga
      721 gaaacagccc ctcgccggcg cgcgtgcgtg cccatgattc gacgcgcagg acccggtagg
      781 ccagagggac aatacgcgtc agaaccttca tttcggaata cgcccaagct tgacgcgaaa
      841 aaacccgacg agggtcatct tcaacagcat gagcccgtgg tgaaagcggc gtatctgggt
      901 ggacccatac tgacgggcac ggtaacgaat cggtacatcc acgatgccga gtcccagcac
      961 cgaggaagcg aaaagcatct cgaaatcgcc gaagggatcg aagtcgccga aatcccttcg
     1021 ccacgccacc aggcgggcgt agtcgtcgcg gcgcagaagc ttggtgccgc aaagggaatc
     1081 accgaggcga atgccaagca cccagctcag cgccttggcg aagaagacgt tgcccactcg
     1141 gttgagaaac cgcatggccc cgccctccat cggatagacg atgcggctgc cgttgatgaa
     1201 gtcggcgacg ccgcttcgat acgcgccgac gaatcgggtg agaagctccg gcggcatggt
     1261 caggtcggcg tcaaggatcg tgagaaggtc tccgctcgcg tgctcgaaac caagccgcac
     1321 cgcgtccgat tttcctttcc cgctctgctg aagggggatg atgttcatgc gatcggagta
     1381 cttttcacgg acccgaagga tctcctccca ggttccgtcg gaagaatggc cctccacgaa
     1441 gatcacctcg aagtctgagc cgatgtcggg cattcgctgt agcgcggcct caatgttgcc
     1501 acgttcgttc cgagccggga tgacaacgga aaccgaggcc ggtcgttcaa cgtcgtcgcc
     1561 gatcacgggg cggagaatga tgatcgaagc cagggagagg tgtttgagaa ggggaatggc
     1621 cggaaggatt cggttgacga ctgaaccaag gccgaggaga cgccaaggca cgtagccagc
     1681 gggtcgaacg cgaatccgct cgaagccgga gagccggcag agattgtcca gatcggtgat
     1741 ggtgaggaag gtagtgggaa cctggcctcg ctcgaatctc aatcgtaccg cagctctgta
     1801 gagccattgc agataaggat tgaaagcgac gaccatcacg cgcgaggtgc gcgccagatg
     1861 cggcttgagc ttcgcgagca atccttgtac gtcgagatgg tggttgagcg tgccgttgag
     1921 aaggaccgcc gcgcgtctgt cgccggccgt aaggtcaggc ggcgtttccg aaagcgctac
     1981 gctcccgctc tctatccgcg atagaaggta gccatcgtcg ccttcgacgc gtccatattg
     2041 aacgaccgca tcgtagtagc gggtggtcga ggaaatctcg gccgcgagca ggtcgtgaaa
     2101 atatcgcttc caagcctcca ttacggtgtc ttcttttcgt agcgatacgt gtacaccggc
     2161 cggttgaact cggtgtcagg gttcgggaga tcgtcgcctt cgtgcgtggt gcggcaaatg
     2221 cccagagggc ggcgaccgta gaggcgaaga ttagcaagca tgaatgtggt aggggcgtgc
     2281 tggtagaacc ggaggtaggc ccatggacca aacggtgtca aggtgacagg gccgaatacg
     2341 atcgcggcgt ttccgatgac agccgccaga atgcacgctt tcagtgccct cgattggggc
     2401 agcacctgta gcgcacgtcc gatcgccatg agaaatacca cgaatatcgg ctgatagatt
     2461 cgcgcaatcc actccccgcg catttgccac ccgtagtaag gcggagctgc gttattgaag
     2521 agaaagagcg cgagcgcggc agtgagcacg gccagctcag gagctccgag gcacttgcct
     2581 cggcggatca cgacgacagc gagaaaaagg agcggcaaaa agatgaaatt gctgaacagg
     2641 aagttgctgg ccagaacgac cggcagacgc gccaggctcg cagcccattc cccgctccag
     2701 ctcggatgca agtagctgct caggattgcc ccgtaagtcg acgtgttgct attggagacc
     2761 tcgagcgaca tggcgcggaa cgtggccagc acgaccaacg tgggcaaaac ggctagtaac
     2821 gcggccagtg ccgcccaggt gtagcggcga cgcacggtga gggtgattac tgctgccgga
     2881 aggaagaagg gaagcagatc gtaggcaaga aaagcgacgc ccatcattgt ggcagcagcg
     2941 atcacctcgc gaaggtgggt cgcctcgtca gtacgcagga gaagggccat acagaatagt
     3001 gacgcaggca cgatccccgc gtaactgtag ggtaaccccg cccaatacgt gatgcctggg
     3061 tacgttgcca gaagccacag agtggcgatc ccgctggagc ggccgaatct gcgcgcaatc
     3121 aggcccgcaa aggcgatctg cgcggccacg ctgaagagga tattggcgat gagtcctccc
     3181 gcgagcacac cggccacccg gacgaacgga tagaccgcga tcgggtaaag gatgcggcgc
     3241 agaacgacgc tccattgcca atattcaggc ggcgcgccca tgagcatgaa aaagggggcg
     3301 atgtggtgag ggtgatcgac gctgacgagg tagtggcaag gctcgacgat ggtgggtctg
     3361 gcgacggagg cggcgtaggg agccagcgtt ccggaagaca gccagatgcc gagcgcgaga
     3421 agcgcgccgg cgaccgccgc atgtgcccgc atgggcgaga gaatacggga gcggtgcgga
     3481 aatgaaaagg aaaaggccct cgattgctcg agggcctttc ataaatttta tcccggcggc
     3541 gtcctacttt cccacacagt tgcccgcgca gtatcattgg cgatgagagg cttaactgcc
     3601 gtgttcggaa tgggaacggg tgtgaccctc tctctatgac caccggaaac t
//
LOCUS       DATLVW010000514         2413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_356530, whole genome shotgun sequence.
ACCESSION   DATLVW010000514 DATLVW010000000
VERSION     DATLVW010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356530"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..481
                     /locus_tag="VNN25_15435"
     CDS             110..481
                     /locus_tag="VNN25_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HXH92974.1"
                     /translation="MAKAKVKEHPYQEHIDALRAKQTQIHDSFQRDKVDGKAQADDGI
                     QDLADKAASAYSKELNFSLSDGERNLLMQIDEAFTRLKNETYGTCTNCGATIGEKRLA
                     AVPWTPYCIDCQELQEKGLLT"
     gene            complement(532..>2413)
                     /gene="ppdK"
                     /locus_tag="VNN25_15440"
     CDS             complement(532..>2413)
                     /gene="ppdK"
                     /locus_tag="VNN25_15440"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXH92975.1"
                     /translation="ISKAQAIGDEKSLEETMPSVYSQLEDVYKKLEAHYRDMQDIEFT
                     IQSGKLYLLQTRTGKRTGFAAVKIACDMVDEGLIDHTEAVARVEAGQIVQLLAPVFDA
                     KEKAKALSGGRLLGRGLPAGPGAAAGRIAFNAEHAVQMAKEGKGKGENGSDEDVILVR
                     IETSPEDIAGMHSAAGILTTRGGLTSHAAVVARGMGRPCVVGAGSIRVDYSKGELRSE
                     GHEPLEEGDWVSIDGSTGEILGGKLDTRPSEVIQVLLEESLPADKSETFRNFDRLLKW
                     ADELRTLGVRTNADTPTDARVARLFGAAGIGLCRTEHMFFSEDRILRVREMILARNEE
                     GRRKSLEQLLPFQKADFEGIFRELDGLPVTIRLLDPPLHEFLPHSEQQISAVARDMGW
                     GFVELRAKVNQLHESNPMLGHRGCRLAVTYPEIYEMQVRAIFQAACDMKKDGIDARPE
                     VMIPLVGTVPELAILKEMVQRVAAEVIKERGVELPVIVGTMIEIPRAALIADKLGEVA
                     EFFSFGTNDLTQMTFGYSRDDAGSFLPEYVDKKILPKDPFESIDQEGVGQLVRLGTER
                     GRSANPDLKVGVCGEHGGDPDSIRFFRSCGLNYVSCSPYRVPVARLAAAQAELEFKHF
                     EG"
BASE COUNT          465 a          782 c          705 g          461 t
ORIGIN      
        1 ccagatggcc cgtgccgttc ttctggaaca gatctacagg gcctgtacgt tgatgcgcaa
       61 cattccgtac cacaagtaag gagagccggt ctcgaacatg gcaaaagcga tggcaaaagc
      121 aaaagtcaaa gagcacccat accaggaaca catcgatgcg ttgcgcgcga agcagacgca
      181 aattcacgac tccttccaac gcgacaaggt tgacggcaaa gcgcaggccg acgatggcat
      241 tcaggatctt gccgacaagg ccgccagcgc ctactcaaaa gagctgaact tctcgctctc
      301 cgacggcgag cgcaacctcc tcatgcagat cgatgaggcg ttcacccgcc tcaagaacga
      361 gacctacggc acctgcacta actgcggcgc tacgatcggc gagaagcggc tggccgcggt
      421 tccctggacg ccctactgca tcgactgcca ggagcttcag gaaaagggcc ttctcaccta
      481 gaaagcaatg aaccacagag gcgccgagtg cgtctctgtg gtgttcacac ctcacccctc
      541 gaagtgcttg aactccagct ccgcctgggc cgccgctagg cgggccaccg ggacgcggta
      601 gggcgaacac gaaacgtagt tcaggccgca cgagcggaag aagcggatcg agtccggatc
      661 gccgccatgc tcgccgcaga cgccgacctt cagatcagga ttcgcgctcc ggcctcgttc
      721 ggtgccgaga cgtacgagtt ggccgacgcc ttcctgatcg atcgactcga acgggtcctt
      781 cggcagaatc tttttgtcga cgtactcggg gaggaacgag ccggcgtcat cgcgtgaata
      841 gccaaatgtc atctgcgtca gatcgttcgt tccgaatgag aagaactccg ctacttcgcc
      901 gagtttgtcg gcgatcaatg cggcccgggg gatctcgatc atcgttccga cgatcaccgg
      961 aagctcgacg ccgcgttcct tgatcacttc cgcagcaacg cgctgaacca tttccttgag
     1021 gatcgcgagc tcgggaaccg ttccgacgag cgggatcatg acctccggcc gcgcatcgat
     1081 gccgtccttc ttcatgtcgc aggccgcctg gaaaatcgcc cgcacctgca tctcgtaaat
     1141 ctcgggatac gtgaccgcga ggcggcagcc gcgatgaccc agcatcggat tcgattcgtg
     1201 gagctggttc actttcgcgc gcaactcgac aaacccccat cccatgtcgc gcgccaccgc
     1261 ggagatctgc tgttcgctat gtggcagaaa ctcgtgcagc ggcgggtcaa ggaggcggat
     1321 cgtcacgggc agtccgtcga gctcgcggaa gatcccttcg aagtcggcct tctgaaacgg
     1381 caacagttgc tcgagcgatt tgcgccgccc ttcttcgttg cgggcgagga tcatttcgcg
     1441 cacgcgcaga atgcggtcct cggagaagaa catgtgctcc gtgcggcaaa ggccgatacc
     1501 ggctgcaccg aagaggcgcg ccacgcgcgc gtcggttggt gtgtcggcat tcgtacggac
     1561 accgagcgtg cgcagttcgt ccgcccactt caacagccgg tcgaaattgc ggaacgtttc
     1621 ggatttatcg gcaggcagcg attcctccag cagcacttgg atgacctcgg aaggccgtgt
     1681 atccagcttg ccgccgagaa tctcgccggt ggatccgtcg atcgataccc aatcgccttc
     1741 ttcgagcggc tcgtgccctt cgctccgcag ctcacccttg ctgtagtcga cgcggatcga
     1801 gccggcgccg accacgcagg ggcgtcccat ccctcgtgcg accaccgctg catgcgaggt
     1861 gagtccgcca cgcgtcgtca gaatgcctgc ggcgctatgc ataccggcga tgtcttcggg
     1921 cgacgtttcg atgcgtacga gtatcacgtc ctcatctgac ccgttctcgc ccttcccctt
     1981 cccttccttt gccatttgga ccgcgtgctc ggcgttgaac gcgatgcggc ccgccgcggc
     2041 gcccgggccg gccggcagac cacgtcccaa cagccggccg cccgatagcg ccttcgcctt
     2101 ctctttcgcg tcgaagaccg gcgcgaggag ctgaacgatc tggcctgctt cgacgcgcgc
     2161 gaccgcctcg gtgtgatcga tcaaaccttc atcgaccatg tcgcaggcaa tcttgaccgc
     2221 ggcgaatcca gtgcgcttgc ctgtccgcgt ttgcagcaga tagagcttgc cggactgaat
     2281 cgtgaactcg atgtcctgca tgtcgcggta gtgggcctcg agcttcttgt agacgtcctc
     2341 gagctgcgag tagaccgagg gcatggtctc ttcgagcgac ttctcatcgc caatcgcctg
     2401 agcctttgag atc
//
LOCUS       DATLVW010000515         3603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_392548, whole genome shotgun sequence.
ACCESSION   DATLVW010000515 DATLVW010000000
VERSION     DATLVW010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392548"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VNN25_15445"
     CDS             <1..1507
                     /locus_tag="VNN25_15445"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXH92976.1"
                     /translation="PEDLARVLQDNAALINLGFIEGVTVNLTPLRRQAGIDVALDSAD
                     TRNQLRLHAVTDRAEGVGAVRSSTIATLTYSRRLFEATDVFGSYSKWRTGLLSRQADG
                     SAYQIGIRQRIDGMPDFLKRRGTIQGIVYLDPNMRGARIEGASPVAGVAVTLDGTRSV
                     RTDEHGHYAFRDVPPGIHHVAAEFSASKPAYFTTPSRVEVEPSATADFGLVWSSARVN
                     GNVISDASLGIAGVVVSAKAPDGKELTATTDSDGAFALSVPAGKYTIALAPATLPSGY
                     ISNGETAHEVDLKPDHPQQLAFEVRALRSISGTAAGTTEVQIEPLGRTALVDASGNFV
                     FRSLPTGTFTITAGAVTRTVTLPVEPATLSVTLDSPPAANGVSAAKSAAKSIPAADRV
                     PPTAPIILATLPPTLDVQPNLENRSEPRDHRFRVQAGAYRVAENVTIARRQLERLGYQ
                     ANVTTSGPLQLVSAGPFASRAEADRAAERLSKAGIDAIIVADRRDHGERRR"
     gene            complement(2098..2925)
                     /locus_tag="VNN25_15450"
     CDS             complement(2098..2925)
                     /locus_tag="VNN25_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92977.1"
                     /translation="MNTPCPTDETLALFASGDIDTSTRNNVLPHIETCRDCMAAILSA
                     NAYLEEERSARRGWTDTRWWMSAVAAAIVIAVISFPMIRSHNDPMSRLVALAPVSGRQ
                     VEPRLSGGFAWAPFHGPMRATDAAPDTDRLKLGGAAGDAIERAQRDHSQEAQRAAAVA
                     LLLIDRSTDAIDHLQAIASQAPSDARAWNDLAAARYAAATELRRPTLLPEALAAADRA
                     LAADPHSAEALFNRALILDRMSRTADARKAWLRYLEADSTSPWAAEARAHLRELPPG"
     gene            complement(2934..>3603)
                     /locus_tag="VNN25_15455"
     CDS             complement(2934..>3603)
                     /locus_tag="VNN25_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92978.1"
                     /translation="APLGAFLTVVVQRMLSREWMRVRGRWHPSAEAERSGDAAVLLEK
                     LTVRDGRSLDEAVAIVRNVDPSLDARSVRELSERLPQRPPRLRLVPLSDDDHGYVAPD
                     AADSRANAADARRVSARAARVVREMMATLPLQDRMLIRFHFGAELSIADAARLLGVPQ
                     RPLYRRIEALLRQLREALEREGVGPAAVEELISAGESESFDFGLRNGKDEPGRHTDDV
                     EAPN"
BASE COUNT          657 a         1247 c         1150 g          549 t
ORIGIN      
        1 gccggaggat ctggcccgcg ttcttcagga caacgccgct ctcatcaacc tcgggttcat
       61 cgagggcgtc acggtcaatc tcacacccct gcgccgccag gcagggatcg acgtcgcgct
      121 tgattcggcg gacacgcgca accagttgcg cctgcacgcc gtcaccgatc gcgccgaggg
      181 agtgggcgcg gtccggagct caacgatcgc gacgctgacc tactcgcgcc gcttgttcga
      241 ggcgacggac gttttcggct cgtactcgaa atggcgaacc ggcctgctct cgcgccaggc
      301 cgacggctcc gcgtaccaga tcgggatccg ccaacgcatc gacggcatgc ccgacttcct
      361 caaacggaga gggacgattc aaggcatcgt ctacctcgac ccgaacatgc gcggcgcgcg
      421 gatcgaggga gcgtcaccgg ttgccggtgt cgcagtcaca ctcgacggaa cgcgctccgt
      481 gcgaaccgac gagcacggcc actacgcatt ccgcgacgtt ccgcccggca tccatcacgt
      541 cgcagccgag ttctcggcgt cgaagccggc gtacttcacg actccatcgc gcgttgaggt
      601 agaaccgtca gcgaccgccg acttcggtct ggtctggtcc tccgctcgcg tcaacggcaa
      661 cgtcatcagc gacgccagcc tcggcatcgc cggagtggtc gtctcagcga aagcgccgga
      721 cggcaaagaa ctgaccgcga caaccgactc cgacggagcg ttcgcgctct ccgttccggc
      781 cggcaaatat acgatcgcgc tggcgccggc cacgctgccg tccggctaca tcagcaacgg
      841 agaaacagcg catgaggtcg atctgaagcc cgatcacccg cagcagctgg ccttcgaggt
      901 gcgcgccctc cgcagcatca gcggaaccgc cgccggcacc acagaagtac agatcgagcc
      961 cctcggccgc accgccctgg tcgatgcctc aggcaacttc gttttccgct cgctaccaac
     1021 cggaacgttc acgatcaccg ccggcgcggt cacgcgcacc gtcacgctac cagtagaacc
     1081 ggcaacgttg tccgtgacgt tggatagccc gccggccgcc aacggagttt ccgcagcaaa
     1141 gtccgcagca aagagcatcc ccgcagccga cagagtgccg ccaacagcgc cgatcatcct
     1201 cgccacgctg ccgccaacac tcgacgtcca gccgaatctt gagaaccgct cggaaccccg
     1261 cgatcatcga ttccgcgtcc aggccggcgc ctaccgcgtc gccgagaacg tgaccatcgc
     1321 ccgccgccaa ctggaacgcc tcggatatca agcgaacgtg acaacatccg gacccctcca
     1381 actcgtatcc gccggcccct tcgcatcccg agccgaagcc gatcgcgccg ccgagcgcct
     1441 gagcaaagcg ggcatcgacg cgatcatcgt cgccgaccgg cgcgatcacg gggaacgacg
     1501 acgctgatcg gattcgaact aataacccca gccgcgcatg gctgcagaac ttcacacggg
     1561 caagagcgtc gacgaatttc gacctccgcc cttacagcac tcgggcgagg acgcccgagt
     1621 gcccgcggtc gagacgaccg cactccgaaa agaggagtgt gggcgtctcg cccgcggcca
     1681 tcggggcgtc ccgcctcggt ggttgaatcc cgcctgatag ctcccaggga ttgaaacggg
     1741 tccagatctg aagagacccc taggtttcag gccgtgacgt cgagtcagcg caacgcctcg
     1801 ggccattttg aatgcggagg gtaggcgatc aagcatcccc caaggtctga tactgcttct
     1861 tcgcaacgcg gccagcgcaa ccggttacac ggctcgtgta gacgggaaca cagctcgtgt
     1921 ccatgtcaac acgactcgtg tacgcgtcaa caccgctcgc ggctacagtg cggggtcggc
     1981 ggcgagagcg ctcgcatttc gtgcaagtga gagcggtcaa aggatttagg ccgcaacccg
     2041 acagcactcg ggcgaggacg cccgagtgcc cgcggtcgag acgaccgcac tccgcggcta
     2101 acctggaggc agctcgcgaa ggtgtgcgcg tgcttccgcg gcccaagggg acgtggagtc
     2161 ggcttcgagg tagcggagcc acgctttgcg ggcgtcggcg gtgcggctca tccggtcgag
     2221 aatcagcgcc cgattgaaga gcgcctcggc ggagtgcggg tcagcggcca gggctcgatc
     2281 cgcggcggcg agcgcctcgg gaagcagcgt cggacggcgg agctccgtgg ctgccgcgta
     2341 tcgtgcggcc gcgaggtcgt tccatgcgcg cgcgtcagac ggcgcctggc tggcgatcgc
     2401 ctgcaggtga tcgatggcat cggtggaacg gtcaatcagc agcaacgcta ccgcggcggc
     2461 gcgctgcgct tcctgtgaat ggtcgcgctg ggcgcgctcg attgcgtcac cggcggcgcc
     2521 gccgagcttg agccggtcgg tatcgggggc ggcgtccgtc gcgcgcatcg gtccgtgaaa
     2581 cggagcccac gcgaaaccgc cggaaaggcg cggctcaacc tgacgccccg acaccggcgc
     2641 gagagcgacc agtctggaca tcggatcatt gtggctgcga atcataggga aggagatgac
     2701 ggcgatgacg atggccgcag cgacggcgct catccaccag cgcgtatcgg tccagccgcg
     2761 ccgggcgctc cgctcctctt cgagatacgc attcgcggaa aggatcgccg ccatgcagtc
     2821 acgacaggtt tcgatatgcg ggagcacgtt gtttcgcgtc gaggtgtcga tgtcgccgct
     2881 ggcaaacagc gccagggtct catcggttgg gcacggggtg ttcattgcac tgatcaattt
     2941 ggcgcctcca catcatcagt gtgacggcct ggctcatctt tcccgtttcg caggccaaaa
     3001 tcgaaagatt cggattctcc ggcgctgatc agctcctcga cggcagccgg cccgacgcct
     3061 tcccgctcca gggcctcgcg gagctggcgc agcagcgctt cgatccgtcg atacagaggg
     3121 cgctggggca cgccgaggag gcgggcggcg tcggcgatgc tgagctccgc gccaaaatgg
     3181 aaacggatca gcatccgatc ctggagcgga agcgtggcca tcatctcccg cacgacgcgc
     3241 gccgcgcgcg cggagacgcg ccgcgcgtca gctgcattcg cccgtgagtc ggcggcatcc
     3301 ggcgcaacgt atccatggtc atcgtctgac agcgggacga gccgcaaacg cggcgggcgt
     3361 tgggggaggc gctcagacag ctcacggacg cttctcgcat ccagcgacgg atcgacattg
     3421 cggacgatgg cgaccgcctc gtcgagagag cgtccgtcgc gcacggtcag tttctcgagc
     3481 agcaccgccg cgtcaccgct tctctccgct tccgccgacg gatgccatcg ccctcggacc
     3541 cgcatccact cgcgcgacag catccgctgc acgaccaccg tgagaaacgc gcccagcggc
     3601 gcc
//
LOCUS       DATLVW010000516         6078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_312402, whole genome shotgun sequence.
ACCESSION   DATLVW010000516 DATLVW010000000
VERSION     DATLVW010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312402"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VNN25_15460"
     CDS             <1..342
                     /locus_tag="VNN25_15460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HXH92979.1"
                     /translation="RIMADVTTTEINHSEHAPHVHVTPMWIYLTVFFALAVGTVLTVA
                     ASRYDLGMWNTPIALIIATVKAVLVILFFMHVIHSPRLTWVVVIASFLWLGVLFVLTF
                     ADYLTRTWNIY"
     gene            343..498
                     /locus_tag="VNN25_15465"
     CDS             343..498
                     /locus_tag="VNN25_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92980.1"
                     /translation="MLYILAVATLVIIYLCYTWMQSTRASQRRTEAQNQRVVELLEEI
                     LAELRSQ"
     gene            complement(582..1970)
                     /locus_tag="VNN25_15470"
     CDS             complement(582..1970)
                     /locus_tag="VNN25_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXH92981.1"
                     /translation="MLHFSLFNPHSARFMSAFRTELRQLLKLAGPLAAAQAGTQIMSL
                     VDLAVVGRLGARELGAAGLGNVIFFTISIVGMGLVFGIDPMISHALGGENPVRARHVF
                     WQGIWLALGVTVVLTIPLLAAPALFPLFGVELDLQPMATQFLLVRTAGLAPMLLFLVM
                     RAYLQAQHVTRPMVMAMLIGNVFNLVADFILVFGWGPFPRRGVPGAALSTDLGSIIAL
                     GILVWAVRKIELPPHDAGYLHHLKWPEIRQAIRVGLPVGLQMGAELGVFAFVGLLAGH
                     FGAIDLAAHQLVLAFASLTFTVAVGVASAGSVRVGLAVGARDQPRTRIAGFAALLGGF
                     CWMSIGALVFALAPRAVARLLTDQQTVITAAVPILLIAAVFQLSDGLQAIGAGILRGA
                     GDTTFPLIANLVSHWLIGLPIALYLGFHRNMGIVGLWWGLCVGLTVVAILLVARFNRL
                     SKKEIVPITRAA"
     gene            2057..3082
                     /gene="rfbB"
                     /locus_tag="VNN25_15475"
     CDS             2057..3082
                     /gene="rfbB"
                     /locus_tag="VNN25_15475"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXH92982.1"
                     /translation="MRYLVTGGAGFIGSNFVRYLFQKYGDAAQVVNLDKLTYAGIREN
                     LAEYEGKANYKFIHGDIAKAADVAEAYKGVDGSGVDVVVNFAAETHVDRSLMEAGTFI
                     ETDVHGVLVLLEEARKQTGLKRFIQISTDEVYGSIDTGSFTERDPLNPRNPYSASKAG
                     GDRMAYAYSQTYGLPVIVTRASNNFGPWQYPEKLIPLFVTNAIDDLPLPLYGDGRNVR
                     DWLYVEDHCAAIDFLIEHGVNGETYNIGGGNERENREITQTILSLTNKPDSLIKRVAD
                     RQGHDRRYSIDMSKVESMGFRCQTDFDEALRRTIQWYVENEPWWRAIKERSAEFKAYY
                     EKQYAAR"
     gene            4064..4777
                     /locus_tag="VNN25_15480"
     CDS             4064..4777
                     /locus_tag="VNN25_15480"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92983.1"
                     /translation="MDRQHRKDLKHDKFVDEIGSLSSRARENQRLLYMIAGGAVVLAV
                     LIYGIFFYRSNREQKAQQDLSTAIDTIESPLMPAAASQPVPNAKFKTEADRTAAAEKQ
                     FRAVTSNYSGTDASEVASLYLARIQAGKGDVAGARTLLQEFVNRNPKHLLVNGARYSL
                     YQIRIDNGEAPQVAQELQAEISKSEPSLPADSLLSLLAHDFEVQGNPEKTKETYRRIV
                     TEFPDSPYALEAQRKIGPA"
     gene            5166..>6078
                     /locus_tag="VNN25_15485"
     CDS             5166..>6078
                     /locus_tag="VNN25_15485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH92984.1"
                     /translation="MRLLTLIVLAAWVLAFFRTIVNLALIPRLRRDERPAREPLVSII
                     IPARDEARVIERTVRAFLAQTYPHFELIVVNDRSADATGAILRSIDDERLVVIDGEEP
                     SAGWLGKPWALHQGSRRANGEILLFVDADVIYAPDTLRAAVARFEQRQPALLTLLPFF
                     EMHGFGENAAMPMLALTCFTFLPTWLSNRTRGVALAIGGGTGNLVLRDAYVAAGGHEA
                     LKDSVVDDVGLARLIRHSGGLTTVVRADDLVSLRMYHGLREVIEGFTKNAFAVFGRNY
                     VVALLVAAGCVVFHILPFALALTGDRIS"
BASE COUNT         1219 a         1926 c         1878 g         1055 t
ORIGIN      
        1 cgcatcatgg ctgacgtgac cacgaccgaa atcaaccact ccgagcacgc gccgcacgtg
       61 cacgtcacgc cgatgtggat ctacctgacc gtgttcttcg cgctcgcggt cggaacggtc
      121 ctcaccgtcg ccgcctcccg ctacgacctc ggcatgtgga acaccccgat cgcgctgatc
      181 atcgccacgg tcaaagccgt cctggtcatc ctcttcttca tgcacgtcat ccactccccg
      241 cgcctgacct gggtcgtcgt catcgcctcg ttcctttggc tcggcgtcct gttcgtgctg
      301 acgttcgccg attacctcac gcggacgtgg aacatctatt aagtgctcta tatcctcgcc
      361 gtcgccacgc tcgtcatcat ctacctctgc tacacgtgga tgcagtcgac ccgcgcatcg
      421 cagcgaagga cggaggcgca gaaccagagg gtggtggagt tgctcgagga aatcttggcc
      481 gagctgcggt ctcagtaacg gagacggtca cgcgaagacg cgaagacgcg aagaagaaaa
      541 aatcacctct tcgcgccttc gcgtcttcgc gtgagcgctg gctacgccgc ccgtgtgatc
      601 ggcacaatct ctttcttgga cagccggttg aaacgcgcga ccaggaggat cgccaccacc
      661 gtcaacccaa cgcacaggcc ccaccacaat cccacgatcc ccatgttgcg gtggaagccg
      721 aggtagaggg cgatggggag gccgatgagc cagtggctga ccaggttggc gatgagcggg
      781 aaggtggtgt cgccggcgcc gcggaggatg ccggcgccga tggcttgcaa tccatcggag
      841 agctggaaga cggcggcgat cagcaggatc ggaacggcgg cggtgatgac ggtctgctgg
      901 tcggtgagca ggcgagcgac ggcgcgtggc gcaagagcaa agactaaggc gccgatgctc
      961 atccagcaaa agccgccgag gagcgcggca aagccggcta tgcgcgtgcg cggctgatcg
     1021 cgtgcaccga ccgccaggcc gacacgcacc gagcctgccg aggcgacgcc gacggcgacg
     1081 gtgaacgtga gcgaggcgaa tgcgagaacg agttgatgcg cggccagatc gatcgcgccg
     1141 aagtggccgg caagcaggcc cacgaacgcg aaaacgccga gctccgctcc catctgcagg
     1201 ccgacaggca acccaacgcg aatggcctga cggatctccg gccatttcag gtggtgcagg
     1261 tagcccgcgt cgtgcggcgg cagttcgatc ttcctgactg cccacacgag gatgccgagg
     1321 gcgatgatcg agccgaggtc ggttgacaga gcagcgccgg gcacgcccct gcgtggaaag
     1381 ggtccccaac cgaatacgag gatgaaatcg gccacgagat tgaagacatt gccgatgagc
     1441 atcgccatca ccatcgggcg cgtgacgtgc tgcgcttgca ggtaggcgcg catgacgagg
     1501 aaaaggagca tcggcgccag gccggcggtt cggacgagca ggaactgcgt agccatcggc
     1561 tgcaggtcca gctcgacacc gaacagcgga aagagcgccg gcgcggcaag gagggggatc
     1621 gtcagaacga ccgtgacgcc gagcgccagc catatgcctt gccagaagac gtgccgcgcc
     1681 cgtaccgggt tttcgccgcc gagggcgtgc gagatcatcg gatcgatgcc gaagacgagc
     1741 cccatgccga cgatggagat cgtgaagaag atgacattgc cgagtcctgc cgcgcccagc
     1801 tcgcgcgcgc cgaggcgtcc gacgacggcc aggtcgacga ggctcatgat ctgcgtgccc
     1861 gcctgcgctg cggcaagcgg cccagccagt ttcaggagct gacgcaattc ggttcggaac
     1921 gcggacatga agcgtgcaga gtgagggttg aagagtgaaa agtgaagcat gaagtaagct
     1981 tcatagccgc tcttcaccct tcacccttca ttcttcaccc caatcgtgct aaaaacgccc
     2041 gccttcagga gaccccatgc gttatctcgt taccggcggt gccggattca tcggatccaa
     2101 tttcgtccgt tacctttttc agaagtacgg tgacgcggcg caggtcgtca atctcgacaa
     2161 gctcacgtac gcgggcatcc gcgagaacct cgccgagtac gaggggaagg cgaactacaa
     2221 gttcattcat ggggacatcg ccaaagcggc ggatgtggcc gaggcgtaca aaggcgtcga
     2281 cggaagcggc gtcgatgtgg tcgtgaactt tgcggcggag acgcacgtcg accgctcgct
     2341 gatggaggcc ggcaccttca tcgagacaga cgttcacggt gtgctggtgc tgctcgaaga
     2401 agcgcgcaaa cagacgggcc tgaaacggtt cattcagatt tcgaccgatg aagtttacgg
     2461 atcgatcgat accggctcgt tcacggagag ggatccgctg aacccgcgca atccctattc
     2521 ggcgtcgaag gccgggggcg accggatggc gtatgcctac tcgcagacgt acggactgcc
     2581 ggtcatcgtc acgcgcgcct cgaacaattt cggaccgtgg cagtatccgg agaagcttat
     2641 cccgctcttc gtcacgaacg ccatcgatga tctgccgctg cctctttatg gcgatggtcg
     2701 caacgtgcgc gactggttgt acgtcgagga ccactgcgcg gcgatcgact ttctgatcga
     2761 gcacggagtg aacggcgaga cgtacaacat cggcggcggc aacgagcgcg agaaccgcga
     2821 gatcacgcag acgattctgt cgctgacgaa caagccggac tcgctcatca agcgcgtcgc
     2881 cgaccgacag ggccacgacc gccgctactc gatcgacatg agcaaggtcg agtcgatggg
     2941 cttccgctgc cagaccgatt tcgacgaagc gctccgccgc acgatccagt ggtacgtcga
     3001 gaacgagccg tggtggcgcg ccatcaagga gcgcagcgcg gagttcaagg cgtactacga
     3061 gaagcagtac gcggcgcggt agtgggctcg cgcggtctcg tgggctgagc tcttgctgcg
     3121 aaaccgcgac gccgcatctc tcacactgtt accaaatatt gatccctttt caatcgagcg
     3181 ccttctcccc cacgtctttt tgtgggggag aaggtggccg aaggccggat gagggggcgt
     3241 tgccacgagc gcgacatgtg tacatgtgcc gcgttttcag gcagttcgtg gttgttcgtt
     3301 ctgcgcatgg attcccccct catccggctt cgccaccttc tccccccgca aaaagcgcgg
     3361 ggggagaagg cgctcgatgg gagcactgcg aaggagaaag gacgcgcagc aacgctacag
     3421 gcccgtgcgg caacgacaca cccgccgtgc agcaacgcta cccctccaat gcagcaacgt
     3481 tatacgctcg cgccgcaacg ccacacgccc ggtgcagtaa tgcgacactg cccatgtggc
     3541 aacgctacac gtccagtgca gcaacgctac acccccggcg gagcaatgcg acacccccca
     3601 tgtagcagcg ctacaccccc ggtgtagcaa tgcgacacta cccatgtagc aacgctacac
     3661 ccccggtgta gcaatgcgac actccccagt agcaacgcca cacggccggt gtagcaatgg
     3721 gacgctgccc atgtagcaat gcaacaccca cacgtgcaac agcgttacat cgcgtatgcg
     3781 agctgtcacc cggaggtgac gtcatgacgc atcgacgtgt gggaagtggc ccccggctgg
     3841 aggtctctgc cacacgccga tttccggttg atgtttccag aggtgtgaat gcaacccacg
     3901 tcgaggaagt taaatgcccg caaaggcgtc tcgcgtcgca ggaaggttcc ggcacgcggc
     3961 cgcgagacaa cgccccaccc acacccggaa tccccaccct catttgaccg ttccccgacg
     4021 ctaggttaga gtcacccact ttttgacgga ggccgccctt tccatggatc gccagcatcg
     4081 caaagacctc aaacacgata agtttgtcga cgagatcggt tccttgtcgt cgcgggcgcg
     4141 ggaaaaccag cgcctcctct atatgatcgc cggtggcgcg gtcgtccttg ccgtcctcat
     4201 ctacggcatt ttcttctacc gctccaaccg cgagcagaag gcgcagcagg atctttcgac
     4261 agcgatcgac acgatcgagt cgccgctgat gccagccgcg gcaagccagc cggtccccaa
     4321 cgccaagttc aagacggaag ccgatcgcac ggccgcggcg gagaagcagt tccgcgccgt
     4381 tacctcgaat tacagcggca ccgacgcatc cgaggtggcg agtctttacc tggcccgtat
     4441 tcaggccggc aagggtgatg tggcgggagc gcgcacgctg ctgcaggagt tcgtcaaccg
     4501 caatccgaaa caccttctgg tcaacggtgc gcgctacagc ctttaccaga tccggatcga
     4561 caacggcgag gcgcctcagg ttgcccagga gctgcaggcc gagatcagca agagtgagcc
     4621 ttcgctgcct gccgactcgc tcctgtcgct tctcgcacac gactttgaag tccagggaaa
     4681 cccggagaag acgaaggaga cctaccgtcg catcgtgacg gagttccctg actcgccgta
     4741 cgcgttggaa gcgcagcgga agatcggacc ggcgtaacgg gattcgtccg atagtcaagt
     4801 ggcggagcag acaacggcaa atgttggatg ttgagtgttg aatgccggct aactaactga
     4861 ggacgtgtgt catcctgaga ctgccggagc cgccatacgt actcgagcct ccgctcagca
     4921 tgatgtggcg gcgtaggacg tcgaaggatc tcaaacgacg agacgcatgc catgtgccgc
     4981 gcatcttgag atccttcgcc gtcctgcggc gccgtcccat cacgcgtaac ggtcgcgagt
     5041 agtgcgggcg cctccggcag gctcaggatg acacccggga tgacacccgc cgttgggcgg
     5101 caaccaatat ccagcattca gcactcaaca tcatccaaca ttcaaattca acattcgcct
     5161 ttgacgtgcg cctgctaacc ctcatcgtcc tcgccgcctg ggtgctggcg ttcttccgca
     5221 ccatcgtgaa tctcgcgctc attccgcgat tgcgccgcga tgagcggccg gcgcgcgagc
     5281 cgctggtctc gatcatcatt cccgcccgcg acgaggcccg cgtcatcgag cggacggtcc
     5341 gggcgtttct ggcgcagacc tatccgcact tcgagctgat cgtcgtcaac gaccgctccg
     5401 ccgacgccac cggcgcgatt cttcgaagca tcgacgacga gcggcttgtc gtcatcgacg
     5461 gcgaagaacc ttccgccggc tggcttggca aaccgtgggc gctgcaccag gggagccgcc
     5521 gcgccaatgg cgagatcctc ctattcgtcg acgccgatgt gatctatgcc cctgacacgc
     5581 tgcgtgcggc cgtagcgagg ttcgagcaac gacagccggc gctcctgacg ctcttgccct
     5641 ttttcgagat gcacggattc ggcgagaacg cggcgatgcc gatgctggcc ttaacctgct
     5701 tcacgtttct tccgacgtgg ctctcgaacc gaacgcgcgg cgtggcgctg gcgatcggcg
     5761 gcggcaccgg aaatctcgtg ctgcgcgatg catacgtggc ggcgggcggc cacgaggcgt
     5821 tgaaagactc ggtcgtcgac gacgtgggac tggctcgctt gatccgccac tcgggcggtt
     5881 tgacgactgt agttcgcgcg gacgacctgg tctcgctgcg gatgtaccac ggactccgcg
     5941 aagtgatcga agggttcacc aagaatgctt ttgccgtctt cggccgaaac tacgtagttg
     6001 ccttgctcgt cgccgccggc tgtgtcgtct ttcacatcct gccgttcgcg cttgcgctga
     6061 cgggagaccg gatcagca
//
LOCUS       DATLVW010000517         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_148791, whole genome shotgun sequence.
ACCESSION   DATLVW010000517 DATLVW010000000
VERSION     DATLVW010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148791"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VNN25_15490"
     CDS             <1..199
                     /locus_tag="VNN25_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92985.1"
                     /translation="FIRRAVAFDRRAVAFVRRAVAFYRRAVAFIRHDVAFARVLSRLS
                     AGNRVYRPEIVFIGRAVAFFG"
     gene            624..>3452
                     /locus_tag="VNN25_15495"
     CDS             624..>3452
                     /locus_tag="VNN25_15495"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXH92986.1"
                     /translation="MSKHSRVYWQTCVFLLIALLIAAGASATTIVLPTDEQLVRKSPV
                     IVEGTVISSVPVLRGEGIWTETRLTVDRTLKGDAAGELTIREVGGEIDGRITKIFGSP
                     VYSPGERVMAFLAATPRGDYQTIDLFVGKFSEQRAANGRRLLVRDDAAGDVALLDASF
                     QPIRTKNVQRDATAFESYVADRVAGRASAASYGVADPVLERDLAPAAMNGTGRITADF
                     TLISEPSVYRWSAFDNGGSAKWYSFGTQPGYTGGGVNEIQTAMNAWNSYTAAKINYAY
                     SGTGSGTPGGLSGPNGVNEILFNDPLQEISGSFNPSTGGVVGEGGFNGVSGTMTWTGP
                     FDADGTHTAQTYRAYVISEGNLTIQDGVTPQAGIPSAMLAEIVAHEFGHTLGLGHSTD
                     NTALMYPSVTGLGASLRADDQLAARWLYPNGSAPPPPPVTVPSAPTSLVATTSGSTAT
                     LQWIDNATNESGYRVYFASGSGAFSRLSPDLAAGSRGATLSNLAIGSYRAYVVAFNSA
                     GESAASNTANITIATSTTAAFSVSPSSGVAGVTSFVFTNTSAGATSWLWNFGDGATSS
                     LQNPTHVYGSPGTYTIQLTATGSGGQSFASHAVSVTSGIAADFTFTPSNPTTQQNVAF
                     SDRSAGSVVSWQWNFGDGSSSTVQNPSKRYSFAGTYSVSLTVLSAAGLTSTITHAITI
                     VTATPAAPPVSAAFDATPSSPAVHDTVSFTDHSSGSPTSWQWSFGDGATSSAQNPTHA
                     YAAQGSYGVSLTVANAVSSSTISHTITIAAPTAYRTLVPVAAQTNGLGGSVWRTELTL
                     FNGGGEAAAGQFTFIPSGGGTLLTRSLFIGVNQSITFANALSDLFGLSAGAGAIAIEA
                     SSPTSTPAIRVTSRTFTTGSVGTYGQAVPNVSSDDLTTTLLLTGLESDASFRTNIGLV
                     NRSAAPVAAGMTLYDANGNVVATTVVTVP"
BASE COUNT          622 a         1150 c         1050 g          630 t
ORIGIN      
        1 gttcatccgc cgtgccgtcg cgtttgaccg gcgtgctgtc gcgttcgtcc gccgtgctgt
       61 cgcgttttac cggcgtgctg tcgcgttcat ccgccatgac gtcgcgtttg cccgcgtgct
      121 gtcgcgttta tcggccggaa atcgcgttta tcggccggaa atcgtgttca tcgggcgtgc
      181 tgtcgcgttt ttcggctgaa aatcacgttt ttcggctgaa aaaccgcgtg cacccgccgg
      241 attgtcgcga ttacccgtac gaccgcgcgg caacccgaag gccccgctcg taacttttgc
      301 acgttgctct gaattttcag ggtcggtccg atcgccggag cctgcttcag cgtgcgaaag
      361 aagagcagtc gcagcaagag tagcgggtgt cgagagtaag gggtagggtg ccggggaagc
      421 cagcgaactt tttcctctcc cacccccgac ccccgacccc cgggacaccc caccccgacc
      481 gcccatcccc cacccaggaa ttgccaacgc ccttcgcgta gttaaggccg aataactttc
      541 gtcccgtgat cctgaccgct ccccttccgg cttcgttccg gcgtacgcag ggagaggtct
      601 acccccatta ggagattgat cccatgtcga agcactcgag ggtgtactgg cagacgtgtg
      661 tcttcttact gatcgcgtta ttgatcgctg caggcgcctc ggccaccacg atcgtgttgc
      721 cgaccgatga gcaactcgtc cgcaagtcgc ccgtgatcgt cgaaggaacg gtcatcagct
      781 cggtacccgt tcttcgcggt gaaggaatct ggacggagac gcgccttacc gtcgaccgga
      841 cgttgaaagg cgacgccgca ggcgagctga cgatccgcga agttggcggc gagatcgatg
      901 gccggatcac gaagatcttc ggcagtccgg tctactcgcc tggcgaacgc gtcatggcct
      961 tccttgcagc gacgccacgt ggcgactacc agaccatcga tctctttgtc ggaaagttca
     1021 gcgagcagcg agccgcgaac ggccgccgcc ttctggtgcg cgacgatgcc gcgggcgatg
     1081 tcgcgctgct cgatgcgtcg ttccagccga tccggacgaa aaatgtccag cgcgacgcca
     1141 ccgcgttcga gtcctacgtc gccgatcgtg tcgccggacg cgcaagcgcg gcgagttacg
     1201 gcgttgccga tccggttctg gaacgcgatc tcgctccggc agcgatgaac gggaccggaa
     1261 gaatcacggc tgatttcacc ctcatctccg agccgagcgt ctaccgttgg agcgccttcg
     1321 acaacggagg cagcgccaag tggtacagct tcggaacgca gccgggctac acaggcggag
     1381 gcgtgaatga gatccagact gcgatgaacg cctggaacag ctacaccgcc gcgaagatca
     1441 attacgccta ttcaggcaca ggcagcggga cgcccggcgg actctccggc ccgaacggcg
     1501 tcaacgagat tctcttcaac gatcccctgc aggagatcag cggatcgttc aatccctcca
     1561 ccgggggcgt ggtcggcgaa ggtggcttca acggcgtctc cgggacgatg acctggacgg
     1621 ggccgttcga tgccgacggc acgcataccg cgcagaccta tcgcgcgtac gtcatcagcg
     1681 aaggtaatct gacgattcag gatggtgtca cgccgcaagc gggaatcccg agcgcgatgc
     1741 tcgcggaaat cgtggcgcat gagttcggcc acacgctcgg cctgggccac agcaccgaca
     1801 acaccgcgct catgtatccg agcgtcaccg gcctcggcgc aagtcttcgt gccgacgacc
     1861 agctcgccgc gcgctggctc tatccgaatg gcagcgcgcc accgccgcca ccggtcacgg
     1921 ttcctagtgc accaacgtcg ctggtagcaa cgacatccgg ctcgacggcc acgcttcagt
     1981 ggatcgacaa cgccaccaac gagagcggct accgcgtcta cttcgcgtcg ggcagcggcg
     2041 ccttcagccg gctgtcgccc gatctcgccg cagggagccg cggcgcgacg ttgtcgaatc
     2101 tcgctatcgg cagctaccgc gcctatgtcg tcgcgttcaa ttccgccggc gaatcggcgg
     2161 cttcgaatac cgccaacatt acaatcgcaa catcgactac ggctgccttc agcgtgtcgc
     2221 cctcgtcggg tgtcgccgga gtgacgtcgt tcgtcttcac caacacatcg gcaggcgcaa
     2281 cctcatggct gtggaacttc ggcgatggag cgacgtcgtc gttacagaat ccgacccacg
     2341 tctacggatc gcccgggacg tacacgatcc agctcacggc taccggaagc ggcggtcaat
     2401 cgttcgcgtc gcacgccgtg tcggtgacgt cgggaatcgc ggcagacttt acgttcacgc
     2461 cgtcgaatcc gacgacgcag cagaacgtcg ccttctccga ccggtcggcg ggcagcgtcg
     2521 tctcgtggca gtggaacttc ggcgatggct cgtcatcgac cgtgcagaat ccatccaaac
     2581 gctactcatt cgcgggcacc tactcggtgt cgctgacggt gttgagcgca gccggcctga
     2641 cgtcgacgat cacgcacgcc atcaccatcg ttacggccac tccggccgca ccaccggtct
     2701 cggcagcgtt cgatgcgacg ccgtcgtcac cagcagttca cgacacagtc tcattcaccg
     2761 atcactcgtc cggttcgcca acctcgtggc agtggagctt cggcgacggc gcgacatcgt
     2821 cggcgcagaa tccgacgcat gcgtacgcgg cgcagggttc gtatggcgtg tcgctgacag
     2881 tcgccaacgc cgtctcgtct tcgacgatct cgcacacgat cacgatcgcc gcgcccaccg
     2941 cgtaccggac gctcgtgccg gtcgcagcgc agacgaatgg attgggcggc tcggtttggc
     3001 ggacggaact gacgctcttc aacggaggag gtgaagcggc ggcgggccag ttcaccttca
     3061 ttccgagcgg cggcggaaca ctcctcacgc gcagcctttt catcggtgtg aatcaatcga
     3121 tcactttcgc caatgcgctg agcgatctct ttggcctctc cgccggcgcg ggagcgatcg
     3181 ccatcgaagc gtcgagcccg acatcgacgc cggccattcg cgtgaccagc cgaacgttca
     3241 cgaccggatc ggtaggcacg tacggtcagg ccgttccgaa cgtcagcagc gatgatctga
     3301 cgacgacact gctgctgacc ggcctcgaat cggacgcgag cttccggacg aacatcgggc
     3361 tcgtcaatcg cagcgccgcg ccggtcgcgg caggcatgac gctgtacgac gcgaatggca
     3421 acgtcgtcgc cactacggtg gtgaccgtcc cg
//
LOCUS       DATLVW010000518         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_148813, whole genome shotgun sequence.
ACCESSION   DATLVW010000518 DATLVW010000000
VERSION     DATLVW010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148813"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1119)
                     /gene="dprA"
                     /locus_tag="VNN25_15500"
     CDS             complement(52..1119)
                     /gene="dprA"
                     /locus_tag="VNN25_15500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXH92987.1"
                     /translation="MLDVSLRDRLIAYALLPLSPSRIRLLLQAFDPLASLCSASESML
                     RELLSIDAAAPLDPWRNDELRKRVDALRGSAVTLADDDYPPLLRQIVDPPLAMFFRGN
                     LSLARTPSVAMVGSRRASAYSISAATHLARELVSAGITVVSGLARGVDAASHQAALDA
                     SGHTIAVLGTGIDIVYPRSHRRLFEKIEERGLILTEFPPGTPPLKFNFPVRNRIISGL
                     TLGTVIVEATGRSGSLITARMAAEQNREVFAVPGPIFSPGSEGTHRLIQYGAKLVHET
                     GDILDELPGELRVPKASSPPLNTLLREVLRAFRRDEGTHIDNAAESLGRSVAAISEPI
                     LQLELGGWLKALPAGRYVRVR"
     gene            1244..1801
                     /locus_tag="VNN25_15505"
     CDS             1244..1801
                     /locus_tag="VNN25_15505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH92988.1"
                     /translation="MNRTLKGTLRLGTLATAMLLAAGCSHQSDLSRPASQDNFGVEMA
                     RMNLWREAMFRFERAIELNPGDAQAHNNLAVAYEANGDFEKARKEYLEALKLDRTNSY
                     IQKNYSRFVEFLSRNKKRQIAPKGTAAPATGSAPSAGSTTTSGQPDRPIGTQAPPPTG
                     LTPPAAPPSDRPQPPPAPKPPGGVA"
     gene            1798..2481
                     /locus_tag="VNN25_15510"
     CDS             1798..2481
                     /locus_tag="VNN25_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92989.1"
                     /translation="MKRVAAVIAFLLVPIASFAGVTEVKLKLPLKPKLSIGGGEKIAI
                     APFVIANREKKNDRASKVDVQAEFQRYLRKQLTKSTKLRLVDVPQTRLPGTTMKSLEA
                     SRDYWRNLGSQTGADFIVSGVIDFDVNDKSGYKTEEYVSPADGRTYYRQVLIETTGFV
                     FDIDIAVFNADTGEKVLEDNFRDFKEFDQRNYDEVLGLFENLRSMETQLVGIFVPQET
                     SATRYVFTE"
BASE COUNT          533 a          784 c          854 g          452 t
ORIGIN      
        1 cattgaacct tggctcgggt ttgctcatgc cggaattcta cctcggagac gctagcgcac
       61 gcggacatag cggcctgctg gcagcgcttt cagccaccct cccagttcga gctgcaggat
      121 tggctcggag atcgcggcga cggagcggcc tagcgactcg gccgcgttgt cgatgtgcgt
      181 tccctcgtcc cggcgaaacg cacgaagcac ctcgcgcaat agcgtgttca gtggcggcga
      241 tgaagctttc ggaacgcgga gctcgccggg cagttcgtcg aggatgtcgc cggtctcgtg
      301 aacgagcttc gctccgtact ggatcaggcg gtgcgtccct tccgagccgg gcgagaagat
      361 cggacctggg acggcgaaga cttcgcgatt ctgctcggct gccatcctcg ctgtgatcag
      421 cgagccgctc cgacccgtcg cttcgacgat cactgtgccg agggtgaggc cactgatgat
      481 tcggttccgg acggggaagt tgaatttgag cggtggcgtg cccggtggaa actcggtgag
      541 gatgaggccg cgctcctcga tcttctcgaa caggcgccgg tgtgagcgcg gatagacgat
      601 atcgatgccg gtgccgagga ccgcgattgt gtggcctgag gcatcgagcg cggcctgatg
      661 cgaggcggcg tcgacgcctc gcgcaagccc ggagacgacg gtgataccgg ccgagaccag
      721 ctcgcgagcg agatgcgtgg cggcgctgat cgagtaggcc gaggcgcgac gggagccgac
      781 catggcgacg gatggcgtcc gcgccagcga gaggttgccg cggaagaaca tcgccagcgg
      841 aggatcgacg atctgcctga gcaggggagg gtagtcgtcg tccgccagag tgaccgcgga
      901 accgcggagc gcatcgaccc gtttgcgaag ttcatcattc cgccacggat cgagaggcgc
      961 cgctgcgtcg atacttagca gctcacgcaa catggactcg gaggcgctgc acaacgatgc
     1021 gagcggatcg aaggcttgaa gcaggagacg aatgcgtgac ggtgatagcg ggagtagggc
     1081 atacgcgatc agacgatcgc gaagcgagac atcgagcatg ggcgtggttg gtgccgcagg
     1141 agggagcgat gcgtgggacg tcgccgggga caatggtaat cttgacagcc gtgtccggta
     1201 aagtccggat tgcatcgcct gccgctgtgg aaggagattc ccgatgaaca ggacgctaaa
     1261 agggacgctt cgactgggga cgctggctac cgccatgctg ctggcggccg gttgttcgca
     1321 tcagtcggat ctctcccggc cggcttcgca ggacaacttc ggcgtggaga tggcgcgcat
     1381 gaatctctgg cgggaagcga tgttccgctt cgagcgggcc atcgagctca atcccggcga
     1441 tgcgcaggcg cacaacaacc tcgccgttgc gtacgaagca aacggcgatt tcgagaaggc
     1501 gcgcaaggaa tatctcgagg cgctgaaact cgaccgcacg aattcctaca tccagaagaa
     1561 ctacagccgc ttcgtcgagt tcctctcgcg caacaagaag cggcagatcg cgccaaaggg
     1621 aacagccgcg ccggccacgg gctctgcccc atccgctggg tcgaccacga cttccggaca
     1681 gccggacagg ccgatcggca ctcaggcgcc gcccccaacg ggactgacac cgcccgcggc
     1741 gccgccgagt gatcgtccgc agccgccacc agcaccgaaa cctcccggag gtgttgcatg
     1801 aaacgagtcg ctgcggtaat cgccttcctg ctcgtgccga tcgcgtcgtt tgccggcgtg
     1861 acggaggtca aactcaagct gcctctcaag ccgaagctgt cgatcggtgg tggagagaag
     1921 atcgccatcg ccccgttcgt gatcgccaat cgggaaaaga agaacgaccg tgcttcgaag
     1981 gtcgacgttc aggcggagtt tcagcggtac ctgagaaagc agctcaccaa gagcacgaaa
     2041 ctgcgcctcg tcgacgtccc gcagacgcgc cttcccggta ccacgatgaa atcgctcgaa
     2101 gcgagccgcg attactggcg caacctcggc tcccagaccg gcgccgactt catcgtttcc
     2161 ggcgtcatcg acttcgacgt caacgacaag tcgggctaca agaccgaaga gtatgtctcg
     2221 cccgccgacg gcaggacgta ctaccgccag gttctcatcg agacgacggg gttcgtcttc
     2281 gatatcgata tcgccgtctt caatgcggat accggcgaga aagttctgga agacaatttc
     2341 cgcgacttca aggaattcga ccagcgcaat tacgacgaag tcctcggcct attcgagaat
     2401 ctccgctcca tggagacgca actcgtcggc attttcgtgc cccaggagac gtcagccaca
     2461 cggtatgtgt tcactgagta gggagtggga tttggggtgt ggggagtggg agaaagggat
     2521 cgcgacgcaa cgagatcgtc gtaactccca cacccaacac cccacagacc acacccattt
     2581 tggaggctaa tttgaagcga acgatcgctc tgctcttcgt cgt
//
LOCUS       DATLVW010000519         6801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_601061, whole genome shotgun sequence.
ACCESSION   DATLVW010000519 DATLVW010000000
VERSION     DATLVW010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601061"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1132
                     /locus_tag="VNN25_15515"
     CDS             50..1132
                     /locus_tag="VNN25_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92990.1"
                     /translation="MYAFPTREQPYSFTAARCAHLLGMCIDLDIGRSEEDAKDASQLL
                     NPIDALKGILRLIETGAIPLPTMTAQSGRGRYLIYIFASALPRGPEVRRRVRAVHADL
                     CKRLSGFSPDPSSGNTVQPFKVPGSPSVIGPVRYEMLEQGIITAPLYSLETMEEFLQV
                     EQEQSSSVDRQSTRSSRGGGIIRSSWQHVAPLRQRMEELCRLVPLRPRWKGYRHWLLL
                     AFAEAARRWFRAGRSIEDGERLAFDEILRFNRSLPDPEDEAVIRSTILRSRLLPRPQR
                     NVTIVRNLGITVEETMRAGLWSLAHLDTARRRREATQERQNKKRSRRCEIDDRLLSGE
                     GVLSIARSVGVSRATIYRRKRKLFRV"
     gene            complement(2026..2643)
                     /locus_tag="VNN25_15520"
     CDS             complement(2026..2643)
                     /locus_tag="VNN25_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92991.1"
                     /translation="MKKLIVAMVLAAAPIASAQDPFTHEDFDRVLSGAAWNQSPASFR
                     GIPFGGTESDLIAVLGPVKCADAGGVAVVRQCRATRKESRMMVDGVVIEDFYRFHAGK
                     LVEVLLERQRVMGEWGPAPSYASVLSVFSEKYGPPTETWKTRNKGVRGIVPFEAISAS
                     AKWQNDQISILIDGGDGSEFSYGIIQTGDWLRELAKMPRKNTTSF"
     gene            complement(2837..3085)
                     /locus_tag="VNN25_15525"
     CDS             complement(2837..3085)
                     /locus_tag="VNN25_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH92992.1"
                     /translation="MAASALALLGQTIREIRIERGMTQQQLADLCDRHRTFIIAIEKG
                     RQNASTMTLIRIASALRVLPSELFVKFTKSVMRGLPTD"
     gene            3160..3450
                     /locus_tag="VNN25_15530"
     CDS             3160..3450
                     /locus_tag="VNN25_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH92993.1"
                     /translation="METQARKKREPIAATMLARFLTANKLKPVEVAREAGVSRQHLCR
                     IRKGIADPGYMLAMRIRDACGRLLLRFVSIDELFDSSDPQALRMRAEVAKEK"
     gene            3450..3737
                     /locus_tag="VNN25_15535"
     CDS             3450..3737
                     /locus_tag="VNN25_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92994.1"
                     /translation="MSLPPQLLVVDVVTREHGETFADEWFRLSANVGTMTDACYKTGI
                     AEWPDEDEELQGIYVSIEREIVQRVADQLRNTVVETFIRLANEVLTRERRD"
     gene            3798..4028
                     /locus_tag="VNN25_15540"
     CDS             3798..4028
                     /locus_tag="VNN25_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH92995.1"
                     /translation="MSELNAIFFDDDGGITGFIEEFLGVFAHGKNIDEARSRLVEAAE
                     TFLEGSGPGVSQRRSAYGTVTRERMLVEFTLR"
     gene            4028..4360
                     /locus_tag="VNN25_15545"
     CDS             4028..4360
                     /locus_tag="VNN25_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH92996.1"
                     /translation="MDWRDRLRDAVNRSGKKHSYIAEDAGVTRATLSRILTGKHRNPG
                     VDTIVRIAHAVGESVGWIFGEQGFSLSGEQRAKIQTAAVILIDLTRGDPDRLTSLRAA
                     ASRSRRRR"
     gene            complement(4370..4561)
                     /locus_tag="VNN25_15550"
     CDS             complement(4370..4561)
                     /locus_tag="VNN25_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH92997.1"
                     /translation="MKVRELKDVLADAEDEAEVIVKKFRTGAVTGRRPDDVFEAFEAR
                     KQPSGKVSISYDESKRTDN"
     gene            complement(4669..4744)
                     /locus_tag="VNN25_15555"
     tRNA            complement(4669..4744)
                     /locus_tag="VNN25_15555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4709..4711),aa:Asn,seq:gtt)
     gene            complement(4793..5245)
                     /locus_tag="VNN25_15560"
     CDS             complement(4793..5245)
                     /locus_tag="VNN25_15560"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HXH92998.1"
                     /translation="MLTLYFAGAISGGRDDVDLYREIITALEADGHHVLAGAVASEHV
                     TASGESLDSRAIFDRDLGWIAECDILVAEVSKPSTGVGYEIATARYHRGIPVICLYRP
                     AHTTRCTAMVSGDRGIELIEYTNLPELLPRLSSALAKYTAVRPGLPPR"
     gene            5317..>6801
                     /locus_tag="VNN25_15565"
     CDS             5317..>6801
                     /locus_tag="VNN25_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXH92999.1"
                     /translation="MSREIVINAAKHESRIAVLDEGQVVELWVERTRHRTIVGNIYKG
                     RVTKVLPGMQSAFVDLGLERDAFLYVSDVLEDLEEFDNSDPTDDLALDDVPQQRAEAS
                     IADLLREGQEIVVQVSKDTIAGKGARITSHITLPGRFLVYMPTVNHIGVSRRIEDEGE
                     RARLKEILDRIRPPGAGGFIVRTAGEEREEEEFRADLKYLTDLWEQIRRKGEKASAPT
                     AIHHDLDLVLRTIRDVLSPEFKSVWIDSVDQYQRIVEFLDQIQPNLVSRVRLYRREEP
                     IFDDFSIEGEIAKALKSKVWLKSGGYIVINQTEALVAIDVNTGKYVGKKNLEETVFKT
                     NLEAAREIVRQIRLRDLGGIIVLDFIDMEDLANRTRLFEALEIEIRKDRSKTKILQIS
                     EFGLIEMTRKRVRQSLERSLTQACPYCGGSGRIKSNTTIAFEIWRELMKLRDLHEGQD
                     VIVRLNPVVYGSVHNTNDPIFEEIERNLGIHLVFKPDDSLHHEQF"
BASE COUNT         1451 a         1961 c         2076 g         1313 t
ORIGIN      
        1 ctcgggcgac gagtctgtct acacgacgct tgccgcgttc aatccttccg tgtacgcatt
       61 tccgacgcgc gagcagccct acagcttcac tgccgcgcgc tgcgcgcact tgctgggaat
      121 gtgcatcgac ctcgacatcg ggagatccga agaagacgcc aaagatgcat cccaactgct
      181 caaccccata gacgcgctga agggcatcct tcggctgatt gaaacaggag cgataccgct
      241 tcccacgatg acggcgcagt ccgggagagg gcgctacctg atctacattt tcgcatcagc
      301 tctcccgcgc gggccggaag tcaggcgtcg ggtgcgggcc gtccacgctg atctttgcaa
      361 acgcctatcc ggcttttcgc cagatccgtc gagcggaaac actgttcagc ccttcaaggt
      421 tccgggcagt ccctccgtca ttggaccggt tcgctacgag atgctggagc aaggaatcat
      481 tacggctccg ctctattcgc tcgaaacgat ggaggagttt ctgcaagttg agcaagaaca
      541 gagcagttcc gtcgataggc aatctacccg ctcttctcgc ggcggcggga tcatccgcag
      601 ctcctggcaa cacgttgctc ctttacgcca gcgcatggaa gagctgtgtc ggcttgttcc
      661 gctccgaccg cgatggaagg gatatcggca ttggttacta ctcgccttcg cggaagcagc
      721 gcgtcggtgg tttcgcgcgg gacgttcgat tgaagacggc gaacggctcg cattcgacga
      781 gatacttagg ttcaatcgaa gccttccaga cccagaggac gaggcagtca ttcggagcac
      841 gattcttagg tcaaggcttc tgccgcggcc acaaagaaac gtgacgatcg ttaggaacct
      901 cggcatcact gtcgaggaaa cgatgcgcgc cggactgtgg agccttgctc atctggatac
      961 cgcgaggagg cggcgggaag cgacgcaaga gcgtcaaaac aagaagcggt ctcggcgctg
     1021 cgagatagac gatcgtctgt tgtccgggga aggggttcta agcatcgcgc gcagtgtcgg
     1081 cgtttcgcgc gcaacgatct atcgccgaaa acgaaagctg ttccgggttt gatgtcgcat
     1141 tctttacctg tgaagtcccg gtgtctcact cttcacgagt gacggggggg aataccttca
     1201 caagtggaaa acgcgacagc ggcgaggggg tgctccacag cgagttttta atcaaaaaat
     1261 caagtgggtg taacccccga ttctgtaagc gcttagggcg cattttaccg tgttttcaga
     1321 tgtcaactgc gctcgggcag aaacggggga gaattggcag gcgtagactt gctggaaagt
     1381 cacccctttc ccgacccctc gttgcggcca cgagtggcac acttgtctac gcgcgcgcat
     1441 gtgcgacccg atttcgtgac attttgtgac atttggagag gcgctcatga ggctggtaag
     1501 ccctcaagac cgctagtaaa cttcggcccg atatggtggg gaaacaagca accgccgacg
     1561 cgaaagagcg ctttcttgcg gcactggaaa tgagccacgg caacgtgcgc accgctgcaa
     1621 acctcgcgaa cgtcggacgt cgctccgtct accgctggtt cgcgtcggat cagtcattcc
     1681 gccgcgagat ggcaatcaca tacctgatac gggctgatga aatccaggat gctgcgctag
     1741 atgcggcatc acggcggcgc ggcgataccg ggctgcttct gacacttgcc atcatgaacg
     1801 ctcgctattc atcaggcctg ctggatgagc agggggtgcg acgcctgggc atcaaggtaa
     1861 cggaggagga cttgaacaga gagaggaccg agagggagct tctcaactgc gagtcgccat
     1921 ccgttacgcg gctagctctc tcacgcctcc gaaagaggag tgccgtgctg ggggcgatcg
     1981 tcaaacctcc ccgacgctgg cgaccacgat aagaaggaag cgggtctaga aggatgtcgt
     2041 gttctttctc ggcatcttcg cgagttcgcg tagccagtcg ccggtttgga tgatgccgta
     2101 gctgaactcc gatccgtcgc cgccgtctat gaggatcgaa atctgatcgt tctgccactt
     2161 ggcagacgcg cttatggctt cgaatgggac aattccgcgg actcctttgt tccgagtctt
     2221 ccaagtttca gttggtggac cgtacttttc agagaatacg gacaagacgc tggcgtacga
     2281 aggggctgga ccccactcgc ccatgacgcg ctgtcgctct agcagaacct ccaccagctt
     2341 cccggcgtgg aatctgtaaa aatcttcgat cacgacgccg tcaaccatca tgcgtgactc
     2401 tttccgggtt gcgcggcatt ggcgcacaac ggcgacgcca ccagcatcgg cgcacttcac
     2461 cggaccgagg actgcaatga gatccgactc cgtcccgccg aatggtatgc cacggaacga
     2521 cgcggggctc tgattccaag ccgcgccgga caaaactcga tcgaagtctt cgtgcgtgaa
     2581 tggatcttgt gctgaagcaa tgggtgcggc agctaaaacc atagcaacga tcagcttttt
     2641 catggtggct ctccattctt cccgctgggc ttggaagagt agcgcatatc ggcatgcgaa
     2701 cttgaagttt ccagcacact caatcagcca cgaaaaggag caccagcaat ttgctcgggg
     2761 ttatcatctc gtgctcgcac gccaacgcgc atacgcccac ggcttgaaca gctcaccggg
     2821 cctcgcgagg ccgtgcctag tcagtcggca ggccgcgcat tactgatttc gtgaacttga
     2881 cgaacagctc cgatggaaga acgcggagcg ccgaggcgat gcgaatcaga gtcatggtcg
     2941 aggcgttctg tcggcccttt tcgatggcga tgatgaaggt gcggtgccgg tcgcacaggt
     3001 ccgccaactg ctgctgagtc atgccgcgct ctatccgaat ctcgcggatg gtctgaccga
     3061 gcaacgccag cgccgaggca gccatgacca cctgatccta ggagccgttt catgggaaaa
     3121 agtcgctttt aacttacgta gtcgataaaa tacattgcga tggaaaccca agcgcgaaag
     3181 aagcgggagc cgattgccgc gacgatgctg gcccggttcc tgaccgcgaa caaactcaaa
     3241 ccggtggaag tagcgcgtga ggccggagtg agcagacagc acctgtgccg gatcaggaag
     3301 ggcatcgctg atcccggcta catgctggca atgcgcatcc gggacgcctg cgggcggctc
     3361 ctgcttcgct tcgtcagtat tgatgaactc ttcgactcaa gcgatccgca ggcgctgcgg
     3421 atgcgtgcgg aagtcgcgaa ggagaagtga tgagcctacc gccgcaactg ctcgtcgtcg
     3481 atgtggtcac gcgcgagcat ggcgagacat tcgcggatga atggtttcgt ctgtcggcga
     3541 acgtcgggac catgactgac gcctgctaca aaaccggaat cgcggaatgg ccggacgagg
     3601 acgaagaact gcaagggatc tacgtcagca tcgaaagaga gatcgtgcag cgtgtcgcgg
     3661 atcaactacg aaacacggta gtggagacat tcatcagact agccaacgaa gtgctgaccc
     3721 gtgaacggag ggattgaatg gcaaccaaac ggaagcgaaa gaaaccgttg gatgaacttc
     3781 tgtacgggga gcgaaggatg tccgaactga acgcgatctt tttcgatgac gacggcggca
     3841 tcaccggctt catcgaagaa ttcctcggag tgttcgcgca cgggaagaac atcgacgaag
     3901 cgcgatcgag gctggtagag gcggcagaga cattccttga aggaagcggt ccgggcgtga
     3961 gtcagcggcg atcagcctac ggcacggtaa cgcgcgagcg gatgttggtc gagttcacgc
     4021 tgaggtaatg gactggcgcg accgtctccg cgatgcggtg aaccgctccg gcaagaaaca
     4081 cagctacatc gcggaggatg ccggggtcac gcgtgcgacg ctgagccgaa tcctcactgg
     4141 aaagcatcgt aatcccggcg tcgatacgat cgtgcgcatc gcccatgcgg tcggggagag
     4201 tgtcggctgg atattcggtg agcagggatt ttccctttcc ggcgagcaac gcgcgaagat
     4261 ccaaaccgcc gccgtgatcc tcatcgatct gacacgcggc gatcctgatc gcctcacttc
     4321 cctacgagcc gcagcgtcgc gttcaaggcg tcgcagatag attgcggcgt tagttgtcgg
     4381 tccgcttcga ttcgtcgtag gagatgctaa ccttccctga cggctgcttt ctcgcttcga
     4441 acgcctcgaa cacgtcgtcg gggcgtctcc ccgtcaccgc ccccgttctg aatttcttca
     4501 cgatcacctc ggcctcgtct tcagcgtcag ccagcacgtc tttgagttcc cgtaccttca
     4561 tctcacacac ctccatccga cgttctaggt cggcgcaatc tagtcacgaa tctagtcatg
     4621 acgcggtttt ggcgagtgac aagtgtgccg taaacgattg attctaaatg gctccggcag
     4681 taggactcga acctacgacc cacggattaa cagtccgttg ctctaccaac tgagctatgc
     4741 cggaacactt ggcgcggcgg gcggcggaag atagcaaagg gctgccacgc cgtcaacgtg
     4801 ggggcaaacc ggggcggacg gcggtgtatt tcgcgagcgc agatgagagg cgcggcagca
     4861 attcgggaag gttcgtgtac tcgatcaact cgatgccgcg gtcgcccgaa accatggccg
     4921 tgcacctcgt tgtgtgggcg ggtcggtaga ggcagatcac ggggatgccg cggtggtagc
     4981 gggccgtggc gatctcgtaa cccacgccgg tcgacggctt cgaaacctcg gcgacgagga
     5041 tgtcgcactc ggcgatccag ccgaggtcgc ggtcgaagat ggcgcgggag tcgagcgatt
     5101 cgcctgaggc agtgacgtgc tccgatgcga ccgcaccggc gagaacgtgg tggccatccg
     5161 cttcgagcgc ggtgatgatc tcgcggtaaa ggtcgacgtc gtcgcggccg ccgctgatcg
     5221 ctccggcgaa gtagagagtg agcatcgtgg agcgagtcta tcctgacgcg aagacgtcag
     5281 gacctcaact ccgcctcagg atagactctg aggcgaatgt cacgcgagat cgtcatcaac
     5341 gccgcgaaac acgagtcgcg catcgccgtg ctcgacgagg ggcaggttgt cgagctgtgg
     5401 gttgagcgta cgcggcatcg caccatcgtc ggcaacatct acaaaggccg tgtaaccaag
     5461 gtccttcccg gcatgcagtc ggcgtttgtc gacctcgggc tcgagcgcga tgcgtttctc
     5521 tacgtttccg acgtcctcga ggatctcgaa gagttcgaca acagcgatcc gacggacgat
     5581 ctcgcgctgg atgatgttcc acagcagcgc gccgaggctt cgatcgccga tcttttgcgc
     5641 gaagggcagg agatcgtcgt gcaggtgtcg aaggacacga tcgcaggcaa aggcgcgcgc
     5701 atcaccagcc acatcacgct accgggacgc tttctcgtct atatgccgac cgtcaaccac
     5761 atcggcgttt cgcggcgcat tgaggacgaa ggagagaggg cgcggctgaa ggagatcctc
     5821 gaccgcattc gtccgcccgg cgccggcggc ttcatcgtcc gcacggccgg cgaggaacgc
     5881 gaagaagagg agtttcgcgc ggacctcaag tacctcaccg acctgtggga gcagatccgg
     5941 cgtaagggtg aaaaagcatc ggcgccgacg gcgattcatc acgatctcga tctcgtcctt
     6001 cgcacaatcc gcgacgtgct ctcccctgag ttcaagagcg tctggatcga ctcagtcgac
     6061 caataccagc gcatcgtcga gtttctcgat cagatccagc ccaacctcgt ctcgcgcgtt
     6121 cgactttatc gccgcgagga gccgatcttt gacgacttca gcatcgaagg tgagatcgcc
     6181 aaggcgctca aatcgaaggt gtggctcaag tcgggcggct acatcgtcat caatcagacc
     6241 gaggcgctcg tcgccatcga cgtgaatacc gggaagtacg tcggcaagaa gaatctcgag
     6301 gagaccgtct tcaaaacgaa cctcgaggcc gcgcgagaga tcgtccggca gatccgtctc
     6361 cgcgacctcg gcgggatcat cgttctcgat ttcatcgaca tggaggacct ggccaatcga
     6421 acgcgtctct tcgaagcgct cgagatcgag atccgcaagg accgctcgaa gacgaagatc
     6481 ctgcagatct ccgagttcgg gttgatcgag atgacgcgca agcgggtgcg ccagagtctg
     6541 gagcgctcgc tgacgcaggc ctgtccgtac tgcggtggca gcggccggat caagtcgaac
     6601 acgacgatcg ccttcgagat ctggcgcgag ctgatgaaac tgcgcgatct gcacgaaggt
     6661 caggatgtga tcgtgcggct gaacccggtc gtatacgggt ccgttcacaa caccaacgat
     6721 ccgatcttcg aagagatcga gcggaaccta gggatccacc tggtcttcaa gccggatgac
     6781 tcactgcatc acgagcagtt c
//
LOCUS       DATLVW010000520         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_231994, whole genome shotgun sequence.
ACCESSION   DATLVW010000520 DATLVW010000000
VERSION     DATLVW010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231994"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..881)
                     /locus_tag="VNN25_15570"
     CDS             complement(105..881)
                     /locus_tag="VNN25_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXH93000.1"
                     /translation="MRIATLFLFLVIVALPLTADDAADFLKAVSGRDLATVKSMLARD
                     ASLANAHSAKGTDAVTLALFTNVGEGFTDPAKNEVLQALIPHAKLDIFEIAALGTAAQ
                     LDAMLTDPAALTSRTAFGWTLLHMAAFGGNAATTELLLRKGAALETRAKSKFLNTPLQ
                     TACLNGQYATAKILLDHGADVLVRQSKGFAPLHEAALLGRTDLVQLLLDHGAEINSVA
                     DNGQTPLAEAMRGKHDELAAWMKAKGAVLGVQPDDEVLKK"
     gene            1007..2080
                     /locus_tag="VNN25_15575"
     CDS             1007..2080
                     /locus_tag="VNN25_15575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXH93001.1"
                     /translation="MPHTRILGVAGLTAWLMVGLPVIIQGATLPGTMLRWAVAYVLFG
                     ALFVADLARPRRSLMAMEVLCIVVMVLLLCDGFEGTLLVLIAMRLPSLMDRKRGLAWI
                     VIQTLFLAGAVAFHWNGRSAWLLAPAYFGFQILAFFTFEILDREIRTNAQLRAMQDLV
                     ADGGRLAERLRIARELHDALGHHLTALTLNLEAALVRTDGAAHGDVEKAQALARTLLA
                     DVRSIVAEKNADDCLDLGRALRTLVADLPRPHVQLEVDQSLRVEDPEQGLVILRCVQE
                     VVTNAARHSGAENLWITVRRTAEGFEVRARDDGRGRASARDGFGLRGMRERIENAGGA
                     LTIESGTDRGFALTAFLPARSGL"
     gene            2077..>2407
                     /locus_tag="VNN25_15580"
     CDS             2077..>2407
                     /locus_tag="VNN25_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH93002.1"
                     /translation="MIRVFIADDQLLIRQGIRTLLEMDAEIVIAGEAEDGAETIERVP
                     AAEVDVLLLDVRMPKKSGVDVLRELSSANALPPTLVLTTFDDTPVVLDAIRAGARGFL
                     LKDISYQQ"
BASE COUNT          373 a          772 c          796 g          466 t
ORIGIN      
        1 cgcggctgga cgccggggtt cccctgcgat tgccgaagag tgcttaagtc aaggcttgga
       61 cacgatccgt gacgcgcgta cgtccttaat gctccgcttc acgcctactt cttcaacacc
      121 tcgtcatccg gttgcacgcc cagcacggcg cccttcgcct tcatccacgc cgccagctcg
      181 tcgtgttttc cgcgcatggc ctcggccagc ggagtctgac cgttgtcagc gacggagttg
      241 atctcggcgc cgtggtcgag caggagttgg acgagatcgg tgcggccgag gagtgcggct
      301 tcgtgcaatg gggcgaaacc cttcgattgg cggacgagca catcggcgcc gtgatcgagc
      361 aggatcttcg cggtcgcgta ctggccgttg agacacgccg tctgcaatgg cgtgttgaga
      421 aactttgact tggcgcgggt ttcgagcgct gcgcccttcc tcagcagcaa ctccgtcgtc
      481 gcggcattgc cgccgaaggc cgccatgtgc agcagcgtcc atccgaatgc cgtgcggctc
      541 gtcagcgccg caggatcggt gagcatcgca tcgagttgag cggccgtgcc gagcgccgca
      601 atctcgaaga tgtccaattt cgcgtgaggg atgagcgctt gcagaacctc gttcttcgcc
      661 ggatcggtga agccttcgcc gacgttcgta aacagcgcta aggtgacggc atccgttcct
      721 tttgcgctgt gcgcgtttgc gagcgaggcg tcgcgggcga gcatcgactt cactgtggca
      781 aggtcgcggc cggagaccgc tttcagaaaa tcggcggcat cgtctgcggt cagcggaagg
      841 gcgacgatga cgagaaacag aaagagtgta gcgattcgca taatgaaatc ctccgctgca
      901 caaggtacgc accgccacgc ttccgcgcat gtgccgctcg gggcaacgcg cggcatgtga
      961 cttccggcct attcacgggc ccgatccgct gtgtcatcct cccgccatgc cgcacacgcg
     1021 catcctcggc gtcgccggcc tgaccgcatg gttgatggtc ggccttccgg tgatcatcca
     1081 gggcgcaacc ctgcccggaa cgatgctgcg atgggcggtt gcgtacgtgc tcttcggcgc
     1141 gctcttcgtt gccgacctcg cgcggcctcg ccgttccttg atggcgatgg aagtgctctg
     1201 catcgtcgtg atggtgctgc ttctttgtga cggcttcgaa gggacgctgc tcgtgctgat
     1261 cgcgatgcgg cttccgtcgc tgatggaccg gaagcgcggc ctggcctgga tcgtcatcca
     1321 gacgttgttt ctcgccggcg cggttgcttt tcactggaat ggccggtcgg cctggctgct
     1381 cgctcctgcg tatttcggct ttcagattct cgccttcttc acgttcgaga ttctcgaccg
     1441 cgagattcgg acgaacgcgc agcttcgggc gatgcaggat ctcgtcgccg acggcggccg
     1501 cctcgcggaa cggcttcgca tcgcgcgcga actgcacgat gccctcggcc atcacctcac
     1561 cgcgctgacg ctgaacctcg aggccgcgct ggtgcgcacg gacggcgcgg cgcacggcga
     1621 cgtcgagaag gcgcaggctc tagcgcgcac gctgctggcc gacgtccgct cgatcgtggc
     1681 cgagaagaac gccgacgatt gcctcgacct cgggcgcgcg cttcgcacgc tcgtggccga
     1741 tctgccgcgg cctcacgtgc agctcgaggt cgatcaatcg ctgcgcgtcg aagatcccga
     1801 gcagggcctg gttattctcc gatgcgtgca ggaagtggtg acgaatgcgg cgcggcactc
     1861 cggcgccgag aatctctgga tcaccgtgcg ccgcactgcc gagggattcg aagtgcgtgc
     1921 gcgtgacgat ggccgcggac gggccagcgc gcgcgacggc ttcggcctgc gcggcatgcg
     1981 cgagcgaatc gagaacgccg gcggcgcgct cacgatcgag agcggcacag accgcggctt
     2041 cgcactgacc gcgtttcttc cggcgcggag tggactgtga tccgcgtctt catcgctgac
     2101 gatcagctcc tcatccgcca gggtatccgc acgctgttgg agatggacgc ggagattgtg
     2161 atcgccggcg aggccgaaga tggcgccgag acaattgagc gggttcctgc ggccgaggtg
     2221 gacgtgctgc tgctcgatgt gcggatgccg aagaaaagcg gcgtcgatgt tcttcgcgag
     2281 ctctcctcgg ccaacgcgct cccaccgacg ctggtgctga cgacgttcga cgacacgccg
     2341 gtcgtgctcg acgccattcg ggccggtgcg cgcggcttcc tgctcaagga catctcgtat
     2401 cagcaac
//
LOCUS       DATLVW010000521         2851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_475786, whole genome shotgun sequence.
ACCESSION   DATLVW010000521 DATLVW010000000
VERSION     DATLVW010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475786"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..658)
                     /locus_tag="VNN25_15585"
     CDS             complement(50..658)
                     /locus_tag="VNN25_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93003.1"
                     /translation="MKKEMIFKKVVAGIGSAALAIALSACAHHDAASQAEGYGQIRTE
                     GAATTADNSTPVVGTAPAPKNAEGVSGSTTPAVISGPAAVDSDGRAYTSSSAGGNGNA
                     SNLGNNTNVNLIPKKTSSSEVVVTQTPIESTTTTVVSNTPAPVEPMVVETPAPAPVVV
                     ETPAPAPAPAPVVETPMASSSTVETPAPEAPAPKHHRKMHKD"
     gene            766..2070
                     /gene="xseA"
                     /locus_tag="VNN25_15590"
     CDS             766..2070
                     /gene="xseA"
                     /locus_tag="VNN25_15590"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXH93004.1"
                     /translation="MADRVWQVSELIRQINLDLFRYNDISVEGEATNVVRSAAGHVYF
                     SIKDARAQMRVVLFAMNARFLRFRIENGLQLVVRGRLTLYEQKGEFQLNAVSVEPAGL
                     GALQLAFEQLKKQLEEEGLFDPSRKKTIPMLPQRIAIVTSPTGAAIRDVLNVLGRRFA
                     GLHVQVYPVRVQGREAGREIAAAIRNLSRWHLHDVVLICRGGGSLEDLWAFNEEIVAR
                     AVAACAVPTISAIGHETDFTICDFVADLRAPTPSAAAEIVIRAKSEICSQLDHMARRV
                     RQVVETRVRNYRHELRHLASSDRLGLLPRRIALRRERLNRSRVTLFRMLEARARSMRA
                     RLLAVSEPLLRFPAHIARERQRLRATAATLDAVSPLSVLSRGYAIAFSRTKRGRRKPI
                     LDSNVVTVGEPIEVQLKKGRLECTVDAKTMGLETVWPTSVKE"
     gene            2081..2344
                     /gene="xseB"
                     /locus_tag="VNN25_15595"
     CDS             2081..2344
                     /gene="xseB"
                     /locus_tag="VNN25_15595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXH93005.1"
                     /translation="MARKSNEFEKAFQQLETIVKRLESEEMPLDESLQLFEEGIRLSR
                     FCNQKLEEVEKKIELILSDAKGEPRVEPFESEEDDDLGEAPEE"
     gene            2341..>2851
                     /locus_tag="VNN25_15600"
     CDS             2341..>2851
                     /locus_tag="VNN25_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXH93006.1"
                     /translation="MSGINEWLSATRLVVDRVLDANLPEAETPPAVIHEAMRYAVLGG
                     GKRIRPILGIAAAEACGAHVDALLIPIAALELIHTYSLVHDDLPALDNDDLRRGRKTT
                     HVVFGEAMAILTGDALLTEAFSWLARPVAGVEARHQLRAVQEVALAVDSKGMIGGQVA
                     DLIATGKNEA"
BASE COUNT          515 a          876 c          886 g          574 t
ORIGIN      
        1 caaaagggcg cggtcgcccg cgccctgccg ttactgcgtt taccggggat tagtccttgt
       61 gcatcttgcg gtgatgcttc ggtgccggtg cctctggggc gggggtctcg actgtgctgc
      121 tcgaagccat cggggtttcg actaccgggg ccggggccgg agccggggct ggcgtctcta
      181 cgacgaccgg agccggggcc ggcgtctcaa cgaccatcgg ctcgaccggg gccggcgtgt
      241 tgctcacgac ggtcgtggtc gtcgactcga tgggcgtctg ggtgacgaca acttcagagg
      301 atgaggtctt cttggggatc aggttgacat tcgtattgtt gccgaggttg gaagcgttgc
      361 cgttgccgcc agcggaactg ctggtgtatg cacggccgtc gctgtcaacg gctgccgggc
      421 cggaaatcac ggcaggagtc gtcgagccac tcaccccttc cgcgttcttc ggcgcaggag
      481 cggttccgac cacaggagtg ctgttgtctg ccgtcgttgc tgccccttcg gtgcgaatct
      541 gaccgtatcc ctctgcctgc gatgctgcat cgtgatgtgc acacgcgctg agtgcgattg
      601 cgagcgcggc tgagccgatg cccgccacta ctttcttgaa gatcatttct tttttcattt
      661 tcctgactcc ttgctgcctt cagattgaca gctcgtctat tgccttgtcg gcaacgatca
      721 caagagcaac tagcttgcct agttttgatt gactaccatg tgcggatggc agatcgcgtc
      781 tggcaggtca gcgagctgat ccggcaaatc aacctggatc tctttcgtta caacgacatc
      841 agcgtcgaag gcgaagcgac aaacgtcgtt cgatcggctg ccggtcacgt ttatttttcg
      901 atcaaagacg cgagggcaca gatgcgcgtg gtcctttttg ccatgaatgc gcgcttcctt
      961 cgcttccgaa tcgagaacgg attgcaactc gtcgttcgtg ggcgcctaac gctttacgag
     1021 caaaaaggtg agtttcaact caatgccgtg tctgtagagc ccgctggtct aggcgctctg
     1081 caattggcgt tcgagcagct caagaagcaa ctcgaggaag aagggttgtt tgatccatcg
     1141 cgcaaaaaga cgatccccat gctgccccag cgcattgcca tcgtcaccag tcctaccggt
     1201 gcggccatcc gcgacgttct caacgtcctc gggcgccgct tcgccggtct gcacgtgcag
     1261 gtttacccgg tgcgtgttca ggggcgcgag gcgggacgag agatcgccgc ggcaatccga
     1321 aatctttccc gttggcatct gcacgacgtc gttctcatct gccggggcgg aggctcactc
     1381 gaggacttgt gggcgttcaa tgaagagatt gtcgccaggg ccgtcgccgc gtgcgcggtt
     1441 ccgaccattt cggcaatcgg tcacgagacc gacttcacga tctgtgattt cgttgctgac
     1501 cttcgcgcac ccactccctc ggccgcggcg gagatcgtga ttcgtgcgaa aagcgagatc
     1561 tgctcgcagc tcgaccatat ggcgcggcgc gtgcggcagg tggtcgaaac gcgcgtccgc
     1621 aactaccgcc acgagcttcg ccacctcgca tcctcggatc gcctcggctt gctgccgcgc
     1681 cggatcgctt tgcgccgcga acgcctcaac cgcagccgcg tcacgctgtt tcgcatgctc
     1741 gaagcccgcg cccgatcgat gcgcgcacgt ctgctcgccg tcagcgagcc actgctgcgc
     1801 tttcccgcgc acatcgctcg cgagcgtcaa cgtctccgcg caacggcggc tacgctcgac
     1861 gccgtctcgc cgctctccgt gctctcacgc ggctacgcca tcgcgttctc ccgcacgaaa
     1921 cggggacggc gaaagccgat cctcgattcg aatgtggtaa cagtcggcga accgatcgag
     1981 gtgcaactga aaaagggccg cctcgaatgt acggttgacg cgaagacgat ggggcttgag
     2041 actgtgtggc ccacatcggt caaggaatga gcaacgagta atggcccgca aatccaacga
     2101 attcgagaaa gcctttcagc agctcgagac gatcgtaaag cgtctggagt cggaagagat
     2161 gccgcttgac gagtcgctgc agctcttcga ggaggggatt cgtctctccc gtttctgcaa
     2221 tcagaagctc gaagaggtcg agaagaagat cgagctgatc ctgtccgatg cgaaagggga
     2281 gccgcgcgtc gaaccgttcg aaagcgagga agacgacgat ctcggcgagg cccccgagga
     2341 gtgagcggga tcaacgaatg gctgtcggca acgcggttgg tcgttgacag ggtgctggac
     2401 gcgaaccttc ccgaagccga aaccccgccc gcggtcatcc acgaggcgat gcgctacgcg
     2461 gtcctcggcg gcggaaaacg catccgccca attcttggaa ttgcagcagc ggaagcgtgc
     2521 ggagcgcacg tcgacgcgct gctcattccc attgccgcgc tcgaactgat ccacacctac
     2581 tcgctcgtcc acgacgatct tcccgcgctc gacaacgacg acctgcgccg cggccggaag
     2641 accacgcacg tagtcttcgg cgaagccatg gcgatcctta ccggcgacgc gcttctgacc
     2701 gaggcgtttt cgtggctggc gcgtccagtg gcgggagttg aggccaggca tcagcttcgc
     2761 gctgtgcagg aagtcgccct cgccgtcgac tcgaagggca tgatcggagg tcaggtcgcc
     2821 gacctcatcg ccacggggaa gaacgaggcc a
//
LOCUS       DATLVW010000522         4041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_433509, whole genome shotgun sequence.
ACCESSION   DATLVW010000522 DATLVW010000000
VERSION     DATLVW010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433509"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNN25_15605"
     CDS             complement(<1..216)
                     /locus_tag="VNN25_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93007.1"
                     /translation="MTATVPASKAKAVRQQKYFITTPDESPINVYSAAMSKGMQALKK
                     YRLAENVPNVMTAALSEKEVERLVKFGA"
     gene            490..2112
                     /locus_tag="VNN25_15610"
     CDS             490..2112
                     /locus_tag="VNN25_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93008.1"
                     /translation="MKRFDALVAIVLLFISSIAVAQEARPQPSEGPAAMLALVVTDSK
                     GMHVPSLSKDDVQLSIGGVPVDLATFAERGVGGTPAREVRRIAVLFQVSSLSMSARRQ
                     ASEAIHGFLARTLRPGDLAVVLAGDQTLRAMTAWTSKLPAIDAALERVSGESAPSLTD
                     GQTAAEKRIREIATDIQQADAKGHTLYTFDALLDSARAYASARYRDAELSLGNISSVV
                     SLFTPRTRNVLIVVGADLPRTPGAGVFQYVEGLRTSALRGARGAAMQKGAQFSSPMGE
                     SSAYDLTSLFDSVGTRFWRRGVAVYAISSEMRKDSSGGSETSQTADALAAFTNEADRV
                     AGYRLLSGATGGIAFIGRSPADALDRIASDLESFYTVGVHPTAPISGKDALSVKVRNG
                     YSVRVTRGSAGLGTPTDEMESRVIAYQQVNPADENALGISLHAAPPVFDGERRLVSVD
                     VIIPIRNLTMVKVGNDNASAFTVFIATGDPLGNSSNVNTQRKLIRWPDGVTPASALTF
                     RVNVVLEPGRSQISIGVMDEHSHEKGFDRVTI"
     gene            complement(2482..3312)
                     /locus_tag="VNN25_15615"
     CDS             complement(2482..3312)
                     /locus_tag="VNN25_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93009.1"
                     /translation="MKTAGNYVLTPQGLVHKSRIHSAEQIGASSAPWPAPNRFPPSTT
                     VMQALSPNGWKASATFAAAVALGKMSVKFKVPEPPAMDGALIYLFSGAENAQMRTILQ
                     PVLQWGDNGAYGGECWKISCWHASVDGQWYYSTPVDVSTGDIVTGTVEVVEGPAKDCT
                     AKDCTCTWKIIASTDKGASTMLDESMDPSLAMSSLMLFLIGAALEAYPEEACCTGIAR
                     EQYPASDTVFTDIELTDLAGYRFLADWNSRCDLGDNALCGCKNTVQINPDKSTITLRY
                     "
     gene            complement(3343..>4041)
                     /locus_tag="VNN25_15620"
     CDS             complement(3343..>4041)
                     /locus_tag="VNN25_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93010.1"
                     /translation="DGRYLKIVNATGERLHFSRKEGFSLNEFVYDLQLDDDVVFAGNA
                     ACYFDIFHGCAWTEGIGGEPRTTRVRMETEGKPRLAIAAMPRSGPDVCGDYEIEIQPN
                     AAGTYELFVTNLDYDPATEYRNFDFLMNYLVARGGIPRVLKKPTPGMPPDPDELTMRY
                     LGEKLKALGEFIRTDGGTVGGYFDAGGGIKLEGGSTNPAPSKARHSSLAERLAAGVQA
                     PVPLDESCSDSHVP"
BASE COUNT          771 a         1238 c         1214 g          818 t
ORIGIN      
        1 ggcaccgaac ttcacgagcc gctccacctc tttctcggaa agggctgcgg tcatgacgtt
       61 cgggacattc tccgccaaac ggtacttctt cagcgcctgc atacccttcg acattgcagc
      121 ggaatagacg ttgataggcg actcgtcagg ggtagtgatg aagtacttct gttgtcgaac
      181 ggctttcgcc ttcgatgcag gaacagtagc ggtcatagtt ctcccctgca gtggggagag
      241 ccaccaggtc accgtttccc tacccaacga tcgctcgaac agcaatccct ttgacgagat
      301 gcgcgacgcg tcggtggtca catggtttcg cgcaggaaag gtaccgtggt acgcgggcga
      361 ggacggtctt tactccccta tcgagcttcg gtgccgatgc gccgcaccca tgcgaagagg
      421 cgtagacttc ctcgccagcc cggtttgcaa tgcagcgtcc cggacgttca atcgaacacg
      481 gaggacctca tgaagcgttt cgatgccctg gtcgcgatcg tcctcctctt catttcatca
      541 atcgcagttg cgcaggaggc gcggccgcaa ccgagcgagg ggccggcggc gatgctggcg
      601 ctcgtcgtga ctgattcgaa agggatgcac gttccttcgc tatcgaaaga cgatgtccag
      661 ctctccattg gcggcgtgcc ggtggatctg gccacgttcg ccgagcgagg agtgggcgga
      721 acgccggcgc gcgaagtgcg caggatcgct gtgctgtttc aggtgagctc tctatcgatg
      781 tccgcgcggc gtcaggcgtc cgaggcgatc cacggatttc tggcgcgcac actgcggccg
      841 ggtgatctcg cggtcgtcct cgccggcgat cagacgttgc gcgccatgac ggcgtggaca
      901 tcgaaactgc cggcaatcga cgccgcgttg gagcgggtgt cgggcgagtc ggcaccttcg
      961 ctcacagacg ggcagaccgc cgccgaaaag cgaattcgcg agatcgctac ggacatccag
     1021 caggcggacg cgaaggggca tacgctttac acgttcgacg cattgctcga ttcagcgcga
     1081 gcctatgcat ccgcccgcta ccgcgatgcc gagctgtccc tcggcaacat ctcatcggtg
     1141 gtaagcctct tcacgccgcg cacgcgcaac gtgctcatcg tcgtcggcgc cgaccttccc
     1201 cgcacgccgg gcgccggagt gtttcagtac gtggagggat tgcgcacatc ggcgttgcgg
     1261 ggcgcccgag gggcggccat gcagaaggga gcgcagttca gctcaccgat gggagagtcc
     1321 tccgcctacg atctgacgtc gctgttcgac tccgtcggta cccgcttctg gcgccgcggc
     1381 gtggccgtct atgcgatcag ctccgagatg cgcaaagact cgtcaggcgg cagcgaaacc
     1441 agccagaccg ccgatgcgct cgcggcattc acgaacgagg cggaccgcgt cgccggttac
     1501 cgcctgcttt ccggagcaac cggcggcatc gccttcattg ggcgttcccc ggccgatgcg
     1561 ctcgaccgca tcgcctcgga cctcgagtcg ttctatacag tgggcgttca tccgacagcc
     1621 ccgatttccg ggaaggatgc gctgtcggta aaggtccgca atggctattc ggttcgtgtc
     1681 acgcgcggca gcgcaggatt aggaacgccg acggatgaaa tggaaagccg cgtcattgcc
     1741 tatcaacagg tcaacccggc cgatgagaat gcgctcggca tctctcttca tgccgcgccg
     1801 cctgtgttcg acggcgaacg gcgcctggtt tccgtcgacg tcatcattcc cattcgcaac
     1861 ttgacgatgg tgaaggttgg aaacgacaac gccagcgcct tcaccgtctt catcgccacc
     1921 ggcgaccccc tcggcaactc ctcaaacgtc aacactcaac ggaaactaat ccgctggccc
     1981 gacggcgtca cgcccgcgag cgcgttgaca ttccgcgtca acgtcgtcct cgagcccggc
     2041 cgctcccaga tctccatcgg cgtaatggac gagcactcgc acgaaaaggg attcgatcgg
     2101 gtgacgattt aggctttacg gcgtagagga gtccgaacgg atcggagtga cagtccaatc
     2161 gagattggtg tcatcctgag actgccggag ccgcccgcgc gaatcgcgat gtttgctcag
     2221 tgcgtgctcg gcggcggagg acggcgaagg atctcaaact acgcagatcg tgttcgccca
     2281 gcgcgagcga tcgtaattaa agacgctcga gcgagatctt cggagccgcc cgagagtgtg
     2341 agaaatgccg gctaacgcgt ctgctgccac atgttggtag tgggtcaatt tgaaaaaccg
     2401 gtcaccacag agacacagag gtcacagagt tcctctctgt gttctctggg tctctgtggt
     2461 tgcctttgct tcggccagca gtcaataccg cagcgtaatc gtgctcttgt cggggttgat
     2521 ctgcaccgtg ttcttgcagc cacagagcgc gttgtccccc aggtcgcatc tcgagttcca
     2581 gtcggcgagg aatcgatatc ccgccagatc ggtaagctcg atgtcggtga ataccgtgtc
     2641 gctagcagga tattgctcac gcgcgatacc cgtgcagcac gcttcctccg gataggcttc
     2701 gagcgctgcc ccgatgagga atagcatcaa tgaagacatc gccagcgaag ggtccatgct
     2761 ctcatcgagc atagtggacg cccccttatc ggtagaagcg atgatcttcc acgtgcacgt
     2821 acaatccttg gcggtgcagt ccttggcggg accctccacc acttccaccg ttccggtgac
     2881 gatgtcgccg gtgctcacgt ccactggtgt cgagtaatac cactggccgt ccacggaggc
     2941 atgccaacat gagattttcc agcactcgcc tccataggcg ccgttatcgc cccactgaag
     3001 cacgggctgc agaatcgtcc tcatctgggc gttttccgct cccgaaaaca ggtaaatgag
     3061 cgctccgtcc atagccggcg gctcgggtac cttgaacttg acgctcatct tgccaagggc
     3121 aacagccgca gcgaacgtcg ccgacgcttt ccagccgttg gggctcagcg cctgcatcac
     3181 ggtagtgctt ggggggaatc gattcggtgc cggccaaggc gcgctgctcg cacctatctg
     3241 ttcagccgaa tgaatccgcg atttgtgcac caggccttgg ggcgtcagga catagtttcc
     3301 agcggtcttc atggtcactc ctctcaaggg aaaatggctg ttttagggga cgtggctgtc
     3361 agagcaagac tcgtcgaggg gcacaggagc ttgaacgccc gcggcaagcc tctcggctag
     3421 agatgaatgc ctggcctttg agggggcagg attcgttgac ccgccttcaa gcttaatccc
     3481 gccgccagcg tcaaagtagc cgccaacagt gccgccatcg gtcctgatga attcgcccag
     3541 cgctttcagt ttttctccga gatacctcat ggtcagttca tccggatcgg gaggcattcc
     3601 gggagtaggt ttcttcagca ctcttggaat gccgcctctt gcgacgagat agttcatgag
     3661 gaagtcgaaa ttgcgatatt cggtggccgg gtcatagtcg agattcgtga cgaacagctc
     3721 atacgtgcca gcagcattgg gttggatctc gatctcgtag tcgccgcaga cgtccggacc
     3781 tgaacggggc atggccgcga tggcgaggcg cggtttccct tcggtttcca tccgcacgcg
     3841 ggtggttcgc ggctcgcccc ctatgccctc cgtccacgcg cagccgtgga agatatcgaa
     3901 atagcaagcg gcgttgccgg cgaaaacgac atcgtcgtcc agctgtaagt cataaacgaa
     3961 ttcgttcagc gaaaaaccct ccttacggga aaagtgcagg cgctcgcccg tcgcattgac
     4021 aatctttagg taacggccgt c
//
LOCUS       DATLVW010000523         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_356903, whole genome shotgun sequence.
ACCESSION   DATLVW010000523 DATLVW010000000
VERSION     DATLVW010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356903"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2165)
                     /locus_tag="VNN25_15625"
     CDS             complement(<1..>2165)
                     /locus_tag="VNN25_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93011.1"
                     /translation="ELIGVWHSAPGVNIAELNMAPSNYFIYREQNQTFQDIGVYQRDA
                     VSITGLAEPERLDALDVTDGILPLLAIPPMLGRSFTRQDDSPGAPDTVMLTYGFWKKK
                     FAGSTSVIGQSVTLDGKSREIIGVLPQRFHFLDWTDPAVVLPFKWDRNKVNLGNFSYE
                     GIARLKPGVTMEQASADVARMLPIVSHSFPAPPGFSAKLFEDAHIGPNLRPLKIDVIG
                     DVGSTLWVLMGSIGLVLLIACANVANLLLVRVEGRRQELAVRSALGASPYRIAGELLL
                     ESVTLGFLGSAIGLGLAYTALRILVAIAPDGLPRINEIGIDGPVLLFTFAVSILASAL
                     FGSIPIFKYAGVRLSTGIRQGGRANSGSREQHRARSILVVVQVALALVLLICSGLMIR
                     TFRALTHVNPGFSQPAQLQTFRIGIPDNQVMDDESVVRTFEEMRAKIAAIPGVSAVTI
                     STSFPMDGNSNNDLVFAEDHPYMEGKIPPVRRFAFVIPGFFATEGTPLVAGRDFTWTD
                     IYQKLPVAIISENFAREYWDSPANAIGKRIRVASTDDWREIVGVAANVYRDGVSKNPP
                     PMVYWPIMRAKFEGQAHDVARYTAFGIRSPRAGSEAFLNEVRAAVWSVNPNLPLSRVH
                     TLDYYYTRSMARTSFTLIMLAVAGGMALLLGVVGIYGVIACSVSQRVREIGIRMALGA
                     QTNSLTGMFVRHGLILTGIGAAFGLVAAAIVMRRYRRGS"
BASE COUNT          491 a          531 c          755 g          388 t
ORIGIN      
        1 ctgatcctct ccggtatcgg cgcatgacga ttgcggcggc gacgaggccg aaggctgcgc
       61 cgataccggt gaggatcagg ccgtggcgga cgaacatccc ggttagcgaa ttcgtctgcg
      121 caccgagagc catgcgaatg ccgatttcgc ggacgcgctg ggagacggag caagcgatga
      181 cgccatagat gccgacgacg ccgagtagaa gggccatgcc gcctgcgacg gcgagcatga
      241 tgagggtgaa agaagtgcgg gccatggaac gggtgtagta atagtcgagg gtatgcactc
      301 tcgagagagg aagattggga ttcacggacc agacagctgc gcgaacttcg tttagaaagg
      361 cctcggaacc ggcgcgcggg ctgcgaatgc cgaaagcggt gtatctggcg acgtcgtgcg
      421 cctggccttc gaacttagcc cgcataatgg gccaataaac catggggggt ggattcttgc
      481 tgacgccgtc gcggtaaacg ttggcggcga cgccaacgat ttcgcgccag tcatcggtgg
      541 aagcgacacg aatgcgtttg ccgatggcgt tggcgggaga gtcccaatac tcgcgagcga
      601 aattttcaga gatgatggcg acgggtagct tctggtaaat gtccgtccag gtgaagtcac
      661 gtcctgcaac gaggggagtg ccctcagttg cgaagaaacc cggaatgacg aacgcgaacc
      721 tgcggaccgg aggaattttc ccctccatgt acgggtgatc ctcggcgaag accaggtcgt
      781 tgttggagtt gccatccata ggaaaagagg tggagatagt gaccgccgaa acgccaggaa
      841 tcgcggcgat cttagcgcgc atttcttcga acgttcggac gacgctctcg tcgtccatga
      901 cttgattgtc aggaatgccg atgcggaagg tttgcagttg cgcgggctga gagaagccgg
      961 gattgacatg tgtgagcgca cggaatgtcc ggatcatgag tcccgagcag atgaggagaa
     1021 cgagagcgag ggcgacttgc acgacgacga gaatgctgcg agcgcgatgt tgttcacggc
     1081 ttccgctgtt ggcacggccg ccttggcgaa tgccagtgct gagacgaacg cctgcgtatt
     1141 tgaagatggg aatggagccg aagagcgcgc tggcgagaat ggagacagca aaggtgaaga
     1201 gcaggacggg gccgtctatg ccgatttcgt tgatgcgggg caggccgtca ggggcaatgg
     1261 cgacgaggat acgcaaggcc gtgtaagcca gtcccaaacc gatcgcgctg ccgaggaaac
     1321 cgagagtaac actttccagg agaagttcac cagcgatacg gtaggggctc gcgccgaggg
     1381 cggaacgaac agctagttcc tgacggcggc cttcgacacg cacaaggagg aggttcgcga
     1441 cgttggcgca agcgatgagt agtacaaggc cgatggagcc catgagtacc cagagcgtgc
     1501 tgccaacgtc gccgatgaca tcgattttca gcgggcggag gttgggaccg atgtgggcgt
     1561 cttcaaacag cttggcgctg aatccgggag gagctggaaa cgaatgggaa acgatgggaa
     1621 gcatgcgggc cacgtcggcg ctggcctgtt ccatggtgac accgggcttg aggcgggcga
     1681 taccttcata gctgaaattg ccgagattga ccttgttgcg atcccatttg aagggaagga
     1741 cgacggcggg atcggtccaa tcgagaaaat gaaagcgctg cggaagcacg ccgatgatct
     1801 cgcgcgactt gccatccagt gtgacggact ggccgatgac ggaggttgag ccagcaaatt
     1861 tctttttcca aaagccgtag gtgagcatga cggtgtcggg cgcaccaggc gagtcgtcct
     1921 ggcgggtgaa ggagcggccc aacatggggg gaatggcgag caagggaagg atgccgtcgg
     1981 tgacgtcaag agcgtcaagg cgctcgggtt cggccaagcc ggtaatgctg actgcatcgc
     2041 gctggtagac gccgatatcc tggaaggttt gattttgttc acgatagata aagtaattag
     2101 agggcgccat gtttagctcg gcgatgttga cgcccggcgc ggagtgccag acgccgatca
     2161 attcg
//
LOCUS       DATLVW010000524         4439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_558406, whole genome shotgun sequence.
ACCESSION   DATLVW010000524 DATLVW010000000
VERSION     DATLVW010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558406"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VNN25_15630"
     CDS             complement(<1..668)
                     /locus_tag="VNN25_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH93012.1"
                     /translation="MAVDALGRPLRDLRISVTDRCNFRCTYCMPKEVFGRDFAFLDRA
                     ELLTFEEITRLAQLFVREGVAKFRLTGGEPLLRRDIERLVAMLSRIRGVEDIALTTNG
                     ALLTREKALALRNAGLNRITLSLDSLDDAVFRRMNDVEFPVARVLDAIDAASDAGLTP
                     IKIDMVVKRGENDDSIVAMASRFRGTGHIVRFIEYMDVGNTNGWRMEDVVSAREIAER
                     ISDQ"
     gene            complement(658..1521)
                     /locus_tag="VNN25_15635"
     CDS             complement(658..1521)
                     /locus_tag="VNN25_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXH93013.1"
                     /translation="MRDTLRATFLRVLRLDNRRKEIRRGSLDHIRSFHSDILGNERDI
                     TIYLPPGYHDRDELRYPVLYMHDGQNLFEPERAFIPGQHWRLAEAADEVIGGRTARPM
                     IIVAIDNTGPSRIDEYTPTRDIKHHGGGKASDYGRMLIVELKPVIDAKFRTLTDPLNT
                     ALGGSSLGGLATLHLGLKHPDFFGGLAVMSPSVWWDNRIIFDDVERFDGRNRPRIWLD
                     MGGREGVEGLANARMLRDRLIARGWRIGVDLRYFEDRRADHSERSWAKRAPAVLEFLF
                     PPVELSTPNGS"
     gene            complement(1530..2003)
                     /locus_tag="VNN25_15640"
     CDS             complement(1530..2003)
                     /locus_tag="VNN25_15640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXH93014.1"
                     /translation="MPIDDAHFKLALSHFASGVTVVTTEHEGRPFGMTVASFASLSLN
                     PKLVLVCIETSVKTHEAIVAAGKFGVSILASEQSGISSKFASRSDDKFTGVDIFRGAL
                     DLPMITGALTTIECKVQAQLPGGDHSIFVGEVIDIQTREGDPLLYFRSGYREMRS"
     gene            2088..3251
                     /gene="sucC"
                     /locus_tag="VNN25_15645"
     CDS             2088..3251
                     /gene="sucC"
                     /locus_tag="VNN25_15645"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXH93015.1"
                     /translation="MKVHEYQAKEILRRYGVPTPRGIVVDNPEAARTAAKELGGRVVV
                     KAQIHAGGRGKGGGVKLAKDPDEAVELAKKIIGMTLVTHQTGPEGRVVGKVLIEEALN
                     IDKELYLGITLDRRIGMPVIMASKQGGMEIEEVAERDPSAIHREPIDGVLGTLPFQAR
                     AVANALGLTGDSYKKCIDFVQKLMRCYIEMDASLAEINPLLVTKEGDVLALDCKMNFD
                     DNAMFRHKDVAEMRDLKEEDPLEVEASEFGLNYIKLDGNVGCMVNGAGLAMATMDIIK
                     LAGGQPANFLDVGGGASAEQVRNAFRIILSDANVKAILINIFGGIMRCDRIAEGVVSA
                     SREVGLPVPVVVRLEGTNVELGKEILAKSGLPLITADGMWDAAQKVVAAAQGK"
     gene            3263..4162
                     /gene="sucD"
                     /locus_tag="VNN25_15650"
     CDS             3263..4162
                     /gene="sucD"
                     /locus_tag="VNN25_15650"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXH93016.1"
                     /translation="MAIWADKNTRVIVQGITGREGTFHAIQCREYGTNMVGGVTPGKG
                     GTTHEGFAVFNSVREAREKAGANCSLIFVPPPFAADAVMEAADAGIELIICITEGIPV
                     NDMIRAHDFLKKSNARAIQSGATPSRLIGPNCPGIITPGEAKIGIMPGHIHRPGPIGI
                     VSRSGTLTYEAVGQTRALGQSTAVGIGGDPLPGTNFIDCLAAFENDPATEAIIMIGEI
                     GGSAEEEAAAFVKANVTKPVVAFIAGQTAPPGRRMGHAGAIISGGKGTASEKMAALKA
                     AGIHVVEAPSEMGAKVAEVWKGR"
BASE COUNT          873 a         1335 c         1456 g          775 t
ORIGIN      
        1 cactgatcgg aaatgcgctc ggcgatctcg cgcgcggaga cgacgtcttc catccgccag
       61 ccgttcgtgt tgccgacgtc catgtactcg atgaaacgga cgatgtggcc ggtgccgcga
      121 aaccgcgagg ccattgccac gatgctgtcg tcgttctccc cgcgtttgac gaccatgtcg
      181 attttgatcg gagtgagacc ggcgtcggac gcggcatcga tcgcgtcgag cacgcgcgcg
      241 acggggaact cgacatcatt catccgccgg aagaccgcgt cgtcgagcga gtcgaggctg
      301 agcgtgatgc gattcaggcc ggcattgcgc agggccagcg ccttttcgcg ggtgagcaac
      361 gcaccgttcg tcgtcagcgc gatgtcttcc acgccgcgaa tgcgcgacag cattgccacg
      421 agccgctcga tgtcgcggcg gagaagcggc tcgccgccgg tgagcctgaa cttcgcaacg
      481 ccctcacgca cgaagagctg tgccagacgc gtgatctcct cgaaggtcaa caactccgcg
      541 cgatcgagaa aggcgaagtc acggccgaag acctctttcg gcatgcagta cgtgcaacgg
      601 aagttgcacc ggtcggtgac agaaatccgc agatcgcgaa gcgggcgtcc tagggcgtca
      661 actgccattt ggggtggaca actcgaccgg cgggaacaaa aactccagca ccgccggggc
      721 gcgtttggcc caggagcgct cgctgtggtc cgcgcggcgg tcttcgaagt aacggaggtc
      781 gacgccgatg cgccagcctc gtgcgatcaa tcggtcgcgc agcatgcgcg cattcgcgag
      841 cccttcgaca ccctcgcgcc cgcccatatc gagccagatg cgtgggcgat tgcggccgtc
      901 gaagcgctcg acgtcgtcaa aaatgatgcg gttgtcccac cagacggagg gcgacatcac
      961 ggccagaccg ccgaagaagt cggggtgttt cagtccgagg tgaagcgtgg ccagaccgcc
     1021 gagcgatgag ccgccgagtg cggtgttcaa gggatccgtc agcgttcgga actttgcatc
     1081 gatgacgggt ttcagctcta cgatcagcat ccgaccgtaa tcgctcgctt tcccgccgcc
     1141 gtggtgcttg atgtcgcggg tcggcgtgta ttcatcgatg cgcgaaggtc cggtgttgtc
     1201 gatggcgacg atgatcatag gccgcgccgt ccgtccgccg atgacttcgt ccgccgcctc
     1261 ggcaagccgc cagtgctggc cgggaatgaa cgcccgttcc ggctcgaaga ggttctgccc
     1321 gtcgtgcatg tagagcacgg gataacgcag ctcgtcgcgg tcgtgatagc cgggcggcag
     1381 gtagatggtg atgtcgcgct cgttgccgag gatgtcggag tggaagctgc gaatgtgatc
     1441 gagcgatccg cggcggattt ctttgcggcg attgtcgagc cgcagtacgc gaaggaacgt
     1501 cgcgcgcaat gtgtcgcgca tcggctgcat cagcttcgca tctcgcgata gccgctgcgg
     1561 aaatagagga gaggatcgcc ctcgcgcgtc tggatgtcga tgacttcacc gacgaagatg
     1621 gagtgatcgc cgccgggcag ctgtgcctgc accttgcact cgatcgtcgt gagcgctccc
     1681 gtgatcatcg gtagatcgag cgcgccgcga aagatatcga cgccggtgaa cttgtcatcg
     1741 ctgcgtgatg cgaatttcga ggagatgccg ctctgttcgc tggcgaggat gctgacccca
     1801 aacttgccgg cggcaacgat cgcctcgtgc gttttcaccg acgtttcgat gcagacgaga
     1861 acgagtttgg gattgagcga cagcgacgcg aacgacgcca cggtcattcc gaatggccgc
     1921 ccttcatgct cggtcgtgac gaccgtgact ccgctcgcga agtgcgacaa ggccagtttg
     1981 aaatgagcgt catcgatcgg catcgggtac ctccgggcgc ggcagtttaa tccaacttcc
     2041 gggcgtgtcc gcgcatcgtc gttcaatgtg ataaaagtcg ccgccctatg aaagttcacg
     2101 agtatcaggc caaagaaatc ctgcgccgtt acggcgtgcc gacgccgcgc ggaatcgttg
     2161 tcgacaatcc cgaagcggcg cgcactgccg cgaaagagct gggcggccgc gtcgtcgtca
     2221 aggcgcagat ccatgcgggc ggccgcggca aaggcggcgg cgtcaagctg gccaaagatc
     2281 ccgacgaagc cgtggagctt gcgaagaaga tcatcggcat gaccctcgtc acgcaccaga
     2341 ccggccccga aggacgcgtc gtcggaaagg tgctgatcga agaagcgctc aacatcgaca
     2401 aggagctcta cctcggcatc acgctcgacc gccgcatcgg aatgccggtg atcatggcgt
     2461 ccaaacaggg cggcatggag atcgaagagg tggccgagcg cgatccgtcc gccattcacc
     2521 gcgagccgat cgacggcgtc ctcggcacgc tgccatttca ggcacgcgcc gtcgcgaacg
     2581 ccctcggcct caccggcgac tcgtacaaga agtgcatcga cttcgtccag aaactgatgc
     2641 gctgttacat cgagatggat gcgtcgctcg ccgagatcaa tccgctcctt gtgacgaagg
     2701 aaggcgatgt tctcgcgctc gactgcaaga tgaacttcga cgacaacgcg atgttccggc
     2761 acaaagacgt cgccgagatg cgcgatctga aggaagagga tccgctcgaa gtggaagcgt
     2821 cggagttcgg gctcaactac atcaagctcg atggcaacgt cggttgcatg gtcaacggcg
     2881 ccggcctggc catggccacg atggacatca tcaagctcgc cggcggacag cctgcgaact
     2941 tcctggatgt cggcggtggt gcgagcgcgg aacaggtccg caacgcgttc cgcatcatcc
     3001 tctccgacgc taacgtgaag gcgatcctca tcaacatctt cggcggcatc atgcgttgcg
     3061 atcgcatcgc cgagggggtg gtgagcgcgt cgagggaagt ggggctgccg gttccggtcg
     3121 tcgtgcgcct cgaagggacg aacgtcgagc tcgggaaaga gatcctggcg aagtcgggac
     3181 tgccgttgat caccgccgat ggaatgtggg acgccgcgca gaaagtcgtc gccgcagcgc
     3241 agggaaaata gagaccaaca ctatggctat ctgggcagac aagaacacac gagtgatcgt
     3301 gcagggcatc accggccgcg aggggacgtt tcacgccatt cagtgccgcg aatacggaac
     3361 gaacatggtc ggcggcgtga cgccgggcaa gggcgggacg acgcatgagg gtttcgcggt
     3421 cttcaacagc gttcgcgaag cgcgcgagaa agcgggcgcg aactgctcgc tgatcttcgt
     3481 gccgccaccc ttcgcggcgg acgcggtcat ggaagcagcg gatgccggca tcgagctgat
     3541 catctgcatc acggaaggga ttccggtcaa cgacatgatt cgcgcgcacg actttctgaa
     3601 gaagtcgaat gcacgcgcga ttcaatcggg cgcaacgccg tcacgcctca tcggtccgaa
     3661 ttgccccggc atcatcacgc ccggcgaggc gaagatcggc atcatgccgg gccacatcca
     3721 tcgcccggga ccgatcggca tcgtctcgcg ctcgggaacg ctgacgtatg aggctgtcgg
     3781 ccagacgcgc gcgctcggtc agtcgacggc agtcggcatc ggcggcgatc cgctgccggg
     3841 aacgaacttc atcgactgtc tggccgcctt cgaaaacgat ccggccaccg aggcaatcat
     3901 catgatcggc gagatcggcg gctcggcgga ggaagaagcc gcggccttcg tgaaggcgaa
     3961 cgtaaccaag ccggttgtcg ccttcatcgc cggccagaca gcgccaccgg gccgccgcat
     4021 gggccacgcc ggcgcgatca tcagcggcgg caaagggacg gcgtcggaga agatggccgc
     4081 gctgaaggca gccggaattc acgtcgttga agcgccaagc gagatggggg cgaaggtggc
     4141 ggaggtttgg aaggggcggt aggtaggggt ctcgcgcttg tcgttgcttg agtcaggaat
     4201 taaggtcaat cggttcgacc ggatggcctc gttgctcggt aatttcctct tcgatgccga
     4261 cgccatgaaa aatagccgcg agctgtgatc cgaaacccac gcgagtgccg tcctcgttct
     4321 tgaccgcgtt gcgaatgatt tcgcgaacct cttcatccaa gctgcggccg tgtgctggac
     4381 gcacgctgcc gaagcttttc gatcacatcc tcgagctcaa tcacgagctg atccgcggg
//
LOCUS       DATLVW010000525         5349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_356971, whole genome shotgun sequence.
ACCESSION   DATLVW010000525 DATLVW010000000
VERSION     DATLVW010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356971"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VNN25_15655"
     CDS             complement(<1..565)
                     /locus_tag="VNN25_15655"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93017.1"
                     /translation="MLRIALAFALSLAVAVTDVTAGDTVTIRSAIDSLSHGKIVMIEG
                     DAICASRPLPLFYSRRQFAPAWSARDRGELLAAIRDAKSDGLDPADYHLAAIERASDN
                     EQRDLLLTDAFFLLASHLLSGRVDPESIEPTWCLTPRANDLVAALETALELHDVRAAI
                     ARMPPAHPEYAALRRWLATYRRIAAAGG"
     gene            665..1216
                     /locus_tag="VNN25_15660"
     CDS             665..1216
                     /locus_tag="VNN25_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH93018.1"
                     /translation="MTHTIGAGLRPSPPHVLHAAVLKRAATHLLSMMKVRDAMTPDPA
                     TCDAATTLRLVAMLMLDHDCAAVPIVSGDEIVGIVTDRDIACRAVARGWNAADLPASA
                     VMTSPLVAINPDETFEDAIQVMRENHVHHLPVIDDDGRLVGIVAQSDLGRRMTNRELG
                     LLARETSIRRAGRGPAKLVRARA"
     gene            1241..2230
                     /locus_tag="VNN25_15665"
     CDS             1241..2230
                     /locus_tag="VNN25_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH93019.1"
                     /translation="MRSASSRFLRAAGAIATGATALAYTAYRKDIEAARRRIDLDRQI
                     IDTEQGRIEFAESGDGPAVLLIHGAGGGFDQGLDLGRAFLGDHYRIVAPSRFGYLGTP
                     LPADASAEAQAEAHLRLFDALQLDRVPVIGVSAGGPSAMQFCLRHPERCSALLLIVPL
                     AYAPQRAAVRVLSPIFETVLNTIAASDFLFWTATKVARMTLLKTILGTPVDAYRNATP
                     DERRSVDQMLRGILPISRRVAGIWNDSVVSTTLTRYQLEDIRVPSLVISAADDLYGTY
                     EGGLYTAEQIHNGTFIGFGSGGHLLLGHQAEVRSQITNFLERLETSGGTAMAI"
     gene            2350..2832
                     /locus_tag="VNN25_15670"
     CDS             2350..2832
                     /locus_tag="VNN25_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93020.1"
                     /translation="MKHYRLAVLLLAVLPLSAAAAPRAIPGINAKDVYPAGCIDCHTK
                     KAGGADTRISVLLARWNGSVDPKRLAAMQTFVPKGMTLKGKHPAVTAAVKDVPSSCVK
                     CHAAGSKTMPPLAPLMHGVHLAEGDQSEFVKQFQGECTYCHKLNKTSGTWLMPNGAEK
                     "
     gene            2909..3223
                     /locus_tag="VNN25_15675"
     CDS             2909..3223
                     /locus_tag="VNN25_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93021.1"
                     /translation="MHPDLEAIVSADEEARSRVSLAGKRREHDTLAARTARDATIDAH
                     LKKESDALDRELDGIRAEGDARIGELKKQQDQYLASLAAAGERKFDDAVALVLRMVCE
                     VA"
     gene            3223..4074
                     /locus_tag="VNN25_15680"
     CDS             3223..4074
                     /locus_tag="VNN25_15680"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HXH93022.1"
                     /translation="MRARTPSRGSLAYAHARIRAWKSRLLTRAGAAPLFAATDAAAIQ
                     RAIAALELDDPLRRLLRVYQTAIDGYSNGAPLFRAMLQRHEIENVKLLWRVVANGHRD
                     RVARLWIDLGAFASFPLPEAPTLNELVAQVAATPYAEIARSVARVHGTDLAAAELAFD
                     RWASRRLLEEARSLPKQESIARRLIELVVREREAQLVLRGAKWYGLASVAPGNITDIT
                     ALRRERLRFCHRAFIGRPFLLGSAVAVILLAEEEVRAVRALVERMGDERLDAAMIRSM
                     AASQVGA"
     gene            4071..>5349
                     /locus_tag="VNN25_15685"
     CDS             4071..>5349
                     /locus_tag="VNN25_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HXH93023.1"
                     /translation="MTMLRSVPMVHFRAQVPSRDAAVATRAIAGEGLLHLVDIAHGHA
                     PYDASPPGVRELYAGFRDVVHRIRATADRLGVPRAEATGALDGDDLADFAVERERTLL
                     ILEPIEKRVQDLVRANAEASERLTAAREWCDDAERLRLASLDIDRLAALRFAAIRLGM
                     QSAEALPAMAEMLAPSAHAVVSLHEREGSVLFAAAVPAYNRARLDESMRLASARAVTL
                     PKSAADLDAERLARELAGAERVVADAQRAFEEERTRSSEVLDRIARRAETAMLLLQAQ
                     TFFAAAGRFIVISGWVPCDSAEPLAARVRTATKGHAIVEIEPVASVPGVSEGTLRVPI
                     LYRNPLLLRPFQKLIDIYGTPSYEEVQPTAFFAVGFLAMFGLMFGDAGHGAVLFLAGW
                     FLFRYLPRFLDYGILLMEAGVASAVFGLLYGSLF"
BASE COUNT          940 a         1746 c         1720 g          943 t
ORIGIN      
        1 atccgccggc cgcagcaatg cgccggtatg tcgcaagcca gcggcgaagt gcggcgtact
       61 ccggatgtgc gggcggcatg cgcgcgatcg cagcgcgcac gtcgtgcagc tcgagcgcgg
      121 tctccaacgc agcgacgaga tcgttcgccc gcggcgtcag acaccaggtc ggctcgatcg
      181 attccggatc gacgcggccc gaaagcaagt gcgaggcgag cagaaagaat gcgtctgtga
      241 gcagaaggtc gcgctgttcg ttatcagacg ctcgttcgat ggcagccaga tggtaatcag
      301 ccggatcgag gccgtcgctc ttggcatcgc gaatggcggc cagcagctcg ccgcgatcgc
      361 gcgcgctcca cgccggtgca aactgccggc ggctgtagaa aagcggaaga ggacgcgacg
      421 cacagatggc atcgccttcg atcatcacga ttttcccgtg gctcagcgag tcgatcgcag
      481 atcggatcgt gaccgtatcg ccggctgtca cgtcagtaac cgcgacggca agactcagcg
      541 caaatgcgag tgcgattcgg agcacgtaac gatcgtcctc cttcctcgat cgagccgccg
      601 ccatcaagcg cacgtgacgg cgtgaccacg gtcactcgcc gcgcatcacg atccttacga
      661 accggtgacg cacaccatcg gtgcggggct gcggccgtca cctccacatg tgctccatgc
      721 cgcggtgctc aagcgtgcgg cgacgcatct tctttccatg atgaaagtcc gcgatgccat
      781 gactcccgat ccggccacgt gcgatgcggc gacgacgctc cgtctcgtag ccatgctgat
      841 gctcgatcac gactgcgccg ctgttcccat cgtcagcggc gatgaaatcg tcgggatcgt
      901 taccgaccgc gacatcgcat gccgcgcggt cgcccgaggg tggaacgcgg cggaccttcc
      961 cgcgtcggcc gtcatgacgt cgccgctcgt cgccatcaac cccgacgaga cgttcgaaga
     1021 cgcgattcag gtaatgcggg aaaaccacgt ccaccatctc ccggtcatcg acgacgacgg
     1081 acgcctcgtc ggcatcgtcg cgcaatccga cctcgggcgg cggatgacga accgcgagct
     1141 cggactgctc gcgcgggaga catcgattcg aagagcgggg cgggggccgg cgaagctcgt
     1201 gcgcgcgcgc gcgtagcgaa ctgactgagg aggtcttctt atgagatcag cgagttcccg
     1261 atttctcaga gcggctggag ccatcgcaac cggtgccacc gcgctggcgt acaccgcgta
     1321 tcgcaaggat atcgaagcag ccaggcggcg aatcgacttg gaccggcaga tcatcgatac
     1381 cgagcaaggg cggatcgagt ttgcggagtc tggcgacgga ccggcagtcc tgctcatcca
     1441 cggcgccggc ggcggcttcg atcagggcct cgacctcggg cgcgcctttc tcggcgacca
     1501 ctaccgcatc gtcgccccgt cgcgcttcgg ttacctcggc actccacttc cggccgacgc
     1561 ttcagccgaa gcccaggcgg aggcgcacct gcgactcttt gatgccctgc agttagaccg
     1621 cgtcccagtg atcggcgttt ccgccggcgg tccgtctgcc atgcagtttt gcctgcgcca
     1681 tcccgagcgc tgctcggcgc tgctgctgat tgtgccgttg gcgtacgcac cgcagcgcgc
     1741 cgccgttcgc gttctgtcgc ccattttcga gaccgtgttg aacacgatcg cggcatcaga
     1801 ttttctcttc tggaccgcga cgaaagtcgc gcggatgact ctcctcaaga ccattctcgg
     1861 cacgccggtg gatgcttacc ggaacgcaac gccggacgag cgccgaagcg tcgatcagat
     1921 gctgcgcggc attcttccca tcagcagacg cgtcgcagga atctggaacg acagcgtcgt
     1981 ttcaaccacc ctgacgcgat atcagctcga agacattcgc gttccctcgc tggtgatcag
     2041 cgcggcggac gatctgtacg gcacctacga aggcggcctc tacaccgccg agcagatcca
     2101 caacggaaca ttcatcggct tcggatccgg cggccatctc ctcctcggcc accaagcgga
     2161 agtgcgttcg cagatcacga actttctgga acgactcgaa acctccggag gaacagcgat
     2221 ggccatctga tcgctcgagc ggtcatcgag gcgaatggta tcgttatgaa cgccggcagg
     2281 tgacgcggcg ctccgcttgg cgaaagaaac atcacagaca gcagccgcgg aagggcgcac
     2341 atttaagaga tgaagcacta tcgtcttgcc gttctccttc tcgccgtgtt gcccctttcc
     2401 gcggctgccg cgccgcgcgc gattccgggc atcaacgcta aagacgtcta ccccgccggc
     2461 tgcatcgact gccacacgaa gaaggccggc ggcgcggaca ctcgcatcag tgttcttctt
     2521 gcccgctgga acgggagcgt cgatccgaag agactcgccg ccatgcagac gtttgttccg
     2581 aaaggaatga cgctgaaggg gaaacatccg gcggtgacgg cggcggtgaa ggacgttcct
     2641 tcctcatgcg tgaaatgcca cgccgccggc tcgaagacca tgccgccgct cgcaccactg
     2701 atgcacggag tgcatctggc ggaaggtgac caaagtgagt tcgtaaagca gttccagggc
     2761 gaatgcacgt actgccacaa gctgaacaaa acgtccggaa cgtggttgat gccgaacggc
     2821 gctgagaagt agcggcgatc acaccgatcg gtgagcggcg gcatcgcgcg gcaagtgctc
     2881 ctgctgcact gttacggcgg aggcatggat gcatcccgac ctcgaggcga tcgtgtcggc
     2941 ggacgaggaa gcgcgctctc gcgtgtcgct tgccgggaag cggcgcgagc atgacacgct
     3001 cgcggctcgt acggcacgcg atgccacgat cgatgcgcat ctgaagaaag aatccgacgc
     3061 tctcgatcgg gagctcgacg gcattcgcgc ggaaggcgat gcacgcatcg gcgaattgaa
     3121 aaaacagcag gatcagtact tggcatcact cgccgcggcc ggagaacgga agttcgatga
     3181 tgctgtcgca ctcgtccttc gtatggtctg cgaggtggcg tgatgagagc gcgaacaccg
     3241 tctcgcggct cacttgcgta cgcgcacgcg cgcattcgcg cctggaagtc ccgcctgctg
     3301 acacgcgccg gcgcggcacc tctcttcgcc gcaaccgacg ccgccgccat ccagcgcgcg
     3361 atcgccgcat tagaactcga cgatccgttg cgtcgtctgt tgcgggttta tcagaccgct
     3421 atcgatggat acagcaacgg cgccccactc ttccgcgcga tgcttcaacg gcacgaaatc
     3481 gagaacgtca agctgctctg gcgcgtcgtc gcgaacggac atcgcgatcg ggtcgcgcgc
     3541 ctctggatcg acctcggcgc ctttgcatcg tttcctcttc ccgaagcccc gacgctgaac
     3601 gaattggtgg cacaggtcgc ggcgacgccc tacgcggaga ttgcgaggag tgtcgcacgt
     3661 gtacacggga ccgacctcgc cgccgcggag cttgcgttcg accgctgggc gtcgcggcgg
     3721 ttgctcgaag aagcgcgcag ccttccgaaa caggaatcga tcgcgcgccg gctcatcgag
     3781 ctcgtcgtcc gcgaacgcga agcgcagctc gttctccgcg gcgcgaagtg gtatggactg
     3841 gcaagcgtcg cgccgggcaa catcacggac atcaccgctc tccgtcgcga gcggctgcgc
     3901 ttctgccacc gcgcattcat cggcaggcca tttcttctcg gttctgcagt cgccgtcatt
     3961 cttctcgccg aggaggaagt gcgagcggtt agggcgctcg ttgagcggat gggcgatgag
     4021 cgcctcgatg cggcgatgat ccgctcgatg gcggcatcgc aggtcggagc atgaccatgc
     4081 tgcgctcggt gccgatggtt cacttccgcg cgcaggtgcc gagccgcgac gcggctgttg
     4141 ccacgcgtgc catcgcaggc gaggggctgc ttcatctcgt cgacatcgcg cacggccacg
     4201 cgccgtacga cgcgtctcct cccggcgtgc gcgagctcta cgccggattc cgcgacgtcg
     4261 ttcaccgcat ccgcgcgacc gccgatcgtc tcggcgttcc gcgcgcagaa gcgaccggtg
     4321 cgctcgacgg tgacgatctc gccgactttg cggtcgagcg ggagcgtacc cttctgattc
     4381 tcgagccaat tgagaagcgc gtgcaggatc tcgtacgcgc gaacgcggaa gccagcgagc
     4441 ggctgaccgc ggcgcgcgaa tggtgcgacg acgcggaacg ccttcgcctg gcatcgctcg
     4501 acatcgaccg gctggctgcc ctccgtttcg ccgcgatacg actcggcatg caatccgcgg
     4561 aggcgctgcc cgcgatggcg gaaatgctcg cgccatccgc gcacgccgtg gtctctcttc
     4621 acgagcgcga aggatcggtg ctgttcgccg ccgccgtgcc cgcatacaac cgggcgcgcc
     4681 tcgacgaatc gatgcgcctc gccagcgcca gagcggtgac gcttccgaag tcggcagccg
     4741 atctcgatgc tgaacggctt gcgcgcgagc tggccggggc ggaacgcgtc gttgcggatg
     4801 cgcaacgcgc attcgaggag gagcgcacgc ggtcgagcga agtactggac cggattgccc
     4861 ggcgcgccga gacggccatg ctcctcctgc aggcgcagac cttctttgcg gcggccggcc
     4921 gtttcatcgt tatctcggga tgggtaccgt gcgacagcgc ggagccgctg gcagcgcgcg
     4981 tccgcacggc tacgaaaggg catgccatcg ttgagatcga gccggtcgcg agtgtgccgg
     5041 gcgtgagcga aggaacgctg cgcgttccca tcctctatcg caatccgctg cttctgcggc
     5101 cgtttcagaa gttgatcgac atctacggca cgccgtcgta cgaggaagtg cagccgaccg
     5161 cattcttcgc cgtgggattc ctcgccatgt tcggactgat gttcggggac gccgggcacg
     5221 gtgcggtgct ctttctggcg gggtggtttc tctttcgata cctgccgagg tttctcgact
     5281 acggcattct gctgatggag gccggcgtcg cgtcggcggt cttcgggttg ctgtacggga
     5341 gcctgttcg
//
LOCUS       DATLVW010000526         9917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_357037, whole genome shotgun sequence.
ACCESSION   DATLVW010000526 DATLVW010000000
VERSION     DATLVW010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357037"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNN25_15690"
     CDS             complement(<1..283)
                     /locus_tag="VNN25_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HXH93024.1"
                     /translation="MGTLRKWFGPSREEVWRQLSDSLHGRFIEGGFFKSDRVEAEHGL
                     WTVTLDTYTVSTGKATIIFTRMRAPYVNPDGFRFTIYRKGFFSEIAKWFA"
     gene            complement(365..841)
                     /locus_tag="VNN25_15695"
     CDS             complement(365..841)
                     /locus_tag="VNN25_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93025.1"
                     /translation="MNKPDCVREIETFDAISSGAWPDACDDSLRAHVATCTICSDVVE
                     VATALIDDRDAALRAAPVPPSGVVWWRMQMRARNEATQSARRTVVAVQAAVFAAVLVI
                     ALAILGGAWWNKLPHIDEIAASGLLAAAQWGVPLLLAIAIWLTLAPVAVWLAVTKD"
     gene            complement(834..1427)
                     /locus_tag="VNN25_15700"
     CDS             complement(834..1427)
                     /locus_tag="VNN25_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH93026.1"
                     /translation="MPFSVDMTLSELGLRSRVEEAATAAPMDEDAFRAFYERTARPVW
                     AYLRRITGDAQLADDLLQEAYYRFFRASADHHDETHRRNYLYRIATNLARDQVRKHRH
                     GYDVALPGEGDPDQLRDDGTTAHRAHIRMDLGRAMSQLGAMQRQMLWLAYAQGSSHDE
                     IAQVVGVNTGSVKSLLYRARRKLAELLRGHDDGGKRE"
     gene            complement(1455..2363)
                     /locus_tag="VNN25_15705"
     CDS             complement(1455..2363)
                     /locus_tag="VNN25_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93027.1"
                     /translation="MTTNRLRLAIGIILLALTPLMMAAPATTPATSTATTSTAATDTS
                     TATTATPARIDDYDSHETREKLRELLHTYPPAVGMVLRLDPSLFNNTAYLASYPALAS
                     FVAAHPEIAHAPNFYLQTVSVAYPSDTRQDVQHERTIRILAGLAGLFVFLTVTITLAW
                     LVKTLIQQRRWNRQSHVQTEVHSKLMEKLTTGEEMIAYAQSPAGKRFLEGGPIALDAA
                     AQPVSLPVSRFLWSLQAGLVLSAAGFGMEIVSGQMPIDVTPVASGVGILVLSIGIGFV
                     VSAIVSFVLSRKLGLVPANPPAGDAA"
     gene            2728..3507
                     /locus_tag="VNN25_15710"
     CDS             2728..3507
                     /locus_tag="VNN25_15710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXH93028.1"
                     /translation="MTGGVWAKAHLASSPRHREWVNVKAGNRVVSCFVVYPEVKNKAT
                     AVILIHEIFGLSDWVQMEADEVAAEGYIAIAPDLLSGMGPKGGGTSDIGDVNAAVKVI
                     GTLPPDQITSDLNAVADYVTKLPSANGKLAVAGFCWGGTQSFRFATNRPGLSAAFVFY
                     GTGPDAESIARIKAPVYGFYAGNDARVGATIPATTELMKAAGKTYEPVTYEGAGHGFM
                     RAGDAPPPPATAEAKARDAYAGNKKARDAAWVRWTALLKKL"
     gene            4107..5333
                     /locus_tag="VNN25_15715"
     CDS             4107..5333
                     /locus_tag="VNN25_15715"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXH93029.1"
                     /translation="MEVVVVVGAGLDVHKKKIVACCIDGRNSPPTVVTCTFGTFRDEI
                     ERMRDWLIQRECTHVAMESTGVYWMSVYEVLEGVLEIILGNPRHMANVPGRKTDVSDA
                     HWIAKLLRHGLIQPNFVPPRPIRDLRQATRYRRKLMQTRTACLLRVEKLLQCATIKLS
                     SVASNIFGVSGRLMLAGLAGGVTDPQELSEMAKGKLRGKRGALKRAFRGSFREEDASL
                     LQVELQVIADLEAQLEQLGALIEQKVGSFEDVIALLDTIPGVNRVTAIDLIAEIGTDM
                     TAWKTDRQFAAWSGTCPGNRESAGVRKKAKAREGNPYVKTILIQAAVSASQTIGSHLA
                     HRFHRLAARRGPRRAFVAVAREIAVALYHMISEKVPYTPPRNADPEVTRQQRSRHLMR
                     ELTKLGYEVNCKPLKT"
     gene            complement(5506..7770)
                     /locus_tag="VNN25_15720"
     CDS             complement(5506..7770)
                     /locus_tag="VNN25_15720"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HXH93030.1"
                     /translation="MKTTRRLLWLTVLLCLSIAVHAQTREPATTFAIKRASGPITIDG
                     DLSDAAWKDAVRVDKWYETNATDNTEPKVKNTAWMTYDDHFFYAAFHFEDPNPAAIRS
                     PFADRDNIPSSTDYAGVILDTRNDGKTAILFLSNARGIQYDSVSDDTSGNEDSSPDFF
                     WESAAKITKEGWDLELRIPFSSLRYKKADPQTWGILLYRNYPRDRRYQFFANKIPKGG
                     NCFICNENKITGLAGLPQGGHVVAAPYMTVKEQGTPRGDVLGNRLVNAPAKVDGGIDV
                     KWTPNADTAIDGTLNPDFSQVESDVAAISANQRFAIFFSEKRPFFLEGIELFNTPIQA
                     VYTRTITSPRFGLRSTGKFGDNDYTAIIAQDRGGGLTILPGPNGSDFANQDFNSTVAI
                     GRIRHNFGLSYLSLLGTFRESEGSAHNRVLGPDFQWRINDKETVTGQVLFSDTKTPDR
                     PDLAAEWNGQTLKGHAADVWWSHSTKHVDWYTEAKDYSDGFRADNGFVPQVGYREEFF
                     EGGYTTRPTTGFFNRVRVFLNGDYSAATNGDMLFRDRSAGVGFDGKFASSTRLRFSIN
                     GVRSGDKVLTRHQLLYSTQFTPSRVFQNIQINGSAGEDVDFSNTRLGRGADISVGGIV
                     RPTDHLEFRYNQGLRWLNVNPLPGQVSAERARLFTAQVERIRATYTFNSRMYVRGIVQ
                     NVRTNRNQSLFLNSIDQHSGNVTSSLLFAYKVNWQTLLFVGLGDEHDVDVADNKLQPA
                     SRQLFMKMSYAFQR"
     gene            7922..9205
                     /locus_tag="VNN25_15725"
     CDS             7922..9205
                     /locus_tag="VNN25_15725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH93031.1"
                     /translation="MRKLFLLLAALFAVSTFAQQPKTIVLKAARIFDGTSDAVTANGV
                     IVIEGNHIKSIGGAVPANAQVIDLGDATLLPGLIDSHVHLTSEIGDNFYVDYFQNLMR
                     HPAEQAILATTYARKTIDAGFTTVRNLGASDYVDVGLRNAINHGWAVGPRMLVAVHAI
                     GATAGHGDSDPIPPTAGIPPLGPMQGVCNGAAECRAAVRYQIKYGADVIKFMPSGGVL
                     SLSDPVDAPELSQDEMNAIVEEAHHWERKVAAHCHGDSAARMAITAGVDSIEHGSFIK
                     PETLTMMRDKGVYLVPTLLAGEWTGGKADKFPPAIATKARAALAARSEMFRNALKIGV
                     KIAFGTDSAVSPHGMNGKEFALMTGLGMSPAAALRSATSVAAGLLGVPDRGTLATGKL
                     ADIVAVPGNPLTDITAMERVSFVMKDGVVVKGAAR"
     gene            9290..9613
                     /locus_tag="VNN25_15730"
     CDS             9290..9613
                     /locus_tag="VNN25_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93032.1"
                     /translation="MGVELNKTFVEANKLFDGANKSFDELNKLFDGVNKTFDELNKSF
                     DGVNKTFDGANKTFDGVNKLLDGVNKLFVELNKLFDGANKTFVAVNKFFVELNKFLLT
                     PTKFC"
BASE COUNT         1940 a         2987 c         3120 g         1870 t
ORIGIN      
        1 aggcgaacca tttggcgatc tcgctgaaga agcccttcct gtagatcgtg aagcggaagc
       61 cgtcgggatt cacgtacgga gcgcgcatcc gcgtgaagat gatcgtcgct ttgccggtcg
      121 atacggtgta cgtgtcgagc gtaacggtcc acaggccatg ctcggcctcc actcggtcgc
      181 tcttgaagaa gccgccctcg atgaaacggc catggagcga gtcgctcaac tgacgccaga
      241 cctcttccct gctgggacca aaccatttgc gtaatgtgcc catgggaaca tcgtaacgct
      301 gaagtcgcag gcgccgcctg cggtacagcc gccgagccgg cggcgctacg ccgcgaaagg
      361 aacgtcagtc cttggtgacg gctagccaga cggcgacggg tgccagcgtc agccaaattg
      421 cgatggccag cagcagcggg acgccccatt gcgcggcggc gagcaggccg gacgcggcaa
      481 tctcgtcgat gtgcggaagc ttgttccacc aggcgccgcc gaggatggcg agggcgatga
      541 ccaggactgc ggcaaagacg gcggcctgca cggcgacgac ggtgcggcgc gctgattgcg
      601 tggcttcgtt gcgcgctcgc atctgcatgc gccaccagac cacaccggac ggcggtacgg
      661 gagcggcgcg cagtgcggcg tcgcggtcat cgatcagcgc cgtggccact tcgaccacat
      721 ccgaacagat cgtgcaggtg gcaacgtggg cgcgcagcga atcgtcgcag gcatccggcc
      781 acgcaccgct gctgatcgca tcgaacgtct cgatctcgcg aacgcagtcg ggcttattca
      841 cgtttccctc cgtcatcatg tccgcgcaga agctcggcga gcttgcggcg ggcgcggtag
      901 agcagcgatt tgacggagcc ggtgttcacg ccgacgacct gtgcgatctc gtcgtgcgaa
      961 gaaccttgcg cgtaggcgag ccagagcatc tgccgctgca tggcgccgag ctgcgacatg
     1021 gcgcgcccga ggtccatgcg gatgtgggcg cggtgcgcgg tggtcccgtc gtcgcggagt
     1081 tgatcgggat cgccttcgcc ggggagcgcg acatcgtagc cgtgacgatg tttgcgcacc
     1141 tggtcgcggg cgagattcgt ggcgatccgg tagaggtagt ttcgtcgatg cgtctcgtcg
     1201 tgatgatcgg ccgaggcgcg gaagaaacgg tagtaggcct cctgcaacag atcatcggca
     1261 agttgggcat cgccggtgat ccgccgcagg taggcccaga ccggccgcgc ggtccgttcg
     1321 tagaaagccc ggaacgcatc ttcatccatc ggcgctgctg ttgccgcttc ctcgacgcgc
     1381 gagcggagcc cgagctcgga caaggtcatg tcgacgctga atggcatctc attaatgata
     1441 accgtaggtt cccgctacgc ggcgtcgcct gccggagggt tcgctggaac aagtcccagc
     1501 tttcgcgaca gcacgaacga aacgatcgcc gagaccacga atccgatccc gatcgagagc
     1561 acgagaatgc cgactccgga cgcgaccggc gtgacatcga tcggcatctg accgctgacg
     1621 atctccatac caaaaccggc cgccgaaagg acgaggccag cctgcaacga ccagaggaag
     1681 cggctgaccg gcaggctgac cggttgggca gctgcgtcga gtgcgatcgg accgccttcg
     1741 aggaatcgct tgccggcagg cgattgggcg taggcgatca tctcctcccc tgtcgtcagc
     1801 ttttccatca gcttgctgtg cacctcggtc tgcacatgcg attggcggtt ccagcggcgc
     1861 tgctggatga gcgtcttcac cagccaggcg agcgtgatgg tgacggtgag aaacacgaac
     1921 aggccggcca gaccagcgag aatcctgatg gttcgctcat gctgcacgtc ctgccgcgta
     1981 tcggagggat aggcgacgga gacggtttgc agataaaagt tgggagcgtg cgcaatctcg
     2041 ggatgcgcgg cgacaaaaga agccaacgcc ggatacgacg ccagatacgc tgtgttgttg
     2101 aagagcgatg ggtcgagcct cagcaccatc cccacggccg gcgggtaggt atgaagcagc
     2161 tcgcgtaact tctcgcgcgt ctcatggctg tcgtaatcgt cgatgcgggc gggcgtcgca
     2221 gtcgtggcgg tgctggtatc ggtggcggcg gtgctggtgg tggcagtgct cgtcgccggt
     2281 gtggttgccg gagcagccat catgagtggc gtgagtgcca gcaggatgat cccgatggcg
     2341 agtcgtaggc gattcgtggt catggttttc gtcctcatga cccggtatta cgttgaaatt
     2401 ggcattcggt gacgggggga ggatcagaga tcagagatag cagagatcag agatcaggaa
     2461 ggcggccagt ttcggatctc ctgatcccct gatccactga tgctctgatc tctagtctct
     2521 gatctctagc ctctggtgtc tctgatctct ggtctcttga tccgatagaa tctgccgagt
     2581 tcacaaccgg aggaaacgat gaagaaatcg atgctcgcga tcgccatgct ctcgctcgcc
     2641 gtcaccgtgg cctgcgctca ggaaacatcg accgctcatg ccgaccatgc cgcggccgcg
     2701 gcagcagcac cgcccggacc gtccgaaatg accggaggag tctgggccaa ggctcacctc
     2761 gcgtcatcgc cgcgtcatcg cgagtgggtg aatgtgaagg cgggcaatcg ggtcgtcagt
     2821 tgcttcgtcg tctatcccga ggtgaagaac aaggcgacgg cggtgatctt gattcacgag
     2881 atcttcggct tgagtgactg ggtgcagatg gaagccgacg aagttgccgc cgagggatac
     2941 atcgccatcg cgccggacct gctgtcaggc atgggcccga aaggcggcgg cacgagcgac
     3001 atcggcgacg tgaacgcggc cgtcaaggtc atcggcacct tgccccccga tcagatcacc
     3061 tccgatctga acgccgttgc tgactacgtc acgaagctcc cctcggccaa cggaaagctt
     3121 gcggtggccg gattctgctg gggtggtaca cagtcgttcc gcttcgccac gaaccggccc
     3181 ggcctttccg cggccttcgt gttctacggc accggccctg acgccgagtc catcgccaga
     3241 atcaaagcgc cggtctacgg cttctatgcc ggcaacgacg cacgcgtcgg agcgacgatt
     3301 ccggcgacga ccgagttgat gaaggcagcc ggcaagacgt acgagccggt cacgtacgaa
     3361 ggcgcggggc acggcttcat gcgcgccggc gacgcaccgc cgccgcctgc caccgccgag
     3421 gcgaaggccc gcgatgcgta cgccgggaac aagaaggccc gcgacgcggc ctgggtgcgt
     3481 tggacggcgc tgctgaagaa gctgtaatcg agctgttcat gtgacgccgg ttttgggtgc
     3541 aggaagacgg taaatggcgt cgtacaagta acaccaatcc tcgagggggg cctagcccgc
     3601 atttcgcaca catttctgcg acctccttcc catcgagcgc cttctccccc cgcgcttttt
     3661 gcggggggag aaggtggcga agccggatga ggggggcgtc gaaagatggg acaaccacgt
     3721 tctctgcgag cgtgacctga ttgaaagggc atccttgtcg gggtttgcgc aacgttggcg
     3781 cgacaggcaa acaccccctc atccggcctt cggccacctt ctcccccact gaaaagcgtg
     3841 ggggagggac cgacgtcctg cggccgaccg ccgtggacga agttgcatgc gggaccagcc
     3901 ggaccgacct ttagcggtgt ctaagacgcg aagaggtcga aaccttaact gaggctaccg
     3961 agccccgtgg gggaggctga tcccgcgccg acataggtac caccgtttga tgacgttcgg
     4021 aagtgtttcg aacaagcacg tagagcagat tctgagttcg aatggcggcg tacgcgttct
     4081 tggacacctc atgaaaggag ggtgtcatgg aagtagtcgt tgtagtggga gcaggtctgg
     4141 atgtgcacaa aaagaaaatc gtggcgtgtt gtatcgatgg gcggaacagc ccgccgacgg
     4201 tggtcacgtg cacattcgga acgtttcgcg atgagatcga gcgcatgcgt gattggttga
     4261 tccaacggga atgtacgcat gtggcaatgg aaagcaccgg cgtgtactgg atgtcggtat
     4321 acgaggtgct tgagggagta ctggagatca tccttgggaa cccccggcac atggcgaatg
     4381 tccctgggcg gaagacggat gtttccgatg cgcattggat cgccaagctt ctgcggcatg
     4441 gcctgattca accaaacttt gttccaccgc ggccgattcg tgatctgagg caggccacgc
     4501 ggtatcgacg caagctgatg caaacgcgca cggcatgtct gctgcgcgtg gaaaagcttc
     4561 tgcagtgcgc gaccatcaag ctgtcttcgg tggcttccaa cattttcggg gtctctggac
     4621 gtctgatgct ggctggattg gctggcggag tgacggatcc ccaggagctg tcggagatgg
     4681 ccaaaggaaa gctccgaggg aagcgagggg cgctgaagcg cgcttttcga ggatcgttcc
     4741 gagaggaaga tgcgagcctg ctgcaagtgg agctgcaggt catcgccgat ctggaagctc
     4801 aactcgagca actgggagcg ctcattgagc aaaaagtcgg ttccttcgaa gacgtgatcg
     4861 ccttgctcga caccattccc ggcgtgaacc gggtaacggc aatcgatctg atcgctgaga
     4921 tcggaacgga tatgacggca tggaagaccg atcgtcagtt cgcagcctgg tcaggaacct
     4981 gtcccggcaa tcgcgaaagt gccggcgttc gcaaaaaggc caaggctcgc gaaggtaacc
     5041 cttacgtgaa gaccatcctc attcaggccg cggtgagcgc gtcacagacc attggttccc
     5101 acctcgcgca ccgcttccac cgtcttgctg cgcgccgcgg acctcgacgc gccttcgtgg
     5161 ccgtcgctcg cgaaatcgcc gtcgcgctct accacatgat ctctgagaag gtcccgtaca
     5221 caccaccccg gaatgctgat cccgaggtca cccgacaaca acgctcgcgc catctcatgc
     5281 gtgagctcac caagcttggt tacgaggtca actgtaagcc gctgaagacc taacctttct
     5341 cccgcgccgc gcccaacggc cgacagaacc accctttcga acggcgtctt gagccgtaca
     5401 ggattccttt gtctccccac ccaccctaac ttcagagcag aaggcgctcg attgggagca
     5461 cagtgccagc aattccaatg caagtgtgag aaatgcggcg tctccctacc tctgaaacgc
     5521 gtacgacatc ttcatgaaca actgccggct ggctggctgg agcttgttgt cggcgacgtc
     5581 gacgtcgtgc tcgtcgccga ggccgacgaa gaggagcgtc tgccagttca ccttgtaggc
     5641 gaagagcagc gatgacgtca cgtttccgct gtgctgatcg atcgagttga ggaagagact
     5701 ctggttgcgg ttcgtccgga cgttctgcac gatgccgcgc acgtacatcc tcgagttgaa
     5761 cgtgtaggtc gcacggatgc gctcgacttg cgcggtgaag aggcgggcgc gctcggcact
     5821 gacctggccc ggcaacggat tgacgttcag ccagcgcagg ccctggttgt agcggaactc
     5881 cagatgatcg gtaggacgga cgatgccgcc gacggaaatg tcggctccgc gcccgaggcg
     5941 cgtgttcgag aagtcgacgt cctcgccggc tgagccgttg atctggatgt tctggaagac
     6001 gcgcgacggc gtgaactgcg tcgaatacag aagctggtgg cgcgtgagca ctttgtcgcc
     6061 gctgcggacg ccgttgatcg agaagcgcag tctcgtgctc gaggcaaact tgccgtcgaa
     6121 gccgacgccc gcggagcggt cgcggaagag catgtcgccg ttcgtggctg ccgaatagtc
     6181 accgttgagg aaaacgcgga ctcgattgaa gaagccggtg gtcgggcgtg tcgtgtagcc
     6241 cccttcgaag aactcctcgc gatagccgac ctgcggcacg aagccgttgt ccgcgcggaa
     6301 cccgtcgctg taatccttcg cctcggtgta ccaatcgacg tgcttcgtcg aatgcgacca
     6361 ccagacgtct gcggcgtgtc ccttcagcgt ctggccgttc cattccgcgg ccaggtcggg
     6421 gcggtccggc gtcttcgtgt cgctgaaaag gacctggccc gtgaccgtct ctttgtcgtt
     6481 gatccgccat tgaaaatcgg gcccgaggac gcgattgtgc gcgcttccct ccgactcgcg
     6541 gaacgtgccg aggaggctga ggtaggagag gccgaagttg tgccggatgc ggccgatcgc
     6601 aaccgtcgaa ttgaaatcct gattggcgaa atcggaaccg ttcggccccg gcaggatcgt
     6661 caatccaccg ccacggtcct gggcgatgat cgccgtgtag tcgttgtcgc cgaacttgcc
     6721 ggtcgaccgc aggccgaacc gcggcgacgt gatcgtgcgc gtgtaaacgg cctgaatcgg
     6781 cgtattaaac aactcgatgc cttcgaggaa aaacggccgc ttctcggaga agaaaatcgc
     6841 gaagcgctgg ttcgccgaga tcgccgcgac atcggactcc acctgcgaga aatcagggtt
     6901 gagcgtgcca tcgatggccg tgtccgcgtt cggcgtccac ttcacgtcga taccgccatc
     6961 gaccttcgcc ggagcgttga ccagccggtt tccgagtacg tcgccacgcg gcgtgccctg
     7021 ctccttgacc gtcatgtacg gcgcggcgac gacgtgcccg ccctgcggca gcccggccag
     7081 accagtgatc ttgttctcat tgcagatgaa acagttgccg cccttcggaa tcttgttcgc
     7141 gaagaactgg tagcggcggt cgcgcgggta gttgcggtag aggagaatgc cccaggtctg
     7201 cggatcggct ttcttgtagc gcagcgatga gaacgggatg cgaagctcga ggtcccatcc
     7261 ctccttcgtg atcttcgcag ccgactccca gaagaagtcg ggggagctgt cttcgttgcc
     7321 gctcgtgtcg tcgctcaccg agtcgtactg gatgccgcga gcgttcgaca gaaagagaat
     7381 ggccgtcttc ccgtcgttgc gcgtatcgag gatgacgccc gcgtagtcgg tcgagctggg
     7441 gatgttgtcg cggtccgcga acggcgagcg gatcgcagca ggattcggat cttcgaaatg
     7501 aaaggcggcg tagaagaagt gatcgtcgta cgtcatccag gccgtgttct tcaccttcgg
     7561 ctcggtgttg tcggtggcgt tcgtctcgta ccacttgtcg acgcgcaccg cgtccttcca
     7621 ggcagcatcg gagagatcgc cgtcgatggt gatcggacca gacgcgcgct tgatggcgaa
     7681 cgtcgtcgcc ggttcgcgcg tctgggcgtg cacagcgatg gaaagacaaa gcaatactgt
     7741 gagccagagc agccgccgag tcgtcttcat tgcaccgtca gacgtagagc ggctgggaaa
     7801 gttagggcgg cagacccaat tctccgcaag gtttgcgatc cgtgaccgga acgtgaactc
     7861 cccgcgtccc ccgcgcctcc gcgtgaaacg cctctaaaaa ccatgctagc ttccactcat
     7921 gatgagaaag ctcttcctcc ttctcgccgc tctcttcgcc gtttcgacct tcgcgcagca
     7981 gccgaaaacg atcgtcctga aagccgcccg gatcttcgac ggcacctcgg acgccgtaac
     8041 cgcgaacggc gtcatcgtca tcgaagggaa tcacatcaag tcgatcggcg gcgcggtgcc
     8101 cgcgaacgcc caggtcatcg acctcggcga cgccacgctt ctgcccggcc tgatcgactc
     8161 gcacgttcat ctcaccagcg agatcggcga caacttctac gtcgattact ttcagaacct
     8221 gatgcgccat cccgccgagc aggccattct ggccacgacg tatgcacgaa agacgatcga
     8281 tgccggattc acgaccgtcc gcaacctcgg cgcgagtgac tacgtcgacg tcggtctgcg
     8341 caacgccatc aaccacggct gggcggtcgg accgcgcatg ctcgtggccg tgcacgcgat
     8401 cggcgcgact gcaggacacg gcgacagcga tccgatcccg ccaacggccg gcattccgcc
     8461 actgggcccg atgcagggtg tctgcaacgg tgccgcggag tgccgggcgg ccgtccgtta
     8521 tcagatcaag tacggcgccg acgtcatcaa attcatgccc tcgggcggcg tgctctcgct
     8581 ctccgaccct gtcgacgcgc ccgagctgtc gcaggacgag atgaacgcga tcgtcgagga
     8641 agcgcaccac tgggagcgca aagtggccgc gcattgtcac ggcgattccg ccgcgcgcat
     8701 ggccatcacg gccggcgtcg actcgatcga gcacggctca ttcatcaaac ccgagacgct
     8761 cacgatgatg cgcgacaaag gcgtctacct cgtccccacc ctgctcgccg gcgagtggac
     8821 gggcggcaaa gccgacaagt tcccccccgc tatcgccacc aaagcgaggg cagcactggc
     8881 cgcccgcagc gagatgttcc gcaacgcgct gaagatcggc gtgaagatcg cgttcggcac
     8941 cgactccgcc gtctcccctc atggcatgaa cggcaaagaa ttcgcgctca tgaccggact
     9001 gggcatgtcc cccgccgccg ccctccgctc cgccacatcc gtcgccgctg ggctcctcgg
     9061 cgtccccgac cgcggcaccc tcgccaccgg caaactcgcc gacatcgtcg ccgtccccgg
     9121 caaccccctg accgacatca cggcgatgga gcgggtgtcg ttcgtgatga aggacggggt
     9181 cgttgtgaag ggggcggcca gataggttca cgcgccggcc aacaagttcg gagttggcgt
     9241 caacaagttc cgagttggcg ccaacaaaac ttttgttgcc gccaacaagt tgggagttga
     9301 gctcaacaaa acttttgttg aggccaacaa actttttgat ggcgccaaca aatcttttga
     9361 tgagctcaac aaactctttg atggcgtcaa caaaactttt gatgagctca acaaatcttt
     9421 tgatggcgtc aacaaaactt ttgatggagc caacaaaact tttgatggcg tcaacaaact
     9481 tcttgatggc gtcaacaaac tttttgttga gctcaacaag ttgtttgatg gcgccaacaa
     9541 aacttttgtt gcggtcaaca agttttttgt tgagctcaac aaatttttgt tgacgccaac
     9601 aaaattttgt tgacaccatg gttgcttggt ttcgtaaagc aactgatttt ggaacaaaag
     9661 cggttaaaac gcgagggcag gcgcgggcag cgtgctcatt tattcccagc ggcgaggctg
     9721 tgtcatcctg agccgccgaa gccgccgcga gatttcgtac gctcgctacc ggcagacatc
     9781 ggcggcgcag gacggtcgaa ggatctcaaa cttcgcgaat catgttcgtc cagcgcgagc
     9841 gatcgtgatc aaagaccgct cgaacacgat cttcgcattt tgagatcctt cgaccgtcct
     9901 ccgccgcaat gcctgcc
//
LOCUS       DATLVW010000527         4546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_66980, whole genome shotgun sequence.
ACCESSION   DATLVW010000527 DATLVW010000000
VERSION     DATLVW010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66980"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VNN25_15735"
     CDS             <1..209
                     /locus_tag="VNN25_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93033.1"
                     /translation="ETEKMMNDAFKNLRVAWKITAPFEVASTNATRREGNTLIWEYDF
                     AKFQKLAENPKGLDDMGVNVTYKK"
     gene            677..2590
                     /locus_tag="VNN25_15740"
     CDS             677..2590
                     /locus_tag="VNN25_15740"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH93034.1"
                     /translation="MTQYFLSYTRADEKFALRLTDDLRASGVDLWVDQRDIKLSTPWD
                     RAIEAALRSCAAVVVVLSPRAVGSENVLNEVGFALDHGKDVIPVLLEACEIPIRISRL
                     QRVDFTGDYDVALERCRTTLGGIDPELVRRAEGELTAYLGPLASKFVKVDAAKAHDAA
                     ELYRVLGAHILDAEKQAAFLKNAPIRVKVRKRRRPALIPKIAAVALATVLIALFVHSM
                     IQIAQLRNARSEADALAAYDRLRAGERLFVPRLWLVGRWKPQVLIAQALNKRAAKMLA
                     SPLRKQMDDGLLLSAQAAMLSGAKLTDAAQRVYDEQHYESLVTSIEAGEEIAGRSLAV
                     SSNAAEWRIAIGGQIWSCAEPIGQRPCVVFPSSSQLRVVDAAGFDSNTLRLVGYSGEA
                     KTLDLLSGQESSYVVTDATFVAVDQGDVATSFDHGTTGGPSVRVDPAGPSLGPTNSGD
                     FGRVKMLAFGPCRDCIATRGIDGVVKIWHWLSRGAAGNPLILPGEALSLAATRSGHRL
                     ALISKAADHITFYGADAKPEGIGPPIDLSDAESMAISPDGRHVAVVHGGNVKIFDDRA
                     ALRTLIAATKQPTPIAVAFAGNDYLVTRTPNDARVWRLRSTERRDLGPEERWAEWRKK
                     FGLAGAGVVPSKN"
     gene            complement(2492..3517)
                     /locus_tag="VNN25_15745"
     CDS             complement(2492..3517)
                     /locus_tag="VNN25_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93035.1"
                     /translation="MDARQILRILCLASFVMTAAVNAGAKNLAKMVASLLDVVTGYSP
                     ARADDGKYHVIVLYNSALLSDSQLHDGQVYAQRKQINNRDVYAQLVGIDAAERIQCVI
                     LVDLAPQQVIAAEQFARDKKVVIASVLPSSTNIDRGSVPFIFDVQQQVVMVRSASISS
                     YGLNWTSSRPKGVPELAGESRFRNVDSCPPLLPDDRDAYPYVACHDNAFARMNDKSAW
                     HEGVRDLDTAILINFEEKFDARVYPRGWGLKGYHPHIGLADYLSRYGNCEGAQRELKI
                     ALLPKDAKTRAALEQRIKAQCAAHSVAEQRLRWSILARDNAGAGESELLPPFRPTFLW
                     TEITTLR"
     gene            complement(3517..>4546)
                     /locus_tag="VNN25_15750"
     CDS             complement(3517..>4546)
                     /locus_tag="VNN25_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HXH93036.1"
                     /translation="SANHVNNVSFASVVFPLERFLSARRLTIAAYRHELANFAASQAT
                     RGVFFDATDPQTGQRIPGYREYPAVSSLRLRIAGEGIALALRASPRFSLGAGLRLYQS
                     TIDSVTRRYGTQGGTGDPDYSDLQIIQRQHGRGTSIGINAGALIDLTPKLSFGASYRQ
                     GFSFPVAVDYSDFVPYNASPQVPGNQERARPTQTGAFNVPAFYGVGTSFRPTDNWSIA
                     ADVNRITYSDTTRGFVNLFAEEPRYFVPDGTEIRLGSELLLTRDRFAALPFPISIAGG
                     VWRDPDHSIRAADPADAQAVFFRKTSADIHVTGGLGILLGTKAQLHAAVDHSSRQTVV
                     SMSAMARF"
BASE COUNT          892 a         1394 c         1416 g          844 t
ORIGIN      
        1 ccgaaaccga aaagatgatg aacgacgcct tcaagaacct gcgcgtcgcc tggaaaatca
       61 ccgccccgtt cgaggtggca tccaccaacg ccacgcgccg cgaaggcaac actctgatct
      121 gggagtacga cttcgccaag ttccagaaac tcgccgagaa cccgaaaggt ctcgacgaca
      181 tgggcgtgaa tgtgacgtac aagaagtagg tggggtgtgg gagttgatct tgagaggacc
      241 agttggcttc cgtaaaccgc caaggcgtcc atcggcgtca tcgtttgccc gacgcccaac
      301 tactaacctg tccacgaaac cggggcaggc taagactctc cgactcccca catccgaccc
      361 ccactccccg ctaatgacgt gcggctcggc ctcgctgtgc gggcggcggg tcgggcagta
      421 tttacgttcc gacgcgcgac cgtgcggctt ctcttacggg ctgatgagta atcgatgcgg
      481 atcatgacgg taagataatt catcttgccg caagtcgcgg gcgacgtcag tatgggaaaa
      541 aacgtacgtg ccggaatacc agaagcccgg ctccgacgag atcacttccc tctccgcgtc
      601 gttcaccgca tgcgccacac ggcggagccg gcgatgaaga tgcctggcga gagcttcaag
      661 tagcagggac catcgcatga cgcaatactt cctcagctat acgcgcgccg atgagaagtt
      721 cgcgttaagg ctcaccgacg atctgcgcgc atccggggtt gacttgtggg tcgatcagcg
      781 cgacatcaaa ctgagcacgc cctgggaccg cgccatcgag gccgccctgc gcagctgcgc
      841 cgccgtcgtg gttgtccttt cgccgcgcgc agtggggtcg gaaaacgtgc ttaacgaagt
      901 ggggttcgcg ctcgaccacg gcaaagacgt gattcccgta ctgctcgagg catgcgaaat
      961 tccgatccgg atcagccgtc tccagcgtgt cgatttcacc ggcgattacg acgtggcact
     1021 cgagcgctgc agaacgactc tgggcggtat cgatcccgag ctcgttcgac gagccgaagg
     1081 cgagttgacg gcgtaccttg ggccacttgc cagcaagttc gtcaaagtgg acgcggcgaa
     1141 ggcacacgac gccgcggagc tctatcgcgt gcttggcgct cacatcctcg atgccgaaaa
     1201 gcaggcggca tttctgaaaa acgcaccgat tcgcgtcaag gttcgtaagc gacggcggcc
     1261 ggcgttgatc ccgaaaatag ccgccgtcgc cctggcgacc gtgttgatcg ccctcttcgt
     1321 gcactcgatg atccagatcg cgcagcttcg caacgcccgc agcgaggcgg atgcactcgc
     1381 cgcgtatgac cgcctgcgtg ccggcgagcg gctattcgtg ccgcgtctct ggctcgtcgg
     1441 acgctggaag ccgcaagtgc tcatcgcaca ggcgctcaat aaacgcgcgg cgaaaatgct
     1501 tgcctcgccc cttcgaaagc agatggacga tggcctgttg ctttcggcgc aggcggcgat
     1561 gctgagcggc gccaaactca ccgatgccgc gcagcgcgtt tatgacgaac agcactatga
     1621 gtctctcgtg acgtccatcg aagcggggga agagatcgcc ggaagatcgc ttgccgtcag
     1681 ttccaacgcc gcggagtggc gcatcgcgat cggtgggcag atctggagct gcgcggaacc
     1741 aatcgggcag cggccgtgtg tcgtatttcc gagcagctcg cagctgaggg tcgtcgacgc
     1801 ggccgggttc gattcgaaca cactccggtt ggttggatat tcgggcgagg cgaaaacgct
     1861 cgaccttctc tccggccagg agtcatccta cgtggtaacg gatgcgacgt tcgtcgctgt
     1921 ggatcaaggc gatgtggcaa cttcgtttga ccatggcacc acgggaggtc cgtcggtgag
     1981 ggtcgacccg gcaggaccct ctctcgggcc aaccaattcg ggcgacttcg gccgggtgaa
     2041 gatgctggcc ttcgggccgt gccgcgactg catcgcgacc cgcggaattg acggcgtcgt
     2101 gaagatctgg cactggttgt cgcgcggtgc cgctggcaat ccgctcatcc tgccgggcga
     2161 ggcgctctcg ctggcggcga ctcgctcggg acatcgcctg gcgttgatca gcaaagcagc
     2221 cgaccacatt accttctacg gtgccgatgc gaaacccgag gggatagggc cgccgatcga
     2281 cctgtccgac gccgaatcga tggcaatctc tcccgacggc cgccacgtcg ccgtcgttca
     2341 cggcggcaac gtgaagatct tcgacgaccg cgcggccctc cggacgctga ttgctgcgac
     2401 gaaacaaccc acaccgatcg ccgtcgcctt cgccggaaac gactatctgg tgacgcgcac
     2461 acccaacgac gctcgtgtct ggaggcttcg ctcaacggag cgtcgtgatc tcggtccaga
     2521 ggaacgttgg gcggaatggc ggaagaagtt cggactcgcc ggcgccggcg ttgtcccgag
     2581 caagaattga ccaacggagc cgttgttccg ccacggagtg cgccgcacac tgggccttga
     2641 ttctctgctc gagcgcggcc ctcgtcttcg catctttcgg cagcaaggcg attttcagct
     2701 cccgctgcgc cccttcgcag ttgccgtatc ggctcagata gtcagccagg ccgatgtgcg
     2761 ggtgatatcc cttcaggccc catccccggg gatacaccct ggcgtcgaac ttttcctcga
     2821 agtttatgag gatcgcggta tcgaggtcgc gcacgccttc gtgccacgcg ctcttatcgt
     2881 tcattcgggc gaaggcattg tcatgacacg cgacgtaggg ataagcatcc cgatcgtcgg
     2941 gaagcagcgg cggacagcta tcgacgtttc ggaatcgcga ctcgccggcc agttcgggca
     3001 cgcctttcgg ccgcgagctc gtccaattga ggccgtaaga gctgatcgaa gcgcttcgca
     3061 ccatcaccac ctgctgctgc acgtcgaaaa tgaacggcac gctaccgcgg tcaatattgg
     3121 tcgaggaagg gagcacagat gcgatcacca ctttcttgtc gcgagcgaat tgctcggcgg
     3181 cgatgacctg ttgcggcgcg agatcgacca ggattacgca ttggatgcgc tccgccgcat
     3241 cgattcccac taattgcgcg tacacgtctc ggttgttgat ttgcttccgc tgggcataaa
     3301 cctgtccgtc atgtaactgg gagtcactca ggagtgcgct gttgtagagc acgatgacgt
     3361 ggtacttgcc gtcgtcagca cgcgccggcg aatagcccgt aacaacatcc aggaggctgg
     3421 cgaccatctt cgccaggttc ttcgcgcctg cattgaccgc ggctgtcatg acaaaacttg
     3481 cgagacagag aatgcgaaga atttgacgcg cgtccatcag aacctcgcca tcgccgacat
     3541 cgaaacaact gtttgccgcg acgagtgatc gacggctgcg tgaagctgcg ccttcgtccc
     3601 gaggaggatt ccgagcccgc ccgtgacgtg aatgtcggcg gaagtcttcc gaaagaagac
     3661 cgcctgcgca tccgcgggat cggcggcgcg aatactgtga tccggatcgc gccatacgcc
     3721 gccggcaatc gagatcggga acggcagcgc cgcgaaacgg tcccgcgtca gcaacagttc
     3781 ggagccgaga cggatctccg ttccatccgg gacgaagtag cggggctcct cggcgaacag
     3841 attcacgaaa ccgcgggtgg tatcggaata ggtgatcctg ttgacatcgg ccgcaatcga
     3901 ccagttgtcc gttggacgga acgacgtccc gacgccgtag aacgccggca cgttgaacgc
     3961 gccagtctgt gtgggccggg ctcgctcctg gttcccggga acttgtggag aagcgttgta
     4021 tgggacgaaa tcggagtagt cgacggccac cggaaacgaa aatccctgcc gatagctggc
     4081 tccgaaactc agtttcggcg tgaggtcgat cagcgcgccg gcattgattc cgatcgacgt
     4141 cccgcgcccg tgttggcgtt ggataatttg caagtccgag tagtctggat cgcccgtgcc
     4201 tccctgcgtc ccgtaccgcc gcgtgaccga gtcgatcgtg gactgataca agcgaagccc
     4261 ggctccgagc gagaatcgtg gtgaggcacg caacgcgagc gcgatgcctt cgcctgcaat
     4321 gcgaaggcga agagagctga ccgccgggta ctcgcggtag ccggggattc gctgcccggt
     4381 ttgcggatcg gttgcatcga aaaagactcc cctcgttgcc tgcgatgccg cgaaattcgc
     4441 caactcgtgg cggtaggcgg cgatcgtcag ccgtcgcgcg ctcaaaaacc gttcgagagg
     4501 aaagacaacc gaggcgaagg agacgttgtt cacatgattt gcggac
//
LOCUS       DATLVW010000528         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_601535, whole genome shotgun sequence.
ACCESSION   DATLVW010000528 DATLVW010000000
VERSION     DATLVW010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601535"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1299)
                     /locus_tag="VNN25_15755"
     CDS             complement(187..1299)
                     /locus_tag="VNN25_15755"
                     /inference="COORDINATES: protein motif:HMM:NF022664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HXH93037.1"
                     /translation="MRRWFVTCVVLLSCAAPALAQSITCESPEGGYRECRGGSAGKAE
                     LTLELSDNRCIEGTSYGTRMEGVVWVDRGCRGRFSFRGGSLTGNRRIICESQKGGLEW
                     CPAELNNAPDPKVGVSLVRQLGEKACVEGDGWGYDIERDQIWVDKGCRGEFMVGHANR
                     SKAVEALDAKVVCETPNRGKVECTADTGAGVRIIRTLNDTTCGFGRQWGYDEKSIWVS
                     GGCRAEFAIRGVAKPTIQAVTCESHNNVRVACPAETKYGVALVRNTGENECILGKSWG
                     FDDKGVWVAENCRAQFALGGYRLPATAKLPPTAMKLVCESADGQKKICEVDASHGVGL
                     LRQISETDCVLNRSWGYGRDGIWVSEGCKAEFVVAK"
     gene            1507..1842
                     /locus_tag="VNN25_15760"
     CDS             1507..1842
                     /locus_tag="VNN25_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HXH93038.1"
                     /translation="MSEHYYESKHLGAFGDIAEGAPDLAKKFFEYYNAVFAAGALTER
                     EKALIALAVAHAVQCPYCIDAYTTTSLEKGADLEQMTEAVHVACAIRGGASLVHGMQM
                     KGHAKKAGM"
     gene            1867..2967
                     /gene="arsS"
                     /locus_tag="VNN25_15765"
     CDS             1867..2967
                     /gene="arsS"
                     /locus_tag="VNN25_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710881.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HXH93039.1"
                     /translation="MDSTEKATATAIDGPRAAQSLRARHSTLAAPAEQLRQLANVVIE
                     GQPLDFEQSIAAAGFAGLHPAPLEIFQINLGKLCNMTCRHCHVDAGPDRTDAMMSLDT
                     IEACLRALDQTTAHTVDLTGGAPELHPHFRYVVEESVRRGKHVIDRCNLSVLLLPRNK
                     GLVEWLGERGVEIVASLPHYRKRNTDAQRGDGAFDKSIEALHLLNEAGYGTGDPRRRL
                     TLMSNPAGAFLSANQASIESEWKEGLRRNFAITFDRLFTLNNMPISRFLEWLVDSENL
                     DPYMERLAKSFNPAAINGLMCRNTISISWDGRLYDCDFNQMLDLDALPDAAAMHVTEF
                     DLAKWQSRRIVTRRHCFGCTAGSGSSCGGATT"
     gene            complement(3053..>4643)
                     /locus_tag="VNN25_15770"
     CDS             complement(3053..>4643)
                     /locus_tag="VNN25_15770"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXH93040.1"
                     /translation="NPMEAHGLIAAWDGDHLTLHDSSQNIFAVQRTFATLFGIPKENV
                     HVLSPFVGGAFGSKGGVWPHVVIAAMAARVVGRPVKLTVTRKQLFFANGHRPQTEQRV
                     ALGAAADGKLVSVIHEGFGQGNEVCSFVEAFTAPTRVIYASPNMRGFHHVVDLNLPSG
                     TYMRGPGDSTGMFALDSAMDEMAYALKIDPLQFRLINHADQDPLSGKPWSSKSLKECY
                     SRAAERFGWSRRTAEPRSMRDGRNLIGWGMASANRPAHRAPCSARALMRADGTVTVSC
                     GSHEMGMGTATVMAQLAAETLGIPVERVRFEYGDTKLPTAPISAGSLTAASVGCAVFG
                     AATELKKKLGELAGADPASLDPNNYTAILRQHYLDHVDAQFDAKPPMEDPYSSHAFGA
                     HFVEVAIDEDIPVVRVRRIVSAFAAGKILNEKTARSQCYGGIVWGLGQALLEQTHVDT
                     KLGSFTNVNFAEYLVPVNPDIQSIEVILVEETDPHINPIGVKGIGEVSIVGVAPAIAN
                     AVFHATGKRVRELPITVEKLL"
BASE COUNT          819 a         1505 c         1478 g          841 t
ORIGIN      
        1 cggagcagtc gccatatcta gtctccgaca cccgacaacc gacacccgac accctcactt
       61 cgggacctag aacgcggggc tcgggagtcg gagcagtggc tgatcatcag cagaaatctc
      121 tcccacaccc cactccccac acccgactcc ctcgctccga cacccgaccc ccgacaaccg
      181 acaccctcac ttcgccacca cgaactccgc cttgcagcct tctgacaccc agattccgtc
      241 gcggccgtag ccccagcttc gattgaggac gcagtcggtc tcgctgatct ggcgcagcag
      301 gcccacgccg tgcgaagcat cgacctcgca gatctttttc tggccgtcgg ctgactcgca
      361 gacgagcttc atcgctgtgg gaggaagttt cgcagtcgcc ggcaagcggt aaccgccgag
      421 ggcgaattgg gcgcggcagt tttcagcgac ccagacgcct ttgtcgtcga agccccagct
      481 cttgccgagg atgcattcgt tctcaccggt gttgcgcacg agcgcgactc cgtatttcgt
      541 ctccgcgggg caggccacgc ggacgttgtt gtgcgactcg caggtgacgg cttggatggt
      601 tggtttggcg acgccgcgga tcgcgaactc cgcgcggcaa ccgccgctga cccagatgct
      661 cttctcgtcg tagccccact gccgcccgaa gccgcaggtg gtgtcattca gcgtccggat
      721 gatgcgcacg ccggccccgg tatccgcggt gcattccacc ttccctcggt taggcgtctc
      781 gcagacgact ttggcgtcga gcgcctccac ggctttcgag cgattggcgt gaccgaccat
      841 gaattcgccg cggcatccct tatcaaccca gatctgatcg cgctcgatgt cgtaacccca
      901 gccatcgccc tcgacgcacg ctttttcgcc gagctgccgg acgagcgata cgccaacctt
      961 cggatcgggg gcgttgttca attcggcggg gcaccactcc agtccgcctt tctgcgactc
     1021 gcagatgatg cgccggttgc cggtgaggct gccgccgcga aacgagaagc gtccgcggca
     1081 accgcgatca acccacacca cgccttccat gcgagtgccg tagctggtgc cctcgatgca
     1141 gcggttgtcg ctgagctcca gcgtcagttc ggctttgccg gcagatccgc cgcggcattc
     1201 gcggtagcct ccctccggcg attcgcaggt gatcgattgc gccaacgccg gcgccgcaca
     1261 cgacaagaga accacacacg taacgaacca tctgcgcatc gctgcctccg gacttgctga
     1321 tcatctgcgc ttccgggtcc ggggtctagt gctgattgct cgtcggcggc ggcgtcgcga
     1381 gagccggcag cacaacgatc tcgcctctcg cggatggaac gctggcgtcc tcgccggctg
     1441 gctcggaggc gtctcgcctc cgttgcgcac accgtcatag actccgcccc gaaggagaaa
     1501 cccaccatgt ccgagcacta ttacgaatca aaacatcttg gcgcctttgg cgacattgcc
     1561 gagggtgctc ccgatctcgc gaagaaattc ttcgagtact acaacgccgt tttcgcggcc
     1621 ggtgcgctca cggagcgtga gaaggcgttg atcgcgcttg ccgtcgcgca tgcggtccag
     1681 tgtccgtact gcatcgacgc ctacacgacc acgagtctcg aaaaaggcgc cgacctcgag
     1741 cagatgaccg aggcggttca tgtggcgtgt gcaatccgag gaggagcatc gcttgttcat
     1801 gggatgcaga tgaagggaca cgcgaagaaa gcggggatgt gaagaagggt gaagggatcc
     1861 acgatcttgg actcgactga gaaagcgacg gcaaccgcga tcgatggtcc gcgcgcggct
     1921 caatcgctgc gcgcgcggca ttcgacgctt gcggcacccg cggagcagtt gcgccaactt
     1981 gccaacgtcg tgatcgaagg tcagccgctc gatttcgagc agtcgattgc cgccgccggt
     2041 ttcgccggcc tccatcccgc gcccctcgaa atcttccaga tcaacctcgg caagctttgc
     2101 aacatgacgt gccgtcactg ccatgtcgac gcggggcccg accgaaccga cgcgatgatg
     2161 agtctcgaca cgatcgaggc ctgtctgcgc gcgctcgatc agacgacggc gcatacggtc
     2221 gatctcaccg gcggcgcgcc ggagctgcac ccgcactttc gctacgtcgt cgaggaatcg
     2281 gtacgccgag ggaagcacgt cattgatcga tgcaacctgt ctgtgctcct gctgccgcgc
     2341 aacaagggac tcgtggagtg gcttggcgag cgcggcgtgg agatcgtggc gtcgctgccg
     2401 cactatcgaa agcgcaacac cgacgcacag cgcggcgatg gcgccttcga caaatcgatc
     2461 gaggcgctcc atcttctgaa cgaagccggc tatggcacag gcgatccgcg gcggcgtctg
     2521 acgctgatga gcaatccggc cggcgcgttc ctctccgcga atcaggccag catcgagagc
     2581 gaatggaagg aaggcctgcg gcgcaatttc gcgatcacgt tcgatcgcct gttcacgctc
     2641 aacaacatgc cgatctcgcg gtttctggag tggctggtcg acagcgagaa tctcgatccg
     2701 tacatggagc ggctggcaaa gtcgttcaat ccagcggcga tcaacggctt gatgtgccgc
     2761 aacacgatct cgatctcgtg ggacggccgg ctctacgact gcgatttcaa ccagatgctc
     2821 gatctcgatg cgctgccgga tgcggcggcg atgcacgtga ccgaattcga tctcgccaaa
     2881 tggcagagcc gccgcatcgt cacccgccgc cactgcttcg gctgcacggc cggatcggga
     2941 agttcgtgcg gtggggcgac gacataaaac cgtttcacgc ggaggcgcgg agatgcggag
     3001 aaaacccctc cgcgttcccc gcgtctccgc gtgaaacggt tttttcagaa cgtcacagca
     3061 gcttctcaac cgtgatcggc agctcgcgca ctcgctttcc cgttgcgtgg aaaacagcgt
     3121 tcgcgatcgc gggcgccaca ccgacgatgc tcacttcgcc gattcccttg acgccgatcg
     3181 ggttgatgtg cgggtcggtc tcttcgacga ggatcacctc gatcgactgg atgtcggggt
     3241 tcaccggcac gaggtactcg gcgaagttga cgttggtgaa ggagccgagc ttcgtgtcga
     3301 cgtgcgtctg ctcgaggagg gcttggccga ggccccagac gatgccgccg taacattggc
     3361 tgcgcgcggt tttctcgttc aggatcttgc cggcggcgaa ggcgctgacg atgcggcgga
     3421 cgcggacgac cgggatgtct tcgtcgatgg ccacctcgac gaaatgcgcg ccgaacgcgt
     3481 gcgacgagta cggatcttcc atcggcggct tggcgtcaaa ctgagcgtcg acgtgatcga
     3541 gatagtgctg ccgcaggatg gccgtgtagt tgttgggatc cagggaggca ggatcggcgc
     3601 ctgccagttc gcccagcttc ttcttcaact cagtggccgc accgaagacg gcgcagccga
     3661 cgctggctgc agtcagcgat ccggcggaga tcggcgcggt gggcagcttc gtgtcgccgt
     3721 attcgaagcg gacgcgttcg acagggattc cgagcgtctc ggcggcgagc tgcgccatca
     3781 cggtcgcggt tcccatcccc atctcgtgcg agccgcagct caccgtgacg gtgccgtcgg
     3841 cgcgcatgag ggcacgcgcc gagcagggcg cgcggtgcgc cggacggttg gcgctggcca
     3901 ttccccagcc gatgagattg cggccgtcgc gcatcgagcg cggctcggcg gtccgccgcg
     3961 accagccgaa acgctcggca gcgcgcgagt agcattcctt gagcgacttg ctcgaccacg
     4021 gcttgcccga cagcggatcc tgatcggcgt gattgatcag gcggaactga agcggatcga
     4081 tcttcagcgc gtaggccatt tcgtccatgg ccgagtcgag cgcgaacatg ccggtgctgt
     4141 cgccggggcc gcgcatgtat gtgccggacg ggagattgag atcgacgacg tgatggaagc
     4201 cgcgcatgtt cggcgacgcg tagatgacgc gcgtcggcgc ggtgaaggct tcgacaaatg
     4261 agcagacttc gttgccctgc ccgaaaccct cgtggatgac ggagacgagc ttgccgtcgg
     4321 ccgcagcgcc gagggcgacg cgctgctcgg tctgcgggcg atggccattg gcgaagaaga
     4381 gctgtttgcg cgtgacggtc agcttcaccg ggcgcccgac gacgcgtgcc gccatcgccg
     4441 cgatgacgac gtgcggccag acgcctcctt tgctgccgaa cgcgccgccg acgaagggcg
     4501 agagcacgtg aacgttttct ttcgggatgc cgaacagcgt tgcgaaggtg cgctgcaccg
     4561 cgaagatgtt ctgcgacgag tcatgcagcg tgaggtgatc gccatcccac gccgcgatga
     4621 ggccgtgcgc ctccatcggg ttg
//
LOCUS       DATLVW010000529        12816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_149373, whole genome shotgun sequence.
ACCESSION   DATLVW010000529 DATLVW010000000
VERSION     DATLVW010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149373"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNN25_15775"
     CDS             complement(<1..207)
                     /locus_tag="VNN25_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93041.1"
                     /translation="MRFRPVLFVGLGRYGCEIARRICTTVREMNAEASPIVALVEIHD
                     DGSVVLEEGGEGDGEALHVKFAEAT"
     gene            complement(217..2901)
                     /locus_tag="VNN25_15780"
     CDS             complement(217..2901)
                     /locus_tag="VNN25_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HXH93042.1"
                     /translation="MTHSLKKTAVIGLGGTGAQAVLYMKQKLIATYGEIPPMIKFLVI
                     DTTELADVADGDVTLDPGEFLKLEVREPGALMSRNAEIREWLPENIPRFALSAGAKQV
                     RALGRVAVFANSGPVENKINGLIGALRDFKVGRDEKFEVMTDNVLVNIVCSLSGGTGS
                     GAFLDVAVLARKQLQSVDKMIGYFLMPDIFVGKPATDNVEANAYGALKEISYLFEHPH
                     FRYNFGGRPRAVEGEALFNGIYLVNKSNSKAIEYNNMADLQEFLGIGMFLQSTATGKG
                     ASDIIDNLEAQIVSHKWFDKPTVFSSFGVSELVYPGDWYAELYAKKIAFGCLNGVLVG
                     GDVSAVDEYTEGFVRRIQINEHEADDVINALGSPTDVPQFTVPREVNRAAVQALLNKR
                     DTHLAEVHRGVHDSTADKLASLRRKKSGALDEDVRKILSRPQGLQFAISFLATLQGKL
                     LGFKKELTDERQAKELDKSGLATRYEPTKSEIEKLAAAFFPSKNDLQQALKRYKGIID
                     REAQLIMEIERRDRGIDFLAYMAENTTSWLDRLNALSGMCSSVTQELSQDIQQMRQTK
                     RAPRPFVQELKPKNLTEDAPGVDPNDFLRWLAEDKKMTIVDLAGMRVSELKSTLLEYG
                     YADENVTEVRDRRIEDILRGLSKNERNRLINELDSMAAPLWQYDRGTISGELHTENIY
                     LFGVEDRDDTTLAPDLVQQVVQSYYPPSVVSTGDPKRIVCFKVESAVPAFVVYNVGRY
                     RESYRRPNRPFSNHIDREWEKLPDLFPGEGQEDAMKYWSLALADPFSLIVKRGEVYYL
                     KSERFGERTKDYLVKLAMGREKAMRVFVEDPERVKDVRESIERINERVGNDTIAEQLR
                     TYTAKLELKAKKQSEEIRRQIEGELSQVDAYIKSISSL"
     gene            complement(2924..4708)
                     /locus_tag="VNN25_15785"
     CDS             complement(2924..4708)
                     /locus_tag="VNN25_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXH93043.1"
                     /translation="MIIALATALLALRVAAQPMASPPAHLKIDYVFLIDVSGSMKGMA
                     GHPNIFPAVKNAIAGFIAKLDPGTTVYFVPFAERVREIRPFALHGPSEVAEATAYLNT
                     LDADGLNTAIYNSIRDLMDYMKGRRPTPEQAAANPLIILVYTDGDDNVSQGLTLESIL
                     KEYNLRRGSHDWIFYTELGLPQNPQKAATFSRFDNALYVAERPGEIRPITVIEARLPF
                     MNFGNVLETQSPERDQQFVVRGTQRLPANFSLRVEPNFDKLRSAGVLADIEPERLPVT
                     EKKVKLKLTLSNVENLAHGVYKGKLAVTPSDPLVIVVPDDISAEFSYEPPKKIVISAA
                     PGEHLPIDLGDVAPDRTATAHLRIAADEEARSVKIPVTFHIADFTGNPARLALGSAVY
                     IDGQRGKRDGAIAAPSEVTFVIQPPPNVPVGDYSGTIDISAPGAQLSYEGGSPTGATL
                     QIPWRFHIPPPPTPWWVWAIVVLVAISAILLLVYVATRPPRFSDLQLQMLKPRHGTID
                     LSGLSKKSFGPSSSDLTDSKADFSIVARKRDGGGVDALIETESNDVKLQRRGRNVDIF
                     QQEELHAGDVIVVDQYELRVESTALNEA"
     gene            5396..6328
                     /locus_tag="VNN25_15790"
     CDS             5396..6328
                     /locus_tag="VNN25_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93044.1"
                     /translation="MGEGTELVGYATFLFFAYIYLPYAFLKFGAEQSIDLGKRRDFTQ
                     FEEIISGFVPAFVLNLLASLLYLGVTRCRYRLDFSVMASMFGSNRGAVSDYIYAGDWA
                     GCLIYLFVLWGVAYANGYWFGAAVHEIAETNPLALHGIERVWEEWETHRRKVPATAKR
                     NPWTRFRSGFLWLGWWLWYRFFQESIVPLYTWIAQHPIVLVETARYDRFLGRFIRYEK
                     TTDGQFDAIRLEVASRLPSRRRASSNRKKTTPEDSKCVPIGMFYLKWSEIASIVVIGP
                     ADSENCETAIALFTWWTRNTTSMDSVTPESGGDA"
     gene            6538..7143
                     /locus_tag="VNN25_15795"
     CDS             6538..7143
                     /locus_tag="VNN25_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93045.1"
                     /translation="MTDLELEDFESLQIQANKLGIQGFAIAVGKRGRYRRFDLPPAKT
                     GKARGSVGEGNRVRLKGYIVGVPHARRTDSANCRLVGRANNNYVIHIAFLADDTEFDS
                     VVAEMIAQRRDLNWNLEKLRKIATDKRPVKITGQLFYDNKHLVNSDPDEELPGHPKRL
                     SLWEIHPVTELEVCNEYPEKLDQCFEKGAIWTPLAKWVSKQ"
     gene            7107..8474
                     /locus_tag="VNN25_15800"
     CDS             7107..8474
                     /locus_tag="VNN25_15800"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXH93046.1"
                     /translation="MDTAGEMGIETVMTPRARCALLFAGMLLCSKEALASSLVTPVDC
                     APSGVKVHAGPSLNAPRLAKLPFGQRLRSLGRPQDGWYEVVLRNGRHGFSCGELLHEV
                     PANDIEFTVRFMDVPGGEATIINVGDTDILIDGGSDPDSLLRYLHEGHLIRGPLELAI
                     VTDQDSDHWNGFRCLRGLSGKLPFRILELWVPDQDNWCSPADAEELGVISSSLPGTCV
                     LKHSVVVGNAKPPSNCKVREISYHPRNPTAVDVKLPFAPEVDLTILRPFESAENDCAS
                     RSNDSSLVFIATIGASRFLFTSDQRGKKEGDANRQEVQYGEKQLIEMANQEPGLLTAN
                     VLKVANHGADTSTMPGFISRVFPRDSTGPKFAVICSANHTKQDVVVKRCQRRAFVLST
                     DERLGAQKGDVVCIKRKGEKSLDCAHEPRLYRLPPIDVPHPSNKNPTMECAPCQAKTS
                     GHQNR"
     gene            complement(9808..11598)
                     /locus_tag="VNN25_15805"
     CDS             complement(9808..11598)
                     /locus_tag="VNN25_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXH93047.1"
                     /translation="MPPVLLTCERLTKSYTSRPLFNDLSFSLFEGDHVGLVGPNGSGK
                     STLMKIVAGVEEADSGNRAVRKGVRIGYVPQDPVFAHGKTIEDVLLEGLENDHILDDY
                     GKQTRVAIALGKSGFMDRTQDTATLSGGWRKRLAIARELAREPDVMLLDEPTNHLDVD
                     AILWLEGLLKSEPKAFVVVSHDRYFLENTARRMLELNRQYADGLLQVNGHYSDFLERR
                     DELLRNEAAYQETLANLVRREVEWLRRGPKARTTKSKSRIQNAEGLIGELADSRSRSN
                     TSTAKIDFTASERRTKRLWLVKGLRKSMGDKLLVADLDILLTPGSRLGILGPNGSGKT
                     TLLRTITGELQPDAGTIEKAEKLRIVYFEQNRESLDPTLTLKRALAPEGDQVVYRDRT
                     LHVASWAKRFLFQPDQLETSVSKLSGGEKARIVLARLMLQPADLLVLDEPTNDLDIST
                     LEVLEDSLLDFPGALVLVTHDRYLIDRVSTQILALDGKGGAEYFADYTQWQENRPRPA
                     APTRDARPSKPKAKKLTYAEQREWDAMEANVHAAEERLDAARVAVEDPTVATDATILQ
                     QRVTALDTAQDTVDRLYARWAELEGKRGGE"
     gene            11722..>12816
                     /locus_tag="VNN25_15810"
     CDS             11722..>12816
                     /locus_tag="VNN25_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93048.1"
                     /translation="MHEFIAKHRNKIAGILSGFDRLVFRGTLRSLSFVDGMRHYLGAN
                     DVLLKDFGPHVERVSQQLKDASRGLVKQLNRPLNHLPSSKLSKEKIAREVVARDGIRE
                     GLICVLTCVEPCWSFEVHRNRETKKLELESRYRKCEFIYQYWMHPKFGFMNARIQTWF
                     PFQIQICMNGRMWLGRQMDQAGIQYAAADNCFPWIEDWTKAQAVMDQQLSTDWPKALE
                     PIAQQLNPVHGEMFRKYPVSYYWSTYQSEWATDIVFQKAKELRRLFPRLVHHGITTFS
                     CTDVMRYLGKPTLLSGEVPRRFSGDVVSDIKHREEGVRLKHTINGNSVKLYDKAFTKP
                     GSVLRAETTIQNGSDFQVYRPKEGDPDGKMS"
BASE COUNT         2485 a         3695 c         3949 g         2687 t
ORIGIN      
        1 tgtcgcctcc gcgaatttga cgtgcagcgc ctctccgtcg ccctctccgc cttcctcgag
       61 aacgacgctg ccgtcgtcgt ggatctccac gagcgcaaca atcggcgacg cctcggcgtt
      121 catctcccgg acagtagtgc agatgcggcg cgcgatctcg catccgtaac ggcccaaacc
      181 gacgaagagt acaggtcgga atcgcatgtg agctccctat aggctgctga tcgatttgat
      241 atatgcgtcg acctgggaaa gctcgccctc gatctgcctg cgaatttctt ccgattgttt
      301 cttcgccttg agctcgagtt ttgcggtgta ggtgcggagc tgttcggcta tggtgtcgtt
      361 gcccactcgc tcgttgattc gctcgatcga ctcgcggacg tccttcaccc gctccgggtc
      421 ctcgacgaat acccgcatgg ccttctcacg tcccatggct agtttcacca gataatcctt
      481 ggtacgctcg ccgaaccttt ccgatttgag gtagtacacc tcgccgcgct tcacaatgag
      541 gctgaacgga tcggccaggg cgagcgacca gtacttcatc gcatcctctt gtccctcgcc
      601 ggggaagagg tccggcagct tctcccactc gcgatcgata tgattggaaa acgggcggtt
      661 cggacgtcgg taagactcgc ggtagcgccc gacgttgtaa acgacgaacg ccgggaccgc
      721 cgactcgact ttgaagcaaa cgatgcgctt cgggtcgccc gtggaaacaa ctgacggcgg
      781 atagtacgac tggaccacct gctgaacgag atcgggcgcg agtgtcgtgt cgtcgcggtc
      841 ctcgacgccg aacaggtaga tgttctccgt atgcagttca cccgagattg tgccgcggtc
      901 atactgccag agcggggctg ccatcgaatc gagttcattg attagccggt tgcgctcatt
      961 cttcgagagg ccgcgcaaga tgtcctcgat acggcggtca cggacttcgg tcacattctc
     1021 gtcggcgtag ccatactcga gcagcgtcga tttcagttcg gagacgcgca tgccggctag
     1081 atcgacaatc gtcattttct tgtcttcggc gagccagcgc aggaaatcgt tcggatcgac
     1141 cccgggcgca tcctcggtga gatttttcgg tttgagctcc tggacgaagg gtcgcggcgc
     1201 gcgcttggtc tggcgcatct gctgaatgtc ttggctcagc tcctgtgtaa cgctcgaaca
     1261 cattccactc agcgcattga gccgatccag ccaactcgtg gtgttctctg ccatatatgc
     1321 gaggaagtcg atgccgcgat cacgccgctc gatttccatg atgagctgcg cttcgcggtc
     1381 gatgatgccc ttgtaacgct tcagcgcttg ctgcaggtcg ttcttgctcg ggaagaacgc
     1441 ggccgcgagc ttctcgatct cgctcttcgt cggctcgtag cgcgtagcaa gaccgctttt
     1501 gtccagctct ttcgcctgac gctcgtcggt gagctccttc ttgaatccga ggagctttcc
     1561 ttgcagcgtc gccaggaagc tgatggcgaa ttgcaacccc tgcggccgtg acagaatctt
     1621 gcggacatct tcgtcgagcg cgccgctctt tttgcgtcgc agcgacgcca gcttgtcagc
     1681 ggtcgagtcg tgaacgccgc gatgcacctc cgcgagatgt gtgtctcgct tgttcagcag
     1741 cgcctgtact gccgcgcgat tgacctctcg cggcacggtg aattgcggca cgtccgttgg
     1801 cgatcctaac gcgttgatca cgtcgtccgc ttcgtgctcg ttaatctgaa tgcgtcgtac
     1861 aaagccctcg gtgtattcgt cgaccgccga cacatcaccg cccaccaata cgccgttgag
     1921 gcagccaaaa gcgatctttt tcgcataaag ctcggcatac cagtcaccgg gataaacaag
     1981 ctcgctgaca ccgaagctgc tgaagaccgt cggcttgtcg aaccatttgt ggctgacgat
     2041 ctgcgcctcg aggttgtcga tgatatcaga tgcacctttg cccgtcgctg tgctctgcag
     2101 gaacatgcct atccccagga actcctgtag atcggccatg ttgttgtact cgatggcctt
     2161 cgagttgctc ttgtttacga ggtagatgcc gttgaagagc gcctcgccct cgacggcacg
     2221 agggcggccc ccgaagttgt agcggaaatg cggatgctcg aaaagatagc taatctcttt
     2281 cagcgcgcca tatgcgttgg cctcgacgtt gtcggtggcc ggcttgccga cgaaaatatc
     2341 aggcatcaga aaatacccga tcatcttgtc gacggactgg agctgcttgc gggcgagcac
     2401 ggccacgtcg aggaacgcgc cggagccggt tccgccggag agcgagcaga cgatgttgac
     2461 taggacattg tccgtcatta cctcgaactt ttcgtcgcgg ccgaccttaa aatcgcgcag
     2521 cgcgccgatc agaccgttga tcttgttctc gactgggccg gaattcgcga acactgccac
     2581 gcggccgagg gcgcgcactt gcttcgcacc agcggagagc gcgaagcgcg gaatgttttc
     2641 cggcaaccat tcccgaatct ccgcattgcg ggacatcaga gcgcccggct cgcgaacctc
     2701 gagcttaaga aactcgccgg gatcaagcgt gacgtcgccg tcggcgacgt cggccagctc
     2761 ggtggtgtcg atgaccagaa acttgatcat cggcgggatc tcgccgtacg tcgcgatgag
     2821 cttctgtttc atgtacagca cggcctgggc gccggtgccg cctaatccga ttacggcagt
     2881 cttcttcagg gaatgtgtca tgagatctcc tagcgatcgg cgctcacgct tcgttcaacg
     2941 cggtgctttc tactcgcaat tcgtactgat cgacgacgat gacgtcgccg gcatgaagtt
     3001 cttcctgctg gaaaatatcg acgttcctgc cacgacgctg gagcttgacg tcgttcgact
     3061 ccgtctcgat cagggcgtcc acgccgccgc cgtctcgttt acgtgcaacg atgctgaagt
     3121 ctgccttgct gtccgtcaaa tcactcgagc tcggtccgaa tgacttcttc gagaggccgc
     3181 tcaggtcaat ggtgccgtgg cgaggcttaa gcatttgcag ttgcagatcc gaaaaccgcg
     3241 gcggccgcgt tgcgacgtag acgagcaaaa ggatcgcgga aatcgcaaca aggacgacga
     3301 tagcccaaac ccaccagggc gtcggaggcg gagggatgtg aaaacgccaa ggaatttgca
     3361 gcgtggcgcc ggttgggctc cctccctcgt acgagagctg ggctcccggt gcggagatgt
     3421 cgatcgttcc agagtagtca ccgaccggca cgttcggcgg tggctggatg acgaaggtga
     3481 cctcggacgg tgctgcgatg gcgccgtccc gttttccgcg ctgaccgtcg atatataccg
     3541 cgcttccgag cgccagccgc gccgggttcc cggtgaagtc ggcgatgtgg aaggtcaccg
     3601 gtattttgac actccgcgct tcctcgtccg ccgcaatgcg caggtgcgcg gtcgcagtcc
     3661 ggtcgggagc gacgtcgccg aggtcgatcg gcaggtgctc tcccggcgcc gcactgatca
     3721 cgatcttctt cggcggttcg tagctgaact cggccgagat gtcatccgga acgacgatca
     3781 cgagtggatc gctgggcgtc accgccaact tgcccttgta gacgccatgc gcgagattct
     3841 cgacgttcga caacgtcaac tttagcttga ccttcttctc ggtgacgggg aggcgctcag
     3901 gttcgatgtc ggcgagcacg cctgcggacc gaagcttgtc gaagttcggc tcgactcgca
     3961 gagagaagtt cgcgggcagc cgctgcgtgc cgcgcacgac gaactgttga tcgcgctccg
     4021 gcgactgcgt ctccagaacg ttgccgaagt tcatgaatgg caatcgcgct tcgattacag
     4081 taatcggacg aatctcaccg ggtcgctcgg cgacatagag cgcgttgtcg aaacggctga
     4141 aggttgctgc cttctgtgga ttctgcggaa ggccgagctc agtgtagaag atccagtcat
     4201 gcgatcctcg ccgaaggttg tactctttga gaatgctctc cagcgttaga ccttgagaga
     4261 cattgtcgtc cccatcagtg tagacgagaa tgatgagcgg attggccgct gcttgttccg
     4321 gggtcggtct gcggcccttc atgtagtcca tcagatcgcg gattgagttg tagattgcgg
     4381 tattcaaacc atctgcatcg agggtgttga ggtaggcggt tgcctccgcc acttcgctgg
     4441 gtccgtgaag tgcaaaaggg cggatctcgc ggacccgctc ggcgaatggg acgaagtaca
     4501 ccgtagtccc cgggtcgagc ttggcgatga agccagcaat ggcatttttt accgccggaa
     4561 aaatatttgg atgcccggcc atgcctttca tcgagcccga cacatcgatc agaaacacgt
     4621 agtcgatctt cagatgtgcg ggcggcgacg ccatcggctg cgcggcgaca cgcagcgcga
     4681 gaagcgcagt tgccagggcg ataatcatcc gacgcaactt catcgtattg ggtgcctcac
     4741 ttcgacagcg cggtgttctt tgaccgatcg cgagccagac cgtctcttct cgtctcttct
     4801 aaggagtgac gcagaaagag atttccctac cccggcatag ctgaaaccgg aaaagaatac
     4861 ctctgagcta cgctttcgcc aaccgcacga ccaagcgggg ccagatcagg aaggtcgcgc
     4921 tccaccgccc agtcgaggcg cgcgttcaac tcggtcactg cgaagctgtc agacagatcc
     4981 tgagccttcc gacagttgac gatcccgacg aaggctttgt ccggcatctg gaacgcactc
     5041 ccgcggcacg gctcccccga gtagggtgaa gtagtcgaac atccgaatgt cgcggaatcg
     5101 accgtcggcg ccattgtccg tcgtgacaaa cgcgggcaac gttctttgaa tcacgcctcg
     5161 gtcgaagcgg gagagggttc ctccaatgcg gtcacgatga cattcgatcg agggtcgcta
     5221 caccaccgat tctgaccgtt ttaccaatct ttttctcggt atcggctatg cctgggtagg
     5281 gaaatacggc tgagcgtcac tcctattccg agagaaggtt gttcggtcgg tcggttcgtc
     5341 gatggcaaac acttgaagac tgtcagtgag cgcgcgacgc agctggagga gaaacatggg
     5401 ggaaggaact gagttggttg ggtacgcgac attcctcttc tttgcgtaca tctatctgcc
     5461 ttacgccttc ctgaagttcg gtgccgagca atctatcgac ctcggcaaac gacgcgattt
     5521 cacgcagttc gaagagatca tttcaggatt cgttccggcg ttcgttctca acctcctggc
     5581 ttccctcctt taccttggcg taacgcgctg cagatatagg ctcgacttct ctgtgatggc
     5641 ttcgatgttt ggcagcaacc gaggcgccgt atcagactac atctacgcag gcgattgggc
     5701 aggctgcctc atctaccttt tcgtcctttg gggtgtagcg tacgctaacg gctattggtt
     5761 cggcgctgca gtgcatgaga tcgccgagac gaatccgctc gcacttcatg gcatcgagcg
     5821 cgtctgggag gaatgggaga cacacaggcg caaagtgcca gccaccgcaa agcgcaaccc
     5881 ttggactcga ttccggtctg gcttcctctg gcttggctgg tggttgtggt atcggttctt
     5941 tcaggagagt atcgttccgc tctacacttg gattgcacaa cacccgattg tgctcgtgga
     6001 aacggctcgc tatgaccgtt ttcttggccg tttcatccgc tacgagaaaa ctaccgacgg
     6061 acagttcgac gccatacggc tggaggtggc gagcaggctg ccttctcgcc gacgggcgag
     6121 ttccaacaga aaaaagacca cgcctgagga tagcaagtgc gtgccaatcg gcatgtttta
     6181 tttgaagtgg tcggagatcg caagcattgt cgtcatcggg ccagcagata gcgagaattg
     6241 cgagacagcc attgccctat tcacttggtg gacgaggaat accacctcta tggactccgt
     6301 caccccagag tcaggcggcg acgcgtgaaa gcagtatgca tagtgctcgc cgctcttgtg
     6361 gcttccgtcc taactgcgca aaccgcaccg tgcatttcgg acgacgtcct tcgccagtgc
     6421 ctgccggacc actgtctcga agaaaagtat tgtccagcgc gtggttgcgc acagagcagc
     6481 acgccggagg cggaggcgaa tcaggtaaga aagacgctgc cgccccgcgg cagtccgttg
     6541 accgacctcg agctcgagga ctttgagtcg cttcagatcc aagccaacaa actaggcatt
     6601 cagggctttg ccatcgccgt cggtaagcgc ggccgctatc gtaggtttga tctgcccccg
     6661 gcgaagacgg ggaaggcacg aggttccgtc ggcgagggga atcgtgttcg cctcaaggga
     6721 tacatcgtcg gcgtaccgca cgcccggcgc accgacagtg caaattgcag gttggtcgga
     6781 cgcgccaaca acaactacgt gatccatatc gctttcctgg cagacgacac ggagttcgac
     6841 agcgtcgtcg ccgagatgat cgcgcagaga cgggatctga actggaactt ggagaagctg
     6901 cgcaagatcg ccaccgacaa gcgcccagtc aagattaccg gacagttgtt ctacgacaat
     6961 aaacatctag tcaatagcga ccccgacgag gaacttcccg gccatccgaa acgcctatca
     7021 ttgtgggaga tccacccggt cacagagctg gaagtctgca acgaataccc tgaaaagctc
     7081 gatcaatgtt tcgagaaagg cgccatatgg acaccgctgg cgaaatgggt atcgaaacag
     7141 tgatgacgcc acgcgcgcgg tgcgcattgc tgttcgctgg catgctgcta tgcagtaaag
     7201 aagccctggc ttcctcacta gtaacgccgg tggactgtgc acccagtggc gtcaaggttc
     7261 atgcaggtcc aagtttgaac gcacccagac tcgccaaact cccgttcgga cagcgactgc
     7321 gaagcctagg gagaccgcaa gacggctggt atgaggtcgt tctgcgcaac ggacggcacg
     7381 gcttttcatg tggcgaactc ctccatgaag tgccggcgaa cgatatcgaa ttcacagtac
     7441 gtttcatgga tgttccgggc ggcgaggcaa cgatcataaa cgttggcgat accgatatcc
     7501 tgatcgatgg cggcagcgac cccgactcgc ttctgcgcta cttgcatgag ggacacctca
     7561 ttcgaggtcc gcttgagctg gcgatcgtga cggaccaaga tagtgatcac tggaacggct
     7621 tccgatgcct gcgcggcctc agcggcaaat tgccattcag gattctggag ctctgggttc
     7681 ccgatcaaga taattggtgt tcgcccgccg atgctgaaga gcttggagtc atctcaagtt
     7741 cgcttccagg gacctgcgtt ctcaagcact cggttgtcgt ggggaatgcc aagcctcctt
     7801 caaactgcaa ggtgcgcgag atttcttacc atcctaggaa ccctaccgca gtggacgtga
     7861 agttgccttt cgcacccgag gtcgatctca cgattctgcg tcccttcgag agtgcggaga
     7921 acgattgtgc atctcggagc aatgactcct ctctcgtttt catcgccaca attggtgcgt
     7981 cccgctttct attcaccagc gatcagagag ggaagaaaga gggcgatgcg aacagacagg
     8041 aggtgcaata cggcgagaag cagttgatcg agatggcgaa ccaggaaccg ggtctactca
     8101 ccgcaaacgt tctcaaggtt gctaaccatg gggccgacac gtccacaatg ccgggattca
     8161 tcagccgcgt tttcccaaga gactctacag ggccaaagtt cgctgtgatc tgttcggcaa
     8221 atcacacgaa acaggacgtt gtggtcaagc ggtgccagag gcgggctttc gttctcagca
     8281 ccgacgagcg actcggagcg caaaagggcg atgttgtctg catcaaaagg aagggagaga
     8341 aaagtctgga ttgtgcgcac gagccacgcc tctatcgtct cccgccgatc gacgttcctc
     8401 atccatcgaa taagaacccg acgatggaat gtgccccatg tcaagcgaaa acatccggac
     8461 accagaatcg gtgacacgcc cttcctctcc aactccgccc accctgcgta gagggatcga
     8521 cccgaatctt gggcggactc caactcggcg aagctgcttc ggggtgggtg gtggtgctgg
     8581 tttcgtcttg cgcggacgac cggagcgccc gcgggcggac tgcacggcgc tggcttcgag
     8641 gatctcgtcc gggacggagc ggatcgcacg catcatcttg atgccttcgg ccggcgccgg
     8701 cacgaagtcg ggcaggatct gatcgacgcc gcgaagctgg gccatcggct gctttgacaa
     8761 tcccgtcgag cgccgcaatg tccggggttc cggacgggat caacaaggcg catgcgcagc
     8821 gcgcgtcgga tcgcgctgcg ttgcgcgacg ccgtcgatcg cgaatattct cgtagccggc
     8881 tgatggcgaa gcgacccggg cgggtggaca ccccgtgtag cagcgctaca ggagccccct
     8941 acccgccgtg tgccactggg acaccgaccg tgttccatcg gcaccccgcg aggtctgcgc
     9001 cgtgcggcgc gtgcgcactc tcccgtgtcg cacgctgcgc gctggatgca cacgtccgaa
     9061 cgcggcgcgg ggcaatgctc cctctccgcg tatcaatgct gcgctctcca cgtagcaacg
     9121 ctgtccggtc gcatagcaat gctgcctctc cgcatagcaa tgctagctct ccgcgtagca
     9181 atgctccgcc tccgcatagc agtgctgcct ctccgcgtag caatgccgtc ccctccgcgt
     9241 gggaaccctc cgccgtccgc gtaggaaccc tttgcctgcg tgtagcagtg ctgcgccact
     9301 ttgtagcaat gatccacctc tgcatagcaa tgctgccaac ccggaaagca atgctgcgcc
     9361 tcggcatagc attgctccac gctccgcgca gcagcgctac gccgcccggg tggcaacgct
     9421 gcgtccccgc gttgcaacgc tttgcccgag cgcagcaacg ctacgccaca gcgcggcaat
     9481 gcttcactct ccgccaagga acgttacgca ctcggcgcgc tcttgccgcg catgttgctg
     9541 caagcgcgac acttcgggat gtcctcgcgt tgccccttga caagtcgtct cgagaagatt
     9601 gtcgaccctt gcgggttcgc aggcgcgtcc gggtttcatg acggtgacca agaacgacaa
     9661 cgcatgacct tagagccgcg tcacgcgaag gcgcgaagac cgcgaagagg catgaaccac
     9721 agagacacgg aggactcaga gttgaaacct actccgcgac ctctgcggct ctgtggtgcg
     9781 aagggctcgc tcgcgcgttg ggctgggtca ctccccgccc ctcttccctt ccagctcggc
     9841 ccaccttgcg tataggcggt cgactgtgtc ctgggcggtg tcgagggcgg tgacgcgttg
     9901 ttggaggatg gtggcgtcgg tggcgaccgt ggggtcttcg actgcgaccc tcgcagcgtc
     9961 gaggcgttcc tcggcggcgt ggacgttggc ttccatcgcg tcccattcgc gttgttcggc
    10021 ataggttagt ttttttgcct ttggcttcga aggcctcgcg tcgcgggtgg gggcggcggg
    10081 gcgggggcgg ttttcctgcc attgggtgta gtcggcgaag tattcggcgc cgcctttgcc
    10141 gtccagggcg aggatctgcg tcgagacgcg gtcgatgagg tagcggtcgt gggtgaccag
    10201 gactaacgcg ccggggaagt cgaggaggga gtcttccaac acttctaatg ttgagatgtc
    10261 gagatcgttg gtgggctcgt cgaggaccag gaggtcggcg ggttggagca tcagtctggc
    10321 caggacgatt ctcgcttttt cgccgccgga gagcttggag actgaggttt cgagctggtc
    10381 gggttggaag aggaagcgct tggcccacga tgcgacgtgc agggtgcggt cgcggtagac
    10441 gacttgatcg ccttcgggag ccagggcgcg tttcaaggtc agcgtcgggt cgaggctctc
    10501 gcggttttgt tcgaagtaga cgatgcggag tttttcggct ttctcgatgg tgccggcgtc
    10561 gggttgcagc tcgccggtga tcgttcggag gagcgtcgtc ttgccggagc cgttgggacc
    10621 gaggatgccg aggcgggagc cgggggtgag gaggatgtcg agatcggcga cgaggagttt
    10681 gtcgcccatc gacttgcgca ggcctttgac gagccagagg cgcttggttc tgcgttccga
    10741 ggcggtgaag tcgatcttgg cggtgctggt gttgctgcgc gacctcgaat ccgccagctc
    10801 cccaatcaac ccctcggcgt tttgaatccg cgatttcgat ttcgtggtgc gcgctttcgg
    10861 gccgcggcgc agccattcca cttcgcgccg cacgaggttg gcgagcgtct cctgatacgc
    10921 ggcttcgttg cgcagtagct cgtcgcggcg ctcgaggaag tcggaatagt ggccgttgac
    10981 ctggagcagg ccgtcggcgt actggcgatt gagctccagc atgcggcgcg cggtgttctc
    11041 gaggaagtag cggtcgtgac tgacgacgac gaatgctttc ggttcggact tgagcagacc
    11101 ttcgagccag aggatggcgt cgacgtcgag atggttggtc ggctcgtcga gcagcatcac
    11161 atcgggttcg cgcgcaagct cgcgggcgat ggccaggcgc ttgcgccagc cgccgctgag
    11221 cgtggcggtg tcctgggtgc ggtccatgaa accggacttg ccgagggcga tggcgacgcg
    11281 ggtttgcttg ccgtagtcgt cgaggatgtg atcgttctcg agtccttcga ggaggacgtc
    11341 ttcgatcgtc ttgccatgcg caaagacggg atcctgcggg acgtagccga tgcggacgcc
    11401 tttgcggacg gcgcggttgc cggaatcggc ttcctcgacg ccggcgacga tcttcatcag
    11461 cgtcgatttg ccggagccgt tggggccgac gaggccgacg tggtcgcctt cgaagaggga
    11521 gaaggagagg tcgttgaaga gggggcggga ggtgtaggac tttgttagtc gttcgcaggt
    11581 caggaggacg ggtggcatta gaagcattca accacagaga catagcgcgg cgtagccgca
    11641 accaaaagta aaggcgcttc gaacctctcg taggatgttg agctgacgaa agcaaaagca
    11701 tccaggaaag gaagaagcgc catgcatgag tttatcgcga agcaccggaa caagatcgcg
    11761 gggatattgt ctgggttcga ccggctggtc ttccgaggga cgttacggtc gctgagcttc
    11821 gtggacggca tgcggcacta ccttggtgcc aacgatgtat tgctgaagga cttcggtccg
    11881 cacgtggagc gggtgagcca acagttgaag gatgcttcgc ggggcctggt gaagcagctg
    11941 aaccggccgc tcaaccatct gccctcgagt aagttaagca aggagaagat tgcccgagaa
    12001 gtcgtggcca gggacggcat ccgcgagggt ctgatctgtg tgttgacttg cgtggaaccc
    12061 tgctggagtt ttgaggttca ccgcaatcgg gagaccaaga agctggagtt ggagtcgcga
    12121 taccggaagt gcgagttcat ctatcagtac tggatgcatc cgaagttcgg cttcatgaac
    12181 gcgcggattc agacctggtt tccgtttcag atccagatct gcatgaatgg ccgcatgtgg
    12241 ctagggcggc agatggatca ggcggggatc cagtacgcgg cggcagacaa ctgctttcct
    12301 tggatcgaag actggaccaa ggcccaggca gtgatggacc agcagctgag caccgattgg
    12361 ccgaaggctc tggaaccgat tgcccagcaa ctaaaccctg tccacggtga gatgttcagg
    12421 aagtatcccg tttcttacta ctggtcgacg tatcaaagcg aatgggcgac cgacatcgtg
    12481 tttcagaagg cgaaagagtt gcgacggctg tttccgcggc tggtccacca tggcatcacc
    12541 acgttttcct gtacggacgt gatgcgctac ctggggaaac caactctgtt gagtggcgag
    12601 gtgcccagga ggttctcggg agacgtggtg agcgacatca aacacaggga agaaggggtg
    12661 cggctcaaac acaccatcaa cggcaactcg gtaaagctgt acgacaaggc cttcaccaaa
    12721 ccgggcagtg tgcttcgggc cgagaccacc atccagaatg gcagcgactt ccaggtctat
    12781 cggcccaagg aaggtgaccc cgatggaaaa atgagc
//
LOCUS       DATLVW010000530        16215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_105735, whole genome shotgun sequence.
ACCESSION   DATLVW010000530 DATLVW010000000
VERSION     DATLVW010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105735"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4323)
                     /locus_tag="VNN25_15815"
     CDS             complement(<1..4323)
                     /locus_tag="VNN25_15815"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH93049.1"
                     /translation="MKRFFVSLMVALVVLPGFAAPAKKKKVKPAGDQEVLVALKTEDR
                     KPVPQDKARKTMGADDDPDATRTSGPVAVGPAVQTRMSKASSNKLDLRSLPFVAPDKV
                     ERPEREAPPSNPTAAGAHIIGGPPSTLFPQPPQRSAPAPAPLTTFDGLDFATWGAGHP
                     PDTNGDVGPTYYIQTINTSIGVFRKSDGVRVAAFTFNTFMSQGHFGNLCDTNNFGDPV
                     VLYDSFEDRWIITDFAFQLSSGNVVNPPGNFQCFAVSMNGDPVTGGWNFYSINTTGGL
                     GDYPKLGIWPDGLYMSVNMFGYPSGAAFQNTRLYALNKAQMYAGSPTVKVVSFDLPGD
                     QFTVIPANARLQTGTPPTGSPNYFASVWNFLDSVQIWKFHVDWDHLPLSTVTGASDAT
                     AASWWEQYNRSGTTTAPSPANFLDTLYPRLMVQNQYSKFGPAESLWLSHTVGAGNPGP
                     ANVTSAQAAVRYYQVDVTGGTVATSTPQAFTFSPDATVFRYMPSVAVNRAGDLAMGYT
                     TSNATTNPTLSYAGRLAGDAPNTITLTEQLLFAGTGSQSGTCGTTCTRWGDYSAMTLD
                     PDGCKFWYTNEYYGVTGLNHQTRIGSFVYPSCTPVGSGGSVQGTVTAAVGGAPISGAT
                     VNLGARTTTTTASGGYTFSGIPAGTYPTITASYVGYNPSTANSIVVTDGGTTTQNFSL
                     TSASNGSCLVDTTQTDFQSGVATNVEVTSSPGDVKLTNPNLVDQQSVTSIAGGFGLTS
                     TGWNGQTFKAGVSGTLVAVDVNLFCLTCTGTTPNFTASIRATTAGLPTGADLVSTTIA
                     GFNDGGAGSTHTADFSATPFTLTAGTTYAFIIRPVANPSAGGYDFSVSFTPNPYAFGS
                     RISSTNSGATWAATTTRSAIFHTYVNNGFSPSGNLISSVKDGNPSIGNSPTWSTLSWN
                     AVTPANTSVQFQAAGASSPNGPFNFVGPDGTAGTFFTTSGASIAQFNGSRYLEYKAIL
                     SSTNNTITPALNDVTVCFADVACGAPAPTITPTPASLCPLSTGNTASVPAGAMNYSWA
                     ATNGTITAGGSSPTVTYTAGPFGSVGLQLTETDATGCIVVSAASVPIAPGPPTITPGG
                     PTTFCSGGSVTLSSSALTGNQWSLNGNPIGGEINQTYVATATGNYSVTTTSGSCTSVP
                     STPAAVTVNPLPATPTISGTLVFCAGGNTTLTSSSATGNQWFNGGNPIGGATSQNYLA
                     TAAGNYTVQVTDGNGCVSAMSAIATVTVNPLPATPTATPGGPTTFCAGGSVTLTSSAA
                     SGNQWLLNGNPIGGATNTTYAATASGSYTVTTTDGNSCTSAPSAAVVVTVNPIPATPT
                     ITPGGPTTFCAGGSVTLTSSAASGNQWLLNGNPIGGATNTTYVATASGNYTVTTTDGN
                     SCTSAASAATTVTVNPLPATPTATPGGPTTFCAGGSVTLTSSAASGNQWLLNGNPISG
                     ATNTT"
     gene            complement(4467..4564)
                     /locus_tag="VNN25_15820"
     tRNA            complement(4467..4564)
                     /locus_tag="VNN25_15820"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4528..4530),aa:Sec,seq:tca)
     gene            complement(4684..5154)
                     /locus_tag="VNN25_15825"
     CDS             complement(4684..5154)
                     /locus_tag="VNN25_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH93050.1"
                     /translation="MIDKLVRWYDVVMSRGFDKLRSPLLLVLRLMFGWELFQTGKGKL
                     QNIDRFTEFIAHLHIPMPGVTAHFVASLECVGGLLLLLGLFARLISIPLTINFIVAYV
                     TADHEALVGFFKDQDAFTSAAPFLYLLVSLIILAFGPGFFSVDEVIRRMRRRKA"
     gene            complement(5154..5936)
                     /locus_tag="VNN25_15830"
     CDS             complement(5154..5936)
                     /locus_tag="VNN25_15830"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXH93051.1"
                     /translation="MQAVVTHPGDVYEAAEAAAIGVDSVVLPSKTLQPIQRVGIYHGM
                     YMLRMIEALEADYEALVYMMGGHAFGHLVREYVQHFPSRSYTLNRLGDHLPEFIASST
                     LKRKTFLADLARLELAMTHVFEEAEAAPMAPDAIAAVPPERIESLRFTPIPALRLLTF
                     ASNANDVFQAFREERPAEPRRGKSWLAVHRRDYGVHRMPLSQEAFTFLGFLVSGETIG
                     SAIMKFARRFRRMPEQQDLFNWFRDWSEAGLFAAIAITEQET"
     gene            complement(6181..7116)
                     /locus_tag="VNN25_15835"
     CDS             complement(6181..7116)
                     /locus_tag="VNN25_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXH93052.1"
                     /translation="MNRFNFPDLGVGIGLRTVHYAHILDAQPDIDWFEALSENYLDTG
                     GRPRYVLETIAERYPIVLHGVSMSIGGTDPIDFDYLRKLKELARGVNARWISDHLCWT
                     GVSGRNVHDLLPMPYNEESLRHTAARVRVVSDFLETPLVVENPSSYVEFATSTMTEWD
                     FLAALAAESDCGLLLDVNNIYVSSFNHGFDPNTYVDAIPADRVVQIHLAGHANHGTHI
                     IDTHDNFVIDEVWQLYGRACARTGNVATLMEWDSDIPDFETVHAEARKAEAFRGSGES
                     GVGGRRKSKTKGKTSSLRHPTPDTRPPQDVEAVYV"
     gene            complement(7260..7622)
                     /locus_tag="VNN25_15840"
     CDS             complement(7260..7622)
                     /locus_tag="VNN25_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93053.1"
                     /translation="MNSRDLARYLVLGGVIAGLSAGTSLRADQARSNTLSLNQSSQAV
                     QMADKAKAADKHACKGLNGCKGLGADGKNACKGQGSCASKEYKHSCAGQNACKGQGAG
                     GKNECKGKGSCAVPPKAS"
     gene            7984..9453
                     /locus_tag="VNN25_15845"
     CDS             7984..9453
                     /locus_tag="VNN25_15845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HXH93054.1"
                     /translation="MDRRTNPILIWIAALGSIAVVTAGGSALRLNPNTVGFAYLIVVL
                     LISVWGGLLAGTVSSVIATLCYNFFFFPPLYTFTIENPANWFALVAFLVASVTVNRLV
                     VAAHVQAEKAKQRRTELETLYGLSIDLFAATNRVGALGEAAGRALQLLGARAGGLVLF
                     DGSPFRQNIVSWTGDKPDEIEDLIAGVGRHKESLEFPSPLGRDVYLPLIVGGKTTGAL
                     VARGTEASKQTLESAARLVALAVERERFIEENAHVRALRESEALKTSLLRAISHDLTT
                     PITAVSIRTESIRRRAGADPELLEDIGAIAEETARLRRRIDNLLAMARLEAGKSKPHR
                     EPTPPADLFRATRENLPIVFSARPITVHVEPDCPDANVDPSLALEILVNLVENAHRVS
                     PPNATIELVARKHPIDADKMRIEILDRGPGLPPGVVDADGNVATETTDLAQRGLGLEI
                     ARGLAAANGGSIGLAPRAGGGTIARVDLPAASLPAEERA"
     gene            9450..10136
                     /locus_tag="VNN25_15850"
     CDS             9450..10136
                     /locus_tag="VNN25_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH93055.1"
                     /translation="MTISNVILIVDDDPAIRDSLSKELRANGYSTTTAADGAEGMSAF
                     QSQVPDLVLTDLAMPRSDGFELIAGIRASSRVPVIVLSVRGADPDKVRALDLGADDFV
                     TKPFSMAELLARVRAQLRRTAPPSTTLNFPDLTIELDRRRVVQGGRELRLTPTEYSLL
                     ELLATNAGKPMFIEQIIARVWRSAPSTSADTLRVHMSSLRKKLEPNPSEPLYIVTEPW
                     VGYRFIAEPI"
     gene            10352..10696
                     /locus_tag="VNN25_15855"
     CDS             10352..10696
                     /locus_tag="VNN25_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93056.1"
                     /translation="MAVVFWSLAALLVALAHQRLDATSPLASAAIEVGTIVAVAAVYI
                     RIVSPESTLDHALFVGTTWVLLAISAEIIMTASSGRQWFALLGSPAHGGLRCVLLIAW
                     LIAPALFVRHHA"
     gene            10707..12704
                     /locus_tag="VNN25_15860"
     CDS             10707..12704
                     /locus_tag="VNN25_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXH93057.1"
                     /translation="MNDQPPEQSTIQRVRRMIVGPPRDVSDPKIFHHVSLIAFLAWVG
                     LGADGLSSSAYGPEEAYKALGPHSHLSIILVAMTAVTIAVISIAYSNLIQHFPGGGGG
                     YLVATKLLGSKVGVVSGCALLVDYVLTITVSIASGCDQLWSFLPLSAAPYKLLTEFLI
                     LFILIVLNLRGVKESVNILAPIFVLFIVTHGFAIVYAIASHATGLPKVFHDAHVDYSH
                     SVSTIGFLPLMLILLRAYSLGGGTYTGIEAVSNGVSMLREPRVRTGKRTMALMAASLA
                     FTAGGIMFAYLLTNSTPAEGKTMNAVLLTNLFGRWHIGTLSAGTGFVILCLVAEAALL
                     FVAAQAGFLDGPRVLSNMALDSWMPHRFSQLSDRLVTKNGVYMMGIAAAAALLYTRGD
                     ILTLVVMYSINVFITFSLTELGMARHWIKDRDKEPKWKSQVAIHGTGLLMCLSILVIT
                     VFEKFSHGGWVTVVITGLVIALCFAIRRHYDEVRAGLKRLDDILTTVPLPETGTATGP
                     SELDPKLPTAVISISSFSGFGLHQILSIHKAFPNYFKQLVFVSAAVVDSGNFKGAEEL
                     GRLQTNTEANLLKYVAWAQGHGFRADYRMALGTEAVATVEAICLEVAKEFPRAIFFTG
                     RLIFKEEKWYHRLLHNETPHAIQRRLQFEGIQAMVLPIRVL"
     gene            complement(12736..13899)
                     /locus_tag="VNN25_15865"
     CDS             complement(12736..13899)
                     /locus_tag="VNN25_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93058.1"
                     /translation="MRIAPVVALLPLLMAHCAVEPRHDYRADYNQARASLASRKRGAP
                     GLGVALNRAWSAVANWTAGFLDEHPDATAEQLAKAIIALEPRRPCSQDDDEHCFHLDA
                     TAVLLNGPPATTFAVAANYPKSGTFFVVARARDGHFGAMWNIKDVALRHYASRDEIGY
                     WAWTGGGWGDGPPTSIIGSLPRSASGHPRFYVDAVAAAEAGGTYRKQFSVWEWDGVEA
                     RPLFIKSYSVSFETAPVTVKDGNVSIPAKGEYKSFFTCGMCPEPHVLRTLRVSPDGVH
                     DLGTSDGEPEMRCVDELWDRLIHGQPAAGLASPEVVAALRPIVEDVKHPAGSDLSNSL
                     GMIEIPKVSNEDGRRFLLIASEDLDCRQLRFEIGSAASGACLRTLRVLNPCAK"
     gene            14137..14553
                     /locus_tag="VNN25_15870"
     CDS             14137..14553
                     /locus_tag="VNN25_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93059.1"
                     /translation="MISMTQSIDRQAAQRKHNAATRAAVIVVFWGVAALLVAVANRSM
                     YATSPVICVAIEVVFIVAVAGMYVRLVMPASTLDHALLVGTTWVLLGIATEIAMTANQ
                     GRQWTALIGPPANGGLRCVILIAWIIAPALFVRSRE"
     gene            14550..15605
                     /locus_tag="VNN25_15875"
     CDS             14550..15605
                     /locus_tag="VNN25_15875"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXH93060.1"
                     /translation="MRTRSFCVLAFAVLLIAVVARAEDPPKPVTIHGFVDGYYAWNSN
                     DPASHDSFFPGTGSTAERANELRLNLAAVKIVRDAAPLGFHLSLVAGDGADVIHAAES
                     KGFRHIYQASVIYKVSDKLTIEGGIYPSHIGFESFYSKDDWNYMRSWPGELSPFYQTG
                     VHATYQVDSHWSGDVHVLNGWQHIKDNNDAKAIGVKITYTSDQLATSLNTFDGPELPH
                     DNTHWRHFGDLIASYKATAKLSLAGSIDRGHQELPGDAAANWLGIAGFARYTFDDRHA
                     VAVRGSLFHDPDNAVSGAAQKLTEETLTYEFHPVTNLIVKIEGRHDHSTAAVFDRKTN
                     ESTNNQTIAVIGAVVTF"
     gene            15707..15796
                     /locus_tag="VNN25_15880"
     CDS             15707..15796
                     /locus_tag="VNN25_15880"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HXH93061.1"
                     /translation="MTFEAIVGLVLAVFALIYLLYALVRAEDL"
     gene            15796..>16215
                     /locus_tag="VNN25_15885"
     CDS             15796..>16215
                     /locus_tag="VNN25_15885"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXH93062.1"
                     /translation="MTTLELLKIAIYFLLILTVTKPLGLYMRRVFCGERTFLDPLLRP
                     VERFVYRAGGVDEKREHDWMQYTIAMLLFNSVGLLFTYAILRLQHLLPLNPDKLGAVP
                     PDLAWNTAVSFTTNTNWQAYSGESAMSHLSQMVALTFH"
BASE COUNT         2909 a         4988 c         5101 g         3217 t
ORIGIN      
        1 cgtggtgttc gtcgcgccgc tgatggggtt gccgttgaga agccactggt taccgcttgc
       61 cgcgctggaa gtcagcgtga cgctgccgcc ggcgcagaag gtggtcggtc cgcccggtgt
      121 cgccgttggc gtcgctggga gcggattgac ggtgacggtc gtcgcggccg atgcggcgct
      181 ggtgcagcta tttccgtcgg tggtcgtgac cgtgtagttg cccgaagcgg tggcgacata
      241 agtcgtattc gtcgcgccgc cgatgggatt gccgttgaga agccactgat taccgctcgc
      301 ggcgctggag gtcagcgtaa cgctgccacc agcgcagaac gtagtcggtc cgccgggggt
      361 gatcgtcggt gttgctggaa tcggattgac agtgacaacg acggcggccg acggcgcgct
      421 ggtgcaggag ttgccgtcgg tggtcgttac cgtgtagctg cccgaggcgg tggcagcgta
      481 cgtggtgttc gtcgcgccgc cgatggggtt gccgttgaga agccactggt tgccgcttgc
      541 ggcgctggaa gtcagcgtca cgctgccgcc tgcacagaag gttgtcggtc cgcccggggt
      601 cgccgtaggc gtcgcgggca gcggattaac cgtgaccgtg gcgattgcgg acattgcgct
      661 gacgcagccg ttgccgtcgg tgacctgaac ggtgtaattg cccgcagcgg tggcgaggta
      721 attctgactg gtcgctcccc cgatcgggtt gccgccgttg aaccattggt taccggtggc
      781 gctgctcgaa gtcagcgttg tgttgccgcc ggcgcagaag acgagcgtgc cgctgatcgt
      841 gggcgttgcc ggcagaggat tgacagtgac ggcggccggc gtcgacggga cgctggtgca
      901 ggagccggat gtcgtcgtga ctgagtagtt gccggtagcc gtggcgacat acgtctggtt
      961 gatttccccg ccgatcgggt tgccgttgag ggaccactga ttgcctgtga gcgcgctcga
     1021 gctcagggtg acgctgccgc cactgcagaa cgtcgtcggt ccgccggggg tgatggtggg
     1081 cgggccgggt gcgatcggga cgctggccgc gctcacgacg atgcatccgg tggcgtcggt
     1141 ctcggtcaac tgcagcccga ccgagccgaa gggcccggcg gtataggtca cggtcggcga
     1201 ggatccgccg gccgtgatcg tgccgttggt ggccgcccat gaataattca tggcgcctgc
     1261 cggtaccgaa gcggtgttgc cggtcgagag gggacagagc gacgcgggag ttggcgtgat
     1321 cgtcggggcg ggcgcgccgc aggcgacgtc ggcgaagcaa accgtaacgt cattcagggc
     1381 gggcgttatg gtgttgttcg tcgatgagag gatggccttg tactccagat agcgggagcc
     1441 gttgaactgc gcgatcgacg cacccgaggt cgtgaagaac gtgccggcgg ttccgtccgg
     1501 accgacgaag ttgaacgggc cgttgggact gctggcgccg gcggcctgga actgcactga
     1561 cgtgttcgcc ggagtaacgg cgttccatga cagcgtcgac cacgtcggcg aattgccgat
     1621 cgacggattg ccgtccttca ccgaagagat gaggttgccg gacggcgaga agccattgtt
     1681 cacgtaggtg tgaaagattg ccgagcgcgt cgtcgtagcg gcccaggtgg caccgctatt
     1741 ggtggacgag atgcggctgc caaaggcata gggattcggt gtgaaactga cgctgaagtc
     1801 atagccgccg gccgatgggt tggcgacggg acggatgatg aacgcgtagg tggtgcctgc
     1861 ggtgagagtg aacggtgtgg cgctgaaatc ggcggtgtgc gtactgccgg ctcctccgtc
     1921 gttgaagccg gctatcgtcg tcgataccag atccgctccg gtcggaaggc ccgcggtcgt
     1981 ggcccggatg gatgcagtga agttcggcgt ggtgccggtg caggtcagac agaagagatt
     2041 gacgtcgacg gccacaagcg tgccgctgac gccggctttg aaagtctggc cgttccagcc
     2101 cgtagacgtg aggccgaagc cgcctgcgat cgacgtgacg ctctgctggt cgacgaggtt
     2161 cggattcgtt agcttgacgt cgccgggact ggaggtcact tccacgttcg tggcgacgcc
     2221 gctctggaag tccgtctgcg tcgtgtcgac gaggcagcta ccattgcttg cggacgtgag
     2281 cgagaagttt tgcgtcgtcg tgccgccgtc ggtgaccacg atggagttgg ctgtcgaggg
     2341 gttgtagcca acatagctgg cggtaatcgt gggatacgtt cccgccggaa tgccggagaa
     2401 ggtgtagccg ccggacgcag tggtcgttgt cgtccgtgcg ccgagattca ccgtcgcgcc
     2461 gctgatcggc gcgcctccaa ctgctgcggt gaccgttccc tgcacgctgc cgcccgatcc
     2521 cacgggagta cacgacgggt aaacgaacga gccgatgcgg gtttgatgat tgaggccggt
     2581 gacgccgtag tactcgttcg tgtaccagaa cttgcagccg tccggatcga gcgtcatggc
     2641 gctgtagtcg ccccagcggg tacaggtggt gccgcaggtg ccgctttgcg agccggttcc
     2701 agcgaagagc agttgctcgg tgagagtgat ggtgttcggc gcatcaccgg ccagacggcc
     2761 ggcataactg agcgtgggat tggtcgtggc gttcgatgtc gtgtatccca tcgccaggtc
     2821 accggctcgg ttgaccgcta cgctgggcat gtaacggaag accgttgcat cagggctaaa
     2881 cgtgaaggcc tgcggcgtcg aggtggcgac cgtaccgccc gtgacatcga cctggtaata
     2941 gcgaacggcg gcctgggccg acgtgacgtt cgccggcccg ggatttccgg cgccgacggt
     3001 atggctcagc cacaacgatt ccgcggggcc gaacttcgaa tattggttct gcaccatgag
     3061 ccgggggtag agcgtgtcga ggaagtttgc gggactgggc gcggtcgtag taccgctccg
     3121 gttgtactgc tcccaccacg atgccgcggt ggcatcggag gcaccggtga cggtagagag
     3181 cggaagatga tcccaatcga catgaaactt ccagatctgg actgagtcca gaaagttcca
     3241 gaccgaggcg aagtagttcg gacttcctgt gggcggcgtg ccggtctgca atcgcgcgtt
     3301 cgccggaatg actgtgaact gatctcccgg caggtcgaat gaaacgacct tcacggtcgg
     3361 gctgcccgca tacatctgcg ccttgttcag cgcgtacaac ctcgtgttct ggaacgccgc
     3421 tccggatggg tagccgaaca tgttcaccga catgtagaga ccgtccggcc agatgccgag
     3481 cttcgggtaa tcgccgaggc cgccggtggt gttgattgag taaaagttcc atccgccggt
     3541 gaccggatcg ccgttcatcg agacggcgaa gcactggaag ttgcccggtg ggttgacgac
     3601 gttgccgctc gaaagctgaa acgcaaagtc ggtgatgatc cagcggtctt cgaagctgtc
     3661 gtaaaggacg acgggatcac cgaagttgtt cgtgtcgcag agattcccga agtgtccctg
     3721 actcatgaag gtgttgaagg tgaaggcggc cacgcgcacg ccgtcggact tccggaaaac
     3781 gccgatcgag gtgttaatgg tctggatgta ataggtaggt cccacatcgc cgttcgtatc
     3841 aggaggatgg ccggcgcccc aggtggcgaa atcgagaccg tcgaaggtcg tgagaggggc
     3901 aggtgccggc gcgcttctct gcggcggctg cgggaagaga gtcgatggcg ggcctccgat
     3961 gatatgggcg cctgctgcgg tcggattcga cggcggcgct tcgcgctcgg ggcgctcgac
     4021 cttgtccggg gctacgaacg ggagcgaacg aaggtcgagt ttgttcgacg atgctttgct
     4081 catccgcgtc tgaaccgcgg gcccgaccgc caccgggccg gaggtcctgg tggcatccgg
     4141 atcgtcgtcg gcgcccatag tcttccgggc cttgtcctgc gggaccggct tgcggtcctc
     4201 cgtcttcagg gcgacgagta cttcctgatc gccggccggc ttaacctttt tcttcttggc
     4261 cggggcggcg aaaccgggca gcacgacgag cgctaccatt agagagacga agaatcgttt
     4321 catgcgaggt cctttggttg tggggaggcc ggtggggagg ttgggcggcg tgatgatgac
     4381 agattattct ggcggttgca tcagcaactc accgtcgatg acttctatcc gccagaagat
     4441 cagcgtgcgc ggtttctcgc gccggttggc ggaggcgggt cggaatcgaa ccgacccttc
     4501 gacgcttccg cgccgaaaca acggttttga agaccgcggg agccaccaga ccccattcgc
     4561 ctcctcggga ggagaacgga cgataacacg cggtcgcggg gtttcggggc tgcgcggtct
     4621 cgcggtcgag agcgagattc agacctgatc ggacggatcc gacctatcgt acggatccac
     4681 tgatcacgcc ttgcgccggc gcattcgcct gatcacctca tccactgaga aaaaacccgg
     4741 accgaaggcg aggatgatca gcgatacgag gagatagagg aacggcgccg cgctcgtgaa
     4801 ggcatcctga tccttgaaga atccgaccag cgcttcgtga tcggcggtca catacgccac
     4861 gatgaagttg atcgtcagag gaatggagat cagacgcgcg aacagcccga ggaggagaag
     4921 cagaccgccg acgcactcga gcgaagcgac gaagtgggcg gtcacgccgg gcatcgggat
     4981 gtgcaggtgc gcgatgaact cggtgaagcg gtcgatgttc tgcagcttcc ccttccctgt
     5041 ttgaaacagc tcccagccga acatgagccg gagcacgagc aggagcggac tgcgcagctt
     5101 gtcgaatccg cgcgacatga ccacgtcgta ccaccgcacc agcttgtcga tcatcacgtc
     5161 tcctgttcgg tgatggcaat tgccgcaaaa agcccagcct cactccagtc gcggaaccag
     5221 ttgaagaggt cctgctgctc cggcatccgc cggaagcggc gcgcaaactt catgatggca
     5281 ctgccgatgg tctctccgga aacgagaaag cctagaaatg tgaaggcttc ctgcgaaagc
     5341 ggcatgcgat ggacgccgta atcgcgccgg tgaaccgcaa gccaggactt cccgcggcgc
     5401 ggctcagccg gacgctcttc acgaaacgcc tggaagacgt cattcgcgtt ggatgcaaag
     5461 gtcagaagac ggagtgccgg gattggcgtg aagcgcaacg attcgatccg ctccggcggc
     5521 acggcggcga tcgcgtccgg agccatcggc gcggcttcgg cctcttcgaa tacatgcgtc
     5581 atcgccagtt cgaggcgggc cagatcagcg aggaacgttt tcctcttcaa cgtcgagctg
     5641 gcgatgaact ccggcagatg gtcgccgagc cggttcagcg tgtagcttcg cgacggaaag
     5701 tgctgcacgt actcccgcac cagatgaccg aacgcgtgcc cgcccatcat gtatacgagc
     5761 gcttcgtagt cggcctccag cgcctcgatc atccgcagca tgtacatgcc gtgatagatc
     5821 ccgacccgct gaatcggctg cagcgttttc gacggcaaca ccaccgaatc gacgccaatc
     5881 gccgccgcct cggctgcctc atacacatcg ccaggatgcg tgaccaccgc ctgcatccac
     5941 tcctgcagcg gggcaagtgg gaggttgcgg gggtcgcgtg tcgggggtcg ggtatcggag
     6001 agacgtggtt tgcgggggtc gcgcttcggg gggcgcgtgt cggagagacg tggttttgcc
     6061 ttttctccga ctcccgacgt gctccccggg ggtcgcgtgt cgggggtcgg gggtcggaga
     6121 gatttggggt tgcttttgct ccgactcccc acccccgact ccccactccc tccgagcacg
     6181 tcagacatac acggcctcta cgtcttgcgg gggtcgcgtg tcaggcgtcg ggtgtcggag
     6241 agagctagtc ttgcctttgg ttttgctttt tctccgaccc ccgactcccg actccccact
     6301 cccgcgaaac gcctccgcct tccgcgcctc agcatgcacg gtctcgaaat ccgggatatc
     6361 cgaatcccac tccatcagcg tcgcgacgtt gccggtgcgc gcgcaggcgc ggccgtagag
     6421 ctgccacacc tcatcgatga cgaaattgtc gtgcgtgtcg atgatgtgcg tgccgtggtt
     6481 cgcgtgtcct gccaggtgga tttgcacgac gcgatcggcg gggatggcgt cgacgtacgt
     6541 attcggatcg aagccgtggt tgaaggagct gacgtagata ttgtttacgt cgagcagcag
     6601 tccgcagtcg ctctcggctg ccagcgctgc cagaaaatcc cactccgtca tcgtcgatgt
     6661 tgcaaactcg acatacgacg acggattctc cacgaccagc ggcgtctcca ggaaatcgga
     6721 gacgacgcgc acgcgcgcgg cggtgtggcg aagcgattcc tcgttgtacg gcatcggcag
     6781 aagatcgtgg acgttgcgtc cgctgacgcc ggtccagcag aggtggtcgg agatccagcg
     6841 ggcgttgacg ccccgtgcca gctccttcag tttccggagg taatcgaaat cgatcggatc
     6901 ggtgccgccg atcgacatcg agacgccgtg aagaacgatc gggtatcgct cggcgatcgt
     6961 ctccagcacg tagcgaggac gaccgccggt gtcgagatag ttttcggaga gtgcctcgaa
     7021 ccagtcgata tcgggctgcg catcgaggat gtgcgcgtaa tgcacggtcc gcaatccgat
     7081 gccgactccg aggtcaggga agttgaatcg attcatgggt tctttggagg gggtgggggg
     7141 tgggagagag ctcgtgcgac tggccagctt actagtcgcc gaacatctct tccacatccc
     7201 accccccact tcccaccccc cgaggaggac ggccgacccg tcctccgggg agtggtggat
     7261 tacgaagcct tcggaggaac ggcgcaggag cctttgccct tgcactcgtt cttgccgccg
     7321 gcgccctgac ccttgcaggc gttctggccg gcgcagctgt gcttgtattc cttcgacgcg
     7381 cacgagccct ggcccttgca ggcattcttg ccgtcggcgc cgaggccctt gcagccgttg
     7441 aggcctttac aggcatgctt gtcggcggcc ttggccttgt cagccatctg cacggcctgc
     7501 gacgactggt tgagagaaag cgtgttcgac cttgcctggt cagcacgcag actggtaccg
     7561 gcgctgaggc cagcgatgac accaccgaga acgaggtaac gagccagatc acgactattc
     7621 atacaaatct cctcctgaaa attgtttgag taccgacaga cttacggccg acagtgggtg
     7681 attggatgcc atgatgccag attcggagaa gaatagcgct gccggagatc gcggatgggg
     7741 ttccatttct gcgatgaaac ggtgtcaaaa ctccctgcga gggaagtagg tgccgatcga
     7801 atcaaactct ctttgaacca cagagacaca gagaacacag aggacacgac aatgactctg
     7861 tgtcctctgt gtctctgtgg ttcaggggtt taaacgagat tctcgcccgt aatcaagatc
     7921 tacgccgatc tttagccctc ctttacgctc atctggtccc tcctgtgttt cactggtctg
     7981 agcatggacc ggcgaacgaa tcccatcctc atctggatcg ccgcgctcgg gtcgatcgcg
     8041 gtcgtcaccg ccggcggctc ggcgctcagg ctcaatccga acaccgtcgg cttcgcgtac
     8101 ctcatcgtcg tccttctgat ctcggtctgg ggcgggttgc tggccggaac cgtgtcgtcg
     8161 gtcatcgcca cgctttgcta caacttcttc ttctttccgc cgctctatac gttcacgatc
     8221 gagaacccgg ccaactggtt tgccctggtc gcttttctcg tcgcctcggt caccgtgaac
     8281 cgtctcgtcg tcgccgcgca cgtgcaggcc gagaaggcga agcagcggcg gacggagttg
     8341 gagacgctct acggattgag catcgacctc ttcgcggcta cgaaccgcgt cggcgccctc
     8401 ggcgaggcgg cagggcgcgc gttgcagctc ctcggcgcgc gcgcaggcgg actcgttctc
     8461 ttcgacggca gtcccttccg gcagaacatc gtctcgtgga ccggcgacaa gcctgacgag
     8521 atcgaagacc tgatcgccgg cgtcggacgc cacaaagagt cactggagtt tccgtcgcca
     8581 ctcggacgcg acgtctacct cccactcatc gtcggcggaa aaaccacggg cgcgctcgtc
     8641 gctcgcggca ccgaggcctc caagcagacg cttgaatccg cggcccggct cgtggcgctg
     8701 gcagtggaac gggaacgctt catcgaagag aacgcccacg ttcgcgccct gcgtgaaagc
     8761 gaagccctca agacctcgct gctccgggct atctcgcacg acctcaccac gccgattacc
     8821 gctgtatcga ttcggacgga gtcgatccgg cgccgcgcgg gagccgaccc cgagttgctc
     8881 gaagacatcg gtgccatcgc cgaggagact gcgaggctgc ggagacgcat cgacaacctc
     8941 ctggccatgg cgcggctcga ggcaggcaag tcgaagccgc accgcgagcc gacgccgccc
     9001 gccgatctct ttcgcgccac gcgcgagaac ctccccatcg tcttcagcgc gcggcccatc
     9061 acggttcacg tcgagccgga ctgccctgat gccaacgtcg atccctcgct ggcgctggag
     9121 attctcgtca acctcgtcga gaacgcgcat cgcgtgtcgc cgccgaatgc gacaatcgag
     9181 ctcgtggcgc gcaaacatcc catcgacgcg gacaagatgc gtatcgagat cctcgaccgc
     9241 ggtcccggcc ttccgcccgg tgtggtcgat gcggacggca acgtagccac cgagacgacc
     9301 gatctcgccc aacgcggcct cggcctggag atcgcgcgcg gccttgccgc cgcgaacggc
     9361 ggcagtatcg gtctcgcgcc gcgggcgggc ggcggcacca tcgcccgcgt cgatcttccc
     9421 gcggcgtcct tgcccgccga ggagcgcgca tgacgatctc gaacgtcatt ctcatcgtcg
     9481 atgacgatcc cgccatccgc gactcgctct cgaaggagct gcgcgccaac ggctattcga
     9541 cgaccacagc ggccgatgga gccgagggga tgagtgcctt tcagtcgcag gttcccgatc
     9601 tcgtgctcac cgatttggcc atgccgcgct cggacggatt cgagctcatc gccggaatcc
     9661 gtgcgtcgtc gcgagttccc gtcatcgtcc tctcggtccg cggcgccgat cccgacaaag
     9721 tccgcgccct cgacctcggc gccgacgact tcgtgacgaa gcccttttcg atggccgagc
     9781 tcctggcgcg cgtccgggca caactacgcc gcaccgcgcc accatcgacg acgctcaatt
     9841 tccccgactt gacgatcgag ctcgatcgcc gccgcgtcgt gcagggcgga cgcgaattgc
     9901 gtctgacgcc gaccgagtac tcccttctcg aactgctggc cacgaacgca ggcaaaccga
     9961 tgttcatcga acagatcatc gcgcgagtct ggcgctccgc ccccagcacc agcgccgaca
    10021 cgctccgcgt tcacatgagc tccctccgca agaagctcga gccgaacccg tccgagccgc
    10081 tctacatcgt gaccgagccg tgggttggct atcgcttcat tgccgaaccg atttgatccg
    10141 cagatgacgc agatgttcac agatgcgagg cttttatctg cgtccctctg cgtcatctgc
    10201 ggataagcct ttcgaaactc tttatcgact cttaagtgtt tccgctacca atccgccaat
    10261 agagtttgag accggtatgg tctcgcatgc gactcactcg gaagcgtcct ctgtccacgg
    10321 ccggaagcgt cacggcgtga cacgagtggt gatggccgtt gtcttctgga gcctggccgc
    10381 acttctcgtc gccctcgcgc atcagaggct ggatgcaact tcaccactgg caagtgccgc
    10441 tatcgaggtg ggcaccatcg tcgcggtggc ggcggtgtac atccggatcg tgtctcccga
    10501 gtcgacgctc gatcatgcgc ttttcgtcgg taccacgtgg gtacttctgg cgatctccgc
    10561 ggagatcatc atgacggcga gctccggccg gcaatggttc gcccttctgg gctcgcctgc
    10621 gcacggcggc ctgcgctgcg tactgctcat cgcgtggctc attgcgccgg cgctatttgt
    10681 cagacaccac gcctgagctc atggtcttga acgaccaacc accggagcag tcgacgattc
    10741 aacgcgtgcg gcggatgatc gtcggcccgc cgcgcgacgt gagcgatccg aagatctttc
    10801 atcacgtctc gctcatcgcg tttctggcat gggtcgggct cggcgcggat ggattgtcat
    10861 cgtcggcgta cggtcctgaa gaagcctata aagcactggg accgcactcg cacctgtcga
    10921 tcattctcgt agcgatgacg gcggtgacga tcgcggtcat ttcgatcgcg tactcgaacc
    10981 tgattcaaca ctttcccgga ggtggcggcg gatatctcgt ggccacgaag ctgctgggga
    11041 gcaaagtcgg cgtcgtgtcg ggctgcgctt tgctggtcga ctacgttctg acgatcacgg
    11101 tgtcgattgc cagcggctgc gatcagctct ggtcgttcct tccgctctcc gcggcgccct
    11161 acaaactgct caccgaattc ctcatcctct tcatcctcat cgttctgaac ctccgcggcg
    11221 tcaaggaatc ggtcaatatc ctcgcgccga tcttcgtgtt gttcatcgtc acacatggct
    11281 ttgcgatcgt gtatgcgatc gcctctcacg cgacaggcct gcccaaggtc tttcacgacg
    11341 cgcatgtcga ctactcccat tccgtcagca cgatcggatt cctgccgctc atgctcattc
    11401 tcctgcgcgc ctactcgctt ggcggcggca cgtacaccgg aatcgaggct gtctcgaacg
    11461 gcgtgtcgat gcttcgcgag ccgcgtgtcc gcacggggaa gcggaccatg gcgctcatgg
    11521 ccgcttcgct ggcgttcacg gccggcggaa tcatgtttgc ctatctcctc acgaactcga
    11581 caccggcaga gggaaagacg atgaacgccg tgctgctgac gaacctgttc ggccgctggc
    11641 acatcggaac cttatccgca ggcacggggt tcgtgatcct ctgtctcgtg gccgaggcgg
    11701 cattgctgtt cgtggccgcg caagcgggct tcctcgacgg accgcgcgtt ctcagcaata
    11761 tggcgctcga ttcctggatg ccgcaccgtt tcagccagct ctccgacagg ctcgtcacca
    11821 agaacggcgt ctacatgatg gggatcgccg ccgcggccgc gctgttgtat acccgaggag
    11881 acatcctcac gctggtggtc atgtactcca tcaacgtgtt catcaccttc tccctgaccg
    11941 agctgggaat ggcgcggcat tggatcaaag accgagacaa ggagcctaaa tggaaaagcc
    12001 aggtcgcaat ccacggaacc ggactgttga tgtgtctcag catcctggtg atcacggtat
    12061 ttgagaaatt ctcccatggc ggatgggtca ccgtggtcat tacaggactc gtgatcgccc
    12121 tctgcttcgc gatccgccgt cactatgacg aggtccgtgc gggcctgaag cggctcgacg
    12181 acattctgac gacggttccg cttcccgaga cgggaaccgc caccggccct tcggaactgg
    12241 acccgaaact gcccaccgcg gtgatcagta tctcgtcgtt ctccggattc gggctccacc
    12301 agatcctgtc gattcacaag gcctttccga attacttcaa acagttggtc ttcgtctccg
    12361 ccgcggtggt cgactccggc aatttcaaag gcgccgagga gcttggccgt ctgcaaacga
    12421 acaccgaggc caacctgttg aagtacgtgg catgggctca gggccacggt ttccgcgccg
    12481 attatcggat ggccctcggc accgaagcag tggccacggt cgaagcgatc tgtctcgagg
    12541 tcgcgaaaga gtttccgcgc gcgatcttct tcacaggacg cctgatcttc aaagaagaga
    12601 agtggtatca ccggctgctc cacaacgaaa cccctcacgc cattcagcgc cggctgcaat
    12661 tcgaaggcat tcaggcgatg gttcttccga ttcgggtgct gtaagtcctg ccgagcgctg
    12721 gagtctcgcc cttcgttact tcgcgcatgg gttgagaacg cgcaacgttc ggaggcaagc
    12781 gccgcttgcg gccgatccaa tctcgaaacg caactgcctg caatcgagat cttccgacgc
    12841 gatgagaagg aatcgacgtc catcctcatt ggagactttc gggatctcga tcatccccaa
    12901 tgagttgctc aggtccgatc cggccgggtg ttttacatcc tccacgatcg gacggagtgc
    12961 agcgaccact tcgggagagg cgaggccggc tgccggctgc ccatggatca ggcggtccca
    13021 cagttcatcg acacatcgca tctcaggctc accatcgctg gtgccgaggt cgtgcacacc
    13081 gtcgggactc acgcgaagag tgcggagaac atgtggttcc gggcacatac cgcacgtgaa
    13141 gaagcttttg tactcgccct tggccgggat ggagacgttg ccgtccttca ccgtcaccgg
    13201 tgcggtttcg aaggagaccg aatacgactt gatgaagagc ggcctggcct cgacgccgtc
    13261 ccactcccac acgctgaact gcttcctgta cgtaccgccc gcttcggccg ctgctaccgc
    13321 gtcaacataa aagcgcgggt gacctgatgc ggaacgcgga agtgaaccga tgatgctcgt
    13381 gggcggcccg tcgccccagc cgcctccggt ccaggcccaa tagccgatct catcgcgcga
    13441 cgcgtagtgc cgcagcgcaa catctttgat gttccacatc gcgccgaaat ggccgtcgcg
    13501 cgcgcgtgcg acgacgaaga aggtcccgct cttcggatag tttgcggcca cagcaaacgt
    13561 cgtcgctggc ggaccgttca agaggacagc cgttgcgtcc aggtggaaac agtgctcgtc
    13621 gtcgtcctgg gagcacggcc gccgaggttc gagcgcgatg atggctttcg ccagttgctc
    13681 cgcggtagcg tcgggatgct catcgaggaa tcccgccgtc cagttggcga cggcggacca
    13741 tgcacgattg agggcgacgc ccagccccgg cgcgcccctc tttctcgaag ccagcgacgc
    13801 gcgagcctgg ttgtaatcgg cgcgatagtc gtgacgcggc tcgaccgcgc aatgcgccat
    13861 cagcaaagga agcaaggcca cgaccggagc gatccgcatg cagtcccagc ctagcaccgg
    13921 caattgctaa agctggcaac tcttcttctt tcagccgccg aaacgatacc gcgacggaag
    13981 cgcgccccgc gcagccattg atctgattct taagaccttc ctgagccttt cgacactctt
    14041 aatccactct taactgcttt caaccgccgc gttttcgttc ttattaacaa ggtcctatcc
    14101 gaccgcgtgt tggagcgttc gtcgggaggg ccggccatga tcagcatgac gcagtcgatc
    14161 gatcgccaag ccgcgcaacg gaagcacaac gcagcgacga gggccgcggt gatcgtggtc
    14221 ttctggggtg tggcagcgct gcttgtcgct gtcgcgaatc gaagcatgta cgcgacgtcg
    14281 ccggtgatct gcgtggcaat cgaagtcgtc ttcattgtcg ccgtcgcggg aatgtacgtc
    14341 aggctggtta tgcccgcatc cacccttgac cacgcgctgc tggtcggcac cacgtgggtg
    14401 cttctcggga tcgccacaga aatcgctatg acggccaacc aggggcgaca gtggactgcc
    14461 ctcataggtc cgccggcaaa tggcggactg cggtgcgtga tcctgatcgc ctggatcatc
    14521 gctccggcgc tattcgtcag gagccgcgag tgagaacacg ttcgttttgt gttttggcct
    14581 tcgctgtcct tctcatcgcc gttgtggctc gtgcagagga cccgccaaag ccggtcacca
    14641 ttcacggatt cgtcgacggc tattacgcct ggaacagtaa cgatcccgca tcgcacgact
    14701 ccttctttcc tggcaccggc agcactgcgg agcgtgcgaa cgagctccgt ctcaatcttg
    14761 cagccgttaa aatcgtccgc gacgctgcgc cactcggctt tcatttatcg ctggttgccg
    14821 gggacggtgc ggatgtgatt catgccgcag agagcaaggg ctttcgtcat atttaccagg
    14881 cctccgtaat ctacaaagta tccgacaagc tgaccatcga aggcggcatc tatccgtcgc
    14941 acatcggctt cgaatccttc tattcgaagg acgactggaa ctacatgcgc tcgtggcccg
    15001 gtgagctttc gcccttttac cagaccggcg tccatgccac ctaccaggtc gacagccact
    15061 ggagcggcga cgttcacgtc ctcaatggat ggcagcacat caaggacaac aatgacgcga
    15121 aagcgatcgg cgtgaagatc acctatacga gcgaccagct cgccacctcg ctcaatacat
    15181 tcgacgggcc tgagctgcct catgacaaca cacactggcg tcacttcggc gatctgatcg
    15241 cttcgtataa ggccacagcg aagctgagtc tggccggctc gatcgatcgc ggacatcagg
    15301 agcttcccgg cgacgcagcc gccaactggc taggcattgc cggcttcgcc cgctacacgt
    15361 tcgacgaccg ccatgccgtg gccgtgcgcg gctctctctt ccacgatccg gacaatgccg
    15421 tcagcggcgc ggcgcagaag ctcaccgagg agacgctgac gtacgagttt cacccggtca
    15481 cgaacttgat cgtgaagatc gaagggcggc acgaccattc cactgcggcc gtcttcgatc
    15541 gcaagacaaa cgaatcgacc aacaaccaga ccatcgccgt catcggcgcg gtagtgactt
    15601 tctaggggac acatcatgtg ggatctgatt ttcgtcattc tgaccttcgc cctgtttgcc
    15661 ggatcgatct ggtacacgcg cgcttgcgac aaggtttgag gctgctatga cattcgaagc
    15721 catcgtcgga ctcgttctcg ctgtattcgc tttgatctac ctgctgtatg cgctggtccg
    15781 cgcggaagat ctttaatgac tacgctcgag ctattgaaaa tcgccatcta tttcctgttg
    15841 atcctaaccg tcacgaagcc gctcggactc tacatgcgtc gcgtgttctg cggcgagagg
    15901 accttcctcg atcctttact tcggccagtg gagcggttcg tttaccgcgc cggcggggtc
    15961 gacgagaagc gcgagcacga ctggatgcag tacacgatcg ccatgctgct cttcaatagc
    16021 gttggtttgc tgttcacgta cgccatcctg cgactgcagc atctccttcc gctcaatccg
    16081 gacaagctcg gtgctgttcc tcccgacctg gcctggaaca ccgccgtttc cttcaccacg
    16141 aatacgaact ggcaggcata cagcggcgag tcggcgatgt cgcatctctc acagatggtg
    16201 gcgctgacgt ttcac
//
LOCUS       DATLVW010000531         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_476368, whole genome shotgun sequence.
ACCESSION   DATLVW010000531 DATLVW010000000
VERSION     DATLVW010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476368"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..482)
                     /locus_tag="VNN25_15890"
     CDS             complement(60..482)
                     /locus_tag="VNN25_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93063.1"
                     /translation="MPPLEYITLAGLVVLAVLLWVYSRMRSKYRIDEILAKHRGSASV
                     CSRANLLEGMEMIPVALALKNDAIYYENTDIQASIELALIEEVEYDDETATGHTMPGK
                     VLRIRAHNHVFEFALDLPTARQWEAAFPPRRIDRARAV"
     gene            619..>2053
                     /locus_tag="VNN25_15895"
     CDS             619..>2053
                     /locus_tag="VNN25_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HXH93064.1"
                     /translation="MLRFSLAIAISAFLFVPSQAAAQSASASIQVAVTQPQPVVAGAN
                     FDYVINVSNEGPDDALTMTLTFPLPSGVLFQSAAAAAGWSCTHPSVGSNGTVSCTAPT
                     LAPGTVSFTITASTAPTAPAGTYSTTATITSTTPDPSDNDNSAQVDVIISTQSDFSIA
                     LAASPNPVDAGTNLTWTLTVTNNGPSTGTTASVSLPLPASTTFVSVAPPAGWSCITPP
                     VGANGTVTCSLTTSLSPSSSAVLTVVSQVDSSTASGSTLTGTATVSSPDDVFPVNDSA
                     TASVQTTASADLAVTKSVAPATDFPGSALQYTIIVSNNGPSNAATVTMTDVLPATLRF
                     TSLIAPGGWSCTTPAPGANGTVTCSLASMTVGSGASFTLNVIIASTTTPGTTINNTAQ
                     TGSSTPDANNANNSSTVQVVVAAPINVTASKPVNGAIHAEGSQVTYTIVLTNTGSVAQ
                     GDNPGNELTDVLPSSLSLISATSTSGTA"
BASE COUNT          406 a          686 c          585 g          376 t
ORIGIN      
        1 cgcgaaccac acacaacagc ggctgcggcg aaggcgattc ctccgccgtc gagatcgcgt
       61 cagactgcac gggcgcggtc gatgcgtctc gggggaaaag cggcctccca ctgccgtgcc
      121 gtcggcaggt cgagcgcgaa ttcgaagacg tgattgtgtg cgcggatgcg caggactttt
      181 cccggcatcg tgtggccggt agccgtctcg tcgtcgtatt cgacttcttc gatgagcgcc
      241 aactcgatcg acgcctgaat gtccgtgttc tcgtagtaga tggcgtcgtt cttcagggcc
      301 agagccacgg ggatcatctc catcccttcc aggagattgg cgcggctgca aacgcttgcc
      361 gatccgcgat gtttggccag gatttcgtcg atgcggtatt tgctgcgcat acgcgagtag
      421 acccagagca ggactgcgag taccacgagg ccggcgagag tgatgtattc gagcggtggc
      481 atcttgatcc tcctgtgacg actgtgtgac agaaaaaagc gtacgccttt tttgcgcgaa
      541 cgtcacgtcg ggacgctgga tttgatggcc tgacacataa tggccgcagc ggactcgtcc
      601 ccgtttgaga ggtaaataat gttgcgtttc tcccttgcaa ttgccattag tgcgttttta
      661 tttgtaccaa gccaggcagc agcccagtcc gcaagcgctt ccatccaggt tgccgtcacc
      721 cagccccaac ccgtggtcgc gggagccaac ttcgactacg tgatcaacgt cagcaacgaa
      781 ggcccggacg atgcgttgac catgacgctg acgttcccgc tgccgtccgg cgtcctcttc
      841 cagtccgcgg ccgcggcggc cggctggtcg tgtacgcatc cgtccgtggg cagcaacgga
      901 acggtgagct gcaccgctcc gacgctcgcg ccggggaccg tgtccttcac gattaccgct
      961 tcgaccgcgc caaccgctcc cgctggcacc tacagcacga cggcgacgat caccagcacc
     1021 acgccggacc cgagtgacaa cgacaactcc gcgcaggtcg acgtcatcat ctccacgcag
     1081 tccgatttca gcatcgcact cgcggcgtcg ccaaatccgg tggatgcggg aacgaacctg
     1141 acatggacat taaccgtcac gaacaacgga ccgagcacgg gaacgactgc ctcagtcagc
     1201 ctgccgctgc ctgcatcaac aacgttcgta tccgttgctc caccggcggg atggtcgtgt
     1261 atcacaccgc ccgtgggcgc caacggaacg gtcacctgct cgctcacgac atcgctgagc
     1321 ccctcgtcgt cggcagtatt aaccgttgtc tcgcaggtcg actcatcgac agcttcgggg
     1381 tcgacgctca caggaaccgc gaccgtctcg tcgccggacg atgtgttccc ggttaacgac
     1441 tccgcgacgg cgagtgtgca gacgaccgca tcggcggacc tcgcggtgac gaagagcgtg
     1501 gcaccggcga ctgactttcc gggaagcgcg ttgcagtaca cgatcatcgt ctccaacaac
     1561 ggaccgagca acgcggccac cgtcacgatg accgacgttc ttccggccac tcttcgcttc
     1621 acatcactga tcgcaccggg cgggtggtcc tgcacgacac cggcgcccgg tgccaatgga
     1681 acagtcactt gttcactcgc atcgatgacc gtcggaagcg gcgccagctt cacgctcaac
     1741 gtcatcatcg cttccacgac cacgccggga accacgatca acaacactgc gcaaactggc
     1801 agctccacac ctgacgcgaa caacgccaac aactcctcga ccgttcaagt cgtcgtggca
     1861 gcccccatca acgtcacggc ttcgaagccc gtcaacggag cgattcacgc ggaagggtcg
     1921 caggtaacct acacgatcgt cctcacgaac accggctcgg ttgcgcaggg cgacaaccca
     1981 ggcaacgagc tcaccgacgt attgccatcc tcgctctcac tcatcagtgc cacgtctacg
     2041 agcggaacgg ccg
//
LOCUS       DATLVW010000532         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_558859, whole genome shotgun sequence.
ACCESSION   DATLVW010000532 DATLVW010000000
VERSION     DATLVW010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558859"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VNN25_15900"
     CDS             <1..1337
                     /locus_tag="VNN25_15900"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HXH93065.1"
                     /translation="WWTAARKNTQIVISGSGAKANYSWSSSTGEADQTIKRDFDRAEP
                     KVKLDLARKHSARSKDLADYFSGKLAAEAARIAPRDPAAAWQIAATLETLPGEYTTLI
                     DPKTLITGPLASRVIAAMTDKPLRERALTQVREMHPEWPKVYTEVFFLDEEPRIMSLI
                     VAALEEGGHTDLRDRLIDETLRYPRRHPRAFYWFVKQLNEQETLTEKTNYNLLFQIID
                     AINSDEFSAVRARLKDMFDQGGLVLRIISGVDNEEQARKLVDTLDRYGAIEEYRREIA
                     KHAAMMKYPHLREPEAEPVYSTAQMLEQKRAELTNLKMVEIPANSRALQAAREMGDLR
                     ENFEYKAARARAEYLSARVGELAAEISRVQVLNPEQVDTSVVRVGTKVGLANGDERRE
                     VTILGPWESAPEHGVYSNQSDVAKALLGHATGDLVTFMGNDYQIESIRMWAS"
     gene            1576..2220
                     /locus_tag="VNN25_15905"
     CDS             1576..2220
                     /locus_tag="VNN25_15905"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXH93066.1"
                     /translation="MDRRPLKTRDRGWAKALAALLVRWRVRPNTISVLSVVFALGAGV
                     AFVASRDAEGVTRVVLLIAAAAGIQLRLICNMLDGMVAVEGKMASKTGDIFNELPDRI
                     ADPLIIVAAGYAIIRFYSLGPTLGWCAGLLAVMTAYVRVLAGSVRAKQDFVGPMAKPH
                     RMATLTFAGIVDAVAGYLRYRDYALMLALIVIIVGTIITMIRRTRRMAAELESR"
     gene            2217..2828
                     /locus_tag="VNN25_15910"
     CDS             2217..2828
                     /locus_tag="VNN25_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014822109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXH93067.1"
                     /translation="MRRVVAKGVVGIARVLAGGNVRRVGFTPDDRQRIYFVNHTSHLD
                     FVLVWAALPPRLRTMTRPVAAKDYWDKPGLRRYLGVKVFNAVLVERNPTTGSPNAIDV
                     LLDGLGEKHSLIIFPEGTRGDGTTIAQFKSGLYRLGKERPDVDLIPSYINNVNRVLPK
                     GEFVPVPMLASVSFGEPMHVGEDEDKNAFLDRARNAILELRQW"
     gene            2822..3760
                     /locus_tag="VNN25_15915"
     CDS             2822..3760
                     /locus_tag="VNN25_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXH93068.1"
                     /translation="MVTMIRDRQTLLLFAGILALLVVATLIGQIMRWRRRSDVVDNLN
                     ARIKAWWVMSAVFALSLLTGGLGSVILFGLLSFFALREFITLTPTRPADHSALFWVFF
                     IILPLQYYLVATQWYGFFSVLIPVYAFLFIPSRIAISGDANAFLERSSKIQWGLMVCV
                     YCVSHVPALLMLEIPGYAGKNARLLFFLVVIVQLSDVLQYVWGKLLGRHKISPVISPS
                     KTWEGFIGGTVSATLIGTALWRTTPFTVWQTMGMAFIITIMGFLGGLTMSAIKRDSGV
                     KDWGSSIQGHGGVLDRIDSICFAGPIFFHLTRYFFT"
     gene            3800..4066
                     /locus_tag="VNN25_15920"
     CDS             3800..4066
                     /locus_tag="VNN25_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXH93069.1"
                     /translation="MTYVIAEPCVNTKDTACVDVCPVDCIHPRKDEAGFAEDTMLYID
                     PEVCIDCGACEPACPVQAIFPEDLVPEQWKQYTQINADYYKKAT"
     gene            complement(4486..>4946)
                     /locus_tag="VNN25_15925"
     CDS             complement(4486..>4946)
                     /locus_tag="VNN25_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93070.1"
                     /translation="HDAVFCTPDEMFCAPDEMFCTPDEMFCTHDEMFCTHDEMFCAPD
                     EMFCTHDETFCAPDEMFCTHDEMFCTHDEMFCTDDAAACTHDTAVCTPDAAACTHDAV
                     VCSHDAAFCTRDAAVSTHDAAVCTDDAAFRTIDAVLSLNTAVKRPSHQEF"
BASE COUNT          937 a         1526 c         1525 g          958 t
ORIGIN      
        1 cctggtggac cgcggcgcgc aagaacacgc agatcgtcat cagcggcagc ggcgcgaagg
       61 caaactactc gtggagctcg tcgaccggcg aggccgatca gaccatcaag cgcgacttcg
      121 atcgcgcgga gccgaaggtc aaactcgatc ttgcgcgcaa gcacagcgcg cggagcaagg
      181 atctggcgga ctacttctcg ggcaagctcg ctgccgaggc cgcgcgcatt gcgccgcggg
      241 atccggcggc ggcgtggcag atcgctgcga cgcttgaaac gctccccggc gagtacacca
      301 cgctgatcga tccgaagacc ctcatcaccg ggccgctggc gtcacgggtc atcgccgcca
      361 tgaccgacaa gccgctccgc gagcgcgcgc tcacacaggt gcgcgagatg cacccggagt
      421 ggccgaaggt ctacaccgag gtcttcttcc tcgatgaaga gccgcgcatc atgtcgctca
      481 tcgttgccgc gctcgaagaa ggcggccaca cggacctgcg cgatcgtctg atcgacgaga
      541 cccttcgcta cccacgccgc catccgcgcg ctttctactg gttcgtcaag cagctcaacg
      601 aacaggagac gctgaccgag aagacgaatt acaacctgct cttccagatc atcgacgcga
      661 tcaactccga tgagttctca gccgttcgcg cgcggctcaa agacatgttc gatcagggcg
      721 gactcgtgct tcgcatcatc agcggcgtcg acaacgagga gcaggctcgc aagctggtcg
      781 acacgctcga ccggtacggc gcgatcgaag agtaccggcg cgagatcgcc aagcacgccg
      841 cgatgatgaa gtatccgcac cttcgcgagc ccgaggccga gccggtctac tccacggcgc
      901 agatgctgga gcagaaacgc gccgagctga cgaatctgaa gatggtggag atcccggcga
      961 actcacgcgc gcttcaggca gcgcgcgaaa tgggcgatct tcgcgaaaac ttcgagtaca
     1021 aagcggcgcg tgccagagcc gagtatcttt cggcgcgcgt cggcgaactg gccgcggaga
     1081 tctcccgcgt gcaggtgctc aatcccgagc aggtcgatac atcggtggtc cgcgtcggaa
     1141 cgaaagtcgg tctggcgaat ggcgacgagc gccgcgaagt aacgatcctc gggccttggg
     1201 agtccgcgcc ggagcacggc gtctactcca atcagtccga cgtcgccaaa gcgctcctcg
     1261 gccacgccac cggcgacctg gtgacgttca tggggaacga ttatcagatc gagtcgatcc
     1321 gcatgtgggc gagttagtgg ttgacgtagg tcggactctc cagtccgact tctcacgttt
     1381 ccgctacgaa cattcctttg tcggactgga gagtccgacc tacgtcggac tggagagtcc
     1441 gacctacgtt cgtccgcact tgagctatgt ctgactaacc gaatgacctg cgggccgcga
     1501 cgcagcaagc gtcgggtcac atatcactcc ttcacttcta ccgctcagtc cgtcgagact
     1561 acaatcgctt gattcatgga tcgtcgtcct ctcaagacgc gtgaccgcgg ctgggcgaaa
     1621 gcgctggcgg ctctgctggt gcgctggcgg gtgcgcccga acacgatctc ggtcctcagc
     1681 gtcgtctttg cgctcggagc gggcgtggcg ttcgtcgcga gcagggacgc cgagggtgta
     1741 actcgcgtcg tgttgttgat tgcggcggcg gcagggattc agcttcgtct gatctgcaac
     1801 atgctcgatg gcatggtggc cgtggaaggg aagatggcct cgaagacggg cgacatcttc
     1861 aacgaactgc ccgatcgcat cgccgatcca ctgatcatcg tcgcggccgg ctacgccatc
     1921 attcgtttct attcgctcgg gcccacgctc ggatggtgcg ccggtctgct cgcagtgatg
     1981 acagcgtacg tgcgagtcct ggccggctcc gtccgcgcga agcaggactt cgtcggcccg
     2041 atggcaaagc cgcatcgcat ggccacgctc acgttcgcag ggatcgtcga tgcggttgca
     2101 ggctacttgc gataccgcga ctatgcgttg atgctggcgc tgatcgtcat catcgtcggg
     2161 acgatcatca cgatgattcg gcggacgcgc cgcatggccg ccgagctgga gtcgcggtga
     2221 ggcgcgtcgt cgccaagggt gtcgtcggaa tcgcccgtgt tctggcggga ggaaacgtgc
     2281 ggcgcgtggg cttcacgccg gacgatcgac agaggatcta cttcgtcaac cacaccagcc
     2341 atctcgattt cgttctggtc tgggcagcgt tgccaccgcg tctacgcacc atgactcgtc
     2401 cggttgcggc gaaggattac tgggacaagc ccggcctgcg ccgctacctc ggcgtcaaag
     2461 tattcaacgc cgtcctggtc gaacggaacc cgaccaccgg atcgccgaac gcaatcgacg
     2521 tccttctcga cgggctcggc gagaagcact cgctcatcat ttttcccgaa gggacgcgcg
     2581 gagacggaac cacgatcgcg cagttcaaga gcggtttgta ccggctggga aaagagcgtc
     2641 cggacgtcga tctgattccg tcctacatca acaacgtcaa ccgcgttctg ccgaaagggg
     2701 agttcgtgcc ggtgccgatg ctggcctcgg tcagcttcgg tgagccgatg cacgtcggcg
     2761 aagacgaaga caagaacgcg tttctcgacc gcgcgcggaa tgcaatcctg gagctgcggc
     2821 aatggtgacc atgattcgcg accggcagac gctgctccta ttcgcaggaa tcctcgcgct
     2881 actcgttgtg gcgacgctca tcggccagat catgcggtgg cggcgccgca gcgacgtcgt
     2941 cgacaacctc aacgcgcgca tcaaagcgtg gtgggtgatg tcggccgtct tcgccctttc
     3001 gctgctcacc ggcggcctcg ggtcggtgat tctcttcggg ctcctctcct tcttcgcctt
     3061 gcgcgaattc atcacgctca cgccgacacg ccccgccgat cattccgcgc tcttctgggt
     3121 gttcttcatc atcctgcccc tgcagtacta cctcgtggcc acgcagtggt acggcttctt
     3181 cagcgtcctc atcccggtct acgccttcct cttcatccca tcgcgcatcg ccatctccgg
     3241 cgacgccaac gcgtttctgg aacggagctc caagatccag tgggggctca tggtctgcgt
     3301 ctattgcgtc agtcatgtcc cggcgctgct gatgctcgag attcccggct acgcgggcaa
     3361 gaacgcgcgg ctgctcttct ttctggtcgt gatcgtccag ctcagcgacg tgctgcagta
     3421 cgtctggggc aagctgctcg ggcggcacaa gatctcgccc gtgatcagtc cgagcaagac
     3481 gtgggaggga ttcatcggcg gaaccgtctc tgccacgttg atcggtacgg cgctgtggcg
     3541 tacgacaccg ttcacggtgt ggcagacgat gggaatggca ttcatcatca cgatcatggg
     3601 ctttctcggt ggactgacca tgtcggccat caagcgggac agcggcgtga aggactgggg
     3661 atcgtcgatc cagggacacg gcggcgttct cgaccgcatc gactccatct gtttcgcagg
     3721 ccctatattt tttcacttga cccgctactt cttcacttaa aatgccgccg cagttccagc
     3781 gtccaaggag cctatcccga tgacatacgt gattgccgaa ccgtgcgtga atacgaaaga
     3841 caccgcgtgt gtcgacgtct gcccggtcga ctgcatccat ccccgcaaag acgaggcagg
     3901 gtttgccgaa gatacgatgc tgtacatcga tcccgaggtc tgcatcgact gcggcgcgtg
     3961 cgagcccgcc tgcccggtgc aggccatctt cccggaggac ctcgtgccgg agcagtggaa
     4021 gcagtacacg cagatcaacg ccgactacta caagaaggcg acttgagttt tgtgagctgg
     4081 atcactatcc ttcagagatt gcgaacgtag actgacggcc acagcaacca gccgcaaggc
     4141 aaacaaaggg gcgtaatgcc ccttttttag tttctttgac attcgaacaa cctttggatc
     4201 gagatccttc agttacgctc ccacctcgga cccaaccacg gaagagctgg cgtatgggcg
     4261 tgcctccgtg gacattgctg aagctacctc tctctctctg caacgcgtgc cggagggtct
     4321 cgatcgaacg gctgttcgac ctcgcgggga tggtgagtga ggggtggctc gtggcagacg
     4381 tgcactcgtc atcacccgcg cgtttttccg gcgcgtacgc gtgagtcacc cgcagttcta
     4441 gttgacgccg gacacgacct ccactcaagt cttttgatac tcgagtcaaa actcttgatg
     4501 actgggtctt ttgacggcgg tgttcagcga caggacggca tcgatggttc gaaacgcagc
     4561 atcgtcggtg cagaccgcag catcgtgggt ggagacagca gcatcgcggg tgcagaacgc
     4621 tgcatcgtgg gaacagacca cagcatcatg ggtgcaggct gcagcatcgg gggtgcagac
     4681 cgcagtatcg tgggtgcagg ccgcagcatc gtcggtgcag aacatctcgt cgtgggtgca
     4741 gaacatctcg tcgtgggtgc agaacatctc atcgggggcg cagaacgtct cgtcgtgagt
     4801 gcagaacatc tcatcggggg cgcagaacat ctcatcgtgg gtgcagaaca tctcatcgtg
     4861 ggtgcagaac atctcgtcgg gggtgcagaa catctcatcg ggggcgcaga acatctcgtc
     4921 gggggtgcag aacacagcgt cgtgag
//
LOCUS       DATLVW010000533         9412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_149548, whole genome shotgun sequence.
ACCESSION   DATLVW010000533 DATLVW010000000
VERSION     DATLVW010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149548"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VNN25_15930"
     CDS             complement(<1..473)
                     /locus_tag="VNN25_15930"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HXH93071.1"
                     /translation="MTTSNPTDSSPSIVIPAVHRRAIIVDGHCDTPYRLIRHNVHIDE
                     HDPESQADLKSLRDSGITASFFAAYVPPFYAGRGAARFAYRLIELIHAEVKRRPDVLT
                     FCDDAAGIRRAKADGKTALMIGVEGGHAIEDSLEILRDFYRLGARYMTLTHVNTN"
     gene            752..1975
                     /locus_tag="VNN25_15935"
     CDS             752..1975
                     /locus_tag="VNN25_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93072.1"
                     /translation="MNLPLQIAWRYLRRPTDRLVSAVGFVSVLGLVIGVMALVISMAL
                     MTGYRGDLQKKLLGGNAEIFVYSVGGPIEDTKQAVATVRGTPGVAEAAPVLFQRALVT
                     TERNSTGTETMLKGIEPARAAGSPMIAKIVGPRGKFDTAAGERGVAVGRYLATKLGVA
                     AGQSISITVPAENGGSFMPRTASFVVTNVFDTGFYEFDARWLFIDLGEAEALLNAPGA
                     ANLLEVKLAPGADLDQVTRAVETRTEHRYAVSDWRDMNHQLFSMLKIQQLVLFIVIGL
                     IVFVSTFNIVSTLIMTVHEKRKEIGILSSMGAQSGTVTRIFVWYGTMVGFLGTATGIL
                     IGIVICWLITRFHLVSFPPEIAQVYFVSSIPFITRPVDLTIIAAFSIFVSFGATILPS
                     LRAARLNPVEALRHE"
     gene            2165..3271
                     /gene="carA"
                     /locus_tag="VNN25_15940"
     CDS             2165..3271
                     /gene="carA"
                     /locus_tag="VNN25_15940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXH93073.1"
                     /translation="MPTRLILEDGTVYDGDAFGATANTIGEVVFNTSLTGYQEIATDP
                     SYRFQIVVMTYPHIGNYGIEKAVAQNEVPQVAGFVVRDAIAEPSNAHSEMPLAEYFVQ
                     SGISAIQGIDTRSLTRKIRNEGAMRGMITSESGRDVASVVDEIRQAPSMSGLDLVQRV
                     TALRPYTFDEPPAEVKKRIALYDFGVKRDILRHLSRQGCDVTVYPATTHADEILAQRF
                     DGVMLSNGPGDPEPVTYAQENIRQLVGKIPIFGICLGHQLLGLALGGKTYKLKFGHRG
                     GNHPVKDLRSGRVEITAQNHGFAVDPDSLPDNDVERTHINLNDQTLEGFRHRREPVLA
                     VQYHPEAAPGPHDSFYLFGEFMQMIDEWKPERNR"
     gene            3357..3938
                     /locus_tag="VNN25_15945"
     CDS             3357..3938
                     /locus_tag="VNN25_15945"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HXH93074.1"
                     /translation="MTVNDPATSTYTPPPPAAPPAKKSGCMKWGLIGCGSLLVLGAIF
                     CVVIVVVVFGAMKKSDVYQEALKRAQSDPAVTAALGTPIEAGLMVSGSVNVDSGGGHA
                     TLDFPISGPKGKGSVHAVATKAGEKWEYTELTVTPANGAKINLLPPPTTTTTESSTTT
                     TTDTSATTTDTSATTDTTSTTDTATSTSSTSTQ"
     gene            complement(3971..4159)
                     /locus_tag="VNN25_15950"
     CDS             complement(3971..4159)
                     /locus_tag="VNN25_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93075.1"
                     /translation="MNDLLTVSSVLESTLADIDHQEEARRHENSDVTDVMNWIAGFRR
                     RSRVLAQNVQTLELSPVR"
     gene            complement(4287..5426)
                     /locus_tag="VNN25_15955"
     CDS             complement(4287..5426)
                     /locus_tag="VNN25_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH93076.1"
                     /translation="MSVSGNLKTMLPGDLLQWLSLGQKTGTLMVSNKGTQKKIFFSNG
                     RVISSASNDPREYLGQFLMSHGYITEEELMKAMEVQQQSGILLGKILVMIDVINEEDL
                     QRLMRLKAEEEIYDIFLWNEGDFQFIDDELPQMEMIPLRVDVTGLIMEGTRRVDEWNR
                     IHTLIAHEALVPTMVKEIPPEELEDDAQRTVINAIDGRRSLAELVLESRSSSFNVAST
                     VYHFTREGYVKLNDPTVMAAKAPEEIAPFEDSSDDDEILSLIARAQHSLRNREYEKAQ
                     RLLKAAENLDPSHMRVRNAIKGAESAILADLRGQGLHDSKVPRVSRALEEITQMNFTP
                     NEGFMLSRINGQWDLGSLIKISPIREPDAMLIFYKLWRDGIIALD"
     gene            5583..7142
                     /locus_tag="VNN25_15960"
     CDS             5583..7142
                     /locus_tag="VNN25_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXH93077.1"
                     /translation="MTDSKLFFDPDDLAARGFDPAHDLGEPGAFPYTRGPQASMYRGK
                     LWTMRQYAGFSTAAESNKRYRYLLERGTTGLSVAFDLPTQIGMDSDDPRARGEVGNVG
                     VAIDSIDDMETLFDAIPLDRVSISMTINAPASVLLALLLVVAERRGIAWKSLSGTIQN
                     DILKEYAARGTYIFPPAPSLRLVTDVFEFCGKEVPRWNTISVSGYHIREAGSTAVQEV
                     AFTLANGLQYVQAAVDRGLDIDSFAPRISFFFNAHNDFLEEVAKFRAARKLWAQLMRE
                     RYAPKDPKSLALRFHAQTAGSTLTAQQPENNVVRVAYQAMAAVLGGCQSLHTNSRDEA
                     LALPTEESAKIALRTQQVLAFESGVTQFADPLAGSYEIESRTLDIERGARALIERIDA
                     MGGARAAIESGFVQHEIGESAYLAQRALEEKKAIVVGVNEFVDDGSATLPIMSIDESV
                     ERDQVARLEAFRSSRKGDVRAALSVLDAAARNATNLMPAIVECVRQDCTVGEIVNTLK
                     ETFGEHKDQGF"
     gene            complement(7230..7682)
                     /gene="ybeY"
                     /locus_tag="VNN25_15965"
     CDS             complement(7230..7682)
                     /gene="ybeY"
                     /locus_tag="VNN25_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXH93078.1"
                     /translation="MTNVEVTGAAVARFSRREIAEFARRALIALDYLGRIASEISEVT
                     IAIVDDETMRNLNRKFRKKNKTTDVLTFPADDSDADPQAAGRPLGDIVISIDQARRQA
                     AEQRHSQATEVRYLILHGILHALGYDHETDDGEMNALEVEVRAAVGLE"
     gene            complement(7679..8641)
                     /locus_tag="VNN25_15970"
     CDS             complement(7679..8641)
                     /locus_tag="VNN25_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXH93079.1"
                     /translation="MELILDDEGTHTLFGNHDENLRMLEDEFSVKISSRGGEIFVTGA
                     SDNVAPVEKLLTQMQELIEQGYPLKRSDIHTSMRVLRDRPDASLVDFFTDDAVQASVR
                     RVVTPRNVAQKMYLQSIQDNDITFGIGPAGTGKTFLAVAAAAGALADKSVKRIVLCRP
                     AVEAGERLGFLPGDIAEKVNPYLRPLYDSLFDIVGFEKVEKLIERSVIEIAPLAFMRG
                     RTLNDAFIILDEAQNTTPEQMKMFLTRLGFGAKAVVTGDVTQVDLPTGAKSGLRVVRQ
                     ILEGIDRIRFFDFSDRDVVRHPVVQKIVMAYDRVDKEREKQEKV"
     gene            complement(8726..9046)
                     /locus_tag="VNN25_15975"
     CDS             complement(8726..9046)
                     /locus_tag="VNN25_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH93080.1"
                     /translation="MPIAEITPTDLKTLLSGSAPPLLIDVREEGEAAICSIDGSTLIP
                     MNTLPEHLAEIPIDRQVALYCHHGSRSWMVAQWMARNGYDALSLAGGIDRWALEIEPE
                     MNRY"
     gene            complement(9058..>9412)
                     /locus_tag="VNN25_15980"
     CDS             complement(9058..>9412)
                     /locus_tag="VNN25_15980"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HXH93081.1"
                     /translation="AIERDRKAVETIARLASEWSVPVTPIAADVFAGIPRLAAKVADG
                     AKFDVVYADPPYSFGRYDELLDTIGRHAPLADGALVGIEHQRRSDPFTIECKRLKAAR
                     RAEYGEIWITLFETA"
BASE COUNT         1734 a         2773 c         3043 g         1862 t
ORIGIN      
        1 ttgttcgtgt tgacgtgggt gagcgtcatg tagcgcgcgc cgaggcggta gaaatcgcgc
       61 aggatttcga gagagtcttc gatggcatga ccaccttcga cgccgatcat gagcgcggtc
      121 ttgccgtcgg ccttcgcccg acgaatgccc gcggcatcgt cgcaaaaggt cagtacatcc
      181 ggccgcctct tgacctcggc gtgaatgagc tcgatgagcc gataggcgaa ccgcgcggcg
      241 ccgcgcccgg cgtagaacgg cggcacgtag gcagcaaaga acgatgccgt gatgccgctg
      301 tcccgcagcg atttcagatc ggcctgcgac tcgggatcgt gctcgtcgat gtgaacgttg
      361 tgccggatca gacggtaggg cgtgtcgcaa tggccatcga cgatgatggc ccggcgatga
      421 acagccggga tgacgatgga cgggctggaa tcagtcggat ttgaagtagt catcagtagg
      481 gagtggggtt tggggcgtgg ggtgtgggag aagagccgtt cagattctaa tacgagagaa
      541 gttgatcgcg cagcacaagg gcggctcacg cgggtgtggg cggttgggtg tggggtgtgg
      601 gatgaatcct cgaagttcta ttaagagaga taatcctccg agagtctgat ccgagagacg
      661 attcttcgcg agatgggctc ctggctgtga ctccgggatt tactaccact ccccacaccc
      721 cactccccac accccgtaca ctaccgaccg aatgaacctc cctttgcaaa tcgcctggcg
      781 ctatctgcgg cgaccgacgg atcggttggt gtcggcggtg ggattcgtgt cggtgctcgg
      841 gctcgtgatc ggggtgatgg cgctggtgat ctcgatggcg ctcatgaccg ggtatcgggg
      901 cgatctgcag aagaagctgc tcggaggcaa cgcggagatc tttgtctact ccgtcggcgg
      961 cccgatcgag gataccaagc aggccgtggc gacggtgcgc ggcacgccgg gagtcgccga
     1021 ggcggcgccg gtgctgtttc aacgcgcgct cgtgacgacg gagcgcaact ccaccggcac
     1081 ggagacgatg ctgaagggga tcgagccggc gcgcgcggct ggctcgccga tgattgcgaa
     1141 gatcgttgga ccgcggggga agttcgacac ggcggcaggg gagcgtggcg ttgcggttgg
     1201 gcgttacctg gcgactaagc tcggcgttgc ggcgggccaa tcgatcagca tcactgtgcc
     1261 ggcggagaac ggcggcagct tcatgccgcg caccgcgtca ttcgttgtga cgaacgtctt
     1321 cgacaccggc ttctacgagt tcgatgcccg ctggctcttc atcgacctcg gcgaagcaga
     1381 agcgctgctc aacgctccgg gtgcggccaa cctgctggag gtgaagcttg cgccgggtgc
     1441 ggacctcgat caggtcacgc gcgccgtcga gacgcgcacc gagcatcgtt acgccgtctc
     1501 ggactggcgc gacatgaacc accagctttt ctcgatgctg aagatccagc aactggtgct
     1561 cttcatcgtc atcggcctga tcgtcttcgt ttcgacgttc aatatcgtct cgacgctgat
     1621 catgaccgtt cacgagaagc ggaaagagat cggcatcctc tcgtcgatgg gtgcgcagtc
     1681 ggggacggtg acgcgcatct tcgtctggta cggaacgatg gtcggcttcc tcggcacggc
     1741 taccgggatt ctcatcggca tcgtgatctg ctggttaatc acccgcttcc atctggtctc
     1801 gtttccgccc gagattgcgc aggtctactt cgtctcgtcg attccgttca tcacgcggcc
     1861 tgtcgatctg acgatcatcg ccgcgttctc gatcttcgtg tcgttcggag cgacgatcct
     1921 gccgtcgctg cgggcggcgc ggttgaatcc agttgaggca ttgcggcacg agtagcgagt
     1981 agtggatcaa tttgaaaaac cgatcaccac agaggcaaag aggtcacaga gatccctcgg
     2041 tgttctctgt gtctctgtgg ttgcttttgc ttcggcgatg acgataagcc gttcaaattg
     2101 acccacgacc gagtagcgag accgctcgac cgcatcgcaa ccccccaccg tataattcga
     2161 cgcaatgcct acccgcctga ttctcgaaga tggaactgtg tacgacggag atgccttcgg
     2221 cgcgactgcg aacacgatcg gcgaagtcgt tttcaacacc tcgctgaccg gctatcagga
     2281 gatcgccacc gatccttcct accggttcca gatcgtcgtc atgacctatc cccacatcgg
     2341 aaactacggt atcgaaaagg ccgttgccca gaacgaagtg ccgcaggttg ccggcttcgt
     2401 cgtgcgcgac gcgatagcgg agccgtcgaa tgcacactcg gagatgccgc tggctgagta
     2461 cttcgtgcag agcggcatct cggcgatcca gggaatcgac acgcgatcgc tcacgcgaaa
     2521 gattcgcaac gaaggggcga tgcgcggaat gatcaccagc gagagcggtc gtgacgtcgc
     2581 cagcgtcgtc gacgagatcc gtcaggcgcc ttcgatgagc ggactcgatc tcgtgcagcg
     2641 ggtgacggcg cttcgtcctt acacgttcga tgaaccgcca gccgaggtga agaagcggat
     2701 cgcgctgtat gactttggcg tgaagcgcga catcctgcgg catctttcga ggcagggctg
     2761 cgacgtgacg gtctatccgg ccacgaccca cgcggatgag atcctggcgc agcgcttcga
     2821 cggcgtgatg ctgtcgaacg gcccgggtga tcctgagccg gtcacgtatg cgcaggagaa
     2881 catccgtcaa ctcgtcggca agatcccgat cttcggtatc tgcctcggcc accagcttct
     2941 cggcctcgcg ctcggcggga agacctacaa gctgaagttc gggcaccgcg gcggcaacca
     3001 tccagtcaaa gacctgcgca gcggacgggt ggagatcacg gcacagaatc acggctttgc
     3061 ggtcgatcct gactcgctcc ccgacaacga cgtcgagcgc acgcacatca atctcaacga
     3121 tcagacactg gaagggttcc ggcatcgccg cgagcctgtg ctggccgtgc agtaccaccc
     3181 cgaggccgcg cccgggccgc acgactcgtt ctatctcttc ggggagttca tgcagatgat
     3241 cgacgagtgg aagccggagc ggaatcgctg atcgccgctc cgaagagagg caacctgtgt
     3301 agaatgcgcc ggtccccaca aatctacgag aatgaagtga agccggagga aacgccatga
     3361 ctgtgaacga tcctgccacc tcgacctaca cgccaccacc accggctgca ccgcccgcga
     3421 agaagagcgg atgcatgaag tggggactga tcggatgcgg ctccctgctc gtgctgggcg
     3481 cgatcttctg tgtcgtcatc gtcgtcgtcg tattcggcgc gatgaagaag agcgacgttt
     3541 accaggaagc gctgaagcgc gcacagagcg atcccgcggt tacggctgcg cttggaaccc
     3601 cgatcgaagc gggtctgatg gtgagcggca gcgtgaacgt tgacagcggc ggcggacacg
     3661 ccactctcga cttcccgatc tccggaccga agggcaaagg cagcgttcac gccgtggcca
     3721 cgaaggccgg ggagaagtgg gagtacacgg aactgacggt gacgccggcg aacggggcga
     3781 agatcaattt gctgccgcct ccgacaacga ctaccaccga gtcgtcgacc acgacaacta
     3841 cagatacctc ggcgacgacg acagatacgt cggcgacgac agacacgaca tcgaccaccg
     3901 atactgctac gagcacgagc tcaacgtcca cgcaataagc cggacgcgta ctttcgcgcg
     3961 tgaagagctc tcacctcacc ggtgagagct cgagcgtctg cacgttctgc gcgaggactc
     4021 gggatcgccg gcggaaaccg gcgatccagt tcatcacatc ggtgacgtcg ctgttctcgt
     4081 gccggcgtgc ctcttcctga tggtcgatgt ccgcgagtgt gctctcgagc acgctgctga
     4141 ctgtgaggag gtcgttcatt gccgggaagg cggacgcaaa ttacgtgcct gtgggactgt
     4201 cgtggtcggc aaacaagaca accacagaga cacagagaac acagaggggt ctctgtgacc
     4261 tctgtgtctc tgtggtgacc tggttttcaa tccagcgcga tgattccgtc ccgccagagt
     4321 ttgtagaaga tcagcatcgc gtccggctcc cggatcggtg agatcttgat cagcgagccg
     4381 aggtcccact ggccgttgat gcgggagagc atgaagccct cgttcggggt gaagttcatc
     4441 tgggtaattt cttccagcgc ccgggatacg cgcggcactt ttgagtcgtg cagcccctgg
     4501 ccgcgaaggt cggccaggat ggcggactcg gcccctttga tcgcgttgcg cacgcgcatg
     4561 tggctgggat cgaggttctc cgccgccttg agcagacgct gcgctttctc gtactcgcga
     4621 ttccgcagcg aatgctgggc tcgggcgatc agggagagga tctcgtcgtc atccgaggag
     4681 tcttcgaacg gcgcaatctc ctcaggagcc tttgccgcca tcaccgtcgg atcgttcagc
     4741 ttcacgtaac cctcgcgggt gaagtgatag accgtcgatg cgacattgaa tgatgacgaa
     4801 cggctctcca gcaccagctc agctagcgag cggcggccgt cgatggcgtt gatcaccgtt
     4861 cgctgggcat cgtcttcgag ctcctcgggc ggaatctctt tcaccatcgt cggcacgagc
     4921 gcctcgtgcg cgatcaacgt gtggatgcgg ttccattcgt cgacgcggcg cgttccttcc
     4981 atgatcaggc cggtgacgtc gacgcgcagc gggatcattt ccatctgcgg cagctcatcg
     5041 tcgatgaact ggaagtcgcc ttcgttccag agaaagatgt cgtagatctc ttcttcggcc
     5101 ttcaggcgca tgaggcgctg caggtcctct tcgttgatca cgtcgatcat gacgaggatt
     5161 ttgccgagga ggatgccgga ctgctgctgc acctccatcg ccttcatgag ctcctcctcg
     5221 gtgatgtaac cgtggctcat gaggaactgc ccaaggtatt cgcgcggatc gttcgatgcg
     5281 gaggagatca cgcgcccgtt gctgaagaag atcttcttct gcgtcccctt gttcgagacc
     5341 atgagcgtgc cggtcttctg cccgaggctc agccactgga gcagatcgcc cggaagcatc
     5401 gttttcaggt tgccgctgac actcatggtt gggcggtgcg caaagggggc tggattataa
     5461 gccgtacgcc aaagtgcctc tggtgttgcg aatcaccggt attcgcgctc atgcgacggt
     5521 agaacccgcc agagcacgtc gtgagccagc ggtaacgccc gggccactat gattgggtct
     5581 ccatgaccga ttcgaaactc tttttcgatc ccgacgatct cgccgcgcgt ggtttcgatc
     5641 ccgcccacga cctcggcgag cccggagcgt tcccgtacac gcgcggcccg caggcttcga
     5701 tgtatcgcgg caagctctgg acgatgcgcc agtacgcggg cttctccacc gcggcggaat
     5761 cgaacaagcg ttatcgctat ctgctcgagc gggggacgac cggtctctcc gttgccttcg
     5821 atctgccgac gcagatcggg atggactccg acgatccgcg tgcgcgcggc gaggtgggaa
     5881 atgtcggggt ggccatcgat tccatcgacg acatggagac gttgttcgac gccatcccgc
     5941 tcgaccgcgt ctcgatctcg atgacgatca atgcgccggc gtcggttctg ctggcgctgc
     6001 tgctcgttgt ggccgagcgc cgcggcatcg cctggaagag cctctccggc acgattcaga
     6061 acgacatcct caaggagtac gcggcgcgcg ggacgtacat cttcccgccg gcgccgtcac
     6121 ttcgtctcgt caccgacgtg ttcgagttct gcgggaagga agtgccgcgc tggaacacga
     6181 tctcggtctc cggctatcac atacgcgagg cgggatcgac ggccgtgcag gaggtggcgt
     6241 tcacgctcgc gaacggattg cagtacgtac aggcggccgt cgatcgcgga ctggacatcg
     6301 attcgttcgc gccgcgcatt tcgttcttct tcaacgcgca caacgatttc ctggaagagg
     6361 tggccaagtt ccgtgcggcg cgcaagctgt gggcgcaatt gatgcgcgag cgttacgcgc
     6421 cgaaggatcc aaagtcgctg gcgctgcgct tccatgctca gaccgcggga tcgacgctga
     6481 ccgcgcaaca gccggagaac aacgtggtgc gcgtggccta ccaggcgatg gccgcggttc
     6541 tcggcggctg ccagtcgttg cacacgaact cacgcgatga ggcgctggcg ctgccgaccg
     6601 aggagtcggc aaagatcgcg ctgcgcacgc agcaggtgct ggcattcgag agcggcgtga
     6661 cgcagttcgc cgatcctctg gctggatcgt acgagatcga atcgcgaacg ctcgacatcg
     6721 agcgcggcgc gcgcgccttg atcgagcgca tcgacgcaat gggcggggct cgcgcggcca
     6781 tcgaatcggg cttcgtgcag cacgagatcg gcgagtctgc ctacctcgcg caacgcgccc
     6841 tcgaagagaa gaaggccatc gtcgtgggcg tgaacgagtt cgtcgatgac ggatccgcga
     6901 cgctgccaat aatgtcgatt gatgaatcgg tcgagcgcga tcaggtggcg cggctggaag
     6961 cgttccgttc ttcccggaag ggggacgtgc gcgcggcgtt gtcagtgctc gatgcggctg
     7021 cacggaatgc cacgaatctc atgcccgcga ttgttgagtg tgttcggcag gactgcactg
     7081 ttggggagat cgtgaatacc ttgaaagaaa cgttcggcga gcacaaggat caagggttct
     7141 gacgcaagaa gcttttcacc acagagacac agagtgcaca gaggtttctc tgcgtcctct
     7201 gtgtctctgt ggtgaaaaag cttttgcagt cactccaacc ctacagccgc cctcacttcc
     7261 acttccaacg cgttcatctc gccatcgtca gtctcgtggt catagccgag cgcgtgcagg
     7321 attccgtgga ggatcaggta tcgcacctcg gtcgcctgcg agtgtcgctg ttcggcggcc
     7381 tggcggcggg cctggtcgat ggagatgacg atgtcgccga gggggcggcc ggctgcttgg
     7441 gggtcggcgt cggaatcgtc ggcggggaag gtgaggacat ccgtcgtctt gttcttcttg
     7501 cggaacttgc ggttgagatt gcgcatggtc tcgtcatcga cgatggcgat ggtgacttcg
     7561 ctgatctcag aggcgatgcg gccgaggtag tcgagcgcga tgagagctcg gcgggcgaac
     7621 tcggcgatct cgcggcggct gaaccgggcc acggcggcgc cggtgacttc gacgttcgtc
     7681 aaactttctc ctgcttttcg cgttctttgt cgacgcggtc gtaggccatg acgatcttct
     7741 gaaccaccgg atggcggacc acgtcacggt cggagaaatc gaagaaacgg atgcggtcga
     7801 tgccttcgag gatctggcgg accacacgca gcccggactt ggcgccggtg gggagatcga
     7861 cctgagtcac gtcgccggtg acgacagcct tcgcgccgaa gccgagccgc gtgaggaaca
     7921 tcttcatctg ctcgggcgtg gtgttctggg cctcgtcgag gatgatgaag gcgtcgttga
     7981 gcgtgcggcc gcgcatgaag gccagcggcg caatctcgat cacgctgcgc tcgatgagtt
     8041 tctcgacttt ctcgaagcct acgatgtcga agagcgagtc gtaaagcggg cgaagatagg
     8101 gattcacttt ctcagcgata tcaccgggta ggaagccgag gcgctcgccg gcttcgacgg
     8161 cggggcggca aaggacgatg cgcttgacgc tcttgtcagc cagcgcgcct gcggcggccg
     8221 cgacggcgag gaaggtcttg ccggtgccgg cgggaccgat gccgaaggta atgtcgttgt
     8281 cctggatcga ctgcaggtac atcttctggg cgacgttacg cggcgtgacg acgcggcgga
     8341 ccgaggcctg tacggcgtcg tccgtgaaga agtcgacgag gctggcatcg ggccggtcgc
     8401 gcagcacgcg catgctcgtg tggatgtcgg agcgcttgag cgggtatccc tgctcgatga
     8461 gctcctgcat ctgtgtcagc agcttctcca ccggtgcgac gttgtcggac gcgccggtga
     8521 cgaagatctc gccgccgcgc gaggagatct tcacgctgaa ctcgtcttcg agcatgcgca
     8581 ggttttcgtc gtgattccca aagagggtgt gtgtgccttc gtcgtcgagt atcagttcca
     8641 tttggccttt cgttgttatc cgcagatgac gcagatgaaa cgcagatgtt gcttctatct
     8701 gcgtttcatc tgcgtcatct gcggatcaat acctgttcat ctccggttcg atctcgagag
     8761 cccagcgatc gattccgcca gccagcgaca acgcgtcata gccgtttcgt gccatccact
     8821 gcgcgaccat ccaggagcgg gagccgtgat gacaataaag cgcgacctgg cgatcgatcg
     8881 gaatctccgc aagatgttcc ggcagcgtat tcatcggaat cagggtcgat ccgtcgatcg
     8941 agcagatcgc ggcttcccct tcctcgcgga cgtcgatcag gagcggcggt gcggagccgg
     9001 agagaagcgt tttcaagtcg gtgggcgtga tttcagcgat tggcatgagc atccttttta
     9061 agcagtctcg aacaatgtga tccagatctc gccgtactcc gcgcggcgag ccgctttcag
     9121 ccgtttgcac tcgatggtga atggatcgct gcgccgctgg tgctcgatgc cgacgagcgc
     9181 tccgtcggcg agcggcgcgt ggcggccgat cgtgtcgagc agctcatcgt agcggccgaa
     9241 cgagtatggc ggatcggcgt ataccacgtc gaacttagcg ccgtccgcaa ccttcgctgc
     9301 cagccgcggg attccagcga acacatcggc ggcaattgga gtcaccggaa cgctccactc
     9361 gctggcgagc cgggcgatgg tctcgacggc tttgcgatcc cgctcgatgg cg
//
LOCUS       DATLVW010000534         5574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_393392, whole genome shotgun sequence.
ACCESSION   DATLVW010000534 DATLVW010000000
VERSION     DATLVW010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393392"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..902)
                     /locus_tag="VNN25_15985"
     CDS             complement(120..902)
                     /locus_tag="VNN25_15985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH93082.1"
                     /translation="MSSAHATLHQLPTPVTVTVSSFDTTPIAYDLYDHASRSLVLVVP
                     GFWRDRRHPSMVRLASHLTELGYSTAILDPRGHGDSGGTYGFNLFEHYDTAVVAEDLL
                     HRLPIESITIVALSYGASVAISTAARHKLPIASLLLISAVADFAKIVPRFNPFTLHRH
                     IALSQALKRPRFDWHFRTSPKLRAIDDVRDVHAPICLIHVKNDWLIGHDHSLALYEAA
                     SEPKELHVIDIPGNYHADRIFSVAASEIDPVFGEFLRRYTPV"
     gene            1267..1602
                     /locus_tag="VNN25_15990"
     CDS             1267..1602
                     /locus_tag="VNN25_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93083.1"
                     /translation="MSKKIVVLLTVLALSVFGFACKSETTANNTDTAAAAADTSATTA
                     TTDTTVTATMATDTTGTTGSTSTSAMPSATDTSSTATTMTTATSTTGTTATTTTGTTK
                     TTTSSTKKH"
     gene            1866..2219
                     /locus_tag="VNN25_15995"
     CDS             1866..2219
                     /locus_tag="VNN25_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93084.1"
                     /translation="MKKTMILLTIVALAFFGYACKSETNNNNVTDTAAAATDTSTTAS
                     STSSTMSTTDTSMTGTTGTMSTDTTGTAAAMTGTDTTGTGAMSSTTGTTATSTTGTTG
                     TTTKTETKKTTTKKH"
     gene            complement(2269..2892)
                     /gene="coaE"
                     /locus_tag="VNN25_16000"
     CDS             complement(2269..2892)
                     /gene="coaE"
                     /locus_tag="VNN25_16000"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXH93085.1"
                     /translation="MILRAGLTGGIASGKSAVARMFAALGCVTIDADQIVTQLYQPGR
                     AGHQALVRTYGESILAPDGTIDRRKLADVALATDADAKKLNALIHPLVIEEQVRLIAA
                     EEAVVGDRIVIVEATLLIESGGRSRFDRIIVVDTDPETQIARAVARGMTREEAERRIA
                     RQMARDERLRFADYVIDSSGAMSDTEAATVRVYEALRKDLAAKKTPT"
     gene            complement(2889..3791)
                     /gene="folD"
                     /locus_tag="VNN25_16005"
     CDS             complement(2889..3791)
                     /gene="folD"
                     /locus_tag="VNN25_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXH93086.1"
                     /translation="MPAHLIDGKRVAKQVEEEVAAAIARLGIQPVLVVVRVGNDPASE
                     IYVRNKARKAEELGLRGDERIFPETLSEQDLLAEIDRLNRDETVDGILVQLPLPKHID
                     PHRVIDAIDPAKDVDGFHPQNVGRLHLGRPTLTPCTPAGVIRLLDAYDLQIEGKHAVV
                     IGRSDIVGKPVAALLLQRNATVTICHSRTKDLTAIARKADIIVAAIGKPFFVTAEMVK
                     GGAAVIDVGINRIDASHASLVAHDEIKSGLLAKNGHAVVGDVDFVRVREVAAWITPVP
                     GGVGPMTIAMLMSNTVKAAEWRRR"
     gene            4080..4511
                     /gene="rplM"
                     /locus_tag="VNN25_16010"
     CDS             4080..4511
                     /gene="rplM"
                     /locus_tag="VNN25_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXH93087.1"
                     /translation="MRTYFPKQGDIEPKWFIIDAEGKILGRLSTEIARIISGKSKPTY
                     TPFLDTGDHVIIINAEKIVLSGKKETDKIYRHHSLYPGGLSERAARFVRAEKPESMIE
                     RAVLGMLPHNKLGRKMIKKLKVYRGTNHPHEAQKPERLEVA"
     gene            4529..4921
                     /gene="rpsI"
                     /locus_tag="VNN25_16015"
     CDS             4529..4921
                     /gene="rpsI"
                     /locus_tag="VNN25_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXH93088.1"
                     /translation="MALVEYYGTGRRKTSTARVHLRQGSGAITVNHRSLDEYFGNDVL
                     KMIIKQPLSLTETGDKFDIVVTVDGGGPSGQAGAIRHGISRALQIFNVELRKRLKKAG
                     LLTRDPRMKERKKYGQKGARARFQFSKR"
     gene            5054..>5574
                     /gene="rpsB"
                     /locus_tag="VNN25_16020"
     CDS             5054..>5574
                     /gene="rpsB"
                     /locus_tag="VNN25_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXH93089.1"
                     /translation="MKELLEAGVHFGHQTKRWNPKMKKYIFGKRNGIYIIDLQKTLKL
                     FKEASAFISDLASQGKRILFVGTKRQAQDAILEEANRCGMFYVNNRWLGGTLTNFTTV
                     RKSIERLKEIESIIGDTDKEMSKKERAALDREREKLLKNLIGIREMDGLPDALFVIDP
                     KKEYIAIQEAKKLG"
BASE COUNT         1174 a         1709 c         1665 g         1026 t
ORIGIN      
        1 tccgtgggga tcgggaggcc ggttacgcct tggtgcgccg gcttgatcgg ttgggaatcg
       61 tgaggtcgcg agtcggcagt cgtaacaaac gcaaacccac ttccccgact ccccacaccc
      121 tacactggcg tgtaccgccg caagaactcc ccgaacaccg gatcgatctc actggcggcc
      181 acggagaaga tgcgatcggc gtggtagttg ccggggatgt cgatgacgtg caactccttt
      241 ggctcgctgg cggcttcgta gagcgcgagc gagtggtcgt ggccgatcag ccagtcgttc
      301 ttgacgtgga tcaggcagat cggggcgtgg acatcgcgaa cgtcatcgat cgcccgaagt
      361 ttggggctgg tgcgaaagtg ccaatcgaac cgcggccgtt tcagcgcttg agaaagggca
      421 atgtgcctgt gcagcgtgaa gggattgaac cgcggcacga tcttcgcgaa atcggcgacg
      481 gctgaaatca gcaagaggga tgcgatcggc agcttgtgcc gcgccgccgt cgagatggcc
      541 accgacgctc cgtacgagag agccacgatc gtaatcgact cgatcggaag ccggtgaagg
      601 agatcctccg ccaccacggc ggtatcgtaa tgctcgaaga ggttgaagcc gtaggtgccg
      661 ccgctgtcgc cgtgtccccg aggatcgagg atggcggtgc tgtagccgag ctcggtcagg
      721 tgagaggcca ggcgcaccat cgacggatgg cgccggtcgc gccagaaccc agggacgacg
      781 aggaccagcg agcgcgaggc gtgatcgtaa agatcgtagg caatcggggt ggtatcgaac
      841 gacgaaacgg tgacggttac cggggtgggg agttggtgca gcgtggcgtg tgcgcttgac
      901 atggtggtgt gcttcctaga ctatagccgc tcgccgggcg cttttcgctc gcgtcccccc
      961 tgcagtcttg ccctgatgcg gtacgccccc ggagatcgta cggaagtaga agttctttgt
     1021 tgccgcgatg cctgcggtgt gatcctgcaa gcccgtgttg ccattttcga gggactctcg
     1081 aaagagctgg catcgtcgtt gcgaatcagg tagaactcgt tttttataca cgttgcgaag
     1141 cggcccgctt cgccagaccc atcaattcga ttgccaaggg gggtgagcag gcacttttca
     1201 tcggatccac cccaggtcaa aagacatcaa caacgcaaca aaaataacga gaggaaaaaa
     1261 agacaaatga gcaagaaaat tgtagttctc ctgacggttc tcgccctgag cgttttcggt
     1321 ttcgcctgca agagcgagac gaccgccaac aacaccgaca ccgcggcagc tgcggctgac
     1381 acgtctgcca ccaccgcgac caccgacacc acggtgacgg ccacgatggc taccgacacg
     1441 accggcacga ccggctcgac ctcaacctcg gcgatgccga gcgccacgga tacgtccagc
     1501 acggccacga cgatgacgac ggctacgtca acaacgggca cgactgctac gaccaccacg
     1561 ggcacaacga agacgacgac ctcgagcacc aagaagcact aacaacgtca tctccctgtt
     1621 tcaagacgcc ccgcgcaacc cgcggggcgt ccttaatagc tgttagcact ggtgccgtaa
     1681 ccggattcct ttttcctccc gttccgatct cgattacgat ccgcgagaca aaatctcgct
     1741 gcaattcatg atttgacatc gaagcaatgc gaaggaggtg aggcaggcac tcccagaaaa
     1801 cgacatcagc attttccatg tcgcagcaac agaacatcaa cttcgtctga gaggaaaaaa
     1861 cacacatgaa gaagacaatg attctcctga ccatcgtcgc actcgcgttc ttcggctacg
     1921 cctgcaagag cgagacgaac aacaacaacg ttaccgacac cgccgcagcc gcgacggata
     1981 cctcgaccac ggcgagcagc acgagctcga ccatgtcgac caccgacacc tcgatgaccg
     2041 gcacgaccgg cacgatgtcg accgacacga cgggcaccgc cgcagcgatg acgggcacgg
     2101 acacgacggg cacgggcgcg atgtcctcca ccaccggaac gaccgcgacc agcacgaccg
     2161 gtacgacggg caccaccacg aagaccgaga cgaagaagac caccacgaag aagcactaac
     2221 cttcgctcaa ttgctgttca acgccccgcc acccggcggg gcgttttttt atgtgggagt
     2281 cttcttcgcg gcaaggtctt tccgcaacgc ctcatacacg cggaccgtcg cggcctcggt
     2341 gtcactcatc gcgccgctgc tgtcgatgac gtaatcggcg aaacgaagcc gctcatcgcg
     2401 cgccatctgc ctggcgatgc gccgctctgc ttcctcccgc gtcatccctc gagccacggc
     2461 gcgcgcgatc tgggtctcag ggtcggtatc gacgacgatg atgcggtcga agcggctcct
     2521 cccacccgac tcgatgagca gcgtcgcctc gacgatgacg atgcggtcgc cgacgacagc
     2581 ttcttctgcg gcaatgaggc gcacctgttc ctcgatcacg agcggatgaa tgagcgcgtt
     2641 gagctttttc gcgtcggcat cggtggcgag agcgacatcg gccagcttgc ggcgatcgat
     2701 cgtaccgtcc ggtgcaagga tcgactcacc gtaggtgcga acgagcgcct gatgaccagc
     2761 gcgccccggc tgatagagct gcgtcacgat ctgatccgcg tcgatcgtca cgcatccgag
     2821 tgcggcgaac atgcgggcga cggccgactt tccgctggcg attccgcccg tcagtccggc
     2881 gcgaaggatc atcgccgccg ccactccgcc gccttgaccg tgttgctcat gagcatggcg
     2941 atcgtcatcg gaccgacgcc gcccggcacc ggcgtgatcc acgccgccac ttcgcggacg
     3001 cgcacaaaat cgacatcgcc gacgacagcg tggccgttct tcgcgagcag gcccgacttg
     3061 atctcgtcgt gcgccacgag cgatgcatgg ctggcgtcga tgcggttgat gcctacatcg
     3121 atcacggctg ctccaccttt caccatctct gcggtcacaa agaacggttt gccgatcgcg
     3181 gcgacgatga tgtcggcctt gcgcgcgatg gcggtgaggt ccttcgtgcg cgagtgacag
     3241 atcgtgacgg tcgcgttccg ctgaaggaga agcgccgcta ccggtttgcc gacgatgtcg
     3301 ctgcgaccaa tcaccacggc gtgcttccct tcgatttgta gatcgtatgc gtccagcagg
     3361 cgaatgacgc ccgcgggcgt gcacggcgtc agcgtcggcc ggccaaggtg cagacggccg
     3421 acgttctggg gatgaaagcc atccacgtct ttggcgggat cgatcgcatc gatgacgcgg
     3481 tgaggatcga tgtgtttggg cagaggaagc tgcacgagaa tgccgtcgac ggtctcgtcc
     3541 cgattcagcc ggtcgatctc cgcgagcaga tcctgctcgg aaagcgtttc gggaaagatg
     3601 cgctcgtcgc cgcgcagtcc gagctcctcc gccttgcgcg ccttgttccg aacatagatt
     3661 tcggacgcgg ggtcgttgcc gacacgaacg accacgagca ccggttggat gccgagtctg
     3721 gcgatcgcgg ctgcgacctc ctcctccacc tgtttggcca cccgtttccc gtcgatgagg
     3781 tgtgctggca ttcgtgctgt tttgtatcac gttgccgcat ccggattggc cgcgaggagc
     3841 gacaggaatt ctccgcgccg gtcgcgcctt accacgcgag cgcccggtaa ccccttcctg
     3901 caccagcaga ttgggaatga atttcttgac agtggctgtt cgggcggtgt accgttttgc
     3961 ccctttcccg tggggaaggt gtgtggttgt ctttgaatga acgtggcgct gagcagttcc
     4021 cctgtgccgc gatcctccga agatcgaatc tgagttgcga agtgtattca gggagagtca
     4081 tgcgtacgta tttccccaag cagggcgaca tcgagccgaa gtggttcatc atcgacgccg
     4141 aaggcaaaat cctcgggcgc ctttcgaccg agatcgcccg gatcatttcc ggcaaaagca
     4201 agccgaccta cacgccgttc ctcgacaccg gtgatcacgt gatcatcatc aacgccgaga
     4261 agatcgtgct atcgggcaag aaagaaaccg acaagatcta ccgtcatcac tcgctctatc
     4321 cgggcggact cagcgagcgt gccgcgcgct tcgttcgtgc ggaaaagccg gagtcgatga
     4381 tcgagcgcgc cgttctgggc atgctgcccc acaacaagct cggccggaag atgatcaaga
     4441 agctgaaagt ctatcgcggg accaatcacc cgcatgaagc gcagaaaccc gagcggctcg
     4501 aagtcgcttg acaggtcagg aggattcttt ggcactggtt gaatactacg gaacgggacg
     4561 ccgcaagacg tcaaccgctc gcgtccacct ccgtcagggt agcggtgcga ttacggtcaa
     4621 tcaccgctca ctcgacgagt acttcggcaa cgacgtgctg aagatgatca tcaagcagcc
     4681 tctttcgctc acggagaccg gcgacaagtt cgacatcgtc gtcaccgtcg acggcggcgg
     4741 gccgagcggt caggcgggtg caatccgtca tggcatctcc cgcgcgctgc agatcttcaa
     4801 tgtcgagctc cgcaagcgcc tgaagaaggc cggcctgctc acgcgcgatc cgagaatgaa
     4861 agagcgcaag aagtacggtc agaagggcgc ccgcgcccgc ttccagttct ccaagcgcta
     4921 gttccgatgt tttcgtttcc cccagcttga tttccgaaga caggagcgca gctcgctcct
     4981 gtcttttttt tgactcacgc cgttcaacca acgtcactcg agaaggaggg tttgcttggc
     5041 agtctctatt acgatgaaag agcttttgga agctggcgtc cacttcggtc atcagaccaa
     5101 gcgctggaat ccgaagatga agaagtacat cttcggaaag cgcaatggga tctacatcat
     5161 tgaccttcag aagacgctca agctctttaa ggaagcttcc gcgttcatct cggacctcgc
     5221 gtcccagggg aaacgcattc tatttgtcgg caccaagcgc caggcgcagg atgccatcct
     5281 cgaagaggcc aaccgctgcg gcatgttcta cgtgaacaac cgctggctgg gcggcacact
     5341 gacgaacttc accaccgtcc ggaagtcgat cgagcgcctc aaggaaatcg agtcgatcat
     5401 cggcgacacc gataaagaga tgtcgaagaa ggagcgcgca gccctcgacc gcgagcgcga
     5461 gaagctcctc aagaatctca tcggtatccg tgagatggac ggccttccgg atgcgctctt
     5521 cgtgatcgat ccgaagaagg agtacatcgc catccaggaa gcgaagaagc tcgg
//
LOCUS       DATLVW010000535         3524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_232879, whole genome shotgun sequence.
ACCESSION   DATLVW010000535 DATLVW010000000
VERSION     DATLVW010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232879"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VNN25_16025"
     CDS             complement(<1..1569)
                     /locus_tag="VNN25_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXH93090.1"
                     /translation="MRRWVCPLVIAVSVSLIGAFPVPGQELPFRHLTPNDQVNPLSSA
                     SVQKITQDHLGYIWFAFYASGLTRYDGHAMEQYGTADGLRDLTIREVAEDGTHHLWAG
                     SESGLVVSEKPLDVYEPGKRVRFVSAVGNERLPQMRVRRNCLVTGIDGWVWAGVQDRI
                     VRYRFHGDALERGLIDEVPTTSGVSAMLMRRDGSVLISLNSGVVLQFDREGKFGGELH
                     GLPSCVNGVLIEARDGALWGGCSNGVLWRGQGEHYEIINHTLTERIVSILETSRGELW
                     VASLGSGAMRLDTRNFADVLLVKRQNDLLGDTLWDLFEDREGNLWFAQNGGASRLRKD
                     YRAFEALTGQSHAGEQPALPDPGAFSVLAPDSGARGPWANLTIVGTGGGLALIAPDKT
                     TGEGVAQVAPDKTQSVLRVGDGLLSNSVYSLSRDAEGRIWIGTVGGVNCLSTPANAPP
                     MMTSSRRNNATVAGTSAVITSYPVDTTYLVQPFTIRGAETVWLAGIHGIVCLAGGQWF
                     TFGTAAGLPPAGASA"
     gene            1625..2731
                     /locus_tag="VNN25_16030"
     CDS             1625..2731
                     /locus_tag="VNN25_16030"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXH93091.1"
                     /translation="MAELQDWVELGKDTISSLRTKDVTNLYVLEWPETRKMLIADYQS
                     KIDADPKRLRRFVRTRSAVLLGLAKRLTPQRRLVFLVAFVWFFACLLGVLSWRNHIQN
                     PWLLFEMTGAFMLVILLLAMELIDKIKFRDELELARDLQADLIPKHPPQTDEYEIAAF
                     NRIANTVGGDIYDFVPLPDGRLAVLFGDASGHGMAAGLVMAVAHAAWRTQLDVDPSPA
                     AIISSLNRILCRTGGPRSFFSCCYFLFDRDGTFIATVAGHPQIVKIAAGKVVDRIGSG
                     SYPLGVKTGMVWQEVHGSLDRGERLLFHSDGLTEARNANEREFGDSFVDAIAGWNPTA
                     SAATLVETLVGEWNLFTGGRPPEDDVSIAVIARR"
     gene            2769..>3524
                     /locus_tag="VNN25_16035"
     CDS             2769..>3524
                     /locus_tag="VNN25_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93092.1"
                     /translation="MKASSAIAMLVCVAFIAVAPRFASQNAIAVLWVELAALLALMIT
                     VGVAVTGRFWGILINERNVMSLSRFQAVLWTVLVLADFATILLGRAWKGMSLSNAQDL
                     LRPDLLMLMGISLGSAVGTSIVNANKSGKPTPATAVQDAQANMHDQAGDFTAAQGVMY
                     KNKSITDASLGDLFQGDELADAHMTDLSKVQMFFFTIVSAVVFLGTADAMFTNMTLGA
                     VTVPQLPQTLIALMGISHATYLGNKAVTRTQTQP"
BASE COUNT          663 a         1175 c         1054 g          632 t
ORIGIN      
        1 cgcactggca ccggcaggcg gaagcccggc cgccgttccg aacgtaaacc actgcccgcc
       61 agcgaggcag acgattccgt gaatccccgc cagccagacc gtctccgcgc cgcgaatcgt
      121 gaaaggctgc acgaggtagg tcgtgtcgac cggataggaa gtaatcacgg cggatgtgcc
      181 ggcaacggtg gcgttgtttc gcctcgacga cgtcatcatc ggcggtgcgt tggccggcgt
      241 cgacaagcag ttcaccccac ctaccgtccc aatccagatc cgcccctcgg catcgcgcga
      301 cagcgaatag accgagttgc tgaggagacc gtccccgacg cgcaacacac tctgcgtctt
      361 gtcgggcgcg acctgcgcca cgccttcgcc cgtcgtcttg tcgggggcaa tgagcgccag
      421 gccgccaccg gtgccgacga tcgtcaggtt tgcccacggc cctcgggctc cggaatcagg
      481 agccagcacc gaaaacgcgc ccggatcggg gagcgctggt tgttcgcccg catgcgactg
      541 gcctgtcagc gcttcgaacg cgcgatagtc cttccgtagc cgggacgcac cgccattctg
      601 agcgaaccag aggttgcctt cgcgatcctc gaagagatcc cacagcgtgt cgccgaggag
      661 atcgttctgc cttttcacga gaaggacatc ggcgaaattg cgagtgtcga gacgcatcgc
      721 tccggagccg agactggcca cccagagctc gccgcgcgat gtctccagga tcgacacgat
      781 tcgctccgta agcgtgtgat tgatgatctc gtagtgctcg ccctgcccac gccagagaac
      841 accattcgag catccgcccc atagcgcgcc gtcgcgtgcc tcgatcagca cgccattcac
      901 gcacgatggc aagccatgaa gctccccgcc gaacttaccc tcgcggtcga actggagaac
      961 gacgccggag ttgagtgata tcagtaccga gccgtcccgg cgcatgagca tggccgagac
     1021 tccggaagtc gtcgggacct cgtcgatcag gccgcgttcg agggcgtcgc cgtgaaaacg
     1081 gtagcggacg atccggtcct gcacgcccgc ccagacccac ccgtcgatac cggtgacgag
     1141 acagttccgt cggacgcgca tctgcggcag ccgttcgttg ccgaccgcgc tgacgaagcg
     1201 cacgcgcttt ccaggctcgt agacatcgag cggcttctcc gaaacgacga gtcccgattc
     1261 ggatcccgcc caaaggtggt gggttccgtc ttcggccacc tcgcggatgg tcaggtcacg
     1321 caatccatca gccgttccgt attgctccat ggcatggccg tcgtagcgcg tcagaccgga
     1381 agcgtagaag gcgaaccaga tgtagccgag gtggtcctgc gtgatcttct gaacgctggc
     1441 cgaggagagc ggattgacct gatcgttcgg cgtaaggtgc cggaacggaa gttcctgtcc
     1501 cggaacagga aacgcaccaa taagcgaaac gctgaccgct atgacgagtg ggcacaccca
     1561 tcgacgcatc ggcgcagtct accgctccgg aaccccctcg cgacttctta cgtacaatcc
     1621 agccgtggca gagcttcagg actgggtcga gctcggtaaa gacacgattt cgtccttaag
     1681 aacgaaagat gtgacgaatc tctacgtgct cgagtggccc gagacgcgga agatgctgat
     1741 cgccgactac cagtcgaaga tcgacgccga cccgaagcgt ttgcgccgct tcgtgcgcac
     1801 ccgcagcgcg gtgctcctcg gcctggcgaa gcgtctcacg ccccagcgcc gcctggtgtt
     1861 tctggtcgcg tttgtctggt tcttcgcctg cctcctcggc gtcctgagct ggcgcaatca
     1921 cattcaaaac ccctggctgc tcttcgagat gaccggagcg ttcatgctcg tgatcctgct
     1981 gctggcaatg gagctgatcg acaagatcaa gttccgcgac gaactcgagc tcgcgcgcga
     2041 tctgcaggcg gatctcatcc cgaaacaccc tccgcagacg gacgagtacg agatcgcggc
     2101 tttcaaccgg atcgcgaaca cggtcggcgg cgacatctac gacttcgtgc ctcttcccga
     2161 cggacgcctc gccgtccttt tcggggatgc ctcggggcac ggaatggccg caggtctggt
     2221 catggctgtg gcgcacgcgg cgtggcgcac gcaactcgat gtcgatccgt cgccggcggc
     2281 catcatcagt tcgctgaacc gcatcctctg ccgcaccggc ggaccgcgtt ccttcttctc
     2341 atgctgctac ttcctgttcg accgcgacgg aacgttcatc gccaccgttg ccggccaccc
     2401 gcagatcgtg aagatcgcgg ccggaaaagt agtcgaccgc atcggcagcg ggtcctatcc
     2461 cctcggcgtg aaaaccggaa tggtctggca ggaagtgcac ggctcgctcg atcgaggcga
     2521 acggctgctg ttccattccg atggcctgac cgaggcgcgg aatgcgaacg agcgcgagtt
     2581 cggcgactcc ttcgtcgacg ccatcgccgg ctggaaccct accgcctcgg cggccacact
     2641 cgtcgagacc ctcgtcggcg aatggaatct cttcaccggc ggacgcccgc ctgaagatga
     2701 cgtctcgatt gccgtaatcg cccgccggta gtagcctttt ggcaccccaa acaaacagga
     2761 ggtccatcat gaaggcatct tccgcaattg ccatgctcgt ctgcgtcgca ttcatcgcag
     2821 tagcgccgcg gttcgcgtcg caaaacgcca tcgcagtgtt gtgggtcgaa ctggccgcgc
     2881 tgctcgcgct gatgatcacg gtcggcgtcg ccgtgacggg ccgcttctgg ggaatcctca
     2941 tcaacgagcg caacgtaatg agcctctcgc ggttccaggc cgtgctctgg accgtgctcg
     3001 tgctcgccga tttcgccacg atccttctcg gccgcgcgtg gaaagggatg tcgctctcga
     3061 acgcgcagga tctgctccgg cccgatctgc tgatgttgat gggaatcagc ctcggatcgg
     3121 cggtcggtac atcgatcgtc aatgcgaaca agtcaggcaa gccgacaccg gcgacagcag
     3181 ttcaggacgc gcaggcgaac atgcacgatc aggccggcga cttcaccgcc gcgcagggcg
     3241 tcatgtacaa aaacaagagc atcaccgacg cctcgctcgg cgatctgttt caaggcgatg
     3301 agctggccga cgcccacatg acggatcttt ccaaagtgca gatgttcttc ttcacgatcg
     3361 tatccgccgt cgtgttcctc ggcaccgcgg acgcgatgtt caccaacatg accctcggcg
     3421 ccgtcaccgt gccgcagcta ccgcagacgc tcatcgcgct gatgggcatc agtcacgcga
     3481 cgtatctcgg caacaaagca gtcacgcgca cccagacgca gccg
//
LOCUS       DATLVW010000536         8282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_434121, whole genome shotgun sequence.
ACCESSION   DATLVW010000536 DATLVW010000000
VERSION     DATLVW010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434121"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="VNN25_16040"
     CDS             complement(<1..1854)
                     /locus_tag="VNN25_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXH93093.1"
                     /translation="MTTGIGIDKATELSPRDFERMLLRTLTSMKKGDFKVRMPVEFTG
                     AAGKIADTLNDILDLNQRTAEEVEKISTVVGKEGKLNQRAQVPNAAGQWAVITESVNS
                     LIGDLVQPTNEISRVIGAVARGDLSRTMAIEFDGRPLKGEFLRTAKTVNTMVDQLASF
                     ASEVTRVAREVGTEGKLGGQADVKGVAGTWKDLTDSVNSMASNLTGQVRNIAEVTTAV
                     ARGDLSKKISAEARGEILELKNTINTMVDQLRSFASEVTRVAREVGSEGKLGGQADVQ
                     GVSGTWRDLTDSVNYMAANLTNQVRNIAAVTTAVANGDLSKQITVDARGEILELKETI
                     NTMVDQLRSFASEVTRVAREVGTEGKLGGQAEVKGVAGTWKDLTDSVNSMASNLTGQV
                     RNIAEVTTAVANGDLSKKITAEALGEVLELKETINTMVDQLRSFASEVTRVAREVGTE
                     GRLGGQAEVPGVAGTWKDLTDSVNAMASNLTGQVRNIADVTTAVANGDLSKKITAEAL
                     GEVLELKNTINTMVDQLRSFASEVTRVAREVGTEGELGGQADVQGVAGTWKDLTDSVN
                     SMAGNLTSQVRNIAEVTTAVASGDLSKKITVDVRGEILELKDTINTMVDRLN"
     gene            complement(2216..2680)
                     /locus_tag="VNN25_16045"
     CDS             complement(2216..2680)
                     /locus_tag="VNN25_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93094.1"
                     /translation="MPYVALEWDHKIGRPVIQWLVVKCQSVGEKFGGPQALPPRRDVA
                     YFVDEQTAERDAKAFAAYKTRIDDGGTDSDQKLGKSEAYSKKHAAFGWDHNIFNPLVQ
                     WAVLEWNGENPEGESFRNDVAYFLDPETGEADARAFRVLRDELVAKAAKIAA"
     gene            2875..5106
                     /locus_tag="VNN25_16050"
     CDS             2875..5106
                     /locus_tag="VNN25_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH93095.1"
                     /translation="MVLSLDSQSQRRSRLLDAMPAIAWSADAQTFRFTYVNPAAETLL
                     GYPVERWLDEPGFWIEHLHPDDRHVAMICHNETLAARDHELVYRIIAADGRTVWLRDY
                     VNVHCEDGMPVELFGVMVDITREREAEAASRENRENFRRMVELSPDCIGVHVDETYVY
                     VNQAFVHLLGAESEADVVGRNVFTFVDPTSHEGVHERLGRLRTGESVPYVRERFLRAD
                     GSPLDVEVAALPLRYGRRDAVQVIARDITEKVLAHQAVEASEQRYRELVEDVNDILYT
                     VDLQGRFVSLNRSFERSTGYRVEEWIGRPFAELFMPHSVPAATEHFALALGGNERIIH
                     EYDLPSRSGAVVTVEVSWQPRYVDGEVAGTICMARDVTEHRNIARKLEEAKRMSSLGQ
                     VAASLAHEFNNVLMGIQPFVEVIGRNESTTPAVQEALAHITRAVGRGKRASQEILRFA
                     NPKEPQLFSIDASVWLPNLLGQLVAALPSSIALSSSIDPAVQHICGDREHLDQVVTNL
                     VFNARDAIAGNGTIHIAVARITSSTRDPEGNFVRISVCDDGPGIPPHALDRIFEPLFT
                     TKRNGTGLGLAIARRLMEGQGGTLTAENRTEGGSSFHLIVPAAAEPIRSMPLLSLGTP
                     GVTRVLLVEDDLSVGAGLEQLLNSNGYEATWVRVAGDACAAARRTRPQVAIIDVNLPD
                     GNGVDLVPLLRADHENLPIVLSTGHVELDLSSEKTRILSLMKPYQLSELLRAIGTVTA
                     AAA"
     gene            complement(5128..6189)
                     /locus_tag="VNN25_16055"
     CDS             complement(5128..6189)
                     /locus_tag="VNN25_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93096.1"
                     /translation="MPTRRILGLILVLLAFASCYDRQEVDQLGAARRRIKPAPCGGSP
                     AVAGVAGVNPDDSHQVYLGDWIVVSVCNLDVLVHDAEAQQQPITLFIEGLDSGVEPSG
                     MDLESGTLTFILDRTEKNKGIWQQFLYDPLFDPYASMRVSVGIHGDRPLRRADGANMT
                     LKLKKLYVDNTIRVFLALLAAVAIALVVLARRTDMLRDGPPIDGARQPYSLARVQMAW
                     WFFLLVVGYVFIWIVTGERDSIAPSLLGLVGISAATALAAVAVTVAGRRTATTKGFWR
                     DLVTGDTGVVAFDRLQVVVWTLVLGGIFLTAVLWDLTMPEFSATMLALMGISSGTYIG
                     FKLPMKSEPPSAPSDSPAP"
     gene            complement(6523..>8282)
                     /locus_tag="VNN25_16060"
     CDS             complement(6523..>8282)
                     /locus_tag="VNN25_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXH93097.1"
                     /translation="TLRARERTISIARIVFFAIAVIIAIAHFSGWIAIPIVGFIALMV
                     AHDRTITRRRRSEAAARFCERGIERLTGQWQGKGVSGTGYADEHHPFAADLDLFGKGS
                     LFEWLCIAATSAGRTTLANWLLHPGQVRGAEVDERQKAVEELRGLIELREEIAIAAAE
                     VAHDVEEAKLDEWSRAETLLAGRGERIAAIVITALTTAALILGVPSLVTRFVNVTNPG
                     AMPQISLLWSLPLLVMSVVVSLFARRMSARVVQVVGAVERREPALALLAGLLAVLERQ
                     TFTSSRLVALRGRLERNGVIASGQIERLRKLVALLDARRNQFFAPLALLLLWSTHVAF
                     AIERWRVESGAEIVGWIATIAEMEALLSLASFAFEHPSFVMPEIVEGDALFDGDAVGH
                     PLIPDDRRVANDLSIGGELRLLIVSGSNMSGKSTLLRAIGVNTLLALAGAPVCATRLR
                     VSPMAVGASISLNDSLQEGASRFYAEILRIRDILRMTPPLLFLLDEVLGGTNSHDRRI
                     GAAAIVRSLAARGAIGLATTHDLALAQIADELAPRAANVHFEDHIEDGRVIFDYKKRP
                     GVVTKSNALELMRAVGIEV"
BASE COUNT         1545 a         2719 c         2474 g         1544 t
ORIGIN      
        1 gttcagccgg tcgaccatcg tgttgatggt gtccttgagc tcaaggatct cgccgcgcac
       61 gtcgacggta atcttcttcg acaggtcgcc ggaagccacc gcggtcgtca cctcggcgat
      121 gttgcgcacc tggctcgtca ggttgccggc catcgagttg acggagtccg tcaggtcctt
      181 ccacgtgccc gcgacgccct gcacgtccgc ctgcccgccg agctcgcctt ccgtgccgac
      241 ctcgcgcgcc acgcgcgtga cctcggacgc aaaggagcgg agctgatcga ccatcgtgtt
      301 gatcgtgttc ttcagctcca gcacttcgcc gagcgcctcg gctgtaatct tcttcgacag
      361 gtcgccgttc gcaaccgcgg tcgtgacgtc agcgatattg cgcacctggc cagtgaggtt
      421 cgaggccatc gcattcaccg agtcggtcag atccttccac gtacctgcga cgcccggcac
      481 ctcggcctgg ccgcccaacc gcccttccgt tccgacctcc cgcgccacac gcgtaacctc
      541 ggatgcgaac gaacggagct gatcgaccat cgtgttgatg gtctctttca gctcgagcac
      601 ttcgccgagc gcctcggcag tgattttctt ggagaggtcg ccgtttgcga cagccgtcgt
      661 gacctcggcg atgttgcgca cctgtcccgt caaattcgag gccatcgagt tgaccgagtc
      721 cgtcaaatcc ttccacgtgc ccgcgacacc cttgacctca gcctgaccac ccagctttcc
      781 ttccgtgccg acctcgcgcg cgacgcgcgt gacctccgat gcgaacgacc gcaactggtc
      841 gaccatcgtg ttgatcgttt ccttcagctc gaggatctcg ccgcgagcat cgacggtgat
      901 ctgcttcgag agatcgccgt tcgcaacggc cgtcgtgacc gcggcgatgt tgcgtacctg
      961 attcgtcagg ttcgccgcca tgtagttgac cgagtcagtg agatcgcgcc acgtacccga
     1021 aacgccctgc acgtctgcct gaccgccgag tttgccttcg ctgccgactt cgcgcgccac
     1081 gcgcgtgact tccgatgcga acgaccggag ctggtcgacc atcgtgttga tcgtgttctt
     1141 cagttcgagg atttcgccac gtgcttcggc ggagattttc ttggagagat cgccgcgagc
     1201 aaccgcggtc gtgacttcgg cgatgttgcg gacctggccg gtcagattcg aagccatgga
     1261 gttgacggag tcggtgagat ccttccacgt tccggcaaca cccttcacat cggcctggcc
     1321 accgagcttg ccttcggtac caacctcgcg ggccacgcgc gtcacttccg acgcgaacga
     1381 ggcaagctga tcgaccatcg tgttgaccgt cttggcggtg cggagaaact cgcccttcag
     1441 cgggcggccg tcgaactcga tcgccatcgt ccgcgacaga tcgccgcgcg caaccgcgcc
     1501 gatgacacgc gagatctcgt tcgtcggctg cacgagatcg ccgatcaacg agttcaccga
     1561 ctcggtgatg accgcccatt gcccggcggc attcggcacc tgcgcacgct gattgagctt
     1621 cccctctttg ccgacgaccg tcgagatctt ctcgacctct tccgccgtgc gctggttgag
     1681 gtcgaggatg tcattcagtg tgtccgcgat cttcccggcc gcgccggtga actccaccgg
     1741 catgcgcacc ttgaagtcgc ccttcttcat ggaagtgagc gttcgcaata gcattcgctc
     1801 gaagtctcgc ggcgaaagct ccgtcgcttt atcgataccg attcccgtgg tcatgacgcg
     1861 ctggtcctcc gaaggcaggc aactcctagt tgttctgaac tgatcgggcc gaagcagcgc
     1921 tccaggtgga gtgccaggct ctcgacggca aaatgcagat ttcagaccaa tggttgaggg
     1981 cggtatttga ggagcgcgga aaggaattcg gagcagccct caagcttgag cagcctcaac
     2041 gatcaaatgc gggagtcggt agtcgggggt cggagaaaaa gcagagcctt cgttcgaaca
     2101 gatcgcgtgc gtcatccgct tctggggctt cctctccgac ccccgactcc cgaaacccgg
     2161 ctcccgcacc aggcaccgca tcctcgtcgc caaggatgcg gtgccggaac cttttctacg
     2221 cggcgatttt cgcggctttc gctacgagct catcacgaag aacgcggaag gctctcgcgt
     2281 cggcctctcc cgtctccggg tcgaggaaat aggcgacgtc gttgcggaaa ctctcgccct
     2341 cgggattctc gccgttccac tccagcaccg cccactgcac gagcgggttg aagatgttgt
     2401 gatcccaccc gaaggcagcg tgcttcttgc tgtacgcctc ggattttcca agcttctgat
     2461 cggagtctgt tccgccatcg tcgatccggg tcttgtatgc ggcgaacgcc ttggcgtcgc
     2521 gctcggcagt ctgctcatcg acgaagtacg ccacatcgcg gcgcggcgga agggcctgcg
     2581 gccctccgaa cttctctccg acactctgac acttcaccac cagccattga atgaccggcc
     2641 gtccgatctt gtggtcccac tccaaagcaa cgtatggcat gttcgggctc ctgttcccca
     2701 ttcttgatcc ggcgtccagt cgccgggcga gctgcgtaag tgatcccgac agatagttat
     2761 ttccatcttc acgactaaat tattaatatc ttcagccgag gtcgctgcca tatggaatag
     2821 agctgaggac attcttatta tttgcagtca ctacacaatt cacggggagt tcaaatggtc
     2881 ctttctctcg atagtcagag tcaacgccgg tcccgcctgc tcgatgctat gcctgcgatt
     2941 gcatggtcgg cagacgcgca aacgtttcgc ttcacctacg tcaacccggc ggcggaaacg
     3001 ctcctcggct atcccgtcga gcgctggctg gatgagcccg ggttctggat cgagcatctg
     3061 caccctgacg accggcacgt ggcgatgatc tgccacaacg agacgctggc tgcccgcgat
     3121 cacgagctcg tctaccgcat catcgccgcc gacggccgca cggtctggct tcgcgactac
     3181 gtcaacgttc actgcgagga cgggatgccg gtcgagctgt tcggcgtcat ggtcgacatt
     3241 acgcgcgagc gcgaggccga agcggcgtcg cgggaaaacc gcgagaactt tcgccgcatg
     3301 gtcgagctgt cgcccgattg catcggcgtc catgtcgacg agacctacgt ctatgtcaat
     3361 caggctttcg tccacctcct cggtgcagag agcgaggccg atgtcgtcgg ccggaacgtc
     3421 ttcacgttcg tagaccccac atcccatgaa ggcgtgcacg aacgcctggg acgccttcgc
     3481 accggcgaaa gcgtgcccta cgttcgcgaa aggttcctgc gcgccgatgg ctcgcctttg
     3541 gatgtcgaag tcgcggcgct gccgctccgt tacggacgcc gtgatgccgt gcaggtcatc
     3601 gcgcgcgaca tcacggaaaa agtgctggcc caccaggccg tcgaagcttc cgagcagcgt
     3661 tatcgcgagc tcgtcgaaga tgtcaacgac atcctctata cagtcgatct ccagggccgg
     3721 ttcgtctcac tgaaccgtag tttcgagcgg agtaccggct atcgtgtcga agagtggatc
     3781 ggccgcccat tcgcggagct cttcatgccg cattccgtgc ccgcggccac cgagcacttc
     3841 gcgctggcac tcggcggcaa cgagagaatc atccacgagt acgacctccc ttcgcgctcc
     3901 ggagccgtgg tcaccgttga ggtgtcgtgg caaccccgct acgtcgatgg cgaagtcgcc
     3961 ggcacgatct gcatggcgcg cgacgtcacg gagcacagaa acatcgcccg caagctggaa
     4021 gaagcaaagc ggatgagcag cctgggccag gtagcagcat cgctggcgca cgaattcaac
     4081 aacgtgctga tgggaatcca gccgttcgtc gaagtcatcg gacggaacga gtcgaccact
     4141 cccgccgtcc aggaggcgct cgcccatatc acccgcgccg tcggccgcgg caaacgcgca
     4201 tcacaggaga tcctccgttt tgccaatccg aaagagcccc aactcttctc catcgacgcc
     4261 tcggtctggc tgccaaacct actcggccaa ctcgtagcag cgcttccctc ttcgatcgca
     4321 ctgagcagtt cgatcgatcc ggccgtgcag cacatctgcg gcgaccgcga acatctcgac
     4381 caggtagtca ccaaccttgt cttcaacgcc cgtgacgcca tcgccggcaa cggcacgatt
     4441 catattgctg tcgcacgcat cacgtcgtcg acgcgcgatc ccgagggtaa cttcgttcgc
     4501 atttctgtct gcgacgatgg ccccgggatt ccaccgcatg cgctcgaccg gatctttgag
     4561 ccgctcttca cgacgaagcg caacggcacc ggcctcggtc tcgcgatcgc gagacgcctc
     4621 atggaaggac agggaggcac gctcaccgcg gagaatcgca ccgaaggcgg ttcctccttt
     4681 catctgatcg tgccggccgc ggcagaaccg attcggtcga tgccgcttct ctccctcggc
     4741 acgcccggag tgacgcgagt cctgctcgtc gaagacgatc tctccgtggg ggcaggattg
     4801 gaacagctcc tcaactcgaa cggatacgaa gcgacctggg tgcgcgtggc gggagatgcc
     4861 tgcgcggccg cgcgaagaac gcgtccccag gtcgccatca tcgatgtgaa ccttcccgac
     4921 ggcaacggag tcgatctcgt tcctcttctg cgcgccgatc acgagaacct gcccatcgtc
     4981 ctgtctaccg gccatgtgga gctggacctc tccagcgaaa aaacgcgcat cctctcgctg
     5041 atgaagcctt accaactcag cgagcttctg cgggcgatcg gaacggtgac cgcggcggcg
     5101 gcgtgagggt cgccgccgac cgctgaatca cggagctggg gaatcggagg gcgcgcttgg
     5161 cggctcgctc ttcatcggca gcttgaatcc gatatacgtc cccgaactga ttcccatcag
     5221 tgccagcatc gtggcgctga actcgggcat cgtcagatcc cataacacgg cggtcaggaa
     5281 gatcccgccg aggacgagcg tccagacgac gacctgaaga cgatcgaacg cgacgacgcc
     5341 ggtgtcgccg gtgacgagat cgcgccagaa gcccttcgtc gtggccgttc ggcggcccgc
     5401 gaccgttacc gcgaccgcgg ccagtgccgt ggccgcgctg atcccgacga gcccgaggag
     5461 cgagggggcg atcgagtcgc gctcgccggt cacgatccag atgaagacgt agccgacgac
     5521 gaggaggaag aaccaccacg ccatctgcac acgcgccagg ctgtacggtt gccgtgcacc
     5581 atcgatcggc ggaccatcgc gcaacatgtc ggtacggcgc gcaagaacaa ccagcgcaat
     5641 cgcgaccgct gcgaggagag ccagaaagac ccggatcgta ttgtcgacgt agagcttctt
     5701 gagcttcaac gtcatgttcg cgccatccgc acgccgcagc ggccggtcgc cgtgaatgcc
     5761 gacactcacg cgcatcgacg cgtacggatc gaaaagcggg tcgtaaagaa actgctgcca
     5821 gatccctttg ttcttctccg tccgatcgag gatgaacgtc agcgtcccgc tctcgagatc
     5881 catccccgag ggttcaacac cgctgtcgag cccctcgatg aaaagcgtaa tcggctgctg
     5941 ctgcgcctcc gcatcgtgaa caagtacatc gagattacag accgatacga cgatccaatc
     6001 gccgaggtaa acctgatgag aatcgtctgg gttgacgccc gccacacccg ccaccgccgg
     6061 actcccgcca catggcgcag gtttgatccg ccgccgcgcc gcaccaagct gatccacttc
     6121 ctgccggtcg tagcaggagg caaaggccag cagcacgagg atcaggccga gaatgcgacg
     6181 tgttggcatg gagtgcgggt aagtataagg aatgaggcgc gtgtcttgtt tctagatcaa
     6241 atgatcggag ctatcggccc gtatccaacc atcgaggcgg gacgcctcga tggccgcggt
     6301 cgagacgacc gcactcctca gggaggcacg ctcatcgtag ggagtgcggt cgtctcgacc
     6361 gcgggcagtc gggcgtcctc gcccgaatgc ggtcagggcg gaggtcgcaa aatttgtcga
     6421 gatcttttgt ttgcgtgaag taaagcagac tgacagataa atggagcccg caatcgattc
     6481 aaaactcggc gactccactc gctaatctcg atcgtcgctc cttcacacct cgatccccac
     6541 tgccctcatc agctccagcg cgttgctttt cgtcacgacg ccggggcgct tcttgtaatc
     6601 gaaaatcacg cggccgtctt cgatgtggtc ctcgaagtgg acgttggccg ctcgcggcgc
     6661 gagctcgtcg gcgatctggg ccagtgccag gtcgtgcgtc gtggccagcc cgatggcgcc
     6721 tcgggcggcc agactgcgaa cgatcgctgc ggcgccgatg cggcggtcgt gggagttcgt
     6781 gccgccgagg acttcgtcga ggaggaaaag gagcggaggc gtcatgcgca gaatgtcgcg
     6841 aatgcgcagg atctccgcgt agaagcgcga tgctccttcc tgcaatgagt cgttcaggct
     6901 gatcgatgcg cctacggcca tcgggctcac gcgcaggcgc gtggcacaca ccggcgcacc
     6961 ggcgagagcg aggagcgtgt tcacgccgat cgcgcgcagc agcgtgctct tgccggacat
     7021 gttcgagccg ctgacgatga ggaggcgcag ctcgccgccg atgctcaagt cgttggcgac
     7081 gcggcggtcg tcggggatga gcgggtggcc gaccgcatcg ccgtcgaaga gagcgtcgcc
     7141 ttcgacgatc tccggcatca cgaagcttgg atgctcgaat gcgaacgatg ccaacgagag
     7201 cagcgcctcc atctcagcaa tcgttgcgat ccaacccacg atctcggccc ccgactcgac
     7261 ccgccagcgc tcgatagcga aggcgacgtg cgtcgaccaa agcagcagca gagccagtgg
     7321 cgcgaaaaac tgattgcgcc tggcgtcgag aagcgccacg agcttgcgca gccgctcgat
     7381 ctggcccgat gcgatgacgc cgttgcgttc gaggcgtccg cgcaacgcaa ccaggcgcga
     7441 cgaagtgaac gtctgccgct ccagcaccgc aagcaaaccg gccagcagcg ccagcgccgg
     7501 ctcgcgacgc tccaccgcgc cgacaacctg cacaacgcgc gcggacatcc ttcgcgcaaa
     7561 gagagagacg accacgctca tcaccagcaa cgggagcgac cagagcagcg agatctgtgg
     7621 catggcgccg gggttggtga cgttgacgaa acgcgtgacg agcgacggaa cgccaaggat
     7681 gagggcggcc gtggtcaacg cagtgatgac gattgcggcg atccgttctc cgcgccccgc
     7741 cagcagcgtt tccgcgcgcg accattcgtc gagcttcgct tcttccacat cgtgcgcgac
     7801 ttcggctgcg gcgatggcga tctcctcgcg aagctcaatg aggccgcgca actcttcgac
     7861 cgctttctgc cgttcgtcaa cctcggcacc ccttacctgg ccggggtgca gcagccagtt
     7921 cgcgagcgtc gttcttcccg cgctcgtggc agcaatgcac agccattcga acagtgagcc
     7981 ctttccgaag agatcgagat ccgcggcgaa gggatgatgc tcgtcggcgt agcctgtgcc
     8041 ggaaactccc ttcccctgcc attggccggt cagcctttcg attccccgct cgcagaatcg
     8101 cgccgcggcc tcggatcgtc ggcggcgcgt gatcgtccgg tcgtgcgcaa ccatcaacgc
     8161 gatgaaacca acgattggaa tggcaatcca accgctgaaa tgagcaatgg cgatgattac
     8221 cgcgatcgcg aagaaaacga tgcgggcgat ggaaatcgtg cgctcacggg cacgaagcgt
     8281 gg
//
LOCUS       DATLVW010000537         4381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_192119, whole genome shotgun sequence.
ACCESSION   DATLVW010000537 DATLVW010000000
VERSION     DATLVW010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192119"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VNN25_16065"
     CDS             <1..654
                     /locus_tag="VNN25_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HXH93098.1"
                     /translation="RAYADGLKRLLGGVTGISKISVQTGTSHGGVPLPDGSIAQAKID
                     FEVLRETTHICREEYGMAGAVQHGASTLPESVFNHFPESEAVEIHLATGFQNMILDAP
                     TMPQEIKDGIRDFCFEHCLDERKPEQTDEQFVYTTRKKALGPFKRRMWEMDASAKDPI
                     IKELEEKFEFLMEKLGVFGTKDIVAKYVKATPGDLPEYAAASAELTAAAVVDPNEGE"
     gene            complement(910..1113)
                     /locus_tag="VNN25_16070"
     CDS             complement(910..1113)
                     /locus_tag="VNN25_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXH93099.1"
                     /translation="MKQTGTVKWFNDAKGFGFITSEKGEDVFVHFSAIQGNGFRSLAE
                     GAQVEFEVTQGPKGLQAQNVFVV"
     gene            2050..>4381
                     /locus_tag="VNN25_16075"
     CDS             2050..>4381
                     /locus_tag="VNN25_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXH93100.1"
                     /translation="MTANEALLRFAPYRAIAEAVAERLTESPKVEVLAASGGVKDAIA
                     RELLRRSPNGIAGLRIETIETFATRIVNASGELPRVASEAERRLAMRTAVQRIDDPMM
                     ESRGIAAMIERSWRDVRDSGITLAEFERRLRATRAVRNPARSRLLVRAWREYERLIMQ
                     LGAIDPADLLARAAAIIAAGKIPIETQIVAGFYDMTGAQRNVIEALRSIGKLEAIYIP
                     AAGGEAYRFADAFIDRFEAAPPRDHDTPLPQWSVQQFDTRTIELRETCRAAGKLLRDG
                     VPAAAIGIVARTLDPYDIALLNRFAGEEGFAPSAPDKTPLAAHRLGRGIANLLRLRES
                     NFARGEVIELLRDGFEPKVAVDIDALDLATRKARIAGGTSEELKPLARNAAIDSYVAV
                     VAEVEGITPNATMSGSEAADFLATAIARFRIETELDLAAAEAIDEIAATFRRTAKWNV
                     SFDATAILDAFAQESLDAPTPHSPLPTIFCSDVMRFRGRSFEHLFAIRMQDDLFPQRR
                     VDDPLLPDHDRRAMGVRGIGDGRDEERMLFQLLLDGAKTIHFSFAGGDGFAKVLRPSP
                     LLKSFVIARQPERRGELLKNLGSAFVRRRDAAGPAGGTPAFRRASVRQLQLLAKSGTR
                     STFDGYLFAENDDLALRARLASAITSVSPTHLEDFGECPQKFFFKHILGVRDIDDPEH
                     ELQMNARDKGKLDHGILEQFYRGLSEDDFARLAAALPQLDSSVAARIDALVDDAFTAM
                     ARDVPPFNPTMRAIEQTATKRILREFVTHDLADLEAT"
BASE COUNT          831 a         1387 c         1390 g          773 t
ORIGIN      
        1 cgtgcctacg ccgacggact gaagcgactc ctcggcgggg tcaccggcat tagcaagatc
       61 tccgtgcaga ccggcacatc gcacggcggt gttccactcc ccgacggctc gatcgcgcaa
      121 gcgaagatcg acttcgaagt ccttcgcgaa acgacccaca tctgccgcga ggagtacggc
      181 atggccggag ccgttcaaca tggcgcgtcg accttgccgg agtcggtgtt caaccacttc
      241 cccgaatccg aagcggtcga gatccacctc gccaccggct ttcagaacat gatcctcgac
      301 gcgccgacga tgccgcagga gatcaaagac ggcatccgcg acttctgttt cgagcattgt
      361 ctcgacgagc ggaagcccga gcagaccgac gagcagttcg tctacacgac acgtaagaag
      421 gcgctcggac cattcaagcg ccgcatgtgg gagatggacg cgagcgccaa ggacccgatc
      481 atcaaagagc tcgaggagaa gttcgagttc ctgatggaga agctaggcgt cttcggcacg
      541 aaagacatcg tcgcaaagta cgtcaaagca acaccaggcg acctgccgga gtacgcggcg
      601 gcgtccgcgg agctgacggc ggcggcagtg gtggacccga acgaggggga gtgagggatt
      661 cagagatcag agacaaaatc agagacaaga gatcagagat caggcggtgg ccggtgtgtt
      721 tttctgttcc ctgatctctt tccggcggag atcagagacc agagatcagg ctgtgcttgg
      781 tgtgttccgt tccctgatct ctggtctctg ggtctctgat ctcttgtctc tgatctccta
      841 tctctgttcc acacgcaaaa agggcagccc gttcaggctg ccctttcatc gtgatgccgt
      901 cgtgtcgttt tagacgacga agacgttctg agcctgcagg cccttggggc cctgcgtgac
      961 ttcgaactcg acctgcgcgc cctcggcgag cgagcggaag ccgttgccct gaattgcgga
     1021 gaaatgaacg aacacgtctt cgcccttctc cgaggtgatg aatccaaagc ccttcgcgtc
     1081 gttgaaccac ttcactgttc cggtctgttt caaaatgaat ctcctttttt cgatgctgcc
     1141 tgatcggaga ttcgaaacat acgcttctac tctcccctgt taggcaaacc aagtggacgg
     1201 caaaacggca cgaccgcagg ccgaaattcc agagcctgtt gcgagaggtc tgttcgattc
     1261 ggtgagggat aatcgttcga tgacacgatg gcgcgtggga attggccttg cgatccccag
     1321 gctggcgaag gggggctcgg gcggcggtcc aatctccgag gcctgcttta gcgggcgaga
     1381 gaagaccggc tgcacgaaaa cgctgtgtcg ccattctctg tcgcccgctg aagcgggctt
     1441 cgcaaattgg tatggccctc gagacccccg gctgaagccg ggggctacgg atctggtgtc
     1501 tcacgcttgc aaccgccagt tgccgctgaa gtacacacgg gtgcacgcga tctgggggag
     1561 cagaggctgc cgacgcatac agctggacat gaacgttaga tccgtagccc ccggcttcag
     1621 ccgggggtct ggccgggtcc accaattttg cgaagcccgc ttcagcgggc gacagagaat
     1681 tggccttgcg atccccggcc tgaggcaggg gggctccggc ggcggtccaa tctccgaggc
     1741 ctgctttagc gggcgagaga attccggctg cacgaaaacg ctgtgccgcc accattctct
     1801 gtcgcccgct gaagcgggct tcgcaaattg gtatggccct cgagaccccc ggctgaagcc
     1861 gggggctacg gatctggcgt ctggtcacgc ttgcaaccgc cagttgccgc tgaagtacgc
     1921 gcgggtgaac gcgatctgtg ggagccgaga cgacccacgt tttcaggatg gacctggtac
     1981 caggcccatg tcatgtcaca ccggaagggc ttcacaactc gatcatcaga aaaggaccgg
     2041 cgcctcggga tgacagccaa cgaggccctc cttcgcttcg caccttatcg cgccatcgcg
     2101 gaagccgtcg cggaacgact gacggagagc ccgaaggtcg aggtcctagc cgcctccggc
     2161 ggcgtcaagg atgccatcgc acgcgagctc ctgcggcgaa gcccgaacgg catcgcagga
     2221 ctgcgcatcg aaacgatcga gacgttcgcc acacgaatcg tcaacgcgtc cggcgagctt
     2281 ccgcgcgtcg ccagcgaggc cgagcggcgg ctggcaatgc gcaccgcggt ccagcgcatc
     2341 gacgatccga tgatggagag ccgcggcatc gcagcgatga tcgagcggtc gtggcgcgac
     2401 gtccgcgaca gcggcatcac gctggcggaa ttcgaacggc ggctgcgagc gacgcgggct
     2461 gtgcgcaatc ctgcgcgatc gcgcctgctc gtccgcgcct ggcgcgagta cgagcggctc
     2521 atcatgcaac tcggcgccat cgatcccgcg gacttgctcg cgcgcgcagc ggccatcatc
     2581 gcggcgggaa agatcccgat tgagacgcag atcgtagccg gcttctacga tatgaccggc
     2641 gcgcagcgaa acgtgatcga ggcgctgcgt agtatcggca agctcgaagc aatctacatc
     2701 ccggccgccg gcggcgaggc ctaccgcttc gccgatgcct tcatcgaccg tttcgaagcc
     2761 gcgccaccgc gagaccacga cacgcccctt ccgcaatggt cggtccagca attcgacacg
     2821 cgcacgatcg agctccgcga gacctgccgc gccgcgggaa agctgctgcg cgacggcgtg
     2881 ccggcagcgg cgatcggcat cgtcgcgcgt acgctcgatc cctacgacat cgcactgctc
     2941 aaccgcttcg cgggcgaaga aggcttcgcg ccgagcgctc cggacaagac gccgctcgcg
     3001 gcgcatcgcc tcgggcgcgg catcgccaat cttctgcgtt tgcgcgaaag caacttcgcg
     3061 cgcggcgagg tcatcgagct gctacgcgat gggttcgagc cgaaggtcgc ggtcgacatc
     3121 gatgccctcg atctcgcaac gcgcaaagcc cgcattgccg gaggcacgag cgaagagttg
     3181 aagccgctgg cccgcaatgc agcgatcgac agttatgtgg ctgtggtcgc cgaggtggag
     3241 gggatcacgc cgaatgcaac gatgagcggg agcgaagccg ccgatttcct ggcgacggcg
     3301 atcgcgcgct ttcgtatcga gaccgagctc gacctggccg ccgcggaggc gatcgacgaa
     3361 atcgccgcga cgttccggcg cacagcgaag tggaacgtgt cgttcgatgc cactgcaatt
     3421 ctcgacgcat tcgcgcaaga atccttggac gcacccaccc cccactcccc actccccaca
     3481 atcttctgtt ccgacgtcat gcgctttcgt ggacgctcgt tcgagcatct cttcgcgatc
     3541 cgcatgcagg acgatctctt cccgcagcgg cgcgtcgacg atccgctgct gcccgatcac
     3601 gatcggcgcg cgatgggcgt tcgcggcatc ggcgatgggc gcgatgaaga gcggatgctg
     3661 tttcagcttc tgctcgacgg ggcgaagacg attcattttt cgtttgccgg gggcgatgga
     3721 ttcgcgaagg tgttgcggcc gtcgccgctg ctgaagagtt ttgtgattgc gcggcagccg
     3781 gagcggcggg gggagttgct gaagaacctt ggttcggcgt tcgtgcggcg gcgggacgcc
     3841 gccgggccag ccggcgggac gccggcgttc cggcgcgcaa gtgttcgtca attgcaactg
     3901 ctggcgaagt cggggacgcg gtcgacgttc gatgggtacc ttttcgcaga gaacgacgat
     3961 ttggcgctgc gcgccaggct ggcaagcgcg atcacctcgg tctcccccac gcatctcgaa
     4021 gacttcgggg aatgtccgca gaagttcttc ttcaagcaca tcctcggcgt ccgcgacatc
     4081 gacgatcccg agcacgaact gcagatgaat gcgcgcgaca aagggaagct cgatcacggc
     4141 atcctcgagc agttctatcg cgggctgagc gaggacgatt ttgcgcgcct cgccgcggca
     4201 ctgccgcagc tcgacagcag cgttgccgcg cgaatcgacg cgctcgtcga cgacgccttc
     4261 accgcgatgg cccgcgatgt ccctccgttc aatccgacga tgcgcgccat cgagcagacc
     4321 gcgacgaagc gcatccttcg cgagttcgtc acccacgatc tcgccgacct cgaagccacc
     4381 g
//
LOCUS       DATLVW010000538         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_357563, whole genome shotgun sequence.
ACCESSION   DATLVW010000538 DATLVW010000000
VERSION     DATLVW010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357563"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..>2051)
                     /locus_tag="VNN25_16080"
     CDS             complement(362..>2051)
                     /locus_tag="VNN25_16080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH93101.1"
                     /translation="IYDVAAGTSRKVSDLAGGAGSVKWLPDGSGLLVVSDIYPDCGVD
                     PACIKDKTTAEAAATTKAHVMTGLLYRHWKAWQEPTRTHIVYVPLGGGAPRDLTPGAF
                     DAPPFSLGGGDEFDVSPDGKELVYARDTSDHPELSTNSDLFIVPIAGGTAKRITTRAG
                     ADTSPSYSHDGRWIAYRSQARAGYESDLWELWLYDRQSGTAHRVGADFDDWIESVTWS
                     PDSKSMFVIAPQHGRDVLFEVTLDGKFSPIDGYGSTSAVAIAKGGQTLYCSRSGLGGP
                     TEIYAVNKRTSAVQRLTHENDALLTSYRIGMYHDVNWTGADNARIQGFLMTPPGFDQT
                     KKHPAIVLIHGGPQGAWSDSWSYRWNPEMFAARGYVVFMPNPRGSSGFGQKFVEEISG
                     DWGGKAYIDIMNGVDMLAALPYVDGTRIGAAGGSYGGYMIDWILGHTDRFKALVSHAG
                     VYDLKSEYGVTEELWFPEWEFKGNPWDNPELYQRWSPDNFVKNFKTPTLVTHGELDFR
                     VPINQGLELFTALQRRGVPSKMVDFPDEGHWVLKPQNSKLWYQTVGDWFDQWLK"
BASE COUNT          385 a          616 c          643 g          407 t
ORIGIN      
        1 cggccagttc caattcccct ttggccagac gcagatgttc gagcgctgac tgcatgtggg
       61 gttggtcggc gatggcgcga cctgcgacga atcccgaggc gatagcggtg acgataagaa
      121 gggcgatcac gagacgatgc agcttcatgg gtttctcctc ctattcgata atcgacgcgg
      181 catgcgtcga agcgtttacc ggtggggtcc ccctgtagga atcaaccgat agtcgatcag
      241 tcgatgagcg gtcagtgatg agcaaaaaaa gtgcctgacc actgaccaca aaccgactga
      301 catctaatcg acggactatg aatctactga ccactgatct ctgctttacc tgatcactgc
      361 ttcacttcag ccactgatcg aaccaatcac ccacggtctg gtaccagagc ttcgagttct
      421 gcggcttcag cacccagtgc ccttcgtcgg ggaaatcgac cattttcgat ggaacgccgc
      481 gccgctgcag ggctgtgaac agctccagtc cttgattgat cggcacccgg aaatcgagct
      541 cgccgtgcgt gaccagcgtc ggcgtcttga agttcttcac gaagttgtcg ggcgaccagc
      601 gctggtacag ctcggggttg tcccacggat tgcccttgaa ttcccactcc ggaaaccaca
      661 gctcctcggt tactccatac tccgacttca gatcgtaaac gccggcgtgc gagacgagcg
      721 ctttgaagcg gtcggtgtgg ccgaggatcc agtcgatcat gtaaccgccg taggagccgc
      781 ccgccgctcc gatgcgtgtt ccgtcgacgt acggcagcgc cgccagcata tcgacgccgt
      841 tcatgatgtc tatgtaggcc ttgccgcccc agtcacctga gatctcttcg acgaacttct
      901 gtccgaagcc gctggagccg cgcgggttgg gcatgaacac gacgtatccg cgcgccgcga
      961 acatctcggg gttccagcga tacgaccagg aatcgctcca tgcgccctgc ggcccgccgt
     1021 ggatcaacac gatggccgga tgctttttcg tctggtcgaa gccgggaggc gtcatgagaa
     1081 aaccctggat gcgggcgttg tcggcgccgg tccagttgac atcgtgatac atgccgatcc
     1141 gataactggt gagcagcgca tcgttttcgt gcgtcaaacg ttgcacggca ctcgtgcgtt
     1201 tgttgacggc gtagatttcg gtcgggccac caaggccgga gcgcgagcag tagagcgtct
     1261 ggccgccctt cgcaatggcc acggcactcg tcgatccgta gccgtcgatt ggcgagaact
     1321 tgccgtcgag cgtcacctcg aacaacacat cgcggccgtg ctgcggagcg atgacgaaca
     1381 tcgatttcga gtcaggcgac caggtgacgg actcgatcca atcgtcgaag tcggcgccca
     1441 cgcgatgtgc agttccggac tgccggtcgt agagccaaag ctcccaaagg tcggattcgt
     1501 agccggcacg cgcctgcgag cggtaggcga tccagcggcc gtcgtgcgag tagctcggcg
     1561 acgtgtcggc accggcgcgg gtcgtgattc gtttcgccgt tccgccggcg atcgggacga
     1621 tgaagaggtc ggagttcgta gagagctccg gatggtcgga ggtgtcgcgg gcgtagacga
     1681 gctctttgcc gtcaggtgac acgtcgaatt cgtcgccgcc tccgagagag aacggtggcg
     1741 cgtcgaacgc gccgggcgtg aggtcgcgtg gtgcgccgcc accaaggggg acgtagacga
     1801 tgtgtgtgcg cgttggctcc tgccaggctt tccagtggcg atagaggaga cccgtcatca
     1861 cgtgcgcttt cgtcgtcgcg gctgcctcgg cggtggtctt gtccttgatg caggcaggat
     1921 cgacgccgca atcggggtag atatcggaaa cgacgaggag ccccgaccca tccggtagcc
     1981 acttcacgct tcctgcgcca ccggccagat cgctcacttt ccgcgaagtg ccagcggcga
     2041 cgtcgtagat g
//
LOCUS       DATLVW010000539         4086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_272598, whole genome shotgun sequence.
ACCESSION   DATLVW010000539 DATLVW010000000
VERSION     DATLVW010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272598"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VNN25_16085"
     CDS             <1..412
                     /locus_tag="VNN25_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HXH93102.1"
                     /translation="MLPFIVLMFIFIVPSIVAEAADNDAMRNIGGGMAACVWCIIFPL
                     GLAMALWLPAALMFAAVERRFGAAFEFAQIAAFIKANIGNYLLAFVVWLVVRMIVPFG
                     MILLCVGIFLTSFWSLVVAAYSFGQVWRLAPARR"
     gene            409..861
                     /locus_tag="VNN25_16090"
     CDS             409..861
                     /locus_tag="VNN25_16090"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HXH93103.1"
                     /translation="MKRDTKIVAATLVAISGAILAASIACTAAIANGASMRWRLLFRL
                     FCHGIPERCLYLWNTPMPICARCTAIYVGLALSALLFRILPRMKEQTARYILYGAVLP
                     MAIDGLTQLVHLRLSSNPLRIETGLLAGLAFGVWALSAIENHEIVTIP"
     gene            923..1729
                     /locus_tag="VNN25_16095"
     CDS             923..1729
                     /locus_tag="VNN25_16095"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH93104.1"
                     /translation="MAFQGSLKELPLPDIIQLVSVSGKTGKFTLSRDHDRGYIYLKNG
                     QMVHATVGELVGEEAIYALAIWNQGDFQFSPNEEPDRQTITKSNTNLLMEAARRLDEW
                     RVLSKKVPSIDYVPELQVRESRHEQITLNPQEWLLITRIDGQRSIADIGKVLNISSFE
                     VAKILYGMITGELVALKKKPEFEHDAESGELVDLAARIRSIAEEFIGNSAHKTIEKHF
                     LRALDGIMSGDGEHAVDAMVSEFEKTASLLRGLAVTEQLRSRITELRRAS"
     gene            1980..2351
                     /locus_tag="VNN25_16100"
     CDS             1980..2351
                     /locus_tag="VNN25_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93105.1"
                     /translation="MRGARPEELQHAANPMLTARLSESELAEWFPVVFEEITDPWATP
                     EPSKGALIRLDSDEYIVIYWGYDSSELTVETPNVIPDKHVLASLFREVPQLESRILWQ
                     RPDERPSGDLSSPECVSQVDH"
     gene            complement(2462..>4086)
                     /locus_tag="VNN25_16105"
     CDS             complement(2462..>4086)
                     /locus_tag="VNN25_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93106.1"
                     /translation="PHTPTNCYLLLSPCTVTRLLPLFLALVIVSPLGAQTEQPLRIGT
                     ITVRPLDVYSPDEAVHGHLYRLADQLHIETRKSVIEKFLLFHEGDVYSAERLAETERN
                     LRALQFLKSASVEASAPHDGVVDVVVTTQDSWSIAPETQAGSKGGKSTVGATLSETNL
                     LGLGKDLELGWNRGVDRTRVGVTYNDPAFFAPYWKGAFAYSTNSDGYDHNLAIVRPFY
                     SFATPWSTELSFSGFRQFDHLYANGLESERFQQKHREIVASYGVALDPNDNQAHRIVA
                     GFHAIDDSFTIMKHSSALALPSGREFRYLFVRYESTANDFVKLNFVNKDVRYEDFNLG
                     RQYSIEAAVSPRNLGADHTSSFARVAGADGKWLGNGFVMPAFAVSSRLDGGLQNSIAT
                     SSLLFVRRGGRASYPTTFLSRVVFNSGWRMDAEDQFFADGLTGLRGYRAHAFAGTRSL
                     VINVEERLYLGREILQLASPGIVAFMDAGNATTGGVTRLMNLKSDIGIGLRLGLPRTP
                     KNLLRVDLAYALNRDPLGRKGWLVAFSSGQAF"
BASE COUNT          782 a         1223 c         1274 g          807 t
ORIGIN      
        1 catgctgccg ttcatcgtgc tgatgttcat cttcatcgtt ccgtcgatcg tcgcagaggc
       61 cgctgacaac gacgcgatgc gcaatatcgg cggcggaatg gctgcctgcg tctggtgcat
      121 catcttcccc ctcgggctgg cgatggctct ctggctgccg gccgctctca tgttcgccgc
      181 cgtcgagcgc cgtttcggcg ccgcattcga gttcgcgcaa attgccgcgt tcatcaaggc
      241 caacatcggc aactacctcc tggccttcgt ggtctggctc gtcgtccgga tgatcgttcc
      301 gttcgggatg atcctgctct gcgtagggat cttcctcacc tcgttctggt cgcttgtcgt
      361 ggccgcctac tcgtttggac aagtctggcg gctggctcct gcgcgccggt gaagcgcgac
      421 acgaaaatcg tcgcggcaac gctggttgcc atctccgggg ccatccttgc ggcgtcgatc
      481 gcctgcacgg ctgccatcgc caacggcgcg tcgatgcggt ggcgccttct cttccgcctt
      541 ttctgccacg gcatcccgga gcgttgtctc tatctctgga acacgccgat gccgatctgc
      601 gcccgctgta cggcgatcta tgtcggactc gctctctctg ctcttctctt tcggatactc
      661 cctcgcatga aggagcaaac cgcgcggtac atcctctacg gagccgtgct gccgatggcc
      721 atcgatggcc tcacccagct cgtgcatttg cggctcagca gcaatccgct acgcatcgaa
      781 accggtcttc tggctggtct tgcgttcggt gtgtgggcgt tgagcgcgat agaaaatcac
      841 gagatcgtta cgattccttg acctcattcc aaccgtgggt tacgcttcaa agccgcggct
      901 ccacataatc gaaatcacta aaatggcctt ccagggatcg ctgaaagagc ttccgctgcc
      961 cgacatcatc cagttggtca gcgtatccgg caagacgggg aagttcacgc tttcgcgcga
     1021 ccacgaccgc ggctacatct atctgaagaa cggtcagatg gtgcacgcca cggtggggga
     1081 gctcgtcggc gaggaagcga tttatgcgct ggcgatctgg aatcagggcg actttcagtt
     1141 cagcccgaac gaagagccgg accgccagac gatcacgaaa tcgaacacga acctgctgat
     1201 ggaagcggcc cgccggctcg atgagtggcg cgtcctttcg aagaaggtgc cttcgatcga
     1261 ctacgtcccc gagctgcaag tgcgcgaatc gcggcacgag cagatcaccc tgaatccgca
     1321 ggaatggctg ctcatcacga ggatcgacgg gcaacggtcg atcgccgaca taggcaaggt
     1381 gctgaacatt tcatcgttcg aggttgccaa gatcctctac ggaatgatca ccggcgagct
     1441 ggtcgcgcta aaaaaaaaac ctgaattcga gcacgatgca gagagtggcg agctagtcga
     1501 cctggccgcg cgcatccgat cgatcgccga ggagttcatc ggcaactccg cccacaaaac
     1561 gatcgagaag cactttctcc gcgccctcga cggcatcatg agcggcgacg gagagcacgc
     1621 agtcgacgcg atggtctcgg agttcgagaa gaccgcctcc ctccttcgcg gattagcggt
     1681 caccgagcag cttcgctcgc ggattacgga gctgcggaga gcttcgtagc gcggcgttag
     1741 gcttgttttc agatctggac cctcttcaaa ccattggagc gatcaggccg aatccaacca
     1801 tcgaggcggg acgcctcaat gcccgcggtc gagacgaccg cactcctctg tgctgccaga
     1861 caacgtgcgt cgtaaggagt gcggtcgtct cgaccgcggg cactcgggcg tcctcgcccg
     1921 agtgcggtca gggcggaggt ccccgttgtt gttacgatga gaagagattt ggagtaagca
     1981 tgcgcggcgc cagaccagaa gaactgcaac acgcggccaa tccgatgcta acggctcgct
     2041 tgtcggagtc ggagctcgcc gagtggtttc cggtagtgtt tgaggaaatc actgatccgt
     2101 gggctacacc cgagccgtcg aaaggtgcgc tcatcagact ggattccgac gaatacatcg
     2161 tcatttattg gggctatgac tcgagcgagt tgacggtgga gacgcccaac gtgattccgg
     2221 acaaacacgt actggcctcg ctctttcgtg aggtgccgca gctggaatcg cgaattctct
     2281 ggcaacggcc cgacgagcga ccttctggcg acctttcgtc acctgaatgc gtctcgcagg
     2341 tggaccactg atcagagaca agggggaacc agagatcaga gaccaggata atcgattcgt
     2401 ctcttttctc cattctttgt caccggcact tgcctccgat ctctgatcgc tgatctctga
     2461 cctaaaacgc ctgcccgctc gagaacgcca ccagccatcc cttccgaccg agtggatcgc
     2521 ggttcagcgc gtaggccagg tcgacgcgca gaaggttctt tggcgtgcga gggaggccga
     2581 ggcggaggcc gatgccgatg tcgctcttga ggttcatcag ccgcgtgacc cctcctgtcg
     2641 tggcgttgcc ggcatccatg aaggctacga ttccgggcga ggccagttgg agaatctcgc
     2701 ggccgaggta gagacgctct tcgacgttga tcacgagcga gcgcgttccg gcgaaggcat
     2761 gggcacgata tccccgcagc cccgttaacc catccgcgaa aaactgatcc tcggcgtcca
     2821 tcctccaccc gctattgaag acaacgcggc tgaggaatgt cgtcggatag gaggcccgcc
     2881 ctccgcgccg cacaaacagc agggaagacg ttgcgatgct gttctgcaaa ccaccgtcga
     2941 gacgtgacga gaccgcgaac gccggcatga cgaagccgtt cccgagccac ttgccatcgg
     3001 ctccggccac gcgcgcgaac gatgaggtgt ggtcggctcc gaggttgcgc ggcgagacgg
     3061 cggcctcgat cgagtactgt cgcccgaggt tgaagtcctc gtagcggaca tccttgttca
     3121 cgaaattcag cttcacgaaa tcgttggcgg tgctctcgta gcggacgaac agatagcgaa
     3181 actcgcggcc tgagggaagg gcaagcgcac tgctgtgctt catgatcgtg aacgagtcgt
     3241 cgatggcatg gaagccagcc acgatgcgat gggcctggtt gtcattcgga tcgagcgcca
     3301 cgccgtagct ggccacgatc tcgcgatgct tctgctggaa gcgctcgctc tcgaggccat
     3361 tggcgtagag gtgatcgaac tgccggaagc cggagaacga aagctcggtc gaccatgggg
     3421 tcgcgaacga atagaaagga cggacgatgg ccaggttgtg gtcatagccg tcggagttcg
     3481 tcgaatacgc aaacgcgcct ttccaatagg gcgcgaagaa cgccgggtcg ttgtaggtca
     3541 ccccgacgcg tgtcctgtcc acgccgcggt tccatcccag ctccagatcc ttgccgagcc
     3601 cgaggaggtt ggtctccgac agcgttgcgc cgacggtgct cttgccgcct ttgctgcccg
     3661 cctgcgtctc cggcgcgatg gaccaggagt cctgcgtcgt gacgacgacg tcgacgacgc
     3721 catcatgcgg agcggaggct tcgacggagg ccgacttcag gaactgcagg gctcggagat
     3781 tgcgctcggt ctcggcaagc cgttccgcgc tgtagacgtc gccttcgtga aagaggagga
     3841 acttctcgat gacggacttg cgtgtctcga tgtggagctg gtcggcaagc cggtagaggt
     3901 gcccgtggac cgcttcatcg ggcgagtaga cgtcgagcgg acggacggtg atcgttccga
     3961 tgcgaagagg ctgctccgtc tgcgcgccca gcggggagac gatgacgagt gcgaggaaga
     4021 gcgggaggag gcgtgtcacc gtgcatggcg agagtaacag ataacagttg gtgggggtgt
     4081 ggggag
//
LOCUS       DATLVW010000540         3754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_67553, whole genome shotgun sequence.
ACCESSION   DATLVW010000540 DATLVW010000000
VERSION     DATLVW010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67553"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..2208)
                     /locus_tag="VNN25_16110"
     CDS             complement(154..2208)
                     /locus_tag="VNN25_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXH93107.1"
                     /translation="MLSGTTTETPSRRLGLRPKFILAFALQTLVSAMLIVGLEQWRVR
                     TVIRDELVADADELSHTVAVAAANAAKTSRVEDLRAITSDLKSRTWIDYADFVTADGK
                     ILASQGTAPDSLSLTPGRSQFVTKDGDALHVFVVPVAGDTTLFRLVANERGALSITQQ
                     LRWSNLGVVLLALLIAIGLALLSSRFIIRPVLDLADHARSLAEGDLTQRATVQSSDEI
                     GDLAEAFNAMAGNLERTITKLVRSQSQLKSVVENVGMQSRNVAERVSEQRSMIDNNYH
                     SIDQLNNGVRKITDNVEALSASSEETSSSMLEMVASIEEVSRHTDTLFNSVEETASAT
                     SQMVSSISEVDQNVDYLANFVTDTSSSMVEMSASIAQVEANAARSYDLALAVADAAES
                     GMKAVRETIDGMEQIRRSVAESNAVITRLGDRSIAIGKILNVIEDIAEQTNLLALNAA
                     ILAASAGEHGKGFSVVAAEIRDLSERTASSTREIANLIRSVQEEVGNALRSMSSGTKL
                     VEDGVSLSHEAGKALNNILDSSTKASGMGKEIAAGTREQTAGSENITRAIDRLQELLS
                     QINGATTQQAQGSDHIMNAVESMREVTKYVRQAMTEQRSGSSMISASAEKMIDMIHEI
                     FQVATNQAAESEKIVATMEQVRALAEGSGSYAAEMGESLTLLSDAIRGLDEEIRRFRV
                     RG"
     gene            2256..3188
                     /locus_tag="VNN25_16115"
     CDS             2256..3188
                     /locus_tag="VNN25_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH93108.1"
                     /translation="MPLIRCAKCGQAYDVPGVIAVRLPNSIATCHCGEWISGSKAALL
                     ARLMNPGEIKEIDLEPYRVERSGDTPVAPVAAPKPPGQPRSIRIIARGAKEPVNRVFT
                     IRNHSLWIGRKGCHVELAESDLSLRHCSISLQGDDLIARDADSHMGTFLDGQRITETP
                     ISDGTHLLRLGSALVSIEPTGEQGVVVEPIRGDAEAPIEEAELRHKITQRTPVPATRP
                     VLICIDGALNGQEFEIPPSGLVVGREGHVRVPDEFLSRRHFEVAPDADGSIHVRDLGS
                     RNGTFLNTLPAHDTRVKSGDEIRAGINRFRIEHR"
     gene            3282..>3754
                     /locus_tag="VNN25_16120"
     CDS             3282..>3754
                     /locus_tag="VNN25_16120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH93109.1"
                     /translation="MSSFAEDAAGRIVRSLSALLHKELSLVDPASEDGAQSFVLTPTL
                     AVRDDDAFNTSGRDLVREVASVVRFAGERDGRVRELERRLAEMESDNLDLVMKNRTLA
                     EISARDSLTGLYNRWYVMEKIDSEMNRSLRHGCPVSVLMLDIDHFERVNDAFGHAA"
BASE COUNT          681 a         1143 c         1196 g          734 t
ORIGIN      
        1 gaccgcgtca ccgatcaccg catcggtctc accgtccaca acctacccga catcatgaac
       61 ggaaatctcg acgttctcct cgagccactc ctcggccact tccaggcgga gaaactgcgc
      121 gaggcggtcg agacgcgctg accggaagaa cgatcagccc cgcacgcgaa agcggcggat
      181 ctcttcgtcc agtccgcgga tggcgtcaga gagaagcgtg agcgattcac ccatctcggc
      241 ggcgtagctc ccgctgcctt ccgcgagcgc gcgtacctgc tccatcgtgg cgacgatctt
      301 ctcgctctcg gcagcctgat tggtggctac ctggaagatc tcgtgaatca tgtcgatcat
      361 cttctcggct gaggcggaga tcatcgacga gccggagcgc tgttcggtca tagcctggcg
      421 cacgtacttg gtcacctcgc gcatcgactc gacggcgttc atgatgtggt ccgagccctg
      481 cgcctgttgg gtcgtggcgc cgttgatctg gctgagaagc tcctgcaacc ggtcgatggc
      541 ccgcgtgatg ttctcgcttc ctgccgtctg ctcgcgcgtt ccagcggcga tctcctttcc
      601 catgcccgac gctttcgtag atgagtcgag aatgttgttc aacgccttcc ctgcctcgtg
      661 cgaaagcgac acgccgtctt cgacgagctt ggtgcctgaa gacatcgagc ggagcgcgtt
      721 gccgacctcc tcctgcacag agcggatgag attggcgatc tcacgagtcg aggatgcggt
      781 gcgctcggag agatcgcgaa tctcggcggc tacgacggag aagcccttgc cgtgttcgcc
      841 ggccgatgcc gcaaggatcg cggcgttcag cgcgagaaga ttcgtctgct cggcgatgtc
      901 ttcgatcacg ttgagaatct tgccgatggc gatcgaccga tccccaagcc gtgtgatgac
      961 ggcgttggac tcggcgacag agcggcgaat ctgctccatt ccgtcgatcg tctcgcgcac
     1021 cgctttcatc ccggattcgg cagcgtcagc gaccgcgagc gcaaggtcgt acgaccgggc
     1081 cgcgttggct tccacctggg cgatcgatgc gctcatctcc accatcgagg atgaggtgtc
     1141 ggtcacgaag ttggccaggt agtcgacgtt ctgatcgacc tcgctgatcg aactgaccat
     1201 ctggctggtg gcggaggcgg tctcctcgac cgagttgaaa agcgtgtcgg tgtgccggct
     1261 gacctcttcg atcgaagcca ccatctcgag catagaggac gacgtttctt ctgaggaggc
     1321 ggacagcgcc tcgacgttgt cggtgatctt gcgaacgccg ttgttgagct gatcgatcga
     1381 gtggtagttg ttgtcgatca tcgaccgctg ctcgctcacg cgctccgcca cattgcgcga
     1441 ctgcatgccg acgttctcga cgacggactt gagttgggat tgcgagcgaa ctagcttcgt
     1501 gatcgtcctc tccaggttgc cagccatggc gttgaaggcc tcggcaaggt cgccgatctc
     1561 gtcgctggac tgaacggtgg cgcgttgcgt gagatctccc tcggccaggc tgcgcgcgtg
     1621 gtcggcgagg tcgaggacgg ggcgaatgat gaagcgcgag gagagcagcg cgaggccaat
     1681 ggcgatcagt agggccagaa gcacgacgcc gaggttcgac cagcggagtt gttgcgtgat
     1741 ggaaagtgcg ccgcgctcgt tcgcgaccag ccggaaaaga gtggtgtcgc cggcgaccgg
     1801 gaccacaaag acgtgcagcg cgtcaccgtc tttggttacg aattgcgaac gccccggcgt
     1861 gagggagagc gaatcaggcg ctgtcccctg cgacgcgaga atcttcccat cagcggtgac
     1921 gaagtcggcg tagtcgatcc aggttcgtga tttgaggtcg ctggtaatcg cgcgaagatc
     1981 ctccacgcgc gatgttttcg ctgcattcgc cgcggcaacg gccacggtgt gcgacagctc
     2041 atcggcgtcg gcaaccaact cgtcgcggat gaccgtgcga acacgccatt gctcgaggcc
     2101 gacgatgagc atcgcgctga cgagcgtctg caacgcgaac gcgagaatga attttggccg
     2161 gagaccgagg cggcgactgg gagtttctgt ggtggtgccg gagagcatgc gaatgagcgg
     2221 aagtgtagcg tatggcgctg cgctatcatc cgcgcatgcc tctaatccgc tgcgcgaaat
     2281 gcggacaggc gtacgacgtc ccgggtgtga tcgccgttcg cctccccaat tccatcgcca
     2341 cgtgccattg cggtgagtgg atttcaggta gcaaggcggc gctgctggcg cgcctgatga
     2401 atccgggcga aatcaaggag atcgacctcg agccatacag agtggagcgg tctggcgaca
     2461 ctcctgttgc accagtcgcg gccccaaagc cgcccggaca gccacgatcg atccgcatca
     2521 tcgcccgcgg tgcgaaggag cctgtcaaca gggttttcac aatccgaaat cactcactct
     2581 ggatcggccg gaaaggttgt cacgtcgagc tggctgagtc ggacctgtcg ctccgtcact
     2641 gctcgatttc gcttcagggc gacgacctga tcgctcggga tgctgattcg cacatgggga
     2701 cgtttctgga tggccaacgg atcacggaga ccccgatcag cgacggaacg catttgctgc
     2761 gtctgggctc cgcgctcgtc agcatcgaac ctaccggcga gcagggcgtt gtcgtagaac
     2821 cgattcgcgg ggatgcggaa gcgcccatcg aagaggcgga gctccgacac aagatcacgc
     2881 agcgaacgcc ggtgccggcc acccgtcccg tcctgatctg catcgatgga gcattgaacg
     2941 gtcaagagtt cgagattcca ccgtcagggc tcgttgtcgg ccgcgaaggt cacgttcgcg
     3001 tgcctgacga gttcctgtcg cggcgccatt tcgaggtcgc tcccgacgcc gatgggtcga
     3061 tccacgtacg cgacctcggc agccgtaacg gtactttcct gaacacgctg cccgcgcacg
     3121 acacgagggt caagagcggc gatgagatcc gcgccggtat caaccgcttt cgcatcgagc
     3181 acaggtaact tccgtcgttc cgtcgtcacc attatcggag cttgacggag gtcgctctcc
     3241 ctacgcatac tccgcgcgat ccaaacaagg gagtgtgtga gatgagctcg ttcgccgaag
     3301 acgcggcggg ccgcatcgtg cggtcgctgt ctgcgttact ccacaaggaa ctttccctgg
     3361 ttgatcccgc gtccgaggat ggcgcgcagt cgttcgtgct gacaccgacg ctggcggtgc
     3421 gagacgacga tgccttcaac acctccggac gagacctcgt tcgtgaagtg gcctcggtcg
     3481 tgcgcttcgc cggcgagcgc gatggacgcg ttcgcgagtt ggaacgtcgt ctcgccgaga
     3541 tggagagcga caatctcgat ctggtcatga agaatcgcac gctcgccgag atctcggcgc
     3601 gtgactcgct gacaggcctt tacaaccgct ggtatgtgat ggagaagatc gacagcgaga
     3661 tgaaccggtc gctgcgtcac ggctgcccgg tctcggtcct catgctcgac atcgaccact
     3721 tcgagcgcgt taatgacgcg tttggccacg ccgc
//
LOCUS       DATLVW010000541        13053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_272911, whole genome shotgun sequence.
ACCESSION   DATLVW010000541 DATLVW010000000
VERSION     DATLVW010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272911"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VNN25_16125"
     CDS             complement(<1..288)
                     /locus_tag="VNN25_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008183368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HXH93110.1"
                     /translation="MVSRKNAAVVFRRRAGQYEKQLTGYRAFIPAPLPPVPMVRVEGE
                     LQALLSKADLALGRLDGSIQTLPNPDLLVLMYVRKEAVLSSQIEGTQSSLQD"
     gene            470..2545
                     /locus_tag="VNN25_16130"
     CDS             470..2545
                     /locus_tag="VNN25_16130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011611227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HXH93111.1"
                     /translation="MLIKEATATPIDYLRTAVGRDDATFRDEQLECIEAAVRGERLLV
                     VQRTGWGKSIVYFIATRMLRDRGAGATLIVSPLLSLMRNQLAAAARIGLRSATINSAN
                     TEDWDAVIRAMRRNEIDVLLISPERLANDAFRQNVLVPIGGNIGLFVIDEAHCISDWG
                     HDFRPDYRRISQLFKLLPPSLPVLATTATANNRVVDDVMAQLGNVSISRGPLVRKSLR
                     LQNILLPDQASRLAWLAQHLPTLPGSGIVYTLTVRDAERVAEWLRLRGIDAVAYHADS
                     ADREMLEARLLRNDLKVLVATVALGMGFDKPDLGFVVHFQRPGSVVHYYQQVGRAGRA
                     VDEAFGILLGGAEDDEIIDFFIRQAFPPEGDVLDVLEALNCADDGLSVTQIESFVNLP
                     FGRIERTLKLLATETPAPIRKEGSKWYRSPVPYVFNAGKVQQLTLIRRREQQRMQEYM
                     RSTDCLMQFLAAELDDPFAAACGKCANCAGRVVSAEVPIEGVIAANEFLQRTRVTIEP
                     RKLWPVAALVKDGFKGKIAPFQNEPGRALSLWEDGGWAELVRRGKYEDDRFDDSLVDA
                     CAALVADWKPDPRPEWLTCVPSKRHPQLVPNFAQRLAARLGIPYRAAIVKSADNLPQK
                     QRENSVQQARNLDGAFAVDRAAMMPGPVLLIDDMTDSGWTLTVTGALLRRARCPAVFP
                     LVLADASRS"
     gene            2545..3540
                     /locus_tag="VNN25_16135"
     CDS             2545..3540
                     /locus_tag="VNN25_16135"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXH93112.1"
                     /translation="MEDVTQEQQVSRDTQAVLLLCSRFAPREQVEPLDTREFSRVEDL
                     LQQRSLRPGDLLDDADLDWAASGLDATRIAQLLDRAMGLGLATERWANGGLWVISRSD
                     DDYPARLLQHLGRSAPPLLWGVGERSLPAFGGIAIVGSREIDDAAAKWAEDVAAAFAR
                     EEITVISGGARGTDQIATAAALNEGGRVIAVLPEGLGRPSVVGHYREAVVDGRLLLLS
                     PVYPDAGFTVGNAMGRNKVIYGIAEAAVIARADANSGGTWAGAEAELKREDAIPVFVR
                     SEGEVADGNQALIALGAREFPAQPWSDLRALLTTSRTSAEAREKPAPGQRSLFER"
     gene            complement(3721..6759)
                     /locus_tag="VNN25_16140"
     CDS             complement(3721..6759)
                     /locus_tag="VNN25_16140"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXH93113.1"
                     /translation="MIRPALLLLASSLFCAPLGAGQALFRNASIDYGSAAVFDMKAGD
                     INNDGKPDAVLLEKLLPSNNWVLLTLAGNGDGSFRTPVSTPITTPGARIALGDLDGDG
                     KLDLVLSGQDTRLETYHGNGDGSFTLISSQIANIIGFSPLALTDLDGDGKLDLIAVTR
                     VDANNQTLVTFRGIGNGTFAAGISQPGPFIQSIDNIESGDYNGDGRADVIESSFYELR
                     LATGNGNGSVNGPTTISHDPFARTTAGGDFNGDGKRDIVEISRLDGTALVHLGNGNGT
                     FTDGAKYVVGGVYSGVAADFDGDGKLDVVAGGNDVVTVMRGNGDGTFAVRAYVANSGP
                     LAVADFDGDHHPDVLAVSSPGLLLLHGNGDGTLRAYRKTFVRPAPAPFYNSRTIPKGL
                     AVADFNGDGKPDVVTLVDQIIVMLTSGDGLGPPVALALPPGITAASSLATGDVNGDGK
                     SDIVVAGSKLWTYLSNGDGTFTAAAPTAITSLTSVFLADVNGDGKLDVLLGNFLFNGQ
                     LLLGNGDGTFALPTTLPGNPGQIADFNGDGRPDFVSTDINGMTIYLNNGSGVFSAGGH
                     VSNTVGAIAVGDFNGDGKADLVEEQYNNTVRIRLGNGDGTFTDLQPFLLGSDLVLASS
                     PANAVAADFDGDGKLDLAISNRVYLGKGDGTLRARVPCVVSRSWSYLAAADIDGNGSA
                     DLLLLDDQNDAVNTLLTRTAAGGATPLPLTLTPSLSTLHFQEPVALTTTATTSSTFLP
                     VGGVTITVDGVFNGFANWNGNTALMTLVPRIIGDHQTIAAEFVGDDIFPPASVSVVRS
                     VLKLDSVVVIEAFPSPSQLGQTTTITARIFAKQADAGPVQPSGTLTIRDGDTVLLSKA
                     YDANHPPALTYKFTTVGTHALAGEYSGDANHTADTETFSQVVTKAIPLVGLSGTPASP
                     IMAGQPLTLKATTFASFSGNGGTMTFREGGIDVGTAPTVGNVASITIQPSAGTHSYTA
                     IYSGNADLDTATTNFTLDYTVLPLPCTTPGGCGRHHAAHP"
     gene            complement(6808..7347)
                     /locus_tag="VNN25_16145"
     CDS             complement(6808..7347)
                     /locus_tag="VNN25_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93114.1"
                     /translation="MLYADESRFREAFNIIEEYIERRYSVQVVISDVTDPFTGDLDGE
                     SIAVDYDQSSEDALFIIAHLLGHTVQWNTNPRAREIGTTTVTAATEEFLGELAAYEKQ
                     ACRYSLQLFHDAGILDLDQWVSDFAACDSAYLMHFYRTGKKLPFRSFWREGMPLIEPL
                     AIPPFQPSRWLARSGGVVL"
     gene            complement(7458..9062)
                     /locus_tag="VNN25_16150"
     CDS             complement(7458..9062)
                     /locus_tag="VNN25_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93115.1"
                     /translation="MFFPRKWRAVVAAAFLITAAPLAAQEMEHHHDGPADERLGTVDF
                     PISCAGSQAAFTRAVALLHSFWYEEAEKAFRVIAVKDPRCGIAWWGVAMSNYHPVWPS
                     PYSPEELARGVAAAEKAKAAGAQTPREQAYIDAIVTFYRGADKLDTVTRARAYEAEME
                     KLTARFPDDDEASIFYGLALVAHGMSVPTDKTYAYQKRAAAIFNRLLDRHPDHPGITH
                     YLIHSFDYPALAPLALKAANTYAKIAPSSPHALHMPSHIYVRLGMWKETIDSNLASAK
                     AAREHAEKTHPGTASFDSLHAWDYLAYAWLQQGQDEKVRELVDQIATIKALNVENFAG
                     YYAAAAVPARYALERRSWSEAAALTVRPAAFAWERYAYAEALTHFARAVGAARSGDVA
                     HAHEAVARLEQLRQKLIEQKNGYWADQIEVQRRVADAWIAHAEKRDDGAFTLMRSAAD
                     LEDSMDKSPVTPGSIIPAREMLGDLLLELGQPDKALEAYERSLADSPNRLNGLTGAAR
                     AAQLKGDRGKAAGYYVQIAKLLGSGG"
     gene            9162..9614
                     /locus_tag="VNN25_16155"
     CDS             9162..9614
                     /locus_tag="VNN25_16155"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HXH93116.1"
                     /translation="MRTRALLAAAILASLLPASSAFAFCFGTIHVHVFNDTNRNGVLD
                     AGEAAVPGVAVQLDQLGDGTVEETLTTNANGDVDFLAPAVVSYQVRIVAPAGMVQTSV
                     TPPTFPITCDGVTNVSFGLIQAIPAVSPMLLALLAISLMAVAVVTLRR"
     gene            complement(9621..12569)
                     /locus_tag="VNN25_16160"
     CDS             complement(9621..12569)
                     /locus_tag="VNN25_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXH93117.1"
                     /translation="MLRTLAGHRDYVYSVAFDPQGETLASGSFDRTAKLWEARSGKLL
                     RTLKGHEDWVRSVTFDPEGSTLASGSSDKTARLWDARSGKLLSTLQGNQDVVFTVVFD
                     PRGGMLAGGNRDFSIYLWEARSGKLLHTLEGHQGVVSSVAFDPQGGILASGSGDNTVK
                     LWEVDSGDLLRTLEGHTDRVLGVAFSADGLLLASVSLDRTIRLWSCETWEAVAVIGQL
                     AAPDGDWEPGLAFHPTLPLLATAGLEQDSTSHGRSRRIQLWKLDHKVLLRKRGRAAAT
                     RAVHHTTGKIVLVGDHSVGKSALGYRLIHGRFEKQESTHGQQFWVFPALGKRRADGTD
                     CEAILWDFAGQPDYRLVHALFVDNADLALVLFDASDLRDPLHGVGFWLKQLQTAQSTC
                     PIILVAAQSDRGACSLTPEELEAFCRKHGIVGPITTSAASGLGIEELVERMKVLIPWE
                     EKPATVTTTTFKRIKDYILGLKEEKKDKQTIVTPGELRGLLEATDTGWKFTDAEMLTA
                     VSHLENYGYLKRLRTSKGETRILLQPERLNNLASSLVLEARRNPKGLGALEEKRLLAG
                     GYDFPELRDLQVSERDVLLDSAALLFLEHNVCFREIDPLRVEPYLVFPELINLKKPVE
                     EDVATEDGVAYTVSGPTENVFASLVVLLGYTHTFTRTAQWHNNARYEVGDGLACGFRQ
                     ESERDGELDFVLSFGPTVGAPVRTLFQGLFESFLARRNLTVRRYEPVRCTRDHLLNRA
                     VVREAMQIGDESTFCSKCGEQVILPAMTEPIQLTQAVQAKVDLQRQAAEQRTRFEQAV
                     FRVRAFTVEQKITPPECFISYAWGVPEHERWVEKRLVTDLQKAGLQIVFDRKDNEFGD
                     SLTRFISRIPYCASVVVVGTPLYFQKFENRMSSTGSVVAAEVDLISDRLVGTQEEKRT
                     VKTLLLAGEKKTSLPPLMWDRIHADFRDESVYFTTSFDLILSLYQIPHTHPAVADLRE
                     SLRGPDTRFR"
BASE COUNT         2387 a         4076 c         4062 g         2528 t
ORIGIN      
        1 atcctgcaga gagctctgcg ttccttcgat ctgactcgaa aggaccgcct ccttgcgcac
       61 gtacatgaga acaagaaggt cagggttggg cagcgtctga atcgaaccat cgaggcgtcc
      121 aagcgcgaga tcggctttcg acaacaacgc ctggagctcg ccttcgacgc gaaccattgg
      181 cacgggtgga agcggcgccg ggatgaacgc acgatatcca gtcaattgct tctcgtattg
      241 ccccgcccgc cgtcggaaga cgacggcggc gttctttcgg ctgaccatgt ctgcctttag
      301 tatgaatcga gagcgacttt atagtaaagg cagcctttaa tatggcgacg aactccatcc
      361 atgttaaagg tagcctttca tacccctgcc gacccaaaac tctacccaca gtagccaaac
      421 atcgcacttc gccaacaccg aggtagcatc gggatgaagc gaggttgcga tgctcatcaa
      481 agaggctacg gctactccga ttgactacct gcgaacggct gtcgggcgcg acgatgcgac
      541 gtttcgggac gagcagcttg agtgcattga ggcggcggtg cggggggagc ggttgctggt
      601 tgtgcagcgg accgggtggg ggaagagcat cgtttacttc attgccacgc ggatgctgcg
      661 cgatcgcggc gccggggcca cgttaatcgt ttcgccgctg ctttcgttga tgcgaaacca
      721 gttggcggcg gcggcgcgga tcgggttgcg gtcggcgacg atcaactcgg cgaacaccga
      781 ggattgggac gctgtgattc gagccatgcg gaggaatgag atcgacgtcc tgctgatctc
      841 gcccgaacgg ctggccaatg acgcattccg gcagaacgtc ctcgtgccga tcggtggcaa
      901 catcgggctc ttcgtcatcg atgaggcgca ctgcatctcc gattggggcc acgacttccg
      961 ccccgactac cggcgcatct cgcaactgtt caagcttctt ccgccttcgc tgcccgttct
     1021 ggcgacgacg gcgacggcga acaatcgcgt cgtcgatgac gtgatggcgc aactcggcaa
     1081 tgtctcgatc tcgcgcgggc cgctggtgag gaaatcgctg cggctgcaga acatcctcct
     1141 ccccgatcag gcctcgcgtc tggcgtggct ggcgcagcat cttccaacgc ttcccggcag
     1201 cggcatcgtc tatacgctga cggtacgcga tgccgagcgc gtcgccgagt ggctgcggct
     1261 gcgcggcatc gatgcggttg cctatcacgc cgattccgcc gaccgcgaga tgctcgaagc
     1321 gagactcctc cgcaacgatc tcaaggtcct cgtcgccacc gtcgccctcg gcatgggttt
     1381 cgacaagccc gacctcggct tcgtggtcca ttttcagcgt ccgggatcgg tcgtccacta
     1441 ctaccagcag gtcgggcgcg ccggacgcgc cgtcgacgag gccttcggca ttctcctcgg
     1501 cggcgccgag gatgacgaga tcatcgactt cttcatacgg caggcgtttc cgccggaggg
     1561 cgacgtgctc gacgtcctcg aggcgctcaa ctgcgcggac gacggcctct ccgtgacgca
     1621 gatcgagtcg ttcgtcaatc tgccgttcgg gcgcatcgag cgcacgctca agctgctggc
     1681 caccgaaacg ccggcgccga ttcgcaagga aggatcgaag tggtatcgat cgccggtgcc
     1741 ttacgtcttc aatgctggga aggtgcagca gctcactttg atccggcgcc gcgagcagca
     1801 gcggatgcag gagtacatgc gctccaccga ttgcctgatg cagttcctgg ccgcggagct
     1861 cgacgatccg ttcgcggcgg cgtgcggtaa atgtgcgaat tgcgcgggcc gggtcgtgtc
     1921 agccgaggtg ccgatcgaag gagtgattgc cgcgaacgag ttcctgcagc gcacgcgcgt
     1981 gacgatcgag ccgcgcaaac tctggcctgt cgcggcactg gtcaaagacg gcttcaaggg
     2041 aaagatcgcg ccgtttcaga acgagcccgg acgcgcgctg tcgctttggg aagacggggg
     2101 atgggcggag ctggtgcgac gcggaaagta tgaagacgat cgtttcgacg attcgctggt
     2161 cgacgcctgc gcggcgctcg tcgccgactg gaagccggat ccacgcccgg agtggttgac
     2221 ctgcgtgccg tcgaagcggc atccgcaact cgttcccaat ttcgcgcagc gcctggccgc
     2281 gcgcctcggc atcccctacc gcgccgcgat cgtgaagagc gccgacaatc tgccgcagaa
     2341 gcagcgggag aactccgtac agcaggcgcg caacctcgac ggcgcgttcg cggtggatcg
     2401 agcagcgatg atgcccggcc cggtcctgct catcgacgac atgaccgatt ccggctggac
     2461 gctcacggtg accggcgcgc tgttgcgaag agcgcgctgc ccggccgtct ttccgctggt
     2521 cctcgcggat gcatcccgtt catgatggag gacgtgacgc aggaacagca ggtatcgcga
     2581 gacacgcagg ccgttctcct tctctgcagc cgtttcgcgc cgcgcgagca ggtcgagccg
     2641 ctcgacacgc gcgaattcag ccgcgtcgag gatttgctgc aacagcgttc gctgcgtccg
     2701 ggcgatctgc tcgatgacgc ggatctcgac tgggctgcga gtggcctcga cgctactcgc
     2761 atcgcgcagc tcctcgatcg cgcgatgggc ctcggcctcg ctaccgagcg atgggcgaac
     2821 ggcgggctct gggtgatctc gcgcagcgac gacgattacc ccgcgcgcct cctccagcac
     2881 ctcggccgta gcgcgccgcc gctgttgtgg ggcgtcggcg agcgaagcct gcccgctttc
     2941 ggcggcatcg ccatcgtcgg atcgcgcgag atcgatgatg ccgccgcgaa gtgggcggag
     3001 gatgtggcgg cggcgttcgc gcgcgaagaa atcaccgtca tctccggcgg cgctcgcgga
     3061 accgatcaga tcgcaaccgc cgctgcgttg aacgaaggtg gccgcgtcat cgccgttctt
     3121 ccggaaggac tcggccggcc gtccgtcgtg gggcattatc gcgaagcggt cgtcgacggc
     3181 cgcctcctgc tcctctcccc tgtttatccc gacgccggat tcaccgtcgg caacgcgatg
     3241 gggcgcaaca aagtcatcta cggaatcgcc gaggcggcgg tgatcgcgcg cgcggacgcg
     3301 aacagcggcg gaacgtgggc aggcgcggaa gcggaactga aacgcgagga cgccattcct
     3361 gtgttcgttc gctccgaagg tgaggttgcc gatggcaatc aggcgctgat cgccctcggc
     3421 gcgcgcgagt ttccggcgca gccgtggagc gatttgcgcg cgctgctcac gacgtcacgc
     3481 acgagtgcag aagcgcgtga gaagccggcg cccgggcagc gcagcttgtt tgaaaggtag
     3541 gggcccggat tcatcggcgg aaaacactta ccacagagac acagcgcggc atagccgcaa
     3601 ccaaagggaa cccaccaaat ctgcaaccaa tcagttttgg tgaaaatctt tgcgtcgcgc
     3661 gcagaaaccg ctggttagta gtacagaggt cacagagaag tcgatgtcag cacaagccat
     3721 tcaaggatgt gctgcgtggt gccgcccaca acctcccggt gtcgtgcatg gaagtgggag
     3781 caccgtgtaa tcgagcgtga agttggtcgt tgccgtatcg agatcggcgt tgccgctgta
     3841 gattgcggtg taggagtgcg tgcccgcgga tggttgaatc gtgatcgatg cgacgttccc
     3901 tactgtcgga gctgttccga cgtcgatgcc gccttcgcga aacgtcatcg tgccgccatt
     3961 gccgctgaaa gacgcaaacg tcgttgcctt cagcgtgagc ggctgacccg ccataattgg
     4021 cgatgccggt gtgccgctga gaccgacgag gggaatggcc ttcgtgacga cctgcgaaaa
     4081 cgtctcggtg tccgctgtgt ggttggcgtc gcccgagtat tcgccagcga gcgcatgcgt
     4141 cccgaccgtc gtgaacttgt acgtgagcgc cggcgggtga ttggcgtcgt aagccttcga
     4201 gagcaggacc gtgtctccgt cgcgaatcgt gagcgtgccg gacggctgca cgggaccggc
     4261 gtctgcttgt ttcgcgaaga tccttgcagt gatggtcgtt gtctgaccaa gttgcgaggg
     4321 actggggaac gcttcgatga cgaccacgga gtcgagcttc aatacggagc gcacgacact
     4381 cacgctcgcc ggcgggaaga tgtcgtctcc gacgaactcc gcggcgatcg tctgatggtc
     4441 tccgatgatg cgcggcacca gggtcatgag cgcggtgttc ccgttccaat tggcgaaccc
     4501 gttgaacacg ccgtctacgg tgatcgtgac gccgccgact ggcagaaatg tcgaggaagt
     4561 tgttgcggtc gtcgtgaggg ccaccggctc ctgaaagtgc aacgtcgaga gagatggcgt
     4621 cagcgtgagc ggcagcggtg tggcgccccc tgccgctgtt cgcgtgagaa gtgtgttgac
     4681 cgcgtcattc tgatcgtcga gcaggagaag gtcggccgag ccattgccat cgatgtcggc
     4741 ggcggcaaga tatgaccacg agcgcgaaac gacgcacggc acgcgcgcgc ggagcgtgcc
     4801 gtcgcctttg ccgaggtaca cccgattcga gatggcgaga tcgagcttgc cgtcgccgtc
     4861 gaaatcagcg gccacggcgt tggccggcga cgaagcgaga acgaggtcac tcccaagaag
     4921 aaatggctgc aggtccgtga aggtgccgtc accgttcccg agccgtatgc gcacggtatt
     4981 gttgtactgc tcctcgacca gatcggcctt gccgtcaccg ttgaagtcac cgacggcgat
     5041 ggctccaacg gtgttcgaaa cgtgacctcc cgcggaaaaa acgccgctgc cgttattcag
     5101 gtagatggtc atgccgttga tatcggtgct gacaaaatcg ggcctgccat cgccgttgaa
     5161 gtcggcgatc tggcctgggt tgcccgggag cgtcgtaggg agggcgaagg tgccatctcc
     5221 gttaccaaga aggagctgtc cgttaaagag aaagttgccg agcagaacgt cgagcttccc
     5281 atcgccgttc acgtccgcga gaaaaacgct ggtcaggctc gtaatcgctg tgggtgccgc
     5341 ggcagtgaac gttccatcgc cgttgctgag gtacgtccac aacttggatc cggcgacgac
     5401 gatgtcactc ttgccgtctc cgttgacgtc gcccgtggct aaggatgacg cggccgtgat
     5461 gccgggtgga agagcgagcg ccacaggcgg cccgagcccg tcaccgctgg tgagcatgac
     5521 gatgatctga tcgaccaggg tgacgacgtc cggcttgccg tcgccgttga agtccgcaac
     5581 ggcaaggcct ttcgggattg tgcgggaatt gtaaaagggg gccggagcag ggcgcacaaa
     5641 ggtcttccgg taggcgcgca gcgtaccgtc gccgttgccg tggagcagca ggagtccggg
     5701 agaagagaca gcgagaacgt ccggatgatg atcgccgtcg aagtccgcca cagcgagcgg
     5761 cccggagttg gcgacatacg cgcggacagc gaatgtgcca tcgccgttgc cgcgcatcac
     5821 cgtcaccacg tcgttcccac cggcgacgac gtcaagcttc ccgtcgccgt cgaagtcggc
     5881 ggcgacaccg ctgtagacac ccccgacaac gtacttcgcc ccatcggtga acgtgccgtt
     5941 gccattcccg agatgaacca gagctgtgcc gtcgaggcga gagatctcca cgatgtcgcg
     6001 cttcccgtca ccgttgaaat cgccaccggc cgtagtgcgc gcgaaggggt cgtgcgagat
     6061 tgtcgtggga ccgttgacgc tgccgtttcc gttgccggtg gcgagacgca gttcgtagaa
     6121 cgacgactcg atcacgtcgg cgcggccgtc tccgttgtag tcgccgctct cgatgttgtc
     6181 gatgctttgg atgaacggcc ccggctgcga gattccggcg gcaaacgtgc cgttgccaat
     6241 gccgcgaaac gtaaccagcg tttggttgtt ggcatccacg cgtgtgacgg cgatgagatc
     6301 gagcttgccg tcgccgtcga gatccgtgag cgcgagcggc gagaagccga tgatgtttgc
     6361 gatttgcgat gagatgagtg tgaacgagcc atctccgttg ccgtgatagg tctcgagtct
     6421 cgtgtcctgg ccgctcagga cgagatcgag cttgccgtcg ccgtcgagat cgccgagcgc
     6481 gatcctggcc ccgggggtcg tgatgggagt gctcacgggc gtacggaatg aaccgtcccc
     6541 attgccggcg agagtgagga gtacccagtt gttgctggga agaagcttct cgagcagcac
     6601 tgcatccggt ttcccgtcgt tgttgatatc acccgccttc atgtcgaaga cggcggcgga
     6661 gccataatcg atcgaggcgt tgcggaacag cgcctgtccg gcacccagcg gcgcgcagaa
     6721 cagcgacgaa gcgagaagca ggagtgctgg acggatcatc ggctgtcggg ctcggcgtct
     6781 ctcattatta tagacgtcgt ttcaccccta cagaaccacg ccaccgctcc tcgccagcca
     6841 tcgcgacggc tgaaacggag gaatggccag cggctcgatc aacggcatcc cctcgcgcca
     6901 gaacgaacgg aacggcagct tcttgcctgt ccggtagaag tgcatcagat aggccgaatc
     6961 acaagcggcg aagtcggaga cccattgatc gagatcgagg atgccggcgt cgtggaagag
     7021 ctgcaggctg taacgacaag cctgcttttc gtacgcggcc agctcgccga gaaactcttc
     7081 ggtcgccgcg gtgacggtcg tcgtgccgat ttcccgcgcg cgtggattcg tgttccattg
     7141 cacggtgtgc ccgaggagat gcgcgatgat gaaaagggca tcctcgctcg attggtcgta
     7201 gtcgaccgca atcgattccc catcgagatc gcccgtgaac ggatcggtca cgtcgctgat
     7261 cacgacctga acggagtagc gccgttcgat gtactcctcg atgatgttga aagcctcgcg
     7321 aaagcgcgat tcgtcagcgt agagcatgtg ggatggtaac gcggggtgtg gggtgtaggg
     7381 ggtggggtgt gggagatctc agggatggcc gctattggct gctcatcatc cttcacaact
     7441 ctcccacccc ccacacccta ccccccactc cccaacagct tcgcaatctg cacgtagtac
     7501 cccgccgcct tcccgcgatc gcctttgagc tgcgcggcgc gtgcggcgcc ggtcaggccg
     7561 ttgagccggt tcggcgaatc ggcaagtgag cgctcgtagg cttcgagcgc tttgtccggc
     7621 tggccgagct cgagcagcag atcgccgagc atctcgcgcg ccgggatgat cgagccgggc
     7681 gtgaccggac tcttgtccat cgagtcttcg agatcggcgg cggagcgcat cagcgtgaag
     7741 gcgccatcgt cgcgcttttc cgcatgggcg atccacgcat cagcaacacg gcgctgcact
     7801 tcgatctgat cggcccaata tccattcttc tgttcgatga gtttctggcg gagctgttcc
     7861 aagcgcgcca ccgcttcgtg cgcgtgcgcg acatcgccgc tgcgcgcggc acccactgcc
     7921 cgcgcgaaat gcgtgagcgc ctcggcatac gcataccgct cccacgcaaa cgccgcaggc
     7981 cgcacggtca gcgccgctgc ttctgaccaa ctcctgcgct ccaatgcgta gcgcgcaggc
     8041 acggctgctg ccgcgtagta gccggcgaag ttctcgacat tgagcgcttt gatcgtcgcg
     8101 atctggtcga cgagctcgcg gaccttttca tcctggccct gctgcaacca ggcgtaggcg
     8161 agatagtccc acgcgtgcag gctgtcgaag gacgcggtcc cgggatgcgt cttctccgcg
     8221 tgctcgcgcg ctgctttcgc cgaggcgaga ttcgagtcga tggtctcctt ccacatgccg
     8281 aggcggacgt agatgtgcga aggcatatga agcgcgtgcg gcgatgacgg cgcgatcttc
     8341 gcgtacgtgt tggccgcttt gagcgcgagc ggagccagcg ccggatagtc gaagctgtgg
     8401 atcagatagt gcgtgatgcc gggatgatcg ggatgcctgt cgagcaaccg gttgaagatt
     8461 gccgcggctc gcttctgata cgcgtaggtc ttgtcggtgg ggacggacat gccatgagcg
     8521 acgagcgcaa gaccgtagaa gatcgacgcc tcatcgtcat cgggaaaacg agcggtcaac
     8581 ttctccatct ccgcctcgta ggcacgcgca cgcgtgaccg tatcgagttt gtccgcgccg
     8641 cgataaaacg tcacgatggc gtcgatgtaa gcctgctcgc gcggcgtttg cgcgcccgca
     8701 gccttcgcct tctccgctgc tgcaacgccc cgcgccagct cctcgggtga atacggcgac
     8761 ggccacaccg ggtgatagtt gctcatcgcc acgccccacc aggcgatgcc gcatcgcgga
     8821 tccttcaccg caatgacgcg gaatgccttc tccgcttctt cgtaccagaa cgagtgcagc
     8881 agcgcgaccg cacgcgtgaa cgccgcctgc gatcccgcgc acgagatcgg gaaatcaacg
     8941 gtaccgaggc gctcgtcggc gggaccatcg tgatgatgct ccatctcctg ggcagccaac
     9001 ggcgcggcag taatcaagaa cgccgccgca acaaccgctc gccatttcct cgggaagaac
     9061 atgccggcct ccttcacctg atgagtcgca attcgatcat agctccgctt gcaatggacc
     9121 gaatccgttg tgtaccgttg agcgcggaac gagtctgatc gatgcgtacc cgcgctcttc
     9181 tcgccgccgc aatcctggcc tcacttctcc cggcgtcgag cgcgttcgcc ttctgcttcg
     9241 gaacgattca cgtccacgtc ttcaatgaca cgaatcggaa cggcgtcctc gatgccggcg
     9301 aagccgcggt gccaggcgtc gccgtccaac tcgaccagct tggcgatggc accgtcgaag
     9361 aaacgctgac aacgaacgcc aatggcgacg tcgatttcct cgcgcccgcc gtcgtctcct
     9421 accaggttcg catcgtcgcg ccggcgggca tggtccagac gtccgtcact ccgccgacct
     9481 tccccatcac ctgcgatggc gtcacgaacg tttcattcgg gctgatccag gcgattccgg
     9541 cggtgtcgcc aatgttgctg gcgctgctgg caatctcgct gatggctgtc gccgttgtaa
     9601 cgctgcgtcg ctgaaccctc ttatctgaac ctggtatcgg gtccgcgcaa tgactcgcgc
     9661 aaatcggcga cggctggatg agtgtgtggg atctggtaga gactcaggat gaggtcgaaa
     9721 gaggtcgtga agtatacgga ctcatcccga aaatccgcat gaatgcgatc ccacataagc
     9781 ggaggcagag aggtcttctt ttctccggcc aagagtagag tcttgactgt gcgcttttct
     9841 tcctgcgtgc cgaccagtct gtcgctgatc agatcgactt ccgcggcaac cacgctgccc
     9901 gtgctggaca tacgattctc aaatttttga aagtacaacg gagtgccaac cactacgacg
     9961 ctcgcgcagt acggaatccg gctgatgaag cgcgtcagac tgtcgccgaa ctcgttgtct
    10021 ttgcgatcaa agacgatctg aagccccgcc ttttgcaagt ctgttactag ccgtttctcg
    10081 acccagcgct cgtgctccgg cacaccccag gcgtaactga tgaagcactc aggcggcgtg
    10141 atcttctgct cgaccgtgaa tgcgcgtaca cggaacacag cctgctcgaa gcgcgtccgc
    10201 tgctcggccg cctggcgctg caagtccacc ttggcctgca ctgcttgcgt caactggatg
    10261 ggctctgtca tcgccggcag aatcacttgc tccccacatt tcgaacagaa agtgctctcg
    10321 tctccgattt gcattgcctc gcgaacgact gcgcgattca gcagatgatc tctggtacag
    10381 cgcaccggct cgtagcgccg cacagtcaga ttgcgcctcg caagaaagct ctcgaacaat
    10441 ccctgaaaga gcgtacgcac cggcgcgcca acggttggtc caaagctgag cacgaagtcc
    10501 agctcaccgt cgcgctccga ctcctgccgg aatccgcacg ccaaaccatc gcccacctcg
    10561 taccgggcgt tgttatgcca ttgcgccgtg cgagtaaatg tgtgcgtgta gcccagcaac
    10621 accaccagcg aagcaaacac gttctccgtc gggccgctca ccgtgtacgc cacaccatcc
    10681 tcggtggcta cgtcctcctc aacgggtttc ttgagattga tcagctcggg aaagaccaga
    10741 tacggctcca cgcgaagcgg gtcaatctca cgaaagcaga cgttgtgctc aaggaaaagg
    10801 agcgccgcgg agtcgaggag cacgtcgcgc tcgctcacct gcaggtctct caactcggga
    10861 aaatcgtagc cgccggcgag cagccgtttc tcttccagcg caccgagccc cttcggattg
    10921 cggcgcgctt ccaacacaag cgacgacgcc aggttgttga gccgttctgg ctgaagcagg
    10981 atgcgcgtct cgccttttga ggttcgcagg cgtttcaaat agccgtagtt ttcgaggtgg
    11041 cttacggcgg tcagcatctc ggcgtcggtg aacttccatc cagtatcggt ggcctccaga
    11101 aggcctcgca actcaccggg cgtgactatg gtctgcttgt ctttcttctc ttccttgagg
    11161 ccgaggatgt agtccttgat tctcttgaaa gtcgtggtgg tgaccgtcgc tggcttctct
    11221 tcccaaggga tgagcacctt catgcgttcg accagttcct cgatacccaa acccgaggcc
    11281 gcgctcgtcg tgatcgggcc aacgatgccg tgcttcctgc aaaacgcttc cagctcctcc
    11341 ggcgtcaacg aacaggctcc gcgatccgat tgtgcagcca cgaggatgat ggggcaggtg
    11401 ctttgcgccg tctggagttg cttcagccag aagccgacgc cgtgcagggg gtcgcggaga
    11461 tccgaggcgt cgaatagcac cagcgccaaa tcggcgttgt ccacgaagag cgcatggacc
    11521 agccggtagt cgggctggcc cgcgaagtcc cagaggatgg cctcgcaatc agtcccgtcc
    11581 gcgcggcgct tgccgagggc gggaaacacc cagaactgct gcccgtgcgt tgactcctgt
    11641 ttctcgaagc ggccgtggat caggcgatag cctagcgctg acttgcccac actgtgatcg
    11701 cccaccagca caatcttgcc cgtcgtgtga tgcaccgcac gcgtggcggc tgctcggccc
    11761 cgcttgcgca gcagcacctt gtggtcaagc ttccatagtt ggatccgccg ggatcttccg
    11821 tggctcgtcg agtcctgctc caagccggcc gtggctagca gcggcaacgt aggatgaaaa
    11881 gcgaggccag gctcccagtc accatcgggt gctgccagct gcccgatgac cgcaacagcc
    11941 tcccacgttt cgcagctcca gagacggatt gtgcggtcca ggctcacaga ggccagcaga
    12001 agtccgtcag ctgagaatgc gacgccaagg acacgatcgg tgtgcccttc gagcgttcgg
    12061 agaaggtcgc cgctatccac ctcccagagt tttacagtgt tgtcaccact tccgctcgcc
    12121 aatatcccgc cttgcggatc gaacgccacg ctagagacca ccccctgatg gccttcgagt
    12181 gtgtggagca gcttgccgct tcgcgcctcc catagatata tggagaaatc cctgttcccg
    12241 cctgctagca tccctccacg cggatcaaaa accacagtga agaccacgtc ttgattgcct
    12301 tgtagagtgc ttagcagctt gccgcttcgc gcgtcccaga gccttgctgt cttgtccgaa
    12361 ctcccactgg ccagcgtcga gccctcggga tcaaaagtca cacttctgac ccagtcctcg
    12421 tgtcctttga gcgtgcggag caacttgccg cttcgcgcct cccagagctt cgccgttctg
    12481 tcaaagctcc cgctggccag cgtctcgccc tgcggatcaa acgccacgct atagacatag
    12541 tcccggtgtc ctgcgagcgt gcggagcaac ttgccgcttc gcacctccca aagcttgacc
    12601 gttttgtcag cactcccgct ggccagcgtc tcaccctgcg gatcaaacgc cacgctcaag
    12661 agcgcagact cgtgcccttc gagagcccga agccgtttca cgcccggcgg cagcttcgtc
    12721 cgtaactcct cgctctgctg tggctcagcc cgtttcttct tcgccatacg gttctagctg
    12781 tttcagtctg ccgacgtggc agattggagt tattcgacac cgtcaccgaa ctcgtcgcaa
    12841 ccgaagatgc cacactccgg cctctacgca aagacgcttc gcgttcttta tttagcttga
    12901 ccacaccggg aagtcttccg ctcgaagtcc ccaagcgata tcgtgatggc cgaggtgtgt
    12961 cgatgaagaa gatgttgtgg ctgatctttc tgcttgctcc gtctgcgttt gcgcaattgc
    13021 ggccggtgaa cacgttttcg atcgtggcgc gcg
//
LOCUS       DATLVW010000542         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_434410, whole genome shotgun sequence.
ACCESSION   DATLVW010000542 DATLVW010000000
VERSION     DATLVW010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434410"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..730
                     /locus_tag="VNN25_16165"
     CDS             200..730
                     /locus_tag="VNN25_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93118.1"
                     /translation="MKLRILLTLATTVLFAGSLFAQPGSIEHSPAGCVLEGEMAIMSV
                     ETSDNGLLRAYFRRQGSTDWCYVDGQNRGKASTVTLPKFDPNEQIEYYFVVIDGKRVV
                     AKSPKIYTARSEHQCDAQFARHAIMITLECLPPGTNPISSAMNAGYAVNTVVGKDPAP
                     VQSPERPGQAPKPDQQ"
     gene            complement(782..1735)
                     /locus_tag="VNN25_16170"
     CDS             complement(782..1735)
                     /locus_tag="VNN25_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXH93119.1"
                     /translation="MPGVLVFCEVQDGKLKKASREALSIGRKLAEAAGGDLTAFAPDK
                     SAADDAGRYGAKKVFVADGGGYNTETYTAAMQAAIAQASPSIVLFGGTSNGRDLAPRL
                     AARLDAGVASDVDRLEWAGGKLKARRPVYSGKAFATVEVSSVPAIATTRINAFPAEES
                     GAGAAEVVEVSAPADAKAKVVETKVTESGELSIVEADIIVSGGRGLKEGANFSLIRDL
                     AHAIGGAVGASRATVDAGWIDHQHQVGQTGRVVSPNLYIAAGVSGAIQHLAGMSSSKH
                     IVAINKDPEAPIFRVADFGVVGDLFTILPALTEEVKKAKAH"
     gene            complement(1825..>2598)
                     /locus_tag="VNN25_16175"
     CDS             complement(1825..>2598)
                     /locus_tag="VNN25_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXH93120.1"
                     /translation="SVVCIAQVPDTETRIKIGGDGRRIDEAGVKYIVSPYDEYALEEA
                     IRLKEKEGGDVTVLGFGPDRVQQALRESLARGATKALHVKGESGDADSLGIAKVLAAA
                     IKTIPHDLVFFGKQGVGTDNSLVGPMVAELLGYPQINVVTHMETAAGKVTAHREIEGA
                     EEVIEAPTPAVITAQKGLNEPRYASLKGIMAAKKVTIDTKSIADLGLADSDIVNQRVT
                     IVSLELPPEKSGGRKIDGGDPAAAAKEIVRYMKEEAKAL"
BASE COUNT          465 a          866 c          761 g          506 t
ORIGIN      
        1 ctgaaagccg gtgaagagcc tcacatgttc tacgaggctg tgaaccgtta ccagctgggc
       61 gactacgatc tggcccgtca gctcatgacg cgggccatgc cggcgctccc tcagatcccg
      121 gccatcctcg cctaccgcga ccggattctg gcccagcatt gaaattttga cgtatcatgt
      181 cctcttgtca gaggtcgcca tgaaactccg tatcctcctc acgctggcca caacggtgtt
      241 gttcgccggg tccctcttcg cgcagcctgg cagcatcgag cactcacccg ccggatgtgt
      301 gctggaaggc gaaatggcca tcatgagcgt cgagacctcg gacaacggtc tgctccgcgc
      361 ctacttccgc cgccagggat cgaccgactg gtgctacgtc gacgggcaga accgcggcaa
      421 ggcctccacc gtgactcttc cgaagttcga tcccaacgag cagatcgagt attacttcgt
      481 cgtcatcgat ggaaagcgcg tcgtcgccaa gagcccgaaa atctacaccg cccgcagcga
      541 acatcaatgc gacgcgcagt tcgcacgtca cgcaatcatg attacgctgg agtgcctccc
      601 gccgggcacc aacccgatct cgagcgccat gaacgccggc tacgcggtca acaccgtggt
      661 cggtaaggat cccgcgcctg tgcagtcacc ggagcggccg ggccaggcgc ccaaacccga
      721 tcagcagtaa gagaagcagc caccaacttt tgaaaggccg ccgcagggcg gcctttcacg
      781 ttcagtgcgc tttcgctttc ttcacttcct cggtcagcgc cggaaggatc gtgaagagat
      841 cgccgacaac gccgaaatca gcgacgcgga agatcggcgc ttcaggatct ttgttgatgg
      901 cgacgatgtg cttcgacgac gacattcccg cgagatgctg gatcgcgcct gaaacgccgg
      961 cggcgatgta gagattcgga ctgacgacgc gtcccgtctg cccgacctga tgctgatgat
     1021 cgatccatcc ggcatcgacc gtcgcccgcg acgcgccaac cgcgccgccg atggcgtggg
     1081 cgagatcgcg aatcagagaa aagttcgcgc cctctttgag accacgccca ccagagacga
     1141 tgatgtccgc ttcgacgatc gacagctcgc ctgactccgt gacctttgtt tcgacgacct
     1201 tcgccttcgc atcggccgga gcgctcacct caaccacttc tgccgcacct gctcccgact
     1261 cttcggcggg aaaggcgttg atgcgggtcg tggcgatggc cggcacgctg ctgacttcga
     1321 ccgtggcaaa tgctttcccc gaatagacgg gacgccgtgc tttcagcttg ccgccggccc
     1381 attccaaccg atcgacgtcg gacgcgacgc ccgcatcgag gcgcgcggcc agacgcggcg
     1441 cgagatcgcg gccattggac gtgccgccaa aaagcacgat cgacggcgag gcctgcgcaa
     1501 tcgcggcctg catcgccgcg gtatacgtct ccgtgttgta accgccgcca tcggcgacaa
     1561 agactttctt cgcgccgtag cggccggcat catccgctgc tgatttgtcg ggagcgaatg
     1621 cggtgagatc accacccgcc gcctccgcga gcttgcggcc gatcgacagc gcctcgcgtg
     1681 aggccttctt cagcttccca tcctggactt cgcaaaaaac gagaacaccg ggcatagtgt
     1741 gacggggtct cgcggtcctc gcggtctcgt ggtctcgcaa ggcgctgact tcgcgaccat
     1801 tcgagtcctc ggacctcgca accgctacaa cgccttcgcc tcctctttca tatagcgaac
     1861 gatttctttt gccgctgctg ccggatcgcc gccgtcgatc ttccggcctc cgcttttttc
     1921 gggcggaagc tcgagggaaa cgatggtcac gcgctggttg acgatgtcgg agtcagcgag
     1981 gccgaggtcg gcgatcgact tcgtatcgat agtgaccttc ttcgcggcca tgattccctt
     2041 caacgatgcg tagcgcggct cgttcaatcc cttctgcgcg gtgatcacgg ctggcgtggg
     2101 cgcttcgatg acttcctcag ctccttcgat ctcgcggtgc gcggtcacct ttcccgcggc
     2161 tgtctccatg tgcgtcacga cgttgatctg cggatagcca aggagctcgg ccaccatcgg
     2221 accgaccaga gagttgtccg tgccgacgcc ctgcttaccg aagaagacga gatcgtgggg
     2281 aatcgtcttg atcgcagccg cgaggacctt ggcgatgccg agggagtccg catcgcccga
     2341 ttcacccttc acgtgcagcg ctttggtagc accgcgggcg agcgattcgc gcagcgcctg
     2401 ctgcacgcgg tcagggccga agcccagaac ggtgacgtcg ccgccttctt tctctttcag
     2461 acggatcgcc tcttcgagcg cgtactcgtc gtacggcgag acgatgtatt tgactccggc
     2521 ttcgtcgatg cggcgtccgt cgccgccgat cttgatacgc gtctcggtgt cgggcacctg
     2581 cgcgatacac actacgga
//
LOCUS       DATLVW010000543         5646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_477032, whole genome shotgun sequence.
ACCESSION   DATLVW010000543 DATLVW010000000
VERSION     DATLVW010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477032"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNN25_16180"
     CDS             <1..219
                     /locus_tag="VNN25_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HXH93121.1"
                     /translation="PVYNEIVVRTPFLAAQILADLEHEKILGGIPLGPFYEGHDNDFL
                     VAVTELHTREHLDHFVAALTAAISRERR"
     gene            216..1712
                     /gene="gcvPB"
                     /locus_tag="VNN25_16185"
     CDS             216..1712
                     /gene="gcvPB"
                     /locus_tag="VNN25_16185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXH93122.1"
                     /translation="MTTILESDAATTAVPQSTNREGLIFEHSKRGRRGYRLPDLDVPK
                     DAEALPASLRRGEIIDEVEVSEVDVIRHFTRLSKLNVSIDAGLYPLGSCTMKHNPRLN
                     EELARTPGFALSHPLQPDHQVQGNLALIWHIEQTLKEIFGLPRVTMQPVAGAHGELTG
                     ILMVHKALAKSGNARKYILIPDSAHGTNPASAAFAGYQIKELKSNSRGTVDIDTLAAA
                     VTEDVAALMITVPNTLGVFETDIKRFAEILHDKGAYLYCDGANLNSFVGIARPGDMGI
                     DVIHSNLHKTFSTPHGGGGPGAGPVGVSERLVPFLPTPTVEKREDGTFYLDHDHPDSI
                     GRVRAFNGNFNVLVRALTYMRSMGPDGLRRIAQIAVLNANYIRARLRGSYHLPYDAPS
                     LHEVVFSDKLQTPKDVHTLDIAKRLMDYGFHPPTIYFPLIVAGALMIEPTESEPKEEL
                     DAFCDAMIAIARECEERPELVRSAPHTTPVRRLDEARAARQPVLRWKR"
     gene            complement(1727..2686)
                     /locus_tag="VNN25_16190"
     CDS             complement(1727..2686)
                     /locus_tag="VNN25_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93123.1"
                     /translation="MTLIALSAIAAPLIVLVVARADLYHFFRDSFVIIPRAIDAIWSL
                     PAKPLPSLALLVHPVQLWDWLASEAARYYLPPVFFGFLLALAVRKRDMRIAIVAIFSI
                     ILFRTAAGRCSWSHTRFGIPLLGIAVVAFLLEPLCRDWLRRRRSLWRGIAIVVFAILG
                     FRYFEIALNATLGWKFIAGWPARQRHEGMVPYPMTRGRGIYTWAENAADLAALRDLAQ
                     QAGPGPIFDLSGERALYYLLDRRPATRCPDIAMLSTPELSAEALHQLAAHPPVFVVLE
                     GMKVFGSLDGIANRDRIPPIAAWIDTNYPVRVKAGRYLVGFRN"
     gene            2827..3138
                     /locus_tag="VNN25_16195"
     CDS             2827..3138
                     /locus_tag="VNN25_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93124.1"
                     /translation="MILLRPPTPDSRRPQYASNAHTAINSGSHRKYGKTGTSPAPIAQ
                     SARITAAMSPPVDAVTSAAIGTSIASAAASSTVSSRRVFFGDGLLPRTNASRPPSVAK
                     P"
     gene            3256..3399
                     /locus_tag="VNN25_16200"
     CDS             3256..3399
                     /locus_tag="VNN25_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93125.1"
                     /translation="MTTKTAIVVMMVAVRFEGVVGRESGVSRALSASALRALAIVNAM
                     TTK"
     gene            complement(3598..5172)
                     /locus_tag="VNN25_16205"
     CDS             complement(3598..5172)
                     /locus_tag="VNN25_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXH93126.1"
                     /translation="MSVIGRLFATKSLDDLIAGTQEEGHQLKKSLGPWNLIALGIGAI
                     IGAGIFATIGTAAAGDAHRPGAGPALMISFVLTAVVCGFTALCYAEFASMVPVSGSAY
                     TYSYATLGELVAWIIGWDLMIEYAIGNVAVAISWANYMNTLLEGFGIVLPGWLTVDYG
                     TAMSKMPQVVAAAPHIFGVPIIFNILAVGIVTLITIVLVWGIREAASFNFWMVAIKLG
                     VLAFFVFVSFKYVKPAHWHPFAPNGFHGIATGAAIVFFAYIGFDAVSTTAEEAKNPKR
                     DMPIGIIGSLIICTIIYAIIAAVFTGMIPYADLVKKLSSEQAEPLTLAMKYATMPNWM
                     VGVVAFGSVVAHTAVLLVFQLGQPRIFFAMSRDGLLPPFFARVHPKFRTPHVATIITG
                     VFVGVAALFSSLDAMVDLTNIGTLFAFFLVCIGIIILRVKDPDRERSFKVPGGTWFLP
                     VLGALSCLFLAKYLPKESWWRFGIWLLVGMVVYLSYGYRNSKLRHRGNGPLLPPGGMV
                     THDFNPEQQLPEKDVE"
     gene            5237..>5646
                     /locus_tag="VNN25_16210"
     CDS             5237..>5646
                     /locus_tag="VNN25_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93127.1"
                     /translation="MRIAAALLALAALTCATQSKGPACIDLSGTYKLAEQPVRKGTGS
                     AAFVFGEGASLARVETLTISQPGCRIELQAKGDGGKTYDAVLENDLDWTDDGVSATWS
                     PEKTTSSILAGASSRTRTLTMRLSPQRDMLTISS"
BASE COUNT         1176 a         1752 c         1808 g          910 t
ORIGIN      
        1 ccggtctaca acgagatcgt cgtgcggacg ccgtttctgg ccgcccagat tctcgctgac
       61 ctcgagcacg aaaagattct tggcgggatt ccgctcggcc cgttctacga aggtcacgac
      121 aacgacttcc tcgtcgccgt cacggagttg cacacacgcg aacatctcga tcactttgtc
      181 gccgcattga cggcagcgat ctcccgggag cggcgatgac cacgatcctc gaaagcgatg
      241 ccgccaccac tgcggtcccg cagagcacga atcgcgaggg actgatcttc gagcattcga
      301 agcgcgggcg gcgcggctac cggctgccgg atctcgatgt tcctaaggat gccgaggcgt
      361 tgccggcgtc gcttcggcgc ggcgagatca ttgatgaagt cgaggtatcc gaggtcgatg
      421 tgatccgtca cttcacgcgg ctttcaaagc tgaatgtgtc gatcgatgcc ggcctttacc
      481 cgctgggatc gtgcacgatg aagcacaacc cgcggctcaa cgaggagctg gcgcgcacgc
      541 ccggcttcgc gctctcgcat ccgctgcagc ccgatcatca ggtgcagggc aacctcgcct
      601 tgatctggca catcgagcag acgctgaaag agatcttcgg tctgccgcgc gtaacgatgc
      661 agcctgtcgc cggcgctcat ggcgagctga cgggcatcct gatggtgcac aaagcgcttg
      721 cgaagagtgg caacgcacgc aagtacatcc tcatccccga ttccgcgcac ggcaccaacc
      781 cggcctcggc agccttcgcg ggctaccaga tcaaagagct gaagtcgaac agccgcggca
      841 ccgtcgacat cgacacgctc gccgccgcag tgaccgaaga cgtcgccgcg ctgatgatca
      901 ccgtgccgaa tacgctcggc gttttcgaga ccgatatcaa acgtttcgcc gagatcctgc
      961 acgacaaggg cgcgtatctc tactgcgacg gcgctaacct caactcattc gtcggcatcg
     1021 cccgccccgg cgacatgggc atcgacgtca tccactcgaa tctgcacaag acgttttcaa
     1081 cgccacacgg cggcggcggg ccgggagcag gaccggtcgg cgtttccgag cggctcgtgc
     1141 cgttcctgcc gactccgacc gttgagaagc gcgaagacgg aacgttctat ctcgatcacg
     1201 atcatcccga ctcgatcggc cgcgtccgcg ccttcaatgg caatttcaac gtgctcgtcc
     1261 gcgcgctgac gtacatgcgc tcgatgggtc ccgacggcct ccgccgcatc gcccagatcg
     1321 ccgtcctgaa cgcgaactac atccgcgccc gtctgcgcgg ctcgtatcac ctcccctacg
     1381 acgcgccgtc gctgcacgaa gtcgtcttca gcgacaagct ccagacgccc aaagatgtcc
     1441 acacgctcga catcgccaag cgcctgatgg actacggctt ccacccgcca acgatctact
     1501 tccccctgat cgtcgccggc gcattgatga tcgagccgac cgaaagcgag ccgaaagaag
     1561 agctggacgc tttctgcgac gcgatgatcg ccatcgcgcg cgaatgcgaa gagcgtcccg
     1621 agctggttcg cagcgccccg cacacgacgc cggtgcggag gctggacgaa gcgagagcgg
     1681 cgcggcagcc ggtgttgcgg tggaaacgct gagacgacat ccaccgtcag tttctgaagc
     1741 ccactaagta ccgtcctgcc ttcactcgca ctggatagtt tgtgtcgatc catgccgcga
     1801 tcggcgggat gcggtcgcgg ttggcgatgc cgtcgagtga gccgaagact ttcattcctt
     1861 cgaggacgac gaagactggt gggtgcgcgg caagttggtg tagtgcttcg gcgctcaact
     1921 ccggagtgga gagcattgcg atgtcggggc agcgggtggc ggggcggcgg tcgagcaggt
     1981 agtagagagc gcgttcgccg gagaggtcga agatcggacc gggaccagct tgttgggcga
     2041 ggtcccgcag cgcggcgagg tcggctgcat tttcagccca cgtgtagatt ccgcggccgc
     2101 gcgtcatcgg atacggaacc atcccttcgt gacgctgccg ggcgggccag ccggcgatga
     2161 acttccatcc aagcgtggcg ttcagcgcga tctcgaaata acggaagcca aggatcgcga
     2221 acacgacgat cgcaatgcct cgccacaggc tccgccggcg tctcagccaa tcgcgacaaa
     2281 gcggctcgag cagaaatgcc acgacggcga tgccgagcag tggaatgccg aaacgcgtgt
     2341 gactccaact acaacggccg gcagcggtgc ggaagagaat gatcgagaag atggctacga
     2401 tggcgatgcg catgtcgcgc ttgcgcacgg caagggcgag aaggaagccg aagaagaccg
     2461 gcggcaggta gtagcgcgcg gcttcggatg ccagccaatc ccagagttgt acagggtgaa
     2521 cgagaagcgc cagcgacgga agtggtttcg ccggcagcga ccagatcgcg tcgatcgctc
     2581 gcggaatgat cacgaacgag tcgcggaaga agtggtacag atcggcgcgc gcgacgacca
     2641 ggacgatgag cggcgccgcg atcgccgaca gagcaatcaa cgtcactctt cgcgtgtaga
     2701 tcaaaatgac gatcgcgctc gccgcaaccg cgcagatacc gacgtcgagg ctccagagaa
     2761 tgccaagcga ggaggcgatg gcagcgacgt agaggagcgc gggagtcgaa gtcgtatcgg
     2821 accgcgatga tccttctccg acccccgact cccgactccc gacgcccgca gtacgccagc
     2881 aacgcccaca ccgcaatcaa cagcggcagc catcgaaaat acggaaagac cggcacctcg
     2941 cctgctccga tcgcgcagag tgccaggatc acggcagcga tgagtccacc ggtcgacgcc
     3001 gtcacttccg ccgcgatcgg caccagcatc gcaagcgcgg cagcgtcaag caccgtctcc
     3061 agccgccgcg ttttcttcgg cgatggtttg ctcccgagga ctaacgcgtc gaggccgcca
     3121 tcagtggcaa agccgtgaag gaaaaagatg tctccgtagg gtcgtccgcc atcgagcatc
     3181 accgacgcgg gcgtgaggtg ctctccttcg tggaacatct ccatgaatga gtacgggtga
     3241 tcgtgcacga ccagcatgac gacgaagacg gcaatcgtcg tgatgatggt tgcggtgcgt
     3301 ttcgagggag tcgtgggtcg ggagtcggga gtctcgcgtg cgctcagcgc cagcgcgctg
     3361 agagcactgg cgatcgtcaa cgcgatgacg acgaagtaat cgaacccttt tggatacttc
     3421 gccagagtca cggcaccgat gccgccagtt ggaaccgaaa accacccgat gcaatggggg
     3481 gcgatcgcga agacgagaag ggcgccaagg aaggcaccga gcagcggcga ttgaaaccaa
     3541 agtcgccgcc gggccggcgg cggtccagcc ggcgagccgc cggcgctact cttcgggcta
     3601 ctccacatcc ttctccggca actgctgttc ggggttgaag tcgtgcgtca ccatgccgcc
     3661 ggggggaagc agagggccgt tgccgcggtg gcgcaacttg ctgttgcgat agccgtatga
     3721 gaggtagacg accatcccga cgagcagcca gatgccgaag cgccaccacg actctttcgg
     3781 gaggtacttc gccaggaaga ggcacgacag cgcgccgagc acggggagga accacgtgcc
     3841 gcccggaacc ttgaacgacc gctcgcgatc ggggtccttg acccgaagaa tgatgatgcc
     3901 gatgcagacg aggaaaaacg cgaagagcgt tccgatgttc gtcaggtcaa ccatcgcgtc
     3961 gagcgaggag aagagcgcag ccacgccgac gaagacgccg gtgatgatcg tggcaacgtg
     4021 tggcgtgcgg aacttcggat ggactcgcgc gaagaatggc ggcagcaagc cgtcgcgcga
     4081 catcgcgaag aagatgcgcg gctgaccgag ctgaaacacg agcagaacgg cggtgtgagc
     4141 gacgaccgaa ccgaaggcga cgacgcccac catccagttc ggcatcgtgg cgtacttcat
     4201 cgccagcgtc agcggttcgg cttgctcgct ggagagtttc ttcaccaggt cggcgtacgg
     4261 gatcatgccg gtgaagacag cggcgatgat cgcgtagatg atcgtgcaga tgatgagcga
     4321 gccgatgatg ccgatcggca tgtcccgctt cggattcttc gcctcttcgg ctgtcgtcga
     4381 aacggcatca aacccgatgt aggcaaagaa cacgatcgcg gcaccggtgg cgatgccgtg
     4441 aaagccgttt ggtgcgaacg gatgccagtg cgccggcttc acgtacttaa agctgacaaa
     4501 gacaaaaaac gcgagcacgc cgagcttgat ggccaccatc cagaagttga agctggccgc
     4561 ctcgcgaata ccccacacca gcacgatcgt gatgagcgtg acgatgccga cggcgaggat
     4621 gttgaagatg atcggaacgc cgaagatgtg cggcgcagct gctacgacct gcggcatttt
     4681 cgacatcgcc gtgccgtaat cgacggtcag ccatccgggc aggacgatgc cgaagccctc
     4741 cagaagcgtg ttcatgtagt tcgcccagga gatggcgacg gccacgttgc cgatcgcgta
     4801 ctcgatcatc agatcccagc cgatgatcca cgccacgagc tcgccgagcg tcgcgtacga
     4861 gtacgtgtac gcggagcccg agacgggcac catcgaagcg aactcggcgt aacagagcgc
     4921 cgtgaatccg cagacgacag cggtgagaac gaacgagatc atcaatgcgg gaccggcacc
     4981 tggccggtgt gcgtcaccgg ccgcagcggt tccgatcgtg gcgaaaatgc ccgcgccgat
     5041 gatcgcgccg atgccgagtg cgatcaggtt ccagggaccg aggctcttct tgagttgatg
     5101 cccctcttcc tgggttccgg cgatgaggtc gtcgagcgat ttggtggcga aaaggcggcc
     5161 gatcacagac atgcgtgccg cgaatcatgg gcgcgcggcg gcggagggtc aagtgtcgcg
     5221 gcgtatgatg cccgcgatgc ggatcgctgc ggcactcctc gcgcttgcgg cgctcacgtg
     5281 cgccacgcag tcgaaaggac cggcgtgcat cgacctctcc ggaacctaca aacttgcaga
     5341 acaaccagtg cgcaaaggca ccggttcggc ggcgttcgtg ttcggcgaag gtgcttcgct
     5401 cgcaagggtt gaaacgctga ccatctcgca gccgggttgc cggatcgagc tccaagcgaa
     5461 gggcgacggc ggcaagactt acgacgccgt tctggagaac gatctcgact ggacggacga
     5521 cggagtctcg gcaacgtggt cgccggagaa gacgacctcc tccatcctcg cgggtgcatc
     5581 gagccgcacg cgtacgctga caatgcgcct ctcgccgcag cgcgacatgc tgacgatctc
     5641 atccga
//
LOCUS       DATLVW010000544         3342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_192578, whole genome shotgun sequence.
ACCESSION   DATLVW010000544 DATLVW010000000
VERSION     DATLVW010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192578"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VNN25_16215"
     CDS             <1..525
                     /locus_tag="VNN25_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93128.1"
                     /translation="YYTQFISEAALDAVVPQRPAMNGEIQPTYFEAALVAPLAPSAAA
                     APAATEVNIAQRANEIQSTLSAATQTRTTTAVAQVANPNGSTQLLVGSSERVLRPAQL
                     AALRDGETAVSGQGHAEATVISAAHANGQTVQAVAASRPICPSCAGLISQAGATAASA
                     LKQVIDVALRMVLP"
     gene            522..1133
                     /locus_tag="VNN25_16220"
     CDS             522..1133
                     /locus_tag="VNN25_16220"
                     /inference="COORDINATES: protein motif:HMM:NF016137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HXH93129.1"
                     /translation="MKLRYDAERLQIVLESLSPHHRIAFGATCCERLLPNYAAFAKDT
                     GWGDPSVMRDALAYIWMTIGKGTANDAEIARTIANCEALIPDTEEFSSAYTSAAVDAG
                     TAIVETLKALLDPTARHIVDVASFCRDTVHMFIQERDALDFGADSSFDAKIADDPLMR
                     SELGQQAAVLQELKAQPDLNVFFLKRLRTEAQASGFSNIGLPA"
     gene            1214..2827
                     /locus_tag="VNN25_16225"
     CDS             1214..2827
                     /locus_tag="VNN25_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HXH93130.1"
                     /translation="MLSTAVHALAQSTYDPPPALPRKTLTPIRAPLGGVQYPQALGFG
                     DNGESGTYTYDGSGNITAIGADTFLYDAEGRVVTAVIRGVTGTYTYDAFGNRKTGTGE
                     TNCYGQTVCATPLTLDSTTNRLLTTTNGTTTNVGYDAAGNITSASGGVYAYDGTGMMT
                     EATVGSDDRQFVYTADDERIAVRQGQSWMWTVRGLDNKVLREFTSTEPNNSAGLPTAG
                     GTWIKDYIWRDGLLLASTDSTGTYHYHLDHLGTPRLITNASGVKVAEHAYYPFGAEIS
                     LTPHEANEEAMKFTGHERDIVVGDGYTLDDMHARYYNATLGRFLSADPVLGGPDDPQS
                     WNRYSYARDNPLRFSDPTGRQVAQEIYAAGAFAGAYILCSYAASPSAAVPGKTNVQVL
                     VQTAIDGFSAVKLLLGLIPGRQRTNIPTTPPPPATTPSQSGTPGTQATTGVNPAAPIQ
                     ARVVNTSHGPVTIPSDYTPRVSDSGKGTVYQAPGATGNADMIRVMNPTARYPTGYVVI
                     HNSYGQPVTIDGKTGSPAETHHPIEEKLQ"
     gene            2824..3219
                     /locus_tag="VNN25_16230"
     CDS             2824..3219
                     /locus_tag="VNN25_16230"
                     /inference="COORDINATES: protein motif:HMM:NF039679.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HXH93131.1"
                     /translation="MITEKDDAFEVLDNDLVVSTCRRDPWSWRLELHSPDVTWTLTIG
                     GAFSIMQSTVDKSAATTEAVADLLGQSVVAMRARKADSELEVRFGDGWVLTVEPDADY
                     EAWEMYSTRGERLIAVPGDGVAKWGEIHR"
BASE COUNT          735 a         1003 c          973 g          631 t
ORIGIN      
        1 tattatacgc agtttattag cgaagcggca cttgacgccg tcgttcccca acgtccggcg
       61 atgaatggcg agatccagcc aacctacttc gaagccgcgt tagtagcgcc tctcgccccg
      121 agcgctgccg cggctccggc agcgacagaa gtcaatatag cacaacgcgc taacgagatt
      181 caaagtacac tgtcggctgc aacacagacg cggactacaa cggcggtggc ccaggtcgcg
      241 aatccgaatg gtagcacgca gctgttagtg ggctccagtg agcgagtact gcggccagca
      301 cagcttgccg cactgcggga tggcgaaacc gccgtatcgg gtcagggtca cgcggaggca
      361 accgtaatta gtgcagcgca tgccaacggc caaacggtgc aagcggttgc agcgagcagg
      421 cccatttgtc cgtcttgtgc tggtctgatt tcgcaagcag gcgcgacggc cgccagcgcg
      481 ctgaaacaag tgattgacgt cgccctgagg atggtccttc catgaaactc cgttacgatg
      541 ctgagcgtct ccagatcgtg cttgaaagct tatcgcccca ccatcgcatt gctttcggcg
      601 cgacctgttg cgaacgcctg cttcccaact atgccgcatt tgcgaaggac accggatggg
      661 gagatccgag tgtgatgcgc gatgcgctgg cgtacatttg gatgacaatt ggaaagggta
      721 cagccaacga cgctgaaatc gcgcgaacga ttgcaaattg tgaagcgctc attcccgaca
      781 ccgaggaatt ttcatcggca tatacctcgg cggcggttga cgcgggaact gcgatcgtgg
      841 aaacactaaa ggcgctattg gatccgacgg cacgtcatat tgttgatgtc gcgagcttct
      901 gtcgtgacac cgtgcacatg ttcattcagg aacgagatgc tcttgacttc ggtgctgact
      961 ccagcttcga cgcgaaaatc gccgacgatc cgctgatgag gagcgaactt ggccaacaag
     1021 cggccgttct ccaagaactg aaggcacagc cggacctcaa cgtgtttttc cttaagcgcc
     1081 ttcgaacgga agctcaagcc agcggcttca gcaacatcgg tctcccagcg taattgcgtc
     1141 gtcggtatct tgcacatgtt ctgcgcaaag tgaaatgatg cgacaccatg cattcgtcgt
     1201 attcttgatt gtgatgctga gcacggctgt tcacgcattg gcgcaatcga cgtacgaccc
     1261 accgcccgcc cttccgcgca aaacgctaac tccgatccgc gcgcctcttg gcggtgtgca
     1321 atacccgcag gcgctcggct ttggcgacaa tggcgagagc ggcacttaca cctacgacgg
     1381 ctcggggaac atcactgcaa taggcgcgga tacgttcctt tacgacgcgg aagggcgggt
     1441 ggtaacggcg gtcattcgcg gcgtcactgg gacctacacc tacgacgcgt tcgggaaccg
     1501 gaagacgggc acgggcgaga ccaactgcta cgggcagacg gtctgcgcca cgccgctgac
     1561 gctcgactcg accacgaacc gtttgctgac aaccaccaac ggcacaacga ccaacgtagg
     1621 atacgacgcc gccggaaaca ttacatcggc ctcgggcggc gtctacgcgt acgatggtac
     1681 cggcatgatg accgaggcca cggtggggag cgacgaccgg cagttcgtct acaccgcgga
     1741 cgacgagcgg atcgccgtgc ggcagggcca gtcatggatg tggaccgtgc gcggcctgga
     1801 caacaaggtg ctccgcgagt tcacaagcac cgagccgaat aactcggccg gtctccctac
     1861 ggccggcggc acgtggatca aagactacat ctggcgcgac ggactgctgc tggcctcaac
     1921 cgacagcacc ggcacctatc actaccacct cgaccacctc ggcaccccgc gcctgatcac
     1981 gaacgcgagc ggcgtgaaag tcgccgagca cgcctactac cccttcggcg ccgagatcag
     2041 ccttaccccg cacgaggcga acgaagaggc catgaagttc accgggcatg agcgcgacat
     2101 cgtggtcggg gatgggtaca cgctcgatga tatgcacgcc aggtattaca acgcgacctt
     2161 ggggcggttt ctgagcgcag atcctgtgtt gggtggtcca gacgaccctc aatcgtggaa
     2221 ccgctactca tacgcgcgcg acaatccgct tcgcttctcg gatcctaccg gccgtcaagt
     2281 cgctcaagag atatacgctg caggtgcctt cgctggcgcc tatattctct gctcctacgc
     2341 tgcatctccg agcgcagcgg tgcctggaaa aacgaatgtg caggttcttg ttcaaacagc
     2401 tattgatggc tttagtgctg tcaagcttct tctggggttg attccaggtc ggcaacgaac
     2461 gaacattcct acgactcctc ctccgccagc gacgacacct tctcagtccg gtacgccagg
     2521 aacccaagcg acgaccggtg tgaatccagc cgctcctatt caggcgcgag tggtgaacac
     2581 gagccacggc ccagttacca tcccatccga ctacacgcca cgagtgtccg acagcggcaa
     2641 ggggacggta tatcaggcgc ctggcgcgac cggcaatgca gacatgattc gcgtaatgaa
     2701 tcccacagca agatacccca ctgggtacgt cgtcattcat aactcgtacg gccagcccgt
     2761 gacaatagac ggcaaaaccg gcagccccgc ggagacgcat catcccattg aggagaagct
     2821 gcagtgatca ccgagaagga cgatgcattc gaagttcttg acaacgatct ggtggtgagt
     2881 acgtgtcggc gcgacccttg gtcgtggcga cttgaactcc attcacctga tgtgacctgg
     2941 acgctcacga tcggaggcgc gttttccatt atgcagagca cggtcgataa gtctgcggcc
     3001 acgacggaag cagtcgcaga ccttcttgga cagtcggttg tagcaatgcg cgctcgtaaa
     3061 gcagacagtg agctcgaagt cagattcggc gatggttggg ttctcaccgt cgaaccggat
     3121 gccgattacg aagcctggga aatgtattcg acgcgtggcg agaggcttat tgctgtccct
     3181 ggagatggcg tagcaaaatg gggagagatc catcggtaac agatccgccc atcactcgtg
     3241 tagcgctttc gaggtacatc cctctcagct cctcggacac gcgagacgag tccggacaat
     3301 ccagcggcgc gcctccgacg cggaaacggg gttctcctat gg
//
LOCUS       DATLVW010000545         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_192595, whole genome shotgun sequence.
ACCESSION   DATLVW010000545 DATLVW010000000
VERSION     DATLVW010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192595"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>2563
                     /locus_tag="VNN25_16235"
     CDS             30..>2563
                     /locus_tag="VNN25_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93132.1"
                     /translation="MAISGLGNGCNQLSGSFIVRDVAYDATGLLTRLAVDATQYCETS
                     ARALHVYLRYGSTVVPLIVPQTTAKPGADQQIAPGASATLDGSQSVAQDGGALIYSWS
                     QIYGPPVQLVGAATAKPSFVAPVPSAEWLVLVFQLTVTDSGGTTSTATVDVVVSSNHP
                     QSEIRIVSSAGDPVGQGVVLDETVSTSKLTLGRNAHNGIDLKYIFGGATPETLQFQAA
                     APNNAAVALGRYPLAYRYPFFAPSGKAGLDAALGLGCNQSVSRFIVEDLAYDASGNVT
                     RLALDFAQSCSDVPGAPPLFGYIRLNSVVPITSRAPTAWAGASVLASLGQSVALDASA
                     STGGAAAIIAYAWQQLSGPPVTLAGAGTSTVTFTAPSVPSAGATFIFQVTVTNIGGYS
                     DTDQVQVHVQGSAETKSFIALDPSGYDFVTGGQSALLDANLGTLAINQSVDVYGREHA
                     MLTFNDIDTWTVDVSSNQGQMLRPGIYPAVDTSANQAAVSLSGGGRGCDQTVGSLTID
                     DVERDASGAITSLALEFATRCDHGAPTAGIVRYVSTVPVPAPTALASAGPPQAATGGD
                     VVTLSAALSSGGPTGIVSYAWQQIAGPSVTLADSSGVNAAFVAPAAGGTLQFQVTLKT
                     ADGQTATSVVTVTVQPSGTARTVLVLESDPGTTMTFGQNLVLSDVLSPTITSISGNGL
                     NVHLADAYDTELYLWAPFGQPLAVGTVYEGGRMFNGENSHRLAGFPGLEFEFDSFYCT
                     AAWGRFVVRELTTDASGTVTALAVDFQQTCVPQNGYVRGWLRVNSTVPVGDGLPLASA
                     GPDLAVQAGRTIDVDGRASSAGPTSQATYQWRQLSGPPVQLTGTTTP"
BASE COUNT          453 a          827 c          808 g          475 t
ORIGIN      
        1 catcgttcca acagccgggg catcccggca tggctatcag cgggctcggc aatggctgca
       61 accagctgag cggatccttc attgtacggg acgttgccta tgatgctacg gggttactga
      121 cgcgcctcgc cgttgacgca actcagtatt gcgagaccag cgcccgcgca ctgcatgtgt
      181 acttgcgtta cgggagcacg gtggtgccat tgatcgtgcc gcagacgacc gcaaagcctg
      241 gcgcagacca gcagattgcc ccgggtgcga gcgcgacgct tgatggcagt cagtcagtcg
      301 cccaggatgg cggcgcgctc atctattcgt ggagccagat ttacggcccc cctgttcagc
      361 tggtcggcgc agcgaccgcc aagccatcat ttgtggcgcc ggtgccctct gcagaatggc
      421 tcgtactggt ctttcaactc acggtgacgg actccggggg tacgacgtcg acggcaaccg
      481 tggacgtcgt cgtgagctca aaccatccac aatccgagat caggatcgta agcagcgccg
      541 gcgatccagt cggtcagggc gtggtgctcg acgagacggt cagcacatcg aaattaaccc
      601 ttgggcgcaa tgcccacaac gggatcgacc tgaagtacat attcggcgga gcgacgcccg
      661 agacattgca gttccaagcg gcggcgccga acaacgcggc cgtcgcgctc ggtcgctatc
      721 ctctcgcata tcgttacccg tttttcgcgc cgtcaggcaa ggccggcctc gacgccgcgc
      781 tcggcctcgg ctgcaatcag tcagtcagtc gttttatcgt cgaggatcta gcctacgacg
      841 cctccgggaa cgtgacccgc ctcgctctcg acttcgctca gtcgtgttcc gatgtgccag
      901 gtgccccgcc gcttttcggc tacatacgcc tgaactccgt ggtaccgatc acgagccgcg
      961 caccaaccgc gtgggcgggt gccagcgtgc tggcctcgct cggacagtcg gtcgcgctcg
     1021 acgccagcgc ctctaccggc ggcgccgccg cgatcattgc gtatgcatgg caacagttga
     1081 gcggcccgcc ggtgacactg gccggcgcag gtaccagtac cgtaacattc accgcgccgt
     1141 cggtgccctc cgccggcgcg accttcattt ttcaggtgac cgtcacgaac atcggcgggt
     1201 actccgacac cgatcaggtt caggtgcatg tacaggggtc cgcggaaacc aagtcgttca
     1261 tcgcgctcga tccctccggc tacgatttcg ttaccggagg gcagagcgca ctcctcgatg
     1321 ctaaccttgg aacactagcc ataaatcaat cggtggacgt ttacggccgc gagcatgcaa
     1381 tgctcacgtt caatgacatc gatacctgga cggtggacgt gagctccaat caggggcaga
     1441 tgctgaggcc aggcatctac ccggcggtgg ataccagcgc caatcaggcg gcggtgtcac
     1501 tgtccggcgg tggccgcgga tgtgaccaga cggttggttc cttgacgatt gatgatgtcg
     1561 agcgcgatgc gagcggcgcg atcacctcgc tcgcgctaga gttcgcgacg cgctgcgacc
     1621 atggagcacc gactgccggt atcgttcggt acgtgagcac ggtgccggtt cctgcgccga
     1681 cggcgttggc gtcagcggga cctccccagg cggcaacggg cggcgacgta gtgacgctta
     1741 gcgccgcact gagttccggc ggcccgaccg ggatcgtctc ctacgcgtgg cagcaaatcg
     1801 ccgggccgag cgtcacactg gcggattcct caggggtcaa cgcggcgttt gtcgcccccg
     1861 cggccggagg cacactgcaa tttcaggtga ccctgaagac cgccgacggt cagacggcga
     1921 ccagcgtcgt caccgtgacg gtgcaaccgt ccggcaccgc gagaacggtg ctcgtgctcg
     1981 agagcgaccc gggcacaacg atgacctttg gtcaaaacct cgtgctgtcg gatgttctga
     2041 gtccgacgat aacttcgatc tccggaaatg gcctcaacgt gcatcttgcc gatgcgtacg
     2101 atacggagct ctacctttgg gcgccgtttg gccagccgct cgcagtaggg accgtctatg
     2161 agggcggacg gatgttcaac ggagagaaca gccatcgttt ggccggcttt cccggcctcg
     2221 aattcgagtt cgacagcttc tattgtacgg cggcatgggg tcgcttcgtc gttcgcgaac
     2281 tcacgactga tgcctccggg accgtcacgg cactggccgt cgacttccag cagacttgtg
     2341 tcccccaaaa cggctacgtg cggggctggc tccgggtcaa cagcaccgtt ccagtcggcg
     2401 acggtttgcc gctggcttcc gccggtccgg acctcgccgt tcaggcgggc agaacgatcg
     2461 atgtcgacgg ccgagcgagc tctgcgggac cgacgagtca ggcgacctac cagtggcggc
     2521 agctatccgg cccgccggtg cagttgacgg gcaccacgac gcc
//
LOCUS       DATLVW010000546         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_150175, whole genome shotgun sequence.
ACCESSION   DATLVW010000546 DATLVW010000000
VERSION     DATLVW010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150175"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VNN25_16240"
     CDS             complement(<1..898)
                     /locus_tag="VNN25_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93133.1"
                     /translation="MVEVRNQKAEGRSNRSRWFVFLLLLSALSFLPSCTGSKPEVTPP
                     PAPTPVTPAPEPAPAPVVEPPKLPTLDEVRALRTNQQLDLYESGLKSLKQSPDPITQR
                     RALALLALFYVDQKRTAEALPALAQAADADPLAAPWLRLRIVGIDADAANWPDAILNA
                     NRIIQDTPASSAATIARLRLPAFYAASGDAANTNTSFQIANGIGIDALTEEDFVALAK
                     SMAKASRMDLATPLRMRLLTEFTQGRFTEDTYDHVSMATPSPLDALSFDDELKIAQQL
                     GRNDRYGRAFDLLARIASRFPNE"
     gene            complement(898..1257)
                     /locus_tag="VNN25_16245"
     CDS             complement(898..1257)
                     /locus_tag="VNN25_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH93134.1"
                     /translation="MKGQKLFVRPFEAGDAAVLRAFASQHGGDPAPSAGIIGKLVGEL
                     AAVLTMEIEPDAIRILDLVVAEELRRKRVARVMLNELGILAAQMNREWLVAGVAHRPF
                     LERVGFHEADGLMKRRV"
     gene            1434..2408
                     /locus_tag="VNN25_16250"
     CDS             1434..2408
                     /locus_tag="VNN25_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXH93135.1"
                     /translation="MNVQEAIADFLQHGQVVRNLSDRTIRAYQSDLSQFQVHVDGAAM
                     VDITPDHLEQYLDKLGTGPYRDTSIRRKVAALKVFFRYLEEKGMFPESPARRLKIRKP
                     VENRVPTVLSSREVRALLSAPKDQIGALAGTRDHSAGGRNRYFCAIRDNVILELLFST
                     GIRIGELVALNVTDVGVENNQIHIIGRATRGRTVTITSHVVIEALMNYLEMRSERSLT
                     NDALFVGRSGTRLTIYSIENIFKKHVRLAEIKRHITPHALRHTMAAMLVSSGADIRDI
                     QEILGHASILSTQVYTRLPIQKRRSHTPADPARILVEPHSTRLSIKKT"
     gene            complement(2468..>3399)
                     /locus_tag="VNN25_16255"
     CDS             complement(2468..>3399)
                     /locus_tag="VNN25_16255"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HXH93136.1"
                     /translation="IYIATGHYDSINSDNSNATLDAPGADDDASGVAVIMELARVLSQ
                     HPLEATVMLVAVQGEEQGLLGSRGLAEEAVAKKWNVEGMITNDIVGGAVGGEGDIDNH
                     TVRIFSANPKGAGEGSARHWARFVRDGARVWLPHVNARLIYRLDRFGRGGDHTPFFEK
                     GFPAIRFSEANEDYKHEHQTVRVENGIQYGDLPQFVSPDYMRVVASVNAVALVTAACA
                     PAAPRNVKASGAVTNDTTMSWDMGTDTDLAGYEIVVRETTSDDWEKVIPVGKVSRYTL
                     KDFTIDNVFMGVRAIDRDGNRSPVRTPEERLPQ"
BASE COUNT          626 a         1081 c         1062 g          630 t
ORIGIN      
        1 tctcattcgg aaagcgggag gcgatgcggg cgagcagatc gaaggcgcgg ccgtagcggt
       61 cgttgcgtcc gagttgctgc gcgatcttca gctcgtcgtc gaaggaaagc gcatcgagcg
      121 gcgacggcgt ggccatcgag acgtggtcgt aggtgtcttc cgtgaagcgg ccttgcgtga
      181 actcggtgag gagccgcatg cgcaggggcg tggccagatc catccgactc gcttttgcca
      241 tcgacttcgc cagcgccacg aaatcctcct cggtcaatgc atcgattccg atgccgttcg
      301 cgatttggaa ggacgtgttc gtgttcgcgg cgtctccgga ggccgcatag aacgccggca
      361 ggcgcaagcg ggcgatggtc gccgcggagc tggctggcgt gtcctggatg atgcggttcg
      421 cgttgaggat ggcatccggc cagttggcgg cgtcggcatc gatgccgacg atgcgcagcc
      481 gcagccaggg agcggcaagc ggatcggcat cggcggcttg cgcgagcgcc ggaagcgctt
      541 cggcggtccg tttttgatcg acatagaaga gcgcgagaag cgcgagcgcg cgccgttgcg
      601 tgatcggatc aggcgattgc ttcagcgact tcagaccact ctcgtataga tcgagttgtt
      661 ggtttgtgcg cagggcgcgc acttcatcga gcgtcggcaa tttcggcggc tcgacgacgg
      721 gagcgggcgc aggctcggga gcaggggtga ctggagtggg cgccggcggg ggagtgactt
      781 ccggtttcga tccggtgcac gaaggcagaa aactgagagc agaaagcaga aggagaaaaa
      841 cgaaccaccg cgaccggtta cttctgcctt cagccttctg atttctgact tccaccatca
      901 caccctccgc ttcatcaggc cgtctgcttc atgaaaaccg acacgctcca ggaatgggcg
      961 atgcgcgacg ccggccacca gccattcgcg attcatttgc gctgccagga tcccgagctc
     1021 gttcagcatg acgcgtgcga cgcgtttgcg ccgcaactct tcggcaacga cgaggtcgag
     1081 gatgcggatc gcgtccggct cgatctccat cgttagtacc gcagcgagct cgccgacgag
     1141 cttgccgatg atgccggcag aaggcgcggg atcgccgccg tgctgcgatg caaaagcgcg
     1201 aagtactgcg gcatcgccag cctcaaacgg ccggacaaag agcttctgac ccttcatctt
     1261 gctgtcaacg gacgcgcaca atcgacgcgc ccgccatccc tcaaacccgc tcctgtcatg
     1321 cttttacagc ataccaagct cgggcaaata atacttgaca atcaaaggag gggaactaca
     1381 ctgccggaat cctcccagca gccaatttaa caattattct gtgagtgtga atgatgaacg
     1441 tgcaagaggc gattgctgat tttctgcaac acggacaggt tgtacgcaat ctctccgacc
     1501 gcacgattcg cgcctaccaa tccgacctca gccagttcca ggtccacgtg gacggcgcgg
     1561 ccatggtcga catcacgccc gatcacctcg agcaatatct cgacaaactc ggcaccgggc
     1621 cgtaccgcga cacgtcgatc cgccgcaagg tggccgctct caaggtcttc ttccgttacc
     1681 tcgaagagaa ggggatgttt ccggaatcac cggcgcgaag gctgaagatc cgcaagccgg
     1741 ttgagaaccg tgttccgacg gtcctttcct cccgcgaagt gcgcgccctg ctctccgcgc
     1801 cgaaggatca gatcggcgcg ctggcgggta cacgcgatca ctccgccggt ggccgcaacc
     1861 gctatttctg cgcaatacgc gacaacgtca tcctcgagct gctcttctcc accggcatcc
     1921 gcatcggcga gctggtggcg ttgaacgtga ccgacgtcgg cgtcgagaac aaccagattc
     1981 acatcatcgg ccgcgccacg cgcggacgca cggtgacgat cacgtcgcac gtcgtcatcg
     2041 aagcgttgat gaactacctg gaaatgcgca gcgagcggag cctgaccaac gatgcgctct
     2101 tcgtcggccg gtccggaacg cgcctgacga tctattcgat cgagaacatc ttcaagaaac
     2161 acgttcggct cgccgagatc aagcgccaca tcacgccaca cgcgctgcgt cacacgatgg
     2221 cggcgatgct ggtctcctcc ggcgccgaca tccgcgacat ccaggaaatc ctcgggcacg
     2281 cctcgatcct ctccacgcag gtctacacgc gcctgccgat ccagaaacgc cgcagccaca
     2341 cgcccgccga tcctgcccgc atcctcgtcg agccgcactc gacgcggctg tcgatcaaga
     2401 agacgtaggc gcgtctgatc ggaacgccgc cgtctcggcg gctggcatgg cggcgtctcg
     2461 ccgccagcta ttgcggcagc cgttcctcgg gcgtgcgtac cggcgagcga ttgccgtcgc
     2521 gatcgatcgc gcgaacgccc atgaaaacgt tgtcgatcgt gaagtctttg agcgtgtaac
     2581 ggctgacctt tcctaccggg atcaccttct cccagtcgtc ggacgtcgtt tcgcggacca
     2641 cgatctcgta gccggccaga tcggtgtccg ttcccatgtc ccagctcatc gtcgtgtcgt
     2701 tggtcacggc tccggaggct ttcacgttcc gcggcgcggc cggagcacag gcggcggtga
     2761 cgagggcgac tgcgttgacc gaggcgacga cgcgcatgta gtcgggggag acgaactggg
     2821 ggaggtcgcc gtactggatg ccgttctcca cgcgcacggt ctggtgctcg tgtttgtagt
     2881 cctcattcgc ctcgctgaag cggatggcgg ggaagccctt ctcgaaaaac ggcgtgtgat
     2941 cgccgccgcg gccgaatcga tcgagccggt agatcaggcg cgcgttgacg tgcgggagcc
     3001 agacgcgcgc gccgtcgcga acgaagcgtg cccagtgccg cgccgatcct tcgcccgcgc
     3061 ccttcgggtt cgccgaaaag atgcgcacgg tgtgattgtc gatgtcacct tcgccgccga
     3121 ccgcgccgcc gacgatgtcg ttcgtgatca tcccctcgac gttccacttc ttcgccacgg
     3181 cttcttcggc cagccctcgc gatcccagca gtccctgttc ttcgccctgg acggccacca
     3241 acattacggt ggcctcgagc ggatgctgcg aaagcacgcg cgccagttcc atgattacgg
     3301 caacgccgga tgcgtcgtca tcggcgccgg gggcgtcgag cgtcgcgttc gagttgtcgg
     3361 agttgatcga atcgtagtga ccggtggcga tgtagatac
//
LOCUS       DATLVW010000547         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_358153, whole genome shotgun sequence.
ACCESSION   DATLVW010000547 DATLVW010000000
VERSION     DATLVW010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358153"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1186
                     /locus_tag="VNN25_16260"
     CDS             176..1186
                     /locus_tag="VNN25_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXH93137.1"
                     /translation="MIDFESAASHLLLNPRMPAEERERFERMTPALEAHVFVATSGST
                     GAMKLVALSKRAILASAAAVNERLEVTDADVWAAVLPTFHVGGLGVYARCHLAGARAL
                     PMDWNAQDFAESEATLASLVPAQVHDLLAERLTPSQSLRAILVGGGAFDEVAVARARE
                     AGWPVLPSYGMSECCSTIAIEDVLLPHLEARVEPDGRLAFRGSSLLTGYVANEGLTDP
                     KIDGWFISEDAGIIDGRTLRIHGRASDFVKIGGESVDLGRLDRILRELAGDSAAIVAM
                     PDERLGCVIHLASTVDADAVVDAFNARVHPFERIRAVHRVDAIPRSPLGKLLRAKLAS
                     MI"
     gene            1226..2965
                     /locus_tag="VNN25_16265"
     CDS             1226..2965
                     /locus_tag="VNN25_16265"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXH93138.1"
                     /translation="MRKRTALLIAIAAGIAVMFLTASDAVDALELPVRDLALRLLPPR
                     DATMTVVVGIDEDSLRAIGPWPWERAVLAAIVNRAADAGARGVVLDILLADGRAGDDQ
                     LAAAARRVPTLAVSALDEQGQWHLPVPALAAAMTPVHGNFEIDHDGIVRRFSATKQNE
                     DRSLVALSVEASSLITGAPVPVGRTLAPAFRTRPGRIPMISAAALIRDGSGASQLRGK
                     LVFVGPVAIALGDRVLTPTSSRRVANPGVTVHAAATESLIRGDVIRELAPISSGGFTL
                     ISVGALAILRRRRIVAIAAIIVIVAGGLLLLGNGIAVPFATMLASVILTSAAFEAQTM
                     IAALASSEARFLVQRDRDAESKRVLAHELKTPLASMRNLTQLLGLFELSDAERQRVTS
                     LLEQEAGKLETMVGTLLDLERLPLRDFAVSSSIVDLGALATARVDFLRASTDRTLLVS
                     AEPGLLVRADAALIERVIDNLVGNALKYTSGSVSVTVTRRGHDAVLQVEDRGPGIAEG
                     DRARIFERFVRGAAAAGTQGLGLGLSLVAEIARWHGGEAGVEAVPSGGSRFSVTLPVT
                     LHGAVSEPMTGAM"
     gene            2965..>4107
                     /locus_tag="VNN25_16270"
     CDS             2965..>4107
                     /locus_tag="VNN25_16270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH93139.1"
                     /translation="MASVLVVDDDAGIRESAAMALEKVGLKTFQAGDTVAALQMLRAH
                     RIDLVLSDIYMPGETGITLLQAISERRDPPRVILMTARGSIETTALAQRFGAFDYLAK
                     PFDLNDLIARVRAALNVSTAEGVEVEPGPPSRMVGSHPLMIAMYKAIARVAPLNVPVL
                     ILGESGSGKELVAQSIHDLGARVAGPFIAINCGAIPDSLLENELFGSTRGAFTDSRQD
                     RRGALSRADGGTLFLDEIGDISPAFQVKLLRFLQDGVVTPLGSEKSHRVDVRLIAATH
                     RDIKRLVGEGKFREDLYYRLAGYEIAVPPLRERASDIPLLVDFFRHRIAEEMKRPSIP
                     GPSQSVLDVFAGHGWPGNVRELEQVTRRIVIDSGALLDTAAASRALR"
BASE COUNT          642 a         1361 c         1324 g          780 t
ORIGIN      
        1 cgcaaaaagc gcggggggag aaggcgctcg atggaggagc agggcgactc caaccatcgc
       61 gcgtggcggc taacgtagcg ccccgaacag acgtcgcacc gcgagcagac gtagcgccgc
      121 cggcccggcg gctgtatcgc cggcggctcg ccggcgattt tggcgcccaa cgcccatgat
      181 cgacttcgaa tccgccgcgt cccaccttct cctcaatccg cgcatgcctg ccgaggagcg
      241 ggagaggttt gagcggatga cgccagccct cgaggcccat gtcttcgtcg ccacttcggg
      301 atcgaccggc gccatgaagc tcgttgcgct atcgaaacgc gctatcctcg cttccgcggc
      361 agcggtcaac gaacggcttg aagtaaccga tgccgatgtc tgggccgccg tccttccgac
      421 gtttcatgtc ggcggtcttg gcgtttacgc gcgatgtcac ctggccggcg cgcgcgcact
      481 gccgatggat tggaatgcgc aggactttgc ggaatcggag gccacgctcg cctcgctcgt
      541 gcctgcgcag gtgcacgacc tcctcgctga gcgcctcacg ccatcacaga gccttcgcgc
      601 cattctggtc ggcggcggtg cgttcgacga ggtcgccgtc gcgcgtgcgc gcgaagccgg
      661 atggcctgtc cttccgagct acgggatgag cgaatgctgt tcgaccatcg cgatcgaaga
      721 cgttctcctt cctcatctcg aagcgcgcgt cgaacccgac ggccgattgg cgtttcgtgg
      781 atcatcgctg ctgaccggat acgtggcgaa cgaaggattg accgatccaa agatcgacgg
      841 ttggttcatc tccgaggatg ccggcatcat cgatgggcga acactacgca tccacggacg
      901 cgcctcggac ttcgtgaaaa tcggcggcga gtccgtcgac ctcgggcgcc tcgatcggat
      961 tctccgagag ctggcaggag attccgctgc catcgtggcg atgcccgacg agcggctggg
     1021 ttgcgtgatt catctcgcat cgacggtcga tgctgacgcg gtggtcgatg cgttcaacgc
     1081 gcgcgtccat ccgttcgagc ggatccgcgc ggttcatcga gttgatgcca ttccgcgatc
     1141 gccattaggg aagcttttgc gcgcgaaatt ggcgtcaatg atttgatgca acgtcgccct
     1201 cgttcctgcg tcgtaccata cgaacatgcg caagcggacg gcattactca ttgccatcgc
     1261 ggctggaatc gcggtgatgt ttctgaccgc gtccgacgcg gtcgatgctc tggagttgcc
     1321 cgttcgcgat ctcgcattgc ggctgcttcc gccgcgagac gccacgatga cggtcgtcgt
     1381 cggtatcgat gaagattcgc tgcgcgcgat cggtccgtgg ccgtgggaac gcgctgttct
     1441 tgccgcgatc gtcaatcgcg ctgcggatgc cggcgcgcgc ggcgtggtgc tcgacatcct
     1501 tctcgcggac gggcgtgccg gcgatgacca gctcgccgcg gcggcgcggc gtgtgccgac
     1561 gctcgctgtc tccgcgctcg atgaacaggg ccaatggcac ctgcctgtgc ctgcactggc
     1621 cgcggcgatg acgcctgtgc atggcaactt cgagatcgat cacgacggca tcgtgcggcg
     1681 tttctccgcc accaaacaga atgaagaccg ttcgctcgtg gcgctttcgg ttgaagcgtc
     1741 ttcgctgatc accggcgcgc cggtgcctgt cgggcgcacg ctcgcgccgg cgttccgaac
     1801 gcgtccgggc aggattccga tgatcagtgc cgccgcactg attcgcgacg gttcgggagc
     1861 gtcgcaactg cgtggcaaac tcgtgttcgt cggccctgtg gcgattgccc tcggcgaccg
     1921 cgtgctcacg cctacttcct cgcggcgagt cgccaatccc ggcgtgaccg ttcatgcggc
     1981 agccaccgaa tcgctcatcc gcggcgacgt catccgcgag ctggcgccga tctcctcggg
     2041 cggcttcaca ctaatctccg tcggcgcgct tgccatcctt cgacggcgcc ggatcgtcgc
     2101 catcgccgca atcatcgtga tcgtcgcagg aggccttctg ctcctcggca acggaattgc
     2161 cgtaccgttc gcgacgatgc tcgcctccgt catcctcacc agcgccgcgt tcgaagcgca
     2221 aacaatgatt gctgcgctgg ccagcagcga ggcacgtttc ctcgttcaac gggatcgcga
     2281 tgccgaatcg aagcgcgtcc ttgcgcacga gctgaagacg ccgttggcgt cgatgcggaa
     2341 cctcacccag ctactcggtc tgttcgagct ctccgacgcc gagcggcagc gcgtgacgtc
     2401 cctcctcgaa caggaggctg gcaaactgga gacaatggtc ggcactctgc tcgatctcga
     2461 gcgcttgccg ctccgcgact tcgcggtctc ctcatccatc gtcgacctcg gagctctcgc
     2521 tacggcacgc gtcgactttc tccgcgccag cacagatcgc actctgctcg tgagcgcgga
     2581 gcctggacta ctcgtccgcg cggacgccgc gttgatcgag cgggtcatcg acaatctcgt
     2641 cgggaacgcc ttgaagtaca cgtccggctc cgtcagtgtg accgtgactc gcaggggcca
     2701 cgacgccgtt ctccaggtcg aagatcgcgg tcctggtatc gccgaggggg atcgcgcacg
     2761 gatcttcgag cgctttgttc gtggcgcagc cgccgcagga acgcagggac tcgggcttgg
     2821 cctttctctg gttgctgaga ttgcgcgatg gcacggcgga gaagcgggcg ttgaggccgt
     2881 gcccagcggc ggctcccgct tcagcgttac tctgcccgtt actttgcacg gggcggtgtc
     2941 tgagccgatg acaggagcga tgtgatggcc tcggtgctgg tagtcgatga cgatgccgga
     3001 atccgcgaga gcgccgccat ggccctcgag aaagtcggct tgaagacatt ccaggcgggc
     3061 gacaccgtgg ccgccctgca gatgcttcgc gcgcatcgca tcgacctcgt gctcagtgac
     3121 atctacatgc ccggcgagac cggcatcacg ctgctccagg ccatctccga acggcgcgat
     3181 ccgccgcgcg tgattctgat gacagcgcgc ggctcgatcg agaccaccgc tctggcgcag
     3241 cgcttcggag cgttcgacta cttagccaaa ccgttcgatc tcaacgatct catcgcgcgc
     3301 gttcgtgcgg cgctcaatgt cagcactgcc gagggagtcg aggtcgagcc ggggccgccg
     3361 tcgcgtatgg tcggctcgca tccgctgatg atcgcgatgt acaaggccat cgcgcgggtg
     3421 gcgccgctca acgtgccggt gctgatcctc ggcgagagcg gcagcggcaa agagctggtt
     3481 gcccaatcga ttcacgacct cggcgcgcgc gttgccggcc ccttcatcgc catcaactgc
     3541 ggtgcgatcc ccgattcgct gctcgagaac gaactcttcg gatcgacccg aggagcgttc
     3601 acggattcga ggcaggacag gcgtggtgcg ctttcccgcg cggacggcgg gacgctcttt
     3661 ctcgacgaga tcggcgacat ctcgccggcc ttccaggtca agctgctccg ctttctgcag
     3721 gacggcgtcg tcaccccgct tggttccgag aagagccatc gcgtcgatgt gcgcctcatc
     3781 gccgcgacgc accgcgacat caaacggctc gttggcgagg gaaagtttcg cgaggacctc
     3841 tactaccggc tggcgggcta cgagatcgcg gttccgccgc tgcgcgagcg cgcatccgac
     3901 atcccactgc tcgtcgactt cttccggcat cgcattgccg aggagatgaa gcggccgtcg
     3961 attcccggcc cgtcgcagag cgtgctcgat gtctttgccg gacacggttg gcctggcaac
     4021 gtccgcgagc tcgagcaggt cacgcggcgg atcgtgatcg attcgggtgc gctgctggac
     4081 accgcggcgg cgtcgcgcgc gctgcgc
//
LOCUS       DATLVW010000548        22354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_602256, whole genome shotgun sequence.
ACCESSION   DATLVW010000548 DATLVW010000000
VERSION     DATLVW010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22354
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602256"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1715)
                     /locus_tag="VNN25_16275"
     CDS             complement(57..1715)
                     /locus_tag="VNN25_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXH93140.1"
                     /translation="MRRASRIVILLLCSGLLLAGASRVEAQDAFAGVWRGGNDPYYLW
                     IGTNFNDFKAKWESLSAQNLRLVDLEIYNQGGQQKFMGVWRGGNDAHYLYVGVEWNEF
                     KAKWDELTKQNLRLTIVRTYVEGGRRKYAGVWRAGSDAYYLWAGVDWNSFKAKWDELN
                     NQGLRLIDMETYVEGFSRKYIGVWRAGNDAYYLWAGVDWNSFRAKWDELKKQNLRLTV
                     LKTYEESGQRKYTGVWRGGSDAYYLWVGADYENFTSKWHELVGQHLRLVSVATYPGCG
                     DCANHVVSNKAYDYGITGHDTVYHWPVDVDGSNKWVRLSALEFSGAPFTLPFSNTNVK
                     RWNGWLYSPGSWHHAVDYAIDLNQTFPIKAAAAGKVIFIGWDNWSGNTIVISHNVGGK
                     VDAFRTIYMHMRNGPAADCAHAWSGTVPTLSGDTLTKYKGHLNASGCLSNPGSRNPSV
                     TNWGTNAQTIAVAVNQQVTAGQVLGWAGDTGPGGNGGSDATANTHLHIFFTVKDPTNG
                     QFYFFDPYGIYGYPSCYPTATTGSAGGVCARYPIAWKGGAPQYP"
     gene            complement(1759..2970)
                     /locus_tag="VNN25_16280"
     CDS             complement(1759..2970)
                     /locus_tag="VNN25_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006885490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH93141.1"
                     /translation="MTANGMTRTRALLLVGIAELLGMSLWFTASAVAPRIASEWKLSA
                     GTTAWLTLAVQLGFVAGTLISAMANLPDVMSVRRLFAVSALAGAITNALFAWLARDAM
                     MAIALRFLTGVFLAGVYPPGMKIIATWFREGRGFALGVLVGALTLGKASPYLINAVGS
                     QSWRINVAFASLLAAAGGLIVLFFVSDGPFALPNQPFDLGQAADIVRNRAVRLANFGY
                     FGHMWELYAMWTWIPVMLRASMIMSGSPARLAEVGSFIVIGSGAVGCVIAGRFADRIG
                     RTIVASAAMIASAACCVAIGFLFGGPPIALLAVAAIWGATVVADSAQFSACVTELSDP
                     RYIGTALTLQTCIGFLITTASISIMPRIEHALTWHWAFAILAPGPILGTMAMLRLRAL
                     PEAKRIAQGRR"
     gene            complement(2978..3841)
                     /gene="cdd"
                     /locus_tag="VNN25_16285"
     CDS             complement(2978..3841)
                     /gene="cdd"
                     /locus_tag="VNN25_16285"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF006537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HXH93142.1"
                     /translation="MLTQEQLASIESARQAAGGFLPAGEVARIIAGGMSIEQLMLDLI
                     PLAKTFAIPPISNFFVGVVALGSSGSLYFGANYEFPGHALTFSVHGEQAATVQALSNG
                     ETGVEMLAISAAPCGYCRQFLNELTTAPTLKIILPDTPATPLTALLPAAFGPIDLGVT
                     GGLMSPRSHGMTLAESTNDEVARAALDAANRSYAPYSASYAGVALKTSDGGIVTGSVA
                     ENAAFNPSMSPLEAAVVNLVIGAGRKYIDIVDAVLVEARDSKASQSGVTREILGSITQ
                     LPLRIYEAVAP"
     gene            3898..4239
                     /locus_tag="VNN25_16290"
     CDS             3898..4239
                     /locus_tag="VNN25_16290"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXH93143.1"
                     /translation="MASKRRAVTFDLIRDLALKHPGVEEGTSYGTRALRVKGKFMARL
                     KEDGDSVAFRISFDERDLLMQTKPHAFYITDHYLGYPAVLMRMSAVTPEEAADIVAMS
                     WRMVAPKKLKT"
     gene            4344..5372
                     /locus_tag="VNN25_16295"
     CDS             4344..5372
                     /locus_tag="VNN25_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXH93144.1"
                     /translation="MHRKMMQWVLWTLILLSLATPAFSQTLRAHLQHVFRGHADVGRQ
                     VAFSPDSQILATSSADRTVKLWRLSDDKLLRTLLHPEGVTSIAFSADGQWLVSGSYDH
                     NVRIWRVGDGALMRTLKGHEGVVWSVAFSPDGQRVVSGGEDKTVRVWRCADGALLRTL
                     RGHTLNVWHVAFSPDGSLIASGSFDKTIRIWRSDSGSLVRTLTGSKEAVVGIDFSPNG
                     QMLASGGDDSSIRIWRVKDGALLRTIETPNHVYSVAFSADGQWLASGGRERGALGTLW
                     KQIAGPGSTHGTTVRLWRVHDGMLQQELAGHADEVMYVALSRDGKWLASSSEDKTVGL
                     WRLQTSGR"
     gene            5415..6416
                     /locus_tag="VNN25_16300"
     CDS             5415..6416
                     /locus_tag="VNN25_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXH93145.1"
                     /translation="MKISYARGLSLLLLGPATVAQARVVARHVAQPFTAVATNDATKT
                     PALTSASKPGPAILRAQILLDRAHFSPAEIDGRYGSNMRSAVAAFNASRKIPSGSNVS
                     AATWKALNADTAPTITPYTITAADVTGPFAPIPTETADKAKLAALGYTSLAEELGERF
                     HVSPALLQKLNHGIAFDKAGAVIQVPNVARPPLPKTAGMSIRVSKSRQAVEAIDASGN
                     VLATYPATIGSVHDPLPIGKWKINGVEWNPKFNYNPDLFWDAEPTDKKAKLPPGPNNP
                     VGVVWIDLSKPHYGIHGTPDPSTIGKTTSHGCIRLTNWDASELAKIVTPGMPAVLEQ"
     gene            6413..7039
                     /locus_tag="VNN25_16305"
     CDS             6413..7039
                     /locus_tag="VNN25_16305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXH93146.1"
                     /translation="MRVAKKMGWAVGVAGVAGLAWMASGTTQSVIAPAPAFRQATRTN
                     ASLPTVPVTPRMLLTPGEGPRPLTMPVEGIDPSTIHDTFDEMRGGDTRRHDALDILAA
                     RGTNVVATDDGTVKKLFTSVAGGLTVYQYDPEQRYCYYYAHLDAYAMGLHEGQILKRG
                     DLIGYVGTTGNAPKDTPHLHFAVIKLDPDRRWWTGTAIDPYPLLYRPR"
     gene            7183..10479
                     /locus_tag="VNN25_16310"
     CDS             7183..10479
                     /locus_tag="VNN25_16310"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXH93147.1"
                     /translation="MFHNPFPIPIRFGQGGTIDIATGDFNGDHKPDILIINAADNLEV
                     LLNDGNGSFAPSVSSPFSGYFDSWAIDDVNGDGKLDFAFSDGIHTVKVLLGNGDGTFG
                     VEHPFTTVQPVHKVALADINGDHHLDLVVGADASVTGMIGVYFGDGTGQFSNSGVITN
                     CNCDLTTLTVADHNGDGKLDITVGNVFLAGNGDGSFQPPVYLTAPPFTGTIADFDADG
                     IADHLWCEEGGSVVSIQRGRNDGSFTESALFVSGPSAFKSIAADFDDDGKVDVVTLDY
                     NLEIPALSLLRGNGDGTFQAPRSYRVGRTAPEPNFGQVRSLAVAADMNGDGKPDIVAT
                     PMHVNPPPFGELAVMINDGTGKMAAPLYTNLGQTAGGAFAIGDLNGDGKADAVVAGNP
                     AEVLMGNGDGTFSAPVHLNFAVNNTPQLVDLDGDGKLDLFFDQGIPFIVYHGNGDGTF
                     SAMSTVTFPIGVALFGDVNGDTYPDIVTNAGAGIVVALNDGHGAFSPHSPLPGFATDP
                     VALADFNGDGKLDILSTGTSVRLGNGDGSFGAPIDFPVSPTPFGPVRTADFDRDGKMD
                     VAFGTTVLFGDGSGRFSRYARFRTTWVTALDVADIDGNGSPDLVVTNSIADDVDILLT
                     YGSPAPVAQPGLALTSDKPSPQYGEAVTITATMSGGTTPLTGAVTFSIDGIPVAMSIL
                     DPTGIATFRSGYRVGSFVVTATYSGDENYLSATNSLNLNVSKALTSISVSAFPNPARL
                     HDGVRIVASMSYPVPSAVPGPSGSLILREGTTTLKTSSFSMNTIVSTLPLGTHTLTVD
                     YAGDANYQASTASYEQVIIKPLLFMSISTSPSQPIAGTDVTVRASCTVNATGTISFWI
                     NGAAAGSAPIVQGIAEITATMKLGLNTVHVTYPGDGTYDSSTADQNITGYISQTGTPV
                     IAAKGSAFLGQGDISVQWSQVVNATSYTIYRRTSIGSGWEAYKTFSGNTSGYSDTMPI
                     NTCWLMAITAQTPSGTTPMSAPDLVTSAQFSNVVAPGTAIKALDLTETRTAVNLVRAF
                     ANLPAVAFTNTVAPGERIAALDVMQLRSGLSEARAAIGLPLGFSDPQLAPGTSIIRAA
                     HVLELRAGVD"
     gene            10550..11269
                     /locus_tag="VNN25_16315"
     CDS             10550..11269
                     /locus_tag="VNN25_16315"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXH93148.1"
                     /translation="MILVLLLMTVPMFAEDAPAPPPKPWTSSIGAGLAITSGNTDTKN
                     YNLAFATKYDPKTRLVFKADALYLRGDSGGVTQVDKASADAREEYALSDRTFAFAEVS
                     YLRDPFKDINYFVAPLAGGGYRIIKSDARNLTVDGALGAEFESNAGAGRTSGGAAKAG
                     ENFDWTLSPTSKITQKLTAIWKTSDFGDALYHFDAGLTTTVATRLELKLAWVYDYKTK
                     PPSSSVKKGDSALFAALLFKF"
     gene            complement(11281..11502)
                     /locus_tag="VNN25_16320"
     CDS             complement(11281..11502)
                     /locus_tag="VNN25_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH93149.1"
                     /translation="MARPLRLEFADALYHITSRGNERRAIFRTNRDRETFLTFLGEAT
                     LRFGWSVTAWVLMTNHFHLTDSVLRRSKV"
     gene            11643..13085
                     /locus_tag="VNN25_16325"
     CDS             11643..13085
                     /locus_tag="VNN25_16325"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXH93150.1"
                     /translation="MKPTRAQLEDVLASLDGSGLRYADIRWTATHEQHVRVRNGEVET
                     LASTIDRAIGVRVLVGNGWGFAANSDTSEESLRKTAKRALEVAAASNIASTQRVTLSD
                     IEPHVAAWSSTYEIDPWSIPLEKKIEHLLQSTEPMRGDARIHQVSGDISCYRQEKIFA
                     STVGSFIEQTTTEMGGGVEAVAIEGDEFQRRTYPNPFGGDYQAAGWEFIEQLNLPGKA
                     LEIRDEALALLIAPKAPAGRFDLIVGSAQLALQVHESCGHPTELDRAMGLEISLAGGS
                     FLQPSMLGNFRYGSDLVNIVGDATIPGSIGSFGFDDDGVPAQRFHLIEKGMFVGYLTG
                     RDTAPTIGRASNGTVRAETAARIPIIRMTNINLEPGTTPMADIIADTKRGIFVETNKS
                     WSIDDLRLNFQFGCEVAWEIENGRLGRLLRNPLYTGTTPDFWRSCDAVADRASWQIWG
                     LPNCGKGDPMQTMRVAHGAPAARFRNVEMG"
     gene            13169..14548
                     /locus_tag="VNN25_16330"
     CDS             13169..14548
                     /locus_tag="VNN25_16330"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXH93151.1"
                     /translation="MRNEHDSFAILDQALAAAGTAEADAVFISTDQNISRFANSNVHQ
                     NMSEISAELSLRVIVDNAMGVASTTVFDDAEIERTAALAREAARHADPLPNFSGLYRA
                     NEPLPSVRTFDDATANIAPAEKARALRTMFDRGVANGVHFAGVYGTGASSVAVANTHG
                     VRRYCTMTASDATVIAIGANGSGYATAVDRSNVDVTALGEEAAFKAALRADVIEDIAP
                     GPYDVILEPPATAEVLEWMTMIAFSGSAYEDGSSFFVGNIGNQVLGANFTLTDDALDN
                     AFLPFPFDIEGLPKRRVAIIERGIVRTPVVDKAYADRLGFAPTANGWSLGGSDHGSAL
                     HLSIEGGDATREELIRSTKRGIWVTRFNYVNGLLEPRTALMTGTTRDGTFLIRDGEIV
                     ARLPNLRWTQSIVEAFSRIEGLTRERRRVGTWYNHFGGTIAPVMKIAGWNFTGKQQTG
                     GMAQSSLGD"
     gene            14635..15558
                     /locus_tag="VNN25_16335"
     CDS             14635..15558
                     /locus_tag="VNN25_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93152.1"
                     /translation="MSQYILRRFLQMIPIAFGILTLVFSLIHMIPGDPAVQIAGEGAR
                     PEDVVAIRKALGLDQPLWTQYVHYVTHVIRGDLGTSFQTNTSVTEQIKTRYPATIELA
                     FGSMFVALLVAFPLGIISAIYRNSWIDNLARFFALIGVSMPSFWFGPLLIIAFAINHE
                     WFPVSGRDAGLRSLVLPSLTMGLALSAILTRMIRISLADELTQLYVTTAVAKGVTRAQ
                     AIFRHALKNALIPVITVLALQFGSLLTGAIITEQIFSWPGLGRLLLTSISTRDYPQVQ
                     ASILVIALTYILVNFASDLLYGVVDPRIKLE"
     gene            15684..16508
                     /locus_tag="VNN25_16340"
     CDS             15684..16508
                     /locus_tag="VNN25_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93153.1"
                     /translation="MKRFFGNFAFTSGLILTVALIVVALAAPLIAPFDPNQQDTGRRL
                     EAPSHEHLLGLDDLGRDVLSRIIWGSRVSLRVGFSVVILASIIGITLGAMAGYFGGWM
                     ETVIMRVTDILLSFPGILLAIAMVAVLGPSLNNVVIALATQGWVGYARLGRGQVLKVR
                     EMDYVVAAKALGARSPRVILRHVLPNIVNPVIVMATLGLAGAILSEAALSFLGLGVQP
                     PTPSWGAMLTSGRRYLGLANHLAIWPGVAIMLAVMGLNFLGDGLIDALDPKYRKEM"
     gene            complement(16512..18002)
                     /locus_tag="VNN25_16345"
     CDS             complement(16512..18002)
                     /locus_tag="VNN25_16345"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93154.1"
                     /translation="MRKHLAAIIAAVAIAQLWLAHRYFGFMTGDDVEVLSEAFRRARG
                     LDFHPWDIRNLFVPDVVVAPVVWIASRLGISDTRTLILASALPFIALTALTIVLVYRL
                     ALRWCEDERAALVASLLFAMHWIPLGFGSTVYPRTLAAACVVGAALIVERWPFAAGAL
                     AGLAFADRFSEIVFLVPLLVMITKHRQECLCHIEPSQVGSAHSGRSQELSAQTGSPQE
                     CSVQTVPSPECPIHARNGVAQTLLSVPWMQALKVMVGATVAISLTVGLYDWLTWGAPF
                     SSFIKFTHLTLVAPDFASRVKYQAPWWYIANLVRWCSPLLLPLLWYARRSRAIWFVII
                     PLLAFSAVRHKELRYLQVMIPFLAIAAAIGFASMYRSHRRIAIALVATSLIWDLHGMR
                     YFARKSQPAVEAAQWLGANPRVHSIAASQLWAFGDKLFLGDKMAMSDLGTPPEKLPEV
                     TADAVALYETDLDVPSRVEALRALGYTPVRTFRDDPARAVVVFMKK"
     gene            complement(17995..19986)
                     /gene="ligA"
                     /locus_tag="VNN25_16350"
     CDS             complement(17995..19986)
                     /gene="ligA"
                     /locus_tag="VNN25_16350"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXH93155.1"
                     /translation="MTPKKEIETLRADLERHSQLYYVYATPEISDFEFDQRLRRLQEL
                     EAQHPELFDPNSPSQRVGGAPMTGEFATVIHDPPMLSIENAYTLEELHEWDARVKKGL
                     GVEEVEYEAELKIDGVSIDLLYENGAFARGATRGDGVRGDDVTVNVRTVRGLPLKIAT
                     KLKRVEVRGEIYISSNDFALLNAQLEEAGQPVLANPRNAAAGSLRQKDPKLVAQRRLS
                     AFVYHLVATDDVRVTSQAQAYEILAAMGFPLNPKRDVFAGMAAVEAFIDTVREQRHDL
                     SFDIDGIVIKVNKRAQQQELGATSKAPRWAIAFKYPPEAAQTIVRAINLYVGRTGAVT
                     PVAEFDPVHIGGTKVVNASLHNFDEVGRKDIRVGDSIVVEKGGDIIPKVVDVLVDKRP
                     EGTVPVALPETCPACGEPLHRFEGEVAIRCINQGCPAIVRESLFHFGARKAMDIEGLG
                     YQTITQLLDAKLITDYASLYELREEDVAALPGKGELSARKLAEGIEKSKTAELSRVIF
                     AIGIRMVGERAAKLLAARFGSLDALMNATVDELVDVDEIGPKVAESITFYFSVPANRE
                     RIEKMQRLGVAPTHVAEVRGDLLAGKTLVVTGTLARFSRDEIHKLIEREGGKPSGSVS
                     AKTSYVIAGDAAGSKLDKAKSLGVPVLTEDEFLAMIGDA"
     gene            complement(20020..20496)
                     /gene="bfr"
                     /locus_tag="VNN25_16355"
     CDS             complement(20020..20496)
                     /gene="bfr"
                     /locus_tag="VNN25_16355"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXH93156.1"
                     /translation="MKGDQKIIDILNDVLTSELTAINQYFVHAEMCDNWGYQHLHDRI
                     RKHSIGEMKHAESLIERVLFLEGIPNMQRLFKVTIGETVPEQLSIDLALEVDSVKRLN
                     AGIETCREAEDNNSRHLLEEILEDEEEHIDWLEAQLSLIEQVGAPNYLAQQIHEHE"
     gene            complement(20522..20743)
                     /locus_tag="VNN25_16360"
     CDS             complement(20522..20743)
                     /locus_tag="VNN25_16360"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXH93157.1"
                     /translation="MIVCLCRAKNERDVLRAIEGGSETLADLQECGIGTDCGGCHNFL
                     RKMLSANESVSPVAFVPLPSTEVWSESVA"
     gene            complement(20845..21345)
                     /locus_tag="VNN25_16365"
     CDS             complement(20845..21345)
                     /locus_tag="VNN25_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93158.1"
                     /translation="MPDRTEVYPFRPWVARAGFSIVLVAAAVTAAILVALFVSPSARY
                     GWIGEHAVWAYIATLWIGGLKVWLGARRPIAEIGDETLTLRPLHQFRSRMVRWDALRG
                     TEQMLGGDRLIVYWDSPRGLRFVALNLNLIKGRRNFLAAVDERLRSMGFAEKTVERSR
                     YLTRPA"
     gene            21444..21866
                     /locus_tag="VNN25_16370"
     CDS             21444..21866
                     /locus_tag="VNN25_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93159.1"
                     /translation="MVGKLDGTGRRILVIDDDLAIRVLLQAVLKRMKFEVELAEDGAV
                     GLEKLKNDPLYDLILLDLMMPRVNGYEFIERVRREQKDAPPHILVFTAAGKRGVDKIP
                     PGAICNSILKPFDLEKFIDMIAECLDGPHKPHLAAPLS"
     gene            complement(21842..>22354)
                     /locus_tag="VNN25_16375"
     CDS             complement(21842..>22354)
                     /locus_tag="VNN25_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXH93160.1"
                     /translation="LADAHVTIATTSSRGRSTPSDLQPVGIAQLFSSMDDSQTLALVF
                     GRENSGLTSEEVRHCQRTAIIPTDPAFPTMNLAQSVGVFCYELSTTSRAATPRDLAPA
                     VMIERLHERVQAFLAEVGYLLGDNPDHIYDDLRLIAARADLDAREVTILLGIIRQMEW
                     KLAQERGAAR"
BASE COUNT         4303 a         7176 c         6729 g         4146 t
ORIGIN      
        1 caactctgtg tcctcagtgt ctctgtggtt gattcttttg tccgaccgtc cgcccatcac
       61 gggtactgcg gcgcgcctcc cttccaggcg atcggatagc gcgcgcagac gccgcctgcc
      121 gatccggtcg tcgcggttgg atagcaactc ggatagccgt agattccgta cgggtcgaag
      181 aagtagaact ggccgttcgt cggatccttc accgtgaaga agatgtgcag gtgcgtgttc
      241 gccgtcgcgt cgctgccgcc gttaccgccg ggaccggtgt cgccggccca tccaagaacc
      301 tgacccgccg tcacctgctg attaacggca acggcgatgg tctgggcgtt cgttccccag
      361 ttcgtcacgc tcgggtttcg gcttcccgga ttcgacaggc agccgctggc attgaggtgg
      421 cccttgtact tcgtcagagt gtcgccgctc agcgtcggga cggtgccgga ccatgcgtgc
      481 gcgcagtcgg cggccgggcc gttcctcatg tgcatgtaga tcgtgcggaa ggcatccact
      541 ttcccaccga cgttgtgact gatgacgatc gtatttccgg accagttgtc ccagccgatg
      601 aagatgacct tccctgccgc cgcggccttg atcggaaacg tctgattcag gtcgatcgcg
      661 taatcgacgg cgtggtgcca tgagcccggg ctatacaacc agccgttcca gcgcttcacg
      721 ttcgtgttgg agaacggaag ggtgaatggc gcgcccgaaa actcgagcgc ggagagacgc
      781 acccacttgt tgctgccatc gacatcgacc ggccagtggt agacggtgtc gtgaccggtg
      841 atgccgtagt cgtacgcctt gttggaaacg acatggttcg cgcagtcgcc gcagcccgga
      901 tacgtggcca cgctcacgag gcggagatgc tgccccacca gttcgtgcca cttggacgtg
      961 aagttctcgt agtcggcgcc gacccacagg tagtacgcat cgctgccgcc gcgccagacg
     1021 cccgtgtact tgcgctgtcc ggattcttcg taagtcttga ggactgtgag ccgcaggttt
     1081 tgtttcttca actcatccca cttggccctg aagctgttcc aatcgacgcc agcccagaga
     1141 tagtaggcgt cgttgccggc gcgccacacc ccgatgtact ttcgggagaa gccttcgaca
     1201 taagtctcca tatcgatcag gcgcagaccc tgattgttca gctcatccca tttggccttg
     1261 aaactgttcc aatcgacgcc ggcccagaga tagtaggcat cacttcccgc acgccagacg
     1321 ccggcgtatt tccgtctgcc accttcaacg taggtcctga cgatcgtgag ccgcaggttc
     1381 tgcttcgtca gctcatccca cttcgccttg aattcgttcc actcgacgcc aacgtagagg
     1441 tagtgcgcat cgttgccgcc gcgccagacg cccatgaact tctgctgccc gccttggttg
     1501 taaatctcca gatcgaccag gcggaggttc tgcgcgctca acgactccca cttggccttg
     1561 aagtcgttga agttcgtacc gatccagagg tagtaaggat cgttgcctcc ccgccagacg
     1621 ccggcgaagg catcctgggc ttcgacgcga ctcgcgcccg cgagtagcag accgctgcac
     1681 agcagcagga tcacgattcg gctggctcgt ctcatcaagt cctcccgata aggctccatt
     1741 ttcgcacggg ccacgcgcct accgcctccc ctgcgcgatc ctcttcgcct cgggcaatgc
     1801 gcgcaaacga agcatggcca tcgtgccgag gatcgggccg ggggcgagga tggcgaaggc
     1861 ccagtgccag gtgagcgcat gctcgatgcg cggcatgatg ctgatcgacg cggtcgtgat
     1921 caggaatccg atgcatgtct gcagcgtgag agcggtgccg atgtagcgcg gatcagaaag
     1981 ctcggtgacg cacgccgaga actgcgccga gtcggccacg accgtcgcgc cccagatcgc
     2041 ggcgacagcc agcagcgcga tcggtggtcc gccgaaaagg aatccgatcg cgacgcagca
     2101 ggctgcgctg gcgatcatcg ccgccgaggc aacgatggtg cgcccgatgc gatcggcgaa
     2161 acggccggcg atcacgcagc ccaccgcgcc gcttccgatc acgatgaacg accccacttc
     2221 cgccaggcgc gccggcgagc cgctcatgat catgctcgcg cgcagcatca ccgggatcca
     2281 cgtccacatc gcgtacagct cccacatgtg gccaaagtag ccgaagttgg cgagacgcac
     2341 cgcgcggttg cggacgatgt cggccgcctg ccccaggtcg aacggctgat tgggcagcgc
     2401 gaagggtccg tcgctgacga agaacagcac gatcagcccc ccggccgccg cgagcaacga
     2461 tgcgaatgcg acattgatgc gccagctctg cgagccgacc gcgttgatga gatagggcga
     2521 cgccttgccg agcgtgagcg cgccgacgag cacgccgagc gcgaagccgc gaccttcgcg
     2581 aaaccaggtg gcgatgatct tcatccccgg tggatagacg cctgcgagaa agactccggt
     2641 caggaaacgg agcgcgatcg ccatcatcgc gtcgcgtgcg agccacgcaa acagcgcgtt
     2701 cgtaatcgct cctgccagcg ccgacaccgc gaacaaacgc cgcacgctca tcacgtccgg
     2761 caggttggcc atcgcactga tcagagtgcc agcgacgaag ccgagttgca cggccagcgt
     2821 cagccacgcg gtcgtgccgg cgctgagctt ccactccgac gcgatgcgcg gcgccacagc
     2881 ggacgccgtg aaccaaagcg acatccccag aagctcggcg atgccgacga gcagcagagc
     2941 acgcgttcgc gtcatgccgt ttgcggtcat cgatgcgcta cggcgccacc gcttcgtaga
     3001 tgcgaagtgg aagctgggtg atcgaaccga gaatctcgcg cgtcacgccc gactgacttg
     3061 ctttcgaatc acgcgcttcg acgaggacgg cgtcgacgat gtcgatgtac tttctgccgg
     3121 cgccgatcac caggttcacg accgctgctt cgagcggcga catgctcgga ttgaacgcgg
     3181 cgttctcggc aactgatccc gtgacgatgc ctccgtcgct cgtcttcagc gccacgccgg
     3241 cgtagctcgc gctgtagggc gcgtacgaac ggttcgcggc gtcgagcgcg gcgcgcgcga
     3301 cctcatcatt cgttgactca gccaacgtca tcccatgcga tcgcggcgac atcaggcccc
     3361 cggtgacgcc gaggtcaatc gggccgaagg cggcgggaag gagcgctgtc agcggagttg
     3421 ccggtgtgtc aggaaggatg atcttcagcg tcggcgccgt agtcagctcg ttgagaaact
     3481 ggcggcagta gccacacggc gccgcggaga tggccagcat ctcgacgccg gtctctccgt
     3541 tcgatagcgc ctgcacggta gccgcctgct cgccgtgaac gctgaaggtc agcgcgtgtc
     3601 cggggaactc gtagttggcg ccgaagtaaa ggctgccgct gctgccgagc gccacgacgc
     3661 cgacgaagaa gttcgagatc ggcgggatcg cgaacgtctt cgccagcgga atcagatcga
     3721 gcatgagttg ctcgatgctc atgccgccgg cgatgattct cgcaacttca cccgcgggca
     3781 gaaaccctcc cgcggcttga cgcgccgatt caatcgacgc cagttgttcc tgcgtcagca
     3841 tcccgatcct ccgtgcaatt cgcgtgaagc atgccacggg gccgctaaac tgtctgcatg
     3901 gcatcgaagc gacgcgcggt tacgttcgat ctgattcgcg acctggcgct gaagcatccc
     3961 ggcgtagaag agggaacgtc ctacggcacg cgagcgctgc gggtcaaagg aaagttcatg
     4021 gcccggctga aggaagacgg cgactcggtc gcgttccgaa tcagcttcga cgagcgcgat
     4081 ctgttgatgc aaacgaaacc gcacgccttc tacatcaccg atcactacct cggatacccc
     4141 gcagtcctga tgcgaatgtc ggccgtgacc cccgaggagg ctgcggacat cgtggcgatg
     4201 tcgtggcgca tggtggcacc gaagaagctg aagacgtagt gcaggcattc ttgcctgcaa
     4261 ccggcgggcg tcctcgcccg gcggtggtac tcctttgcga tctgaacgaa aaactaactc
     4321 ctcgactgcc cgtaataggc ccggtgcaca ggaaaatgat gcagtgggtc ctctggactc
     4381 tgatccttct atctttggca acgccggcgt tctcgcagac gttacgtgct catctccaac
     4441 acgtcttccg cgggcatgca gacgtcggcc ggcaggttgc cttcagcccc gactcgcaaa
     4501 tcctcgccac cagcagcgcc gatcgaacgg tgaagttgtg gcggctgtcc gatgacaagt
     4561 tgctgcggac gctgctgcat cccgaggggg tgacgtcgat cgcgttcagc gccgacggac
     4621 agtggctcgt cagcggaagc tacgaccaca acgtcagaat ctggcgcgtg ggcgatgggg
     4681 cgctcatgcg cacgctcaag ggacacgaag gcgtcgtctg gtccgtagcc ttcagtccgg
     4741 atggtcaacg cgtcgtcagc ggcggcgagg acaaaacggt gagagtctgg cgttgcgcgg
     4801 acggtgcgct gctaagaacc cttcgcggtc acacgctgaa tgtctggcat gtcgcgttca
     4861 gcccggatgg ctcgttgatc gcgagcggca gtttcgacaa gaccatcagg atctggcgca
     4921 gcgattcggg atcgctcgta cggacgctga cggggagcaa ggaggccgtg gtcggcatcg
     4981 acttcagccc gaatggtcag atgctcgcaa gcggcggcga cgactcatcg atccgcatct
     5041 ggcgcgtgaa ggacggagca cttctgcgca cgatcgaaac gccgaaccac gtctactccg
     5101 ttgcgttcag cgcagatggt caatggctcg ccagcggagg ccgcgagcgc ggcgcgttgg
     5161 gtacgttgtg gaagcagatc gccggtccgg gatcgacgca cggaacgact gtccggctct
     5221 ggcgcgttca cgacggaatg ctgcagcagg aactggccgg gcacgcggac gaggtcatgt
     5281 acgtcgcgct cagccgcgac ggaaagtggc tggccagcag cagtgaggac aagacggtcg
     5341 gactgtggcg gctgcaaacg tctggcagat aagcctttcc tccgtggctc cgatcctgca
     5401 attgatcagc ccacatgaag atttcctatg cccgtggtct ctctctcctc ctcctcggcc
     5461 cggccactgt cgcgcaagcg cgtgtagtgg cacggcacgt tgcgcagccg ttcacggcag
     5521 ttgcgacgaa tgacgcaacg aaaacgccgg ctctcacatc ggccagcaaa ccaggcccgg
     5581 ccatattgcg ggcgcagatc cttctcgacc gcgcgcactt ctcccccgcc gagatcgacg
     5641 gacgatacgg gagcaacatg cgttcggccg tcgcggcatt caacgcatcg cggaagattc
     5701 cgtccggctc gaacgtcagc gccgcaacgt ggaaagcgct gaacgccgac accgcaccca
     5761 cgatcacgcc ctacacgatc actgccgccg acgtgaccgg cccgtttgcg ccgatcccga
     5821 cggaaaccgc cgacaaagcg aagctcgccg cgctgggcta cacctcactc gccgaagaac
     5881 tgggtgagcg ctttcacgtc agcccggcgt tgctgcaaaa gctcaatcac ggcatcgcat
     5941 tcgacaaagc cggcgcggtc attcaggttc cgaatgtggc tcgccccccg ctgccgaaga
     6001 cggcaggaat gagcattcgc gtcagcaaga gcaggcaagc cgtcgaagcg atcgatgcga
     6061 gcggcaatgt gctggccacc tatccggcga cgatcggcag cgtccatgat cccctcccga
     6121 tcggcaagtg gaagatcaac ggcgtcgaat ggaatccgaa gttcaactac aaccccgacc
     6181 ttttctggga cgcagagccc accgacaaga aagcaaagct gcctcccggc ccgaacaacc
     6241 ctgtcggcgt cgtctggatc gatctgtcga aaccgcacta cggcattcac ggcacgcccg
     6301 atccttcgac gatcggcaag accacgtcgc acggctgcat ccgcttgacg aactgggacg
     6361 ccagcgagct cgcgaagatc gtcacaccgg gcatgcccgc ggtactggag caatgagagt
     6421 cgccaagaag atgggatggg ctgtcggcgt ggccggtgtc gccggactgg cctggatggc
     6481 gtcgggaaca acgcagagcg tgattgctcc cgcgcccgcg ttccggcagg caacgcgaac
     6541 gaatgcctcg ctgcccactg tcccggtaac gccgagaatg cttctcacac cgggcgaagg
     6601 accacgtcca ctgacgatgc cggtagaagg catcgatccg agcacgatcc acgatacgtt
     6661 cgacgagatg cgcggcggcg acacccggcg ccacgatgcg ctcgacattc tcgcggcgcg
     6721 cggcacgaac gtcgtcgcga cggacgacgg cacggtcaag aaactattca cgagcgtcgc
     6781 cggcgggttg accgtctatc aatacgatcc cgaacagcgc tactgctact actacgcgca
     6841 tctcgacgcc tacgccatgg gccttcatga aggccagatc ctgaagcgcg gcgatctcat
     6901 cggctacgtc ggcacgaccg gcaacgcgcc caaagacacc ccccatctcc acttcgccgt
     6961 gatcaagctc gatcccgatc gacgctggtg gaccggcaca gcgatcgatc cctatccgct
     7021 gttgtacagg ccgaggtaat acgacggaat taacccggcg cgcgaactgt agaatgcgag
     7081 cagttggtcc ccgtcaaatc aatgttcccg cgaaggttag cgcttgtcgc gtgcgcgctc
     7141 gctcttatcg ctagtgcgtc cgcgctctcg gcgcaggcgc cgatgttcca caacccgttc
     7201 ccgatcccga tccgtttcgg acagggtggc acaatcgaca tcgctaccgg tgacttcaac
     7261 ggtgatcaca aaccggacat cctcatcatc aatgccgcag acaatctcga ggttcttctc
     7321 aatgacggga acggctcatt cgcaccgtcc gtttcctcgc ctttcagcgg gtacttcgat
     7381 agctgggcta tcgatgatgt caatggcgac ggcaaactcg acttcgcctt ttccgacggc
     7441 attcacaccg taaaggttct cctcggcaat ggagatggaa ccttcggtgt cgaacatccg
     7501 ttcaccacgg ttcagcccgt gcacaaggtc gcactcgccg acatcaacgg cgatcaccat
     7561 ctcgacctcg tcgtcggggc agacgccagc gtcactggca tgatcggcgt ctatttcggc
     7621 gacggcacag gccagttttc caacagcggc gtcattacca actgtaactg cgatctgacg
     7681 actcttaccg ttgccgatca caacggcgac ggaaagctcg atatcactgt gggcaacgtc
     7741 tttcttgccg gcaacggcga tggttcgttt cagccgcccg tgtacctaac cgcgccgccc
     7801 ttcacaggga cgatcgccga cttcgacgcc gacggaattg ccgatcacct gtggtgcgaa
     7861 gaaggcggta gtgtcgtgtc gatccagcga ggtcgaaacg atgggagctt caccgagtct
     7921 gcactgttcg tatcggggcc gagcgcattc aagagcatag ccgcggactt cgacgatgac
     7981 ggaaaggtcg acgtggtgac gcttgattac aatcttgaaa tcccagcgct ctccttgttg
     8041 cgaggcaacg gcgacggtac ttttcaggcc cctcgcagct atcgagtggg cagaacagct
     8101 cctgaaccca attttggcca ggtacgatca ctcgcggtag cagcggacat gaacggcgat
     8161 gggaaaccgg acatcgtcgc caccccgatg catgtcaatc cccctccctt cggggaactg
     8221 gccgtgatga tcaatgacgg caccggcaag atggctgctc ccctctatac gaacctcggc
     8281 cagactgcag ggggggcgtt tgccattgga gacctcaacg gagacggaaa ggcggacgct
     8341 gttgtcgccg gcaatccggc cgaggtgctc atgggaaacg gcgatggcac gttctccgcg
     8401 cccgtacatc tcaatttcgc ggtgaacaac acacctcaac ttgtcgatct tgatggtgat
     8461 ggcaagctcg atctcttctt tgatcaggga atccctttta tcgtctacca cgggaatggc
     8521 gacggcacct tctctgccat gagcaccgta acgtttccga taggcgtagc cctctttggt
     8581 gacgttaatg gcgatacgta tcccgatata gtcacgaatg ctggcgctgg aatcgtcgtc
     8641 gccctcaacg acggacacgg cgccttctct ccgcattctc cgcttcccgg ctttgcaacc
     8701 gatcccgttg cccttgccga tttcaatggc gatggcaaac tcgacatcct ttcaactgga
     8761 acgagtgttc gtttgggaaa cggcgacggg agttttggcg cgccgatcga tttcccggtc
     8821 tcaccgacgc cgttcgggcc ggtcaggacc gctgatttcg accgcgacgg gaagatggac
     8881 gtggccttcg gaaccaccgt gttgtttggc gatggaagcg gccgcttctc gcgctacgcc
     8941 cgttttcgga cgacttgggt aaccgcactc gatgtcgctg atattgacgg caacggatcg
     9001 cccgacctcg ttgtaaccaa ttcgattgcg gacgatgttg acattcttct gacctacgga
     9061 tctccggcgc ccgtggcaca gccaggcctt gccctcacat ctgataagcc ctcgccgcag
     9121 tacggcgaag ccgtcaccat cacggccaca atgagcggcg gaacgacccc actcacagga
     9181 gccgtgacct tttcaatcga tggtattccg gtggctatga gcattctcga cccgaccgga
     9241 atagcgacgt tccgatccgg gtaccgggtc ggttctttcg tcgtgacggc aacatacagc
     9301 ggggacgaga actatttgag tgccaccaat tccttgaatc tgaacgtgag taaagcgctc
     9361 acaagcatca gtgtctccgc gttcccgaac ccggcaagac tccatgacgg cgtacgcatt
     9421 gtcgcaagca tgtcgtatcc agtgccctcg gctgttccag gaccctcggg ctcgctcatt
     9481 ctgcgcgaag gcacgacgac cctcaagacc agctcttttt ccatgaacac tatagtcagc
     9541 acgttgcctt tgggcactca cacattgacc gtggattatg ccggcgatgc gaactaccaa
     9601 gcttcaacgg cgagctatga gcaggtcatc atcaaaccac tcctgttcat gtcgatttcc
     9661 acatcgccca gtcagccaat cgctggcacc gacgtgaccg tccgggcatc gtgtacggtg
     9721 aatgctaccg gtacaatctc tttctggatc aacggcgctg cggcaggatc ggcgccgatc
     9781 gtccagggca ttgctgagat caccgccacg atgaagttag ggctgaatac ggtccacgtc
     9841 acctatccgg gcgatggcac gtacgacagt tcgactgctg accagaacat caccgggtat
     9901 atcagccaga ccggaacccc cgtcatcgcc gcgaaaggct ccgcgttcct cggacagggc
     9961 gacatcagcg ttcagtggtc gcaagtcgtg aacgcgacct cttacactat ttaccggcgc
    10021 acctcaattg gcagtggatg ggaagcgtac aagacgttct cgggcaacac ttccggatat
    10081 tccgacacca tgccgatcaa tacctgctgg ctgatggcga tcacagcgca aacgccttcg
    10141 ggcacaaccc cgatgagcgc tccggacctc gttacatcag cacagttcag caacgtcgtc
    10201 gcaccaggca cggcgatcaa ggcgctggat ctgacggaga cacgtaccgc cgtcaacctc
    10261 gttcgcgcgt tcgcaaactt gcccgccgtc gccttcacga acaccgtggc gcccggtgaa
    10321 cgtatcgccg cactcgacgt catgcagttg cgtagcgggc tttccgaagc ccgcgcggcc
    10381 atcggccttc cgctcggctt cagcgacccg cagttagctc cgggcacatc gatcattcgt
    10441 gccgcccacg tgctggagct ccgcgccggc gtcgattgac gccatcaggc tcaacatttg
    10501 ctaccccgcg ctgcgtcgaa aggggtttgc gatgcagaga agctgtgtgg tgattcttgt
    10561 cttgctcttg atgaccgttc cgatgttcgc agaagacgcg ccagcgccgc cgccgaaacc
    10621 atggacgagc agcatcggcg ctggcctggc aatcaccagc ggcaacaccg acacaaagaa
    10681 ctacaacctc gccttcgcca cgaaatacga tccgaagacg cggctcgtct tcaaggctga
    10741 cgcgctctac ttgcggggcg actcgggcgg cgtgacgcag gtggacaagg cgtcggctga
    10801 tgcccgcgag gagtacgcgc tctccgaccg cacgttcgcg ttcgccgagg tgtcgtacct
    10861 gcgcgatccg ttcaaggaca tcaattactt cgtcgcgccg ctcgccggtg gcggctatcg
    10921 catcatcaag agcgacgctc gcaacctcac cgtcgatggc gccctcggcg cagaattcga
    10981 gagcaacgca ggcgccgggc gaacgagcgg cggcgcggcg aaggccggcg aaaacttcga
    11041 ttggacgctg tcgccaacga gcaagatcac gcagaagctc acagcgatct ggaagacgag
    11101 cgatttcggc gacgccctct accacttcga cgccggcctc acgaccacgg tggccacgcg
    11161 cctggagctg aagctggcct gggtctacga ctacaaaacc aagccgccct cgtccagcgt
    11221 gaaaaagggc gacagcgcgc tcttcgccgc gctgctgttc aagttctgac ggaatggggg
    11281 tcagaccttt gatcgtcgca gcaccgaatc ggtgaggtga aagtggttcg tcatcagcac
    11341 ccaggctgtg acggaccaac cgaatcgcaa cgtcgcctca ccgaggaagg tcaggaacgt
    11401 ttccctgtcc cggttggtgc gaaagatcgc gcggcgttca ttgcctcggg aagtgatgtg
    11461 gtacagcgca tcagcgaatt caagtcgtaa cggtcgtgcc atagtgccac cgaggactga
    11521 agtccaacag gaacttttcc gagcctgatg cgcgcggcct ccacgggaac cctctccgca
    11581 tctccgcgcc tccgcgtgaa acggttttcg acgaaagcgc cccgctataa tccccctccc
    11641 cgatgaagcc cacgcgcgcg caactggagg acgttctcgc ctccctcgat ggttccgggt
    11701 tgcgctatgc agacattcgc tggactgcca cgcatgagca gcatgtgcgt gttcgcaacg
    11761 gagaggtgga gacgctggct tcgaccatcg accgggccat cggcgttcgc gtgctcgtcg
    11821 gcaacgggtg gggattcgcg gcgaacagcg acacttccga ggagtcgctg aggaagacgg
    11881 cgaaacgggc gctggaggtg gcggcggcga gcaacatcgc ctcgacgcag cgggtgacgc
    11941 tgagtgacat cgagccgcac gtggcggcgt ggtcctcgac gtacgagatc gatccctggt
    12001 cgattccgct ggagaagaag atcgagcatc tgctccagtc gaccgagccg atgcgcggcg
    12061 atgcccgcat tcatcaggta tccggcgaca tctcctgcta ccgccaggaa aagatcttcg
    12121 cgtcgaccgt cgggagtttc atcgagcaga ccacgacgga gatgggcggc ggggtcgagg
    12181 ctgtagccat cgagggtgat gagttccagc gccgcacgta tcccaatccg ttcggcggcg
    12241 actaccaggc cgcgggatgg gagttcatcg agcagctcaa cctgcccggc aaggcgctgg
    12301 agatccgcga tgaggcgctg gcgctgctca tcgcgccaaa agcgccggcg gggcgcttcg
    12361 atctcatcgt cggatcggcg cagcttgcgc ttcaggtcca cgagtcgtgc ggccacccca
    12421 ccgagctcga tcgggccatg ggcctggaga tctcgctcgc cggcggatcg ttcctgcagc
    12481 cgtcgatgct cggcaacttc cgctacggat ccgatctcgt caacatcgtc ggcgacgcca
    12541 cgatccccgg ctcgatcggc tcattcggct tcgatgacga cggcgttccc gcgcaacgct
    12601 ttcacctgat cgagaagggc atgttcgtcg gatacctgac cggacgcgac actgcgccga
    12661 cgatcggtcg cgcgtcgaac ggcaccgttc gggcggaaac cgcggcacgc attccgatca
    12721 tccgcatgac gaacatcaat ctcgagccgg ggacgacgcc gatggcggac atcatcgccg
    12781 acacgaagcg cggcatcttc gtcgagacga acaagtcgtg gtcgatcgac gatctccgcc
    12841 tcaactttca attcggctgc gaggtggcct gggagatcga gaacggccgc ctcgggcggc
    12901 tgctgcgcaa tccgctctac accggcacga cacccgactt ctggcgcagc tgcgacgccg
    12961 tcgccgaccg cgccagctgg cagatctggg gcctcccgaa ctgcggaaaa ggcgatccga
    13021 tgcagacgat gcgcgtggcg cacggagcgc ccgcggcgag atttcgcaac gtggagatgg
    13081 ggtgagtttg aaaggatgaa ggcagaagga cgaaggatga actgaccgat tgaacggttc
    13141 atccttcatc tttcatcctt caattgtcat gcgcaacgaa cacgactcat tcgccatcct
    13201 cgatcaggcg ctggccgctg ctggcacggc cgaggcggat gctgtgttca tttcgaccga
    13261 ccagaacatc agcaggttcg ccaactccaa cgttcaccaa aacatgtcgg agatcagcgc
    13321 ggagctgtcg ttgcgtgtga tcgtcgacaa cgccatgggc gtggcatcga cgactgtatt
    13381 tgatgatgct gagatcgagc gcacggccgc gctggcgcgc gaggcggcac gtcacgccga
    13441 tccgctgccg aacttcagcg ggctttaccg cgcgaatgag ccactgccct cggtgcgcac
    13501 attcgatgac gccaccgcga acatcgcccc tgcggagaaa gcgcgcgcac ttcgcacgat
    13561 gttcgaccgc ggcgttgcaa acggggtcca tttcgccggc gtctacggca ccggcgcctc
    13621 gtcggtcgcc gtcgccaaca cccacggcgt ccggcgctat tgcacgatga ccgcgtccga
    13681 cgccacagtc atcgccatcg gcgcgaacgg ctccggctac gccacggccg tcgaccgctc
    13741 gaacgtcgac gtcaccgccc tcggcgaaga ggccgcgttc aaggccgccc ttcgcgctga
    13801 cgtcatcgaa gacatcgcac ccggcccgta cgacgtcatc ctcgaaccgc ccgcgaccgc
    13861 cgaggtgctc gagtggatga cgatgatcgc gttcagcggg tcggcgtacg aggatggctc
    13921 gtcattcttc gtcggcaaca tcggcaacca ggtcctcggc gcgaatttca cattgactga
    13981 cgatgcgctc gacaacgcgt tcctcccctt cccgttcgac atcgaaggac tgcccaagcg
    14041 gcgcgttgcc atcatcgagc gcggcatcgt ccgcacgccg gtcgtcgaca aggcctacgc
    14101 cgaccgtctc ggatttgcgc ccaccgccaa cggttggagc ctcggcgggt ccgatcacgg
    14161 ctccgcgctt cacctctcca tcgaaggcgg cgatgccacg cgcgaggagc tgatccgctc
    14221 gacgaaacgc ggcatctggg tcacccgctt caactacgtc aacggcctcc tcgagccgcg
    14281 aaccgcgctc atgaccggca ccacccgcga cggcacgttc ctcatccgcg atggcgaaat
    14341 cgtggctcgc ctgccgaatc tccgctggac gcagtcgatc gtcgaggctt tctcgcgcat
    14401 cgaaggactg acgcgcgagc gccgccgcgt cggcacctgg tacaaccact tcggcggtac
    14461 gatcgcgccg gtgatgaaga tcgccggttg gaactttacg gggaaacagc agacaggcgg
    14521 catggcacaa agctcgctcg gggattgaga aaggatgaag gcagaagaat gagggatgag
    14581 ccgggccgct gtggcgttca tccttcatcc ttccgcccgc atccgtcgta attcatgtct
    14641 caatacatcc ttcgccgatt cctgcagatg attccgatcg cgttcgggat cctgacgctc
    14701 gtcttttcgc tgatccacat gatcccgggc gatccggcgg ttcagatcgc aggcgaagga
    14761 gcgcgccccg aggatgtcgt ggccatccgc aaagcgctgg gactcgatca gccgctctgg
    14821 acgcagtacg tccattacgt cacgcacgtc atccgcggcg acctcggcac ctcgttccag
    14881 accaatacca gcgtcaccga acagatcaag acacgctacc cggccacgat cgagctggcg
    14941 ttcggctcga tgttcgtcgc acttctagtc gccttccccc tcgggatcat ttccgcgatc
    15001 tatcgaaact catggatcga caatctggcg cggttcttcg cgttgatcgg tgtttcgatg
    15061 ccgagcttct ggttcggacc gctgctcatc atcgccttcg ccatcaatca cgagtggttc
    15121 cccgtctccg gccgcgacgc cggattgcgg tcgttggtcc tgccgtcgct gacgatgggc
    15181 ctcgcgctct ccgcgatcct gacgcgcatg atccgcattt ccctcgctga cgaattgacg
    15241 cagctctacg tcaccacggc cgtcgccaaa ggcgtcacgc gagcacaagc cattttccgg
    15301 cacgcgctga aaaacgcgct gattccggtg atcacagtcc tggcgctgca gttcggttca
    15361 ctgctgaccg gtgcgatcat caccgagcaa atcttctcgt ggccgggtct gggacgcctt
    15421 cttctcacct caatctcaac gcgcgactac ccgcaggtgc aggcctcaat tctcgtcatc
    15481 gcgctgacgt acatcctcgt caatttcgcc agcgacctgc tgtacggcgt ggtcgacccg
    15541 aggatcaaac ttgagtgagg gtgtcggttg tcgggggtcg ggggtcggag ctgcccgaat
    15601 cactcacctc tgactctcga agacctgaga actaacatcc agcatccgat atccgacgct
    15661 cgacacccga cacccgacaa accatgaagc gctttttcgg taacttcgca ttcacctcag
    15721 ggttgatcct gaccgtggcg ctgatcgtcg tcgcgctcgc ggcgccgctg atcgcgcctt
    15781 tcgatccgaa tcagcaggac acgggacggc gcctcgaagc gccttcgcac gaacatctcc
    15841 tcggtctcga cgacctcggg cgcgatgtct tgtcgcgcat catctggggc tctcgcgttt
    15901 cgctgcgcgt cgggttcagc gtcgtcatcc tggcttcgat catcggcatc acgctcggag
    15961 caatggccgg ttacttcgga ggttggatgg agaccgtcat catgcgcgtc accgacatcc
    16021 tcctctcctt ccccggcatc ctcctggcga tcgcgatggt ggcggtgctc ggcccgagtc
    16081 tcaacaacgt tgtcatcgcg ctggcaacgc agggatgggt cggctacgcg aggcttggac
    16141 gcggccaagt gctcaaggtg cgggagatgg attacgtcgt cgcagcgaag gctctcggag
    16201 cccggtcgcc gcgcgtaatc ctgcggcacg tcttgccgaa catcgtcaat cctgtgatcg
    16261 tgatggccac gctcggcctg gccggcgcga tcttgtccga ggccgcgctg tcgtttctgg
    16321 gactcggggt gcaaccgcca acgccgtcct ggggagcaat gttgacctcc ggacgacgat
    16381 acctcgggct cgccaatcac ctcgccatct ggccgggtgt cgccatcatg ctggccgtga
    16441 tgggcctcaa cttcctcggc gatgggctta tcgatgcgct cgatccgaag tatcggaaag
    16501 agatgtaatg cctacttctt catgaacacg acgaccgcgc gcgccggatc atcgcggaag
    16561 gtgcgcaccg gcgtgtagcc gagcgcgcgc aacgcttcga cgcgcgatgg aacatcgaga
    16621 tccgtctcgt acaacgccac agcgtcggcg gtcacctcgg gcaacttctc cggcggggta
    16681 ccgaggtccg acatggccat cttgtcgccc aggaagagtt tgtcgccgaa cgcccagagc
    16741 tgggaggcgg cgatggaatg cacgcgcgga ttcgcaccca gccattgcgc cgcttcgacg
    16801 gcaggctgcg acttgcgcgc gaaatagcgc attccgtgca aatcccaaat gagcgaggtc
    16861 gcgaccagcg cgattgcgat gcggcggtga cttcggtaca tcgacgcgaa gccgatcgca
    16921 gcggcgatcg ctaggaaagg aatcatcacc tgcaggtagc gaagctcctt gtgtctgacc
    16981 gcggagaatg ccagcagcgg aatgatgacg aaccagatcg cacggctgcg gcgcgcgtac
    17041 caaagcaacg gaagaagcag cggtgagcac catcgcacga gattcgcgat gtaccaccac
    17101 ggcgcctgat acttcacccg cgacgcaaag tcgggagcga cgagtgtgag gtgggtgaac
    17161 ttgatgaagc tcgagaatgg tgcgccccag gtgagccagt cgtagaggcc gaccgtcaag
    17221 ctgatcgcaa ctgtcgctcc gaccatcacc ttcagcgcct gcatccacgg cacagacaag
    17281 agtgtctgtg ccacaccatt ccgcgcatgg attggacatt ctggcgaggg gacagtctgg
    17341 acagaacact cttgcgggct gccagtttga gcagacaact cttgcgagcg gccagaatga
    17401 gcagacccca cttgcgaggg ttcaatgtgg cacagacact cttgtctgtg ctttgtgatc
    17461 atcaccagga gcggaacaag aaatacgatc tcactgaagc ggtcggcgaa tgcgagtccg
    17521 gccaacgccc ccgccgcaaa cggccatcgc tcgacgatca gcgccgctcc cacaacgcac
    17581 gcagccgcca gcgttcgcgg gtaaaccgtg ctgccgaagc cgaggggaat ccagtgcatg
    17641 gcaaaaagga gtgaggccac gagcgcggcc cgctcgtcct cgcaccagcg cagcgccagc
    17701 cggtagacga gaacgatcgt gagagctgtc agcgcgatga aaggcagcgc cgacgcaagg
    17761 atcagcgtcc gcgtgtcgct gatcccgagc ctcgatgcga tccagacgac cggcgcgacg
    17821 accacgtccg gcacgaagag attgcggata tcccacggat gaaagtcgag tccgcgcgct
    17881 cgccggaagg cctcggacaa cacctccacg tcgtctccgg tcatgaagcc gaagtagcga
    17941 tgcgcgagcc agagctgcgc gatcgcgacc gcggcgatga tcgcggccag atgcttacgc
    18001 atcgccgatc atcgccagaa actcgtcctc ggtcaggacc ggcacgccaa gcgatttcgc
    18061 tttgtcgagc ttcgagcccg cggcgtcgcc ggcgatcacg tacgacgtct tcgccgacac
    18121 ggagccggac ggtttgcccc cttcacgctc gatcagtttg tgaatctcgt cgcgcgagaa
    18181 acgagcgagc gtgccggtga cgacgagcgt cttgcctgcg aggagatcgc cgcgcacctc
    18241 ggcgacatgc gtcggtgcca caccgagtcg ctgcatcttc tcgatgcgct cgcggtttgc
    18301 gggaaccgaa aaatagaagg tgatcgactc cgccactttc ggcccgatct cgtccacgtc
    18361 gaccaattcg tcgaccgttg cattcatgag cgcgtcgagc gaaccgaagc gcgcggcgag
    18421 caatttagcc gcgcgctcgc cgaccatgcg tataccgatc gcgaagatca cacgcgacag
    18481 ttccgccgtc ttcgacttct cgatcccctc ggccagtttg cgcgcggaca gctcgccttt
    18541 gcccggcagc gccgcaacgt cctcctcgcg aagttcgtaa agcgatgcgt aatcggtgat
    18601 gagcttcgcg tcgagcagtt gcgtgatcgt ctggtagccg agtccttcga tgtccatcgc
    18661 tttgcgcgcg ccgaaatgaa agagggactc gcgcacgatg gccgggcacc cctggttgat
    18721 gcagcggatg gccacctcgc cttcgaaacg atgcagcggc tcgccgcagg cgggacacgt
    18781 ttcgggcaac gccaccggca ctgttccctc ggggcgttta tcgacaagga catcgacgac
    18841 tttcgggatg atgtcgccgc ccttctcgac gacgatggaa tcgccgacgc ggatgtcttt
    18901 gcgccccacc tcgtcgaagt tgtgcaacga cgcgttcacg accttcgtgc cgccgatgtg
    18961 cacgggatcg aattcggcga ccggcgtgac cgcaccggtt cgcccgacgt acaggttgat
    19021 cgcgcgtacg atcgtctgcg cggcctcggg tggatacttg aacgcaatcg cccagcgtgg
    19081 cgccttcgac gtcgcgccca gctcctgttg ctgcgcgcgc ttgttgacct tgatcacgat
    19141 gccgtcgatg tcgaaactga ggtcgtgccg ctgctcgcgc accgtgtcga tgaaggcctc
    19201 gaccgcggcc atgcccgcga agacatcccg cttcggattg agcgggaatc ccatcgcggc
    19261 cagaatctcg tacgcctgtg cctgcgaggt aacgcgaacg tcatcggttg cgaccaagtg
    19321 atagacgaac gcgctgagcc gccgctgcgc gaccagcttc ggatccttct ggcgcagcga
    19381 tccggccgcg gcgttgcgcg gattggcgag aacaggttgc cccgcttcct ccagttgcgc
    19441 attgagcaac gcaaagtcgt tcgacgaaat gtagatctcg ccgcgaacct cgacgcgctt
    19501 cagcttcgtg gcgatcttca acggcaggcc gcgcaccgtg cgcacattca cggtcacgtc
    19561 gtcgccgcgt acgccgtcgc cacgcgtcgc gccccgcgcg aatgcgccgt tctcgtacag
    19621 cagatcgatc gagaccccgt cgatcttcag ctccgcctcg tactcgacct cctcgacgcc
    19681 gagtcccttc ttcacgcggg catcccactc gtgcagctct tcgagcgtgt aggcgttctc
    19741 gatcgacagc atcggcgggt cgtgaatgac cgtggcgaac tcgccggtca tcggagcgcc
    19801 gcccacgcgc tgcgagggcg agttcggatc gaagagctcg ggatgctgcg cctccagctc
    19861 ctgaagcctg cgaagacgct gatcgaattc gaagtcgctg atctccggcg tggcgtaaac
    19921 gtagtaaagc tggctgtgcc gctccagatc ggcgcgcagt gtctcgattt ccttcttcgg
    19981 cgtcatagcg cgcgaagttt acgatgccaa ggccgcgcgc tactcgtgtt cgtgaatctg
    20041 ctgggccagg tagttcggcg cgccgacctg ttcgatcaga gagagctgcg cttccagcca
    20101 gtcgatatgc tcttcctcgt cttcaaggat ttcttccagc agatgacggc tgttgttgtc
    20161 ctctgcttcg cgacaggtct cgattccggc gttgagacgc ttgacggagt cgacctcgag
    20221 ggccagatcg atgctcaact gttccggtac cgtctcgccg atcgtgacct tgaacaggcg
    20281 ctgcatgttc gggatgcctt ctagaaagag cacacgctcg atcaggctct cggcatgctt
    20341 catctcaccg atggagtgct tgcgaatgcg atcgtgaagg tgctggtaac cccagttgtc
    20401 gcacatctcc gcgtgaacga agtactggtt gatggcggtg agctcggacg tcagcacgtc
    20461 gttgaggatg tcgatgatct tctggtcgcc cttcatttcg aaaatctccc ccgtcactct
    20521 atcaagccac gctctccgac cacacttccg tggacggcag cggcacgaag gcgaccggtg
    20581 acacgctttc gttcgcgctc aacatcttgc ggaggaagtt atggcagccg ccgcagtcgg
    20641 taccgattcc acactcctgc agatcggcaa gcgtttccga tccaccttcg atggctcgca
    20701 gcacgtcacg ttcgtttttc gctcggcaga ggcagacgat cattcgattt catccgatca
    20761 attgagatta cgtctcaata aggtgaacac acgatcgcct attccgggcg cggtcactgt
    20821 gtcaacggtc acatccgcct gtgatcacgc tggccgggtg agataccgcg aacgctcgac
    20881 ggtcttctcc gcaaagccca tcgaccgcag ccgctcgtcg acggcagcca ggaagttgcg
    20941 ccggcccttg atgaggttga gattgagcgc cacgaaccgc aatcctcggg ggctgtccca
    21001 atagacgatc agccggtcgc cacccagcat ctgttcggtt ccgcggagcg catcccaccg
    21061 gaccattcgc gatcggaact gatggagcgg gcgaagggtc agcgtctcgt cgccgatctc
    21121 cgcgatcggc cgccgcgcgc caagccacac tttcaatccg ccgatccaaa gcgtggcgat
    21181 gtacgcccag accgcgtgct caccaatcca accgtagcgg gctgagggac tcacgaacaa
    21241 cgccaccagg attgccgccg tgacggcagc agctacgagc acgatcgaaa aaccggcccg
    21301 cgccacccac gggcggaacg gataaacctc agttcggtcg ggcattgtgc aacgaagccg
    21361 gatgccgtaa tctcatgcag cgtctaagct tgaggccggt atgcgtttcg cataataagg
    21421 tagcagaggg agttttggtg ctcatggtgg gaaagctcga cgggaccggc cggaggatcc
    21481 tggtcatcga tgatgacttg gcaatccgtg tcctgctgca ggccgttttg aagcggatga
    21541 agttcgaagt cgagcttgcc gaggatggcg ccgtcggtct ggaaaagctc aagaacgatc
    21601 ccctgtacga cctcattctc ctcgatctca tgatgccgcg tgtcaatggg tacgagttca
    21661 tcgagcgagt ccgccgggag caaaaggatg cgccgcctca catccttgtc ttcactgccg
    21721 ccggcaagcg aggcgtcgac aagattcctc cgggcgccat ctgcaactcc atcctcaagc
    21781 cgttcgacct cgaaaaattc atcgacatga tcgcggaatg cctcgatggt ccccacaagc
    21841 ctcacctggc cgcgcctctc tcctgagcca gcttccattc catctgtcga atgatgccga
    21901 ggaggatcgt gacctctcgg gcgtcgagat cggcgcgtgc ggcgatgagg cgcaggtcgt
    21961 cgtagatgtg gtccgggttg tcgccgagta gatacccgac ctcggccagg aaagcctgga
    22021 cgcgttcgtg aagccgctcg atcatcacgg ccggcgccag gtcgcggggt gtggcggcgc
    22081 gcgacgtggt cgacaattcg tagcagaaga cgccgacgga ttgcgcgagg ttcatcgtcg
    22141 gaaacgcagg gtccgtcggg atgatggccg tgcgctgaca gtgccggacc tcttcgctgg
    22201 tgaggccgct gttctcgcgt ccgaagacga gagccagcgt ctgcgagtca tccatggatg
    22261 aaaaaagttg cgcgatgccg acgggctgca aatccgaggg tgtggagcga ccgcgggaag
    22321 acgtggtggc gatggtgaca tgcgcatcgg cgag
//
LOCUS       DATLVW010000549         9883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_477204, whole genome shotgun sequence.
ACCESSION   DATLVW010000549 DATLVW010000000
VERSION     DATLVW010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477204"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VNN25_16380"
     CDS             <1..455
                     /locus_tag="VNN25_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXH93161.1"
                     /translation="RAGWVRLKSADPRDRPDINFRYFDEGTPNDPSDVNACVNGVEFV
                     RTISKHNTAIKQEITPGPNVQGSDQIAQYVKDNAWGHHASCTNRMGKADDPNAVVDSE
                     FRVIGTQNLRIVDASIFPRIPGFFIVTPVYMIAEKASDAILATAKGGD"
     gene            567..722
                     /locus_tag="VNN25_16385"
     CDS             567..722
                     /locus_tag="VNN25_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH93162.1"
                     /translation="MADIPDLEACSAFGATPQEALTEAVRAKEGWLAAARDAGKPIPE
                     PQYRPLI"
     gene            complement(882..1880)
                     /locus_tag="VNN25_16390"
     CDS             complement(882..1880)
                     /locus_tag="VNN25_16390"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HXH93163.1"
                     /translation="MSATPAPIPPPEDPNLDLGFGSVVARESRKRFLNRDGTFNVRRE
                     GLGSWEALSFYHYFLTISWPRFLAWVSGAYVVLNALFALAFIACGPEALTGFSNQSMG
                     ERFTRSFFFSVHTLATIGYGNIVPVTLAANVLVAFESLVGLLGFAIIAGIMFSRFARP
                     RAEIIFSRTAVVAPYRDGKALMFRIVNKKRNEIVEMEAKVLLARRKRGGSSVTDREFL
                     PLKLERDRVVFFPLSWTIVHPIDDSSPLRGCAATELREMDAELLILLNGFDETFSQTV
                     HTRSSYKVDEVVWGAKFTSMFNPLDDDGIISIDIRKLHEIERTEPAQTAVSSKAVS"
     gene            complement(1945..2562)
                     /locus_tag="VNN25_16395"
     CDS             complement(1945..2562)
                     /locus_tag="VNN25_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93164.1"
                     /translation="MQELKAEKPPESPAGSDPTSRLMARLEEEGFTTDLTDDDWRNYG
                     EIIFRRAEKIAFASGETIFIFTRVRDLNERILHQTSESVVNAYKARSVGGKALSVLQS
                     TTVYHCLVAEGDQPHNDLLTGYVTRAGGATFIPVIIVPEINQVIYPNLEEKVASVRPR
                     IEYLQYLLGERLETVDMHRPTVHAMWISLALVVVLLLAIAVAAIR"
     gene            complement(2543..2902)
                     /locus_tag="VNN25_16400"
     CDS             complement(2543..2902)
                     /locus_tag="VNN25_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93165.1"
                     /translation="MILVVEDDPQVARLVTLVLQRNGRECQVVSDGSSALVRANELRP
                     QMILADLTIKGMNGAALCSALKAQDSTRSIPYIVVSGDRDIAEKARQCGADDYLVKPF
                     EFPDLIRLVEKYARAES"
     gene            complement(2915..3391)
                     /locus_tag="VNN25_16405"
     CDS             complement(2915..3391)
                     /locus_tag="VNN25_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93166.1"
                     /translation="MGKSTIRVVTVPRSVTVILLIILSAAMSLLILSLSGRAYLGARL
                     TMSELVSLIHRYDAGAVSNDALLATAAPAIADLLFFIPWGALAFLAFDGGDEHRLRTY
                     LVTLAVGVTFALGLMAWQSRLPTRVTGWDDAGWNALGCLFGAVAGHLRKRLRIRFD"
     gene            3671..4522
                     /locus_tag="VNN25_16410"
     CDS             3671..4522
                     /locus_tag="VNN25_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93167.1"
                     /translation="MKKALVFVFAAALATSAFAQGNSDPELNADHGNSQAHVSNFAGG
                     GGSGNLIYHTGGKVILNAHVVEIFWGSFPSGYTTNMQNFRNQFGTTGEYNVITQYTGI
                     DDATGTTGAINLTNLAGGPIDYFDTTTPPTNCTDAVVQAEVKKVIAAQGSTDYSAIYE
                     VFIPSGSYSSSGSSTSCGGPNLAYCAYHSHYVDGAGKNVKYSIEPWAGCSGCQAFGNT
                     STDQEHFVCHETREAVTDALGNAWFDRRGNEADDKCAWTPTPFISGGYGYQYEWSNAN
                     GGCVQTR"
     gene            complement(4945..5367)
                     /locus_tag="VNN25_16415"
     CDS             complement(4945..5367)
                     /locus_tag="VNN25_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93168.1"
                     /translation="MHRKLILATIAAAAAACATTPAPAPASAPASTAAATAATPPAAA
                     PAAALADHEGDSPETAVTVPANAPNEGIDFQNNWIYDRYGRFRRLRGGIAHAGEGSAM
                     RRYEVVTFEVPDHTEHTVYFDITENEKTWSNTPAPKPH"
     gene            complement(5376..6431)
                     /gene="rsgA"
                     /locus_tag="VNN25_16420"
     CDS             complement(5376..6431)
                     /gene="rsgA"
                     /locus_tag="VNN25_16420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXH93169.1"
                     /translation="MGRRGSRGRGTEDWEEPVGDRRGGKRRKPDAADDPADDGAWGEA
                     GDADPRTAAMVISVGSGRCRLFYDGREFDCVVPTELAVRQKSALAVGDRVVTEALADG
                     TCRLAAVLARRTRLSRPDPLNPHLQRLIAANIDIVIHVVSVKAPPLRPRLIDRFLIAI
                     QRGGAEAAICVNKIDLLEPDERARELASLDPYRALDVPVVGCSIKSGEGIAELRALVE
                     GRTCALVGHSGVGKSSILNALDERLDIATGELHKRGTGRHTTTASTLHDLGGNTFLID
                     TPGIREFGLWKLTLETLRDYFPEFSEPGEYCRFNDCSHLPEPGCAVKERAEAGELSNA
                     RYQAYVRLAEDLSSPKG"
     gene            complement(6621..8285)
                     /locus_tag="VNN25_16425"
     CDS             complement(6621..8285)
                     /locus_tag="VNN25_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXH93170.1"
                     /translation="MKKLALLFLAAVSLNAATPVDPSQVAPDIDKRLARFQKVEMPFS
                     FAGMSAREKRVVDEMIAACRDLEDIYWRQNDPDDIAVYNSLAHATDPKLQAARRYLWL
                     NGSHYDLLNHNEPFIGGEPMPPGRHLLPKGVSRDEIEAYVAAHPAEKKAIYDEHSVVE
                     ATNRTPLKLKATPYHVKYRHWLEAAAGHLRNAAAASDDKAFANYLRMRAKALLTDDYY
                     ASDLAWVRLKDPKFDLIFAPYETYLDDLLGVKTSYGASVLVRNESESKKLAVFQKYVP
                     DIQDALPLAAEDRPSKKGLASPMEVMDAPFRAGDLRHGYQAAADNLPNDPRIHEKVGS
                     KMIFFKNFTDARVNYVILPLAKYIMRVDQAAQASGEGYLAAVMMHEISHGLGPAFARK
                     GGQHIDIREAIGPAYSGLEEAKADVTGMFGLKWLVDRGALPKERLEEYYASYVAGIFR
                     TVRFGTAEAHGRAEMMEFNYLAEKGAIVREASGRYGIDYAKMPDALASLAKELLDIEA
                     TGDRARAESWFNRYDKMPAELRAALDAATNVPVDIDPLVPFHEGVQ"
     gene            complement(8397..>9883)
                     /locus_tag="VNN25_16430"
     CDS             complement(8397..>9883)
                     /locus_tag="VNN25_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH93171.1"
                     /translation="PEDRQRLMSRLKEQRVLSNIEVRLRKRDGTPIWVLENINLLDGD
                     VIEGTIIDITDRKTAQEQMEYQAYHDILTGLPNRLLFRDRIEIALAHAKRHRTNAAVM
                     FLDLDQFKLVNDTLGHTVGDGLLQEVANRLVATIRADDTVARMGGDEFTVLLTDIKET
                     GSSAIVAQKLLDAISRPMVIEGHEIYATTSIGVALFPDDGGDAETLLRRSDSAMYRAK
                     EAGRNNFQFAPVTTIEMTSERLEIERSLHHAFEREEFVVHYQPMTNLLTGNIVGAEAL
                     IRWNHPEKGLMSPDDFIPIAEECGLILPIGEWVLRTAVRQMKEWHPEHGLLRVAVNLS
                     ARQFQQRDLTAMIERILTESDYPPELLDIEITESTAMHNADVSLAVMNRLRSMGVRIS
                     IDDFGTGYSSLSYLKRFPIDTVKIDQNFVRDLSDVSNDGAIITAVISMARALNLRVVA
                     EGVETEAQLDFLRRENCEVVQGFLHSRPVSAAEFDRSLRARTVR"
BASE COUNT         1930 a         3058 c         3022 g         1873 t
ORIGIN      
        1 accgggcagg ttgggtgcga ctcaagtcgg ccgatccgcg cgacagaccc gacatcaact
       61 tccgctactt cgacgaggga acaccgaacg atccgtccga cgtgaacgca tgcgtcaacg
      121 gcgtggagtt cgtccgaacg atctcgaagc acaacacggc gatcaaacag gagatcacgc
      181 ccggacccaa tgtgcagggc tccgatcaga tcgcgcaata cgtcaaagac aatgcctggg
      241 gccaccacgc ttcgtgcacg aatcggatgg gaaaggcgga cgatccgaac gccgtggtcg
      301 acagcgagtt ccgcgtcatc ggcacgcaga acctccgcat cgtcgacgca tcaatcttcc
      361 cgcgcatccc cggtttcttc atcgtcaccc cggtctacat gattgccgag aaagccagcg
      421 acgccattct cgccaccgca aagggcgggg actgatgaag cggatcgcat tttgcgtcta
      481 atcgagcgtt acaagcttcg gttgaaggag gactgatgcg cgattatcac atcaacgtgt
      541 ttttcagcga cgaagatggt ggatgaatcg cggacattcc cgacctcgaa gcctgctccg
      601 cgttcggtgc cactccgcag gaagccctga ccgaggccgt ccgcgcaaaa gaaggatggc
      661 tcgccgctgc gcgtgacgcg gggaaaccaa ttcccgagcc gcagtaccgc ccgctcatct
      721 gactccgcca tcaaaacatt tgatccagcc atcaaaagac ttgactcggc catcaagaga
      781 tttgatcccg ccatcaagtg atttgactcg gccatcaaaa catttgactc ggtcatcaaa
      841 tgatttgact cggtcatcaa aaccgatggt ccggaaggtc gttaactgac cgctttcgat
      901 gacaccgccg tctgggccgg ctccgtgcgc tcgatctcgt ggagcttgcg gatgtcgatg
      961 gagatgattc cgtcgtcgtc cagcgggttg aacatgctcg tgaacttcgc cccccagact
     1021 acttcgtcga ctttgtagga cgagcgcgtg tgaacggtct gcgagaaggt ctcgtcgaag
     1081 ccgttgagaa ggatgagcaa ctcggcatcc atctctcgca gttcggtggc agcgcagccg
     1141 cgcagcgggc tggagtcgtc gatcgggtga acgatggtcc aggaaagcgg gaagaagacg
     1201 acgcggtcgc gctccagttt gagcggaagg aactcacgat cggtgacgct ggatccgccg
     1261 cgtttccggc gcgccagcag gaccttcgcc tccatctcca cgatctcgtt gcgcttcttg
     1321 ttgacgatgc ggaacatgag cgctttgccg tcgcggtacg gcgcgacgac ggcggtacgg
     1381 ctgaagatga tctccgcccg gggccgggcg aaacgcgaaa acatgatgcc ggcgatgatg
     1441 gcaaagccga ggagcccgac cagcgattcg aaggcgacca gcacgttggc ggcgagggtc
     1501 accggaacga tgttgccgta gccgatggtg gccagcgtat gaacggagaa gaagaacgat
     1561 ctcgtgaaac gctctcccat cgactggttc gaaaaccctg tcaacgcctc ggggccgcag
     1621 gcgatgaagg caagcgcgaa gagggcgttg agcacaacgt acgcgcccga gacccaggcc
     1681 aggaagcgcg gccagctgat cgtcagaaag tagtgataga aggagagcgc ctcccaggag
     1741 ccgagccctt cgcgccgcac gttgaaggtg ccgtcgcgat tgagaaagcg cttcctgctc
     1801 tcgcgcgcca cgacgctgcc gaacccgagg tcgaggttcg gatcttcggg cggggggata
     1861 ggtgcggggg tggcagacat gacccactag attagtggag accaggcgac tagtcgatcg
     1921 gtcggatcag tcggatccgt ccgatcaacg aatggccgcc actgcgatcg ccagcagcag
     1981 cacgacgacg agggcgagcg agatccacat cgcgtggacg gttggccggt gcatgtcgac
     2041 ggtctcgagg cgctcgccga ggaggtattg gaggtattcg atgcgagggc gaacgctggc
     2101 gaccttctct tccagattgg gatagatgac ctgattgatc tccggaacga tgatcacggg
     2161 gatgaacgtg gcgccgccgg cgcgggtgac gtatccggtg agcagatcgt tgtgcggctg
     2221 gtcaccttcg gcaacgaggc agtgatagac ggtcgtcgac tgcagcacgg agagcgcttt
     2281 gcccccaacg ctgcgcgctt tgtaggcgtt gacgacgctc tcggaagtct ggtggagaat
     2341 gcgctcgttc agatcgcgga cgcgcgtgaa gatgaagatc gtctcgccgc tggcgaacgc
     2401 aatcttctcc gcgcgccgga agatgatctc gccgtagttg cgccagtcgt cgtcggtcag
     2461 gtcggtggtg aacccttcct cctccagacg ggccatgagg cggctggtgg gatcggatcc
     2521 ggcgggagac tcggggggct tttcagcttt cagctcttgc atatttttca acgaggcgga
     2581 tcaggtccgg gaactcgaat ggtttcacaa gatagtcatc tgcgccgcac tgccgcgcct
     2641 tctcggcgat gtcgcggtcg ccggagacga cgatgtacgg gatcgaccgg gtcgaatcct
     2701 gcgctttcaa cgcgctgcaa agcgccgcgc cgttcatccc tttgatggtg aggtcggcca
     2761 ggatcatctg cgggcgaagc tcgttcgcgc gcaccagtgc actcgagccg tcggagacga
     2821 cctgacactc ccggccgttg cgctgcaaca cgagcgtcac cagtcgcgcc acctgcggat
     2881 catcttcgac gacaagaatc acttcactgc tcctctaatc gaaacgaatg cgtaagcgtt
     2941 tgcggagatg accggccacg gcgccgaaga gacaacccag cgcgttccat ccggcatcgt
     3001 cccatccggt cacgcgcgtc ggcagtctgc tctgccacgc catcagcccc aacgcaaatg
     3061 tcacgcccac cgccagggtc accagatacg tccgcagccg atgctcatcc ccaccatcga
     3121 aggccaggaa ggcaagtgca ccccatggga tgaagaaaag aaggtccgca atcgccggcg
     3181 ccgccgttgc cagcagcgca tcgttggaga cggcgccggc gtcatagcgg tgaatcagcg
     3241 agaccagctc ggacatcgtg agccgcgctc ccaggtaggc gcggcccgac aagctgagga
     3301 tgagcagaga catcgccgcc gagaggatga tgagcaggat cacggtgacc gaacgcggta
     3361 cggttacgac gcggatggtc gatttgccca cggggtgatt agatcacgac atcaaaactt
     3421 cactgtccat cgttaaaata aggtcaagca atggtcaaat ccttgaccct gtgctcaact
     3481 cctgaccctc caatagtgac ttcaaagggt caggtggtat accatttacc tttgcgcaga
     3541 accgatcgaa gtcattcttt tcaacgactt aggttcgtta cagaactgtg gcaaacacat
     3601 tgctctgtga tgggaagccg atcttggtca atttcaaccc aaccactaac taaggagagg
     3661 gacccagtac atgaaaaagg ctctagtctt tgtctttgca gcagcgctcg ccacgtcagc
     3721 cttcgcgcag ggcaacagcg atccggaact gaatgccgac catggaaaca gccaggccca
     3781 cgtttcgaac ttcgccggcg gcggcggctc cggtaacctc atctaccaca ccggtggaaa
     3841 ggtgattctc aacgctcacg tggtcgagat cttctggggc tccttcccca gcggctacac
     3901 caccaacatg cagaacttcc gcaaccagtt cggcacgacc ggcgagtaca acgtgatcac
     3961 ccagtacacc ggcatcgacg acgcaacggg caccaccggt gccatcaacc tcacgaacct
     4021 cgcgggcgga ccgatcgact acttcgacac gaccacgcct ccgaccaact gcacggacgc
     4081 cgtcgtccag gcggaagtca agaaagtcat cgccgcgcag ggctcgaccg actacagcgc
     4141 catctacgag gtcttcatcc cctccggctc gtactcttcg agcggaagca gcacctcctg
     4201 cggcggaccg aacctcgcgt actgcgcgta tcacagccac tacgtcgatg gcgccggcaa
     4261 gaacgtgaag tactcgatcg agccgtgggc cggatgctcg ggctgccagg ccttcggcaa
     4321 cacgtcaacc gaccaggagc acttcgtctg ccacgaaacc cgtgaggccg tcaccgacgc
     4381 cctcggcaat gcgtggttcg accgccgcgg caacgaagcc gacgacaagt gcgcgtggac
     4441 cccgacgccg ttcatctccg gcggatacgg ctaccagtac gagtggtcga atgccaacgg
     4501 cggctgcgtc cagacccgct aatctctctc tcctaccgta acgacaaagg ccccgcgcga
     4561 gcggggcctt tttttgtgta gcggagatca ggagatcaga gacaagaaat cagtgaaagc
     4621 agaaaactcg gcgccgatct cttgtcttat ctctgatctc ttctctcgat ctcttgtctc
     4681 tgatcagccg ctgacgatac gcgcaaaacg tgatgtgtca tcctgagcct gccggaggcg
     4741 cccgcgcagc acgttgctct cgcgcagatc atcctcggcg ccgcaggaca gcgaaggatc
     4801 tcaaaatgcg aggatctcgt tcgagcggtc ttcgatcacg atcgctcgcg ctggacgaac
     4861 acgattcccg aagtttgaga tccttcgacg tcctccggca ctctcaggat gacaccaagc
     4921 tcgattgaac gtgtgaaatg agtcttagtg cggttttggc gccggcgtgt tcgaccacgt
     4981 cttctcgttc tcggtgatgt cgaagtagac cgtgtgctcg gtgtgatcgg gcacttcgaa
     5041 cgtcacgacc tcgtagcgtc gcatggcaga gccttcccca gcgtgcgcga tgccgcccct
     5101 gagtctgcgg aaacgtccgt agcggtcgta gatccaattg ttctggaagt cgattccctc
     5161 gttgggcgca ttcgccggaa cggtgacggc ggtctccggc gaatcacctt catgatcggc
     5221 cagcgcggcg gctggagccg cagcaggtgg tgtagcggca gttgcggccg cggttgatgc
     5281 aggggcagaa gctggcgccg gcgcgggcgt cgttgcgcaa gccgcggctg cggcagcgat
     5341 cgtggcgaga atgagctttc ggtgcatgcg ccgcgtcaac ctttcggcga cgacaaatct
     5401 tccgccaacc gcacgtacgc ctggtaccgc gcgttgctca actctcccgc ctcggcgcgc
     5461 tctttcaccg cgcaccctgg ctcgggcaga tgcgagcaat cattgaagcg gcagtactca
     5521 ccgggctcgg agaattcggg gaagtaatcg cgcagggttt ccagcgtcag cttccagaga
     5581 ccgaactcgc ggatgccggg agtgtcgatg agaaaggtgt tgccgccgag gtcgtggaga
     5641 gtggaggcgg tggtggtgtg gcggccggtg ccgcgtttgt gcagctcgcc ggtggcgatg
     5701 tcgagacgct catcaagtgc gttgagaatg gacgacttgc cgacgccgct gtgcccgacc
     5761 agcgcacagg tcctgccctc gacgagagcg cgcagctcgg cgatcccctc gccgctcttg
     5821 atcgagcacc ctacgaccgg tacgtcgagt gcgcgatagg gatcgagcga cgccagctcg
     5881 cgggcgcgtt cgtccggctc cagcagatcg atcttgttca cgcagatcgc cgcctctgcc
     5941 ccgccgcgct ggatggcgat gagaaagcgg tcgatgagcc gcggccgcag cggcggcgcc
     6001 ttcaccgaaa cgacgtgaat gacgatgtcg atgttcgccg cgatcagacg ctgcaggtgc
     6061 ggattgagtg gatcaggacg cgacagcctc gtgcggcggg cgagaaccgc ggccagccgg
     6121 caagtaccat cggccagcgc ctcggtaaca acgcgatcgc caaccgccag cgcgctcttc
     6181 tgccggaccg ccagctccgt cgggacgacg caatcgaatt cgcgtccgtc atagaaaagt
     6241 cggcaacgtc cgctaccgac cgagatgacc attgcggccg tgcgaggatc ggcgtctcct
     6301 gcctcccccc acgcgccgtc gtcagcggga tcatccgcag catccggctt gcggcgtttg
     6361 ccgccgcgcc gatcgccgac cggttcctcc caatcctctg ttccgcgccc gcggctgcct
     6421 cgtcttccca aacccacctc ttcgactgac agtgtaattc tccgcagccg attcgaaacg
     6481 ttccgatcac ggttttacgt aggtcggact ctccagtccg acatcacggc cagtcggact
     6541 ggagagtccg acctacgtaa cgcgagcgct aaatgaagat gaggcaatca ctcggaatgg
     6601 ctaaagttaa acacagcctc tcactgcact ccttcgtgga acggcacgag cggatcgatg
     6661 tcaacgggaa cgttcgtcgc cgcgtcgaga gccgcgcgga gctcggccgg catcttgtcg
     6721 tagcgattga accagctctc ggcgcgggcg cggtcgccgg tcgcttcgat gtcgagcagc
     6781 tccttcgcca gcgacgccag cgcgtcgggc atcttcgcgt aatcgatgcc gtagcgtccg
     6841 gacgcttcgc gcacgatcgc tcctttttcc gcgaggtagt tgaactccat catctccgcc
     6901 cgtccatgcg cctcggcggt accaaagcgc acggtgcgga agattccggc gacgtacgag
     6961 gcgtagtact cctcgagacg ctccttcggc agcgcaccgc ggtcgaccag ccacttgaga
     7021 ccgaacatgc cggtgacatc ggccttcgct tcttcgagtc ccgagtaggc cggtccgatc
     7081 gcttcgcgaa tgtcgatatg ttgacctccc ttgcgggcga aggccggtcc cagaccgtgg
     7141 gaaatctcgt gcatcatcac cgcggcaagg tatccctcac ccgaggcttg ggcggcctgg
     7201 tcgacgcgca tgatgtactt cgccagcggc agaatcacgt agttcacgcg cgcatcggtg
     7261 aagttcttga agaagatcat cttcgatccg accttctcgt ggatgcgcgg atcgttcgga
     7321 aggttgtcgg cagcggcctg atagccatgc cgcagatcgc ccgcgcggaa cggcgcgtcc
     7381 atcacttcca tcggcgaggc cagccctttc ttcgacggcc ggtcctcggc tgccaggggc
     7441 agcgcatcct gaatatccgg aacgtacttc tggaacacgg ccagcttctt cgactccgac
     7501 tcgttgcgca cgagcaccga ggcaccgtag gaagtcttca cgccgaggag gtcgtcgaga
     7561 taggtctcgt agggagcgaa aatcagatcg aacttcggat ccttcagccg cacccaggcc
     7621 agatcgctgg cgtagtaatc gtcggtgagc agcgccttgg cgcgcatgcg aaggtagttc
     7681 gcgaacgctt tgtcgtcgct ggccgcagcc gcgttgcgaa gatgacccgc ggccgcttcg
     7741 agccagtgac ggtatttgac gtgatacggc gtcgctttga gtttcagcgg agtgcggttt
     7801 gtcgcctcga cgacagaatg ttcgtcgtag atcgccttct tctccgccgg atgcgcggcg
     7861 acatatgcct cgatctcgtc gcgcgacacg cccttcggaa gaagatggcg gccgggcggc
     7921 atcggctcgc cgccgatgaa aggctcgttg tgattgagaa ggtcgtagtg gctgccgttg
     7981 agccagaggt atcgccgcgc agcctgcagc ttcgggtcgg tcgcatgtgc gaggctgttg
     8041 taaaccgcga tgtcgtcagg atcgttctgc cgccagtaga tgtcttcgag gtcgcgacac
     8101 gctgcgatca tctcgtcgac gacgcgcttc tcgcgcgcgc tcatgcccgc gaacgagaag
     8161 ggcatctcga ctttctggaa tcgggcaaga cgtttgtcga tgtcgggagc gacctgcgac
     8221 ggatcgaccg gcgttgctgc gttcagcgag actgcggcaa gaaagagaag tgcgagcttt
     8281 ttcatggctc ggcgattcta gcgcggctga ggagacacct caccacagag acacagagaa
     8341 cacagaggtt ttctctgcga cctctgtgtc tctgtggttc aaaggttttt ctagcgtcac
     8401 cgaacggttc gcgcgcgaag cgaacgatcg aactctgccg cagacaccgg ccggctgtgc
     8461 agaaagccct gcacgacctc gcagttctcg cgccgcagga agtcgagctg cgcctcggtc
     8521 tccacaccct cggcgacgac gcgcaggttg agcgcccgcg ccatcgagat cacggcggtg
     8581 atgatcgcgc catcgttcga aacgtccgat agatcgcgca cgaagttctg atcgatcttc
     8641 accgtatcga tcgggaagcg tttcaggtag ctgagcgacg agtagccggt gccgaagtca
     8701 tcgatcgaga tccggacgcc catgcttcgc agccggttca tcacggcgag cgacacgtcc
     8761 gcgttgtgca tggccgtcga ttccgtgatc tcgatgtcga gcagctcggg cggatagtcg
     8821 ctctcggtga ggatcctctc gatcatcgcc gtgagatcgc gctgctggaa ttgccgcgcc
     8881 gaaagattga ccgcaacgcg cagcagcccg tgctcgggat gccactcttt catctgccgc
     8941 acggcggttc gcaagaccca ttctccgatc ggcagaatca ggccgcactc ttccgcgatc
     9001 gggatgaagt catcgggcga catcagccct ttctccggat gattccagcg gatcagagcc
     9061 tcagcgccta cgatgtttcc ggtcagcaga ttcgtcatcg gctggtagtg gacgacgaac
     9121 tcctcccgct cgaaggcgtg atgaaggctt ctctcgatct ccaaccgttc cgaggtcatc
     9181 tcaatcgtcg tgaccggagc aaactggaag ttgtttcttc ccgcttcctt ggcgcggtac
     9241 atcgcgctgt ccgagcgccg caacagcgtc tcggcgtcgc cgccatcgtc gggaaacagt
     9301 gccacgccga tgctcgtggt ggcgtagatc tcgtgacctt cgatcaccat tggccgcgag
     9361 atcgcgtcga gcagtttctg cgcgacgatc gccgagctgc cggtttcctt gatgtcggtc
     9421 agaaggacgg tgaactcgtc gccgcccatc cgcgccactg tgtcatccgc gcggatcgtg
     9481 gccacgagcc ggttcgccac ctcctgcaga aggccgtccc cgaccgtgtg cccgagtgtg
     9541 tcgttgacga gcttgaattg atcgagatcg aggaacatca ccgcggcgtt ggttcgatgc
     9601 cgcttcgcat gggcgagcgc gatctcgatg cggtcgcgga agagcaggcg attcgggagg
     9661 cctgtgagga tgtcgtgata ggcctgatac tccatctgct cctgcgccgt cttgcgatcg
     9721 gtgatgtcga tgatcgttcc ttcgatgacg tcgccgtcga gcaggttgat gttctccagc
     9781 acccagatcg gtgtgccgtc ccgtttgcgc agacgcacct cgatgttcga caacacgcgc
     9841 tgctctttga ggcgcgacat cagccgctgc cggtcttccg gct
//
LOCUS       DATLVW010000550         6350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_434660, whole genome shotgun sequence.
ACCESSION   DATLVW010000550 DATLVW010000000
VERSION     DATLVW010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434660"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1257
                     /locus_tag="VNN25_16435"
     CDS             70..1257
                     /locus_tag="VNN25_16435"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXH93172.1"
                     /translation="MIRIRTLDDLSLSAGARVFYRVDYNVPLDGTRIADATRIEETLP
                     TLRRLRDAGAAIVIASHLGRPKGERKTKYSLDPVREKLAELLGAEVQWAADCIGDEAE
                     SKASKLKAGQMLLLENLRFHAGEEANDAAFAAQLRKLADYYVNDAFGACHRAHASIDA
                     LPRLFETNHTAGGLLLAKEIEFLQKITNVSERPFVALLGGAKIAGKIEPLEALVKLAD
                     TVLIGGGMANTFLAARGVPMGGSLVDKDSMEVAKRIQNVTLPGDLIVADDLESPTSIE
                     TVAVAEGLTTGQKAFDIGPKTIAQYDNALKSARTIFWNGPMGVFEKEAFAKGTMAMAR
                     SVAEADAITVVGGGESVEAVKASGFANRISHISTGGGASLEFISGASLPGVEVLRMMK
                     AEG"
     gene            1317..1874
                     /gene="cyaB"
                     /locus_tag="VNN25_16440"
     CDS             1317..1874
                     /gene="cyaB"
                     /locus_tag="VNN25_16440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HXH93173.1"
                     /translation="MATEIEVKFALKDRAELVSKLHEIGGQRLYPETFEDNIVLDRRG
                     DLKTRGSLLRVRKFGKYSIATYKGPMSIEGGVKSREEVQTGVESFELAIQLFDALGYK
                     PVFRYQKFREVWRIRDVEVVLDRTPIGDYFEIEGPIDVIRTVTAELGMTMDQAIRQTY
                     SDLYRLARRTRPDLGENMIFTPDQL"
     gene            1880..2893
                     /locus_tag="VNN25_16445"
     CDS             1880..2893
                     /locus_tag="VNN25_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXH93174.1"
                     /translation="MILAAGYGTRLRPVTYTIPKPIVPLCGRPLMAWAVESLLAAGIR
                     DIVVNLHHLPEAIERYLREAYAGEARFEFSYEAEILGTGGGVRRVRPLLEADDEFFLV
                     NGDTIQFPPYDALRSVRRESDALAALTLRHPPQGDRFTPVWFEENLVTGFGNGTGEPL
                     MFAGTHLISRRIFDYLPDKEFSGIVDEVYQPLIESGKERVAGVVDDGSLWFDIGTPLR
                     YIAASRALLARTVDGTLAPSRETHVAGDSLVHDTASVRGRCTQSIVGARSTSEGDLRD
                     SAVWDDCRIAPGVVLESCVVAHGVEVSTPVELRNVLICRDESAIPRDGECRFEHGLAL
                     RDI"
     gene            3052..5220
                     /locus_tag="VNN25_16450"
     CDS             3052..5220
                     /locus_tag="VNN25_16450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93175.1"
                     /translation="MPHGSDSAAQRRTITVFITQGLAGLLLMLWVTWLRFDQTAAIFR
                     NLQPNAIVVIAILVAFATTLGLLKFELTELIFVSLVLTAYTVMIPILGVVAASWLAVI
                     VAAFARFLALKQIGPAKIESTDRFLDHVKTFGLFGTYGIPVVAAATLYERLHGELPLA
                     HATLGAALKIALCGVVLSVTNILLMFRVAQSYGYSLRKIIRLDGVDLTIYLLTLPYAV
                     ITALGFAAMGWGALIALAFSGSIAVWMARMLAKTRMKSVQQLQRIASLTNIGKTISLR
                     YTTDQLLSAIYNECKRSVDCTLFTIALLDESTNELSFELDVREDIRLPVERIPVGEGL
                     NSWVVNHHEPLLIGSTADEKRLGVMAVADTKPTQSWLGVPMIARDRVIGVISVESFKK
                     NAFTPDDMLLLTAIANQAAVAIENAHLYKDLEGLTYALEQRVMERTNELRETNLRLLA
                     ADRSKNQFLANMSHELRTPLNSIIGFSSVLLENTGSTLPSRFYKFLENIHVAGNHLLD
                     LINDILDLSKIEAGKMELRTDEFDLRDTIASVERVMKGFAAEAKVSISATIDPAVPRV
                     RLDEGRLKQILFNLLSNAVKFSPRGGPVTIAVSSIPAASSPLDVLTVRIDVTDEGIGI
                     APDELQRIFDEFYQTEEGRRARRGGTGLGLSLTRNFVELHHGRIEVQSALGRGTCFTL
                     YLPIDYDEAAPESGVGSRESEHRLTTPDPRPPTPRVLQQL"
     gene            complement(5206..5622)
                     /gene="ndk"
                     /locus_tag="VNN25_16455"
     CDS             complement(5206..5622)
                     /gene="ndk"
                     /locus_tag="VNN25_16455"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXH93176.1"
                     /translation="MANRTFTIIKPDSVRKGNFGKIISRLESEGFRILGLKKVSLSTK
                     QAQGFYAVHSERPFYPDLVKYMTSGPVYVAALEKEGAVPALRKLMGATDPSKADAGTI
                     RKDFGESIEQNAIHGSDSDENAAIEIGFFFPESELL"
     gene            5841..>6350
                     /locus_tag="VNN25_16460"
     CDS             5841..>6350
                     /locus_tag="VNN25_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93177.1"
                     /translation="MERGRCFERSPRRGGIEGSKQTIVRIDRRRFKTDRRWFKKSRRW
                     LKRTVDGRKEPSMAEKSRRWLKRAVDGRKGPVDGRKEPSMDQNEPSMDQNEPSMDENE
                     PSMDENEPSMDQNEPSMDENEPSMDQNEPSMDENEPSMDENEPSMDENEPSMDENEPS
                     MDENEPSMDQ"
BASE COUNT         1313 a         1896 c         1937 g         1204 t
ORIGIN      
        1 ctattcgaat cgaattctcg atctgatcgc ctacgttgga cgggtgaaga aggacgcctc
       61 gcgaagcgca tgatccggat ccgaacgctc gacgacctgt cgctctccgc cggagcacgc
      121 gttttctatc gcgtcgatta caacgtgccg ctcgatggca cgcgtatcgc cgacgcgaca
      181 cggatcgaag agacgctgcc gacgctgcgt cgtctgcgcg atgccggcgc tgccatcgtg
      241 atcgccagtc acctcggacg cccaaaaggt gagcggaaaa cgaagtactc gctcgatccc
      301 gtgcgcgaga aactggccga gctcctcggc gccgaggttc agtgggcagc cgattgcatc
      361 ggcgatgaag ccgagtcgaa ggcgtcaaaa ctgaaagcgg gccagatgct gcttctcgaa
      421 aaccttcgct ttcatgccgg cgaggaggca aacgatgccg ccttcgctgc gcaattgcgc
      481 aaactcgcgg actactacgt caacgatgcc ttcggagcgt gtcatcgcgc gcatgcgtcg
      541 atcgatgcat tgccccggtt gttcgaaacg aatcacacgg cgggcggttt gctgctggcg
      601 aaagagatcg agtttctcca gaagatcacc aacgtctccg agcgtccgtt cgtcgccctc
      661 ctcggcggcg caaaaatcgc cggcaagatc gagccgctgg aagcgctcgt gaagcttgcc
      721 gacaccgtgc tgatcggcgg gggaatggcc aacaccttcc ttgccgcccg cggcgttccg
      781 atgggtggct cactcgtcga caaagactcg atggaagtgg caaagcgaat ccagaacgtg
      841 accctgcctg gcgatctgat cgtcgccgac gatctcgaat cacccacgtc gatcgagacg
      901 gtggccgttg ccgaggggct tacgacggga cagaaggcgt tcgacatcgg cccgaaaacc
      961 atcgctcagt acgacaacgc gttgaagagc gcgcgtacca tcttctggaa cggaccgatg
     1021 ggcgtcttcg aaaaggaagc cttcgccaag gggacaatgg caatggcgcg atccgtcgcc
     1081 gaagccgatg caatcacggt cgtcggcggc ggcgaatcgg tggaagccgt gaaggcatca
     1141 ggatttgcta atcgcatcag ccacatctca accggcggag gcgcatcgct ggagttcatc
     1201 tcgggagcgt cgttgccggg agtggaggtg ttaagaatga tgaaggcgga aggatgaagg
     1261 atgaatcgga gcgacccgcc ctcacccttc atccctcatc attcatcctt taatccatgg
     1321 ccacagaaat cgaagtcaag ttcgccctca aagaccgcgc tgagctcgtg agcaagctgc
     1381 acgagatcgg tgggcagagg ctctatccgg agacgttcga ggacaacatc gttctcgacc
     1441 ggcgcggaga tctgaagacg cgaggctcgc tgctgcgcgt acgcaagttc ggcaagtact
     1501 cgatcgcgac ctacaaaggg ccgatgtcga tcgagggtgg cgtgaagtcg cgcgaggaag
     1561 tgcagaccgg cgtggagagc ttcgagcttg ccatccagct cttcgacgcc ctcggctaca
     1621 aaccggtctt ccgctaccag aagtttcgcg aggtatggcg catccgcgac gtcgaggtcg
     1681 ttctcgaccg cacacccatc ggcgactact ttgagatcga aggtccaatc gacgtgattc
     1741 gaaccgtcac cgcggagctc gggatgacga tggatcaggc catccgccag acgtactcgg
     1801 atctgtatcg cctggcgcgg agaacacgcc ctgacctcgg ggagaacatg atcttcacgc
     1861 cggatcagtt gtgaaagcaa tgatccttgc cgccggctac ggcacgcggc tccgtccggt
     1921 cacctacacg attccgaaac cgatcgtgcc gctgtgcggc cggccgctga tggcctgggc
     1981 cgtcgagtcg ctgcttgctg ccgggattcg agacattgtc gtcaatctcc atcatcttcc
     2041 cgaggccatc gagcgctatt tgcgcgaggc gtatgccggc gaggcgcgct tcgagttttc
     2101 ttatgaggca gagatcctcg gcaccggcgg aggcgtgcgc cgcgtgcggc cattgctgga
     2161 agcggatgac gagttctttc tcgtcaacgg cgacacgatc cagttccctc cttacgacgc
     2221 cctgcgttcc gttcggcgcg aaagcgacgc gctggctgcg ctgacgttgc ggcatccgcc
     2281 gcagggtgac cgcttcacgc ccgtctggtt cgaggagaat ctcgtcaccg gctttggaaa
     2341 cggtaccggc gagccgctga tgttcgcggg cacacatctg atctcccgcc gcatctttga
     2401 ctacctgcca gacaaagaat tctcgggcat cgtcgacgag gtttatcagc cgctgatcga
     2461 aagcggcaag gaacgggttg cgggagtcgt cgacgacgga tctttgtggt tcgacatcgg
     2521 cacgcctctc cgctacatag cagcctcgcg cgcactgctc gcacggacgg tcgacggcac
     2581 gctggccccc tcccgcgaaa cgcatgtggc gggcgactcg ctcgtgcatg acacggcgtc
     2641 ggtgagaggc aggtgcacgc aaagcatcgt cggcgcccga agcaccagcg aaggagatct
     2701 tcgcgactcc gccgtttggg acgactgccg gatcgctccg ggcgtcgttc tcgagtcgtg
     2761 cgtcgtcgcg cacggcgtgg aagtctccac tcccgtcgag ctgcgcaacg tactcatctg
     2821 ccgagacgaa tcagcaatcc ctcgagacgg cgaatgccgc ttcgagcatg gtctggctct
     2881 ccgcgacatc tgatcgacga cttcaaagag cacagacaag agtgtctgtg ccacatcagt
     2941 ccgccgaaac atggatcgtt gtcccgaagt agcaaggagg caggagcctt ctgtccgagc
     3001 agtgcggccc gggttccggt gctacgataa ctgacgggtt cactcactcc gatgcctcac
     3061 ggttccgact ccgccgccca acggcgcacg atcaccgtct ttatcactca gggcctggcc
     3121 ggcctgctgc tgatgctctg ggtgacgtgg ctgcgcttcg accagacagc ggcgatcttc
     3181 cggaaccttc agccgaacgc catcgtcgtg atcgcaatcc tcgtcgcgtt cgccacgacg
     3241 ctgggactcc tgaagttcga gctcacggag ctgatctttg tctcgctcgt gctgacggcg
     3301 tatacagtca tgatcccgat tctgggtgtc gtggcggcat cgtggctggc ggtcatcgtt
     3361 gccgcgttcg cgcgctttct cgcgctcaaa cagatcgggc ccgcaaagat cgagtcgacc
     3421 gaccgcttcc tcgatcacgt caagaccttc ggccttttcg gaacctacgg cattcccgtg
     3481 gtcgccgcgg cgacgctgta cgagcggctc cacggcgagc ttccgctggc tcacgccacc
     3541 ctcggcgccg cactgaagat tgcactctgc ggcgtcgtcc tgagcgtcac gaacatcttg
     3601 ctcatgttcc gcgtggcgca gtcgtacggc tattcgctgc gcaaaatcat ccgtctcgac
     3661 ggcgtggatc tgacgatcta tctcctgaca ctgccgtatg cggtgatcac tgcgctcgga
     3721 tttgcagcca tgggctgggg cgcgctgatc gccctcgcct tcagcggaag catcgccgtc
     3781 tggatggcgc gcatgctggc caagacgagg atgaagagcg tgcagcagtt gcagcgcatc
     3841 gcctcgctca ccaacatcgg caagaccatc tcgctgcgat acaccactga tcagcttctc
     3901 agcgcgattt acaacgagtg caaacgctcc gtcgactgca cgctttttac gatcgctctc
     3961 ctcgacgaat cgaccaacga gctgtcgttc gagctcgacg tccgtgaaga catcaggctg
     4021 ccggtggaac ggattccggt tggcgagggc ttgaactcct gggtcgtcaa tcatcacgag
     4081 ccgctcctca tcgggagcac cgcggatgaa aaacggctcg gcgtgatggc cgtagccgac
     4141 acgaagccca cgcaatcgtg gctcggtgta ccgatgatcg cccgcgaccg cgtcatcggc
     4201 gtgatctcgg tcgagagctt caagaaaaac gccttcacgc ccgacgacat gctcctgctc
     4261 accgcgatcg cgaatcaggc ggcagtggcc atcgagaatg cgcatctcta caaagatctc
     4321 gaagggctta cgtatgcgct cgaacagcgc gtcatggagc gcacgaacga gttgcgcgaa
     4381 acgaacctcc gcctgctcgc cgccgaccgc tccaagaatc agttcctggc caatatgtcg
     4441 cacgagttgc gaacgccgct gaactcgatc atcggtttct ccagcgtgct gctcgagaac
     4501 acgggcagca cgcttccgag ccggttctac aagttcctcg agaacattca cgtcgccggg
     4561 aaccatcttc tcgatctgat caacgacatc ctcgacctct cgaagatcga agcgggcaag
     4621 atggagctgc gcacggacga gttcgacctg cgcgacacga tcgcgtccgt ggagcgggtc
     4681 atgaagggat ttgccgccga ggcaaaagtg agcatcagcg cgaccattga tcccgcagtg
     4741 ccacgcgtcc ggctcgatga gggccgcctg aaacagatcc ttttcaacct gctgtcgaac
     4801 gctgtgaaat tctcgccgcg cggcggtccc gtcacgattg cagtgagctc gattcctgca
     4861 gcgtcatcac cgctggatgt gctcacggtg cggatcgacg tgacggacga agggatcggt
     4921 atcgcgcccg acgagctgca gcggatcttc gacgagttct atcagaccga ggaagggcgc
     4981 cgagcgcggc gaggcgggac cgggcttggt ttgtcgctca cgcgcaactt cgtcgagctg
     5041 catcatggcc gcatcgaggt tcagtcagct ttgggacgag gcacctgctt cacgctttac
     5101 cttccgatcg actacgacga agcagcaccg gagtcgggag tcggtagccg ggagtcggag
     5161 catcgcctta cgacccccga cccccgaccc ccgacacccc gcgttctaca gcagctctga
     5221 ttcggggaag aaaaagccga tctcgatcgc cgcgttttca tccgagtcgg atccgtggat
     5281 ggcgttctgc tcgatcgact cgccgaagtc tttgcggatc gttccggcat cggctttcga
     5341 aggatcggtc gcgcccatga gcttgcgaag ggcgggaacc gcgccttctt tctccagtgc
     5401 ggcgacataa acgggtccgg aagtcatgta cttcacgagg tcggggtaaa acggccgctc
     5461 gctgtgcacc gcgtaaaagc cctgagcctg cttcgttgag aggctgacct ttttcaatcc
     5521 gaggattcgg aagccctcgg actcgagccg gctgatgatt ttgccgaagt tgcctttgcg
     5581 gacggagtcc ggcttgatga tcgtaaacgt gcgatttgcc atgagtagtt gtccttgcgg
     5641 tttgaaatgg gcggcggatt atagctggtg cgggggtcgc tggtcgggag tcgggggtcg
     5701 gagagatcag agattcagag attagagatc agagattcag agatcagaga ttcagagatc
     5761 agagatacgg cagatcgcca attgaatagc gttaggtcat tgcgacgaga cgattggtcg
     5821 tgtgcgcctg agagcaggga atggagcgag gccggtgttt tgagcgctct ccccggaggg
     5881 gcggcatcga aggttcgaaa cagacgatag tccgaatcga ccgccgacgg ttcaaaacag
     5941 accgtcgttg gttcaaaaag agccgtcgat ggctgaaaag aaccgtcgac ggccgaaaag
     6001 aaccgtcgat ggccgaaaag agccgtcgat ggctgaaaag agccgtcgat ggccgaaaag
     6061 gacccgtcga tggccgaaaa gagccgtcga tggatcaaaa cgagccatcg atggatcaaa
     6121 acgagccgtc gatggatgaa aacgagccgt cgatggatga aaacgagcca tcgatggatc
     6181 aaaacgagcc gtcgatggat gaaaacgagc cgtcgatgga tcaaaacgag ccatcgatgg
     6241 atgaaaacga gccatcgatg gatgaaaacg agccatcgat ggatgaaaac gagccgtcga
     6301 tggatgaaaa cgagccatcg atggatgaaa acgagccgtc gatggatcaa
//
LOCUS       DATLVW010000551         2341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_477283, whole genome shotgun sequence.
ACCESSION   DATLVW010000551 DATLVW010000000
VERSION     DATLVW010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477283"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VNN25_16465"
     CDS             complement(<1..596)
                     /locus_tag="VNN25_16465"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXH93178.1"
                     /translation="MTSLLSEPRQPAIAESPLAEPRSGLLDLAASLCQARFAVLRIDG
                     RDATANDPTVPLDAEEVLAACALKRDEPFVVLDDKDLESVSGIGFYAAVRLDDGASGI
                     HGTLSVLDKRARRLTKQEQQLLIKVAAQIVRSVRNEEILRASSEQQRWMQALVEQSPV
                     AMYTVDGGRLSFVNARFASALGYTKEQVLALGSITEIIV"
     gene            762..2129
                     /locus_tag="VNN25_16470"
     CDS             762..2129
                     /locus_tag="VNN25_16470"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HXH93179.1"
                     /translation="MDLSVTYDTPLLTALGQLGPHDHQSLIYENPDDRFSVAIPFIRI
                     GLDRGEKCVYIADDGKEAAVHEAMHAQGIDVERAIASESLVLETTKSVFLKHGYFDAD
                     WMPEFWGDAAAEATRQGFSALRATGETEWLFGAPQGGDRWMEYESRLTHVLAGLNCFA
                     LCQYNRQVLPPELILDVIRTHPTVIYRGVVCRNMYYVPPDEFLGANQAAHEAERLLAT
                     LRERAEVELTLRQQRDELRSLANRLMHAQDDERRRLATMLHETTAQDLAALKMLLTRL
                     NRESGGLGDSDRHDLAESISLTEESIAGIRTVSYLLHPPFLDEAGLVSALRWYAGGFA
                     ERSGIKVELDLPENLDRLPLDTETVLFRVVQESLTNIHRHAESETARIQLRRDAGMIS
                     LAIEDRGHGIPSGTLEQITSGGVGVGIAGMSERIEQLGGRLEVTSSGKGTIVRVWVPV
                     EKDAN"
     gene            complement(2193..>2341)
                     /locus_tag="VNN25_16475"
     CDS             complement(2193..>2341)
                     /locus_tag="VNN25_16475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93180.1"
                     /translation="TESQRTGESSIGLLGMRERAHLIGGEIEVSGIEGEGTTVTLRLP
                     IEAP"
BASE COUNT          471 a          714 c          730 g          426 t
ORIGIN      
        1 acgataatct cggtgatcga gccaagcgcg aggacttgtt cttttgtgta cccaagagcc
       61 gaggcaaacc tggcgtttac gaatgagaga cggccgccat cgacggtgta catcgctaca
      121 ggcgattgct caacgagagc ctgcatccag cgctgttgtt cactggatgc tcgaaggatc
      181 tcttcgtttc gaacggagcg tacgatttgc gctgcgactt tgatcagcag ttgctgctct
      241 tgtttcgtca gtcgccgcgc ccgtttgtcg agcacggaga gtgtgccgtg aatcccactg
      301 gcgccatcat ccaggcgaac cgctgcatag aaaccgattc cggaaacgga ttccaaatcc
      361 ttgtcatcga gaacgacgaa gggttcgtcg cgcttgaggg cacacgcggc gagcacctcc
      421 tcagcatcca gaggcacggt tggatcgttg gccgtcgcgt cccggccatc gattcgaagc
      481 acggcgaacc gcgcctggca cagcgatgcc gccagatcga gcaagccgct cctcggctca
      541 gcaagaggag actccgcaat ggcaggctgt cgcggctcgg agagcaagga ggtcatcgct
      601 cgattcaaac gtgcgccaaa gaacgcgaca tccctactgc gagcgatctt gtctgaatgg
      661 cttgtaggaa gaattccgat tcacgcacaa atcgagcgcg acaacggacg cgtccgttgg
      721 gtccaggaaa tgcaacgaga agagacgcgt tggagggtgt tatggacttg agcgtcacct
      781 acgacacacc gctgctgacg gccctcggac agttgggtcc ccacgaccac caatctctga
      841 tctacgagaa tccggacgat cgcttctccg tggccatccc attcatccga atcggtcttg
      901 accgcggcga gaagtgcgtc tacatcgccg acgacgggaa ggaagcggcc gtgcatgagg
      961 cgatgcacgc ccaagggatc gacgtcgaac gggccatcgc cagcgagagt ctcgtgctgg
     1021 agacgacaaa gagtgtgttt ctcaaacacg gatacttcga cgccgattgg atgcccgaat
     1081 tctgggggga cgcggcggcg gaagccacgc gtcaggggtt ttctgcgttg cgcgcgaccg
     1141 gcgaaaccga gtggctcttc ggcgcgccac agggaggcga tcggtggatg gagtacgaga
     1201 gccggctaac gcacgtgctg gccggtctca attgttttgc gttgtgccag tacaaccgtc
     1261 aggtccttcc acccgagctc atcctcgacg tgatccgcac acatcccacg gtcatttatc
     1321 gcggcgtcgt ttgccggaac atgtactacg tgccccccga tgaattcctc ggcgccaacc
     1381 aggcggccca tgaggccgaa cgcctgctgg cgacgctccg cgagcgcgcg gaggtcgagc
     1441 tgacactccg gcagcaacgc gacgagttgc gctcgcttgc gaaccgtctc atgcacgccc
     1501 aggacgacga acgccgccgc cttgcgacaa tgcttcacga gaccaccgcg caggatctgg
     1561 ccgcgctgaa aatgctcctc acgcgattga accgggagtc cggcggtctc ggtgacagcg
     1621 accgccatga cctcgcggaa agcatctctc tgaccgagga gtcgatcgcc ggaatccgga
     1681 cggtctccta tctgctgcat ccgccgtttc tcgacgaggc cggtctggtt tcggcactac
     1741 ggtggtacgc cggcggcttt gcggagcgca gcggcatcaa ggtggagctg gacctgcccg
     1801 agaatctcga tcggctgccg ctcgacaccg agacagtgct ctttcgcgtc gtgcaggagt
     1861 cattgaccaa cattcaccgg cacgcggaga gcgagaccgc tcgcatccaa ctgaggcgtg
     1921 acgccgggat gatttcgctg gcgatcgaag accgaggcca cggaatccca agcggcacac
     1981 tcgagcaaat cacgagcggc ggcgtcggcg tgggcatcgc cggcatgagc gagcgcatcg
     2041 agcagctcgg cgggcgcctg gaggttacct cgagcggcaa aggtacaatc gtacgagtgt
     2101 gggtaccggt tgagaaggac gcaaactgac ggcgaagcag tagcgcggtt gccaggctgc
     2161 gcgaggtctc gcggtgtctg agctgctccg tttcagggag cttcaatggg caaccgcaga
     2221 gtaacggtcg tcccctctcc ttcgatgccg ctaacttcaa tctcgccgcc gatcaggtgc
     2281 gcgcgctcgc gcattcccag aaggccgatg gacgactcgc cggttcgctg actttccgtg
     2341 a
//
LOCUS       DATLVW010000552        10248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_68110, whole genome shotgun sequence.
ACCESSION   DATLVW010000552 DATLVW010000000
VERSION     DATLVW010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68110"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VNN25_16480"
     CDS             complement(<1..1330)
                     /locus_tag="VNN25_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXH93181.1"
                     /translation="MFEKYNEKARRALFFARYEASKLGSRVIESEHILLGVLREGEEI
                     IKEIFSRFNVKPEQIRREVEGDRLFVDRISSSAELPLSEESKKILAYAAHEAESMLHQ
                     YVGTEHLLIGILRVESSTAARILTAKGLNVYGVREETISILKEREADKQKKELPFLAE
                     YARDLTAMAHSAQFDPLIGREKEVERIIQILSRRTKNNPILLGEPGVGKTAIVEGLAQ
                     RIVDGNVPLFIANKRILSLDLSLIVAGTKYRGQFEERLKGIIKELKENTDIIIFIDEI
                     HSLIGAGSAEGSLDAANILKPALSRGEISCIGATTIREYRRYIEKDRSLLRRFQAINV
                     APPNEIETLQILEGVKERYESFHKVKYSDTAIRSAVYQSNRYITDRFFPDKAIDILDE
                     AGAKVKLRRVADTQNLRRLESEIRSIVKEMKKAISDKDFEKAVFLREREIE"
     gene            complement(1425..2105)
                     /locus_tag="VNN25_16485"
     CDS             complement(1425..2105)
                     /locus_tag="VNN25_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93182.1"
                     /translation="MPFLEARSLSKTYRNAEVPVPVLSGLELTVERGEMLAIVGPSGI
                     GKSTLLHLLGGLDHPDSGTIRVGDAEVSSMSNDELARFRNHNVGFVFQFHHLLPEFTA
                     AENVAMPGWIGRFGKDEALSKANALLAELGLEHRSRHFPNQLSGGEQQRVAIARALLA
                     DPLLFLADEPTGNLDLETSERVFDLMRECHAKRGLTSVIVTHNRDLAARCDRVFEMRQ
                     RVTAEALS"
     gene            complement(2187..3863)
                     /locus_tag="VNN25_16490"
     CDS             complement(2187..3863)
                     /locus_tag="VNN25_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXH93183.1"
                     /translation="MEQLQLHYVDLGVPRVMDLKHELSVGRTEGNDLILNHPSVSRKH
                     AKFELRGDHWWVIDLKSTNGVKVNGNLVTEAQVAAGEKLSIGSVLIDVRALPSVDFNA
                     DSMFDNPSGTVIRRISDFNSEFGLDIAELADKGPVTRPPSQPGIREPAVSREKIFQVL
                     VQVAKALLASEELQVVLTTVMDIIFRYLPVERGLIILFDEDGNPVPKLTKFIEGAEQQ
                     DIPISRTILKMVSQQQVALMTSNALEDARLLGGKSIAIHGIRSAMCVPLWNRQRVIGA
                     VQVDSPIHIGSFTEEDLDLLTALANFAAVAIERAQLAEKIEEEKKIRSRMERYHSPSV
                     VDEIVKGSISTETEVRSTDVSILFADISGFTPMSESKKPEEVAEFLSHFFGFAVESIF
                     AYGGTLDKFIGDAVMAFFGAPLPQDDHADRAVLTGLMLQRLVNDWNVERAKTELPPVM
                     VRVGINSGLAVVGNVGTEKRVDYTVLGSSVNIASRLESGVAKPGQVVVSQNTMDRIMG
                     SFETESLGEFALKGLQQKMPVFHVKGTVGLSGSGTPAGGTPALQSTSIAR"
     gene            complement(3917..7315)
                     /locus_tag="VNN25_16495"
     CDS             complement(3917..7315)
                     /locus_tag="VNN25_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXH93184.1"
                     /translation="MGERFSFDVFVSHSSKDKSIVRALAERLRADGLRVWLDDWEIRP
                     GDSIPAKIEEGLEHSRVLVLCMSANAFGSDWAQLEAGTFRFRDPLNKERRFIPLRLDD
                     SPVKASLAQFLYVKWPPKDRMQEYANLLEACRPPAKPKVADVEAVGEQLAEKSIQLDE
                     ERGILAYGFSPDGRRALTGAGGNTVRLWDVGTGRCLRVMKGHSADVWCIAWSNDQRRA
                     LSGGDDNDIRIWEVETGRCLRLLEGHAGRVMGVVWSIDNRRALSGSYDGTIRLWDVET
                     GRCLRKMKGQPSEIWGLSWSADGRRALTGGQDNVVRVWDVHTGRCLHVLEGHTESVHH
                     VSISVDGRFGLSGSNDSTVRLWDMETGRCLGVMKGHSQSVRPVIWSKGGHALSGSFDG
                     AIRLWDVETGLCLRVLKGHTDDVRTLAFSPDGRSAFSGDYAGGIRVWDLSEFGKVARA
                     TIHTGPALPPAPDQVQYTNAKVLLVGDSGVGKSGLSNYLAHDIKVEDDKPLPSTDGAW
                     ATHWPLHHEKKKAGVEREIWLWDFAGQVDYRLVHQLFMDDTAAAVLVFNPQNENPFEG
                     LGQWDHDLQKAARRPFAKLLAAGRVDRGGLVGSANNMKKFMAEHGFLQPLHLTSAKTG
                     EGCDSLRKAIVKAIDWTSIPETTSPALYHRIKLETLSLRDQGLVLIRLSELKQRMEMT
                     LRNEDFELGELEAVVSLLSGPGMIQRVDFGGFILLRPEVLSRYAAAVVRKVRKHPQEM
                     GCIREDELLAANLDYQDFERLPREDEAVVLRALLETFISRAWCLREPFDGTAMLTFPS
                     YFRRERKEQPSHPSVLVTYRFEGPTDDMYATLVVRLHHSGAFESTNLWKSAADFKTQT
                     GAALGFTLTRETEGASRLQVYFEPDVDANSRVLFDSYVHNHLTQQSRSVIRLRHYACG
                     NKKCDSFGQAFIDQTKIDKALAPGGKGKVFCPDCGKPILLRDAIEEKYNSRAVKERVR
                     VMQYEGQAVIDNESRELQAVHHTGFIVAESGQIYRGYTNSDHGIDGEIEFKDDQSRAS
                     GKRLYVQLKSGDSYLKKRKRDGAEVFQIKNPRWADYWQQHAYPVMLVIRTSDGEIRWM
                     DVSAYLKRECADRKAVKQVVFEGELFDVMSVRRWREKALDLPAS"
     gene            complement(7455..8825)
                     /locus_tag="VNN25_16500"
     CDS             complement(7455..8825)
                     /locus_tag="VNN25_16500"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH93185.1"
                     /translation="MTTIDRTTAPQPGAPRPYHFPNVVRRTLDNGLRLLVTENHNAPL
                     VSMRTLVRSGADYDTPELAGLASLTSELLDEGAGSRDAVRLAEDLGLLGASLGSGADW
                     DASYVSVDSLSRTFDEALAIFADVNRRPILPESSLDRVRSERLMELLQQKDDPGAIAG
                     KRFSHLIYGRGTYGNTVIGNPESVANIKVDDVRRYYATHFVPNNSSIVVAGDVDAEKA
                     LGGLTRFFADWPVVEVPPRPKISPKANEASRIYLIDRPTAVQSEIRVGHIGIARSTED
                     YFALSVMNALLGGVFNSRINLNLREKHGYTYGARSVFAFRRQAGPFVVSAPVRNEVTR
                     ESVTEVLSELRRIRTGDVEERELEDTKNYIMGSFPSTVQSSSDIAGRVLDMELNDLPQ
                     DYFDRYRENIGAVSKEAVEHVAQKYIDPDRVIVVIVGNAAQIREPLVALGMPIHELDI
                     EGQAIA"
     gene            complement(8822..10078)
                     /locus_tag="VNN25_16505"
     CDS             complement(8822..10078)
                     /locus_tag="VNN25_16505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH93186.1"
                     /translation="MLSIETYTLDNGLRVVLNEDHSAPLVAVNLWYHVGSKNERPGRT
                     GFAHLFEHMLFSGSEHVGNNEHFRYVQSIGGVLNGTTFFDRTNYFETLPSNYLALGLW
                     LESDRMGFFLPALTQEKLDIQREVVKEERRQRYDNVPYGTAFERLLNLAYDPDYSYHW
                     PTIGSMEDLGAASLDDIREFFSTWYRPDNATLTLIGDFVPEEAKELVEHYFGSIPAGG
                     SFPKFALTRKPLNGERRETVPSPVQLPRVYRLYHLPKMGHPDWIAGDLLSSVLSGDKA
                     SRLEKALVLEQQIATDVAAYVLPTEATGILMIQATANDDVPIEDVERAIDAEIVRVVS
                     DGITADELTRAKNRAEVEYAHQIENYDSRADLIGMMATYFDDPSLVHTWLDPYRAATS
                     ADLQRVTREYLVPDNRVTSTFVPENA"
BASE COUNT         1963 a         3113 c         3062 g         2110 t
ORIGIN      
        1 gctcgatctc gcgctcgcgc aggaaaacgg ccttctcgaa gtccttgtcg gagatcgctt
       61 tcttcatttc cttgacgatc gagcggatct ccgactcgag ccggcgcaga ttctgcgtgt
      121 cggcgacgcg gcgcagcttc accttcgcgc cagcttcatc gaggatgtcg atcgctttgt
      181 cggggaaaaa gcggtcggtg atgtagcggt tcgattggta gaccgcgctg cgaatggccg
      241 tgtcgctgta cttcactttg tggaagctct cgtagcgctc cttcacccct tcgaggattt
      301 gcagggtctc gatttcattc ggtggggcaa cgttgatggc ctggaagcgg cgcagaagcg
      361 agcggtcttt ctcgatgtag cggcggtact cgcgaatcgt ggttgcgccg atgcaggaga
      421 tctcgccgcg cgacaacgcc ggcttgagga tattggcagc gtcgagcgac ccctcggccg
      481 agcccgcgcc gatgagcgag tggatctcat cgatgaagat gatgatgtcg gtgttttcct
      541 tcagctcttt gatgattccc ttgagccgct cttcgaactg gccgcggtac ttcgttccgg
      601 ccacgatgag cgagaggtcg agcgagagaa tgcgcttatt ggcgatgaag agcgggacgt
      661 tgccgtcgac gatgcgctga gcgagacctt cgacgatggc ggtcttgccg acgcctggct
      721 cgccgaggag gatggggttg ttcttcgtac gccgggagag gatctggatg atgcgctcga
      781 cttccttctc gcgtccgatg agcggatcga actgcgccga atgcgccatc gctgtcagat
      841 cgcgggcgta ttcggcgaga aacggcaact ccttcttctg cttgtcggcc tcgcgctctt
      901 tcaggatcga gatcgtttcc tcgcgcacgc cgtagacgtt gagccctttg gcggtgagta
      961 tccgcgcggc tgtcgatgac tccacgcgga ggatgccgat cagcaggtgc tctgtgccga
     1021 cgtattgatg aagcatcgac tccgcttcgt gcgccgcgta cgccagaatc tttttcgact
     1081 cctcggaaag cggcaattcg gccgaggagg agatgcggtc gacgaagagg cgatcgcctt
     1141 caacctcgcg ccggatctgc tccggcttga cgttgaaacg cgaaaagatc tccttgatga
     1201 tctcttcccc ttcgcgcagg accccgagga ggatatgttc ggactcgatg acgcgggagc
     1261 ccagcttgga ggcttcgtag cgggcaaaga aaagtgcgcg gcgtgctttc tcgttgtatt
     1321 tttcgaacat gaggttgggt atagtttacc gccgctccgt cataacttcg ttccggcctc
     1381 cgtcattctt gggcaatcac tccgttctgt gacggccggc actctcacga cagggcctcc
     1441 gcggttacac gctgcctcat ctcgaacacg cggtcacagc gcgctgcaag atcacgattg
     1501 tgcgtgacga tcaccgaggt cagtccacgc ttggcgtgac attcgcgcat cagatcgaag
     1561 actcgctcgc tcgtttcgag atcaagattt ccggtcggtt cgtcggcgag aaaaagcaac
     1621 ggatcggcga gaagcgcccg agcgatggcc acccgctgtt gttcgcctcc cgaaagttga
     1681 ttcggaaagt gacggctccg atgctcgaga cccagctccg caagaagcgc gttggctttg
     1741 ctcagcgctt catcctttcc gaatcgccca atccatcccg gcatcgctac gttttcggcg
     1801 gccgtgaact caggaagcag atgatggaac tggaaaacga agccgacgtt gtggttgcga
     1861 aagcgcgcca gctcgtcgtt gctcatcgaa gagacctcgg cgtctccaac gcgaatcgtg
     1921 ccgctgtccg gatgatcgag tccgccaagc aggtgcaaca gcgtcgattt gccaataccc
     1981 gaggggccga cgatggccag catctcgcca cgctccacgg tcagttccag cccactcaac
     2041 accgggacgg gaacttcggc gttgcgatag gttttcgaca gcgaccgcgc ttcgagaaag
     2101 ggcatgggag agattctatc tgcgtccatc cgcgtcatct gcggataaag ctttgtccgc
     2161 agatatacgc agatggacgc agatttctac ctcgcgatcg acgtcgactg cagcgctggc
     2221 gtcccgcccg ctggtgtgcc gctgccggac aaaccgacgg ttcccttcac gtggaaaacc
     2281 ggcatcttct gctgaagacc cttcaatgca aactcgccga gtgactcggt ttcgaagctg
     2341 cccatgatcc gatccatggt gttctgcgag acgacgacct gaccaggctt cgcaactccg
     2401 ctctccagcc gtgaggcgat gttcactgac gagccgagga cggtgtagtc gacacgcttc
     2461 tcggtgccga cgttgccgac gacggccagg ccgctgttga ttccgacgcg caccatcacc
     2521 ggcggcagct cggtctttgc acgttccacg ttccagtcgt tcaccaggcg ctgcaacatc
     2581 aggccggtga gtacggcgcg gtcggcatga tcatcctgcg gcagcggcgc gccgaagaac
     2641 gccatcaccg cgtcgccgat gaatttgtcg agcgttccgc cgtacgcgaa gatcgattcc
     2701 accgcgaacc cgaaaaagtg cgagagaaac tcggccactt cttccggttt cttcgactcc
     2761 gacatcggcg tgaagccgct gatgtccgcg aagaggatcg aaacatccgt gctgcgcacc
     2821 tccgtctccg tcgagatcga tcccttgacg atctcatcga cgacggacgg tgagtggtaa
     2881 cgctccatcc gcgaacggat tttcttctcc tcttcgatct tctccgcaag ctgtgctcgc
     2941 tcgatcgcca ccgccgcgaa gtttgccagc gcagtgagca gatcgagatc ctcttccgtg
     3001 aagctgccga tatgaatcgg cgaatcgacc tgcaccgcgc cgatgacgcg ctggcggttc
     3061 cacaacggca cgcacatcgc cgatcggatg ccgtgaatgg caatcgattt cccgccgagc
     3121 aaccgcgcgt cttcaagcgc gttcgaggtc atcaacgcca cttgctgctg cgacaccatc
     3181 ttcagaatcg tgcgcgagat ggggatgtcc tgctgctccg ccccttcgat gaacttcgtc
     3241 agtttcggaa cggggttgcc gtcttcgtcg aagaggatga tcagtccgcg ctcgaccggg
     3301 aggtagcgga agatgatgtc catcaccgtc gtcagcacga cttgcagctc ttccgacgcg
     3361 agcagcgcct ttgctacctg cacgagaacc tggaagatct tctcgcgcga cacggccggc
     3421 tcgcggattc ccggctgcga cggcgggcgc gtgaccggac ccttgtcggc caactccgcg
     3481 atgtcgagcc cgaactcgga attgaaatcg gagatgcggc ggatgacagt gcccgagggg
     3541 ttgtcgaaca tcgagtcggc gttgaagtcg acggagggaa gcgcgcggac atcgatgagc
     3601 actgaaccga tcgacagctt ctcgccggcc gcaacctgcg cctcggtgac gaggttgccg
     3661 ttcaccttca cgccgttcgt cgacttcaga tcgatcaccc accagtgatc gccgcgcagc
     3721 tcgaacttcg catgcttccg cgacacagac gggtgattca ggatcagatc attcccctcg
     3781 gtgcgcccaa ccgacagctc gtgcttgagg tccatgaccc gcggcacacc aagatcgacg
     3841 tagtgaagtt ggagctgctc cattcgagga gaggattata gcggtagggc gcggtgatcg
     3901 tcgggcatgt ggttccctac gaggcaggaa ggtccaacgc cttctctcgc caccgccgca
     3961 cactcattac gtcgaagagt tcaccttcga agacgacttg cttgaccgct ttcctgtcgg
     4021 cgcattctcg cttcaggtag gcgctcacat ccatccaccg gatctcgcca tctgacgtgc
     4081 ggatgaccag catgacgggg taggcgtgct gctgccagta gtcagcccag cgtggattct
     4141 tgatctggaa gacctccgcg ccatcgcgct tgcgcttttt cagataggaa tcgccggact
     4201 ttagttgcac gtagagacgc ttccccgagg cgcgactttg gtcatccttg aattcgatct
     4261 caccatcgat cccgtggtcg ctgttggtgt agccgcgata gatttggccg gattcagcca
     4321 cgatgaatcc cgtgtgatgc accgcctgca attcgcggct ctcgttgtcg atcaccgcct
     4381 gcccttcgta ctgcatcacg cgcactcgtt ccttcaccgc gcgcgagttg tacttctcct
     4441 ctatcgcatc gcgcaaaagg attggcttgc cgcaatcagg gcagaagacc tttcccttcc
     4501 cgccgggagc cagcgctttg tcaatcttcg tctggtcgat aaatgcctgt ccgaaggaat
     4561 cgcacttctt gttcccacaa gcatagtggc gcaggcgtat cacgctccgc gactgctgcg
     4621 tgaggtggtt gtgcacataa ctgtcaaaga gcacccggga gttcgcatca acatcgggtt
     4681 caaagtagac ttggagtcgt gaggcgccct cagtttcccg cgtcagcgtg aatcctagcg
     4741 ccgctccggt ctgcgttttg aaatcggccg ccgacttcca tagattcgtg ctctcaaaag
     4801 ccccggaatg atgcagacgc actaccaacg tggcgtacat gtcatccgtc ggaccctcga
     4861 agcgataagt aaccaacaca ctcgggtgac tgggctgctc tttccgctcg cgccggaagt
     4921 agctcggaaa ggtcagcatc gccgtgccat cgaaaggctc gcgtagacac catgcccggc
     4981 tgatgaacgt ctccagcagc gcgcgcaata cgacggcttc gtcttcgcgt gggagacgct
     5041 cgaaatcctg atagtccaaa ttggctgcga gcaattcgtc ctcgcggatg cagcccatct
     5101 cctgtggatg cttgcgcacc tttctcacta ctgcggcggc gtaacggctg agcacctccg
     5161 gccgcagaag gatgaagccg ccaaagtcga cgcgctggat catgccgggc ccggagagca
     5221 ggctcaccac cgcctcgagc tccccgagtt cgaaatcctc attacgcagc gtcatctcca
     5281 tgcgctgctt tagttcgctc aggcggatca acacgaggcc ttggtcgcgg agactgaggg
     5341 tttccagctt gattctatgg taaagggcgg gcgatgtcgt ctccggaatg ctcgtccaat
     5401 cgatggcctt cacgatggct ttgcgtagtg aatcgcaacc ctccccggtc ttcgcgctag
     5461 tcaggtgcag tggctggaga aagccgtgct cggccataaa tttcttcatg ttgttggcgc
     5521 taccaactag gccgccacga tcgacacgcc ctgccgcaag cagcttggcg aaaggtcttc
     5581 gggcagcctt ctgaaggtcg tggtcccatt ggccaagccc ctcgaaaggg ttttcgttct
     5641 gaggattgaa aacgagcact gctgccgccg tgtcatccat gaaaagctga tggacgagac
     5701 ggtaatctac ttgcccagca aaatcccaca accagatttc tcgctcgacg ccggctttct
     5761 tcttttcgtg atgcagcggc caatgggttg cccaagcccc gtcggttgaa ggaagaggct
     5821 tgtcatcttc gaccttgatg tcgtgggcga ggtagttgga aagaccgctc ttgccaacgc
     5881 cagaatcacc gacgaggaga actttggcgt ttgtgtattg gacctggtcc ggagcgggcg
     5941 gcaaagctgg cccagtatgt atcgttgccc gcgccacctt gccaaattcg gacagatccc
     6001 acacccgtat cccccccgcg taatcgccgg agaaagcgct gcgtccatca gggctgaacg
     6061 ccaaagttcg gacgtcgtcc gtatggccct tgagcacgcg cagacagagt cccgtctcca
     6121 cgtcccatag ccgaatggca ccgtcaaaag atcctgaaag agcatgacca cctttgctcc
     6181 atattaccgg tcgcacggat tgggagtggc ctttcattac gcccagacaa cgccccgttt
     6241 ccatatccca cagtcgaacg gtgctgtcat ttgaaccgga aagcccaaag cgcccgtcga
     6301 cgctgatgga cacatggtga acactttcgg tgtggccttc gagcacgtgt aggcagcgcc
     6361 cggtatggac atcccacaca cgaacgacgt tgtcttgacc gcccgtgagc gctcgacgcc
     6421 catctgcgct ccacgataac ccccaaatct cgctcggctg gcctttcatt ttgcgtaaac
     6481 atctcccagt ctcgacatcc cagagacgaa tagtcccgtc gtaagaaccg gagagcgcac
     6541 ggcgattgtc gatactccaa acgacgccca tgactctgcc agcgtggcct tctagcagac
     6601 ggagacagcg tcccgtctcg acttcccaaa tccgaatatc gttgtcgtcg ccaccggata
     6661 acgctcggcg ctgatcattg ctccacgcaa tgcaccaaac atcagccgag tggcctttca
     6721 ttacccgcag gcagcgccca gttcccacgt cccacagtcg aacggtgttg ccgccggcgc
     6781 cggtgagggc gcgccttcca tccggactga acccgtaggc caagatgcct ctttcttcgt
     6841 cgagttggat ggacttctca gcaagctgct cgcccacggc ttctacgtcc gctacctttg
     6901 gtttcgctgg cggtcggcat gcttcgagaa ggttcgcgta ttcctgcatg cggtccttcg
     6961 gcggccattt gacataaagg aattgcgcca gagacgcttt gactggcgaa tcgtcaagcc
     7021 gcaaaggaat aaagcgccgc tctttgttca gcggatcgcg aaaccggaac gtgccggcct
     7081 ctaattgcgc ccagtcggag ccgaacgcgt tcgccgacat gcagagtacg agcacgcgag
     7141 agtgctccag tccctcctcg atcttcgccg ggatgctgtc gccggggcgg atttcccagt
     7201 cgtcgagcca gacgcgcagt ccgtctgcac gcaggcgctc agcgagggcg cgaacgatcg
     7261 acttgtcctt ggagctatga ctgacgaaga cgtcaaatga gaatcgttca cccatgtaag
     7321 ggcctcacgc atctacctcg cgagtcgaag accgtcggcc acggcttatt gactctgtct
     7381 caaccgccaa gatgacattg agccaggtcc gggtcaacgt aattccttcg tgaaggatga
     7441 aggtctcgaa gtgcctacgc gatggcctgc ccctcgatat ccagctcatg gatcggcatc
     7501 ccgagtgcta ccagcggctc gcggatctgc gcggcgttgc cgacgatgac gacgatgacg
     7561 cgatcggggt cgatgtactt ctgcgcgacg tgctcgacgg cttctttcga gacggcgccg
     7621 atgttctcgc gatagcggtc gaagtagtcc tgaggcaggt cgttcagctc catgtccagc
     7681 acgcggccgg cgatgtcgct ggagctttgg acggtcgagg ggaacgagcc catgatgtag
     7741 ttcttcgtgt cttcgagctc gcgctcttcg acgtcgcccg tgcggatgcg gcgcaactcg
     7801 gacagcacct cggttaccga ttcgcgcgtg acctcgttgc ggactggagc gctgacgacg
     7861 aaggggcccg cctgacgccg aaacgcgaag accgaccgcg cgccgtacgt gtaaccatgc
     7921 ttctcgcgca ggttgaggtt gatgcgcgaa ttgaacacgc cgccgaggag cgcattcatc
     7981 accgacagcg cgaaatagtc ctcggtgctg cgtgcgattc cgatgtggcc gactcggatc
     8041 tcggactgca cggccgtggg acggtcgatc aggtaaatgc ggcttgcttc gttcgccttc
     8101 ggcgagatct taggccgagg gggaacttcg actacaggcc agtcggcgaa gaagcgtgtc
     8161 aatccaccca gggccttctc ggcatcgacg tctcctgcta cgacgatcga actgttgttc
     8221 ggaacgaagt gggtggcgta gtagcggcga acatcatcga ctttgatgtt cgcgacggat
     8281 tcgggattgc cgatgaccgt gttgccgtac gtgccgcggc cgtagatgag gtgcgagaag
     8341 cgcttgccgg cgatcgcgcc gggatcgtct ttctgctgga gcaactccat gagccgctca
     8401 ctacgcacgc gatccagcga cgactctgga aggatcggcc ggcgattcac atcggcaaag
     8461 atggccagcg cttcatcgaa cgtccgtgag agcgaatcga ccgagacgta cgacgcgtcc
     8521 cagtcggcgc cgctgccgag ggacgcgccg aggaggccga ggtcctcggc gagacggaca
     8581 gcgtcacggg aaccggcgcc ttcatcgaga agctcggatg ttagggacgc caggcctgcc
     8641 agttcgggtg tgtcgtagtc ggcacccgag cgcaccagcg tgcgcatcga gaccagcggc
     8701 gcattgtgat tctccgtcac cagcaggcgc aggccgttgt ccagagtgcg gcggacgacg
     8761 ttagggaaat ggtacggacg cggcgcgccg ggctgcggcg cggtcgtgcg gtcgatcgtc
     8821 gtcatgcgtt ctccgggacg aacgtgctgg tcacgcggtt atcgggcacg aggtactccc
     8881 gcgtcacgcg ctgcaggtca gcgctggttg cggcgcggta cggatcgagc cacgtgtgaa
     8941 ccagcgaggg atcatcaaaa taggtcgcca tcatcccgat cagatcggcg cgcgagtcgt
     9001 agttttcgat ctgatgcgcg tactcgacct cggcgcgatt cttcgcacgc gtcagctcat
     9061 cggcagtgat gccgtccgac acgacacgca cgatctcggc gtcaatggcg cgctcgacgt
     9121 cttcgattgg aacgtcgtcg ttcgccgtcg cctgaatcat caggatgccg gtcgcctcgg
     9181 tggggagcac gtacgcagcg acatcggtgg cgatctgctg ctcgaggacg agagctttct
     9241 ccagccggct ggctttgtcg ccgctgagca ccgacgacaa cagatcgccc gcgatccagt
     9301 cagggtgacc catcttcgga aggtgataga gacggtagac acggggtagc tgcaccggcg
     9361 aaggaactgt ttcgcggcgc tcaccgttca gcggcttccg cgtcagtgcg aacttcggaa
     9421 aagaccctcc cgccggaatc gaaccaaagt agtgctcgac caactccttc gcttcttcgg
     9481 gtacgaaatc gccgatcagc gtgagcgtgg cgttgtcggg acggtaccag gtgctgaaaa
     9541 actcgcggat gtcatcgagc gatgcagcgc cgaggtcttc catcgagccg atggtgggcc
     9601 agtgatagga gtaatcggga tcgtacgcca gattgagcag gcgctcgaag gccgtgccgt
     9661 agggcacatt gtcgtagcgc tggcggcgct cttccttcac cacctcgcgc tggatgtcga
     9721 gcttctcctg cgtcagagcc ggaaggaaga agcccatccg gtcggactca agccaaagac
     9781 cgagggccag gtagttcgac ggcagcgtct cgaagtaatt cgtacggtcg aagaacgtcg
     9841 tgccgttcag cacgccgccg atcgactgca cgtaccggaa gtgctcgttg ttgccgacgt
     9901 gctccgagcc ggagaaaagc atgtgctcga acaggtgcgc gaacccagtg cgcccgggac
     9961 gctcgttctt cgagccgacg tggtaccaga ggttgactgc gacgagcgga gcggagtggt
    10021 cttcgttgag gacgacccgg aggccgttgt cgagagtata ggtttcaatc gaaagcatcg
    10081 gcggcgggtt gtatcacgat cggaagggca gaaggcagag agcagaagga tgaagtttgg
    10141 accgaagccg cggaggcgaa ccaacttcgt ccttctgcct tctgccttct gccttatcct
    10201 tccagccgtg ccgccgctgc cgtttcgtca tatcgccgtc gagggacc
//
LOCUS       DATLVW010000553         7086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_602352, whole genome shotgun sequence.
ACCESSION   DATLVW010000553 DATLVW010000000
VERSION     DATLVW010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602352"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VNN25_16510"
     CDS             <1..175
                     /locus_tag="VNN25_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HXH93187.1"
                     /translation="LYAHPPDASGKTIVDFNKAYNPPCAFTPYATCPLPPPQNRLPIR
                     VEAGEKKYAGGHG"
     gene            complement(245..1654)
                     /locus_tag="VNN25_16515"
     CDS             complement(245..1654)
                     /locus_tag="VNN25_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXH93188.1"
                     /translation="MNSEQVAKNVGQIWDDSIVPTLQHYIRIPNQSPLFDPEWKKNGH
                     MDRAVALARAWVESLQIPGLSIETHELDGRTPLIFIEVDGDDSSTVLMYGHLDKQPAM
                     VGWNEGLGPWTPVLRDGKLFGRGGADDGYAIFAAVSAIQAVKQQGAPHSRIVIIIECC
                     EESGSVDLPAYIDLLSEKIGTPRLVICLDSGCGNYSQLWMTTSLRGSLVGNLTVEVLT
                     EGVHSGDASGIVPSSFRILRILLDRIEDAATGRIIPEWLHAEVPADRLAEATETARIL
                     GDEIHDKFPFLPGMKPMGNDPLELLLNRTWRPMLSYTGQAGLPDLVQGGNVLRPKTSL
                     KLSLRIPPTLDASNLDRKLQELLESNPPYGAHVTFEPEKGAGGWEAPKVAAWLEQSVD
                     SASQAFFGKPATTWGEGGSIPFMGMLGQRYPNAQFLITGVLGPHSNAHGPNEFLHIST
                     GKNVTACVAQVIADERNAK"
     gene            1897..2463
                     /locus_tag="VNN25_16520"
     CDS             1897..2463
                     /locus_tag="VNN25_16520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH93189.1"
                     /translation="MPTAAPAAPAGEEVPPPTLPVLEPPQEDIGELYVRHRTLLMHIG
                     SRKFRIAESEAEGLIQEVFLSFMTTTTKISNVRSWLVAAMCNASRHYWRVQGRMESLP
                     ENYDDHTDPISDDIAEEIARVITMQQALDYLQPRCRETLYLHYYQGLSANEVARAQDT
                     TSRYAEKLIHNCLKRVREIYFSITAVKR"
     gene            2460..3818
                     /locus_tag="VNN25_16525"
     CDS             2460..3818
                     /locus_tag="VNN25_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93190.1"
                     /translation="MMIEQHYDEEVLAGFLDEPIDAAARDKHLDTCSLCKRTLQSIRD
                     TTALLKKPAIWDRQRFSTTAKPETLAFLRGMQQTMAEENAAAQGYVTLLLAGDRESWA
                     ASLAAHPEWRTPGLVRQLIAATDRAIDLMPPDALEITRLAIDVAEQLAESDCVVRLRG
                     NAWYERGYALLYTGAFHEALRALKTAELQFAQLPIANHEIGRLNLVRGMIYRALERFD
                     EALPLIAGAVAFFRQYGDIERCHSASLALGIMLSSTKRFREALAVHLEIANDKAVTPR
                     CYAAALQNAAICHRELGEIDSAIDCFVKAISAAETVGMISFRAKTRWTLARILVEQQQ
                     YKSALAILTELRDEFEVLGMTNDVAVIALDIAELLLAAGRNSEVADACKQAIKYFEKM
                     HLTSSQGALTAVTYLCEAAVVGNATAALVSDLRVAFFSEASGRSLHARPYESLPQSTD
                     RG"
     gene            4144..5055
                     /locus_tag="VNN25_16530"
     CDS             4144..5055
                     /locus_tag="VNN25_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH93191.1"
                     /translation="MSVVLSVRDVVKTFGAVRAVDGVSFTVRRGTITGLLGRNGAGKT
                     TTIRMITGVFFPDAGTIEWLGSERDARDRIGYLPEERGLYRQMKVKEHLLFLAEIKGC
                     RGTAVAQSAESWLKRFELWDKRDAKVETLSKGNQQKVQLIGTLLHDPDLIILDEPQSG
                     LDPVNMLLVRNLLRELKDEGRTILLSTHMMAEAERMADEIILVHKGKVVLSGAIDTVR
                     SSFGKNTLHIDYEGDGSFLENLPEVRRAMIVNNAAELSLKEGADPQRVLEATVGRLRV
                     RKFEIASPSLEEIFIEQVGAETMEGIR"
     gene            5052..6377
                     /locus_tag="VNN25_16535"
     CDS             5052..6377
                     /locus_tag="VNN25_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93192.1"
                     /translation="MNKMFAVIKREYLQAVRRKMFMFMTVFFPVLMAALFILPGLLIS
                     KGLGDKKVAVVDGTGGLRSAFVHHIAPEVPDPKKALGGGRRAELPQSLDVEYADAHGQ
                     SNLDAAAKPYLDRLNVDKSSDGRLDAVLLIPANAFEGNDAKMTFYSRSSTDLFSQERL
                     GSIANRAIQRQRLTGRGIDPTIVDTLTREVPVSSVQLSKSGEQKKGSEANFIIGFIFA
                     ALLIIPSFIYGLETMRGIVQEKSERIVEVLVSSMSPRELLTGKILGVAGVGLTQVSVW
                     LIAIAAAGTFGAASAMAAGFNVTQFLRPMVFVYFALFFILGYLTFVCVYAIAGAACNT
                     DREAQQLVAPIQMVMMLPWFVMFAIITNPDSTFAVVLSLMPVYGPMTMFVRTLVSEPP
                     VWHVVVSIAVSIVTIFAFFYVTAKIFRVGILSYGKRPTIPELWRWMKVA"
     gene            6465..>7086
                     /locus_tag="VNN25_16540"
     CDS             6465..>7086
                     /locus_tag="VNN25_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXH93193.1"
                     /translation="MEIQTLNDIYTVTCTLPRAAAMKFKRDGKWVDTGVDELRDTVRY
                     FATGLHLLGLKEGDRVAILSENRPEWAMADFAILGSAAICVPIYPTLLGWQVEYILND
                     SGAVAVICSTEEQMKKVAGVRDHCPHVHNVIVCDPPPTLPAGVLSFAQVVESGRKGDD
                     AAARARFDDVRGRAKTSDVATLVYTSGTTGNPKGAMLTHGNIASNVQ"
BASE COUNT         1416 a         2150 c         2117 g         1403 t
ORIGIN      
        1 cctctacgcg catccccccg atgcgagcgg gaagacgatc gtcgatttca acaaggcgta
       61 caacccgcct tgcgcgttca cgccctacgc gacgtgtccg ctgccacctc cgcaaaaccg
      121 gctaccgatc cgcgtcgaag caggggagaa gaagtacgcg ggtgggcacg ggtagcggtc
      181 gcgcggctag cggcgtcgct gcgactttcg caacgcggtc gctactatac gacggcgccg
      241 atcgctactt cgcgtttcgc tcgtccgcga tcacctgtgc cacgcatgcc gtcacattct
      301 tgccagtgct gatgtgaagg aattcatttg gcccgtgggc attgctgtga gggccgagca
      361 cgccggtaat caggaactgc gcgtttggat aacgctgccc cagcatcccc atgaacggaa
      421 tcgagccgcc ttcgccccat gtggtggctg gctttccgaa gaacgcctga gatgcggagt
      481 cgaccgactg ctcgagccac gcggcgacct tgggcgcttc ccatccgccg gctccctttt
      541 ccggttcgaa ggtcacgtgc gcgccgtacg gaggattgct ttcgagtaac tcctgtaact
      601 tgcggtcgag attcgaggcg tcgagcgtcg gagggatgcg cagggacagc ttgagcgagg
      661 ttttcggccg aagcacgttg ccgccctgaa cgagatcggg aagacccgcc tggcctgtgt
      721 acgagagcat cggccgccac gtcctgttga gcaggagctc gagcggatcg ttgcccattg
      781 gcttcatgcc gggaaggaac ggaaacttgt catgaatctc atcgcctagg attcgggcgg
      841 tttcagtggc ttcggcgaga cggtcggccg gaacctcggc gtgcagccat tccgggatga
      901 tccggccggt ggccgcgtct tcgatgcggt ccagaaggat gcgaaggatg cgaaaactcg
      961 aaggcacgat cccgctggca tcgccagaat gcaccccttc ggtgagcact tctaccgtga
     1021 gattgccgac gagcgagccg cgtaacgaag tcgtcatcca gagctggctg tagttgccac
     1081 aaccgctgtc cagacagatg acgaggcgcg gcgtgccgat cttttcggat agcaggtcga
     1141 tgtacgcggg caaatcgacc gagccgcttt cttcgcagca ttcgatgatg atgacgatgc
     1201 gcgagtgcgg cgcgccctgc tgcttgaccg cctggatcgc cgagacagcg gcgaagatgg
     1261 cgtagccgtc gtccgcgccg ccgcggccga aaagctttcc gtcacggaga acgggagtcc
     1321 acgggcccag accctcattc catccgacca tggccggctg tttgtcgaga tgcccgtaca
     1381 tcagcacggt cgaggagtcg tcgccatcga cttcgatgaa gatcagcggc gtgcgaccgt
     1441 cgagctcatg ggtctcgatg ctaaggccgg ggatctgtag cgactcgacc caggccctgg
     1501 ccagcgcgac ggcccggtcc atgtgaccgt tcttcttcca ctccggatcg aagaggggag
     1561 actggtttgg gatgcggatg taatgctgta gtgtgggaac gatcgagtcg tcccagatct
     1621 ggccgacatt cttcgcgacc tgttcagaat tcatggagta ccttccgtcg gctcggtttg
     1681 cgacggcgga ggatagcatc actcgaaccg acccgaacgg gtgggggctg gaatcgatga
     1741 aaaaaccgac gtttccaaaa ttaccgacac ctttccgcta ctacatcggg tatgctgtac
     1801 agactccaaa aaagtccgag ggaatttgat gtcgggaagt aacccctcta acgagggaag
     1861 agggacgcga ttgtgttcga tcccaatatc ctgaaaatgc caaccgctgc gccggccgcc
     1921 cccgcaggag aagaggttcc acctccgact ctgccggtcc tggagccacc acaggaagat
     1981 atcggtgagc tttacgtgcg gcaccggacg ctgctgatgc acatcggcag ccggaagttc
     2041 cgcatcgcgg agtcggaagc ggaaggtctc atccaggaag tgtttctttc gttcatgacc
     2101 acgaccacga agatctcgaa cgtgagatcg tggctggtcg cggcgatgtg caacgccagc
     2161 aggcactact ggcgcgtcca gggacgcatg gagagcctgc ccgagaatta cgacgatcac
     2221 accgatccaa tctccgacga catcgccgaa gagatcgcca gagtgatcac gatgcagcag
     2281 gcactcgatt accttcagcc tcgctgccgc gaaacgcttt acctccatta ctaccaggga
     2341 ctctcggcga atgaagtggc gcgggcgcag gatacgacgt caaggtacgc agagaaactg
     2401 atccacaact gtttgaaacg tgtccgcgag atctacttca gcatcacggc ggtgaaacga
     2461 tgatgatcga gcagcactac gacgaagaag tgctcgccgg atttctcgac gaaccgatcg
     2521 acgccgcggc gcgtgacaag catctggaca cctgttcgct ctgcaagcga acgctccagt
     2581 cgatccgcga cacgaccgct cttttgaaga agccggcaat atgggaccgg cagagattct
     2641 caaccaccgc aaagccggag acgctggctt tcctgcgagg aatgcagcag acgatggcag
     2701 aagagaacgc ggcggcgcag gggtatgtca ccctcctgct cgcgggcgat cgcgaatcgt
     2761 gggccgccag cctcgccgca caccccgagt ggcggacacc aggcttggta cgacagttga
     2821 tcgcggcgac ggaccgcgcg atcgatctga tgcccccgga tgccctcgag atcacgcgcc
     2881 tggcaatcga tgtcgccgag cagttagcgg aatcggattg cgtggtacgg ctccgcggga
     2941 acgcgtggta cgagcggggg tatgcgctgc tctacacggg cgcttttcac gaagcgttgc
     3001 gcgctctcaa gaccgcagag ttacagtttg ctcaacttcc gattgccaat catgaaatcg
     3061 gccggcttaa tctggtgcgc ggaatgatct atcgcgctct cgagcgcttc gacgaagcgc
     3121 tgcctctcat cgccggggca gtggctttct ttcgccagta tggcgatatc gagaggtgcc
     3181 actctgcgag ccttgctctg ggcattatgc tatcgagtac caagcgtttc cgggaagctc
     3241 tcgctgttca tctggaaatc gcgaatgaca aagcagttac tccccgatgt tatgccgccg
     3301 ctcttcagaa tgccgcgatc tgccaccgcg aacttggcga gatcgactcg gcgatcgatt
     3361 gctttgtgaa ggcgataagt gcggccgaga ccgttgggat gatttcattc cgagccaaaa
     3421 cgcggtggac gctcgcgaga attcttgttg agcaacagca atacaaatcc gctctagcaa
     3481 ttcttaccga gcttcgtgac gagttcgaag ttcttggcat gaccaatgat gtcgcggtga
     3541 tcgcactcga tatcgccgag ttgcttctcg ctgcgggaag gaacagcgaa gtcgccgacg
     3601 cttgcaagca ggccatcaag tatttcgaga aaatgcacct cacgtctagc cagggcgcgc
     3661 tgaccgcagt cacgtatttg tgtgaagctg cggtcgttgg aaacgccact gccgcgcttg
     3721 tctcggatct tcgcgtggcg ttcttttccg aggcaagtgg ccgttccttg catgctcggc
     3781 cctacgaatc tttgccccaa tcgacggatc gggggtaggg aaaagcccag cccagacact
     3841 ccttaatagg ggcgccctcc aaaataaccg ggttcatcag agctcgcgga gggtatatgt
     3901 ctacacgatc tactgttcgg gttgccactg ttgtacttgt cgttgcacta accggtagca
     3961 ttgccacggc cgctccaagt cgcgacggtg gttctgacgg ctttctcgcc ggcatcggca
     4021 agattgtcag gcagattggc cggatactgc gtccacttga cgagcctggc tggcccaaac
     4081 cctgacgggg ctactggctc ccgccataca atacgccggc ccagtcagcc gtggagtgtg
     4141 ctcatgtcgg tcgtgttgtc ggttcgggat gtcgtgaaga cttttggcgc cgtgcgggcg
     4201 gtggacggag tctcgttcac cgtccggcgc gggacgatta ccggtttgct cggccggaac
     4261 ggcgcgggaa agacgaccac gatccggatg atcaccggcg tcttttttcc cgatgccgga
     4321 acgatcgagt ggctcggcag cgaacgtgat gcccgcgacc gcatcggcta tcttccggaa
     4381 gagcgtggcc tctaccggca gatgaaggtc aaggagcacc tgctctttct cgctgagatc
     4441 aaaggttgcc gtgggactgc ggtcgcgcaa tcggcggaga gctggctcaa gcgctttgag
     4501 ctttgggaca agcgggacgc caaggtggag acgctgtcga agggcaacca gcaaaaggtc
     4561 cagctcatcg gaacccttct gcacgatccc gatctcatca ttctcgacga gccccaatcg
     4621 ggcctcgatc ccgtcaacat gcttctggtc cgcaaccttc ttcgcgagct gaaagacgaa
     4681 ggccgtacga tcctgctctc cacgcacatg atggctgagg cggagaggat ggcagacgag
     4741 atcatcctgg tgcacaaggg gaaggtcgtt ctctccggcg cgattgacac ggtgcgcagc
     4801 tccttcggga agaacacttt gcacatcgac tacgaagggg atggctcctt tctcgaaaac
     4861 cttcctgagg tcaggcgcgc catgatcgtc aacaacgcgg cggagctgag cctcaaggaa
     4921 ggtgccgacc cgcagcgcgt gctcgaagcc accgtcggcc gcctccgagt acgcaagttc
     4981 gaaatcgcct cgccatcgct tgaagagatc ttcatcgaac aggtcggcgc cgagaccatg
     5041 gagggcattc gatgaacaag atgttcgcgg ttatcaagcg cgagtacctc caggcagttc
     5101 gtcggaagat gttcatgttc atgacggtct tcttccccgt tctaatggcg gcacttttca
     5161 tccttcccgg actcctgatc tccaaaggtc tcggcgacaa gaaggttgcg gtcgtcgatg
     5221 gtaccggcgg acttcgtagt gccttcgtgc atcacatcgc gcccgaagtt cccgatccaa
     5281 agaaagcgct cggcggaggc aggcgcgccg agctccctca gtcgctcgat gtcgagtacg
     5341 ccgacgccca cgggcaaagc aatctcgacg ccgctgccaa accctatctc gaccgcctca
     5401 atgtcgataa gagctccgac ggccgcctcg acgcagtcct tctcattccc gcaaatgcct
     5461 tcgaaggcaa cgacgcgaag atgacgttct acagccgctc ctcgaccgat ctgttctccc
     5521 aggaacgact cggatcgatc gctaaccgcg caatccagcg acagcgcctc accgggcggg
     5581 ggatcgatcc aacgattgtt gacacgctca cgcgcgaagt cccggtaagc agcgtgcagc
     5641 tttccaagag cggcgaacaa aagaaaggta gcgaagccaa cttcatcatc ggcttcattt
     5701 tcgcggcact cctcatcatc ccttccttca tctacggcct cgaaacgatg cgcggcatcg
     5761 tgcaggaaaa gagcgagcgc atcgtcgaag ttctcgtctc gtcgatgtcc ccgcgcgagc
     5821 ttctgaccgg caagatcctc ggcgtggctg gcgtcgggct cacgcaggtc agtgtgtggc
     5881 tcatcgccat cgccgcagcg ggaacgttcg gagcggcaag cgcaatggct gccggtttca
     5941 acgtcacgca attcctccgg ccgatggtct tcgtctactt cgcgctcttc ttcatcctcg
     6001 gctatctcac atttgtctgc gtttacgcca tcgccggcgc cgcttgcaat accgaccggg
     6061 aagcccagca gctcgtggcc cctatccaga tggtgatgat gcttccgtgg ttcgtgatgt
     6121 ttgcgatcat caccaacccc gactccacct tcgccgtcgt gctctcgctc atgcctgtct
     6181 acgggccgat gactatgttc gtgcggacgc tcgtttcgga accgccggtc tggcacgtgg
     6241 ttgtttcgat cgccgtctcc atcgtgacga tcttcgcatt cttttacgtg acggcgaaaa
     6301 tcttccgcgt gggcatcctc tcgtacggca agcggccgac gattccggag ctgtggcgat
     6361 ggatgaaggt agcctgatga aacggggctg aaccgtggaa caccgccggc cgcgagcggc
     6421 tgctatgatc cgcgcctagt ccacaagaca gggggcccac gcggatggag atccagactc
     6481 tcaacgacat ctacaccgtc acttgcacgt tgccccgtgc ggccgcgatg aagttcaaac
     6541 gcgacggaaa atgggtcgac accggcgtgg atgagctccg cgacacggtt cgttatttcg
     6601 ccaccggatt gcatctgctc gggctaaagg aaggcgatcg cgtggccatc ctctccgaga
     6661 atcgtccgga gtgggccatg gcggacttcg ccatcctcgg cagcgccgcg atctgcgttc
     6721 ctatctaccc gacgctgctc ggctggcagg tcgagtacat cctcaatgac tccggcgccg
     6781 tcgccgtcat ctgctcgacc gaagagcaga tgaagaaggt cgcgggggtc cgcgatcatt
     6841 gcccacacgt tcacaacgtg atcgtttgcg atccgccccc gaccctgccg gcaggtgtgc
     6901 tcagcttcgc gcaagtcgtc gagagtggtc gtaaaggcga cgacgctgcc gcccgtgccc
     6961 gtttcgacga cgttcgcgga cgagcgaaga ccagcgacgt ggcgactctc gtttacacct
     7021 cgggtaccac aggcaatccg aagggagcca tgctcaccca cggcaacatc gccagcaacg
     7081 tccagg
//
LOCUS       DATLVW010000554         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_434785, whole genome shotgun sequence.
ACCESSION   DATLVW010000554 DATLVW010000000
VERSION     DATLVW010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434785"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..2957
                     /locus_tag="VNN25_16545"
     CDS             24..2957
                     /locus_tag="VNN25_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93194.1"
                     /translation="MLGPIQRKTAEAELLSMISGLTPTRADDREGEGRSDRTAERRAR
                     DLASLARRSVTAFRRRIALAPELETPAWAQPAAGRVLIAPLLAGKWDETKEADQAMVS
                     KLAGMSYPALEEHLVRWSHESDAPVRHVGNFWFIVAKEDAWPLLHRLITREDLRRLTE
                     VVTTVLGTVDPRFDLPEDKQWMARAIGHAPLYSDALRHGLADTLALLGTDGALTTPTA
                     GESAEAVAHSAVARLLENANADWRLWASLSHLLPLLAEAAPDQFLAAVDTGVRGDAPV
                     LREMFREGADAFTSSSPHTGLLWALERVGWSPEYLPRAAVAIGRLAVLDPGGKLANRP
                     AASLRQLFLLWHPQTNADLEARLRVLDTLRERVPTEAWRLMNRLLPQPHDMVMNHQPA
                     TWRTWGQEETPVTHAEYARGVHEIARRMLHDVDSDGARWCDLIEHISRLPQEDEKAVL
                     DGLAALDLSALAPGACVAIWHVLRKIISRHRSHPDANWSMPTDRIDRLAELHTRFTPT
                     DVEERFAWLFGNHPALLDGREPDWDAHEKVLSDHRRDAVATIFAEGGIDALMHFASVV
                     EQPNHVGIALSGLPQSDAITEELLARYLASPTLFERGFARGFAWASVRKFGHEWAEDL
                     LRRRGESMRPNQRAELIDCLPYEPYTFDLVDAADEETQDAYWKRMYAFGIPDGLGEYG
                     ARRLLKHGRPYAALDFVAARFHLNKKELPPAVLIADVLEAAIHAKPSDIDPLDQSLGY
                     DVGELLDKLVDSPGDIDRSRIASLEWAFMPAIRDHHHPRLLHDELARDPAFFVEIASI
                     VYRGKGEEPRDLSEQDSMQARVAYDVLDTWHTIPGRQGNTIDALELGEWLREARRLLA
                     EKNRAAVGDRLIGQLFAASPTGDDGAWPHPAIRDVIEDLGSTEIERGISMRIYNNRGV
                     VTKNLDEGGEQERQLVDEYERWANLVNNKWPRTAAMLRQVASSYQHEATRSDIDVELR
                     DHLDD"
     gene            complement(2996..3745)
                     /locus_tag="VNN25_16550"
     CDS             complement(2996..3745)
                     /locus_tag="VNN25_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93195.1"
                     /translation="MAAMPFLGADWIRDDRVRAWVEVAGFVLGVLGFAIALMHVRHLE
                     RATQSISTRFLGPFPRFLPQLVRLLLRAQDVEILCDYVAYGALVAPKQAREYEAALKN
                     STGARKKNVRLTVMNDERLRTYTPKFVADEKQPREVRPERRLDVSNFLQSVAGMPRDD
                     AAHANVATFIEARIANEHATLARLKETVHDPQALDDEFSVYYWIFDQERAIFSFPALS
                     RDAIEYGFETSDQALIAALSDLGQRLRTPRP"
     gene            4136..4498
                     /locus_tag="VNN25_16555"
     CDS             4136..4498
                     /locus_tag="VNN25_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93196.1"
                     /translation="MPKTLPTHSAKPLSGAELRTLISQHLPAWQQACDADDGESIVLH
                     EASFGNSTDELFLFACAIKYAAAKGKHVHVATGTGSSKHEPETMLPSATLGSVYREVA
                     TKKRHAPTRRSSNRGKGK"
     gene            4987..>5350
                     /locus_tag="VNN25_16560"
     CDS             4987..>5350
                     /locus_tag="VNN25_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93197.1"
                     /translation="MEKNQDVRALVAAVVILALIVLGVHYYRAHARMEEEAQTETENR
                     QAQSLADRPQTKQFLQIYFYSHTDCSATIEPNQEIGQAELDRLLDGREFPGTADTSTV
                     RSSWCSNGTGWIGVVKLPS"
BASE COUNT         1140 a         1633 c         1515 g         1062 t
ORIGIN      
        1 attcagcatc ccagaaaacg gtcgtgctcg ggccgattca gagaaagacc gctgaagcgg
       61 agttgctgtc catgatcagc ggactgactc ctacgcgagc ggatgaccgc gaaggagaag
      121 gccgttccga tcgcaccgcc gagcggcgag cgcgtgacct cgccagcctt gcacggcgca
      181 gcgtgacagc atttcgccgc cgcatagcgc ttgcacctga acttgaaacg cccgcttggg
      241 cgcaacccgc cgctggtcgg gttctcatcg cgccgttgct cgctggcaaa tgggacgaaa
      301 cgaaagaagc tgaccaagcg atggtcagca aacttgccgg catgagctac ccagctcttg
      361 aagaacatct ggtccgctgg tcccacgaat cggacgcgcc ggttcgccat gtcggaaact
      421 tttggtttat cgtcgcgaag gaggatgctt ggccgctcct ccaccggctg ataactcgtg
      481 aagatcttcg tcgtctgacc gaagtagtaa caacggtttt aggcactgtt gatccgcggt
      541 tcgatctccc tgaagacaag caatggatgg cgcgcgccat cgggcatgca cctttgtact
      601 cggatgcgct gcgtcacggt ctggctgata ctctcgccct tcttggaact gatggcgcgc
      661 tcaccacgcc gaccgcaggc gaatcggcgg aagctgtagc ccactctgcg gttgcgcgtc
      721 tccttgaaaa cgcgaacgca gactggcggc tttgggcgag tctgtctcac ctcttgccgc
      781 ttctcgcaga agcggctccg gaccaatttc tcgccgcggt tgataccgga gtgcggggag
      841 atgcgcccgt actacgcgag atgtttcgag agggcgctga tgctttcacg tcgtcgtcgc
      901 cgcacaccgg ccttctatgg gcgttggagc gggtcggatg gtcaccagaa tacctcccgc
      961 gcgctgcggt tgcgatcggc cgtctggccg tcctcgatcc aggagggaaa ctggcaaatc
     1021 gacctgcggc aagtcttcgc cagctatttc tgctctggca tccgcaaacg aacgctgatc
     1081 tcgaagcgcg actgcgcgta ctcgatacgt tgcgcgaacg cgttccaacc gaagcttggc
     1141 gcttgatgaa cagacttctt ccccaacccc acgacatggt catgaatcat cagcccgcga
     1201 cctggcgtac ctggggacag gaagaaactc cggtgacgca tgccgagtat gctcgcggcg
     1261 tccacgagat cgcccggcgg atgctccatg atgtcgacag cgatggcgcg cgctggtgcg
     1321 atctcatcga gcatatttcc cgcctgccgc aagaagatga gaaggctgtg ctcgatggcc
     1381 tggccgcctt ggatctctcg gcactggcgc ccggcgcgtg cgtagcgatt tggcatgtgt
     1441 tgcggaagat catttcccgg caccgcagcc atccggatgc caactggtca atgccgaccg
     1501 atcgcatcga ccgcctcgca gagcttcata cccgcttcac gcccaccgat gtcgaagagc
     1561 gtttcgcatg gctcttcggc aaccatcccg ctctcctcga cggccgcgag cccgattggg
     1621 acgcacacga gaaggttctg agtgaccatc ggcgcgacgc cgtcgccacc atcttcgctg
     1681 aaggcggtat tgacgctctg atgcactttg caagcgtcgt cgagcaacca aatcacgtgg
     1741 gcattgcgct cagcggcctt cctcagtcgg atgccatcac ggaagaactt ctcgcccgct
     1801 accttgcctc gcctacgctt ttcgagcgcg gtttcgctcg cggcttcgct tgggcatcgg
     1861 tccgcaagtt cggacatgaa tgggccgaag acctcctacg caggcgtggc gaatccatgc
     1921 ggccgaatca gcgcgcagaa ctcattgact gcttaccgta tgagccgtac acgttcgatc
     1981 tcgtagacgc ggccgatgaa gagactcagg atgcgtactg gaaacgcatg tacgccttcg
     2041 gtatacctga tgggcttggc gagtacggcg cgcggcgatt gctgaagcat gggcgcccct
     2101 acgccgcgct cgattttgtc gcagcgcggt ttcatctaaa caagaaagaa ctgcctcccg
     2161 cagtgctcat cgccgatgtc ctcgaggctg ccattcatgc caagccgagt gacatcgacc
     2221 cgttggatca gagcctcggc tacgacgtag gcgaattgct cgataagctc gttgactcac
     2281 ccggcgatat tgaccgcagt cgcatcgcgt cgctcgaatg ggcgttcatg cctgccattc
     2341 gagaccatca ccatccccgg ctgcttcacg acgaactcgc tcgtgatcca gcgttctttg
     2401 tcgagatcgc atccatagtc taccgtggca agggcgagga accaagggac ctttccgaac
     2461 aagactcgat gcaagcgcgc gtcgcctacg acgttctcga cacgtggcac acgattcctg
     2521 gtcgacaggg caacacgatc gatgcgcttg agctaggcga atggctccgc gaagcacgac
     2581 gactgctcgc ggagaagaat cgcgcggcgg ttggcgaccg tctcatcggc caactctttg
     2641 ccgcgtcacc taccggtgac gacggcgctt ggccccaccc cgctattcgg gatgtgattg
     2701 aggacctcgg gagcacagaa atcgagcgag gtatctcgat gcgcatctac aacaatcgcg
     2761 gcgtggtcac taagaatctc gatgaaggcg gagagcaaga gcgccaactc gttgacgaat
     2821 atgaacgttg ggcgaacctc gtaaacaaca aatggccccg aacggccgca atgcttcgtc
     2881 aagttgcctc ctcgtatcaa cacgaagcta cgcgatcaga tatcgacgtt gaactgcgcg
     2941 accatctcga cgattgacgt acaccaagga aactttatgc agcggtccac gagattcagg
     3001 gccgtggggt tcgcaatcgc tgacctaggt ctgacagcgc agcaatcagc gcctggtctg
     3061 acgtttcgaa cccgtattca atggcgtccc gagacaatgc cggaaacgag aagatcgccc
     3121 gctcttggtc gaagatccag taatagaccg agaactcgtc atccaaggcc tgtggatcgt
     3181 ggaccgtttc cttgaggcgc gcgagggtgg catgctcgtt tgcgattctc gcttcgatga
     3241 atgtcgcgac gttcgcatgc gcagcatcgt cacgcggcat cccggcgaca gattgcagga
     3301 agtttgaaac gtccagacgg cgctcgggac gcacttcgcg tggctgcttc tcgtcggcaa
     3361 cgaactttgg cgtgtatgtt ctcaatcgct catcattcat gaccgtcagt cgtacgttct
     3421 tcttccgagc gcccgtagaa ttcttaagcg ccgcctcgta ctccctcgcc tgctttggcg
     3481 caacaagtgc gccgtacgcg acgtaatcgc agagaatctc gacatcctgg gcgcgcaaca
     3541 atagacgcac cagctgcgga aggaatcgag gaaatggccc aaggaatcgg gtagagatgg
     3601 attgcgtcgc gcgctccagg tgtcttacgt gcattagcgc aattgcaaaa ccaagaacac
     3661 ccaaaacgaa tccagcgact tcgacccacg cacgaacacg atcgtcgcga atccagtccg
     3721 caccgagaaa cggcatcgcc gccattaccg cgaacagcgc tacgacaagg acgacaatcg
     3781 tgcgggccga cgggagattg tgtctagtgg atcgcgacgg catcggtgaa cttgctcggc
     3841 actcgcagcg gatctgctat ttactctggg ttattgccac gacggcgtac tcgaatgaga
     3901 aagcaacacg gtgtctcatc ggacgaacgt tcgtttgaaa tcgtcaattc ctcggcaagc
     3961 cacgtacggt tgcagttgct cgtgagaatc tcaattcgcg cgccggacgc ctggcaatgg
     4021 tgctctgcaa ccgagtctcc aacggcgctg ccccattctg ctctctcctt tgacgccgcc
     4081 ctcccgcgct tctatgatta cggagtgttc atacagataa acactccttc ctctcgtgcc
     4141 taaaaccctc cccacccact ccgcgaaacc cctttcgggg gcggaactgc gaacgctaat
     4201 ctctcaacac ctgccggcct ggcagcaggc ctgcgacgcc gatgacggcg agtccatcgt
     4261 gctccatgaa gcctccttcg gcaattcgac cgatgagctc ttcttgtttg cgtgcgcgat
     4321 caagtatgcg gcggccaagg gcaagcacgt gcatgtggca acaggaaccg gaagctcgaa
     4381 acacgagccc gaaacgatgc tcccttccgc cacgctcgga tcggtctata gggaagtcgc
     4441 cactaagaag cgtcacgcac ccactcgccg gagttcaaac cgcggaaagg gaaagtagcg
     4501 ccgattatag gtcgcacatg gcattgcacg tcgtacttga cagaagtata ccgttatgct
     4561 aatattaggc gtaccttgaa aagctgctag ctataaccct gacggctctg cctgggcgct
     4621 cttgcacgtt gcagttgaag cgctcggcca aaggcccgaa tcgcgcccga caccaacgcg
     4681 cgtttccctc tagacctacc cacgctccta acaagcgggc atcgcgactg ccgtggtcgc
     4741 ctgcgttcgc gccgcatgtg atgcgttcgg cgaatactgg gtgctttcaa tggccatggt
     4801 gccgtgtcgc taagcactcc ccgcactcca attcctctaa agggcgttcg cgattcgcac
     4861 gcgcaagacg atctcaacaa ggagaatggg tactccagga gctgtgaggg ttatagctag
     4921 ccaatcaaga accgcctgag agtcaggcct acttgaaaag cacaacaaca atgatggagg
     4981 agttccatgg agaaaaacca ggatgtaagg gcgttggtag cagcagtggt gatccttgcg
     5041 ctcatcgtgt tgggagtcca ttactaccgg gctcatgcca ggatggaaga agaggcccaa
     5101 acggagaccg agaaccgtca agcccagtcc ctcgctgaca gaccacagac caagcagttc
     5161 ctccagatct atttctacag ccacactgac tgctcggcga cgattgagcc aaatcaggag
     5221 atcggccaag cggaactcga cagacttctc gacggccgcg agttcccagg cacggcggac
     5281 acttctacgg ttcgatcgag ctggtgcagc aacggcaccg gatggattgg cgtggtgaag
     5341 ctcccttccg
//
LOCUS       DATLVW010000555         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_233581, whole genome shotgun sequence.
ACCESSION   DATLVW010000555 DATLVW010000000
VERSION     DATLVW010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233581"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1238)
                     /locus_tag="VNN25_16565"
     CDS             complement(195..1238)
                     /locus_tag="VNN25_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93198.1"
                     /translation="MKRLLVSLALAFFATAAFAQAADHDVLVTLDGTVYTIEQQFPSA
                     SSGIAATNFLDLSIQNGSATQHTLVPESLSAGYHFGGALAYDADSKTLFVLWIHMPSG
                     MSSELLLASYRDGKWQPAVSIDDQGYNARKNLRLGITRRVSQLQKDGSYADVPALLLH
                     AVWWEETGHGEEARYAMLPIDNGALSPSAIEVHSLEEFVSTTNEPFNAVAANFNAEIL
                     RHPAIVSSPMQSEVDVVFGDTKTNTIHGVTLHPIADTRVHIPVGIGGGGPGVGKPMSL
                     AAPPSFSSNWKGPISVIERGDRLAFANAGETSLNYITYANGTWSDVKSIAIDSRFPVE
                     AALAAVDKMVSTQ"
     gene            complement(1235..2116)
                     /locus_tag="VNN25_16570"
     CDS             complement(1235..2116)
                     /locus_tag="VNN25_16570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93199.1"
                     /translation="MSDLNSRIEELRQDALAKVRSEEYDEALALYDEALTLATDEEVC
                     ELITINKGHALIAAERTGPEVKALPMILMRRRNAHHTFLASYALLYTHRLTNELKRAI
                     FYGQIALDAANQADQTFWKLAALNELGIVYESDSQFGKAIEHFEQALTLIATLDDASQ
                     QQFSRVAICQNLGSSRVIIGDTVEGIRMIESIIDQVQVRSTLSDSYIDLCYGYLDLED
                     YAKARHLGETGLELACEPRQVRNAHYLLGEAAYKMGDVDAAEQHFEHLARFYPQFRHL
                     KSLLFAIDLRSMLNLKL"
     gene            complement(2109..2474)
                     /locus_tag="VNN25_16575"
     CDS             complement(2109..2474)
                     /locus_tag="VNN25_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH93200.1"
                     /translation="MNKPAQLELVGRKIRQLRRQRKLTQVELADRIGVHQSDLSRMEQ
                     GEYKVGLDTLLKILGTFDLSIGDFFDEGARTETVVQKYRSLSPTAQKEVESFIEFKRA
                     QEEDGSWDSDGGESEGDDV"
     gene            2370..>2892
                     /locus_tag="VNN25_16580"
     CDS             2370..>2892
                     /locus_tag="VNN25_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93201.1"
                     /translation="MHADTIGKLYLRQLTLTPELPDFAADKLELGWLIHWSFVDFYAA
                     ETINSKPIYGLTARLSSPEDPKTFKNAHLLRELVKRDFHARFSGSVLGILWAVLQPLS
                     LVILYWFVFTYMFSRGPAAGNGHYIEFLIAGLLPWLGLNEGIVRSTTSIVDNAPMVRR
                     LAFRSELLILVPHT"
BASE COUNT          538 a          834 c          889 g          631 t
ORIGIN      
        1 ggcggtgccg gaacgctgct ccggcgtctg agaagcgtcg aatggatgct gcgaggactg
       61 tcggcgccgc gcctggtctc tgcgacacgg tcccgcgccg ttgaagcttg ctcgcatctc
      121 tcgggacagc cgaaaacaaa aagagccggc gaacatcgcc ggctcttcgc atcatggctc
      181 gttagggagg attactactg cgtgctgacc atcttgtcga ccgccgccag ggctgcttcg
      241 accgggaacc ggctatcgat agcgatcgac ttgacgtcgc tccacgtccc gttcgcgtag
      301 gtgatgtagt tgagcgacgt ttctccggcg ttggcgaacg cgagacggtc gccgcgctcg
      361 atgacgctga ttggtccttt ccagttggag ctgaagctgg gtggcgccgc gaggctcata
      421 ggcttgccaa cgccaggtcc gccaccgccg attccaaccg gaatgtgcac ccgcgtatca
      481 gcgattggat ggagcgttac accgtggatc gtgttggtct tcgtgtcacc gaagacgaca
      541 tcgacctcgc tctgcatcgg cgagctgacg atggcgggat ggcggaggat ttccgcgttg
      601 aagtttgcag cgactgcgtt gaacggctca ttggttgtgg aaacgaattc ttcgagcgaa
      661 tgaacttcga tggctgatgg agagagagca ccattgtcga tgggcagcat cgcatagcgc
      721 gcttcttcgc cgtgcccggt ctcttcccac cagaccgcgt ggagcaggag tgccggcacg
      781 tcggcgtacg agccatcctt ctgaagttgc gagacgcggc gggtgatgcc gaggcgtaga
      841 ttcttgcgag cgttgtaacc ctgatcgtcg atcgaaaccg caggttgcca cttgccgtcc
      901 cggtaggagg cgagaagcag ttcactcgac atgccgctgg gcatgtgaat ccagagaacg
      961 aagagggtct ttgaatccgc gtcataggcg agagcgccgc cgaagtggta accggccgac
     1021 aacgactcgg ggaccagcgt gtgctgcgtt gctgagccgt tttgaatcga cagatccagg
     1081 aaattcgttg ccgcgatacc cgatgatgcc gagggaaact gttgctcgat ggtgtacacg
     1141 gtcccgtcga gtgtgacgag cacatcatgg tctgcagcct gcgcgaaggc ggcggtggcg
     1201 aagaatgcga gggcgagcga gaccaggaga cgtttcatag ttttaggttg agcatcgacc
     1261 gaagatcgat cgcaaacaga agactcttca gatggcggaa ttgcgggtag aagcgggcga
     1321 gatgttcgaa gtgctgctcc gccgcgtcga cgtcacccat cttgtaggcg gcttcgccga
     1381 ggaggtaatg cgcgtttcgg acttgacggg gctcgcacgc gagctcgagg ccggtctcac
     1441 cgaggtgacg tgcctttgcg tagtcctcca agtcgagata gccgtagcag aggtcgatgt
     1501 acgaatcgga gagcgtgctc cggacctgga cttgatcgat gatgctctcg atcattcgaa
     1561 tgccttcgac cgtgtcgccg atgatcacac ggctggaacc gaggttctgg cagatagcga
     1621 cccgcgagaa ctgctgctgc gatgcatcat cgagggttgc gatcagcgtc agggcctgct
     1681 cgaagtgctc aatcgccttc ccgaactgcg agtcgctctc gtagacgatg ccgagctcgt
     1741 tgagcgcggc cagcttccag aaagtctgat cggcttgatt tgccgcatcg agcgcgattt
     1801 gcccgtagaa gatggcgcgc tttaactcgt ttgtcagccg atgcgtgtac agcaacgcgt
     1861 acgacgccag gaatgtgtgg tgtgcgttgc ggcggcgcat caagatcatc ggaagggctt
     1921 tcacttccgg gccggtgcgc tcggccgcga tcagagcgtg acctttgttg atcgtgatga
     1981 gctcgcagac ttcttcatcc gtggcaagcg tgagcgcctc gtcgtatagc gctagcgcct
     2041 cgtcatattc ttccgaacga accttcgcca gggcgtcctg gcgtagttcc tcgatgcggc
     2101 tgttcaggtc agacatcgtc accctcgctc tccccaccgt ctgaatccca cgagccgtct
     2161 tcttcctggg cccgcttgaa ctcgatgaag ctctccactt ccttctgcgc tgtcgggctg
     2221 aggctgcggt acttctgaac cacggtctcg gtccgtgccc cttcgtcgaa gaagtcgccg
     2281 atactcaggt caaatgttcc gaggatcttc agcagggtgt cgaggccgac tttgtactct
     2341 ccctgctcca tccgcgagag atcggattgg tgcacgccga tacgatcggc aagctctacc
     2401 tgcgtcaact tacgctgacg ccggagctgc ctgattttgc ggccgacaag ctcgagctgg
     2461 gctggcttat tcattggagt ttcgttgact tctatgctgc tgagaccata aactctaagc
     2521 caatctacgg gcttaccgcc cggctgtcaa gtccagagga cccgaaaacg ttcaagaacg
     2581 cccacctcct ccgcgagctt gtcaaaagag actttcacgc gcggttctca ggttcggtcc
     2641 tcggcatcct ctgggctgtc ctccagcccc tgagcctcgt catcctttat tggtttgttt
     2701 tcacgtacat gttcagccgc ggtccggccg cggggaacgg ccactacatc gaattcctga
     2761 tcgccggcct gttgccatgg cttggactga atgaaggcat cgtccgatcg acgacgagca
     2821 tcgtcgacaa cgcaccgatg gtgcgccgtc tggcgtttcg tagcgagctc ctgattctgg
     2881 tcccgcacac ga
//
LOCUS       DATLVW010000556         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_358378, whole genome shotgun sequence.
ACCESSION   DATLVW010000556 DATLVW010000000
VERSION     DATLVW010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358378"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VNN25_16585"
     CDS             <1..687
                     /locus_tag="VNN25_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93202.1"
                     /translation="HSLLRYIRPNIFVDRQTDLDGNVIYRRLSPGIFVLGAKNLQANA
                     VLLFKNQTRVGSDLLEETYLNWFVQLDPSRRFPRITFNGRAGDMIDFANGRVGKGSSL
                     GLAATVRPLDRLTFEPNISHEWLNINNGRLYTADVEYLKTTYSFSSKALVRIIGQYVT
                     TNRNPSLYGFPVSPHSGSFLGSVLYSYKINWQTVLFLGYGDDRTITPSNDLVKLDRSL
                     FFKVSYAIQK"
     gene            1008..1238
                     /locus_tag="VNN25_16590"
     CDS             1008..1238
                     /locus_tag="VNN25_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93203.1"
                     /translation="MNETKHFAICIDNGEYAGTLELRKVYEILEDATAASRNHVRVID
                     ESGEDYLYPSSWFVSVAVPENVEQRLHDLVVK"
     gene            1380..2279
                     /locus_tag="VNN25_16595"
     CDS             1380..2279
                     /locus_tag="VNN25_16595"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HXH93204.1"
                     /translation="MRRLLLIAILVAVALPVTAAEPNPSVRQRELAEKLLVAMNVGGN
                     SSAMMDAMFAQIEKQVVGAAGGPGKDADDIAEAKDLFAAFRERARKLDIAGLLREAQV
                     RIYARHFTESELADLNAFYATPTGQKSIQVMPQLIAEGMQAGIDNVSPKIEALMAEVT
                     AEQEMKRPWRRTMIDMRTVATAIEAYQTDQEDGTYPAGDYAGLKAVLQGDHAYLTRFP
                     EKDMWGNPYAYVVSEDRHHYRIISSGADSNFEWDSRRITPAKPGETITMRYSDRLEDD
                     LIYADGSFIQLPTQAKPKTKKEN"
     gene            complement(2307..>4138)
                     /locus_tag="VNN25_16600"
     CDS             complement(2307..>4138)
                     /locus_tag="VNN25_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HXH93205.1"
                     /translation="RELQPAQLSADATTMQPGHSPLTLSGMIVGTPRYMAPEQILGAP
                     VDGRTDLFSAATLLYEMVAGAPPFANQNPMQLFHALVYENAPNLAGSAAISAVNRVIH
                     RAMAKKPVDRYQSADAMAQDLRETTLISDAAAPIVAHKVTRLIVLPFRMLRADAEIDF
                     LAHAMPEAITTSLAAIRSLIIRSPMAAAKFASEAIDLKRLATEADVDVVVTGTLLRAG
                     EQIRVTAQLVEVPGGTVLSSVSSQATMSGIFELQDLLTQRIIDSLELPRVEQSSGAMR
                     RDVPATPLAHEFYLRAGQQGESPSAWLIARDLYTRSVEEDPHYAPAWARLARIYLLIG
                     KYCNDSVTNYALAESAAKHALEINPELPIAHQAYAHLEVSTGRSRDAMLRLLDRVARG
                     SNDPNVFAGLVTALRFCGLLEESCAAHEQARQLDPTISTSAAHSYWMIGRYEEALAAV
                     DVARDFGDAAFIYESMGRLDDALAVFDDRIQRLQTAGGAGTSSLNFRVFETFRSALLG
                     KPDALDLFDQFVKFPDPEGLYYMGRGYARVGGLDQALTMLDLAERGGFFCYPFFMRDP
                     WIDPLRGDARLNEILHRAEAKWRDAKTAFENHPGSRVLTIGAK"
BASE COUNT          809 a         1240 c         1318 g          771 t
ORIGIN      
        1 cacagcctcc tccgttacat ccgcccgaat atcttcgtcg atcggcagac cgacctcgat
       61 ggcaacgtca tctatcgccg cctctcgccc ggcatttttg tgctcggcgc gaagaacctc
      121 caggcgaacg ccgtcctgct cttcaagaat caaacgcgcg tgggcagcga tctgctggaa
      181 gagacgtatc tcaactggtt cgtgcagctc gatccgtcgc ggcgctttcc gcggatcacg
      241 ttcaacggcc gcgccggcga catgatcgat ttcgccaacg gccgggtagg gaagggctca
      301 tccctcggcc ttgccgcgac ggttcgcccg ctcgaccggc tcaccttcga gccgaacatc
      361 agccacgaat ggctcaacat caacaacggc cggctctaca ccgccgacgt tgaatatctc
      421 aaaacgacgt acagcttttc ctcgaaggcg ctcgtgcgga tcatcggcca gtacgtcacg
      481 acgaaccgca acccatcgct ctacggtttc ccagtctcgc cgcacagtgg ctcattcctc
      541 ggatccgtcc tctacagcta caagatcaac tggcaaaccg tcctcttcct cggatacggc
      601 gacgaccgga cgatcacacc gagcaacgat ctcgtgaagc tcgatcggtc gctgttcttc
      661 aaggtttcgt atgcgataca gaagtaggtg gcgtgcagcc cgatcgtatc gtggcgacgt
      721 ttccggctga ttgcacgaca cccctcatcc gccttcggca ccttctcccc tcgaaaagca
      781 cgaggggaga aggcgctcga ttggaggttg gcgcgagagc gtttgagagg acctcggtgc
      841 gtgtctgtgt ctttgtctgt ctctgtctct ctcaagtcga ttgatggcag ctcactaaat
      901 tcgagcgcct tctccccatc gcttttcaga tggggagaag gtggccgaag gccggatgag
      961 gggcgcgtgc ctgctaaact tcgttcaagc gatatctcag ttggccaatg aacgagacaa
     1021 agcatttcgc aatctgcatc gacaacggcg aatacgccgg cacgctcgag cttcggaagg
     1081 tgtacgagat tctcgaagat gcgacggcgg cctcgcgaaa ccacgtgcgc gtgatcgacg
     1141 aatccggcga ggattacctc tacccgagct cctggttcgt cagcgtggct gttcccgaaa
     1201 acgtcgaaca gcggttgcac gatctcgtcg tcaaataggc gcgaccccgg tcgtcagcgt
     1261 ctgacctgat cacgttgggc cggctcggga agcatggaat cgcgggtatc attcaggcac
     1321 cgcggcttgg ggttccttcg ttggttatcc aagccgaaaa gtgatccagg agactcacca
     1381 tgcgtcgttt gctgctcatc gcgatcctcg tcgctgttgc tttgccggtg actgccgctg
     1441 agccgaatcc atcggtgcgg caaagggagt tggccgagaa gctgctcgtc gcgatgaacg
     1501 ttggcggcaa cagcagcgcg atgatggacg cgatgttcgc gcagatcgag aagcaggtcg
     1561 ttggcgctgc cgggggtccc ggcaaggatg ctgatgacat cgccgaagcg aaggaccttt
     1621 tcgccgcctt ccgggagcgc gctcgcaagc tcgacattgc cggtctgttg cgcgaggcgc
     1681 aagttcggat ttacgcgaga cacttcacgg agagcgagct tgccgacctc aatgccttct
     1741 acgcgactcc caccggccag aagtcgatcc aggttatgcc acagttgatc gccgagggaa
     1801 tgcaggccgg gattgacaac gtgtcgccga agatcgaggc gctcatggcc gaagtcaccg
     1861 cagagcagga aatgaaacgg ccgtggcgaa ggacgatgat cgatatgcga acggttgcca
     1921 ccgcaatcga ggcgtatcag acggaccagg aagatggaac ctacccggca ggcgactacg
     1981 cagggttgaa agccgtgctc cagggcgacc atgcatacct gaccagattt cccgagaagg
     2041 atatgtgggg aaacccgtat gcgtacgttg tctccgagga tcggcatcac tatcgaatca
     2101 tcagcagcgg cgctgactcg aacttcgagt gggacagccg gcggatcaca ccggccaagc
     2161 cgggcgaaac gatcacgatg cgttacagcg atcgcctcga agacgatctg atttacgccg
     2221 acggctcttt catccagctc cccacgcagg ccaagccgaa gacaaagaag gagaactgat
     2281 cgtccgcggt gcgacccctc ggttgatcac ttcgcgccga tcgtcaacac gcggcttcca
     2341 gggtgattct cgaatgctgt ctttgcgtcg cgccatttcg cctcggcgcg atggaggatc
     2401 tcgttgagtc gggcgtcgcc gcgcagcgga tcgatccagg ggtcgcgcat gaagaacggg
     2461 tagcagaaga atccgccgcg ctcggcgaga tcgagcatcg tcagagcttg atcgagccct
     2521 cccacgcggg cgtaaccgcg gcccatgtaa tagagcccct cgggatcggg gaacttgacg
     2581 aactgatcga acagatcgag agcgtcgggc ttgccgagga gcgcagagcg gaaggtttcg
     2641 aatacgcgaa aattcagtga tgacgtgccg gcgccgcccg ctgtctggag acgctggatg
     2701 cgatcgtcga acacggccaa cgcatcgtcg agtctgccca tcgactcgta gatgaacgcg
     2761 gcgtcgccaa agtcgcgagc gacgtcgacc gctgcgagcg cctcttcgta tcgaccgatc
     2821 atccagtagg aatgcgcggc gctcgtggag atggtcggat cgagctggcg ggcctgttcg
     2881 tgggccgcgc acgactcctc gagaagtccg cagaagcgca gcgccgtcac cagtcccgcg
     2941 aaaacattcg ggtcgttgct gccgcgtgcg acacggtcga ggagacgcag catcgcatcg
     3001 cgcgagcgcc ccgtcgacac ttccagatgc gcgtatgcct gatgtgcaat cggcagctcg
     3061 ggattgatct ccaacgcgtg ctttgccgcc gactccgcga gcgcgtagtt tgtgacgctg
     3121 tcgttgcagt acttgccgat gagcaagtag atgcgtgcaa ggcgcgccca ggctggcgcg
     3181 tagtgcggat cttcttcgac ggatcgggtg tagagatcgc gagcgatcag ccacgcactg
     3241 ggcgactccc cctgctgtcc ggcgcgcagg tagaactcgt gcgcgagcgg tgtggcggga
     3301 acgtcgcggc gcatcgcgcc cgatgactgc tccacgcgcg gcagctccag tgagtcgatg
     3361 atccgctgcg tcagcaaatc ctgcagctcg aagatgccgg acatcgttgc ctgcgacgat
     3421 accgacgaca gcaccgttcc tccgggtact tcgacgagct gcgcggtgac acgaatctgt
     3481 tcgccggcgc gaaggagcgt gcctgtgacg acgacgtcga cgtcggcctc ggtggccagc
     3541 cgcttgaggt cgatcgcctc ggacgcaaac ttcgccgcgg ccatcggcga gcggatgatg
     3601 agcgagcgga ttgcggccag cgacgtggtg atcgcttccg gcatggcgtg cgcgaggaag
     3661 tcgatctccg catcggcgcg aagcatgcgg aagggaagga cgatgagccg cgtgactttg
     3721 tgcgcgacga tgggcgccgc ggcgtctgag atcagggtcg tctcgcgaag atcctgggcc
     3781 atggcatcgg cggattggta tcgatccacc ggcttcttgg ccatggcgcg atgaatcacg
     3841 cgattcacgg cggagatcgc agcggagccg gcgaggttcg gcgcgttttc ataaacgagc
     3901 gcgtggaaga gttgcatcgg attctggttc gcgaacggcg gcgcgccggc aaccatctcg
     3961 tacagcaagg tcgcggcgga gaagagatcg gtgcggccgt cgaccggcgc gccgaggatt
     4021 tgttcgggcg ccatgtagcg cggagtgccg acgatcatgc cggagagtgt gagcggcgag
     4081 tggccgggct gcatcgttgt cgcatcggcg gagagttgtg ccggctgcag ctcgcgcg
//
LOCUS       DATLVW010000557         3030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_358431, whole genome shotgun sequence.
ACCESSION   DATLVW010000557 DATLVW010000000
VERSION     DATLVW010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358431"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VNN25_16605"
     CDS             complement(<1..618)
                     /locus_tag="VNN25_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXH93206.1"
                     /translation="MTTPDQTETVRTQFTRESIVDDYRIAFRSRQASLIGRREVLTGK
                     AKFGIFGDGKEVAQLAMARAFRKGDWRSGYYRDQTFIFASGIGTLDEFFAQLYAHADT
                     GHDPWSAGRSMNCHFGSRILNDDGSFRNQTDAYNTSADVSPTGSQMPRLVGLAYASKL
                     YRELEELKAMTQFSRNGDEIAWGTIGNASCAEGMFWESVNAAGVLQ"
     gene            complement(975..2225)
                     /locus_tag="VNN25_16610"
     CDS             complement(975..2225)
                     /locus_tag="VNN25_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93207.1"
                     /translation="MLPDENAKAWRFARYAIIAIWIAAMARLFIADVWDETNALVVFG
                     RGSGQSAMQLIRVILKTPLPFWRPIPTILVALAIHWIPDPEVTWRLLRGVNVVIVLAA
                     LWLFLKTLDAWDGRSERRNTFFTLAYLFSGGAIIVATWFANIFDAAVMLLVAWGLLLV
                     TRNKFVSAGLLFGVAFFFKETAAMTLPLLVLLIAVDRVKIRDAVKVAIPTIALGVIYF
                     ILRSLVVPFGSAADTHQFHAATILPTVMGLIESYWRESLWGHATILGYVFFLFSLGAM
                     RGWRSRVVFAGFVAFAALIYMEMFTTYQGNALIHYLMFIPRLYFIPVVLSLFVFAVDR
                     RWWGVAILAIPLLAGAVGTYMRYERFQRSYRNLYRYARTAPKPLRIDYAMKPLHDPAR
                     GIEIGDFPDAHVRIDPVNGLLVKR"
     gene            2305..>3030
                     /locus_tag="VNN25_16615"
     CDS             2305..>3030
                     /locus_tag="VNN25_16615"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXH93208.1"
                     /translation="MSPRVLLRVLRVHHWTKNLLVFVPLLMAHDVNDMSRVVNALLIF
                     VSFCFASSATYIINDVIDAKHDRLHRSKRNRPISANEISARAALLIVPFAVAGSLAIA
                     ASINREAAFGIVLYLLLSTLYTLVVKRVMLADVILLATFYSLRVLIGGAATGIVVSRW
                     LLAFSSFLFISLALAKRYADLTRAVSNDEEAPGGRSYRTGDREVLLSLGTSSGLISVL
                     VFALYLNSAQVTQLYSKTDPLWLI"
BASE COUNT          590 a          920 c          981 g          539 t
ORIGIN      
        1 ctgcaggacg cccgccgcgt tgaccgactc ccagaacatc ccctcggcgc aacttgcatt
       61 gccgatggtg ccccaggcga tctcatcgcc gttgcgcgag aactgcgtca tcgccttcaa
      121 ctcctcgagc tcgcgataga gcttcgaggc ataagcgagc ccgacgaggc gcggcatctg
      181 cgagccggtc ggcgaaacgt ccgccgaggt gttgtaggcg tcggtctgat tgcggaagga
      241 gccgtcgtcg ttcaggatcc gcgaaccgaa gtggcagttc atcgaccggc cggcggacca
      301 gggatcgtgg ccggtgtcgg cgtgtgcata caactgcgcg aaaaactcgt cgagggtccc
      361 gatccccgag gcaaagatga aggtctgatc gcgatagtag ccgctgcgcc agtcgccttt
      421 gcggaacgcg cgggccatgg ccagttgcgc gacctctttg ccgtcaccga agatgccgaa
      481 cttcgccttg ccggtcagca cctcgcggcg gccgatgagg ctggcctggc gcgagcgaaa
      541 cgcgatgcgg tagtcatcga cgatgctctc gcgcgtgaac tgcgtccgga cggtctcggt
      601 ctgatctgga gttgtcacga gggaagttta actgagagag gcagagacag gagacaagag
      661 atcagagatc aggtgggaga cgccaccctg atctcttgtc tctgatgcct ctgatctcct
      721 gatctctgat cctgatctct gagagctatc agggccgtat ccaaccaccg aggcgggacg
      781 cctcgatggc cgcaggcgag acgcccgcac tcctcagcgc ttcgaaagga gtgcggtcgt
      841 ctcgaccgcg ggcactcggg cgtcctcgcc cgagtgcggt cagggcggag gtcgaaatcc
      901 atcgacgtct tgcttgcggt gaagtacggc ctcgtagttt ccgccgcgca aactcccagg
      961 gcacccgcgc gaacttatct cttcaccagc aagccgttca cgggatcgat ccgtacgtgt
     1021 gcgtcgggga agtcgccgat ctcgattccg cgggcggggt cgtgcagagg cttcatcgcg
     1081 tagtcgatgc ggagcggctt cggggctgtt cgggcgtaac gatagagatt gcggtagctg
     1141 cgctgaaaac gctcgtagcg catgtacgtt ccgacggcac cggcgagtag cgggatcgcc
     1201 aggatcgcca cgccccacca gcggcggtcg acggcgaaca cgaagagcga caagaccact
     1261 gggatgaaat acagccgcgg gatgaacatc aggtagtgaa tgagcgcgtt gccctggtag
     1321 gtggtgaaca tctccatgta gatgagcgcg gcgaacgcca caaacccggc gaagacaaca
     1381 cgggagcgcc aaccgcgcat ggcgccgagc gagaagagga agaaaacgta gccgagaatc
     1441 gtggcgtggc cccagaggct ctcgcgccag tagctctcga tcagtcccat gactgtcgga
     1501 aggatcgtcg cggcatgaaa ctggtgcgtg tcggccgcag agccgaacgg aacgactagg
     1561 ctgcgcagga tgaagtaaat gacgccgagg gcgatcgtgg ggatggctac tttgacggcg
     1621 tcgcgaatct tgacgcgatc gacggcgatg agcagcacca gcaacggcag cgtcatcgct
     1681 gcggtctcct taaagaagaa ggccacgccg aagagcagcc ccgcgctcac gaatttgttg
     1741 cgcgtcacca gcagcaatcc ccaggcgacg agcagcatca ccgcggcgtc gaagatgttc
     1801 gcgaaccagg tggcgacgat gatcgcgccg ccggagaaga ggtaggcgag cgtgaagaaa
     1861 gtgttgcggc gttcggagcg gccgtcccag gcatcgagcg ttttcagaaa cagccagagc
     1921 gcggcgagga cgatcacaac attgactccc cgcagcagtc tccacgtcac ctcggggtcc
     1981 ggaatccaat gaatagccag cgcgacgagg atggtcggaa tcggacgcca gaacggtagc
     2041 ggcgttttca gaatgacgcg gatgagctgc atcgcgctct gtccgctgcc gcgcccgaag
     2101 acgacgagcg cgtttgtctc atcccagacg tcggcgatga aaagccgcgc catcgcggcg
     2161 atccagatgg cgatgatggc gtatcgggcg aagcgccacg ccttcgcatt ttcgtcgggc
     2221 agcatcgatg cgcggagtct atacgacgca aacggcccgc gaattgaact ggacgcccga
     2281 aaggcagtca cataaggtgt ccgcgtgagc ccgcgggttc tgcttcgcgt ccttcgcgtc
     2341 catcactgga cgaagaacct gctcgtgttc gtgccgcttc tgatggcgca cgacgtcaac
     2401 gacatgagcc gcgtcgtgaa tgcgctgctg atcttcgtca gcttctgctt cgcgtcgtcg
     2461 gccacctaca tcatcaatga cgtgatcgac gcgaagcacg accggctgca tcgcagcaaa
     2521 cgcaatcgcc cgatcagcgc gaacgagatc tcggcgcgcg cggccctgtt gatcgtgccg
     2581 ttcgccgtgg ctggcagtct ggccatcgcg gcatcgatca atcgcgaggc ggccttcggc
     2641 atcgttctct atctcctcct ctcaacgctc tacacactgg tggtgaagcg cgtcatgctc
     2701 gcggacgtga ttctgctggc gacgttctac tcgctgcggg tgctcatcgg cggcgcggca
     2761 accggcatcg tcgtctcgcg gtggctgctg gcgttttcga gtttcctgtt catcagcctg
     2821 gcgctggcga agcgttacgc agatctgacg cgcgcggtct cgaatgatga ggaggcaccc
     2881 ggcggccgca gctaccgcac cggcgaccgc gaagtcctcc tgagcctggg cacgtcgagc
     2941 ggcctgatct ccgtgctggt gtttgcgctt tatctcaaca gcgcgcaggt gacgcagctc
     3001 tattcgaaga ccgatccgct ctggctgatc
//
LOCUS       DATLVW010000558         3450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_434896, whole genome shotgun sequence.
ACCESSION   DATLVW010000558 DATLVW010000000
VERSION     DATLVW010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434896"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1601
                     /locus_tag="VNN25_16620"
     CDS             87..1601
                     /locus_tag="VNN25_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH93209.1"
                     /translation="MIKTEQALSFEERVRIAAELLESLDRDRGQLVQVSAEERQRLLH
                     ATRRVAEPDHRARRKLVKASVRERKAARLKQDDQVLNSAGIRELRRKPVYSTPNSYRE
                     ESPQEDPRILTESQHCYVCKQHYLTVHHFYDQLCPACGDLNFAKRTELADLRGRVALL
                     TGGRVKIGYQAGLKLLRAGARLIVTTRFPRDSAARYAREDDFGEWAERLEIFGLDLRH
                     TPSVEAFCHEMLTTRDRLDFIINNACQTVRRPPAFYEHMMESETAALHTLSDAERHLL
                     GVFEGLRGYRMLPEASASIKTMKLEEVTGLTHSAQLSQVPLLPGDHERDLFPEGRLDQ
                     DLQQVDLRGRNSWRLLMAEVSSVELLEVQLVNAVAPFIINARLKPLMLRTANRDKHIV
                     NVSAVEGQFYRNQKTTRHPHTNMAKAALNMMTRTAAADYYTDGIHMNSVDTGWVTDED
                     PAALAERKTAVHRFHPPLDIVDGAARILDPIITGINTGEHVWGQFLKDYKPTDW"
     gene            complement(1694..3337)
                     /locus_tag="VNN25_16625"
     CDS             complement(1694..3337)
                     /locus_tag="VNN25_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93210.1"
                     /translation="MLLRHVASIVCAGLIVFPISSSGQNYGTRINVTAIEVSATVRDA
                     NGNLPSDLKPGDFELIEDGKRQRIIGVTYSHGASAVTTATKESIGTTPSKPQLPWQVV
                     IYLQQSLSATEGLRLAIKSLIPQVDRLVAMGDVEVITDDPSTHSLLGPTRDAAALRSL
                     FETLGNEARGREKLIRIRQEFMTGDRNTSDKDDRLTRRSLMRKAARQESTILRAQQDA
                     MFGWLSRYARHASGDSMRTLVFVTDGFDLAPAGFYSIDRGDIRPDLPTELRELNASIH
                     QNEIAQALAAQGWTVLSYATSPIETRVTHFAAATHEPLVPHSFNVRENVGHTIINKDP
                     LAPLKLLADATGGSVLTDPSEIGVRLDALDQRVVITYQVDRPSDDKLRHVEIRAVRAG
                     LTINAQKWVGTRSTLAAMPVMAELLATELATSTTRGEDVDRGELPVTCSIKLGSLAKG
                     VRTSEIEVRVNVAPIEGELASLESGTLRFTVAVVVPHENPFTSTNRLDQLDFSSKQGF
                     ATTITVRHRDGAKMAVIAEEPSTGAWGGCTIGGDRGSFP"
BASE COUNT          658 a         1109 c         1025 g          658 t
ORIGIN      
        1 ctccagtctc ccacccccca ctccctactc cctactcccc ccaacccgga tttcccgtgc
       61 gccacccgat gtttcgggcg acgctgatga taaaaaccga acaagcgctt tccttcgagg
      121 agcgggtccg catcgccgcc gaactgctcg aatcgctcga ccgcgaccgt ggacagctcg
      181 tgcaggtcag cgcggaggag cgtcagcgcc tgcttcacgc aacgcggcgc gtcgccgagc
      241 ccgatcatcg cgcgcgacgc aagctggtga aggccagcgt gcgcgagcgc aaggccgcgc
      301 gcctaaagca ggacgatcag gtgctcaact ccgccgggat tcgcgagctt cggcgcaagc
      361 cggtctactc cacgccgaat tcgtatcgcg aagaatcgcc gcaggaagat ccgcgcatcc
      421 tcaccgaatc gcagcattgc tatgtctgca agcagcacta tctgaccgtc caccatttct
      481 acgatcagtt gtgtcccgcc tgcggcgatc tcaatttcgc aaagcgtacg gagctggcgg
      541 acttgcgcgg acgcgtcgcg ctgctgaccg gtggccgcgt caagatcggt tatcaggctg
      601 gtctcaagct gcttcgcgcc ggtgcgcggc tcatcgtcac gacgcgcttc ccgcgcgact
      661 cggcggcgcg ctacgcccgc gaggacgatt tcggcgagtg ggccgagcgc ctggaaatct
      721 tcggactcga tctccgccac acgccgagcg tcgaggcgtt ctgccacgag atgctgacga
      781 cacgcgatcg tctcgacttc atcatcaaca acgcctgcca gaccgtccgc cgtccgccgg
      841 cattctacga gcacatgatg gagtccgaga ccgccgcgct ccacactctt tccgacgccg
      901 agcgtcacct gctcggcgtg ttcgaaggct tgcgcggcta ccggatgctc cccgaggcgt
      961 cggcgtcgat caagacgatg aagctcgaag aggtgaccgg gctgacgcat tcagcgcaac
     1021 tctcacaagt accgctgctg ccgggcgatc atgagcgcga tctctttccc gaaggccgtc
     1081 tcgatcagga cctgcagcag gtcgatttgc gcggtcgcaa ctcctggcgg ttgctgatgg
     1141 ccgaggtgtc gtcggtggag ttgctggaag tgcagctcgt caatgccgtc gcgcccttca
     1201 tcatcaacgc ccgcctcaag ccgctcatgc tgcggaccgc gaaccgcgac aagcacatcg
     1261 tcaacgtctc cgcggtcgag gggcagttct accgcaacca gaaaacgacg cgccatccgc
     1321 acacgaacat ggccaaagcc gcgctcaaca tgatgacccg caccgccgcc gccgactact
     1381 acaccgacgg catccacatg aacagcgtcg ataccggttg ggtgacggat gaagatccgg
     1441 ccgcgctggc cgagcgcaag accgcggtcc accgctttca tccgccattg gacatcgtcg
     1501 acggcgccgc ccgcatcctc gatccgatca tcaccggcat caacaccggc gagcacgtgt
     1561 ggggacagtt cctcaaagac tacaagccga cggattggta ggcgaacagt tcaaccacag
     1621 agacacagag gtcacagaga tagtgtcttc aactctgtgt cccctgtgtc tctgtggttg
     1681 atgcaaaaca cggttatgga aaagatcctc ggtcgcctcc gatcgtgcag ccgccccatg
     1741 cgccggtcga cggctcctcg gcgatcaccg ccatcttcgc tccgtcgcga tgccgtacgg
     1801 tgatcgtggt cgcgaatccc tgcttcgaag agaaatcgag ctggtcgaga cgattggtcg
     1861 atgtgaaggg gttctcgtgc gggaccacga cagcgactgt gaaacggagc gtgcccgact
     1921 cgagcgatgc gagctcgccc tcgatcggag cgacgttcac gcgtacctcg atctcgctgg
     1981 tcctcactcc cttcgctagg gatccgagtt tgatcgagca tgtcacgggc agctcgccgc
     2041 gatcgacgtc ttcaccgcgg gtggtggatg tcgcaagttc cgtcgccagg agctccgcca
     2101 tgacgggcat cgctgccaga gtcgatcgag tgccaaccca tttctgagca ttgatcgtca
     2161 gtccggcgcg caccgcccgg atctcgacat gccgcaactt gtcatccgag gggcggtcca
     2221 cctggtatgt gatgaccaca cgttgatcga gggcatcgag ccggacgccg atctccgacg
     2281 gatcggtgag aacgcttcca ccggtcgcat ccgccagcag tttcagtggt gcgagaggat
     2341 ccttgttgat gatcgtgtga ccgacattct cgcggacgtt gaaggaatgg ggtacgagtg
     2401 gctcatgcgt cgcggcagcg aagtgagtga cccgggtttc gatcggcgat gtggcgtagg
     2461 agagaaccgt ccacccttgc gcggccagag cctgagcgat ctcgttctgg tggatcgagg
     2521 cgttcagttc gcgcaactcc gtgggcaagt ccgggcgtat gtccccacga tcgatcgaat
     2581 aaaagccggc cggggcgaga tcgaacccgt cggtgacaaa caccagcgta cgcatcgagt
     2641 cgccggatgc atgccgagcg tatcgactca gccagccaaa catcgcgtcc tgctgtgccc
     2701 gaagaatcgt cgactcctga cgcgccgcct tccgcatcag tgaccggcgc gtcagtcgat
     2761 cgtctttgtc gctcgtgttt cgatcgccgg tcatgaactc ctgccgtatc cggatcagtt
     2821 tctctctccc tcgagcctcg tttccgagcg tctcgaagag cgagcgcagt gcggccgcgt
     2881 cgcgcgtcgg accgagcaac gagtgtgtcg aaggatcgtc cgtgatcacc tcgacgtcac
     2941 ccattgcgac gagcctgtcg acttgcggaa tcagcgactt gatggcgaga cgaagtcctt
     3001 cggtcgccga cagggactgc tgcagataga tgacaacctg ccacggcagt tgcggtttcg
     3061 atggcgtcgt gccgatcgat tccttcgtcg ccgtcgtcac tgccgaagcg ccgtggctgt
     3121 acgtcacgcc gatgatccgc tggcgcttgc cgtcctcgat caattcgaag tcgcccggct
     3181 tcaggtcact tggcagattt ccattcgcgt cgcggacagt tgccgacact tcgatggccg
     3241 tcacgttgat ccgggttccg tagttctgac cggaactgga tatcgggaag acgatgagcc
     3301 cggcgcaaac aattgaggct acatgacgga ggagcatgtc gcgaaacaga tgacagcatg
     3361 acgatgacat tgtcaaaagt ggaggccagt gatgaaatta ctcacagaga tagtgtcttc
     3421 aactccgtgt cctctgtgtc tctgtggtta
//
LOCUS       DATLVW010000559         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_520791, whole genome shotgun sequence.
ACCESSION   DATLVW010000559 DATLVW010000000
VERSION     DATLVW010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520791"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1430
                     /locus_tag="VNN25_16630"
     CDS             420..1430
                     /locus_tag="VNN25_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXH93211.1"
                     /translation="MKRALIALLSLAVAVSAFAAWSVVRHIPIAGTGGWDYTAIDSVA
                     RREYVSHGNNVDVVDVDSGKVVGVIPDTPGVHGIAIDHDGGRGFISDGRVSMMTIFNL
                     KTLNRITDVKTTGDNPDAIMFDPASQHVFTFNGRGKNATVFNTHGKVIATIDLGGKPE
                     FGVSDAKGHVFVNIEDTSELAMIDAKKNVVAKRWKLTPCESPSGLAIDRKHHRLFTVC
                     ENEIMAVVDATNGKVVTTVPTGKGTDAAAFDDARQLAFASNGQSGTLTVIHESAPDKY
                     EVVDNVATQRSARTMALDTKTHHIFLPAAQFGEAPPKTEKNPRPRPPMVPGSFELLEV
                     AP"
     gene            1521..>3193
                     /locus_tag="VNN25_16635"
     CDS             1521..>3193
                     /locus_tag="VNN25_16635"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXH93212.1"
                     /translation="MKRTAALLLTFLAAAASAQQTLIRTQKPPLHGSHWMAVTGKPLA
                     ATAGSMIFQKGGNAVDAAAAMIAATCTMWDTLSWGGETQVLIYDPHAKKVIGINALGV
                     APTGATPEFYKSKGFAHPPDEGVLAAVTPGTPGGLMTMVAEYGRLSLKDVLAPAIEMA
                     DGYALEAQSANTIDRNKDKLKAWPYSKKVFLIHENDPKHPGPVAGEIFRQPDLAATLR
                     KLVETEQLALKKGKSRKDAIYAAYDRFYKGDIAKEIVRGTREQGGLFTEEDLAGWKVH
                     IEEPVMTNYRGIDVYKLNVWTQGPAMLQALNILENFDLKSMGFNSPHYINAVYQAMSL
                     AWADRDFYYGDTYAPPEEPVLGLLSKDYAKDRAKLINPEHNDPKIKPGDPYPYQRATN
                     PFPDLLRNWDAPKAPVAKPAGTPESGSLRRNEFLHATPSLSNDYLFAFYAGTTSVEAA
                     DAEGWAVSVTPSGGWLPAVIAGNTGVGLSERMQSFVLDPAENPFNVVAPGKRPRVTLT
                     PTLALKDGKPFLCFSVQGGDTQDQNLLQFFLNVVEFGMSVQEACEAANLT"
BASE COUNT          692 a         1031 c          912 g          558 t
ORIGIN      
        1 cagagaaagt tctttccgac ctagagaaag ttctttccca cggagagaat attctttccc
       61 gcgcagagaa aattctttcc cacggagaga aagtttcagt ccgcgaagag aaggtttcac
      121 tccgcgaaga gaatgtttca ctccgcgaag agaaggtttc actccgcgga gagaaagttt
      181 tactccgcga agagaaggtt tcactccgcg gagagaaagt ttcactccgc gaagagaaag
      241 tttcactccg cgaagagaag gtttcactcc acggagagaa ggtttcactc cgcggagaga
      301 aagtttcact ccatggagag aaagtttcag tccgcataga aaaggtttca ctccagcttg
      361 gtctacctga acagcaattt cattgcgctc ggcccgcgtc ggcgataccg ttcgcgccca
      421 tgaaacgtgc actcatcgct ctcctctcgc tcgccgttgc cgtcagcgcc tttgccgcgt
      481 ggagtgtcgt ccggcacatt ccgatcgcgg gaaccggcgg atgggactac accgccatcg
      541 actccgttgc gcgccgagag tacgtctcgc acggaaacaa cgtcgacgtc gtcgacgtcg
      601 actccggcaa ggtcgtcggc gtcattcccg acactcccgg cgtgcacggc atcgccatcg
      661 atcatgacgg cggacgtggc ttcatctccg acggccgggt cagcatgatg acgatcttca
      721 atctgaagac gctgaatcgg atcaccgatg tgaagacgac cggcgacaac ccggacgcga
      781 tcatgttcga ccccgcctcg cagcacgtct tcacgttcaa cggccgcggc aagaacgcca
      841 cggtcttcaa cacccatggc aaagtcatcg ccaccatcga cctcggcggg aagccggagt
      901 tcggcgtcag cgacgcgaag ggccacgtct tcgtcaacat cgaagacacg agtgagctgg
      961 ccatgatcga cgcgaagaag aacgtcgtcg ccaagcgctg gaagctgacg ccgtgcgaat
     1021 ccccgagcgg tttggcgatc gaccgcaagc atcaccgtct cttcaccgtt tgcgagaacg
     1081 agatcatggc cgtcgtcgac gcaacgaacg gcaaggtcgt gaccacagtg ccgacaggga
     1141 aggggacgga tgcagcagct ttcgacgatg ccagacagct cgcgttcgcc tcgaacggcc
     1201 agagcggaac gctgaccgtc atccacgagt cagcccccga caagtacgaa gtcgtcgaca
     1261 acgtggccac gcagcgcagc gcccggacga tggcgctcga cacgaagacg catcacatct
     1321 tcctccccgc cgcgcagttc ggcgaagctc ctccgaagac cgaaaagaat ccgcgcccgc
     1381 ggccgccaat ggtgcctgga tcgttcgagc tcctcgaagt ggcgccgtaa ggcttatccg
     1441 cagatggcgc agatgtgcgc agatgttttt tttcatctgc gtttcatctg cggcatctgc
     1501 ggatcaataa tacgcggcgc atgaaaagaa ccgccgctct gcttctcacc tttctcgccg
     1561 ccgccgcatc cgcacagcaa acgctcatcc gcacccagaa gcctccgctg cacggatcgc
     1621 actggatggc cgtcaccggc aaaccgctcg cggccactgc cggctcgatg atctttcaga
     1681 aaggcggcaa cgcagtcgat gccgctgcgg cgatgatcgc tgcgacctgc acgatgtggg
     1741 acacgctctc gtggggcggc gagacgcagg tgctcatcta cgatccgcac gccaagaaag
     1801 tcatcggcat caacgccctc ggcgtcgctc ccaccggcgc gacccccgag ttctacaaaa
     1861 gcaaaggatt cgcgcatccg cccgacgaag gcgtcctcgc cgcggtcact cccggcacgc
     1921 ccggtggcct catgacgatg gtggccgagt acggccggct gtcgctgaag gatgttctcg
     1981 ctccggccat cgaaatggcg gacggctacg cgctcgaagc gcagtcggcg aacacgatcg
     2041 atcgcaacaa agacaagctg aaggcctggc cgtactccaa gaaagtcttt ctcatccacg
     2101 agaacgatcc gaagcatccc ggtccggtgg caggggagat cttccgccag cccgatcttg
     2161 ccgccacttt gcgcaagctc gtcgagaccg aacagctcgc actgaagaag gggaagtcac
     2221 gcaaggatgc gatctacgca gcctacgacc gcttctacaa aggcgacatc gccaaggaga
     2281 tcgtgcgtgg cacgcgcgag cagggcggtc tcttcaccga ggaggatctg gccggttgga
     2341 aggtccacat cgaagagccg gtgatgacga actatcgcgg catcgacgtc tacaaactga
     2401 acgtctggac gcagggtccg gccatgcttc aggcgctcaa catcctcgag aacttcgacc
     2461 tcaagtcgat gggcttcaac tcgccgcact acatcaacgc cgtctatcag gcgatgagcc
     2521 tggcctgggc tgaccgcgac ttctactacg gcgacacgta tgcgcctccc gaggaaccgg
     2581 tgctcggact gctctcgaag gactacgcga aagaccgcgc gaagctgatc aaccccgagc
     2641 acaacgatcc gaagatcaag ccgggcgatc cgtatccgta tcaacgcgcc acgaatccgt
     2701 tccccgatct gctgcgcaac tgggatgcac cgaaagcacc tgtcgcgaag ccggcgggga
     2761 caccggagtc aggctcgctg cgccgcaacg agtttctgca cgccacgccg tctctctcga
     2821 acgattacct cttcgccttt tacgccggca ccacctcggt cgaagcagca gacgcggaag
     2881 gatgggcagt atcggttaca ccgagcggcg gctggctccc tgctgtcatc gccggcaaca
     2941 ccggcgtcgg tctcagcgag cggatgcaga gcttcgtgct cgatcccgcc gagaacccgt
     3001 tcaacgtcgt cgcccccggc aagcgcccgc gcgtgacgct taccccgacg ctcgcgctca
     3061 aggacggaaa gccgtttctc tgcttttccg ttcagggtgg cgacacacag gaccagaatc
     3121 tcctccagtt cttcctcaac gtcgtcgagt ttggcatgag tgttcaggag gcgtgcgagg
     3181 cggcgaacct tac
//
LOCUS       DATLVW010000560         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_434948, whole genome shotgun sequence.
ACCESSION   DATLVW010000560 DATLVW010000000
VERSION     DATLVW010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434948"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VNN25_16640"
     CDS             complement(<1..200)
                     /locus_tag="VNN25_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93213.1"
                     /translation="MRSSSTSVVLAVVLSAAFASIARAADPPPLPAPAPTTVHRAEGP
                     ITVDGDLGDAGWKNAATIDQFY"
     gene            complement(273..857)
                     /locus_tag="VNN25_16645"
     CDS             complement(273..857)
                     /locus_tag="VNN25_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HXH93214.1"
                     /translation="MNPTFFATPTAFRKWLEKHHAEESELLVGFYKRDSGKPSITWPE
                     SVDQALCFGWIDGVRRRIDDVSYSIRFTPRKAISNWSAINIARVAELTTLGLMAPAGL
                     RAFEKRREDKSAIYSYEDKVRTLPPADEKQFRANRKAWAFFTGQAPSYQRVAIYWVTS
                     AKREETRVRRMATLIEDSANARRLAVVTLTPKKS"
     gene            1033..1377
                     /locus_tag="VNN25_16650"
     CDS             1033..1377
                     /locus_tag="VNN25_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXH93215.1"
                     /translation="MQYLLLIYDDEKRWSTMTEADMQKGMAAYNAFGEEFAGKIKGGN
                     ALQPTHTATTVRTRDGKRLTTDGPYAETKEALGGYYLVDANDLDEAIAIAGKIPGALT
                     GAIEVRPIMTFD"
     gene            1397..2620
                     /locus_tag="VNN25_16655"
     CDS             1397..2620
                     /locus_tag="VNN25_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH93216.1"
                     /translation="MTAIEDVFRSEWGRLLAALIHLLGDFDVAEEALQEAFAAAVTEW
                     AGEVPRNPRAWLYGTARHKAIDRLRRRARFAEKQAEIAYLAPSATSDEMTDADESGVP
                     DERLRLIFTCCHPALAEDAQVALTLRTLCGLSTEEIARAFLVPTATMAQRLVRAKAKI
                     RGAGIPYRVPPASELPERLSVVMAVVYLVFNEGYAAASGQSLVRHDLCAEAIRLARVL
                     RALLPDAQLDALLALMLLHDARRATRVDDNGEMVLLADQDRSRWDRTEIDEGRTLLQT
                     SLRTSRPGTYALEAAIAAVHADADIAADTDWKQIAAIYERLYFLHPTPIVALNHAVAV
                     SLSDGPAAALPLVDALSESLNDYHLWHATRADLLRRLGRDEDAVASYRRAHELAGNEA
                     ERRFLARRIAELTDS"
     gene            2781..>3255
                     /locus_tag="VNN25_16660"
     CDS             2781..>3255
                     /locus_tag="VNN25_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93217.1"
                     /translation="MKIKMSQRATLLVLVCATLLYSLPALAQFTQQGPKLVSAPALSS
                     SQGNSVAISADGNTAVIGGPSDNEYIGAVWIWTRSAGVWSQQSGKLIGSGAVCCANQG
                     NSVAISADGNTVLVGGSGDDSNNGNPVGAAWVWTRTGGVWSQQGAKLVGSGAEGFA"
BASE COUNT          626 a         1063 c          995 g          571 t
ORIGIN      
        1 tcgtagaact gatcgatcgt cgctgcgttc ttccagccgg catcgccgag gtcgccgtcg
       61 acggtgatcg gcccttctgc gcggtgaact gtcgtcggcg cgggagccgg gagcggcggc
      121 ggatcagcgg cccgcgcgat gctcgcaaaa gctgcagata agacaactgc caggacgacg
      181 gatgtcgacg atgagcgcat gcaaatgact cccccaatga ccgaatacgt aggtttgacg
      241 aagaagggtg cagggagtta gtgacaacac ggtcacgact tcttcggcgt cagggtgacg
      301 acggcgagcc ggcgggcgtt cgccgagtcc tcgatcagcg tggccatgcg gcgcacgcgc
      361 gtctcttccc gcttcgcact cgtgacccag tagatcgcca cgcgctgata cgacggagcc
      421 tgccccgtga agaacgccca cgctttcctg ttcgcccgga actgtttctc atcagcggga
      481 gggagcgtcc gcactttgtc ttcgtacgaa tagatcgcgg atttgtcttc gcgcctcttc
      541 tcgaacgcgc gcagtccggc cggcgccatc agtccgagcg tcgtcagctc cgccacgcgg
      601 gcgatattga tggcgctcca gttgctgatc gcctttcgcg gcgtgaagcg gatgctgtag
      661 ctgacgtcgt cgattctccg ccgcacgcca tcgatccacc caaagcaaag cgcctgatcg
      721 accgactcgg gccaggtgat cgacggcttc cccgaatccc tcttgtagaa ccccacaagc
      781 agttccgact cctcggcgtg atgcttctcg agccacttgc gaaaggccgt cggtgtcgcg
      841 aagaaggtgg ggttcatcac aatcagaata tcgcttaacg taggtcggac tctccagtcc
      901 gactgcgcgt gatgatgtcg gactggagag tccgacctac gttaagaaaa tgttgagcgc
      961 gcaaaaaaac tttcatcccc tgtcgatttc gcccttcccc gttcgactat cactcaaaag
     1021 gaggaaacac ctatgcaata cctgctgctg atttacgacg acgagaagcg ctggagcacc
     1081 atgaccgagg cggacatgca gaaagggatg gccgcgtaca acgcgttcgg cgaggagttc
     1141 gccggcaaga tcaaaggcgg caacgcgctg cagccgacgc acaccgcgac gaccgtccgc
     1201 acgcgcgacg gcaaacgcct caccaccgac ggaccctacg ccgaaacgaa ggaagccctc
     1261 ggcggctact acctcgtcga cgccaacgat ctcgacgagg ccatcgccat cgccggaaag
     1321 attccgggcg ctctcacggg cgcgatcgaa gtccgcccga tcatgacgtt cgactgatcg
     1381 ctatcatgca gcggtcttga ccgcgatcga agatgtgttt cgcagcgaat ggggccggct
     1441 cctggccgcc ctcattcacc tcctcggcga tttcgacgtc gccgaggagg cactgcagga
     1501 agcgtttgca gcggcggtga cggagtgggc cggcgaagtc ccgcgcaatc cgcgcgcatg
     1561 gctgtacgga acggcgcggc acaaagcgat cgaccgcttg cgccgccgcg cgcgtttcgc
     1621 ggagaagcag gcggagatcg cgtacctcgc gccgtccgca acgagtgatg agatgaccga
     1681 tgccgacgaa tccggcgtcc ccgacgagcg cctccgcctg atcttcacct gctgccaccc
     1741 ggcgctggcc gaggacgcgc aggtggcgct aacgctgcga acgctgtgcg gcttgtcgac
     1801 cgaggagatc gcacgcgcgt tcctggtccc gaccgcgacg atggcgcagc gcctcgttcg
     1861 ggcgaaggca aagatccgcg gcgccgggat tccgtatcgc gtaccgcccg catccgagct
     1921 tcccgagcgc ctgtccgtcg tgatggcggt cgtctatctg gtcttcaacg aaggctacgc
     1981 cgccgcaagc ggccagtcgc tggtgcgtca cgatctctgc gccgaagcga tccggctagc
     2041 aagagtgctg cgcgctctgc tccccgacgc ccaactcgac gccctcctcg cgctgatgct
     2101 gctccacgac gcgcgacgtg caacgcgcgt cgatgataac ggcgagatgg tcctcctcgc
     2161 cgatcaggac cgctcaagat gggaccgcac cgagatcgac gaaggccgca cgctgctgca
     2221 aacatcgctg cgcacatcgc gcccaggcac gtacgcgctc gaggcggcaa tcgctgccgt
     2281 tcacgcggac gcggacatcg ccgcggatac cgactggaaa caaatcgctg caatctacga
     2341 gcgcttgtac tttctccacc ccaccccgat cgtcgccctc aaccacgcag tggcagtatc
     2401 actgtccgac ggtcccgccg ccgcgttgcc actcgtggac gcattgtcgg aatcgctcaa
     2461 cgactaccac ctctggcacg ccacgcgcgc cgatctcctc cgccgcctcg gccgcgatga
     2521 ggatgcagtc gcaagctacc ggcgcgcgca cgagctggcg gggaacgagg cggagcggcg
     2581 gtttctcgcg cggaggattg cggagctgac ggattcttag caagtcgcgc agagcgtttc
     2641 aaccgcttcg cgttcttcga ggcttcgcgt gatacctgtg ttcacgcaga gacgttcacg
     2701 cgaaggcgcg aagacgcgaa gaactgagag caatatcttt gaactaaatc acggcagaac
     2761 accgttaccg cctccactcc atgaaaatca aaatgtctca gcgcgcgacg ctgcttgtcc
     2821 tcgtctgcgc cactctcctc tattctcttc ccgctctcgc acagttcacc caacaggggc
     2881 cgaagctggt gagcgcacct gcgttgagct cttcgcaagg taactcggtc gccatctcgg
     2941 cggacggcaa cacggctgtt atcggcggac ccagcgacaa cgagtacatc ggagccgtct
     3001 ggatctggac gaggagcgcg ggagtttgga gccagcagtc cggcaagctg atcggctccg
     3061 gtgctgtctg ttgcgcgaac caggggaact ccgtagccat ctccgcagac ggcaatacgg
     3121 tgcttgttgg aggatctgga gacgattcca acaacggcaa tcctgtcggt gcggcctggg
     3181 tctggaccag aactggcggt gtctggagcc aacagggagc gaagcttgtc ggctcaggcg
     3241 ccgaaggttt cgccg
//
LOCUS       DATLVW010000561         3278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_435055, whole genome shotgun sequence.
ACCESSION   DATLVW010000561 DATLVW010000000
VERSION     DATLVW010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435055"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..410)
                     /locus_tag="VNN25_16665"
     CDS             complement(204..410)
                     /locus_tag="VNN25_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93218.1"
                     /translation="MSIDGRAAAPISTSAAPQITPAIAANGVDFFVTWVEGNEVKVSR
                     VDATGHASPPAIAGASRQVVIRFC"
     gene            602..2545
                     /locus_tag="VNN25_16670"
     CDS             602..2545
                     /locus_tag="VNN25_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93219.1"
                     /translation="MRHVLFALFVALTAATAQAGSADLALEANTAPAVRAGDIFSIAT
                     LLHNLGPDVARNVVVTFQVDGLPDPQLPCPGGRCMLGDIQPGADANVTQFEQVLPASD
                     LTVTVEITVTSDTPDPHPDNNSVTLTIVGTTGPRLSAELQGPPVIDPGLPYDAILWVF
                     NTGLSAAHHVVATVDLPEGSAVQSVPSNCTAGPLQVTCTLERLAAGVTIINASPITMK
                     LRAPPRYEGGLIPFELRVHADEPDFGVPFDKPASALLYRTFLVTTSADDGDGSLRAAI
                     SAANAADCYSTITEGSPICAIQFNIGETSERPWKTIRLHSPLPFIGAYSLRIDGATQA
                     AFSGVTNPDGPPIEISGGGTVDGDGLDAGTCGVTITHLAINGFLGNGISLRNFNGFSG
                     FACKSSIEDNYIGTDPTGSVAVPNYRGIGTIQSNSPFFFGSIISGNVLSGNIRSGLSV
                     LAGIWQIRENRIGVKAHSDEPLPNGASGIYLDRGLYRSEVSFNVIAFNREMGIAIDPG
                     TLYTNIAYNRIWGNGGLAIDDGLDGPSVAVMGDNGPINVPVLTSAVYDPAKNQTIIHG
                     NASGSIQVFASDAAGPGGTGDAQRWIASWSGGGDFAMTVPGDLRGQWVSATVTAYAAK
                     DNPYPDVFSYVRTTELSRAMQVH"
     gene            2756..>3278
                     /locus_tag="VNN25_16675"
     CDS             2756..>3278
                     /locus_tag="VNN25_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93220.1"
                     /translation="MASVRLRLLCALLLVSPPLLANVVIDTIEINQAIGVQKNGNLKF
                     VAGKDAVVRAFLAEAVTIDESKTSATIRRDGHLVATIPPIAQQAPTRVVDFLCPTRDA
                     CGNWAAGNYEFEVRVNGTTRSTRGTTYAFVERATMRILAVPVTANYGGTIVPVSDSRW
                     KTFADYIRLTYPLA"
BASE COUNT          609 a         1091 c          994 g          584 t
ORIGIN      
        1 cgatcgcgaa cgctattgga cggaccagat cccccctctc gcgacggacg gtcgcatgag
       61 ccgaatcgga aacactctct tttatcgcca agcgcggcct ccggggagac ggattgaagg
      121 agtggcgcgg ggaatgatat ctccaccgga cacaatcgga cacccctaca catgcgagcc
      181 aggtccggca cgagcggtct gcgctagcag aaacggatca ccacctggcg cgaagcgccg
      241 gcgattgcgg gtggcgacgc gtggccagtc gcgtcgacgc gcgaaacctt gacctcgttc
      301 ccttcgaccc acgtcacgaa gaagtcgacg ccgttcgccg cgatggccgg tgtgatctga
      361 ggcgcggcgc tggtgctgat cggcgcggcc gcgcggccat cgatggacat ccagattgcg
      421 ttcgattcgc gccgggccga ttccgccgct gggcacgact gccgcgatgt ttctggacgt
      481 tgacgtggcg acgggcggtt tacaggcgcc gcgcccacac ttcgtacgtc cgataggttt
      541 aaataggggc ttcagctcgg cctgacgaca cttggccgag acctgcgcag ataggacgat
      601 catgagacac gtgctcttcg cgctcttcgt tgctctcacc gccgcaaccg cgcaagccgg
      661 gtcggccgat ctcgcgctcg aggccaacac ggccccggct gtccgggccg gcgacatctt
      721 cagcattgct acgcttcttc acaaccttgg tcccgacgtg gcccgcaatg tggtcgtcac
      781 gtttcaagtg gacgggctcc cggacccaca acttccctgc ccgggtggcc gttgcatgct
      841 cggcgatatt cagcccgggg cagatgcgaa tgtcacgcag ttcgagcaag tgctgccagc
      901 cagcgacctg acggtgacgg ttgaaatcac ggtcaccagc gacacccccg atccgcaccc
      961 cgacaacaac agcgtcaccc tcacgatcgt gggcacgact gggcccaggc tcagtgccga
     1021 attgcagggt ccaccggtga tcgatcccgg gctgccgtac gacgcgatcc tctgggtctt
     1081 caacacaggc ctctctgcgg cgcatcacgt tgtcgccact gtcgaccttc cggagggttc
     1141 ggccgtgcag tcggtgccat ccaactgcac ggccggtccg ttgcaggtga cctgcacgct
     1201 cgagcggctc gccgccggcg tcaccatcat caacgcgtct ccaataacga tgaaattgag
     1261 ggcacctcct cggtacgaag gcggcctgat ccccttcgaa ctgcgggtgc atgcggacga
     1321 accggacttc ggcgttccgt tcgacaagcc ggcgagcgcg ctgctttacc gcacgttcct
     1381 cgtcaccacg agcgctgacg atggtgacgg ctccctgcgc gccgcaatct cggcggcgaa
     1441 cgccgccgac tgctactcga caatcacgga gggctcgccg atctgtgcga tccagttcaa
     1501 catcggcgag acctcggagc ggccatggaa gacgatccgc ctgcactcgc cgctcccgtt
     1561 catcggcgcc tactccctcc gcatcgacgg tgccacgcag gctgcatttt ccggcgtgac
     1621 caatcccgac gggccgccca tcgagatcag cggcggtggg acggtcgacg gcgacggcct
     1681 cgacgcaggg acttgcggcg tgaccatcac ccacctggcc atcaatggct tcctcggcaa
     1741 cggcatctct ctgcgtaact tcaatggctt ctctgggttt gcatgtaagt cttcgatcga
     1801 ggacaactac atcggaacgg acccgaccgg atcagtggca gtgccgaact accgcggaat
     1861 cggcacgatc cagtcgaaca gcccgttctt cttcggatcg atcatctccg gcaacgtcct
     1921 ttccggcaac atccgcagcg gcctctctgt cctggccggc atctggcaaa tcagggagaa
     1981 ccgcatcggt gtgaaggcgc actccgacga gccgctgccc aatggcgcaa gcggtatcta
     2041 tctcgatcgt ggcctctacc ggagtgaagt gagtttcaac gtcatcgcct tcaatcggga
     2101 gatgggcatc gccatcgatc ccggcacctt gtacaccaac atcgcctaca accggatctg
     2161 gggcaacggc ggcctggcca tcgacgacgg actcgacggg ccgtcggtcg cagtgatggg
     2221 cgacaacggt ccgatcaacg tccccgtctt gacgagcgcc gtttacgacc cagcgaagaa
     2281 tcaaacgatc attcacggga acgcctcggg atcgatccag gtcttcgcga gcgatgccgc
     2341 aggccccggg ggaacgggcg acgcccaacg atggatcgcc tcctggagcg gtggcggcga
     2401 tttcgccatg accgttccgg gcgatctgcg cgggcagtgg gtatcggcga ccgtgaccgc
     2461 ctatgcggcg aaggacaacc cgtacccgga tgtcttcagc tacgtccgga cgacggagct
     2521 gagtcgcgcg atgcaggtac actaactggc cggcggctat ctcacgcggc gagcagagcg
     2581 gttcgaaaat cgtccacaat gctcccgccc cagccgtgaa tcagcgacga agcgatgaat
     2641 ccgggacatc atcgcaaggg ccgcaattcg aaaggcccgg cgcgttagtg gccaggcctc
     2701 ggcgttcctt ctcaccacgc tacgtctccg attacgaccg tgctactgta aatccgtggc
     2761 ttcagtccgg ttgcgccttc tctgtgcgtt gctgctggtg tctccgccgc tccttgcgaa
     2821 cgtcgtgatc gacacgatcg agatcaacca ggccatcggc gttcagaaaa acggcaactt
     2881 gaaattcgtt gcagggaagg atgccgtggt ccgcgcattt ctcgcggagg cggtgaccat
     2941 cgacgagtcg aaaacgagtg ccacgatccg gcgcgatgga catctcgtgg cgaccattcc
     3001 accgatcgca cagcaggcac cgacgcgcgt cgtcgatttt ctttgcccga ctcgcgacgc
     3061 gtgcggaaac tgggcggccg gcaactacga gttcgaggtg cgggtcaacg gaacgaccag
     3121 gagcacgcgc ggaacgactt acgccttcgt cgagcgcgcc acgatgcgca ttctcgcggt
     3181 gccggtgacc gcgaactacg gcggcacgat cgtgccggtc agcgactcgc gctggaaaac
     3241 gttcgccgac tacatccgtt tgacctatcc cctcgccg
//
LOCUS       DATLVW010000562         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_520869, whole genome shotgun sequence.
ACCESSION   DATLVW010000562 DATLVW010000000
VERSION     DATLVW010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520869"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /gene="groL"
                     /locus_tag="VNN25_16680"
     CDS             <1..1555
                     /gene="groL"
                     /locus_tag="VNN25_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688391.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXH93221.1"
                     /translation="AVKVTLGPKGRNVVLEKKFGGPNITKDGVTVAKEIELKDPLENM
                     GAQMVREVASKTSDVAGDGTTTATILAQSIYREGVKAVASGANPMALKRGIEKAVEVA
                     VEEVKKLSKPVTGEMIAQVGTISANSDHTIGNIIAEAMKKVGKDGVITVEESKTMTTE
                     LQTVDGMQFDRGYLSPYFVSDAERMECVLEDPYILIHEKKISNMKDLLPLLEQIARSG
                     KPLLVIAEEVEGEALATLVVNKLRGTLNACAVKAPGFGDRRKAMLEDIGILTGGKAIM
                     EETGIKLEGVRLEDLGRAKRVTVDKDNTTIVDGNGTQKSIEGRIKQLRAQIEETTSDY
                     DREKLQERLAKLAGGVAVIKVGAATETEMKEKKARVEDALHATRAAVEEGIVPGGGVA
                     LLRASKALKGLKLGGDEQIGVDIVRRASEEPLRQIVSNSGNEGAIVVEKIRESSDPNF
                     GYNAATDQYEDLVKSGVIDPTKVTRTALQNASSIASLMLTTEAMIAEIPEKKQAPAPG
                     GHGPEGMDY"
     gene            complement(1723..>2006)
                     /locus_tag="VNN25_16685"
     CDS             complement(1723..>2006)
                     /locus_tag="VNN25_16685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXH93222.1"
                     /translation="GTRQPFETVADLAWRDGRGRITQARGRCLDISESGARIAYSDRI
                     ELPAVMQIRTEHDGVLRTGRVRHCTPHGSQYEIGIEFCDPAQLKAAANT"
BASE COUNT          451 a          570 c          659 g          326 t
ORIGIN      
        1 tgccgtaaaa gtgaccctgg gccccaaggg ccggaacgtg gtcctggaga aaaagtttgg
       61 cgggccgaac atcaccaagg acggcgtgac ggtggccaaa gagatcgagc taaaggaccc
      121 gctcgagaac atgggcgcgc agatggttcg tgaagtggca tcgaagacct ccgacgttgc
      181 cggcgacggc acgacgacgg ccacgattct ggcgcagtcc atttaccgcg agggcgtcaa
      241 ggccgtcgca tccggcgcca atccgatggc gctgaagcgt ggcatcgaga aggccgttga
      301 agtggcagtc gaagaagtga agaagctctc caagcccgta acgggcgaga tgatcgcgca
      361 ggtgggcacg atttccgcca acagcgatca caccatcggc aacattatcg ctgaggcaat
      421 gaagaaggtc ggcaaagacg gtgtcatcac ggttgaggaa tcaaagacca tgaccaccga
      481 gctgcaaacc gtggacggta tgcagttcga ccgcggctat ctgtcgccct atttcgtcag
      541 tgacgccgag cgcatggagt gcgtgctcga agatccgtac atcctgattc acgaaaagaa
      601 gatcagcaac atgaaggacc tgctcccgct gctcgagcag atcgcgcgca gcggcaagcc
      661 gctgctggtg atcgccgagg aagtggaagg cgaggcgctg gccacgctgg tggtcaacaa
      721 gctgcgcggc acgctgaacg cttgcgccgt aaaagcgccg ggcttcggcg accgccgcaa
      781 ggcgatgctc gaagatatcg gcatcctgac cggcggcaag gccatcatgg aagagaccgg
      841 tatcaagctc gagggcgttc gcctcgagga tctgggccgc gccaaacgcg tcaccgtgga
      901 taaggacaac accacgatcg tggacggcaa cggcactcag aaatcgatcg aaggccgcat
      961 caagcagctg cgcgctcaaa tcgaggaaac cacctcggat tacgatcgcg agaaactgca
     1021 agagcgcctg gcgaagctgg ccgggggcgt ggcagtgatc aaggtcggcg cggccaccga
     1081 gaccgagatg aaggagaaga aggctcgcgt ggaagatgct ctgcatgcca ctcgcgcggc
     1141 ggtcgaggaa ggcatcgttc cgggcggcgg cgtagctctg ctgcgcgcat ccaaggccct
     1201 gaagggtctg aaactgggtg gcgacgagca gattggcgtc gacatcgtgc gccgggcctc
     1261 cgaagagccg ctgcggcaga tcgtgtcgaa ctcgggtaac gagggcgcga ttgtcgtgga
     1321 gaagattcgc gagagcagcg acccgaactt cggatacaac gccgctaccg atcaatacga
     1381 agatctggtg aagtcgggcg ttatcgatcc gaccaaagtg acccgcacgg cgctgcagaa
     1441 cgcgtcgtcg atcgcttccc tgatgctgac cacggaagcg atgatcgccg aaattcccga
     1501 gaagaaacag gctccggctc ctggcgggca cggacccgag gggatggatt actaaggcgc
     1561 aacctgacgc ggcagagcgc ggtcccaatg agtcaagggg gatcgcgcta ctcgcgcgaa
     1621 agtgctttcg gtaaccgaac agaagtcaca agccgcgctt cccgcaaagg gggggcggtt
     1681 ttttttgctt ccagcgtacc ggccacgcgt atttttttgt cgctacgtat tcgccgcggc
     1741 tttcagttga gccgggtcgc aaaattcgat gccgatttcg tactgcgatc cgtggggcgt
     1801 gcaatgacgt acccggccgg ttctcaatac gccgtcgtgt tccgtacgaa tttgcatgac
     1861 ggcggggagt tcgatacgat ccgaatacgc gatgcgagcg ccggattctg agatatcgag
     1921 acaccggcca cgcgcctgcg taatccttcc gcgaccgtcg cgccaggcga gatcggcgac
     1981 cgtctcgaag ggctgccgcg tgccga
//
LOCUS       DATLVW010000563         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_394503, whole genome shotgun sequence.
ACCESSION   DATLVW010000563 DATLVW010000000
VERSION     DATLVW010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394503"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VNN25_16690"
     CDS             complement(<1..499)
                     /locus_tag="VNN25_16690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXH93223.1"
                     /translation="MDERAKIMRGQSEQQSSIPKADALYARQLEAINEVARIATLDLE
                     LRPLMQRITDALAQKFEWEFVALVTVEPRKGAFVCEAVTSTVPTEVHAGYSRPLGSGV
                     VGTVAATEKAILLDDVGTYPDFVETMPGTKSELCVPVLHGKELVAILNLESTHLRAFH
                     DQVSLL"
     gene            740..1783
                     /locus_tag="VNN25_16695"
     CDS             740..1783
                     /locus_tag="VNN25_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXH93224.1"
                     /translation="MPRTLIGIALVSFVLSGCAGKEAAPVKSGSSESRTLSSAASTAA
                     SSSTVATTTTSAPSDSQEPSLVSFSSGALIAQKPQEYNETWSAFRILDEKATTGWATP
                     KGVITPQTIVVALSEKTELNRLEFDTASVDGDHRGAKDVSVEVSDVSPTSGFQKIADV
                     SLQDKADKQSFPVPAAVSGRWVRLNVKNNWGSPDYVELMDFRGYGKQLTQTAFANASG
                     TYATNFGNFHLKQEGTSVTGCYEHSDGLLTGGIEGRIMKFTWTQRGGAKGPAIMVFDP
                     SGQQMFGLWWYDSDSNGAGSEWNGKKISSDVGSCPHWAGSGAEATMEKELEDAGRTRV
                     YGINFDTKFRRDP"
     gene            1773..>2027
                     /locus_tag="VNN25_16700"
     CDS             1773..>2027
                     /locus_tag="VNN25_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXH93225.1"
                     /translation="MIRDESKPTIDKMAAMLKAKPDWKLMIEGHTDSSGGDAQNLPLS
                     QRRADSVKAYLVNAGIAAGRLTTKGLGASQPLAGNETATGR"
BASE COUNT          409 a          617 c          636 g          365 t
ORIGIN      
        1 tgaggagcga aacctgatcg tggaacgcac gaaggtgcgt gctctcgaga ttgaggatgg
       61 ccacgagctc tttgccatgc agcacgggaa cgcacagctc cgacttggtt cccggcatcg
      121 tctcgacgaa gtcaggatag gttccgacat cgtcgagcag aattgccttc tcggtcgctg
      181 ctacggttcc gactacaccg ctgccgagcg gacggctgta gccggcgtga acctcggtgg
      241 gaaccgtgct ggtcaccgct tcgcaaacga aggcgccctt gcgcggctcg accgtcacca
      301 gcgctacgaa ctcccactcg aacttctgcg ccagagcgtc ggtgatgcgc tgcatgagcg
      361 ggcgaagctc gaggtcgagc gtagcgatgc gcgcgacctc gttgatggct tcgagttgac
      421 gcgcatagag cgcatccgct ttcggaatcg acgactgctg ctccgattgg cctcgcatga
      481 tcttcgcgcg ttcatccata gcacgagcgg aagcgtctga cgctgattcc gtgccgattc
      541 ggaagcgtga gccgcctgtg gtctatctct gccggaccgc tacggtggta atcgcacgcg
      601 tccgtctgct gcggcgggag cgaatccgag tacgcgagca cgatgacgtt cgcgctcttc
      661 cggctgggct cctttcgata gtcaacgacc atcgtacaat ccgggccgga acgttttcag
      721 agatcgacag gagtcatgaa tgcctcgtac tttgatcggg atcgccctcg tgtcgttcgt
      781 gttgtcgggc tgcgctggga aggaagctgc cccggtgaag tccggcagct cggagagtag
      841 gacgttgagt agcgctgcca gcactgccgc cagcagctcg acagtggcga cgacgacaac
      901 ctctgcgcct tccgatagcc aggagccgtc gctcgtctcc ttttcttcgg gtgcgctcat
      961 cgcgcagaaa ccgcaggagt acaacgaaac ctggtccgct ttccgtatcc tcgatgagaa
     1021 ggctacgacc ggctgggcta ccccgaaggg tgtgatcaca ccgcagacga tcgtcgtcgc
     1081 gctttccgaa aagacggagc tcaatcgact cgagttcgat accgccagcg tcgacggcga
     1141 ccaccgcggc gcgaaggatg tctccgtcga ggtctccgat gtcagtccta cgtccggctt
     1201 tcagaaaatc gcagacgtct cgctgcagga caaggccgac aagcagagtt ttccggtccc
     1261 tgctgccgtc tccggtcgtt gggtcaggct caacgtcaag aacaactggg gctctcccga
     1321 ctacgtcgag ctgatggact tccgaggcta cggcaagcag ctcacgcaaa ccgcatttgc
     1381 gaacgccagc ggcacgtacg ccacgaactt cggaaacttt cacctcaagc aggagggaac
     1441 atctgtgacc ggctgctacg agcactcgga tggactcctg accggaggca tcgagggccg
     1501 gatcatgaag ttcacctgga cgcagagagg cggcgcgaag ggacccgcca tcatggtctt
     1561 cgatccaagc ggccagcaga tgttcggact ctggtggtac gacagcgatt cgaacggcgc
     1621 aggcagcgag tggaacggca agaagatttc cagcgacgtc ggctcctgtc ctcactgggc
     1681 gggtagcgga gccgaggcga cgatggagaa ggagttggag gacgctggcc gcacacgcgt
     1741 ctacggcatc aacttcgaca ccaaattccg acgtgatccg tgatgagtcg aagccgacga
     1801 tcgacaagat ggcggccatg ctgaaggcga agcccgactg gaagctcatg atcgaaggcc
     1861 atacggattc gagcggcggg gacgcgcaga atcttcctct ctcgcaacgc cgcgcggact
     1921 cggtgaaggc gtatctggta aacgccggaa tcgccgccgg ccgcctcacg accaaaggcc
     1981 tcggcgcgtc gcaaccgctg gccggcaacg agaccgcaac cggacgg
//
LOCUS       DATLVW010000564         3005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_233904, whole genome shotgun sequence.
ACCESSION   DATLVW010000564 DATLVW010000000
VERSION     DATLVW010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233904"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VNN25_16705"
     CDS             complement(<1..193)
                     /locus_tag="VNN25_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93226.1"
                     /translation="MSNKTRTAAVVVVIVAFLAGILVGVAGDHFYLIRLFPRHTARFS
                     ADRMAERLAGELELTPQQKT"
     gene            complement(183..500)
                     /locus_tag="VNN25_16710"
     CDS             complement(183..500)
                     /locus_tag="VNN25_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93227.1"
                     /translation="MIDDTFLEQLRDDARQLQYEPDDVMTTRIAARVRARVSEEAAGG
                     VAQLLARWFRPVMVSLAALSLAAVVGVGWLELSPEPAPGLDAVSSSQTVDVSVGGDVF
                     SVE"
     gene            complement(493..1149)
                     /locus_tag="VNN25_16715"
     CDS             complement(493..1149)
                     /locus_tag="VNN25_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH93228.1"
                     /translation="MSATSMFGGASIGSLALDRSVFTAERAQTRSATNEDPIEARAIT
                     AVKAGDAGSYGYLVSKYTKRVVSIAWGIVRNAADAEDLAQEAFVKAYENIARFKSGEP
                     FGPWIYRIVTNLGLDLIKHRKRFRHEEITDAAPAERRDRADLPAITNELAARIDHAIQ
                     SLPEMQRIVARLYLVEQFGHTEIAAMTNLTEGTVRSHLSLARGKLKEKLADLHGGNND
                     "
     gene            complement(1157..1636)
                     /locus_tag="VNN25_16720"
     CDS             complement(1157..1636)
                     /locus_tag="VNN25_16720"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXH93229.1"
                     /translation="MKKRILIASAVAVLAILIIVPIALAQHGLGMGRGHGCMMAGGRS
                     GMMMFGHLGRAKAALNLSDQQAADIKKIFADLRTQNEPYRASMRSGMQSVAQALIANP
                     NDIAAAQALLDKQSEAEHAMKTNALNAAAKALNVLTPDQRAKAATFLQQRIARQAEK"
     gene            1852..>3005
                     /locus_tag="VNN25_16725"
     CDS             1852..>3005
                     /locus_tag="VNN25_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93230.1"
                     /translation="MFRPDNRGFIEVYGINQFASQAACDRARDVQFKRNMAVVEFFRP
                     QRRADWWKADTFGPCHCDMTVETASPNMLTESARWAQVQLANDVRQRVRERLLDAGVL
                     SDSELIRGLAAPMVTAPLIGGPKLVTMPQAVATTTASNSPDDLRITKAVDATAASASS
                     LDLPLAEIPLPGVASPVTPAPAPTTPAPSPATLVVAQQPTSAPQPTAGPVPAPVPAPA
                     PLGPPPAIESTVVVADEPELPAPEDVADAFISVESQRIQNVLKVSGSIGDESIKAQVL
                     DACTQRIQLLSNLRSIIVGSGAKSRLVAAANGAKKESERLALVSRLFGSDVGPHWAPK
                     DAEDVVFDPGVTDPERVLRDVTTSDQQKRHALYALLATSSPTEQQQLWLTS"
BASE COUNT          531 a          976 c          924 g          574 t
ORIGIN      
        1 gcgttttctg ctgcggcgtg agctcgagct cgcccgcgag ccgttcggcc atccggtcgg
       61 cggaaaagcg tgctgtgtgc cgcggaaaaa gacggatcag atagaagtga tcgccagcca
      121 cgccgacgag aatgccggcg aggaacgcga cgatcacgac cacgacggcc gcggtgcggg
      181 tcttattcga cactgaacac atctccaccg accgagacat cgacggtctg gctgctcgaa
      241 accgcgtcga ggccaggcgc cggttcaggg gagagctcca gccagccgac gccgacgacc
      301 gccgccagcg aaagcgccgc gagcgagacc atgaccggcc ggaaccagcg cgccagaagc
      361 tgcgccacgc ctcccgccgc ttcttcggac acacgcgcgc gaacgcgggc cgcgatgcgc
      421 gtggtcatta cgtcatccgg ttcgtactga agttgccggg cgtcgtcgcg caattgctcg
      481 aggaaggtgt cgtcaatcat tgttgccccc gtgaaggtct gccagtttct ctttcagctt
      541 gccccgtgcc agcgacaaat gcgaccgcac cgtgccctcg gtcagattgg tcatcgctgc
      601 gatctccgtg tggccgaact gctcgacgag atacaggcgg gccacgatac gctgcatctc
      661 cggaagcgac tggatagcgt gatcgatccg cgccgccagc tcgttcgtga tcgccggaag
      721 gtcggcacgg tcacgccgct ccgccggtgc cgcgtcggtg atctcttcat gccggaaccg
      781 tttgcggtgc ttgatcagat cgaggccgag gttcgtgacg atccgataga tccacggccc
      841 gaaaggctcg ccggacttga aacgggcgat gttctcgtac gccttgacga aagcctcctg
      901 cgccaggtct tccgcgtcgg ccgcattccg cacgattccc caggcgatcg agaccacacg
      961 cttcgtgtat ttcgacacca ggtagccgta cgaaccggca tcgcccgctt tgaccgcggt
     1021 aatcgcgcgc gcttcgatcg ggtcttcgtt tgttgcgctt ctcgtctgcg ccctctccgc
     1081 cgtgaaaacc gatcgatcaa gcgcgaggga gccaatagag gctcctccga acatcgacgt
     1141 ggcgctcatc atcttcttac ttctcggcct gtctggctat ccgctgctgc aggaacgttg
     1201 cagctttggc gcgctggtcc ggagtgagga cgttgagtgc tttcgcggcg gcgttgagag
     1261 cgttggtttt catggcgtgc tcggcttcgc tctgcttgtc gagtagcgcc tgcgcggcgg
     1321 cgatatcatt cggattcgcg atcagagcct gcgcgacaga ctgcatgccg ctgcgcatcg
     1381 aagcgcggta cggctcgttc tgcgtgcgca gatcggcgaa gatcttcttg atgtcggcag
     1441 cctgctgatc cgagaggttg agagctgctt tggcgcggcc gaggtgaccg aacatcatca
     1501 tgccactgcg gccgccggcc atcatgcagc catgtccgcg gcccatccca agaccgtgct
     1561 gcgccagcgc gatcggaacg atgatgagta tggccaggac cgcaacggcc gatgcgatga
     1621 gaatgcgctt cttcatgggt tctccttgtt ggcgaacggg ttacgacgat gccatacgaa
     1681 cgttgaacat ataatcccgg caagtcgcgc cgctccatta tgcgaactct ttcagcaatc
     1741 gttgtgcttg tgtgcgcgtg cgtcagctcc gcgctcgcgc aaccgcttgt ctgcccgccg
     1801 gtcgccaccg ctgccggccg gccatgcgag accttccatt atcacgtgct gatgtttcgg
     1861 cccgataacc gcggattcat cgaggtgtac ggtatcaatc aattcgcatc gcaggccgct
     1921 tgcgaccgcg cgcgcgacgt tcagttcaag cgcaacatgg cggtggtcga gttcttccgc
     1981 ccccagcggc gggctgactg gtggaaggct gatacgttcg gcccgtgcca ctgcgacatg
     2041 acggttgaaa ctgccagtcc gaacatgttg acggagtccg ctcgctgggc acaagtgcag
     2101 cttgcgaacg atgtccggca gcgggtccga gagcgattac tcgatgccgg tgtgctgtca
     2161 gattccgagc tgattcgcgg acttgcggcg ccgatggtca ccgctccgct gatcggcggt
     2221 ccgaaactcg tcacgatgcc gcaggcagtt gcgacaacga ctgcatccaa cagcccggat
     2281 gatcttcgga tcacgaaggc cgtcgatgcg acagcggctt cggcctcctc gctcgatctg
     2341 ccgctggcag agatcccgct tccgggcgtc gcatcgccgg tcactccagc gccggcacca
     2401 acgacacctg cgccatctcc ggctacgctc gtcgttgcgc agcaaccaac gtccgcgccg
     2461 cagccaacgg cggggcctgt ccctgcaccc gttcccgccc cggcacccct cggtcctccc
     2521 cctgcgattg agtctacggt tgttgttgct gatgaaccgg agttgccagc gcccgaagat
     2581 gtcgcggacg cattcatcag cgttgaatcc cagcgcatcc agaacgtgct gaaagtcagc
     2641 ggatcgatcg gcgacgaatc aatcaaggcg caggtcctcg acgcctgcac acagcgcatc
     2701 cagctccttt cgaaccttcg ttcgatcatc gtcggttcgg gagcgaagag ccgtctggtc
     2761 gccgccgcca acggcgcgaa aaaagagagc gagcggctgg ccctcgtgtc gagactcttc
     2821 ggcagcgatg tcgggccgca ctgggcgccg aaagatgcgg aagacgtcgt cttcgacccg
     2881 ggagtgaccg atcccgagcg cgtgctgcgt gacgtgacca catcggatca acagaagcgg
     2941 cacgcactct acgcgctgct ggcgacgtca tcaccgaccg agcagcagca gttatggctc
     3001 acttc
//
LOCUS       DATLVW010000565         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_358679, whole genome shotgun sequence.
ACCESSION   DATLVW010000565 DATLVW010000000
VERSION     DATLVW010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358679"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VNN25_16730"
     CDS             <1..1415
                     /locus_tag="VNN25_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HXH93231.1"
                     /translation="PVPEILPDEVRAVRSDAIICTGRSDYPNQVNNVLGFPFIFRGAL
                     DVRATEINEEMKMAATRALALLAKEDVPDSVASLYGLRDVVFGPDYLIPFPFDPRVLL
                     WVAPAVAWAAVASGVAKEMIDLEQYREQLEARLGRARGIMRGIINRAVRDPKRIVFPE
                     GEEPKIIRAARMIVDEGMGHPILLGQRANIERIAAEGHISLRDIVIDDPGKSEKRDKY
                     ADYLWKRRQRKGLSHGEAHQLLFNGNYFGSVMVACGDADALLSGVGMHYPETIRPALQ
                     VIGPHPKAEIVSGLYMLVFEKHVIFCGDTTVNIDPTAEQLAQIAYSAGRIVQTFGITP
                     KIAMLSFSNFGSVRHPEAEKVAKAVAILRKRDAALVVDGEMQADTAMDENILRSAYPF
                     SMLKERANVLIFPNLSAGNIAYKLLHHLGGATKIGPILLGMNLPVHVLEQGADVQDIV
                     NMAAVAVIDAQQRINEGKAT"
     gene            1415..>2456
                     /locus_tag="VNN25_16735"
     CDS             1415..>2456
                     /locus_tag="VNN25_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXH93232.1"
                     /translation="MATEMKPQRKQVARESSIGLSRQGLEPRGEVHWNLIAPELFQAA
                     ARRNEGEFADMGPFVAVTTPHTGRSPNDKFVVKEPSSEKDVDWGKVNKPLADENFQLL
                     LEDVRQYLNESSELFVEDLYCGADPSYRLSVRYVSPNAWHMAFVRNMFIRPELSDLPT
                     FDPNFTVFHAPEFEADPSRHGTRSGTFIVLNIAERTILIGGTRYAGELKKAMFTVMNY
                     LLPKQGVLSMHCSANVGRSGDTALFFGLSGTGKTTLSADPDRRLIGDDEHGWSKDGIF
                     NFEGGCYAKVINLTPESEPEIYRTTQMFGTVLENVVLDPANRQVRFADQSITENTRAS
                     YPLHYIRNFVEGG"
BASE COUNT          517 a          768 c          727 g          444 t
ORIGIN      
        1 acccggtgcc ggagatcctc cccgacgaag ttcgcgccgt gcgttccgac gccatcatct
       61 gcacggggcg cagcgactat ccgaaccagg tcaacaacgt cctcggcttt ccgttcatct
      121 ttcgcggcgc gctcgacgtg cgcgccaccg agatcaacga agaaatgaag atggcggcga
      181 cgcgcgcgct tgcgctgctc gccaaggaag acgtgcccga cagcgtcgcg tcgctctacg
      241 gcctgcgcga cgtcgtgttc ggtcccgact atctgattcc cttccccttc gatccgcggg
      301 ttctgctctg ggtcgcgccg gctgtcgcct gggccgccgt tgccagcggc gtcgcaaagg
      361 agatgataga cctcgagcaa taccgcgagc agctcgaggc gcgcctcggc cgcgcgcgcg
      421 gcatcatgcg agggatcatc aaccgcgcgg tgcgcgatcc gaaacgaatc gtgttccccg
      481 agggcgaaga gccgaagatc attcgcgccg cgcgcatgat cgtcgatgag ggcatgggcc
      541 acccaattct cctgggccag cgcgccaaca tcgagcgcat tgccgccgaa ggccacatct
      601 cgctgcgcga catcgtaatc gatgacccgg ggaaatccga gaagcgcgac aagtacgcgg
      661 actatctgtg gaaacgccgg cagcgaaagg gattaagtca cggcgaagca catcagctcc
      721 tgttcaacgg gaattacttc ggatccgtaa tggtcgcctg tggcgatgcg gacgcgctcc
      781 tctcgggagt aggcatgcac taccccgaga caatccgtcc cgcgctgcag gtcatcgggc
      841 cacatccaaa ggccgagatc gtcagcggcc tctacatgct cgtcttcgag aagcacgtta
      901 tcttctgcgg agacaccacc gtcaacatcg atccgacagc cgagcagctc gcacagatag
      961 cgtactccgc gggcaggatc gttcagacct tcggcatcac gcccaagatc gcgatgctgt
     1021 cgttctccaa cttcgggtca gtgcgtcatc cggaagcgga gaaggttgcg aaggccgtcg
     1081 cgatcctgag aaagcgcgac gccgcgctgg tcgtcgacgg cgagatgcag gcggatacgg
     1141 cgatggacga gaacatcctg cgttcggcgt atccattcag catgctgaag gagcgggcga
     1201 acgtgctcat tttccccaac ctgagcgcgg ggaacatcgc ctacaagctc ctgcaccacc
     1261 tcggtggagc gacgaagatc ggtccgatcc tgctgggtat gaatcttccc gtgcatgtac
     1321 tcgaacaagg cgctgacgtt caggacatcg tgaacatggc cgccgtcgcc gtcatcgatg
     1381 cgcagcagag gatcaacgaa gggaaggcca cgtaatggca accgaaatga aaccgcagcg
     1441 caagcaggtg gcgcgcgaga gcagcattgg actgagcaga cagggcctcg agccgcgcgg
     1501 cgaggttcat tggaatttga ttgcgcccga gctttttcag gcggcggcac gtcgtaacga
     1561 gggcgaattt gcggacatgg gcccattcgt cgccgtaacc acaccgcata cgggacggtc
     1621 tccgaacgac aagttcgtcg taaaggagcc gagctccgag aaggacgtcg actggggcaa
     1681 ggtcaacaag ccgcttgcag acgagaactt ccagctgcta ctcgaggacg tccgccaata
     1741 cctgaacgaa tcatccgagc tattcgtcga agacctctat tgtggcgccg atccatcata
     1801 caggttgtca gtgcgctacg tctcacccaa cgcttggcac atggcgttcg tccgaaacat
     1861 gttcatccgg ccggagctga gcgacctccc gacgttcgat cccaatttca cggtttttca
     1921 cgcacccgag ttcgaggccg atccctcgcg ccacggcacg cgatccggga ccttcatcgt
     1981 tctcaacatc gccgagcgta cgattctcat cggaggcacg cgctacgccg gagaattgaa
     2041 gaaggcgatg ttcacggtca tgaattacct gcttccaaag cagggcgtgc tctccatgca
     2101 ttgctctgca aacgttggaa ggtccggcga cacagccttg ttcttcgggc tttccggaac
     2161 cggaaaaacg acactttcag ctgaccccga tcgccggctg attggcgacg atgagcacgg
     2221 ttggtcgaag gacgggatct tcaatttcga gggtggctgc tacgcgaagg tcatcaacct
     2281 tactcccgag agcgagccgg agatctatcg cacgacccag atgttcggca ccgtcctcga
     2341 gaatgtggtg ctcgatccgg ccaacagaca ggtccggttt gccgaccagt caatcacgga
     2401 gaacacgcgc gcttcctatc ccctccacta catccgcaac ttcgtcgagg gcggac
//
LOCUS       DATLVW010000566        10798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_233934, whole genome shotgun sequence.
ACCESSION   DATLVW010000566 DATLVW010000000
VERSION     DATLVW010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233934"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VNN25_16740"
     CDS             <1..289
                     /locus_tag="VNN25_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93233.1"
                     /translation="TNERQVTHNGAANFCPYFMNDGKRLIFASNVNNTGFNFDLWTIG
                     KDGQGLERITTAPGFDGFCVFSPDGQYLIWASGRAKPESHEMNLFIAKWVD"
     gene            complement(400..876)
                     /locus_tag="VNN25_16745"
     CDS             complement(400..876)
                     /locus_tag="VNN25_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93234.1"
                     /translation="MRRLAFVLLLVVPIACATRQPGTTKTKTTRGHGAIELTVTPNPV
                     LANNTGGSGYEFPFDVVVKETGGHPVTIDRVTANVYAGGGISVASESYDAPKIQSLGF
                     ATSIPAKGELHYHFNPKKEITDTSLFSSVYGDVRVEAIDDNGSRASSTATISVKKK"
     gene            complement(873..1433)
                     /locus_tag="VNN25_16750"
     CDS             complement(873..1433)
                     /locus_tag="VNN25_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXH93235.1"
                     /translation="MRKVLVVPIVLLFLACGHSRGPMANATIDSTSGSTAKGTVHFQD
                     AGENGVEVVVDLTGVPPGVHGFHVHEKGDCGNNAQNAGGHFNPNGMVHGAPDAVSHHA
                     GDFGNVTADDKGEVHTRFTTHSISINTGTNAVVGHAVVLHGNPDDLVSQPAGNAGPRI
                     GCGVVTLANAVASVNGPMMFPVRRTT"
     gene            complement(1514..2944)
                     /locus_tag="VNN25_16755"
     CDS             complement(1514..2944)
                     /locus_tag="VNN25_16755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH93236.1"
                     /translation="MPELFDVIVIGGGTAGLVIASGCARLGRKVALIEREKLGGDCLW
                     TGCVPTKALVASARLAHQLRHADAYGLEPQSVRVTPKSIMDSMRAQRHAIEHHDDPEK
                     FRTLGIDVIFGNARLISRKEVDVDGRRLIAKDIVIATGTRTAIPPVERLAENGFLDHV
                     SFLNQDSFPASLLILGGGYIGIEFAQMFRRFGSEVVVVEMLDEIINKEDHDVIARLRR
                     ILGDEGIDIRSGWGAKSVRLDGGKKVVRVENKLGETKELRVDEIFVASGRRANTENLG
                     LEDAGIKTNRGFVAVDKYLQTSVPRIWACGDVHGGMQFTHVAAFEAVKLVRNMLFPGR
                     SPVSYDNIPWGLYTDPEVGHIGMTETEATAALGNENVRTYSVEMAEVDRAVVDRTTGG
                     FVKLVCDKRGRILGAHALCANASTLIETLVLARQKKMTVAQLAGLVSPYPSLADALGK
                     AASMYYQDVAKGWLGKLGKKIAKWSQ"
     gene            complement(3076..3915)
                     /locus_tag="VNN25_16760"
     CDS             complement(3076..3915)
                     /locus_tag="VNN25_16760"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXH93237.1"
                     /translation="MAKQVVKVVAHRGMGQGFVQKDAPPENTLPAFSEGWKIAGACEL
                     DVHLTKDGQVIVIHDDTTNRTTNANWTVRDRFANELRGLDAGRWKAQKWAGTRLPLLE
                     DVLEAMPHAVDDACELFIELKDGPQVVPPAAEIVRQSGKAANVVLISFDIDTIIAAKH
                     ELPDNDCLLIVVFEADYNNGGWTVLYDEGPGFRTVTKTYVEDDLIALIKQYDLNGIDT
                     SFAVPPSLITRLHDEKLRSVVWTVDEPSIAIDMVRLGIHTITTDRPGPIRDALIAAGY
                     EVR"
     gene            complement(3908..4309)
                     /locus_tag="VNN25_16765"
     CDS             complement(3908..4309)
                     /locus_tag="VNN25_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXH93238.1"
                     /translation="MLREQSFTVRTTAREQILLITPDVQRALSALTLNDGIATIIVPH
                     TTCAISVNENADPDVPADLTKALRALVPKVRFAHGEGNSDAHFLSLLIGTSLSWPYRN
                     GQLILGTWQGIYFIELDGPRPARKVTVYVNG"
     gene            4490..6091
                     /locus_tag="VNN25_16770"
     CDS             4490..6091
                     /locus_tag="VNN25_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH93239.1"
                     /translation="MPFFCIHCRSEIDSKFKACPYCGEPITDFLRRYLEHPIDGKYQI
                     LARLGVGGMGEVYKVLHLHLNAIRVIKLMRASIASEPGADDRFIREARLATRINHPNV
                     AALYDFSTLEDGSRYMVWEYIEGTNLHELIETNGPLTPRYAAKLAIEALHGLEAIHRA
                     GIVHRDISPENIMICPDEEGTQHVKLIDLGIAKQGDATDASKTATGMFVGKWKYCSPE
                     HLGMLDAGERIDGRADLYSFGIVLYEMLTGVPPFQADTPHAYLMLHSSEKPRALAEVN
                     PSMPSSPALEALIFKALEKDRKKRFASAREFARAIEAIEPSLNDQVGASRPLARAVEV
                     TEEPTKVATPTQLSGGAQPKALAIDSKAPTELTDHGKPIANRELRKVSTTNVVPLPPR
                     PRARIAWFAAVAVLLALVIVAIVVRVKPSPEVAPVKETPAVTASNPVSVAPGRVALNA
                     FPWGEVTSVRNVATGTAVSLKEPVTPLTLELAPGTYEITMSNPGFATPITRKVEVKGG
                     ADQPVNIQFADPATATLPRFEDVAQ"
     gene            6088..7437
                     /locus_tag="VNN25_16775"
     CDS             6088..7437
                     /locus_tag="VNN25_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93240.1"
                     /translation="MKRAMLVLLCLAAWPALAEEKWLDAYNRGVSAVRANNFQPGAQA
                     LQKAIEEMPQENAAQRVRDQIFTYVPHFWLGIARLNLADPDGALREWKTSEEQGAIKS
                     TPYYSQLQELIGRANSEKQRRAQVAANPAKQEANAAISRALSAQMDAVTAGGDRSDTY
                     HAAQRKLGEAKALSAKGGLDVRVFKHASDVAEEARGLFATAADDAKKQRAARPPKTNP
                     VQERKPLEVVVPFDEGPPPKPVIQPPPPHTTAPVTQTSAPPPPKTDVKPKVAEPPPEE
                     SEALVAARIAVQQYRRRLLASNMPTTDAQRLDRQLTPQSDSNLIRRVVDEIAAKEREL
                     DKKAAAIPGKPAEIPASDPTAGELESAYRAYAAGDLASSDRILTNVLSSKPSAEAYLL
                     RGCSRYTQAMLSRDGNALLASAASDIQAALRINHSLRLDRGAWSPKLIAFFEKVKSQ"
     gene            complement(7529..9763)
                     /locus_tag="VNN25_16780"
     CDS             complement(7529..9763)
                     /locus_tag="VNN25_16780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH93241.1"
                     /translation="MFAPEIILRSSKQAVVATDDTEAIVFLNHAAEELYGWRASEVLG
                     KPLAEVLTLGDSLTADSLFEALRTGRSWSGAHRSGPLDGPPSVVLLSGSPLPAEDGNA
                     GGYVLLSMPLDQFELPKIAAESEQLYREIVNATPEGIWILNERDETVFANPQLAAMLG
                     YTQDEMRGAPSSQFVAGDGHAIDAQHGERYRAGHSAQLELTLKRKDGAMLCALLETRP
                     LFRADGSYRGVRARIIDISERRAAEEALRNERKQIREIMKIARIAEWRWHIESDAIEG
                     MPELWEILKVDESAGLTLIDLLTRFIHSDDCDRVSEAFYRTRSENLPLDIEFRLRTNG
                     DTDLVLNMRGYSVMDSTGKTTGLSGVLQDVTERRQLESRLLQAERVSSLGRLAASVAH
                     EFNNVLMGIQPFVDLLNRRAGADPTVALAAPRIADAVARGKRITQEMLRFTRIGEPSR
                     ATIDVRTWLEEFEPELAQLAGPRVKVIVEAAPALMMAADGHQLRQVFANLIVNAQHAM
                     PEGGEVAIRATPDLLRDRSERLNEAVHFTISDNGIGMPEETLRYIFEPLFTTRRFGGT
                     GLGLAVAAQVVQQHKGHIYAESVEGKGSTFHILIPAAQAPKTIAAPEVTTAPSKQEPA
                     REGTRVTLVEDDESVGAGLATILDLEGIAVDWVRLGLDAVGRIAANRPDAVILDLGLP
                     DIDGLEVYREIAKRWPGLPVLFSTGHGDETMLQPVLSAHVAYIQKPYEMQALIDALDK
                     VIQK"
     gene            9823..10620
                     /locus_tag="VNN25_16785"
     CDS             9823..10620
                     /locus_tag="VNN25_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93242.1"
                     /translation="MSRERILIVEDDRAAEIILRLPLEREGFLVDFVEEGYAGIEKLQ
                     AYEYSAVILDLIIHRGLNGFGVLNFIEMEQPKLLNRVFLMTGMSEQTVMNTAPQLLSR
                     LFRKPFSYRALVAAVVAAVRPATSRPNPGEKSLLVVDDDSMAAELVAMFGRRLGMEVT
                     IAENGRRAIEKIDGTAFDTIVLDLLMPGIDGFGVLEFLKRCRPELLSHTIISSGLPEK
                     YRERVAQYSVCGVLPKPIDHRTLQEMIGRCISPPDHIEGGVRQAHSL"
BASE COUNT         1965 a         3289 c         3486 g         2058 t
ORIGIN      
        1 cacgaacgag cgtcaggtca cgcacaacgg cgccgcgaat ttctgtccct actttatgaa
       61 cgatgggaag cgattgatct tcgcgtcgaa cgtgaacaac accggattca acttcgatct
      121 ctggacgatc gggaaggacg ggcagggcct cgagcgcatc acgaccgcgc caggcttcga
      181 cggattctgc gtcttcagcc ccgacggtca gtacctgatc tgggcgtccg ggcgcgccaa
      241 acccgagagt cacgagatga acttgtttat cgcaaagtgg gtggattgag aacgtcggtt
      301 gcggcacgcg aagacgcgca cgcgaagacg cgaaggcgcg aatcggaagt caactgcttc
      361 tttcgcgtct tcgcgtcttc gcgtgccaga ccgagcgcgc tactttttct tcaccgagat
      421 cgtcgccgtc gaactggctc ttgagccgtt gtcgtcgatt gcttcgacgc ggacgtcgcc
      481 gtagacggat gaaaagagac tagtgtcggt gatttctttc tttggattga agtggtagtg
      541 cagctcgcct ttggccggaa tcgaggtggc gaagccgagg gactggattt tgggggcgtc
      601 gtaggattcg ctcgccacgg agatgccgcc gcccgcgtag acgttggcgg tgacgcggtc
      661 gatggtcact ggatggccgc cagtctcctt cacgacgacg tcgaacggga actcatatcc
      721 actgccaccg gtgttgttgg ccagcaccgg gttcggtgtc acggtcagtt cgatggctcc
      781 atgtccgcgc gtggtcttgg tcttcgtcgt cccgggctga cgcgtggcgc atgcgatcgg
      841 aacgaccagg aggaggacga aggccagcct cctcatgtcg tccgcctgac gggaaacatc
      901 atgggcccgt tcactgatgc gacggcgttc gcaagcgtga cgacgccgca gccgatgcgg
      961 gggccggcgt tgcctgcggg ctgcgagacg agatcgtccg gattgccgtg aagtacgacc
     1021 gcgtggccga cgacggcgtt tgtgccggta ttgatcgaga tcgagtgcgt cgtgaagcgc
     1081 gtgtggacct cacctttgtc gtcggcagtt acgttgccga aatcgccggc gtgatgggag
     1141 acggcatcgg gtgcgccgtg caccatgccg ttgggattga agtgaccgcc cgcattctgc
     1201 gcgttgttgc cgcagtcgcc cttctcgtga acatggaatc cgtggacgcc cggaggaacg
     1261 ccggtgaggt cgacgaccac ttccacgccg ttctcgccgg catcctggaa atgaaccgtc
     1321 ccttttgcag tcgacccgct ggtcgaatcg attgtggcgt ttgccatcgg gcctctgctg
     1381 tggccacagg cgaggaagag aaggacgatc ggaactacca gaacttttct catacagcct
     1441 ccgtcttgca aaattcgagc gattctagtg cgaatggggt cttcgtgcgc tttggaagcg
     1501 cggggaggtt ccgttattgc gaccacttgg cgatcttttt ccccagcttt cccagccatc
     1561 ccttcgccac atcctggtag tacatcgacg cggctttgcc gagggcgtcg gcgagggatg
     1621 ggtagggaga gacgaggccc gcgagctggg cgacggtcat ttttttctgc cgcgccagga
     1681 ccagtgtttc gatgagggtt gaggcattgg cgcagagggc atgggcgccg aggatgcggc
     1741 cgcgtttgtc gcagacgagc ttcacgaatc cgcccgtcgt tcgatcgacg accgctcgat
     1801 cgacttcggc catctcgacg ctgtaggtgc gaacgttctc gttgccaagg gcggccgtcg
     1861 cctccgtttc cgtcatgccg atgtggccaa cttccgggtc cgtgtaaaga ccccagggga
     1921 tgttgtcgta gctgacaggc gagcgaccgg ggaagagcat gttgcgcacg agcttcaccg
     1981 cttcgaacgc ggcaacgtgc gtgaattgca tgccgccgtg aacatcgccg caagcccaga
     2041 tgcgaggcac ggacgtctgc agatacttgt cgacggcgac gaagccgcgg ttggttttga
     2101 ttccggcgtc ttcgaggccg aggttttctg tgtttgcgcg gcgtccgctg gcgacgaaga
     2161 tctcgtcgac gcgcagctcc ttcgtttcgc cgagtttgtt ctcgacgcgg acgactttct
     2221 ttcctccatc gagacggacg gacttggcgc cccagccgct gcggatgtcg attccctcat
     2281 cgccgagaat gcggcggagg cgcgcgatca cgtcgtggtc ttctttgttg atgatctcgt
     2341 cgagcatctc aacgacaacg acttcgctgc cgaagcggcg gaacatctgc gcgaactcga
     2401 taccgatgta gccgccgccg aggatcagga gcgatgcagg aaacgaatcc tgattgagga
     2461 aggagacgtg atcgaggaag ccgttctcgg ccagtctttc gaccggcggg atggccgtgc
     2521 gggtgccggt ggcgatgacg atgtctttgg caatgaggcg gcgtccgtcg acgtcgactt
     2581 cctttcggga gatgagcctg gcgttgccga agatgacgtc gatgccgagg gtgcggaact
     2641 tctcaggatc gtcgtgatgc tcgatggcgt gacgctgcgc gcgcatcgag tccatgatgg
     2701 acttcggcgt cacgcgcaca gactgcggtt cgagtccgta ggcatcggca tggcgcaact
     2761 gatgggcgag gcgtgccgag gcgacgagcg ctttcgtcgg aacgcagccg gtccagaggc
     2821 aatcgccgcc aagtttctcg cgctcgatga gagcgacctt gcgcccgagg cgcgcgcagc
     2881 cggaagcgat cacgagtccg gcagtgccgc cgccgatgac aatgacgtcg aagagttcgg
     2941 gcatgtgggt gaatcttatc tgggagtggg aagtgtggag tcgcgagtcg gcgtcctttg
     3001 tcgcgctcag tcttgctcat tctccgacgc ccgaaaactc ccacccccaa caccccacac
     3061 cctacccccc gggattcacc gcacttcata accagcagcg atcagcgcat cgcggatcgg
     3121 gccaggtctg tcggtcgtga tcgtgtggat gccgaggcgg accatgtcga tggcgatcga
     3181 cggctcgtcg acggtccaga cgaccgagcg gagtttctca tcgtgaagcc gtgtgatcag
     3241 agatggtggc acggcgaagc tcgtatcgat gccgttcagg tcgtactgct ttatgagcgc
     3301 gatcaggtcg tcttcgacgt acgttttcgt cacggtgcgg aagccggggc cttcgtcgta
     3361 gagcacggtc cagccgccgt tgttgtagtc ggcttcaaag acgacgataa ggaggcagtc
     3421 gttatcgggc agctcgtgct tcgcggcgat gatcgtgtcg atatcgaagc tgatgagaac
     3481 gacgttcgcc gccttgcccg actgccgcac gatctccgcc gccggcggca cgacctgcgg
     3541 gccgtctttc agctcaatga aaagctcgca ggcatcgtct acggcatggg gcatcgcttc
     3601 gagcacgtct tcgagcagcg gcaagcgcgt tcccgcccac ttctgcgctt tccagcgccc
     3661 ggcgtcgagt ccgcgaagct cgttggcgaa gcggtcacgc accgtccaat tcgcgttcgt
     3721 ggtgcggttc gtcgtgtcgt catgaatcac gatgacctga ccatctttcg tgagatgaac
     3781 gtcgagctcg caggcgccgg cgatcttcca tccctcgctg aacgccggaa gcgtgttctc
     3841 gggcggcgca tctttctgaa cgaagccctg gcccatgccg cgatgcgcga ccaccttcac
     3901 tacctgctta gccattgacg tacaccgtca cctttctggc gggccgcgga ccgtcgagct
     3961 caatgaaata gatgccttgc cacgtgccga ggatcagttg accgttgcga taaggccagg
     4021 agagggatgt gccgatgagc agcgagagga agtgcgcatc ggagttgcct tcgccgtgcg
     4081 cgaagcgaac tttcggaacg agggcgcgca acgctttcgt caggtcggca ggcacgtcgg
     4141 gatcggcgtt ctcattgacg gagatggcgc aggtcgtgtg cggcacgatg atcgtggcga
     4201 tgccgtcgtt gagcgtcagc gcgctgagcg cacgctggac gtcgggtgtg atgagaagga
     4261 tctgctcgcg tgcggtagtg cgaacggtga aggattgctc gcggagcatg ggtgggaatg
     4321 gtagcagggt ggttcgtcgg gagtcgggag tcggagtaag taatcgattg ggccacgtcc
     4381 tgcgcctggc aattactaac tcgcccgatt gcaacgctcc aacccccgac ccccgacaac
     4441 cgactcccac tccccacacc ccggtataat ccagccactc acctatccca tgccattttt
     4501 ctgcatccac tgcaggtcgg aaatcgattc gaagttcaag gcgtgcccgt attgcgggga
     4561 gcccatcacc gactttctcc gccggtatct cgaacatccg atcgacggca agtatcagat
     4621 cctggcgcgc ctcggcgtcg gcgggatggg ggaggtctac aaagtcctcc acctccatct
     4681 gaacgcgatc cgcgtcatca agctgatgcg cgccagcatc gccagcgagc cgggggcgga
     4741 cgatcgcttc atccgcgagg cgcgcctggc cacgcgcatc aatcacccga acgtcgccgc
     4801 gctctatgac ttctccacgc tcgaggacgg ctcgcggtac atggtgtggg agtacatcga
     4861 agggaccaac cttcacgagc tgatcgaaac gaacggtccg ctcacgccgc gctatgccgc
     4921 gaagctggcc atcgaggcgc tgcacggcct cgaagcgatt caccgcgccg gcatcgtcca
     4981 tcgcgacatc agtcccgaga acatcatgat ctgccctgat gaagagggca cgcagcatgt
     5041 gaagctgatc gacctcggca ttgccaagca gggcgacgcc accgacgcga gcaagacggc
     5101 gaccgggatg ttcgtgggca agtggaagta ctgctcgccc gagcacctcg gcatgctcga
     5161 cgccggcgag cggatcgacg gccgcgctga tctctattcg ttcggcatcg tgctgtacga
     5221 gatgctcacc ggtgttcctc cgtttcaagc cgacacgccg cacgcgtatc tgatgctgca
     5281 ctcctcggag aagccgcgcg cgctggccga ggtcaatccg tcgatgccgt cgtcaccggc
     5341 gctcgaagcg ctgatcttca aggcactcga aaaggaccgg aagaaacgtt tcgcctcggc
     5401 acgcgagttc gcgcgtgcga tcgaggcaat cgagccgtcg ctcaacgatc aggttggcgc
     5461 atcgcggccg ttagcgcgag cggtcgaggt gacggaagaa ccgacgaagg tcgcgacgcc
     5521 gacgcagctc agcggcggcg cgcagccgaa agctctcgcc atcgactcca aagcaccgac
     5581 cgagcttacg gatcacggaa agccaatcgc caatcgcgag ctgcgtaaag tatcgacgac
     5641 gaacgtcgtg ccgctgccgc cacgtccgcg cgcacgcatt gcctggtttg ccgcggttgc
     5701 cgtgctgttg gccctcgtca tcgttgcgat cgtcgttcgc gtgaagccgt cgcccgaggt
     5761 cgcgccggtg aaggaaacgc cggcggttac cgcgtcgaat cctgtttcag tggcaccggg
     5821 ccgcgtggcg ttgaacgcgt ttccgtgggg agaggtgacg agcgtccgca acgttgcgac
     5881 agggactgct gtctcgctga aagagcctgt gacgcctcta accctcgagc ttgcaccggg
     5941 cacgtacgag atcacgatgt cgaatcccgg gttcgcgact ccgatcacgc gcaaggtcga
     6001 agtgaaaggc ggcgccgacc agcccgtcaa cattcagttc gccgatcctg cgaccgccac
     6061 gctgccgcga ttcgaggacg tggcgcaatg aagcgggcga tgctcgttct tctctgcctc
     6121 gccgcatggc ctgcgctcgc ggaagagaag tggctcgacg cctacaaccg cggcgtcagc
     6181 gcggtgcggg ccaacaactt tcagcctggc gcgcaggcgc tgcagaaagc gatcgaggag
     6241 atgccgcagg agaacgcggc gcagcgtgtg cgcgatcaga tcttcacgta cgtaccgcac
     6301 ttctggctcg gcattgccag gctgaacctc gccgatcccg atggcgcgct gcgcgagtgg
     6361 aaaacgtccg aggagcaggg ggcgatcaag agcacgccgt attactcgca gcttcaggaa
     6421 ctgattggcc gcgcgaactc ggagaaacag cgccgcgcgc aggtggcggc gaatccggcc
     6481 aagcaggagg cgaacgccgc gatcagccgc gctctgtcgg cgcagatgga cgcggtgacg
     6541 gcaggcggcg atcgaagcga tacgtatcac gccgcccaac ggaagctggg cgaagcgaaa
     6601 gcgttgagcg ccaagggcgg cctcgacgtg cgcgttttca aacatgcgag cgatgtcgcg
     6661 gaagaggcgc gaggtctgtt cgcgaccgcc gcggatgacg caaagaagca gcgcgcggcc
     6721 cggccaccga agaccaatcc ggtgcaggag cgaaagccgc tcgaggttgt ggtcccgttc
     6781 gatgaagggc cgccgccgaa accggtcatt caaccgccgc cgccacacac gacggcaccc
     6841 gtgacgcaga cttccgcgcc gccgcctccg aagacggacg tgaagccgaa ggttgccgag
     6901 ccgccgcccg aggagagtga agcgctcgtt gccgcgcgaa tcgctgtgca gcaataccgc
     6961 cggcggttgc tggcgtcgaa tatgccgacg accgatgcgc agcggttgga tcgccaacta
     7021 acgcctcaaa gcgattcgaa tctcattcga cgcgtcgtcg acgaaatcgc tgcgaaggaa
     7081 cgagagctcg acaagaaggc ggcagcgata ccgggcaagc cggccgagat ccctgcctcg
     7141 gatccgacgg cgggcgagtt ggagtctgcg tatcgagctt acgcggccgg cgacttggcg
     7201 tcatccgacc gaatcctcac aaacgttctt tcctcgaagc cgagcgccga ggcgtatttg
     7261 ctgcggggat gctcgcgata cacgcaggcg atgctctcgc gcgacggtaa cgcgcttctg
     7321 gccagtgcag cctcggacat ccaggccgcc ctccgcatca accattcgtt gcggctggac
     7381 cgcggcgcgt ggtcgccgaa actcatcgcc ttcttcgaga aggtgaagag ccagtaggcg
     7441 ggcacgcgaa gacgcgaaga cgcgaaggtt tgtctttgag gttcttcttc gcgacctgcg
     7501 tggcttcgcg tgagaaccca gcgctgcgct acttctggat cactttatcc agcgcgtcga
     7561 tcagtgcctg catctcgtag ggcttctgga tgtaggcgac atgtgcgctg agcacgggct
     7621 ggagcatcgt ttcgtcgccg tggccggtcg agaagaggac cggcaggccg ggccagcgtt
     7681 tggcgatctc gcggtagacc tcgagtccat cgatgtcggg taggccgagg tcgaggatga
     7741 cggcgtcggg tcgattggcg gcgatgcggc cgacggcatc gagtccgagc cgcacccagt
     7801 cgacggcgat gccttcgaga tcgaggatcg tcgctaaccc ggcgccgacc gactcgtcat
     7861 cttcgaccag cgtgacgcgc gtgccctcgc gtgcaggctc ctgcttcgaa ggcgcggtcg
     7921 taacttctgg tgcggcaatc gtctttggcg cctgcgccgc aggaatgagg atgtggaacg
     7981 tcgatccttt gccttccacg ctttcggcgt agatgtgccc tttgtgctgc tggaccacct
     8041 gcgccgcgac ggccaggccg aggccggtgc cgccgaatct tcgcgttgtg aagagcggct
     8101 cgaagatgta gcgcagcgtc tcttccggca tcccgatgcc gttgtccgag atggtgaagt
     8161 gaacggcttc gttgagccgt tcgctgcggt cccgaagcag atcgggcgtc gcgcggatcg
     8221 caacctctcc accttccggc attgcgtgct gcgcgttcac gatcagattg gcgaagacct
     8281 ggcgaagctg gtgtccatcg gcggccatca tcagcgcggg ggctgcttcg acaatgacct
     8341 tcacacgggg accggcaagc tgagccaact cgggctcgaa ctcctccaac cacgtgcgca
     8401 catcgatcgt ggctcgtgag ggctcgccga tccgcgtgaa gcgaagcatc tcctgggtga
     8461 tccgtttgcc gcgcgccacc gcgtcggcga ttcgcggagc ggccagcgcg accgtgggat
     8521 cagcgccggc gcggcggttc aggagatcga cgaacggctg gattcccatc agaacattgt
     8581 tgaactcgtg cgcgactgac gccgccaggc gcccgaggct cgacacccgt tcggcctgca
     8641 gcaggcggct ttccaactgg cgccgttccg tcacgtcctg caacacgcct gagaggcccg
     8701 tggttttccc agtcgaatcc atcacgctgt atccgcgcat gttcagcacg agatcggtgt
     8761 cgccgttcgt ccgcaggcgg aactcgatat cgagcgggag gttttcgctc cgcgtgcgat
     8821 agaaagcctc gctgacgcgg tcgcaatcat cggaatggat gaagcgcgtc agtagatcga
     8881 tgagcgtgag gccggccgac tcgtcgacct tcaggatctc ccacagctcc ggcataccct
     8941 cgatggcgtc gctttcgatg tgccaccgcc attcggcgat gcgggcgatc ttcatgatct
     9001 cgcggatctg ttttcgctcg ttacgcaggg cttcctcggc ggccctgcgc tcggaaatgt
     9061 cgatgatcct cgcccgcaca cctcgatagg agccgtcggc gcggaacagc gggcgcgttt
     9121 ctagcagcgc gcacagcatc gctccatctt tgcgcttgag ggtcagctcc aactgtgcgg
     9181 agtgccctgc gcggtatcgc tctccgtgct gcgcgtcgat ggcgtggccg tcgccggcga
     9241 cgaactgcga tgagggggcg ccgcgcatct cgtcttgcgt atatccgagc attgccgcga
     9301 gctgcggatt ggcgaagacc gtttcatcgc gctcgttcag aatccagatt ccctcgggag
     9361 tggcattgac gatctcgcga tagagctgtt cgctctcggc agcgattttc ggcagctcga
     9421 actgatcgag cggcatggag agcagaacgt atccgccggc gttgccgtct tccgcgggca
     9481 gcggcgagcc gctcagcaag actacggaag gcggcccgtc gagcggaccg gaacgatgcg
     9541 ctcccgacca cgagcggccc gtacgcagcg cctcgaaaag cgaatccgca gtgagagagt
     9601 cgccgagcgt caacacctcg gcgagtggct tgccgagcac ctcggacgca cgccatccgt
     9661 acaactcctc cgcggcgtga ttgaggaaaa cgatcgcttc cgtgtcatcg gttgcgacga
     9721 cggcctgctt cgaggatctg agaattattt cgggtgcaaa catcaggagc gcctgaatgg
     9781 gctgtccctc gtcgctcgat gcggcatata attatagacg tagtgagcag agaacgcatc
     9841 ctcatcgtcg aagacgaccg tgccgcggaa ataatccttc gacttccact cgagcgggag
     9901 ggcttcctcg tcgacttcgt cgaggaaggc tacgctggga tcgagaagtt gcaggcgtac
     9961 gaatactccg ccgtcattct cgatctgatc atccatcgcg gactgaatgg attcggtgtg
    10021 ctgaacttca tcgagatgga gcagccgaag cttctcaacc gcgtctttct catgacgggg
    10081 atgtcggagc agacggtgat gaataccgcg ccgcaattgc tttcgcggct ctttcgaaaa
    10141 ccgttcagct accgcgcact cgtggctgcc gtcgtcgcgg cggtgcgtcc ggcgacctcg
    10201 cggccgaatc cgggtgagaa gagcttgctg gtcgtcgatg acgacagcat ggccgccgag
    10261 ttggtggcca tgttcggacg gcgcctcggc atggaagtca cgatcgccga gaacggccgc
    10321 cgggccatcg aaaagatcga cggcacggcc ttcgacacga tcgtgctcga tctgctcatg
    10381 cccggaatcg acggcttcgg cgtgctcgag tttctcaagc gctgccgtcc ggagctgctg
    10441 tctcatacga tcatctccag cggccttccc gaaaagtacc gggagcgcgt cgctcaatat
    10501 agtgtttgcg gtgtactgcc caagccgatc gatcaccgca cgctccagga gatgatcggg
    10561 cggtgtattt ccccgcctga ccacatcgaa ggtggcgtga ggcaagcgca cagcttgtga
    10621 cttcgatcgt tcgtctgcat ttcttacgcc tgtagggaat cgatggcggc agcgcgtgtc
    10681 cagcgagctc gttctcagga agaacgtggt tctcgtatcg caaagccccc ctcatccgcc
    10741 ttcggcacct tctccccccg aaaaagcccg gggggagaag gcgctcgatg caaaggag
//
LOCUS       DATLVW010000567         4558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_435248, whole genome shotgun sequence.
ACCESSION   DATLVW010000567 DATLVW010000000
VERSION     DATLVW010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435248"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VNN25_16790"
     CDS             <1..549
                     /locus_tag="VNN25_16790"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXH93243.1"
                     /translation="QQMKQALNRVNQLLGARYGYDPTDDRALSTWDMTKSANVTANIA
                     VGIQLFLGIVGALTLLIGGVGVANIMYAVVKERTREIGVKMALGAKQRWIIAPFVLEA
                     LVYTFIGGALGIIIATILVSLTSFVPIESNKVLSFLGRPTLSPQIGVATSAILGLIGL
                     AAGYFPARRAARIDPASTLRYE"
     gene            566..1270
                     /locus_tag="VNN25_16795"
     CDS             566..1270
                     /locus_tag="VNN25_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015296996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93244.1"
                     /translation="MTTQNLIIRMDDIRKTYDTGKVKVEALKGIHLEIEKGEMIAIVG
                     PSGSGKSTLMNLIGCLDTPTSGIYEIGGQAVSGVTRDELAEIRNRRVGFVFQAFNLLP
                     HITALENVELPMLFGGIKPAERHKRASELLERVGLGDRLDHKPTELSGGQMQRVAIAR
                     ALAMEPDIVLADEPTGNLDSSAGGDIMSIFTDLWQQGRTIVIITHDPALSRRASRVVE
                     IHDGRITSDQATNAAA"
     gene            complement(1410..2141)
                     /locus_tag="VNN25_16800"
     CDS             complement(1410..2141)
                     /locus_tag="VNN25_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXH93245.1"
                     /translation="MTRSGLWRVGELAKRTGVSVRTLHWYEEIGLMSPSQRSEAGHRL
                     YAAEDLGRLQQIRSLRQLGFSLDEIRACLGERQFSPQQVISLHLGRLDQEIALQQRLR
                     ARLESIAARYGAAEAVSAEEFIQAIEAMTMMEKYYTKEQLEELEQRKGVLGADAIKAV
                     EQEWPQLLAKVRTEMENNTDPADPRVQALAKRWMELLQAFSGGNREIEKSAANMYRNE
                     PGVAQQYGLDPRMFPYIQKAIDAGK"
     gene            complement(2257..2628)
                     /locus_tag="VNN25_16805"
     CDS             complement(2257..2628)
                     /locus_tag="VNN25_16805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HXH93246.1"
                     /translation="MGDIEQLFADWAEAVRGKDAEALAGMVTEDCEFWTHGAPALRGR
                     GAVTAAFRKAFATSSFDQTWNEIERLSGDDFIVSVGLEVTQVTPEGGEPIVVTQRGWT
                     LARRCEDGRWRFARGTTNRES"
     gene            complement(2639..2866)
                     /locus_tag="VNN25_16810"
     CDS             complement(2639..2866)
                     /locus_tag="VNN25_16810"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXH93247.1"
                     /translation="MITVHLPEPLREGRGDVMRMNAASLDDIIVRLDLAGDRDELFNF
                     AVNGELILHGEKDVTLKNGDEVEIVVAFAGG"
     gene            complement(2863..>4558)
                     /locus_tag="VNN25_16815"
     CDS             complement(2863..>4558)
                     /locus_tag="VNN25_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXH93248.1"
                     /translation="YFPSFMKLNGIDHLVLYGKAPAWTLLHFEGNEPKFVDAAPWVGL
                     DNLDLRAKIAEDFHGVEGKDYGLAAITAAGENLVLCSGIMAGPKAIYARGGPGAKMGG
                     LHLKAVLIQGRAADLTTARPHKLRNRDVALKLLSTSVVKNALKIRGTPFLYKPSRMLG
                     AMGTKNNQETTWTDKLDAENIDPYRPGMEGCFACPVNCRPLNDMSGVGESDFGGRESE
                     KETGEGFAPTPDPRLPTPAQDKYIKGDGPEYVTLGKFGPNIGITEVTQVLRLNNICND
                     LGLDTASAGSAIAWAMELYQRGIITQEQTKLDLTWGNYDVIEKLLFMIAKREGIGDTI
                     ADSSQAVANGKYPAEALDYLMAVKGLTQSDPHDARILKAFALGLAVATRGMDHLRNRV
                     TLEINAKINDSPDFKAELYGGPVSAEPNSYEGKEIAVRRCENIYAVGDSVGMCRFTTK
                     LFNSPSLPGYPEFVDQVENVTGISLTAEQIDGIGLNVMGVERLINERIGVTRADDTLP
                     KRWFEEAIGVGPYKGEKIDRAEFDKMLSRFYKISNLTEEGVPKPEWREELEGVLAS"
BASE COUNT          834 a         1509 c         1324 g          891 t
ORIGIN      
        1 cagcagatga aacaggcgct gaaccgcgtc aatcaactgc tcggcgcgcg gtacggctac
       61 gacccgaccg acgaccgcgc tctctcgaca tgggacatga ccaagtccgc gaacgtcaca
      121 gccaacatcg ccgtcggcat ccagctcttc ctcggcatcg tcggcgcgct cacgctgctc
      181 atcggcggcg tgggtgttgc caacatcatg tacgccgtcg tcaaagaacg gacgcgcgag
      241 atcggcgtga agatggccct cggcgctaag cagcgctgga tcatcgcccc gttcgtgctc
      301 gaagcgctcg tctacacctt catcggcggc gcgctcggca tcatcatcgc cacgatcctc
      361 gtctcgctga cgagcttcgt gccgatcgag agcaacaaag tcctctcgtt cctcggccgc
      421 ccaacgctgt cgccacaaat cggcgtcgcc acatccgcga tactcggcct gatcggcctc
      481 gccgccggct acttccccgc gagacgcgcg gccagaatcg atccggcgtc gaccttgagg
      541 tatgagtaat cagctcggag accaaatgac cacgcaaaac ctcatcatcc gcatggacga
      601 catccggaaa acgtacgaca ccggcaaggt gaaggtcgaa gccttgaaag gtatccacct
      661 cgaaatcgag aagggcgaga tgatcgctat cgtcggtccg tcaggctccg gcaaatcgac
      721 gctgatgaac cttattggct gccttgacac gccgacgtct ggtatttacg agatcggcgg
      781 ccaggccgtc tccggcgtca cgcgcgacga gctggccgag atccgcaacc gccgcgtcgg
      841 cttcgtcttc caggccttca atcttctgcc gcacatcacc gcgctcgaaa acgtcgagct
      901 gccaatgctc ttcggcggca tcaagcctgc ggaacgccac aagcgtgcct ccgagctcct
      961 cgagagagtc ggactgggcg accgcctcga tcacaaaccg accgagcttt ccggcggcca
     1021 gatgcagcgc gtcgccattg cccgcgctct ggccatggag cccgacatcg tcctcgccga
     1081 cgagcccacc ggcaacctcg attcatccgc cggcggcgac atcatgtcga tcttcaccga
     1141 cctctggcag caaggccgca cgatcgtgat catcacccac gaccccgccc tctcccgccg
     1201 cgccagccgc gtcgtcgaga tccacgacgg caggatcacg tccgatcaag ccacgaacgc
     1261 cgctgcatag aacggcggcg ttccaacgca aaacgttgcg ttcccgaata aacgcaactc
     1321 ccgaatcgag ccccttctcc ccatcgcttt tcagatgggg agaaggtggc cgaaggccgg
     1381 atgaggggcc ctcacatcag aaacaagaac tacttccccg cgtcgatcgc cttctgaatg
     1441 tacgggaaca tcctcggatc gagcccgtac tgctgcgcca cgcccggctc attccggtac
     1501 atgttggccg cgctcttctc gatctcgcga ttgccgccgc tgaaggcttg cagcaactcc
     1561 atccaccgtt tcgccagcgc ctgcacccga ggatctgcgg gatcggtgtt gttctccatc
     1621 tccgtgcgga ccttggccag gagctgcggc cactcctgct cgaccgcttt gatcgcatcg
     1681 gcaccgagca cgcctttccg ctgctcgagc tcttccaatt gttctttcgt gtagtacttt
     1741 tccatcatcg tcattgcctc gattgcttga atgaattctt cggcggagac agcctccgcc
     1801 gcgccatacc gcgccgcgat cgactcaaga cgggcgcgca gccgttgctg caacgcgatc
     1861 tcctgatcga gccgcccgag gtggagagag atcacctgct gcggtgaaaa ctgccgttca
     1921 ccgaggcacg cacggatctc gtcgagcgag aatccgagct gcctcagcga ccggatctgc
     1981 tgcaggcggc cgagatcttc agccgcatac agccggtgcc ccgcctccga gcgttgcgat
     2041 ggcgacatca ggccgatctc ttcgtaccag tgcaaggtcc gaacagaaac gccggtccgc
     2101 ttcgccagct cgcccacccg ccagaggccg gagcgcgtca tgcaacctcc tcaaaatcgt
     2161 gatcgttcgt gcgcacgaac gacaagatag aacctcacgt tacgtcagag gcaagaggta
     2221 ttttggggct gggttcctgg tgcacgtagc tgcccgtcat gactcgcggt tcgtcgtgcc
     2281 gcgagcgaag cgccagcgtc cgtcctcgca ccgccgcgcc agggtccagc cgcgttgtgt
     2341 gacgacgatt ggctcgccgc cctcgggagt cacctgagtc acctcgaggc caacactaac
     2401 gatgaaatca tcgccgctga ggcgctcgat ctcgttccac gtctgatcga acgagctggt
     2461 cgcgaacgcc ttgcgaaacg ctgccgtgac cgcaccgcgg ccgcgcagtg ccggtgcgcc
     2521 atgcgtccaa aactcgcaat cctccgtcac catcccggcc aacgcctcgg cgtccttccc
     2581 gcgaactgcc tcggcccagt cagcaaacag ttgttcaatg tcgcccatga atgcccgcct
     2641 atccgccggc gaaggcgacg acgatctcca cctcatcgcc attcttcaac gtgacgtcct
     2701 tctcaccgtg aaggatcaac tcgccattga ccgcgaagtt gaacagctcg tcgcgatcgc
     2761 cggccaggtc gagtcggacg atgatgtcgt cgagcgatgc ggcgttcatc ctcatgacat
     2821 cgccccggcc ttcgcgcaat ggttccggaa ggtggacggt gatcatgacg ccagcacgcc
     2881 ttcgagctcc tcgcgccatt ccggtttcgg cacaccttcc tcggtgagat ttgagatctt
     2941 gtagaagcgg gagagcatct tgtcgaactc ggcgcggtcg atcttttcgc ctttgtatgg
     3001 cccgacgccg attgcttcct cgaaccagcg tttgggcagc gtgtcgtccg cgcgggtgac
     3061 gccgattctc tcgttgatca ggcgctccac acccattacg ttgaggccga tgccgtcgat
     3121 ctgttcggcg gtcagcgaga tgccggtgac gttttcaacc tgatcgacga actcgggata
     3181 gccgggaagt gacggcgagt tgaagagctt cgtcgtgaag cggcacatac cgaccgaatc
     3241 gccgaccgcg tagatgttct cgcagcggcg gacggcgatc tcttttcctt cgtagctgtt
     3301 cggctccgcg gagaccggac cgccgtacag ctcggctttg aagtcggggg agtcgttgat
     3361 cttggcgttg atctcgagcg tgacacggtt ccgcaggtgg tccatgccac gcgtcgcgac
     3421 ggcaaggccg agggcgaagg ctttgaggat acgggcatcg tggggatcgc tctgcgtcag
     3481 gcctttcacc gccatcaagt aatcgagcgc ctcggctgga tacttgccgt tggccaccgc
     3541 ctgcgaggaa tcggcgatcg tgtctccgat gccctcgcgc ttcgcgatca tgaagaggag
     3601 cttctcgatg acgtcgtagt tgccccacgt cagatcgagc ttcgtctgtt cctgcgtgat
     3661 gatcccgcgt tggtaaagct ccatcgccca ggcaatcgcc gagccggccg atgctgtatc
     3721 gagcccgagg tcgttgcaga tgttgttgag acgaaggacc tgtgtgactt cggtgatgcc
     3781 gatgttcgga ccgaacttgc cgagggtgac gtattcgggg ccgtcgcctt tgatgtattt
     3841 gtcttgcgcg ggagtcggga gtcgggggtc gggggtcgga gcaaaacctt cgcccgtttc
     3901 tttctccgac tcccgacccc cgaaatccga ctccccaaca ccggacatat cgttcagcgg
     3961 ccggcaattc acgggacagg cgaagcagcc ttccatcccg ggtcgatacg gatcgatatt
     4021 ctcggcatca agcttgtcgg tccaagtcgt ctcctgattg ttctttgtcc ccatcgctcc
     4081 gagcatgcgc gacggcttgt agaggaacgg cgtgccgcgg atcttcagtg cattcttcac
     4141 gaccgaggtc gagagcagct tcagcgcaac atcgcggttg cgcagtttgt gcgggcgggc
     4201 ggtggtgaga tcggccgcac gtccctgaat taggactgcc ttgagatgca gcccgcccat
     4261 cttcgcgccc ggtccgccgc gcgcgtagat cgctttcgga ccggccatga tcccggagca
     4321 gagcacgagg ttctcgccgg ctgcggtgat ggccgccaga ccgtagtcct tcccttcgac
     4381 accgtgaaag tcttcggcga tcttcgcgcg caggtcgagg ttgtcgagtc cgacccacgg
     4441 cgcggcatcg acgaacttgg gctcattccc ctcgaaatga agcagcgtcc aggccggcgc
     4501 cttcccgtag aggacgaggt gatcgatgcc gttgagcttc atgaacgacg ggaagtag
//
LOCUS       DATLVW010000568         3436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_394564, whole genome shotgun sequence.
ACCESSION   DATLVW010000568 DATLVW010000000
VERSION     DATLVW010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394564"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNN25_16820"
     CDS             <1..508
                     /locus_tag="VNN25_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXH93249.1"
                     /translation="ERLRGYRHAMRGLETAALRALEFQGAFTEESGFAAGQKIASALP
                     RPTAVFAANDSMAVGALAAFAAAGIRVPETMSVIGFDDIPIARYVAPPLTTMRVDIAE
                     LGRRAFALLLDIIINPTAHTARRERVATTLVVRGSCAAAKSVSTRQTRPHRAARVPGS
                     GREKGEVS"
     gene            505..3321
                     /locus_tag="VNN25_16825"
     CDS             505..3321
                     /locus_tag="VNN25_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH93250.1"
                     /translation="MRRTFSFIVGVALLMAAIALPAAAQLTTATIRGKVTNANGTALP
                     AAEIDAVNAASGFVKTVQSGPDGSFTMAGISPGEYNLVIAAPGFQARNETVRVLVGQN
                     IEMNFVMSPDAVMNQNITVVGNQLVETRTSEAATNVTPQQIESLPQGDRNFLNFAQLA
                     PGIRLSTDPLRKTIAGDAQPAEQTNVFIDGVSQKNDVLQGGLVGQDSSRGNPFPQNAV
                     QEFRVITQNFAAQYDHASSAIISAVTKSGGNAFSGNAFYYYQPKQWVAATAKGFQFST
                     LSTNASYHRSQPGISFGGPIIKDKLNFFVSYEGVDEHATTIVNTLNTPKLPEAGGAFP
                     SPFKSNLAFGKLSWQPDKNQLLDISGNYRREHETRDFGGTTSFQSATDLRNWVYGATM
                     RHQWNNASALNQGSLSWQDYGWNPTPLDSSSVGLDYQGIGRVGGNSTTQKFDQRRIEL
                     RDDFNFAPMQWNGEHAFQIGGNFDDMHYLVNKSLNGNPQFEFRNDPANGFTYAQPFEA
                     NFGFGNPILKTNNREYGIYGQDSWTVNPKLSFSLGLRWDYESHMLDESYVTPANIVAG
                     LSGKIDPSYFSNGNQRKPAKDEFQPRLGFTYDLTGKNETIVFGGYGKYYDRLFLNATL
                     DERYRLQYPVYRIEFSPDGRAGTIKWNDSYFTQAGLNGLIASGQTHPEIYLLNNKTKV
                     PYSNMFNLGIRQVLGPWLGSVSYNGVRGYHGFTWLSAAPNGLCCAALVPGFGNVIKSD
                     PEGKRYWYNAFEAKLDKPFTKQARWGATFAWTHGRAEQTGNDLFSLDFPSAAAYGKHD
                     VPGSERDRITATGIFGLPYDIQFSAIASFGSGQETNVLDFTQGFSLENREATHPFSRS
                     ITPPKKWGFADRNVDFRAQKDFATMGRTSIGIVGAVYNAFNWTNYGCLNNFLGPGDNG
                     SSLGIPNCVIRLGRREEVGLRVNF"
BASE COUNT          713 a         1106 c          991 g          626 t
ORIGIN      
        1 cgaacggttg cgcggctacc ggcacgccat gcgcggcctg gagactgcgg cgctgcgcgc
       61 gctggagttc cagggcgcgt tcaccgaaga gtcgggattc gcggcggggc aaaagattgc
      121 gtccgcgctg ccgcggccga ctgcggtctt cgccgccaac gactcgatgg cggtcggcgc
      181 gcttgcagcc ttcgccgctg ccggcatccg cgtgccggaa acgatgagcg tcatcggatt
      241 cgacgacatt ccgatcgcgc gctacgtggc gccgccgctg acgacgatgc gtgtcgatat
      301 cgccgagctg ggccggcgcg cattcgctct tctgctcgac atcatcatca atcccaccgc
      361 acacacggcg cggcgcgagc gcgtggccac aacgctcgtg gtacgcggat cgtgcgcggc
      421 ggcaaaaagt gtatccacaa gacagacgcg gccgcatcgt gcggcccgcg ttccgggaag
      481 tggccgagaa aaaggagagg tgtcatgaga cgaaccttta gtttcatagt tggagtggct
      541 ttgctgatgg ccgcgatcgc attaccggct gccgcacagt tgacgacggc cacgattcgc
      601 ggaaaagtga ccaacgcgaa cgggacggcg ttgcctgccg cggaaatcga cgcggtcaat
      661 gccgcaagcg gcttcgtgaa gacggtgcag tccggaccgg acggctcgtt cacgatggcc
      721 ggcatcagcc cgggcgaata caacctcgtg atcgccgctc ccgggtttca ggcgcgcaac
      781 gagaccgttc gtgttctggt cggccagaac atcgagatga acttcgtgat gagtcccgat
      841 gcggtgatga accagaacat caccgtcgtc ggtaaccagc tcgtcgaaac gcggacctcg
      901 gaagcagcga cgaacgtcac gccgcagcag atcgagagcc tgccgcaggg cgaccgcaac
      961 ttcctcaact tcgcccagct ggcaccgggc atccgtctgt cgacggatcc actgcgcaag
     1021 acgatcgcag gcgacgccca gccggccgag cagacgaacg tcttcatcga tggcgtcagt
     1081 cagaagaacg acgtcctgca gggcggcctg gtcggacagg actccagccg cggcaatccg
     1141 ttcccgcaaa acgccgtcca ggagttccgc gtcatcacgc agaacttcgc agcacagtat
     1201 gaccacgcat ccagcgccat catcagtgcc gtgacgaaat cgggtggcaa tgcgttctcc
     1261 ggcaacgcct tctattacta ccagccgaaa cagtgggtgg ccgcaacggc gaagggcttt
     1321 cagttctcga cgctgtccac caacgcctcg tatcaccgct cgcagcccgg catcagcttt
     1381 ggtggcccga tcatcaaaga caagctcaat ttcttcgtat cgtatgaagg cgtcgacgaa
     1441 cacgccacga ccatcgtcaa tacgctcaac acccccaagc tccccgaggc cggcggcgcg
     1501 ttcccgagcc cgttcaaatc gaacctggcg ttcggaaaac tgagctggca gccggacaag
     1561 aatcagctcc tcgacatctc cggcaattac cgccgcgagc atgaaacgcg cgatttcggc
     1621 ggtacgacgt cattccagtc ggcgaccgat cttcgcaact gggtttatgg cgcgactatg
     1681 cgacaccagt ggaacaacgc cagcgcgctc aaccagggct cgctgagctg gcaggattac
     1741 ggttggaacc cgacgccgct cgattccagc tcggtcggtc tcgactacca gggaatcggc
     1801 cgcgtcggcg gcaacagcac gacgcagaaa ttcgaccagc gccgcatcga gctgcgcgac
     1861 gatttcaact tcgccccgat gcagtggaac ggtgagcacg ctttccagat cggcggcaac
     1921 ttcgacgaca tgcattacct cgtcaataag agcctcaacg gcaacccgca gttcgagttc
     1981 cgcaacgatc ccgccaacgg gttcacgtat gcgcagccgt tcgaagcgaa tttcggtttc
     2041 ggcaatccca tcctcaagac gaacaaccgc gagtacggca tttacgggca ggatagctgg
     2101 accgtcaatc cgaaactctc cttcagcctc ggcctgcgat gggattacga gtcgcacatg
     2161 ctggacgaga gttacgtcac gcccgcgaac atcgtcgccg gcctttccgg caagatcgat
     2221 ccgagctact tctccaatgg caatcagcgc aagccggcca aggacgaatt ccagccccgt
     2281 cttggcttca cgtacgatct gaccggcaaa aacgagacga tcgtcttcgg tggctacggc
     2341 aagtattacg accgcctctt cctcaatgcc acgctcgacg agcgctatcg tctccaatat
     2401 ccggtctatc gcatcgagtt ctctcccgac ggccgcgccg ggacgatcaa gtggaatgat
     2461 tcatacttta cgcaggccgg tctcaatgga ctgattgctt cgggtcagac gcatcccgag
     2521 atttatctcc tcaacaacaa gaccaaggtc ccgtactcga acatgttcaa cctcggcatc
     2581 cgtcaggtgc tgggaccgtg gcttggatca gtcagctaca acggcgttcg cggatatcac
     2641 ggcttcacat ggctgtcggc cgcgccaaat ggtctctgtt gcgccgcgct ggtgccggga
     2701 ttcggcaacg tcatcaagtc tgatccggaa ggaaaacgct actggtataa cgcctttgag
     2761 gcgaaactcg acaagccgtt cacgaagcag gctcgttggg gcgccacctt tgcgtggacc
     2821 catggccgcg cggagcagac cggcaacgat ctcttcagcc tcgatttccc gagtgcagcc
     2881 gcatatggca agcatgacgt cccaggctcc gagcgcgacc gcattaccgc gaccggcatc
     2941 ttcggtctgc cgtacgacat tcagttcagc gccattgcgt ccttcggctc cggccaggaa
     3001 accaacgtgc tcgacttcac gcagggattc agcctcgaga atcgcgaagc cacacatccc
     3061 ttctcgcgct cgatcacgcc gcccaagaag tggggcttcg ccgatcgtaa tgtcgatttc
     3121 cgagcgcaga aagatttcgc cacgatgggc cgcacgtcca tcggtatcgt cggcgcggtc
     3181 tataacgcat tcaactggac caactacggc tgcctcaaca acttcctcgg accgggagac
     3241 aacggcagca gcctcggcat cccgaattgc gtcatccgtc tcggacgcag ggaggaagtc
     3301 ggcctgcgcg tgaatttcta aactgaggtt tgtggggagt ggggtgtggg gatcgggaga
     3361 acgcaatttg caagcgctct tccctttctc atgctcccac tccctgtgac ccgctcacgt
     3421 tcgctcttgc gcatgc
//
LOCUS       DATLVW010000569         8295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_107155, whole genome shotgun sequence.
ACCESSION   DATLVW010000569 DATLVW010000000
VERSION     DATLVW010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107155"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VNN25_16830"
     CDS             complement(<1..166)
                     /locus_tag="VNN25_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXH93251.1"
                     /translation="MTSSGDDSVTRKREAKRWPVQDAKARFSELLDASLTSGPQIVTR
                     RGEEMAVLVRV"
     gene            814..2052
                     /locus_tag="VNN25_16835"
     CDS             814..2052
                     /locus_tag="VNN25_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXH93252.1"
                     /translation="MTIDTGNTGFMLLCSSLVMLMTPGLAFFYGGLVGRRNVLSIMMQ
                     SFVSMGWTTVLWWAFGFSMCFSGDLKSGTDFHGIIGNFHWAFLSGITLDTPSAINTSI
                     PMIVFCAYQMMFAIITPALITGAFANRVTFKAYMLFLTFWLIFVYFPFVHMIWGGGIL
                     ASWGVLDFAGGIVVHNIAGIAALASVLYVGRRKVEDRGPHSIPLVALGTGLLWFGWYG
                     FNAGSEFRVDSVTAVAFLNTDIAASFGAIAWLCLDWMQFRKPRFVGLLTGAVAGLATI
                     TPAAGYVSPATACLIGLIAGVVCYYAVALKNKLRWDDALDVWGVHGVGGFLGIVLCGV
                     FATTAFNPAGVDGLLRGNSHFFLVELAAVAISSAWAFVFTYGMLWLIDRITPVKVLDT
                     AEVEGLDQALHGETAYIEGI"
     gene            2136..2771
                     /locus_tag="VNN25_16840"
     CDS             2136..2771
                     /locus_tag="VNN25_16840"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH93253.1"
                     /translation="MRKYELGGVTVQKRSRLPHWHAEHAIYFVTWRLFDSLPVHVLER
                     LRAERDATREQISRMRGSVTFPEQRMLDAALTEACENFLDQNVGECYLRDHRAAQIVA
                     DALNHFDGSRYLLFAWSVMPNHVHAVFSCVEGWDTSRILHSLKGFTSREINKILGRSG
                     TLWQAESYDRCVRDSEELERTIDYVLGNPAACGMENWPFTAMYPARIGEAT"
     gene            complement(3510..5411)
                     /locus_tag="VNN25_16845"
     CDS             complement(3510..5411)
                     /locus_tag="VNN25_16845"
                     /inference="COORDINATES: protein motif:HMM:NF020693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol oxidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXH93254.1"
                     /translation="MSNGSTPPGWPTGIPIVQEQYQNWSRGITVPNVWTCTPATPADV
                     VTVCNWAAGANFQIRPRGIMHGWSPLTVPNGEPVANVVLVDLTVNLNQVLAITSAANG
                     QPAQVKVQTGMVMEDLMTALQNAPGGSTTTPGTGYGFAHIPAPGHLTVGGVLAINAHG
                     TAVPNQGANNLDISYGSLSNQILAFTAVVSDPSSGSPNSYVAKTFSRTDTDALAFLTH
                     LGRAFLLDVTLQLTNNYNLRCQSIMTIDSSVLFAPQTPGSPPPAQSCGDFLNQSGRIE
                     IIWFPVFPTVTWKTYPWLKVWTVSPKQPSGSKLVNGPYNYPFSDNLPLGVTDAVDAMV
                     SLAPGLTPLFTGITALFTAFALNPLGSLAADKKAASSVATSTSLAAVAEKQSPGITDA
                     ISQLTSAVPGLAGYFAEMAKYAWNPFNLTDLWGASMNTLLYVKDTTLRVTANGYAIQM
                     NSADVQNAIAAFTTQFTDMLAEYQQKSPALYPINSPMEIRVTALDEFPPGQTTLSPII
                     SALSYDDVAKKNQWDVAVWFDVLTVQPDGDPQQANAFYTELEAWISNHFGNAQQYRIN
                     PEWSKGWAYTTQNGAWTNAEYLERIRENFTTGRTTSPTWADEVAALAKYDAANLFWNP
                     FLQELFVNP"
     gene            5840..7843
                     /locus_tag="VNN25_16850"
     CDS             5840..7843
                     /locus_tag="VNN25_16850"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH93255.1"
                     /translation="MGKRAFERVVVIIFENEFRGSVMQNSYMRSLAAKGIELANYFGV
                     MHPSNTNYVASVAGEICNISSDPLYYTFLPEPQGAPAPAPGPEPMPQKTIADVIVENG
                     LEWRAYMAGYSPVAFPPSLAPILDPSSGQIDVAATVSQTILDYPPYLNMHNPFVRFSS
                     IVNDENQWKRIGTSHDFLRDALDGTLPQYSWFTPNMWDCGHWLYGTYNEPAQRAPFLV
                     DQLAKWLESFFGVLAFPGPGSRMPKGTLVVVTFDEADMNKSYEEIRNWDSTYDGPNQI
                     YTVLLGDTITPQRIEQEGYNHYSLLRTVEKNFDLPALGTNDTDANWFQFLWGKTYAWG
                     APEPTPIENATTVAAASLGDTLYAVYGSGGALSCRTRTNGTWSPAQDVAGPQNVTTVA
                     MASANGNLTLICQSGGALSALSLSNGAWSAPQQVATGVQAFSLVSYLDYGDQTEKLML
                     AWSSDSGLQSQIFANGQWTTPVAVGQQGGGAVVLATLGASVFLIHQAGGTSLMNVASY
                     NTAPFNVVTSAGDSNTTQYAWSPSVYPVQHFAAGPTRAPGGDDSLAPYQGAAPFAAAS
                     LDGVIHFIHRAAKDSILCSTTFSISGVLTPQNPVSYKQSGTGLSNGYGTLAEAGWSRR
                     AQVSGLPVEGSSAMALARAGRDLVLLWQERSGSPLHLTSGRYR"
     gene            complement(7893..7991)
                     /locus_tag="VNN25_16855"
                     /pseudo
     CDS             complement(7893..7991)
                     /locus_tag="VNN25_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
     gene            8091..>8295
                     /locus_tag="VNN25_16860"
     CDS             8091..>8295
                     /locus_tag="VNN25_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93256.1"
                     /translation="MKTETDRFETVAHRLKTETDCFETVAHRLKTETDRFETVAHPLK
                     TETGPFRNGGAPFENRDGPFRNGG"
BASE COUNT         1511 a         2607 c         2553 g         1624 t
ORIGIN      
        1 cgacgcgtac aagaacagcc atctcctctc cgcgccgagt cacaatctgc gggccgcttg
       61 tgaggctggc atcgagcaat tcactgaacc gtgctttggc gtcctgcacc ggccagcgtt
      121 tcgcctctct ctttcgagtc actgaatcgt ctcctgagct agtcatctga ctagtcagca
      181 ctcatcatag ctctacgcga atggaaatgt cgcgcctgaa ttcagtatcg ttgggcggcg
      241 caattcttga tgacttcaca ttcagcgaat cgagttatga aggccctcgc cttcgcgatc
      301 atcgcgtacg tttcggcgtc ggcggtcgcc tgccgccagc ccacgtttcg ggagcagcac
      361 ttcgaacgtg ctcgacagcg tttgcttgct cgcatggagc aagatcgtgt gacccgtgcg
      421 cggtttgaaa aactcggacc gggaacaccg gcggtcaaga tcgtgaagga gctcgggaag
      481 ccaaatacca gaactccttg caacaacgcg cacgagtgct ggttctacga catttcgggt
      541 ccgacctact tcgtatgttt cgacgcgcac gccacggtca cttgcgaagg gaccgcatcc
      601 gtttttcgac caagccctgc ccagacaact ccggccaact aggagtctgc gcggctgaaa
      661 ctactttcga cctcccccaa actgccctcg ggcgaggacg cccgagggcc cgcggtcgag
      721 acgaccgcac tccttgcgat gcacctcaat ccgttcgccg aaatctcccg gccggtgcca
      781 tcatgggcat ctcgatagga gacgtccatg cacatgacga ttgatacggg caataccggc
      841 ttcatgctcc tctgctccag cctcgtcatg ctcatgacac ccgggctggc gtttttttat
      901 ggaggcctgg tcggccgccg caatgttctg tcgatcatga tgcagagctt cgtctccatg
      961 ggttggacga cggtactctg gtgggccttc ggcttctcga tgtgtttcag cggcgatctg
     1021 aagagcggaa ccgatttcca cggcatcatc ggcaacttcc actgggcctt cctctcgggc
     1081 atcacgctcg acaccccctc ggccatcaac acttcgatcc cgatgatcgt cttctgcgcc
     1141 taccaaatga tgttcgccat catcacgccg gccctcatta cgggcgcctt cgccaatcgc
     1201 gtgacgttca aggcctacat gctcttcctc accttctggc tgatcttcgt ctacttccca
     1261 ttcgtacaca tgatctgggg tggtggaatc ctggcctcct ggggcgtcct cgacttcgcg
     1321 ggtggaatcg tggtgcataa catcgcagga atcgcagccc tcgcctccgt tctctacgtc
     1381 ggcagacgca aagtcgaaga ccgcggaccg cactcaatcc cgctcgtggc actcggcaca
     1441 ggtctgctct ggttcggatg gtacggcttc aacgcaggaa gcgagttccg cgtcgactcg
     1501 gtaaccgccg tcgcgttcct caacactgac atcgccgcca gcttcggcgc aatcgcgtgg
     1561 ctctgtctcg attggatgca gttccgcaag ccgcgctttg tcgggcttct gaccggcgcc
     1621 gtcgccggcc tcgctaccat cacgcccgcg gccggatatg tctcgcctgc taccgcctgc
     1681 ctcatcggac tcattgccgg cgtggtctgc tattacgcgg ttgcgctgaa gaacaagctc
     1741 cgctgggacg acgcgctcga cgtctggggt gttcacggcg tgggtggctt cctcggcatc
     1801 gtcctctgcg gcgtcttcgc aacgaccgcg ttcaatccag ccggcgtcga cggcctcctg
     1861 cgcggaaact cccacttctt cctcgtggag ctggccgccg tagcgatctc ctcggcatgg
     1921 gcattcgtct tcacctacgg catgctttgg ctcatcgacc ggatcacacc ggtgaaagtc
     1981 ctcgacaccg ccgaagtcga aggcctcgac caagccctgc acggcgagac ggcgtacatc
     2041 gaagggatct gacgtagtgc aggcgtccgt gcctgcatcc gccgggcgtc cctgcccggc
     2101 ggtgttgttc cgcagagccg aggtatcctc gggcgatgcg gaagtacgaa ctgggcggag
     2161 ttacggttca aaagcgatct cggctgcctc actggcacgc tgagcacgcg atttacttcg
     2221 tcacctggcg gctgttcgat tcattgccag ttcacgtcct ggagcgactt cgggctgagc
     2281 gcgacgccac ccgcgaacag atttcgcgca tgcggggaag cgttacgttt cctgagcaga
     2341 gaatgcttga cgcggcactc accgaggctt gcgagaactt tctcgatcag aacgtcggcg
     2401 aatgctatct ccgcgatcac cgagccgcgc agatcgtcgc cgacgccctg aatcatttcg
     2461 atggcagccg ctacctcctt tttgcgtggt cagtgatgcc caatcacgtt cacgctgttt
     2521 tttcttgcgt cgaaggatgg gataccagca ggatccttca ctcgctcaaa ggattcacga
     2581 gcagggagat caacaagatc ctcggcagga gcggcacgct ttggcaagcg gaaagctacg
     2641 atcgatgtgt ccgcgactcg gaggagctgg aacgaacgat cgactatgtc ctcggcaacc
     2701 ccgccgcgtg cggcatggag aactggccct tcaccgccat gtacccggcc agaatcggcg
     2761 aggccacgta gtgcaggtgt ccacacctgc tcccgacggg cgtccctgcc cgtcggtgtt
     2821 ttttgcgccc gacagccagc cgaaggtacg ttttgtcatt ccacttcgaa aaccaccgtc
     2881 gggcgaggac gcccggcggc tgcaggcaag aatgcctgca ctacgccgcc atgtacccag
     2941 acagagtcgg cgaagccacg tagtgcaggt gtccacacct gcatccgacg ggcgtccctg
     3001 cccgtcggtg gtttttgcgc cgacagccag cggatacgtg ttgtcgttcc acttcgaaaa
     3061 ccaccgccgg gcgaggaggc ccggcggctg caggcaagaa tgcctgcact acgtcgccat
     3121 gtacccggcc agaatcggcg aagccacgta gtgcaggtgt ccacacctgc tcccgacggg
     3181 cgtccctgcc cgtcggtggt ttttgcgcag acagccagcg gaaggtacgt tttgtcattc
     3241 ctcttcgaaa accaccgccg ggcgaggacg cccggcggct gcaggcaaga atgcctgcac
     3301 tacgccgcca tgtacccggc cagaatcggc gaagccacgt agtgcaggtg tccacacctg
     3361 ctcccgacgg gcgtccctgc ccgtcggtgg tttttgagca gacagccagc ggaacgtatg
     3421 tgttgtcatt ccacttcgaa aaccaccgcc gggcgaggac gcccggcggc tgcaggcaag
     3481 aatgcctgca ctacgtcttc tgggacgaat cagggattga cgaagagctc ctgcaggaac
     3541 gggttccaga aaaggttcgc ggcatcgtac tttgcgagcg cggccacctc gtccgcccag
     3601 gtgggcgacg tcgtgcggcc ggtcgtgaag ttctcgcgga tccgttccag gtactctgcg
     3661 ttggtccagg cgccgttctg ggtggtgtac gcccatccct tcgaccactc cgggttgatg
     3721 cggtactgct gggcgttgcc gaagtggttg ctgatccacg cctcgagctc cgtgtagaac
     3781 gcgtttgcct gctgcggatc tccgtcgggc tgcacggtca ggacgtcgaa ccacaccgcc
     3841 acatcccact gattcttctt cgccacatcg tcgtagctga gggcggagat gatcgggctg
     3901 agcgtcgtct ggccgggcgg gaactcatcg agcgcggtga cgcggatctc catcggcgag
     3961 ttgatgggat agagagccgg cgacttctgc tgatactcgg ccagcatgtc cgtgaactga
     4021 gtagtaaagg cggcaatggc attctgcacg tccgcgctgt tcatctggat ggcgtacccg
     4081 ttcgccgtga cccgcagcgt ggtgtccttg acgtagagga gcgtgttcat cgacgcgccc
     4141 cagagatcgg tgaggttgaa cggattccac gcgtatttcg ccatctcggc gaagtagccg
     4201 gcgaggcccg ggacggcgct ggtcagttgg ctgatggcgt ccgtgattcc cggactctgc
     4261 ttttccgcga ccgcagcgag ggacgttgac gtcgcaacgc tcgaggcagc cttcttatcc
     4321 gcggccagcg aaccgagggg attgagggcg aatgcggtga agagggcggt gatgccggtg
     4381 aagagcggcg tcagccccgg cgccagcgag accatggcgt cgaccgcgtc ggtgaccccg
     4441 agcggcagat tgtccgagaa cggatagttg tacgggccgt tcaccaactt ggagccggac
     4501 ggctgctttg gactgactgt ccagactttc agccacggat acgtcttcca ggtgacggtg
     4561 gggaagaccg ggaaccagat gatctcgatg cggccggact gattgaggaa gtcgccacag
     4621 ctctgcgccg gcggcgggga gcccggcgtc tgcggcgcga acagcacgct gctgtcgatg
     4681 gtcatgatgc tctggcagcg caggttgtag ttgttggtca actgcagggt gacgtcgagc
     4741 aggaacgcac ggcccaggtg cgtgaggaag gccagggcgt ccgtgtcggt gcggctgaac
     4801 gtcttcgcca cgtaggagtt gggactgccg gaggagggat cgctgaccac cgctgtgaag
     4861 gccaggatct gattgctcag cgagccatag ctgatgtcga ggttgttggc gccctggttc
     4921 ggaacggccg tgccgtgagc gttgatggcc agcacgccac ccacggtgag atgtccgggc
     4981 gcggggatgt gagcgaagcc gtagccggtg ccgggggtag tggtgctgcc gcccggcgca
     5041 ttctgcaacg cggtcatgag gtcttccatg accatgccgg tctgcacctt cacctgcgcc
     5101 ggctggccgt tggcggcgga ggtgatcgcc aggacctggt tcagattcac ggtcaggtcg
     5161 accaggacta cgttggccac cggctcgccg ttcgggacgg tcagcggcga ccagccgtgc
     5221 atgatgccgc gcgggcgaat ctgaaagttc gcgccagccg cccagttgca aaccgtcacg
     5281 acgtcagccg gggtcgccgg cgtgcacgtc cagacgttcg gtacggtgat gccgcgcgac
     5341 cagttctgat actgctcctg cacgatgggg ataccggtag gccatcccgg gggtgtcgat
     5401 ccattgctca tagctctctc cctctgcgtg atcccggcgc cgccaacgtc gggcgttccg
     5461 gtcatcaagg cgatgaccgc gaagcctcgc tggcctgtgc gggaaacgct gcgtattgta
     5521 ccgggagtgg atcgttactt acggcgaaga atcagaaggt ctcgttgatc accggcgcga
     5581 gatgcaaaga ctccaaagta gtggggcaat ttgaaaaacc ggtgaccgca gagacacgga
     5641 ggtcacagag atcccctctg tgttctctgt gtctctgtgg ttgccttttg cttcggcgac
     5701 cacgataggc cgtccggttg acccacgacc agactccacc tcatgatcgc gattcgcagc
     5761 ggtagggtgt aatgttttca caaaatcatt gactgcctga cggaattgcg gagcagccgt
     5821 atggtggtaa ggagtggaaa tgggcaagcg cgcattcgaa cgtgtcgtgg tgatcatctt
     5881 cgagaacgag ttccgtggct ccgtgatgca gaactcctac atgcgctcgc tcgccgcgaa
     5941 ggggatcgag ctcgccaact acttcggcgt gatgcacccc tccaacacga attacgtcgc
     6001 ctcggtggcg ggcgagatct gcaacatctc cagcgatcct ctgtactaca cgtttctgcc
     6061 ggagccccag ggagcgccgg cgcccgcgcc cggacctgaa ccgatgccgc agaagacgat
     6121 cgccgatgtg atcgtcgaga atggcctcga gtggcgcgcc tacatggccg gctattcgcc
     6181 cgtcgccttc ccgccgagcc ttgcgccgat cctcgacccc tcatccgggc agatcgatgt
     6241 ggcggcaacc gtcagtcaga cgatcctcga ctaccccccc tacctcaata tgcacaatcc
     6301 gttcgtccgc ttcagcagca tcgtgaacga tgagaaccag tggaagcgga tcggcacgtc
     6361 ccacgacttc ctgcgcgacg cactcgacgg cacactgccg cagtactcgt ggttcactcc
     6421 caacatgtgg gactgcgggc actggcttta cggaacgtac aacgaaccgg cccagcgcgc
     6481 gccgtttctc gtcgatcagc tcgcgaaatg gctcgagtcg tttttcggcg tgctcgcctt
     6541 ccccggcccc ggctcccgca tgccgaaagg aacgctggtc gtcgtcacgt tcgacgaggc
     6601 cgacatgaac aaatcctatg aggagatccg caactgggac agcacctacg acggtccaaa
     6661 ccagatctac accgtcctcc tcggcgacac gatcactccg cagcgcatcg agcaagaggg
     6721 atacaaccac tacagccttc tgcggaccgt cgaaaagaac ttcgatcttc cagcgctcgg
     6781 gacgaacgac acagacgcga actggtttca gtttctctgg gggaagacct atgcctgggg
     6841 cgcaccggag ccgacgccga tcgagaacgc cacgaccgtt gccgccgcct cgctcggcga
     6901 cacgctttat gccgtttacg gatcgggcgg cgcgctttcc tgccgcaccc gaacgaacgg
     6961 gacgtggtcg ccggcgcagg acgtggccgg tccgcagaat gtcaccacgg tggcaatggc
     7021 ttccgccaac gggaatctca ccctgatctg ccagtcgggc ggcgcgctgt cggcgttgtc
     7081 gctgagcaac ggcgcatggt ctgcgccgca gcaggtcgcc accggagtgc aggcgttctc
     7141 gctcgtctca tacctcgact acggcgatca aacggagaag ctcatgctcg cgtggagcag
     7201 cgatagcggc ttgcagtcgc agatcttcgc taatggccag tggacgacgc cggtcgcagt
     7261 cggacagcaa ggcggcggtg ccgtcgtcct cgccacactc ggtgcgtcgg tcttcctgat
     7321 ccaccaggcg ggtggaacga gcttgatgaa tgtcgcctct tacaacaccg caccgttcaa
     7381 cgtcgtcacg agcgccgggg acagcaacac gacgcagtat gcgtggtcac catccgtgta
     7441 tccggtccag cacttcgctg ctggtccgac acgcgctccg ggcggcgatg actcactcgc
     7501 accgtatcag ggagcggccc cctttgccgc ggcttcgctc gacggcgtga tccatttcat
     7561 ccaccgcgca gcgaaggact cgatcctgtg ctcgacgacg ttttcgatca gcggcgttct
     7621 cacgccccag aatccggtga gctacaaaca atcagggacg ggactgagca acggatacgg
     7681 aacgctggcc gaggcgggat ggagccggcg agcccaggtt tcaggcctcc ccgtcgaagg
     7741 cagcagcgcg atggcgttgg cccgcgccgg ccgcgacctc gtgctgttgt ggcaggagcg
     7801 cagcggctct ccgttgcact tgacctctgg ccgctaccgg tgaagggatc taagcagcga
     7861 cgcaaccgac gggcgtccct gcccgtcggt gttgcgaagc gagcggtgag gccgtcggac
     7921 atgccgatga cgacgtctcg taccgtgtcg ctggccgtga aatgacgttc ggtgcggatc
     7981 gttcgcggca tgggcgcgcg ccttgtgcgc gcgcgggggt gcagacatct cgcgcttcgt
     8041 ctccgcctgc ggtcccaggc ggtctgcgtt tcaatacgca aacgcaccct ttgaaaacgg
     8101 agacggaccg tttcgaaacg gtagcgcatc gtttgaaaac agagacggac tgtttcgaaa
     8161 cggtggcgca ccgtttgaaa acagagacgg atcgtttcga aacggtggcg caccctttga
     8221 aaacggagac gggaccgttt cgaaacggcg gcgcaccgtt tgaaaacaga gacggaccgt
     8281 ttcgaaacgg tggcg
//
LOCUS       DATLVW010000570         7394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_107178, whole genome shotgun sequence.
ACCESSION   DATLVW010000570 DATLVW010000000
VERSION     DATLVW010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107178"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3904
                     /locus_tag="VNN25_16865"
     CDS             <1..3904
                     /locus_tag="VNN25_16865"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXH93257.1"
                     /translation="VVLLDEAHYSFEANGKAHTTQRHIYRIVDESAIDELGTLEVPWA
                     PWYNDRPTVAARIVSKDGTVHTLDSKAIMESPAREDLDIFSDNRVLRAPLPGVAVGSV
                     IEYVIEFNGNNPIQEAGASDIVVFGGYAPTQRTRVTIDGPSTIEPRIVNKTDVQAKIE
                     EKDGRRIMTFETGRIEGRKDFENFLPFDDISLPYLAFSTGSSWGNLAKRYSEIVDKQI
                     AGDDLKKFTHDAIGNATARREVIARALAAVEKNVRYAGVEVGESSIIPRPPHTVLGNK
                     YGDCKDKATLLVAMLRQAGIVAHVALLRAGVDFDVHPDLPGLGRFNHAIVRADATSSE
                     PAMWVDPTDDFAHAGDLPAQDQGRLALVAAGDTSSLTKTPETPSTENRYSETRTYTLP
                     EDGKAHVTEVTETVSGPEDSFQRHFYAESDRTKYKEQIESYVKTYYSAKKLEKIEATD
                     PHDLTKPFRVTIEASDADTGVARDGDAAVAFHPANLINWLPFPMRNFGDDHAEEARRK
                     RVGDFIFPAAEVREWTYHIVPPAGYNARTLPPNETIKLGTTTLTKEFASKPDGSVTAV
                     LRFDSGKRRISAAEFEDTRAALKKIVEGKPTIIGFDLAGQAKLNAGDVAGALTEFRKL
                     AALHPKEAQHHMEIARALLVGGLGDAAREEIARAVALEPSNAHVQAGLAGILEYDLLG
                     RLMRKGFDLDGSIAALRKAKELDPKNAEYRGALAKVLTFNSEGTEYGPGSKIDEAIAE
                     YRAVAKEAGEKEAKQFDGDLMLALAHAGRFAEMKELAATVQDPQLRETGRIAAIAATD
                     GAAPALHELGAFDANTRRAYAQAIAQTLLNLRLYPQATEMFDAATQGAPNASSARPFI
                     EELKKTKRIEEVPIDEKDPGSVVQKMIIAMTHEDVTALKKLFAFDLKLDDEDEDDLQR
                     SMRSMTFGTGMAPAVVGDISFASLRIQTDGDEKTGYRLRMRGENGMSIPTLFVVHQNG
                     GYVIRASRAMADTIGAAVLALADAGQLDAARTWLNWLREDVPAGGGDDPLSGAPFASL
                     WSKDNTSTNADEIRTAAASTMGHKGTHKSIPILLAAREKASNDKTKTWIDLALSYSYA
                     NKKEWKNVVPITERLRAAYPDSESAFTSNVYALSLSGRTADAEALAKSRLAKKPKDDE
                     VLRALSTNAAKAKDYAAAAKYAEQIVDELSPTQNDYNNAAWFELFVGNVTHAMENARR
                     ATAEESQTSPAALHTLATLYAETGKNIEARDALLRTLDKSRRDTPDSSDWYVLGRMAE
                     NYGVREAALAAYKRVDKKETDGASVWELAQRRLTAMSK"
     gene            4026..4724
                     /locus_tag="VNN25_16870"
     CDS             4026..4724
                     /locus_tag="VNN25_16870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH93258.1"
                     /translation="MFPTTRRSVIVALGSDDDEQRRRAFDTFVAIYWKPLYKYLRVTD
                     RLRETDAEDLTQAFLAKCFENGSLASYDAAKATFRTFLRMLFDRFVTNEVKASQRQKR
                     GGGAITLDFAHAEEEIAREHDRGGSPEDYFQREWVRSVLALAVERLRDACAASGRQTH
                     FAIFEAYDLDETSRASYRETGERFGISESMVTNWLAATRRRFRGIVLDVLREATASDA
                     EYRNEVRALLGVDA"
     gene            4721..5605
                     /locus_tag="VNN25_16875"
     CDS             4721..5605
                     /locus_tag="VNN25_16875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH93259.1"
                     /translation="MTWLSDSAVAHLRMVAEQPDFSATRYRIQRELARGGMGVVYEAE
                     DTELHRHVAIKVLASELASADAVERMRAEARTIARLEHPGIVPLHDVGILPDGRLWYA
                     MKLVRGRRLDELEAQPLELLRVFQRICEAVAFAHANGVVHCDLKPENVMLGDFGEVLV
                     MDWGVAREIGATAGVVAGTRGYMAPEQESGIALDAAADVFALGAMLRALLPAKLPKPL
                     AAICAKATAPGPHDRYPSARDLAADVTLWLDNKPVTAYRENVIERINRWLVRNRALVT
                     IVVAYLLMRVTIVIWFGR"
     gene            5662..6519
                     /locus_tag="VNN25_16880"
     CDS             5662..6519
                     /locus_tag="VNN25_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93260.1"
                     /translation="MNKILVGLLLGAGLGAVDGMTAWFTPTVRAGIIGIVIGSTFKGM
                     IAGIAAGWFARKVKSVAWGIAFGLLVGALLAYGVAAMQHGYYLEIMLPGSIVGAIVGW
                     ATQRYGRPANGSAAASTLAAMFLVFLTVNAHAAEPVKAADAFEHLKSLAGTWNGHAMT
                     PDGPPTSVSYRLTAGGTAVMETLFGGTPHEMLTLYTLNGDDVVATHFCGADNQPVMKL
                     DKAKSSPDRLVFAFVSIDGEHAKDHPHVHDGFIHFAGADQMDASWAMQTPDGSAFEHS
                     PKFFLTRAK"
     gene            complement(6529..6777)
                     /locus_tag="VNN25_16885"
     CDS             complement(6529..6777)
                     /locus_tag="VNN25_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93261.1"
                     /translation="MQQLLAHRFARQLQRMGKLRDRGWALPFESKQDGPAAVRKLVDG
                     DDGLAPLAAASVRGKPEGNIEVRSRDCQVPGCKSFMFV"
     gene            6757..7350
                     /locus_tag="VNN25_16890"
     CDS             6757..7350
                     /locus_tag="VNN25_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HXH93262.1"
                     /translation="MRQQLLQLQREGWIESRVARGDERGRTGRPATRYNLTEAGDHLF
                     PKSYDLLNVAVIDAVAQELGPDAAVRVLKRVCDDRVSIEEKTLQGMPLESRVEALKGL
                     YAPADPYMTSEKVDDGFLLVERNCPFFNTAMRRPTLCSVSVNALTRLLGVRVERDETF
                     QSGAGRCVFHIYANEPIDAKEWDFTLEDELPKHGVRT"
BASE COUNT         1494 a         2315 c         2316 g         1269 t
ORIGIN      
        1 cgtcgttttg cttgatgaag cgcactactc gttcgaagca aacggcaagg cgcatacgac
       61 gcagcgacac atctaccgaa tcgtcgacga gtcggcgatc gatgagttgg gcacgctcga
      121 agtcccctgg gcgccctggt acaacgaccg gcccacggtc gcggcgcgca tcgtctccaa
      181 ggacggcacg gtccatacgc tcgattccaa ggcgatcatg gagtcgccgg cgcgcgagga
      241 cctcgatatc ttcagcgaca accgcgtgct tcgtgcgccg ctgccgggcg tcgcggtcgg
      301 ctccgtgatc gaatatgtca tcgagttcaa cggcaacaat ccgattcagg aggctggggc
      361 atcggacatt gttgttttcg gtgggtatgc gccgacacag cgcactcgcg taacgatcga
      421 cggtccttcg acgatcgagc cgcgcatcgt aaacaagacg gatgtgcaag cgaagatcga
      481 agagaaagac ggccgccgca tcatgacgtt cgaaacggga cgcatcgagg gccgcaagga
      541 cttcgagaac ttccttccgt tcgacgatat ctcgttgccg tacctcgcct tctcgaccgg
      601 ctcgtcgtgg gggaaccttg caaagcgcta cagcgagatc gtcgacaagc agattgccgg
      661 cgacgatctc aagaaattca cgcacgatgc gatcggcaac gccacggcta ggcgtgaggt
      721 gattgcgcga gcgctggcgg cggtggagaa gaacgtccgg tacgccggag tcgaagtcgg
      781 cgagtcctcc atcatcccgc gtccaccgca cacagtcctg ggcaacaaat acggcgactg
      841 caaagataag gcgacgctgc tcgtggcgat gctgcgtcag gcgggaatcg ttgcccatgt
      901 ggcgctgctc cgtgccggcg tcgacttcga cgtccatcct gaccttcccg gcctcgggcg
      961 cttcaatcac gccatcgtcc gcgccgacgc gacgagcagc gagccagcga tgtgggtcga
     1021 tccgaccgac gacttcgccc acgctgggga tctgccggcg caggatcagg gacgtctcgc
     1081 tctcgtcgcc gcaggcgaca catcgtcatt gacgaagacg ccggagactc cgtcgacgga
     1141 gaatcgctat tcggagacgc gaacatacac gttgccggaa gacggcaaag cgcacgtcac
     1201 cgaagtcacg gagacggtaa gcggccccga ggactcgttc cagcgccact tctacgccga
     1261 gagcgatcgc accaagtaca aagagcagat cgagtcgtac gtcaagactt actactccgc
     1321 gaagaaactg gagaagatcg aagccaccga tccgcacgat ctcaccaagc cattccgcgt
     1381 gacgatcgag gcctcggacg cggacaccgg cgtcgcgcgt gacggcgatg cagccgtcgc
     1441 gttccatccg gcgaatctca tcaactggct tcctttcccg atgcgcaatt tcggcgacga
     1501 tcacgccgaa gaggcgcgca ggaagcgagt gggcgacttc atctttcctg cggcggaagt
     1561 gcgcgagtgg acctatcaca tcgttccgcc ggccggatac aacgcgcgga cgctgccgcc
     1621 gaacgagacg atcaaactcg gcaccaccac tctgaccaag gagttcgcat cgaagccgga
     1681 cggctcagtc acggccgtcc ttcgtttcga cagcggaaag cggcgcatca gcgcggcaga
     1741 attcgaagac acacgcgcgg ccctaaagaa gatcgtcgaa ggaaagccga cgatcatcgg
     1801 attcgatctc gctggccagg ccaaactgaa tgcaggtgac gttgccggtg cgctgaccga
     1861 gttccgcaag ctcgctgcgc ttcatccgaa ggaagcgcag caccacatgg agatcgcccg
     1921 tgcgctcctg gtcggcggac tcggtgacgc ggcacgcgag gagatcgcgc gcgccgtcgc
     1981 gctcgagcct tcgaatgcgc atgtgcaggc tggactggcg ggcatcctcg agtacgacct
     2041 gctcgggcgc ctgatgcgta aaggcttcga cctcgacggc tcaatcgcgg cgcttcgcaa
     2101 agcgaaggag ctcgatccga agaatgccga gtatcgcggc gctctggcca aagtgctcac
     2161 gttcaacagc gagggaacgg aatatggacc cggctcgaaa atcgatgaag cgatcgctga
     2221 gtatcgtgcg gttgcaaagg aggccggtga gaaggaagcg aagcaattcg acggcgatct
     2281 gatgctcgct ctcgcgcacg ccggccggtt tgcggaaatg aaagagctgg cggcgactgt
     2341 gcaggatccg cagcttcgcg agacgggccg catcgccgcg atcgctgcga cggatggcgc
     2401 tgcgccagcg ctgcacgagc tgggcgcatt cgatgcgaat acgcgccgcg cctacgcgca
     2461 ggcgatcgcg cagactctgc tcaatctccg gctttacccg caggcgaccg agatgttcga
     2521 cgccgctaca caaggcgccc ctaatgcgtc gagcgcacgt ccgttcatcg aagagttgaa
     2581 gaaaacaaag cgcatcgaag aggttccgat cgacgagaaa gatccaggct ccgtcgtaca
     2641 gaagatgatc atcgccatga cgcatgagga cgtgacggcg ctgaagaagc tcttcgcctt
     2701 cgatctgaaa ttggacgacg aggacgaaga cgatcttcaa cgttcgatgc gttcgatgac
     2761 ttttggcacc ggaatggcgc ccgcggtagt cggcgacatc tcatttgctt cgctgcgcat
     2821 ccagaccgac ggcgacgaga agaccggcta ccggctgcgg atgcgcggcg agaacggcat
     2881 gtcgattccc acgctcttcg tggttcatca gaacggtgga tacgtaattc gggccagccg
     2941 cgccatggcg gacacgatcg gcgccgccgt cctcgcactt gccgatgccg gccagttgga
     3001 tgccgcgcgc acgtggctca actggctgcg cgaagatgtg ccggcgggcg gcggtgatga
     3061 tccgctctcc ggggcgccgt ttgcgtcgct ttggtccaaa gacaatacct cgacgaacgc
     3121 cgacgagatt cgcacagctg ccgcatcgac catggggcac aaaggcaccc acaagagcat
     3181 ccccattctc ctggcggcgc gcgagaaagc atcgaacgac aagacgaaga cgtggatcga
     3241 cctcgcgctt tcgtactcgt acgcgaacaa gaaggaatgg aagaacgtcg ttccgattac
     3301 cgagcgtctt cgtgcggcct atcccgactc ggagagcgct ttcacctcga atgtctacgc
     3361 gctctcgctc agcgggcgga ctgcggacgc cgaggcgctg gcgaagagcc gcctggcaaa
     3421 gaagccgaag gatgatgagg ttctgcgcgc gctttccacc aacgcggcca aggcaaagga
     3481 ctacgcggcc gccgcgaagt acgccgaaca gatcgtcgat gagctctcgc cgacgcagaa
     3541 cgactacaac aacgccgcct ggttcgagct gttcgtcggc aacgtcacgc acgcgatgga
     3601 gaacgcgcgc cgagccaccg cggaggagtc gcagacgtcg cccgccgcac tgcacacgct
     3661 ggcaacgctg tacgcggaga ccggcaagaa catcgaagcg cgcgatgctc tgttacgtac
     3721 gctcgacaag agccgccgcg acacgcccga cagcagcgac tggtacgtcc tcggccgcat
     3781 ggcggagaac tacggggtgc gcgaagcggc gctcgcggcc tacaaacgtg tcgacaagaa
     3841 agagaccgac ggcgcctcgg tgtgggagct cgcgcagcgg cgactgacgg cgatgagcaa
     3901 gtagcgcgct taacacgggg gcacgttttc acgcggagag cgcggaggtt ctctccgcgt
     3961 ccccgcgttc cccgcgtgaa acggttttaa cgtgcctagg accgcgctac cctatcgcct
     4021 ggtccatgtt cccaactact cgacgcagtg tgatcgttgc cctcggcagc gacgacgatg
     4081 agcagcgccg ccgtgccttc gacaccttcg tcgccatcta ctggaagcct ctctacaaat
     4141 accttcgcgt aaccgatcgc ctcagggaaa ctgacgccga ggatctcacg caggcgtttc
     4201 tggcgaagtg tttcgagaat ggctcgctcg caagctatga cgctgcgaaa gcgaccttcc
     4261 ggacgttcct tcgcatgttg ttcgatcgct tcgtcacgaa cgaagtcaaa gcgtcgcaac
     4321 ggcagaagcg cggcggtggg gcgatcacgc tcgacttcgc ccacgccgag gaggagattg
     4381 cgcgcgagca cgatcgcggt ggatcgcccg aggactactt tcagcgcgaa tgggtacgca
     4441 gcgtcctcgc gcttgccgtc gagcggctgc gcgacgcatg cgccgccagc ggcaggcaga
     4501 cgcattttgc aatcttcgaa gcgtacgatc tcgatgagac gtcacgcgcg tcatatcgag
     4561 agaccggcga acggtttggt atcagcgagt cgatggtcac gaactggctc gcagcaacgc
     4621 gccgccgttt ccgcggcatt gtgctcgacg tgctacgcga agcaacggcc tcggacgccg
     4681 aataccgcaa tgaagtgcgc gcactgctcg gagtcgatgc atgacgtggc tttcggatag
     4741 cgccgtcgcg cacctacgca tggttgcgga gcagccagat ttcagcgcca cgcgctatcg
     4801 tatccagcgc gagctggccc gaggggggat gggcgtggtc tacgaggcgg aagacacgga
     4861 gctgcatcgc cacgtcgcca tcaaagttct cgccagcgag ctcgcctcgg ctgacgccgt
     4921 cgaacgaatg cgcgccgagg cgaggacgat cgcgcggctg gaacaccctg gcatcgtgcc
     4981 gctgcacgac gtcggcattc ttcccgacgg ccggctctgg tacgccatga agctcgtgcg
     5041 cggacgcagg ctcgatgagc ttgaggcgca accgttggag ttgctgcgcg ttttccaacg
     5101 catctgcgaa gccgtcgcat tcgcgcacgc caatggtgtc gtccattgcg atctcaaacc
     5161 ggagaacgtc atgctcggcg acttcggcga agtgctggtg atggattggg gtgtcgcgcg
     5221 cgagatcggt gcgaccgccg gagtcgtagc gggaacgcga ggctatatgg cgcccgaaca
     5281 ggagagcggc attgctcttg atgctgcagc cgatgtcttc gccctcggcg cgatgttgcg
     5341 cgcattgctt cccgcgaagc tgccgaagcc cttggccgcg atctgtgcga aggccaccgc
     5401 gccaggtcca cacgatcggt atccgtcggc gcgcgatctc gctgctgacg tgacactgtg
     5461 gctcgacaac aagccggtca ccgcgtaccg cgagaatgtc atcgagcgaa tcaaccgctg
     5521 gctcgtccgc aatcgggccc tcgttaccat cgtcgtcgct tatcttctga tgcgggtgac
     5581 catcgtgatc tggtttgggc gctgacgccg ttaaaggatt ttcaccagac gttgaataga
     5641 ctcactggag gaacaaacga catgaacaaa atcctggttg gacttctcct cggcgccggt
     5701 ttgggcgcgg tcgacggcat gacggcgtgg ttcacgccta cggtgcgcgc cggcatcatc
     5761 ggcatcgtca tcggctcgac gttcaaggga atgattgccg gcatcgccgc cggatggttt
     5821 gcgcggaagg tgaagtcggt ggcttggggc attgccttcg gcctgctcgt cggagccctg
     5881 ctggcgtacg gagtagcggc gatgcagcac ggctactacc ttgagatcat gctgcctgga
     5941 tcgatcgtcg gcgcaatcgt cggctgggct acgcagcgat atggacgccc cgccaacggc
     6001 agcgcggccg cgtcaacgct cgcggcgatg ttcctcgtgt tcctcaccgt aaacgcccac
     6061 gcggcggaac cggtgaaggc ggccgatgcc ttcgagcatc tgaagtcgct ggccggaacg
     6121 tggaacggac atgcgatgac gccagacgga ccgccgacga gcgtcagtta ccgcctcact
     6181 gctggtggca cagcggtcat ggagacgctc ttcggcggca ctccgcacga gatgctgacg
     6241 ctctacacgc tcaacggcga cgacgtggtg gccacacact tctgcggcgc cgacaatcag
     6301 ccggtcatga agctggacaa ggcgaaatcg tcaccggacc gtctcgtttt cgcgtttgtc
     6361 agcatcgacg gcgagcacgc gaaggaccat ccgcatgtac acgacggatt cattcacttc
     6421 gcgggcgccg atcagatgga cgcctcgtgg gctatgcaga ctcccgacgg cagcgcgttc
     6481 gagcacagcc caaagttctt cctgacgcgg gcgaagtgag ctcgcagatc aaacaaacat
     6541 gaatgatttg caacccggca cttgacaatc ccttgatcgg acttcaatgt ttccctctgg
     6601 ctttccgcgc accgatgcgg cggccagagg agccaaccca tcatcaccgt cgaccagctt
     6661 cctgacagcc gccgggccat cctgcttgct ctcaaacggc agggcccatc cacgatcgcg
     6721 cagcttgccc atccgctgca actgacgggc gaagcggtgc gccagcagtt gctgcaactc
     6781 cagcgcgagg gatggatcga gtctcgcgtc gcgcgcggcg atgagcgcgg ccgcaccgga
     6841 cgtccggcca cgcgctacaa cctcaccgag gccggcgatc atctctttcc gaagagctat
     6901 gacctgctca atgtcgccgt gatcgacgcg gtggctcagg agctcggacc cgatgccgcg
     6961 gttcgcgtgc tcaaacgcgt ctgcgatgac cgcgtttcga tcgaagagaa gacgctccag
     7021 gggatgccgt tggaatcgcg tgtcgaagcg ctgaaagggc tttacgctcc cgccgatccg
     7081 tacatgacca gcgagaaagt cgacgacggc tttctgctcg tcgagcggaa ctgccccttc
     7141 ttcaacaccg ccatgcgccg ccccacgcta tgcagcgttt ccgtcaacgc cttaacccgc
     7201 cttctcggtg tccgcgtcga gcgcgacgag accttccaat ccggcgccgg ccgctgcgtc
     7261 ttccacatct acgcgaacga gccgatcgac gcgaaggagt gggatttcac gctggaggat
     7321 gagctaccga agcacggggt tcggacgtag gtttcgataa aatcaaaaag cattgtcacg
     7381 cggagacacg gagg
//
LOCUS       DATLVW010000571         5235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_358755, whole genome shotgun sequence.
ACCESSION   DATLVW010000571 DATLVW010000000
VERSION     DATLVW010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358755"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VNN25_16895"
     CDS             complement(<1..781)
                     /locus_tag="VNN25_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXH93263.1"
                     /translation="MYPWRRIVIPTDFSTAAEWSFDSAVQIAGTTGAELIILHVRATR
                     PSNPDELRFPADDSLYEYAEQHELEKLRDHARRANARVPTRLVIQRAPDPGAAICSTA
                     IEEGADLMVISTHARHHVAHLIIGSTTLKVLSGASLPVLAIRYGIPSRRGLQKILVPV
                     HLKQRSTAAAALASSIARRENSEIHLIIVCEEPERIASQDLLDAMTGSTFDGARVLPV
                     VLQGKDVQAELARYAKTVDADALFLNVGAHEISTLKQDIIRK"
     gene            complement(820..1491)
                     /locus_tag="VNN25_16900"
     CDS             complement(820..1491)
                     /locus_tag="VNN25_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93264.1"
                     /translation="MKRAAYPLLFDGDMTGRRATAVASLLIAGALFVRCTGGQESKAA
                     EEQAPRKNGITVTASTAAASETPQIDAKAALRAINPAIPIYAGAKYRDDLTRRDSVMI
                     KNQYGPNAEVYTLATDDSFPQVYHYYTTYLAQFRAYPTQSPYPATQTWRTLEVQLNQA
                     MQDPFIPGDMLKPGDRQITLQIAETEAEPKTVIRYVITPQPIQSAQAQTPKPAAPSTN
                     AFDRE"
     gene            complement(1488..1997)
                     /locus_tag="VNN25_16905"
     CDS             complement(1488..1997)
                     /locus_tag="VNN25_16905"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HXH93265.1"
                     /translation="MPAKREKEFNTACQEEVYRQVKSYLDELVDEHFDDAEHCDFYLK
                     YGSTVLEISIDPYQEDDAVIEVLAFCVQGVEPSFELMQELLRVNAEVPLGAFSMQGES
                     VFFSHSFLGRRLRPEQLIASLDVVANVSDEYDEKLVASYGGETAIDCLRSGGMRPPVA
                     FAARKADSN"
     gene            complement(2082..>5235)
                     /locus_tag="VNN25_16910"
     CDS             complement(2082..>5235)
                     /locus_tag="VNN25_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HXH93266.1"
                     /translation="VEGAPNTWVITPNANFVAAEQCTVTILQNSVHDRDTDDSAPRTD
                     TLPADYSWTFTVSSGTLPPYPPSVHLTMGQPSICTTSLSSPNAYLMLKPENSICYNRD
                     LGRPNWVSWHLSDEWVGSLTRVDTFRPDPEVPPTWYRVLASDFFASGFDRGHMTPNAD
                     RDKETSIPINQATFLMSNMVAQAPDNNQGPWANLENYLRSLLSVDELYIVSGPAGTGG
                     TGSNGGVTNTVANGHVTVPASTWKCALVLPKASGDDVARVTASTRTICVIMPNIQGIR
                     NDDWHIYLKSVDQVEALTGYDLFSNVPPAIQNAIEAGVDGTNPPGAADQSTSTNEDVQ
                     KSITLDAATPGGALTYTILTGPFHGGLTGTGANQTYTPAPDFNGTDTFTWRVNDGTNN
                     SNTATMTITVAEVNDPPSAVDDSKTATANVALTFASSDLTANDSAGPANEAGQTLTVT
                     SVTPTGSTHGTVSLTSGQITYTPAAGYSGPASFTYTVCDNGTTAGSADSLCATATVNV
                     TVNPPPPATHFSVTAPANVTNGVAFNVTVSALSGSNTAATGYTGTVHFTSSSSGTLPS
                     DYTFTAGDLGAHTFSVTLTSNGSRTITATDTVTASITGTANTNVLAATTHFSVTAPGT
                     FAPGTPFNVTVTALDASNATVTTYAGTAHFTTSAGSSTLPSDYTFVAGDNGVHTFSVT
                     LNASGAQTVTATDTVTASITGSASTTGSCPPVAAPTASNSGPGCAGGTVNLFVSGSGA
                     SYSWSGPGGFTSTLQNPTGVTQPGTYTVTVTTSGACGGSIAVSTTVVFNPLPSATITN
                     GGTACPLSTLNHASVADAGAGASYNWTITNGTITGGIGTRNILYTAGNSGNVHLAVTV
                     TTASTCSASSTADVAITSGPTISVPANLSACGSSTVTIPFTLTGNGPWTVQWSDGLTQ
                     TVIASPASRTISVTTSTTLTVGNVTDSSCTNSSPGATTFIGVTAPPTIATQPQGQIVN
                     PGNNATFTVVANGANLHYQWFVHHASGVTNAVGTDSPSYTTNPEGNATWSVRVSNSCG
                     SIDSVAVNALVVTPRHHPAH"
BASE COUNT          859 a         1491 c         1841 g         1044 t
ORIGIN      
        1 ccttccggat gatgtcctgc ttcagcgtgc tgatctcgtg cgctccgacg ttgaggaaca
       61 gcgcgtcggc gtccacggtc ttggcgtagc gggccagctc cgcttggaca tccttcccct
      121 gcaggacgac cggcaggacc cgcgctccat cgaacgtgct gcccgtcatc gcgtcaagaa
      181 gatcctgcga ggcaatccgc tccggctcct cacagacgat gatcagatgg atctcggagt
      241 tttcacgccg cgcgatcgac gaagccagcg ccgccgccgc ggtggaacgc tgcttcagat
      301 ggaccggcac caggatcttt tgcaatcctc ggcggctggg gatgccatag cggatggcca
      361 gcacaggcag cgaggcaccc gacaagacct tgagcgtcgt cgatccgatg atgagatggg
      421 cgacatggtg acgcgcatga gtcgagatga ccatgagatc tgcgccttct tcgatggcgg
      481 tgctgcagat cgccgcgccc gggtctggag cgcgctggat gacaagacgc gtcggaaccc
      541 ttgcattggc ccggcgcgcg tggtcgcgaa gtttctcgag ctcgtgttgc tcggcgtact
      601 catagagcga gtcgtcagct ggaaagcgga gctcgtccgg gtttgaggga cgtgtcgccc
      661 gcacgtgaag aatgatgagc tcggcgccgg tcgtgccggc gatctgcacc gccgaatcga
      721 acgaccattc agcagccgtc gagaaatcag tcgggatgac gatgcggcgc cacgggtaca
      781 taagatcctc gcacaggccg gacttcagcc tcctgcgctc tactcccgat cgaacgcgtt
      841 tgttgaaggg gcggcgggct tcggggtctg cgcctgggca gactgaatcg gctgcggggt
      901 gatcacgtaa cggatgaccg tcttcggctc cgcctccgtc tcggcgatct gcaacgtgat
      961 ctgacggtcg cccggcttca gcatgtcgcc cggaatgaag ggatcctgca tggcctggtt
     1021 gagctgaacc tcgagagttc gccacgtctg cgtagcgggg tagggcgact gcgtgggata
     1081 ggcccgaaac tgggcaagat aggtggtgta gtagtggtag acctgcggga acgagtcgtc
     1141 cgtcgccagc gtgtacactt cggcgttcgg cccgtactgg ttcttgatca tgacggagtc
     1201 gcggcgggtg agatcgtcgc gatacttcgc tccggcatag atggggatcg cagggttgat
     1261 cgccctaagc gcggccttgg cgtcgatctg gggtgtttct gaagccgcgg cggtgcttgc
     1321 ggttacggtg attccgtttt tccgaggcgc ctgctcttcg gcggccttac tctcctgacc
     1381 gccggtgcac cggacgaata gagcccctgc gatcaggagg gatgcgactg cggtagcccg
     1441 gcgcccggtc atatcaccat caaacagcaa gggatatgcc gctcgcttca attgctatcc
     1501 gcctttcgtg cagcgaacgc aaccggtgga cgcatgccgc cgctgcgaag acagtcgatc
     1561 gctgtctcgc ccccgtagct ggccacgagc ttttcgtcgt actcgtcgga aacgttggcg
     1621 acgacgtcga gggatgcgat gagttgttcc ggacggagac ggcggccgag gaatgagtgc
     1681 gagaagaaga cgctctcgcc ctgcatgctg aacgcgccga ggggaacctc ggcgttgact
     1741 cgcagcagtt cctgcatcag ctcgaaggac ggctccactc cctggacgca gaaggcaagc
     1801 acctcgatga cggcgtcgtc ttcctgatag ggatcgatcg agatctccag cacggtggac
     1861 ccgtacttca gatagaagtc gcaatgctcg gcgtcgtcga agtgctcatc gacgagctcg
     1921 tcgagatacg acttcacctg ccggtaaacc tcttcctggc aggcggtgtt gaattccttt
     1981 tcgcgcttgg cgggcatctc gggttgattg taaagcaaga accggcccgc ggaccggaat
     2041 gccggcgctc tttcgcgagc gccggcattc atcaggagcg attagtgtgc gggatgatgc
     2101 cgcggcgtaa cgaccagcgc gttcaccgcg acggagtcga tcgagccgca tgaattcgag
     2161 acgcgcacgg accacgtggc gtttccttcc gggttggtcg tgtacgacgg agagtcggtt
     2221 ccgacggcgt tcgtcacccc gctggcgtga tggacgaacc attgatagtg caggttcgcg
     2281 ccattcgcga cgacggtgaa cgtggcgttg ttccccggat tcacgatctg gccctgcggt
     2341 tgggtcgcga tcgtcggcgg cgcggtgacg ccgatgaacg tcgtcgcgcc cgggctgctg
     2401 ttggtgcaac tgctgtcagt cacattgcca accgttagcg tcgtcgacgt cgtgacgctg
     2461 atggtgcgcg atgcgggcga tgcgatgacc gtctgcgtga ggccgtcgga ccattgcacc
     2521 gtccacgggc cgttaccggt gagcgtgaac ggaatcgtga ccgtcgagga gccgcaggca
     2581 gagaggttcg ccgggacgct gatcgtgggc cccgaggtga ttgcgacatc cgcggtcgag
     2641 gaggcgctgc aagtgctcgc cgtggtcacg gtgaccgcaa ggtgcacgtt gccgctgttc
     2701 ccggcggtgt agaggatgtt gcgcgttccg atgccgcccg tgatcgtgcc gttcgtgatc
     2761 gtccagttgt agcttgctcc cgcgccggcg tcggccacgc tggcgtggtt gagcgtcgaa
     2821 agcgggcagg cagtgccgcc gttggtaatc gtggcggagg gaagcggatt gaagacgacg
     2881 gtggtcgata ccgcaatgct gccgccgcag gcgccggacg tggtcacggt caccgtgtag
     2941 gtgcccggct gcgtgactcc tgtcggattc tgcagtgtcg aggtgaagcc gcccggaccg
     3001 ctccacgagt agctcgcgcc gctgccggag acgaagaggt tgactgtgcc gcctgcgcac
     3061 ccgggaccgc tgttcgacgc agtcggcgcc gcgaccggcg ggcaagaacc cgtcgtcgat
     3121 gcgctgccgg tgatcgatgc agtcaccgta tcggtcgcgg tgaccgtctg cgcgccgctg
     3181 gcgttgagcg tgacgctgaa cgtatgcacg ccgttgtcgc cggcgacgaa cgtgtagtcc
     3241 gagggaaggg tcgaagaacc tgccgaggtg gtgaagtgag ccgtgcccgc gtaggtagtc
     3301 acggtcgcgt tcgaggcatc gagcgcggtg acagtcacgt tgaacggtgt gccgggagca
     3361 aaggtgccgg gagcggtgac gctgaagtgc gtggttgccg cgagcacgtt cgtgtttgcg
     3421 gtgccggtga tcgaggcggt aaccgtgtcc gttgccgtga tcgtccgcga tccgttcgat
     3481 gtgagcgtca cgctgaacgt gtgcgcgccg aggtcgccgg cggtgaacgt gtaatccgaa
     3541 ggcagcgtgc cggatgagga gctggtgaag tgcacggtgc cggtatagcc ggtagccgcg
     3601 gtatttgagc cactcagtgc gctgacggtg acgttaaagg caacgccgtt ggtcacgttc
     3661 gccggtgcag tgacgctgaa gtgggtggcg ggcggcggcg gattgaccgt cacgttgacg
     3721 gtggcagttg cgcagagcga atctgccgat ccggcggtcg tgccgttgtc gcagacggtg
     3781 tacgtgaagc tggccggacc gctgtagccg gctgccgggg tgtacgtgat ctggccgctg
     3841 gtaagcgaaa ccgtaccgtg ggtgctgccg gtcggcgtga ctgatgtgac ggtgagcgtc
     3901 tggccggctt cgttggccgg tcccgcggag tcgttggccg tcagatcgct gctggcgaag
     3961 gtcagcgcga cgttcgccgt ggcagtcttg ctgtcatcca ctgctgacgg tggatcgttc
     4021 acctcggcca ccgtgatggt catggttgcg gtgttggagt tgttcgtgcc gtcgttgacg
     4081 cgccacgtga acgtatcggt gccgttgaaa tcgggagccg gagtgtaggt ttgattcgcg
     4141 cccgttccgg tcagaccgcc gtggaaggga ccggtgagga tcgtgtaggt gagcgcgccg
     4201 cccggggtcg ccgcgtcgag cgtgatcgat ttctgcacgt cttcgttggt gctcgtcgat
     4261 tggtcggcgg cgcccggcgg gtttgtgccg tcgactccgg cctcgatggc gttctgaatc
     4321 gctggtggaa cgttggagaa gaggtcgtat ccggtgagcg cttccacctg gtcgacgctc
     4381 ttcagataga tgtgccagtc gtcattccgg atgccctgga tattgggcat gatcacgcag
     4441 atcgtccgcg tcgaggcagt gacgcgtgcg acgtcgtcgc cggatgcttt tggtaaaacc
     4501 agcgcgcact tccaagtgct cgccggaacg gtaacgtggc cgttcgcgac ggtgttggtg
     4561 acgccgccgt tgctgcccgt tccgcctgta cccgctggtc cggacacgat atacagctca
     4621 tcgacagaaa ggagagaacg cagatagttc tcgaggttcg cccacggtcc ctggttgttg
     4681 tcaggcgcct gcgcgaccat gttcgacatc aggaacgtag cttggttgat ggggatcgat
     4741 gtctccttgt cgcggtcggc gttcggcgtc atatgcccgc ggtcaaagcc ggacgcgaag
     4801 aagtcgctgg ccagcacgcg ataccaggtg ggcggtactt ccggatcggg ccggaacgtg
     4861 tcgacacgag tcaacgagcc aacccactca tcgctgagat gccagctgac ccagttcgga
     4921 cggccgaggt cacggttgta gcagatcgaa ttctcgggct tcagcatcag gtaggcgttt
     4981 ggagatgaca ggctggtcgt acaaatcgat ggctggccca tcgtcagatg aacggatggt
     5041 ggatacggag gcagggttcc cgaggagacg gtgaacgtcc acgagtagtc ggcgggcagc
     5101 gtgtcggtgc gcggcgcgct gtcgtccgtg tcgcggtcgt gcacggagtt ctggaggatc
     5161 gtcaccgtgc actgctccgc ggcgacgaag ttggcgttgg gcgtgatcac ccatgtgttc
     5221 ggagcgcctt cgacg
//
LOCUS       DATLVW010000572         6906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_394755, whole genome shotgun sequence.
ACCESSION   DATLVW010000572 DATLVW010000000
VERSION     DATLVW010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394755"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VNN25_16915"
     CDS             complement(<1..1299)
                     /locus_tag="VNN25_16915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HXH93267.1"
                     /translation="MTDPKTLNLADFRKRIGSEYWRSLNELSRDDFEQVVETEFPQGA
                     LALNRGVDRRDFVKLMGASMALGGLAACNRPAEKIVPYVRQPEGLIPGKPIFFASAMT
                     ISGYAHGVLVESHMGRPTKIEGNPDHPSSLGATNSFMQASILGLYDPDRSQVVRHLGE
                     ISTYSDFLAALQPELKAGTANGTGLRILTQTVTSPTVGAQIQQVLAQFPGAKWHQWEA
                     AGGHNVREGLRLAFGSYANAVYHFDKANVVVALDSDFLERGPGHLRYARDFSGRRRVR
                     KGTKSISRLYAIESGPTGTGSIADHRLAVKPSEVETYARAIAASVQGSDTRLPTPDPQ
                     FIAALVRDLQKNRGTSIVVAGEEQPPAVHAVAMAINQALGNIGATITITDPVEVAPVN
                     QLDSLKQLVADMNAGAVKTLIIIGGNPAYDAPGDLPFVKAM"
     gene            complement(1394..2041)
                     /locus_tag="VNN25_16920"
     CDS             complement(1394..2041)
                     /locus_tag="VNN25_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXH93268.1"
                     /translation="MAQIFHRSTNTLARVTIFGAVFAVAFVLWAIGGVVRSPYATDQG
                     IEREQPVPFSHQHHVAGLGIDCRYCHTSVETSNFAGLPPTQTCMNCHSQIWTNAEMLE
                     PVRASFANNKPIVWSRVHRLPDFVHFNHSIHVNKGIGCASCHGRIDKMALTFQASPLT
                     MEWCLQCHRAPEQFVRPRNEVFNMAYITTDQETIGPKLVREYNIQKLTTCSTCHY"
     gene            complement(2289..3668)
                     /locus_tag="VNN25_16925"
     CDS             complement(2289..3668)
                     /locus_tag="VNN25_16925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH93269.1"
                     /translation="MAKAPFILLVEDHQKILVNTMLQLREEGFAVHAVDNIVAAQEFL
                     VDQQPDLLILDVRLGADSGIDLIQRLIAQKRFPPTIIISGEASITETVEALRLGVYDF
                     IEKPFTRERLLQSVRNCLDHASLRRELTELRSRANVAKTIIGRSTEMIQLRDRIEKVA
                     ATDARVLIRGESGTGKELVANMLHRHSARRQKPFVKTNCAAIPAHLIEDELFGHARGA
                     FTDAKNAKAGLFEEADGGTLLLDEIGDMDVALQSRLLRVLEDGKVRRIGETQERQVNV
                     RVVAASNRDLQQLIGQRLFREDLYFRIATVPIDVPPLRQRVADIPLLFTHFLEVFCAE
                     NQRRRLTVDPEVIPAIEGYRWPGNVRELRNVCEQLSIFGSDPITIDQLPTSILQAEPV
                     GESGVLRLTETAVDLSLRGFRELCEREYIERVLRRVNWSFPAAAKILDIQRTYLHQKV
                     TTLKIARRK"
     gene            complement(3661..4866)
                     /locus_tag="VNN25_16930"
     CDS             complement(3661..4866)
                     /locus_tag="VNN25_16930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93270.1"
                     /translation="MKTRTISLILVVVVTLTGFAWFFRQLSFVSVGLAANSEVQRALR
                     QSLSDQKSLARSDPSHAALYRQRFEDVRLLLTRMEILSLTGRKLTSALEGTLLALVAL
                     VIASGSIIYLIEHRRRERRLVQLRESLMALSRGEPVASPLRKRRDLIGRIGLMIESTS
                     NAIDRDRRRMRSLEHLSSWQEAARRHAHEIRTPLTAARMEVARLLSWAGQQLPGEQAA
                     LASAQASIFEELERLRAFTTAFTSFAMLSPPRLVPLDLARFLEDFAGTFASAWPGLRL
                     ESQHGGERCEVMMDANLMRQLLVNLANNSAQACDGRTGLLRLRAWREGKQVLADVADD
                     GPGISASVRERLFEPYTTTRQIGEGMGLGLSICKKIMLDHGGDLDLVSSERGATFRLT
                     FPAGDELRG"
     gene            complement(4863..5474)
                     /locus_tag="VNN25_16935"
     CDS             complement(4863..5474)
                     /locus_tag="VNN25_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93271.1"
                     /translation="MLALLGAAAPRGDVPHVFARPDGAILIRLPTSSLTGDRVKTQLR
                     SGLTTAFVLLTKGRDSENHERKLGARVEIRYELWDENYIVVVIDGTGLRESRTIDSYA
                     HLVDWWSRSPILLGAPSPLRQSGAVAVSLEMVPFSPEEEADTQRWLAHSLNTSSSRAA
                     SGQGAASPQPSEGLLDIIIGTAIHRQAILSFHWDVRVEASPPK"
     gene            complement(5501..6730)
                     /locus_tag="VNN25_16940"
     CDS             complement(5501..6730)
                     /locus_tag="VNN25_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93272.1"
                     /translation="MLLLLAVAAPAAAEVARFPIETISIRGTHFASVSVMLAESHLVA
                     GHSYTESELHDGVLRINRLPFVLHTDVRLEKGSARGAYVLVITVEETKPLFISLGEIR
                     RELSAEIPVFDSRAELVGFRNEIVRSTADRGTLGGRIFVGSKGLLTASITRQGVDHYS
                     LGYTQYNLFGTNASVAVLADYVLRNYSIPVQFGPSPRVTAADQVKAQLIVAVPLFGNN
                     ALRGGIDHAPEVFHFGGAPGVPLRSTIVGNDRLQLLWVYDSTTDPIFPTNGTYADAGL
                     DVVSAPLSIKSGNSAQFNDYRVAHQKDFEARVDRYYEFVPGQSVQAGGDVLSHDGGTY
                     REYIVRGGYSVDMLPRWRELNGADLRFEVHLSRDYLAFPNQTTTSRGIADAGFAFRNS
                     WGLLRFDFEYTGWRRPK"
BASE COUNT         1357 a         2066 c         2189 g         1294 t
ORIGIN      
        1 catcgccttg acgaatggaa gatcgcccgg cgcgtcgtag gcgggattac cgccgatgat
       61 gatgagcgtc ttgaccgcgc cggcgttcat gtcggcgacg agttgtttga gcgaatcgag
      121 ttgattcacc ggcgccactt cgacgggatc ggtgatcgtg atggtcgcgc cgatgttgcc
      181 gagagcctgg ttaatggcca tggcaacggc gtgaacggcc gggggttgtt cttcgccggc
      241 aacgacgatg ctggtgccgc ggtttttctg cagatcgcgg acaagagcgg cgatgaattg
      301 ggggtcgggg gtcgggagtc gggtgtcgga gccttgaacc gatgcagcga tggctcgtgc
      361 atacgtctca acctccgatg gcttcacagc cagccggtga tcggcgatcg atcccgtacc
      421 ggtcggaccc gattcaatcg cataaagacg actgatcgat ttcgttccct tgcgcactct
      481 gcggcggccg gagaaatcgc gggcgtagcg aaggtgaccc ggtccgcgct cgaggaaatc
      541 agagtcgagc gcgacgacga cgtttgcttt gtcgaagtgg tagacggcgt tggcgtagct
      601 gccgaacgcg agacgcagcc cttcgcgcac gttgtgtccg ccggcggctt cccactgatg
      661 ccatttcgcg ccgggaaact gcgcgaggac ctgctggatc tgcgcgccaa ccgttggcga
      721 ggtgacggtc tgcgtgagga ttcgaagccc ggtgccgttc gccgtgcctg ctttcagctc
      781 gggctgcagc gcggccagga aatcgctgta cgtcgagatc tcgccgaggt ggcgcacgac
      841 ctgggaacgg tcgggatcgt agaggccgag gatggaggcc tgcatgaacg aattcgtcgc
      901 gccgaggctg gagggatggt caggattgcc ctcgatcttc gtgggacgtc ccatgtgact
      961 ctcgacgagc acgccgtgcg cgtagccgct gatcgtcata gccgaggcga agaaaatcgg
     1021 cttgcccggg atgagccctt ccggctggcg gacgtacggg acgatcttct ccgcgggacg
     1081 gttgcaggcg gccagaccgc cgagcgccat cgacgccccc atcaacttca cgaagtcgcg
     1141 ccggtcgacc ccgcggttca gcgccagcgc accctgcgga aactccgtct cgacgacctg
     1201 ttcgaagtcg tcgcgcgaga gctcattcag gctgcgccag tactcgctgc cgatgcgctt
     1261 gcggaaatcc gcgaggttca gtgttttggg gtcggtcatg atgtttcgcg ggagtcggtt
     1321 gtcgggggtc gggtgtcgga gcaagaagac gtcgcgcttc ttctccgact cccgactccc
     1381 ggcaaacgac cccctagtaa tgacatgtcg aacacgtagt cagcttctga atgttgtact
     1441 cccggaccag tttcgggccg atggtttcct ggtccgtggt gatgtaagcc atgttgaata
     1501 cctcgttgcg cggacggacg aattgttcgg gcgcgcggtg gcactgcagg caccactcca
     1561 tcgtcagcgg cgaggcctgg aacgtgaggg ccatcttgtc gatgcggccg tggcagctcg
     1621 cgcagccgat ccctttgttg acatggatgg agtgattgaa atggacgaaa tcggggagac
     1681 ggtggacgcg cgaccagacg atcggcttgt tgttggcgaa gctggcgcgc accggctcga
     1741 gcatctcggc gttcgtccag atctgtgagt ggcagttcat gcaggtttgc gtcggcggga
     1801 ggccggcgaa gttcgaagtc tcgaccgagg tgtggcagta gcggcaatcg atgccgagtc
     1861 ccgctacgtg atgctgatgg ctgaatggca ccggctgctc gcgctcgatt ccctgatccg
     1921 tggcgtatgg cgaccgcacc acgccgccga tcgcccacag cacgaacgcc acggcaaata
     1981 cagcgccgaa gatcgtgacg cgggcgagag tattggtgct gcggtggaaa atctgagcca
     2041 tttacaggct gctaccgtcc gggattcggg agtcgtgaaa cagaaaggcg gggagcaagc
     2101 gccacggcgg atggccgcgc ttcctcgcga aacggcggcg atcatatcca aaaattgcgg
     2161 tgacgcaaga cacggtattc agtgcggtcc gcgcgtcctg ccgcgcgccg cggatgccga
     2221 gtgcagaaag cgtgcgcggt tgggctcgtc tgccgcgtgg tccttgaatc cggccccggg
     2281 caaatcgctc atttccgccg cgcgatcttg agcgtcgtca ctttctgatg aaggtaggtt
     2341 cgctggatat caagaatctt cgccgccgcg ggaaagctcc aattcacgcg ccggagaacc
     2401 cgttcgatgt actccctctc gcacagttcc cggaacccgc ggagcgagag atcgaccgcg
     2461 gtttccgtaa gccgaaggac gccgctttcc cctaccggct ccgcctgcag gatcgacgtc
     2521 ggcagctgat cgatcgtgat gggatccgat ccgaagatcg agagctgctc gcaaacgttg
     2581 cgcagctccc ggacgtttcc cggccatcgg tagccttcga ttgccggaat cacctcagga
     2641 tcgacggtca gcctacgacg ttgattttca gcgcagaaca cctcgaggaa gtgtgtaaag
     2701 aggagcggga tgtccgccac gcgctggcgc agcggcggaa cgtcgatcgg aaccgtggcg
     2761 atgcggaagt agaggtcttc gcgaaacaag cgctgtccga tcagctgctg cagatcgcgg
     2821 ttgctggccg cgaccacgcg cacgttgacc tgccgttcct gcgtttcgcc gatgcgccgg
     2881 accttgccat cctccaggac ccgcaggagc cgggactgga gggcgacatc catatcgccg
     2941 atctcgtcga gcagcagcgt cccgccgtct gcttcctcga agagtcctgc cttggcattc
     3001 ttcgcatcgg tgaaggcgcc acgcgcgtgg ccgaacagct catcctcgat gagatgcgcg
     3061 ggaatggcag cgcagttggt cttgacgaac ggcttctgtc ttcgcgcgct gtggcggtgg
     3121 agcatgttgg caaccaactc tttacctgtg ccgctctcgc cgcggatgag gacgcgtgca
     3181 tcggtcgctg ccaccttctc gatgcggtcc cgtagttgaa tcatctccgt cgatcggccg
     3241 atgatcgtct tcgcgacatt ggcgcgggat cgaagttcgg tgagctcgcg ccgcagcgac
     3301 gcatggtcga gacaattgcg aacggactgg agcagacgct cgcgcgtgaa cggcttctcg
     3361 ataaagtcgt agacgccaag ccgcagagct tccacggtct ccgtgatcga ggcctcaccc
     3421 gaaatgatga tcgtcggcgg gaaacgcttc tgcgcgatga ggcgttggat gaggtcgatg
     3481 ccgctgtcgg cgccgaggcg gacgtcgaga atgagaaggt cgggctgttg atcgacgaga
     3541 aactcctgcg cggccacgat gttgtcgaca gcgtgcacgg cgaagccctc ttcgcgtagt
     3601 tgcagcatcg tgttgaccag aatcttctgg tggtcttcca cgagaagaat gaagggcgcc
     3661 ttagccacga agttcatccc ctgccgggaa ggtgagccgg aaagttgctc cccgctcgct
     3721 ggaaacgaga tcgaggtcgc cgccgtggtc gagcatgatc ttcttgcaga tggaaagccc
     3781 gaggcccatc ccttctccga tctgcctcgt agtcgtgtaa ggctcgaata aacgctcgcg
     3841 aacagaggcg gaaatgcccg ggccgtcatc cgcgacatcc gcgagaacct gtttcccctc
     3901 ccgccaggcg cgaagacgca gaagtcctgt ccggccatcg caggcctggg cactgttgtt
     3961 tgccagattc acaagcagtt gccgcatcag gttcgcgtcc atcatcacct cgcatcgctc
     4021 tcctccgtgc tgggattcca gccggagacc gggccaggcg ctggcgaagg taccagcgaa
     4081 atcctccagg aagcgggcca gatcgagcgg cacgagccgc ggagggctga gcatcgcgaa
     4141 ggatgtgaat gccgtggtga aggcccgcaa ccgctccaac tcctcaaaga tactggcttg
     4201 cgcactcgcc agcgctgcct gctcgccggg aagctgctgc ccggcccagg aaagaaggcg
     4261 cgcaacctcc atcctcgcag ccgtaagcgg tgtacggatc tcgtgcgcat gacggcgtgc
     4321 cgcttcctgc caggaggaga ggtgctcgag agatcgcatc cgccgccggt cacgatcgat
     4381 ggcgttcgac gtcgactcga tcatgagacc gatccgtccg atcaggtccc ggcgtttcct
     4441 gagcggtgac gctacaggct caccgcgcga caaggccatg agcgattcgc gaagctgaac
     4501 gagtctccgc tcgcgccttc gatgctcgat caggtaaatg atgcttccgg aagcaatgac
     4561 gagggcaaca agagcgagga gggtaccttc gagtgcactc gtcagcttgc ggccggtcag
     4621 cgaaaggatc tccattcgcg tcagcagcag ccgcacatcc tcgaagcgct ggcgatacaa
     4681 agcggcatgg ctgggatcgg agcgcgccag acttttctga tcgctcagac tctggcgcaa
     4741 tgcacgctgg acttcgctat tcgccgcgag cccgaccgaa acgaacgaga gctggcgaaa
     4801 gaaccaggcg aatcccgtca acgtgacgac gaccaccagg ataagagaga tcgttcgtgt
     4861 tttcattttg gcggcgacgc ttcgacgcgc acatcccagt gaaacgagag gatggcctga
     4921 cgatgaattg ccgtgccgat gatgatatcg agcaagcctt ccgacggctg cggacttgcg
     4981 gccccttgcc cactcgctgc acggctgctc gaggtgttca gcgaatgcgc gagccatcgc
     5041 tgcgtgtcgg cctcctcctc cggcgagaag ggaaccattt cgaggctgac cgcgaccgcg
     5101 ccggactgac gcaacggcga aggcgcgcca aggaggatcg gcgagcgaga ccaccagtcg
     5161 accaggtgcg catagctgtc gatcgtccgg ctttcccgca gccccgtacc gtcaatcacg
     5221 accacgatgt agttctcatc ccacagctcg taacgaatct caacacgagc accaagcttg
     5281 cgctcgtggt tttcggaatc cctccccttc gtgagcaata caaaggctgt cgtgaggccg
     5341 ctacgcagct gcgttttgac tcgatcgccg gtgagcgagc tcgtcggaag ccggatcagg
     5401 atcgcgccat ccgggcgggc aaagacgtga ggaacgtcgc cccgaggcgc agcagcgccc
     5461 agcagagcga gcaaacaggc gattggcgcg cttctgaacc tcacttgggc cggcgccatc
     5521 ccgtgtattc gaaatcgaat cggagcagtc cccacgaatt gcgaaacgcg aagccggcat
     5581 cggcaatccc gcgcgaggtc gtggtttgat tcggaaatgc gaggtaatcg cgggaaaggt
     5641 gcacctcgaa tcgaaggtcg gcaccattaa gttcccgcca tcgtggcagc atgtcgaccg
     5701 aatagccgcc gcggacgatg tattcgcggt acgtgccgcc atcgtgagac agcacgtcgc
     5761 cgccagcctg aacggactgg ccaggcacaa attcgtaata ccggtcaacg cgagcttcga
     5821 agtccttttg atgagcaaca cgatagtcat tgaattgagc cgaattcccg ctcttgatgc
     5881 tgagaggtgc cgatacgacg tccagcccgg cgtcggcgta cgttccgttc gtggggaaaa
     5941 tcggatccgt cgtcgagtca tagacccaaa gcaattgcaa ccggtcattc cctacgattg
     6001 tcgaccgcaa cggcacgcct ggtgccccac cgaagtggaa gacctcgggt gcgtgatcga
     6061 taccgccgcg caatgcattg ttcccaaaaa gcgggaccgc cacgatcaat tgtgctttca
     6121 cttgatcggc cgccgttaca cgcggtgatg gtccgaactg taccggtatg gagtagttcc
     6181 gaagtacata gtcggcgaga actgccacgg acgcattcgt cccaaagagg ttgtattgcg
     6241 tgtagccgag agaatagtga tcgactccct ggcgcgtgat tgacgcggtc agcaaaccct
     6301 tgctgcctac gaaaatcctg cctcccagtg tcccgcggtc ggctgttgag cgaacgatct
     6361 cgtttcggaa ccctaccagt tcggcacgtg aatcgaacac tggaatctca gcggaaagtt
     6421 cgcggcgtat ctcgccaagg ctgatgaata gtggcttggt ttcctcaact gtaatcacta
     6481 gaacatacgc accgcgtgcc gatccctttt cgagacgcac gtcagtgtga agcacaaacg
     6541 gcagacggtt gatccgaaga acgccgtcgt ggagctccga ttccgtgtaa ctgtgaccgg
     6601 caaccagatg cgactctgcc agcatcacgg agaccgacgc gaaatgcgtc cctcgaatcg
     6661 agatcgtctc gatgggaaat cgcgcaacct cggctgctgc cggtgctgcg accgcgagaa
     6721 gcaacagcat tagtcgtccg aactgcgcac gaagaaagcc ccgcgccgga gtacgaaacc
     6781 gggccggaga cagcaccggc ctgtcaaacg ggaaccatgc attcgtcaat gtgccacacc
     6841 cctggaccgg ccagccggct cgaccgtaaa cgcaggcgat agcgtgtacg tgagcgtgtt
     6901 accggg
//
LOCUS       DATLVW010000573         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_435429, whole genome shotgun sequence.
ACCESSION   DATLVW010000573 DATLVW010000000
VERSION     DATLVW010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435429"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3151)
                     /locus_tag="VNN25_16945"
     CDS             complement(<1..3151)
                     /locus_tag="VNN25_16945"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93273.1"
                     /translation="MKRITIVLAAVLTIALAVISIRAQSSPELPALMRQTQAEADAKS
                     AGCLSCHQGIEPMHASAAVKLACTDCHGGDGNARTKEAAHVKPLHPEVWKTSANPQRS
                     YTALLKEPVEFVKFINPGDLRVSQETCGACHQRQVNAVPRSTMTTSAVFWAAVSYANG
                     LVSRKQGLLGESYSREGNAQSLKPATPPTPEQIAKGALPVLVPLPRWEIFQPAEYFRA
                     FERGGISGSSSFPDIGNPSPEVDAGRPDIRLGNRGRGTGLRISPALINLNKTRLNDPH
                     LSFMGTNDHPGDYRSSGCTGCHVVYANDRDPLHSAGYGQYGNTGLSITGDKSIPKNEQ
                     GHPLRHQFTRAVPTSQCMSCHMHQPNSFVNTYLGYTMWDYESDAELLWPKKQKYPTQA
                     ERYASLDANPEGAAPRGLWSDEKFLENVTDLNSQAKNTQFADYHGHGWNFMAVYKRDH
                     KGNLLDGDGKQVSFDDPAKFKKAVHLQDIHLEKGMHCADCHFSQDEHGNGQIYAEYGN
                     NIEIECQDCHGNVDAYTNLRTSGPAAPPGGTDLLLGTTPFGQRRFIWVNGKLYQRSML
                     NPGMQWEVIQVKDSITPGNAHYNERSAYAKTIQRDGSWGARAGANLAHADAKMTCYAC
                     HTSWMTSCSGCHLPQEQNAKTEVHHYEGGETRNYASYNPQVIRTDVFMLGVNGTTKGH
                     RLAPVRSSSALVISSTNALRDRIYIQQPPISSAGYSSQAFNAHVPHTVRSRETQGCGS
                     CHVTKENDNNAWLAQLLLQGTNFVNFIGRYAYVAEGKGGFEGVCVTEWDEPQAVIGSS
                     LHKIVYPDYYAKHQQNKMELQVAHEHHGDSRSLQMRGEFLYSANGEEGFSVYDVANID
                     NKDFSEHIVSAPVSPLGQRTFVKTKFASAVALPTTMPIDPNRQTQFIPENQEQPIHPL
                     YKYVYVADREEGLILIDVTTLADANPSNNFLTRAVTFNPGGALSGARSIYVAGRWVFI
                     GAAGGLVIVDIDKPLAPRIVATIPELKDATGIAVQFRYAFVTTHDGLSTVDITDPSHP
                     RVAGHVAIPDARSVYVARTY"
     gene            complement(3151..>5192)
                     /locus_tag="VNN25_16950"
     CDS             complement(3151..>5192)
                     /locus_tag="VNN25_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93274.1"
                     /translation="PVRLRGAIGRTRESDLRFASFFFVALALLAITAFAQPANTITIN
                     DDQPVYAEGVPFMLSGTSAPGAVIAVAIEKSGNGQTTADTTGHWTLMWTPPVKTGTWT
                     ITATSGGVTATQLLRVQLPGNVQRQPIVEPVLRYGTPEIPHPEAYQEMTDRWRIAPPP
                     YELTEKPKARAIGKNGATLDPYNQNLIKGDLPIFGKDWFFDFTGISDTLAESRTLPTP
                     SGVSAVKPTSITVFGKDNQNLFNENLIASFDLFQGDTAFKPIMQRFKATLIANLNYVH
                     LRENGLVKPDVRRGTSRTDGALSLQEFFYERKLKDVSPNYDFISIRVGSQPFNSDFRG
                     FIFNDTNLGVRVFGNFASNRYQYNVAVFDRLEKDTNSGLNTMALRDQQVAIANIYLQD
                     FLVHGYTQQFSVHYVRDEATFHYDRNSILVRPAPVGVFTPHEIRATYLGESGLGHIGR
                     FNVDHAVYYVFGHDDLNPIAGPDPKLKDGNGVQVGAGSAALEVSYDRDWIRPRLAFFY
                     ASGDRSPRDRKATGFDAIFDSPNFAGGGFSFFNRLGIRLAGTGVALVERGSLLTSLRS
                     SKDEGQPQYVNPGVQIASAGLDVDVTPRLKAIFTGNYIRFDAVQSVEAILFQGNIHKE
                     LGTDLSIGLRYRPLLNQNVVIVGGSALFLPGRGFKDIYERDRPLVHVFTNVILTF"
BASE COUNT          899 a         1484 c         1754 g         1055 t
ORIGIN      
        1 cgtacgtgcg cgcgacgtag acgctgcggg cgtcgggaat cgcgacatga ccagccacgc
       61 gcgggtgcga ggggtcggtg atatcgacgg tcgacaatcc gtcgtgcgtg gtcacgaagg
      121 cgtagcggaa ctgcaccgcg atgccggtag catctttcaa ttccggaatg gtagcgacga
      181 tccgtggcgc gagcggcttg tcgatgtcga cgatgaccag tccccccgcg gcaccgatga
      241 acacccatcg cccggccacg tagatcgacc gcgcgccgct cagggcgccg ccggggttga
      301 aggtcacggc gcgcgtcagg aagttgttcg acggattggc gtcggccagc gtcgtgacgt
      361 cgatgagaat gagtccctct tcacggtcgg cgacgtacac gtacttgtac agcgggtgga
      421 tcggctgctc ctgattctcc ggaatgaact gtgtctgccg gttcggatcg ataggcatcg
      481 tcgtcggcag cgccacggcg gacgcgaact tggtcttcac gaaggtacgc tgtccgagcg
      541 gagaaaccgg cgcggagacg atgtgctcgg agaagtcctt gttatcgatg ttggcgacgt
      601 cgtagacgct gaagccttct tcgccattcg cgctgtagag gaactctcct cgcatctgca
      661 gcgagcgtga gtcgccgtgg tgctcgtgcg cgacctggag ctccatcttg ttctgctgat
      721 gcttggcgta gtagtcggga tagacgatct tgtgaagcga cgatccgatc accgcctgcg
      781 gctcatccca ctcggtgacg cagactcctt cgaagccgcc cttcccttcc gcgacgtacg
      841 cgtagcggcc gatgaagttg acgaagttcg ttccctgcag caaaagctgg gcgagccagg
      901 cgttgttgtc gttctctttc gtgacgtggc agcttccgca accctgcgtc tcacgcgacc
      961 gaacggtgtg cggcacgtgc gcgttgaatg cctgactcga atatccagcc gaagagatcg
     1021 gcggctgctg gatgtagatg cgatctcgta gcgcattcgt cgagctgatg acgagcgctg
     1081 atgacgagcg caccggagcg aggcgatgtc ccttcgtcgt cccgttcacg ccaagcatga
     1141 agacgtcggt gcggatgacc tgcgggttgt atgacgcgta gttcctcgtt tcgccgcctt
     1201 cgtagtgatg cacctcggtc ttcgcgttct gttcctgcgg gagatggcag cccgagcacg
     1261 acgtcatcca cgatgtgtgg caggcgtagc acgtcatctt cgcgtcggcg tgcgcgaggt
     1321 tcgcgcccgc acgcgcgccc caggagccgt cgcgctggat cgtcttcgcg tacgccgagc
     1381 gctcgttgta gtgcgcattg cccggcgtga tcgagtcttt gacctggatc acttcccact
     1441 gcatgccggg attgagcatc gagcgctggt aaagcttgcc gttgacccag atgaagcggc
     1501 gctggccgaa aggcgtcgtt ccgagcagca gatcggtgcc gcccggaggc gcagccggac
     1561 ccgacgtgcg caggttcgtg tacgcatcga cgttgccgtg acagtcctgg cattcgatct
     1621 cgatgttgtt gccgtactcg gcgtagatct ggccgttgcc atgctcgtcc tgcgagaagt
     1681 ggcagtcggc gcagtgcatc cccttctcga ggtggatgtc ctgcagatgc acggccttct
     1741 tgaatttggc gggatcgtcg aacgaaacct gcttcccgtc gccatcgagc agattgcctt
     1801 tgtgatcgcg tttgtagacg gccatgaagt tccagccgtg accgtgatag tcggcgaact
     1861 gggtgttctt cgcctgcgag ttcagatcgg tgacgttttc aaggaacttc tcgtcggacc
     1921 acaatccgcg cggcgcggcg ccttccggat tggcgtcaag cgatgcatat cgctcggcct
     1981 gggtggggta cttctgcttc ttcggccaga gcaactcggc gtccgattcg tagtcccaca
     2041 tcgtgtagcc gaggtaggtg ttgacgaaag aattcggctg atgcatgtga cacgacatgc
     2101 attggctcgt cggcacggcg cgcgtgaact ggtggcggag cggatggccc tgttcgttct
     2161 tcggaatcga tttgtcgccg gtgatcgaga gcccggtgtt tccgtactga ccgtagccgg
     2221 cggagtgcaa cggatcgcga tcgttggcgt agacgacgtg gcatccggtg caacccgacg
     2281 aacggtaatc gccgggatga tcgttcgtgc ccatgaacga gagatgcgga tcgttgagac
     2341 gcgtcttgtt gagattgatg agcgcgggcg agatgcgcag tccggtgccg cggccgcggt
     2401 tgccgaggcg gatgtccggc cgccccgcat cgacctcggg actcggattg ccgatgtcgg
     2461 ggaacgaact ggagcctgag atgccgccgc gctcgaacgc gcggaagtac tcggccggct
     2521 gaaagatctc ccagcgcggc agcggaacga gaaccggcag cgcgcccttc gcgatctgct
     2581 cgggcgtcgg cggcgtggcc ggcttgagcg actgcgcgtt gccctcgcgg ctgtaactct
     2641 cgccgaggag gccttgcttg cggctgacga gaccgttggc gtagctgacc gcggcccaga
     2701 aaaccgcgga cgtcgtcatc gtgcttcgcg gcacggcatt gacctgacgc tgatggcagg
     2761 cgccgcacgt ttcctgtgac acgcgcaagt cgcccgggtt gatgaacttc acgaactcga
     2821 ccggctcctt caggagtgcc gtgtacgagc gctgcggatt cgccgacgtt ttccacacct
     2881 cgggatgcag cggcttgacg tgcgcggctt ccttcgttcg ggcgttgccg tcgccgccat
     2941 ggcaatcggt gcaggctagc ttcacagcag ccgacgcgtg catcggctcg atgccttgat
     3001 gacacgagag gcagcccgcg ctcttggcgt cagcctcggc ctgcgtctgt cgcatgagcg
     3061 cgggcagttc tggcgacgac tgcgcacgga tgctgatcac ggccagcgcg atggtgagaa
     3121 cagcggcgag tacgatcgtt atccgtttca tcagaaggtc agaatcacat tcgtgaagac
     3181 gtgaacgagg ggacggtctc tctcataaat gtctttgaat cctcggcccg gcaaaaacag
     3241 cgccgaacca ccgacgatga cgacgttctg attcagcagc gggcggtagc gaagtccgat
     3301 cgaaaggtcg gttccgagct ccttgtggat gttgccttga aagaggatgg cctcgaccga
     3361 ttgcaccgcg tcgaagcgga tgtagttgcc ggtgaagatc gctttgaggc gcggcgtgac
     3421 atcgacgtcg agtcccgcgc tggcgatctg aactccagga ttgacatact gcggctgtcc
     3481 ctcatctttc gagctgcgca gactggtcag cagcgagcca cgctccacga gcgcgacgcc
     3541 cgtgcccgcg agccggatgc cgaggcgatt gaagaaggag aagccgccgc cggcgaagtt
     3601 tgggctgtcg aagatcgcgt cgaagccggt cgccttgcgg tcgcgcgggc tgcggtcgcc
     3661 ggacgcgtag aagaacgcca gccgaggacg gatccagtcg cggtcgtagg agacttcgag
     3721 cgctgcgctg ccggcgccga cctgcacgcc gttgccatct ttgagtttcg gatcaggccc
     3781 ggcgatcggg ttgagatcgt cgtgaccgaa gacgtagtag accgcgtgat cgacgttgaa
     3841 acggccgatg tggccgagtc ctgactcgcc gaggtaggtg gcgcgaatct cgtgcggcgt
     3901 gaagacgccg accggggccg gacgcaccag gatgctgttg cggtcgtagt ggaacgtcgc
     3961 ctcatcgcgg acgtagtgga cgctgaactg ctgcgtgtag ccgtggacga ggaagtcctg
     4021 cagatagatg ttcgcgatcg cgacctgctg atcgcgaagc gccatcgtgt tgaggccgga
     4081 gttggtgtct ttctccagcc ggtcgaagac ggctacgttg tactggtagc gattcgacgc
     4141 gaaattgccg aagacgcgga cgccgaggtt ggtgtcgttg aagatgaagc cgcggaagtc
     4201 gctgttgaac ggctgcgaac cgacgcgaat cgagatgaag tcatagttcg gcgagacgtc
     4261 cttgagcttg cgctcgtaga agaattcctg gagtgagagt gcgccgtcgg tgcgcgaggt
     4321 tccgcgccgc acatcaggtt tgaccagccc gttctcgcga agatggacgt agttgaggtt
     4381 ggcgatcagc gtggccttga agcgctgcat gatcggcttg aacgccgtat cgccctggaa
     4441 gagatcgaac gaggcgatga ggttctcgtt gaagaggttc tggttgtctt tgccaaacac
     4501 ggtgatcgag gtcggcttaa cggcgctgac gccggacggc gtcggcaacg ttcgcgactc
     4561 cgcgagcgtg tcgctgattc cggtgaagtc gaaaaaccag tccttgccga agatcggcag
     4621 gtcacctttg atgaggttct ggttgtaggg atcgagtgtc gcgccgttct tgccaatggc
     4681 gcgggccttt ggcttttcgg tgagctcgta cggcggcggc gcgatcctcc agcgatccgt
     4741 catctcttga tacgcctcgg gatggggaat ctccggcgtg ccgtagcgca gcaccggctc
     4801 gacgatcggc tggcgctgca cgtttcccgg aagctgaacg cgtaagagtt gcgtcgcggt
     4861 gacgccaccg gacgttgcgg tgatcgtcca cgtaccagtc ttcaccggcg gcgtccacat
     4921 caatgtccag tgtccggtcg tgtcggcggt ggtttgtccg ttgccggatt tctcgattgc
     4981 gacggcgatg acggcgcccg gcgcggacgt tccgctcagc atgaacggca cgccctcggc
     5041 gtagacgggt tggtcgtcgt tgatggtgat ggtgtttgcg ggttgggcga aggcggttat
     5101 ggcgagtagc gccaaagcaa cgaagaaaaa cgaagcgaaa cgtaggtcgg actctctagt
     5161 ccgaccaatc gcgccacgga gtcggactgg ag
//
LOCUS       DATLVW010000574         4286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_150986, whole genome shotgun sequence.
ACCESSION   DATLVW010000574 DATLVW010000000
VERSION     DATLVW010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150986"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1202)
                     /locus_tag="VNN25_16955"
     CDS             complement(603..1202)
                     /locus_tag="VNN25_16955"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HXH93275.1"
                     /translation="MRCRILAATVLITFTAALAVAADDPKASAAKHQAEMEAMMKAAT
                     PGDAHKKLGAIVGTFNTDVKMWMEPGAPPTAGGGVSENSWALDGRFVQQQFTGTFMGM
                     PFHGIGYTGYDNIKKHYIGSWMDNFSTSMMVSSGTASADGKSITFTSTEDDPMTGKSS
                     EVKETLTVIDDDHHSLEMWGAGPDGKMFKMMEIAYSRKK"
     gene            1311..2759
                     /locus_tag="VNN25_16960"
     CDS             1311..2759
                     /locus_tag="VNN25_16960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXH93276.1"
                     /translation="MKVSFLKAPIGGIIGLEMITFVEPLGLECIAGGLEAEGHTCQIV
                     DMRIDGIEKGLAKMKAFAPEIVGLQCNFTTERFRALRLAKMVRTALPEALIVLGGHDA
                     SRDPNWFVKPVIDVVVVGDGEEVMTPLVAAWAKNKDLTKVAGLLINAPSGPIRTAAAP
                     ARPNIDELPFPARHLIKEYASEYYINFRRPLALLETARGCPFKCNFCSVWKFHESTFR
                     EKSPARVVKEIEQIEAPNIFITDDIFWMNVKRGRELGQALINSGIKKHYTIQTRSDII
                     CKFPKLVEQWKGCGRMTVFLGLEKVDDAGLKSVNKRNTADHNNQAIRILQELGVGYTP
                     NFIIDPTWEKSDFDRLKRWIDETGAYNSGFSVLTPLPGTDLWDEVEGQVNTRDWELYD
                     IAHTVLPTKLPLEEFYREYAGLWQHAMDVRYQIEGKLKTNLGLLAAIATRRVTLTAIR
                     KGMRMGSVLSDPASFMRAHDESAARLAEAATM"
     gene            2967..3560
                     /locus_tag="VNN25_16965"
     CDS             2967..3560
                     /locus_tag="VNN25_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93277.1"
                     /translation="MNEKRNAFRALVAASGFAVLLSSAAIAAPQPQERAYQYRADRIS
                     TQGEITSMAREGDQYRIELNHGSYVYYVPVSTIRNRDIRVGDRVRLGGVVSGDSVNAD
                     YIAFSGEPAYTSDPAYRGVPYGSSGWMSGTVISVNRRLNYVTIRDDASGLPVKVDVRN
                     MDTKRSVNVWRTRPGDHISVNGSWSNHDTFNSSIIQY"
     gene            complement(4064..>4286)
                     /locus_tag="VNN25_16970"
     CDS             complement(4064..>4286)
                     /locus_tag="VNN25_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93278.1"
                     /translation="ILARRYGCLIGAPMDHREMAAKDTAGQGRPAVAGKNACATLQPF
                     ARLTTNVTSSFDDAPGFTASRRRHFEKVK"
BASE COUNT          894 a         1326 c         1222 g          844 t
ORIGIN      
        1 tcgacggaat cccacctcat tcaaggccgg tcctctactt cccaccggtc tctggctcgg
       61 gaatcggacg ctatgactca ggcgtcgcct ctgcgtgacc ggaaagatag gtccgctgtt
      121 tccgaaagtc aacggtgcgg gcacataaaa cgcgtaaagt actgagcctg cgagcgatgt
      181 tccttcgtct ccgcaaagga ggcgtctcgc aggatactca gagtctcacg catgatactc
      241 acgggtatcg cgcatgagac tcagagtctc attcatgaaa ctcaggggta tcacgcataa
      301 gactcagagt ctctttcatg aaactcacgg gtatcacgca tgaaacccac gggtatcacg
      361 catgagactc acgggtatca cgcatgagac tcagagtctc attcatgaaa ctcgcgggta
      421 tcacgcatga aactcagagt ctcatgcatg agactcacgg atctcattca tgaaactcgc
      481 aagtctcatt catgagactc agggtctttc gcatgagact cgggggtctc attcatgaga
      541 ctcaagggtc tttcgcacgg acctctccca cccccacacc caacccccca ccccccacac
      601 ccctacttct tccgcgagta agcaatctcc atcatcttga acatcttgcc gtccgggccg
      661 gcgccccaca tctcgagcga gtggtgatcg tcgtcgatga cagtgagcgt ctctttgacc
      721 tcggacgact tgcccgtcat cgggtcgtcc tccgtggacg tgaaggtgat cgacttgccg
      781 tcagcggacg ctgtgccgct ggacaccatc atcgacgtgg agaagttgtc catccacgag
      841 ccgatgtagt gcttcttgat gttgtcgtag cccgtgtagc cgatgccgtg gaaaggcatc
      901 cccatgaacg tgccggtaaa ttgctgctgg acgaaacggc cgtcgagcgc ccaactgttc
      961 tccgatacgc cccctccggc ggtgggggga gcaccgggct ccatccacat cttcacgtcg
     1021 gtattgaacg tgcccactat cgcgccgagc tttttgtgcg catcgcccgg cgtggcggct
     1081 ttcatcatcg cttccatctc cgcctgatgt ttggcggcgc ttgccttcgg gtcgtcggcg
     1141 gcaacggcga gcgcggcggt gaacgtgatc aggacggtgg cggccaggat tctgcagcgc
     1201 atggttcttt ctcctcttgg tgaaacggtt gaccggggtg gagcgaaatt gtaaccgagc
     1261 cagcgtgtta cattgactcc tcgcgtacaa tttttgggaa ggtaatacag atgaaggtct
     1321 catttctgaa agcacccatc ggcggaatca tcggcctcga gatgatcacg ttcgtcgagc
     1381 cgctcgggct cgaatgcatc gccggcggcc tggaagccga ggggcacacg tgccagatcg
     1441 tcgacatgag gatcgacgga atcgagaaag gcctcgccaa gatgaaggcc tttgctcccg
     1501 agatcgtcgg gttgcagtgc aacttcacca cggagcgctt ccgcgcgctg cgtctggcga
     1561 aaatggtccg gaccgcgcta cccgaggcgc tcatcgtcct cggcgggcac gacgcgtcgc
     1621 gcgatccgaa ctggttcgtg aaacccgtga tcgatgtcgt cgtcgtcggc gacggcgagg
     1681 aagtgatgac gccgctcgtc gccgcctggg cgaagaacaa ggatctgacg aaggttgccg
     1741 gcctcctcat caacgccccc tccggcccga tccgtacggc tgcggcgccg gcacggccga
     1801 acatcgacga gctgccgttc cccgcccgcc atctcatcaa agagtacgcc tcggagtact
     1861 acatcaactt ccgccgcccg ctcgctctgc tcgagaccgc gcgcggctgt ccgttcaagt
     1921 gcaacttctg ctcggtctgg aagtttcacg agagcacgtt ccgcgagaag tcgccggcgc
     1981 gcgtcgtcaa agagatcgag cagatcgaag cgccgaacat cttcatcacc gacgacatct
     2041 tctggatgaa cgtgaagcgc ggccgcgagc tcggccaggc gctgatcaac tccggcatca
     2101 agaagcacta cacgatccag acccgcagcg acatcatctg caagttcccc aagctcgtcg
     2161 agcaatggaa gggctgcggc cgtatgaccg tcttcctcgg cctcgaaaaa gtcgacgacg
     2221 ccggcctcaa gtcggtaaac aagagaaaca cggccgacca caacaaccag gccatcagga
     2281 tcctgcagga gctcggcgtc ggctacacgc cgaatttcat catcgacccg acgtgggaga
     2341 aatccgactt cgaccgcctg aagcgctgga tcgacgagac cggcgcctac aactccggct
     2401 tctccgtcct caccccgctt cccggcacgg acctctggga cgaagtcgaa ggccaggtga
     2461 acacgcgcga ttgggagcta tacgacatcg cccacaccgt cctgccgacg aaactccccc
     2521 tcgaagagtt ctaccgcgaa tacgccggcc tctggcaaca cgccatggac gtccgctacc
     2581 agatcgaagg caaactcaaa acgaacctcg gcctgctagc cgcgatcgcc acgcgccgcg
     2641 taacgctcac cgccatccgc aagggaatgc ggatgggctc cgtgctcagc gatcccgcgt
     2701 cattcatgcg cgcgcacgat gagagcgcag cgagactggc cgaagcggcg acgatgtaga
     2761 acggcaaacc ctaaccacag agacacagag gtcacagagg aatgcttcaa ctctgtgtcc
     2821 tctgtgtcct ctgtgtctct gtggttcgtt ttgtcgtccc tttgcgacct ccagaacttc
     2881 atcgatcaga acgctttgag tctcgatcat ggcaacatct ttgcgatggt tatttggcga
     2941 agtgagacca tcctaaggag gattcgatga acgagaaacg aaatgctttc agagcgctcg
     3001 tcgcggcgag cggctttgcg gtgctgctca gttcggcggc gatcgcggcg cctcaaccgc
     3061 aggagcgcgc ttatcagtat cgcgccgacc gtatctccac gcagggggag atcaccagca
     3121 tggcccgcga gggcgatcag tatcgaattg agctcaatca tggcagctac gtctactacg
     3181 ttcccgtgtc cacgattcgc aaccgcgaca tccgcgttgg cgatcgggtc cgtcttggcg
     3241 gcgtcgtctc cggcgattcc gtgaatgccg attacatcgc gttttcgggt gagcctgctt
     3301 acaccagcga tccggcctac cgcggcgtgc cgtatggctc gagtggatgg atgagcggta
     3361 cggtcatcag cgtcaaccgc cggctcaact atgtaaccat ccgcgatgac gcctccggct
     3421 tgccggtgaa ggtcgacgtt cgcaacatgg atacgaagcg ctcggtcaac gtctggcgca
     3481 cacgcccggg cgatcacatc agcgtgaatg gctcgtggtc gaaccacgac acgttcaaca
     3541 gttcgatcat tcagtactaa gcgcaaccac cacgtgccgc cgggttgagc ccggcggcac
     3601 tgttgttggg cgatgagctt gaagtcacgg attgtgactt taagtctgcg gttgaccggc
     3661 gactctgcgg cccaccgcct tattcgcttt gtgcggtctc cacaacttga ggtcacaatt
     3721 tgttacctca aaggagtgaa agcttcgatt ggtcagggga aactcgcacc agcctatttc
     3781 gcaacggtcc gttggagcgc caatcaagaa accgtaccgc cgggcaagaa tgcccgacgg
     3841 tagcaggcaa gaatgcctgc actacttgac cttctcaaag tgcaggtgcc cgcgaagcag
     3901 tgaaaggctg aagagtagcg caggcattct tgcctgctac cgctgggcgt ccctgcccag
     3961 cggtgtcttt tgcggccatt tcgcgacggt ccgttggagc gccaatcaag aaaccgtacc
     4021 gccgggcaag aacgcccgac gggagcaggc aagaatgcct gcactacttg accttctcaa
     4081 agtgacggcg ccgcgaagca gtgaaaccgg gagcgtcgtc gaatgacgat gtgacgtttg
     4141 tcgtgaggcg agcgaacggc tgaagagtag cgcaggcatt cttgcctgct accgctgggc
     4201 gtccctgccc agcggtgtct tttgcggcca tttcgcgatg gtccattgga gcgccaatca
     4261 agcaaccgta ccgccgggca agaatg
//
LOCUS       DATLVW010000575         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_478001, whole genome shotgun sequence.
ACCESSION   DATLVW010000575 DATLVW010000000
VERSION     DATLVW010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478001"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /gene="sufD"
                     /locus_tag="VNN25_16975"
     CDS             <1..1174
                     /gene="sufD"
                     /locus_tag="VNN25_16975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXH93279.1"
                     /translation="TSLAALASNDWKFDRKLPNDRTGTSPFGEKAAAELMFVNGMLYA
                     NRASLANEHPGIRIVPMREAMESESFAQHFARYADYEKHAMTALNTALWQDGTFIEIA
                     PGTVVEGFIHVCSISAPIKEHPVMTQQRNLIVAGRGSQITVVETFFGSGPYFTNTVTE
                     IVAGEGAVVDHYKYENESLDAFHIGTVQIHQERSSSVTSRTIAIGGGLVRNEINAALA
                     GEGASLSMEGIFVLSGRQHVDNHTVIDHVAPHCDSVELFKGVLDENARGIFDGRIIVE
                     PDAVKTNSRQTNKNLLLSETAVIDSKPSLEIHNDDVKCNHGSTIGQLDEEALFYLRSR
                     GISAEEARNLLVYAFASEIVERMKVGVVRETAAGALFLRMPGRMAEKLGRAEEVIN"
     gene            1174..2445
                     /locus_tag="VNN25_16980"
     CDS             1174..2445
                     /locus_tag="VNN25_16980"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXH93280.1"
                     /translation="MSAATETQAGPSSRVADSLDVDRIRRDFPILHRTVRENRKLVYL
                     DNAATTQKPQQVIDRIVRYYTDENSNVHRGVHYLSEVATAAYENARTTVKRFIGARDE
                     KEIVFTRGTTEGINLVAQTWGRENVGAGDEVLITAIEHHSNIVPWQMLCDEKGARLRV
                     APVNDSGELLIDEFQKLLTSRTKLIAFGHASNALGTINPIRKMIALAHAAGAVVLIDG
                     AQGVPHLHIDVRELDCDFYAFSSHKIYGPTGTGVLYGKEAILDAMPPWQGGGDMILSV
                     SFEKTTYNALPYKFEAGTPNIAGVIGMASAIDYMNAIGIERIAAHEQNLLHYATKRLL
                     ELDGLRIIGTAAEKACVISFVLEGVHPHDIGTILDDQGIAIRTGHHCAQPLMMRFNVP
                     ATGRASFAMYNTRDEADALVAGLEKVIEVFR"
     gene            2449..2898
                     /locus_tag="VNN25_16985"
     CDS             2449..2898
                     /locus_tag="VNN25_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXH93281.1"
                     /translation="MSDLRELYQQVILDHNKNPRNFHELAHPTRTVDGYNPLCGDHYT
                     IFVEMDGETIKDIGFTGNGCAISKASASVMSSTLKGKTTQEAHQLFDVFHKLVTGEPG
                     SLSTAELGRLAAFSGVSEFPARVKCASLPWHTLNSAIEGDKEVVSTE"
     gene            2984..>3196
                     /locus_tag="VNN25_16990"
     CDS             2984..>3196
                     /locus_tag="VNN25_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HXH93282.1"
                     /translation="MNIREEVTLSRSAEAVMIPSGEKVLVPEGARATITQSLGGSYTL
                     ITERGLMVRISGKEVEAIGKTPQTVPG"
BASE COUNT          680 a         1017 c          951 g          548 t
ORIGIN      
        1 cacctcgctt gcggcgctcg ccagcaacga ctggaaattc gatcggaagc tgccgaacga
       61 ccggacaggt acctcgccat tcggtgagaa ggccgctgcg gagctgatgt tcgtcaacgg
      121 aatgctctac gcgaaccgcg cctcgcttgc caacgagcat cccggaatca ggatcgtacc
      181 gatgcgcgag gcgatggaga gcgaatcttt cgcgcagcat ttcgcgcgct atgccgacta
      241 cgagaagcac gccatgaccg cgctgaacac ggcgctctgg caggacggca ccttcatcga
      301 gatcgcgccc ggaaccgttg tcgaaggctt cattcacgtt tgttccatct ccgcaccgat
      361 caaggaacat ccggtgatga cgcaacagcg caacctgatc gttgccggcc gcggaagcca
      421 gatcacggtc gtcgagacgt tcttcgggag cgggccttac ttcacgaaca cggtcacgga
      481 gatcgtcgcc ggcgaaggcg ccgtcgtcga tcactacaaa tacgagaatg aatcgctcga
      541 cgccttccac atcggcaccg tgcagattca ccaggagcgt tcgtcgagcg tcacgtcgcg
      601 cacgatcgcc atcggcggcg gtctggtgcg taatgaaatc aacgcagcgt tggccggcga
      661 aggagccagc ctttcgatgg aagggatctt cgtcctctcc ggccgccagc acgtcgacaa
      721 tcacaccgtg atcgatcatg ttgcgccgca ctgcgacagt gtcgagctct tcaaaggcgt
      781 cctcgacgag aacgcccgcg gcatcttcga cggccgcatc atcgtcgagc cggacgcggt
      841 gaagacgaat tcgcggcaga cgaacaagaa cctgctgttg tcggagaccg ccgtcatcga
      901 ttcgaagccg tcgctcgaga tccataacga cgacgtgaag tgcaatcacg gctcgacgat
      961 cggacagctt gacgaggaag cgctttttta ccttcgctcg cggggtataa gcgcggaaga
     1021 agcgcgcaac ttgctcgtgt acgcatttgc cagcgagatc gtcgagcgga tgaaggtcgg
     1081 cgtcgtgcgc gagaccgcag cgggcgcact gttcctgcgg atgccgggcc gtatggcgga
     1141 gaagctcgga cgagccgaag aggtgatcaa ttgatgagcg ccgctacgga aacgcaagct
     1201 ggtccgtcgt ctcgcgtcgc cgattcactc gacgtcgatc gcatccgccg cgacttcccg
     1261 atcctgcatc ggaccgtgcg cgaaaaccgc aagctggtct atctcgacaa cgccgcgacg
     1321 acgcagaaac cgcagcaggt catcgaccgc attgttcgct attacaccga cgagaactcg
     1381 aacgtgcacc gcggcgttca ctacctcagt gaagttgcca cggccgcgta cgagaatgcg
     1441 cgcacgacgg tgaagcgctt catcggcgcg cgcgatgaaa aagagatcgt cttcacgcgc
     1501 ggcacgaccg aggggatcaa tctggtcgcg cagacgtggg ggcgcgagaa cgtcggtgcc
     1561 ggggacgagg tgctgatcac tgcgatcgag catcactcca acatcgtgcc gtggcagatg
     1621 ctctgcgacg aaaagggagc gcgtctgcgc gtcgcgccgg tgaacgattc cggcgaactt
     1681 ctcatcgacg aattccagaa gttgctgacc tcgcgtacga agctcatcgc cttcggccac
     1741 gcttccaacg ccctcggcac tatcaatccg atccggaaaa tgatcgctct ggcgcacgca
     1801 gcgggcgcgg tagtcctgat cgacggcgca cagggcgttc cgcatcttca catcgatgtg
     1861 cgcgagctcg actgcgactt ctacgccttc tccagccaca agatttacgg tccgaccggc
     1921 accggcgtgc tatacgggaa agaggcgatt ctcgatgcca tgcctccgtg gcagggcggc
     1981 ggcgacatga tcctctcggt cagcttcgag aagacgacgt acaacgcgct cccctacaag
     2041 ttcgaggcgg gtacgcccaa catcgccggt gtcatcggca tggcctcggc catcgattac
     2101 atgaatgcga tcgggatcga gcgcatcgcc gcgcacgagc agaatctgct gcactacgcg
     2161 acgaagcggc ttctcgagct cgacggattg cgcatcatcg gcaccgcggc ggagaaggcg
     2221 tgcgtcatct ccttcgtgct cgaaggcgtt cacccgcacg acatcggaac cattctcgat
     2281 gatcagggga tcgcgatccg caccggccat cactgcgcgc agccgctgat gatgcgtttt
     2341 aacgttcccg caaccggccg cgcgtccttc gcgatgtaca acacacgaga cgaggccgac
     2401 gcgctcgtcg ccggtctcga aaaagtcatc gaggtattcc gctagaacat gtccgacctt
     2461 cgtgagctct accagcaggt catcctcgac cacaacaaga accctcgcaa ttttcacgag
     2521 ctggcccatc cgacgcgaac cgtcgatggc tacaacccgc tctgcggcga tcactacacg
     2581 atcttcgtcg agatggacgg cgagacaatc aaagacatcg gcttcaccgg caacggctgc
     2641 gcgatctcga aagcctccgc gtccgtgatg agctcgacat taaaaggaaa gaccacgcag
     2701 gaagcgcatc aactcttcga cgtcttccac aagcttgtga ccggcgagcc cggcagcctc
     2761 agcacggcag aacttggccg cctggccgcg ttctcaggcg tgtcagagtt cccggcgcgc
     2821 gtcaaatgcg cctcgctgcc ctggcacacg ctgaacagcg cgatcgaagg ggataaggaa
     2881 gtggtaagca cggagtaggg agtggggagt aggggtgggg agtgggagtg ggcggtcgtc
     2941 tccaactccc acccccccca actcccaacc cccaataaaa actatgaaca tccgcgaaga
     3001 agtcaccctc tcccgcagcg ccgaagccgt catgattccg agcggcgaga aagttctcgt
     3061 ccccgagggg gcgcgcgcca cgattacgca atcgctgggc ggctcctaca cgctcatcac
     3121 cgaacgcggt ctcatggtcc gcatctccgg caaggaggtc gaggccatcg gcaagacgcc
     3181 gcagactgtg cccggc
//
LOCUS       DATLVW010000576        11229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_193750, whole genome shotgun sequence.
ACCESSION   DATLVW010000576 DATLVW010000000
VERSION     DATLVW010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193750"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..547
                     /locus_tag="VNN25_16995"
     CDS             347..547
                     /locus_tag="VNN25_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93283.1"
                     /translation="MSKVEQIEREVEELSPEEWAAFRSWFVERDWQAWDRELERDVAE
                     GRLDRFAAEALAEFERGETTEL"
     gene            complement(820..1491)
                     /gene="radC"
                     /locus_tag="VNN25_17000"
     CDS             complement(820..1491)
                     /gene="radC"
                     /locus_tag="VNN25_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXH93284.1"
                     /translation="MHELIADLPFDERPRERMLMHGAETLSDAELLAVILGSGMRGKN
                     AIQLARELLNDGFASLARCDAEQLVKRNGVGEAKATRVVAAFEIARRLANFKTDDPRD
                     FDAGLFGRNLVIRSARLRQERLGALLLDSRHRVIRDRTLYVGTVDRAFVSAREVVRLT
                     LDANAVGVVLYHNHPSGDPTPSLEDNKYTKKTGETLRAIDIELVDHIIAGAHSFVSMG
                     QKGLI"
     gene            complement(1669..2643)
                     /locus_tag="VNN25_17005"
     CDS             complement(1669..2643)
                     /locus_tag="VNN25_17005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXH93285.1"
                     /translation="MKKTILALALTLSAASLSAAQLLEAIVIRVGDRVITRTQYVRRL
                     HDASTEIEQTATSPAEVATKKEELRKNLSNDLIAELLIKDRADRLGITVTPDEIKEAL
                     VRLKEQYGMKTDKDFEDSLRKSGMSRADMEARLHDTILTNKVFGRELRSREDLTDKEL
                     RDRYDREKERYRLPERARLREIIVIKPDDPTKVEAARERATALAAQSRTGDFAKLATT
                     SSDSGTKEKGGDLGEVARGELLPDLDKAVFNAQSGAVLGPIETKSGWHILKVEARLPS
                     EVPAFESVKDRLRKDASEDAWQRDYKAYIERLRKDAFIQINDQYIPST"
     gene            complement(2714..3604)
                     /locus_tag="VNN25_17010"
     CDS             complement(2714..3604)
                     /locus_tag="VNN25_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXH93286.1"
                     /translation="MDRRALLLVAFVLIAACHRKPSVPPDVVARVGERMVTLSDFKRY
                     LDRNTGTDLAQLGPEVASAMVDQYLEEVILSEYAAAHGVEIPAEKIASAVRTDAGVTV
                     IEKRDEMRRQRLIGDTSENVAPPSDADIRAYYEQHPAEFKSGEEVHVKQILVHDENVA
                     TEIDQKLHAGGSFEALSSQYSLAPNAKKGGEIGYVSRGELPKMFEDEIFALKPGNISG
                     VIRTDSSFHVFKVEDRRPPGVIDLATASPVIRERLKDDAVRDRMAQLVGRARSELSIA
                     VLTKRLPFKYTGGFPKSENE"
     gene            3690..4655
                     /locus_tag="VNN25_17015"
     CDS             3690..4655
                     /locus_tag="VNN25_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93287.1"
                     /translation="MKTTTLFFAVSAFAMTLAARQPSRLAHLSAADPATLVFSSVEPE
                     LPAQLLAQLHARKVTHAKGVRASAITDSISARAFIIPAAGSVAGGGGLFFKSDVTLVN
                     YRDTPQQVVVGFWAQGATNALNLASYKSVTLPSQQFITIQDFVANSLGASGLGSLVFI
                     PYTGTTLDANAAIDGFSRIYTKQPGSAGTVSQPFDAIDPDTLSVQYIDEGIALGLRQD
                     ADFRTNFGIVNVDPSPHAYKVSFVGEKLQTNITLTVPAYGMIQQAIPAGDYGALQLLY
                     QVTDAGNNNVSWIGYASSTDNVTGDGWVSIASANFSPDELTLIGY"
     gene            complement(4771..8307)
                     /gene="mfd"
                     /locus_tag="VNN25_17020"
     CDS             complement(4771..8307)
                     /gene="mfd"
                     /locus_tag="VNN25_17020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXH93288.1"
                     /translation="MTERNDRLLAQVRAELARTKAFRAIGKRIAEERLDITGTAVEAR
                     ALLLATLQDSTAQRMAIVVPGDAAIDDFESSLKLFHRDPQCVSSYPSPSLSPYQDLSP
                     SLGVARNEIRALGMLIDRSVDVLLVPARALFSRLPHAEEFAARVVKVAEGDELDLRVL
                     LERLVENGFVRTDLVGEAGEFAFRGGILDLFPPNTAKPVRVELFGDTVDSLRWFDVET
                     QRSEEASGSVTVLPMTQFPVTQPMRSALARRLSLDFNDPIYKRDLSDKIERINENGTF
                     PGMEHYLPVATNTVSFFDYLDPAEWMLALIEPDQITTNVAKFESLLSNEYEAASEKGR
                     AVYRPEKLTTAGTDVLALLGTARIAFSEVHVGAPHDRRRDAAGPAGGTAALQPDFEEL
                     RLRAPQVDRYTNRLPEFTNDVKRGHEKGRRQVFFAATKGGREKVERLLKEFDVVFVDD
                     AAEGAGTKGKAELGSEILLAAGALPRGFDFEEPNLTIYSEWDLFEPPTSTRVGGKKRT
                     TEAFVTDLRDLKVGDYIVHVDHGVGRFHGLQRIPFGPTEREVMEIEYSGGGKLLMPME
                     NLNLIQRFSGGADTQPKLDRLGGTAWARTKASVKKAMRDMADELLKLYATRQMVQGHA
                     FSKDSPWQFEFEDAFEFDETEDQESAIDDVKSDMESRKPMDRLLCGDVGYGKTEVAMR
                     AAFKSVMDGKQVAVLAPTTVLTYQHYRTFLRRFASFPVTIELLTRYYTPKEQKEILRK
                     LETGEIDIIIGTHRVLSKDINFKDIGLLVIDEEQRFGVGQKEKLKQIKKAVDVLSMSA
                     TPIPRTLHMSMVGIRDISVIETPPKDRLAIQTAVVPFSDDFIREAIEFEVGRGGQVFF
                     VHNRVESIYAMKEYLEKLVPGLRVVVGHGQMEERELERAMLAFIQREYDVLLATTIIE
                     NGIDIPACNTILINHAERFGLSQLYQLRGRVGRSDRLAFCYLLVPSDKVLTSDARKRL
                     AAIQEFSDLGAGFRIAAKDLEIRGAGNILGGEQSGQIAAVGFEMYTKLLEETIREMKG
                     ERIEEEVETSMNLGVDIYIPKNYISEENLRMTFYKKIASASTDDRLEQIRNEMRDRFG
                     ALPANVENLLHFVQVKRFAQQMGVISIVREGARAVVKLTAQAKVDPNKLLMLIQQNPQ
                     VKFTPNGVLIFPLKVQGPEVIDEIEATLKGLA"
     gene            complement(8479..9900)
                     /locus_tag="VNN25_17025"
     CDS             complement(8479..9900)
                     /locus_tag="VNN25_17025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH93289.1"
                     /translation="MRHQQSRKLLYLSYTTVTFLLFATAARAAAPTQLILQRDRQANL
                     TEDIKGPIDLTIVPGIDDARVTVSVDGQKLVEGLRSPWHVPVDFGPLPVQHKIAIAAV
                     GADRQRVQWQTMVNKGHQTLGVKIEPVDVANRIFEVNATSPDEDPIAEVSVWDGGQAV
                     VTVKEPPYRFTIPAEAFAQRSVQVTARTKSGAEAADFWSAAGDVKSESLDVRTVPLFV
                     SVVDNNGTARDDIDQALFKIVDNGTEAKILQIGKAFDQPISIALLLDASASMLYEMPK
                     ATRAAARFVEGTLKPGDRCAVFSIRSTPRREVALTSDLAAVGQAVKNLKASGQTALYD
                     AISTAERELRDEKNRRAIVILTDGGDTSSIATLDETDRLTKEAGVPIYVIAYQSGDPD
                     DDPHDLNRLQYLAGETGGFLVTASAQNLQAKYTDIEKDLRAQYAIVYQISDLARHNQW
                     RKVHVMLKSPTLTARTIRGYFAP"
     gene            complement(9878..>11229)
                     /locus_tag="VNN25_17030"
     CDS             complement(9878..>11229)
                     /locus_tag="VNN25_17030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXH93290.1"
                     /translation="YRGVRLRALFNSRRYTDLLAETAHVQLTDPSLILTRARAAWRAN
                     QPDAFLAGLRQVEKRFPKTSEAAEAKILRAKYYVTDEVNYDLAISNLEAAIAAGDLGN
                     EGENLWTLGWTYFRAGRDDDALHTFGRYMTQYPDGDYLSNSLFWAGKIHDRRGKRELR
                     DTAFHELEMRYPYSYFSFRARQIAGEPVLAPNEVTNGNVFPDIDGQLASVNEPRLDSV
                     RELAWLGLYREATREMKSLAAAYPTNLGMQFMLADLYVQGGEPFSANNVLQKKFRQFV
                     RHGGTNIPHRFWEILFPLNYWDTIKSESEKRQVDPYLMASIIRQETGFEPTTVSNADA
                     VGIMQIMPAEAAKIATAAGLPAPTREQLFDPKVNIAIGVAEYTQKLAAVHGNQMLAIA
                     AYNAGEDAVGKWIAQTPVDDEDLFVESIPFAETRLYVKTVSRNRYEYRRIYEAPAKP"
BASE COUNT         1955 a         3408 c         3574 g         2292 t
ORIGIN      
        1 tctaccatct tccaagaatc aagatgacat ttcagcgcta cggtgccgac gtctggacgg
       61 ttccctgcaa cacctcggtg cttgcggatt tgccgttcaa cctgttgcga gaggacgctg
      121 ctttctgggt gtattacctc cggcggctgc gctagaaatc gactcaaagc atccgtctgt
      181 gccatcgaga cgaagctgta cccccggagc ctcccagcgc tgccgaaacc ggcgctggtg
      241 gcggggtagg cgaacggcga ccggcgttga ctgccctcgg cattctgatt aagaaggata
      301 gttgggcggt ggaaagagcc gagaagccgc tatcattcaa atcgtcatga gcaaggtgga
      361 gcagattgag cgcgaagttg aggagttgag cccggaggag tgggccgcgt tccgctcgtg
      421 gttcgttgag cgcgactggc aggcatggga tcgagagctt gaacgtgatg tggccgaggg
      481 gcgtctcgac cggtttgcgg ccgaggctct ggcagaattt gagcgcggtg aaactactga
      541 gctttgagtc atcgtgcttc aagtttgttt tggcggcgtt tcgccgagct tccacctgaa
      601 ttgcaggctt tggcccgcgc caactacgaa ctgcttgaag tccaaccctc gtcatccttc
      661 gcttcaattc aagcgagtcg gtccctacgg gtcagcgcgc gtgggctcat cgcatcgtgt
      721 gctcggcgcg gtctcgtcaa ccggcatcgt gtggttctgg attgggccgc acgaagagta
      781 cattcgcctt atccggtagg cctgagcctc aactcctcct caaattaacc ccttctgccc
      841 catcgaaaca aagctgtgcg cgccggcgat gatgtggtcg acgagctcga tgtcgatcgc
      901 gcgaagggtc tcgcctgttt tcttggtgta cttgttgtct tcgagggagg gtgtcgggtc
      961 gccggacgga tggttgtggt acaggacgac gccgacggca ttggcgtcga gcgtgagacg
     1021 aacgacttcg cgcgcggaga cgaaggcgcg gtcgacggtg ccgacgtaga gggtgcggtc
     1081 gcggatgacg cggtggcgcg agtcgaggag gagggcaccg agccgctcct gacgcagccg
     1141 tgcggatcgg atgacgaggt tgcggccaaa gaggccagca tcgaagtccc gcggatcatc
     1201 ggtcttgaag ttcgccaggc gtcgtgcgat ctcgaacgct gcgacgacgc gcgtcgcttt
     1261 cgcctcaccg acgccgttcc gtttcaccag ttgctccgca tcgcagcgag cgagtgatgc
     1321 aaagccatcg ttgagaagct cgcgcgccag ttggatggcg ttctttccgc gcatgccgga
     1381 cccaaggatc acggcgagca gttcggcatc ggagagggtt tccgcgccgt gcatcagcat
     1441 ccgctcacgc gggcgctcat cgaaaggaag gtcggcaatc agctcgtgca ttggtgccac
     1501 gaagcttaag ccgcaagaca acaatacacg tcaaactcta ctggggcggg ggataacggc
     1561 tgtccgaggg gcgaaaagtg cctggaaaag ccaccgggtg tagatcgcgc aaaggaaaag
     1621 ggcggactgg agaagtccgc ccttcgatga ttctctctgc gtcgcgagct acgtcgacgg
     1681 gatgtactga tcgttgatct ggatgaacgc gtctttgcgc agccgttcga tgtaagcctt
     1741 gtagtcgcgc tgccaggcgt cttcgctcgc atccttccga aggcggtctt tgacggactc
     1801 gaacgccgga acttcggacg gcaggcgtgc ttcgactttc aggatgtgcc agccggactt
     1861 cgtttcgatc gggcccagga cggcgcctga ttgggcattg aacacagctt tgtcgagatc
     1921 ggggaggagc tcgccgcgtg cgacttcgcc aaggtcgccg cccttttctt tggtgccgga
     1981 atcggaggag gtcgtggcca gcttcgcgaa gtcgccggtg cggctctgcg ccgcaagcgc
     2041 ggtagcgcgc tcgcgggcgg cttcgacttt ggtcggatca tccggcttga ttacgatgat
     2101 ctcgcgcaga cgggcgcgct ccgggaggcg gtagcgctcc ttctcgcggt cgtaacggtc
     2161 gcgcaattcc ttgtcggtca gatcttcgcg cgaccgcagc tcgcggccga agaccttatt
     2221 ggtcaggatc gtgtcgtgaa ggcgcgcttc catgtcggca cgtgacatcc cggacttgcg
     2281 gagcgagtct tcgaaatcct tgtccgtctt catgccgtac tgctctttca ggcggacaag
     2341 ggcttctttg atctcatcgg gggtaactgt gatgccgagc cggtcggcgc ggtctttgat
     2401 cagcagttca gcgatcagat cgttcgagag attcttccgc aactcttctt tcttcgttgc
     2461 gacttcggca ggagacgtgg ccgtctgctc gatctccgtg gaggcgtcat gcagcctgcg
     2521 cacgtattgc gtccgtgtga tgacgcggtc accgacgcgg atgacgatcg cttctagtaa
     2581 ctgggcggcg gaaagcgagg ccgccgagag ggtaagggcg agtgcaagaa tcgttttttt
     2641 catgcgtatc tctttgacgt agatggaggg tttgggttcc tgtacggacg gacaacggcg
     2701 gtacggagcc ggtttattcg ttctccgact tcgggaaccc gccggtgtac ttgaaaggga
     2761 ggcgcttggt caggacagcg atcgaaagct cggagcgggc ccgtccgacg agctgagcca
     2821 tgcgatcgcg gacggcgtcg tctttcagcc gttcgcggat gaccggtgag gcggtggcga
     2881 ggtcgatgac accggggggg cgccggtctt ccaccttgaa gacgtggaag ctggaatccg
     2941 tccggatgac gccgctgatg ttgcccggct tgagcgcgaa gatctcgtcc tcgaacattt
     3001 tcggcagctc gccgcgactg acgtagccga tctcgccgcc tttcttcgca ttcggggcca
     3061 gcgagtattg gctggagagt gcctcgaacg agccgccggc gtgcagcttc tgatcgatct
     3121 ccgtggcgac attctcgtca tgcacgagga tctgtttgac gtgcacttcc tcgccgctct
     3181 tgaactcggc gggatgctgc tcgtagtaag cacggatgtc tgcgtctgac ggcggtgcga
     3241 cgttctccga tgtatcgccg atgagccgct ggcgcctcat ttcgtcgcgt ttttcgatga
     3301 cagtgacgcc ggcgtcggtg cggacagcgg aggcgatctt ttccgccgga atctccacac
     3361 cgtgagccgc tgcgtactcg gagaggatca cttcttccag gtactgatcc accatcgcgc
     3421 tcgcgacctc ggggcccaat tgcgccagat cagtgccggt gttccggtcg agatagcgct
     3481 tgaaatcgct gagcgtgacc atccgctcgc ccacgcgcgc gacgacgtcc ggcggcaccg
     3541 acggtttgcg gtggcaggcg gcaatcagga caaaggcaac aaggagaaga gctcgacgat
     3601 ccacgaggca ctcaaagtgt agccgattcg cgtttgcgcc gcgaccgcat gcggaatcag
     3661 aatggactgt ttccggagga acccgaccca tgaaaacgac caccctgttc ttcgcagtga
     3721 gcgcattcgc gatgacgctg gctgcacgcc agccgtcgcg tctggcgcac ctctccgccg
     3781 ctgaccctgc caccctcgta ttctcgtccg tcgagccgga gttgcccgcg caactcctcg
     3841 cacagctcca cgcccgcaaa gtcacccacg cgaaaggtgt gcgcgcatca gcgattaccg
     3901 actccatctc ggcgcgcgcg ttcatcattc ctgcggccgg tagtgtggct ggcggcggcg
     3961 gccttttctt caagtctgat gtcacgctcg tgaactatcg cgacacgccg cagcaggtag
     4021 tcgtcggctt ctgggcgcag ggcgcgacca atgcgctgaa ccttgcgagc tacaagagcg
     4081 tcacgcttcc ttcgcaacaa ttcatcacga tccaggactt cgtggccaat tcgctcggag
     4141 cgtccggcct cggcagcctg gtattcatcc cctacaccgg cacgaccctc gacgccaacg
     4201 cggcaatcga tggcttctcg cgcatctaca cgaaacaacc gggatcggcc ggcacggtat
     4261 cgcagccgtt cgatgctatc gatcccgata cgttgtccgt gcagtacatc gacgagggaa
     4321 tcgcgctggg cctgaggcag gatgcggatt tccgcacgaa cttcggcatc gtgaacgtcg
     4381 acccatcgcc gcatgcctac aaggtcagct tcgtcggcga gaagctgcag accaacatca
     4441 ccctcacggt ccccgcatac gggatgattc agcaggccat tcccgcgggt gactacggcg
     4501 cgctgcagct cctctatcaa gtgaccgatg caggcaacaa caacgtgagc tggatcggtt
     4561 acgcgagcag cacagacaac gtcaccggcg acggctgggt atcgatcgct tccgctaact
     4621 tttcgccgga cgaactgacc ttgatcggat attgatcttg cgcttcgcgc gttatcggtt
     4681 gtcgctgttc ggttggttgt gtcacttcgc gacgatggtg acaaccgaca actgagaacc
     4741 gacaacgcgg cgcgcccagc gcgccgcaat tcaagcgagt cccttcaatg tcgcctcgat
     4801 ctcgtcgatc acctcgggtc cctgcacctt gagcgggaag atcagcacgc cgttcggggt
     4861 gaacttcacc tgcgggtttt gctggatgag catgagcagt ttgttcggat cgaccttggc
     4921 ctgtgccgtc agcttcacga ccgcacgcgc gccttcccgg acgatcgaga tcacgcccat
     4981 ctgctgcgcg aagcgcttga cctgcacgaa gtgaaggagg ttctcgacgt tcgccggtag
     5041 tgcgccgaag cggtcgcgca tctcgttgcg gatctgttcg aggcgatcgt ctgtcgatgc
     5101 cgaggcgatc ttcttgtaga acgtcatgcg caggttctct tcgctgatgt agttcttggg
     5161 gatgtagatg tcgacgccga ggttcatgga ggtttccact tcctcctcga tgcgctcccc
     5221 cttcatctct cggatcgtct cctcgagcag cttcgtgtac atctcgaagc cgacggcggc
     5281 gatctggccg gactgctcgc cgccgagaat gttgccggcg ccgcggatct cgaggtcttt
     5341 cgcggcgatg cggaagccgg cgccgaggtc ggagaactcc tggatcgcgg cgaggcgctt
     5401 gcgggcgtcg gaggtgagaa ctttgtcgct cggcacgagc agatagcaga acgccagccg
     5461 gtcgctgcga ccgacgcggc cgcgaagctg gtagagctgc gacaggccga aacgctccgc
     5521 gtgattgatg aggatcgtgt tgcaggcggg gatgtcgata ccgttctcga tgatcgtcgt
     5581 tgcaaggagc acgtcgtatt cgcgctggat gaaggcgagc atggcccgct ccagctcgcg
     5641 ctcctccatc tgcccatgtc cgacgacgac gcgcagaccg ggaacgagct tctccagata
     5701 ctccttcatc gcgtagatcg actcgacgcg gttgtggacg aagaagacct gcccgccgcg
     5761 tccgacctcg aactcgatcg cttcgcggat gaagtcgtcg ctgaacggca cgaccgcggt
     5821 ctgaatcgcc agccggtcct tgggtggcgt ctcgatgacg gagatgtcgc ggatccccac
     5881 catcgacatg tgcagcgtgc ggggaatggg cgtggcggac atcgagagca cgtcgaccgc
     5941 tttcttgatc tgcttgagct tttctttctg gccgacgccg aagcgctgtt cttcgtcgat
     6001 gactaaaagg ccgatatcct tgaagttgat gtccttagac aggacgcggt gcgtgccgat
     6061 gatgatgtcg atctcgcccg tctccagttt cctgaggatc tccttctgtt ccttcggcgt
     6121 gtagtagcgc gtgagcagct cgatggtcac cggaaacgag gcgaagcggc gcaggaacgt
     6181 gcggtagtgc tggtaggtga ggacggtggt cggcgcgagg acggccacct gcttgccatc
     6241 catcaccgat ttgaacgccg cgcgcatcgc cacttcagtc ttgccgtaac cgacgtcccc
     6301 gcagagcaag cggtccatcg gtttgcgcga ttccatatcc gacttcacgt catcgatcgc
     6361 cgattcctga tcctcggtct catcgaattc gaacgcatcc tcgaactcga actgccacgg
     6421 tgagtccttc gagaatgcgt gcccctgcac catctgccgc gtggcgtaga gcttgagcag
     6481 ctcgtcggcc atgtcgcgca tcgccttctt gaccgacgct ttcgtgcgcg cccaggccgt
     6541 gccgccgagg cggtcgagct tcggctgcgt gtcagcgccg ccggagaagc gctggatgag
     6601 attcaggttc tccatcggca tcagcagctt gccgccgccg gagtactcga tctccatcac
     6661 ctcgcgctcg gtcggcccga acgggatgcg ctgcaggccg tggaaccgcc ccacgccgtg
     6721 atcgacgtga acgatgtagt cgccgacttt caggtcgcgc aggtcggtga cgaacgcctc
     6781 ggtcgtgcgc ttcttcccgc cgacgcgcgt cgaagtcggc ggctcgaaga gatcccactc
     6841 cgagtaaatg gtgaggttcg gttcttcgaa gtcgaagccg cggggaaggg caccggcagc
     6901 gagaagaatc tcgctcccca gctcggcttt gcccttggta cctgcgccct cggcggcgtc
     6961 atcgacaaag acaacgtcga actccttcag cagccgctcg accttctccc gcccaccttt
     7021 cgtggccgca aagaaaacct gccgccgccc tttctcgtgc ccacgcttca catcgttcgt
     7081 gaactcaggc agccggttgg tgtaccggtc aacctgcggc gctcgcagcc gcaactcctc
     7141 aaagtccggc tggagcgctg ccgtcccgcc ggctggcccg gcggcgtctc gccgccgatc
     7201 gtgcggcgct cccacatgca cctcactgaa cgcaattcgc gccgtcccca gtaacgccag
     7261 cacatccgtc cccgctgtcg tcaacttctc cggccggtaa accgcgcgcc ccttttcgga
     7321 cgccgcctcg tactcgttgg atagcagcga ctcgaacttc gccacgttcg tcgtgatctg
     7381 gtccggctcg atgagcgcga gcatccattc ggcaggatcg agatagtcga agaacgagac
     7441 ggtgttggta gccaccggca ggtagtgctc catgcccgga aaggtgccgt tctcgttgat
     7501 gcgctcgatc ttgtcggaga gatcgcgttt gtagatcgga tcgttgaagt cgagcgacag
     7561 gcggcgcgcc agcgcgctgc gcatcggctg cgtgaccggg aactgcgtca tcggcagaac
     7621 ggtcaccgag ccactggcct cttccgagcg ctgcgtctcg acgtcgaacc agcgcagcga
     7681 atcgacggtg tcgccgaaaa gctccacgcg caccggcttg gcggtgttgg gcggaaagag
     7741 atcgaggatg ccgccgcgaa acgcgaactc gcccgcctcg ccgacgagat ccgtgcgcac
     7801 aaaaccgttc tcgacgagcc gctccagcag cacgcgcaaa tccagctcgt ccccctcggc
     7861 caccttcacg acgcgcgcgg caaactcctc ggcatgcggc agccgcgaaa acagggcacg
     7921 cgccggcacg agcagcacgt cgacgctccg gtcgatcagc attcccagcg cccgaatctc
     7981 gttgcgcgcc acgccgagcg atggcgacag atcctgatac ggcgagagcg aaggggatgg
     8041 atacgacgac acgcactgcg gatcgcgatg aaacagcttc agcgaggact cgaagtcatc
     8101 gatggccgca tcgcccggaa cgacaattgc catccgctgc gctgtcgagt cctgaagcgt
     8161 cgccagcagc agtgcccgtg cctcgaccgc ggtgccggtg atgtcgagcc gttcctccgc
     8221 gatcctcttc ccgatcgcgc gaaacgcctt cgtccgcgcc agctccgcgc ggacttgcgc
     8281 cagcagcctg tcgtttcgct cagtcatgcg ccggggattc tatgcgcggc gaacgaacat
     8341 ctcgcacctg cgtgcgcgcc gaagcaaagg caaccacaga gacacagaga acacagaggg
     8401 aacctccgtg acctctgtgc ctctgtggtg atcggttttt caaattgacc cactacccag
     8461 agacgactct cgctcttcct acggcgcgaa ataccccctg atcgtcctcg ccgtcagcgt
     8521 cggcgatttg agcatcacat gcaccttgcg ccattggttg tgtcgtgcca ggtcgctgat
     8581 ctgatagacg atcgcgtatt gcgcgcggag gtctttttcg atgtcggtgt atttggcctg
     8641 gagattttgg gccgaggcgg tgacgaggaa accaccggtt tcgccggcca ggtattggag
     8701 gcggttcagg tcgtggggat cgtcgtcggg atcgccgctc tgataggcga tgacgtagat
     8761 ggggacgccg gcttccttgg tcaggcggtc ggtctcgtcc aatgtggcga tcgatgatgt
     8821 gtcgccgccg tcggtcagga tgacgatggc gcggcggttc ttctcatcgc gcaactcgcg
     8881 ctctgccgtg gagatggcgt cgtagagtgc ggtctgtccg cttgctttca gattcttgac
     8941 tgcctgtccg actgcggcca gatcgctcgt cagcgccact tcacggcgtg gcgtggagcg
     9001 tatcgagaag actgcgcaac ggtcgccggg tttcagcgtt ccttcgacga agcgggcggc
     9061 ggcgcgcgtc gctttcggca tttcgtaaag catgcttgcc gaggcgtcga gcagcagcgc
     9121 gatcgagatc ggctggtcga acgccttgcc gatctgcagg atcttcgcct cggtgccgtt
     9181 gtcgacgatt ttgaacaacg cctgatcgat gtcgtcgcgc gcggtgccgt tgttgtccac
     9241 gaccgagacg aacagcggaa ccgtgcggac gtcgagcgac tcgctcttga cgtcgccggc
     9301 ggccgaccag aagtcggcag cctcggcgcc cgacttcgtt cgcgccgtca cctgcacgct
     9361 tctctgtgca aaggcttcag ccggtatcgt gaaacggtat ggaggctcct tgaccgtcac
     9421 aaccgcctgc ccgccatccc aaaccgagac ctcggcgatc gggtcttcat ccggcgacgt
     9481 cgcattcacc tcgaagatgc ggttcgcgac atcgaccggc tcgattttca cgcccagggt
     9541 ttgatggccc ttgttgacca tcgtctgcca ttgcacgcgc tggcggtcgg cgcctacagc
     9601 ggcgatcgcg atcttgtgct gtacgggaag aggtccgaag tcgacgggga cgtgccacgg
     9661 cgagcgaagg ccttcgacaa gcttctggcc atcgacagag acggtgacgc gcgcatcgtc
     9721 gatgccggga acgatggtca ggtcgattgg acctttgatg tcttccgtga gattggcctg
     9781 gcggtcgcgc tggagaatga gctgcgtggg agcagcggcg cgtgccgcgg tggcgaaaag
     9841 gaggaaggtg accgtggtgt acgacaggta aagcaactta cggctttgct ggtgcctcat
     9901 aaatccgccg atactcgtat cggttccggc tgacggtctt tacgtagagc ctcgtttccg
     9961 caaacggaat cgattcgaca aacaggtcct catcatcgac cggcgtttga gcaatccatt
    10021 tgccaactgc atcttcaccc gcgttgtagg cagcgattgc cagcatttga ttgccgtgga
    10081 cggcggccag tttttgcgtg tattcggcca cgccgatggc gatgttgacc ttcggatcga
    10141 agagttgttc gcgcgtcggc gcagggagtc ctgccgcggt tgcgatcttg gcggcctctg
    10201 ccggcatgat ctgcatgatg cccacggcat cggcattgga gacggtcgtc ggctcgaatc
    10261 cggtctcctg acggatgatc gacgccatga ggtatggatc tacctggcgt ttctcggact
    10321 cgctcttgat cgtgtcccag tagttgagcg ggaagagaat ctcccagaag cgatgcggga
    10381 tgttcgtgcc gccgtggcgg acgaactgcc ggaacttctt ctgcagaacg ttgttggccg
    10441 aaaacggctc gccgccctgc acgtagagat cggcgagcat gaactgcatg ccgaggttgg
    10501 tggggtaggc ggcggcgagc gatttcatct cgcgcgttgc ctcgcgatag agccccagcc
    10561 acgccagctc gcgcacggag tcgaggcgcg gctcgttcac tgatgccagt tggccgtcga
    10621 tgtccgggaa gacgttgccg ttggtgactt cgttcggcgc cagcaccggc tcgcccgcga
    10681 tctggcgcgc gcgaaagctg aagtagctgt acgggtaccg catttcgagc tcatggaagg
    10741 cggtgtcgcg gagctcgcgc ttcccgcggc ggtcgtgaat cttccctgcc cagaagagcg
    10801 agtttgatag gtagtcgccg tcggggtact gcgtcatgta gcggccgaag gtgtggaggg
    10861 cgtcgtcgtc gcgcccggcg cggaagtagg tccacccgag agtccagagg ttctcgcctt
    10921 cgttgccgag gtcgccggcg gcgatggcgg cttcgaggtt ggagatggcc aggtcgtaat
    10981 tcacctcgtc cgtcacgtag tacttcgcgc gcaggatctt cgcctcggct gcttcgctgg
    11041 ttttcgggaa acgcttctcg acttgacgca atcccgccag gaacgcgtcc ggttggttgg
    11101 cacgccacgc ggcgcgggca cgcgtgagga tcagcgacgg gtcggtgagc tggacgtgcg
    11161 cggtctccgc aagcaggtcc gtgtagcggc gcgagttgaa gagcgcgcga aggcgcacgc
    11221 cgcgatagg
//
LOCUS       DATLVW010000577         7787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_234474, whole genome shotgun sequence.
ACCESSION   DATLVW010000577 DATLVW010000000
VERSION     DATLVW010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234474"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..379)
                     /locus_tag="VNN25_17035"
     CDS             complement(116..379)
                     /locus_tag="VNN25_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93291.1"
                     /translation="MEVVDRVRSALSNPQWDFRTIGGIARDTKLSREQVERVLADHQD
                     LFRQSHLTRNGAPLYTLREKPESLRERYAELRDFIASPVMYRR"
     gene            696..3599
                     /locus_tag="VNN25_17040"
     CDS             696..3599
                     /locus_tag="VNN25_17040"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH93292.1"
                     /translation="MSAIDPFVPGGTVLSYRLIERVSVTVWRAEDTRSGKPVAVKILT
                     KQLPRDPARREMVIRDMRQGAAIYHASLVTNIDVLPADDALILVMEWFEGRPVAAVFR
                     GHAAERTEFFHIAYQLADALKLMHAKNVLHLNIAGDSVLVADNGQVKIAGLNSMTFLP
                     RREGLGSAFALKGTDPDAVSYMAPEQIINQPVTVQTDVFSLGILMYEIATGRRPYLAP
                     SAAEIARKIVDEQPPSPKALNPAIDAAVLSVMGKCFYKDPYRRHKDMKAIIDDVVKAD
                     AEVVTWANEMGRAALHAVATARHAAQTRQSIILAGDIANFQEMNAVDPAGASKAAARM
                     QQILGEAIYLFDGQVVDPFGPRFVGELPSLESAFEAGRKAEFDFSPDQQDGTPIPVRL
                     LLHAGELETRDGSPAGATVDKAFEILDQIEPQKLYITEAVTKKGRATVRLRDARARAG
                     VKLFEIAPDIEVAENAAHAAAAADDSARLAAEAEAEAATAARAAQKKKRTQLAAIAIA
                     ALVILAAAAAFLWLRGRREEPAAVAIARHTGLPQASAATPRKVFIEPFSVTPADPALQ
                     QRADAIRLASIEVLRAFPELRIVDAKGSDVTAYTAALTVAGASPQIIAVTDGKVVKKG
                     TATPLLDAAGGIQAVVGWVAGDLKIPPRPPASAEAANAFADAVASHAVGDDTRAEVAL
                     RVALKADPNFLPTELLAFRFFDARGNGPDALAAAKQVLALDPTNVDAARHVARASLAA
                     GDLPAAFNGFGAVLRHDHSDAEALNTIGRYSLASGDHTLFKAALTRLGGSDASALHAP
                     DVLVSSGRIDNAVDMYYDLETHQPHNPALALKIGRIAVLRHSTEVAEVELKKVEQMDP
                     NYGAHILKAYIAAQSSNKTLADAEMKAASAASKPGDDYWTTAGEIAAMAGDTKGVDDA
                     LEHAAARKEPTASYVLANPLFGFMQSDARFAKLRETLKGQQNEIRSALAGLTI"
     gene            3611..4021
                     /locus_tag="VNN25_17045"
     CDS             3611..4021
                     /locus_tag="VNN25_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXH93293.1"
                     /translation="MIVTARLTFSAAHRLHNPGYDADWNRRIYDKCDNPKGHGHNYVI
                     TVSVKGKIDPETGMVIDLKRLKDIVRKQIIDRVDHTNLNEDVEFLHGVIPTAENLAQS
                     FWRQLAPAITEGALYEVVLQETEKNSVVYRGEDE"
     gene            4018..4251
                     /locus_tag="VNN25_17050"
     CDS             4018..4251
                     /locus_tag="VNN25_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93294.1"
                     /translation="MNYDLAMMALDGLMRTPLTVRTDSAGVTFTGEPAAFRELARLCL
                     LLGGASDADDSFELQPNVHVTADSPKVMLKLET"
     gene            complement(4280..4513)
                     /locus_tag="VNN25_17055"
     CDS             complement(4280..4513)
                     /locus_tag="VNN25_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH93295.1"
                     /translation="MPKLLRCREMLKRLRAHDKRFSVSVERGKGSHRMVVLTTKDGES
                     HYPFPCHNDGAEIDRRYLRDIINYFGLPPDIFD"
     gene            complement(4506..4868)
                     /locus_tag="VNN25_17060"
     CDS             complement(4506..4868)
                     /locus_tag="VNN25_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93296.1"
                     /translation="MSDEPKTFEVSVIAFREDSGWTALALEMNLRGYGSTVKKAIDDL
                     VEMLGAQVSFALQKGHPESVWNRADDKYWRMFEEGRRNRFVAEISGFEPPTDLVADMV
                     PLSLLAMQHGETWIAANA"
     gene            5142..7655
                     /locus_tag="VNN25_17065"
     CDS             5142..7655
                     /locus_tag="VNN25_17065"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXH93297.1"
                     /translation="MEILQFARSRRGGELIGIVILAAGICLALALLTYHADDSSAFYT
                     STNSGVANWIGYYGATVAWICVGFFGFASLLFPGALLVVGWNRFWVKEIEYLQTKAIG
                     FVILVAAMPPLCDLAFGKVWVRGAQIPSGGYLGQELNRAASGKLNSSGAGIVLVTAML
                     VGLLLATRISLAAVFRAIHQKLLGLGRAISLQWARFTERRRKDKMKTALVRKHLEKDA
                     PALRLVADDEMPSSMPGAGPIVREVKGAGRFQIRKVTKADLRKAAEELSKQPSHDPST
                     LWAEVQQQAEKPQLSRPVAVDDDGEFEDPLYLDDPPAVRPARPKQQMPRPVPRKLKEA
                     PPKRETRLTHDLLPPVDLLTEGPKQDTINDDVHKKFLDIGRLIEDRCKEFAVEGEVTA
                     YHPGPVVTTFEFKPSAGVKYAKVVNLGDDLALALKAESIRIERISGSSTVGIEVPNKK
                     RELITLRDIIDTDAFTQQSSLLTLALGKDIHGEAIVTDLGRMPHLLVAGATGAGKSVG
                     LNSMIVSLLYKALPKQLRMLMIDPKMVELKMYEDIPHLLHPIVTDPKLASNALIWAVN
                     EMENRYRCLAECGVRNIDQYNALLKDVDGYRRAMKRPEGELVKVPEPLVYIVIIIDEF
                     ADLMMVASKDVEDSVTRLAQKARAVGIHLIIATQRPSVDVITGVIKANFPSRISYQVA
                     SKIDSRTILDAQGAEKLLGNGDMLFLPPGTARVRRIHGAYVSEKEIVKIVDFVKANQG
                     EPQFLEEITKVVDEKSGADGIEYLDDPKYEDAVRVVLTTGQASASYIQRRLKLGYSRA
                     ARLIEIMEANGVVGPSQGSKPREILVRADDYPPASGQTI"
BASE COUNT         1536 a         2515 c         2344 g         1392 t
ORIGIN      
        1 aaccagtatt tccgccacgg caaggctgtt gtcggattgc ggcgatcttt gtaccaagtg
       61 agtgcctcgg cgatgaatcc gccgaagagg ccccacgaga atgcttcgat accgctcacc
      121 gacgatacat cactggtgat gcgatgaagt cgcgcaactc ggcatagcgt tcgcgcaaac
      181 tctccggttt ttcacgcaac gtgtagagag gtgcaccgtt gcgagtgaga tgggactgac
      241 ggaagagatc ctgatgatca gcaagaacgc gctccacttg ctcacgcgaa agtttggtat
      301 cgcgggcgat gccaccaatc gtgcggaagt cccactgcgg attgctcaaa gcgctgcgaa
      361 cacggtccac gacctccatc gtgctcgctt cgggtgtgga ccccccccta tctgctaaag
      421 cctgcgtggt cattggcttg gttctcgtga tttcaactcg cgatatagaa caattgaaac
      481 gccgcgcata atctacgctc acgccattcc ggatgtcaat gcgaccaaat gcatcaaaac
      541 gcgatggtta ggtcgttgac aggcacagcc actccggcag tctcagggat gacacctgac
      601 ttgatgggcc aagtcaaagc catcgagacg acagccggcc tcttctccca ccccccaccc
      661 ccgacacccc acaccccacg tctataattc acccaatgtc tgccatcgat ccgttcgtgc
      721 ccggtgggac tgttctctcg tatcggctga tcgagcgcgt tagtgtgacg gtttggcgcg
      781 cggaagatac gcgcagcggc aagccggttg ccgtcaagat tctgacgaag cagctgccgc
      841 gcgatcccgc ccggcgcgag atggtcatcc gcgacatgcg gcagggcgcg gcgatctatc
      901 acgcatcgct cgtcaccaac atcgacgtgc tgccggcgga cgacgcgctc attctcgtca
      961 tggagtggtt cgaagggcgg cccgtcgctg cggtctttcg cggacatgcg gccgaacgga
     1021 cggagttctt ccacatcgcc taccagcttg ctgatgcgct gaagctgatg cacgcgaaga
     1081 acgtgttgca tctgaacatc gccggcgatt ccgtgctcgt tgcggacaac gggcaggtca
     1141 agatcgccgg cctcaattcg atgacgttcc tgccgcggcg cgaggggctt ggctcggcat
     1201 tcgcgctcaa gggaaccgac cccgacgccg tcagctacat ggcgccggaa cagatcatca
     1261 accagccggt caccgtgcag accgatgtgt tctccctcgg catcctaatg tacgagatcg
     1321 caacgggacg ccggccctac cttgcgccca gcgcggcgga gatcgcccgc aagatcgtcg
     1381 atgaacagcc gccgtcaccc aaggcgctca atcctgcgat cgacgccgcc gtgctcagcg
     1441 tgatggggaa gtgcttctac aaggatccct atcgccgcca caaagacatg aaggcgatca
     1501 tcgacgacgt cgtgaaagcg gacgccgagg tcgtgacatg ggcgaacgag atggggaggg
     1561 ccgcgctcca tgccgtcgcc accgcccgtc acgccgcaca gacgcggcag tcgatcattc
     1621 tcgccggtga catcgccaac ttccaggaaa tgaacgcggt cgatcctgcc ggggcgtcga
     1681 aagcagccgc gcggatgcag cagattctcg gcgaggcgat ctatcttttc gacggccagg
     1741 tcgtcgatcc gttcgggccg cgtttcgttg gcgagttgcc gtcgctcgag agtgctttcg
     1801 aggcgggacg caaagcggag ttcgactttt ctcccgacca gcaggatggc acgccgattc
     1861 cggtgcgcct gcttcttcac gcgggcgagc tggagacccg tgatggatcg ccagccggcg
     1921 ctacggtcga caaagcgttc gagattctcg atcagatcga accgcagaag ctctacatca
     1981 cggaggccgt gacgaagaaa gggcgtgcga ccgtgcggct gcgcgacgcc agagcgcggg
     2041 ccggcgtgaa gctctttgag atcgcgccgg atattgaggt ggcggagaat gcggcccacg
     2101 cggccgctgc ggcggacgac tctgcgcgcc tcgccgcgga ggcggaggcc gaggcggcga
     2161 cggcggcgcg cgctgcgcaa aagaagaaac gtacgcaact ggcggcgatc gcgatcgctg
     2221 ctctcgtgat tcttgcggcc gcggcggcgt ttctttggtt gcgcggcagg cgcgaagagc
     2281 cggcggcggt ggcaatcgct cgacacaccg gtcttccgca ggcgtccgcg gccacgccgc
     2341 gcaaagtgtt catcgagccc ttctcggtca cgccggccga tcccgcgctc caacagcggg
     2401 cggatgcgat ccgtctcgct tcgatcgaag ttcttcgcgc ctttcccgaa ctgcgcatcg
     2461 tcgatgcgaa gggttccgat gtcaccgcgt acaccgcggc gctgacggtc gcaggcgcct
     2521 cgccacagat catcgccgtc accgacggaa aggtcgtgaa gaagggaacc gccaccccat
     2581 tgctagacgc cgccggcggg attcaggcgg tggtcggctg ggtcgccggc gatctgaaga
     2641 ttcccccgcg gcctcccgcc agcgccgagg cggccaacgc gtttgcggac gccgtggcct
     2701 cgcatgccgt cggcgacgat acccgcgccg aggtcgcgct gcgtgtcgcg ctcaaggcag
     2761 atccgaactt cctgcccacc gagctgctcg cgtttcgctt cttcgatgcg cgcggcaacg
     2821 gcccggacgc gctcgcggcg gcgaaacagg tcctggcttt ggatccgaca aacgtggatg
     2881 cggcgcggca cgtcgcgcgt gccagtctcg ctgccggcga tctccccgcg gcattcaacg
     2941 gctttggcgc ggtgctgcgt cacgaccatt ccgacgcgga agcgctcaac accatcggtc
     3001 gctactccct tgcttccggc gaccacaccc tcttcaaagc cgctcttacc cgcctcggtg
     3061 gatcagacgc cagtgcgttg catgcgccgg acgtgctcgt gtcctcgggg cgcatcgaca
     3121 acgcagtgga catgtattac gacctcgaga cgcaccagcc gcacaacccg gcgctcgcgt
     3181 tgaagatcgg gcggatcgcc gtgcttcgtc attcgacgga ggtcgccgag gtcgagctga
     3241 agaaggtcga gcagatggat cccaactacg gagcgcacat tctgaaggcg tacatcgccg
     3301 cgcagtcatc gaacaaaacg ctcgccgacg ccgagatgaa ggcggcttct gctgcctcga
     3361 agccgggcga tgactactgg acgactgccg gcgagatcgc ggcgatggct ggcgacacga
     3421 agggcgtcga cgatgcactc gagcacgccg ccgcgcgcaa agagccgacg gccagctacg
     3481 tgctcgccaa tcctctcttc ggattcatgc aaagtgacgc gcgctttgcc aagctgcgcg
     3541 aaacgctgaa gggacagcag aatgagattc gttccgcgct cgcaggcctg acgatataga
     3601 ttccacgctc atgatcgtca ccgccagact cacattctcc gccgcgcacc ggctgcacaa
     3661 ccccggctac gacgccgatt ggaaccgccg catctacgac aagtgcgaca acccgaaagg
     3721 tcacggccac aactacgtga tcacggtcag cgtgaaagga aagatcgatc ccgagaccgg
     3781 catggtcatc gatctgaagc ggctcaagga catcgttcga aagcagatca tcgatcgcgt
     3841 cgaccacacg aatctgaacg aagacgtcga gtttctccat ggtgtgatcc cgaccgccga
     3901 gaacctggcg cagtctttct ggaggcagtt ggcgcccgcg atcaccgagg gagcgctgta
     3961 cgaagtcgtg ctgcaggaga cggaaaagaa ctccgtcgtc tatcgcggag aggacgaatg
     4021 aactacgacc tggcgatgat ggcgctcgat ggcctgatgc ggacgccgtt gacggtgcgc
     4081 acggacagcg ccggcgtgac gttcaccggc gagccagccg ccttccgcga gctcgcccgc
     4141 ctctgcctcc tcctcggtgg cgcaagcgac gccgacgatt cgttcgagct gcaaccgaac
     4201 gtgcacgtga ccgcggattc gccgaaggtg atgttgaagt tggagacgta ggttgctggt
     4261 tgctcgctta ctgagaacat caatcaaaga tgtccggagg caggccgaag tagttgatga
     4321 tgtcgcggag atacctacgg tctatctcgg caccatcgtt gtgacacgga aaagggtaat
     4381 gcgactcgcc gtctttggtc gtcaggacaa ccattcgatg actgcctttg cccctttcga
     4441 cggatacgct gaatcgcttg tcatgcgccc gcagccgctt caacatctcg cggcaacgaa
     4501 gcaacttagg cattcgccgc gatccaagtc tctccgtgtt gcatcgctaa aagggatagt
     4561 ggaaccatgt cggcgactag atcggttggt ggctcgaagc cggagatttc cgcgacgaaa
     4621 cggtttcggc ggccctcttc aaacatgcgc caatacttgt catcagcgcg attccaaacg
     4681 ctttcggggt ggcccttctg gagagcaaaa ctcacctgag ctcctagcat ttcgacaagg
     4741 tcgtcgatcg ccttcttcac cgtcgatccg tatccacgaa ggttcatctc tagcgctagc
     4801 gctgtccagc ctgagtcctc acggaaggca attacgctga cttcgaaggt cttcggttcg
     4861 tcactcattg agtttcaccc ttagcaaccg cctgagcatc gcggattaag ttgccttgaa
     4921 caacatgggg tagtggacaa tcttcccgcg catgggaccg ccactggttc gatggttgcg
     4981 gcgcccttcc ggcggtaaag atgcggcttg cgtcccgcag tgtcaagcat tccctgaccg
     5041 cgggtggtca aggaaccgag accactccca gcctcccaac cccccgccca cccgaatttt
     5101 ctgctactct ctgcaactgc agataagtgg cggcgggtgt gatggaaatt ctgcaatttg
     5161 ccaggagcag acgggggggg gagctgatcg ggatcgtgat cctggcggcc ggtatttgtc
     5221 ttgccctcgc gcttctcacc taccacgccg acgactcctc cgcgttctac acctccacca
     5281 acagcggcgt agccaactgg atcggctact acggcgccac cgtcgcctgg atctgcgtcg
     5341 gtttcttcgg ctttgcgtcg cttctcttcc ccggtgccct gctggtcgtc ggctggaacc
     5401 gcttctgggt caaagagatc gagtacctgc agaccaaggc catcggtttc gtgatcctcg
     5461 tcgcggccat gccgccgctt tgcgacctcg cgttcggcaa agtgtgggtc cgcggcgcgc
     5521 agattccgtc cggcggctac ctcggccagg agctcaaccg cgccgcctcg gggaagctga
     5581 acagcagcgg ggcgggcatc gtccttgtca cggccatgct cgtcggactg ctgctggcca
     5641 cgcgcatcag cctcgccgcc gtcttcaggg ccatccatca gaagctcctc gggcttggcc
     5701 gcgccatctc cctgcaatgg gcgcgcttca ccgaacggcg ccgcaaagac aagatgaaga
     5761 cggcgctcgt ccgcaagcat ctcgaaaaag acgcccccgc attgcgtctc gtcgctgatg
     5821 atgagatgcc ttcctcgatg cccggcgccg gaccgatcgt gcgggaagtc aaaggcgccg
     5881 gccgattcca gatccgcaaa gtcacgaagg ccgatctccg caaagctgcc gaagagctgt
     5941 cgaaacagcc ctcgcacgat ccatcgacct tgtgggccga ggtgcagcaa caggcggaga
     6001 agccgcagtt gtcaaggccc gtcgcagttg acgacgacgg cgaattcgaa gatccgctct
     6061 acctcgacga tccgcccgcc gttcgtccgg cgcgtccgaa gcagcagatg ccgcgtcccg
     6121 ttccgcgcaa gctgaaagaa gcgccgccga agcgcgagac gcggctgacc cacgatcttc
     6181 ttccgcctgt cgatctcctc accgaaggtc cgaagcagga cacgatcaac gacgacgtcc
     6241 acaagaagtt cctcgacatc ggccgcctca tcgaagaccg atgcaaggag tttgcggtcg
     6301 aaggtgaggt caccgcctat cacccgggcc cggtcgtgac gacgttcgag ttcaagccgt
     6361 ccgccggcgt gaagtacgcc aaggtcgtga acctcggcga tgacctcgcg ctcgcgctca
     6421 aggccgaatc gatccgcatc gagcgcatca gcggatcgtc gacggtcggc atcgaagtcc
     6481 cgaacaagaa gcgcgagctc atcacgctgc gcgacatcat cgacaccgac gccttcacgc
     6541 agcagtcgtc gctcctcact ctcgccctcg gcaaggacat ccacggcgag gccatcgtca
     6601 ccgacctcgg gcgcatgccg catctgctcg tggccggtgc cacgggcgcc ggcaagtccg
     6661 tcggcctcaa ctcgatgatc gtctcgctcc tctacaaagc gctgccgaaa caactgcgga
     6721 tgctgatgat cgatccgaag atggtcgagc tgaagatgta cgaggacatc ccgcaccttc
     6781 ttcatcccat cgtcaccgat cccaagctcg cctcgaacgc gttgatctgg gccgtgaacg
     6841 agatggagaa ccggtatcgc tgcctggccg aatgcggcgt gcgcaacatc gaccaataca
     6901 acgcgctgct caaggacgtc gatggttatc ggcgcgcgat gaagcggccg gaaggggagc
     6961 tggtcaaggt ccccgagccg ctcgtttaca tcgtcatcat catcgacgag tttgccgatc
     7021 tgatgatggt ggcgtcgaag gacgtcgaag actcggtgac gcggctggcg cagaaagcgc
     7081 gcgcggtcgg cattcacctg atcatcgcca cgcagcggcc gtcggtcgac gtcatcaccg
     7141 gcgtgatcaa ggccaacttc ccgtcgcgca tcagctacca ggtggcgtcg aagatcgact
     7201 cgcgcacgat cctcgacgcg cagggcgccg agaagctcct cggcaacggc gacatgctct
     7261 tcctcccgcc cggcaccgcg cgcgtccgcc gcatccatgg cgcgtacgtg tccgagaaag
     7321 agatcgtgaa gatcgtcgac ttcgtgaagg cgaaccaggg cgagccgcag ttcctcgaag
     7381 agatcacgaa ggtggtcgac gagaagagcg gcgccgacgg catcgagtat ctcgacgatc
     7441 cgaagtacga agacgccgtg cgcgtggtgc tcacgaccgg acaggccagc gcctcctaca
     7501 tccaacgccg cctcaagctc ggctactcgc gcgccgcgcg cctgatcgag atcatggaag
     7561 ccaacggcgt cgtcggcccg tcgcagggtt cgaagccacg agagatcctg gtccgcgcgg
     7621 atgattaccc gccagctagc ggccagacga tttgattcaa aacctatcac cacagagaca
     7681 cagcgcggca tagccgcaac caaatgaaac ccaccaaatc tgcaaccaat cagttttggt
     7741 gaaaatcttt gcgtcgcgcg cagaaaccgc tggttagtag tacagag
//
LOCUS       DATLVW010000578         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_478276, whole genome shotgun sequence.
ACCESSION   DATLVW010000578 DATLVW010000000
VERSION     DATLVW010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478276"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VNN25_17070"
     CDS             complement(<1..246)
                     /locus_tag="VNN25_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93298.1"
                     /translation="MIVSLNWTQLRSWDGSQNRAFEELCCQLAHAEIFPAGSLFTRKG
                     TPDAGVECFWSLPSGEETGWQAKFFSSLDKTQWKQIDE"
     gene            complement(365..1051)
                     /locus_tag="VNN25_17075"
     CDS             complement(365..1051)
                     /locus_tag="VNN25_17075"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HXH93299.1"
                     /translation="MAGRLLPRDLAALTTIGILRMLTRTQLQAWHFETVSETVVRRFI
                     DRMFERGYLGQERINRNGVQVVWLTQAGADLLVDHAAVSRADLFPARGPVAAKDFDHT
                     TAIIDAALAVQRRGFRSDLILPAWALQRAQAGRAEVFPDLLCLARPSATNPGAVLAVE
                     VDLGGEPIGSVLVPKTEKLAAFLAPYGGSTLAILLLTVGSRRRAAMEQALRKLVISVP
                     ISIEELANFI"
     gene            complement(1054..1356)
                     /locus_tag="VNN25_17080"
     CDS             complement(1054..1356)
                     /locus_tag="VNN25_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93300.1"
                     /translation="MILFGKPMRLIALAGLYILASPVYAVLGVRKLVKLIQTRRTLAS
                     GEIACPHCGERNVLDILATCGKCKVTEYGNRLRCNACGEISKGFECDACGVTIRVL"
     gene            complement(1353..>2309)
                     /locus_tag="VNN25_17085"
     CDS             complement(1353..>2309)
                     /locus_tag="VNN25_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93301.1"
                     /translation="LLDDAFRERTLAPKLHDPALRDSIEHLDDVLPTATRQALVRQFD
                     MHLSTRLGRIWFGLSPAAIARLVPQRADARIVIGNFGPSLLLSPSLARTQAVNRLIDV
                     LTATMVNASPVATLLLIEEIGTIIRHTAVARFLLEGLRTLRWKLLSIVCCAQDPTNAI
                     PKDVLHALLLNMRWLLAFESGREDASILLPYLPPRASEAERKSAFLAEMATLPPQHCY
                     FLRKGLPPLRMKTRDLPDPKTSGKSRDELLDIFYQAIASHSMVRVVDAERLIDEEERD
                     WATGDIPPQSPRNASGPAKDTMRTVDDLFALLGRNKGTGDPP"
BASE COUNT          452 a          708 c          731 g          418 t
ORIGIN      
        1 ctcgtcgatc tgcttccact gcgtcttgtc caaggaagag aagaactttg cctgccaccc
       61 tgtctcttca ccactgggca aactccagaa gcattccacg ccagcgtcgg gcgtgccttt
      121 tcgcgtgaac agcgaacccg cagggaaaat ctccgcgtgc gcaagctggc agcaaagctc
      181 ctcgaacgcc ctgttctgag agccatccca cgaccgcagt tgagtccagt tgagcgacac
      241 gatcacaata tacaaggtcg agccctaata cgccctggtt gcaacgggca tatctcattg
      301 aaatagcagc ggttacctgt cacgaaagtg ttccctggcc cgaacacttg gaccgaacac
      361 tttctcagat gaaattggca agttcttcga tcgagattgg aaccgagatg acgagcttcc
      421 gcagcgcctg ctccatggct gcccgccgcc tactcccaac cgtgaggagc aggatcgcca
      481 gcgtcgatcc gccatacggc gcgaggaacg ccgccagctt ttccgtcttc ggaacgagca
      541 cactcccgat cggctcaccc cccagatcga cttcgacggc cagcacggct ccggggttgg
      601 tggcggacgg tcgcgcgagg cagagaagat caggaaagac ctcggcgcgg ccggcctgcg
      661 ctcgttggag tgcccacgct ggtaggatga ggtctgaccg aaaaccgcga cgttggacgg
      721 ccagagccgc atcgatgatc gcggtcgtgt ggtcgaagtc cttcgccgca accggaccgc
      781 gtgcgggaaa aagatcagcg cgcgaaaccg cagcgtgatc gacgagcagg tcggcacccg
      841 cctgcgtcag ccagaccact tgaacgccgt tgcggttgat gcgctcctgg ccgagatagc
      901 cgcgctcgaa catccgatca atgaagcgtc ggacgacggt ttctgagaca gtctcgaagt
      961 gccatgcttg gagttgcgtc cgcgtcagca tgcggaggat gccgatggtc gtcagcgcag
     1021 cgaggtcgcg aggaaggagt cggcctgcca cggctagagt acccggatcg tgacgccgca
     1081 ggcatcgcac tcgaagccct tggagatctc cccgcaggca ttgcaccgga ggcggttgcc
     1141 gtactccgtg accttgcact tcccgcaggt cgcaaggatg tcgagaacgt tcctttcccc
     1201 gcagtgcggg caagcgatct cgccactcgc gagcgttcgt cgcgtctgga tgagcttcac
     1261 cagcttgcgc acgccgagga cggcgtagac gggcgaggca aggatataga gccctgcaag
     1321 cgcgatgagg cgcatgggct ttccgaagag gatcatggtg ggtctcccgt tcccttattt
     1381 cggccgagga gtgcgaagag gtcatcaacc gtccgcatgg tgtccttggc tggtcctgat
     1441 gcgttccgtg ggctctgagg cgggatgtcg ccggtggccc aatcccgctc ctcctcatcg
     1501 atcaggcgct cggcatcaac gacgcgcacc atcgagtgcg aggcgattgc ttggtagaag
     1561 atgtcgagca actcatcgcg actcttgccc gatgtttttg ggtcgggcag atcgcgggtc
     1621 ttcatccgca gtggcggaag tcctttgcgc aggaagtagc aatgctgtgg cggcaaggtc
     1681 gccatctcgg cgaggaatgc cgacttccgc tcagcttcag atgcgcgtgg tggaagatac
     1741 gggagcagga tcgaggcatc ctcccgaccg ctctcgaagg cgagcagcca ccgcatgttg
     1801 agcagcagcg catggagcac atctttcgga atcgcgttcg tcgggtcttg cgcgcagcaa
     1861 acgatggaga gcagcttcca gcggagcgtc cgcaagcctt cgagcaggaa cctcgcgacc
     1921 gccgtgtggc ggatgatcgt gccgatttct tcgatgagga gcagcgtggc gacagggctc
     1981 gcattcacca tcgttgccgt cagcacatcg atcaggcgat tcaccgcctg cgtccgcgcg
     2041 agcgacggcg agagcaggag cgacgggccg aagttgccga tcacgatgcg cgcatccgcc
     2101 ctctgaggca cgaggcgcgc gatcgccgcc ggcgacaagc caaaccagat ccggccgagg
     2161 cgcgtgctca agtgcatgtc gaactggcgc acgagtgctt gcctcgtcgc agtgggaagc
     2221 acatcgtcga ggtgttcgat cgagtctcgc aacgctggat cgtgaagctt cggcgcgagc
     2281 gtgcgttcgc ggaacgcgtc gtccaacag
//
LOCUS       DATLVW010000579         6485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_560770, whole genome shotgun sequence.
ACCESSION   DATLVW010000579 DATLVW010000000
VERSION     DATLVW010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560770"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VNN25_17090"
     CDS             complement(<1..308)
                     /locus_tag="VNN25_17090"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HXH93302.1"
                     /translation="MSASLQQFWHYDGGIPPMHPPQLFEANLDVIERAITQVARQVRL
                     RGADAEDFASSVRIALLADDCAVLRKFEGRSSFASYVAIVVRRLFLDQKRSEGRWYPS
                     A"
     gene            complement(537..1445)
                     /locus_tag="VNN25_17095"
     CDS             complement(537..1445)
                     /locus_tag="VNN25_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93303.1"
                     /translation="MGRLTITFSGICTHFYRCIPVGAGPNTIPLRSVLPNALGVHFGM
                     VRRPETEANDPVQPMYYLMPHIAVVADTETLTPFTQSNYLYGAQLEVLNPDPLQGIFE
                     FDSSLANGYHLPDFVSPDSGIQPSRDVVQDGNAACYFDFFYGKGQVEGDTNLTTVVTI
                     ETVAIPPFDDTPRVRITPFESSLMEGPVDWTVDTNELFVSNQDFDASAEDKPFDFMLN
                     FLTTFPGIPRFLTQNVPGMTSLLTPLTPPLLSEKMATLSEAIRELGPEFPVRGKELPS
                     LVQWLASRIISQGVDLDASCSDSHFP"
     gene            complement(1676..2554)
                     /locus_tag="VNN25_17100"
     CDS             complement(1676..2554)
                     /locus_tag="VNN25_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93304.1"
                     /translation="MGTLTITFSGICTHFHDCLPWDQPGPKIPMRSVLPNAMGVYFGM
                     VQLPPPADPSVATYYLMPHVAQLSSPTADAPRRLLYGVQLEVVNVDTTQPEFTFDWSI
                     PGYHLREYVPKPEPEPSPDVVSNYKAACYFDFHFGSGKVVPSTDNPLMLTTQVQLATN
                     GPPVIRVSPFPGSPAGGDPWFIQTETLFVANQDFDADVEDKPFDFMLNYLVTQQGLPQ
                     LLTENMPGMEDGLEELTPAVLGKSMVDLGVAILNLPPPSASGQQGNSSAAALFERLPF
                     QSFVDLDQSCSDSHFP"
     gene            complement(2542..5817)
                     /locus_tag="VNN25_17105"
     CDS             complement(2542..5817)
                     /locus_tag="VNN25_17105"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH93305.1"
                     /translation="MSSPTQHLDPETLAAFAEGQLDAAGRKEVIAHLDQCEECLNDVA
                     LVMPSAGAESEKRRFLRPTWIIAIAAVVVLAIALAALRQAIHPSSPTDTLVALAPRSA
                     RLVEPRLTGGFPWAGYHGPMRATGTTPDAQQLKLGGAAGDLIERAEHDQSAEAQHAAG
                     VAMVLVEKPDEAIAKLETAARASHDAKSWSDLAAARYATASKLGRASLYPEALAAADA
                     ALRIDANLPEALFNRALILERLGLTDEAKHAWQRYLETDSSSDWATEARSRIAQLPAS
                     TQASQFEHDRPLLERAAERGDTAAVRRYVDAHRERARAYAEGIYLDNWAEALQRNDSS
                     ETARWLTIARGVGDALFAISGEALLRDAVRAVDAATPAQQRDIAAAQVLYFSGRTAYS
                     RDRRDDAERDLLGAAQAFEAAHDPMALQAHYYAASARLVGNDIAGARARLDRVMAAAN
                     AHPEYISLGGHVRWELGRAMSNDDDWLQATVVLAEGTAMFRRCGEHASEATVEMILAS
                     AYRALDRDDDAWLARLQSFAALSAEGESERLATSIAVATYAELAVGHRDAALSLSALE
                     TSIARGGARPQFAIKPLVNRALLQSMSGDTAGALQTAREAEGLARAVADPEMRAGWSA
                     QTDVATGAALAESSPGAAIEPLTRAIDFYTAHGLAANLPQPLLIRSRCSVKNGDTAAA
                     MRDLERGMTIVESHPAPIPSATAGAGVLDAEHALFTDAIRLSLDRGDKAGAFALAERS
                     HGTPATIRELQSRLIGTGTAVLEIVALPGEVVTFAVAENDAVVVRRPANLETLASLAE
                     ESLSETGTVAAVRLYDELIHPVDSVLDRAREVVIVTDQRLAAVPFAALYDSVHRTFLV
                     ERFAVSAASSAGSLQPDETRTAAPALAVISLPSGGTDSRGLPDAENEVRDVAGLYPRA
                     TTIPAAAARFAQFRTAAAGADIVHIAGHTERQPGGGEQALLFVGAAGKLERVSWKTIV
                     ASPSPNRGVVVLAACETLRPPPSTATRALSLGAAFSAAGASDVIGTLSPIGDRDARAL
                     FGALHRHLAAGARPAEALRAVQLEAITIEKSGGHRTWRAIALLTRRIPAPPYRKELLS
                     WVR"
     gene            complement(5814..>6485)
                     /locus_tag="VNN25_17110"
     CDS             complement(5814..>6485)
                     /locus_tag="VNN25_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93306.1"
                     /translation="EGRSSFASYVAIVVRRLFLDQKRSEGRWYPSAEATRQGDAAITL
                     ERLLHHDRRPLSEALSITKAQHPEADIAQLEATAASLPERAPRPRLVPVIDGDEDRFA
                     SSSSADDRILAADLNRASDRTSHAVQEALAAMTAEDRVTLRLRFSKGMAISDIARALG
                     VAQRPLYRRVESMLKDLRASLERAGIDAAEAANLVASAGDRLDFGLQSGKTDPDQPSE
                     QSETR"
BASE COUNT         1144 a         1983 c         2203 g         1155 t
ORIGIN      
        1 gccgagggat accaacgacc ctccgagcgc ttctggtcga ggaagagccg gcggaccacg
       61 atggccacgt agctggcaaa ggatgagcgt ccctcgaact tgcgaaggac tgcgcagtcg
      121 tcggcgagca gtgcgatccg cacagacgag gcgaagtcct ccgcgtccgc gccgcgcagg
      181 cggacctgac gcgcaacctg cgtgatcgca cgctcgatca catcgaggtt cgcctcgaac
      241 aactgcgggg gatgcatagg cggaatccca ccatcatagt gccagaattg ctgaagagat
      301 gcgctcatgg cgtaccgccg atgtcagcga gtgtcgtgat ctcgattcgg tccgtgatgc
      361 ggtctttccc tggccaagcc gctggtgtcg atgaggcggt cttccatggg gccaaatctc
      421 cgccggcgta atggacgcgc acacccacga ggagggttcg atcgggtgtc gatttcggcg
      481 gacggcggcc cttaggctgg ataaaccggc ctacgagcgc ggcatgatca gctccttcaa
      541 ggaaagtgac tatccgagca tgacgcgtcg agatctactc cctgcgaaat gattcgagag
      601 gcgagccact gaaccaatga aggcagttcc ttcccgcgga cggggaactc cgggcccagc
      661 tcacgtatcg cctcgctcag cgtcgccatt ttttctgaaa gcaacggcgg tgtcagtggc
      721 gttagaagac tcgtcatgcc gggaacgttt tgggtcaaga atcttggaat gccgggaaac
      781 gtagtgagga agttcagcat gaagtcaaag ggcttgtctt ccgcgctggc gtcgaagtcc
      841 tggttagaca cgaacagttc gttggtgtcg actgtccagt caaccgggcc ttccatcaga
      901 gaactttcaa aaggtgtgat ccggactctc ggagtatcat caaagggagg tatcgcaacg
      961 gtctcgattg taacgactgt cgtaagatta gtgtccccct ctacctggcc ctttccatag
     1021 aaaaagtcga agtaacacgc cgcgttgccg tcttgaacta catcacgcga cggctgaatg
     1081 ccagagtcgg gggaaacgaa gtcgggaagg tgataaccgt tggcgagcga cgaatcgaat
     1141 tcgaagattc cctggagggg gtccggattg agtacctcca actgagcgcc atagagatag
     1201 ttgctctgtg tgaagggtgt caatgtctct gtgtcggcaa caactgctat gtgcggcatg
     1261 aggtagtaca tcggttgcac aggatcgttc gcctccgtct ctggacgacg gaccatgccg
     1321 aagtggacgc ccaaggcatt tggaagcacc gaccgcagcg gaattgtgtt ggggcctgcc
     1381 ccgacgggaa tgcaccggta gaaatgtgta cagatgcctg agaaggtgat cgttagccga
     1441 cccatgacgc agctcctatc tgtgttgcgg cgcgggaatg cgcctggtca taaggcgatg
     1501 gcacactgtc ccggcggccg ccgaaggtct tctgtgtgtt ggtggcttct gcccggcgcg
     1561 gagcgcgtcg gcaggacgct caccccagcg agatggcagt gcagagcgcc gaaaagaagc
     1621 cccgcgacgc ggtcgcctac tggggtcagc gtgctcatga cgtcgcccgg ccccttcatg
     1681 gaaagtgact atccgaacac gactgatcca gatccacgaa ggattggaag ggcaaacgtt
     1741 caaacaaagc agccgcggag gagtttccct gctggccgct ggcggatgga gggggcagat
     1801 tcaggattgc tacgcccaaa tcgaccatgc ttttcccgag cactgccggc gtcagttcct
     1861 ccagaccgtc ttccatgccg ggcatatttt ccgtcaacaa ttgtggaagg ccctgttggg
     1921 tcaccagata gttgagcatg aagtcgaagg gcttgtcttc cacgtccgcg tcgaagtcct
     1981 ggttggccac gaacagggtt tccgtttgga taaaccaggg atctcccccc gcgggcgatc
     2041 cgggaaaagg gctgactctg attactgggg gaccattcgt ggccaattga acttgagtcg
     2101 tgagcatgag cgggttatct gtgctgggca ctaccttccc gcttccaaag tgaaagtcga
     2161 agtaacacgc cgctttgtaa ttggaaacta catccggcga tggctccggc tccggcttcg
     2221 gaacgtattc gcggaggtga tacccgggga tcgaccaatc gaacgtgaac tctggttgcg
     2281 tcgtgtcgac gttgaccacc tccaactgaa cgccatagag caaccgccgg ggggcgtctg
     2341 cagtgggact ggaaagctgt gccacatgcg gcattaagta gtaggtggct accgaagggt
     2401 cggccggcgg tggtagctgc accatgccga agtagacgcc cattgcattg ggaagcaccg
     2461 accgcatcgg aatctttggg ccgggttgat cccatgggag gcagtcgtgg aaatgcgtgc
     2521 agataccgga gaaggtgatg gtcagcgtac ccatgacagc agctcctttc tgtatggcgg
     2581 cgcagggatg cgcctggtca atagagcgat ggcacgccat gtccggtgac cgccgctctt
     2641 ctcgatggtg atcgcttcca gctggacggc gcggagcgcc tcggcagggc gtgctcctgc
     2701 cgccagatgg cgatgcagtg cgccaaagag agcccgcgcg tcgcggtcgc cgatcgggct
     2761 tagcgtgcca atcacgtcgc tcgctcccgc ggccgagaat gcagcgccaa ggctgagcgc
     2821 acgcgtcgcg gtggagggag ggggacgaag cgtctcgcac gcggcaagca ccacgactcc
     2881 acgattcggc gagggcgaag cgacgatggt cttccatgag acgcgctcga gctttcctgc
     2941 tgcgccgacg aacaacaacg cctgttcgcc gccgcccggc tggcgctcgg tgtggccggc
     3001 gatatgtacg atgtctgccc ctgcggcggc cgttcggaac tgcgcgaacc tggcagcggc
     3061 ggcagggatc gtcgtcgcac gcgggtagag cccggcaacg tcgcgcacct cattctccgc
     3121 gtccggcagc cccctcgaat ccgtgccgcc gctcggcaga gaaatgacgg cgagcgcggg
     3181 agctgcagtt cgcgtttcat cgggctgcag cgagcctgcg ctcgaggcgg ccgacaccgc
     3241 aaatcgttca acgaggaatg tgcgatggac ggagtcatag agcgccgcga acggcacggc
     3301 cgccagccgc tggtccgtga cgatcaccac ctcgcgcgcg cggtcgagga cgctatcgac
     3361 tggatgaatc aattcatcat agagcctgac cgcggcaacg gtccccgttt cggaaaggct
     3421 ctcctctgcg agcgacgcga gcgtctcgag gttggccggg cggcggacga cgactgcgtc
     3481 gttctcagcg acggcgaacg ttaccacctc gccgggcaga gccacgatct ccagcaccgc
     3541 agtaccggtg ccaatgaggc ggctctgcaa ctcgcgaatg gtggccggcg ttccgtgcga
     3601 tcgctcggca agcgcgaatg cgcccgcctt gtcgccgcgg tcgaggctga gacggatggc
     3661 gtcggtgaag agggcgtgct ccgcgtcaag aacgcctgct ccggcggtgg cactcggtat
     3721 cggcgccgga tggctctcca caatagtcat tccgcgctcg agatcgcgca tcgccgccgc
     3781 cgtgtctccg ttcttaacgc tgcagcgcga gcggatgaga agcggttggg gcaggttggc
     3841 ggcgagtccg tgtgcggtgt aaaaatcgat cgcccgcgtc agcggctcga ttgccgcacc
     3901 agggctcgac tccgccagcg ccgcccccgt cgcgacgtcc gtctgggcgc tccaaccggc
     3961 ccgcatctca ggatcggcaa cggccctggc cagtccttcg gcctcgcgcg ccgtctgcag
     4021 ggcgccggca gtgtcgccgc tcatcgactg cagcagcgcc cggttgacga gcggcttgat
     4081 cgcgaactgc ggccgcgctc cgccgcgcgc aatggaggtt tccagcgccg acagcgagag
     4141 cgcggcgtcg cggtgaccga cggcgagctc ggcgtacgtc gccaccgcaa tgctcgtggc
     4201 cagacgctcc gactcgcctt ccgcgctcag cgccgcgaac gactgcaagc gcgcgagcca
     4261 cgcgtcgtca tcgcgatcga gagcgcggta ggcagatgcg aggatcatct cgacggtcgc
     4321 ttcgctggcg tgctcgccgc accggcggaa catcgccgtt ccttcggcca gcaccactgt
     4381 cgcctgcagc cagtcgtcat cgttgctcat cgcgcgcccg agctcccacc gcacgtggcc
     4441 tccaaggctg atgtactcgg gatgcgcatt ggctgcagcc attactcgat cgagacgggc
     4501 gcgtgctccg gcgatgtcgt tccccaccaa ccgcgcgctg gcggcgtagt agtgggcctg
     4561 aagagccatc ggatcgtgcg ccgcctcgaa cgcctgggcg gcgcctagca gatcgcgctc
     4621 cgcatcgtcg cgcctatcgc ggctgtaggc ggtacggcca gagaaataga ggacttgcgc
     4681 tgcggcgata tcgcgttgct gcgcgggcgt ggccgcgtcg acggcgcgca cggcgtcgcg
     4741 cagcagcgcc tcaccggaaa tggcgaatag cgcgtcgccg acgccgcgcg caatggttag
     4801 ccatcgcgct gtctcggagc tgtcgtttcg ttgcagcgct tccgcccaat tgtcgaggta
     4861 gatgccctcg gcgtaagcgc gggcgcgctc ccgatgtgcg tcgacgtacc ggcgcaccgc
     4921 ggcggtgtcg ccgcgctcgg cggcgcgctc gaggagtggg cggtcgtgct cgaactgcga
     4981 tgcctgcgtt gaagccggca attgcgcgat gcgcgaccgc gcctcggtgg cccagtctga
     5041 cgaagagtcg gtctcgaggt aacgctgcca ggcgtgcttc gcttcgtccg tcaagccgag
     5101 gcgctcgagg atgagtgcgc gattgaagag cgcctcgggc aggttcgcat cgatgcgaag
     5161 cgcggcgtcg gccgcggcga gcgcctcggg atagagcgac gcgcgcccga gctttgatgc
     5221 ggtggcgtag cgcgccgcgg cgagatcgct ccacgacttc gcgtcgtgcg aggcgcgggc
     5281 ggcggtctcc agtttggcga tcgcttcgtc cggtttttcg accagcacca tcgcgacgcc
     5341 ggcggcgtgc tgtgcctcgg cgctctgatc gtgctcggcg cgctcgatca gatctccggc
     5401 ggcgccgccc agtttgagtt gctgcgcgtc tggagtggtg ccggtggcac gcatcgggcc
     5461 gtgatagccg gcccagggga atccgccggt caggcgcggc tcgaccagac gcgccgagcg
     5521 tggggccagc gctacaaggg tatccgtcgg ggagcttgga tgaatggcct gccgcagcgc
     5581 agcaagcgcg atggctagaa cgacgacggc ggcgatggca atgatccagg tcgggcggag
     5641 gaagcggcgc ttctccgatt cggcgccggc cgagggcatc acgagtgcga cgtcgttcag
     5701 gcactcctcg cattgatcga gatgtgcgat cacctctttg cgacctgccg catcgagctg
     5761 cccttccgca aatgcggcga gggtttcggg atcgaggtgc tgggtcgggc tgctcatcgc
     5821 gtctccgatt gttcggacgg ctgatcgggg tccgttttcc ccgattgcag gccgaaatcg
     5881 agccgatcgc cggcggaggc gacgagattg gccgcttccg cggcgtcgat tccggcgcgc
     5941 tcgagtgacg cgcggagatc cttgagcatc gactcgacgc ggcgatagag cgggcgctgc
     6001 gccactccga gggcgcgggc aatatcggag atggccatgc ctttggagaa ccggaggcga
     6061 agggtgacgc ggtcctctgc cgtcatcgcg gcgagcgctt cctggacggc gtgagaagtc
     6121 cgatccgagg cgcggttgag atcggcggcc aggatgcggt catcggcgga cgacgagctc
     6181 gcgaaccgat cctcgtcgcc atcgatgacg ggcacgagcc ggggacgcgg tgcgcgctcg
     6241 gggagcgacg ctgcggtggc ttcgagctgt gcgatgtcgg cctcggggtg ctgcgctttc
     6301 gtgatgctca acgcttcgct gagcggccga cgatcgtgat gaagcaggcg ctcgagggtg
     6361 attgcggcgt caccctgccg cgtcgcttcc gccgagggat accaacgacc ctccgagcgc
     6421 ttctggtcga ggaagagccg gcggaccacg atggccacgt agctggcaaa ggatgagcgt
     6481 ccctc
//
LOCUS       DATLVW010000580         3266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_234651, whole genome shotgun sequence.
ACCESSION   DATLVW010000580 DATLVW010000000
VERSION     DATLVW010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234651"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VNN25_17115"
     CDS             <1..313
                     /locus_tag="VNN25_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93307.1"
                     /translation="RLGSVAFLDSDEVDDFLNALNFIISCAADMAVRARDYTEVTFST
                     RDNLTVGFLQDGQTQQAIARLSAGHQFLGFRVTQLHTIRNSVIQANEYVSTRRAAWEP
                     R"
     gene            complement(391..825)
                     /locus_tag="VNN25_17120"
     CDS             complement(391..825)
                     /locus_tag="VNN25_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93308.1"
                     /translation="MNRALVIVSLATFAAGCRAGSQPAKAQPQTKAEMRTIDSGNVQP
                     ATIDAPPVLTDEAPPLKTATNLAQADAPPPPTPQDEALRAALPFAPAIAMDAVDGSKI
                     SIRASTPTTEYKNKLYYFSSEDHKRTFLANPEQYTKGLFSHL"
     gene            complement(866..1279)
                     /locus_tag="VNN25_17125"
     CDS             complement(866..1279)
                     /locus_tag="VNN25_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93309.1"
                     /translation="MTSPSTIRCGALRSVLFADILNGVLESTHGAIGAVFLDSEGESV
                     EVVTARPFETDDHDLRVIGAYAGIFMSQLRRVASTTEGGDVARFKFDFAASRIFCCNL
                     HEGYYLVLIVDAGAVEGIVWKRLDECRTALLREMS"
     gene            complement(1239..2981)
                     /locus_tag="VNN25_17130"
     CDS             complement(1239..2981)
                     /locus_tag="VNN25_17130"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXH93310.1"
                     /translation="MSEVEPTAPPARRARLLWTLLGAMIIVGLVPLVVSHYFLISINR
                     DSLETLEKKYLTRSAVGIATDIQNLLANNTQQLTQIGGSVRAMKGALPAGSDPFIYGE
                     QRGTITNYLTPDGDLLGLRILNREGKGASATPARLDPSVRQELDLALQAALTGQVYTG
                     TFQFVTAANQPAVIIAVPVIDEGATIGTVEAIVSLRRISERIRDEGKGDVTAFLVDRN
                     GRVLMHSEASVEVQHPDFSNLKIVQEFSKAPVHLTIPYDDNRTGERVAMLGTVAPVGH
                     PDWGVIVQKPERLAYASVSKMVRATITWISIALLVAILAAIVFAAGIAGPVRALAQRS
                     REIANGNYHQRIELKTRNEIGELADNFNSMSGAIETAIEQLKKAAHENHLLFINSVRM
                     LAAAIDAKDPYTRGHSERVARYSVAIGKNLSLGEQEMRNLRISALLHDVGKIGIDDRI
                     LRKPGALSEDEFEVMKGHPAKGAAIMSGVAQLIDIVPGMKYHHEKWSGGGYPDGLTAE
                     EIPMQARIVAIADTFDAMTTNRPYQKAMEIHYVVEKIRSFAGTRFDPRVVEAFANAVQ
                     RGDITIDDQVRGAA"
BASE COUNT          600 a         1005 c         1017 g          644 t
ORIGIN      
        1 ccgcctgggg tcggtggcgt ttctcgactc cgatgaggtt gacgattttc tgaacgcatt
       61 gaacttcata atctcttgcg ccgcagacat ggctgttcgt gcccgagact acacggaagt
      121 caccttttca actcgagaca acctcaccgt agggttcctt caagatggtc agacgcaaca
      181 ggcgatagca cggctcagtg ccggtcatca gtttctgggc tttcgcgtca cacagcttca
      241 cacgattcgc aattctgtca ttcaggcgaa cgaatacgta agcactcgcc gcgcagcgtg
      301 ggaaccgcgg tagatcggct atcgttcaac cagctctcca tcagcgctct ctcccacacc
      361 ccacccccga caccccaccc ccgcaaaacc tcacaagtgc gaaaacaacc ccttcgtgta
      421 ctgctccggg ttcgccagga acgttctttt gtgatcctcg gacgagaagt agtagagctt
      481 gttcttgtat tcggtggtcg gcgtggaggc gcggatcgag atcttgctgc cgtcgacggc
      541 atccatggcg atggcagggg cgaacgggag ggccgcgcgg agcgcttcgt cctgaggcgt
      601 tggcggcggt ggagcgtcgg cctgggcgag attggtcgca gtcttcagcg gcggcgcctc
      661 gtcggtgagc accggcggtg cgtcgatcgt ggccggctgg acgttgcctg aatcgatcgt
      721 gcgcatctct gccttcgtct gcggttgcgc cttcgcaggt tgcgagccgg cgcggcagcc
      781 cgcagcgaac gtggcaagag aaacgatcac gagagcgcgg ttcaacgtga cttccggtct
      841 gtgcaggcag caagccaacg aatcgtcatg acatctcccg cagcaacgcc gtccggcact
      901 catccagccg cttccagacg atcccctcga ccgcaccggc atcgacgatc agcaccaggt
      961 agtagccctc atgcagattg cagcagaaga tcctgctggc cgcgaaatcg aacttgaagc
     1021 gcgccacgtc cccgccctcg gtcgtgcttg cgacgcgacg aagctgcgac atgaagatgc
     1081 cggcataggc gccgatgacg cgaagatcgt gatcgtcggt ctcgaatggc cgcgccgtca
     1141 ccacctcgac gctctccccc tcggagtcga gaaacaccgc gccgatcgca ccgtgtgtcg
     1201 attccaggac gccgttcagg atgtccgcga acagcacgct acgcagcgcc ccgcacctga
     1261 tcgtcgatgg tgatgtcacc gcgctggacc gcgttcgcga aggcttccac gacgcgcgga
     1321 tcgaagcgtg ttccggcaaa gctgcggatc ttctcgacga cgtagtggat ctccatcgcc
     1381 ttctgatacg gccggttcgt ggtcatggcg tcgaaggtat cagcaatggc gacgatgcgc
     1441 gcctgcatcg ggatctcttc ggccgtgagg ccgtcggggt atccgccgcc gctccatttc
     1501 tcgtggtgat acttcatgcc gggaacaatg tcgatgagct gggccacgcc gctcatgatc
     1561 gcagcgcctt tggcgggatg tcctttcatc acttcgaact cgtcttcact cagcgcgcca
     1621 ggtttgcgga ggatgcggtc gtcgatgccg atcttgccga cgtcgtgaag gagcgcgctg
     1681 atgcggaggt tgcgcatctc ctgctctccg aggctcagat ttttcccgat cgccaccgag
     1741 tagcgcgcca cgcgctccga gtgtcctcgg gtgtacggat ccttggcatc gatggccgcc
     1801 gcgagcatgc gcacggaatt gatgaagagc agatgattct cgtgcgccgc tttcttcagt
     1861 tgctcgatcg ccgtctcgat cgcgcccgac atcgaattga aattgtcggc cagctcgccg
     1921 atttcgttgc gcgtcttcaa ctcgatgcgc tggtggtagt tgccgttggc gatctcgcgc
     1981 gagcgctgcg ccagcgcgcg cacgggtccg gcgatgccgg cggcgaaaac gatcgcggca
     2041 agaatcgcaa ccagcagcgc gatcgagatc catgtgatcg tcgcccgcac catcttcgag
     2101 accgacgcgt aggcgagccg ctccggtttc tgcacgatga ctccccagtc gggatggccg
     2161 accggcgcca cggtgccgag catggccacg cgctcaccgg tgcgattgtc gtcgtaggga
     2221 atggtcagat ggacgggagc tttcgagaac tcctgcacga tcttcagatt ggaaaagtcg
     2281 ggatgctgca cttcgaccga cgcctcgctg tgcatcagga cgcgtccgtt ccggtcgacg
     2341 agaaatgcgg tcacgtcgcc tttcccttcg tcgcggatcc gctcgctgat ccggcgaagg
     2401 ctgacgatcg cctcgacggt cccgatggtc gcaccttcgt cgatgaccgg cacggcaatg
     2461 atgaccgcgg gttgattcgc cgccgtcacg aactgaaacg tgccggtgta aacctgtccc
     2521 gtgagcgcag cctgcagcgc cagatcgagc tcctggcgca ccgagggatc gagacgcgcg
     2581 ggcgtggccg aagctccctt tccttcgcgg ttgagaatgc gcaggccgag gaggtcgccg
     2641 tccggggtga ggtagttggt gatcgtgccg cgctgctcgc cgtagatgaa gggatcggag
     2701 cccgccggca acgccccctt catcgcccgc acgctcccgc cgatctgcgt cagttgctgc
     2761 gtgttgttgg cgagcagatt ctggatgtcg gtagcgatgc cgaccgccga gcgcgtcaga
     2821 tacttcttct cgagcgtctc cagcgaatcg cggttgatgc tgatgaggaa gtagtgcgag
     2881 accacgagcg gcaccaaccc gacaatgatc atcgcgccaa gcagcgtcca aagcaggcgg
     2941 gcgcggcggg cgggcggagc ggtgggttct acctcggaca tcgagcttga ttatagctag
     3001 gggccgcaca cccctgttct cagatgtgcg tgtcgggcta acacgtttgc ttcggttggg
     3061 gtggaatcaa tcctgcgata tcattgctat caaagaaaga ggaacgatat ggcccagcta
     3121 atcgtgcgaa acatcgaaga cgagctcgta caagaactga aagtaagagc agcgcggaac
     3181 gggcaatctg ctgaggagga gcaccggcaa attctgcggc gcgctctcgg gtcggagtat
     3241 tccggcttat cgatcaagga gatgct
//
LOCUS       DATLVW010000581        13010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_314837, whole genome shotgun sequence.
ACCESSION   DATLVW010000581 DATLVW010000000
VERSION     DATLVW010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314837"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VNN25_17135"
     CDS             <1..910
                     /locus_tag="VNN25_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93311.1"
                     /translation="GIRSRMKTRYGKETYYYGNFVDQLKPEKGWYTFDNRPRFGNNYA
                     GLRNRFAILSEAYSYIDFRARVEVTQQFLNEILASVARHGGTMMKIAARADQRTSLAK
                     VKRLGTRFEIKPWRKSTTILWERSTRAKPGEGIQNPETGSGPRIVRTGKIAPMKITDY
                     GIFAPTEKSEVPYAYLIDARAKHVIENLRTHGIVVERLNALSKLRVEQFTITKADHAP
                     REFQKHRELTLTGDWKSNDVSFDAGTYVVRMNQPLARLAFYLLEPRSDDGLFDWNFFD
                     ELLGDMAPVSRLMTVTPLNAGVGGGE"
     gene            1026..1946
                     /gene="argF"
                     /locus_tag="VNN25_17140"
     CDS             1026..1946
                     /gene="argF"
                     /locus_tag="VNN25_17140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXH93312.1"
                     /translation="MNHKDFIEIHDYSAAEVREIFDVAKDMKANPAKYHDALKGQTLA
                     MIFEKSSTRTRVSFEVGMFQLGGHALFLSSRDIQIGRGEPIYDTAKVLSRFVSGIMAR
                     TFAHKTVTELAEYSSVPVINGLTDLSHPCQAMTDYFTAWEHLGDLKGRKIAYIGDGNN
                     MAHSLMFGASKVGMNIAVATPGGYAPDPMIVAQAQGDADNAGTKVIVTNSIEEAVRDA
                     DVVTTDVWASMGQEAEAAKRQKDFEGWQVNNRVMSFAKKEAIFMHCLPAHRGEEVASE
                     VIDSERSVIYDEAENRLHVQKAIMFVLMKK"
     gene            2044..3051
                     /locus_tag="VNN25_17145"
     CDS             2044..3051
                     /locus_tag="VNN25_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH93313.1"
                     /translation="MPDSQTRHTRGLTVVIILGVIFFALLALLIVRSGKRPEAPPAAS
                     ETLSVLSLRARLRTQPTASAPVVATAANGEKLIVLEDRGAWVRVQDDDGMTGWAERNS
                     LERTSERARRLARYETIRKLPALNAIVSGRTPLYAGPGIFYPIIGELPADTQVKVYTR
                     DHDFYAIDHDNQIAYADVDAIDVSSAGTRQVDVHTDTAATDTAAATATSEPAVGATAT
                     QPAEPPVQTAPQVPVTADNTGVYTAVPAGGTQPEETDRVVPRFPMMARRAGIQGPVVV
                     RGIVRKDGTIDNVEVIKDLPMGLGDAAREAVSQWHFRPATYRGDPIDVYYTVTVNFRL
                     Q"
     gene            3085..3957
                     /locus_tag="VNN25_17150"
     CDS             3085..3957
                     /locus_tag="VNN25_17150"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXH93314.1"
                     /translation="MSRLTFPDTHGDLLGAHISTKGGLHTVFERAGEINASAIALFAK
                     NSNQWKGKTMSDDDVALFADKRNLRPILTHASYLINLATTNAEFHRKSIDAMIDELDR
                     AERLGIHAVVLHPGAHLGAGADAGVEQIARSLDQIHAAIPNHKVVTLLETAAGQGSCL
                     GCTFDELGQIRARVDDKARVGICFDTCHVFASGYELRNRDDYERTIDSLVEHVGIDNV
                     GAFHLNDSKKPLGSRVDRHENIGDGEIGVDAFAFLLNDKRFRGIPKVIETPKTVEHES
                     DRKNLTLLRSLLSR"
     gene            3970..5205
                     /gene="icd"
                     /locus_tag="VNN25_17155"
     CDS             3970..5205
                     /gene="icd"
                     /locus_tag="VNN25_17155"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HXH93315.1"
                     /translation="MADRISKPQSGTRIDYKDGKLLVPDDPIVPFIEGDGTGADIWRA
                     TRYVLDGAVEKIYGGKKKFHWHEVFAGDKASTQFGEYLPQETLDAIAHYRVSIKGPLS
                     TPVGGGIRSLNVTLRQVLDLYACVRPVRYFEGVPSPVKEPGKVDMVIFRENTEDVYAG
                     IEWPAESEGAKSIIEFVNGIERRGGAKIREGSAIGIKPVSEFGSKRLIRKAIEYAIAA
                     KRDSVTIVHKGNIMKFTEGAFKDWGYQLAREEFGDVTVPESEVGPEGAGGKIVIKDRI
                     ADSMFQQVLLRPSEYSVIATTNLNGDYLSDALAAQVGGLGMAPGSNESDEVAIFEATH
                     GTAPKYAGKDMINPGSLILSGVMMLRHMQWWDAADAIERAIAATIAQKKVTYDLERQM
                     TGATKVSTSEFGRVIVDNI"
     gene            5221..6858
                     /locus_tag="VNN25_17160"
     CDS             5221..6858
                     /locus_tag="VNN25_17160"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH93316.1"
                     /translation="MKRLLLTAVVATFPLMAAAQTDAPDLIIHHGKVFTADPAHRWAE
                     AVAIRGNRIVAVGTNAEITALGQDSTKRVDAGGRVVVPGFNDAHTHQGPKPEGFGLSL
                     ESDPTWALTSAALVNSAEETAGDLWIFGTIGPKLLADPSVTAQALDKAAGGRKVVLSS
                     WTGHGLIFSTAAMDALRIRDTATDPPGGWFERDAKQHVTGKMFEYAGWNAERRLADSV
                     ADADAIEQLKTFSDQSLAFGITSVQNMSMLPLARYEKIERHGPAAIRIRMIRFPMTEN
                     GSVTEGTNLSATDRERPLSIISGTKWILDGTPVERGAAIRRPYPGGGEAVGKLDFPPD
                     DIKAILKEAFESNEQTLLHVAGDRTAAAVFDAMRAIGPAEEWRKKRLRFEHGDGLEPD
                     LLPLAKDFGIVVVLNPSHIVAHSLYPAGGYMPFKSLLKAGIPIAIGSDGPANPGLNIM
                     LATTLPGNPAEALSREEAVEAYTRGSAYAEMMENDKGTLAPGKLADLAILSQDIFRAS
                     ADRLPDTESVMTIIDGKVALDNGTLKREGFPMASRTR"
     gene            6864..8201
                     /locus_tag="VNN25_17165"
     CDS             6864..8201
                     /locus_tag="VNN25_17165"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ascorbate-dependent monooxygenase"
                     /protein_id="HXH93317.1"
                     /translation="MFGPSVRRILIASAFGLALIAARQRVVLHPAPPKITNGPTFSNE
                     VVRVFQDRCQSCHHPGDIAPFSLMTYADAAPHADAIKYMTQTRQMPPWKPTPACGDFA
                     DARVLTQDEIDLLAKWADNGAPEGNVADLPPAKNFDGGWALGQPDVVLSYPEPYTPPL
                     TSDMYRCFPMPTNLPADSYISAIDIKPGDRQTVHHVIAYIDTTGDSQKLDEADPGPGY
                     TSFGGPGFSLNSLSSTLGGWAPGARPAVLPDDVAMSLPANSRIVLQVHYHPHGVAAVP
                     DQTQIGLYFAKKKPNKIVYMLPLINQNFTIPPNNPNYLVSASYNVALPVHLYLVFPHM
                     HLLGRKMHVTATPFGAAEQCLINIDDWDFNWQGQYKFSQPIAVTAGTRLSLEAYYDNS
                     TDNWRNPNNPPKPVSWGEQTTDEMCISFFGFTIDSLFATDQPPSATQIFRAVQ"
     gene            8295..10445
                     /locus_tag="VNN25_17170"
     CDS             8295..10445
                     /locus_tag="VNN25_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93318.1"
                     /translation="MTSDRESVLRFDLAVQKSHPLVYTAAFFAALAAQALGVFVLNLP
                     VAIAILLIELAVTASLYALLRRGVDRRILNPVWIVTDIVLVTLGVYASGGIASPWFIW
                     YLGTAASTAFVLGKRASYLVSLANVVAYVAVLLLMGQAAFVNSVLLLAVVRMLFLFGA
                     SFFFLAGIANLLQKRLQIRELEAEQAREVAELTRLTQELQRRSAELEDASLRVQETDR
                     LKSQFLANMSHELRTPMNSIIGFSEILIERLGDKIEEKHLSFLRHILTSGQHLLGIIN
                     DILDLSKIEAGRMEIYAEKFEVRQVIESVCAVIRGMGRTKVPVFTIDIDPRLPIIETD
                     LTKFKQILFNLLSNAVKFSPGHAPIAISAFHSGNSHDEGTITVSVRDEGVGIDPKNHE
                     VIFDEFRQIDGSERREFGGTGLGLALVKKFVQLQGGWVRVESSPGKGSTFSFTLPVRS
                     RAAVVSGTQELASAESRTEPRAERVLVVEDDAQAYALISSALGSAGYLSIRARHGEEA
                     LNLAREMRPVAVTLDLVLPGLDGWDVLKTLKSDAATCDIPVVIISRVDERDLGVALGA
                     NDYFVKPVDRDRLLDRVRQLTGSSQSKARLLLIDDDVSLHQLLEEELTTLGYTIESAF
                     TGETGFAAAKAKTPDVIILDLMMPGMSGFEVAGLLKDDPSTAKIPILVLTSKEISADD
                     RRDLQTKVAAYVQKGGSARDHLVAEIRRLQRGNA"
     gene            complement(10447..11565)
                     /locus_tag="VNN25_17175"
     CDS             complement(10447..11565)
                     /locus_tag="VNN25_17175"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HXH93319.1"
                     /translation="MPNVARSVGVMRWVALLLLLPIFVILTPATAAENAFLPALPGFD
                     FSFPRDHGAHDEYKTEWWYYTGHLETEAGKRYGFELTFFRVGIVPPGNPTATRWDLRN
                     LSLAHFAISDIGAGEFRYYEKLNRSSPFTAGTAAGVLDVFNEGWRATTLADGSWRIAA
                     YGGKDAIDLVMTSRKAPAIHGENGISIKAEGVGYASHYYSMSRLEVRGRVNGGSCHGL
                     AWMDHEFGSSQLRESQQGWDWFSVQLDNDAELMLYQIRRKDGTPDAPSSGSLISSEGN
                     VIHLRRDQMVITPLGSWHSNKSGATYPMGWRVAIPSFGIALVLQPLMKDQELVTRSST
                     QVTYWEGAVDVAGSFGNVAINGQGYVEMTGYEKAFRSP"
     gene            11591..>13010
                     /locus_tag="VNN25_17180"
     CDS             11591..>13010
                     /locus_tag="VNN25_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXH93320.1"
                     /translation="MAEQPVTMPPRARFRTLAAETSIPISADELIALARRYADQGMYD
                     EAIHLYEMAEKLKPGSVAVRINLARTRDQKEKAEASRYEAVRHEVDSQRARDEIDASQ
                     YVGLAQYYMAKDQTSKAIELLEIAKLKTPNNYRPYEILGRLHYSQGEWDSAHEEIVKA
                     RKLNPFDRGLAEISGRVEFEKRNYERALEDFIDAFLLSTDQKGESTEPVRRMINTLKR
                     IETIETKDLNARIKLRVEQLQNCTERLELRKDNLFKLESKKSLREIVDKISRDAETRD
                     NLITMAADLRGLAVFQHIKDEHLIRLSKFARVEHLAENGLVFKEDDRSMDFYVVKDGR
                     IEIRKDTPFGPQILGSLTAGVIFGEMNFIDRTHRSSDAVAVIPSSCYTFSFSALDQLM
                     DEDKQLAVGLHWAFWRSLTEKVRDANEQLKLFFQEDAKKGQGRKRAIEGKREIQQITV
                     KSEDKVDLFKERGLSAGEMKLLA"
BASE COUNT         2669 a         4154 c         3876 g         2311 t
ORIGIN      
        1 tggaattcgc tcgcggatga agacgcggta cggaaaagag acgtactact acggcaactt
       61 cgtcgaccag ctcaaaccgg agaagggttg gtacacgttc gataaccggc cccgcttcgg
      121 caacaactac gcggggctgc gcaaccgctt cgcgattctg tccgaggcgt atagctatat
      181 cgactttcgg gcgcgcgtcg aagtgacgca gcaattcctg aacgagatcc tcgcctcggt
      241 cgcgcggcac ggcggcacga tgatgaagat cgcggcacgc gccgatcagc gcaccagtct
      301 cgccaaagtg aagagactcg gtactcgctt cgagatcaag ccgtggcgca agagcacgac
      361 gattctgtgg gagcgttcaa cgcgcgccaa accgggagaa ggaatccaga acccggagac
      421 aggaagcggc ccgcgcatcg tccgcaccgg caagatcgct ccgatgaaga tcaccgacta
      481 cggcatcttc gcgcctacgg aaaagtctga ggtgccatac gcgtacctga tcgatgcacg
      541 tgccaaacac gtcatcgaga atctccggac gcacgggatc gtcgtcgaac ggctgaacgc
      601 actgtcaaaa ctgcgcgtcg agcaattcac catcacgaaa gccgatcacg ccccacgcga
      661 gttccaaaag caccgcgagc tgacgctcac cggcgattgg aagagcaacg acgtctcatt
      721 cgacgccggc acctacgtcg tgcgcatgaa ccaaccgctg gcccgtctcg ccttctacct
      781 gctcgagccg cgcagcgacg acggcctctt cgactggaac ttcttcgatg agttgctcgg
      841 cgatatggcg ccggtgtcga ggttgatgac tgtgacgcct ttgaatgcgg gggtgggggg
      901 tggggagtag ggagtgggag cgaaccggtt tgttgggtgt cggcgaacaa cacgaagttg
      961 tggtcggcgc cctctccgac tcccgacccc cgaataccga cacccgcgca tacaatcccc
     1021 accccatgaa ccacaaagac ttcatcgaga tccatgacta cagcgcggcc gaggttcggg
     1081 agatcttcga cgtcgcgaag gatatgaagg ccaatccggc caaataccac gacgcgctga
     1141 agggccagac gctggcgatg atcttcgaga aatcgtcgac gcgaacgcgt gtctccttcg
     1201 aagtggggat gttccagctc ggcggccacg ccctctttct ttcgtcgcgc gatatccaga
     1261 tcggccgcgg tgagccgatc tacgacacgg ccaaagtcct ctcgcgattc gtcagcggca
     1321 tcatggcgcg gacgttcgcg cacaagacgg tcaccgagtt ggccgagtat tcgagcgttc
     1381 cggtcatcaa cggtctcacc gatctctccc acccctgcca ggccatgacg gattacttca
     1441 cggcctggga gcacctcggc gatctcaagg gccgcaagat tgcgtacatc ggagacggca
     1501 acaacatggc gcactcgctc atgttcggtg cgtcgaaagt cgggatgaac atcgctgtgg
     1561 ccacgccagg cggctacgca cctgacccaa tgatagtcgc ccaggcgcag ggcgacgccg
     1621 acaacgccgg cacgaaagtc atcgtcacaa actcgatcga agaagccgtg cgtgatgccg
     1681 acgtcgtcac caccgacgtc tgggcgtcga tgggacagga agcggaagct gcaaaacggc
     1741 agaaagactt cgaaggctgg caggtcaaca accgcgtgat gtcgttcgca aaaaaggagg
     1801 caatcttcat gcactgcctc cccgcgcacc gcggcgaaga ggtggcatcc gaggtgatcg
     1861 actcggaacg gtcggtaatc tatgacgaag cggagaaccg cctgcacgtg cagaaagcga
     1921 tcatgtttgt gttgatgaag aagtgattga ggccgagatc ggagacaaga gatcagagat
     1981 cagggttgtt ctgcaaaatt gccctgatct ctgatctctg attttcctga tctctgctct
     2041 cttatgcccg actcccaaac ccgtcacacg cgcggcctca cggtcgtcat catcctcggc
     2101 gtgatctttt tcgcgctgct ggcgctgctc atcgtgcgca gcggcaagcg tcctgaggcg
     2161 cctccagcgg cctcggagac gctgtcggtg ctgtcgttgc gggcacgcct tcgcacgcag
     2221 ccgacagcga gcgcgccggt ggtcgcgacc gccgcgaacg gcgagaagct catcgtgctc
     2281 gaagaccgcg gcgcgtgggt tcgcgtgcag gacgatgacg gcatgaccgg ctgggctgag
     2341 cgcaattcgc tcgagcgcac atcggagcga gcccgccgtc tggcgcgata cgaaaccatc
     2401 cgcaaactgc ccgcgctgaa cgctatcgtc agcggacgaa cgccgctcta cgccggcccc
     2461 ggaatcttct atccgatcat cggtgagtta cccgcggaca cacaggtcaa ggtctacacg
     2521 cgcgatcacg acttctacgc catcgatcac gacaaccaga tcgcatacgc cgacgtcgac
     2581 gcgatcgacg tcagctctgc cggaacgcgg caggtggacg tacacaccga tacggccgcc
     2641 accgacaccg cggcggcgac ggccacatcc gaacctgctg tcggtgcgac ggcgacgcag
     2701 ccggccgaac cgccggtcca gaccgcgccg caggtgcctg tgacggctga caataccggc
     2761 gtctacaccg cggtgcctgc cgggggaacg cagccggaag agaccgaccg cgtcgtgccg
     2821 cgctttccga tgatggcccg ccgcgccgga atccaagggc cggtggtcgt gcgcggcatc
     2881 gttcgcaaag atgggacgat cgataacgtt gaagtgatca aggatctgcc gatgggactc
     2941 ggcgacgcgg cacgcgaagc ggtgagccaa tggcatttcc ggccggccac gtatcggggc
     3001 gatccgatcg atgtctatta cacggttacg gtgaatttcc gcctgcagtg acgcgttctg
     3061 cggcgcgtct acgcacccgg ctccatgtcc cgcttaacat tccctgacac tcacggcgat
     3121 ctactcggcg ctcacatttc gacgaaaggc ggtctgcaca ccgtcttcga gcgcgcaggc
     3181 gagatcaacg cctccgcgat cgcactcttc gcgaagaact cgaatcagtg gaagggaaag
     3241 acgatgtcgg atgacgacgt cgcgctcttc gcggataaac gcaacctgcg cccgatcctg
     3301 acgcacgcgt cgtatctaat caatctggcc accacgaacg cggagtttca tcgcaagtcg
     3361 atcgacgcca tgatcgacga gctcgatcgc gccgagcggc tcggcatcca cgccgtggtc
     3421 ctccacccgg gcgcccacct cggcgccgga gcagatgcgg gagtcgagca gatcgcacgg
     3481 tccctcgatc agattcacgc cgccatcccc aatcacaagg tcgtcacgct gctcgagacc
     3541 gctgctggac agggctcctg cctcggctgc acgtttgacg agctcggaca gattcgcgcg
     3601 cgcgtcgacg acaaggcccg cgtcggcatc tgtttcgata cctgccacgt attcgcctcg
     3661 ggatacgagt tgcgcaaccg cgacgactac gagcggacca tcgactctct cgtcgagcac
     3721 gtcggaatcg acaacgtcgg cgcatttcat ctgaacgact cgaaaaagcc gctcggttcg
     3781 cgcgtcgacc ggcacgagaa catcggtgac ggtgaaatcg gcgtcgatgc cttcgccttc
     3841 ctgctcaacg ataagcgctt tcgcggtatc ccgaaagtca tcgagacgcc gaaaactgtg
     3901 gaacatgagt ccgaccgaaa gaacttgacg ctgctccgat ccctgctgtc gcgatagact
     3961 cttcacccta tggcagaccg catttccaag ccgcaatccg gcacgcgcat cgactacaaa
     4021 gacggcaagc tgctcgtccc cgacgatccg atcgttccat tcatcgaagg agacggaacc
     4081 ggcgcggaca tctggcgtgc cactcgctac gtgctcgacg gcgccgtcga gaagatttac
     4141 ggcggaaaga agaagttcca ctggcacgaa gtgtttgccg gcgacaaggc cagcacacag
     4201 ttcggcgagt acctgccgca ggaaacgctc gacgcgatcg cgcactaccg cgtgtcgatc
     4261 aagggtccgc tctccacgcc ggtcggcggc ggcatccgct ctctcaacgt gacgctgcgc
     4321 caggtgctcg atctttacgc ctgcgtgcgg ccggtgcggt atttcgaagg tgtcccttcg
     4381 cccgtgaaag agcccggcaa ggtggacatg gtgatcttcc gcgagaacac cgaagacgtt
     4441 tacgcgggta tcgagtggcc ggcggagtcc gagggggcga aaagcatcat cgaattcgtc
     4501 aacgggatcg agcggcgcgg cggtgccaag atccgcgaag gatcagccat cggtatcaaa
     4561 ccggtttcag agttcggctc gaagcggctc atccgtaagg caatcgaata cgcgatcgcc
     4621 gcgaaacgcg attcagtcac gatcgtacac aaaggcaaca tcatgaagtt caccgaaggc
     4681 gcgttcaagg attggggtta ccagcttgcg cgcgaagagt tcggcgacgt cacggttcct
     4741 gagtcggaag tgggcccgga aggtgccggc ggaaagatcg tgatcaagga ccggatcgcc
     4801 gattcgatgt tccagcaggt actgctgcgt ccgagcgagt actctgtcat cgccacgacg
     4861 aacctgaacg gcgactatct ttccgatgcc ctcgctgcgc aggtcggcgg cctgggcatg
     4921 gctcccggct cgaacgagtc cgacgaagtg gccatcttcg aagcgacgca cggcacggcg
     4981 ccgaagtacg cgggcaaaga catgatcaat ccgggctcgc tgatcctctc cggcgtgatg
     5041 atgcttcgtc atatgcagtg gtgggacgcg gccgacgcca tcgagcgcgc gatcgcggca
     5101 acgatcgctc agaagaaggt cacctacgat cttgagcgcc agatgaccgg cgcaacgaaa
     5161 gtgtcgacga gtgagttcgg aagagtaatc gtagacaaca tctagctccg ggaggctgtc
     5221 atgaaacgtc tgttgctcac cgcggttgtt gcaacgtttc ctctcatggc ggctgcgcaa
     5281 accgacgctc ccgatctgat catccatcac ggaaaggtct tcaccgctga tccggcgcac
     5341 cgctgggccg aggctgtggc gatccgcgga aaccggatcg tcgcggtcgg aacgaacgcc
     5401 gagatcacgg cgctggggca ggattcgacc aagcgtgtcg acgctggtgg gcgcgtcgtc
     5461 gtccccggct tcaacgatgc gcacacgcat caggggccga agccggaagg tttcggcttg
     5521 tcgctggaaa gcgatcccac atgggcattg acgagcgctg ctctcgtgaa cagcgccgag
     5581 gagacggcag gcgatctgtg gattttcgga acgatcggac ccaaacttct ggccgatcca
     5641 tcggtgaccg cgcaggcact cgacaaggct gccggcggtc ggaaagtcgt gctgtcatcg
     5701 tggacggggc acggcctgat tttcagtacc gcggcgatgg acgcgttgcg gatccgggac
     5761 acggctaccg atcctccggg cggctggttc gagcgcgatg ccaaacagca cgtaaccgga
     5821 aagatgttcg agtacgccgg ctggaacgcc gaacggcgtc tcgcagactc agtcgccgac
     5881 gccgatgcga tcgaacaatt gaagacgttc tccgatcagt cgctggcctt cggtatcacg
     5941 tcggttcaga acatgtcgat gctgcctctg gcgcgctacg aaaagatcga gcgccatggc
     6001 cctgcggcga tccgcatccg gatgatccgc ttcccgatga cagaaaacgg cagtgtgacg
     6061 gaaggaacca acctgtcagc cacggacagg gagcggccac tctcgatcat cagcggaacg
     6121 aagtggatcc tcgacggcac gccagttgag cggggggccg cgattcgccg gccatatccg
     6181 ggcggtggag aggccgtggg gaaactcgac ttcccgcccg acgacatcaa ggccatcctg
     6241 aaagaggcct tcgaatcaaa cgaacagacg ttgctgcatg tcgccggcga tcgcactgcg
     6301 gcggcggtgt tcgatgccat gcgcgctatt ggaccggccg aggagtggcg caagaaaagg
     6361 ctgcgcttcg agcacgggga tggactggag ccggatctcc tcccgctggc gaaggacttc
     6421 ggcatcgtcg tcgtcctcaa tccgtcgcat atcgttgcgc attcgcttta tccggctggt
     6481 ggatacatgc cgttcaagtc gctcttgaag gccggaatcc cgatcgcgat cggctccgac
     6541 ggccctgcaa atccgggcct caatatcatg cttgccacca cgcttccggg caatcctgcc
     6601 gaggcgcttt cccgcgagga agcggttgag gcgtacactc gtggctccgc atacgccgag
     6661 atgatggaaa atgacaaagg cacgctggcc ccggggaagc tcgccgatct ggcgatcctc
     6721 tcgcaggaca tctttcgcgc gtccgcggac aggctcccgg ataccgagag cgtcatgacg
     6781 atcatcgacg ggaaagtcgc tctcgacaac gggacgctca agcgcgaagg cttccccatg
     6841 gcttcgcgaa cgaggtgatc acgatgtttg gtccgtccgt gcgccgcatt ctcatcgcct
     6901 ccgcttttgg gcttgctctc atcgcggcgc ggcagcgcgt cgtgctgcac ccggctccgc
     6961 cgaagatcac gaacggcccg acgttcagta acgaagtcgt gcgcgtcttc caggaccgtt
     7021 gccagagctg ccatcaccct ggcgacatcg ctccgttttc gctgatgacc tacgcggacg
     7081 ccgcgcctca cgccgacgcc atcaaataca tgacacagac gcggcagatg ccgccctgga
     7141 agccgacgcc ggcctgcggc gacttcgccg acgcccgcgt cctcacgcag gacgagatcg
     7201 acctcctcgc gaaatgggct gacaacggcg cgcccgaagg caacgtcgcc gatcttccgc
     7261 ctgcgaaaaa cttcgacggc ggctgggcgc tcggacagcc ggacgtcgtc ctctcgtatc
     7321 ccgagccgta caccccgccg ctcaccagcg acatgtatcg ctgcttcccg atgccgacga
     7381 atctgccggc agacagctac atttcggcca tcgacatcaa gcccggcgac cggcagacgg
     7441 tgcatcacgt gattgcctac atcgatacca ccggtgactc gcagaagctc gacgaggcag
     7501 atcccggccc tggctacacg tcgttcggcg gtcccggctt ctcgctcaac agcctctcct
     7561 caaccctcgg cggttgggct cccggcgcga ggccggcagt tcttccggac gatgtggcca
     7621 tgtcgctgcc ggcaaattcg cgcatcgtgc tgcaggtcca ctatcacccg cacggcgtcg
     7681 cggcggtccc tgaccagaca cagatcggcc tctacttcgc caagaagaag ccgaacaaga
     7741 tcgtctacat gctgccgctg atcaaccaga atttcacgat cccgccgaac aatccgaact
     7801 atctcgtgtc cgccagctac aacgttgcgc tgccggtcca tctctatctc gtcttcccgc
     7861 acatgcacct cctcgggcgc aagatgcacg tcaccgcgac accgtttggt gccgccgagc
     7921 aatgcctcat caacatcgac gactgggact tcaactggca ggggcagtac aaattcagcc
     7981 agcccatcgc cgttacggcc gggacgcggc tatcgctcga ggcgtactac gacaattcga
     8041 ccgacaactg gcgcaacccg aacaacccgc cgaagccagt ctcctggggt gagcagacga
     8101 ccgatgagat gtgcatctcg ttcttcggtt tcacgatcga cagcctcttc gcgactgacc
     8161 agccaccctc ggcgacacag atctttcgcg cggtccaata gtcgtaccga cgcctatacg
     8221 atgcgcaacc tggagagcgc ggccaacttt ccggcttgcg caccatatct ccgggcgtac
     8281 acttcgccat ccccatgaca tctgatcgcg agagcgttct tcgcttcgat ctcgctgtgc
     8341 agaagagcca tccgctcgtc tacaccgcgg cgttttttgc cgcgttggct gcgcaagctc
     8401 tcggcgtttt cgtcctcaac cttcccgtcg caatcgcgat cctgctgatc gagcttgccg
     8461 tcaccgcgtc gctgtatgcg ctgttacgcc gcggcgttga ccggcgcatc ctcaatcccg
     8521 tctggatcgt gactgacatc gttctcgtca cccttggcgt atacgcaagc ggcggcatcg
     8581 ccagcccctg gttcatctgg tacctcggca ccgcagcatc aactgccttc gtactgggta
     8641 agagagcgtc atacctggtc agtctcgcga acgtagttgc gtacgttgcc gtgctgctgc
     8701 tcatggggca ggcggcgttc gtgaactcgg tgctgctgct cgctgtcgtc cggatgctct
     8761 tcctcttcgg ggcctccttt ttctttctcg cagggatcgc caacctgctg cagaagcgcc
     8821 tccagatccg cgagttggaa gccgagcagg cccgcgaagt cgccgagctc acgcggctca
     8881 cgcaggagct ccagcggcga agtgccgagc tggaggatgc cagcctgcgc gtgcaggaga
     8941 ccgatcgtct caagagccag ttcctggcaa acatgagcca cgaactgcgc acgccgatga
     9001 actcgatcat cggtttctcc gagatcctga tcgagcggct cggcgacaag atcgaagaaa
     9061 agcacctcag cttcctgcgc cacattctga cgtccggcca gcaccttctc ggcatcatca
     9121 acgacatcct cgacctctcc aagatcgagg cgggacggat ggagatctac gccgagaagt
     9181 tcgaggtccg ccaagtcatc gaaagcgtct gcgccgtgat ccgcggcatg ggccgcacga
     9241 aagtgccggt gttcaccatc gacatcgatc cgcggcttcc gatcatcgaa actgacctaa
     9301 ccaaattcaa gcaaatcctc ttcaacctgc tgtcaaatgc cgtgaagttt tcgcccggtc
     9361 acgctccgat cgctatctcc gcgtttcact cgggtaacag tcatgacgag ggaacgatca
     9421 ccgtctccgt gcgggatgaa ggagtcggca tcgatccgaa gaaccacgag gtgatcttcg
     9481 atgagttccg gcagatcgac ggaagcgagc gccgcgagtt cggtgggacg ggcctcggcc
     9541 tggcgctcgt caagaaattc gtacagctcc agggcggctg ggtgcgggtc gagagctcgc
     9601 cgggcaaagg aagcacgttc tcattcacac ttcccgtccg ctcgcgcgct gccgtcgtca
     9661 gcgggacgca ggaacttgca tccgctgagt cgcgcaccga accacgtgcg gagcgggtgc
     9721 tggtggtcga ggacgacgcg caggcctacg cgctgatctc gtccgccctc ggctcagccg
     9781 gctatctgtc gattcgcgcc cgccatggcg aagaggcgct caaccttgcc cgcgaaatgc
     9841 gcccggttgc ggtgactctc gatctggtcc ttcccggtct cgacggctgg gacgtgctga
     9901 agacgctgaa aagcgacgcg gcaacctgcg atatcccggt cgtcatcatc tcccgcgtcg
     9961 acgagcgcga cctcggcgtt gcgctcggcg ccaacgatta cttcgtcaaa ccggtcgatc
    10021 gcgaccgtct cctcgaccgc gtccgccaac tcaccgggtc gagtcaatcg aaagcccgtc
    10081 tgctcctgat cgacgacgac gtctccctcc accagctcct cgaagaagag ctgaccaccc
    10141 tcggctacac gatcgaaagc gccttcaccg gcgaaaccgg tttcgccgcc gccaaagcca
    10201 aaacccccga cgtcatcatc ctcgacctca tgatgcccgg catgagcggc ttcgaggtcg
    10261 ccggcctgct gaaagatgat ccctctaccg ccaaaatccc catcctcgtt ctgacctcaa
    10321 aagagatctc cgccgacgac cggcgcgacc tgcagacgaa ggttgccgcg tatgtgcaaa
    10381 agggaggaag cgcgcgcgat caccttgtgg cggagataag aaggttgcaa agggggaatg
    10441 cgtaaatcac ggcgaccgaa acgccttctc gtatccggtc atctcgacgt atccctgacc
    10501 gttgatcgcc acgttgccga acgagccggc gacgtcgacg gcaccttccc agtaggtgac
    10561 ttgcgtcgag cttcgcgtga ccagttcctg atccttcatc aatggctgca gcacgagagc
    10621 gatcccgaac gacggaatgg cgacgcgcca gcccatcgga tacgttgcac ctgatttatt
    10681 cgaatgccag gagccgaggg gcgtgatcac catctggtcg cggcggaggt ggatgacatt
    10741 gccttcgctg ctgatgagcg agccggacga tggtgcgtcg ggcgtgccgt ccttgcggcg
    10801 gatctggtag agcatcagct ccgcgtcgtt gtcgagctgg acggagaacc agtcccaacc
    10861 ctgctggctc tcgcgaagct gcgaactgcc aaactcgtga tccatccacg ccagaccgtg
    10921 gcagctcccg ccgttcacgc ggccacgaac ttctagccgc gacatcgagt agtagtggga
    10981 cgcatatccc accccctcgg ccttgatgct gatgccgttc tcgccgtgaa tcgccggcgc
    11041 tttgcgcgag gtcatgacga gatcgatggc gtccttccct ccgtacgcgg caatccgcca
    11101 cgagccatct gcaagcgtcg tcgcacgcca tccctcgttg aagacatcga gcacaccggc
    11161 cgcggttccg gcagtgaatg gcgacgagcg attgagcttc tcgtagtagc ggaactcgcc
    11221 ggcgccgata tcgctgatgg cgaagtgagc cagcgagaga ttgcgcagat cccatcgcgt
    11281 tgccgtcgga ttgcccggcg gaacgatccc gacgcgaaag aacgtcagct cgaatccata
    11341 gcgctttcca gcctcggtct cgaggtggcc cgtgtagtac caccactccg ttttgtactc
    11401 gtcgtgcgcg ccgtgatcgc gcggaaagga gaagtcgaag ccgggtagcg cggggagaaa
    11461 tgcgttctcc gccgccgtcg caggagtgag aatcacgaag atcggcagca ggagcaggag
    11521 ggctacccag cgcatcacgc ccacggaacg tgcaacattc ggcatccgaa gctcataata
    11581 tgcgctcgcc atggcggaac agccggtcac gatgcctcct cgagcgcgct ttcggacgct
    11641 cgcggcggag acgagcattc ccatcagcgc cgatgagctg atcgcgttgg cgcggcgcta
    11701 cgctgaccag gggatgtacg acgaggccat ccatctgtac gaaatggccg agaagctgaa
    11761 gcccggctcg gtggccgtgc gcatcaactt ggcgcgcacc cgcgatcaaa aagaaaaggc
    11821 cgaggcgtcg cgatatgagg ccgtgcgcca cgaggtcgat tcacagcgcg cccgcgatga
    11881 gatcgatgcc tcgcagtacg ttggtttggc ccagtactac atggccaagg atcagacgtc
    11941 gaaagcgatc gagctgctcg agatcgccaa gctgaagacc ccgaacaact accgtcccta
    12001 cgagatcctg gggcgcctcc actactccca gggcgagtgg gattctgcgc acgaggagat
    12061 cgtcaaagcg cgcaagctca atccgttcga ccgtggactg gctgagatct ccgggcgcgt
    12121 cgagttcgag aagcgcaatt acgagcgtgc gctcgaagac ttcatcgacg cctttcttct
    12181 ctccacggat caaaaaggcg aaagcacaga gccggtccgc cggatgatca acacgctcaa
    12241 acgcatcgag acgatcgaaa cgaaagatct gaacgcccgc atcaagctgc gcgtggaaca
    12301 acttcagaac tgcaccgagc ggcttgagct gcgcaaggac aacctcttca aactggagtc
    12361 gaagaagagc cttcgcgaga tcgtcgacaa gatctcgcgc gacgccgaaa cgcgcgacaa
    12421 cctcatcacg atggccgctg atttgcgcgg tttggccgtc ttccagcaca tcaaagacga
    12481 gcacttgatc cggctctcga aattcgcgcg tgtcgagcat cttgccgaga acgggctggt
    12541 gttcaaggaa gacgaccggt cgatggattt ctatgtcgtc aaggacggac gcatcgagat
    12601 ccgcaaggac acgccgttcg gaccgcagat cctcgggtcg ctcaccgcgg gcgtgatctt
    12661 cggcgagatg aatttcatcg accgcacgca ccgctccagc gacgccgtcg ctgtcattcc
    12721 gtcgagctgc tacaccttct ccttcagcgc gctcgatcag ttgatggacg aggacaaaca
    12781 actcgccgtc ggcctccact gggcgttctg gcgctcgctt acggagaagg tgcgcgatgc
    12841 caacgagcag ctcaagctct tctttcagga ggacgcgaag aagggccagg gccgcaaacg
    12901 cgccatcgaa ggaaagcgcg agatacagca gatcaccgtc aagtcggaag acaaggttga
    12961 tctcttcaaa gagcgcggac tctccgccgg tgaaatgaag ctgctggcga
//
LOCUS       DATLVW010000582         5316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_314863, whole genome shotgun sequence.
ACCESSION   DATLVW010000582 DATLVW010000000
VERSION     DATLVW010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314863"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VNN25_17185"
     CDS             <1..351
                     /locus_tag="VNN25_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93321.1"
                     /translation="FVCYAIEGEVVRLISARRATRFEKRSYMDDESMIRDEPLEPEDE
                     ETMHFDWSKAVRGLHYIPREKGTATLDEDVYAIFRTSEEVNNALRMLIRENRIPHFLS
                     DEEWYAKHKKSRQD"
     gene            complement(337..1287)
                     /gene="arcC"
                     /locus_tag="VNN25_17190"
     CDS             complement(337..1287)
                     /gene="arcC"
                     /locus_tag="VNN25_17190"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXH93322.1"
                     /translation="MPSPTKLAVVAFGGNALLRPEDRGTQEEQIARAKQAARWLAEIV
                     RHGYKLIVVHGNGPQVGNILVQNEEASTKIPPQTLDVCVAQTEGSMGFLLQQAIRNRL
                     DSIGVSGDVATILTEVEVDPADAAFKRPTKPIGPSVTRYRAEALERDLGWTMREDAGR
                     GWRHVVPSPRPLRILNLQTIVHMLASAAVVIAAGGGGIPVVRGRDGQWRGIEAVIDKD
                     FASSLLASELHAEMYVVLTGVAKVSIDYEKPSEKALDRMTVAEATRYLAEGQFPAGSM
                     GPKIEAAIQFVTRGGKQVLITDVEHLRDALQGNDGTLVLA"
     gene            complement(1289..2554)
                     /locus_tag="VNN25_17195"
     CDS             complement(1289..2554)
                     /locus_tag="VNN25_17195"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HXH93323.1"
                     /translation="MQLNVTSEIGRLKSVLVHLPGREIDVMIPPMMNQLLFDDILYGQ
                     VAREEHRRFQQLIRFIAPEVIDIQDLLEEVLESDEGKRLVIRDLENRKIFGRKLASAL
                     REQPAALLAQTLIGGIPHERDGKGELPSFDLLPVPNYFFMRDPQVVIGNGVVVCGMAT
                     QARSRESLLSGYVFAHHPLFRESRRWSDFTAGIAPGREHKRMPTIEGGDILVPSRDLL
                     LVGVTERTNRAGVRQLATSLREAGSEVKTIIVVELPRQRSFMHLDTVFTFTSRNECLI
                     YPPVILPGGSQAATVTTIDLTKRGISYSPQKSLLAALKKRGFDLDPIYCGGRSAVDQQ
                     REQWTDGANAFTLAPGIILLYERNVRTAEELAAHGYHIVYEDDLLLGRTELETWTDTK
                     YAIQMAGHELSRARGGPRCMTMPLVREDV"
     gene            2593..3504
                     /locus_tag="VNN25_17200"
     CDS             2593..3504
                     /locus_tag="VNN25_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93324.1"
                     /translation="MSKIAGPMKVQWFLPAALLLYSGIAHASPPRISFTRTLPAMRDL
                     GPGERLSVIYAIGDSNKIDTFLDRFVDLVSRAGAVRIENAVENNHHLFFDKRSLPVLR
                     RNHPADIYVGVNRFSCSGIEKSAEGSEHLENGERIKRLHHWIDSTCSARIDVLSVDGK
                     RLFSYSVHGDGTSPRVVTLTDEERDIAYEQAARFAAVIAADEITPRTLRETIELDESA
                     PSFDVAFAMIGSERLTDARSIWQAAAARHQDSAALFFDLAAVSEAIGDVEGARGYYER
                     AVRLSPKERRYATEFRLFRRRNAGIRK"
     gene            complement(3554..4021)
                     /locus_tag="VNN25_17205"
     CDS             complement(3554..4021)
                     /locus_tag="VNN25_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXH93325.1"
                     /translation="MTYLTRSSNPRNDFRVLQARVFEPFFGRFNFLEDELKSGTWAPP
                     VDVAEEKDTIIVRVEVPGMNEQDLKVSFEDGVLTVSGERQFERKEDRNYHRIERTYGT
                     FSRTFSLPRTVDQDSIAASYRDGILEITIPKREEAKPKQIQINATAGAVPVNQ"
     gene            4150..>5316
                     /locus_tag="VNN25_17210"
     CDS             4150..>5316
                     /locus_tag="VNN25_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HXH93326.1"
                     /translation="MTALPPVSAPPELSREVARRRTFAIISHPDAGKTTLTEKLLLYG
                     GAIEMAGAVRARKGQRHATSDWMAMEQTRGISITSTALQFEYEGCYLNLLDTPGHEDF
                     SEDTYRTLAATDSAVMVLDSAKGIEPQTRKLFEVCRMRQIPIICFINKLDHPGRDPFD
                     LLSEIEKELHVGAVPMNWPIGNGPNFQGVFDLRKQQVLRFERAAHGASVAAVKVGGLD
                     DPMLEDEIGPTAWHELREAAELLSGAGIAFTPQEFGHGRVIPVYFGSAINNFGVQPFL
                     DALLEFAPPPAPHPAHVGGEETIVEATDEMFTGFVFKIQANMDKRHRDRLAFMRVCSG
                     VFEKDMLVHHSRLGREVRMTRPHRLFAREREGIETAWPGDVIGFVNPGLFRIGDA"
BASE COUNT          990 a         1643 c         1660 g         1023 t
ORIGIN      
        1 ttcgtttgtt acgcaatcga aggcgaggtt gtacgcctga tcagcgcgcg tcgcgcaaca
       61 cgttttgaaa agaggagcta tatggatgat gaatcgatga ttcgcgacga accgctcgag
      121 cccgaagatg aggagacgat gcacttcgac tggagcaaag ctgtgcgcgg tcttcactac
      181 atcccgcgcg aaaaaggcac agcgacgctc gatgaggatg tttacgccat cttccgcacg
      241 tcggaggaag taaacaacgc gcttcggatg ctcatcaggg agaaccggat cccgcacttt
      301 ctctccgacg aggagtggta cgcgaagcac aagaagtcac gccaggacta gtgtgccgtc
      361 gttgccctgc agcgcgtcac ggaggtgctc aacgtcggtg atcagcacct gtttgccgcc
      421 gcgggtgacg aactggattg cggcttcgat cttcggtccc atcgagccgg cgggaaactg
      481 gccctcggcg agataacgcg ttgcctcggc gaccgtcatg cgatcgagcg ccttctcgga
      541 cggcttctcg tagtcgatcg acaccttggc cacgccggtc aggacgacgt acatctcggc
      601 gtgcagttct gaggcgagaa ggctcgatgc gaaatccttg tcgatcaccg cctcgatgcc
      661 tcgccactga ccgtcgcgtc cgcgcacgac cgggattccg ccgcctccag ctgcgatcac
      721 gacggcggcg ctggcgagca tgtgcacgat cgtctggaga ttgaggattc ggagcggccg
      781 cggagaaggg acgacatgcc gccatccgcg cccggcgtct tcgcgcatcg tccacccgag
      841 gtcgcgctcg agtgcctcgg cgcgataacg cgtgacggac ggtccgatcg gcttcgtcgg
      901 acgtttgaag gccgcatcgg cgggatcgac ttccacctcg gtcaggatcg tcgctacgtc
      961 gccgctgacg ccgatgctgt cgagccggtt gcgaatggcc tgctgcagca ggaatcccat
     1021 cgacccctcg gtctgcgcca cgcagacgtc gagcgtctgc ggcgggatct tcgtcgacgc
     1081 ctcttcgttc tgcacgagga tgttgccgac ctgggggccg ttcccgtgga cgacgatcag
     1141 cttgtagccg tgacgaacga tctcggcaag ccaacgtgcg gcctgctttg cgcgcgcgat
     1201 ctgctcttcc tgcgttccgc ggtcctcggg gcgcagaagc gcgttgccac cgaacgccac
     1261 gacagcaagt tttgttggcg acggcacgct acacgtcctc gcgaacgagc ggcatcgtca
     1321 tgcagcgggg gccgccgcgt gcgcgcgaca gctcgtggcc ggccatctgg attgcgtact
     1381 tggtgtcggt ccacgtttcc aactcggtac gcccgaggag gagatcgtct tcataaacga
     1441 tgtgatagcc gtgcgccgcg agctcctcgg cggtacggac gttgcgctcg tacaacagga
     1501 tgatgccggg agcgagcgtg aacgcgttcg caccgtcggt ccactgttcg cgctgctgat
     1561 ccaccgcgct ccgaccgccg cagtagatcg gatcgagatc gaaaccacgt ttcttaagcg
     1621 cggcaaggag cgacttttgc ggtgagtacg agatgcccct cttcgtcagg tcgatcgtgg
     1681 tgacagtggc tgcctggctg ccgcccggca ggatgacagg cgggtagatc aggcactcat
     1741 tgcggctcgt gaacgtgaag acggtgtcga gatgcatgaa cgaccgttgc cgcggcagct
     1801 cgacgacgat gatcgtttta acctcgctgc cagcctcgcg cagagacgtg gccaactgcc
     1861 gcacacccgc cctgttcgtc cgttcggtca ctccgaccag gagcagatcg cgcgagggca
     1921 cgagaatgtc gcccccctcg atcgtcggca tgcgtttgtg ctcgcggccc ggggcaatgc
     1981 cggccgtgaa gtccgaccaa cggcggctct cgcgaaacag cgggtgatgc gcgaacacgt
     2041 agccagacag gagcgactca cggctccggg cctgcgtggc catcccgcaa accaccacgc
     2101 cgttgccgat caccacctga ggatcgcgca tgaaaaagta attgggaaca ggcaggagat
     2161 cgaacgaggg gagctcgccc ttgccgtcgc gctcatgagg gatgccgccg atcagcgtct
     2221 gcgcaagcag tgccgccggc tgttcgcgaa gcgcagacgc cagtttgcgg ccaaagatct
     2281 tgcgattctc gagatcgcgg atgactaggc gcttaccctc gtcgctttcc agcacttctt
     2341 cgagcagatc ctggatgtcg atgacctcgg gagcgatgaa gcggatgagc tgctggaacc
     2401 ggcggtgctc ctcgcgggcg acctgtccgt aaaggatgtc gtcgaacagg agctggttca
     2461 tcatcggcgg gatcatcacg tcgatctccc gcccgggcag gtggaccagc acgctcttca
     2521 gcctgccgat ctcactcgtg acattcaact gcattcgggc gcgattctat cgcagcgatt
     2581 tgcgcgcgga gtttgagtaa gattgcagga ccgatgaagg tgcaatggtt cctgccggct
     2641 gcgctcttgc tgtattccgg catcgcgcac gcctcaccgc cgcgaatctc gtttacgcgt
     2701 acgctgccgg cgatgcgcga cctcgggccc ggcgagcgcc tctcggtcat ctacgcgatc
     2761 ggcgacagca acaagatcga taccttcctc gaccgcttcg tcgatctggt ttcgcgcgca
     2821 ggcgccgtgc ggatcgagaa tgcagtcgag aacaatcacc acctcttctt cgacaaacgc
     2881 tcgcttccgg tccttcgcag aaaccatccc gccgatatct acgtgggggt gaaccgcttc
     2941 agttgttcgg gtatcgaaaa gagtgccgaa ggcagcgagc acctcgaaaa cggcgagcgg
     3001 atcaagcgcc tgcatcactg gatcgactct acttgctcgg cccgcatcga cgtgctgagc
     3061 gtcgatggaa agagactgtt ttcgtattcc gtgcacggcg acggcacttc gccgcgggtg
     3121 gtgactttga ccgatgaaga gcgggacatc gcctacgaac aggctgcccg ctttgccgcg
     3181 gtcatcgccg cggacgagat cacgccgcgc acgttgcgcg aaacgatcga gctggacgag
     3241 agcgcgccgt catttgacgt ggcctttgcg atgatcggct cggagcgtct gaccgatgcg
     3301 cgctccatct ggcaggccgc ggcagcacgg catcaggact cggcggcgct cttcttcgat
     3361 cttgctgcgg tctcggaggc tattggggat gtcgaagggg cgcgcggcta ctacgagaga
     3421 gcggtgcgcc tttcaccgaa agagcggcgc tacgccaccg aattccggct ctttcgccgc
     3481 cgcaacgctg gcatacgcaa atgaaaaggc cgccgtttcc ggcggccttt catgacgcga
     3541 tcgcaagaat ctgttactgg ttgacaggta ccgcgccggc cgttgcattg atctgaatct
     3601 gcttcggctt cgcttcctca cgcttcggaa tcgtgatctc gaggatgccg tcacggtagc
     3661 tggcagcgat cgagtcctga tcgaccgtac ggggcagcga gaacgtgcgg ctgaacgtgc
     3721 cgtaagtccg ttcgatgcgg tggtagttgc ggtcttcctt gcgctcgaac tgccgctcgc
     3781 cgctgacggt taggacgccg tcttcgaagc tgaccttgag gtcctgttcg ttcatcccag
     3841 ggacttcgac acgcacaatg atggtgtctt tctcctcggc gacatccacc gggggtgccc
     3901 aggtcccgga cttcagctca tcttcgagga agttgaagcg gccgaagaag ggttcaaaga
     3961 cacgggcttg gaggacacgg aaatcgttgc ggggattgct ggatcgggtg agataagtca
     4021 tggattgcct ccctttgata tatgagcttt atgcgctcat atcgcactaa accttagctg
     4081 tttgaactca tattcccaag atggacagct cggaatgttc aagtgaccac cctgttctaa
     4141 cggactcgta tgacggcact tccgccagtt tccgcgcctc cggagctcag ccgggaggtc
     4201 gcccgccgcc gcacgttcgc gatcatttcg caccccgacg ccggcaaaac gacgcttacg
     4261 gagaagctgc ttctttacgg cggcgccatc gaaatggccg gcgctgtccg cgcgcgcaaa
     4321 gggcagcgcc atgccacctc ggactggatg gcgatggagc agacccgcgg aatctccatc
     4381 acctcgacgg cactccagtt tgaatacgaa ggttgctacc tcaacctcct cgacacgccc
     4441 ggtcacgaag acttcagcga agacacctac cgcacgctgg ccgccactga cagcgctgtg
     4501 atggtgctcg actcggcgaa aggcatcgag ccgcagacac ggaagctctt cgaggtttgc
     4561 cgcatgcggc agatcccgat catctgtttc atcaacaagc tcgatcatcc cggccgcgat
     4621 ccattcgacc ttctgagtga gatcgaaaag gagctgcacg tcggcgcggt accgatgaac
     4681 tggccgatcg gcaatggtcc gaactttcaa ggcgtgttcg acctgcgcaa gcaacaggtt
     4741 cttcgattcg agcgcgcggc gcacggcgcg tccgtggccg cggtgaaagt gggcggtctt
     4801 gatgatccga tgttggaaga cgagatcggc ccgacggcgt ggcacgagct gcgcgaagcg
     4861 gccgagcttc tctcgggcgc agggatcgcg ttcactccgc aggagttcgg gcacggacgc
     4921 gtgatccccg tctacttcgg aagcgccatc aacaacttcg gcgtacagcc gtttctcgat
     4981 gcgctgctcg aattcgcgcc gccacccgct ccgcatccgg cacacgtggg tggcgaagag
     5041 accatcgtcg aggcaacgga cgagatgttc accggcttcg tcttcaagat ccaggcgaat
     5101 atggacaagc gccaccgcga ccgcctggcg ttcatgcgcg tctgttccgg cgtcttcgag
     5161 aaggacatgc tggtgcacca ctcccgactc ggacgcgaag tacggatgac gcggccgcat
     5221 cgcctcttcg cacgggagcg ggaggggatc gagaccgcat ggccgggcga cgtaatcggc
     5281 ttcgtgaacc cgggcctgtt tcgcatcggt gatgcg
//
LOCUS       DATLVW010000583         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_395337, whole genome shotgun sequence.
ACCESSION   DATLVW010000583 DATLVW010000000
VERSION     DATLVW010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395337"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VNN25_17215"
     CDS             complement(<1..906)
                     /locus_tag="VNN25_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXH93327.1"
                     /translation="MEQPTYVYYFGDGHAAGNAQMKDVLGGKGAGLAEMTNIGVPVPP
                     GFTISTAVCTYFYAHGKTYPSELKDAVAENLGKVEQSVGRKFGDREKPLLVSVRSGAR
                     ASMPGMMDTILNLGLNDDTVQGLATSSKDERFALDSYRRFIQMYSGVVLEIDKDHFEH
                     ELFSLRDKSGVKTDAEIPADGLRGLVDTYKGIVREKSGREFPQDVHEQLWGAIGAVFN
                     SWNNQRAITYRKLNQIPDAWGTAVNVQSMVFGNMGDDCATGVAFTRNPANGEHRFFGE
                     YLPNAQGEDVVAGIRTPLPISKAQAI"
     gene            complement(917..2134)
                     /locus_tag="VNN25_17220"
     CDS             complement(917..2134)
                     /locus_tag="VNN25_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXH93328.1"
                     /translation="MKSRLLLALLLFASCSRPAPERAAKAKEPGELPTDIINEQSSLL
                     DLSRGATIVSRTGETMLPVSAVSTIDGDVGTFWQTPPHDFPQSIVIALGAPSRIDRIG
                     IRSLEVGFEPKSVPFESSLDGTTWQPLATITPKRSELAQWLDVTPTTALYLRVNIPAP
                     TTEGHDVRLHSIYARGAEIAPRVDPPIDGCWSINGSASLFVQRGIHATGVLMRGAQPI
                     FLEGGSTGRVWRFNWIHGNDFGCAALAVSPDGKHFSGIEWHEEAIPLFRGMPWFGERT
                     TCTPFVMRNDVELALLHRSNRVALFGLQFDASGNLLRDPSREELQSLLKIIRAAPPID
                     IIAHEFRQPDARRNKEVAQHEIDSVKAALVSLGADMSRISFRAAGSDDPRQKPESDIA
                     RVVYSSVEVEVRR"
     gene            complement(2131..>3297)
                     /gene="glyS"
                     /locus_tag="VNN25_17225"
     CDS             complement(2131..>3297)
                     /gene="glyS"
                     /locus_tag="VNN25_17225"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXH93329.1"
                     /translation="PDGNAAYGNSFVTNARFADAKFFYETDRKRTLESRRDQLSHLQF
                     QEKLGNYLQKTDRIEQIATAITDDPETLAAARLCKADLVTEMVKEFTELQGNIGGIYA
                     REEGMPENTWQAVYDHYLPINIDDALPRTRSGAVVSLADKIDTLAGFFLVGARPTGSK
                     DPFALRRAAQGVVQILLNRDKRSVNIGLDKLIDVALQSHEQPSNPATQQLKADLLTFF
                     AERIRTLLEASAYGFAYDEIAAAMEAGWESSLTDLVDRIAAVKAMRNEPNFLSILDSA
                     KRIANITAGQQIAANIDPSKLENDVERRLASLAAIVGTQIEEMIAERDYKRALETFAA
                     MAPELETFFAEVMVMVEDEALRRNRMALLHKIGSAVAGIADVTKIVVDRREYRT"
BASE COUNT          602 a          950 c         1062 g          683 t
ORIGIN      
        1 aatcgcctga gcctttgaga tcggcagcgg cgtgcggatc ccggctacaa catcttcgcc
       61 ctgcgcgttc ggaaggtatt cgccgaagaa gcgatgctca ccgttggccg gattgcgggt
      121 gaaggcaacg ccggtggcac agtcgtctcc catgttgccg aagaccatcg actgcacgtt
      181 caccgctgtg ccccaggcat cagggatctg gttgagcttg cgataagtga tggcgcgctg
      241 gttgttccat gagttgaaaa ccgcgccgat ggcgccccag agctgctcgt gtacgtcctg
      301 cggaaactcc cgtccgctct tctcgcgcac gatgcctttg tacgtgtcga cgagaccacg
      361 aaggccatca gcaggaatct cggcgtcggt cttgactccc gacttgtcgc gcagcgagaa
      421 gagctcgtgc tcgaagtgat ccttgtcgat ctcgagaacg acgccggagt acatctggat
      481 gaagcggcgg taggagtcga gtgcgaagcg ttcgtctttc gacgaggttg ccagtccctg
      541 caccgtgtcg tcgttgaggc cgagattaag gatcgtgtcc atcatgcccg gcatcgaggc
      601 gcgagccccg gagcggacgg agacgagaag cggcttttcg cggtcaccga acttgcggcc
      661 gaccgactgt tcgaccttgc cgaggttctc ggcgacagcg tccttgagct cggatggata
      721 ggtcttgccg tgcgcgtaaa aataggtgca gacggccgtc gagatggtga agccaggagg
      781 caccggaacg ccaatgttgg tcatctcggc gaggccagcg cctttgccgc cgagtacgtc
      841 cttcatttga gcgttgcccg cggcgtggcc gtcaccgaaa tagtagacgt aagttggctg
      901 ctccattgcg gcgcgattat ctccgaactt ctacctcgac gctgctgtag accacgcggg
      961 cgatatcgct ctcgggcttt tggcgcggat cgtcgcttcc cgccgcgcgg aaggaaatgc
     1021 gtgacatgtc agcgccgagg ctcaccaggg cggccttgac ggaatcgatt tcgtgttgcg
     1081 caacctcttt gttgcgacgc gcatcgggtt gacggaactc atgcgcaatg atgtcgatcg
     1141 gcggcgccgc gcgaatgatc ttcagcagcg actgcaattc ctcacggctg ggatcgcgaa
     1201 ggaggttgcc cgatgcatcg aactgcagcc cgaacagcgc gacccggttg gagcggtgaa
     1261 gcagcgccag ttcgacgtcg ttccgcataa cgaagggagt gcaggttgtc cgttcgccga
     1321 accacggcat tccgcgaaac agggggatgg cctcttcatg ccattcgata ccgctgaagt
     1381 gtttgccgtc cggcgagact gcaagtgcgg cgcaaccaaa gtcgttgccg tgaatccaat
     1441 tgaatcgcca gacgcgtccc gtcgagcctc cttcgaggaa gatcggctgt gctcctcgca
     1501 tcagcacacc tgtcgcgtga atacctcgtt gaacgaacag ggaggcactg ccgttgatcg
     1561 accagcagcc gtcgataggc ggatcgacgc gaggcgcgat ctccgctccg cgagcgtaga
     1621 tcgagtggag tcggacgtcg tgcccctcag tcgtgggcgc cggaatgttg acgcgcagat
     1681 acaaagctgt cgttggcgtt acgtccagcc actgcgcaag ctcgctccgc ttcggtgtga
     1741 tcgtagcgag gggctgccag gtcgtgccgt cgagcgagct ttcgaacggc acgctctttg
     1801 gttcgaagcc tacttccagc gaacggatcc cgattcgatc gatacggctc ggtgcgccga
     1861 gagcgatgac gatcgactgc gggaaatcgt gaggaggagt ctgccagaac gtgccgacgt
     1921 cgccgtcaat cgtgctgacg gccgacacgg gcaacatcgt ctccccggtc cttgaaacga
     1981 tcgtcgctcc tcgggagaga tcgagaagac tcgactgctc attgatgatg tcggtgggca
     2041 gctcgcccgg ctctttcgcc ttcgcagcgc gttcaggggc agggcgcgaa cagctcgcga
     2101 agagaagaag cgcgaggagc aggcgcgact tcacgtccga tactcgcgcc ggtcgacgac
     2161 gattttggtg acgtcggcga tgccggctac ggcactgccg atcttgtgca agagcgccat
     2221 ccggtttcgc cgcagtgctt cgtcctccac catgaccatg acttcggcga agaatgtctc
     2281 cagctccggc gccatggccg cgaatgtctc gagagcacgc ttgtaatcgc gctcggcaat
     2341 catctcctcg atttgtgtgc cgacaatcgc ggcaaggctg gccagacggc gctcaacatc
     2401 attctccagc ttcgacggat cgatgtttgc cgcaatctgc tggccggccg tgatgttggc
     2461 aatgcgcttt gcggaatcga ggatggagag gaagttcggt tcgttgcgca tcgctttgac
     2521 cgcggcgatg cgatcgacga gatcagtgag tgacgactcc catcccgctt ccatcgctgc
     2581 ggcgatctcg tcgtatgcga atccataggc ggacgcttcg agtagcgtgc gaatacgctc
     2641 cgcgaaaaag gtgaggaggt cggctttgag ttgctgcgtt gccgggttgc tgggttgctc
     2701 gtgcgattga agcgcaacgt cgatcagctt gtcgagaccg atgttcacgc ttcgcttgtc
     2761 gcggttgagc agaatctgca cgacgccctg tgcggcgcgg cgaagagcga acggatcttt
     2821 tgagcccgtc ggtctcgccc caacgaggaa gaatccggcc agtgtgtcga tcttgtcggc
     2881 gagtgagacc actgcgccgc tgcgcgtgcg aggtagcgcg tcatcgatgt tgatgggaag
     2941 gtaatgatcg tagacggcct gccaggtgtt ctccggcatg ccttcttccc gagcgtagat
     3001 gccgccgatg ttcccctgca gttcggtgaa ctccttcacc atttcggtga cgagatcagc
     3061 cttgcaaagg cgtgcggcag cgagtgtttc gggatcgtcg gtgatggcgg tggcgatctg
     3121 ctcgatgcga tcggtcttct gcaggtagtt cccgagcttc tcctggaact gcagatggga
     3181 gagctgatcg cggcgcgatt cgagcgtccg cttgcgatcg gtctcgtaga aaaacttcgc
     3241 atcggcgaat cgggcgttgg tgacgaacga gttgccgtac gcggcgttgc cgtcagg
//
LOCUS       DATLVW010000584         6100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_560861, whole genome shotgun sequence.
ACCESSION   DATLVW010000584 DATLVW010000000
VERSION     DATLVW010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560861"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VNN25_17230"
     CDS             <1..757
                     /locus_tag="VNN25_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH93330.1"
                     /translation="PRSNPATYTNVFNCIRDLMSQVPDARMRGYGPGRFSFNVKGGRC
                     EACRGDGQIKIEMHFLPDIYVTCDVCGGKRYNRETLQVTYKGSSIAEILDMTVEQGLE
                     TFKNIPKIANILRTVDEVGLSYIKLGQSSTTLSGGEAQRVKLAKELAKRATGRTLYIL
                     DEPTTGLHFDDVSKLLHLMHGLVDRGNTVVVIEHNLDVIKTADHVIDLGPEGGDGGGY
                     IIATGTPEQIAKTKGTPTGEYLKKMLAKSGVRA"
     gene            1019..1744
                     /locus_tag="VNN25_17235"
     CDS             1019..1744
                     /locus_tag="VNN25_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93331.1"
                     /translation="MDTFASHDINSPGSITRQVIQCLLESDLVVANLTGLNPNVMYEL
                     AVRHSTRRPVVTIAEAGTVLPFDLADERTIFYINDLGGARELARRLLVACRAALAEPT
                     PDNPIYRATDRSITSTMGPGTTEKIFLERFGALEKLVSERFASLEERLASERQPLSIT
                     SHVTRDVYNLSVRGALQQVRALLAELDDWWEVIGEPHYQGGNDGIYIVYFTALHHLVP
                     SRVSQLANGSGLQVIGIKVRSVD"
     gene            complement(2044..2364)
                     /locus_tag="VNN25_17240"
     CDS             complement(2044..2364)
                     /locus_tag="VNN25_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH93332.1"
                     /translation="MKTEKVKRLEKAGWKVGDAKDFLGLDASEAEFVEIKLGLARRLR
                     ELRAQHSWTQADLAARLGSSQSRVAKMEAADSTVSVDLLVRSLLTAVADRKDVGRAVG
                     SRRS"
     gene            complement(2367..2732)
                     /locus_tag="VNN25_17245"
     CDS             complement(2367..2732)
                     /locus_tag="VNN25_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH93333.1"
                     /translation="MPDLTYAVVRDGGQTARLAAGEVKTPPFSRVGRVEAGFLLRRLQ
                     QGEQLSLPSSRPMPMIGARCHELRIVDREATWRILYHVADDAVVILEVFSKKTRATPQ
                     RVLDAARSRLEKYLRDVEG"
     gene            3267..4211
                     /locus_tag="VNN25_17250"
     CDS             3267..4211
                     /locus_tag="VNN25_17250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXH93334.1"
                     /translation="MAGNQMSADSKRTIAVFFTALVIVSGFFDFMAVHYKGGSYNVMW
                     SVTIATLLAFWITRRDLRTLGWGWGPWRYQWMAFLIPLAYCAIGYALIWAKGWGGFYN
                     PDFAVQLRGKYGLTGWSDSAVLGYVLPLTAFLGMPGSLSSALGEEIGWRGFLVPELAK
                     SMTFSKVVLISGVIWGAYHLPLILLGTYHNNSSNALPFAGQLVMFFTVIMSASVIIAY
                     LRLKSGSLWSAAVFHASHNLFVQTIFNPITIQYPNTPKYVDELGLVLPLTMLPVAVYF
                     FVKGRREFGVIAVGQSMQAVCPAIIDDGFESGSPSSIR"
     gene            complement(4401..4937)
                     /locus_tag="VNN25_17255"
     CDS             complement(4401..4937)
                     /locus_tag="VNN25_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93335.1"
                     /translation="MQPSRPLTINGQPAVCVQYVTAAAGDASVVVYAIEAVDRPKYSD
                     FPHQMFVALGKRRADNAWKLSDRHDVTATLLSAEDFGNFIVMDARVDRFTLAGARFVD
                     VGVSATISGSGGISATKDLLFRVEGGKLLPAATLETEGYARGGVSFLHQITSELLVGK
                     DEVVRSSATVSPEAGISR"
BASE COUNT         1256 a         1866 c         1745 g         1233 t
ORIGIN      
        1 gccgcgctcg aacccggcga cgtacacgaa cgtcttcaac tgcatccgcg acctgatgtc
       61 gcaggtgccc gacgcgcgca tgcgcggcta cgggccgggg cgcttctcct tcaacgtgaa
      121 aggcggccgg tgtgaggcgt gccgagggga cgggcagatc aagatcgaaa tgcacttcct
      181 ccccgacatc tacgtcacat gcgacgtctg cggcggcaag cgctacaacc gcgaaacgct
      241 gcaggtcacg tacaaaggga gctcgatcgc cgagatcctc gacatgacgg tcgagcaggg
      301 cctggagacg ttcaagaaca tcccgaaaat cgccaacatc ctccgcaccg tcgacgaggt
      361 aggcctcagc tacatcaagc tcggccagtc gtccacgaca ctctccggcg gcgaagcaca
      421 acgcgtcaag ctggccaaag agctggccaa acgtgccacc ggccgaacgc tctacatcct
      481 cgacgagccc acgaccggcc tgcacttcga cgacgtctcc aaactcctcc acctgatgca
      541 cggcctggtc gaccgcggca acacagtggt cgtcatcgag cacaacctcg acgtcatcaa
      601 aacagcggat cacgtcatcg acctcggccc cgaaggcggc gacggcggcg gctacatcat
      661 cgcgacgggc acgccggagc agatcgcgaa aacgaagggc acgccaacgg gggagtattt
      721 gaagaagatg ctcgcgaaga gtggggtgag ggcgtaaggc acgagtcagt tcggtataca
      781 cgatgtagcg ctacccggct ggtgatgcac ggcaacctaa cgccgcaaac ttataccgtg
      841 tcctctccct aatccccatc ctgacccact acccgcgtac acttgcctcg tggagtcgcc
      901 aaagaatcat cgtcaatgct tcgtcattac tccgatcgga gccgagggca cagctactcg
      961 ccgatctacc gatggactaa tctcgtcggt cattaagcca accctcgatg aattccaaat
     1021 ggacaccttt gcctctcatg atatcaactc gcccggttcg attacacgcc aagtcatcca
     1081 atgccttctt gagtcggact tagtcgtagc aaatcttact ggcttgaatc caaatgtaat
     1141 gtacgagctg gccgtccgcc attcaacaag acgaccagtg gtcacgattg cggaagcagg
     1201 aactgtcctt ccatttgatc tagcggacga acgcacaatc ttctacatta acgatctcgg
     1261 cggggctcgg gagctcgcac gacgattgct cgtggcctgt cgcgctgctc tcgccgagcc
     1321 gacgcctgat aatccgatct atcgcgctac ggatagatca attacgtcga ccatgggccc
     1381 gggcactacc gaaaagatat tcttggagcg ttttggcgcg ttagagaagc tcgtgtctga
     1441 gcggtttgca tcgcttgagg agcgtctagc atcagagcgc cagcccttga gcatcacgtc
     1501 gcacgttaca agggatgtct ataacttgag tgtcagaggc gcccttcaac aggtacgcgc
     1561 tcttctcgcg gagctcgacg actggtggga agtcatcggc gagccgcact accagggcgg
     1621 caacgatggc atttacattg tctatttcac ggctttgcat catctggttc cctcacgtgt
     1681 cagtcaattg gcaaacggat ctggacttca agtgataggc ataaaagtac gatcagttga
     1741 ctgacaattt cagacgacga cgcctcctcc gcgtccagcc tcacctctga ccgcgtgact
     1801 gtggcggtga atggggtcag gtctgaagta tgaagtgacg gatgacggag cctggcctct
     1861 tgcgaccaat taccaccgtc acttcatact tcagacctga ccccacctcc gttcgccgag
     1921 tagcaacaca tcgcaagcgt ggacgcactt agtcggacgc gaaactggca ccacgtcggg
     1981 ctgtcaacgc gaccttgatg gcagattctc catttcggaa ccggctgtat accggctgct
     2041 ccgttaactc cgccgtgatc caaccgcacg cccaacgtct tttcgatccg ctacggcggt
     2101 cagcagcgag cggacgagca agtccacgct gaccgtggaa tcggccgctt ccatctttgc
     2161 cactcgggat tggctggagc cgaggcgggc ggcgagatcg gcctgggtcc agctgtgttg
     2221 tgcgcgtagt tcgcggagcc gccgggccag tccgagtttg atctccacaa actctgcttc
     2281 cgaagcatcg agccccagga agtcttttgc atcaccaact ttccagcccg ctttctccag
     2341 cctcttcacc ttttccgttt tcatggtcag ccctccacgt ctcgcaagta cttttccaat
     2401 cgcgatcttg ccgcgtccaa cacgcgttgc ggcgtggctc gcgtcttctt cgaaaacact
     2461 tcgagaatca cgactgcgtc atcggccacg tgatacagaa tccgccatgt agcctcacgg
     2521 tcaacaattc gcagctcgtg gcatcgcgca ccgatcatcg gcatcggacg cgagctcggc
     2581 agcgacagtt gctctccttg ctgcagacgt cgcagaagga aacctgcctc cacccgtccc
     2641 acacgagaga atggcggggt cttcacctcg ccagcagcca gacgagcggt ttgtccaccg
     2701 tcacgaacga cagcgtatgt cagatccggc atgccagcaa gtgggcttag gaccgttcct
     2761 gacgtgatcc gcacccccgt ttcccgaagg cgacgaacaa ccgatccaat cgagctcggt
     2821 gtcatcctga gactgccgga gccgcccgct cgaattgcgt tgtttgcgca gcgcgtgctc
     2881 ggcggcggag gacggcgaag gatctcaaac ttcggggatc gtgttcgccc agcgcgagcg
     2941 aacgtgatca aaggcactcg aacgagatct tcgcattttg agatccttcg ccgtcctgcg
     3001 gcgccgaggg aaagtcagcg cgagggttat gagtagcgcg ggcgcctccg gctggctcag
     3061 gatgacacct cacgtctgag cgtgtcgtca ccgagacgcc ggcgggggag tgtttgaaga
     3121 agatgttggc gaagatcaag tttcctgttt gttagcgaca gtatgccggc tttggaacgg
     3181 gcgattgcga aacaccggtt cctggtatcc gtaagactgc catcgccgcc gcatcaggag
     3241 tgaacggtga cgatcaggag agcctgatgg ccggtaacca aatgagcgcc gactcgaaac
     3301 gcacgattgc cgtcttcttc acggccctgg tgattgtctc cggcttcttc gatttcatgg
     3361 ccgtgcacta caaaggcggc tcatacaacg tgatgtggag cgtgaccatc gccacgttgc
     3421 tggccttctg gatcaccagg cgcgacctgc gaacgctggg ctggggctgg gggccctggc
     3481 gctatcagtg gatggcgttc ctgatccctc ttgcctattg cgccattggc tacgcgttga
     3541 tctgggcgaa ggggtggggc ggcttctaca acccggactt cgccgtccag ctgcgcggca
     3601 agtatggcct gacaggatgg agtgacagcg cggtcctggg ctacgtcctg ccgttgacgg
     3661 ccttcctggg catgccggga agtctatcca gcgcgctcgg cgaggagatc ggctggcgcg
     3721 gtttcctggt accggaattg gcgaagtcga tgacattctc caaggtggta ttgatcagcg
     3781 gagtcatttg gggggcgtac cacctgcctc tgatcttgtt aggtacctac cacaacaact
     3841 cttcgaacgc gctgccgttt gcggggcaac tcgtgatgtt cttcacagtg atcatgtcag
     3901 cctcggtgat cattgcttac ctgaggctca agtcgggcag tttgtggagc gctgcggtat
     3961 ttcacgccag tcacaatctg ttcgtgcaga caatcttcaa tcccatcacg atccagtacc
     4021 caaacacccc aaagtacgtc gacgagctgg gcctggtgct gccgctcacg atgcttcccg
     4081 tggcggtgta ttttttcgtc aagggacggc gggagttcgg cgtgatcgcg gttgggcaaa
     4141 gtatgcaggc cgtttgcccg gcgattatcg atgacgggtt cgagtccggt agccccagtt
     4201 ccattcgctg aaatcaagcc gggatcagtt cgcaattgaa catcccgcgc agcaaggaac
     4261 gatctccctg gtatcgaagc gcgggagcgg ccgaagcctg ggacgccgtc cttcaagttc
     4321 gctggcgtcg atctcgcccg gtcgctccag gcgcccatcg atgaccttgt agaccgtgcg
     4381 cgagaccttg cagtgcacgg tcagcggctg attcctgcct cgggcgagac ggtcgcgctt
     4441 gacctgacga cctcgtcttt gccaacgagt agctcggaag tgatctggtg gaggaagctc
     4501 accccgccac gggcgtatcc ctccgtctcg agcgtcgcgg cggggagcag ttttcctcct
     4561 tcgactcgaa agagcaggtc tttcgttgcc gagattccgc cactgccgct gatcgtggcc
     4621 gacacaccga cgtcgacaaa cctggcaccg gcgagcgtga atcggtccac ccgggcgtcc
     4681 atcacgatga agtttccgaa gtcttcggcg ctcagaagag tggccgtgac atcgtgccga
     4741 tcggacagtt tccaggcatt gtctgcgcgc cgcttcccga gggcgacgaa catctgatgc
     4801 gggaagtcgc tgtatttcgg ccggtcaacc gcttcgatcg cgtagacgac gacgctcgca
     4861 tcgccggcag cggccgtcac atattgaacg cacaccgcgg gctgcccgtt gatcgtgagc
     4921 ggccgcgatg gttgcacgat ccccgggtcg cacgggtagc gatgtacagc cgaaagctcg
     4981 atccgcgctg tcgccccgac ggtaaggagc gaagggaatc gcttggcgcg ccggctccat
     5041 caaactcgag cgacgatcga cgatggcgac atcgaatcat caaggaatga cgatgccggc
     5101 atccaggatg acgatttcga atgaaaaatg acatcgatga cttaagaaat gtcatcgatg
     5161 acattcggga tgagcttttc gcttaaaaca agtcatcgat gactaagaaa gtatcatcga
     5221 tgacctccag gatgcaaatt ttgcctaaaa cgagtcatcg atgactaaaa atatgtcatc
     5281 gatgacttcc aagatgccga tttcgcttaa gaaatgtcat cgatgacatc cagagaggac
     5341 gattttgaat caacaccgcc cacgtttaca tcgaaaatgc caacgccgag gcatcccata
     5401 ttcattgtca atcgtcctac ctctcacagt ctgtaacttc ggcccttgct gattcgtcta
     5461 agtaggaaag gcggcaattc atggcaagcg cagatgcgaa acgctgcatt gggaaaaacg
     5521 cgcgcagttt gcgtcgcgcg aagccgcgtt cgtgcgctgc ggagcgcgaa cgaggccggg
     5581 cgtggatgcg gccgttccgc cgcacttggc gcgccatcaa tcgctcgctg gcaggccggt
     5641 tcgacgacat ccccaccggg ttgttcgact ccgcggtgag cggcgccgtt ccaatccctg
     5701 cggatgcgca agcggcggca atgggcagag ccctcgcgct cttccggagg acccctacac
     5761 cactcgttcc gctcgatctc ctctctgccg aacgtcgcgt gccatgcatt cctggtcgcc
     5821 gacggcccgt tcgtgtagcc gtcgtcgaag caacaagacg gatcttcaga gggcgcgcgc
     5881 ctccgctcgc agcaacctgc tcgctctgat cacagcagtt gcgaaacgtg gaggctccga
     5941 tgtcctcatc ccatgaagta cccgtcacac ccccgcagcc gtcgtatggc gattccgcgc
     6001 tggcgctcgt cgaagagctc cgagccatga gagagaggat tccccatctc gtgatcccgg
     6061 agaccagaga cgcgcgccgc cgcctggcga atgccgcctc
//
LOCUS       DATLVW010000585         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_151640, whole genome shotgun sequence.
ACCESSION   DATLVW010000585 DATLVW010000000
VERSION     DATLVW010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151640"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VNN25_17260"
     CDS             complement(<1..1475)
                     /locus_tag="VNN25_17260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH93336.1"
                     /translation="MSNTPESGAGTGIRALRLGLATFAILALELAIIRWMSHQVRIFA
                     YLNNVLLMASFLGMGLGIAIGRRRPRLIDLALPSLVVLAAILTFSSQLHLLRIAFPDP
                     TLSIWGAETLRGNETFITNLAKIVALFALVVWIFVCAGSAVGPLFASLRPLQAYTADL
                     VGSLLGVVAMTAAAVLGTTPPLWLALAVIPLVVLSPRVLSIVSALAVVVLAQLSVQHA
                     LFSPYNRLDIGYSTVIPGHPLALAANRDAHQLMLDLSESNISNPRFTPEQQRFLATYR
                     YMYDLPFRVPAGRGRALVVGAGTGNDVAAALRNGFGQVVSVDIDPTIIAVGRRLHPEH
                     PYNDARAVPVVNDARAYFEQHPDDRFDIVCFGLLDSHAMFSSMSSLRLDNYVYTVASL
                     RSAWRHVAPGGALSVSFSVYAGEWIADRIFAVITEATGQRPRVFAFPIATAKTFIITK
                     GPSRMPDRPLILEVSQVTDLGATRVTTDDWPFLYLRPDSFPT"
     gene            1542..>2316
                     /locus_tag="VNN25_17265"
     CDS             1542..>2316
                     /locus_tag="VNN25_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93337.1"
                     /translation="MPFREDAQRFIDQKKFDDLESLWMSQLEKDPSDVDSFLVIARSL
                     RKAEQRTQSDTLLGLLSDTFLEKKAWPLRLQVLKELGRLSRHPATLRPAIEQALRGAH
                     GSHKNFQRVYDFAGFSDPTSNPVEKAEKIETWLRYDEGEMYFMAGRGAGIVTELNPEL
                     GIARLDFDKGERVSMPIGAAAKYLVPLPPGHVLREKFTDAEKLQAEAKKSPSQFFARI
                     LQSFGRPMQMAEVRDAVIGIVPEEKWSSWWTAARKNTQIV"
BASE COUNT          491 a          687 c          763 g          375 t
ORIGIN      
        1 gtgggaaagc tgtccggcct caggtagagg aagggccagt cgtcggtggt cacacgcgtg
       61 gcgccgaggt ccgtgacctg cgaaacctcg agaatcagag ggcggtccgg catccgggaa
      121 ggcccctttg taatgatgaa cgtcttcgcc gtggcgattg ggaacgcaaa aacgcgggga
      181 cgctggcccg tggcctccgt aatcaccgcg aagatccggt ctgcgatcca ctctcccgca
      241 taaacggaga acgagacgct caacgctccg ccgggagcta cgtgcctcca agccgagcgc
      301 aaggacgcaa cggtgtaaac gtagttgtcg agccgcaaag acgacatcga cgaaaacatg
      361 gcgtgcgagt cgagcagacc gaaacatacg atgtcgaagc ggtcgtccgg atgttgctcg
      421 aagtaggcgc gggcatcgtt gaccaccggc accgcacgag catcgttgta ggggtgttcg
      481 ggatgtaagc gtctgccgac cgcgatgatc gtcgggtcga tgtcgacgga gacgacctga
      541 ccgaaaccgt tgcgcagagc ggccgcgacg tcgttccccg ttcctgcccc caccacgaga
      601 gcccgccctc tccccgccgg cacgcggaac ggcaggtcgt acatataccg gtaggtagcc
      661 aggaagcgct gctgctctgg tgtgaatcga gggttggaaa tattcgactc gctcaggtcg
      721 agcatcagtt gatgggcgtc gcggttggcc gcaagcgcaa gcgggtggcc ggggatgacc
      781 gtcgaatagc cgatatcgag ccggttgtac ggcgagaaca acgcatgctg caccgagagt
      841 tgtgcgagga ccaccacggc gagcgcgctg acgatcgaaa gaacgcgcgg agacaggacg
      901 accagtggaa tcacggccag cgccagccag agtggcggcg tcgttccgag caccgcagca
      961 gcggtcatgg cgacgacgcc gagaagtgag ccaaccaggt cggcggtata agcttgcagt
     1021 ggacgcaagc tggcgaacaa cggtccgact gcgctcccgg cacagacgaa aatccagacc
     1081 accagagcga agagcgccac gatctttgcc aggttggtga tgaacgtctc gttgccgcgc
     1141 agtgtctccg caccccagat cgagagcgtc ggatcgggaa aggcgattcg caagagatgc
     1201 aattgagacg agaaggtcag gatcgccgca agcaccacca gcgacggcag cgccaggtcg
     1261 atcaggcgcg gccggcggcg gccgatggcg atgccgaggc ccatgccgag gaagctggcc
     1321 atgagcagta cgttgttcag gtaggcgaag atccgtacct ggtgagacat ccagcggatg
     1381 atggccagct cgagagcgag gatggcgaag gtggccagtc caaggcgtag cgcgcggatg
     1441 ccggtgcctg cgcccgactc gggagtgttg ctcaagcgcg ggattctagc gtgtgctgca
     1501 ctacaatggc ccgctctatt ccgcccggag ggatttcgac tttgccgttc cgcgaagatg
     1561 cccagcgttt catcgatcag aaaaaattcg acgatctcga gtctctttgg atgtcgcagc
     1621 tcgaaaaaga tccgagcgac gtcgactcat tcctcgtcat cgccagatcg cttcgcaagg
     1681 cggagcagcg cacgcaatcc gacacactcc tcgggcttct ctccgatacg tttctcgaga
     1741 agaaggcctg gccgctgcgc cttcaggtgc tgaaggagct gggccggctt tcgcggcatc
     1801 cggccacgct gcgtccggcg atcgaacagg cgcttcgggg cgcgcacggc tcgcacaaga
     1861 actttcagcg ggtctacgac ttcgctggtt tctccgatcc gaccagcaac ccggtcgaga
     1921 aggcggagaa gatcgagacc tggctgcggt acgacgaagg cgagatgtac ttcatggccg
     1981 gccgcggcgc tggcatcgtc acggagctga atcccgagct cggcatcgcc agactcgatt
     2041 tcgacaaggg cgagcgtgta tcgatgccca tcggagcagc ggcgaaatat ctcgttcctc
     2101 tgccgccggg ccacgttctg cgcgagaagt tcaccgacgc cgagaaattg caggccgagg
     2161 cgaagaagtc accgtcgcag ttcttcgccc gaatcctcca gagcttcggc cggccgatgc
     2221 agatggccga agtacgcgat gccgtgatcg gcatcgtccc cgaagagaag tggagctcct
     2281 ggtggaccgc ggcccgcaag aatacgcaga tcgtca
//
LOCUS       DATLVW010000586         3804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_395430, whole genome shotgun sequence.
ACCESSION   DATLVW010000586 DATLVW010000000
VERSION     DATLVW010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395430"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VNN25_17270"
     CDS             complement(<1..403)
                     /locus_tag="VNN25_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93338.1"
                     /translation="MTRLLPDPELDQLVRQLNEGEPAAADEPRGTWPAAPDASKGTWP
                     AAQVPSGNPLEALLLDAARRGASDLHIIAGSVPVFRVGGRLVRSEGSVLSDDDVAPLL
                     DPLLTPRVRERIAEDGAIDFSLRLDAPAVSEE"
     gene            486..2183
                     /locus_tag="VNN25_17275"
     CDS             486..2183
                     /locus_tag="VNN25_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH93339.1"
                     /translation="MRKLFAMVIIACLLPALSFADSTSFRSMQSGWFSGSTPLFAHGI
                     LGQRQIIAILDTGLDWDSCYFAEANGARPPMNTGSPSGGLSSSNVDTSRRKVIAYDFL
                     YSCDQFPGAFGCDDPNDPTAFDNALHGTLAAGVAAGDSHAYLQADDEADGIAPGAKLI
                     VQDGGYIGGDNCSQRPGFGCPVNLTPILDQAYKQGARIHSNSWGDRQSTPPSLPAPTA
                     NYSAAARDVDAFVWSHPDMLLVFDTGNQANNSPPPASSVPAPGCAKNGIQVGGTRYTG
                     IHPWDDVVTPFSYIGPTRDGRIKPDLVAPSYVVSAGSDGNVTTGNCNTSPQSGTSWSA
                     PSVAGAAALVRQYFTDGFYPTGVTTPANAFVPSAALLKAMLIAAARSVPNDWTYNVGL
                     TPAKPAPTFEQGFGFPVLDDAIYFPGDQARVRVADVPLAEGLTQGDTATLQLHVAAGT
                     PLKVALVWTDPAGIPRSVSDSTPELVNDLDLRVTDSTGTTLLGNDSLHPGQPDRLNNV
                     EVVSMATPPAGLYTISVNASHLGSGPRQSYALVITGDLTDAIPPIANVSARTRSARH"
     gene            complement(2573..3451)
                     /locus_tag="VNN25_17280"
     CDS             complement(2573..3451)
                     /locus_tag="VNN25_17280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HXH93340.1"
                     /translation="MKTLFLVNARSGANRRRDLTALIRETWEHEHEIFPCGSKEELVE
                     VVHSAATRGVRIIYAVGGDGTVHEVAKRLVHTDLVLGILPAGSGNGLARHLCVPIEPR
                     ASLRACGVLRIETIDTATVNGTPFVNAMGVGFDAWVADAFATAGTRGMQTYVRAGLRG
                     LATYEAEEYEVTIDGEMLRRSAIVIAVANASQYGNHARIAPLASVQDGLLDVTLIESV
                     SLLRMPMLLARLFAGSLHRARGVTMFRGRTITIKRSSAGPAHLDGEPVTMPESLTIAI
                     VPRSLKVIVPRAARAI"
     gene            complement(3448..>3804)
                     /locus_tag="VNN25_17285"
     CDS             complement(3448..>3804)
                     /locus_tag="VNN25_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93341.1"
                     /translation="VISHWVLDFLTHRPDLPLWPGGPKVGLGLWNMPRVEIVTEALLF
                     AIGIAVYRNVTKPRDAIGGAVFWLFVILLAGIFVANTAGTPPPDVRTLAWMALSMWIL
                     PLWAGWFDRHRYVVVT"
BASE COUNT          682 a         1256 c         1183 g          683 t
ORIGIN      
        1 gttcctcgct taccgccggc gcgtccagac gtagcgagaa atcgatcgcg ccgtcttcgg
       61 cgatgcgttc gcgtacccgc ggagtcagca gcgggtcgag aagcggggcg acgtcgtcgt
      121 cggagaggac ggagccttcg ctgcgcacga gccgtccacc gacacgaaag accggcaccg
      181 agccggcgat gatgtgcaga tcgctggcgc ctctgcgcgc cgcgtcgagt agaagtgctt
      241 cgagagggtt gccgctcggc acttgcgccg ccggccacgt tcccttcgac gcatccggcg
      301 ccgcaggcca cgtcccgcga ggttcatcgg ctgccgccgg ctcaccttca ttgagttggc
      361 ggacgagttg atcgagctca gggtcaggaa gaagtcttgt cataactgta gagtacgaat
      421 gaagtgtgcc agaagtagca gctcactcgg aatgccggcc catcctcgct tcgttgcacg
      481 cgtccatgcg gaagttattt gcgatggtaa tcatcgcatg cctgctgcct gcgctctcgt
      541 tcgccgactc aacctccttt cgatcgatgc agtcgggttg gttcagcggc tctactccct
      601 tgttcgcgca tggcatcctc ggccagcggc agatcatcgc catcctcgac accggtctcg
      661 attgggacag ttgctacttt gcggaagcga acggcgcgcg tccacccatg aacacgggat
      721 cgccgtcggg cggtttgagt tcgtcgaacg tcgacacgag ccggcgcaaa gtcatcgcct
      781 acgattttct ctattcgtgc gatcagttcc ccggcgcctt cggctgcgat gatccgaacg
      841 atcccactgc cttcgacaat gcgcttcacg gcacgctcgc cgcgggggtc gctgccggcg
      901 acagccacgc gtatctccag gcggatgatg aagcggatgg catcgcgccg ggtgcgaaac
      961 tgatcgtgca ggacggcggc tacatcggcg gggacaactg cagtcagcgg cccggcttcg
     1021 gatgcccggt caacctcaca ccgattctcg atcaggccta caaacaaggc gcgcgtattc
     1081 actccaactc gtggggcgac cgccagagca cgccgccgtc tcttcccgct cctacggcga
     1141 actactccgc cgccgcgcgc gacgtcgatg cgttcgtctg gtcgcatccc gatatgctcc
     1201 ttgtcttcga taccggcaac caggcaaaca attcgccgcc gcccgccagc tcggttccgg
     1261 caccgggctg cgcgaagaac gggattcagg tcggcggaac gcgatacacc ggcattcatc
     1321 cctgggacga tgtcgtcacg cccttctcct acatcggtcc gacgcgcgat ggacgaatca
     1381 agcctgatct cgtcgcgccg tcgtatgttg tttccgcggg gagcgatggc aatgtgacga
     1441 ccggcaactg caatacgagt cctcagtcgg gcacatcgtg gtcggcaccg tccgtcgcag
     1501 gagcagcagc gctcgtgcgg cagtatttca ccgatggttt ctatccaacc ggcgtcacca
     1561 cgccggcaaa tgcattcgtt ccgagcgccg cgctgctgaa agcgatgctg attgccgccg
     1621 ctcgctcggt gccgaacgac tggacgtaca acgtcggtct cacgccggcg aagcctgcgc
     1681 cgacattcga acaagggttc ggctttccgg tactcgacga cgcgatctac tttcccggcg
     1741 atcaagcgcg cgtgcgcgtc gccgacgttc cgctcgccga ggggctgacg cagggcgata
     1801 ccgccacgct gcagctccat gtcgccgcag gcactccgtt gaaagtcgcg ctggtctgga
     1861 ccgatcctgc tggaatcccg cgcagcgtgt cggattcaac gcctgagctg gtgaacgatc
     1921 tcgacctccg cgttaccgat agcaccggca ccacgctcct cggcaacgac tcgctccatc
     1981 ccggccagcc cgatcgcttg aacaacgtcg aagtcgtatc gatggcaact ccgcccgcag
     2041 gcctctacac gatctccgtc aacgcctcac acctcggcag cggaccacgc cagagctacg
     2101 ccctcgtcat caccggcgac ctgaccgacg cgatcccgcc gatcgcgaac gtaagtgcgc
     2161 gaacacgatc cgcgcggcac tgaccaagcc ctcatttctc aactttttca gcgactctct
     2221 tgcgtagagc gccttctccc tttggcaacg tttgtagcga aggcaaatgc cccctcatcc
     2281 ggccttcggc caccttctcc ccctctgaaa agcgaggggg agaaggcgct cgtgggagag
     2341 gctgtggcga agggtggtgg caggtggtgg cggtcgcgcg gtcaggcggt cgcgcggtca
     2401 ggcggtggcg gttatgcaat gctcatgaat cgagcgcctt ctcccctcgt gcttttcgag
     2461 gggagaaggt gccgaaggcg gaggggtgtc gtgcaatcgc gtgacatgga gtttccggcg
     2521 cgcaagagtg ggcgttcgat gaaaaggtcg cgaagagtgt aagaaaagcg gctcaaatcg
     2581 cccgtgccgc cctcggcacg atcaccttca gcgagcgcgg cacgatcgcg atcgtcagcg
     2641 actccggcat cgttaccggc tcgccatcga ggtgcgctgg tccggctgac gaacgtttga
     2701 tcgtgatcgt gcggcctcgg aacatcgtta cgccgcgtgc gcgatgcagg cttccggcga
     2761 agagccgcgc gagaagcatc ggcattcgga gaagcgacac gctctcgatc agcgtcacgt
     2821 ccagcagccc gtcctgtact gacgccagcg gcgcaatgcg tgcgtggttg ccgtactggc
     2881 tggcgttggc gacggcgatg acgatggcgc ttcgccgcag catctctcca tcgatcgtca
     2941 cctcgtactc ctcggcttcg taggttgcga gtccacgaag gccggcgcgc acgtaggtct
     3001 gcatgccgcg cgttcccgca gtggcgaagg catccgcaac ccaggcgtcg aagccgacac
     3061 ccatcgcgtt gacgaatggc gtgccattca ccgtcgccgt atcgatggtc tcgatgcgaa
     3121 ggacaccgca tgcgcgcagc gatgcccgcg gctcgatcgg tacgcagagg tgtcgggcca
     3181 gaccgttgcc ggaaccggcg ggaaggatgc cgagaacgag gtcggtatgg acgagccgct
     3241 tcgcgacttc atggaccgtc ccgtcgccac cgaccgcgta gatgatgcgc acgccgcgcg
     3301 ttgccgcgga gtggaccacc tcaacgagct cctctttcga tccgcacggg aagatctcgt
     3361 gctcgtgctc ccacgtttcg cgaatcagag cggtcaggtc gcgccgccgg tttgcgccgg
     3421 agcgcgcgtt gaccaggaag agggtcttca cgtgacgacg acgtagcgat gccggtcgaa
     3481 ccagccggcc cacagcggca ggatccacat cgacagcgcc atccacgcca gcgtacgcac
     3541 atcaggcggc ggtgttccgg ccgtgttggc gacgaagatg ccggcgagca ggatcacgaa
     3601 gagccagaag acggcgccgc cgatcgcgtc gcgcggcttc gtgacgtttc gatacaccgc
     3661 gatcccgatc gcaaacaaca acgcctccgt cacgatctcc acgcgcggca tgttccaaag
     3721 cccgagtccg actttcggtc caccgggcca gagcggcagg tcgggacgat gcgtgaggaa
     3781 atcgagcacc cagtgactga tcac
//
LOCUS       DATLVW010000587         4254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_108074, whole genome shotgun sequence.
ACCESSION   DATLVW010000587 DATLVW010000000
VERSION     DATLVW010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108074"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..710
                     /locus_tag="VNN25_17290"
     CDS             150..710
                     /locus_tag="VNN25_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXH93342.1"
                     /translation="MIPGGEITQLLSAASAGDRDALDRVFDLVYRELRSIARGQLASG
                     ETLRTTALVHEVYLRMAQGQAISARDREHFYATAARAMRQILVDHARRRAAGKRGGGV
                     RPEVLDDQNVGLETREEEILAIDTALSRLEDVDIRLAKLVEMRFYGGLSVAETAETLG
                     LSERTVKRDWRKARAFLYHELHGSAA"
     gene            710..3523
                     /locus_tag="VNN25_17295"
     CDS             710..3523
                     /locus_tag="VNN25_17295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HXH93343.1"
                     /translation="MTTIPPDSQRWQRINALLDEGLDLPLAGREGWLDRVCGDDRALR
                     RELEELLDADAASTDFLDEASVILGDILRDAALDRADRRAAAHIGQRAGAFRLVREIG
                     RGGMGSVYLAERADGQFEQRVAVKLLEGDDARLLPFFLRERQILARLEHPNIARLIDG
                     GLTDDDVPYLVMEYVEGEPITEYCNRRTLAIPERLRIFQSVCAAVEAAHRMHVVHRDL
                     KPSNILVTSDGQVKLLDFGIARRIDLGAEVTRTLAPALTPAYAAPEQLRGEMPAPATD
                     IYALGLLLYELLTGIRAQQPKGEGIDAILYAVLKEEPSPPSSMSHRPELRGDLDRITM
                     KSIEKDPSRRYPTAAALSGDIERHLAGEPITASGGFTYRAAKLLRRRRVEAGTVAIAI
                     IATILALILWRSGGQTTQSLQFELFSTFGGSPRQPALSPDGTRVTYVQPDGAGVPQLF
                     VRPLANGDAVQLTRGGVVGARAPRWSADGTIFYDVPGDGIWKIPAAGGTPHRIVARGF
                     HASLSSDGKLLLYQQAALLYLAHADGSGARVVTGVRLHFAFGQAGAALSPDGELIAYA
                     QDDRSPVNTDLYVVPVSGGPPRRLTFDNITVSEPAWAPDGKTIYFSSRREGSLTLWRI
                     AAAGGTPQQVTSGSGEDTESTISSDGRRLFYTNARAAFSLSWLDPVTGARRSLRDERT
                     PLTHPSFSPDEKRIVFFRSRGQREEIFSMAVDGTDERQLTTPAAGGNDVLPDYSFDGR
                     TIYFYRLAPNPAFVAMPAGGGAIRTVLPDFPLYEHVGVHVDPGGRRAVFPIMDSGTFR
                     STVVRDLTTRREQRLGMPLAWPRWSPDGKWIAGTSVPGGHLTLCAAEGGVCRTLSSKG
                     GDPRWSQGYVYFASYSEPLGEADVKPIEIRRVRPDGSGEELVATLPGADPIEFFFDVS
                     PSGKIVWCELRSSRSEIWTAKLP"
     gene            3581..4072
                     /locus_tag="VNN25_17300"
     CDS             3581..4072
                     /locus_tag="VNN25_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93344.1"
                     /translation="MKRSALITIVLALALALPVATFAGQGENSPATGTLVGSWVASVT
                     PDPASGVPPFRELFTFHNDGTLTMTDDNAPAPPFPFTPGHGVWKKSDQVNYVFHYLNL
                     IYDPASYASNGQTSITVHVKLGDDRDSFSGTGHLKVSDNAGNVLFEADGTISATRITI
                     DQP"
BASE COUNT          696 a         1435 c         1366 g          757 t
ORIGIN      
        1 cctccgtcgt cgcaattgac atcgtcgcgc acacaggtgc ctttctcggc agaggcgcac
       61 ccgcgatgtc gaccagcggt tcacgcgcgg acgtcctctg ccacatcgca catccgccgc
      121 gctagaatcg gcgtaaatca agcaggaccg tgatacccgg cggcgaaatt acgcagctcc
      181 tttcggcagc gagtgcaggc gaccgcgatg cgctcgatcg cgtcttcgac ctcgtctacc
      241 gggagctgcg ctcgatcgcg cgaggccagc tcgccagcgg cgagacgctg cgcacgacgg
      301 ctctcgtcca tgaggtctac cttcgcatgg cccagggaca ggccatcagc gcgcgcgatc
      361 gtgagcattt ctatgccacg gctgcaagag ccatgcggca gatcctcgtc gaccatgcgc
      421 ggcgccgtgc ggccggtaaa cgcggtggcg gtgtgcggcc tgaagtgctc gacgatcaga
      481 acgtcgggtt ggagacgcgc gaagaggaga tcctggcgat cgacacggcg ctcagtcgcc
      541 tcgaagacgt cgacattcgt ctggccaaac tcgtcgagat gcgtttctac ggcggccttt
      601 ccgtcgccga gaccgccgag actctcggcc tatcggagcg gaccgtgaag cgagactggc
      661 gcaaggcacg cgcgtttctc tatcacgagt tgcatggcag cgccgcctga tgacgacgat
      721 cccgccagat tcgcagcggt ggcagcggat caacgccctg ctcgatgagg ggctcgatct
      781 gccgcttgcc gggcgtgagg gatggctcga ccgcgtctgc ggcgacgacc gtgccctacg
      841 ccgcgagctc gaggaactgc tggacgccga tgcggcgagc accgactttc tcgatgaggc
      901 gagcgtcatt ctgggcgaca ttcttcgcga tgcggcgctt gaccgcgccg atcgccgcgc
      961 cgcggcgcac atcggacagc gcgccggagc attccggctc gtccgcgaga ttggccgcgg
     1021 cggcatggga agcgtctatc tcgccgagcg cgccgatggc cagttcgagc agcgcgtagc
     1081 ggttaagctc ctcgaagggg atgacgcccg gctgctccct ttctttctgc gggagcggca
     1141 gatcctggcg cggctcgagc atcccaatat cgcccgcctg atcgacggcg gtctcaccga
     1201 cgacgatgtc ccgtacctcg tgatggagta cgtcgaggga gagccgatca ccgaatattg
     1261 caaccgccga acgctggcga ttccggaacg gctgcggatc tttcagtccg tctgcgcggc
     1321 ggtagaagcg gcgcaccgca tgcacgtcgt ccaccgcgat ctcaagccgt cgaacattct
     1381 cgtgaccagt gacggacagg tgaagctgct cgacttcggt atcgcccggc gcatcgatct
     1441 tggcgcggag gtcacgcgga cgctggcgcc tgcgctcacg cctgcttatg cagcccccga
     1501 acagcttcgc ggcgagatgc cggcgccggc cacggacatc tacgcactcg gacttcttct
     1561 gtacgagctg ctcaccggga tccgggcgca acaaccgaaa ggggagggca tagacgccat
     1621 tctctacgcg gtgctcaagg aagaaccttc gccgccgagc tccatgtcgc accgcccgga
     1681 gcttcgtgga gacctcgacc ggatcacgat gaagtcgatc gaaaaggatc cgtcgcggcg
     1741 ctatccgacg gctgccgcgc tttccggaga catcgagcgc catctggccg gcgagccgat
     1801 caccgcgtcg ggcggcttca cgtatcgcgc ggcaaagctc ctgcgacggc ggcgtgtcga
     1861 ggccgggacg gttgccattg cgatcatcgc cacgattctt gcgctcatcc tctggcggag
     1921 tggcgggcag acgacgcaat cgctccagtt cgagctcttc tcgacgttcg gcggctcgcc
     1981 gcgacagccg gcgttgtctc ccgacggcac cagggtgacc tacgtccagc cggatggcgc
     2041 gggcgttccg cagctcttcg tccggcccct cgccaacggt gatgccgtcc aactcacgcg
     2101 tggaggcgtc gttggcgccc gcgcgccgcg ctggtcggcc gacggcacaa tcttctacga
     2161 cgtgccgggc gacgggatct ggaaaatccc tgccgcggga ggtacgccac accggatcgt
     2221 cgcccgagga tttcatgcga gtctttcgag cgatgggaag ttgctgctct accagcaggc
     2281 tgcgcttctg tacctggctc acgcggacgg cagcggtgcc cgggtcgtca cgggcgtccg
     2341 gttgcatttc gcattcggcc aggccggcgc ggctctctcg cctgacggcg agctgatcgc
     2401 gtatgcccag gatgatcgct cgccggtcaa caccgatctc tacgtcgttc cggtgagcgg
     2461 cggtccgcca cgccggttga cgttcgacaa cattaccgtc agtgagccgg cctgggcgcc
     2521 ggatgggaag acgatctatt tctcttcgcg gcgagaaggt tccctcacgc tgtggcgcat
     2581 cgcggccgcg ggcggcacgc cgcagcaggt caccagcggc tcaggggagg acacggaaag
     2641 caccatctcc tccgacggcc ggcgcctctt ctacaccaac gctcgcgcgg cattctcgct
     2701 gagctggctc gatccggtca ccggtgcacg ccggtctctg cgcgatgagc ggacgccgct
     2761 gacgcacccg agcttctcgc cggatgagaa gcgcatcgtc ttctttcgca gccgcggcca
     2821 gcgcgaggag atcttcagca tggctgtcga cggtacggac gagcgccagc tcacgacgcc
     2881 ggcggccggc ggcaacgacg tacttcccga ctactcgttc gacggccgga cgatctactt
     2941 ctatcgtctg gcgccgaacc ccgccttcgt cgccatgccg gcgggtgggg gagcgatccg
     3001 tacggttctt ccggactttc ctctttacga gcacgtcgga gtgcacgtcg atcccggggg
     3061 tcgtcgcgcc gtgtttccga tcatggattc cggcaccttc cgctcgacgg tcgtccgtga
     3121 cctcaccact cgtcgcgagc aacggctcgg gatgcccctg gcatggccgc ggtggtcgcc
     3181 ggacggaaag tggattgccg ggacgtcggt tcccggcggt cacctgacgc tttgcgcggc
     3241 cgaaggcggt gtctgccgca ccctgtcgtc caaagggggc gatccccggt ggtcgcaggg
     3301 atacgtctac ttcgccagct acagcgagcc gctcggcgaa gcggacgtca agccgatcga
     3361 gatccgccgc gttcgtcccg atggcagcgg cgaagagctc gtggccacgc ttccaggcgc
     3421 cgatccaatc gagtttttct tcgacgtctc gccgagcggg aagatcgtct ggtgcgaact
     3481 tcggtcgagc cggtcggaaa tctggacggc caaactcccc tgatgtcccc ttttggtctc
     3541 cttttgcgtg gtcctgggca gaaaggaaaa ggagatcacc atgaaacgct ctgcactcat
     3601 cacgattgtt ctcgcccttg ccctcgccct tcccgtcgcc acgttcgccg ggcagggaga
     3661 gaattcccct gcgaccggca ccctcgttgg gagctgggtt gcctcggtta cccccgatcc
     3721 tgccagcgga gtcccgccgt tccgtgagct cttcacgttc cacaacgacg gtaccctgac
     3781 gatgaccgac gacaacgctc ccgcgccacc gttccccttc actcccggtc acggtgtctg
     3841 gaagaagagc gatcaggtca actatgtgtt ccactacctg aacctgatct atgacccggc
     3901 gagctacgcc tcgaacggcc agacgagcat caccgtccac gtcaagttag gcgacgacag
     3961 agacagcttt agcggcaccg gccacctgaa ggtttccgat aacgccggca acgtgctgtt
     4021 cgaagcggac ggcacgatca gcgccacccg gatcacgatc gaccagccgt gagctaggtc
     4081 gcaggtcgcg ggtcgcgggt cgccgggaag gcggcccgcg tcgcgctccg ctgctcagcc
     4141 gattcgatga cgcctggccg attcctccgc cgggcacgaa ctgcagcgat gtttctggac
     4201 gttgacgtgg cgaggggcgg ttttcaggcg cggcgcccac acttcgtccg tccg
//
LOCUS       DATLVW010000588         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_69642, whole genome shotgun sequence.
ACCESSION   DATLVW010000588 DATLVW010000000
VERSION     DATLVW010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69642"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VNN25_17305"
     CDS             <1..1147
                     /locus_tag="VNN25_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH93345.1"
                     /translation="TEVIESARRMVLQFVDVDESTHDVCFVANTSAAIKLVAESYPFS
                     PESNLILSVDNHNSVNGIREYARRAGARVRYLPLDDELRLLDAELFLCEESLRGGGLV
                     AFPAQSNFSGVQHPLDLVMKARALGFDVLLDVAAFVPSHAFSLRRCPADFVALSFYKL
                     FGYPTGIGALVARRDALDRLRRPWFAGGTVLYASVAADEHRLRPHHDAFEDGTPNFLG
                     IAALDAGFELLAEVGMPRLTAHVAQLTGDLLDDLRALRHGNGAPLVRIYGPRDATDRG
                     GAVAFNVCDREGTPIAYSLVEARARRAGVSLRGGCFCNPGASEAAFGLDAPRIAACFD
                     SLGAGFTPERFAACSNTAVGAVRASIGLANNSDDIRRAVGVVASFRE"
     gene            1176..1826
                     /locus_tag="VNN25_17310"
     CDS             1176..1826
                     /locus_tag="VNN25_17310"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HXH93346.1"
                     /translation="MAQIRFRFAPEKLVQALVFFAQHGVRDLDKMKAAKLLFHADKYH
                     LLQYGRPVIGDQYACMEYGPVPSASLNVMNDVVAHDKHFKPLAKDLFDEYLTVEKSMF
                     RKYPVFRARKDPDLDVFSESDVEALEYAQKNYGSTPAWKLSEQSHEEPSWKLANEQRA
                     PGSSVIMDYRLFFEGHPEAADMLRFVEAQQEDRDAAEELAMGRAVQPERHLAGAGR"
     gene            complement(1823..2248)
                     /locus_tag="VNN25_17315"
     CDS             complement(1823..2248)
                     /locus_tag="VNN25_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93347.1"
                     /translation="MSFEDDLADAEGRARGAVFHHFSNRAGKTKFLVALNHIWPPDDG
                     LVVYAFTTTNVDHFAGERARHLPVVRLNPGDYAFVTSPTVIDLTRPEIEQLAAIVSAP
                     SFKFVVQLRPEHVRAIDEAVRASTVIVRKLKRLILANYG"
     gene            complement(2253..>2400)
                     /locus_tag="VNN25_17320"
     CDS             complement(2253..>2400)
                     /locus_tag="VNN25_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012666766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HXH93348.1"
                     /translation="DGCYVNYPDSDLKNWEYLYYLDNYPALQKVKARWDPNNIFNHEQ
                     SVRK"
BASE COUNT          409 a          780 c          730 g          481 t
ORIGIN      
        1 cactgaggtc atcgagtcgg cgcggaggat ggtgctgcaa ttcgtcgacg tcgacgaatc
       61 gacgcacgac gtctgcttcg tcgccaacac cagcgcggcc atcaagctcg tcgcggagtc
      121 gtatccgttc tcgccggaat cgaacctcat cctctcggtc gacaatcaca actccgtgaa
      181 tgggatccgc gaatacgcgc ggcgtgccgg cgcgcgcgtc cgctatcttc cgctcgacga
      241 cgagctgcgc ctgctcgatg cggagctgtt tctatgcgaa gagtcgctgc ggggcggcgg
      301 ccttgtggcc ttccccgcgc aatcgaattt ctccggcgtg cagcatccgc tcgatctggt
      361 gatgaaagcg cgcgccctcg gcttcgacgt tctgctcgac gtggccgcct tcgtccccag
      421 ccacgccttc agtcttcgcc gatgcccggc cgacttcgtg gcgctgtcgt tctacaaact
      481 cttcggctat ccgaccggca tcggcgccct ggtggcgcgg cgcgatgcgc tggaccgtct
      541 ccgccgcccc tggttcgccg gcgggacggt gctgtacgcg tcggtggctg ccgatgagca
      601 tcgtctccgc cctcaccacg atgccttcga agacggcacc cccaacttcc tcggcatcgc
      661 cgcgctcgac gccggctttg agttgctggc cgaggtgggg atgccacgcc tgaccgcaca
      721 cgtcgcgcaa ctgactggtg atctcctcga tgatctccgc gctctccgcc acggcaacgg
      781 cgctccgctc gtccgcatct acggcccgcg cgatgctacc gaccgaggag gagccgtcgc
      841 cttcaacgtt tgcgaccgcg agggaacgcc gatcgcgtac tcgctcgtcg aagcgcgcgc
      901 ccgtcgtgcc ggtgtctcgc ttcgcggcgg ttgcttctgt aaccccggcg cctcggaggc
      961 ggccttcggc ctcgacgcgc cgcgcatcgc cgcctgcttc gattccctcg gcgccggctt
     1021 cacccccgaa cgcttcgccg cctgcagcaa caccgccgtc ggcgccgtgc gtgcgtcgat
     1081 cggtctggcc aacaattccg acgacatccg gcgggcggtg ggggtggtgg cgtcgtttcg
     1141 ggagtgagtc cgaggtacac tttcatcgga caaccatggc gcagattcga tttcgcttcg
     1201 ctcctgagaa attggttcag gctcttgtct ttttcgcgca gcacggtgtt cgcgatcttg
     1261 acaagatgaa ggcggcgaag cttttgtttc acgccgacaa ataccatctg ctgcaatacg
     1321 gccgcccggt catcggcgat cagtacgcgt gcatggagta cggcccggtt ccttccgcgt
     1381 cgctcaacgt gatgaacgat gtcgtcgctc acgacaaaca tttcaagccg ttggcgaagg
     1441 atctcttcga cgagtatctg accgtcgaaa aatcgatgtt tcggaagtat ccagtcttcc
     1501 gcgccaggaa agatccggat ctggacgtct tctccgagtc cgatgtcgag gcgctcgagt
     1561 acgcgcagaa gaattacgga tcaaccccgg cttggaaact gagcgaacag tcgcacgagg
     1621 agccctcctg gaaacttgcc aacgaacagc gcgctccggg aagcagcgtg atcatggact
     1681 accggctctt cttcgaagga catccggagg ccgcggacat gcttcgcttc gtcgaagcgc
     1741 agcaggaaga tcgcgatgcc gccgaggagt tggcgatggg cagggccgtt cagccggagc
     1801 ggcacctggc gggtgcggga cgctaaccgt agttggccag gatcaacctc ttcaacttcc
     1861 gaacgatcac cgtcgatgcg cgaacagcct catcgatcgc cctgacgtgt tccggtcgaa
     1921 gttgcaccac aaacttgaag gaaggcgcgc tcacgatcgc cgcgagctgc tcgatctcgg
     1981 gtctggtcag gtctatgact gtcggcgacg tgacgaatgc gtaatctcca gggttcaacc
     2041 gcacgaccgg caggtgccgt gcccgttctc ccgcgaagtg gtcaacgttc gtggtcgtga
     2101 acgcgtagac gaccagcccg tcatcgggcg gccagatgtg gttgagcgcg acgaggaact
     2161 tcgtcttgcc cgcccggttg gaaaagtgat gaaacaccgc tccgcgagcc cggccttcgg
     2221 cgtcggcgag atcatcttca aacgacaccc ggctatttcc gtaccgactg ctcgtgattg
     2281 aaaatgttgt tcggatccca ccgagccttc accttctgca gtgccgggta gttgtcgagg
     2341 tagtagaggt actcccaatt cttcaggtcg gagtcgggat agttgacgta gcagccgtcc
//
LOCUS       DATLVW010000589         5038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_561304, whole genome shotgun sequence.
ACCESSION   DATLVW010000589 DATLVW010000000
VERSION     DATLVW010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561304"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..266
                     /locus_tag="VNN25_17325"
     CDS             39..266
                     /locus_tag="VNN25_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93349.1"
                     /translation="MPAPSRNANLQLEKLVGRQSLHVSVPRDVTDKDFGRLGKSIIDV
                     IKGHTGCSCLSGVIDVIVTGDFTNAINVDLG"
     gene            273..1811
                     /locus_tag="VNN25_17330"
     CDS             273..1811
                     /locus_tag="VNN25_17330"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HXH93350.1"
                     /translation="MSNLNNGHDHRATPTVVRPMVGLLNNPAIPGIISAAGSLVEYVE
                     VIPDRLWFDFGQRGRGGRFHKTHATVETLRELARTRKLTGHGIGLSLPSAMPLDEEML
                     SEIASLNRQLDFQWYSEHLSMFLVPHGSVPNAQAGLGLPIAYDDEMFALVREKLARLR
                     QVLDCPLLMENPSVFTPIDDAEMSEPEFLNRLWDEERCGTLLDLHNLYVSWRNGGMDP
                     RKYLAQLNPDAVVEIHMAGGDEIAGFYTDSHSRLSPPEVWEYAYEIVPRCHRLRGITF
                     EFHESYFPRLGLAGILGELEAMHELAACAGVDSGVVVRESGVDSGVAARVSGVGDEPG
                     REFEGTRDDAISLPHPTPHTPPPNPDFQQALADLVSSPAFCTEARRDPAVLRDRYQLT
                     DRESRRLEQIVNHPGMACNCMLYRANRLAPLALNLPELVKALGPDLREMLDAFWTEYT
                     NTDVHFYVESYRFCEFIEREIARGRTFTAEVAPALEREMSILADRLEVSHTEIYSPLR
                     EQRA"
     gene            1913..1988
                     /locus_tag="VNN25_17335"
     tRNA            1913..1988
                     /locus_tag="VNN25_17335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1946..1948,aa:Val,seq:cac)
     gene            2147..4216
                     /locus_tag="VNN25_17340"
     CDS             2147..4216
                     /locus_tag="VNN25_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93351.1"
                     /translation="MNARTRACFLAVLILCAFRVFADEPAIIKPMFGLNYTLGPVRNS
                     SGWMAARIPITIEKDAKKTIAIETTYVTTTASVNASWAQCVRTGFSRPPGTTLAILIT
                     STCANLGPATYEVNVAVTSEKQVQRLVFQITRPVGELRPLTTQIVDRTDSIACIEIAP
                     KELIVVETGARTPLTNVSASRIENLAIDGKAIDGDLKFGNANADSRGVARLPVTLSGH
                     FPNGVAKTTVMIRANELAAPVFVTYEIHARFHPWLLFLAILIGLLLGYAMRTLLVRFV
                     ESRTAYIAALDLRDKIDSEKKKYSDPTFIGIVDPLLTRLRDAIDKRPVDLTAIVNDIK
                     EKYNAAVSTLQDKIKAVRAAFDASVSLLSPDDFPPDIAKALTEAQQTIDEARKLVDSD
                     PTGAQNELTQATANLQADVDSAAAGWRVEMQVALADAVRSPLPPNFDEPFRSALATLK
                     TGLEQLPIGQSKDTVTILSAVHGLQFTLRFNLVKRVLDPMQTYGQSVIDVLKQKSEPV
                     AGSEAALQAFAAANGPAPLAQLDALLSALAADIKAAGDKNGVNVQNDLAAGKYLEAAR
                     AAVKQPPIAAIGSLPGTLATPLAAAGEAFPVSGLQFVQRAFPHAQLALPDTDTIERQR
                     SVWEIAAAKLARFAISALGISMAGLLVLLPAFDGTWRGLLTGLFWGYAGDISVDALTE
                     AAKKYRT"
     gene            complement(4219..>5038)
                     /locus_tag="VNN25_17345"
     CDS             complement(4219..>5038)
                     /locus_tag="VNN25_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93352.1"
                     /translation="SARAVETMEIINGETKSIKQMPTFFPRQIELVNELTRATVNVEV
                     RDLDAKSELHKTIPVWLVPRTTAPLSVLDPSTGQWRDLTAYFAAFVTPNAPAVMEFVR
                     NVTDAHPKKELVGYQIADTRVQPQVQATFDALKALGIRYVQSVIQFTRESGAANQRVR
                     RPAETLKDKNANCIDSTLLMASLLEAISLNPAIVLIPGHAFLAWETWRDGNGGSDQWK
                     YVETTMIGTATFEDACAEGEKKAADWQASAKGNPDYFRRLALSDLRAQGITPLE"
BASE COUNT          957 a         1658 c         1492 g          931 t
ORIGIN      
        1 ccttgtggac gtaaacctca aacaaaggag aacaccacat gcccgctccc tctcggaatg
       61 cgaatctcca actggaaaaa ctcgtcggcc gtcaatcgct tcatgtcagc gtcccgcgcg
      121 atgtcaccga caaagacttc ggccgcctcg gcaagtcgat catcgacgtg atcaagggcc
      181 acaccggctg cagttgcctc tccggcgtca tcgacgtcat cgtcacgggc gacttcacca
      241 acgccatcaa cgtcgatctg gggtagaccg gcgtgtccaa cctgaacaat ggccatgacc
      301 accgtgccac tccaacggtg gtcaggccga tggtcggact tctgaacaat cctgcgatcc
      361 cggggatcat ctctgcggcc ggcagccttg tcgagtacgt cgaggtgatc cccgatcgtc
      421 tctggtttga tttcggacag cgcggccgcg gcggccgctt tcacaagacg cacgccacgg
      481 tcgagacgct gcgcgaactg gcgcggacga ggaagctcac cggacacggc atcggcctct
      541 ccctgccgag cgcgatgccg ctcgatgaag agatgctctc cgagatcgcg tcgctgaacc
      601 gccagctcga tttccagtgg tacagcgagc acctcagcat gttcctcgtc ccgcacggca
      661 gcgtccccaa cgcccaggcc ggactgggcc tgcccatcgc ctacgacgac gagatgttcg
      721 ccctcgttcg cgaaaaactg gcccggctgc gccaggtcct cgactgtccg ctgctgatgg
      781 aaaacccgag cgtcttcacg ccgatcgacg acgccgagat gagcgagccc gaattcctca
      841 atcgcctatg ggatgaagaa cgctgcggga ctctgctcga tctgcacaat ctctatgtga
      901 gctggcgcaa cggcgggatg gacccacgca agtacctcgc gcagctcaat cccgatgccg
      961 tggtcgagat tcacatggcc ggcggcgacg agatcgccgg tttctacacc gactcgcact
     1021 cgcgcctgag cccgcccgaa gtgtgggagt acgcgtacga gatcgtcccg cgatgccacc
     1081 gcctgcgcgg catcaccttc gagttccacg aatcctactt cccccgcctc ggcctcgccg
     1141 gcatcctcgg cgagctggaa gcaatgcacg agctggcggc gtgcgcggga gtcgattcgg
     1201 gggtcgttgt tcgggagtcg ggggtcgatt cgggtgtcgc tgctcgggtg tcgggtgtcg
     1261 gagatgaacc gggccgcgaa ttcgaaggca cgcgtgacga cgcgatctct ctcccacacc
     1321 cgacccccca caccccaccc cccaaccccg acttccaaca agccctcgcc gacctcgtct
     1381 cgtcgccggc tttctgcacc gaggcgcggc gcgatcccgc ggtgctgcgc gacaggtatc
     1441 agttgaccga ccgcgaatcc cgccgcctcg agcagatcgt caaccacccg ggcatggcct
     1501 gcaactgcat gctctaccgc gccaaccgcc tcgccccgct ggccctcaac ctccccgaac
     1561 tcgtcaaagc cctcggcccc gacctgcgcg aaatgctcga cgccttctgg accgagtaca
     1621 ccaacaccga cgtccacttc tacgtcgagt cctaccgttt ctgcgagttc atcgagcgcg
     1681 agatcgcccg cggccggacg ttcactgccg aggtcgcacc agccctggag cgggagatgt
     1741 ccatcctcgc cgaccgcctg gaagtgagcc acaccgagat ctacagtcca ctgcgcgaac
     1801 agcgcgcctg atccgtcctc gatccttcgt tgacgcgcgc ggacactcgc actatcctgt
     1861 cccccctcgc tgaaatagcg aggaaacgca gctcgttgag acgatcccaa gcaggcgctt
     1921 agctcagttg gtagagcatc accttcacac ggtgggggtc accggttcga gtccggtagc
     1981 gcctaccaag tcgcaaacta ccgaaaagcc cgaggttctg cgcctcgggc ctttttgcgt
     2041 ttgggcggtt ttcagcctcc aactcttcat acaacagtac gtacgacgac gtagagcacg
     2101 gacgtgttga cgtgtttccg tgtcgcgggc acaatcgaca actccaatga atgcccgaac
     2161 gcgtgcgtgc tttctcgccg tgctgattct gtgcgccttc cgcgtcttcg ccgatgaacc
     2221 ggcgattatc aagccgatgt tcggtctcaa ctacacccta ggcccagtgc gcaacagcag
     2281 cggatggatg gcggcgcgca ttccaatcac tatcgagaaa gacgcgaaaa agaccatagc
     2341 gatcgagacc acgtatgtca cgacgaccgc ctcggtcaat gcatcgtggg cgcaatgcgt
     2401 tcgaacgggt ttcagccgcc cgcccggcac cacgctcgcg attctgatta cgtcgacgtg
     2461 cgcgaacctc gggccggcga cctacgaagt caacgtcgct gttacttccg aaaagcaggt
     2521 ccagagactg gtctttcaga ttacgcggcc ggtgggcgaa ttgcgtccat tgacgacgca
     2581 gatcgtggac cgaaccgatt cgatcgcgtg catcgaaatc gcgccgaagg agcttatcgt
     2641 cgtcgaaacg ggcgcacgca cgccgttgac caacgtctcc gcgtcgcgca tcgagaacct
     2701 cgcgatcgac ggcaaagcga tcgacggcga tctgaaattc gggaatgcga atgcggacag
     2761 ccgaggagtc gcgagactgc cggtgacact cagtggacat tttccgaacg gtgtggcgaa
     2821 gacgacggtg atgatccgag cgaacgagct cgcagcgccg gtgtttgtca cgtacgagat
     2881 tcacgcgcgt ttccatccgt ggcttctctt cctcgcaata ctcatcggtc tccttctcgg
     2941 atacgcgatg cgcacgttgc tcgtgcggtt cgtcgagagc aggactgcct acatcgcggc
     3001 gctcgatctg cgcgacaaga tcgattcaga gaaaaagaag tattccgatc ccacattcat
     3061 cggcatcgtc gacccactcc tcacgcgcct gcgcgatgcg atcgacaaac ggcctgttga
     3121 tctcacggcc atcgtgaacg acatcaagga gaaatacaac gccgcggtca gtacgctgca
     3181 ggacaaaatc aaggccgttc gcgcggcatt cgacgcctcg gtcagccttc tgtcaccgga
     3241 cgactttcct cccgacatcg cgaaggcgct cacggaagca cagcagacga ttgatgaagc
     3301 acgcaagctc gtcgacagcg atccgaccgg cgcgcagaac gagctcacgc aagcgacggc
     3361 gaacctgcag gccgatgtcg acagcgccgc ggccggatgg cgcgtcgaaa tgcaagtagc
     3421 gcttgccgat gccgtgcgga gtccgctccc gccaaatttc gatgagccgt tcaggtcggc
     3481 gctcgcaacg ctgaaaacag ggctcgagca attgccgatc ggacaatcga aagacacggt
     3541 gacgatcctc agcgcggtcc acggcctgca gttcacgctg cgcttcaatc tggtgaagcg
     3601 cgtgctcgat ccaatgcaga cctacgggca gtcggtcatc gacgtgttga agcagaaaag
     3661 tgagccggtg gccggatccg aagctgcgct gcaggcgttt gcggcagcca atggaccggc
     3721 gccgctggca cagctcgatg cgttgctctc agcgctcgcc gctgacatca aggccgcggg
     3781 cgacaagaac ggcgtcaacg ttcagaacga tctggcggca gggaagtatc tcgaggccgc
     3841 gcgagccgcg gtgaagcaac caccgatcgc cgcaatcggt agcttaccgg gcacgctcgc
     3901 cacacctctc gcagcggcgg gggaggcgtt ccccgtttcg gggcttcagt tcgtgcagcg
     3961 ggcgtttccg cacgcgcagt tggcattgcc ggatacggac acgatcgagc ggcagcgatc
     4021 ggtctgggag atcgcggccg cgaaactcgc gcgcttcgcc atctccgcgc tcgggatttc
     4081 gatggccggt ctgctcgtgc tgctgccggc gttcgacggc acctggcgcg gtctgctcac
     4141 agggctcttc tggggttacg caggcgacat tagcgtcgac gcactgaccg aagcggcgaa
     4201 gaaatatcga acgtaacgct actcgagtgg cgtgatcccc tgcgcgcgca ggtcgctgag
     4261 cgcgaggcgg cggaagtagt caggattccc cttcgcgctc gcctgccagt cggccgcttt
     4321 cttctcgcct tcggcgcatg catcttcgaa cgtcgccgtc ccgatcatcg tcgtttcgac
     4381 gtacttccat tgatcgctgc cgccgttgcc gtcgcgccac gtttcccagg ctaggaatgc
     4441 gtgtccgggg atcagcacga tggcggggtt gagcgaaatc gcctccagca ggctcgccat
     4501 cagcagcgtg ctgtcgatgc agttcgcgtt cttgtccttg agtgtttcgg cggggcggcg
     4561 cacgcgctgg ttcgctgcgc cgctctcacg cgtgaactgg atcaccgact gcacgtagcg
     4621 gattccgagc gccttgagcg catcgaatgt cgcttggacc tgcggctgca ctctggtgtc
     4681 cgcgatctga tagccgacca gttccttttt cggatgcgcg tcggtcacgt tgcgcacaaa
     4741 ctccatcacc gcgggagcgt tcggagtcac gaacgcggca aagtaggcag tcagatcgcg
     4801 ccactgtccg gtcgacgggt cgagcaccga cagcggtgcg gtcgtgcgcg gcacgagcca
     4861 taccgggatc gtcttgtgca gctccgattt cgcatcgaga tcgcgcactt cgacattgac
     4921 cgtcgctcgt gtcagctcgt tgaccagctc gatctggcgc gggaaaaacg tcggcatctg
     4981 tttgatggac ttcgtctctc cgttgatgat ctccatcgtc tcgacggcgc gtgccgaa
//
LOCUS       DATLVW010000590         6627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_235175, whole genome shotgun sequence.
ACCESSION   DATLVW010000590 DATLVW010000000
VERSION     DATLVW010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235175"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VNN25_17350"
     CDS             complement(<1..771)
                     /locus_tag="VNN25_17350"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXH93353.1"
                     /translation="MKRALIFGVSGQDGAYLSRLLLDRGYEVHGVSRDAESQPFHRLS
                     TLGIRDRVITHSAAARDFRELLQLIDSIRPDEIYNLAGQSSVALSFSQPVETMDSIAQ
                     ATLVMLEVIRYLKLPVRLYNSASSECFGDTPPGTASDESTPFFPRSPYAMAKAAAHWT
                     TVNYREAYGIYACSGILFNHESPLRSERFVTRKIVTAASDIAAGNRRRVTLGRLDVSR
                     DWGYAAEYVEAMWRMLQQDKPQDFVIATGESHTLEELTA"
     gene            complement(1007..2059)
                     /locus_tag="VNN25_17355"
     CDS             complement(1007..2059)
                     /locus_tag="VNN25_17355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH93354.1"
                     /translation="MRVLHIGTDSFGGYGGIALYNRDLIAAIASHPVVTEVVVVPRLL
                     PSVPEPLPAKVAFLDQAAAGRLAYLNTLRKLRGPFDLVICAHVNLLPVARMFSKHPML
                     MIYGIEAWKPLREMLSNRLARRCSAVVSISDITLDRFLGWSGYEGNSHLLPNAIRAAW
                     YGIRPRNEGLAARYRLEGKRVLMTLGRVVAAERYKGFDEVLEIMPALDAGISYVVAGG
                     GNDVPRLKRKAARLGVEKRVIFTGLVPEHEKADLYNLADVYVMPSRGEGFGFVLLEAM
                     ACGVPVIASRLDGGREAVRQGELGQLVDPTSPAEIRVAITDALASGGKSVPQGLEYFS
                     FENFESRTHAIIESLV"
     gene            complement(2056..3816)
                     /gene="asnB"
                     /locus_tag="VNN25_17360"
     CDS             complement(2056..3816)
                     /gene="asnB"
                     /locus_tag="VNN25_17360"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH93355.1"
                     /translation="MCGIAALFAYGSDAPPVDAVEIDAITERMRPRGPDAGGTWISPD
                     QRVALGARRLAIIDLTDEGTQPMTDTDGDLRIVFNGEIYNHHELRARLKRLGATFHST
                     SDTEVLLQLYRRDGPGMVDLLRGMFSFAIWDPGPRRMFVARDPYGIKPLYIADDGKTF
                     RAASTVKALLAGGQISRARNPAGVAGFWLMGSVPEPFTIFEEIRAVAAGTSFFVDERG
                     IHDERHYYSIATTLGQGVRDEPAARLVDPHVLVRELVTDSLRHHLISDVPVGAFLSAG
                     IDSCALVALATEAFFQPPRTVTLRFEEFQGHDVDEAPLAERFAQRIGSEHVTRLVARD
                     EFIADLPRLFDAMDQPTIDGTNTWFVSKAAAERGLKVAISGLGGDELFGSYPSFRTLP
                     RIVMGARVPFAASALSRLASRPKAKALARYGHSWAGAYLLQRGLFLPDDLPALIGAEQ
                     AEEGLKRLSLIEHIASVLDPDPSSAFGRVATLEASLYMRNQLLRDTDWASMAHSLEVR
                     TPLVDATLLRQIAPLLLVEQQRCKHHFAKSPRTPLPDWLSERRKTGFIVPLADWMNLP
                     ADGTSTRMRSWARVVMERWS"
     gene            complement(3821..4915)
                     /locus_tag="VNN25_17365"
     CDS             complement(3821..4915)
                     /locus_tag="VNN25_17365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXH93356.1"
                     /translation="MAQYAVPLLLLFIASALTSVITTGAVKRIAEEVGAIDRPNERKF
                     HAAETPRMGGLAIIFGFGFPLMLLAANTHAAELVSKNLTYLFAVLASGSLIVGLGVYD
                     DLLGSDAPKKFIVQTAAAIILVSFGFRFSSFSIAGFTIRLGILGSVVSVIWIVGVINA
                     VNFIDGIDSLATLVSITIAGAFGVIAIIRHDVFSLVIMTSLTGSLIGFYRWNRPPAKI
                     FMGDTGSLFIGLLLAACSIARPIKSPTALIVGGPMLALALPVLDTLIVMKQRFGAESA
                     IGARVTRMFNADRRHFHHILVEKYGSITKAILSIWFITLLFAVAAVLTVIEELRNTGY
                     YVGVAGFIAMLFLRYWFRRHLGQAPVERQA"
     gene            5131..>6627
                     /locus_tag="VNN25_17370"
     CDS             5131..>6627
                     /locus_tag="VNN25_17370"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HXH93357.1"
                     /translation="MAELPGVERETLEVDVVIVGAGPAGLAAAYRLAELVKAHNESNA
                     EKKLEGLSIAVLEKGQEIGSHGISGAVMDPRGIAELMPDWLERGCPIESPVTDDGFWY
                     LFESMKIAAPIMPPPLQNHGNYVISLGEMVRWMAPIVGEMGVDLFAEFAASRVLIEDG
                     RVVGVRTGDKGIDKNGQPKANFEPGVDIRAKVTILAEGPRGTLTKQLATSFDLYAGKN
                     PQVYSVGVKELWQLPDDRFPLGNVIHTLGWPLDGDTFGGGFIYGMKDRIIDIGLVVGL
                     DYKNPTLDPHQEFQRYKTHPSIRPLLEGGKMISAGAKAIPEGGYFSMPRMYGPGFLIT
                     GDSAGYLNGARLKGIHLGIKSGSLAAETTFQALLNNDYSAAQMSEYEAMFEVSWAREE
                     LWKQRNFHQAFEYGQLAGLVNAGLGIVTGGRGYGVMNRLEGHAGHERMERIQRYFGAD
                     HPHPPEKIKFDGVLTFDKVTDVYNGGVIHEENQPAHLLIADTEVCITRC"
BASE COUNT         1316 a         1926 c         2217 g         1168 t
ORIGIN      
        1 ggcggtgagc tcttccagcg tgtggctctc gccggtggcg atgacgaaat cctggggctt
       61 gtcctgctgc agcatgcgcc acatggcttc gacgtactcg gcggcgtaac cccaatcgcg
      121 ggagacgtcg aggcggccga gggtgacgcg gcggcggttt ccggccgcga tatccgaggc
      181 ggcggtgacg atttttctgg tcacgaatcg ttcggagcgc aagggcgact catgattgaa
      241 gaggatgccg gagcaggcgt agatgccgta cgcctcgcga taattgacgg tcgtccagtg
      301 cgcggcggct ttggccatcg cgtaggggct gcgcggaaag aatggcgtcg attcgtcgct
      361 ggcagttccg ggcggtgtat cgccaaagca ttcgctcgat gcggagttgt aaaggcgcac
      421 gggcagcttc aggtagcgga tgacttcgag catgacgagc gtggcctgcg cgatgctgtc
      481 catcgtttca accggctgcg aaaacgagag ggcgacggag ctctggccgg cgaggttgta
      541 gatctcgtcg ggacggatgc tgtcgatcaa ctggagaagc tcccggaaat cgcgcgccgc
      601 ggcggagtga gtgatgacgc ggtcgcggat gcctagagtg ctaagacggt ggaacggctg
      661 cgactccgcg tcgcgcgaaa ctccatgaac ttcgtatcca cgatcgagca gcaagcggga
      721 gagataggcg ccatcctgcc ccgacacccc gaagatcaac gcgcgcttca cgggagcatt
      781 atgccgaaga gacagagatc agagacaaga gatcggagat caggggacag tggcccggct
      841 cagcggccac gggagcctct ctgttctcgc cagacatcag agagaacagg ccgggtagag
      901 gtcgcaatca gtaggtgcga atcgcgatct ctcatctatg cccctgatct cttgtctctg
      961 atctctgtct ctgattgtct ctgatccctt gtctctaaga atgggatcaa accagcgatt
     1021 cgatgatcgc gtgtgttcgt gactcgaagt tctcgaaact gaagtattcg agcccctgcg
     1081 gaacgctctt gcctccgctt gcgagggcgt cggtgatggc gacgcgaatc tcagcaggac
     1141 tggttggatc gacgagctgc cccagctctc cctgacgaac ggcttcccgt ccgccatcga
     1201 ggcggctggc gatgaccggg acgccgcacg ccatcgcctc gagcaggacg aacccgaagc
     1261 cttcgccccg actgggcatg acgtagacgt cggccagatt gtagaggtcc gccttttcgt
     1321 gttcgggcac gagcccggtg aagatgactc gcttttccac gccgagcctc gctgccttgc
     1381 gcttcaagcg ggggacgtcg ttgccaccgc cagcgacgac ataggagatg ccggcgtcaa
     1441 gggcgggcat aatttcgagg acctcgtcga agcccttgta gcgttcggcc gcgacgacgc
     1501 ggcccagagt catgagcact cgttttccct cgaggcggta gcgagcagcc aatccctcat
     1561 tgcgaggccg aatcccgtac caggctgcac ggatggcatt gggaagcagg tgcgagttcc
     1621 cttcgtagcc ggaccagccc agaaagcgat cgagcgtgat gtcgctgatg gagaccacgg
     1681 cgctgcatcg gcgtgccaga cgattggaga gcatctcgcg cagcggcttc catgcctcga
     1741 taccgtagat catgagcatc ggatgcttgc tgaacatcct cgcgaccggc agcaggttga
     1801 cgtgggcgca gatgaccagg tcgaacggtc cgcgcagctt gcgaagcgtg ttgaggtacg
     1861 ccagtcgtcc ggcggcggct tgatcgagga atgccacttt cgcggggagc ggctctggta
     1921 cggaggggag aaggcgggga accacgacca cctcggtcac gacaggatgc gaggcgatcg
     1981 ccgcgatgag atcgcggttg tagagggcga tgcctccgta ccctccgaac gaatccgtgc
     2041 cgatgtgcag caccctcacg accagcgctc catgaccacc cgtgcccagc tacgcatgcg
     2101 cgttgacgtt ccatcggcag ggagattcat ccagtcggcg agtgggacta tgaatccggt
     2161 cttcctgcgc tcgctgagcc agtccgggag gggagtgcgg ggactcttgg cgaagtgatg
     2221 tttacagcgc tgctgctcca cgagcagaag cggggcgatc tggcggagaa gggtggcatc
     2281 gacgagaggc gtgcgcacct ccagcgagtg ggccatgctt gcccagtcgg tgtcgcgcag
     2341 cagttgattg cgcatgtaga gcgatgcttc cagcgtggcc acgcggccga aggcggagct
     2401 tggatccgga tcgaggacgg aggcgatgtg ctcgatcaat gaaagacgct tcaggccttc
     2461 ctccgcttgc tccgcgccta tgagtgccgg aaggtcatcc gggagaaaga ggcctcgctg
     2521 caggagatag gccccggccc acgaatgtcc gtagcgggcc aacgctttcg ctttcgggcg
     2581 gcttgcaaga cggctgagcg ccgaggcagc aaatgggacc cgggcgccca tgacgatgcg
     2641 cggcagagtg cggaatgagg ggtaactgcc aaagagctcg tcgccgccaa gtccggagat
     2701 ggccactttc agaccgcgtt ccgcggctgc tttgctgacg aaccaggtgt tggtaccgtc
     2761 gattgtgggt tgatccatcg cgtcgaagag gcggggaagg tcggcgatga attcgtcccg
     2821 cgccaccagg cgggtgacat gctcggaacc gatccgttgc gcgaaacgct cggcgagggg
     2881 ggcctcgtcg acgtcatgac cctgaaactc ctcgaaacgg agggtgaccg ttcgcggcgg
     2941 ttgaaagaac gcctctgtcg ccagagcgac gagggcgcag gagtcgatcc ctgcggagag
     3001 gaacgcgcca accgggacgt cggagatgag atggtggcgg agcgagtcgg tgacgagctc
     3061 tcgcacgaga acgtgagggt cgacaaggcg cgcagcgggc tcgtcgcgaa caccctggcc
     3121 gagtgtcgtt gcgatcgagt agtagtggcg ttcgtcatga atccctcgct catcgacgaa
     3181 gaacgaggtt cccgcggcca ctgccctgat ctcttcgaag atcgtgaacg gctccggcac
     3241 gctgcccatg agccagaagc cggcaacacc ggctggattc ctggcgcgcg agatctgtcc
     3301 gcccgcaagc agtgccttga cggtcgatgc cgcgcggaat gtcttgccgt cgtcggcgat
     3361 gtacagcggt ttgattccgt agggatcgcg cgccacgaac atgcgccgcg gtcccggatc
     3421 ccagatcgcg aacgagaaca tgccgcgcaa caggtcgacc atccctggtc catcgcgccg
     3481 gtagagctgc agcagaacct cggtgtccga tgtggagtgg aacgttgccc ccagccgctt
     3541 cagtcgtgcg cgcaactcgt gatggttgta gatctcgccg ttgaagacga tgcgcagatc
     3601 gccgtcggta tcggtcatcg gctgcgtgcc ttcgtccgtc aggtcgatga tggcaagacg
     3661 ccgcgcgccg agcgctactc gctggtcggg cgagatccag gtgccgccag cgtcggggcc
     3721 gcgcggacgc atgcgctcgg tgatggcgtc gatctcgact gcatcgaccg gaggtgcgtc
     3781 gcttccgtag gcgaaaagag cggcgattcc gcacatcgcg tcaggcctgc cgctcgaccg
     3841 gcgcctgccc caggtggcgc cggaaccaat agcggagaaa aagcatggca atgaagccgg
     3901 cgacgccaac gtagtagccg gtgttcctca attcctcgat cacggtgagc accgcggcga
     3961 cggcaaagag cagcgtgatg aaccagatgg agaggatggc ttttgtgatg ctgccgtatt
     4021 tctccacgag gatgtgatgg aagtggcggc ggtcggcgtt gaacatccga gtcacgcgcg
     4081 cgccgatggc cgactccgca ccaaaccgct gcttcatgac gatgagcgtg tcgagcactg
     4141 ggagcgccag ggcgagcatc ggtccgccga cgatcagggc agtgggcgat ttgatcggac
     4201 gggcgatcga acaggccgca aggagcaggc cgatgaaaag gctgccggtg tcgcccatga
     4261 agattttggc gggcggccgg ttccaccggt aaaacccgat caggcttccc gtcagcgagg
     4321 tcatgatgac gagagaaaac acgtcatgac ggatgatcgc gatgaccccg aaagccccgg
     4381 cgatcgtaat ggacaccagc gtcgcgagcg agtcgatccc gtcgatgaaa ttgacggcgt
     4441 tgatgacgcc cacgatccag atcacggaga cgaccgatcc gagaatgccg agccggatgg
     4501 tgaaccccgc aatcgagaag gatgagaacc ggaacccaaa actgaccagg atgatggcgg
     4561 ccgcggtctg aacgatgaat ttcttcggcg cgtccgagcc gagaagatcg tcgtaaaccc
     4621 caagtccgac gatgagcgag ccgctggcca ggacggcgaa gaggtacgtg aggtttttcg
     4681 agaccagctc ggcggcgtgg gtgttggccg cgagcagcat caacggaaac ccgaagccga
     4741 agatgatggc cagcccgccc atccgcggcg tttctgccgc gtgaaacttc cgctcgttcg
     4801 gacgatcgat cgctcccact tcctcggcaa tccgcttgac cgcgccggtc gtgatcacgg
     4861 atgtgagcgc gctggcgatg aaaaggagga ggaggggaac ggcgtattga gccatcggaa
     4921 tgggtgagaa gaatagcagc ggggggtggg gagtggggag tcgagagtgg atttaggcag
     4981 atcgaatgat ggcggttgtg cgtatcggtg gccgggaatc gtgcgtcgga gtgatacaag
     5041 cgagcgtcga tcagctttac acccacaccc tacccccaac accccacccc ccacgtatag
     5101 aatgccgcga cttttctgaa ggagcaacgc atggctgaac ttcccggcgt cgagcgggag
     5161 accctcgagg tcgatgtcgt gatcgtcggt gccggaccgg ccgggctggc tgccgcctac
     5221 cggctcgccg agctggtcaa ggcccacaat gaatccaacg ctgagaaaaa gctggagggg
     5281 ctttcgatcg ccgtcctcga gaaagggcag gagatcgggt ctcatggcat ttccggagcg
     5341 gtcatggatc cgcgcggaat cgcagagctg atgccggact ggctcgagcg gggatgcccg
     5401 atcgaatcgc cggtcaccga cgatggcttc tggtacctct tcgagtcgat gaagatcgcg
     5461 gcgccgatca tgccgccgcc tctgcagaac cacggcaact acgtcatttc cctcggcgag
     5521 atggttcgat ggatggcgcc gatcgtcggc gagatgggcg tcgacctctt cgcggagttc
     5581 gctgcctctc gcgtcctgat cgaggacgga cgcgtcgtcg gcgtccgaac aggcgacaaa
     5641 ggcatcgaca agaacggcca gccgaaagcg aacttcgagc ctggtgtcga catccgtgcc
     5701 aaggtgacga tcctcgcgga agggccgcgc ggcacgctca cgaaacagct tgcaaccagc
     5761 ttcgatctgt acgccggaaa gaatccgcag gtctactcgg tcggggtgaa ggagttgtgg
     5821 caactgcccg acgaccgatt cccgctcggg aacgtgattc acactcttgg ctggccgctc
     5881 gacggcgata ccttcggcgg tggcttcatc tacgggatga aagaccgcat catcgacatc
     5941 ggtctcgtcg tcggactcga ttacaagaat cccacgctcg atccgcatca ggagtttcag
     6001 cgctacaaga cgcatccttc catccggccg ttgctggaag gcggcaagat gatcagcgcc
     6061 ggcgcgaaag cgattcccga gggcggctac ttttcgatgc cgcggatgta tggtccgggc
     6121 tttctcatca cgggcgactc ggccggctac ctcaatggcg cgcgtctcaa aggcattcac
     6181 ctcggcatca agtcgggatc gctggctgcc gagacgacgt tccaggcatt gctcaacaac
     6241 gattattcgg ccgcgcagat gagcgagtac gaggcgatgt tcgaagtctc atgggcgcgc
     6301 gaggagctgt ggaagcagcg caatttccat caggcgttcg aatacggaca gctcgcaggg
     6361 ttggtcaacg ccggtctcgg catcgtgacc ggtggccgcg gctacggcgt catgaaccgt
     6421 ctcgaaggac atgccggtca cgagcggatg gagcggatcc agcgctactt cggcgccgat
     6481 catccgcatc cgcccgagaa gatcaagttc gacggtgtgc tgacattcga taaggtcacc
     6541 gacgtctaca acggcggcgt cattcacgaa gagaatcagc cggcgcatct gctgatcgcg
     6601 gacaccgaag tctgcatcac gcggtgt
//
LOCUS       DATLVW010000591         7559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_194490, whole genome shotgun sequence.
ACCESSION   DATLVW010000591 DATLVW010000000
VERSION     DATLVW010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194490"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VNN25_17375"
     CDS             complement(<1..1506)
                     /locus_tag="VNN25_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HXH93358.1"
                     /translation="MKRAFIAATVPLLIASIATARDLPNFDAAATQRTAPSAVAQASS
                     SKIPTEWYAQFGTPSFRWIGGAPGGAVTASAAVSGFDGAYVSEVHDTGRGAIVTRYRQ
                     RIDGIEVFRNELNVVTARDGATVAISGHLAGPAARAAAKSGELFRLSEAATVAIASGD
                     LDANGPAASRLSRVWFDPGTSLEPVYSIELATDTEMFLYVISAADGRLLFRKNLTEDA
                     GTPFTYRVWAETTGIKRPLNGPQGLVGDPNPTGTNDGFQAPFVETGLVTLANGPISTG
                     DPWLAASATQTTGNNVDAYADLSAPDGFSTGDERASTTSLRTFDYRPDMQSQPGVNAT
                     QRKAAVTQLFYDVNFLHDWFYDSGFNEAARNAQTDNYGRGGVAGDSIRAEAQDYSGRN
                     NANMSVPADGGRPRMQMYLFDAVTFRTLSVDSPATLTGQYAVGTAAFGAQAFNLSGNV
                     VAASPADGCAAIGSSVAGKIAFVNRGTCSFTVKAQNAQNAGALAVIVGNVAD"
     gene            1718..2212
                     /locus_tag="VNN25_17380"
     CDS             1718..2212
                     /locus_tag="VNN25_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXH93359.1"
                     /translation="MRDALRVRLGSFLIVLLIAQSIGAGDRPEAVVKYRQSVMKAMAA
                     HMTAISMVARQEIGNDRQLAAHAEAIHGLSVGLGEFFPAGTDAAKVKSAARPEIWRQP
                     AEFKAAGAKLESESAKLLQLAQQRGTASNAAPNLHERLVSQVDAVGAACKACHKTFRK
                     SDTE"
     gene            2220..4193
                     /locus_tag="VNN25_17385"
     CDS             2220..4193
                     /locus_tag="VNN25_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HXH93360.1"
                     /translation="MRLRTHLAVGLIAIAALVSCRKEQTPASRTAPAPSKKATVTASD
                     DWEELPSRNAYAGSASCRECHEKNFLRWQHDWHSRALAPASATSVVGSFANAHFKGTS
                     SEAWMTRRGDSFVMRTRNAQGELGDYPVAWVIGGKRMQDALTIFPDGRWQVLPVYFHV
                     TGDGAWVDYNEHKQGVVSPTHPFFWTNFRRTANKECLECHSTGLDLRYDAAAQSWSTT
                     FADAGVACEACHGPGARHAETKSKADIIHPGHIDAALALSICARCHGPHEPLFPLLDQ
                     KDQFRPGQRYDDKYQALVIVDSTARSGEYFADGRPNSSSFEYQALVQSRCYRVGGATC
                     LTCHTAPHKEHGPNEMKSDADRSCRDCHSAIAKEGRAHTHHPAKAASCTGCHMPRLVS
                     GVLDKFPDHSIDIPNARNTLRHGVPNACGVCHAKKPPELIEQTIASWWPQAAERQQRR
                     SVLADAIDEASATQSLPSLTAVVRDLHEAPTLRGATAVILGQRFPTAAASILEPLLAD
                     RDPLVRARLIEALGYARAGSAADAIARYGTDSSLHVRQAAGLVLAAFGDARGFKMLQA
                     LAADPASRALVKPHIMLGIAAANRGDFITARSELELALVEAPYVPDALVFLADIHARQ
                     SDLPGARSLLEEALRYDPQHAGAIKRLRALGAR"
     gene            4356..4580
                     /locus_tag="VNN25_17390"
     CDS             4356..4580
                     /locus_tag="VNN25_17390"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXH93361.1"
                     /translation="MTINYREHDPPHFHAWYAGSDISVRISDGSVSGQLQRRALALVL
                     EWWHLHRTELLEDWHLAQAGQSLKKIDPLD"
     gene            4586..4828
                     /locus_tag="VNN25_17395"
     CDS             4586..4828
                     /locus_tag="VNN25_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXH93362.1"
                     /translation="MYYTVIDARYVHDFTIWAKFADGIEGEIDLSDALWGPVFEPLKD
                     VNYFRNFSLAEYGTICWPNGADIAPEFLYGKVRVHA"
     gene            4889..6247
                     /locus_tag="VNN25_17400"
     CDS             4889..6247
                     /locus_tag="VNN25_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93363.1"
                     /translation="MRKSLGIAFVVALVCLGAFSQTTIRNDAVTQVSPEGDIVGFFGQ
                     PVTFATHAELVSYGFSFGPSDGSIGAIPAAGGTYTFYGSAGSTAGCSASPNVHGTFPF
                     NGTLDRVSGVTGGCRRMFGPGDAPAGYVFDKDYAGGGGVVRFASGGKSGWLMPFHAEY
                     HWQNQANPPSFLCSAGGNSASMVPCFYSSIGLAVSTDDGKTFRVAGQIVQPSEPLSLF
                     EGGGTNMAVGYGSLLVADANGKHLDNPPPDPANAYFYVFYNDRLQGAPGACSNAICLG
                     VARAVYADVVTAVLSGDGHQVSQLFHKYDGASPNPWTQPATAFVQGNATPDLSGTAGK
                     YLPLWTDENGASAIVLYDRSFDVYLAVYVSGLGVSIKARASSDMIHWSEPISAPFTEA
                     GRALFYPTLIGETGDPTIGGLAPRVYFTSFPTDQFPNYSTSVFESVTLTLARQRRRAT
                     KH"
     gene            6291..6902
                     /locus_tag="VNN25_17405"
     CDS             6291..6902
                     /locus_tag="VNN25_17405"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HXH93364.1"
                     /translation="MKARHLFALSLALALLIANSAFAAQSLDCFYAARPTAKNPDPEM
                     SLHRNCAVVSGDGSVRIVARHLQALDFNADGLATFNAERRGWFYVLRNGRILEVLSED
                     NGADDFVEGLVRGRRHGKVAFFDRSFRMVLPPIYDFAWPFENGLAMVCSGCTQRTDAS
                     DPDHHGLVDGGLWGYIDHHGREVIPVKYSRDEALRQEGLLSKR"
     gene            6937..>7559
                     /locus_tag="VNN25_17410"
     CDS             6937..>7559
                     /locus_tag="VNN25_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXH93365.1"
                     /translation="MSNRIRALLLLIATLATTSTFAADIRWQPQRTWVFVVGTLEWND
                     SETFASFPKANRRDAQLVRFFRQKGVTANHIVYLADKQATTSHAQAALDRLLAQTAPG
                     DLLFFYFTGHGYQSDDGKKTFSATYDASDHRAGWNVDAIVRTIAARFRGDRVILAADC
                     CYSGALVDRVKRLRGRQSFACFASSTSKDSSTANWTFTEMLLAGLQGR"
BASE COUNT         1391 a         2341 c         2386 g         1441 t
ORIGIN      
        1 gtcggcgacg ttgccgacga tcaccgccaa cgcgcccgcg ttttgtgcgt tttgtgcttt
       61 gacggtgaag ctgcaggtgc cgcgattgac gaaggcgatc ttgccggcga ccgaggagcc
      121 gatcgccgcg cagccatccg ccggcgaggc cgcgaccacg ttcccgctca gattgaaggc
      181 ctgcgcgccg aaggctgcgg taccaaccgc atattggccg gtcagtgtag ccggtgaatc
      241 gacactgagc gtgcggaacg tgaccgcgtc gaagaggtac atctgcatgc gcggacggcc
      301 gccatcagcc gggaccgaca tgttcgcgtt gttgcgtccg gagtagtcct gcgcctctgc
      361 gcggatcgag tcgccggcta caccgccacg tccgtagttg tcggtctgcg cgttgcgtgc
      421 agcctcgttg aacccggagt catagaacca gtcgtgcaga aaattgacgt catagaagag
      481 ttgcgtgacc gcggccttgc gctgcgtcgc gttcactccg ggctgtgatt gcatgtcggg
      541 ccggtagtcg aatgtgcgca gactcgtcgt ggaggcccgc tcgtcaccgg tcgagaatcc
      601 gtcgggcgcg ctgaggtcgg cgtaggcgtc tacattgttg ccagtggttt gcgtcgcact
      661 cgccgccagc cacggatcgc ccgtgctgat cgggccgttt gcgagggtca cgagtcccgt
      721 ctcgacaaac ggagcctgaa atccatcgtt ggtgcccgtg ggatttggat caccaacgag
      781 tccctgcgga ccattgagcg ggcgcttgat ccccgtcgtc tccgcccaaa cgcgatacgt
      841 gaacggcgtg ccggcgtcct cagtgaggtt cttccggaac aggagccgcc catcggccgc
      901 ggagattacg tagaggaaca tctccgtgtc tgtcgcgagc tcgatcgaat acaccggctc
      961 gagcgatgtg ccggggtcga accagacgcg gctcaggcgc gatgcagcgg ggccgttggc
     1021 gtcgagatcg cccgacgcaa tcgcaaccgt cgcggcttcg gatagacgaa acagctcccc
     1081 actcttcgcc gccgcacgag ccgcgggacc cgcgagatgc cccgagatcg ccaccgtcgc
     1141 accatcgcgc gccgtgacga cattcaactc gttgcgaaac acctcgatgc cgtcgatgcg
     1201 ctgacggtat cgcgtgacga tcgcgccgcg gcctgtgtca tggacctccg aaacatacgc
     1261 gccatcgaaa ccgctaacgg ccgcggacgc agtgacggcg ccgcctggcg ctccaccgat
     1321 ccaccgaaag gacggagtgc cgaactgcgc gtaccactcc gttggtatct ttgagctcga
     1381 ggcttgggcg acggccgagg gtgcagtgcg ctgcgtcgcg gcagcatcga agttaggaag
     1441 gtcgcgcgcc gtcgcgatgg aggcgataag tagcggtacg gtagccgcga tgaacgcccg
     1501 cttcatggag gcaacgatgc taacaggtct caatttgctt gcgggagaga cgactcacgc
     1561 ggagacgcgc agaacgcgaa gagaagagct ccgtgatctc cgcgtctccg cgtgaaacaa
     1621 gaggtgaaca cacgcggatc agcaagttaa gaccggctgc acacccgttt tgcgtttcct
     1681 gactccccac ctcagtttcc actatccttc gccaacgatg cgggacgcct tgcgggtgcg
     1741 gcttggttcc tttttgatcg ttcttctgat tgcccagtcg atcggcgcgg gggaccggcc
     1801 ggaggcggtg gtgaagtacc ggcagagtgt gatgaaggcg atggcggcgc acatgaccgc
     1861 gatctcgatg gtggccaggc aggagatcgg gaacgatcgc cagcttgccg cgcacgccga
     1921 ggcgattcat gggttgagtg tgggattggg ggagttcttc cccgctggga ccgacgcggc
     1981 aaaggtgaag agcgcggcca ggccggagat ctggcgtcag ccggcggagt tcaaggcggc
     2041 cggagcgaag ctggagagcg agagcgcaaa gctgttgcag ttagcgcagc agaggggtac
     2101 cgcgtcgaat gccgctccga acctccacga gcgcctggta tcgcaggtcg atgcggttgg
     2161 cgcggcgtgt aaggcgtgcc acaaaacgtt ccgcaagagc gacaccgagt agcgcgcgga
     2221 tgcgattgcg tacgcatctc gcggttggac tgatcgcaat cgcggcactc gtatcgtgtc
     2281 gaaaagagca gaccccggca tcgcgaaccg cgccggctcc ttcgaagaaa gcgacagtca
     2341 ccgcgagcga tgactgggaa gagctgccat cccgtaacgc ctatgccggc tctgccagtt
     2401 gccgggagtg tcacgagaag aatttccttc gctggcagca cgattggcac tcgcgcgccc
     2461 tcgcgccggc ctcggcgacg agtgtcgtcg gatcgtttgc caacgcgcac ttcaagggta
     2521 catccagcga agcatggatg acgcggcgcg gcgactcgtt cgtcatgcgt acgcggaatg
     2581 cgcaaggaga gcttggcgac tatcccgtcg cctgggtcat cggcggaaaa cggatgcagg
     2641 atgcgctcac catctttccc gacggccggt ggcaggtcct tcccgtctac tttcacgtta
     2701 ccggcgacgg cgcctgggtc gactacaacg agcacaaaca gggcgtggtc tccccgacgc
     2761 atcccttctt ctggaccaac ttccgccgca cggcgaacaa ggagtgtctg gagtgccact
     2821 ccaccggcct cgatctgcgt tacgacgcgg cggcacaatc atggtcgacg acgttcgcgg
     2881 atgccggcgt ggcgtgtgaa gcctgtcacg gccccggagc gcggcacgcc gagaccaaga
     2941 gcaaggccga catcatccat ccgggacaca tcgacgccgc gctggcgttg tcgatctgcg
     3001 cgcgctgcca tggtccgcac gagccgctct ttccgctgct cgatcagaag gatcagttcc
     3061 gcccaggtca gcggtacgac gacaaatatc aggcactcgt gatcgtcgac agcaccgcac
     3121 gatcgggcga gtatttcgcc gacggacgcc cgaacagctc gagcttcgag tatcaggccc
     3181 tcgtccaatc gcgctgctac cgcgtgggag gagcgacctg tctcacctgc cacaccgccc
     3241 cgcacaaaga gcacggtccc aatgaaatga aaagcgacgc cgatcgttcg tgccgcgatt
     3301 gccacagcgc cattgcgaag gaagggcgcg cgcatacgca ccatccagcg aaggcagcga
     3361 gttgcaccgg ctgtcacatg ccgcggctcg tcagcggggt gctcgacaag ttccccgacc
     3421 acagtatcga catccccaac gccaggaaca cgctccggca tggagtcccg aatgcctgcg
     3481 gcgtttgtca cgcgaagaaa ccgccggagc tgatcgagca gacgatcgcc tcctggtggc
     3541 cgcaagccgc ggaacggcag cagcggcgaa gcgtgcttgc cgatgccatc gacgaagcgt
     3601 ctgcgacaca aagccttcct tcgctcaccg ccgtcgttcg tgaccttcac gaagctccca
     3661 cgctccgagg cgcaaccgcc gtcatcctag ggcagcgctt ccccacggca gccgcgtcga
     3721 tcctcgagcc gctgctggcc gatcgcgatc cgctcgtacg agcgcggctg atcgaggcgc
     3781 tcggctatgc gcgggccggc agtgccgccg atgcgatcgc gcggtatgga accgattcgt
     3841 cgctccacgt ccgtcaagcc gcgggtctgg ttctggcggc gttcggtgat gcgcgcggct
     3901 tcaagatgct tcaggcgctc gccgcggatc ccgcatcgcg cgccctggtg aagccgcaca
     3961 tcatgctcgg gattgccgcg gccaatcgcg gggacttcat taccgcccgc agcgagctcg
     4021 agctcgcgct cgtagaggct ccctacgtac cggacgctct cgtcttcctg gccgacattc
     4081 acgcgcgcca gagcgatttg cccggcgcgc gatcgctcct cgaagaagcg cttcgctatg
     4141 acccccaaca tgccggtgca atcaagcgtc taagagcgct gggtgctcga tagggtgtgt
     4201 tagctacgag ttatgcacgt cgtaaggagt gcggtcgtct cgaccgcggg cactcgggcg
     4261 tcctcgcccg agtgcggtca gggtggaggt cgaaacgttt cgttcgtgtg tagaatcgcc
     4321 tcgcgcccga gatcagccgc ttcttcggaa tcatcatcac gatcaattac cgcgagcacg
     4381 atccgccgca ctttcacgca tggtacgccg gctccgacat ctcggtccgg atctccgacg
     4441 gaagcgttag cggccagctg caacggagag cgcttgcgct cgtcctggag tggtggcatc
     4501 ttcatcgcac cgagctgctc gaggactggc accttgcaca agctggacaa tccctgaaga
     4561 agatcgatcc gctggactaa ctcacatgta ttacacggtc atagacgcac gatacgttca
     4621 cgatttcacg atctgggcaa agttcgccga cggaattgaa ggcgagatcg atctctccga
     4681 cgcgctctgg ggtccggtat tcgagccatt aaaggacgtg aactacttcc gcaacttctc
     4741 actcgccgag tacggcacga tctgctggcc gaacggtgcg gacatcgctc ctgagttttt
     4801 gtacggaaag gtacgggttc acgcgtgacg gtcgatgcta cgattacacc ggcgtagccc
     4861 ggcgccgtta ctccaggagt gaggagatat gaggaaatcg ctgggcatcg cgttcgtggt
     4921 tgcgttggtg tgtttgggtg ctttctccca gaccacgatc aggaatgatg ctgtgacgca
     4981 ggtgtcgccg gagggcgaca tcgtcgggtt tttcggccag cccgtcacgt tcgcgacaca
     5041 cgccgagctt gtgagctatg gattctcatt cggtccatcc gatggatcga tcggggcgat
     5101 tccagcggcg ggaggcacct atacgtttta tggcagtgcc ggctccacgg ctggctgcag
     5161 cgcaagtccg aacgtccacg gtacctttcc tttcaacgga acgctcgatc gtgttagcgg
     5221 agtcaccggt ggctgcagga ggatgtttgg tccaggagac gcgccggctg gctatgtgtt
     5281 cgacaaggac tatgcgggcg gtggcggggt tgttcgcttc gccagtggcg gcaagagcgg
     5341 atggctcatg cccttccatg ccgaatacca ttggcagaac caggccaacc caccgagttt
     5401 tttgtgcagc gcggggggaa acagtgcgtc catggtgccc tgcttttatt catcgatcgg
     5461 tctggccgta tcgacggacg acggaaagac cttccgagtg gcggggcaga tcgttcagcc
     5521 aagtgagccc ctgtctctgt tcgagggtgg cggcaccaac atggccgtcg gatacggctc
     5581 gcttctggta gccgacgcaa atggcaagca cctcgacaat ccaccgccgg acccggcgaa
     5641 cgcctacttc tatgtcttct ataacgatcg attgcagggc gctcccggcg cttgtagtaa
     5701 cgcgatctgc ttaggcgtcg ctcgtgcggt gtatgccgat gttgttacgg cggtgctctc
     5761 tggcgacggg caccaggtct cgcagttgtt tcacaagtac gatggagcgt cgccgaatcc
     5821 ctggactcag cccgcgactg cgttcgtgca gggaaacgcg acgcccgact tatcgggaac
     5881 ggccggcaag tacctgcctc tgtggaccga cgaaaacggg gcttccgcca tcgtgctcta
     5941 cgaccgcagc ttcgacgtct acctcgcggt ctacgtctcc ggcctcggcg tgtcgatcaa
     6001 ggcccgcgct tcgagcgaca tgatccattg gtctgagccg atcagtgctc catttaccga
     6061 ggcgggccgg gctctctttt acccgacgct cattggcgag acgggcgacc caacgatcgg
     6121 agggctcgcg ccgcgagtgt atttcaccag ctttcccacc gaccagtttc ccaactactc
     6181 gacctcggtc tttgaaagcg tcaccctgac gctggcaaga cagcggagac gcgcaacgaa
     6241 gcactgacga tgtcctgcgt cgtgtgcctt tgcgtcgtac gatgaggcgc atgaaggccc
     6301 gtcatctctt cgcgctctct ctggctctgg ccctcctgat cgcaaacagc gcgtttgccg
     6361 cgcagtctct cgattgcttc tatgcagctc gtcccaccgc gaagaacccc gatccggaga
     6421 tgagcctcca tcggaattgc gcggtcgtca gcggcgatgg ttccgttcgc atcgtggcgc
     6481 ggcatctcca ggcactcgac ttcaacgccg atggactggc cacgttcaac gccgagcgac
     6541 gcggttggtt ctatgtcctg cgcaatgggc gcattctcga agtgctgtcg gaagacaacg
     6601 gcgctgacga cttcgtcgaa ggtctggtgc gcgggcgccg tcatggaaag gtggcgttct
     6661 tcgatcgctc gtttcggatg gtgttgccgc ccatctacga tttcgcgtgg ccgttcgaga
     6721 acggtctggc catggtttgt tccggttgca cgcaaagaac cgacgcgtcc gatcccgatc
     6781 atcacggcct ggtggatggc ggtctctggg gctacatcga tcatcacggc agagaggtca
     6841 tccctgtgaa gtactcgcgc gacgaagcgc tgcggcagga agggctgctg tcaaagcgct
     6901 gaaagaaaat tacaaacttg ccccacaatg cggcctatgt ccaatcgcat ccgtgctctc
     6961 ctgctcctga tcgcaaccct cgcgacgacg agcacatttg cagcggacat tcgatggcag
     7021 cctcagagaa cttgggtgtt cgtcgtcgga acattggagt ggaacgattc cgaaacgttt
     7081 gcttcgtttc cgaaggcgaa ccgaagggat gcccagctcg tgcgcttttt tcgtcagaag
     7141 ggagtgaccg cgaatcacat cgtctacctc gcggacaaac aggccacgac gtcgcacgcg
     7201 caggcagcgc tcgacaggct gctcgcgcag acagctcccg gcgatctgct cttcttctat
     7261 ttcacagggc atggttatca atccgacgat gggaagaaga cgttctctgc gacgtatgac
     7321 gccagcgatc atcgggctgg atggaatgtc gatgcgatcg tgaggacgat cgccgccagg
     7381 tttcggggcg accgggtcat cctggctgcg gactgctgct actccggcgc gcttgtcgat
     7441 cgcgtgaagc ggctccgcgg ccgtcagtcg ttcgcgtgct tcgcgtcgtc gacgagcaaa
     7501 gacagctcga cggcgaactg gacgttcacg gagatgctgc tggccggact gcaaggtcg
//
LOCUS       DATLVW010000592         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_315344, whole genome shotgun sequence.
ACCESSION   DATLVW010000592 DATLVW010000000
VERSION     DATLVW010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315344"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /gene="parC"
                     /locus_tag="VNN25_17415"
     CDS             complement(<1..2048)
                     /gene="parC"
                     /locus_tag="VNN25_17415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXH93366.1"
                     /translation="MATRKSKEQTSFSPPPPIVPEVEHQPVAEFARKAYLDYSMYVIL
                     DRALPHVADGLKPVQRRIIYAMSELGLGPNAKPKKSARTVGDVIGKFHPHGDTACYDA
                     MVLMAQPFSMRYPLVEGQGNFGSPDDPKEFAAMRYTEAKLSRFAQSLLSELEQGTVDW
                     GQNFDGTLREPKLLPSRLPHVLLNGASGIAVGMATDIPSHNTREVVGACLELLDNPDA
                     TIAQLTKHIKGPDFPTEAEIITPLAEIREMYKTGNGSIRQRAKWEVENGDIVISALPF
                     MVSGSKVQQQIAAQMNAKKLPMIEDMRDESDHEDPTRLVLVPRSNRVDLDEVMAHLFA
                     TTDLERTYRVNMNMIGLNGRPRLYPLKDLLSEWLEFRIETVRRRLQHRYDHVNARIHI
                     LDGYLIAYLNIDEVIRIIRREDEPKPVLMKRFKLSELQAEAILELKLRFLNKLQEMEI
                     RGEQAKLEAERAELEKILGSKARLRKQVRDELVKDGEEFGDRRRSPIVEREAAKALDA
                     TALIPSEPVTVVLSERGWVRAGKGHDLDPTTLQYKSGDAFLHSARGRSNQLAVFIDSM
                     GRTYSLPAHELPSAKGHGEPLTAFTSPAPGATFVAVMMGDPEDLWLLSTDDGYGFIAK
                     LEDLITDRKAGKAVVNVSDGASVLRPQRMNRQETDRVAAATTSGKLLLFEAAELPLLA
                     R"
     gene            2229..2879
                     /locus_tag="VNN25_17420"
     CDS             2229..2879
                     /locus_tag="VNN25_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXH93367.1"
                     /translation="MLNWRARLREAIEKSGKKQSAIAADAGITPTTLSRILTAAHERP
                     TLDTVVRIARALDESVGWILDEPSFYLSADDQRKLHDAVRILDASLRSAGRRRTVRPE
                     SNAIAVTANREIPRAFAAAGARLIYEAAGDSMIGAAIADRDLLFVKPTNNIREASRKI
                     VVCRLGADEYVKHLDVRNGHIRLLSRNERHGPIEVGTGDEFELIGIVVGRAGAPVA"
BASE COUNT          578 a         1052 c          935 g          599 t
ORIGIN      
        1 cgagccagca acggcagctc ggccgcctcg aacagcagca actttccact cgtcgtggcc
       61 gcggcgacgc gatcggtttc ctggcggttc attcgctggg gccgcaggac ggatgctccg
      121 tcgctcacgt tcaccacggc cttgcccgct ttccggtcgg tgatcagatc ctcgagcttt
      181 gcgatgaaac cgtaaccgtc gtcggtcgag agcagccaca agtcttccgg atcgcccatc
      241 atcaccgcta cgaacgtcgc gcccggcgcg ggcgacgtga acgcggtcag cggttcgccg
      301 tgacctttcg ccgacggcag ctcgtgtgcc ggcagcgagt acgtgcggcc catcgagtcg
      361 atgaataccg caagctgatt gctgcgcccg cgcgccgaat ggaggaacgc atcgcctgac
      421 ttgtactgca gcgtcgtcgg atcgagatcg tggcctttgc ccgcgcgcac ccagccacgc
      481 tccgacaaca caaccgtcac cggctccgac ggaatcagcg ccgtggcatc gagcgcctta
      541 gccgcttcgc gctcgacgat cggcgagcgg cggcggtcgc cgaactcttc gccgtccttc
      601 accaactcat cgcgaacctg tttgcgcaac cgcgctttcg atccaaggat tttctcgagc
      661 tctgcgcgct cggcttcgag tttggcctgc tcaccgcgga tctccatctc ctgcagcttg
      721 ttcaggaatc gcagcttcag ttcgaggatc gcctcggcct gcagctccga caacttgaag
      781 cgcttcatca gcaccggctt cggctcgtcc tcgcggcgga tgatgcgaat cacctcatcg
      841 atgttgagat acgcgatgag gtagccgtcg aggatgtgga tgcgggcgtt gacgtgatcg
      901 taccgatgct gcaaccggcg ccgcaccgtc tcgatgcgga actccagcca ctcgctcagc
      961 agatccttta gcggatacaa ccgagggcgg ccgttgaggc cgatcatgtt catgttcacg
     1021 cggtacgtgc gctcgagatc cgtcgtggcg aagagatgcg ccatcacctc gtccagatcg
     1081 acacggttcg agcgcggcac gagcacgagt cgcgtcgggt cttcgtgatc cgactcgtcg
     1141 cgcatgtctt cgatcatcgg cagcttcttc gcgttcatct gcgccgcgat ctgctgctgc
     1201 actttcgacc ccgacaccat gaacggcagc gcgctgatga cgatgtcgcc gttctccact
     1261 tcccactttg cgcgttgccg gatcgagccg ttgcccgttt tgtacatctc ccggatctcg
     1321 gccagcggcg tgatgatctc cgcttctgtc gggaagtcgg gccctttgat gtgcttcgtc
     1381 agttgcgcga tcgtggcgtc aggattgtcg agcagctcca ggcacgcccc gacgacctcg
     1441 cgcgtgttgt gcgacggaat gtcagtggcc atgcctactg cgatccccga cgcgccgttg
     1501 agcagcacgt gcggcaaccg cgacggcagc aactttggct cgcgcaaggt gccgtcgaag
     1561 ttctgccccc agtcgaccgt cccctgttcc aactccgaca gcaacgattg cgcgaaccgc
     1621 gacaacttcg cctcggtgta ccgcatcgca gcaaactctt tcggatcgtc cggcgagccg
     1681 aagttccctt gcccttccac gagtggatag cgcatcgaga acggctgcgc catcagcacc
     1741 atcgcgtcgt agcaggcggt gtcgccgtgc ggatgaaact tgccgatgac gtcgccgacc
     1801 gtacgcgccg acttcttcgg cttcgcattc ggtcccaggc ccagctccga catcgcgtag
     1861 atgatgcggc gctggacggg cttgagacca tcggccacat gcggcagcgc gcggtcgagg
     1921 atgacgtaca tcgaatagtc gagatacgcc ttccgcgcaa actcggcgac aggctgatgc
     1981 tcgacctcag gaacgatggg cggcgggggt gagaaggaag tctgctcttt cgattttcgg
     2041 gtagccatca agtcctggcg cggcggaatt cagtgacacc tataggttac cgtacctgtt
     2101 ccgctcacgc gatcgaaaga atcgcctcag ggcctcgtcc tcgactacat ggccgaggta
     2161 ccctttcctg tgtggaaggc gatgcaaccc tgtgccttcg cttttggcga ccggcaacat
     2221 cagtaacagt gttgaattgg cgcgcccgcc tacgcgaagc aatcgagaag agcggcaaga
     2281 aacagagcgc catcgctgcc gacgccggta tcaccccgac gacgctcagc cggattctga
     2341 ccgcggcgca cgagcggccg acgctcgaca cggtcgtgcg catcgcccgc gcgttggacg
     2401 agagtgtcgg atggatcctc gatgagccga gcttctacct ctccgccgac gatcagcgaa
     2461 agctgcatga cgcagtgcgg attctcgacg cgtctctgcg cagcgccgga cggcggcgaa
     2521 cggtgcgtcc ggaatcgaac gccatcgccg taaccgcgaa ccgcgagatc ccgcgcgcct
     2581 tcgccgccgc cggtgcccga ttgatctacg aagccgccgg cgactccatg atcggcgccg
     2641 ccatcgccga ccgcgatctc ctattcgtca aaccgacgaa caacattcgc gaagcatccc
     2701 gcaagatcgt cgtctgccgc ctcggcgccg acgaatacgt caagcacctc gacgtgcgga
     2761 acggccacat ccgccttctc agccgcaacg agcgtcacgg cccgatcgaa gtcgggaccg
     2821 gtgacgaatt cgaattgatc ggaatcgtcg tcggccgtgc cggagcaccg gtggcatgag
     2881 cgcagcccga gaagtcaaac catttcgaca ccgcgaccga actaatcaag acttgcagcg
     2941 gcagtttgca acgctgttgt tactcgtcgc agccgtacag gacgcgtagt gtagtagcca
     3001 ccagtgcagg ttaccgagca ggaaccgtac gagtttgacg tcgagcagga ggtcgagcca
     3061 atccctccag agtcacacgt tccgcctccg cctggtcctc cccctcctcc aagaacgtcg
     3121 taatcggcgt tgtcaaggca aacgctgaag tcggagcagt catc
//
LOCUS       DATLVW010000593        12999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_235216, whole genome shotgun sequence.
ACCESSION   DATLVW010000593 DATLVW010000000
VERSION     DATLVW010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235216"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VNN25_17425"
     CDS             <1..1251
                     /locus_tag="VNN25_17425"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93368.1"
                     /translation="RNWMENQPKMVRTLADLLGASKERLVSAKRKPRPSAPSVEDVYA
                     LAGCGRVLPAKAGTPPSELQLTPGHATTLRGHLGKTLGYLNRFSGGAIFLAAADLLGS
                     TSIGEAGKDFQAGFFNCVSNSDSRTLSIGGICEDAMAAVLSGVSSFGKHIGAGSSYAA
                     FLAPLGHIAARLHAIGNQARNEATGEAARPMILVCAHAGLATGEDGPTHADPQPLQLL
                     QENFPPGAMITLTPWDAQEIWPLLTAAIARRPSVIAPFVTRPPQRVVDREALGLAPAA
                     HAAQGVYCLREAPGRCDATIVLQESGVTYAFVTETLPLLDRAGIAVDVFYVASAELFD
                     SLPEEERDAIFPEARQQLAMGITGFTLPTMYRWIRSDRGRAMTMHPFMHGHYTGSGPA
                     RAVIAEAGLDGASQFRRIAEYVRA"
     gene            complement(1651..2202)
                     /locus_tag="VNN25_17430"
     CDS             complement(1651..2202)
                     /locus_tag="VNN25_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93369.1"
                     /translation="MSIAAIAILVVAAAVLSGLLVCRFMVYRGQRVIICPETQQPAGV
                     ELNAWRAARTPGDANLRLQSCSRWPERHDCPQDCLAQIEASPDGTLVRNIVAAWYGNQ
                     QCVFCHRTIGTIEWHEAIPALRASDGTLKEWKGIAAEDVPAILETHNAVCWNCNLTEG
                     FRHEHPELVTDSAQTPLRDLAIH"
     gene            complement(2278..3156)
                     /locus_tag="VNN25_17435"
     CDS             complement(2278..3156)
                     /locus_tag="VNN25_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93370.1"
                     /translation="MLWLAATLFAAATALHRPQPEVLFAVQPDGDSQGDFSPDKLAIE
                     PVAMITKLPGSCPGCLKLRITAPPRDADDEFEARYFRPGRTYRVLDGGAEIGTVKVTK
                     LTSLGCVSLAASVDVISRSNWDPQLLSVLAVGSLHLPPVEPVRRVPDDAEDMALRKIA
                     EHELGAKGVSASLLAKMEGGAAISADLDHDGRRDLIGSFSTNDGTPQRRTYNLFLIAM
                     GDESGGFRAEHVWYFTYLGDGGEGQDVVDTIDLDEDGTDEIVVRSRFIESHEYEILKR
                     AGNKWKSVYQGGGSGC"
     gene            complement(3157..3555)
                     /locus_tag="VNN25_17440"
     CDS             complement(3157..3555)
                     /locus_tag="VNN25_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXH93371.1"
                     /translation="MTRRRADGYVMISVIAERYNIHPQTLRLYEREGLVKPARTSGNT
                     RLYGESEIRKLEMILALTRDLGVNLAGIEVILNMQRALEDAREALDRADGHFDDMMRV
                     IRQAVLDKRHGIERENALVKAVPRGLTRIK"
     gene            complement(3610..5541)
                     /gene="dnaK"
                     /locus_tag="VNN25_17445"
     CDS             complement(3610..5541)
                     /gene="dnaK"
                     /locus_tag="VNN25_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXH93372.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGNEPKVIVNAEGSRITPSVVGFAR
                     NGERLVGQVAKRQAVTNPENTIFSIKRFMGRKYEEVNEEMKMVPYTVTRASNGDARVE
                     VDGKLYSPPEISAMILQKLKQAAEDYLGEKVDRAVITVPAYFNDSQRQATKDAGEIAG
                     LKVERLVNEPTAAALAYGLDKKKNETIAVYDFGGGTFDISILEVGDGVVEVKSTNGDT
                     HLGGDNIDHRLIDWIIEEFRKDQGIDLSKDKMALQRLKEAAEKAKIELSSTMSTDLNL
                     PFITADQSGPKHLNMSLTRAKFEQLIGDIVQKTMGPLKQALNDAGVDPKKIDEVVLVG
                     GSTRIPMVQKIVTDFFGKEPHKGVNPDEVVAVGAAVQGGVLAGDAGLKDVVLLDVTPL
                     SLGIETLGGVMTKLIERNTTIPTRKTETFSTAADNQTSVEIKVIQGEREMARDNKLLG
                     VFHLIGLPPAPRGMPQIEVTFDIDANGIMTVSAKDTGTGKEQKITITSTSGLSKDEVE
                     KLVRDAESHAAEDKARREEIEAKNQLDSLVYQVEKMLNENRDKISGSDVSNLESAIAD
                     ARKAMEQGGADNIKKATETLTTASHKLAEVMYQNASANAAGGAGGGAGAAGGSSAEAP
                     EGNGKADDVIDAEVVDENK"
     gene            5716..5943
                     /locus_tag="VNN25_17450"
     CDS             5716..5943
                     /locus_tag="VNN25_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HXH93373.1"
                     /translation="MRTTLDIDQDVLQAAKEIAINRGTTAGKVLSDFARKGMSSGHEA
                     QVRNGVPLIPRRPEGSPRPTMDTVNALRDEK"
     gene            6090..6401
                     /locus_tag="VNN25_17455"
     CDS             6090..6401
                     /locus_tag="VNN25_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93374.1"
                     /translation="MVRILSNIAYTQGRHESTEAVRNRLDTFCASGNHSFWPEQVSIR
                     DEQRFKLSGLSHGKITDVYLLAIAVDQGGRLVTFDRRIPLTAAVGAASDQLEVIEVQA
                     N"
     gene            complement(6581..6913)
                     /locus_tag="VNN25_17460"
     CDS             complement(6581..6913)
                     /locus_tag="VNN25_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93375.1"
                     /translation="MAKPDEGGTAARAMTGNVAGGIISVGARKILLLGEKVLLRDQKV
                     LLQEEKILLGEEKILLQEEMKLLQEEMKVLGEGKVFHQEEKVSLLEQILLLREQMLLL
                     EEDKVLLQ"
     gene            complement(7022..7336)
                     /locus_tag="VNN25_17465"
     CDS             complement(7022..7336)
                     /locus_tag="VNN25_17465"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXH93376.1"
                     /translation="MPQSPQVYQLARYEVRPESLDRCLTAIHQFVAYVREHEDGTLRY
                     DVWQERDHPTRFVHQFIFRDAAAEQTHSDSAEVKQFASVLYPECLAPVDFIDYDLVTS
                     KS"
     gene            complement(7353..8114)
                     /locus_tag="VNN25_17470"
     CDS             complement(7353..8114)
                     /locus_tag="VNN25_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXH93377.1"
                     /translation="MRRFLLWYLPLTAALAAAALFGAGFYSFLRGDTGTSVSLMPSTT
                     RITASAPRKQIAPIIVGDSLGRGTGDETGLGIGGRFVDELRKRNIDTKNIVNLAVNGA
                     RTRDLIRQLDSHSVQVLLAQSNVIIVSIGGNDLWGDNTFRNAPPPNPVGVMREVLDRV
                     DQSVRTIRTANPTARIFVIGLYNPFSSTPYGRMLTPFVNRWNSLLVERFASDANVVVV
                     QTSDIFAYRDRLSFDRFHPGEEGYSLIARRIADAI"
     gene            8149..9060
                     /locus_tag="VNN25_17475"
     CDS             8149..9060
                     /locus_tag="VNN25_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93378.1"
                     /translation="MSETPTLTASDLTKTIGHKTIVDSVSFELQQGEVFGFLGPNGAG
                     KTTTIRMLVGLIRPTAGSVRICGYDIQRNFEDAMRCVGCIVENPDLYRFMTGRENLEH
                     FARMLRVPGSEIERVAEAVSLAHRLEQRVGTYSLGMRQRLGIAQALLGNPRLLILDEP
                     ANGLDPAGIREIRELLRRLASEQQMSIFISSHLLAEIELTCDRVAIIHKGRILREGTV
                     RDLISSRRDMELRVDDVPRASAIARERNLTFRTDGDRIWLAMEEADAPPLLSALAAAG
                     VGIFHAQRRAQSLEEMFLEATGGETVD"
     gene            9070..10041
                     /locus_tag="VNN25_17480"
     CDS             9070..10041
                     /locus_tag="VNN25_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93379.1"
                     /translation="MLIPLIQNETLKIVRRKRFAVVIAILFVILALVTYSQVRRLRDQ
                     QNRNWRAEIQQRVANFQNMMRRGRMNEGYARGLRAEIGRLQFYLDHDIEPDKPTAPLF
                     VRGFANASGWLLPLLVAMFGADIVSAENAEGTDKLLLTRPVRRWKILTAKLLTLFMFA
                     TLTLLCGAVIAYAISSPFLPSGGWNAPTFVGFQLNGNSVSLDAVRQLPLWKDTMIAYG
                     LQWYALLAVAAIALLLSVLFRSSAAAIGTMLASLIGGTILTRISPDWTAGKYLFVSAL
                     PLADYYTGQPPPYDGMSMLFCIGLLGAWAIGSTIISYAVFTRRDVFG"
     gene            10041..10604
                     /locus_tag="VNN25_17485"
     CDS             10041..10604
                     /locus_tag="VNN25_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXH93380.1"
                     /translation="MRIRFNVAPILALALFCFACGPEPSKVVTDDRMPIAVEYVRGAT
                     MTIHTKPADDAPVISTYQRGESVSVLARKGEWAEVRTGANSGWARIAEMADAAEAKKA
                     LEDNPWPHFTRTPPPVSQPGAHGELVLEAEVDQDGIVKSVRTLSNTTGSMSLEQRNTE
                     ALRNAEFTPVVVKGKRKAFVYEHRVHY"
     gene            complement(10897..11478)
                     /locus_tag="VNN25_17490"
     CDS             complement(10897..11478)
                     /locus_tag="VNN25_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXH93381.1"
                     /translation="MRSLCVFCGSSAGNDPRFAQAASELGALLAKEGITLVYGGGHVG
                     LMGILADAVLANGGKVIGVIPRALWDREVGHRNLTELRVVETMHERKALMASLADAFV
                     ALPGGLGTLEEIFEVWTWSQLGIHDKPVGFLDVDGFYAPLLAFLDHGVDAGFVRAPHR
                     AMAIVDGDGESLLRRMDEYEPPKVTKWISAQAT"
     gene            complement(11478..11717)
                     /locus_tag="VNN25_17495"
     CDS             complement(11478..11717)
                     /locus_tag="VNN25_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93382.1"
                     /translation="MPCDHIGPSELIDMAEAQLRERGVRPEAGMRFRWSENPADGMWA
                     SIVTEIERRGEQWIVTRLDRNREPLPVDETGFRAL"
     gene            11914..>12999
                     /locus_tag="VNN25_17500"
     CDS             11914..>12999
                     /locus_tag="VNN25_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXH93383.1"
                     /translation="MPQFRYDIRNVAIIAHVDHGKTTLVDALLRQAGAVRLNQQMDER
                     VMDSNDLEKERGITILAKNTSIRYLVDEPDALGHLVHHEATHTASGHDLPAAFIHPSE
                     VKINIVDTPGHADFGGEVERVLSMVDGVILLVDAAEGPMPQTRFVMRKALGLGLLPIV
                     VINKIDRQDARPTEVVNEVFDLMIELGASDEQLDFPILYAIGRQGIVRTNLEDTTEDC
                     RPLFDAILKRIPPPPGYADAPLQLLVSAIDYNEYVGRLGVGRIQRGKLRQGEDVVLIL
                     RDGTHKKGRITKLTAFEGLRRVEVEEASAGEIVAVAGFTDVEIGETFSSAANPEALEP
                     IAIDEPTVSMFWLVNDSPFAGTEGKYVT"
BASE COUNT         2397 a         4249 c         3848 g         2505 t
ORIGIN      
        1 cgcaactgga tggagaacca gccgaagatg gtgcggacac tggcggacct cctcggtgct
       61 tccaaggaac ggctcgtctc cgcgaaacgg aagccgcggc cgtccgctcc atccgtcgaa
      121 gacgtctacg cactcgcggg ctgtggccgc gtcctccctg cgaaggccgg cactccgcca
      181 tccgagcttc agctcacgcc cggacacgcc actacgctcc gcggacacct cggcaagacc
      241 ctcggatacc tcaatcgctt cagcggcggc gcgatcttcc tggcagccgc ggaccttctc
      301 ggttcgacca gcatcggcga agcgggaaag gattttcagg caggattctt caactgtgtc
      361 agcaacagcg actcgcgaac gctctcgatc ggcggcatct gcgaagacgc gatggccgcc
      421 gtgctctccg gcgtctcgtc cttcggaaaa cacatcggcg ccggctcgtc gtacgccgcc
      481 ttcctcgcgc cgctcggaca tatcgccgcg cggttgcatg cgatcggaaa tcaggcccgc
      541 aacgaggcga cgggagaagc ggcccggccg atgatcctgg tctgtgccca tgccgggctg
      601 gccaccggcg aggatggacc gacgcatgct gatccgcagc cgctgcagtt gctgcaggag
      661 aacttcccgc cgggcgcgat gatcacgctg acaccgtggg acgcgcagga aatctggccg
      721 cttcttactg ctgcgattgc gcggcgccca tccgtgatcg caccgttcgt cacgcggcct
      781 ccgcaaaggg tcgtcgatcg cgaagccctc ggcctggcgc ccgcggctca tgcggcgcaa
      841 ggagtctatt gtcttcgcga ggcgccggga cgatgtgacg cgaccatcgt gctgcaggaa
      901 tcgggcgtga cgtatgcgtt cgtgacggag actctgccgc tgctcgatcg cgccggaatc
      961 gccgtcgacg tcttctatgt cgccagtgcg gagctcttcg attcactgcc ggaggaggag
     1021 cgcgacgcca tcttcccgga ggctcggcaa caattggcca tgggtatcac cggcttcacg
     1081 ctgccgacga tgtaccgctg gatccgatcc gaccgcggac gggccatgac gatgcatccg
     1141 ttcatgcacg gccactacac gggcagcggc ccggcgcgtg ccgtgatcgc ggaagcgggt
     1201 ctcgatggcg ccagccagtt ccgcagaatc gcagaatacg tgcgcgcttg acgcctgcgg
     1261 gaccggttcg gactgaagtg tcggctggct tacgcgcaac tcgacggatc gcgttcgcga
     1321 ttaactcgca ttcctcacac ctttggtggc ctccttgcta caactcttcc gctttctttg
     1381 gagtgccttc cccatcgagc gccttctccc cccgcgcttt ttgcgggggg agaaggtgcc
     1441 gaaggcggat gagggggagc acttgcgcga ggcgatcaac cgagcctcgt ccgaactgcc
     1501 tgaaaacgcg gcacgtgcac ggatgtcgcg ctggttggga cgccccctca tccggcttcg
     1561 ccaccttctc ccccactgag aagcgtgggg gagaaggcgc tcgattgaaa caccctgcca
     1621 gcaattccct gcaagtgtga gaaatgcaag ctagtgaatg gccaggtcgc gcaacggcgt
     1681 ctgcgcgcta tcggtcacga gctctggatg ctcgtgccgg aacccctcgg tgaggttgca
     1741 attccagcag acagcattgt gggtctcgag aatcgccggg acgtcttccg ccgcgatccc
     1801 cttccattcc ttcagcgttc cgtcggatgc acggagcgcg gggatcgctt catgccattc
     1861 gatcgtaccg atcgtgcgat gacagaagac acattgctga ttgccgtacc acgccgcgac
     1921 gatgttccgc accagcgttc cgtccggcga cgcttcgatc tgcgccaggc aatcctgcgg
     1981 acaatcgtgc cgctcgggcc agcgtgagca gctttgcaga cgaaggttcg cgtcgcccgg
     2041 agtgcgcgct gcccgccagg cattgagctc cacgcctgcg ggctgctgcg tttcgggaca
     2101 gatgatcacg cgctgtccgc gatagaccat gaatcgacag acaagaagac ccgacagaac
     2161 tgcagcggcg acgacgagaa tggcaatggc agcgatgctc atagggcaac ggtggccctc
     2221 ccgttacccg gttgctgcga taacaatcac acagccgtgt gacgaacgat cgcgagctta
     2281 gcagcccgat ccgccgcctt ggtagacgct cttccacttg ttaccggcgc gcttcaggat
     2341 ctcgtattcg tgcgactcga tgaagcggga acggacgacg atctcgtcgg tgccgtcctc
     2401 atcgagatcg atcgtatcaa cgacatcttg gccttctccg ccatcgccca agtaggtgaa
     2461 ataccagacg tgctcggcgc ggaatccgcc ggactcatcg cccatggcaa tcagaaagag
     2521 gttgtacgtc ctcctctgcg gtgtaccgtc attggtcgag aaagaaccga tcaggtcgcg
     2581 tcgaccgtcg tgatccagat cagcggagat agcggctccc ccctccatct tcgcaagcag
     2641 tgacgcagaa acacctttgg cccccagctc gtgctcggca atcttccgga gcgccatatc
     2701 ctccgcgtca tccggaacgc ggcggaccgg ctctaccggc gggagatgca gcgaaccgac
     2761 agcaagtacg ctcagcagtt gcggatccca gttcgaccgc gatatgacgt cgacactcgc
     2821 cgccaacgat acgcagccta gactcgtcag cttggtcacc ttcactgttc cgatttcggc
     2881 gccgccgtcg agcacgcgat aggtgcggcc cgggcggaaa tagcgcgcct cgaactcatc
     2941 atcagcgtcc cggggtggtg cagtgatgcg cagcttcagg catccggggc aggaaccggg
     3001 cagcttcgtg atcatcgcga cgggctcgat ggccagtttg tccggcgaga agtctccctg
     3061 cgagtcgccg tctggctgga ccgcgaagag aacctccggc tgcgggcgat gcagcgccgt
     3121 tgcagcggcg aagagcgtgg cggcaagcca gagcatttac ttgattctcg tcaatcctcg
     3181 gggaaccgct ttcacgagcg cgttctcgcg ctcgatgccg tgacgcttat cgagaacggc
     3241 ctggcggatg acgcgcatca tatcgtcgaa gtgaccgtcg gcgcggtcga gcgcctcgcg
     3301 tgcgtcctcg agggcgcgtt gcatgttgag gatgacttcg atgccggcca ggttcacgcc
     3361 gaggtcgcgg gtgagggcca gaatcatctc cagcttgcga atctcgctct cgccgtagag
     3421 ccgcgtattg cccgacgtcc gggccggctt gaccagccct tcgcgctcgt aaaggcgcag
     3481 cgtctgcgga tggatgttgt atcgctcggc gatcaccgag atcatcacgt atccatctgc
     3541 tctgcggcgt gtcatttgtg gttccagcaa caaaagaggg cgacggtttc ccgccgccct
     3601 cggatcagct tacttgtttt cgtccacgac ttcggcgtca atcacgtcgt ccgccttgcc
     3661 gttgccttcc ggagcttcgg ccgacgaacc gccggccgct cctgctcccc cgcccgcgcc
     3721 gcccgcggca ttcgcgctcg cgttctggta catcacttcc gcgagtttgt gcgacgccgt
     3781 cgtcagcgtt tccgttgcct tcttgatgtt gtccgctccg ccctgctcca tcgctttgcg
     3841 ggcgtcggca atcgcgcttt cgaggttgct cacatccgag cccgagatct tgtcgcggtt
     3901 ctcgttgagc atcttctcga cctggtagac gagcgagtcg agctggttct tcgcctcgat
     3961 ctcctcacgc cgcgctttgt cctcggccgc gtgcgactcg gcgtcgcgca ccagcttctc
     4021 gacttcatcc ttcgacagac ccgaggtgct ggtgatggtg atcttctgct ccttgccggt
     4081 gccggtgtct ttcgccgaga ccgtcatgat gccgtttgca tcgatgtcga atgtcacttc
     4141 gatctgcggc atccctcggg gcgcaggcgg tagtccgatc aggtggaaca cgccgagcag
     4201 cttgttgtcg cgcgccatct cgcgctcgcc ctggatgact ttgatctcga ccgacgtctg
     4261 gttgtccgcc gccgtcgaga acgtctccgt cttgcgcgtc gggatggtcg tgttgcgctc
     4321 gatcagcttc gtcatcacgc cgcccagcgt ctcgattccg agcgagagag gcgtgacgtc
     4381 gagcaggacg acatccttga ggcctgcgtc gccggcgagc acaccgccct gaaccgcggc
     4441 accgactgcc acgacttcat ccggattcac gcccttgtgc ggctcttttc cgaagaagtc
     4501 ggtgacgatc ttctggacca tcgggatgcg cgtcgaaccg ccgacgagga cgacttcatc
     4561 gatcttcttg ggatcgacgc cggcgtcgtt gagcgcctgc ttgagcggtc ccatcgtctt
     4621 ctgcacgatg tcgccgatga gctgctcgaa cttggcgcgg gtgagcgaca tgttgaggtg
     4681 cttcgggccg gactgatcgg ccgtgatgaa cgggagattg aggtcggtcg acatggtcga
     4741 cgaaagctcg atcttcgctt tctcagcggc ttccttcaga cgctgcagag ccatcttgtc
     4801 tttcgacaga tcgatgccct ggtctttgcg gaactcttcg atgatccagt cgatgaggcg
     4861 gtgatcgatg ttatcgccgc cgaggtgcgt gtcaccgttg gtcgacttca cttcgacgac
     4921 gccgtcgccg acttcgagga tcgagatgtc gaacgtgccg ccgccgaagt catacacggc
     4981 gatcgtctcg ttctttttct tgtcgaggcc gtatgcgagc gcggccgcgg tcggctcgtt
     5041 gacgaggcgc tcgaccttga ggccggcgat ctcgcccgcg tctttcgtcg cctggcgctg
     5101 cgaatcgttg aagtaggccg gaacggtgat gaccgcgcgg tcgaccttct cgccaaggta
     5161 gtcctcagca gcctgcttga gcttctgcag aatcatcgcg gaaatctcgg gcggcgagta
     5221 gagcttgccg tccacctcga cacgcgcgtc gccgttcgag gcacgagtga ccgtgtaggg
     5281 gaccatcttc atctcttcat tgacttcctc gtacttccgc cccatgaagc gcttgatcga
     5341 gaagatggtg ttctcaggat tggtaactgc ctggcgcttt gcgacctggc cgacgagacg
     5401 ctcgccgttt ctggcaaatc cgaccaccga gggggtgatc cggctgcctt cggcgttgac
     5461 gatgactttg ggctcgttgc cctccatgac cgccacgacg gagttggtcg tgccgaggtc
     5521 gataccgata atcttggaca tatgtttttc tccttctgat acttaccgca acatgagtgc
     5581 gatgcagtca gataatctgc cagacaaaac ctgagtgcgc cggtgtgata ttccgcacag
     5641 tttagtgcaa gtttggtgcg cgtcagcgca taatctccgg ttgacatctg atgcgtgatg
     5701 atcgacgatc tcgagatgag aaccacacta gatattgatc aagacgtcct acaggcagcc
     5761 aaagagatcg ccatcaatcg cggaacgacc gcgggaaaag ttctgtcgga cttcgcgcgg
     5821 aaggggatgt cgtccggtca tgaggcccaa gtgcggaacg gcgttccgct gatccctcgg
     5881 cgacccgaag gcagcccgcg tccaacgatg gacactgtga acgctcttcg cgacgagaag
     5941 taagcgacga tggctccagt cgctcttcta gatgtcaacg ttctggtggc tctcttcgac
     6001 caggaccaca ttcatcatga agccgcccac atttggtttt ccgcgaaccg cagccgggct
     6061 gggcgacctg cccgatcact gaaaacggcg tggttcgcat cctctcgaat attgcgtaca
     6121 cgcaaggccg gcacgagagc accgaggcag ttcggaaccg tctggatact ttctgtgcga
     6181 gcggcaatca ctcattctgg cccgagcagg tatcgatccg cgacgagcag cgcttcaaac
     6241 tttccgggtt gagtcatggc aagatcaccg atgtctacct tctcgcgatc gccgtcgatc
     6301 agggtggacg gctcgtgacg ttcgatcgcc gcattccgct gacggccgcc gtcggcgccg
     6361 cgtcagatca actcgaggtt atcgaggtcc aagcgaacta gcgattctgc agagccaacg
     6421 gttgcgttct ttcttcgaat gaggagattt ttcaggttcc gcgcaaaact tttctgatca
     6481 ggaccaaaat cttctgctcc tggagcacga ctttgtggtc ccggaggaaa actttttgct
     6541 cctggagcaa atgcatttgc tcttggatgg aaactttttc ctactggagc aaaactttgt
     6601 cctcctcgag caaaagcatt tgctcccgga gcaaaagtat ttgctctagg agggaaactt
     6661 tttcctcctg gtggaaaact tttccctccc cgagcacttt catttcctcc tggagcagtt
     6721 tcatttcctc ctggagcaaa atcttttcct ccccgagcag aatcttttcc tcctggagca
     6781 aaactttttg atcccggagc agaactttct cgccgaggag cagaatcttt cgcgcgccga
     6841 ctgagatgat gccgccggcg acattgcctg tcatcgcgcg ggctgctgtt cccccctcat
     6901 ccggcttcgc caccttctcc ccccgcaaaa agcgcggggg gagaaggcgc tcgattgggg
     6961 ttcgcggctg aaaaacgaca gtgcttacga cttcgtcgac gtcaccacaa cttcgacgtc
     7021 actacgactt cgacgtcaca aggtcgtagt cgatgaagtc gaccggcgcc agacactcgg
     7081 gatagagcac cgaggcgaac tgcttgacct ccgccgaatc ggagtgagtc tgctcggccg
     7141 cggcgtcccg gaagatgaac tggtgaacga agcgcgtcgg atgatcgcgc tcctgccaca
     7201 cgtcgtagcg caacgttccg tcctcgtgct cgcgcacgta cgcgacgaac tggtggatgg
     7261 cagtcagaca gcgatcgagc gattccggac gaacctcata gcgagcgagt tgatagacct
     7321 gcggtgactg tggcatctga cctccctaat cgttaaatgg cgtcggcaat ccggcgcgcg
     7381 atcagcgaat acccttcctc gccaggatgg aaccggtcga acgagagccg gtcccgatac
     7441 gcgaaaatgt cggaagtctg cacgacgacg acgttcgcat cgctcgcgaa gcgttccacc
     7501 agcagcgagt tccagcggtt cacgaacggt gtgagcatcc tcccgtacgg cgtgctggag
     7561 aacgggttgt agagcccgat cacgaagatg cgcgccgtcg gattcgcggt tcgaatcgtt
     7621 cgcacgctct gatcgacgcg atcgagcacc tcgcgcatga caccgaccgg gttcggcggt
     7681 ggcgcattgc gaaaggtgtt gtctccccaa aggtcgttgc cgccgatgga gacgatgatg
     7741 acgttcgatt gcgcgagcaa cacctggacg ctgtgactgt cgagctgccg gatcagatcg
     7801 cgcgtccgcg cgccgttgac cgcgaggttg acgatgttct tcgtgtcgat gttgcgcttc
     7861 cgcaactcgt cgacaaaccg tccgccgatg cccagcccag tctcgtcccc ggtgccacgt
     7921 cctaacgaat cgccgacgat gattggtgcg atctgcttcc gcggcgcgga tgccgtgatc
     7981 ctcgtcgtcg agggcatcag tgacacggat gtaccagtgt cgccgcgcag gaatgagtag
     8041 aatccggctc cgaacaaggc cgccgcagcc agggcggcgg tcagaggcag ataccacaag
     8101 aggaaacggc gcattgcgtg aaacgataac aggaatgaga ctcattcgat gagtgagacc
     8161 cccaccctca cggcctcgga tctgaccaag accatcggtc acaagacgat cgtcgactcg
     8221 gtctcattcg agcttcagca aggcgaggtc ttcggcttcc tcggaccgaa cggggcaggc
     8281 aagacgacga cgatccgcat gctggtcggc ctcatccgtc caaccgcagg gagcgtgcgg
     8341 atctgcggct acgacatcca gcgcaacttc gaagacgcca tgcgttgcgt cggctgcatc
     8401 gtcgagaacc ccgacctcta tcgcttcatg accgggcgcg agaacctcga gcacttcgcg
     8461 cgcatgcttc gcgttccggg tagcgagatc gagcgcgtcg cggaagccgt ctccctcgcc
     8521 caccgcctcg agcagcgcgt cggcacctac tccctcggca tgcgccagcg tctcggcatc
     8581 gcccaggcgc tcctcggcaa cccgaggctc ctcatcctcg acgagccggc caacggcctc
     8641 gatcccgccg gcatccgcga gatccgcgaa ctgctgcggc gcctcgccag cgagcagcag
     8701 atgagcatct tcatctcctc acatctcctc gccgagatcg agctcacctg cgaccgcgtg
     8761 gccatcatcc acaaaggacg catcctccgc gaaggcacgg tgcgcgatct gatctccagc
     8821 cgccgcgaca tggagcttcg cgtcgacgac gtcccgcgcg cgtccgcgat cgcacgcgag
     8881 cgcaacctca cctttcgtac cgacggcgac cgcatctggc tggcgatgga ggaagctgat
     8941 gccccgcctc tcctctccgc gctcgccgcg gcaggcgtcg ggatcttcca cgcccaacga
     9001 cgggcgcaat cgctcgaaga gatgttcctc gaagccaccg gcggcgagac ggtagactga
     9061 gacgcgcgaa tgctcatccc cctcattcag aacgaaacgc tgaagatcgt ccgccgcaag
     9121 cgcttcgcgg tggtgattgc gatcctcttc gtgattctgg cgctcgtcac ctactcgcag
     9181 gtccggcggc tgcgcgacca gcaaaatcgc aactggcgcg ccgagattca gcagcgggtc
     9241 gcgaacttcc agaacatgat gcgccgagga cggatgaacg aaggctatgc gcgcgggcta
     9301 cgcgcggaaa tcggccgcct gcagttctat ctcgatcacg acatcgagcc ggacaagccg
     9361 acggcgcctc ttttcgtgcg cggcttcgcc aacgcctcgg gatggctgct gccgctgctc
     9421 gtagcgatgt tcggtgccga catcgtctcc gcggaaaacg cagaaggcac ggacaagctg
     9481 cttctgacac gcccagtgcg ccgctggaag atcctcaccg caaaactgct gacgctcttc
     9541 atgttcgcga cgctgacgtt gctctgcggc gcagtcatcg cttacgccat ctcttcccct
     9601 ttcctcccct cgggcggctg gaacgcgccg acgttcgtcg gctttcagct gaacggtaac
     9661 agcgtcagcc tcgatgcggt acgccaactg ccgctctgga aagacacgat gatcgcgtat
     9721 ggactgcagt ggtacgccct gctcgccgtt gccgcgatcg cgttgctgct gtcggttctc
     9781 ttcaggtcga gcgccgcagc gatcggaacg atgctggcct cgctcatcgg cggcacgatc
     9841 ctcacgcgca tctcccccga ctggaccgcg ggcaagtacc tcttcgtcag cgcactcccg
     9901 ctggccgatt actacaccgg ccagccgccc ccctacgacg gcatgtcgat gctgttctgc
     9961 atcggcctcc tcggcgcgtg ggcgatcggg tcgacgatca tctcctacgc ggtattcacg
    10021 cgaagggacg tgttcggatg atgcgcattc gattcaacgt ggcgccgatc ctcgcactcg
    10081 cgctcttctg cttcgcctgc ggacccgagc cttcgaaggt agtcaccgac gacaggatgc
    10141 ccatcgccgt cgaatacgtc cgcggcgcga cgatgacaat tcacacgaag ccggccgacg
    10201 acgcgccggt catcagcacg taccagcgcg gcgaatcggt gtctgtactt gcgcgcaaag
    10261 gcgaatgggc cgaggtgcgc acgggtgcga acagcggatg ggcccgcatc gctgaaatgg
    10321 ccgatgccgc cgaggcaaag aaagcgctcg aggacaatcc gtggccgcac ttcacacgca
    10381 cgccgccgcc ggtctcccag cccggcgcgc acggagagct ggtactggaa gccgaagtcg
    10441 atcaggacgg aatcgtgaag agcgtccgca cgctcagcaa cacgaccggc tcgatgtcgc
    10501 tcgaacagcg caacaccgag gccctgcgca acgccgaatt cacgccggtc gtggtcaagg
    10561 gcaagcgcaa agcgttcgtg tacgagcatc gcgtgcacta ttgaggcgcg ttctggttct
    10621 accacgcgcc tggtgtgttg ggtgtcatcc tgagctgccg gaggcgcccg cgttactcga
    10681 cgctctcgcg cagaaactcg ccggcgccga aggacagcga aggatctcta attacgatgc
    10741 acatgacatg tgcgcggcaa cttgagatcc ttcgccgtcc tacgccgcca catcgcgctg
    10801 cgcaaattga tcgagcacgc ggcggctccg gctgtctcag gatgacacat caagttgatg
    10861 acacgcggaa tggcagcgac ggtgacaacg aatcgctcac gttgcctgcg cgctgatcca
    10921 cttcgttacc ttcggcggct cgtactcgtc catccgccgc agcagcgatt ctccatcccc
    10981 atcgacgatc gccatcgccc ggtgcggcgc acgaacaaag cctgcgtcca ccccgtgatc
    11041 gagaaacgcc aggagcggag catagaaccc gtcgacatcg agaaagccaa ccggcttgtc
    11101 gtggatgccg agctgcgacc acgtccagac ttcaaagatc tcttccaacg tccccagccc
    11161 gcccggcagg gcaacgaagg catccgcgag cgacgccatc agcgccttgc gctcgtgcat
    11221 cgtctcgacg acgcgcagct cagtgagatt gcgatgaccg acttcgcgat cccacaacgc
    11281 tcgaggaatg acgccgatga ctttgccgcc gttcgcgagc accgcgtcag cgaggatgcc
    11341 catcaagccg acatgcccgc cgccataaac gagcgtgatc ccttccttcg ccagcagcgc
    11401 gccgagctcg ctcgccgctt gagcaaagcg cggatcgttg cccgcactcg atccgcagaa
    11461 aacacaaagg ctccgcatca cagcgcgcga aaccccgtct cgtcaaccgg cagcggctcg
    11521 cggttccgat cgagccgcgt cacgatccac tgctcgccgc gccgctcgat ctctgtcacg
    11581 atcgacgccc acatcccatc ggccggattc tcgctccagc gaaagcgcat gccggcctcc
    11641 ggcctgacac cgcgctcgcg aagctgcgcc tccgccatgt cgatcagttc cgaagggccg
    11701 atgtggtcgc agggcatgat cgtattgtac ggggacgggg ggtcgggggt cgggggtcgg
    11761 agacaaggct gagcgctacg ccgatgcgcg ccgtcgattt ctccgagatg cgcctcgcgg
    11821 aatcaacagc gaggaacatc tccgacaccc gactcccgac tcccgacccc cgcaacagga
    11881 attcattgaa acttggcacg ttaacgccca aacatgccgc agtttcgata tgacatccgc
    11941 aacgtggcga tcatcgccca cgtcgatcat gggaagacca ctctcgtcga cgctctcctc
    12001 cgccaggccg gagccgtgcg cctcaaccaa cagatggatg agcgcgtcat ggactccaac
    12061 gatctcgaga aagagcgtgg aatcaccatc ctcgccaaga acacctcgat ccgctatctc
    12121 gtcgacgagc ccgacgccct cggccacctc gtgcaccacg aagcgaccca caccgcgtcc
    12181 ggccacgacc ttcccgccgc gttcatccat ccgagcgagg tgaagatcaa catcgtcgac
    12241 acgcccggtc acgccgattt cggcggtgaa gtcgagcgcg ttctgtcgat ggtcgacggc
    12301 gtcatcctcc tcgtcgatgc cgccgaaggc ccgatgccgc agacccgttt cgtcatgcgc
    12361 aaagccctcg gtctggggct tctcccgatc gtcgtcatca acaagatcga tcgtcaggac
    12421 gcgcgtccga cggaagtcgt caacgaggtt ttcgatctga tgatcgagct cggcgcctcg
    12481 gatgagcaac tcgacttccc gatcctctac gccatcggcc gccagggcat cgtccgcacg
    12541 aacctcgaag atacgaccga ggattgcagg ccgttgttcg atgcgatttt gaagcgcatt
    12601 ccgccgccgc cgggttatgc cgatgcgccg ctgcagctcc tcgtctccgc catcgattac
    12661 aacgaatacg tcgggcgcct cggcgtcggc cgcattcagc gcggcaagct gcgccagggc
    12721 gaagacgtcg tcctcatcct tcgcgacggc acacacaaga aaggtcgcat caccaagctc
    12781 accgcgttcg aaggccttcg ccgcgtcgag gtcgaggaag catccgccgg cgagatcgtg
    12841 gccgtagctg gcttcaccga cgtcgagatc ggcgagacat tctcgtccgc tgcgaacccc
    12901 gaggcgctgg aaccgatcgc gatcgacgag ccgaccgtgt cgatgttctg gctggtcaac
    12961 gactcgccgt tcgccggaac cgaagggaag tacgtcacc
//
LOCUS       DATLVW010000594         9062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_603908, whole genome shotgun sequence.
ACCESSION   DATLVW010000594 DATLVW010000000
VERSION     DATLVW010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603908"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /locus_tag="VNN25_17505"
     CDS             complement(<1..2070)
                     /locus_tag="VNN25_17505"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXH93384.1"
                     /translation="MKILTRYIFKEMLGPTALGFSFYTFIILMKNLFDFAGMIIKRSL
                     PASTVARLLYLSLPHIVVLTVPMSLLFGILIAIGRLSSDSEIIAMRALGISTRTIYRP
                     VFLFSVLVFFLNLYLMNFVLPSGNSELQALRSDIFTSQIEKELKPRVFYDEYENLTIY
                     VNDVDPKTLQWKGVFVADSRTAEETRTGTSSGPVTIAQQIQAAHKEAAAPQTILPQRS
                     SNKIIVAESGNLSVMKPSNQVWLNLKTAESHLWDPKKPDRYDLNSNAVQRMRLPDKYS
                     DANGGYQRSLREMTFRELMEQARWAQYSRDADARETYLAAKVEMNKMFAIPFACIVFG
                     ILGLPLGITNRRGGRSSGFSLSIAIILIYYVMINNGEHLADTGKVGPALAMWTPNFIL
                     LALGIYLLIRANRDSGARRSDVGIIKRIVQAIKSRKSKGPVVAGDELAILSRFDITFP
                     NTLDRYIIREFLKILGLVLVSTAALFIIFDYTDLSGDIRANHIPFQTVLSYYRFGVFG
                     ILNYTIPISVLVATLVTFGLFSKNNEVTAFKSSGMSLYRAALPIVGIAIAISFLVYLQ
                     LDYVLPYSNQRVEELRNKIKAKRTAVTAVSTIHQQKLWFLGKGRYLINFLAYDRNEKE
                     LSQVQVFEFHPTKFRLTRRVYAEHARWDGVGWVFSNGWMRSFGDNGTSTYAPITRPLR
                     LYYSERPE"
     gene            complement(2297..3268)
                     /locus_tag="VNN25_17510"
     CDS             complement(2297..3268)
                     /locus_tag="VNN25_17510"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXH93385.1"
                     /translation="MRRVNVGLLGCGTVGRGFVELLNRERERIRSRHGVDVAIRRILV
                     RDAGKQRTGINPALVTTSAIDVLDDGCDIIVELVGGVHAAGAFVRRALRNGHDVVTAN
                     KALLADAGRELFATAQTHDVRIGFEASVCGGIPIIGALQRGLAGDRIDSITGILNGTC
                     NYILTRMEQDDLELADALQQAQARGFAEADPSLDISGVDAAQKLRILSELAFGPMTMR
                     LRVYGIGDVTRSEIEQARSRRCVVRHVAAARRVAGGIELRVERRELPESHPLAAVRDE
                     TNAVVVRGRAVGEIMFQGKGAGSLPTAAAVLADVVGLAQRNYNRVLT"
     gene            complement(3256..4383)
                     /locus_tag="VNN25_17515"
     CDS             complement(3256..4383)
                     /locus_tag="VNN25_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXH93386.1"
                     /translation="MYRFDTLAIHAGQPNDEATGAVTVPIYQTSTFGQLEPGVHRGFG
                     YARTEHPTRRALEECLASLEGARYGLAFASGMAAIHNVLSLLRSGDHVVSTQDLYGGA
                     WRIFTKYFAKFGIDFTFVDTSDLAAVEAAIRPETRLLWLETPSNPLLKVTDVAACSAI
                     ARRAGIRTVVDNTFATPVLQQPIALGADIVLHSTTKYINGHSDALGGAVITDDEALFN
                     DLKFFQNAIGAVPGPQDCFLILRGVKTLGLRMARHCQNAAGVVEYLRAQDQIARIHYP
                     GFGAIVSFELDADVAETIAFTKRLRLWTLAESLGSVKSLFCHPPTMTHASVEPEVRRK
                     VGIADGLIRLSIGLEDVRDLIDDLDQALAEVTVPRRVACGA"
     gene            complement(4659..5456)
                     /locus_tag="VNN25_17520"
     CDS             complement(4659..5456)
                     /locus_tag="VNN25_17520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH93387.1"
                     /translation="MIVGDAALARRLEAFQARVGEANAFARAGGEVLDVAGGKVVFFT
                     PDSPITQAIGVGIGEPVTAEDVDRIEDFFRSRGAACSIHITPWSDPALLAELSSRRYQ
                     FFEFENVFVQRIDAIASNPTDPAIEVHLIEREESATWARVCAAGFATEGIDEETLREI
                     GEAFTAAEGGRCYLASIDGIQCGAAASVSARDLGVVGLFGAATLPEYRGRGVQNALVA
                     RRLAEASGEGCELAFVSTLPGSASHRNVLRRGFELVYTKVSMKRVFD"
     gene            complement(5453..6277)
                     /locus_tag="VNN25_17525"
     CDS             complement(5453..6277)
                     /locus_tag="VNN25_17525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXH93388.1"
                     /translation="MKRGLPERSSMRHDAHFVEELTRRSGRHIGSMIPLELIEPNAEQ
                     PRTQLGNIEELAASIKEKGVLEPILVRNAGPNRYQIISGERRFRAASLAGLDEIPAIE
                     LDVDDKEQLEIALIENIQRKDLTAFEEAEGFLALQQKFGYTHEKISHVIGKSRTTITE
                     TLSINEIPDRIRAMCREAGISNKSILVQIARAGDEEAMEDVVRRFGAGELTRDDVRKK
                     TAKPEPKKAGRPKNFTWQVKDKSLPFSVNLSFRKANVDKSEIAEALRELLRRIESE"
     gene            complement(6305..7060)
                     /locus_tag="VNN25_17530"
     CDS             complement(6305..7060)
                     /locus_tag="VNN25_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXH93389.1"
                     /translation="MIITVANQKGGVGKTTTTINLAAAVANKGFKTLLIDLDPQANST
                     MSYVDPRSISRSMFDVLVSEELGLNDVIVPTPLPNLFLAPSKIALAKLESKLIGELDG
                     PFRLKDKMVAASTEYDYIFIDTPPTLGMITVNAMVASSHVLVPIQSSYFALEGTDDLL
                     ETIEKIKARPNPNLQLLGVVITMHDKRTTLARDIHEQIGQVFGERLFNTVITKSIRLE
                     ESPAYKESIFTFAPRSSGAMEYYSLSEEILSRV"
     gene            complement(7211..7711)
                     /locus_tag="VNN25_17535"
     CDS             complement(7211..7711)
                     /locus_tag="VNN25_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HXH93390.1"
                     /translation="MSELVPMSIKGLMLDPVSNSPIVVLKDEEEKFFLPIWVGIFEAN
                     AIALQLENVETPRPMTHDLLRTAIGELGARVTRIVINDLRDSTFFAQIRVIVNRSGED
                     RTLELDARPSDAIALALRTEAPIYVAQSVLDQAQTITPDDEDQEEQSKTWFEQLDSEE
                     LGKYKM"
     gene            complement(7708..9009)
                     /gene="miaB"
                     /locus_tag="VNN25_17540"
     CDS             complement(7708..9009)
                     /gene="miaB"
                     /locus_tag="VNN25_17540"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXH93391.1"
                     /translation="MPKSFFIETWGCQMNDLDSQRLSGSLKLRGYQRVENESAADLIL
                     LNTCSIRDKSEQKVFSHLGRLREMKAAREDLRIGVCGCVAQQEGEAILHRAPWVDFVM
                     GPGNVGHIDQILAGERRVAIDFPEDRNYDYLTVDRPSSTKAQVTIIEGCNKNCTFCIV
                     PTTRGREVSRPFDDVLREVRAAVDSGRVEIELLGQTVNAYRCPISGRDFGALLGAVAE
                     VDRVARLRFMTSHPAEVSDSMIEAMRDHENISRYLHLPVQSGSSRVLRRMKRLYTREH
                     YLDTIARIRAAIPEIQFSTDVIVGFPGETEEDFEQTISLMTEVGYGSLFAFKYSPRPG
                     TPALKLGAPVDDAVASERLNRLFDLHEQFKREHLGAYRGRILPVLVEGLSKHDPSMLT
                     GRTDDNVVVNFNGDPSTRPGSMLGVRIEQAQHHTLRGEAVA"
BASE COUNT         1733 a         2705 c         2846 g         1778 t
ORIGIN      
        1 ctcgggacgc tcgctgtagt agagccggag cggcctcgtg atcggcgcat acgtagacgt
       61 cccgttatcg ccgaacgagc gcatccagcc atttgagaac acccagccga cgccgtccca
      121 tcgcgcgtgc tcggcgtaga cgcgccgcgt caggcgaaac ttggtcgggt ggaattcaaa
      181 gacctgaacc tgcgagagct ctttctcgtt gcggtcgtaa gcgaggaaat tgatgaggta
      241 acgccccttg ccgagaaacc agagcttctg ctggtgaatc gtcgagactg cggtgacggc
      301 ggtccgcttc gccttgatct tgttccgcag ctcctcgacg cgctgattcg agtaaggaag
      361 aacgtagtcg agctggaggt agaccaagaa actgatggcg atggcgatcc cgacgatcgg
      421 cagcgcggcg cgatagagcg acatcccgct cgatttgaat gcggtgacct cgttgttttt
      481 cgagaagagc ccgaaggtga cgagcgtggc cacgaggacg gagatcggaa tcgtgtagtt
      541 gaggattccg aagacaccga accggtagta cgaaaggacg gtctgaaacg ggatgtgatt
      601 cgcgcggatg tcgccgctca gatcggtgta gtcgaagatg atgaagagcg cggccgtcga
      661 gaccagtacc agccccagga tcttgaggaa ctcgcggatg atgtaacgat cgagcgtgtt
      721 gggaaacgtg atgtcgaacc ggctgagaat cgcaagctcg tcgccggcga cgaccggtcc
      781 cttgctcttt cgcgacttga ttgcctgcac gatccgtttg atgatcccga cgtccgatcg
      841 gcgcgcgccg ctgtcgcggt tggctcgaat gagcaggtag atgccgagcg ccaggagaat
      901 gaagttcggc gtccacatgg ccagcgccgg accgaccttg cccgtatcgg cgagatgctc
      961 accgttgttg atcatcacgt agtagatgag gatgatcgcg atacttaaag agaatcctga
     1021 gcttctgcct ccgcggcggt tcgtgattcc gagcgggagt ccgaggatgc cgaagacgat
     1081 gcaggcgaac ggaatcgcga acatcttgtt catctcgacc ttcgccgcca ggtaggtctc
     1141 gcgggcatcc gcgtcgcgcg agtactgagc ccatctcgct tgctccatca gctcgcggaa
     1201 ggtcatctca cgcagagatc gctggtagcc gccgttcgcg tcggagtact tatcgggcag
     1261 ccgcatccgc tggacggcgt tgctgttgag atcgtatcgg tcgggtttct tcggatccca
     1321 caagtggctc tccgccgtct tgaggttgag ccagacctga ttgctcggct tcatcaccga
     1381 gaggttgccg ctctcagcga cgatgatctt gttgctggag cgctgcggca gaatcgtctg
     1441 tggcgcggcc gcctccttgt gcgcggcctg gatctgctgg gcgatggtga ccgggccgct
     1501 gctcgtgccg gtgcgcgtct cctcagccgt acggctgtca gccacgaaga cgcctttcca
     1561 ctgcagcgtc ttcgggtcga catcgttcac gtaaatcgtg aggttttcgt actcgtcata
     1621 gaaaacgcga ggtttcagct ccttttcgat ctgcgaggtg aagatgtccg atcggagcgc
     1681 ctgcagctcg gagttgccgc tcggcagaac gaagttcatc aggtagagat tgaggaagaa
     1741 aaccaggacg ctgaagagga agaccggccg gtagatggtg cgggtcgaga tgccgagggc
     1801 gcgcatggcg atgatctcgg agtcggatga gaggcgtccg atggcaatca ggattccgaa
     1861 cagcagcgac atcggcaccg tcagcacgac gatgtgaggg agcgaaaggt aaagcagacg
     1921 cgccacggtc gacgccggga gcgaccgctt gatgatcatg cccgcgaagt cgaagaggtt
     1981 cttcatcagg atgatgaagg tatagaagga gaagccgagg gcggtcggcc cgagcatctc
     2041 cttgaagatg tagcgcgtga ggatcttcat cagcggtcag agtttatcgc gagcgtgtga
     2101 atcagagact agagatcaga ggatcagaga tcagagacta gatcagagac atcagagacc
     2161 agagatcaga gacaagtgcc cagagaccgg agatccttga tcagtggaga ccagagatcc
     2221 gagattcgct tctgatctct aatcgctggt cccttgtctc tgcttttcag ctttccacag
     2281 cgtctagtaa gatagattat gttaatacgc ggttatagtt ccgctgcgcg agaccgacca
     2341 catcggcaag cacggcggcg gccgtaggca acgatccggc tcctttcccc tgaaacatga
     2401 tctccccgac agccctccca cgcacgacga ccgcgttggt ctcgtcgcgc accgcggcca
     2461 gcggatgact ctccggcagc tcgcggcgct caacgcgcaa ctcgattccg cccgccacac
     2521 gccgcgctgc cgccacgtgt cgcacgacgc agcgccggct gcgcgcctgt tcgatctccg
     2581 agcgcgtgac gtcgccgatt ccgtatacgc gcagcctcat cgtcatcggt ccgaacgcca
     2641 gctcgctcag gatgcgcagt ttctgcgcgg catcgacacc gctgatgtcg agcgacggat
     2701 cagcttccgc aaacccgcga gcctgagcct gctgcagcgc atccgcgagc tcgaggtcat
     2761 cctgctccat cctggtcaga atgtagttgc aggttccgtt gaggattccg gtgatcgaat
     2821 cgatccgatc accggccagg ccgcgctgca acgctccgat gattgggata ccgccgcaga
     2881 cgctggcctc gaatccgata cgtacgtcat gggtctgtgc cgttgcgaac aattcgcggc
     2941 cggcgtccgc aagaagcgcc ttgttcgcgg tgacgacgtc atgtccattc cgcagcgcac
     3001 gccggacaaa cgcgccggcc gcatgcacgc cgccgacgag ctcgacgatg atgtcgcaac
     3061 catcgtcgag aacgtcgatg gcagaagtcg tgactaatgc gggattgatt ccggttcgct
     3121 gcttgccagc gtcgcgcacc aggatccgtc ggatagcgac gtcgacgccg tggcgcgaac
     3181 gaatacgctc gcgctcacgg ttgagaagct cgacgaagcc gcgcccgacg gtgccgcaac
     3241 cgagcagccc gacgttcacg cgccgcatgc caccctcctc ggcacagtca cttccgccag
     3301 cgcctgatcg agatcatcga tcaggtcgcg cacgtcttcg agaccgatcg acagccgaat
     3361 gagtccgtcc gcgatcccga ccttccgcct cacctcgggt tcgacggacg cgtgtgtcat
     3421 cgtcggcgga tgacagaaaa ggctcttcac gctgccgaga ctctcggcga gtgtccagag
     3481 ccggaggcgc ttcgtgaatg cgatggtctc ggcgacatcc gcgtcgagct cgaaggaaac
     3541 gatggcgcca aagcctggat agtgaatgcg cgcgatctgg tcctgcgcac gcagatactc
     3601 gacaactccc gcggcgttct gacaatgacg ggccatccgc agtcccagcg tcttcacgcc
     3661 gcgcagaatg aggaaacaat cctgagggcc cggcaccgcg ccgatcgcgt tctggaagaa
     3721 cttcaggtca ttgaaaagcg cctcatcgtc ggtgatcact gccccgccga gcgcgtcgct
     3781 gtgaccgttg atgtacttcg tcgtcgagtg gagaacgatg tccgcgccga gcgcgatcgg
     3841 ctgctgcagc accggagtgg caaaggtgtt gtcgacgacg gtgcggattc cggcgcggcg
     3901 cgcaatggcg ctgcatgccg caacgtctgt gaccttcagc aacggattcg acggcgtctc
     3961 cagccagagg agtctcgtct cgggccgtat cgcggcttcg accgcggcga ggtcgctggt
     4021 gtcgacgaac gtgaagtcga tcccgaactt tgcgaagtat ttcgtgaaga tgcgccaggc
     4081 gccgccgtac agatcctgcg tcgagacgac atggtcgccg cttcgcagca gcgacaggac
     4141 gttgtgaatt gccgccatgc cgctggcaaa cgcgagcccg tatcgcgcgc cctcgagcga
     4201 ggcaagacac tcttcgagcg cgcgccgtgt cggatgctca gtgcgcgcat agccgaagcc
     4261 gcggtgaacg ccgggctcga gctggccaaa ggttgaggtc tggtagatcg gaacagtgac
     4321 ggcgccggtg gcctcgtcat tgggctgacc ggcgtggatt gcaagcgtat cgaagcggta
     4381 catcgttccc ctcctgccgc acgaatggcg gcggatgttc gctgctgact tgtcggggag
     4441 aaaagctgta ggagggaccc gggtcgggga agcagtcagt ctacgcgtcc gcgcccgggc
     4501 ggcgcatcac cattcggcgc atcgacatca tggtgtggcg agacatgttg gggcgggagg
     4561 ataaaggtac gagctggccg tgtcaactgt tcacgcgaag acgcgaagac gcgaagaaaa
     4621 ggaattcaaa gtcaattcga gtcttcgcgt gaacgtttct agtcaaacac cctcttcatc
     4681 gacaccttcg tgtaaaccag ctcaaaaccg cgccggagaa cattgcgatg ggacgcactt
     4741 ccgggcagcg tcgagacgaa tgccagctcg catccctccc cgcttgcttc tgccagcctg
     4801 cgcgcgacga gcgcattctg cacccctcgg ccgcgatact cgggtagcgt agcagcaccg
     4861 aacagtccga cgacgccgag gtcgcgggcg cttaccgatg cagccgcgcc gcactgtatt
     4921 ccatcgatcg atgcgagata gcaacgaccg ccttccgcgg ccgtaaacgc ttctccaatc
     4981 tcgcgaagcg tctcttcatc gattccctcg gtggcaaatc cagccgcaca gacgcgagcc
     5041 caggtggcag attcctcgcg ctcgatgagg tggacttcga tcgccggatc ggtgggattc
     5101 gaggcaatcg catcgattct ctgcacgaag acattctcga actcgaagaa ctggtaacga
     5161 cgcgacgaca actccgccag caaagccgga tcgctccacg gcgtgatgtg aatcgagcag
     5221 gcggcgccgc gcgagcgaaa gaaatcctcg atgcggtcga cgtcctcggc cgtcaccggt
     5281 tcgccgatac ccacaccgat tgcctgcgtg atcggtgagt ctggcgtaaa gaagaccact
     5341 ttgccgccgg cgacatcgag aacttcgcct ccggctcttg cgaacgcgtt cgcttcgccg
     5401 acccgtgcct gaaacgcttc gagccgccgt gccagcgccg catcgccgac gatcattcgc
     5461 tttcgatccg gcgcagcagc tcgcgaagtg cttccgcgat ctcgctctta tccacgttcg
     5521 ccttgcggaa gctgaggttg acgctgaacg ggagcgactt gtccttcacc tgccaggtga
     5581 agtttttcgg gcggcccgct ttcttcggct cgggcttcgc cgtctttttg cggacatcat
     5641 cgcgcgtcag ctcgccggcg ccgaaacggc ggacgacatc ctccatcgcc tcctcgtcgc
     5701 cggcgcgggc gatctgcacg aggatggact tgttggagat gccggcttcg cgacacatgg
     5761 cgcggatgcg gtcggggatc tcgttgatcg acagcgtttc ggtgatggtc gtgcgcgact
     5821 tgccgatgac gtgcgagatc ttctcgtgcg tgtagccgaa cttctgttgt agcgccagaa
     5881 agccttccgc ctcttcgaac gcagtcaggt ccttgcgctg gatgttttcg atgagcgcga
     5941 tctcgagttg ctctttgtcg tcgacgtcga gctcgatcgc tggaatctcg tcgagtccgg
     6001 ccagcgaggc agcgcggaaa cggcgctcgc ctgagatgat ctggtagcga ttggggccgg
     6061 cgttgcggac gagaatcggt tcgagcacgc ctttttcttt aatcgacgcc gccagctctt
     6121 cgatgttgcc gagttgcgtg cgcggctgtt cggcattcgg ctcgatgagc tcgagcggaa
     6181 tcatcgatcc gatgtgacgc ccggagcggc gcgtcagctc ttcgacgaaa tgcgcgtcgt
     6241 ggcgcatgga ggaacgctcg ggtaggccgc gtttcatgga catacggttt cctcgggggt
     6301 tgcctcagac acgggacagg atctcctcac tcagcgagta gtactccatc gctccgctcg
     6361 atcgcggcgc aaacgtaaag atcgattcct tgtaagccgg actctcttcg agccggatcg
     6421 acttcgtgat cacggtgttg aaaagccgct cgccgaagac ctggccgatc tgctcgtgga
     6481 tgtcgcgcgc cagcgtggtg cgcttatcgt gcatcgtgat cacgacgccg aggagctgga
     6541 ggttcgggtt cgggcgggct ttgatcttct cgatcgtctc gagcagatcg tcagtgcctt
     6601 cgagcgcgaa gtacgacgac tggatcggca ccagcacgtg gctcgacgcc accatcgcgt
     6661 tgacagtgat catgcccagc gtcggcggcg tgtcgatgaa gatgtagtcg tactcggtcg
     6721 acgcggccac catcttgtct ttgagccgga acggtccgtc cagctcgccg atcagtttcg
     6781 attcgagttt ggccagtgcg atcttcgacg gcgcgagaaa gagattcggc agcggcgtcg
     6841 ggacgatgac gtcgttcagg ccgagctctt cgctgacgag cacgtcgaac atcgaccgcg
     6901 aaatgctccg cggatcgacg tagctcatgg tgctgttggc ctgcgggtcg aggtcgatca
     6961 gcagtgtctt gaagcctttg ttggcgacgg cggcggccag attgatggtg gtggtcgttt
     7021 tgccgacccc gcctttttgg ttggcgacag tgatgatcat tgaacgggct cgattgtaac
     7081 ggacaacggc gcgcgggagt cggttgtcgg gagtcgggag tcggagaaat ctggcgcgat
     7141 tagtcggcta tcggaggtaa ggacgttgac tttctccgac ccccgacaac cgacaaccga
     7201 ctcccccacc tcacatcttg tacttgccca gctcctcgct gtccaactgc tcgaaccacg
     7261 ttttcgactg ctcctcctga tcctcgtcgt ccggcgtgat ggtctgcgcc tgatcgagaa
     7321 cggattgggc aacgtagatc ggcgcttcgg tacgcagcgc gagagcgatg gcgtcgctcg
     7381 ggcgggcgtc gagctccagg gtgcggtctt cgccggagcg gttcacgatc acccggatct
     7441 gtgcgaagaa tgtcgaatcg cgaagatcgt tgatgacgat gcgcgttacg cgggcgccga
     7501 gctcaccgat ggcggtgcgc aggagatcgt gggtcatcgg acgcggcgtc tccacgttct
     7561 cgagctgcag agcgatggcg ttggcctcga agatgccgac ccagatcggg agaaagaact
     7621 tctcttcctc atctttcaaa acgacgatcg gcgagttgga tacggggtcc agcatcagac
     7681 ccttgatcga catcggtacg agctcactca tgcgacggcc tccccacgta acgtgtgatg
     7741 ctgcgcttgc tcgatgcgca cacccagcat actccccgga cgcgtcgatg ggtcaccgtt
     7801 gaaattgaca acaacgttgt cgtcggtacg tcccgtgagc atggaaggat cgtgcttact
     7861 caaaccttcg acgagcacgg gcaggatgcg gccgcgatac gcgccgaggt gttcgcgttt
     7921 gaactgctcg tgaaggtcaa aaagacggtt gaggcgctcc gaggcgaccg cgtcgtcgac
     7981 cggcgctccg agcttcagcg ccggcgtgcc ggggcgcggc gagtacttga aggcgaacaa
     8041 agatccgtag ccgacttcgg tcatcagcga aattgtctgc tcgaaatcct cctcggtctc
     8101 gccggggaac ccgacgatga catcggtcga aaactggatt tccgggatgg cagcgcggat
     8161 gcgcgcgatc gtgtcgagat agtgctcgcg cgtgtagagg cgcttcatcc ttcgcaggac
     8221 tctcgatgat cccgattgca ccggcaggtg gagatagcgc gagatgttct cgtggtcgcg
     8281 cattgcctcg atcatcgagt cgctgacttc agccggatgc gaggtcatga agcgcaaccg
     8341 cgccacgcga tcaacctccg cgaccgcacc gagcagcgcg ccgaagtcgc gtccgctgat
     8401 tgggcatcga tacgcattga cggtctggcc gagcagctcg atctcgacgc ggccgctatc
     8461 cactgcggcg cgaacttcgc gaagcacatc gtcgaacggg cgggagactt cgcggccgcg
     8521 cgtcgtcggc acgatgcaga aggtgcaatt tttgttgcac ccttcgatga tcgtgacctg
     8581 ggctttggtg gacgagggac gatcgacggt caggtagtcg taattgcgat cctcggggaa
     8641 gtcgatggcc acacggcgct cgccggccag gatctggtcg atgtggccga cattacccgg
     8701 ccccatcaca aagtctaccc acggtgcgcg atgcaggatg gcctcccctt cctgctgcgc
     8761 tacacagccg cagacaccga tgcggagatc ctcacgcgcc gctttcatct cgcgaagtct
     8821 gccgagatgc gaaaacacct tctgctcgga cttatcgcga atcgaacagg tattgagcag
     8881 gatgagatcc gccgcgctct cattctccac gcgctgatag ccgcggagct tgaggctgcc
     8941 ggagagccgc tggctgtcga gatcgttcat ctgacagccc cacgtttcga tgaagaagga
     9001 tttgggcatg gactgctcag caacggttac ttcaacgccg ccaacgcatc ccgcgcctca
     9061 gc
//
LOCUS       DATLVW010000595        19883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_359892, whole genome shotgun sequence.
ACCESSION   DATLVW010000595 DATLVW010000000
VERSION     DATLVW010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359892"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..641
                     /locus_tag="VNN25_17545"
     CDS             183..641
                     /locus_tag="VNN25_17545"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXH93392.1"
                     /translation="MKPLETTVEEVLRKAIESEIESRVYYQKLAERAASPAVRARMLQ
                     LADFELVHRAKMERRYREEIGHPPPPPAPATVELPSDLVNLDMSHALKHALERERDSE
                     SYFRFMAERAPDTILGALFMELAEIEWKHKTDLQAEYDAAAPPDSFLNDL"
     gene            801..2249
                     /locus_tag="VNN25_17550"
     CDS             801..2249
                     /locus_tag="VNN25_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93393.1"
                     /translation="MPRFALCLFLTFSLPLLAAGHDLTKVPPAANQIYPVVTGNGSGF
                     TTAWMESAISRNAVVSSVVSAGGEPIEGAGAAIDQQPVRSVAIAHSPTETLVVWVAGQ
                     NVFAERLSLSGMPLNTIVLTSDKTYPSSVAVAWNGSRYFVVWSNQFQLLGAFIAPDGS
                     ATPPHALFSEPSIDAQSAPTELPVMPDVAWDGRNFIVVFADLPKLICYTLCPSPNPDQ
                     FRVMRVSANGDAIDSAPLVIAGAHVMAHVASSGTESLITLDSFGQVSAIIAHDEGGLT
                     LEAETPLFRWFSDVSSAVVWDGSAYTVGWRYVGHTPGPSWMGVAHVTPSGLRFAYRFT
                     AAGSAFPYGGTTSWGRPSAAVNETGITAFTISEATAPSSFVRARLYLASELAPMPAPP
                     PAPGNVVNYVSGNTARIDWQSETADGFAVEWSWDSGKSWSVYATVSGDARTTTVYARP
                     GIQFRVRALGPGGMSEGTVTSIASPQRRRASH"
     gene            2284..3807
                     /locus_tag="VNN25_17555"
     CDS             2284..3807
                     /locus_tag="VNN25_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93394.1"
                     /translation="MTRGILSTGVFPAAASRLLNPMMRTSVLILTTLFSSSLLAAGHD
                     LTRLAPAVNQLSPVVTGNGAGFTAAWMEPAQSRNTIVSQVANAGGEPIAGMGAAIDQT
                     NVQSVAIAHSPSETLVVWVANGNVFAERLSPSGLPLGTTAVTSVQDFAQDVSVAWNGS
                     RYFVVWSNAFQLLGTFIASDGSSTSPRPLFGEPVVIGQRAPANFELVPDVAWDGRNFI
                     IVFGEAPDLPCNISACPSPPANPLHFRVMRVSADGDAIDSAPLTISGIRLRAHVASSG
                     AESLIALDIYGQVSTITVHDEGGLSLDAETPLFRWFSSVSSTVAWDGATYTVGWRYVG
                     VNPTPSWLGAAHVTRSGSPLDYRFVASDGALPFSGTAAWGLPSIAVNEAGITAFAMSE
                     IAAPSSLARARLYLASELAPMPAPPPAPRNVVSYFSGNMARIDWQSETAAGFAIEWSG
                     DFGTTWRSFTYISGDARTVNVAAHVGNLFRVRAIGPGGLSEGTITSIGSQQRRRASY"
     gene            complement(4020..4436)
                     /locus_tag="VNN25_17560"
     CDS             complement(4020..4436)
                     /locus_tag="VNN25_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93395.1"
                     /translation="MIDLRSRALIVEDDAAARGLLGQLLESLDFAFDLAADGEEALRF
                     LGDQEYDVILLDIVLPKVSGIEVLESLHRHRPRLLGKVIVVTGLDVREIRSLFPAVHE
                     TLSKPVLPGRLREAVRSCVTRRGQGATIVDERITPD"
     gene            complement(4429..5055)
                     /locus_tag="VNN25_17565"
     CDS             complement(4429..5055)
                     /locus_tag="VNN25_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXH93396.1"
                     /translation="MFLFDGPEDAAITYAFAHGAGGAMDTPFMTTVARALGARGIRVV
                     RFEFPYMAARRTGGKRGAPDRQPVLLATWRSVVETLENRERLFIGGKSMGGRMATMVA
                     DELGVRGLVCFGYPFHPPGQPAKVRTAHLETLSTPMLVLQGERDPFGNREDVATYLLS
                     PSIEVEWLRDGDHSLKPRASAGVTEKENLALAVANAEAFMARIALLND"
     gene            5080..5388
                     /locus_tag="VNN25_17570"
     CDS             5080..5388
                     /locus_tag="VNN25_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93397.1"
                     /translation="MRFRLFAFALSVASIASIASAATPPPNTIAPPTIAHPMAMFLGE
                     LSHDGKQKVTFRASALGTHFFLEEAAGVTVYVYDETVGYRKETFLKGSTLAKAVKKYR
                     "
     gene            complement(6310..7515)
                     /locus_tag="VNN25_17575"
     CDS             complement(6310..7515)
                     /locus_tag="VNN25_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXH93398.1"
                     /translation="MLKDLDLLMRERELDALLVPMHEAMHPSFRWITRGAKVTRGYAV
                     KLAGRDPILISYPMERDEASATGLTTRLIHDFGYDAIFKSAANAAEAYGLFFDAVLRE
                     LGAKSTVAIVGNLPFPLYLGIAEGLGARGWSVWRGSDDLVQLARKRKEPWEVEAIRSA
                     GERTEQVVDCVREVLRNATISDRQLVHDGRPLTLGDLKRLVSHEIAARGMVEDHDTIL
                     SQGRDAGIPHSRGDAEAVVRPSVPIVLDIFPADRATGYFFDLTRTFCVGPIPDELHRI
                     HADVLAAFELARAEMHAGTFASSYQALVCDFFEKRGYATTRSNPATLEGYVHSLGHGV
                     GLDVHEKPSFSLSPSNGDEIERGDIVTIEPGLYFPDREIGVRIEDTLVVGDDGRVETL
                     CRSDRGLVP"
     gene            7608..8258
                     /locus_tag="VNN25_17580"
     CDS             7608..8258
                     /locus_tag="VNN25_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93399.1"
                     /translation="MGALILFNASYRFAVFGNAWATTASLASYNFSGVTMSKRRRARQ
                     AQKKLDKKRARARRAPVLVAAPVVPCIAPDPLEAAAAILGRVAEDLMNAHQILDDVSN
                     IGVWSKSDDCSDNRQRRKGLREAGSGVSTARRRLTDLETTEPELQRDFALLDADIRAV
                     VADLEPLPDTVSDIMDRNPALDVSIYDAMARIDALRERSLGISSEYTRRAQLARQQ"
     gene            complement(8287..8682)
                     /locus_tag="VNN25_17585"
     CDS             complement(8287..8682)
                     /locus_tag="VNN25_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH93400.1"
                     /translation="MKQTIGLVSLVVREYDEALAFYVGVLGFELIEDSFVPAQNKRWV
                     VVAPPGSTTESRLLLARAANEEQASRIGNQTGGRVFLFLYTDDFARDYQNYRDKGVTF
                     VREPKEEPYGTVAVFQDLYGNLWDLVELR"
     gene            complement(8776..9105)
                     /gene="trxA"
                     /locus_tag="VNN25_17590"
     CDS             complement(8776..9105)
                     /gene="trxA"
                     /locus_tag="VNN25_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXH93401.1"
                     /translation="MATSQAVREISDQDFEELVNGGQPVFVDFWAPWCGPCRIIGPLV
                     EELAPSYDGKVVITKMNVDENQNVPQKFGVTSIPTLMIFKNGKLIDRAIGAMPKPALQ
                     QFIDRNI"
     gene            9364..10077
                     /locus_tag="VNN25_17595"
     CDS             9364..10077
                     /locus_tag="VNN25_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93402.1"
                     /translation="MSDPRPHGHERRSFPRPPLWLNLLLLIIAAGTFVYAKHQRDVIE
                     EKTAILFRHTANSPVELNRMRDELSQMDLTQAQLKHEIDARMQYLQSQQSAQFYISID
                     TQRKKMQFRFGRDIVRETDVQIGEAKTIKSPTGKTWTFLPLKGGFSVAGKEDGASWPV
                     PEWVYAMHGEREPAERRVVPNGLGRYVIFLQDSYVIHSPPPPESPLQGPKPGSFMVPE
                     ADLAAIWPRITNDTRVYIF"
     gene            10077..11015
                     /locus_tag="VNN25_17600"
     CDS             10077..11015
                     /locus_tag="VNN25_17600"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXH93403.1"
                     /translation="MKRWIGFLGSWALLVLAFGLAAFAVAGETINMRDERRLDRVAEI
                     DRLINEDGGSKASVLRNTASQKTANVNALQSKLQDVEKQTDDVPDTGQTILVSTAENK
                     LYVRRGGQTIFEAVCSTGKGTTLAVDGKTILFDTPIGKLHIISKDENPQWVPPDWHYV
                     EEARKNGMRVVHLDPGQTIDARTGQPVSSKPDAGVWSWLSGGNNNSTSNDVLKVRGDT
                     VVEVNNGVERELEPGKTIVAGDAVIVPPVDTKQRHYDKVLGKFRLEMGNGYGIHGTDE
                     PDKLGQSVSHGCVRLGDADIEKLYQIANVHDTVIIY"
     gene            11233..11847
                     /locus_tag="VNN25_17605"
     CDS             11233..11847
                     /locus_tag="VNN25_17605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXH93404.1"
                     /translation="MHPWRERLARWFTPLARRMPLSPNAITLIAFALNLIGAACLAYG
                     GERPPLFLVAIIFIAISGFADALDGIVARVQNKTSRFGDFLDHCADRAGDTLLAACWL
                     YGNNVRQPLAMAGIIMTMLNGYIGTQIEATYGERSYESIGRGEFVLGIIILPIVSYSL
                     FTNGWQALQLARLTVAEWLAVVVLLFALYGVVQRVRVAIRLERS"
     gene            11844..12665
                     /locus_tag="VNN25_17610"
     CDS             11844..12665
                     /locus_tag="VNN25_17610"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HXH93405.1"
                     /translation="MTDELNPITGEPVIVAPQREDRPNVYEGAPCPFCPGAENETPPE
                     ICRDGDPWRIRVFPNRYPPTEHSEIIVESALHDDTFDALAPDHAKRVVELYFERYRAL
                     AAKTAYVCIFKNHGRRAGASMPHLHSQLVGTPFIPARIARESAAFAEAANCPLCDLRE
                     HPLIVESEHYRWIAPRGARLAWQQWIVPKAHENQLDEPHELASLLQSSVRAMSTISDS
                     FNWAFVVFPGEPRAGHWYLELLPRIGAVAGFELGTGTFVNTVSADDVATLFSRFK"
     gene            complement(12676..13392)
                     /locus_tag="VNN25_17615"
     CDS             complement(12676..13392)
                     /locus_tag="VNN25_17615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXH93406.1"
                     /translation="MLPLADHNPRRTTPVVNIILIVINVLMFFWEISLGPQFEPVLTN
                     VAFVPARFWYLPDSLLPNIITMFVSMFLHGGWLHLGGNMLYLWVFGDNVEDRLGHVKY
                     FFFYIACGLIATLTHAAMNPTSRVPSIGASGAIAGVLGAYIVLFPKQRVTTLIPIFIF
                     ITVREIPAVFLLGFWIVVQVFFGAASMNGDLQSAGGVAYFAHIGGFIAGMLLVVLLGG
                     LRRPPLRPVPQDYRWRNGRL"
     gene            13607..14173
                     /locus_tag="VNN25_17620"
     CDS             13607..14173
                     /locus_tag="VNN25_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXH93407.1"
                     /translation="MRLFTSIACVTLLAGCFAISRHTPSLIVAAREGDAPAIRAIVKR
                     GCDPNAAYGALNWTPLMHAISRNQIEAVKALLDSGADVNKTGDYETEPRTKQLRVTPL
                     MMAAGYGYTPIVELLLQRGADPTPADSDGNRALDYALTGTLYRRTLFECQDSTVRALL
                     AAHAPRSVTADSRKWAGIKRCETLALLR"
     gene            complement(14247..15368)
                     /locus_tag="VNN25_17625"
     CDS             complement(14247..15368)
                     /locus_tag="VNN25_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXH93408.1"
                     /translation="MRFETKAVHSGAGADDETGAIAAPIHLSTTFERRAEDGEPSHGF
                     SYIRDGNPTQARLEEALAAIDSGSHALAFASGMAATTCLLQALPAGSHVILQDESYYG
                     VRALANEFLNRWGLTFDFVAMDDLDAVKSAVRPTTRAIWCESPSNPLMRIADVAALAR
                     IAHEGNAILIADATFATPALQRPIEQGADVVLHSTTKYLGGHSDVQGGSLAFAQRGAL
                     TEAVEHCRKITGGVASPFNSWLILRGIRSLPARMRVHCENAMALARALANHPRVEAVH
                     YPGLESHPGHAIAAKQMSAFGGMLSFNVRGGREAALAVTAKTKIFTRATSLGGTESLI
                     EHRNSSEGPGSRTAENLLRISTGLEHADDLVEDLLRALD"
     gene            15416..16303
                     /locus_tag="VNN25_17630"
     CDS             15416..16303
                     /locus_tag="VNN25_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93409.1"
                     /translation="MARRSAFLAVLVFMTAAAALAQPVMTYVYNAPESALDVRYQYHW
                     EILRTALEKTTPKWGPYRMVKSELMTERRQALELKNATGKLTVMYLSTTPEFERSLIP
                     IHIPVDKNLGGYCVFLIRKEEQPRFANVRTLADLHKFTFGLGLGWVDVDILNAAGLKV
                     VTGSSYDGLFEMLARKRFDIFLRAAVEVLDEYDQRKSRLPDLDIERNLILYYPLPMYF
                     WFPKTAEGRRLATRAEEGMRMMIADGTYDRIFDKYQRHKIERLRLKSRQIIKIGNPFL
                     GPEVPFSDKRLWFDPQTYK"
     gene            16303..18456
                     /locus_tag="VNN25_17635"
     CDS             16303..18456
                     /locus_tag="VNN25_17635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93410.1"
                     /translation="MPARRSSLAMVVAATLVAVTTVLLGIYGIISYRLDARAQRKALQ
                     DLTTIQANELSVALALPIWNVDRAQIDKVIEAMTKPKSVSGISVHTAGEDIGRLRKPD
                     GKFRLWDGKSTPADMLVEEREIYFGDKQIGTVRLLVTKEYLNTDLRMLLIRIVGMIVG
                     VDLLVVLGTYFILLRVVVRPLNEIERYAVAVSTGVGPQNAPQPESLFTADLENLRSSI
                     AKMVLQLEQRYTELKEQEDFTNTAINTMTGIFFIQDREGRFIRWNDNMARVADGEQLL
                     SDRVGRPMVHPDDRAMIEEKVAEIFDTGHAEVETRMVLKAGIRNYLFNARRMDVRGQP
                     FMVATGVDITERRQAEAEQRRLQSEIARSAAEWQETFDTVTTPILITEENGAIVRVNR
                     AALELSRLSEEQIKGKPIGQLPSREPWHTAGQLVSYITTEGHGTSAETKDAEGRTWDI
                     VVSHFSAPNDGGERFILVLWEITGIVELQESLRRSETLSAMGNLVAGVAHEVRNPLFG
                     ISATLDAYQDEMSRPGYVEFAATLRQEVNRLIHLMKELLEYGKPGALSIERGHLRDVV
                     GSAVESRQQAARAAHVRIVNQMTNGIPTLLMDSSRLRQVFENLIDNAIQHSERGSKVQ
                     ISSSIVEHAGRHWVECRVDDEGTGFATENLERVFEPFFTMRDGGTGLGLSIVHRIVEE
                     HSGKVFAANRPEGGGSIRVLFPLGGTEAAAKSVAS"
     gene            18462..19847
                     /locus_tag="VNN25_17640"
     CDS             18462..19847
                     /locus_tag="VNN25_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH93411.1"
                     /translation="MTRNRVLIVDDEPSIRMAIRRFLTTSGFEVSDADSRSSALALAR
                     KERPDAIILDYKLRDCTALDLLPQFLAIDAEVAIIVLTAHGSVELAVSAIKEGAQQFL
                     TKPVELPTLLVILNRTLDTQRSRQADQADRKKESRDVLDPFTGTSAAVRQLESDVDKI
                     VSTDRPILIQGETGTGKGILSNWLHRNGRRKQEPFVDLNCAGLSKDFLESELFGHEKG
                     AFTGAVQAKQGLFDLAHRGTLFLDEIGDVDLQVQPKLLKVLEDKRFRRLGDVKDRFAD
                     VRLIAATHHDMNNLVRTRRFREDLYFRISTIVLRLPALRERPEDIEALAADLLARISH
                     ELGRSGTSLAAPAVRALQQYAWPGNIRELKNVLERSLLLSERNEIQRGDLRFEHPTSE
                     GAAEDTRLTLTEVEARHITRVLKEEDGVIDRAAKRLGVTRNTLYYKIRKHRIALRKNV
                     GADASDSESRA"
BASE COUNT         3858 a         6257 c         6100 g         3668 t
ORIGIN      
        1 tcgtcccgcc gaacttccct gtcggaccga tgggatggcg gccgtactac ggctcgatcg
       61 acaaacgggg caccgcgggc ggcaccggcg tcggtcagca actacgcagc gccccgttcg
      121 aggacacggc cccgccgaat cccgatgtgt ttcgcggcgg agaagcgcaa taatcgcctc
      181 gcatgaaacc gctcgaaacg acggttgaag aagtcctgcg caaggcgatc gagtccgaga
      241 tcgagtcgcg cgtctattac cagaagttgg ccgagcgcgc agcctcgcct gccgtacgcg
      301 cgcgcatgct gcagctcgcc gacttcgagc tcgtgcaccg cgccaaaatg gagcggcgct
      361 accgcgaaga aatcggccat ccgcctcccc cgcccgctcc ggcaaccgtg gagttgccgt
      421 ccgatctggt gaatctcgac atgagccacg ccctcaaaca cgccctggag cgcgagcgcg
      481 actcggagag ctacttccgt ttcatggccg agcgcgcgcc ggacaccatc ctcggcgcgc
      541 tgttcatgga actggccgaa atcgaatgga agcacaagac cgatctgcag gcagagtacg
      601 acgccgcggc gccgccggac agcttcctga acgatctctg agccgtcacc gcgaagccga
      661 gaggtttgtg ctatctcagc gtgaatgctg agacgccgaa gcgtaattga agtgggggcg
      721 ttctctccct acaacaccct cgcgaccgct tccacggctg gaataacgga gtagcgtgca
      781 ttattcgcag gaagatacca atgccccggt ttgccctctg ccttttcctg actttctcac
      841 tgccgcttct cgccgccgga cacgatttaa ccaaagtgcc gccggcagcg aatcaaattt
      901 acccggtcgt gaccgggaac ggctccggct tcacgacggc gtggatggag tcggcgatca
      961 gccggaatgc cgtcgtctcg tccgtggtga gcgcgggagg tgagccgatc gagggggcgg
     1021 gcgccgcgat cgatcagcag cccgttcggt cggtcgcgat cgcccacagt ccaaccgaaa
     1081 cgctcgtcgt atgggtcgcc ggccagaacg tattcgcgga acggctgtct ctttcgggca
     1141 tgccgctcaa tacgattgtc ctcacgtctg acaaaacgta tccgtcctct gtcgcggtgg
     1201 cgtggaatgg cagccgctac ttcgtcgtct ggtcgaacca gtttcagctc cttggtgcct
     1261 tcatcgcacc agacggctcg gcgacaccgc ctcatgctct tttcagcgaa ccttccatcg
     1321 atgcgcagag tgccccgacg gagttacctg tgatgccgga tgtagcctgg gatggccgga
     1381 acttcatcgt cgtttttgcg gaccttccga agctcatctg ctacaccctc tgcccctcac
     1441 ccaatccgga ccagtttcgt gtgatgcggg tctccgcaaa tggcgatgcg atcgacagcg
     1501 cgccactcgt catcgcaggc gcgcacgtga tggcacacgt agcctcgagt ggcaccgagt
     1561 ccctgattac actcgacagc ttcggtcagg tctcagcgat catcgcgcac gacgagggcg
     1621 gcttgacgct cgaggcagag actccgctgt tccgatggtt ttcggacgtt tcgagcgcgg
     1681 tggtgtggga tggctctgca tacaccgtag gctggcgata cgtgggccat actcctggtc
     1741 cgagctggat gggtgtagct catgtaacac catcgggtct tcgattcgcg taccggttta
     1801 cggcggccgg cagtgccttt ccctacggcg gcactacatc gtggggacga ccctcggccg
     1861 cggtcaacga aaccggtatt acggcattca ccatttcgga agcgacggcg ccatcttcgt
     1921 tcgtacgggc ccgcctctat ctcgcgtccg agctcgcgcc gatgcctgcc ccaccgcctg
     1981 caccgggaaa cgtcgtcaat tacgtcagcg gcaatacggc gcgtatcgac tggcaaagtg
     2041 aaacggcgga cggattcgca gtggaatggt cgtgggattc cggcaagagc tggagtgtct
     2101 acgcaaccgt ctccggcgac gcccgcacca ccaccgtcta cgcccgaccc ggcatccagt
     2161 ttcgcgtgcg cgccctcggg ccgggtggaa tgtcggaagg aacggtcacg agcatcgcca
     2221 gtccgcagcg ccgccgcgcg tctcactgac gcagccggtg gtttctgcta tacggccatg
     2281 atgatgacac gcgggattct ctcaaccgga gtcttccctg cggcggcgag ccggctattg
     2341 aacccgatga tgcgcacttc cgttttgatt ctcacgacgc tcttctcatc ctcgctcctc
     2401 gcagcgggcc acgatttgac gaggttggcg ccggcagtga atcaactgtc gccggttgtg
     2461 acaggaaacg gagccggctt cacggcggcg tggatggagc cggcgcagag tcgcaacacc
     2521 atcgtgtcgc aggtggcgaa cgccggcggg gagccgatcg cgggcatggg cgctgcgatc
     2581 gaccagacga acgttcagtc agtcgccatc gcccacagtc catccgaaac actcgttgtg
     2641 tgggttgcta acggcaacgt attcgcggaa cggctgtctc cgtctggcct gccgctgggc
     2701 acgaccgccg ttacatccgt tcaggacttc gcccaggacg tgtcggtggc ctggaacggc
     2761 agccgctact tcgtcgtctg gtcgaacgcg tttcagctgc tcggtacctt catcgcatcc
     2821 gacggctcct cgacatcacc tcgcccatta ttcggcgaac ctgtggtgat cggacagcgt
     2881 gctcccgcga atttcgagct cgtgccggat gtggcgtggg acgggcggaa cttcatcatc
     2941 gtgtttggcg aagcaccgga cctcccatgt aacatttccg cctgcccatc cccaccagca
     3001 aacccactcc attttcgcgt aatgcgcgtt tctgcggacg gcgacgcgat cgacagcgcg
     3061 ccgctcacga tcagcggcat tcgccttcgc gcgcacgtgg cctcgagtgg tgcggagtcg
     3121 ttgatcgccc ttgacatcta cggccaggtc tcgaccatca ccgtccacga cgagggcgga
     3181 ctgtctctcg atgcggagac ccctttgttc cgatggttct cgtccgtttc gagcaccgtt
     3241 gcgtgggatg gtgcgacgta caccgtcggc tggagatacg tgggcgtaaa tccaactccg
     3301 agctggctag gcgccgcgca tgtgacacga tcgggctcgc cactcgatta ccgcttcgtg
     3361 gcgagcgacg gcgcactgcc gttcagcggc acggcggcgt ggggactgcc atcgattgca
     3421 gtcaacgaag ccggtatcac ggcattcgcc atgtcagaaa tagcagcgcc gtcgtcgttg
     3481 gcgcgggctc gcctctatct cgcgtccgag cttgcgccga tgcctgcacc tccgcccgca
     3541 ccgaggaacg tagtcagtta cttcagcggc aatatggcgc gtatcgactg gcaaagtgaa
     3601 acggccgccg gattcgctat cgaatggtcg ggggatttcg gcactacttg gcgctcgttt
     3661 acctacatct ccggcgatgc gcgcacggtc aacgtcgccg cgcatgtagg caatctcttt
     3721 cgcgtgcgcg caatcgggcc gggcggtctg tcggaaggaa cgatcacgag catcggcagt
     3781 cagcagcgcc gccgtgcgtc ttactgacag aaaacgccgg caaaaaacca cgggcggttg
     3841 caagcgacgc gcttgcaacc gcccgttttg ctctctctca tacaacacgc ttccggaagg
     3901 aggcgtactg catgtcccag tggaggagag tctaggcagg cgccatcagg caaccgtgcg
     3961 tcgcaacata aaaattgggg tgatgtttga cgatccgagg gcgaatcgtg accgctgggt
     4021 caatcgggcg tgattcgttc gtcaacgatc gtcgcacctt gaccgcgccg cgtaacgcag
     4081 gagcggaccg cctcgcgcag ccgtcccggg agtaccggct tcgacagcgt ttcatgcacg
     4141 gccgggaaga gcgaccggat ctcgcgaacg tcgagcccgg taaccacgat gactttgccg
     4201 agaaggcgcg ggcgatggcg gtggagagac tccaacacct cgataccgct gaccttcggc
     4261 aggacgatat cgagaaggat gacgtcgtac tcctggtcgc cgaggaagcg gagagcctcc
     4321 tcgccatcgg cggcgagatc gaaggcgaag tcgagagatt ccagcaattg tccgagaagc
     4381 ccgcgggcag cggcatcatc ttcgacgatc agggcacgtg atcgcaggtc aatcattcag
     4441 gagcgctatt ctagccataa atgcctccgc gtttgctacc gccagggcca gattctcctt
     4501 ttctgtgact cctgcgctcg cccgcggctt cagcgaatgg tcgccatccc ggagccattc
     4561 gacctcgatc gacggcgata gaagatacgt tgccacgtcc tcgcggtttc cgaagggatc
     4621 gcgctcccct tgcagtacga gcatcggcgt gctcagcgtc tcgaggtgcg cggtacgcac
     4681 tttcgccggc tgtccgggcg gatggaacgg atagccaaaa cacaccaggc ctcggacccc
     4741 caattcatcg gcaaccatgg tcgccatccg cccgcccatc gatttgccgc cgatgaatag
     4801 ccgctcacgg ttctccagcg tttcaacgac gctgcgccag gtagccagca gaaccggctg
     4861 gcgatcggga gcgccgcgct ttccgcccgt ccgccgcgca gccatgtacg ggaattcgaa
     4921 ccgcacgaca cggatgccgc gagcgccaag cgcccgagcc accgtcgtca tgaacggcgt
     4981 gtccatcgct ccgcccgcgc catgggcgaa ggcgtaggtg atcgcagcgt cttccggacc
     5041 gtcgaagagg aacattgctg cgaagcttac aatctcgcga tgcgatttcg tctcttcgcc
     5101 tttgctctgt ccgtcgcttc gattgcctcg atcgcgtccg cggccactcc tccgccgaac
     5161 acgatcgctc caccaaccat cgcccatccg atggcgatgt tcctcggcga gctctcacac
     5221 gacggcaagc agaaagtaac ctttcgcgcc agcgccctcg gcacacactt tttcctcgag
     5281 gaagcggctg gggtcacggt ctacgtgtac gacgagaccg tggggtatcg gaaggagacg
     5341 tttctcaagg gatcgacgct ggcgaaggcg gtgaagaagt atcggtagtg cgcttcgcgc
     5401 gttgtcggtt gtcggttgtc cgttagttgt ggcactggcg caggcgtttc gcagaacggg
     5461 taacggaaga ccgacaaccg acaacgcggc gaagacgcag ctaaggcctc aatccccgat
     5521 cactccggca gagtaacccg cctcgataac ccgagccccc agccgaatgc ccccgccccc
     5581 tcgaaatcgg agccgctccc ttcgacattg gtaacgttcc cttcgacatt gacggcgctc
     5641 ccttcgacat tagaaacgct cccttcgaca ttgacgacgc tccgtttaac attagaaacg
     5701 cgccgtttga cattgacggc gcgccgtttg acattagaaa cgcgccgttt aacattgacg
     5761 acgggccgtt taacattagc gccgggccgt ttaacattag cgccgggccg tttgacatta
     5821 gcgccgggct gtttgacatt agcgccgggc tgtttgacat tagccgcgct ccaatgtcta
     5881 gggctgcgtc gcccatgtca ctgacaattt ttgtcacttc actcagcgac cccagcgaca
     5941 catacccgct aacccgcatt tctcacacct ttgaagaatt cggcgctccc ttgcatcgag
     6001 cgccttctcc ccccgggctt cttcgggggg agaaggtgcc gaaggcggat gaggggggcg
     6061 aacgcccgac aagcgatgtt ctctcgcaaa aggctgattg acaggtctgg ttttatcccg
     6121 tcatgaaagc ctcgctgcgc cagggaacac cccctcatcc ggcttcgcca ccttctcccc
     6181 cacaaaaaag cgtgggggag caggcgctcg attggaaata gtcgcgatta gagtgtgaga
     6241 aaaaacgggc taaccgacac gttttgacgg tatcgtcaac ggcttcaacc ccccccaact
     6301 ccccaccccc tacggaacca atccccgatc actgcggcag agcgtttcca cgcgcccgtc
     6361 gtcgccgacg acgagggtgt cttcgatgcg cacgccgatc tcgcggtcgg ggaagtagag
     6421 gccgggctcg atcgtgacga tgtcgccgcg ctcgatctcg tcgccgttcg acggagagag
     6481 agagaacgac ggtttttcgt ggacgtcgag accgacgccg tggccgaggg agtggacgta
     6541 gccttcgagg gtggcgggat tcgagcgcgt cgtcgcatag ccgcgcttct cgaagaagtc
     6601 gcagaccagc gcctggtatg acgaggcgaa ggtgccggcg tgcatctcgg cccgcgccag
     6661 ctcgaacgcc gccagaacgt cggcgtgaat ccgatgcagt tcgtcgggga tggggcccac
     6721 gcaaaacgtc cgggtgagat caaagaagta gccggtggcg cgatcggcgg gaaagatgtc
     6781 gaggacgatc ggcaccgagg ggcgaacgac cgcctccgcg tcgccgcgtg aatgcgggat
     6841 gccggcatcg cgtccctgcg agaggatcgt gtcgtgatct tccaccatgc cgcgcgccgc
     6901 gatctcgtgc gagacgaggc gcttcagatc gccgagcgtg agcggacggc cgtcgtgaac
     6961 gagctgacga tcactgatcg tcgcgttgcg cagaacctcg cgcacgcaat cgacgacctg
     7021 ctccgtgcgc tcgccggcgg aacggatggc ctcgacttcc cacggctcct tgcgcttgcg
     7081 cgcgagttgg acgagatcgt cgctgcctcg ccagacgctc caaccgcgcg ctccgagccc
     7141 ttcggcgatg ccgaggtaga gcgggaaggg aagattgcca acgatggcga cggtggattt
     7201 ggcgccgagc tcgcgcagca cggcatcgaa gaacaaccca tacgcctcgg cggcattcgc
     7261 tgccgacttg aagatggcgt cgtaaccaaa gtcgtgaatg aggcgcgtgg tcaagccggt
     7321 ggcggaggct tcgtcgcgct ccatcggata cgagatgagg atcggatcgc ggccggcgag
     7381 cttcacggca tagccgcgcg tcactttcgc gccgcgtgtg atccagcgaa acgaaggatg
     7441 catcgcctcg tgcatcggca cgaggagggc gtcgagctcg cgctcacgca tcaggaggtc
     7501 aaggtctttg agcattggtt gattgcgctt cgcgcgttct cggttgtcgg ttgattgcgc
     7561 tgtcgcgcgt tctcagttgt cgggatgcgg gccgtcaaaa gaagaacgtg ggcgctctca
     7621 ttttattcaa tgcgagttac cggttcgcag ttttcggaaa tgcctgggcg acgactgcgt
     7681 cgctggcgag ctacaatttt tccggcgtga ccatgagcaa gcgcaggcga gcccgccagg
     7741 ctcagaaaaa gttggataag aagcgagcgc gtgctcgcag ggcccctgtg ctggtcgcag
     7801 caccggtggt gccctgcatc gctcccgacc cactggaggc agctgccgcg attctcggcc
     7861 gcgttgcgga agacttgatg aatgctcatc agattctcga cgacgtaagc aacatcggcg
     7921 tgtggtcaaa gtccgacgac tgcagcgaca accgccagag acgcaaaggc ttgcgggagg
     7981 ccggctcggg cgtgtcgacg gcgagaaggc ggctcacgga tctcgagacc accgaacccg
     8041 agcttcagcg cgacttcgcg ttgctcgacg ccgacattcg cgccgtggtt gcggatctgg
     8101 agcctttgcc cgacacggtg agcgacatca tggacaggaa tccggcgttg gacgtgagta
     8161 tctatgatgc gatggcgcgc atcgacgcgt tgcgcgaacg ttcgctgggg atctcgtcgg
     8221 aatacacccg gcgcgctcag ctcgcgagac agcagtagcc ttccggcggt tttcctgcgg
     8281 accgggttac ctcagttcga caaggtccca cagattgccg tacaggtcct gaaacaccgc
     8341 cacggttccg tagggctcct ccttcggctc gcgaacgaac gtgacgcctt tgtcccgata
     8401 gttctggtag tcgcgggcga agtcgtcggt gtagaggaag aggaacacgc ggccgcccgt
     8461 ctggttgccg atgcgcgagg cctgctcctc gttggccgct cgtgccagga gaaggcgcga
     8521 ttccgtcgtc gaaccgggag gcgcgaccac gacccagcgc ttgttctgcg cgggaacgaa
     8581 cgagtcctcg atcagttcga aacccaggac tcctacatag aaggcgagcg cctcatcgta
     8641 ttcgcgcacc acaagcgaga cgagaccaat tgtttgcttc ataggaattg gaggtctctc
     8701 cgacaacgcg cgaagcgcaa cgagcgacaa cgcaaagaaa aacggccggc gaacgccggc
     8761 cgttttcttt gcaatctaga tgtttcgatc gatgaactgc tggagtgccg gtttcggcat
     8821 ggcgccgatg gcccggtcga tgagcttccc gttcttgaag atcatcagcg tcggaatcga
     8881 cgtcacaccg aacttctgcg ggacgttctg attctcgtcg acgttcattt tcgtgatgac
     8941 tacttttccg tcatacgacg gcgcgagctc ttcgacgagc ggtccgatga tgcggcaggg
     9001 accgcaccac ggcgcccaga aatcgacaaa tacgggctgg ccgccgttca cgagctcttc
     9061 gaaatcctga tcactaatct ctcgtactgc ctgcgaagtc gccattccag atctcctgaa
     9121 ccggttgaaa gctgacggtt tcatgcctta ttccatttcg cgcatgtgtg cgaccgcggc
     9181 aggagtggcc ttgcagctag cgctacatct tcgaacgccg cgagacctcg taccaagccg
     9241 cgagcaagaa cgccggcgaa acttcgtccg ccgccctgac agccctcggg cgaggacgcc
     9301 agagggcccg cggtcgagac gaccgcactc ctgctggcac gtccctcgct ctaggagtcg
     9361 gtaatgtccg atcctcgccc gcatgggcat gaacgccggt cgttcccgcg gccgcctctc
     9421 tggttgaacc tccttctttt gatcatcgcg gcaggcacgt ttgtttatgc caagcaccag
     9481 cgcgacgtca tcgaagagaa gaccgccatc ctctttcgcc acaccgcgaa ttcgcccgtc
     9541 gaactgaacc ggatgcgcga tgagctctcg cagatggatc ttacgcaggc gcagctcaag
     9601 cacgagatcg acgcccgcat gcagtatctg caaagccagc agagcgcgca gttctacatc
     9661 tccatcgaca cgcagcggaa gaagatgcaa ttccgcttcg ggcgcgacat cgtccgcgag
     9721 acggacgtgc agatcggcga agcaaagacg atcaagtcac ccaccggcaa gacctggacg
     9781 ttccttccgc tcaagggcgg cttcagcgtc gccggcaaag aagacggcgc ctcatggccc
     9841 gtccccgaat gggtctacgc gatgcacggc gagcgcgagc cggccgagcg gcgcgtcgtt
     9901 ccgaacggcc tcggacgcta cgtgatcttt cttcaggaca gctacgtcat tcactcccca
     9961 ccgccgccgg agagtccgtt gcagggaccg aaaccgggat cgttcatggt ccccgaggcg
    10021 gatctggcgg cgatctggcc acggattacc aatgacacga gggtctacat cttctaatga
    10081 aacgctggat cggattctta ggctcctggg cacttctcgt actggcattc gggctggcgg
    10141 cattcgccgt agccggcgaa acgatcaaca tgcgcgacga acgccgtctc gaccgcgtcg
    10201 ccgagatcga tcgcctcatc aacgaagatg gcggcagcaa agcgagcgtc ctgcgcaaca
    10261 ccgcttcgca gaaaactgcg aacgtgaacg cgttgcaatc gaagttgcag gatgtcgaaa
    10321 agcagacgga tgacgttcct gacaccggcc agaccattct cgtctcgact gccgagaaca
    10381 agctttacgt tcgccgcggc ggccagacga ttttcgaagc cgtttgctcg accggcaaag
    10441 gaacgacgct cgccgtagac ggcaagacga ttctgttcga cacaccgatc ggaaagcttc
    10501 acatcatctc caaggacgag aatccgcaat gggtgccccc cgactggcat tacgtcgaag
    10561 aggcgcggaa gaacggcatg cgggttgtcc atctcgaccc cggtcagacc atcgatgcgc
    10621 gcaccgggca gcccgtgtcg agcaaacccg atgcaggcgt ctggtcatgg ctgtcgggcg
    10681 gcaataacaa cagcaccagc aacgacgtac tcaaggtgcg aggcgacacc gtggtcgaag
    10741 tcaacaacgg tgtcgaacgc gagctcgagc ccgggaagac gatcgttgcc ggtgatgccg
    10801 tcatcgtccc tcccgtcgac accaagcagc gccactacga caaagtcctc ggcaagttcc
    10861 gtctggagat gggcaacggc tacggcattc acggcactga cgagccggac aagctcggac
    10921 aatccgtctc gcacggctgc gtgcgcctcg gcgacgcgga catcgaaaag ctctaccaga
    10981 tcgcgaacgt gcacgacacg gtgatcattt attgagggca gagatcaggg aagacagaga
    11041 tcagggaaga cagagatcag agacaacaga gactagagat cagaggtcgg cgtcgggatc
    11101 agcacttcag tcctgatctc tgatctcttg tctctgatag tctctgatct cttgtctctg
    11161 atctcttgtc tctgatctct tgtctccgac ccccgactcc cgactcccga ctcccccgct
    11221 tacaatctca tcatgcaccc ctggcgcgag cggcttgctc gctggttcac accgctggcg
    11281 cggcggatgc cgctgtctcc gaacgcgatc acgctcatcg cgttcgcgct gaacctgatt
    11341 ggcgcggcgt gcctggcgta cggcggggag cggccgccgc tcttcctcgt cgccatcatc
    11401 ttcatcgcca tcagcggatt cgccgatgcg ctcgacggca tcgtggcgcg cgtgcagaac
    11461 aagacctcgc gcttcggcga ctttctcgat cactgcgccg accgcgccgg cgacacgctg
    11521 cttgccgctt gctggcttta cgggaacaac gtgcgccagc cgctggcgat ggccggcatc
    11581 atcatgacga tgctcaacgg ctacatcggc acgcagatcg aggcgacgta cggcgagcgc
    11641 agctacgagt ccatcggccg cggcgagttc gtcctcggca tcatcattct tccgatcgtc
    11701 tcgtacagcc tcttcacgaa cggatggcag gcgctgcaat tggcgaggct gacggtcgcg
    11761 gaatggctgg ccgtggtggt gctgctcttc gcgctctacg gcgtcgtgca gcgcgtgcgc
    11821 gtcgcgattc ggctggagcg ttcgtgacgg acgagttgaa tccgatcaca ggcgagccgg
    11881 tgatcgtcgc gccgcaacgc gaggaccggc ccaatgtcta cgagggcgct ccgtgcccgt
    11941 tctgtcccgg tgccgaaaac gaaacgccgc cggagatctg ccgcgacggc gatccgtggc
    12001 ggatccgggt ctttcccaat cggtatccgc cgacggagca ctccgagatc atcgtcgaga
    12061 gcgcgctgca cgacgacacg tttgacgcgc tcgctcctga tcatgccaag cgggtggttg
    12121 agctctattt cgagcggtat cgcgcgctgg ccgcgaagac agcgtacgtt tgcatcttca
    12181 agaaccacgg tcgtagggcg ggcgcgtcga tgccgcatct ccattcgcaa ctggtaggga
    12241 cgccattcat cccggcgcgc atcgcgcgag aaagcgctgc attcgcggaa gccgctaact
    12301 gtccgctgtg cgatctgcgc gaacatcctc tgatcgtcga gtccgagcac tatcgatgga
    12361 ttgccccacg aggcgcgcgc ctcgcctggc agcaatggat cgttcccaaa gcgcacgaga
    12421 atcaactcga cgaaccgcac gagctggcgt cgctgctcca gtcgtcggtc cgcgccatga
    12481 gcacgatcag cgattccttc aactgggcgt ttgtcgtatt cccgggcgag ccgagggcgg
    12541 gacattggta tctcgagctc ctcccgcgca tcggcgcggt cgcaggcttc gagctcggga
    12601 ccggaacgtt cgtcaacacc gtctccgctg acgacgttgc cacgctcttt tcgcgcttca
    12661 agtgaagatg cctcactaaa gacgcccgtt gcgccaccga tagtcctgag gcaccggacg
    12721 caaaggcggc cggcgcagac cgccgagcag gacgaccagc aacatcccgg cgatgaagcc
    12781 tccgatatgc gcaaagtagg caacgccgcc ggcgctctga agatcgccgt tcatcgacgc
    12841 cgcaccgaag aagacctgca cgacgatcca gaagccgagg aggaagacgg cgggaatctc
    12901 gcggacggtg atgaagatga agatcgggat cagcgtcgtc acgcgctgct tcggaaagag
    12961 gacgatgtac gcaccgagca cgcccgcgat cgctcccgac gccccgatgg acggcacgcg
    13021 cgaggtggga ttcattgccg cgtgcgtcag cgtggcgatg agcccgcagg cgatgtagaa
    13081 gaagaagtac ttcacatgcc cgaggcggtc ctccacgtta tcgccgaaga cccacaggta
    13141 gagcatgttc ccgccgaggt gaagccagcc gccgtgaagg aacatggaaa cgaacatcgt
    13201 gatgatgttc ggcaggagcg aatcgggcag ataccagaag cgcgccggaa cgaaggcaac
    13261 gttggttaaa accggctcga attgcggccc gagggagatc tcccagaaga acatcagcac
    13321 gttgatgacg atgaggatga tgttgacgac cggcgtcgtc cgtcgaggat tgtgatcggc
    13381 gagcgggagc aagggggaat tatgcgcttc gcgcgttgtc ggttatcggt tggttgcgct
    13441 tcgcgcgttt ggggccagcc ggattcggcg acgatttctc gttgtcgcgc ctggtatccg
    13501 ctgtcgcgtc cgtccatccg cgtcgtcctg cttgtcaccg ccaagtactt gacttgtgat
    13561 cactcaggcc ataaattgga tcagactgtt cccgatcgag gtcacgatgc gactctttac
    13621 ttccattgct tgcgtcaccc tgcttgccgg ttgcttcgcc atctcgcgac acacgccttc
    13681 gctgatcgtc gccgcgcgcg agggcgacgc tccggcaatc cgcgcgatcg tcaagagagg
    13741 ttgcgacccg aacgctgcat acggtgcgct caactggacg ccgctcatgc acgctatctc
    13801 cagaaaccag atcgaggccg tgaaggcgtt gctcgattcg ggcgccgacg tcaacaaaac
    13861 gggcgattac gaaacggagc cgcgcacgaa gcagcttcgc gtcacgcctt tgatgatggc
    13921 agcaggctac ggctacacgc ccatcgttga gctgttgcta caacgcggcg ccgatcccac
    13981 gcccgccgac agtgacggca atcgcgccct ggactacgcg ctgaccggca cgctctatcg
    14041 ccgcactctc ttcgaatgcc aggacagtac cgttcgcgcg ttgctcgcgg cgcacgctcc
    14101 ccggagcgtc actgctgact cgcgcaagtg ggctgggatc aagcgttgtg agactcttgc
    14161 gcttttgcga tgaaaaacga tcaccacgga gacacagagg acacagaggt tttctctgtg
    14221 ctctctgtgt ctctgtggta gttcctctaa tccagcgccc tgagtaaatc ctccaccaga
    14281 tcatccgcgt gctccagacc cgttgagatc cgcaggaggt tctccgcggt ccggcttccg
    14341 gggccttcgc tcgagttgcg atgttcgatc aggctttctg tgccgccgag ggatgtggcg
    14401 cgtgtgaaga ttttcgtctt ggcggtgacg gcgagcgccg cctctcgccc gccccggacg
    14461 ttaaaggaga gcatgccgcc gaacgcggac atctgcttcg cggcgatggc gtgaccggga
    14521 tgcgattcca gtcccggata gtgcacggct tcgacgcgtg gatggtttgc caaggcgcgc
    14581 gcgagggcca tcgcgttctc gcaatgcacg cgcatccgcg cagggagcga acggatgccg
    14641 cgcaggatca gccatgaatt gaacggcgac gccacgcccc cggtgatctt gcggcagtgc
    14701 tcgaccgcct cggtcagcgc accacgctgc gcgaaggcga gtgaaccgcc ctgcacgtcg
    14761 ctgtgtccgc cgaggtactt ggtggtggag tggagaacga cgtcggcgcc ttgttcgatg
    14821 ggacgctgca acgccggcgt ggcgaacgtg gcatccgcga tcaggatcgc attgccctcg
    14881 tgcgcgatgc gcgcgagcgc ggccacgtcc gcgattctca tcaatggatt cgatggcgat
    14941 tcgcaccaga tcgcgcgcgt cgttgggcgc accgccgact tcaccgcatc gagatcgtcc
    15001 atcgccacga agtcgaaggt caggccccag cggttcagga actcgttggc cagcgcgcgc
    15061 acgccgtagt aactctcgtc ctgcagaatt acgtgcgagc cggctggcag cgcctgcaac
    15121 aggcacgtcg tcgccgccat ccccgaggcg aaggccagcg cgtgcgatcc cgaatcgatc
    15181 gccgcgagcg cctcttcaag acgcgcttgc gtcggattcc cgtcgcggat gtaactgaat
    15241 ccgtgcgacg gctcgccgtc ctcggcgcgc cgctcaaatg tcgtcgaaag atgaatcggg
    15301 gccgcaattg caccagtttc gtcatcagcc cctgctccgg aatgaaccgc ctttgtttcg
    15361 aatcgcatgg cggaaggata aactgggagc actcatccaa cgtattcagg ttcccatggc
    15421 acgccggtcc gcgtttctgg cagttcttgt gttcatgact gcggcagcag cactcgccca
    15481 gcccgtgatg acctatgttt acaacgctcc tgaatcggcg ctggacgtgc gctatcagta
    15541 tcactgggag attttgagaa cggcactgga gaagacgacg ccgaagtggg ggccgtaccg
    15601 gatggtgaag tcggagttga tgaccgagcg ccggcaggcc ctcgagctga agaacgccac
    15661 cggcaagctg acggtgatgt atctgagtac gactccggag ttcgagcgca gtctcatccc
    15721 gatccacatc cctgtcgaca agaacctcgg cggctactgc gtcttcctga tccgcaagga
    15781 ggagcagccg cgattcgcga acgtgcgcac gctcgccgac cttcacaagt tcacgttcgg
    15841 actcggcctc ggctgggtcg acgtcgacat cctcaacgcc gcaggcctta aggtagtcac
    15901 cggatcgagc tacgacggtc tgttcgagat gctggccagg aagcgcttcg acatctttct
    15961 gcgcgccgcg gtggaggtgc tcgacgagta cgatcagcgc aaaagccgtc tccccgatct
    16021 tgatatcgaa aggaacctca tcctctacta tcccctgccg atgtacttct ggtttccgaa
    16081 gacggcggaa gggcggcggc tggcgacgcg tgccgaggaa gggatgcgga tgatgatcgc
    16141 cgacggaacg tatgaccgca tcttcgacaa gtatcagcgg cacaagatcg agcgtctgcg
    16201 tttgaagagc cggcagatca tcaagatcgg caatccgttt ctcggcccgg aagttccgtt
    16261 cagcgacaag cggctatggt tcgatccgca aacgtacaag taatgcccgc acgcagatcc
    16321 tcgctcgcca tggtcgttgc cgccacgctg gtcgcggtga cgactgtgtt gctcggcata
    16381 tacgggatca tcagctatcg gttggacgca agggcgcaac ggaaggcgct ccaggacctc
    16441 accacgatcc aggcaaacga gctttccgta gcactggcgc tcccgatctg gaacgtcgat
    16501 cgtgcccaga tcgataaggt gatcgaggcg atgacgaaac cgaagtcggt ctccggaatc
    16561 tccgttcata cagcgggcga ggacattggc cgcctgcgga aaccggacgg gaagttcagg
    16621 ctgtgggatg gaaaatccac acccgccgac atgctcgtcg aggagaggga gatctacttc
    16681 ggcgacaagc agatcggaac cgtgcgcctg ctcgtgacga aggaatacct taacaccgat
    16741 cttcgcatgc tgctgattcg catcgtgggc atgatcgttg gggtcgattt gctcgttgtc
    16801 ctcggcacgt acttcatcct tctgcgcgtt gtggttcgtc cgctgaacga gatcgaacgg
    16861 tatgccgttg ccgtcagcac gggagtaggg ccgcaaaacg cgccgcagcc ggagtcgctt
    16921 ttcacggctg atctggagaa cctccgttcc agcattgcca aaatggttct ccagctcgag
    16981 cagcgttaca ccgaattgaa ggagcaggaa gacttcacga acacggcgat caacaccatg
    17041 accggcattt tcttcattca ggaccgtgag gggcgattca ttcgctggaa cgacaacatg
    17101 gctcgcgtgg cagacggcga gcagcttctg tctgaccgcg tcggccgtcc aatggttcac
    17161 ccggatgacc gcgcgatgat cgaagagaaa gtcgccgaga tattcgatac cggtcacgcg
    17221 gaggtggaga cccgcatggt gctcaaagcc ggcatcagga actacctgtt caacgcgagg
    17281 cggatggacg tccgcggcca gccattcatg gtggcaaccg gcgtcgacat cacggagcgc
    17341 cggcaagccg aggcggagca gcggcggttg cagagcgaga tcgcgcgctc cgcggccgaa
    17401 tggcaggaaa cgttcgacac cgttaccacg ccaattctga tcaccgagga gaacggagcg
    17461 atcgtccgcg tgaaccgcgc ggcgctggag ctgagccgct tgtccgagga gcagatcaaa
    17521 gggaagccga tcgggcaact cccatcgcgc gagccttggc acaccgccgg acagctcgtc
    17581 tcctacatca ccaccgaggg ccacggcacg tccgcggaaa cgaaggacgc agaaggcagg
    17641 acgtgggaca tcgtcgtctc ccacttctcc gctccgaacg acggtggaga gcgcttcatc
    17701 ctcgtcctgt gggaaatcac cggcatcgtg gagctgcagg aatcgttgcg gcgaagcgag
    17761 acgctgtcgg cgatgggaaa cctcgtcgcg ggcgtcgcgc acgaggtgcg caatccgctc
    17821 ttcggcatct ccgccacgct cgatgcctat caggacgaga tgagcaggcc gggctacgtg
    17881 gagttcgcgg ccacgctgcg gcaggaagtg aaccggctga ttcacctgat gaaggaattg
    17941 ctcgagtacg ggaaaccagg cgcgctgtcg atcgagcgcg ggcatctccg cgacgttgtc
    18001 ggcagcgccg tcgaaagccg gcagcaggcc gcacgcgccg cacacgttcg catcgtgaat
    18061 caaatgacca acggcatccc gacgctgctg atggactcct cgcggttgcg ccaggttttc
    18121 gagaacctga tcgacaacgc catccagcat tcggagcgcg gcagcaaggt tcagatcagc
    18181 agctcgatcg tcgagcacgc aggccgtcac tgggtggagt gccgcgtcga cgatgagggc
    18241 accggcttcg ctactgagaa cctcgagcgc gtcttcgagc ccttcttcac gatgcgcgac
    18301 ggcggcacgg gcctcggcct ctccatcgtg catcgcatcg tcgaagagca ctccggaaag
    18361 gtattcgctg cgaaccggcc ggagggcggc ggcagtattc gtgtgctctt tcccctcggc
    18421 ggtaccgagg ccgccgcgaa aagtgtcgca tcatgacgga catgactcga aaccgcgtgc
    18481 tgatcgtcga cgatgaaccg tcgatccgga tggccattcg ccgctttctc accacctcgg
    18541 gattcgaggt ctcggacgcc gacagccgca gctccgcgct ggcgctggcg cgcaaggagc
    18601 ggcctgacgc gatcattctc gattacaagc tgcgcgactg caccgcgctg gatctgctgc
    18661 cgcaatttct ggccatcgac gccgaggtcg ccatcatcgt gctcacggcg cacggaagcg
    18721 tcgagctggc cgtcagcgcg atcaaggaag gcgcgcagca gtttctgacc aagccggtcg
    18781 agctgccgac gctcctggtc atcctcaacc gcaccctcga cacgcagcgc agccggcagg
    18841 ccgatcaggc cgatcggaag aaagagtcgc gcgacgttct cgatcccttc accggcacga
    18901 gcgccgcggt gcgccagctc gaatccgacg ttgacaagat cgtctccacc gaccggccga
    18961 ttctgattca aggcgagacc ggaaccggaa agggaatcct ctccaactgg cttcatcgca
    19021 acggccggcg gaagcaggag ccgttcgtcg atctgaactg cgccggtctg tcgaaagact
    19081 tcctcgagtc ggagcttttc gggcacgaga aaggcgcgtt cactggcgcg gtgcaagcca
    19141 aacagggtct cttcgatctc gcgcaccgcg ggacgctatt tctcgatgag atcggcgacg
    19201 tcgatctgca ggtgcagccg aagttactca aagtcctcga agacaaacgc tttcgccggc
    19261 tcggcgatgt gaaggaccgt ttcgccgatg tgcggttgat cgcggcgacg catcacgaca
    19321 tgaacaatct ggtgcgcacc agacgctttc gcgaggatct ctacttccgc atcagcacga
    19381 tcgttcttcg tttgccggcg ctccgcgagc ggccggagga catcgaagcg ctggcggccg
    19441 atctcctcgc gcggatctcc cacgagctgg gccgctccgg aacgagtctg gccgctcctg
    19501 ctgtccgcgc gttgcaacaa tacgcgtggc cgggcaacat ccgcgagctg aagaacgtgc
    19561 tggagcggtc gctccttctg agcgagcgaa acgagattca gcgcggcgac cttcgcttcg
    19621 aacatccgac gtccgagggg gcggcggagg atacgcggct gacgctgacc gaggtcgaag
    19681 cgcgtcacat cacgcgcgtg ctgaaagaag aagacggcgt catcgaccgc gccgccaagc
    19741 gcctcggcgt cacccgaaac acgctctact acaagatccg caagcaccga atcgcactgc
    19801 gcaagaatgt cggcgcggat gccagcgaca gcgaaagccg cgcttaatca gtcacgcgaa
    19861 gacgcgaaga cacgaagctg cag
//
LOCUS       DATLVW010000596         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_522314, whole genome shotgun sequence.
ACCESSION   DATLVW010000596 DATLVW010000000
VERSION     DATLVW010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522314"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VNN25_17645"
     CDS             complement(<1..721)
                     /locus_tag="VNN25_17645"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXH93412.1"
                     /translation="MIETISRDRLIELLEAGANQRIAIIGDAMLDVYLRGDVDRISPE
                     APVPVVRVRDKELALGGAANVAQNIFAIGAQCDLVCAVGEDAEGQVIKAMLRDIDSDG
                     RSVVTVGRRTTTKTRVLARSQQVVRFDEEEDTDITGDEVAGLLDAAVRAIDNADALVL
                     EDYNKGVLTPVLIGPVMHQATARGMPIVVDPKYRNFFAYKGATIFKPNKRELESALGA
                     AVDLEHPEALPESIKRLGVEHL"
     gene            1051..1857
                     /locus_tag="VNN25_17650"
     CDS             1051..1857
                     /locus_tag="VNN25_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93413.1"
                     /translation="MILESFNDAAKRGVHSLQEFFVLAGKALGFIFVKPFYTADLFQQ
                     MDAIGVGSIGIVLLTGFFTGMVLALQSAVQLQTFGATDYIGRLIAGSMIRELGPVLAG
                     LMVAGRVGSGIAAQLGSMRVTEQIDALNTLGTDPIKKLVTPRVLASLIMLPMLTVIND
                     FVGILGGSVIAKFYLGKPASLYWRTVFEQILSGGFTLKYIPNDFIQGLTKPFVFGGII
                     SLVACYHGLKTTGGTEGVGNATTRTVVAASILILVVDYFLTQILLAVFQP"
BASE COUNT          376 a          641 c          607 g          394 t
ORIGIN      
        1 gcaggtgctc gacgccaagt cgcttgatgg attcggggag cgcttccggg tgctcgaggt
       61 cgacggccgc tccaagagcg ctctcgagct cgcgcttgtt gggcttgaag atcgtcgcgc
      121 ccttgtaggc aaagaaattc cgatacttgg gatcgaccac gatcggcatc ccgcgtgcag
      181 tcgcctggtg catcactggc ccgatcaaaa ccggagtcag cacacccttg ttgtagtcct
      241 cgagcacgag ggcgtcggcg ttgtcgattg cacgcaccgc cgcatcgaga agccccgcta
      301 cttcatcgcc ggtgatgtcg gtatcctcct cttcgtcgaa gcgaacgacc tgctgcgacc
      361 gggcgaggac cctcgtcttg gtagtcgtcc gccggccgac cgtaactacc gatcgtccgt
      421 ccgagtcgat gtcgcggagc atcgccttga tcacctggcc ttcggcatcc tcgccgacgg
      481 cgcagaccag atcacactgc gctccaatcg cgaaaatgtt ctgcgccacg ttggcggcac
      541 cgcccagcgc cagctccttg tcgcgaaccc gaaccaccgg aaccggtgcc tcgggagaaa
      601 tgcggtcgac gtcgccgcgg aggtagacgt ccagcatcgc gtcacctatg atcgcgatgc
      661 gctggttggc gccggcttcc aggagctcga tcagtcggtc tcgggagatt gtctcgatca
      721 cggagttatt ttgcgagtaa ttgttaactg caacggaacg ggcgcgaggc gacagctgga
      781 gagttgtcag tctttttagt ttactacgcc ctgacttatc cctgtgctgt tcatgtgact
      841 gctcgtgccc agaacgccag tagtagagtc agggcgtcgt gaacttcaaa ctggcatctg
      901 cgtagctgac gcctctcgcc cgttccgttg cagatttcac gcgcttcgac aagcccaagt
      961 taatgcgggg ccaaccgcga gcaacgggcc accccttgcc cggtgggtgg gtcaagcggt
     1021 acgctccgat cctaccatga cgccaattat ttgatcctcg aaagcttcaa cgacgcggcc
     1081 aaacgtggcg tccactccct ccaggagttc ttcgtcctgg ccggaaaggc gctcggcttc
     1141 atcttcgtca agccgtttta tacggcggat ctcttccagc aaatggacgc gatcggcgtc
     1201 gggtcgatcg ggatcgtgct cctcaccgga ttcttcaccg ggatggtgct cgcgctacag
     1261 tcggcggtgc agctgcagac gttcggggcg acggactaca tcggccgact catcgccggc
     1321 tcgatgatcc gcgagctcgg gccagtgctt gccggcttga tggtcgcggg acgcgtcgga
     1381 tcgggaatcg ccgcgcagct cggatcgatg cgcgtcacgg agcagatcga cgcgctcaac
     1441 acgctgggca ccgacccgat caagaagctg gtgacgccgc gcgtcctcgc atcgctcatc
     1501 atgctcccga tgctgaccgt catcaacgac ttcgtcggca tcctcggcgg cagcgtcatc
     1561 gcgaaattct acctcggcaa gccggcatcg ctttactggc gaacggtatt cgagcaaata
     1621 ctgtccggtg gattcacgct caagtacatc ccgaacgact tcattcaggg attgaccaag
     1681 cccttcgtct tcggagggat catctcgctc gtcgcctgct atcacgggtt gaagaccacc
     1741 ggtggaacgg aaggcgtcgg aaacgccacc acgcgcaccg tggtggccgc gagcatcctg
     1801 atcctggtcg tcgactactt cctcacgcag atactcctcg cggtattcca gccatgaccg
     1861 acgacgaaga cgatcgccgc cgcaaaggcg aggagcggcg ccacccgagc gagaaagacg
     1921 aaacctcgat gcctcgccgc gcgcgcgact cgggacgcgt gcgcgctgcg atcaggaacg
     1981 agctggagga agtggccgag gacagcgcgg caaaggtt
//
LOCUS       DATLVW010000597         5336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_561513, whole genome shotgun sequence.
ACCESSION   DATLVW010000597 DATLVW010000000
VERSION     DATLVW010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561513"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VNN25_17655"
     CDS             <1..274
                     /locus_tag="VNN25_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXH93414.1"
                     /translation="REQRIIRIHGKVQGVGYRFFATRVARRLGLKGSIENSRDGYVEA
                     IVEGEKGVIDEWIEELKEGPRYAEVTSIDQESKDFTGRLGDFDVKF"
     gene            274..786
                     /locus_tag="VNN25_17660"
     CDS             274..786
                     /locus_tag="VNN25_17660"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXH93415.1"
                     /translation="MDLRPYIRDVPDFPKPGIVFKDITPLLRSHAALDYVSHSLAEPF
                     MDAGITSVVAIESRGFIFGSGVARVLGAGFVPIRKPGKLPAKTRRSEYTLEYGSGILE
                     IHDDALTADDRVLIIDDVLATGGTAACSVTLVRECGATVVAAGFVIELTFLDGRTKLD
                     GTPVHSLLAY"
     gene            1005..2417
                     /gene="sthA"
                     /locus_tag="VNN25_17665"
     CDS             1005..2417
                     /gene="sthA"
                     /locus_tag="VNN25_17665"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HXH93416.1"
                     /translation="MYDLIVIGSGPAGEKGAAQAAYFGKSVALIEREPVLGGACVNTG
                     TIPSKTLRESALHLSGFRQRGFREGSIEVTINCITVADFMYRKDRVVAHEWKRIDDNL
                     RRHKIDRYEGDARFVSPTRIAIKNAVGEEVIDGSVVLIATGSSPHRPDHVPFDEDVIC
                     DSDTILGIDRIPERMAVVGAGIIGVEYASIFAALGVEVHLIDGRTGLLPNIDREIVRI
                     LLGEFQNRLGITMHLGMDVDSIERCDHQASLTLKGGECMVVDNVLYAAGRSSNVEGLN
                     LDAVGVKTGARGLIAVDENYQTSVPNIYAAGDVIGFPALASTSMEQARVAMVHAFNLK
                     YKTRVASILPYAIYTIPEVATVGLNEEDCRKAGTACAVGRAYYRDNARGEIIGDLKGL
                     IKLVFDPESLKLLGVHIVGENASEHLHIGMMVMQFNGTINAFIDCVFNFPTLSEAYKY
                     AAYDGLGSINRKRTLEQELV"
     gene            2414..2854
                     /locus_tag="VNN25_17670"
     CDS             2414..2854
                     /locus_tag="VNN25_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH93417.1"
                     /translation="MKLIGEIIQGRSLVHAKPSDSVRDVARTMNNANIGAIAVLDAGR
                     LVGIFSERDVVTRIVAEGRNPDETRVDSVMTKDLMVATPNDDINDALQKMHECNCRHL
                     PVVHRGNLVGMISIRDLLKVDDDANRAKASFLNELVTYSPDYET"
     gene            complement(3013..4122)
                     /locus_tag="VNN25_17675"
     CDS             complement(3013..4122)
                     /locus_tag="VNN25_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93418.1"
                     /translation="MRIRILFISALLIALASVLSAKEVWLSIGGTTANGTFKTDARIF
                     NPSTTKDIQIQAYYLPVGNIDNSAVQPLPPITVLKRQMVVYNDVVTSLFHSSGLGAIR
                     LKSDDDFVATQRIYAVAANGSLGQFVGGVDATSAKTKGVIIQLAAGGAFRTNVGAANP
                     NATAATVTWHLYDKNNAVVGTPFVVTMPPYAVISPSGLAGYAVGGSGADLTDAWLSYS
                     SDLPIVAYGSVIDNGTTDPTYIPASEDTGVTQSTTPSGTVFVVAEHGGTGTSSAIDIT
                     PEITSTSIKPGDTVTFRISARESTHGFRLIDPNGVILADLTVAQDAAPIERTFTMSVQ
                     GTYVYYCTITTCSPGHLNMQGSFVVGKPSQDPPGY"
     gene            4455..>5336
                     /locus_tag="VNN25_17680"
     CDS             4455..>5336
                     /locus_tag="VNN25_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXH93419.1"
                     /translation="MTTYPLEPDAAEMHRLVDSAMQRIVEHIESLPHQPASNVEGATD
                     RARTLIEPLPGSATPYDQLLDFLFDEAIPRSFNAAGPGYLAFIPGGGIFHAAVADLIA
                     NAVNRYVGVCAAAPLLAQIEANVVRWFCEIIGYGEGSGGVLTSGGSLANLTAIIAARR
                     ALLGDDFRQATLYCGDQTHHAFQKAAILAGFPAANLREIPSDAKFRVRADLLVETIAR
                     DRAAGLTPFMICGSAGTTATGAVDDLDALGKIAKSESLWFHVDGAYGAFFLLTERGRA
                     AMRGIEQADSVILDPHKT"
BASE COUNT         1019 a         1723 c         1628 g          966 t
ORIGIN      
        1 cagggaacag agaatcatcc gcattcacgg caaggtccag ggcgttggtt accgcttttt
       61 cgccacgcgc gtcgcgcgcc gcctcgggct caagggctcc atcgagaaca gccgcgacgg
      121 ctatgtcgaa gcaatcgtcg aaggcgaaaa gggcgtcatt gacgagtgga tcgaagagct
      181 gaaggaaggc ccgcgttacg cggaagtcac cagcatcgat caggaatcga aggacttcac
      241 cggccgcctc ggtgacttcg acgtgaagtt ctgatggacc tgcgaccgta catccgcgac
      301 gttccggact ttccgaagcc cggcatcgtc ttcaaagaca tcaccccgct acttcgctcg
      361 cacgccgcgc tcgactacgt ctctcactca ctggcggagc cgttcatgga cgccggcatc
      421 accagcgtcg tcgccatcga gtcgcgcggc ttcatcttcg gcagcggggt ggcgcgcgtg
      481 ctcggcgccg gcttcgtgcc gatccgcaaa ccgggcaagc tgcccgcgaa aacgcgccgc
      541 agcgaataca cgctcgaata tggcagcggc atcctggaga tccatgacga tgcactcacc
      601 gccgatgacc gcgttctgat catcgacgac gtgctcgcca ccggcggaac cgcagcctgc
      661 tccgtaacgc tggttcgcga atgcggcgcc accgtcgtcg ccgccggatt cgtgatcgag
      721 ttgacgttcc tcgatggacg gaccaaactc gatggcacgc ctgttcattc gttgcttgct
      781 tactgagggt gtggggagta gggggtgggg ggtgggagag cgaatctcga tctatctcca
      841 agtgcaagaa aagcgtttca cgcggagacg cggagcacgc cgagaaaacc tccgtggtct
      901 ccgcgcctcc gcgtgaaatg gtttgaagag ttgggtcgta gcgagaagga gacccgttcg
      961 cgaagttcga tctcccaccc cctactcccc acaacccaca ccccatgtat gacctgatcg
     1021 tcatcggctc cggaccggcc ggcgagaaag gggctgcgca ggcggcctac ttcggcaaga
     1081 gcgtcgctct catcgagcgt gagccggtgc tcggcggcgc ctgcgtcaac accggcacga
     1141 tcccatccaa gacgctccgc gagtccgctc tccacctctc cggctttcgc cagcgcggat
     1201 tccgtgaggg atcgatcgaa gtcaccatca actgcatcac ggtcgccgat ttcatgtacc
     1261 gcaaagaccg cgtcgtcgcg cacgagtgga agcggatcga cgacaacctg cggcggcaca
     1321 agatcgaccg ctacgaaggc gacgcccgct tcgtctcacc gacgcgcatc gccatcaaga
     1381 acgcagtcgg cgaggaggtc atcgacggca gtgtcgtgct catcgccacc ggctcgtcgc
     1441 cgcaccggcc cgatcacgtt ccgttcgacg aagacgtcat ctgcgacagc gacaccatcc
     1501 tcggcatcga ccggattccc gagcgcatgg ccgtcgtcgg cgcaggcatc atcggcgtcg
     1561 agtacgcatc catcttcgcg gcgttgggcg tggaagtaca cctgatcgac ggccggaccg
     1621 gccttctgcc gaacatcgac cgtgagatcg tgcgcatcct cctcggcgag tttcagaacc
     1681 gtctcggtat caccatgcac ctcggcatgg acgtcgattc catcgagcgc tgcgaccacc
     1741 aggcttcgct gaccctcaag ggcggcgagt gcatggtcgt cgacaacgtg ctctacgccg
     1801 ccggccggtc gagcaatgtc gaagggctca acctcgacgc cgtcggcgtg aagaccggcg
     1861 cgcgcggcct catcgccgtc gacgagaatt atcagacatc cgtgccgaac atctacgccg
     1921 ccggcgacgt gatcggcttt cccgcgctgg catcgacgtc gatggaacag gcgcgcgtgg
     1981 ccatggttca cgcgttcaac ctcaaataca aaacgcgcgt ggcgtcgatc ctcccctacg
     2041 ccatctatac gatccccgag gtggcaacgg tggggctgaa tgaagaggat tgccgcaagg
     2101 ccgggactgc gtgtgccgtg gggcgcgcgt actaccgcga caacgcgcgc ggcgagatca
     2161 tcggcgacct caaagggctc atcaagctcg tcttcgatcc cgaatcgctc aaactcctcg
     2221 gcgttcacat cgtcggcgag aacgcgtccg agcatctgca catcggcatg atggtcatgc
     2281 agttcaacgg cacgatcaat gccttcatcg actgcgtgtt caacttccca accctgagcg
     2341 aggcttacaa gtacgccgcg tacgacggtc tcggcagcat caaccgcaag aggactctcg
     2401 aacaggagct cgtatgaaac tgatcggcga gatcatccag ggacgctcgc tcgtacacgc
     2461 gaaaccgtcg gacagcgtcc gcgacgttgc ccgaacgatg aacaacgcca acatcggcgc
     2521 aatcgcggtg ctcgatgccg ggagactggt cggcattttc tcggagcgcg acgtcgtgac
     2581 gcgtattgtc gccgaggggc gcaatccgga cgagacgcgc gtcgacagcg tgatgacgaa
     2641 ggacttgatg gttgccacgc cgaacgacga catcaacgac gcactgcaga agatgcacga
     2701 gtgcaattgc cgtcacctgc cggtcgtcca tcgcggcaac ctcgtcggca tgatctcgat
     2761 ccgcgacctt ctcaaagtcg acgacgacgc caatcgggca aaggcaagct tcctgaacga
     2821 gctggtaacg tactcgccgg attacgagac gtgagggcgc gcccgtgcgg gggtggggag
     2881 tgggggggcg ggagtgggag cggagttccc gcaacgcatc ggcggcgtcc tcgccgccgc
     2941 tgttctcgcc ggtagtaaga cccgggcaaa gtctctacga cccccgacac ccgatcaccg
     3001 actccctcaa cttcagtatc ccggcggatc ttgcgacggc ttgcccacga cgaaggatcc
     3061 ctgcatgttg aggtggcccg ggctgcacgt cgtgatcgtg cagtagtaaa cgtacgttcc
     3121 ctggacactc atcgtaaacg tcctttcaat cggtgcagcg tcctgagcga ccgtgagatc
     3181 ggcgaggatg acgccgttcg gatcgatcag gcggaagccg tgcgtgcttt cgcgggcgct
     3241 gatccggaac gtaaccgtat cgccgggctt gatcgacgtt gatgtaatct ccggcgtaat
     3301 gtcgatggcg gaactggtgc ccgttccgcc atgctccgct acgacaaaca ccgttccgct
     3361 cggtgtcgtc gattgcgtga cgccggtgtc ttcggacgcc gggatgtagg taggatcggt
     3421 cgttccgttg tcgatgacgg atccatacgc gacgatcggc aggtcggaac tgtagctgag
     3481 ccatgcatcg gtcaggtcgg cgccgctacc gccgacggca tagccagcga gaccactcgg
     3541 cgagatgacc gcgtacggcg gcatcgtcac gacgaacggg gtgccgacca cggcgttgtt
     3601 cttgtcgtac agatgccacg tcaccgtcgc agcggttgcg ttcgggttgg ccgcgccgac
     3661 gttcgtccgg aatgcaccac cggccgccaa ctgaatgatg acgcccttgg tcttcgcgct
     3721 cgttgcatcg acgccgccga cgaactggcc aaggctgccg ttggctgcca cggcataaat
     3781 gcgctgcgtg gcgacgaagt cgtcatccga tttcaagcgg atcgcgccca gaccgctgct
     3841 atgaaagagg gaggtcacga cgtcgttgta cacaaccatc tgccgtttca aaacggtgat
     3901 cggcggcaga ggctgcacgg cggagttatc gatgttgccg accggaaggt agtacgcctg
     3961 gatctggata tctttggtcg ttgaaggatt gaagatgcgc gcgtcggttt tgaaggttcc
     4021 gttggcagtg gtgccaccga tgctcagcca gacttccttc gccgacagca cgctggcaag
     4081 ggcgatgaga agtgcactga tgaacagaat tcgaatgcgc atgtgtgatc ctccaagacc
     4141 cggcgatgat tctacatggg ttaagttgca aaagtgagct gggtcacgtg cgtaaccgaa
     4201 gtgtttctgt tcggggagtc ggaaatcggg ggtcgggagt cggagcgaga tcgcttgggt
     4261 ctcactcgta gcactcgtca agtcctcggc taccagagtc gaatcacgga acgtccgatc
     4321 cgactccttc ggctcccttg atcgggctca ttcccggcgt gtcaagtctt gggcgacaca
     4381 gcatcggatg acggaaggcc gctccgaccc ccgactcccg agccgcgact cccgcaccag
     4441 atatcatccg cgccgtgacc acctacccac tcgaaccgga cgccgccgaa atgcaccgcc
     4501 tcgttgacag cgccatgcag cgcatcgtcg agcacatcga gtcgctgccg catcagcccg
     4561 cctcgaacgt ggagggggca accgatcgtg cccgcacgct catcgagcct ttgccgggca
     4621 gcgcgacgcc ctatgaccag ctcctcgact ttcttttcga cgaggccatc ccgcgctcgt
     4681 tcaacgccgc cggccccggc tatctcgcct tcatcccggg cggtgggatc tttcacgcag
     4741 cggtcgccga cttgatcgcc aatgcggtga atcgctacgt cggcgtctgc gccgcggctc
     4801 cactgctcgc ccaaatcgaa gcgaatgtcg ttcgctggtt ctgcgagatc atcggctacg
     4861 gcgagggcag cggcggcgtc ctcacaagcg gcggttcgct cgccaatctc accgccatca
     4921 tcgccgcccg ccgcgccctc ctcggcgacg atttccggca agcaacgctc tactgcggtg
     4981 atcagacgca tcacgccttt cagaaagccg cgatcctggc cggcttcccc gctgcgaacc
     5041 tccgcgagat tccgagcgac gcgaagtttc gcgttcgtgc agacttgctc gttgaaacca
     5101 tcgcccgcga ccgcgccgcc ggcctcacgc cgttcatgat ctgcggcagc gcgggaacga
     5161 cagcaacggg cgccgtcgat gacctggatg ccctgggcaa aatcgcgaag tcggagtcgc
     5221 tctggtttca cgtcgacggc gcgtacgggg cattcttcct gctgaccgag cgcggacgcg
     5281 cggccatgcg cggcatcgag caggccgact ccgtcattct cgatccgcac aaaacg
//
LOCUS       DATLVW010000598        18700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_395875, whole genome shotgun sequence.
ACCESSION   DATLVW010000598 DATLVW010000000
VERSION     DATLVW010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395875"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VNN25_17685"
     CDS             <1..339
                     /locus_tag="VNN25_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93420.1"
                     /translation="PDYFPYFNAFGGSDPSRYLVDSNIDWGQDILRLRSVARREHIEK
                     LAASLMGPADYVALGFPPVELASPWTESHGWVAVSDHSYQLSGAEGGWRWLPETYERV
                     GKSIRLYRIP"
     gene            476..2077
                     /locus_tag="VNN25_17690"
     CDS             476..2077
                     /locus_tag="VNN25_17690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH93421.1"
                     /translation="MQRFAFRPATLAALLIAIGAIRMATTFRVFSVTNDESAHVGVGM
                     ELLEYHRFNLQSENPPLAPLIFAFIPELGGMRFDPTGTFSNQIHSVFYGHGEYKANLF
                     RARAGTLVFFVIAAIALFFAARDALGDAGALAATFLFTMEPVVLGYSALATHDGPAVA
                     GLAVALLAFLHWLRRPDLTRALIFGAAFGFSINCKFSSIVFVPVTCVAIAAVRLFRDA
                     ELRHKFGRAIVTMLPAAAVTLLVIWAGYGFTVHTFAELQPWMDTYPHSVQHLLAHISP
                     STPLPAPEFFVGISAIHRTNQEGFQTFLCGQLSNTGWWWYFPFAVALKTTIAALVLFV
                     SGAWFALRDRALRWPFAEWSIAALATIVIAMPSTLDVGIRYILPFYVPFAIASAAAVL
                     AMLHASRASAAVAIALLVCHLGASALAHPDYFPYFNLFAGRDPSRYLVDSNLDWGQDI
                     LRLRSVVRREHIPSLTFALMGPADLRALGFPTAYPISMWTRSHGWIAISDHPRQVMQE
                     QGALRWLPPQPTRRVGKSISLYYIP"
     gene            2096..4372
                     /locus_tag="VNN25_17695"
     CDS             2096..4372
                     /locus_tag="VNN25_17695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH93422.1"
                     /translation="MQPLPKLRPAMLAALLILLGCVRIASTWRTFSDTVDEATHIGAG
                     LELLQYHQYELQPENPPLPRIVMAAAPLAGGMRWPVEGNRFQKIHSLVYGRGKYERNL
                     VLMRAGNLLFFILAGWALFVWIRREFGELEALIAILLFTMQPIILGYSGLATFDTAAT
                     AGLAVALIAFSAWLRKPDALHAALLGAGYGFSILMKFSSIGYVPAVCAAIYVVRLLGD
                     PERRRERLRALATVLIVPPVTLAVIWAGYAFTVGRLSELQHFRGEFGILDRVLLSHLP
                     QSMPLPAPSFFRGVGGLLEIGHADFDSYLFGHISKSGFWWYFPAAVGLKTTLAFFALL
                     FAGIVAAWTNHSRRWAMAEALAGSVAIFAVAMPSILDLGVRYVLPVYVPLTAAAAIGT
                     VALLDSARKEIRIAAMLLVGLHIVASLAAHPDYFPYFNALAGRDPGRDLIDSNLDWGQ
                     DLLRLRSTARTLRIDRMAVNLGYHDYEALGFPPQHEVVSTHPEKGWIAIGEHVYRLEH
                     AKGAWSWLDPFPMTRVGMSIRLYNIPPNAALDTETLGNEEILLPIAGTLGAVGGPAGT
                     WFRVDQTIRNTSTKRARVLVSSCGTKPAPCEITLDPGQTERLASGPGERPFILVTLPR
                     AAAKQLEFSTVVHAGNWPDVTIPEVAEDAFQKDRIVVPGVPTNARLNLRAWVRPVSGA
                     PIVVRLMSSRDQRVIGEKQFAADPTGYFAAGDLGHEFPQLHGESVDVTIESTGSQVWA
                     MMTTTDYQTGKIVLSLPR"
     gene            4423..6402
                     /locus_tag="VNN25_17700"
     CDS             4423..6402
                     /locus_tag="VNN25_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93423.1"
                     /translation="MHHTRLPKWEPIAIATVAVARLWLTAVGTLKAIGWASIDDYWFL
                     QRARSIMAGQWMGPYNHLTLIKGPGYPLWIAAISSLHIPLLFAQQLLYTLACLAVWRA
                     VAPSVRSAPARVALFAVLAFNPVSYSNDIADHVSREGVYPALSLLVCAGVAGILLRLV
                     ARRRDVLPWVILAGVAFAVLWHTREESIWIAPLPVFGAAGVLAWVLRDRQARWKRAVL
                     VLVPPALFLVAVHAAIVITNGIRYGVWSDVEFKWRPFVLAYSSLSAVRPHPPVPHTPA
                     PKEVRERVYAVSPAFAELRPLLEGKLGMDWRQGSADLENGMLMWAFREAVQNAGYYAR
                     GSAAVANFYDRVSREIDAARLSGRLDAREARASLLPPLLSGQRREIVRRWFFGAMRLA
                     RFADSHIAPTYSEGSDRELREFAETTRTPLTPRARSVGRVHLVAWIIHAEGSLDVTVE
                     HSDGTPVPGARVTRFPTPDLYDHLRTSWKDFPPARNAGFEIHAQEPDAQLVLSLRGRR
                     IDRIALSTDLPKPHDPLVRIGVYQYEKTVGEPDLSSIDTLRLAVLNAIGRFYQVTFPL
                     VLVVALLLFVLTRKWNAGQRGDWMIAILIGGIFAAIAARLMILSIIDVTSFGVFAVGY
                     ESPAYPLLLTGTFLAAHQGVAALRRRHHGGEPAST"
     gene            complement(6399..9023)
                     /gene="clpB"
                     /locus_tag="VNN25_17705"
     CDS             complement(6399..9023)
                     /gene="clpB"
                     /locus_tag="VNN25_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HXH93424.1"
                     /translation="MNFNKLTLKAQEAVAEAQNLSRGAGNPEVTAEHLLIALLRQEGG
                     IVGPILTKLGIQPGQIEAETAAEIAKYSRVAGASAEPITGPTLRKVFDAAFNAATQFN
                     DEYVSTEHFLLAIAQTKEATAAKILARHGAVPDALLKALQSVRGTQRVTDQNPEDKYQ
                     ALERYARDLTQLARRGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLARRVVEGDVPEGLKNKRVVALDLGAMIAGAKYRGEFEDRLKAVIKEVEESEGNVVL
                     FIDELHTLIGAGASEGAMDASNMLKPALARGELRAIGATTLNEYQKHIEKDAALARRF
                     QPVFVGEPTVEDTIAILRGLKERYEVHHGVKIQDSAIVAAAMLSHRYITDRFLPDKAI
                     DLVDEAASRLRIELTSMPQEIDELTRRMTQLQIEKQALSRETNPASKQRLGEIESEIG
                     GLQEKISTLRTQWENEKGVITQITDLKKKIEETREAANAAERQGDLNKAAELRYGALP
                     QFNRDLEAASARLMEMQKGGALLREEVTDEDIAEIVSKWTGVPVTRMMESEIQKLVRM
                     EDNLKLRVIGQEEAITAVSNAVRRSRAGLKDPSRPIGSFIFMGPTGVGKTELARALAE
                     FLFDDENAMVRIDMSEYMERHAVARLIGAPPGYVGYEEGGQLTEAIRRRPYAVLLFDE
                     IEKAHPEVFNIMLQILDDGRLTDSKGRVVDFKNTVIIMTSNVGAQFIFDYQKSMQTPA
                     QEAEMERKVQEELHRHFRPEFLNRIDDVIIFHALDMQHIKRIIEIQIRKLTKMIADRG
                     ITIEISDHAKEQLAKEGYDPAFGARPLKRALQKLIIDPLAMKLLEGKFKPGDIVFVNV
                     GDDGKIELSLEPEPVAVN"
     gene            9378..9602
                     /locus_tag="VNN25_17710"
     CDS             9378..9602
                     /locus_tag="VNN25_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93425.1"
                     /translation="MSKTLKAEYDDAARSLRLLEPLDGFADREKVTVIVEHADTKRPW
                     SDFRGLLSGKEGEEFARVIEEAFPIEPVKK"
     gene            9624..10487
                     /locus_tag="VNN25_17715"
     CDS             9624..10487
                     /locus_tag="VNN25_17715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH93426.1"
                     /translation="MTLPRITVVTPSLDQAPFLEQTILSVLGQDYPNLEYIIVDGGST
                     DGSVEIIRMYEHQLAWWLSEKDDGQAAAINRGFARATGDILAWLNSDDMYLPGTLPYI
                     GGVLNPGRAEVRFGNCTHLVEKQRVTRGSDVRRMHNEMDLALADYIIQPSAFWTRKSW
                     TETGPLDESLNFALDWDWFLRARKKGVAFVPDDRYLAVYRMHAAHKSANGGERRQREL
                     ATIYDSHAGAKYEQLFLQCCARRDAIQSIRAWLGRARLPRLVPPLLRAAYPSVFGSFP
                     DNEIRDVLTML"
     gene            10519..12570
                     /locus_tag="VNN25_17720"
     CDS             10519..12570
                     /locus_tag="VNN25_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93427.1"
                     /translation="MNGTSRRDLFLVTFSILSLELAVIRWMSQQVTLFAYLGSVLLIG
                     AFLGMGLGVALGRKRPALFGLTLPALAALSGVLAFSEKLNILHTRFPGRSVAMWGMEN
                     AKNFASSMAVIVALFAAVVIVFLLAGTRVGEIFAASEALDAYAVDLTGSFAGVVAMAI
                     VSSAQTPPPVWFALGGIPLAFVSRRWTSWIALGAVLLFTTLSIRGAIFSPYYRIDLDR
                     AGDVTGAPIRLSVNRDFHQYIHDFSPRHLNDPALSPDVRMRLASAEATYRLPFLLAPR
                     RDSALVVGAGTGNDVAAALRQGFREVVSVDIDPRIIDIGRDLHPERPYADPRTIPVVN
                     DARAFFEQTNRQFDVVDFGLLDSHAVFSAMSSLRLDNYVYTVEAMRSAWRHVRTPGLM
                     SVSFSIAEREWLSDRLDKIVHDATGVDPVIVPHAIQRGRFFIVTKGIDPRVLAANFGL
                     RTLPAPDTTHVEAATDDWPFLYLSPGEIPVGYIAMVAAILLIALIGTRAVFGPGLFRG
                     QSFDPMLFVMGAAFLLIETRSVTDLSLLFGSTWVVNAAVFAGVLAVVFTANALVRKGR
                     TPNILLVFGLLFASLLVNYFVRPDQLLTLGLATRSILGPLLAALPVGFAGIIFSSVFA
                     RSAEPNASLGSNLLGAVVGGCLEFLSMATGLRALTLLALAFYLVALFLYRRSEATIAS
                     A"
     gene            12627..13487
                     /locus_tag="VNN25_17725"
                     /pseudo
     CDS             12627..13487
                     /locus_tag="VNN25_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            13598..14314
                     /locus_tag="VNN25_17730"
     CDS             13598..14314
                     /locus_tag="VNN25_17730"
                     /inference="COORDINATES: protein motif:HMM:NF025716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HXH93428.1"
                     /translation="MRINGRFLVACLDAMLLVAFVLLVSVHLTGLRVHEWLGIAFVLL
                     VLVHLLYSWRWILTHTRRIFSGGSRRARINYLLNVTLFIIVMIVVISGLVISQAALPS
                     LGIATIDDGAWRTLHNKSQTWALVAVGVHIAMNWTWIRAALRRGWVVSNRLSRGPQLA
                     PSLWHVLRRSAVIAIATCVIAGGLWLIVGLPSRQRELKLNEMARWNVQPGPGIGQMLT
                     RGALLAIAGLAARRWLRVHI"
     gene            14374..14943
                     /locus_tag="VNN25_17735"
                     /pseudo
     CDS             14374..14943
                     /locus_tag="VNN25_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
     gene            15001..17472
                     /locus_tag="VNN25_17740"
     CDS             15001..17472
                     /locus_tag="VNN25_17740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH93429.1"
                     /translation="MKHAAIEPSTRVWAAPDFQSIAFGREGEILAAKVRLGIMALIGL
                     IPLQTVLFRAANAEAKIGLGVTILVLVMGTVVLRVAQRPKPPAWLGLFTCLLDVSTVS
                     MVNAGFLLSGNPLAVTNSRVVFCCYFVALALVCLRQDWRWCLIAATVAMMEYGCIVAW
                     AAARYDLYGAAFATSGYGRFNWDNQIARLMLLAAAGVIGAVTVVQSRGYWEAVIKYLD
                     AFPLGVIIAGADHRSRYANRAAQELLNRDIPPGVQLSELNGQLFLSGTDDPYPPRQTT
                     IALALAGESTECDDIEIQRPGSRIAVEAWGTPVLDSKGRVSNAIAVFHDETRRRRAEE
                     EMRRLGEMLREANAELALRGVILESVAVAAERLFQSASWEEVATEVIGGIGSAVGASR
                     ASIFQVSDDGELAARLRFGWVAPGVVPIGEAMRQKVPFRVSGFGRWLDSFSRGETVHG
                     PVRDFPESEQTVLTPQGVRSLAVVPVHLGERLWGFLGFDDCVSDRQWSQGEILGLRVA
                     ADMLAEAIAAKERAAAIEASEERYRTLFERSLGLLCTHSMDGTILTVNHAAASRLGLT
                     IDQMIGRNLEHFLAPSALAKFPEYLAAIAATGQASGNLVLSDTNGTERVWEYRNYVVR
                     EPNQEAYVLGHALDVTDRTTLERELRDRALKDPLTGMANRTLFEDRLTRAFERAKRRA
                     IAEEETQPWALAYLDLDGFKETNDRFGHPAGDALLCEVATRLRHGLRALDTVARLGGD
                     EFALILPDIRSQENAKHVIDKLMETLRYPFLYSGYTLVVSVSVGVSMHPLDGESTEAL
                     TARADKAMYAAKSAGGSGYRFYSDL"
     gene            17475..>18700
                     /locus_tag="VNN25_17745"
     CDS             17475..>18700
                     /locus_tag="VNN25_17745"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HXH93430.1"
                     /translation="MRTERDTLGEVQVPDDALYGAQTQRAVENYPISGLRAHPVFIRS
                     FILLKRAAALANRELGTLDAALSDAIARACDRLLGDDEGNRKHFPVDVFQAGAGTSFN
                     MNCNEVIANLANVSLSGHLGEYKPIHPNDHVNMAQSTNDVFPTAIRIATLLLLRELTT
                     QLDALAAAFDDRGNAFADVIKSGRTHLQDAVPLTLGQEFRAYASATRRATRLLTASAA
                     ELRILGIGGTAVGTGMNAPRGYRQVVVRNLNALTNLDLVPTDDLREAMQSQLPVSAVS
                     GALRNLALELTRITNDLRLLASGPQTGLAEITLPSVQPGSSIMPGKVNPSMLECMNQV
                     LFHIIGSATTIDYAVQAGQLELNVMMPLMAFEILFSIEILKNYLPVFIEKCIRDIEAD
                     RAQCEAYYISSPSLATA"
BASE COUNT         3168 a         6168 c         5600 g         3764 t
ORIGIN      
        1 cccgactact tcccatactt caacgccttc ggcggctccg acccgtcgcg ctacctcgtc
       61 gacagcaaca tcgactgggg ccaggacatc ctccgccttc gcagcgtcgc gcggcgcgag
      121 cacatcgaaa agctcgctgc atcgctgatg ggtccggccg attacgtggc cctcggcttt
      181 ccgcccgtcg agcttgccag tccttggacg gaatcacacg gatgggtagc ggtcagcgac
      241 cactcgtacc aactctcggg cgccgagggt gggtggcggt ggctgccgga aacgtatgag
      301 cgggtgggaa agtcgattcg gttgtatcgg attccgtagc agccttccac cacggctaag
      361 actttctcac gcgaagacgc gaaggttgga tgtttgcgat gcgatccttc gcgaccttcg
      421 cggcttcgcg tgagaaatgg ggttcagtgg gcgcgcagta gaatccccgc ccaaaatgca
      481 gcgcttcgca tttcgtcccg ccactcttgc cgcgttgctc atcgcgatcg gtgccatccg
      541 catggccacc acgttccgcg tcttttcagt aaccaacgac gagtcggccc acgtcggcgt
      601 gggaatggaa ctgctcgagt atcaccgctt caacctgcag agcgagaacc cgccgctcgc
      661 accgctcatc ttcgccttca ttcccgagct gggcggaatg cgtttcgatc cgacgggcac
      721 gttcagtaat cagattcaca gcgtcttcta cggacacggc gagtacaaag ccaatctctt
      781 tcgcgcgcgc gccggcacgc tcgtcttctt cgtcattgca gccatcgcgc ttttcttcgc
      841 cgcgcgcgat gcgctcggtg acgccggcgc gctcgcggcc accttcctct tcacgatgga
      901 gccggtcgtc ctcggctact cggcgctggc tacgcacgac ggccctgcag tagccggtct
      961 ggccgtcgcc cttctggcgt ttctgcattg gctgcgccgc cccgatctga cgcgcgcact
     1021 catctttggc gccgcgttcg gcttcagcat caactgcaaa ttctccagca tcgtcttcgt
     1081 gccggtgacc tgcgttgcca tcgcggccgt ccgcttgttt cgcgatgcag agctacggca
     1141 caagttcgga cgcgccatcg tcacgatgct gcccgcagcg gccgtgacac tcctcgtgat
     1201 ctgggcaggc tacggattca cggtgcacac attcgcggag ctgcagccgt ggatggatac
     1261 gtacccgcac tcggtgcagc atcttctcgc gcacatcagc ccctccacgc cgctgcctgc
     1321 gcctgagttt ttcgtcggca tcagcgccat tcaccgaacg aaccaggagg gattccaaac
     1381 ctttctttgc ggacaactca gcaacaccgg atggtggtgg tatttcccgt ttgctgtcgc
     1441 gttgaagacg acgatcgccg cgctcgtgct cttcgtctcc ggcgcctggt ttgcgctgcg
     1501 cgaccgtgcc ttgcgctggc ccttcgcgga atggagcatc gccgctctcg ccacgatcgt
     1561 catcgcgatg ccgagcacgc tcgacgtcgg catccgctac atcctcccgt tctacgtgcc
     1621 attcgccatt gcctcggccg cggccgtact ggcgatgctg cacgcctcca gagcgagcgc
     1681 cgccgtcgcg atcgcgcttc tcgtctgcca cctcggcgca tccgcgctcg cacatcccga
     1741 ctacttcccc tacttcaacc tcttcgctgg gcgcgacccc tcgcgctatc tcgtcgacag
     1801 caacctcgat tggggacagg acatcctccg cctccgttcc gtcgtccggc gcgaacacat
     1861 cccgtcgctc accttcgcgc tcatgggtcc cgccgatctg cgggctctcg gatttccaac
     1921 ggcctacccg atcagcatgt ggacccgctc tcacggatgg atcgcgatca gcgaccatcc
     1981 ccgccaggtg atgcaggagc agggcgcgct caggtggctg ccgccgcaac ccacgcgccg
     2041 tgtgggcaaa tcaatctcgc tctactacat cccctgaacg gctatattcg cgccgatgca
     2101 gcccttgccg aaactccggc ctgcaatgct ggccgcgctg ctgatcctcc tcggctgtgt
     2161 acgcatcgcc tcgacctgga gaacgttctc cgacacggtc gatgaggcca cgcacatcgg
     2221 cgccggtctc gagctcctgc aataccacca atacgaacta cagccggaga acccgccgct
     2281 tccgcgcatc gtgatggcgg cagcaccgct ggcgggcggc atgcgatggc cggtcgaggg
     2341 aaaccggttc cagaagattc atagcctcgt gtatgggcgc ggcaagtacg agcggaatct
     2401 cgtgctcatg cgcgccggca atctgctttt tttcatcctc gcgggctggg ctctcttcgt
     2461 ctggatacgg cgcgaattcg gcgaactcga ggcgctcatc gccatcctgc tcttcacaat
     2521 gcagccgatc atcctcggct actccggact ggccacgttc gacaccgccg ccaccgccgg
     2581 actggccgtc gcgctgatcg ctttctcagc gtggctgcgc aagccggacg ccctgcacgc
     2641 cgcgctgctc ggcgccggct acggcttcag catcctgatg aagttctctt cgatcggcta
     2701 cgtccccgcg gtctgcgccg caatttatgt cgtgcgtttg ctcggcgacc cggagcggcg
     2761 gcgcgagcgc ctgcgcgcgt tggctacggt tctcatcgtt ccgcctgtga cgttggccgt
     2821 catctgggcg ggatacgcct tcaccgtcgg ccggttgtcg gagcttcaac attttcgcgg
     2881 cgagttcggc atcctcgacc gcgttctgct gtcgcatctg ccgcaatcaa tgccgttgcc
     2941 tgcgccttca tttttccgcg gcgtcggcgg tctgctcgaa atcggtcacg ccgatttcga
     3001 ctcctacctc ttcggccaca tcagcaagag cggattctgg tggtacttcc ctgccgctgt
     3061 tggactgaag acgacgcttg cgtttttcgc gttgctgttc gcgggcatcg tggcagcgtg
     3121 gacgaatcat tcgcgacgat gggcgatggc cgaggcgctt gcgggatcgg tggcgatatt
     3181 cgcggtggcc atgcccagca ttctcgacct cggcgtgcgt tacgtcctgc cggtctacgt
     3241 gccgctgacc gccgcagccg cgattggaac ggttgcgctg ctcgacagcg cgcgcaaaga
     3301 gattcgcatc gccgcgatgt tgcttgttgg cctgcacatc gtcgcatcgc tcgccgcaca
     3361 tcccgactat ttcccctatt tcaacgcgct ggcgggacgc gatcccggac gcgatctcat
     3421 cgacagcaat ctcgactggg gccaggatct gcttcgactt cgcagtaccg cgcgaacact
     3481 ccgcatcgac cggatggccg tcaacctcgg ctaccacgac tacgaagcgc tcggatttcc
     3541 accccagcac gaagtcgtat ccacgcatcc cgaaaagggt tggatcgcca tcggagagca
     3601 cgtctatcgc ctcgagcacg cgaagggcgc atggtcgtgg ctcgacccgt ttccgatgac
     3661 gcgcgtcggc atgtcgatcc gcctttacaa catccctccc aacgccgcgc tcgacacaga
     3721 aacgctgggc aacgaagaga tccttcttcc gatcgccggc accctcggcg ccgtgggcgg
     3781 tcctgccggc acctggttcc gcgtcgatca gaccatccgc aacaccagca caaagcgcgc
     3841 tcgcgttctc gtcagttcgt gcggaacgaa gccggcgccg tgcgagatca cgctcgatcc
     3901 cggccagacc gagcgcctcg cctcggggcc aggagagcgg ccgttcatcc tcgtgacgct
     3961 gcctcgcgct gcggcgaagc aactggaatt ctcgacagtc gttcacgccg gaaactggcc
     4021 tgatgtcact atccccgagg tcgccgaaga cgcctttcag aaggaccgca tcgtggttcc
     4081 gggcgtaccg acgaacgccc gcttgaacct gcgcgcctgg gtccgtcccg tttccggcgc
     4141 gccgatcgtc gtccggctca tgtcatcacg agaccagcgg gtgatcggcg agaaacagtt
     4201 cgccgccgac ccgaccggct acttcgcggc cggcgacctc ggccacgagt ttccgcaact
     4261 ccatggcgag tcggtcgacg tgacgatcga atcgaccggc tcgcaggtgt gggcgatgat
     4321 gacgaccacc gattatcaaa ccggcaagat cgtgctctcg ctgccgcgat gagggtgcgt
     4381 tagccttcat ttctcacacc tgcgcgcgtc tatcattggg cgatgcacca tacgaggctc
     4441 ccaaaatggg aaccgatcgc gatcgccacg gtcgccgttg cgcgcctttg gctgacagcg
     4501 gtgggaactc tcaaggcgat cggctgggcg tcgatcgacg actactggtt tctgcaacgg
     4561 gcccgctcga tcatggcagg tcagtggatg gggccgtaca accatctgac gctgatcaaa
     4621 ggcccagggt atccgctctg gatcgcggcg atctcgtcgc ttcacattcc tttgctgttc
     4681 gcgcagcaat tgctgtacac gcttgcgtgt ctggcggtct ggcgcgcagt tgcaccatca
     4741 gttcgctcgg cgcccgcgcg cgtcgccctg tttgccgtgc tcgcgttcaa tccggtgagc
     4801 tacagcaacg acatcgccga tcacgtctcg cgtgagggcg tctatcctgc actttctctg
     4861 ctggtctgcg caggtgtggc aggcatcctc ctgcggctcg tcgctcgccg gcgcgacgtg
     4921 cttccctggg tcatcctcgc tggcgtcgcc tttgcggtgc tctggcacac tcgcgaagaa
     4981 agcatctgga tcgctccgct tcccgtgttt ggagccgccg gagtcctggc atgggttctc
     5041 agggaccggc aggcacgctg gaaacgcgcc gtgttggtgc tggtcccgcc cgcgctcttc
     5101 ctggtagcgg tgcatgcggc catcgtcatc accaatggca tccgatacgg cgtctggtcc
     5161 gacgtcgaat tcaaatggcg tccgttcgtc ctcgcctaca gcagcctcag tgccgtgcgc
     5221 ccgcatcctc cggtgccgca tacaccagcg ccaaaagagg ttcgggagcg cgtctacgcc
     5281 gtgagccccg cattcgcaga gctccgtccg ctgctcgagg gaaagctcgg aatggactgg
     5341 aggcagggca gcgcggacct cgagaatgga atgctcatgt gggcgtttcg tgaggccgtg
     5401 cagaacgcgg gctactacgc gcgagggtca gccgcggtcg ccaatttcta cgaccgcgtc
     5461 agccgcgaga tcgacgcggc gcgcctctcc ggacgcctcg atgcgcggga agcacgcgcc
     5521 tctcttctcc ctccactact gtccggtcag cggcgagaaa ttgtccgtcg atggttcttt
     5581 ggcgccatgc ggcttgccag gttcgccgat tcccatatcg caccgacgta ttcggaaggg
     5641 tcggatcgcg aactgcgcga gttcgcggag acgactcgaa cgccgctgac accgagagca
     5701 agaagcgtcg gccgggtgca tctcgtcgca tggatcattc acgcggaggg ttcgctcgac
     5761 gtcacggtcg aacattccga cggaacgccg gttcccggcg cgcgcgtgac gcgtttcccg
     5821 acgcccgacc tgtacgacca tctaagaaca agctggaagg atttcccccc ggcgcggaac
     5881 gcaggcttcg agattcatgc gcaggagccg gacgcgcaac tcgtgctgtc attgcgcggg
     5941 cgacggatcg accgcatcgc gctcagcacg gatttgccca agccacacga cccgctcgtg
     6001 cgcatagggg tttatcaata cgagaagacc gtgggcgagc cagacctgag ttcgatcgac
     6061 acccttcggc tcgccgttct gaacgcaatc ggccgtttct atcaggtcac ctttccgctg
     6121 gtactcgtag tcgcgcttct gctctttgtc ctgacgcgga aatggaacgc cggacagcga
     6181 ggcgattgga tgattgcgat cctcatcggc gggatcttcg cagcaatcgc agcacgattg
     6241 atgatcctct ccatcattga tgtaacgtcg ttcggggtct tcgccgttgg ctacgagtca
     6301 cccgcgtatc cactactgct gaccgggacc tttcttgcgg cgcaccaagg cgtggctgcg
     6361 ttgcgccggc gacaccatgg tggtgagccg gcgtcaactt agttcaccgc gaccggctcc
     6421 ggttcgagcg acagctcgat ctttccatcg tcacccacgt tgacgaagac gatgtccccc
     6481 ggcttgaact ttccttcgag cagtttcatc gccagcggat cgatgatcag tttctgcaac
     6541 gcgcgcttga gcggacgcgc gccgaaggcc gggtcgtaac cctctttcgc gagctgctct
     6601 ttggcgtggt cgctgatctc gatggtgatg ccgcggtcgg cgatcatctt ggtcagcttg
     6661 cggatctgga tctcgatgat ccgcttgatg tgctgcatgt cgagcgcgtg gaaaatgatc
     6721 acgtcgtcga tgcggttgag aaactcgggg cggaagtggc ggtgcagttc ctcctgaacc
     6781 tttcgctcca tctccgcttc ctgcgccggc gtctgcatcg atttctgata gtcgaagatg
     6841 aactgcgcgc cgacgttcga agtcatgatg atgaccgtgt tcttgaagtc gaccacgcgg
     6901 cccttcgaat cggtgaggcg tccgtcatcc agaatctgca acatgatgtt gaagacctcg
     6961 ggatgcgctt tctcgatctc gtcgaacagc agcacggcat acggacgtct ccggatcgcc
     7021 tcggtgagct ggccaccttc ctcgtatccg acgtagccgg gcggcgcgcc gatcagccgc
     7081 gccacggcgt ggcgctccat gtactccgac atgtcgatgc gcaccatggc gttctcgtcg
     7141 tcgaatagaa actcagccag cgcacgcgcc agctccgtct tgccgacgcc ggtcggtccc
     7201 atgaagatga atgagccgat cgggcggctg ggatccttga gccccgcgcg cgagcgccgc
     7261 acggcgttgg agacggcagt gatcgcctcc tcctgaccga tgacgcggag cttgaggttg
     7321 tcctccatcc gcacgagctt ctgaatctcc gactccatca ttcgcgtgac cggtacgccg
     7381 gtccacttcg agacgatctc ggcgatgtct tcgtcagtca cttcctcccg gagaagcgcg
     7441 ccgcccttct gcatctccat gaggcgcgcc gaggcggctt cgagatcgcg attgaactgc
     7501 ggcagcgcgc cgtagcggag ctcggccgcc ttgttcaggt cgccctgacg ctccgcggca
     7561 ttggcagcct cgcgcgtctc ttcgatcttc tttttgagat cagtgatctg cgtgatgacg
     7621 cccttttcgt tttcccactg cgtgcgcagc gtgctgatct tttcctgcag cccgccgatc
     7681 tcgctctcga tctcgccgag gcgttgtttc gaagcgggat tggtctcgcg ggagagcgcc
     7741 tgcttttcga tctggagctg cgtcatgcgt cgcgtcagct cgtcaatctc ctgcggcatc
     7801 gatgtcaact cgatccgcag acgcgacgcc gcttcgtcga caaggtcgat cgccttgtcg
     7861 ggcaggaacc ggtcggtgat gtagcggtgc gacaacatcg ccgcagcgac gatcgcggag
     7921 tcctgaatct tcacaccgtg atgcacttcg tagcgttctt tgagtccgcg aagaatggcg
     7981 atcgtgtctt cgacggtcgg ctcgccgacg aagaccggct ggaagcggcg ggcgagcgcg
     8041 gcgtcctttt cgatgtgctt ctgatattcg ttgagagtgg tggcgccaat cgcgcgcagc
     8101 tcgcctcgcg ccagcgcggg cttgagcatg ttcgaggcgt ccattgcccc ttccgaggcg
     8161 ccggcgccga tgagcgtgtg gagctcatcg atgaaaagaa cgacgtttcc ttcgctctcc
     8221 tcgacttcct tgatgacggc cttgaggcgg tcttcgaatt cgccgcggta tttcgcgccg
     8281 gcgatcatcg cgccgaggtc aagcgcgaca acacgcttgt tcttgagacc ttccggaacg
     8341 tcgccttcga ccacgcgccg cgccagcccc tcgacgatgg cggtcttgcc gacacccggc
     8401 tcgccgatca ggacgggatt gttcttcgtt ctgcgcgaga gcacctgaat cacgcggcga
     8461 atctcttcat cgcggccgat gacgggatcg agtttgccgc ggcgcgcgag ctgggtgagg
     8521 tcgcgcgcgt agcgctcgag agcctggtac ttgtcctccg gattctggtc ggtcacgcgc
     8581 tgggttccgc gaaccgactg cagcgctttg agcagcgcgt cgggcacggc accgtgccgc
     8641 gcgaggatct ttgctgcggt cgcctctttc gtctgtgcga tcgcaagcag gaagtgttcg
     8701 gtcgaaacgt actcgtcgtt gaactgggtg gcggcgttga atgcggcgtc gaagaccttc
     8761 cgaagagtcg gaccggtgat cggctccgcc gaggcgccgg cgacgcggga gtatttggcg
     8821 atctctgcgg cggtctcggc ctcgatctgc cccggctgaa tcccgagttt ggtcaggatc
     8881 ggcccgacga ttccgccctc ctgccgcagg agtgcgatca atagatgttc ggcggtaact
     8941 tccgggttgc ccgcccctcg ggaaaggttc tgcgcctcag ccacggcttc ctgggccttg
     9001 agtgtaagtt tgttgaaatt catatgatta cctcacattg agtctcgcgg actcacatcg
     9061 caaaaccggc aaaagttacc ggacattccg gcaagatcgc ctctcgtcgg ggagtgcaag
     9121 aagggagcgc gaccgcgttc ttcgtcctcg cgtaaactac gagctggtat ggacaaggac
     9181 gacaaaaaag gcatcggccc ggcgcgtccg tggcgagatt tggagaacat actctccgga
     9241 gaagctggcg cgtcgttcgc gcgagcgatc gaagaagcgt ttcccatcga gcctgtcaag
     9301 agcggatcat cctcatcgcc gttgactatt gatgaaacgt ttcacgcgga gacgcggagg
     9361 gcgcggagtt gaggtcgatg agcaagaccc tcaaagctga atacgacgac gcagcacgta
     9421 gccttcgttt gctcgagccg ctggatggtt tcgccgaccg cgagaaggtt accgtcatcg
     9481 ttgaacatgc cgatacgaag aggccttgga gtgactttcg cggattgctc tcgggcaagg
     9541 aaggcgaaga gttcgcgcga gtgatcgaag aagcatttcc aatcgagccc gtgaagaagt
     9601 gatcgatcgt cagtgctcgc gcgatgactc ttccacgaat caccgtcgtc accccctcgc
     9661 tagatcaggc accctttctc gagcagacca tcctctccgt gctcgggcag gactacccca
     9721 acctcgaata catcatcgtc gacggcggca gcacggacgg cagcgtcgag atcatcagga
     9781 tgtacgaaca ccaactggct tggtggttga gcgaaaaaga cgatgggcag gccgcggcca
     9841 tcaatcgcgg tttcgcgcgg gccacgggcg acatcctcgc gtggctgaac tcggatgaca
     9901 tgtacctgcc gggtacgctg ccttacatcg gcggcgttct caatcccggg cgcgccgagg
     9961 ttcgctttgg caactgcacg catctcgttg agaagcagcg agtaactcgc gggagcgacg
    10021 tcaggcgcat gcataacgaa atggacctcg cactcgccga ctacatcatt cagccgagcg
    10081 cgttctggac gcggaagtcg tggaccgaga ccggtccgct cgatgagtcc ctcaacttcg
    10141 cgctcgactg ggattggttc cttcgcgccc ggaagaaggg cgttgctttc gttcccgacg
    10201 atcgctacct cgccgtctac cggatgcacg cggcgcacaa aagcgcgaac ggcggcgaac
    10261 ggcgacagag agaactagcc acgatctatg attcgcacgc cggcgcgaag tacgagcagt
    10321 tgttcctgca gtgctgcgcg cggcgcgatg ccatccaatc gattcgcgct tggcttggcc
    10381 gcgcccgatt gccgcgtctc gttccgccac ttctgcgagc ggcttaccca tccgtgtttg
    10441 gaagtttccc tgacaacgag atcagggacg tgctcacgat gctctagccg aagctccggc
    10501 cgctgttaga ctcccgccgt gaacgggaca agccgccgcg atctctttct cgtcacgttc
    10561 tcgattctct cgctcgagct cgccgtcatc cgctggatgt cgcaacaggt aacgctcttc
    10621 gcctacctcg gcagcgttct tctgatcggc gccttcctgg gcatgggtct gggcgttgcc
    10681 ctcgggcgaa aacggccggc gttgttcggc ctcaccctcc ctgctttggc ggccttgtcg
    10741 ggcgtgctgg cattctcgga gaagctgaac atccttcaca cccgctttcc cggacgcagt
    10801 gtggccatgt gggggatgga aaacgcgaag aactttgcat cgagcatggc ggtgatcgtc
    10861 gcgttgttcg ctgcggtcgt catcgtcttt ctcctggccg gcacacgggt cggcgaaatc
    10921 ttcgcagcct cggaagcgct cgatgcgtac gcagtcgatc tgaccggctc gttcgcagga
    10981 gtcgtggcca tggccatcgt ctcttcggcc cagacgcccc caccggtctg gttcgcgctg
    11041 gggggcattc cgctggcgtt cgtatcgcgc cgctggacca gctggattgc gctcggcgca
    11101 gtccttctct tcacgacgtt atcgatccgc ggcgcgatct tctcgccgta ctaccgcatc
    11161 gatctcgaca gagctggcga tgtaaccggc gctccgatcc gtctgtcggt gaaccgcgac
    11221 tttcaccaat acatccacga cttctccccg cgtcacctca acgatcctgc gctgagtcct
    11281 gacgtgcgga tgcgcctagc ctccgcggag gctacctatc gtctcccctt tctgctcgca
    11341 ccgcgccggg acagcgcgct cgtcgtcggc gccggcactg ggaatgacgt cgccgccgca
    11401 ctgcgtcagg gcttccgcga agtcgtctcc gtcgacatcg atcctcgcat catcgacatc
    11461 gggcgggacc ttcatcccga gcggccgtat gccgatcctc gcaccattcc ggtcgtgaac
    11521 gacgcccgtg cgttctttga acaaacgaac cggcagttcg atgtcgtcga cttcggtctt
    11581 ctcgattccc acgccgtctt ttccgcgatg tcgtcgctac ggctcgacaa ctacgtttac
    11641 acggtggaag cgatgcgctc ggcgtggcgc catgtgcgca cgccgggatt gatgagcgtc
    11701 agcttctcca ttgccgagcg cgaatggctc tcggaccgtc tcgacaagat cgtgcacgat
    11761 gccaccggcg tcgatccggt gatcgtccct catgcgatcc agcgcggccg cttcttcatc
    11821 gtcaccaaag gaatcgatcc gcgcgtgctg gcggcgaatt tcggactgcg caccctcccc
    11881 gcgccggata cgacccacgt cgaagcggca accgacgact ggccctttct ctacctgagc
    11941 cccggagaaa tccccgtcgg ctacatcgcc atggtcgcgg ccatcctgct gatcgcgctg
    12001 atcgggacgc gcgctgtgtt cggccccgga ctgtttcgcg gccagtcgtt cgatcccatg
    12061 ttgttcgtca tgggcgcggc gtttctcctg atcgaaacgc gtagcgttac cgacctttcg
    12121 ttgctctttg gatcgacatg ggtggtcaat gcggcagtct tcgcaggcgt ccttgccgtc
    12181 gtattcactg ccaacgccct cgtccgaaaa ggacgcacgc caaacatcct gctcgtgttc
    12241 ggccttctct tcgcatcgtt gctggtcaac tatttcgttc gcccggacca acttctcaca
    12301 cttggtctcg caacgcgctc aatcctcggg ccgcttctcg ccgcccttcc ggtcggattc
    12361 gccggcatca tcttttcgtc cgtctttgcc cgatccgccg agccgaacgc ctcgctcggc
    12421 tcgaacctgc tcggcgcggt cgtaggggga tgcctcgagt tcctctcgat ggccaccggc
    12481 ctgcgggcgc tcactcttct tgcgctggcc ttctacctcg tcgcactgtt tctctatcga
    12541 cgctccgagg ccacgatcgc gagcgcttga atgcgctttc taccggctga cccgcggaac
    12601 gactaaactt gccgtgagaa acccttatga cagcaacctc cccacttcgt gaagtacctg
    12661 cgcaagacac tctcttcgaa tccatcctcg aactgatccg gcggacctcg acgcaactgc
    12721 ctgacgacgt cgttcgtgcc gtcaccgggg cacggcagaa tgaagtcgaa ggatcgaacg
    12781 ccgacgtcgc tctgaacgtc atcggctgca acatcggcct ggcgcgcgac agctccctgc
    12841 cgctctgcca ggacaccggc accatcctct tctacgtcca cacacccgcc ggctacgacc
    12901 agcttgcgtt cgaagaggtt gcgacggaag ccgttcgcga ggctacgaag aaaggctacc
    12961 tgcggcagaa cagcgtcgac agcatcaccg gcaagaacac gggcgataac accggcccgg
    13021 gctcgccggt ctttcacttc caccagtggc gcgagcccca cgtagaggtg aagctgatgc
    13081 tcaaaggcgg cggctgcgag aatatgaacg cccaatactc gctcccccac cccgacctcg
    13141 gtaaccgcga tctcaaaggc gtcgagaacg ccatcctcga ctgcgttcta caggctcagg
    13201 gccagggctg cggaccaggc atcctcggcg tctgcatcgg cggcgaccgc ggcaccggct
    13261 acggctttgc gaaggagcag cttctccgcg tgctcgacga caccaatcca gatcccgtgc
    13321 tcgccgagat cgaggagcgg gtcgtcgaga aagcgaatca gtccggcatc ggaccgatgg
    13381 gcttcggcgg caaaaccaca ctcctcgcct gcaagatcgg caaactcaac cggctgccgg
    13441 cgtcgttctt cgtctcgatc gcctacatgt gctgggcata tcgccgtcgc ggcgcgcaac
    13501 tcgacctcgg cggaaacgtg acggagtggt tgtactgagc ctttccctct gacgtcatag
    13561 aactccgctg ctcatctctc gtattgttgt ttctgtcttg aggatcaacg gacgttttct
    13621 cgtggcctgc ctcgatgcga tgcttcttgt cgcgttcgtg ctcctcgttt cagtccatct
    13681 gaccggcctg cgcgtacacg aatggctcgg catcgcgttc gtgttgctcg tgctcgtcca
    13741 ccttctgtat tcgtggcgtt ggatcctgac gcatacacga cggatcttca gcggcggcag
    13801 ccggcgagcg cgcatcaatt acctgctcaa cgtaacgctg tttatcatcg tgatgatcgt
    13861 cgtcatctcc gggctcgtca tctcacaggc cgcgcttccg agcctcggta ttgcgacgat
    13921 cgacgatggg gcatggcgca cgctgcacaa taaatcgcag acttgggcgc tcgtcgctgt
    13981 gggcgttcac attgccatga attggacatg gattcgtgcc gcgctgcgcc gcggatgggt
    14041 ggtctcgaac cgtctgtctc gcgggccgca actcgcgcca tcgctttggc atgtgttgcg
    14101 ccgaagcgct gtcatcgcga ttgcaacgtg cgtaatcgcg ggtggcctct ggctgatcgt
    14161 aggattgccc tcgcgacagc gtgagctcaa gctgaatgaa atggctcgct ggaacgtgca
    14221 gccagggccg ggaatcggtc agatgctcac gcgaggcgct cttctcgcga tcgccgggct
    14281 tgctgcccgc cgctggctcc gcgtgcacat ctagtggtgt cattgggcac ccgcgacgag
    14341 cgcgctctcg tgacaaaatg cgtcgtcatg gctctcgtaa aactcactac tcccatcgac
    14401 gagcaaacca tccgttcgct tcacgtcggc gacaccgtca acatcaccgg ccgtctctac
    14461 actggccgtg acgcagtcca ccatcgcatc cataccggca caaagccgcc ggtcgatctc
    14521 gaaggccaga tcatctatca ctgcggtccg gtgatggtga aagagggcga tcagtgggtc
    14581 tgcaaggctg ccggaccgac gacatcatca cgcgaagagc cttatcagtg ggaagtcatg
    14641 cgcgactaca agattcgcgg cgtcatcggc aagggtggga tgggtgagaa gacgctggcg
    14701 gcctgcaaag agtacggcgc ggtctacctg cacgccatcg gcggcgccgc ccaggtctgc
    14761 gctgcctcga tcaaacgtgt cgacggcgtc gactggatgg acctcggatc acccgaagcg
    14821 atctggcatc tgtgggtcga agacttcatg gccatcgtca cgatggacgc acatggccag
    14881 tcgctgcaca agaatgtctt cgaggactcg aaggcgcggc tcgaagcgat gaaggctcgg
    14941 tgatcgggca cgccgaaccg cgagccgaac cgaggcgaag gcctatactc ggctcttcca
    15001 atgaaacacg ccgcgatcga gccgtccacg cgggtgtggg cagcgcctga ttttcaatcg
    15061 atcgccttcg gacgcgaggg cgagatcctc gccgcaaagg tcaggctcgg catcatggcc
    15121 ctcatcggcc tgattccgct gcagaccgta cttttccggg ccgccaacgc agaagcgaag
    15181 atcggcctcg gtgtaacaat cctcgtcctc gtcatgggca ccgtcgtgct cagggtagcc
    15241 cagcgaccga aaccacctgc atggctcggg ctcttcacct gtcttctcga cgtcagcacg
    15301 gtcagcatgg tcaacgccgg cttcctgctg tccggcaatc ccctcgcggt taccaacagc
    15361 cgcgtcgtct tctgctgcta tttcgtggca ctggcgctgg tttgtctacg gcaggattgg
    15421 cggtggtgcc tgatcgccgc gaccgtggca atgatggagt atggctgcat cgtcgcctgg
    15481 gccgcagcgc gttatgacct ttacggcgcc gcgtttgcga cgagcggtta cggccggttc
    15541 aactgggaca atcaaatcgc ccggttgatg ctgttggctg cggccggcgt gatcggcgcc
    15601 gtgaccgtag tgcagagccg cggctactgg gaagcagtga tcaagtatct cgatgccttc
    15661 ccgctcggcg tcatcatcgc cggggctgat cacaggtcgc ggtacgccaa ccgcgccgcg
    15721 caggagcttc tgaaccgcga cataccgccc ggcgtgcagt tgtcggagct caacgggcag
    15781 ttgttcctct ccggcaccga cgatccatat ccgccgcgac aaaccacgat cgcgctggcg
    15841 ttggcgggag aatccaccga atgtgatgac atcgagatcc aacggcccgg ctcgcgcatc
    15901 gcggtcgagg cctggggaac tccggttctc gattccaaag gccgcgtctc caatgcaatc
    15961 gcggtgttcc acgatgagac gcggcgccgg cgcgccgagg aggagatgcg gcgcctcggg
    16021 gagatgcttc gcgaagccaa cgccgagctc gcgcttcgcg gcgtcattct cgagtccgtg
    16081 gccgtcgccg ccgaacggct gttccagtcc gcctcgtggg aagaagtggc gaccgaagta
    16141 atcggcggca ttggaagtgc ggtgggagcc agccgagcat cgatcttcca ggtctccgac
    16201 gatggcgagc tcgctgcgcg gttgcgattc gggtgggtgg cgccgggagt cgttccgatc
    16261 ggcgaagcga tgcgccagaa agtccccttc cgcgtctcgg gtttcggccg ctggctcgat
    16321 agcttcagcc gcggcgagac cgtgcacgga ccggtgcggg actttcccga gagtgagcaa
    16381 accgtgctga cgccgcaggg agtacgctcc cttgccgtgg tgccggtgca tttgggcgag
    16441 cgtctctggg gattcctcgg cttcgacgac tgcgtgtctg atcgtcagtg gtcgcaaggc
    16501 gagatccttg gtctgcgcgt tgcggccgat atgctcgccg aggcgattgc tgcgaaggag
    16561 cgggcagcgg cgatcgaggc aagcgaggaa cgctaccgca cccttttcga gcgcagcctc
    16621 ggtctgcttt gcacgcactc gatggacggc acgatcctca ccgtcaacca tgccgcagcc
    16681 tcgaggttgg ggctaacgat agaccagatg atcggccgca atctcgagca ctttctggcg
    16741 ccgagcgccc tcgcgaagtt ccccgagtac ctggcagcga tcgcagcgac gggccaggcg
    16801 agcggcaacc tcgtcctttc cgataccaac ggaaccgagc gggtgtggga gtaccgcaac
    16861 tacgtcgtcc gcgagcccaa ccaggaggct tacgtgctcg gacacgcgct cgacgtcacc
    16921 gaccgcacga cgctggagcg agagttgcgt gaccgggctc tgaaggaccc gctgaccgga
    16981 atggccaacc gcacactttt cgaggaccgc ctcacgcgcg cgttcgagcg cgcgaaacgg
    17041 agggccatcg cggaagagga aacgcagccg tgggcgctcg cgtatctcga cctcgacggc
    17101 ttcaaagaaa ccaacgatcg attcggccat cctgcgggcg acgctctgct gtgcgaagta
    17161 gccacacggc ttcgccacgg cctgcgagct ctcgacacgg tggcgcgact cggcggcgac
    17221 gagttcgcgc tcatcctccc cgacatccgc tcgcaggaaa atgcgaagca tgtgatcgac
    17281 aagctcatgg agactctgcg ctatccgttc ctctacagcg gatacacgct cgtggtgagc
    17341 gtgagtgtgg gagtcagcat gcatcccctc gatggagaat cgaccgaagc gctgaccgcg
    17401 cgcgctgaca aggccatgta cgccgccaag tcggccggcg gcagcggcta ccgcttctat
    17461 agtgacctct gaccatgcgc accgaacgcg acaccctcgg cgaagtccag gtccccgatg
    17521 acgcgcttta cggcgcgcag acgcagcgcg ccgtcgagaa ctatccgatc agcggactgc
    17581 gcgcccatcc ggttttcatc cgctcgttca tccttctgaa gagagcggcc gcgctggcga
    17641 accgggagct cggcacgctc gacgccgctc tctctgatgc catcgcgcgc gcctgcgacc
    17701 gccttctcgg cgacgacgaa ggcaatcgga aacacttccc tgtcgacgtc ttccaggccg
    17761 gcgcaggcac gtcgttcaac atgaattgca acgaggtgat tgcgaacctc gccaacgtct
    17821 cgctcagcgg acacctcggc gagtacaagc cgatccatcc gaacgaccac gtgaacatgg
    17881 cgcagtcgac gaacgatgtc ttcccgacgg cgatccgaat cgccacgctt ctcctgctgc
    17941 gcgagctgac aacccaactc gatgcgctcg ccgccgcatt cgacgatcgc ggtaacgcgt
    18001 tcgcggacgt gatcaagtcg ggccgcacgc atttgcagga cgccgtcccg ctcaccctcg
    18061 gccaggagtt ccgcgcgtac gcatcggcca cgcggcgcgc cacgcgattg ctcacggcca
    18121 gcgccgccga actgcgcatc ctcggcatcg gcggcaccgc cgtcggcacc ggaatgaacg
    18181 cgccccgcgg ctaccggcaa gtcgtcgtcc gcaacctgaa cgcgttgacg aatctcgatc
    18241 tcgtgccgac cgacgatcta cgcgaggcga tgcaatcgca gcttcccgtc tccgccgtgt
    18301 ccggggctct gcgcaacctc gcgctggagc taacgcggat cacgaacgac cttcgccttc
    18361 tcgcttccgg cccgcagacc ggcctcgccg agatcacgct gccgtccgtt caacccggtt
    18421 cctcgatcat gcccggcaaa gtaaatccgt cgatgctcga gtgcatgaac caggttctct
    18481 tccacattat cggctcggcg accacgatcg actatgcggt gcaggccggt cagctcgagc
    18541 tgaacgtgat gatgccgctg atggcgttcg agatcctctt ctcgatcgag atcctgaaga
    18601 actatctgcc cgtcttcatc gagaagtgca tccgcgacat cgaggcggac cgcgcgcagt
    18661 gcgaggcgta ctacatcagc tcgccgtcgc tggccacggc
//
LOCUS       DATLVW010000599         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_194738, whole genome shotgun sequence.
ACCESSION   DATLVW010000599 DATLVW010000000
VERSION     DATLVW010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194738"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1755
                     /locus_tag="VNN25_17750"
     CDS             169..1755
                     /locus_tag="VNN25_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXH93431.1"
                     /translation="MSSFTDAASRVERACDFFDRRAAAQASAELAAEIRSGKRVFEAD
                     LAMKSLKRLRKNRYFEAALDVAEAVLQTGQTDNLVRTIYAQALIDSGHLIAALSFLSE
                     VFPSVTRGSVGENEVGGLIGRTYKQLFVESAGEAALREDRLRRAFDTYYDAYVDAPER
                     YWHGINAVALLARAEKEQVTLRRVAPFAEKIFRQTAALAESSNSDPWPMATAAEAALA
                     LGDFEESRKRYGLFASFDRTIVGAFEIYSALRQLIEVWGLTDDQEPGATLLPLLRAAY
                     LDRSGGDVAFEGDGFRRNVERVSSLLEKTFSETMFRTLKWYRSGLERCNPVCRINDPQ
                     GAGIGTGFVVRGGDFNDGLGDDKFVLTNAHVLSNSYPNALAEGDAVATFEALGETGEY
                     TLEHIVWSDSQLDATLARASKPLPTCGTYKIANKLPLNDQKQRVYVIGHPNGAGLSLS
                     LNDNVLLDYDDRVVHYRAPTEGGSSGSPVFNADWKLIALHHMGDFNARRLNGLAGTYA
                     ANEGIRIDRIIETTKKAKMI"
     gene            1776..>2411
                     /locus_tag="VNN25_17755"
     CDS             1776..>2411
                     /locus_tag="VNN25_17755"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HXH93432.1"
                     /translation="MLPQIVERLKLFVASPGDVQRERDHVVAVVDELNRGIAPQAGYT
                     LEAVRWQTHARPDMGRAQQVIFNQIGDADLFVGIMWQRFGTPSGVADSGTEEEFEYAL
                     GHWERERRPRCLFYFGAAPIVPPQSVDAAKQLLKVAEFRERIGRIALAFRYDGDAHFK
                     DMLREHLQQVLMQEFPRSRPPLDPNLEALLELEKQRCRQADVEFKTPNLLLA"
BASE COUNT          457 a          772 c          761 g          421 t
ORIGIN      
        1 tcccgtcgtc ggcttcgacc cgcagttcgc gaacgatttc gagcgcggcg gccagcaggc
       61 tgtgctggat gtcaatgaac aaaactccgg cgcctggcga cacaacatca gccattacct
      121 caggggcgag aagctgcgtg cccgccttgg cgaattgctg gagctgcaat gagctcattt
      181 accgatgcgg cgagccgtgt cgagcgtgcg tgcgatttct tcgaccgccg cgccgccgcc
      241 caagcgtccg ccgagctcgc cgcggagatc cgctccggca aacgcgtctt cgaggcagac
      301 ctggccatga agtcgctcaa gcgccttcgg aagaatcgct atttcgaagc cgcactcgac
      361 gtggcggagg cggtactcca gaccgggcag acggacaatc tggtgcgaac catttacgcc
      421 caggcgctca tcgacagcgg gcatctcatc gccgcgctct cttttctttc cgaggtcttc
      481 ccctcggtga cgcgcggctc cgtaggcgag aacgaagtgg gcggacttat cggccgcacg
      541 tacaagcagt tgttcgtcga gtcagccggc gaggctgctc tccgcgaaga ccgcctgcgc
      601 cgcgccttcg acacgtatta cgacgcttat gtcgatgcgc cggagcgata ctggcacggc
      661 atcaacgcgg tggcgttgct cgcccgcgcc gaaaaagaac aggtcactct tcgccgggtc
      721 gcgccgttcg ccgagaagat atttcggcag acggcagcgc tggcggagtc ctcaaacagt
      781 gacccatggc cgatggccac cgccgccgag gccgcgctcg cgctcggaga tttcgaggag
      841 tcgcgaaaac gctacgggtt gttcgccagc ttcgaccgga ccatcgtcgg cgcgttcgag
      901 atttacagcg cgctgcggca actgatcgag gtgtggggcc tcaccgacga tcaggagccc
      961 ggagcgacgc ttcttccgct gctgcgcgca gcgtacttgg accgatccgg cggcgacgtg
     1021 gcgttcgaag gagacggctt ccggcgcaat gtcgagcggg tctcttcgct gctggagaag
     1081 accttcagcg agacgatgtt caggacgctg aagtggtatc gctccggctt ggagcggtgc
     1141 aatcccgttt gccggatcaa cgatccacag ggcgccggaa tcggaacggg gttcgtggtg
     1201 cgcggcggcg acttcaacga cggactcggc gacgacaagt tcgtgctcac gaacgcgcac
     1261 gtcctcagca attcgtatcc gaatgcgctc gccgaggggg acgcagtggc gacgttcgag
     1321 gccctcgggg aaacgggcga atacacgctc gagcacatcg tctggtccga cagtcagctc
     1381 gacgcaacgc tcgcccgcgc ctcgaagccg ctccctacct gcggaaccta caagatcgcg
     1441 aacaaactgc cgctcaacga tcaaaagcag cgcgtctatg tgatcggcca tccgaatggc
     1501 gctggcctgt cgctttcgct gaacgacaac gtgctcctcg attacgacga cagagtcgtc
     1561 cactatcgcg ccccgaccga aggcggaagc tcgggcagcc cggttttcaa tgccgactgg
     1621 aagctgatcg ccctgcacca catgggcgat ttcaatgccc gccgactcaa tggtctggcc
     1681 ggcacctacg ccgcaaacga agggattcga atcgaccgga tcatcgagac cacgaagaag
     1741 gccaagatga tctgacctcg tcgataatgg cgtccatgct gccgcaaatc gtcgaacgac
     1801 tcaaactctt tgtcgcctcg cccggcgacg tgcagcgcga gcgcgaccat gtcgtcgccg
     1861 tggtcgatga gctcaatcgc ggcattgccc cacaggcggg ctacacgctg gaagcggtgc
     1921 gctggcagac gcacgcccgc cccgacatgg gccgcgcgca gcaggtgatc ttcaatcaga
     1981 tcggcgatgc cgatctcttc gttggcatca tgtggcagcg cttcggaaca ccctccggcg
     2041 tggccgactc cggcacggag gaggagttcg aatacgcgct cggtcattgg gagcgcgaac
     2101 gccggccgcg atgcctcttc tacttcggcg cggcaccgat tgtcccgcca cagagcgtgg
     2161 atgcggcgaa gcagctgctc aaggtggccg agtttcgcga gcggatcgga cggattgccc
     2221 tggccttccg gtacgacggc gatgctcatt tcaaagatat gttgcgggag catctgcagc
     2281 aggtcctgat gcaggagttc ccgagatcgc gaccgccgct cgaccccaat ctggaggcgc
     2341 tgctcgagct ggagaagcag cgctgccgcc aggccgatgt ggaattcaag acgccaaacc
     2401 tgctgctggc g
//
LOCUS       DATLVW010000600         8094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_360127, whole genome shotgun sequence.
ACCESSION   DATLVW010000600 DATLVW010000000
VERSION     DATLVW010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360127"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VNN25_17760"
     CDS             <1..258
                     /locus_tag="VNN25_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93433.1"
                     /translation="VRHNGSVAANIDPNSPVPPVGKTLPAFTVQGTGLGVRVFQTAGV
                     SNEVTFWVENLTNQLYAEFSNATFFRPEPGRTMKVSYRVKF"
     gene            492..1211
                     /locus_tag="VNN25_17765"
     CDS             492..1211
                     /locus_tag="VNN25_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH93434.1"
                     /translation="MPTPYNLDVHNDCSECDVRAGRTFCNLAPETLAELDTIKFTGIY
                     PKGSLLFVEGEQPRGVFLLCAGRVKLTTSSSEGKTLIVKIAEVGEVLGASATILGKAY
                     EVSAETIEPSQLNFIKREDFVRFLSSHVDVCMHTAQQLSEKYQSAQREIRSLGLSQTT
                     SEKLARLLLDWCTKNGEQTTRGIRLSVLLTHEEVAQMIGTTRETVTRIFSDFKKRQII
                     EVKGSNIFVLAKPKLEAMIGS"
     gene            1359..2153
                     /gene="ubiE"
                     /locus_tag="VNN25_17770"
     CDS             1359..2153
                     /gene="ubiE"
                     /locus_tag="VNN25_17770"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXH93435.1"
                     /translation="MADDRPTPADNARQRNQPTTDNALSPRASAAATQYTGPDPTRIK
                     TMFGSIAGRYDRANTVLSGPVHHLWRRTAVRWSGVKRGDAVLDCATGTGDLAIAFKKA
                     VGPDGRVVGTDFTPEMIELARGKAPGITFEVADVTALSFDDDSFDIASISFGIRNVGD
                     PAKGIAEMARVVRPGGRVIVLEFGQPSNRLASALYNFYRRHFLPRLGGAVTGERSAFE
                     YLESSAGKFPCGDEFVAMMRNAARFTSIDVKPLTFGIAYLYRGVVS"
     gene            complement(2163..3581)
                     /locus_tag="VNN25_17775"
     CDS             complement(2163..3581)
                     /locus_tag="VNN25_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA Peptidase M64"
                     /protein_id="HXH93436.1"
                     /translation="MRKLLFVAALFLAASSAFAARFEEMFLDKTMRVNYFHTGNHGEE
                     VIALDSVVSDGPWPGSRSRLIDTMNLGNYYFEVIDRETNQPVYSRGFASVYGEWQTTD
                     EAKERAGTFAESVRFPWPKKPVQLVVKKRDDKGAFQQIFSTLIDPSSRFVNPSDRKPA
                     GHVWNVIENGPASEKVDLLVIGEGYTESELPKFHKDVERMIGKLFATEPFKSRRKDFN
                     VRALDLAEPASGVHRPRTNDNRRTMTGVQYNIFDSERYVLTLDDHRMRDVASSAPYDF
                     IEILVNEKQYGGGGIFNDQATACVDSAFAEYVFVHEFGHHFAALADEYYTSPVSYTSA
                     GGSEHIEPWEPNATALHDPANVKWKELIDTSTPLPTPWDKETFEKHSREIQKERARLR
                     ATNAPESEMDKLFTAERTWETQFLGSQKFAGKVGAFEGAAYEAQGLYRPELDCIMFTR
                     DEVGFCRVCRKAIERIIDMYSK"
     gene            3674..4705
                     /locus_tag="VNN25_17780"
     CDS             3674..4705
                     /locus_tag="VNN25_17780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH93437.1"
                     /translation="MRALLIALVLFATSLHAQMQEQITVERILVDARVTDDHGNPITG
                     LKAGDFLVRIDGKPARVESAEWIPETAAARELASIDAAIADVNTTLDQPAPHGRLLIF
                     FYQTDFERNESRVAGQMQTVVNADDWLDWLDPEDRVAVFSFDSHLKFRLDFTNNKDHI
                     KDAMKQALYTDDPPPPRMVPMPALAKRLDPIEMRKAAAPEAALIILANALRPIPGPKS
                     IILFGWGLGRLTPSGVMMESKYIPARQALESARASVFSIDFTQADFHSLEAGLGKVAD
                     DTGGFYAKTFHFPKIAVERLQKTLAGHYELEVRKPDTKVRGTHSIEVDVPSQRRASIL
                     ARSTYVDHD"
     gene            4760..4957
                     /locus_tag="VNN25_17785"
     CDS             4760..4957
                     /locus_tag="VNN25_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXH93438.1"
                     /translation="MATKYGKKASDKVEKAMHERKKGTLKSGSSGKKVTSKKQAIAIG
                     LSEARRAGGKVPSKKSSSKKK"
     gene            5056..5718
                     /locus_tag="VNN25_17790"
     CDS             5056..5718
                     /locus_tag="VNN25_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HXH93439.1"
                     /translation="MTNRILLTIFAAFFVWSLIHPHDYFTWFLEVFPAIIGIALVIAV
                     RKSFPLTPLLLTLLTIHAIILMIGGHYTYAEVPLGFWIRDALHMARNDYDRIGHFAQG
                     FVPAIAAREILIRRNVVRSRGWRYYIIVSICLAISAAYELLEWRVSVATGSAGDSFLG
                     TQGDVWDTQEDMATALVGALMAPLVLGWLHYRQLAALQPARPLVPEPASPAPDRERLE
                     NR"
     gene            complement(6052..7089)
                     /locus_tag="VNN25_17795"
     CDS             complement(6052..7089)
                     /locus_tag="VNN25_17795"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH93440.1"
                     /translation="MRKTTKAAIAIGSAFGVAAIERIFFAAPRWRGAITDHFDGERFR
                     NREDGWQSEGSFLKWMANRERGAWPEHPELAYGPQPPERVGDGRLRITFINHSTTLIQ
                     IDDVNILTDPVWSERVSPVSFAGPKRHRAPGIRFDDLPPIDLVLVSHNHYDHMDVATL
                     RALQKHHRPAIVTPLGNAALLRRHGVHDAFELDWWETAHHADGIKTTVVPARHFSARA
                     MSDRNRNLWSGFVISAPSGHIYFAGDTGWGNHFAEIGQRFSPIRAALLPIGSYMPRWF
                     MQPAHIDPAQAVDAHFALGAQTSVAVHFGTFALGDDGEYDPIHDLNEALAANGNPPFL
                     VLGQGEGRDLP"
     gene            7466..8059
                     /locus_tag="VNN25_17800"
     CDS             7466..8059
                     /locus_tag="VNN25_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93441.1"
                     /translation="MQKKRAVLATAIALVALLALGSTPTGMAPLPGQYLRAGTFHSPD
                     GWFQIHVPTNWEWFEMRKFDGEADPRCPDAINDSVGWLARDPKTFDDLVVMESYRPGG
                     DIITESYATSFESNTRKAVAPETMSDYSSELLTISGERSLHYRYKLVRKNHPPTYRFG
                     YITGMEHKLTLSTSDDKPQEPKRLWQIVVSISWLKEP"
BASE COUNT         1611 a         2601 c         2428 g         1454 t
ORIGIN      
        1 gtccgccaca atggttccgt ggccgcgaat atcgatccca actcgccggt acctcccgtc
       61 ggaaagacgc ttcccgcgtt caccgtccag ggcacaggcc tcggcgtgcg cgtcttccaa
      121 acggccggtg tctcaaacga agtcacgttc tgggtcgaga acctcacgaa ccagctctac
      181 gccgaattct ccaacgccac gttcttccgt ccggagccgg gaaggacgat gaaggtcagc
      241 tatcgcgtca aattctgaag ggcagggaag gcagagatca gagacgaaga gatcagagac
      301 gaagagatca gagacaagtg gtacccctga tccccaagtc tctgatctct gatatctctg
      361 atctctgctt cctgatctct gcttcctgct ctctgactca atcatgtgac attcatcact
      421 ccgaacggtg agattcagcg ccgcccttag cgacaagcga ctctatattc ggtgacgggg
      481 gttttctaac catgccgaca ccgtacaacc tcgacgttca caatgactgc tccgaatgcg
      541 atgtgcgcgc ggggcggacg ttctgcaatc tggcgcccga aacgcttgcg gagctcgata
      601 ccatcaaatt cacggggatc tatccgaaag gttcgctgct gtttgtcgaa ggcgagcagc
      661 cacgaggagt tttcctgctc tgcgccggcc gcgtgaagct cacgacgtca tcgtccgaag
      721 gaaagacgct gatcgtcaag atcgccgagg tgggggaggt tctcggagca agcgccacga
      781 tcctcggcaa agcctacgaa gtctccgccg agacgatcga gccgtcgcaa ttgaacttca
      841 tcaagcgcga agatttcgtc cgcttcctca gctcgcacgt cgacgtctgc atgcacaccg
      901 cgcagcagct cagcgaaaag tatcagtccg cccagcgcga gatccgctcc ctcggcctct
      961 cgcaaacgac gtcggaaaag ctggccagac tgctgctcga ctggtgcacg aagaacggcg
     1021 aacagacgac aagaggaatc cgcctcagcg tcctcctgac ccacgaagaa gtcgcccaga
     1081 tgatcggcac gaccagagag accgtcaccc gcatcttcag cgatttcaag aaacgccaga
     1141 tcatcgaagt caaaggctcg aacattttcg tcctggccaa accaaagctg gaagcaatga
     1201 tcggctcgta actttcactg atcccggacc aaatgcgcgc ggaacgccgc cgtctcggcg
     1261 gctggcccgg cggcgtcctc gccgccgacc gcgttgtctt gcgtaaacag ggcacgcagt
     1321 cgctcagcag atggtaacgt tcgccgccca agaagctcat ggccgacgac cgccccacac
     1381 ccgctgacaa cgcgcggcag cgcaaccaac cgacaaccga caacgcgttg tcgccgcgcg
     1441 ccagcgcggc ggcaacgcaa tacacaggtc ctgatcccac acgcatcaaa accatgttcg
     1501 gctccatcgc cggccgctac gaccgcgcga acaccgtcct ctccggccct gtccatcacc
     1561 tgtggcgccg caccgcggtg cgctggtcgg gcgtgaaacg cggtgacgct gtcctcgact
     1621 gcgcgaccgg aaccggcgat ctggcaatcg ctttcaaaaa ggcggtaggt ccagacggcc
     1681 gcgtcgtcgg aaccgacttc actcccgaga tgatcgagct cgcccgcggc aaggcgccag
     1741 gcatcacgtt cgaagtcgcc gacgtaaccg cgctgtcgtt cgatgacgac tcattcgata
     1801 tcgccagcat ctccttcggc atccgcaacg tcggcgatcc cgcgaaaggc atcgctgaga
     1861 tggcgcgcgt ggtccgtccg ggcggacgcg tcatcgtgct cgagttcggc cagccatcga
     1921 accggctcgc ctccgcactc tacaatttct accgccgcca cttcctcccc cgcctcggcg
     1981 gcgccgtcac cggcgagcgc agcgccttcg agtacctcga atcgtcagcc gggaagtttc
     2041 cctgcggcga cgagttcgtc gcaatgatgc gcaacgccgc gcgattcaca tcgatcgacg
     2101 taaagccgct gaccttcggc atcgcgtatt tgtatcgcgg cgtggtttcg taaacgtttt
     2161 cgctacttcg aatacatatc aatgatccgc tcgatcgcct ttcggcacac gcggcagaag
     2221 cccacctcgt ccctcgtgaa catgatgcag tccaattccg gacgatagag cccctgcgcc
     2281 tcgtacgccg ccccttcgaa tgcgccgact tttccggcga acttctggct gccgaggaat
     2341 tgggtttccc aggtgcgctc ggcggtgaag agcttgtcca tctctgactc cggcgcattc
     2401 gtcgcgcgga ggcgggctcg ctccttctga atctcgcggc tgtgcttctc gaacgtctcc
     2461 ttgtcccacg gcgtcggcag gggagtggaa gtgtcgatca gctccttcca cttcacgttg
     2521 gcgggatcgt ggagcgcggt cgcattcggc tcccacggct cgatgtgttc gctgccgccg
     2581 gcgctcgtgt acgagaccgg cgaggtgtag tactcatccg cgagcgccgc gaagtgatgt
     2641 ccgaactcgt gcacgaacac gtactcggca aacgcgctgt cgacgcatgc cgtggcctga
     2701 tcgttgaaga ttccgccgcc gccgtactgc ttctcgttga cgaggatctc gatgaagtcg
     2761 tacggcgccg acgacgccac atcgcgcatg cggtgatcgt cgagcgtcag cacgtagcgc
     2821 tcggaatcga agatgttgta ctgcacgccg gtcatcgtgc ggcggttgtc gttcgtgcgc
     2881 gggcggtgaa cgccgctggc cggctcggcc agatcgagcg cgcggacgtt gaagtctttg
     2941 cgccggctct tgaagggctc cgtggcgaag agctttccga tcatgcgctc gacgtccttg
     3001 tgaaacttcg ggagctccga ttcggtgtat ccctcgccga tgacgaggag atcgaccttc
     3061 tccgatgccg gtccgttctc gatcacgttc cagacatgac cggcgggttt gcggtcggac
     3121 ggattcacga agcgcgaaga cggatcgatc agcgtcgaaa agatctgctg gaacgcgcct
     3181 ttgtcgtcgc gcttcttgac gacgagctgc accggcttct tcggccacgg aaaacgcacc
     3241 gactcggcga acgtgccggc gcgctccttc gcctcatcgg ttgtctgcca ttcgccgtag
     3301 accgaggcga agccacgcga gtagacgggc tggttcgtct cgcggtcgat cacttcgaag
     3361 tagtagttgc cgaggttcat ggtgtcgatg aggcggctgc ggctgcctgg ccatggaccg
     3421 tccgagacca ccgaatcgag cgcgatgact tcctcgccat ggttccccgt gtggaagtag
     3481 ttcacgcgca tcgtcttgtc gagaaacatc tcctcgaagc gggcggcgaa cgcggaggaa
     3541 gcggcgagaa acaacgcggc gacgaaaaga agtttgcgca tgtggagatt gtagttgtga
     3601 acgatctgcg tcgaactgcg gaagcgtttt atccgcagat gtcgcagatg gggcgcagat
     3661 gtagactgcc gggatgcgcg ctcttctcat tgcgctcgtg ctgtttgcta cctcgctgca
     3721 cgcccagatg caggagcaga tcaccgtcga gcgcatcctc gtcgatgcgc gcgtcacgga
     3781 cgatcacggc aatccgatca ccggactgaa ggccggcgac tttctggtgc gcatcgacgg
     3841 caagccggcg cgcgtggaat ccgcggagtg gattccggag acggcggcgg cgcgcgagct
     3901 ggccagcatc gatgcggcaa tcgccgacgt gaacacgacg ctcgatcaac ccgcgccgca
     3961 cggacgcctc ctcatctttt tctaccagac cgatttcgag cgcaacgagt cacgggtggc
     4021 cggacagatg cagacggtcg tcaacgccga cgactggctc gactggctcg atcccgaaga
     4081 ccgcgtggcc gtgttctcgt tcgactcgca tctcaagttc cgtctcgatt tcacgaacaa
     4141 caaggaccac atcaaggatg cgatgaagca ggcgttgtac acggacgatc cgccgccgcc
     4201 gcggatggtg ccgatgcccg cgctggcgaa gcgcctcgac ccgatcgaga tgcgcaaggc
     4261 tgccgcgccg gaagcagcat tgatcatcct ggccaacgcg ttgcggccga taccgggtcc
     4321 gaagtcgatc atcctcttcg gctggggact cggccggctc acgccgagcg gcgtgatgat
     4381 ggaatcgaag tacatccccg cgcggcaggc gctcgagtcc gcgcgtgcca gcgtcttctc
     4441 gatcgacttc acgcaggccg atttccactc gctcgaagcg gggctgggga aagtcgcgga
     4501 cgataccggc ggcttctacg cgaagacatt tcatttcccg aagatcgcgg tggaacgcct
     4561 gcagaaaacg ctggccggac actacgagct cgaagtgcgc aagcccgata cgaaagtccg
     4621 cggcacgcac tcgatcgaag tcgacgttcc atcgcaacgg cgcgcgtcga ttctggcgcg
     4681 cagcacgtac gtcgaccacg attgatcggg caaacgactt gcactccttc ctgtgcagaa
     4741 tcgaaaggag attttgcgca tggcaaccaa gtacgggaag aaggcgtcgg acaaagtcga
     4801 gaaggcgatg cacgagcgga agaagggaac gctgaaaagc ggatcgtccg gcaagaaggt
     4861 gacgagtaag aagcaggcca tcgccatcgg gttgtccgag gcaagacgag caggcggcaa
     4921 ggtgccgtcg aagaagtcat cctcgaagaa aaagtagaaa ggcttttcac gcggagacgc
     4981 ggagaacacg gagaaacccc tccgcgtcct ccgcgtctcc gcgtgaaacg gtttgaaagc
     5041 ccgtacactg agcgagtgac gaaccgcatc ctcctcacca tcttcgccgc gttcttcgtc
     5101 tggtcgctca tccatccgca cgattacttc acctggttcc tggaagtctt tccggccatc
     5161 atcggcatcg cgctcgtcat cgcggtgcga aagagttttc cgctcacgcc gctcctgctc
     5221 acgctgctga cgattcacgc catcatcctc atgatcggcg ggcactacac gtacgccgag
     5281 gtgccgctgg gattctggat acgcgacgcg ttgcacatgg cgcgcaacga ctacgaccgc
     5341 atcggccact tcgcgcaggg cttcgtgccc gccatcgccg cgcgcgagat cctcatccga
     5401 cgcaatgtcg tgcgcagccg cgggtggcgc tactacatca tcgtgtcgat ctgcctcgcc
     5461 atcagcgccg cgtacgagtt gctggagtgg cgtgtcagcg tcgctaccgg cagcgccggc
     5521 gactcattcc tcggcacaca gggcgacgtc tgggacacgc aggaagacat ggccaccgcg
     5581 ctcgttggcg cgctcatggc gcctttggtt ttgggctggc tgcactatcg gcaactggcg
     5641 gcgctgcaac cggcgcgtcc actggtgccg gaaccggcgt cgccggcacc cgaccgcgaa
     5701 cgcctcgaaa atcggtgagc gcccagagca ccagcatcag caatcccatc atccagtaga
     5761 aacgtggcga ggtgacgccg ctggtgaaac tgccgaacca cggagccgca atcacacagg
     5821 cgatcagcag caactccacg aatccgtgaa gagcgagtcc aatgactttc acgacgccga
     5881 gcgggtagcg cgtcagcagc gccatcgcga acagcaggcc gccgaagatg tacgcccacg
     5941 tcgccttcac ccctgcgaag ccggccaccg acggcccgat gatcagcatg atggccaggg
     6001 cgtagtcgag gatgccgtgc gataccgctc caatgctgcg atctctgcgt ctcatggcaa
     6061 gtcgcgtcct tctccctggc cgagtacgag gaacggcgga ttgccgttcg cggccagcgc
     6121 ctcattcaag tcgtgaatcg gatcgtactc gccatcgtcg ccgagtgcga atgtcccgaa
     6181 gtgaacggcc acgctcgtct gcgcgccgag ggcgaagtga gcgtcgaccg cctgggcggg
     6241 atcgatgtgc gccggctgca tgaaccagcg cggcatgtac gagccgatcg gcagcaaagc
     6301 ggcgcggatg ggggagaagc gctggccgat ctccgcgaag tgattgcccc atccggtgtc
     6361 gccggcgaag taaatgtgcc ccgagggagc gctgatcacg aagccgctcc agagattgcg
     6421 attgcggtcg ctcatcgcgc gcgctgagaa gtgccgcgcc ggaacgacgg tggtcttgat
     6481 cccatccgca tgatgcgcgg tctcccacca gtcgagctcg aacgcgtcat gaacaccgtg
     6541 ccgccgcagg agtgccgcgt tgccgagcgg cgtgacgatg gccggccggt gatgtttctg
     6601 cagcgcgcgc aacgtggcca cgtccatgtg gtcgtagtgg ttgtggctga cgagcacgag
     6661 atcgatcggc ggcagatcat cgaagcggat ccccggcgca cgatgccgct tcggccccgc
     6721 gaacgaaaca ggcgagacgc gctccgacca gaccgggtcg gtgaggatgt tgacgtcatc
     6781 gatctggatg agcgtcgtcg agtgattgat gaaggtgatg cgcagccgcc catcgccaac
     6841 ccgctccggc ggctgaggtc catacgccag ctccggatgc tccggccacg ccccccgctc
     6901 ccgatttgcc atccacttca gaaacgaccc ctcggactgc cagccatctt cgcgattgcg
     6961 aaaccgctcg ccgtcgaaat gatcggtgat agcaccgcgc catcggggag cagcgaagaa
     7021 gattctctcg atcgctgcca ccccgaacgc agacccaatc gcgatcgccg ccttcgtagt
     7081 tttccgcatc gccgtgtatt cgaacacaag tgcagaaagc acaccggaag gaacactcaa
     7141 cattcgtgtc atcctgagac agccggagcc gccctctgtg ctcgatcaag tttgcgcagc
     7201 gtgatgtggc ggcgtaggac ggcgaaggat ctcaagttgc cgggaacatg gcatgcgcgt
     7261 cgtaattaga gatccttcgc cgtcctacgc cgccgtgcac attctccgcg aacggatcga
     7321 ttcgcgccgg cggctccggc agtctcagga tgacacgatg gatgacacga ttgatgagac
     7381 gtggatggat gacgaacgtc gcgaaaactc gcctctcgtg actaccatca ctcgctcgat
     7441 gcgacgccgg ccgaatactc gcgcaatgca aaagaaacgc gccgtcctcg cgaccgcgat
     7501 cgccctcgtg gccttgctcg cgctcggttc cacgcccacc ggcatggcgc cgctgccggg
     7561 gcaatatctt cgcgccggca ccttccattc gccggacggc tggttccaga tccacgtgcc
     7621 tacgaactgg gagtggttcg agatgcgcaa attcgacggc gaggccgatc ctcgctgccc
     7681 cgatgcaatc aacgactccg tcggatggct cgctcgcgat cccaaaacgt tcgacgatct
     7741 cgtcgtgatg gagtcgtaca ggcccggcgg ggacatcatc acggagagct atgcgacgag
     7801 cttcgagagc aacacacgca aggccgtcgc gccggaaacg atgtccgatt actcatcgga
     7861 actgctcacc atctccggcg agcggtccct tcactatcgc tacaagctcg tgagaaagaa
     7921 tcacccgccg acctatcgct tcggctacat caccggcatg gagcacaagc tcacgctatc
     7981 aacctcggac gacaagcccc aggagccgaa gcgtctctgg cagatcgtgg tctccatcag
     8041 ctggttgaag gagccgtaag accggtcacg cgaaggcgcg aagacgcgaa gggg
//
LOCUS       DATLVW010000601         5233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_70200, whole genome shotgun sequence.
ACCESSION   DATLVW010000601 DATLVW010000000
VERSION     DATLVW010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70200"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VNN25_17805"
     CDS             <1..440
                     /locus_tag="VNN25_17805"
                     /inference="COORDINATES: protein motif:HMM:NF027969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HXH93442.1"
                     /translation="VEFAPIPRFAPEHFAVLRAKQVTRQKQFQKGLAQLEEEGVMQVL
                     SAHEGLRSEPILAVVGELQFDVMQSRLESEYNVETTVVALPYKLARWVDRDVHDVATK
                     MNLPSRSKIATDSAGRAVVLFSSQWELDYAEKENPGVTFLSAA"
     gene            complement(564..1850)
                     /locus_tag="VNN25_17810"
     CDS             complement(564..1850)
                     /locus_tag="VNN25_17810"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HXH93443.1"
                     /translation="MQITSSQALEAAASAWYGSNIRSARGERLRVPADALQLVSEAAT
                     QVVEANSAQSSRDGRAVDVLAPSVGHAACFMVAANLLGRMIPGSYSDLDLIDSTFGDR
                     PVYRGQARPWHLRPTGWRRTFPTDRAIAAMRGHIGKFMTAEDAIELDLIGRPENDSEF
                     AGLAQHYGVPTSLVDFTFDPLIALLFACAADHVAFDEQSVPPDHAVIYTTTLAKLVTV
                     RNVIMHFPPLQARRLYRQHGLFVDFGALPAGGAVDFEVAANWTAVEQSCVRIFVPREY
                     PIAIEMSALRIDDVLTTDVFFEQVCNAANEFATTALSVESAPSFMAERVKERPPWRVK
                     HLDRSFFYVDDEFVAAAGVLERYLSIAAAIQVEKRVRLDPFVVFQVAENDQRLLLSLL
                     QVSRLPGGSGRQFEWIVDEIAAALRFIKSIREEAGL"
     gene            complement(1964..3637)
                     /locus_tag="VNN25_17815"
     CDS             complement(1964..3637)
                     /locus_tag="VNN25_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93444.1"
                     /translation="MSFAAGVGSRGSGVGNEPASSSPTTDTLDATPAVAFALIATVVA
                     VAAIAFAQSYARWLDPIIDTGRDLYIPEQLANGAKLYRDIRYQYPPLAPYLLALITGG
                     IGHSLVSYMAIGIAQSFVIAVSLWMALRRAAGAVPAFATTLSFAALSFCGASTWGANF
                     LFPYSYGATLGMTLLCIALAALIYRRNNVAIAALVLASWFKVEYAIAALLIVAILSVA
                     RRLKLVQAASFVGAMAAIFAAASFYFRDTGWLTENVFASWQHSARAQRFFEVVSGAAM
                     WRDQLTQLALGLAGIMAIALLMRTGRWAAAIPIIIIGILIGNHSFFRAWALLQWLALG
                     WVLVRDRESPLVIFAAFSVASTLRIPLNVSPSWYGFVLTIPVFALAAYTLFCYIPRRN
                     AMVFLWIVPFAANAVGDLWQQHDQYALKTFPIVSSRGTFYDWNADRARTLNALIATVH
                     DGTLAVMPEGVTINYLTRTRTPLTFHTFTPVETADAGIENTILRELASHPPDRVAVVT
                     RDVREYGSRGFGIDYGLRVIELLHTHYRVEREWHGERFDALLLRDRKRR"
     gene            complement(3634..4767)
                     /locus_tag="VNN25_17820"
     CDS             complement(3634..4767)
                     /locus_tag="VNN25_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXH93445.1"
                     /translation="MSTVTELPIAHTDPINARILAISEDRIQGFVRDPIGVIAEQSGV
                     DADTVIERVRAMLRAGTIRRVRQTLMATNLAEGALVAWQYPEKKLDAAFDYMVKDDPF
                     SGHVVIRSTDAATPGSNYRLWTTVKVPQGYSIEKHCAMIGRLTGAEHFRVMPANRIFA
                     LGVGHVRRKGMEPGSKADVPAEAKDTTKVLLSELDWRVMTALKREVEPDEFVRDLWRA
                     RAAEAGVPLETFYDVAESLNARKVVGRFSTFLEHVKPLQTGERVTRFNALFHWAVPHG
                     REIEAGREVGRFHILTHCYWREGGAEFRDVNIMAVAHGTEKEAVLAHKAAIDEHLASI
                     DIPVSYTNVFWGGRSEIKPSEISPFAYVDFCKSLGVDPETMRE"
     gene            5014..>5233
                     /locus_tag="VNN25_17825"
     CDS             5014..>5233
                     /locus_tag="VNN25_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93446.1"
                     /translation="MTFALMLFAERLAPLRRTVEPKLRRVARNLSMGGVSLAIMTLLQ
                     APLLRPVALWILRERVGLLQSVQWPRWIE"
BASE COUNT         1064 a         1589 c         1607 g          973 t
ORIGIN      
        1 ccgtcgagtt cgcgccgatc ccgcgcttcg cgccggagca ctttgccgtg ctgcgggcaa
       61 agcaggtcac gcggcagaag cagtttcaga agggtctggc gcagctcgaa gaggaaggcg
      121 tgatgcaggt gctctcggcg cacgaaggcc ttcgcagtga gccgatcctg gccgttgtcg
      181 gcgagttgca gttcgacgtc atgcagtcgc gcctcgagag cgaatacaac gtcgagacga
      241 ccgtcgtggc gctgccgtac aagctggccc gctgggtcga tcgcgacgtg cacgatgtag
      301 ccacgaagat gaatctcccc agccgctcaa agatcgccac cgacagcgcc ggccgcgccg
      361 tcgtcctctt cagctcgcag tgggagctcg attacgcgga gaaggaaaac cccggcgtga
      421 cgttcctgtc agcggcgtaa ggcgttcgac ccatgaagaa gcgtgccgcc gcccagttaa
      481 tgttcggccc atctgtctcg ccggctggcc agccggcgga cgactgtcgg cccgaacccc
      541 gagttaagtc aggccgttca cctctacaac cccgcctctt cgcgaatgct ctttatgaat
      601 cgcaaggctg cggcgatttc gtcgacgatc cactcaaact gccgtccaga accacccgga
      661 agtctgctga cctgaagaag cgacagcagg agacgctggt cattttcggc gacctggaag
      721 accacgaatg gatctaagcg aacgcgtttt tcgacctgga tggcggcggc tatcgacaga
      781 tagcgctcaa gtacgccagc tgcagcaaca aactcgtcat caacgtagaa gaaggagcga
      841 tcaaggtgct tgactcgcca cggcggccgc tcctttaccc gttccgccat aaagctcgga
      901 gctgattcga ctgaaagtgc agttgtcgca aattcgtttg ccgcattaca cacttgctcg
      961 aaaaatacgt cggtcgtgag aacgtcatcg atgcgaagag cggacatttc gattgcgatg
     1021 ggatattcac gtggaacgaa gattcggacg caggattgtt cgaccgcggt ccagttggcg
     1081 gcgacttcga aatcgaccgc tccgccggcc ggaagagcgc caaagtctac aaacaagccg
     1141 tgttggcggt acaagcggcg tgcttgcaga ggcggaaagt gcatgattac gtttcgcaca
     1201 gtcaccagct tcgccagtgt cgtagtgtag atcactgcgt gatcgggagg caccgactgc
     1261 tcgtcgaatg ccacatggtc agccgcacaa gcgaaaagca aagcgatcaa tgggtcaaac
     1321 gtgaagtcga ccagcgacgt aggcactcca tagtgctgtg ccagtcccgc gaactccgaa
     1381 tcgttttcag gcctcccaat gaggtcgagt tcaattgcat cctctgctgt catgaatttc
     1441 ccaatatggc ctcgcattgc tgctatcgcg cggtcggttg gaaacgtccg acgccatccc
     1501 gtcggccgca aatgccaagg ccgcgcctgg ccccgatata ccggtcgatc gccaaatgta
     1561 gaatcgatga gatcaagatc gctatacgag ccagggatca ttcggcctag aaggttcgct
     1621 gcgaccataa aacaagcagc gtgaccaact gacggagcta agacatccac ggcacgcccg
     1681 tcccgtgagc tttgtgcgga attggcctcg acaacctgtg tcgcggcctc actgacgagc
     1741 tgaagggcgt cggcaggtac ccgcaggcgt tcccccctgg cagagcggat gttgcttcca
     1801 taccacgctg aggcagcggc ctctagcgcc tgagaagatg tgatttgcat gggtattcac
     1861 ctctggggag tgtctattat cggcgccact tctgctcagg cgttttgttc accctgaaca
     1921 aggagtgtgc tgaaacgagg aaaccctacc ctgaggcgat cactcatctt cgctttctgt
     1981 cgcgtagcag caacgcgtcg aaacgctctc cgtgccactc gcgctcgacg cggtagtgcg
     2041 tatgcagcag ctcgatgacg cgcaggccgt aatcgatgcc gaagccgcgc gagccgtact
     2101 cgcgcacgtc gcgcgttacg accgcgacgc ggtcgggtgg gtgtgaggca agctcgcgaa
     2161 ggatcgtgtt ctcgatgccc gcatcggctg tctcgaccgg cgtgaaggtg tggaaagtca
     2221 gcggcgttcg ggtccgggtc aggtagttga tcgtgactcc ctcgggcata acggcgagcg
     2281 tgccgtcatg cacggtcgcg atcagggcgt tgagcgtgcg agcacgatcg gcgttccagt
     2341 cgtagaacgt tccgcgcgac gagacgatcg ggaacgtctt cagcgcatat tgatcgtgct
     2401 gctgccagag atcaccgaca gcgttcgcgg cgaacggcac gatccacaga aacaccatcg
     2461 cgttgcgccg tgggatgtag cagaagagcg tgtatgcggc cagcgcgaac acgggaatcg
     2521 ttagtacgaa gccgtaccac gacggcgaaa cattgagcgg aatgcgcagg gtcgaagcga
     2581 ccgagaacgc cgcgaagatg acgagcggac tctcgcggtc gcgcacgagc acccagccga
     2641 gggcgagcca ttgaaggaga gcccaggcgc ggaagaagga gtggtttccg atgaggatgc
     2701 cgataatgat gatcgggatt gccgccgccc atcgtcccgt ccgcatcaaa agggcgatag
     2761 ccatgattcc cgccagaccg agcgcaagct gcgtcaattg atcgcgccac atcgcggcgc
     2821 cggagacgac ctcgaagaag cgctgcgctc gggcgctgtg ttgccaactg gcgaagacgt
     2881 tttccgtgag ccatccggta tcgcgaaaat agaaggaagc cgcagcgaaa atcgccgcca
     2941 tcgctccgac gaacgatgcg gcctggacga gcttgagccg gcgagcgaca ctcaggatcg
     3001 ccacgatgag cagcgccgcg attgcatact cgaccttaaa ccacgacgcg agcacgagcg
     3061 cggcgattgc gacattgttg cgtctgtaga tgagcgccgc cagcgcgatg cacaacagcg
     3121 tcatccccaa cgtcgcgccg tagctgtacg gaaagagaaa gttcgctccc cacgtcgacg
     3181 ctccgcagaa gctcagcgca gcaaacgaca acgtcgtcgc aaacgccggc acggccccag
     3241 ccgcacgacg cagtgccatc cagagcgaga ccgcgatcac gaacgattgg gcgatcccga
     3301 tagccatgta cgaaaccagc gagtgcccga taccgcccgt gatcagcgcc agcagatacg
     3361 gcgcaagcgg cgggtactga tagcggatgt cgcgatagag cttcgcgccg tttgccaact
     3421 gttccgggat gtagagatcg cgtcccgtgt cgatgatcgg atcgagccag cgtgcgtagg
     3481 actgcgcgaa cgcgattgcc gcgacggcga cgaccgtggc gatgagagcg aatgcaactg
     3541 cgggtgtcgc gtctagggtg tcggttgtcg gagaagaaga agccggttca tttccgaccc
     3601 ccgacccccg actcccgact cccgcggcga aactcactcc cgcatcgtct ccggatcaac
     3661 tcccagcgac ttgcaaaagt cgacatacgc gaacggcgag atctccgacg gcttgatctc
     3721 gctccgtcca ccccagaaga cgttcgtgta gctcaccgga atgtcgatcg atgccaggtg
     3781 ctcgtcgatg gcagccttgt gcgcaagaac ggcttccttc tccgttccgt gtgccaccgc
     3841 catgatgttc acgtcgcgga actcggcgcc gccttcgcgc caatagcagt gcgtgaggat
     3901 gtgaaaccgt cccacttcgc gacccgcttc gatctcgcgg ccgtgcggca cggcccagtg
     3961 aaacagcgcg ttgaagcggg tgacacgctc gcccgtctgc agcggcttga cgtgctcgag
     4021 aaacgtcgag aagcggccga cgactttgcg tgcattgagc gactccgcca catcgtaaaa
     4081 cgtttcgagc ggcactcccg cctcggcggc gcgcgcccgc caaagatcgc ggacgaattc
     4141 gtccggctcg acttcgcgct tcagcgcagt catcacgcgc caatcgagct cgctgagcaa
     4201 aacctttgtc gtgtccttag cttccgccgg gacgtcggct ttgctgcccg gctccattcc
     4261 tttgcggcgg acgtgaccga cgccgagggc gaagatacgg ttcgcgggca tgacgcggaa
     4321 atgttcggcg ccggtgaggc gtccgatcat cgcgcaatgc ttttcgatcg aatacccctg
     4381 cggcaccttc accgtcgtcc agagccggta gttcgatccg ggagtggccg cgtctgtgga
     4441 acggatgacg acgtggccgg agaacggatc gtccttgacc atgtagtcga acgctgcgtc
     4501 cagcttcttt tccgggtact gccaggccac cagcgcacct tctgcgaggt tcgtagccat
     4561 gagggtctgg cgcacgcggc ggatcgtgcc ggcgcgcagc atggcccgga ctcgctcgat
     4621 gaccgtgtcc gcgtcgacgc cggactgttc ggcgatcacg ccgatgggat cgcggacgaa
     4681 tccctgaatg cggtcttcgg agatggcgag gatccgggcg ttgatcggat cggtgtgggc
     4741 aatcggaagc tcggtaacgg tggacataac gcttcgaaga tagcatgttg attcttgaaa
     4801 accggtcacc acagaggcac agagttcaca gagaaacctc cgtgctctct gtgtctctgt
     4861 ggttgccgtt gcttcttcga tcgtgatggg caagccgaag ctctcggaag cgggtaagct
     4921 ggccgtatgt gtaccgcctg aatcaacttt ccggagcagt tccgctccct cacggctaca
     4981 tctcctttca tcatcacggc gaacgtcatc ctcttgacgt ttgcgctcat gctcttcgcg
     5041 gagcgcctcg cgccgcttcg ccggacggtt gagccgaaac tgcgccgtgt ggcgcgcaat
     5101 ctgtcgatgg gtggcgtatc tctggccatc atgacgctcc ttcaagcgcc gctactccgg
     5161 ccggtggctt tgtggatctt gcgggagcga gttggactcc tgcagtcagt gcagtggccc
     5221 cgctggatcg aga
//
LOCUS       DATLVW010000602         5133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_315739, whole genome shotgun sequence.
ACCESSION   DATLVW010000602 DATLVW010000000
VERSION     DATLVW010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315739"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..2182)
                     /locus_tag="VNN25_17830"
     CDS             complement(143..2182)
                     /locus_tag="VNN25_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH93447.1"
                     /translation="MKLGRYEVVRELGKGAMGIVYLAKDPLIGRLVALKTIRPSAHAD
                     DEDTKEFQARFVREAQAAGILNHPSIVTVHDIGVDDPTGVSFIAMEYVEGQNLKEVLS
                     AGRPLSFEQAADIIAQVAEGLDFAHAKGIVHRDVKPANIILLEGNRAKITDFGIAKIT
                     SGANLTTTGQFLGTPNYMAPEQIKGTPVDGRSDIFSLGICLYECLTHRKPFGGDSLTS
                     ISYKIVHEPFPPLHEANPTIPEGFADVVGHCLAKDPVKRYQRGKDMANALRAVIRGER
                     PTRSNEPLFADATVVTRGDREKQMTIEIPFPEAQTAEVPKEPGPQLDTNPAAKPAAAP
                     VAKPVTKTKSAGVDWKKTLNGLVSSTPDLRKLSWKSRVNPILFVAIAAIPLIALAIVL
                     LVLRSKQVAVPVVDKAAEVKVAKERKLRMDANRALHEGRVSDAFARYEELLKLDPRSP
                     AIGSLVQRLSQIRQQDEVGKQQLMAAQQQFDQGLALYNQKKFAEAIPLFEESFHLNPS
                     SDATAQYLKLAQQEDARVKAERASARQAKTTSKTSSQTATTPAVTTAHPGVTTTHAPQ
                     PTSQTSSAPASLTTIINSTVADGYIVVKAGSDEVARENLWAERRFFHTHVPKQVNVTK
                     EFPAKNADLEFWLVVQSLGVSEHRVLRAQNFEPGSNHKLVVTFDPKSKRVDFQFN"
     gene            2377..4473
                     /gene="fusA"
                     /locus_tag="VNN25_17835"
     CDS             2377..4473
                     /gene="fusA"
                     /locus_tag="VNN25_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXH93448.1"
                     /translation="MKVYSGSEIRNVAVVGHNDTGKTSLVTQLLFNAGAITRLGKIDD
                     GTTVTDFDQDEIDRKHSISTAVAFAEWQNSKINFIDTPGFGIFVMEARSAMRVVDSAA
                     IVVSGVTGVEVSTERVWKFAEEFELPRLVVINKMDRERASFTRTLEALQSKFGKNVVP
                     IQLPIGEEKDFAGVIDLVSMKAWKYALDGSGKYETIEIPADMKSEADDWRAQLIEKVA
                     EGDDTLMERFFEQGGLSQEELLDGLKREVSHHQIFPVVLDSAAHNIGGHAILDAIVAL
                     LPPADEVKTIDGKDKKGEPITFDRRAEAFPSALVFKTFSDPFSGRVSLFRVYSGTLQS
                     DHTYWNTTKDHEERVGKLQLIQGKQQVPVSELKAGDIGAVAKLKDVHTGDSLATAQHP
                     IVLEHIHYPEAAISFAVEAKARGDEDKLGVAIGRIIEEDPSIKFGRDEQTKEFLISGQ
                     GQLHVEIVVSKLKKKYGVDVILHPPKVPYRETITKPADAHGRHKKQSGGHGQFADCKI
                     SVEPLSRGADFEFVDEIFGGSIPRQYIPAVEKGIQDARKKGFLAGFPMVDFRVRLKDG
                     QYHDVDSSEMAFKIAGSLAYQQAMEQARATLLEPIMHVDISAPAEYIGDIMGDLNSRR
                     GRVEGVDADGDTQTVKARVPLAEMLTYGSTLRSITQGRGSFHMESSHYEEVPRNLQEK
                     IINEHKKAQAEHQAAH"
     gene            complement(4683..>5133)
                     /locus_tag="VNN25_17840"
     CDS             complement(4683..>5133)
                     /locus_tag="VNN25_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93449.1"
                     /translation="PQVNPVIKRRPVHPMTTGGGPPGEPEPGASLVFPGDADNTPAAR
                     EAVSGNARAEITGTGHGITSTVRIWARRGMMTNVTPGEAMVTVNDLVIGQAKQFSTMD
                     EVYDFPAPGSYTIHLSAPGYKDQQFVITVADDAKLEIARLDVKLEKM"
BASE COUNT          970 a         1603 c         1582 g          978 t
ORIGIN      
        1 tgcatcaagc gcgactccac catcgtgccg cgctgccacg attcgtcgcg cgagacgata
       61 gcgaacgatg ccggcagatt gagaccggag gccgcaggcc ggatttccga cacccgactc
      121 ccagctcccg cgtcccgcgt catcaattga attggaagtc gacccgtttg ctcttcggat
      181 cgaaggtgac caccagcttg tggttgctgc cgggctcgaa gttctgagcg cgcagaacgc
      241 ggtgctcgct cacgccgagg gattgaacga cgagccagaa ctcgaggtcg gcgttcttgg
      301 ccggaaactc cttcgtgacg ttcacctgct tcggtacatg cgtgtgaaag aaacggcgct
      361 cggcccagag attctcccgc gccacctcat cggagccggc tttcacgacg atgtagccgt
      421 ccgcgaccgt gctgttgatg atcgtcgtca acgacgccgg agcacttgac gtctgcgaag
      481 tgggctgcgg cgcgtgcgtc gtggtgacgc ctggatgcgc cgtcgtcacg gccggcgtcg
      541 tcgccgtctg cgacgaggtc ttcgaagtcg tcttcgcctg gcgggccgag gcgcgctcgg
      601 ccttcacgcg ggcatcctcc tgctgcgcca gcttcaaata ctgcgccgtg gcgtcggagc
      661 tcgggttgag atggaacgat tcctcgaaca gcggaatggc ttccgcgaac ttcttctggt
      721 tgtacagcgc cagcccctga tcgaattgct gctgcgccgc catgagctgc tgcttcccga
      781 cctcatcctg ctggcggatc tgcgacaacc gttgcaccag tgatccgatc gccggcgagc
      841 ggggatccag tttcagcaac tcctcgtaac gcgcaaatgc atcgctgacg cggccttcgt
      901 gcagagcgcg atttgcgtcc atccgaagct tccgctcctt cgcgaccttc acctctgctg
      961 ccttgtcgac cacgggaacc gcgacttgct tcgagcgaag gacgagcagg acgatggcga
     1021 gagcgatgag cgggatggcg gcaatggcca cgaagaggat cggattgacg cgcgacttcc
     1081 agctcaactt ccgcaagtcg ggagtgctcg agacgagccc gttcaacgtc tttttccaat
     1141 cgacgccggc ggacttcgtc ttcgtgaccg gctttgctac aggcgctgct gccggcttcg
     1201 ccgccggatt cgtatccaac tgcggacccg gctccttcgg cacctcggcg gtctgagctt
     1261 ccggaaacgg aatctcgatc gtcatctgct tttcgcggtc accgcgtgtg accacggtgg
     1321 cgtcggcgaa aagaggttcg ttcgagcgcg tcgggcgctc gccgcggatc acggcacgca
     1381 gcgcattggc catgtccttg ccgcgctggt atcgcttgac cggatccttt gccaggcagt
     1441 ggccgaccac gtccgcgaag ccctcaggaa tggtcggatt cgcttcatgc agcggcggaa
     1501 acggctcgtg aacgatcttg tatgagatcg acgtcaacga atcgccgccg aacggcttgc
     1561 gatgcgtgag gcattcgtag aggcagatgc cgagcgagaa aatatcgctg cgtccgtcga
     1621 ccggcgtccc tttgatctgc tcgggggcca tgtagttcgg cgtgccgaga aactggccgg
     1681 tcgtcgtcag gttggcgccc gaggtgatct tggcgatgcc gaagtcggtg atcttggcgc
     1741 ggttgccttc gagaagaatg atgttcgccg gcttcacatc acgatgcacg atcccttttg
     1801 cgtgcgcgaa gtccaacccc tcggcgacct gggcgatgat gtcggcggcc tgctcgaacg
     1861 acagcggacg tccggctgac agcacctctt tgaggttctg tccttcgaca tactccatgg
     1921 cgatgaacga aacgccggtg ggatcgtcga cgccgatgtc gtgaaccgtg acgatcgagg
     1981 ggtgattgag gattccggca gcctgcgctt cacggacgaa tcgcgcctgg aactctttgg
     2041 tgtcctcgtc gtcggcatgc gcggaagggc ggatggtctt gagcgccacg aggcgtccga
     2101 tgagcggatc tttggcaagg tagacgattc ccatcgcccc cttgccgagc tcgcgaacga
     2161 cctcataccg gccgagcttc acctggtttc gcttttctcc cgcgtcagct cccgattgcg
     2221 cgggaggttc gtgagccgtc aatcataact ccctgcatcc gtacgatacc gtggatcgcc
     2281 cggcgtgccg cgttaggtgg attccgagtg tggtagcgca ttttaattta tgatctaccc
     2341 ccttttgatg aaatcccgca gcaaaggacg accaacatga aggtttactc gggctctgag
     2401 attcgcaacg tagcggtcgt aggacataac gacaccggaa aaacgtcgct cgtcacgcag
     2461 ctcttgttca acgccggcgc gatcacgcgg ctcggaaaaa tcgacgacgg aacgaccgtc
     2521 accgacttcg atcaggatga gatcgaccgc aagcactcga tctccaccgc cgtggcattc
     2581 gcggagtggc agaactcgaa aatcaacttc atcgacacgc ccggcttcgg aatcttcgtg
     2641 atggaagcgc gctcggcgat gcgcgtcgtc gactcggcgg ccatcgtcgt cagcggcgtg
     2701 accggcgtgg aagtctccac ggagcgggtc tggaagttcg ccgaggagtt cgagctccct
     2761 cggctcgtcg tcatcaacaa aatggaccgc gagcgcgcgt cgttcacgcg cacgctcgaa
     2821 gcgctgcaga gtaagttcgg caagaacgtc gtgccgatcc agcttccgat cggcgaagaa
     2881 aaagacttcg cgggtgtcat cgatctcgtt tcgatgaagg cctggaagta cgcactcgac
     2941 ggctccggaa agtacgagac gatcgagatt ccggcagaca tgaagagcga ggccgacgac
     3001 tggcgcgcgc aattgatcga gaaggtggcc gagggcgacg acacgctgat ggagcgcttc
     3061 ttcgaacagg gcggcctgtc acaggaagag ctgctcgacg gcctgaaacg agaagtgtca
     3121 catcaccaga tctttcccgt ggtcctcgac tcggctgcgc acaacatcgg cggccacgcc
     3181 atcctcgatg ccatcgtggc cctcctgcct cccgcggacg aagtgaaaac gatcgacggc
     3241 aaagacaaga agggtgaacc gatcacgttc gaccgccgcg ccgaggcctt cccgagcgcc
     3301 ctggtcttca aaacgttctc tgatcccttc tccggccgcg tctccctctt ccgcgtttac
     3361 tccggcacgt tgcagtcgga tcacacctac tggaacacga ccaaggatca tgaagagcgc
     3421 gtcggcaagc tgcagctcat ccagggcaaa cagcaggttc cggtttccga gctcaaggcc
     3481 ggcgacatcg gcgcggtggc gaagctgaag gacgtgcaca ccggcgattc gctggcaacg
     3541 gcacagcatc ccatcgtcct cgagcacatt cattatcccg aggccgcgat ctcgtttgcg
     3601 gtggaagcga aggcgcgcgg cgatgaggac aagctcggcg tcgccatcgg ccgcatcatc
     3661 gaggaagatc cctcgatcaa gttcggccgc gacgagcaga ccaaagagtt cctcatctcg
     3721 ggacagggac agcttcacgt cgagatcgtc gtctcgaagc tgaagaaaaa gtacggcgtc
     3781 gacgtgatcc tgcatccgcc gaaagttccg taccgcgaga cgatcaccaa accagccgac
     3841 gcccacgggc gccacaaaaa gcaatcgggc ggccacggac agttcgccga ttgcaagatc
     3901 tcggtcgagc cgctgtcacg cggtgccgat ttcgagttcg tcgacgagat cttcggcggc
     3961 tccatccccc ggcagtacat tccggcggtc gagaaaggga ttcaggacgc gcggaagaag
     4021 ggcttcctgg ccggctttcc gatggtcgac ttccgcgtcc gcctgaagga cggccagtac
     4081 cacgacgtcg attcgtcgga aatggcgttc aagatcgcag gctcgctggc ctatcagcag
     4141 gcgatggagc aggcccgcgc cacgctcctc gaaccgatca tgcacgtcga catcagcgcg
     4201 cctgctgagt acatcggcga catcatgggc gacctcaact cacgccgtgg ccgcgtcgaa
     4261 ggcgtcgatg ccgacggcga tacgcaaacg gtcaaggcac gcgtaccgct tgccgagatg
     4321 ctgacctacg gatcgacgct gcgctccatc acgcagggcc gcggcagctt ccacatggag
     4381 tcatcgcact acgaggaagt cccgcgcaac ctgcaggaaa agatcatcaa cgagcacaag
     4441 aaggcgcagg cggagcacca ggccgcgcac taattgctga cgcctgtggc gtccgcgttg
     4501 tcggttgtcg gttggttgcg ctgcgcgcgt tgtcagttgg ttagctttgc cggttgtcag
     4561 cgacgctcaa gggcttgctg tttcgggctc tccgcggcgg cgcgtagcgc cgcaattgac
     4621 ggacaacgcg cgaagcgcaa tcaactgaca acggacaacg cggccgcctc cggcggccgc
     4681 aatcacatct tctccagctt cacgtccagc cgggcgatct ctaacttcgc gtcgtcggcg
     4741 accgtaatca cgaattgctg gtctttgtag cctggtgcgc tcaggtggat tgtgtagctg
     4801 ccaggcgcgg gaaagtcgta gacctcatcc atcgtgctga actgcttggc ttgaccgatg
     4861 acgagatcgt tgacggtcac catagcctcg cccggagtga cattcgtcat cattccgcgg
     4921 cgtgcccaga tgcgcaccgt agacgtgatg ccgtggcctg tgccggtgat ctccgcgcgg
     4981 gcgttgccgc tgaccgcctc acgcgctgcc ggtgtgttgt ccgcatcgcc gggaaaaacc
     5041 agcgacgcgc ccggctcggg ctcacccggc ggccctccgc cagtcgtcat cgggtgcacg
     5101 gggcgccgtt tgattacagg attcacctgc gga
//
LOCUS       DATLVW010000603        14663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_522599, whole genome shotgun sequence.
ACCESSION   DATLVW010000603 DATLVW010000000
VERSION     DATLVW010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522599"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1284
                     /locus_tag="VNN25_17845"
     CDS             19..1284
                     /locus_tag="VNN25_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH93450.1"
                     /translation="MTNVRWVVCALLFFATTINYIDRQILALIKELLDHDLGWTNEQF
                     GLVNSAFQGAYAVGLLGFGWLVDRYGTKIGYAVSIFAWSLAAASHALVGSVNGFFTAR
                     VALGVSEGGNFPSAIKAVALWFPKRERAFATAIFNAGANVGAIIAPATIPVIAYALGW
                     RWAFIFAGIAGLIWLPFWFWFYETPERRKNVSASELELIRSDADEAGAGGVPMGWIRS
                     LCYRQTWSFIVAKFLTDPIWWFFLIWLPDYFKATRGLNIKKSWVHLVTIYAIVTVLSI
                     AGGWVTGYLSRHGWSVTRARKTGMFLFALCVLPILAATRVANWPAVLIIGLVASAHQA
                     WSANLYTTVSDMFAKKDVGTIIGLGGMAGSIGGILFPWFCGRVLDRYHALGNVTAGYA
                     ILFAICACAYLIAFAIHHLLAPKFEPFEA"
     gene            1281..2723
                     /gene="uxaC"
                     /locus_tag="VNN25_17850"
     CDS             1281..2723
                     /gene="uxaC"
                     /locus_tag="VNN25_17850"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HXH93451.1"
                     /translation="MTPFIHEDFLLETDVARDLYHRFVADLPIVDYHCHLPVSQIAGD
                     HRFRSLTEIWLDGDHYKWRAMRANGVAERFCTGDAADQEKFEVWARTVPATLRNPLYH
                     WTHLELKRPFGVTELLSERTARSIYTHCNELLQRPDFSTQGLLRQFRVAVVCTTDDPA
                     DSLEHHHALAQRVDPDTSVVPTWRPDAALAIEDSPSFNGWLDRLEAASGVSIATVDQL
                     FDALDQRHAAFHDAGCRASDHGLEQIYADSWSDTDVAATFGKLRSGKPVDADEGRRYK
                     SALLHRFALMDHARGWVQQYHLGALRNTNSRMRRLLGADSGLDSVGDFDMARSLAHFL
                     DRLDETSQLAKTILYNLNPRDNELFATMIGNFQDGSVPGKMQYGSAWWFLDQKEGMEA
                     QIRALSNMGLFSRFVGMVTDSRSFLSYSRHEYFRRVVCNLLGDDVRRGLLPDDRNLLG
                     ALAQDVSFFNARNYLGLDLGRLGQNVARAT"
     gene            2720..3484
                     /gene="kduD"
                     /locus_tag="VNN25_17855"
     CDS             2720..3484
                     /gene="kduD"
                     /locus_tag="VNN25_17855"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HXH93452.1"
                     /translation="MNVLDSFRLDGQVAIVTGASRGLGAAMALALAEAGADMALVARG
                     DLSESQGNIERLGRRSIAINADLEDRAAPEAILDATQRAFGRADILVNNAGIIRRAAL
                     LDYTDEDWDAVIEVNLRSAFALSRAFARALVARNSGGKIINIASMLSFQGGVRVAAYT
                     AAKSGLLGLTRAMANELAPLGINVNAIAPGYMATDNTQALRDDPTRSREILARIPAGR
                     WGAPDDLKGAVVFLASPAAAYVHGTTLAVDGGWLSR"
     gene            3929..6910
                     /locus_tag="VNN25_17860"
     CDS             3929..6910
                     /locus_tag="VNN25_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH93453.1"
                     /translation="MPTTSEPILRSIKAVSARPGDAESLHVAGVTRLLLGRVEKAIEL
                     LEQASECDRADPAILNDLAVAHLERASREGDGIDLIDAFELADRSLRLNPTSEEAAFD
                     RALAIERLGLRTEAIAAWRSVRVPTEWTAEAAEHIAHLSTPTRRALWDDLVGQFEGAA
                     AKGDVNTVAAIVKLYPQEVRRHFEENLCGRWALAVWEGKSDEGDRLLRVLRTVAAALQ
                     STTGNELPARTVAAIDQAKHSDYWKQLVRGHLVYQQAIRTYRDDAFTRAEPDFAESEK
                     LLRETNSPFALLALFYRASSRSYENDITSCLRLLDQTERALLAAKLTRSPAYGNVLWV
                     RGRSLLKRGLPHASLECFKSALAVFTPTGELESQAALHDLVAYNDQFLGEDDEAWKQS
                     VMAVNLVENVGATRRERVILHGLADFSMNRRRTHTALLYSSALVRLSVAANDVGDQAR
                     ALLDRGRAFHAAANWQAGDADLRLARALARQVSDRGMRDELYADLSAAEADSGTSSSP
                     DIALARFDRAIDTATRVEKETLLPRLWAARARTARSLGLRQEAERSLRQGIAVIERHR
                     WTMTDRDLQIAFAEQWYIAFDEMIDLLVDQGRYAEALSFAERSRLSVLFDQLNGARPP
                     IEFSPMSARRIPEGDAVVEYAVLDNSIVVWVVRRDSLLCRRLPVGGSEIERLVATARA
                     SIDQYSDDDASARRDLGHLYDAIVGPVVSELRRAERLVIVPDKALQGVPFAALYDRQH
                     QRWLIEDRIVAIAPSLNLFLTAITRDAALACEQQERILTLGDPAFDRRRFPMLPRLDA
                     ANAEARRIARLYPKAVVLTGSEATPSRLKKEAQGCPIVHLATHAISNPRLPSWSALLL
                     APEPDTLATGVLYAHDIDRRTFSRTRLVVLAGCETASAMRTRYEGSLGLARSFLAAGV
                     PSVVSTLWRIDDETSQNLLLRFHQRLRAGDDSPNALRAAQLDQLHSSDKALRRVATWA
                     GFQVIGGVFRVCPSHTTKGAG"
     gene            6910..7935
                     /locus_tag="VNN25_17865"
     CDS             6910..7935
                     /locus_tag="VNN25_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93454.1"
                     /translation="MLNAALFTTKVMLTGVLLLVPSAKNNHLVRVIAPNGSIQHLGLP
                     THVSYVMFNIDNWDPSGRKPDFFVSRPDPNDPTHMANFGVCEIDKEYLEATGSFTQSP
                     LCQPTPSSFALAPCNLTARRTDQAFPSVINENLVTNQDSLQWLFDFSRANPDIASHIS
                     DDVLQVKPATGKASLRMDLKFGEMRVLPNSFDLHYAYRLTATGTCQALARAVVVNLEN
                     SQDPSTLTLTSTSFSGSVTQLPLKLSASTGDLAVLIGNEPLESIPMSVDFLLGDTSSQ
                     HHEDTTDHQQRIRDLLSGLSTLITPAEIPAGACQNGHAPRIKTGAAGGGGTCNPGGTP
                     PTDPSGG"
     gene            8136..8621
                     /locus_tag="VNN25_17870"
     CDS             8136..8621
                     /locus_tag="VNN25_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93455.1"
                     /translation="MVTPIPPLGTNWCWAASGQMAMNALEMQVQHLQCEQANQRFGFN
                     DCCKLPRPGRCEQPGGGIPPLREFSSVPSQGALTSDQIKDEICVQQRPFLVVTPGTGG
                     GGHMVIAIDYAADDQGELLVVYNPWMKGKVDQGYITLDDYKNTHTTNDHDFYKIKKGP
                     Q"
     gene            8618..9349
                     /locus_tag="VNN25_17875"
     CDS             8618..9349
                     /locus_tag="VNN25_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93456.1"
                     /translation="MIRIAAVAMLLVHVPLAAANTAANFIQQARKSVASSSEAAQAAR
                     RRLATLIERDPDTRSGVAVETTKGNPNYVLDNANPGVGNPNPGVGNPNPGVLPIYIVP
                     LDRLREFDDHTVALDILIPANRVYYLVSYPIDSLPPGHFVTLSSVELMNTSVGWKTAI
                     MGGGTYAESVWRTLQNVQQDAILVQIPALALNFIGFSRDGQHLDLSLIAGDSDSPIPE
                     RKPYRAEAVLLKLVPLAKRHNGLIN"
     gene            complement(9522..9597)
                     /locus_tag="VNN25_17880"
     tRNA            complement(9522..9597)
                     /locus_tag="VNN25_17880"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9562..9564),aa:Thr,seq:cgt)
     gene            complement(9848..10537)
                     /locus_tag="VNN25_17885"
     CDS             complement(9848..10537)
                     /locus_tag="VNN25_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93457.1"
                     /translation="MKRSLAAFALTLLVTPLMAAKDEPNRAARVLAETPGLITLADDD
                     GGRVEVFRLGGAPRERSFHGGAVIKEPMIEMIFLGDWTAPSAAARKSELQRQVAQIST
                     SESFQATARYGVRTTGLLLSSRDLAASGKVNDLRIQAKLDAGMTDGSLPLRDENVVHL
                     IFLAPALQSVLSSSLAGRDYQSYHSHVQLQDINVRYVVIPYDQDLARMFNAAQQSFLR
                     AVVNPDGDAWY"
     gene            complement(10552..12510)
                     /locus_tag="VNN25_17890"
     CDS             complement(10552..12510)
                     /locus_tag="VNN25_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH93458.1"
                     /translation="MRKFAAPLFFILVLVSVPTQILAQCGVERWSVKTGTDADVGLIN
                     LSSSTAQTITTMRGWPTPSSIPANNRISPYETTQWVLNATLTQYKLESDSDYHLVLSD
                     ASGNTLIAEIPSPSCVGSGSPFGPGIQNARNEFDARYTATGSFQTANIPVQVKGVGMF
                     DFLHGQTGVAPNGIEIHAVLDIIFNPTGADFTIGSSPGSISVVQGTSGTSTISTTKSG
                     TFNSAISLSASGLPSGATASFSPTSIAAPGTGSSTATITTSPSTPAGTYTVTVTGTGG
                     GVTHTTTITLTVTAAGGGVTTLTNGVAVTGLSGAVGSMQFFKITVPAGQTSLAIPISG
                     GTGDADLYVKLGAQPTTTTYDYRSIVSGNTDSVTVTNPAAGDWYIGLYGYAAYASVTL
                     KATYTGGSGDTTAPTTSITAPTAGAIVSGTTTVSASASDNVGVTKVEFYLDGVLQSTD
                     TTSPYSWSWNTTTASNASHTLTSKAYDAAGNVGTSAGVAVTVSNATAQQLLGNTGFET
                     GTASPWVAASGVVTNSTSEAAHSGSWKAWMDGYGAAHTDTLYQQVAIPSTVTTATLTF
                     WLHIDTAETTTTTAYDTLKLQIRNSSGTVLATLVTYSNLNANTGFAQKTFDLSAYKGQ
                     TIQVYLVGVEDASLQTSFVVDDFALNVQ"
     gene            12714..13034
                     /locus_tag="VNN25_17895"
     CDS             12714..13034
                     /locus_tag="VNN25_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93459.1"
                     /translation="MTRKLLLTLLLTSAVSGCVFVPSVMWNRAETNRQHINAVHVGQS
                     LAEVRQIMMKDPEKREMRSRFDGKTVEFWSYVTDYGRRLDSTITFIDGHVQEIRTTPW
                     MEKD"
     gene            13131..>14663
                     /locus_tag="VNN25_17900"
     CDS             13131..>14663
                     /locus_tag="VNN25_17900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH93460.1"
                     /translation="MGVGGRRSEAEDLPTANAGSPPPGLPRSERIALIAIAAVTLAMR
                     IVAYFHYRFDSDEPQHLHVAWGWTAGLLQYRDVFDNHAPLFHMATAPLLRLLGERADI
                     LFWMRGPMVLLWLVVVACTYVVARRLYDVRIAIWSIVLLNVLPPFFLKSIEFRTDNLW
                     NAFWMLAIVVFLLVRRSAGTFFLAGLLMGCAFSVSLKTLLLLLTLAAAGAITWFMRRR
                     ARGAGVRAGLVAVFVLGFLIVPALIAAYFIARGAWPDLVYGALRFNGLVAGHRPPLVV
                     WIRRLCYIPMMYVVFRQAWRYRGRTTDARYFFAIATGIFFATLFSFWILISPRDFLPF
                     MPFLSIFFVAALARRKFFLEAIIVTTIVLLTGVGYYSEWLLNRTREFITMENQLLNVS
                     RPGEMVMDYKGELIYRRRPYYFILEFITRHAIERGLIADTVPEDLVRTRCHIAQADGT
                     QWPIRARVFMNANFIDIGRLRVSGQWLQTNGEFTIAIPGDYVIVNKNGEAVGALDGTP
                     YRG"
BASE COUNT         2747 a         4580 c         4428 g         2908 t
ORIGIN      
        1 gcggctgccg aggagcgcat gaccaacgtc cgttgggtcg tttgcgccct gctgttcttc
       61 gccaccacga tcaactacat cgaccgccag atcctcgcgc tcatcaaaga acttctcgac
      121 catgacctgg gatggacgaa cgaacagttc ggcctcgtca actccgcgtt tcaaggcgca
      181 tacgctgtcg gacttctcgg cttcggctgg ctcgtcgacc gctacggcac aaagatcggc
      241 tacgcagtct cgatctttgc ctggagtctg gccgctgcgt cacacgctct ggtaggttcc
      301 gtcaacggat tcttcaccgc acgcgtagcg ctcggcgtga gcgagggtgg caactttccc
      361 tcggccatca aagcagtagc gctctggttc ccgaagcgtg agcgtgcctt cgccaccgcc
      421 atcttcaacg ccggcgcgaa cgtcggcgcc atcatcgccc ccgccaccat tcccgtcatc
      481 gcctacgccc tcggctggcg atgggcattc atcttcgcag gcattgccgg cttgatctgg
      541 ctgccattct ggttctggtt ttacgagacc cccgaacggc gaaaaaatgt ttccgcctcg
      601 gagctggaac tgattcgcag cgatgccgac gaagccggcg ctggcggcgt accgatgggc
      661 tggattcgct cgttgtgcta ccggcaaacg tggtccttca tcgtggcgaa gtttctgacc
      721 gatccgatct ggtggttctt cctgatctgg ctccccgact acttcaaagc cacgcgaggc
      781 cttaacatca agaaaagctg ggtgcacctc gtgacgatct acgcgatcgt aacggtgctc
      841 agcatcgccg gcggctgggt gaccggctac ctgagtcgtc acggatggag cgtcacacgc
      901 gcacgcaaga cggggatgtt cctctttgcg ctctgcgtgc tgccgatcct ggcggcgaca
      961 cgcgtggcca actggcccgc ggtgctcatc atcggcctgg tggcttccgc gcaccaagca
     1021 tggtcagcaa atctctacac caccgtctcc gacatgttcg ccaagaagga tgtcggcacg
     1081 atcatcggtc tcggcggcat ggccggatcg atcggaggca ttctctttcc ctggttctgc
     1141 ggacgcgtgc tcgaccgcta tcacgcactc ggcaacgtca ccgccggata cgccatcctg
     1201 tttgccatct gcgcgtgtgc gtatctgatc gctttcgcca tccaccatct tctcgcgccg
     1261 aagttcgagc cattcgaagc atgactccat tcatccacga agacttcctg ctggagacgg
     1321 acgtcgcccg cgatctctat caccgcttcg tggctgacct tccgatcgtc gactatcact
     1381 gccacctgcc ggtgtcgcag atcgcaggcg atcatcgctt ccgatcactg accgaaatct
     1441 ggctcgacgg cgaccactac aagtggcggg ccatgcgcgc caacggtgtt gccgaacgct
     1501 tctgcacggg cgacgcggcc gatcaggaaa agttcgaagt gtgggctcgc accgttcccg
     1561 ccacgctgcg caatccgctg taccactgga cgcatctgga gttgaagcgg ccgttcggtg
     1621 tgaccgagct gctgtccgaa cgaactgctc gctcgatcta cactcactgc aacgagttgc
     1681 tgcaacgtcc ggacttctcg acgcagggac tgctgcgcca gtttcgcgtg gccgtcgtct
     1741 gcacaaccga cgatccggcc gactcgctcg agcaccatca cgcgctcgcg caacgcgtcg
     1801 atcccgatac aagcgtcgtc ccgacctggc gccccgacgc agcattggcg atcgaagatt
     1861 caccgtcgtt caacggatgg ctcgatcggc tcgaggccgc gagcggcgtg tcgattgcga
     1921 cagtcgacca gttgttcgac gcactcgatc aacgccacgc cgcgtttcac gacgccggct
     1981 gccgcgcctc ggaccatggt ctcgaacaga tctacgccga cagttggagc gatacggacg
     2041 tcgcagctac gttcggcaaa cttcgatccg gcaagcctgt tgatgcggac gaaggccgcc
     2101 ggtacaagtc cgcgctgctc catcgtttcg cgttaatgga tcacgcacgc ggctgggttc
     2161 agcagtacca cctcggcgcg ctgcgtaaca ccaattcgcg gatgcgccgc ctgctcggcg
     2221 ccgactccgg cctcgactcg gtcggcgact tcgacatggc gcgttccctc gcccatttcc
     2281 tcgatcgact cgatgaaacg agccagctcg cgaagaccat cctctacaac ctcaacccgc
     2341 gcgacaacga gctctttgcc acgatgatcg gtaacttcca ggacggctca gtccccggca
     2401 agatgcaata tggctccgcg tggtggttcc tcgatcagaa agaaggcatg gaagcgcaga
     2461 tccgcgcact gtcgaacatg ggcctcttct cgcgcttcgt cggcatggtc accgactcac
     2521 gcagttttct gtcctactcg cgccacgaat acttccgccg cgtcgtctgc aacctcctcg
     2581 gcgatgacgt tcgccgcggg ctccttcccg atgaccgaaa cctgctgggc gcactggccc
     2641 aggatgtctc gttctttaac gcccgcaact accttggcct cgatctcggc cgcctcggcc
     2701 aaaacgtagc gcgcgccaca tgaacgtcct cgactcgttc cgcctcgacg ggcaagtcgc
     2761 aatcgtcacc ggcgcctcgc gcggcctcgg cgccgcaatg gcgctggcgc tcgcagaagc
     2821 gggggctgac atggcgctcg tcgcgcgcgg cgatctgtcc gaatcgcagg gaaacatcga
     2881 acgcctcggc cgcaggtcga ttgccatcaa cgcggatctc gaagatcgag cagcccccga
     2941 agccattctc gatgccacgc aacgcgcgtt cgggcgcgcg gacatcctcg tcaacaacgc
     3001 cggcatcatc cgccgcgctg cgcttctcga ctacaccgac gaagattggg atgcagtcat
     3061 cgaggtgaac ctccgctccg cattcgcgct cagccgtgcg ttcgcgcgcg cactcgtcgc
     3121 gcggaacagc ggcggcaaga tcatcaacat cgcctcgatg ctgtcgttcc agggtggcgt
     3181 tcgcgtcgcc gcctacaccg ccgcgaagag cggactactc ggattgaccc gtgccatggc
     3241 caacgaactg gccccgctcg gcatcaacgt aaacgccatc gctcccggct acatggcgac
     3301 cgacaacacc caagcccttc gcgacgaccc cacgcgcagc cgcgagatcc tggcccgcat
     3361 tcccgccggc cggtggggcg cgccagacga tctgaaaggc gccgtcgtct tcctcgcctc
     3421 cccggccgcc gcatacgttc acggcacaac gctcgcggtc gatggagggt ggctgtcccg
     3481 ctgagtggtg cgttcgcaga gaactgtggc ggcataacgt catcgacgat gtctgggcgc
     3541 ccaaagataa gcctgcgatc ctctcccttt cgtctcggcg cgcgcaaccc atgagcacgc
     3601 cttgcgtaat cgcatagaat gttcggcatc ccatttactg cgtaataaac tccacggaaa
     3661 accgatgaca actcggcaag tgatactcat cggttcggat gccaccccac ggcgtgcggc
     3721 aaagcggagc cggtggcaaa cggcacgcct gatcgtgctc ccgacgctcg ccgcagccgc
     3781 gttggccgcg gttgcatggc tcgcccacga caagcggttt gagcggcact cggagatgga
     3841 gttgctcgcg gatcaagttg gcaccctgcc gtaccgagtg gttgatgggc gactctctgg
     3901 tttcaagtac aggccgaggc gagcaacggt gccaacgaca tccgaaccta tcctaagatc
     3961 catcaaagct gtctcagcca ggccaggcga tgcggagtca ctccatgtag cgggagtaac
     4021 acgcctgctt cttgggcgtg ttgaaaaagc catcgagcta ctggagcagg cgtcggagtg
     4081 tgatcgggcc gatccggcaa ttctcaacga tctcgcggtc gctcatctgg agcgggcatc
     4141 tcgcgaaggc gacggaatcg acttaattga cgcgtttgag ctcgcggacc gttccctgcg
     4201 gctcaatccg acctccgaag aagcggcatt cgatcgcgca ttggcgattg agcggctcgg
     4261 cttgcgaacg gaggccatcg ccgcgtggcg ctccgtccgc gttccgactg agtggacggc
     4321 ggaggcggca gagcacatcg cacatctgtc gacaccaacg cggcgagcat tgtgggacga
     4381 cctggttggg caattcgaag gtgccgcggc gaagggcgac gtgaatacag tggcggcgat
     4441 cgtcaagctc tacccgcaag aagtgcgacg tcatttcgag gagaacctct gcggtcgttg
     4501 ggctctggcg gtctgggaag gaaaaagcga cgaaggcgac aggcttcttc gtgtactgcg
     4561 gaccgtggca gcggctcttc aatcgacgac gggcaacgaa ttgccggccc gcaccgtcgc
     4621 cgcgatcgat caggcgaagc actccgatta ttggaagcag cttgttcgcg gtcatcttgt
     4681 ctatcagcaa gcgattcgca catacagaga cgatgcgttc acgcgcgccg agcccgattt
     4741 cgcagagagc gagaagctcc tcagggagac taactcaccg tttgcattgc tggccctatt
     4801 ctaccgggcc agttcgcgct cttatgagaa cgacatcact tcgtgcttgc gactcctcga
     4861 tcagaccgaa cgcgcattac ttgccgcaaa gctcactcgt tcacctgctt atggcaatgt
     4921 actctgggtg cgcggccgct cgcttctgaa gcgaggactt ccacatgcct cgctggaatg
     4981 cttcaagtcc gctctcgcgg tcttcacgcc tactggagag ctggagagcc aggccgccct
     5041 gcacgatctc gtggcataca acgaccaatt tctcggcgaa gacgacgagg cgtggaagca
     5101 gtccgtcatg gcggtcaatc tggtcgagaa cgtgggggcc acgcggcgcg agcgcgtcat
     5161 cttgcacgga ctagccgatt tctccatgaa ccggcggcgc acacacacgg cgctgctata
     5221 ttcctcggcc ctcgtccggc tgtcggtcgc ggcgaacgat gttggcgacc aggctcgagc
     5281 gttgctcgat cgaggcagag ctttccacgc cgcggcgaac tggcaggccg gcgacgctga
     5341 ccttcggctc gcccgagccc tcgccaggca ggtgtcggat cgcggcatgc gcgatgagct
     5401 ctacgccgac ctctccgctg cggaagcaga ttcgggaacg tcatcttcgc cggacatagc
     5461 tctggcgcgt ttcgatcgag ccatcgatac ggctaccagg gtcgaaaaag agacgctgtt
     5521 accccgcctt tgggcagcgc gagcccgcac cgcccgctca cttggcctcc ggcaagaggc
     5581 ggagcgcagc cttcgccagg gtattgccgt catcgaacgc caccgctgga cgatgactga
     5641 tcgcgatctt cagatcgctt ttgcggagca gtggtacatc gcgttcgacg aaatgatcga
     5701 cctcctggtc gatcagggcc ggtacgccga ggcgctctcg tttgccgagc ggagccgatt
     5761 gagcgtgctc tttgaccaac tcaacggcgc caggccaccg atcgaattct cgccgatgtc
     5821 ggcgcggagg attccggaag gcgacgccgt cgtcgagtac gcagtgctcg acaactcgat
     5881 tgtcgtctgg gtcgtccggc gcgacagctt gttatgcagg cgtttacccg tcggaggaag
     5941 cgagatagaa agactcgtcg ccaccgcgcg agcgagcatc gatcaatatt ctgacgacga
     6001 cgcatccgcg cggcgcgatc ttggccacct gtacgacgcg atcgtcggcc cagtggtgag
     6061 tgagctcaga cgggcagaga ggctggttat tgttccggat aaggcgctcc agggcgtgcc
     6121 gttcgccgcg ttatacgacc gtcagcatca acgctggttg atcgaggatc ggatcgtcgc
     6181 catagctccg agcttgaacc tctttctgac cgccattacg cgcgacgctg ccctcgcctg
     6241 cgagcagcaa gagcgcatcc tgaccttggg cgatccagca ttcgaccgtc gtcgatttcc
     6301 aatgcttcca cgcctcgacg ctgcgaacgc ggaagccagg cgtatcgcgc ggttatatcc
     6361 gaaggcagta gttctcaccg gctcggaggc gacacctagt cgcctgaaga aagaagcgca
     6421 aggctgcccg atcgtccacc tcgcaacaca tgcgatttcg aacccacggc tgccgtcatg
     6481 gtcagctctt cttctcgccc ctgagccgga tacgttggca accggagtcc tttacgcgca
     6541 cgacatcgac cgccgcacat tcagccggac gcgattggtc gtcctcgccg gctgtgagac
     6601 agcatcggcg atgaggacac ggtacgaggg ctcgctcggg ctggcacgaa gctttctggc
     6661 cgcaggcgtt cccagcgtcg tctccacgct gtggcggatc gacgacgaaa ccagccagaa
     6721 cctccttttg cggtttcacc agcggcttcg ggccggtgac gattcgccca acgctttacg
     6781 agcggcgcaa ctggatcagc tacacagttc agacaaagcc ctgcggcgcg tcgccacatg
     6841 ggctggtttt caggtgatcg gtggagtttt ccgagtctgt ccgtcccaca ctaccaaagg
     6901 agcagggtaa tgctaaatgc cgcgctgttc acgacgaagg tcatgctgac aggagtactc
     6961 ctgttggttc cgtcggcgaa aaacaatcat ctcgtgcgcg tgattgcgcc aaatggaagc
     7021 atccagcacc tcggactacc gacgcacgtg tcctatgtga tgttcaacat cgataattgg
     7081 gacccatcgg gcaggaagcc cgatttcttc gtcagccgcc cggaccccaa cgatccaact
     7141 cacatggcga acttcggcgt ttgcgagatc gacaaggagt acctcgaagc aacgggctcg
     7201 ttcacgcaat caccgttgtg tcagcccacc ccgtcgtctt tcgctttggc gccctgcaac
     7261 ctgacagccc ggagaacgga ccaggcgttt ccttccgtga tcaatgagaa cctcgtcacc
     7321 aatcaagact cgctgcagtg gttgttcgac ttcagccggg ccaaccccga tatcgcttcc
     7381 catatctccg acgacgtgct gcaggtgaaa ccggcaacag gcaaggcatc gctgcggatg
     7441 gacctcaagt tcggcgagat gagggttcta cccaatagct tcgacctgca ctatgcctac
     7501 cggctcacag caacagggac atgccaggcg ttggcgaggg ctgtcgtcgt gaacctggaa
     7561 aacagccaag atccatccac tttaacgttg acctcgacat cgttcagcgg atcggtcacc
     7621 caactcccgc taaagctctc ggcatcaaca ggagatcttg ccgtcctgat aggaaatgaa
     7681 ccgctcgaaa gcatccccat gtcggtcgat ttcctcctag gcgatacgtc ctcacaacat
     7741 cacgaggata cgaccgacca tcagcagagg atcagggatc tcctgtctgg actatcgacg
     7801 ctgatcacac cggcagagat tccggctggc gcctgccaga acgggcatgc tccgagaatc
     7861 aagaccggcg ccgcgggcgg cggcggaact tgcaatcccg gcggcactcc cccaactgac
     7921 ccaagcggag gctagtcgtc aatatgatga ccgcactacg aatttccatt cgcttcgcgc
     7981 ttgccgctct tactggagtg gtcttgtcga cactcggtgc ctgtaagagt ggggcgccga
     8041 gggttcccac tctggctcac gcagccgtgc acaacccgac agtgccaccg ccgccgctaa
     8101 gcaggtgtgg ctccgccgcc gggaatgtac ggcaaatggt gacgccgatt cctccattgg
     8161 ggaccaattg gtgttgggcc gccagcgggc agatggcgat gaacgctctg gagatgcaag
     8221 tccagcatct tcagtgcgag caggccaacc aacgttttgg ttttaatgac tgctgcaagc
     8281 tgccgcgtcc gggaaggtgt gagcagccgg gtggcggcat tcctccgttg agggagttct
     8341 cctcggtgcc ttcccaaggt gctttgactt ctgaccagat caaagacgag atctgtgtac
     8401 aacagcgtcc attcctggtc gttacgccgg gcacaggcgg aggcggacac atggtgatcg
     8461 ctattgacta tgcggctgac gaccagggag agctactcgt cgtgtacaac ccctggatga
     8521 aggggaaggt cgatcagggc tatatcactc tcgatgacta caagaacacc cacaccacga
     8581 acgatcatga cttctacaag atcaagaagg ggccgcaatg atcagaattg ctgcagtggc
     8641 catgctgctg gttcatgttc cgcttgccgc ggccaacacc gccgcgaact tcatccagca
     8701 ggccagaaag tccgtggcct ctagcagtga ggccgcccag gcggcgcggc ggcgcctggc
     8761 cacacttatc gagcgcgacc ccgatactcg ctcaggcgtt gccgtggaaa cgacaaaggg
     8821 caaccccaac tatgtattgg acaacgccaa cccgggagtg ggcaacccca accccggagt
     8881 gggaaacccg aacccggggg tgcttccaat ctacatcgtt ccactcgatc gccttcggga
     8941 gttcgacgac cacacggtgg ccctggatat cctgatacca gcgaaccgcg tctactacct
     9001 ggtttcctat cccatcgatt cacttccgcc aggtcacttc gttaccctgt cctccgtgga
     9061 gctgatgaac acgtccgtcg gatggaagac ggcgatcatg ggcggtggaa cgtatgcgga
     9121 gagcgtttgg aggacgctgc agaacgtcca gcaagatgcc atcttggtac agataccagc
     9181 gttggcgctg aacttcattg ggttctcacg agatggccag catcttgatc tctccctgat
     9241 tgccggtgat agcgacagcc caatccccga gcggaagccg taccgtgccg aagctgtgct
     9301 cctgaaactc gtcccattgg cgaagcggca caacgggctg atcaactgac ggccggcttc
     9361 gcttgctgac gttgaccgcg gcgtccgacc gagcgcttcg caatagatga agagcaactt
     9421 cgaagcacgg aacgataccc cgacgaggca gaacagcggt tcgcgatccg cccttagaac
     9481 gaagaagcca ctgtttccag tggcttcggt tcggtttcgc ttggagccgt tgagcggatt
     9541 tgaaccgctg acctgctgat tacgaatcag ctgctctacc aactgagcta caacggcttg
     9601 agggatgcag cgggtgattc tactgaggtg agtcggtgct gtcgagcggt ccggagaacg
     9661 cggggtggga gcggcgcatt ccggagcgaa ggtgggggtc aggtctaaag tctaaagtgc
     9721 cgcattggcc tgatcgcatt cgcgacgatc gtcctcggca ctttagactt tagacctgac
     9781 gcgtcaacat cgcaaaaaag agtcccgcgg gctgcgcggg actctcttcg tcgaatcgct
     9841 ttcgggttta gtaccaggcg tcgccgtccg ggttcacgac ggcgcgcagg aaactctgct
     9901 gcgcggcatt gaacatccgc gccagatcct gatcgtacgg gatgacgacg tagcggacgt
     9961 tgatgtcctg aagctggacg tgggagtgat acgactggta atcgcgtccg gccaggctcg
    10021 aggagaggac cgattgcaac gccggcgcga ggaaaatgag atggaccaca ttttcgtcgc
    10081 gcagcggaag cgaaccgtca gtcattcctg cgtcgagctt cgcctggatg cggagatcgt
    10141 tcaccttgcc gctggccgca aggtcgcggc tactgagaag gagtcctgtc gtgcgcacac
    10201 cgtagcgggc ggtcgcctgg aacgattccg atgtgctgat ttgagctacc tgacgctgca
    10261 gctcggattt tcgcgcggcc gccgacggag ctgtccagtc gccgagaaaa atcatctcga
    10321 tcatcggttc tttgatcacg gcaccgccgt ggaacgaacg ttcacgaggc gcgccgccga
    10381 ggcgaaacac ctcgacgcgg ccgccatcgt catccgcgag cgtgatcaaa cccggagtct
    10441 cggccagcac gcgcgcggcg cggttcggct cgtccttggc cgccatcagc ggggtcacca
    10501 ggagcgtgag ggcaaacgca gcaagtgatc tcttcatttt catctccctg cttactgaac
    10561 gttgagtgcg aaatcgtcga cgacgaagga agtttgaagt gaggcgtcct cgacaccgac
    10621 aaggtagacc tgaatggtct gtcccttgta ggccgagagg tcgaaggtct tctgagcaaa
    10681 gccggtgttg gcattgagat tcgagtacgt taccagcgtc gccagcacag ttcccgagga
    10741 gtttcggatc tggagcttca gcgtgtcgta cgccgtagtc gtggtggttt ccgcggtgtc
    10801 gatatggagc cagaacgtca gcgtggccgt tgtgaccgtc gacggaatcg ccacttgctg
    10861 atagagggtg tctgtgtggg cagctccata cccatccatc cacgctttcc acgagccgga
    10921 atgcgccgcc tcggacgtcg aattcgtgac gacgccgctt gccgcaaccc acggcgatgc
    10981 ggtgcccgtc tcgaatccgg tgttgccgag gagttgctgc gccgttgcgt tgctgaccgt
    11041 cactgctacg ccggccgagg tgccgacatt gcccgcggcg tcgtacgcct tcgacgtgag
    11101 ggtgtgcgat gcgttgctcg ccgtggtcgt gttccagctc catgagtacg gcgaggtcgt
    11161 atcggtcgat tgcagaacgc cgtcgagata gaactcaacc ttcgtcacgc cgacgttgtc
    11221 ggaggcggac gcggataccg tcgtcgtgcc actgacgatg gcaccggccg tgggcgccgt
    11281 gatcgacgtc gtaggcgcgg tcgtgtcgcc gctgccaccg gtgtacgtcg ccttcaaagt
    11341 cacgctcgca tacgcggcgt agccgtaaag accgatgtac cagtcgccgg ccgcggggtt
    11401 ggtcacggtc acgctatccg tgttgccgga aacgatcgag cggtagtcgt aggtggtcgt
    11461 tgtcggctgc gcaccgagct tgacgtagag atccgcgtca ccggtgccgc cgctgatcgg
    11521 aatggcgagg ctggtctgcc cggccggcac cgtgatcttg aagaattgca tgctgcccac
    11581 ggcgcccgaa aggccggtta cggccacgcc attcgtgagg gttgtgactc ctcccccggc
    11641 tgccgtcacc gtgagcgtga tggttgtcgt gtgcgtgaca ccgcctcccg tgccggtgac
    11701 ggtgaccgtg taggttcctg ctggtgtcga tggactggtc gtaatcgtcg ccgtcgagct
    11761 tcccgttccg ggtgcggcga tcgaggtcgg gctgaacgat gcagtcgcgc cgctcggtaa
    11821 tccggatgcg gacaacgaga ttgcggagtt gaacgtgccg gatttcgtgg tggagatcgt
    11881 cgacgtaccg gacgttccct gcacgacgct gatcgagccc ggggaggacc cgatcgtgaa
    11941 gtcggcgccg gttggattga agatgatgtc gagcaccgcg tgaatctcga tgccgttcgg
    12001 cgcgacgccc gtctggccgt gaaggaagtc gaacatcccg acgcccttga cttgcacggg
    12061 aatgttggca gtttgaaaac taccggtggc cgtgtatcgg gcatcgaact cattgcgggc
    12121 attctggatg ccgggtccga aggggctgcc gctgcccacg cagctcggac tggggatctc
    12181 cgcgatcaga gtgttgcccg aggcatcgga gagaacaagg tggtagtccg aatcgctctc
    12241 gagcttgtac tgagtcagcg tggcgttgag aacccactgc gtggtctcgt acgggctgat
    12301 gcgattgttc gcggggatcg agctgggggt aggccagcct cgcatcgtcg tgatcgtttg
    12361 ggcggtggaa gatgagaggt ttatgaggcc gacatcggcg tcagtgccgg tcttcacgga
    12421 ccagcgctcc acgccgcact gtgcgagaat ctgtgtcgga acggaaacca gaacgagaat
    12481 gaagaacaac ggggccgcga atttgcgcat gaatagcgtc ctccagacga tgacagtcgg
    12541 tgagcggcgg tttgtgtggg ggctacagcg agtagagtcg agagcaacgg ctctgccaca
    12601 aacgccggcg cgaaaaagtg ggacgcagcc tcagtttgcc caaaaagcag ggcttggacg
    12661 cgggtcaagt tctaatactt tctcaacaat tgacccaata agctgagcag aaaatgaccc
    12721 gcaaactctt gttgaccctt cttctgacca gcgccgtttc cggctgcgtc ttcgtcccca
    12781 gcgtcatgtg gaaccgcgcc gaaacgaacc ggcagcacat caatgcagtg cacgtcggac
    12841 agtcgctcgc cgaggttcgg cagatcatga tgaaggaccc cgaaaagcgc gagatgagat
    12901 cccgattcga cggcaagacc gtggagttct ggagctacgt caccgactat ggccgcaggc
    12961 tcgactccac gatcaccttc atcgacggcc acgtgcagga gatccgaacc acgccgtgga
    13021 tggagaaaga ctgaatcgtc cgctccgcgc actcaaacgg gggtcgagcg ctgcattccg
    13081 gccacgtgtg gcacactgcg cggcacttta cgatgagtgc gaatgatgtg atgggcgtcg
    13141 gtggtcggcg atcagaggcc gaggatctcc ccaccgcgaa tgctggctct ccacctccgg
    13201 gattgccacg ctcggaacgg atcgcgttga tcgccatcgc ggccgtaacg ctggccatgc
    13261 gcatcgtcgc gtacttccac taccgcttcg attcggacga gccgcagcat ctccatgtgg
    13321 cgtgggggtg gacggcgggg ctgctgcagt atcgcgacgt cttcgacaac cacgcgccgc
    13381 tgtttcacat ggccaccgct ccgctgctcc ggctcctcgg cgagcgcgcg gacattcttt
    13441 tctggatgcg cgggccgatg gtgctgctct ggctggtcgt ggtcgcgtgc acctatgtgg
    13501 tggcgcggcg gctctacgac gtccgcatcg cgatctggtc aatcgtgttg ctgaatgttc
    13561 tgccgccgtt ttttctgaag tcgatcgagt tccgtaccga caatctgtgg aacgcgttct
    13621 ggatgctggc catcgtcgtc tttctcctcg tgcggcgttc cgcgggcacg ttcttcctcg
    13681 ccgggctgct gatgggctgc gcgttttccg tctcgctcaa gaccctgctg ctgctgctca
    13741 cgcttgcggc cgcgggcgcg atcacctggt tcatgcgccg ccgcgcacgc ggggcgggtg
    13801 tgcgagcggg gcttgtcgct gtcttcgtcc tcggcttctt gatcgtgccc gcgctcatcg
    13861 ccgcctactt catcgcgcgc ggtgcctggc cggatctcgt ctacggcgcc ctccggttca
    13921 acggcctggt cgcaggccac cgccctcccc tggtggtctg gatacggcgg ctttgctaca
    13981 tcccgatgat gtacgtcgtc tttcgtcaag cctggcggta ccgagggcgg acgacggatg
    14041 cgcgctattt ctttgccatt gcgacgggga tcttcttcgc gacgctcttc agcttctgga
    14101 ttctgatttc gccgcgcgat ttcctcccct tcatgccctt cctgtcgatc ttcttcgtcg
    14161 ccgcgttggc tcgccggaaa ttcttcctcg aggcgatcat cgtcaccacc atcgtgttgc
    14221 tgacgggcgt cggctactac tcggagtggc tgttgaaccg gacacgcgaa ttcatcacga
    14281 tggagaatca gctcctgaat gtctcgcgtc ccggcgagat ggtcatggac tacaagggcg
    14341 agctgattta ccggcgccgg ccgtactact tcatcctcga gttcatcacg cgccacgcga
    14401 tcgaacgcgg attgatcgcg gatacggtgc cggaggatct ggttcgcacg cgctgtcaca
    14461 tcgcacaggc ggacggaacg cagtggccca tccgcgcccg cgtcttcatg aacgccaact
    14521 tcatcgacat cggacgtctt cgcgtgtcgg gtcagtggct gcagacgaat ggagaattca
    14581 cgatcgccat tccgggtgac tatgtgatcg tgaacaagaa cggcgaagcg gtcggagcgc
    14641 tggatgggac gccgtatcgc ggc
//
LOCUS       DATLVW010000604         8896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_604239, whole genome shotgun sequence.
ACCESSION   DATLVW010000604 DATLVW010000000
VERSION     DATLVW010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604239"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VNN25_17905"
     CDS             complement(<1..1304)
                     /locus_tag="VNN25_17905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH93461.1"
                     /translation="MRKQCTSLFFAVLALVAATAAAQTHESITVQVVDVPVYVFSNGK
                     TIRDLTKNDFELFVNGKRQTIDYFDTIDLATVGSAPQAKKPVAPVVADPRARRLFLLL
                     FDLAYNRPAALARAQKAAAIMVDHAMRQDLFAVATYGSSGANFVIPFTRDHDVIRRAV
                     LKLAPSSAHDALALSITNAERETAAAWNPNMAGAAGGGEDPMADIMAGFKPEERQRAK
                     NAAKTELEDFSAVAARLTDLEGYKHVILFSEGFNSDLVTGVTNNVGGPPEVDSGMLEA
                     LQKLYRTFHAAGAHLHTVDLRIADPMNLDEGGRTPQATPVYAGGKPQSTQRFDPLSND
                     VLYQFAAQTGGQFIHYTNDFAGALEDLSTTVSTGYRLGFRPENPKKGENTIDVKVKNV
                     PSGTTVSFRKGFSTTPDVRNTGDALLLADIIQNDIPQTGTAP"
     gene            complement(1351..2199)
                     /locus_tag="VNN25_17910"
     CDS             complement(1351..2199)
                     /locus_tag="VNN25_17910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HXH93462.1"
                     /translation="MPRMPVRGEKLRQRLDTLVETFDVSTIAPDPLQLVLRYTNPPDQ
                     EVAGLLAAAFAYGRADIVVANAGRVLDRMLPSPYEYLVKFDRAEAKRRFAGFAHRFHK
                     TPELIDLLARIAAAIRAHGTLGELFRICYDDNDRDIGPSLTRFVEALIGSRRTKALDY
                     LLTSPANGSACKRMNLFLRWMVRRTEPDLGIWAFVDPAKLVMPVDTHVHRIATFLGLN
                     GRKTPDWKAARALTDRLAKFDSADPVRYDFAICRLGILDLCSRKRRKANCDVCLLRDV
                     CRFPVV"
     gene            2279..6325
                     /locus_tag="VNN25_17915"
     CDS             2279..6325
                     /locus_tag="VNN25_17915"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH93463.1"
                     /translation="MLAIRRLLCISFVLSSLAVPLHAAVTGTVINIDGKAVTGAKVSL
                     FAPELMAALGSRLQSADPQRTPLATVTTDSGGKFTLDVPKEQVVVDVRVEATGYAPAS
                     GRYAADDEAGAILLIQAAPVRGTITANGKPVPNATVAIYGTAEYVTKTDADGHYTAPD
                     VSKWALRFIVLHPDYAVVDELLGPNGTKKGPDFSMTAGVAVTGRVVAEDGQTPAGDAQ
                     LLIDGWPAGKSAADGTFTIAHAKKEWVHVEARLGYRVAQRARTAAGAPTLKLAKAGSV
                     SGSVRDVKSQLPVAGAHVAIAPSGAFGGREATLDVYTDAKGNFTMSPVVAGRYDVRPD
                     RPGYVITGAQPITVKGADAVQKALYAAARGRISGTVLDEDKRPVAAAHLATARASARN
                     DMFAMLGVMRGNATSDGYSAPDGHFVLRNVETEADLHVDALKRGYPGAKSAVLKLAAG
                     ERKTGVTITIPRGIAVTGRVTDKDHKALSGVAVEAIEASADAMGGVRRIVMSTIAGNR
                     NDELVRTGSDGTFSIKVKEGTYDLAFNREGFAAKTVRGTKVDATTKPVEVTLDPGVEI
                     TGRVTRSGAGVEGVNIRAMSQGGFQAAVTGPDGAFRIADLTPGQMMLIAAKQDSFIQE
                     MRPVTAPSKDIVIELPAGGRITGRVLDKASHEPITSFMAGVTTSRSGGGMVMMMPPMQ
                     KQFTSDDGTFVLEGIKPGSTQVVVNAPGYTTARVPNIEVEDGKTAPDVEVDVETGAKL
                     TGRVSGPDGSPIAGVSVREDQQGGGGGRVMRFDATDTGGTTDPNGDYTIDTLEPGEKT
                     FTFSRGGYIAQQKTVTVAAAKETRLDVQLSNGLRATGFVVSDGGSPIPDTMVRAVSAS
                     EMDGGRQATTDANGAFAIEALAPGHYTFTATKNGFAPATLRDVDIATSGPVRVILKSG
                     GVISGHVNGLTQQELDQTTVTASVSGGGGGGGPFGGGGGVNAPVDSGGNFRIDGAPSG
                     TIRISARTGAMFGGASKSAAPKTVELEPGGTAQVDIDFKSSTVISGRIVRNGAPVAGA
                     QVTFIPRGAKSQTVSSASSDSNGHYEVNSLDDGTYMVQVVDMARLSPFSTQYEVHGSD
                     TFDITIKTVTVRGRVVDATDTHPLNEATVDLRQATGQSIFGGGAGQTDADGRFTIENV
                     AAGTYQITADKSGYGHDSRQITISDSDPDQVQFQLSPSDGITIRAIDTRDNTPLTVNV
                     VRVVDAQGHELPSSSGFFGISSETVKLALAPGAYTVTVVARNYASQTLSMSSPSQQVV
                     RFSPGGTIILHSKDSSTRRARLIDAAGITHGLNPITQGISPLPPGTTQINNVSAGHYT
                     LQILDNTDRITKTVEIDVVDGQTSNYDV"
     gene            6341..6574
                     /locus_tag="VNN25_17920"
     CDS             6341..6574
                     /locus_tag="VNN25_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93464.1"
                     /translation="MNVLISDLDEKTVNELKKRAETGNRSLQAELKAIIQEATARDWQ
                     RTWAAADHIFEELRATGRKFSDSTELLREDRER"
     gene            6571..6996
                     /locus_tag="VNN25_17925"
     CDS             6571..6996
                     /locus_tag="VNN25_17925"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH93465.1"
                     /translation="MTLWVLDASVVIKWFIPEDDSQAALDLRVAGAGFATPDLLFAES
                     ANILWKHAKRGEVESHRATAIIDEIATAAWVVYSTRNLAGDALSLALASGISAYDASY
                     VALAIRLDTICVTADRKLFNKLQGSPLGKHVALLADYTN"
     gene            complement(7000..7611)
                     /locus_tag="VNN25_17930"
     CDS             complement(7000..7611)
                     /locus_tag="VNN25_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93466.1"
                     /translation="MPVTQPEPQTLAQVPFKVLDETLRQLRGTRNDIIRYAVWNTRNL
                     TDDEFNHADDRKLLGYFRQDLLETRFLSKGRRIDLIQLWHWFDDQMTGTEPMLIAGQE
                     VFVNVQDKDLEKVRKRISEIQSFLPTLRGDDLEAFKRVKYKLRRTVLRLTYDMHHLFK
                     RLEKYRKNFGGPAVPRLENVPHDVVADVPEPPLIDEHDEHDEQ"
     gene            complement(7652..>8896)
                     /locus_tag="VNN25_17935"
     CDS             complement(7652..>8896)
                     /locus_tag="VNN25_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93467.1"
                     /translation="NVTAIFTRAGEEGWGHFAAVKLTVAPQQVMALDDVVATLMETSG
                     VGQLEFLGDTDALIITSRTYTKVTTGGPGTYGQFIPAVATTDATTLADVPHILSTADF
                     RTNLGFAEVNGSDGTVRVTLFDAASGSVVSQHDYPIVPFTQMQFPATGAALMTAELQV
                     ISGDARIVAYGSVIDNHSGDPIYVAASRPQEGPFVAPVISQPGINTLWRSEVVISAFG
                     ESGGSFDLTYVDSVTAERLTKHGVLAPNQAVRIDDVVGSYFGRPNAFGTVRADLPSNL
                     VATSRTFTTSSNGTYGQFIPFTPAGEGFAPLAPGGAAREVLHVEHSPAFRTNAGAINT
                     GGSDALIRFSLFDAAGQLLGSTDRTVGPLQAVQFPIDVLTSSAFVDGRLEVQMLSGSR
                     GAVAWASVIDNATGDPIFVPVQ"
BASE COUNT         1702 a         2736 c         2884 g         1574 t
ORIGIN      
        1 atcggagccg ttcccgtctg cggaatgtcg ttctggatga tgtcggccag gagaagcgcg
       61 tcgcccgtgt tgcgaacgtc cggagtcgtc gagaatccct tgcggaacga aaccgtcgtg
      121 ccggacggga cgttcttcac cttgacgtcg atcgtgttct cgcccttttt cggattttcc
      181 ggccggaatc ccagacgata gccggtgctg accgtcgtgg aaagatcctc cagggcgccc
      241 gcgaagtcat ttgtatagtg aatgaactgg cctccagtct gcgccgcgaa ctgatagagc
      301 acgtcgttcg acagcggatc gaagcgctgc gtgctctgtg gtttgcctcc ggcgtaaaca
      361 ggagtggctt gcggcgtgcg gcccccttcg tcgagattca ttgggtcggc gatcctcaga
      421 tcgaccgtgt gcagatgggc gcctgcggcg tggaatgtgc gatagagctt ctgcagtgcc
      481 tccaacatcc cggaatcgac ctcgggaggg ccaccaacgt tgttcgtgac acccgtaacc
      541 aagtccgaat tgaaaccctc cgagaagaga atgacgtgct tgtagccctc gagatccgtt
      601 agccgggccg ctaccgcgga gaaatcttcg agctctgttt tggcggcgtt cttcgcgcgc
      661 tgccgctctt ccggtttgaa cccagccatg atgtcggcca tcggatcttc gccgccaccg
      721 gcggcgccgg ccatattcgg gttccacgcc gccgccgttt cgcgctccgc gttcgtgatc
      781 gagagcgcca gcgcgtcatg cgcactactg ggagccagtt tgagaacagc ccgccggatg
      841 acatcgtggt cgcgcgtgaa ggggatgacg aagttcgccc cgctcgaacc gtacgtcgca
      901 accgcgaaca ggtcctgccg cattgcgtga tcgaccatga tcgctgccgc cttttgcgcg
      961 cgggcaaggg cggccgggcg gttgtaggcg agatcgaaca ggaggagaaa cagtcgacgc
     1021 gcccgcggat ctgcgaccac cggcgccact ggctttttcg cttgcggcgc agagccgact
     1081 gtagcgagat cgatcgtgtc gaaatagtcg atcgtttgcc gctttccgtt gacgaagagc
     1141 tcgaaatcgt tcttcgtcag atcgcgaatc gttttgccgt tgctgaagac atacaccggc
     1201 acgtcgacga cctggacggt gatcgactcg tgcgtttggg ctgcggcggt tgctgcgacg
     1261 agcgcaagga ctgcaaagaa cagggacgtg cactgctttc tcattcttga tctcccgggc
     1321 ccagtgcggc cacccgcagt ctatccgcca tcacacaacc ggaaagcggc agacatcgcg
     1381 caggaggcaa acgtcacagt ttgctttgcg gcgtttgcgg ctgcagagat cgaggatgcc
     1441 gaggcggcag atggcgaagt cgtagcggac gggatcggcg gaatcgaact tcgccagacg
     1501 atcggtgagc gcacgggctg ctttccagtc cggcgtcttg cggccgttca gcccgaggaa
     1561 cgtggcgatg cggtggacgt gcgtgtcgac cggcatcacg agcttggcgg gatcgacgaa
     1621 ggcccagatg ccgaggtcgg gctcagtgcg gcgcaccatc cagcggagaa aaaggttcat
     1681 ccgcttgcag gcggagccgt ttgcgggtga tgtgaggagg tagtcgagag ccttcgttct
     1741 ccgagagccg atcagcgctt cgacgaatcg ggtcagcgac gggccgatat cgcggtcgtt
     1801 atcgtcgtag cagatccgaa aaagctcgcc gagcgtgccg tgcgcccgaa tggcggcggc
     1861 gatgcgcgcg agcagatcga tgagctccgg cgttttgtga aaccggtggg cgaatcctgc
     1921 aaaacgccgc ttcgcttcgg cgcggtcgaa cttcacgaga tattcataag gagaaggcag
     1981 catgcggtcg agcacgcgac cggcgttggc aacgacgatg tccgcgcggc cataggcaaa
     2041 tgcggcagcg agaagaccgg ccacttcctg atcgggagga ttggtgtagc ggaggacgag
     2101 ttgcagcgga tcgggagcga tcgtcgagac gtcgaacgtc tcgacgagcg tgtccagccg
     2161 ctggcgcagc ttttctccgc gaaccggcat tctcggcatg tggggccaaa cgtcgaaacc
     2221 gtgttcagca ttcttcgttt acagttcgta cattcgttcg aaacctcggg aggaaaccat
     2281 gctcgcaatc cggcgcctgc tctgcatctc gttcgtactg tcgtcgctgg ccgtgccgct
     2341 tcacgcggcc gtgaccggta ccgtcatcaa catcgacggc aaggcggtca cgggagcgaa
     2401 ggtttctctc ttcgcgccgg agctcatggc ggcgctgggc tcgcggctgc aatcggcgga
     2461 tccgcagcga acgccgctgg ccacggtgac gacggactcc ggcggcaagt tcacccttga
     2521 cgtcccgaag gaacaggtcg tcgtcgatgt ccgcgtcgag gccaccggct acgcaccagc
     2581 aagcggccgg tatgccgccg acgatgaggc cggcgcgatt ctcctgatcc aggccgcgcc
     2641 cgttcgcgga acgatcacgg caaacggcaa accggtcccg aacgccaccg tcgccatcta
     2701 cggcaccgcc gaatacgtga cgaaaacgga cgccgatggt cattacacgg ctcccgatgt
     2761 ttcgaagtgg gcgttacgct tcatcgtgct gcaccccgat tacgcggtcg tggatgagct
     2821 cctcggtccg aacgggacga agaaaggccc cgacttctcg atgacggccg gcgtcgcagt
     2881 taccggccgc gtcgtcgccg aagacggcca gacgccggcc ggagacgcgc aactcttgat
     2941 cgatggctgg ccggcaggta agtcggccgc cgacggaacc ttcacgatcg cccacgcgaa
     3001 aaaagagtgg gtgcacgtcg aagcgcggct cggttatcga gttgctcagc gcgcccgaac
     3061 ggctgctggt gccccaacgc tcaaactcgc caaggctggc agcgtgagcg gcagcgtgcg
     3121 cgatgtgaaa tcgcagcttc ccgtcgccgg tgcgcacgtg gccattgcgc cgtcgggcgc
     3181 gttcggaggg cgggaagcga cgcttgatgt gtacaccgac gcgaagggca acttcacgat
     3241 gtcgccggtc gtcgcgggca ggtacgacgt gcggccggac cgccccggtt acgtcatcac
     3301 cggcgcccag ccgataacag tcaaaggcgc cgacgctgtg cagaaagcgc tctacgcggc
     3361 ggcccgcggc cggatcagcg gcaccgtcct cgatgaagac aagcgccccg ttgcggcagc
     3421 gcacctggcg acggcgcgtg catcggcacg caacgacatg tttgccatgc tcggggtcat
     3481 gcgcggcaac gccaccagcg atggctacag cgcgcccgac ggccacttcg tccttcgcaa
     3541 tgtcgagacg gaggcggacc tccacgtcga cgcgttgaag cgcggctatc ccggagccaa
     3601 atcggcggtg ctgaagctcg cggcgggcga gcgcaagacc ggcgtcacga tcacgatccc
     3661 gcgcggcatc gccgtcaccg gccgcgtgac cgacaaggac cacaaggcgc tgtccggcgt
     3721 cgccgtcgag gccatcgaag cgtccgccga tgcgatgggc ggcgtgcgcc gcatcgtcat
     3781 gtcgaccatc gcgggcaatc gaaacgacga gctcgtacgc acgggaagcg acgggacgtt
     3841 ctcgatcaaa gtgaaagagg gaacgtacga cctcgccttc aatcgcgagg ggttcgccgc
     3901 caagacggtg cgcggaacga aagtggatgc aacgacgaag ccggtcgaag tcacgctcga
     3961 tcctggcgtc gagatcacgg gccgcgtcac gcgcagcggc gcgggcgtgg aaggcgtcaa
     4021 catccgggcc atgtcgcagg gcggattcca ggccgccgtc acaggaccgg atggcgcgtt
     4081 ccgcatcgcc gacctcactc ccggccagat gatgctgatc gctgccaaac aggattcgtt
     4141 catccaggaa atgcgcccgg tgacggcgcc gtcgaaagac atcgtcatcg agctgcctgc
     4201 cggtggccgc atcacgggac gcgtgctcga caaagccagc catgagccga tcacgtcgtt
     4261 catggccggc gtgacgacat cgcgcagcgg cggcgggatg gtgatgatga tgccgccgat
     4321 gcagaagcag ttcaccagcg acgacggcac gttcgttctc gaagggatca agcccggctc
     4381 gacgcaggtg gtcgtcaacg ctcccggcta cacgaccgcg cgcgtcccga acatcgaagt
     4441 ggaggacggg aagacggcgc ccgacgtcga agtcgacgtc gagacgggag cgaagctgac
     4501 gggacgcgtc agcggtccgg acggcagccc gatcgcgggc gtctcggttc gcgaagatca
     4561 gcaaggcggc ggaggcggcc gcgtcatgcg cttcgacgcc accgacacgg gcggaaccac
     4621 ggatccgaac ggcgactaca cgatcgatac gctggagccc ggcgagaaga cgttcacctt
     4681 ttcccgcgga ggctacattg cccagcagaa gacggtgacg gtggcggcgg cgaaagagac
     4741 gcggctcgat gtgcaactct cgaacggcct ccgcgccaca ggcttcgtgg tctcggacgg
     4801 cggctctccg attcctgaca cgatggtgcg cgcggtctcc gcgagcgaaa tggacggcgg
     4861 gcgccaggcg accacggacg ccaacggcgc ctttgcgatc gaagcgctcg cgcccggcca
     4921 ctacacgttc accgcgacga agaacggctt cgcgccggcc acgcttcgcg atgtcgacat
     4981 cgccacatcg ggcccggtgc gcgtgatcct gaagagcggc ggcgtgatca gcggccatgt
     5041 gaatgggctg acgcagcagg agttggatca aacgacggtc acggcgagcg tttccggggg
     5101 cggcgggggc ggcggtccgt ttggcggcgg cggtggcgtg aatgctcccg tcgacagcgg
     5161 cggcaacttc cgcatcgatg gcgctccgtc gggcaccatc cggatctccg ctcgcacagg
     5221 agcgatgttc ggcggagcga gcaagtccgc ggcaccgaaa accgtagagc tcgagccggg
     5281 tggcaccgcg caggtcgaca tcgatttcaa gtcgtccacg gtgatcagcg gccgcatcgt
     5341 gagaaatggt gcccccgtcg ccggcgctca ggtcaccttc attcctcggg gcgcaaaaag
     5401 ccagaccgtc tccagcgcaa gctcggactc caacggccac tacgaagtga acagcctcga
     5461 tgacggcacg tacatggtgc aggtcgtgga catggcgcgt ctgagtcctt tctcaacgca
     5521 atacgaagtc cacggctccg atacgttcga catcaccatc aagaccgtga ccgtgcgcgg
     5581 ccgcgtagtg gatgccaccg acacgcatcc gctcaacgaa gccacggtcg atctgcgcca
     5641 ggccaccggc cagtcgatct tcggcggagg cgccggccag acggatgccg acggacgctt
     5701 tacgatcgag aatgtcgccg ccggtacgta ccagatcact gccgacaaga gcggctacgg
     5761 tcacgactcg cgccagatca cgatcagcga ctccgatcct gaccaggttc agttccagct
     5821 ctcaccgagc gacggcatca cgattcgtgc aatcgataca cgggacaaca caccgctcac
     5881 cgtgaatgtg gtgcgtgtcg tcgatgcgca ggggcacgag cttccgtcct cgtcgggatt
     5941 cttcggcatt agcagcgaga ccgtgaaact ggcgctcgct cccggcgctt acacggtgac
     6001 ggtcgtcgcg cgcaactacg cgtcgcagac gctgtcgatg tcctcccctt cgcaacaggt
     6061 ggtccgcttc tccccgggcg gaacgatcat cctgcactcg aaggactcat ccacccgacg
     6121 cgcccgcctc atcgacgcgg caggcatcac gcacggcctc aatccaatca cacagggaat
     6181 ctccccacta cctcccggga caacgcaaat caacaacgtg tccgcgggcc actacacgct
     6241 ccagattctc gataacacgg accggattac gaagacggtg gagatcgatg tggtggatgg
     6301 gcagacgagt aactacgacg tgtgatatca ttttgatatc atgaacgttc tgattagcga
     6361 tctcgacgag aaaacagtca acgaactcaa aaaacgggct gagacgggca atcgatctct
     6421 ccaagcggaa ctgaaagcga tcatccagga ggcaacagct cgcgactggc aacgcacttg
     6481 ggccgcggcc gatcatatct tcgaagagtt gcgcgctact ggccggaagt tctctgacag
     6541 taccgagctt ctgcgtgagg atcgcgagcg ttgacgctgt gggttctcga tgcaagcgtc
     6601 gtcatcaagt ggttcattcc agaagatgac agtcaagctg cgctcgacct gcgagtggcc
     6661 ggtgctggat tcgcgacacc cgatctgctt ttcgctgagt ctgccaacat tctctggaag
     6721 catgcgaagc gaggagaggt cgagtcacac cgagcgacgg cgatcatcga tgagatcgcc
     6781 accgcagcgt gggtcgttta ttcgacccgg aaccttgcag gcgatgcact ctctctcgca
     6841 ctggccagtg ggataagtgc atacgacgcg tcgtacgttg ccctcgcgat tcgtctcgat
     6901 acgatttgcg tcaccgccga ccgaaagttg ttcaacaagc tccaaggctc gcctttgggc
     6961 aagcacgtcg ctcttctcgc ggactacacg aactaaccct cactgctcgt cgtgttcgtc
     7021 gtgctcgtcg atgagcggcg gctcggggac atcggccact acgtcgtgcg ggacgttctc
     7081 gagccgcggc actgcggggc cgccgaagtt cttgcggtat ttctcgaggc gcttgaagag
     7141 gtggtgcatg tcgtaggtca ggcgcaggac cgtgcggcgg agcttgtact tcacgcgctt
     7201 gaacgcttcc agatcgtcgc cgcgcagcgt gggcaggaaa gactggatct ccgagatccg
     7261 cttgcgcacc ttctcaaggt ctttgtcctg aacgttgacg aagacttcct ggccggcgat
     7321 gagcatcggc tcggttccgg tcatttgatc gtcgaaccaa tgccagagct ggatcaggtc
     7381 gattcggcgg cctttggaga gaaagcgggt ttccagcaga tcctgtcgga aatagccgag
     7441 gagcttgcgg tcgtcggcgt ggttgaactc gtcgtcggtc aggttgcggg tgttccagac
     7501 cgcgtagcga atgatgtcgt tacgcgttcc gcgaagctgg cgcagcgtct catcgaggac
     7561 tttgaaaggt acctgggcga gcgtttgcgg ctcgggttgg gtcacgggca tgggcctgaa
     7621 gaatacaaca ctgaatcgaa gagcggctcc gtcactgtac cggaacgaag atcggatcgc
     7681 ctgtggcgtt gtcgatgacg gaggcccagg cgactgcgcc tctgctcccg gagagcattt
     7741 gaacttccag gcggccatcc acaaacgccg aggatgtcag gacgtcgatc ggaaactgaa
     7801 cggcctgcag cggaccgaca gtacggtccg tacttccgag gagctggcct gctgcgtcga
     7861 acaacgaaaa acggatcagg gcgtcgctgc cgccggtatt gatcgcgccg gcgtttgttc
     7921 ggaaggccgg tgagtgctcg acgtgaagca cttcccgtgc agcgcctccg ggagccagcg
     7981 gtgcaaagcc ttcgccggcg ggtgtgaacg ggatgaactg accgtacgtt ccgttgctcg
     8041 aggtggtaaa tgttcgcgac gtcgccacga ggttgcttgg aaggtcggcg cgcacggtgc
     8101 cgaatgcgtt cgggcggccg aagtagctgc cgacgacatc atcgatgcga actgcctgat
     8161 tcggagcgag gacgccgtgc ttcgtgagcc gttctgcggt gacggaatcg acgtaggtta
     8221 gatcgaaact gccgccgctc tcaccgaacg cgctgatgac aacttcgctg cgccagagcg
     8281 tgttgatgcc cggttgactg atcactggcg cgacgaacgg gccttcctgc ggccgcgacg
     8341 cggcaacgta gatcggatca ccgctgtggt tgtcgatcac agagccgtaa gcaacaatcc
     8401 gcgcgtcgcc gctgatcacc tgcaactccg ccgtcatcag cgcggctcct gttgcaggaa
     8461 actgcatctg cgtgaaaggc acgatcgggt aatcgtgctg cgagacgacg gacccgctcg
     8521 ccgcatcgaa caaggtgact cgaacagttc cgtcgctgcc gttcacctct gcgaagccga
     8581 ggttcgtacg gaagtctgcg gtgctgagga tgtgcggaac gtcggcgagc gttgttgcat
     8641 ccgtcgtcgc caccgcgggg ataaactggc cgtaggttcc cgggccgccc gtggtgacct
     8701 tcgtgtatgt gcggctggtg atgatcagcg cgtctgtgtc gccgagaaac tcgagctggc
     8761 cgacgccgct cgtctccatc aaggtggcaa cgacatcatc gagcgccata acctgctgcg
     8821 gcgccaccgt gagcttcacg gccgcgaaat gtccccaccc ttcctcgcca gctctcgtga
     8881 aaatcgccgt gacgtt
//
LOCUS       DATLVW010000605         3000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_436730, whole genome shotgun sequence.
ACCESSION   DATLVW010000605 DATLVW010000000
VERSION     DATLVW010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436730"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1664)
                     /locus_tag="VNN25_17940"
     CDS             complement(333..1664)
                     /locus_tag="VNN25_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXH93468.1"
                     /translation="MTDPTPTLARRLGAFDATMIVMGGIIGSGIFLNPTVVAKQVHTP
                     ALILGAWIAGGVIALLGAFAYAELAALRPEVGGQYAYLRDAYHPSVAFMYGWALLLIV
                     QSGGMAAVAATFARYALSLIGIAIPEWTVAVGALLILTLINCLGVRSGSNTQNALMLM
                     KLAAIAMLLVAGATVVAPHAAPRAMLDAPLSTDLFLRFGAAMTPVMFAYGGWQTASFV
                     SGELARPRRDLPIGLLVGIIGVIAIYVGVSFVCVRALGADGLANAATPASAVMRLALG
                     ERGARLIAAGIAISTLGFLSQSMLTAPRVYYAMAADGLFFKSVGRISAKTQVPVVAIA
                     LQGIVAIIITVSGTYEQILSYVVSTDFIFFGLTGLALFIFRRRQPAAGFRTPGHPFTT
                     GIFTLSCWIVVIATIVKSPVNSLIGFAILAAGLPAYAWWRRAKSPPPSISS"
     gene            complement(1854..2774)
                     /locus_tag="VNN25_17945"
     CDS             complement(1854..2774)
                     /locus_tag="VNN25_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93469.1"
                     /translation="MSEQARVLIVEDSETDRALLDHHLSRDGFDTEFACDGVEAWQML
                     DANPDRYDLVLLDRTMPRMNGMDLLKRIKSDHRFLIVPVILQSALVARDEVIEGIRAG
                     AYYYLTKPYDVEMLMSVVRTAAADSAAARELQRRVRRGLQVLQLLDDAHFTYRTIEEA
                     RGLAAVLANACPDAEATVIGLTELLVNAVEHGNLGITYDDKSALLSEGTWAAEVERRL
                     ALPENVAKRVDVGFHRNDTELRFTIADRGNGFEWTRYLDIDPRRAFDRHGRGILMARH
                     VSFSSIEYRGCGNEVVATVDCCAPEAAATA"
     gene            complement(2816..>3000)
                     /locus_tag="VNN25_17950"
     CDS             complement(2816..>3000)
                     /locus_tag="VNN25_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93470.1"
                     /translation="DLAPMLRAGLSAAPADAAPEVYAAALWKLTSLGGQGAFRELVER
                     LLRERGEWEAVVQGYR"
BASE COUNT          562 a          892 c         1012 g          534 t
ORIGIN      
        1 cgtagtttga gatccttcga ccgtccttcg gcacttcgtg tccttcggcg gctcaggatg
       61 acacgattgg ttgggtcagg atgaatttgt tgtgtcatcc tgagtcgccg gagccgccga
      121 ctttacgctg cactcacccc cggcaggcat cggcggcgaa ggacgatcga aggatctcaa
      181 acttcgatga tcgtgttcat ccggcgcgag cgattcgcca tcaatgacgt tcgctcgcgc
      241 tgaaccaacg agatcttcgt aatttgagat ccttcgaccg tccttcggca cttcgtgtcc
      301 ttcggcggct caggatgaca cggttggttc gtttaggatg agattgatgg tggagggctc
      361 ttcgctcttc tccaccacgc gtacgccggc aatcccgcgg cgaggatcgc gaatccgatc
      421 aggctgttga cgggcgattt cacgatcgtg gcgatgacga cgatccagca ggagagtgtg
      481 aagatgccgg tggtgaaagg atggcccggc gtgcggaagc cggcggcggg ttggcggcgg
      541 cggaagatga agagcgccag tccggtcagg ccgaagaaga tgaagtcggt cgagacgacg
      601 tagctgagga tctgttcata ggttccggag acggtgatga tgatggccac gatcccctgc
      661 agcgcaatcg cgacgaccgg cacctgcgtc ttcgcgctga tgcgtccgac gctcttgaag
      721 aagagtccat ccgcggccat cgcatagtag acgcgcggtg ccgtgagcat gctctggctg
      781 agaaatccga gcgtggagat ggcgatgccg gcggcgatca ggcgggcgcc gcgctcgccg
      841 agggcgaggc gcatgacggc ggaggcgggg gtggcggcgt tcgccaatcc atcggcgccg
      901 agcgcgcgca cgcagacgaa actgacgccg acgtagatgg cgatgacgcc gatgatgccg
      961 acgagcaggc cgatgggaag gtcgcggcgc gggcgcgcaa gctcgccgga cacgaagctc
     1021 gcggtctgcc agccgccgta cgcgaacatc acgggagtca tcgccgcgcc gaaccggagg
     1081 aagagatcgg tggaaagggg agcgtcgagc atcgcccgtg gcgcggcgtg cggcgcgacg
     1141 acggtcgctc cggccacgag gagcatggcg atcgcggcga gcttcatcag catgagcgcg
     1201 ttttgcgtgt tgctgccgct gcgtacgccg agacagttga tgagcgtgag gatgagcagc
     1261 gcgccgacgg cgaccgtcca ttccggaatg gcgatgccga tcagactgag cgcgtagcgc
     1321 gcgaacgtcg cggccaccgc cgccatcccg ccgctctgca cgatgagcag cagcgcccag
     1381 ccgtacatga aggcgacgga cggatggtag gcatcgcgga ggtacgcata ctgaccgccg
     1441 acctcgggac gcaaggcagc cagctcggca taagcaaacg cgccgaggag cgcgatgacg
     1501 ccgccggcga tccacgcgcc gaggatcagc gcgggcgtgt gaacctgctt tgcgacgacg
     1561 gtgggattga gaaagatgcc ggagccgatg atcccgccca tcacgatcat cgtggcgtcg
     1621 aacgcgccga ggcggcgggc tagggtgggg gttgggtcgg tcaatgtcag ccttgattca
     1681 tgatcagaaa accgttgaac cacagagaca cagagcacac agagattaga gacaagagat
     1741 tagagatcag gaaggctgag ggttgtcctg atcgctgatc tcttctctct gatctcctgt
     1801 ctctaatctc tgtgtcctct gtgcctctgt ggtgattcct tcgtctgagc aaatcacgcc
     1861 gtagccgccg cctcgggcgc acaacaatcc accgtcgcca ccacctcgtt gccacaaccc
     1921 cggtactcga tcgacgagaa gctgacgtgg cgtgccatga ggatgccccg gccatgccgg
     1981 tcgaatgcgc ggcgaggatc gatgtcgagg taacgcgtcc attcgaaacc gttgccgcgg
     2041 tcggcgatcg tgaaacgcaa ctcggtgtcg ttgcgatgga atccgacgtc gacgcgcttg
     2101 gcgacatttt ccggcagcgc cagccttcgc tcgacctcgg ctgcccaggt cccctcggac
     2161 agcagcgcgc tcttgtcgtc gtaggtgatg ccgaggttgc cgtgttcgac ggcgttgacg
     2221 aggagctccg tgagcccgat gaccgtcgcc tcggcgtcgg ggcacgcgtt ggcaagaacc
     2281 gcggccagac cgcgcgcttc ttcgatcgtc cgataggtaa agtgcgcatc gtcgagcaat
     2341 tgcagaacct gcaatccccg gcgcacacgc cgctgcaact cgcgcgcggc ggcactgtcg
     2401 gcggcggcgg tgcgcacgac gctcatcagc atctcgacgt cgtacggttt ggtcaggtag
     2461 tagtaggcgc cggcgcggat gccttcgatc acttcgtcgc gcgcgaccaa tgcgctttgc
     2521 aggatgaccg ggacgatgag gaagcggtga tcgctcttga tgcgcttgag caggtccatc
     2581 ccgttcatgc gcggcatggt gcggtcgagg aggacgaggt cgtagcggtc ggggttggcg
     2641 tcgagcatct gccacgcttc gacgccatcg catgcgaact cggtatcgaa cccgtcgcgg
     2701 ctcaagtggt gatcgagcag agcacggtcg gtttcgctat cttcgacgat caggacgcgc
     2761 gcctgctcag acatcgtgac ctcaattgtt gtggtgcgtg cggatcataa agcaactagc
     2821 ggtaaccctg aacaactgcc tcccactcgc cgcgctcgcg caggagccgc tcgactagtt
     2881 cgcggaatgc tccttgtcca ccgagggatg tcaatttcca gagagccgca gcgtaaacct
     2941 caggcgccgc atctgccgga gcggcggaga gtccggcgcg cagcatcggc gccagatcgg
//
LOCUS       DATLVW010000606         5706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_195054, whole genome shotgun sequence.
ACCESSION   DATLVW010000606 DATLVW010000000
VERSION     DATLVW010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195054"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1329)
                     /locus_tag="VNN25_17955"
     CDS             complement(88..1329)
                     /locus_tag="VNN25_17955"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXH93471.1"
                     /translation="MPSSPSPSIAGTVVHTLSQALWDRVAATFRSATRRERKLRIGVD
                     IRPFYEPLTGIGWYLYHLLHEIGKRDDVELYLFGDARVTDYGPVLHADLPPSAHLCWF
                     DLRGKGIIPGRWRALTAAAYVLWMKLIDADVMFAANYFLPRLLGAVARRRVVTIHDLT
                     YKRFPELLQKETLANLGYQMTRELAHADAAICVSESTRQDLLHFYDIDPSRAITIHSG
                     LTVPAATTPHSPLPTLPPRYILFVSTIEPRKNLDVLLDAYARLRARGAYDGALVVVGR
                     VGWKAEATRERLRAEGVHHLDYLPAGQLAAVYRGAELFVFPSIYEGFGFPLLEAMAYG
                     VPSIAARSSSLPEIGGDAALYFEPHDSKGLEAQISRVLTDAVLREQLAAAGPIRAAQF
                     RWNVAAEQTLAVLRKASGDSA"
     gene            1426..2649
                     /locus_tag="VNN25_17960"
     CDS             1426..2649
                     /locus_tag="VNN25_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93472.1"
                     /translation="MKKLCVIGGVLLAFVGGVIIYLIATTPKTSAGVRFPLTAAQRAL
                     LASVPESAESFALIPTAAALDGKLRANPVTAQAVEGWERSHSIPSPWMVGGADLLAWR
                     DATGTRYLIRLDPIRSAVVRTYMMLGGDLGDTLLLNAPPAQPISPAALDEVLRLAEKL
                     PPGDAFVVQRKGARGAFPPIARPAVTSLSVTSDEILLTSRAAREDEEPAMQATQPAQF
                     VRGALLSATFATMPRIVEDLNRVFGTRVSSLLENGGSMTIYDVDTHKFLPRPLGVIAV
                     PDDPVRRAAFEDFVARVRQGESLGIHIQTAEQNGQLLLSFDDSINQYKNDAIDPGRWP
                     AGKWAVRADSKRLVPILSKLGDSIGLRIAAGRVYRSARDLKRWIEALQEATSVEAADS
                     DDGNVEELKVRIGAK"
     gene            2685..3245
                     /locus_tag="VNN25_17965"
     CDS             2685..3245
                     /locus_tag="VNN25_17965"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXH93473.1"
                     /translation="MPDKDFIVFDDIDKYGSETYERTYQFAVSELDRIDLAAIGPVSL
                     VATASKGDLVGEYLADGSTTFTGDLTCSRCVEPFPFANSSEFHLRFRPRPAVLAENEV
                     EITPDELDIEFYTERLIPLRDLAAEQVQLSIPMKPLCTESCLGLCPQCGANRNQESCS
                     CQSAAGDERWGALAGIRAELAKKNEQ"
     gene            3534..3719
                     /gene="rpmF"
                     /locus_tag="VNN25_17970"
     CDS             3534..3719
                     /gene="rpmF"
                     /locus_tag="VNN25_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXH93474.1"
                     /translation="MPNPKRRHSKARTRTRRAHDFLEAKSLSTCPQCHEAKMPHRVCP
                     KCGFYKGREVIAVKDSF"
     gene            3732..4769
                     /gene="plsX"
                     /locus_tag="VNN25_17975"
     CDS             3732..4769
                     /gene="plsX"
                     /locus_tag="VNN25_17975"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXH93475.1"
                     /translation="MSDVCIALDAMGGDHAPAEVVKGAVLAAAEFAVEIILVGQEDVV
                     RRELAAAGGTPRNISVFDAREIVTMEDTALAPLRRKRNSSVRVCANLVAEGKANAFVS
                     AGNTGATWTSARAVMGMIEGVSRPALAAVVPSLKGHTLLLDVGANVDAKPNHLREFAV
                     MGHFYAQMLFDVVAPRVGLLSIGEEEGKGNELTKETYRVLKETGLNFIGNAEGRDVFN
                     GNADVVVCDGFIGNVVLKASEALGEFVSTTLRQEMMKTLPRKIGGMLAKSAFEGLKKR
                     MDYSEYGGAPLLGVKGGCIVCHGRSNAKAIKNAIRVARNFAINRIDEKIMSKVNDLHK
                     REHDSSVLATQ"
     gene            4775..5227
                     /locus_tag="VNN25_17980"
     CDS             4775..5227
                     /locus_tag="VNN25_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93476.1"
                     /translation="MQSRPARLLLFAITITGCATHSANLPPEPNVVELHTVPAHASVS
                     LDCGAGTVAHGTTPMRVDVPRYPERCTIDVTANGYRPLNMRFDRGLVLKEGVPLRVEA
                     SHTLDRTATTLDMILFPLRNLADRIENAASSSGSADYRLEIKLMPVGQ"
BASE COUNT         1095 a         1754 c         1846 g         1011 t
ORIGIN      
        1 ctgcggatgc gcagcgatgt tcaaaagtcg caggcgagcc gcctgcggtc cagccgtcga
       61 gccgacggcg ctacgtcagc ggacgcgcta cgctgaatca ccggacgcct tccgcaagac
      121 cgccagcgtc tgctcggcag ccacattcca acgaaactgc gccgctcgaa tcggtcctgc
      181 agcagcgagt tgctcgcgaa gcacggcatc tgtaagcacg cgcgaaatct gcgcttcgag
      241 gcctttggaa tcgtgcggtt cgaagtagag cgcggcgtcg ccgccgatct ccggcagcga
      301 cgacgaacgt gcggcgatcg acgggacgcc gtacgccatc gcttcgagca gcggaaatcc
      361 gaaaccttcg tagatcgatg gaaagacgaa cagctcggcg cctcggtaaa ccgcggcgag
      421 ctgtccggcc gggagatagt cgagatgatg cactccctcg gcgcgaaggc gttcgcgcgt
      481 tgcctccgcc ttccacccca cgcgccccac tacgaccagc gcaccgtcgt acgcaccgcg
      541 cgcgcggagt cgggcgtagg cgtcgagcag aacatcgagg ttcttccgcg gctcgatcgt
      601 gctgacgaaa agaatgtagc ggggtgggag ggtagggagt ggggagtggg gagtggtagc
      661 ggctggaact gtcagaccgg agtgaatcgt gatcgcccgc gacggatcga tgtcgtagaa
      721 atgcagcaga tcctgccgcg tcgattccga aacgcagatg gccgcgtcgg cgtgggccag
      781 ctcgcgcgtc atctggtagc cgaggttggc gagcgtctct ttctgcagca gttcggggaa
      841 gcgtttgtag gtgaggtcat ggatcgtgac gacgcggcgg cgggcgacag cgccgaggag
      901 gcgggggagg aagtaattgg cggcgaacat cacgtcggca tcgatcagct tcatccacag
      961 cacgtacgct gctgcggtga gcgcacgcca gcgaccggga atgatccctt tgccgcgaag
     1021 gtcgaaccag cagaggtggg cagacggcgg cagatcggcg tgcaggaccg gcccgtagtc
     1081 cgtcacgcgc gcgtcgccga acagatagag ctccacgtcg tcgcgcttcc cgatctcgtg
     1141 gagcaggtga tagagatacc agccgatgcc ggtgagaggc tcatagaaag ggcggatgtc
     1201 gacgccgatt ctgagctttc gttcgcggcg ggttgccgac cgaaacgtgg ccgcgacgcg
     1261 atcccacagc gcctggctca gagtgtgaac gacggtgccc gcgatggacg gagagggtga
     1321 tgacggcatc ggaggcggat cgtagcgcat gcgcctgcgg cgcgttgtcg gttgtcggtt
     1381 gggtgcggct tcgccgcgtc tgacacaatg tgggctgcac gaaccatgaa aaagctttgc
     1441 gtcatcggtg gagtcctcct cgcgttcgtt gggggcgtca tcatctattt gatcgcgacg
     1501 acgccgaaga cttcggcggg cgttcgattt ccgctgaccg ccgcgcagcg cgcgctgctc
     1561 gcgtccgtcc cggagtcggc ggagtccttt gcactgattc cgacagcggc cgcgctcgac
     1621 ggaaagctgc gcgcgaatcc ggtcacggcg caggcggtcg aggggtggga gcgctcgcat
     1681 tcgattccgt caccgtggat ggttggcggc gcagatctcc tggcgtggcg cgatgccacc
     1741 ggaacgcgct atctgatccg gctcgatccg attcgatccg cggtcgtccg tacctacatg
     1801 atgctcggcg gcgacctcgg cgacacgctt ctcctcaacg cgccgcccgc gcaaccgatc
     1861 agtcctgccg cgctggacga ggttctcagg ctcgcggaaa agcttccgcc gggggacgca
     1921 ttcgtcgtcc agcgcaaggg cgcgcgcggc gcatttccgc cgatcgcgag gccggctgtg
     1981 acttctcttt ccgtcacgtc tgatgagatt cttctgacgt ctcgagcggc gcgggaagat
     2041 gaggagcctg cgatgcaggc gacccaacct gcccaattcg tgcgcggcgc gttgctttcg
     2101 gccaccttcg ccacgatgcc gcgcatcgtc gaagacctga accgcgtctt cgggacacgt
     2161 gtctcatcgt tactggagaa cggcggatcg atgacgatct atgacgtcga cacccacaag
     2221 ttcctcccgc ggccgctcgg cgtcatcgcc gttcccgacg atccggtgcg ccgcgcggcg
     2281 ttcgaggatt tcgtcgcccg cgtgcgccag ggcgagtccc tcggcatcca catccagacc
     2341 gccgaacaga acgggcagct cctcctctcc ttcgacgact ccatcaacca atataagaat
     2401 gatgcgatcg accctggccg ctggccggcg gggaaatggg ccgtgcgcgc cgactcgaag
     2461 cggttggtgc cgattctgag caagctgggc gatagcatcg ggctgcggat tgccgccgga
     2521 cgcgtttacc gctccgcgcg cgatctgaag cgctggattg aggcgttgca ggaggccacc
     2581 agcgtcgaag cggccgactc cgacgatggg aatgtggagg agctaaaggt aagaatcggt
     2641 gcgaaataga agccgggagg gcgcagttac agcgtcgaac gtccatgccc gacaaagact
     2701 tcatcgtctt cgacgatatc gataaatacg gctccgagac ctacgaacgg acctaccagt
     2761 tcgcggtcag cgagctggac cgcatcgatc tggccgccat cggcccggtg tcgctcgtgg
     2821 ccacggccag caagggcgac cttgtgggcg aatacctggc ggacggttcg acgacattta
     2881 ccggcgatct gacctgttcc cgctgtgtcg agcccttccc atttgcgaat tcgtccgagt
     2941 ttcacttaag attccgcccc cgacccgccg ttctggcaga gaacgaggtc gagatcacac
     3001 cggacgagct cgacatcgag ttctacacgg agcggctgat tccactgcgg gatctcgccg
     3061 ccgagcaggt ccagctttcc atccccatga agccgctgtg cacggagagc tgtctcgggc
     3121 tctgtccgca gtgcggggcg aaccggaatc aggaaagctg ctcgtgtcag tcagcggcgg
     3181 gcgacgagcg ttggggcgcg ctggccggga tacgggcgga gttggcgaag aagaacgagc
     3241 agtaaggcag tgaggcagag atcagagacg agagatcaga gatcaggcga agtgcctttc
     3301 ctgatctcta gtctctgatc tcaaaagtag tcaatagttg cgatcaaacg tcagagatga
     3361 gagatcagag accaggtgaa gttcctcctg atctctgatc tcttgtctct gatctcaaga
     3421 ataatcaata gttagcgatc aaacgtcaga gaagagagat cagaggtcag gcaaggttct
     3481 ttcctgatct ctgatctctc gtccctgatc tctcaattca taaggagcac accatgccaa
     3541 atcccaaacg gagacactcg aaagcccgca cgcgcacgcg ccgcgcgcac gacttcctcg
     3601 aagcgaagtc gctctcgacg tgcccgcagt gtcacgaagc gaagatgccg caccgcgttt
     3661 gtccgaaatg cgggttttac aaaggccgcg aagtcatcgc cgtcaaagac tcgttctaaa
     3721 ctccacctgc gatgagtgac gtctgtatcg ccctcgacgc catgggcggc gatcatgcgc
     3781 cggccgaggt ggtgaagggc gcggtcctgg cggcggccga atttgcggtc gagatcattc
     3841 tcgtcggcca ggaagacgtc gtgcggcgcg agctcgccgc tgccggcggc acgccgcgca
     3901 acatcagcgt gttcgacgcg cgcgaaatcg tcacgatgga ggacaccgcc ctggcgccgt
     3961 tgcgcaggaa gcggaactcg tccgttcgcg tctgcgcgaa tctggtggcc gaggggaagg
     4021 ccaacgcctt cgtctccgcg ggtaacaccg gcgcgacctg gacctcggcg cgcgccgtca
     4081 tgggcatgat cgaaggggtg agccgtcccg cgctcgccgc ggtcgtgccg agcctgaagg
     4141 gtcacacgct cctgctcgac gtcggggcga acgtcgatgc caaaccgaat catcttcgcg
     4201 agtttgccgt catgggccac ttctacgcgc agatgctctt cgatgtcgtt gcgccgcgcg
     4261 tcgggttgct ctcgatcggc gaagaggaag gcaaaggcaa cgagctgacg aaggagacgt
     4321 accgggtcct gaaggaaacg ggactcaact tcatcggcaa cgccgaaggg cgcgacgtct
     4381 tcaacggcaa cgcggacgtc gtcgtctgcg acggcttcat cggcaacgtc gttctcaagg
     4441 cgtccgaggc gctgggcgag ttcgtgtcga cgacgctgcg ccaggagatg atgaagacgc
     4501 tgccgcggaa gatcggcggc atgctggcga agagcgcgtt cgagggcctg aagaagcgga
     4561 tggactactc cgagtacggt ggcgcgccgc tgctgggcgt caagggcgga tgcatcgtct
     4621 gccacggccg ctcgaatgcc aaggccatca agaacgccat ccgcgtggcg cgcaacttcg
     4681 ccatcaaccg gatcgacgaa aagatcatgt cgaaggtcaa cgatcttcac aaacgagagc
     4741 acgacagcag cgttttggcc acacaataga gatcatgcaa agccgtccgg cgcgtctcct
     4801 tctcttcgcc atcacgatca cgggctgcgc aacgcactcc gcgaatctgc cgcccgagcc
     4861 gaacgtggtc gagctgcaca cggttccggc gcacgcatcg gtgagtctcg actgcggcgc
     4921 cggaacggtc gcccacggca ccacgccgat gcgcgtcgat gttccgcgct accccgagcg
     4981 ctgcacgatc gacgtcaccg cgaatggcta ccgcccgctg aacatgcgtt tcgaccgcgg
     5041 cctggtgctc aaggaaggtg tgccgctgcg cgtcgaagcg agccacacgc tcgaccggac
     5101 tgccacgacc ctcgacatga tcttgttccc gctgcgcaac ctcgccgaca ggatcgagaa
     5161 cgctgcatcg agctcgggaa gcgcggatta ccggctggag atcaagttga tgccggtggg
     5221 tcagtagccc gcattagccc tgcattagcc cgcatttctc acacccttat caccagctcc
     5281 ttccaatcga gcgccttctc cccccgcgct ttttgcgggg ggagaaggtg gcgaagccgg
     5341 atgagggggg cgttacaaga taggataacc acgttgttcg cgagcggtga cccgattgaa
     5401 aaggcatccg ttcccagtgc ttgggcaatc aacgttgacg cgacaatcaa acgccccctc
     5461 atccgccttc ggcaccttct cccccactga aaagcgtggg ggagaaggcg ctcgatggga
     5521 aggagtcgct gaagggtgtg agaaatgcgg gttagcgccg gacgccccag gttcggagat
     5581 ggtctctttc gaatcatcga cggtgtgttt cgcacccacg atgaagtgtt ctgcacccgc
     5641 agcgacttgt tctgcatccc cggcgacttg ttctgcaccc gcagcgactt gttctgcacc
     5701 cccggt
//
LOCUS       DATLVW010000607        12256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_315830, whole genome shotgun sequence.
ACCESSION   DATLVW010000607 DATLVW010000000
VERSION     DATLVW010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315830"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1948
                     /locus_tag="VNN25_17985"
     CDS             317..1948
                     /locus_tag="VNN25_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXH93477.1"
                     /translation="MISLSKISKQSGRQLVFVDASFQLNPGERAGLVGPNGSGKTTLF
                     RMIVGEESADDGDVSVPKKITIGYFRQDIEEMSGRSVLDEAIAGSGRLGDLHHELEAL
                     QNAMSDPDRLDEMDKILDRFGHVQEDYSHLGGYELEARAREVLHGLGFEDDRIDGDVG
                     ALSGGWKMRVAMAKVLLGRPDVLLMDEPTNHLDIESIIWLEDFLKRYEGSLLMTSHDR
                     EFMNRVVTKIVEIDEGDLVTYSGNYEFYERERAIRETNREAAYARQQSMLAKEQRFID
                     RFAAHAAKAAQVQSRVKALDKIEKLEPPKKRKTMKFDFRKPSRSGDDVVTLKDVSKAY
                     GKRSIYEDFDFLIRRGERWCVMGKNGAGKSTLLKMVSGAVEPDGGNVRLGASLKMGYF
                     SQQAFDVLDPNLTVWEQIQKDFPLESIGTLRSLLGAFQFSGDEVEKKIRSLSGGERSR
                     LVLARMLLDPPNFLVLDEPTNHLDLATKEMLVEALHDFEGTMLFVSHDRMFLRGLSNR
                     VLELPEERGAAPRVFGGSYVEYVSDSGHEAPGVHT"
     gene            complement(2598..4982)
                     /locus_tag="VNN25_17990"
     CDS             complement(2598..4982)
                     /locus_tag="VNN25_17990"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HXH93478.1"
                     /translation="MRAAIRIAFIALTLAASTAGAATFLVPPDRTLVTASNAIVVATA
                     GESHGRWAPGGWIETVTELRVDEAIKGEIGTGETIHVTELGGSVSGISYIVAGSPKYA
                     PGERVLLFLETNDRGEWVAKNMVVGKFTPAQDTSGRQLLLREAGEIVGWNVDGSPHHE
                     PVRLEAEFLRFVRAVAQGRDAAEDYIVSDPKPLRKNIISEATASAFAPSSYLIQSGGL
                     GIRWPSFPNTVVFLSHGTQPGALNGGITAMQRAFAAWTSDPGSNIVYGYGGTTPIAST
                     GFGSNVNDGVNTVQFNDPANEIPGSFTPTNGATLAIGGAWTGNATHQANGEQYRTIVE
                     ADLVVQDGISGPGLTGNGFDHVLTHELGHTLGLRHSDKNAADTANCSAPLDCSTTAIM
                     DSGVAFDSDPIGSALQAWDIAAIDAVYGSGVVTPPACNPPRINTQPAPTIDVGTTAVT
                     FTVTASGDTPLQYQWYTGTRGNTNAPVTGANSASFTVKPAVTTGYWVRITNGCDPPAD
                     SITAYAIVNNCPPVTIASLSGDVTIFEGKSTPLAVAASGGTVSYQWYAGTSGTTTSPI
                     PNATGATMTVSPAQTTSYWVRASNTCGSSTDSSTVTVTIVPCTAPKVVVQPASSNIVT
                     GNGATLFAAVTGTQPISYQWYEGTFPDTTHPANSGTSATLAVPSLVASTSYWLHVSSV
                     CGETDSTTARLTIVSTCTPPAIAVQPQNQSVASGSAAIVSISATGSSLSYQWYQGGFL
                     DFTHPLGGNAPAVLTPAITTTAQFWVRITSPCGSVDSTVATVSVAVQPRRRAAK"
     gene            complement(5049..5771)
                     /locus_tag="VNN25_17995"
     CDS             complement(5049..5771)
                     /locus_tag="VNN25_17995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXH93479.1"
                     /translation="MLAAILIAIAAFFAGAVSAVAGFGIGSILTPLLGLTIGVKLAVA
                     AAAIPHFIGNALRLWTLRDKVDKHVLKTFGLMSGAGALTGALLYAAVATSALKLVFAI
                     VLIIVGAFGVTGWSDHLRFGRRGAWLAGGISGLLGGLVGNQGGIRAAAMLALDVRKEV
                     FVATAVAIALVVDGARLPVYAFSTGRQLLTVWPMLAGASVAVILGTFFGKSLLGRVPE
                     SVFRRLVAALLIALGVAMLVSR"
     gene            complement(5771..6160)
                     /locus_tag="VNN25_18000"
     CDS             complement(5771..6160)
                     /locus_tag="VNN25_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXH93480.1"
                     /translation="MPDLVTLFQALSDATRLRLLSLMAAGDVCVCYFVEILDETQPKI
                     SRHLAYLRRAGLVSARREGKWVHYGLVKPSDPAAAAVFEKVLEVITNDRRMQRDRAAL
                     EKVCCAVRLPAALQNAPKPALVRARTN"
     gene            complement(6384..6938)
                     /locus_tag="VNN25_18005"
     CDS             complement(6384..6938)
                     /locus_tag="VNN25_18005"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXH93481.1"
                     /translation="MTILNRAESFLVIVDVQEKLMPVIDDHESVERNLDRLIRGGHIL
                     DIRAVVTEQYTKGLGHTVPRLRTTLEATFGYEPIEKLTFSAFGCVEFVAVVRNLHRKQ
                     AIVAGIETHVCVYQTVRDLLANGFDVTVIADAVSSRTQANRETALRRMVSAGAKLSST
                     EMALFELTGVSGTDEFREIARLVR"
     gene            complement(6953..7870)
                     /locus_tag="VNN25_18010"
     CDS             complement(6953..7870)
                     /locus_tag="VNN25_18010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXH93482.1"
                     /translation="MKPVVVLTAQYPAIVREILAGLCDFVEHPSENGRSEDDMITILS
                     EADAAITLLSDPLTRNVLEANPGLHMIANYAVGYNNVDLEAARELGVIVTNTPGVLTE
                     ATADLTMGLILAVMRRIIEGDREVRTTGRCVWEPLHLLGSGLTGKRLGIIGIGRIGSA
                     VATRAAAFGMEVIGVRRGESLDELLATSDIVSIHAPLTGQTRHLMNGTTLHAMKRGAF
                     LINTARGPLVDETALCDALDDGHLAGAGLDVYEDEPSVSPRLIAMPNVVLLPHIGSAT
                     EETRAAMARIAATDVALFLRGLKPQNIIT"
     gene            7912..8082
                     /locus_tag="VNN25_18015"
     CDS             7912..8082
                     /locus_tag="VNN25_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93483.1"
                     /translation="MSALLGRLFMLIGMIILPIGLLTGLLKDNVSLEVRLLFIGGAFF
                     LIGWLIAKRPSE"
     gene            complement(8097..8297)
                     /locus_tag="VNN25_18020"
     CDS             complement(8097..8297)
                     /locus_tag="VNN25_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93484.1"
                     /translation="MPANPTKTNAPITKKNHAAEPAADEMRSHYDFDYSKSRPNRFAS
                     RPKVGRVRSVVLDTKNDAGDVE"
     gene            complement(8334..8897)
                     /locus_tag="VNN25_18025"
     CDS             complement(8334..8897)
                     /locus_tag="VNN25_18025"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH93485.1"
                     /translation="MQQAQRHYTIDEYFAVEAMSPVRNEYFDGEILAMAGGSPNHNRI
                     SRNVLLALEPKLRDGRCESFGSDTRILTASGLYTYPDAVVICGPIILSGDMLESITNP
                     TVLVEVLSPSTRAYDRGEKFELYRSIPAFRDYVLIEQSAVDVEHRWRAGDQWSSARKT
                     ALDDVIQLNSIEVDLKVADLYARVQLT"
     gene            complement(8952..10991)
                     /locus_tag="VNN25_18030"
     CDS             complement(8952..10991)
                     /locus_tag="VNN25_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH93486.1"
                     /translation="MRTTALALLLGVACTTATPPPAPQPVPPPAPPSAHGFTIEEEVR
                     VLQLEDRREYDEAFTNAWLANPNALHRARIALALGRIGPHTFVDTNGNGMRDAGERQA
                     GVAQLVALVSDPDANVRATAAFALGQIGDATSVDALLQFAADHDGDVAGEAVEALSKL
                     AAHVPLARYAAFTGAQIPEGIRARAVRFLFRFKSDEASAIAADLLASPSPRIREEATY
                     ALARRAYAGARPRLELLVNEPGHQTRANVMSALGRIGSAESLPLLIDALRDQHPWVRT
                     NAVVAISRLAAKDRKSIERPQLASDAKRVMELLDDPDAGTRASSIDTLGYYAARDDAA
                     RKRLLDVAANGSRWDREIAAGAIAKNLGDETLLPADLTSWAKVRVLEAATPISDAVRA
                     RYARDSETLVRAQALGTIADDKIDANLPMIRKALDDYDVIVRGYAIGAFAKSHDPNKL
                     DTLQGAERRARSDGQNDARLAAISALSEIDYPDRAAVLRVWLADPDPVVRRIAADAIE
                     QKLKMPRPQFTPLPVTRNDYTQIVEWSRQPHTASIHMTRGIIQIALLTKDAPITTWNF
                     AKLARAKYFDDSSFMRVVPNFVIQGGDPRNDMEGGPGYTIRDEINLQKYTRAAVGMAL
                     SGPDTGGSQFFITHSPQPHLDGGYTIFGRVIAGMTAVVDPTERGDKVETIAIDEK"
     gene            11071..11742
                     /gene="ccmA"
                     /locus_tag="VNN25_18035"
     CDS             11071..11742
                     /gene="ccmA"
                     /locus_tag="VNN25_18035"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HXH93487.1"
                     /translation="MEAPPGIDAHNLARRYGRRWALADVSFSLAAGSVMMVAGPNGSG
                     KSTLLRTLATAIRPDAGSASIGGFDLVREREDVRRLVALLSHSSYLYDSLTAHENLAI
                     AADHLGRSRSGIPALLERVALGRRADDPVATFSAGMRKRLSFARVLLQEPRVVFLDEP
                     YGQLDPEGFALVDDVVHELKAGGATVVIATHQLPRASGIADEALLLDSGRVVWRGKAS
                     EVGGR"
     gene            12023..>12256
                     /locus_tag="VNN25_18040"
     CDS             12023..>12256
                     /locus_tag="VNN25_18040"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXH93488.1"
                     /translation="MIAALRKELLLQWRSKAQLMAIFVFGAAALLLFSFAVGPDAEAL
                     RKFSAGFLWLGLLLSSTLTLAESFHAEMENRAIE"
BASE COUNT         2207 a         3728 c         4027 g         2294 t
ORIGIN      
        1 cttcttggct gttgtggtga gagagatcac cggttaggaa ccctgtcccg acacgctcgg
       61 aacaggtata cgtacggaac gactcgcaca ttgcgagggg cactggcaat acatccgtct
      121 gtgatgaccg gcgcagccta cgcccattcg taactccggc gcaaccggaa tttgaaggca
      181 gccggactcg ggcgtgggcc gcctcacgcg aagccgcgaa gacgcgaaac gagaacaagc
      241 ctgcttcgcg tcttcgcgcc ttcgcgtgag acagaagcga aatgccgccc cggccccccg
      301 cgttgtctcc acgctcatga tcagcctcag caagatctcc aagcagtccg gccggcagct
      361 cgtctttgtc gacgcctcgt ttcagctcaa ccccggcgag cgcgccggac tggtcgggcc
      421 gaacggctcc ggcaagacga ccctctttcg catgatcgtc ggcgaagaat cggccgacga
      481 cggcgacgtc tccgttccga agaaaatcac catcggctac ttccgccagg acatcgaaga
      541 aatgtccggc cgctccgtgc tcgacgaagc gatcgccggc agcggccgcc tcggcgatct
      601 tcaccacgag ctcgaagcgc tgcagaacgc gatgtccgat cccgaccgtc tggatgaaat
      661 ggacaagatc cttgaccgct tcggccacgt ccaggaggac tactcccacc tcggtgggta
      721 cgagctcgaa gcacgcgccc gcgaggttct ccacggtctc ggcttcgagg atgaccgcat
      781 cgacggcgac gtcggtgcgc tcagcggcgg ctggaagatg cgggtggcca tggccaaggt
      841 tctcctgggg cggcccgatg tgctgctgat ggacgagccg accaaccatc tcgacatcga
      901 gtcgatcatc tggctcgaag acttcctcaa gcgatacgaa ggctcgctgc tgatgacgtc
      961 gcacgaccgc gagttcatga accgcgtcgt cacgaagatc gtcgagatcg acgagggcga
     1021 cctcgtcacc tactccggca actacgagtt ctacgagcgg gagcgcgcca tccgcgagac
     1081 caaccgcgag gccgcctacg cgcggcagca atcgatgctg gccaaggagc agcgcttcat
     1141 cgaccgcttc gcggcgcacg cggcgaaagc ggcacaggtg cagagccgcg tgaaggcgct
     1201 cgacaagatc gagaagctcg agccgccgaa gaagcggaag acgatgaagt tcgatttccg
     1261 caaaccgtcg cgatcgggcg acgacgtcgt cacgctgaag gacgtttcga aggcgtacgg
     1321 caagcgatcc atctacgagg acttcgactt tctcatccgc cgcggcgagc gctggtgcgt
     1381 gatgggaaaa aacggcgccg gcaagtcgac gctgctgaag atggtctcgg gcgcggtcga
     1441 gccggacggc ggcaacgtcc gcctcggcgc atcgctgaag atgggctact tctcgcagca
     1501 ggccttcgac gtgctcgatc cgaatctcac cgtgtgggaa cagatccaga aagactttcc
     1561 gctcgaatcg atcggcacgc tgcgcagcct cctcggcgcg ttccagttct ccggcgacga
     1621 ggtggagaaa aagatccgct cgctctccgg cggcgagcgc tcacgcctcg tgctggcccg
     1681 gatgctcctc gatccaccga acttcctcgt cctcgacgag ccgacgaacc atctcgacct
     1741 ggccacgaag gagatgctcg tcgaagcgct tcacgatttc gaagggacga tgctcttcgt
     1801 ctcgcacgac cgcatgttcc tgcgcggcct ctccaatcgg gtgctcgagc tgcccgaaga
     1861 acgcggcgcc gccccacgcg tgttcggagg ctcctacgtc gaatacgtca gcgacagcgg
     1921 ccacgaagcg ccgggagtgc atacgtagtt ttcgggggtg gggtgtgggg ggtggggagt
     1981 gggaatgtga tgcgaccagc caggtgacct ctttcgagga cgtccggagc caatttcggt
     2041 agtccttttg acgatttcag gagtcgaaac cactaccgaa atggtcccga cctaatttca
     2101 gtagtcaaaa cgaccaccga aattagtctc gacctaaatt gagcagtcaa aacgaccacc
     2161 gaaattggtc ccgacgtaat ttgagtagtc aaaacgacca ccgaaattgg tctcgaccta
     2221 atttgagtag tcaaaacgac caccgaaatt ggtctcgacc taatttgagt agtcaaaacg
     2281 accaccgaaa ttgtccatgg cctaatttca gtagtcaaaa cgactaccga aagtgtccat
     2341 ggcactattt cacgagtcaa aacgactacc gaaattggtc ccggcctaat ttcagtagtc
     2401 aaaacgacta ccgaaagtgt ccatggcact atttcacgag tcaaaacccc tactgaaatt
     2461 ggtcttgacc caatttcact aggccttttg aagcggattg gagggtcagg tctaaagtct
     2521 aaagtgccga ggacgatcgt cgttaaggcg atcagtcgtc gtcggcactt tagactttag
     2581 acctgaccca atccgtgcta cttcgccgct cttcgccgcg gctgtacggc gacggacacg
     2641 gttgcgacgg tgctgtcgac gctgccgcag gggctggtaa tgcgcaccca gaactgcgcc
     2701 gttgtggtga ttgcgggcgt cagcaccgcg ggggcgttgc cgccgagggg gtgggtgaaa
     2761 tcgaggaagc caccctggta ccactgatac gagagcgatg agcccgttgc gctgatcgat
     2821 acaattgcgg ccgatcccga ggccacgctc tgattttgcg gttggacggc gatcgccggc
     2881 ggcgtgcagg tagatacgat cgtgagccgc gcggtggtgc tgtcagtctc gccgcatacg
     2941 ctcgagacgt gcagccagta cgaagtggag gcgacgagcg acggcacggc cagcgtggcc
     3001 gaggtgccgc tgttggctgg atgcgtcgtg tccgggaacg ttccttcgta ccactggtag
     3061 gagatcggct gcgttccggt cactgccgcg aagagcgtcg ctccgttgcc ggtgacgata
     3121 ttgctgcttg ccggctgcac gacgaccttc ggagctgtgc acgggacgat ggtgaccgtc
     3181 accgtgctgc tgtcggtact cgatccacag gtgttcgatg cccgcaccca gtagctggtc
     3241 gtctgcgccg gtgaaacggt catcgtcgcg ccggttgcgt tggggatcgg cgacgtcgtc
     3301 gtgccgctcg tgcctgcgta ccattgatac gaaacggttc cgccgctggc agcgaccgcg
     3361 agcggcgtcg acttcccttc gaaaatcgtg acgtcgcccg acagcgaagc gatcgtgacc
     3421 ggcggacaat tgttgacgat cgcgtaggca gtgatgctgt ccgcgggagg atcgcatcca
     3481 ttcgtgatgc gaacccagta gccggtggtc accgccggct tcacggtgaa gctcgccgag
     3541 ttcgcgcccg tcaccggcgc attcgtattt cctcgggtgc ctgtgtacca ctggtattgc
     3601 agcggtgtgt cacccgaggc ggttacggtg aaggtgacag ccgtagtgcc gacgtcgatt
     3661 gtcggcgccg gttgcgtgtt gatccgcggc ggattgcagg ccggcggtgt gaccacgccg
     3721 gagccgtaca cggcatcgat cgctgcgatg tcccacgcct gcagagccga accgatcggg
     3781 tcggagtcga aggcgacgcc ggagtccatg atcgccgttg ttgaacagtc gagcggcgcg
     3841 gagcagttgg ccgtgtcggc agcgttcttg tcggaatgac gcagcccgag tgtgtgcccg
     3901 agctcgtgcg tgaggacgtg atcgaatcca ttgccggtca gacccgggcc gctgatgccg
     3961 tcctgcacga cgaggtcggc ttcgacgatc gtgcggtact gctcgccgtt tgcctgatgc
     4021 gtggcgttcc cggtccaggc gcccccgatc gcgagcgtgg cgccgttggt tggcgtgaac
     4081 gagccaggaa tctcgttcgc aggatcgttg aactgcacgg tgttcacgcc gtcgttgacg
     4141 ttcgatccga agccggtgct ggcgatcggc gtcgtgccgc cgtatccgta gacgatgttg
     4201 gagcctgggt cgctcgtcca cgccgcgaag gcgcgctgca tcgccgtgat gccgccgttc
     4261 agggcgcctg gctgcgtgcc gtggctgagg aagacgaccg tgttggggaa gctcggccaa
     4321 cggatgccta gccctcccga ttgaatcaga tacgagcttg gcgcaaatgc cgacgccgtc
     4381 gcctcgctga tgatgttctt ccgcaacggt ttcggatcgc tgacgatgta gtcctccgcc
     4441 gcatcacgcc cttgcgcaac cgcgcgcacg aagcgcagga actcggcttc gagccgcacc
     4501 ggctcgtgat gcggtgagcc gtcgacgttc cagcccacga tctcgcccgc ttcacggagg
     4561 aggagctgtc gcccgcttgt gtcctgggcc ggcgtgaact tcccgaccac catgttcttc
     4621 gccacccatt cgccgcgatc gttcgtctcc agaaagagca gcaccctctc gcccggcgcg
     4681 tacttcgggc ttccggcgac gatgtagctg atcccgctga cggaaccacc gagctcggtg
     4741 acgtgaatcg tctcgccggt accgatctca cctttgattg cctcgtcgac acgaagctcc
     4801 gtcacggtct cgatccatcc gccgggggcc caacgtccgt gcgactcccc ggcagtggcg
     4861 acgacgatcg cattcgacgc agtgacgagt gtgcggtccg gcgggacgag gaacgtcgct
     4921 gcaccggcgg tgctcgccgc aagcgtgaga gcgatgaaag cgattcggat ggcagcgcgc
     4981 atcaggttga cgatcgtaac gacgaccgga agtcatccgt gatgacagcg gtcacggtgc
     5041 tgcgaatttc atcgcgaaac gagcatggcc acgccgaggg cgatcagaag ggcggcgacg
     5101 aggcggcgaa aaacgctttc cggcacgcgc ccgaggagag actttccgaa aaaggtgccg
     5161 aggatgacgg cgacgctggc gccggcgagc atcggccaga cggtcaggag ctgccgtccc
     5221 gtgctgaacg cgtaaaccgg caggcgcgcg ccatcgacga cgagggcgat ggcgacggcg
     5281 gtggcgacga agacctcttt gcgcacgtca agcgcgagca tcgccgcggc gcggatgccg
     5341 ccctggttgc cgaccagtcc gcccagcagc ccggagatgc cgcccgccag ccacgcgccg
     5401 cggcggccga agcggaggtg atcgctccaa ccggtaaccc caaaggctcc gacgatgatc
     5461 agcacgatgg cgaagacgag tttcagagcg ctcgttgcca cggcagcgta gagcagggca
     5521 ccagtcagcg cgcccgcgcc gctcatcagg ccgaatgtct tgagcacgtg tttgtcgact
     5581 ttgtcgcgca atgtccagag acgaagcgca ttgccgatga agtgcgggat cgccgcggcg
     5641 gccaccgcca gtttgacgcc gatggtcagg ccgagaagcg gcgtgaggat gctgccaatg
     5701 ccgaagccgg ccacagccga caccgcaccg gcgaagaacg ccgcgatggc gatgaggatc
     5761 gccgccagca tcagttcgtc cgcgccctca cgagagccgg tttcggtgcg ttctgcaacg
     5821 cggcgggcag ccggacggcg caacagacct tctccagcgc ggcgcgatcg cgctgcatcc
     5881 ggcggtcgtt ggtgatgacc tcgagaacct tctcgaagac cgcggcagcc gcgggatcgg
     5941 acggtttcac cagcccgtaa tgcacccact tcccctcgcg ccgcgccgac accagcccgg
     6001 cccgccgcag ataggcgaga tgccgcgaga tcttcggctg cgtctcatcg aggatctcga
     6061 cgaaatagca gacgcagaca tcgcccgccg ccatcaggct caagagccgg agccgcgtag
     6121 cgtcgctgag cgcctggaag agggtgacga ggtcgggcat cggcgggatg ataattgaca
     6181 attgataatt gacaattgac aacggggatc aagtcagaga tcagaaggtc agagatcaga
     6241 gggtcagaga tcagaagatc agagatcaga agatcagaga tcagaagatc agaacgtcag
     6301 agatcagaag attggaagat tagaagatca gagacgcccg tgatcagcgg ctcgttgtca
     6361 attgtccatt gtcaattgtc aattcacctc acgagccgcg cgatctcccg gaactcgtct
     6421 gtcccggaaa ctcccgtcag ctcgaacagc gccatctcgg tcgagctcag tttggcgccg
     6481 gcgctgacca tgcggcggag ggcggtctcg cgattggctt gcgtgcggga ggagacggcg
     6541 tcggcgatga cggtgacgtc gaatccgttc gcgagaaggt cgcggacggt ctggtaaacg
     6601 cagacgtgcg tctcgatccc ggcgacgatg gcttgtttcc ggtgcagatt gcggacgacg
     6661 gcgacaaact cgacacatcc gaaggccgag aacgtcaact tctcgatcgg ttcatagccg
     6721 aacgttgcct cgagcgtggt gcggagccgc ggcacggtgt ggccgagtcc tttggtgtat
     6781 tgctcggtga cgacggcgcg gatgtcgagg atgtgaccgc cgcggatgag gcggtcgagg
     6841 ttgcgctcca cgctctcgtg gtcgtcgatg accggcatga gcttctcctg cacatcgacg
     6901 atgacgagaa aggactccgc acgattgagg atggtcatga tcgttcgctc actcaggtga
     6961 tgatgttctg cggcttcagt ccgcgcaaga agagagccac gtcggtggcg gcgatgcggg
     7021 ccatggcggc tcgggtttcc tccgtcgccg agccgatgtg cggcaggagg acgacgttcg
     7081 gcatcgcgat caggcgcgga ctgacggagg gctcgtcttc gtacacatca agtccggcgc
     7141 cggcgaggtg gccgtcgtcg agcgcgtcgc agagagccgt ttcgtcgacc agcggcccgc
     7201 gcgcggtgtt gatcaggaat gcgccgcgct tcatcgcgtg gagagtggtc ccattcatca
     7261 ggtgacgggt ctggccggtc agcggcgcgt ggatcgatac gatgtcgctc gtcgcgagaa
     7321 gctcgtcgag agactcgccg cgccggacgc cgatgacctc catcccgaat gccgctgcgc
     7381 gcgtcgccac cgcgctgccg atgcggccaa tgccgatgat cccgagccgt ttgcccgtga
     7441 gcccgctgcc gaggagatgc agcggctccc agacgcaccg gccggtggtg cgcacttcgc
     7501 ggtcgccttc gatgatgcgt cgcatcaccg cgaggatgag ccccatcgtc agatccgcgg
     7561 tcgcttccgt cagtacgccg ggagtgttcg tgacgatgac gcccagctcg cgcgcggcct
     7621 cgaggtcgac gttgttgtag ccgaccgcgt agttggcaat catgtgcagg ccgggattcg
     7681 cttcgagaac attgcgcgtg agcggatcgc tgagcagcgt gatggccgca tcggcttcgc
     7741 tcaaaatcgt gatcatgtcg tcctctgagc gtccgttttc gctgggatgc tcgacgaagt
     7801 cgcagagtcc ggccaggatt tcgcggacga tggccgggta ctgcgccgtg aggacgacga
     7861 ctggcttcac gccgcgaact gtagcagcgc ggcgggcgtt acacttcccc gatgtccgcg
     7921 ctcctcggcc gcctcttcat gctcatcggc atgatcatcc ttccgatcgg tttgctcact
     7981 ggtctgctga aggacaacgt atcgctggaa gtgcgcctgt tgttcatcgg cggcgcgttc
     8041 ttcctgatcg gctggctgat cgcgaagcgg ccgtcggagt agggggacct tctggtttac
     8101 tccacatcgc cggcgtcgtt ctttgtatcg agaacaacgg agcgaacacg tccgaccttc
     8161 ggccgggaag cgaaacgatt cgggcgcgat ttgctgtaat caaagtcgta atgcgatcgc
     8221 atctcgtcgg cggctggttc agcggcgtga ttcttcttcg tgatgggtgc gttcgtcttt
     8281 gtcgggtttg cgggcattga tgattcgaat ctcgccaggc tcgggtaatc gccctaggtc
     8341 agttgcacac gggcatatag atcggcgacc ttgagatcca cttcgatgct gttcagttga
     8401 atgacatcat cgagcgccgt cttccgggca ctcgaccatt gatcaccggc gcgccaccgg
     8461 tgctccacat cgacagcgct ttgctcgatg agcacatagt cgcgaaaggc cgggatcgat
     8521 cgatagagct caaacttctc gccgcgatcg taggctcggg tcgatggcga gagcacttcg
     8581 accagcacgg tcggattggt gatcgattcg agcatgtcgc cgctgaggat gatcggtcca
     8641 cagataacga cggcatcggg gtacgtatag agcccggagg ccgtgaggat tcgcgtatcc
     8701 gacccaaacg attcgcagcg tccgtcgcgc agcttcggtt cgagcgccag aagaacgttt
     8761 ctggagatcc ggttgtggtt cggtgatccg ccagccatcg ccaggatctc gccgtcgaaa
     8821 tactcgttgc gaaccggcga catggcttca actgcgaagt actcgtcgat ggtgtagtga
     8881 cgttgtgctt gctgcattac ggcctcatgt tactccggtg gagcagggcg agcggcgctg
     8941 gtgacagccc ctcacttctc gtcgatcgcg atcgtctcta ctttgtcgcc gcgctcggtt
     9001 gggtcgacga cggcggtcat gcccgcgatg acgcggccga agatcgtgta gccgccgtcg
     9061 aggtgcggct gtggcgagtg cgtgatgaag aactgagagc cgccggtgtc gggaccggag
     9121 agggccatgc ccaccgcggc gcgcgtgtac ttctgaaggt tgatctcgtc gcggatggtg
     9181 tagcccggcc cgccttccat gtcgttgcgg ggatcgccgc cctggatgac gaagttgggg
     9241 acgacgcgca tgaacgagct gtcatcgaag tacttcgcgc gcgccagttt cgcaaagttc
     9301 catgtcgtga tcggtgcgtc cttcgtgaga agcgcgatct ggatgattcc gcgggtcatg
     9361 tggatcgacg cggtgtgcgg ttgccgcgac cactctacga tctgcgtgta gtcgttgcgc
     9421 gtgaccggaa ggggcgtgaa ctgcggccgc ggcattttaa gcttctgctc gatcgcatcc
     9481 gcggcgatgc ggcgcacgac tggatcggga tcagctagcc atacgcgaag aaccgccgcc
     9541 cggtccgggt agtcgatctc cgaaagcgcg ctgatcgcag cgaggcgggc gtcgttctgc
     9601 ccgtcgctgc gcgcgcgccg ttctgcgcct tgcagcgtat ccagcttgtt cgggtcatgt
     9661 gatttcgcga aggcgccgat cgcgtagccg cggacgatga cgtcgtagtc gtcgagcgct
     9721 ttgcgaatca tcggaagatt ggcgtcgatc ttgtcgtccg cgatcgtccc cagcgcctgc
     9781 gcgcgcacga gcgtttccga atcgcgggcg tatctggcac ggacggcgtc ggagattggc
     9841 gtggccgctt cgagtacacg cacttttgcc caactggtga ggtctgcagg caacagtgtt
     9901 tcgtcgccga ggtttttggc gatcgcgccg gcggcgatct cgcggtccca gcgcgagccg
     9961 tttgcagcga cgtcgagcag ccgtttgcgc gcggcgtcgt cacgcgctgc gtagtaaccc
    10021 aatgtatcga tcgatgaggc gcgcgtgccg gcgtcgggat cgtcgagcaa ctccatcacg
    10081 cgcttcgcat cgcttgcgag ttgcggccgc tcgatcgact tccggtcctt tgcggcgagg
    10141 cggctgatgg caacgacggc attggtgcgc acccagggat gttgatcgcg caacgcatcg
    10201 atcagaagag gaagcgattc cgccgatccg atcctgccga gggcggacat gacgttggcg
    10261 cgcgtctggt gacctggctc gttgacgagg agctccaggc gcggccgcgc gccggcatac
    10321 gcgcgccgcg ccagcgcgta ggtggcttcc tcgcgaatgc gcggcgaggg agaagcgagc
    10381 aaatcggcgg caatcgccga tgcttcatcg gacttgaagc ggaagaggaa acggacagcg
    10441 cgcgcgcgaa tgccttccgg gatctgcgcg ccggtgaatg cggcgtaacg cgcaagaggc
    10501 acgtgcgccg cgagcttgga cagcgcctcg actgcctccc cggcgacgtc gccatcatga
    10561 tcggcggcga actggaggag cgcatcgacc gaggtggcgt cgccgatctg cccgagggcg
    10621 aaggcggcag tggcgcgaac gttcgcatcg ggatcgctga cgagtgccac gagctgtgcg
    10681 accccggcct ggcgttctcc ggcgtcgcgc atgccgttgc cgttcgtatc gacaaacgta
    10741 tgcggtccga ttcgccccag cgccagcgcg atccgcgcgc gatgcagcgc gttggggttt
    10801 gcgagccatg cgttggtgaa ggcctcgtcg tactcacggc ggtcttccaa ctgcagcacg
    10861 cgcacctctt cctcgatcgt gaagccgtga gcggatggag gagcgggcgg cgggacgggt
    10921 tggggcgcgg gcggcggagt ggcggtggtg caggctacgc ctaggagcag ggcgagtgcg
    10981 gttgtgcgca tcgcgagaaa tgatagcgcc gccgctcctc ttcgcgcatt gcttgccaca
    11041 cgcgcgaacc tgacacagac tgcgcgctgc atggaagccc ctcccggaat cgacgcacac
    11101 aatctggccc gccgctacgg gaggcggtgg gccctggcag acgtgtcgtt ctctctggcc
    11161 gcgggaagcg tgatgatggt ggccggcccg aacggttcgg gcaagtcgac cttgctccgt
    11221 acgcttgcca cggccatccg tccggacgca ggctcggcaa gcatcggcgg cttcgatctc
    11281 gtccgcgagc gcgaagacgt tcgccggctg gtcgcgcttc tcagccattc gtcgtatctc
    11341 tacgattcgc tcaccgccca cgagaacctg gccatcgccg ccgaccacct cgggcgctca
    11401 cgctccggca tccccgccct cctcgaacgc gtggcactcg gacgccgcgc agatgatccc
    11461 gtagccacgt tttcggccgg catgcgcaag cgcttgtcgt tcgcccgcgt tctgctgcag
    11521 gaaccacggg tcgtctttct cgacgagccc tacggccagc tcgatcccga gggcttcgcg
    11581 ctggtcgatg atgtcgtgca tgagctcaaa gccggcggag cgaccgtcgt gattgccacg
    11641 caccagcttc cgcgcgcatc gggcatcgcc gacgaagccc tgctgctgga cagcgggcgg
    11701 gtggtgtggc gggggaaggc gagcgaggtg ggggggcgat gagggacatc gtgagccggg
    11761 gaagcctctt cgttctcttt tccgtgcaaa aaaacgacgg tgtcatcctg agccgccaac
    11821 ggaaggtcga aggatctcaa attgaacggc aggtgccatg tgcatcgtat tctgagatcc
    11881 ttcgccgtcc tacgccgcgc gaccatgctg tgagaaggcc aggcgaatcg cggcgcggct
    11941 ccggcaggct caggatgaca cctccgattt tctggacctg gactttcgca aacgacgagg
    12001 gtgccgtcgg aaaggtgccc gcttgatcgc cgctcttcgc aaagaacttc tcctccagtg
    12061 gcggagcaag gcgcagctca tggccatctt tgtctttggt gcggctgcgc tgctcctgtt
    12121 tagttttgcg gttggccctg atgccgaggc gttgaggaaa ttctcggcgg ggtttctttg
    12181 gctggggctt ctgctctcgt cgacgctgac tcttgcggag agctttcatg cggagatgga
    12241 gaaccgggcg attgaa
//
LOCUS       DATLVW010000608         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_396265, whole genome shotgun sequence.
ACCESSION   DATLVW010000608 DATLVW010000000
VERSION     DATLVW010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396265"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..681)
                     /locus_tag="VNN25_18045"
     CDS             complement(298..681)
                     /locus_tag="VNN25_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH93489.1"
                     /translation="MRVAFDTNRYVDFCRGVVGTVTLFEEAGMVALPFIVLGELRAGF
                     AHGKRQLENERILRDFLLRDGVRALFADDQTTHHYGALYRQLRKQGTPMPANDVWIAA
                     LVLQHNLVLHSRDRHFDHLPQLARV"
     gene            complement(678..920)
                     /locus_tag="VNN25_18050"
     CDS             complement(678..920)
                     /locus_tag="VNN25_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93490.1"
                     /translation="MQYTIRGIPETLDAALRERARVAGKSLNEVAIEVLAEGAGLTGA
                     PRKRRSADGIAGSWKEDKAFDEAIAAQDRIDEDMWK"
     gene            complement(967..1650)
                     /gene="sdaAB"
                     /locus_tag="VNN25_18055"
     CDS             complement(967..1650)
                     /gene="sdaAB"
                     /locus_tag="VNN25_18055"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HXH93491.1"
                     /translation="MAIRHDRKVSILDVMGPVMVGPSSSHTAGTARLGRVAREILDED
                     PVAVHFYLHPPLGATYRGHGSDFALVGGSIGLSVDDPRIPEAIRIAEQMDVDITFSEE
                     DLGDVHPNTVRIEIRGKTREAEISGSSIGGGVIEVFKINGFQTRFKGDSPTLLLFYRD
                     RPGMISEVTKIIAEEGINIASLSCSRKQRGKDAFMQIDVDSPMSEAALQRIRSVEDVE
                     EARYLDRIP"
     gene            1711..2112
                     /locus_tag="VNN25_18060"
     CDS             1711..2112
                     /locus_tag="VNN25_18060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93492.1"
                     /translation="MPEKPIILVVDDDQPILLLMRSLLKEFGFEAVTAENGNQALAAA
                     RERRPAVVLVDKNMPGMTGAETIQSLRDEPGLERLPILILSGEPVSRSELAGLRADGA
                     VLKPFDVTALVEQIRAFVADGDGAGPQTSRA"
     gene            2152..>3101
                     /locus_tag="VNN25_18065"
     CDS             2152..>3101
                     /locus_tag="VNN25_18065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXH93493.1"
                     /translation="MTDINVDQLVHTAVFGDGDAKADARRTIHMEARRRGAVSSSIYP
                     FYMGFGRNEITRRFTVPAFNIRALTYDAARALFRVAMRHDAGAFIFEIARSEIGYTEQ
                     RPAEYAACVLAAAMAEGFEGPVFIQGDHFQASAKKWATPEGQAAEMKALESLIDEALA
                     AEFYNIDIDTSTLVNLDFPTRDEQQRANYEGTAHLTKYIREREPKGVTVSVGGEIGEV
                     GKYNTHPEEVRAYADGLKRLLGGVTGISKISVQTGTSHGGVPLPDGSIAQAKIDFNVL
                     RETTHICREEYGMAGAVQHGASTLPESVFNHFPESEAVEI"
BASE COUNT          595 a          971 c          950 g          585 t
ORIGIN      
        1 cgcgccgccg ccgcaacgct tcgaggatct gatcgccggg aacggcgtgc tcctgttcgt
       61 cgctctccag aaagacgtcc gcaaggtcgc ggccgccagc gagtttttcg aggtgggcaa
      121 agctttcgat catgggtggc agaacaggaa tcggggcgcg ggctattcgc ggtgtgttgg
      181 agaaccgctt accacagaga cacagaggtc actgagagtc ctctgtgttc tctgtgtctc
      241 tgtggttgcc tttgcttccg aaatcacgat gtgcatccga attgatcacg accctgatca
      301 aacgcgggcc agttgtggaa ggtgatcgaa gtgccggtct cgcgagtgaa gtacgagatt
      361 gtgctgcaga accagcgccg caatccacac gtcgttagct ggcatcggag ttccctgttt
      421 ccggagctgg cggtagagcg caccatagtg atgcgtcgtc tggtcgtccg caaaaagggc
      481 cctgacgcca tccctaagca ggaagtctcg cagaatccgc tcgttctcca gctgacgctt
      541 tccatgcgca aaaccagctc gaagctcacc aagaacgatg aacggcagcg caaccatgcc
      601 tgcttcctcg aaaagcgtca cggttccgac aacgcctcgg cagaaatcaa catatcgatt
      661 cgtgtcgaaa gcgaccctca cttccacata tcctcatcga tccgatcctg cgcagcgatt
      721 gcctcatcga aagccttgtc ctctttccag ctcccggcaa taccatccgc acttctccgc
      781 ttccgcggcg ccccggtaag gccagcgcct tcggccaaca cttcgatcgc cacctcgttc
      841 agactcttgc cggcaacacg cgcacgttct cgtaacgcag cgtcgagtgt ttcgggaatg
      901 cctcgtatgg tgtattgcat gcactcatta taggcagtgt aggcacagag tgcaagcacg
      961 tgatcgctac ggaatccgat cgaggtacct cgcctcttcg acgtcctcca ccgaacggat
     1021 ccgctgcagc gcggcctccg acattggcga gtcgacgtcg atttgcatga aggcgtcttt
     1081 gccgcgctgt tttcgcgagc acgacagtga cgcgatgttg attccctcct cggcgatgat
     1141 ctttgtcact tccgagatca tcccggggcg gtcgcggtag aagagcagga gcgttggcga
     1201 gtcacctttg aaacgagttt ggaagccgtt gatcttgaag acttcgatca cgccgccgcc
     1261 gatcgacgaa ccgctgatct cggcctcgcg cgttttgccg cgaatctcga tgcgcaccgt
     1321 gttcgggtga acgtcgccga ggtcttcttc ggagaaggtg atgtcgacgt ccatctgctc
     1381 ggcgatccga atcgcctcgg ggatgcgcgg atcatcgacg ctcaatccga tcgagccgcc
     1441 gacgagcgcg aagtcggagc cgtggccgcg gtacgtcgcg ccgagcggtg ggtgcaggta
     1501 aaagtgcacg gccaccggat cttcgtcgag gatctcgcgc gcgacgcggc cgaggcgcgc
     1561 ggtgccggcg gtgtgcgagc tggacggacc caccatcaca ggccccatca catcgaggat
     1621 cgagactttg cggtcgtgac gaatggccat ggagtttctg ttgcgcggcg ttgttgcgcg
     1681 ggatcagatc ccgtacactc cggcgtctgc atgcctgaaa agcctatcat cctcgtcgtc
     1741 gacgacgacc agccgattct cctgttgatg cggagtcttc tcaaggagtt cgggttcgaa
     1801 gcggtcacgg cagagaatgg caatcaggcg ttggccgccg cgcgggaacg ccgcccggcg
     1861 gtggttctgg tcgacaagaa catgccgggg atgacgggcg ccgaaaccat tcagagcctg
     1921 cgcgacgagc cgggcctcga gcggctcccg atcctcattc tcagcggcga gccggtgtcg
     1981 cgctccgaac tggcgggcct tcgcgcggac ggcgccgtgc tcaagccgtt cgatgtgaca
     2041 gcgctggtcg agcagatccg tgcgttcgtg gcggacggcg atggagctgg gcctcaaacg
     2101 tcccgcgcgt gagcacgcag ttttgctaaa gtccgcgacg gaggcgaccc gatgaccgac
     2161 atcaatgtcg accagctcgt tcacaccgct gtcttcggcg acggagatgc gaaggctgat
     2221 gcccgtagaa ccatccacat ggaagcgcgc cgccgcggcg cggtctcctc atcgatctat
     2281 ccgttctaca tgggcttcgg ccgcaacgag atcacgcgcc gcttcaccgt tcccgcgttc
     2341 aatatccgcg cgctgaccta tgacgccgcg cgcgcgctct ttcgcgtcgc catgcgccat
     2401 gacgccggcg cgttcatctt cgagatcgcg cgctcggaga tcggatacac cgaacagcgg
     2461 cctgccgagt acgccgcatg cgtgcttgct gccgcgatgg ccgaggggtt cgaaggaccg
     2521 gtgttcattc agggcgatca cttccaggcc agcgcgaaaa aatgggcgac gccggaaggg
     2581 caggcagcgg agatgaaggc gctcgaatcg ctcatcgacg aagcgctcgc cgcggagttt
     2641 tacaacatcg acatcgacac atccacgctc gtcaatctgg acttccccac gcgcgacgaa
     2701 cagcaacgcg cgaattacga ggggaccgcg catctgacga agtacatccg cgagcgggaa
     2761 ccgaaaggcg tgacggtttc ggtcggcggc gagatcggcg aggtcggcaa gtacaacacg
     2821 catccggagg aagtccgtgc ctacgccgac ggactgaagc gactcctcgg cggggtcacc
     2881 ggcattagca agatctccgt gcagaccggc acctcgcacg gcggtgtgcc gctccccgac
     2941 ggctcgatcg cgcaggcgaa gatcgacttc aacgtcctcc gcgaaacgac ccacatctgc
     3001 cgcgaggagt acggcatggc cggagccgtt caacatggtg cgtcgacgtt gccggagtcg
     3061 gtgttcaacc acttccccga atccgaagcg gtcgagatcc a
//
LOCUS       DATLVW010000609         4876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_522722, whole genome shotgun sequence.
ACCESSION   DATLVW010000609 DATLVW010000000
VERSION     DATLVW010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522722"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1511
                     /gene="hemL"
                     /locus_tag="VNN25_18070"
     CDS             222..1511
                     /gene="hemL"
                     /locus_tag="VNN25_18070"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXH93494.1"
                     /translation="MGKNSETLFSRAQEIIPGGVNSPVRAFKGVGGTPLFIDQALGSR
                     IFDADGLQYIDCVGSWGPMILGHGHRYVVEAVKKAATRGFSFGAPTVGEIELAEMIAQ
                     AIPSIEMVRLVNSGTEATMSALRLARAATGRKKIIKFDGGYHGHVDALLVKAGSGGAT
                     FNVPDSAGVPKEVTELTISVPYNNLEAVREAMDGEVAAVIVEPVAGNMGCVPPAEGFL
                     QGLRDICTENGALLIVDEVMTGFRVAYGGAQSLYNVKPDITTLGKIIGGGMPIGAYGG
                     SRELMQLVSPLGPMYQAGTLSGNPIAVAAGKATLGVLKNSSIYADLEERSGEFQTGVT
                     KSAEKHGVPLTVNRVGSMWTIFFTEGPVTDYESANKSNRERFSRFFHLMLGEGVYLPP
                     SQLEAAFFSAAHAKKDILQLIERVDRVLKKINWEFEK"
     gene            complement(1579..2349)
                     /locus_tag="VNN25_18075"
     CDS             complement(1579..2349)
                     /locus_tag="VNN25_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93495.1"
                     /translation="MILGLRNVVYAYPGANAVDNITATFDAKQLIALTGPNGSGKSTL
                     LKLVARVLTPYAGEIALDGKRLRDWHAKDYAKQVAYLPQDPDPAFSMRALDVVVSGRA
                     PFLGRFAWERDSDYDEAERALALCDAAHLGERYLDEMSGGERKRVFLARVLAARPQLI
                     LLDEPLSSLDVSHVQQFSALLRDIVDRTGVTVIYATHDLNWAAAYSDRMLVMQRGALA
                     LDAPPGEVMQPDAIRELFGFDAEAVRTGGRAWLVPRVG"
     gene            complement(2346..3329)
                     /locus_tag="VNN25_18080"
     CDS             complement(2346..3329)
                     /locus_tag="VNN25_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXH93496.1"
                     /translation="MSAVRHRSSLAGAFLLLGLLLVIALVLASSVGATNVSWRAALAG
                     DVTARSILVGIRLPRALLAALVGATLAVAGVTFQSLLRNPLADPFILGVSGGAACGAS
                     IATAAGLSRLPGVVPLIAFAGACGATGAVFLLARRREYTDPTRLLLSGLVLNAFFSAV
                     ILISFGLSRQSDLTAALRWMMGTLFGATWSDVALLAVLLVIATATLAAIGNDLRMLAF
                     GEDDAKSRGVNVERVKLIGFITASIVTGAAVSVSGIIGFVGLLVPHLVRMMWRRDFRF
                     LLPLCALGGGVLVVLADLLSRVVVTSSELPIGAFTAVLGVPFFLSLLRRNR"
     gene            3458..3787
                     /locus_tag="VNN25_18085"
     CDS             3458..3787
                     /locus_tag="VNN25_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93497.1"
                     /translation="MTPIEDGVIEAYDDCHQSGEIIQMIRLSIRVSDKHLERHLRAVA
                     RREGVSLSKAACILMRKGAGFAGQGESSPIGAALDRFIGSWTEEDEQEVLTSIGDFET
                     VDQGLWT"
     gene            complement(3998..4312)
                     /locus_tag="VNN25_18090"
     CDS             complement(3998..4312)
                     /locus_tag="VNN25_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HXH93498.1"
                     /translation="MSQRISKKLKPVTPGGLLREEFLVPMGISQYRLAKEIGVPAQRI
                     GEIVAGRRSITADTDLRLCRFFGLSNGYWLRAQAAHDTEVAEDVLAEALDRIKPWSGT
                     AA"
     gene            4371..>4876
                     /locus_tag="VNN25_18095"
     CDS             4371..>4876
                     /locus_tag="VNN25_18095"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HXH93499.1"
                     /translation="MNPVTKILAGALALVLFTAASPSNPKLTERQRALHVLNRLAFGP
                     RPGDVDAIAKSGVDAWIEQQLHPEAIPDRAVEARIALLPTMTLSNAQIVKTYYEPVLM
                     ARKKANAEAKDGIDDKKDIRRELIRDIPPNQRPQVVMNDLLSQRILRAAESDRQLNEV
                     MVDFWMNH"
BASE COUNT          965 a         1521 c         1571 g          819 t
ORIGIN      
        1 gggagtcggg agtcgggagt cgggagtcgg agaaaccgac cagtggatca gtgatctgta
       61 gatctggctc gatcagctct tggctatctc tgtcgctgcg tcggttgcct gtctgttgtc
      121 tcttgccgct gatctctcgt ctctgattcc ctgatctctc gtctctgatt gtctctgatc
      181 cctgatctct gatcctcccc ccatataatc cgccgcacta catgggaaaa aactccgaga
      241 ctctcttcag ccgcgcgcag gaaatcatcc ccggcggcgt caactctccg gtccgcgcat
      301 tcaaaggcgt gggcggaacc ccgctcttca tcgatcaggc cctcgggtcg cgcatcttcg
      361 acgccgacgg tctccagtac atcgactgcg tcggctcctg gggcccgatg atcctcgggc
      421 acggccaccg ctacgtggtc gaggcggtga agaaggctgc tacgcgcggc ttttcgttcg
      481 gcgcgccgac ggtcggtgaa atcgagctgg ccgagatgat cgcgcaggcc atcccgtcga
      541 tcgagatggt gcgcctcgtc aactccggaa ccgaagcgac gatgagcgcg ctgcggttgg
      601 cgcgcgcggc cacggggcgg aagaagatca tcaagttcga cggcggctat cacggccacg
      661 tcgacgccct cctcgtcaag gcggggagcg gcggcgcgac cttcaacgtc cccgattccg
      721 ccggcgtgcc gaaggaagtg accgagctga cgatcagcgt gccctacaac aatctcgagg
      781 ctgtgcgcga ggcgatggac ggcgaagtgg cagccgtgat cgtcgagccg gtagcgggga
      841 acatgggttg cgtcccgcca gccgaggggt ttttgcaagg gctgcgcgac atctgcaccg
      901 aaaacggcgc tttgcttatc gtcgatgaag tgatgactgg ttttcgcgtg gcctacggcg
      961 gcgcgcaatc gctttacaac gtgaagcccg acatcaccac gctcggcaaa atcatcggcg
     1021 gcggcatgcc gatcggtgcg tacggcggta gccgcgagct gatgcagcta gtctcgccgt
     1081 tgggaccgat gtatcaggca ggcacgcttt ccggcaatcc gatcgccgtg gccgccggca
     1141 aggccactct cggcgtgctg aagaattcat cgatctacgc cgatctcgag gagcgctcgg
     1201 gagagttcca gaccggcgtg acgaagagcg cggagaagca tggcgtgccg ctgacggtga
     1261 accgcgtcgg atcgatgtgg acgatcttct tcacagaagg tccggtcacc gattacgaaa
     1321 gcgcgaacaa atcaaatcgc gagaggttct cgcgcttctt ccatctcatg ctcggcgagg
     1381 gcgtttacct gccgccatcg cagctcgagg cagcgttctt ctccgcagcc cacgcgaaga
     1441 aagacatcct ccagctgatc gagcgcgtcg accgcgtgct gaagaagatc aactgggagt
     1501 ttgagaagta gggctcacct aatcgaccga tcggactgat cagactgatc caaccgatct
     1561 ctgatctccg atcactgctc accccacccg cggcaccagc catgctcgcc cgcccgttcg
     1621 caccgcctcg gcatcgaagc cgaagagctc gcggatggcg tctggttgca tcacctcgcc
     1681 gggtggggcg tcgagggcca gggcgccgcg ctgcatgacg agcatccggt cgctgtaggc
     1741 ggcggcccag ttcaagtcgt gcgttgcgta gatcacggtg acgccggtgc ggtcgacgat
     1801 gtcgcgcagg agcgccgaga attgctggac gtgcgagacg tcgagcgacg agagcggttc
     1861 gtcgagcagg atgagctgcg gccgggcggc gaggacgcgc gccaggaaga cgcgctttcg
     1921 ctcgccgccg ctcatctcgt cgaggtagcg ttcgccgagg tgggctgcgt cgcagagagc
     1981 gagggcgcgc tccgcctcgt cgtaatcgct gtcgcgctcc caggcgaagc ggccgaggaa
     2041 cggagcgcgt ccggagacga ccacgtcgag cgcgcgcatc gagaacgcgg gatctggatc
     2101 ctggggcaga tacgccacct gcttggcgta gtccttcgca tgccaatcgc gaaggcgttt
     2161 tccgtcgagg gcgatctcac ccgcgtacgg cgtgagaacg cgggccacga gcttgagcag
     2221 cgtcgatttg cccgagccgt tcggaccggt gagcgcaatg agttgcttcg cgtcgaaggt
     2281 cgcggtgatg ttgtcaaccg cgttcgctcc cgggtaggcg tagacgacgt tgcgtaagcc
     2341 gagaatcatc gattcctccg caggagcgag aggaaaaaag gcacgccgag tacggcggtg
     2401 aacgcgccga tggggagctc ggacgaggtg acgacgacgc gcgagaggag atcggccagt
     2461 accaccagca cgccgccgcc gagggcgcag agcggcagga ggaagcggaa gtcgcgccgc
     2521 cacatcatgc ggacgagatg cggtacgagc aggccaacga acccgatgat gccgctgacc
     2581 gacaccgcgg cgccggtgac gatcgacgct gtgatgaagc cgatcagttt cacccgctcc
     2641 acgttgacgc cgcgcgattt tgcgtcatcc tcgccgaatg ccagcatgcg caaatcgttg
     2701 ccgatcgccg cgagcgtggc cgtagcgatg acgagcagca ccgccagcaa ggccacgtcg
     2761 ctccacgtcg cgccgaagag cgttcccatc atccagcgca gcgccgcagt cagatcgctc
     2821 tgccgcgaca gaccgaacga gatgaggatg accgcggaaa agaacgcatt gagcacgagt
     2881 ccggagagaa gaaggcgcgt cgggtcggtg tactcgcgcc gtcgcgccag caggaagacc
     2941 gctccggttg cgccgcacgc acccgcgaag gcgatgagcg gcacgacgcc aggcagcctc
     3001 gacaatcccg cggcggtcgc gattgatgcg ccgcaagccg caccgccgga gacgccgagg
     3061 atgaagggat cggccagcgg attgcgaagc aaggactgaa acgtgacacc ggcaacagcg
     3121 agcgtcgccc ccacgagcgc cgcgagcagt gcacgcggaa ggcggatacc gacgaggatc
     3181 gagcgcgcgg tgacatcgcc cgcgagggcc gcgcgccacg atacgttcgt cgcaccaacc
     3241 gaagaagcga gcacgagcgc gatgacgagc agaaggccga gcagcaaaaa cgccccggca
     3301 agactcgatc gatgacgtac ggcgctcacg ctgcgcagtg tagtagcagg gaccgccggt
     3361 gcgaacgacg tggtcaagac gtagcgccgc cggcccggcg gctgtcccgc cggcggctcg
     3421 ccggcgactc tcaactcgca gggtctgacc ttgcgacgtg acgccgattg aggatggtgt
     3481 tatcgaggcg tacgatgact gccatcaatc aggagaaatc atccaaatga ttcggctatc
     3541 aattcgtgtc tccgacaagc atctcgaacg ccatctgcga gcggtggcgc gacgtgaagg
     3601 cgtctcgctg agtaaagccg cgtgcatcct catgagaaaa ggtgcaggat tcgccggcca
     3661 aggcgagtcg tcgccaatcg gcgctgctct cgaccgcttc atcggaagct ggaccgaaga
     3721 ggacgaacaa gaggtgctga ctagcatcgg cgactttgag actgtcgatc agggtctttg
     3781 gacgtgagtc atccgtttca aaccattgaa gttatcaggc cggatccaac caccgaggcg
     3841 ggacgccccg ctggccgcga gcgagacgct cgcactcctc agcgcaatcg aaaggagtgc
     3901 ggtcgtctcg accgcgggca ctcgggcgtc ctcgcccgag tgcagtcagg gcggaggtca
     3961 aactcgtcga cgatcttgct tacgtcgggt acggctatca cgccgccgtg cctgaccacg
     4021 gcttgatccg atcgagcgcc tcggcgagaa catcttccgc cacctccgtg tcatgagctg
     4081 cctgggcccg cagccagtag ccattggaca ggccgaagaa tcggcaaagg cggaggtcag
     4141 tatcggccgt gatgcttcgc ctgcccgcaa caatctcgcc gatgcgctgg gctggaacac
     4201 cgatctcctt ggcaaggcgg tactgagaga tgcccatcgg cacgagaaac tcttctcgaa
     4261 gcaatccacc aggcgtcaca ggtttgagct tcttcgagat tcgctggctc acaagtgtga
     4321 ggataagcca agtccgtaaa cacaccccca cctccgccgt ccaaccttcc atgaaccctg
     4381 tcaccaagat cctcgctggc gcactcgcgc tggtgctctt caccgcagca agcccttcca
     4441 atcccaaact taccgagcgc cagcgtgcgc tgcatgtcct caaccgcctt gctttcgggc
     4501 cgcggcccgg tgacgttgac gcgatcgcga aaagcggcgt cgatgcctgg atcgagcagc
     4561 agcttcatcc ggaggcgatt cctgatcgcg cggttgaagc gcgcattgcg ctgctgccga
     4621 cgatgacctt atccaacgcg cagatcgtga agacctacta cgagccggtt ttgatggcgc
     4681 ggaagaaggc gaatgccgag gcgaaagacg gtattgacga caagaaggac attcgccgcg
     4741 agttgatccg cgacatccct cccaatcagc ggccgcaggt cgtgatgaac gatctacttt
     4801 cgcagcggat ccttcgtgcc gccgagtcgg accggcagct caatgaggtg atggtcgact
     4861 tctggatgaa tcactt
//
LOCUS       DATLVW010000610         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_24765, whole genome shotgun sequence.
ACCESSION   DATLVW010000610 DATLVW010000000
VERSION     DATLVW010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24765"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VNN25_18100"
     CDS             complement(<1..407)
                     /locus_tag="VNN25_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93500.1"
                     /translation="MSGKNSLFAAALISLVVTARLAADCSPAVAVLVPPTACKSGTAT
                     VGILAVPGASYAWTVTGGQIIGDASGDRINIALGTGATATASVTMTSGTCVSKGSGVI
                     ALHDPFAVRVGAIQDGHASEPVIVSWVYDNGAPA"
     gene            complement(1053..2468)
                     /locus_tag="VNN25_18105"
     CDS             complement(1053..2468)
                     /locus_tag="VNN25_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93501.1"
                     /translation="MRKAIFALATLALAAQPVFATCGGGGGGGMGGTMPRSSMNSMGG
                     QTPPVNPYVVPWKSIGANDKPLTTPVVVMWFPAEAREVDNSELNVSRILTLYSAQCVG
                     MQLVKPDDANTIAKFDVVTKRPTVLLVVDGKVIDHVNPDGRFITSSSVENMIHHELFR
                     RESALDQLLETAKNESATNKDAAIADYQKVWEQHCLAPKQGREAQKALKHLGVIVKAE
                     DLRTSDPDLSAAMNARMTKAMTDALAAELAADYPKARALYRVAAAIDPADPVPSRFLG
                     ELYRHHTGEWDLARATFEHLLTIQPDPLSRAVALHGLGKMTIHMGDSAKGLALFEQSI
                     AAYPLALTYRNMAVYWNSERQRAKADVYVQKALALDPHDSYNLIFAATYLADSGRKEE
                     ALRIAKENEGDLAASYNLAAIYSLLGNKGKAMELLKRHFYQYERFDDVRAMEMWEARV
                     DYVFASMKDDPGFIELTKMAR"
BASE COUNT          497 a          802 c          872 g          572 t
ORIGIN      
        1 gccggcgcgc cgttgtcgta cacccaggag acgatcaccg gttcgctggc atgcccgtcc
       61 tgaatcgctc caacgcggac agcgaaggga tcatgcaagg caatgacgcc gctacccttc
      121 gacacacaag taccagacgt catcgtgacc gacgcagtgg ccgtcgcgcc cgtaccgagt
      181 gcgatgttga tccggtcgcc cgaggcgtcg ccgataatct gcccgccagt caccgtccac
      241 gcatagctcg ccccgggaac ggccaggatc cccaccgtgg ctgttcctga cttgcacgcg
      301 gtcggaggaa ccagcacggc cactgccggt gaacaatccg ccgcgagccg cgcggttacg
      361 acgagcgaaa tgagagcggc ggcaaagagg gagttttttc cggacattac ctcgcagagt
      421 acaggaagct ggagagggcg cttgtgaccc gcgctcgacc gcgaggtgat gggcgatacg
      481 ctggtcgtca acgcgtttcg tgggtgactc agaccgaacg tcgccctgat cccctccttg
      541 ctgacgcgaa atcccgagac gttcgtgcgc gtgtgccagc gatgtcaata acttactttg
      601 aatcagtacg ttacgcttga gtgggcataa ctcacgggcg gggaaaatac ctcggaggtc
      661 gaggtattta ccttcgaggc tgaggtattt accctcgagg gcgaggtatt tatcttcgag
      721 gccgaggtat ttgccttcga gggccaaggt atttacctcg gagggcgagg tatttacctt
      781 cggtattttg gatgaaagcg accgcttcac caatctttcc cttgccaaag agggaaggat
      841 tgacctgtcc gattgcaaaa atcgggttcg ctgagtccag accggctttc gagcgcgaaa
      901 accgcgtggg tgatggcacc gatcgcgatc ccgtcgctac accatcttga tcaggctcag
      961 cgcacccttc acgtccgtcg tcgaaagcgg tcggcgcacc acgcccgcgg acgccggctc
     1021 cgacccccga tcagcgactc ccgcggaaga aatcagcgcg ccatcttcgt cagctcgatg
     1081 aaccccgggt cgtctttcat cgacgcgaag acgtaatcca cgcgcgcttc ccacatttcc
     1141 atggcgcgga cgtcgtcgaa gcgctcgtat tggtagaagt ggcgcttgag cagctccatc
     1201 gctttgcctt tgttgccgag gagcgagtag atggcggcca ggttgtagga ggcggcgaga
     1261 tcgccttcgt tttctttggc gatgcggagg gcttcttctt tgcggccgct gtcggcgaga
     1321 taggttgcgg cgaagatgag gttgtaggaa tcgtgcgggt cgagggcgag ggccttctgg
     1381 acatacacat ccgcctttgc ccgctggcgt tcggagttcc agtacacggc catgttgcgg
     1441 taggtgagcg caagcggata ggcggcgatg gactgttcga agagggccag gccttttgcg
     1501 gaatcaccca tgtggatggt catcttgccg aggccgtgga gtgcgacggc gcgggagaga
     1561 ggatcgggct ggatcgtgag gaggtgctcg aaggttgcgc gcgcaaggtc ccactcgccg
     1621 gtgtgatggc gataaagctc gccgaggaag cgggagggaa cggggtcggc aggatcgatg
     1681 gccgcggcga cgcggtagag cgcgcgcgct ttcggatagt cggccgccag ctctgccgcg
     1741 agcgcgtcgg tcatcgcctt cgtcatgcgc gcattcattg ccgcggagag atcgggatcg
     1801 ctcgtgcgga gatcctcagc tttgacaatc acgccgaggt gtttgagtgc cttctgcgct
     1861 tcgcgtccct gcttcggcgc gaggcagtgc tgttcccaca ctttctgata atcggcgatg
     1921 gccgcatcct tgttcgtggc gctctcattc ttagcggtct cgagcagctg atccaacgcg
     1981 gactcgcgcc ggaacagctc gtgatggatc atgttctcga cgctgctcga tgtgatgaag
     2041 cgtccgtcgg gattgacgtg atcgatcacc ttgccgtcga cgacgagcag gacggtcggc
     2101 cgcttggtga cgacatcgaa cttggcgatg gtgttggcat cgtcgggctt gacgagctgc
     2161 atgccaacgc actgcgcgga gtagagcgtg aggatgcgcg agacgttcag ctccgagttg
     2221 tcgacttctc ttgcctcggc cgggaaccac atcaccacga ccggcgtggt cagcggcttg
     2281 tcattcgcgc cgatcgactt ccacggcacg acgtacggat tgaccggcgg cgtctgcccg
     2341 cccatcgaat tcatcgaaga ccgcggcatg gtccctccca tcccaccgcc gccaccgcca
     2401 ccgcaggtag cgaatacggg ctgggcggct agagcgagcg ttgcgagggc gaagatggct
     2461 ttgcgcatgg gtgtcgtcct tgattcgtgt gatggatcat taaagcgcag aaaaggcggc
     2521 cttcttccta ttgtcccggt tgttacaggg ttgtgatgtc ttgcctaagc aaagacaacc
     2581 tgttttcgtg agattgatca caccgctttc cactcatcgc gctatccttc ctgcattccg
     2641 tgaatcgtcc gactgctcca ttcgcgatct tctaccgctc cgggacgggt gaatcgtgct
     2701 tcgcctgtca cgcctgcggg atgcggcgcc acgatacgtt cgc
//
LOCUS       DATLVW010000611         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_109000, whole genome shotgun sequence.
ACCESSION   DATLVW010000611 DATLVW010000000
VERSION     DATLVW010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109000"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..312
                     /locus_tag="VNN25_18110"
     tRNA            225..312
                     /locus_tag="VNN25_18110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:259..261,aa:Leu,seq:taa)
     gene            334..1515
                     /locus_tag="VNN25_18115"
     CDS             334..1515
                     /locus_tag="VNN25_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXH93502.1"
                     /translation="MTTETVTPSAGAPLTILSEEEQMFQESVREFAIEKIRPLVHQMD
                     HDALMSKDLIKSFFELGIMGIEVPEQWGGAGSSFFNAVLVVEELSHVDASCGVLVDVQ
                     NTLVNNAIIRWGSEDQKSKYLPMLSSDTVGAYALSESGSGSDAFGLACKADDRGDHWV
                     LNGQKLWITNAFEANIFIVFANAKPDAGYKGITAFLIERDFPGFKVGKKEDKLGIRAS
                     STCELILEECQVPKENVVGEPGKGYKIAIETLNEGRIGIGAQMIGVARGALEHAIAYT
                     KERKQFGQSISEFQGVQFQIAEAATQLEAARLMVYNAARLKDAKRPFLREAAMAKLFS
                     SQVAEKITSLAVQLFGGNGYTKEYPVEKFWRDSKVGQIYEGTSNMQLSTIAKSIISGR
                     L"
     gene            complement(1496..1888)
                     /locus_tag="VNN25_18120"
     CDS             complement(1496..1888)
                     /locus_tag="VNN25_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93503.1"
                     /translation="MIWIMLPLLAFVFFGLYVRREAERIRAEDGTIVRGRVKNFAELP
                     HVVVRLKLAGSRMATLAELHVRQAIEDEIEQRGIGSVADAGSGKGWAHVSVVVADPAE
                     AAEKIRELLRERGLLDAAEVEASTDGPK"
     gene            complement(1885..2376)
                     /locus_tag="VNN25_18125"
     CDS             complement(1885..2376)
                     /locus_tag="VNN25_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93504.1"
                     /translation="MTETNTRPVELKEDRIPMAGIEETISNLIDLYGGELTASSANER
                     RFTLPLRRGLAAAGGVECTISWAPSDVGEAIVTLVCNRDVDAPKAQRIFLLVAGVVGS
                     LLFMIWPFFLHSRELGTLAWIGGVVAFAVYLMTLRKTSGGIAYDFLRRLSREQRSRQA
                     AHE"
     gene            complement(2427..2570)
                     /locus_tag="VNN25_18130"
     CDS             complement(2427..2570)
                     /locus_tag="VNN25_18130"
                     /inference="COORDINATES: protein motif:HMM:NF036547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH93505.1"
                     /translation="MSSVDRQLIERALETSGGRIREAARRLGLARNTLKSKIQKYKIT
                     AKD"
BASE COUNT          524 a          800 c          785 g          480 t
ORIGIN      
        1 atttcgatgg attgtcctag taaaactcag ggggttggct cgtaagggct ggtctccacc
       61 ccggaagggc gcccgttaaa cggcgccctt tttctttttg gcgtaagggg tcaggtcgaa
      121 accgaaaccg acggaaattg actggtcgta ggcgccaatt ccatcttccg tcggtttcgg
      181 tttcgacctg acctcttcca cacaacgtgt taccgttgtt ccgcgccccg gtggcgaaac
      241 tggtagacgc aagggactta aaatccctcg gtccgaaagg ccatgccggt tcgagtccgg
      301 cccggggcac caccgttccg atatgatccg cggatgacca ctgaaaccgt cactcccagt
      361 gcaggcgccc cgctgaccat cctctccgaa gaggagcaga tgtttcagga gagcgtgcgc
      421 gagttcgcca tcgaaaagat ccgcccgctg gtccatcaaa tggaccacga cgcgctgatg
      481 tcgaaggatc tgatcaagag cttcttcgag cttgggatca tgggaatcga ggtccccgag
      541 cagtggggcg gagctggctc gtcgttcttc aacgcggttc tcgtggtcga agagctttcc
      601 cacgtcgatg cctcctgcgg tgtgctggtc gacgttcaga acacgctcgt caacaacgcc
      661 attattcgtt gggggagcga agaccagaaa tcgaaatacc tgccgatgct ctccagcgac
      721 acggtcggcg cgtacgccct ctccgaatcc ggcagcgggt ccgatgcatt tgggctggct
      781 tgcaaggccg atgaccgcgg cgatcactgg gttctcaacg gacagaaact ctggatcacc
      841 aacgcattcg aggcgaacat cttcatcgtc ttcgcgaacg ccaagccgga cgccggctac
      901 aaggggatca ccgccttcct catcgagcgc gatttccccg gcttcaaggt cggcaagaaa
      961 gaggacaagc tcgggatcag ggcatcatca acctgcgagc tgatcctcga agagtgccag
     1021 gtcccgaaag agaacgtcgt cggcgagcct ggcaagggtt acaagatcgc catcgagacg
     1081 ctgaacgaag gacggatcgg gatcggcgcg cagatgatcg gcgtggcccg cggcgcgctc
     1141 gagcacgcga tcgcctacac gaaagagcgc aagcagttcg gacagtccat ctcggagttt
     1201 cagggagtgc agtttcagat cgccgaggcg gcaacgcaac tggaagcagc gcgcctgatg
     1261 gtctacaacg ccgcgcgact caaggatgcg aaacgtccgt tcctccgtga ggccgcgatg
     1321 gcgaaactct tctcctcgca ggtagcagag aaaatcacgt cgctcgccgt gcagctcttc
     1381 ggaggcaatg gctacacgaa ggagtatccg gtcgagaagt tctggcgcga ctccaaggtc
     1441 gggcagatct acgagggaac gtcgaacatg cagctttcga cgatcgctaa gtcgatcatt
     1501 tcgggccgtc tgtagacgct tcgacctcgg ctgcgtcgag cagaccgcgt tcgcgaagca
     1561 gctcccgtat cttctcggct gcttccgccg gatcggcgac cacgacgctt acgtgcgccc
     1621 accccttccc ggagccggcg tctgcgacgg atccgatgcc gcgctgctcg atctcgtcct
     1681 cgatcgcctg ccggacgtgg agctccgcga gcgtcgccat ccgcgagccc gcgagcttca
     1741 gccgcacgac gacgtgcggc aactcggcga agttcttcac gcgtccgcgc acgatggtgc
     1801 catcctccgc ccgaatgcgc tcagcttccc gacggacgta gagcccgaag aagacgaatg
     1861 ccagcagcgg caacatgatc cagatcattc gtgcgcggcc tgacgcgacc gctgctcgcg
     1921 agaaagacgc cgcagaaaat cgtaggcgat gcctccgctc gtttttcgga gcgtcatgag
     1981 gtagacggca aacgccacca cgccaccgat ccaggccagc gtccccagct cccgcgagtg
     2041 cagaaagaac ggccagatca tgaagaggag cgagccgacg acgcccgcga cgagcagaaa
     2101 gatcctctgc gctttcggcg catcgacgtc ccggttgcac acgagcgtga cgatggcctc
     2161 gcccacatcg gaaggcgccc aggagatcgt gcactcgacg ccgcccgcag ccgcaagtcc
     2221 gcgccgcagt ggaagcgtga agcggcgctc gttcgcgctg gaagcggtga gctcgccgcc
     2281 gtagagatcg atcagattgg agatcgtctc ttcgatccct gccatcggaa tgcggtcttc
     2341 tttgagctcc acaggacgtg tgttggtctc ggtcatcgtt ctccagagta gttcaagtgg
     2401 cagggagaat cctgaagcac ggagactcaa tccttcgctg tgatcttgta tttctggatc
     2461 ttcgacttca gcgtgtttct ggcgagaccg aggcggcgtg cggcctcgcg gattcggccg
     2521 ccggatgttt cgagggcacg ctcgatgagc tggcgatcga ccgacgacat cacctcgtcg
     2581 tagacctcg
//
LOCUS       DATLVW010000612         2883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_70606, whole genome shotgun sequence.
ACCESSION   DATLVW010000612 DATLVW010000000
VERSION     DATLVW010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70606"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="VNN25_18135"
     CDS             complement(<1..2104)
                     /locus_tag="VNN25_18135"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93506.1"
                     /translation="MAIQRDKIIASAEKLVAKGKIEPAIKEYERLLDDNPNDVNTLNR
                     IGDLWVRISRNDEAVKVFAKIADHYSKDGFFLKAIAIYKKINKLDPSKLDIYAKLADL
                     YAKQGLAMEAKSQYQVLADYYLKHGDPGNALVIYRKISELDPNSINVHVKLADLYSQN
                     NQKPEALKEYDRVGRMLLKRSMLDEAVQVFKKALKIDSSNVELVESLVTALLEAKDYE
                     NAIQIVQTSIETSGDDPRLLAIHGRILLAKGDVAGARAALERALSRDANAAGVREALA
                     DLYVRVGNADGALEMLAPLVEKAIGRGERGVAVETLNRILRVDSAHTPTLERLVALYA
                     RLNEETNILSSMSSLAEAHVAKGQYEQAAEVLDKLIQREPQNAQHRTKLQFVRSQMGG
                     VDTMPARPQASVPVAPLSMEIEEPVPSFDSFDDEPSLSLDLDSSEPIEINLDHGFATP
                     APARTPPAPAPPAPLPRLQPAVEQFAARVDQDDEGEDLDFITEHLTEADVFAKYGLAE
                     KATEHLRAIIERAPKHLVAHQKLYRVLLDEGEVDAARAAATQYIQVLQEKGDTAAIED
                     VGDEFAGRGYPLESAAPKPAVRAPVVEPSAPAPPAPAPPPEPAPVEEFADEMSFDIES
                     AVADFELSAPSTPEPAAPAFSFDSPDSSLSFEEPVSLLEPEPEPEPEPQPEPEPEPEP
                     ELEFTSPLSFESPIGEGFS"
     gene            complement(2085..2828)
                     /locus_tag="VNN25_18140"
     CDS             complement(2085..2828)
                     /locus_tag="VNN25_18140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXH93507.1"
                     /translation="MSAVKQRASKKSAKAKPKPGEKLVRNLFAELARVAEMSAMERRL
                     RDFGFRAIAGTDEVGRGCLAGPVVAACVILDAEPLLFGVNDSKLVDREDRVDICRLIL
                     RRARAVAVGVIEPRMIDAINILRASKLAMVEAVENLAVRPDVLLLDAVTIDVEMPQVP
                     LIGGDRRSVSIAAASIVAKVYRDLLMESYHDLYPVYDFRHNRGYATEGHQAALRLYGP
                     SPIHRRSFEGVDEPMLLFVRNGADGDPTR"
     gene            complement(2782..>2883)
                     /locus_tag="VNN25_18145"
     CDS             complement(2782..>2883)
                     /locus_tag="VNN25_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXH93508.1"
                     /translation="IEVERHGRVRRAKLYYLRDLRGKAARIEEKRKG"
BASE COUNT          510 a          888 c          916 g          569 t
ORIGIN      
        1 acgaaaagcc ttcgccaatg ggagactcga aggagagagg gctggtgaat tccagttccg
       61 gctcaggctc gggttccggt tcgggctgcg gttcgggctc cggttcgggc tccggctcaa
      121 gcaacgacac tggttcttcg aagctcagtg atgagtccgg cgaatcgaac gagaacgctg
      181 gcgccgcagg ctccggcgta ctcggggcac tcagttcaaa gtccgccacc gccgactcga
      241 tatcgaagct catctcatcg gcgaactcct ctaccggcgc gggttcgggt ggaggcgcgg
      301 gcgcaggcgg cgcaggcgcg gagggttcaa ccacaggggc tcgaaccgcg ggcttgggtg
      361 cggcagactc cagcgggtat ccgcgtcccg cgaattcgtc accgacgtct tcgatcgcgg
      421 cggtgtcacc tttctcctga agaacctgaa tgtactgagt cgctgctgcg cgcgccgcgt
      481 cgacctcgcc ttcatcgagc agcacgcgat acaacttctg atgcgcgact aggtgcttcg
      541 gcgcgcgctc gatgatggcc cgaaggtgct cggtggcctt ttcagcgagg ccgtacttcg
      601 caaagacatc cgcctcggtg aggtgctcgg tgatgaagtc gaggtcttcc ccttcgtcgt
      661 cctggtcgac tctggccgcg aactgctcga ctgcaggctg aagacgcgga agcggcgcag
      721 gcggtgcggg ggccggtggc gtacgcgcag gcgctggtgt cgcgaagccg tgatcgagat
      781 tgatctcgat cggctccgag gaatcgagat cgagcgagag agaaggttcg tcgtcgaagg
      841 aatcgaacga cgggaccggc tcctcgatct ccatcgacaa cggggcgacg ggaacgcttg
      901 cctgcggacg cgccggcatc gtgtcgacac cgcccatctg cgagcgaacg aattgaagct
      961 tcgtgcggtg ttgcgcgttc tgcggctcgc gctggatcag cttgtcgagc acctcggccg
     1021 cttgctcgta ctgcccctta gcgacatgcg cttcggcaag actgctcatc gacgaaagga
     1081 tgttggtctc ttcgttgagg cgcgcgtaga gggcgacgag ccgctccagc gtcggcgtgt
     1141 gcgcggaatc gacccgaagg attcgattca gcgtttcgac ggcgacaccc cgctctcccc
     1201 ggccgatcgc cttctccacg agaggcgcca gcatctccag tgcgccgtcg gcatttccga
     1261 cgcggacata aagatcggca agcgcctcgc gcaccccggc ggcattcgcg tcgcgcgaca
     1321 gcgcgcgctc gagcgcggcg cgtgccccgg cgacatcgcc cttcgcgagc agaatgcgcc
     1381 catggatcgc caggaggcgt ggatcgtcac ccgacgtctc gatcgacgtc tgcacgatct
     1441 ggatggcgtt ctcgtaatcc ttcgcctcga gtagtgccgt gaccagcgac tcgaccagct
     1501 cgacgttgct ggagtcgatc ttgagcgcct tcttgaatac ctgcactgcc tcgtcgagca
     1561 tgcttcgctt cagcagcatg cggccgacgc ggtcgtattc cttgagcgct tccggcttct
     1621 gattgttctg cgagtagagg tcggcgagct tgacgtgaac gttgatcgaa ttcggatcga
     1681 gctccgaaat cttgcggtaa atgacgagcg cgttgccggg atcgccgtgc ttcaggtagt
     1741 agtcagcgag aacctggtac tgactcttgg cctccatcgc caggccctgt ttggcgtaga
     1801 ggtcggcgag ctttgcgtaa atatcgagct tggagggatc gagcttgttg atcttcttgt
     1861 agatcgcgat cgctttgaga aagaagccgt ccttggagta gtgatcggcg atcttggcga
     1921 agaccttgac cgcctcatcg ttgcggctga tgcgcaccca gagatcgccg atgcgattca
     1981 gggtgttgac atcattgggg ttatcgtcga gaaggcgctc gtactccttg atcgccggct
     2041 cgatcttccc cttcgccacc agcttctcag cgctggcgat gattttatcg cgttggatcg
     2101 ccatcggctc cgttccgcac gaacagcaac atcggctcgt caaccccctc gaacgatctt
     2161 cggtgaatcg gcgaagggcc gtagagcctc agagccgctt gatgcccctc cgtcgcgtat
     2221 ccccgattgt gtcgaaaatc gtaaacaggg tagaggtcat gatacgactc catcaataga
     2281 tcgcgataga ccttcgcaac gatcgatgcg gcagcgatag agacgctgcg ccggtcgccg
     2341 ccgatcaacg gaacttgagg catctccaca tcgatcgtca cggcgtcgag cagaagtacg
     2401 tcggggcgca ccgcgaggtt ctcgaccgcc tcgaccatcg ccagcttgct ggcgcggaga
     2461 atgttgatcg catcgatcat ccgcggctcg atcacgccga ctgcgacggc gcgggcccgg
     2521 cggagaatca accggcagat gtcgacgcgg tcttcgcggt ccacgagctt ggaatcgttc
     2581 acgccgaaga gcagcggctc cgcgtccagg atgacgcagg cggcgacgac cggaccggcc
     2641 agacagccgc gtccgacttc gtcggttccg gcgatggccc gaaaaccaaa atcgcggagc
     2701 cggcgctcca tcgccgacat ctcagcgaca cgcgccaact ccgcgaagag gttccggacg
     2761 agcttttcgc ccggcttcgg tttagccttt gcgcttttct tcgatgcgcg ctgctttacc
     2821 gcggagatcc cggaggtagt agagctttgc gcggcggacc cggccatggc gttcaacctc
     2881 gat
//
LOCUS       DATLVW010000613         2371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_522845, whole genome shotgun sequence.
ACCESSION   DATLVW010000613 DATLVW010000000
VERSION     DATLVW010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522845"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..571)
                     /locus_tag="VNN25_18150"
     CDS             complement(23..571)
                     /locus_tag="VNN25_18150"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXH93509.1"
                     /translation="MTAFICLLRGVNVGGNKMLKMESLKKLFSSLGLEDVSTYLQSGN
                     VVFRSDVTDRAVLVRKIEEAIRKATALDVRIILRTAAEMKKVIAANPLDPAERNPKAL
                     LVVFLNGDLGKGAKELLSGLKIASEEIHFGNRELYLYFPEGIAGSKLSNALTEKKVGV
                     TVTARNWNTVNALMAMGEEIGG"
     gene            639..929
                     /locus_tag="VNN25_18155"
     CDS             639..929
                     /locus_tag="VNN25_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93510.1"
                     /translation="MRRLGFAFALATTLALAASTTPSPKPAAPVDFEKQIQPILEAKC
                     QPCHFPGGKMYAKRPFDRPATIVDLGEKLFTRIKDEDQRKLIRAFLAEQKAR"
     gene            981..>2371
                     /locus_tag="VNN25_18160"
     CDS             981..>2371
                     /locus_tag="VNN25_18160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH93511.1"
                     /translation="MAFACIRCNTEIDRDFKACPHCGEPITDFLREYADKPIDGKYRI
                     IQRLGIGGMGEVFKVEHTFLGSVRVVKIIRAQISGSTEANERFLREARLATKVQHPNV
                     ATLYDFSALPDGSHYMVWEFIEGENLAQLIRRSGTLSPRHAVRIAIQALAGLDAIHRA
                     GIVHRDISPENLMITHTADGSEFVKIIDLGVAKATESDVAMTQTGMFVGKFRYASPDH
                     LGFLGAGEHIDGRADLYSLAIVLFEMVTGRPPFEATSPHAYILHHSRDEYLNSPDLDR
                     ITGSPALQTVLTRALDRDRNKRFTTAREFADALIAVERDLPDEAGKTVTGTFDPDATM
                     RTERTRNGRTAAAAVIATDIAPPARTVPAPAPTSAPAAAAPGAEQGPTVITAAGVAPA
                     TIASSVPSSYSPPPPPPSAPAPPTIAATMPGAAPQRSSTGLIIAITFIALLLFGALAA
                     LAVWRFWPRSTVTT"
BASE COUNT          444 a          843 c          663 g          421 t
ORIGIN      
        1 gcggctgatc aaggaaccct gatcaccccc caatctcctc ccccatcgcc atcagggcat
       61 tcacagtgtt ccagttcctc gcggtcactg tcacgcctac ctttttctcc gtcagcgcgt
      121 tcgagagttt ggagccggcg atgccctcgg ggaaatacag gtagagttcc cggttgccga
      181 agtggatctc ctccgaggcg attttcagac ccgacaacaa ctcctttgct cccttgccga
      241 ggtcaccgtt caggaagacg acgaggagag cctttggatt ccgttcggct ggatcgagcg
      301 gattcgccgc aatgactttc ttcatttcgg ctgcggtacg aaggatgatg cgaacgtcga
      361 gggcggtggc ctttcggatc gcctcctcga tcttccggac gagcaccgcg cgatcggtca
      421 cgtcgctgcg gaacaccaca ttgccgctct gaaggtacgt gctcacgtcc tccaacccga
      481 gcgagctgaa tagcttcttc agcgactcca tcttgagcat tttgttgccg ccgacattga
      541 ccccgcgcag aagacagatg aatgcagtca tgagtcaaag agtagcagtt caccaagccg
      601 ttcgtgacgt cggtgcgcga agagcctatc ctgtccggat gcgacgtctc ggcttcgctt
      661 tcgcgcttgc cacgaccctg gcgctggctg cgtcgacgac gccctcgccc aagcccgccg
      721 ctcccgtcga cttcgaaaag cagatccagc cgatcctcga ggccaagtgc cagccttgcc
      781 acttccccgg tggcaagatg tacgcgaagc gccccttcga ccgccccgcg accatcgtcg
      841 acctcggcga gaaactcttc acccggatca aagacgagga tcagcgcaag ctcatccgcg
      901 cattcctcgc cgagcaaaag gcgcgatagc ttcgccgggc ggcacagccc cgcgcatgac
      961 gcgttatcat tccgtcgctc atggccttcg cttgcatccg ttgcaacacc gagattgacc
     1021 gcgacttcaa agcctgtccg cactgcggcg agccgatcac cgatttcctc cgcgagtacg
     1081 ccgacaagcc gatcgacggc aagtaccgca tcatccagcg cctcggcatc ggcgggatgg
     1141 gcgaagtctt caaggtcgag cacacctttc tcggctcggt ccgcgtcgta aagatcatcc
     1201 gcgcgcagat ctccggcagc accgaggcga atgaacggtt cttgcgggaa gcgcggctgg
     1261 ccaccaaggt ccagcatccc aacgtcgcca cgctgtacga tttctccgcg ctccccgacg
     1321 gctcgcacta catggtgtgg gagttcatcg aaggcgagaa cctcgcgcag ttgatccgca
     1381 gaagcggaac gctgtcgccg aggcatgcgg tgaggatcgc gattcaggcg ctggcgggac
     1441 tcgatgccat tcaccgcgcc ggcatcgtcc atcgcgacat cagccccgag aacttgatga
     1501 tcacgcacac agccgacggc tccgagttcg tcaagatcat cgacctcggc gtggccaagg
     1561 cgaccgaatc tgatgtggcg atgacgcaga ccggcatgtt cgtcggcaag ttccgttatg
     1621 cctcccccga tcacctcggc ttcctcggcg ccggcgagca catcgacggc cgtgccgatc
     1681 tctattcgct ggccatcgtt ctcttcgaaa tggtcaccgg gcggccaccg ttcgaagcca
     1741 cgtcgccgca cgcatacatc ctccatcact cgcgcgatga gtacctgaac tcgcccgatc
     1801 tcgaccgcat cacgggcagt ccggcgttgc aaaccgtcct cacgcgcgcg ctcgatcgcg
     1861 accgcaacaa gcgcttcacc accgcgcgcg agttcgccga tgcgctgatc gccgtcgagc
     1921 gcgatctccc cgacgaagcg ggaaagacgg tcaccggaac attcgacccc gacgctacga
     1981 tgcgcacgga acgcacccgc aacggacgga cagccgcagc agccgtgatc gcaaccgata
     2041 ttgcgccacc ggcgcggacc gttccggcgc cagccccgac atcggctcct gcggcggccg
     2101 ccccgggagc cgaacaaggg ccaaccgtca taaccgcagc cggcgtcgct cccgccacga
     2161 ttgcatcgag cgtcccctca agttactcgc caccgccgcc gccgcccagc gcgcccgctc
     2221 cgccgacgat cgccgcgacg atgcccggtg ccgcaccgca gcgaagcagc acgggactca
     2281 tcatcgcgat caccttcatc gctcttctcc ttttcggagc gctggccgcc ctggcagtgt
     2341 ggcggttctg gccgcgatca accgtcacga c
//
LOCUS       DATLVW010000614         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_396431, whole genome shotgun sequence.
ACCESSION   DATLVW010000614 DATLVW010000000
VERSION     DATLVW010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396431"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..760
                     /locus_tag="VNN25_18165"
     CDS             299..760
                     /locus_tag="VNN25_18165"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXH93512.1"
                     /translation="MESMVPVPRRRFINWLLGGTFGALVASVIYPIIRFASPPRIPEA
                     TTNQVEAGDVSDPVFSAKGFKIIRFGAEPVILLKASETEFRAFSATCTHLDCIVGYQK
                     DRSQIFCNCHGGTYDLNGRNVAGPPPRPLIAYKVNLIAKGSEPAKIVVSRA"
     gene            769..1929
                     /locus_tag="VNN25_18170"
     CDS             769..1929
                     /locus_tag="VNN25_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXH93513.1"
                     /translation="MLERTKSWLDDRLGLSKIIEFGSHKTVPAHRYSVFYYLGGMTLF
                     FFLVQVCTGVLLMLYYRPSANEAFESVEFIMTVVPFGWLIRSIHSWSANLMVFFAVLH
                     MVSVFFLKAYRGPRELTWMSGVVLLFLIMGFGFSGYLLPWNQLAFFATKVGTDISGVV
                     PIVGPWMVRFLRGGDRVSGGTLSRFYGWHVALLPAITFLILVVHLALVQVKGMSVPAS
                     CEDEAKQRKPMKFFPHFAMRDLSGWIFALGVLAALAALFPWELGEKADLFAPAYENIR
                     PEWYFVFMFQTLKLIPGGEIAGIEYEAIPIMFFGAAGLLLLLIPFLDRGAQRTGRSKA
                     WSIGGVAVLLWALGMTCWGYASLLPLYVVLISALLLGLLAFITRGSRKPEDR"
     gene            1926..2384
                     /locus_tag="VNN25_18175"
     CDS             1926..2384
                     /locus_tag="VNN25_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93514.1"
                     /translation="MRIAAVLFASATLLVMSSAAAAPPAGSTAASTTATTTAAPARVP
                     APVPSAAPKPETSCIHCHGGELFDEAGRAKVKHFGVDVHSQVGLSCHDCHGGNPDPKL
                     GDDIAGAMDPKYAPNPYVGAPKRTAIPDFCGRCHSSADYMKRFNPAARVM"
BASE COUNT          416 a          814 c          683 g          484 t
ORIGIN      
        1 gagcagaacg gtgccagaac cactgtccgg ctcaggcgca acggccaccg tcgccggaag
       61 cggcgccttc gccaaaggcc ggccactctc caaaagtgcc agtcgttctt cgatgaaacg
      121 cagccgctga tcgatcccct cgagcttttc gtcaacgttc atacgtcacc caagcatgtt
      181 caggactatg ctgaccgtgt cacgccgcac cggtgagagt gacctggaac actataggcc
      241 ccatgtgacg cgcggtttga tacacccgtt gcatcacatg agaaggagcg aatcgtcgat
      301 ggaaagtatg gtcccggtgc cgcggcggcg ctttatcaac tggctcctcg gcggcacatt
      361 cggcgcgctc gtggcgagcg tcatttatcc catcatccgc ttcgccagcc ctccgcgaat
      421 tcccgaagcg acgacgaatc aggtggaggc aggcgatgtc tccgatccag tcttctcggc
      481 gaagggattc aaaatcatcc gcttcggcgc cgagcctgtg atcctgctca aagcaagcga
      541 gaccgagttc cgcgcgttct ccgccacatg cacgcatctc gactgcatcg tcggatacca
      601 gaaagaccgc agccagatct tctgcaactg ccacggcggc acgtatgacc tcaatggacg
      661 aaacgtcgcc ggcccgccgc cgcgcccgct catcgcctac aaagtgaacc tcatcgccaa
      721 aggctccgag ccggcgaaga tcgtcgtctc gcgcgcttag gctgaaccat gctcgaacga
      781 acaaaaagct ggctcgacga ccgtctcggt ctgtcgaaga tcatcgaatt cggctcgcac
      841 aagaccgtcc ccgcccaccg ctacagcgtc ttctactacc tcggcgggat gacgctcttc
      901 ttcttcctcg tgcaggtctg caccggcgtc ctcttgatgc tctactaccg gccgtcggcg
      961 aacgaagcct tcgagtcggt cgagttcatc atgaccgtcg tcccgttcgg ctggctgatc
     1021 cgctcgatcc actcctggtc ggcgaacctc atggtcttct tcgccgtgct gcacatggtc
     1081 agcgtgttct ttctgaaagc ctaccgtggg ccgcgcgagc tgacatggat gagcggcgtc
     1141 gtgctgctct tcctgattat gggattcggc ttctccggct acctgctgcc gtggaatcaa
     1201 ctagcgttct tcgcgacaaa ggtcggcacg gacatctccg gcgtcgtacc gatcgtcggc
     1261 ccctggatgg ttcggttcct tcgcggcggc gaccgcgtat ccggcggcac gctgtcacgc
     1321 ttctacggct ggcacgtcgc cctccttcct gcgatcacgt ttctcatcct cgtcgttcac
     1381 ctggcgctcg tgcaagtgaa aggcatgagc gttccggcgt cgtgcgaaga cgaagcgaag
     1441 cagcgcaagc cgatgaagtt cttcccgcac ttcgccatgc gcgatctgtc gggatggatc
     1501 ttcgccctcg gcgttctggc cgcgctcgca gcgctctttc cgtgggagct cggcgagaag
     1561 gccgacctct tcgctcccgc gtacgagaac atccgtccgg agtggtactt cgtcttcatg
     1621 ttccagacat tgaagttgat cccagggggc gagatcgccg gcatcgagta cgaagcgatc
     1681 ccgatcatgt tcttcggcgc cgcgggactg cttctgcttc tgatcccatt cctcgaccgc
     1741 ggcgcgcaac gcaccgggcg cagcaaagcc tggtcgatcg gcggcgtggc cgtgcttctg
     1801 tgggccctcg gcatgacgtg ctggggctac gcctccctgc ttccgctcta cgtcgtcctg
     1861 atcagtgcac tactactcgg gcttctggcc ttcatcacac gcggcagccg caagccggag
     1921 gatcgatgag aatcgctgct gtgctcttcg cctcggcgac gctgctcgtc atgagcagtg
     1981 cggccgctgc gccgcctgcc ggctcaacgg ctgcttcaac taccgccacc actaccgctg
     2041 cgcccgctcg cgttccggcg ccagtacctt ccgcagcacc gaagccggaa acgtcgtgca
     2101 ttcactgcca cggcggcgag cttttcgatg aggctggacg cgcgaaggtc aaacacttcg
     2161 gcgtcgacgt tcactcgcag gtcggactct cctgtcatga ctgccacggc ggcaatcccg
     2221 atccgaagct cggcgacgac atcgccggcg cgatggatcc gaagtacgcg ccgaatccgt
     2281 acgtcggcgc gccgaaacgc accgccatcc ccgatttctg cggccgctgc cattcctccg
     2341 ccgactacat gaagcgcttc aatccggcgg cgcgcgtcat gtagtcggcg gaggaat
//
LOCUS       DATLVW010000615        10688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_195441, whole genome shotgun sequence.
ACCESSION   DATLVW010000615 DATLVW010000000
VERSION     DATLVW010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195441"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VNN25_18180"
     CDS             <1..1660
                     /locus_tag="VNN25_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93515.1"
                     /translation="ASGEPWLANPQTGVFYPPAWIFVILPFTAAYTLYLLIHVVLLGC
                     GGYLLFARLTRPGGAALAAALTLMFCGPVMSLLDVSNNLTTFAWLPLILWCAFSGVSA
                     RASAVAIAMSFLAGEPFFAAVAALLFAVVRRRGARDLIDVALTSFALSSIQLMPFLSM
                     VAGSDRANGGVPRATMFSDSMPLADWLRIVIPPNLGGNPFDPRLGQHFILIVYVGTLT
                     VALALVGVIAGRKRAIGWSALIVICLLVGMGDRLTPVAELLARLPVTLFRYPARVVPL
                     AVLAICALAAIGCDRVIPGRWQFAIAVVMFADVVLQIQPLLITTKFNLHRVPYATSIG
                     RNAKILRIDQPAVFDRDGWISGYLNLYDRRFDASTPAPLFSQRYAAYLQSMQTRHDRA
                     AIDALSVGYILAPRALAGLQPVAAHEGIVVHRNPAAFPLAFCRDDVTQRIAGVKSLAF
                     TPSSVFIDVDMPADGEVILTQQTAPGWHVTVDGDAATPLENSLFRGVHVARGSHAVKW
                     SYRPLPLLIGAILSLAALVRLFLSNWFVKRGGRINFLRTSRKNA"
     gene            1745..2032
                     /locus_tag="VNN25_18185"
     CDS             1745..2032
                     /locus_tag="VNN25_18185"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH93516.1"
                     /translation="MNQIIRELIGSGITLEQAVEAFEGKYIVAAMSETRGNVTAASKA
                     LGVHRNTLHNKLRSQTMLNGFADAIRPVRRRISANRVTAPKSSNGKTTRSR"
     gene            2046..4301
                     /gene="rnr"
                     /locus_tag="VNN25_18190"
     CDS             2046..4301
                     /gene="rnr"
                     /locus_tag="VNN25_18190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXH93517.1"
                     /translation="MPQSLSLPGSTGLKQSITDLLRKRGSRLLPVKEIHERLEGIEAS
                     REEVERAVEELEQDGIIVAVRGKRYSLLEFTPYHAGRIRVHPDGYGTVFGGGEEPDIF
                     IDRRSMKGAMNGDLVVVRVDKHSPKFHTIRQRKLIIGEVTTVLRRAHRTVVGRFHADP
                     EQPFVTPFDSRLDHDIVIDRNGTMDARDEEMVNVEIDRYPDRTSHIARGRVVEVLGFI
                     GEPGVDIELVIRKLQIPHRFPPEVLAAADAIPDEVTAAEIAKRVDLREHNIVTIDGET
                     AKDFDDAVEVQLLPNGNYLLGVHIADVSHYVTEGSALDEEAFERGTSVYFPGRAVPML
                     PERLSNGICSLNPRVERLTFSVDIEIDRKGRFVDRKVYRSVIRTKERTTYTNVNAFLV
                     NEGVAQPSGMPPHGVIVPPPEELRARYGYLLDDFTRMHALYDILRKRREARGSIDFDL
                     PEAEVILDSEGVIDAIKPTGRNVAHRIIEEFMLAANEVVASELVFANQPGIFRVHQQP
                     DPQKLEDLREILKEFKLTLRGDLEEMRPGELQRVMKSVEGTPEERFLSNIVLRSMKRA
                     YYSEENVGHFALAIANYAHFTSPIRRYPDLIVHRRLGEMVDKGPLYGERLAEVERAHP
                     LYAAQSSDRERRADEASREVLEWKKVIFMRDKVGLKFTGIVTGVAAFGLFIELDEIFV
                     QGMVPVATIGGDFWVFREREHRLRGDSTGREMRLGDKVTIEVKSIDEDRHQLEFRLIE
                     IGGSPIALRER"
     gene            4337..5425
                     /locus_tag="VNN25_18195"
     CDS             4337..5425
                     /locus_tag="VNN25_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93518.1"
                     /translation="MADQNDLATQIRNLVDQRVALETKLQNEPGELSESNARKYDAAY
                     NDLQRKLAAIFKQTDEEQPRITLMLELMRILENRLVYLHNFIVDATSDNRVFAQLVME
                     FIAQLLAQRSNVLTNLEATYYGAVATLYAGDLAGARKGFAAACASEESDEANDIKYKS
                     YVILGHLSHEERDYAKAKDLHDRSMRYSQNNNVTAQALALKALNSYALGDRADALHLF
                     QESLELFDANQPFFNSYFFRNALLFCGAIHFDGKDYASAEPYYRRVLANVEQTSYDYF
                     DAQTHLGRIAYTQQHYDDAVACFEKAVQAQKLSENESIVDTWFWIARTHLKRNDSAAA
                     RTYLEKIAASDVKYEKKAQAAELLQKIA"
     gene            5689..7104
                     /locus_tag="VNN25_18200"
     CDS             5689..7104
                     /locus_tag="VNN25_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93519.1"
                     /translation="MKTVLVAISVCLLSVLNATAQWIDCTNAPAGSKTCTQTSPVGIG
                     TLSTQYQLSVLGTQNTDGIQINSGNATMLKLDTNRTDGLNYAFGLNRLAAGDFALYEG
                     TSPRGDSFGGTRRLYINSVGNVSLQPDSGNVGIGTTSPSVRLSLGGGPGGIKQLLYDE
                     PGFSPDYKMGFGVNLANPGVSSSIDVLMGFGTGADNSFNVVKPTTGWPYYTSTYTNST
                     FTKLLTVLENGNVGIGTFSPESIQSGGPALHLYGAGPSLRIQDTASNGDWNLFARTGA
                     STNLFRIYDNAHSADRLTIDASGIVNVGVALTVGPAGLIHGNSLNVNGDVTVSGNLAA
                     RFQDIAEWVPVTESMTAGTVVVVSNDASNTVAPSLHAYDTGVAGVISAQPGLTLGEGS
                     TTKAKVATTGRVRVRVDATTHPIARGDLLVTSDKPGTAMFSEALDLGGVKIHRPGTLI
                     GKALEPLAKGQGEILVLLSLQ"
     gene            7118..7768
                     /locus_tag="VNN25_18205"
     CDS             7118..7768
                     /locus_tag="VNN25_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93520.1"
                     /translation="MKISARLLTVLLVLSIPVTGLVAAPVVPAITFDSTHVIATGFHS
                     ARDIVIFGVGTGPGPYFLRLMRFTDTLTADSAGTVRYEIANGVPERSVWFAVDTQTPD
                     YAVGSPQPALLHATLSAPGIRRGTQPAADTVDLDRRLVDLLLVRPGSGAWAGSCGRNS
                     LKDRNRGKSGAMQFSAADIDAAPKTIGRPSTILPSDLIVIIDSETLEYFIGSPRQL"
     gene            7779..>10688
                     /locus_tag="VNN25_18210"
     CDS             7779..>10688
                     /locus_tag="VNN25_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93521.1"
                     /translation="MRNRLLFALSLSIVIALSLHAQTHPNIEKGFAADKMYQFGDIDH
                     VNLFNGNLSLTIPIGSTPVSDRLSLSLTLVYNTKLWTALDKEYNVGGQTGRAPLYTPE
                     RRSNAGLGWTLTLGRLLDPSETDVNSIINGATRFAYMSPDGSDRLFYDTLHDEETATA
                     GVLYSRDNTYLRLSDGGAGKILESPDGTIREFESYIDSITNTTCPTPPCGSYTRWRLT
                     TIRDRFGNHIDIDYSTAGQWRITDHYGRLTVVHFAAHASDVDAEYLQQVSSIDVPTTG
                     GGTASYGFSYEGVDDPGNPQDKWVKTGACAVPHPDTPVGYRLPLLKSLSLPDSNVYTF
                     TYTGPIGDTLDSVAPGGNEACTAGGLASVTLPTKAKISYEYDVYDLPTLACHDDPDPI
                     AESSGIKTRTVQDPFNASNDQAWHYTVAGEQSFTYLGGQMTCPGPIQVDAGPSQYINT
                     VTVSTSAEPVAQKTRYYFSVYHGKLSDANGTTVHDYGLPFSRIHPFDTTKTRFLSTEV
                     LDASDTPKRSTYLSYIYEPAGSAVTSSATYAFNPRITGSRTVFLDDEKTPGNPYYNDT
                     AFSEFDGVGHYRTKTVSGNMDGSTRIITTSYNTVDPLVNPGALETGCFESCAGGVAFT
                     PPLTSAAWVLNLYPTSTTSESGAQGSPSVQEACFDKTTGFLNGTRTRAGASRDSKDLI
                     TVFGSSSDFSLTGNLHGAVTSEKYYGGDVNPLTSSSSQTLCDTVTSSTAPGTLGYDIR
                     HTYVNAMPAISQYYTNGASVGFKFLDQDTDYSTSLVVHSRDTAGVQTAYTYDSSRRVS
                     SVQAGGDPIINYTYTNATSSAGANVTIDQTSTTSGAPKASFDYDGLGRLVLQSRTLPR
                     ASLGDATRWTRQKTVYDAMGRKTQVSEWEESSSPSHFTVSSDFDRFGRPGTITRPDSS
                     TTTFAYQGGRRIDRTAHIHTQSGADTNATTTEVYDLHGRLYKVNEPANNTIATYSY"
BASE COUNT         2156 a         3394 c         3059 g         2079 t
ORIGIN      
        1 tgcgtcggga gagccatggc tcgccaatcc gcagaccggc gtcttttatc cgcccgcctg
       61 gatcttcgtc atccttccgt tcaccgcggc gtacacgctc tatctcctca tccacgtagt
      121 gctcctcgga tgcggcgggt atctgctgtt cgcgcggctg acgaggccgg gcggagcggc
      181 gctcgcggcg gcgctgacgc tcatgttctg cggtccggtg atgtcgctgc tcgatgtatc
      241 gaacaatctg acgacgttcg cctggcttcc gctgatccta tggtgcgcat tctccggcgt
      301 ctccgcgcgc gccagcgcgg tggctatcgc catgtcgttt ctagcagggg agcctttctt
      361 cgccgcggtg gccgcgttgc tatttgcagt cgtgcggcgg cgtggcgcgc gcgacctcat
      421 cgacgtcgcg ctgacgtcgt tcgctctgtc gtcgattcag ctcatgccgt tcctttcgat
      481 ggttgccggc agcgaccgcg caaacggcgg cgtgccgcgg gcgacgatgt tcagcgactc
      541 gatgccgctc gccgactggc tccgcatcgt catccctcca aacctcggcg gcaacccatt
      601 cgacccgcgc ctcggacagc atttcatcct cattgtttat gttgggacgc tgaccgtcgc
      661 ccttgcgcta gtcggtgtga tcgccggccg caagcgcgca atcggatgga gcgcgctcat
      721 cgttatctgt ctgctggtcg ggatgggcga tcgtctgacg ccggtcgcgg agttgctcgc
      781 ccgcttgccc gtcacgttgt tccggtatcc ggctcgcgtc gtgcctctgg cggttctggc
      841 gatctgcgcg ctggccgcga tcggctgcga ccgcgtgatc ccgggacgct ggcagtttgc
      901 gattgctgtc gtgatgttcg ccgacgtcgt gctgcagatc cagccgctgc tgatcacgac
      961 gaagttcaac ctgcatcggg ttccgtacgc cacgtcgatc ggccgcaacg cgaagattct
     1021 gcgcatcgac caaccggccg tcttcgatcg cgacgggtgg atttcgggct acctcaatct
     1081 ttacgaccgg agattcgacg catcgacgcc ggcgccgctc ttctcgcagc gttacgccgc
     1141 gtacctccag tccatgcaga cgcgtcacga ccgagcagcg atcgatgcgc tctccgtcgg
     1201 ttacatcctg gcgccgcggg ctctggccgg gcttcagccg gtggcggcgc acgagggaat
     1261 cgtcgtccac cgcaaccccg ccgcctttcc gctcgcgttc tgccgcgacg acgtcacgca
     1321 gcgcatcgcg ggcgtcaagt cgctggcatt cacgccatct tcggttttca tcgacgtcga
     1381 catgcccgcg gatggcgagg taatccttac tcagcagact gcgccgggat ggcacgtcac
     1441 ggtggatggt gacgccgcaa cgccgctgga aaactcactc tttcgtggag tgcatgtggc
     1501 acgcggatcg cacgcggtga aatggagcta tcgtccgctt ccgctcttga tcggagcgat
     1561 tctttcgctc gctgcgctgg tgcgcttgtt cctttcgaac tggtttgtca agcgtggcgg
     1621 gcgtataaat tttcttcgca catcgcgtaa aaatgcgtag caattttcat gaagtagggg
     1681 tgtagaatcg cccccttcca aacgaccgtc aggggcatga tcaatggtta gtatcgatga
     1741 tcgcttgaat cagatcattc gcgaattaat tggcagtgga atcactctcg agcaggccgt
     1801 ggaggctttc gaggggaaat acatcgtggc cgcgatgtcg gaaacgcgcg ggaacgtgac
     1861 cgcagcgtcg aaggctctcg gcgtccaccg caacacgctg cacaacaagc tccgctcgca
     1921 gacgatgctc aacggatttg ccgacgccat ccgccccgtg cggcgccgca tctccgcgaa
     1981 tcgcgtgact gcgccgaagt cttcgaacgg caaaacgacg cgctcgcgtt gacgcgggga
     2041 gcctcttgcc tcagtcgctt tcgctccccg gatctaccgg actcaaacaa tcgatcaccg
     2101 atcttctccg caagcgcggc agccgtctcc ttcccgtcaa agagattcac gagaggctgg
     2161 aaggtatcga agcctcgcgt gaagaggtcg agcgcgcggt cgaagagctc gaacaggacg
     2221 gcatcatcgt cgccgtccgc ggcaagcgtt actcgctgct cgagttcacg ccgtaccacg
     2281 ccggccgcat ccgcgtgcat ccggacggct acggcaccgt cttcggcggc ggcgaagagc
     2341 cggatatttt catcgaccgc cgctcgatga aaggcgcgat gaacggcgac ctcgtcgtcg
     2401 tccgtgtcga caagcacagc ccgaagttcc acaccatccg gcagcggaag ctgatcatcg
     2461 gcgaagtcac gaccgttctg cgccgcgccc accgcacagt cgtcggccgc tttcacgccg
     2521 atcccgaaca gcctttcgtc acgccgttcg actcgcgcct cgatcacgac atcgtcatcg
     2581 accgcaacgg cacgatggac gcgcgcgacg aagagatggt gaatgtcgag atcgaccgat
     2641 accccgaccg caccagccac atcgcgcgcg gccgcgtcgt cgaagtgctc ggcttcatcg
     2701 gtgagcccgg cgtcgacatc gagctggtca tccgcaagct gcagatcccg catcgcttcc
     2761 cacccgaggt gttggcggct gccgacgcca ttccggatga ggtgacggcg gcggagatcg
     2821 cgaaacgcgt cgaccttcgc gagcacaaca tcgtcacgat cgacggcgag accgcgaaag
     2881 atttcgacga cgcggtcgaa gtccagctgc tccccaacgg aaactatctc ctcggcgtcc
     2941 acatcgccga cgtttcgcat tacgtcaccg aggggagcgc gctcgatgag gaagccttcg
     3001 agcgcggcac gtcggtctac ttccctggcc gcgccgttcc gatgctgccg gagcgtctgt
     3061 cgaacggaat ctgttcgctg aatccgcgcg tcgagcggct cacgttctcc gtcgacatcg
     3121 agatcgacag gaagggacgc ttcgtcgacc gcaaggtcta tcgctccgtg atccgcacga
     3181 aagagcgcac gacgtacacg aacgtgaacg cgtttctcgt caacgaaggc gtggcccagc
     3241 cgagcgggat gccgccgcac ggcgtgatcg ttccgccgcc ggaggagttg cgggcacgct
     3301 acggctatct cctcgacgac ttcacgcgca tgcacgcgct ctacgacatc ctgcgtaaac
     3361 gccgcgaagc gcgtggctcg atcgacttcg atctgcccga ggccgaggtc attctcgatt
     3421 cggaaggggt gatcgatgcc atcaagccga ccggccgcaa cgtcgcgcac cgcatcatcg
     3481 aagagttcat gctcgccgcc aacgaagtgg tcgcctccga gctcgtcttt gccaatcaac
     3541 ccggcatctt ccgcgttcac cagcagcccg acccgcagaa gctcgaggat ctgcgcgaga
     3601 ttctgaagga gttcaagctg acgctgcgcg gcgacctgga agagatgcgg ccgggcgagt
     3661 tgcagcgcgt gatgaagtcg gtcgaaggga cgcccgaaga gcgcttcctc tcgaacatcg
     3721 tgctccgttc gatgaaacgt gcgtactact ccgaagagaa cgtcggccac ttcgcgctgg
     3781 cgatcgccaa ctatgcgcac ttcacgtcgc ccatccgccg ctatcccgat ctcatcgtcc
     3841 accgccgcct cggcgaaatg gtcgataagg gaccgctgta cggcgagcgg ttggcggaag
     3901 tcgagcgcgc ccacccgttg tacgctgcgc aatcgagcga ccgggagcgg cgcgcggacg
     3961 aggcatcgcg cgaggtgctg gagtggaaga aggtcatctt catgcgcgac aaggtcggcc
     4021 tgaagttcac cggcatcgtc accggggtcg ccgcgtttgg cctcttcatc gagctcgatg
     4081 agatcttcgt gcaggggatg gtgccggtgg cgacgatcgg cggcgacttc tgggtctttc
     4141 gcgagcgcga gcaccggctg cgcggcgatt ccaccggccg cgagatgcgc ctcggcgaca
     4201 aagtgacgat cgaagtgaag tcgatcgacg aagaccggca ccagctcgag ttccgcctca
     4261 tcgagatcgg tggttcgcct attgcactgc gcgagcgata gttagccgat aatcccagcg
     4321 gaaggaccgc atacgaatgg ccgatcaaaa cgaccttgcc acgcagatca ggaatctcgt
     4381 cgatcagcgt gtcgccctcg aaacgaaact gcagaacgag ccgggggagc tctccgaatc
     4441 caacgcgcgc aagtacgacg ccgcgtacaa cgacttgcag cgcaagctcg ccgcaatctt
     4501 caagcagacg gacgaggagc agccgcgcat cacgctgatg ctcgagctga tgcgcatcct
     4561 cgaaaaccgc ctcgtctacc tccacaactt catcgtcgac gcaacctccg acaaccgcgt
     4621 cttcgcccag ctcgtcatgg agttcattgc acaattgctc gcgcaacgaa gcaacgtcct
     4681 cacgaatctg gaagcgacgt attacggagc ggtggccaca ctttacgctg gcgatttagc
     4741 cggcgcgcgc aagggcttcg ctgcagcatg tgcttcggaa gagtccgacg aagccaacga
     4801 catcaaatac aagtcctacg tcatcctcgg ccacctgtca cacgaggagc gtgattacgc
     4861 caaggcgaag gatctgcacg accggtcgat gcgctattcg cagaacaaca atgtgaccgc
     4921 gcaggcgctg gcgctcaaag cgctgaattc ctacgccctc ggcgaccgcg ccgacgcgct
     4981 gcacctcttt caggagtcgc tcgagctctt cgacgccaat cagccattct tcaactcgta
     5041 cttcttccgc aacgcgcttc tcttctgcgg cgcaatccat ttcgatggca aggattacgc
     5101 gagcgccgaa ccgtattacc gccgcgtgct ggcgaacgtc gagcagacct cctacgatta
     5161 tttcgacgcc caaacccact tgggccgcat cgcctacacg caacagcact acgacgatgc
     5221 cgtcgcatgc ttcgagaaag ccgtgcaggc ccagaagctc agcgaaaacg aatcgatcgt
     5281 cgacacttgg ttctggatcg cccgcaccca cctcaaacgc aacgactccg ccgccgcccg
     5341 cacctacctg gaaaagatcg ccgcctcaga tgtgaaatac gaaaagaagg cgcaagcggc
     5401 agagctgctg cagaagatcg cgtagcgaac tgaaccgctg taaagtggtt cgcggggacg
     5461 ctggataccc gctgttttcg cagataccag gttgtctgta cactctttaa ggaccttcat
     5521 taaacaataa agtgacgacc atcacgtcat caggtgaccg gccgatctct cgattacaaa
     5581 aaatcagcta agtcagggta gggattcccc tccgcccgtc actcctagag cagaaccacg
     5641 attctctgct cctcacgtct tgaaaatctg ccgcaattgg aggcgtctgt gaaaaccgtc
     5701 ctcgtagcaa tcagtgtgtg tcttctttct gtcctcaacg cgaccgcgca atggatcgat
     5761 tgtacaaacg ctccagcggg ctcgaaaact tgcacgcaaa cgtccccggt cggcatcgga
     5821 acgttgtcga cgcagtatca actcagtgtt ttgggaaccc aaaataccga tggaatacaa
     5881 atcaatagtg gcaacgcgac gatgttgaag ttggatacta accgaaccga cggattgaac
     5941 tatgctttcg gactcaatcg tctcgcggcc ggagattttg cactctatga gggaacgtcc
     6001 ccgagaggcg actcgtttgg cggaactcgg agattgtata taaactcggt tggcaatgta
     6061 tcgctacaac cagatagtgg caacgtcggc atcggtacga catcgccgag cgtcagattg
     6121 tcgctaggtg gcgggccagg tggtattaag caactgctat acgacgagcc gggcttcagc
     6181 ccggactaca aaatggggtt tggcgtcaac cttgcgaatc ctggtgtcag ttcgtcaatc
     6241 gacgttttga tgggcttcgg aacaggggct gacaacagtt tcaatgtggt caaacccaca
     6301 actgggtggc cgtattatac ttcaacgtac acaaattcta cgttcacaaa gctcctgacg
     6361 gtgctggaaa acggcaacgt cggcatcggc accttctcgc cggaatcgat tcaatccggt
     6421 ggcccagcgc ttcatctgta cggagcggga ccgtcgttgc gcattcagga cacagcgtcc
     6481 aacggcgatt ggaatctgtt cgcgcgaacc ggcgcttcga cgaacctttt cagaatctac
     6541 gacaatgccc acagcgctga tcgtctgacg atcgacgcgt caggcatcgt aaacgtcggc
     6601 gtcgcgctaa ccgtgggccc cgcaggcctc attcatggta acagcctcaa cgtcaacggc
     6661 gatgtcacgg tctccggcaa ccttgcggcg cggtttcagg atatcgcgga gtgggtgccg
     6721 gtgaccgagt cgatgaccgc agggaccgtt gtcgtagtga gcaatgacgc gtcgaatacc
     6781 gtggccccat cgctccacgc gtacgatacc ggggtggccg gagtgatttc agcacaaccc
     6841 ggtctgaccc tgggcgaggg atcgacaacg aaggcaaagg ttgccactac gggccgcgta
     6901 agagttcgcg tggatgcgac cacacacccg attgctcgag gcgacctcct ggtcacgagc
     6961 gacaagcccg gaaccgccat gttctccgag gcgctcgatc ttggcggcgt caagatccac
     7021 cgccccggca ctctcatcgg caaagcgctc gaaccgcttg ccaaagggca gggcgagatt
     7081 ctcgtcctgc tcagtcttca gtaacgagga gttgatgatg aaaatctccg cccgcttgtt
     7141 gactgtcctt ctggttctga gcatacctgt gacgggtcta gtcgcggcac cggtcgttcc
     7201 ggcaattacc ttcgattcca cgcacgtgat tgcgacgggg tttcactccg ctcgcgacat
     7261 cgtcattttc ggcgtgggaa cgggaccggg cccttacttc ctgcgcctca tgcggttcac
     7321 ggatacgctg accgcggatt cggcggggac ggtgcggtac gaaatcgcaa acggtgttcc
     7381 cgaacgctcc gtctggttcg cggtcgatac acagacaccc gactacgccg ttggttcacc
     7441 tcagcctgcg ctactccacg ccacgctgag cgcaccagga attcggcggg ggactcagcc
     7501 agcagcagat acggtcgatc ttgaccggcg gttggtggat cttctgctcg tacgcccggg
     7561 ctccggagcc tgggcgggtt cgtgcgggcg caacagtctg aaggaccgca atcgagggaa
     7621 gagcggagcc atgcagtttt cggcggccga catcgacgcg gcgccgaaaa ccatcggccg
     7681 cccaagtacg atcttaccct cggatctcat cgtcatcatt gactcggaaa cgctcgagta
     7741 tttcatcggc agcccgaggc agctctgagg tccgcatgat gcgcaatcgc ctattgttcg
     7801 ccctttctct ttcaatcgtt attgcactca gcctccacgc acagacccat ccgaatatcg
     7861 agaagggttt tgccgcagac aagatgtacc agttcggcga catcgatcac gttaatcttt
     7921 tcaacggaaa tctttccctc acaataccaa tcggttccac gccggtcagc gaccgtctgt
     7981 ccttgtcgct tactctcgtt tacaacacga agctgtggac ggcactagat aaagaataca
     8041 acgtcggcgg ccaaactgga cgtgcgcctc tgtacacacc ggaacgacgc tccaacgccg
     8101 gtctcgggtg gaccctgacg ctggggcgtt tgcttgaccc atcggagacc gacgttaact
     8161 ccatcatcaa tggggcaaca cgcttcgcgt acatgagtcc ggatggaagc gaccggctgt
     8221 tctacgacac actccacgac gaggagacgg ctactgccgg cgtgctgtat tcccgcgata
     8281 acacctacct ccggttgtcc gatggcgggg caggaaagat cctcgaatca ccggatggca
     8341 cgatccggga gttcgagtcc tacatcgata gcatcactaa tacgacgtgc ccaactccgc
     8401 catgcggctc gtacacgcgc tggcgcttga caacgattcg cgaccggttc ggcaatcaca
     8461 tcgacatcga ctacagcacg gcggggcagt ggagaatcac ggatcactac ggacgtctta
     8521 cggttgtcca tttcgcggcg catgcgtcgg acgtcgacgc cgaatacctc cagcaggtca
     8581 gctcgattga tgtccccact acgggtggag gcaccgcctc gtacggtttc agctacgagg
     8641 gagtggacga tccgggcaat cctcaggaca agtgggtgaa aaccggtgct tgcgccgtgc
     8701 cacatcccga tacacctgtg ggctaccgtc tgcccctcct caaatcgctg agccttccgg
     8761 acagtaacgt gtataccttc acctacactg gcccgattgg cgacacgctg gacagcgttg
     8821 cacctggtgg taacgaggct tgcacggcgg gcggcctcgc gtccgtcacg ctgcccacaa
     8881 aagcgaaaat ttcctacgaa tatgacgttt acgacctccc gacactggcg tgccatgatg
     8941 atcctgatcc tatcgctgag tcctccggaa tcaagacccg gacggttcag gatccgttca
     9001 acgccagcaa cgaccaagct tggcactata cggtggcggg ggagcaaagc tttacctatt
     9061 tgggaggtca aatgacttgc ccgggtccta tccaggtgga cgctggcccc tcccagtaca
     9121 tcaatacggt gacggttagc acatcggccg agccggtcgc ccagaagact cgctactact
     9181 tctctgtcta tcacggcaag ctttccgacg ctaatggcac tacggtccac gactacgggt
     9241 tgccattctc gcgcatccat cctttcgata cgacgaagac acggtttctc tcgaccgagg
     9301 tcctggacgc atccgataca cccaagaggt cgacctatct ctcctacatt tacgaaccgg
     9361 ccgggtccgc tgtgacttct tctgccacct acgcgtttaa cccccgaatc accggcagcc
     9421 gcacggtctt cctggacgat gagaagacgc caggcaatcc gtattacaac gacacggcct
     9481 tttccgaatt cgacggcgta gggcactatc gcaccaagac ggtcagcgga aacatggatg
     9541 gatcgacacg tatcatcacg acgtcgtaca acaccgtcga tccgctcgtg aacccgggcg
     9601 cgctggaaac cggctgcttc gagtcctgcg ccggcggagt tgcgttcacc ccgccgctga
     9661 cgagcgcagc gtgggtactg aacttgtacc caacgtcgac caccagcgag agcggtgcgc
     9721 agggttctcc ctccgtgcaa gaggcctgct tcgacaagac caccggtttt ctcaacggca
     9781 cccgcactcg cgcgggcgca agccgcgaca gcaaagactt gatcacggtg tttggatcaa
     9841 gttcagactt cagcctgacg ggtaatcttc atggcgcggt cacgtccgag aagtattatg
     9901 ggggagacgt taatccgttg acttcgagct cgagccagac cctgtgcgac acagtgacca
     9961 gctctacggc tcccggcacg ctcggctacg acatccggca cacttacgtg aacgcaatgc
    10021 cagcgatatc gcagtactac acgaatgggg cgtctgtggg cttcaagttc ctcgaccagg
    10081 acaccgatta ctccacgagc ctggtcgtgc actcgcgcga caccgcaggc gttcagaccg
    10141 cctatacgta cgatagttcg cggcgcgttt cgagtgtgca ggcaggcggc gatccgatca
    10201 ttaactacac ctacacaaat gcgacgtctt ctgccggcgc gaatgtgacg atcgaccaga
    10261 cgtcaacgac aagtggcgcc ccgaaggcgt cgttcgacta cgacggcctg ggccggctcg
    10321 tcctgcaaag ccgcactctg ccgcgggcat ccctcggcga cgcgactcga tggacgcggc
    10381 aaaagacggt ctatgacgcg atgggccgta agacccaggt gtcggaatgg gaggaatcga
    10441 gctcaccgtc tcattttact gtctcttccg acttcgacag gttcggccgt cctggaacga
    10501 tcaccaggcc tgacagctca acgacgacgt tcgcctacca ggggggacgc cgcatcgacc
    10561 gcaccgctca cattcacacc cagagcgggg ccgacaccaa cgcaaccaca acggaggtct
    10621 acgacctcca tggccggctc tataaggtca acgaaccggc caacaacacc atcgctacgt
    10681 actcatac
//
LOCUS       DATLVW010000616         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_275579, whole genome shotgun sequence.
ACCESSION   DATLVW010000616 DATLVW010000000
VERSION     DATLVW010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275579"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..969)
                     /gene="pssA"
                     /locus_tag="VNN25_18215"
     CDS             complement(163..969)
                     /gene="pssA"
                     /locus_tag="VNN25_18215"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXH93522.1"
                     /translation="MQRQDVRKNLRRGVYIVPTSFTMLNIFFGFRSIVNSLRGAEAIA
                     IGRTDLAIHAFEIACVSLLIAAVFDTFDGLVARQLGATSEFGKEYDSLADVVTFGCAP
                     AVLVYAWGLHSLGKLGVGIAFLFLVSVSLRLARFNVMTGKTDYRYFVGLPSPAGALTL
                     AAVIYYAPAPVNDHQFATVIMILTGLTAFAMVSPIRWRAQKGINFQKGRSLMYIVVIA
                     AVLAVAWAWPRPFLLFFAIAYLASGPLIRLWTIAFPPAIPAEVLPAPPTA"
     gene            complement(969..1613)
                     /locus_tag="VNN25_18220"
     CDS             complement(969..1613)
                     /locus_tag="VNN25_18220"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HXH93523.1"
                     /translation="MSKVPWAPEGWLFLIPVLILTAAALLIQWTIAAIVLGLLALFLI
                     NFFRDPRRTGSEHPVDVLSPADGTVVQIKDIPDGDIWPGLTRQVSIFMSVFDVHVNRA
                     PISGRIIHYRYNPGKKIAAFAEKSSTENEQNLIVLEDKHGAKLAFKQIAGLLARRIVF
                     DKKEGDEVVRGERVGMIKFGSRVDMFFPAGAVIKVGMRDKVKVGLTVVAELGAK"
     gene            1794..2030
                     /locus_tag="VNN25_18225"
     CDS             1794..2030
                     /locus_tag="VNN25_18225"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HXH93524.1"
                     /translation="MSNQIADIQRILSTEDEEFRKWLDEHHECESRLGELIAKNEVST
                     DEELEEKTLKKRKLQLKDQMAARIRTYENQNAMA"
     gene            2231..3799
                     /locus_tag="VNN25_18230"
     CDS             2231..3799
                     /locus_tag="VNN25_18230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH93525.1"
                     /translation="MPRSLTTAAVFLLVLAALITPMQRDLFVGDETKYGQVVREMRAT
                     GAFFLPTLNGAPFTHKPPLHFWLIDLLSYPLGVYSTWAFVLPSLMGLVFLLWLMWRMG
                     GPLAAFICGSSMMVWTSAQSARMDVTFTAFIVLALWQLERFFERDDFRALLWCGIAIG
                     VAILIKGPMAPVIAIVLFGLEWWRRRRAPRGNYAPALLAMIAIPLLWFVPAMILGGGA
                     YTHDVIVKQTVGRAIASWVHNAPPWFYVAHLPASLFPWFFLACVAVVALWRTQRYLVN
                     WIIAVIVPYSIMSSKLDVYMMAMIPPIALLIARYIAEREARTESEARTERESVERRRP
                     AGWSAAGPAANLIANALIALIAIALFMNPTRIKGPESALIALPAVRTLLIVTAVAAIL
                     AFAVTLRASLTTSTIATGLVPIVSMAFIAIALMPVVNDLVSTRPLVSALQQQQVPPEQ
                     IALYAAPYLWTRDFPRQYERVVYADPDTLTRTKPAMVVTARAHANEIASALNGYHVVT
                     SLRMLGKWFDVYRQ"
     gene            3783..>4242
                     /locus_tag="VNN25_18235"
     CDS             3783..>4242
                     /locus_tag="VNN25_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93526.1"
                     /translation="MSIDNKRRLAAAAFIALLALSVFWPSPAIAVNNVCCHAQLGVDD
                     LSFLGREAPAWDVAFWFIAGLFALLILHPSTGYAAHDFAEAWTLVRATRLRLRWIDAI
                     AAICAAVLIALIWRLADASVTAWAERIQSSGIQDTIRFANRFGGGMNPPMV"
BASE COUNT          769 a         1373 c         1282 g          818 t
ORIGIN      
        1 gacgatggca aggcggtagc cggagggctt tttgggctcg gtcatgtgcg gcgcgagttg
       61 tagcacaccc gttgaaggct ttttcacgcg gagccgcgga ggagcggaga aaacctccgc
      121 gttctccgcg tctccgcgtg aaatggtttt caagagcgag atctacgcgg ttggcggcgc
      181 tggcaacacc tctgccggaa tcgctggcgg gaacgcaatc gtccacagac ggatcagcgg
      241 ccccgaggcg agataggcga tggcgaagaa aaggaggaac ggacgcggcc aggcccaggc
      301 cacggcgagg actgccgcga tcaccacgat gtacatcagc gatcgtccct tctggaaatt
      361 gatgcccttc tgcgcgcgcc agcggatcgg agagaccatc gcgaaggccg tgaggcccgt
      421 gaggatcatg atcacggtcg cgaattgatg gtcattcacc ggcgccggcg cgtagtagat
      481 gaccgcggcg agcgtcagcg ccccggccgg gctgggcagg ccgacgaaat agcggtagtc
      541 ggtcttgccg gtcatgacgt tgaaccgcgc cagccgcagt gagacgctga cgaggaagag
      601 gaaggcgatg ccgacgccga gtttgccgag ggagtgaagg ccccacgcgt agacgaggac
      661 ggcgggggcg cagccgaacg tgacgacgtc cgcgagcgag tcgtactcct tgccgaactc
      721 gctcgtcgcg ccgagctgcc gggccacgag tccgtcgaat gtgtcgaaca ccgcggcgat
      781 cagcagcgaa acgcaggcga tctcgaatgc atggatcgcc aggtctgttc gcccgatcgc
      841 aatcgcctcg gcgccacgca ggctgttcac gatcgaccgg aagccgaaga agatgttcag
      901 catcgtgaac gaggtgggga cgatgtagac gccgcggcga aggttcttcc ggacgtcctg
      961 ccgctgcatc acttcgctcc gagctcggcg acgacggtca atccgacctt cactttgtcg
     1021 cgcatgccga ctttgatgac ggcgccggcg gggaagaaca tgtcgacgcg cgagccgaac
     1081 ttgatcatgc cgacgcgctc gccgcgcacg acctcgtcac cctctttctt atcgaacacg
     1141 attcgccgcg ccagcaggcc ggcgatctgt ttgaaggcca gctttgcgcc gtgcttgtcc
     1201 tcgaggacga tgaggttctg ctcgttctcg gtcgatgact tctcggcgaa cgctgcgatc
     1261 ttctttcccg ggttgtagcg gtagtgaatg atccggccgc tgatcggcgc gcggttgacg
     1321 tgtacgtcga agaccgacat gaagatgctg acctgccggg tgaggcccgg ccagatgtcg
     1381 ccgtcgggga tgtctttgat ctggaccacg gtgccgtcgg cgggggagag gacgtcgacg
     1441 ggatgctcgg agcccgtgcg gcgcggatcg cgaaagaagt tgatgaggaa gagggccagg
     1501 aggccgagga cgatggcggc gatcgtccac tgaatcagca gcgccgccgc tgtcaggatg
     1561 aggactggaa tcaggaacag ccacccctca ggcgcccacg gcacttttga catttcgcgg
     1621 atggtagtgc aaaggcggag ccgatgggaa ggcgtgcgcg ctgccgtcag gaattggaac
     1681 ggcgcgcaca ttgcagtctc aactgtctga tatggaagct acttagcgtg atcgaaagat
     1741 ggcctccaga gtgctacaat ttccagcgaa ggtacttcga aggaggatag aggatgtcga
     1801 atcaaatcgc tgacattcaa cggattctca gcaccgaaga cgaggagttc cgcaagtggc
     1861 tcgatgagca tcatgaatgc gagtcgagac ttggtgagct cattgcgaag aacgaggtct
     1921 ccaccgatga agagctcgaa gagaaaacgt tgaagaagcg aaaactccag ttgaaggacc
     1981 agatggccgc ccggatccgc acctacgaga atcaaaacgc gatggcgtag cggatcgacg
     2041 acacacctga tcaactgagc cgctgcaagg cggctctttt tgttggtcca gagatcggag
     2101 acaagtgacg agagatcaga gactgagatc tcacgcccgg cttcaacagc ttggtctctt
     2161 atctcttgtc tcttgtctct gatctgtgct tcacgatctc tgcttctctc cactaatcta
     2221 ctattcacca gtgccgcgct ccctcaccac cgccgcagtc tttctgctcg tacttgccgc
     2281 gctcatcacg ccgatgcagc gcgatctatt cgtcggcgac gaaacaaagt acggtcaggt
     2341 cgttcgcgag atgcgcgcca ccggcgcgtt ctttctgccg accttgaacg gcgcgccgtt
     2401 cacgcataag ccgccgctgc acttctggct catcgacctc ctctcctatc ccctcggcgt
     2461 ctactcgacg tgggccttcg ttctcccgtc gttgatggga ctcgtctttc tgctctggct
     2521 catgtggaga atgggcggtc cactcgccgc gttcatctgt ggctcgtcga tgatggtctg
     2581 gacctcggcg caaagtgcgc ggatggacgt caccttcacc gccttcatcg tgcttgccct
     2641 ctggcaactc gagcgcttct tcgagcgcga tgattttcga gcgctgctct ggtgcggcat
     2701 cgccatcggc gtggccattc tgatcaaagg accgatggct cccgtgatcg cgatcgtgct
     2761 cttcggtctg gaatggtggc gccgccgccg cgcgccgcgc ggcaactacg cgcccgcgct
     2821 tctggcgatg atcgcgatcc cgcttctttg gttcgtcccc gcgatgattc tcggcggcgg
     2881 cgcctacacg cacgacgtca tcgtcaagca gaccgtcggc cgcgccatcg cctcatgggt
     2941 ccacaacgcc ccgccgtggt tctacgtagc gcatctcccc gcatcgctct tcccctggtt
     3001 ctttctggcg tgcgtggccg tcgtcgcgct ctggcgcacg cagcgctacc tcgtcaactg
     3061 gatcatcgcc gtgatcgtcc cgtactcgat catgagcagc aaactcgacg tctacatgat
     3121 ggccatgatc ccccccattg ctctcctgat cgcgcgctac atagccgagc gcgaagcacg
     3181 aacggagagc gaagcgcgga cggagcgcga gtcagtggag cgccggcggc ccgccggctg
     3241 gtccgccgcc ggcccggcgg ccaacctaat cgcaaacgcc ctgatcgccc taatcgccat
     3301 cgcactcttc atgaacccaa cccgcatcaa aggccccgaa tccgccctca tcgctctccc
     3361 tgcagtacga acactcctca tcgtcaccgc cgtcgccgcg attctcgcct tcgccgtcac
     3421 cctgcgcgca tccctcacaa catcgaccat cgccaccggc ctcgttccga tcgtgtcgat
     3481 ggccttcatc gccatcgcgt tgatgccggt cgtcaacgat ctcgtctcga cgcgcccgct
     3541 agtcagcgcc ctgcagcaac agcaggtgcc cccagaacag atcgcgctct acgccgcgcc
     3601 ctacctctgg acgcgcgatt ttccgcgcca gtacgagcgc gtcgtctacg cagatcccga
     3661 cacgctcaca cgcacaaaac cggcaatggt cgtgaccgca cgcgcccacg caaacgagat
     3721 cgcatccgca ctcaacggct atcacgtcgt cacgtccctg cgcatgctcg gaaaatggtt
     3781 cgatgtctat cgacaataag cgccgtcttg ccgccgccgc gttcatcgcg ctgctcgcac
     3841 tctcggtctt ctggccgtcg ccggcgatcg cggtcaacaa tgtctgctgt cacgcccagc
     3901 tcggcgtcga tgacctgtcg tttcttggcc gcgaggcccc ggcctgggat gtcgctttct
     3961 ggttcatcgc cggcctcttc gcgctcctga ttcttcatcc ttcgaccggc tatgccgcgc
     4021 acgatttcgc ggaggcctgg acgctcgtgc gggcgacccg cctgcgcctg cgctggatcg
     4081 atgcgatcgc cgccatctgc gcggcggtgc tcatcgcgct catctggcgt ctcgccgacg
     4141 cgtccgtcac tgcctgggcc gagcggatac agtcgtcggg cattcaggac acgatccgtt
     4201 tcgccaaccg tttcggcggc ggcatgaacc cgcccatggt cg
//
LOCUS       DATLVW010000617         7759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_70816, whole genome shotgun sequence.
ACCESSION   DATLVW010000617 DATLVW010000000
VERSION     DATLVW010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70816"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VNN25_18240"
     CDS             complement(<1..387)
                     /locus_tag="VNN25_18240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXH93527.1"
                     /translation="MSHDHSHNGQISRKLLVASVATGVFVAVELTVGIAANSLGLVGD
                     ALHNITDTLALLLALFAVRVERRPATSAKSYGWHRAGVLAAFINAAALAAFTIFLFVE
                     AIARLRTPAAVNSTAMLWTAVAGLVLN"
     gene            complement(453..959)
                     /gene="purE"
                     /locus_tag="VNN25_18245"
     CDS             complement(453..959)
                     /gene="purE"
                     /locus_tag="VNN25_18245"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013022442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXH93528.1"
                     /translation="MSWVCIIMGSDSDAEVMSQACAALDDAGVPYEMSVASAHRSPER
                     TKEVITAAERDGAAVFIAGAGMAAHLPGVVASFTTKPVIGVPLVGGALQGLDALLAVV
                     QMPPGIPVATVAVGGARNAGILAAQIIALTDEGLASRLREKRASMAEEVARKSAKVER
                     STRSVKKA"
     gene            complement(1686..2153)
                     /locus_tag="VNN25_18250"
     CDS             complement(1686..2153)
                     /locus_tag="VNN25_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93529.1"
                     /translation="MLNNLVVQTSEEPDSKRPFCVLVVDDDPSIRRMIVAALRRDGYT
                     FCEAPNGKEALDMMRSEHPSVVVLDLMMPVLSGWDVLQARELEPKLREIPVIIISANR
                     APEVATAVDKGICAFLPKPFDIGALSALVRSCLAPGSSTGVHATPVAEAEAPR"
     gene            2210..3298
                     /gene="mnmA"
                     /locus_tag="VNN25_18255"
     CDS             2210..3298
                     /gene="mnmA"
                     /locus_tag="VNN25_18255"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXH93530.1"
                     /translation="MKIAVAMSGGVDSSTAAYILKSSGHDVVGLSMQMYDNLTNADTT
                     YGGCCTIDDLTDARRVAWKLEIPHFTLNLEQNFHEKVITPFVQSYLSGLTPSPCVLCN
                     THVKFDLFHQKARAIGAEKIATGHYARITRDAAGRFELRKALHLEKDQSYFLFELTQE
                     QLSGALFPLGEMTKPEVRDVAEEAGLTVARKKESYEICFVPQTDSYAAIVEREAGITP
                     GESSGEIVDTNGNVLGTHDGYYHFTIGQRRGLQVGGTADRMYVVDVNPFTKRVTVGDV
                     SDLERDELHAERVHWISGIAPSGPVEVQARVRSRAADIAATVTPLPDGRAHVQFAQKL
                     RAVAPGQAVVFYDGDLCLGGGWITSTRS"
     gene            complement(3295..4044)
                     /locus_tag="VNN25_18260"
     CDS             complement(3295..4044)
                     /locus_tag="VNN25_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93531.1"
                     /translation="MHWTEWLRIRRVVPLVTVSLALVVAAADLTGYLPHMNRDGVIVA
                     MLALLALDALVERVSIVDGIERRLANLESPTVLRDRSSLTQMTAMAVGAKEIVACGIT
                     LVSLVPTHHDFWKEKLRRGVNLRFMMIDPDSIAWQSWCESQRTPSPGDLTFALNSLAP
                     LLRESYPGQIEVRLSPQFLPVSIAGVDLGSDNGRMNVELVFTRTDLPNRPHVHLTKMA
                     SPQWFDFFAEIFEDLWTRSRPWIPPARSPSV"
     gene            4702..5169
                     /locus_tag="VNN25_18265"
     CDS             4702..5169
                     /locus_tag="VNN25_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93532.1"
                     /translation="MSTDNNTAKTGPADDPVAARRVFFSEASATMRHYSNLQFAIQTL
                     YFASIGALGSVAFVTERSYVLLGPETVGTWACLAAVVLTLVFFLLTLSCERHRKHFEA
                     KALRLERDMSEDVVDGVPASKVVGANTTMNFFYIVNVVFWTCILAIRWRLIVV"
     gene            complement(5238..5717)
                     /locus_tag="VNN25_18270"
     CDS             complement(5238..5717)
                     /locus_tag="VNN25_18270"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXH93533.1"
                     /translation="MLRYTFTDTPIGAILIAGDSESIVEIHFAGAKPKSDWIRDDDAF
                     PEANDQLRAYFAGELQTFSLPLAPQGTEFQMSVWRALQQIPYGETTTYSTIANRIGRP
                     AAIRAVGAANGANPIPIVIPCHRVIGANGSMTGFGGGIDVKRKLLALEARVAGQTLF"
     gene            complement(5824..6573)
                     /locus_tag="VNN25_18275"
     CDS             complement(5824..6573)
                     /locus_tag="VNN25_18275"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HXH93534.1"
                     /translation="MVKLAYRSPYDWNAILRFLRPRAIAGLETIDDVAYMRGSVRVTH
                     DAARNAINVSDKSAADQVRKIFDVDADIEAIEKHFARDPTLGPLVRAHRGIRVPGTWD
                     SFEMAIRAIVGQQISVAGATTLITRIATRHGIRPLSLMRARTNPGMPQARWNSIRGLA
                     KAVARGEVSFTRGATLEESIERLTALPGIGPWTAHYIAMRALREPDAFPHSDLGIRKA
                     AGTTSDRELLARAEAWRPFRSYATMLLWRSL"
     gene            complement(6605..7666)
                     /locus_tag="VNN25_18280"
     CDS             complement(6605..7666)
                     /locus_tag="VNN25_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93535.1"
                     /translation="MKTALLILLAIAICFSVYAASDSRNGVWTAELNTDGSALQVTLF
                     RGNHDSERRFGRGMNNVMGFELSLGSLTGLTSADVKSTAANVQFSMSRPAGKISFEGR
                     FSDGNGAGNFKFAPSEAFVREMATLGYTDFKDDELLVFATTDFTPQTVRDLRALGYQP
                     TQKEIIEAAIFRIDANAIREFNRLGYPNLTFRELVNFRVGNVNAAFVNGMKELGYTDI
                     SAHKLANLAILGVTPAYVRELRAAGLKDLSAHEAEQLRIGNITPAKITEYKRLGYALS
                     SHELSEFGIQGVTPKTIEELNAMGYGHLTAHQLVEMRIFGVTPDYIRKVEANGYKGVP
                     VEKLIKLRMSGADELMTGK"
BASE COUNT         1534 a         2324 c         2349 g         1552 t
ORIGIN      
        1 gttgagcacg agccctgcca cagccgtcca gagcatggcc gtgctgttca ccgcggccgg
       61 cgtgcgcagc cgcgcaatcg cttcgacgaa gagaaagatc gtgaacgccg ccagcgccgc
      121 agcattgatg aacgccgcga gaacgccggc gcgatgccat ccatacgact tcgcgctcgt
      181 cgccggccgc cgctccacgc gcacggcgaa gagagcgagc agcagcgcga gagtatcggt
      241 gatgttgtgc agcgcatcac caacgaggcc gagcgaattc gcggcgatgc cgaccgtgag
      301 ctcgaccgcg acgaatactc ccgtagcaac ggaggccacg agcagcttcc gtgagatctg
      361 accgttgtgg ctgtgatcgt gggacatgtt ttttgaacca caaagacaca aagatgacag
      421 aggttttctc ggtgtccgtt gtgtctttgt ggttaagcct tttttaccga ccgcgtcgag
      481 cgctcaacct tcgcagattt ccgagcaact tcctcagcca tcgacgcccg cttctcacgc
      541 agccgcgaag ccagcccctc atccgtcaac gcgatgatct gcgccgcgag gatgccggcg
      601 ttccgtgcgc ccccaacggc aaccgtcgct accggaatcc cgggtggcat ctgcaccacc
      661 gcgagcaacg cgtcgagccc ttgcagcgca ccacccacga gcggcacgcc gatcaccggc
      721 ttcgtcgtga acgacgccac cactcctggc aggtgcgcag ccattccggc tccggcgatg
      781 aacaccgcgg cgccatcgcg ctctgcggcg gtgatgacct ctttcgttcg ctccggactc
      841 cggtgcgccg aggcgacgga catttcgtag ggaaccccgg cgtcatcgag cgccgcacac
      901 gcttgcgaca tcacctcggc atccgaatcg ctccccatga tgatgcagac ccaggacatg
      961 gcgagcgaat ggtatcaggc actcgtggct cagaggttgt gaagaaaccg cgctgaatcg
     1021 ttggtaccgc ggatccggac gttgtgccag gcgcgctcct tcagtgaagc cttctgatga
     1081 gatgaccacc gatcgtggag acgacgtctc gacgttgtca tattcgatgt caacgtcgtc
     1141 gttttggatg cgaaattgtc atccccggag gtcatcgatg acatttttta tgcaaatttt
     1201 gcatcctggg agtcatcgat gacatatttt agtcatcgat gacatcgctt aagcaaattt
     1261 tgcatcttgg aagtcatcga tgacattttt tgagcaaaat ccgcatcccg gaagtcatcg
     1321 atgacatctt ttaagcgaaa tcggcatcct gaatgtcatc gatgacatct tttgttcgag
     1381 attgtcattc ctcgatgtca tcgtcgtcat ttcttatttg gaatcgacat cctggatgtg
     1441 aacgtcgtca ttctccactc gaaatcgtca tcatggatgg cgacatcatc actccttgat
     1501 ggctcgatgt ctcgacgtcc tcgaaatcgt cgctgacgtc agtgacgatg tcctcaacgt
     1561 ccggaaaatc gtctcaacgc tgaggaaatc gtctcaacat catctagatc gtcctcaacg
     1621 ttggcagtca aatgccgtga caggacaaga ggtactcgaa gcaagcgagt cacagctgcg
     1681 gcggctcagc gcggtgcttc cgcctcggca accggcgtcg cgtggacgcc cgtcgaactt
     1741 ccgggcgcca ggcagcttcg aacaagcgca ctgagcgctc cgatatcgaa cggcttcggc
     1801 agaaacgcgc agatgccttt gtcgaccgcc gtcgccacct cgggagcccg attcgcgctg
     1861 atgatgatga ccggaatctc tctcagcttc ggctcgagct cgcgcgcctg cagcacgtcc
     1921 cacccggaga ggaccggcat catcagatcg aggacgacga cgctcggatg ctcgctgcgc
     1981 atcatatcga gcgcttcctt tccgttgggc gcctcacaaa aggtgtaacc gtcgcggcgg
     2041 agcgccgcca cgatcatccg ccggatgctc ggatcgtcat cgacgacgag cacacagaag
     2101 ggacgtttgc tgtcgggttc ctcacttgtt tggaccacga ggttgttcag catgcacgaa
     2161 aagtgcctcg cggaatatcg tactcgcagc ggtcgcttct cgaaaggcct tgaaaatcgc
     2221 cgtcgccatg tcgggcgggg tcgactcgtc gaccgccgcg tacatcctca agtccagcgg
     2281 acacgacgtc gtcggtctgt cgatgcagat gtacgacaac ctcaccaacg ccgacacgac
     2341 ttacggcgga tgctgcacga tcgatgacct caccgacgcc cgccgcgtgg cctggaagct
     2401 ggagatcccg catttcacgc tgaacctcga gcagaatttt cacgagaagg tgatcacgcc
     2461 cttcgtgcag tcgtacctga gcggcctgac gccgagcccg tgcgtgcttt gcaacactca
     2521 cgtgaagttc gacctctttc accagaaggc gcgcgcgatc ggcgcggaga agatcgccac
     2581 gggccactac gcccgcatca cgcgcgacgc cgcgggccgc ttcgagctcc gcaaggcgct
     2641 ccatctcgaa aaggaccagt cgtacttcct cttcgagctg acccaggagc agctctccgg
     2701 cgccctcttc cccctcggcg agatgaccaa gcccgaagtg cgcgacgtcg ccgaggaggc
     2761 cggcctcacc gtagcgcgca agaaggagtc gtacgagatc tgcttcgtcc cacagaccga
     2821 cagttacgcc gcgatcgtcg agcgcgaggc cggcatcacc ccgggtgagt cgtccggcga
     2881 gatcgtcgac accaacggca acgtcctcgg aacccacgac ggctactatc acttcacaat
     2941 cggccagcgc cgcggcctgc aggtcggcgg aaccgccgac cggatgtacg tcgtcgacgt
     3001 aaaccccttc acgaagcgcg tcaccgtagg cgacgtctcc gacctcgagc gcgacgagct
     3061 ccacgccgag cgcgtccact ggatcagcgg catcgcaccc tccggccccg tcgaagtcca
     3121 agcccgcgtc cgctcccgcg ccgccgacat cgccgcaacg gtgacgccgc tgcctgatgg
     3181 ccgcgcgcat gtgcagttcg cgcagaaact tcgcgcggtg gctcccgggc aggcggtcgt
     3241 tttctacgat ggagacttgt gtttgggtgg ggggtggata accagcacca ggagttagac
     3301 cgacggagag cgtgccggtg gtatccaagg tcgcgacctt gtccacagat cctcaaagat
     3361 ttccgcaaag aagtcgaacc attgtggcga tgccatcttg gttaggtgga catgcggacg
     3421 attcggcaga tcggttcgcg tgaacacaag ctccacattc atccgaccgt tgtcagaacc
     3481 taagtcgact ccagcgatgg aaacaggtag aaactgaggc gagaggcgta cttcgatttg
     3541 tccggggtaa ctctctctca gtagcggtgc gagggagttg agcgcaaatg taaggtcgcc
     3601 gggacttggc gttcgttgac tttcacacca agactgccaa gcgatcgaat ctggatcgat
     3661 catcatgaag cgaaggttta cgccccgccg tagcttttcc ttccagaagt cgtggtgtgt
     3721 cggaacgagt gacaccagtg taatgccgca tgcaacaatc tccttggcac ctaccgccat
     3781 cgcagtcatc tgcgttagcg aactccgatc tcgaagtaca gtgggagact ccaagttcgc
     3841 aagtcgacgt tcgatcccgt cgacgatgct gacacgctcg accagggcgt cgagcgcaag
     3901 aagcgctagc atggcgacta tcacgccatc gcggttcatg tggggcagat aaccagttag
     3961 gtcggctgcg gcgacgacaa gcgcgaggga aaccgtaaca agcggcacga ctcgacgaat
     4021 ccgtagccac tcggtccagt gcatccgcgt aagctaccac ggaagaacga cgccgcgttg
     4081 cggcgaacaa tcctaaggcc ttcggcaccc gttacttcat ggcccgcccg accatcaccg
     4141 gtcagaaccc ttcggtggca gcgaaaccac cagtactcga tgctaccgcg acagactagc
     4201 cagggcaagg gaggctcggc tggccgtgcg cgccgcgccg cctcggtgct tatctcgtca
     4261 gtcggcctct gcgctcgggc caatcggcga ttgactctgc tgaacgccgg agttacggcg
     4321 taccatgtat tataatttgt ttcgcagtgg atctggaagg ccagctccgg tcggactatg
     4381 aggaggagcg catgaaggac gcggcgcgag ccacacgttt gcgcgaactc gatgaaatcg
     4441 aagagcatgc gcgcgatact ctcagaaagc ttgactatcc caagagcgct atcgactata
     4501 tgtggtgagg cgccacttgg ttttcgagta gcaggtggct gtggagaaaa tgggcatggc
     4561 gggcgttgga gatggtcgcc aaagagcgcg aagctctgaa atgactgcat tccgttgtgt
     4621 tggcatctaa cagtaattca ccgacggagg cctactatcg ctcaacggag ctcgcgaact
     4681 tgcgaagtgg ggacaaatac agtgtcgacc gataacaaca ccgcgaaaac aggccccgcc
     4741 gacgatcctg tcgcggcacg gcgagtgttc ttttcagagg cctctgcaac aatgaggcac
     4801 tactcgaatc tccaatttgc catccaaact ctctacttcg caagtattgg cgcgctcggc
     4861 agcgtcgctt tcgttaccga gcggtcgtac gttctgctgg gacctgaaac agtcggtacg
     4921 tgggcgtgtc tagcggcggt ggtgctaacg ctcgtgttct tcttgctaac actctcgtgt
     4981 gaacgtcatc gaaagcattt tgaggcgaag gcacttcggc tggagcgcga tatgtctgag
     5041 gatgtggtag atggcgtgcc ggcttcaaaa gtggtcggcg ccaacacgac gatgaatttc
     5101 ttttacatag tgaacgtggt tttttggacc tgcatattag cgatccgctg gagattaatc
     5161 gtcgtttgaa ccgaagtaac caagcactca actctcaggc ggtgccaacg tccatttccg
     5221 taacctgacg ttaccgatta gaacagcgtc tgtcccgcca ctctcgcctc aagggcgagt
     5281 agttttcgct tcacgtcgat tccgccgccg aagccggtca tggagccgtt ggcgccgatg
     5341 acgcggtggc aggggatgac gatggggatg gggttggcgc cgttggcggc gccgacggcg
     5401 cggattgcgg ctggtcttcc gattcgattg gcgatggtgg agtaggtggt agtttcgccg
     5461 tagggaatct gttgcagcgc gcgccagacg gacatttgga actcggttcc ttgtggggcc
     5521 aatggcaacg agaaggtttg cagctcgccg gcaaagtacg cgcgcaactg atcgttggcc
     5581 tcggggaagg cgtcgtcatc gcgaatccag tctgacttcg gcttcgcgcc ggcgaagtgg
     5641 atctcgacga tggattcgct gtcgccggca atgaggattg cgccgattgg ggtgtcggtg
     5701 aaggtgtagc gcagcatcgg ggacggttat tttatccgcg attacgcaga tgttcgcaga
     5761 tgagagcgac gcactcttga actatctgcg aacatctgcg tcatctgcgg atcaatcgta
     5821 aacctacaaa ctgcgccaca gcagcatcgt cgcgtacgac cgaaaagggc gccaagcctc
     5881 agcgcgggcc aggagctcgc ggtcgctggt cgtgccggcg gctttgcgga taccgaggtc
     5941 gctgtgcggg aaggcgtccg gctcgcggag ggcgcgcatg gcgatgtagt gcgcggtcca
     6001 gggaccgatg cccggcagcg cggtcagtcg ctcgatcgat tcttccaagg tcgcgcctcg
     6061 ggtgaatgag acctcgccgc gggcgaccgc cttggccagg ccgcgaatcg agttccatcg
     6121 cgcttgcggc atgcccggat tcgtgcgtgc gcgcatcagt gagagcggcc tgatgccgtg
     6181 ccgcgtcgcg atgcgcgtta tcagcgtcgt cgcgccggcg acgctgatct gctggccgac
     6241 gatggcgcga atagccatct cgaatgagtc ccaagtgccg ggcacgcgga ttccgcgatg
     6301 cgcgcggacg agcggaccga gcgtcgggtc gcgcgcgaag tgtttttcga tcgcctcgat
     6361 gtccgcatcg acgtcgaaga tcttgcgaac ctgatccgcc gccgatttgt cgctgacgtt
     6421 gatcgcgttg cgcgcggcgt cgtgcgtcac gcgaacggag ccgcgcatgt aagccacatc
     6481 gtcgatcgtc tccaggccgg cgatggcgcg cgggcgaagg aacctgagga tggcgttcca
     6541 atcgtacggg gagcggtagg cgagcttgac caccgccaca ttctgtcacc gcacacgagg
     6601 gtgactactt tcccgtcatc aactcgtccg cgccgctcat ccgcagcttg atcagctttt
     6661 cgaccggcac gcctttgtat ccgtttgcct cgaccttgcg gatgtagtcg ggggtcacgc
     6721 cgaagattcg catctccacg agctggtgcg ctgtgaggtg tccgtagccc atggcgttga
     6781 gctcctcgat cgtcttcggc gtcacgccct ggatgccgaa ttcgctcagc tcgtgcgaac
     6841 tgagcgcgta cccgaggcgt ttgtactcgg taatcttggc cggggtgatg ttgccgatcc
     6901 gaagctgttc ggcttcgtgc gcggacagat ccttcaggcc cgccgcgcgc agctcgcgca
     6961 cgtacgcggg cgtgacgccg aggattgcca gattggcaag cttgtgcgcg gagatgtcgg
     7021 tgtatccgag ctccttcata ccgttgacga aagcagcgtt cacgttgccg acgcggaagt
     7081 tgaccagctc gcgaaaggtg agattcgggt agccgaggcg gttgaactcg cggatcgcgt
     7141 tcgcgtcgat ccggaagatg gccgcctcga tgatctcctt ctgcgtcggc tgatagccga
     7201 gcgcacgcag atcgcgaacg gtctgcggcg tgaagtcggt ggtggcgaag acgagcagct
     7261 catcgtcctt gaaatcggtg tagccgaggg tggccatctc gcgcacgaag gcctcgctgg
     7321 gcgcgaactt gaagtttcct gctccgttgc cgtccgagaa gcgcccttcg aagctgatct
     7381 tcccggcggg acgcgacatc gagaactgca cgttcgccgc ggtcgatttg acgtccgcgg
     7441 acgtgagccc ggtcagcgag cccagcgaca gctcgaagcc catcacgttg ttcatcccgc
     7501 gcccaaacct cctttcggag tcgtgattgc cgcggaagag cgtgacctga agagctgagc
     7561 catcggtgtt cagctccgcg gtccagacgc cgttgcggga atcgctggcg gcgtagacac
     7621 tgaagcaaat ggcgatggcg aggaggatca aaagtgcagt tttcatcgga gttctccttc
     7681 gtttggtgat catctacgaa atagatcgta gttgtcaacg aagaacttcg tagaaagttg
     7741 cgtgtcatct cggttaacc
//
LOCUS       DATLVW010000618         5089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_523008, whole genome shotgun sequence.
ACCESSION   DATLVW010000618 DATLVW010000000
VERSION     DATLVW010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523008"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(558..2033)
                     /locus_tag="VNN25_18285"
     CDS             complement(558..2033)
                     /locus_tag="VNN25_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93536.1"
                     /translation="MCRPIIACWALLFVCAAVPQADASQDIRRPLGVYAKVDVETAIQ
                     GYPGSVTPSPAQLHSYLRALYADLLANPAISGITVGMHWDNIQLSETIYDWSYLDDAF
                     DDAVAARKPVQLIITPGFNSPQWLLAKIPSCDGLFTNGIASADCGSVTFEGFPQEQHS
                     DNKVLPLPWNSIYKGAWQNLLTQLNARYSANPAFVCIAVAGPTGPSDEMILPTTANDA
                     SIQPSGLLVDDTWAALIKHSFPDNSSYQGSDQVFIDEWKQVIDAYERIFSGVTLSVSP
                     DAGNDLPAFGRKVTPHPDNVLYVNDCAASIASNIGLMSCEAKAEILSYFVAVTGPNGK
                     VTRVGGMTASSPATLAAGDIGIGGVKLLTLFSPPPSPPLLGGAEFDHPVSGSSNFQDE
                     GCPSYPTVCAGLTVEEAAYHVLTIFFNGTPVAASYGGTPGDAHMHYLEVPYVDVQYAA
                     THPAPAVPSSIIGNMSLQDLLNKASHDLTVINAPRRRADRH"
     gene            complement(2326..4314)
                     /locus_tag="VNN25_18290"
     CDS             complement(2326..4314)
                     /locus_tag="VNN25_18290"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH93537.1"
                     /translation="MRQLRTVYLLAMLLSSTAIYGQQGASSGSSPADIIYARPGKLVS
                     VNGFRLNLYCMGSGSPTVVFDSGWGDWAPAWSTVQPEIAKWTRACSYDRAGTGFSEPG
                     PMPRTSVRIAKELRTALHRAGIGGPYILVGSAFGGDNVRTFADLFMDEVAGLVLDDAD
                     PVDLEPKAMQEESHRGQAHIPVDLRACRDLIAEHKPLPKVSSRPDRPPQSCAQRFFFR
                     GLPDAAWSPELNAKLLDLAQTKVAMYDAYASEMEQTPADEIYLQRHRRSFGSRPIRVL
                     TSGNHGVGHLERKPPDTPEHLKYEQETTLAQARWLELSSNSRQIFARNSSEYIQFDEP
                     DTVINAIRDVYDQTKQPSDHKTSAHKPATVFRDCSECPEMVVVPAGDFTMGSSDAEKS
                     WAASHGSNKASVADEAPQHQVSLPSFALGKYDVTRGEFAAFARETGYPAGDGCGRGRA
                     IFKWEKDPKSTWESPGHVQTDRDPVVCVSWQDARAYIAWLNRKAHRGGAGAAAANEPY
                     RLPSEAEWEYAARAGTTTKFYWGDDDAAAPVHAWFNANSGCEKIEGLYCENGQPHPVG
                     AKPPNAFGLYDMAGNVWQWTEDCYDNSYAGIPADGRANETPSSDPKARDSQGNCLRVD
                     RGGSWMFPAWLLRPATRERNPAGFRADIMGFRVARTLP"
     gene            4605..>5089
                     /locus_tag="VNN25_18295"
     CDS             4605..>5089
                     /locus_tag="VNN25_18295"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HXH93538.1"
                     /translation="MTFIRSDGVEEVSFPCMGLFYVGCKVVNTRNSKSAVVPRMMVDT
                     GPEATWIDSAVLAKIGIEPRKKDLQFQMANGSIITRSVGYAVLKVDESETVDEVVFAQ
                     KGDLQLLGARALEGLNLKVDSRRKKLVAAGPIVVAAAVPPQFGELIRVPIHEQKPRHK
                     G"
BASE COUNT         1011 a         1510 c         1559 g         1009 t
ORIGIN      
        1 agaaattctt gttttcttgc aatcaatcgt ccagcgaacg gactacccct gcagccgaaa
       61 tcaagagaat ccgaccggac ccgcggttgg cgctatcgtc aggcgatgtc ccgcctgatc
      121 gagctcgatc aggaacgcat cgccggggtc tgtcgcgctt cgaccgagta aaggtccgtg
      181 gaacactggc tgcagtgtct gccaggatgg ccagaccgcc cgatagccgt ggcattcgga
      241 tagtcgacta cgcgaagctg ttcgctgagc ctttacgtac ggataccctc gccggtgctc
      301 aaactcggtt tccagaatga cgtagtgttc gcgaggtgtt cgacgggtcc acctacggcc
      361 atccgaggac atcctgggat gtcccgggac cctcgtaaac cactgaatct acggacatcc
      421 taggatatcc ggggacgggg tctctagttc acacggtggg agtcaccggt tcgagtccgg
      481 tagcgcctgc caaatcgcaa gtgcttcgaa ggccgcgagt tgcgagactc gcggcctttt
      541 tcgttttgtc acggtcctca gtgtctatcg gcgcgccggc gcggggcgtt gatcacggtc
      601 aggtcgtggc tggccttgtt cagcagatcc tggagcgaca tgttcccgat gatcgagctc
      661 ggtacagccg gggctggatg cgttgccgca tattgaacgt ccacgtaagg gacctccaga
      721 tagtgcatgt gcgcatcgcc cggcgttccg ccgtaggatg ccgcgaccgg cgtcccgtta
      781 aaaaagatcg tcagcacatg gtaggcggcc tcctcgaccg tgaggcccgc gcacacggtc
      841 ggataactcg gacatccttc gtcctgaaag ttgctcgagc ccgagactgg atgatcgaat
      901 tcggctccgc cgaggagcgg aggagaaggc ggcggcgaaa acaacgtcag cagcttcaca
      961 ccgccgatac caatatcgcc tgcggcgagc gtagccgggc tcgaggccgt cattccaccg
     1021 actcgggtta cctttccatt aggaccggtc accgcgacga aataggacag gatctccgct
     1081 ttcgcttcgc aggacatgag gccaatattg ctggcgatgc tggccgcgca gtcgttgacg
     1141 tacagtacgt tgtcgggatg cggcgtaacc ttcctgccga acgcgggcag gtcattcccc
     1201 gcgtcaggac ttaccgaaag ggtcactcct gaaaaaatcc tctcatacgc gtcaatgacc
     1261 tgtttccact catcgatgaa cacctgatcc gagccctggt aactgctgtt gtccggaaag
     1321 gaatgcttga tcagggcggc ccacgtatca tcgaccaaaa ggcctgacgg ttggatggac
     1381 gcgtcgttgg ccgtggtagg aaggatcatc tcatccgacg ggccggtcgg ccccgccacg
     1441 gcgatgcaga cgaacgcggg attggcggag tatctggcat tgagctgtgt gaggagattc
     1501 tgccaggccc ctttatagat actgttccag ggcagaggga gcaccttgtt gtcggagtgt
     1561 tgctcctgcg ggaatccctc gaaggtcacg gaaccgcaat cggccgaagc gattccattg
     1621 gtaaagagtc catcacagga ggggattttc gccaacagcc attgcggtga attgaagccg
     1681 ggcgtaatga tgagctggac gggcttccga gcggcaacgg cgtcgtcgaa ggcgtcgtcc
     1741 aggtaactcc aatcataaat cgtctcggat agctggatgt tgtcccagtg cattccgacg
     1801 gtgatccccg agatcgcagg gttggccaac aggtccgcgt agagcgcgcg aaggtaggag
     1861 tgcaattgtg ccggcgacgg tgtgacagaa ccgggatagc cctggatcgc ggtctcgacg
     1921 tcgacctttg cgtagacgcc cagcggcctg cgtatgtctt gcgaagcgtc tgcctgcgga
     1981 accgccgcgc atacgaagag caatgcccag caagcgatga tcggtcggca catggtgctg
     2041 ctatcgatga gcgcgatcag tatagctttt cccgtccgga gtcgcgaccc ctcgactgcc
     2101 agttccttgc ccaccagctc ggtccaggcg gcgcacaccg aggagcatac gaccgggagc
     2161 aggactcttc gcgcccgcaa gactcctcga tgtggcaacc tcgaccgcga atcgcatcgg
     2221 cgccacgcgg cgatctgtct ctcaccggag gcgctgcatc gcgcgggcgc gaagcgccca
     2281 cgatagactc ggatcgatag aaaaacgaag atctgacccc ttccttcacg ggagcgttcg
     2341 cgcgacgcgg aagcccatga tgtccgcgcg gaagccggcg ggattgcgtt cccgagtggc
     2401 cggtcgcagc agccaggccg gaaacatcca cgagccgccg cgatcaacgc ggaggcaatt
     2461 gccctggctg tccctggcct tggggtcgct cgagggggtt tcgttcgcgc gcccatccgc
     2521 gggaatgccg gcgtaactgt tgtcgtaaca gtcctccgtc cactgccaga cgttccctgc
     2581 catgtcataa aggccgaatg cgttcggcgg cttcgcgccc accggatgtg gctggccatt
     2641 ctcgcaataa agaccctcga tcttttcgca gccggagttg gcgttgaacc aggcatggac
     2701 aggagcggcc gcgtcgtcat cgccccagta gaacttcgtc gtcgttccgg cgcgtgcggc
     2761 atactcccac tccgcttcgc tgggcagccg ataaggctcg tttgctgcgg ccgcgcccgc
     2821 gccgccgcga tgcgccttgc ggttcaacca ggcgatatag gcacgcgcat cctgccagct
     2881 cacgcacacg accggatcac ggtcagtctg cacatggccg gggctttccc aggtcgactt
     2941 cggatccttc tcccatttga agatggcacg gccgcggccg caaccatcac cggccgggta
     3001 tcccgtctcg cgagcaaatg ccgcgaactc gccacgagtg acgtcgtact tgccgagcgc
     3061 gaacgacggt aatgagacct gatgttgcgg agcttcatca gcaaccgacg ctttattgct
     3121 tccgtgacta gcggcccacg atttttcggc gtcggaagaa cccatggtga aatcgccggc
     3181 gggaacgacg accatttcgg ggcactcact gcaatcgcgg aagacagtgg ccggcttgtg
     3241 cgccgaagtc ttgtggtccg agggctgttt tgtctggtca tacacgtcgc ggatggcgtt
     3301 gatgaccgta tccggctcgt cgaactggat atattccgag ctattgcggg cgaagatctg
     3361 tctggagttc gaagagagtt cgagccagcg cgcctgggcc agcgtggtct cctgctcgta
     3421 tttaaggtgt tccggcgtgt ccggcggctt cctctccaaa tggccgactc cgtggtttcc
     3481 ggatgtaagc acgcggatgg ggcgggagcc gaaggaacgg cggtgtcgtt gcagatagat
     3541 ctcgtccgcc ggagtttgtt ccatctccga ggcataagcg tcatacatgg caaccttggt
     3601 ttgcgcgagg tccagtagtt tggcgttcaa ttccggagac cacgccgcgt cgggcagacc
     3661 tcggaaaaag aaccgctgcg cgcaactctg tggcggccgg tcaggacgcg acgacacctt
     3721 cggcaacggt ttgtgttcgg cgatcaagtc tcggcatgcg cgcaggtcca cagggatgtg
     3781 cgcttgtcct cgatgcgact cttcctgcat cgccttcggt tcgaggtcga cgggatccgc
     3841 gtcatccagg accagccccg caacctcgtc cataaacaga tccgcgaagg tgcgcacgtt
     3901 gtcgccgcca aacgcgctcc cgaccagaat gtacggcccg ccgatgccgg cacgatgcag
     3961 cgcagttcgc agctcctttg cgatgcgcac actggtacgc ggcatcggac cgggttcgct
     4021 gaacccggtg ccggcgcggt catagctgca ggcacgcgtc catttggcga tctccggctg
     4081 caccgtcgac cacgcgggcg cccagtctcc ccatccggaa tcgaagacga ccgtgggcga
     4141 accgctcccc atgcagtaga gattcagacg gaatccgttg acggaaacga gtttaccggg
     4201 ccgcgcgtaa atgatgtccg ccggagagct gccgctgctc gcgccttgtt ggccatagat
     4261 ggcagtgctg gaaaggagca ttgccaggag atagacagtt cttaattgcc tcatcgtcgc
     4321 ctcctacagg tgggagccca ctgactgaat gggacgttcg ccgcaatccg gaggttagtc
     4381 aagccacgcg cgacagcaac ttggaaaacc cgcgaaaaca tcaggaaaca cgtggggtca
     4441 ggccttcggt tattggttgc tcgtaaccac atggcgcata actacttacc cgatcccaaa
     4501 gcggtgttca tgacgagctt cgaagcagca cctctgatcg aacgcgaaac agattttctc
     4561 ggataccgga atcatcgtca cttcgaaaaa ccggaggcga accggtgacc ttcattcgat
     4621 cggacggtgt cgaggaggta tcatttccgt gtatgggact cttttatgtc ggctgtaaag
     4681 tcgtcaacac tcggaattca aagtctgccg ttgttccgag aatgatggtc gacaccggcc
     4741 cggaagcgac ctggatcgat agtgcagtat tggcgaagat cggaatcgaa ccgcgcaaga
     4801 aagacctgca gtttcagatg gcgaatggtt ccatcattac ccgctcggtt ggttacgccg
     4861 tgctcaaagt ggacgaatca gagactgtgg acgaagttgt gtttgcgcaa aagggcgatc
     4921 tccaactgct gggcgcacgc gcgttggaag gcctgaatct aaaggtcgat tcgcgccgca
     4981 agaaactcgt cgcggcgggc ccaatcgttg tcgcggctgc cgtgccgccc caattcggag
     5041 agttgattcg ggtcccgatt catgaacaaa agccccgcca caaggggga
//
LOCUS       DATLVW010000619         4363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_152911, whole genome shotgun sequence.
ACCESSION   DATLVW010000619 DATLVW010000000
VERSION     DATLVW010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152911"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1023)
                     /locus_tag="VNN25_18300"
     CDS             complement(46..1023)
                     /locus_tag="VNN25_18300"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXH93539.1"
                     /translation="MQNDDESQYRGWRRPGPVPPKGIEPEPGESLDYLCGHFRIFQYE
                     KGHRYSTDDAMTAWYATQWAPRVDRAADLGSGIGSVAILSAWRLPGATFCTVEAQEMS
                     LRLARKSIQYNGLEGRFTTYLGDLRDETLLANEAPFDLVTGSPPYWPATDATVAAHPQ
                     AVPARIEVRGTVADYAAAASRILAPGGTFVFVFANKRNQRKGVRSKVESDPPLLATSP
                     TTNVGSLSTLDLTPRQPGEDAGVAFAEEALRSNGLVLLRRRDIIFKEGNPPMISLFAA
                     MRATDLPPGRKAWIEPPLIIRTRTGAVHPEYAAIRISFGFPPGDTAGQV"
     gene            1146..2678
                     /locus_tag="VNN25_18305"
     CDS             1146..2678
                     /locus_tag="VNN25_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH93540.1"
                     /translation="MKEAARRLSTSIFLLLTITATLHAQDLASFEKRTTVRKLDNGLT
                     VIVMERPEAPVFSFTTIVNTGSAQEVPGITGLAHMFEHMAFKGTDKVGTSNYAAESAA
                     LAKVEEAYAAYDHARRDPVNHDEAKIAALDKAWKAAIDDAQKFVVPNEFSKIVDRAGA
                     VGVNAFTNDDETTYFYSMPSNRVELWAYLDSERFLHPVFREFYKERDVVHEERRMRTE
                     STPIGRMIEQALAAAFTAHPYGQPVVGWPSDLESFSATDAKAFFAKYYVPANMVVAVV
                     GDVKASEVMPIMDKYFGRLPKAPAPDPLRTIEPKQTAERSVILHEQAQPVYFEAYHRP
                     AATDPDSATLDAIEMLLSEGRTSRLYRSLVRDKKLAAVAAGFNGFPGRKYPNLFAFFG
                     IPTAGHTAAELAEPIHAEIERLKTEDVSADDLQSIKTRTKAGLLMQLDSNSGLAIQLA
                     TYQTVFGDWRELFREVDKIDKVTAADIKRVANATFTPTNRTTAMIESVKKATPASKGD
                     TK"
     gene            2675..>4363
                     /locus_tag="VNN25_18310"
     CDS             2675..>4363
                     /locus_tag="VNN25_18310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH93541.1"
                     /translation="MKSLRIHAIAAATALLSLPLMAQVSDYHEIKTPPLHQIQMPQPK
                     RVQLANGMVIFLMEDHELPLIRGTARIRGGARDLAADKTGLAGIYSAAWRTGGTTSKT
                     GDELDDFLEARAAAVETNANEDASSVRFHMLKGDFDAVFPIVVDVLEHPAFRQEKVDL
                     AKTSATTAISRRNDDAKGIADREMSKLGFGADSPYARVPEYTTVNGITRDDLVAFHKR
                     FVYPNNIILSIVGDFDSAAMEKKLRDAFGSWAKGPQAPTAAPTGGSPAKAGVYFVAKD
                     DVTQSNIYVVHGGTGVLRNSPDFYAMQVMNEILSGGFSGRLMNDIRTQRGLAYGVGGG
                     VDTTWDRPGLFHIWMGTKSGSTVEAVNALRTDLGDLQTKPFTSDELAQAKEAILNAYV
                     FTADSKSKVLDQRVNLEFYGYPADFYQTYPAKLQAVTADDVARVARKYVQPNQVAVLV
                     VGKEKDFDKPLSTLGAVTPVDITIPEPGAKPVVATAGATAAAPSKPTSSSPEGLALIK
                     KVQSFVGGKAKIDAVVATHSIGSMQAQGPQGPMDVEIESTVKYPDYSHRTVKTPM"
BASE COUNT          887 a         1384 c         1355 g          737 t
ORIGIN      
        1 ggacgatcgt cgcaaaagcc atgagcccat tcggcacttt agactttaga cctgacccgc
       61 agtatccccc ggtgggaagc cgaagctgat gcggatggcg gcgtactccg ggtgtacggc
      121 gccggtgcgg gtgcggatga tgagcggtgg ttcgatccac gctttgcggc cgggcgggag
      181 gtcggtggcg cgcatggcgg cgaagaggga gatcatcgga gggttgcctt ctttgaagat
      241 gatgtcgcgg cggcggagca gcacgaggcc gttggagcgc aaggcctcct cggcgaaggc
      301 gacaccggcg tcttctccgg gctggcgtgg ggtcaggtct aaagttgaaa gtgacccgac
      361 attagtggtg ggagaagtcg ccaaaagcgg tgggtcactt tcaactttag acctgacccc
      421 tttccgttga ttgcgtttgt tcgcgaagac gaagacgaac gtgccccctg gggcaaggat
      481 gcgagacgcg gcggcggcgt agtcggcgac cgttccgcgc acttcgatcc gcgcaggcac
      541 tgcttgcgga tgcgcggcga cagtcgcatc agttgcaggc cagtacgggg gcgagccggt
      601 gacgaggtcg aaaggcgcct cgttggcgag gagcgtttcg tcgcgaaggt cgccgaggta
      661 ggtggtgaaa cgcccttcga ggccgttgta ctggatgctc ttgcgcgcca ggcgaagtga
      721 catctcctgc gcttccactg tgcagaatgt agcaccgggc agtcgccagg ctgagaggat
      781 cgccacggag ccgatgccgg agccgagatc ggcggcgcga tcgacccggg gagcccattg
      841 cgtcgcgtac caggcggtca tggcatcatc ggttgagtag cgatgcccct tctcgtactg
      901 gaagatgcga aaatgaccgc agagatagtc gagcgactcc cccggctcag gctcaatgcc
      961 cttcggggga accggacccg gccgtcgcca gccccgatac tgagattcgt cgtcgttttg
     1021 caataaaaga aacccttctt cgcgtcagac gtatcacgaa aacggctcga tcctcgcgtc
     1081 ccgggacagg tttgtccggt cacgcgcggc aaaatcaaca accatcaaac cactggaggc
     1141 atcatttgaa ggaagcagct cgaagactgt ccacatcaat cttcctgctt ctgacgatca
     1201 cagcgaccct gcacgcgcag gatctcgcct cgttcgagaa gcgcaccacc gtgcgcaagc
     1261 tcgacaacgg cctgaccgtg atcgtcatgg agcgccccga ggcaccggtc ttttcgttca
     1321 cgacgatcgt gaacaccgga tcggcacagg aagtcccggg gatcaccggc cttgcgcaca
     1381 tgtttgagca catggcgttc aaaggcaccg acaaagtcgg gacctcgaac tacgccgcgg
     1441 agtcggcggc gctggcgaag gtcgaagagg cctacgccgc gtacgaccat gcgcgccgcg
     1501 atcccgtcaa ccacgatgag gccaagatcg ccgcgctcga caaagcgtgg aaggccgcca
     1561 tcgacgacgc ccagaagttc gtcgtgccga acgagttcag caagatcgtc gaccgcgccg
     1621 gcgccgtcgg cgtgaacgcg ttcaccaacg acgacgagac gacctacttc tactcgatgc
     1681 cgtcgaaccg cgtcgagctt tgggcctacc tcgactccga gcgtttcctt catcccgtgt
     1741 tccgcgagtt ctacaaagag cgcgacgtcg ttcacgaaga gcgccgcatg cgtaccgaga
     1801 gcacgccgat cggccgcatg atcgagcagg ccctggccgc cgcgttcacg gcgcatccct
     1861 atggacagcc ggtcgtcggc tggccatccg atctggagag tttctcggcc accgacgcca
     1921 aggctttctt tgccaagtac tacgttccgg ccaacatggt cgtggccgtc gtcggcgacg
     1981 tgaaggcgtc cgaggtcatg ccgatcatgg acaaatactt cgggcggctg ccgaaagcgc
     2041 cagcacccga tccgctccgc acgatcgagc cgaaacagac cgcggagcga agcgtcatcc
     2101 ttcatgaaca ggcccagccg gtctacttcg aggcctacca tcgtccggca gcgaccgatc
     2161 ctgattcggc aacgctcgac gccatcgaga tgttgctgtc cgagggacgt acctcgcgct
     2221 tgtatcggtc gcttgtccgc gacaaaaagc tggccgcggt tgccgccgga ttcaacggct
     2281 tcccgggacg gaagtacccg aaccttttcg ccttcttcgg catccccacg gccggtcaca
     2341 cggccgccga gctggcggag ccgatccacg ccgagatcga gcggctcaag acggaagatg
     2401 tttccgccga cgacctgcag tcgatcaaga cgcgcacgaa ggccgggctt ttgatgcagc
     2461 tcgacagcaa ctccggactg gccattcaac tggccaccta ccagaccgtt ttcggcgact
     2521 ggcgcgagct tttccgtgag gtggacaaga tcgacaaggt gacggctgcc gacatcaagc
     2581 gcgtcgccaa cgccacgttc actccgacca accggaccac ggccatgatc gagtccgtga
     2641 agaaagccac tccggcatcg aagggagaca cgaaatgaaa tcgcttcgca tccatgcgat
     2701 cgcggcggcg acagcacttc tgtccctgcc gctgatggct caggtttccg attaccacga
     2761 gatcaagacg ccgccattgc accagatcca gatgccgcaa ccgaagcggg tgcagctcgc
     2821 caacggcatg gtgatttttc tgatggagga tcacgagctt ccgctgatcc gcggcactgc
     2881 ccgcatccgc ggaggcgcgc gtgatcttgc cgccgacaag accggcctcg caggaatcta
     2941 cagcgctgcc tggcggaccg gcggcacgac gtcgaagacc ggcgatgagc tcgacgactt
     3001 cctcgaagcc cgcgccgctg ccgtcgaaac aaacgcaaac gaagacgcct ccagcgttcg
     3061 ctttcacatg ctgaagggcg actttgacgc ggtcttcccg atcgtcgtcg atgtcctcga
     3121 gcatcccgcc ttccgccagg aaaaggtgga tctggcaaag acgtctgcga cgacggcgat
     3181 ctcgcgccgc aacgacgacg cgaaaggcat cgcagaccgc gagatgtcca agctcggctt
     3241 cggcgccgac tcaccgtacg cgcgtgtgcc tgaatacacg accgtcaacg gcatcacccg
     3301 cgacgatctg gtggccttcc acaagcggtt cgtctatccg aacaacatca tcctcagcat
     3361 cgttggcgac ttcgacagcg cagcgatgga gaagaaactt cgcgacgcct tcgggtcgtg
     3421 ggcaaagggt ccgcaggctc cgactgccgc gcccactggc ggctcgccgg ccaaagcggg
     3481 cgtgtacttc gtggccaaag atgacgtgac gcagagcaac atctacgtcg tccacggcgg
     3541 gactggcgtg ctgcgaaaca gccccgattt ctacgccatg caggtgatga acgagatcct
     3601 gagcggaggc ttctccggac gcctcatgaa cgacattcgt acgcagcgcg gcctcgccta
     3661 cggcgtcggc ggcggcgtcg atacgacgtg ggaccggccg ggcctgttcc acatctggat
     3721 gggaacgaag agcggctcga cggtcgaagc ggtcaacgcg ctgcgcacgg atctgggcga
     3781 tcttcaaacg aagccgttca cgtccgatga gctggcgcag gcgaaggaag caatcctcaa
     3841 cgcctatgtc ttcacggccg actcgaagtc gaaggtcctc gatcagcgcg tgaacctcga
     3901 gttctacggc tatcccgccg acttctatca gacctatccg gcgaagctgc aggcggtgac
     3961 ggcggatgac gttgcgcgcg tggccaggaa gtacgtgcag ccgaatcagg tcgcagtcct
     4021 ggtcgtcggc aaggaaaagg atttcgacaa gcctctctcc accctcggcg ccgtaactcc
     4081 ggtcgatatc acgatccccg agcccggcgc caagcccgta gttgcgaccg ccggagcgac
     4141 agccgcggcc ccatcgaagc caacgtccag cagccccgag ggactggcgt tgatcaagaa
     4201 ggtgcagagc ttcgtcggcg gcaaagcgaa gatcgatgcc gtcgtagcaa cgcactccat
     4261 cggctccatg caggcgcagg gtcctcaggg tccgatggac gtggagatcg aaagcaccgt
     4321 gaagtatccc gactacagcc accgcaccgt caagacgccg atg
//
LOCUS       DATLVW010000620         5073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_316352, whole genome shotgun sequence.
ACCESSION   DATLVW010000620 DATLVW010000000
VERSION     DATLVW010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316352"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..555)
                     /locus_tag="VNN25_18315"
     CDS             complement(55..555)
                     /locus_tag="VNN25_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXH93542.1"
                     /translation="MASKVFVGNLDFNTTRDEVEALFAQVGTVRDVFLPTDRESGRPR
                     GFAFVEFESDEDAAKAIEKFNGHDLGGRALRVNAAEDRPPRAGGAGGGRSFGGGGGAG
                     RGGGGGGYSGGGGGGGGYGGGGGGGYGGGGGGGYGGGGGGGAYGGGGGKPKGSRRNIR
                     AKKRGL"
     gene            complement(679..1317)
                     /locus_tag="VNN25_18320"
     CDS             complement(679..1317)
                     /locus_tag="VNN25_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93543.1"
                     /translation="MKSSSRSVVLAVAVVVLTAAEANAAGVDFKDSRRALGREDDIRV
                     DAQIAQDTISSSSPISVTYQIENLTSEPIAVADKICDSSFDADTQTVTLSIGAEVPQG
                     TAMPHLVTIAPGQKRVLHTGAMVQVVQGNSHSPMARVPRYVQITVNVLRDLRPFAKLI
                     EEQSHAATVPLPNNLFDQWVDSVSTVDLNSLPVRWTNSPRAGTAESGAPGRF"
     gene            complement(1346..3142)
                     /gene="lepA"
                     /locus_tag="VNN25_18325"
     CDS             complement(1346..3142)
                     /gene="lepA"
                     /locus_tag="VNN25_18325"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXH93544.1"
                     /translation="MPDPKFIRNFSIIAHIDHGKTTLSDRLLQKTGAVEGREMHEQML
                     DDMDLERERGITIKARAVNLQYRAKDGQDYVFNLIDTPGHVDFSYEVSRSLAACEGAV
                     LVVDATQGVEAQTLANSYLAIANNLDILSVINKVDLPSAEPEKVREQIENVIGLDAHD
                     AVLASAKSGIGVDEILEKIVTKISAPKGDANGPLRALLFDSWYDSYRGVMCLVRIVDG
                     EIRKGMRIKFLSTDRIYEVTEMGVFTPKIKLVDSLGVGEVGFFSGNIKDIVQAKIGDT
                     ITASNASPPMLPGFEEVKPMVFAGIFPINTDDYEALRDAVGKLKLNDASFSYEPESSL
                     ALGFGFRCGFLGLLHMEIIQERLEREFNLELLTSAPGVRYRIQKTDGDELWIENPQKM
                     PDPAYISSIEEPWIEATILTTDEFVGPILALATDRRGVQKKLEYVSANRVMIVYEMPL
                     NEVILDFFDKLKSISRGYASLDYQFVGYRESDLVKMDILINGDPLDALSLIVHKEKAY
                     ARGKLLVEKMKEFIPRQMFAVALQAAIGNRIIARETVKAMRKDVLAKCYGGDISRKRK
                     LLEKQKEGKKRMKRVGKVEIPQEAFLAVLKID"
     gene            complement(3187..3945)
                     /locus_tag="VNN25_18330"
     CDS             complement(3187..3945)
                     /locus_tag="VNN25_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93545.1"
                     /translation="MGRSALLLLFLAVSATNGAAQYTYIVPVSGFAFGFHSQYFTSVT
                     AFNPNSVPATLRYEAVYPAPGNTNCALPASTTVLPRGLVGLSVACFNLHALLLTSDQQ
                     LRLVEEVNANFISMDAAGNSFGRPELEPVEVATDWIAPERDVMIPLVRILEPTDRANL
                     ILVNPNDFLLTVTLHVERQELGESVDSTLQVEPRSFLMSPVVPIPNPISIGFPQGLEG
                     IHRITLRANGKFQAGVSNTDGWGTIYRPAIPLQP"
     gene            complement(3976..4482)
                     /locus_tag="VNN25_18335"
     CDS             complement(3976..4482)
                     /locus_tag="VNN25_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HXH93546.1"
                     /translation="MKPRAATVSDAPAIARIYNEGIDDRVATFETRHRTAEDVVQWLD
                     GRHPVVVVEDDDGAVIAFAATSTYRPRECYAGIAEFSVYAARAARGRGAGRAAMEALF
                     AAARNAGFWKLVSRVFVENTASRALLRSIGFREVGVYERHGQLDGVWRDVVIVEKLLS
                     PPRAAQTS"
     gene            complement(4484..>5073)
                     /locus_tag="VNN25_18340"
     CDS             complement(4484..>5073)
                     /locus_tag="VNN25_18340"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXH93547.1"
                     /translation="ARIVVKADRSQFTVGVSSRATTVTSALRTNNDKMQRVIAALKAH
                     GAAENEISTSTFSITQPFENGRHVVGEYEVQNSVTVTRSAVASISDLLQIAIDAGANQ
                     AWNVRFFLAEPGPVRDKALAAAYNDARARAQKLAAAAGKSVGDLLTITTEQSPRFPLV
                     GGGDLESMRLAGTAPPLQSGMQSVEASIVAQFELK"
BASE COUNT          970 a         1605 c         1494 g         1004 t
ORIGIN      
        1 caaacgtaca aatgaaaagg cctcccgtga gggaggccag gttgtcgtga tcgatcagag
       61 gccgcgtttc ttcgcgcgga tgttgcgccg gctgcctttc ggcttgccgc cgccgccacc
      121 gtacgctccg ccgccgccac caccgccgta gcccccacca ccgccaccgc cgtagccgcc
      181 gcctccaccg ccgccgtaac cgccaccgcc gccgccacca ccgctgtagc cgccaccgcc
      241 accaccgcgg cctgcgccac cgccgccgcc gaatgaacgg ccgccgccgg cgccgccagc
      301 acgaggcggg cgatcctcgg cggcatttac gcgaagcgcg cggccaccga ggtcatgtcc
      361 attgaacttt tcaatcgcct tcgccgcatc ctcatcagac tcgaactcga cgaacgcaaa
      421 cccgcggggt cgacccgact cgcgatcggt cggaagaaac acgtctcgta ccgttccgac
      481 ctgcgcaaac aacgcttcga cctcgtcgcg cgtcgtgttg aagtcgagat tccctacgaa
      541 aaccttggaa gccatcaaca tctcctcttc tgcggctcat gcctgccatc gatggcttcc
      601 ggctttccgc gcgccgaagt gtatcgcgtt gcacgatatg tgcgtgggaa aactcggatc
      661 taccgcccgc cgcctcgctt agaagcgccc gggcgcgccc gactccgcag tgcccgctcg
      721 tggcgaattc gtccagcgca ccggcagaga attgagatcg accgtcgaga cactatccac
      781 ccactgatcg aaaagattgt tcggcaatgg cacggtcgcg gcgtgcgact gctcttcgat
      841 cagcttcgcg aacggcctca gatcgcgaag tacgttgacc gtaatctgca catatcgcgg
      901 cacccgagcc atcggcgagt gcgagtttcc ctggacgacc tgaaccattg cgccggtgtg
      961 cagcacgcgc ttctggcccg gcgcgatcgt taccagatgc ggcatggccg ttccctgcgg
     1021 aacttccgcg ccgatcgaga gcgtgaccgt ctgcgtgtcc gcatcaaacg acgaatcaca
     1081 gatcttgtcc gcaacggcga tcggctcgga ggtcagattc tcgatctggt acgtgacgct
     1141 gatcggtgac gaggaggaga tcgtgtcctg ggcgatctgg gcatcgacgc gaatgtcatc
     1201 ttcgcgtccg agcgctcttc gcgagtcctt gaaatcgacc ccggcggcat tcgcctccgc
     1261 ggcggtgaga acgacgactg caacggcaag gactaccgaa cgacttgaag atttcatcgc
     1321 acacctcccc ggataaggag gtgactcagt caatcttcaa cactgccaga aaggcctcct
     1381 gcggtatctc gactttcccc acccgtttca tccgtttctt cccctctttc tgcttctcga
     1441 ggagcttgcg tttgcgcgag atgtcgccgc cataacactt ggcgaggacg tccttgcgca
     1501 tcgctttcac ggtttcgcgg gcgatgattc gattaccgat ggctgcctgc aaagcgacgg
     1561 cgaacatctg gcgggggatg aactccttca tcttctcgac gaggagcttg ccgcgcgcgt
     1621 atgccttctc cttgtgcacg atgagagaga gagcatcgag cgggtccccg ttgatcagaa
     1681 tgtccatctt caccagatcg ctttcccggt aacccacgaa ctggtaatcg agcgaggcgt
     1741 atccgcgtga gatcgatttc agcttgtcga agaaatcgag gatcacctca ttcaacggca
     1801 tctcatacac gatcatgact ctgttggcgc ttacgtactc gagcttcttc tgtacgcctc
     1861 gccgatccgt cgcaagagcg agaatcgggc caacgaattc atcggtcgtg aggatggtcg
     1921 cttcgatcca cggttcctca atcgagctga tgtacgcagg gtcaggcatc ttctgcggat
     1981 tctcgatcca cagctcgtcg ccgtcggtct tctggatgcg atagcgcacg ccgggcgcgg
     2041 aggtgagcag ctccagattg aactcgcgct ccagccgctc ctgaatgatc tccatgtgca
     2101 gcagaccgag aaaaccgcag cggaaaccga agccgagtgc gagcgagctc tcgggctcgt
     2161 agctgaacga ggcatcgttg agcttgagct tcccgacggc gtcgcgcagg gcttcgtagt
     2221 cgtcggtgtt gatcggaaag atgccggcga agaccatcgg cttcacttcc tcgaagccgg
     2281 gaagcatcgg cggcgatgcg ttcgatgccg tgatcgtgtc gccgatcttg gcctggacta
     2341 tgtccttgat gttgccggag aagaagccga cttcgccgac gccgaggctg tcgacgagct
     2401 tgatcttcgg cgtgaagacg cccatctcgg tcacttcgta aatgcggtcg gtcgaaagga
     2461 acttgatgcg catccctttg cggatctcgc cgtcgacgat gcgcaccagg cacatgaccc
     2521 cgcggtacga gtcgtaccag gagtcgaaca gcagcgcgcg cagcggaccg ttcgcgtcac
     2581 ccttcggcgc gctgatcttc gtgacgatct tctcgaggat ctcgtcgacg ccgatgcccg
     2641 acttcgcgga ggcaagaacg gcgtcgtgag catcgaggcc gatgacgttc tcgatctgct
     2701 cgcgcacctt ctccggctct gccgacggca agtcgacctt attgatgacc gagaggatgt
     2761 cgagattgtt ggcgattgca aggtagctgt tcgccagcgt ctgcgcctcg actccttgag
     2821 tggcgtcaac cacgagaacc gcaccttcgc acgcggccag actgcgcgag acctcgtacg
     2881 aaaagtcgac gtgtccgggc gtgtcgatca ggttgaagac gtagtcctga ccatccttgg
     2941 cgcggtactg caggttgacc gcgcgcgctt tgatggtgat gccacgctcg cgctcgaggt
     3001 ccatgtcgtc gagcatctgc tcgtgcattt cacggccttc gacagcgccc gtcttctgca
     3061 gcagacggtc cgagagcgtc gtcttgccgt ggtcgatgtg agcgatgatc gagaaattac
     3121 ggatgaattt ggggtctggc attgcgcggc gtgcggtcta acacgcagat ccgcgtttgt
     3181 catccctcag ggctgcaagg ggatagcggg cctgtagatt gtcccccagc catcggtatt
     3241 cgaaacaccc gcctggaact ttccgttcgc tcggagcgtg attcgatgaa tgccttcaag
     3301 accctgcgga aatccgattg atatcgggtt gggaatcggc accacgggag acatcagaaa
     3361 tgaccgcggt tcaacctgca gagtgctatc caccgactcg ccaagctcct gccgctcgac
     3421 atggagcgtg acggtaagta gaaagtcgtt cggatttaca aggatgaggt ttgctctgtc
     3481 cgttggttcg agaatcctga ctagaggaat catcacgtca cgctcaggcg caatccagtc
     3541 ggtagccact tcgaccggtt ccagttcggg gcgcccgaat gagtttccag ccgcatccat
     3601 ggaaatgaaa ttggcattga cctcttcgac gaggcgtagc tgctgatcgg aagtaagaag
     3661 aagcgcgtga agattgaaac aggcaacgga caaaccaacc aggcctcgag gcaaaacggt
     3721 cgtggaggcc ggcaatgcgc agttcgtgtt tcctggagcc gggtagaccg cttcgtatcg
     3781 cagcgtggca gggacagagt tgggattgaa tgccgtgacg ctcgtaaagt attgactgtg
     3841 aaacccgaac gcgaaccccg ataccggcac gatgtacgtg tattgcgcag ctccattcgt
     3901 tgcggaaacg gcgagaaaaa gcagcaacag tgcagagcgg cccatcgact gacacgacct
     3961 gcgatgacga tgccactacg atgtttgagc tgccctgggc ggcgaaagca acttctccac
     4021 gatcaccaca tcacgccaca ctccatcgag ctgcccgtgg cgttcgtaaa cgcccacttc
     4081 gcggaatccg attgatcgga gaagcgcacg gctggccgtg ttctcgacaa agactcgcga
     4141 aaccagcttc cagaaaccgg cattgcgcgc agcagcgaaa agcgcttcca tcgccgcacg
     4201 cccggcacct cgtccgcgcg cggcccgcgc cgcatagacg gagaactccg cgattccggc
     4261 gtagcactcg cgtggccggt aggtcgacgt cgcggcgaac gcgatcaccg cgccgtcatc
     4321 gtcttcaacg acgacgactg ggtggcgtcc gtccagccac tgcaccacgt cctcggcggt
     4381 gcggtggcgt gtttcgaatg tcgcgacccg gtcgtcgatc ccttcgttgt aaatgcgcgc
     4441 gatcgctggg gcgtcggaca ccgttgccgc gcgcggcttc atctcacttc agctcgaact
     4501 gcgccacgat gctcgcctcg accgactgca tacccgactg cagcggcgga gcggtaccgg
     4561 cgagcctcat cgactccagg tctcctccgc cgaccagcgg aaaccgcggc gactgttcgg
     4621 tggtgatcgt cagcagatcg ccgaccgact ttccagccgc agcggcaagc ttctgcgcgc
     4681 gggctcgggc atcgttgtac gccgccgcga gtgccttgtc gcgcacaggg cccggttcgg
     4741 ccaggaagaa tcgtacgttc cacgcctggt ttgcgccggc gtcgattgcg atctggagca
     4801 gatcgctgat gctcgccaca gcgctacgcg tcaccgtgac ggaattctga acttcgtatt
     4861 cgccgaccac gtggcgtcca ttttcaaaag gctgcgtgat cgaaaaggtc gacgtgctga
     4921 tctcattctc cgctgcgccg tgtgccttca acgcggcgat gacccgctgc atcttgtcgt
     4981 tgttggtgcg cagggcgctc gtcacggtgg ttgcccgcga cgagaccccg accgtgaact
     5041 ggcttcgatc ggctttcacg acgatgcggg cac
//
LOCUS       DATLVW010000621         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_604932, whole genome shotgun sequence.
ACCESSION   DATLVW010000621 DATLVW010000000
VERSION     DATLVW010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604932"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VNN25_18345"
     CDS             <1..1341
                     /locus_tag="VNN25_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXH93548.1"
                     /translation="HGTFRDVEAQLPRIARMGFDVLYLPPIHPIGTTFRKGPNNTVST
                     ETSDPGSPWAIGAKDGGHKAIHPDLGTLDDFRRLAEAARARDIELAMDIAFQGSPDHP
                     YVAEHDDWFQKRPDGTIQYAENPPKKYQDIYPFHFESDAWADLWKELVGVFRYWIDNG
                     IRVFRVDNPHTKPLPFWHWVIRTVKKHHPDVIFLAEAFTRPKIMYWLAKAGFSQSYTY
                     FAWRNTKYELTEYFGQITKPPVSDFFRPNAWPNTPDILPEYLQYGGRPAFVIRLMLAA
                     TLSSNYGIYGPAFERSIGEARDPGGEEYRDSEKYEIKHWPQVDDDLTELIAAVNRIRR
                     QNPSLQQNATLKFHETTNDDIICYSKSAGDESVLMIINVDPHNTQAGYVELDLSVLNL
                     DANRPFQVHELLSGARYTWHGPRNYVKLDPNVIPAHIFRIRRKVRSEKDFEYFL"
     gene            1485..>2787
                     /gene="treS"
                     /locus_tag="VNN25_18350"
     CDS             1485..>2787
                     /gene="treS"
                     /locus_tag="VNN25_18350"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HXH93549.1"
                     /translation="MNDLWYKDGIIYQTHVRAFFDSNNDGIGDFQGLTRKLDYLQDLG
                     ISIIWLLPFYPSPLRDDGYDIANYNAVHPSYGTLSDFKGFLKEAHRRGLKVITELVIN
                     HTSDQHEWFQKSRRAKPGSPWRDFYVWSDTPAKYKDARIIFKDYETSNWTWDPVANAY
                     YWHRFFSHQPDLNFDNPAVHAALLKAFDFWMEMGVDAFRLDAIPYLYEREGTTCENLP
                     ETHAYLKSLRAHVDAKHPGKMLLAEANQWPEDSLPYFGDGDECHMAFNFPVMPRMYMA
                     LAQEDRFPIIDIMAQTPSIPENCQWAMFLRNHDELTLEMVTEEDRDYMWRTYASDRQA
                     RINLGIRRRLAPLMQNHRLRIHLLNGLLFSLPGTPIIYYGDEIGMGDNIYLGDRNGVR
                     TPMQWSPDRNAGFSKANPQQLYMPAIIDPQYHYEQVNVEQQQ"
BASE COUNT          614 a          869 c          759 g          545 t
ORIGIN      
        1 cacggaacgt ttcgcgatgt cgaggcgcag ttgccgcgca tcgctcgtat gggcttcgac
       61 gttctctacc tgccgccgat ccacccgatc gggacgactt ttcgcaaggg gccgaacaac
      121 acagtcagta cggaaacgag cgaccccggc agcccctggg cgatcggtgc gaaggacggc
      181 ggccacaagg caatccaccc cgacctcggc acgctcgacg attttcggcg gctggccgaa
      241 gcggcgcgcg cgcgtgacat cgaactggcg atggatatcg cctttcaggg ttcgccggat
      301 catccctatg tggccgagca cgacgattgg tttcagaagc ggccggacgg cacgattcag
      361 tatgccgaga atcctcccaa gaaatatcag gacatctacc cgttccactt cgagagcgac
      421 gcctgggccg atctttggaa ggagctggtc ggcgttttcc gctactggat cgacaatggg
      481 atccgtgtct tccgcgtcga caatccgcac acgaagccac tgccgttctg gcattgggtc
      541 attcgtacgg tcaagaagca ccatcccgat gtgatcttcc tggccgaggc gtttacgagg
      601 ccaaagatca tgtactggct ggcgaaggcc ggcttctcgc agtcgtatac gtactttgcg
      661 tggcgcaaca cgaagtacga actgactgaa tacttcggac agatcacaaa gccgccggtc
      721 agcgacttct ttcggccgaa cgcctggccg aatactccgg atattctccc tgagtatctc
      781 caatacggtg gacgcccggc atttgtcatc cggctgatgc ttgccgccac tctttcctcg
      841 aactacggca tttacggccc cgcgttcgag cgttccatcg gcgaggcccg cgatcccggc
      901 ggcgaggagt atcgcgactc ggagaagtac gagatcaagc actggccgca ggtggacgat
      961 gacttgacgg agctgatcgc cgcagtcaat cgcatccggc gccagaatcc gtcccttcag
     1021 cagaacgcga cgttgaagtt tcacgaaacg acgaacgacg acatcatctg ctacagcaaa
     1081 tcggcgggcg acgagtcggt cttgatgatc atcaacgtcg atccgcacaa cacgcaagcc
     1141 ggctacgtcg agctcgatct ctccgtgctg aacctcgacg ccaatcgccc attccaggtc
     1201 cacgagctgc tttcgggtgc gcgctacaca tggcacggac cgcgaaacta cgtcaagctt
     1261 gatccgaatg taatccccgc gcacattttc cggatacgtc gcaaggtcag atcagaaaag
     1321 gatttcgagt atttcttatg aatccgaacc cgtgtcatcc tgagccgcca acggacggcg
     1381 aaggatctca aaatacgatg cacaagccac atgccgtaca ttttgaggtc cttcgccgtc
     1441 cgtttggcgg ctcaggatga caccgcgggt aagcgaagca attaatgaac gacctctggt
     1501 acaaagatgg gatcatttat cagacgcacg tccgtgcgtt cttcgattcc aacaacgacg
     1561 gcatcggcga ttttcaggga ctgacgcgca agctcgacta tctccaggac ctgggaatca
     1621 gcatcatctg gcttctaccg ttctaccctt cgcctctgcg cgacgacggc tacgacatcg
     1681 ccaactacaa cgccgtccat cccagctacg gcacgctgag cgatttcaag ggattcctga
     1741 aagaggcgca ccgccgcggg ctgaaagtga tcacagagtt ggtcatcaat cacaccagcg
     1801 accagcacga gtggttccag aaatcacgcc gcgccaagcc gggctcgcca tggcgcgact
     1861 tctacgtctg gagcgatacg cctgcgaagt acaaggacgc acgcatcatc ttcaaagact
     1921 acgaaacgtc gaactggacc tgggacccgg tcgccaacgc ctattactgg caccgcttct
     1981 tctcgcacca gcctgatctt aacttcgaca atcccgcggt tcacgcagcg ctcctcaaag
     2041 cattcgactt ctggatggag atgggcgtgg acgccttccg gctcgacgca attccctatc
     2101 tgtacgagcg cgagggaacg acttgcgaga acctgccgga gacgcatgcc tacctcaagt
     2161 cgctgcgtgc gcacgtcgat gccaaacacc cgggcaagat gctgctggcc gaggccaatc
     2221 agtggcccga ggactcgctg ccgtattttg gagatggcga cgagtgccac atggcgttca
     2281 actttccagt tatgccgcgc atgtatatgg cgctcgcgca ggaggaccgc tttccgatca
     2341 tcgacatcat ggcgcagacg ccgtccattc ccgagaattg ccagtgggcg atgtttctgc
     2401 gcaatcacga tgaattaacg ctcgaaatgg tcaccgaaga agaccgcgat tacatgtggc
     2461 gtacctacgc ctccgatcgg caggcccgca tcaacctcgg catccggcgc cgcctcgcgc
     2521 cgttgatgca gaatcatcgc ctgcgcattc acttgttgaa cggactgctc ttctcactgc
     2581 ccggcacgcc gatcatctat tacggcgacg agatcggaat gggcgacaac atctacctcg
     2641 gtgaccgcaa cggcgtccgc actccgatgc agtggagtcc cgaccgcaat gccggtttct
     2701 cgaaagcgaa tccgcagcag ctctacatgc cggccatcat cgatccgcag tatcactatg
     2761 agcaggtcaa cgtcgagcag cagcagt
//
LOCUS       DATLVW010000622         7990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_523260, whole genome shotgun sequence.
ACCESSION   DATLVW010000622 DATLVW010000000
VERSION     DATLVW010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523260"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VNN25_18355"
     CDS             <1..1136
                     /locus_tag="VNN25_18355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH93550.1"
                     /translation="QGCPPAQPWVETVTFGSLSSQLRAEMNASFDQWERLKVVAGAEM
                     AKSSIQSQVDQKASGPGILFHSVATSPEQIEHTDVALYAQGSWKPRPWVKLVFAGRVS
                     HNEINNKPGAAGYGTLFTPRAGVILSPTGKHIVLKAIYSEAFKDPTDAEKFGVLAGVN
                     EYPSFGLKPERVRNGELSAAWEPSERFSADASLYRASYSNVVGFDPVGGCFGAGCLRY
                     ANRDRIVVTGLQTDVRYHAGATEWWGNYTHTDPRLTNPDDARFDSLPVADIAGNRLNA
                     GIDVRLRDRWNAGLRAHYVGERRTGQGTTDAANPFTRMPPYTTADAAVSDRRLIPRAT
                     LQLGVDNLFDKQYYDPATDPLAGAPRVLQAGRTVYLRLIVTLR"
     gene            complement(1133..1705)
                     /locus_tag="VNN25_18360"
     CDS             complement(1133..1705)
                     /locus_tag="VNN25_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93551.1"
                     /translation="MSEETNADVQNSSNSGIGCIHDSKTQGVINRSVSQGENSTVPFT
                     TDTIWWGANLTNFTGCVQVTAPGGNPLGNSQLMTVTSDTAPEPSTPPDPSAPMALIVN
                     NAGSGVQWAYSDNTGSFAGPLDDDGAFQAVPYSQQTIWYGVYLSGFTGYIEVVGPGGS
                     VNQGYKSQINVEYPDQTDDDKRAHKNQGKH"
     gene            complement(1977..2669)
                     /locus_tag="VNN25_18365"
     CDS             complement(1977..2669)
                     /locus_tag="VNN25_18365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH93552.1"
                     /translation="MSERLALGSRIDPLLDRLYALNTSQDEALGSYFSARAQEESFDW
                     NEFDARTNQFLSDKLVALDREKAEFCYHLCRALRARRVVEAGTSFGVSTLFLAAAVRD
                     NVRDDGGSGVVIATEYEPAKAAAARNHFAEAGLSEWIELREGDLRKTLVDVGGPVDFM
                     LVDIWTPMARPALELVAPALRNGAIVICDNTAQFRDAYRDYFGFVNDPANRLRTMTLP
                     FEGGLEFTVRED"
     gene            complement(2784..7946)
                     /locus_tag="VNN25_18370"
     CDS             complement(2784..7946)
                     /locus_tag="VNN25_18370"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXH93553.1"
                     /translation="MKPPITACPSFGSASTNYIGAVPIAIVTADFNRDGKADVAMAAS
                     GNQIEVMLGSGSGGFLSSSYYSVGTAPVSITTADFNGDGKLDLAVANAISNNVSILLG
                     NGDGTFQTAVVYAAGSNPFSVTVADFNGDGKADLVVANNGGNTVSILMGQGDGTFLAA
                     VSYAAGSGPFWVSAADLNGDGKSDLVVADYNSNKLAVLLGNGDGTMQAAVFYASGTGP
                     SSIAIGDFNGDGKADLAASNDGSNNVSILAGVGDGTFQAAVNYAVPSGPHVISIIDVN
                     GDGSPDLAVANNAASSVSILTSRGDGTFQAAVNYPATGAAQIAVLDLNGDGRSDLAVI
                     DSNDYYLFTLLNNGVCALNCNTIAPAVSYAAGTTPKSVAAGDFNGDGKLDVVAANGGA
                     NNVSLELGAGDGTFLPSGTYNAGNAPNSVDTGDFNGDGKLDLAVANSGSNDVSILLGN
                     GNGTFQSAVAYGAGSSPHGVAVADFNRDGKADLVVANNGSSNVSILLGSGTGTFLTAV
                     NYGAGTNPESVAVGDFNRDGKLDLAVANSGSNNVSILLGNGDGTFQTATTVSVGTSPF
                     SIIAADLSGDGKLDLAVANSGSNNISVLIGNGSGAFAAAVNYAVGTNPRGVAAGDLNN
                     DGKLDLAAANYGSNNVSILIGNGNGTFGAASNSGAGTSPSALVLRDFNRDGKPDLATA
                     NSGSSDISILLNTCPVPDLTITKTHTGSFTQGDTGKGYTITVSNVGNASTDGSTVTVI
                     DTIPAGLSATALSGTGWTCVLPTVSCNRSDTLTAGASYPTISLTVNVANSAPATVTNK
                     VTVSGGGELNAVNSSASDAATVAPLTDLVIASTHLGNFTQGDSGKSYRITVRNAGGLP
                     TSGTITAIDTLPTGLTATAISGNGWACNLPSLTCTRSNVLAASSSFPAITITVNVAVN
                     APSSVSNVATVSGGGESNAGNDTASDPTTIWSSQTCGSFGAGVGYPVGSYPSSIATGD
                     FNHDGKTDLAVTSYYSASVSILLGDGAGNFAAAVNYSVGSYPRSIATGDFNNDGVVDL
                     VVANVDSGSMSILIGNGDGTFAPAVSYSDSDTPEWVTVGDFNGDGNADVAVVNYYSTV
                     SIWLGNGNGTFQSKVNYSTPVYGATFIVSADFNGDGALDLGIGGTYNGSFGVLLGNGD
                     GTFQTTINIPVNYYSSFAAVGDFNGDGKPDIVLSTYYSSYVSILLGNGNGTFQPVVQI
                     PAPYGNNYLAVDDLNGDGKADIAISGSSVSVLLGNGNGTFQTAISFPSIGGNGPLAIG
                     DFNGDGKADFAVGSGYEYKVYVLLGGCPDLTIAKSHSGNFSGGQSNATYSLVVTNSGG
                     GSTGALVTVTDTLPTGMTATAMSGSGWSCDLPNVRCTRSDSLAGGASYPTITLTVSVA
                     KTTATSVINTATVSGGSETNTANDTASDPTTVVQVADLTITKTHDGSFASGQSGRVYT
                     ITVTNNGGAATSGTVTVIDTVPVGLTPTAIAGGGWSCDLPSRTCTRSDALAIGASYPA
                     ITLTVNVDNNAPSNVTNTATVSGGGEVLTSNDTASDLTKIITTPTNLAATAIATTQVT
                     VSWDPVVNATNYQILRSANHGAFTVIGAPLSNNFVDTSLQPNTTYLYQVRAADASTLG
                     SPSSVDLATTILFTDDPVSGGSTFIKAVHIMELRTAVNAVRAAAGLTPVTFTDASLAG
                     AATKALHVTELRSALDAARAAIGVAPMIYTDPTLAGNRIKSAHIREVRAGVK"
BASE COUNT         1490 a         2350 c         2514 g         1636 t
ORIGIN      
        1 ctcagggctg cccgccggcg cagccgtggg tggagacggt taccttcgga agtctttcca
       61 gccagcttcg cgcagagatg aacgcctcgt tcgatcaatg ggagaggttg aaagtggtcg
      121 ccggagccga gatggcgaag agctcgatcc agagtcaggt ggatcagaag gccagcgggc
      181 cggggatcct gtttcactcc gtcgctactt cgccggagca gatcgagcac accgacgtgg
      241 cgctgtacgc acagggctcg tggaaaccgc ggccatgggt caagctcgtc ttcgctggcc
      301 gcgtcagcca caacgagatc aacaacaagc cgggcgctgc cggctacggt acgctcttca
      361 caccgcgcgc gggcgttatc ctttcaccca ccggcaaaca cattgtcttg aaagcaatct
      421 attcggaggc gttcaaagat cccaccgacg ccgagaagtt cggcgtgctt gcgggtgtca
      481 atgaatatcc gagctttgga ttgaagccgg agcgtgtgcg caacggagaa ctgagcgcgg
      541 catgggagcc atcggagcgt ttctcggccg acgcgtcgct ctatcgggcg agctactcca
      601 atgtcgtcgg cttcgatccc gtcggcgggt gcttcggcgc aggctgtctc cgctatgcga
      661 atcgcgacag aatcgtcgtc accggactgc agacggacgt tcgctatcac gccggtgcaa
      721 cggagtggtg gggaaactac acgcataccg atccacggct caccaatccg gacgacgcgc
      781 ggttcgattc cttgcccgtc gccgacatcg caggcaatcg cctcaatgcg ggcatcgatg
      841 tacgtttgcg agaccgctgg aacgcgggct tgcgcgcgca ttacgtcggc gagcggagga
      901 ccggccaggg gacgacggac gcggccaatc cgttcacacg gatgccgcct tacacgacgg
      961 ccgatgcggc cgtgagcgac cgccgtctga ttccgcgcgc cacgctgcaa ttgggcgtcg
     1021 acaatctgtt cgacaagcaa tactacgacc cggccacaga tcctctggca ggcgcaccac
     1081 gtgtgctgca ggcggggcgg acggtctact tgcgtctgat cgtcacgctt cgctagtgct
     1141 taccttggtt cttatgtgcc cgtttgtcgt catcggtttg gtcggggtac tcgacgttga
     1201 tctgggattt gtagccctga ttcacgctgc cgccagggcc gaccacctcg atgtaccccg
     1261 tgaagcctga caaatagacg ccgtaccaga tagtctgttg cgagtacggg accgcctgga
     1321 acgcgccgtc gtcgtcgaga ggccccgcga acgagcccgt gttgtctgaa taggcccact
     1381 ggactccact tcccgcgttg ttcacgatga gcgccatcgg cgccgaagga tcgggaggag
     1441 tcgagggctc gggagccgtg tccgaggtga ccgtcatcag ttggctattc cccagcgggt
     1501 tgccgccagg cgctgttacc tggacgcatc ctgtgaagtt cgtgaggttc gctccccacc
     1561 agatcgtgtc ggtcgtgaac ggcacagtac tattctcacc ctgggaaaca gagcggttga
     1621 tcacgccttg cgtcttgctg tcatggatac agccgatccc tgagttcgac gaattctgga
     1681 cgtcggcatt ggtctcttcg gacatggagc tcctcctctc gagcggtgca tacgcacgta
     1741 cggcgttttg atgttttcgg cggggacagc ggccatatat atgctcgcgt tggcaatccg
     1801 tcaacccggc tcctttgcac tttgcagatt ggagagacac gtctgcgatt gcgacaacga
     1861 tcaagcttcg gttgtcgtgg cggtggggag gcaggccgtc gactcatgaa tcggatcgca
     1921 ctaggtcttt gctcccaccc ccgacccccg acggcctacg acccccgcac acgggctcaa
     1981 tcctcgcgaa cggtgaattc gaggccacct tcgaagggga gcgtcatcgt ccgcagtcgg
     2041 ttggcgggat cgttgacgaa gccgaaatag tcgcgatagg catcgcgaaa ctgcgccgtg
     2101 ttgtcgcaga tgacgatcgc accgttgcgc agcgccggcg cgacaagctc cagcgctggc
     2161 cgtgccatcg gcgtccagat gtcgacgagc atgaaatcga ccgggccgcc aacgtcgacg
     2221 agcgtcttgc gcaaatcgcc ttcacgcagt tcgatccact ccgacaaccc agcctcggcg
     2281 aagtgattcc gcgcagcggc ggccttggcc ggctcgtact ccgtggcgat gacgacgccg
     2341 gagccgccat catcgcgcac gttgtcgcgc acggcggcag caagaaacag tgtcgagacg
     2401 ccgaacgaag tccccgcttc gaccacgcgt ctggcgcgca acgcacggca caggtggtag
     2461 cagaactcgg ccttctcgcg atcaagcgca acgagcttgt cgctcagaaa ctgattcgtc
     2521 cgcgcatcaa actcgttcca atcgaacgac tcttcctgcg cccgcgctga gaaatacgat
     2581 cccagcgcct catcctggct cgtgttcagg gcgtaaaggc ggtcgagaag gggatcgatc
     2641 ctggaaccga gtgcgagtcg ttcgctcatg gattcaattt tagtattggc cggttgaaaa
     2701 tcttccagca cagagacact gagatcacag aggtaaagaa ggtaactctg tgacctctgc
     2761 gtctctgtgg tgcatggttc cgatcacttc acccctgccc ggacctcgcg gatgtgggcg
     2821 gatttgatgc ggttaccggc gagggttgga tcggtgtaga tcatgggggc tacgccgatc
     2881 gcggcccgcg cggcatcaag agcggatcgc aattccgtta cgtgcagcgc tttggtcgcc
     2941 gcccctgcca gcgaagcatc cgtaaacgtt actggtgtta atccggcggc cgcgcgtacg
     3001 gcattcacgg cggttcgcaa ctccatgatg tgtacggcct tgatgaaggt cgagccgccg
     3061 gagaccggat cgtcggtgaa gagaatggtc gtggcaaggt cgacgctgct cggtgatccg
     3121 agggtcgagg cgtctgcggc gcgcacctgg tagaggtacg tagtgttcgg ctgcagcgac
     3181 gtgtcgacga agttgttcga cagcggcgcc ccgatgacgg tgaacgcgcc gtggttcgcg
     3241 ctgcgcagga tctgataatt cgtggcgttg acgaccggat cccagcttac ggtcacctgc
     3301 gttgtagcga ttgcggtggc ggccagattc gtgggtgtcg tgatgatctt cgtcagatcg
     3361 ctcgccgtgt cgttggaagt caggacctct ccgccgccgg agaccgtggc ggtattcgta
     3421 acatttgacg gcgcgttgtt gtcgacgttc acggtgagcg taatcgccgg atagcttgcg
     3481 ccgattgcca gcgcatcgct cctcgtacag gtgcgcgagg ggagatcgca gctccagccg
     3541 ccgcccgcga tggcggtagg cgtcaacccg accggaaccg tgtcaatcac tgtaaccgtt
     3601 ccgctggtgg cggctccgcc gttgttcgtc accgtgatcg tgtaaaccct tccgctctgc
     3661 cccgacgcaa aactcccatc gtgagtcttt gtaatggtga gatccgctac ttgcacgacc
     3721 gtcgtcggat cgctggcggt gtcgttggcg gtgttggtct cactaccgcc ggatacggta
     3781 gcggtgttga tcacgctcgt ggccgtcgtc ttggccacgc tcacggtgag ggtgatggtt
     3841 ggataactgg cgccgcccgc cagcgaatcg ctccgggtgc agcgcacatt cgggaggtca
     3901 caggaccatc ccgacccgct catggcggtc gcggtcatcc cggtgggaag cgtgtcggtg
     3961 accgtaacaa gggctcctgt cgagcctccg cccgagttgg tgacgaccag cgagtaggtg
     4021 gcgttgctct gtcctccgga gaagtttcca ctgtggctct tcgcgatcgt gaggtcggga
     4081 caaccgccca gcagcacata gactttgtac tcatagccgc tgcctaccgc aaaatcggct
     4141 ttgccatcac cgttgaagtc gccgatcgcg aggggcccgt tccctccgat ggaaggaaaa
     4201 ctgatcgcag tctggaaggt cccattgccg ttacccagca ggaccgagac gctcgagcca
     4261 gaaatcgcga tatcggcttt cccatctccg ttgagatcgt cgaccgccag gtagttgtta
     4321 ccgtagggag caggtatttg gactaccggt tggaatgtac cgttgccgtt acccaggaga
     4381 atcgacacat aggacgaata gtaggttgac aagacaatgt ctggtttccc gtctccgttg
     4441 aagtctccga cggcggcaaa cgagctgtag tagttcaccg gaatattgat cgtcgtttgg
     4501 aaggtgccat ccccattgcc gagcagaact ccaaacgaac cgttgtaagt gccgccgata
     4561 ccgaggtcga gagcgccgtc tccattgaag tccgcgctga cgatgaatgt cgcgccatag
     4621 accggcgtcg aatagttcac tttcgactgg aacgtgccgt tcccgtttcc gagccatatc
     4681 gagacggtcg aataataatt gacgactgcc acgtcggcgt ttccatcgcc attgaaatcc
     4741 cccaccgtta cccattcggg ggtgtcgcta tcggagtaac tcacggcagg cgcgaatgtt
     4801 ccatctccgt tcccgatgag gatcgacatg ctgccggagt cgacgtttgc cacgacgaga
     4861 tcaacgacgc cgtcattgtt gaaatcgccg gtggcaatgg accgagggta acttcccacg
     4921 gaatagttga cagccgccgc gaaattgccg gcaccatccc ccaggaggat cgagacactc
     4981 gcagagtagt agctcgtgac ggccagatct gtcttcccat catggttgaa gtcgccagtg
     5041 gcaatcgagc ttgggtacga gcctactgga tagccaacgc ccgctccgaa gcttccgcag
     5101 gtctgactgc tccagatcgt ggtcggatcg ctggcggtgt cattgccggc gttactctcg
     5161 ccacctccgg agacagtggc cacgtttgaa acgctgctgg gcgcgttcac ggcgacgttt
     5221 accgtgatcg taatggctgg gaaactactg ctggcggcga gaacgttgct gcgcgtgcag
     5281 gtcaacgagg ggagattgca cgcccagccg ttgccggaga tcgcagttgc ggtcagaccg
     5341 gtagggagcg tgtcaatggc ggtaatcgta cctgacgtgg gcaggccgcc ggcatttcgc
     5401 acggtgatgc gataactctt cccggagtcg ccctgcgtaa aattgccgag gtgcgtactg
     5461 gcgattacga gatcggtcaa gggagcgacg gtcgcggcat cgctggcgct gctattgacc
     5521 gcgttcagct cgccgccgcc cgatacggtc accttgttcg tcacagtggc cggcgcgctg
     5581 ttggcgacgt tcaccgtcaa actgatggtc gggtagctcg ctccggccgt cagagtgtcg
     5641 cttcggttgc acgagacggt cgggagtacg cacgtccacc ccgtaccgct gagcgcggtg
     5701 gccgagaggc cagcgggaat ggtgtcgatg acggttaccg tcgagccatc ggtggaagcg
     5761 tttccgacat tgctcacggt gattgtgtat ccctttcccg tatcgccttg tgtgaagctt
     5821 ccggtgtgtg tcttggtgat ggtgagatcg ggaaccggac acgtattgag cagaatggaa
     5881 atgtcgctcg agccgctgtt cgccgtggcg aggtcgggtt tgccgtcgcg attgaaatcg
     5941 cgaagaacga gcgcactcgg actcgtccct gcgccgctgt tggacgccgc tccgaacgtg
     6001 ccattgccgt tcccgatcag gatcgagacg ttattcgagc cgtagttcgc ggcggccagg
     6061 tcgagtttcc cgtcattgtt gagatcgccc gccgcaacgc cgcgtgggtt cgtgccgacg
     6121 gcgtaattga ccgcagcagc gaacgctccg ctgccgttgc cgatgaggac ggagatgttg
     6181 ttcgatccgc tgttggcaac ggcgagatcg agcttcccat cgccactgag atccgccgca
     6241 atgatcgaga agggactcgt accgacgctt acagtggtcg cggtctggaa tgtcccgtcg
     6301 ccgttcccga ggaggatgga gacgttgttg gatccggagt tcgccacggc cagatcgagc
     6361 tttccgtcgc ggttgaaatc gccgaccgcg accgattcgg gattggtgcc ggcgccatag
     6421 ttcaccgcgg tcaggaaggt gcccgttccg ctgccaagaa ggatggagac gttactcgat
     6481 ccgttgttgg cgacgacgag atcggctttc ccatctcggt tgaaatcagc gacagcgact
     6541 ccgtgtggac tcgagccggc gccgtaggca acggcgcttt ggaacgtacc gtttccattt
     6601 cccagaagga tcgacacatc gttggagccg ctgttggcaa ccgcgaggtc gagctttccg
     6661 tcgccattga aatctccggt gtcgacggag ttgggtgcat tgcccgcgtt gtacgttccc
     6721 gaggggagga acgtgccgtc gcccgctccc agttcgagcg aaacattgtt cgcgccgccg
     6781 tttgcggcga caacatcgag cttgccgtcg ccgttgaagt cgcccgcggc taccgacttg
     6841 ggcgtggtgc cggccgcgta actgaccgca ggagcgatcg tgttgcagtt cagcgcgcag
     6901 acgccattgt tcagaagggt gaaaaggtaa tagtcattgc tatcgatgac cgccaggtcg
     6961 ctcctgccat ctccgttgag atcgaggacg gcgatctgag cggcgccggt cgctgggtag
     7021 ttgaccgcgg cctggaacgt tccatcccct cggctcgtga gaatcgacac actgcttgca
     7081 gcattgttgg cgacggcgag gtctgggctg ccatctccgt tgacgtcgat gatcgagatg
     7141 acatgcggtc ctgacggaac ggcataattg acggcggcct ggaaggtgcc gtcgcccact
     7201 ccagccagga tcgagacgtt gttggagccg tcattggaag cggcaagatc ggctttccca
     7261 tcgccgttga aatcgccaat cgcgatcgac gaggggccgg tgcctgaggc ataaaagacg
     7321 gcggcctgca tagtaccgtc gccatttccc agcagtacag caagcttgtt ggaattgtaa
     7381 tccgctacta cgaggtcgga cttgccgtcg ccgttgagat ctgcggcgct gacccagaac
     7441 ggtccggagc cggcggcata actcacagcc gccaggaacg tgccgtcgcc ctgacccatc
     7501 aggatcgaga ccgtgtttcc gccattgttt gcgacgacga ggtcggcttt gccgtccccg
     7561 ttgaaatccg ctaccgtcac cgaaaacgga ttggagccgg cggcatagac aaccgctgtc
     7621 tggaacgtac catcgccgtt tccgagaagg atcgagacgt tgttggagat cgcatttgca
     7681 acggcgagat cgagtttgcc gtcgccgttg aaatcggcag ttgtgattga aacaggtgcg
     7741 gtgccaacgc tgtaatagct gctcgacagg aatccgccgc tgccgctacc gagcatgact
     7801 tcgatctgat tcccagacgc cgccatggcg acgtcagcct tgccgtcgcg gttgaagtcg
     7861 gccgtgacga tggcgatcgg aacggccccg atgtagttgg ttgaagcgga cccgaacgat
     7921 gggcaggctg tgatcggcgg tttcacaacg atagaaagtg tcgcagacgt cgagtggcta
     7981 tgcgcaccac
//
LOCUS       DATLVW010000623         3802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_562606, whole genome shotgun sequence.
ACCESSION   DATLVW010000623 DATLVW010000000
VERSION     DATLVW010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562606"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VNN25_18375"
     CDS             complement(<1..380)
                     /locus_tag="VNN25_18375"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004993.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HXH93554.1"
                     /translation="MEIAPDTSAINVPAHVRHARLREWVTEIATLTQPDRVYWADGTE
                     AEYEHVCEAMVLAGTLTRLNPAKRPNSYLARSDPTDVARVEERTFICSKTREDAGPTN
                     NWEDPDAMRAKLQTLFHGSMRGRT"
     gene            complement(447..1214)
                     /locus_tag="VNN25_18380"
     CDS             complement(447..1214)
                     /locus_tag="VNN25_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXH93555.1"
                     /translation="MLESAEEDRRILSQRLLIGTYQKPFLIEDAVERTLEFNLVGGSQ
                     SAMRRSDPHALIVPYTREMMAFLLWHPNPQHVVAAGLGGGSLVKYCYRHLPETRITAI
                     EINPWVISLRDEFHIPADDARLTTVCADARHYFSNPAPPADVVLIDLYDRRGAAPFTR
                     DPAFLASVRSHLTATGSVVFNVVGTDAWRRECTAAVREVFGNPVVAAQVKTDDNLVLL
                     ALKGGAAARRLTASKARFEEIKKRFGLELPAFRFALQ"
     gene            complement(1575..>3802)
                     /locus_tag="VNN25_18385"
     CDS             complement(1575..>3802)
                     /locus_tag="VNN25_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93556.1"
                     /translation="FATWLAYAYSPETLIAWTRRDEGTKRYYADQFQHVFGLPLPQAW
                     QDWITFEHEFQQRNLAEVRKFPITPEHKLAASALGSVSRMFYDAATGVLYAAVRNPGA
                     VEHVAALNTRDGSTRSLTDIKGAVHYMVTSFAYDPATGTAFYVDDTRALRDLVEVNVK
                     TGAKQLLLKHARIGEIVFNPADRSLLGVRLTNGLATLVRIPYPYKKWTVVHAFPWEQV
                     PTDLDISPDGRLLSATMSEDNGAQFLRVWELEKVLAGDLKPLSEYRFGQSVPEGFVFS
                     PDGRYLFGSSYYTGVSNIFRYEVASGTVEAVSNAESGFFRPVPLADGKLVVLTYTAAG
                     FVPAIIEPQPLKDVSAITFLGAALAEKYPVVTTWQVPAPSTVDDEKLITAKERPYVPL
                     HRVEFSNAFPVLQGYKNTVGAGYHANFEDPLHFASLGITAAYTPEEKLRSDERGHVDI
                     TGRYGFWHGELSWNRSDFYDLFGPTERSRKGYAAKLGYDWLLIYDTPRRLDLLFDVAY
                     YDKIDTLPNAQNIETNFTQLATAEIGLHYTNVKRSLGAVDDEKGVTWELVYYGSHVPG
                     ETAPQFRGGLDFGFPVPLPHSSIWLRSAAGVANGDRNSVIANFYFGAFGNNYVDDGSI
                     KRYHDYYSLPGFQINEVSALNYLREMVEWNLPPYVFEAAGTPSFYLTWLRPSLFATGL
                     WADPGSDRHHAEYQNAGGQVDLSFSVLHRYTMTLSAGYAVGREPSGRTENEWMVSLKI
                     L"
BASE COUNT          786 a         1126 c         1121 g          769 t
ORIGIN      
        1 atggtgcgcc cgcgcatgga cccatggaac aaggtttgaa gcttggcacg catcgcatcg
       61 ggatcttccc agttattggt cggaccggcg tcctctcgtg tcttggagca aatgaacgtt
      121 cgttcctcga cgcgcgccac gtcggtcgga tccgaccggg cgagatagga attggggcgc
      181 ttggcgggat tcaagcgagt gagcgtaccc gccaggacca ttgcctcgca cacgtgttcg
      241 tactcggcct ccgttccgtc ggcccaatac actcggtcgg gctgggtcag agtggcgatc
      301 tcagtgaccc actcacgtag tcgtgcgtgc cgcacgtggg cgggaacatt gatcgcgcta
      361 gtatcgggag cgatttccat tggctttctc cctcgacaaa tcgccctgta catcaagtcg
      421 cacacagcac ccgacagggt ccatgatcat tggagcgcga accgaaaagc cggcaattca
      481 aggccgaaac gcttcttgat ctcttcgaag cgcgccttac tggcggtcaa tctgcgcgcc
      541 gcggcgccgc cctttagtgc aagcaacacc aggttgtcgt cggttttaac ttgcgctgcg
      601 actaccggat tgccaaaaac ttcccgaaca gcggccgtgc attctctgcg ccaggcgtcc
      661 gtccctacga cgttgaaaac cacggagccc gttgcggtca aatgcgacct aacactggcc
      721 agaaatgctg gatcccgcgt gaatggcgcc gctccgcggc gatcgtacag atcgatcagc
      781 acgacatcgg cgggtggcgc agggttcgaa aaatagtgcc gggcgtcagc gcatacagtg
      841 gtaagtcgtg cgtcatccgc cggaatgtgg aattcatccc gcaatgaaat tacccatgga
      901 ttgatttcga tcgccgtgat gcgtgtctcc ggcagatgtc gatagcaata tttcacgagc
      961 gaccccccac ctaaacccgc tgccacaaca tgctgaggat tggggtgcca gagcagaaat
     1021 gccatcattt cgcgggtgta aggcactatt agcgcatgcg gatccgaacg gcgcatcgcg
     1081 ctttgtgagc ccccaaccag attgaactcc aaagtgcgtt caacggcatc ctcgatgaga
     1141 aagggtttct gataggttcc aatgagcagc cgctgcgaca ggattctcct gtcctcttcg
     1201 gccgattcca gcatacgcaa tactgctcgc ggcggagtga gtggcgtccg caacgttcgc
     1261 agcattggac gcttagcgac catggaagac ggcgacagta cgatgctgcg cctcgccgcc
     1321 gaggcccccg gctgttcgcc aaggcgtctt gcggtccagg actgatgttg cagtgaacaa
     1381 agggaacgac atagacacac attatatgca taattagtgt gaatatcatt atcatatcat
     1441 gtgtatgtaa ttgttctggg aacgagaatg cgcgagcact tctccgtgcg cttagtagtc
     1501 agcgttgaga atgggagtgc cggcgggcaa gcggcccgct cgttgtgttt tcctctggaa
     1561 gccgagattt tcgattacaa gattttaagc gaaaccatcc actcgttttc cgtacgcccg
     1621 gacggctcgc gacccaccgc atacccggcc gacagcgtca tggtgtagcg gtgcagaaca
     1681 gagaaactca ggtccacctg cccgccggcg ttttggtact ccgcgtggtg tcgatcgctt
     1741 ccggggtccg cccatagacc tgtggcgaac agcgatggcc gcagccacgt cagatagaag
     1801 cttggcgtgc ccgccgcttc gaacacgtag ggcggaagat tccactcgac catttcgcgc
     1861 agatagttca atgcgctgac ctcgttgatc tggaatcccg gtaacgagta gtaatcgtga
     1921 taccgcttga tggacccgtc gtcgacgtag ttgttgccaa aagcgccgaa gtagaagttc
     1981 gcgatgacgc tgttgcggtc accgttggca actcccgcag cgctgcgcag ccagatcgag
     2041 gaatgcggca gcgggaccgg aaacccaaaa tccaacccgc cgcgaaattg cggcgcggtt
     2101 tcaccgggca cgtggctgcc gtagtaaacc aattcccagg tcacaccctt ctcatcgtcg
     2161 accgcgccga gcgagcgctt cacgttggtg tagtgcaggc cgatttctgc tgtcgccagt
     2221 tgcgtgaagt tggtttcaat attctgcgcg ttgggcaggg tatcgatctt gtcgtaatac
     2281 gccacgtcaa agagcaaatc cagtcgccgc ggcgtgtcgt agattagcaa ccagtcatag
     2341 cccagtttgg cggcatagcc cttgcggctg cgctcggtcg ggccgaacag atcgtagaaa
     2401 tcagaccgat tccaggacaa ttctccgtgc cagaaaccgt agcgcccggt gatatcaaca
     2461 tggccgcgct catcgctccg cagcttttct tccggcgtgt aggcggcagt tatccccaag
     2521 ctcgcgaaat gcagcggatc ttcgaagttg gcatggtagc cggctccaac ggtgtttttg
     2581 taaccttgca gcaccggaaa ggcgttcgag aattccacac ggtgcaacgg tacatagggg
     2641 cgctcctttg cagtaatgag cttttcgtcg tcgaccgtgc tcggcgccgg cacctgccaa
     2701 gtcgtgacca cgggatactt ctccgcgagc gcggcgccaa gaaacgtgat cgcgctgacg
     2761 tccttgagcg gttgcggctc gatgatcgcc ggaacgaagc ccgcagccgt gtacgtcagc
     2821 accacgagct taccgtcggc gagcggcacg gggcggaaga agccagactc cgcgttcgaa
     2881 accgcttcga ccgtgccact ggccacttca tagcggaaga tgttggacac gccggtgtag
     2941 tagctgctgc cgaataaata gcgcccgtcc ggggaaaaca cgaatccctc gggaacggac
     3001 tgcccgaacc ggtactcgga gagcggcttc aagtcccccg ccaagacctt ctcgagctcc
     3061 cacactcgca gaaactgcgc gccgttgtcc tcgctcatgg tcgccgacaa cagccgaccg
     3121 tcgggtgaaa tatcaagatc ggtcggcact tgctcccacg gaaacgcatg cactacggtc
     3181 cacttcttat acgggtaggg aattcgcacc agcgtcgcca gaccattcgt gaggcgcaca
     3241 ccgagcaacg atcggtcggc cgggttgaac acgatctcgc cgatgcgtgc atgcttgagc
     3301 aggagctgtt tggcgccggt cttgacgttg acctcgacga ggtctcgcaa cgcgcgagtg
     3361 tcatcgacgt agaatgcagt accggtggcc gggtcgtacg caaaagacgt gaccatatag
     3421 tgcaccgcgc ccttgatgtc cgtcaggctc cgggtactcc cgtcccgtgt gttcaaggca
     3481 gcgacgtgct cgaccgcacc cggattccgt acggccgcat aaagaacgcc cgtcgctgca
     3541 tcataaaaca tccgtgatac cgaccccagc gcgctcgcgg ccagcttgtg ctcgggcgtg
     3601 atggggaatt tgcgcacctc ggcgaggttg cgctgctgga actcgtgctc aaacgtaatc
     3661 cagtcctgcc aggcctgtgg cagcgggagc ccgaagacgt gctgaaactg gtcggcatag
     3721 taacgtttgg tgccttcgtc gcggcgggtc caggcgatga gcgtttccgg tgagtaggcg
     3781 tacgcgagcc aggtggcgaa gc
//
LOCUS       DATLVW010000624         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_316470, whole genome shotgun sequence.
ACCESSION   DATLVW010000624 DATLVW010000000
VERSION     DATLVW010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316470"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..2001)
                     /locus_tag="VNN25_18390"
     CDS             complement(385..2001)
                     /locus_tag="VNN25_18390"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HXH93557.1"
                     /translation="MHRSTRRLIGLAVGLVLFLVVCALLYQLGMARLEGQHRTFWDSF
                     EWAAETLSTTGYGADSHWNHPAMVMLVVIVQFAGVFLVFLVVPIFLVPFLEERFERRV
                     PRVAPKVNNHVVVYRYGPAVETLLQRLASHSVASIVVETDEATARAVLERAQAVVFSR
                     AEEDALDICRLSEARALVANGRDEENAAITLRARQMGFRGEIFAFVEEPAHRKPMELA
                     GATAAYTPRHIVAAALAAHASERISPRLPGAEALEHLQRRELRIAAASPLAGRTLGES
                     NFGSVTGAVVAGQWMRNRLDARCDAAMRLEPGSIIEVVGDAASLERAAAMAGSPFLRN
                     TGPFLIAGFGEVGRKVHELLSDAGEEVRVVEREQRAGVDVVGNVLDPSVLERAGLREC
                     RAVVLALNSDDATLFATVIARDAAVDVPVIARVNHSRNIDNMYRAGADFALSISDISG
                     EMLSARLLGRVARSRDEHRRVVRVKIRSASDRTLRDMASRQRGCSVLAIERAGTFVPV
                     TADSRVQTDDHLYICGSAEVVNEVADKMRG"
BASE COUNT          450 a          741 c          690 g          371 t
ORIGIN      
        1 ctacggagga aaaaaaagat cctccgtagg aaagaaaagt tgctccgtag gaatccaaag
       61 atgctccgca gcaaagaaaa gatgctcgga atcggccgtt ccaggctgcg aagggtcagg
      121 cctttgcttt cctggaagtt ctaactcgtt ggcagccagt tacttgcgcg ccggagcgcc
      181 ggcgtctaag ctggctgggt caatttgaag gggagttgta acgtgacccc tcggcccttc
      241 cttccgtgca gtgtccaatg tccaattcgc ccgccgtttc ggccctctcg gctgccagtt
      301 tagccattcc aaaagcggcg ggtgacaact ccgtctcacc tccggatagg ccagcgagac
      361 tccgagaccc ccgagatcgc gacattatcc ccgcatcttg tccgcaacct cattcaccac
      421 ctcggcgctc ccgcagatgt agaggtgatc gtccgtctga actcgcgagt cagcagtcac
      481 agggacaaac gtcccggccc gctcgatcgc aagcaccgag cagccgcgct ggcgagaggc
      541 catgtcccgc agtgtgcgat ccgaagcgct gcgaatcttc acccgcacca cgcgccggtg
      601 ctcgtcgcgc gaccgggcca cacgcccgag gaggcgcgcg gaaagcatct cgccggagat
      661 gtcgctgatc gagagcgcga agtcggcgcc ggcgcggtac atgttgtcga tgttgcgtga
      721 atgattcacc cgcgcgatca ccggcacatc gacggcggca tcgcgcgcga tgaccgttgc
      781 aaacagtgtt gcgtcgtcgc tgttcagcgc caggacgacg gcgcggcatt cgcgaagacc
      841 tgcccgttcg agcacagagg gatcgaggac attgccgacc acgtcgacac ccgctcgctg
      901 ctcgcgctcc acgacgcgca cttcctcgcc ggcatcgctg agaagctcat gcactttgcg
      961 cccgacctct ccgaatccgg cgatcagaaa cggccccgtg ttgcgcagaa aaggagagcc
     1021 ggccattgcc gctgctcgct cgagcgaggc agcgtcgcca acgacctcga tgatcgatcc
     1081 cggttcgagc cgcatcgctg catcgcatcg cgcgtccagc cggttgcgca tccactgccc
     1141 ggcgacgacc gcgccggtca ccgagccgaa gttggattcg ccgagggtgc ggccggcgag
     1201 aggactggcc gcggcgatcc gcagctcgcg tcgctgcaga tgttcgagcg cctccgcgcc
     1261 gggaagccga gggctgatgc gctcactggc gtgcgcagca agagcggcgg ccacgatgtg
     1321 ccgcggtgtg tacgccgccg tggcgccggc cagttccatc ggcttgcggt gcgccggctc
     1381 ttcgacgaac gcgaatatct cgccgcgaaa acccatctgc cgtgcgcgta gcgtaatggc
     1441 ggcgttctcc tcgtcgcgcc cattggcgac gagcgcccgc gcctcgctca accggcagat
     1501 atcaagcgca tcctcctcgg cacggctgaa aacgacggcc tgagcccgct cgagcacggc
     1561 tcgcgccgtc gcctcatccg tctcgacgac gatcgacgcg acgctgtgcg acgccagccg
     1621 ctgaagcagc gtctccaccg caggcccgta gcgatagacc acgacgtgat tgttcacctt
     1681 gggagcaacc cgcggaacgc gccgctcgaa tcgctcctcc aggaacggca ccagaaaaat
     1741 cgggacgacc agaaagacga gaaaaactcc ggcaaactgg acgatcacga cgagcatgac
     1801 catggccgga tggttccagt ggctgtccgc gccgtagccg gtcgtgctca gcgtctccgc
     1861 cgcccactcg aagctgtccc agaaggtgcg atgctgcccc tccagccgcg ccatcccgag
     1921 ctgataaaga agcgcacaga cgactagaaa gaggacgaga ccaaccgcga gaccgatgag
     1981 tcgtcgcgtg gagcggtgca tgggatgatt gtagtggttg tacgtaggtc ggactctcca
     2041 gtccgacttt cacgttcagc ggaactgcca acaccagtcg gactggagag tccgacctac
     2101 gtaaaccatg tcagccctcc tcgctgataa agaagcgcac agacaacgag aaagagggac
     2161 gagaccaacc gcgagaccga tgagtcgtcg cgtggagcgg tgcatgggat gattgtagtg
     2221 gttgtacgta ggtcggactc tccagtccga ct
//
LOCUS       DATLVW010000625         9818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_480016, whole genome shotgun sequence.
ACCESSION   DATLVW010000625 DATLVW010000000
VERSION     DATLVW010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480016"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5039)
                     /locus_tag="VNN25_18395"
     CDS             complement(<1..5039)
                     /locus_tag="VNN25_18395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH93558.1"
                     /translation="MIAAILFVLPAHADVVTKADLQIQGSSLAVVDTVVTTQLGTTAF
                     VHTSFGGLQDDQAPVIEGLSAAGDLTGPGITTPIQIITAPGHAFQISGLSQEGVYYLQ
                     NVRLMNGAQLLQLAAPSAAAIQVANTLKTTVTVRQLTPDEMRARGINIDPSNYDVFEY
                     TFSFLVNGQTVTVPYPVIINRTTHQPEPGAQEDPYGLPSDTQGAPPRWVPPGNGQIII
                     LPDLLDFGDSPDPPSGPLPGKSHPQIHCAIVIPNSLAVLHQFFAVGLVVANGAPAGSN
                     VKLDSITASIDVPNQLRIAKVNPPTTFGQPVTIKDPNNPNVTFLIAQATGQADWTLEG
                     LKAGTYTVNLDVKGTFQSPGQPDLPLETKPQATVVIHDARFNITFSHPDTIRKDLQYS
                     TYTYITNISDAAQTITLSDQGLPTCSSGGYLANVCRIDTSPSSFTLTLQPGETKSVQY
                     KLQSSLTGHIFATAASIDGDNSAIASAAFQLVMGVSPTGVPLSPATLILPHYATTPYI
                     SQNLLDAELGYLGIAYSLATAPLNNQTAKFPRLITTDVFTRAVDLARAGERIFIGEDP
                     RASLSNFTLDSLGNGNPLAEWDQFLRQEVTDPAGDTARALESAMGAELSTAFSQQGGT
                     FNDMADRFAGSTTDRGPYVFALVHGPKSSTAASAFDVQVTSVATGQVLGVDATQTSGW
                     KRQIPWGGLFPLSGSVEAGTLAVIGRSNEALDLTITPSVSGAATIDLIFPAPDGVTLM
                     RASAPFTATAGQVVHIHIANGVATSNDVAPVPHAVPVAPVGIVAARQDTFLDPDGHTV
                     SVLFNRRLPATTADLSPDFDTDVVLDSSKFGVSYTGKRTVAGAALQPDRRIIHVNYDT
                     ALSSDVQYQIHTSHLVDPASNATVVPVVDPRNSALLLGTVLHGDNTIISGAQLFLTTK
                     SGRQYQLSDANGKFLFEYIPRDTDAGQNGDYNIYAVAEGRSASLDGTVRLLHTLSQIS
                     VVFLGRGSAQGTVRYEDGTRVPNATVVVGSTLFRTFRSTTTKSDGTFSVTDLPVGPLT
                     FSAQDAAGNVSYAANELHAGGEVAVQDISIYRQPFPGVGTVYGRVIRNDTNAPVANAH
                     VGVYSQGYGFSEIFTDATGTFSFPKVPAGFVTVLAANFAISPTEVAVDFDLKANESRN
                     MGDLKLVIPTANTVMVSVDGDVTAEDPLNPGLFHAVPNAQVQIKGMPVVVANAAGHYR
                     YDSVPQTVSGTDIAAYDPATRRGISKKLPDLVPYSQTTPTNNVPVAITNTSGSGKGTI
                     RVHLLSASGQPVTGYHVFWTDTAQMWSADETSLGVYEFQNLAVGTTLPIVAAPLSLSD
                     PTYGYQMTTGTATVSFADQIAAITLRLKGQGTITANIQQQTQAGVFIKLAGKLSVSFR
                     VWDNEQLDTVVHTVFANTDGTTDAIFQKVPVLPSPARVETVESAGYASADATIGYDGA
                     VVPKTLILTTLAEVRGHVFAPDGYTPVAGAAVHMFDNSQDLGIAITGFDGSFDYKNIV
                     PSSSFSVTADYTINGIYRTAITTGSTPVNGGIVDNVRLVLQQQGSVEGTVVDSNNNPV
                     PFAKIWLRELSYPARELGTAADPRFTDGTGHFRFNNIFAVSVRVTAEDPTNQESRGDW
                     GGAVTKENDVENAPITLSSTGLGSVRITVLDPNNALAPVPNAEVSLYRGALFDFGTTD
                     ASGSIQFDQLPV"
     gene            complement(5109..5915)
                     /locus_tag="VNN25_18400"
     CDS             complement(5109..5915)
                     /locus_tag="VNN25_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93559.1"
                     /translation="MRRILPITLIAFTALSAFAAGDTLLFPKPLHLTRELVDPISGKT
                     SHLEQYCFANRVISIAGARTVIADYDRSELTEIDREHGTYSVTPFSTVASARPHRKVV
                     RAATTASAASAAPAMEHRGTDRRAGRSVDLYAGDDAAHALHAEVAVDQGAALSRDAFD
                     VLTGAAYPSEGDASTELIRSASRRAVSGVSVSSTTVSESYGLPIEQTTRWNVNGRTIT
                     ATNRVTRLGDDVAPSDLVAIPPGARRVDSHIVVTARESEQLDALPGKAHP"
     gene            6268..6495
                     /locus_tag="VNN25_18405"
     CDS             6268..6495
                     /locus_tag="VNN25_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin AF2212-like protein"
                     /protein_id="HXH93560.1"
                     /translation="MQDLDAVYHDGVFEPLTAPALPEGARVHLRVESAIQGEDSLSLA
                     GQVYEGLTAEQIAEIEAIALDRSRFTTRFIS"
     gene            6604..7425
                     /locus_tag="VNN25_18410"
     CDS             6604..7425
                     /locus_tag="VNN25_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93561.1"
                     /translation="MNHGGTMLWSVRRELWENRSIYLAPLIVAALIALASLGAVRHNL
                     QPDKLAAHDELVAGVLMGTTLIVAIFYCLDALYGERRDRSVLFWKSMPVSDWTAVLSK
                     MTVPMVILPLVTFLLTIVTQSIMLLAASAYLSMKGQSAAALWNLPWLQMTSGMLYHLI
                     TVHSLYYAPIFGWLLLVSAWAKRMPFLWAFLPLAAIGFIEKIVFNTTHFATMLGSRIS
                     GGMEASPAKGAAMTMMPWPSHPLEFLTRPGLWIGLAIAATCIAAAVRLRRNREAI"
     gene            7604..8206
                     /locus_tag="VNN25_18415"
     CDS             7604..8206
                     /locus_tag="VNN25_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93562.1"
                     /translation="MQAAPSPVHWTTYLQTIMVVLSTIGAFVYVYFTYHIMKWAVGQG
                     KASVELAEISIEDTRRRLALVDNAVGNGLFQDLFRLEAINLRLERSPVDGLAVTVSEC
                     EEEIRGLLRAWEIRANEPIAANVRQLLSVPANVIGIVLAANAPLFGTELDDASVRRDR
                     LVPVVRASCALLSEILKNEFGLDFGGKTFGQIMREALPGS"
     gene            complement(8475..9488)
                     /locus_tag="VNN25_18420"
     CDS             complement(8475..9488)
                     /locus_tag="VNN25_18420"
                     /inference="COORDINATES: protein motif:HMM:NF025539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaC family protein"
                     /protein_id="HXH93563.1"
                     /translation="MAGLKYIGSLHRVRRVRTDEPLQALWQEWLKLSDDQYLKERWIG
                     STPDQRLAVTTNIRQAHEYRAASKATSLLTRPVLVYYSALNLAKAAIHLKTEAPPPDH
                     HGLMKPEAADDILDIAAKVNNGAFRSLSDLLGMTTTIGQRLTLRNFIGSIVELRCDVS
                     DYFKIRPTITPVYPDIFMDGTIRLLLKPSDLSVATVEEAIEILRKQTTLFDDFHSLRF
                     QDVMLENNKIRADELKSKGAALIEKHLDVSVFEDPRYYVNIAPEEQRIPAAPAYLVAL
                     FLLSCLVRYWPAHVQRFITTPETRVAWFVETLCATAERVLPNLLLDLIAGYRTRFVPA
                     GLL"
BASE COUNT         1754 a         2788 c         3286 g         1990 t
ORIGIN      
        1 accgggagct gatcgaactg aatcgagccg ctggcgtcgg tcgttccgaa atcgaaaaga
       61 gcgccgcgat acagcgagac ctcggcgttc ggcacaggag ccagggcgtt gttcggatcg
      121 aggacggtga tgcgcaccga gcccagacca gtggagctca acgtgatcgg cgcgttctcg
      181 acgtcgtttt ccttggtgac cgcgccgccc cagtcgccgc ggctctcctg gttcgtcgga
      241 tcctcggcag tgacgcgcac gctgacggcg aagatgttgt tgaaacggaa gtgtcccgtg
      301 ccgtcggtga agcgcggatc ggcggccgtg ccgagctcgc gcgccggata tgacagctcg
      361 cgcagccaga tcttcgcgaa gggcaccgga ttgttgttcg aatcgacgac cgttccttcg
      421 acggagccct gttgctggag gaccagcctg acgttgtcga cgatgccgcc gttgaccggg
      481 gtcgacccgg tggtgatcgc ggtgcgataa ataccgttga tggtgtagtc ggccgtgacg
      541 gagaaggagg acgagggaac gatgttcttg tagtcgaagc tgccgtcgaa tcccgtgatc
      601 gcgatcccga ggtcctgact gttgtcgaac atgtgcacgg ccgcgccggc gacgggcgtg
      661 tagccgtcgg gtgcgaagac atgtccgcgc acttcggcga gcgtcgtgag aatgagggtt
      721 ttcggcacca ctgcgccgtc gtagccaatc gtcgcgtcgg ccgaggcgta gccggccgac
      781 tcgacagtct cgacccgggc gggcgagggc agtaccggga ccttctggaa gatcgcgtcg
      841 gtggttccgt ccgtgtttgc gaagaccgtg tggactaccg tgtccaactg ttcgttgtcc
      901 cagacccgga agctgaccga cagttttccg gccagcttga tgaacacacc agcctgggtc
      961 tgctgctgga tattcgcggt gatcgtcccc tgtcccttga ggcgaagcgt gatcgcggcg
     1021 atctgatcgg cgaaggaaac ggtagcggtt cccgtcgtca tctgatagcc gtacgtcgga
     1081 tcgctcagcg agagcggcgc cgcgacgatc ggcagtgtcg tgccgacggc gaggttctgg
     1141 aactcgtaaa cacccaggct ggtctcgtcg gcggaccaca tttgcgcggt atcggtccag
     1201 aagacgtgat agccggttac cggctgcccg gatgcgctca gcaggtggac gcggatcgtt
     1261 cccttcccgg agccggaagt gttcgtgatc gccaccggta cgttgttcgt cggcgtggtc
     1321 tgactgtatg gcacgaggtc ggggagcttc ttgctgatgc cgcgtctcgt ggctgggtcg
     1381 taggccgcga tgtctgtccc cgataccgtc tgagggacgg agtcgtagcg atagtgcccg
     1441 gccgcgttgg cgaccacgac cggcatgccc ttgatctgca cctgcgcgtt cggcaccgca
     1501 tggaagagac cggggtttag cgggtcttcc gccgtgacgt cgccgtcgac gctgaccatg
     1561 accgtgttcg cggtcgggat gacgagtttg aggtcgccca tgttgcgcga ttcgttcgcc
     1621 ttcagatcga agtcgacggc aacctcggtc ggcgagatcg cgaagttcgc ggccagcaca
     1681 gtgacgaagc cggccggcac tttcgggaac gagaaggtgc ctgtggcgtc ggtgaagatc
     1741 tcgctgaatc cgtaaccctg gctgtagacg ccgacatgcg cgttggcaac cggcgcgttc
     1801 gtgtcgttgc ggatgacgcg gccgtaaacc gtgccgacgc cggggaaggg ctgccggtag
     1861 atggagatgt cctgcacggc gacctcgccg cctgcgtgga gctcgttggc agcgtaggag
     1921 acgttccccg cagcgtcctg ggcgctgaat gtgagcgggc cgaccggaag gtcggtgacc
     1981 gaaaacgtgc cgtcgctctt cgttgtggtg ctgcggaacg tccggaagag cgtgctgccg
     2041 acgacgaccg tggcgttcgg tacgcgcgtg ccgtcctcat accgcaccgt tccctgcgcc
     2101 gagccgcggc cgaggaagac cacgctgatc tgcgagagcg tgtggagaag ccggaccgtg
     2161 ccgtcgagcg aggcacttcg tccctctgcg acggcgtaga tgttgtagtc gccgttctgt
     2221 ccggcgtcgg tatcgcgcgg gatgtactcg aagaggaact taccgttcgc atcgctgagc
     2281 tgatactggc ggccgctctt ggtcgtgaga aacagttgcg cgcccgaaat gatcgtgttg
     2341 tccccgtgca gaaccgttcc gagcagcagt gcggagttgc gcggatcgac cacagggacg
     2401 acggtggcat tgctggccgg atcgaccaga tggctcgtgt ggatctggta ctgcacgtcg
     2461 ctcgacagtg cggtgtcgta gttgacgtgg atgatccgcc ggtccggctg cagcgccgcg
     2521 cccgcgacgg tgcgcttgcc ggtgtagctg acgccgaact tgctcgagtc gagcacgacg
     2581 tcggtgtcga agtccggact gagatcggcg gtcgtcgccg gcaggcggcg gttgaagagg
     2641 accgagacgg tgtggccgtc ggggtcgagg aacgtatcct gccgcgcggc gacgatgccg
     2701 accggtgcga ccgggacggc gtgcgggacc ggcgccacgt cgttgctcgt ggcgacgccg
     2761 ttggcaatgt ggatgtgaac cacctggccg gcggtggcgg tgaacggcgc ggaggcacgc
     2821 atcagcgtga cgccgtcggg cgcggggaag atcagatcga tcgtggcggc gccgctgacg
     2881 gatggagtga tggtgaggtc gagcgcttcg ttcgatcgtc cgatgaccgc gagcgttccg
     2941 gcttcgacac tgccgcttag cgggaacagc ccgccccacg gaatctgccg cttccatccg
     3001 gaggtctgcg tggcgtcgac gccgagcact tgccccgtgg ccacggatgt gacctggacg
     3061 tcgaaggcgc tcgctgccgt gctgctcttc gggccatgta ccagcgcgaa gacatacggt
     3121 ccgcggtcgg tggtcgatcc agcgaaacga tcggccatgt cattaaaggt tccgccctgc
     3181 tgcgagaagg cggtcgacag ctcggcgccc atggcgctct ccagagcgcg cgccgtgtcg
     3241 ccggccggat cggtgacttc ctggcgtaag aactggtccc actccgcgag ggggttgccg
     3301 ttgccgagcg agtcgagggt gaagttcgaa agcgaggccc gcggatcttc gccgatgaag
     3361 atccgttcgc cggcgcgcgc cagatcgacc gcgcgggtga agacgtcggt ggtgatcaga
     3421 cgcgggaact tcgcggtctg gttgttgagc ggcgcggtgg cgagactgta ggcgatgccg
     3481 aggtagccga gctccgcgtc cagcaggttc tgcgagatgt atggcgtggt cgcgtagtgg
     3541 gggaggatca gcgtcgccgg cgagagcggc acgcctgtcg ggctcacgcc catcaccagt
     3601 tggaatgccg ccgacgcgat ggcggagttg tcgccgtcga tcgacgcggc ggtcgcgaaa
     3661 atgtgcccgg tgagcgacga ctgcagtttg tactgcacgc tcttcgtctc gcctggctgc
     3721 agcgtcagcg tgaacgagct tggcgacgta tcgatgcggc agacgttggc gaggtagccg
     3781 ccgctggaac aggtgggcag accttgatcg gacagcgtaa tcgtctgcgc tgcgtcgctg
     3841 atgttcgtga tgtacgtgta ggtgctgtac tgcaggtctt tccgaatcgt gtccggatgc
     3901 gagaaggtga tgttgaagcg cgcatcgtgg atgaccaccg tcgcctgcgg cttcgtttcc
     3961 agtggtagat cgggctgccc gggcgactgg aacgtcccct tgacgtcgag attgacggtg
     4021 tatgtgcccg ccttcagccc ctcgagcgtc cagtcggcct gtcccgtcgc ctgcgcgatg
     4081 aggaacgtta cgttgggatt gttcggatct ttaatggtga ccggttgccc gaacgtggtg
     4141 gggggattga ccttggcgat gcgaagctga ttcggaacgt cgatcgatgc ggtgatggaa
     4201 tcgagcttga cgttgctgcc ggccggcgcg ccgttggcca cgaccaatcc gacggcgaag
     4261 aattggtgca gaacggccag gctgttcgga atgacgatcg cacaatggat ttgcggatgc
     4321 gacttcccag gcagaggacc cgagggcggg tcggggctgt cgccgaagtc gagaaggtcg
     4381 ggaaggatga tgatttggcc gttgccgggc ggcacccacc gcggcggagc cccctgcgtg
     4441 tccgacggga gtccgtaggg atcctcttgc gcgcccggtt caggctggtg cgtcgttcga
     4501 ttgatgatga cgggataggg aaccgttacg gtctgtccat tgaccaggaa cgagaaggtg
     4561 tattcgaaga cgtcgtaatt cgacggatcg atgttgatgc cgcgggcgcg catttcgtcg
     4621 ggcgtgagct ggcgcaccgt gacggtggtt ttcagcgtat tggcgacttg aatggccgcc
     4681 gcactcggcg cggcgagctg caggagctgc gcaccgttca tcaagcgaac gttctgcagg
     4741 tagtaaacgc cttcctgcga gagcccggag atctggaacg cgtgtccggg cgcggtgatg
     4801 atttgaatcg gcgtcgtgat gccggggccg gtcagatcgc cggcggcgga aagcccttca
     4861 atgaccggtg cctgatcgtc ctgcagacca ccgaagctgg tgtgaacgaa ggcggtcgtc
     4921 ccgagctggg tcgtcaccac ggtgtcgacg acagcgagac tcgatccctg aatttgcaga
     4981 tcggctttcg tcacgacgtc ggcgtgcgcc ggaaggacga acaagattgc cgcgatcacc
     5041 gcggcggcag actctgtaaa gcgtcttcgg aagttggaca ccggtcactc tcgtttagat
     5101 ggcggtcttc agggatgggc ttttccgggg agtgcatcga gctgttcgct ctcccgggcg
     5161 gtgacgacga tgtgggaatc gacgcggcgg gcgccgggag ggatggccac gagatcggac
     5221 ggcgcaacgt cgtcgccgag gcgcgtgacg cgattcgtgg ccgtgatggt gcggccgttc
     5281 acgttccatc gcgtggtctg ttcgatcggc aatccgtagc tttcgctgac ggtcgtgctg
     5341 cttacggaga cgcccgagac cgcccggcgc gacgcgctgc ggatcaactc cgtcgacgcg
     5401 tcgccttcgc tgggataggc ggcgccggtg aggacgtcga acgcatcgcg tgagagcgct
     5461 gcaccctgat cgacggcgac ctccgcatgc agcgcgtgtg cggcgtcatc gccggcgtaa
     5521 aggtcgacgc tgcgcccggc ccggcgatcg gtgccgcggt gctccatggc cggcgcagcg
     5581 cttgccgccg aggcagtcgt tgccgcgcgt accactttcc gatgtggccg cgcgctggca
     5641 acggtggaga agggcgtgac gctgtaggtg ccgtgctcgc ggtcgatctc cgtcagctcg
     5701 gagcggtcat aatcggcgat gacggtgcgt gctccggcaa tggagatgac gcggttcgcg
     5761 aagcagtact gctccaggtg cgaggtcttt ccagagattg gatcgaccag ctcacgtgtg
     5821 agatgcagcg gcttcgggaa gaggagcgta tccccggccg cgaaggccga gagggcggtg
     5881 aaagcgatga gcgtgatcgg aaggatgcgt cgcaatgttt gaccccttga agaaagtgtc
     5941 cgacacgcca cgcgcctgcg aagaggtaaa cgaatcctct ccgggcactc acccaaccca
     6001 ttactttccc gttgttttaa agcctggctg cgttaagagc cgcgcagtat gctcgaatca
     6061 catatatctt gtctatcgga aagtgaatca tgcatgacgt cgatcacgca gacacgtgat
     6121 ccatcgatgt ttctttcatg gtagatgact tggatcgcgg cgaagagaga cggaaagtgc
     6181 gccatatatt cgtacggctt ttcgcgatga cgtgtttgtc gctccagacc gttggcgtgc
     6241 tacagtttcc gggtgggcga atctctgatg caggatctcg acgcggtcta tcacgatggg
     6301 gtgttcgaac ccctcactgc gcccgcgctg ccagagggcg cgcgcgttca cctgcgcgtg
     6361 gaaagtgcga ttcagggcga ggactccctt tcgctggccg gtcaggtcta cgaaggtctg
     6421 accgctgagc aaatcgcgga aatcgaggca atcgcgctgg atcggtcccg attcacgacg
     6481 cgcttcattt cgtgactggc gaagttctcc tcgataccga cattcgcatt gattgtccaa
     6541 tcaagttccg agcatcgcca atcagttcgt ggcggtgatg gaggggcaag gaggagcgac
     6601 gccatgaatc acggaggaac gatgctctgg tcagtgcggc gcgagctgtg ggagaaccgt
     6661 tcgatctacc tcgccccgct gatcgtcgcg gcgctgatcg cgctcgcctc cctcggcgcc
     6721 gtcaggcaca atttgcagcc ggacaagctc gccgcgcatg acgagctcgt agccggagta
     6781 ctgatgggca cgaccttgat cgtggccatc ttctattgtc tcgacgcgct ctatggcgag
     6841 cgacgcgacc ggagtgttct cttctggaaa tcgatgccgg tctcggactg gacggcagtg
     6901 ctgtcgaaga tgaccgtgcc gatggtcatc ctgccgctgg tgacgttcct gctcaccatc
     6961 gttacgcaat cgatcatgct gctggcggcc agcgcctatc tgtcgatgaa gggtcagagc
     7021 gcagccgcgt tatggaactt gccgtggctc cagatgacat cgggcatgct ctatcacctc
     7081 atcaccgtcc actccctcta ctacgcgccg atcttcggct ggctgctgct ggtctccgcg
     7141 tgggcgaagc gaatgccctt tctgtgggcg ttcctgccgc tggccgcgat cggcttcatc
     7201 gagaagatcg tcttcaacac cacccatttc gccaccatgc tgggctcgcg catcagcggc
     7261 gggatggagg ccagccctgc aaagggagcc gcgatgacga tgatgccctg gccctcccac
     7321 cccctcgaat tcctgaccag acccggcctc tggatcggcc tggcgatcgc cgcaacatgc
     7381 atcgccgccg cagtccgcct gcgccgcaac cgcgaggcga tctagggtca ggtctaaagt
     7441 ctaaagtgcc ggcgctgtcc tgccgctgta gcgacacccg tcttgggcac tttagacttt
     7501 agacctgacc cccagagaga agccaggcat gacaggttcg ccggaggttg agacgacggt
     7561 gggagtggtc attggagttg ctgtcttggg gtagattagg ttaatgcaag ccgcgccgtc
     7621 tcccgttcac tggacgacct accttcagac gatcatggtc gttctctcga ccattggcgc
     7681 gttcgtctac gtgtacttca cctaccacat catgaagtgg gcggtgggac aaggaaaggc
     7741 gagcgttgaa ctggcagaga tatcaatcga ggatacccgt cgtcgtctcg ccttagtcga
     7801 taacgcagtt ggcaatggct tgttccagga cctatttcgg cttgaggcga tcaatctgcg
     7861 gttggaacga agtccggttg atggtctcgc ggtgaccgta tcagaatgcg aggaagaaat
     7921 cagggggctg ttgagggctt gggaaattcg tgcgaatgag ccgatcgcag cgaacgttcg
     7981 gcagctattg tcagtgcccg cgaacgtgat cggaatcgtg ctggcagcaa atgcgccgtt
     8041 attcggtacg gagcttgacg atgcaagtgt ccggcgcgac cgattagtgc cggttgtcag
     8101 ggcgtcatgc gcgctgcttt cggagatact gaaaaacgag tttgggttgg acttcggcgg
     8161 gaaaaccttt ggtcagatca tgagggaggc cctgccggga agttagtccg ggctcaaagt
     8221 cctctgaagt ggacgccgcg gagtagtcct gaagttacca gcgatcattt cagcacggta
     8281 taagtgaact caggatggtc gacgagtaga ctgtcgcaag tgcggtcaag cctaaggcct
     8341 agagtgacga cgctatcctc tcgccgtggt tacaattctt gaaccacttt agacttgaga
     8401 cctgagcccc tgagaaggca agcctacgcc tgtgttgagg caagtaggag ttcgctcctt
     8461 ccaagagatc gtcgctatag cagaccggcc ggtacgaatc gtgttcgata tccagcgatg
     8521 aggtcaagga ggagatttgg cagcacccgc tccgcggttg cacagagtgt ctcgacaaac
     8581 caagccaccc gcgtctccgg agtggtgatg aagcgctgca cgtgagcggg ccagtaccga
     8641 acgagacaac tcaagagaaa gagggcgaca agatacgccg gtgcagccgg aattcgctgt
     8701 tcttcaggtg caatgttgac gtagtagcga ggatcttcaa acacgctgac gtcgaggtgt
     8761 ttctcgatca aggcggcacc cttcgatttg agttcatctg cgcggatctt gttgttctcc
     8821 agcatcacat cctgaaaacg aagcgaatgg aaatcgtcga acaaagtggt ctgcttcctc
     8881 aggatttcga ttgcttcttc gaccgtggcc acgctcagat cgcttggctt aaggagaagc
     8941 cggatcgtgc catccatgaa gatgtcggga tacacggggg tgatggtcgg gcgaatcttg
     9001 aagtagtcgc tcacgtcgca gcgcagttct acgatgctcc cgatgaagtt gcgtagcgta
     9061 agccgctgac cgatcgtcgt cgtcatcccg agtagatccg aaagcgaacg gaacgccccg
     9121 ttgtttacct tcgcagcgat atcgaggatg tcatctgcag cttccggctt catcaagccg
     9181 tgatggtctg gaggcggcgc ttcggtcttc agatgaatcg ccgctttagc aagattcaac
     9241 gcgctgtagt agacgagtac aggacgcgtc aggagcgacg tcgccttgct cgccgcgcgg
     9301 tactcgtggg cctgacggat gttcgtagtg actgccaaac gctggtcagg cgtgctgccg
     9361 atccatcgtt ctttgaggta ttgatcatcg gacagcttca gccattcctg ccagagtgcc
     9421 tgcagcggtt cgtcagtccg aacgcgacgg acccggtgaa gtgatccaat gtatttcagt
     9481 ccagccatcg acctacagta atccgagcgg cgctaccatt caacgaagtc atggctttta
     9541 ggcttggagg ctttcgacgg ctccttatcc gcagatgacg cagatggaac gcagatggag
     9601 gacggttttc atctgagttc catctgtgtc atctgcggat cgccctcggt ttcccccgta
     9661 tcgcagcttg gcaccaccgg cgcgcacgtg cgctcctact gcgacgcaac gtggcaccac
     9721 cggaacgcat gtgcgcgcat gctgctgcaa acgttacact tcggcctctc cacgcgttgc
     9781 cgcttgacaa gtcgtctcaa ccggacttaa ccttttat
//
LOCUS       DATLVW010000626         5724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_605040, whole genome shotgun sequence.
ACCESSION   DATLVW010000626 DATLVW010000000
VERSION     DATLVW010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605040"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2105
                     /locus_tag="VNN25_18425"
     CDS             <1..2105
                     /locus_tag="VNN25_18425"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HXH93564.1"
                     /translation="RRYSSASEFASDVRHHLGDEPITAGPVSHLYRLKKFVRRHRRMV
                     IAIATAATLLAAQSIVMTVLYLRAEASRMHAELEIQRNAIDAESLQAALLGDTVRYRE
                     LTGRSQMLQRSVEPLNERALTVSAVNRLVLMKLLQDGFLADPEIEPPAGTEIDVETLV
                     GHALLSRDAQTLRTAALAAQMLKPETAGTFVDRILHILPPGAGRLDDVTQRNLEQLLE
                     TMEGQSFHPFNPAENAALEHVYRVAVERQRKTLGDRSVRMRTLRKRLAGVLEEKAQRQ
                     LQDRDSGSTATLQESLALFESVGEPPDHVARIRSDLGAAMAAQGHLQEAENLLRRALE
                     TLGSKEGMRGAYAQVAAVRLAAVLERTGDGVSATEIRTRLPRVFVEDVRELGVMRRDG
                     EIVSEPGGCSGALGNDILWAFGDRLALSNAGDAQMGELRNATAGLTLTPDEITFNRRH
                     NGSSCVDPCGAHWSLTPASIASDPRSHRFFLFYRRVLQQDSLPQQSIPSLAVCGTDLR
                     CQRTNLFAEDEPPWGSAAVVDGENLYVFACEAEGLSASCRLARVPLASVLSRRSWRFF
                     AGDGHWSTEWRDAQPILTGGMEGGILSVHRSPFFGGFVAVSSRVVDSKLSIRTAPFPE
                     GPWSDPDIEIDTLRSGPGWSWTTWGVAHPELSTDDGKVEYFTYHRRTGTMFATRIMKV
                     RFGKKTMAPVLAISEPTH"
     gene            2689..3201
                     /locus_tag="VNN25_18430"
     CDS             2689..3201
                     /locus_tag="VNN25_18430"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH93565.1"
                     /translation="MRYILLASFVSLSACVSSTSIGLPPEARGLAGHLSRVERQHLDA
                     IRYIHGGPSIIQRARESGALAENVEWWVSGPPEILPFAGTWHGLEGVSDFQKKLVATM
                     RYDKVALQRYLVSGDDVAAIFIGEGIARATGRPFHSEIVRLYTFSGGKVVRVRNYYDT
                     AAYVAAVRGQ"
     gene            3593..4333
                     /locus_tag="VNN25_18435"
     CDS             3593..4333
                     /locus_tag="VNN25_18435"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH93566.1"
                     /translation="MRRTFALVLSFFAFSATAATRDIHKALPLAADGSVSVDTHNGSV
                     TVVAWPQPSVDIAAHIEAERDDALRETDVKISDGGSSVSIVSQYPNHDFPSLAFFSFN
                     SSPTISYTIHVPAGARVRIDDHNAHTVVTGLRGDLRIHCHNGSIRVRGLGGAADIEMH
                     NGSADVEFASFTKPSRFTTHSGDFDVTLPAASRFTLNTDTRRRESVASDFPVTVTHNR
                     ASLNGTVNGGGPELRFEAHNGNLKLRRE"
     gene            4518..4850
                     /locus_tag="VNN25_18440"
     CDS             4518..4850
                     /locus_tag="VNN25_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93567.1"
                     /translation="MARPRVIGIALGALAGGTATVVAGYLAGGGHGTYVPAVALFPFA
                     MLSTRLTANVISGATITLALLQFPIYGLVTASARPTNRTRVVLTLVAVHIGVGAVSFA
                     ALRGGAFV"
     gene            5106..5300
                     /locus_tag="VNN25_18445"
     CDS             5106..5300
                     /locus_tag="VNN25_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93568.1"
                     /translation="MTALITWAPVIFLVGLWVFFMRRMGWGSKGGYREYMRISQEKIV
                     QIEQHLADIAHSLKRIADKE"
     gene            complement(5529..>5724)
                     /locus_tag="VNN25_18450"
     CDS             complement(5529..>5724)
                     /locus_tag="VNN25_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93569.1"
                     /translation="LLLGIVAAGMPVLHTVGGPRSAAHGFFFVWTLLALGATGVFSIV
                     LAAKALWLSVRDRARSPVTS"
BASE COUNT         1120 a         1750 c         1716 g         1138 t
ORIGIN      
        1 atcgccgcta ttcatccgcc tcggagtttg cgtccgacgt acggcaccac ctcggcgacg
       61 agccgattac ggcggggccg gtcagccatc tctaccggtt gaagaagttc gtgcgaaggc
      121 accgccgtat ggtcattgcg atcgccacgg ccgccactct gctcgccgct cagtcgatcg
      181 tgatgaccgt tctctacttg cgtgctgagg cttcacggat gcacgcggag ttggagatcc
      241 agaggaatgc cattgacgcg gaatcgctcc aagccgcgct tctcggcgat acggtgcgat
      301 atcgcgagct cacgggccgc tcccagatgc tgcagcggtc tgttgagcca ctcaatgaac
      361 gcgccttgac ggtgtcggca gtgaaccgtc tcgtgctgat gaagctgctg caggacgggt
      421 ttcttgcgga tcccgaaatt gagccgcccg ccggaacgga gatcgacgtc gagactctcg
      481 tcgggcacgc cctgctttct cgcgatgctc aaaccttgag gaccgcggcg ctcgccgcac
      541 aaatgctgaa acctgagaca gctggtactt tcgtcgatcg aatcctgcac attctcccac
      601 ctggtgccgg acgactcgat gatgtgactc agcggaacct ggagcaactt ctggagacga
      661 tggaagggca gtcgttccat ccgttcaacc ccgcagagaa tgcggcgctg gaacacgtct
      721 acagggtcgc agtggagcgc caacgaaaaa cgctcggcga ccgttccgtg agaatgcgca
      781 cgctccggaa gcgactggcc ggcgtcctcg aagaaaaggc gcaaaggcaa cttcaggacc
      841 gcgattcggg gtcgaccgct acgcttcagg agtcgctcgc cctgttcgaa agcgtcggtg
      901 agcctcccga tcacgtcgca cggatccggt ctgacctcgg cgccgcgatg gccgcgcaag
      961 ggcatctgca ggaagccgaa aacctgcttc gccgggcctt ggagacattg ggatcgaagg
     1021 aggggatgcg tggcgcgtac gctcaagtgg ccgctgtccg cctcgccgct gtcctggagc
     1081 gcacgggaga tggcgtgtcc gcgacagaga tccggactcg gcttcctcgc gtcttcgtcg
     1141 aggacgtccg cgaactcggc gtaatgagac gcgacggcga aatcgtgtca gagccgggcg
     1201 gctgcagcgg agcgttgggt aatgacatcc tctgggcgtt cggcgatcgt ttggctctat
     1261 cgaacgctgg cgatgcacaa atgggagaat tgcgaaatgc gacggctggg ctgactctga
     1321 ctccggatga aatcacattc aacagacgcc acaacggaag ttcttgtgtc gatccgtgcg
     1381 gggcgcattg gtcgctgact ccagcctcga tcgcctccga tccacgaagt caccgtttct
     1441 ttctcttcta tcgccgcgta ttgcaacagg attcactgcc gcagcagagc atcccatcgc
     1501 ttgccgtctg cggcacagat ctccgttgcc aaagaaccaa cctgttcgca gaggacgaac
     1561 caccctgggg atcggccgct gtcgtcgatg gcgagaatct ctatgtattc gcgtgtgagg
     1621 ccgagggtct ctccgcatcg tgccgcctcg ctcgagtgcc gctcgcatct gtcctcagcc
     1681 ggcgttcgtg gcgattcttc gcgggtgacg ggcattggag tacggagtgg cgcgacgcgc
     1741 aaccgatcct tacagggggg atggagggcg gcattctatc ggtgcatcgg agtcccttct
     1801 tcggaggatt tgtcgcggta tccagccgtg tggtcgactc gaagctctcg attcggacgg
     1861 cgccgtttcc cgagggaccc tggtccgatc ctgacatcga gatcgacacc ctgcggtccg
     1921 gtcccggctg gtcatggacc acatggggag tcgcgcatcc tgagctctcc actgacgatg
     1981 ggaaagtcga atacttcacc tatcaccgcc gtaccggaac aatgtttgcc actcgaatca
     2041 tgaaggtgag atttggcaaa aagaccatgg cgccggttct cgccatttcg gagcccacac
     2101 attagcaggc actgtcaatg tgggcgggca aatcccaagc gacggttagc cgctgtcatc
     2161 gatggttttc ggacgtcggc gaaacgatca ctaactgttc gtctcgcgac gttgttccta
     2221 tccaggcgac tgatcggtgg attgtcgccg agagcagaaa gggctcggtg atagttgtag
     2281 aaatgcatgc gcgcgagcca ccgcttacgc tctgtcgagc tctcggtgtg tgttcccgca
     2341 agtggggtca caccttcgtg cttcggtcgt tccgtatcat tttcgcgcaa gttgcagtgc
     2401 cggattgact tacggcctcg gatcccaaat tacaacaccg tcccttcgaa gaagcgactc
     2461 cgcggagaat cgacgcgttt gcaacgactt aggtacgaat tcaagcctga gcgcgcggtg
     2521 atgtctttcc ggcacaccta aaacaagtca atgaaactgc gcgagttatg tccgcgttcg
     2581 aggaggttcg tgaaaaccga aggtgtgacc ccatctcacc gcctgcgtgc ggcccaacgc
     2641 cggcgagcta gaatccgttg ggcagacgca aacctcaggg ggtgaatcgt gcgatacatc
     2701 ttgctggcat cgttcgttag cttatcggca tgcgtttcct cgacatcgat tgggctgcct
     2761 ccggaagcgc gtggcttagc cggccatctg tcgcgcgtgg aacgccaaca cctggatgcg
     2821 attcgctata tccatggagg tcccagcatc attcaacgag cacgtgaatc tggagcgcta
     2881 gccgaaaacg tcgagtggtg ggtttccggt ccccccgaga ttctgccttt cgccggcacg
     2941 tggcacgggc tcgaaggcgt ctcagatttt cagaagaaac tcgtcgcgac catgcggtac
     3001 gacaaagtcg cgctacagcg gtacctggtt agcggagatg atgtcgccgc aatcttcatc
     3061 ggcgaaggga ttgctcgcgc gacgggccgg ccctttcaca gcgagattgt tcgtctatac
     3121 acgttcagtg gtggcaaagt tgttcgcgtt cgcaattact acgacacagc ggcctatgtt
     3181 gcagcggtac gcggccagta gcggtgcgca agtggggtca caccttcgca cttcggtcgt
     3241 tccggatcat tttcgcgcaa gttgtagtgc ggattgactt acggcctcgg atccaatgac
     3301 gacaccgtcc ctgcaaagaa gcgactccgc ggagaattga cttgtttgca acgacttagg
     3361 tacgaattca acctttgagc gcgcgacgat gtttttccgg cacacctaag gcaagtcaat
     3421 gaaactgcgc gatttatgtc cgcgttcgag taggctcgcg aaaaccaaag gtgtgacccc
     3481 catctcaccc ctcccaatgt cccgctgcgc atgcgcgctg catccggctg ttcggcggcg
     3541 gttaactttt tgcgtcccca cgcgtaatca ccgataacgc gaggagatgc ccatgcgacg
     3601 tacctttgct ctcgttctct ccttcttcgc cttttccgcc actgccgcga cgcgcgacat
     3661 tcacaaagct ctgccgctgg cggcggacgg cagcgtgtcg gtcgatacgc ataacggctc
     3721 ggtgacggtc gtcgcctggc cgcagccgag cgtcgatatc gcggcgcata tcgaggcgga
     3781 gcgcgacgac gcactccgcg agaccgacgt gaagatttcg gacggcggct cgagcgtatc
     3841 gattgtgtcc caatacccga accatgactt cccgagtctc gcgttcttct cattcaacag
     3901 ctcgccgacg atcagctaca cgatccacgt cccggccggc gcgcgcgtca gaatcgacga
     3961 tcacaacgcg catacggtgg tgaccggctt gcgcggcgac ctccgcatac actgccacaa
     4021 cggctcgatc cgcgtccgcg gcctcggcgg cgcggccgac atcgagatgc acaacggcag
     4081 cgcggacgtc gagttcgcgt cgttcacgaa accgagccgc ttcacgacgc acagcggcga
     4141 cttcgacgtc acgttaccgg ccgcatcgcg cttcacgctc aataccgaca cacgacggcg
     4201 ggaatcggtc gcatctgatt ttccggtgac cgtcacgcac aaccgcgcgt cgctcaacgg
     4261 aaccgtgaac ggcggcggcc ccgagctccg cttcgaagcg cataacggga atttgaagct
     4321 gcgcagagag tagggaggca cgacgcaata gggtcacgcc ttcgctccgt gggtcctgcc
     4381 tccggaggga agggtcacga tgtctccatc gcggagaaca ctccgattgg aacatagcgt
     4441 ttgatagtcg tcagccgaac gaccgaaggt gtgacccctc tggcaccttt gaacccaggc
     4501 ctagctactc gagtcatatg gcacgtccac gcgtcatcgg aattgcgctg ggagccttgg
     4561 ccggcggaac ggctacggtc gtggcaggct acttagccgg cggcggccac ggtacctacg
     4621 tgcccgcggt ggccctattt cccttcgcca tgctctctac gcggctgact gccaacgtca
     4681 tctcgggtgc cacgatcacc ttggcccttc tgcagtttcc gatctacggg ctcgtaacag
     4741 cgtccgcacg accaacgaac cgcacacgcg ttgtcctcac tctcgttgcc gttcacattg
     4801 gcgttggcgc agtctctttc gccgctcttc gtggcggagc attcgtctaa atcaaaaggg
     4861 cttcgcgcgc ggcgccgtcc aactcatgcc tgcagccgac gtccgcggcc ctgtcagcgt
     4921 aggtgctacg atcacgagtg cgggctacgc ggctgaggcg tagattgttg ggcggcacca
     4981 gccatccgta ttcgaatgga ggcaacttga gtcatcgtcc tcattgggtc gcaacgctgt
     5041 gtctcttctc cctggcgagc atgccagcgt tcgccgcaaa caatcagccg ccggactcac
     5101 ccctcatgac cgctctcatc acgtgggcgc cagtcatttt cctggtcggg ttgtgggtct
     5161 tcttcatgcg cagaatgggt tggggatcga agggcggtta ccgcgagtac atgcggatat
     5221 cacaagagaa gatcgtccag atagagcagc acctcgcgga catcgctcac tcgctgaaga
     5281 gaatcgcgga caaagagtag cgaaggatcc cgttcgctcg cggtgaagcg tatggggtca
     5341 ggtctgaagt gtgaagtgac ggaagatcga tcgcggcaag aaccgaaaag cgggcgccgt
     5401 cacttcacac ttcagacctg accccttcca ctcgacgatg tcgccaacgc ggagaagagc
     5461 ccgtttgaaa gatagcgttt gacggtcgac agcagaacga ccgaggtgtg acccctctgg
     5521 cacccacact atgaagtcac cgggctccgg gcgcggtcgc gaacagacaa ccagagtgcc
     5581 ttggccgcga gcacgatgga gaacacgccg gtggcgccga gcgcgagcag cgtccagacg
     5641 aagaaaaaac cgtgagccgc gctgcgcggt cccccgacgg tgtgcaacac aggcatgccg
     5701 gcggcgacga tcccgagcag cagc
//
LOCUS       DATLVW010000627         4015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_437499, whole genome shotgun sequence.
ACCESSION   DATLVW010000627 DATLVW010000000
VERSION     DATLVW010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437499"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4015)
                     /locus_tag="VNN25_18455"
     CDS             complement(<1..>4015)
                     /locus_tag="VNN25_18455"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HXH93570.1"
                     /translation="EQNVDAESILSAVFSKPIDTGTSAAPDDVDTYLHQHNFFTVWMG
                     PALPPGTAAGTPLAWTDITQQAKFSSDSGGRRVKIDVGASFQRGATYRVQLNKAITSV
                     PASGSSTAPLEIGQVRDSKTGTVSGGLTDPLVLWFKVRDVATLGAFKLRQDSSTSKGN
                     IRDYALNGNVLFVAALDGGLLAYDAADPASLNESTSTPPLPLGYVAGSQNFWSVATDQ
                     HGRVYTTGAGSTFNSINSFRIDDFVGHDGAVLSSPRVVQPRSGGIVSWAVGYNAGFDV
                     ELVLSNRPEGIPRKLQLAEQDTTIDLGTLQHFIDSPPAGVTITNQSTTNGIVQLKVHV
                     QKEPGFPYVSQRITIENTTRDMRWSVDAYDSGFPTPDIDYIVGDPGDTLRLIYNERTY
                     GIVTIFGYGIGVFDLNAIESNDDRQHQPSGYQAISEAVKITAADVLSTPIVSTIGETS
                     CQPVNDPKAIPDLSLSPDITLLSGMNATTNNPTLRAFGLDVHRGALDDVITPPATTSG
                     TAAANQCDDRAPIGLLLGPPTISGAPNPNVSPRIKALVVPFQTAAGGRIPAARFNGIA
                     TYHWRLEAQDNAALPAAAGTPPPPTWGQRHSTAGQAVDRDYLLIPGYELGLIVLDVTY
                     PATAHIDNSSVADIIWIPGGAVGVRVIPRSHYATVVDANGHVLIVDLSNIDQRWDSHG
                     TELPADALFKNVAAALTGTSAYGTGALDPRVVWISPNPIVSSTLPPVIDIDTGIMFAG
                     SVGDQMTKVIAAFDPQIEIRADTGRGGTSQVGGVVPLGVDPPAGALVAAAAGTPPNNA
                     SLGAFRLQITLPGGVAEKLANQILQLAVESERVPGAVVEQTPEVNFSKPFPRSSLRQV
                     RPDGSSDNRAVPATFQLTRFAPANIAAALRRQKGFNTFVSPWIVAIADPRASISYNWN
                     GGSPKDFGCYTCTRPPLLQGKTEADGVFEIWSGGRDFKVRPELVATAPGTTLKTIFDS
                     TGYGYLAQGDRLVKRFPTIMGDTTRGLDVVAAGQNPPAAGGAIQETILLHSGELTTSN
                     VDLAPGGRAGWDVVVDRHYRSRTMLPSPFGLGWTSSLFRHLRALPNGSVEYRDETGEV
                     FLFNAPASGADHYGQPKGLLMRLVRTDNGWQMIDMKRRISSFDNYGRLVTESDEFGDP
                     GISPTNGNSIRYIYDLTGRLVQVIDPVNRATKIDYWDDSMLTGWKHGRLKTITDWRNR
                     KVDYDYDDDGFGGGRLTKVQLADVPDDFENRRPTIIYTPATIPGTSYNDGIELDPELK
                     AISDPSFNDGTNAGTSRVTFSYNTANGPDPDRAKVTGQTWPTGDSVSISAPYGASPTV
                     TDARGHARTYTFTSAAKP"
BASE COUNT          637 a         1163 c         1463 g          752 t
ORIGIN      
        1 ccggctttgc ggcgctggtg aacgtgtagg tgcgcgcgtg gccgcgtgca tcggtgacgg
       61 tcggtgacgc gccgtacggc gcggagatgg agacgctgtc tccggtgggc cacgtctggc
      121 cggtgacctt ggcgcgatcg gggtcggggc cgttggccgt gttgtagctg aacgtgacgc
      181 ggctcgttcc agcattcgtg ccgtcattga acgacggatc ggagatcgcc ttcagctccg
      241 gatcgagctc gatgccgtcg ttgtacgaag taccagggat cgttgccggc gtgtagatga
      301 ttgtcgggcg gcggttttca aagtcgtccg gcacgtcggc gagctgcacc ttcgtcaggc
      361 ggccgccgcc gaacccatcg tcgtcgtagt cgtaatcgac tttgcggtta cgccaatcgg
      421 tgatcgtctt caagcggccg tgtttccagc cggtgagcat cgaatcatcc caatagtcga
      481 tcttcgtggc gcggttgacc ggatcgatga cctgcacgag ccgtccggtg aggtcgtaga
      541 tgtagcgaat gctgttgccg ttggtcggcg aaatgcctgg gtcgccgaac tcgtccgact
      601 cggtgacgag gcggccgtag ttgtcgaagc tggagatgcg acgcttcatg tcgatcatct
      661 gccagccgtt gtcggtgcgg acgaggcgca tgagcagtcc cttcggctgt ccgtagtggt
      721 cggcgccgga cgccggcgcg ttgaagagga agacctcgcc ggtctcgtcg cggtactcga
      781 cggagccgtt tggcagcgcg cggagatggc ggaagagcga agaggtccat ccgagtccga
      841 acggactcgg aagcatggtg cgggaacggt aatgacggtc gacgacgaca tcccagccgg
      901 cgcggccgcc gggggcgagg tcgacgttcg aggtcgtcaa ctcgccggag tggagcagga
      961 tcgtctcctg gatcgcgccg cccgcggcgg gaggattctg cccggcggcg acgacgtcga
     1021 gtccgcgcgt ggtgtcgccc atgatggtgg ggaagcgctt gaccaggcga tcgccctgcg
     1081 cgagatagcc gtatccggtg ctgtcaaaga tcgtcttgag cgtggtacct ggggcggtgg
     1141 cgacgagctc cggacggacc ttgaagtcgc ggccgcccga ccagatctcg aacacgccgt
     1201 cggcttcggt ctttccttgc agcaatggtg gacgcgtgca cgtgtagcaa ccgaagtcct
     1261 tcggcgatcc gccgttccag ttgtacgaga tgctcgcgcg cggatcggcg atggcgacga
     1321 tccacggcga gacgaaggtg ttgaatccct tctgccgtcg cagcgcggcg gcgatgttgg
     1381 ccggtgcgaa gcgggtcagt tggaaggtgg ccggaacggc gcggttgtcg ctggagccgt
     1441 ccggacgcac ctgccgcaag ctcgagcgcg ggaagggctt gctgaaattg acctcgggcg
     1501 tctgctcgac gaccgcgccc ggtacgcgct cgctctcgac ggcgagctgc aggatctggt
     1561 tggcaagctt ctcggcgacg ccaccgggca gcgtgatctg cagccggaac gcgccgaggg
     1621 aggcgttgtt cggcggcgtt cctgccgctg cggcaacgag agcgcccgct ggaggatcga
     1681 cgccgagcgg tacgacgccg ccgacctggc tcgtaccgcc acggccggtg tcggcacgaa
     1741 tctcgatctg cggatcgaat gcggcgatga ctttggtcat ctggtcgccg acagagccgg
     1801 cgaacatgat gccggtatcg atgtcgatga cgggcggcag agtgctgctg acgatcggat
     1861 tcggcgagat ccagacgacg cgcgggtcga gcgcgccggt gccatacgcc gacgttcccg
     1921 tcagcgcggc cgcgacgttc ttgaagagag catccgccgg aagctcggta ccgtgcgaat
     1981 cccatcgctg gtcgatgttc gaaagatcga cgatgagcac atggccgttg gcgtcgacga
     2041 cggtggcgta atggctgcgc ggaatgacgc gtacaccgac ggcgcctccg gggatccata
     2101 tgatgtcggc gacactgctg ttgtcgatgt gcgcggtggc cgggtaggtg acatcgagaa
     2161 cgatcaggcc gagctcgtat ccggggatga ggaggtaatc gcggtcgacc gcttgtccgg
     2221 cggtggagtg acgctggccc caggtcggag gaggtggcgt acccgctgcg gctggcagcg
     2281 cggcgttgtc ctgcgcctcg aggcgccagt gatacgtcgc gattccgttg aagcgagcgg
     2341 ccggtatgcg gcctcccgcc gccgtctgga atgggacaac cagtgctttg atgcgtggcg
     2401 acacgttcgg attcggcgcc cccgatattg tcggcggacc gagaagcaat ccgatgggag
     2461 cgcggtcgtc acactgattg gcagcggccg tgccgctcgt cgtcgccgga ggcgtgatca
     2521 cgtcatcgag cgcgccgcga tgcacatcga gaccgaacgc tcgaagcgtc gggttgttgg
     2581 tcgtggcatt catccccgac agaagcgtga tgtcgggcga gagtgacagg tcgggaatgg
     2641 cctttggatc gttgaccggc tggcatgacg tctcgccgat ggtcgagacg atcggtgtcg
     2701 acaggacgtc ggccgcggtg atcttgacgg cctcgctgat ggcctggtaa ccggacggct
     2761 ggtgttgccg gtcgtcgttc gactcaatgg cgttgagatc gaacacgccg atgccatagc
     2821 cgaagatcgt gacgatgccg taggtgcgct cgttgtagat caggcgcagg gtgtcgcccg
     2881 ggtcgccgac gatgtaatcg atgtccggtg tcgggaagcc ggagtcgtag gcgtcgaccg
     2941 accaccgcat gtcgcgggtg gtgttctcga tcgtgatgcg ctgcgatacg taggggaacc
     3001 cgggttcttt ctggacgtga accttaagct gaacgatgcc gttcgtcgtg ctctggttcg
     3061 tgatcgtcac gccggcaggc ggcgagtcga tgaagtgctg caacgtgccg aggtcgatgg
     3121 tggtgtcctg ctcggcgagc tgcagctttc ggggaatgcc ctcgggccgg ttgctcagca
     3181 ccagttcgac atcgaatccg gcgttgtagc ccacagccca cgacacgatg cctccggagc
     3241 gcggctgtac gacgcgcggc gacgagagca cggcgccgtc gtggccgacg aagtcgtcga
     3301 tgcggaagct gttgatggag ttgaaagtgc tgccggcgcc ggtcgtgtag accctgccgt
     3361 gctggtcggt ggcgaccgac cagaaattct gcgaccctgc gacgtaaccg agcggcagag
     3421 gtggcgtcga cgtgctttcg ttgagcgacg cgggatcagc agcgtcgtag gcgaggagac
     3481 cgccgtcgag cgcggcgacg aagaggacgt tgccgttgag ggcgtagtcg cggatgtttc
     3541 ccttcgacgt cgagctgtcc tggcgcagct tgaaggcgcc gagcgttgcc acgtcgcgaa
     3601 ccttgaacca gagaaccagc gggtcggtga gtccgccgct gacagtgcca gttttgctgt
     3661 cgcgcacctg gccgatctcg aggggcgctg tgctcgagcc gctggcaggc acgctcgtga
     3721 tcgccttgtt caattggacg cggtacgttg cgccgcgctg gaatgacgcg ccgacgtcga
     3781 tcttcacccg ccggccgccg gagtcgctgc tgaacttcgc ctgctgggtg atgtccgtcc
     3841 aggccagcgg cgttccggcc gcggtgccag gcggcagcgc cggccccatc cagacggtga
     3901 aaaagttgtg ctggtgcagg taggtatcaa catcgtccgg cgcggcgctg gtgccggtgt
     3961 cgatcggctt gctgaagacg gcgctgagga tcgactcggc gtcgacgttc tgctc
//
LOCUS       DATLVW010000628         3275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_109564, whole genome shotgun sequence.
ACCESSION   DATLVW010000628 DATLVW010000000
VERSION     DATLVW010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109564"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1614)
                     /locus_tag="VNN25_18460"
     CDS             complement(130..1614)
                     /locus_tag="VNN25_18460"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH93571.1"
                     /translation="MFRRHISILVPCAWLLTMGASALHADAAASTAIAPPQRLNLAPC
                     KLPGIERQMRCGRFDVFEDRKRQSGRTISLRVVVLPASSPDRASDPIFAFEGGPGMSA
                     VESAADELAANADLNRTRDLVLVDLRGTGDSNGLQCKSLQGHKGVLGFLESFLPVDGV
                     RACRLALEKRADLKFYTTTLAVDDVDDIRAALGYDRINLEGDSYGTFAALNYMRRHPE
                     HVRTAVLQGIVPPDTRMPLYFAHDAQTALDQGLAACARDFACHTAFPDPAGELDRLLA
                     KLERQPVEATFKEPSDGRQIKLVLGRSAVAQTIRYMLYTPVTAAQIPLQIHLATQGNF
                     TPIAERAYFFGNFVASLSDGFYLSVTCAEDVPLYTLAEAEKAAKGTFLGDFRARAQKA
                     ACAAWPQGETPAGFTQPVRSETPTLLLSGERDPVTPAAWAEQAARTLPHSRHIVVQGG
                     GHGFGGEKGIECLDRTKDFIQRGSEKNLDTSCTAKIEPVPFVLQ"
     gene            complement(1789..2049)
                     /locus_tag="VNN25_18465"
     CDS             complement(1789..2049)
                     /locus_tag="VNN25_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93572.1"
                     /translation="MTRHAVRTTAGKMNRSDDLLADEKEVAAAVLRANPDFGNPHVVR
                     CDWCHETRTLFRDENEAPFKLTTHGWLCETCNVRAASQEVRF"
     gene            complement(2076..>3275)
                     /locus_tag="VNN25_18470"
     CDS             complement(2076..>3275)
                     /locus_tag="VNN25_18470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXH93573.1"
                     /translation="RLSTAAFADWCAVDLLNDDGSIRHLTVSHANPVLRDLALQFQQV
                     AFSEPENAPGAPQALRLRKSVLLTDIANSQESGIRPAAKVTQLIGALGLRSLISVPLI
                     VRDKVLGTTTFSSSRRQYEPLDLQLAEELSQRAAIAIDTAMLFREAESANRYKDAFIG
                     TVAHELRTPLTSIIGWVQLGLRNHDLSNEALLRVDESANLLRVFTEDLLDVTRIREQK
                     LSVAMETIDFATVVRSAIEMTAISAAAKEIKVSSHLLLDPAPLTGDRVRLLQVVWNLL
                     SNAIKFTDPGGSIDLRLEQYGNAARLSVMDTGTGISADFAPHVFELYRQAAGSPGHLP
                     GLGIGLSIVAQILKLHGGTVRVESAGIGHGSTFIVTLPFPVPSEPATPIGRRSKSAKH
                     VRLDRRS"
BASE COUNT          578 a          946 c         1114 g          637 t
ORIGIN      
        1 cgcccatcga gacctccaat ctttcgccgt gccgttgcac gaccagctca ttgccgcttg
       61 gggctggcgt aggtgccacc gaaccgatcg aggtcagctg acgagagctg tatctctgct
      121 gcgcgctcgt cactgtagga cgaatgggac cggctcgatc ttggcggtgc agctggtgtc
      181 gaggttcttt tcgctgcctc gctggatgaa gtccttcgtg cggtcgaggc actcgatccc
      241 cttctcgcca ccgaatccgt ggccgcctcc ctggacgacg atgtgtctgg aatgtgggag
      301 ggtgcgtgca gcctgttcgg cccaggctgc cggagtcacc gggtcacgct cgcctgagag
      361 caggagggtc ggcgtttcgg agcgcaccgg ctgcgtgaag ccggccgggg tttcgccttg
      421 cggccacgcc gcacaggccg ccttctgcgc gcgcgcgcga aagtcgccca gaaaagtacc
      481 cttggctgcc ttctcggcct cggcgagcgt gtagagggga acatcctcgg cgcaggttac
      541 cgagagatag aacccgtcag acagagacgc aacgaagttg ccgaagaaat acgctcgttc
      601 agcgattggc gtgaagttgc cttgtgtcgc cagatggatt tggagaggga tttgtgccgc
      661 ggtaaccggc gtgtagagca tgtaacggat tgtctgcgca acggcgctgc ggccgagcac
      721 gagtttgatc tgacgtccgt cactcggttc tttgaaggtg gcctcgacgg gttgcctttc
      781 aagcttggcg aggaggcgat cgagctcgcc ggctggatcg gggaatgccg tgtgacaggc
      841 gaagtctctg gcgcacgcgg cgagcccctg gtcgagtgcc gtctgggcat cgtgggcgaa
      901 gtaaagcggc atgcgagtgt cgggggggac gatgccctgg agcacggccg ttcttacgtg
      961 ctcaggatgc cggcgcatgt aattcagcgc cgcgaacgtg ccgtaagagt ccccctcgag
     1021 gttgatgcgg tcataaccta gcgctgcgcg gatatcgtct acgtcatcga cggccaaggt
     1081 cgtcgtgtag aacttcaaat ccgctcgctt ctcgagcgcc aagcggcagg cgcgaactcc
     1141 atccactggc aggaagctct ccaggaaacc aagcaccccc ttgtgtccct ggagcgactt
     1201 gcactgcaat ccgttcgagt cgcccgtgcc gcgcaggtcg acgagtacaa ggtctcgtgt
     1261 gcgattgaga tcggcgttag ccgcgagctc gtccgctgcc gactcgacgg ccgacatgcc
     1321 ggggccgcct tcgaacgcga agatgggatc ggatgcacgg tcgggactgc ttgcaggcag
     1381 cacaacgact cgcagagaaa tcgttctacc cgactgccgt ttgcgatctt caaagacatc
     1441 gaaacgccca cagcgcatct ggcgctcgat tccaggaagt ttgcaaggcg cgaggttgag
     1501 acgctgcggc ggagcgatgg ccgttgatgc agctgcatca gcgtgcagcg ccgaggcgcc
     1561 catggtcaag agccaggcgc acggaaccag gattgagatg tgacgcctga acatggcgct
     1621 gtacccacct ttcgatgtcg ggtcgtatcg gaatgagaac gatggcgcga ttgatcggtt
     1681 aatgagcgta gcttggataa gaatgtgccg cctatccttc tgagtggagg ccctctccgt
     1741 acactatccg aagtggcgcg cagtcgcatc gtcaggctgg aacactattt aaaacctcac
     1801 ttcctggctt gccgccctca cattgcacgt ctcgcagagc cagccgtggg tggttagctt
     1861 aaagggggct tcgttttcgt cgcggaagag ggtgcgggtt tcgtggcacc agtcgcagcg
     1921 gactacgtgg gggttgccaa agtcggggtt ggcgcggagg acggcggcgg cgacttcttt
     1981 ttcgtcggcg agcagatcgt cgctgcgatt catctttccg gcggtggtgc gaacggcgtg
     2041 tcgggtcaag gaagctcctt tactgatcgc ggtgactacg aacgcctatc caggcgtacg
     2101 tgtttggcgc tcttgctgcg gcgcccgatg ggtgttgcgg gctctgatgg aacggggaac
     2161 ggcagcgtca cgatgaacgt cgatccgtgg ccgatgccgg cgctctcgac gcgcaccgtc
     2221 ccgccgtgga gtttcaggat ctgcgcgacg atggagagac cgatgccgag gccggggagg
     2281 tggccgggag agcccgcggc ctggcggtac agctcgaaca cgtgcggtgc gaagtcggcg
     2341 ctgatgccgg tgccggtatc catgaccgac aaccgcgcgg cattgccgta ctgctccagc
     2401 cgaaggtcga tcgaaccgcc cgggtcggtg aacttgatgg cgttgctgag gaggttccag
     2461 acgacctgga ggagccggac gcgatcgccg gtcaacggag cgggatcgag caggaggtgc
     2521 gaagaaacct tgatctcttt cgcggcggcg ctgatcgccg tcatctcgat cgccgagcga
     2581 acgaccgtcg caaagtcgat ggtctccatc gccacgctca gcttctgttc gcggatgcgc
     2641 gtgacgtcca gcaggtcctc ggtgaagaca cggagcaggt tcgcgctctc gtcgacgcga
     2701 aggagtgctt cattgctcaa gtcgtggttt cgcagcccga gctgtaccca gccgatgatc
     2761 gatgtcagcg gagttcgcag ttcgtgcgcg acggtgccga tgaacgcgtc cttgtatcga
     2821 ttggcgcttt cggcttcccg gaacagcatg gccgtgtcga tggcgatcgc agcgcgttgc
     2881 gacaactcct cggcgagctg caggtcgagc ggttcgtact ggcgtcgcga cgacgaaaac
     2941 gtggtcgttc cgagaacctt gtcacggacg atgaggggca cgctgatcag cgaccgcaat
     3001 ccgagggcgc cgatgagttg cgtgaccttc gccgcgggac ggatgccgct ctcttgcgag
     3061 ttggcgatat ccgtcagcag taccgatttg cgcagacgaa gtgcttgcgg cgcgccgggc
     3121 gcattctccg gctcgctgaa cgctacctgc tggaactgca aggccagatc gcgcagcacc
     3181 ggattggcat gcgacacggt gaggtggcgg atcgagccgt catcgttgag caggtcgacg
     3241 gcgcaccagt cggcgaatgc tgcggtggac aggcg
//
LOCUS       DATLVW010000629         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_25259, whole genome shotgun sequence.
ACCESSION   DATLVW010000629 DATLVW010000000
VERSION     DATLVW010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25259"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VNN25_18475"
     CDS             <1..455
                     /locus_tag="VNN25_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93574.1"
                     /translation="FDPVSAAMASADITLAALLDARRAARLVQFHAEDAANPDFNEII
                     NDVIATVTRRESGYRGAITRASARLTATRLMNLAANADADPQVRAEASEGLRRLSGKL
                     ADAGIRDDAEVAHRHAMRDDIERFLARPDQPRALPKLPEVPPGPPIGD"
     gene            645..1097
                     /locus_tag="VNN25_18480"
     CDS             645..1097
                     /locus_tag="VNN25_18480"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXH93575.1"
                     /translation="MVEPVLMGHAPAKVDEKGRLKIPSNFRKVIEEQHGSECFITSMD
                     GERALIYPMAVWYAYQGRLSKVPSSSVAKNKLMEKVNFFGHAATIDGQGRVLIPTILR
                     NVAGITEDVVVLGNTDHLIVENGEKIQQRMSETPMSAEDWKELELHGV"
     gene            1104..2036
                     /gene="rsmH"
                     /locus_tag="VNN25_18485"
     CDS             1104..2036
                     /gene="rsmH"
                     /locus_tag="VNN25_18485"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXH93576.1"
                     /translation="MHQSVLLSEIVEFLRPSRDDGTLVDATIGLGGHAEALLTRYPSI
                     RLLGIDRDPAALAASAARLEKFGDRVTLAQGRHETLIDILKKQKIEAVSGLLADLGVS
                     SMQLDDASRGFSFRYDAPLDMRMGPESTTAAELVNTLGERELATILREYGEEPMARGI
                     ARAIVNARAEGPIETTARLAEIVRSVKKPRFREIDPSTLTFQALRIAANEELVGLDKF
                     VDDAVSVLEKGARIAIIAFHSLEDRIVKRALRRLEGECTCPPNLPVCGCGAKEVVKIL
                     TSRPVTASEEEIDRNPRSRSAKLRVAEKLLAVSS"
     gene            2285..2671
                     /locus_tag="VNN25_18490"
     CDS             2285..2671
                     /locus_tag="VNN25_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93577.1"
                     /translation="MYAQKKSIENSHIVRERDRKRFRELLAVLGLGVPIGLFLLLFTW
                     QNLEVIRLGHEATRLQKKKQEIENANRALQLELDRRTSLGAVEQRATELGFQHTDPRV
                     VVMVQTPPADSAPTPHPPQPTPAGSR"
     gene            2671..>3111
                     /locus_tag="VNN25_18495"
     CDS             2671..>3111
                     /locus_tag="VNN25_18495"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HXH93578.1"
                     /translation="MPISQKRLLQLFAFLAAWALVVIVRLAQVQLVRHGDYVVKAQRQ
                     QERTLALQPVRGSIFDSRQRVLAESVAAESIYADPQAIGDRAQTAKALASVAALQLSA
                     AELNTKLASSGGFVWIARQLPLEVSAAVKALRLPGIYYLEEHRRS"
BASE COUNT          601 a          947 c         1012 g          551 t
ORIGIN      
        1 cgttcgatcc ggtgagcgcg gcgatggcgt cggccgatat cacgcttgcc gccctgctcg
       61 atgcccgccg cgccgcgcgg ctcgtgcaat ttcacgccga ggatgctgcg aatcccgatt
      121 tcaacgagat catcaatgat gtcatcgcaa cggttacgag acgtgagagc ggctaccgcg
      181 gggcgatcac ccgcgcctcg gcacgcctga cagcgacgcg gctgatgaac ctcgccgcga
      241 acgccgacgc cgatccgcag gtgcgtgccg aggcgagcga aggcctgcgc cggctgtcgg
      301 ggaagctcgc cgacgccgga attcgcgatg acgccgaagt cgcgcatcgt cacgccatgc
      361 gcgacgacat cgagcgcttt ctcgcacgcc cggaccagcc gcgcgcgctg cccaagctcc
      421 ccgaggtgcc gcccgggccg ccgatcgggg actgaactaa cgcggaagac cctttaacca
      481 caaaggcaca aaggacacag aggagtcatc tcctttgtgt cctttgtgtc tctgtggtta
      541 aagccttttc agaccccctt ttttctacgg tgagtggatt tttgtggaca gtagtggtcg
      601 ggagtggtac aaagaggctt cgaaacacga aagtcagagc ggaaatggtc gagccagtct
      661 tgatggggca tgccccggcg aaggtcgacg agaagggtcg cctcaagatc ccctcgaact
      721 tccgcaaggt cattgaagag cagcacggct ccgagtgctt catcacttcg atggacggag
      781 agcgggctct catctatccc atggcggtct ggtacgcgta ccagggacgc ctttccaagg
      841 tgccgtcgtc gtcggtggcg aagaacaagt tgatggaaaa ggtgaacttc ttcggtcacg
      901 cggcaacgat cgatgggcag gggcgggtgc tgatcccgac catccttcgc aacgtcgccg
      961 gcatcaccga agacgtcgtc gtcctcggga acacggacca cctcatcgtc gagaacggcg
     1021 aaaagatcca gcagcggatg tccgagacgc cgatgagtgc cgaggattgg aaagagttgg
     1081 agttgcacgg tgtgtgagat gtcatgcacc agtcggttct tttaagcgaa atcgtcgagt
     1141 ttctccgtcc ctcgcgcgat gacggaacgc tcgtcgatgc cacgatcggg ctcggtggac
     1201 acgcggaagc actgctgacg cgctacccat cgatccggct cctgggcatc gaccgcgatc
     1261 cggcggcgct ggccgcgtcg gcagcgaggc tggagaaatt cggagaccgc gtgacgctcg
     1321 cacagggccg ccacgagacg ctcattgaca tcctgaagaa gcagaagatc gaggcggtca
     1381 gcggattgct cgcagacctc ggcgtctcgt ctatgcaact cgacgacgca tcgcgcggat
     1441 tctcgttccg ctacgacgcg ccgctcgata tgcggatggg gccggagtcg acgacggccg
     1501 cggaactggt caacacgctc ggggagcgcg agctcgccac gattctgcgc gagtacggcg
     1561 aagagccgat ggcgcgcggt atcgcacgag cgatcgtgaa tgcgcgtgcc gaggggccga
     1621 tcgaaacgac ggcacggctg gcggagatcg tgaggagtgt gaagaagccg cggtttcgcg
     1681 agatcgatcc atcgacgctc acgttccagg cgcttcgcat cgccgcgaac gaggagctgg
     1741 tgggtctcga caagttcgtc gacgacgccg tgagcgtgct cgaaaaaggg gcgcgtatcg
     1801 caatcatcgc gttccattca ctcgaggatc gcatcgtcaa acgggcattg cggcggttgg
     1861 aaggggagtg cacctgcccg ccgaacctgc cggtttgcgg gtgcggcgcg aaagaggtcg
     1921 tgaagattct gacaagccgt ccggtcactg cttcggagga agaaattgat cgcaatccgc
     1981 ggtcgcgcag cgcgaagttg cgggtggcgg aaaagctctt agctgtcagc tcttaggaaa
     2041 tcgaagttct gagagttgac aggtaagtgc tcgcagctcg gagtcgaagg agctggagcg
     2101 cgtagtaacc ggagcccgga aacaagggtg aaggttgggg aagggtgggc gtgtgtgcgc
     2161 cgcgggagct cgggaggggt ggctggggga gcgtgggtgg gtgtggatgg tttccgggtc
     2221 gtgcttgtag gcagatcttc acccttgttc cctggctccg ttacgtcccc ggaaggttcc
     2281 ccgcatgtac gcgcagaaga aatcgatcga aaactcccac atcgttcgcg agcgtgaccg
     2341 caagcgtttc cgcgagctgc ttgccgtcct cggtttagga gtcccgatcg gacttttcct
     2401 gctgctgttc acctggcaga acctcgaggt gattcgtctc ggacacgaag cgacgcgcct
     2461 gcagaagaag aaacaggaga tcgagaacgc gaaccgtgcg ctgcaactcg agctcgatcg
     2521 ccgcacctcg ctcggggcgg ttgaacagcg cgccaccgag ctcggttttc aacacaccga
     2581 cccgcgcgtc gtcgtaatgg tccagacacc gccggcggat tctgctccca ctccccaccc
     2641 cccacaaccc acccccgcag gttcccgctg atgcccatct cgcaaaagcg cctcctgcaa
     2701 ctcttcgcgt tcctcgccgc atgggcgctg gtcgtgatcg ttcgtctggc gcaagtccag
     2761 ctcgtgcgtc acggcgacta cgtcgtcaaa gcgcagcgac agcaggagag gacgctggcg
     2821 ctccagcccg tgcgcggctc gatcttcgac agccgccagc gcgttctcgc ggagagcgtt
     2881 gctgccgaat cgatctacgc cgacccgcaa gccatcggcg accgcgcgca gaccgcgaag
     2941 gcgttggcaa gcgttgccgc gctgcaactc agtgcggcgg agctgaatac caagcttgca
     3001 tccagcggcg gtttcgtctg gatcgcgcgc caactgccgc tcgaagtcag cgccgcggtg
     3061 aaagccctcc gcctgcccgg catctactat ctcgaagagc atcgccgctc c
//
LOCUS       DATLVW010000630         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_153234, whole genome shotgun sequence.
ACCESSION   DATLVW010000630 DATLVW010000000
VERSION     DATLVW010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153234"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VNN25_18500"
     CDS             complement(<1..1109)
                     /locus_tag="VNN25_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93579.1"
                     /translation="MADDYTTDETNVDTDFDASEISPQSPSIAGDYTAEDIKVLKGLD
                     AVRKRPGMYIGDTDDITGLHHMVYEVVDNAIDEALAGYANKVLVTIHTDNSVTVEDNG
                     RGIPVDLHKEENRSAAEVIMTELHAGGKFDNNSYKVSGGLHGVGVSVVNFLSEWLELE
                     IHRDDYVWRQRYEGGVPKKPIARGEKTTQRGTRVTFKADATIFSISEMNFETLSQRLR
                     ELAFLNSGVFIAISDDRTEKKHEFCYQGGIVSFVKDLNKNKVPLHEDPIFIRDEKDGE
                     AVEIAMQWNEGYTENLYCFTNTIKNLDGGTHLVGFKAALTRTMNKYIEASGILQKLKL
                     SLEGDDIREGLSAVISVKIRDPKFSSQTKDKLVSS"
     gene            complement(1237..2307)
                     /gene="recF"
                     /locus_tag="VNN25_18505"
     CDS             complement(1237..2307)
                     /gene="recF"
                     /locus_tag="VNN25_18505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXH93580.1"
                     /translation="MILRSIATQAFRNLASEEVAFHEHVNIVVGRNGQGKTNLLEAIY
                     FLATTKSFRTSRVASLFAFGSPSTFVSGQMHRFGVDKTLSVGLETGETRRRALLINGE
                     RVPLAVYVNAMSVFAYSSARLDILRGAPEERRRFLDRGIASIDPGYLEAVSRYGRVLR
                     QRNALLASGSASSLAAWDAELIASAAPLQRGRAAYTEALASSFAAIVAEHGYHIHNLA
                     IAYKPSSVDDLARIRRDELRARMSLVGPQRDVLEFSVNGRPAAEVLSGGEQKMVVLFL
                     KFAKLDLFRRRFDEPAVFLLDDVDAELDLEILEQLLVKLPSRTQVFATSAKERFLRTL
                     EAGPHRRLVIENGRVAATNDLP"
BASE COUNT          450 a          690 c          794 g          532 t
ORIGIN      
        1 tgggacgaga cgagcttgtc tttcgtctgc gatgagaact tcggatcgcg gatcttgacg
       61 gagatgacgg cgctaagtcc ttcgcggatg tcgtcgccct cgaggctgag cttcagcttc
      121 tgcaggatgc ccgacgcctc gatgtacttg ttcatcgtgc gcgtcagcgc ggccttgaag
      181 ccgacgaggt gcgtgccgcc gtcgaggttc ttgatcgtgt tcgtgaagca gtagaggttc
      241 tcggtgtagc cctcgttcca ctgcatcgcg atttcgaccg cttcgccgtc cttctcatcg
      301 cggatgaaga tcgggtcttc atgcagcggc accttgttct tgttgaggtc cttgacgaac
      361 gagacgatgc cgccctggta acagaactcg tgtttcttct ccgtgcggtc gtcgctgatg
      421 gcgatgaaga cgccggagtt gaggaaggcc agctcgcgaa ggcgctgcga gagggtctcg
      481 aagttcatct ccgagatcga gaagattgtt gcgtcggctt tgaacgtgac acgtgtgccg
      541 cgctgcgtgg ttttttcgcc gcgagcgatc ggcttcttcg gcacgccgcc ttcgtagcgc
      601 tggcgccaga catagtcatc gcgatgaatc tccagctcca gccattcgga gaggaagttc
      661 acgaccgaga cgccgacgcc gtgcagaccg ccggagactt tgtaggagtt gttgtcgaac
      721 tttccgccgg catgcagctc ggtcatgatg acttccgcgg cggagcggtt ctcttctttg
      781 tgcagatcga ccgggatacc ccggccgttg tcttcgacgg tcaccgagtt gtcggtgtgg
      841 atcgtgacga ggactttgtt cgcgtaaccg gccagcgctt cgtcgatcgc gttgtcgacg
      901 acttcgtaga ccatgtgatg caggccggtg atgtcatcgg tatcgccgat gtacatgccc
      961 ggacgcttgc gtacggcgtc gagacctttg aggactttga tgtcttccgc ggtgtagtcg
     1021 cccgcgatag aagggctctg cgggctgatt tcagacgcgt caaagtcggt gtcaacattc
     1081 gtctcgtctg tggtgtagtc gtctgccatt caattgcttt ccgggtgctg gtttcagggg
     1141 taggtagcgc gtctcccgac gagcttttaa cgcgaggatt gcgccgcgtt taagaacgct
     1201 gattgtatcg cgatttattg cgttccacgg aacgtttcac ggcaaatcgt tcgtcgccgc
     1261 aactcgcccg ttttcaatga ctaagcggcg gtgcggacct gcctccaacg ttcgcagaaa
     1321 acgctctttg gcagaggtgg caaaaacctg tgtccgggag ggtagtttaa cgagcagttg
     1381 ttcgaggatt tcaaggtcga gctcggcatc gacgtcgtcg agtaggaaga ccgccggctc
     1441 gtcaaaacga cgccggaaaa ggtcgagttt ggcgaacttc aggaagagga ccaccatctt
     1501 ctgctcgcca cccgagagca cttcggctgc cggtctgcca ttcaccgaaa actcgagcac
     1561 gtcgcgctgc ggcccgacca gcgacatgcg agcgcgaagc tcgtcacggc ggatccgggc
     1621 cagatcgtcg accgagcttg gcttgtacgc gattgcgagg ttatggatgt gatagccatg
     1681 ctcggcgacg atcgctgcga acgacgacgc cagcgcttcc gtgtaagcgg cacgtccgcg
     1741 ctggagtggg gccgccgagg caatgagctc cgcatcccag gcagcgaggg aagatgcgct
     1801 gccgctggcc aggagcgcgt tgcgttgacg gaggacacgt ccgtaacgac tgacggcttc
     1861 gagatagcca gggtcgatgc tggcgatacc gcgatcgaga aaacggcgcc gctcttccgg
     1921 cgcgccacgc aggatatcga gccgcgcaga cgagtaggca aagacgctca tggcgttgac
     1981 gtagaccgcc agagggaccc gctcgccgtt gatcagcagc gcgcgacgcc tggtctcccc
     2041 cgtctccagg ccgacggata gcgttttatc gacgccgaag cggtgcatct gcccggagac
     2101 aaaggtgctc ggcgagccga acgcgaagag gctggccaca cgcgaggtgc gaaacgattt
     2161 cgtcgttgcg aggaagtaga ttgcttcgag caggttggtt ttgccctgcc cgttgcggcc
     2221 gacgacgatg ttgacgtgct catgaaacgc aacctcttcg gatgcgaggt tgcggaacgc
     2281 ctgggtggcg atcgagcgga ggatcaagca gtcaaacgca tcggcatgac tacgtacagg
     2341 tattcgtatg cgatgtccgc gtcgttcgct gacggacggc cgatgcattg gctgttctcg
     2401 tccttcagat cgagcgagac ctgctgcgtg tcgaccgcgc tgaggaaatc ggtgacgtac
     2461 gccgcg
//
LOCUS       DATLVW010000631         5153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_562853, whole genome shotgun sequence.
ACCESSION   DATLVW010000631 DATLVW010000000
VERSION     DATLVW010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562853"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VNN25_18510"
     CDS             complement(<1..717)
                     /locus_tag="VNN25_18510"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH93581.1"
                     /translation="MRRLLIASALFLFAATLHAQCADVSTITGQLDGARMKQTVEKLV
                     SFGTRHTLSDTTSPTRGIGAARKWIFDEMTRIAATSSGRMTVAYQSSMQKSARTKNEP
                     VEMINVVATIKGTTDPDRIYIATGHYDSINSDNSNATLDAPGADDDASGVAVIMELAR
                     VLSQHPLEATVMLAAVQGEEQGLLGSRGLAEEAVAKKWNVEGMITNDIVGGAVGGEGD
                     IDNHTVRIFSANPKGAGEGSA"
     gene            768..3899
                     /locus_tag="VNN25_18515"
     CDS             768..3899
                     /locus_tag="VNN25_18515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH93582.1"
                     /translation="MSLRRGVLVLLLISVPVAARVLPSGVRPFRSYGTEAGLGNLAAM
                     RLAQDSAGFLWVATQDGVYRYDANRFVRFGLDEGLPSSYVQNVQAAPDGSVWVNTYGG
                     LARFDGRRFVAIPSSKSPINAFSIDQQNNVWIATADGILRGDATHPFARVAGWPDGEA
                     TTIWCGRGSGEPGVGSRSESPNATLNGTVPAFSPTPDPRSATPAHATLSATAPASSPT
                     PDSRPPTPSVWSSSRDHLGLLVNGVWTFWTNPADERIDAIVVDRNHTVWARSAAHLWS
                     KNEGDAAFHDESAALPATSTSGYLALDRRGNLWVPTDRGVAVHDENGWRVIGAADGLP
                     IEWSRDVLEDREGSIWIASLGVHRMLGRGELTTYDRRAGLPNAVTWCFVWDREQHLLV
                     GTDLGLARSTPAGWETVAGTAGHQIRSVVEEADGTLWAAGSPAEVLRVGRDGTVRKYA
                     VAGRTVLKIVRDRDGALWVATRGGGLLRKGANEDRFALIPVPNGPPDETFRWITLDNA
                     GRIWAAGEHGIACLDGGAWRRYTTRDGLAHDHAAYIIQSASGDFWAAYFEPLGIVRFS
                     IDPNKAMHVVDHLDLRRGLATNKVFMIGEDARRRLWVGTGVGVDLVTNSAKGPLIEHL
                     GMADGLSGDDVDAMAFLAGPDGETFIGTSSGFSHYVPRAGAPGIQPPPVRITASSVGA
                     KHDFSAAFAALTYLKQDLIEYQVRLLGLEEEWRPAVSREVRYAPLAPGQYRFETRARL
                     RPGAWSAPAGVTFTIAPAWWQTSSAQVLALLAVAALILLGHRMRVALLRRRNRELEAI
                     VAQRTSELADVNTRLLNLSVTDALTGMKNRRYLDYCMPEYMADALRRHESLTRSGLDP
                     TRANADLLFFLLDLDRFKDINDRFGHAAGDRVLVDIHELLKTVMRDTDTLVRWGGEEF
                     LLVARNSSRHEAAAIAERIRASIASHAFVVGTRTTSLRLTCSVGFAPFPFLPEDRLRF
                     GWPEVLDVADTCLYAAKNAGRNRCAGVISGDCRDPETLLQRIRKSLDDVARSGEVTVV
                     TSGGDVSAIRMAR"
     gene            complement(3980..4936)
                     /locus_tag="VNN25_18520"
     CDS             complement(3980..4936)
                     /locus_tag="VNN25_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93583.1"
                     /translation="MKRGCFVTLLILVALIGGYWYLLHGHIEPPALWWSVGIASFFMW
                     ISTGALRTAAMAARDASRVASQSSFGGFSGEEMTDGETVTIVGRVRALGSPLRAPFSD
                     QPAVLYSYEIEHVTQNREGTSTAKDYSGFALAPTAIDSSRGSINLLGFPLLEGFPKTT
                     IDTDAARQNAAAYVASTAFTDMQGFHPGQIYHQIRDVLTDDDGHLRKDWKMTEDRDLS
                     GKTLTEQIVAPGEQVCVIGSWSAAKHGLVPGGTDGVIQLLRGDPQKVGSNLWGKCIRN
                     VIGGLIVAAIVNGGVYVLLRAAAGDPRFSFLSQPIFGRSNKN"
BASE COUNT          849 a         1783 c         1598 g          923 t
ORIGIN      
        1 cgccgatcct tcgccggcgc ctttcggatt cgccgaaaag attcgcacgg tgtgattgtc
       61 gatgtcgcct tcgccaccga ctgcgccgcc gacgatgtcg ttcgtgatca tcccctcgac
      121 gttccacttc ttcgcgaccg cttcttcggc cagccctcgc gatccgagca gtccctgttc
      181 ttcgccctga accgcggcca gcatcaccgt ggcctcgagc ggatgctgcg agagcacgcg
      241 ggccagctcc atgatcacgg caacgccgga tgcatcgtcg tcagcgcccg gtgcgtcgag
      301 cgtcgcgttc gagttgtcgg agttgatcga atcgtagtga ccggtggcga tgtagatacg
      361 atcgggatca gtcgtgccct tgatcgtggc caccacgttg atcatctcca ccggctcgtt
      421 cttcgtgcgc gcgctcttct gcatcgacga ctgatacgcc accgtcatcc ggccgctgct
      481 cgtcgcagcg atgcgcgtca tctcgtcgaa gatccacttg cgcgccgcgc cgattccgcg
      541 cgttggcgac gtcgtatccg agagcgtgtg ccgcgtgccg aaggagacga gcttctcgac
      601 tgtctgcttc atccgggctc cgtcgagctg tccggtaatt gtggaaacat cggcgcactg
      661 ggcgtgcagg gtggcggcga agagaaaaag ggcggaggcg atcagtagtc tgcgcatact
      721 gaacatcgta cctccgcgcc gaaacagcgc taccatcatc gtctcccatg tcccttcggc
      781 gcggcgtgct cgttctcctg ctgatctccg ttccggttgc ggcgcgagtc ttaccgtccg
      841 gagtacgccc gttccgcagc tacggcaccg aggcgggact cggcaatctc gcagcgatgc
      901 ggctggcgca ggattccgcc ggcttcctct gggtggccac gcaggacggc gtctatcgat
      961 acgacgccaa ccgcttcgtc cgcttcggac tcgacgaagg actgccgtcg agctacgtgc
     1021 agaacgtgca ggcagcgcct gacggcagcg tctgggtcaa cacctacggc ggcctggccc
     1081 gattcgacgg acgacgattc gtcgccattc cctcatcaaa gagtccgatc aacgcgttct
     1141 cgatcgacca acagaacaac gtctggatag cgaccgccga tggcattctg cgcggcgacg
     1201 ccacgcatcc attcgcgcgt gtcgccggat ggccggatgg cgaagcgacg acgatctggt
     1261 gcgggcgggg gagtggggag ccgggggtcg ggagtcggag cgaaagcccg aatgcgaccc
     1321 tgaacgggac agtccccgcc ttttctccga cccccgaccc ccgatctgcg acccccgcgc
     1381 atgcaactct gagcgcgact gcgcccgcct cttctccgac ccccgactcc cgacccccca
     1441 ccccctccgt ctggtcctcc tctcgcgacc acctcggcct cctcgtcaac ggcgtctgga
     1501 ccttctggac caacccggct gacgagcgca tcgacgccat cgtcgtcgac cgcaaccaca
     1561 ccgtctgggc gcgcagcgcg gcgcatctct ggtcgaagaa cgagggggac gccgcgttcc
     1621 acgatgagag cgccgcgttg ccggccacga gcaccagcgg ctatctcgct ctcgaccggc
     1681 gcggcaatct ctgggttcca accgatcgcg gcgtggccgt acacgatgag aacggctggc
     1741 gtgtcatcgg tgcggcggac ggcttgccga tcgaatggtc gcgcgacgtt ctcgaagacc
     1801 gcgagggctc gatctggatt gccagcctcg gcgtccaccg catgctcggc cgcggcgagc
     1861 tgacgacgta cgatcgccgg gcaggtctgc cgaatgccgt cacgtggtgt ttcgtgtggg
     1921 accgcgagca acatcttctc gtcggcacgg acctcggcct tgcgcgctcg acgccagccg
     1981 gttgggagac cgtcgccggc accgccggcc atcagatacg cagcgtcgtc gaagaagccg
     2041 atggcacgct ctgggccgcc ggaagtccgg ccgaggtgct acgagtcggg cgcgacggca
     2101 ccgtgcgcaa gtacgcggtc gccggcagaa ccgtcttgaa gatcgttcgc gatcgcgacg
     2161 gcgcgctctg ggtggccacg cgcggcggcg gccttttacg caaaggcgcg aacgaggatc
     2221 gcttcgcgct catcccagtt cccaacgggc ctcccgacga gacctttcgc tggatcacgc
     2281 tcgacaacgc cggccgcatt tgggcggcgg gcgagcacgg catcgcctgc ctcgatggtg
     2341 gcgcctggcg gcgctacacg acccgcgacg gactggcaca cgaccacgcg gcctacatca
     2401 ttcagagcgc atcgggcgat ttctgggctg cctacttcga gcccctcggc atcgttcgct
     2461 tttcgatcga cccaaacaag gcgatgcacg tcgttgatca tctcgatctt cgccgcggac
     2521 ttgccacaaa caaggtgttc atgatcggcg aggatgcgcg gcggcggctg tgggtcggca
     2581 ccggagtcgg cgtcgacctc gtcaccaatt ccgcgaaggg cccgctcatc gagcacctcg
     2641 gcatggccga cggcttgtcc ggcgacgacg tcgatgcgat ggcttttctg gctgggcccg
     2701 acggagagac attcatcggc accagctccg gattcagcca ctacgttccg cgtgccggcg
     2761 cgccgggcat ccaaccgccg cccgtgcgca tcacagcctc ctcggtcggt gcgaagcacg
     2821 acttctccgc ggccttcgcc gcgctgacgt acctcaaaca ggatctcatc gagtaccagg
     2881 tgcgcctgct cgggctcgaa gaagaatggc gccccgccgt ctcgcgcgaa gtgcgttacg
     2941 cgccgctcgc gcccggccag tatcgtttcg agacgcgcgc acgtctgcgg ccgggagcgt
     3001 ggagcgcgcc ggcgggagtg acgttcacga tcgcgcctgc gtggtggcaa acgtcctcgg
     3061 cgcaagtcct cgctctgctc gccgtcgccg cgttgatcct cctcgggcat cgcatgcgcg
     3121 tcgcgcttct gcgccggcgc aatcgcgagc tcgaagcaat cgtggctcag cgcaccagcg
     3181 agctggccga cgtcaacacc cgcctgctca acctcagcgt caccgatgcg ctcaccggca
     3241 tgaagaaccg gcgctatctc gactactgca tgcccgagta catggccgat gccctgcgac
     3301 gccatgagtc gctcacgcgc tcgggcctcg atccgacgcg cgccaacgcc gacctcctct
     3361 tcttcctcct cgatctcgac cgcttcaaag acattaacga ccgcttcggc cacgccgcgg
     3421 gggatcgcgt gctcgtcgat atccacgagc tcctgaaaac ggtcatgcgc gacaccgaca
     3481 cgctcgttcg ctggggcggc gaggagtttc tgctcgtggc ccgcaactcc tcgcgccatg
     3541 aagcggccgc gatcgccgaa cggattcgtg cttcgatcgc gagccacgcc ttcgtcgtcg
     3601 gcacgcgcac gacgtcgttg cggctcacct gctcggtcgg gttcgcgccg ttcccgttcc
     3661 tgccggagga ccggctccgc tttggctggc ccgaggtgct ggatgtggcc gacacgtgcc
     3721 tctatgccgc caagaacgcc ggccgcaacc gctgcgccgg cgtcatctcc ggcgattgcc
     3781 gcgatcccga aaccctgctg cagcgcatcc gcaaatctct cgatgatgtg gcccgctcgg
     3841 gcgaagtgac ggttgtgacg agcggcggcg acgtgagcgc gatcaggatg gcgagatagc
     3901 taaaaacctg tctaaccaca gagacacaga ggtcacagag taacggttac aaactctgtg
     3961 tcctcagtgt ctctgtggtt cagtttttgt tcgacctacc aaagataggc tgactcagga
     4021 acgagaagcg cggatcacca gccgctgcgc ggagcagcac gtaaactccg ccgttgacga
     4081 tcgcggcaac gatcagtcct ccgatcacgt tgcggatgca cttgccccac aggttgctcc
     4141 cgaccttctg cggatcgccg cgcaggagtt ggatgacgcc gtctgtgccg cccggcacca
     4201 gaccgtgttt cgccgccgac caactgccga tgacgcaaac ctgctcgccc ggtgcgacga
     4261 tctgctcggt cagcgtcttg cccgaaagat cgcggtcttc ggtcatcttc cagtccttgc
     4321 ggaggtgtcc gtcatcgtcg gtcagcacgt cgcggatctg gtggtagatc tggccgggat
     4381 ggaagccctg catgtcggtg aaggccgtcg acgcgacgta tgccgcggcg ttctgccgcg
     4441 ccgcatccgt gtcgatcgtc gtcttcggaa agccttccag caacggaaac ccgaggaggt
     4501 tgatcgatcc gcgcgaggaa tcgatggcgg tcggagccag cgcaaagccg gagtaatcct
     4561 tcgcagtgct cgtgccctcc cggttctgcg tgacgtgctc gatctcgtag ctgtacaaca
     4621 ctgccggctg atcgctgaac ggagcgcgca gcggcgagcc gagcgcgcgc acacggccga
     4681 cgatcgtgac cgtctcgccg tccgtcatct cctcgccgct gaatcccccg aacgacgact
     4741 ggctcgcaac gcgcgacgca tcccgcgcgg ccatcgccgc cgtccgcagc gcccccgtcg
     4801 agatccacat gaaaaagctg gcaatgccca cgctccacca gagcgccggc ggctcgatat
     4861 ggccatgcag cagataccaa tagccgccga tcaacgcgac gaggatcagg agcgtgacga
     4921 aacacccgcg cttcatgagg gcaatgctaa cgcgaccggg atgtcgaacg agtcacttcc
     4981 gctccggaat ctccttcccg ttcttcgcct tcatcgaatc ctcctgaagg gccgggcgcg
     5041 ccgcgatcgg cgcgcccggc ttactgcatg actacatcac tgctcagacg tgggcgttgg
     5101 cgcaggtgcc ggcggaactt tcggctgctg gttgcgtccg aagcggtttg cct
//
LOCUS       DATLVW010000632         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_316753, whole genome shotgun sequence.
ACCESSION   DATLVW010000632 DATLVW010000000
VERSION     DATLVW010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316753"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VNN25_18525"
     CDS             <1..788
                     /locus_tag="VNN25_18525"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HXH93584.1"
                     /translation="HIRAAWSYINQEGNAAKYSPENLAKVKAKIRAAMKRIGADVADS
                     SGGKPSAIDQGDDDETKENIVAEEQPVTDYLKALGLPADADPKHKLATAFRDKDEKIL
                     ALTQEITELTAASIEKDGKASELASRVAELESKDRARDIDVILARAVEKGRVLPAEKE
                     TLAEIFAADVDGLRKMIATRPAEMFALEAKGTGGGDTDRFVDDPDVAQFVKGMNTGGD
                     PVDTEQAKQHLMALDILREQGKAETYTNDEYVAAYNKAAPLVY"
     gene            798..1196
                     /locus_tag="VNN25_18530"
     CDS             798..1196
                     /locus_tag="VNN25_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93585.1"
                     /translation="MAHETQAESYIFSGRAASAIQPYGVLSFPVGSGIDYGLVPAAAG
                     ASGVSFGVSRASHAVGDAAECITYGEAKVRALASLGAGAIVGAGAAGSIGVLPVAAPS
                     GPTVTSSVGVAVQNAVLNDIFTILVRPLSN"
     gene            1241..2242
                     /locus_tag="VNN25_18535"
     CDS             1241..2242
                     /locus_tag="VNN25_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93586.1"
                     /translation="MATTSVGNVHVNVPLSNLAIRYMPLMDGFIADEVCPRIPVVNES
                     NVYYTWQQADFFSVDVSDLVPDRGTPREIDFTATTASYSANRRELGWTISQRERNNAD
                     NQLDLETAKQQGVLARLALLRDQRVAALLKAGGTTTVLGEIITGGLDSSMTAAANPKF
                     DVSTTLWSDFTGQVTVGIRAMRQTIGVRPNTLIIPAYVAEGMQKSGIFTAAGGPINQY
                     TGTPADNAYFDQYPLLPTSIMGMRVLVPGNIKNTAKEGQTASYSDVWGKDVTLLYVTP
                     GPSMTVPSVSYTFQAEPLLTRQARDDVRRMDEYFLGNTIAEKVVAPFAGYTITAAAT"
     gene            2389..>2590
                     /locus_tag="VNN25_18540"
     CDS             2389..>2590
                     /locus_tag="VNN25_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93587.1"
                     /translation="MSALKQRHPIPEPRIAACFITKNSEATLEAAIASVRPFVHEINI
                     YDTGSKDGTFDLLDKLGKEKLLV"
BASE COUNT          504 a          872 c          808 g          406 t
ORIGIN      
        1 cgcatattcg tgccgcgtgg agctacatca accaggaagg caacgctgcc aagtattcgc
       61 cggagaacct tgcaaaggtg aaggcgaaga tccgggcggc gatgaagcgg atcggtgctg
      121 atgtggccga ttcgtcaggc ggcaagccct ccgccataga tcagggcgac gacgacgaaa
      181 cgaaggagaa catcgtggct gaagagcagc cggtcaccga ctacctgaag gcgctcgggc
      241 ttcctgcgga tgcagatccc aagcacaagc tcgcgaccgc cttccgcgac aaggacgaga
      301 agatcctcgc gctcacccag gagatcacag aattgactgc ggcgagcatc gagaaggacg
      361 gcaaggcatc agagttggcc tcgcgtgtcg ccgagctgga gtcgaaggac cgcgcccgcg
      421 acatcgatgt catcctggcc cgtgccgtcg agaaggggcg ggttctgccc gcagagaagg
      481 aaacgctcgc cgagatcttc gcggcggacg tggacgggtt gcggaagatg atcgcgacgc
      541 ggcccgcaga gatgttcgcc ctcgaggcga agggaacggg aggcggcgac accgaccgct
      601 tcgtcgacga ccccgacgtg gcccagttcg tcaagggcat gaacaccggc ggcgaccctg
      661 tcgacaccga gcaggccaag cagcacctca tggccctgga catcctccgg gagcagggca
      721 aggccgagac ctacaccaac gacgagtacg tggcggcgta caacaaggcc gcgccgctcg
      781 tctactagga gacactcatg gcacacgaaa cccaggccga gtcctacatc ttcagtggaa
      841 gagcagcctc ggcaatccag ccctacgggg tgcttagctt cccggtcggg tccggtatcg
      901 attacgggct tgtccctgcg gcggcgggcg ccagcggcgt ctcgttcggc gtcagccgcg
      961 catcgcacgc ggtcggggac gccgccgagt gcatcaccta cggtgaggcg aaagtgcggg
     1021 cgctggcatc actcggcgcc ggggcaatcg tcggagccgg cgcagcggga tcgatcgggg
     1081 tgctcccggt cgccgcaccg tccggcccga cagtcaccag ctcggtaggt gtggctgtcc
     1141 agaacgcggt gctgaacgac atcttcacga tcctcgttcg gccactgtcg aactaaatcc
     1201 cccacccggc gagtcgcctc gccccttcta aggagcagta atggcaacca cctccgtggg
     1261 gaatgtccat gtcaacgttc ccctctcaaa cctcgcgatc cggtacatgc cattgatgga
     1321 cggcttcatc gccgacgagg tgtgtccccg gatccccgtg gtcaacgaat cgaacgtcta
     1381 ctacacctgg cagcaggccg acttcttctc ggtcgatgtc agcgacctcg tgcccgatcg
     1441 tggaacgccg cgcgagatcg acttcacagc aacgaccgct tcctatagcg cgaaccggcg
     1501 cgagctcggc tggacgatct cccagcgcga gcgcaacaac gccgacaacc agctcgacct
     1561 cgagacggcg aagcagcagg gtgtcctcgc ccgtcttgcg ttgcttcgcg accagcgcgt
     1621 tgccgccctg ttgaaggcgg gcggcaccac gaccgtcctc ggcgagatca tcacgggcgg
     1681 cctcgattcg tcgatgaccg ctgcagcgaa cccgaagttc gacgtctcga cgactctctg
     1741 gtcggacttc accggccagg tgacggtcgg gatccgggcg atgcggcaga cgatcggcgt
     1801 ccgcccgaac acgctcatca tccccgccta cgtcgccgag ggaatgcaga agtccggcat
     1861 cttcacggca gccggcggcc cgatcaacca gtacaccggc acgcccgccg acaacgcgta
     1921 cttcgaccag tacccgctcc tcccgacctc gatcatgggg atgcgtgtcc tggtgccggg
     1981 caacatcaag aacacggcga aggaaggcca gacggcctcc tactcggacg tgtgggggaa
     2041 ggacgtcacc ctcctctacg tgacgcctgg cccgtcgatg acggtcccgt cggtctcgta
     2101 cacgttccag gctgagccgc tgttgacgcg gcaggctcgg gatgacgtgc gccggatgga
     2161 cgaatacttc ctcggcaaca cgatcgccga gaaggtcgtc gccccgttcg ccgggtacac
     2221 gatcaccgcg gccgctacct agaccgcagc ctgacagaga agggcgagga gccccgccct
     2281 tctcgtttcc cctcggcagg agagccaggg ggtcctcttc gcgtcgggct cccgttgctg
     2341 gggtccccct ggctgtcctg tcgttcactg atccgcaagg agcccgcttt gtctgctctc
     2401 aagcagcgcc acccgatccc cgaaccacgc atcgccgcct gcttcatcac caagaactcg
     2461 gaggcgacac tcgaagccgc gatcgcctcg gtgaggccgt tcgtccacga gatcaacatc
     2521 tacgacaccg gctccaaaga cggcacattc gacctgctcg acaagctcgg caaggagaag
     2581 ctgcttgtcg
//
LOCUS       DATLVW010000633         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_316947, whole genome shotgun sequence.
ACCESSION   DATLVW010000633 DATLVW010000000
VERSION     DATLVW010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316947"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VNN25_18545"
     CDS             <1..297
                     /locus_tag="VNN25_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93588.1"
                     /translation="TKSKTINVPLAKLYAAFSNARTRAKWLGGVKLTVRGATPNKTIR
                     LRWEDGAPIDIGFYAKGESKSQVALEHRKLATKADADRIRAFWTDRLNALAAML"
     gene            complement(348..1658)
                     /locus_tag="VNN25_18550"
     CDS             complement(348..1658)
                     /locus_tag="VNN25_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH93589.1"
                     /translation="MNRITRALRHRNYRLFFGGQSISLIGTWITRIATSWLVYRLTGS
                     ELLLGVVAFCSQIPLLILAPIAGVLVDRWDRHRILVITQILSALQSAALAMLAFTHVI
                     TVAEVIVLQLVQGVINSFDTPARQAFVVEMVADREDLPNAIALNSSMINASRIIGPSI
                     GGVLIATVGEGWCFGVDAISYIAVIISLLMMQLTLAPRARVDTHMLDELRTGWRYVAG
                     FAPVRALLLIIALVGVMGMPYATLMPVIASRVLHGGPHTLGILMTASGIGALMGTVYL
                     ASRHSVLGLGKVIVAANAALSLGLMAFSFSRSLWLSAILLPVVGAGIMVQAASVNTIL
                     QTVVDENLRGRVMAFYSVAIMGMQPIGSLLSGVVAERIGAEMTIFYGALFCLIGGIWF
                     AMRRAMLAEHVRPIYIERGILPWDDGNLAPAPAPGVEIRISDDQ"
     gene            1789..1864
                     /locus_tag="VNN25_18555"
     tRNA            1789..1864
                     /locus_tag="VNN25_18555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1822..1824,aa:Ala,seq:ggc)
     gene            complement(1927..>2892)
                     /locus_tag="VNN25_18560"
     CDS             complement(1927..>2892)
                     /locus_tag="VNN25_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93590.1"
                     /translation="SRLPREAVAAALYESEPLLARLGSAWTWQLTLALLGPAEASFSV
                     IGLFIARSDDPPARTLLAGRPIPFIRRLTADQGARDAIRGKPPHLDALLNDPRGHLLF
                     DFEANALLVAAAFVDASLALRLADSLCESGKPSAAALTVVARRAVAAGLAEEVAARAP
                     TYLDWTQAAAFAGALTPEAVEAIVTSLAPRVVDDADPDQKHVGAVPLLAALAVTGRFD
                     TLASLARSATIPPLSVVQLLLNLNCRSALDALSRSETFIMWSRATHDAYAGTLGGGPR
                     LLPDSAGEEASSAAVLDALLVSAAAPWAVVPGSFEDELDIPQPAL"
BASE COUNT          590 a          936 c          940 g          426 t
ORIGIN      
        1 accaaaagca agacgatcaa cgttccgctg gcgaagctct acgccgcctt cagcaacgcc
       61 cgaacccgcg cgaagtggct gggtggcgtg aagctgaccg ttcgcggcgc caccccaaac
      121 aagacgatcc gcctccgctg ggaagacggc gccccgatcg acatcggctt ctacgcaaaa
      181 ggtgagtcga aaagccaggt cgccctcgag caccgcaaac tcgccacgaa agccgacgcc
      241 gaccgaatcc gcgccttctg gaccgaccgc ctgaacgcct tagcagcgat gctctaggag
      301 tgcgggcgtc tcgcccgcgg ccaccggggc gtccccgccc cggtggttta ttgatcatcc
      361 gagattcgaa tctccactcc cggcgccggc gccggagcca ggttcccgtc atcccacggc
      421 aaaatccccc gctcgatata aatcggccga acatgctccg ccagcatggc ccgtcgcatc
      481 gcgaaccaga ttccgccgat caggcagaac agcgccccgt agaagatcgt catctctgcg
      541 ccaatgcgct cggccacgac tcccgacagc agacttccga tcggctgcat ccccatgatg
      601 gccaccgaat agaacgccat cacccgtccg cgcaggttct catcgaccac cgtctgcagg
      661 attgtgttca ccgacgccgc ctgcaccatg attcccgcgc cgaccaccgg cagcaaaatc
      721 gccgacagcc acagcgagcg cgaaaacgag aacgccatca gcccgaggct gagagcggcg
      781 ttggcggcga cgatgacctt gccgaggccg aggacggagt gccgggaggc gaggtagacc
      841 gttcccatca gcgcgccgat gccggacgcc gtcatcagaa tgccgagggt atgcggcccg
      901 ccgtgcagaa ccctcgaggc gatgaccggc attagcgtcg cgtacggcat tcccatcacc
      961 ccgaccaggg caatgatcag cagcagcgcg cgcaccggcg cgaagccggc tacgtacctc
     1021 catcccgtcc gcagctcgtc gagcatgtgc gtgtcgacgc gtgcgcgcgg cgcaagcgtg
     1081 agctgcatca tcagcaacga gatgatcacg gcgatgtagg agatggcgtc gacgccgaag
     1141 caccatcctt cgccgaccgt ggcgatgagc actcctccga tcgacggccc gatgatgcgg
     1201 gaggcgttga tcatcgacga gttgagcgcg atggcgttcg gcaggtcctc gcggtcggcg
     1261 accatctcca cgacgaacgc ctgccgcgca ggcgtatcga acgagttgat caccccttgc
     1321 accagttgca gcacgatcac ctcggccacc gtgatcacgt gcgtgaacgc cagcatcgcc
     1381 agcgcggccg actgcaacgc ggagaggatc tgcgtgatga cgaggatgcg atgccggtcc
     1441 caccggtcga cgagcacacc ggcgatggga gccaggatca gcagcggaat ctggctgcag
     1501 aacgcaacga cgccgagcag gagctccgag ccggtgaggc ggtaaaccag ccagctcgta
     1561 gcgatacgcg tgatccacgt accgatgagc gagatgctct gtccgccgaa gaagaggcgg
     1621 tagttgcggt gccggagtgc gcgggtaatc cgattcaagc gagcctctcc tcgcaattga
     1681 tgttcccacg cccctcggct agaatcgcgc ccccgggaaa tttcgacgcg cccgccgcgt
     1741 tagttcccgt accctgccgc agatagggca gccagccaga aacatcgtgg ggccgtagct
     1801 caattgggag agcgcctgaa tggcattcag gaggtcgacg gttcgatccc gttcggctcc
     1861 accaaatcaa caagatcacc aatttgcgcc gatccgagag ggccggcgtt ttcgttacgc
     1921 ctcagatcag agagcgggct gggggatgtc aagctcgtcc tcgaacgagc ccggcactac
     1981 tgcccaaggt gccgcggcag acacgagcaa agcatcgaga acggcggccg aactggcctc
     2041 ctcccctgcg ctgtccggca gcagacgggg accgccgccc agcgttcctg catacgcatc
     2101 gtgtgtcgca cgagaccaca tgatgaatgt ctcgctgcga gagagtgcgt cgagtgcgga
     2161 gcggcagttc agattgagca ggagttgaac cacggagaga ggtggaatcg ttgcggatcg
     2221 ggcgagggaa gcgagtgtgt cgaaacggcc ggttacggcc agtgcggcga gaaggggaac
     2281 ggcgccaacg tgtttttggt ccgggtctgc gtcatcgact acgcgcggag caagagacgt
     2341 gacgattgct tccacggctt caggagtcag cgcgccggca aaggcggcgg cctgcgtcca
     2401 atcgagatag gtgggggctc gggccgcgac ttcttcggcg agccccgccg ccactgcgcg
     2461 gcgcgcgacg actgtcaatg cggccgcact cggcttgccc gactcgcaga gtgaatcggc
     2521 aaggcgcaag gccagggacg catcgacgaa ggcggcggca acgagcagcg cgttcgcttc
     2581 gaagtcgaaa agcagatgac cccgcggatc gttcagtaga gcgtcgagat gcggaggttt
     2641 gccgcggatt gcgtcgcgcg cgccttgatc ggcggtcaat ctgcggatga aaggaatggg
     2701 tctcccggcc aacagtgtcc gggcgggagg gtcgtccgag cgcgcgataa acagtccgat
     2761 cacggaaaag gaggcttcgg cggggccgag gagcgcgagg gtcagctgcc aggtccaggc
     2821 cgagccaagg cgcgccagca acggctcgga ttcgtacaac gcggcggcaa cggcctcgcg
     2881 cgggaggcgt ga
//
LOCUS       DATLVW010000634         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_236724, whole genome shotgun sequence.
ACCESSION   DATLVW010000634 DATLVW010000000
VERSION     DATLVW010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236724"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VNN25_18565"
     CDS             complement(<1..975)
                     /locus_tag="VNN25_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXH93591.1"
                     /translation="MKSSPFPFVPLRAVLLACVLGFIGSAVALAQAAPPSGDAKSQEK
                     AAADKGPDKSALPPLPAPAHVPQQIMLDGKPLKYTVTVGTVQVRDKDGKTAGEVVTTA
                     YTVEGENRPVTFAFNGGPGASSVYLNFGALGPRHMNAGNEGDSPSDPVTLTDNQGTWL
                     DFTDLVFIDPIGTGFSRASVPEDQQKKLFFSTQSDIEYLSRVVYDWLVLNDRLASRKY
                     LVGESYGGFRGPRITHYLQSQLGVALNGVVLVSPYLNPSQDANGDMSPLPWMMTLPSI
                     TAAHLEREKKLTPEAMADVIAYTRGEYATTLLKGRSDEAGQQKMIQRVT"
     gene            1066..>2050
                     /locus_tag="VNN25_18570"
     CDS             1066..>2050
                     /locus_tag="VNN25_18570"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH93592.1"
                     /translation="MKHQLLATIFAAAFTAGAVAQQSGTPASQPAQELPDGPQPTTVQ
                     PNGPTVVMDTSMGQITCQFFQKQAPVAVANFIGLANGTKDWTDPATGKKMRDKPFFDG
                     TTFHRVIPEFMIQGGDPTGTGTGDPGYSFNDEFDPNLNFDVPGRLALANSGPNTNGSQ
                     FFITEQAYATLNQHYTIFGQCDEPSVNVVKTIARVQRDSNDKPLTPVTLNKVTIESEN
                     GAPPPQAGGGGSVVAEPPKRVNISAGVAVGMLESKTPPAYPAEAKQKGISGRVILDTI
                     IGTDGTVQELKVVSGPEGLQQAALDAVKSWRYRPYLLNGDPVEVRTTVNVIF"
BASE COUNT          416 a          577 c          693 g          364 t
ORIGIN      
        1 cgtgacgcgc tgaatcatct tctgttgccc agcctcgtcg ctgcggccct tgagcagcgt
       61 ggtggcgtat tcgccgcgcg tgtaggcgat gacgtcggcc atggcctcag gcgtgagttt
      121 cttttcgcgt tcaagatgcg cagccgtaat ggagggcaga gtcatcatcc acggcagagg
      181 cgacatgtcg ccgttggcat cctgcgacgg attcaggtaa ggcgagacga ggaccacgcc
      241 gttcaaggcc acgccgagct gcgactgcag ataatgcgtg atgcgcgggc cgcggaagcc
      301 accgtagctc tcgcctacca ggtatttgcg cgaggcgagc cgatcgttga gcacgagcca
      361 gtcgtagacc acgcgggaga ggtactcgat gtcggattgc gtggaaaaga agagcttctt
      421 ctgctgatcc tcggggacgc tggcgcggct aaagccggtt cctatgggat cgatgaagac
      481 gaggtcagtg aagtcgagcc aggtgccttg attgtcggtg agagtcacag ggtcggatgg
      541 gctgtcgccc tcgttgcccg cgttcatgtg ccgtggcccg agggcgccga agttgagata
      601 gacgctggac gcgccggggc ctccgttgaa tgcaaacgtg acgggccggt tttcgccttc
      661 gacggtgtag gcggtcgtga ccacctcgcc tgcggtcttg ccgtccttgt cgcgcacctg
      721 cacggtaccc accgtgacgg tgtacttgag cggcttgcca tcaagcatga tctgctgcgg
      781 aacgtgggcc ggcgcgggca gaggcggaag agcggattta tcggggcctt tatcggcagc
      841 ggccttttcc tgggacttgg catcgccgga gggaggcgcg gcctgcgcga gggcgacggc
      901 ggagccgatg aagccaagga cgcaggcaag caaaactgca cggagaggga cgaacgggaa
      961 cggggaactt ttcatgcaat taaagataca gggcgattgc cggtgagggg caggcatgag
     1021 aggcgtggca tgcggcaagc cctatcgttt aacctgaaga acggcatgaa acatcagctt
     1081 ttggcgacca tctttgcagc agcattcacg gccggcgctg tggcgcagca gtcgggcacg
     1141 ccggcttcgc agcccgcgca ggagctaccg gacggtcctc agcctacaac ggtccaaccg
     1201 aatggcccga cggtggtgat ggacacttcc atgggtcaga tcacctgcca gttctttcag
     1261 aagcaggcgc cggtggccgt ggcgaacttc atcgggctgg cgaacgggac gaaggactgg
     1321 accgatcccg cgaccggcaa aaagatgcgt gataagccct tcttcgacgg gaccacgttt
     1381 caccgcgtga tcccggagtt catgatccag ggcggcgacc cgacggggac cggaacgggc
     1441 gatccgggct acagcttcaa cgacgagttc gatccgaacc tgaacttcga cgtgccgggg
     1501 cggctggcgc tggctaactc cgggcccaac accaacggca gccagttctt catcacagag
     1561 caggcgtatg caaccctcaa tcagcactac accatctttg ggcagtgcga cgagccgagc
     1621 gtaaatgttg tgaagaccat cgcgcgcgtg cagcgtgact ccaatgacaa gccgctgacg
     1681 ccggtgactc tgaacaaggt gacgatcgaa tcggagaacg gagcacctcc accgcaggcg
     1741 ggtggtggcg gtagcgttgt ggcggagcct ccgaagaggg tgaacatctc ggcgggcgta
     1801 gccgtgggga tgctggaatc aaagactcca ccggcgtatc ccgcggaggc caagcagaag
     1861 ggcatttccg gacgagttat tctcgacacg attatcggaa ctgacggaac ggtgcaggaa
     1921 ctgaaagtgg tcagtggccc agagggactg caacaggccg ctctcgacgc agtgaaatcc
     1981 tggcgctacc ggccctacct gctgaacggc gatccggtgg aagtacggac cacggtcaat
     2041 gtgattttct
//
LOCUS       DATLVW010000635         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_361561, whole genome shotgun sequence.
ACCESSION   DATLVW010000635 DATLVW010000000
VERSION     DATLVW010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361561"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..574
                     /locus_tag="VNN25_18575"
     CDS             50..574
                     /locus_tag="VNN25_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXH93593.1"
                     /translation="MVAPAFAKPAAHADVAADPNAIPVALNVNGRVHELRLDPRTTLL
                     DALREHLNLTGTKKGCDQGACGACTVLVDGRRINSCLTLAVMNSGRKITTIEGIAANE
                     RLHPVQAAFIRNDGFQCGYCTSGQICSAVAVIQEGHAGSDDEIREWMSGNICRCGAYE
                     GIVAAVREASGRRA"
     gene            571..1557
                     /locus_tag="VNN25_18580"
     CDS             571..1557
                     /locus_tag="VNN25_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXH93594.1"
                     /translation="MNPFDFVRAREVSAALSSGSRADAKFIAGGTNLVDLMKCDVERP
                     AHLVDVNDLKLAGIERVSGGIRIGAMARMSDVARHPLVLQSAPAVSQALLASASPQIR
                     NAASIGGNLMQRTRCAYFRELTFAPCNKRNPGSGCAAIDGENRMHAVLGGSESCIATH
                     PSDLAVAVAALDAVLTLHGPNGDRHVAAADFHLLPGTTPQTEHDLKHGELITSVFIPD
                     APHAKRSAYLKIRDRAAYEFALASAAVGLEISGGTIHSARIALGGVATKPWRAKFAEA
                     ALIGQAPSKDVFRAAAEAEMKSAVAHGQNAYKIELAKRTLVRALGDLAGGAA"
     gene            1554..>2256
                     /locus_tag="VNN25_18585"
     CDS             1554..>2256
                     /locus_tag="VNN25_18585"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH93595.1"
                     /translation="MIPNIGKPLDRVDGRQKVTGGAHYATENKLDRVTYAALVDSTIP
                     AGTIKSIDDSRAKKAPGVLAVISHLNAPKLNPPPPPKPGGGGEQSSSLTESKYLPFSD
                     NTIHYLGQHIAVVVADTLEHAVHGAELVRVAYDVAPARTDIEKYRGEAKDRSQQSPSA
                     FTKGDADAALAGAAVKIDQIYRTPHEHHNPMEAHGLIAAWDGDHLTLHDSSQNIFAVQ
                     RTFATLFDIPKENVHV"
BASE COUNT          428 a          778 c          689 g          361 t
ORIGIN      
        1 gagccggcgc tcctttctga tcagttcggc gaccgtgacg gcgtcgctgc tggtcgcgcc
       61 tgccttcgcc aagccggcag ctcacgcgga tgtggctgcc gatccgaatg ccattccggt
      121 cgcgctcaac gtcaacggac gcgttcacga gcttcgcctc gatcccagga cgaccttgct
      181 cgacgcactt cgcgagcatc taaacctgac cggcacaaag aagggctgcg atcagggagc
      241 ttgcggcgcg tgcacggtgc tcgtcgacgg acgccgcatc aactcctgcc tcaccctcgc
      301 ggtcatgaac agcggcagaa agatcaccac gatcgaaggg atcgccgcga acgagcggct
      361 ccatcccgtg caggcagcgt tcatccgcaa cgacggcttt caatgcggct actgcacgtc
      421 gggacagatc tgttccgccg tcgcggtcat ccaggaaggg cacgccggca gcgacgatga
      481 gatccgcgag tggatgagcg gcaacatctg ccgctgcggc gcctacgagg gaatcgttgc
      541 cgccgttcgc gaagcatcag ggaggcgcgc atgaatccat tcgatttcgt tcgtgcgcgc
      601 gaagtctccg ccgcactctc ctccggctcg cgcgccgatg cgaagttcat cgcaggcggc
      661 accaacctcg tcgacctgat gaaatgcgac gtcgagaggc cggcgcatct tgtcgatgtc
      721 aatgacctca agctggccgg catcgagcgc gtcagcggcg ggatccgcat cggcgccatg
      781 gcacggatga gcgatgtggc ccgccatccg ctcgtgctcc aaagtgctcc ggcggtttcg
      841 caggcgctgc tggccagcgc gtcgccgcag attcgcaatg cggcgtcgat cggcggcaac
      901 ctgatgcagc gcacacgttg cgcctacttt cgcgagctga cgttcgcccc atgcaacaaa
      961 cgaaatccgg gaagcggttg tgccgcgatc gacggcgaga accgcatgca cgccgttctg
     1021 ggcggctcgg aatcgtgcat cgccacgcat ccgtccgatc tcgcggttgc cgtcgccgcg
     1081 ctcgatgccg tcctcacgct tcacggtccg aatggcgatc ggcacgttgc cgccgccgac
     1141 tttcatctgc tgccgggaac gaccccgcaa accgagcacg atctcaagca cggcgagctg
     1201 atcacgtccg tgttcatccc cgatgcgccg cacgcaaaac gttcggcgta tctcaagatc
     1261 cgcgatcggg ccgcgtacga attcgccctc gcttccgccg ccgtcggtct cgagatcagc
     1321 ggcggcacga tccattccgc gcgcatcgcc ctcggcggcg tggccacgaa accgtggcgg
     1381 gcgaagttcg ccgaggcggc gctgatcggg caagcgccat cgaaggatgt gtttcgtgcc
     1441 gccgcggaag cggagatgaa atccgcggtt gctcacggac aaaacgctta caagatcgag
     1501 ctcgcgaaac ggacgctcgt gcgcgccctc ggcgacctcg cgggaggtgc cgcatgattc
     1561 ccaacatcgg caagccgctc gaccgcgtcg atgggcgaca gaaagtcacc ggcggtgcgc
     1621 actatgccac cgagaacaag ctcgatcgcg tcacctacgc ggcgctggtc gacagcacca
     1681 ttccggcagg gacgatcaaa tcgatcgacg acagccgcgc gaagaaagcg ccgggcgttc
     1741 tcgccgtcat ctcgcacctc aatgcgccga agctcaatcc gccaccacca ccgaagcccg
     1801 gcggtggcgg cgagcaaagc agcagcctca ccgaatcgaa gtacctcccg ttctccgaca
     1861 acaccatcca ttacctcggg cagcacatcg ccgtcgtcgt cgccgacacg ctcgagcatg
     1921 cggtgcatgg cgccgagctg gtgcgcgtcg cttacgacgt cgcgccggcg cgcaccgaca
     1981 tcgagaagta ccgcggcgag gccaaggatc ggtcgcagca atcgccgagc gcgttcacga
     2041 aaggggatgc cgacgccgcg cttgccggtg ccgccgtcaa gatcgatcag atctaccgca
     2101 cgccgcacga gcaccacaac ccgatggagg cgcacggtct aatcgcggcg tgggatggcg
     2161 atcatctaac ccttcacgac tcctcgcaga acatcttcgc cgtgcagcgc accttcgcaa
     2221 cgctgttcga catcccgaaa gaaaatgtgc acgtgc
//
LOCUS       DATLVW010000636         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_361611, whole genome shotgun sequence.
ACCESSION   DATLVW010000636 DATLVW010000000
VERSION     DATLVW010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361611"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VNN25_18590"
     CDS             complement(<1..916)
                     /locus_tag="VNN25_18590"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH93596.1"
                     /translation="MNTSIFTRLLPIGLLAFLSVAALPATAQQQPQTQPETAQTKKDK
                     DKDKETVKPASEEIVVTARKREENVEKVPIAVSVVTADKLEEAATADISELQTQVPDL
                     AVYQGRNQSTTLTVFMRGIGQADPLWGVDPGVGLYLDDVYIARPQGALLDVYDVERIE
                     VLRGPQGTLYGKNTIGGAIKYVSRALTDKTTGSISISPGTQSNRDVRLSFAGALVPGK
                     VRGKIAFASLQHGGYGTNLLTGKDVSNRNTLAGHGALEWLPADKVSVLFGADYTKDEA
                     QPKGYQRLAPNAFCGAFKITCPTLSSHFD"
     gene            1244..2851
                     /locus_tag="VNN25_18595"
     CDS             1244..2851
                     /locus_tag="VNN25_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH93597.1"
                     /translation="MKKLLALIALLATSLFAADTPDLTMQNRIRQEGFRNSKVMDTAE
                     ELMDFVGPRLTGSPNMKHANEWTRDKLTEFGLSNAHLESWGPFGRGWSYEECTVRMTS
                     PDVAQLWALPRAWTPGTNGVITGKPVKVKIETLEDIEKYRGKLAGKIVLNGDVRELKP
                     HEKLQYSRYNEKTLSDESSYELPGRPRFTREEAAKRNAFRKALTKFLAEEKPLLMIDS
                     DSADFGTFHVQGAGTQKDGEQYPVPSIVMSTEHYNRLARLTDLGKSVTLEVNVKTSYY
                     DQDKNAYNTVAEIPGTDKKGEIVMLGGHLDSWHGGTGATDNGAGTVVAMEAVRILKAI
                     GIAPKRTIRIALWSGEEEGLLGSHAYVAEHFASRPEPSAAERDIPSTLRRNAGPLTIK
                     PEHAKLSAYFNLDNGTGKIRGVYTQENYAVAPIFSSWIEPLKDLGVTAITSRNTGGTD
                     HLSFDGVGLPGFQFIQDEVEYGERTHHTNYDVYDRLQRDDLEQAAVVMATFVWEAANR
                     PEMMPRKPLAKGDLPATPAAPAVAPKH"
BASE COUNT          599 a          959 c          901 g          547 t
ORIGIN      
        1 tgtcgaaatg gctgctgagg gttggacaag tgattttgaa cgcgccgcag aaagcgttcg
       61 gcgccagtct ctgatagccc ttcggctggg cctcgtcctt cgtatagtcc gcgccgaaca
      121 gcacactcac cttgtcggca ggcagccact cgagcgcgcc gtggcccgcc agcgtgttgc
      181 ggttggaaac gtccttgcca gtgagaaggt tcgttccgta tccgccatgc tgcagcgacg
      241 cgaaggcgat cttgccgcgc accttccccg gcaccagtgc gccggcgaag ctgaggcgga
      301 cgtccctgtt ggactgcgtt ccgggtgaga tcgagatcga gcctgtggtc ttgtccgtca
      361 gcgcacggct gacgtatttg atcgcgccgc cgatcgtgtt ctttccgtag agcgttccct
      421 gcggaccgcg gagcacttcg atccgttcga catcgtagac gtcgagcaaa gcgccctgag
      481 gacgggcgat atagacgtcg tcgaggtaga gcccgacacc gggatcgacg ccccagagcg
      541 gatccgcctg gccgatgccg cgcatgaata ccgtgagggt cgtcgattga ttgcgtcctt
      601 gataaaccgc gagatccgga acctgcgtct gcagttccga gatgtccgct gtcgccgcct
      661 cctcgagttt gtcggcagtc acgacactga ccgcgatcgg aaccttttcc acgttctctt
      721 cccgcttgcg agcggtgacg acgatctcct cgctggcagg cttcacggtc tccttgtcct
      781 tgtctttgtc cttcttagtt tgtgcggttt cgggctgggt ctggggctgc tgctgcgccg
      841 tcgctggaag cgctgcaacg ctgaggaaag caagcagacc gatcgggaga aggcgtgtaa
      901 aaatggaagt gttcatcaag gccatccttt cgcggagttc atgagatcgc tcggtattcg
      961 gcctgttgtt gcccgcctgg ggttatggaa gcagggagca actcaaactt gccgaccggt
     1021 cggtttgttt gaatccatct tgaaccggcc tcgtctgggt gtcaagcgtt gacgcatcga
     1081 cctccactgc cgacgtctgc ttccggtggt ccctccttcc ggggctgccc aatcgcgaca
     1141 tgcagaggca ggaacaggta agggctgctc tgtggggctc tacggtggct tgaaacaacc
     1201 accaaggcga tgtgccagcc cgattccgct aaactgcgcg gccatgaaaa aactgcttgc
     1261 gctgatcgct cttctcgcga cctctttatt cgctgccgac acgcccgacc tgacgatgca
     1321 gaaccgcatc cggcaggaag ggttccgcaa ttcgaaagtc atggataccg ccgaagagtt
     1381 gatggacttc gtcggaccgc gcctcaccgg ctcgcccaac atgaagcacg ccaacgaatg
     1441 gacgcgcgac aagctcaccg agttcggact ctccaacgcg cacctcgaga gctggggtcc
     1501 tttcggacgc ggctggtcgt acgaggagtg cacggtgcgc atgacgtccc ccgacgtcgc
     1561 ccagctttgg gcattgccgc gcgcctggac gcccggcacg aacggcgtca tcaccggcaa
     1621 acctgtcaag gtgaagatcg agaccctcga agacatcgag aaatatcgtg gcaagcttgc
     1681 gggcaagatc gtcctcaacg gcgatgtccg cgagctcaag ccccacgaga agcttcagta
     1741 ttcgcgttac aacgagaaaa cactgagcga cgagagcagc tacgaattgc cgggccgccc
     1801 ccgtttcaca cgcgaagagg cagccaaacg caacgcgttc cgcaaagcgc tgacgaagtt
     1861 cctagcggaa gaaaagccgc tcctgatgat cgactcggat tcagcggact tcggcacgtt
     1921 ccacgtccag ggcgccggca cgcagaaaga cggtgagcag tatccagtcc cgtcgatcgt
     1981 gatgtcgacc gagcactaca accgtctggc acgcctcacc gatctcggca agagcgtgac
     2041 tctcgaggtc aacgtcaaga cctcttacta cgatcaggac aagaacgcct acaacacggt
     2101 cgccgagatc cccggaaccg acaagaaagg cgagatcgtc atgctgggcg gccacctcga
     2161 ctcatggcac ggcggcacgg gagctaccga caacggcgcc ggcacggtgg tggcgatgga
     2221 ggccgtgcgc attttgaagg cgatcggcat cgccccgaag cggacgatcc gcatcgctct
     2281 ttggagcggc gaggaggaag ggcttctcgg ctcgcacgcc tacgtcgccg agcatttcgc
     2341 ttcccgtccg gagccgtccg cggccgagcg cgatatcccc tcgacgttgc gccgcaacgc
     2401 cggtcctctg acgatcaaac cggagcacgc aaaactctcg gcctacttca acctggataa
     2461 cgggaccggc aagatccgcg gcgtctacac gcaggaaaac tacgccgtcg cgccaatctt
     2521 ttcatcctgg atcgagcctc tcaaagacct cggcgtcaca gcgattacct cgcgcaacac
     2581 cggcggcacc gatcacctct ccttcgatgg cgtcggtctt ccaggcttcc agttcattca
     2641 ggacgaggtc gagtacggcg agcgaacgca ccacacgaac tacgacgttt acgaccgcct
     2701 gcagcgcgat gatctcgagc aggccgccgt ggtgatggcg acattcgtgt gggaggcggc
     2761 gaaccggccg gagatgatgc cccgcaaacc gctcgcgaaa ggcgatctgc cggcaacgcc
     2821 agcagcgccg gcagttgcac cgaagcactg atcgcggtca gaaacctcaa ggttgcgaag
     2881 gtcccgcggt gcccaggtgt tacgggcacc gcgggatcgc gcacccagcg acgcgaaaca
     2941 gtcgcaaccg tctcatcctc cgacacccgg cccccgacga gcgactcccg cccaggcgat
     3001 cacgac
//
LOCUS       DATLVW010000637         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_438080, whole genome shotgun sequence.
ACCESSION   DATLVW010000637 DATLVW010000000
VERSION     DATLVW010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438080"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1525)
                     /locus_tag="VNN25_18600"
     CDS             complement(11..1525)
                     /locus_tag="VNN25_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93598.1"
                     /translation="MPKKRQRRNPVPKAPPKRRFAGQPVPERQLTSALQRVRAMSGQL
                     DERERAFDPSRAPTWRGSSRDLVVNGSMDEFTRGFLETALWSSNDESDDSGGEPLDAN
                     YSIEDFDPKSLEGLERDCERFQSENADDLAVCYETGIRGQSDEFSAGHDFWLTRCGHG
                     AGFWDGDYPEPQATRLSEASENFGNVDLYVGDDGQIYASGYESGTDKNYRRNAEQLSF
                     KGMGVGPHAAGGIELREFDWEQVDGDVNLCAHGGIIARSNGKEIDLIEIQPVREYVGD
                     GEAAEVGFPFWTKEAYYDLSDLSPSNKNVKAAMESSDLDLSEIDPEHRGVAIALACMR
                     YGHGSEEGDGGWSKDIVQFPVRWGYAGTERMTFEEYCGDEDEEFRVEVLGEGYTYKIA
                     YEIITPESAEDGEAEERGWEEEGSDIASLADTIRAASDHNWIEWSDSNIGPGSWLISE
                     EEQDMHTGASTVYSLWIERSDKEPLNKDEILEINKAFGISNFGRMLPNRRKRSR"
     gene            complement(1532..2230)
                     /locus_tag="VNN25_18605"
     CDS             complement(1532..2230)
                     /locus_tag="VNN25_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93599.1"
                     /translation="MSEAFRSAVAFRNDICDVISQLEQRFTRPLPNGATVYVTGGDAL
                     YRLVKDLGSSFDTLGSAIVVPADGTDNRWVAESISGASPWSSIEVALANVDVVPNNPD
                     AWRGLGTTAGSFGGGSTIFEVNPTTSVMTYRGVTRQMTLAYEATILNGTGGPIDAEAA
                     ISHNGDVPEDSTTDFRGDGQQGTTLAAGFRAVVSGKRSVLLAPNQTVRLMLRNVAGSG
                     TLTVQYFSMSFAPN"
     gene            complement(2340..>2554)
                     /locus_tag="VNN25_18610"
     CDS             complement(2340..>2554)
                     /locus_tag="VNN25_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93600.1"
                     /translation="IGVASTEIGRPLPLGAKIVGYGPLAQGSVVPLDRGGLSGIPDAA
                     GSPLGIAVIGALSAVAGVLLVRVIRK"
BASE COUNT          441 a          844 c          752 g          517 t
ORIGIN      
        1 gtggccatgt tcagcgactc ctcttgcgac gattgggcag catacgaccg aagttgctga
       61 tcccgaaagc cttgttgatc tccaggatct cgtccttgtt cagcggctcc ttgtcggaac
      121 gctcgatcca gagactgtag accgtgctcg ccccggtgtg catatcttgc tcctcttcgc
      181 tgatgagcca cgagccgggt ccgatatttg aatcggacca ttcgatccaa ttgtgatcgc
      241 tggcagcacg aattgtatcc gcgagcgaag cgatgtctga gccttcctct tcccagcctc
      301 gttcctctgc ctcgccatcc tctgcgctct caggcgtaat gatctcgtag gcgatcttgt
      361 aggtgtagcc ttcgcccaac acctcgaccc ggaattcctc atcttcgtcg ccgcagtact
      421 cttcgaaggt catgcgctcg gttccggcat acccccagcg taccggaaat tgcacgatgt
      481 ccttggacca gccaccgtca ccttcttccg agccatgtcc gtaacgcatg caagccaggg
      541 cgatagctac gccacggtgc tcgggatcga tctcgctgag gtccagatca ctggattcca
      601 tagcagcctt gacattcttg ttcgaggggg acagatcgga caggtcgtag taggcctctt
      661 tcgtccaaaa cggaaacccg acttcagccg cttcgccgtc acccacgtac tcgcgcaccg
      721 gctggatctc gatgagatcg atctctttac cgtttgagcg cgcaatgatg ccgccgtgag
      781 cgcagaggtt tacgtcgcca tcgacctgct cccagtcgaa ctcgcgtagc tcgatgccgc
      841 cagcagcgtg cggcccaacg cccatcccct tgaacgacaa ctgctcggcg ttgcgccggt
      901 agttcttgtc tgtgccgctc tcgtaaccgg aggcatagat ctggccgtcg tcgcctacgt
      961 agagatcgac gttcccgaaa ttctcactgg cttcgctcag gcgcgttgcc tgcggctccg
     1021 ggtagtctcc gtcccagaat cctgcgccat ggccgcagcg cgtgagccag aagtcgtgcc
     1081 cggcactaaa ctcgtctgac tggcctcgga tgcccgtttc gtaacagaca gccaggtcgt
     1141 ctgcgttttc cgactgaaag cgctcgcagt cgcgctcgag tccctcgaga gacttcggat
     1201 cgaagtcttc gatcgagtag tttgcgtcga gcggttcgcc accactgtcg tccgactcgt
     1261 cgttcgaaga ccaaagcgcc gtctccagaa acccccgcgt gaactcgtcc atgctcccgt
     1321 tcacgaccag atcccgcgac gacccccgcc acgtcggcgc ccggctcgga tcgaacgccc
     1381 tctctctttc atccagctgc ccgctcatgg cgcgcacgcg ctgaagcgcc gaggtgagct
     1441 ggcgctcggg cacgggctgc cccgcaaagc ggcgcttggg cggcgccttg gggacggggt
     1501 tccgacgctg gcgtttcttg ggcatggttc ctcagttagg cgcgaacgac atcgagaagt
     1561 actgcaccgt gagcgtgccg ctgccagcca cgttgcgcag catgaggcgc accgtctgat
     1621 tgggcgcgag cagcaccgag cgtttgccgg aaacgacggc acggaagcca gcagcaagcg
     1681 ttgtgccttg ctgaccatca cctcggaagt cggtcgtcga atcttcgggt acgtcgccgt
     1741 tgtgactgat cgcagcttcg gcgtcgatgg gaccaccggt gccgttcagg atggtcgctt
     1801 cgtaggcgag ggtcatctgc ctggtgacgc cgcggtacgt cataacgctc gtcgtcgggt
     1861 tcacttcgaa gatcgtcgat ccacccccga agctgcccgc tgtggtcccg agtcctcgcc
     1921 aagcatcggg gttgttgggc actacatcga cgttcgcgag cgccacctcg atgctcgacc
     1981 aagggctcgc gccgctgatg ctctcggcca cccaccggtt gtccgtgccg tccgccggca
     2041 ccacgatggc agagcccagc gtgtcgaatg agctaccgag atccttgacg agccggtaga
     2101 gcgcatcgcc gcccgtcacg tagacggtag cgccgttcgg tagcggccgc gtgaagcgct
     2161 gctccagctg gctgatgacg tcacagatat cgttgcggaa agcgactgca gatcggaatg
     2221 cctctgacat ggggtcagcc taccggaggc aggagctttc ctccaaaaag tgggcagact
     2281 ccatcgctac cctgggtttt ctacgcggtt tccgggaccg cggatcgacc cgggcagttt
     2341 tacttgcgga tgacgcgcac gagcagcacc ccagccaccg cgctcagcgc gccaatgaca
     2401 gcgataccaa gcggcgatcc ggcagcgtcc gggatccctg agaggccgcc gcgatcgagc
     2461 ggcacgaccg agccctgcgc gagaggtccg tagcccacga tcttggcgcc gagcgggagc
     2521 gggcgcccga tctcggtgct cgctactccg atgg
//
LOCUS       DATLVW010000638         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_397457, whole genome shotgun sequence.
ACCESSION   DATLVW010000638 DATLVW010000000
VERSION     DATLVW010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397457"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="VNN25_18615"
     CDS             complement(<1..1549)
                     /locus_tag="VNN25_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXH93601.1"
                     /translation="MDDEHGEHGGHHQSWIRRYVFSTDHKWIGIQYGFTALCFLLLGF
                     CLMILMRLQLGWPGQPFAIMKLLGATRAPGGILLPEAYNQLGAMHGTIMIFLGIVPLA
                     VGAFGNYLVPLQIGAPDMAFPKLNMASYWCYLPGGLIMLASFLAPGGTAQSGWTSYPP
                     LSVIASQGQTWWLIGMVFLITSSLLGSVNFIVTIIQLRAKGLSYMRFPFFVHAQFVTS
                     FLLLLAFPPLEAAAVLQLMDRLAGTSFFLPSGLVVSGQALTNAGGGNPLLWQHLFWFL
                     AHPEVYVLILPAMGIAAEIIANGTRKPLWGYKSMVFATVFLGFMSFVVWAHHMFLTGM
                     GTTISAFFQTTTMIISIPSVIILSSLFISLWGGSIRFTTPMLFALAFLPMFGIGGLTG
                     LPLGLAAADIHLHDTYYVIGHFHYVVAPGTIFAMFAGIYYWFPKATGRKLNEFWGRVH
                     FWISFVCINGIFFPMFAQGLAGVSRRLYDGGMQYQHAKQIAAQTNPFMSICAIGLLAA
                     QVPFIFNF"
     gene            complement(1675..2493)
                     /locus_tag="VNN25_18620"
     CDS             complement(1675..2493)
                     /locus_tag="VNN25_18620"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH93602.1"
                     /translation="MKKFVSLLFATTLVFSIACGKGKEASKEDQGEDDETQTATAATN
                     TASTAAAAAPAAAVSADAATLTGTVKFEGAAPKMASIQMSADPYCQSQHGSAPAMEED
                     SVVGPAGELANVFVYIKDIKGNFPAPTTAVTIDQKGCQYHPHVNAVMVGQPLDIKNDD
                     NTLHNIHALPVVNSQFNEGQPVQGMVSTKKLDKAEMTPFKIKCDVHGWMKSYMAVMPH
                     PFYAVSQTNGTFTIPNLPPGSYTVVAWHEKYGQQEQQITVGAKESKAMSFTFKG"
     gene            complement(2508..>3096)
                     /locus_tag="VNN25_18625"
     CDS             complement(2508..>3096)
                     /locus_tag="VNN25_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93603.1"
                     /translation="SRGMNPPAQNVAIGKQVFLMMRCTQCHGTTPVNPENPPVPNTAD
                     STSLAPNLTLARMRLRHEWISDWIRRPNEMINGTRMPTNFPRDAATGGFQSPLAMAID
                     TPPFAQYKATLLPLFNNDANELKRTMGDAVALTDYLRDYIWSIGITQMRTAAPGETVP
                     AIAMPQPSLPPPTAPALKSTRNQRGVAGNSGGPGR"
BASE COUNT          623 a          909 c          972 g          592 t
ORIGIN      
        1 agaagttgaa aatgaacggc acctgcgcgg cgagcagccc gatcgcacag atcgacatga
       61 acgggttcgt ttgtgccgca atctgtttgg cgtgctgata ctgcattccg ccgtcgtaaa
      121 ggcggcgcga gacaccggca agcccctgcg cgaacatcgg aaagaagatg ccgttgatgc
      181 agacgaatga gatccagaag tggacccggc cccagaactc gttgagctta cggcccgtcg
      241 ccttcgggaa ccagtagtag atgccggcga acatcgcgaa gatcgttccc ggcgcaacga
      301 cgtaatggaa gtgcccgatg acgtagtagg tatcgtgcag gtggatgtcg gccgcagcga
      361 gaccgagcgg caatcccgtc aaaccgccga tgccgaacat gggaaggaac gccagcgcga
      421 acagcatcgg ggtcgtaaat cgtatcgatc cgccccataa cgagatgaat aacgagctga
      481 gaatgatgac cgacgggatg gagatgatca tcgtcgtggt ctggaagaat gcgctgatcg
      541 tcgtgcccat gcccgtcagg aacatgtggt gtgcccaaac gacgaacgac atgaacccga
      601 ggaagacggt ggcgaacacc atcgacttgt agccccagag cggcttgcgc gtaccgttcg
      661 cgatgatctc ggcggcgatt cccatcgccg gcagaatcag aacgtagacc tcaggatggg
      721 caaggaacca gaagagatgc tgccagagca gcggattacc acctccggca ttcgtcagag
      781 cctgaccgct gaccacgagg ccgctcggga ggaagaagct cgtcccggcg aggcggtcca
      841 tgagctgcaa cacggctgcg gcttcgagcg gtgggaaggc gagcaacaaa agaaaggacg
      901 tcacgaactg cgcgtgcacg aagaagggga agcgcatata cgacagcccc ttggcgcgca
      961 attgaatgat agtcacaatg aaattcacgg agcccagcaa cgacgaagtg atcaggaaaa
     1021 ccatcccgat cagccaccac gtctgccctt gcgatgcaat gacgctcaat ggcgggtacg
     1081 acgtccagcc cgactgcgcg gtacctcctg gcgcgagaaa actcgccagc atgatcagac
     1141 cgcccggcag gtaacaccag tagctggcca tgttgagctt cgggaaagcc atgtcgggcg
     1201 cgccgatctg caacggcacg aggtaattgc cgaacgcacc cactgccagc ggcacgatgc
     1261 cgaggaagat catgatcgtg ccgtgcatcg ccccgagctg gttataagcc tcgggtagca
     1321 gaatcccgcc aggcgcgcgc gtcgcgccga gcagcttcat gatggcgaac ggttgtcccg
     1381 gccagccgag ctgcaggcgc atcaggatca tcagacagaa gccaagcagc aggaagcaga
     1441 gggcagtgaa gccatactgg atgccgatcc acttatggtc cgtggagaag acatagcgcc
     1501 gaatccacga ctgatgatgt ccgccgtgct cgccatgctc gtcgtccatg acacgaagcg
     1561 cctcgcggag cgccctgtgc gcttcatccg cgtacatcca ttgcgaacct acccggatct
     1621 gtcgtctcag tcccatgttt tagctcttta gatgctggct cgtagttacg cgcgttatcc
     1681 cttgaacgtg aaactcatcg ccttcgactc tttcgcgccg acggtgattt gctgctcctg
     1741 ctgaccatac ttctcgtgcc acgccaccac cgtgtaggaa cccggcggga gattggggat
     1801 cgtgaacgtg ccgttggtct gtgacaccgc atagaacgga tgcggcatga cggccatgta
     1861 cgacttcatc cagccgtgaa cgtcgcactt gatcttgaac ggcgtcattt cggccttgtc
     1921 gagtttcttc gtagagacca ttccctgcac cggctggcct tcattgaact gagagttgac
     1981 gactggcaag gcgtggatgt tgtgcagcgt gttgtcgtcg ttcttgatgt ccagcggctg
     2041 tccaaccatg acggcgttga cgtgcgggtg gtactggcag cccttctggt cgatggtcac
     2101 cgctgtcgtc ggcgcgggga aattgccttt gatgtccttg atgtagacga agacattggc
     2161 gagctcgccg gcggggccga caaccgagtc ctcttccatc gctggagcgc tgccgtgctg
     2221 cgactggcag tacggatcgg ccgacatctg aatgctcgcc atcttcggcg cggcgccctc
     2281 gaacttcacc gttccggtga gcgtcgctgc gtcggcagaa accgcggcgg ccggcgctgc
     2341 ggctgctgcg gtcgaggccg tgttcgtggc agcggtcgcc gtctgcgtct catcatcctc
     2401 gccctgatct tctttcgatg cttctttgcc cttgccgcag gcaatcgaga aaacgagtgt
     2461 cgttgcgaac agcagtgata cgaacttctt catggtctgt ctcctgtcta acgtccgggg
     2521 ccgccgctgt tgccggcgac gccacgctga ttgcgagttg acttcaatgc cggcgccgtt
     2581 ggcggcggca gcgacggctg cggcatggcg atcgccggaa cagtctcgcc cggggccgca
     2641 gtccgcatct gggtgatgcc gatgctccag atgtaatcgc gcagatagtc ggtcagagcg
     2701 accgcgtcac ccatcgtgcg cttcaattcg ttcgcgtcgt tgttgaagag tggcagcagc
     2761 gtcgctttgt attgggcaaa cggcggcgtg tcgatggcca tcgccagcgg cgactggaaa
     2821 cctccggtcg cggcgtcgcg cgggaagttc gtcggcatgc gtgtgccgtt gatcatctcg
     2881 ttcgggcggc ggatccaatc ggagatccat tcgtgtcgca ggcgcatccg cgcgagcgtg
     2941 aggttcggcg cgagcgaggt cgagtcagcc gtgttcggca ccggcggatt ctcggggttg
     3001 acgggggtcg ttccgtggca ctgcgtgcag cgcatcatca aaaagacctg tttcccgatc
     3061 gcgacgttct gcgcgggcgg attcatcccg cgcgac
//
LOCUS       DATLVW010000639         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_480770, whole genome shotgun sequence.
ACCESSION   DATLVW010000639 DATLVW010000000
VERSION     DATLVW010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480770"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="VNN25_18630"
     CDS             complement(<1..1667)
                     /locus_tag="VNN25_18630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH93604.1"
                     /translation="MSDAPTFASLGLSTPLLETLTQLGYEAPTPIQARTIPALLKGRD
                     LIGQAQTGTGKTAAFALPILQQLDLSSRVTQALVLAPTRELAVQVSEAMHTYAKKLEG
                     VTVLPVYGGAPIFGQMKHLQRGAHVVVGTPGRLIDHLDRGTLSLEHVRMIVLDEADEM
                     LKMGFIDEVERMLSLVPKPRQVALFSATMPEPIARIAQKHLVNPERVEIEHHTIAGPD
                     IEQRFLNLAEGQKLEVLTQMLELEEADAVLIFRRTKNGAAELAEKLGGRGFVAEAMHG
                     DMKQAERESVIRRLRAGKLDIVVATDVAARGLDVEQITHVVNYDIPNDVEAYVHRIGR
                     TGRAGRSGVATLFITPRERRMMREIERFTGTQIKPMKMPSRADVAARRIAVFKESLRK
                     ELQAGDLDLYVEVVTQLAEEGPFDMADVAAAASRIAHGTRAVAAPAEAVKREAPATAS
                     SPRDDDEDAKVRLSMALGKRDGIRPADVVGSIANEAGIPGSDIGPIEIRDEITYVTIP
                     ARHREIVLAKLGSAHFRGKPVNLRVAGGESPSRERPRYDRERPTPRPA"
     gene            1799..2080
                     /locus_tag="VNN25_18635"
     CDS             1799..2080
                     /locus_tag="VNN25_18635"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HXH93605.1"
                     /translation="MRDTQNKTTISVRLSADEERSVRLLAKRKRRTVSDVIRDAVAGI
                     VEDDRKTISRPYDDISDLIGSVTGLPPNLSEGSGDQFAAILREKAGRRK"
     gene            2077..2499
                     /locus_tag="VNN25_18640"
     CDS             2077..2499
                     /locus_tag="VNN25_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH93606.1"
                     /translation="MILIDAGPMVALISASDNHHEICRTTLESITEPLVTVWPAFTEA
                     MYLLRWSWRAQRALWEMIERGTIDLHYLNINDARRMRELMEKYRDLPMDLADAALVRT
                     AEREKIKRIFTLDRRDFSVYRPLGVGRFTILPAKLVRR"
     gene            complement(2596..>3065)
                     /locus_tag="VNN25_18645"
     CDS             complement(2596..>3065)
                     /locus_tag="VNN25_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXH93607.1"
                     /translation="EFLTETRGTGLLYHTFLEYGPYRGEIAGRNRGALISKEAGDTTA
                     YAIEPLQERTVLFIAPGLKVYGGQIVGENSRDADMVVQVCRKKHLTNMRASGSDMAVQ
                     LTPPKIMTLEQCLEWIEDDELVEVTPQSIRIRKRILDHSMRVVAQKKIAAGSS"
BASE COUNT          551 a          955 c          966 g          593 t
ORIGIN      
        1 gcgggacggg gagtgggacg ttcgcggtca tagcgaggac gttcgcgtga aggggattcg
       61 ccgccagcga cccgcagatt caccggcttc ccgcgaaaat gtgcgctgcc gagtttcgcc
      121 agcacgatct cgcgatgacg agcggggatc gtgacgtatg tgatctcgtc gcggatctcg
      181 atcgggccga tgtcgctgcc gggaatgccc gcctcgttcg cgatcgatcc cacgacatcc
      241 gcgggacgga taccatcgcg cttgcccaat gccatcgaca ggcggacctt cgcatcttcg
      301 tcatcgtcgc gcggtgaaga ggccgtggcc ggcgcttccc gcttcaccgc ctcggctggc
      361 gcagcaacag cccgcgttcc gtgtgcgatc cgcgatgccg cggcggcgac atcggccata
      421 tcgaacggcc cttcctcggc gagctgcgtg accacctcga cgtagagatc gagatcgccc
      481 gcctgcaact ctttgcgcag cgactctttg aaaacagcaa tgcgccgcgc ggcgacgtct
      541 gcgcgcgatg gcatcttcat aggcttgatt tgcgtgccgg tgaatcgctc gatctcgcgc
      601 atcatccgcc gttccctcgg cgtgatgaac aacgtcgcga cgccgctgcg gccggcccgt
      661 ccggtgcgcc cgatgcgatg cacgtacgcc tcgacgtcgt tcggaatgtc gtagttgacg
      721 acgtgcgtga tctgctcgac gtcgagcccg cgcgcggcga cgtcggtagc aacgacgatg
      781 tcgagcttgc cggcgcgcaa tctgcggatg acgctctcgc gctcggcttg cttcatatcg
      841 ccatgcatcg cctcggcgac gaatccgcgt ccgccgagtt tttccgccag ctccgccgcg
      901 ccgtttttcg tgcgccggaa gatgaggacc gcgtccgctt cctcgagctc gagcatctgc
      961 gtcagcactt cgagcttctg tccctcggcc agattgagaa agcgctgctc gatgtcgggt
     1021 ccggcaatgg tgtgatgttc gatctcgacg cgctcaggat tgacgagatg cttctgggcg
     1081 atgcgcgcga tcggctccgg catcgtcgcc gaaaagagtg ccacctgcct cggctttgga
     1141 acgagcgaaa gcatccgttc cacttcatcg atgaagccca tcttcagcat ctcgtccgct
     1201 tcatcgagca cgatcatgcg cacgtgttcg aggctgagcg tgccgcggtc gagatgatcg
     1261 atgagccgtc cgggcgtgcc gacgacgacg tgcgcgccgc gctgcagatg cttcatctgc
     1321 ccgaaaatcg gcgcaccgcc gtaaacggga aggacggtaa cgccctccag tttcttcgcg
     1381 tacgtatgca tcgcctcgga gacctgcaca gccagctcgc gcgtaggcgc aagcaccagc
     1441 gcctgggtga cgcggctgct cagatcgagt tgctggagaa tcggaagcgc gaacgcggcc
     1501 gtctttcctg tgcccgtctg ggcctgcccg atgagatcgc gtcccttcag cagcgccggg
     1561 atcgtccgcg cctgaatcgg cgtcggcgcc tcatatccaa gctgcgtcag tgtttcgagg
     1621 agtggagtgg aaagaccaag agaagcgaag gtgggagcgt ccgacatagg ggtcccactg
     1681 tatcaattcg actgaagaaa cgcagggacc atccgcagat gacgcagatg gaaggcgggt
     1741 tcatcgatga attctccgtc acttgcggat ggcgctagtt ttgtattaca cttctgtcat
     1801 gcgcgacaca cagaacaaaa cgacgatcag cgtgaggttg agcgcggatg aagaacgcag
     1861 cgtgcgcctt ctggcgaagc ggaagagaag gactgtctcc gacgtcatcc gcgacgcggt
     1921 ggctggcatc gtcgaagatg accggaagac gatcagccgc ccgtacgatg acatctccga
     1981 tctcatcggc tccgtgaccg gtcttccgcc caacctgtcg gaagggagcg gtgatcagtt
     2041 cgcggcgatc ctgcgagaga aagccggacg ccggaaatga tcctcatcga cgccggcccg
     2101 atggttgccc tcatcagcgc cagcgacaat catcacgaga tctgccgcac aactctcgaa
     2161 agcatcaccg aaccgctcgt caccgtgtgg cccgcattca ccgaggccat gtatttgctg
     2221 cggtggtcat ggcgtgcgca acgcgcgcta tgggagatga tcgagcgcgg gacgatcgac
     2281 ctgcactacc tgaacatcaa cgacgcccgc cgcatgcgcg agctgatgga gaagtaccgc
     2341 gacctcccga tggacctcgc cgacgccgcc ctggtccgaa ccgccgagcg cgagaaaatc
     2401 aaacgcatct tcactctcga ccgccgcgat ttcagcgtct accggccgct gggtgtggga
     2461 cggttcacca tcctgccagc gaagttagtc cgcagatgac gcagatagaa gacgcagatt
     2521 gagtaccgtt caatctgtga atatctgcgt catctgcgga ttgggtctca ccggcagaat
     2581 cgactgtgtg gccgcctacg agctccccgc cgcgatcttc ttctgcgcca ctactcgcat
     2641 cgagtgatcg aggatgcgtt tgcggatgcg gattgattgc ggggttactt cgaccagctc
     2701 gtcgtcttca atccattcga ggcattgctc gagcgtcatg atcttcggcg gcgtgagctg
     2761 gacggccatg tcggagccgc tggcgcgcat gttcgtgagg tgcttcttgc ggcagacctg
     2821 gacgaccatg tcggcgtcgc gggagttttc gccgacgatc tggccgccgt agactttgag
     2881 gccgggcgcg atgaagagaa ctgtgcgctc ctggagtggc tcgatcgcgt aggcggtcgt
     2941 gtcgccggct tccttgctga tcaacgcccc gcggttgcgg ccggcgatct cgccacggta
     3001 cgggccgtac tcgaggaacg tgtggtacag caagccggtg ccgcgggttt cggtgaggaa
     3061 ctcgg
//
LOCUS       DATLVW010000640        13138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_480798, whole genome shotgun sequence.
ACCESSION   DATLVW010000640 DATLVW010000000
VERSION     DATLVW010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480798"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VNN25_18650"
     CDS             <1..220
                     /locus_tag="VNN25_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93608.1"
                     /translation="AGDDTWRAYQQSDIRKNEGARRDNPPKTITYVAGPATSKKQFLL
                     QTETGVIDAMSAPEEQAIAALAQALGLK"
     gene            220..2571
                     /locus_tag="VNN25_18655"
     CDS             220..2571
                     /locus_tag="VNN25_18655"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93609.1"
                     /translation="MSTNPFPGLRPFQEEEEHLFFGRESQVDAMVDTLAASHFLVVVG
                     TSGSGKSSLVNCGLRPALHRGMMARAGTSWRIAQFRPGNNPICAMAEALAKEGALYSD
                     AAEGPFSQSEIIESSLRMSKLGLLDVFQQARLPQGTNLLLVADQFEELFRFQEQSAIG
                     TNARNDLADQAAAFVNLLLAVREHKDQPIYVTLTMRSDFLGECAQFYGLPEAINGGQY
                     LVPRMTRDERRRAIAGPVGVGGAEIDPVLLTRLVNDVGANPDQLSILQHALNRTWRQW
                     EREGSKGPLTLQHYEKIGTMAGALDQHAEKAYAELSTPREREICEKLFKAITDKASDA
                     RGVRRPTKVDTLCKLTGATIEELTAVVDVFRKPSRSFLMPPFGEALKADTVIDISHES
                     LMRVWDRLRVWTEEEARSAGTYHRLSETASLEAEGKASLWTGPDLAGALAWLNDEKPE
                     ACWAERYAPGFNEAMAFLKKSADAQAAEQKRQLDEVLEKARTQARYARAIALLSVALI
                     LALVLIGIIYHRSVTTAHEAENKKHKESEESLQKQVVTSQVVALQAFAIDKQKPEDAR
                     KVRQSFEALAHISAITATTSPEQHSNTRIQYFKKATDSPKLVNALKDLGFEDVSERTA
                     SNPRETNGIWYGARVPENDVKLVAFALMRAGIDLQSIQPSLRIGPEVIQVGHNPWVQD
                     QPPFTPDVVSKTPLGQLRRPEARTKADVIGTITKLDPKAREGWIKGPEGPVYFQFSPD
                     PPPKPFNVGDAVTFTVYYGKRMYAEGVTARPATPGPQSLRRGS"
     gene            complement(3075..3482)
                     /locus_tag="VNN25_18660"
     CDS             complement(3075..3482)
                     /locus_tag="VNN25_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HXH93610.1"
                     /translation="MTEIAERWAEPAIRVLMMPRDTNAQGTIFGGVILSYIDQAGAIE
                     ARRQGSRLMLTVSMDKIVFHQPVFVGDLISFWTETLRIGRTSITVKVVCEAIRGTDPQ
                     KKVLVTEAQVVYVNVGEDKKPVPIERVANALSR"
     gene            complement(3605..5554)
                     /locus_tag="VNN25_18665"
     CDS             complement(3605..5554)
                     /locus_tag="VNN25_18665"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXH93611.1"
                     /translation="MLKLMRDSFQHLKWILVAIVAVFILFIFVDWGAGGAGNGGGIDQ
                     RAYAARVNGETISYREFDRALYYTEKNYEQVYHQPLTPDQVQQLGLQKQVLDSLIDQH
                     LLLQQARRLHLSASAEEVRKKILDLPTLNPDGKFVGAEIYTRYVTMMGYQSPAEFEEE
                     LAREITLAKMESAIQNSIIISPKSADDEYRRTSETARIKYVLYPAAKEAANVTVTPAE
                     VQQYYTTHQSQYTHGEQRAAKYLIADYAKVRASINPSDAEVHKIYDQRHEEFKAPEGA
                     HILHILIKVDQGAPAPVDQAAKAKADDIVRQLRAGADFGKLAKENSGDPSSAGKGGDM
                     GWVDKGTTVEPFDKAAFTMPLNTISDPIHSVEFGYHIIKVLERRPPGYHTFEEVRDRL
                     RGLTIDQQAKDRARDEITSIAARLKESKPKSAPEFTALASGDVASNDTQWFGKADPIP
                     GMGNNPAFASWAFAAKQNDVGDIIGTQRGPAIPFLYNIRSAGVSPLDEVRAKVENDAK
                     MEKARMLGQQALAKELPAPTVDLAATKLGLASAETSVNRQGFVTGFTGDTSALVNAAM
                     TAPVGQVKGPVMTGDGAVIFQVLEQKKVDPATLAQNRGQYMENLRQQESRNLRATMLQ
                     VLRKAASIDINKKVTEQQTPSQQSS"
     gene            5718..7154
                     /locus_tag="VNN25_18670"
     CDS             5718..7154
                     /locus_tag="VNN25_18670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXH93612.1"
                     /translation="MAKKKKQSSEPAIDWYLVSIERLKQIGIVILVVLLGGGAWWYYT
                     NQKRNPRSSAEAAIAEARQALNTLAASKDFPMHRSDFDRAQHKLDEAGTKLAAGNYPD
                     AESAAVESQTFSRAALSGKGGADNDAQFLTVEGDVQFQKSSTSDWSAAETRTPLFNGD
                     WVKTGRSASAELIFSNGSLYTIGPNALLEIYSQFNAATSRKNNSVQMQVGSVEVATTD
                     DASSVRTPGSQVTIDSESTTQVGVDEQKTTAVLSEKGSASIAPSDGSAAVKLAVGDKV
                     TATPQGALSAVRKLPMPPALLTPSDNQVFLVTADSRVQFSWEAVPGAAGYVLQVSRSR
                     LFTSLEINSTRQKTTASAQVTAEGAFYWRVAATEAGGDTGPFSAFRRFRVNGPGHEGT
                     PALDAPAPRLLLKRPYSIGGPFFMIEGDTDPGATVLINDEDADVEGNGHFKKLVSFNK
                     VGTNLVVIKATNAAGKQTRQSETVHVEE"
     gene            7415..8440
                     /locus_tag="VNN25_18675"
     CDS             7415..8440
                     /locus_tag="VNN25_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXH93613.1"
                     /translation="MALKSLLSMFSNDLAIDLGTANTLVYAKNKGIVVREPSIVAVNK
                     INNNVEAVGKEAKEMLGRTPGNIVAIRPMKDGVIADFEVTEKMIKYFIEKAHGRRFLV
                     KPRIVISVPSEITQVEKRAVKDSALRAGATEVYLIEQAMAAAIGAGLPITDPTGNMIV
                     DIGGGTTDVAVISLAGIVYSRSVRVAGNEMDDAIIQYIKRKYNLLIGERTAEQIKMEI
                     GSAFPLDEEMTMEIKGRDLVEGVPKTLVVSDEEIREALSETVATIVEAVRVALERTPP
                     ELSADIMDKGIIIAGGGSMLKNLDKRLREETGLPVTLAEDPLACVALGTGQMLTDFNL
                     LRKISIE"
     gene            8671..9531
                     /gene="mreC"
                     /locus_tag="VNN25_18680"
     CDS             8671..9531
                     /gene="mreC"
                     /locus_tag="VNN25_18680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXH93614.1"
                     /translation="MAAPADVIPRRPTLLFIVVLALLFFLMSLSSRTRYIGETRTMFE
                     RSVMILFAPIPKTVNWAGQSMSDIYHGYLDMRRSVGENLQLHRQIDELTTENLRLRQS
                     EGDLRRLRSLLGYSEEFTMPTTMAQPVMLDISGRFKSIILDRGSNSGIEVNDAVVNAN
                     GLIGRVVLTTKDMAKVQLITDGNSSVGVLIDRTRRQGVLRGDSAAGTQLYDIPSLADV
                     RLGDVVMTAGIDGIYPKGIPVGVVTKAEKGQDLFKTIVVRPSIDFGSIEEVIVLHTRK
                     IPNDVVRYTP"
     gene            9528..10034
                     /gene="mreD"
                     /locus_tag="VNN25_18685"
     CDS             9528..10034
                     /gene="mreD"
                     /locus_tag="VNN25_18685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXH93615.1"
                     /translation="MRWLRFAVGLLIALTLHSLLDSFFPNALAFFNPYVILVVYYSMG
                     GNVFGAIIAGVIAGLVQDAYSGAMFGLHAFALTVCAYAVAYINSRLVLRGTLAFGACL
                     IGAVIVNELVIYILVNILLKQKVEMFEQGMIVKTVFTCLFGMIVYLLLTLFLREEPID
                     ATRRPTGW"
     gene            10182..12164
                     /gene="mrdA"
                     /locus_tag="VNN25_18690"
     CDS             10182..12164
                     /gene="mrdA"
                     /locus_tag="VNN25_18690"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXH93616.1"
                     /translation="MRVYRDDQKFLTFRINALLWAIVIVFVFLAGSFWVVQGVQAEKY
                     RNLSEANALRELVTPAKRGLILDRSGQKILADNQPAYSLSLDRVVMRPIIKNDPSHRD
                     KLVAFLAQVLGVPTQEIEARFEKGKAIPNARPIPVAEDLSMTQVASIQAQAIAFPELD
                     VEPVQRRNYPYATMAAHVMGFIGEANEKDLAAHKELRSGDLLGKRGVEMMYDEYLRGR
                     DGAQYWQYDSHGRRLDEYRPARKEPVAGDNVYLTIDFDLQRRAEQYFIENEFVGSAVA
                     LDPRNGEVLAMVSSPAFNPNVYSRRFTPEVWRTIASNPFKVELNRAIQGLYSPGSVFK
                     AVMAMAGLSEGAIDTSTSFFCSGSGVFFGRRFRCYNKNGHGDVSVANALKVSCDIFFF
                     NVGARLGVDRISEYAHKLTFGEISRIDLDGEKAGLVPSTDWATVKQHRKWYPSETISV
                     AIGQGPLLVTPLQVANMMAAISNGGTVYRPHVVKMIEKVDPDGHVERLQVASEVLHKV
                     TLAPRALETVKLGLWKVVNEEGGTGGNARIEGLNVSGKTGTVQVIAQHGWVKTEGLPY
                     KYKDHAWFASFAPKDNPQMVVVVFVEHGGHGGVDAAPLAKLLYESRFREQVLNANLDL
                     SNPDTLQAIKEGQAPVPGQVKKAPAAASGSNPNLGH"
     gene            12328..>13138
                     /locus_tag="VNN25_18695"
     CDS             12328..>13138
                     /locus_tag="VNN25_18695"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93617.1"
                     /translation="MRVYRDDQKFLTFRINALLWAIVIVFVFLAGSFWVVQGVQAEKY
                     RNLSEANALRELVTPAKRGLILDRSGQKILADNQPAYSLSLDRVVMRPIIKNDPSHRD
                     KLVAFLAQVLGVPTQEIEARFEKGKAIPNARPIPVAEDLSMTQVASIQAQAIAFPELD
                     VEPVQRRNYPYATMAAHVMGFIGEANEKDLAAHKELRSGDLLGKRGVEMMYDEYLRGR
                     DGAQYWQYDSHGRRLDEYRPARKEPVAGDNVYLTIDFDLQRRAEQYFIENEF"
BASE COUNT         2664 a         4147 c         3933 g         2394 t
ORIGIN      
        1 cgccggcgat gacacctggc gcgcctacca gcagagtgac atccgcaaga acgagggcgc
       61 acgtcgcgac aacccgccga agacgatcac gtacgtcgcc gggcccgcga cgagcaagaa
      121 gcagtttctc cttcaaaccg agaccggcgt catcgacgca atgagcgctc ccgaggagca
      181 ggccatcgcg gctctggccc aggcgctggg actgaagtga tgtcgacgaa tccgttcccc
      241 ggcctgcggc cattccagga ggaagaggaa cacctctttt tcggacgcga atcgcaggtc
      301 gacgccatgg tcgacacgct tgccgcatcg cattttcttg ttgtggttgg aacgtccgga
      361 tcggggaagt cgtcgctcgt gaactgcggc ctgcgcccgg cccttcatcg cggaatgatg
      421 gcgcgcgcgg gcacgtcgtg gcgcatcgcc cagttccggc cggggaacaa tccgatctgc
      481 gcgatggcgg aagcgctggc gaaagagggt gcgctctaca gcgatgcggc ggaaggtcct
      541 ttcagccaaa gcgagatcat cgagtcgagc cttcgcatga gcaaacttgg gttgctggat
      601 gttttccagc aggcccgcct gccccaggga acgaatctgc tactggtggc tgaccagttc
      661 gaagagcttt ttcgatttca ggagcagagc gcaatcggaa ccaacgcgcg caacgatctc
      721 gccgatcagg ccgcggcgtt cgtcaacctc ctcctggcag tgcgcgagca taaggaccag
      781 cccatctacg tcacgctcac gatgcgctcc gattttctcg gcgagtgcgc gcagttctac
      841 ggactgccgg aggcgatcaa cggcggccag tacctcgtgc ctcggatgac tcgtgacgag
      901 cgcagacgcg ccatcgccgg tcctgtcgga gtgggcggcg cggagatcga tccggtcctg
      961 ctcacgcgtc tggtcaacga cgtcggcgcg aatcccgatc agctttcgat cctgcagcat
     1021 gctctcaacc gcacctggcg gcagtgggaa cgcgagggca gcaaagggcc gctgaccctg
     1081 cagcactacg agaagatcgg cacgatggcc ggcgcgctgg atcagcacgc ggaaaaggcc
     1141 tatgcggagc taagcacgcc gagggagcgg gaaatctgcg agaagctctt caaggcgatt
     1201 accgacaagg ccagcgatgc ccgcggcgtg cggcgaccta cgaaggtgga cacactctgc
     1261 aaactgacgg gtgccacgat cgaagagctg accgctgtcg tcgacgtctt ccgtaagccc
     1321 agccgctcct tcctcatgcc tccattcggc gaggctctga aggccgatac ggtcattgat
     1381 atctcccacg agagcctgat gcgcgtgtgg gaccgcctgc gggtctggac cgaagaggag
     1441 gcacgctcgg cgggaacgta ccaccggttg agcgagaccg cttcactgga ggccgaggga
     1501 aaagcgagtc tctggaccgg cccggacctg gcgggagcgc tggcctggct gaacgacgaa
     1561 aaaccggaag cctgctgggc agagaggtac gcaccaggct tcaacgaggc gatggcgttt
     1621 cttaagaaaa gcgcggacgc gcaagccgcc gagcaaaagc gccaactcga tgaggtgttg
     1681 gagaaggctc ggacccaggc tcgctatgcc cgggcgattg cgttgctttc cgtggccctg
     1741 attctcgcat tggttctcat cggaatcatc tatcatcgaa gcgtcaccac ggcgcacgag
     1801 gcggaaaaca agaagcacaa agagtccgaa gagtcccttc agaaacaggt cgttacctcg
     1861 caggtcgtcg cgcttcaggc gttcgcgatt gacaagcaga agccagagga tgcacgaaaa
     1921 gttcgccaga gcttcgaggc tcttgcccat atctccgcga tcacggcaac cacttccccg
     1981 gagcaacact ccaacacgag gatccagtac ttcaagaagg cgacggactc accgaaactc
     2041 gtcaacgcac tcaaggacct gggcttcgag gacgtgagcg agagaaccgc gagcaatccc
     2101 cgtgagacga atggcatctg gtacggagcc cgcgtgccgg agaacgatgt caagctcgtc
     2161 gcgtttgcgc tcatgcgcgc cggcatcgat cttcagagta ttcagccttc cctccgcatc
     2221 gggcccgagg taatccaggt cggccataac ccctgggttc aagaccaacc cccattcaca
     2281 ccggacgtcg tcagcaagac accgctcggc caactccgcc gcccggaagc acgaacaaag
     2341 gcggatgtaa tcggcacgat caccaaactt gatccgaagg cgcgggaggg atggatcaag
     2401 gggccggaag gcccggtgta cttccagttc tcacccgatc cgccgccgaa gccattcaac
     2461 gtaggtgatg ccgtgacatt cacggtctac tacggaaagc gaatgtacgc ggagggggtc
     2521 acggcacgcc cggcaacacc ggggcctcaa tcgttacgcc gcggtagtta gttagctgtg
     2581 ctgctgcaca ctcgcggggg attggcggcg gcgcggtgct gcaaggtctg gagagtgcga
     2641 accgaaattg gtcctgaccc aagttcagga gccaaaacga ctaccgaaat tggtcccgac
     2701 ctagtttcac gagccagaac gactaccgaa attggtccca acccaatttc aggtgtcaaa
     2761 atgaccaccg aaattggtcc cggcctaatt tcagtagtca aaacgaccac cgaaattggt
     2821 cccgacctaa tttcagtagt caaaacgact accgaaattg gtcccgaccc aatttcagga
     2881 aggtcaagag cctaccgaaa ttggtcccga cctcatttca ggaaggtcaa aagcctaccg
     2941 aaattggtcc cgacccaatt tcaggagtca aaacgactac cgaaattggt cccgacctaa
     3001 tttcacgagt cgaaaccagt accgaaattg gtcccgaccc aatttcaggg atcgaaggaa
     3061 gtggtcaaat cgcctcaacg gctcagcgcg ttggcgacac gctcgatcgg caccggcttc
     3121 ttgtcctcgc ccacgttcac gtagaccacc tgcgcctcgg tgacgaggac tttcttctgc
     3181 ggatcagtgc ctcgaatcgc ttcgcagacg actttgaccg tgatcgaggt tctaccgatg
     3241 cggagggtct cggtccagaa gctgatgagg tcaccgacga agaccggctg gtgaaagacg
     3301 atcttgtcca tcgacacggt cagcatcagc cgcgagccct ggcggcgtgc ttcgatcgca
     3361 ccggcctggt cgatgtagct gaggatcacg ccaccgaaga tcgtgccctg cgcgttcgtg
     3421 tcgcgcggca tcatcaacac ccggatggcg ggctcggccc aacgttcggc gatttcagtc
     3481 atgttcgatc acaccgggcg tcggttatcg ggggtcggaa gtccgagaat caactggcga
     3541 agctttcgct ccgacccccg actcccgata accgactccc ggtttcgacc tgaccccttc
     3601 ctgcctagct ggactgctgc gaaggcgtct gctgttcggt gaccttcttg ttgatgtcga
     3661 tcgatgctgc tttgcgcagg acctggagca tcgtggcgcg gaggttccgt gactcctgct
     3721 gccgcaagtt ctccatgtac tggccacggt tctgcgcgag cgtggcggga tcaaccttct
     3781 tctgctcgag gacctggaag atcacggcac cgtcgccggt catgaccggg cccttcacct
     3841 gacccacggg agcggtcatc gccgcattga cgagcgccga cgtatcgccg gtgaacccgg
     3901 tcacgaagcc ctgccggttg accgaggttt ccgcggaggc aaggccgagc ttggtggcgg
     3961 ccagatccac ggtcggggca ggcagctcct tcgccagcgc ctgctgcccg agcatccgcg
     4021 ccttctccat ctttgcgtcg ttctcaacct tggcgcgcac ttcgtcgagc ggcgaaacgc
     4081 cggccgagcg gatgttgtag aggaacggaa tggccggacc gcgctgcgtg ccgatgatgt
     4141 cgccgacgtc gttctgcttg gccgcgaagg cccagctcgc gaacgccgga ttgtttccca
     4201 ttcccggaat cggatcggcc ttgccgaacc actgcgtgtc attcgaagcc acgtccccgc
     4261 tggccagcgc cgtgaactcg ggggcactct tcggcttgct ctctttgagg cgcgcggcga
     4321 tgctggtgat ctcatcgcgg gcgcggtcct tcgcctgctg atcgatcgtc aagccacgca
     4381 accggtcgcg cacttcttcg aacgtgtggt atcccggcgg acgccgctcc agcactttga
     4441 tgatgtggta gccaaactcg acggagtgga tcggatcgct gatcgtgttc agcggcatcg
     4501 tgaaggcggc cttgtcgaac ggctcgaccg tcgtgccctt gtcgacccag cccatgtcac
     4561 cgcccttgcc agcggaggag ggatcgcctg agttttcttt cgccagcttt ccgaagtctg
     4621 cgcctgcgcg aagttggcga acgatgtcgt cggccttggc tttggcggcc tgatcgaccg
     4681 gcgcgggagc accctgatcg accttgatga ggatatgaag aatgtgtgcg ccctcgggag
     4741 ccttgaactc ctcatggcgc tggtcgtaaa tcttgtgcac ctcggcgtcg ctgggattga
     4801 tcgatgcccg caccttggcg tagtcggcga tcaggtactt ggctgcgcgc tgttcgccgt
     4861 gcgtgtattg cgactggtgg gtggtgtagt actgctgcac ctcggccggc gtgacggtga
     4921 cattggccgc ttccttggcc gcgggataga ggacgtactt gatcctcgcg gtttcgctgg
     4981 tgcggcgata ctcgtcgtcg gctgacttcg gcgagatgat gatcgagttc tgaatggcgc
     5041 tctccatctt cgcgagcgtg atctcgcggg caagctcctc ttcgaactcg gccggtgact
     5101 ggtaacccat catcgttacg tagcgcgtgt agatctccgc gccgacaaac ttgccgtcgg
     5161 gattgagcgt gggcagatcc agaatcttct tccgcacttc ctcggctgat gcactgaggt
     5221 gaagacggcg ggcctgctgg aggagaagat gctgatcgat cagcgagtcg agcacctgct
     5281 tctgcagccc caactgttga acctggtccg gcgtgagcgg ctgatggtag acctgctcgt
     5341 agttcttttc ggtgtagtaa agagcgcgat cgaactcgcg gtacgagatc gtctccccgt
     5401 tcacgcgggc ggcgtatgca cgctggtcga ttccgccacc gttgccggca ccgccagcgc
     5461 cccagtcgac gaagatgaag aggatgaaga ccgcaacaat ggcgaccagg atccatttga
     5521 ggtgctgaaa gctatcgcgc atcaacttga gcatcgggac gaacctctgt tttgacttga
     5581 tttggatggc aaccgcggcg cgggacacct ccagccgggc gatcgcgtga atccgtaaaa
     5641 aacgcggcag tgtagctatt cccgcctccc ggagcaaact gtgcctctgg gcgtgctata
     5701 aacttcgact cgtctcgatg gctaaaaaga agaagcagag ctccgaaccc gcgatcgatt
     5761 ggtatctggt ttccatcgag cggctcaagc agatcgggat cgtgattctc gtcgtgcttc
     5821 tcggcggcgg agcgtggtgg tactacacca accagaaacg taatccgcgc agctcggctg
     5881 aagcggcgat cgccgaggcc aggcaggcgc tcaacacact cgcagcgtcc aaagactttc
     5941 cgatgcatcg cagcgatttc gatcgcgcgc agcacaagct cgacgaagcc gggacgaagc
     6001 tcgccgccgg caactacccc gacgccgaga gcgccgcggt ggagtcgcag accttcagca
     6061 gggccgcgct cagcggaaaa ggaggcgcgg acaacgacgc gcagttcctc accgtcgaag
     6121 gcgacgtgca gttccagaag tcgtcgacga gcgactggag tgcggcggag acgcgcacac
     6181 ctctcttcaa cggcgactgg gtgaagacgg gacgcagcgc ttccgcggag ctcatctttt
     6241 cgaacggttc gctctacacg atcggaccga acgcgctcct cgagatctac tcgcagttca
     6301 atgccgcgac cagccggaag aacaactccg tgcagatgca ggtcggctcg gtcgaagtag
     6361 cgacgaccga cgacgcttcg agcgtccgca cgcccggcag ccaggtcacc atcgactccg
     6421 agtcgacgac gcaagtcgga gtcgacgagc agaagacgac cgccgtcctt tcggaaaagg
     6481 gatcggcctc gatcgccccg tccgatggca gcgcggcagt gaaactcgcc gtcggcgaca
     6541 aggtcaccgc cacgccgcag ggagcacttt ctgcggtgcg caagcttcct atgcctcccg
     6601 cgctcctcac gccatccgac aaccaggtct ttctggtcac cgccgattcg cgcgtccagt
     6661 tctcgtggga agcggtaccg ggtgccgccg gctacgtcct gcaagtctcc cgttcgcgcc
     6721 ttttcacctc tctggagatc aactcgacac gccagaaaac caccgcctcg gcgcaagtga
     6781 ccgccgaggg tgccttctac tggcgcgtgg cagcaacgga agcgggaggc gacaccggcc
     6841 cgttctccgc attccgacgc ttccgcgtca acggcccggg tcacgaaggg acgccggccc
     6901 tggatgctcc ggcaccacgg ctgctgctca agcgtcccta ctcgattggc ggacccttct
     6961 tcatgatcga aggggacaca gacccgggcg caacggtcct catcaatgac gaagacgccg
     7021 acgtcgaagg aaacggtcac ttcaagaaac tagtcagttt caacaaggtc ggaaccaatc
     7081 tggtcgtcat caaagcaacc aacgcagcag gcaaacaaac ccggcaatcc gagacggtgc
     7141 acgtggaaga ataggttcgc gggagtcgca gatcgggggt cgggagtcgg agagcactgg
     7201 caacgtttgc gctccgactc ccgactcccg actcccgatc cgcgaccaaa gcaataatag
     7261 gcagtggagt gtagggtgtg gggtgtggga gcaaatgagc aattcgcagt catcgtttct
     7321 cccactcccc actccctaca ccccaccccc cgaaaccagg gcaacgctct tgcaccaagc
     7381 ctcccaaacg ttcaaaaagg acaagatcta gaaaatggct ctcaaatcgc tgctctcgat
     7441 gttctcgaac gatctggcga tcgatctggg aacagcgaac acgctcgtct acgcgaagaa
     7501 caaaggcatc gtcgtccgcg aaccctcgat cgtcgcggtc aacaagatca acaacaacgt
     7561 cgaggcggtc ggcaaggaag cgaaggaaat gctcggccgt acgccgggca acatcgtggc
     7621 catccgcccg atgaaggatg gcgtgatcgc cgacttcgaa gttacggaga agatgatcaa
     7681 gtacttcatc gagaaggcac acggccggcg cttcctcgtc aagcctcgca tcgtcatctc
     7741 cgtcccctcg gagatcacgc aggtcgaaaa acgcgccgtc aaagactcgg ccctgcgcgc
     7801 gggcgcgacc gaggtgtatt tgatcgagca ggcgatggcg gcggcgatcg gggccggact
     7861 gccgatcacc gatccgaccg gcaacatgat cgtcgacatc ggcggcggca ccacggacgt
     7921 cgccgtcatc tcgctggcgg gtatcgtcta ctcgcgctcg gtgcgcgtcg ccggcaacga
     7981 gatggacgac gcgatcattc agtacatcaa acgcaagtac aacctcctca tcggcgaacg
     8041 taccgccgag cagatcaaga tggagatcgg ctccgccttc ccgctcgacg aagagatgac
     8101 gatggagatc aagggccgcg atctcgtcga aggcgttccc aaaaccctcg tcgtcagcga
     8161 tgaagagatc cgcgaagcgc tctccgaaac ggtggccacg atcgtcgaag ccgtgcgcgt
     8221 cgcgctcgag cgcacgccgc ccgagctctc cgccgacatc atggacaagg gcatcatcat
     8281 cgccggcggc ggatcgatgc tcaagaatct cgacaagcgt ttgcgcgaag agaccggcct
     8341 tcccgtgacg ctggcggaag atcctctggc ctgcgtagca ctcggcaccg gccagatgct
     8401 gaccgacttc aacctgctgc ggaagatttc gattgagtaa cggggagtgg ggagttgggg
     8461 gtggggagtg ggagtcaaac ggcgatcgac cttcgtttca ccgacagcct tcttggagca
     8521 ccgggggtcg ggagtcggga gtcgaagagc gccgaagatc agagctcatc acgaatccgg
     8581 ctccgtttct ctcccacacc ccacccccca ctccccacac cctctcccca cacccatggc
     8641 ccagaatctg ctccataacg cgcacgaacc atggccgcac ccgctgacgt aatcccacgg
     8701 cgcccgacgc tgctgttcat cgtcgtcctg gcgctgctct tttttctcat gagcctgagc
     8761 agcaggacgc gctacatcgg cgagacccgc acgatgttcg agcgcagcgt catgatcctc
     8821 ttcgcgccga tcccgaagac agtgaactgg gccggccagt cgatgtcgga catctaccac
     8881 ggctatctcg acatgcggcg ctcggtcggt gagaacctgc agcttcaccg gcagatcgat
     8941 gagctgacga ccgaaaacct tcgccttcgc cagtccgaag gcgacctgcg ccgcctgcgc
     9001 agcctcctcg gctattcgga agagttcacg atgccgacga cgatggccca accggtcatg
     9061 ctcgacatct ccgggcgctt caaatcgatc atcctcgacc gcggatcgaa cagcggcatc
     9121 gaggtcaacg acgccgtcgt caatgccaac gggctgattg gccgcgttgt attgacgacg
     9181 aaggacatgg ccaaagtgca attgatcacc gacggaaact cctcggtcgg cgttctcatc
     9241 gaccggaccc gccggcaggg cgtcctgcgc ggtgatagcg cggcaggcac gcagctatac
     9301 gacatccctt cgctggccga cgtcaggctc ggcgacgtcg tcatgaccgc gggcatcgac
     9361 ggcatctatc cgaaaggaat cccggtcgga gtcgtgacaa aggcggagaa ggggcaggac
     9421 ctcttcaaga ccatcgtcgt ccgtccctcc atcgacttcg gctcgatcga ggaagtaatc
     9481 gttctgcaca cacgcaaaat cccgaatgat gtggtgaggt acacaccttg aggtggcttc
     9541 gattcgcggt cggactcctg atcgctctga cgctccattc gctgctcgac agcttcttcc
     9601 cgaacgcgct ggcgttcttc aacccgtacg tcatcctcgt cgtctactac tcgatgggcg
     9661 gcaacgtctt cggcgcgatc atcgccggcg tcatcgcggg gctggtgcag gacgcctact
     9721 ccggcgcgat gttcggactc cacgccttcg ccctcaccgt ttgcgcctac gccgtggcct
     9781 acatcaactc ccgcctcgtg ctgcgcggca cgctggcgtt cggcgcctgc ctgatcggcg
     9841 ccgtgatcgt gaacgagttg gtgatctaca tcctcgtgaa catcctcctc aaacagaaag
     9901 tggagatgtt cgaacaggga atgatcgtga agacggtctt cacctgtctc ttcggcatga
     9961 tcgtctatct attgctgacc ctgtttctgc gggaagagcc gatcgacgca acgaggaggc
    10021 cgacagggtg gtagttttgc gcgggggtcg gttttcgggg gtcgggggtc ggagcaaggc
    10081 ctgctatatg caagaccgat cgccgacaac cgatacccga taatagagat cgacccactc
    10141 cgacacccga cacccgactc ccgaccccct gcaatcaaac catgcgcgtc taccgagatg
    10201 accagaagtt cctgaccttc cgcatcaatg cgctcctctg ggcgatcgtg atcgtgttcg
    10261 tgtttctggc gggctcgttc tgggtcgtgc agggcgtgca ggcggagaag tatcggaacc
    10321 tttcggaggc caacgccctt cgtgagctgg tcacgccggc gaaacgcgga ctgatcctcg
    10381 accgcagcgg gcagaaaatt ctcgccgaca accagccggc ctactccctg tcgctcgatc
    10441 gcgtggtcat gcgtccgatc atcaagaacg atccgtcgca tcgcgacaag ctggtggcat
    10501 tcctcgctca ggttcttggc gtgcccacgc aggagatcga ggcccgattc gaaaaaggga
    10561 aggccattcc taacgcgcgg cccattccgg tcgcggaaga tctgtcgatg acgcaggtcg
    10621 cctcgatcca ggcccaggcg attgcgtttc ccgagctcga tgtcgagccg gtgcagcgcc
    10681 gcaactatcc ctacgcgacc atggccgcgc acgtgatggg tttcatcggc gaggcgaacg
    10741 agaaggacct cgctgcgcac aaagaactgc ggtcgggcga tctcctcggc aaacgcggcg
    10801 tcgaaatgat gtatgacgag tatctgcgcg gccgcgacgg cgcgcaatat tggcagtacg
    10861 acagtcacgg ccggcgcctc gatgagtacc gccccgcgcg caaagaaccg gtggccggcg
    10921 acaacgtcta cctcaccatc gatttcgatc tgcagcggcg cgccgagcag tacttcatcg
    10981 agaacgagtt cgtcggctcc gcggtggcgc tcgatccgcg caacggcgag gtcctggcca
    11041 tggtctcctc gcccgccttc aaccccaatg tctactcgcg ccgcttcacg ccggaagtct
    11101 ggcgaacgat cgcgtcgaat ccgttcaagg tcgagctgaa tcgcgccatc cagggtctct
    11161 attcgccggg atccgtcttc aaagcggtca tggccatggc gggtctgtcc gaaggtgcga
    11221 tcgatacgtc gacatccttc ttctgcagcg gcagcggcgt gtttttcgga cgccgtttcc
    11281 gttgctacaa caagaatggc cacggcgacg tgagcgtcgc caacgctctg aaagtctcgt
    11341 gcgacatctt cttcttcaac gtcggcgcgc ggctcggtgt cgacaggatc tctgaatacg
    11401 cgcacaagct caccttcggc gaaatctctc gcatcgatct cgacggcgag aaggccggcc
    11461 tcgttccctc cacggattgg gcgacggtta aacagcaccg caagtggtat ccgtcggaga
    11521 cgatctcggt cgccatcggt caggggccgc tcctcgtcac gccacttcag gtcgcaaaca
    11581 tgatggcggc catctcgaac ggcggtacgg tctaccgccc ccacgtcgta aagatgatcg
    11641 agaaggtcga tcccgacggc cacgtcgagc gcctgcaggt cgcgagcgaa gtgctgcaca
    11701 aggtcacgct ggctccaaga gcactcgaga ccgtcaagct cggtctctgg aaagtcgtca
    11761 acgaagaagg cggcaccggc ggcaacgccc gcatcgaagg tttgaacgtc tccggcaaga
    11821 ccggcacggt ccaggtcatc gcgcagcatg gctgggtgaa gaccgaaggg ttgccctaca
    11881 aatacaagga tcacgcgtgg ttcgcgtcgt tcgcgccgaa agacaatccg cagatggtcg
    11941 tcgtcgtctt cgtcgagcac ggaggccacg gcggtgtcga cgccgcgccg ctggccaagc
    12001 tgttgtacga atcgcgcttc cgcgagcagg tcctgaacgc caacctcgat ctctcgaacc
    12061 ccgacacgct tcaggccatc aaggaaggac aagcgcccgt ccccggccag gtcaagaaag
    12121 cgcccgcggc agcatcaggc tcgaacccga acctcggcca ttgattttgg gcggaaaacc
    12181 tcaccacaga ggcacagagg tcacagagat ccctctgtgt tctctgtgtc tctgtggttg
    12241 cctttgcttc ggcaaccaca ggaccgatga ccgataatag agattaattc cgctccgaca
    12301 cccgacaccc gactcccgca acaaaccatg cgcgtctacc gagatgacca gaagttcctg
    12361 actttccgca tcaatgcgct cctttgggcg atcgtgatcg tgttcgtgtt tctggcgggc
    12421 tcgttctggg tcgtgcaggg cgtgcaggcg gagaagtatc ggaacctttc ggaggccaac
    12481 gcccttcgcg agctcgtcac gccggcgaag cgcggtctga tcctcgaccg cagcggacag
    12541 aaaatcctcg ccgacaacca gccggcctac tccctgtcgc tcgatcgcgt ggtcatgcgt
    12601 ccgatcatca agaacgatcc gtcgcatcgc gacaagctgg tggcattcct cgctcaggtt
    12661 ctcggcgtgc ccacgcagga gatcgaggcc cgattcgaaa aagggaaagc cattcccaac
    12721 gcgcggccca ttcccgtcgc ggaagatctg tcgatgacac aggtcgcttc gatccaggcc
    12781 caggccatcg cgtttcccga gctcgacgtc gagccggtgc agcgccgcaa ctatccgtac
    12841 gcgaccatgg ccgcgcacgt gatgggcttc atcggcgagg ccaacgaaaa agacctcgcc
    12901 gctcacaaag aactgcggtc gggcgatctc ctcggcaaac gcggcgtcga gatgatgtat
    12961 gacgagtatt tgcgcggccg cgacggcgcg cagtactggc aatacgacag ccacggccgg
    13021 cgcctcgatg agtaccgccc cgcgcgcaaa gaaccggtgg ccggcgacaa cgtctacctc
    13081 accatcgatt tcgatctgca gcggcgcgcc gagcagtact tcatcgagaa cgagttcg
//
LOCUS       DATLVW010000641         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_236930, whole genome shotgun sequence.
ACCESSION   DATLVW010000641 DATLVW010000000
VERSION     DATLVW010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236930"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..516
                     /locus_tag="VNN25_18700"
     CDS             112..516
                     /locus_tag="VNN25_18700"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH93618.1"
                     /translation="MIVFIDSNIPMYVAGREHPNREPSLRFLEAVRRNDVEGCTSTEV
                     LQEILYRYSALGRLDVAREVYNFFVAMCPMIFDVTLTDTNMARDLLMKHRGISARDAV
                     HAAVMLNHDIELIATFDSDFDRVAGIRRLALP"
     gene            648..1283
                     /locus_tag="VNN25_18705"
     CDS             648..1283
                     /locus_tag="VNN25_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXH93619.1"
                     /translation="MAKSKEEIVQEFRVQSIQEAAIRVISRKGMAAATMQEIAEEAGV
                     AKGTIYLYFRDREELVEKTFETAIGQLHGRLDAALDAEGPFAEKLRSLLTAQFAFFGE
                     NREFFRLYHSLRMTQGSATQQRRQKRTCQPQFKARVDRLAGMLKAAMDAGEVRVLDPS
                     RLALFLIEGSIAIVLERLTEDAPPPESEDVELIVSTLLGGIAIPKPKRSSH"
     gene            1280..2575
                     /locus_tag="VNN25_18710"
     CDS             1280..2575
                     /locus_tag="VNN25_18710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH93620.1"
                     /translation="MKRAVLTLILLASSATAGAQTKLTLPEALERALRVNNTVERSRI
                     DATVADQNRKLLLSSILPQVQFTGSAIRNSTEVAFGTGADARTVLPGNDWNYRVVLSQ
                     PVYAGLREKRAYDQAKIGTVNARLGVHETEDAILLRVASNYLGVANADANIAIEQRNI
                     GLAEKRRSQSNAFYQAGEVTKVDVLRAETAIKSSQRLLATAQQQREAAVSGLREDLDL
                     TGDIAVTPPDNALAPLPDEQTLMTRAESSRPDVAVAQNNIRIAELEVLKQRGGRLPVV
                     TFDAGYIDQKAAFPASRYGYGAFRFSVPIWDSNQVNLRVATAKEREEQAKLLLEDAKT
                     AAHEDVRKAITDLHSADTTLQLAKEQLAAADAEYAQAFELYRAQESTSLDLATSELSL
                     ADARRAVASETLNHNLAQLRVWYAAGAIKEAFGVGATQQ"
     gene            2572..>2805
                     /locus_tag="VNN25_18715"
     CDS             2572..>2805
                     /locus_tag="VNN25_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93621.1"
                     /translation="MTRSFISTISILILAILPLGCEKKDAAPATTTQAAPALPGDVQN
                     VASAATATTATTTASDSASAASSGIASSESGGTI"
BASE COUNT          564 a          878 c          853 g          510 t
ORIGIN      
        1 gatgagcgat cgatcaccct cgattgatga tcctctcgcg cggctcgcgg ccttgaacgc
       61 gccgaccggt gacattcagc agatgttggc cgagatcgcg gccggacgcc ggtgatcgtc
      121 tttatcgatt cgaacattcc gatgtacgtc gcgggacgtg agcatcccaa ccgcgagcct
      181 tcgcttcgat ttctcgaagc cgttcgccgg aacgatgtcg aaggctgcac cagcacagag
      241 gtgctccagg agatcctcta tcgctattcc gcgttgggaa ggctcgatgt cgcacgggag
      301 gtttacaact tcttcgtcgc gatgtgtccc atgatcttcg acgtcacgct aacggatacg
      361 aacatggctc gcgacctcct gatgaagcat cggggaatct ccgcgcgtga cgcggtgcat
      421 gccgccgtga tgctgaatca cgacattgaa ttgattgcca catttgattc tgactttgat
      481 cgtgtggcag gcattcgtcg tctcgcgctc ccgtagttcg gccggcgcct gtgacgcatc
      541 ggtaaatccg agcactcatc ccttgcctct gacccggaat tctggttgac tgaccgttcg
      601 ttcagcgcga tggatacgct gtcagctcgc tgggcgatcc ccaaaacatg gccaaatcca
      661 aagaagaaat cgttcaggaa ttccgcgtcc agtccatcca ggaggcggcc attcgcgtga
      721 tctcacgcaa gggaatggct gcggcgacga tgcaggagat cgccgaagag gcgggcgtcg
      781 caaaggggac catttacctc tacttccgag accgcgaaga gctcgtcgag aagaccttcg
      841 agaccgcgat cggccagttg cacggacgac tcgatgccgc cctcgatgcg gaagggccgt
      901 tcgcggaaaa gctgcggtcg ctgttgaccg cgcagtttgc gttcttcggt gagaaccgcg
      961 agttcttccg gctctatcac tcgctgcgca tgactcaggg gagcgcaacg cagcagcggc
     1021 ggcagaagcg tacgtgtcag ccgcaattca aagcgcgcgt cgaccggctc gcaggcatgc
     1081 ttaaggcggc catggacgcg ggcgaagtgc gcgtgctcga tccatcgcgg ctggcgcttt
     1141 ttctgatcga agggagcatc gccatcgttc tcgaacggct gaccgaggat gcgccgccgc
     1201 cggagagcga ggatgttgaa ctgatcgtct ccacgctgct cggcggcatc gccattccga
     1261 aaccgaaacg gagctcacat tgaaacgtgc ggtactgaca ttgattctgc tggcgtcgtc
     1321 cgcgaccgcc ggcgcgcaaa cgaaactgac gttgccggaa gcgctggagc gcgccctgcg
     1381 tgtcaacaac acggtcgagc gctcgcgcat cgatgcgaca gtcgcagacc agaaccgcaa
     1441 gcttctgttg tcgtcgatcc tgccgcaggt gcagttcacc ggttcggcga tccgcaactc
     1501 caccgaggtc gcgttcggca ccggtgccga cgcgcgcacc gttctgccgg gcaacgactg
     1561 gaactaccgc gtcgtgcttt cacaaccggt gtatgccggt ttgcgcgaga agcgtgcata
     1621 cgatcaggcc aagatcggca ccgtcaacgc gaggctcggc gttcacgaga ccgaggatgc
     1681 catcctcctt cgcgtggcct cgaactacct cggcgtcgcc aacgccgacg cgaacatcgc
     1741 gatcgaacag cggaacattg gtctcgccga aaagcggcgc tcgcagtcga acgcgttcta
     1801 tcaagcgggc gaggtgacga aggtcgatgt actgcgcgcc gagacggcga tcaagtcatc
     1861 gcagcgattg ctggcaaccg cgcagcagca gcgcgaagca gccgtcagcg gattgcgcga
     1921 ggacctcgat ctgacaggag acatcgccgt gacgccaccg gacaacgcgc tggctccgct
     1981 gcctgacgag cagacgttga tgacccgcgc cgaatcgtcg cggcccgatg ttgccgtggc
     2041 ccagaacaac atccgtatcg ccgagctcga agtgctcaaa cagcgcggcg gccgtctgcc
     2101 agtcgtcacg ttcgacgccg gctatatcga tcagaaagcg gcgtttccgg ccagccgcta
     2161 cggatatggc gcgttccgtt tcagcgttcc gatctgggac tcgaaccagg tcaatctgcg
     2221 cgtggccacg gcgaaagagc gggaggaaca ggccaaactc ctgttagaag acgcgaagac
     2281 cgccgcgcat gaagacgtgc gcaaggccat caccgatctg cactccgccg atacgacgct
     2341 gcagttggcg aaggaacagc tcgccgctgc ggacgccgag tacgcccaag cgttcgagct
     2401 gtatcgcgcg caggaatcga cctcgctcga tctcgccacg tcggagcttt ctctcgctga
     2461 tgcgcgccgc gccgtcgctt ccgaaacgtt gaatcacaac ctcgcacaac tccgcgtctg
     2521 gtatgccgcc ggcgcgatca aagaagcttt tggagtggga gcaacccagc aatgacgcgt
     2581 tcattcatca gtacgatttc gattttgatt ctggcgatcc ttccgctcgg ctgcgagaag
     2641 aaggacgctg cccccgcgac aacgacgcag gccgcgccgg cgttgccggg cgatgtgcag
     2701 aatgttgcat cggcagcaac agcgacgacc gcaacgacaa ccgcaagcga ctccgccagc
     2761 gcagcatcct caggcatcgc gtcgtccgaa tcgggaggga ccatc
//
LOCUS       DATLVW010000642         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_276784, whole genome shotgun sequence.
ACCESSION   DATLVW010000642 DATLVW010000000
VERSION     DATLVW010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276784"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VNN25_18720"
     CDS             complement(<1..525)
                     /locus_tag="VNN25_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXH93622.1"
                     /translation="MTTNPTDVVLYVRVSSREQAVEGFSIEAQRRFLLQHAAVANLRI
                     VAEFNDEETAKTPGRSGFTAMMTYLRAHPLVRTVIVEKTDRLLRNWKDFITIDDAAID
                     VVFVKEGTTFGPNSHSSQRFLQSIKVGMARHYSENLSEEVKKGLHEKCRLDGAWPAKA
                     HIGYINCGAPYGIDP"
     gene            complement(850..1749)
                     /locus_tag="VNN25_18725"
     CDS             complement(850..1749)
                     /locus_tag="VNN25_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93623.1"
                     /translation="MSSQRAAAVDGLAKLGAIILTLGNLLLAAIVAAETAVRLHAEFC
                     ESNKAALDDWRTKQASFAGFVLKVLFAFLADLFLLHPLLVALALTMFSFSAIGLLASL
                     ITPILFIVAESIIAVKYERARQWEARFGVKGHAMRWLILGYVVAAIPAMIVIALGFVS
                     SGFIASLGLVAFLGRQALNVILAIISFVMHILLVHSGMTPREFVDSLLAKLGKQRPER
                     REGGALNDARRKRTALLQAASRYEHARLDEQERHGPIAPYPFPESILALIREELPNFG
                     VVGPQAVAPLDAPDKQRPLFPVN"
     gene            complement(1742..>2218)
                     /locus_tag="VNN25_18730"
     CDS             complement(1742..>2218)
                     /locus_tag="VNN25_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93624.1"
                     /translation="LDPEPARPKNADLTGNALIDMDRAADHARVNEEYEKHHQAWAAR
                     TDAAVEAFRRSAESLLAQSADAPATDLVTAIERAYVALDEPTPFPWLAKAPRTLVVIT
                     DGADTVTRRRVPAAGFPVVLIIVNGNGLTGDLGHLHPLRFESVDSMLTYVTGGRHV"
BASE COUNT          424 a          669 c          716 g          409 t
ORIGIN      
        1 aggatcgatg ccgtacggcg cgccacagtt gatgtagccg atgtgtgcct tcgccggcca
       61 cgcaccatcg aggcggcatt tctcgtgcag ccctttcttc acttcctcgc tcaggttttc
      121 gctgtagtgc cgcgccatcc cgaccttgat cgactgcaag aagcgctgcg acgaatggct
      181 gttcggtccg aaggttgtgc cttccttcac gaacactacg tcgatcgcgg catcatcgat
      241 ggtgatgaag tctttccagt tgcgcagcag ccggtcggtc ttttcgacga tgaccgtccg
      301 cacgagcggg tgcgcgcgca gataggtcat catggccgtg aagccggagc ggcccggggt
      361 cttcgccgtc tcctcgtcgt tgaactcggc gacgatccgc aagttcgcga ctgccgcatg
      421 ttggaggagg aagcgtcgct gcgcctcgat cgagaagccc tccacggctt gctcgcggct
      481 ggacacgcga acatagagaa ccacatccgt cggattcgtc gtcattccct caacctccgc
      541 agcgaagctg gagaggtatt gtcctgcaag gaatcgcttc gcgcatgcag ttcttgccag
      601 cgcgcgagga gtgcgaagag atcgagcagg cgtcctccgg ctgcagccca ttcatcgtcg
      661 ctaatctccc tccgtagagt cgatcaaagc tcgcgcgtgc cgtagcgtgc agctgatcgg
      721 cagacgtgcg aaccgttcgt ttcgacgcgc tcttcggcat ccactgcggc gcacgaatcc
      781 agtcgtgcgt cccggtggcg gtcgaaagtg agggtcgctt gacagcagcg accctcgttc
      841 gatcaagcat caattgaccg gaaaaagcgg gcgctgtttg tccggggcat cgagcggtgc
      901 cacggcctgg ggtccaacca cgccaaagtt cggcagctct tcgcggatga gcgcgaggat
      961 gctttcgggg aacggatacg gggcaatcgg cccatggcgt tcctgctcat cgagacgtgc
     1021 gtgttcgtat cgcgatgcgg cctgcaggag cgcggtcctc tttcgccgcg cgtcgttcag
     1081 cgcgccgccc tctcgacgtt ctggtcgctg tttcccaagc ttcgcgagga gggaatccac
     1141 aaattcgcgc ggagtcatgc cgctatgcac cagcaggatg tgcatcacga acgaaatgat
     1201 cgcgaggatg acgttcagcg cctggcggcc gaggaacgcg acaaggccga gcgaggcgat
     1261 gaagccagag gagacaaacc ccagcgcgat gacgatcatc gcgggaatcg ccgcgacgac
     1321 gtagccgaga atgagccaac gcatcgcatg gcctttgacg ccgaagcgtg cctcccactg
     1381 ccgcgcgcgt tcgtacttca ctgcgatgat cgactcggcg acgatgaaga gaatgggcgt
     1441 gatgagcgag gcgaggagcc cgatcgccga gaagctgaac atcgtgagcg cgagcgcgac
     1501 aagcagcggg tgcaggagga agagatcggc aaggaaggcg aagagcacct tcagaacgaa
     1561 gcccgcgaag ctcgcctgct tcgtgcgcca atcgtccaac gcggccttgt tgctctcaca
     1621 gaattccgcg tgcaggcgca cggcagtttc ggccgcgacg atggcggcga ggagcagatt
     1681 cccgagggtc agaatgatgg caccgagttt ggccaagccg tcgacggcgg ccgctctttg
     1741 gctagacatg gcgacctccc gtcacgtagg tcagcatgga gtcgaccgac tcgaagcgaa
     1801 gtggatggag atgaccgaga tcgccggtca ggccattgcc attcacgatg atgagcacga
     1861 cgggaaaacc tgctgcggga acgcgccgac gcgtcaccgt atcggcaccg tcggtgatga
     1921 cgacaagcgt ccgaggagct ttcgccagcc agggaaacgg cgttggttca tcgagcgcga
     1981 catacgcccg ctcgatcgct gtgacgagat cggtcgcggg ggcatccgct gactgcgcga
     2041 ggagcgattc cgcagaacgg cgaaatgcct cgaccgcagc gtcggtgcgc gctgcccatg
     2101 cctggtgatg tttctcatac tcctcgttca cgcgtgcatg gtcggccgcc cgatccatgt
     2161 cgatgagtgc attgccggtg agatcggcgt tcttcggtct ggccggttcc ggatcaag
//
LOCUS       DATLVW010000643         5802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_480830, whole genome shotgun sequence.
ACCESSION   DATLVW010000643 DATLVW010000000
VERSION     DATLVW010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480830"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VNN25_18735"
     CDS             <1..1156
                     /locus_tag="VNN25_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93625.1"
                     /translation="AFAASKEIADDLRRAHFHHGAFYLLNTIQLSDIGYDSSFYVPTA
                     DRQSGFRFGIQAPTRLYIVPSKKTVYSIEVRPEWSFFSRGSSKAVFGYQGRADAQFLL
                     NHLYLDFYAQSANQLRADVSEIARLLTEKASATGVSGELKYSSRTSMTFNASAGKSSH
                     PLDKIQPDAPVQLLDRSEHNYRFTVHHKTFPLTSLFVATEFSDYSFTDAVFKNGRRTY
                     AGFGLVHDTGRTVTQGEVGEGKLDFIRPGQHDFQGALGNLSSSKKFGNFTTGRLTVSR
                     DVDFSIFALNNYYIADRVSGLLAWDATRRLTLNAQASLGRDLYDTPVIGPNLVLLKRR
                     DVFSFPSVGFLYAFARLRGGADIGYVRRTSNFNVNLDHGIRVLFRLSFTP"
     gene            1344..2228
                     /locus_tag="VNN25_18740"
     CDS             1344..2228
                     /locus_tag="VNN25_18740"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXH93626.1"
                     /translation="MKRVLLAAAAIGMLTIPAFSQRPPTPQPQIPAGSLLAEPPASAS
                     DAPIGPRDQIEVKVYQDPNLYTRATVSDDGRITMPLIGKVDVSGLTPTEVEQRIKALL
                     EAKYINKADVTVTVTEAGSKPISVIGAVMRPGRIGITGNITLIQAITQAGGLGTGYGR
                     TLYVLRTASNGLTEQIAVDIDDLMVNGNPDLNLPLRANDVINVPIDSSISIYVLGEVM
                     HPGPAQFRRSQTPTLLQVLASAGGPTDRASKRVVLKRMVGGTEKTFHYDFRKIIDGRQ
                     NDIVLMDGDRLIFQESFF"
     gene            2307..4520
                     /locus_tag="VNN25_18745"
     CDS             2307..4520
                     /locus_tag="VNN25_18745"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HXH93627.1"
                     /translation="MDLNTQEVHLSHYWNIIYKRWKVALSILVVVMLGTYVASYFAKP
                     LYRSTIQIQIERENPSQLTVDDLFGIAASDQEFLQTQYVLLKSRGLAEKIIDDQKLLS
                     DPSFYPAGINGKTAAQIAQARESMVGAVIASIEVNPVRATSLVEIGCIAATPQLAQKI
                     AEGVGESYIRLNNEKKFESVQKASDFLDRQISQLRADIEVTRQQMQRYNETKGIVSKE
                     DASSIPFQRLMKATSDLQNATNLRLEKLNAYNALSSANPESVTSGDPLVSQLTEEESR
                     KQREYQQGLTLFKPDYPTLVTLKTSIEKTHQSRMSAVASAYARARDAAHRDLVAAQQS
                     EDQLYAAMNAQKRETVSLNATAAGLGDLSRTLESKQSLLDQLEKRQNETEVTARLRGS
                     ASSNIHIVDHALLPTGRFNVTLQKNLQNAFPLGVVLGLAAIFFLEYMDRSIKTPEELE
                     RVTKFASLGVIPAATSADGAGYGNSGYGYGYGRGTAKLRPVVEASQEQSGIDLLPHSN
                     SRSAIAEAYRAFRTSLLLASASSPKVIVISSTFAREGKTTTSINLATVLAQMGKPVLL
                     IDADLRRPRLQKVFRGKMNLGLVNYLAASIPLDDVIQETEVPNLSLILSGPTPPNPSE
                     LLGSDRMKHLIHELRTRFAFVIFDSPPVMAVTDSIVLAANADGVILCVHGGQTPRDVV
                     QRAAERLRQSNIPVLGALLNNLDLQQYGYSFKKSYYDYYQDEPADKNAAPAKRVG"
     gene            4542..5333
                     /locus_tag="VNN25_18750"
     CDS             4542..5333
                     /locus_tag="VNN25_18750"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HXH93628.1"
                     /translation="MIDLHHHCLPGVDDGPSSMAEAVDLCKMAADEGINTIVATPHVL
                     RGRWRNRTRAEYASILDELGSKAVIAPRILLGSEYFFGHDMDEMLRNESILPLAGSRY
                     VLVEFASHAIPPLVREPLYRAQLDGWTPLIAHPERNLVFRSNPEVLASLLRIGVKTQV
                     TTGSITGDFGKEAEAAAIDWLQRGWVHVMATDAHNMVKRPPRFQAARRRVVELVGEEI
                     AQALFYENPKSIVENRGLVYDPDIPDVTEPKSEPLFTRVRKIFSR"
     gene            5330..>5802
                     /locus_tag="VNN25_18755"
     CDS             5330..>5802
                     /locus_tag="VNN25_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93629.1"
                     /translation="MKPLIRPLGALITCLVAVTASAQSFYDARFLSGVTDFNRGAYVR
                     AVDELRVAAFGRVDDLAAYEAAEIYLALASDKLDRAEDARQAALKVIQADRLNPVYVR
                     LNIPPDVRSAFERLLPAVLTREQLAHAPAFAQFAGQAPAVQSDAPRLTTRPRPPVP"
BASE COUNT         1218 a         1854 c         1637 g         1093 t
ORIGIN      
        1 cgcctttgcc gcgagcaagg agatcgccga cgatctccgc agagcacact tccaccacgg
       61 ggcgttttac ctcctgaaca cgattcagtt gtcggacatc ggctacgact cgagcttcta
      121 cgtcccgacg gccgaccgcc aatccggctt ccgcttcggc atccaggctc ctacgaggct
      181 gtacatcgtt ccgagcaaga agaccgtgta ctccatcgag gtgagaccgg aatggagttt
      241 cttcagccgc ggcagcagta aagccgtctt cggctaccag ggtcgagcag atgcccagtt
      301 cctgctcaat catctgtacc tcgactttta tgcgcagagc gcgaaccagc ttcgcgccga
      361 tgtctccgag atcgcacgcc tgctcacgga aaaggcatcg gccaccggtg tttccggcga
      421 gttgaagtac tcgagccgca ccagcatgac gtttaatgct tcggcaggaa aaagcagcca
      481 tcctctggac aagatccagc ccgacgcgcc cgtccagttg ctggaccgta gtgagcacaa
      541 ctatcgcttc accgtccacc acaagacctt ccctctgact tcgctctttg tggccacgga
      601 gttcagcgac tattcgttta ccgacgccgt gttcaagaac ggacgccgca cgtacgctgg
      661 attcggactt gtccatgaca ccggccgcac ggtgacgcag ggggaggtgg gcgagggaaa
      721 gctcgatttc attcgcccgg gccagcatga ttttcagggc gcactgggca acctgtcgag
      781 cagcaagaaa ttcggcaatt tcacgaccgg gcgcctgacg gtctcccgcg acgtcgactt
      841 ctccattttt gcgctcaata attactacat cgccgaccgg gtcagcggct tgctggcctg
      901 ggatgcaacg aggcggctga cgctcaacgc tcaggcgtcg ctcgggcgtg atctgtatga
      961 cacacccgtc atcggtccga acctcgtcct cctgaaacgg cgcgatgtgt tctccttccc
     1021 cagcgtgggt ttcctgtacg cattcgcccg tcttcggggg ggtgccgaca tcggttacgt
     1081 gcggcgcacg tcgaacttca acgtcaacct cgaccatgga atacgggttc tgtttcggtt
     1141 gtcattcacg ccatagcttg attcaaataa tcgatcggac aagagcggaa acagacgaaa
     1201 tcgtttgaca aagaattcac gcgcgacgcg cgccgaactc acgatctcgg actagcattc
     1261 gtccggtcca agctcaaatg gttcgtctac cctactgcct tcgccgccgt gcggagcggt
     1321 agatcatcgt tgggaaggaa gaaatgaaac gagttttatt ggctgcagcg gcgattggga
     1381 tgctgacgat tccggcgttt tcgcaacgtc cgccaacacc gcagccgcag attcctgccg
     1441 gctcactgct ggccgagccg ccggcaagcg ccagcgacgc gcccatcggc ccgcgcgatc
     1501 agattgaagt gaaggtctat caagacccga atctgtatac gagagcgacc gtctcggacg
     1561 acgggcggat cacgatgcca ctcatcggca aggtcgacgt ctccgggctg acaccgacgg
     1621 aagtggaaca acggatcaag gctctcctcg aagccaagta catcaacaag gccgatgtca
     1681 ccgtgacggt gacggaagcc ggcagcaagc cgatttcggt catcggcgcc gtgatgcggc
     1741 ccggccggat cggcatcacc ggaaatatca ccctgattca ggccatcaca caggcgggcg
     1801 ggctggggac cggctacggc cgcacgctct acgttctccg gacggcttcg aatggactca
     1861 cggagcagat cgccgtcgac atcgacgacc tgatggtcaa cggaaacccc gacctcaatc
     1921 tgccgttgcg cgcaaacgac gtgatcaacg tccccatcga cagctcgatc agtatctacg
     1981 tcctcggaga ggtcatgcat cccggccccg cgcagttccg ccgctcgcaa acgccgactc
     2041 ttttgcaggt gcttgcatcc gcgggcggcc cgacggaccg cgcatcgaag agagtcgttc
     2101 tgaagcgcat ggtgggagga accgagaaaa cgtttcacta tgacttccgc aagatcatcg
     2161 acggccgcca gaacgacatc gtgttgatgg acggcgaccg gctcatcttc caggagagtt
     2221 ttttctgatc gcagccgcgg gactcctccg cgggatttga aagagccgct ggaagtctct
     2281 cgatcgatga tgggttacca tcagccatgg acctgaacac tcaggaagtc catctttctc
     2341 actactggaa catcatctac aagcgatgga aagtcgcctt gtcgattctg gtcgtagtga
     2401 tgctcgggac gtacgtcgcc agttacttcg ccaagcctct ttaccgctcc acgattcaga
     2461 tccagatcga gcgggagaat ccaagtcagc tcaccgtcga tgacctgttc ggcatcgccg
     2521 catccgatca ggagtttttg cagacgcagt acgtactgct caagagccgc ggtctggccg
     2581 agaagatcat cgacgaccag aaactcctca gcgatccgtc cttttacccg gccgggatca
     2641 atggcaaaac ggccgcgcag atcgcccagg cccgcgagtc gatggtggga gcggtgatcg
     2701 catcgatcga agtcaacccc gtccgtgcga cgtcgttggt ggagatcggc tgtatcgccg
     2761 cgacaccgca gcttgcccag aagatcgcgg aaggcgtagg ggagtcctac atccggctga
     2821 acaacgagaa gaaattcgag tctgttcaaa aggccagcga ttttctcgac cgtcaaatct
     2881 cccaactgcg cgcggacatc gaagtgacac gccagcagat gcagaggtac aacgaaacga
     2941 aaggaatcgt ttcgaaggaa gacgccagca gcatcccatt ccagcggttg atgaaagcga
     3001 cttccgacct ccagaacgcc acgaatctcc gcctcgaaaa gctgaatgcc tacaacgctc
     3061 tcagcagtgc taatcctgag tccgtcacga gcggcgatcc gttggtcagc cagctcaccg
     3121 aagaagaatc gcggaaacaa cgtgagtacc agcagggtct gaccctgttc aagccggact
     3181 atccgacgtt ggtgacattg aagacctcga tcgagaagac ccatcaatcc cggatgtcgg
     3241 cggtggccag tgcctacgcc cgagcacgcg atgccgccca ccgcgatctc gtcgcggcgc
     3301 agcagagcga agaccagttg tatgcggcga tgaacgccca gaaacgcgag accgtgtcac
     3361 tcaacgccac agctgccggc ctcggcgacc tgagccgcac gctcgaatca aaacagtcgt
     3421 tgctggatca gctcgagaaa cggcagaacg agacggaagt taccgcacgc ctgcgcggct
     3481 cggcatcctc aaatattcat atcgtcgacc atgccctgct gcccaccggg cgtttcaacg
     3541 tgacgctcca gaagaatctg cagaatgcct tcccgctcgg cgtggtcctg ggtctggccg
     3601 ccatcttctt tctcgagtac atggatcgct cgatcaagac acccgaagag ctcgagcgcg
     3661 tcaccaagtt cgcctctttg ggcgtcatcc ccgccgccac ctcggcggac ggcgccggtt
     3721 acggcaatag cggttatggc tatggctacg gccgcggaac agcgaagctc cggccggtcg
     3781 tggaagcgag tcaggagcaa agcggtatcg acctgctgcc gcacagcaat tcccgctcgg
     3841 ccatcgccga agcctaccgc gcgttccgca cgtctctgct gctcgcatcg gccagctcgc
     3901 cgaaagtgat cgtcatctcc tcgaccttcg cccgtgaagg gaagacgacg acctcgatta
     3961 acctcgccac ggttctggct cagatgggca agcccgtcct cctgatcgac gccgatctac
     4021 gccgtccgcg tctgcagaag gttttccgcg gaaagatgaa cctcggactc gtgaactatc
     4081 tcgcggcaag cattcccctc gacgacgtaa tccaggaaac ggaggtcccc aacctctcgc
     4141 tcatcctctc cggacctaca ccacccaacc cctcggagct cctcggttcc gaccggatga
     4201 agcatttgat tcatgagctc cgcacccgct ttgcgttcgt gatcttcgac tcgccgccgg
     4261 ttatggccgt gaccgattcc atcgtcctgg ccgcgaacgc cgacggcgtc atcctctgtg
     4321 ttcacggcgg gcagacgccc cgcgacgtcg tgcagcgtgc ggccgagaga ctccgccaaa
     4381 gcaatatccc ggttctcggt gcgctcctca acaatctcga ccttcagcaa tacggctact
     4441 cgttcaagaa gagctactac gattactacc aggacgaacc cgctgataag aacgccgctc
     4501 cggcaaaacg cgttggataa actcggcgag gcgcgcctcg catgatcgac cttcatcacc
     4561 actgcctgcc gggcgtcgac gacggaccca gctccatggc ggaggctgtc gacctctgca
     4621 aaatggccgc ggacgaaggc atcaacacga tcgtggccac gccgcatgtg ttgcggggcc
     4681 gctggcgtaa ccggacgcgg gccgagtacg cgtcgatact cgacgagcta ggcagcaagg
     4741 ccgtcatcgc gccgcgaatc ctcctcggct ccgagtactt cttcggacac gacatggacg
     4801 agatgctgcg gaatgaatcg attcttccac tggcaggcag ccgctacgtg ctggtcgagt
     4861 tcgccagtca cgccattcct cccctggtgc gggaaccgct ctatcgcgcc cagctcgacg
     4921 gctggacccc gttgatcgct caccccgagc gaaacctcgt gtttcgatcg aatcccgagg
     4981 tccttgcctc gctgctgcgc atcggggtga aaacgcaggt aacgaccggc agcatcaccg
     5041 gggactttgg caaagaagcg gaggcggcgg ccatcgactg gcttcagcgg ggatgggttc
     5101 acgtgatggc caccgatgct cacaacatgg tcaagcgccc gccccgtttt caggccgccc
     5161 gaaggcgtgt cgtcgagctg gtaggagagg aaatcgcgca ggcgctcttt tacgaaaacc
     5221 caaaatccat cgtcgaaaat cgcggactcg tgtacgatcc tgatattcca gatgtgacgg
     5281 aaccaaaatc cgaaccttta ttcactcgcg taagaaagat tttttcaagg tgaaaccact
     5341 cattcgcccg ctcggggcgc tcatcacgtg tctggtcgcc gttacggcat ccgcgcagag
     5401 tttttacgat gcgcgattcc tgagcggcgt aacggacttc aaccgcggcg cctacgttcg
     5461 cgcggttgac gaactgcggg tggccgcgtt cggccgcgtg gatgatttgg ccgcgtatga
     5521 agccgccgag atctatctcg ccctcgcgag tgacaaactc gaccgcgcgg aagacgcccg
     5581 ccaggccgca ctaaaggtga ttcaggccga tcgactgaac ccggtctacg tcagactcaa
     5641 cattcctccc gatgtccggt ccgcgttcga aaggttgctt ccggcggtgc tcacgcgcga
     5701 acaactggct catgcgccgg ccttcgcgca gttcgcaggc caggccccgg cggtccagtc
     5761 ggacgcgccg cggctgacca cgagaccacg gccgcctgtt cc
//
LOCUS       DATLVW010000644         5255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_397635, whole genome shotgun sequence.
ACCESSION   DATLVW010000644 DATLVW010000000
VERSION     DATLVW010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397635"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VNN25_18760"
     CDS             <1..928
                     /locus_tag="VNN25_18760"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXH93630.1"
                     /translation="VSVTNMTPGKSPTAVFKITNGDGTAVDGTKLATFSPILSGPTSN
                     YGANFRENAITKGVFSATAGTTTYTFTAALPANATGTWTISADLRRNVSLKRGDGKAD
                     IAIQESTLNPIKYVAVTGTVAPRRTAVALAQCNQCHDTLELHGGQRRNIEECVICHNP
                     TASDQAMRPAAEGQPESISFQRLIHRIHTGENLTQDFTIFGFGGSKNNFNEVRFPGDT
                     RNCAKCHASTAAYTLPLQQTDIASVTTLRDYFAPQGPATAACLGCHDNKDAAAHAYLN
                     TAVFPGSTIGAEACATCHGTGKDWAVEKVHAR"
     gene            1024..1965
                     /locus_tag="VNN25_18765"
     CDS             1024..1965
                     /locus_tag="VNN25_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HXH93631.1"
                     /translation="MLLGLPALAQEKPPEKPPEPAAPVTQTTPPATSTAPAAPAAPAE
                     ATALPACSDCHADQAKAFAANAHARGQIKKGEVPNALCESCHGDGKAHIEGGGDKEKI
                     FKPVGLLGSNKTCMSCHDISTDRISRHAGMHANSAAVNCLTCHSIHSSEAHAPHLLAK
                     KQLALCDSCHMTQVSSFRNKPYAHRLGRGGMECSSCHEPHGRPGAASLRTTSAGELPC
                     LGCHADKRGPFVFQHGAIAVGDCMTCHEPHGSSNPRRLKRATVSQLCLECHSPISGTT
                     LGSQPPAFHNLSLPRYQNCTTCHVAIHGSNRDPQLLK"
     gene            1980..3920
                     /locus_tag="VNN25_18770"
     CDS             1980..3920
                     /locus_tag="VNN25_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93632.1"
                     /translation="MKRFVANWLSLPILAIAFAANAQVVPFDLEIGYRWLDLKGDTGM
                     YRTQINERDGLLIHNFTLATSDFEGHNSLTDRFRIDVSDLGSGPAQSVRMEASRANAY
                     KLRLGYRSTDAFSALPAFANPLFDQGIVPGQHTEDRRRRMFDIDLELMPDRAIAPFIG
                     FTSNDNRGPGQTTYHLGQDEFRLSQDLHERDREIRAGASFHFASIAGQLTQGWRSFRG
                     TESLSLASGAGNGNEPGTVLGQPVTATSITRTDQTHVDTPFTNFFITGQLSDRVRLIG
                     NYVRFSADSSGSENESSAGSFASFAISRFYTGLTESATSSAKNKTWRGGGRAEVNLTP
                     HIDFLAGYQREKHELNGSALINDVFLQAITFAGFDRKDIATVLQATSAIDRTDEVLSA
                     GISAHAVGPFSFRGEVRESQQQVTVAPDLSEIVVPGSQSGTFDRRIHTVDLNGAYTHS
                     GFLLGAAVKHDTADAAIFRTDYRDRNRYRVRAGWMTPKKLFRAGVTAERTNQSNDDPG
                     IGYDSKVRQYSGEIEVSPIEALHFRGSLSQFRADSNISIRFPQNFNLGESVQQEDGNA
                     REGGVSYIKGPFSIDASLSRFHNRGTLPFNIDRHKVRATWDFKNKTGLAAEMDRDKYD
                     ETSSIFSDYNATRYGLYFRWRP"
     gene            4119..4634
                     /locus_tag="VNN25_18775"
     CDS             4119..4634
                     /locus_tag="VNN25_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXH93633.1"
                     /translation="MIIMSTPDRIFPPATTSIRRSGRLYTVIALIVAAGAFIAIAMGG
                     INKNLVYYWTPTDLYANGDKAYGATIRLGGMVAPGSIHRRPGTSGVEFDVKDAARIVH
                     VTSNGVPPQMFRENIGVVVEGTLTRNGNFQSNRLMVSHNNEYRAPKGGHALSKEELQK
                     VMRSTEGMDAK"
BASE COUNT         1091 a         1869 c         1454 g          841 t
ORIGIN      
        1 cgtcagcgtc acgaacatga cgccgggcaa gtcgccgacg gccgtattca agatcacgaa
       61 cggcgacggc acggccgttg acggaaccaa gctggccact ttctcaccga tcctttccgg
      121 tccgacgtcg aactacggcg cgaacttccg cgagaacgcc atcaccaagg gcgtcttcag
      181 cgccaccgcg ggaacgacga cctacacgtt cacggccgcg cttcccgcga atgcaaccgg
      241 cacatggacg atcagcgccg accttcgccg caacgtcagc ctcaagcgcg gcgacggcaa
      301 ggccgacatc gccatccagg agtcgacgct caacccgatc aagtacgttg ccgtcaccgg
      361 cacagtcgca ccgcgccgca ccgcggtcgc gctcgcgcag tgcaatcagt gccacgacac
      421 gctcgaactg cacggcggac agcgccgcaa catcgaagag tgcgtgatct gccacaaccc
      481 caccgccagc gatcaggcga tgcgcccggc cgcagaaggt cagccggagt cgatctcctt
      541 ccagcggctg atccatcgca ttcacaccgg ggagaatctg acgcaggact tcaccatctt
      601 cggatttggc ggctcgaaga acaacttcaa tgaagtgcgc ttccccggcg acacgcgcaa
      661 ctgcgcgaag tgccacgcca gcaccgctgc gtacacgctg ccgctgcaac agaccgacat
      721 cgcatcggtt accacgcttc gcgactactt cgctccgcag ggcccggcca ctgcagcctg
      781 cctcggatgc cacgacaaca aggacgcagc cgcacacgcg tacctgaata ctgccgtgtt
      841 ccccggttcg acaatcggag ccgaggcgtg cgcgacatgc cacggcaccg gcaaggattg
      901 ggcagtcgag aaggtacacg cgcggtaagc cagcgcctgg gctcggggga gacggcatcg
      961 tctcccccgg ctctgaggaa aatagcgatg agatctcacc tgattggagc acttgcgctt
     1021 gtagtgcttc tcggcctccc ggcgctcgcg caggaaaaac ctccggagaa gccgcccgag
     1081 ccggccgccc ctgtcacaca aaccactcca cctgccacgt caacggctcc agcagcgccc
     1141 gccgcgcctg cagaagcaac cgcacttccc gcatgcagcg actgccacgc cgatcaggcc
     1201 aaagcgttcg ccgccaacgc acacgcacgc ggccagatca agaagggtga agtccccaac
     1261 gcgctctgcg agtcatgcca cggcgacggc aaagcgcata tcgaaggcgg cggcgacaaa
     1321 gaaaagatct tcaaaccggt cgggctcctc gggtcgaaca agacctgcat gagctgtcac
     1381 gacatcagca ccgaccgcat ctcccgccac gccggcatgc acgccaactc ggcggcagtc
     1441 aactgcctca cctgtcactc gattcactcg tccgaggcgc atgcaccgca cctgctggca
     1501 aagaaacagc tcgcgctctg cgactcctgc cacatgaccc aggtctcctc cttccgcaac
     1561 aagccgtacg cgcatcgcct cggcagggga gggatggagt gctcgtcctg ccacgagccg
     1621 cacggccgtc ccggcgccgc aagcttgcgt acgacatccg ccggcgagct gccgtgcctc
     1681 ggatgccacg ccgacaagcg cggtccgttc gtcttccagc acggcgcgat cgccgttggt
     1741 gactgcatga cgtgtcacga gccgcacggc tcgtccaatc cacggcgatt gaaacgcgcc
     1801 accgtgtcgc agctctgcct cgagtgtcac tcgcccatca gcgggacgac cctcggctcg
     1861 cagccgcctg cgttccacaa cctcagtctc cctcgctatc aaaactgcac cacctgccat
     1921 gttgcgatcc acggttccaa ccgcgatccg caactgctca agtaggggaa gcgatgaaca
     1981 tgaaacgatt cgtcgcgaac tggctctcac tgccgatcct cgcgattgcc ttcgcagcca
     2041 acgcccaggt cgttcccttc gatctcgaga tcggctatcg ctggctcgat ctcaagggcg
     2101 acaccggcat gtaccgcacg cagatcaatg agcgtgacgg cctcctgatc cacaacttca
     2161 ccctggcaac gtccgacttc gagggacaca actcgctgac cgaccgcttc cgcatcgacg
     2221 tcagcgacct cggctccggc cccgcgcaat cggtacgcat ggaagcgtcg cgtgccaacg
     2281 cgtacaagct ccgcctcggt taccgatcca ccgatgcgtt cagtgcgctg cccgcgttcg
     2341 ccaatcctct cttcgatcag ggcatcgttc cgggccagca caccgaagat cgccggcgac
     2401 ggatgttcga catcgatctc gagctgatgc cggaccgcgc catcgctccg ttcatcggct
     2461 tcacgagcaa cgacaaccgc gggcccggcc agacgaccta tcacctcggt caggacgagt
     2521 tccgtctctc gcaggatctg cacgagcgcg accgcgagat ccgcgccggc gccagcttcc
     2581 acttcgcatc gatcgccggt cagctcacgc agggctggcg cagcttccgc ggaacggagt
     2641 cactgtcgct cgcatcgggc gcaggcaatg gcaacgagcc gggcaccgtt ctcgggcagc
     2701 cggtcacggc cacatcgatc acgcgcaccg accagacgca cgtcgacaca ccattcacga
     2761 acttcttcat caccggacag ttgagcgatc gcgtccgcct gatcggaaac tatgtccgtt
     2821 tctctgccga ttcgagcggc agtgaaaacg aatcgtcggc aggatcattc gcatcgttcg
     2881 ccatcagccg cttctacaca gggctgacgg aaagcgcgac atcctccgcg aagaacaaga
     2941 cctggcgcgg cggtggccgt gccgaagtca atctcacgcc gcacatcgat ttccttgccg
     3001 gctatcagcg cgaaaaacac gagctcaacg gctccgcatt gatcaatgac gtcttcctgc
     3061 aggccatcac cttcgccgga ttcgaccgca aagacatcgc cacagtgctc caggcgacga
     3121 gtgcgatcga tcgtaccgat gaagtcctga gcgccggaat ctccgcgcac gcggtcggac
     3181 ccttctcctt ccgcggcgaa gtccgcgagt cgcagcagca ggtgaccgtt gctcccgatc
     3241 tttcggagat cgtcgttccg ggaagccaga gcggaacctt cgaccggcgc attcacaccg
     3301 tcgacctgaa cggcgcctac acccattccg gattcctgct cggcgcggcg gtgaagcacg
     3361 atactgccga cgcagcgatc ttccgcaccg actaccgcga ccgtaaccgc tatcgcgttc
     3421 gcgcgggttg gatgacaccg aagaaactgt tccgggcagg cgtcaccgcc gagcgcacca
     3481 atcaatccaa cgacgacccc ggcatcggct acgacagcaa ggttcgccag tacagcggcg
     3541 agatcgaagt atccccgatc gaagcactgc acttccgcgg atccctgtcg cagttccgcg
     3601 ccgacagcaa catcagcatc cgcttcccgc agaacttcaa tctcggcgaa tcggtccagc
     3661 aggaagacgg caacgcacgc gaaggcggcg tgagttacat caagggcccg ttctccatcg
     3721 acgcaagcct ctcgcgcttc cacaaccgcg gcacgctccc cttcaacatc gaccgccaca
     3781 aagtccgcgc cacctgggat ttcaagaaca agaccggcct ggccgccgaa atggaccgcg
     3841 acaaatacga cgaaacctcc agcatcttca gcgactacaa cgccacccgc tacggcctct
     3901 acttccgctg gcgcccgtag cgcagacatt cttgtctgct cccgccgggc gtccctgccc
     3961 ggcggtggtg tctgcggatg aatcggtgtt gattggcaac caaacccctg ccgggcaggg
     4021 acgcccggca gcagcagaca agaatgtctg cactacgtga ctcccatcac ccgccccggt
     4081 gaccatcacc gccgcgcatc cccagcacag gagcaatcat gatcatcatg agcacgcctg
     4141 accggatctt cccaccagcc acgaccagca tccgccgcag cggccgcctc tacacggtga
     4201 tcgccctgat cgtcgccgcc ggcgccttca tcgccatcgc catgggcggc atcaacaaga
     4261 acctcgtcta ctactggacg cccaccgacc tctacgccaa cggcgataaa gcctacggcg
     4321 ccaccatccg cctcggcggc atggtcgccc ccggctcgat ccaccgccgc cccggcacgt
     4381 caggcgtcga gttcgacgtc aaagacgccg cccgcatcgt ccacgtaacc tccaacggcg
     4441 tccccccaca aatgttccgc gaaaacatcg gcgtcgtcgt cgaaggcacc ctcacccgca
     4501 acggcaactt ccaatccaat cgcctgatgg tctcccacaa caacgaatac cgcgcaccca
     4561 agggcggcca cgcgctgagc aaggaagagc tgcagaaggt gatgcgttcg accgagggga
     4621 tggacgcgaa gtagggggcc tcagcgcacg gtcttaatgg ccatccgcag tgattctgcc
     4681 gatgaatggg cacaggtcgc gctgcgagct cggctcgcca aggtccaacc gaacctctac
     4741 gtctggtaga cgacgaggtt acctttggtg ctttgccgga agcgatttcg ggtcagcccc
     4801 tacccaccgt ggagccagac cgcagtctga tgcgatctcc accaattccg aggtagtctc
     4861 agtgcatgac gcgatcgtca ttaaaagtgg aaggccaccg cacttcggta catcgagatc
     4921 gcagcgaaac gtccgctacg gcgagttctc cgctaagatg gtgcggggaa agccgcgtga
     4981 gtattcaaac aaacctacga ctgctcgcta ccgatgtagc ggagcaaaag cggccatcgc
     5041 caaccttcgg gcggcgtctg gccgaggcat tgtcgtgggg gggaggcagg tcgggacttc
     5101 atgacttggg agacatcgtg ggctgccgag tcacccaagg aacgattgct gacgagccgg
     5161 cagccctctt ctttggccat cggcgaagtg atgtaaacga gcgactcggt gaggcggcct
     5221 tatatgccta ccatgcttca atcgagtggt gtgtc
//
LOCUS       DATLVW010000645         7333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_276939, whole genome shotgun sequence.
ACCESSION   DATLVW010000645 DATLVW010000000
VERSION     DATLVW010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276939"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VNN25_18780"
     CDS             complement(<1..501)
                     /locus_tag="VNN25_18780"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH93634.1"
                     /translation="MRVVRVVSFLLFFPVLASAAALKGVVRGLDGKPIDAATVAVEGY
                     RTAVTDPDGRFALDVPAGKYTLIVTRSGFQAMAIPASTDADVAITLRPGLAENIVVSG
                     IRAEAQTPVTKSDVDRATIERDYYGQDIPLLLRDTPSIDTYTESGVGGSGYSYITLRG
                     ISPTRIN"
     regulatory      complement(501..596)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(702..2081)
                     /locus_tag="VNN25_18785"
     CDS             complement(702..2081)
                     /locus_tag="VNN25_18785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93635.1"
                     /translation="MTDWVSAGGMILSGLIVGFMFWYGMKGRATKPDLERRDLEAKRD
                     ALLQQLRELKEMGGSEEEKRRLERETADVLRKLDVHADKLTPTKRRSDSPVRPSAEDS
                     RTGESDLRLSTADVAAATRAATMRGFLWGFASIAVLGGIAWFVTEKATPRTESMTGGG
                     APMTSSAPQASPQQPDPAMEQAEAAVAKSPDDLGMRDELAKAYLERENLMKVFEHTAY
                     VLKREPNDARALTYQALVRVSMGQSDGAVEMLQRAVKSDPDLTDAYVALAWANMQRGK
                     TSEAEGLIAEAGRRHPDQKTKLDEVLVRMKEQMKNAPPAAMPANHPALPNPDGSQNAS
                     SAMAAAQSAAPSAAPAAGGPSVHVTLTLDRSAKSSPNAVLFIIARGAGETAGPPAAVK
                     RIMAPTFPLEIDLSTADSMMGQPLPATIRIEARLDSDGDAMTKNPSDPHAIQDGVATN
                     GGKVGLVLK"
     gene            complement(2078..2578)
                     /locus_tag="VNN25_18790"
     CDS             complement(2078..2578)
                     /locus_tag="VNN25_18790"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HXH93636.1"
                     /translation="MIATFLLLAFLQIKVPDAAQFVGPPQGTPLAGAALERRTMEISA
                     ELRCPVCQGLAIGDSPSQMAANMREQVRELLSRGYTEEQILSYFEKSYGQFVLLKPKF
                     QGVNALVWLLPVLVLAIGLVIVVTKAKRLEKTPVPVAAAVAPDAASEAADDPYLAQVR
                     EMVEKS"
     gene            complement(2575..3093)
                     /locus_tag="VNN25_18795"
     CDS             complement(2575..3093)
                     /locus_tag="VNN25_18795"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXH93637.1"
                     /translation="MNRTVLIVGIVIVAAIVFVLFAGLGKDPAHIDSPLVGRPAPPFA
                     LKAVGTGETIDLETLRGKPVVINFWATWCGPCYEEHPTLVANARNLQPEVQFIGVVFN
                     DTDDKIMRFLAERGSAYPTLLDTNGKTAIAYGVGGVPETFFLNPKGTIVAKFEGPLTT
                     EALQAELAKAMR"
     gene            complement(3090..5123)
                     /locus_tag="VNN25_18800"
     CDS             complement(3090..5123)
                     /locus_tag="VNN25_18800"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXH93638.1"
                     /translation="MIPTLGRFLILASVLVATTGAFIAYAAGSKRSPEGLRWSQRFAY
                     LFAALMLAATGVMEYALITHDFSVEYVSHVGSTQVPLWVTIVSLWSSLEGSILFWGFI
                     LGIYIAATTWVNRNDHQEYMPYAIGTWLSVGAFFSFLIAGPANPFLTVANPPLDGPGP
                     NPLLQNHLLMIIHPPMLYLGYVGMTIPFGFAIAALLRGRLGQSFLRPLRASLLLPWVF
                     LTIAIMLGGWWAYEVLGWGGYWAWDPVENASLLPWLTATAALHSAIVMERRGVLKGWT
                     VTLVQASFLLTILGTFMTRSGVFNSVHSFTQSSIGPTILIFLAAVLVFSVVLLSFRID
                     SLEAEGKLDSAVSREGMFLVNNLFFVVLTFTVLVGTCFPLIVEAIKGKQMSVGRPYFD
                     AMVVPVGTALLFLIGVGPALPWGKASRKEILRALLPPLITGAIVMAIGLALGVRNVWT
                     VLTLLFGGYAAHVTMAQMWLPFRQRVKRGDAIGPALVDAQLRRGRRRFGGYIVHAGAV
                     IVFVAIAVSSTMRTQEEVTLTRGQSTTFAGYIVTYLGSEERSEPHRQSTIARFSLTKG
                     GGPPSGGGNSGGGGNIITTMEPRMNQYAAIREPIGTPAVYSTITRDYYISIMNLQGDQ
                     AGVLIINMPMVGWIWGSVIMMGIGGLVALIPSRRRTYVVSPVPAEAAGDEVPA"
     gene            complement(5347..5853)
                     /locus_tag="VNN25_18805"
     CDS             complement(5347..5853)
                     /locus_tag="VNN25_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXH93639.1"
                     /translation="MKETTNDGMPSASAALRRKSRWFMAGALVIAAAAFLVITLGGIS
                     KNLVYYWTPTDLHAAGDKAYGATIRLGGMVAPHSVQGRNGASGLEFDVKDATGIVHVK
                     SSGVPPQMFRENIGVVVEGTMTRAGYFQCNRLMVSHNNEYRAPKAGHAIDKKELQKLM
                     RSTEGLKD"
     gene            complement(5850..5990)
                     /locus_tag="VNN25_18810"
     CDS             complement(5850..5990)
                     /locus_tag="VNN25_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93640.1"
                     /translation="MTGGMIPGGWNFVVAAYSLTAVVLAIYGVSVIIRLRAEIAKESE
                     DR"
     gene            complement(5987..6691)
                     /gene="ccsA"
                     /locus_tag="VNN25_18815"
     CDS             complement(5987..6691)
                     /gene="ccsA"
                     /locus_tag="VNN25_18815"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXH93641.1"
                     /translation="MSVAASNNRFPALPWILLGLGIVCLAIGSYIGLFVAPPEALMGQ
                     VQRIMYVHVPTAWNALLAMTFAFVSALVFLFSSDWKWDARMEAAIEVGVVLAFLLCCQ
                     GSIWAKPTWGVWWDWDPRLTTTAVLLFAFLGILALRKFVEDPARRAIWSAVATIIAYA
                     DVPIVYYSVRWWNSLHQLQSSPATVSRQFWLPLRMNAFGILFLMTGFIMLRARVGSLR
                     LKHELAPPPHAAEVTA"
     gene            complement(6697..>7333)
                     /locus_tag="VNN25_18820"
     CDS             complement(6697..>7333)
                     /locus_tag="VNN25_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXH93642.1"
                     /translation="ELLLQWRSRAQFMAVFVFGAAALLLFSFAVGPVAEMLRQFSAGF
                     LWLGLLLSSTLTLAESFHAEMENRALEGLLLLPANPRALYYGKAIANWLQLMFLGVAL
                     VPVMVVLYDAGTTRILELILVIALGAAGLSAPGTLYAAMTAQVRSKQTLLPLLLFPLI
                     VPALLASVKATSLIILGDPMNQGKSWLVLLFAFDAIYWSLCGLLFHKVVEE"
BASE COUNT         1428 a         2324 c         2309 g         1272 t
ORIGIN      
        1 gttgatccgc gtcggactga tcccgcgcaa cgtgatgtac gagtaccccg atccgccaac
       61 accggattcg gtgtaggtat cgatggacgg cgtatcgcgc aagagcaggg ggatgtcctg
      121 cccgtaataa tcgcgctcga tggtcgcgcg atcgacatcg ctcttcgtca ccggcgtctg
      181 cgcctccgcg cgaatcccgc tgacgacgat gttctcggcc agtccgggcc gcagcgtgat
      241 ggcaacgtcc gcatccgtgc tcgccggaat cgccatcgcc tggaatccgc tgcgcgtaac
      301 gatgagcgtg tacttccctg ccggcacatc gagcgcgaag cggccgtccg gatcggtcac
      361 tgccgtccga taaccctcga ccgcgacggt cgcagcatcg atgggttttc catcaagacc
      421 gcgaacgaca cccttcagcg cagccgcaga agcaagaaca ggaaaaaaga ggaggaagga
      481 aacaacgcga acaaccctca tgatgactcc gatgcctttc cctacgccgg tccaaaccgg
      541 atcaggttca agggtgtgat ctcaggccgt ggttgcggcc acccctaggc ttcgctgcgg
      601 atggtactac gtgggcgtta cggtttcacg cgaaggcgcg aagccgcgaa gaaagtcgat
      661 gtggagaatt cttcgcgtct tcgcgtcttc gcgtgaaagg ttcacttcaa caccagcccc
      721 accttccccc cattcgtcgc cacgccgtcc tggatcgcgt gagggtcaga ggggttcttg
      781 gtcatggcgt cgccgtcgga gtcgaggcgg gcttcgatgc ggatggtggc ggggagcggc
      841 tggcccatca tcgagtcggc ggtgctcagg tcgatttcga gcgggaaagt gggagccatg
      901 atgcgcttga cggcggcggg gggaccagcg gtctcgccgg cgccgcgggc gatgatgaaa
      961 agcaccgcgt tgggcgaaga tttcgcggaa cgatcgagcg taagcgtcac gtggaccgag
     1021 gggccgcccg ccgcaggagc ggcgctcggc gccgcggatt gtgcggcggc catcgccgac
     1081 gacgcgtttt gcgagccgtc gggattcggg agcgcgggat gattcgctgg catcgctgcc
     1141 ggcggcgcgt tcttcatctg ctctttcatc cgcaccagga cttcatcgag cttcgttttc
     1201 tgatcgggat ggcggcggcc ggcttcggcg atcagtcctt ccgcttcgct ggtcttgccg
     1261 cgctgcatgt tggcccaggc cagcgcgacg taggcgtcgg tgagatcggg atccgacttg
     1321 acggcgcgct gcagcatctc gaccgcgcca tcgctctggc ccatcgagac gcggacgagc
     1381 gcctggtagg tcagcgcgcg agcatcgttc ggttcgcgct tgagaacgta ggccgtgtgc
     1441 tcgaagacct tcatgaggtt ctcgcgctcg aggtaggcct tggccagctc gtcgcgcatc
     1501 ccaaggtcgt cgggtgattt cgccacggct gcttcggcct gctccatcgc gggatcgggc
     1561 tgttgcggcg atgcctgcgg cgcggagctc gtcatcggcg cgccgccgcc ggtcatgctc
     1621 tccgttcgcg gcgtcgcctt ctccgtgacg aaccaggcga tgccgccgag gacggcgatc
     1681 gaggcgaagc cccagaggaa gccgcgcatc gttgcggccc gggttgcagc agcgacgtcg
     1741 gccgtagaca aacgtaggtc ggactctcca gtccgactat cttcggccga gggtcggact
     1801 ggagagtccg acctacgttt cgttggcgtc aatttgtccg cgtgcacatc cagtttgcgc
     1861 agcacgtcgg cagtctcgcg ctccaaccgc cttttctcct cttcgctgcc gcccatttcc
     1921 ttcaactcgc gaagttgttg gaggagcgcg tcgcgtttcg cttccaggtc gcggcgctcc
     1981 aggtcgggct tcgtcgcccg gcctttcatc ccgtaccaga acatgaagcc gacgatgaga
     2041 ccggacagga tcatcccgcc ggcgctgacc caatccgtca tgatttctcc accatctcgc
     2101 ggacctgcgc caggtaagga tcgtcagccg cttccgaagc cgcatccggc gcaaccgctg
     2161 ctgctaccgg cacaggcgtc ttctcgagtc tctttgcctt cgtcacgacg atcacgagtc
     2221 cgatcgccag gaccagaacc ggcagcagcc acaccagcgc gttgacgcct tgaaacttcg
     2281 gcttgagcag tacgaactgg ccatacgact tttcgaagta gctcagaatc tgctcctcgg
     2341 tgtacccacg cgagagcagc tcgcgcacct gctcgcgcat gttggccgcc atctgcgacg
     2401 gtgagtcgcc gatggccaga ccctggcaca cggggcatcg cagctcggca gaaatctcca
     2461 tcgtgcgccg ttcgagcgcg gctccggcga gcggcgtgcc ctgcggcggt ccgacgaact
     2521 gggcggcatc gggaactttg atctggagaa acgcaaggag aagaaaagtc gcgatcatcg
     2581 catcgccttt gccagctcgg cctgcagcgc ctcagtcgtc agcggtcctt cgaacttggc
     2641 cacgatcgtc cctttcggat tgaggaagaa cgtctccgga acaccgccga cgccgtacgc
     2701 gatcgcggtc ttgccattgg tatcgagcag cgtcggatag gccgagcccc gctcggcgag
     2761 gaagcgcatg atcttgtcgt cggtgtcatt gaagacgacg ccgatgaatt gcacctcggg
     2821 ttgcagattg cgcgcgttgg cgacgagtgt cggatgttct tcgtaacacg gcccgcacca
     2881 cgtcgcccag aagttgatga cgaccggctt gccgcgcagc gtctcgaggt cgatcgtttc
     2941 gccggtgcca accgccttca gcgcgaacgg cggcgccgga cgtccgacca gcggcgagtc
     3001 gatgtgcgcc ggatctttgc cgaggccggc gaagagaacg aagacgatcg cggcaacgat
     3061 gacgatgccg acgatgagga cggtgcggtt catgccggaa cctcatcccc tgcggcttcc
     3121 gccggcaccg gcgacacgac gtacgtgcgc cgccgcgaag gaatcagcgc aaccagtccg
     3181 ccgatgccca tcatgatgac ggagccccag atccatccga ccatcggcat gttgatgatc
     3241 agcacgcccg cctgatcgcc ctgcaggttc atgatcgaaa tgtagtaatc gcgggtgatc
     3301 gtcgagtaaa cagcgggcgt gccgatcggc tcgcgtatcg ccgcgtactg gttcatgcgc
     3361 ggctccatcg tggtgatgat gttgccgcct ccgcctgaat tgccgccgcc gctcggcggt
     3421 cctccaccct tcgtgagact gaaacgcgcg atcgtcgact ggcgatgggg ctcggaccgt
     3481 tcttcggagc caagatacgt aacgatgtag ccggcgaacg tcgtgctctg tccgcgcgtc
     3541 agcgtgactt cttcctgcgt gcgcatcgtg ctgctgacag cgatggcaac gaagacgatg
     3601 acggcgccgg cgtgcacgat gtatccgccg aaacggcgcc gcccacggcg gagttgcgcg
     3661 tcgacgagcg cggggccgat agcgtcaccg cgtttgactc gttgccggaa cggcagccac
     3721 atctgcgcca tcgtcacgtg tgccgcgtat ccgccgaaca gcagcgtgag cacagtccag
     3781 acgttgcgca cgccgagtgc gaggccgatg gccatcacga tcgcgccggt gatcagcggc
     3841 ggcagaagcg cgcgcagaat ctctttccgc gaggcctttc cccacgggag cgcgggaccg
     3901 acgccgatga gaaacaggag cgccgttcca accggaacca ccatggcgtc gaagtacgga
     3961 cggccgacgc tcatctgctt ccccttgatc gcctcgacga tgagcgggaa gcacgtgccg
     4021 acgagcaccg tgaatgtcag gacgacgaag aagagattgt tgacgaggaa catcccctcg
     4081 cggctcaccg cgctatcgag cttcccttcg gcttccagcg aatcgatacg gaacgagagc
     4141 agcacgactg agaagaccag aaccgctgcg aggaagatca ggatcgtcgg tccgatcgag
     4201 ctctgcgtaa acgagtgaac ggagttgaag acgcccgagc gcgtcatgaa ggtgccgaga
     4261 atggtcagga ggaaactggc ctggacgagt gtgactgtcc aacctttcaa cacgccgcgt
     4321 cgctccatca cgatcgctga atgcagcgcc gccgtcgcgg tcagccatgg caacagcgac
     4381 gcgttctcga ccggatccca ggcccagtag ccgccccatc cgagaacttc gtacgcccac
     4441 caaccgccga gcatgatggc gatcgtcaga aagacccatg gcagcagcaa cgacgcacgc
     4501 agtggccgca ggaaggattg cccaagcctg ccgcgcaaca gcgccgcaat cgcgaaaccg
     4561 aacggaatgg tcatgccgac gtagccgagg tagagcatcg gcgggtggat gatcattagt
     4621 agatggttct gcagtagcgg attcgggccc gggccgtcga gcggcgggtt ggccaccgtc
     4681 aggaacggat tggctggtcc ggcgatcaga aagctgaaga acgcgccgac cgagagccag
     4741 gtgccgatcg cgtacggcat gtactcctga tgatcgttgc ggttcaccca agtcgtcgcg
     4801 gcgatgtaga tgccgaggat gaagccccag aaaaggatgg agccttcaag cgaggaccag
     4861 aggctgacga tcgtcaccca gagcggcacc tgcgtcgagc cgacgtgcga gacgtactcg
     4921 acactgaaat cgtgcgtgat gagggcgtac tccatcacgc cggtcgccgc cagcatcagc
     4981 gccgcgaaca aatacgcgaa gcgctgcgac cagcgcagcc cttccggtga acgcttgctg
     5041 ccggcggcgt aggcgatgaa tgccccggtc gtcgcgacga gcacggaagc cagaatgagg
     5101 aatcgtccga gtgttggaat catgttgtcg tcagttggcc ctagccggtg accattggtc
     5161 ggtgtcagtc atcacatcag tcatgtgcgt tggtacgtaa tcttcgctcg tcgttctgag
     5221 actgtcggag ctagctcctc cgcctgtcac tggcgaagga cctggatgcg acgtacgagg
     5281 catgcgtcgt cattaagatt cttcgccccc cttcggcagc tcaggatgac gttatcttcg
     5341 gaagacctaa tccttcaaac cttccgtact ccgcatcaac ttctgcaatt cctttttgtc
     5401 gatggcgtgc cccgctttcg gggcgcggta ttcgttgttg tgcgagacca tcagccggtt
     5461 gcactgaaag tagccggcgc gcgtcatcgt cccttcgacc acgacaccga tgttctcccg
     5521 gaacatctgc gggggaacgc cggacgactt cacgtgcacg attccggtgg cgtccttgac
     5581 gtcgaattcc agtccggagg caccgttgcg gccctgcacg gagtgcggcg ccaccatccc
     5641 gccgagccgg atcgtcgcac cgtaggcctt gtcgcccgca gcgtgcaggt cggtcggcgt
     5701 ccagtagtag acgaggttct tgctgatccc gccgagcgtg atcacgagaa atgctgccgc
     5761 ggcaatgacg agcgctccgg ccatgaacca gcgcgatttg cgccgcaggg cagcgctcgc
     5821 cgacggcatc ccgtcgttgg tcgtttcctt catcgatcct ccgattcttt cgcgatctcg
     5881 gcgcgcaacc ggatgatcac cgatacgccg taaatcgcca gcacgaccgc cgtcagtgaa
     5941 tacgcggcga cgacgaagtt ccatccgccg ggaatcatcc cgcccgtcat gccgtcacct
     6001 cggcagcatg cggcggcggc gcgagctcat gtttcagccg cagcgatccg acgcgcgccc
     6061 gcaacatgat gaatcccgtc atcagaaaga ggataccgaa cgcgttcatt cgcagcggca
     6121 gccagaactg acgcgagacc gtggccgggc tcgactgcag ttgatgcaac gagttccacc
     6181 agcgcactga gtaataaacg atgggaacat cggcgtacgc gatgatcgtg gcgaccgccg
     6241 accagatagc gcgccgcgcc ggatcttcga cgaacttccg aagcgccagg attccgagaa
     6301 acgcgaagag caggacggca gtcgtcgtca accgcggatc ccaatcccac cagacgcccc
     6361 aggtcggttt ggcccagatc gagccctggc agcagagaag aaacgcgagg acgacgccga
     6421 cttcgatggc cgcctccatc cgcgcgtccc atttccagtc cgagctgaag aggaagacga
     6481 gcgcgctcac gaacgcaaag gtcatcgcca gcagggcgtt ccaggcagtc ggcacatgca
     6541 cgtacatgat ccgctgcacc tgccccatca acgcctcggg aggcgcgacg aaaaggccga
     6601 tgtaggagcc gatcgccagg cagacgatcc cgagccccag cagaatccag ggcaacgccg
     6661 gaaaccggtt gttactcgca gcaacgctca tagacctcac tcttcgacga ccttatggaa
     6721 aagaagtcca caaagagacc aataaattgc atcaaaagcg aagagaagca caagccatga
     6781 tttcccctga ttcatcgggt cgccgagaat gatcagcgaa gtggccttga ccgacgcgag
     6841 aagtgccgga acgatcaacg gaaacaggag cagtggcagc agcgtctgct tcgagcgcac
     6901 ctgcgccgtc atcgccgcgt aaagagtgcc cggagccgac aatcccgccg cgccgagtgc
     6961 gatgacgagg atgagctcga ggatgcgcgt cgtcccggcg tcgtagagca caaccatcac
     7021 cggaacgagg gcgacgccga gaaacatgag ctgcagccag ttggcgatgg ccttgccgta
     7081 gtagagcgca cgcggattgg ccggcaggag cagcaggcct tccagcgccc ggttctccat
     7141 ctcggcgtgg aaactctcgg cgagggtgag cgtcgaggag aggagcagtc ccagccagag
     7201 gaacccggcc gagaattgcc ggagcatttc cgctaccggc ccgacggcga agctgaagag
     7261 cagcaacgcc gcggcaccga agacgaacac cgccatgaac tgggcgcggc tgcgccattg
     7321 gaggagcagc tcc
//
LOCUS       DATLVW010000646         6886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_361898, whole genome shotgun sequence.
ACCESSION   DATLVW010000646 DATLVW010000000
VERSION     DATLVW010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361898"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1082
                     /gene="hemC"
                     /locus_tag="VNN25_18825"
     CDS             171..1082
                     /gene="hemC"
                     /locus_tag="VNN25_18825"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXH93643.1"
                     /translation="MTRLVRIGTRGSQLALWQANEVANKLSEHGYRTEIVVFKTTADK
                     RQDVPLASIGGKGLWVKELEEALLRGAIDIAVHSLKDVPSIIPDEFLLAGFLERADPR
                     DAWVNIEGKPIAALPDGAVIGTSAPRRRSQIRTLYPRLRIDDIRGNVDTRINKLRAGL
                     YDGAILASAGLTRLGRASEITSYFSTEEMLPACGQGIIAIETLREGSTRDIAASIDHA
                     PTAVSAQCERGVLQKFGTLLDCYSACAVHATQEDHSITIRAFFGEIDGDRAIRVTREG
                     NDADAVVDDVYRALVAEGAMELVGALG"
     gene            2155..3630
                     /gene="cobA"
                     /locus_tag="VNN25_18830"
     CDS             2155..3630
                     /gene="cobA"
                     /locus_tag="VNN25_18830"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXH93644.1"
                     /translation="MTGKVFLVGAGPGDPALLTLRAAELIASADFIAIDALVSQEIAN
                     RVSQTAEVVYVGKRAAAHTLPQDQINQLLIDEAKKGKKVVRLKGGDPFVFGRGGEEAE
                     ELVAAGVAFEIVPGISSSIAGPAYAGIPVTHRSFATSLTLVTGHESESSTGIKWDALA
                     QLDGTIVFMMGFGNLGTIVSKLIEHGVTPDRPIAVISKATTPEQRTVAGTLATIEAKV
                     VEAKLPTPALIVVGDVVKMHDAINWFETKPLFGKRIVVTRAREQASDLKRLFQESGAT
                     VIEFPTITIAPPESFESLDRCIDERFDWLVFTSSNGVAAFFERLFEKGRDVRALAGTR
                     VACVGETTAAALRVRGLAPDVVPEKFMSSALIPLFDDDQKGVRIAVVRAAEGREEFVE
                     EMRRRGAEVHLAVAYRTMPVAANANELHDIDVVTFTSASTVDNFFASIGDHSVNGALL
                     ASIGPMTSEAIRKQGRDVGVEAAIASVQGLHDALVARLIVA"
     gene            complement(3667..4695)
                     /locus_tag="VNN25_18835"
     CDS             complement(3667..4695)
                     /locus_tag="VNN25_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93645.1"
                     /translation="MTRTRGLSLAAIITLLLLPLALEARNDPRCESFEARLKKTYGFR
                     PSQLDASSQDAKAKAMDAIWTAVKEDPATLVPCLKSALAQPTQDGWFLYDGNQLLLSV
                     APSREVKQALLDALGRVSLDDVDLRSWVATTSALGTEGFDTSALGRRWLAYPKAAYSL
                     PEHGGYIVDRENGAMFIFGAEDETFATPALTEICRTSTGEVKEIAAWLLMSQATRSAL
                     QAVATLNTDGLSPKAIASRAALLRAPDLIIPRNPPRTTRKEFLEAFSALLAGDQAPFD
                     HLVQAVPDGERDLVAVVNDTDLDTIRRVRRWFIARNNQHAIEYYNQFCQILMTLVWKP
                     EVFHKDVK"
     gene            complement(4743..5327)
                     /locus_tag="VNN25_18840"
     CDS             complement(4743..5327)
                     /locus_tag="VNN25_18840"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH93646.1"
                     /translation="MIIECTSCQARYQYDEDRFERKPAKKIKCAKCGTVFEIHNPAFA
                     PKPSSPVGELTALRREETRTRSTEMPETTAEAPLPKRDTGPKPILTLLPPGRRISIAI
                     LAGPDAGTMYRLEKPRTTIGRSGADLTINDTEASRQHAAIVVREDSITVEDLKSTNGT
                     MVNGEKLTRPAELQDKSEFQIGTTSLMLIVTEEG"
     gene            5407..>6886
                     /locus_tag="VNN25_18845"
     CDS             5407..>6886
                     /locus_tag="VNN25_18845"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HXH93647.1"
                     /translation="MKRLILLFAAVAAASCQPKPATTESSASTPAAATSSIAADTPQR
                     LAQLPRTVIDYDRSLLDDNERQVVAKLIEASKQIDEIYWRQVSENNPALRDKLTKQAG
                     NSALDRAGLDYFIANKGPWDRLKGDEPFIGTTKKPAGASFYPADMTKEELEKYVAAHP
                     DKKDQLEGLFTVVRREGANLVGIPYGTYYKDALDQAATHLREAANISNDGALKRFLVL
                     RADAFKSDNYRDSDMAWMDLTGNIEVVIGPYEVYEDNLFNYKASYESFVTVVDKPESA
                     KLAAYAKALPDMERNLPEPEQYKNPNRGSESPIKVVQELYTAGDARRGVQTAAFNLPN
                     DEVVREKKGSKKVLLKNVMDAKFRQSGKPISARVLDPSLQSLVSFDAYFNHTLFHELS
                     HGLGPGFLTQPDGKRVEVRIPLKELFSTVEECKADVLGVWNLLYAQQHGLVTAFNERQ
                     LLATYAGLMFRSTRFGVGEAHGRGTAIQWNWLREHGAVTPIAG"
BASE COUNT         1349 a         2136 c         2105 g         1296 t
ORIGIN      
        1 ctccgcaaga ttggcccgat gagccccgag cagcgcgccg ccatcgagca gctctccaca
       61 cagatggtga acaagatcct ccactacccg atcctgcagc ttaaagaagc tgaggagccg
      121 gccgagcgcg agaccttgcg caagacgatc cggaagattt ttggattgcg atgactcgcc
      181 ttgttcgcat cggcacccgc ggcagccagc tcgcactctg gcaggcaaat gaagttgcaa
      241 acaagctgtc agagcatggc taccgcacgg aaatcgtcgt gttcaaaacg accgccgaca
      301 aaaggcagga cgtgccgctg gcatcaatcg gggggaaggg attgtgggtc aaggagctcg
      361 aagaagcgct tctgcgcggc gcgatcgaca tcgccgtcca tagcctgaaa gacgttccgt
      421 cgatcattcc cgatgagttc ctcctggccg ggttcctcga acgtgccgat ccgcgcgacg
      481 cgtgggtgaa catcgaaggg aagccaatcg ctgctttgcc ggatggtgcg gtgatcggaa
      541 cgagcgctcc gcgccggcgc tcacagattc gcacgctcta tccccgcctg cgcatcgacg
      601 acatccgcgg caacgtcgac acgcgcatta acaagctgcg tgcgggcctc tacgatggcg
      661 ccatcctcgc cagcgccggc ctcacccgcc tcgggcgcgc aagcgagatc acgtcctact
      721 tttcgaccga ggagatgctc cccgcctgcg gccagggaat catcgccatc gagacgctgc
      781 gcgagggctc aacgcgggac atcgctgcat cgatcgatca cgcgccaacc gcagtgtccg
      841 cgcaatgcga acgcggcgtg ctacagaagt tcggcaccct gctcgactgc tattccgcct
      901 gcgccgtcca cgcaacacag gaagaccact cgatcacaat ccgcgccttc ttcggcgaga
      961 tcgacggcga ccgcgccatc cgggtaaccc gagagggcaa tgatgcggat gcggtcgtcg
     1021 atgatgtcta tcgcgcgctg gtagccgaag gcgcaatgga gttggtgggg gcattgggat
     1081 gaacgcgacc aaacgcacct tccccctgca cagcagactt caatcgagcg ccttcgcccc
     1141 cgcgcttttc agcgggggag aaggtggccg aaggccggat gagggggcac gcgatagagc
     1201 cgtgcaagtc cgaaacaacc tcagtttctc gctcgattgc tcgtcttcgt cctattcacg
     1261 tcccccctca tccgccttcg gcaccttctc cccccgcaaa aagcgcgggg ggagaaggcg
     1321 ctcgattgga ggtttccgcc aaagcctcaa gttagagcgt gatcacgctc cggagtttcg
     1381 ttcatgaccg cgggcgttgg gatgagtgcg aaaggcccag cggaacgccg gcgtcccgcc
     1441 ggctggccag ttgccgtccc ggcgcctcgg gcatgcgctc atacgctaac tgaaatcatt
     1501 agcattgaac ttgatagcag cgcacttcaa tcgagcgcct tcgcccccgc gcttttcagc
     1561 gggggagaag gtggccgaag gccggatgag ggggcacacc acagaacggt gctggtccca
     1621 aacaacctca gtttctcgcg cgattgctcg tcttcgtcct attcaactcc cccctcatcc
     1681 gccttcggca ccttctcccc ccgcaaaaag cgcggggggc gaaggcgctc gattggaggt
     1741 ttccgcgaga gttgcaagat agagcgtgat cacgctccgg agtttcgttc atgaccgggg
     1801 gcgttggtat gagcgcgaaa agcccaggcg ccgtcccggc gcctcgggaa tgcgctgaaa
     1861 tcgctagcaa tgaacttgat agcagcacac gtcaatcgag ccccttcgcc cccgcgcttt
     1921 tcagcggggg agaaggtggc cgaaggccgg atgagggggc acaccacaga acggtgctgg
     1981 tcctcaacaa cctcactccc gcgctcgatt gctcgtcgtc gttttattca agtcccccct
     2041 catccgcctt cggcaccttc tccccccgca aaaagcgcgg ggggcgaagg cgctcgattg
     2101 gaggtttccg cgagagattg aagaaagaat tgagtaacgt gaggagtcgc acccatgacc
     2161 ggtaaagtct tcctcgttgg cgccggtcct ggtgatcccg cgcttctcac gctccgcgcc
     2221 gcggagctga ttgcgtccgc tgacttcatc gccatcgatg ctctcgtgtc gcaggagatt
     2281 gcaaaccgcg tttcgcagac cgccgaggtc gtatacgtcg gaaagcgcgc cgcggcgcat
     2341 acgctgccgc aggatcagat caatcagctc ctcatcgatg aagcgaagaa gggaaagaag
     2401 gtcgtccggc tcaaaggtgg cgatccgttt gtcttcgggc gcggcggtga ggaagccgaa
     2461 gagttggtcg cggcgggcgt ggcattcgag atcgtgccgg ggatttcgtc gtcgatcgcc
     2521 ggacctgcct acgccggcat cccggtgacg caccgttctt tcgcgacgtc gctcacgctc
     2581 gtcactggtc atgaatccga gagctcgacc ggcatcaagt gggatgcgct cgctcaactc
     2641 gacggcacca tcgttttcat gatgggtttt ggcaacctcg gcacgatcgt ttccaaactg
     2701 atcgagcacg gcgtgacgcc cgatcgcccc atcgccgtga tctcgaaggc gaccacaccc
     2761 gaacagcgca ccgtcgctgg aacgctggcg acaatcgaag cgaaggtggt cgaagcgaaa
     2821 ctccccactc cagcattgat cgtcgtcggc gacgtcgtaa agatgcacga tgcgatcaac
     2881 tggttcgaaa cgaagccgtt gttcggcaag cggatcgttg tcacgcgcgc ccgcgagcag
     2941 gcctcggatc tcaagcggct gttccaggag tcgggcgcaa ctgtcatcga gtttccgacg
     3001 atcaccatcg cgccgccgga gtcgttcgaa tcactcgatc gctgcatcga cgagcgcttc
     3061 gactggctcg tcttcacatc gtcgaacgga gttgccgcct tcttcgagcg tctcttcgag
     3121 aaaggtcgcg acgttcgcgc gctcgcgggc acgcgcgtgg cctgcgtcgg cgagacgacc
     3181 gcagccgccc ttcgcgtgcg cggcctcgca cccgacgtcg taccggaaaa gttcatgtcg
     3241 tccgcgctaa tcccactgtt tgacgacgat cagaaaggcg tgcgcattgc cgttgttcgc
     3301 gctgccgagg ggcgcgagga gtttgttgag gagatgaggc gccgcggcgc tgaggtacat
     3361 cttgccgtcg catatcgaac gatgccggtg gctgcgaatg ccaacgagct gcatgacatc
     3421 gatgtcgtca cgttcacgag cgcttcgacg gtcgacaatt tcttcgcttc gattggcgat
     3481 cactctgtca acggcgcgtt gctggcttcg atcgggccga tgacgagtga ggcgattcga
     3541 aagcaggggc gtgacgtggg cgttgaggct gctatcgcaa gtgttcaggg actgcacgat
     3601 gccctggtcg ctcgcctgat tgtggcgtaa aactgaacgc cgttttcgaa ggcggcctgg
     3661 atgggcctac ttcacgtcct tgtggaacac ttcgggcttc cagaccagcg tcatgaggat
     3721 ctggcagaac tggttgtagt actcgatggc gtgctgatta ttcctcgcga tgaaccaacg
     3781 ccgtaccctt cgaatcgtgt cgagatccgt gtcattcaca accgcgacca ggtctcgttc
     3841 gccgtccggg acggcctgga cgagatgatc gaacggcgct tgatccccgg cgaggagcgc
     3901 cgagaaggcc tccagaaact ctttgcgtgt cgtgcgaggc ggatttctgg ggatgatgag
     3961 atctggagct cgaagcaacg cggcgcggct tgcaatggct ttgggcgaca gcccgtcggt
     4021 gttgagcgtt gccaccgctt gtagcgcgct gcgtgtcgcc tgactcatca ggagccaagc
     4081 cgcaatctcc ttgacctcgc cggtggatgt ccggcaaatc tccgtgagcg caggtgtcgc
     4141 gaacgtctca tcctcagcgc cgaagatgaa cattgctccg ttctcgcgat cgacgatgta
     4201 gccgccgtgt tccggcagtg agtatgcggc cttcgggtat gccagccagc ggcggcccag
     4261 cgcggaggta tcgaagccct ctgtgccgag ggctgaggtg gttgcaaccc atgaacgaag
     4321 atcgacgtcg tccagagaaa cgcgcccaag agcatcgagc agtgcctgct tcacttcccg
     4381 ggaaggagcc accgacaaga gcaactgatt gccgtcgtaa agaaaccaac catcttgcgt
     4441 cggctgagcg agcgcgctct tgagacaagg cacgagcgtg gccggatcct ccttgacggc
     4501 agtccagatc gcatccattg ccttcgcttt cgcgtcctgg gaagaagcgt cgagttgaga
     4561 agggcgaaag ccgtaggtct tcttgaggcg ggcctcgaag ctctcacagc gagggtcatt
     4621 ccgtgcctcg agcgcaaggg gtagaagcag aagggtgatg atggcggcca gcgacagccc
     4681 tcgagtccgt gtcatttacg ctccattggt gaggtaggaa gtttccgatc gattccgagt
     4741 cgttaccctt cctcagtcac gatcagcatc agcgacgtgg tcccgatctg aaactccgac
     4801 ttgtcctgaa gctcggcggg ccgggtgagt ttctcgccgt tgaccatcgt gccgttcgtc
     4861 gatttgagat cttcgacggt gatgctgtct tcccgcacca cgatcgcggc gtgttgacgc
     4921 gaggcttccg tgtcgttgat agtcaggtcg gcgccggatc ggccgatagt agtgcgcggc
     4981 ttttcgaggc ggtacatcgt tcccgcgtcg gggccggcca ggatagcgat cgagatgcgc
     5041 cggccaggcg gaagtagcgt gaggatcggc ttcgggccgg tatcgcgctt gggcaacggt
     5101 gcctccgccg tcgtctccgg catctccgtg gagcgggtgc gggtctcttc acgccgcaag
     5161 gcagttaatt cgccgacggg tgatgatggc ttcggcgcaa acgcgggatt gtggatctcg
     5221 aagacggtcc cgcatttggc gcacttgatc ttcttcgccg gtttccgctc gaagcggtcc
     5281 tcgtcgtact ggtaccgcgc ctgacagctc gtgcactcga tgatcaagag aaacctcggg
     5341 aaacgtggcc cgacgatagc agagcgctcg gctcacatac aatcctgcct cccgaaggag
     5401 aacgcgatga aacgactgat cctcctcttt gccgccgttg cggccgcctc ctgccagccg
     5461 aagccggcaa ccacggagtc atctgcttcc acaccggctg ctgcgacctc gagtatcgcc
     5521 gccgacaccc cgcagcgtct ggcgcagctt ccgcgcacgg tgatcgacta cgaccgatcg
     5581 ctgctcgacg acaacgagcg tcaggtcgtc gctaagctca tcgaggcgtc aaagcagatc
     5641 gacgagatct attggcggca ggtgtccgaa aacaaccctg cgcttcgcga caagctcacc
     5701 aagcaggctg gcaattcggc gctcgatcgc gcgggacttg actacttcat cgccaacaaa
     5761 ggtccctggg accggctgaa aggcgacgag ccgttcatcg ggacgacgaa gaagccggcc
     5821 ggcgcttctt tctatccggc cgacatgacc aaggaagagc tggagaagta tgtagccgct
     5881 catcccgaca agaaagatca gttggaaggg cttttcaccg tcgtccgccg cgagggcgcg
     5941 aacctggtgg gcatcccgta tgggacgtac tacaaagacg cactcgatca ggccgccaca
     6001 catcttcgcg aggccgccaa catttcgaac gacggcgctc tgaagcgctt tctggtactg
     6061 cgcgcggacg cattcaagtc cgacaactac cgcgatagcg acatggcctg gatggatctg
     6121 accggcaaca tcgaagtggt catcggtccg tacgaggtct acgaagacaa tctcttcaac
     6181 tacaaggctt cctacgaatc gttcgtcacc gtcgtcgaca agccggaaag cgcgaagctc
     6241 gccgcatacg cgaaagcgct gccggacatg gagcgcaatc ttcccgagcc cgagcagtac
     6301 aagaatccga accgcggcag cgaatcgccg atcaaagtcg tgcaggagct ttacaccgcc
     6361 ggcgacgcgc ggcgcggggt acagaccgcg gcgttcaatc tgccgaacga cgaagtggtg
     6421 cgcgagaaaa agggctcgaa gaaagtcctg ctcaagaacg tgatggacgc caagttccgt
     6481 cagagcggaa agccgatctc tgcccgcgtg ctcgatccgt cgctgcaaag cctcgtttcg
     6541 ttcgacgcct atttcaatca caccctcttc cacgagctct cccacggtct cgggcccggt
     6601 ttcctcacgc agccggatgg aaagcgcgtc gaagtgcgca tcccgctcaa agagcttttc
     6661 tccaccgtcg aagagtgcaa agctgacgtc ctcggcgtct ggaacctcct ctacgcgcag
     6721 caacacggcc tcgtaacggc gttcaacgag cgtcaactgc tggcgaccta cgcgggtctg
     6781 atgttccgct ccacgcgatt cggcgtcggc gaagcgcacg ggcgcggaac ggccatccag
     6841 tggaactggc tgcgcgagca tggcgccgtc acaccgatcg ccggcg
//
LOCUS       DATLVW010000647         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_196790, whole genome shotgun sequence.
ACCESSION   DATLVW010000647 DATLVW010000000
VERSION     DATLVW010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196790"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VNN25_18850"
     CDS             <1..1021
                     /locus_tag="VNN25_18850"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH93648.1"
                     /translation="WLYTDGVQSQYSQSGPPVYWNPYLSVGTHILILTYSGNATQPLG
                     TSSPLTVVVHKARSITTVENPPYLVQYGNQPVVRVSLQGDPFGGVPGGNVSLSEGSTV
                     LTTIFADKRCCSGGITVEMTLPVLTPGVHYIRATYVGDESYLPSQSASFQLRVLPAGT
                     FVLDAEAGADWIQARGFFNAPPNTSYLVHRRVGSGPWTTSVQGSPYYSEFKPQPGTPY
                     TYWIEALDQASHVIATSNADMAMIVAFTDDPITITTPVKALHEAEIVAAANSLRTLAG
                     LPAFPAPGNAGDVIHASHILAIRNAINEARVALGAAPATFSTGVAAGSRVRVQDIQDL
                     REAMR"
     gene            1097..2587
                     /locus_tag="VNN25_18855"
     CDS             1097..2587
                     /locus_tag="VNN25_18855"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HXH93649.1"
                     /translation="MATKPKAHARKAVKRLPRRVRDIDTPASTLSANQIAQAYVRSIT
                     PQLALGESTLHFDRVKTSILGSHVLYQQYRGELPISGAWLRIDVSPDGRVFNVLNDLI
                     PEPAKLRATKAKTIGARVADKRAREAVNAGTVRVVEHERVCYPVNGVPRSCWKVVVRT
                     VKPRGAWKVYVDASSGSVVARFDLAKHVVGHGRVFDPNPISSLNDPTLTPTSVIPQQA
                     YHDVDLNGLDGSGFLDGEFVSTRPTQDRTSSAANTFVFTRDDRAFREVMAYFHIDRVR
                     RYLGDLGFGDVLNRAVEVNIDATQDEGSQYDPITKSILFASGGVPDAEDGEIIVHEYG
                     HAIQDDQVPGFGQGAESRAMGEGFGDFLSASFFADAKSPEMRPMVGTWDAAGYPKPKP
                     FLRRVDSTKKYPKDIKNNIYSDSAIWSASLWQMRERLGRATAEKLVIAHHHLLSPAAT
                     FEDAANALLKADQELNGGANAAAVKKIFTDRGILPRAKKKASGGLK"
     gene            2584..>3179
                     /locus_tag="VNN25_18860"
     CDS             2584..>3179
                     /locus_tag="VNN25_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93650.1"
                     /translation="MNARSKILMLVVFLATVAIAVPAFAQTVTGATEVPSKHDQTTQT
                     FGSQPIDLPRGKGFFAEHLVKPLPLPPPAPKGKATPTVNVVQSTATKTLGVSTPVHFP
                     GISDGSTYSVDAAPSDTTGAIGSTQFVQWVNEAVQVFNRDGSGAYGPTLGRALWKDFG
                     GDCQNLNDGDPIVQYDQLADRWILTQFAVSSTPFVQCVA"
BASE COUNT          617 a          998 c          973 g          591 t
ORIGIN      
        1 ctggctctac accgacgggg tacagtcgca atactcacaa agcggtccgc ccgtctactg
       61 gaatccgtac ctttccgtcg gtactcatat ccttattctc acgtattccg ggaacgcgac
      121 acagccactc ggcacgtcga gccctctaac ggtggtcgtg cacaaggcgc gatcgatcac
      181 cacggtggag aatccgccgt acctcgtcca gtacggcaat caaccggttg tccgcgtgtc
      241 acttcaggga gacccgtttg gcggtgtgcc gggcgggaac gtcagtctct cggaaggatc
      301 aacggttctg accaccatct ttgccgataa gcgctgctgc tccggcggga tcaccgtcga
      361 gatgaccttg cctgtcctga ctcccggcgt tcactacatt cgcgcgacgt acgtcggtga
      421 cgaaagctac ctgccttcgc agtcagcgtc tttccagctg cgggtgttgc cggcaggaac
      481 gttcgttctg gatgctgagg ccggcgctga ttggatccag gccagaggtt tcttcaacgc
      541 gccaccgaac acgagctatc tcgtccatcg tcgtgtcggc tccggcccct ggacgacctc
      601 agtgcaggga agtccctact attcggagtt caaacctcaa ccggggacgc catacactta
      661 ctggatcgag gcgctcgacc aggccagcca tgtcatcgca accagcaatg ccgacatggc
      721 gatgatcgtt gccttcacgg acgatccgat cacgataacg acgcccgtca aggcattgca
      781 tgaggccgag attgtcgctg ccgcgaatag tctccgcact ctggccgggc ttccggcatt
      841 ccctgccccg ggaaacgcgg gcgatgtcat ccacgcatcg cacattctgg ctatccgaaa
      901 cgcgatcaat gaagcgcgcg tagctctcgg agcagcgcca gcaacgttct ctaccggtgt
      961 cgccgccggt tcgcgggtcc gcgtccagga tattcaggat cttcgcgaag caatgcgatg
     1021 agcgaggttt caggctaccg ttgtgcgcct gtttcgcggg tagggaaaag ctgccctcac
     1081 gcctctcctt attagcatgg cgacaaaacc gaaagcccac gcccggaagg cggtcaagcg
     1141 gttaccgcgg cgtgttcgcg acatcgacac gccggcaagc acactgagtg cgaaccagat
     1201 tgcgcaggca tacgtccggt cgatcacgcc gcagttggcg ctcggggaga gcacgcttca
     1261 tttcgaccgg gtgaagacgt cgatcctcgg cagtcacgtt ctctatcagc aatatcgcgg
     1321 cgagttgccg atctccggcg cgtggctgcg cattgatgtc tcgcctgacg gacgcgtttt
     1381 caatgtgctc aacgacctca ttccggagcc tgccaagctt cgagcgacga aagccaagac
     1441 gattggtgcg agggtggcgg acaagcgggc tcgcgaggcg gtcaacgcgg gaacggtgcg
     1501 cgtcgtggag cacgagcgcg tctgctaccc ggtcaacgga gttccgaggt cgtgctggaa
     1561 ggtcgtcgtg cgaacggtga agccgagggg cgcgtggaag gtgtatgtag acgcgagcag
     1621 cggctcggtg gtggcacggt tcgatctcgc gaagcacgtc gtcgggcatg gaagggtctt
     1681 cgatccgaat cccatcagtt ccctcaacga cccgacgctg accccgacgt ccgtgattcc
     1741 gcagcaggcg tatcacgacg tcgatctgaa cggattggat ggcagcggct ttctggacgg
     1801 ggagttcgtc agcacgcgcc cgacgcagga ccggacgagc agcgcggcga acaccttcgt
     1861 cttcacgcgc gacgaccgcg ccttccgtga ggtgatggcc tatttccaca tcgatcgcgt
     1921 tcgccgctac ctcggcgacc tcggatttgg cgacgttttg aatcgcgccg ttgaggtcaa
     1981 catcgacgcc acccaggatg aaggatcgca atacgatccg atcacgaagt cgatcctgtt
     2041 cgcatcgggc ggcgtgcccg acgccgagga tggggagatc atcgtgcatg aatacggaca
     2101 cgcgattcag gacgatcagg ttcccggatt cggtcagggg gcggagtcgc gggccatggg
     2161 cgaaggcttc ggcgactttc tctccgccag cttctttgcc gacgcgaagt caccggagat
     2221 gcgtccgatg gtgggcacgt gggatgccgc cggctatccg aagccgaagc cgtttctccg
     2281 ccgcgtggac agcacgaaga agtatccgaa agacatcaag aacaacatct actctgacag
     2341 cgcgatctgg tccgcgtccc tctggcagat gcgcgaacga ttggggcgcg cgacggcgga
     2401 gaaactcgtc atcgcccatc accatctgct ctcccccgcc gccacgttcg aagacgccgc
     2461 aaacgcgctg ctcaaggccg accaggagct caacggcggc gcgaatgcgg ctgccgtgaa
     2521 aaagattttt accgaccgcg gcatcctgcc gcgagcgaaa aagaaagcga gtggaggact
     2581 caaatgaacg cccgatcaaa gatcctcatg ctggttgttt tccttgccac cgtcgcgatc
     2641 gcagtgccgg cgtttgccca gacagtgacc ggcgccaccg aagtgccttc gaagcacgat
     2701 cagacgacgc agaccttcgg ctcgcagccg atcgacttgc cgcggggaaa gggcttcttc
     2761 gcggaacatc tcgtcaagcc gcttccgctg ccgccgcccg cgccaaaggg gaaggccacc
     2821 ccgacggtga acgtcgtcca gtcgaccgcc accaagacgc tgggggtatc gacgcccgtg
     2881 catttcccgg gaatcagcga tggcagcacc tattcggtcg atgccgctcc gtccgatacc
     2941 accggcgcga tcggttcgac ccaattcgtg cagtgggtca acgaggcggt gcaggtcttc
     3001 aaccgcgacg gaagtggcgc ctacggtccg acactcggcc gtgcgctgtg gaaggacttc
     3061 ggaggcgatt gtcagaacct gaacgacggc gatccgatcg tccagtacga ccagctggct
     3121 gaccgctgga tcctgacgca gtttgccgtc tcctcgacgc ccttcgtgca gtgcgtggc
//
LOCUS       DATLVW010000648         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_397721, whole genome shotgun sequence.
ACCESSION   DATLVW010000648 DATLVW010000000
VERSION     DATLVW010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397721"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="eno"
                     /locus_tag="VNN25_18865"
     CDS             complement(<1..716)
                     /gene="eno"
                     /locus_tag="VNN25_18865"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103958.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXH93651.1"
                     /translation="MFKIEELFAREILDSRGNPTIEVDCLLDSGASGRAAVPSGASTG
                     SREALELRDGDKRRYLGKGVANAVENVEIIAKEVEGMDAREQALIDLLMKQIDGTENK
                     GNLGANAILGVSLAVAHAAAQASGLPLFQYLGGPGARTLPVPMMNVINGGAHADNNLD
                     VQEFMIIPAGFDDFSDALRAGAETFHHLKAILHDRKLTTAVGDEGGFAPDLKSNAEAL
                     DLILTAIEKAGYKAGEQIFLG"
     gene            766..1359
                     /locus_tag="VNN25_18870"
     CDS             766..1359
                     /locus_tag="VNN25_18870"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HXH93652.1"
                     /translation="MIDHYRTLATTAESRQKIDRSEFLGIAFPVETDEGFFSELALLT
                     RRHFDATHLCWAFRLFAHGEIRARSADAGEPSGTAGKPILSAIEGAGLYDVAVIVVRW
                     YGGVKLGTGGLSRAYRETAAETLRGASLVDRYVYKRFRIIVPFDQLGVVYRLLDVPHI
                     VLAGEHFGETNEFEIDVRLGRADEFVKMLAEKRITLL"
     gene            complement(1346..1753)
                     /locus_tag="VNN25_18875"
     CDS             complement(1346..1753)
                     /locus_tag="VNN25_18875"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXH93653.1"
                     /translation="MTADGSQTTLLRVAAFLVDALSLSIVLVLPAAILSYTLAWIGGE
                     IKAIQIVWFVALCIVIVGMLFRDGWRGRSLGKQILGLRIVIPGEVPCGYLRSLVRNIV
                     LIVPGLNLAEVVLVIAGKRRVGDRIAKTTITEE"
     gene            1803..2633
                     /locus_tag="VNN25_18880"
     CDS             1803..2633
                     /locus_tag="VNN25_18880"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH93654.1"
                     /translation="MNVRSRVIYSLSATLLLAGCGAVMERRVTNIDRKWPAGAIRHIE
                     VREIDGAINIEAGSGDEITMNATVRSRRKPKDGVENLGYFTTELNGDTLTIGRRNEHQ
                     GFFLFRIGNDTRVDYELHVPPSVALDLRTVNGRIATSGVDGEMRAVTVNGRVNIETPG
                     SSEVEAHTVNGRIEAHFDKSFQGATLKTVNGNVTAVLPLSASFSGDFSQVNGDIEAAF
                     PLNIHSHPGSRRVSGEVNGGRYELRITTVNGDIKIDTSKSSAPPAPPAVPAAPAPPST
                     "
     gene            complement(2659..>2927)
                     /locus_tag="VNN25_18885"
     CDS             complement(2659..>2927)
                     /locus_tag="VNN25_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93655.1"
                     /translation="KFVSDISGQDIKEHKNKPNAAIEAVRNFLRTESGLADIPGWEYI
                     GARYRKFKRELPEIARVGHLNPAKLTYVDYCEMIRRWLDLNARG"
BASE COUNT          555 a          912 c          909 g          551 t
ORIGIN      
        1 ccgaggaaga tctgctcgcc ggccttgtat cccgccttct cgattgcagt caggatcaga
       61 tcgagcgcct cggcgttcga tttaagatcg ggcgcgaacc cgccttcgtc gccgaccgcg
      121 gtcgtgagct tgcggtcgtg aaggatggct ttgaggtgat ggaaggtctc ggcaccggcg
      181 cgaagagcgt cgctgaaatc gtcgaagccg gccgggatga tcatgaactc ctgcacgtcg
      241 aggttgttat cggcgtgggc gccgccgttg atgacgttca tcatcggcac gggcagcgtg
      301 cgcgcgccgg gcccgccgag gtattggaaa agcggcaggc cggaagcctg cgccgcagcg
      361 tgggctacgg ccagcgagac gccgagaatg gcgttcgctc cgaggttgcc tttgttctcc
      421 gtgccgtcga tctgcttcat caggagatcg atgagcgcct gctcgcgggc gtccatcccc
      481 tcgacttcct tggcaatgat ctcgacgttc tcgaccgcgt tggcgacgcc tttgccgagg
      541 tagcgcctct tgtcgccgtc gcggagctcg agcgcttcgc gtgatccggt cgacgcgccg
      601 gagggcacag cggcacggcc cgaagcgccg gagtcgagca gacagtcgac ctcgatcgtc
      661 ggattgccgc gggaatcgag aatctcacgc gcgaaaagct cttcgatctt gaacatgaga
      721 gcgattatat gaggcggtcg gtagttgccg cagaatgctc cagaaatgat cgatcactac
      781 cgcacgctcg ccacgacggc cgaatcccgg cagaagatcg atcgctcgga gttcctcggc
      841 atcgcctttc ccgtcgagac cgacgaaggg ttcttcagcg agctggcgct tctcaccaga
      901 cgccatttcg acgccacaca tctctgctgg gcgttccgtc ttttcgcgca cggcgagatt
      961 cgcgcgcgaa gcgctgacgc gggcgagccc tcgggtaccg cgggaaagcc gattctcagc
     1021 gcaatcgagg gagccggttt atacgatgtc gccgtcatcg tcgtgcgctg gtatggcggc
     1081 gtgaaactcg ggacgggagg cttatctcgt gcgtatcgcg agacggcagc cgagaccttg
     1141 cgcggagcat cgctcgtcga tcgctacgtc tacaaacgct tccgcatcat cgtcccgttc
     1201 gatcagctcg gcgtcgttta ccggctgctc gatgttcctc atatcgtcct cgccggcgag
     1261 catttcggcg aaacgaacga gttcgagatc gatgtgcggc ttggaagggc ggacgagttc
     1321 gtgaagatgc tggccgagaa acggatcact cttctgtaat ggtcgtttta gcgatccggt
     1381 cgccgacgcg gcgcttgccg gcaatgacga ggacgacctc ggcgagattc aagcccggca
     1441 cgatgagcac gatgttgcgc acgagacttc gcagatagcc gcaaggcact tcccccggga
     1501 tcacgatacg aaggccgaga atttgcttgc cgagggagcg gccccgccag ccgtcgcgga
     1561 agagcattcc gacgatgacg atgcacagcg cgacgaacca gacgatctgg atcgctttga
     1621 tttcgccgcc gatccaggcg agcgtatagc tgagaatggc ggcggggagg acgagcacga
     1681 tcgagaggga aagggcgtcc accaggaacg ccgcgacacg caggagtgtc gtttgggagc
     1741 catccgccgt cattggaaac cctttggcct cattctcgta ccaccttgct gcaaggacca
     1801 ctatgaacgt cagatctcgc gttatctatt cactttcggc gacacttctt ctcgccggat
     1861 gtggcgccgt gatggagcgg cgggtcacga acatcgaccg caagtggcca gccggcgcga
     1921 tccgccacat cgaagtgcgt gagatcgacg gcgcgatcaa catcgaagcc ggctccggcg
     1981 acgagatcac gatgaatgcg acggtccgaa gccgccggaa gccgaaggac ggcgtagaaa
     2041 acctcggcta cttcaccacc gagctgaatg gcgacacgct gacgatcggc cgccgcaacg
     2101 agcatcaagg cttcttcctt ttccgtatcg gcaacgacac ccgcgtcgat tacgagttgc
     2161 acgttccccc ctcggtggcg ctcgatctgc gcaccgtcaa tggccgcatc gcaacctccg
     2221 gcgtcgatgg cgagatgcgc gcggtgaccg tgaacggacg ggtgaacatc gagacacccg
     2281 gctcaagcga ggtggaagcg cacaccgtca acggccgcat cgaggcgcat ttcgacaagt
     2341 cgttccaagg cgcgacgctg aagaccgtga acggcaacgt gaccgcggtg ctgcctcttt
     2401 ccgcctcgtt cagcggcgat ttctcgcagg ttaacggcga catcgaagcc gccttccccc
     2461 tcaacatcca ctcgcacccc ggcagccgcc gcgtctccgg cgaagtgaac ggcggccgat
     2521 acgagctacg catcacgacg gtgaacggcg acatcaagat cgacacgtcg aagtcgtctg
     2581 ctcctcctgc gcctcccgcg gttccggcag cgccagcgcc gccgagtacg taagctccga
     2641 tttcatttgc cggacgtctc agccacgtgc attcaggtcg agccatcgcc tgatcatctc
     2701 gcaatagtcg acgtacgtca gtttcgcggg gttgagatgc ccaaccctgg cgatttccgg
     2761 cagctcacgt ttgaacttgc ggtaacgcgc accgatgtac tcccatccag gaatgtcggc
     2821 gagcccggac tccgtgcgga gaaagttgcg caccgcctca attgcggcgt tgggcttgtt
     2881 cttgtgctcc ttgatgtcct ggcccgaaat gtcggacacg aacttct
//
LOCUS       DATLVW010000649         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_438434, whole genome shotgun sequence.
ACCESSION   DATLVW010000649 DATLVW010000000
VERSION     DATLVW010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438434"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VNN25_18890"
     CDS             complement(<1..695)
                     /locus_tag="VNN25_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HXH93656.1"
                     /translation="MNEERSRLTGEPHALARWRKWGPYLADRAWGTVREDYSANGDAW
                     SYFPHDLARSKAFRWGEDGIAGFCDRYQILCWSLALWNERDPILKERFFGLVPGEANH
                     GEDVKECYFHLDAVPSHSYQRLLYKYPQREYPYVQLIEENRRRSSHDPEFELIDTGIF
                     DDGRYFDVIIEVAKEDADSLAIRITAHNRGPERAPLHFIPQLWFRNTWSWSGEPVVSP
                     TITAAGTGGNPAL"
     gene            complement(801..1262)
                     /locus_tag="VNN25_18895"
     CDS             complement(801..1262)
                     /locus_tag="VNN25_18895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXH93657.1"
                     /translation="MTEKSIQETWAPQNRCFGCGPANDKGLRIRSFREGDEFVCDWTP
                     EPHHAAFDNVLNGGIIGALLDCHSNWAATMVLMERNGSAAPPCTVTSDFHVFLKRPTP
                     MAPLRIRAHVAEVTDDRAVVEASIESNGKVTDTCRGTFVAVKEGHPAYHRW"
     gene            complement(1291..1866)
                     /locus_tag="VNN25_18900"
     CDS             complement(1291..1866)
                     /locus_tag="VNN25_18900"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HXH93658.1"
                     /translation="MNYLAHLFLASDSAESLIGNLAGDFVKGRISDALPAGIAAGIRN
                     HRRIDAFTDSHPSVAAFRRVLIPEYGHYARVIADVFFDHFLAVDFNRYAPESLDAFLM
                     RVYATIDPHIDALPGSLRAVYPRMRDEGWLASYRSIDGIGMALFGISRRLSRRPELDP
                     AVHFLTDPDTRPELERLFHEFFPDVIALRGS"
     gene            1951..>2191
                     /locus_tag="VNN25_18905"
     CDS             1951..>2191
                     /locus_tag="VNN25_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH93659.1"
                     /translation="MNAIETHSLRKVYPAPAIKRRRGVPAPAMPPSAEIVAIDSLDLA
                     VAEGEFFGVLGPNGAGKTTLIGILTTRVRITTGEAF"
BASE COUNT          427 a          658 c          717 g          389 t
ORIGIN      
        1 tgaagcgcgg gattgccccc cgtgccggcg gcagtgatcg tcggagaaac gaccggctcg
       61 ccgctccagc tccatgtgtt gcggaaccag agctgaggaa tgaaatgaag cggcgcacgt
      121 tcgggaccgc ggttgtgggc ggtgatgcga atcgcgagcg agtcggcgtc ttccttggca
      181 acctcgatga tgacgtcgaa gtagcggccg tcgtcgaaga ttccggtatc gatcagctcg
      241 aactcggggt cgtgcgagct gcgccggcga ttttcctcga tgagctgaac gtagggatat
      301 tcacgctgcg gatacttgta cagcagccgc tggtaggaat gactcggcac ggcatcgaga
      361 tggaagtagc actccttcac atcttcgccg tggttcgcct cgcccggaac gagcccgaaa
      421 aagcgctcct tgaggatcgg gtcgcgctcg ttccagagcg ccagcgacca gcagagaatc
      481 tggtaacgat cgcagaagcc ggcgatgccg tcctcgcccc agcggaaagc tttcgaccgc
      541 gccaggtcat gcgggaaata cgaccaggca tcgccgttcg cgctgtagtc ctcgcgcact
      601 gttccccatg cgcggtcggc cagatacggc ccccacttgc gccagcgggc gagggcgtgc
      661 ggttcacctg tgagacggga gcgctcctcg ttcatggcga attgcgctcc aaaccgttca
      721 ccacagagac acagaggtca gagagacaca tcaaactctg tgacttccgc gcctctgtgg
      781 tcattcggtt ttccgagcga tcaccaacga tgatacgcag ggtgtccttc cttcacggca
      841 acgaacgtgc cgcggcaagt gtcggtcacc ttgccattcg attcgatcga ggcttcgacc
      901 acggcgcgat cgtcggtcac ctcggcgacg tgcgctcgaa tgcgcagcgg cgccatcggc
      961 gtgggacgtt tcaggaagac atggaagtcg gaggtgaccg tgcacggcgg cgcggcgctt
     1021 ccgttgcgct ccatcagcac catcgtagcc gcccagttgc tgtggcagtc gagcagagcg
     1081 ccgatgatgc cgccgttcag aacgttatcg aaggcagcgt gatgcggctc cggcgtccag
     1141 tcgcagacga actcgtcgcc ttcacgaaaa ctgcggatgc gcaaaccttt gtcgtttgcc
     1201 ggaccgcagc cgaagcagcg gttctgcgga gcccacgttt cctggatgct tttttcggtc
     1261 acggttagat tgtatgcgcg ggagtccgac tcacgacccc cgtaacgcaa tcacatcggg
     1321 gaagaactcg tggaagaggc gctcgagctc cgggcgggta tctggatcgg tcaggaaatg
     1381 cacggctgga tcgagctccg gccggcggga caggcgccgc gagatgccga atagagccat
     1441 cccgattcca tcgatcgacc ggtaggaagc gagccatcct tcgtcgcgca tccggggata
     1501 gacggcgcgc agcgatccgg gaagtgcatc gatgtgggga tcgatcgtcg cgtacacccg
     1561 catcagaaac gcatcgagac tctcgggagc gtacctgttg aaatcgaccg ccagaaagtg
     1621 atcgaagaag acgtcggcga tcacgcgggc gtagtggccg tattcgggga tgagcacgcg
     1681 gcggaatgcg gcgaccgagg gatgcgagtc ggtgaaagcg tcgatgcggc gatgattgcg
     1741 gattccggcg gcgatgcctg ccggcagtgc gtcgctgatc cgtcctttga cgaaatcgcc
     1801 ggccaggttt ccgatcagcg attccgcgct gtccgaggcg aggaagagat gcgcgagata
     1861 gttcaagcgg atacgatagc ggaaggttcg ggggttagct gttagcgaat cgagttagct
     1921 ccgacccccg acacccaacg cccgaaaacc atgaacgcca tcgaaacgca ctcgctgcgc
     1981 aaggtctatc cggcgccggc gatcaagagg cgccgcggtg tgcccgcgcc ggcgatgccg
     2041 ccatccgcgg agatcgtggc catcgactcg ctcgatctcg ccgtcgccga gggcgagttc
     2101 ttcggcgtcc tcggcccgaa cggcgccggc aagacgacgc tcatcggcat cctgacgaca
     2161 cgcgtccgga tcacgacagg ggaggctttt g
//
LOCUS       DATLVW010000650         3868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_362087, whole genome shotgun sequence.
ACCESSION   DATLVW010000650 DATLVW010000000
VERSION     DATLVW010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362087"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VNN25_18910"
     CDS             <1..988
                     /locus_tag="VNN25_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HXH93660.1"
                     /translation="IVARDPQTGQIGVAVQSHWFAVGQIVPWAEAGVGAVATQSFVDP
                     SYGKLGLDLLRTGKPAPDVLRGLLGSDPSCQVRQIGIIDANGNVATFTGSRDIQAAGG
                     IAGQSVGTAAQIGCGTGGGTLNVGHDFAVQANLMSNANIWPSMAKAYAASTGDLADRI
                     LAALDAAQGAGGDIRGKQSAALIVVSAKSTGKPWEDQIFNLRVDDHPEPLKELRRLVA
                     LQRAYNHMNAGDLATEHNDNEGALREYGAAEQIAATTAGIPTSRHAEMIYWHAVALVN
                     MKRVDESLPLFAKAFQMESGWRELTPRLTKAGLLPDDDVLIARIVGVSEQKR"
     gene            complement(1327..1545)
                     /locus_tag="VNN25_18915"
     CDS             complement(1327..1545)
                     /locus_tag="VNN25_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93661.1"
                     /translation="MSALKIVAILLIVAGIAGLIYGKFTYTKTTHDVNLGSVELAVKE
                     KKTVNVPLWAGLGAIVAGGLLFFVPKNT"
     gene            complement(1542..1805)
                     /locus_tag="VNN25_18920"
     CDS             complement(1542..1805)
                     /locus_tag="VNN25_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXH93662.1"
                     /translation="MGLLWFIIIGAIAGWLAGRFMKGHGFGLLGDIVVGIVGAFIGGY
                     LFGAVGVNLGGGLIGSLIVAFIGAVLLLFIVRLFTGRSRRSWS"
     gene            complement(1825..2397)
                     /locus_tag="VNN25_18925"
     CDS             complement(1825..2397)
                     /locus_tag="VNN25_18925"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH93663.1"
                     /translation="MSIVGTTLLSLRLSDLATIRASIAIHGRTRAVVPLMHGSILRQL
                     REAKGRSQADVARSAGISPAQLARLEANQRGLYVEDFVNIAAVLGEKPGNLLPNDMGD
                     IAHLKPLIDRLAAIRPEVLPRVAAIIEKIVLLTEDMTAAGELAAQEPGKAPRTLVSAA
                     GKRKGSRHSGRGGLKNRIQHAEDQSRSDAM"
     gene            2730..>3868
                     /locus_tag="VNN25_18930"
     CDS             2730..>3868
                     /locus_tag="VNN25_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HXH93664.1"
                     /translation="MKRNTFLASIGLATLLLLSASAAFAQISLGTAQNFGVLAGSTVT
                     NTGVTTVNGNVGVSPGSAVTGFPPGIVAGGAIHSNDAVAMQAQNDLTTAYNNIASTPC
                     TVDLTGQDLGGLTLTPGVYCYSSTAQLTGTLTLNALGNPSALFLFKIGSALTTASGSS
                     VTVINNGGSSCNKAYWQVGSSATIGTGSSFAGDILALSSITFTTGSNSSGRALARNGA
                     VTLDTNNINTCGVLACPIITVNPASLPNGTVGIPYNQSVSASGGTAPYAFSVSSGALP
                     TNLILNSVTGAITGTPTTAGTFTFTITATDATGCSGSRLYTVTITSPGCPAIILSPTT
                     LPPGAAQQPYAQTITASGGTAPYTFTITAGSLPAGFSLNATTGLI"
BASE COUNT          752 a         1263 c         1154 g          699 t
ORIGIN      
        1 gatcgtggcg cgcgatcctc agacggggca gattggggtg gcggtgcagt cgcattggtt
       61 tgcggtgggg cagatcgtgc cgtgggcgga ggcgggcgtg ggggcggtgg cgacgcagag
      121 tttcgtcgat ccgtcgtacg ggaagctcgg gctcgatctt cttcgcaccg gcaagcctgc
      181 ccctgacgtt cttcgcggac ttctcggcag cgatccttcc tgtcaggtgc ggcagatcgg
      241 catcatcgac gcgaacggga acgtggccac gttcaccggc agccgcgaca ttcaggctgc
      301 tggcggcatc gccgggcagt cggtgggaac cgcggcgcag atcggctgcg ggaccggagg
      361 cggaacgttg aacgtcggac acgatttcgc cgtccaggcc aacctcatgt cgaacgccaa
      421 tatctggcca tcgatggcga aggcgtatgc agcgtcgacc ggcgatctgg ccgaccgcat
      481 cctcgccgcg ctcgatgccg ctcaaggcgc cggcggcgac atccgcggca agcagtcggc
      541 ggcgctgatc gtggtcagcg cgaagtcgac gggcaagccg tgggaagacc agatcttcaa
      601 tcttcgcgtc gacgatcatc ccgagccgct gaaggagctt cgccgcctcg tcgcattgca
      661 gcgcgcgtac aaccacatga acgccggcga tctcgccacc gagcacaatg ataacgaagg
      721 cgccttgcgc gaatacggcg ccgcggaaca gatcgccgcg accacagccg gaatccccac
      781 cagccgccac gccgagatga tctactggca cgccgtcgcc ctggtgaaca tgaagcgcgt
      841 cgacgagtcg ctacccctct tcgccaaagc gttccagatg gagtccggct ggcgcgagct
      901 cacgccgcgg ttaacgaaag cagggttgct ccctgatgat gacgtgttga ttgcgcggat
      961 cgtgggtgtg agtgagcaga agcgttgatc atccttgtgt cagccgaagc cgccgccttc
     1021 gcgctgccct cgccccggca ggaattggcg gcgcaggacg gtcgaaggat ctccgaatgc
     1081 gacgatctcg ttcgtgcgct ttgacgacga tcggttgcac tgaacacgat ctgcgtaatt
     1141 tgagatcctt cgacgtccta cgccgccgcg cacgcactgc gcaagcatcg cgattcgcgc
     1201 gggcggcttc ggcggtctca ggatgacacc gagcttgatc gcggaattac ctgggagaac
     1261 actcaaccct aaccggcata agacggagcc gggcaacggc tccatctttg ccgcgaacct
     1321 cagcgtttac gtgttctttg ggacgaagaa caacagtccg ccggcgacga tggcgccgag
     1381 gccggcccag agagggacgt tgaccgtctt cttttctttc accgccagct cgacggagcc
     1441 gaggtttacg tcgtgcgtgg tcttcgtata ggtgaacttg ccgtatatga gtcccgcgat
     1501 accggctacg atcaagagga tcgccacgat cttgagcgca ctcaagacca cgatctccgc
     1561 gagcggcccg tgaagaggcg cacgatgaag agcaggagca ccgcgccgat gaacgcgacg
     1621 atcagactgc caatcagtcc gccgccgagg ttcacgccca cagcgccgaa gagatagccg
     1681 ccgataaatg cgccgacgat gccgacgacg atgtcaccaa gaagaccgaa gccgtggcct
     1741 ttcatgaacc ggccggccag ccagccggca attgcgccga tgatgatgaa ccagagtaat
     1801 cccataattt cctgcctttt ccgctcacat agcgtcagat cgtgattgat cttccgcgtg
     1861 ttgaatccga ttcttcaggc cgccgcggcc ggagtgtctg ctgccttttc gtttcccagc
     1921 agcactcacc agcgttcggg gtgctttgcc tggctcttgc gctgccaact caccggctgc
     1981 ggtcatgtcc tcggtcagca ggacaatctt ctcgatgatg gccgcgacgc ggggaagcac
     2041 ctcgggacgg atcgctgcca gccgatcgat gagaggtttc aggtgcgcga tatcgcccat
     2101 gtcgttcggc aggagattgc cgggcttttc accgaggacc gcggcgatgt tcacgaagtc
     2161 ctcaacgtag agtccgcgct gattcgcttc cagccgcgcc agttgcgccg gcgaaatccc
     2221 cgcgcttcgg gctacgtcag cctggctgcg tcccttcgct tcgcggagtt gcctgaggat
     2281 gctgccgtgc atgagtggaa cgactgcacg cgtcctgcca tgaatggcaa tcgaggcacg
     2341 aatcgttgcc aaatcggaaa gtcgcagcga aagcagcgtc gtccccacta ttgacatccg
     2401 gaaaacaggc gtatgctgga atacgttcgt tcagtaagcg cctgatcagc cggtcgcgac
     2461 agacgagcac gagacctctg cagcgttgcc gcgggcctcg cgcctgatcc gccacagcct
     2521 ccatcctcag acgacccgaa aggatgcgat gcccatggcg actcgagccg cgccccactt
     2581 tcctgcccgg catgcggact gcagacgagt ctacttaccc cgcctgacag tatcgagatg
     2641 tctgccggtc atcggcgtcc tgagcaaaat ccagcgaagc aaacgacttc attcactccc
     2701 ccactatgtt cctatcgaaa gaggctgaca tgaaacgaaa cacatttctc gcgagcatcg
     2761 ggctggcgac gcttctcctc ctcagcgcat ccgcggcatt cgcccagatc tctctcggca
     2821 ccgcacagaa cttcggcgtc ctggccggct ctaccgttac caacacgggc gtaacgacgg
     2881 tcaatggaaa cgtcggcgtc agcccgggaa gcgccgtgac cggcttccca cccggcatcg
     2941 tcgccggggg cgcgatccac agcaacgatg cggtggcgat gcaggcacag aacgatctca
     3001 ctacggcgta caacaacatc gcctcgactc cctgcacggt tgatctgacc gggcaagatc
     3061 tgggtggttt gacgctgacg cctggcgtct actgttactc gtccaccgca cagctcaccg
     3121 gcacgctgac gctcaacgcc ctcggcaacc cgagtgcgtt gtttcttttc aagatcggca
     3181 gcgccctgac gaccgcgtcc ggatcgtcgg tcaccgtcat caacaacggc ggaagcagtt
     3241 gcaacaaggc ctactggcag gtcggcagtt ccgcgactat cggaactgga tcatcgttcg
     3301 cgggcgacat cctggccctc tccagcatca cgttcacgac aggctcaaac agctccggac
     3361 gcgcgctggc gcggaacggt gcggtgacgc tcgataccaa caacatcaac acctgcggcg
     3421 tgctggcctg cccgatcatc accgtgaatc cggcatcgct gccgaacggc accgtcggca
     3481 ttccgtacaa tcagagcgta tctgcctcgg gcggaacggc accgtatgcg ttttcggtat
     3541 cgagcggcgc gctgcccacg aatctgattc tgaacagcgt cacgggcgcg atcaccggga
     3601 cgccaaccac cgctggcacg ttcacgttta cgatcaccgc gaccgacgcg accggctgtt
     3661 cgggtagccg gctatacaca gtcacgatca ccagtccggg ctgcccggcc atcatcctga
     3721 gccccaccac gttgccacca ggggctgctc agcagcctta cgcacaaacg atcaccgcat
     3781 cgggtggaac cgctccttat accttcacca tcaccgctgg atcgctgccg gccggcttct
     3841 cgctcaacgc aaccacggga ctgatcag
//
LOCUS       DATLVW010000651         4995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_26064, whole genome shotgun sequence.
ACCESSION   DATLVW010000651 DATLVW010000000
VERSION     DATLVW010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26064"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VNN25_18935"
     CDS             <1..453
                     /locus_tag="VNN25_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93665.1"
                     /translation="WSNKNGQASFNNIPNALAVLSTLNLRNAAGTNFDPANYKQVHDW
                     LLNGTAVNMAYMLSVQMTAMTLNVASGGVNGSRLIYAPGTTSANQFGYASVSAILAEA
                     NANLGANGSTPDGSPARASQEALKNALDAGNNNLNFVQTSPCAFTFAQ"
     gene            690..2501
                     /locus_tag="VNN25_18940"
     CDS             690..2501
                     /locus_tag="VNN25_18940"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXH93666.1"
                     /translation="MSDALPLPRRPHLGYYKKLAKDLLEAATEDDPIALRAWVKRFVP
                     ADQIERIETRIRSADISKLAGAQFFVARAHGFASWPKFAQHVEELQHAGSADAQFEEA
                     ADAIVSGDIGTLRRLLDERPELIRQRSSRDHRSTLLHYVSANGIEDFRQRTPANIVAI
                     TRLLLDAGADVNAESDAYGGHSMTLGLVATSVHPEVAGVQIELMELLLARGATIERVP
                     GEAVRGCLANGRPLAALFLAENGAYLDFENAAGVGSLDRVKDLVATTSAEELGKGFVW
                     ACAYGHNDVVSFLLDSGVDPAAGADVDMTGLHLAAHEGHLDTVKILLAHNAPIEARNV
                     YGGTVLGQTLWSVIHHPRPAHREIVETLLAAGASVGDDWFTGNRSIDELLLRARPEQV
                     EDADPIHELVRRGRRARFEQPEQARELFAEAAAKARQSGAKRELVEALKGIAQIDRDL
                     GRLAAALPFYEEAVSVCREIADPLLLAHTLRHLGDLHHDDGRDDLAEPLYAEALALYR
                     AADAPALDLANALRSIAVATNSAALWQEAFHLYVKTNVPPGAAETATRLARLAHGNGD
                     REGAQEWLRIARAAAEASGEDDVRQRVRSAGEEIGLR"
     gene            complement(2549..3040)
                     /locus_tag="VNN25_18945"
     CDS             complement(2549..3040)
                     /locus_tag="VNN25_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93667.1"
                     /translation="MTRKAKPTVTEAERAATMVKMILRQAAKRVPGLKASHSTTSKRI
                     RGHRTVPKAFIRSMISVVGDLEVLQTMSGFDPAEAQAALQFEAAFRSVVDQMAILQTN
                     LTYTIEAQIAVVAGKALVAYAIAKGFARDGRSRDLAIRIRRLKTALGRKGPRKKKKPH
                     TPA"
     gene            complement(3800..>4995)
                     /locus_tag="VNN25_18950"
     CDS             complement(3800..>4995)
                     /locus_tag="VNN25_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXH93668.1"
                     /translation="PGAVQWWRIANTSSRSTLILKVPDGVTWRQLAQDGVQLTDRNYK
                     SSENADIVLASGNRADLLVKTDPRRQGVTYPINAMLTMDPSIPTTSTQLLLNIKTTAN
                     QPGDKDVPFMDPAPTKLPFLEDIKDADITGRKTIVFATNRNSGTPGFTKQTIDGKQFN
                     GEVGALVLMNNVEEWKIVNESYAGTNIAHPFHIHINPFQITEVFTPNETFGGLHKGAG
                     KVSIAASSAIVKGTGTSFATELRPGWIVNIPGAGRRTVLSIDGNSFTMTSAVNSTTVV
                     TDAEFTFELPRYVFLNSPAPLDGQCYLNPNDPASWKPCAAAPQPPLTERIWWDVFPIP
                     TGLNVTWAGNTSGTKVPGYFKLRSRFVDYSGYYVIHCHILSHEDTGMMTVVYVAPLQP
                     PFSHH"
BASE COUNT          957 a         1510 c         1547 g          981 t
ORIGIN      
        1 tggtcgaaca agaacggcca ggcgtcgttc aacaacatcc cgaacgcgct tgccgtgctc
       61 tccactctca atctccgcaa tgccgcggga acgaatttcg acccggcgaa ttacaaacag
      121 gttcacgact ggctgctcaa cggcaccgcg gtgaacatgg cctacatgct ctccgtgcag
      181 atgacggcga tgacgctgaa cgttgcctcg ggcggcgtca acggctcgcg cctgatctat
      241 gcgccgggaa cgaccagcgc caatcagttc ggctacgcct cggtcagcgc catcctcgcc
      301 gaggcgaacg caaacctcgg cgcgaacggt tcaacgccgg acggctcgcc ggcgcgcgcg
      361 tcccaggaag cgctcaagaa cgcgctcgac gccggcaaca acaacctgaa cttcgtgcag
      421 acctcgccct gcgctttcac attcgcgcag taagcattcc gcccgcggag aagcactcac
      481 gcgaagacgc gaagacgcga aggcgttctt cgcgatcttc gcgtcttcgc gtgaaacgtc
      541 atccctcttc ccaggtacaa tgcacccagc gtttgggacg catccagtcc gcggaaaggg
      601 ccgtgcccac ccactgaatt catcgaaggt acgaaccttc tttcagcctg accgtgcgga
      661 ctccaggcat gcgatgaaag gagtttcgta tgtccgatgc tctccctctt ccacgccgtc
      721 ctcacctcgg ctattacaag aagctggcga aggatctgct cgaggcagct accgaagacg
      781 atccgatcgc gttgcgcgcg tgggtgaagc ggttcgttcc cgccgatcag atcgagcgca
      841 tcgagacgcg gattcggtcg gctgacatca gcaagcttgc cggcgcgcag ttcttcgtcg
      901 cccgtgcgca tggctttgcg agctggccga agtttgcgca gcatgtggaa gagttgcagc
      961 acgccggttc ggcggatgcg caattcgagg aggccgccga cgccatcgtg tccggcgaca
     1021 tcggtacgct acgacgcctg ctcgatgagc gaccggagct gatccggcag cgatcgagcc
     1081 gcgatcatcg atcgacgctt cttcattacg tctccgcgaa cggcatcgaa gactttcggc
     1141 agcgaacgcc ggccaacatc gtcgcgatta caaggttgct gctcgacgcc ggcgccgatg
     1201 tgaatgcgga gtccgacgca tatggcggtc attcgatgac gcttggcctc gttgccacga
     1261 gcgtgcatcc cgaggttgcc ggcgtgcaga tcgagttgat ggagctgctg ctcgcgcgcg
     1321 gcgcgacgat cgagcgcgtg ccgggcgagg cggtgcgcgg ctgtctggca aacggacgac
     1381 cgctagctgc gctgtttctc gctgagaacg gcgcgtatct cgacttcgag aacgctgccg
     1441 gcgttggcag cctcgatcgg gtgaaggatc tcgtggcgac aacgtccgcc gaggagctgg
     1501 gaaaggggtt cgtctgggcg tgcgcctacg gccacaacga cgtcgtctcc tttctgctcg
     1561 actctggcgt cgatcccgcg gctggcgccg atgtcgacat gaccggtctg cacctcgccg
     1621 cgcatgaagg gcacctcgac acggtgaaga tccttctcgc gcacaacgca ccgatcgagg
     1681 ccaggaacgt gtacggcggc accgtcctcg gccagacgct gtggtccgtg attcaccatc
     1741 ccaggccggc gcatcgcgag atcgtcgaga cgcttctcgc tgccggcgcc agtgtgggcg
     1801 acgactggtt caccggcaat cgcagcattg acgagctgct gctccgcgcg cggccggaac
     1861 aggtggagga tgctgatccg atccacgaac tcgtgcgacg cgggcgacgc gcgcggttcg
     1921 agcaaccgga acaagctcgc gaactgttcg ccgaggcggc ggcgaaggcg cggcagagcg
     1981 gcgcgaagcg cgagctggtt gaggcgctca aaggcatcgc ccagatcgat cgcgacctcg
     2041 ggcgcctggc agcagcgctg ccgttctacg aagaggcggt gtccgtctgc cgcgagatcg
     2101 ccgatcctct tcttctcgcg cacactcttc gccacctcgg cgatcttcat cacgacgatg
     2161 gccgcgacga cctggccgaa ccgttgtacg ccgaggcact cgcgctgtac cgggctgcgg
     2221 atgcgccggc gctcgatctg gcgaacgcgc ttcgcagcat cgcggttgcc accaacagcg
     2281 ctgcattgtg gcaagaagcg tttcatctct acgtgaaaac gaacgtcccc cctggcgccg
     2341 ccgaaacggc cacgcggctt gccaggctgg cgcatgggaa tggcgatcgc gaaggcgcgc
     2401 aggaatggct tcgcatcgcg cgcgccgccg ccgaggcgtc aggcgaggac gacgtgcgtc
     2461 aacgggtgcg gtcggcgggg gaggagatcg ggttgcgcta aatccgggtc tcctcttcgc
     2521 gtcttcgcgc cttcgcgtga gaaagcaatc acgccggagt gtgtggtttc ttcttcttgc
     2581 gcggaccttt tcggccgagg gcggtcttga ggcggcgaat tcggatcgcc aagtctcgac
     2641 tgcgcccgtc gcgcgcgaac cctttggcga tggcgtaggc caccagtgct ttcccagcca
     2701 caacggcaat ctgcgcttcg atcgtgtacg tgaggttggt ctgcaggatt gccatctgat
     2761 cgacgacgga acggaacgcc gcttcgaact gcagcgccgc ctgcgcctcc gccggatcga
     2821 atccgctcat tgtctgtagt acttccaggt cacccaccac gcttatcatc gaacggatga
     2881 acgccttcgg gacagttcga tgccctcgga tgcgcttgga cgtcgtcgaa tgagacgctt
     2941 tcagtccggg aacgcgcttt gcggcctggc gaagaatcat cttcaccatc gtcgctgccc
     3001 gttccgcctc tgtaactgtc ggtttcgctt tgcgagtcac cttttccctc cttgaagatc
     3061 tgcaggaccc ttttgaaggt ttgacgcgct gactttcaaa aggttcctgg cacgtggagg
     3121 acacggccag gttcgagaag gctctcgggc tcgtgaaggt ctgggcgtca gatcttgaaa
     3181 ggtcaaaacg cctttcgaag aggtggcgca cagccttgga agggtcaaaa tgccttttca
     3241 agatctgggc gccagatctt cgaaggtcga aacacctttt gaagaggagc cggccgcctt
     3301 tgaagagaca aaacgtcctt tcaagatctg ggcgccagat ctcaaaaggt caaattccct
     3361 tccgaagatc taaacgccag atcttgaaag gccaaaaacc cttttgaaga tcagaacgcc
     3421 agatcttgaa aggccaaaaa cccttccgaa gatctgggcg tcagatcttg aaacctcaaa
     3481 atcacctccg aagatctgga cgccagatct tgaaaggtca aaattccttc tgaagatctg
     3541 aacgtcagat cttgaaaggg caagaacgct tttgaagatc aggacgccag atcttgaaaa
     3601 gacaaaacgc ctgttgaaga tcagtgaagt agatcttgaa gaggacgatc ggcagattga
     3661 ggagcccaag gcgattgatc aaaaaggcgc cgggcgagcc ggagcagaaa gaactgtccc
     3721 tccgacaccc gaatcccgat ccgcgacgcc cgcacgcaca gggcgcggag aaaattcccc
     3781 gcgcccctcg cgtgaaaggt cagtggtgcg agaacggcgg ctgcagcggc gcgacgtaga
     3841 ccaccgtcat catgccggtg tcctcatgcg agaggatgtg gcaatggatg acgtagtagc
     3901 cggagtagtc gacgaagcgg ctgcgcagct tgaagtaacc ggggaccttt gtgccgctgg
     3961 tgttgccggc ccaggtcacg ttgaggccag tggggatcgg gaaaacgtcc caccagattc
     4021 tctcggtcag cggcggttgc ggagctgccg cgcacggctt ccagctcgcc ggatcgttcg
     4081 ggttgaggta gcactgcccg tcgagcggcg cgggtgagtt gaggaagacg tagcgaggaa
     4141 gctcgaaagt gaactcggcg tccgtcacga cggtcgtcga attgacggca gaggtcatcg
     4201 taaacgaatt gccatcgatg ctgagcacgg ttcttcttcc cgcgcccggg atgttcacga
     4261 tccagccggg acggagctcg gtggcgaagc tggtgccggt gcctttcacg atcgccgagc
     4321 ttgcggcgat gctgaccttt cccgcgcctt tatgaagacc accgaatgtt tcattcggcg
     4381 tgaacacctc ggtgatctga aacggattga tgtgaatgtg gaacggatgg gcgatattcg
     4441 tccccgcgta gctctcgttg acgatcttcc actcctcgac attgttcatc agaacgagtg
     4501 cgccgacctc tccgttgaac tgctttccgt cgatcgtctg cttcgtgaag ccgggcgtcc
     4561 ctgaattcct gttcgttgcg aagacgatcg tctttcgtcc cgtgatgtcg gcgtctttga
     4621 tgtcctccag gaacggcagt ttcgtgggcg ccgggtccat gaaggggacg tctttgtcgc
     4681 cgggctggtt ggcggtggtc ttgatgttca gcagcagttg cgtactggtt gtgggaatcg
     4741 acggatccat ggtcagcatc gcgttgatcg ggtaggtcac accttgtctc cgcggatccg
     4801 ttttcaccag cagatcggcg cggttgccgg aagccagcac gatgtccgcg ttttcgctcg
     4861 acttgtaatt cctgtcggta agctgcacgc cgtcctgcgc cagttgtctc caggtcacgc
     4921 cgtccggtac tttgagaatc agcgtggagc ggctcgacgt gttggcgatc cgccaccatt
     4981 gcacggcgcc cggtt
//
LOCUS       DATLVW010000652         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_72174, whole genome shotgun sequence.
ACCESSION   DATLVW010000652 DATLVW010000000
VERSION     DATLVW010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72174"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..321
                     /locus_tag="VNN25_18955"
     CDS             172..321
                     /locus_tag="VNN25_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXH93669.1"
                     /translation="MLWTIAVILLALWALGLVTSYTIGGYIHVLLVVAVIVAVIRLIQ
                     GRRVL"
     gene            433..786
                     /locus_tag="VNN25_18960"
     CDS             433..786
                     /locus_tag="VNN25_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXH93670.1"
                     /translation="MTATLPISFDLVPVASGPAGFRREDTGTETLLRLEGALDALSTP
                     ATRAVLDTLVNDRRSPVAIDMSALHSIDSSGVGAVVSLYKRVVAQGGKLSVRGLRGQP
                     LAIFQLLRLDHVLAA"
     gene            complement(804..1745)
                     /gene="nhaR"
                     /locus_tag="VNN25_18965"
     CDS             complement(804..1745)
                     /gene="nhaR"
                     /locus_tag="VNN25_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HXH93671.1"
                     /translation="MLFEKPTYRTYGRPMEWLNYHHLLYFWMVAKEGSIVRASKELHL
                     AHPTISGQIRRLEEVLGEKLFVRRGRDLVLTDVGQLAFRYANDIFALGGELLDAVKGR
                     GGQPIRLVVGVSDVLAKSIVHRILEPALRLAEPVRLVCREDRSMDAFMADLGVHAVDV
                     VLSDAPAGTDTSVRAFSHLLGECGAVALAAPAMAKALRKGFPASLNGAPFLLPAATST
                     LRRALDAWFDGRDIRPKIVAEFDDAALATVLGEAGLGVFAVPEVVEEEIRRRYQVRRV
                     GRLDGLRQRFYAISVERKIRHPAVAAICEAARKHIFA"
     gene            1798..1947
                     /locus_tag="VNN25_18970"
     CDS             1798..1947
                     /locus_tag="VNN25_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93672.1"
                     /translation="MQTAQKMTAQGVRDLNHTGPKEKPTPIDAGTTTAQPSPPVAAAP
                     ASTDK"
     gene            complement(1993..>2534)
                     /locus_tag="VNN25_18975"
     CDS             complement(1993..>2534)
                     /locus_tag="VNN25_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HXH93673.1"
                     /translation="SDYDREKLQERLAKLVGGVAIVKVGAATESEMREKKARVEDALH
                     ATRAAIEEGILPGGGVALIRAAASLADLKFRDDRQYGVSVIRRAIEEPLRQIATNAGH
                     EGSIVVEKVKNGKGAFGFNAASEEYEDLIKAGVIDPAKVVRAALQNAASVASLMLSTE
                     ALITDLPKRKAGAAASPLA"
BASE COUNT          446 a          859 c          796 g          433 t
ORIGIN      
        1 agtgttttcc ccgatcctga gacaggctgg gatcgcgccg cccggcgggc gaccgacccg
       61 atcgggaaat cccttacaag cgacgctggt cttccggctt ggcgctggga agcatcagcg
      121 cgtattcctg aggcggcatc attgccacga cagacaaaag gagagaagaa aatgctttgg
      181 acaattgctg tgatcttgct ggctctgtgg gcgctcggat tggtaacctc ttacacgatc
      241 ggcggctaca tccacgtcct cctcgttgtg gcagtgatcg tcgccgtcat tcgcttgatt
      301 cagggaagac gagtcttgtg acggctctcg aagagcatcg cagccgataa tgcttacctt
      361 ctttggacta cgcctccggt ctcgggcctc gagagaccag aaaagacacc cgtgatcacg
      421 tcaggcacca ttttgaccgc cactctcccg atctcgttcg acctagtccc ggtggctagc
      481 ggccccgcgg gattccgccg cgaagacact ggaacggaga cgctcttgcg gctcgaaggg
      541 gcgcttgatg cgctctcgac cccggccacg cgtgcggtgc tcgacactct ggtcaacgat
      601 cggagatcgc cggtcgcgat cgacatgtcg gccctgcact cgatcgacag ctcgggcgtc
      661 ggcgcggtcg tctctcttta caagcgcgtc gtcgcgcagg gcgggaagct cagcgtgcgg
      721 ggtctcaggg gccagccact cgcgattttt cagctcctcc gtctcgatca cgtcctggct
      781 gcatgagcat cggccgtgac cgatcacgcg aagatgtgct tgcgcgcggc ctcgcagatc
      841 gcggcaacgg ccggatgcct gatcttgcgc tcgaccgaga tggcgtagaa gcgctggcgg
      901 agtccatcga ggcggccgac ccgccggacc tggtaacgtc ggcggatctc ttcctcgacc
      961 acctccggaa cggcgaacac tccaagtccc gcctcgccga gcacggtcgc gagcgcggcg
     1021 tcgtcgaact cggcgacgat cttgggtcgg atgtctcgtc cgtcgaacca ggcgtcaagc
     1081 gcgcgccgca gcgtcgacgt cgccgccggc aacaaaaacg gggcgccgtt cagcgaagcg
     1141 ggaaagccct tccgcagagc cttggccatc gccggcgcgg ccagcgccac ggccccgcac
     1201 tcaccgagca agtgactgaa tgcgcgaacg gacgtgtccg taccggccgg ggcgtcgctc
     1261 aggaccacgt cgaccgcgtg cacgccgaga tcggccatga acgcgtccat cgagcgatcc
     1321 tcacgacaga cgagacgcac cggctcggcg agacgaagcg ccggctccaa gatcctgtgg
     1381 acgatcgact tggccaggac gtcggagaca ccgaccacca gacggatggg ctggccgccg
     1441 cgccctttca ccgcgtccag gagctcgccc cccagggcga agatgtcatt ggcgtatcga
     1501 aacgccagct gtcccacgtc ggtgagcacg agatccctgc cccgccgcac gaatagcttc
     1561 tctcccaaca cctcttcgag ccgacggatt tggccgctga tcgtcgggtg cgccaggtgc
     1621 agttctttgc tggcgcgcac gatgctgccc tccttcgcga ccatccagaa gtagagaagg
     1681 tggtggtagt tcagccattc catcggacga ccatacgtcc gatacgtcgg tttttcgaac
     1741 agcatccggc ggaacgagca gcgggtgaga ccggcggcgg tgccctacgc tgaaggaatg
     1801 caaacagctc aaaagatgac cgcccagggt gtacgcgact tgaaccacac tggcccgaag
     1861 gaaaagccga caccgatcga cgccggcacc acgacggcgc agccgagccc gccggtggcg
     1921 gccgcaccgg caagcacgga caaatgagct gcgcgcgctg ggctcaggcc acccacgcgc
     1981 gcacaatgcc ggtcaggcga ggggcgacgc ggccgcgccg gccttccgct tcggcaggtc
     2041 ggtgatgagg gcctcggtgc tcagcatcag cgacgccacc gatgcggcgt tctgaagcgc
     2101 ggcgcgcacc accttggcgg gatcgatcac acccgccttt atcagatctt cgtactcctc
     2161 gctggccgca ttgaagccga acgcgccctt gccgttcttc accttctcga cgacgattga
     2221 gccttcgtgc ccggcgttgg tcgcgatctg gcgcaacggc tcctcgatgg cgcggcggat
     2281 gacgctgacg ccgtactgtc gatcgtcccg gaacttgagg tcggccagcg acgcggctgc
     2341 acggatcagc gcgacgccac ctccgggcag gatgccctct tcgatggcag cgcgggtcgc
     2401 gtgcagcgcg tcctcgaccc gggctttctt ctcgcgcatc tccgactcgg tggccgcgcc
     2461 caccttcacg atggcgacgc ctcccaccag cttggccaac cgttcttgca gcttctcgcg
     2521 gtcgtagtcg gagg
//
LOCUS       DATLVW010000653         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_362253, whole genome shotgun sequence.
ACCESSION   DATLVW010000653 DATLVW010000000
VERSION     DATLVW010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362253"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VNN25_18980"
     CDS             <1..1177
                     /locus_tag="VNN25_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93674.1"
                     /translation="SDFKFVQAGFSVIRFTKAGKIVWQSTHTICETGLTDCGTQVLRY
                     GAIAMDGDRFVVIGTIFDNAKPSLSDSIGIGIDDGGKVVWSKRYQTTRAIGRAMGIVP
                     LSKPGQFLVASRNAAETDTWLYGIDRTGKLTGLEMRFFDKFVQRLCSLPGQGIFVLGT
                     NNDDASHPLGWILNIDPATGKPAWERTYDRHDGTMLRFHDAADGKGIVTAIGNAGAIA
                     KPFLATLANNSAPDAGNVRASFRPTSGESTIFLNGIANVPSPTSTSSICGEFDGAAWH
                     IAIDDAVKILWQKKYNHGRSSASLTPIVFPNAATVVAGGQAIVPGDPLRGLLVRSKTA
                     IGSPASFACGEETNAVFEPANLTSKQIKSEFKDLAMIASDIFVNQATIIEVETGCLP"
     gene            complement(1224..>2084)
                     /locus_tag="VNN25_18985"
     CDS             complement(1224..>2084)
                     /locus_tag="VNN25_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXH93675.1"
                     /translation="ADAPRDVDEVLNYVAAMNHGLARLPKLPVSVRLIREIHEKLLHG
                     VRGSHMTPGELRRTQNWIGPSGCTLSEATFVPPPPSEVQNALGALEKFIHRSDTLPLL
                     IKIGLAHAQFETIHPFLDGNGRVGRLLITFLLCERGVLHKPVLYLSHYFKRHRSRYYD
                     LLQNVRDRGDWETWLAFFLRGVAQVSTQATDTARRILALREEHRYIITNHLGRAAANG
                     YRVLERLYERPILSVEDIRQLTGTTFPAANQLAQKLVEQGLVTEMTGQRRHRRFRYDA
                     YIRLFDEETI"
BASE COUNT          408 a          652 c          646 g          378 t
ORIGIN      
        1 ttccgatttc aagttcgtcc aggccggctt ctccgtcatc cgcttcacga aggcgggcaa
       61 gatcgtgtgg cagagcacgc atacgatttg cgagaccggc ctcaccgatt gcggcacgca
      121 ggtgctgcgg tacggagcga tcgcaatgga cggcgaccgg ttcgtcgtca tcggcaccat
      181 cttcgataac gccaaaccca gcctgtccga ttcgatcggc attggcatcg acgatggcgg
      241 caaggtcgtt tggagcaagc ggtatcagac gacgcgcgcc atcggccggg ccatgggcat
      301 cgtgccgctg tcgaagccgg gccagttcct cgtcgcgtcg cgcaacgctg ccgagacgga
      361 cacctggctc tacggcatcg acagaaccgg caagctcact ggcctggaga tgcggttctt
      421 tgacaagttc gtgcagcgcc tgtgctcgct tccggggcaa ggcatcttcg tactcggcac
      481 gaacaacgac gatgcgtccc atccgctggg atggatcctg aacatcgatc cggcaaccgg
      541 aaagcctgca tgggagcgca cgtacgatcg gcacgacggc acgatgttgc gcttccacga
      601 cgctgccgac gggaagggca tcgtcacggc aatcggcaac gccggcgcga tcgcgaaacc
      661 gttcctcgcc acgctggcca acaacagcgc gccagacgcc ggcaacgtcc gagcgtcgtt
      721 ccggcccacc tcgggagaat cgacgatctt cctcaacggc atcgccaacg ttccgtcgcc
      781 aacctcgaca tcgtcgatct gcggcgaatt cgacggcgcc gcctggcaca tcgccatcga
      841 cgacgcggtc aagatcctct ggcagaagaa gtacaaccac gggcggtcga gcgcgtcgct
      901 cacgccgatc gtgttcccga acgccgccac cgtcgtcgcc ggcggccaag ccatcgtccc
      961 cggcgatccg ctgcgcggcc tgctcgtgcg atcaaagacc gccatcggca gtcccgcgtc
     1021 cttcgcgtgc ggcgaggaga cgaacgccgt cttcgagccg gcgaacctga cgagcaagca
     1081 gatcaagtcg gagttcaagg acctcgccat gatcgccagc gatatcttcg tgaatcaggc
     1141 gacgatcatc gaggtcgaaa cgggttgcct gccctaacga tcactgaaaa atgcgactgc
     1201 acgccagagt cgctcacgga caatcagatc gtttcctcat cgaacaacct tatgtacgcg
     1261 tcgtaccgaa atcggcgatg acgtctctgg ccggtcattt cggtgactag tccctgctcg
     1321 acgagtttct gcgcgagttg atttgcggct gggaaggtcg tgcccgtgag ctgacggatg
     1381 tcttcgacgg agaggatggg gcgttcgtag agacgctcca ggacgcgata gccgttggcg
     1441 gcagcgcgac cgaggtggtt ggtgatgatg tagcgatgct cttctcgaag agcgaggatg
     1501 cggcgtgcgg tatcggtggc ttgcgtactc acctgggcca ccccgcgcag gaagaacgcc
     1561 agccacgtct cccagtcgcc gcgatcgcgg acgttctgga gcaggtcgta gtacctcgat
     1621 cgatggcgct tgaaatagtg agagaggtag agcaccggct tgtgcagaac gccgcgctcg
     1681 caaagcagga aagtgatgag cagccgtccg actcggccgt tgccgtcgag aaatggatga
     1741 atggtctcga actgcgcatg ggccagaccg atcttaatga ggagaggtag cgtgtcgctt
     1801 cggtggataa acttctccag cgcacctagc gcgttctgta cctccgatgg tggcggtggc
     1861 acgaacgtcg cctcggacag cgtgcaccca ctcggcccga tccagttttg cgtcctccgc
     1921 agttcgcccg gcgtcatgtg ggaaccgcgc acgccatgaa gcagtttctc gtgtatctcg
     1981 cggatcaggc gaaccgagac cggcagcttt ggcaaacgcg ccagaccatg gttcatcgcc
     2041 gcgacgtagt tgagcacttc gtccacatcc cgtggagcat ctgc
//
LOCUS       DATLVW010000654         3920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_481247, whole genome shotgun sequence.
ACCESSION   DATLVW010000654 DATLVW010000000
VERSION     DATLVW010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481247"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VNN25_18990"
     CDS             <1..1312
                     /locus_tag="VNN25_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH93676.1"
                     /translation="NVAVDDVSLAFAPGEIVGLVGENGAGKTTLMSIAAGELAADEGT
                     IDERTSAGIVHQHFLLVSEFTIAENLALAMWGRDAESIIRDSGIALRDVLRRVADISV
                     GEKAKLELIKAVARHPRFLILDEPTSVLAPAEARELFVVMRQLADNGTTVVFISHKIP
                     EVLEVATRVVVMRRGRIVADGGTMTASELAEAMVERRQVEQRQQVERRSDSPVRQTAA
                     VSRTGESDLRLPMLQLEDGLAVHAGEIVAIIGVAGNGQSELATSLRDSLRGRVAHIPE
                     DRARDGLIAEMTIAENIALAEPHWRPRIAAQTASALIERFDIRAQSPLQRAGSLSGGN
                     QQKVILARELERKPEIIVAAEPSRGLDIEATRFIHDELRAAAMRGAAILLITSDLDEA
                     FALGDAIHVIYRGKLSDRLTPAEAAERAPQLMAGVERRESGVGA"
     gene            1309..2316
                     /locus_tag="VNN25_18995"
     CDS             1309..2316
                     /locus_tag="VNN25_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93677.1"
                     /translation="MSGKLRIPISVSRIGIALIAATTAIAGLALCGIAFGRSPGELLA
                     ILASGSIGSSFALQGTLLKSVPLLLTGLSVAVAFRAGVWNIGGEGQFLVGALGAFLGA
                     RYGVIASLMAAVLAGAIWASIATAMRLWRNAPEVLTTILLNFIAIHLLGCAVNGPLQE
                     ASGKYPQSDAVPAGATLPLLANLELHAGVVIAVLVAVAAWYLLYQTAEGLRLRATGFN
                     RSAAKWAGVNVDGQLIRAMAISGAMAGLAGGIELLGVTHRLFGRFAAGYGYAGIAVAL
                     LAQLHPLGTIASALFFGALTTGAGELQRTAGISSMVATLAQAIVILAMIAFMRRSKAS
                     S"
     gene            2313..3164
                     /locus_tag="VNN25_19000"
     CDS             2313..3164
                     /locus_tag="VNN25_19000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93678.1"
                     /translation="MTSQLLASIPLAIVASTPLLLAVLGELVVERAGMINLGIEGMML
                     TAAMTAVVAAQWTHSITIGFLAGIAGAAAIGALFGLFAIRFAVDQIVTGTAIVLLAQG
                     LTGVVYRQLQQRAIFLQPVPRFDTDVVVPLAWIVIPIVLAFLLWRTRFGLRLRACGEL
                     PAAVTAVGASVAMHRWIALAIESVLTGIAGAYLALALSSGFAENMTSGRGFIALSIVI
                     FGRWKFKGALLGTAVFGIAAAAQYALQASGRGIPFHLLLAMPYVVTLLILCGIAGRVR
                     APESLGR"
     gene            complement(3300..>3920)
                     /locus_tag="VNN25_19005"
     CDS             complement(3300..>3920)
                     /locus_tag="VNN25_19005"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93679.1"
                     /translation="EMNTRLQVEHPVTEEVAGVDLVRAQLAVAGGAPLPWRQDDLRQR
                     GHAIEVRIYAEDPDDRFLPQSGVIRFYREPAGPGVRVDAGVGQGTSIGVSFDPMLAKL
                     ICSGPTRDAAIDRLARALRDYIVLGTKTNISWLRRVVQYPAFREGLVSTRFIADHEDS
                     LRRTMPDEVPAIAAVLSSTTQREQRTTGGKAPGIGSVWDSIGEWGR"
BASE COUNT          630 a         1308 c         1263 g          719 t
ORIGIN      
        1 caacgtcgcc gtcgatgacg tctcgctcgc gttcgcgccc ggcgagatcg tcggactcgt
       61 cggggagaac ggcgcgggaa agacgacgct gatgtcgatc gccgccgggg agttggctgc
      121 ggacgaaggg acgatcgatg agcgcacctc ggcaggcatc gtccaccagc actttctgct
      181 cgtcagcgaa ttcacgatcg cggagaacct cgcgctggct atgtggggac gcgacgcgga
      241 gtcgatcatt cgcgactccg gcatcgcgct gcgtgacgtt ctccggcgcg tggcagacat
      301 ctccgtcggc gagaaggcga aactggagct gatcaaggcg gtcgcgcgcc atccgcgctt
      361 tctcatcctc gacgaaccga cctcggtgct cgcgcccgcg gaggcacgcg agcttttcgt
      421 cgtcatgcga caactggcgg acaacggcac gaccgtcgtc ttcatcagcc acaagatccc
      481 cgaggtgttg gaagtagcaa cgcgggtggt ggtgatgcgg aggggaagaa tcgttgccga
      541 tggcggaacg atgacggctt cggagctggc cgaggcgatg gtggagcgaa gacaggtgga
      601 acagcgtcag caggttgaac gtaggtcgga ctctccagtc cgacaaaccg cggcagtcag
      661 tcggactgga gagtccgacc tacgtttgcc aatgctacag cttgaggacg gccttgccgt
      721 tcacgcgggc gagatcgtgg ccatcattgg cgtagccggc aacggtcaat cggaattggc
      781 gacgtcgctg cgcgactcgt tgcgcggacg tgtggcgcac atcccggagg atcgcgcgcg
      841 cgatgggctc atcgcggaaa tgaccatcgc ggaaaacatc gccctcgcag aaccgcactg
      901 gcggccgcgc attgccgcgc agaccgcttc ggcattgatc gagcgattcg acattcgcgc
      961 gcagtcgccg ctccagcgtg ccggatcgct ctccggcggc aatcaacaga aagtcatcct
     1021 cgcgcgcgag ctggagcgga agccggagat catcgtggcc gccgagccgt cgcgcggact
     1081 ggacatcgaa gcgacgcgct tcattcacga cgagctgcgc gctgccgcca tgcgcggcgc
     1141 cgccatcctc ctcatcacct ccgatctcga cgaagcattc gcgctcggtg acgccatcca
     1201 cgtcatctac cgcgggaaac tcagcgatcg tttgacgcca gccgaggcgg cggagcgagc
     1261 gccgcaactg atggcgggtg tcgagcggcg agagtcgggg gtcggagcgt gagtggcaaa
     1321 ctgcgcattc cgatttccgt ttcgcgaatt ggcatcgccc tcatcgccgc gacgaccgcg
     1381 atcgccggcc tggcactttg cggcatcgcc ttcggccgat cgcccggaga gttgctggcg
     1441 attctcgcca gcggctcgat cggctcttca ttcgcgctgc agggaacgct gctcaagtcc
     1501 gtgccgctcc tgctcaccgg gctctccgtc gccgtcgcgt ttcgcgccgg cgtctggaac
     1561 atcggcggcg agggtcagtt cctcgtcggc gcgctcggcg cttttctcgg cgctcgctac
     1621 ggtgttatcg catcgctgat ggcagcagtt ctggccggcg cgatctgggc gtcgatcgca
     1681 accgcgatgc ggctttggcg caacgccccc gaggtgctga cgactatcct cctcaacttc
     1741 atcgccatcc atctcctcgg ctgcgccgtg aatggcccgc tgcaggaagc gagcggcaag
     1801 tacccgcaga gcgacgcggt gccggccggc gctaccttgc cgctgctcgc caatctcgag
     1861 ctgcacgccg gagtcgtgat cgcggtcctc gttgcggtcg ccgcctggta cctgctgtat
     1921 cagaccgccg aggggcttcg cctgcgtgcg acaggattca accgttccgc ggcgaagtgg
     1981 gcaggcgtca acgtcgacgg gcagctcatc cgagcaatgg ccatctcggg agcgatggcc
     2041 ggattggcgg gcgggatcga acttctcggc gtcacgcatc gcctcttcgg gcgcttcgcc
     2101 gcaggctacg gctacgcggg aatcgccgtt gctcttctcg cgcaacttca ccccctgggt
     2161 accatcgcct cggcactctt cttcggcgct ctgacgaccg gcgccggtga actgcagcgc
     2221 accgccggca tctcgtcgat ggtggcgacg ctggcgcagg cgatcgtcat cctggccatg
     2281 atcgcgttca tgcggcggag caaggcatct tcatgacttc gcaactcctg gcttccattc
     2341 cgctggcgat cgtcgcgtcg acgccgctgc tgctggcagt gctcggcgag ctcgtcgtcg
     2401 agcgcgccgg catgatcaac ctcggcatcg aaggaatgat gctgacggcg gcgatgaccg
     2461 ctgtggtcgc ggcacagtgg acacacagca tcacgatcgg atttctcgcg ggcatcgctg
     2521 gtgccgcggc gatcggcgcg ctcttcggtc tctttgccat ccgcttcgcc gtcgatcaga
     2581 tcgtgaccgg caccgccatc gtcctgctcg cccagggcct gaccggcgtc gtctatcgcc
     2641 aactgcagca gcgcgcgatc tttcttcagc cggtgccgcg cttcgatacg gacgtcgtcg
     2701 tgccgctcgc ctggatcgtc attccgatcg tgctggcgtt cctcctctgg cgtacccgat
     2761 tcggacttcg cctgcgcgca tgcggtgaac tgcccgcagc ggtaacggcg gtcggagcct
     2821 ccgtagccat gcatcgctgg atcgcgctcg cgatcgagag cgtgctcacc ggcatcgcgg
     2881 gcgcctacct cgcgctcgcc ctctccagcg gcttcgccga aaacatgacg tcaggccgcg
     2941 ggttcatcgc cctctcgatc gtgatcttcg gccgctggaa gttcaaaggc gcgctcctcg
     3001 gcaccgctgt cttcggcatc gccgccgctg cgcaatacgc actccaagcc agcggccgcg
     3061 gcatcccgtt ccacctgctg ctagcgatgc cctacgtcgt gacgctgctg atcctctgcg
     3121 gcatcgcagg aagagtgcgc gcgccggaaa gtttggggcg gtgaatctct cagcacagac
     3181 aagagtgtct gtgccacatc acttcgggcc actggcgaat ctgaggctga taaccagctc
     3241 gcccttcaag cgatggttgt gtggcacaga cactcttgtc tgtgctcgga ccaagtgccc
     3301 tatcttcccc attcgccgat cgagtcccac acgcttccga tgccgggcgc ctttcctccc
     3361 gtcgtgcgtt gctcccgctg cgtcgttgag gacagcactg ctgcgatcgc gggaacttcg
     3421 tccggcatcg tgcggcggag cgagtcctcg tgatcggcga tgaatcgtgt cgagacgagg
     3481 ccttcgcgaa acgcgggata ctggacgacg cgtcggagcc aggagatgtt ggtcttcgtg
     3541 ccgaggacga tgtagtcgcg tagggcgcgg gcgaggcgat cgatggcggc atcgcgcgtc
     3601 gggccagagc aaatcagctt ggccagcatc ggatcgaagc tgactccgat cgacgtgccc
     3661 tgaccgacgc ccgcatccac gcgcacacca gggccggcgg gctcgcgata gaagcggatc
     3721 acgccgctct gcggaagaaa acgatcgtcc ggatcttccg cgtagatgcg cacttcgatg
     3781 gcgtggccac gctggcgaag atcgtcctgc cgccatggca acggcgcacc tcccgccacc
     3841 gcaagctgcg cgcggacgag atcgacaccc gcaacctcct cggtcaccgg atgctccacc
     3901 tgcaaccgcg tgttcatctc
//
LOCUS       DATLVW010000655         5304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_524466, whole genome shotgun sequence.
ACCESSION   DATLVW010000655 DATLVW010000000
VERSION     DATLVW010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524466"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..258)
                     /locus_tag="VNN25_19010"
     CDS             complement(91..258)
                     /locus_tag="VNN25_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93680.1"
                     /translation="MCDNTSSRTGESDLRNPFENASATLRALAAAGGFTIEQRWTDRN
                     GYFADLLMVAR"
     gene            complement(297..464)
                     /locus_tag="VNN25_19015"
     CDS             complement(297..464)
                     /locus_tag="VNN25_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93681.1"
                     /translation="MCDNTSSRTGESDLRNPFENASDTLRALANAGGFTIEQRWADRN
                     GYFADLLMVAR"
     gene            complement(503..1519)
                     /gene="egtD"
                     /locus_tag="VNN25_19020"
     CDS             complement(503..1519)
                     /gene="egtD"
                     /locus_tag="VNN25_19020"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXH93682.1"
                     /translation="MQPSLAVAAPPVTRGRFTIQNAAAAPVSNFAADVRRGLTATPKF
                     LLPHYFYDALGSALFSAICELPEYYVTRAEDEILRERSAEIAASFGPSVRLVELGSGS
                     ATKTRHLISAIVERQATLEYIPVDVDAGILTASAHELLTDYPQLSIHAVCGDFREPAA
                     LLQPHLPPSDERTIVLFLGSSIGNLDPSESGAMLANLRTALASGDALFLGADLRKSAS
                     ILEPAYDDPLGVTAAFNLNLLGRINRELGGTFDLASFAHRAFYNEAAGRIEMHLVSRK
                     AQRVLIDGYDVVFAKGESIHTENSHKYDDATLRALATAGGFTVEQLWTDRNGYFADLL
                     MVAR"
     gene            complement(1675..2055)
                     /locus_tag="VNN25_19025"
     CDS             complement(1675..2055)
                     /locus_tag="VNN25_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXH93683.1"
                     /translation="MKASSPTFTLVAGHYAIVRLHADAAIPSWAHGAFLSITRTHDEL
                     SVVCDAANVPDVVADRGWRCLKLAGPFPLDQTGVAAAFTRVLADAGVALFVVSTYDTD
                     YVLVRDGVLEHASAALRAAGYVVR"
     gene            complement(2062..2436)
                     /locus_tag="VNN25_19030"
     CDS             complement(2062..2436)
                     /locus_tag="VNN25_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93684.1"
                     /translation="MFFKTRKRVLLLEDDPSMQRLVAKILGREGFRVDVFLTGRQAIA
                     AVKDAAYDVFLLDLMMPHEGGMTVIRHFRVNDPAMLKRSILFTASPDSLIDALSGEVP
                     TVVQKPFEPKQLIDAVRRMSQQ"
     gene            complement(2490..2564)
                     /locus_tag="VNN25_19035"
     tRNA            complement(2490..2564)
                     /locus_tag="VNN25_19035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2530..2532),aa:Val,seq:tac)
     gene            complement(2730..3494)
                     /locus_tag="VNN25_19040"
     CDS             complement(2730..3494)
                     /locus_tag="VNN25_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93685.1"
                     /translation="MRTLASLVLLLTATTASADLFGDCSVSEKRNVAAPTAGITRIVI
                     IGRAGWLHINGRRGAVQVTANGTACASSRDRLADTKLVLTRSGSDLRIEAVTPDNEMF
                     GSSKLDFDVVVPDTIPLEVEDGSGDLTIDNVGPAEVTDGSGDMTIRNVSGNLSVHDGS
                     GDMRLENVSGDVRITDGSGGIEVRGAGSVDIGNDGSGSVEIRNVKRDVHIGNKGSGSV
                     DVSDVGGNFRLGNKGGGSVTWQRVAGQVDVPERYRR"
     gene            complement(3571..4020)
                     /locus_tag="VNN25_19045"
     CDS             complement(3571..4020)
                     /locus_tag="VNN25_19045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH93686.1"
                     /translation="MPYLLDTNHCFRLIARDERMFAALNERPLEQLMTSVVVAGELRF
                     GALISERRIENSLAVEDFLGRIDQIAISPAISQIYADLKARILMKLGPKDRFRRRAFD
                     LAKLGFTDNDLWIAASALERNAVVVSEDSDFRRIAELEQLDVENWLS"
     gene            complement(4492..5085)
                     /locus_tag="VNN25_19050"
     CDS             complement(4492..5085)
                     /locus_tag="VNN25_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93687.1"
                     /translation="MKQKLMTMFGFLTLFCTEFGSSATAQCVFSFNRSGAAIPSSSAM
                     MRHNAATPETGAARTTAESRNNPATAEDAPTITGLWDLQFIADGQVVDEGFDQYHSDG
                     TEILNDTPPPSAGNVCLGVWAKTGGLTLKLKHPSWIYDPTNTFPIGTATILERVTLDS
                     SGNSFTGTFTVQLKDLAGNSLGPDLTGQLQATRITPD"
BASE COUNT          942 a         1523 c         1766 g         1073 t
ORIGIN      
        1 atgggttacg taggtcggac tctccagtcc gactgctcgt gttgtcgcac aaacctcggc
       61 cagtcggact ggagagtccg acctacgtct tcaccttgcc accatcagta agtcggcgaa
      121 gtagccgttg cgatccgtcc agcgctgctc gatcgtgaag ccgccggcag cggcgagggc
      181 gcgcagggtg gcggatgcat tctcgaatgg gttacgtagg tcggactctc cagtccgact
      241 gctcgtgttg tcgcacaaac ctcggccagt cggactggag agtccgacct acgtcttcac
      301 cttgccacca tcagtaagtc ggcgaagtag ccgttgcgat ccgcccagcg ctgctcgatc
      361 gtgaatccgc cggcgttggc gagggcgcgc agggtgtcgg atgcattctc gaatgggtta
      421 cgtaggtcgg actctccagt ccgactgctc gtgttgtcgc acaaacctcg gccagtcgga
      481 ctggagagtc cgacctacgt cttcaccttg ccaccatcaa taagtcggcg aagtagccgt
      541 tgcgatccgt ccagagctgc tcaaccgtga agccgccggc agtggcgagg gcgcgcaggg
      601 tcgcgtcgtc gtacttgtgc gagttttcgg tgtggatcga ctcgcccttg gcgaagacga
      661 cgtcgtagcc gtcgatgagt acgcgctggg ctttgcggct gaccaggtgc atctcgatgc
      721 gtccggcagc ttcgttgtag aaggcgcggt gcgcgaacga agcgagatcg aaggtcccgc
      781 cgagctcgcg gttgatgcgg ccgaggaggt tgagattgaa ggctgcagtg acgccgaggg
      841 gatcgtcgta ggcgggttcg aggattgacg cagacttgcg tagatcggcg ccgaggaaga
      901 gggcatcgcc gctagcgagt gcggtgcgca ggttggcgag catcgcaccg gattcggagg
      961 ggtcgaggtt gccgatgctc gatccgagga agaggacgat cgtccgttcg tccgagggcg
     1021 gaagatgggg ctggagcagt gcggccggct cgcggaagtc gccacagact gcatggatcg
     1081 acagctgtgg gtaatcggtc agcagctcgt gggcgctcgc cgtcaagatg ccagcatcga
     1141 catccaccgg aatgtactcg agcgttgcct gacgctccac gattgcgctg atgaggtggc
     1201 gggtcttcgt ggcgcttccg cttcccagtt ctacgagacg cacgctcggt ccgaagctgg
     1261 ctgcaatctc cgcgctccgt tcgcgcagga tctcatcctc ggcgcgggta acgtagtact
     1321 caggcagctc gcagatcgcc gagaaaagag ccgagccgag cgcgtcgtag aaatagtgag
     1381 gtagcaggaa cttcggtgtg gccgtgagtc cgcggcgaac atcggcggcg aagttggaga
     1441 ccggcgcagc ggcggcgttc tggatcgtga accgcccgcg cgtcacaggc ggcgcggcta
     1501 cggcgagcga tggttgcatg ggagcgggat tgtagcggag ggtgtcgatt ttcgggggtc
     1561 gggtgtcgga ggctggagtc ttcgaattga cggggttacg gtttcacgcg gagacgcgga
     1621 gggcgcggga agggttcttt ctccgactcc cgactcccga cccgcgactc ccgttcaccg
     1681 cacaacatat cccgcagccc gcagcgccgc ggacgcatgc tcgagcacgc cgtcgcgcac
     1741 gaggacgtag tcggtgtcgt aggtcgagac gacgaagagg gcgacgccgg catcggcaag
     1801 aacgcgcgtg aatgcggcgg cgacgccggt ctggtcgagc ggaaacgggc cggccagttt
     1861 caagcagcgc catccgcgat cggccacgac atccgggacg tttgccgcgt cacagacgac
     1921 ggacaactca tcgtgcgtgc gggttatgga gagaaacgcg ccgtgtgccc aggacggtat
     1981 tgcagcatcc gcatgcaggc ggacgatggc gtagtggccg gcgacaagcg tgaacgttgg
     2041 actcgatgcc ttcatcggtg ctcattgctg gctcatgcgg cggactgcgt cgatgagctg
     2101 cttcggttcg aatggcttct gtaccacggt tggtacctcg ccggaaagcg catcgatgag
     2161 cgagtcgggc gatgccgtga agaggatcga acgcttgagc atggcggggt cgttcactcg
     2221 aaagtggcgg atgacggtca tcccaccttc gtgcggcatc atcaggtcga ggaggaacac
     2281 gtcgtaggcg gcatccttca ccgcggcgat ggcctgccgg ccggtgagga agacatcgac
     2341 gcggaagccc tcgcgcccga ggatcttggc gacgaggcgc tgcatggagg ggtcgtcttc
     2401 gagaaggaga acacgtttgc gggttttgaa gaacatggcg ttggtgtcag ctcgcgcaag
     2461 gactgcgcca atgccgcgcc gggaggtgat ggtgcggggt gccgggttcg aaccagcgac
     2521 cctctgcgtg taaagcagac gctctaccgc tgagctaacc ccgcgtaggg gcagagatta
     2581 acacgttgtc ggttgtcagt tgtccgtgcg gtgcgctacc gataacgaca atctgtggca
     2641 tggggagtgg ggggtggggt gtgggagatg gagcacgagg agcgcgttaa tgaccgatct
     2701 cccactccac actcccgaca ccccactccc taccgtcggt acctctccgg cacatcgact
     2761 tgccccgcca cgcgttgcca cgttaccgag ccgccacctt tgttgccgag gcggaaattg
     2821 ccgccgacgt cggacacgtc cacgctgcct gagcctttgt tgccgatgtg cacgtcgcgc
     2881 ttgacgttgc ggatctcgac ggaaccgctg ccgtcgttgc cgatgtcgac gctgccggcg
     2941 ccgcgcacct cgatgccgcc ggatccgtcg gtgatgcgga cgtcgccgga tacgttctcg
     3001 agccgcatgt cgcccgatcc atcgtgaacg gacagattgc cgctgacgtt gcggatcgtc
     3061 atgtctccgc tgccgtccgt cacctcggca ggtccgacgt tgtcgatggt caggtcgccc
     3121 gatccgtctt cgacctccag cggaatggtg tcgggaacga cgacgtcgaa gtcgagtttg
     3181 gagctgccga acatctcgtt gtcaggggta acggcttcga tgcgaaggtc ggagccgctg
     3241 cgggtcagga ccagtttggt gtcggcgagg cgatccctgc tcgatgcgca tgccgtgccg
     3301 ttggccgtca cttgcactgc gccgcggcgt ccgttgatat gaagccagcc cgcgcgtccg
     3361 atgatgacga tgcgcgtgat cccggccgtc ggcgcggcca cgttgcgttt ttcgctgacg
     3421 gagcagtcgc cgaagagatc agccgaggcg gtggtggcgg tgaggaggag aacgagcgaa
     3481 gcgagtgtgc gcattggtgt tacgtacggt gttcgatgcg ctgggtttct gggggggttc
     3541 cgggtgggga tcaggccaac tgatgagccc ttacgaaagc cagttctcga catcaagttg
     3601 ttcgagctct gcgatgcgtc gaaaatcgga gtcctcgctg acgacaacgg cgtttcgttc
     3661 gagagcagat gccgcgatcc acagatcatt gtcggtgaaa ccgagcttcg ccagatcaaa
     3721 agcgcgccgc cggaagcgat ctttcggtcc gagcttcatg agaatccgag ccttcagatc
     3781 ggcgtagatc tgagagatgg ctggtgaaat cgcaatttga tcgatccggc cgagaaagtc
     3841 ttccacggcg agagagttct cgatccgccg ctctgagatc aacgcgccaa aacgaagttc
     3901 gccggcgaca acgacggacg tcatgagctg ctccaacggt cgctcgttga gagcggcaaa
     3961 cattcgttca tctcgcgcga tcaggcggaa gcagtgattc gtatcgagca gatacggcat
     4021 caaaagcgtg ctttcgatct cgtttcggtg acgatacgaa caatctcgtc gagatcctcg
     4081 ccgcaccacc ctggtgctga tcgaagtaat gacgcggccg ttgatcgacg cccttcagcc
     4141 tgaagagatc gggacgcgct ggtaaccccg gtgcctgcgc gcccgcgcgg ggactttgcg
     4201 gcgcgctttt tgtctccccg cttgctctgg tctcgctggt tgtccctggt tttcaacatg
     4261 tcctggtcag tttactgcct cacgcccgtt cgttgaagtt gccgggttaa ttgggtagct
     4321 cggtttcgcg gtttcgggac ggggttagtc aatccgctaa tccccgtgtc ccactgcgtc
     4381 atctgcggat aacgcgtccc agcctcaact tcagccagga acgcgagcac ccaggacaac
     4441 acgtcacgac actgacgctt gccctgggtg gctgcctgcc ccttttctga ctcaatcggg
     4501 cgtgattcgc gtggcttgca gctgacccgt gaggtccggt ccgagcgagt ttcctgcgag
     4561 gtctttgagc tgcaccgtga acgtgccagt gaagctgttg ccgctggagt cgagcgtgac
     4621 tcgctcgaga atggtcgccg ttccgatggg gaatgtgttg gtcgggtcgt agatccacga
     4681 cggatgcttg agtttgagcg tgagtccgcc tgtctttgcc cagacgccga ggcagacgtt
     4741 tcccgctgat ggaggcggcg tgtcattcag gatctcagtt ccgtcgctgt ggtactggtc
     4801 gaagccctca tcgacaactt ggccgtcggc gatgaactgc agatcccaga ggccggtgat
     4861 cgtcggagcg tcttcggcgg tggcagggtt gttgcggctc tccgcggttg ttctcgctgc
     4921 acccgtttcc ggcgtggcag cgttgtgcct catcatcgcg gacgagcttg gaatcgcagc
     4981 cccgctgcga ttgaacgaga acacacattg agcggttgcg ctcgatccga attccgtgca
     5041 aaacagagtc aggaagccga acatcgtcat caacttttgt ttcatcgtag tctcctggtt
     5101 ggtgccggtt cgaagaccgg cggtttgtga tgctgtcctt tcaactgcct tcctccatga
     5161 gcgcgacatt gcgtggcaga acgtctcaaa acatttctgc cgcagctccc ttggctgtta
     5221 ttgttactaa cctcattccc ggaaggagcg catggagaga gacgcgcgca gagagatgac
     5281 cgagcctgcc ggcccaaccg acat
//
LOCUS       DATLVW010000656         4516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_110901, whole genome shotgun sequence.
ACCESSION   DATLVW010000656 DATLVW010000000
VERSION     DATLVW010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110901"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VNN25_19055"
     CDS             complement(<1..553)
                     /locus_tag="VNN25_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HXH93688.1"
                     /translation="MHEITAHLFTLPIPLLEKIIRPIIIYAFLVILLRVFGKRELAQL
                     NPFDLVVLLSLSNTVQNAIIGDDNSVSGGMIGAITLLVTNYLVVRYLFRHRRLDQMME
                     GKPSTLIKEGHVCKKEMARELLTTAELQSVIRREGFDGIQDIETCILEPGGAFSIKGK
                     SPSADVKRHDELLHRLDALTAQIA"
     gene            574..975
                     /locus_tag="VNN25_19060"
     CDS             574..975
                     /locus_tag="VNN25_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXH93689.1"
                     /translation="MSLDDPFDQVAEFHRRFGHPVADRPQLLKSPRADGRVAWMREEI
                     DEFVEATDVVDQADAMIDLIYFALGTLVEMGVRPGPLFDIVHGANMAKLWPDGAPRVR
                     EDGKTIKPPTWRDPAEALREEIERQKTSSRS"
     gene            complement(1116..1880)
                     /locus_tag="VNN25_19065"
     CDS             complement(1116..1880)
                     /locus_tag="VNN25_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXH93690.1"
                     /translation="MNDDLRLALSLADAADEITMRHFQSVTLSVRTKNDRTPVSEADE
                     AVERMIRERLERERPDDGILGEEFGARGEAERRWIIDPIDATKNYIRGIPVFATLIAL
                     DERIGVVSAPAMGRRWWASRGEGAFCRDGGASSQARAIHVSAIAEIGDAQLCYDDVPG
                     FEKLDLGGKFLALVRRCARSRGFGDFWSHMLVAEGAAEIAVEPEVAHWDMAAVKVIVE
                     EAGGRFTNLRGDARADGGSGLSTNGLLHDDVLRAFA"
     gene            2037..3530
                     /locus_tag="VNN25_19070"
     CDS             2037..3530
                     /locus_tag="VNN25_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXH93691.1"
                     /translation="MNLIRRSSSIVLVLIFATVARAQTPILHWGGDAEGGAPFVEADP
                     NDPSNLRGFDVEVAGEIAKGLGRRPEFVQVSFSDIDQSVQRGDFDIGMSGVEDTPARR
                     KMLAATIPYFEFHEVLTVRAEDRGKFATLAALRGRRVGTLGGTIAYEKLLAAQMTDGV
                     ICVSYDDDVHPYADLRAGRLDAVLLDNIIAARSMRRVPGLYTEPKPVATSHYIGVLAT
                     TNTALRDRINGILRARMADGTLERIYRKWKIWDDYQPAFFASVLNAEKAVQQHERKTA
                     EGGATQDLGIVLRYIPALLRASLITIVLSCAAMALAVLVGVFIASGRVYGSAPVRAVL
                     TMYVEVVRGTPVLLQLFVLYYGLSSVIRLPAFLAALLGLGLNYAAYESEIYRGALEAV
                     PRGQLEAARTLGLTESQVLRLVRGPQAFRIALAPMTNDFVALLKDSSLVSVITVIELT
                     KQTSIFAANIGSWVIPGILCAALYLVLSLPLSRLAGRLEMRWKAATE"
     gene            3527..4279
                     /locus_tag="VNN25_19075"
     CDS             3527..4279
                     /locus_tag="VNN25_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH93692.1"
                     /translation="MSGVLSIANLRLRRGEREILRGVNADVRAGELVALMGLSGGGKT
                     TVLRCVAALETFEDGVIDVGGVVLRPGPAPSRNTIRELRTRVGMVFQLHYLFEHLSAI
                     GNVTLAPMQVAHVPRVEAERRATELLEALGVAHRRNALPRELSGGEAQRVAIARAMAM
                     EPPLLLMDEPTASLDPARRGELGETLLQLTTTGRTLVVTTHDDDFARDFATRVIILAE
                     GEVVEEGNPREVLTNPQHAATKRLLQSARSEK"
     gene            4303..4482
                     /locus_tag="VNN25_19080"
     CDS             4303..4482
                     /locus_tag="VNN25_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93693.1"
                     /translation="MKQPTQRPLTEDEIDEIVISQAYDDDAWEEEFEVTPISRPRARD
                     ANPPVKHPAGRPQQR"
BASE COUNT          792 a         1388 c         1523 g          813 t
ORIGIN      
        1 cggcaatctg cgcggtcagt gcgtcgaggc ggtgcagcag ttcgtcgtgg cgcttcacat
       61 ccgccgacgg tgacttcccc ttgatactga aagcgccgcc cggctcgagg atgcatgtct
      121 cgatgtcttg aatgccgtcg aagccctcgc ggcgaatgac gctttgcagc tcggccgtcg
      181 tgagcagctc cctggccatc tctttcttgc agacgtggcc ctccttgatg agtgtggacg
      241 gtttgccttc catcatctgg tcgagccggc gatggcggaa gagatagcga accacgaggt
      301 agttggtcac cagcagcgtg atggcgccga tcatcccgcc gctgacggag ttgtcgtcgc
      361 cgatgatggc gttctgcact gtgttcgaca acgacagcag cacgacgagg tcgaacgggt
      421 tgagctgcgc cagctcgcgc ttgccgaaga cgcgcagcag gatcaccagg aaggcataga
      481 tgatgatcgg ccggatgatt ttctcgagca gcgggatcgg aagggtgaag agatgtgcag
      541 tgatttcgtg catggaacta tgatgcaccc tctatgagtc tggacgatcc tttcgatcag
      601 gtcgcggagt tccaccgccg tttcggacac cctgtggcag accgcccgca gctcctgaaa
      661 tcgccgcgcg cagacggccg cgtggcctgg atgcgcgagg agatcgacga gtttgtcgaa
      721 gccaccgacg tcgtcgatca ggccgacgcg atgatcgacc tgatctactt cgccctcggc
      781 acactcgtcg agatgggcgt gcgtccggga ccgttattcg acatcgtgca cggcgcgaac
      841 atggccaagc tctggccgga cggcgcgcca cgcgttcgcg aggacggcaa gacgatcaag
      901 cccccgacgt ggcgcgatcc cgccgaggcg ttgcgcgagg agatcgagcg gcagaagacc
      961 tcgtcgcgtt cttgattgta ggaaagcggt ggctgcgcca cgcgcagtcc aaagcgcttc
     1021 gcgcaaaacg ttgggcagaa tttcgcgcga agcgctttgg agtgcggtgg cgcagccacc
     1081 gctttcgagt gtttgcgaat tgtcccggag tgaacttacg cgaaggcgcg caacacgtcg
     1141 tcgtgcaaaa gcccgttcgt cgagaggccg ctgccgccat ccgcgcgcgc gtcgccgcga
     1201 agattggtga accgtccgcc ggcttcttct acgatcacct ttaccgcggc catatcccaa
     1261 tgcgcgacct ccggttcgac ggcgatctcg gcagccccct cggctacgag catgtgcgac
     1321 cagaaatccc caaatcctcg gctacgcgcg cagcggcgca cgagcgcgag aaacttgccg
     1381 ccaagatcaa gcttctcgaa gccgggaacg tcgtcgtagc agagctgcgc gtcgccgatc
     1441 tcagcgatcg ctgatacgtg gatcgcgcga gcctggcttg atgcgccgcc gtcgcggcag
     1501 aacgcaccct cgccgcgcga cgcccaccag cgcctgccca tggccggcgc ggagacaacg
     1561 ccgatccgct cgtcgagcgc gatcaacgtg gcgaacaccg gaatcccgcg gatgtaattc
     1621 ttcgtggcgt cgatcggatc gatgatccag cgccgctccg cctcgccccg cgctccgaac
     1681 tcttcgccga ggatgccatc gtccggccgc tcgcgctcga ggcgctcacg aatcatcctc
     1741 tccaccgcct catccgcctc ggacaccggc gtccgatcgt tcttcgtcct caccgaaagc
     1801 gtgaccgact gaaagtgccg catggtgatc tcgtcggctg catcggcgag agagagggca
     1861 aggcggagat cgtcgttcac gcgtgcgatt gtagcgttgg agtgcgggcg tctcgccagg
     1921 cgtctcgtcc gcggccaccg ggcgtccccg cccggtggtt tgcgcaaaga ggaaaaatca
     1981 tccaagcgaa aaccgttgac aaagccggcg ctttgaacga tgaacgtcgc cacaaaatga
     2041 accttattcg ccgatcgtca tccatcgttc tcgtgctcat cttcgcaacc gtggcccggg
     2101 cgcagacacc tatcctgcac tggggtgggg atgccgaggg gggagcgccg tttgtggaag
     2161 cggatccgaa tgacccttcg aatctgcgcg ggttcgacgt cgaggtggca ggggagatcg
     2221 ccaagggact ggggcggcgg ccggagttcg tgcaggtgtc gttcagcgac atcgatcagt
     2281 ccgtgcagcg cggtgacttc gacatcggga tgagcggcgt tgaggacacg ccggcgcggc
     2341 ggaagatgct ggcggcgact attccgtact tcgagtttca tgaagtgctg accgtgcgcg
     2401 ccgaggatcg ggggaagttc gcgacactgg cggcgctgcg cggacggcgc gtcggcacgc
     2461 tcggcggcac gattgcgtac gagaagctgc tggcggcgca gatgaccgac ggcgtgatct
     2521 gcgtctcgta cgatgacgac gttcatccgt acgccgatct tcgcgccgga cgtctcgacg
     2581 cggtgctgct cgacaacatc atcgcggcgc ggtcgatgcg gcgcgtgccc ggtctttaca
     2641 cggaaccgaa gccggtggcg acgagtcact acatcggcgt gctggcaacc accaacacgg
     2701 cgttgcgcga tcggatcaac ggcatcctgc gcgcgcggat ggccgacggc acgctggagc
     2761 ggatttatcg caagtggaag atctgggacg actatcagcc ggcgtttttc gcgagcgtgc
     2821 tgaacgcgga gaaggcggtt cagcaacacg agaggaagac ggccgagggc ggcgcgacgc
     2881 aggatctggg gatcgtgttg cgatacatcc cagcgctgct gcgggcttcg ctgatcacga
     2941 tcgtgttgtc gtgcgcggcg atggcgttgg cggttctcgt cggcgtgttc atcgccagcg
     3001 gccgtgttta tggcagcgcg ccggtgcgcg cggtcctgac gatgtacgtc gaggtcgtgc
     3061 gcggaacgcc ggtcctgctg cagctcttcg ttctttacta cggtctctcg tcggtgatcc
     3121 ggctcccggc atttcttgcc gcgctcctcg ggcttggact gaactacgcg gcgtatgaaa
     3181 gcgagattta ccgcggcgcg ctggaggctg tgccgcgcgg tcagctcgaa gcggcgcgga
     3241 cattgggttt gacggagtcg caagtcttgc ggctcgttcg cgggccgcag gcatttcgca
     3301 tcgcgctggc gccgatgacg aatgacttcg tggcgctgct caaagattca tcgctcgtgt
     3361 cggtcatcac cgtaatcgag ttgacgaagc agacctcgat cttcgcggcg aacatcggaa
     3421 gctgggtgat tcccggcatc ctctgcgcgg cactgtatct cgtgctgtca ctgccgctgt
     3481 cgcgcctggc gggacgcctg gagatgcgct ggaaggcggc gacggaatga gcggcgttct
     3541 ttcgatcgcg aatctccgcc tgcgccgcgg ggagcgcgag atcctgcgcg gcgtcaacgc
     3601 agacgttcgc gccggcgagc tcgtcgcgct gatgggcctg tccggcggcg gcaagacgac
     3661 ggtgttgcgc tgtgttgcgg cactggagac gttcgaggat ggtgtgatcg acgtcggggg
     3721 tgtggtgctc cgcccgggac cggcgccttc gcgaaacacg atccgcgagc tgcgcacgag
     3781 ggttgggatg gtgttccagc tccactacct cttcgagcat ttgtcggcca ttgggaacgt
     3841 cacgctggcg ccgatgcagg tggcgcatgt tccgcgcgtc gaggccgagc gccgcgcgac
     3901 ggagctgctg gaagcgctcg gcgtcgcgca ccggcgcaac gcactgccac gcgagctatc
     3961 gggaggcgaa gcacagcgcg tggcgatcgc gcgcgcaatg gcgatggagc cgccgctcct
     4021 actgatggac gagccgacgg cgtcgctcga tccggcccgc cgcggcgagc tgggagagac
     4081 gctcctgcaa ctgacgacca ccggccggac gctggtcgtc acgacgcacg acgacgattt
     4141 cgcccgcgat ttcgccacgc gcgtgatcat tctggccgag ggagaagtgg tcgaggaagg
     4201 aaacccgcgc gaggtgctga cgaacccgca gcacgcggcg acgaagcggc tgttgcagtc
     4261 ggcacggagc gagaagtagc gtgaggccgt tataattcgg ggatgaagca accgacgcaa
     4321 cggccgctga ctgaggacga gatcgacgag atcgtaatca gtcaagctta tgacgacgac
     4381 gcttgggaag aggaattcga ggtgacgccg atctcgcgac ctcgtgcccg cgacgcgaat
     4441 cctccagtga agcatccggc gggccgtccc cagcagcgat aactaccacc tcgtttgtga
     4501 ccccgatcac aagcat
//
LOCUS       DATLVW010000657         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_26213, whole genome shotgun sequence.
ACCESSION   DATLVW010000657 DATLVW010000000
VERSION     DATLVW010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26213"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VNN25_19085"
     CDS             complement(<1..1115)
                     /locus_tag="VNN25_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93694.1"
                     /translation="MTEPVFAQPVEPLTTGQRNLIVALAIVVAATRFLAVSHSMWDWD
                     EALFASALQHYDVAQHHPHPPGFPLFFALAKLVRLVGHNDFHALRAVSVFASLFVFPA
                     FFALARSLRFRFRTCAVGAALFCFLPNVWYWGGTGFTDELALVTALVSAALLLRDDQR
                     HRTYIGGCLLFAATMLVRAQNVLLLWPWIEASWRRMRAKRTWEVVTGTALIVVLVVTG
                     YGIAAKLTGFAEFVSVTKEHQHYIATVDGSLNPRRIPMANLLQDFLFDPFESRAASTA
                     LFGFALLSFARPRRAHAVILLTFLPNFFLAWFLLSPTGISRLSLGYIAFDVLLAADGM
                     DVAAAFLASLLRAIPRERVAAALQMLFAAVVIGRYIF"
     gene            complement(1112..>2046)
                     /locus_tag="VNN25_19090"
     CDS             complement(1112..>2046)
                     /locus_tag="VNN25_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93695.1"
                     /translation="TFVPNFLLAWFFLSATGISRLSLGYIALNALLAADGMDVIASFV
                     TLRWRALRRESLATALQMFLAAIVIGRYIFWVWPALEEVRTKDSPPVLAMRWIREHVP
                     GGTTIYLNGALEPFPEYFLPDYRIVSVDADFDPSRVVLQPGAFVVSDAPSRLADAVNF
                     QRPHRRLWALFNQRYFETSVAPAAGWIKFGDGWYNEEDNGQGVWWRWMRSESHTLLGP
                     LGHPAQLGVTLGYPDELEPAPKVVIALDGNVIDRFVVPRGAAERSYAVSSRSGASDEL
                     VISVDRTVNPLRAHRGPDSRDLGLRLLSLTWKAR"
BASE COUNT          479 a          640 c          646 g          281 t
ORIGIN      
        1 cagaagatat agcgcccgat gacgaccgcg gcgaagagca tctgcaacgc cgccgcaaca
       61 cgttcgcgcg gaatggcgcg aaggagcgac gccagaaacg ccgccgccac atccatccca
      121 tcggctgcca gaagcacgtc aaacgcgatg tagccgagcg ataggcggct gataccggtc
      181 gggctcagaa ggaaccaggc cagaaagaag ttcggcagga acgtgagcaa aatgacggca
      241 tgagcccgcc tcgggcgcgc gaacgacaga agcgcgaatc cgaacaacgc cgtcgacgcg
      301 gcgcgtgact cgaacggatc gaacaggaag tcctgcagta gattcgccat cggaatccgc
      361 cgcgggttga gcgacccatc gacggtggcg atgtagtgct ggtgctcttt cgtgacggag
      421 acgaattccg cgaatccggt cagctttgcg gcaatgccgt agccggtcac gacgaggacg
      481 acgatgagcg ccgtgccggt gaccacttcc cacgtgcgct tggcgcgcat gcgccgccac
      541 gacgcctcga tccatggcca caacagcaga acgttctgcg cgcggacgag catcgtcgca
      601 gcgaacaaga gacatccgcc gatgtacgta cggtggcgtt gatcgtcgcg aagcagcagc
      661 gcggcactga caagcgcggt aacgagtgcc agttcgtcgg tgaagcccgt ccctccccag
      721 taccagacgt tgggcagaaa acaaaagaga gcggcaccca cagcgcaggt ccggaaacgg
      781 aaccgcaacg agcgcgcaag agcgaaaaac gcggggaaca cgaacagcga cgcaaacacg
      841 ctcacggccc gcagagcatg aaaatcgttg tgaccgacga gacggacgag cttcgccagc
      901 gcaaagaaca gcggaaaacc cggcgggtgc ggatggtgct gggcgacgtc gtaatgctgg
      961 agcgcagacg cgaagagagc ctcgtcccag tcccacatcg aatgagaaac cgccaggaat
     1021 ctcgtcgcag caacgacgat tgcaagcgcg acgatcagat tgcgttgtcc cgtcgtgagc
     1081 ggctcaacgg gctgcgcgaa aaccggctcc gtcatcgcgc tttccatgtg agcgacagaa
     1141 ggcgcagacc caggtctcgg gaatcggggc cgcgatgcgc gcggagcgga ttgaccgttc
     1201 gatcgacgga aatcaccagc tcatcggaag cgccggagcg cgacgaaacc gcgtacgaac
     1261 gctcggcggc tccccgcggg accacgaagc ggtcgatcac gtttccgtcg agcgcgatca
     1321 ccaccttcgg cgccggctcg agctcgtccg gatacccgag agtcactccc aactgcgcag
     1381 ggtgaccgag cggtccaagc agcgtgtgac tctcgctgcg catccatcgc caccaaacac
     1441 cctggccgtt gtcttcttcg ttgtaccagc catcgccgaa cttgatccag ccggcggccg
     1501 gggcgaccga tgtctcgaag tagcgctgat tgaagagcgc ccacagccgg cggtgcggac
     1561 gctggaaatt gaccgcgtcg gctaaccggc tgggcgcatc ggacaccaca aacgcgcccg
     1621 gctgcagcac tacccgcgat ggatcgaaat ccgcgtcgac ggaaacgatt ctgtagtccg
     1681 gaagaaagta ttcaggaaat ggttcgagag ctccgttcag gtagatcgtc gtgccgccag
     1741 gaacgtgttc gcggatccat cgcatcgcca gcaccggcgg cgagtccttc gtgcggactt
     1801 cttccagcgc cggccagacc cagaagatgt agcgcccgat gacgatcgcc gccaaaaaca
     1861 tttgcagtgc cgtcgccagc gactcacgcc gaagcgcacg ccagcgcaag gtcacaaaac
     1921 tggcgatgac gtccatcccg tcggcggcaa ggagcgcatt gagagcgatg tatccgaggg
     1981 agaggcgact gatgccggtc gcgctaagga aaaaccacgc cagcagaaag ttcggcacga
     2041 acgtca
//
LOCUS       DATLVW010000658        13629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_606438, whole genome shotgun sequence.
ACCESSION   DATLVW010000658 DATLVW010000000
VERSION     DATLVW010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606438"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1061
                     /locus_tag="VNN25_19095"
     CDS             408..1061
                     /locus_tag="VNN25_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93696.1"
                     /translation="MPSRNSLLWGTRSCCLIGSMTAQETYRAAGADIPAGELEAYGNH
                     LFAKGELDRGVIAYSLAGVDVPPEQLIICGEWFNRMRKFDEAVRAFKFAGASKHLVEL
                     GDRLLADGETVRLYIALKAYLAAGPDAPNDKLIALGDKFSDRDLGDPAIDAYVAAQAI
                     DRLDAFSSRLSSTGWSVHAKRAREEMRRISHRQQDEQRGAASNSSSPSAGDTSTSPL"
     gene            1719..2468
                     /locus_tag="VNN25_19100"
     CDS             1719..2468
                     /locus_tag="VNN25_19100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXH93697.1"
                     /translation="MRIVEGWIAWLRLQEVSRVAINTYWRGLRLLLRWMQEQDGTLNP
                     LVYLQTPRIGRILPRYLTKTSAEDMLTWLRNFPWGSPLERTRNLSIISLMLMAGLRRS
                     EVVKLHFADVNTAEGLIRVQGGKGRYGGKDRTCYMPPQLVEILDAYIGERSRAKRSHP
                     EFITDIRANRGIGAEPVRRLCERISRETGIQVRPHALRHTYATLLRMSGVPDRVAMDL
                     LGHSSYAMLQRYSHVFTGEHQKEASKLHFDL"
     gene            2480..4012
                     /locus_tag="VNN25_19105"
     CDS             2480..4012
                     /locus_tag="VNN25_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93698.1"
                     /translation="MATVRGSFRALHRLIVQKFQMLCTALRHPPLLHAAVLQTAAHHG
                     ANQLPREVPVLSTPVRVEAQTPIEDFGVTTFGFEVPEEDDPAELLRDAARRGEQERAT
                     IDVLQGETLTAAQAPVMESVAAQRVSVEPRGTPAAMADESEYATPIDNVQGHRDGEEP
                     LSSSVFPLPSAEAGDGSSAGPFEAVEGTPLRVPRTPKPVPTPLHAVRDMDAIKLIVRL
                     RLLSYRQLRSWLFPGHPSMVGRSMQRLAKRDWLALHMDYLTLGGHPIYAFPTAKAMRW
                     AREQFLKMVAGTSHEKLVQTMLPNEVKSLSIPQPRSAPAYLAHQELSNDAAFALEHDP
                     KLRIPWLSTWPRPFPHTYSAMPLPQPDLVLVAEVKGTPYLFFGELDHNSNESLAHFQN
                     RKAHRARVFDHSNGLHVLTGFTTFRMLVIIADAKDPLGRMKKLIAATKRAGASHLLAF
                     TLMPWLLLDPSGLIWFADGMTPTHGSLAAGKHRGLKRFEQLARKPETVEEILARERRR
                     RV"
     gene            4421..4765
                     /locus_tag="VNN25_19110"
     CDS             4421..4765
                     /locus_tag="VNN25_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93699.1"
                     /translation="MERIRGRCAVLPARNPDLPILHVVELEQPCFAPSQPVPVDEIEE
                     QEVACLSLRNRVEEALNLLSREVLDRFLLAASFIRHSVMSLRTGSDSDTFPHLRSVGR
                     QAEEEDASQPAS"
     gene            4925..5380
                     /locus_tag="VNN25_19115"
     CDS             4925..5380
                     /locus_tag="VNN25_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93700.1"
                     /translation="MKQLRIRELFLQRAESYSLSEAARIVGVSTRALRREAEDDQREA
                     YLSNGTWCFTWRQVAFIAMRRWTLAEIHEALGAEAETVLPPLLAMDVLTVRLPAFLIR
                     AMMTAAEQDAIPLDEWLRLELIDYAGTVVDEMEAIHPGFRRAYLFPGQK"
     gene            complement(5440..7647)
                     /locus_tag="VNN25_19120"
     CDS             complement(5440..7647)
                     /locus_tag="VNN25_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stress protein"
                     /protein_id="HXH93701.1"
                     /translation="MSEPSFGARIRECSVEDVLALERGYAPAAVHAQLDGILSGIIRS
                     SLDGTRVAVTREAVKPYDTVELLTLAEADRAVIAKAFDLAAQQGRGAWFLPDEAHLNV
                     GWANMPHHFGRYERFATGIARDESGKVRFQASPEALYFWSVLNPLFDLLYRPFQLRGA
                     TPPSQDRDEQRHAWEEIDRTYDALGLPVAEGLAAMRFGSGWSKLRRAEQLVAKRALLT
                     ALRASAPFDIGARYRVWVTQELLAKYYAKTSRGVPTMRKVLTKPLQRTLAGFFGGDWL
                     SFLAYIGETPSSDEQISTALPEPRLYVGAASRAKEVAARHGVPAEEVERMLAAFWTSS
                     TTESPVHQRIRVLREYWEHFEDAHARQAPGMQPLWGFADDDSAVRLRGLDDSQTGPPW
                     YHPGQYRAVLPASLLADIARLWSGVFMPSAPDRIVTAIGPYGGMLAAFGPALRFWDGV
                     GLTAWFVSEGPMSRTDMAGLEKYHERDIGALNVLGCPIDAKMFAELVAAENTLGSPEP
                     ITDPKHSSKINAGGITISMTMNVGSRREGFVRLRDIVTNYRRAWATKFLDAYLRARWE
                     SEIRLAAREYNRHIEVKRKAPTAKQFAKFAEAPANHWFGGDVSLLYAAFGEKSPVATQ
                     RARLLPSDTQAFALRVFEAIGGTKTSWSAFAYETDGPERERRQAEWNAHWKRKDLAEA
                     SIRYVQLREALGRQPSITEFGRSKFEYLGIALHDDPEEAWKAYTRAVDNLLAL"
     gene            complement(7640..9685)
                     /locus_tag="VNN25_19125"
     CDS             complement(7640..9685)
                     /locus_tag="VNN25_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXH93702.1"
                     /translation="MALDLSAASYLEAAFVRWVLAPAVTADIRPHVVAQADVAVHSHT
                     YRIDYEIVGETQRIAIELDGFEFHSNRSAFTYDRMRQNDLAATSRLVVRFSYDAVRLD
                     TARCVAQLQSVLQLDPLLRQFIVQNPIIERPDMDPNPLRALESTQLHRPPMTYFDAVR
                     DKISQRTLRQCQTEAFSALGNYFRSGGTNAACVMSVGAGKTALGVASILAFTQRRALI
                     VTPGSVIRGTFDKALDHEAVGNALYGFPNGPLIPGCPPPKVLTLDREEGAIRSITHAM
                     LTSADVILTNFHSLGTGDDPDDLLAKLAPDDIDMIVVDEAHIAASESYQRLFRHFAGA
                     RTFLMSACFQRLDGKPIDADVVYRYRLIDSIADGNAKNLRVQRFAPDEAQSIYEMVWP
                     DGSREEIRGREAILNLLKDEKKLATITAKSHEPIRQVMRAVRTALDQQAALLTPVKPR
                     VLFSAIGERHAEQIARIAEELGIPCAHVHHSMSPSRIKAIRARFEQESGDLQGIVQLK
                     MLGQGYDFPPITVVVPMRPYGSFSEFYQFVGRGIRVLSHPALKGRVGPPQQFLDIIYH
                     AELGLDVHIETIYRENDMDPITEHVLPPNWNSSTTEREPLPIAGGTNTAERPEAFVLF
                     QPGAIEQRIVHDDVRVEQRRDEREREALAQRYASYAQGTSSPVTFEQFEDIMRNLRE"
     gene            complement(9690..10313)
                     /locus_tag="VNN25_19130"
     CDS             complement(9690..10313)
                     /locus_tag="VNN25_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93703.1"
                     /translation="MDDIHWYERLGNVPGITYEGHPDVDRRVLPQHNAKPSVESFVFD
                     GRTQRSLSWKTESGSTSRSPAAGWSGGDDPRKTSAPQLLRRLYEAIELPGTISDYHFA
                     MLGVYQALWAHRKREPEILPELEKILLLDISLVEAHPKSITFDTQGEEFVPHVPAFEY
                     LVALYEREGFLHEALDIAKRAVKAGQDSSDEERLTERIAVLDAEDES"
     gene            complement(10394..>13629)
                     /gene="avs2"
                     /locus_tag="VNN25_19135"
     CDS             complement(10394..>13629)
                     /gene="avs2"
                     /locus_tag="VNN25_19135"
                     /inference="COORDINATES: protein motif:HMM:NF041813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AVAST type 2 anti-phage system protein Avs2"
                     /protein_id="HXH93704.1"
                     /translation="TSYTVDVVPESLGEHLTRETHPGFAAHEYQATRTFFEWFGIKRP
                     SIPLLNPEFQNPLFLKLFCKGIKDAGLTEMPKGLHGVTQMFDFLLDAVNQKLSGPDQL
                     DFDPKKHLVHEALRSVAQQLASSASHFLTRNEAQATVDAHLPNRTYNNSLFARLLSEG
                     LLTEERVWRPEGLREGIRFAYERLQDHQIASYLIDMHVAEDDIATALEPEQPLGARVR
                     EYTREWTFGLIEALSIQVPERFGVELADVMPDAADSSPIRRAFIRSLLWRRPQAIGEP
                     AKKYLRAGLTQAEFDELLDVVLTVAANPDHPFNADYLHARLMRDPMPERDAWWSIYLT
                     EQYGEHTAVDRLVEWAWSSEDKSHIDDESVRLTATALMWFLTTSHRPLRDRATKALVR
                     LLTPRLPVLESLLTAFRDVDDPYVRERLYGIAYGCVLRSSDGNAIGGIAQHVFEDVFK
                     DGTPTPHILLRDYARGVVERAAALGVDGGLDLGRVRPPYKSDWPENLPSAEELKKKYD
                     ERPEGSGDEGYAQSTIYFSVAGGGDFDRYVIGTNSHSFNWSAIRLDESVPPTKRERHD
                     AFAESLTDEQREALERFETASHGAFFKLLTASYQQQPEEETETSNPEGADEPRPPRAF
                     RRVSDEEREHIEQELEREIADTENAFRKMLGPEELERFETEALPYIEHSGKEEEHLFD
                     LGIAQRFVLNRVFELGWTIERFGFFDRNLRHGSVGREANKPERIGKKYQWLAWHEFLA
                     RVADHFQYEDHMAREVRKYDGPWQDFDRDLDPSLLIAATASERSTSAAAWWSPLMPYG
                     DWRSIGSDAAWVAESSDLPPVEPLIAAQRPDGREFLVLETYREWTEPAPPGVERYDIE
                     RREIWYMVKSYVVRREHADAFYQWGSQQNFYGRWMPESHEMTRVYFGELFWAPAFHYH
                     STRHTGRRAWVGGDEEDSRLPAPVLVSTDAYMCEHSTFDCSLDETVNIHLPAAELVEW
                     MGLTWRGREGYFVDQHDAEVAFDPSVFEAGPGAVVVDRARLSAMLEKQGLAMIWTVLG
                     EKRVLAPLGEHRTAPYIISGCYRLRDGQLEGAVRIVAEDAERAGPSN"
BASE COUNT         2653 a         4170 c         4082 g         2724 t
ORIGIN      
        1 gctgcgggcg cggaatcaga actcgtggag attggtaccg ccgcccttgc tgcgggtgat
       61 tccgggaatg cgcggaacgc cttcgttgca gcggagcgca tcctgatcta gcaggaacac
      121 gcaagctaac aactcttctc agaatcccag ctctggactc ctagaattct tcttccatca
      181 ttccactcca atgcccagca ccaagaacga tctccacaca tcggctgaag ctcacggcgg
      241 tttggatcag ccggtttcgc ccgagactgg acgggacgac gcgccctccg gtgatgtcgt
      301 gatcgatgag acgcaccaag cgatcgccgc cgtcgttgcc gcggacacaa tccaagaaca
      361 gttcgatctt ccagggagca gcccgagaca gcgatacgcg gaagcgtatg cccagcagaa
      421 actcattgct ctggggcacg cgttcttgct gtctgatcgg ctcgatgacg gcgcaagaga
      481 cctatcgagc tgcgggtgcg gacattcctg cgggagagct tgaagcgtat ggcaaccacc
      541 tgtttgcgaa gggagaactc gacagagggg tgatcgcgta ttccttggcg ggggtggatg
      601 tcccacctga gcagctcatt atttgcggag agtggtttaa ccgcatgcga aagtttgacg
      661 aagccgtgag ggctttcaag tttgcaggag catccaagca tctggtcgaa ctcggagatc
      721 gcctgctcgc agatggagag acggtgagac tgtacattgc cttgaaggcg tacctcgccg
      781 ccggtccaga cgcgccgaat gataagttaa tcgcacttgg cgacaaattt agtgatagag
      841 accttggtga tcctgccata gacgcctacg tggctgcaca ggctatcgac aggcttgatg
      901 cgttcagcag tcgcttgagc tccacgggct ggtcagtgca cgctaagaga gcacgtgagg
      961 aaatgaggcg cattagccat aggcagcaag acgaacagcg aggagcagcg agcaattcat
     1021 cgagccccag cgctggcgac acttctactt ctccgcttta gaggagggta gttcgcgcgt
     1081 ggtctcgatc gggtccgcca tcgtaatccg cttgtccttt cgctaagcag ggccacagag
     1141 aggcccgcag atatcgctgg catagtgcca gtcgcctcgg cgacttttcg acatgcacaa
     1201 aaggaaatca gctggcagtg tgccagaacg caatctctct actcggcaca atcgttgtag
     1261 cacaggagaa tgctggatac gcgacggtct ttcgcaccgc tagacattcc tgtatagtgt
     1321 ggtcccttaa ttaactcctc cttcctccgt atctctttac cccaacgcct atgcagcagc
     1381 cggtctccgg aactggccca ctctccatcc gcatccccgt gcgtgtggtg cagcggccct
     1441 ccctccccaa gacacccaca caaacacccg ccctcgttcc gattccgcgc tccggccgtc
     1501 agacttacgc tctgccattc acccctctcc cgctccagtt cccctcgctc gaacatctcg
     1561 atcggcgcgt cacagagtgt cttgtgcatg cgcaggctgt gaacggccta acagacgcga
     1621 cgtgcgcatg gatacgaaag tcctatgctt cgtttcggcg gtacctcaag gccacgcaga
     1681 ctgagggtct attcctcggc ggcgatattc gcaggcaggt gcgtatcgtc gaaggctgga
     1741 ttgcctggct gcgtctacaa gaggtctcac gcgtcgccat taatacctac tggaggggac
     1801 tgcggctgct cttgcgttgg atgcaggagc aggacggcac cctcaacccg ctcgtctatc
     1861 tgcaaacgcc gcgcataggg cgcatcctcc cgcgctacct cacaaaaacg agcgcagaag
     1921 acatgctcac atggctgcgt aatttcccat ggggaagccc gcttgagcgc acgcgcaatc
     1981 tctcgatcat ctccttgatg ctgatggcgg gtctgcgccg cagcgaagtg gtgaaactgc
     2041 acttcgcgga cgtgaacacc gctgaaggac tgatccgcgt gcagggcggc aaggggcgct
     2101 acggcggcaa ggatcggacc tgttacatgc cgccgcagtt ggtggagatc cttgacgcgt
     2161 acataggcga gcgaagccgg gcgaagagat cccacccgga gttcatcacg gatattcgcg
     2221 cgaatcgcgg catcggtgcc gagccggtgc gccgtctctg cgagcggatt tcccgtgaga
     2281 ccggcattca ggtgcggccg catgcattgc gccataccta cgccacactg ctgcgcatga
     2341 gtggcgttcc tgaccgcgtt gcgatggatc tcctcgggca ctccagctac gcgatgctcc
     2401 agcggtactc gcacgtgttc acaggggagc atcaaaagga agcgtcgaag ctccatttcg
     2461 atctctaacc cgcacgccca tggctactgt ccgcggttcg ttccgcgcac tccatcgcct
     2521 gatcgtgcag aagttccaga tgctctgcac ggcactccgg cacccgcctc tcttacacgc
     2581 tgcggtactc cagacggcag cgcatcacgg agcgaaccaa ttgcctcgcg aagtgccggt
     2641 cctgagcaca cccgtccgcg tcgaagctca aacgccgatt gaggatttcg gggtcacaac
     2701 gttcgggttt gaggttcctg aagaggatga cccggcagag ctgcttcggg atgcggcgcg
     2761 ccgtggcgaa caggagcgcg caaccattga tgtgctccaa ggcgagacgc ttactgctgc
     2821 gcaagcacct gttatggaga gcgttgcagc gcagcgtgtt tcggttgagc cgcgcggcac
     2881 tcccgcagcg atggccgacg agagcgaata cgcgaccccc atagataatg ttcaggggca
     2941 tcgcgatggc gaagaaccgc tttcttcctc ggtttttccg ctgccgagtg ccgaggccgg
     3001 agacggcagc tcggcaggac ccttcgaggc agtggaaggc acccctctcc gcgtgccgcg
     3061 aacccccaag ccggtgccga ctcccctcca tgccgttcgt gacatggacg cgatcaaact
     3121 catagtccgg ctccggctcc tctcctaccg ccagcttcgc tcctggctct ttcctggcca
     3181 cccgtcgatg gtcggtcgaa gcatgcagag actcgctaag cgcgactggc tggccctgca
     3241 catggattac ctcacgctcg gtggtcaccc catttacgcc tttccgacgg ccaaggccat
     3301 gcgctgggcg cgcgaacaat tcctgaagat ggtcgcaggg acttcgcacg agaagctcgt
     3361 gcaaaccatg ctgccgaatg aggtgaagag tctctcgatc ccgcagccgc gcagcgcgcc
     3421 ggcctacctc gcccatcaag agctgagcaa tgatgctgcc ttcgcgcttg aacacgatcc
     3481 caaactccgc atcccatggc tctccacctg gccgcggcct ttcccgcata cgtacagcgc
     3541 gatgccgctg ccccagcctg atctcgtcct cgttgcggag gtaaagggca caccgtatct
     3601 cttcttcggc gagctcgatc acaacagcaa cgaatcgctc gcgcactttc agaaccgcaa
     3661 ggcccaccgg gcgcgcgtct ttgaccattc gaatgggctc catgtgctga ccggcttcac
     3721 gacgttccgg atgctcgtca tcatcgccga tgccaaagac ccgctcgggc gcatgaagaa
     3781 gctcatcgca gcgacgaagc gcgcgggtgc gagccacctg ctcgccttca cgctgatgcc
     3841 atggctcctt ctcgatccgt caggtctgat ctggtttgct gatggaatga cgccgacgca
     3901 cggatcattg gctgcgggga aacatcgtgg actcaagaga ttcgagcagc ttgcacgcaa
     3961 gccagaaacg gtcgaggaga tccttgcgag ggagcgaagg cggcgggtct gacttgacga
     4021 aaatgcggaa aagcgttcgt tcgcgattgt cactcgccgc cgttcgtccg cgcgatttcg
     4081 cgtgagcgca gggtcgttcg gtagttcgcc tcgcggcgct tgttggtgat gcgaaagtac
     4141 acgagtgttg atgagatgtc gcggtgtgtc cgagccagtc ttgtacatcc gcgatgtccc
     4201 aattcgcatt ggcgagatgg acgccgatcg agtgccgaag gacatgaacg tggcggcgat
     4261 cggccggaag gtctgcgaga gcggcgtaga cgtggaacag ccgattgacc tcaccgccgg
     4321 agagcggtgc gcctccccgg cgacggccgc ggaggaggta cgccgatgag tcgatgggtc
     4381 gcgctgaaag gtaacgacgc agcaggtact tgcttcgtgg gtggagcgga tacgcggccg
     4441 atgcgccgtg cttccggcgc gcaatccaga tcttcccatc ttgcacgtcg tcgagcttga
     4501 gcagccctgc ttcgctccgt cgcagcccgt gccggtagat gagatcgaag agcaggaggt
     4561 cgcgtgtctt tcgctgcgaa atcgagtgga agaagcgctc aacctcctct ctcgtgaggt
     4621 acttgaccgc ttcttgctcg cggcgtcgtt catccgccat tccgtcatgt cgctccgcac
     4681 gggcagcgac tctgacactt ttccgcattt gcgttcggtg gggaggcagg ctgaggagga
     4741 agacgcctct cagcctgcct cctgatcgcc ttcctgtaaa cgtcgcaccg ctccaggccc
     4801 acgttccctt actcacgagg aggtaagcgc catgtcgtct caacgagaga attctgcttg
     4861 ctccgctaag acacctaagc gagccttcaa ccatccggat gtcacgaaga acgctggcgt
     4921 agaagtgaag cagctgcgca tccgagagct gtttcttcag cgtgctgagt catacagttt
     4981 gagcgaggct gcgcgaatcg ttggcgtttc gacccgcgcg ctgcggcgtg aagctgaaga
     5041 cgatcagcgc gaagcgtatc tttcgaacgg cacgtggtgt ttcacctggc gacaagttgc
     5101 gttcatcgcc atgcggcggt ggaccctcgc cgagatccat gaggccctcg gggcagaggc
     5161 agagactgtc ctgcctccgc tcttggcgat ggacgtgctc actgttcgtc tcccggcatt
     5221 tctcatccgc gcgatgatga cagccgctga gcaagatgcg atacccctgg atgagtggct
     5281 ccgccttgaa ctcatcgact acgccggaac ggtcgttgac gagatggagg cgattcatcc
     5341 tggcttccga cgggcgtatc tattccctgg tcagaaatag gtcgagaccg gaatggctag
     5401 ccctctgccg cgcataagat gcgtagtggt caccagtcct caaagagcta agaggttatc
     5461 gaccgcacgg gtgtaagcct tccacgcctc ctcgggatcg tcgtggagag caattccgag
     5521 gtattcaaat ttgctccgac caaactccgt gatgcttggc tgtcggccca gcgcctccct
     5581 cagctgaaca tatcgaatgc tcgcttccgc gagatccttc cgcttccagt gtgcattcca
     5641 ctccgcctgt cggcgttctc gttcggggcc atccgtctca tacgcaaagg cggaccagct
     5701 ggtcttcgtt ccgccaatag cctcgaagac gcgaagggca aaggcttgcg tgtcagaggg
     5761 gaggaggcgg gctcgctgtg ttgcaacagg cgatttctcc ccgaaggcgg catagagcag
     5821 gctcacgtca ccgccgaacc agtgattcgc tggtgcctcc gcaaacttgg cgaactgctt
     5881 tgccgttgga gctttgcgct tcacttcgat gtgtcggtta tattcgcgcg cagccaggcg
     5941 tatctcgctc tcccaacgcg cccgaaggta cgcgtcgaga aatttcgtgg cccacgcccg
     6001 acggtaattc gtcacgatgt cgcgaagccg cacgaaacct tcccgtcgtg atccaacgtt
     6061 catcgtcatc gaaatcgtga tcccgcccgc gttgatcttc gagctgtgtt tgggatcagt
     6121 aatcggttcc gggctgccga gcgtgttctc tgcggcgacc agctcagcga acatctttgc
     6181 atcgataggg caaccaagca cattgagcgc gccgatgtcg cgctcgtgat acttctcgag
     6241 tcccgccatg tcggtgcgcg acattggacc ttccgagaca aaccaagccg tcaaacctac
     6301 accgtcccag aaccgcaggg ccggcccgaa cgcggcaagc atgccgccgt acggcccgat
     6361 cgccgtgacg atccgatccg gcgcggaggg catgaacacc ccgctccaca ggcgcgcgat
     6421 gtcggcgagg agtgatgcag gaagtaccgc ccgatattgg ccggggtggt accacggcgg
     6481 accggtttgg gagtcatcaa gccccctgag ccttacggcg ctgtcgtcgt cggcaaatcc
     6541 ccacaatggt tgcataccgg gcgcttgacg cgcgtgtgca tcttcgaagt gttcccaata
     6601 ctcgcgcaac acgcggatgc gctggtggac aggagattcg gtagtgctgc tcgtccagaa
     6661 tgcagcgagc atgcgttcca cttcttcggc tggaacaccg tggcgcgctg caacttcttt
     6721 ggcacggctg gcggctccga catagagccg cggttcgggg agggccgtcg atatctgttc
     6781 gtcggaactc ggcgtctcac cgatgtaggc gaggaaggag agccagtcgc caccaaagaa
     6841 ccctgcgagc gtccgctgca acggcttcgt cagcaccttt cgcatcgtcg gcacgccacg
     6901 agaagtcttc gcgtaatact ttgcgagaag ctcctgcgtc acccacacgc ggtagcgcgc
     6961 gccgatgtcg aagggagcgc tcgcccggag tgcagtaagg agagcgcgtt ttgctacgag
     7021 ttgctcggct cgtcgcagct tgctccagcc gcttccgaac cgcatcgccg cgagcccctc
     7081 tgcgaccggc agtccaagcg catcgtaagt acggtcgatc tcctcccatg catgccgctg
     7141 ctcatcacga tcctgagagg gcggggttgc cccgcgaagc tggaatggcc gatacaaaag
     7201 gtcgaagagc ggattgagga ccgaccagaa gtagagggct tccggcgagg cttggaatcg
     7261 caccttgccg ctctcatcgc gcgcgatccc tgtcgcgaaa cgctcgtatc ggccgaaatg
     7321 gtggggcatg ttggcccagc caacattcag atgggcctca tcagggagga accatgctcc
     7381 gcgcccctgc tgagccgcga gatcgaaggc ttttgcgatg acggcgcgat cggcctcggc
     7441 gagggtcagc aactccaccg tgtcatacgg cttcaccgct tcgcgcgtga cagcgacccg
     7501 tgtcccgtcg agcgaggacc ggatgatccc gctcaagatg ccatcgagct gcgcgtgaac
     7561 agcggcaggt gcgtagccgc gttcaagcgc aagaacatct tccacgctgc actcgcggat
     7621 gcgcgctccg aaggatggct cactcacgga ggttcctcat gatgtcctcg aattgctcaa
     7681 acgtaactgg cgaggacgtg ccttgcgcgt acgaggcata acgctgtgca agcgcttccc
     7741 gctctcgctc gtcgcgccgc tgttccacgc gcacgtcgtc atggacgatg cgctgttcga
     7801 tcgcgcccgg ctggaacaag acaaatgcct ccggccgctc cgccgtgttc gtgccgccag
     7861 cgatcggcaa tggctcgcgc tccgtcgtgg agctgttcca gttcggcggg agcacgtgct
     7921 ctgtgatcgg gtccatgtcg ttctcgcggt agatggtctc gatatgcacg tcgaggccga
     7981 gctcggcgtg gtagatgatg tcgaggaatt gctgcggggg tccgacgcgg cctttgagcg
     8041 cagggtgact gagaacgcga atgccacgcc caacgaactg gtagaactcg ctaaaactcc
     8101 cgtacggtcg catcggaacg acgaccgtaa tcggcgggaa gtcatagccc tgtccgagca
     8161 tcttcaattg cacgatgcct tgaagatcgc ccgactcttg ctcaaagcgc gcgcgaatcg
     8221 ccttgatccg gctcgggctc atggagtggt gcacgtgggc gcaggggatt ccaagctcct
     8281 cggcaatccg tgcgatctgc tccgcatgcc gttcaccgat cgcagaaaag aggacccgtg
     8341 gtttgaccgg ggtgaggagc gccgcttgct gatcgagcgc tgtgcgcacg gcgcgcatca
     8401 cctggcggat cggttcatgc gacttcgccg tgatggtcgc caacttcttc tcatctttga
     8461 gaaggttgag aatcgcctcg cgaccgcgaa tctcctcccg actgccgtcc ggccagacca
     8521 tttcgtagat cgactgcgct tcatcaggcg cgaagcgctg cacgcgcagg ttcttcgcat
     8581 tgccatcggc gatggagtcg atgagacggt agcgatacac cacgtcggcg tcgatcggct
     8641 tgccatcgag ccgctgaaag cacgcagaca tgaggaaggt ccgtgcgccg gcgaagtggc
     8701 ggaagagtcg ctgataggac tcggacgcgg cgatgtgcgc ctcatcaacc acgatcatat
     8761 cgatgtcgtc gggtgcgagt ttcgccaaca agtcatcggg atcatcgccc gtgccgaggg
     8821 aatggaagtt tgtcaggatc acgtccgccg aggtgagcat cgcgtgcgtg atcgagcgaa
     8881 tcgctccctc ttctcggtcg agtgtcagca cctttggtgg cgggcacccc ggaatcagcg
     8941 ggccgttcgg gaacccatac aacgcattcc ccacggcttc gtggtcgagc gccttatcaa
     9001 acgtgccgcg gatgacacta cctggcgtga cgatgagcgc acgtcgctgg gtgaacgcca
     9061 gaatggatgc gacgcccagg gcagtctttc ctgcgccgac cgacatgaca caggcggcgt
     9121 tggtgccgcc gctgcggaag tagtttccga gcgcgctgaa ggcttccgtc tggcattgcc
     9181 tgagcgttcg ctggctgatc ttgtcgcgga cggcatcgaa gtaggtcata ggcgggcggt
     9241 gtagctgcgt tgattctagg gcgcggagcg gattggggtc catgtcagga cgttcgatga
     9301 tgggattctg cacgatgaac tgtcggagga gcggatcgag ctgcaggact gattggagct
     9361 gcgcgacaca ccgggccgta tcgaggcgca ccgcgtcgta ggagaaccga acgacgaggc
     9421 gcgacgtggc tgcgaggtcg ttctgtcgca tgcggtcata cgtgaacgcc gatcggttgc
     9481 tgtgaaactc gaatccatca agttcaatcg cgatgcgctg cgtctctcca acaatctcgt
     9541 agtcgattcg gtatgtgtgg ctgtgcacag cgacgtcggc ttgggcgaca acatgcggtc
     9601 ggatatccgc ggtgaccgcc ggagccaaca cccaccggac gaatgcagct tcgagatagg
     9661 acgcggctga aaggtcgaga gccatcgatt caggattcat cctcggcgtc gagcacagcg
     9721 atgcgttcgg tcagccgttc ttcatcggaa gagtcctggc cggctttcac cgctcgcttt
     9781 gcaatatcga gcgcctcgtg aaggaatccc tcgcgctcat acagtgcaac gaggtactca
     9841 aacgctggta catgtggaac gaactcctcg ccctgagtat caaacgtgat gctcttcgga
     9901 tgcgcttcga cgagtgagat atcgagcagg aggatctttt cgagttcagg gaggatctcc
     9961 ggctcacgtt tgcggtgtgc ccagagtgct tggtacacgc caagcatcgc gaagtgataa
    10021 tcagagatgg tgcctggaag ctcgatagcc tcatacagcc gccgaagaag ctgtggcgct
    10081 gaggtcttgc gcggatcgtc cccgccgctc caccccgcag cgggggatcg agacgtggac
    10141 ccactttcgg tcttccaaga aagagatcgc tgggtccgtc cgtcaaagac gaaagactcc
    10201 acagaaggct tggcgttgtg ctggggaagc acacgccgat caacgtcggg atgcccttca
    10261 tatgtgatac cgggaacgtt cccgagccgc tcataccagt ggatgtcgtc catcgaattc
    10321 aaggatagcg gtcaacccgc catcctccaa cttcttgcca gaacgcaaaa ccgcctgggc
    10381 atatggttga agcctagtta ctcgggcctg ctcgttcggc gtcctcagcg acgatgcgaa
    10441 ccgcgccttc gagctgacca tctcgcagcc gatagcatcc actgatgatg taaggggcgg
    10501 tacggtgctc tcccagcgga gcgagcacgc gcttctcacc gagaacggtc cagatcatcg
    10561 ccagtccttg cttctcaagc atggcagaga gccgcgcgcg atcaacaaca actgcgcccg
    10621 gtcctgcctc aaagaccgac ggatcgaagg ctacttcggc atcgtgctgg tccacgaagt
    10681 aaccctccct gccacgccac gtgagaccca tccactccac caactcggca gccggaagat
    10741 ggatgttcac ggtttcatcc agcgagcaat cgaaggtgct gtgctcgcac atataagcat
    10801 cagtggagac gagaaccggc gcgggaagtc gactgtcttc ctcatcgccg ccaacccacg
    10861 cccgccgtcc cgtgtggcgc gtggagtggt agtggaatgc cggtgcccaa aacagctcgc
    10921 cgaaatagac gcgggtcatt tcgtgcgact ccggcatcca tcggccgtag aaattctgtt
    10981 gtgagcccca ctgatagaag gcatcggcgt gctcgcgccg cactacgtag gacttgacca
    11041 tgtaccaaat ctcgcgccgc tcaatgtcat agcgctcgac gcccggcggc gcaggttctg
    11101 tccactcgcg ataggtctca agtaccagaa actctctacc atctggccgc tgagcagcaa
    11161 tcagaggttc gacaggcggc aagtcggaac tctccgcgac ccacgccgca tcgctaccga
    11221 tcgatcgcca gtcgccatac ggcatcagtg gagaccacca cgcggccgca gacgtggaac
    11281 gctcgctagc ggtcgcggcg atgaggagcg aggggtcgag atcacgatcg aagtcctgcc
    11341 acgggccatc gtacttgcgt acctcgcggg ccatgtggtc ttcgtactgg aagtgatcgg
    11401 ctacacgcgc caggaactcg tgccacgcga gccattgata cttcttcccg atccgctccg
    11461 gcttatttgc ttcccggccg acgctcccgt ggcggagatt gcggtcgaag aagccgaatc
    11521 gctcaatcgt ccacccgagc tcaaagacgc ggttcaaaac aaaacgctgc gcaatcccga
    11581 gatcgaagag atgttcttcc tccttccccg aatgttcgat gtacggaagt gcctccgtct
    11641 cgaaccgctc tagttcctcc gggccgagca tcttgcgaaa tgcattctca gtatccgcga
    11701 tctcgcgttc cagttcttgc tcgatgtgct ctcgctcctc gtcgcttacg cgcctgaatg
    11761 cgcgcggtgg tcgaggctcg tctgcgccct ccggattgct tgtctctgtc tcttcctcgg
    11821 gctgctgttg atacgaggca gtaagtaact tgaaaaacgc gccgtgtgaa gccgtctcaa
    11881 agcgctcaag cgcctctcgt tgctcgtcgg tgaggctttc agcgaatgca tcatgccgtt
    11941 cgcgtttcgt gggcggtacc gattcgtcga gtcggattgc gctccaattg aaggagtggc
    12001 tgttcgtgcc gatgacgtac ctatcgaaat cgccgccgcc ggcaacggag aaatagatcg
    12061 tactctgcgc gtatccttca tcgcccgatc cctctggtcg ctcgtcgtac ttcttcttga
    12121 gttcctccgc ggaggggaga ttctccggcc agtcgctctt atacggtgga cgtacgcggc
    12181 caagatcgag ccctccatct actccgagcg cagcggcgcg ctcgacgaca ccacgtgcgt
    12241 agtcgcgaag gaggatgtgt ggcgtcggtg tgccgtcttt gaatacgtct tcaaatacgt
    12301 gttgggcaat gccgccgatc gcattgccat cgctgctcct gaggacacat ccgtaggcaa
    12361 tgccatacag ccgctcgcga acgtagggat catccacgtc tcggaaggcg gtcaggagac
    12421 tttcgagcac agggagacgc ggcgtgagaa ggcgaacgag cgccttcgtt gcacggtctc
    12481 gcagcggtcg gtgcgaggtg gtgagaaacc acatcagcgc ggttgcggta agacgtaccg
    12541 actcatcatc gatatggctc ttatcttccg atgaccatgc ccactccacg aggcggtcaa
    12601 cggcggtgtg ctccccatac tgctcagtca agtagatcga ccaccatgca tcccgttctg
    12661 gcatggggtc ccgcatcaga cgtgcgtgca gataatcggc attgaaggga tgatcgggat
    12721 tcgcagctac cgtgagtacc acgtcgagca gctcgtcaaa ctctgcctgc gtaagacctg
    12781 cgcggagata cttcttcgca ggttcgccga tggcctgcgg ccgccgccat agaaggctgc
    12841 ggataaatgc gcgtctgatc ggcgaggagt cagccgcatc gggcatcacg tccgcaagct
    12901 cgacgccgaa ccgctctgga acctggatcg acagcgcctc gatgagccca aaggtccatt
    12961 cgcgtgtgta ctcgcgcacg cgagcgccca acggctgctc cggttcgagc gcagtggcga
    13021 tatcgtcttc cgctacgtgc atgtcgatga gatagctcgc gatctggtga tcctgtagcc
    13081 gctcgtacgc gaagcggatt ccttcgcgaa ggccctccgg tcgccacacc cgctcctccg
    13141 tcagcaggcc ctcagataag agccgcgcga agagcgaatt gttatacgtg cgattcggca
    13201 agtgcgcatc caccgtcgcc tgtgcttcgt tacgcgtgag aaagtgactc gccgacgaag
    13261 cgagttgctg cgcgacgctg cgcaacgcct cgtggacaag gtgtttcttc gggtcgaaat
    13321 ccaactggtc cggcccgctc aacttttggt tcactgcatc gaggaggaag tcgaacatct
    13381 gcgtgacgcc gtgcagcccc ttcggcatct ccgtcaagcc ggcgtcctta atccctttgc
    13441 agaacagctt gaggaagagt ggattctgga actctggatt gagcagcgga atggagggac
    13501 gcttgatgcc aaaccactcg aagaacgtcc gggtcgcctg atactcgtgc gccgcgaagc
    13561 cggggtgtgt ttcgcgggtg aggtgctcac cgagactctc cggcacaacg tcgaccgtat
    13621 aggacgtgc
//
LOCUS       DATLVW010000659         3556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_398063, whole genome shotgun sequence.
ACCESSION   DATLVW010000659 DATLVW010000000
VERSION     DATLVW010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398063"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..2318)
                     /locus_tag="VNN25_19140"
     CDS             complement(150..2318)
                     /locus_tag="VNN25_19140"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXH93705.1"
                     /translation="MRRVAALVITLFTASTLFAVAPQFWRVRNAEDFLAGDIEGFAVT
                     SRGELRPGPSLKKIATFTDPFVLSQVGAPNGDHFFGTGNEGKVYRLHGTELKAVYTAP
                     EPEVYAVAFHDGAVYVGSSPNGKVYRVDPNDGKSTVFFDPKQAYIWAMEFLPNGDLAV
                     ATGVDGKLFRVTPKGEGKVLFDSPETHLRCLAVKKDGTLLAGGSAKGRIYEIRPDGGA
                     HALYDSPLSEISSIYVDANGIGWASAASNILPSTAPVKATAPKPATQTTAPSASTTAS
                     SGEQKKEEPTGSVEVSIGIDDSAAGSAGSGEIYKINPDGFVELVRKFEHEMAYGITGG
                     PNGSILLATGPQGRIYQLNDGEVSLVGSVPEKQVVSISTVAGQTLITTTNSGAVYRME
                     SGPSAKAEFRSAAKDVERFSRFGHYRIEGDNLGDGHLAIAFRSGNTRTPDATWSPWSA
                     ASSTSDGPVDVPAGRYVQWKLTMPKPVANVSVDEVTVAFMNRNVAPVIDTVVVQDPAV
                     VYITGSYPQSPQVVEATNPDEYGIFTSLDTPRDRNEQGKKVYRRGYRTVTWRAHDDNG
                     DALRYTLSFRKVGSEKWLRLRENFEESSLNFDTSQLPDGNYELRLSVTDAQDNPEHGL
                     TDVKEGIQIVVDNTPPAITATNEGDDVVIHVSDKRSPIGKVEYSADAEKWIRLTPVDG
                     IGDSPNETYRIKRSAVAGKFVIVRAVDAFFNVGTESIALP"
     gene            complement(2601..>3556)
                     /locus_tag="VNN25_19145"
     CDS             complement(2601..>3556)
                     /locus_tag="VNN25_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93706.1"
                     /translation="LASSFKFANTGAVVGAFRQDRAVGIMGMMGEKADMIPVDLEVEG
                     SGAAQKYHVDVLRHSQLTPLILAMAADTVVASAQRAAGERTVMLESEIKIKGYPEPIV
                     LREGWAGAQARQAIPSYLAVVAGYVMSNEFHTADIESVKIHFRHTDDLKIAKILEASL
                     AMPSNGHIHAGDTVAVRAVLKPYRGEAFVDTFDVKIPDDQAAGTAYLLVGSGSVLNQV
                     DFMLVPPDPRSLDQVIAVLRRLRPSTELTLGLYEPTDGAVTAGVYLPNLPPTMRAIVS
                     SDTSNGPQAPVRYHASQHIAHPLGYIVDGVVKIDLDVKPTI"
BASE COUNT          641 a         1069 c         1127 g          719 t
ORIGIN      
        1 ccgcaaccaa atgaaaccca ccaaatctgc aaccaatcag ttttggtgaa aatctttgcg
       61 tcgcgcgcag aaaccgctgg ttagtagtac agagggcaca gagttggatt ctgctctgtg
      121 tcctctgtgt ctctgtggtg aaaacgcttc tacggtaacg cgatcgactc cgtccccaca
      181 ttgaagaacg catcgacagc gcggacgatc acgaacttgc cggcgacggc gcttcgtttg
      241 atgcggtagg tctcgttcgg ggaatcgccg atgccgtcga ccggcgtgag gcggatccac
      301 ttctccgcgt cggctgagta ctccaccttc ccgatcggcg agcgcttgtc cgacacgtgg
      361 atgacgacgt cgtcgccctc gttcgtggcc gtgatcgccg gcggcgtgtt gtcgacgacg
      421 atctggatgc cttctttgac gtcggtgagt ccgtgctcgg gattgtcctg cgcgtcggtc
      481 acggacaaac gcagttcgta gttgccgtcc ggcagttgcg aggtatcgaa gttcagcgac
      541 gactcctcga agttctcccg caggcggagc cacttctcgc tccccacctt gcggaacgat
      601 agtgtgtagc gaagcgcgtc gccgttgtcg tcgtgcgcgc gccaggtcac ggtgcggtaa
      661 ccgcggcgat agaccttctt gccctgctcg ttcctgtcgc gcggcgtgtc gaggctggtg
      721 aagatgccgt actcgtcggg attcgttgcc tcgacgactt gcggcgactg cgggtagctg
      781 ccggtgatgt agacgactgc cggatcctgc acgaccaccg tgtcgatgac cggagcgaca
      841 ttgcgattca tgaaagcgac cgtcacttcg tcgaccgata cgttggccac aggcttcggc
      901 attgtcaact tccactgcac gtagcgtccg gcaggaacgt cgaccggacc atcgctcgta
      961 gacgacgctg cgctccacgg actccaggtc gcgtccggag tgcgcgtatt gccggagcgg
     1021 aaggcgatag cgagatgacc atcgccgagg ttgtcgcctt cgattcgata gtgtccgaag
     1081 cgcgagaaac gctccacatc tttcgccgcc gatcggaact ccgctttggc cgatggccca
     1141 ctctccatcc ggtacaccgc gccgctgttc gtcgtcgtga tgagcgtctg gccggcgacg
     1201 gtcgagatcg agacgacctg cttctcggga acgctgccga cgagcgagac ctcaccgtca
     1261 ttcaactggt agatgcggcc ttgcgggccg gtggcgagga ggatcgatcc gttcgggccg
     1321 cccgtgattc cgtacgccat ctcgtgctcg aacttgcgga cgagctcgac gaagccgtca
     1381 ggattgatct tgtagatctc gccgctgcct gcggaaccgg ccgctgaatc gtcgataccg
     1441 atgctgacct cgacgctgcc ggtcggttcc tctttcttct gttcgccgga tgatgcggtg
     1501 gtcgatgccg agggcgcggt tgtttgcgtt gctggcttgg gcgcagtcgc tttcaccgga
     1561 gcggtcgacg gcaggatgtt gcttgcggcc gacgcccacc cgatgccgtt cgcgtcgacg
     1621 tagatcgatg agatctcgga caacggcgag tcgtacaacg cgtgcgctcc gccgtcggga
     1681 cggatctcgt agatgcgtcc tttcgccgat ccgccggcga gcagcgttcc gtctttcttc
     1741 accgccaggc agcgcagatg cgtctccggc gagtcgaaga gaaccttccc ttcgcccttc
     1801 ggcgtgacgc gaaacagctt tccatcgacg ccggtggcga ccgcgagatc gccgttcggg
     1861 aggaactcca tcgcccagat gtaggcctgc ttcggatcga agaagaccgt cgacttgccg
     1921 tcgttcggat cgacgcggta gacctttccg ttcggcgagc ttcctacgta caccgccccg
     1981 tcatgaaacg ccaccgcata aacctcgggc tccggcgccg tgtagaccgc cttcagctcc
     2041 gtgccgtgca ggcggtagac cttcccttcg ttgccggtgc caaagaagtg atcgccattc
     2101 ggcgcgccga cctgcgatag gacgaacgga tcggtgaacg tggcgatttt cttcagcgaa
     2161 gggccgggac gaagctcgcc gcgcgaggtg acggcaaagc cctcgatgtc gccggcaaga
     2221 aagtcttcag cattgcgaac acgccagaac tgcggcgcaa cggcgaagag ggtcgaagca
     2281 gtgaacagcg tgattacgag cgcagcgact cggcgcatga aagttccttt agctacagaa
     2341 gtgtgagagt caggctggag acaagggatc agagacaaga gatcagggca gaagatcaga
     2401 gacgagagat tggagacaag agatcagggc aatcgtgcgc attctagttc tctgattccg
     2461 gcctctgatc gttggtcgac ttacattttg cgaaacttag agacaagaga tctcgccaac
     2521 ttctacggag atccgcacat tctggttact gatctctagc ccccctgatc tctgatcgct
     2581 gatatccctg atctctgcct ctaaatcgtc ggcttcacat ccagatcgat cttcacgacg
     2641 ccgtcgacga tgtagccgag cggatgtgcg atgtgctgtg aggcatggta gcggacgggg
     2701 gcttgcggtc cgttcgacgt atcgctgctg acgatggccc gcatggtcgg aggtagattt
     2761 gggagataga cgccggctgt gacggcgccg tcggtcggtt cgtagaggcc gagggtgagc
     2821 tcggtggatg ggcggagccg gcgcagaacg gcgatcacct gatcgagact gcgcggatcg
     2881 ggtggcacga gcataaagtc gacctgattc agcactgacc cgctgccgac gaggaggtac
     2941 gcggtgcccg ctgcctggtc gtcgggaatc ttgacatcga acgtatcgac gaacgcctcg
     3001 ccgcgatacg gcttgagcac ggcgcgcact gccacggtgt cgccggcgtg gatgtgtccg
     3061 ttcgacggca tcgccaacga ggcctcgagg atcttggcga tcttgaggtc atcggtgtgg
     3121 cggaagtgaa tcttcaccga ctcgatgtcg gcggtatgaa actcgttcga catgacatag
     3181 ccggcgacga ccgcgagata cgacgggatg gcctgccgtg cctgcgcgcc ggcccaacct
     3241 tctcgaagga cgatcggctc ggggtagccc ttgatcttga tctccgactc cagcatcact
     3301 gttcgttcgc cggcggcgcg ctgggcgctg gcgacgacgg tgtccgcggc catggccagg
     3361 atcagcggcg tgagctgcga gtggcgcagc acatcaacgt gatacttctg cgcggcgccg
     3421 ctcccttcga cttcgaggtc gaccgggatc atgtcggcct tctcccccat catgcccatg
     3481 atgccgaccg cgcggtcctg acggaacgcg ccgacgacgg caccggtgtt ggcgaacttg
     3541 aacgagctgg ccagac
//
LOCUS       DATLVW010000660         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_398069, whole genome shotgun sequence.
ACCESSION   DATLVW010000660 DATLVW010000000
VERSION     DATLVW010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398069"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VNN25_19150"
     CDS             <1..181
                     /locus_tag="VNN25_19150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXH93707.1"
                     /translation="FRYVLLVAKRAEQLVQGSMPKSKSRHAKPTRIAMEEIEKNQVKW
                     QLTPPEEETTALETE"
     gene            373..1557
                     /gene="coaBC"
                     /locus_tag="VNN25_19155"
     CDS             373..1557
                     /gene="coaBC"
                     /locus_tag="VNN25_19155"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXH93708.1"
                     /translation="MHIVLGVSGGIAAYKAPDVVRRLRDVGADVRVILTPNAARFVSP
                     LSLAAVSGHGVIIEQWGDSGHGGVDHIDLARWADLLLIAPATANILAKLAVGIADDAL
                     STYAIAHRGAIVVAPAMNTSMLQHQTVQMNMAYLRERGVIVLDPDSGLLACGDEGSGR
                     MPDPPVLAEFVKQQFAALDLEGKAILVTAGPTREPIDPVRYISNRSSGKMGYAIAEAA
                     RRRGAKVTLVSGPTAIPAPAGVAITRVTTAAEMHAAVMEALPAQQIVIKAAAVADFTP
                     VTVSTQKIKKQAGVEETTLTLRKTPDILAEIGATSPRPFVVAFAAETDSVEASAREKL
                     VRKNADLIVANDVADPSIGFDSDQNAVTVIARDGSSTNIERAPKIVVANRILDLIVQR
                     LA"
     gene            1579..>2134
                     /locus_tag="VNN25_19160"
     CDS             1579..>2134
                     /locus_tag="VNN25_19160"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXH93709.1"
                     /translation="MNDELRLELTLLRDLGFTHLDLRNAPPPAVQDDARSLEAKTLLG
                     ELEAIASVCVKCELAKTRTQVVYGVGNPNADLMFIGEAPGRDEDEQGKPFVGRAGQLL
                     TDIIKAMKLTRDDVYIANVIKCRPPENRNPEPDELDACRPYIRQQVEIIQPRVIVTLG
                     KFALQSLLEKAYGITAVRGKWLEYQ"
BASE COUNT          417 a          694 c          671 g          352 t
ORIGIN      
        1 attccgttac gttctgctcg tggccaagcg cgccgagcag ctcgtacaag gctccatgcc
       61 gaagagcaag agcagacacg ccaagccgac gcgcatcgcc atggaagaga tcgagaagaa
      121 ccaggtgaag tggcagctca ccccgccgga agaagaaacg accgcgctgg aaactgagta
      181 ggcacgggga gtggggggtg gggtgtgggg gagaagcacg cagcttacgc gcgtctctcg
      241 catgcgttac tcaccactcc gcgatcgttg tagagaaggg aagtcgggtg tcgtcgggtg
      301 tcgcagaaaa gcaggcgtga tctgcttctc tctcccaccc cccaccccct actccccacc
      361 ccccacaaac acatgcacat cgtcctcggc gtctccggcg ggatcgcggc gtacaaagcg
      421 ccggatgtcg tgcggcgcct gcgcgacgtc ggcgcggacg ttcgcgtcat cctcacacca
      481 aatgcggcgc gcttcgtgtc gccgctctcg cttgcggccg tctccgggca cggcgtcatc
      541 atcgagcagt ggggcgactc cgggcacggc ggcgtcgatc acatcgacct ggcgcgctgg
      601 gccgatctgc tgttgattgc gccagccacg gcaaacatcc tcgccaagct cgccgtcgga
      661 atcgccgacg atgctctctc cacgtacgcc atcgcgcatc gcggcgcgat cgtggtcgct
      721 ccggcgatga acacctcgat gctccagcat caaaccgtgc agatgaacat ggcctacctt
      781 cgcgagcgcg gcgtgatcgt tctcgatccc gacagcggtc ttctggcctg cggcgacgaa
      841 ggctcgggac ggatgcccga tccgccagtg ctcgctgagt tcgtgaagca gcagttcgcc
      901 gcgctcgacc tggaagggaa ggccatcctc gttactgccg gtccgacgcg cgagccgatc
      961 gatcccgtcc gttacatctc caaccgctcg tcgggaaaga tgggatatgc catcgccgag
     1021 gcggccagga gaaggggagc gaaagtgacg ctggtctcgg ggccgaccgc aattccggca
     1081 ccggcaggcg ttgccatcac gcgcgtcacc acagcagcgg agatgcacgc cgcggtgatg
     1141 gaagcgcttc ccgcgcagca gatcgtgatc aaagcggccg cggtggccga cttcacgccg
     1201 gtaacggtct cgacgcagaa gatcaagaag caggctggcg tagaagagac cacgctcacg
     1261 ctcaggaaga cgccggatat ccttgccgag atcggcgcca cgtcgccgcg tcctttcgtc
     1321 gtcgcctttg ccgcggagac tgactccgtc gaggcgagcg cccgcgagaa gctcgtccgc
     1381 aagaacgccg acctcatcgt ggccaatgac gttgccgatc cctcgatcgg tttcgattcc
     1441 gatcagaacg cggtcacggt catcgcccgc gacggatcgt cgacgaacat cgagcgtgct
     1501 cccaagatcg tggtggccaa ccgcatcctc gatctgattg tgcagcggtt ggcctgaccg
     1561 agggataatt cgcggctgat gaacgatgaa cttcgtctcg agctgaccct cctccgcgac
     1621 cttggcttca cccatctcga tcttcgcaat gcgccgccgc ccgcggttca ggacgacgcg
     1681 agaagcttgg aggcgaagac gctcctcggc gagctcgaag cgattgccag cgtttgcgtg
     1741 aagtgcgagc tggcgaagac gcgcacgcag gtcgtgtatg gcgtcggcaa tccaaacgcg
     1801 gacctcatgt tcatcggcga agcgccggga cgcgacgagg acgagcaggg aaaaccgttc
     1861 gtcggccgcg ccggccagct tctcaccgac atcatcaaag cgatgaagct cacccgcgac
     1921 gatgtctaca tcgccaacgt catcaaatgc cgtccgccgg agaatcgcaa tccggagccc
     1981 gatgagctgg acgcctgccg gccgtacatt cgccaacagg tcgagatcat ccagccgcgc
     2041 gtgatcgtga cgctggggaa gttcgccttg caaagcctgc tcgagaaggc gtacggcatc
     2101 accgcggtac gagggaagtg gctcgagtat cagg
//
LOCUS       DATLVW010000661        10098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_26282, whole genome shotgun sequence.
ACCESSION   DATLVW010000661 DATLVW010000000
VERSION     DATLVW010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26282"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4101
                     /locus_tag="VNN25_19165"
     CDS             <1..4101
                     /locus_tag="VNN25_19165"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93710.1"
                     /translation="PNGFTSVQQNPTRANATTSDAGTYSVTVTVSGCSSAAGTTDVAV
                     NPAPATPTASNGGPYCEGATISLSTPTVASATYAWTGPNGFTSTDQNPTRTNATSADG
                     GTYSVTVTVNGCPSAAGTTSVVVNPTPATPTASNGGPYCAGATISLSTPAVSGATYAW
                     TGPNGFTSALQNPNRSNATTADAGTYSVTVTVSGCTSAAGTTGVVVNPIPATPSASNG
                     GPYCESGTISLSTPTVAGATYAWTGPNGFTSAQQNPTRANITTADAGTYSVTVTVSGC
                     TSAAGTTNVVVNPTPATPTASNGGPYCEGATISLSTPTVSGATYAWTGPNGFTSALQN
                     PTRSNSTVADAGSYSVTVTVNGCPSAAGSTSVVVNPIPATPTASNGGPYCEGATIALS
                     TPTVSGATYAWTGPNSFTSALQNPTRSGATTADAGSYSVTVTVNGCTSAAGSTTVVVN
                     ATPATPTASNGGPYCEGGTISLSTPTVSGATYSWTGPNSFTSALQNPTRSNATVADAG
                     SYSVTVTVNGCASAAGSTTVVVNATPATPTASNGGPYCEGATIQLSTPTVAGATYAWT
                     GPNSFTSALQNPTRSNATVADAGSYSVTVTVNGCASAAGSTTVVVNATPATPTASNGG
                     PYCAGATIALSTPTVGGATYSWTGPNGFTSALQNPTRSNATAADAGTYSVTITVNGCT
                     SAAGTTSVVVNPAPATPTASNGGPYCEGATISLSTPTVGGATYSWTGPNGFTSALQNP
                     TRSGATTADAGSYFVSVTVSGCTSAAGSTSVVVNATPATPTATNGGPYCEGATISLFA
                     PTVAGATYSWTGPNSFTSSQQNPTRSNATVADAGSYSVTVTVSGCTSAAGSTSVVVNA
                     TPATPTITPGGPTTFCAGGSVTLTSSAASGNQWYLNAGLITGATGNTYSAVASGNYTV
                     VVTTSGCSSAPSAAISVTVNPAPATPTITPGGPTTFCNGGSVLLTSSSATGYQWFLGG
                     NPITGATNQTYSATASGNYTVVVSNGCNSAPSAPTTVTVNPIPATPTISAGGSTTFCE
                     GGSVTLTSSSATGNQWYRNGVLLGSETSQTTVATLAGAYSVTTTASGCTSAQSAATII
                     TVNPKPNVTISVPSPMYSGASGTASVIHECTGATFAWSIIGGTITSGNGTRIITFTAG
                     PAGTLTLFVTVTNSFGCSDSKTADVTVQQAPFGAPPYFSAAATSTTSVSMLWATVATA
                     DHYEIYRSTDNTNWTLRGTNTLATFSESGLTPSTTYLYKVRAIKADTTASGYSAIDPA
                     TTIVFTDPSLIACPTLIRPIHITELRTAINAARASVGLTAFAFTDPSLGAGAQIKAVH
                     VTELRTALAPVLSAIGVTPTYTDPTITAGTTHVKGTHIQELRDLIR"
     gene            complement(4273..4587)
                     /locus_tag="VNN25_19170"
     CDS             complement(4273..4587)
                     /locus_tag="VNN25_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93711.1"
                     /translation="MSEQIIPMPEQIIPMSEQIIPMSEQIIPMPEQIIPMSKRIIPMS
                     KRIIPMSERIIPMPEQIIPMSKCSVPISQSPFPTARQARRTPTPSQATCELLENTADA
                     LI"
     gene            complement(4882..5526)
                     /gene="eda"
                     /locus_tag="VNN25_19175"
     CDS             complement(4882..5526)
                     /gene="eda"
                     /locus_tag="VNN25_19175"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXH93712.1"
                     /translation="MSAVDRIREVGIVPVVRATSAAEALEAVEAIRAGGIPILEITLT
                     VPGAVKIIAELTGRFGDETLIGAGTVLDAESAQACIDAGARFVVSPSLDIPTIELCRR
                     LGVAIFPGALTPTEIVTAWKAGADAVKVFPANALGGPTYLKSIKAPLPQIALIPTGGV
                     SLKNAADFIAAGAFALGVGADLVDLAALRRGEASSITEKAREYVAAVASARGSA"
     gene            complement(5513..6601)
                     /locus_tag="VNN25_19180"
     CDS             complement(5513..6601)
                     /locus_tag="VNN25_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXH93713.1"
                     /translation="MKINDAADCRFDLVSLGEVMLRLDPGDGRVSTARSFQVWEGGGE
                     YNVARGLRRCFGLRTAIATALVDNPVGRLVEDLMLQGGCDLSLLRWAADDGVGRSARN
                     GLNFTERGFGVRAAMGCSDRGHTAISQLKPGDIDWESLFASGVRWFHTGGIFAALSET
                     TPLVAREAMEAARRHGVIVSYDLNFRPSLWKGIGGAEKARAVNRELVSFVDVLFGNEE
                     DFSVALGFNIEGVDEHHATLPADAFRQMIGRVCAEFPALQVVATTLRVAHTATRNDWG
                     AIAWCDGEFHEALTRTDLEVLDRVGGGDSFASGLIYGLLTGRGPAWSVECGAAHGALA
                     MTTPGDTSMATLAEVERVMKGGTARVSR"
     gene            complement(6598..7419)
                     /locus_tag="VNN25_19185"
     CDS             complement(6598..7419)
                     /locus_tag="VNN25_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HXH93714.1"
                     /translation="MTVDLAAIEPRTCVVRDTGARRGRHITVTPETTSARHLHFGRIV
                     LEASDAAVTFSTGELETAFICLKGTATIAAGGTTYDLARYDALYVPRDSDVTVSGNCD
                     VAELAAPVDNRYPIQLVRFADVQNDASLHFHTGGPSSERDVNIVIGKNIDAGRILAGV
                     TFSHPGNWTSWPPHEHARMLEEAYLYIDMPEPGWGLQLVYNDPRNPELVAVVREGDVV
                     LMPQGYHPNVSAPGGTINFLWMMAAIREGDDRQFGVVNVQPEYAQGGSGLEASRK"
     gene            complement(7416..8801)
                     /locus_tag="VNN25_19190"
     CDS             complement(7416..8801)
                     /locus_tag="VNN25_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HXH93715.1"
                     /translation="MTELARLLRTLLALSTLLVSVSATAETTDPWAAVPGILARIVPP
                     SFPSRDFVITKFGAHDGADASVAIAKAIDACAHAGGGHVIVPKGDYITGPIRLQSHVD
                     LHIDAGATLRFSQDPSRYPLTLTRWEGVELMNYAPLISALDCEDVAITGEGTLDGQAD
                     ETHWWNWKGEKEPRLQSADRNALFKQNDEAVPVAQRVYGSGHFLRPTFVEPLRCTNIL
                     IEGVTIRNAPFWVLHPTLCTNVTIRRVTVFGHGPNMDGCDPESCRDVLIEDCVFDTGD
                     DCIALKSGRNADGRRLNVPIENVVIRGCRMNGGHGGITVGSEISGGARNIYAEKNRLD
                     SPILERGLRIKTNSARGGIVENIFARDIEIGTLMFAPIEIDLRYMSETGTFVPTVRNI
                     VVERMHSMHSQYGLYIRAFDKTPLRGVVVRDSAFRGVANGHVIEGEVDLTLTDVTVET
                     APAPKPEKRKP"
     gene            complement(8905..>10098)
                     /locus_tag="VNN25_19195"
     CDS             complement(8905..>10098)
                     /locus_tag="VNN25_19195"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HXH93716.1"
                     /translation="GYPSKVRLPRLLRHLLPAAIMAAGAFLTGARNLQAASAPAELRV
                     IVRNALPIARSSETVEIAASLFVPLGGDLTRVHVFDHDTGREVLAQALDLDGDGKQES
                     LIFQTDMKPMGARSFDLRTGERVTYKKEDFHVYGRFVRERYDDFAWENDRVAHRMYGT
                     ALETWQKEPLTSSTVDVWLKRTRRLIVNDWYMVDDYHRDTGEGADFYSAGRSRGCGGS
                     GLWRDGKLIVSKNFRNSRVLANGPIRLVFELDYPIWDVAGVRSETKRITLDAGQNFNR
                     FESRYVVDGVNDPTYATGIKKFAATDVRMERSAGWIRAWGPVVGGTGKFGCAIVIDPA
                     AIVDVTEADGNHLIVAKTPAVYYAGTGWDKSGDFADVSDFDKYVEQFAQRVKSPLRVE
                     VVDGR"
BASE COUNT         1955 a         3412 c         2985 g         1746 t
ORIGIN      
        1 ccgaacggct tcacctcagt acagcagaac ccgacccgcg ccaacgccac gacttcggat
       61 gccggaacct actcggtgac ggttaccgtc agcggctgct cctcagctgc cggcacgacc
      121 gacgtagcgg tcaacccggc accggcgaca ccaaccgcgt cgaacggcgg tccctattgc
      181 gaaggcgcga cgatctcact gtcgactccg actgtggcca gtgcgaccta cgcctggacc
      241 ggcccgaacg gcttcacatc gaccgatcag aatccgacgc gcacgaacgc cacctccgcc
      301 gatggcggca cgtactctgt gacggtgacc gtcaacggct gcccctcggc agcgggcacg
      361 acttcggtcg tcgtcaatcc gactccagcc acacccaccg catccaacgg tggcccgtac
      421 tgcgcaggcg ccacgatctc actctcgacg ccggccgtga gcggcgcgac ctacgcgtgg
      481 accggcccga acggcttcac ctcggcattg cagaatccga accggtcgaa cgccaccacg
      541 gcagatgcag ggacctactc cgtcaccgtc acggtgagcg gatgcacatc cgccgcaggg
      601 acgacaggcg tcgtcgtcaa tccgattccg gcgacgccaa gcgcatcgaa cggaggcccc
      661 tactgtgaga gcggaacgat ctctctctcc actcccaccg tcgccggtgc aacctacgcg
      721 tggaccggtc cgaacggctt cacctcggcg cagcagaacc cgacgcgcgc caacatcacg
      781 acggcagacg cgggaacata ctcggtgacg gttaccgtca gcggctgtac ctcggccgcc
      841 ggcacgacga acgtcgtggt caatcccact cctgccacgc caaccgcgtc gaacggcggt
      901 ccctattgcg aaggcgcgac gatctctctc tccacgccga ctgtgagcgg tgcgacctac
      961 gcctggaccg gcccgaacgg atttacatcg gcccttcaaa acccaacgcg ttccaactcc
     1021 acggttgccg atgcaggaag ttactccgtc acggtgaccg tcaacggctg cccctcggca
     1081 gcgggatcga caagcgtcgt cgtcaatcca atcccggcca cgccaaccgc ttcgaatggt
     1141 ggaccgtact gcgaaggcgc aacgatcgcg ctctccacac caaccgtgag cggcgcgacc
     1201 tacgcctgga ctggtccgaa cagtttcacc tcggcgctgc agaaccccac tcggtcgggt
     1261 gccacaacgg ctgatgcagg cagctactcc gtcaccgtga ccgtgaacgg ctgcacatcc
     1321 gcggcaggat cgaccaccgt cgtcgtcaac gcaacgccgg caacgccgac agcctctaac
     1381 ggcggaccgt attgcgaagg cggcaccatc tcactctcca cgccgacagt aagcggtgca
     1441 acgtattctt ggaccggacc gaacagcttc acgtcggcgc ttcagaatcc cactcgctcc
     1501 aacgccaccg tcgctgatgc gggcagctac tccgtcaccg tgaccgtgaa tggctgcgcg
     1561 tccgcagccg gatcgaccac cgtcgttgtc aatgcaacgc cggcaacgcc aacagcttcc
     1621 aacggcggac cgtactgcga aggcgcgacc atccagctct cgaccccgac tgtcgccggt
     1681 gcgacatacg catggacagg cccgaacagc ttcacgtcgg cgcttcagaa tcccacgcgc
     1741 tccaacgcca ccgtcgctga tgcgggcagc tactccgtca ccgtgactgt gaacggttgc
     1801 gcgtccgcag ccggatcaac caccgtcgtt gtcaatgcaa cgccggcaac tccaacggct
     1861 tccaacggcg gcccctactg cgcaggcgcc acgatcgcgc tctccactcc gacggtcggt
     1921 ggtgctacct attcatggac cggtccgaac ggattcacct cggctctaca gaacccgacc
     1981 cgctcgaacg ccacggccgc cgacgcggga acctactccg tgacgatcac cgtcaacggc
     2041 tgcacctccg ctgccggaac gacgagcgtc gtcgtcaatc cagcacccgc cacgccgacg
     2101 gcttccaacg gcggaccgta ctgcgaaggg gcaaccatct cgctctccac gccaaccgtc
     2161 ggcggtgcaa cgtattcatg gaccggtccg aacggcttca cgtccgcgct acagaacccg
     2221 acgcgctccg gagcgacaac tgctgatgcg ggcagctact ttgtatccgt gaccgtgagc
     2281 ggctgcacct ccgcggccgg atcgaccagc gtcgtcgtaa acgcgacgcc agccacacca
     2341 accgccacca acggcggacc gtactgcgaa ggcgcgacca tctctctctt cgcgccgacc
     2401 gtagctggcg caacctactc ctggaccggt ccgaacagct tcacgtcatc gcaacaaaat
     2461 cccacgcgtt ccaacgccac cgtcgccgat gcgggcagct actccgttac cgtgaccgtc
     2521 agcggctgca cctccgccgc aggatcaacc agtgtcgtcg tgaacgcgac tcctgcgact
     2581 cctacgatca caccgggcgg cccgacgact ttctgcgccg gcggcagcgt cacactcaca
     2641 tcgagcgcag cgtccggcaa tcagtggtac ctgaacgcag gcctgatcac cggcgcgacg
     2701 ggcaacacgt atagcgccgt cgccagcggg aactacaccg tagtcgtgac caccagcggc
     2761 tgcagcagcg ccccgtcggc ggcaatcagt gtcaccgtca accccgctcc tgccacacca
     2821 accatcacac ccggtgggcc gacgacattc tgcaatggcg gcagcgtcct gctgacctcg
     2881 agcagcgcca ccggctacca gtggttcctc ggcggcaacc cgatcaccgg cgccaccaac
     2941 cagacctaca gcgccaccgc atccggcaat tacaccgtcg tggtctcgaa cggctgcaac
     3001 agcgccccgt cggcgcccac caccgtaacc gtcaatccga ttcccgccac accgaccatc
     3061 agtgccggcg gctccacaac gttctgtgaa ggcggaagcg tgacgctcac atcgagcagc
     3121 gcgacaggca accagtggta ccgcaacggc gtgctgctcg gcagcgaaac gagccagacc
     3181 accgtcgcta cgctcgcagg cgcctacagc gtcaccacga cggcaagcgg ctgcaccagc
     3241 gcccagtcgg cggcgacaat catcaccgtc aacccgaagc cgaacgtgac gatcagcgtc
     3301 ccgtcgccga tgtactccgg tgcaagcggt accgcctcag tgatccatga gtgcaccggt
     3361 gcgaccttcg cctggagcat catcggcggc acgatcacat ccggcaatgg cacgcgcatc
     3421 atcacattca ccgcgggccc ggccggcacg ctcacgctct tcgtcaccgt gacgaattcc
     3481 tttggctgct cggattcgaa gaccgccgac gtcaccgtgc aacaagcacc gttcggtgcg
     3541 ccgccatact tcagcgccgc cgccacaagc acgacgagcg tgagcatgct ctgggcaacc
     3601 gtcgccaccg ccgatcacta cgagatctat cgcagcacag acaacacgaa ctggacgttg
     3661 cgcggaacga acactctcgc cacgttctcg gagtccggtc tgacaccgtc gacgacttac
     3721 ctctacaagg tgcgggctat caaggccgac accaccgcct cgggatactc agccatcgat
     3781 cccgcgacga cgatcgtatt caccgatcca tcgctcatcg cctgcccgac gctcatcaga
     3841 ccaatccaca tcaccgagct tcggactgcc atcaacgcgg cccgcgcaag cgtcggtctg
     3901 acggcattcg cgttcaccga tccgtccctc ggagcgggag cacagatcaa agcagttcac
     3961 gtgaccgagc tgcgcaccgc cctggcccca gtgctcagcg ccatcggagt gacgccaacc
     4021 tacacagacc caaccatcac cgccggaacg acacacgtga aaggcacgca cattcaggaa
     4081 ctgcgcgacc tcatccgcta accgcaactc gttccaatcg agcgccttct cccccgcttc
     4141 tcgaggggga gaaggcacct gagggtcgga ggtcgcgttc tccttccccg aggtcacgac
     4201 ccccttcgtt ctgatctcga tctccagtct cgagatcgcc atccccgaga gatgaacgaa
     4261 cgcggaggag cactagatca gcgcatctgc cgtgttttca agcaattcgc acgtggcttg
     4321 gctgggagtc ggcgtgcgtc gcgcctgccg cgcagtcgga aacggcgatt gcgatatcgg
     4381 aaccgagcat ttcgacatcg gaatgatttg ttccggcatc ggaatgattc gttccgacat
     4441 cggaatgatt cgtttcgaca tcggaatgat tcgtttcgac atcggaatga tttgttccgg
     4501 catcggaatg atttgttccg acatcggaat gatttgttcc gacatcggaa tgatctgttc
     4561 cggcatcgga atgatctgtt ccgacatcgg aacaaaacat tccgacttat taccgccctc
     4621 tcccgtcctt tggcccgcat ttctcacact tgcaccgaat tgccgggcaa cgtgctccca
     4681 atcgagcgtt cgaagccgga tgaggggcgt ttgcctgtcg cgccaacctt cgcaaaaaaa
     4741 ctgggcaagg atgccttttc gatcaggtca cgctcgcaaa gaacgtgttt tgtcccatct
     4801 ttcgacgccc ctcatcccgg tgagttacgg ggggcatgca gccacgagca tcccatcgtt
     4861 cctttggcca gccaggtata gctaggccga gccccgtgcg gaagcaaccg cggccacgta
     4921 ctctctcgct ttttcggtga tgctgctcgc ctcgcctctg cgaagggcgg ccaggtcgac
     4981 gaggtcggcg ccgacgccga gggcgaaagc gccggcggcg atgaaatcgg cggcgttctt
     5041 cagcgacacg ccgcccgtgg ggatcagcgc aatctgcggc agcggggcct tgatcgactt
     5101 cagataggtc ggaccgccga gggcgttggc ggggaagact ttcaccgcat ccgcgccggc
     5161 tttccacgcg gtcacgatct cggtcggagt caatgcaccg gggaagatgg cgacgccgag
     5221 gcggcgacag agctcgatgg tggggatgtc gagcgatgga ctgacgacga agcgggcgcc
     5281 ggcatcaatg caggcttgcg cggactccgc atcgagtacg gtgccggcac cgatcagcgt
     5341 ctcgtcaccg aagcggcccg tgagctccgc gatgatcttc accgcgccgg gcacagtgag
     5401 ggtgatctcg aggatgggaa tcccgccggc gcggatcgcc tcgacggcct cgagcgcctc
     5461 ggcggcggat gtcgctcgca ccaccggaac gatgcccact tcgcgaattc gatcaacggc
     5521 tgacacgagc ggttccacct ttcatcacac gttcgacctc ggccagcgtg gccatgctcg
     5581 tgtcgccggg tgtcgtcatc gccagcgcac cgtgcgccgc gccgcattcg acggaccacg
     5641 cgggcccgcg tcccgtcagg agcccgtaga tgaggcccga ggcaaaggaa tcgccgccgc
     5701 cgacacggtc gagaacttcg aggtcggtgc gggttaaggc ttcgtggaac tcgccatcac
     5761 accaggcgat cgcgccccag tcgttgcgtg tagcggtgtg tgccacgcgc aaggtggtgg
     5821 ccacaacctg aagagcgggg aactcggcgc acacgcgccc gatcatctgc ctgaacgcgt
     5881 cggcaggcag cgtcgcgtgg tgttcgtcga ccccttcgat gttgaacccg agggcaacgc
     5941 tgaagtcttc ttcattgccg aagagaacgt cgacgaagct gacaagttca cggttgacag
     6001 cacgcgcttt ctctgcgccg ccgatgccct tccaaagcga tggccggaag ttcaagtcgt
     6061 aggaaacaat gacgccgtgc cggcgagccg cttccatcgc ctcgcgggcg accagtggag
     6121 tcgtctccga cagcgccgcg aaaatgccgc cagtgtgaaa ccaacgaaca ccactcgcga
     6181 agagactctc ccaatcgatg tcgcccggct tcagttgcga gatcgcggtg tgcccgcggt
     6241 cggaacaccc catcgcggcc cgcacgccga agccgcgctc cgtgaagttg agaccattgc
     6301 gcgcggaacg gccgacgcca tcgtccgcgg cccatcgcag cagtgagagg tcgcagcctc
     6361 cctgcagcat cagatcttcg acgaggcggc cgaccgggtt atcgacgagc gcggtggcga
     6421 tcgcggtgcg caatccgaaa cagcggcgca gtcctcgggc cacgttgtac tcgccgccgc
     6481 cttcccacac ctgaaaggag cgcgccgttg agacgcggcc atcaccggga tcgagacgaa
     6541 gcatgacttc gccgagactc acgaggtcga agcggcaatc ggcggcgtcg ttgatcttca
     6601 ctttcgcgaa gcctccagcc ccgacccacc ctgtgcatac tccggctgca cgttgacgac
     6661 gccgaactgc cgatcgtccc cctcgcggat ggcggccatc atccagagaa agttgatcgt
     6721 gcctccgggc gcggagacgt tcgggtgata gccctgcggc atgagcacga cgtcgccttc
     6781 acggacgacc gcaaccagtt ccgggttgcg cggatcgttg tacacgagct gcagacccca
     6841 gcctggttcc ggcatatcga tgtagaggta ggcctcctcc agcatgcgcg cgtgctcgtg
     6901 cggcggccat gaagtccaat tgccgggatg actgaaggtg accccggcca ggatccggcc
     6961 ggcatcgatg ttcttcccga tgacgatgtt gacgtcacgc tccgatgaag ggccgccggt
     7021 atgaaagtga agactggcgt cgttctgcac gtcggcgaat cgaaccagtt ggatgggata
     7081 acgattgtcc accggcgcgg cgagctccgc gacatcgcaa ttgcccgaaa cggtcacatc
     7141 gctgtcgcgc ggaacataga gcgcgtcata gcgcgcgagg tcgtatgtcg ttccgccggc
     7201 tgcgatcgtc gccgtccctt tcagacagat gaatgcagtt tcaagctctc cggtcgagaa
     7261 ggtcaccgca gcgtccgacg cttcgagcac gatgcggcca aagtgaagat ggcgcgcgga
     7321 cgtcgtttcc ggcgtgacgg tgatgtgccg cccccgtctc gcgccggtgt cgcgtacaac
     7381 gcacgtccgc ggctcgatgg ccgccaggtc gactgtcatg gtttcctctt ctcaggtttc
     7441 ggtgcaggcg cggtttccac tgtgacgtcg gtgagtgtca gatcgacctc tccctcgatc
     7501 acgtgtccgt tcgcgacgcc gcggaatgcg gaatcgcgaa cgacgactcc tcgaagcggc
     7561 gtcttgtcga aggcgcgaat gtaaaggccg tactggctgt gcatggagtg cattcgctcg
     7621 acgacgatat tacgcacggt cgggacgaac gtaccggtct cggacatgta ccgcaggtcg
     7681 atctcgatcg gagcgaacat caaggttccg atctcgatgt cacgcgcgaa gatgttttcg
     7741 acaatccccc cgcgagcact gttcgttttg atgcgaagcc cgcgctcgag gatcggactg
     7801 tcgagccggt tcttttcggc atagatgttc cgcgcgccgc cgctgatctc gcttccgacg
     7861 gtgatgccgc cgtggccacc gttcatacgg catccgcgga tcaccacgtt ttcgatcggt
     7921 acgttgaggc ggcggccgtc ggcgttgcgg cccgatttca gggcgatgca atcgtcgccg
     7981 gtgtcgaaga cgcaatcctc gatgagaacg tcgcggcatg attccggatc gcagccgtcc
     8041 atattcgggc cgtgcccgaa caccgtcacg cgacggatcg taacgttcgt gcagagtgtc
     8101 ggatgcaaga cccagaacgg cgcattgcgg atggtgacgc cttcgatgag gatgttcgtg
     8161 cagcgaagcg gctcgacgaa tgtcggccgc aggaagtgac cagagccata gacgcgctgt
     8221 gcgaccggaa ccgcttcgtc gttttgcttg aacagggcgt tccgatcggc gctctgaagc
     8281 cgcggctcct tctccccttt ccagttccac caatgcgttt catcggcctg gccgtcgagc
     8341 gttccctcgc cggtgatggc gacgtcttcg caatcaaggg cggagatcag cggcgcgtag
     8401 ttcatcaact cgacgccttc ccatcgggtg agcgtgagcg ggtagcgcga cggatcctga
     8461 ctgaagcgga gggtcgcgcc ggcatcgatg tgcagatcga cgtggctttg cagacgaatc
     8521 ggcccggtga tgtagtcgcc cttcggaacg atgacatgac caccgccggc gtgcgcacat
     8581 gcatcgatcg ctttggcgat ggccacgctg gcgtcagcac cgtcgtgcgc gccgaacttc
     8641 gtgatgacga agtcgcgaga ggggaagctt ggcggaacga tacgggcgag aattccgggg
     8701 actgctgccc agggatcggt tgtctccgca gttgctgaaa cgctgacgag aagcgtgctg
     8761 agcgcaagca gggtgcgtaa gagtctggct aactcggtca ttgattatgt ccaggaagca
     8821 cgcaccacag aggcgcagag agcaccgagg tcctctatgt cctctgtgtc tctgtggtga
     8881 atcaagaaag agccaatttg cacgttagcg accgtccacg acctcaaccc gcaacggtga
     8941 cttcactcgt tgcgcgaact gctcgacgta cttgtcgaaa tcgctgacat ccgcaaaatc
     9001 gccgctcttg tcccagccgg tgcctgcata gtagacggct ggtgtctttg ccacgatcag
     9061 gtgattacca tccgcctcgg tcacgtcgac gatcgcggcg ggatcgatga cgatggcgca
     9121 accgaatttg ccggtaccgc caacgaccgg tccccatgcg cgaatccatc ccgcggagcg
     9181 ctccatgcgc acatcagtcg cggcgaattt cttgatgcct gttgcgtaag tagggtcgtt
     9241 gacaccgtcg acgacgtagc ggctctcgaa ccggttgaag ttctgtccgg cgtcaagtgt
     9301 gatgcgcttg gtctcggacc gcacgccggc aacgtcccag atcggatagt caagctcgaa
     9361 gaccagccga atcgggccat tcgccaggac gcgcgagttg cgaaagtttt tcgagacgat
     9421 cagcttgccg tcgcgccaga gaccactgcc cccgcagccc cggcttcggc cggccgaata
     9481 gaagtcggcc ccttcgccgg tatcgcggtg atagtcgtcg accatgtacc agtcgttgac
     9541 gatgaggcgg cgcgttcgct tgagccagac gtcgacggtg ctcgacgtca gcggctcttt
     9601 ctgccacgtc tccagagccg tgccgtacat ccggtgggca acgcgatcgt tctcccaggc
     9661 gaagtcgtcg taccgctcgc ggacgaaccg tccgtaaacg tggaagtctt ccttcttgta
     9721 ggtcacccgc tcgccagttc gaagatcgaa cgatcgcgcg cccatgggtt tcatgtcggt
     9781 ctggaagatc aggctctcct gtttgccgtc tccgtcgaga tcgagtgctt gcgccagaac
     9841 ctcgcgtccc gtgtcgtgat cgaagacgtg gacgcgggtc aggtcgccgc cgagaggaac
     9901 aaacaaggaa gcggcaatct ccacagtctc cgaggagcgg gcgattggca gggcattacg
     9961 gacgatgacg cgcagttcag caggcgccga tgcggcctgc agattgcgag cgcccgtaag
    10021 aaaagcgccg gcggccatga tggccgccgg cagcagatga cgaaggagtc ggggcaacct
    10081 caccttagaa ggatagcc
//
LOCUS       DATLVW010000662         5480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_564001, whole genome shotgun sequence.
ACCESSION   DATLVW010000662 DATLVW010000000
VERSION     DATLVW010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564001"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1430
                     /locus_tag="VNN25_19200"
     CDS             156..1430
                     /locus_tag="VNN25_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH93717.1"
                     /translation="MTPPSARSSRRWTIIALLNAGMIIAYVSRSNLPVALTLPDFIRA
                     FNLSDVDRGTLNSAFFWAYAALQIPAGWVVDRYGVKWPYALSLVFWCLASAGTVFARS
                     VTQLTALRVILGVGESIVAPASYKWIRFNFAEKERGLAIGLYMAGTKIGPAIAPPLAA
                     WLIMQYEWRTMFLILGLGGLVWLVPWIVLVQNDAQRPAKDALEGAAVAPIRFGRLMAS
                     PVVWGTVIATFCYMYFVYFCMTWMPAYFVEQRHQSLARMGWYTFYSFAGMAVMAALGG
                     WAADWMIRRGGNPVTVRKWFTIAGFVIACTELIGARASSLTMAVTFAIVSLSGLGLAT
                     ANYWAITQTLFPAGAIGRMTGVQNCAASVAGIVAPIATGWLKQQTGSYEAPMNAIWIV
                     LVVGILSYLIMVREKYAPEGRREPPGMLGQRR"
     gene            1557..2321
                     /locus_tag="VNN25_19205"
     CDS             1557..2321
                     /locus_tag="VNN25_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXH93718.1"
                     /translation="MIDELGLRNYSEVTAHAYIGAAERFAKYFGRSPEQLGPEQIRDF
                     LLHLLHNKATANTIQLYRSALKFLYGKTLKRPWFDEEVARIKKRPQLPTVLSAEEITR
                     ILDRTTNLKYWTLFATLYATGLRCNEVRHLQVGDIDSQRMLIHVREGKGRIPRDIGLS
                     PVLLERLRVYWRWRKPKDWLFPSQQYPAQPLDTATIRRACRIAGRRAGITKRVSPHVF
                     RHASAYYTTFRKQFIPKRIAPGQWQFAEVYGHAVPR"
     gene            complement(2251..4320)
                     /locus_tag="VNN25_19210"
     CDS             complement(2251..4320)
                     /locus_tag="VNN25_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXH93719.1"
                     /translation="MNEKIKPHHLERGAYVYVRQSTPYQVRNHVEGKERQYALAERSK
                     QLGFEKVIVIDEDLGRSGDGTQERAGFGRLLACVCQGLAGAVLALEASRLARNNRDWH
                     HLVDLCALTETLLVDSDGIYDPQQLNDRLLLGLKGTMSEFEVGLLRQRAREAFEQKVK
                     RGFALWELPVGFVRTEEGGIEKTPDRQVQQTIASVFQKFQQLGSARQATLWFRDEQIP
                     VPRVKPGTAGREVVWALPSSGRIRQMLRNPCYAGAFAYGKTAARTVIQEGRARHGSRY
                     RKPQSEWKVLIVDHHPGYISWEEYVENRRRLEANVVADSEGSGAAKLGSALLSGLLRC
                     GRCGRKLQVLYSGNGGRVPRYVCHGDRGDRDSSRCLTVGSLRLDRAVVHSVLQAIQPM
                     GIEAAIRMSERTQTEDDDKRKALELALERARYEASRARRQFDAVEPENRLVASELEVR
                     WNSALARVAEIETRLATVGQQAEPLGKEQEKELMALGEDLTALWNHPEAPAPLKKRIL
                     RTVLTEIVVDSDADPSAHRLHLHWAGGVHTQLRVEHNKTGQHRHSADRTVIDLVSELA
                     KVCPDKTIAAILNRLGYKTGHEKTWNASRVAGIRWYHEIAPFQKQDGWVTQEQAAREL
                     AVSNTVVKRLIRERILPANHVVECAPWVIEREHLALPEVQRQVNAVRHGRRLPQTATG
                     QERFSLE"
     gene            4766..5002
                     /locus_tag="VNN25_19215"
     CDS             4766..5002
                     /locus_tag="VNN25_19215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXH93720.1"
                     /translation="MSADHEHYEKAFRHACATHMLDAGTDLRTIQVLLGHADIRTTAR
                     YLRVSLQRLQGAHSPFDALSLKPVERGKDDGRRR"
     gene            4999..>5480
                     /locus_tag="VNN25_19220"
     CDS             4999..>5480
                     /locus_tag="VNN25_19220"
                     /inference="COORDINATES: protein motif:HMM:NF025677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HXH93721.1"
                     /translation="MTEPRLEVADVFRQHGQEFLDRWGEVLSRQQRKALRDISACRTA
                     ALGTHLEQCDHCAHQVIAYQSCRNRHCPKCHSRTRDEWLAQRAEEILPVPYCHVVFTL
                     PRELAAVALQNPRVSYGILFRAAAESLLELAADPKRLGARLGFLAVLHTWSQRLQCHP
                     "
BASE COUNT         1024 a         1742 c         1541 g         1173 t
ORIGIN      
        1 gcaatgaagg cgttgcggca agcgggcttc agcggcgccg ccgagccgga tcacgtgccc
       61 aagctcgccg gcgataccgg cttgttgcgc gcgggcgccg cgtactgcat cgcttatatg
      121 cgagcgctat tgcagcgggt aaatgaggaa gctgggtgac tccgcccagc gctcggtcca
      181 gccggcgctg gaccatcatc gcgctgttga acgccggcat gatcattgcc tacgtctcgc
      241 gttccaacct gccggtggcc ctgacgctgc cggatttcat ccgagcgttc aacctgtcgg
      301 acgtggaccg cggcactctc aactctgcat ttttctgggc gtatgcggcg ctgcaaattc
      361 cggccggatg ggtcgtcgac cgttacggcg tcaagtggcc gtatgctttg agtctcgtgt
      421 tctggtgcct ggcatccgca ggtacagtat ttgcccgatc cgtcacgcag ctaaccgctc
      481 tgcgggtgat ccttggcgtc ggagaatcga tcgtggcgcc ggccagctac aaatggatcc
      541 ggttcaactt cgcggaaaag gaacgcggcc tggccatcgg gctttacatg gcaggcacga
      601 agatcgggcc tgcgattgcg cccccgctcg ccgcgtggtt aatcatgcaa tacgagtggc
      661 gcacgatgtt cctgattctc gggctgggcg gcctggtctg gctggttccc tggatcgtgc
      721 tcgtccaaaa cgatgcccag cggccggcga aggatgcgtt ggaaggtgcc gcggtagccc
      781 cgattcgatt tggccggttg atggccagtc ccgtggtctg gggtaccgtc atcgccacgt
      841 tctgctacat gtactttgtg tacttctgca tgacctggat gcccgcgtac tttgtcgagc
      901 agcggcacca gtcgctcgcg agaatgggat ggtatacgtt ctacagcttt gccggtatgg
      961 cggttatggc tgcacttggc ggatgggccg cagactggat gatccggcgg ggcggaaacc
     1021 cggttaccgt gcggaagtgg ttcacgattg cgggcttcgt gatcgcttgc accgaactca
     1081 tcggcgctcg cgcgtcttct ttaacgatgg cggtcacttt cgccatcgtt tcgctctcgg
     1141 gcctggggct cgctaccgct aattattggg ccatcacaca gacgctgttt cccgctggtg
     1201 ccatcgggcg catgacgggc gtgcaaaact gcgcggcgag cgtggcgggt atcgttgcac
     1261 ccatcgccac tggctggctc aagcagcaga ccggcagtta cgaagctccc atgaacgcga
     1321 tctggatcgt attggtggtc ggcatcctct cttacttgat catggtgcga gagaagtacg
     1381 cacccgaggg ccgacgcgag ccgcctggaa tgttgggtca gcgaagatag tgtccgataa
     1441 ggcggaatga acaaagccgc tgaagcggca gggcataccc cgtggcgttt tcggtggtga
     1501 aggagtaaac caacgatcga aaggagttcg tcggcttgac ttcactacga aaacgaatga
     1561 tcgacgagtt gggtctccgg aattattccg aggtcacggc tcacgcatac atcggcgctg
     1621 cagagcggtt tgccaagtat ttcggcaggt caccggaaca gttagggcct gagcagattc
     1681 gtgatttcct gctccacctg ctccacaaca aagccaccgc aaacacgatt cagctctacc
     1741 gttcggccct caagttcctg tacggaaaga cgctcaagcg accgtggttc gacgaggagg
     1801 tggccagaat caagaagcgg cctcagctcc ccaccgtgct cagcgcagag gagatcacgc
     1861 gcatcctgga tcgaacgacc aacctcaagt actggaccct tttcgcaaca ctgtatgcga
     1921 caggtttacg gtgcaatgag gttcgacatc tccaggtcgg cgacatcgac agtcagcgga
     1981 tgctgatcca tgtccgggag ggcaagggcc gaattccgcg cgacatcgga ttgtctccgg
     2041 tcctgctgga acgtcttcgc gtctactggc gctggcgtaa gccaaaggac tggctttttc
     2101 catcccaaca gtatcccgcg caaccgctcg atacagcgac gatccgacgc gcctgtcgga
     2161 ttgccggacg gcgagcgggc attaccaaac gggtgagccc ccatgtattc cggcacgctt
     2221 cggcatacta caccaccttt cggaaacagt tcattccaaa gagaatcgct cctggccagt
     2281 ggcagtttgc ggaagtctac ggccatgccg taccgcgttg acttgccgct gcacctccgg
     2341 caaagccaga tgttcccgct cgattaccca cggagcacat tcgaccacat ggttcgccgg
     2401 cagaatgcgt tcgcggatga gccgcttcac cacggtattg ctaaccgcca attctcgagc
     2461 cgcttgctct tgcgtgaccc agccatcttg cttctggaag ggagcgattt cgtggtacca
     2521 gcgaatccct gccacgcgag acgcgttcca cgtcttctcg tggccggtct tgtatcccag
     2581 gcgattcaga atcgctgcga tggtcttatc cggacatacc tttgctaact cacttactaa
     2641 gtcgatcaca gtgcggtctg cactatgccg atgctgtccc gtcttgttat gctccactcg
     2701 cagttgcgta tgaacgccac cagcccagtg gaggtgaagt cgatgtgcag atggatccgc
     2761 gtcactgtcg acgacgatct ccgtcagcac ggttcgtagg atccgtttct tcaacggtgc
     2821 cggcgcctcg ggatgattcc acagtgcggt cagatcctcg cctagcgcca tcaactcctt
     2881 ctcttgctcc ttgccaagcg gctccgcctg ttgcccaaca gtagccagcc gcgtctctat
     2941 ttcagccacc cgcgccagcg cgctgttcca gcgaacctcc agttcgctcg ccaccagccg
     3001 attctcgggc tccaccgcat caaactgccg acgcgctctg ctcgcctcgt aacgggctcg
     3061 ctctaatgcc agttccagcg ctttccgctt gtcgtcatcc tcggtctggg tacgctcgct
     3121 cattctgatc gcagcctcga tccccatcgg ttgaatcgcc tgcagaacgc tgtggaccac
     3181 ggctctatcc aagcgcaggc tccctaccgt caggcacctg ctcgaatcgc ggtctcctcg
     3241 atcgccatgg caaacgtacc gtggtacccg gccgccgttg ccgctgtaaa gcacctgcag
     3301 cttccttccg caacggccgc aacgcagcaa tcccgataac aaagcagagc caagttttgc
     3361 cgcaccactc ccctcactat ctgctaccac gttggcctcc agccgtcttc ggttctccac
     3421 gtactcctcc caactgatgt aacccggatg atggtcaaca atcaatacct tccactcact
     3481 ttgcggcttc cgatagcgag agccgtgtcg ggctcgcccc tcttggatga ccgtgcgagc
     3541 ggcggtcttg ccgtaggcaa acgcgcccgc ataacacggg tttctcagca tctgccggat
     3601 tctcccgctg ctcggcagcg cccagacaac ctctcgcccg gctgttccag gcttcactcg
     3661 gggcacggga atctgctcat cccgaaacca gagggttgct tgtctggcgc tccccagttg
     3721 ctgaaatttc tggaatacgc tggcgatcgt ctgttgtacc tggcgatccg gcgtcttctc
     3781 gatcccgccc tcttcggtgc gaacgaaccc gactggtagt tcccacaacg cgaatccgcg
     3841 ctttaccttt tgctcgaacg cctcgcgggc acgttgtcgc aatagaccca cctcaaactc
     3901 cgacatagtg cccttcagcc cgagcagcag acgatcgttg agttgctgtg gatcatagat
     3961 gccgtcgctg tctacgagca gcgtttccgt gagcgcgcac agatctacca gatgatgcca
     4021 gtctcgattg ttccgcgcca gtcgcgatgc ctctagtgcc agcacggctc cggctagacc
     4081 ctgacagacg catgccaaga gccgaccgaa gcccgctcgt tcttgtgtcc cgtcgccgga
     4141 tcggcccaag tcctcgtcga tgacaatcac cttctcaaaa cccaactgct tggatcgttc
     4201 cgccagggcg tactgacgct ccttgccttc cacgtgattg cgaacctgat acggcgtcga
     4261 ctggcgcacg tagacgtacg ctccgcgctc caagtggtgt ggcttaatct tctcgttcat
     4321 tctgcctccg ttcgtctttg tggagaacgc ttgccagtag ttgggtgcac aggcttcgac
     4381 agcgttcccg cacctcttcc ggaagagcgg cccaaagcgc ctcgtcctcg aagcgaaatc
     4441 tccgctgcca agtgctcggg ctctcgttgt tgtgcatgct gacctcctgt tgggtattct
     4501 gcacaactac ggaaatccgg tgtggagatg agcggctgac tatcgatcag ctggcgcaac
     4561 tccaccagag caccaatcgc gactcgcggt tcggcttcgt cactcaagcg ctcgcatgcc
     4621 tgcggactca acatccattc gggtaccgcc agctgtgaac catcgggaag tctcacaatc
     4681 agaattacag agctgatcct ccgcaagtgg cgaatcagct cgacctctac gccatagaac
     4741 gggtgatggc gatagcagat cttcgctgag tgcggatcat gagcattacg agaaggcctt
     4801 tcgccatgct tgcgccaccc acatgctgga tgctggcacg gacctccgca ccatccaggt
     4861 tttgctgggc cacgccgata ttcgaaccac tgcccggtat ctccgcgtat ccctgcagcg
     4921 gctgcaaggc gcccacagtc cattcgatgc gctctctctg aagcctgtcg agcgtggcaa
     4981 ggatgacggc cgacggcggt gaccgagcct cggctggaag tcgccgatgt cttccgccaa
     5041 cacggtcagg agtttctgga tcgctgggga gaagtactgt ctcgccagca acggaaggca
     5101 ctccgagaca tcagcgcatg ccgtaccgct gcgctcggca cacacctgga gcaatgtgac
     5161 cactgcgcac accaggtcat cgcataccaa tcctgtcgca atcggcattg tccaaagtgc
     5221 cacagccgga cgcgcgacga gtggctcgcc cagagggcgg aggagatcct gccggttccc
     5281 tattgtcatg tcgtattcac gctgccgcga gaattagcgg cggtggcatt gcagaaccca
     5341 cgcgtcagct acggaattct gtttcgcgcg gcagccgagt ccctgctcga attagcggcc
     5401 gatccgaaac gactgggagc caggctgggc ttcctggccg tcctgcacac ctggagccaa
     5461 cggctccaat gccatcccca
//
LOCUS       DATLVW010000663         5850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_481450, whole genome shotgun sequence.
ACCESSION   DATLVW010000663 DATLVW010000000
VERSION     DATLVW010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481450"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1162
                     /locus_tag="VNN25_19225"
     CDS             104..1162
                     /locus_tag="VNN25_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93722.1"
                     /translation="MTKKNIVHVVGTGTIGEPLIGLFTDFKEKWGIDEVTFHKRTPKS
                     DDRAMVEHLINHGGRLVVDEDARDEFTRLGHRVSFTTEQALERATVIVDCTPAGNDNK
                     AKYYDRCTGPKGFLAQGSEFGFGKPYARGINEEVQSPDERFVQIVSCNTHNISVLLKT
                     IAIRDGKIDLEAGRFVCMRRANDVSDNKGFVAAPEAGKHDDPEFGTHHARDAYHLFQT
                     IGEKLNLFSSAIKINTQYMHTLWFDLDLRHDTTLEQVKSLLRSNPRVACTNKRSVNQI
                     FSFGRDHGYFGRILNQTVMPMSTLTVPNPRKVVGFCYTPQDGNSLLSSIAATLRFLDV
                     SKLEENLDVLRPYIFREI"
     gene            1162..1611
                     /locus_tag="VNN25_19230"
     CDS             1162..1611
                     /locus_tag="VNN25_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93723.1"
                     /translation="MDVRRPSRETLRTVVTFVAAAALAVALVRRYHALGGPYLEFPKT
                     VQDHVAPTPYPSRDVILLAGAAAEIIPRGSTVTAIQPSQAPDYDVTHYLTAAGMMPRY
                     RVVPPAFTASDPAALPQYVLAVREPFTNPHYRLFRQIPEGRIYEVVH"
     gene            1608..2897
                     /locus_tag="VNN25_19235"
     CDS             1608..2897
                     /locus_tag="VNN25_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93724.1"
                     /translation="MKDVLAFCAYLLLPLIGIGIWRLPSIRRFDLGGRLAAAGAAGAA
                     IVAALLALLSIASIRWSRGVVFPVVAVIAAIGIYLARPLKRSDAPAERRDPWAIAALA
                     VAAILVVYGLLTARMTCGDLHFFWGPKAVHFYRDGGVTVSFLGDPNAARMNPGYPLLL
                     PLLYAWSETIAGQFLWWSAVLSTALFLFGSVALLRSTARDSRGAVLMCATLAYALAVA
                     DAAGAGEPPLIFFETLAIVAVTFIREPRDQTILAGLGLAGAVMTKIEGATFVIAVVIA
                     MLILRRSPKRTLFIALPAAVLLGAWLGFVRFHDLSEYYRGAAMPIYFAALPGTLLTLA
                     KAAGYELYGLPWLAPIILFVLGPNRRNGILPMTIAVLTFMAAVFFYIHLPDPTWWILS
                     SAPRVLLTPLTALLIASAAAWRSNGDLSYSPGHGVVS"
     gene            2881..3741
                     /gene="accD"
                     /locus_tag="VNN25_19240"
     CDS             2881..3741
                     /gene="accD"
                     /locus_tag="VNN25_19240"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXH93725.1"
                     /translation="MAWFRKEKKPKEAVEKVAIPEGLWVKCDDCKEIVYRKEVEQNFN
                     VCPKCNYHFRLSARERFEILFDDGKYKEFAGDIRSGDPLEFRDTKRYRDRLKVYELRV
                     GKGDAVLCAEGKLDGISVVICAMEYAFMGGSMGSVVGEKITIAAERALEKKEPFLIVS
                     TSGGARMQEGILSLMQMAKISAALARLAEARLPYVSILTDPTTGGVTASYAMLGDLNI
                     AEPKALIGFAGPRVIEQTIRQTLPEGFQRAEFLVEHGMVDLITPRSEMKPALARFLRM
                     FTGKKREAAS"
     gene            3874..5094
                     /locus_tag="VNN25_19245"
     CDS             3874..5094
                     /locus_tag="VNN25_19245"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXH93726.1"
                     /translation="MNAGSLLWLDALQASGIRPGLGRMRAFLRALRHPERAYPSIIVA
                     GTNGKGSTSATLASILNTSGYRSDLYTSPHLVRLHERWTIGGVEINDESLVGAIEKLR
                     SAAGEVGITPTYFEALTLLAFIAFADAKCEIAVLEVGMGGRLDATNVVRPIAALITPV
                     GLDHTEFLGTTIRKIAAEKAGVIHRGAIVLTSNDDPIVLDVIRKRAAKFGSPFHHVTE
                     THATPLAGAFQERNAALAVRAAEELRPVLPKITTESIERGVAATRWRGRLERLERGGT
                     QIWIDGGHNAHAIRAILPFLAERIPAPRLLVFGIMNDKDVADVAALLFPLFEAVIATE
                     PYPPRSVQAADLVARAQRMSVDATAEPDPSRAIARALASGYRSIVIAGSLYLAGTAID
                     FFDAHGDEQRSEQK"
     gene            complement(4990..>5850)
                     /locus_tag="VNN25_19250"
     CDS             complement(4990..>5850)
                     /locus_tag="VNN25_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93727.1"
                     /translation="EKFALVLCAALVVLAAEYFGRSATPRIWAIVTFVPLLCWMVWMI
                     PIDWYSPYRTIAQPTRRVFVPPAPGGQSIDRDSYRERARRLEPLFGATAGLEYVLNRS
                     GDGMHSLLSRIAAERFAATHNSAWLRLAVTPSAIVPEAIAAPSIPDAVNLIESGAPAV
                     APRAFSSAPGARVTRAQREGQQIKIDVASSGPSLLAVDQSYFRAWSARIGDRELETLP
                     VNLDRLGVIVPGSGTVILRFGRHHTLVVVAWVLSSLLLAALLVAMRIEKVDGSSGEVE
                     RAGDDDRAVA"
BASE COUNT         1064 a         1897 c         1805 g         1084 t
ORIGIN      
        1 tatatcaaga gtcacgctag ccataccgaa aggatgcatc cttccggaat cgccgtcaac
       61 ttaccctgtt acaatccgcg cgcttttccg gggagcgcaa acaatgacga aaaagaacat
      121 cgtccacgtc gtgggaacgg gcacgatcgg cgagccgctg atcggattgt tcacggattt
      181 caaggaaaag tggggcatcg acgaggtcac gttccataag cgcacgccga agtccgacga
      241 ccgggccatg gtcgagcacc tcatcaacca cggcggacga ctcgtcgtcg acgaggatgc
      301 ccgcgacgag ttcacccgcc tcgggcaccg cgtttccttc accaccgagc aggccctcga
      361 gcgggccacc gtcatcgtcg actgcacgcc tgcgggcaat gacaacaaag cgaagtacta
      421 cgaccgctgc accggtccga aaggctttct ggcccagggc tcggagttcg gcttcggcaa
      481 gccgtacgcg cgcggcatca acgaggaagt gcaatcgccg gacgagcgct ttgtccagat
      541 cgtgtcgtgc aatacgcaca acatctccgt gctgctcaag acgatcgcca tacgcgacgg
      601 aaagatcgac ctggaagcgg gccgcttcgt ctgcatgcgc cgcgcgaacg atgtgtcgga
      661 caacaagggg tttgtggccg cccccgaagc gggcaagcac gacgatccgg agttcggcac
      721 ccatcacgcc cgggacgcct accacctctt ccagaccatc ggcgagaagc tgaacctctt
      781 ctcgtccgcc atcaaaatca acacccagta catgcacacc ctgtggttcg acctcgatct
      841 gcgtcacgac acgacgctcg aacaggtcaa gtcgctgctg cgctcgaatc cgcgcgtggc
      901 ctgcacgaac aagcggtccg tcaaccagat cttctcgttc ggacgcgatc acggctactt
      961 cggccgcatc ctcaaccaga ccgtcatgcc gatgtcgacg ctgacggtgc cgaatccgcg
     1021 gaaggtcgtc ggtttctgct acacgccgca ggatggcaac tcccttctct cgtcgatcgc
     1081 ggccacgctg cggtttctcg acgtttcgaa gctggaggag aatctcgacg ttctgcgccc
     1141 gtacatcttc agggaaatct aatggacgtt cggcggccgt ctcgcgagac gctgcgcacg
     1201 gtcgtcacct tcgtcgcagc cgcggcgctc gccgtagccc tcgtacggcg ctaccacgcg
     1261 ctgggcggac cgtacttgga gtttccgaag accgttcagg atcacgttgc gccaacgcct
     1321 taccctagcc gcgatgtgat tctgctggcg ggcgcggccg cggagatcat tccccgcggc
     1381 tctaccgtca cggcgattca gccttcgcaa gcgcccgatt acgacgtcac ccactacctc
     1441 accgccgccg ggatgatgcc gcgctaccgg gtcgtaccgc cagccttcac ggcgagcgat
     1501 ccggccgcgc tcccgcagta cgtcctcgcg gtccgcgaac cgttcacgaa tccgcactat
     1561 cggctcttcc ggcagatacc ggagggacgc atttacgagg tcgtgcattg aaggacgtgc
     1621 tcgcgttctg cgcgtatctc ctgttgccgc tcatcggcat cggaatctgg cgcctcccat
     1681 cgatccgccg cttcgacctc ggcggccgtc ttgccgccgc cggcgctgcc ggtgccgcaa
     1741 tcgtggccgc gcttctcgct ttgttgtcga ttgcatcgat tcgttggagc cgcggggtcg
     1801 tgttccccgt cgttgcggtg atcgccgcaa tcggcattta tctcgcccgg ccgctgaaac
     1861 gctcggacgc gccagcggaa cgaagagatc cctgggcgat tgcagcgctg gcagtcgccg
     1921 cgatcctcgt cgtctacggc cttctgacgg cgcgcatgac ctgcggcgat ctccatttct
     1981 tctggggacc aaaggccgtt cacttttacc gcgacggcgg cgtgaccgtc agtttcctag
     2041 gcgatccaaa cgctgcgcgg atgaaccccg gctatcccct gctgctgccg ctgctctacg
     2101 cctggtccga gacgattgcg ggccagttct tgtggtggtc ggcggtcctg tcgacggcgc
     2161 tgtttctgtt cggcagtgtt gcgctcctgc gcagcaccgc acgcgatagc cgaggcgccg
     2221 ttctgatgtg cgcaactctc gcgtatgcac tcgccgtcgc agacgctgcc ggcgcaggcg
     2281 agccgcccct catcttcttc gagacgctgg ccatcgttgc cgtgaccttc atccgcgagc
     2341 cgcgggacca gaccatcctc gctggactcg gcctcgccgg ggcggtgatg acgaagatcg
     2401 aaggcgcgac gttcgtgatc gctgtcgtaa tcgcgatgct gatcctgcga cggagcccga
     2461 agcggactct tttcatcgca ttaccggctg cagtgctgct cggggcatgg ctcggcttcg
     2521 tgaggttcca cgacctctcc gagtactacc gtggcgcggc catgcctatc tattttgcag
     2581 cgcttccggg cacgcttctc acactcgcga aagcggccgg ttacgagctc tacggccttc
     2641 cgtggctggc gccgatcatc cttttcgtcc tcggcccgaa ccggcgaaac ggcatccttc
     2701 cgatgaccat tgccgtgctg acgttcatgg ccgcggtctt cttttacatc cacctgcccg
     2761 atccgacgtg gtggatcctc tcctccgcgc cgcgcgtgtt gctgacgccg ctgacggcac
     2821 tgctgatcgc atccgcggcg gcgtggcgtt caaacggcga cttgagctac agtcccggac
     2881 atggcgtggt ttcgtaaaga gaagaagccg aaggaagcgg tcgagaaagt cgcgatcccc
     2941 gaggggctct gggtcaagtg cgacgactgc aaagagatcg tttaccggaa ggaagtggag
     3001 cagaacttca acgtctgccc gaagtgcaat taccacttcc ggctcagcgc tcgcgagcgt
     3061 ttcgagatcc tgttcgacga cggcaagtac aaagaattcg cgggcgacat ccgctccggt
     3121 gacccactcg agtttcgtga cacgaagcgc taccgggacc gcctgaaggt ctacgagttg
     3181 cgcgtcggaa agggagacgc ggttctctgc gcagagggga aactggacgg gatcagcgtc
     3241 gtcatctgcg cgatggagta cgccttcatg ggtggatcga tgggatccgt cgtaggcgag
     3301 aaaatcacga tcgccgccga gcgtgcgctg gagaaaaagg agccgttcct gatcgtctcc
     3361 acgtcgggcg gcgcgcggat gcaggaggga atcctttcgc tgatgcagat ggcgaagatc
     3421 tccgctgccc tggccaggct ggccgaggca cgcctgccct acgtttcgat cctgaccgat
     3481 ccgacgaccg gcggcgttac ggcgtcgtac gcgatgctgg gcgacctcaa cattgccgag
     3541 ccgaaagcac tgatcgggtt cgcaggtccg cgcgtaatcg agcaaaccat ccggcagacg
     3601 cttcccgagg gatttcagcg ggccgagttt ctcgtcgagc acggaatggt cgacctgatc
     3661 acaccccgct cggagatgaa gccggcgctg gcgcggttcc tacgcatgtt cacaggcaag
     3721 aagcgcgaag cggcgtcctg atcggcacga agcgaaggat ctcgccactt tcaaaatggg
     3781 gtcaggtcta aatttgaaag tgaagaatcg actctcgctg cggccagact gcagtctgtc
     3841 actttcaaat ttagacctga ccccattttg aaagtgaatg ccggctccct tctctggctc
     3901 gacgcgcttc aggcgtctgg catccggccc ggtctgggcc gtatgcgcgc gttccttcgc
     3961 gcactgcgcc atccggaacg cgcctacccg tcgatcatcg tcgcgggcac gaacggcaaa
     4021 ggatccacat cggcgacgct ggcctcgatc ctcaacacgt ccggctatcg atcggacctt
     4081 tacacctcac ctcatctcgt gaggcttcac gagcgatgga cgatcggtgg agtggagatc
     4141 aatgacgaat cgctggtagg agcaatcgaa aagttgcgat cagccgcagg cgaggtgggc
     4201 atcacgccta catacttcga agcgttgact ctcctcgcgt tcatcgcgtt cgccgacgcc
     4261 aagtgcgaga tcgcggtcct cgaggtcgga atgggggggc ggctcgatgc cacgaatgtc
     4321 gttcgtccga tcgccgccct gatcaccccg gtcggcctcg atcacactga gttcctcggc
     4381 accacgattc gcaagatcgc cgcggagaaa gcgggcgtca tccatcgcgg cgcgatcgtg
     4441 ctcacgtcga acgatgatcc gatcgttctc gatgtcatcc gcaaacgcgc tgcgaagttc
     4501 ggcagcccgt ttcatcatgt gacggagacc catgcaacac ctctggcggg tgcctttcag
     4561 gagcgcaatg ccgcacttgc tgttcgggcg gccgaggagt tgcggccggt tctgccgaag
     4621 atcacgacag agtcgatcga gcgtggcgtc gccgcaacgc gctggcgcgg acggctcgag
     4681 agactcgagc gcggcggcac gcagatctgg atcgatggcg gacacaacgc ccacgccatc
     4741 cgcgcaatcc tgccgttcct cgcggaacgg attcctgctc ctcgtctgct cgtcttcggc
     4801 atcatgaacg acaaggatgt cgccgatgtg gcggcacttc tgtttccgct cttcgaggcg
     4861 gtcatcgcca ccgagccgta tccgccgcgg tccgtgcagg ccgccgatct cgttgcgcgg
     4921 gcacagcgca tgtcggtcga tgcgactgcc gagccggacc cctcgcgggc aatcgcacgt
     4981 gcgctcgcgt caggctaccg ctcgatcgtc atcgccggct cgctctacct cgccggaact
     5041 gccatcgact ttttcgatgc gcatggcgac gagcaacgca gcgagcagaa gtgacgagag
     5101 cacccaggcc accacgacga gcgtgtggtg ccggccgaag cgcaggatca cggtgccact
     5161 cccgggaacg atgacgccga ggcggtcgag gttgaccgga agggtctcga gctcgcggtc
     5221 gccgatgcgg gcgctccacg cgcggaagta cgactgatcg acagcgagca gcgacggacc
     5281 gctggacgcg acatcgatct taatttgctg cccttcgcgc tgcgcgcgcg tcacgcgtgc
     5341 gccgggcgcg gaggagaacg cgcgcggcgc gaccgcaggc gcaccgctct cgatgaggtt
     5401 cactgcatca ggaatcgatg gcgccgcgat ggcttcgggg acgatcgcgg agggcgtcac
     5461 tgcaagacgc agccaggcag aattgtgggt ggccgcgaag cgctcagcgg cgatgcggct
     5521 gagtagagag tgcatgccat cgccggagcg gttcaagacg tactcaagcc ccgcggttgc
     5581 tccgaacaac ggttccaacc ggcgggcgcg ctcgcggtaa ctgtcgcgat cgatcgactg
     5641 tcctccgggt gccggcggta cgaacacgcg acgcgtgggc tgcgcgatgg tgcgatacgg
     5701 cgagtaccaa tcgatcggga tcatccagac catccagcac agtagcggca cgaacgtcac
     5761 gatggcccag atccgcggcg tggccgatct tccgaagtat tcggcggcga gaacaaccag
     5821 ggcggcgcag aggacgagtg cgaatttctc
//
LOCUS       DATLVW010000664         5696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_439039, whole genome shotgun sequence.
ACCESSION   DATLVW010000664 DATLVW010000000
VERSION     DATLVW010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439039"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="VNN25_19255"
     CDS             <1..1649
                     /locus_tag="VNN25_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93728.1"
                     /translation="HTGKVSVVGQALNQNLVNLVYDPLVPQEMGANGGELLAHYQSPL
                     VDGNNVYMMYKAGTYGNSNYNSEKWGETKYTWQGGTLVTQWQFASDWDAPGSAKDFWE
                     PVFHPALANNFLYVPGGGGSIFKLNKATGAVVTRINPFPTDDGGTRSNIYTASPITVD
                     ASGNLYYNAMQFHDATNIFNNDVIDSWLVKVTPGDVITTVSYSVLTAGAPAATDLCVN
                     AFPSNADGTIVNGPWPPSPTAVPGSVPCGTQRPGINIAPAVASDGTIYTVTRAHFISR
                     EAFLVAVNPNFTQKWIRTLRNRFNDGCNVPVSAGGTLPPNGSPGGCRAGANFGVDPAT
                     NAPGGGRVLDDSSASPVIASDGSILYGAYTRYNYAQGHLMHFAANGNYLGAYKFGWDV
                     TPGIYGSSIVTKDNHYGLVGSYCNDPAFCPDDNSDRGTRYPEAYFVTQLDNNLNVQWQ
                     YQNTNTQSCTRQPDGSVTCVSDHPAGFEWCVNAFVIDANGNVYANSEDGNLFKIPQGA
                     VGVTRIFQQLALGAAYTPTSMDSSGRIYSQNAGHLFVAGN"
     gene            1827..2561
                     /locus_tag="VNN25_19260"
     CDS             1827..2561
                     /locus_tag="VNN25_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93729.1"
                     /translation="MTSAPGSRATALLRAFLSLSVVLLVAVNVHAQSERQVRPNGPGT
                     GIPDDDRVPWKFVEKGAPIEKSSLTLYWLPASQKEMERSELLGSRALIQAGLRCVGFR
                     IVDAGDAASLKLLDATGKLPTALLVNNQGVVVRRLENVRGSLPPNAVERMVHDELAAR
                     DEAMYRAMSDARKHATAGEKDAAITLYKSVWDDRCFFPLAGLEAQRGLKTLGVEVHEV
                     PSTIAADPQLKQPSLNTKPKTEQQKH"
     gene            complement(2612..3385)
                     /locus_tag="VNN25_19265"
     CDS             complement(2612..3385)
                     /locus_tag="VNN25_19265"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXH93730.1"
                     /translation="MRLADKVALITGGGSGIGKASSLLFAKEGAKVVVVDLKLDTAEA
                     TADEIRKAGGDAKAFAADVSKAKDAEAMVRFAEESYGKLNVVFNNAGVFHPDDDSVTN
                     TSEAIWDMVINVNLKGVFLGCQYAVPALLRAGGGSIINTASFVAIMGAAAPQIAYTAS
                     KGGVLAMTREIAVEFARKNIRANSLCPGPVETPLLAKLLSDPARRNRRLVHIPMGRFA
                     RAEEMANAALFLASDESSFVTGTSFLVDGGISAAYITPE"
     gene            complement(3699..5078)
                     /locus_tag="VNN25_19270"
     CDS             complement(3699..5078)
                     /locus_tag="VNN25_19270"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXH93731.1"
                     /translation="MQGRIDTDILKRLVADDEIDTVIAAFPDMYGRLVGKRITGRFFI
                     DEVLRHGMHACDYLLACDMDMDPVPGYAFTSWAKGYGDFRPIPDFNTLRIASWLPKTA
                     LVLCDVYQEKTHELVPVAPRSMLRHQLKRAAEMGFSTMAASELELYVFKDSYETVAEK
                     GFINATPIGRVIEDYHILQGTKEEHVIGAIRSHLERSGVPVESSKGEWGPGQQEIGLR
                     YAEALEMADRHSIYKHAAKEIAWQQGHAITFMAKWDERYAGSSCHIHMSLWDETGKKP
                     QFAGPESLGSIQCSSTFRWFLGGWMKHIRELFAFFAPYPSSYKRYVAGSFAPTGIAWS
                     FDNRTAGFRIVGHGPSLRIECRAPGADANVYLAFAAAIAAGLDGVANHIEPPPAFEGD
                     VYKAASDLPHMPHSLNESIAALAESAWARDVFGENVVDHYLHFFRTEQRKFDAVVTDW
                     ERRRYFEMA"
     gene            complement(5342..>5696)
                     /locus_tag="VNN25_19275"
     CDS             complement(5342..>5696)
                     /locus_tag="VNN25_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXH93732.1"
                     /translation="YLDPTVFVDVKPEMKIAREEIFGPVVAVMPFDSEEDGVRMANDS
                     IYGLSGSIWTRDGSRALRVARAFETGNISINSGSSVHLEAPFGGVKQSGIGRELGLAA
                     LDAYTEWKTVYIDVS"
BASE COUNT         1116 a         1847 c         1695 g         1038 t
ORIGIN      
        1 agcacacggg caaggtctcg gtggtcggtc aggctctgaa ccaaaacctc gtcaacctcg
       61 tgtatgaccc gctggttccc caggagatgg gggcaaatgg tggggaactg ctggcccact
      121 accaatcgcc gctcgtcgac ggaaacaacg tctacatgat gtacaaagcc gggacgtacg
      181 ggaacagcaa ctacaactcg gagaaatggg gcgagacgaa gtacacgtgg cagggcggaa
      241 cgctggtcac gcagtggcag ttcgcttccg actgggatgc tcccggcagc gccaaggact
      301 tctgggagcc ggtcttccat ccggcgctgg cgaacaactt cctctacgtg ccgggaggcg
      361 gaggctcgat cttcaagctc aacaaggcca caggcgccgt cgtcacgcgc atcaacccct
      421 tcccgacgga tgatggcgga acgaggagca acatctacac ggccagtccg atcacggtcg
      481 atgcctcagg caatctctac tacaacgcca tgcagttcca cgacgcgacg aatatcttca
      541 acaacgacgt gatcgactcg tggctcgtga aggtcactcc cggcgacgtc attacgacgg
      601 tcagctattc ggtgctgacg gcaggcgctc cggcggcaac ggacctctgc gtgaacgcgt
      661 tcccctcgaa tgcggatggg acgatcgtca acggtccgtg gccgcccagc ccgaccgcgg
      721 tgccgggaag cgtgccctgc ggcacccagc gtccgggcat caacatcgcc ccggcagtgg
      781 cgtccgacgg cacgatctac accgtcacgc gcgcgcactt catcagccgt gaggcctttc
      841 tcgttgcagt gaatccgaac ttcacccaga agtggattcg gacgctccgt aaccgcttca
      901 atgacggatg caacgttccg gtcagcgccg gcggcacgct tccgccgaac ggttcgccgg
      961 gcggatgccg cgccggcgcg aatttcggcg tcgatcccgc cacgaacgcg ccgggaggtg
     1021 gacgcgtgct cgatgactcg tcggcaagcc cggtgatcgc gtccgacggc agcatcctct
     1081 acggcgccta cacccgctac aactacgcgc agggccacct gatgcacttc gccgccaacg
     1141 gcaactacct cggtgcctac aagttcggct gggacgtcac gcccggcatc tatggcagct
     1201 cgatcgtcac gaaggacaat cactacgggc tcgtcggctc ctactgcaac gacccggcct
     1261 tctgccccga tgacaacagc gaccgcggga cgcgctatcc cgaggcgtat ttcgtcacgc
     1321 agctcgacaa caacctgaat gttcagtggc agtaccagaa cacgaacacg cagagctgca
     1381 cgcgccagcc ggatggctcg gtgacctgcg tcagcgatca tcccgctggg ttcgagtggt
     1441 gcgtaaacgc cttcgtcatc gacgccaacg gcaacgtgta cgccaacagc gaagacggca
     1501 acctcttcaa gattccgcag ggcgcagtcg gcgtgaccag gatcttccag cagctcgcgc
     1561 tgggagccgc ctacactccg acgtcgatgg actcgagcgg caggatctac tcgcagaacg
     1621 ccggccacct gttcgtcgcc ggtaactgat ctttcggcat catccggtcg ctgatcgctc
     1681 gcaacgggcg gtcagcgacc ggactcctcc gcgcctccgc gttaaaccgg gaagtttgca
     1741 actgcgctac ccggttcctg tcgcacggtt gccgtgattg cggacaatca cgggaaacat
     1801 tccgactcgg aaggcgtcaa acaaacatga cctctgctcc aggctccagg gctaccgctc
     1861 tcctccgcgc gttcctctct ctttccgtcg tactcctcgt cgccgtcaac gtccacgcgc
     1921 agtcggaacg gcaagtccgt ccgaacggtc caggaactgg catccccgac gatgaccgcg
     1981 tcccttggaa gttcgtcgag aaaggcgccc cgatcgagaa atcatccttg acgctctact
     2041 ggctgccggc gtcgcagaaa gagatggagc gctcggagct gctgggctcg cgcgcgctga
     2101 tccaggccgg cctccgctgc gtcggcttcc gaatcgtcga tgctggcgac gcggcaagtc
     2161 tcaagctcct cgacgcaacc ggaaagctac cgacagcgct cctcgtcaac aatcagggag
     2221 tggtcgtccg ccgcctcgag aacgttcgtg gctcgcttcc tcccaacgcc gtcgagcgta
     2281 tggtgcacga cgagctggcc gcccgcgacg aagcgatgta ccgcgcgatg agcgacgccc
     2341 gcaagcacgc cacggcaggc gagaaggacg ctgcgatcac gctgtacaaa tccgtctggg
     2401 acgaccgctg cttctttccg ctggccggcc tcgaagcgca acgcggattg aagaccctcg
     2461 gcgtcgaagt gcacgaggtc ccctcaacga tcgccgccga tccgcagctc aagcagccat
     2521 cgctcaacac gaaaccaaag actgaacaac agaaacactg agagcacaga gaaacctcca
     2581 tgtcctccgt gtctctgtgg ttcaaaactc gtcactcagg cgtgatgtaa gccgccgaga
     2641 tcccgccgtc gacgaggaac gacgttccgg tgacgaagga cgactcatcg ctggcgagga
     2701 acagcgccgc gttcgccatc tcctcggcac gcgcaaagcg tcccatcggg atgtgcacga
     2761 ggcggcgatt ccggcgggcg ggatcggaga gaagcttcgc cagcaacggc gtctcgaccg
     2821 gccccgggca gagcgagttc gcgcggatgt tcttccgggc gaattcgacg gcgatctcgc
     2881 gcgtcatcgc cagcacgccg cccttgctcg ccgtgtaggc aatctgcggt gcggcggcgc
     2941 ccatgatggc gacgaatgag gcggtgttga tgatcgagcc gccgccggcg cggagcagcg
     3001 cagggacggc gtactggcat ccgaggaaaa cgcctttgag attgacgttg atgaccatgt
     3061 cccagatcgc ctcggacgta ttggtcaccg agtcgtcgtc gggatgaaag acgcccgcat
     3121 tgttgaagac aacgttcagc tttccgtagc tctcctccgc aaagcgcacc atcgcctcgg
     3181 cgtctttcgc tttggagaca tcggctgcaa acgccttcgc gtcgccccca gctttgcgaa
     3241 tctcatccgc agtcgcctcg gcggtatcga gtttcaaatc gacaacgacc accttcgcac
     3301 cttcttttgc gaagaggagc gaggaggctt tgccgattcc ggaaccgccg ccggtgatca
     3361 gagctacttt gtctgcaaga cgcattctta aggctccttg gaacagaaat gttggatgtt
     3421 gagtgtcgag cgctgagtgg ttaatgacct tgccgcctat cattcactgg caattccggg
     3481 tgtcatcctg agccgccgaa gccgccgcaa gattcgccgc tcttgcaccg gcaggcttta
     3541 ggcggcggag gacggtcgaa ggatctcaaa atgcgacgcg tctggcatgt gcgtcgtaat
     3601 ttgagatcct tcgccgtcct ccgccttcga gcctgccaat agcaagagca attagttgag
     3661 gacggctccg gctgtctcag gatgacacca accaaccttc acgccatttc aaaataccga
     3721 cgcctctccc aatcagtgac caccgcgtcg aacttcctct gttcggtgcg gaagaagtgg
     3781 aggtagtgat cgacgacgtt ttcgccgaat acgtcacgcg cccaagcgct ctccgccagc
     3841 gctgcgatcg actcgttcag tgagtgcggc atgtgcggca agtcggacgc cgctttgtag
     3901 acatcgcctt cgaacgccgg cggcggctcg atgtgattgg cgaccccgtc tagccccgcg
     3961 gcgatggctg cggcaaaggc gaggtagaca ttcgcgtcgg cgccaggagc acggcattcg
     4021 attcgtagcg acgggccgtg accgacgata cgaaatcccg ccgtccgatt gtcgaagctc
     4081 caggcaatac cggtcggcgc aaacgagccg gccacataac gcttgtacga cgacggatac
     4141 ggcgcaaaga acgcgaacag ctcgcggatg tgcttcatcc atccgccgag gaaccagcgg
     4201 aaggtcgaag agcattgaat ggagccgagg gactcggggc cggcgaactg cggcttcttt
     4261 ccggtctcgt cccacagcga catgtggata tggcaggacg acccggcgta acgctcgtcc
     4321 cacttggcca tgaacgtgat ggcgtggccc tgctgccagg cgatctcttt cgcggcatgc
     4381 ttgtaaatcg agtgccggtc ggccatctcc agcgcctcgg cgtaccgcaa tccgatttcc
     4441 tgctgccccg gcccccactc gcctttcgac gattccaccg gcacgccgga ccgctcgagg
     4501 tgcgatcgaa tggcgccgat cacatgctcc tctttcgtgc cttgcaggat gtggtaatcc
     4561 tcgatcacac gtccgatcgg cgtggcattg atgaagcctt tttcggcaac ggtctcgtag
     4621 gaatccttga agacgtaaag ctccagctcc gacgcggcca tggtcgagaa ccccatctcc
     4681 gcggcgcgtt tgagctgatg acgcaacatg ctgcgcggcg cgaccggaac cagctcgtgc
     4741 gtcttttcct gatagacgtc gcagagcacc agcgccgtct tcggaagcca tgaagcgatg
     4801 cgaagcgtgt tgaagtcggg gatgggccgg aagtcgccgt aacccttcgc ccacgacgtg
     4861 aaggcatagc ccggaaccgg gtccatatcc atgtcgcatg cgaggaggta gtcgcacgcg
     4921 tgcatgccgt gccgcagaac ctcgtcgata aagaaacggc ccgtgatgcg cttgccgacg
     4981 agccgtccgt acatgtctgg aaacgcggcg atgacggtat caatctcgtc gtcagcgacg
     5041 aggcgtttga gtatgtcggt gtcgatgcgg ccttgcatgg ttcctctcgc gggggtcggt
     5101 tgtcgggagt cgggggtcgg gagtcggttg tcgggagtcg ggggtcggag agctgagcat
     5161 attgctcgct ccgactcacc gacaacctgg ctccatccag ctcacgtatt tctccggggg
     5221 tctgggatca ggagtcggga gtcggagatt ttgagcatat cgctccgaca cccgactccc
     5281 gacaagcgac tcccgcgcat attgcctgct ccgacccccg actcccgact cccgacaccc
     5341 ctcagctcac gtcaatgtaa accgtcttcc actccgtgta cgcatcgagc gcggcaagac
     5401 cgagctcgcg tccgatgccg ctttgtttca cccctccgaa cggcgcttcg agatgcacgc
     5461 tgctgccgga gttgatggag atgttgccgg tctcgaaggc acgggccacg cgcagcgcgc
     5521 gcgatccgtc gcgagtccag atcgatcccg atagtccata gatcgaatcg ttcgccatcc
     5581 tcacgccgtc ctcctccgaa tcgaacggca tgacggctac gaccgggccg aagatctcct
     5641 cgcgtgcgat cttcatctcc ggcttcacat cgacgaagac cgtcggatcg aggtac
//
LOCUS       DATLVW010000665         3611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_111254, whole genome shotgun sequence.
ACCESSION   DATLVW010000665 DATLVW010000000
VERSION     DATLVW010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111254"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VNN25_19280"
     CDS             complement(<1..824)
                     /locus_tag="VNN25_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH93733.1"
                     /translation="MRTKLTLCCCCAIGLLFALSVSAADAAAPRVILNASAAAPARYL
                     VVLNDTETVASVADELTARHGGRVSHLFRRSLHGFVVEASESAALGIVNDPRVKYVEQ
                     DSTTSGGSSSMQPVSLATQTAPPWGLDRIDQRDLPLDQRYTYNSTGAGVTAYITDSGI
                     NASHVEFGGRVRTGYNFSATLPPDDCNGHGTHVAGIVGSSLHGVAKSVNLVSVRVLDC
                     ANRGFVTDMVAGIDWAIADHQSGQPAVMNVSIYTDSPSVSFDAAIAAAVSDGITVCV"
     gene            882..1109
                     /locus_tag="VNN25_19285"
     CDS             882..1109
                     /locus_tag="VNN25_19285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH93734.1"
                     /translation="MPASTSTLLAEAKKLSPHDRLELIGEIWDTLEDSDLPVTSEERA
                     ILDSRIADLGANPSAQESSTEARSWLESRRR"
     gene            complement(1204..2526)
                     /locus_tag="VNN25_19290"
     CDS             complement(1204..2526)
                     /locus_tag="VNN25_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93735.1"
                     /translation="MVAAIVTIAICVAAGWPLSVLIDRNARLPLRISTAFLLGGALGG
                     AVLFILSSVQLRWSSVTLPAGMLFVAAIADAFARKRAPFDRARITQPFAPQWKGFLAA
                     GIDLMTAVLAAGYALFATVGPPAEADFITIWGVKAREFWIAGGIDWRFLESPFNEFAH
                     VDYPILLPLIFDVQTVIHGSWDARWLGALQVAFGLAALLAVRAFFEEESGDAVLAAAA
                     TLALACIAFSPWIGMAEGPLVASGTVGLLYVRRGVLGSSSADILRGALFLGIAGSFKN
                     EGVALIAAVAAGLLLTAPRFLARLWPAIAIVTPWLVLRRVHHLQTDLTTGPILDRVTG
                     HLANLKPMFSALATYTTGKPLFWIGIGLALLLALRAAVTRERFLLVALLVQTGFFLAA
                     YLVTPHDVTWHVRWSWERIVSQITLPLGFVAIALLMPLIEGRRGRVEG"
     gene            complement(2529..2987)
                     /locus_tag="VNN25_19295"
     CDS             complement(2529..2987)
                     /locus_tag="VNN25_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93736.1"
                     /translation="MRLAAVVAFALILIGGFEPFYLRIFAIDRAREGAAFAELPFQKI
                     RGLKSFLDGVDAQTPPRARIAIWVPFRQWEGGYGYAYYRASYLLPGKQMVPLLALHED
                     RVDFSSLAQADYLASFGERIVAPGFESCWQDAHGVLLKASPPAAPPATPR"
     gene            3119..3595
                     /locus_tag="VNN25_19300"
     CDS             3119..3595
                     /locus_tag="VNN25_19300"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH93737.1"
                     /translation="MDIDLIEYRQEFRSDFERLNRIWLEQHALLEPVDLEYLREPERL
                     ILSHGGQVFFAMQGQTVVGTCAAIPISATTWELAKLAVDPSARGLGIGRRLCDAVLTY
                     ARAAGATEIVLTSHTALVEAIHLYETLGFRHAPLPADVRYLTANVYMELSCGSGSI"
BASE COUNT          756 a         1176 c         1083 g          596 t
ORIGIN      
        1 acacaaaccg tgatgccgtc gctgactgcc gcggcgatcg ccgcatcgaa actgacagag
       61 ggcgaatcgg tatagatcga tacgttcatc acggcgggct ggccactctg atgatcggcg
      121 atggcccagt cgattcctgc aaccatgtcg gtcacgaatc cgcggttggc gcagtcgagg
      181 actcgtaccg aaacgagatt cacggacttg gcaactccgt gcagcgagct gccgacaatt
      241 ccggcgacgt gtgtcccatg accattacag tcgtccggtg gcagtgtcgc actgaagttg
      301 taacccgtcc gcacgcggcc gccaaattcg acgtgggaag cattgatccc gctgtccgta
      361 atgtaggcag tcacaccggc gccggtggag ttgtacgtgt agcgttgatc gagcggcaga
      421 tcgcgttgat cgatccggtc cagtccccat ggcggcgcgg tttgtgtcgc gagcgatacc
      481 ggctgcatcg acgacgatcc gcccgaggtc gtcgagtcct gctcgacata cttgactcgc
      541 ggatcattga cgattcccag agccgcggac tcggacgctt caacgacaaa accatgaagc
      601 gatctccgga agagatggct cacgcgtccg ccatgccgcg ccgtcagttc atcggcgacg
      661 gaagcgactg tttcagtgtc attgagcacg acgaggtatc tcgccggagc cgccgcgctg
      721 gcattgagga tgacgcgcgg cgccgcggcg tccgctgccg aaacggataa cgcgaacagc
      781 aatccaatgg cgcaacaaca acagagagtc agcttggttc gcacaaagtg cagattatct
      841 cgtgatcggc ctgctcaagg ccaactcggt atactccgct catgcctgcc tcgacatcca
      901 cgctcctcgc agaagcgaag aagctcagcc cccacgatcg ccttgagctc atcggcgaga
      961 tctgggacac gctcgaggac tcggaccttc ccgttaccag cgaggagcgt gcgatcctgg
     1021 actcccgaat cgcggacctt ggggcgaatc cctcggcaca ggagtcgtcc acggaagcca
     1081 ggtcctggct ggagtcccgc cgccgttgaa caatcaccct ttctcgcgga cttaacggtc
     1141 tcttgtttca cgcggaggcg cggagatgcg gagaaaacct ccgcgtctcc gcgtgaaacg
     1201 gttctaaccc tccaccctcc cgcgccgccc ttcgatcagc ggcatcagca gcgcaatcgc
     1261 gacgaaaccg agcggcagcg tgatctggct gacgatccgc tcccagctcc agcggacgtg
     1321 ccaggtcacg tcgtgcggcg tgacgaggta ggcggccagg aagaagccgg tctgcacgag
     1381 tagagcaaca agcaggaaac gctcgcgcgt gaccgcggcg cgcaacgcca gcaacagcgc
     1441 gaggccgata ccgatccaga acagcggctt tcctgtcgtg tacgtcgcca gcgcggagaa
     1501 catcggcttc aagttcgcga gatgccccgt cacgcgatcg aggatcgggc ccgttgtcag
     1561 gtcggtctgc agatgatgga cgcgtcgaag cacaagccac ggcgtcacga tcgcaatcgc
     1621 aggccataag cgcgcgagaa accgcggcgc cgtcaacagg aggcccgcgg cgaccgcagc
     1681 gatcagcgcg acgccttcgt tcttgaagga tccggcgatg ccgaggaaca gagcgccgcg
     1741 cagaatgtcg gcggagctcg atccgagtac tccgcggcgc acatacagca gcccgacggt
     1801 cccgcttgca accagcggcc cttccgccat tccgatccat ggcgaaaacg cgatgcatgc
     1861 caatgccagt gtggctgcgg cggcaagcac cgcgtcaccg ctctcttcct cgaagaacgc
     1921 ccggactgca agcaacgcgg caagaccgaa cgccacctgc aacgctccga gccagcgcgc
     1981 atcccacgag ccatgaatga ccgtctgcac gtcgaagatc agcggcagca ggatcggata
     2041 atcgacgtgc gcgaattcat tgaagggact ctcgagaaag cgccagtcga ttccgccggc
     2101 gatccagaac tccctcgctt tgactcccca gatagtgatg aaatcggctt ccgcgggcgg
     2161 cccaacggtg gcaaaaagcg cataaccggc agcaagaaca gccgtcatca ggtcgatgcc
     2221 tgccgcgaga aatcccttcc actgcggcgc gaagggttgc gtgatccgag cacgatcaaa
     2281 cggagcgcgt ttgcgcgcga aagcgtctgc aatcgcggcg acgaacagca tcccagcggg
     2341 cagcgtaacg ctcgaccaac ggagttgcac gctggagagg atgaagagca cagctcctcc
     2401 gagcgcgccg ccgaggagaa acgcggtgct gattcggagc ggcaacctgg cgttgcggtc
     2461 gatcaacacc gacagcggcc atcccgcggc aacacagatc gcgatcgtta cgattgccgc
     2521 aaccatcgtc acctcggcgt cgcgggcgga gcagcgggtg gtgatgcctt caacagcact
     2581 ccgtgcgcat cctgccagca gctctcgaat cccggcgcga cgatccgctc cccgaacgac
     2641 gccagataat ctgcctgcgc cagactagaa aagtcgacgc gatcctcatg tagcgcgagg
     2701 agcggcacca tctgcttgcc gggcagcaga tagctggcgc ggtagtacgc gtagccatag
     2761 ccgccctccc actggcgaaa cggaacccag atggcgattc gcgcgcgcgg cggtgtttgc
     2821 gcgtcgacac cgtcgaggaa gctcttcagt ccgcggatct tctggaacgg caattccgcg
     2881 aaggctgctc cctcacgtgc gcgatcgatc gcgaagatcc gcagatagaa cggctcgaac
     2941 ccgccgatca ggatcaacgc aaacgccaca accgccgcga ggcgcattgc gtttcgagag
     3001 gatggaagtt cggactgcac tgcaacaagg atagaaccgt ttcacgcgga ggcgcggaga
     3061 cgcggtgatg ttctccgctc ctccgcgtct ccgcgtgaaa cggttttatg ctcacgccat
     3121 ggacatcgat ctcatcgagt atcgccaaga gtttcgctcc gacttcgaac gtctgaaccg
     3181 gatctggctg gagcaacacg cacttctcga gccggtcgat ctcgaatatc tgcgcgagcc
     3241 cgagcgcctc atcctctccc acggcgggca ggtcttcttc gccatgcagg ggcagacggt
     3301 cgtcggaacg tgcgccgcaa tcccgatctc cgcgacaacc tgggaacttg ccaaactcgc
     3361 cgtcgaccca tctgcccgcg gcctcggcat cggccgacgc ctttgcgatg ccgtgctgac
     3421 gtacgcgcgt gccgcaggcg caaccgagat cgtcctgaca tcgcacacag ctctcgtcga
     3481 ggccatccat ctctacgaga cgctgggatt ccgccacgct cccctgccgg cagacgttcg
     3541 ctacctgacg gcgaacgtgt acatggagct ttcgtgcggt agtgggtcaa tttgaaaaac
     3601 cggtcaccac a
//
LOCUS       DATLVW010000666        11457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_111263, whole genome shotgun sequence.
ACCESSION   DATLVW010000666 DATLVW010000000
VERSION     DATLVW010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111263"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VNN25_19305"
     CDS             <1..1208
                     /locus_tag="VNN25_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93738.1"
                     /translation="FDDALRDPLSWKLADSMAVRREAPPELLAQARAIAAEDRYAREV
                     LLPIVESPIRFSEARVGDDPRFRTLAVIRLLCSIANKTHEQQPQYGLVLADAALSICA
                     QLPEPAKSKSGWFVGTAWKERANALRYLGRFRDAEAALDDAEEAFGSDERPEPFDLAI
                     VAFVRATVCWELERFEDAVTAARFAAETFSVYGDTTRYLSALIVEGAAYYSATRESDA
                     AAIFERVVTLARSGGEAGILARALGNAGNCYARLQDYDKASSYYTEALAVLDELDFPT
                     ESVRLSWRMAALQVARGAFDEGLAGLDQGRLRLIQLGMANDAALATLDLVAALLALDR
                     PDRVPALCREIAVTFSSEGMMRSAKKALAYLSEAVASGQATPEKVRHVRGFLERLTTH
                     PQEEFQQIQ"
     gene            complement(2109..3014)
                     /locus_tag="VNN25_19310"
     CDS             complement(2109..3014)
                     /locus_tag="VNN25_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HXH93739.1"
                     /translation="MNTAALPPLPLAEWKPTCDTLHMWTQIIGKVRLKLAPLLNHWWE
                     VPLYVSARGLTTSAIPYGDLVFDCEFDFVDHQLLIRASNGRTARVALQPRSVADFYKE
                     TMVRLRECGIDVDISPVPCEVENPIRFDEDQEHASYDRDAVERFWHVLMFSDRVLNRF
                     RTGFIGKSSPVHFFWGSFDLAVTRFSGRRAPANPAADMITREAYSHEVSSCGWWPGGG
                     ATEMPAFYAYAAPEPKGFKEAKVEPSEAFYNDAVSFFVLPYDAVREASDPEDSLLRFC
                     QTTYDAAADAGKWDRAALERVGENA"
     gene            3108..4049
                     /locus_tag="VNN25_19315"
     CDS             3108..4049
                     /locus_tag="VNN25_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93740.1"
                     /translation="MNSIKRLAASMTGAVAIAALVGTSAFAESRHSDATDEHHSHERS
                     GRSSDSHDHGSSRSNDHRSGDTSRTQNDTYRNYDRGRTQNDTYRNNDRGRTYGDTYRN
                     ETRNRSNDAYRYNGAVQSETYRNNNRGSFDRGSVNYRNGGSQNYNYRNNRPAYDSRGR
                     RNYSIGGRINRFVHESGGYRIWVDGGRFPIWIPEARIGLFPRLRVGLSLNFGGYYDPL
                     GYVEAYDYYDGASYGYGYGNTYSSGLLRGVVETVDYRRGTLVLRDDTSGSFVTALIRD
                     RRLESLRPGDYAEISGDWTRNGVFEALRLENVDGGRY"
     gene            4361..5062
                     /locus_tag="VNN25_19320"
     CDS             4361..5062
                     /locus_tag="VNN25_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXH93741.1"
                     /translation="MRKVLFVIASLTLFISSAFAAKPVLAVAEFRNDTSAGWWSSDVG
                     SDLAGMLSNELAATEKFKLVERHKIGAVLDEQDLAESGRVKKSTGAKLGQMTGAQYVV
                     IATVSAFDSQTRETGGGLSFHGIGVGGKQEEAYLAVDLRVVDTTSGEIVVTRTVEARS
                     TGGGMGVSVYRGGFGGHLSNYEKTPTGKAIRAVIQEISEYLGCAMVDKDSCLDEYKAK
                     DQSRRAKNKKSIKLD"
     gene            complement(5135..6580)
                     /locus_tag="VNN25_19325"
     CDS             complement(5135..6580)
                     /locus_tag="VNN25_19325"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH93742.1"
                     /translation="MLRISKLFFVALLMLAAPALYAERYEQTINRTLPFHGGRVSIDH
                     AFGAVSVRAGSGTDVHVHAVIRASDSDFGKEIRVTVTESSAGGVAIKTEYPEEHFHVF
                     HTSNSYSVDIDVTMPAAAPLTVHNRFGSVDVSGIHTASEIVNAQGPVVLRDSRGAQRV
                     ESSFGSILVEHSAGDTTVQNANGSVRVVDVDGTLNVTNRFGSVTVNDTHNVTIHNGNG
                     SVELLGVAGNATIVNSFDTVTVGNVAGDLDLNNQNGRVDVHDIKGRAVIKNSFGTTKF
                     LNIGRDLSVTTNNGSIEGSHAGSGATLTASFAPITVRDINGPAHIKNANGNVTATEIR
                     GNVDIDTKFGLARVELIKGSLDVENANGGVTATDVSGGVRVHTSFAPVLLKTIAGPVT
                     VENANGSISVADLRGACAAINLKTSFASIRLAIPHNNGYQVEARTSFGSITTDVPVTM
                     TSKSDNSLRGTIGNGACRMQLETANGGINIE"
     gene            complement(6564..7190)
                     /locus_tag="VNN25_19330"
     CDS             complement(6564..7190)
                     /locus_tag="VNN25_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93743.1"
                     /translation="MKINCNQLNDLLLEGGQLSIEAAARHAEECDACMEKLASWNEIS
                     TTAGDLRTTWNNDMLWPRIERAIREDKRRRRTSVWQIAAGLFLLAALGATAWIAQRRI
                     HANDFDNTILRVAAVDEVERAEQAHIAAINHLEALTASKLDQPSTPLLVSYKDKLMML
                     DDAIAECQSAINKNRQNAYLRTQLLTMYSEKQRTLQDVIREEHHATNQ"
     gene            complement(7267..7875)
                     /locus_tag="VNN25_19335"
     CDS             complement(7267..7875)
                     /locus_tag="VNN25_19335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH93744.1"
                     /translation="MDASLAVDLPGTVSNLPTMPQTRTSEDDLRMARACADGSSEVFA
                     AIYASHGERMKSIAFNHLGNISDAEDAVQETFLKVHRAASSYTGEAAFTTWLYRILIN
                     TCYDLLRKRSRRIREAPIDDVVAGSRAAHAVDDVKRMALRKLLNELPEQRRTVFALFE
                     IEGLSHAEIAAILDITEGNSKWILFSTKKQLQEKWKSEVRDS"
     gene            complement(8073..8954)
                     /locus_tag="VNN25_19340"
     CDS             complement(8073..8954)
                     /locus_tag="VNN25_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXH93745.1"
                     /translation="MPELPDVTVYVESIATRIAGQPLLKLRLGNPFVLRTVDPPPSEL
                     AGKRVASVRRMGKRIVIGLDGDLFIVIHLMIAGRFKWLPAGAKVPGKLGLAAFDFPNG
                     TLLLTEAGSKRRASIHIVRGEEAVRAHDPGGIEPLDISLDDFRAALTRERHTLKRTLT
                     DPHVFSGIGNAYSDEILHRARLSPVQMTTNLSEDEIARLYEATRRTLIDWTERLRAEA
                     AVKFPEKVTAFRPDFAVHGRYGKPCPDCGSPVQHIVYSENETNYCARCQTGGKLLADR
                     ALSRLLHSDWPKSIDEL"
     gene            complement(9034..9759)
                     /locus_tag="VNN25_19345"
     CDS             complement(9034..9759)
                     /locus_tag="VNN25_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93746.1"
                     /translation="MNAAVIMETMRRHFTSIGFITYLILIAMTGLFVATFNTPASIWP
                     ALVTILSVITGAAIIGPEFSTALLQLIVSRPIRRSVYLLSRVTGVLACVAVAATVGVT
                     SEIVARLLLGSAAVPWLPLASAYANSLLVSLLAISLLTMLGSLTSSYFNVAIYASVQV
                     ALSAVETLLGLLRTRPNGLSEFLHRHQQLENGLIALDDLLFPAPPPHPDTGWVLRVAA
                     TAAGALVLACIAFERREVPYGGE"
     gene            complement(9756..10661)
                     /locus_tag="VNN25_19350"
     CDS             complement(9756..10661)
                     /locus_tag="VNN25_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93747.1"
                     /translation="MTSAHQGVHAHGVRKSFHAAGRLHEVLKGIDLDLLPGEVVGLIG
                     PNGAGKTTLMSCLLGFLRPEEGGITFDGLPNNDLTIRRRTGFVPERMNLGRRSTGWQF
                     LRYMARVSGVPRAEIDKRAAGMLERLGLTSAKHANLREYSRGMLQRIAIAQALLHKPD
                     FIFLDEPASALDPNGVLLVRDLINEEKQRGAMVLLNSHQLAEVEKVCDRVLFLHGGLV
                     ARAETLRNADRCVMAIRLLTGSFDASAVERIAGTAPIGDIVIVSSETERGVALIVRQL
                     ITSGADIAEIRPQTADLESIFRGAS"
     gene            10694..11023
                     /locus_tag="VNN25_19355"
     CDS             10694..11023
                     /locus_tag="VNN25_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HXH93748.1"
                     /translation="MRIEGVPFALTDWTAVPETIHPGETGVARRRTVQAGNVRVRMVT
                     HSPGYLADHWCSRGHILLVLEGVLVTEIQNGPSHTLSQGMSYQVSDDVAPHRSSTETG
                     ARLFIVD"
     gene            11113..>11457
                     /locus_tag="VNN25_19360"
     CDS             11113..>11457
                     /locus_tag="VNN25_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93749.1"
                     /translation="MHRTYAFASLLIVLLLCPTLVLADSPKSGLLVLHLDFSDSVRDS
                     LSAGRITTVMLAGSSTEGKRFTVNTTSAMVPVSDVGPGTWTIRTFVKMGGTLYAVSEG
                     MNIDVPPGPRFET"
BASE COUNT         2105 a         3484 c         3636 g         2232 t
ORIGIN      
        1 ctttcgacga cgcccttcgc gatccgctgt cgtggaagct cgccgactcg atggccgtcc
       61 ggcgtgaagc gcctcccgag ctccttgccc aggcgcgcgc gatcgcggcc gaggatcgct
      121 acgcgcgcga agtactcctt ccgatcgtcg agtcgccgat tcgattcagt gaggcgcgcg
      181 ttggcgatga tccgcgcttt cgaacactcg cggtcatccg gctgctgtgt tccatcgcca
      241 acaagacgca cgagcagcag ccgcagtacg ggctggtgct cgcggacgct gccctgagta
      301 tttgcgccca gcttcccgag ccggcgaagt ctaagtcagg gtggtttgtc ggaacggctt
      361 ggaaagagag ggccaacgcc ctccggtacc tcggtcgttt ccgggacgct gaggcagccc
      421 tcgatgatgc cgaggaggca tttggttcgg atgagcgtcc ggaaccgttc gatctggcga
      481 tcgtggcgtt cgtcagggcg acggtgtgct gggagctgga gcgcttcgag gacgctgtca
      541 ctgcggcgcg tttcgccgcg gaaacgttta gcgtgtatgg cgatacgacg cggtatctat
      601 cggcactcat cgttgaaggt gccgcgtact acagtgccac tcgtgagagt gacgcggccg
      661 ctatttttga acgggtggtg acgcttgcac gctcgggcgg cgaggccggc attctcgcgc
      721 gcgcactcgg aaacgccggg aactgctacg cccggcttca ggactacgac aaggccagta
      781 gttactacac cgaggctctg gcggttcttg acgagctcga tttccctacg gaaagcgtgc
      841 gtttgtcgtg gaggatggct gcccttcagg tcgcgcgggg agccttcgat gaagggttgg
      901 ctggcttgga ccagggccgg ctgcgactca ttcagctcgg catggccaat gacgctgctc
      961 tcgcaactct cgatcttgtc gctgctctcc tcgcgctcga cagacccgat cgagtaccag
     1021 ctctctgccg ggagatcgcg gttacgttct cgtcagaagg gatgatgcga agcgcgaaga
     1081 aggcacttgc ttatctctct gaggcggtcg cttcgggtca ggccacccct gagaaggtac
     1141 ggcatgtgcg agggttcttg gagcgcctga ccacgcatcc gcaagaggaa tttcagcaaa
     1201 ttcaataaaa acactcagga ttccgtgatc cgcgtcacct cgtattcgga aggcgattga
     1261 ctcgtccgtc cgagaacgag gaggatccac atgcggaatc agattcgtag gtatgcgctt
     1321 ggtctcatct ttattgctac gttggccgcg gttccagcaa ctgctgtcac gagggatgac
     1381 agtggagact gcttcttcaa gtatctgaag tgttttatca cccagatctt cgaacaagga
     1441 gatctgtcgg ttccccccgg ctagcttctc acggtcccaa gtccatcccg ctccagcgcc
     1501 gcgccgccct gtgcgacccg accgcacctg ctgtggcgac gctggtcgtc tatgcagatc
     1561 gcagcagatg atctgcggga tacgaggatg gccggactgg cggagcgtag ttcgcagcgc
     1621 gtaccgtcag gttccccgtg caggacggtc cgccctgcgc ggaccagagg ctcgacgtgc
     1681 gggcaaacgg tttgtcgtca gcggtacgga gtgcagcact gaggtgtccg cagcgtgtga
     1741 cacggagaac tctccctgac accactgggc accctccgta taccactggg cattccccgt
     1801 gtcccattgg gacacagacc atgtcccact ggtacaccga ccgtgtccca gtggggtacg
     1861 tactgtgtcc cagtggggta cgcacggtgt cccagtgggg tacgcaccgt gtcccagtag
     1921 ggtacgtacc gtgtcccagt ggggtacgca ccgtgtccca ctgggacatc gaccgtgtac
     1981 cactgggaca cccaccatgt accactggga caccgaccgt gtaccactgg gacaccgacc
     2041 atgtcccact gggacaccca ccgtgcacca ctggggcgat tcggccgccg tcgatgcgac
     2101 acccgcagtc aggcattctc cccgacgcgc tccagcgccg ccctgtccca tttccccgcg
     2161 tcggccgcgg cgtcgtacgt cgtttggcag aatcgcagca gggaatcctc gggatccgag
     2221 gcttcgcgca ctgcgtcgta gggcagaacg aagaaggaga cggcgtcgtt gtagaacgcc
     2281 tccgatggct cgaccttcgc ctctttgaaa cctttgggct cgggcgctgc gtatgcgtag
     2341 aacgcaggca tctccgtcgc gccgccgccc ggccaccatc cgcaactgct cacctcatgc
     2401 gagtacgcct cgcgggtgat catatcggca gccgggtttg ccggtgcccg ccggcctgag
     2461 aatcgcgtca ccgccaggtc gaagcttccc cagaagaaat gcaccgggct gctcttgccg
     2521 atgaaccccg tccgaaagcg attcagcaca cggtccgaga acatcagcac gtgccagaag
     2581 cgctcgacgg cgtcgcggtc atacgatgca tgctcctggt cttcgtcgaa acgaatgggg
     2641 ttttcgacct cgcacggcac gggcgagatg tcgacatcga taccgcactc ccggagccgg
     2701 accatcgtct ccttgtagaa atcggccacc gagcgcggct gtaacgccac gcgtgccgtg
     2761 cgtccgttgc tggcgcggat cagaagttgg tgatcgacga agtcgaactc gcagtcgaaa
     2821 acaaggtcgc cgtacgggat cgcggaggtt gtcagtcccc gcgcgctcac gtagagtggc
     2881 acttcccacc agtgattcag aagtggcgcg agcttcagcc gcacttttcc gataatctgg
     2941 gtccacatgt gcagtgtgtc gcaggttggc ttccattcgg ccagcggtag gggagggagc
     3001 gcggcggtgt tcatgatcat cattatgcat ctgcggtgca cggcgccgat tctggcagac
     3061 ggattgcgat agtagagcgc agcagtcatc ggtcctaagg agacaacatg aacagcataa
     3121 agaggttagc agcaagcatg acgggcgcgg tggcgattgc ggcgctcgta ggaacgtccg
     3181 cttttgcgga atcgcgtcac tcggatgcga cggatgagca tcacagccat gagcgcagcg
     3241 gacggtcgag cgacagtcac gaccacggaa gctcacggtc gaacgatcat cgcagcggag
     3301 acacgagccg cacccagaac gacacctacc gcaactacga tcgcggccgt acgcagaacg
     3361 acacgtaccg gaacaacgac cgcggccgta cctacggcga cacgtatcgc aacgagaccc
     3421 gcaaccgttc gaacgacgcc taccgctaca acggcgcggt gcagtcggag acgtatcgca
     3481 acaacaaccg cggatccttc gatcgcggaa gtgtcaacta ccgcaacggc ggctcgcaga
     3541 actacaacta ccgcaacaac cgtcccgcgt acgacagccg cgggcgccgg aactactcga
     3601 tcggaggccg tatcaaccgc ttcgtccatg agagcggtgg ttaccggatc tgggtcgatg
     3661 gcggccgctt tccgatctgg attcctgagg cgcgcatcgg cctcttcccc cggctgcgcg
     3721 tagggctttc gctgaacttt ggcggctact acgatccact cggatatgtg gaagcctatg
     3781 actactacga cggagccagt tacggctacg gctacggcaa cacgtacagt tccggcctgc
     3841 ttcgcggtgt ggtcgaaacg gtcgactacc gcagaggaac cctggtgctg cgcgacgaca
     3901 cctccgggag cttcgtgacg gcgctcatcc gcgaccgccg cctggaaagc ctgcgtccgg
     3961 gcgattacgc cgagatctcg ggcgactgga cgcgcaacgg cgtgttcgaa gcgcttcgcc
     4021 tcgagaacgt cgacggcggc agatactgat tctttcgcgt accggttttc gggagtcgga
     4081 gcacagtgct ccggctccct ttttttcgcg ggtgtcgggt ttcgggtgtc gggtgtcgga
     4141 gcggcttctt ggatacgttt cctcgtaatt cgaaacgcct tcgttgtccg atcgcgagct
     4201 tccaccccca acaccccact ccccaccccc cgatcacgct tcaactccga cccccgaccc
     4261 ccgacgtctc tccgacactt ccgacccccc gtgaccccca tcacgcttac cgcgccaaac
     4321 tgcggtagag ttcgcggcac acaaaccaca ggagaaaacg atgcgcaaag ttttgttcgt
     4381 gattgcttca cttaccttgt tcatctcgtc ggcctttgcc gccaagccgg tactcgccgt
     4441 cgcggaattc cgaaacgaca cgtcggccgg ctggtggtcg tctgacgtcg gcagcgacct
     4501 ggccggtatg ttgagcaacg agctcgccgc taccgagaag ttcaagctcg tcgagcgcca
     4561 caagatcggc gccgtgctcg acgaacagga tctcgccgaa tccggccgcg tcaagaagtc
     4621 gaccggcgcg aagctcggcc agatgactgg cgcgcagtac gtcgtcatcg ccaccgtcag
     4681 cgccttcgac tcgcagacgc gtgagaccgg cggcggcctg agcttccacg gaatcggcgt
     4741 cggcggaaaa caggaagagg catacctcgc cgtcgacctt cgtgtcgtcg acacgacgag
     4801 cggcgagatc gtcgtgacgc ggaccgtcga ggcccgctcc accggcggtg gtatgggtgt
     4861 cagcgtctat cgcggcggtt tcggcggaca cctttccaac tacgagaaga cccccaccgg
     4921 caaagcgatc cgcgccgtca ttcaggaaat ctccgagtac ctcggctgcg cgatggtcga
     4981 caaagactcc tgcctcgacg agtacaaggc gaaggatcag tcgcgccgcg ccaagaacaa
     5041 gaagtcgatc aagctcgact aaacccgatt catcgatgat gccaaaagcg ccgctgttcg
     5101 cagcggcgct ttttttatcc gttcgatcgg cggctcactc gatgttgatg ccgccgttcg
     5161 ccgtctcgag ctgcatccgg cacgcgccgt tcccgatcgt tcctcgcaga gaattgtccg
     5221 acttcgacgt catcgtcacc ggcacgtcgg tcgtgatcga gccgaaactc gtccgggcct
     5281 cgacctgata accattgttg tgcggaatgg ccagcctgat agacgcgaag ctcgtcttca
     5341 gattgatggc cgcgcaagct ccgcgaagat cggctaccga gatcgagccg ttcgcattct
     5401 ccacggtgac agggccggca atcgtcttca gcagcacggg ggcgaagctc gtgtgcacac
     5461 gcacgccgcc cgaaacatcg gtcgccgtca cgccgccgtt ggcattctcg acgtcgagtg
     5521 agcctttgat cagctccacc cgcgccagtc cgaacttcgt gtcgatgtcg acgttgccgc
     5581 ggatctcggt ggccgtaacg tttccgttcg cgttcttgat gtgagcgggt ccattgatgt
     5641 cgcgcaccgt gatcggcgca aaactcgccg tgagcgtggc gccgctgccg gcgtgcgagc
     5701 cttcgatgct gccgttgttc gtcgtcacgg agaggtcgcg tccgatgttg aggaacttcg
     5761 tcgtgccgaa cgagttcttg atcacagccc gccccttgat atcgtgaacg tcgacgcggc
     5821 cgttctggtt gttcagatcg agatcgccgg cgacgttgcc gacggtgact gtgtcgaaag
     5881 aattgacgat cgtggcgttg ccggcgacgc cgagcagttc gaccgagccg ttgccgttgt
     5941 ggatcgtgac attgtgcgtg tcgttcaccg tgacggagcc gaagcgattc gtcacgttca
     6001 gcgtgccatc gacatcgacg acgcgcaccg agccgttggc gttttgcact gtcgtatctc
     6061 ccgcgctgtg ctcgacgagg atcgacccga atgagctctc cacccgctgc gctcctcggc
     6121 tgtcgcgcag cacgacggga ccttgcgcat tgacaatctc gcttgcggtg tgaattccgg
     6181 agacgtcgac gctcccgaag cggttgtgta ccgtcagcgg tgccgccgcg ggcatcgtca
     6241 catcgatgtc gaccgaatag gaattcgacg tgtggaacac gtgaaagtgc tcctccggat
     6301 actcggtctt gatcgccacg ccgcccgcgg acgattccgt gacggttacg cggatctcct
     6361 tgccgaagtc ggagtcggag gcacggatga cggcgtgaac gtggacgtcg gtcccgctgc
     6421 cggcacggac gctgacggcg ccgaaggcgt ggtcgatcga tacgcgccca ccatggaacg
     6481 gcagcgtgcg gttgatggtc tgttcgtagc gctctgcgta gagagccggt gccgcgagca
     6541 tgagcagcgc gacgaaaaac aacttactga ttcgtagcat ggtgttcctc ccggatgacg
     6601 tcctgcagcg tccgctgttt ttccgagtac atcgtcagca gttgcgtgcg gaggtaagcg
     6661 ttctgtcggt tcttgttgat ggcagactgg cactcggcga tggcgtcgtc gagcatcatc
     6721 agcttgtctt tgtagctgac gagaagaggc gtcgatggtt gatctagttt cgaagcggtg
     6781 agggcttcga ggtggttgat ggcggcgatg tgagcctgtt cggcgcgctc cacctcgtcg
     6841 acggcagcga ctcggaggat cgtgttatcg aaatcgtttg cgtggattcg ccgctgcgcg
     6901 atccaggccg tcgctccgag ggcggccagg agaaacaacc cagcggcgat ctgccacacg
     6961 ctcgtgcgtc tgcgccgctt gtcctcgcgg atggcgcgct cgatccgcgg ccagagcatg
     7021 tcgttgttcc aggtagtccg cagatcgccg gcggttgtgg agatctcatt ccagctcgcc
     7081 agcttttcca tgcatgcgtc gcactcctcg gcatgccgcg cagcggcttc gatcgacagc
     7141 tggccgcctt cgagtaggag atcgttgagt tgattgcagt tgattttcat agttgcggtt
     7201 cgcgcgttgt cggttatcgg ttatcggttg tcggagcctg atctcttgtc tctgttcgct
     7261 aatccattac gagtcgcgaa cctcgctctt ccacttctcc tgcaactgtt tcttcgtcga
     7321 gaagagaatc catttcgagt tcccttctgt gatgtcgagg atggcggcga tctcggcgtg
     7381 gctcagcccc tcgatctcga acagcgcgaa gaccgtgcgg cgctgctcgg gcaattcgtt
     7441 gagcagcttc cgcaaggcca tccgcttgac gtcgtccacc gcgtgcgcgg cgcggctgcc
     7501 ggcgacaacg tcatcgatcg gtgcttcgcg gatccggcgg ctgcgtttcc gcaaaaggtc
     7561 gtagcaggtg ttgatgagga tgcgatagag ccaggtcgtg aatgcggctt cgccggtgta
     7621 actcgacgcc gcccgatgca ctttgagaaa cgtctcctgc accgcatcct cggcgtcaga
     7681 aatgttgccg aggtggttga aagcgatcga cttcatgcgc tcgccgtggc tggcgtagat
     7741 cgccgcaaac acctcggagc taccgtccgc gcaagcccgc gccatgcgaa ggtcgtcctc
     7801 gctggtgcgc gtttgcggca ttgtcggcag gttcgacacg gtccccggca agtctacggc
     7861 gagagaagca tccatcggtc tgaagggata gacgccgggg aagcgggaaa gttagagaga
     7921 ctccatcgag gggtcgtgga tcgacttggc cggcgtatcg tgatcgtcgg agcaaaggca
     7981 accacagaga cacagagaac actgaggggt ctctgtgtcc tttgtgcctc tgtggtgaac
     8041 ggtttttgaa cttgactcac cggcaccggg tgtcacaact catcaatcga cttcggccaa
     8101 tcgctgtgca gaagccgcga tagcgcccga tcggcgagga gctttccgcc ggtttggcat
     8161 cgcgcgcaat agttcgtctc gttttcggag tagacgatgt gctgcactgg agagccgcaa
     8221 tcgggacacg gtttgccgta gcggccatgg acggcgaagt cggggcgaaa cgcggtgact
     8281 ttctcgggaa acttcaccgc ggcctcggcg cgcagacgtt ccgtccaatc gatcagcgtg
     8341 cgccgcgtgg cttcgtagag acgggcgatc tcgtcttcgg agaggtttgt cgtcatctgc
     8401 acgggcgaaa gccgcgcgcg gtggaggatc tcatccgagt acgcattgcc gatgccgctg
     8461 aagacgtgcg gatcggtgag ggttcgcttg agcgtgtgcc gctcgcgagt aagagcggcg
     8521 cggaaatcgt cgagcgaaat gtcgagaggc tcgatcccgc cgggatcatg cgcgcgaacg
     8581 gcctcctcgc cgcgcacgat gtgaatcgat gcgcgcctct tcgatcccgc ctcggtgagc
     8641 agcagcgtcc cgtttggaaa gtcgaacgca gcaagtccaa gtttgccagg aaccttcgcg
     8701 ccggccggca gccatttgaa acggccggca atcatcagat ggatgacgat gaagagatcg
     8761 ccgtcgagac cgatcacgat ccgtttgccc attctgcgga cgctcgcgac gcgcttgcct
     8821 gccaactcgg acggtggcgg atcgacggtg cgaagaacga aggggttgcc gaggcggagc
     8881 ttgagaagag gctgaccggc gatgcgggtc gcgatcgact cgacgtagac agtgacgtcc
     8941 ggcagttcgg gcacggcacg attatccgct gagggcgaag tggccgctat ttcaagttcg
     9001 cagcgatcaa aggcccggcc ccaacgcgcc tatctactcc ccgccgtacg gtacttcgcg
     9061 gcgctcgaac gcgatgcacg ccagcaccag tgccccggcg gcggttgcgg cgacgcggag
     9121 aacccatcca gtatcggggt ggggtggggg ggcggggaaa aggagatcgt cgagggcgat
     9181 gaggccgttt tccagttgtt ggtggcggtg gagaaactcg ctcagtccgt tgggtcttgt
     9241 gcggagcagg cctaacagcg tttctactgc ggagagtgcc acctggacgc tggcgtagat
     9301 ggctacgttg aagtaagagc tggtgaggct gccgagcatg gtcaggagag agatggccag
     9361 aagcgagacc aggagagagt ttgcgtatgc cgaggcaaga ggcagccacg gcaccgcggc
     9421 tgatccgagc agaaggcgcg ccacgatttc ggacgtgact ccgacagtgg cggcgacggc
     9481 gacgcaggcc agcactccgg ttacgcgcga gagtagatag accgatcttc ggatcggccg
     9541 cgacacgatg agttggagca gtgcggtcga gaattccggt ccgatgatcg cggctcccgt
     9601 gatgacagac aggatcgtca cgagcgccgg ccatatggat gcgggggtgt tgaaggtcgc
     9661 cacgaacagg ccggtcatgg caatcaaaat caaataggtg atgaagccga tgctggtgaa
     9721 atgccgccgc atcgtttcca tgatcacggc cgcgttcatg acgcacctcg gaaaatgctc
     9781 tcgaggtccg cggtctgcgg cctgatctcc gcgatgtccg cgccgctggt gatcagttgg
     9841 cgaacgatca gtgcaacgcc gcgctccgtc tcggaggaga cgatcacgat gtcgccgatc
     9901 ggagcggtgc cggcgatccg ttccaccgct gacgcgtcaa acgatccggt cagcagccgg
     9961 atggccatca cgcagcgatc ggcgttgcga agcgtttccg cccgcgccac gaggccgccg
    10021 tgaaggaaca gcacgcggtc gcacaccttc tccacttccg cgagctggtg agagttgagc
    10081 aagaccatcg ctccgcgctg cttctcctcg ttgatgagat cgcggacgag aaggacgccg
    10141 ttcggatcga gtgcggacgc tggttcgtcg aggaaaatga aatcgggttt gtgcaggagc
    10201 gcctgcgcga tggcgatgcg ctgcagcatt ccgcgcgagt actcgcgcag gttggcgtgc
    10261 ttggcgctcg tgaggccgag gcgttccagc atgccggcgg cgcgcttgtc gatctcggcg
    10321 cgcgggacgc cggagacgcg ggccatgtag cggaggaact gccagccggt gctccggcgc
    10381 ccgaggttca tccgttccgg gacgaagccc gtgcgccggc gaatcgtcag gtcattgttg
    10441 ggcagaccgt cgaatgtaat cccgccttcc tccggacgaa gaaagccgag gaggcaggac
    10501 atgagagtgg tcttgccggc gccgttcggt ccgatcagtc caacgacttc accggggagc
    10561 aggtcgaggt cgattccctt gagtacttcg tgcaggcggc cggcagcgtg aaaggacttg
    10621 cgcacgccgt gagcgtgaac accttgatgt gcggacgtca tcatgcgctt ttcggagacg
    10681 ttagtatgac ggaatgagga tcgagggcgt cccattcgcg cttaccgact ggaccgcggt
    10741 gccggagacc atccatcccg gagagaccgg cgtcgcccgc cggcgaacgg ttcaggccgg
    10801 caatgtgcgt gttcgcatgg tcacgcactc acccggctat ctcgccgatc actggtgctc
    10861 tcgcggtcac atcctccttg tcctcgaagg cgttctcgtg acggagatcc agaacggacc
    10921 cagccacacg ctctcgcaag gcatgtccta ccaggtctcg gacgacgtcg ctccgcatcg
    10981 gtcgtcgacg gaaaccggtg cgcgcctgtt catcgttgat tgaccccgcg gctcggcgga
    11041 gtaaccaaac gttttccttt gcgtctcatt aggtagagag accgccaccg gcactacgca
    11101 aaggagacca agatgcaccg cacctacgct ttcgcctccc ttctgatcgt ccttcttctt
    11161 tgtccaacac tggttctggc cgactcgccg aagagcgggt tgttggtgct gcatctcgat
    11221 ttcagcgact ccgttcgtga cagcctcagc gcagggcgca tcacgacggt gatgctggcc
    11281 ggatcctcaa cggagggaaa gcggttcacg gtcaacacga ccagtgccat ggtcccggtc
    11341 agtgacgtcg gcccgggcac gtggacgata cgtacctttg tgaagatggg cggaacgctg
    11401 tacgcggtct ccgaagggat gaacatcgat gtgccgcccg gcccgcgatt cgagacg
//
LOCUS       DATLVW010000667         2341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_524863, whole genome shotgun sequence.
ACCESSION   DATLVW010000667 DATLVW010000000
VERSION     DATLVW010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524863"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..1126)
                     /locus_tag="VNN25_19365"
     CDS             complement(389..1126)
                     /locus_tag="VNN25_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93750.1"
                     /translation="MKVPAVHRRQFLKLASVAAVGVAFSERRLFAATSSGIVPLLGVG
                     YAASLPQDGFTVSMADASTILSPDPGFISRGARVTVVGASRAKQYANTAGGIGVDAYF
                     PITGREAAKFPRFAFWSAANDALTGQVSFRMPALATTGLAFVVRRLRPAAGDSKSSTV
                     PALETETSPLTLSLGSADGPKLARGVYALAFREENGDSAPSWNRTLLMNQKGQYSLSG
                     VTGVTYVLLQVGYDEEPAASGNHGSKH"
     gene            complement(1240..>2341)
                     /locus_tag="VNN25_19370"
     CDS             complement(1240..>2341)
                     /locus_tag="VNN25_19370"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH93751.1"
                     /translation="SGVELSFESVSASGVAPGTLDEGIVVDRVHGHVTITGVDGKVGT
                     GGAILHARANGIRIAQTANVRITGITITDSGVNVPARGVRCAGTFDVNSTAICRAALY
                     LRHVTDSAFENIVVDGGGAMGINANNLRDVSFSGLDVHRAGDETFESGVLLQELSGAI
                     TFTGSSFSDNAGSEVLVEQRFNSGRLVLDRCTLSAPQRPEVAKQLLSVQVSGGAKLDV
                     AVRNSDLRDSIGSAIEAASSESSSLALSVADSTLQHFGHGIIAGSATQSSHLAIAIHG
                     SQFAAPAVRERAWIEIDSKEKASVCAGVTSNRFTSPEGTAVRLGAAPGSQLAIAGAVS
                     SDNKAIAAAMTAANGGAPIIIAGVTSANLTCP"
BASE COUNT          444 a          724 c          753 g          420 t
ORIGIN      
        1 gccgaggaga ccttcttcga gacggtcgat ggcggattga tcgatcggaa ggtcgggagt
       61 cagcgactcc ttcaccttct cgatgatctc ggtgtgggtc atgaagagcc cacgtttcaa
      121 cttgtcgaaa agcgatttcc cgggttcggc catagggcgc gcattgtagc cggggtcgat
      181 tacgctgcaa ggcagggtcg gccggagcgt gagattcaac taattcgatg ccgctcacgc
      241 gaaggcgcga agacgcgaag cgtttgagga gccgtctttc gcgtcttcgc gtgtatggtg
      301 gagagggagc aagaatcaca aggacagcga gcgccgtcgg agcgcttcaa agctccgaga
      361 cccgaccccc gacctgcgac tcccgccttt agtgtttcga cccgtggttg cctgacgccg
      421 ccggctcttc gtcgtaaccg acctgcagca gaacgtacgt cacgccagtg acaccggaga
      481 gcgagtattg gcccttctga ttcatcagca acgtgcgatt ccagctcgga gcgctgtcgc
      541 cgttctcttc acggaacgcc agcgcgtaga cgccacgagc cagcttcggg ccatccgcgc
      601 tgccaagcga cagcgtcaac ggcgacgtct ccgtctcgag cgccggcacc gtgctcgact
      661 tgctatctcc agcggcggga cgcaagcggc gcacgacgaa cgcaaggccg gtggttgcca
      721 gcgcgggcat ccgaaacgac acttgaccgg tcagcgcatc gttcgcggcg gaccagaatg
      781 caaaccgcgg aaactttgcc gcttcgcggc cggtgatcgg gaagtaagcg tcgacgccga
      841 ttccgccggc ggtgttcgcg tactgtttgg cacgtgaagc accgacgacc gtgacgcgcg
      901 cgccgcggct gatgaagccg ggatcggggc tgaggatcgt cgacgcgtca gccatcgaaa
      961 cggtaaagcc atcctgcggc agcgacgcgg cataaccgac gccgagcagc ggcacgatgc
     1021 cggagctggt ggcggcaaac aagcgtctct cgctgaaggc aactccgact gctgcgacgg
     1081 atgcgagttt gaggaactga cggcggtgga cggctggcac cttcatagct gacttcctgc
     1141 ctaatgttaa gaaatctatt gttttctgtt acttgcgtcg cggcaagctg aacgggagga
     1201 atcctaacac gtttccgttt ccgtgttgtc gcggcgttac taagggcagg ttaaattcgc
     1261 agaggtaacc cctgcgatga tgatcggggc gccgccgttc gcggctgtca ttgctgccgc
     1321 gatagccttg ttgtcggaag acacggcgcc ggcgattgca agctgcgatc cgggcgcggc
     1381 accgaggcga accgccgtcc cttccggcga cgtgaaccga ttcgaggtca cgccggcgca
     1441 gacggatgcc ttctctttgc tatcgatctc gatccaggcg cgctcgcgga cagccggcgc
     1501 ggcgaactgg ctgccatgaa tcgcaatcgc cagatgcgac gactgcgtgg ccgagccggc
     1561 gatgatgccg tgaccgaagt gctggagcgt cgaatcggcg acggagagag ccagcgaact
     1621 gctctccgac gatgccgcct cgatcgcgga gccgatgctg tcgcgcaggt cggagttgcg
     1681 cacggcgacg tcgagcttcg cgcctccgga cacctgcacc gacagaagct gcttcgcaac
     1741 ctcgggacgc tgcggcgcag aaagcgtgca gcggtcgagc acgagccggc cgctattgaa
     1801 gcgttgctcg acgagcacct cgctgcctgc gttgtcggaa aacgatgagc cggtgaacgt
     1861 gatcgctccg ctcaactcct gaagcaacac gccactctcg aaggtttcgt cgccggcgcg
     1921 gtggacgtcc aagcccgaga acgacacatc gcgcagattg ttggcgttta tgcccatggc
     1981 gccgccgccg tcaacgacga tgttctcgaa tgccgaatcc gtcacgtgcc gcaggtagag
     2041 cgccgcgcgg cagattgccg tcgaattgac atcgaacgtt cccgcgcagc gaaccccgcg
     2101 ggccggaaca ttcacgccgc tgtcggtgat cgtgatgccg gtaattcgca cgtttgcggt
     2161 ctgcgcgatg cgaataccgt tggcacgtgc gtgaaggatc gcgccgccgg ttccaacctt
     2221 gccgtcgacc ccagtaatcg tgacgtggcc gtgtacgcga tcgacgacga ttccctcgtc
     2281 gagcgttccc ggcgctacgc ccgaagcgga gacggattcg aacgaaagct ccacgcctga
     2341 a
//
LOCUS       DATLVW010000668         6768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_26553, whole genome shotgun sequence.
ACCESSION   DATLVW010000668 DATLVW010000000
VERSION     DATLVW010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26553"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNN25_19375"
     CDS             <1..480
                     /locus_tag="VNN25_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93752.1"
                     /translation="GANVIFDARDVDDPLQLLLATNYFPPFYTHRPRIAGEAYGDGGL
                     SDNAPYEKALELGCDAVVVVTQKGESEGGLFKAKGDYDHVVTDPRVVVIRPRHRLPFR
                     FTERRWHVLDVLARIGYLRTREVLLGEEHEETNVRAEGEAPSARARRILRAIIRHPV"
     gene            477..2093
                     /locus_tag="VNN25_19380"
     CDS             477..2093
                     /locus_tag="VNN25_19380"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXH93753.1"
                     /translation="MTRALSLTSIALASALFLLAQAPPAAAPPNPANQATVRAANGDH
                     KVTIAQQNGQTYFAADEVIAALGGTLTHEGNGFKASLNNSVAAFGTDSRFAVVKDDLI
                     EMPVPPVTIDNRPFVPWQFFQGFLLRSSGLDATWDPAARLLQIHPAQTTAVGIQVSVA
                     NVQGISKLVLTLTAPAEYAIVKEPGAYTVRFKTAIKAPYVEQAHEDPYVAKTSFIGND
                     LRIQLTAPDVVGDAYRLENPFRIVLDLRKGAAAAPGTPQPVMSSRPVESPGIHTIVID
                     PGHGGKEVGAIGPGGLMEKDATLAVCRLLAASLEARLKTRVVLTRNDDTVVPLDQRTA
                     IANQYHADLFLSVHMNAAIVKGAHGAETYFLSLDASDKLAEKAAEVENNAAKNAAAPA
                     SSSDLKLILWDLAQQEYLNESSRLAQAVQEEMNRITGIQSRGVKQAPFKVLVGATMPA
                     ALVEVAFITNPDEEAKIKSDAFQKTVVEALTTAVARYKVDYETRIGVAQPPPAPAAPT
                     TTAPAPGSMAPTATVPPTTTGPKPATRTGT"
     gene            2093..2620
                     /locus_tag="VNN25_19385"
     CDS             2093..2620
                     /locus_tag="VNN25_19385"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXH93754.1"
                     /translation="MRRSIAILIAILAVVSGCKKRELTQNLNVENKVEVRPVTLFFEG
                     PDMLLVREVRNVPLAENPAAALPVVARELLKGSAKAGVPHIFPRDTMVRAAFLLPDGT
                     AFIDLGGPTLTQGWATGSHEELMAVYSVVQTVTTDFPAAKRVRILINGEPAETLAGHV
                     NLEHALTPMPVFVAK"
     gene            complement(2907..3761)
                     /locus_tag="VNN25_19390"
     CDS             complement(2907..3761)
                     /locus_tag="VNN25_19390"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXH93755.1"
                     /translation="MEHQIRIGPAGWSYSDWEGIVYPPHGSKFDHLAYLASFFDTIEI
                     NSPFYRIPPPSHAKSWVRRVAANPAFKFTTKIFRGFTHDAAGFTENDVKAFRDYLDPL
                     AESNRLGAVLIQYPWSFRNSEESRGRLERLFDGFDGYPLSLEVRHSTFQNDDFLQFLD
                     ERKVSWVNIDQPLFHDSVKPSDTLTGPVGYVRLHGRNFEKWFAHGESWERYNYLYSNE
                     ELTPWVDRIHRMSDAKETYVITNNHFRGQAIVNAVELEASLGMDAKMPPQLKGTYPER
                     ARIGAIPE"
     gene            4075..4398
                     /locus_tag="VNN25_19395"
     CDS             4075..4398
                     /locus_tag="VNN25_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HXH93756.1"
                     /translation="MDTFNDKSLNCADCGQEFVFSKNEQAFYSERGFTNEPKRCKNCR
                     DKRKTARDGNGGGGGRDRQMVSVTCDSCGVGTEVPFTPVSGKPVYCRDCYNQRRTGAR
                     SSSWA"
     gene            4524..5492
                     /gene="cysK"
                     /locus_tag="VNN25_19400"
     CDS             4524..5492
                     /gene="cysK"
                     /locus_tag="VNN25_19400"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXH93757.1"
                     /translation="MKPVRNILDLVGNTPMLQLTNIPGAGMGDLFVKLEFLSPGFSVK
                     DRIGVGMIERAARDGKLAKGGTIIEPTAGNTGIALALAGTRMGYRVILCVPENFSLEK
                     REVMKALGGEVVLTPKDDGMKGAIAKAEELSRSIPDSYVPQQFTNHYNSDAHYATTGP
                     EIWEQMEGRVDGWLAGAGTGGTFSGVARYLKERNPNCWCVVVEPQGSILKGGEAGPHE
                     VEGIGASSFIPPVLDMSLADDVIQIYDDPAFVMVRRLAAEEGVLGASSAGANVAAALQ
                     VAEKLGPGKRIVTLIPDASERYMSKGIYTKWAKVDRESGVGGRESE"
     gene            5704..>6768
                     /locus_tag="VNN25_19405"
     CDS             5704..>6768
                     /locus_tag="VNN25_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXH93758.1"
                     /translation="MAFSTDCIHAGQEPEPVTGSVTYPIFQTSTYVQPELGKNKGFEY
                     ARTKNPTRSVLEANLAALERGKHAHCFASGMSATDTVFRLLKSGYHVIAGENMYGGSY
                     RLFSKVCEKFGVRFTYVDTSNADNVRAALQPETKIVFLETPTNPMMTVTDIAACAEVA
                     HAGGALVVVDNTFCSPYLQRPIELGADLVVHSTTKFLNGHSDSVGGVVISNDDAIAEQ
                     IGFLQNAVGAILSPFDSWLVLRGVKTLAVRMRQHEENGMAMAEYISQHPKALKVYYPG
                     LPDHPQHELAKRQMRGFGSMISFDVGSLANAKKFLDRVRLCSLAESLGGVETLISHPE
                     TMTHASVPLDTRTRLGITPGL"
BASE COUNT         1351 a         2137 c         2045 g         1235 t
ORIGIN      
        1 ggcgccaacg tcatcttcga tgcacgcgat gtcgacgatc cgctccagct ccttctggcc
       61 acgaactatt ttccgccgtt ttacacgcat cggccgcgca tcgccggcga ggcctacggc
      121 gatggcggcc tttccgacaa cgcgccgtac gagaaagcgt tggagctggg atgcgatgct
      181 gtcgtcgtcg tgacccagaa aggcgaatcg gaaggtggcc tcttcaaagc caaaggcgac
      241 tacgaccacg tggttaccga tccgcgcgtc gtcgtcatcc gcccgcgcca ccgccttccc
      301 ttccgattca ccgagcgccg atggcacgtc ctcgacgttc tggcgcggat cggctacctg
      361 cgcacgcgcg aagtcctgct cggcgaagag catgaagaaa cgaatgtgcg cgccgagggg
      421 gaagctccga gcgccagggc gcggcgaatc ctccgtgcga taatccgtca ccccgtatga
      481 cccgtgccct ctctctgacc tcaatcgctc tcgcttccgc gcttttcctt ctcgcgcaag
      541 ctcctcctgc cgcggcgcca ccgaatccgg ccaatcaggc cactgtgcgc gcagcgaatg
      601 gcgatcacaa agtcaccatt gcccagcaga acggccagac ctactttgcc gccgatgagg
      661 tcatcgccgc gcttggcggg actcttactc atgaaggaaa tggcttcaag gcatcgctca
      721 acaactcggt ggcagcgttc gggaccgaca gccgcttcgc cgtcgtcaaa gacgatctga
      781 tcgagatgcc tgttccgccg gtcacgatcg acaatcgtcc attcgtcccc tggcagttct
      841 tccagggctt cctgcttcgc tcctcggggc tcgatgcaac gtgggatcct gccgcgcgtc
      901 tgctgcagat tcatcccgcg cagacgacgg ccgttgggat tcaggtctcc gttgccaatg
      961 tgcagggaat ctcgaagctc gtcctgacgc tcaccgcgcc ggcggagtac gccatcgtca
     1021 aagagccggg cgcgtacacg gtgcgattca agactgccat caaggcgccg tacgtcgagc
     1081 aggcgcacga agatccgtac gtcgcgaaga catcgttcat cggcaacgac cttcgcatcc
     1141 agctcaccgc ccccgacgtc gtcggcgatg cctaccggct cgagaacccg ttccgcatcg
     1201 tcctcgatct tcgcaagggg gcggcagccg ccccaggcac gccgcagccg gtcatgtcgt
     1261 cgcggccggt cgagtcgccc gggatccata cgatcgtcat cgatcccggc cacggcggga
     1321 aagaggttgg cgcgatcggg cccggcggtc tgatggagaa ggatgccacc ctcgccgttt
     1381 gcaggctgct ggcggcttcg ctcgaagcca ggttgaaaac acgcgtggtt ctgacgcgca
     1441 atgacgatac ggtcgtgccg ctcgaccagc gcacggccat cgccaaccag taccacgccg
     1501 atctcttcct ctcggtgcac atgaacgccg ccatcgtcaa aggcgcgcac ggagccgaga
     1561 cctatttcct ctcgctcgat gcctcggaca agctcgcaga gaaggcagcc gaggttgaaa
     1621 acaatgcggc gaagaacgcg gcagcgcccg cctcgtcgag cgatttgaaa ctcatcctct
     1681 gggatctcgc gcagcaggag tacctcaacg aatcgagccg cctcgcccag gcagtgcagg
     1741 aagagatgaa ccggatcacc ggcatccaga gccgcggcgt gaagcaagca ccgttcaagg
     1801 tgctcgtcgg agcgacgatg ccggccgcgc tggtcgaggt tgcgttcatc acgaatcccg
     1861 atgaagaggc gaagatcaag tcggacgcat tccagaaaac ggttgtcgag gctctgacaa
     1921 ccgccgtggc gcggtacaaa gtcgactacg aaacgcggat cggcgtcgcg cagccgccgc
     1981 cggcacctgc cgcaccgacc acaacggctc cggcacctgg ctcgatggcg ccgaccgcca
     2041 ccgtgccgcc aacgacaaca ggcccgaagc cggcgacgag gacgggaacg tagtgcgccg
     2101 gtcgatagcg atcctgattg cgattctcgc cgtcgtcagc ggatgcaaga agcgggagtt
     2161 aacgcagaac ctcaacgtcg aaaacaaggt tgaagttcgt cccgtgacct tgtttttcga
     2221 aggccccgac atgcttctcg tgcgcgaagt gcgcaacgtt cctcttgcgg agaatccggc
     2281 ggcagcgttg ccggtcgtag cccgcgagct tctcaaaggg tcagccaagg ccggtgtgcc
     2341 gcacattttc ccacgcgaca ccatggttcg cgccgccttc ctcctccccg acggcaccgc
     2401 cttcatcgac ctcggcggcc ccacgctgac gcagggatgg gcaaccggca gccacgaaga
     2461 gctcatggcc gtctactccg tcgtgcagac ggtcaccacc gattttcctg cggcgaagcg
     2521 cgttcgcatc ctcatcaacg gagagccggc ggaaaccctc gctggtcatg tgaacctcga
     2581 gcacgcgctg acgccgatgc cggtgttcgt agcgaagtaa ggtagtcgcg tttcccgtgt
     2641 ggcgccaaag gacaacgccc gctacctgaa cagaatcgtg tcatcctgag acagccggag
     2701 ccgccacgcg cactcgaact cttcgcgctg catggtgctg gcggcgtagg acggcgaagg
     2761 atctcaattt acgaggcatg tggcttgtgc atcgcattta gagatccttc gtcgtcctcc
     2821 gccgccagca ccattcggcg caagcggatc gattcgcgcg ggcggctccg gctgtctcag
     2881 gatgacacct aacttaatga gttaagtcat tcggggatgg caccgattct cgccctctcc
     2941 ggatacgttc ccttcaactg cggcggcatc ttcgcgtcca tccccagcga cgcttcgagc
     3001 tcgacggcat tcacgatcgc ctggccgcgg aagtgattgt tcgtgatgac ataggtctct
     3061 ttggcgtcgg acatccggtg aattcggtcg acccacggcg tcagctcttc gtttgagtag
     3121 aggtagttgt agcgctccca cgactcgccg tgcgcaaacc atttttcgaa gttgcggccg
     3181 tgaaggcgca catatccaac gggtcccgtc agcgtgtctg acggtttgac cgaatcgtga
     3241 aagagcggct gatcgatgtt cacccatgac accttgcgct cgtcgagaaa ctgcaggaag
     3301 tcgtcattct gaaacgtgga gtggcgcacc tcgagtgaca gcggatagcc gtcgaagcca
     3361 tcgaacagac gctccaggcg gccgcggctc tcctcgctgt tgcggaagct ccacgggtac
     3421 tggatcaaca ccgcgccgag gcggttggat tcggcgagag ggtcgaggta atcgcgaaag
     3481 gctttgacgt cgttttcggt gaaacccgcg gcgtcgtgcg tgaagccgcg aaagatcttc
     3541 gtcgtgaact tgaacgccgg attggcggcc acgcgacgca cccacgactt cgcgtgcgag
     3601 ggtggcggga ttcggtagaa gggcgaattg atctcgatcg tgtcgaagaa cgaggccagg
     3661 taggcgaggt gatcgaactt cgatccgtgc ggtggataga cgattccttc ccaatcggag
     3721 tagctccatc cagcgggccc gatgcggatc tgatgttcca tgattgatgg tcaaattgat
     3781 ggtcaatacg ttgacgaact tattttgagc aactcgtaat tcgttgcctg cgttgagtat
     3841 ctatgctaaa acgcgagcca acgaactcag cgaaagaacc aaacctcagg actgcggcaa
     3901 agcgttcccg gggtaccgct gtattcgcta ccgaagggag gctggtcgac ctaaccgatt
     3961 cgcttgtgca acacaaaagc ggggagaagg tcgcggcacc ccgcacacat tctgcgctgc
     4021 ggcaccacca tctgatcgta gattcaaagg aacaatttta ggagatcaag gttcatggat
     4081 acattcaacg ataagtctct caactgcgcc gactgtggac aggagttcgt gttcagcaag
     4141 aatgaacagg cgttctattc agagcgcggt ttcacgaacg agccgaagcg ctgcaagaac
     4201 tgccgcgaca aacggaagac ggcgcgcgac ggaaacggcg gcggcggtgg ccgtgaccgc
     4261 cagatggtga gtgtgacatg cgatagctgc ggagtgggaa cggaagtgcc gttcacgccg
     4321 gtcagcggca agcccgtgta ctgccgcgac tgctacaacc agcgccgcac aggcgcccgc
     4381 tcgtcgagct gggcgtaagt tttttcagat cgattacttt cacggcggac cttcgggtcc
     4441 gccgttttgg ttttgggtcg caaccagcag ccccgtgact cagcaacctc gcatacaatc
     4501 ccgcgcgctt gagccggcct cttctgaaac ccgtccgcaa catcctcgat ctcgtcggca
     4561 acacgccgat gctccagctc acgaacatcc ccggcgccgg tatgggggac ctcttcgtga
     4621 agctcgagtt cctcagtccc ggcttttccg tcaaggaccg catcggtgtc gggatgatcg
     4681 agcgcgccgc gcgagatggg aagctcgcca agggcggcac gatcatcgag ccgacggccg
     4741 gcaacaccgg catcgcgctc gcgctggccg gcacccgcat gggctatcgc gtcatcctct
     4801 gcgtccctga aaacttttcg ctcgaaaagc gggaggtgat gaaggcgctg ggcggcgaag
     4861 tcgtactgac gccgaaagac gacgggatga aaggtgcgat cgcgaaggct gaagaattgt
     4921 cgcggtccat ccccgattcg tacgtgccgc agcagttcac gaatcattac aacagcgacg
     4981 cgcattacgc cacgaccggt cctgagatct gggagcagat ggaaggccgc gtcgatggct
     5041 ggctggccgg agccggcaca ggcggcacgt tcagcggcgt ggcgcgatat ctcaaggaac
     5101 gcaacccgaa ctgctggtgc gtcgtcgttg agccgcaggg atcgattctc aaagggggcg
     5161 aagcggggcc gcatgaagtc gaaggcatcg gagcctcctc attcatccct ccggttctcg
     5221 atatgtctct ggccgatgac gtgatccaga tctacgacga tccagcgttc gtcatggtgc
     5281 gccgcctcgc tgccgaggag ggcgtgctgg gcgcatcgtc agcgggagcg aacgtcgcgg
     5341 ccgcgctgca ggttgccgaa aagctcggcc ccggcaagcg cattgtcacg ctcattcccg
     5401 atgcctcgga gcgctacatg tcgaaaggca tctatacgaa gtgggcaaag gtcgatcggg
     5461 agtcgggggt cgggggtcgg gagtcggagt agattcaaaa cccgaccaag gaccggaaac
     5521 gtgagacgac attcggccgg ctccgactcg cgactcccga tcaacgaccc tcgcgaaagg
     5581 aaggcgggtg atcggaagtc ggagtagatt caaaatccga cactaggcac ccacttcgcg
     5641 acaaacgaca ttcgaccgac tccgaccccc gattcccgac aagcgacccc cgaaaggatc
     5701 cccatggcct tctccactga ttgcattcac gccgggcagg agccggagcc ggtcaccggc
     5761 tcggtcacct atccgatctt ccagacctcg acctacgtac agccggagct cggaaagaac
     5821 aaaggtttcg agtacgcgcg cacgaagaac ccgacgcgct cggtgctgga ggccaatctc
     5881 gccgcgctcg agcgtggcaa acacgcccac tgcttcgcct cgggcatgtc ggcgaccgac
     5941 accgttttcc ggctgctgaa gtcgggctat cacgtcatcg ccggcgagaa catgtacggc
     6001 ggctcgtacc gcctgttcag caaagtctgt gagaaattcg gcgtccgctt cacatacgtc
     6061 gacacttcga atgccgataa tgtccgcgcg gcgctccagc cggaaacgaa gatcgtcttt
     6121 ctcgaaacgc cgacgaatcc gatgatgacg gtgacggaca tcgcggcgtg cgccgaggtt
     6181 gcacatgccg gcggcgcgct cgtggtcgtc gacaacacat tctgttcgcc gtaccttcag
     6241 cgcccgatcg agctcggcgc cgacctcgtc gtgcattcga cgacgaagtt ccttaacggc
     6301 cactcggact cggtgggcgg tgtggtcatc tccaacgacg acgcgatcgc ggagcagatc
     6361 ggattcctgc agaacgcggt cggcgcgatt ctctcgccgt tcgattcatg gcttgtcctg
     6421 cgcggcgtga agacgctggc ggtccgaatg aggcagcacg aagaaaatgg gatggcgatg
     6481 gcggagtaca tttcgcagca tccgaaggcg ctgaaggtct actacccggg acttcccgat
     6541 catccgcagc acgagttggc gaagcggcag atgcgcggct tcggctcgat gatttcgttc
     6601 gacgtcggtt cgctcgcgaa tgccaagaag ttcctcgacc gcgttcgtct ctgctcgctc
     6661 gccgaatccc ttggcggcgt cgagacgctg atctcgcatc cggagacgat gacgcacgcc
     6721 tccgtccccc tcgacacccg cacccgcctc ggcatcactc caggcctc
//
LOCUS       DATLVW010000669         7083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_197517, whole genome shotgun sequence.
ACCESSION   DATLVW010000669 DATLVW010000000
VERSION     DATLVW010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197517"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VNN25_19410"
     CDS             <1..247
                     /locus_tag="VNN25_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXH93759.1"
                     /translation="ASLEGVDGVHHLHIWALAPSRPALSCHLMVGDIPLRSTGNLLAQ
                     VNELLEREYGIGHTTVQFEFATCDVDDPYCVPYSEAK"
     gene            complement(721..1284)
                     /locus_tag="VNN25_19415"
                     /pseudo
     CDS             complement(721..1284)
                     /locus_tag="VNN25_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            1444..2121
                     /locus_tag="VNN25_19420"
     CDS             1444..2121
                     /locus_tag="VNN25_19420"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXH93760.1"
                     /translation="MSRKELFEKVALEQIIGVVREESVEAAESVAEAYARNGIRIIEV
                     TLTTPQAFELISRFAQTYAPLDVVIAAGTVRNSNDAAQARRAGAQLIVSPHTDVTVIE
                     YANEHDLLSIAGAGTATEIIRAWEAGCDIVKVFPALYLGGPDYIRTLRGPIRDVPMLA
                     GGPVPLDTIENYLDAGCMAVNLGASLAVPDLVRSEQWEEIGRRALLATSIVQSRNVDV
                     ESDSYVH"
     gene            2155..2898
                     /locus_tag="VNN25_19425"
                     /pseudo
     CDS             2155..2898
                     /locus_tag="VNN25_19425"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3157..3522
                     /locus_tag="VNN25_19430"
     CDS             3157..3522
                     /locus_tag="VNN25_19430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXH93761.1"
                     /translation="MTSRAAAPVGAYPHARRVGDLLFLSGIGPRRPVTNEIPTGIEAQ
                     TRATVDNVRVILEAAGSSLEKILDVTVYLTDMKRDFDSFNRVCAELLGSIQPTRTTVG
                     VDSLPTPIAVEMKIIAAAR"
     gene            complement(3554..4657)
                     /locus_tag="VNN25_19435"
     CDS             complement(3554..4657)
                     /locus_tag="VNN25_19435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH93762.1"
                     /translation="MKVLHLASGNRWTGAAAPAFAEAEALRQAGVDAHYAYVGGYKLQ
                     QKIGHHDFAHPLISKAQNPWSFLRTTDAISRLIEHHRFDVVHAHLTYDHWLTLFAARG
                     SGPVIVRTFHAARVLRSDPFTRWLIARTDGIFVNNDSFAAMEPVRRRSPIFTPPPLDE
                     RQFTPDGPDVRSHYGLGPETLLLMAIGKLSKDRGFEDVLRTYAFVHREIANARLMIIG
                     HGEHRPALEALARELAIDVIWAGYHEDDLAEHYRAADILLFTARGSDEGHRAVLEAMA
                     CGVVPVTFPIAGIPALLGDLSAALIASASTPEAAAEKVAGIANGDIADLRQRAYEQSQ
                     EFSYARAAERLIEAYERLTAFSRLRVSGSRLPL"
     gene            complement(4719..5165)
                     /locus_tag="VNN25_19440"
     CDS             complement(4719..5165)
                     /locus_tag="VNN25_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93763.1"
                     /translation="MSGTRPELSAGLVLRLARRRADELFIENMRSPQAQDFTLGSREL
                     KEAALKRCGQLSVYDREITSAAQARALMTKPGGYPWGFHLSVEAIRGIAFDLHVFRDP
                     NPDGDMREGAEGHCVIENVWSPDEDRMQDIRAELMRIAQPIDEAIS"
     gene            complement(5172..5552)
                     /locus_tag="VNN25_19445"
     CDS             complement(5172..5552)
                     /locus_tag="VNN25_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93764.1"
                     /translation="MSFANIEQTVLSPEEQWLSYLRSLASRKLATPAVAGAAEDLWRG
                     LRASVPAITRPNASPTDEGGLSMSWNLGRHYLEIEIRPSAKYDWFYRDRDLGQSDRQR
                     AQTVESAPSDVLKIHLIAMFEESD"
     gene            5691..5930
                     /locus_tag="VNN25_19450"
     CDS             5691..5930
                     /locus_tag="VNN25_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXH93765.1"
                     /translation="MAIQPQLLEILACPICKEEVRPVPLSEAKRVTVRDKFRDKMRGE
                     ELVVEEGLQCVKCHRVYPIVSDIPVMLVDEAFEEA"
     gene            5940..>7083
                     /locus_tag="VNN25_19455"
     CDS             5940..>7083
                     /locus_tag="VNN25_19455"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXH93766.1"
                     /translation="MFLKRRLDVPEETPLPVALALFFYVGHLFCAGWVASSEVFAFAT
                     TVMVGWCLWRRLLEPSFHILYVPLALYGLASTVSALVAVREIHSFGETALWGKMLLFP
                     AALILLRNVPQARFTALRMVLIFGAMASAMGLAQYVTAGGFRDLEHRITGPTAHVMTL
                     SGLLLPVALTFIVLWIYEAGNLWLLAGAAVTNLALLITFTRSAWLGWIVGIAVLLILK
                     WPKALAWAVPVAIIAINFAPLPLFSRVISSFDTRQSSNLDRIRMAEAGVEIIKDYPVF
                     GVGPANVKEIYPLYRKHDAPRFRIPHLHNNVIQLLAERGVLALLAYLFLQGLFLRECA
                     RAWGGPATKYAEIGVVVVASLGAAGMFEFNFGDTEVFWIMLDLFAIV"
BASE COUNT         1299 a         2198 c         2197 g         1389 t
ORIGIN      
        1 cgcgtcgctg gagggagtcg atggcgtaca tcaccttcac atctgggcgc tggcgccgtc
       61 gcggccggcg ctgtcatgtc acctgatggt tggcgacatc ccgctgcgct cgaccggcaa
      121 tctgctggct caggtgaacg aactgctgga gcgcgagtac ggcatcggcc acaccaccgt
      181 gcagttcgag ttcgcgacct gcgacgtcga cgatccgtac tgcgtgccgt acagcgaggc
      241 gaagtagcgc tacttcactc ttgcgcgcag ctccgactcg tgcgctttcg cacgatcgag
      301 cgccgcttcg agtgcttgca cgactgcgct cgccgaggcc aggagggatt ggagctcggc
      361 tcgttctgct ccggcatcgt gaatcgccgt ggcgctttcg ccgaacggat cgcctgcctg
      421 atcaggctcc ggctcgaccg tggccatcgc accgaacggg tcctcttcct ccgcgacggc
      481 cacaccgtcg tacgactccg gctcgtattc gtgaacctca aatggagaaa ccgccatcgt
      541 cgcctcggca tcgaagggag cagacggtgg agtcggaggc tccgaagatg aggtcgtttt
      601 catcgacagc ttgggagtgc cggcggagct cgaagtcacg cccgacttcg ccgacatgtc
      661 cggcgcggcc agctccggct cctcgtgcaa cgtcgtgtcg acgttcctct tcaggttatc
      721 gagaatccgc gtcactgccg cacgtagcgt ggccgtcacg cccgtgccgt tgatcgcaga
      781 cgcttcgaca gccggtgcgc cgttcgggtt gaggtacgcg ttcatcgctt cgaccgaatc
      841 gacattcggg agatcgcgct tgttgtactg gagaacgaga gcgatcttgt caggatcgat
      901 gcgattgatc cgaaggttgg ctttcagatt ctcgaagctg tcggtattgg agtcgcgcat
      961 cgacgactgc gaatcggcca cgaagaccac gccgtctgcg ccgcgcagaa cgagcttgcg
     1021 cgtggcgtca tagaagacct ggccgggaac cgtgtagagc tggacgcgca ccttctgccc
     1081 cgcgatcgtg cccagctcca tcggcatgaa gtcgaagaag agcgtccggt cggtctctgt
     1141 cgacatggag atcatcttcc ccttgttgtc gagcttcgga ttgccgtaga tcttctccag
     1201 attggtcgtc tttccgcaca gaccgggccc gtagtaaacg agcttgacgg tcacctcatt
     1261 gatcgctttg ttgatcagga ccatattcga agcctcccgg ggcgggtgaa gcttaccgtg
     1321 attggcgggg gtcgggagac gggtgtcggg ggtcggaggg atctggatgg ctactctggc
     1381 tgaaccaact ccgacacccg acccccgaca gccgactccc ctcgaacctg atatcttttt
     1441 cccatgtccc gcaaagagct attcgaaaaa gtcgcgctgg agcagatcat cggagtcgtt
     1501 cgcgaggaga gcgttgaagc ggcggagtcg gtcgcggaag cgtacgcgcg caacggcatt
     1561 cggatcatcg aagtgaccct gaccacgccg caggcgtttg agctcatctc gcgctttgcg
     1621 caaacgtacg ccccgctcga cgtcgtgatc gcggcaggaa cggtgcgcaa ctcgaatgat
     1681 gccgcgcagg cgcgccgcgc cggagcgcag ctcatcgtct cgccacacac cgacgtcacc
     1741 gtgatcgagt acgcaaacga gcacgatctg ctttcgatcg ccggcgcggg cacagctacc
     1801 gagatcattc gcgcgtggga agccggttgc gacatcgtca aagtctttcc cgcgctctac
     1861 ctcggcggtc ccgactacat ccgcacgctc cgcggcccga tccgcgacgt gccgatgctt
     1921 gctggcggtc ccgttccgct cgacacgatc gagaactatc tcgacgccgg ctgcatggcc
     1981 gtgaacctcg gcgcgtcgtt agccgtgccg gatctcgtcc gctccgagca gtgggaggag
     2041 atcggacgcc gcgctcttct ggccacatcg atcgtgcagt cgcggaacgt cgacgtcgaa
     2101 tccgattcct acgtccactg aatcgtgagc ggtctactcc agaaggcgta cctcgtgccc
     2161 gggcagccgc acatcctcct cggcgcggac cgcaacgcag ggtgggcttc gctgaaggcg
     2221 agctacgaag cggtcggccg cgagatcgaa cgctccggcg ccgagttgat cctgctctat
     2281 tcgacgcaat ggttctcggt catcggtcac ctctttcaaa ccgatcccaa tccgacatgg
     2341 acgctcgtcg atcagaactg gtacgacctc ggcgagatcc cgtattcgtt ccgcgtcgat
     2401 ccggaattcg gcgcgctcta cgccggcatc tgcaaggacc tcggcatgca gacagcgacc
     2461 gtggcctatc acggctttcc gatcgatacc ggcacggtcg tcgctctcaa actgctcacg
     2521 ccgaacaacg ccattccggc ttccgtcgtc tcgtgcaaca tctacgcgga gcgcgacgaa
     2581 acccgcgcgc tgggccgcgc cgcgcgaacc gcgatcgagc ggtacggcaa gaagaccatc
     2641 gttgtcatcg tctcgaatct ctccaaccgc ttcgaagtcg gcgagatcga tatggcgcac
     2701 gaccggatct cgtcgtcgaa ggacgatgag tggaatcgca agatcctgga gatgctcggc
     2761 gaagggcggc tcgaggatgt ggcgcaggtg gcgcgcgagt tcggccgcga ggccaacgcg
     2821 gacatggggt tcaaggccat ttggtggctg gctgcgctcg ccggcgagaa caacaaatac
     2881 gacggcaagg tgtgggatta ccagccggtg tggggcaccg gcaacgccat cgtcgagctg
     2941 acgccgaacc cgaacaaaga gctcgatttc gagaaggagt tcgatgaagg accggtcgag
     3001 cagggcacag ggctcgagac gcacaacgtg accgagggcg gtccggatgt tcatcgcgag
     3061 agcgagatga cgtcggaccc gggagtcatc ccaaacgcga gcgagacagc gaccgagccc
     3121 gaattccgcg ttgctcaagt gaagagcagc acggtaatga catcgcgtgc agccgcgcct
     3181 gttggtgcgt atccgcacgc ccgccgtgtc ggcgatcttc tttttctttc cggcatcggg
     3241 ccacggcggc cggtgacgaa tgagattccg acagggatcg aagcgcagac gcgcgccacc
     3301 gtcgacaacg ttcgtgtgat tctcgaagcc gctggcagct cgctggagaa gatcctcgat
     3361 gtgactgtct atctgacaga catgaagcgc gacttcgatt ccttcaaccg cgtttgcgcg
     3421 gagctgctcg ggtcgatcca gcccacacga accaccgtcg gcgtcgactc gctgccgacg
     3481 ccgatcgcgg tggagatgaa gatcatcgcg gccgcacgat gacgctcaga actgatcgcg
     3541 ctgccgcttc gcatcaaagg gggagacgcg agcccgatac gcgcagtctc gagaacgctg
     3601 tgagacgttc atacgcctca atgagccgct cggcagcgcg cgcgtagctg aattcctgcg
     3661 actgctcgta ggcacgctgg cgaaggtccg cgatgtctcc gttcgcaatg ccggcgacct
     3721 tctccgccgc agcctctgga gtgctggcgc tggcgatcag cgcggcactg aggtcgccga
     3781 gcaacgcggg gattccagcg atcggaaaag tgaccggcac gacgccacac gccatggctt
     3841 cgagcaccgc gcgatgacct tcgtccgatc cgcgagcggt aaagaggagg atgtctgcgg
     3901 cccggtaatg ctcggcaaga tcgtcctcgt gatagccggc ccagatcaca tcgatggcca
     3961 gctcacgcgc caatgcttcc agcgcggggc ggtgctcgcc gtgaccgatg atcatcagcc
     4021 gcgcgttcgc gatctcgcgg tgcacgaacg cgtacgtgcg caggacgtcc tcgaaaccgc
     4081 ggtccttcga cagcttgccg atcgccatga ggagcaaggt ctcagggccg aggccgtagt
     4141 gcgaacggac gtcgggtccg tcgggtgtga actgccgctc gtcgagggga ggcggcgtga
     4201 agatggggct ccggcgccgc accggctcca tcgccgcgaa cgagtcgttg ttgacgaaaa
     4261 tcccatccgt gcgggcgatg agccatcgcg tgaacggatc gctgcgaaga acgcgagccg
     4321 catgaaatgt ccggacgatg accggcccac ttccccgcgc ggcaaaaagc gtcagccagt
     4381 gatcgtaggt gagatgcgcg tgaactacgt caaagcgatg gtgttcgatc agccgcgaaa
     4441 tggcgtcggt ggtgcgcagg aacgaccagg gattctgcgc cttgctgatg agcggatggg
     4501 caaagtcgtg atgcccgatc ttttgctgga gcttgtagcc accaacgtac gcgtaatgcg
     4561 cgtcgacccc cgcctgtcgc agcgcctccg cctcggcaaa tgccggagct gctgcgcctg
     4621 tccatcgatt tccggaagcg aggtggagaa ctttcatggt gcgttcggta gtcccgcagg
     4681 cagtcatcca gtttgaagcg cgagtttgca gacacgtgtc agctgattgc ctcgtcgatc
     4741 ggttgggcaa ttcgcattag ttcggcgcgg atgtcctgca tcctgtcttc gtctggcgac
     4801 catacgtttt cgatgacgca gtgaccttct gcgccttcgc gcatatcgcc gtctggattc
     4861 ggatcacgaa aaacatggag atcaaaggca atgccccgaa tcgcttcgac agaaagatga
     4921 aagccccacg gatacccacc cggtttcgtc atcaaagctc gcgcctgggc agctgacgta
     4981 atctctcgat cgtacacgga cagctgtcca catcgtttca gggcggcctc ttttagctcc
     5041 ctactgccca gtgtgaagtc ttgcgcctgc ggagagcgca tgttctcgat gaatagttca
     5101 tcggcacgcc ggcgggcgag cctcagaacc aaaccagcgc tcagttcggg acgagtacca
     5161 gacatctcgc cttagtcgga ctcttcgaac atcgcaataa gatggatctt cagcacatca
     5221 gacggagccg attcgactgt ttgcgcgcgc tggcgatcgc tctgtccaag atcgcgatct
     5281 cgataaaacc agtcatattt ggcggagggc cgaatctcga tttcaaggta gtgtctcccg
     5341 agattccagg acatcgaaag gccgccctcg tctgtagggc tggcgtttgg ccgcgtgatg
     5401 gcaggtaccg aagctcgcaa tccacgccag agatcttccg cggctccggc tacggcaggc
     5461 gtcgccagct tccggctggc cagggagcgc aagtacgaga gccattgctc ttctggcgaa
     5521 agaactgtct gctctatgtt ggcgaatgac atggctgagc cggtaggttg cgtcaatcgt
     5581 acccaaaatg cacgcgaaat acctctgatc cagtgcgtac taccgcgagc gcgacttgca
     5641 ccgccgcgac ctcgcaaccg cgagaccgcg atgttacact ccgcgccgtc atggccatcc
     5701 agccccagct tctcgagatt ctggcgtgcc cgatctgcaa agaagaggtg cggcccgtgc
     5761 cactttcgga agcgaagcgg gtgactgttc gcgacaagtt ccgcgacaaa atgcggggcg
     5821 aggagctcgt cgttgaagag gggctgcagt gcgtgaagtg ccaccgcgtc tacccgatcg
     5881 tcagcgacat accggtcatg ctcgtcgacg aagccttcga agaggcgtga cgccacgccg
     5941 tgttcctcaa gcgtcggctg gacgttcctg aagaaacacc actccccgtc gcgcttgctc
     6001 tctttttcta cgtcggtcac cttttctgcg ccggctgggt agcgtcgtcg gaagtcttcg
     6061 cctttgccac gaccgtgatg gtcggctggt gtctctggcg gcgtctcctt gagccgtcgt
     6121 ttcacatcct ctatgtaccc ctcgcgctgt acgggctggc gtctacggtt tctgcgctgg
     6181 ttgcggtgcg cgaaatccac tcgttcgggg agacggcgtt gtggggaaag atgctgctct
     6241 ttccggcggc gctgatcctg ctccgcaacg tcccgcaagc gcgattcacg gcactgcgca
     6301 tggttctcat cttcggggcg atggcgtcag ccatgggtct ggcccaatac gtcactgctg
     6361 gcggctttcg cgatctcgaa catcgcatca ccggcccgac cgcacacgtg atgacgttgt
     6421 ccgggttact gctgccggtg gcgctgacgt tcatcgtgct ctggatttac gaagccggga
     6481 atctctggct gctcgccggc gcggccgtga cgaaccttgc ccttctcatc acctttacgc
     6541 gcagtgcgtg gcttggatgg atcgtcggca tcgccgtgct gctgatcctg aagtggccca
     6601 aggcgctggc gtgggccgtt ccggtggcga tcatcgccat caattttgcg ccgctgccgc
     6661 tgttcagccg ggtgatctcg agcttcgaca cgcgacagtc gtcgaatctg gatcggatcc
     6721 ggatggccga ggcaggtgtc gagatcatca aagactaccc ggtcttcggc gtgggaccgg
     6781 cgaacgtcaa agagatctac cccctctacc ggaagcacga tgcgccgcgt ttccgtatcc
     6841 cgcacctgca caacaacgtg atccagcttc tggcggaacg cggcgttctg gcccttctgg
     6901 cgtatctctt tctgcaggga ctcttccttc gagaatgtgc gcgcgcctgg ggcggtccgg
     6961 caacgaaata cgccgagatc ggcgtagtgg tcgtcgccag tctcggggca gcggggatgt
     7021 tcgagttcaa cttcggcgac accgaggtct tctggatcat gctcgacctc ttcgcgatcg
     7081 tta
//
LOCUS       DATLVW010000670        10756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_154682, whole genome shotgun sequence.
ACCESSION   DATLVW010000670 DATLVW010000000
VERSION     DATLVW010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154682"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..585)
                     /locus_tag="VNN25_19460"
     CDS             complement(160..585)
                     /locus_tag="VNN25_19460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HXH93767.1"
                     /translation="MDVHDKATRSFNMSRIRSRDTKPELRLRKLLWARGLRGYRVHSK
                     LPGKPDLAWSRGKVAIFVDGCFWHGCTKCGDGRAPSSNTSYWDAKRLSNKQRDARRTK
                     ELEQMGWRVLRLWEHEVLKESLRSAKRVEKLLGQARRGR"
     gene            652..1656
                     /gene="dcm"
                     /locus_tag="VNN25_19465"
     CDS             652..1656
                     /gene="dcm"
                     /locus_tag="VNN25_19465"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HXH93768.1"
                     /translation="MDLKVVEICAGGGGQALGLEHAGFEHLAALEIDHHACATLRLNR
                     PEWKVIEEDLHHFDGTPYRGRTDLLAGGVPCPPFSIAGKQLGSDDDRDLFPEAIRLAA
                     EIQPRAVMLENVPGFASEKFKDYRTALMARFRRLGYQVEWRILNACDYGVPQLRPRFI
                     LVALRKEDMQFFRWPEPEERKRLVGEVLVDLAGANGWKGATAWRARANGIAPTVVGGS
                     KKHGGPDLGPTRARQQWAALGVDGLGIADEPPSQDFPREASLRLTVRMVARVQGFPDA
                     WQFAGRKTASYRQVGNAFPPPVAEAVGRSIVAAFEGIAEKSFDREQQRLFDRAEAAIL
                     "
     gene            1660..2241
                     /locus_tag="VNN25_19470"
     CDS             1660..2241
                     /locus_tag="VNN25_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXH93769.1"
                     /translation="MPKKPRAPHQPGSKAKILYFLLANIGRVVTSDEIREASGNKSEW
                     ARRLRELRNEEGYQILSHNDRADLKPGEYLLETDKRLPAFARKISKETRAAVLARNGN
                     TCQLCGVSAGDTDPYDPSRTIRLTMGHIIDSSKGGTDQASNLRALCSNCNEGLQNISP
                     PKPSLIDLLTFIRRATRDDQRAALEWLRKKFKD"
     gene            complement(2320..3954)
                     /locus_tag="VNN25_19475"
     CDS             complement(2320..3954)
                     /locus_tag="VNN25_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93770.1"
                     /translation="MEVVPRLTKFFTQLISETSGLSLADTLALAEHAPQLDADMVLAH
                     EIREFRELLGRFRVDAAPIESLLTHAVARSLADFFRAFPIPFRDEHIHLTGSLDADFI
                     WPRLAPLLDGPERAMYEAKIAEVYGPGALPIASGADVDRLIRLGDDDRFDRYLKILYL
                     AKLVLTSREAHRDAAYHMAKRLFRDANVGSIRLKFTLFRETTDSSEQIPGISDLTAED
                     VMLGLYEGFRAFQSEVPAFRFILSPSFRKEPGFYDATRYPTKRASFDDQVRQIIELLK
                     KHPELRDVVTEVDTVGNERGFYRKAHFQEMRVGFRKLQAYGFRTRSHNGETWNTLRQG
                     IQAVDNAMNIWYVDAIEHGLSLGINPNFYFHRMLQRVLTANGRSEPVAHGTREHDELL
                     DMDWSGRDVIREKLLRGEPLTDYEATLATKTKFHHAREVEHYQHDVLNRMIDKRLTLV
                     SLPSSNKKLTGALSDYKDHPFSWWEKKGLRLGVGTDNDVTLNTNFLREMLILLLTDPL
                     ELKITKLLIVTTGESRRSHLSHLLWQMRKAAEGGQR"
     gene            complement(3991..4332)
                     /locus_tag="VNN25_19480"
     CDS             complement(3991..4332)
                     /locus_tag="VNN25_19480"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HXH93771.1"
                     /translation="MTTFQISAFVFLGLALAATISFRLRRQASFRVAFAWALLWIGAA
                     IAIAWPQITVRVAHFLGIARGADLVFYLAILGMFVGFFAVYVRFRRMESEMTRIVREL
                     ALRTPKEPDSE"
     gene            complement(4329..5018)
                     /locus_tag="VNN25_19485"
     CDS             complement(4329..5018)
                     /locus_tag="VNN25_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH93772.1"
                     /translation="MTGVVKDTTFIVIPAFNEERAIGVVVAELKVLWPHLIVVDDGSH
                     DATASVAQQSGATVLRHAVNRGQGAALQTGITHALRRGAQIVVTFDSDGQHRAEDVAT
                     LVAPIEEGRADVVLGSRFLGSTENMPALRGLLLHAATAFTRMTSGARVTDAHNGLRAF
                     SRAAASRLEIRLDRMAHASEIVDQVAEHGARCVEVPVHVRYTDYSKRKGQTGLGAFRV
                     LLDYLWGRWLR"
     gene            complement(5015..6925)
                     /locus_tag="VNN25_19490"
     CDS             complement(5015..6925)
                     /locus_tag="VNN25_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93773.1"
                     /translation="MVILLTALALLVIGTEITLRAWSDAGPLERATTASVIAAALWLG
                     STWALALLHQLTMTALVARAVIAAIVAVVVIRHRLRGINQHVTIDRRTLVIVAIALLP
                     LLAWTDFMFWRGAIIPPVSHDALSYHLPRAVLFARSHGFRYLTELEARERNIPANYEI
                     LLTEFVVTQHNDTYTEWPSTAFYVLFVIACGALAERWWGRRLLPLLVMMMFVAGIPVL
                     LLHSGAHKNDLMVAFFTVAAMIFAGRWWRDAHRGALLLLIASIAMAVGTKPQAAGFAV
                     FIAPFVLYRMVKCLRPRVIAGVIAFSLAAFLLLGGAVYVVNIVEQHALFGKDTTKSDI
                     ITYGDWKNLWEGPYVLLAAPFSRDGRALAVPWEAHSWFWRRYEIYFSHLGIPFALCAI
                     AAPLAALWFLRSASHSECLVITFAAIAAFILMLPVLFRPHGFYLISLPRYALFIVPIV
                     FGWTVVPLAGRIPERAAEALLVIAALSFVAYGVSNAVNDRFAPLDFVLWAREHPGTRT
                     IPFDQDRAASVADRLAGPNDTIAIDASYASWIHPVFGAGLTRPVIFIPPGNGPPVIPK
                     EAQWVVIDRSWSIIWEASGLRDLSQAEEMMSHGTPSEDDLRVARTVVADPHFKLEYAQ
                     PVRNQLVFRRIQ"
     gene            complement(6958..8751)
                     /locus_tag="VNN25_19495"
     CDS             complement(6958..8751)
                     /locus_tag="VNN25_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93774.1"
                     /translation="MRQIRLSHVLFAGAFLWIGLLLQRGRPLHWDEIEFFRATRWIGE
                     GRIPYAGFWEHHSPLQWFLFAPFARFLATAPGVSAIVVMRWVQVPLWIGVFALIVVIS
                     RRVAAAAWGHVAMVAMLLASPLFVFSALEYRVDTLGNACFLAAVALLVTRDVTRGAAI
                     AFGVLGSCAVLANIRMAPLIVIAALFALVWMRSEERWRWNGRALWMMPGVAFVAAAFL
                     GWLRLAGAWDGFLDGVVRYNSVSNSLVHIAANTFVDRLLDPVITPDLAMIVAWIAALA
                     GVAIALRSIRRPSPPVIIALLFIGTVVTLVPLGVHYPYHLQTTYLLMPALGAIAADRL
                     LQDGKRAEMIRGVAAGIVALALLIQYASLMRSPFGAAMDYQDRVMRTVDERTTKSDTV
                     WDGVGYALHRQPAYRYWFLPAGVRLMAENALIQQYDIARDPPAMIVHGYRVNLWMRSF
                     PRVTSYAVHHYVPLYRDLWIPGMSATIEPRPMRTMWRVPASGRYTIYASEILLRHPWF
                     TRPLEYAMSEAEAPETYEIPLSLLPLLPASDDLQWSIDGRSVPAGARTLDLAKGSSLV
                     LTSSLRNRAGILIVPADVRTLCVGPEVPVAF"
     gene            8851..9678
                     /locus_tag="VNN25_19500"
     CDS             8851..9678
                     /locus_tag="VNN25_19500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH93775.1"
                     /translation="MPSGDAPPLTVFTPFYKNIEYFDETIVSVLSQSFRDFEYLMIND
                     GDPAVAAAIQARYDDPRIRIINTPSPLGLGGSRNAGLHEARGALIAFIDSDDVCEPGR
                     FEKQEAFLRSHPDHVLVGSALRYINAQSRTIGRRRYPENDEDIKKRMVVMNCIAQPSV
                     MARRTALIEAGGYVPEFPAAEDYDLWLRAARLGKFHNLPEELVAYRIHPAAGKNLLLK
                     PALRDSTRLKIRAIRRYGFHATPRALASIVMHSLLLLLPSRAIFWLFNRLIVNRQRE"
     gene            complement(9644..>10756)
                     /locus_tag="VNN25_19505"
     CDS             complement(9644..>10756)
                     /locus_tag="VNN25_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93776.1"
                     /translation="SSALSFIFVSTMALSTIAPAVSRVVARLVARGDVGRVDALRSFV
                     RSRFRRWSAAAIVVSCAAAIPLARLFHFRSVWTPMLAFLCAIAFALASIERGFLQGLG
                     RFRTQNLNTVFEAGLRLVLALVLLAIAQRPDSALVAYLISMSIATAMLLRHGESEAGG
                     GDDWREVVRLASPLLLLLLGSAVYQNADMLAVKRWLPAAIAGQYGAASAMTRAFGVLF
                     VPLYIISGPMLVALHEEGRSIISATLRLCAYFLALCAVPLALLTWYGREITTLLYGAV
                     YAEAGSIAAALGGVLVIGYLTLLIAQARVTVGAFGVARIYAVFGVLQIALLMVMHDSV
                     GSIITALIVVQGALLLVVLAALFRPITRADDSRSDD"
BASE COUNT         2270 a         3355 c         3345 g         1786 t
ORIGIN      
        1 cctcatcgtc gagcgcccgc acctccgcca acacgcgctc ctgcgcctct tcaaccgcaa
       61 tcatccgctc gccggcgcgt ggtgtctgga aggtggtcat ggtttcgatt catctcgtcg
      121 cgcacaaact accgggcgtg gagcactctt tgatgcggct tatcgtcctc ggcgcgcctg
      181 acccagaagc ttctcgacgc gttttgcaga tcttagcgat tctttcagga cctcgtgctc
      241 ccaaagccgg aggactctcc accccatctg ctcgagctcc ttcgtccggc gcgcgtcgcg
      301 ttgtttgttg ctgaggcgct ttgcatccca gtagctcgtg ttcgaggacg gagcgcgccc
      361 atcaccgcat ttcgtgcatc cgtgccagaa acagccgtcg acaaagatcg ccactttccc
      421 tctcgaccaa gcgagatcag gcttgccagg gagttttgag tgaacgcgat aaccccgtaa
      481 gccgcgcgcc cacagaagct tccgcagccg cagctcgggc ttagtgtcgc gcgagcgaat
      541 cctcgacatg ttgaacgaac gagtggcttt gtcgtggacg tccatgagag tcttgttcta
      601 cattcggtag aagttctttt ttcctcggac ccttcccgct acactgcctt cgtggacctc
      661 aaagtagtcg agatatgcgc cggcggcggc ggccaagcgt tgggtctcga gcatgccggt
      721 tttgagcacc ttgccgcgct ggagatcgat catcacgcgt gcgcaaccct tcgactcaac
      781 cggcccgagt ggaaggttat cgaagaggat ctccatcact tcgatggaac gccatacagg
      841 ggccggacgg atctgctggc aggcggagtc ccatgcccgc ccttctcgat cgccggaaag
      901 caactcggca gcgacgatga tcgcgatctg tttcccgaag ccatccgcct cgctgctgag
      961 attcagccgc gagcggtgat gctagaaaat gttcccggat ttgcttcgga aaaattcaag
     1021 gactaccgca ccgcgttgat ggcaagattc cgccggctcg gataccaggt cgagtggcgc
     1081 atcctcaacg cttgcgacta cggcgtccca cagctccggc cacggttcat cctggtcgcc
     1141 cttcgcaaag aagacatgca gttcttccgc tggcccgagc cggaggaacg caagcgattg
     1201 gtaggcgagg ttctagtcga tctggctgga gcgaacggct ggaagggagc aacagcctgg
     1261 cgtgctcgcg caaatggcat cgctcccaca gtcgttggag gttccaagaa acacggcgga
     1321 cccgatctcg gcccaacgcg cgcccgtcag cagtgggctg cactcggagt cgatggtctc
     1381 ggcatcgctg acgaaccgcc ttcgcaagat ttcccaagag aggcgagcct cagactaacc
     1441 gttcggatgg ttgcgcgcgt acaaggcttc cctgacgcgt ggcagttcgc cggccgcaag
     1501 accgccagtt accggcaagt gggaaacgcc tttccgccac cggtcgctga agcagtcggg
     1561 cggtcaattg tcgcggcttt cgaaggaatc gccgagaaat ccttcgaccg cgagcagcaa
     1621 cgcctctttg atcgtgcgga agccgctatc ctgtagcgca tgccgaaaaa gccacgcgct
     1681 ccgcatcagc ccggatcaaa ggcaaagatc ctctatttct tgctcgccaa catagggcgt
     1741 gttgttacat cagacgagat ccgcgaagca tctggcaata agtcggaatg ggctcgccgg
     1801 ctccgagagc ttcggaacga agagggctat cagatcctct ctcacaacga ccgcgccgat
     1861 ctaaaacccg gcgaatacct cctcgaaacg gacaagcggt tgccagcttt cgcacgcaaa
     1921 atctcgaagg aaacaagggc ggcggtcttg gcacgcaacg gaaacacgtg tcagctttgc
     1981 ggcgtatcgg ctggggatac cgatccgtac gacccaagcc ggacgattcg tctcaccatg
     2041 gggcacatca tcgacagttc gaagggcggc acagatcagg cttcgaacct ccgcgcgctt
     2101 tgcagcaatt gtaacgaggg cctgcagaac atctcgccac caaagccttc gctgattgac
     2161 ctgctcacat tcatcagaag agcgacacgg gacgatcaac gtgccgctct cgaatggctt
     2221 cgtaagaaat tcaaagacta acgctgagac ggcggccctt tgcgtaccac ccaaactctg
     2281 aaatcgagag acgtgccgaa caagaacacg gacgtcgccc tacctctgcc ctccctcggc
     2341 cgccttccgc atctgccaaa gcagatggct gagatgcgat cttcgcgatt ctcccgtcgt
     2401 cacgatcagg agctttgtga tcttcagttc cagcggatcc gtcaacagca ggatcagcat
     2461 ctcgcggagg aagttcgtgt tcagggtgac gtcgttgtcg gtaccgacgc cgaggcggag
     2521 gcctttcttt tcccaccagg agaaggggtg gtctttgtag tcgctgaggg cgcccgtcag
     2581 tttcttgttc gatgacggga gcgacaccag cgtcaggcgt ttgtcgatca tgcggttgag
     2641 cacgtcgtgc tggtagtgct cgacttcgcg ggcgtggtgg aacttcgttt tcgtcgccag
     2701 cgtggcttcg tagtcggtga gcggctcgcc gcgcagaagc ttttcgcgga tgacgtcgcg
     2761 gccgctccag tccatgtcga gcaactcgtc gtgctcgcgg gtcccgtgcg cgaccggctc
     2821 gctgcggccg ttcgcggtga ggacgcgttg gagcatgcgg tggaagtaga agttgggatt
     2881 gatgccgagg gagaggccgt gctcgatggc gtcgacgtac cagatgttca tggcgttatc
     2941 gacggcctgg ataccctgcc gcagcgtgtt ccacgtctcg ccgttatggc tgcgcgtgcg
     3001 gaagccgtac gcctgcagct tgcggaagcc gacgcgcatc tcctgaaagt gcgccttccg
     3061 ataaaacccg cgctcattgc ccaccgtgtc gacctcggtg acgacatcgc gcagctcggg
     3121 atgtttcttc agcagctcga tgatctgccg cacctgatcg tcgaagctgg cgcgcttcgt
     3181 cggatagcgc gtggcatcgt agaagccggg ctctttgcgg aagctcggcg agaggatgaa
     3241 tcgaaaggcc ggaacctcgc tttgaaacgc gcggaaccct tcgtacaacc cgagcatcac
     3301 atcctcggct gtcaaatcgc tgatgccggg aatctgttcc gacgaatcgg tagtctcacg
     3361 gaagagcgtg aacttcagtc gaatggaccc gacgttggcg tcacgaaaga ggcgcttggc
     3421 catgtggtat gcggcgtcgc gatgcgcttc gcgcgacgtg agaacgagct tggcgaggta
     3481 gagaatcttg agatagcgat cgaagcggtc gtcgtcgccg aggcggatga ggcgatcgac
     3541 gtccgcgcca ctggcgatgg gaagcgcacc ggggccataa acctcggcga tcttcgcttc
     3601 atacatcgcc cgctccgggc catcgagaag aggcgcgagg cgcggccaga tgaaatcggc
     3661 gtcgagcgag ccggtgagat gaatgtgctc gtcgcgaaac ggaatcggaa aggcgcgaaa
     3721 gaaatcggcg agcgaccgcg ccacggcgtg agtgaggagc gattcgatcg gcgcggcgtc
     3781 gacgcggaaa cggccgagga gctcgcggaa ttcgcggatc tcgtgcgcaa gaaccatgtc
     3841 cgcgtcgagt tgcggcgcgt gctcggcaag cgccagcgtg tcagcgagcg acaagccgct
     3901 cgtctcgctg atgagctgcg taaagaattt cgtcagccgc ggaacgactt ccatgccgcg
     3961 catgctatcg cgggcggcaa ccgcaagcac tcactcggag tcgggctctt tgggagtgcg
     4021 caacgcgagc tcgcgcacga tccgcgtcat ctcgctctcc attcttcgaa agcggacgta
     4081 gaccgcgaag aagccgacga acatgccgag aatcgccagg tagaaaacga ggtccgcgcc
     4141 gcgcgcgata ccgaggaaat gcgcgacacg caccgtaatc tgcggccatg caatcgcgat
     4201 cgcggcaccg atccacaaca gcgcccatgc gaaggcaacc cggaaggacg cttgccgtcg
     4261 cagccgaaac gagatcgtcg cggccagagc aaggccgagg aagacgaagg cggaaatctg
     4321 aaacgtagtc atcgcagcca gcgcccccaa agataatcca acaatacgcg gaaggcaccg
     4381 aggccagtct ggcccttgcg tttcgagtag tcggtgtaac gcacgtgcac cggcacctcc
     4441 acgcaacgag cgccatgctc ggccacctga tcgacgatct cgctggcgtg ggccatccgg
     4501 tcgagacgga tctcgagacg cgacgccgcc gcgcgcgaga acgcacgcag cccgttgtgc
     4561 gcgtctgtca cgcgcgcgcc tgaagtcatg cgcgtgaacg cggtcgcagc gtgaagcagg
     4621 aggccgcgca acgcgggcat gttctccgtt gagccgagga atcgggagcc gaggacgacg
     4681 tcggcgcgcc cttcctcgat cggcgcgacg agtgtcgcga catcctcggc gcgatgctgg
     4741 ccgtcgctat cgaaggtcac gacaatctgt gcaccacgcc taagcgcgtg cgtgatgccg
     4801 gtctgcaatg ccgcaccctg gccgcgattg acggcatgcc gcaacaccgt cgctccgctc
     4861 tgctgcgcca cagaagccgt ggcatcgtgc gatccatcgt cgacgacaat caaatgcggc
     4921 cagagcacct tcagctccgc cacgaccacg ccgatggcgc gctcttcgtt gaacgccgga
     4981 atgacgatga acgtcgtgtc cttcacgacg cccgtcattg tatccgtcgg aacacgagct
     5041 ggtttcgcac gggctgcgcg tactcgagct tgaaatgcgg atcggcaacg acagtacgcg
     5101 ccacccgcag atcgtcctcg cttggcgtgc cgtggctcat catctcctcg gcctgactga
     5161 gatcgcgaag gcctgacgct tcccagatga tcgaccagct tcgatcgatc acgacccact
     5221 gcgcctcctt cgggatgacg ggaggcccgt tgcctggcgg gatgaagatg accggtcgcg
     5281 tgagtccggc tccgaacacg ggatggatcc acgatgcgta cgaggcgtcg atggcgatcg
     5341 tatcgttcgg gccggcgagc cggtcggcga cgctggcggc gcggtcctgg tcgaaaggaa
     5401 tcgtgcgcgt gccgggatgc tcgcgcgccc agagcacgaa gtcgagcggc gcgaagcggt
     5461 cattgaccgc gttgctgacg ccgtacgcga cgaatgacag cgccgcgatg acgagtagcg
     5521 cctcggcggc gcgctccggt atccgcccgg cgagcggaac aactgtccat ccgaagacga
     5581 tcggaacgat gaagagcgca tagcgcggca gcgagatcag gtaaaagccg tgaggccgga
     5641 aaagaacggg cagcatcaag atgaacgccg caatcgcggc gaacgtgatc acgagacact
     5701 ccgaatggct ggcgcttcga aggaaccaca acgccgcgag gggcgcggcg atcgcgcaaa
     5761 gcgcaaacgg gatcccgagg tgcgaaaagt aaatctcgta gcggcgccag aaccacgaat
     5821 gcgcctccca cggcaccgcc aacgcacgcc cgtcgcgcga gaacggcgct gccagcagca
     5881 cgtatggccc ctcccagagg ttcttccaat cgccgtacgt gatgatgtcg ctcttcgttg
     5941 tgtctttgcc gaatagcgcg tgctgctcga cgatgttgac cacgtaaacc gcgcccccca
     6001 acagcagaaa cgcggcgagc gagaatgcga tcacgccggc gatgacgcgc ggcctcaaac
     6061 acttcaccat tcgatagaga acgaaaggcg cgatgaagac cgcaaatccg gccgcctgcg
     6121 gtttcgtccc gactgccatc gctatcgatg cgatgagaag cagcaacgcc ccgcgatggg
     6181 catcgcgcca ccaccggccc gcgaagatca tcgcggcaac tgtgaaaaac gccaccatca
     6241 gatcgttctt gtgcgcgccg ctgtgcagga gaaggacggg gatgccggcg acgaacatca
     6301 tcatcacgag cagcggcagg agacgccgtc cccaccatcg ctccgcaagc gcgccgcacg
     6361 cgatcacgaa gagcacataa aacgccgtgc tcggccactc cgtgtacgtg tcgttgtgct
     6421 gcgtgacgac gaattcggtt agcaggatct cgtaattcgc ggggatgttt cgctcacgcg
     6481 cttcgagctc ggtcaggtaa cgaaagccat gcgaccgagc gaagagaacg gcgcgcggaa
     6541 ggtgatacga gagtgcgtca tgactgaccg gagggatgat cgcgccgcgc cagaacatga
     6601 agtcggtcca ggcaagaagc ggcaggaggg cgatggcgac gatgacgagt gtgcggcggt
     6661 cgatcgtcac gtgctggttg atgccgcgaa ggcggtgacg aatcaccacg accgcaacga
     6721 ttgcggcgat gacagcgcgt gctacgagcg ccgtcatcgt gagttggtgc agcagggcaa
     6781 gagcccatgt cgagccgagc cacaacgccg ccgcgatcac cgatgcggtc gtggcgcgct
     6841 cgagcggccc ggcatcgctc caggcgcgga gcgtgatctc cgttccgatc accagcagcg
     6901 caagcgccgt gaggagaatc accatgagag cgcgcgcaga atagcaggac gcgatcgtca
     6961 gaacgcgacc ggcacttcgg ggccgacaca aagcgtgcgg acatcggcag gcacgatcag
     7021 gatcccggcg cggttgcgta gcgaggaggt gagcacgagc gatgatcctt tcgcgaggtc
     7081 gagggttcga gctccggcgg gtacggaccg gccatcgatc gaccattgga gatcgtcgct
     7141 cgcaggaagg agcggcagta gagagagcgg gatctcgtag gtttctggtg cctcggcctc
     7201 gctcatcgcg tactccagcg ggcgagtgaa ccagggatgg cgcaggagga tctcgctcgc
     7261 atagatggtg tagcgcccgc tcgccggaac ccgccacatc gttctcatcg gccgcggctc
     7321 gatcgtcgcg ctcattcccg gtatccacaa gtcgcgatac aagggaacat agtgatgaac
     7381 cgcgtaggac gtaacgcgcg gaaatgaacg catccagagg ttgacgcgat acccgtgcac
     7441 aatcatggcc ggaggatcgc gcgcgatgtc gtactgctga atgagcgcgt tctcagccat
     7501 gagccggacg ccggcgggaa ggaaccagta gcgatacgca ggctggcgat gaagcgcgta
     7561 accgactcca tcccagaccg tgtcgctctt cgtcgtccgt tcgtctaccg tgcgcatgac
     7621 gcgatcctgg tagtccatcg ccgcgccgaa aggtgatcgc atcaggctcg cgtattggat
     7681 cagcagtgcg agggccacga ttcctgctgc gacaccgcga atcatttccg ctcgcttgcc
     7741 gtcctgcagg aggcgatcgg ccgcaatcgc accgagcgcc ggcatcagca gatacgtggt
     7801 ctgcaggtga tagggatagt gaacgccgag tggcacgagc gtgacgacgg tgccgatgaa
     7861 gagcagcgca atgatgacgg gtggcgaagg cctccgaatg cttcgcagcg cgatcgcaac
     7921 gccggcgagc gctgcgatcc aggccacgat catcgccaga tcaggtgtga tgaccggatc
     7981 aaggagccgg tcgacaaagg tgttcgcggc aatgtgaacg agcgagttcg aaaccgagtt
     8041 gtagcgcact acgccgtcga gaaagccatc ccacgcccct gccaggcgca accatcccaa
     8101 aaacgcagcg gcaacgaaag caacgcctgg catcatccac aacgcgcgtc cgttccatcg
     8161 ccaacgctcc tcggaacgca tccacacaag cgcgaaaagc gcggcgatca cgatcaaggg
     8221 tgccatgcga atgttcgcca gcaccgcgca tgaaccgagg actccgaacg caatggcggc
     8281 tcctcgggtc acgtcgcgcg tcaccagcaa agccaccgcg gcgaggaagc aggcgttgcc
     8341 gagggtgtcg acgcgatact cgagcgcaga gaaaacgaag agtgggctgg caaggagcat
     8401 cgcgaccatc gccacgtgtc cccaagccgc agcggcaacc cgccggctaa tgacgacgat
     8461 gagtgcaaag acgccgatcc acagaggcac ctgcacccaa cgcatcacga cgatcgcact
     8521 cacacccggt gcggtcgcca ggaacctcgc gaacggtgcg aacagaaacc actgtaacgg
     8581 cgagtgatgc tcccagaaac cggcgtacgg gatccgtccc tctccgatcc aacgtgtggc
     8641 gcggaagaac tcgatctcat cccagtgcaa cggccgcccg cgctgcagga gcaatccgat
     8701 ccagagaaat gcgccggcga aaagtacgtg cgaaaggcgg atctggcgca tcactgcgat
     8761 ttcattgtcg cacgcggtgt cgagggaggg ccgccgcgca cgtgagcgtg ttgtaggatg
     8821 caggcacgcg attcggcaac gattccatcc atgccttccg gtgacgcacc gccgctgacg
     8881 gtcttcacgc cgttctacaa aaacatcgag tacttcgatg aaaccatcgt cagcgtcctg
     8941 tcccagtcct ttcgcgactt cgagtatctg atgatcaacg acggcgatcc tgccgtcgcg
     9001 gcagcgattc aggcgcgcta cgacgacccg cgcatccgga tcatcaacac accgtcgccc
     9061 ctcggactgg gtggttcgcg caatgccggg ctgcacgaag cgcgcggagc gctgatcgca
     9121 ttcatcgact ccgacgacgt ctgcgagccc ggccgcttcg agaagcagga ggcgttcctg
     9181 cggtctcacc cggatcacgt gctcgttgga tccgcgttgc gctacatcaa cgcgcagtcg
     9241 cggacgatcg gccgtcgccg ctatccggag aacgatgaag acatcaagaa gcgcatggtc
     9301 gtcatgaact gcatcgccca gccgtccgtg atggctcgca ggaccgcgtt gatcgaagcg
     9361 ggaggctatg tgcctgagtt ccccgcggcg gaggattacg acctctggct gcgcgctgcc
     9421 agactgggaa agtttcacaa cctaccggaa gagctcgttg cctatcgcat tcacccggca
     9481 gccggaaaga acctgctgct gaaacctgcg ctgcgcgact ccacgcgcct caagatccgt
     9541 gcgattcgcc gctacggatt tcacgcgaca ccgcgcgcac tcgccagcat cgtcatgcac
     9601 tcgttgctgc tgttgctgcc gtcgcgggca attttctggt tgttcaatcg tctgatcgtg
     9661 aatcgtcagc gcgagtgatc ggtcggaaca gtgcggccag gacgacaaga agcaacgcgc
     9721 cctgcacgac aatcagtgcg gtgatgatcg agcccacgct gtcgtgcatg accataagca
     9781 gagcgatttg cagcacgccg aaaaccgcgt aaatcctggc caccccgaag gcaccgacgg
     9841 tgacgcgggc ctgggcgatg agcagcgtca gatagccgat cacgaggacg ccaccgagcg
     9901 ccgcggcgat cgatcccgcc tcggcataca cagcaccgta gaggagcgtc gttatctcgc
     9961 gcccgtacca cgtcaacagc gcgaggggga cggcacaaag cgcgagaaag taagcgcaga
    10021 gccgaagcgt cgcgctgatg atcgatcgtc cctcctcgtg cagcgcaacg agcatcggtc
    10081 ccgagatgat gtagagcggg acgaacagca cgccgaacgc gcgcgtcatc gccgaggcgg
    10141 cgccgtactg gcccgcgatg gccgccggca gccagcgctt cacggcgagc atgtcggcat
    10201 tctgatagac ggcgctacca agaagcagaa ggagcagcgg cgaggctaag cgcaccactt
    10261 cgcgccaatc gtcgccgcct cctgcttcac tctcaccatg acgcagcagc attgccgtcg
    10321 cgatgctcat agagatcaga taagcgacca gtgccgagtc cggccgctgc gcgatcgcca
    10381 ggagaacgag tgcaagcaca aggcgcaagc cggcttcgaa gaccgtattc aggttctgcg
    10441 tgcgaaaacg acccaatccc tgcaggaagc cgcgctcgat actggcgagc gcgaacgcaa
    10501 tcgcgcataa gaacgcgagc atcggcgtcc agacggagcg gaagtgaaag agccgcgcga
    10561 gaggaatcgc ggcagcgcac gaaaccacga tcgcggctgc cgaccagcga cgaaaacggc
    10621 ttcgcacaaa ggaccgaaga gcgtcgacgc gcccaacatc gccacgcgca accagtcgcg
    10681 cgacgacacg cgacaccgcc ggcgcaatcg tgctcaacgc catcgtcgag acaaagatga
    10741 acgacaacgc cgacga
//
LOCUS       DATLVW010000671         8533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_26858, whole genome shotgun sequence.
ACCESSION   DATLVW010000671 DATLVW010000000
VERSION     DATLVW010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26858"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /gene="uvrA"
                     /locus_tag="VNN25_19510"
     CDS             complement(<1..1714)
                     /gene="uvrA"
                     /locus_tag="VNN25_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXH93777.1"
                     /translation="MESIVIRGAREHNLKNIDVVVPRNKFVVITGVSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLEQMEKPDVDSIEGLSPAISIEQRTTSRNPRSTVGTVT
                     EIYDYLRLLYASVGVPHCTNCGKEIRPQTIQQMVDRLMTLKTGTKFTILAPYIRGKKG
                     EYRKQMLQMVKEGFTRAIVNGTMIELADPPTLDKQKKHTIDIVIDRLVVKEGIEQRLA
                     DSLETATRVTKGLVKILYQEGNREELLSQSYACPDCGVSIGEITPRLFSFNSPYGACP
                     RCSGLGVLLEVDEKKIIPDTLKSIEDGAISIWKEGAENWRLRQIHTLAKHYKFKLDVP
                     WKSLPEKARSVILQGSEEKIKFQFSGAESTYSYSGTYEGLVPMLTRRYKESDNDDIRE
                     EIERFMTPMDCPDCKGRRLKPEALAVTVAGQPIDEVVGLQLKDAENFFATIDLTPREE
                     KIAGKILKEVRDRLGFLCAVGVGYLMLDRSAATLSGGEGQRIRLATQIGSKLMGVLYV
                     LDEPSIGLHQRDNRKLIDSLLELRDLGNTVIVVEHDEETIETADHIIDLGPRAGVHGG
                     EVVAE"
     gene            1899..4331
                     /locus_tag="VNN25_19515"
     CDS             1899..4331
                     /locus_tag="VNN25_19515"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH93778.1"
                     /translation="MAAILLYLATTVGVIVLWRRFVQPISRMAALVLILLPMCFTGRA
                     LLTGRVYAPIDLPFMSEPLNAYAAEFNVPKVHNGTLSDLYCQIIPWRSAVRTAIFSGQ
                     WPLLNPFMLCGDILAAAAQPAPYDPFNLLGMVIPMPPSLTFAAAMTFFLAGFGAFAFA
                     RALGCGELASLVAAAGWMFSANIAFFVGWPLSRSWAYLPLILFAVQRLVNEASVRAGV
                     LLMIALTSLILAGHPESLLHVTAVGAAYGLFEIATTRKRVGRVIALAAGAGVVALLLT
                     AIFLLPFLEASQQTLELHNRLGYRSMSFPFMPEMIQRRAANTVFPFWGGQPWRDSFTA
                     EWDPQTARTGSIVLALALTALIVARKRKETWFFFALALICLCAGFDAPPMANVLHKIP
                     LFDVALNERLAYAASCALAILAALAVDAFATEVGGSTRRRVAAVVAGGIAIVLGVGTW
                     LVWAHELKVGVDPSVIRINALGDLIPLVIVAILLASRTPLRIAIPLVLVLLLGARIIV
                     DGSMYPSLPQHDFYPKIPIIRAIPENTPEPFRVAGEHFVLIPDSAAIYQLEDPRGYEA
                     MTFARLADTYPVWSIPQPVSFNAIGDLQPPFLSFLNIKYAIVPISVDLTPGWRELMKD
                     RSAKLFENTHVLPRAFVPRRIRYERDGVLALKAMRTAKDFSDMAWIEVPEDKPHEIAN
                     GPGTLTLHRDGFGLDIDATMAADGWVVASQSAWKGWRAYIDGRRVQMRFANHAFLGVF
                     VPAGRHHVKLQYLPESFTRGRAISFATLGLLIVLAGGTTLRRKRSETRAEIRDKRLET
                     RSETDASSGVIV"
     gene            complement(4626..4958)
                     /gene="cutA"
                     /locus_tag="VNN25_19520"
     CDS             complement(4626..4958)
                     /gene="cutA"
                     /locus_tag="VNN25_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXH93779.1"
                     /translation="MTPVIVLTTVAADFDARPLARSLVEQRLAACVNIVAGVTSIYRW
                     KGQVEEEGEQLLVIKTLEANVDALRNELLARHPYEVPEFLVLPVGSTSEAYGAWLLES
                     AGEDTRKT"
     gene            4985..5896
                     /gene="mtgA"
                     /locus_tag="VNN25_19525"
     CDS             4985..5896
                     /gene="mtgA"
                     /locus_tag="VNN25_19525"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXH93780.1"
                     /translation="MFRRILLIAFVALLLFVGYEWITFPDVASLAGTPPATTAFMEQR
                     KAELRAAGKPDAIDYRWVPYARISPYLRRAVLVAEDDTFFEHDAVDVKGLQEAIEKDW
                     AHKKLTHGGSTITQQLAKNLYLSPSRNPFRKVKEYFIARSLESHLTKKRILEIYINVV
                     EMGERVYGAEAAAQSYFHSAAASLSPSQAALLAACLPNPRVMNPASPNKRLRWRHRMV
                     LSRMRRWGYLFEKEVLTEKKPPPAAPVTETTATVDTALQQVPPTDTATPEPPTETTTT
                     APTSTDTAPPTETVPPPATTTTSPPKA"
     gene            complement(5972..6196)
                     /locus_tag="VNN25_19530"
     CDS             complement(5972..6196)
                     /locus_tag="VNN25_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXH93781.1"
                     /translation="MPINIKIRDNGPYLVEGEFTLTDAQGNPVEVKKAALCRCGGSTM
                     KPFCDGTHSKIGFKGANEAVQESDPAAAQS"
     gene            complement(6207..6569)
                     /locus_tag="VNN25_19535"
     CDS             complement(6207..6569)
                     /locus_tag="VNN25_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93782.1"
                     /translation="MRKSRRERGESQFGCLLGLILLGIAIFVAWKMIPVKVKAAEMRQ
                     TVVDEAKSAGTHNDERIKAAIMAKAREDALPLTEDDINIVRANGEITVTVNYTVPIAF
                     PGYTYQWHITHTATNPIF"
     gene            complement(6597..7199)
                     /gene="lepB"
                     /locus_tag="VNN25_19540"
     CDS             complement(6597..7199)
                     /gene="lepB"
                     /locus_tag="VNN25_19540"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXH93783.1"
                     /translation="MQSKSAIRLIVEPLAIAIALAFLVRATIRIYVIPSFSMAPTLMA
                     GDHIVVTPYRFRSEPQAGDVIVFRSPHAAGELMVKRVIGTPGDLVETREGRVIVRGHA
                     IAESYLAAQASTGLIPPQLVPANSYFVLGDNRTDSLDSRSWGLLPRADVLGRARLILW
                     SSEAPVGSRESGVVTATPSPTPHPPLPNPSRIDRLFKRIQ"
     gene            7454..7792
                     /locus_tag="VNN25_19545"
     CDS             7454..7792
                     /locus_tag="VNN25_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013057989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HXH93784.1"
                     /translation="MPIEPRQPQGLVDILDKILDKGLVVAGDIKVSLANVELLTIQLR
                     LVICSIDKAEAIGINWWRSDRFYSAAPNVAGSEEYDALKARVHELEQKLEARELPQST
                     QKKTDERKGR"
     gene            7796..7993
                     /gene="gvpJ"
                     /locus_tag="VNN25_19550"
                     /pseudo
     CDS             7796..7993
                     /gene="gvpJ"
                     /locus_tag="VNN25_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
     gene            complement(8155..8400)
                     /locus_tag="VNN25_19555"
     CDS             complement(8155..8400)
                     /locus_tag="VNN25_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93785.1"
                     /translation="MKLSRLVIALLIFTAIASGFIAGHAIADQPHMQSALEHLRLAKG
                     ELELADRDKGGHREKALRLTNDAIAETERGIAYDRHH"
BASE COUNT         1469 a         2555 c         2769 g         1740 t
ORIGIN      
        1 cctcggccac cacttcgccg ccgtgcactc cggcgcgcgg gccgaggtcg atgatgtggt
       61 cggcagtctc gatggtctcc tcatcgtgct cgaccacgat caccgtgttg ccgaggtcgc
      121 ggagctcgag gagagagtcg atgagcttgc ggttgtcgcg ctgatgcagg ccgatcgacg
      181 gctcgtcgag gacgtagagg acgcccatca gcttcgagcc gatctgcgtg gccaggcgga
      241 tgcgttgccc ttcgccgccg gagagcgtgg ccgcgctgcg gtcgagcatc aggtagccga
      301 ccccgacggc gcagaggaag ccgaggcggt cgcggacttc tttgaggatc ttgccggcga
      361 tcttctcttc gcgcggcgtg agatcgatcg tggcgaagaa gttctcggcg tctttgagct
      421 gcagaccgac gacctcatcg atcggctgcc cagccacggt caccgccagt gcctcgggtt
      481 tcagacgccg ccctttgcaa tcgggacaat ccatcggcgt catgaagcgc tcgatctctt
      541 cgcggatgtc gtcgttgtcc gattctttgt agcgacgcgt cagcatcggc acgaggcctt
      601 cgtacgttcc gctgtatgag taggtgctct ccgcgccgct gaactggaac ttgatcttct
      661 cctcggagcc ctgaaggatg acgctgcgcg ccttctccgg gagcgacttc cacggcacgt
      721 cgagcttgaa tttgtagtgc ttggccagcg tgtgaatctg gcgaagccgc cagttctcgg
      781 cgccctcctt ccagatggag atggcgccgt cttcgatcga cttcaacgta tcggggatga
      841 tcttcttctc atcgacctcg agcaggacgc cgaggccgga gcagcgcggg caggcgccgt
      901 acggcgagtt gaaggagaag agccgcggcg tgatctcgcc gatggagacg ccgcaatcgg
      961 gacaggcgta actctgcgaa aggagctctt cccggtttcc ttcctggtaa agaatcttga
     1021 ccaggccctt cgtcacgcgc gtggcggtct cgagcgaatc ggccaagcgc tgctcgatcc
     1081 cctctttgac gacgaggcgg tcgatgacaa tgtcgatggt gtgcttcttc tgcttgtcga
     1141 gtgtgggcgg gtcggccagc tcgatcatcg tgccgttgac gatggcgcgc gtgaagcctt
     1201 ccttcaccat ctgcagcatc tgcttgcggt actcgccctt cttgccgcgg atgtagggcg
     1261 cgaggatggt gaacttcgtc ccggtcttca gcgtcatgag ccggtcgacc atctgctgga
     1321 tcgtctgcgg gcggatctct ttgccgcagt tcgtgcagtg aggaacgccg accgaggcgt
     1381 agaggaggcg gaggtagtcg tagatctccg tgacggtgcc gaccgtcgaa cggggattgc
     1441 gcgaggtcgt gcgctgctcg atggagatgg ccggcgagag tccttcgatc gagtcgacgt
     1501 cgggcttctc catctgctcg aggaactgcc gggcataggc gctgagtgat tcgacgtagc
     1561 gccgctggcc ctcggcgaag atagtgtcga aggcgagcga tgatttgccc gatccggaca
     1621 cgccggtgat gacgacgaac ttgttgcgcg ggacgacgac gtcgatgttc ttgaggttgt
     1681 gctcgcgggc accgcgaatg acgatggatt ccatagggtc ggatcaactc taactccgcc
     1741 gcggcgaccc ctcttcccac gctacgtggg gtagagaatt tgacaaatgg tgccccttcg
     1801 cgttcttcgc ggcttcgcgt gatctctttt tctcacgcga agacgcgaag aagaccgaca
     1861 cttgacgctc acgctgcttg cgcgtatagc tccgcggctt ggcggccatc cttctctatc
     1921 tcgcgacgac cgtaggcgtg atcgtgcttt ggcggcgatt cgtgcagccg atctcgcgca
     1981 tggccgcgct cgtcctgatc ctgctgccga tgtgctttac gggaagggct ttgctcaccg
     2041 gacgggtgta cgcgccgatc gatctgccgt tcatgtcgga gccgctgaat gcgtatgcgg
     2101 cagagttcaa cgtgccgaag gttcacaacg gcacgctttc cgacctctac tgccagatca
     2161 ttccgtggcg cagcgcggtg cgaacggcga tcttcagcgg gcagtggcct ctgctcaatc
     2221 cgttcatgct ttgcggcgac atcctggcgg cggcggcgca accggctcct tacgatccct
     2281 tcaacctcct cggcatggtg attccgatgc cgccgagtct gacgttcgcg gcggcgatga
     2341 cgttcttcct ggccggcttc ggtgcgttcg ccttcgcacg tgcgctcggg tgcggggagc
     2401 tggcgtcgct cgtcgccgcg gcggggtgga tgttcagcgc caacatcgcc ttcttcgtcg
     2461 gatggccgct ttcgcgatcg tgggcgtatc tgccgctgat tcttttcgca gtacagcggc
     2521 tggtgaacga agcgagcgtg cgtgccgggg ttctgctgat gatcgcactg acgtccttga
     2581 tcctcgccgg gcatccggag tcgttgctgc acgtcaccgc ggtcggtgct gcctatggtc
     2641 tgttcgagat tgcgacgaca cgaaaacggg tcggacgcgt gatcgcgctc gccgccggcg
     2701 cgggcgtcgt ggcgctgctg ttgacggcga tctttctgct gccgttcctc gaggcgtcgc
     2761 aacaaacgct cgagcttcac aaccggctcg gctaccgctc gatgtcgttt ccgttcatgc
     2821 cggagatgat tcagcgtcgc gcggccaaca ccgtctttcc gttctggggc gggcagccgt
     2881 ggcgcgattc gtttacggcc gaatgggatc cgcagaccgc gcgcacgggc agcatcgtcc
     2941 tggcgctggc gctgaccgca ttgatcgtgg cgaggaagcg caaagagacg tggttcttct
     3001 tcgcgctggc gctcatttgt ctttgcgcag gtttcgacgc gccgccgatg gcgaacgtgc
     3061 tccacaagat cccgctcttc gatgtggcgc tcaacgaacg gttggcgtac gcggcgtcgt
     3121 gtgcgttggc gattcttgcg gcgctggcgg tagatgcgtt cgcaaccgag gttggcggct
     3181 ctacacgtcg tagggtggcg gcggtggtgg ctggcgggat cgcaatcgtc ctcggcgtcg
     3241 gaacgtggct ggtctgggcg cacgagctca aagtcggcgt cgatccgtcc gtcatccgca
     3301 tcaacgcgct cggcgatctg attccgctgg tgatcgtcgc catcctgctc gcttcacgca
     3361 ccccgcttcg catcgccatc cctctcgttc tcgttctcct cctcggcgca cgcatcatcg
     3421 tcgacggttc gatgtatccc tcgctgccgc agcacgattt ctatccgaag atcccgatca
     3481 tccgcgccat ccctgagaac actccggagc cgttccgcgt ggccggcgag catttcgtgc
     3541 tcatccccga ttcggcggcg atctaccagc tcgaagaccc tcgcggctac gaagcgatga
     3601 cgttcgcgcg cctcgccgac acctacccgg tctggtcgat cccgcagccg gtgtcgttca
     3661 acgccatcgg tgatcttcaa ccgccgttcc tgtcgttcct caacatcaag tacgcgatcg
     3721 tgccgatcag cgttgacctg actccgggct ggcgcgaatt gatgaaggac cgcagcgcga
     3781 agttgttcga gaatacgcac gttctgccgc gcgcgttcgt gccgcgccgc attcgttacg
     3841 agcgggacgg cgttctggcg ctgaaggcga tgaggacggc gaaggatttt tcggacatgg
     3901 cctggatcga ggtgccggag gacaagcctc acgaaatagc gaacggtccc ggcacgctga
     3961 cgcttcaccg cgacggtttc ggcctcgaca tcgacgccac gatggccgct gacggctggg
     4021 tggtggcgtc gcagtccgcg tggaaaggtt ggcgcgccta catcgacggc cgccgcgtcc
     4081 agatgcgctt cgccaatcac gccttcctcg gcgtcttcgt tccggccgga cgacatcacg
     4141 tgaagctgca gtacctgccg gaatcgttta cgcgaggacg ggcgatttca tttgcaacac
     4201 tcggactcct gattgttctt gccggcggga cgacattgcg gcgaaagaga tcagagacaa
     4261 gagcagagat tagagacaag agattggaga cgagatcaga gactgacgcg tcatccggag
     4321 tgatcgtcta atcgagattg gtgtcatcct ggtgtcatcc atggtgtcat ccatggtgtc
     4381 atcctgagac tgccggagcc gcccgcgcga atcgtgatgt ttgcgcagta cgtgctcggc
     4441 ggcgtaggac gtcgaaggat ctcaaacttc gcagatcgtg tttgtccagc gcgagcgatc
     4501 gtgttcaaag ccgctcgaac gagatcttcg cattttgaga tccttcgacc gtcctacgcc
     4561 gccgatgtct gccggttgcc ggtgcagcga aacttgcggc ggcttcggcg gctcaggatg
     4621 acacatcacg ttttgcgcgt atcttctccc gccgattcga gaagccaggc tccgtacgct
     4681 tcggacgttg accccaccgg cagcacgaga aactccggaa cctcgtacgg atgccgggcc
     4741 agcagttcgt tgcgcagcgc atcgacgttc gcttcgagcg tcttgatgac gaggagttgc
     4801 tcgccttcct cctcgacctg acctttccag cgatagatcg acgtcacgcc ggcgacgatg
     4861 ttcacgcagg cggcgaggcg ctgttcgacg agagagcgcg cgagcggacg ggcgtcgaaa
     4921 tcggcggcga cggtggtgag aacgatgacc ggggtcatga ccgcgcatca tagaatcggc
     4981 cggcatgttt cgccgcattc tgcttatcgc cttcgtcgcg ctccttctct tcgttggcta
     5041 cgaatggatc acgtttcctg acgtggcttc gttagctggg acgccgccgg cgacgaccgc
     5101 gttcatggaa cagcgcaaag cggaactgcg tgcggccggc aagccggatg cgatcgacta
     5161 tcgatgggtc ccgtacgcgc ggatctcgcc gtatctgcgg cgcgctgtgc tggtggccga
     5221 ggacgatacg ttcttcgagc acgacgcggt ggatgtgaag ggactgcagg aggccatcga
     5281 gaaagactgg gcgcacaaga aactaacgca cggcggctcg acgatcacgc agcaactggc
     5341 gaaaaacctc tacctctcgc cgtcgcgcaa tccatttcgc aaggtgaagg agtacttcat
     5401 cgcccggtcg ctcgaaagcc atctgacgaa gaaacggatc ctggagatct acatcaacgt
     5461 cgtggagatg ggcgagcggg tttatggcgc cgaggcggcc gcgcaatcgt atttccactc
     5521 ggcggctgca tcgctttcgc cgtcgcaggc cgccctcctt gccgcttgtc ttcccaatcc
     5581 tcgggtcatg aatccggcca gtccgaacaa acgtcttcgc tggcgtcata gaatggttct
     5641 ctcgcggatg cgccgctggg gttacctgtt cgagaaggaa gtgctgacgg agaagaagcc
     5701 accgccggct gcaccggtga cggagacgac cgcgacggtc gatacggcgt tgcagcaggt
     5761 tcctccaacg gacacggcaa cgccggagcc tcctacggag acgacaacga cggcgccgac
     5821 ttcgacggac accgcgccgc caacggagac cgtaccgccg cctgcgacga cgactacgag
     5881 cccgccaaaa gcatgaacca cagagacaca gagatcacag agaaaacctc tgtgtcctct
     5941 gtgtctctgt ggttagaagg tttgtcgtgt ttcaggattg agcggcggcg gggtcgcttt
     6001 cctgtactgc ttcgtttgcg cccttgaagc cgatcttcga gtgcgtgccg tcgcagaacg
     6061 gcttcatggt cgaaccgccg cagcggcaga gtgccgcttt cttcacctcg accggattgc
     6121 cctgggcgtc ggtgagggtg aactcgcctt cgacgaggta gggtccgttg tcgcggatct
     6181 tgatgttgat gggcatgagg gtccttttag aaaatggggt tagtcgcggt atgcgtgatg
     6241 tgccattggt aggtgtagcc ggggaaggct atagggacgg tatagttgac ggtgacggtg
     6301 atctcgccgt tcgcgcgcac gatgttgatg tcgtcttcgg tgaggggcaa ggcgtcttcg
     6361 cgcgctttgg ccatgatcgc tgccttgatg cgctcatcgt tatgcgtgcc agccgatttg
     6421 gcctcgtcga cgacggtctg tcgcatctcg gccgctttca ctttcacggg gatcatcttc
     6481 caggctacga agatggcgat tcccagcagg atcaacccga ggagacatcc gaactgactc
     6541 tcaccgcgct cgcgacgact cttgcgcata aaaacctccg tcggcgctga gtatacctat
     6601 tggatgcgct tgaagagacg gtcgatgcgg gaggggttgg ggagtggggg gtggggggtg
     6661 ggagaaggag tggccgttac gactcccgac tcccggctcc cgaccggagc ttccgacgac
     6721 cagagaatca atcgcgcacg gccgagcacg tccgcgcggg ggaggaggcc ccagctacgg
     6781 ctgtcgagcg agtcggtgcg gttgtcgccg aggacgaagt aggagttggc ggggacgagt
     6841 tggggaggga tgaggcctgt gctcgcctga gcggcgagat acgactcggc gatggcgtga
     6901 ccgcgaacga tgacgcgccc ttcacgcgtc tccacgagat cgccgggcgt gccgatcacc
     6961 cgcttgacca tcaactctcc cgcggcatgg ggggatcgga agacgatgac atcgccggcc
     7021 tgcggctcgc tgcggaaccg gtacggcgtg acgacgatgt ggtcgccggc catcaaagtc
     7081 ggagccatcg agaatgaggg aatcacgtag attctgatcg tcgcgcgcac gagaaacgcc
     7141 agagcgatgg cgatggccag cggctcaacg atgaggcgga tcgcggattt ggattgcact
     7201 ggggtgttca acgagggaga tggctgcgtc atttcgtttg cgggtgtcgg gggtcggggg
     7261 tcggagctgc gggtgtcgcg gatcgggggt cggcagtcgg agtgggttca tcgcgggctg
     7321 cgccgcggtc gctcgcgtac cacagatggc gagctaccga ttccactccg acccccgacc
     7381 cccgaaacgc gacccccgcg cccgaccctc gcagtcttga cgtgcctcct ccgcgcgaat
     7441 actgttcgcc cacatgccga tcgagccgcg acagccgcag ggacttgtcg acatcctcga
     7501 caagatcctg gacaagggtc tcgtcgtggc cggagacatc aaagtctcgc tcgcgaatgt
     7561 agagctcctg acgatccagc tccggctcgt catttgctcc atcgacaagg ccgaggcgat
     7621 cggcatcaat tggtggcgct cggatcggtt ctactccgcg gcgcccaacg ttgccggcag
     7681 cgaggaatac gacgctttga aggctcgcgt gcacgagctc gagcaaaagc tcgaggcgcg
     7741 ggagcttccg caatccacac agaaaaaaac agacgagagg aaaggcaggt aagtcatggc
     7801 agtcgaacgc gcaccaggcg gcaccagtct catcgacgta ctcgaccgga ttctcgacaa
     7861 aggtatcgtc gtcgatgcgt gggtcaggat ctcgctcgtc ggcatcgacc tgatcacggt
     7921 tgaagcgcgc gtcgtcatcg cttcgatcga tacctatctg aagtacgccg aagcggtcgg
     7981 tatcacctcg accgtcgccc gaccggccct cggcccgggt gagggcggca caggtggcgg
     8041 aaccggcagc atgacgccgg cttcaggcac cggccgcgca taaaacgcgt ccgctctcgc
     8101 cgagcaaaca acgcctgccg cgcagagcgc acggcaggcg ttttcgtttc gctctcagtg
     8161 gtgccggtcg taggcgattc cgcgctcagt ttcggcgatc gcgtcgttgg tcagcctgag
     8221 ggccttttcc cggtgaccgc ctttgtcgcg atcggccagt tccaattccc ctttggccag
     8281 acgcagatgt tcgagcgctg actgcatgtg gggttggtcg gcgatggcgt gtccggcgat
     8341 gaatcccgag gcgatggcgg tgaagatgag aagggcgatg acgagacggc ttagtttcat
     8401 gggatctcct ccttgtcgat aatcgacgcg gcatgcgtcg aagcgtttac cggtggggtc
     8461 cccctgtagg aatcaaccga ttaatggatc agtcgatgag cggtcagtga tcagcaaaaa
     8521 aagtgcctga cca
//
LOCUS       DATLVW010000672         4394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_154825, whole genome shotgun sequence.
ACCESSION   DATLVW010000672 DATLVW010000000
VERSION     DATLVW010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154825"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..2198
                     /locus_tag="VNN25_19560"
     CDS             81..2198
                     /locus_tag="VNN25_19560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH93786.1"
                     /translation="MKRLLALLLLLAASAYARNITEKDLFKFNWIGDPQLSPDGSEVA
                     FVRVTVDEKKDTYNTAIWAVAAHSGAEPRRITNGPRDASPRWSRDGKLLAFTRSIDKD
                     GKPQPSQVWVLRFDGGEPHVLTALPKAADSIAWSPSANTIAFTATTKPDDLDKKDDKK
                     KDDEHESDVRIINQAVYRANGAGYRDASRDTHIWTIDVPDTLGSDEALPKPKQLTSGE
                     FDERDVTWSPDGSQIYFTSNRVRESYFDRRDTDLYAIPAGGGEMKKVADIEGPIGSYA
                     VSPDGKWIAFAGEINNPVHSYDQPDLWLVATAPGSTARNLTATYDYDILSGVGGDQRA
                     PRASGGGARPVWSDDGKTIYAVSAEEGRVNMKRIDAATGKITPWTSGNHDIQSYSIRG
                     GKTVALISTPTIIGDLYLVAGDGAMTRLTNINDKLMREVKLTEPEEIWYTSFDGKKIQ
                     TWVQKPPDFDAAKKYPLILNIHGGPHSAYGYTFDHEFQWMAAKGYVVLYPNPRGSTSY
                     GQDFGNIIQYHYPGDDFKDLMAGVDAVVARGYIDPKKLCVTGGSGGGLLTDWVVTQTD
                     RFAAAAAQRDIADWSTFWYVADFSQFQPSWFRKAPWEDPKDYVMRSPITYIDRVKTPL
                     MLILGEADYRTPPMSGGEMMFRALKYRHIPTVMIRFPDESHELSRSGKPWHRIERLEH
                     IVGWFDHWVMGVPKPEYEEGLRK"
     gene            complement(2582..3571)
                     /locus_tag="VNN25_19565"
     CDS             complement(2582..3571)
                     /locus_tag="VNN25_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXH93787.1"
                     /translation="MKTFLKIVGGLVLIVIALIAGVVIWLSARKPASRPASTEKIEAT
                     PERLARGTYLVEHVADCLGCHSDHIIKFGIPVKPGTEGEGGYIFDEKIGFPGVVAAQN
                     LTADQEFGLGKWTDGEIIRAIREGIDRNGEALFPMMPYEHMRSMSDDDVKSVVVYLRT
                     LKPIHKGVPAKHINFPVNLFVKFVPKPVDGVVPTPDPHDNLAYGKYLSTIGGCYECHT
                     PHDDKGTRIADKAYSGGWEMKGPWGRNFTANLTSTPGTYMAHATKEEFIGRFKAFASM
                     NANTAPDAPAGRNTVMPWLAFSQMTEQDLGALYDYLKTVKPIENKVVTFPDAK"
     gene            3638..>4394
                     /locus_tag="VNN25_19570"
     CDS             3638..>4394
                     /locus_tag="VNN25_19570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH93788.1"
                     /translation="MIASLLLAATLQTNVADVTKVYERDYAPRLVPLLSEVLRFPTTQ
                     FDTDGHAAQKEWIAKTAHDLGLTYRDAGLIDEIELTGPAGAPVLGLMVHGDVQPVDAD
                     AWSVAPFVGKADDTFVYGRGSADDKGPLVQALLAMKALEQSGIKRTHTVRLLIGSDEE
                     STNTDVTTYLKSHQAPDYTLVLDSGFPVIVGEKAWNSLSVTTDALAERGTSSKPYAVS
                     YLDAGLGTSIVPERAEVKLKWKGTGVNWQPVIDA"
BASE COUNT          855 a         1383 c         1368 g          788 t
ORIGIN      
        1 acgcggagac gcggaggacg cggcgggttt ctccgctcct ccgcgcctcc gcgtgacagg
       61 tccctcgtac aatttccgac atgaaacgcc tgcttgcgct gctgcttctt ctcgccgctt
      121 ccgcatacgc ccgcaacatc accgagaaag atctcttcaa gttcaactgg atcggtgatc
      181 cgcagctctc gccggacgga tcggaggtcg cattcgtgcg cgtgaccgtc gatgagaaga
      241 aggacaccta caacaccgcc atatgggcag tcgcggcgca cagcggagcc gagccgcggc
      301 ggatcacgaa cggtccgcgc gatgcctcgc cgcgctggtc gcgcgacggc aagctgctcg
      361 cgtttacgcg ctcgatcgac aaagacggca agccgcagcc gtcgcaagtc tgggtcctgc
      421 gcttcgacgg cggtgagccg cacgtattga ccgcgctgcc gaaggcagcg gattcgatcg
      481 cctggtcgcc gtcagcgaac acgatcgcgt tcacggccac gacgaagccg gacgatctcg
      541 ataagaaaga cgacaagaaa aaggacgacg agcacgaaag cgacgtgcgc atcatcaatc
      601 aggctgtcta ccgtgcgaac ggcgccggct accgcgacgc gtcgcgcgac acgcacatct
      661 ggacgatcga cgtccccgac acgctcggca gcgacgaagc ccttccgaaa ccgaagcagc
      721 tcacgtcggg cgaattcgac gagcgcgatg tgacctggag tcccgacggc tcgcagatct
      781 acttcacctc gaatcgcgtg cgcgagtcgt acttcgaccg ccgcgatacc gatctctacg
      841 cgatcccagc cggcggcggc gagatgaaga aggtcgccga catcgaaggt ccgatcggct
      901 cgtacgccgt gagccccgac gggaagtgga tcgccttcgc gggcgagatc aacaatccgg
      961 tgcattcgta cgaccagccc gatctctggc tcgtggcaac ggcacccggc tcgaccgcgc
     1021 gaaatctcac cgcaacgtac gactacgaca ttctctcggg cgtcggcggc gaccagcgtg
     1081 cgccgcgcgc aagcggggga ggagcgcgtc cggtctggag cgacgacggc aagacgatct
     1141 acgccgtcag cgccgaagag ggccgcgtca acatgaaacg catcgacgcc gccaccggca
     1201 agatcacccc gtggacgagc ggcaatcacg acatccagag ctactcgatc cgcggcggca
     1261 aaaccgtcgc gctcatctcc acgccgacga tcatcggcga cctctatctc gtcgccggcg
     1321 acggtgcgat gacgcgcctc acgaacatca atgacaagtt aatgcgtgag gtgaagctca
     1381 ccgagccgga agagatctgg tacacgagct tcgacggcaa gaagatccag acctgggtgc
     1441 agaagccgcc cgacttcgat gccgcgaaga agtaccccct catcctcaac atccacggcg
     1501 gtccgcactc agcgtacggc tacacgttcg atcatgagtt tcagtggatg gcggcgaagg
     1561 ggtacgtcgt cctctatccg aatccgcgcg gctcgacgtc gtatggccag gatttcggaa
     1621 acatcatcca gtaccactat cctggcgacg acttcaaaga cctgatggcc ggcgtcgatg
     1681 cggtggtcgc gcgcggctac atcgatccga agaaactctg cgtgaccggc ggaagcggcg
     1741 gcggtctgct caccgattgg gtcgttacgc agacggaccg ctttgccgcg gccgccgcgc
     1801 aacgcgacat cgccgactgg tcgacgttct ggtacgtcgc cgacttctcg cagttccaac
     1861 cgtcgtggtt ccgcaaagcg ccgtgggagg acccgaagga ttacgtgatg cggtcgccga
     1921 tcacctacat cgaccgcgtg aagacaccat taatgttgat cctcggggag gccgactacc
     1981 gcacgccccc gatgtccggc ggcgagatga tgtttcgcgc cctcaaatac cgccacatcc
     2041 cgaccgtgat gattcgcttc cccgacgaat cgcacgagct ctcccgcagc ggcaaaccat
     2101 ggcaccgcat cgaacgcctc gagcacatcg tcggctggtt cgaccattgg gtgatgggcg
     2161 tgccgaagcc ggagtacgag gaggggttga ggaagtgatt cggcgtgggg tgtggggagt
     2221 ggggagtagg gtgtgggaga aaccgaatcg gcccgtgtca tcacgccggt ttcgtgatgg
     2281 aaattgatgt gtcatcctga ggcagccgga ggcgcccgca cgcctcgcgc cttcacgcag
     2341 gatgggacgg cgccgcagga cggcgaagga tctcaagtta ccgggcacat gactcatgcg
     2401 tcgtaatttg agatccttcg acgtcctgcg ccgccacgtc ttgcagcgca acggctcgag
     2461 tacggatggc ggctccggcg gtctcaggat gacaggatcg ggtgaggccc gaagaccgga
     2521 gcacggacag aatccgattg tcctactccc actccccacc ccaacacccc accccccgca
     2581 atcacttcgc gtccgggaac gtaaccacct tgttctcgat cggcttcacg gtcttgaggt
     2641 agtcgtaaag cgcgccgagg tcttgctcgg tcatttggga gaaggcgagc cagggcatga
     2701 ctgtgttgcg tcccgcgggg gcgtccggtg ccgtgttcgc gttcatcgaa gcgaaggcct
     2761 tgaagcggcc gatgaactcc tccttcgtcg cgtgggccat gtatgtcccg ggcgttgacg
     2821 tcaggttcgc ggtgaagttg cggccccacg gtcctttcat ctcccagccg ccggagtacg
     2881 ccttgtcagc gatcctggtc cctttgtcat cgtgcggcgt gtggcactcg tagcagccgc
     2941 cgatcgtcga cagatacttt ccgtaggcaa ggttgtcgtg cggatcggga gtcggcacca
     3001 cgccatcgac cggcttcggt acgaacttca cgaagaggtt gacggggaag ttgatgtgct
     3061 tggccggaac gcctttatgg atcggcttga gcgtgcgcag gtagaccacg accgatttca
     3121 catcgtcgtc gctcatgctg cgcatgtgct catacggcat catcggaaac agtgcttcac
     3181 cgttgcgatc gatgccttcg cgaatggcgc gaatgatctc gccgtccgtc cacttgccca
     3241 gaccgaactc ctggtccgcg gtgaggttct gcgcggcgac gactccgggg aagccgatct
     3301 tctcgtcgaa gatgtatccc ccttcgccct cggttcccgg cttgacggga atcccgaact
     3361 tgatgatgtg gtcggagtgg cacccgagac aatccgcgac gtgctcgacg agatacgtgc
     3421 cgcgcgcgag ccgctccggt gtggcctcga tcttctcggt tgacgccggg cgcgatgccg
     3481 gcttccgcgc cgacagccag atcacaactc cggcgatcaa tgcgatgacg atcaacacaa
     3541 gtccacccac gatcttgaga aacgttttca ttggtgcttc ctccgagttg gaacgattga
     3601 tcccatttta cgggagaatt cgcatatgcg tttcccgatg attgcctctc tgctcctcgc
     3661 cgccaccctg cagaccaacg ttgcggacgt gacgaaagtc tacgaacgcg actacgcgcc
     3721 gcgcctcgtg ccgttgttgt cagaagttct tcgctttccg acgacgcagt tcgacacgga
     3781 tggtcatgcc gcgcaaaaag agtggattgc gaaaacggct cacgacctcg ggctgaccta
     3841 ccgcgacgcc gggctgatcg acgagatcga gcttaccggt ccggccggtg cgccggttct
     3901 gggactgatg gtgcacggcg acgtgcaacc ggtcgatgcc gacgcgtggt cggtcgcgcc
     3961 gttcgtagga aaggctgatg acacctttgt ctatggccgc ggatcggccg acgacaaggg
     4021 tccgctcgtg caggcgctgc tggcgatgaa ggctctcgag cagagcggca tcaaacgcac
     4081 gcacacggtg cgtctgctca tcggaagcga tgaggagtcg acgaataccg atgtgacgac
     4141 gtatctcaaa tcgcatcagg cgcccgacta cacgctcgtc ctcgactccg gatttccggt
     4201 catcgtcggc gagaaagcgt ggaactcgct ttcggtcacg accgatgcgc tggcggagcg
     4261 cggcacctcg tcgaagccgt acgcggtgag ctacctcgat gccgggctgg ggacgagcat
     4321 cgttcctgag cgtgccgagg tgaagctgaa gtggaaggga acaggagtga actggcagcc
     4381 ggtgatcgat gccg
//
LOCUS       DATLVW010000673        23345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_439331, whole genome shotgun sequence.
ACCESSION   DATLVW010000673 DATLVW010000000
VERSION     DATLVW010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23345
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439331"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VNN25_19575"
     CDS             complement(<1..688)
                     /locus_tag="VNN25_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXH93789.1"
                     /translation="MPPPILARAGSRIADVCERWFPDAFVFALAAIVLVFFAGLGWGV
                     KPVTLATEFGNGFWSLVNFTMQMAIVIVGGFVVASSPPVAALTRRLAAIPKTARGAVA
                     FVAAFAMLSSLLSWGLSLVFAGLLVREITKRVDADYRAIGAAAYLGLGSVWALGLSSS
                     AALLQATKSSIPPALLPITGVIPLTQTIFLWQSMVMAAVLVIVSIAVAFFSAPAETEA
                     RTARAMGIELA"
     gene            complement(800..1315)
                     /locus_tag="VNN25_19580"
     CDS             complement(800..1315)
                     /locus_tag="VNN25_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93790.1"
                     /translation="MTPTETVGLIVGLIAIIEAGYVSGKWRASKQRHAMLDEAVAARI
                     RQLLGAVEIRGLTSNSQEASPGQTIRISYDVFSKAEFPYQVWLGASILAADGTEYFDQ
                     SQDKEITLEPGLTNQSRYLTVPGDVLAGSYNLYGAIWLGARAITENSITLARMKSSSP
                     LLVRGNVQRTV"
     gene            complement(1539..2231)
                     /locus_tag="VNN25_19585"
     CDS             complement(1539..2231)
                     /locus_tag="VNN25_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93791.1"
                     /translation="MSKKYDPPKPGVGDAAQAITKAAVSAVPLLGGPAAELVNAVFTP
                     MLERRRQEWMEQVAEALRTLEDKRGIPIEELKNNEVFGDTVLQATQIALRTSQDEKRA
                     ALRNAAINAALPTAPAQSLQQMFLSFIDVFTVWHIRILKLFDAPEKNTVLVGARWPNL
                     MMGGMAYILENAYPKIAGNRGFYDQVWRDLYSRGLVNTESLHSTMSAQGLAEQRTSGL
                     GREFIEFISEPV"
     gene            complement(2308..5343)
                     /locus_tag="VNN25_19590"
     CDS             complement(2308..5343)
                     /locus_tag="VNN25_19590"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH93792.1"
                     /translation="MRRPSFVLLALLVLVVALPLLAQSQATTGVIEGTVVDASGGAVP
                     GVTVTVKNTGTGYESISVTDSGGRFRAVLLPVGPYQLSAALQGFATVVQKGLALGVGQ
                     TLTVPVVLKQAATAEEIVVTAAPPLIETARTEGATRVDEKAMEGLPNNGRNFLEFTKL
                     TPGVTIVQGPDGDELSINGQKGISNNVSVDGADFNNPFFGEQRGGQRPPFTFNLDAVK
                     EMVVISDGAGAEFGRSMSGFVNVITKSGTNDSAGTAHVTFKNDSLSSQPKNPDGTTSP
                     KFPSSQTQAGFTLGGPLQRDKLFYFAALDYQKGKTTKQTNPGRIEQRVVDALASLGSP
                     NENGSIARTNDARVLLLKLDWNASAKNLANLRYNYTWSQQANGTFDVDSWGRSANAIE
                     KDSSESVVGGLTSTLQSSTFNEFRFQAAKENRPRPYNGPNITGQNRPLPDTAFDFAKS
                     YRFGEPFFIPVKYYDTRVQFNDNVNFLRGAHDIKAGLEFNRVNSVQTFLGFANGRYIF
                     SSTDGFLNYLKNPNYVECADAQNNFASSQTGVCPAGFNITGPVLLYLQQAGVGNISVE
                     QAGTQSIPQTEPAVFVQDSWQATPHLTVNYGLRWEAEIEPDPITPPDQVFYAGFIGKT
                     SKGQEFPSNGKIPSDRKMWQPRLGLSWDPRGDGKEVLRASAGIQYGRVPGLDLASSRS
                     TNGSRGQTIFRNSALTGILGPVPAYPNIIPQSQVGSPFDPDVFVFDKHFQNPRTQQAS
                     VSWEQEVVKDYGFLVKYNYIKGDHITRFINRNDPLLGSPWSTGLGAGGANGIGALTTI
                     ESSAKSLYNGVTLGINKRPSNNLQFEVNYTYSKDKSDDDNERDPFTFRYAKVTDLAAE
                     YGYSDRDQRHRLNSWLLWNAPMGVDVNVRYSYRSAQPKSLSCVVSVAFCGKDAFGASR
                     FMAEAQTPQDRINPDGSVTQRNLGRKDNVYSSLDLRFTKDFPFGAYKVEPAIDIFNLL
                     NSKNFHRPEVTNLIFNFDGTVQSGVGEPRQMQLGVRVIW"
     gene            complement(5454..6050)
                     /locus_tag="VNN25_19595"
     CDS             complement(5454..6050)
                     /locus_tag="VNN25_19595"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXH93793.1"
                     /translation="MTAKLPRSFYLQDTVTVARSLLGCVLWRRIGRALLAARLVEVEA
                     YLGANDSASHARRGLRSARNESMYLEGGHAYVYFTYGMHWCLNVVTAEGDIAEAVLLR
                     AAEPLRGIEAMRERRPKAKRDRDLMSGPAKLCSAMGVGKSLDGEPLDGNALWLTGRDH
                     VVDEDDIAVSHRIGIENSGEAARWPLRFFIRGNPYVSR"
     gene            complement(6047..6892)
                     /locus_tag="VNN25_19600"
     CDS             complement(6047..6892)
                     /locus_tag="VNN25_19600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXH93794.1"
                     /translation="MTTHSNDAELAAQAATLGAAILLKYWEHLGKDDADLKSRNDWVS
                     RADRESEAAIVAFIREHFPHDAILGEEGGISAEGTSGRTWIIDPLDGTSNYLQHFPVW
                     SVSIGLKTGDEITAGVVHEPLRNLFFTAERGAGAFRNGERMHVSSQATVEGAFLATGF
                     PFRAQQFVSVYTEIFREVISVSKGVRRAGSAAIDLAYTAAGIFDGFFELHLSPWDVAA
                     GSLLVTEAGGVFSDFSGGNRWLERGNIIGAPPGVHADLVRLINRFVSEDQLDRRSSNR
                     ALPSA"
     gene            6999..9398
                     /locus_tag="VNN25_19605"
     CDS             6999..9398
                     /locus_tag="VNN25_19605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH93795.1"
                     /translation="MQPPRISRYRVGRLIGAGGMGEVYLAEDETLNRKVALKLLPVRF
                     TQDEERVRRFQREARAASALNHPNILTIYEIGHTDSLHYIATEYIEGETLRDRMARQR
                     LKMAEVLDVATGVASALAAAHDAGIIHRDIKPENIMVRPDGVVKVLDFGLAKLADPES
                     ALKESSSGGIMGTLLYISPEQASGLQPDARSDLYALGAVMYEMITGHAPLRGESFIDI
                     AFAIAKEKPKPPSSSVDGVPPELDSIILKLLEKDRDRRYASARQLLGDLNTLRRELEF
                     ENKLNSLEMQRHVSAPGFAQQPTAQMTFPINTRARVRRAFRDRRVVVPLAFLTVALLA
                     GAAYMIMKRGIFADRIDSVAVLPFVNVSGDPNTEYLSDGLTDSIMDSLSQLPEIQVVA
                     RYKGKGSDPLAVGRELHVRGVITGRLIQRGNTIVIRASLTDVTRGTQVWGQQYDRSLS
                     DVIALQKDLSEEISSQLQPHLTGEERKLMSRSRPESNEAFQLYLKGHYYATKFNDDES
                     IRKGIAYFNQSIERDPTYALAYAGLSAAYYQLSNLFMPPREAMPRVREAAEHALALDQ
                     SLAQAHMCMALTLTWYDWDFVNGEKEFRRAIALNANDSEAHKAYGDLLGAMGRFDEAI
                     VEKRRAEQLDPLGVSTSWDAGRILFYAGRDEEAIEQAKRALDLDSKFPYIYDLEAQIA
                     YRKGNLPEALSLMQQALQFGGHKQLLITDWGMLNARAGRRDVALGAMNELRDRATYTL
                     PLFLARIYAALGNNDEAMKNLEKVYNDRSESAVWLKVDPSLETLRKDPRFIALIRKVG
                     L"
     gene            complement(9523..9741)
                     /locus_tag="VNN25_19610"
     CDS             complement(9523..9741)
                     /locus_tag="VNN25_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93796.1"
                     /translation="MSVTDTSPKVAAIHLELYRAVGPARRAQIAADLSDAVRETTLAG
                     IRRRHPELSEDDIRESFLQLVYGFGKRR"
     gene            10349..14269
                     /locus_tag="VNN25_19615"
     CDS             10349..14269
                     /locus_tag="VNN25_19615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXH93797.1"
                     /translation="MRGTARRARSAITIDLREFGWDRALIATMRHSTLPWFDGCVPVV
                     SLDATPLAEVAEFGPRDRLSLVAQFSAHQALLHFAGFSDAELDPAEWAVVRKRGNDCR
                     LVRTAARTTADEAPALTVIQQFAEVVSAPPLDSLRQSWGRAESVYLEAEARLRGDAAA
                     DLQWMRRSAFGAISAPGADALREMLRSGVGRFRASDAIDSIRVAAALGSEQIIAIGPG
                     ASPLQRYSALRALAPITGAIERCGESQIVERVVEAASRQHLIFSVTNRDSFDASSARV
                     IEMLAATDAGIWIIDDGNESDLPPSRFFVVAPFLAARRDIEERFAAFTAPDARSWLEH
                     FVESAAFARFLDTGTLPEEERATSVSSLREPARSCIAALALLGTRVRIDIASAFFQQL
                     MFGGVAGELAIEGITSIEDGHIVFASDAIRVAAKNLIPSSSRPMLSRVAAGVMAGTAG
                     DVSGAAALLIDAGDPLAAAELLEQVTVTGDQHLLLLRSLPRQALTPKLARTLASALIE
                     RGRYRDARDIVLLLTEEVRELFLARIERRLGDYSTARSRLERVTKRTFDHDALRIELL
                     HLESRYDDMRDALAACTAPQGEEERARLAYLRALHAHETGAQTDPKDDAELPPDHYFA
                     SRLATYRATSRQDFDDAAGHAAKSFARARDAAERIDAALDRVYILFTAGAWQEAREEA
                     LRTLALVEETQGDPAAGGILFILAYLAADDGQWAHAAQHIHRLRNFFRVTSDERRLLE
                     TELLSAHLDFSRGRFSDAMRSAQFIADSNFTAQMREAAALILDEIDWMESRETPLRST
                     GTSGNVELTRRNEMLRIRRSGGHVDLSGAINRSQRLQLLRDAIGCGRSEEAASIAAEM
                     GVELPVGEGNASSLELRMLRDAALREFPFGPRDFVPTPWRFATRNRLGQWHEIGSLSP
                     AHATELDRVLASAATDWIICSDRELLFVDGISRWCAESREALAAIFRTRAGHHRMQRV
                     IEQNENVTVAKSEAIDGIIGDSPAMRELGSLIIRVARRDVPACILGESGTGKELVARA
                     IHRYSPRRGKTFTAVNCAALPENLIESELFGCVRGAFTGADRDRAGLIETSEGGTLFL
                     DEIGEMPLVAQAKLLRFLQEGEFRRVGDTVNRTADVRIVSATNRKLEAAVEEGRFRED
                     LYYRIGGVEIVLPPLRDRVTDVAMLAAHFLAKEQEKSRGGPARLTADAESVFLAYRWP
                     GNVRELQNTIRAAHALAGDAAAIDVEHLPERMRSAVVRGGAGISSYQDAVTRFRRDLI
                     EKSLLQANGNQNRAAASLSMSRQALAYQIRELGILVRPHVVTER"
     gene            14322..15686
                     /locus_tag="VNN25_19620"
     CDS             14322..15686
                     /locus_tag="VNN25_19620"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HXH93798.1"
                     /translation="MRHLPAIAAVAVVAGCALYSDVSISPLSLAPGAIDQGSDLPSML
                     RKSDYLRAVEQASLVDAKTKRNPAELVSLGSAELAAGQFDVARRHLRAALDAEPFRST
                     YADAAWLLSQLEYLRNNFEASLEWAHVAADHGLIVRKWHTDFLESLRNVDVYRFRGAV
                     SDRIPIHIGKPEVPRVEVMVNHSKNVLAIIDSGAVMSIMSQGFASSLPVRKLGKFEGV
                     FSGLIGEPIPVQFGIIDSLQIGKITIENVPVAIMPDDKMSFVISGKHQFAIDFLLGAH
                     LLKELRIELDFRHNQVAFTHLLPADRVPANDQNLFWDQSRPAVRGVINRKGWFTFILD
                     TGSEVTFLNEAQVNSAQFQLFTKVHNATLQGLGGAEKHGEKVEDVEIGLDRWAGMFRT
                     LPMYEPGASEHERASGIIGENYLKNFVVTIDFGRMRLDLVPLLRMPVAEIEEQERPTG
                     GIPR"
     gene            15783..16691
                     /locus_tag="VNN25_19625"
     CDS             15783..16691
                     /locus_tag="VNN25_19625"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXH93799.1"
                     /translation="MEKGYQLHRGESLGESLCRICAWQTERIAKIVHDETMSMGERVH
                     EARKRIKESRALLRLFRGALGDAFDEENRRLRDAGRQLASYRDATTVVEAIKTLPPRV
                     RKAIGRKAMRTLRTFANERQHAQFAAESALRDRFESLIAGLVLVNERLAQASLPDTTR
                     FSIIEDGLARTISTGRRAMASAFKSGTDSDFHEWRKRVKDLGHHIELLINIWPNVMTA
                     LEKDISTLARHLGHHHDLALVAEIVEQTEGPLGDDAEMRRIGRLIAAQQKRTIALARP
                     IAERVYAIRSRRMARAIGTLWDVWQA"
     gene            16770..17312
                     /gene="hslV"
                     /locus_tag="VNN25_19630"
     CDS             16770..17312
                     /gene="hslV"
                     /locus_tag="VNN25_19630"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXH93800.1"
                     /translation="MEERPIIRSTTVLCVRRNGAVAMAGDGQVTVGTTVMKHGAAKVR
                     RLYHDKILAGFAGSAADAFALFSRFESKLEEYRGNMERSVVELAKDWRMDKYLRQLQA
                     FLIVANAERAYLISGTGDLIQPDDGLLAIGSGGPYALAAARALMQHTELSAATIAEDA
                     LRIAATICIYTNDQITVETL"
     gene            17437..18834
                     /gene="hslU"
                     /locus_tag="VNN25_19635"
     CDS             17437..18834
                     /gene="hslU"
                     /locus_tag="VNN25_19635"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXH93801.1"
                     /translation="MVIQLPGSSVDESSHSERLTPKEIVRELDTYIVGQHAAKRAVAI
                     ALRNRWRRQQLPADMRDEIAPKNIIMIGPTGVGKTEIARRLARLTKSPFLKIEASKFT
                     EVGYVGRDVESIVRDLTEIAVKLVKDEKTRLGRESAERNARERILDLLLPDSRRATAR
                     PAFVGAPDEPAPTETRDKLGVWLDEGRLDEREVEIDVKEQSFPSFEIFSAQGVEEMGV
                     NVKDMLPGLFGGKSKRKKMRLGDARPIIVQEEAEKLVDQQNVAREAIDRVEQSGMVFL
                     DEIDKIAGRQSAGGGPDVSREGVQRDLLPIVEGTTVNTKYGMVKTDHILFIAAGAFHV
                     SKPSDLIPELQGRFPIRVELESLTEADFVRILNEPKNALTTQYSALLATEGVTLTFAA
                     DAVAEVARYAALVNEKTENIGARRLHTILERLLDELSFDASEMNGQSVTIDAAYVQRV
                     LAETVKDEDLSRYVL"
     gene            18961..20010
                     /locus_tag="VNN25_19640"
     CDS             18961..20010
                     /locus_tag="VNN25_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXH93802.1"
                     /translation="MGTKRTARSVTVFEASATQVRNYRSAKREASARYLSASGGFSAL
                     AAMNPAASPKPDQNVVGVGIGEKISNGISTGVLAVKLLVRVKYPDDAIPAADRLPATI
                     GGLPVDVEQAGTFRRFASTATPAMPNPRGRFRPARPGSSIGFEDPQHSFTMAGTFGAV
                     VKRAGKLFVLSNNHVLADENRLPIGAPIFQPGLLDGGKVPQDRIAALAKFVALTTAGP
                     NKVDCAIAEAASANVLSKDILFIGPPNGTADAAIDMQVHKFGRTTGYRAGRITSIETD
                     ITVGYDMGNVLFTDQILIVGHNAQPFSAAGDSGSLILERGTNRAVGLLFAGSTSHTIA
                     NHLGEVLQALGVTLA"
     gene            20033..20266
                     /locus_tag="VNN25_19645"
     CDS             20033..20266
                     /locus_tag="VNN25_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93803.1"
                     /translation="MATHDEIRRLKDRYGQGLLGKRGVAGVGIEKDDGDNYALVVHLA
                     DASAKDDLPDELEGEKLRFVTSGPFVKLPALKH"
     gene            20449..21195
                     /locus_tag="VNN25_19650"
     CDS             20449..21195
                     /locus_tag="VNN25_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93804.1"
                     /translation="MRRLAATTVFLLAVPLYADSLADMRAAVSQLRATKPIHASIDVQ
                     RSRKNEGRFANSQFTGSASLDALEDESGLHITFPRAILERAERESRERASDPRKTTPT
                     RNAIDEMQTTDVADAVDFAHPFLRLIETAVKASESRGLREGRAVRVIVLKLTRKLPPE
                     ATSIFSVKFTEDQMTVWLGDDNIPVAAERIQRGSAGFMFIKGSMMNRSSWNFARYADR
                     LVVLRSDISYAGSGFGQKGEGRSVQVVTVR"
     gene            complement(21653..22081)
                     /locus_tag="VNN25_19655"
     CDS             complement(21653..22081)
                     /locus_tag="VNN25_19655"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXH93805.1"
                     /translation="MLPFTLLLEQIALLSLRALTGLVFVWSGFGHVRDPEGRSKDLGL
                     PKPIVLLVGVVELLAGLGVAGGLLTRLSAVGLMVIMLGAMHRKAFVWHSGFWGKNSMG
                     WHYDLIFFLIALMIFATNGGEWTIDRMLFGAKTVIGSAVL"
     gene            complement(22091..23194)
                     /locus_tag="VNN25_19660"
     CDS             complement(22091..23194)
                     /locus_tag="VNN25_19660"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH93806.1"
                     /translation="MNGFGAMFRKELTQMLRDRGTLFFAIAVPVFELILFGVIDMNAK
                     NIPTAIFDQSRSQESRRLIEQFGNTSYLHVTAMVASRAELQHQIVAGHAQVAVEIPPD
                     YARNLASHRQANVLIMIDGSDSSIANQALSAANGVVLFNGIQDLIEKSHAMPPVEAHP
                     VMMFNPDMRSANLLIPGLISILLTFSGTILSAFAIVKERERGTLEQLMVTPVSPMAVV
                     TGKLLPYLGLAYLQLLMILFLMRVLFRVPIHGSLTLLLILSPIYLLALLSAGLLVASR
                     ANSQMEAMQRAMGIMLPSVLLSGYIFPIASLPLPLRVVSYLLPATHYIRIARGIVIRG
                     ATFFDLWEPVVALLAISGVLIAASARAFQKRVT"
BASE COUNT         4608 a         7189 c         7057 g         4491 t
ORIGIN      
        1 cggcgagctc gattcccatc gcccgcgccg ttcgcgcctc ggtttcggct ggcgcagaga
       61 agaacgccac ggcgatcgaa acgatgacga gcaccgccgc catcaccatg ctctgccaga
      121 gaaagatcgt ctgcgtcagc ggaatcacgc cggtgatcgg cagcagcgcc ggcgggatcg
      181 atgacttggt ggcctgcagc aacgcagcgg acgacgacaa accgagcgcc caaacgctac
      241 cgaggccgag gtaggcggcg gcaccgatgg cgcgatagtc ggcgtcgacg cgcttcgtga
      301 tctcgcgcac cagcagcccg gcgaagacca gcgagaggcc ccacgacaag agcgaggaga
      361 gcatcgcgaa cgcggccaca aacgccaccg cgccccgcgc cgtcttcgga atcgcagcca
      421 gcctgcgcgt gagtgccgcc accggcggcg acgaggcaac gacgaatccg ccgacgatca
      481 cgatcgccat ctgcatcgtg aagttgacca gcgaccagaa tccgttccca aactccgtcg
      541 ccagcgtgac cggcttcacg ccccagccga gtcccgcgaa gaacacgagc acgatcgctg
      601 ccagcgcgaa gacgaaggcg tctggaaacc atcgctcgca aacgtcagcg atgcgcgagc
      661 cggcgcgggc aaggatgggt ggaggcatgg ggccattgta agcgcccgcc aagcgcagaa
      721 agccgaatag cacggggcca acgtacagca accatgtgga cccgccatct gcggtcgctc
      781 gttacgcggc gtcgcgggct cacacagtcc gctgaacgtt ccctctcacg agcaaaggac
      841 tagatgactt catccgagcc aacgttattg agttctcggt gatcgctctg gcgccaagcc
      901 agatcgcacc atacaagtta tagcttccgg ctaaaacatc gccgggaaca gttaaatacc
      961 gcgattgatt cgtcagtccc ggctctagag tgatctcttt gtcttggctc tgatcgaaat
     1021 actcagttcc gtcggcggca aggatacttg cgcccagcca gacttgatag ggaaattctg
     1081 ctttgctgaa aacgtcgtaa gaaatcctga ttgtctgacc tggcgacgcc tcctgagaat
     1141 ttgatgtaag acctctgatc tctactgctc ccaagagttg ccgaattcgg gccgcaacgg
     1201 cctcatccaa catcgcatgg cgttgcttgc tcgctcgcca cttgccagac acgtaccctg
     1261 cttcgatgat ggctattagg ccaacgatta gccccacggt ttcggtaggt gtcatctctc
     1321 ccgttccagt cttgagtcgc ctaatgtatc caagctagcg cggcgcggag ttcgacgaca
     1381 aggtcagcag cagacactag gcattcggct gattcgccga gcgtggctta cggaccgtcc
     1441 catgcagcag cgggccgcgc ggcgcgaact tgcaacccgg ttgcgatgcg gcaaaatagc
     1501 cagccgcgcc gtgtagacga acgttaaggg agcgagcatt agacgggctc ggaaatgaac
     1561 tcgatgaact cacgaccgag tccacttgtg cgttgctcgg cgagaccctg tgctgacatg
     1621 gtgctgtgaa gagactcggt attcaccaag cctcggctat aaaggtcgcg ccacacctgg
     1681 tcgtagaagc cgcgattgcc cgctattttg ggatatgcgt tttctaggat gtaagccatc
     1741 ccccccatca tgagattcgg ccagcgcgcg ccgaccagca cggtgttctt ctctggagca
     1801 tcgaataatt tgagaatgcg aatatgccag actgtgaaaa catcaataaa ggacaagaac
     1861 atttgctgaa gtgactgggc cggtgcagtc ggtaatgccg cgttgatcgc ggcgttccgg
     1921 agcgccgccc gcttctcgtc ttggcttgtt ctgagagcga tttgggtcgc ctgcaagacc
     1981 gtatctccga agacctcgtt gttcttaagt tcttcgattg gaatgccgcg tttgtcttcc
     2041 aaagtgcgta gggcttcggc gacttgctcc atccattcct gtcgccgccg ctcgagcatt
     2101 ggcgtgaaga cagcattgac gagttcggct gctggaccgc ccagtagggg cacggcggaa
     2161 actgccgcct ttgtgatggc ttgagctgca tcgccgactc caggtttcgg agggtcatac
     2221 tttttcgaca tggatctccg ctttgtcggc gtccggcgct gattggcgac gccgcggttt
     2281 tagtcttggg caggatcgcg cacccaccta ccaaatcacc ctcaccccca actgcatctg
     2341 ccgcggctca cccacaccac tctgcaccgt tccgtcgaag ttgaagatca ggttcgttac
     2401 ctcggggcga tggaaattct tgctgttcag gagattgaag atgtcgatgg ccggctcgac
     2461 cttgtaggcg ccgaagggga agtctttcgt gaagcggagg tccagcgagg aatagacgtt
     2521 gtccttgcgg ccgaggttgc gctgggttac ggagccgtcg ggattgatgc ggtcctgcgg
     2581 ggtctgcgcc tctgccatga accgcgacgc gccgaatgcg tctttgccgc agaaggcgac
     2641 gctcacgaca caggagagcg acttcggctg cgcggagcgg tacgaatagc gcacgttcac
     2701 atcgacgccc atcggcgcgt tccacaacag ccagctattc aggcggtggc gctgatcgcg
     2761 gtcggagtag ccgtattcag cggccagatc ggtgactttg gcgtagcgga aggtgaaggg
     2821 gtcgcgctcg ttgtcgtcgt ccgatttgtc tttcgagtag gtgtagttca cttcgaactg
     2881 aaggttgttc gatggacgct tgttgatgcc cagcgtaacg ccgttgtaga ggctcttggc
     2941 gctcgactcg atcgtcgtca gcgcgccgat tccgttcgca ccgccggcgc cgaggccggt
     3001 cgaccagggc gagccgagga ggggatcgtt gcggttgatg aagcgcgtga tgtgatcgcc
     3061 cttgatgtag ttgtacttga cgaggaatcc gtagtctttg accacttcct gctcccacga
     3121 aacgctggcc tgctgcgtgc gcgggttctg gaagtgtttg tcgaacacga agacatcggg
     3181 atcgaacggc gaaccgacct gcgattgcgg aatgatgttt gggtaggccg gtacaggccc
     3241 gaggattccg gtcaatgcgc tgttgcggaa gatcgtctgt ccacgcgagc cgttcgtcga
     3301 gcgggaagac gccaggtcga gccctggcac gcggccgtac tgaatgccgg cgctggcacg
     3361 cagaacttcc ttgccatcac cgcgcggatc ccaggagagt ccgaggcgtg gctgccacat
     3421 ttttctgtcg ctggggattt tgccgtttga cggaaactcc tgccctttcg atgtctttcc
     3481 gatgaagccg gcgtagaaca cctgatcggg aggcgtaatg ggatcgggct cgatctcagc
     3541 ctcccagcgc aaaccgtaat tgacggtgag gtgcggcgtc gcctgccagg aatcctgcac
     3601 gaaaacggca ggctcggttt gcgggatgct ttgcgttccc gcttgctcca cgctgatatt
     3661 gccgacgcct gcctgttgca gatagaggag cacgggaccg gtgatgttga atccagcggg
     3721 acacactccg gtctgggacg atgcaaagtt gttctgcgcg tccgcgcact cgacgtagtt
     3781 cggattcttc agatagttca ggaagccgtc ggtggagctg aagatgtagc ggccgttggc
     3841 aaatccgagg aaggtctgca cggaattcac gcgattgaac tcgaggcccg ccttgatgtc
     3901 gtgcgcgccg cgcaggaaat tgacgttgtc gttgaactgc acgcgcgtgt cgtaatactt
     3961 gaccggaatg aagaacggct cgccgaagcg gtagcttttc gcaaagtcga atgcggtgtc
     4021 gggcagtggg cggttctgtc ccgtaatatt cggaccgttg taagggcgcg gccggttttc
     4081 ttttgcggcc tggaaccgga actcgttgaa ggtcgacgat tgcaatgtcg acgtcaatcc
     4141 gccgacgacg gattccgacg agtctttctc gatcgcgttc gcactgcgtc cccacgagtc
     4201 gacgtcgaac gttccgttgg cctgctgcga ccatgtgtag ttgtagcgaa ggttggcgag
     4261 gttcttcgcc gaggcgttcc aatcgagttt gagaaggagc acgcgcgcgt cgttcgttcg
     4321 cgcaatcgat ccgttctcgt tcggactgcc gagcgaggcg agggcgtcga caacccgctg
     4381 ttcgatgcgc ccgggattcg tctgcttggt cgtctttccc ttctgatagt cgagcgcagc
     4441 gaagtagaag agtttgtcgc gctgcagcgg cccgccgagg gtgaagccgg cctgtgtctg
     4501 cgaagaaggg aacttcggcg aggtcgtgcc gtcgggattc ttgggctggc tgctcagtga
     4561 gtcgttcttg aacgtcacgt gtgccgtgcc ggcggagtcg ttcgttcccg acttggtgat
     4621 gacgttgacg aagcccgaca tggagcgccc gaactccgcg cccgcaccgt cggagatgac
     4681 gaccatctcc ttgaccgcat cgagattgaa tgtgaacggc ggccgctgcc cgccgcgctg
     4741 ctcgccgaag aacggattgt tgaagtccgc gccgtcgacg gagacgttgt tcgagatgcc
     4801 tttctggccg ttgatcgaca gctcgtcgcc gtcgggtccc tggacgatgg tcacgccggg
     4861 cgtgagcttg gtgaactcga ggaagttgcg gccgttgttg ggcagcccct ccatcgcctt
     4921 ctcatcgacg cgcgtcgccc cttccgttcg cgccgtctcg atcagcgggg gcgccgcggt
     4981 aaccacgatc tcctcggcgg tcgccgcctg tttcagcacg accggcaccg tcagcgtctg
     5041 gccgacgccg agcgcgagac ctttttgcac gacggtggcg aagccttgca gcgccgcgct
     5101 cagttgatac ggtccgaccg gtagaagcac ggcgcggaaa cggccgccag agtcggtgac
     5161 cgaaatgctc tcgtagccgg tgcccgtgtt cttgacggtc acggtgacgc cgggaacagc
     5221 gcctccgctg gcatcgacaa ccgtgccttc aatgacaccg gtcgttgcct gagactgcgc
     5281 gagaagggga agcgctacga cgaggacgag aagcgccagg agcacgaagg acggacgacg
     5341 catacgggtc tccttttttc tttcgagcgg caattggtgt gtgacgtcag gatagttcgg
     5401 aactcacgac tgacgcaatg gcgggatctg gaaagtgatg atgattgcgt tggtcaccgg
     5461 ctcacatacg gatttccacg aatgaaaaaa cggagcggcc agcgtgctgc ttcaccggag
     5521 ttttcgatgc cgatgcggtg gctgacggcg atgtcatcct cgtcgacgac gtgatcgcgg
     5581 cccgtgagcc agagcgcgtt tccatcgagg ggctcgccgt cgagcgactt gccgacgccc
     5641 atcgccgagc agagtttggc gggtccgctc atcagatcgc gatcgcgttt cgctttcgga
     5701 cggcgctcgc gcatcgcctc gatccctcgc aacggttctg cggcgcgcaa cagcacggcc
     5761 tcggcgatat ctccttctgc ggtgaccacg ttcaagcacc agtgcattcc gtacgtgaag
     5821 tagacgtagg cgtgaccacc ttcgagatac atcgactcgt tgcgtgcgct gcgcagtccg
     5881 cggcgggcgt ggctggcgga gtcgttggcg ccgaggtagg cctcgacctc gacgagccgc
     5941 gcggccagca acgcgcgccc gatgcggcgc cacaacacgc agccgaggag agagcgcgcc
     6001 acggtcaccg tgtcctggag atagaacgac cgagggagct tcgcggtcat gccgaaggaa
     6061 gcgcccgatt cgacgagcgg cgatcgagtt gatcttcgct gacgaatcga ttgatgagac
     6121 gaacgaggtc cgcgtggact cccggtggcg cgccgatgat gttgccgcgc tcgagccagc
     6181 ggttgcctcc cgagaaatcg ctgaacacgc ctcctgcctc ggtcacgagc aacgaaccag
     6241 cggcaacatc ccacggtgat aggtggagct cgaagaagcc gtcgaagatg ccggcagcgg
     6301 tgtaggcgag atcgatcgcg gctgagccgg cgcgccgcac acccttcgag acactgatca
     6361 cttcgcgaaa gatttcggtg tagacgctga cgaattgctg ggcgcgaaag ggaaagcctg
     6421 tcgcaaggaa cgctccttca accgtcgcct gactagagac gtgcatccgc tcgccgttgc
     6481 gaaacgcgcc ggcgccacgc tcggcagtga agaagagatt gcgcagcggc tcgtgaacga
     6541 cgccggccgt gatctcgtcg ccggtcttga gaccgatcga gaccgaccag accgggaagt
     6601 gctggaggta gttcgaggtg ccgtcgagcg gatcgatgat ccatgttcgc ccgctggtgc
     6661 cctcggcgct gatgccgcct tcttcgccga ggatggcgtc gtgcgggaaa tgttcgcgga
     6721 taaacgccac gatcgctgct tcactctcgc ggtcggcgcg tgagacccaa tcgttgcgcg
     6781 acttcaggtc ggcatcgtcc ttcccgaggt gttcccaata tttcagaagg attgccgcgc
     6841 cgagcgtggc tgcctgcgcc gccaactcgg cgtcattact atgggtcgtc atcgagcgat
     6901 tctatccgcg ccgatgagct ctgccgcggc cacgtttttt gtccgtctcc ccgtcaccga
     6961 ctagcccctc cctcggatgt tatgctccaa aagggtcgat gcaaccgcca cggatttcac
     7021 gatatcgcgt aggacgtctc atcggcgccg gtggaatggg cgaggtatac ctcgccgagg
     7081 acgagacgct caaccgcaag gtcgcgctga agttacttcc ggtgcgcttc acgcaggatg
     7141 aagagcgcgt tcgccgtttt cagcgcgaag cccgggccgc ctcggcgctc aatcatccga
     7201 atatcctcac gatctacgag atcggccaca ccgactcgct gcactacatc gccaccgagt
     7261 acatcgaagg cgagacattg cgcgaccgaa tggcgaggca gcggctcaag atggccgagg
     7321 tgctcgatgt ggcaaccggc gtggccagcg cgcttgcggc ggctcacgac gccggcatca
     7381 tccaccgcga catcaagccg gaaaacatca tggtccggcc ggacggcgtc gtgaaagtac
     7441 tcgacttcgg cctggccaag ctggcagatc cggagtccgc gctcaaagag agctccagcg
     7501 gcggaatcat gggaacgctg ctctacattt cgccggagca ggccagcggc cttcaaccgg
     7561 atgcgcgctc agacctctac gcgctcggcg ccgtgatgta cgagatgatc accggccacg
     7621 ctccgctgcg cggcgagagc ttcatcgaca tcgccttcgc catcgccaaa gagaaaccga
     7681 agccgccgtc aagctccgtc gatggcgtgc cgccggagct cgacagcatc attctgaagc
     7741 tgctggaaaa agaccgcgac cgccgctacg cctcggcgcg tcaactcctc ggcgatctca
     7801 atacgcttcg gagagagctg gagttcgaga acaaactcaa ctcgctggag atgcagcgcc
     7861 acgtctccgc gcccggattc gcgcagcaac cgacggcgca gatgacgttt ccgatcaaca
     7921 cgcgagccag ggttcggcgc gcgtttcgcg accggcgcgt cgtcgtgccg ctcgccttcc
     7981 tcaccgtcgc gctgctggct ggcgcagcgt acatgatcat gaagaggggg atattcgccg
     8041 accggatcga cagcgtggcc gtgcttccct tcgtgaacgt atcgggcgat ccgaacaccg
     8101 aatatctgag cgatggactg accgacagca tcatggattc tctttcgcag ttgccggaga
     8161 tccaggtcgt cgcccgatac aagggaaaag gcagtgatcc gcttgcagtc ggacgagaac
     8221 tgcacgtgcg cggtgtcatc acaggccgcc tgattcagcg tggcaacacg atcgtcatcc
     8281 gcgcctcgct gaccgacgtg acgcgcggca cgcaggtgtg gggccagcag tacgaccgca
     8341 gtctttccga cgtgatcgcc ctgcagaaag atctatccga ggagatctcg tcgcagcttc
     8401 aaccgcacct gaccggtgag gagcggaagc tgatgagtcg cagccggccg gagagcaacg
     8461 aggccttcca gctctacctg aagggtcact actacgcgac caagttcaac gacgacgagt
     8521 cgatccgaaa gggaattgct tatttcaatc aatcgatcga gcgcgacccg acctacgcgc
     8581 tcgcttacgc cgggctgtcc gcggcctact atcagctctc caacctcttc atgcctccgc
     8641 gggaagcgat gccacgcgtg cgcgaagccg ccgagcacgc tctcgcgctc gaccagtcgc
     8701 tcgcgcaggc tcacatgtgc atggcgctca ccctcacctg gtatgactgg gattttgtaa
     8761 atggcgagaa ggagtttcgc cgcgccattg cgctgaacgc caatgactcc gaggcacaca
     8821 aggcgtacgg tgatcttctg ggggcgatgg gccggttcga cgaagcgatc gtagagaaac
     8881 gccgcgccga gcagctcgat cccctgggcg tttcgacaag ttgggacgcc ggccgcatct
     8941 tgttctacgc cggccgcgat gaggaggcca tcgagcaggc caaacgagcg ctcgatctcg
     9001 attcgaaatt tccctacatc tacgacctcg aagcacagat cgcttaccgc aagggcaacc
     9061 ttccggaggc tttgtcgctt atgcagcaag cgttgcagtt cggcggacac aagcaacttc
     9121 tcatcaccga ctggggaatg ctcaacgcgc gcgccggcag acgcgacgta gcgctgggcg
     9181 ccatgaacga gctgcgagac cgagccacct acacgctccc tctttttctg gcccgcatct
     9241 acgccgccct cggcaacaac gacgaagcga tgaagaacct ggagaaggtc tacaacgacc
     9301 gctcggagtc tgccgtctgg ctgaaggtgg atccgtcgct ggagacgctg cgaaaggatc
     9361 caaggttcat cgcgttgatt cggaaggttg ggttgtagga gtgattcttt ctcgactcac
     9421 cgcgctcgtt gcatggtttc ccgccactgc tcttctaact ccagagcagc gatccatctc
     9481 tcgcaagggc agtacggaac ttttcgaaaa cgggcttgac gctcaacggc gtttgccaaa
     9541 cccatagacg agttgaagaa acgactcgcg gatatcgtct tccgataatt ccggatggcg
     9601 ccggcggatc cctgcaaggg tcgtttccct gacggcatcg ctcagatccg cggcaatctg
     9661 cgcgcgccgg gcgggcccga cagcccggta gagctccaaa tggatcgcgg ccaccttagg
     9721 ggaagtgtcg gtgacagaca tcgaagaaac gataccacgg gcctcaggcg cgttgaatgt
     9781 tcgcctaaca acgcctggct ttctcccctc ggggtctgac cctttcgcaa ctggtgcgtc
     9841 acttccactt tcgggtccga ccccttccgc gactggaagc cgctctgctc gacatgggag
     9901 acactccctt tcacatcaga aacgctccgt ttgatatcgc aaacggtccg tttgacgttg
     9961 gagatgctcc gttcgaaaac ggagatgttc cgtttgatat cggcgacgct ccgtttctaa
    10021 atggacgtgc tccgtttgca attggagacg cgttccgttt cgaattggag aaggtccgtt
    10081 tcgaaacggc gagagtccgt ttcgaaacgg aggtgttccg tttgacattt ggaaatctcc
    10141 aatgacgagc gccgcgtcgc ccacctcgag acacccgttt ccaatactaa agcaccgaaa
    10201 atggcaacat cgacaaattt tgacacttca atccaacacc ccacgccccg agtagcgtca
    10261 ctttcacttt gggtctgacc cctcacaccc ccactccacc ccccgggtag ggaaatcccc
    10321 cgtttgatcc tcacctatgt acatgtccat gcgtggcacg gcacggcgcg cgcggtctgc
    10381 aatcaccatc gatcttcggg agttcgggtg ggaccgcgcg ctgatcgcaa cgatgcgtca
    10441 ttcgacgctg ccgtggttcg acggctgtgt gccggtcgtt tcgctcgatg ccacgcctct
    10501 tgccgaggtg gcggagtttg gcccgcgcga caggctgtct ctcgtcgcgc aattctcggc
    10561 gcatcaggcg ctccttcact tcgccggatt ttcggacgcc gagctcgatc cggcggagtg
    10621 ggcggtcgtg cgaaagcgtg gcaatgactg ccggcttgtc cgcaccgccg cgcgcacaac
    10681 ggcggacgaa gcgcccgcgc tcacggtaat ccagcagttc gccgaagtgg tttccgcgcc
    10741 gccgctggac tcgttgcggc agtcgtgggg acgcgccgag tccgtctacc tcgaggcgga
    10801 agcgcggctt cgcggcgacg cagcggctga ccttcagtgg atgcgccgtt cggcgttcgg
    10861 agcgatcagc gcacccggcg ccgacgcgct gcgggaaatg cttcggagcg gtgtgggccg
    10921 gtttcgcgca tccgatgcca tcgacagcat ccgcgtggcc gccgcactcg ggagcgaaca
    10981 gatcattgcg attggtcccg gcgcctcgcc tcttcagcgt tattcggcgc ttcgcgcgct
    11041 tgcgccaatc acgggcgcta tcgagcgctg cggagagagt cagatcgtcg agcgcgtggt
    11101 tgaagccgcc tcgcggcaac acctcatctt ctcggtcacg aatcgcgatt cgttcgacgc
    11161 ctcctcggca cgcgtcatcg agatgctcgc tgccacggac gccggcatct ggattatcga
    11221 cgacggcaac gaatcggacc tccctccttc tcgcttcttc gtcgtggctc ctttcctggc
    11281 cgcgcgccgc gacatcgagg agcgattcgc tgcgttcacc gcgccggacg cacggtcgtg
    11341 gctcgagcac ttcgtggagt ccgcagcctt tgcccgtttt ctcgacaccg gcacgctgcc
    11401 cgaggaggag cgtgcgacgt cggtgagctc ccttcgtgaa ccggcccgct cctgcatcgc
    11461 cgcgctcgca cttctcggca cacgggttcg tatcgacata gcatcggcgt tttttcagca
    11521 gctcatgttc ggcggtgtcg caggagaact ggctatcgaa ggaatcacct cgatcgagga
    11581 cggccacatc gtctttgcat ccgacgctat ccgggttgcc gcgaaaaacc tgattccttc
    11641 gtcgtcgcgg ccgatgcttt cccgcgtcgc tgccggcgtg atggccggaa ccgcgggcga
    11701 cgtttccggc gccgccgcgc tcttgatcga cgccggcgat ccgctcgctg ccgcggaact
    11761 tctcgaacaa gtgacggtca caggtgacca acatctcctg ctgcttcggt ccctgcctcg
    11821 ccaggcgttg acaccaaaac tggcccggac gctcgcctcg gcgttgatcg agcggggacg
    11881 ctaccgcgac gcccgcgaca tcgtgctgct gcttaccgaa gaggtgcgcg agctgttcct
    11941 ggcacgcatc gagcgccgcc tcggtgacta cagcaccgca cgatcccgtc tcgagcgagt
    12001 taccaaacga acgtttgatc acgatgcgct gcgcatcgag ctccttcacc tggagagccg
    12061 ctacgacgac atgcgtgatg cgctggcggc atgcaccgct ccgcaaggcg aggaggagcg
    12121 tgcgcggctg gcctatctcc gcgcactcca tgcgcacgag acaggcgcgc aaaccgatcc
    12181 gaaggacgat gcggaacttc cgcccgatca ctatttcgct tcgcgcctgg ccacctatcg
    12241 cgccacttca cgtcaggatt ttgacgacgc ggccggccac gccgcgaagt cgttcgcgcg
    12301 cgctcgtgac gccgctgagc gaatcgatgc tgcgctcgac cgcgtctaca ttctctttac
    12361 cgccggagca tggcaggagg cgcgcgagga agccttgcgg actttggctc tagtcgaaga
    12421 gacacaaggg gacccggccg ctggcggcat tcttttcatc ctggcgtacc ttgctgccga
    12481 cgatggccag tgggctcacg ccgcgcagca catccatcga ctgagaaact tctttcgagt
    12541 gacgagtgat gagaggcggc tcctcgagac cgagcttctc tcggcacacc tcgatttctc
    12601 gcgtgggcgt ttcagtgacg ctatgcgctc cgcccagttc atcgccgatt cgaattttac
    12661 ggcgcaaatg cgcgaagccg ctgcgctgat cctcgatgag atcgactgga tggagagccg
    12721 cgaaacgccg ctgcgctcga ccggaacatc gggaaatgtc gagctgacga gacgcaacga
    12781 gatgctgcgc atccgccgct ccggcggtca cgttgatctg agcggcgcaa taaaccgttc
    12841 acaacgactg caactcctcc gcgatgcgat cggctgcggc cggagcgagg aagccgcgag
    12901 catcgcagca gaaatgggag tcgaactccc cgtcggtgaa gggaacgctt cgtcgctgga
    12961 gctgaggatg ctgcgcgacg ccgccctgcg cgagtttcca ttcgggccgc gcgactttgt
    13021 cccgacaccc tggcggtttg ccacacgtaa ccgcctcggc caatggcacg agatcggatc
    13081 actctctccc gctcacgcca cggagctcga tcgtgttctg gcgtcggcgg cgaccgactg
    13141 gatcatctgc agcgatcgcg agcttctctt cgtcgacggc atcagccgct ggtgcgcgga
    13201 gtcgcgcgaa gcgttggcgg caatcttccg cacgcgcgcc gggcatcacc ggatgcagcg
    13261 cgtcatcgaa cagaacgaaa acgtcaccgt cgccaagagt gaggccatcg acggaatcat
    13321 cggcgactcg ccggccatgc gtgagctggg ctcgctcatc atccgtgtcg cccgccgcga
    13381 cgtacccgcc tgcatcctgg gcgaatcagg caccggcaag gagctggtcg cgcgcgccat
    13441 ccatcgttac tccccgcgcc gcggcaaaac gttcaccgcc gtcaattgcg cggcattgcc
    13501 tgagaatctg atcgagagcg agctctttgg ctgcgttcgc ggcgcgttca ccggagccga
    13561 tcgcgaccgc gccggtctga tcgagacgag tgaaggtgga acgctttttc tcgacgagat
    13621 cggtgagatg ccactcgttg cgcaagcgaa actccttcgc ttcttgcagg agggtgaatt
    13681 ccggcgcgtt ggcgacactg tgaaccgcac ggcggacgtc cgcatcgtca gtgccaccaa
    13741 tcgcaagctc gaagccgcgg tcgaagaggg ccggttccgc gaagacctct attaccgcat
    13801 tggaggcgtc gagatcgtgc ttccaccgct gcgcgaccgc gtcaccgatg ttgcgatgct
    13861 ggccgcacac ttccttgcga aggagcagga gaagagccgc ggcggaccag ccaggctgac
    13921 ggccgacgcg gagtccgtct ttctcgccta tcgctggccc gggaacgtgc gcgagttgca
    13981 gaacacgatt cgcgccgcac atgcattggc cggcgatgcc gcggccatcg atgtcgagca
    14041 tctgcccgaa cggatgcgca gcgccgtcgt gcgcggaggc gccggcatca gctcgtatca
    14101 ggatgccgtg acccgtttcc gccgcgacct gatcgagaaa tcgctcctgc aagcgaacgg
    14161 gaaccagaat cgcgccgctg cgtccctgag catgtcgcgt caggctctgg cctaccagat
    14221 ccgcgagctg ggcatcctcg tgcggcctca cgttgtcaca gagcgctgac ccggcggacc
    14281 cggcatcgtg tcacatcagt ccgtacaatg agcacaccgc catgcgccat ttgcccgcaa
    14341 tcgccgctgt cgcggtcgtc gccggatgtg ctctctactc cgatgtgtcg atctcgccgt
    14401 tatcgttagc acccggcgcg atcgatcagg gtagcgatct gccttccatg ctacggaaga
    14461 gcgattacct tcgcgcagtc gaacaggctt cactggtcga tgccaagacg aagcgaaacc
    14521 cggccgagct cgtctccctc ggctcggcgg aattagcagc aggacagttc gacgttgccc
    14581 ggcgccacct ccgcgccgca ctcgatgccg agccgttccg ctcaacctac gccgacgccg
    14641 cgtggctcct ctcccaactc gagtaccttc gcaacaactt cgaggccagc ctcgagtggg
    14701 cacacgtcgc cgccgatcac ggactgatcg tgcgcaaatg gcacaccgat tttctcgaat
    14761 cactgcggaa cgtcgatgtc tatcgtttcc gaggggcggt gtcggatcgc attccgatcc
    14821 atatcggcaa gccggaagtg ccgcgcgtcg aggtcatggt caatcattcg aagaacgttc
    14881 tggcgatcat cgactccggt gccgtcatgt cgatcatgtc gcagggattc gcttcctcgc
    14941 ttcccgtacg caaactcggc aaattcgaag gagtcttctc cgggctcatc ggcgagccga
    15001 tcccggtcca gttcgggatc atcgattcgc tgcagatcgg gaaaatcacg atcgagaacg
    15061 tccccgttgc catcatgccc gacgacaaga tgagcttcgt catcagcggc aaacaccagt
    15121 tcgcgatcga ctttctcctc ggtgctcacc tgctgaagga gctgcggatc gagctcgatt
    15181 tccgccacaa ccaggtcgcc ttcacccatc tcctgcctgc cgaccgcgtt cccgcgaatg
    15241 atcagaatct cttctgggac cagtcccgtc cggcggtgcg cggcgtcatc aatcgcaaag
    15301 gttggttcac cttcatcctc gacaccggct cggaagtgac tttcctcaac gaagcgcagg
    15361 tcaactcggc tcaatttcaa ctcttcacca aggttcataa cgccacgctt caggggcttg
    15421 gcggcgcgga aaaacatggc gagaaagtcg aggatgtgga gattggcctt gaccgctggg
    15481 ccggcatgtt tcgcacgctt ccgatgtacg aaccgggcgc atccgagcat gagcgcgcct
    15541 cgggaatcat cggagagaac tatctgaaga acttcgtcgt cacgatcgac ttcggccgca
    15601 tgcgcctcga tctcgtccct ctcctgcgga tgccggtggc cgagatcgaa gagcaggagc
    15661 gtccgaccgg aggcatccct cgttgacgtt tcctacgtcc tggacagctc gaacaggcat
    15721 gcgccgctgc tgagctgccg cgagcgatct ctcagggcgc gaaatcctag aataacgtcc
    15781 gcatggaaaa agggtatcaa cttcatcgcg gggaatcgct cggcgaatcg ctctgccgaa
    15841 tctgcgcgtg gcagacagaa cgaattgcga agatcgttca tgacgaaacg atgtcgatgg
    15901 gggaacgcgt tcacgaggcc aggaagcgga tcaaagagtc acgtgcgctg ctgcggcttt
    15961 ttcgtggcgc gctcggcgat gcgttcgacg aggagaatcg ccgactccgc gacgccgggc
    16021 ggcagcttgc gtcctatcgc gacgccacga cggtcgtcga ggccatcaaa acgcttccgc
    16081 ctcgggtgcg caaagcaatc gggcgtaagg cgatgcgaac actccggacg ttcgcgaacg
    16141 aacgccaaca tgctcaattt gccgccgaat ccgctcttcg cgatcggttc gagtcgttga
    16201 tcgccggtct cgtcctcgta aacgagcggc tggcacaagc ttccttgccg gacacgacgc
    16261 gcttttcgat catcgaagat ggtcttgcga ggacgatttc caccggcaga cgcgcaatgg
    16321 cctcagcctt caaatccggc accgattcgg actttcacga gtggcgcaag cgtgtgaagg
    16381 atctcggtca tcacatcgag ttactgatca acatctggcc gaatgtgatg actgcgctgg
    16441 agaaggacat ctccacgctc gcgcgacacc tcggccatca tcacgatctc gcgctggtcg
    16501 cagagatagt tgaacagacc gaaggtccac tcggcgacga tgcggaaatg cgtcggatcg
    16561 gccgtctgat cgccgcccaa cagaagagaa ccattgcact cgcacgtccg attgctgagc
    16621 gcgtctacgc gatccggtcc cggcgcatgg cgcgcgctat cggtactttg tgggatgtct
    16681 ggcaagcttg aacaaacgtt tggccggctc aacagcgttg caggcgacgt tcatggaaat
    16741 cacacgctct gcgatacgat catgccggta tggaagaacg ccccatcatt cgctcgacga
    16801 ccgtgctttg cgttcgccgc aacggcgccg tggcaatggc cggcgacggg caggtcaccg
    16861 tcgggacaac cgtcatgaaa cacggcgccg ccaaagtgcg tcgcctctat cacgacaaga
    16921 tcctggccgg ttttgcagga tccgccgccg acgccttcgc gcttttctcc cgcttcgagt
    16981 ccaagctcga ggagtaccgc ggcaacatgg agcgctccgt cgtcgagctg gctaaagact
    17041 ggcggatgga caagtacctg cgccagttgc aggcattcct catcgtcgca aatgccgagc
    17101 gcgcctatct gatctccggc accggcgatc tgatccagcc ggacgacggc cttctggcca
    17161 tcggctcggg cggcccgtac gcgcttgccg cagcgcgggc attgatgcag cacacggaat
    17221 tgagcgcggc aacgatcgcc gaggacgcgc tgcgcatcgc cgccacgatc tgcatctaca
    17281 ccaacgacca gatcaccgtg gaaactcttt gaaagcagag actagagatc agagattaga
    17341 gatcaggaag cacaaatctg ccctgatctc ttgtctctaa tctcttgtct tctaatctct
    17401 gatcttctgt ctccaaatct ctgataaatc tcaatcatgg tcatccaact tcccggcagc
    17461 agcgttgacg aatcaagcca ctccgagcgg ctcacgccga aagaaatcgt gcgcgagctc
    17521 gacacctaca tcgtcggcca gcacgcagcc aaacgcgcgg tagcgatcgc gctgcggaac
    17581 cgctggcggc ggcaacaact ccccgccgac atgcgggacg agatcgcgcc gaagaacatc
    17641 atcatgatcg gaccgacggg agtcgggaag actgagatcg ctcgccgtct ggcgcggctc
    17701 accaagtcgc cctttctgaa gatcgaagcg tcgaaattca ctgaagtcgg ttacgtcggc
    17761 cgcgacgtcg aatcgatcgt ccgcgacctc accgagatcg ccgtcaagct cgtaaaggac
    17821 gaaaagaccc gcctcgggcg cgagagcgcc gagcgcaatg cgcgcgaacg aatcctcgac
    17881 cttctgctgc ccgattcgcg ccgcgccacc gcacgccctg cgtttgtcgg tgcgcctgat
    17941 gaaccggctc cgacggagac gcgcgacaag ctcggagtct ggctcgacga aggccggctc
    18001 gacgagcgcg aggtcgagat cgacgtcaag gagcagagct tcccgtcgtt cgagattttc
    18061 tccgcgcagg gcgtcgagga gatgggcgtc aacgtcaaag acatgctccc cggcctcttc
    18121 ggcggaaagt cgaagcgcaa gaagatgcgc ctcggcgacg cacgcccgat catcgtgcag
    18181 gaagaagcgg agaaactcgt cgatcagcag aacgtcgccc gcgaagcgat cgaccgcgtc
    18241 gaacagagcg gcatggtctt tctggacgag atcgacaaga tcgccggccg ccagagcgcg
    18301 ggcggcggcc ccgacgtctc gcgcgaaggc gtgcaacgcg atctgctgcc aatcgtcgaa
    18361 ggaacgacgg tcaacacaaa gtatggaatg gtgaagaccg accacatcct cttcatcgcc
    18421 gcgggcgcgt tccacgtttc gaagccgtcg gaccttatcc ccgaactgca gggccgcttt
    18481 ccaatccgcg tggagctgga atcgctgacc gaagccgatt tcgtgcgcat cctgaacgag
    18541 ccgaagaacg cgctgacgac gcagtactcc gcgcttttgg ccaccgaggg tgtcactcta
    18601 acgttcgctg ccgacgcagt cgccgaggtg gcgcgttacg cagcgctggt caacgagaag
    18661 acggaaaaca tcggcgcccg ccgcctgcac accatcctgg agcgcctgct cgacgagctg
    18721 tcattcgacg ccagcgagat gaacggccag agcgtgacga tcgacgccgc gtacgtacag
    18781 cgagtgctcg cggaaacggt gaaggacgag gatttgagcc ggtacgtttt gtaggaaccg
    18841 ccgttttggt ccgacggctc gccattcgtc atgaccgcaa gacctatttc aggattacgt
    18901 cagtaacagc gccgaggcgg gaaaacagta atatagtccg tcccatgagc ggaggttctt
    18961 atgggcacga aacgcaccgc tcgatcagta accgtctttg aagcttcggc aactcaggtt
    19021 cgaaactatc gatccgccaa gcgagaggcc tcggcacgct atctttcggc atcaggcggg
    19081 ttttctgctt tggcggcgat gaatcctgct gccagtccca aaccagatca gaacgtagta
    19141 ggcgtcggca ttggcgagaa gatatcgaac ggcatctcga cgggcgttct tgccgtcaag
    19201 ctacttgttc gcgtgaagta tcccgatgac gccatccctg ctgccgaccg tttgcctgca
    19261 acgatcggtg gtctgccggt tgatgtcgaa caggccggta ccttccggcg cttcgcctca
    19321 actgctacgc cggcgatgcc gaatccgcgc ggtcgttttc gcccggcacg tcctggctcc
    19381 tcgattggat tcgaagatcc tcagcactcg ttcacgatgg ccggcacttt cggcgcggtg
    19441 gtcaaacgag ccgggaagct attcgtgctc agcaacaacc acgtacttgc tgatgagaat
    19501 cgccttccga tcggcgctcc gatttttcaa cccggattac tcgatggcgg caaggtgccc
    19561 caggaccgca tcgccgcact ggccaagttc gtcgctctga caaccgccgg accgaacaag
    19621 gtcgattgcg cgattgcaga ggcagccagc gcaaacgtac tgtcaaagga cattctcttc
    19681 atcggcccgc caaatggaac ggctgatgca gccatcgaca tgcaggttca caagtttggc
    19741 cggacgaccg gctacagggc cggtcgaatt acgagcatcg agacagacat cacggtcggg
    19801 tacgatatgg gaaacgttct gttcaccgac cagattttga tcgttgggca taacgcccag
    19861 ccattcagtg ctgcgggcga ttcgggctca ctcatcctgg agcgaggcac caaccgcgcg
    19921 gtcgggttac tgtttgctgg gtcaacatca catacaattg ccaatcatct cggcgaggta
    19981 ttgcaagcgc tcggagtaac tctggcgtaa ggctcgcaac atcggggaga cagtggcaac
    20041 acacgacgaa atccggcgcc tcaaggatcg ctacggccag ggtcttctgg gcaaacgagg
    20101 tgtggcgggt gtcggaatcg agaaggatga cggggacaac tacgcgttgg tcgttcatct
    20161 cgctgacgca tcagcgaagg acgatcttcc tgatgagctc gagggtgaaa agctgcgctt
    20221 cgtgacttca ggcccgtttg taaagctgcc tgcgctcaaa cattgactgc ctggacgagc
    20281 gaacgctttg caatacagag acgcaaaggt cgcagagaag gtttcaactc tgtgtcctcc
    20341 gtgtctctgt ggtgcttgtt tcttctgagc tgcgagatcc gctcccgctc gaacactgcg
    20401 gtgaacgttt gcgtcatgta ggtgtggtta ccgcatactt tccggtgcat gcgacgcctt
    20461 gccgctacca ccgtcttcct cctcgccgtt ccgctctatg ccgactcgct cgctgacatg
    20521 cgcgcggctg tctcgcagct tcgggccacg aagccgatcc acgcctcgat cgacgtacag
    20581 cggtcgcgaa agaatgaggg gcgtttcgca aactcgcaat tcactggctc ggcgtcgctc
    20641 gacgcgctcg aggatgagtc agggctgcac atcacgtttc cgcgggcgat cctcgaacga
    20701 gccgagcgcg agtcgcgcga gcgcgcgtca gatccccgaa agacgacacc cacacggaat
    20761 gccattgacg agatgcagac caccgacgtg gccgatgccg tcgacttcgc gcatcccttc
    20821 cttcgtctga tcgagaccgc ggtgaaagcg agcgaatcaa ggggcctgcg cgagggccgg
    20881 gcggtgcggg tcatcgttct aaaactcaca cgaaagctgc cgcccgaagc gacgagcatt
    20941 ttcagcgtga agttcaccga agaccagatg accgtctggc tcggcgatga caacatcccc
    21001 gttgccgccg aacggatcca gcgcggctcc gccggattca tgttcatcaa aggctcgatg
    21061 atgaatcgtt cgtcctggaa cttcgcgcgc tacgccgacc gcctggtcgt cctccgcagc
    21121 gacatctctt acgcgggatc aggcttcggg cagaagggcg aaggacggag cgtgcaagtc
    21181 gtgacggtgc ggtgattgga gattaccgaa ccatcaacga tgacgactcc gaaatccggg
    21241 atgccgattt cacttagagg atggtcatcg atgatttcca ggatgccgat ttcgctcaaa
    21301 agatgtcatc gatgacttcc atgatgccga ttttggttaa taaatgtcgt cgatgacatg
    21361 caggatgcaa aatttgctta aaagatgtca tcgatgacta aaaatatgtc atcgatgcct
    21421 tccaagaagt aaattttgct caaaaaatgt catcgatgtc atccgggatg ccgttttcac
    21481 tcggaaaatg tcatcgatgt catccagtga tcgcgatttc gagtaaaaaa tgccgatgtg
    21541 tcatccaggg atgacgatct cgcacgaaca ctgacaacgt tcacattgaa aatgacggcg
    21601 tcgagacatt cctatctcat tcgtcacgcg gcatgttaac cgcccgtcgc tcttagagca
    21661 ccgcactccc gatcaccgtt ttcgcgccaa acagcatccg atcgatcgtc cattcgccgc
    21721 cgttcgttgc gaagatcatc agcgcgatca ggaagaagat caggtcgtag tgccatccca
    21781 tcgaattctt gccccaaaaa ccgctgtgcc agacgaatgc tttgcggtgc atggcgccga
    21841 gcatgatcac catcaggcca acggctgaga ggcgcgtcag gagtccgccg gcgactccga
    21901 gtcccgccag aagctcgacg acgccgacga gtaacacaat gggcttcgga aggccgaggt
    21961 ctttcgagcg cccttccgga tcacggacgt gaccgaaccc gctccaaaca aaaacgagtc
    22021 cggtcagcgc gcgcagggac aaaagtgcga tctgttcaag gagaagagtg aacggaagca
    22081 tgaacggaat ctacgtcacc ctcttctgaa atgcacgtgc gctcgcggcg atcagcaccc
    22141 cggaaatggc cagcagcgcg actaccggtt cccaaagatc gaagaacgtc gcgccgcgga
    22201 tgacgatgcc gcgcgcgatc cggatgtagt gcgtggccgg cagcaggtac gagacgacgc
    22261 gcagcggcaa cggaagcgat gcgatcggga agatgtatcc cgataggagt accgagggca
    22321 gcatgatccc catggcccgc tgcatcgctt ccatctgcga gttggcgcgc gacgcgacca
    22381 gcaaaccggc cgacagcagt gccagaagat agatcggcga gaggatcagg agcagcgtca
    22441 ggctgccgtg gatggggacc cggaagagga cccgcatcaa aaagagaatc atcaggagtt
    22501 gaaggtaggc caggccgagg tagggaagga gtttgcctgt gacgacggcc atgggcgaga
    22561 ccggcgtgac catgagctgc tcgagcgttc cgcgctcccg ctctttcacg atggcaaacg
    22621 cggaaaggat cgttcccgag aacgtcagca ggatcgagat gagaccagga atgagcagat
    22681 tcgcgctgcg catgtcgggg ttgaacatca tcaccggatg cgcttccacc ggcggcattg
    22741 cgtgactctt ttcgatcagg tcctggattc cattgaagag cacgactccg ttcgcggccg
    22801 aaagcgcctg attggcgatg ctcgagtctg atccgtcgat catgatcagc acgttggcct
    22861 gccggtgcga agcgagattg cgcgcgtagt cgggcggaat ctcgaccgcc acctgcgcat
    22921 gacccgcgac gatctggtgc tgcagctcgg cgcgcgaagc gaccattgcg gtcacatgca
    22981 ggtacgacgt attgccgaac tgctcgatga gccggcggct ctcctgagag cgactctgat
    23041 cgaagatcgc ggttgggatg ttcttggcgt tcatgtcgat gacgccgaag aggatcagct
    23101 cgaacaccgg cactgcaatc gcgaaaaaca gcgttcctcg gtcgcgcagc atctgcgtca
    23161 gttctttgcg gaacatcgca ccgaagccgt tcatcgcgcc gcctcgatct cgcggccgcg
    23221 tgtttcggtc aggcgaacaa agacgtcttc cagcgagggc tcgatcggac tgacgctgac
    23281 ctcgccgagt ttgaaggctt cgagatcgtt ttcgagcttc tcgttggaga cgttgctgtc
    23341 gatca
//
LOCUS       DATLVW010000674         8155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_606956, whole genome shotgun sequence.
ACCESSION   DATLVW010000674 DATLVW010000000
VERSION     DATLVW010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606956"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1513
                     /locus_tag="VNN25_19665"
     CDS             44..1513
                     /locus_tag="VNN25_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXH93807.1"
                     /translation="MNGQWVASASGTTFENRNPADNDDLIGTFQESNEHDLNAAVDAA
                     DRAFQQWRLTPAPLRAEYLYAVANILKRDKEAIASEMTREMGKVLQETRGDVQEAIDM
                     AFLMAGEGRRLHGVTTPSELPNKFNMAVRMPLGVAGLITPWNFPIAIPAWKGMAALIC
                     GNTVVIKPASQTPLSVVMLAEAFEEAGLPKGVWNVVTGGGKAVGEPMLRHPKVSVISF
                     TGSTDVGRQISVACAPQFKHVHLEMGGKNVILVMDDADVDLAVDGALWGAFGTAGQRC
                     TAASRIVAHSKIYDQFVEKLSARANKLRVGNGLDPQTDMGPNISADQIATIAKYVKIG
                     SDEGATVTAGGATLTDGAYSKGHFHQPTVFANVKPDMRIAQEEIFGPVTAVMRCESLE
                     EAISIGNGVKYGLSSSIYTRDINKAFVAMRDMYTGIFYVNAPTIGAEVHLPFGGTKQT
                     GNGHREAGIAGIDVFSEWKSIYVDYSGALQRAQIDTEEV"
     gene            1632..2972
                     /locus_tag="VNN25_19670"
     CDS             1632..2972
                     /locus_tag="VNN25_19670"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH93808.1"
                     /translation="MKSLIGFLLLVSSSAFAQSTADAIIAKELQSSRSYETLSHLTDD
                     IGARLSGSQGAALAVKWATRQFREWEIPVSNEPVKVPHWVRGREEAQLVSPAHHQLVL
                     TAIGGSVATPAAGITADVIEVASFDDLKTLGAAKLRGKIVFYNNPMDMDLVRAHRAFE
                     AYRIAVAFRGAGASRAAEFGAKAIVIRSVGSASLRTPHTGALHYDPKQPRIPAGALTA
                     EDAMLVHRLLARGKRVRMHMVLTPRTLPDVMSANVIAEIRGSEKPEEIVLIGAHLDSW
                     DLATGAIDDGSGVAMVMETMRLLKEMNLHPRRTIRAVLYMNEENGLRGGQQYAKDHGK
                     EKHIAAIETDAGGAAPTGFTTTLKGDALAALESRTKPLAAVSSNHFEVAEETGADTGP
                     LTAAGVPGFGLVPEPLHYFDYHHTNADTLDKVDPKELAQDSAAVAALAWILADN"
     gene            3345..4619
                     /locus_tag="VNN25_19675"
     CDS             3345..4619
                     /locus_tag="VNN25_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HXH93809.1"
                     /translation="MQKDIIGGATTFVAMAYIIVVNPAILKAAGLPVGASTVATIVVA
                     AVGSILMGLYAKRPIAVAPYMGENAFIAFGLGALGITWQQRIGSVFVSGVLFLLITLI
                     GLRGWLAKSISQSLKFSFAAGIGLFLAFIGLTETGIVESGTGVPVKIGNLRSPQVLLA
                     VGGFIVMMALMRLRVRGAILIGIVLTAAAGILLGFGHAPHSFFAMPFTGDYSLAPIAL
                     QLDIRGVLKLSFLPILLTLFLMSFLDTLGTLVGVGAAGGMLDERGNFPKIEKPMTVDA
                     ISCCISGLLGTSTSGAYIESAVGIREGARTGVAAIVTGLLFALSLFFVPLVEPMQQLR
                     FAYGPALIAVGALMLESIRRIDFDDLTELVPAFATIAMMVFTYNIANGLTAGLVLYPL
                     MKLLAGRPREISGGSIVLAALCVVYYLFGLPH"
     gene            complement(4676..7861)
                     /locus_tag="VNN25_19680"
     CDS             complement(4676..7861)
                     /locus_tag="VNN25_19680"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH93810.1"
                     /translation="MKLQRFTICFFLLLLVAAGAFAQGTTGALTGTVTQAGTNLPGVT
                     VTISSPTLQGTRTTVTNENGDYNFSAIPPGQYKVVFSLSGLSDVTKNATVNLVETKRL
                     DADMKMSAVSEAITVTASQATVLETPQIESNYKQEAVNKLPINRNPAAQATLSPGVSA
                     GINGNTISGAMSYDNLYLVNGAVVNENLRGQPHALYIEDAIQETTVLTGGISAEYGRF
                     TGGVVSSITKSGGNEFSGSVRDSLDNPQWTEKSLPSQTVPIDHTNNTYEETLGGRIIR
                     DRLWFFVAGRQSKISANRSISAVTGGGGLPNPTIAAYTVNTSNPRQEYKVTANLTQKH
                     SIVGSYLNNDQKSDKNCQGTCIDLRTLNTTGVDNPNNFKTLHYNGVITNNLLLEGDWA
                     KKYFAFVGFGGSQPQGATSAHDLATGTTLRDNFTGQGGTGNAPLFCGSCSPELRNNKE
                     WEAKARYFLGTKSLGTHNMVIGYDDWAEQRLSNNYQSTTDFRFNVSGAAPVQQPDGTI
                     KVQLTSNVDSFSWFPIAFASLGSNLKTTSVFANDKWDLNQHLTFNLGARYDKNDAVDS
                     AGNGISKDSLVSPRVGAIFDVFGNGRLRVDANYGIYSGRLAETVSGSASAAGNPASYL
                     FVYGGPSTGLLDSVTALEQAFTWFLANGGTNRTFASASIPGLNTKLDPSGIKSPNMRE
                     FTVGAGTQIGKGYVRADYINRDWRDFYAQFDSLALGTVVGNPFTGALADLNIIGNSDR
                     PVRKYWAVQTQAQYQLPAGFGAGGNYTYSKLTGNVEGENTGSGPITFANLDVAYQEYQ
                     NFKQNAPTGYLAADERHKVRAWGTYDIGTSIGHFNFGLIERYDSGSPYSAAGSIPITN
                     AMLCSGIPTATGTTVTSYNTCSYKLADNTTGTGTPIYPNGATRNTASQYAPNGNSSTA
                     TTTSGLPARAFLGPTSVTYFFSPRGAFKTNSIISTDVAVNYELPIRNFAFFAKAEVRN
                     LLNHRSSVSVVTTVRTNSTAGSGLLAFNPFTQSPIECPKGAPAATCTALGANYQLAST
                     FGTSNTTPTTFSQAGSFQLPRTYVYAIGARF"
BASE COUNT         1507 a         2304 c         2682 g         1662 t
ORIGIN      
        1 gcccaacaca gcactgcgcc caacgcgcga ctacaagaac tacatcaatg gccagtgggt
       61 cgcttctgcg agcggaacga ccttcgagaa ccgcaatccc gccgacaacg acgatctcat
      121 cggcacgttt caggagtcga atgagcacga tctgaatgca gccgtcgacg ccgcagaccg
      181 agcgtttcag cagtggcggc tgacacccgc accgctccgc gccgagtacc tctatgccgt
      241 cgccaacatc ctcaagcggg acaaggaagc gatcgcatcc gagatgactc gcgagatggg
      301 gaaggtgctg caggaaacgc gcggcgatgt gcaggaagcg atcgacatgg ccttcctgat
      361 ggccggcgag ggacgccgcc tgcacggcgt gacgactcct tccgagttgc ccaacaagtt
      421 caacatggcc gttcgcatgc ccctcggcgt cgccggtctg atcacgccct ggaactttcc
      481 gatcgccatc cctgcgtgga aaggaatggc tgcgctgatc tgcggcaaca cggttgtcat
      541 caagccggca tcgcaaacgc cactgtccgt ggtgatgctc gcggaagcgt tcgaagaggc
      601 cggactgccg aaaggtgtct ggaacgtcgt caccggcggc ggcaaagcgg ttggcgagcc
      661 gatgctgcgg cacccgaaag tatcggtgat ctcgttcacc ggctcgaccg acgtggggcg
      721 ccagatctcg gtcgcctgcg cgccgcagtt caaacacgtc catctcgaga tgggcggcaa
      781 gaacgtgatc ctggtcatgg acgacgcgga tgtcgatctc gcggtcgacg gcgcgctttg
      841 gggtgcattc ggtacggctg gtcagcgttg cacggccgcg tcacggatcg tggcgcactc
      901 caaaatctac gatcagttcg tggagaagct ctccgcgcgc gcgaacaaac tacgcgtcgg
      961 caacggactc gatccgcaga cggacatggg accgaacatc tcggcggacc agatcgcaac
     1021 gatcgcgaag tacgtgaaga tcggcagcga cgagggagcg acggtcacag cgggcggcgc
     1081 cacgttgacg gatggcgcgt acagcaaggg tcattttcat cagcccaccg tgttcgcgaa
     1141 cgtcaagccg gacatgcgca tcgcacagga ggagatcttc gggccggtga cggcggtgat
     1201 gcgctgtgag tcgctcgaag aggcgatctc gatcggcaac ggcgtgaagt acgggttgtc
     1261 gtcgtcgatc tacacccgcg atatcaacaa ggcgttcgtg gcgatgcgcg atatgtacac
     1321 cggcatcttc tacgtgaacg cgccgacgat cggtgccgag gtccatctgc cgttcggtgg
     1381 gacgaagcag acgggcaatg gacaccgcga ggcgggtatc gctggcatcg acgtgttcag
     1441 cgagtggaag tcgatttacg tcgactactc cggtgcactg caacgggcgc agatcgatac
     1501 ggaagaggtg tagggcaggt tcaccacaga gacacagagg gcacagagat acaactgaaa
     1561 cctctgtgct ctctgtgtct ctgtggttga atcgtctcca gccacaattg ttccgctaca
     1621 cttcgcgggc aatgaaaagc ctcatcggat ttctgctgct cgtttctagc tctgcgttcg
     1681 cgcaatcgac cgcagatgcg atcatcgcga aagaactgca atcgtcacgt tcgtacgaaa
     1741 cgctgagtca tctcaccgac gacatcggtg cgcgtttgag cggctcgcaa ggcgcggcgc
     1801 tggcggtgaa atgggcgacg cggcagttcc gtgagtggga gatcccggtg tcgaacgagc
     1861 cggtcaaagt gccgcattgg gtgcgtggcc gtgaggaggc gcagctcgtg tcgccggcgc
     1921 atcatcaact cgttctgact gccattggcg gcagcgtggc gacgccggca gcagggatca
     1981 ctgccgacgt tatcgaagtc gcttcgttcg acgatctgaa gacactcggc gctgcgaaac
     2041 tgcgcggcaa gatcgttttc tacaacaacc cgatggacat ggatctcgtt cgggcgcatc
     2101 gcgcgttcga ggcgtaccgc atcgccgtcg cctttcgggg cgccggtgcc agccgtgccg
     2161 cggagttcgg cgcgaaggcg attgtgattc gctcggtcgg atcggcctcg ctgcggacgc
     2221 cgcacaccgg agcgctgcac tacgatccga agcagccgcg gattccggcg ggagcgctga
     2281 cggccgagga tgccatgctc gtgcaccggc tgctggcccg gggaaagcgc gtgcgcatgc
     2341 acatggtcct cacgccacgg acgcttcctg atgtgatgtc ggcgaacgtc atcgccgaga
     2401 ttcggggcag cgagaagccg gaggagatcg tcctcatcgg cgcgcacctc gattcgtggg
     2461 acctggcaac gggcgcgatc gacgacggct ccggcgtggc gatggtgatg gagacgatgc
     2521 ggctattgaa agagatgaac cttcatcccc gccgtacgat tcgcgccgtc ctctacatga
     2581 atgaagagaa cggtctgcgt ggcgggcagc agtacgcgaa ggatcatgga aaagaaaagc
     2641 acatcgccgc gatcgagact gatgccggcg gcgcggcgcc gaccggcttc accacgacac
     2701 tgaaaggcga cgcactggct gcgctggaat cgagaacgaa gccgctggcg gcggtctcgt
     2761 ccaaccactt cgaggtcgcc gaggagacgg gggcggacac cggcccgctg acggctgcgg
     2821 gcgtgccggg attcggcctt gtgccggagc cgctgcacta cttcgactac caccatacga
     2881 acgccgacac gctcgacaaa gtcgatccga aggagcttgc gcaggattcg gcagcggtgg
     2941 cggcgctggc gtggatattg gcggacaact agtcgttttt catcggtccg cgaaaacgcc
     3001 gggcatcatc aatcgtgtca tcctgagaca gccggagccg ccatctgcgg tcgatcgatt
     3061 tgcgtagcgt ggagtggcgg cgtaggacgg cgaaggatct caaaatgcgc cgcatgtagt
     3121 gtgtgcatcg taattagaga tccttcgccg tcccacgccg ccacttcact cgtcgtgagt
     3181 tgatcgagct cggatggcgg ctccggcagt ctcaggatgg caccaaggat gacaccaatc
     3241 tctttcggct gcgaaagaga ggatggcact aacgtcgtca tcgtcaatca atcgcgatgt
     3301 atcgatccgc actttccatc caggcgctca ggtaaagttc cgtcatgcag aaagacatca
     3361 tcggcggcgc gacgacgttc gtcgcgatgg cttacatcat cgtcgtcaat ccggcgattc
     3421 tgaaggcggc ggggttgccg gtgggggcga gcaccgtggc cacgatcgta gttgcggctg
     3481 tgggctcgat tctcatgggt ctctacgcga agcggcccat cgcggtggcg ccgtacatgg
     3541 gagagaacgc cttcatcgcc tttggactcg gcgcgctcgg gatcacgtgg cagcagcgca
     3601 tcggatcggt ctttgtcagc ggcgttctct tcctccttat cacgctgatc gggctgcgcg
     3661 gctggcttgc gaagtcgatc tcgcagagcc tgaagttcag tttcgccgcg ggcatcggcc
     3721 tcttcctcgc gttcatcgga ttgacggaga cgggtatcgt cgagagtgga acgggagtgc
     3781 cggtgaagat cggcaatctg cgcagcccgc aggtactgct ggccgtcggc ggattcatcg
     3841 tgatgatggc gctgatgcgg ttacgcgttc gcggtgccat cctgataggc atcgtcctca
     3901 ccgccgccgc cggcatcctc ctcggcttcg gtcatgcccc gcactcgttc tttgcgatgc
     3961 ccttcacggg cgattacagc ctggcgccga tcgcgctgca gctcgacatt cgcggggtac
     4021 tgaagctgtc gtttctgccg atcctgctga cgctttttct gatgagcttt ctcgacacgc
     4081 ttggaacgct cgtcggcgtc ggtgccgcgg gcgggatgct cgacgagcgc ggcaattttc
     4141 cgaagatcga gaagccgatg acggtcgatg ccatttcgtg ctgcatcagc ggcctgctgg
     4201 ggacgtcgac gagcggcgcg tacatcgagt cggcggttgg catccgcgaa ggagcgcgca
     4261 cgggcgtggc agcaatcgtg accggtctcc ttttcgcgct gtcactcttt ttcgtcccgc
     4321 tggtggagcc gatgcagcag cttcgctttg cgtacggtcc ggcgctgatt gcggtcggcg
     4381 cgctgatgct cgagtcgatc cgccgcatcg acttcgacga tctgacggag ctggtgccgg
     4441 cgttcgcgac gattgcgatg atggtcttca cctacaacat cgccaatggt ctgactgcag
     4501 gcttggtgct gtacccgttg atgaagcttc tggcgggacg gccgcgcgag atcagcgggg
     4561 gatcgattgt gcttgcggcg ctgtgtgtcg tttactacct gttcggtctt ccgcactgaa
     4621 acaaaaagcc ccggccgagg ccggggcttt cgttacaaac catgttgcgg gatgactaga
     4681 agcgcgcgcc gattgcgtac acgtaggtgc gcgggagctg gaagctaccg gcctgtgaga
     4741 aggtcgtcgg cgtagtgttt gacgttccga aggtggaggc aagctgatag ttcgctccca
     4801 gcgcggtgca ggtggctgcg ggagcgccct tcgggcattc gatcggagac tgcgtgaacg
     4861 ggttgaacgc gagcaacccc gaacctgcgg tggaattcgt ccggacagtc gtcacaacgc
     4921 tgacactcga acggtggttc agcaggttcc gaacttctgc cttggcaaag aacgcaaagt
     4981 tccggatggg cagctcatag ttcacggcta cgtccgtgga gatgatgctg ttcgtcttga
     5041 aggcgccgcg agggctgaag aagtaggtaa cggaagttgg gccgaggaac gctcttgccg
     5101 gaagtccgct cgtggttgtt gccgtcgatg aatttccgtt aggcgcgtac tggctcgccg
     5161 tattacgggt agcgccgttc ggataaatcg gggtcccggt gcctgtcgtg ttgtcggcga
     5221 gcttgtacga gcaggtgttg tagctggtga cggtagtgcc cgtcgcggtt ggaatcccgg
     5281 agcacagcat ggcattcgtg atcggaatgg agccggcggc ggagtacggg ctgcccgagt
     5341 cgtagcgctc gatcaggccg aagttgaagt gtccgatcga cgtaccgatg tcgtacgtgc
     5401 cccatgcgcg cactttgtgg cgctcgtcgg cggcgaggta gccggttggc gcattctgct
     5461 tgaagttctg gtactcctgg taggccacgt ccagattggc aaacgtgatc ggaccgctgc
     5521 cggtgttctc accttcgacg ttgccggtaa gcttggagta cgtgtagttg ccacccgcgc
     5581 cgaaaccggc gggaagctgg tactgagcct gcgtctggac ggcccagtac ttacggaccg
     5641 gccgatccga gttgccgatg atgttgaggt cggccagagc accggtgaat ggatttccga
     5701 cgacagtgcc cagggccagg ctgtcaaact gggcgtagaa atcgcgccag tcgcggttga
     5761 tgtaatccgc gcggacgtag cctttcccga tctgcgtgcc cgcgccgacg gtgaactcgc
     5821 gcatgttggg ggacttgata ccggagggat cgagcttggt gttcagtccc ggaatgctgg
     5881 cggaagcgaa ggtgcggttg gttccaccgt tggcgaggaa ccaggtgaag gcttgctcga
     5941 gggcagtcac tgaatcgagc agaccggtgc tgggaccgcc gtagacaaac aaatagctag
     6001 cggggtttcc ggctgcggag gccgagccgg acacggtctc ggcgaggcgg ccgctgtaga
     6061 ttccgtagtt ggcgtctacg cgcaggcggc cattgccgaa gacgtcgaag atcgcgccga
     6121 cgcgcgggct gactaggctg tctttggaaa tgccattgcc ggcgctgtcg acggcgtcgt
     6181 tcttgtcgta gcgggcgccg aggttgaatg tgaggtgctg gttgaggtcc cacttgtcgt
     6241 tggcgaaaac tgaggtggtc ttcaggttcg agccaagcga tgcgaaggcg atcgggaacc
     6301 aggagaaact atcgacgttg ctggttagct ggaccttgat cgtgccgtca ggctgctgca
     6361 ccggcgccgc gccgctgacg ttgaaacgaa aatcggtggt ggattggtaa ttgttcgaga
     6421 gccgctgctc agcccaatcg tcgtaaccga tgaccatatt gtgggtgccg agcgacttgg
     6481 tacccaggaa gtagcgagcc ttcgcttccc actctttgtt gttgcgaagc tcgggactgc
     6541 agcttccgca gaagagcgga gcattgccgg ttccaccctg cccggtgaaa ttgtcgcgca
     6601 gggtcgtacc ggtagcgagg tcatgagcgc tggtggcgcc ctgaggctgg ctgccgccga
     6661 agccgacgaa cgcgaagtac ttcttcgccc agtcgccttc gaggaggagg ttgttggtga
     6721 tgacgccgtt gtagtgcagg gtcttgaagt tgttcgggtt atcgacaccg gtcgtgttca
     6781 gcgtacgcag atcgatgcaa gtgccctggc agttcttgtc tgatttctga tcgttgttga
     6841 gatacgaccc gacgatcgag tgcttctgtg tcaggtttgc ggtgactttg tattcctgac
     6901 gtgggttgct cgtgttcacc gtatacgcgg caatggttgg attcggcaga ccgcctccac
     6961 ccgtcacggc tgaaatcgag cggttggccg agatcttcga ctggcggccg gccacgaaga
     7021 accacagacg gtcgcgaatg atgcggccac cgagtgtttc ttcgtacgtg ttgttggtat
     7081 ggtcgatcgg gacggtctgc gagggcagac tcttctccgt ccactgagga ttgtcgagcg
     7141 agtcgcggac ggagccgctg aactcgttgc cgcccgactt ggtgatcgaa gaaaccaccc
     7201 cgccggtgaa gcggccgtat tctgcggaga tgccgccggt gagaaccgtc gtctcctgaa
     7261 tggcgtcttc gatgtagagg gcgtgcggct ggccgcgaag gttctcgttc acgacagcgc
     7321 cgttgaccag ataaaggttg tcgtacgaca tcgcgccgga gatcgtgttg ccgttgattc
     7381 cggcactgac gccgggcgac agggtagcct gcgctgccgg attgcggttg atgggcagtt
     7441 tgttgactgc ctcttgtttg tagttcgact cgatctgagg cgtctccagc acggtggcct
     7501 ggctggccgt gaccgtgatg gcttcggaga cggccgacat cttcatgtca gcatcgagac
     7561 gtttggtctc aacgaggttg accgtcgcgt tcttcgtgac gtcgctcagc ccggacagcg
     7621 agaacaccac tttgtactga cctggcggga tcgccgaaaa gttgtagtcg ccgttctcat
     7681 tcgtgacggt cgtacgtgtt ccctgcagtg tcggcgacga gatcgtcacc gtcacgcccg
     7741 gaagattggt tcccgcttgc gtgaccgtgc cggtcagcgc cccggtcgtg ccctgcgcga
     7801 aggcgccggc tgcaaccagg agcagcagga agaaacagat cgtaaatcgt tgtagcttca
     7861 tcgttcctcc tcgattttcc ctcaagtgaa tattactgtt ggacttcaaa cgtgccgtgc
     7921 atttgccgaa ccacttatgc aaacggattc ttggatctca agtcactgca aaataagggt
     7981 ttgcttcatc aaacggtcgg ctctcggaac ggagtgattc aacggattga acacacgcaa
     8041 cggttcgccg catcaaagcc cggccacgat tccgtgtggc gcatccgcga aagatttcct
     8101 cgttcagaaa gacaattgtt gggcactgat tggagcggtt ggacagccgc cgttt
//
LOCUS       DATLVW010000675         7529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_154915, whole genome shotgun sequence.
ACCESSION   DATLVW010000675 DATLVW010000000
VERSION     DATLVW010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154915"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..621)
                     /locus_tag="VNN25_19685"
     CDS             complement(127..621)
                     /locus_tag="VNN25_19685"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXH93811.1"
                     /translation="MESQEKFIVLLRHGIAEPHGTREDDDARILTETGNRRMKQIGRG
                     LAKCFPKAEAIYSSPLIRCIETAEWVEKGYGSAVHMKTTDALKPGADVEAFRALLKKI
                     KARHVIFVGHEPNLSTTMLAIASLHADSPIELKKGGCYGLRLAADGSAHLEWVLPPRV
                     LRRR"
     gene            complement(633..2162)
                     /locus_tag="VNN25_19690"
     CDS             complement(633..2162)
                     /locus_tag="VNN25_19690"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HXH93812.1"
                     /translation="MAEKPKRIAAIDVGTNSIHMIVAETRDRGYRVVDREKEMVQLGL
                     ASLDGEPLTEDAMVRGVASIGHMAEIAAKWNVDEVFAVATSAVREAPNRRDFLKRVKD
                     TTGVKVRIISGEEEADYIFRAVRSAVDVDGQTALCIDIGGGSVEMIVGTADETYFTRS
                     EPLGALRLAQRFELTERPSPRALDECRNHIHDRIAKLRRRIRRLGVDLCAGTSGTIQT
                     LATMASPADTQSSTHGLRRLERAAVQNLIPVLASMTSRERAERFSLDAPRATSILAGA
                     VVLATLLETFDIEEMLACPLAMREGMLESRVAALEKPDDIGGSLRYASVLALAQRTDV
                     DLKHGTHVAKLAARIFDQTKALHALEAKTTDLLESAAVLHEAGLHVSDRAHHKHSYYL
                     IRHADLRGYTDEEILIIANTARYYRKAPPAPSHDNFAELTHTQQQDVEKLAAILRIAE
                     ALDRGHRRSVRDVAVRVTREQATFTVRTRSDSAVEIASARKRAKYFADLFEVEVRFEA
                     M"
     gene            2246..3046
                     /locus_tag="VNN25_19695"
     CDS             2246..3046
                     /locus_tag="VNN25_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HXH93813.1"
                     /translation="MHTKVHRIDPGSKVRMKDFDPEGTPLCNDKADGKERCVDLESRL
                     GELQELLYAEHKHKVLIILQGMDTSGKDGTVRHVLNGMSLSGVRVVSFKKPTQEELDH
                     DFLWRVHAPMPGEGEVVVFNRSHYEDVLVVRVHNLVDEKVWRKRYKQINEFEKMLAAT
                     GTTILKFFLNISKNEQRKRLQERIDDPKKHWKFQHGDLEERKLWDKYMDAYEDVLERT
                     STKWAPWHVIPANAKWYRNLVVAEIIKDALESLKMEYPVIDLSGEVVE"
     gene            complement(3231..3974)
                     /locus_tag="VNN25_19700"
     CDS             complement(3231..3974)
                     /locus_tag="VNN25_19700"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HXH93814.1"
                     /translation="MFRRIANLFRGFLSLFIGGVERQNPEALLEVEKENIRRQIANYN
                     QGLAAHAGLCERLMTQVKRLEQEERELRAKTAANLRAGNRDLASQQALRLQTVQRELI
                     ENRTQLESAEGTYKELLRARDVSVKAAQAKIESLKTSLNDLKIKRATAELNEMASGMI
                     SQIGGSGDTLNRLHEMLEEEREKAAGRARVARDTLHSTDFEMQEHEQKALADQALADF
                     AANEGLALEPPKAVKAIAAAEQKSLGPKT"
     gene            4104..5210
                     /locus_tag="VNN25_19705"
     CDS             4104..5210
                     /locus_tag="VNN25_19705"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXH93815.1"
                     /translation="MKIRNLAARFIAAAAIVVSTTGAFAQVTPAEGVTPPDDTPKFNV
                     GATIYGDYTYADSPTTKDSDGNVIHASAFNITRAYINVTGNINHWILFRITPDVSRET
                     STGPSLSGSQIFRLKYGYAQLNLDDWTTKGSWVRFGQQQTPYLDYSEGIYRYRFQGTM
                     FAERAGLISSADSGISGHYAFNGNYGDVHAGFYNGENYNKAEVNNEKGFEVRGTVRPF
                     PLGGPELKGLRLTGFVIDDHYVGSAKRQRTIGQVTYEHALGNAGFEVLRAKDQTSASK
                     PQISSKGWSVWVTPKLGTQGWEALIRHDNFLPNDAIHSQKQKRDIDGIAYWVPGLSGK
                     AVAFLFDRDSLKRSGLTPAAPNATNYEMKMLVNF"
     gene            5220..6230
                     /gene="pstS"
                     /locus_tag="VNN25_19710"
     CDS             5220..6230
                     /gene="pstS"
                     /locus_tag="VNN25_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXH93816.1"
                     /translation="MKKLFLAAALLLATAASAQTVQINGAGATFPSPIYTKWFDQYHK
                     AHPNAEINYQPIGSGGGIRQLSAGTVFFGASDGPMTNDQITGAGFRILHLPTVLGGVV
                     PIYNIPEVKAELRFPGKVLADIYLGKITRWNDSAIQAANPGVSLPGSEITVVHRSDGS
                     GTSYIWCDYLSKVSPEYRKTVGVATSVNWPAGVGAKGNEGVAGLVKQTPSSIGYVELI
                     FALQNKMSYGTVQSQAGEFVRASTETVTNAAAEAVKNMPKDFRVSITNAPGRNVYPIS
                     SFTWLLLQQSPRDVVRSRMMVEFARWALTDGQQYAAPLGYAPLPKEIVAMEMEALKQV
                     KQ"
     gene            6369..7406
                     /gene="pstS"
                     /locus_tag="VNN25_19715"
     CDS             6369..7406
                     /gene="pstS"
                     /locus_tag="VNN25_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXH93817.1"
                     /translation="MKRTLFAVALSVALAACAPQENGNNTTTSTGPLAINGAGATFPN
                     PIYSKWFDEYQKSHPEVHINYQPIGSGGGIKQLTAQTVFFGASDGPMTDAQLKDAPAP
                     ILHFPTVLGGVVPVYNVPGVTQPLRFSGPVLATIYLGRITKWNHPAIAQLNPGVALPD
                     TDIAVVHRSDGSGTTYIFCDFLSKVSPDFKEKVGVATSVNWPAGVGAKGNEGVSGLVQ
                     QTPGAIGYVELIYALQNKIPYGPVQNKSGKFITASLDSVTAAAAGVTMPDDFRVSITD
                     PAAADAYPIASFTWMLLYQHPADKARSKAMVDFLKWALADGQKFAPGLGYAPLPQPVV
                     AKELQALATVQ"
BASE COUNT         1396 a         2460 c         2262 g         1411 t
ORIGIN      
        1 gattctcggg gtcaacctcg acattcttgc caacgatatc gacctcgaac caactcgtct
       61 caacctgact ttgacgattc cttcacgtca agcgcggcgc atcgattcac gacgaaagca
      121 cttctctcac cgccttcgca aaacgcgcgg cggcagcacc cactccagat gcgcactgcc
      181 atcggcagcc agtcgcaagc cgtagcagcc gccctttttg agctcgatcg ggctgtcggc
      241 gtgaagactc gcgatcgcca gcatcgtcgt actcagattc ggttcgtggc caacgaagat
      301 gacgtgacgc gccttgatct tcttgagcaa cgcacgaaat gcttcgacat cggcgccagg
      361 cttcagcgcg tccgtcgttt tcatatgcac ggcagatccg tagcctttct ccacccactc
      421 cgccgtctcg atgcagcgga tcaacggact cgaatagatc gcctcggcct tcggaaagca
      481 tttcgccagt ccgcggccga tctgcttcat ccgccggttg ccggtctcgg taagaatgcg
      541 cgcgtcatcg tcttcgcgtg tgccgtgcgg ctcggcgatt ccgtgacgaa gaaggacgat
      601 gaacttttcc tgagactcca cggcgtgcct cctcacatcg cttcgaagcg gacttctact
      661 tcgaagaggt cggcgaagta tttcgctcgt tttcgtgcgc tggcaatctc gaccgcggaa
      721 tcagaccgcg tgcgaacggt gaatgtcgcc tgctcgcgcg tgacgcgcac cgccacatcg
      781 cgcaccgagc gccggtgccc gcggtccagc gcctcggcga tccgcaaaat ggcggcgagt
      841 ttctccacgt cctgctgctg cgtgtgtgtc agctcggcga agttgtcgtg cgacggcgca
      901 ggaggcgcct tccggtagta ccgcgcggtg ttggcaatga tgagaatctc ctcgtctgta
      961 tagccgcgca ggtcggcgtg gcggatgagg tagtagctgt gcttgtgatg cgcgcggtcg
     1021 ctgacgtgca gcccggcttc gtgcagcacg gccgctgatt cgagcagatc ggtcgtcttc
     1081 gcttccagcg cgtgcagcgc cttggtctga tcgaagatgc gcgcggccag cttggcgacg
     1141 tgcgtgccgt gtttcagatc gacgtcggtg cgttgcgcga gcgcgagcac cgaggcgtag
     1201 cggagggagc cgccgatatc gtcgggcttt tccagcgccg ccacgcggct ttcgagcatc
     1261 ccttcgcgca tcgccagagg gcaggccagc atctcctcga tgtcgaacgt ctccagcaac
     1321 gtggccagca caacggctcc ggcgaggatg ctggtggccc gaggcgcgtc gaggctgaac
     1381 cgctcggcgc gctcgcgcga ggtcatcgac gcgaggacgg ggatgaggtt ctgtacggca
     1441 gcccgctcca gccggcgcag accatgagtc gaggactgcg tgtcggccgg cgacgccatc
     1501 gtggcaagag tctgaatcgt tccggatgtc ccggcgcaga gatcgacacc gaggcggcgg
     1561 atgcggcggc gaagcttggc gatgcggtcg tggatgtgat tgcggcactc atcgagcgca
     1621 cgcggcgacg gacgctccgt caattcgaag cgctgcgcca gacgaagcgc gccgaggggc
     1681 tcgctgcgcg tgaaataggt ctcgtccgcg gtgccgacga tcatctccac gctgccgccg
     1741 ccgatgtcga tgcaaagggc tgtctgcccg tcgacgtcga ccgccgaacg aacggcgcgg
     1801 aagatgtagt cggcctcttc ttcgccggaa atgatccgca ctttcacgcc ggtcgtgtcc
     1861 tttacgcgct tgaggaagtc gcgtcggttc ggcgcttcgc gcacggcgct cgtggccacc
     1921 gcgaagacct cgtcgacgtt ccacttggcg gcaatctctg ccatgtgtcc gatcgaggcc
     1981 acgccgcgaa ccatggcgtc ctcggtcagc ggttcgccgt cgaggctggc cagaccgagc
     2041 tgcaccatct ccttctcgcg gtcgacgacg cgatagccgc ggtcgcgcgt ctccgccacg
     2101 atcatgtgga tcgagttcgt gccgacgtcg atggcggcaa tccgcttcgg tttttcagcc
     2161 atgctgcaac gcagtatcct cgccctcacc ggtttgagga tgaagagctt gtaaagaatg
     2221 cctgttccgg gaggagaatc gcacaatgca caccaaagtc catcgtatcg atcccggcag
     2281 taaagtccgc atgaaggatt tcgatcccga gggcacgcca ctctgtaacg acaaggccga
     2341 tggcaaggaa cgatgcgtcg acctcgaaag ccgcctcggc gagctccagg agttgctcta
     2401 cgccgagcac aaacacaaag tcctgatcat cctccagggg atggatacct cgggcaagga
     2461 tgggacggtc cgccacgtcc tgaacggcat gagcctgtcg ggcgtgcgcg tggtctcgtt
     2521 caagaagccg acgcaggaag aactcgacca cgactttctc tggcgcgtcc acgcaccgat
     2581 gccgggcgag ggcgaggtcg tggttttcaa tcgcagccac tacgaggacg tgctcgtcgt
     2641 gcgggtgcac aacctggtcg atgagaaggt ctggcggaag cggtacaagc agatcaacga
     2701 gttcgagaag atgctggcag ccaccggtac gacgatcctc aagttcttcc tcaacatcag
     2761 caagaatgag cagcgcaaac gcctgcagga gcgcatcgac gatccgaaga agcactggaa
     2821 gttccagcac ggcgacctcg aggagcgcaa gctttgggac aagtacatgg acgcctacga
     2881 agacgtgctc gaaaggacgt cgacgaaatg ggcgccctgg cacgtgatcc ccgcgaacgc
     2941 aaagtggtac cggaacctgg tcgtcgcgga gatcatcaag gacgcattgg agtcgctgaa
     3001 gatggagtac ccggtgatcg atctgtcggg cgaggtggtg gagtaggagt gcgggcgtct
     3061 cgaccgcggg cactggggcg tctcgcccaa gtgcttggct ggtcgcgagt cgagttggcc
     3121 cgcgcaccgc ccggacgccc aactgaggtt cgagcttcga tatcagtcgc gaattgcaaa
     3181 ccatcggggc ggggacgccc caatggccgc gggcgagacg cccacactcc tcatgtcttc
     3241 ggccccaaac tcttctgctc cgccgccgcg atcgccttca cggccttcgg cggttccaac
     3301 gccagcccct cgttcgccgc gaagtccgcc agcgcctgat cggcgagcgc cttctgctcg
     3361 tgctcctgca tctcgaaatc cgtggagtga agcgtgtcgc gggcgacgcg ggcgcggccg
     3421 gcggctttct cgcgctcttc ttccagcatc tcgtgcaggc ggttgagcgt gtcgccggag
     3481 ccgccgatct gcgagatcat ccctgacgcc atctcgttca gctcggccgt ggcgcgcttg
     3541 atcttcagat cgttcaggct cgtcttgagc gactcgatct tcgcctgcgc tgccttcacc
     3601 gacacgtcgc gcgcgcgcag caactccttg tacgtgcctt cggccgattc gagctgcgtc
     3661 cgattctcga tcagctcgcg ctgtaccgtc tgcagccgca aggcctgctg actggccaga
     3721 tcgcgattgc cggcgcggag gttggctgcc gtcttggccc gcagctccct ctcttcctgc
     3781 tccagccgtt tgacctgcgt catcagccgc tcgcagagac cggcgtgcgc ggccaggccc
     3841 tggttgtagt tcgcgatctg gcggcggatg ttctccttct ccacttcgag aagcgcttcc
     3901 ggattctggc gctccacccc gccgatgaag agactcagga atccgcggaa caggttggcg
     3961 attcgtcgga acattcagct ctcctctgac ccggacatca tgcgccgttt tttcgcgccg
     4021 ttcacgctga gtttacgcgc gccgcatgcc gagtgcaggc cccaaaccta ccatccgggc
     4081 aatcaacagg aggcttgcag gtaatgaaga ttcgcaattt ggcagcccgt ttcatcgccg
     4141 cggcggcgat cgtggtcagc accaccggcg cgtttgcgca ggttacgccg gcggaaggcg
     4201 ttacgccgcc ggacgatacg ccgaagttca acgtcggagc gacgatctac ggcgactaca
     4261 cgtacgccga ttccccaacc acgaaagact cggacggcaa cgtcattcat gccagcgcgt
     4321 tcaacatcac ccgcgcctac atcaacgtca cgggcaacat caatcactgg atccttttcc
     4381 gaatcacgcc ggacgtgtcg cgcgagacga gcacggggcc gtcgctcagc ggcagccaga
     4441 tcttccgtct caagtacggc tacgcgcagt tgaatctcga cgactggacg acgaaaggct
     4501 cgtgggtgcg gttcggccag cagcagacgc cgtacctcga ctacagcgag ggcatctacc
     4561 gctatcgttt ccaggggacg atgttcgcgg agcgggccgg cctgatctcg tcggcggatt
     4621 ccgggatctc cggccactac gccttcaacg gcaactatgg tgacgtgcac gccggcttct
     4681 acaacggcga gaactacaac aaggccgagg tcaataacga gaagggtttc gaagtacgcg
     4741 gcaccgttcg ccctttcccc ctcggcggac cggaactgaa gggcctgcgc ctcacgggct
     4801 tcgtcatcga tgaccactat gtcgggagcg ccaagcggca acgcacgatc ggacaggtca
     4861 cgtacgagca cgccctcggc aatgccggct tcgaagtttt gcgcgcgaag gaccagacgt
     4921 ctgcgtcgaa gccccagatc agttccaaag gctggtcggt ctgggttacg ccgaagctcg
     4981 gcacgcaggg gtgggaagcg ctgatccgtc acgacaactt ccttcccaac gacgccattc
     5041 attcacagaa gcagaagcgc gacatcgacg gcatcgccta ctgggtgccg ggcctgagcg
     5101 gtaaagccgt tgcgttcctg ttcgaccgcg attctctcaa acgttcgggt ctcacgcccg
     5161 cagcccccaa cgccacgaac tacgagatga aaatgctcgt caatttctag gagatgacaa
     5221 tgaagaagct cttcctcgca gccgccctgc tgctcgctac cgctgcatcc gcgcagaccg
     5281 ttcagatcaa cggcgccggc gcgacgttcc ccagcccgat ctatacgaag tggttcgatc
     5341 agtaccacaa ggcgcatccc aatgcggaga tcaactacca gccgatcgga tcgggcggcg
     5401 gcatccgcca gctctccgcc ggcaccgtct tcttcggtgc ctcggatggt ccgatgacga
     5461 acgatcagat caccggcgcc ggctttcgca tcctgcacct gccgaccgtg ctgggtggtg
     5521 tcgttccgat ctacaacatc cccgaggtca aagccgagct ccgcttcccc ggcaaagtcc
     5581 tcgccgacat ctacctcggc aagatcacac gctggaacga ttctgccatt caggccgcga
     5641 atccaggggt cagcctcccg ggcagcgaaa tcaccgtcgt tcaccgctcc gacggctcgg
     5701 gcactagcta catctggtgc gactacctct cgaaggtgtc gcccgagtac cgcaagacag
     5761 tcggtgtggc cacgtcggtg aactggccgg ctggcgtcgg cgccaagggc aacgaaggag
     5821 tagccggtct cgtcaagcag acgccgtcgt cgatcggcta tgtcgagttg atcttcgcac
     5881 tgcagaacaa gatgtcctac ggcacagtgc agagccaggc cggtgagttc gtgcgggcat
     5941 cgaccgagac ggtcacgaat gcggcggccg aggcggtgaa gaacatgccg aaagacttcc
     6001 gcgtgtcgat caccaacgcg cccggccgca acgtctatcc gatctcctcg ttcacctggc
     6061 ttctgctgca acagtcaccg cgcgacgtcg tgcgctcgcg gatgatggtg gagttcgcgc
     6121 gctgggcctt gaccgatggc cagcagtatg ccgcgccgct cggctacgcg ccgctgccga
     6181 aggaaatcgt ggcgatggag atggaggcgc tcaagcaggt gaagcaatag ggtctctcaa
     6241 ggccgatcac cacggagaca cagagactcc tctgtgacct ttctgtctct gtgctgatct
     6301 tcgtctcttc acaccgaatt tacacgccct gcaccgcccc gccaatgcgt gcccgtaacc
     6361 tccgcgccat gaaacgaacg ttgttcgcag tagccctatc agttgcgctt gccgcctgcg
     6421 cgcctcagga aaacggcaac aacacaacca catctaccgg tcccctggcc atcaacggcg
     6481 ccggcgctac cttccccaat cccatctact ccaagtggtt cgatgagtac cagaagtcgc
     6541 atccagaggt tcacatcaac taccagccga tcggttccgg cggtggcatc aaacagttga
     6601 cggcgcaaac ggttttcttc ggcgccagcg acggcccgat gaccgatgcg cagctcaaag
     6661 atgctcccgc tccgatcctt cattttccta ccgtcctcgg cggcgtcgtg ccggtctaca
     6721 acgttcccgg cgtgacgcaa ccgctgcgtt tcagcggtcc ggttctcgcc accatctacc
     6781 tcggcaggat caccaagtgg aaccatcccg ccatcgcaca actcaatccg ggagtcgcgc
     6841 ttccggatac ggatatcgcc gtcgtccatc gctccgacgg ctcgggcacg acctacatct
     6901 tctgtgactt tctctctaag gtctcgccgg acttcaagga aaaggtcggc gtggccacct
     6961 cggtcaactg gccggcaggc gtcggagcga agggcaacga aggcgtctcc ggtctcgtgc
     7021 agcagacgcc cggagccatc ggctacgtcg agctgatcta cgcgctgcag aacaagattc
     7081 cgtacggccc ggtgcagaac aagtccggca agttcatcac ggcgtcgctc gattcggtga
     7141 cggccgcggc ggccggcgtg acgatgcccg acgacttccg tgtctcgatc accgatcccg
     7201 ccgccgctga cgcctatccg atcgcctcat tcacctggat gcttctctat cagcatccgg
     7261 cggacaaagc gcgcagcaaa gcgatggtcg acttcctgaa gtgggctctc gccgacggcc
     7321 agaagttcgc ccccggactc ggctacgcgc cgctgccgca accagttgtc gcgaaggaac
     7381 tgcaggcgct cgcgacggtt caatgaacgc ccaatgaagc gctcctctga cgaccgcagc
     7441 ttccgcatcg gcacgggcgc atttgctgcg ctcgtgttgc tgatcgttct cggcatcgcg
     7501 ttcgagctga cgcggcaatc ggcgctgtc
//
LOCUS       DATLVW010000676         3472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_525225, whole genome shotgun sequence.
ACCESSION   DATLVW010000676 DATLVW010000000
VERSION     DATLVW010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525225"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2092
                     /gene="acnA"
                     /locus_tag="VNN25_19720"
     CDS             <1..2092
                     /gene="acnA"
                     /locus_tag="VNN25_19720"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HXH93818.1"
                     /translation="DLVLTITEMLRKAKVVGKFVEYFGEGAASLGLADRATIANMAPE
                     YGATMGYFPIDEETCRYMTMTGRSDAQVNALRGYFKAQGLFGMPQKGQIDYTTSLELD
                     LSKVGASVAGPKRPQDRIELDNIQSEWMAMLKAAAPAGYGKSESEATRKAKTLVGLRS
                     QDAGNTVTGGGAQDSATAPNALASARKNTSITTEEEMLNNRPTPDRVDADSLTDEFPR
                     AVAELHHGDVLIAAITSCTNTSNPSVMLAAGLLAKKAVEKGLRVPRVVKTSLAPGSRV
                     VTDYYHRSGLQPYLDQLGFYTVGYGCTTCIGNSGPLDPHIEDAIARHDLVGVSVLSGN
                     RNFEARVHQSIRANFLMSPPLVVAFALAGRIDIDMAKEPIGQGSHGEDVYLREIWPTM
                     EEVRTCMAGALDPESFRTLYKNFSDQNPLWNEIPTTGGLLYNWDPASTYIQEPPFFIG
                     EQGMKPGESAAITGARALALFGDSVTTDHISPAGSIKPSSPAGKYLIESGVPVEDFNS
                     YGSRRGNDRVMTRGTFANVRIKNLMVPGVEGGVTVHQPDGEQMSIYDAAMQYQKEGTP
                     LIVIAGQEYGTGSSRDWAAKGTKLLGVRAVVTQSFERIHRSNLVGMGVLPLQFDEGTN
                     AHSLKLDGTEVFDIEVSGDLRPRQKATLVVHRANGSTEKVPVIVRIDTPIEVDYYKSG
                     GILPFVLRQLMGAA"
     gene            2173..>3472
                     /locus_tag="VNN25_19725"
     CDS             2173..>3472
                     /locus_tag="VNN25_19725"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HXH93819.1"
                     /translation="MDFLRSALFRAALASALVTLPAAAAPGAFTLTGTPACSSFPPAI
                     VFQWTPSSGATSYELIRDDGQHTDMSVSPLSKYNYFYYYGDLVAGGPARSYFARASDG
                     TATTDSNTITVAPPTPSCIPIPDPITITGKAFCYPGNPQRVMGPGVELDWHAARLASS
                     YDVYRDGAFLSTVRGDADLYTYYAALESGGFTTTYYVVAKNSAGASTSNTVEINIPAD
                     ICVTEPPVPLLSGSATCNAISQVPSVALNWTPVGIPIAWQLFRDGAPYAVPHGIAYSD
                     TNVVPGHTYRYNVATNALSAPLSNAVTVTVPNTICAPGVQSPDLTALDIKPSAMSGRV
                     GDTIALNVELANLGNLTAAAATARVRLGRGPSMSSADLVLGTITLPALGSGEDIRRTM
                     NVKLPPVAAGTYYLFLSLDEEHVSGEVHFDDDVKASGAFSL"
BASE COUNT          676 a         1125 c         1067 g          604 t
ORIGIN      
        1 cgatctcgtg ctcacgatca cggagatgct ccgcaaagca aaggtcgtcg gcaagttcgt
       61 cgagtacttc ggcgaagggg cagcatccct cgggctcgcg gatcgcgcca cgatcgcgaa
      121 catggccccg gagtacggcg cgacgatggg ctacttcccg atcgacgaag agacctgccg
      181 ctacatgacg atgaccgggc gcagcgacgc gcaggtcaac gcgttgcgcg gatacttcaa
      241 agcgcagggt ctgttcggca tgccgcaaaa ggggcagatc gattacacga cctcgctcga
      301 gctcgatctc tcgaaggtcg gcgccagcgt agccggcccg aaacgtccgc aggaccgcat
      361 cgagctggac aacattcaga gcgagtggat ggcgatgctc aaagccgccg cccccgcggg
      421 ctatggcaag tcggagagcg aagccacgcg gaaggcgaag acgctcgtcg gtctgcgctc
      481 gcaggatgcg ggaaacacgg tcaccggcgg aggcgctcag gattcggcga cggcaccgaa
      541 cgcgctggcg tccgcgcgca agaacacgag catcacgacg gaagaagaga tgctgaacaa
      601 ccggccgacg cccgaccgcg tcgatgccga ttcgctgact gacgagttcc cgcgtgcggt
      661 cgccgagctg catcacggcg acgttctcat cgccgcgatc acttcctgca ccaacacctc
      721 caatccctcg gtgatgctcg cggccggact gctggcgaag aaagcggtcg agaagggact
      781 gcgcgttccg cgcgtggtca agacgtcgct cgcaccaggc tcgcgcgtcg tcactgacta
      841 ctaccatcgc agcggcctgc agccgtatct cgatcaactc ggcttctaca ccgtgggcta
      901 cggatgcacg acctgcatcg gcaactccgg tccgctcgat ccgcacatcg aagacgccat
      961 tgcgcgacac gacctcgtcg gcgtcagcgt cctgtccggc aaccgcaact tcgaagcgcg
     1021 cgtgcatcaa tcgatccgcg cgaatttcct gatgtcgccg ccgctggtgg tcgccttcgc
     1081 gctggccggc cgcatcgata tcgacatggc caaggagccg atcggccagg gatcgcatgg
     1141 cgaggacgtc tatctgcgcg agatctggcc gacgatggag gaagtccgta cgtgcatggc
     1201 cggcgcgctc gatccggagt cgttccgcac gctttacaag aatttctccg atcagaaccc
     1261 gctttggaat gagatcccga cgacaggcgg actgctctac aactgggacc cggcgtcgac
     1321 gtacatccag gagccgccgt tcttcatcgg cgagcagggg atgaagccgg gcgaaagcgc
     1381 tgcgatcacc ggcgcgcgcg cgctggcgct cttcggcgat tccgtcacga ccgatcacat
     1441 cagcccggcc ggatcgatca agccaagctc gccggcaggc aagtacctga ttgagtcggg
     1501 cgttccggtc gaagacttca acagctatgg ctcgcggcgc ggcaacgacc gcgtgatgac
     1561 gcgcgggacg ttcgcgaacg tgcgcatcaa gaacctgatg gtgccgggcg tcgaaggcgg
     1621 cgtcaccgtc catcagccgg acggcgagca gatgtcgatc tacgacgcgg cgatgcaata
     1681 tcagaaggaa ggcacgccgc tgatcgtgat cgcggggcag gagtacggca ccggctcgtc
     1741 gcgcgattgg gccgcgaagg gcacgaagct cctcggcgtc cgtgccgtcg tcacgcaaag
     1801 cttcgagcgg atccatcgct ccaatctcgt cgggatggga gtgctgccgc tgcaattcga
     1861 cgaagggaca aacgcgcact cgctcaaact cgacggaacg gaagtcttcg acatcgaggt
     1921 gtccggcgat cttcgtccgc gacagaaggc aacgcttgtc gtgcatcgcg caaacggcag
     1981 tacggagaag gttccggtaa tcgtccgcat cgatacgccg atcgaagtcg actactacaa
     2041 gagcggcggg atcctgccgt tcgtgctgcg gcagcttatg ggtgccgcgt agaaagaggc
     2101 catccgcaga tgacgcagat ggtcgcagat ggaatcgttc tccatgcgct agagtaccgg
     2161 tcggtccgtc ccgtggattt cctcagatct gctctattcc gtgccgcgct cgcctctgcg
     2221 ctcgtcaccc ttcccgctgc cgcggcgccg ggtgcattca cgctgaccgg tacacccgcg
     2281 tgttcctcat tcccgccggc gatagtcttc cagtggacgc cgtcgagcgg cgcgacgtcc
     2341 tacgagctca ttcgcgacga cgggcagcat acggacatgt cggtatcgcc gctctccaag
     2401 tacaactact tctactacta cggggacctc gttgccggcg gcccggcgcg atcctatttc
     2461 gcgcgggcca gcgacggtac ggctacgacc gactcgaaca ccataacggt cgcgccaccc
     2521 acaccttcat gtataccgat acctgatcca atcacgatca cgggcaaggc gttctgctac
     2581 cccggcaatc cgcaacgcgt catgggcccg ggggtggaat tggactggca tgcagcgcgg
     2641 cttgccagca gctacgacgt ttaccgggat ggtgcctttc tctcgaccgt ccgcggtgac
     2701 gcggatcttt atacctacta tgcggctttg gaatccgggg gattcacgac tacgtactac
     2761 gtcgtcgcaa agaattccgc cggcgcctct acgtcgaaca ccgtggagat caacattcct
     2821 gctgacatct gcgtcaccga gccaccagtg cctttgctct cagggagcgc cacctgcaac
     2881 gccatttcgc aggtgccaag cgttgccctc aactggacgc cggtaggtat tccaatcgcg
     2941 tggcagctct tcagggatgg agctccgtat gcggttccgc acggcatcgc atattcggac
     3001 acaaacgtcg tacccggtca cacctacaga tacaacgtgg ccacgaacgc cctctcggcg
     3061 ccgctctcca atgcagtcac agtcacagta ccgaacacaa tttgcgcacc tggagtgcag
     3121 tcgcccgatc tgacggcgct cgacatcaag ccttccgcga tgagcggacg tgtgggcgac
     3181 acgattgcgc tgaacgttga actcgcgaac ctcggcaacc ttacggcggc ggcagcgacg
     3241 gcgcgcgttc gcctcggacg aggaccctcg atgtcgtccg ccgatctggt gctgggcacg
     3301 atcacgctgc cggcattggg ctcgggtgag gacatcaggc gcacgatgaa cgtgaagctg
     3361 ccgcccgtcg cggcggggac gtattacctc ttcctctctc ttgacgagga gcacgtttcc
     3421 ggcgaagtgc atttcgacga cgacgtgaag gccagcggcg cattcagcct ca
//
LOCUS       DATLVW010000677         5275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_439478, whole genome shotgun sequence.
ACCESSION   DATLVW010000677 DATLVW010000000
VERSION     DATLVW010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439478"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..1276
                     /locus_tag="VNN25_19730"
     CDS             401..1276
                     /locus_tag="VNN25_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HXH93820.1"
                     /translation="MGCDPRLLSGVKLFELLSEEDRAALAAVVDCIHVTAGHTLFEAG
                     HPGESLFVVRSGEIELFIKDNTGQKIVLMNAIEGGLFGELALLDRGARTATAVALSDS
                     ELLELDREDLLLLFQRHPDSAVSMLAAVTGMMRKADELLRTRVSRNVNEEVEEHLTTL
                     QKVADWIAWFSGSMPFLLMNGIWFVAWIIINTIHVGLPWFDPYPFALLTMIVSLEAIF
                     LSCFVLVSQNRQAEKDHVRSDVEYEINVKAELEVAHLHEKTDRMYEQMLARFAVLEQR
                     SAGVSPAADGRLAQR"
     gene            1476..4025
                     /gene="thrA"
                     /locus_tag="VNN25_19735"
     CDS             1476..4025
                     /gene="thrA"
                     /locus_tag="VNN25_19735"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HXH93821.1"
                     /translation="MKQSRPEVFKFGGVAVGNADAIRVALAHVSRAAPGVAVVVSAMN
                     GITDLLLEAGQAALRRDRARCDDAAAQVEARHCELVAELVASPDRATALNAVITESVR
                     ELRSMTESIAVLGELTPRAQDALVARGERLVAAIFAIAAIEIGIDAVYVDATEVIQTE
                     RRLGTLWPNLLKCERAAKKNVVPLLAARQVVVMPGFIASGPDGSVVTLGRGGSDFSAA
                     ILARSIGARALTLFKEVDGLMTADPKSVPAARVIADLHYREAAELAYYGANVLHPRTM
                     IPLVDRRIPLYVRNSFRETSPGTRIAGDVKPGSYPVKALTAIHRQALLSIEGNGMIGV
                     PGVAGRAFTALSQAGHSVSMISQASSESSICFVVPESEADHAVAALEVAFEPERKLHL
                     IDRIDAEKRVALIAVVGLGMRGTSGIAARTFSAIAAAGVNIVAIAQGSSELNITIAVS
                     ESDATRALLALHSEYQLHKIRPLADTTGREAKLTLLGLGQIGRELARQLIAQQPHLRR
                     DLGIDLKVVAVADRSGIRIDDKGFTPAALQRFAKRKESGARLFERGSPLTLRELETMM
                     RDELWMLPSYRPVLVDLTSEETGPLLQEALEYGFHVVCANKKPLAGPQLEFDRLMETA
                     KERELSIRYEATAGAGLPVLDTIAKLQEAGDKIEMILGCFSGTLGYLMTALEDGVAFS
                     EAVRKAWELGYTEPDPRDDLSGMDVARKALILARTLGYRAEPDEIKLEPLVRSDGSAV
                     DAKTFIAGLVSLDDEYRERVDRARRGGKVLRYVATIRKRSISVGVEAVPNTSPLAHLR
                     GTANQVAIYSKRYKTNPLVVSGPGAGATVTAAGVLNDIVAIAMEERRRRSR"
     gene            4022..4936
                     /locus_tag="VNN25_19740"
     CDS             4022..4936
                     /locus_tag="VNN25_19740"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXH93822.1"
                     /translation="MSATAFAPGSIGNVGPGFDVLGLAIDGIGDRVTVALTDGDARVD
                     NITGVDAALVPLDPQRNAAAIAAVAWLRAHGDSRNPIVTIDKGLPLSGGLGGSAASSV
                     AGAFAAALAMGSETSPIELAAAALQGEMTVAGEHLDNIAPAILGGLALSRSVRPIDII
                     ALKVAADWRLALVTPDVRIQTKAAREVLPSTSGRAVWVQQMANTAGVVHAFATGDGEL
                     LRRALDDRYAEPRRAALIPHFNAVKRAALDVGAFACTISGSGPTLVAICADDDIAHRV
                     TAAMQTAFEQVRSIARVSPIARQGVRAV"
     gene            4933..>5275
                     /locus_tag="VNN25_19745"
     CDS             4933..>5275
                     /locus_tag="VNN25_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93823.1"
                     /translation="MSEWLQCIGCERRYDVMEIRYTCDCGDLLNVERDSFPDRDLFEA
                     RRTSRRTIDQSGVWRFREAVLSINEDEIITHPEGATRLYQRESGVEGGNVGRTLQSDS
                     LRAIVGLESPTY"
BASE COUNT          965 a         1713 c         1671 g          926 t
ORIGIN      
        1 aggacgccgt gatcgagagc cgccggaatc acatcgtcgt tcgcgatgtg gtggggctga
       61 aggcgaagtc gtgcgagtgc cacgccgcga tcaatggccg gttgagtgtg ttctcgcaga
      121 aactggggaa gagatcggtg gtttgaatgg atcagagaca agagatcaga gacaagagat
      181 cagagacaag agacaagaga caagagatca gagacaagag acaagagaca agagacaaga
      241 gacaagagac aagagacaag ggtaaggaat ggtctgtctg gtctttgatc cttgatagtc
      301 cctgatctct ggttttcttg tccctggcga tcgctgactt cctgatctct gatctctgat
      361 ctctgatctc tgatctctgc cgcaaagcta caatgccctt atgggatgtg atccgcggct
      421 actctcgggc gtcaagctct tcgaactgct gagcgaggaa gatcgcgctg cgcttgccgc
      481 ggtcgtcgac tgcattcacg tcacggccgg ccatacactc ttcgaagccg gccatcccgg
      541 cgagtcgctc ttcgtcgtcc gcagcggcga gatcgagctc ttcatcaagg acaacaccgg
      601 ccagaagatc gtattgatga acgcgatcga gggcggtctc ttcggcgagc tcgctcttct
      661 cgaccgtggt gcgcggacgg ccaccgcggt cgcgttatcg gacagcgagc tgctcgagct
      721 cgaccgggag gatctcctcc ttctcttcca acgccatccc gacagcgcgg tgagcatgct
      781 cgcggcggtc accgggatga tgcgcaaagc tgacgagctg ctgcgcaccc gcgtgtcacg
      841 caacgtcaac gaagaggtgg aggaacatct caccacgctg cagaaggtgg ccgactggat
      901 cgcctggttc agcggcagca tgcccttcct gctcatgaac ggcatctggt tcgtggcgtg
      961 gatcatcatc aacacgatcc atgtaggact gccatggttc gatccgtacc cgttcgccct
     1021 cctcacgatg atcgtctcgc tcgaagcgat cttcctctcc tgcttcgtcc tcgtcagcca
     1081 gaaccgtcag gccgagaagg accacgtgcg gtccgacgtc gagtacgaaa tcaacgtgaa
     1141 ggcggagctg gaagtagcgc acctgcacga gaagacggac cgcatgtacg agcagatgct
     1201 ggcccgcttc gcagtgctcg agcaaaggag tgcaggcgtc tcgcctgcgg ccgatgggcg
     1261 tctcgcccag cggtaatgcg cgcaaacggg ctgctcgaac cgcatcgttt attgaacgca
     1321 gggtgtgcgg acgtctcgcc cagaggtaac gcgcgcaaac agaccgctca aagcttgtaa
     1381 ccgccccgct tattgaacgc tccagacggc gaggcgagac gcctcaccgc cgcgagcgag
     1441 acgctcgcac tcctcccgct atactccgcg tcctcatgaa gcagtctcgt ccggaggtct
     1501 tcaagtttgg cggtgtcgcc gtcggcaatg cggatgcgat ccgcgtcgcg ctcgcgcacg
     1561 tgagccgcgc tgcgccgggc gttgccgtcg tcgtttcggc gatgaacggc atcaccgatc
     1621 tgctccttga agccggacag gcagcgctcc gccgcgaccg cgcgcgttgc gatgacgccg
     1681 ccgcgcaggt cgaggcgaga cactgcgagc tggtcgcgga actggttgcg tcgcccgatc
     1741 gcgcgactgc cttgaacgcc gtcatcaccg aatccgtccg cgagcttcgc tccatgacgg
     1801 agagcatcgc cgtcctcggc gagctgaccc cgcgcgcgca ggacgcgctg gtagcgcgcg
     1861 gcgagaggct cgtcgcggcg atcttcgcga ttgcagcaat cgagatcgga atcgatgcgg
     1921 tttatgtcga tgcgaccgag gtgattcaga cggagcggag gttgggaacg ctctggccga
     1981 atctgctgaa gtgtgagcgc gcggcgaaga agaatgtggt gccgctgctc gccgcgagac
     2041 aagtcgtcgt gatgcctggc ttcattgcca gcgggccgga tggctcggtc gtcaccctcg
     2101 ggcgcggcgg ctccgacttt tccgcagcga tcctggcgcg cagcatcggc gcgcgcgcgc
     2161 tgacgctctt caaggaagtc gacggcctta tgaccgccga tccgaagagt gttcccgccg
     2221 cgcgcgtcat cgccgacctt cattaccgcg aagccgccga gttggcctac tacggcgcga
     2281 acgttctgca tccgcgcacg atgatcccgc tcgtcgaccg gcgcatcccg ctctacgtgc
     2341 gcaacagttt tcgcgagacc tcgcccggaa cgcgcatcgc cggcgacgtg aagcccggct
     2401 cctatccggt gaaagcgctg accgccattc atcgccaggc ccttctatcg atcgaaggaa
     2461 acggaatgat cggcgttccc ggcgttgccg gacgcgcgtt caccgcgctc tcgcaggcag
     2521 gccattccgt ctcgatgatc agccaggcct cgagcgagtc gagcatctgc ttcgtcgtcc
     2581 ccgaatcgga agcggatcac gcggtggccg cgctcgaagt ggcgttcgag ccggagcgca
     2641 aacttcatct catcgaccgc atcgatgcag agaagcgcgt cgccctcatc gccgtcgtcg
     2701 gactaggcat gcgcggcacc tcgggcatcg ccgcgcggac cttctccgcg atcgctgctg
     2761 ccggcgtgaa catcgtggcg atcgcgcagg gctcgtcgga gctcaacatc acgattgccg
     2821 tctccgaaag cgatgccacc cgcgcgctgc ttgcgctcca ttccgaatat cagctccaca
     2881 agatccggcc gctcgccgac acgaccggcc gtgaggccaa gctcaccctt ctcggcctcg
     2941 ggcagatcgg acgcgagctg gcccgccagc tcatcgctca gcaaccgcac cttcgccgcg
     3001 acctcggcat cgatctcaaa gtcgtcgccg tcgccgaccg ctccggcatc cgcatcgacg
     3061 acaagggatt tactcccgcg gcgctgcaac gattcgcgaa acgcaaagag tccggcgcgc
     3121 gtcttttcga gcgcggctcg ccgctgacgc tgcgcgagct cgagacgatg atgcgcgacg
     3181 agctgtggat gctgccgtcc taccggccgg tgcttgtcga tctcacctcg gaagagaccg
     3241 gtccgctgct gcaggaagcg ctggagtatg gattccacgt cgtctgcgcg aataagaaac
     3301 cgctggccgg accgcagctc gaattcgacc ggctgatgga gaccgcgaaa gagcgtgagc
     3361 tatccattcg ttacgaagcc acggccggag cggggttgcc ggtgctcgac acgatcgcga
     3421 aactgcagga agcgggcgac aagatcgaaa tgattctcgg ctgcttttcc ggaaccctcg
     3481 gctatctgat gaccgcactg gaggatggtg ttgcgttctc cgaggcggtg cgcaaagcgt
     3541 gggagctcgg atacacggag cccgatccgc gcgacgatct ctcgggcatg gatgtcgcgc
     3601 gcaaggcgct gatcctggcg cgcacgctcg gataccgcgc ggagccggac gagatcaaac
     3661 tcgagccgct cgttcgcagc gacggcagtg cggtcgacgc gaagacgttc atcgctggtc
     3721 tcgtgtcgct cgatgacgag tatcgcgagc gtgtcgatcg cgcgcgccgc ggcggcaagg
     3781 tccttcgcta cgtggccacc atccgcaagc gttcgatctc cgtcggcgtc gaggcagttc
     3841 cgaatacttc gccgctcgcg catctgcgcg gaaccgctaa ccaggtggcg atctactcga
     3901 agcgctacaa aacgaatccg ctcgtcgtct ccggccccgg cgccggcgca accgtgaccg
     3961 cggccggcgt tctcaacgac atcgtggcga tcgccatgga ggaacggcgg aggcggagcc
     4021 gatgagtgcg acagcgttcg cgcccggatc catcggcaac gtcggccccg gcttcgacgt
     4081 cctagggctg gcaatcgatg gaatcggcga ccgcgtgact gttgcgttga ctgatggtga
     4141 tgcacgcgtc gacaacatca ccggcgtcga cgccgcgctc gtcccgctcg atccgcaacg
     4201 caacgcggcc gcaattgccg ccgtcgcgtg gcttcgcgcg cacggcgatt cgcgcaatcc
     4261 gatcgtgacc atcgacaaag gtctcccgct gtcgggcggc ctcggaggca gtgcagcgag
     4321 cagcgtcgcc ggtgcgttcg ctgccgctct ggcgatgggg agcgagacat cgccgatcga
     4381 gctcgccgcg gcagcgttgc aaggcgagat gacggtcgcc ggcgagcacc tcgacaacat
     4441 cgccccagcc atcctcggcg gactggcact ctcgcgctcc gtccggccga tcgacatcat
     4501 cgcgctgaaa gttgccgccg attggcgtct cgccctcgtg acacccgacg tccgcatcca
     4561 gacgaaagca gcgcgcgaag tgttgccgtc gacctcaggg cgtgcggttt gggtccagca
     4621 gatggcgaac accgccggcg tcgtccacgc gttcgcgacc ggtgacggcg agttgctgcg
     4681 ccgcgcgctc gacgaccgct acgccgaacc gcgccgcgcc gcgctcatcc cgcatttcaa
     4741 cgcggtaaag cgcgccgctc tcgacgtcgg cgcgttcgcc tgcacgatca gcggatccgg
     4801 tccgaccctc gtcgccatct gcgccgatga cgacatcgca catcgcgtga ctgccgcgat
     4861 gcagacggcc ttcgagcagg tccggtcgat tgcccgcgtc tcaccgatcg cgcgccaagg
     4921 agtgcgtgcc gtatgagcga atggctgcag tgcatcggat gcgaacgccg ctacgacgtg
     4981 atggagatcc gctacacatg cgactgcggc gatctgctga acgtggagcg cgactcgttt
     5041 cccgatcgcg acctcttcga agcgcgccgc accagccgcc gtacgatcga tcaaagtggc
     5101 gtatggcgat tccgcgaagc ggttttgtcg atcaatgaag acgagatcat cacgcacccc
     5161 gaaggcgcaa cgcggttgta tcagcgggag tcgggagtcg agggaggaaa cgtaggtcgg
     5221 actctccagt ccgactcttt gcgcgcaatc gtcggactag agagtccgac ctacg
//
LOCUS       DATLVW010000678         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_481952, whole genome shotgun sequence.
ACCESSION   DATLVW010000678 DATLVW010000000
VERSION     DATLVW010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481952"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VNN25_19750"
     CDS             complement(<1..215)
                     /locus_tag="VNN25_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH93824.1"
                     /translation="MTTSLLHHDYTFADYLALEHDSEVKHEFDAGEILAMFGGTARHC
                     ALAAKIVTAMGTTRAPGCTVFTSDMRVR"
     gene            375..2069
                     /locus_tag="VNN25_19755"
     CDS             375..2069
                     /locus_tag="VNN25_19755"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXH93825.1"
                     /translation="MRRFQTALAAGLLAGVTDALLVHRPVGLGLARALFFLPLVWIVW
                     CLLVRALFLLRPLERWADAAVVAAGPGLLLTSRIYPALRNVEVPSVASGAICVATAAV
                     LIVLAHFAPARAIAFGAMLAIAWLAGTALFVLCSGYERAAPATVSAPAGAPNVLLIFL
                     DTVRYDDAQQMPNLRKFASTGVVYDRAWASAPWTLPSHLGVLTGMPPWKVPYDSASHR
                     FLYDGPTLAERFAARGYTTSAIFANPLLGLDPLFHRGFQHFQVSARCVPCLSGLATLL
                     SRALERAGWNPTFFAPPEWMKASDVTAKALRTTERTDGPYFLALNYMDAHSPYYVERA
                     CRGRGFEQATDADWKAWGRTYNSRAPLAPEPAARLRAQYRAAMQCMDLSIGRLLDEVQ
                     RMRNGRQTVIAIVGDHGEEFGSHDMVGHGQSLYRQVLNVPVIVKAPDQAPQRVATEIS
                     TLDLYDQVLRYLPESRQPLHPHSWPVIASYKVIDSPGPAKLTAAFSAVQGRYHLIQWR
                     DGSEELYDLDSDPDESRPLPIRADDRAFAELAAELRRNTAEENAAVISLHGVGYLQ"
     gene            complement(2154..2230)
                     /locus_tag="VNN25_19760"
     tRNA            complement(2154..2230)
                     /locus_tag="VNN25_19760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2194..2196),aa:Arg,seq:ccg)
     gene            complement(2419..>3609)
                     /locus_tag="VNN25_19765"
     CDS             complement(2419..>3609)
                     /locus_tag="VNN25_19765"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH93826.1"
                     /translation="MAENGLRSVRLSGGGDPLFHREINDVLTHLASRNIVVDNVTTNG
                     VALNRDIASFLVANKGREVIFSLNAVDAADYARMMKVKPALFDKVIENIKTLVEVRGD
                     SIYPSIVTQFLIDRDNVTRMVEMYDLARSLGVDRIAVNAVLEIPRERIDRELLLRPED
                     ADLAEPFVEELLRKDADAGLLQIDFAVWGWNKMMKRVRDRVGIQPTNMYPVAPSFKDR
                     NDGCFFAWYTAAITGNGDIRPCCLLLNPDVPPLGNVHEASITEHWRGPMFEKMRDEMR
                     EILLRGGKVDYDPKRFQVLKEVCVKPGLCWLKNMYFKFDEEFYAELDAKLEVVRKKEV
                     RWIGTPKQIKRRADVFLYDHPKVRELIAGQRWRRTYDWLRDSSRPLRVALKRRLHINL
                     TDAA"
BASE COUNT          612 a         1102 c         1180 g          715 t
ORIGIN      
        1 cgaactcgca tatcggatgt gaagacggtg cagccgggcg cgcgggtagt tcccattgcg
       61 gtgacgatct tcgctgccag cgcacagtgc cgcgccgttc caccgaacat cgccaggatc
      121 tcgcctgcat caaattcgtg cttgacctca ctgtcgtgct cgagcgcgag gtagtccgcg
      181 aacgtgtaat cgtggtgcaa cagagaggtt gtcatcaggg tctaggatag cgcgcgctgc
      241 aggcatcgcg gtccttcagc taatgcttcc gttgcgtttt acgcccggcg agtacgagcg
      301 tagatgtggc ggttaacgac tacttgaaat cgatggtggg cctttgggag tagttgcgaa
      361 atatgattgc gcgcgtgcgc cggttccaga ctgcactcgc cgcagggctc ctggccggag
      421 taactgacgc gctgctcgtt catcgtccgg tggggctggg gctcgcgagg gcgctcttct
      481 tccttccttt ggtctggatc gtctggtgtc tgctggttcg ggcgctattc cttctccggc
      541 cgctcgagcg gtgggcggat gccgccgtcg tcgccgcagg tccgggactt ctgctgacca
      601 gccgcattta cccggctctg cgcaacgtcg aggtgccgtc agttgcgagc ggcgccatct
      661 gcgttgccac cgccgcggta ctgatcgtat tggcgcactt cgcacctgcg cgagcgattg
      721 ctttcggcgc aatgctggcc atcgcgtggc tcgccgggac cgcgctcttc gtgctgtgca
      781 gcggatacga gcgcgcggcg ccggctaccg tttccgcgcc cgccggcgca cccaacgtgc
      841 tgctcatttt tctcgacacc gtccgctacg acgacgcgca gcagatgccg aacctgagga
      901 agttcgcgtc gaccggtgtt gtgtacgacc gcgcgtgggc ttccgcgccg tggacgctgc
      961 cgtcgcactt aggcgtcctt accggtatgc cgccgtggaa ggtgccgtac gactccgcgt
     1021 cgcaccggtt tctctacgac ggtccgacgc tcgcggagcg gttcgcggca cgcggctaca
     1081 ccacctcggc aattttcgcc aatcctttgc tcgggctgga ccctctcttt caccgcgggt
     1141 tccagcactt ccaggtctcg gcgcgctgtg tcccgtgtct ctcaggcttg gcgacgctgc
     1201 tgtctcgagc gctggaacgt gcaggatgga atccgacgtt cttcgctccg cccgaatgga
     1261 tgaaagcatc cgacgtgacg gcgaaggcgc tgcgcactac cgagcgcacg gacggaccgt
     1321 atttcctcgc gctcaactac atggatgcgc actcgcctta ctacgtcgag cgcgcctgcc
     1381 gcggccgcgg attcgagcaa gcgaccgacg ccgattggaa ggcgtggggg aggacctaca
     1441 attcgagagc gccgctcgcc cccgagccgg ctgcgcgtct gcgcgcgcaa taccgggctg
     1501 cgatgcagtg catggacctc tcgatcggca ggctgctcga cgaggtccag cgaatgcgga
     1561 atggacggca gaccgtcatc gccatcgtcg gagatcacgg cgaagaattc ggcagccacg
     1621 acatggtcgg gcacggtcaa tcgctctacc ggcaagtcct gaacgttccg gtgatcgtaa
     1681 aagcgccgga ccaggcgccg caacgagtcg ccacggaaat ctcgacgctc gacctctacg
     1741 atcaggtcct ccgctatttg ccggagtctc gtcagcctct ccatccacat tcgtggccgg
     1801 tgatcgcctc ctataaagtg atcgactctc ctgggccggc gaaattgacg gcggcattta
     1861 gcgccgttca ggggaggtac cacctgattc agtggcgcga cgggagcgaa gagctttacg
     1921 accttgattc cgacccagac gagagcaggc cgctgccgat ccgcgcagat gatcgggcgt
     1981 tcgccgaatt ggccgccgag cttcgccgca acacggcgga ggagaacgcc gccgtcatca
     2041 gcctgcacgg cgtcgggtat ctgcaatagc tttgagagcg gaagccgggc agccgccaga
     2101 acgccgaatg tgtggtgttc ctgacggctc gttccgcgag aagaaaatga agttggtgcg
     2161 ccctgcagga ttcgaacctg cgacctacag ctccggaggc tgtcgctcta tccagctgag
     2221 ctaagggcgc gcacgtggaa gcgtggcgga ttgtatggtg cgcaacgtcg gcgggcaacg
     2281 tgcgatttca acgaggtcct gaacgtaggt cctgaacgta ggtgttgaac gtaggtcgga
     2341 ctctccagtc cgactttcct cccgatgccg aagaaaaccg tcggactgga gagtccgacc
     2401 tacgtcaggc gcgatcgttc atgcggcgtc ggtgaggttg atgtgcaggc ggcgcttcag
     2461 cgccacgcgt agggggcggg aggagtcgcg gagccagtcg tacgttcggc gccagcgctg
     2521 gccggcgatc agttcccgaa ccttgggatg atcgtagagg aagacgtcgg cgcggcgttt
     2581 gatctgcttc ggtgttccga tccaccgcac ttccttcttg cgcacgacct ccagcttcgc
     2641 atccagctcg gcgtagaact cctcgtcgaa cttgaagtac atgttcttca gccagcagag
     2701 gcccggcttg acgcagacct ctttcaggac ctggaagcgt ttcgggtcgt agtcgacctt
     2761 gccaccgcgg agcaggatct cgcgcatctc gtcgcgcatc ttctcgaaca tcggaccgcg
     2821 ccaatgctcg gtgatcgacg cctcgtgaac gttgccgagg ggtgggacgt cgggattgag
     2881 gaggagacag caggggcgga tgtcgccgtt gccggtgatg gcggcggtgt accaggcgaa
     2941 gaagcagccg tcgttgcggt ctttgaacga cggcgcgacc ggatacatat tcgtcggctg
     3001 aatccctacg cggtcgcgaa cgcgcttcat catcttgttc cagccccaga cggcgaagtc
     3061 gatctgcagg agtccggcgt cggcgtcttt gcgcagcagc tcttccacga acggctcggc
     3121 aagatcggca tcctcgggac gaagaagcag ctcgcggtcg atgcgttcgc gcgggatctc
     3181 gagcacggcg ttcacggcga tgcggtcgac gccgagggat cgggccagat cgtacatctc
     3241 gaccatacgc gtgacgttgt cgcggtcgat gaggaactgc gtcacgatcg agggatagat
     3301 cgaatcgccg cgcacttcca cgagagtctt gatgttctcg atcaccttgt cgaacagcgc
     3361 cggcttcacc ttcatcatcc gggcgtagtc ggcggcatcg acggcgttga gcgagaagat
     3421 tacctcgcgc cctttgttgg cgacgaggaa gctggcgatg tcgcggttga gggcgacgcc
     3481 gttcgtggtc acgttgtcga cgacgatgtt gcgcgaggcg aggtgggtga ggacgtcgtt
     3541 gatctcgcgg tgaaagagcg gatcgccgcc gccggatagg cgaacggagc gcaggccgtt
     3601 ctccgccat
//
LOCUS       DATLVW010000679         6439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_238278, whole genome shotgun sequence.
ACCESSION   DATLVW010000679 DATLVW010000000
VERSION     DATLVW010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238278"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VNN25_19770"
     CDS             <1..839
                     /locus_tag="VNN25_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93827.1"
                     /translation="HHLLPEAYLFGFASMVSSSLLRSSYLDGRYSNTGFPDYFLKTFF
                     WKTPLPIIAALVVGLLLAWRRRKEGLGVLAIPIAVYVLYAVTAHINIGHRHLFPIFPF
                     LYVLCGAAGVWWSSLRKRRALVGIVAAAWLVLGAVVVVLPRPALVINQHLAYINEFGG
                     GPRAGAMKLSDSNFDWGQDLKRLGQWYSASGIREPLNLVYFGTADPRSYGIRYRNLRT
                     PDFPEPQGPGWFAISQTDYIGLEFDPNHRSDYWQSMLARHGAHRVETPAHSIFVYRLT
                     GP"
     gene            924..1781
                     /locus_tag="VNN25_19775"
     CDS             924..1781
                     /locus_tag="VNN25_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXH93828.1"
                     /translation="MSIRPLPPDDEQEKLELEAVEVHLLTEGIYQHYGFDFRDYSLPS
                     LRRRLWKRIYAEGLVTISGLQEKVLHDPSCMERLLLDLSINTTAMFRDPSFYLAFRQE
                     IVPLLRTYPFVRIWHAGCSTGEEVYSMAILLQEEGLLERSRIYATDINEAVLQRAKDG
                     IFPLNTMQENTANYIAAGGTGTFSKYYTARYDYSLFRPSLRENVVFAQHNLVTDASFN
                     HFNVIFCRNVLIYFNNHLQERVQELFLNSLEMFGILGLGKKETIRYTNVASNYEELDA
                     EERLYRRIR"
     gene            1924..2586
                     /locus_tag="VNN25_19780"
     CDS             1924..2586
                     /locus_tag="VNN25_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015139342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXH93829.1"
                     /translation="MNRRQMWSPVIERPPNRVTTLPGHRSPELIVIGCSLGGMHALQM
                     ILSNLSRGFCVPIVVAQHRHKKSNEGLPAYFRRKTDLKVVDAEDKQWIEPGHVYLAPA
                     DYHLLIERNGARGELSLSVDERVHYSRPSIDVLFESAADAYGDRLIGIVLTGSNDDGA
                     QGAARIKARGGIVVAEDPATAEAPAMPRAAIAASHVDQILRLEQIAPFLVEVCQTSVS
                     HS"
     gene            2583..4169
                     /locus_tag="VNN25_19785"
     CDS             2583..4169
                     /locus_tag="VNN25_19785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93830.1"
                     /translation="MTPFPDEKVNILLVDDQPNNLLALESILGNEQNLVRAESGRAAL
                     RALLDAEFAVILLDVQMPDLDGFETASLIRERDKSRDTPIIFLTALSRSETHVFRGYE
                     LGAVDYIFKPFEPEILRSKVNVFVELFRKTQVLTRQAHELARLSKQNELILDAAAEGV
                     LGVDLEGVATLVNPAAARMMGRRAEEVRGRQVHSLLHPAFPGVSTCHVERCPLNAALH
                     GGQMRDEVADTFFRDDGTSFPVEFCASMMYDENNQAVGSVVTFRDVSEKRAAALAAEN
                     ERRYREAESQNRAKDNFLATLSHELRTPMTSILGWVQFLRTGEFSNDELREALHMIEN
                     SARLQKRLIDDMLDVSRIILGKFQVDLRPTHLSEIVEAAVTNARPDATERGVRLTLDL
                     IRPPDDVVDADAVRLQQVIGNILSNAIKFTPSGKQVDLRLDRVDHKLQISVSDEGEGI
                     EPSFLPHVFERLRQADASAKRSGLGLGLAIARHIIDLHHGDIRAESEGLGKGARFTVT
                     LPVVEHARMGGGRDEDVVRA"
     gene            complement(4288..4914)
                     /locus_tag="VNN25_19790"
     CDS             complement(4288..4914)
                     /locus_tag="VNN25_19790"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HXH93831.1"
                     /translation="MANDPCLDLIFRNAHTHMAWLDKPVSDATLHELYDTMKWGPTSA
                     NTNPARFLFIRSKAAKERLKLSLAPGNVEKTMAAPVTVVVAYDLKFYEKLPKLFPHAP
                     GMRDNFASNPQLIEMTARRNSSLQGAYLIIAARGLGLDCGPMSGFDNARLDHDFFEAG
                     RECQGCDQEFFPEGHVKSNFLINLGYGDASKVQPRNPRLTFEEACTLL"
     gene            5215..>6439
                     /locus_tag="VNN25_19795"
     CDS             5215..>6439
                     /locus_tag="VNN25_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH93832.1"
                     /translation="MSTKDGSGYPIGKIIFASGLGTMIEWYDFYIFGSLAVVMSQLMF
                     PKGNPAWALIQTWALFATGFVVRPFGAIVFGRVGDLIGRKYAFLVTLTIMGLSTFVIG
                     LLPTYASVGLLAPSILLLLRLLQGLALGGEYGGAAVYVAEHVPDNKRGYYTSFIQVTA
                     TLGLFVSLGVILGVKRMMSDADFKAWGWRLPFLISIVLVGISLWIRMTLKESPLFASI
                     KAQGKSSIAPLRESFGNWKNLRIVLLVLFGATAGQGIVWYTGQFYALSFLQTVLKVDL
                     AKASIIVAAALLLAMPFFVVFGALSDKIGRLKIMMAGNLLAAITYLPIYHAMHDHVGD
                     PKNPNMAILIGLVFIQVIYVTMVYGPIAAFLIESFPAKIRYTSFSLPYHLGNGWFGGT
                     LPLIAGLLVERTHDIF"
BASE COUNT         1192 a         2126 c         1868 g         1253 t
ORIGIN      
        1 accaccacct gttgccggag gcgtacctgt tcggtttcgc ctcgatggtc tccagctcgc
       61 tgctgcgctc gtcgtacctc gatggccgct actcgaacac cggcttcccc gactacttcc
      121 tcaagacatt cttctggaag acaccgctgc ccatcatcgc cgccctggtc gtcgggctgc
      181 tactggcatg gcggcggcga aaggagggtc tcggcgtcct ggccatcccc atagcggtct
      241 acgttctgta cgcagtcacc gcgcacatca atatcggcca ccgccatctc tttcccatct
      301 ttcccttcct ctatgtgctt tgcggcgcgg cgggtgtgtg gtggtcatcg ctgcggaagc
      361 gccgtgcgct agtcggcatc gtcgcggccg catggctcgt gctcggcgcg gtcgtggtcg
      421 tcctcccgcg gccagcgttg gtcatcaacc agcatctcgc ctacatcaac gaattcggcg
      481 gagggccgcg cgcgggtgcg atgaagctct ctgactcgaa tttcgactgg ggtcaggatc
      541 tgaagcgcct cgggcagtgg tactcggcca gcgggatcag ggagcccctc aacctcgtct
      601 acttcggcac cgccgacccg cgcagctacg gcattcgcta tcgcaacttg cgcacccccg
      661 atttccccga gccccagggg cccggatggt ttgcaataag ccagacggac tacatcggcc
      721 tcgagttcga tcccaaccac cgcagtgact actggcagtc gatgctggct cgccacggcg
      781 cgcatcgcgt agagacgccc gcgcactcga tcttcgtcta tcggctcacc ggcccctgaa
      841 cgccgggcgc ccgcgtccgt cacgaccccg gaattgcccc aaatcgtggt gcgcatggaa
      901 tctgcaacct gggaccgtaa ctgatgagta tccgtccgct accgcctgat gatgagcagg
      961 agaaactcga gctcgaagca gtcgaggtgc atctactcac tgaaggcatc taccagcatt
     1021 acggcttcga tttccgcgac tactccctcc cttcgcttcg gcgcaggctc tggaaacgga
     1081 tctacgcgga aggtctggtc acgatctccg gcttgcagga gaaggtgctg cacgatccgt
     1141 cgtgcatgga gcggttgctc cttgatctct ccatcaacac gacggcgatg ttccgcgacc
     1201 cgtcgttcta tctcgccttc cgccaggaga tcgtcccgct gctgcgtacg tatccgttcg
     1261 tgaggatctg gcatgccggc tgttccaccg gcgaagaggt ctactcgatg gccatcctgc
     1321 tgcaggaaga gggactgctc gagcgcagcc gcatctacgc caccgacatc aacgaagcag
     1381 tgttgcagcg cgccaaagac ggcatctttc cgctgaacac gatgcaggag aacaccgcca
     1441 actacatcgc cgccggtggc acggggacat tctcgaagta ctacacggcc cgctacgact
     1501 actcgctctt ccggccgtca ctgcgcgaga acgtggtctt cgcacagcac aacctcgtca
     1561 ccgatgcatc gttcaatcat ttcaacgtca tcttctgccg caacgtcctc atctacttca
     1621 acaatcactt gcaggagcgg gtgcaggagc tctttctcaa cagcctggag atgttcggaa
     1681 tcctcggtct cggcaagaag gagacgatcc gctacacgaa cgtagccagc aactacgaag
     1741 agctcgatgc cgaggagagg ttgtatcgga ggataaggtg agttgcgctt cgcgcgttgt
     1801 cagttgtcgg ttgattgcgg ctccgccgcg tagtggcatc cgacaacgcg cgaagcgcaa
     1861 ccaaccgaca accgacaacg ccgcaggcaa taaaccgaca accgacaacg ccgaaggcaa
     1921 tccatgaacc gccgccagat gtggtctcca gtcatcgagc ggccaccgaa ccgcgtcacc
     1981 acgctacccg gccaccgctc ccccgagctc atcgtcatcg gctgctccct cggcggcatg
     2041 cacgcgttgc agatgattct gagcaacctg agtcgcggct tctgcgtgcc gatcgtggtc
     2101 gcgcagcatc ggcacaagaa atcgaatgaa ggattgccgg cctacttccg ccgcaagacc
     2161 gatctcaaag ttgtcgacgc tgaagacaaa cagtggatcg agcctggcca tgtgtatctg
     2221 gcgccggccg attatcacct cctcatcgag cgcaacggtg ctcgtggaga gctcagtctc
     2281 tcggtcgatg agcgcgttca ctactcccgt ccgtcgatcg acgttctctt cgaatcggct
     2341 gccgatgcct acggcgaccg gctcatcggc atcgtgctca cggggtcgaa cgacgacggc
     2401 gcgcagggcg cggccaggat caaagcccgc ggcggcatcg tcgtcgcgga ggatccagcg
     2461 accgccgagg cgccggcaat gccgcgggcg gcaatcgcgg catcgcacgt ggatcagatc
     2521 ttgcgtttgg aacagatagc gcccttcctg gtcgaggtct gtcaaaccag cgtgagccac
     2581 tcatgacccc gttccctgat gagaaggtca atatcctgct ggtcgacgac cagcccaata
     2641 acctcctcgc cctcgagtcg atcctcggca acgagcaaaa cctcgtgcgc gccgagtcgg
     2701 gccgcgctgc acttcgggcc ctgctcgatg ccgagttcgc cgtcattctg ctcgacgtcc
     2761 agatgcccga cctcgatggc ttcgagacgg ccagtctcat acgcgagcgc gataaatcgc
     2821 gcgacacgcc gatcatcttc ctcactgcac tgagccgcag cgagacgcac gtctttcgcg
     2881 gatacgagct cggtgccgtc gactacattt tcaaaccgtt cgagccggag attctgcggt
     2941 cgaaggtcaa cgtcttcgtc gagctgttcc ggaagacgca ggtgctgacg aggcaggcgc
     3001 acgaactggc gcgtctgagc aagcagaatg aactgatcct cgatgctgcc gcggagggtg
     3061 tcctcggcgt cgatctcgaa ggcgtggcta cgctcgtcaa cccggcggct gcgaggatga
     3121 tgggacgccg cgccgaagag gtcagagggc gccaggtcca ctcgctgctt cacccggcat
     3181 tcccgggcgt ctcgacctgc catgtcgaac gctgcccgct gaacgcggcc cttcacggcg
     3241 gacaaatgcg cgacgaagtg gccgatacgt tctttcgcga cgacgggacc agtttcccgg
     3301 tcgagttctg cgcttccatg atgtacgacg agaacaatca ggccgtcggc tccgtcgtga
     3361 ccttccgcga tgtcagcgag aagcgcgcgg ccgcgctcgc cgccgagaac gagcgccgct
     3421 accgcgaggc cgagtcgcag aaccgcgcca aagacaactt cctggctact ctgtcgcacg
     3481 agctgcgcac gccgatgacg tcgatcctcg gatgggtcca gttccttcgg accggcgagt
     3541 tctcgaacga tgaactgcgc gaggcgctgc acatgatcga gaacagcgcc cgcctgcaga
     3601 agcgcctcat cgacgacatg ctcgacgtct cgcgcatcat tctgggcaag ttccaggtcg
     3661 atctccggcc gacgcatctg tcggagatcg tcgaagcggc ggtcaccaac gcgcgtcccg
     3721 acgcaacgga gcgcggcgtt cgtctgactc tcgacctcat ccgcccgccc gatgacgtgg
     3781 tcgacgccga tgccgtccgc ctgcagcagg tcatcggcaa catcctctcg aacgccatca
     3841 agttcacgcc atcaggaaaa caggtcgatc tgcgcctcga ccgcgtcgat cacaagctcc
     3901 agatcagcgt gagcgacgaa ggggaaggga tcgagccgtc gttcctcccg cacgtcttcg
     3961 aacgcttgcg ccaggccgac gcctcagcaa aacgaagcgg tctcggcctc ggcctcgcca
     4021 tcgcccgcca catcatcgac ctccaccacg gcgacatccg cgcggagtcg gagggattgg
     4081 gcaagggtgc gcgctttacg gtgacgctgc cggtcgtaga gcacgcgcgg atgggcggcg
     4141 gccgcgatga agatgttgtt cgagcctagc cacaagcaca gacaagagtg tctgtgccac
     4201 attgcttcgg gcttttgccg gactcactat cgtttgtgct ttgtgccgat tcagtgtggc
     4261 acagacactc ttgtctgtgc ttcattctca tagcagcgtg caagcctcct cgaacgtcag
     4321 ccgcggattg cgtggctgca ctttcgaggc gtcgccgtaa ccgaggttga tgaggaagtt
     4381 cgatttgaca tggccctcgg ggaagaactc ctgatcgcaa ccctggcact cgcggccggc
     4441 ctcgaagaaa tcgtggtcca gacgcgcatt gtcgaagcct gacatcggcc cgcagtcgag
     4501 cccgaggccg cgcgcggcga tgatgagata ggcgccttgt aatgacgaat tgcgccgcgc
     4561 cgtcatctcg atcaattgcg gattcgaagc gaagttgtcc ctcatccccg gagcgtgcgg
     4621 aaagagcttc ggcagcttct cgtagaactt cagatcgtag gcaacgacga ccgtgaccgg
     4681 cgccgccatc gtcttctcga cgttgccagg cgcgaggctc agcttcagcc gctccttcgc
     4741 cgctttcgac cggatgaaga gaaagcgcgc cggattcgtg ttcgcactcg tcggccccca
     4801 cttcatcgtg tcgtacagct cgtgcagcgt tgcatcactg accggcttgt cgagccaggc
     4861 catgtgcgtg tgcgcattgc ggaaaatgag atcgagacag ggatcgttgg ccatgcactg
     4921 gatgcgtgca ggaagcgcac ccgaggagtg cgggcgtctc gcacgcggcc accaggcgtc
     4981 ccgcccggtg gtggttcacg aacacgaaag acgcatcgag ttatccgatt gggaagatca
     5041 tctttgcctc gggcatccca gcccggtagt gatcgtgcgc ataggcacct cgaagaccgg
     5101 ccactcgaac ttcgccggca accttggcta tctccacacc agcgggcgag gacgccctcg
     5161 cgagcgagac gctcgcactc ctaacgtaca cttcgcacct aaggagagcg acgcatgagc
     5221 acgaaagacg gatcgggtta tccgatcggg aagatcatct ttgcctcggg actcggcacg
     5281 atgatcgagt ggtacgactt ctacatcttc gggagccttg ccgtcgtgat gtcgcagctc
     5341 atgtttccga aaggcaatcc cgcctgggcc ctcattcaaa cctgggcgct cttcgcaacc
     5401 ggcttcgtcg tgcgtccgtt cggcgccatc gtcttcggcc gtgtcggcga tctcatcggc
     5461 cgcaagtatg ccttcctcgt cacgttgacg atcatgggcc tctcgacctt cgtcatcggt
     5521 ctgctgccga cctacgcgtc cgtcggatta ctggcaccct cgatcctgct cctgcttcgc
     5581 ctgctccaag gtctcgccct cggcggcgaa tacggcggcg cagcggtcta cgtcgcggag
     5641 catgttcccg acaacaagcg cggctactac accagcttca tccaagtcac cgccacgctc
     5701 ggactcttcg tctccctcgg cgtgatcctc ggcgtcaaga gaatgatgag cgacgccgac
     5761 ttcaaagcgt ggggctggcg cctgccgttt ctcatctcga tcgttctcgt gggcatctca
     5821 ctctggatcc gcatgacgtt gaaggagtcg ccactcttcg cgtcgatcaa agcgcagggc
     5881 aagtcgtcga tcgcgccgct gcgtgagagc ttcggcaact ggaagaacct tcgcatcgtg
     5941 ctgctcgtgc tcttcggtgc aacggcaggg caggggatcg tctggtacac cggccagttc
     6001 tacgcgctct cgttcctcca gaccgtgctg aaggtcgacc tcgcaaaggc cagcatcatc
     6061 gtcgccgcgg cgctgctgct ggcgatgccg ttcttcgtcg tcttcggcgc gctgtccgac
     6121 aagatcgggc ggctgaagat catgatggcc ggcaatctcc tcgctgcgat cacttacctg
     6181 ccgatctacc acgccatgca cgaccatgtc ggcgatccaa agaatccgaa catggccatc
     6241 ctgatcggtc tcgtcttcat tcaggtgatc tacgtgacga tggtctacgg accgatcgca
     6301 gcgttcctga tcgagtcgtt cccggcgaag attcgctaca cctccttctc gctgccctac
     6361 cacctcggca acggctggtt cggcggcacg ctcccactca tcgccggcct tctcgtcgag
     6421 cgaacgcacg acatcttcg
//
LOCUS       DATLVW010000680         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_525374, whole genome shotgun sequence.
ACCESSION   DATLVW010000680 DATLVW010000000
VERSION     DATLVW010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525374"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VNN25_19800"
     CDS             <1..383
                     /locus_tag="VNN25_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93833.1"
                     /translation="EHQRLGRVLDDLRYLRLHLLDGADLGIEGLEIGQGELRLNGVSF
                     RQASMAQRLRVAALLAMKSKPRLRILRLDEGERLDSESWSTLLRLASERGFQVWATAV
                     RDESALKVEIIDAECAEHAESAVA"
     gene            539..1162
                     /locus_tag="VNN25_19805"
     CDS             539..1162
                     /locus_tag="VNN25_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93834.1"
                     /translation="MAGALRLAIGAGFPFSEDGGDFAALGEFRSGYWMSDSEEWVYSM
                     AIVEGNLSAAKAYEVWKGREPIIADEVDPGRSHGFIHGTGTRQTERLHVGCYFPWKGE
                     RVKVTSFAADGSAVACSYHPGDKDGYHHEHKIKKRYRITRETVIEDRADRRERKKLHA
                     DLLKMKDAGVDIGPALDGIGAKSQKEYEVVPVKRIRECVERLKKSAS"
     gene            1357..>2771
                     /locus_tag="VNN25_19810"
     CDS             1357..>2771
                     /locus_tag="VNN25_19810"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HXH93835.1"
                     /translation="MTTFVDDLEEIEADCFAGGGGVSLGLQWANGRSPAIAINHDRDA
                     IAMHAANHPDAHHFTEDVWKVNPRRATCGRAVGLLWLSPDCKHFSRAKGGKPAEKGIR
                     SLAWVAIRWAIECKPRIIMLENVREFADWGPLTDDNRPCPLRKGLTFRRFVGRLRGLG
                     YTVEWRVLNAADYGAPTHRRRLFLIARCDGEPIVWPEPTHGDPKRIGTGLFDQDLQPW
                     RTAAECIDWNIPCPSIFDRKRPLAEKTMRRIALGIKRYVLDNRQPFIVCCNHGGEEFR
                     GSSIGDPFPTLTASRDAHGLVIPVVVQFAHGEGKVANGTWGEGRSKPINAPLGTIHSG
                     GGNHGLVAAFLTKHFGGVVGVPVSTPLPTVTMRGTQTQLTVANLVHFNYVEKQWSDIR
                     DPMRTITTGNHAALVYSFLCKYFGTAIGQPLDRPMGTQTSRDRYALVTVWVDGELYVI
                     VDIGMRMLRPRELARANALPD"
BASE COUNT          568 a          791 c          925 g          487 t
ORIGIN      
        1 aggagcacca gcgtcttggc cgcgtgctcg acgatctgcg ctatctccgg cttcacctgc
       61 tcgatggagc tgaccttggc atcgagggcc tggagatcgg gcagggcgaa ctgcgcttga
      121 acggggtcag cttccgccaa gcgtcgatgg cgcagcggtt gcgcgtggcg gcgctcttgg
      181 cgatgaagag caagccgcgg ctgagaattc tgagacttga tgagggggaa aggttggaca
      241 gcgagagctg gtccacgctg ttgcggctgg cttccgagcg cggattccag gtctgggcaa
      301 cagcggtgcg tgacgagtcg gcgctgaagg tggagatcat cgacgcggag tgcgccgagc
      361 acgctgaatc cgcggtggcg tgaggtacgg actagctcta ttggcaggat ggatgtgcga
      421 ggggagttgg acgatggcga agaaaaagga cgagtcgccg gtgcttcgtt tgctcagaac
      481 cgtctgggga aacgccaact ccgcaacgga tcgttcctgg gaacgcctca atcacgccat
      541 ggccggcgcg ctgagactgg ctatcggcgc cgggtttccg ttttcggaag acggtggtga
      601 ctttgcggcc ctcggcgagt tccgttccgg ctactggatg agcgattcag aagaatgggt
      661 ttattccatg gccatcgtgg aaggaaacct ctcggccgcg aaagcctacg aggtatggaa
      721 aggccgggag ccgatcatcg ccgacgaggt tgaccctggt aggagtcacg gattcatcca
      781 cggcaccggg actcgccaga cggagcggtt gcacgtcggt tgctacttcc catggaaggg
      841 tgagcgcgta aaggtaacga gcttcgcggc ggacggctca gcggtggcat gcagttatca
      901 ccctggcgac aaggatggct accaccacga gcacaagatc aagaagcggt atcgcatcac
      961 tagagagacc gtcattgagg atcgggccga ccgccgggag cggaagaagc tccacgccga
     1021 tctgctcaag atgaaagatg ccggcgtaga cattggaccg gcgctcgacg ggatcggggc
     1081 caagagtcaa aaggaatacg aggtagtgcc ggtcaaacga atcagggagt gcgtggagag
     1141 gctgaagaag tcagcatcct gaaccaacct ggcgcgttgc caggtcgccc cggcccgaat
     1201 cgaggctgta gccactggac agcaggcccg gggcttttga accatgccgg cacgtccagg
     1261 caacgagtcg tgaaggcgag gaggagtaag cccttgattt cacaaccgac tggacgtgtc
     1321 ggcgttcaag ccgcgagaga cgaaaggact agacctgtga cgacctttgt cgatgacctg
     1381 gaagagatcg aagccgactg cttcgccggc ggaggcgggg tcagccttgg cctccaatgg
     1441 gcaaacggcc gctcgcctgc catcgccatc aaccatgatc gcgacgccat cgcgatgcac
     1501 gccgccaatc acccggatgc ccatcacttc accgaagacg tctggaaggt caatcctcgc
     1561 agggcgacat gcgggcgagc ggtcgggctg ctttggctct ccccggactg caagcacttc
     1621 agccgtgcca agggcggcaa gccggcggag aagggaattc gctccctggc ctgggtggcg
     1681 attcgctggg cgatcgaatg caaaccgcgc atcatcatgc tcgaaaatgt gcgtgaattc
     1741 gcagattggg ggccgctgac ggatgacaac aggccgtgcc cgctccgcaa gggtctcacg
     1801 ttccggaggt tcgttgggcg actgcgaggc ctcggctaca ccgtggaatg gcgcgtgctc
     1861 aacgcggcgg actacggtgc tccgactcac cgtcgcaggc tcttcctcat cgctcgctgt
     1921 gacggcgagc cgatcgtgtg gccggaaccg acgcacggag acccgaagcg gatcggcacc
     1981 ggcctgttcg accaggacct ccagccgtgg agaacagcgg ccgagtgcat cgactggaat
     2041 atcccgtgtc cgtccatctt cgatcggaag cgtccgcttg ccgagaagac gatgcggcgc
     2101 attgcgcttg ggatcaaacg gtacgtgctt gacaaccggc agccgttcat agtctgttgc
     2161 aaccacggtg gcgaggagtt ccgcggatcg tcgatcggcg atccgtttcc gacgctgacc
     2221 gcgtcgcgag acgcgcacgg tctagtaatt cctgtggtcg ttcaattcgc gcatggcgag
     2281 ggaaaagtcg cgaatggaac atggggagaa ggaaggtcga aaccaatcaa cgccccgtta
     2341 gggacaattc acagtggcgg agggaatcac ggcctagtcg cggctttctt gaccaaacac
     2401 ttcggcggcg tcgtgggcgt gccagtcagt acgccattgc cgacggttac gatgcgcggc
     2461 acgcagacgc agctcaccgt ggcgaacctg gttcacttca actacgtcga gaagcaatgg
     2521 tctgatatcc gcgatccgat gcggacgatc acgaccggaa accacgcggc cctcgtctac
     2581 agcttccttt gcaagtattt cggcaccgca atcgggcagc ctctcgatcg ccccatgggt
     2641 acgcagacct cgcgggaccg ttacgccctg gtcacggtct gggttgacgg cgagttgtac
     2701 gtcatcgtcg acatcggaat gcggatgctg agacctcgcg aactggcgcg agcgaacgcg
     2761 ctgcccgaca g
//
LOCUS       DATLVW010000681         5386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_111874, whole genome shotgun sequence.
ACCESSION   DATLVW010000681 DATLVW010000000
VERSION     DATLVW010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111874"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VNN25_19815"
     CDS             complement(<1..888)
                     /locus_tag="VNN25_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXH93836.1"
                     /translation="MESDVATLAMLIGALVPYDGTIELRVPGVRAARASHTNQEPMYY
                     LQRSTLCIVAQGAKIVMIGGDTYGYKAGQMAVYSIDVPMAGRVTRASYSEPYLLLMID
                     LDAEKIAELAPKVFPHGLPQPRDSRSLYVGDAGPHIIDAATRLLELMAQPIDAELLAP
                     LVRDEILIRLLRSPLGSRVAHIGRAGSSVQRIANAVSRLRANFDQIVNIEELAKLVNM
                     SVTSFHRQFKAVTGMSPLQYQKTLRLQEARRLMLATMIDAGSAGRRVGYGSASQFSRE
                     YNRFFGSAPAKDINRLREVG"
     gene            complement(937..1914)
                     /locus_tag="VNN25_19820"
     CDS             complement(937..1914)
                     /locus_tag="VNN25_19820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH93837.1"
                     /translation="MKHISIGGLDVSRIGLGAMSMAGYYNPGAVSDAESIRTIHRALD
                     LGVTHIDTAEIYGPYANEELVGRAIKGRRNEVVLATKFGIVSHAGGGPGIIDSSPANV
                     RAAIEGSLKRLGTDHIDLYYQHRVDPNTPIEDTAGALASLVAEGKVRFIGLSEAGADT
                     IRRAHAVHPVAALQTEYSLWTRDPEAELLPLLRLLGIGFVPYSPLGHGFLTGTIRSND
                     ELSDDDWRKTNPRFTGENLQHNLRIVHEVKAVAVEAGATPAQVALAWLLAQGDDIAPI
                     PGTKRVARVEENTAADQVALSPAQIERLNNLAPAAGERHDQGNMAVIEH"
     gene            complement(2237..4846)
                     /locus_tag="VNN25_19825"
     CDS             complement(2237..4846)
                     /locus_tag="VNN25_19825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH93838.1"
                     /translation="MSDVSAGVRLGHYELLARIGEGGMGEVWRARDVRLDRSVAIKLL
                     SSNLAASPQLRQRFEREARAISALNHPHICTLHDVGEEGGRSYLVMELLEGETLADRI
                     VRGPLPLSDVVRYGSQIADALDRAHRAGIIHRDLKPGNIMITRSGAKLLDFGLAKAAS
                     PVMSVDGPTIQKPLTQEGTILGTVQYMAPEQLEGQDADARTDIFALGAVLYEMTTGRR
                     AFEGKTRTSLIAAIVGSDPPPIRNSQPLTPTALEHVIATCLEKEPDRRWQSAHDVASE
                     LSWIAGGSAIDQAPVRRARRRLATIVIIALALIAAAAIGMLVRERTKAPEPLAFAVMP
                     PHGFSLWSAAISPDGRSIALVAGTPTESSLWVRRLGEINGTRLTAAGAFRFVIRGPFW
                     STDSQWIGFVSDGRVMKIRATGGAVETLAKLPGYYCGAAWNAKGDILYAPRFGTGLYR
                     VPSGGGEAVPITTLDPKRRETLNGWPRFLPDGDHFLYVSHTIGEEPNEIYAGSLTSKM
                     KQKVLQADALVGVWDGKLLFVRNGAIYAQPFDTKTLAVSGEAHSVIDEVLFFETAANA
                     STSMADDGAILYPPASNALMKTEIGWYDDSGRPLQKLFELPAFASDLRLTRDESRIAM
                     ARWDPRKGANDIVIRDVARGVETRVTGGLSDNGNPVWSRDGSRIYFASDRDGAYNIYS
                     QTDDGVSPPQHVWKGGDDNVSHDVSPDGTILLAGLYLEKTKNDIWIVPLNGGAPRPWI
                     ATDANELYPRFSPDGKWVVYSSDRSGRLEVYVAAFPEGRSFQVSTAGGRGPDWDADGK
                     HVVFEQDDDSLCSVAISTSGTTVVPSKPVVLFQLPKTINGWWRSRTAKRFLGRTVLDP
                     AETVQVMHYIKGW"
     gene            5189..>5386
                     /locus_tag="VNN25_19830"
     CDS             5189..>5386
                     /locus_tag="VNN25_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93839.1"
                     /translation="MPFEHDFLISYAHIDNEALIAGDHGWISELHRLLEIRVGQVRGE
                     KPKIWRDPKLQGNDIFADTILE"
BASE COUNT          926 a         1696 c         1735 g         1029 t
ORIGIN      
        1 ccccacttcg cgtagccggt tgatgtcttt cgcgggcgcg ctcccgaaga agcggttgta
       61 ttcgcggctg aactgggacg cgctgccgta gccgacgcgc cggccggcac tgcccgcatc
      121 gatcatcgtg gccagcatca gccgtcgcgc ttcctgcagc cggagcgttt tctggtattg
      181 cagtgggctc atgcccgtga cggctttgaa ctgccggtga aatgacgtga cgctcatgtt
      241 caccagcttg gccagttctt cgatgttcac gatctggtcg aaattggctc gcagccgcga
      301 caccgcattc gcgattcgct gcacgctcga accggcccgg ccgatatggg caacacgact
      361 cccgagcggg ctgcggagca ggcgaatcag gatctcgtcg cgtacgagtg gtgccaggag
      421 ctcggcatcg atgggctgcg ccatcagctc aaggagccgc gtcgctgcat cgatgatatg
      481 cgggccggca tccccgacat agagcgagcg gctgtcccgc ggttgcggca ggccgtgcgg
      541 aaacactttg ggagcgagct ccgcgatctt ctccgcatcg agatcgatca tgagcaacag
      601 gtacggttcg gagtaactgg cccgcgtgac ccggccagcc atcgggacgt cgatcgagta
      661 caccgccatc tgtccggctt tgtaaccgta ggtgtcgccg ccgatcatca cgatcttcgc
      721 gccctgcgca acgatgcaca acgtcgagcg ctgtaggtaa tacatcggct cctgattcgt
      781 gtgtgacgct ctggcggcgc gcacaccagg gacgcgcagc tcgatggttc cgtcgtatgg
      841 cactagcgcg ccgatcaaca tcgccagcgt cgcaacgtcg gactccattc gtctcgcacg
      901 catcatccag ccctcgctcc attctcttac agttgatcag tgctcgatga cggccatgtt
      961 cccttggtcg tggcgctcgc cggcggccgg cgcaaggttg ttcagccgtt cgatttgcgc
     1021 gggactcagc gcgacctgat cggcggcagt gttctcctcg acgcgggcga cccgcttggt
     1081 tcccggaatc ggggcgatgt cgtctccctg agcgaggagc cacgcaaggg ccacttgtgc
     1141 aggcgtcgcg cccgcctcaa cggccacggc cttcacctca tggacgatgc gcaggttgtg
     1201 ctggaggttc tctccggtaa agcgcgggtt ggtcttccgc cagtcatcgt cggacagctc
     1261 gtcgttggag cggatcgtgc cggtgaggaa accgtggccg agcggcgagt agggcacgaa
     1321 gccgatgccc agcaggcgca ggagcggcag cagttcggct tccgggtcac gggtccagag
     1381 ggagtattcg gtctgcagcg cggcgaccgg gtgcacggcg tgagctctgc ggatcgtgtc
     1441 ggcaccagcc tccgataacc caatgaaccg caccttcccc tcggcgacca gcgacgccag
     1501 cgctccagcc gtgtcttcaa tgggcgtgtt cgggtccacc cggtgctggt agtacagatc
     1561 gatgtggtcg gtgccgaggc gcttcagtga gccctcaatc gcggcgcgga cgtttgccgg
     1621 actgctgtcg atgatgcccg ggccaccgcc ggcatgcgag acgatgccga acttcgtcgc
     1681 taacacgacc tcattgcgcc ggcccttgat ggcgcggccg acgagctcct cgttcgcgta
     1741 aggaccgtag atctcggcag tgtcgatgtg ggtgacgccg aggtcgagcg cccggtgaat
     1801 cgtgcggatc gactccgcgt cgctgactgc accggggttg taatagccgg ccatcgacat
     1861 ggcgccgagt cctatgcggg atacgtcgag tccaccgatc gagatgtgct tcattgtgtc
     1921 gatcccttct cgtaaaaccg cgacgtctac tcacaaggtc gcggcgttgt ctgcgtttct
     1981 cttcaccttc gccagcccta gagataacgc ctcgggacct tcgaatcgtt gatcgatcgt
     2041 tccaatgttt tgcacgatcc ttccgaggta tgggctccgc tccgttcctg gcggcgtgac
     2101 atacagtcag acgcgcgtgc cggccgcccg aggttcctgc cgccggtccg caaactctcg
     2161 acgcgcagag ttagacgtgc cactccatac gatcagcgcg tcctggcgag gagcgccggt
     2221 gtgttggaag cggctgttac cagcccttga tgtagtgcat gacctgtaca gtctcggccg
     2281 ggtcgaggac agtgcggccg aggaagcgct tggcggtgcg gctgcgccac catccgttga
     2341 tcgttttggg gagctggaaa agcacgaccg gcttggaagg aacgacagtc gttcccgatg
     2401 tgctgatggc caccgagcag aggctgtcgt cgtcctgctc gaagaccacg tgcttgccgt
     2461 cagcatccca atccggtcct ctcccgcccg cggtggagac ctggaacgag cgtccctcgg
     2521 gaaaggctgc gacgtaaacc tccaggcgtc cggagcggtc ggacgagtag acgacccatt
     2581 tcccatccgg tgagaaccgg gggtagagct catttgcgtc ggtggcaatc cacggtcgcg
     2641 gcgcgccgcc gttcagcgga acgatccaga tatcgttctt tgttttttcc aaataaagcc
     2701 cggccaggag gatcgtcccg tccggcgaga cgtcgtgcga aacgttgtcg tcgccgcctt
     2761 tccaaacgtg ctgtggcggg ctgacgccgt cgtcggtctg ggagtagatg ttgtacgcgc
     2821 cgtcacggtc cgatgcgaag tagatgcgcg agccgtcgcg ggaccagacc gggttcccgt
     2881 tatcggagag acctcccgtc acgcgggtct ccacgcctcg agcgacgtcg cggatgacaa
     2941 tgtcgttagc tccctttcgc gggtcccacc tggccatggc gatcctggac tcgtcgcgag
     3001 tcaggcgcag atcggaagca aatgccggta gctcgaaaag tttctgcagc ggccgtccgg
     3061 agtcgtcgta ccagccgatc tccgttttca tgagggcgtt ggacgcggga gggtagagga
     3121 tggccccgtc gtccgccatc gaagtgctgg cgttggcagc cgtctcgaag aacagaactt
     3181 cgtcgatgac cgagtgcgcc tcaccgctca cggcgagcgt ctttgtgtcg aacggctggg
     3241 cgtagatcgc tccgttccgg acgaagagca gtttcccatc ccaaacaccg accagcgcat
     3301 ccgcctgcag cactttttgc ttcatcttcg aggtcagcga gccggcgtag atctcgttcg
     3361 gctcttcgcc gatcgtgtga gatacataca ggaaatgatc gccgtcgggt agaaaccgcg
     3421 gccagccgtt cagcgtctcg cgccgcttgg gatcgagcgt ggtgatggga acggcctcac
     3481 cgccgccgga cggcacccga tacagccccg tcccgaatcg cggcgcgtag aggatgtcgc
     3541 ctttcgcatt ccatgccgcg ccacaatagt agccgggcag cttggccaac gtctccaccg
     3601 cccctccggt ggctcgtatc ttcatcaccc gaccgtcgga gacgaaaccg atccattgag
     3661 agtcggttga ccagaagggc ccccggatga cgaatcggaa cgcgccggcc gcagtgagcc
     3721 ttgtgccgtt gatctcgccg aggcggcgta cccacagcga cgattcggta ggcgttccag
     3781 cgacgagggc gatggatcgt ccatcggggg agatggcggc actccacagc gagaagccgt
     3841 gcggcggcat gaccgcgaag gccagcggct cgggcgcctt cgtccgctcg cgcaccagca
     3901 ttccgatcgc agcggcggcg atgagtgcga gcgcgatgat gacgatggtg gcaagccggc
     3961 gcctggcgcg acgtaccggc gcctgatcga tggcgctccc gccagcgatc cacgacagct
     4021 cgctggctac gtcgtgcgcg ctctgccacc gccggtcggg ctccttttcc aggcaggtgg
     4081 cgatgacatg ctccagcgcg gtcggcgtaa gcggttgcga gttgcggatg ggcggcggat
     4141 cgctgccgac gatcgctgcg atgagtgacg tgcgcgtctt gccttcgaag gcacggcggc
     4201 cggtggtcat ctcgtacagc accgcgccga gggcgaagat gtcggtgcgg gcgtcggcgt
     4261 cctgcccctc gagttgctcg ggagccatgt actggaccgt gccgaggatc gtcccttcct
     4321 gcgtgagcgg cttctggatg gtcgggccgt cgacagacat cacaggactc gcagccttcg
     4381 ccagcccgaa gtcgagcagc tttgcgccgc tcctggtgat catgatgttg ccgggtttga
     4441 ggtcgcggtg gatgatcccc gcgcggtgtg cgcggtcgag cgcgtcggcg atctgcgagc
     4501 cgtatcgaac gacgtccgaa agcgggagcg gtccccggac aatacggtcg gcgagcgtct
     4561 ccccctccag caactccatc accaggtagc ttctgccgcc ctcctcgccg acgtcgtgca
     4621 gtgtgcagat atgaggatgg ttcagcgcgg agatggcccg cgcctcccgc tcgaaccgct
     4681 gccgcaactg cgggctcgca gccaggttcg acgacagcag cttgatggcg acgctgcggt
     4741 cgagccgcac gtcgcgcgcc cgccacacct cccccatccc gccctcgcca atgcgcgcga
     4801 gaagttcgta gtgaccgagg cgaacgccgg ccgaaacgtc ggacatgatg atgagttcaa
     4861 ggtgattatg agcagtccgg cggatgaggt gaagtctttt ctatatggga agaacccatg
     4921 cgcaacaagt ccggcgcact ggcgccttcg ctacgccgcc gctgcgcatg actgatcgcg
     4981 ttcatttgtc cggaaaccaa cgatgtactg cggagtgacg gcttacttct acgtcgatcg
     5041 cggacgccgc tacttcgacg accgaagaac accgcagttg gtccacaaag aaacagatcg
     5101 cctttctcac gaaggagagg ggtagggaaa tgcctcgctg cgtcactcct ctagtggtcc
     5161 ctcaatccat taatcgttcc ctactcgcat gccattcgag cacgactttc tcatcagcta
     5221 tgcgcacatc gacaacgaag cattgattgc cggggatcac ggctggatct cggaactgca
     5281 caggctgctg gagattcggg ttgggcaggt gcggggggag aagccgaaga tctggcggga
     5341 tccgaagctg cagggcaacg acatctttgc tgacacgatc ctcgag
//
LOCUS       DATLVW010000682         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_607369, whole genome shotgun sequence.
ACCESSION   DATLVW010000682 DATLVW010000000
VERSION     DATLVW010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607369"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VNN25_19835"
     CDS             <1..1224
                     /locus_tag="VNN25_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93840.1"
                     /translation="PPLPPLPPPPDIPIATAPQPTPPPAAVPPAAQPVPPLSLSPVAA
                     SGYDRRDALPNVYLADGQASIRLRKLIKNVLFESQIDYKFVTGDISTFLRYKYYAENF
                     TYKIGVFDSISFGAVGSHTDEFERVRGGLLLLTFPRDYNHRYFTLLQDDRLTFGDVTR
                     VDNRKNNIYTKFGYQYGTEFDEQLNAIVGETRGRQVPVLTVFREVGPQKFSYAAALTE
                     SAKISTGDYRYTKVETEGIRRWDITGTQFIVTRAHFGTFLTKDTLCAKDDVTTPQVEC
                     FSIPGYEMFGLGGRDALRSLSTSNSVSAGTNEFHVTNEYFVPVFRNRDFRYRAVHFNT
                     MYAIGYTGFGNVAFTTRDVFKTRDFAVDAGLGTEMALTVRDFDVLLSVLYAHTFRAPE
                     ERKGNKVQFSIHTIR"
     gene            complement(1325..1813)
                     /locus_tag="VNN25_19840"
     CDS             complement(1325..1813)
                     /locus_tag="VNN25_19840"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXH93841.1"
                     /translation="MSANKHPHPMTWAGLLLAVFFAVSAIAEPPVTHDVTAQFVSAGV
                     LLEGFHAVEVGGIVILRGHAIDTTSAEQAAVVAQNLGYSRVANLIQMDAVPDDARIAR
                     AAERGLALQRGLDGTQIVVASTNGVVHLTGTVSNELQKDMAVHVVRNIDGVRAVQMSL
                     KR"
     gene            complement(1880..2116)
                     /locus_tag="VNN25_19845"
     CDS             complement(1880..2116)
                     /locus_tag="VNN25_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93842.1"
                     /translation="MSDLLRLPIPDTRQPSITMHGTGFAFMMNVLRRITALLAMVAII
                     GLLALLVWQVEMHHRHGSASDEPPTLVALLGRAA"
     gene            2225..>3212
                     /locus_tag="VNN25_19850"
     CDS             2225..>3212
                     /locus_tag="VNN25_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXH93843.1"
                     /translation="MKAILIREHGGLDKLTMADVPDPVPRPGEAVVRVRAVALNHLDL
                     WLRRGVPGHTFPLPMIPGSEVAGVIEQVDDPNWRAGDEVIVAPGYSCGHCVACLSGND
                     PLCRHYGLFGETISGGAAEKIAVPVRNLIRKPTALSFVQAAAFPLDMLTAWHMLVARA
                     QLRMGESVLVHAGGSGVGSAAIQIAKLFGATVYATAGIVGKASRAEALGADATIVYTE
                     TNFLDEVRRLTGKRGVDVVFEHVGGETFERSLKALVRGGRLVTCGATTGGKVNLNLQA
                     VFFKQLEIIGSTMGGSGDMLDAWKEVGRIRPVVDRVMKLSEIASAHSLLEGRG"
BASE COUNT          615 a         1073 c          940 g          584 t
ORIGIN      
        1 cccccgcttc ccccgttgcc accgccaccc gatattccga tcgccacggc ccctcagccc
       61 acgcctccgc cggccgccgt tcctccggcc gcgcagccgg tgccgccgct ctcgctctcg
      121 ccggtggccg ccagcggcta cgaccggcgc gacgcgctgc ccaacgttta tctcgctgac
      181 gggcaggcca gcatccggct gcgaaagctg atcaagaacg tgctgttcga gagtcagatc
      241 gactacaagt tcgtcaccgg cgacatcagt acgtttcttc gctacaagta ctacgcagag
      301 aacttcacct acaagatcgg cgtgttcgac tcgatctcgt tcggcgccgt cggcagccac
      361 accgacgaat tcgagcgcgt gcgcggtggt ctgctgctgc ttacgtttcc gcgcgactac
      421 aaccaccgtt acttcacgct cctgcaggat gaccggctca cgttcggcga cgtcacgcgc
      481 gtcgataacc gcaagaacaa catctatacg aagttcggct accagtacgg caccgagttc
      541 gacgaacagt tgaacgcgat cgtcggcgaa acacgcggcc ggcaggtgcc cgtgctcacc
      601 gtgttccgcg aggtcggccc tcagaagttc agttacgccg ccgccctaac cgagtcggca
      661 aagatctcca ccggcgacta caggtacacg aaggtggaga cggaaggcat ccgccgctgg
      721 gatatcaccg gcacacaatt catcgttacg cgcgcacatt tcggcacgtt cctgacgaag
      781 gacactctct gcgcaaagga cgacgtcacg actccccagg tcgagtgctt ctcgatccca
      841 ggctacgaga tgttcggact cggaggacgc gacgcgctcc gttccctctc cacctcgaac
      901 agcgtttcgg ccggcacgaa cgagttccac gtcacgaacg agtatttcgt tcccgttttc
      961 cgcaatcgcg atttccgcta ccgcgcggtg cacttcaaca cgatgtatgc aatcggctac
     1021 accggcttcg gcaacgtcgc attcacgacc cgcgatgtct tcaaaacccg cgatttcgcc
     1081 gtcgacgccg gcctcggcac cgagatggcc ctcaccgtcc gtgacttcga tgtcctcctc
     1141 agcgtcctct acgcccacac attccgcgcc ccggaagagc gcaaaggaaa caaggtccag
     1201 ttctcgattc acacgatccg ctgaggggga gtggagagca gagagcagag ataagagaca
     1261 agagatcaga gggctcgaac cacagagact aatctctgat ctctgttccc ctgatctctg
     1321 cccttcaccg cttcaacgac atctgcaccg cgcgcacgcc gtcgatgttc cggactacgt
     1381 gaacggccat gtctttctgc agctcgttgg aaaccgttcc ggtcagatgg acaacgccgt
     1441 tggtcgaggc gaccacgatc tgcgttccat cgagtccgcg ctggagcgcc aatccgcgct
     1501 cggcggcgcg ggcgatccgc gcatcgtccg gaacggcatc catctggatg aggttggcca
     1561 cccggctgta gccgaggttc tgcgcgacga cggcggcctg ctcggcagac gtcgtgtcga
     1621 tcgcgtgccc gcgaaggatg acaattccgc caacctcgac cgcatggaat ccttccagga
     1681 gcacacccgc gctaacgaac tgggcggtga catcgtgcgt gactggcggc tccgcaatgg
     1741 cggagaccgc gaaaaacacg gccagtagga gcccggccca cgtcatcgga tgcggatgtt
     1801 tatttgcgct catgagagca aataaagcat cgttgatgcc aatgttaaac gccttttgct
     1861 ggagagactt agcccgagct caagctgctc tgccaagcaa ggccacaagc gttggcggct
     1921 catcgctggc ggagccatga cgatggtgca tctccacctg ccagaccaac agagcaagga
     1981 ggccgataat cgcaaccata gccagcagcg cggtgatgcg tcggagcacg ttcatcatga
     2041 aggcaaagcc tgtgccgtgc attgtaatgc tgggttggcg ggtgtcggga atcgggagtc
     2101 ggagcaggtc gctcaagcaa aagcaaccac agagacacag agaacacaga gaggttctct
     2161 gtgacctctg tgcctctgtg gtgaccggtt ttcaaattga caacccagct atcatccccg
     2221 ccgcatgaaa gcgattctga ttcgcgagca cggtggcctc gacaagctga ccatggccga
     2281 tgttcctgat cctgtcccgc gaccgggcga ggcggtcgtg cgtgtccgcg ccgtcgctct
     2341 caatcacctc gatctctggc tccgccgagg agtgcccggg catacgtttc cactgccgat
     2401 gattccgggg tcggaagtgg cgggcgtcat cgaacaggtc gatgatccga actggcgcgc
     2461 cggcgacgag gtgatcgtcg cccctggtta ctcctgcggt cattgcgtcg cctgcctctc
     2521 cggcaacgat ccgctctgcc gccactacgg tctcttcgga gagacgatca gcggcggcgc
     2581 ggcggagaag atcgccgtcc ccgtccgcaa tctgatccgc aaaccaaccg cgctttcgtt
     2641 tgttcaggcc gccgcgtttc cactcgacat gctcaccgcc tggcacatgc tcgttgcccg
     2701 cgcccagctc cgcatgggcg agtccgtcct cgttcacgcc ggcggttccg gcgtcgggag
     2761 cgctgccatt cagatcgcca agctgttcgg cgcgaccgtc tacgccaccg ccggaatcgt
     2821 cgggaaagcc tcccgcgccg aagccctcgg cgccgacgcc accatcgtct acaccgagac
     2881 gaacttcctc gatgaagtgc gccggcttac tggcaaacgc ggcgtcgacg tcgtcttcga
     2941 gcacgtcggc ggcgagacgt tcgagcgctc gcttaaagcc ctggtgcgcg gcggccgcct
     3001 cgtcacctgc ggcgccacga cgggcggcaa ggtgaacctg aacttgcagg cggtcttctt
     3061 caagcagctg gagatcatcg gcagcaccat gggcggcagc ggcgacatgc tcgacgcctg
     3121 gaaggaggtc ggccgcatcc gcccggtggt ggaccgggtg atgaagctct ccgagatcgc
     3181 ttcggcccac tcgctcctgg aagggcgagg gg
//
LOCUS       DATLVW010000683         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_482269, whole genome shotgun sequence.
ACCESSION   DATLVW010000683 DATLVW010000000
VERSION     DATLVW010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482269"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VNN25_19855"
     CDS             <1..129
                     /locus_tag="VNN25_19855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HXH93844.1"
                     /translation="QIFTIFIITVAAGEAAVGLAIIIQLYRLKSTINVDEARELNG"
     gene            122..2293
                     /gene="nuoL"
                     /locus_tag="VNN25_19860"
     CDS             122..2293
                     /gene="nuoL"
                     /locus_tag="VNN25_19860"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXH93845.1"
                     /translation="MVNATVLLLIPLLPALGALINGLRAAASPHTPKNRAFTNVIALG
                     STGLSAILAAWTVIVYVGGNTSAAFQHAYYTWIPAGLGHAAGNTIANFAVDFAFRIDT
                     LSCTMLLIVTWVGFLIHVYATGYMAHEEGYTRFFTYLNLFMFMMLLLVLGANYMVMFV
                     GWEGVGLCSYLLIGFYYDKNFAADAGKKAFVANRIGDFGFILGVFLIFNTFGTADFGK
                     VFALASAHPQAYAGIATTICLLLFVGACGKSAQIPLYVWLPDAMAGPTPVSALIHAAT
                     MVTAGVYMVVRSNVLFRLSPDAMLVVATVGAVTAIFAATIGIAQNDIKKVLAYSTVSQ
                     LGFMFLAAGVGAFTAAIFHVMTHAFFKACLFLGAGSVIHGCGGEQDMRKMGGLRKYMP
                     KTRMTMLIACIAIAGFPPLAGFFSKDEILATTYEHHGFLYFLGMVAATCTAFYMFRLY
                     FMTFSGNYRAAGVVGQPEPEPEHAGHDVIPGSLHLDDGDVHSDKAHGHHAEAHTPHES
                     PWSMTGVLWILAILAVIGGIVGVPQMLGGTHPTIFQRWLLPVLQPLGGEHFEFAEASR
                     MQEAILIASSVVVAFLGWLLAYVLYKKDAAFARATRMATRFAFLHRLLENKYYVDEFY
                     NFVFVGGTLAFSRALSWFDANIVDGIVNGVRNLTVVALGHGSWLFDRYIVDGAVNGVG
                     FSARGGSRFFRRMQSGFVQNYALVMGGGIVLIAAVYLFTKP"
     gene            2499..>2862
                     /locus_tag="VNN25_19865"
     CDS             2499..>2862
                     /locus_tag="VNN25_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HXH93846.1"
                     /translation="MGILNIVTYIPLIGAVLILLFLPKENGKAIRYTATFVAVADFIA
                     SLYLWFNFDPKGTGDGMWQFRFIHSWIPSLNVKYDFGIDGISLLLILLTTFMGIIAVV
                     SSFAAIDHRQKEYYILLLL"
BASE COUNT          495 a          929 c          804 g          634 t
ORIGIN      
        1 cagatcttca cgatcttcat cattaccgtt gcagcagggg aggccgcggt cggcctggcc
       61 atcatcattc agttgtaccg cctcaagagc acgatcaacg tcgatgaagc gagggagttg
      121 aatggttaac gcaaccgtcc ttctgctcat cccgctcctc cccgcgcttg gcgcgttgat
      181 caacggcctg cgcgctgcgg cgtcgccaca tacgccgaag aatcgcgcgt tcacgaacgt
      241 catcgcactc ggatcgacgg gtctttccgc gatcctcgcc gcttggaccg tgatcgtcta
      301 cgtcggcggc aatacctcgg cggcgtttca gcacgcgtac tacacatgga tcccggcagg
      361 actcggccac gctgcgggga atacgatcgc caacttcgcc gtcgacttcg ccttccgcat
      421 cgatacgctc tcctgcacga tgctcctcat cgtcacgtgg gtcggctttc tgatccacgt
      481 ctacgccacc ggctacatgg cgcacgaaga ggggtacacg cgctttttca cctacctcaa
      541 tctcttcatg ttcatgatgt tgctcctggt cctcggggcg aactacatgg tcatgttcgt
      601 cgggtgggaa ggggtcggtc tctgctcgta tctgctgatc ggcttctact acgacaagaa
      661 cttcgcggcg gacgcgggca agaaagcctt cgtcgccaac cgcatcggcg acttcggctt
      721 catcctcggc gtcttcctca tcttcaacac cttcggcacc gcggacttcg gaaaagtctt
      781 cgcactcgcc agcgctcacc cgcaggcgta cgcgggaatc gccaccacga tctgcctgct
      841 cctcttcgtc ggcgcgtgcg gtaagtcggc gcagatcccg ttgtacgtgt ggctccccga
      901 cgccatggct ggcccgacgc cggtttccgc gctgatccac gccgcgacga tggtcactgc
      961 tggcgtctac atggtggtcc gttcgaacgt cctcttccgg ctgtcacccg atgcgatgct
     1021 cgtcgtagca actgtcggcg cggtcacggc gatcttcgcg gcgaccatcg gcatcgcgca
     1081 gaacgacatc aagaaagtcc tcgcctattc gaccgtctcg cagctgggct tcatgttcct
     1141 cgctgccggc gtcggcgctt tcaccgcggc catcttccac gtgatgacgc acgcgttctt
     1201 caaagcctgt ctcttcctcg gcgccggatc agtcattcac ggctgcggcg gtgagcagga
     1261 catgcgcaag atgggcgggc tgcgcaagta catgccgaag acgcgcatga cgatgctgat
     1321 cgcgtgcatc gccatcgcag ggttccctcc gctggccggc ttcttctcga aggacgagat
     1381 cctggccacg acctacgagc atcacggctt cctctacttc ctcggcatgg tcgccgcgac
     1441 ctgcacagcc ttctacatgt tccgcctcta cttcatgacg ttctccggca actaccgcgc
     1501 tgctggtgtc gtgggccagc ccgagcccga accggagcat gccggccacg acgtcattcc
     1561 tggctctctc catctcgatg atggcgacgt gcactctgac aaggcgcacg gtcatcacgc
     1621 cgaggcgcac acaccgcatg agtcgccgtg gagcatgacc ggagtccttt ggatcctggc
     1681 gattctcgcg gtgatcggcg gcatcgtcgg cgttccgcag atgctcggag ggacacaccc
     1741 gacgatcttc cagcgatggc tcctgcccgt tctgcagccg cttggtggcg agcacttcga
     1801 gttcgccgag gccagccgga tgcaggaagc gatactgatt gcctcgtcgg tcgtcgtggc
     1861 cttcctcgga tggctgctgg cctacgtcct ttacaagaaa gacgctgcgt tcgctcgggc
     1921 gacgcgaatg gcgacgcggt tcgcgttcct tcaccgcctg ctcgagaaca agtactacgt
     1981 cgacgagttc tacaatttcg tcttcgtcgg cggcacgctg gcgttcagcc gggcgctctc
     2041 gtggttcgat gcgaacatcg tcgacggcat cgtcaacggt gtgcgcaatc tcacggtcgt
     2101 cgcgctcggc cacggctcgt ggctcttcga ccggtacatc gtcgatggcg ccgtcaacgg
     2161 agtcggcttc agcgcgcgcg gcggctcgcg tttcttccgc aggatgcaga gcggattcgt
     2221 ccagaactat gcgctggtga tgggcggtgg catcgtcctc atcgccgcgg tctatttgtt
     2281 tacgaaacca tgaagaggca gagatcagag acgagagacc agagatgagg gcgcaaccag
     2341 accgtctcct gatctctagt ctctgattct tcggagaaaa ggtctttttg tttacgaaac
     2401 tatgaggggg agaagagatc agagatgaga gatcagagat caggatgccg tccgggttcc
     2461 tgatctctga tctctagtca ctgatctctc cacagaagat gggcattctc aacatcgtca
     2521 cgtatatccc gctgatcggc gcggtcctca ttctcttgtt tctcccgaaa gagaatggca
     2581 aggctatccg ctacacggcc acgttcgttg ccgtcgccga cttcatcgcc tcgctctacc
     2641 tctggttcaa cttcgatccg aaagggaccg gcgacgggat gtggcagttc cgcttcatcc
     2701 actcctggat tccttcgctg aacgtcaaat acgacttcgg catcgacggc atctcactcc
     2761 tgctcatcct tctgacgacg ttcatgggca tcatcgccgt cgtctcctcg ttcgccgcca
     2821 tcgatcaccg gcagaaggag tactacatcc tgctgctcct gc
//
LOCUS       DATLVW010000684         4828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_607478, whole genome shotgun sequence.
ACCESSION   DATLVW010000684 DATLVW010000000
VERSION     DATLVW010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607478"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VNN25_19870"
     CDS             complement(<1..721)
                     /locus_tag="VNN25_19870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH93847.1"
                     /translation="MGNTPEVTLNRWWRVLGGLFMNLALGSLYAWSVFVAPLEKEFGW
                     KRADTSLVFTWAIVVFALSFVLAGRLQDKLGPFMVSVVGSILLTVGFLLSSQTHSLTY
                     LILCFGVLVGIGNGFGYATPIPVMAKWFPDKRGLAVGLAVAGYGGGSAIFGPLANLWL
                     IPTFGWRNTFLVLGAVFFVMTMIGAFLLKNPPVGYRPEGWTPAPAAKAAGTTYEFTPG
                     QMWRTPSAWFMWIAYALGCAAG"
     gene            complement(779..1891)
                     /locus_tag="VNN25_19875"
     CDS             complement(779..1891)
                     /locus_tag="VNN25_19875"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXH93848.1"
                     /translation="MSEKHGNSRICGPSILTFLAGAFLLLMPVVVQAQAVIKVNDNVN
                     IRFGGLLQSWGDSLQDATSRGYGNNLFIRRMRFLVAGQVTPNLTFFFESDNPNLGKSP
                     KALGTGFMTQDAYVEWKPTGSNAFALDAGLMLVPLCRNCLNSAGTLLALDYGSNSFIE
                     SGATQSSVGRDTGFQAKGYLDGGRLEYRAALLQGMRLAGARNPLRTTARLQYNFYDTE
                     VGGFFYPGFYMGNKKIVAIGAGTDHQSDYQAYSGDLFLSLPNAAKTNALNADVQLLSF
                     DGNKTFPAMAQQHDLMLQAGYYVGKLKIQPFLRFEEQNFVRSADNGKDTKRVQAGVAW
                     LPNGHNFNVKGAYTRVDPRLGNKTNELTVQLQFFYF"
     gene            2393..4444
                     /locus_tag="VNN25_19880"
     CDS             2393..4444
                     /locus_tag="VNN25_19880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXH93849.1"
                     /translation="MHSSKLIRATVLLLAAFIALAATPPPSHGLDLAGMDKTVKPGDD
                     FYGYANGTWAKVTPIPPDRSSYGVFTILDEKANQRTSDLIRETEKAKAPAGSEVRMIG
                     DYYAAFMDEKSIEARGLKSVQPELDEIAAIADKQALANVMGSQLRADVDALNNTNFYT
                     DRPFGLWVSPDFDNPNRNIGYLLQGGLGMPDRDNYLSKEKNDVALQEKYRQHIAAVLT
                     LAHIADAAGKAGRIYDLERKIAEAHGNRTDSEDVHKANNRWPLQQFPVKAPGLDWAAY
                     FKGAGLSGQTEIMVWQPSAAVGIAALAGSEPLSLWKDYLTFHAIDRASGLLPKAFADE
                     RFKFYGTALTGAPQQRARWKRAVSATSGALGDAVGKLYVKHYFPPSAKKAAQTMVSNI
                     VAAFSRRIDNLDWMKPETRTKAKAKLSTLYIGIGYPEHWRDYSGLVIKRDDALGNEQR
                     SELFDYRAAVARLGKPVDKSSWAMEPQTVNAVNLPLQNALNFPAAILNPPFFDEKADA
                     VLNYGAIGTVIGHEISHSFDDQGSQFDAEGRLLNWWTKEDFAHFSAAADRLAAEFDTY
                     EPLPGLHVNGKLTLSENIADVAGLSASYDGYRRFYGDKAGPSAAGFTGDQRFFLAFAQ
                     AWRSKLRPEALRNSLKTNGHAPGQYRADTVRNIDQWYQAFDVKPGGKLYLAPAERVRL
                     W"
BASE COUNT          995 a         1468 c         1527 g          838 t
ORIGIN      
        1 gacctgctgc gcatcccagc gcataggcaa tccacatgaa ccatgcggac ggcgtgcgcc
       61 acatctgtcc gggcgtgaat tcgtacgtcg tccctgctgc cttggcggcc ggagccggag
      121 tccagccttc ggggcggtag ccgacaggcg ggttcttgag caggaacgcg ccgatcatgg
      181 tcatgacgaa gaacactgcg ccgaggacga ggaacgtgtt gcgccatccg aaggtgggga
      241 tgagccacag gtttgcaagc gggccgaaga tcgccgatcc gccaccataa ccagcgacgg
      301 caagaccgac ggccaggccg cgcttgtcgg ggaaccattt ggccatgacc gggatgggtg
      361 tggcatagcc gaagccatta ccgattccga cgagcacgcc gaagcagagg atcagatacg
      421 tgaggctgtg cgtctgcgac gagagcagga atccgacggt gagcaggatc gagcccacga
      481 cggacaccat gaatggtccg agcttgtcct gcagccgtcc ggccagaacg aagctgagcg
      541 cgaacaccac gatcgcccag gtgaagacga gtgaggtatc ggcacgcttc cagccgaact
      601 ctttctccag tggtgcgaca aagacgctcc aggcgtaaag cgagccaagg gcaagattca
      661 tgaacagacc acccaatacg cgccaccacc ggttcaatgt gacttcggga gtgttaccca
      721 ttttctctgc ctccgttttt ttcgttgacg tgagattgct gacttctgcg cgaatttctt
      781 agaaatagaa gaactgcaac tgcacggtga gctcgttcgt cttgttgccg aggcgcggat
      841 cgacgcgcgt ataggcccct ttgacattga agttgtggcc gttcggcagc caggccacac
      901 cggcctgcac acgcttggtg tctttgccgt tgtcagcgct gcgcacgaag ttctgttcct
      961 cgaagcgcag gaacggctgg atcttgagct tgccgacgta gtacccggcc tggagcatca
     1021 gatcgtgctg ttgggccatg gcggggaagg tcttgttgcc atcgaagctg agcaactgca
     1081 cgtcggcgtt cagcgcgttg gtcttcgctg cattcggaag cgagaggaag agatctccgg
     1141 agtaggcctg atagtccgac tggtgatcgg tgccggcgcc gatggccacg atcttcttgt
     1201 tgcccatgta aaagccggga tagaagaagc cgccgacttc tgtgtcgtag aagttgtatt
     1261 ggaggcgggc cgtcgtgcgg agcgggttgc gcgcgcctgc cagtctcatt ccctggagca
     1321 gcgctgcccg gtattcgaga cggccgccat caaggtaacc tttggcctgg aatccggtgt
     1381 cgcggccgac ggaggactgc gttgcgccgc tctcgatgaa ggaattggag ccgtagtcga
     1441 gcgccaggag cgtgcccgcg ctgttgaggc agttccggca gagcggaacc agcatcaggc
     1501 ctgcgtcgag ggcgaaggca ttgctgccgg tcggtttcca ttcgacatag gcgtcctgcg
     1561 tcatgaaacc ggtgcccagg gctttgggag acttgccgag gttgggattg tcggattcga
     1621 agaagaacgt caggttcgga gtgacctgtc ccgcaaccag aaaacgcatg cgccggatga
     1681 agaggttgtt gccatagccg cgcgaggtcg catcctgcag cgaatccccc catgactgca
     1741 gcagtccgcc aaagcggatg ttgacgttgt cgttgacttt gatgactgcc tgcgcctgca
     1801 ccactaccgg catcaggagc agaaacgcgc cagccaggaa ggtgaggatt gacggaccgc
     1861 agatgcgaga gtttccgtgt ttttcagaca tacgatctcc ttgaatgtga aggtttcctg
     1921 tcggacgatt gcgccaggac accgcctggc cggcaatgcg taatgaatcg aaggacgccg
     1981 gaaaacgacg gcctgaggtc tgaatacttt cgattgcgcg ccatggatca cgtcagcagt
     2041 tcgtcgtcag tcggttgtgt ttttgtttga ggattcgaag ctaggccacg ccgtcagtct
     2101 ctgcggtgac tgaacgtacc cgccgcccgt gacgacggac accctcgaac gtacagccca
     2161 tcacctggcg gcgtggatga ggacggaagt tgcggtgacc acgaagacgc ttcatcccgt
     2221 tttcgaaacg tcacgagcgg tgatccggac gttcaggatg tgtgtcgcgg acccacggcg
     2281 gatggaaggg aaatggggta tgcgctgcac attcggaagg gcgctccagg ttctcagagc
     2341 aggatccccc gccgggcgcg caggcaacgc gcgcgaagcg ctacgatagg gcatgcactc
     2401 ttcaaagctc atccgcgcga ccgtgttgtt gctggcggcg ttcatcgccc tggcggcgac
     2461 accgcctccg tcccacggcc tcgatctcgc gggcatggat aagaccgtca agcccggcga
     2521 cgacttttac ggctacgcca acggcacctg ggcgaaggtc acgccgattc ctcccgaccg
     2581 ctccagctat ggcgtcttca ccatcctcga cgagaaagcg aaccagagaa cctcggatct
     2641 catccgggaa acagagaagg ccaaagctcc cgccggctcg gaagtgcgca tgatcggcga
     2701 ctactacgcc gcgttcatgg acgagaagtc catcgaggcg cgaggcctga agagtgtcca
     2761 gccggagctc gacgagatcg cggccatcgc cgataagcag gccttggcaa acgtgatggg
     2821 aagccaactg cgcgccgacg tcgatgccct caacaacacg aacttctata ccgaccggcc
     2881 cttcggcctg tgggtctctc ccgacttcga caacccgaac cggaacatcg gctacctgct
     2941 ccagggcggc ttggggatgc cggaccgcga caactacctg agcaaggaga agaacgacgt
     3001 cgcgctccag gaaaagtatc gccagcacat cgccgcggtt ctcacgctgg cacacatcgc
     3061 cgacgccgcc gggaaggccg gcagaatcta tgacctggag cgcaagatcg ccgaggcaca
     3121 tggaaaccgt accgactccg aggatgtcca caaggcaaac aaccgctggc cgttgcagca
     3181 gttcccggtc aaggcgcccg gactcgattg ggcggcctac ttcaagggtg ccgggctctc
     3241 cggccagacc gagatcatgg tgtggcagcc gtccgccgcc gtcggcattg ccgcgctggc
     3301 cgggagcgaa ccgctgtcgt tgtggaagga ctacctcacc ttccacgcca tcgaccgcgc
     3361 gtcaggcctg ctgccgaaag cgtttgcgga tgagcggttc aagttctacg gcacggcgct
     3421 gaccggtgcg ccgcagcaac gcgcccgctg gaaacgcgcc gtcagtgcca cgagcggtgc
     3481 gctcggcgac gccgtgggca agctctatgt gaagcactac tttcccccct cggcgaagaa
     3541 ggccgcgcag acgatggtga gcaacatcgt ggccgccttc agccgtcgca tcgacaacct
     3601 cgactggatg aagccggaga cgcgcacgaa ggccaaggcc aaactgagca cgctctacat
     3661 cgggatcggt tatccggaac actggcgcga ctactccggg ctggtgatca aacgcgacga
     3721 cgccctcggc aacgagcagc gctccgagtt gttcgactat cgagccgccg tggccagact
     3781 cggcaagcca gtggacaagt cgtcgtgggc gatggagccg cagaccgtga acgccgtcaa
     3841 cctgccgctg cagaacgcgc tcaatttccc ggccgccatc ctgaaccccc ctttcttcga
     3901 tgagaaggcg gatgcggtcc tcaactacgg agcgatcggc acggtcatcg gtcacgagat
     3961 cagccacagc ttcgacgacc agggaagcca gttcgacgcc gaggggcggc tgctgaactg
     4021 gtggacgaaa gaagacttcg cgcacttcag cgcggcggcc gaccgtctgg ctgcggagtt
     4081 cgatacctat gagccactgc ccggactgca cgtcaacggc aagctgacgc tgagcgagaa
     4141 catcgccgac gtggccggcc tctccgcgtc ttacgacggc tatcgccggt tctacggcga
     4201 caaggcaggt ccctcggccg caggattcac gggcgaccag cgcttcttcc tcgccttcgc
     4261 gcaggcctgg cgatcgaagc tacgccccga ggcgcttcgc aactcgctga agaccaacgg
     4321 ccacgcccca ggtcaatacc gcgccgacac cgtccgcaac atcgaccagt ggtaccaggc
     4381 gttcgacgtc aagccgggag ggaagctgta cctggcaccg gcggagaggg tgcgtttgtg
     4441 gtgaagcctg cgctacgacc gcccgccgta gtgcaggcat tcttgcctgc tcccgccggg
     4501 cgtccctgcc cggcggtggc gtggggcgga ccgagttatt ttctcacctg aagggtaggg
     4561 aaactgctgc tttcagccac tcctgagtag acagggtacg aagcgagtgt cctgacggag
     4621 tgaaccacaa tttacgatca acctggagga ggccatgaaa ccaaaatcat cgagagccag
     4681 cgggaaggca cgtagcgatg gcaagcagaa aagcacgcag aaagcggtga agtctcgtcg
     4741 agccgtaacg gcaaagagca ccaagggaag cgcgaaagcc gcagcgaatc tcgccccgtc
     4801 gttcaggccg caccggcggg ttaccgtc
//
LOCUS       DATLVW010000685         6232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_73376, whole genome shotgun sequence.
ACCESSION   DATLVW010000685 DATLVW010000000
VERSION     DATLVW010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73376"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VNN25_19885"
     CDS             complement(<1..959)
                     /locus_tag="VNN25_19885"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HXH93850.1"
                     /translation="MRRIFLLLGAALLLSRAAMAAQAADTPARTIHRAAGPITIDGDL
                     SDPGWQNALKSETWYETNPGDNTEPKVKTIGYTTYDDHFFYAAIRSWDNPADLRAQLG
                     DHDGISGNSDDFAGVILDTRNDGKSAMEFFVNVAGTQYDAAQDDFSGEDSSPDLFWDS
                     AVKKTSDGWILELRIPFSSLRYQQTSPQVWGIMMYRNMPRERRYQLFDHKLPRGSNCF
                     VCNCNKVAGFEGLPTGGHIIVAPYVTASENGVLRGNGNNSLVNRPASGDGGVDIKYTP
                     NEHTAIDGTINPDFSQIESDVAQISTNQRFAIFFPEKRPFFLER"
     gene            complement(1159..3237)
                     /locus_tag="VNN25_19890"
     CDS             complement(1159..3237)
                     /locus_tag="VNN25_19890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH93851.1"
                     /translation="MIDIREVRVLLVEDDEDDYVLTRDLLTDSRRTRFVLDWMPSYGE
                     AIAAIARGQHDVYLVDYRLGEHDGLEILREARAAGCVRPIILLTGQGDGEVDIAAMQA
                     GAADYLVKGQLDSQILERSIRYSLEQNRTLEALRESEERYALSARGANDGLWVWDLRD
                     DTVYYSDRWKEMLGFTQDEVSPSPDEWFSRVHPDDVAMLRADIDAHRHGNTPSIEHEH
                     RMRCWDGRYRWMLARGVAVRDAAGVATRVAGSQTDITERKVATDRLMHDAFHDGLTEL
                     PNRALFMDRLDNSLERSRHYSDSQFAVLFLDLDRFKVVNDSLGHALGDQLLIAVADRL
                     RATLGPTDTVARLGGDEFTILLEIDHLADAVRTAERIQENLRVPFRVGEHEIFATASI
                     GIALSATGYERSQDVLRDADIAMYRAKSRGRAVHEVFDKTMHERAVKLLKFETELRRS
                     IERDELRVYYQPVVSLETNRVVGFEALVRWQLGDELVTADEIVPIAEDTGLILSIGLW
                     VLREALRQLAAWQKVNPAMARLDMHVNLSAKQFLQPNLLEQVAAALADSGVVPQRLHL
                     EVTESVIIDNPEEAAALLERLRETGVRISLDDFGTGYSSLSSLSQFPFDTLKIDRSFI
                     STNGDRRNDEIVRTISNLARILGMDVTVEGLESADQVGRMRELGIEFAQGFFFSPPVD
                     AETAEGLVGA"
     gene            complement(3234..3695)
                     /locus_tag="VNN25_19895"
     CDS             complement(3234..3695)
                     /locus_tag="VNN25_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH93852.1"
                     /translation="MAAHAKPIVILLADDDEEDRMLTSDALIESRVVNDLRFVEDGEE
                     LLDYLHQRGRYSDPTAAPSPGLILLDLNMPRKDGREALREIKADPRLRRIPVIVLTTS
                     KAEEDIFRTYDLGANSFITKPVHFNALVEVMKEIGRYWIEIVELPNGAANR"
     gene            complement(3916..5688)
                     /locus_tag="VNN25_19900"
     CDS             complement(3916..5688)
                     /locus_tag="VNN25_19900"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93853.1"
                     /translation="MPFQFRLAAVPWVVMCVALVTLAGVIVMRQRASRVARLFSLMLF
                     LVAGWFLGYAAMFSTSSPSLAAMFGRMAIACVAFLPAAVFDFTATSIRSYVRDRVIVL
                     SAWTAAAVFAVLILATDLIIDGTRQFAWGRYPRAGSGGAIFLVYFFAVLALQMYTYAV
                     EYRKTTDERRRRRLLNLMASFGIVYLSAIDFAPMFGVDHQPAGYVPVFAFVLFAWRSI
                     RSHRFYNITAARAAREILDTMADALFVIDSEARIRVVNDAVHKLFGYTEPEILGKTID
                     TMEYIDHEGSISRTLHNMLRRAPIRDEERIFCTKNGEPIDVSIAVSSLHEREIEGGAV
                     VIARDIRERRRAHDELHAFTVKLQQSNRELEDFAYVASHDLQEPLRKIQAFGDRLRAR
                     YADTLTGEGADYLKRMQAAAARMQTLINDLLVFSRVTTKAQPFVPVDLNTVVHEVVHD
                     LEVRIHQAGAEVRIGELPMIDADPSQMHQLIQNLISNGLKFHKPDTPPRVSISGKVSH
                     DSAEIVVSDEGIGFEERYADRIFTMFERLHGRGTYEGTGIGLAICRKIAQRHGGDILA
                     QSTPGQGSTFTVTLPLVHRSGFAD"
BASE COUNT         1116 a         1875 c         2060 g         1181 t
ORIGIN      
        1 cgctcgagga agaacggccg cttctcgggg aagaagatcg cgaaacgctg attcgtggag
       61 atctgcgcta cgtccgactc gatctgcgag aagtcagggt tgatcgtgcc gtcgatggcc
      121 gtgtgctcgt tcggcgtgta cttgatgtcg acgccgccat cgccgctggc cggacggttg
      181 accaggctgt tgttaccgtt gccacgcagc acgccgttct cgcttgcggt cacgtacggc
      241 gcgacgatga tgtggccgcc ggtgggcaac ccttcgaagc ccgccacttt gttgcaattg
      301 caaacgaagc agttcgaccc acgcggaagc ttgtggtcga agagctggta gcggcgctcg
      361 cgcggcatgt tgcggtacat catgatgccc cacacctgcg ggctggtctg ctggtaacgg
      421 agcgacgaga acgggatgcg cagctcgagg atccatccat cggaggtctt cttgaccgcc
      481 gaatcccaga agagatcggg cgaggagtcc tcgccgctga agtcatcctg cgcggcgtcg
      541 tattgcgtgc cggcgacgtt cacgaagaac tccatcgccg acttcccgtc gttgcgcgtg
      601 tcgaggatga caccggcgaa gtcgtcgctg ttgccgctga tgccgtcgtg gtcgccgagc
      661 tgcgcgcgga ggtcggcggg gttgtcccac gagcggatgg ccgcgtagaa gaagtgatcg
      721 tcgtacgtcg tgtaaccgat cgtcttcact ttcggttcgg tgttgtcgcc gggattcgtc
      781 tcgtaccacg tctccgattt gagggcgttc tgccaaccgg gatcggagag atcgccgtcg
      841 atggtgatcg gaccggcagc gcggtggatg gtccgcgccg gcgtgtcggc ggcctgcgcg
      901 gccatcgccg cacgagagag cagcaacgcc gcgccaagca ataggaagat gcggcgcatt
      961 ggtgagtgag acgacaagaa gcgggcaaag ttagccttcg ttcgcatatt gcgggactga
     1021 tacgaagggg ttcgtaatag gttgcaggga aggtggcggc atttactggt tgcggtttcg
     1081 ctcgtcatga ctccctgact gcgcaaccag gtaacccgtt tatcccgcaa tcggcaattc
     1141 aacaaccgac cagcgaactc acgcccccac caacccctcc gccgtctctg cgtctaccgg
     1201 tggcgagaag aagaagccct gcgcgaactc gattccgagc tcgcgcatgc ggcctacctg
     1261 gtcggcgctc tccagtcctt ccacggtcac gtccatgccg aggatgcggg cgaggttgct
     1321 gatggtgcgg acgatctcgt cgttgcggcg atctccgttc gtggagatga acgagcggtc
     1381 gatcttgagc gtgtcgaacg gaaactggct gagtgagctg agcgatgagt atccggtgcc
     1441 gaagtcgtcg agactgatcc gcacgccagt ctccctcagc cgctccagca gcgccgccgc
     1501 ctcctcggga ttgtcgatga tgacgctctc cgtcacctcg aggtgcagcc gctgcgggac
     1561 gacaccgctg tccgcgagcg ccgccgccac ctgctcgagc agattcggct gcagaaactg
     1621 cttcgccgaa aggttcacgt gcatgtccag cctggccatt gccggattga ctttctgcca
     1681 ggccgcgagc tgccgcagtg cctcgcgcag cacccacagg ccgatcgaca ggatgagccc
     1741 cgtgtcctcg gcgatcggga caatctcgtc cgcggtcacg agctcgtcgc cgagttgcca
     1801 ccgcacgagc gcttcgaaac cgaccactcg gtttgtctcc agcgacacca caggctggta
     1861 gtagacgcgc aattcgtcgc gctcgatcga tcgccgcagc tcggtctcga acttcagcag
     1921 tttgacggcg cgctcgtgca tcgtcttgtc gaagacttcg tgaaccgcgc ggccgcgcga
     1981 cttggcccgg tacatggcga tgtcggcgtc gcgcagcacg tcctgcgagc gctcgtagcc
     2041 ggtggcactg agagcgatgc cgatgctggc cgtcgcgaag atctcgtgct cgccgacccg
     2101 aaacggaacg cgaaggtttt cctggatgcg ctcggcggtg cgcacggcgt cggcgagatg
     2161 atcgatctcg aggaggatcg tgaattcgtc gccgccgagg cgcgccacgg tatccgttgg
     2221 tccgagtgtt gcgcgcaaac ggtcggcaac agcgatgagg agttgatcgc cgagagcgtg
     2281 gccgaggctg tcgttcacga ctttgaagcg atcgagatcg aggaagagga cagcgaactg
     2341 gctgtcgctg tagtgtcgcg agcgctccag cgagttgtcg agacgatcca tgaaaagcgc
     2401 gcggttggga agctcggtca gaccgtcgtg aaaggcatcg tgcatcagcc ggtcggtggc
     2461 gaccttccgc tccgtgatgt cggtctgcga cccagccacg cgcgtcgcca cgcccgccgc
     2521 atctcgcacc gccactcctc gggccagcat ccaccgatac cgcccgtccc agcatcgcat
     2581 gcggtgctca tgctcgatgc tcggcgtgtt gccgtgacgg tgcgcgtcga tgtcggcgcg
     2641 caacatcgcc acatcgtcgg gatgaacgcg cgagaaccac tcatcgggcg acgggctgac
     2701 ctcgtcctgc gtgaagccga gcatctcttt ccagcggtcg gagtagtaga ccgtgtcgtc
     2761 gcggaggtcc cacacccaca aaccgtcgtt cgcgccgcgt gcggagagtg cgtagcgctc
     2821 ctcgctttcg cgcagcgctt cgagcgtgcg gttctgttcg agcgagtaac ggatcgatcg
     2881 ctccagaatc tgtgaatcga gctgcccttt gacgaggtag tcggcggcgc cggcctgcat
     2941 ggccgcgatg tcgacttcgc cgtctccctg gccggtcagc agaatgatgg gccgcacgca
     3001 gccggcagca cgcgcctcgc gcaggatctc caggccgtcg tgctcgccga ggcggtaatc
     3061 gacgagataa acgtcgtgct gcccgcgggc gattgccgct atcgcttcgc catacgacgg
     3121 catccagtcg agaacgaagc gtgtccggcg gctgtcggtg aggagatcgc gggtgagtac
     3181 gtagtcatcc tcgtcgtcct caaccaacag cacccggact tcccggatgt cgatcaacgg
     3241 tttgcggctc cgttcggaag ctcgacgatc tcgatccagt agcggccgat ctctttcatc
     3301 acttcgacga gcgcgttgaa gtggaccggc ttggtgatga atgaattcgc acctaggtcg
     3361 taggtgcgga aaatgtcttc ttcggccttc gacgtggtca ggacgatcac cgggatgcgg
     3421 cgaagacgcg gatcggcctt gatctcccga agcgcttccc gtccatcctt gcgcggcatg
     3481 ttcaggtcga gcaggatcag accaggcgac ggcgcggcgg tgggatcgga gtagcggcct
     3541 cgctggtgaa ggtagtcgag aagctcttcg ccatcttcga cgaagcgaag gtcgttgacg
     3601 acgcggctct cgatgagcgc gtcagaagtg agcatccggt cttcctcgtc atcatcagcg
     3661 aggagaatga cgatcggttt cgcgtgagcg gccatggtca gtagattact ggattcgtgg
     3721 atcgggggac agtggagagt tgacaatgga taattgacaa tggacaacgg gcaggccggg
     3781 gttctgtttc acgcggagac gcggagatcg cggtgggtta tctccgtgtg ctccgcgcct
     3841 ccgcgtgagc gcctttgatt ctcgggcaag cgttgagcga cggatcgttg tcgattgtcc
     3901 cttctcaatt gtcaactaat ccgcgaaccc gctccgatgc acgagcggca gcgtgacagt
     3961 gaacgtcgac ccctggccgg gcgtgctttg ggcgaggatg tcgccgccgt gacgttgggc
     4021 gatcttgcgg cagatggcca ggccgatgcc ggtgccttcg tacgtgccgc gcccgtgcag
     4081 gcgctcgaac atcgtgaaaa tgcgatcggc atagcgctcc tcgaagccga tgccttcgtc
     4141 cgacacgacg atctccgcgg aatcgtgtga gacctttccg gagatggaca cccgaggggg
     4201 cgtgtccggt ttgtgaaact tgagcccgtt gctgatgagg ttctggatga gctggtgcat
     4261 ctgcgacgga tcggcgtcga tcatcggcag ctctccgatc ctcacctcgg cgcccgcctg
     4321 atggatgcgc acctcgagat cgtgaaccac ctcgtgcacg accgtattga gatccaccgg
     4381 cacgaacggc tgcgccttcg tcgtcacccg tgagaagacg agcagatcgt tgattagcgt
     4441 ctgcatgcgc gccgccgcgg cctgcattcg tttgaggtag tcggctccct cgccggtcag
     4501 cgtatctgca tagcgcgccc gcaaccggtc gccgaacgcc tggatcttcc gcaacggttc
     4561 ctgcagatcg tgcgacgcga cgtaggcgaa gtcctcgagc tcgcgattgc tctgctgcag
     4621 cttgaccgta aaggcgtgca actcgtcgtg cgcccgcctg cgctcgcgga tgtcgcgcgc
     4681 gatgacgacc gcgcctcctt cgatctcccg ctcgtgcagc gaggagaccg cgatgctgac
     4741 gtcgatcggc tcgccgttct tcgtgcagaa gatgcgctct tcgtcgcgga tcggcgcccg
     4801 gcgcagcatg ttatgcagcg tccgcgagat gctcccttcg tggtcgatgt actccatcgt
     4861 gtcgatggtc ttgccgagga tctcgggctc cgtgtaaccg aagagcttgt gcacggcatc
     4921 gttgacgacg cggatccgcg cttcgctgtc gatgacgaaa agagcgtcgg ccatggtgtc
     4981 gagaatctcg cgcgcggccc gcgcggcggt gatgttgtag aagcgatgac tgcggataga
     5041 gcgccaggcg aacagaacga aagcgaagac cggcacgtag ccagccggtt gatgatcgac
     5101 gccgaacatc ggcgcgaaat cgatggccga cagatagacg atgccgaagc tggccatgag
     5161 gttgaggagc cggcggcgcc tccgctcgtc ggtggtcttt cggtattcga ccgcgtacgt
     5221 gtacatctgc aacgcgagaa cggcgaagaa gtagacgagg aagatcgctc cgcccgagcc
     5281 cgccctcgga tacctccccc acgcaaactg ccgggtgccg tcgatgatca gatccgtagc
     5341 cagaatgagg accgcgaaca cagcggcagc ggtccaggca ctcaaaacga tgacgcggtc
     5401 gcgaacgtag gagcggatcg aggtcgccgt gaagtcgaag accgccgcgg gaagaaaggc
     5461 cacgcaagcg atcgccatac gcccgaacat cgccgccagg gatggggacg acgtcgagaa
     5521 catcgccgcg tacccgagaa accaccccgc gacgagaaac agcatcagcg aaaagagccg
     5581 cgccacccgc gaagcccgct gccgcatcac gatgacgccc gcaagcgtga cgagcgccac
     5641 gcacatcacc acccacggaa ccgcagccag ccggaattgg aaaggcactg gggcattgta
     5701 gggtgtggga ggtggggggt ggggagtggg aagggcgggt gtcggtgttc gggtgtcggt
     5761 gttcggtgtt cgggggtcgg tgttcggagg tcgggggtcg gggtcggagc caattctcgg
     5821 gtagttgatt gtggattctc gggtctggct tgtcggagtc acgaaatcga gcttggtgtc
     5881 atcctggtgt catcctgaga ctgccgcagc cgcccgcgtg aatcgagatg tttgcgctgt
     5941 gcgtgcgcgg cggcgtagga cgtcgaagga tctcaaactt cgcagatcgt gttcgcccag
     6001 cgcgagcgat cgtgatcgaa gacgcacgaa cgggatcttc gtattttgag atccttcgac
     6061 cgtcctacgc cgccgatgcc tgccgggtgc gagcgtcgtg aaacttgcgg cggcttcggc
     6121 ggctcaggat gacacatcac gttttgagcg ccatccaaag gccatcatgg ctctcgagct
     6181 acccgcaaac accggacaag gttgccgctg ttcgggggtc gggggtcgga gc
//
LOCUS       DATLVW010000686         9663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_155377, whole genome shotgun sequence.
ACCESSION   DATLVW010000686 DATLVW010000000
VERSION     DATLVW010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155377"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VNN25_19905"
     CDS             <1..918
                     /locus_tag="VNN25_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HXH93854.1"
                     /translation="PYEATTSYGGGTSNGPSAIFEASKQVDLFDRDTGRPYEAGIAML
                     DIPKKIERCNRKAKEIHAAVIEKGGPVDSATRKAVAEVNAISELVNEWVHEQTRALLD
                     AGKMAVTLGGDHSVPFGAMQAYAETYPGIGILHLDAHADLRDSYEGFTWSHASIFHNV
                     MTKIGGVAKLVQFGVRDLSNAESAMIENSGGRIVTFFDSDLAARKDEGATWAEIADEI
                     VGALPNDVYLSWDIDGLDPTLCPSTGTPVPGGLSWNEAIGVLRAIVRAKKRIVGLDLC
                     EVAPGQTEWDANVGARLLYKMIGFALMTQ"
     gene            complement(1043..2092)
                     /locus_tag="VNN25_19910"
     CDS             complement(1043..2092)
                     /locus_tag="VNN25_19910"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXH93855.1"
                     /translation="MTEKHHVAIIGAGPIGLELAVAFKQFGIDYVQLDAGQIGSTIEW
                     YPIQMLFHSSSERLGLAGVPIQTSTQQKITREEYLAYLRSIVLQFGLQIRSYERVMRA
                     TRLPGGEFELMTRAVDGEHRYQVDHVVLAIGAFHAPRMLDIPGEEMAHVSHYFHDPHT
                     YFGKKLLIVGGRNSSVEAGVRCQRAGADVTLSYRRDDFDPKVVKFWLLPEVRAQIRDG
                     LIRYLPRTTPTEIGNGTALLAPTDDRGALTPDAVPTEIDADFVLLLTGYRQDSTLFDQ
                     LGVELRGDDRQPVHDPETMETNVPGVFVAGTAVAGTPPRKVTVIVETCHVHVPRILAA
                     IQGKRVALPDGAVDD"
     gene            complement(2207..2677)
                     /locus_tag="VNN25_19915"
     CDS             complement(2207..2677)
                     /locus_tag="VNN25_19915"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HXH93856.1"
                     /translation="MSLARLMQRFKGGVCTDEGPPQQQDPLRLATAAVLLDIGYADGT
                     LSSEEDEDLLSYLKRAFALDDTMAQELVKAADEIRTQSIDHYALTNFIRTSAPLNDRI
                     EIVKTMWRIAYADGRLTDYENYLVRKLADLLGLEHHVMIDAKVSVLRELGLATS"
     gene            2777..3256
                     /locus_tag="VNN25_19920"
     CDS             2777..3256
                     /locus_tag="VNN25_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93857.1"
                     /translation="MPRIDINQLDDHARMWLFAISPALNETQSKQFLDRVDRFLDGWA
                     AHDVPVTSARELLYGSFLALAVDERSETSGCSIDRMFGLLKQFEQELGVSILDANRVF
                     VRHADGRIEALSRAEFRDRGDLHTTIFDTTADRLSEIRTGHWIRKAEEAWPGKLLTT"
     gene            complement(3428..5230)
                     /gene="treZ"
                     /locus_tag="VNN25_19925"
     CDS             complement(3428..5230)
                     /gene="treZ"
                     /locus_tag="VNN25_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXH93858.1"
                     /translation="MANQKTTRKRAVGAEIIDGGVDFRLWAPDRKNVSVVINGRAIPL
                     DREADGHFRGTVADARAGTRYRFRLDDDDKLYPDPASRFQPNGPHGDSEVVDPAAYRW
                     NDGAWAGVDKRNLVIYEMHIGTFTAEGTWRTAIAHLGALRDIGINLLEVMPVHEFPGR
                     FGWGYDGVDLWAPSHLYGRPDDFRVFVDAAHAHGLGVILDVVYNHFGPDGCYLRQFTK
                     NYFTNKYVNDWGESINFDGDGSAGVRELVSENAAMWIGEFHLDGLRLDATQSINDASD
                     EHVLALIARRARAAATDRSIFLVAENEPQDVALIDDYGLDALWNDDWHHAAMVAATGR
                     REAYYTDYFGTPQEFVSMAQFGFLYQGQYYSWQKEPRGTSSLHLSPRQLVCFTQNHDQ
                     IANSGRGERLQQLTSPGRFRALTALLLLGPNTPMLFQGQEFGAATPFLYFADHNPELA
                     KAVGKGRREFLEQFPSLLKMKGQFAPPEAEETFRRCKLDDRERVANVEMIALHRDLIR
                     LRREMSGTVRGAVLAEEAFVLRFDNRLLIVNLGREVHLDVVPEPLLAPPTKQKWELLW
                     SSESPDYGGTGTPAIEIGGRWCLSAQAAVLLAAT"
     gene            5412..6797
                     /locus_tag="VNN25_19930"
     CDS             5412..6797
                     /locus_tag="VNN25_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HXH93859.1"
                     /translation="MNLLHLFDLSLIVRRDQIALEWEGAQFTFGEIENRSNRLGHALR
                     ARGLIKGDRLAVYLANRIELIDLYLACVKLGVIFVPINILYRDREIAHITGDAEPRFV
                     IREEDLPALMMEAENASSERPAEDLDGDTPAAIIYTSGTTGASKGAVLTHNNFAANAV
                     NLLTMWQISDRDRFLLALPLFHIHALGNGLHCWLASGCRMRLLERFDHRTALNEFNEF
                     QPTLFFGVPTMYMRLLETPPEAAREIGARMRLFVSGSAPLPANLFDRFRELFGRAILE
                     RYGMTETFMTLSNPYIGERRAGTVGFPLPGISVRIEDGELLVRGPNVFAGYWRRPDAT
                     AAAFTDGRFRTGDLAARSDDGYYTLLGRKSDLIISGGFNIYPREIEEMLLEQPGVAEA
                     AVVGEPDELRGEVPVAFIVAASESALDIDSLERLCREQLASFKVPRQFVRVDSIPRTA
                     LGKVQKHLLPR"
     gene            6849..7571
                     /locus_tag="VNN25_19935"
     CDS             6849..7571
                     /locus_tag="VNN25_19935"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HXH93860.1"
                     /translation="MCLIAIAVHTSAHFPIVIAANRDEFYDRPTRPLHRWDDAKNIAG
                     GRDLRVGGSWLAITQEGRFAAVTNIRGINVANPRSRGELVGNFVRSDLDPLDYAQQIK
                     PNEYAGFHLIAGDRARVAHVASDVPATLLPVGIFAVSNAPALIDWPKIVLAREAMAGV
                     LDHPPGQLVDELMRFLTTPRGGPIEQEVFVALPDRGYGTRSSTVIVMREDEIAIREMS
                     HPEGAVVALNLSREGKRSGADK"
     gene            7750..7914
                     /locus_tag="VNN25_19940"
     CDS             7750..7914
                     /locus_tag="VNN25_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93861.1"
                     /translation="MVETRLEASDNDVQRAWAVEIERRSRDAVKERGEDWRVVLDEIR
                     GGLLQRSGSR"
     gene            complement(7985..9304)
                     /gene="pepQ"
                     /locus_tag="VNN25_19945"
     CDS             complement(7985..9304)
                     /gene="pepQ"
                     /locus_tag="VNN25_19945"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HXH93862.1"
                     /translation="MNDRFAALYPQHLETVKARHDKALTEAGFDHIIIFGGEIHIQFL
                     DDSFYPFKVNPHFKSWVPIIDNPHCFIVYTPGKTPLLVYYQPVDYWYKPAGAPQGYWT
                     DHFDVRVIGTAEAAKEFFPTTGRAAFVGEQSDLHGGEANPEGLLNALHFERSWKTDYE
                     IACLTEANVRGVRGHRAAARAFREGKSEFEIHIDYLLHSSQAEEELPYGNIIALNENA
                     SVLHYLNHVREQPGDLRYSFLIDAGAQCNGYASDITRAYARDEQGEFARMIEAMDAMQ
                     QRLCTMAKPGVNYIDIHMAAHAEVAKILFDFGFVRDVDAEGIVEKKISSAFFPHGVGH
                     FLGLQVHDVAGFHGNRAGCIIAKPEGHPYLRLTRIIDPRMVFTIEPGLYFIESLLSDL
                     RKSENAKYINWSKVDDFRKFGGIRIEDDVVVTDEGHINLTRDAFGGS"
     gene            complement(9353..>9663)
                     /locus_tag="VNN25_19950"
     CDS             complement(9353..>9663)
                     /locus_tag="VNN25_19950"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93863.1"
                     /translation="FSQGGLMAIDAGFRTKQKVAGIVVMSGAIYEADMPDFRAHRDLS
                     VLVIHGTQDDMVKVLYARRTRHILEEHGVIPEYHEFPMGHHVTQEPMLVVREFLKRVL
                     "
BASE COUNT         1803 a         3014 c         2999 g         1847 t
ORIGIN      
        1 ccgtacgagg cgaccacatc gtacggcggc ggcacttcga acgggccgag cgcgatcttc
       61 gaggcgtcga agcaggtcga tctcttcgac cgcgataccg gccgccccta cgaagccggg
      121 atcgccatgc tcgacatccc gaagaagatc gagcgctgca atcggaaggc gaaagagatc
      181 catgccgcag tcatcgaaaa gggcggcccg gtcgatagcg cgacgcgaaa agcggtggcc
      241 gaggtgaatg cgatctcgga gctggtaaac gagtgggttc acgagcagac gcgcgcactc
      301 ctcgacgccg gcaagatggc cgtcaccctc ggcggagatc actccgtccc gtttggcgcc
      361 atgcaggcct acgccgaaac ctatcctggc atcggcatcc ttcatctcga tgcccatgca
      421 gatctgcgcg actcctacga aggcttcacc tggtctcacg cctccatctt tcacaacgtg
      481 atgacgaaga tcggcggtgt cgcgaagctt gtgcagttcg gcgtgcgcga tctcagcaat
      541 gccgagtcag cgatgatcga gaattcgggt gggcgcatcg tgacgttctt cgattccgat
      601 ctcgctgcgc gcaaggacga aggggcgacg tgggcggaga tcgctgacga gatcgtcggt
      661 gcgctgccga atgacgtcta cctctcctgg gacatcgacg gccttgatcc gacgctctgc
      721 ccgtcgaccg gaacgccggt gcctggtggt ctctcttgga acgaagcgat cggagttctg
      781 cgcgcgatcg tccgcgcaaa gaaacgcatc gtcggcctcg acctctgcga agtcgcgcct
      841 ggccagacgg agtgggacgc gaatgtcggc gcgcgattgc tgtacaagat gatcgggttc
      901 gcgctgatga cgcagtaagg ccttatggtg cacagacagg agtgtctgtg ccacatcaca
      961 tactccctgt ggcacgagca ctcttgtcgt tgtacatccg gaatcggtgt ggcacagaca
     1021 ctcttgtctg tgctcccccg atctaatcgt ccaccgcccc atccggcaac gccacgcgct
     1081 ttccctgaat cgcggccagg attcgcggta cgtgcacgtg gcaggtctcg acgatcaccg
     1141 tcaccttgcg cgggggtgtt cccgcgaccg cggtgccggc gacgaagacg cctggcacgt
     1201 tcgtctccat cgtctcggga tcgtgaacag gctggcgatc gtcgccgcgc aactcgacgc
     1261 cgagttgatc gaagagagtc gaatcctggc gatagccggt cagcagcagc acgaaatcgg
     1321 cgtcgatctc cgtcggcacg gcgtccgggg tgagggcgcc gcgatcgtcg gtcggtgcga
     1381 gaagtgcggt cccattgccg atctcggtag gagttgtgcg aggaaggtag cgaatgagcc
     1441 cgtcgcgaat ctgcgcccgc acctcgggca gcagccagaa cttcacgacc ttcggatcga
     1501 agtcgtcgcg gcgatagctg agcgtcacat ccgcgccggc gcgctggcag cggacccccg
     1561 cttcgaccga tgagttgcgt ccgccgacga tgagcagctt cttgccgaag tacgtgtgtg
     1621 gatcgtgaaa gtagtggctg acgtgcgcca tctcttcgcc cgggatgtcc agcattcgcg
     1681 gcgcgtgaaa ggctccgatg gcgaggacga cgtgatcgac ctgatagcga tgctcgccgt
     1741 ccacggcgcg cgtcatcaac tcgaattcac cgcccggcag tcgcgtcgcg cgcatgacgc
     1801 gctcgtacga acgaatctgc aaaccaaact gcagcacgat cgatcggaga taggcgaggt
     1861 actcctcccg cgtgatcttc tgctgcgtcg aagtctgaat cggaacgccc gccagtccca
     1921 accgctcgga gctggagtgg aagagcatct gaatcggata ccactcgatc gtcgaaccga
     1981 tctgtccagc gtcgagttgg acatagtcga tcccgaactg cttgaacgcc accgccagct
     2041 cgagcccgat cggacccgca ccgatgatgg caacgtgatg tttttcggtc atccgcggga
     2101 gtctatacgt agaccgaacg ggcgtcgggg gtcgggagtc ggagccgagc ggtcacgagc
     2161 cagagcgctc tccgactccc gactcccgaa acgcgacccc cgcaaatcag ctcgtcgcca
     2221 atcccagctc ccgcaggacg ctcacctttg catcgatcat cacgtgatgc tcgagtccga
     2281 ggaggtcggc cagtttgcgg acgaggtagt tctcgtagtc tgtgagccgg ccgtccgcgt
     2341 acgcgatccg ccacatcgtc ttcacgatct cgatgcggtc gttgagcgga gcgctggtgc
     2401 ggatgaagtt ggtgagcgcg tagtggtcga tcgattgcgt gcggatctcg tcggccgctt
     2461 tgacgagctc ctgcgccatc gtgtcatcga gagcgaaggc gcgtttgagg tagctgagga
     2521 gatcctcatc ctcctcggag ctgagtgtgc cgtcggcgta accaatatcg agcagcacag
     2581 ctgccgtggc gaggcgcagc gggtcctgct gttgcggcgg cccctcgtcg gtacagacgc
     2641 cacctttgaa acgttgcatc aatcttgcga ggctcatgag agtgaatgat gaaggatcaa
     2701 tgatgaagga tgaaggagga acgatgaata atgcagccgg ttacccgttc atcattcatc
     2761 cttccaagga gacatcgtgc cacgaattga catcaaccaa ctcgacgacc acgcccgcat
     2821 gtggctgttc gccatctcgc ctgcgttgaa cgaaacacag agcaaacagt ttctcgaccg
     2881 cgtcgaccgc tttctcgatg ggtgggcggc gcacgacgtt ccggtcacgt ccgcgcgcga
     2941 gttgctctac ggcagtttcc tcgcccttgc cgtcgacgag cgcagcgaga cctcggggtg
     3001 ctcaatcgac aggatgttcg gccttctcaa gcaattcgaa caggagctcg gcgtctcgat
     3061 cctcgacgcc aaccgcgtct tcgtccgtca cgccgacggc cgcatcgaag cgctgagccg
     3121 agcggagttc cgcgatcgtg gcgacctgca cacgacgatc ttcgacacca ccgccgaccg
     3181 cctctccgag atccgcaccg gccactggat ccgcaaagcc gaggaagcgt ggcccggcaa
     3241 attgctgacc acgtagtgca ggcattcttg cctgctaccg ccgggcgtcc ctgcccggcg
     3301 gtggttttgg cgggattgca gtttccgcgc tgacagtgct gtctttgttg tcttcgtctc
     3361 tgtcgatcat tcccgcccat cagcgccggg cgaggacgcc cgccgcatgc agacaagaat
     3421 gtctgcacta cgttgcggcc agtagtacgg ccgcttgcgc cgataagcac catctcccac
     3481 cgatctcgat tgccggcgtt cctgtgccgc cgtagtccgg cgattcgctc gaccagagta
     3541 attcccactt ttgcttcgtt ggcggcgcga gaagcggttc ggggacgacg tcgaggtgca
     3601 cctcgcgccc gaggttgacg atcaggaggc gattgtcgaa gcggagcacg aacgcctcct
     3661 cggcgagcac ggcaccgcgc accgtgccgc tcatctcgcg gcggagccgt ataaggtcgc
     3721 gatgaagagc aatcatctcc acgttcgcga cccgctcacg atcatcgagt ttgcatcgcc
     3781 tgaacgtctc ctcggcctcg ggtggcgcga actggccctt catcttgagc agggaaggga
     3841 actgctccag aaactcgcgg cgccctttgc ccacggcctt cgccagctca ggattgtgat
     3901 cggcaaagta gaggaagggg gtggctgcgc cgaactcctg cccctgaaac aacatcggcg
     3961 tgtttggtcc gaggaggagc agtgcggtca gcgcgcgaaa ccggccggga ctcgtcagtt
     4021 gctgcagccg ctcgccgcgt cccgagttgg cgatctggtc gtgattctgc gtgaagcaga
     4081 cgagttgcct cggcgatagg tgtagcgacg acgtgccgcg cggctccttc tgccatgagt
     4141 agtactgtcc ctgatagaga aagccgaact gcgccatcga gacgaattcc tgcggcgtgc
     4201 cgaagtagtc ggtgtagtaa gcctcgcgcc gtcccgttgc ggcgaccatt gccgcgtgat
     4261 gccagtcgtc gttccagagc gcatcgaggc cgtagtcgtc gatgagcgcg acatcctgcg
     4321 gctcattctc cgcgacgaga aagattgatc gatcggtcgc agccgcccgt gcacgccgcg
     4381 cgatcagcgc gagaacatgc tcatcggacg catcgttgat cgactgcgtg gcgtcgaggc
     4441 gcaatccgtc gagatgaaac tcgccgatcc acatcgccgc gttctcggag acgagctcgc
     4501 gcacgccggc ggaaccatcg ccgtcgaagt tgatcgactc gccccagtcg ttcacgtact
     4561 tgttcgtgaa gtagttcttc gtgaattgtc ggagatagca gccgtcgggc ccgaagtggt
     4621 tgtagacgac gtcgagaatg acgccgaggc cgtgagcgtg agcggcgtcg acaaaaacgc
     4681 ggaagtcatc ggggcggccg tacaaatgcg acggcgccca gaggtcgacg ccgtcatatc
     4741 cccagccgaa gcgtccaggg aactcgtgaa cgggcataac ttcgagaagg ttgatgccga
     4801 tgtcgcgaag cgcgccgagg tgggcgatgg cagtgcgcca ggtaccttcg gcggtgaagg
     4861 tgccgatgtg catctcgtag atgacgagat tgcgcttgtc gacaccggcc cacgcgccgt
     4921 cgttccatcg atacgcggca ggatcgacga cttcggagtc gccatgtggg ccgttgggct
     4981 gaaagcgtga ggcaggatcg ggatagagct tgtcgtcgtc atcgaggcga aatcgataac
     5041 gggtgcctgc gcgcgcgtca gccacggttc ctcggaaatg gccgtctgcc tcgcggtcga
     5101 gaggaattgc tcgaccgttg atgacgacgc tgacattctt gcggtcaggt gcccagaggc
     5161 ggaagtcgac gccaccgtcg atgatctccg cgccgacggc gcgcttgcgt gttgtcttct
     5221 gatttgccac tgcgctgatt tcgagtgcaa gcagcgtgcg atggggtttc ggggtgtggg
     5281 gagtggggcg tagggtgtgg gagtttgatt aacgtttggg cgtacggcgc aggcatcact
     5341 cggctcaggc tcctcccacc ccccccacac cccactcccc acaccccacc acgcaatatc
     5401 atcgtgccgc catgaacctt cttcacctct tcgatctctc gcttatcgtc cggcgagacc
     5461 agatcgcgtt ggagtgggaa ggtgcgcagt tcacattcgg cgagatcgag aatcgcagca
     5521 accgtctcgg ccatgcactt cgcgcccgcg ggctgattaa gggcgaccgt ctcgccgtct
     5581 acctcgccaa tcgcatcgag ctgatcgatc tctacctagc ctgcgtcaag ctgggcgtga
     5641 tcttcgttcc gatcaacatt ctctaccgcg accgcgagat cgcgcacatc accggcgacg
     5701 cggagccgcg attcgtgatc cgcgaggagg acttaccggc gctgatgatg gaagcagaga
     5761 acgcgagcag cgagcggccg gccgaagatc tcgacggtga cacccccgcc gcgatcatct
     5821 acacgtccgg caccaccggc gcgtcgaagg gcgccgtgct gacacacaac aacttcgccg
     5881 ccaatgccgt caacttgttg acgatgtggc agatcagcga ccgcgaccgc ttcctcctcg
     5941 cgctcccgct ctttcacatc cacgccctcg gcaacggtct gcactgctgg ctggccagtg
     6001 gctgccggat gcgtttgctg gagcgtttcg accaccgaac agcgctcaat gaattcaacg
     6061 agttccagcc gacactcttc ttcggtgtgc cgacgatgta tatgcgcttg ctcgagacgc
     6121 cgcctgaggc cgcacgcgag atcggggcgc gaatgcgcct gttcgtatcc ggctccgctc
     6181 cgttgccggc gaacctgttc gaccgattcc gcgagctctt cggccgcgcc atcctcgagc
     6241 gctacggcat gaccgagacc ttcatgacgc tgagcaatcc gtacatcggt gagcgccgcg
     6301 ccgggaccgt cggctttccg ctgcccggca tctccgtgcg catcgaagat ggcgagctac
     6361 tcgttcgtgg tccgaatgtc ttcgccggct actggcgccg tcccgatgcc accgccgccg
     6421 cgttcacaga cggccggttc cgcaccggcg acctggcggc gcgttccgat gacggctact
     6481 acacgctgct cggccgcaag tccgatctca tcatctccgg cggcttcaac atctatccgc
     6541 gcgagatcga agagatgctg ctcgaacaac ccggagtggc cgaggccgcg gtggtaggag
     6601 agccggacga actgcgcgga gaagtcccgg tcgcgttcat cgttgctgcg tccgagtccg
     6661 cactcgacat cgattccctt gagcgcctct gccgcgaaca actggcatcc ttcaaggtcc
     6721 ctcgccaatt cgtgcgcgtc gattcgatcc cccgcaccgc gcttggcaaa gtgcagaagc
     6781 atcttctgcc ccgataaacg acgtagcgcc ggcggcgacc ttgagaccgg cattactatc
     6841 gaacatccat gtgcctcatc gccatcgccg tccacacctc cgcgcacttc ccgatcgtca
     6901 tcgcagcgaa ccgcgacgag ttctacgacc gccccacgcg accgcttcat cgttgggatg
     6961 acgctaagaa catcgccggc ggccgtgatc tccgcgtcgg cggaagctgg ctcgcaatca
     7021 cgcaggaagg ccgcttcgcc gccgtgacga acatccgcgg catcaatgtc gccaatccac
     7081 gctcgcgcgg agaactggtt gggaacttcg tgcgatcgga cctcgatccg ctcgactacg
     7141 cgcagcagat caagccgaat gaatacgccg gattccatct catcgccggc gatcgagccc
     7201 gcgtcgcgca cgtcgccagc gatgtgcccg cgaccttgct gcccgtcggg atcttcgccg
     7261 tcagcaacgc acctgcgttg atcgactggc cgaagattgt cctcgcgcgc gaggcgatgg
     7321 ccggtgtgct cgaccatccg cctggtcaac ttgttgatga actgatgcgc ttcctgacca
     7381 cgccgcgcgg tgggccgatc gaacaggaag tcttcgtcgc gctgcccgac cgcggttatg
     7441 gcactcgctc gtcaaccgtg atcgtgatga gggaggacga gatcgcgata cgggagatga
     7501 gtcatccgga aggcgccgtg gttgcgctca acctgagtag agaaggaaaa aggagcggtg
     7561 ccgacaaatg agataggcgc tcccgataac gggatctcgc tctgagagct gtcctgacgc
     7621 cccggcacaa agatctcgaa gccggaactt ggacccgtgg tcaccgctcc gaggcgatga
     7681 tctcgcattc gagcgcgtcg ggcaagtggg attgagtctc atttatgctc ccgaggtaaa
     7741 ctccttcaca tggtcgagac gaggttggag gcatcagaca acgatgtgca gcgcgcctgg
     7801 gccgttgaga tcgagcggcg gtcgcgcgat gcggtgaagg aacggggcga ggactggcgc
     7861 gtcgtgctcg atgagattcg cggcggctta cttcagcgtt caggttctcg ttgaaaagcg
     7921 ctcacgaaga cgcgaagccg cgaagaaacc cttcgcgtac tccgcgtctc cgcgtgaaac
     7981 gcttctaaga acccccaaac gcatcgcgcg tcagattgat atgcccctca tccgtcacca
     8041 cgacgtcatc ctcgattcgg atcccgccga acttgcggaa gtcatccacc ttgctccagt
     8101 taatgtactt cgcgttctcg ctcttgcgca gatcggacag cagcgactcg atgaagtaga
     8161 ggccgggctc gatcgtgaag accatgcgtg gatcgatgat acgcgtgagg cgcaggtagg
     8221 gatggccttc cggcttggcg atgatgcagc cggcgcgatt tccgtggaag ccggctacgt
     8281 cgtgaacttg taatccgagg aagtgaccga cgccgtgggg gaagaaggcg gagctgatct
     8341 ttttctcgac gatgccctcc gcatcgacat cgcgcacaaa gccgaagtcg aaaagaatct
     8401 tcgccacctc ggcatgcgcg gccatgtgga tgtcgatgta gttcacgccc ggtttcgcca
     8461 tcgtgcagag gcgctgctgc atcgcgtcca tcgcctcgat catccgcgcg aactcgccct
     8521 gttcgtcgcg cgcgtacgct ctggtgatgt ccgacgcgta accgttgcat tgggcgccgg
     8581 cgtcgatcag gaaggagtag cgtagatcgc ccggctgctc gcgcacgtgg ttgaggtaat
     8641 gaagcaccga ggcgttttcg ttgagggcga tgatgttgcc gtaggggagt tcctcctcgg
     8701 cctggctgga gtgcaggaga tagtcgatgt ggatctcgaa ctccgacttc ccctcgcgaa
     8761 acgcacgcgc cgccgcgcga tgtccgcgca cgccgcgcac gttggcctcg gtgaggcagg
     8821 cgatctcgta atcggtcttc cacgagcgct cgaagtgcag cgcgttcagc agcccctcgg
     8881 gattcgcttc accgccatgc agatcgctct gctcaccgac gaatgctgcg cgtcccgtcg
     8941 tcggaaagaa ctctttggcc gcttccgcgg tccctatgac gcgcacgtcg aagtgatcgg
     9001 tccagtatcc ctgtggcgcg cccgccggct tgtaccagta atcgaccggc tggtagtaga
     9061 cgagcagcgg ggtcttgccc ggcgtgtaga cgatgaagca gtgcgggttg tcgatgatcg
     9121 gcacccacga cttgaaatgc ggattgacct tgaacggata gaacgagtcg tcgagaaatt
     9181 gaatatggat ctcgccgcca aagatgatga tgtgatcgaa ccccgcctcg gtcagcgctt
     9241 tgtcgtggcg ggccttcacg gtctcaagat gctgcggata gagggcggcg aagcggtcgt
     9301 tcatgcgcgg agaatataac gataggcgtt gattccggcc cgtgcgtctg cgttacaaaa
     9361 cacgcttcag aaactcccgc accaccaaca tcggctcctg cgtgacgtga tggcccatcg
     9421 ggaactcgtg atactccggg atcacgccgt gctcctcgag gatgtggcgg gttcggcgcg
     9481 cgtatagaac tttgaccatg tcgtcctgcg tgccgtggat gacgagaacg gacaggtcgc
     9541 gatgtgcgcg aaagtcgggc atgtctgcct cgtagatcgc gccgctcatg acgacgatgc
     9601 ccgcgacttt ctgcttcgtg cggaaaccgg cgtcgatggc catcagaccg ccttgcgaga
     9661 atc
//
LOCUS       DATLVW010000687         5560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_278416, whole genome shotgun sequence.
ACCESSION   DATLVW010000687 DATLVW010000000
VERSION     DATLVW010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278416"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="mutY"
                     /locus_tag="VNN25_19955"
     CDS             complement(<1..1019)
                     /gene="mutY"
                     /locus_tag="VNN25_19955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXH93864.1"
                     /translation="MPTPRLASLTESWFRKHKRPLPWREGYEPYRVWVSEVMLQQTRM
                     EVVLGYYERFLRRFPTVGDLARASLDDVTAVWSGLGYYRRARMLREGAIDVMERFGGL
                     IPKSVDELMSIAGIGRYTAGAIASIAYGQRAPIVDGNVARIMARLFAFEAPLSAPALM
                     REAWVHAEELVASSKSPRDLNQGLMELGALVCTPRNPSCGICPLRRGCAAFSAGRVDE
                     FPRPKAKVATREMHIPLYLVVDREGRVLMRRESGQLMRGMFHLPHGDTTLLTGAPLRV
                     GVVETLGTFRHTVTNRRITFEVFAVTRRLRTRGREYAWIDPKRLAAIPHPSYVRKALQ
                     LAGIG"
     gene            1067..1867
                     /locus_tag="VNN25_19960"
     CDS             1067..1867
                     /locus_tag="VNN25_19960"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HXH93865.1"
                     /translation="MEDQAQTQQSETEQGKYVYCIIKTEIPRDFGPVGIGGRGDKVYS
                     VPYKEFAAVVSNCPLIVFDPTRENALAHEHVNELVMKDFTVLPMSFGTVFRTENDIKE
                     FLKGTYDALSDVLQKMDGKIEFGLKVNWDKDQVIREIETENEEIRRLKDEISSNTSTS
                     TYFSRMQLGRVVESALQAKADAYVSEIYEALRDAAIASRSNKPIGDKMIMNAAFLVDR
                     EKTKTFDDQITEIAKKYENKLSFLYTGPWPPYNFVNIRLKLERGQSGS"
     gene            1886..2197
                     /locus_tag="VNN25_19965"
     CDS             1886..2197
                     /locus_tag="VNN25_19965"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HXH93866.1"
                     /translation="MFLLDDILMLPIKLPIAGFNWVMKQIQTMANEELMNDQPWKERL
                     IELQMMLEVGDLTEEEYTQQEAQVFQALREIRARKEEIARQQRGDDDDSGIGIFTGYG
                     Q"
     gene            2194..4011
                     /locus_tag="VNN25_19970"
     CDS             2194..4011
                     /locus_tag="VNN25_19970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HXH93867.1"
                     /translation="MTLAAAFDQLAGRRVILIGGKGGVGKTTVSIAAALHLAASRKVI
                     LFTTDPASNLTDILHGELQTEALDAAALYRRFLDRNLDSFLELGDRGTYLDKDELRRF
                     FELSLPGVDELMAWMRIGELVAANPDATIVVDTAPTGHTLRMLSSADHFRQFAEALES
                     MQAKHRGMVRQFTRRDVRDAMDAFVDQFESEASRTRDLLPDSARTVFVPVTLSEPWVV
                     DQTERLTRDVRADGINVPFTVLNRAIADPDCERDRVRAERDAEARKRLAPIIDAPRSC
                     VPLDTAHAIAKWGSGVLRRSDSPVRHHEQVGLESPTYVKPARMMFLAGKGGVGKTTSA
                     VSIALQLAARSPDKHYTVISVDPAHTLRDVFAHQQPPPNLAVETIDTREKWRRFRDSL
                     GEEIDRAIGAITPGGMTVAYDAEAMKKLVEIAPPGADELFAITRLADLIADESQSGII
                     VDTAPTGHFLRLIDLPKTAGEWVREFMRILLRYRELIPAGSLAEELIRASRALHALEE
                     TMQSDATRVIVVTRPERIVIAESQRLIASVEERGMRIGGIIANYITPENDCACDQSLR
                     GFEQDALKQLGRDDVVEIARRDAPVTGLEELSGLVPLRS"
     gene            complement(4508..>5560)
                     /gene="eno"
                     /locus_tag="VNN25_19975"
     CDS             complement(4508..>5560)
                     /gene="eno"
                     /locus_tag="VNN25_19975"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXH93868.1"
                     /translation="AKEVEGMDAREQALIDLLMKQIDGTENKGNLGANAILGVSLAVA
                     HAAAQASGLPLFQYLGGPGARTLPVPMMNVINGGAHADNNLDVQEFMIIPAGFDDFSD
                     ALRAGAETFHHLKAILHDRKLTTAVGDEGGFAPDLKSNAEALDLILTAIEKAGYKAGE
                     QIFLGLDVAASEFFAKGKYGFEAESRSSDEMVAYYEQLRAKYPIISIEDGMSEGDWDG
                     WKTLTKAMGNGTQLVGDDVFVTNPSILAKGIEQGVCNAILIKVNQIGTLTETLDAIEM
                     AKRAGYRSVISHRSGETEDSTIADLAVATNAGQIKTGSLSRSERIAKYNQLLRIEEEL
                     GDAAVFAGKAIFRAKV"
BASE COUNT         1105 a         1789 c         1633 g         1033 t
ORIGIN      
        1 tggccgattc cggcgagctg caacgctttg cggacgtagg aagggtgcgg aattgcggcg
       61 agccgtttgg gatcgatcca cgcgtactcc cgacctcgcg tgcgaagccg ccgcgtcacc
      121 gcgaagactt cgaacgtgat cctgcggttc gtcaccgtgt gacggaatgt gcccagcgtt
      181 tcgacaacgc cgacgcgcag cggtgcgccg gtgaggagcg tcgtatcgcc gtgcggaaga
      241 tgaaacatcc cgcgcatcag ttggcccgat tcgcgccgca tcagcacgcg tccctcgcga
      301 tcgaccacga gatagagcgg aatgtgcatt tcgcgcgtgg cgaccttcgc tttgggccgc
      361 ggaaactcat cgacacggcc cgcagagaat gccgcgcatc cccgccgcaa cggacagatg
      421 ccgcaactcg gattgcgcgg cgtgcagacg agcgcgccca actccatcag cccctgattg
      481 agatcgcgcg gcgatttcga tgacgcgacc agctcctcgg catgtaccca cgcttcacgc
      541 atcagcgccg gcgccgaaag cggcgcttcg aacgcgaaga gccgcgccat gatccgggcg
      601 acgttgccat cgacgattgg tgcccgctgg ccgtaagcaa tcgaagcaat tgcgcctgcc
      661 gtgtaacgcc cgattcccgc gatcgacatc agctcatcga ccgacttcgg gatcaggccg
      721 ccgaagcgct ccatcacgtc gatcgctccc tcgcgcagca tgcgcgcgcg gcggtagtag
      781 ccgagtcctg accagactgc cgtgacgtca tccaggctcg cgcgagccag atcaccaacc
      841 gtgggaaagc gccgcaagaa ccgctcgtag tagccgagga cgacctccat cctcgtctgc
      901 tgcagcatta cctcggacac ccagacccgg tacggttcgt agccctcacg ccatggcagc
      961 gggcgcttgt gtttccggaa ccacgattcg gttagagagg cgagccgggg tgttggcaat
     1021 tcgcttgtat tgtatgcttt gcatgctctc catcgaggcc gccacaatgg aagatcaagc
     1081 acaaacacag cagtccgaga ccgagcaggg gaagtacgtt tactgcatca tcaagaccga
     1141 aatcccgcgc gacttcggcc ccgtcggaat cggtggccgc ggcgacaagg tctacagcgt
     1201 cccctacaaa gaattcgcgg ccgtcgtctc caactgcccg ttgattgtct tcgatccaac
     1261 gcgtgaaaac gccctcgccc acgagcacgt gaacgagctc gtgatgaagg atttcaccgt
     1321 gctgccgatg agtttcggca ccgtcttccg caccgagaac gacatcaagg agttccttaa
     1381 agggacttac gacgcgctct ccgacgtgct ccagaagatg gacgggaaga tcgagttcgg
     1441 cctgaaagtg aactgggaca aggaccaggt catccgcgag atcgagacgg aaaacgaaga
     1501 gatccgccgg ttgaaggacg agatctcatc gaacacgtcg acgtcgacct acttctcgcg
     1561 catgcagctc ggccgcgtcg tcgagtcggc actccaggcg aaagccgatg cgtacgtaag
     1621 cgaaatttac gaagcgctgc gcgatgccgc tatcgcttcg cgatcgaaca aaccgatcgg
     1681 cgacaagatg atcatgaacg ccgccttcct cgtcgaccgc gagaagacca aaacgttcga
     1741 cgaccagatc accgagatcg ccaaaaagta cgagaacaaa ctgtcgttcc tctacacagg
     1801 cccgtggccg ccatacaact tcgtgaacat ccggctgaag ttggagagag gtcagagcgg
     1861 cagttaagca acgggcaacc gtgccatgtt cctcctcgac gacatcctca tgcttcccat
     1921 caagctcccc atcgcgggct tcaactgggt catgaaacag atccagacga tggcgaacga
     1981 ggagttgatg aacgatcagc cctggaagga gcggctcatc gagctgcaga tgatgctcga
     2041 ggtcggcgac ctgaccgaag aggagtacac gcagcaggag gcacaggtct ttcaggcgct
     2101 gcgtgagatc cgggcacgaa aggaagagat cgcccgccag cagcgcggtg acgacgacga
     2161 tagcggcatc ggaatcttca ccggatacgg tcagtgacgc tcgccgccgc gttcgaccag
     2221 ctcgccggcc gccgcgtgat cctcatcgga ggcaaaggcg gcgtcggcaa gaccacggtc
     2281 tcgatcgcag cggcgctgca cctcgctgcg tcgcgcaaag tcattctctt cacgacggat
     2341 ccggcatcga atctgaccga catccttcat ggcgagctgc aaaccgaagc gctcgatgcg
     2401 gcggcgctct accgccgttt tctcgatcga aacctcgaca gttttctcga gctcggcgac
     2461 cgcggaacgt atctcgacaa agacgagctg cggcgtttct tcgagctatc gctaccgggc
     2521 gtcgacgagc tgatggcgtg gatgcgcatc ggcgagctcg tggcggcgaa ccccgacgcg
     2581 accatcgtcg tcgacaccgc gccgaccggc cacacgctgc gaatgctctc gtccgcggat
     2641 cacttccgcc agttcgccga agcactcgag tcgatgcagg ccaagcatcg cggcatggtc
     2701 cgccagttca caagacgcga cgtgcgcgat gccatggacg cattcgtcga tcagttcgaa
     2761 tcagaagcga gccgcacgcg cgacctgctc cccgactccg cgcgaaccgt cttcgtccct
     2821 gtgacgctgt ccgagccgtg ggtcgtcgat caaaccgagc gcctcacgcg cgacgtacgt
     2881 gccgacggaa tcaatgtccc attcacggtg ctcaatcgag cgattgccga tccggattgc
     2941 gaacgcgatc gcgtacgcgc ggagcgcgac gcggaggcgc gcaaacgatt ggcaccgatc
     3001 atcgatgcgc cgcgatcgtg cgtgccgctt gacaccgcgc atgcgatcgc gaagtggggc
     3061 agcggcgttt tacgtaggtc ggactctcca gtccgacatc acgagcaagt cggactagag
     3121 agtccgacct acgtaaaacc cgcgaggatg atgttcctcg ccggcaaagg cggcgtcggg
     3181 aagaccacct ccgccgtctc catcgctctc caactcgcgg cacgcagtcc cgacaagcac
     3241 tacaccgtca tctccgtcga tcccgcacac acgcttcgcg atgtcttcgc ccaccagcag
     3301 cctccaccca atctcgcagt cgagacgatc gacacgcgcg agaagtggcg ccggttccgc
     3361 gattccctcg gcgaggagat cgaccgcgcc atcggggcca tcacccccgg cggaatgacc
     3421 gtcgcctacg acgccgaggc gatgaagaaa ctcgtcgaga ttgcgccgcc gggcgccgac
     3481 gagctcttcg caatcacccg gctcgccgat ctgatcgccg acgagtcgca gtccggcatc
     3541 atcgtcgaca ctgcgcccac cggccatttc cttcgcctca tcgatctccc aaagaccgcc
     3601 ggcgagtggg tgcgcgagtt catgcgcatc cttcttcgct accgcgagct gatccctgcc
     3661 ggctcgctgg ccgaggagct gattcgcgcg tcgcgtgcgc ttcacgcgct cgaggagacg
     3721 atgcaatcgg acgccacgcg cgtcatcgtc gtcacgcggc cggagcgcat cgtcatcgcc
     3781 gagagccagc gcctcatcgc atcggtcgaa gagcggggta tgcggatcgg cggcatcatc
     3841 gccaactaca tcacgccgga gaatgactgc gcgtgcgatc aatcgcttcg cgggttcgag
     3901 caggatgcgt taaagcagct cggccgcgac gatgtcgtcg agatcgcgcg ccgcgatgcg
     3961 ccggttacgg ggctcgagga actgagcggg ctcgttcctc tgcgcagtta gaacgaaacc
     4021 atcacgacag cgtcgtcgtg accaatagga cgatgccctc aaaacaggac acccgcccct
     4081 ttatcaagct gttccttcgc tttttcgcgt gagatagcga taactgacat ccattcagta
     4141 gtttgcgtcg ctcaatggtc gaactaatta gttccccgct cgaactaatt agttcccggg
     4201 tcgaactaat tagttccccg accgaactat ttagctcccc ggcccgagct atttagttcc
     4261 cagcgcgaac tatttagttc ccgggccgaa ctatttggtt cccgggccga actatttagt
     4321 tcccagtctg aactatttag ttccccgacc gaactaatta gttcccggat cgaactattt
     4381 agttcctcgg cctgaactat ttagttcccc agccgaacta tcgagttcgg gggccaaact
     4441 atttagctcc gaggcgaacc tcgacatctg cgagaccgat gtgagatctc agtggcgcga
     4501 tgtcttttta caccttcgcc ctgaaaatcg ccttccccgc gaacaccgcg gcatcgccca
     4561 gctcctcttc gatgcggagc agttggttgt acttggcgat gcgctcgctg cgcgagagtg
     4621 atcccgtctt gatctgcccc gcgttcgtgg cgacggcaag gtcggcgatc gtcgaatcct
     4681 ctgtctcgcc ggagcggtgg gaaatgaccg agcgatagcc ggcgcgcttg gccatctcga
     4741 tcgcgtcgag cgtttccgtc agcgtgccga tttgattgac cttgatgagg atggcgttgc
     4801 agacgccctg ctcgattcct ttcgccagga tcgacggatt ggtgacgaag acatcgtcgc
     4861 cgacgagctg tgtgccgttg cccatcgctt tcgtcagcgt cttccagccg tcccaatcgc
     4921 cctcggacat cccatcctcg atcgagatga tcgggtactt cgcgcgaagc tgctcgtagt
     4981 aggcgaccat ctcgtcgctg ctgcgcgatt ccgcttcgaa gccgtacttc cctttggcga
     5041 agaactccga ggcggcgacg tcgagaccga ggaagatctg ctcgccggcc ttgtatcccg
     5101 ccttctcgat tgcagtcagg atcagatcga gcgcctcggc gttcgattta agatcgggcg
     5161 cgaacccgcc ttcgtcgccg accgctgtcg tcagcttgcg gtcgtgaagg atggctttga
     5221 ggtgatggaa ggtctcggcg ccggcacgaa gagcgtcgct gaaatcgtcg aagccggccg
     5281 ggatgatcat gaactcctgg acgtcgaggt tgttatcggc gtgggcaccg ccgttgatga
     5341 cattcatcat cggcacgggc agcgtgcgcg cgccgggtcc gccgaggtat tggaaaagcg
     5401 gcaggccgga agcctgcgcc gcagcgtgcg ctacggccag cgagacgcca agaatcgcgt
     5461 tcgctccgag gttgcctttg ttctccgtgc cgtcgatctg cttcatcagg agatcgatga
     5521 gcgcctgctc gcgggcgtcc atcccctcga cttccttggc
//
LOCUS       DATLVW010000688         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_439975, whole genome shotgun sequence.
ACCESSION   DATLVW010000688 DATLVW010000000
VERSION     DATLVW010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439975"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..356)
                     /locus_tag="VNN25_19980"
     tRNA            complement(280..356)
                     /locus_tag="VNN25_19980"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Met,seq:cat)
     gene            426..632
                     /locus_tag="VNN25_19985"
     CDS             426..632
                     /locus_tag="VNN25_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93869.1"
                     /translation="MSDVDERRCIRCSDPEESTRLERCVVCGKHFCHDCAYRATGRRF
                     CSPECARAFFYGESDDDEDTDAAG"
     gene            601..1626
                     /gene="hemB"
                     /locus_tag="VNN25_19990"
     CDS             601..1626
                     /gene="hemB"
                     /locus_tag="VNN25_19990"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXH93870.1"
                     /translation="MTTKTLTLPVETTTTQTTRRPRRLRRTDSIRAMVRETVVRAEDL
                     IYPLFVVPNSRPKIEIGSMPGVWQLRAHEAVDQALRAHDLGIRALLLFGLPEYKDGIG
                     SASWDPAGPVQSAIEAIKRAVPQMTVMADVCLCEYTDHGHCGVIIDHNGVKDVDNDQT
                     LPLLAKQAVSFAAAGADFVAPSDMMDRRVGAIRTALDDASLTDVGIMAYSAKYASGFY
                     GPFREAAESAPQFGDRRTYQMDPANVREAIREMELDVEEGADMIMVKPALSYLDVIRV
                     ARDNFDLPVAAYNVSGEYSMVKAAAERGWIDGPRVMNEILTSIKRAGADLIITYHAME
                     WAELQGR"
     gene            complement(1696..1947)
                     /locus_tag="VNN25_19995"
     CDS             complement(1696..1947)
                     /locus_tag="VNN25_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93871.1"
                     /translation="MRIRRALSAALVVASMTIAIPLQAGYCMKQDQACCKDHKSCKKE
                     HMDCCKGSAKLHSCTYKKDCCGDSCGMPKKAAEPAQKPS"
     gene            complement(2062..>2270)
                     /locus_tag="VNN25_20000"
     CDS             complement(2062..>2270)
                     /locus_tag="VNN25_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXH93872.1"
                     /translation="TGCGGSGYRLAYADGPIDGDQIIDIDGIPVALDDMSASRLDGAI
                     IGFNADDDGYLLDHPDAVAAVWCG"
BASE COUNT          426 a          657 c          757 g          430 t
ORIGIN      
        1 cgcgagtccg acctttgaat tcccacggcg tctggtccac acgcccacaa ggtagggccg
       61 aaatcggtga gaatccagtg tgcgtgccgc gcgaatcaaa gcatcaacct tggctaatcg
      121 cgggtatcga acagtcactt aaagtgatga ttccaataaa cttgctggcg tgcgaaccga
      181 gagtcacgca tcagaaattc atgcttgcgc acgcttgccg gtgtcacgtg acacgcacga
      241 ctatgatgcg tctggttgga catggtaaga gacgttgttt ggtggcggtg cccagagtcg
      301 aactggggac ctagcgctta tgagacgccc gctctaacca tctgagctac accgccatcg
      361 ggagggctgc cggaggatat actcaacccg gcgcgcggtg aaaaatttcg cgcgggagcg
      421 gctgaatgtc cgacgtcgac gagcggcgat gcatccgctg cagcgatccc gaggagagta
      481 cgcggctgga acgctgcgtg gtctgcggga agcacttctg ccacgactgc gcttaccgcg
      541 ccacgggacg gcgcttctgc tcccccgagt gtgcgcgcgc ctttttctac ggagagagtg
      601 atgacgacga agacactgac gctgccggtt gagaccacga ctacccagac gacgaggcga
      661 ccgcgccgcc tgcgccgcac cgattcgatc cgcgcgatgg ttcgcgagac ggtcgtgcgc
      721 gccgaggatc tgatctatcc gctgtttgtc gtgccgaaca gccggccgaa gatcgagatc
      781 ggatcgatgc cgggcgtgtg gcagcttcgc gcgcacgagg ccgttgacca agccctccgc
      841 gcccacgacc tcggcatccg tgcgctgttg ttgttcgggc tgcccgagta caaagacggg
      901 atcggctcgg catcgtggga tccggccggc ccggtgcagt cggcgatcga ggcgatcaag
      961 cgcgccgttc cgcagatgac ggtgatggcc gacgtctgcc tctgcgagta caccgaccat
     1021 ggacactgcg gcgtcatcat cgaccacaac ggggtgaagg atgtcgacaa cgatcagacg
     1081 ttgccgctgc tcgcgaagca agcggtttcc ttcgccgcgg ccggcgccga cttcgttgcc
     1141 ccctcggaca tgatggaccg gcgcgtcggt gcgatccgca cggcactcga tgatgccagc
     1201 ctcaccgacg tcgggatcat ggcctactcg gcgaaatacg cgtcggggtt ctacggtccg
     1261 ttccgcgagg ctgccgagtc agcgccgcag ttcggcgacc gacgcacgta tcagatggat
     1321 ccggcgaacg tgcgcgaagc gatccgcgag atggagctgg acgtcgaaga gggtgccgac
     1381 atgatcatgg tcaagccggc gctttcgtat ctcgacgtga tccgcgtggc gcgcgataac
     1441 tttgatctgc cggtggcggc ttacaacgtg agcggcgagt attcgatggt gaaggcggcg
     1501 gcggagcggg gatggatcga tgggccgcgg gtgatgaacg agatcctgac gtcgatcaaa
     1561 cgcgcgggtg cggatctgat catcacgtac cacgcgatgg agtgggcgga acttcaggga
     1621 cggtaagagt cggtcgccgc cgggccggcg gcggaacagc cgtcgggccg acggcgctac
     1681 gaaagaaaga cggcgctacg agggtttctg cgcaggttct gcggctttct tgggcattcc
     1741 gcaggagtcg ccgcagcaat cctttttgta cgtgcaggag tgaagcttcg ccgagccctt
     1801 gcagcaatcc atgtgctctt tcttgcagct cttgtggtcc ttgcagcagg cttgatcctg
     1861 cttcatgcag tagccagcct ggagagggat ggcgatggtc atcgatgcta cgaccagcgc
     1921 tgcggagagt gctctgcgga tcctcatgac tcctccgagt gaatggacaa ttgataatgg
     1981 acaattgaca acgaaagacg ggcttggtgg tgttcggacc gtctcaattg tcaattttca
     2041 attgcccatt gtcaattgcc ctcacccgca ccatacggca gccactgcgt cgggatggtc
     2101 gaggaggtag ccgtcgtcgt cggcgttgaa tccgatgatg gcgccgtcga ggcgggaggc
     2161 ggacatgtcg tcgagggcga cggggatgcc gtcgatatcg atgatctggt cgccgtcgat
     2221 cgggccgtcg gcgtaggcga gtcgatagcc gctcccgccg cagccggtgg
//
LOCUS       DATLVW010000689         6675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_482416, whole genome shotgun sequence.
ACCESSION   DATLVW010000689 DATLVW010000000
VERSION     DATLVW010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482416"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..665)
                     /locus_tag="VNN25_20005"
     CDS             complement(39..665)
                     /locus_tag="VNN25_20005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH93873.1"
                     /translation="MTTPLPPDVECEKQHPVLAVSDVVAAAQFYTAKLGFKLAFIAGD
                     PPEIAGLNLGHVQMFLEPGKPAPDGCAVYFVVGDADELFEFQRANGVDVVTNPGDREY
                     GLRDYTVRDLYGYELRFGHYIYNVGPAIEIERVDVSVRLEKRLAALLHDLAEHNHVSL
                     TSLFEETFLHTLDGVGPHGDRTLKYIQKLKEKHAIDYDSHGSYRFVER"
     gene            complement(682..1086)
                     /locus_tag="VNN25_20010"
     CDS             complement(682..1086)
                     /locus_tag="VNN25_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93874.1"
                     /translation="MIKKVTPVLIVDRIEPLLPLWDALGFARAAEVPHGDVLGFVILT
                     RDGVEVMYQTFASVRGDEANVLDGPRAIGAAAVFIEVDDIEALAALIPSGTDVVVARR
                     KTFYGSTEIVIRDGAGNVITFAQFAQESTGAD"
     gene            1130..2749
                     /locus_tag="VNN25_20015"
     CDS             1130..2749
                     /locus_tag="VNN25_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXH93875.1"
                     /translation="MKKALCLAVLALSVVPLFAQSPEAAPSRPTAPPSARRDIAAAPD
                     SEDRGDRPETTRAGDDAKSGRPPADAKDKISTTQHTVTIGGQTISYTARAGTMIMKDE
                     EGKPRASFFFTSYTKDGADPARRPVTYTFNGGPGSSSVWLHMGAFGPKRVAYADDMGN
                     AARPPYRTVDNEGSILDVTDLVFIDPVTTGYSRAIPFADASKFHGVDSDIQSVGEFIR
                     LWTTRYGRWSSPKFLAGESYGTTRAAGLSGWLASQGFYLNGIVLISSILNFETASFDS
                     GNDLAYELFLPTYTAIAWYHKRLPTDLQSGTLEHAVAESEQFALGEYTHALMQSDRIS
                     DQERHEVAAKVARLTGLSPEYIDRANLRVRIDRFDKELLRNQRRIVGRLDGRFIGIDR
                     DAAGESTEQDPSYSAIFGEYTAVLNDYVRRELKFDTDLPYEILTGKVRPWSYDRASNR
                     YVDVAETLRGAIAQNPYMKVFVANGYYDLATPFAATRYTFARMQLDPDLRKNISMDYF
                     ETGHMVYIDRKAQARLKKDVDEFIRANANVQ"
     gene            2746..2994
                     /locus_tag="VNN25_20020"
     CDS             2746..2994
                     /locus_tag="VNN25_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93876.1"
                     /translation="MTVFREAFEVVGRIEAIETIASGSGIRDLQQLIDAYGPGRWRKL
                     KGVATVRVESGRIRLAELHWYEAHGIGKRDMKVKRYFD"
     gene            2996..3244
                     /locus_tag="VNN25_20025"
     CDS             2996..3244
                     /locus_tag="VNN25_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93877.1"
                     /translation="MSDTSRFAICIRNTDYAVSLKPLKVYPVLDDLDAEQDGMIRIID
                     ESGEDYLHTAARFVVVTIPSMDARALLQAIETLTPTHH"
     gene            3267..4028
                     /gene="surE"
                     /locus_tag="VNN25_20030"
     CDS             3267..4028
                     /gene="surE"
                     /locus_tag="VNN25_20030"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXH93878.1"
                     /translation="MARILVTNDDGIYSEGLRTLADACRRIDGVEVLVVAPDREQSAA
                     SHALTLNRPLRLLKLRESEYIIDGTPADCVNLAILKLMKDDRPDLVVSGINFGPNLGD
                     DVTYSGTISAAFEGALLGIPSIAFSTLVGEGFSFERCAEVAEKLIRWALVSHRDPRII
                     LNVNFPVAFTGVRVARLGTRIYSEGIIERKDPRGRTYYWIGGGEPTWHPAEGTDFEAV
                     ESGCIAITPLHLDLTHHESIPRLKSLEELLGNTNE"
     gene            4069..4683
                     /locus_tag="VNN25_20035"
     CDS             4069..4683
                     /locus_tag="VNN25_20035"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXH93879.1"
                     /translation="MVQRYVTDRGIKDERILRAMREVPRHLFVPTLVASQAYGEKALP
                     IGAKQTISQPYIVARMIELLDLTGKEKVLEIGTGTGYQAVVLSKLCAKVFSIERINEL
                     ALRAVELIRTLKIHNASVKVFDGTYGWSDQAPFDRIIVAAAAPEVPEPLLQQLSRTGK
                     LVIPIGPEGKQRLARVMRVGTSLKVEDCGSAEFVPLVGKFGWKE"
     gene            4759..4953
                     /locus_tag="VNN25_20040"
     CDS             4759..4953
                     /locus_tag="VNN25_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93880.1"
                     /translation="MQCEKCGSDVKCHHGGGKSGCGSLWGIGMGIAGTLSWLRWHSVM
                     WVILHGIASWFYLLYRWFFA"
     gene            6202..6645
                     /locus_tag="VNN25_20045"
     CDS             6202..6645
                     /locus_tag="VNN25_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93881.1"
                     /translation="MRRFLLITTAIVSALLGAVVAWLVLTIPNDLQAGALLRTARNDL
                     EAGRNDRARQEYSRVIQQYPRTDAAAAATVALASIADSERHILAAALDQQRRILAAQQ
                     KQITDLAAQINAIRTAPPPAPVVVQAPPPPPKKTPAKHTPRKRHR"
BASE COUNT         1325 a         2146 c         1946 g         1258 t
ORIGIN      
        1 ctcattcgaa tccggatgag ccgctctctt cattccacct accgttccac gaaccgataa
       61 ctcccgtgac tgtcgtagtc gatggcgtgc ttttccttca gcttctgaat gtacttgagc
      121 gtgcggtctc cgtgcgggcc gactccgtcg agcgtgtgca ggaacgtctc ctcgaacaga
      181 ctcgtcagac tcacgtggtt gtgctcagcc aggtcgtgaa gcagcgcggc cagccgcttc
      241 tccaggcgca cggaaacatc gacgcgctcg atctcgatcg cgggacccac gttgtagatg
      301 taatgtccga agcgaagctc gtagccgtaa agatcgcgaa ccgtgtagtc gcgaagcccg
      361 tattcgcgat cccccggatt cgtgacgacg tcgactccgt tggcgcgctg aaactcgaac
      421 agctcgtccg catcgccgac gacgaaataa accgcgcagc catccggggc aggcttcccc
      481 ggctccagaa acatctgaac gtggccgagg ttcaggcccg caatttcggg cggatcgccg
      541 gcgatgaagg cgagcttgaa gccgagcttc gccgtgtaga actgtgcggc cgcaacaacg
      601 tcgctgaccg ccaggaccgg gtgttgcttt tcgcactcga catcgggagg cagtggagta
      661 gtcatgagag tccctcgtgc gtcaatccgc gccagtcgat tcctgcgcaa actgcgcgaa
      721 cgtgatcacg ttgcccgcgc cgtctcgaat cacgatttcc gttgagccgt agaacgtctt
      781 tcttcgcgcg acgaccacgt cggtgccgga tgggatcagc gcagccagcg cctcgatgtc
      841 gtcaacctcg atgaacaccg ctgcggcgcc gatcgcgcgc ggtccatcga gtacgttcgc
      901 ttcatcgccg cgcacgctgg cgaaggtctg gtacatcacc tcgacgccgt cgcgcgtcag
      961 gatcacgaac ccgaggacgt cgccatgcgg cacctcggcg gcgcgagcaa agcctagggc
     1021 gtcccatagc gggaggaggg gctcgatgcg gtcgacgatc agtacgggcg tcactttctt
     1081 gatcatcgga taacgatagc gtcacgggcg ctgccataca attcgcccca tgaaaaaagc
     1141 gctttgcctc gcggtcctcg ccctgtctgt tgttcccctc ttcgctcagt cgccggaagc
     1201 cgcaccgagc cgcccgacgg caccaccaag cgctcggcgg gacatcgccg ccgcgccgga
     1261 tagcgaagac cgaggcgacc ggccggagac gacaagggcg ggagacgacg cgaagagcgg
     1321 cagacctcct gccgacgcga aagacaagat ctcgacgacg cagcacaccg tcaccatcgg
     1381 cggccagacc atcagctaca cggcgcgcgc cggcacgatg atcatgaaag acgaggaagg
     1441 aaagccgcgc gccagcttct tcttcacctc ctacacgaaa gacggtgccg atcccgcgcg
     1501 ccggccggtc acctacacct tcaacggcgg ccccggatcg tcgtcggtgt ggctgcatat
     1561 gggcgcgttt gggcccaaac gagttgctta cgcggatgac atgggcaacg ccgcccggcc
     1621 gccctatcgc accgtcgaca acgagggaag cattctcgac gttaccgatc tcgtcttcat
     1681 cgatccggtc acgaccggct acagccgggc cattccgttt gccgacgcga gcaagtttca
     1741 tggcgtcgat tccgatatcc agtccgtcgg cgaattcatc cgcctctgga cgacgcgcta
     1801 cggccgctgg tcatcgccga agtttctcgc cggcgaaagc tacggcacca cgcgcgccgc
     1861 gggtctttcg ggctggctcg catcgcaggg cttctacctc aacggcatcg tcctcatctc
     1921 ctcgatcctc aacttcgaga ctgcctcgtt cgacagcggc aacgaccttg cctacgagct
     1981 ctttctcccg acgtacaccg ccatcgcctg gtaccacaag cgtcttccca ccgatctgca
     2041 aagcggcacg ctggagcacg ccgtcgccga gtccgaacag ttcgccctcg gcgaatacac
     2101 ccacgcactc atgcaaagcg accggatcag cgatcaggag cgccacgaag tcgccgcgaa
     2161 agtggcccgg ctgaccggtc tctcgccgga gtacatcgac cgcgcaaatc ttcgcgttcg
     2221 tatcgatcgt ttcgacaagg agctcctgcg caaccaacgc cgcatcgtcg gccgcctcga
     2281 tggccgcttc atcggcatcg accgagatgc cgcaggcgaa tcgaccgagc aggatccgtc
     2341 gtactccgcc atcttcggcg agtacaccgc cgtcttgaac gactacgtcc gccgcgagct
     2401 gaaattcgac accgacctgc cgtacgagat cctcacaggc aaagtgcgtc cctggagcta
     2461 cgaccgagca tcgaatcgct atgtggacgt cgccgaaacg ctgcgcggcg ccatcgccca
     2521 gaacccgtac atgaaggtct tcgtcgccaa cggctactac gacctggcca cgcctttcgc
     2581 cgccacccgc tacaccttcg cacgcatgca actcgacccc gacctgcgca agaacatctc
     2641 gatggactac ttcgagaccg gccacatggt ctacatcgac cgcaaagcgc aggcgagatt
     2701 gaagaaggac gtggacgagt tcattcgggc gaacgcgaac gtgcaatgac cgtgtttcgc
     2761 gaggcgtttg aagttgtcgg acggatcgaa gcgatcgaga caatcgcctc tggaagcggg
     2821 attcgtgatc tacagcagct catcgatgct tacggccccg gccgctggcg taaactaaag
     2881 ggcgtcgcca cagtgcgcgt ggagtcaggg cgcatccgtc ttgcagaact acactggtac
     2941 gaagcgcacg gcattggaaa acgcgatatg aaggttaaac gctacttcga ctgaaatgtc
     3001 agacacatct cgctttgcca tctgcatcag aaacaccgac tacgcggttt cgctcaagcc
     3061 gctgaaggtc taccccgtac tcgatgatct cgatgcggag caggacggaa tgattcgcat
     3121 catcgacgag tcgggcgagg attacctgca taccgccgcg agatttgtcg tagtaaccat
     3181 tccgtccatg gatgctcggg cgttgcttca ggcaatcgaa acgctcactc cgacccacca
     3241 ctgacctgcg ctaccatgcg cgcgccatgg cccgcatcct ggtcaccaac gacgacggca
     3301 tttacagcga aggactgcgc acgcttgcgg atgcttgccg gcgcatcgat ggcgtcgagg
     3361 tgctggtcgt cgcgcccgat cgggagcaga gcgcggcgtc gcatgcgctc acgctgaacc
     3421 ggccgctgcg attgctcaaa ctgcgcgaga gcgaatacat catcgatggc acccccgccg
     3481 actgcgtgaa cctggccatc ctcaagctga tgaaggacga ccgccccgat ctcgtcgtct
     3541 ccggcatcaa cttcggaccc aacctcggcg acgacgtcac ctactcgggg acgatctctg
     3601 ccgcctttga gggtgcgctg ctcggcatcc cttcgatcgc attctccacg ctcgtcggcg
     3661 aaggattctc gttcgagcgc tgcgcagagg ttgcggagaa actcatccga tgggcgctcg
     3721 tttcacaccg cgatccgcgc atcattctca acgtcaactt ccccgtcgcc ttcaccggcg
     3781 tgcgcgtggc ccggctcgga acgcgcattt actccgaggg aatcatcgag cggaaagacc
     3841 cacgcgggcg gacgtactac tggatcggcg gcggcgagcc gacctggcat cccgccgagg
     3901 ggacggactt cgaggcggtg gagagtggat gcatcgccat caccccgctg cacctcgacc
     3961 tcacccatca cgaatccatc ccgcggctca aatcgctcga ggagctcctt ggcaacacga
     4021 acgaatgacg ccgcgccgaa gcccgacttc gcccaccttc gcgagggcat ggtccaacgc
     4081 tacgtcacgg acagaggcat caaagacgag cgaatcctgc gggccatgcg cgaggtgccg
     4141 cggcatcttt tcgttcccac gctcgtcgcc tcgcaggcct atggcgagaa ggcgctcccg
     4201 atcggcgcga aacaaaccat ctcgcagccg tacatcgtcg cgcggatgat cgagctcctc
     4261 gacctgaccg gaaaagagaa agtgctcgag atcggcaccg gcaccggcta tcaggccgtc
     4321 gtgctctcga agctctgcgc gaaagtcttc tcgatcgagc gcatcaacga gctggcgttg
     4381 cgcgcggtcg agttgatccg caccctcaag atccacaacg cctcggtgaa agtattcgac
     4441 ggcacctacg gctggtccga tcaggcgccc ttcgatcgca tcatcgtcgc cgccgcggcg
     4501 ccggaagtgc ccgagccgct gttgcagcaa ctctcgcgca cgggcaagct ggtcatcccg
     4561 atcggtcccg agggaaagca gcggctcgca agagtgatgc gggtcgggac gagcctcaag
     4621 gtggaagact gcggatccgc cgagttcgtg ccgctagttg ggaagttcgg ctggaaagag
     4681 tagcggcgga aaacgctcga ggatcggtta gcggtccagt ttgatggata ggttgtgaca
     4741 atcttctaga atgcttccat gcagtgcgag aagtgtggca gcgatgtgaa gtgccatcac
     4801 ggtggtggca agagcggctg cgggtctttg tggggaattg gcatggggat cgccggaacg
     4861 ctctcctggc tccggtggca ctcggtgatg tgggtgatcc ttcacggcat cgcaagttgg
     4921 ttctacctgc tctaccggtg gtttttcgcg tgaacggcga ttgccctatg cggttatgtg
     4981 gcaacccctt cgctccacat gtagcggaag ccggattgtc acgacgcaat cacgcttctt
     5041 cgagataggc actttgacgt cccggcaacc gggcgaccgc atggccatcc tcatcccggg
     5101 agtgagagtg agatcttttc gagtccgcac tcgaatccgg tgagctggta ctcaaacttc
     5161 tcgagtcggt acctacatcg tttgagtccg gactcacatc cgatgagccc ggactcacgt
     5221 ccggtgagtc ggtactcacg taggatgagt cggtactcaa gtgacgcgag tcggtactca
     5281 accgacgtga atccggactc acaacctttg agtcggtact catatctttt gagtccgtat
     5341 tcaagttcga tgaatccgta ttcaagttcg tcgagtccgt actcaagttc gttgagtccg
     5401 tactcaagtt ggttgagtcc gtattcaagt tcggtgagtc ggtactcatg tcttttgagt
     5461 ccgtactcat actctttgag tcggtgctca catcttttga gtccgtactc aagttcgatg
     5521 agtccgtatt caagttcgtt gggtcggtac tcatgttttt tgagtacgga ctcatatttc
     5581 ttgagtcggt actcaaattt gaaatcgacc gagtggcctt cggatggtcc gaatcacctg
     5641 attttggtag aaaacgggcc gaaatcgacg gagtgctgcc ggaactgtga tcagttattc
     5701 ccggctcggg ctatttgatc gcccctcgat gcgccgggca gcgcgacgga agattcgccg
     5761 tcacaacccg acggatgcct ccacgcccgc gctgggaatg agccagccaa attggactgg
     5821 cgcatagctg gcctgcattc ctcacacttc ttcagggact cttttgcgtc gagcgccttc
     5881 tcccctcgtg cttttcgagg ggagaaggtg ccgaaggcgg atgaggggtg tcgtgcaatc
     5941 ggacggcacg aagtttccgg tcggcggaag tcactgcaca gtgcccttcg gcaacgctgt
     6001 ggagaagtca aatcccccct tgtccggctt cgccaccttc tccccccgca aaaagcgcgg
     6061 ggggagaagg cgctcgattg gaaggaatcg aagaaatgtg tgacaagtgc gagctagcgg
     6121 cggcgttcgt gatcgtggac gaagacggtc caaggatcgc aactgagctg gcgccgctct
     6181 tgaagcaccc ggctcatgcg tatgcgccga ttccttctga tcaccacggc gatcgtctcc
     6241 gcactcctcg gcgctgtcgt cgcctggctc gtgctcacga tcccgaatga cttgcaggcc
     6301 ggcgcgctgc tccggacggc gcgaaatgac ctcgaagcgg ggcgcaacga cagagcacgc
     6361 caggagtact cgcgcgtcat tcagcagtat ccgcgcaccg acgccgcggc cgcagcaacc
     6421 gtcgcgcttg cctcgatcgc cgacagcgag cggcacatcc tcgccgctgc gctcgaccag
     6481 cagcggcgca tcctggccgc gcagcagaag cagatcaccg acctggccgc gcagatcaac
     6541 gccatccgaa ccgcgccgcc gccggcacca gtcgtcgttc aggcaccacc gcccccgccg
     6601 aagaaaacac ccgcgaagca cacgccccgc aaacggcacc ggtaattgcg tacaatctcc
     6661 gcgcacatgg ccagg
//
LOCUS       DATLVW010000690         9765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_73560, whole genome shotgun sequence.
ACCESSION   DATLVW010000690 DATLVW010000000
VERSION     DATLVW010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73560"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2512)
                     /locus_tag="VNN25_20050"
     CDS             complement(<1..2512)
                     /locus_tag="VNN25_20050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXH93882.1"
                     /translation="MPTFNAHLPRRRTVFQAISITVVVILVSILGLTLYVYKQSVGKF
                     EIRRLSLPTRIFTDFTPLRGGVVLAPDDLVEKLIRLGYRQADSLTRSGDFVPGSNQID
                     VYTRPFTHPTGEYPSQPVRIAFKNGAISSVVSLRQASPIDNAALEPELLTSILSDQLE
                     NRRPVTLDQVPKSLQDAVVATEDVRFWHHPGVDPIGLFRALFRNIRAGGVAEGGSTLT
                     QQLVKNYYLTNERTYKRKATEAFMAVILDAKYSKKEIIEAYLNDIYLGRNRSISILGV
                     GEAARFYFGKPVSELTLAESALLAGMIRSPNNYSPFTRPDLAMQRRSTVLGLMLNQKK
                     ITRDEYDKAMATKLPPRPFRQRSGLSSIPYYVDRVLQEMSSDYGIKNVKGRGLQIYTA
                     IDLNAQDDAARTIEAGLSALEKGSKRLRRPDQPLQGALINVDVPTGEIRALIGGRNYD
                     INQFNRALNAKRQIGSLVKPFVYATAFEPSLSSQNITPATLVSDTRFVYKRQFSEDWS
                     PRNYEDVYHQTVTVAQALEQSLNSASVRIGLACGLDPIVKTAHTLGLTSDIDSKNPSM
                     LLGAIDVAPIEMADAYATIARVGSRVPLHTVKFVMDDRNHVLSAGGDVQAVQVFPARD
                     MYILLTVMKGVVDRGTAAATRSMGFKLIAAGKTGTTNDKRDAWFIGFTPKTLTLTWIG
                     FDDNQPTGLSGGSGAVPIWTRYMQSVTAGQPNADFAPPDGIAMAEIDETSGGLAVPAC
                     PPSSVVSEAFKSGTQPMNPCPLHSPQAAPAPAVDQFGNPIALDTAGMPVSPEVTTTGF
                     APPLPPVPPASGDNTLGGGIFKTDTTATTTSGEPRRDQV"
     gene            complement(2651..3388)
                     /locus_tag="VNN25_20055"
     CDS             complement(2651..3388)
                     /locus_tag="VNN25_20055"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HXH93883.1"
                     /translation="MNWIIEQSEIGRIVVPPSLPERLGLFYTTVDFEGRLNESVANQL
                     MRLVHQRFGIEATLSSCTQIHGKKAVNATAGERWQECDSCDALWSASKGAAIAIKIAD
                     CLPVSIADSKNRVIANIHSGWRGAAQRIVAETLDVIERETEFDAATAQAWLGPTIRAC
                     CFEVGEEVVDQLRAAYSDAESFVDRSCAKPHVDVAGLTAALLIQRGFAPENVHDSGLC
                     TRCDDSIFHSYRRDPKRGGRNLALIAR"
     gene            3431..3625
                     /locus_tag="VNN25_20060"
     CDS             3431..3625
                     /locus_tag="VNN25_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH93884.1"
                     /translation="MPTKVREAVRMIEQDGWVQVKSRGGHRQFKHPKKPGRVTISGGS
                     SDDIAPGTMNSILKQAGLKR"
     gene            3628..3852
                     /locus_tag="VNN25_20065"
     CDS             3628..3852
                     /locus_tag="VNN25_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXH93885.1"
                     /translation="MHRFLIIIEDAGANFSAYSPDLPGCAATGKTRSDAEANMYEAIQ
                     MHIEGLREDGLPVPESTAFAEYMLIGAESA"
     gene            complement(3859..4761)
                     /locus_tag="VNN25_20070"
     CDS             complement(3859..4761)
                     /locus_tag="VNN25_20070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH93886.1"
                     /translation="MGRRTTPDRAEPACRLVRFEAADDVALAGVLYEPSRATKRAVIW
                     LHGLGGSVFESRRTNFLAQTFIDRGIAFFPFNNRGSGLVRRAGPTLGGASFERIRDCV
                     ADVDGAIRELWRRGYRDLTLAGHSTGANKIAVYDYYKPRNRVKRYVLIAGGDDTGLLY
                     DQLGKRRFEAMLIKSRSMIKARRGDELAPRNAGSPMMSWRSLYDMANPNGDYNVFPFL
                     EAMRGVKLSRRPLFRHIRGIRKTSLYIYGDRDEYCFGDVPRCVAILSEHVNERAELIV
                     MRDADHGFSGREGELGTLIAEWIG"
     gene            complement(4710..5657)
                     /locus_tag="VNN25_20075"
     CDS             complement(4710..5657)
                     /locus_tag="VNN25_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93887.1"
                     /translation="MMHFHNGDVSASLARRAGVPGRHVPFRESLVGGPVQPTLPLHEW
                     VESRAAFLSEQYGEHLLRVRNELLEQENVIDRAHEEEEVVLWFEHDLFCLVHLLYLFS
                     RLSKVRRISLIWCAEPLGTQSEEDVFARFDSRAAVTPAMNTAAKLAWSAFTNADPTSL
                     NRFLSADVADFPFLRDGFLLHASRFPSTRNGLGEVERRAVEGIDAGASDFVSLFTRFD
                     QAPPRFGFGDGEFLRHLKHLAGAAVPVITIIGEEKANPPKALFTLTPAGRNMIEGKID
                     FVEINNPSFWLGGTYLTRERMWRWDGERRQIVPSPPAAS"
     gene            complement(5862..8540)
                     /locus_tag="VNN25_20080"
     CDS             complement(5862..8540)
                     /locus_tag="VNN25_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93888.1"
                     /translation="MIRGFAFIALLLATAAVAQAPHGAWRTITTPHFRVHYPAQYEAW
                     SIRAASRLESVRTAVVAEVGFAPETVTDVIVENPIAEANGATIPLLDTPRIVLYPEAP
                     GPEEVIGEYSNWIDLLTVHETAHLVHLLRPSRNPFERAMAHLLPLNPITLHAPRWVLE
                     GYATVIEGRITGSGRPSGAMRAAVLRMWAMNGELPKYDQLDSDPRFLGGSMAYLAGSA
                     FLEWLAEKSGPDSLRHLWARMTARRRRSFDEAFTGVFGDSPERLYGRFSAELSENAMT
                     LRRAGGWREGELWEETSRGSGDPAVSPDGKSLAMVLRDAKGVSKLVVYSTGANEEEAK
                     AEKRIAEMLKRDPEDVAPVRTKPIPRKPEHSYEPADGGGIENPRWTRDGVAILFTHKQ
                     PDLDGLLHHDLFRWTPATGRVERLTHLADVKDADPIDATHAIAVRNRFGASQLVIVDL
                     TTGNVTPRTEPSFDIVYSHPRVGADGRVAWTEHADGEWHVAIDGKRLPAIGAFAPEWG
                     PNGVLIAAVAGRGFIDIAKFDTRRSDSPVRQGLSVGLESPTYVTRVSGMAIDPAPSPD
                     GSLYFMSIEPRGFVVRRLADTSAAPQTKLAFDRSLVPALPPPPATPVALRDEAVTPRD
                     YGLGRQEWQSIFGGQYTAFGHTSEFGIRLGDVAGRLDTILVGAFGSSAMPRGIAIAST
                     WRGWPVELGVHLFDSHDSRGAELRATREMEFPLARVSITGGGLFERNRNRAFADIHAV
                     TWQRRIASERVDVAADSANHAQTMARASMHLGGLTFGAQLGAGRNLALGGPASTIEPD
                     SLLIARVLDPALERNSVTGKSYRAERLSIGTSGVTLFWQRHHLESNIDLLGLESSMSV
                     PPVPIVKTPALQLTAGVARVRVTRKTRAWLTMRWRP"
     gene            8556..9569
                     /locus_tag="VNN25_20085"
     CDS             8556..9569
                     /locus_tag="VNN25_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXH93889.1"
                     /translation="MKRLAILLALLIPFAAHAQEKAPPHIMFVMHGGAGTITRGSMTP
                     DMERQYRETLEQALRTGQGVLMKGGTSVDAVEAGIRVLEDSPLFNAGKGAVFTHEGKN
                     ELDSAIMEGKTKKAGAVAGVTIIRNPISAARAVMEKSPHVMMVGRGAELFATKMGLEI
                     VDPSYFWTERRWKGLQQELLKEQERQANPHAEIKSDDETRHGTVGAVALDTAGNLAAG
                     TSTGGTTNKQYGRVGDSPIIGAGTYADNDSVAVSCTGTGEFFIRWTVAHDIAALYQYK
                     GLTVEQAGNEVIHKKLEPVKGDGGAIILDRKGNFAMPFNSEGMYRGWIGPDGVPHVLI
                     YKE"
BASE COUNT         1900 a         3062 c         3074 g         1729 t
ORIGIN      
        1 tgacctggtc gcgacgcggc tctccggaag tcgtcgtcgc cgtagtgtcg gtcttgaaga
       61 tccctccgcc cagcgtgttg tctcctgacg caggcggcac tggcggcagc ggcggcgcaa
      121 aaccggtcgt cgtaacctcg ggcgagacag gcatcccggc cgtgtcgagc gcgatcgggt
      181 tgccgaattg atcgactgcc ggtgccggtg cggcctgcgg gctatgcagc ggacatggat
      241 tcattggctg cgtgcccgac ttgaacgctt cggaaacgac cgagctcggc gggcaagccg
      301 gtacggccaa gcctccgcta gtctcatcga tctcggccat cgcgattccg tccggcggcg
      361 cgaagtcggc gttcggctgg ccggcggtca ccgactgcat atagcgagtc cagatcggga
      421 cggcgcccga tccacccgaa agcccggtcg gctggttgtc gtcgaatccg atccaggtca
      481 gcgtcagcgt cttcggagtg aatccgatga accaggcgtc acgtttgtcg ttcgtcgtgc
      541 cggtcttgcc cgcggctatg agcttgaatc ccatcgagcg cgtcgccgct gcggtcccgc
      601 ggtcgaccac gcctttcatc acggttagga ggatgtacat gtcgcgtgcg ggaaagacct
      661 gcacggcctg gacgtcgcca ccggcggaca gcacgtgatt cctgtcgtcc atcacgaact
      721 tcacggtgtg caaggggacg cgcgatccca cccgcgcaat cgtggcgtac gcgtcagcca
      781 tctcgatcgg tgcaacgtcg attgcgccga ggagcatcga gggattcttt gagtcgatgt
      841 cggacgtgag gccgagggtg tgcgcagtct tgacgatcgg atcgagcccg cacgccagac
      901 cgatacgcac cgaggcggag ttgagcgact gctccagggc ctgcgccacg gtcacggtct
      961 ggtggtacac atcttcgtaa ttccgcggcg accaatcctc ggagaattgc cgcttgtaaa
     1021 cgaatcgcgt gtcgctcacg agcgtcgccg gcgtgatgtt ctgactcgac aggctcggct
     1081 cgaacgcagt ggcgtagacg aacggtttga ccagtgatcc gatctgacgc ttggcattga
     1141 gcgctcgatt gaattgattg atgtcatagt tgcggccacc gatcaacgcc cgaatctcgc
     1201 cggtcggaac atccacgttg atgagcgcgc cttgcagcgg ttgatcgggc cggcgcaggc
     1261 gcttgctccc cttctccagc gccgaaagac ctgcctcgat ggtgcgtgcg gcgtcgtcct
     1321 gcgcgttcag atcgatggcg gtatagatct gcaggccgcg gcccttgacg ttcttgatgc
     1381 cgtagtcgct ggacatctcc tgcagcacgc ggtcgacgta gtacggaatg gacgacaagc
     1441 cgctgcgttg ccggaacgga cgcggaggca gcttcgtggc catcgctttg tcgtattcgt
     1501 cgcgcgtgat cttcttttga ttgagcatga ggccgagcac cgtcgagcgg cgctgcatgg
     1561 ccaggtccgg acgcgtgaac ggcgagtagt tgttcggact gcggatcatg ccggcaagca
     1621 gcgcggactc ggcgagcgtg agctcggaca ccggcttgcc gaagtagaac cgcgcggcct
     1681 cgccgacgcc gaggatcgag atggatcggt tgcgcccgag gtagatgtcg ttgaggtaag
     1741 cctcgatgat ttctttcttc gaatacttgg cgtcgaggat caccgccatg aacgcttcgg
     1801 tcgcttttcg tttgtaggtc cgctcgttgg tcaggtagta gttctttacc aactgctgcg
     1861 tcagtgtcga tcccccctcg gccacgccac ccgcgcggat attgcgaaac agcgcgcgaa
     1921 acagaccgat cggatcgacg cccgggtgat gccagaaacg aacgtcctcg gtcgctacta
     1981 cagcgtcctg cagagacttc ggcacctgat cgagtgtgac gggacgccgg ttctcgagct
     2041 gatcgctgag gatcgatgtg aggagctccg gttccagcgc cgcgttgtcg atcggcgagg
     2101 cctggcgcag cgagacgacc gagctgatcg caccattctt gaatgcgatg cgcaccggct
     2161 gcgaggggta ctcaccggtt ggatgcgtga acggacgcgt gtagacatcg atctgattgc
     2221 tgcccggtac aaaatcgcca gaccgcgtaa gcgagtccgc ctgacggtaa ccaaggcgga
     2281 tgagcttctc gacgagatcg tcgggagcca gcaccacgcc gccgcgcagc ggggtgaagt
     2341 cggtgaagat tcgcgtcgga agcgaaaggc gccggatctc gaactttccg acggattgtt
     2401 tgtagacgta gagcgtcaag ccgagaatcg agacgaggat caccacaacg gtgatcgaga
     2461 tcgcctggaa gacggtgcgg cggcggggga ggtgagcgtt gaatgtgggc atggtcgaaa
     2521 tcagagatca gagacaagag agcagagatc aggacagaag aatccgacgt ccgggcctgt
     2581 cgggttcaga gatcagagac gaacgacatc agcggctcct gatctctgat ctcttgtcgc
     2641 ttgtctctca tcaccgagcg ataagtgcaa ggttccgtcc acctcgcttg ggatcacggc
     2701 gataggagtg aaagatcgag tcatcgcaac gcgtacagag ccccgagtcg tgaacgttct
     2761 ccggcgcgaa tcctcgctgg atgagtagcg ccgcggtgag tccggcgacg tcgacatgcg
     2821 gtttcgcaca gctgcggtcg acgaacgatt cggcatcgga gtaggcggcg cggagttgat
     2881 cgacgacctc ctcccccacc tcgaagcagc atgctcggat cgtcgggccg agccaggcct
     2941 gggcagtagc ggcatcgaat tccgtctcgc gctcgatcac gtcgagcgtc tccgccacga
     3001 tccgctgcgc tgccccgcgc cagccggagt ggatgttggc gatgacgcga ttcttcgagt
     3061 cagcaatcga gaccggcaga caatcggcaa tcttgatcgc gatggccgcg cctttcgacg
     3121 ccgaccagag cgcgtcgcag gaatcgcact cctgccatcg ttcaccggcg gtcgcattga
     3181 ccgccttctt tccgtggatc tgcgtgcaac tggaaagagt cgcctcgatt ccaaaccgtt
     3241 gatgcacgag acgcatcaac tggttggcga cgctctcgtt gagccgccct tcgaaatcga
     3301 ctgtcgtgta aaagagcccc agccgttcgg gtagcgacgg agggacgacg atacggccga
     3361 tctcgctctg ctcaatgatc cagttcacgg cggcggattg tatgcaaagg ggacgaggta
     3421 gacttctcac gtgccgacga aggttcggga agctgtcagg atgatcgagc aggacgggtg
     3481 ggttcaggtg aaatcgcgtg gcggccatcg tcagttcaag catccaaaga aaccaggacg
     3541 ggtcacgatt tccggcggat caagcgacga catagcgcca ggtacaatga acagcatcct
     3601 caaacaggcg ggcctcaagc gatgaacatg catcggttcc tcatcatcat cgaagacgct
     3661 ggcgcgaatt tctccgccta ctcccccgac cttcccggtt gcgccgcgac cggcaagacc
     3721 cgcagcgatg cggaggcgaa catgtatgag gcaatccaga tgcacatcga aggtctccgc
     3781 gaagacgggt tgcctgttcc ggaatcgacg gcgttcgccg agtacatgct catcggcgct
     3841 gaatcggcgt aagcccggtt acccaatcca ctccgcgatc agcgttccca actccccctc
     3901 ccgtccgctg aagccgtggt cggcgtcgcg catcacgatg agctcagctc gttcgttgac
     3961 gtgttccgaa aggatggcca cgcagcgcgg cacatcgccg aagcagtact cgtcgcgatc
     4021 gccgtagatg tagagcgacg ttttgcggat gccgcggatg tgccggaaaa gcggtcggcg
     4081 ggagagcttc acgccgcgca tcgcttccag gaagggaaag acgttgtagt cgccgttcgg
     4141 attggccata tcgtaaagcg agcgccagct catcatcggc gagccggcat tgcgcggtgc
     4201 cagctcgtcc ccgcgccggg ctttgatcat cgagcgcgat ttgatcaaca tcgcctcgaa
     4261 gcgtcgtttg ccgagttgat cgtagagcaa gccggtatcg tcaccccccg cgatcaggac
     4321 ataacgcttc acgcggttgc gcggtttgta gtagtcgtag acagcgatct tgttcgcacc
     4381 ggtggagtga ccggccagcg tcagatcgcg atatccacgg cgccacagct cgcggatcgc
     4441 gccgtcgacg tcggcgacac agtcgcgaat gcgctcgaat gacgcgccgc cgagggtggg
     4501 gccggcgcgg cgaacgagtc cggagccgcg attgttgaac gggaagaacg cgatgccgcg
     4561 atcgatgaag gtttgcgcaa gaaaattcgt gcggcgcgat tcgaagaccg acccgcctaa
     4621 gccatgcagc cagatgacgg cgcgcttcgt cgctcgcgac ggctcgtaca gaacgccggc
     4681 caacgcaaca tcgtccgccg cctcgaacct tacgaggcgg caggcgggct cggcacgatc
     4741 tggcgtcgtt cgccgtccca tcgccacatc ctttcgcgcg tcaggtacgt cccgccgagc
     4801 cagaagctcg ggttgttgat ctcgacgaag tcgatcttcc cttcaatcat gttgcgcccc
     4861 gccggcgtca gcgtgaagag cgctttcggc ggattcgctt tctcctcccc gatgatcgtg
     4921 atcaccggca cggccgcgcc ggcgagatgt ttaagatgcc gaagaaactc gccatcgccg
     4981 aatccaaagc gcggcggtgc ttgatcgaag cgggtaaaga gcgagacgaa gtcgctggcg
     5041 ccggcatcga tgccttcgac cgcgcgacgc tcgacttcgc cgagtccgtt gcgcgtcgac
     5101 ggaaagcgcg aggcgtgaag cagaaagccg tcgcgaagaa acgggaagtc ggcaacgtcc
     5161 gcgctcagaa agcgattgag cgacgtcgga tcggcgtttg taaacgcgct ccaggccagc
     5221 ttcgccgccg tattcatcgc cggagtgact gcagcgcgcg aatcgaatcg cgcaaagaca
     5281 tcctcctccg attgcgtgcc gagcggctcg gcgcaccaga tgagcgagat gcggcgcact
     5341 ttcgaaagac gcgagaaaag atagagcagg tggacgaggc agaaaagatc gtgctcgaac
     5401 cagaggacga cttcctcttc ctcgtgcgcg cggtcgatga cgttctcctg ctcgagcagc
     5461 tcattgcgaa cccgcaaaag gtgctcgcca tattgctcgg agagaaacgc cgcgcgcgat
     5521 tccacccatt cgtgcagcgg aagagtcggc tgtaccggcc cgccaacaag cgattcgcgg
     5581 aacggcacgt gcctgcccgg aacccccgcc cgccgcgcca gcgaagccga aacatcaccg
     5641 ttgtggaagt gcatcatgcg tcgcggatga tagctgggtt gcgggagtcg cgtttcgggg
     5701 gtcgggagtc ggagtggtca acgccctctg tgaactctgt gtctctgtgg tggtgggaca
     5761 ctcaatagac aacgaaacac agagactcaa ccacagagac actgaggact cagagttgaa
     5821 acttctccga cagccgaccc ccgaaaaccg acccccgcaa ctcacggtct ccatcgcatc
     5881 gtcagccacg cgcgggtttt acgcgtgacg cgcacgcgcg cgacgccggc ggtgagctga
     5941 agcgccggcg ttttcacgat cggcaccggt ggcacggaca tgctgctctc gaggccgagg
     6001 agatcgatat tgctctcgag gtgatggcgt tgccagaaca acgtgacgcc tgacgtaccg
     6061 atcgacaggc gttccgcccg atagctcttg ccggtcaccg agttgcgctc cagcgccgga
     6121 tcgagaacgc gcgcgatcag cagcgagtcg ggctcgatcg tcgatgcggg gccgcccagg
     6181 gcaaggtttc gtccggctcc aagctgagcg ccgaaggtga gcccgccgag gtgcatcgag
     6241 gcgcgcgcca tggtctgcgc gtgattcgcc gaatcggcag cgacgtcgac gcgctcggaa
     6301 gcgatgcggc gttgccaagt gacggcatgg atgtcggcga acgcccgatt gcggttccgc
     6361 tcaaacagcc cgccgccagt gatggagacc cgcgccagcg gaaactccat ctctcgcgtc
     6421 gcccgcaact ccgctcctct gctatcgtgc gaatcgaaca gatgcacgcc gagctcgacc
     6481 ggccatccgc gccatgtcga cgcgatcgcg atcccgcggg gcattgcact gctgccgaat
     6541 gcaccaacta ggatcgtatc gagtcgcccg gcgacgtcgc cgaggcggat cccaaactcg
     6601 gacgtgtggc cgaacgcggt gtactgacca ccgaagatcg actgccactc ttgacgcccg
     6661 aggccgtagt cgcgcggcgt cactgcttcg tcgcgcaagg caacgggcgt ggctggcgga
     6721 ggcggaaggg cgggaacgag cgatcgatcg aatgccagct tcgtctgcgg agccgcactc
     6781 gtatccgcga gtcgccgcac gacgaaacct cgtggctcaa tcgacatgaa atagagcgag
     6841 ccgtccggcg acggcgcagg atcgatggcc ataccggaga ctcgtgtgac gtaggtcgga
     6901 ctctctagtc cgactgagag accttgtcgg actggagagt ccgacctacg tgtatcgaac
     6961 ttcgcgatat cgataaaccc gcgcccggcg acggccgcga tcaacacgcc gttcggtccc
     7021 cactccggtg cgaatgcgcc gatcgcgggc agacgctttc catcaatcgc gacatgccac
     7081 tctccgtccg catgctcggt ccaggcaaca cgtccatccg cgccgacgcg gggatgcgag
     7141 tagacgatgt cgaacgacgg ctccgtgcga ggcgtgacgt tgccggtcgt caagtcgacg
     7201 atcacgagtt gcgaggcgcc gaatcgattg cgaaccgcga tcgcgtgcgt ggcatcgatc
     7261 ggatcagcgt ccttcacgtc ggcgaggtgc gtcagtcgct cgacgcgccc cgtcgccggc
     7321 gtccagcgga agagatcgtg atgcagcaag ccgtcgaggt cgggctgttt gtgcgtaaag
     7381 agaatcgcca caccgtcgcg cgtccagcgc ggattctcga tgccgccgcc atctgccggc
     7441 tcatacgaat gctccggctt gcgcggaatg ggctttgtcc gaacaggcgc cacgtcttcc
     7501 ggatcgcgct tcaacatttc ggcaatccgc ttctccgctt tcgcttcctc ttcgttcgct
     7561 ccggtcgaat agacgaccag cttcgagaca cccttcgcat cgcgcaacac catggccagg
     7621 ctctttccgt cgggtgagac ggcaggatcg ccgctgcctc gcgatgtctc ctcccacaac
     7681 tccccttcac gccagccgcc ggcgcgtcgg agcgtcatcg cgttttccga cagctccgcg
     7741 gaaaagcgcc cgtacaaccg ctccggcgaa tcgccgaaca cgcccgtgaa ggcctcgtcg
     7801 aaactgcgcc gccggcgtgc ggtcatccgt gcccagagat ggcgcagcga atccggtccg
     7861 ctcttctcgg ccaaccattc gagaaacgcc gagccggcga ggtaggccat cgatccgccg
     7921 aggaagcgag gatcggaatc gagttggtcg tacttcggca actcaccgtt catcgcccac
     7981 atccgaagca ccgccgcgcg catcgcaccc gagggacgtc cgctgccggt gatgcgccct
     8041 tcgatcaccg ttgcgtatcc ctccagcacc cagcgcggcg cgtgaagcgt gatcggattg
     8101 agcgggagca aatgcgccat cgcccgctcg aacggattac gcgacggccg cagcagatgg
     8161 acgagatgcg cggtctcgtg gacggtcagc aagtcgatcc agttcgaata ctcaccgatc
     8221 acttcctccg gccctggcgc ctccggatac agcacaatcc tcggcgtatc caacagcgga
     8281 atagtcgcgc cgttcgcctc cgcgatcggg ttctcgacga tgacatccgt taccgtctcc
     8341 ggcgcaaacc ccacctcggc caccaccgcc gtgcgcaccg actcgagccg cgacgctgcg
     8401 cggatcgacc acgcttcgta ctgcgccgga tagtgaacgc gaaagtgtgg cgtcgtgatc
     8461 gtgcgccagg ctccatgagg cgcctgcgcg acagccgccg tcgcgaggag gagcgcgatg
     8521 aacgcgaacc cgcggatcat gcaaaaatgc gcctcatgaa acgacttgcg atcctgctcg
     8581 ccctgctgat tccgttcgcc gcccacgccc aggaaaaggc gccgccacac atcatgttcg
     8641 tgatgcacgg aggcgcggga acgatcacgc gcggcagtat gacgccggac atggaaagac
     8701 aatatcgcga gacgctggag caggcgctgc gaaccggcca gggtgtactc atgaaaggcg
     8761 gcacgagcgt cgacgccgtc gaagcgggca ttcgcgtcct cgaagactct ccgctcttca
     8821 acgccggcaa aggcgcggtc ttcacgcacg agggaaagaa cgagctcgac tccgcgatca
     8881 tggaagggaa gacgaagaag gcaggtgccg tcgccggggt gacgatcatc cgcaatccga
     8941 tctccgcggc ccgcgcggtg atggagaagt ccccgcacgt gatgatggtc ggccgcggcg
     9001 cggagctctt cgccacgaag atgggactcg agatcgtcga cccgtcgtac ttctggaccg
     9061 agcgccgctg gaagggcctg caacaggagt tgctgaagga gcaggagcgc caggccaatc
     9121 ctcacgccga gatcaagtca gacgatgaga cccgccacgg cacggtcggc gcagtcgcgc
     9181 tcgatacggc tggcaacctc gccgcgggaa cgtccaccgg cggcacgacg aacaagcaat
     9241 acggccgcgt cggcgactcc ccgatcatcg gcgccggcac ctacgccgac aacgactccg
     9301 tagccgtctc ctgcaccggc accggcgagt tcttcatccg ctggaccgtc gcccacgaca
     9361 tcgccgccct ctatcaatac aaaggcttga ccgtcgagca agccggcaac gaagtcatcc
     9421 acaagaaact cgaacccgtc aaaggcgacg gcggcgcgat catcctcgac cgcaaaggca
     9481 acttcgccat gccgttcaac agcgaaggta tgtaccgcgg atggatcgga cccgatgggg
     9541 tgccgcatgt gttgatttat aaggagtgat ggcttatcgc aggagggtaa gctcgttcaa
     9601 cgctcttcgc cgcgaagtgt cctgacgcgt tcgaagaacg ttttccagtc gtggacttga
     9661 tatctagttg cgaagttgcc tttcggcaga ccggccgcga agaacttatc ctgcacgtct
     9721 ctgtcaattc gtaccgtcag aacgagcact tccacctgct tttgc
//
LOCUS       DATLVW010000691         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_440145, whole genome shotgun sequence.
ACCESSION   DATLVW010000691 DATLVW010000000
VERSION     DATLVW010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440145"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VNN25_20090"
     CDS             <1..650
                     /locus_tag="VNN25_20090"
                     /inference="COORDINATES: protein motif:HMM:NF024041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93890.1"
                     /translation="HKNEILKASAPRVSEEILDLLRRGWSRGAVQLMVDTGLLDPLLP
                     EVYKSIAGDRAPYFWKMLEVLDRTVQAGRRISDPVLLSVMFLPWILQELETEETRRTT
                     KMKTSDVVVFIRELIQPMCTRMALAAGTRHQIETALDTLWRLLEPPVDRKAQIRFVFR
                     EPFNDALALLELYALSSGKYVEVFRQWREFAQRARRVADSEATPLPSAKRRRRRR"
     gene            792..1916
                     /locus_tag="VNN25_20095"
     CDS             792..1916
                     /locus_tag="VNN25_20095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXH93891.1"
                     /translation="MPVYMDHHATTPVDPRVVETMLPYFTEFFGNPASLTHAYGKRAS
                     IEVEDARGVIAKFFGVAQNEIYFTAGATESNNIALNLLAPGDHAITSAMEHKSILVPL
                     QRLGKHGVEVTMLTPDAEGFIDPEELRGAIRPNTKLVSIEAANGEIGTLQPIGALGAI
                     CRERGVLFHSDITQGAGKVPLDLTNVDLAAFSAHKLYGPKGIGGMIVRRGIRIEPVML
                     GGGQEKGARSGTVNVPGVVGMARAFWFREKEMAAEGERLTALRNELWDRVVDEVPGAF
                     VNGPREKRLPGNLNVAFERVESDAVMLALRHFALSSGSACSSGDRSASPVLTAIGVAA
                     ELAYGSIRFGLGKSNTTEHVALLVDDLKRNIPRLREISAA"
     gene            1913..2617
                     /locus_tag="VNN25_20100"
     CDS             1913..2617
                     /locus_tag="VNN25_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXH93892.1"
                     /translation="MSTPEPPRILLVDQSRGSLLFQESILRRREAMIVTALTGSEALE
                     IARTDRPQLIMFAFDLFDMNAPEFCREIRADAATRTMSLLLVCERDSPEHADLCLSAG
                     CNDVIYRPLERMELDGKVARLTTIPSRRELRTLTRIEVSVENNGRFIIGRSINVSANG
                     MFLEVDRVLPSEGKIRLHFYLPADPKPLQLDCEVLRAEFAGSAAKYGVRFVSLGDGER
                     ERIERYVRRLRSRELL"
BASE COUNT          475 a          845 c          865 g          481 t
ORIGIN      
        1 ggcacaagaa cgagatcctg aaagcctcag cgccgcgcgt ctcggaagag attctcgatc
       61 tgctgcggcg cggttggtcg cgcggcgccg tccagctcat ggtcgacacc ggtcttctcg
      121 atccgctgct ccccgaggtc tacaaatcga tcgccggaga tcgggctccg tacttctgga
      181 aaatgctgga agtgctcgac cgtacggttc aggcgggacg caggatttcc gatccggtgc
      241 tgctgtcggt gatgttcctg ccgtggattc tgcaggagct cgaaacggaa gagacgcgcc
      301 gtacaacgaa gatgaagacg agcgacgtgg tcgtcttcat ccgcgagctg atccagccga
      361 tgtgtacgcg gatggcgctg gcggcaggaa cgcggcacca gatcgagacc gcgctcgata
      421 cgttatggcg cctgctcgaa ccaccggtcg atcgcaaggc gcagatccgt ttcgtcttcc
      481 gcgagccgtt caacgacgcc ctcgcgctcc tcgagcttta cgcgctctca tcaggcaagt
      541 acgtcgaggt cttccgccag tggcgcgagt tcgcccagcg cgcccggcgc gtggcggatt
      601 ccgaagcgac accgctgccg tctgcgaaga gaagacggcg aagaaggtag ttcccgccca
      661 gggaagagag acctttttca cgcggagacg cggagggcgc ggagttgttc tccgctcctc
      721 cgcggctccg cgtgacagcc gttggttctc cctgagcctc gtgacagcgg tatcattctc
      781 acgtgcccga aatgccggtg tacatggacc atcacgccac gacgccggtc gacccgcgcg
      841 ttgtcgagac gatgctgccc tacttcaccg agtttttcgg gaatccggcg tcgctgacgc
      901 acgcgtatgg caagcgggcg tcgatcgaag tcgaagatgc ccgaggagtc atcgcgaagt
      961 tcttcggcgt cgcgcagaat gagatttact tcactgccgg agcgacggaa tcgaacaaca
     1021 tcgccctcaa tctcctcgcc cccggcgatc acgcgatcac ctcggcgatg gagcacaagt
     1081 cgatcctcgt gccgctgcag cggctcggga agcacggcgt cgaggtcacg atgctgactc
     1141 cggacgccga ggggttcatc gatcctgagg agctccgcgg cgccatccgc ccgaacacga
     1201 aactcgtctc gatcgaagcc gcaaacggcg agatcggtac gctgcagccg atcggcgcac
     1261 tcggtgcgat ctgccgcgag cgcggcgttc tctttcacag cgatatcacg cagggggccg
     1321 gcaaagtgcc gctggacctg acgaatgtcg atctggccgc attctccgcg cacaagctgt
     1381 acggtccgaa gggaatcggc ggcatgatcg tgcgtcgcgg catccgcatc gagccggtca
     1441 tgctcggcgg cggccaggag aagggcgccc gcagtgggac ggtgaacgtg ccgggcgtcg
     1501 tcggcatggc tcgggcgttc tggtttcgcg agaaggaaat ggccgccgag ggcgagcggc
     1561 tgacggcgct gcgcaacgag ttgtgggacc gcgtcgtcga cgaagtgccc ggcgcgttcg
     1621 tgaacggacc acgcgagaag cgtctgcccg gcaacctcaa tgtcgcgttc gagcgcgtcg
     1681 aatccgacgc ggtgatgctg gcccttcgcc attttgcgct gtcgtcgggc tcggcgtgca
     1741 gttcaggaga ccgcagcgct tcgccggtgc tgacggcaat cggcgtcgcc gccgagctgg
     1801 cctacggctc gatccgcttc ggcctcggca agtcgaacac gaccgagcat gtcgccctgc
     1861 tcgtcgacga tctcaaacgc aacattccac gccttcggga gatctccgcc gcgtgagcac
     1921 ccccgagcct ccgcgcatcc ttctggtcga ccagtcgaga ggctcgctgt tgtttcagga
     1981 gtcgattctt cgccggcgcg aggccatgat cgtgactgca ctgaccggaa gcgaggcgct
     2041 ggagatcgcg cgcacggacc ggccgcagct catcatgttc gccttcgatc tcttcgacat
     2101 gaacgcaccc gagttttgcc gcgagatccg cgcggacgcc gcgacgcgca cgatgtcgct
     2161 gctgctcgtc tgcgagcgcg attcgcccga gcacgccgat ctttgtctct ccgccggttg
     2221 caacgacgtc atctaccggc cgctggagcg aatggagttg gacgggaagg tggcgcggtt
     2281 gaccaccatc ccatcccgcc gcgagctgcg gacgctgacg cgcatcgaag tctcggtcga
     2341 gaacaacggc cgtttcatca tcggccggag catcaacgtc tctgccaatg gcatgtttct
     2401 ggaagtcgat cgcgtgttgc cgtccgaggg aaagatccgg ctgcatttct atctcccggc
     2461 ggatccgaaa ccgttgcaac tggactgcga agtgctgcgc gccgagtttg cgggaagcgc
     2521 cgcgaaatac ggcgtccgct tcgtcagcct cggtgatgga gaacgcgaac ggatcgagcg
     2581 ctacgtgcgg cggttgcgct cgcgtgagct gctgtgatga tcgacgcaca acaaaagagc
     2641 ctggacgaag cgatcgagct gactcg
//
LOCUS       DATLVW010000692         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_565282, whole genome shotgun sequence.
ACCESSION   DATLVW010000692 DATLVW010000000
VERSION     DATLVW010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565282"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VNN25_20105"
     CDS             complement(<1..388)
                     /locus_tag="VNN25_20105"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HXH93893.1"
                     /translation="MSHNLSLRILSLFLAFTVWFFVSAPRREPVSERAFAAPVSIVRM
                     PRDLVITTPVPDTVSVRLRGRISDLRSLSSQNLEVTLDVSWVTPGDAVITLSPQAMSV
                     PPQIDVVSIEPMKLRFHVEALRQTVVP"
     gene            complement(543..1304)
                     /gene="cdaA"
                     /locus_tag="VNN25_20110"
     CDS             complement(543..1304)
                     /gene="cdaA"
                     /locus_tag="VNN25_20110"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXH93894.1"
                     /translation="MSWYDRILALQFSWRDALDIVIVAFVIYNILALIRGTRAMQVSI
                     GMVLLGSTFFVARAFNLPALEAISRQILFYLPFAVIVLFQAEIRRALARMASNPLLSL
                     FPSGTSPVPFDVILDAATVLSANRIGALIAIERAQSLKMYSEAAKPIDALASAELLIS
                     IFTPGGPLHDGAAIVRGNRIVAAGAFLPLTTSADPKLAHGTRHRAALGLSEDSDALVI
                     VISEENGSIAATSEGVLHEHLDSDQLLQLMQERLK"
     gene            complement(1786..2634)
                     /gene="folP"
                     /locus_tag="VNN25_20115"
     CDS             complement(1786..2634)
                     /gene="folP"
                     /locus_tag="VNN25_20115"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXH93895.1"
                     /translation="MNTGRVTDRSSFIVNSSSSPAVMGILNVTPDSFSDGGVHLDHTK
                     ALHAALQMEADGAAIIDIGGESTRPGAEPLAAQIEIDRVVPVIEQIRKRSAIQISIDT
                     RKPEVAEEAIRAGANIVNDVSGMRYSAAMPAVVAGNGVPVILMHMRGEPATMQELAVY
                     DDVVADVARELISIIDDAVAAGVDRNKILIDPGIGFAKTFDQNLELLARADELTSLGP
                     VVIGASRKAFIGHLTGRTSGPERAIGSLAAVAAAHLAGAAIVRVHDVRETVDFLKVWM
                     AIEGRR"
     gene            complement(2664..>3194)
                     /locus_tag="VNN25_20120"
     CDS             complement(2664..>3194)
                     /locus_tag="VNN25_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXH93896.1"
                     /translation="EDAVPEHMYAVRYMLEKVGIDIKIEGKDIRVPKDQALRVRYDIG
                     AAVPKIDDGPWPLFPADLLSIMIIIATQAEGTVMIFEKMFESRLFFTDKLIQMGARII
                     LCDPHRAIIVGGNRLSGAEISSPDIRAGMALLLAALCAKGESVIHNIHQIDRGYERIE
                     ERLNPLGARIRRERDS"
BASE COUNT          580 a          963 c         1059 g          592 t
ORIGIN      
        1 tggggacgac ggtctgccgc agcgcttcca cgtgaaagcg gagtttcatc ggctcgatcg
       61 acacgacgtc gatctgcggc gggacgctca tcgcctgcgg gctgagcgtg atgacggcat
      121 cgcccggcgt tacccagctc acatcgagcg tgacttcgag attctgcgat gagagcgatc
      181 gaaggtcaga gatccgtccg cggagacgaa cgctgaccgt gtccggaacg ggcgtggtga
      241 taactaggtc gcgcggcatg cggacgatcg atacaggcgc cgcgaatgcg cgctccgaga
      301 ccggctcccg ccgtggcgca gaaacgaaga accacaccgt gaacgcaagg aacagggaga
      361 ggatgcggag cgagaggttg tggctcatgc tgcgtctccg gcagcctggc ggctgcctcc
      421 gacgcgttgt cggttgtcgg ttggttgcgc cttcggcgcg ttagtaagaa cgcgcggcga
      481 ggtttttttc aacgcgccga aggcgcatct aaccgacaac cgacaacgcg ccgcaggcgc
      541 aatcatttca gccgctcctg catcagttga aggagttgat cgctgtcgag gtgctcgtgc
      601 aggacgcctt cgctcgtggc ggcgatcgaa ccattttctt ccgagatcac aatgaccaac
      661 gcatcgctat cctcggatag ccccagcgca gcccgatggc gcgttccgtg ggcgagcttc
      721 ggatcggcgg atgtggtgag cggaaggaac gcgcccgcgg cgacgatgcg gttgcctctc
      781 acgatcgcgg caccgtcgtg aagcggcccg cccggcgtga agatgctgat gagcaattcg
      841 gcggaggcga gagcgtcgat cggtttcgcc gcttccgaat acatcttcag gctctgcgcg
      901 cgctcgatgg cgatcagcgc gccgatacgg ttggcagaga gcaccgtagc cgcatcgaga
      961 atgacgtcga acggaacagg agacgttccg ctcggaaaga gcgagagcag cgggttggac
     1021 gccatgcgcg ccaacgctct gcggatctcc gcctgaaaca gcacgatgac ggcgaaggga
     1081 agataaaaga gaatttggcg ggagatcgct tcgagcgcgg gcaggttgaa ggcgcgcgcc
     1141 acgaagaacg tgctgcccag caacaccatc ccgatcgaaa cctgcatcgc ccgcgtgcca
     1201 cggatgagcg cgaggatgtt gtagatgacg aaggccacga tgacgatgtc gagggcatcg
     1261 cgccacgaga actggagggc caggatacgg tcgtaccaac tcactggatt gcgcctccgg
     1321 cgcgttgtcg gttgtcggtt ggttgcgcct tcggcgcgtt gtcagagggc caagcgtgca
     1381 gtcttgccag ctcggcgaag cggcacgcgt ttcttccact cttttctgga gtccggcact
     1441 tcccgtcgag cgccttctcc ccccgcggtt tttgcggggg gagaaggtgc cgaaggcgga
     1501 tgagggggga tttgattagg cgtggagagc tttcgtctgt gaccctcgaa aagctggcca
     1561 agcggtttaa atgcatcctt gacgcatcga tgaacgaccc cctcatccgg ccatcggcca
     1621 ccttctcccc cgctgagaag cgcgggggcg aaggcgctcg atgggagggc gttccagtga
     1681 ttcgagttag cgctgagaat tgcgagccaa gcgcccaatc gcgcgggttt gccaacgcgc
     1741 cggaggcgca atcaaccgac aaccgacaac gcgccgcagg cgcagtcatc gccttccctc
     1801 gatcgccatc cataccttca ggaagtcgac ggtctcgcgc acgtcatgca cgcggacgat
     1861 cgcggcgccg gccaggtgcg ctgcggccac cgccgcaagc gaaccgatgg cgcgctcggg
     1921 gccggatgtg cggccagtca gatgaccgat gaatgccttg cgtgaggctc cgatcacgac
     1981 cggcccgagt gaggtcagct cgtccgcgcg ggccaatagt tccaggttct ggtcgaaggt
     2041 cttggcgaag ccgatgccgg ggtcgatgag gatcttgttg cggtcgacgc cggcggcgac
     2101 agcatcgtcg atgatcgaga tcagctcgcg ggccacgtcc gccaccacgt cgtcgtacac
     2161 cgccagttcc tgcatcgtcg ccggctcgcc gcgcatgtgc atcaggatca ccggcacgcc
     2221 gttgccggca acgactgctg gcattgccgc cgagtagcgc atgccgctga cgtcattgac
     2281 gatgttcgct cccgcgcgga tggcctcttc tgccacctca ggcttgcgtg tgtcgatgga
     2341 gatttggatt gcgctgcgct tgcggatctg ctcgatgacc ggaacgacgc ggtcgatctc
     2401 gatctgcgcc gccagaggct ccgcgccggg ccgggtcgat tcgccgccaa tgtcgatgat
     2461 tgctgcgccg tcggcttcca tctgcagcgc tgcatgcagg gccttggtgt ggtcgaggtg
     2521 aacgccgccg tccgaaaacg agtcgggcgt tacgttgagg atgcccatga cggcgggcga
     2581 ggatgagcta ttaacgatga aggacgaacg gtcagtaact ctcccggtgt tcatcgttca
     2641 tccttcctca tgcatcctgc ggattacgaa tcccgctccc gcctgatcct cgctcccaac
     2701 ggattcagac gctcctcgat ccgctcgtag ccgcggtcga tctggtggat gttgtggatc
     2761 acgctctcgc ctttggcgca gagtgcggcg agcagcagag ccataccggc gcggatgtcg
     2821 gggctggaga tctccgcgcc gctgaggcgg ttgccgccga caatgatggc gcggtgggga
     2881 tcgcagagaa tgatgcgcgc gcccatctgg atcaatttgt ccgtgaagaa gaggcgggac
     2941 tcgaacatct tctcgaagat catcaccgtt ccctcggcct gcgtggcgat gatgatcatg
     3001 atcgagagca gatcggcggg gaaaagcggc catggtccgt cgtcgatctt cggcacggcc
     3061 gcgccgatgt catagcggac gcgcagcgcc tgatccttcg ggacgcggat gtccttccct
     3121 tcgatcttga tgtcgatacc caccttttcg agcatgtagc ggacggcgta catgtgctcg
     3181 ggcacggcgt cttc
//
LOCUS       DATLVW010000693         3339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_73698, whole genome shotgun sequence.
ACCESSION   DATLVW010000693 DATLVW010000000
VERSION     DATLVW010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73698"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VNN25_20125"
     CDS             <1..443
                     /locus_tag="VNN25_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH93897.1"
                     /translation="RPELRIAAIEALAKAGIPAGVMVAPVVPAITDHEMPAILTAAAN
                     AGGTSAGFVVLRLPWAVAPLFEKWLEEHYPDRKEKVMNRVRDLREGKLYDPQWGVRQR
                     GKGIFADQIASMFEVTCRRLKLDEKKYDLSTDAFRRPTTQGSLF"
     gene            446..967
                     /locus_tag="VNN25_20130"
     CDS             446..967
                     /locus_tag="VNN25_20130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXH93898.1"
                     /translation="MSNEAKTVFEGKHVLVLERDTWQYVERKKGKTAVIILAVTDDDR
                     VLFVEQYRRPLNARVIEFPAGLVGDEEGHDDPEETARLELEEETGYTCRTVERLTSGP
                     TSAGITSETVTFFRASGLEERGQGGGIGSEDITVHRVPRNAVVDWLKRKNGEGILIDA
                     KVWAGLWWLGIGQ"
     gene            complement(1020..2171)
                     /locus_tag="VNN25_20135"
     CDS             complement(1020..2171)
                     /locus_tag="VNN25_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93899.1"
                     /translation="MAKESRWPLMAAIAVLIVHGCVVFRSGVSLPPDSVAFSLRADLL
                     IANHFNFPAASRATGVHNVPAGMYMLFTTLVALAKLVAGARWPTILVVANLCFDALTA
                     AILVKLVRLATRSNAAAVLAIVVWLLCYDVVTWVRMPLTDVPFLVVSFAAFASVVAPY
                     LAGEKPTRKNIIASVILTVISVLLRPVGFLWLILMVTASLVMSGRLRRRSLIASALLV
                     AATVFLAHTFVVRNPESWTIETLARSVRWDAKSYRLGEVIKGRPETFHAPPSTAVDYA
                     AITADRFVHFFAPMVATFSRGHKIAGVVFYAPLYLLAIAAVIAAARRKGTTADVVILS
                     AVVVLTVAFWHSLVVIDYDWRYRLPILPHLVFLAACAVPMLLRNRAIAE"
     gene            complement(2177..>3339)
                     /locus_tag="VNN25_20140"
     CDS             complement(2177..>3339)
                     /locus_tag="VNN25_20140"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXH93900.1"
                     /translation="LELDLPYTSMPEAIYELFIKGKHSIVVTGTHGKTTTTSLMAWLL
                     ESAGRDPSMVVGGIPLNFNHNFKLGDGREFVIEGDEYNTSFFDKGPKFLHYGANTLLV
                     NNIEFDHADIYANLDAIIDAFRKGVQQVAPGDMIVANGDDANVRSLRTDARARWFTFG
                     TSRSCDIFATDPEYTPAGSAFTAWWEGSEWARFRTTMSGAHNVVNALADIAIARHRGL
                     SAEEIQRGLETFEGIKRRMEVRGVERGITVIDDFAHHPTAIDTTLAGARRRFPGQRIW
                     ALFEPRSISSSRKEFEPGYIEAFHQADRVIIGRVFHKGRYETRYGLDKMMSVETIVER
                     LNADGVRAEQIDDVDAIAGVVARDAQPDDVVLVMSSGSFDGVHEKILERLRE"
BASE COUNT          694 a         1000 c         1082 g          563 t
ORIGIN      
        1 cgcgtccgga gctgcggatc gcggcgatcg aagcgctggc gaaggcgggg attcccgcgg
       61 gtgtgatggt ggcgccggtc gtgcctgcga tcacggatca cgagatgcct gcgatcctga
      121 ccgcggccgc gaacgccggc ggaacatcgg ccggattcgt cgttctgcgt ttgccgtggg
      181 ctgtggcgcc gctgttcgag aaatggctcg aagagcacta tcccgaccgc aaggaaaagg
      241 tgatgaaccg cgtccgcgat ctgcgcgaag ggaagctcta cgatccgcag tggggcgtcc
      301 gccagcgagg caaagggatc ttcgccgatc agatcgcgtc gatgttcgaa gtgacatgcc
      361 gccgcttgaa gctcgatgaa aagaaatacg acttgtccac ggacgctttt cggcggccta
      421 cgacgcaagg tagtctcttc tgatcatgag caacgaggcg aaaaccgtct tcgaaggaaa
      481 gcacgtcctg gtgctggaac gggacacatg gcagtacgtc gaacggaaga agggaaagac
      541 agcggtgatc atcctcgccg tcaccgacga cgatcgcgtg ctcttcgttg agcaataccg
      601 ccgccctctc aacgctcgtg tcatcgagtt tcccgcagga ctggtcggtg atgaagaagg
      661 ccacgacgat cccgaggaga ctgcaagact ggagttggaa gaggaaacgg gctacacgtg
      721 caggacggtc gagcggctga cgagcggccc gacaagtgcc ggcatcacct ccgagaccgt
      781 gacgttcttt cgtgcttcag ggctcgaaga gcgcggccag ggcggcggca tcggcagcga
      841 agacatcacg gttcaccgcg tcccgcgcaa cgccgtcgtt gattggttga agcgcaagaa
      901 cggcgaagga atcctgatcg acgcgaaggt gtgggcggga ttgtggtggc tcggcatcgg
      961 ccagtgaagg agtgcggacg tctcgtccgc ggccgccggg cgtcccgccc ggcggtggtt
     1021 tactccgcaa ttgcccgatt ccgcaacaac atcggcaccg cgcaggcagc caggaaaacc
     1081 aggtgaggca ggatcggaag ccggtagcgc cagtcgtagt cgatgacgac gagcgaatgc
     1141 cagaacgcca cagtcagcac gacaactgcc gacagaatca cgacatcggc ggtcgtccct
     1201 ttgcgtcgcg ctgccgcgat caccgcggcg atggccaaaa ggtatagggg cgcgtaaaac
     1261 acaacaccgg cgatcttgtg accgcggctg aatgtagcaa ccattggcgc gaagaaatgt
     1321 acgaaccgat ccgcggtgat cgcagcgtaa tcgacagcgg tcgaaggcgg cgcatgaaat
     1381 gtctcgggcc tccctttgat gacctcgccg agccggtagg acttcgcatc ccaccgcacg
     1441 ctcctggcca aggtctcgat cgtccagctc tcgggatttc gcacgacgaa cgtgtgcgca
     1501 aggaacaccg tagccgcgac gagcagtgcc gatgcgatca ggctgcgcct ccgcaaacgg
     1561 cccgacatca cgagcgaagc ggtcaccatc aaaatcagcc agagaaagcc aaccggacgg
     1621 agcaggactg agatcacggt caggatgacg gaggcgatga tgttcttgcg ggtcggcttc
     1681 tcgccagcaa ggtacggagc taccaccgaa gcaaaggcgg cgaagctaac gaccaggaag
     1741 ggcacgtccg ttagaggcat tcgcacccat gtcacgacgt cgtagcagag cagccagaca
     1801 acgattgcga gtactgcggc ggcgttggag cgcgtggcga gacgaaccag cttgacgaga
     1861 attgccgcgg tgagggcatc gaagcagaga ttcgcgacaa caaggatcgt tggccatctc
     1921 gcgccggcga cgagtttggc aagcgccacc agcgtcgtga agagcatgta cattcccgcc
     1981 ggcacgttat ggacgccggt ggcacgtgac gccgcaggga agttgaagtg attggcgatc
     2041 agcaggtcgg ctcggagcga aaacgcgacg ctgtccggcg ggagcgagac gccggaccga
     2101 aacacaacgc aaccgtgaac gatcaggacc gcgattgccg ccatgagcgg ccagcgcgac
     2161 tccttcgcca tcgcgctcac tcgcgcagcc gctcgagaat cttctcgtgc acgccgtcga
     2221 acgagcccga tgacatgacg aggacgacgt cgtccggctg ggcatcgcgg gcgaccacgc
     2281 cggcgatggc gtcgacatcg tcgatctgct cggcgcggac gccatccgca ttcaggcgct
     2341 cgacgatcgt ctcgacagac atcatcttgt cgaggccata acgcgtttcg taacgccctt
     2401 tgtgaaagac gcgcccgatg atgacgcggt ccgcctgatg aaatgcctcg atgtatccag
     2461 gctcaaattc tttgcggctc gaggagatcg aacgtggctc gaaaagggcc cagatgcgct
     2521 ggccggggaa ccgccgcctc gcgccggcga gcgtcgtgtc gattgctgtg gggtgatgcg
     2581 caaagtcgtc gatgaccgtt atgccacgct cgacaccgcg cacttccatc cgccgtttga
     2641 tgccttcgaa agtctccagc ccgcgctgaa tctcctcggc ggagagtccg cgatgccggg
     2701 cgatggcgat gtccgccaac gcgttgacga cgttgtgcgc acccgacatg gtcgtgcgga
     2761 agcgcgccca ttcgctgcct tcccaccacg ctgtgaacgc gctgccggcc ggcgtgtact
     2821 cgggatcggt ggcgaaaatg tcacagctac gcgacgtgcc gaacgtgaac caccgcgccc
     2881 gcgcatcggt ccgcaacgag cgcacattgg cgtcgtcgcc gttcgcgacg atcatatccc
     2941 caggcgcgac ctgctgcaca cctttgcgga aggcatcgat gatcgcgtcg aggttcgcgt
     3001 agatgtcggc gtgatcgaac tcgatgttgt tgaccagcag cgtgttcgca ccgtagtgca
     3061 agaacttcgg tcccttgtcg aagaacgagg tgttgtactc gtcgccctcg atgacgaact
     3121 cacggccgtc cccaagcttg aagttgtgat tgaagttgag cgggatgccg ccgacgacca
     3181 tgctcggatc gcggccggcc gactccagca gccaggccat gagcgaggtc gttgtcgtct
     3241 tcccgtgcgt gccggtcacg acgatggagt gttttccttt gatgaacagc tcatagatcg
     3301 cctccggcat cgatgtgtaa ggcagatcga gctcgagcg
//
LOCUS       DATLVW010000694         4501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_28584, whole genome shotgun sequence.
ACCESSION   DATLVW010000694 DATLVW010000000
VERSION     DATLVW010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28584"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..2229)
                     /locus_tag="VNN25_20145"
     CDS             complement(22..2229)
                     /locus_tag="VNN25_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93901.1"
                     /translation="MIVLLLLGIVLPVVLLVASVRRWIAPVPWTIALLLMMLTLVFLN
                     GAVFSSRLPVPVDEVARGYPWRGLFGDVVAANPLTNDTVKLFLPWMQVAREELAHGRA
                     PLWNRYSFAGYPLLANGESAPFSPLFLATLIVPLPKQIVAMAAMKIFAALLFTYLFLK
                     REKASDAAAVFGAIVFGFSTVMTVFLYYSTASVVAFLPAAAFALLHAIDRQTKASIVL
                     VALVIATLLANGHPESVLHIAVGCGGILLIEALAVRSDFGVRDFLRRLLAPLLGVAAG
                     LAVGAPAWVPVLEQVRISERFDTLRKVAPVPMQPFTAAWAIISPNGFGNPVRHNWSWM
                     QHYVGVAISYAGLLPLALALAVVIARRTEARERWRIALAIVLFFVAMDWTIVGHALNA
                     IPPFNIVANDKLRFVCIFLIAIVAAKALDVSKTAMAIASAPIFLLAIYVYTKRSDLMR
                     PVDLIGVAAILIFLFLPQRFRSWAAVVLVFTELCAFNSGFNALVDAKYFRPPLPIIDA
                     LRAHAPREPFRVAGLAWVLLPDSSAQYGLEDVRGSDPMAFASYDSYLRRFTEPDLGWL
                     PLIKDANRPELNFMNVRFLFGEPDSLPGGAWRLLYRGADGTLWENPSVLPRFFAQGAE
                     VRNLRSGAPAEFAMTVVAPSPTMVQSSEVLAPGRRVYVGGRRVPLRHIEGTFIGFEVP
                     AGASDVRVVYRPMSFYGSCAVAVLAVLVLWFWPVRKVAAEPAAEQPSGRRRYV"
     gene            complement(2466..2717)
                     /locus_tag="VNN25_20150"
     CDS             complement(2466..2717)
                     /locus_tag="VNN25_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93902.1"
                     /translation="MPRPPSYPLPLVAGSKRPSRLRCELCFHIGAAVRVGARLLCGAC
                     FHDVALSEEAARGERGAVSASLRSTDAFHAVADGFDAVE"
     gene            complement(2792..3340)
                     /locus_tag="VNN25_20155"
     CDS             complement(2792..3340)
                     /locus_tag="VNN25_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93903.1"
                     /translation="MKKTITVVLALMMAATLAVANDHGAPPAGPGGPGGPGHGVEGDG
                     HLSIAADGTVVVVRAAASSTTSTPVAEVIAIRGGAIAWSATLPSPHADVQISGSQVIE
                     VTDTTASGASSPTTTLTALALSNGAQAWTLNITGRVVDLNPYSGGTYAVVIIPAATTG
                     GTPTRNLVAISPTGTVTSTVAF"
     gene            complement(3611..4330)
                     /locus_tag="VNN25_20160"
     CDS             complement(3611..4330)
                     /locus_tag="VNN25_20160"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXH93904.1"
                     /translation="MKTLLAALAMLTATAATTPSSPVASPIPYRFDEVKRNVVLKTAT
                     RETQATKGTQAQSGDHVHTGLFSYALIAAEPHRAKFEIFSVTDVQLAGGAPGVILSLE
                     RGRVHAMFDKILGSEPRLVQTPGALLAVRGTQYNIEVDGAGKTMLDVFEGTVEIRSPF
                     RNEPYLVHAGESSSFTRHEPPPDHPTRTPDSRRPDAQNHPDGHGDDHGHPGNEPGSRG
                     AQPPPPTPQPRPPSPPPGGHH"
     gene            complement(4341..>4501)
                     /locus_tag="VNN25_20165"
     CDS             complement(4341..>4501)
                     /locus_tag="VNN25_20165"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH93905.1"
                     /translation="EDLVSLDEAEKRHIITVLRATGGNQTQAAYILGIERKTLARMMK
                     RLDISLEP"
BASE COUNT          894 a         1391 c         1519 g          697 t
ORIGIN      
        1 agttcgacac gcggcgccgc atcaaacgta gcgccgtcgg cccgacggct gttccgccgc
       61 cggctcggcg gccactttcc gcaccggcca gaaccaaagc accaacacag ccaacaccgc
      121 caccgcgcac gacccataga aactcatcgg ccgatagacc acgcgaacgt ccgaggcccc
      181 ggccggcact tcaaatccga tgaaggtgcc ttcgatatgc cgcagcggca cgcgacgtcc
      241 tccgacgtac acgcgccttc ccggcgcaag cacctcgctc gattggacca tcgttggcga
      301 cggcgcaaca accgtcatcg caaactcggc cggtgcgccg ctacgcagat tgcgcacctc
      361 ggcgccctgc gcgaagaaac gcggaagcac ggaagggttc tcccaaagcg tcccgtccgc
      421 gccgcgatag agcaggcgcc acgcacctcc cggtaatgaa tccggttcac cgaagagaaa
      481 ccgaacattc atgaagttga gctcgggccg gttcgcgtct ttgatcagcg gaagccagcc
      541 aaggtccggc tcggtgaagc ggcgcaggta gctgtcgtac gacgcgaacg ccatcggatc
      601 gctgccgcga acgtcctcca gaccgtactg cgcggaggaa tccggcagga gaacccaggc
      661 cagaccagcc acacggaacg gctcgcgcgg cgcgtgcgca cgcaacgcat cgatgatcgg
      721 cagcgggggc cggaagtact tcgcgtcgac cagcgcgttg aagccgctat tgaacgcgca
      781 aagctccgtg aagacgagga ccacggcggc ccatgagcgg aaccgttgcg gcaggaagag
      841 aaagatgaga atggcagcga ctccgatgag atcgaccggc cgcatcaggt cggagcgttt
      901 ggtgtagacg taaatcgcca gcaagaagat cggcgccgac gcgatcgcca tcgcggtctt
      961 cgacacatcc agcgccttcg ccgccacgat cgcgatcaga aagatgcaga cgaatcgcag
     1021 cttgtcgttg gcgacgatgt tgaacggcgg aatcgcattc agcgcgtgtc cgacgattgt
     1081 ccaatccatc gccacgaaaa aaagaacgat tgcgagcgcg atacgccagc gctcgcgcgc
     1141 ctccgttcgc cgggcgatca ctaccgcaag cgcgagagcc agcggaagaa ggcccgcata
     1201 cgagatggca acgccgacgt agtgctgcat ccacgaccag ttgtgccgca ccggattgcc
     1261 gaatccgttc gggctgatga tggcccacgc cgcggtgaac ggctgcatcg ggacgggggc
     1321 caccttgcgc agcgtgtcga agcgctcgct gatgcgcacc tgttcaagca cgggtaccca
     1381 cgccggcgcg ccgaccgcga ggccggcggc tacgccgagg aggggggcaa ggaggcggcg
     1441 gagaaagtcg cggactccaa aatctgaacg gacggccagc gcctcgatca agagaatccc
     1501 gccgcaaccg acagcgatgt gcagaacgct ttcgggatgg ccgttcgcca gcagcgtggc
     1561 gatgacgagc gctacgagga cgatgctggc tttcgtctgc cggtcgatcg cgtgcagcag
     1621 cgcgaaggct gccgcaggca gaaaggcgac gaccgaggcg gtcgagtaat agaggaagac
     1681 agtcattacg gtcgagaagc cgaagacgat ggcgccaaac actgccgcgg cgtcgctcgc
     1741 tttctcccgt ttgaggaaca gataggtgaa gaggagcgcg gcgaagatct tcatcgcggc
     1801 catggccacg atctgcttcg gcagcggcac gatcagcgtc gccagaaata gcggcgagaa
     1861 gggcgcggac tcgccgtttg ccagaagcgg atagccggcg aatgagtagc ggttccagag
     1921 cggcgcgcgg ccgtgggcca attcttcgcg cgcgacctgc atccacggca ggaacaactt
     1981 caccgtgtca ttggtcagcg gattggcggc aacgacgtcg ccgaacagac cgcgccacgg
     2041 atagccgcgc gcgacctcat cgaccggcac cggaagcctg ctgctgaaca cggcgccgtt
     2101 gagaaacacg agggtgagca tcatcaacag cagggcgatc gtccaaggca ccggcgcgat
     2161 ccatcgccgc acgctagcga cgaggaggac gaccgggagg acgatgccga ggagcaggag
     2221 cacaatcacg cgaagagatc gtattccagt cgccgaaaca ctcgggcggg acgcccgagt
     2281 gccgcggacg aagacgcccg cactccatcg aaccggcaac ccattgaagt aagtggccgg
     2341 ccgacgtcaa cgtcgtggtc cgcgaccgcg tgaaagaggt caatctactc ctttaaaggc
     2401 gtccgcaacc tctttaaagg ggccagtcga cctctttaaa gcggtccgca acgtcacgaa
     2461 aagggtcatt cgaccgcatc aaagccgtcc gcaactgcgt gaaaggcgtc agtcgaccgt
     2521 aatgaagccg agacggcacc tctctcgcct ctcgccgcct cctcgctcag cgcgacatcg
     2581 tggaagcagg cgccgcagag gagtcgggcg ccgacgcgaa cggcagcgcc gatgtgaaaa
     2641 cagagctcgc agcggaggcg cgaaggacgc ttcgatccgg cgacgagcgg aagtggatac
     2701 gagggtgggc gcggcatgat tggctccttt gattccggac caacgggcgg cggccacgcg
     2761 ccgccgccct cggtcgagga gggtaaaacg atcagaaagc gacggtcgac gtgaccgtgc
     2821 ctgttggact gatcgccacg agattccggg taggagtccc acccgtggtc gcagccggaa
     2881 tgatgacgac ggcgtacgtg ccgccgctgt acggattgag atcgacgacg cggccggtga
     2941 tgttgagcgt ccaggcctgc gcgccgttcg agagtgccag cgccgtgagt gtggtcgtcg
     3001 gcgacgatgc accggacgcg gtcgtgtcgg tcacttcgat gacctgcgat ccggagatct
     3061 ggacgtcggc gtgcggcgac ggcagcgtcg cgctccaggc gattgcgccg ccgcggatgg
     3121 cgatgacttc ggccaccggg gtggatgtcg tgctgctggc cgccgcgcgg acaacgacga
     3181 ccgtgccgtc ggcggcgatg ctcagatgtc cgtcaccttc gacaccatgt cccggtccac
     3241 cgggtccacc aggaccagcc ggcggagcgc cgtggtcatt cgcgacggca agcgtcgccg
     3301 ccatcatcag tgcaagaact acggtgattg ttttcttcat gggtacgtct cctttgggcg
     3361 ggagacaagt ccaagcgaaa tgccacgtcc acacagggta gaagcggcat ttggagggtc
     3421 agaacgaccc aggattgggg gtgggaggac gcaaacgagc ggcagaaaaa cccagttttc
     3481 gcgcgaagcg ctttggactg cggtagcgcc agctaccgct ttaaagcttg ggatgggcac
     3541 ccggcacgcc attcaaagcg gtggctgcgc caccgcactc caaagcgctt cgcgcgaaat
     3601 tctgcgacga tcaatgatgg ccgcccggtg gtggcgaggg cgggcggggc tgcggcgttg
     3661 gcggtggtgg ttgcgcgccg cgggagcccg gctcgttgcc tggatgtccg tgatcgtcgc
     3721 cgtggccgtc agggtggttt tgggcgtctg gacggcggct gtcgggggtt cgggtcggat
     3781 gatcgggcgg cggctcgtgg cgcgtgaagc tcgagctctc gccggcgtgg accaggtagg
     3841 gctcgttgcg aaacggtgag cggatctcga cggtgccttc gaacacgtcg agcatcgtct
     3901 tgccggcgcc gtcgacttcg atgttgtact gcgtgccgcg caccgccaga agagcgccgg
     3961 gcgtctgcac gaggcgcggc tcgctgccga ggatcttgtc gaacatcgcg tggacgcggc
     4021 cgcgctcgag tgagaggatc acgccgggcg cgccgccggc gagctgaacg tcggtgacgc
     4081 tgaagatctc gaacttcgcg cggtgcggtt ccgcggcaat cagggcgtag gaaaagaggc
     4141 cggtgtgaac gtggtcgccg ctctgcgcct gcgtgccttt cgttgcctgc gtctcccgcg
     4201 tcgctgtttt cagcacgacg tttcgcttga cctcatcgaa tcgatacgga atcggcgagg
     4261 caacgggact cgacggcgtt gtcgccgctg tcgcggtgag cattgccaga gctgccagga
     4321 gtgtcttcat gagggcctca tcagggttcg agcgaaatat cgagtcgctt catcatgcgc
     4381 gcaagggtct tgcgctcgat gccaagaatg tatgcggcct gcgtctgatt cccgcccgtg
     4441 gcacggagga ctgtgatgat gtgccgcttt tcggcctcgt cgagcgagac aagatcctcg
     4501 c
//
LOCUS       DATLVW010000695         3211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_239134, whole genome shotgun sequence.
ACCESSION   DATLVW010000695 DATLVW010000000
VERSION     DATLVW010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239134"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VNN25_20170"
     CDS             complement(<1..717)
                     /locus_tag="VNN25_20170"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXH93906.1"
                     /translation="MTDILTLPVEPKSVVSNPSPDELRRYTEEMPNCRKTEFDNVNVA
                     TRVVSRSKGSTFVATDTPENHSDQTISRAEYDRMAKLQNDYIRTREMLVVDGFIGNDP
                     AFRVPARLIIEKANANIAAMQQHLYYPATPAELDSFEPVVTVIYTPNLKAEGYPEERL
                     IAVDLDHYITRVFNSDYFGESKKGGLRMWNKIVYERGGLALHAGCKVIPVDGENKVGL
                     IVGLSGTGKTTTTFTKQNNSQ"
     gene            859..1155
                     /locus_tag="VNN25_20175"
     CDS             859..1155
                     /locus_tag="VNN25_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93907.1"
                     /translation="MYLVRDVFRCKPGKAGELAARFKRTIPSMESEDRFSNCRVMVDA
                     VASYWTVVLEAEFASLADFEHHMADYSKRADVREAMAGYMDLVEGGHREIFKLV"
     gene            1158..2081
                     /locus_tag="VNN25_20180"
     CDS             1158..2081
                     /locus_tag="VNN25_20180"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXH93908.1"
                     /translation="MRIAIVGAGGVGGYFGGKLAQGGEDVVFIARGATLEALRTTGLR
                     VDSIKGDFVIEHPNATDDPATVGPVDAIFVTVKTFQVPAAAEAIHPMIGADTIVVPLE
                     NGIDASDQLANVVGREHVLGGLCGIVSFIAAPGHIRHVGAEPFVMFGELDNRTSKRAE
                     RLRDACLHAGVQADIPPDIHHALWSKFVFIAPMSGIGAATRVPVGIWRSMLETRALAA
                     SAIREIVSVAMAHGVDLGGEAAVDRTLARFDGLPPDATSSLQRDLMEGRRSELDAQLG
                     AVVRLGLAAGVATPVCETLLALLLPQELASR"
     gene            2276..>3211
                     /locus_tag="VNN25_20185"
     CDS             2276..>3211
                     /locus_tag="VNN25_20185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXH93909.1"
                     /translation="MSKPPKTAAIRDSNLHPALAEARRVLEVESAAILGLIDHLDQTF
                     VDVVETINATSGRVVSMGLGKSGIICKKISATLASTGTPSFFLHPAEAIHGDLGMIVR
                     GDVVLAISNSGETEELVRLLPSIKRIGAEIVAITGNPQSSLARTADFHLSAAISKEAC
                     PLGLAPTASTTATLALGDALAMALLLRKGFKEEDFAFLHPGGKLGKRFLRVSDLMHAG
                     KQVPSVLLDTPMHDVIYEMSDKGFGITAVVDDQGVLAGVISDGDLRRLLQRDTDVLKK
                     TAADCMKPDPVTIEGKELASAALEMMEQRKITSIFI"
BASE COUNT          594 a         1023 c          972 g          622 t
ORIGIN      
        1 ctgcgaattg ttctgcttcg tgaacgtcgt cgtcgtctta ccggtgcccg acaatccgac
       61 gatgagaccc actttgttct cgccgtcgac cggaatgact ttgcatcccg cgtgcagggc
      121 caaaccgccg cgctcgtaga cgatcttgtt ccacatccgc agtccgccct tcttcgactc
      181 cccgaagtag tcggagttga agacgcgggt gatgtagtga tcgaggtcga cggcgatgag
      241 cctctcctcc ggatatccct cggccttcag attcggtgtg tagatcaccg tcacgacagg
      301 ctcgaaactg tcgagctccg ccggcgtggc gggatagtaa aggtgctgct gcattgcggc
      361 gatgtttgca ttggccttct cgatgatcag ccgcgccggc acccgaaacg ccggatcgtt
      421 gccgatgaat ccgtcgacca cgagcatctc gcgcgtccgg atgtagtcgt tctggagctt
      481 cgccatgcgg tcgtactccg cgcggctgat cgtctgatcg gagtgattct ccggagtgtc
      541 ggtggccacg aatgtcgagc ccttcgagcg cgacaccacg cgggtggcta cattgacgtt
      601 atcgaactcg gtcttccggc agttcggcat ctcttccgtg tagcgccgaa gctcatcggg
      661 cgacggattc gacacaacag acttcggctc tacgggaagc gtcaggatat cggtcatgag
      721 tcgtcctctc gacggatcag attgtgatgc tgtttcgacg caagaactgc gccgcgaaga
      781 ccgcacgcgt ccccgcgaag gtgcgggatt ttacacgaaa ccatagaatg cgcacggccc
      841 gacgcaggag gcaaacgtat gtatctcgtt cgcgatgttt ttcgatgcaa accggggaaa
      901 gccggcgagc ttgccgccag attcaagcgg accattccgt cgatggaaag cgaggaccgc
      961 ttcagcaact gccgtgtcat ggtcgatgcc gtagccagct actggaccgt cgtgctcgaa
     1021 gccgaattcg cctctctcgc cgacttcgaa catcacatgg ccgattactc caagcgcgcc
     1081 gacgttcgcg aagcgatggc tggctacatg gatctcgtcg aaggcggcca ccgcgagatt
     1141 ttcaagttgg tctgatcatg cgaatcgcaa ttgttggcgc cggcggcgta ggcggctact
     1201 tcggaggcaa actcgctcag ggcggcgagg atgtggtttt catcgctcgc ggcgcgacac
     1261 tcgaagcctt gcgcacgacg ggactccgcg tcgatagcat caagggcgac ttcgtcatcg
     1321 aacatccgaa cgccacggac gatcccgcga cggttggtcc ggttgacgcg atcttcgtca
     1381 ccgtcaagac gtttcaggtg ccggcagcgg cggaggcgat ccatcctatg atcggcgccg
     1441 atacgatcgt tgtcccgctg gagaacggca tcgacgcgtc cgatcaactg gccaatgtgg
     1501 tcggccgcga gcacgtgctc ggcggcttgt gcggcatcgt cagcttcatc gccgcgccgg
     1561 gccacatacg ccacgttggt gcggagcctt tcgtgatgtt cggcgagctt gataaccgga
     1621 ccagcaagcg tgcggaacgt ctccgcgacg cctgcctcca cgccggtgtg caggcagaca
     1681 tcccgccgga cattcatcac gcgctctggt cgaagttcgt cttcatcgcg ccgatgagtg
     1741 gcatcggtgc cgcgaccaga gtgccggtcg gcatctggcg ttcgatgcta gagacgcgcg
     1801 cgctcgccgc cagcgccatc cgcgagatcg tcagtgtcgc catggcccac ggcgtcgacc
     1861 tcggcggcga agccgcggtc gatcggacgc tggctcgttt cgacggcctc cctccggacg
     1921 ctacctcgtc cctgcaacga gacttgatgg aaggccgccg ctccgagctc gatgcccaac
     1981 tcggcgccgt cgtccgcctc ggactcgcgg cgggagtagc gacgcccgtg tgcgaaacat
     2041 tgctcgcgtt gttactcccg caagaactcg catcacgata ggagtgcggt cgtctcgacc
     2101 gcggccggaa ggcgtctcgc ctgacggtgg cattacgcaa agcgtcgcat ttgccagtgt
     2161 gtgtccctaa cgcaagacat cgcatccttg cgaaagcccc gcgacacacc gaggcgagac
     2221 gcctcgctgc cgcggtcgag acgaccgcac tccttttcgc ataccatctc gggggatgtc
     2281 caaacctccg aagactgcgg caattcgcga ttccaacctt caccctgccc tcgccgaggc
     2341 gcggcgcgtg ctggaagtcg aatcggcggc aatcctcggg ctgatcgacc atctcgatca
     2401 gaccttcgtc gatgtcgtcg aaaccatcaa cgccaccagc ggccgggtcg tgtcgatggg
     2461 actcggcaag tcggggatca tctgcaagaa gatcagcgcc acgctggcgt cgacgggaac
     2521 gccctcgttc ttccttcatc ctgccgaggc cattcatggc gacctcggga tgatcgtgag
     2581 aggggacgtc gtcctggcga tctccaactc tggcgaaacc gaggagctgg tacggctcct
     2641 tccgagcatc aagcgcatcg gtgcggagat cgtggccatc accggaaacc cgcagtcatc
     2701 gctggcgcgc accgccgact tccacctctc cgcggccata tcgaaagagg catgtcccct
     2761 cggcctcgcg ccgacagcgt cgaccaccgc aacgctcgct ctcggcgacg cattggccat
     2821 ggccttgctg ctgcgcaagg ggttcaagga agaggacttc gcattcctcc atcccggcgg
     2881 caaactcggc aagcgtttcc tgcgcgtcag cgatctgatg cacgcaggca aacaggtgcc
     2941 ctccgtcctg ctggacacgc cgatgcacga cgtcatctac gagatgtccg acaaaggctt
     3001 cggcattacc gcggtcgtcg acgatcaggg agtcctcgcc ggagtgatct cggacggaga
     3061 tcttcgccgc cttttgcagc gcgacaccga tgtactgaaa aagaccgccg ccgactgcat
     3121 gaagcccgat cctgtgacga tcgaagggaa ggagctggcg tccgcggcgc tggagatgat
     3181 ggaacaacgg aagattacgt cgatcttcat t
//
LOCUS       DATLVW010000696        12222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_607952, whole genome shotgun sequence.
ACCESSION   DATLVW010000696 DATLVW010000000
VERSION     DATLVW010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607952"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..836)
                     /locus_tag="VNN25_20190"
     CDS             complement(150..836)
                     /locus_tag="VNN25_20190"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXH93910.1"
                     /translation="MRRSVRFLFVLAVVVGMGPSVAQPIPPPLQKPFTVRSLWPQLKA
                     GNDLFASGVLRYTELNGIRRRTASGQNPPITVLACSDSRVPPELVFGRGLDDLFVVRT
                     AGNVADTFGIASIEYGVAHHWSKVIIVLAHDRCGAVTEALKTVEPDTPSLIELVHRIR
                     DGIKGADLGTKKRDMVRPAVIANAKASAHYLVDHSEVIKNAVDKKEVEIVVAYYGFSD
                     GKVVRLETIR"
     gene            complement(870..1484)
                     /locus_tag="VNN25_20195"
     CDS             complement(870..1484)
                     /locus_tag="VNN25_20195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH93911.1"
                     /translation="MVAIVTGASSGLGLEIAQQLRARGTLVVGVSRRASADVAGDASK
                     RETALRAIEAAERLGTIDLLVNCAGLGIFGPTGSYDESSITAVINSNLIATINFCELL
                     FPRFKEHGGTIVNTLSTAALIGKPNESVYCAAKWGARGYGESLKAEAKGTKARIIAVA
                     PGGMNSPFWSEPRNGFMDPADVAATIIDAINRPVDISEIVITRR"
     gene            1543..2877
                     /locus_tag="VNN25_20200"
     CDS             1543..2877
                     /locus_tag="VNN25_20200"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HXH93912.1"
                     /translation="MRNDRRAVVWTCLAIWLFAVGYAVITPPFQVPDEVAHYWHAVSL
                     AHGNVFHVMQNDRMGTFVPRSARDFVYLTWVETAGKPENKIGMARLRAANELPYQPEP
                     VFRSFPALYTPVPYLPGTLASAVADVLHVRPLIAFYAGRIATATACVLLIAAAMLECV
                     ELAAIIAAIALLPMTLYMFGSFSADAVTISLALYTTAVAWRVRSGDTMNARRWVRLCL
                     AAAALALCKMLYAPLALLALAPRASGHTKAPNRLRQVISLFVSLAIGLALSAAMVSRQ
                     YHPLRPEANPKVQLQTIAAHPIHVASVVAKDFVAQFAGYRDQFVGRLGWVDVPLSSFL
                     TVAVFYLLLAVAMTRSFAITPAARLFALLLTCLLFFAVSLSQFIAWTPPGGEYVDGIQ
                     GRYFLPLAVLILLPIVSIVRNKRVGFAALGVYALTAVAINIDAFVVLVKRYY"
     gene            complement(2947..4887)
                     /locus_tag="VNN25_20205"
     CDS             complement(2947..4887)
                     /locus_tag="VNN25_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93913.1"
                     /translation="MKFPRHAAAAVVFVVLAVFMTWPLLPNLNRAVAYPGDPYINTWI
                     LDWDYHATFHQPVRLFQANIFYPAKYSLAFSENLYGIAIILFPLRIVGVSPLTAYNVA
                     MLLGFAMCGFFAYLLAREVTGSTVGAIAAGIFYAFVPFRFTHSSHVQYVWAGTLPLLL
                     YALIRYARVPDWRHAIGFGAAFLWNGLSNVHFFFFGSIAIVMTIALLRPPLRRIAVTL
                     TVALLLLAPFLYPYHEASKLYGMERSWDETLSYSAQPADWLVDNFAHRPYPTHSDPTV
                     NPERWLFPGALALCLALIAIASRQWQSLSLGWSWVLLGFIGSLGLHTPFYRLLFKYVP
                     GFHAIRAPARWAVITHVGLALLVAVAVGWIAKRAVWVGVVASIAFIAELHGPIRWYMA
                     PTVERPVDRWVATARPHAIAELPMSDQNDEYGVLLNATVHHRSIVNGVSGFAPPEYVK
                     LASLAQSWSDDLVPELARIGVTHVIVRGVGLDTAGRAWLGHMVDRGLLGVVRRFDDGA
                     WGDWVFRVGVRGDSPELRSMLGGHVTYNESTFGALVQPSGHEPLKGRVTFSGVALSPF
                     GVREVNLLFDNGRARFPATLVADPATSRWYRFYDATTKPSFARTFEARPANVRLLTDV
                     QVEIVDGRGERTLLDDRWIDWN"
     gene            complement(4919..5872)
                     /locus_tag="VNN25_20210"
     CDS             complement(4919..5872)
                     /locus_tag="VNN25_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HXH93914.1"
                     /translation="MRKLLIASALVLFAVASIAADVKTGGAKMIEVDGKYHVWTDRVG
                     HGPVKMLTLHGGPGFPHDYFECFEDFLPQHGIQFFYYDQLGVGNSDVPDDVSLWTIPR
                     YRSEVEQVRKGLGLDHFILYGHSWGGMLAIEYALEHQDHLKALIISDMTAGIPAYEKY
                     AATLLAELSPEDQKTLAKFAAAGNYDAPEYQEIMMGKVYAQHLIRLDPWPEPVARAFR
                     KFNQKIYNEMQGPNEFVISGNFKSWDRWADLPKIKVPTLIIAGRHGTMSPDDIRRMGK
                     LIPHSRVVITEGSHLEMYDDQDRYFREVVKFIKDVEAGKMK"
     gene            6008..7510
                     /locus_tag="VNN25_20215"
     CDS             6008..7510
                     /locus_tag="VNN25_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HXH93915.1"
                     /translation="MTDAVIQHEDGRVELRSDEGIRTSALYNHDLAPVPVSQRNWTTY
                     NYAALWIAMEHCIPTYMMSSGLIGAGMNWWQALLTILVGNTIVLIPILLNSHAGTKYG
                     IPFPVFARASYGTIGSNLPALMRAIVACGWFGIQAWIGGEALHTFFTSILPAWPSLLG
                     KFAGHPTTEWISFLLFWALNILIIYRGMDLLRRFENWAAPFVLVMTALLLGWAVWRAH
                     GLGYLLHEKGKFETWAQFRPIFIPSLTAMIGFWATLSLNMPDFTRFGRSQREQAIGQT
                     VALPTTMTVFAAMGVIITSAAVVIYPTMGINELWDPLKLVAQFKQPLVVAISMFTVVI
                     ATLSVNIAANVVSPANDFANAFPKWISFRTGGLITGLLAIAMQPWRLLADPSGYIFTW
                     LLGYSGGLGSIGGVMIVDYWIVRKKHLVLGDLYRTEGTYRYAAGWNWRAVIATLLGCT
                     AAWIGLVVPALRPVYDYAWFVGFGVAALTHLILMKVMPPRDIVEATPAPA"
     gene            8905..9774
                     /locus_tag="VNN25_20220"
     CDS             8905..9774
                     /locus_tag="VNN25_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXH93916.1"
                     /translation="MSRIVKGGLIQTSLACDTSESLDRIREAQTERTLKYIDQAGAEG
                     VQILCMQEIFTGPYFCAEQSTRWYDSVEKIPDGPTTKLMQEYAKKYNMVIVVPLYEEE
                     ETGVYYNTAAVIDADGKYLGKYRKNHIPHVAPGFWEKFYFRPGNLGYPVFETAYAKVG
                     VYICYDRHFPEGARELGLAGAEIVMNPSATVAGLSEYLWKLEQPAHAVANGYFIGAIN
                     RVGYEAPWNIGEFYGQSYFCDPRGQMLAVGPRDADALVTADLDMDKIREVRNTWQFYR
                     DRRPETYSGIVRL"
     gene            9871..11265
                     /locus_tag="VNN25_20225"
     CDS             9871..11265
                     /locus_tag="VNN25_20225"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXH93917.1"
                     /translation="MRDHILIHINGKPHRIDGDAAFETLANYLRQDVAATGTKIVCEE
                     GDCGACTVLTRRSANDDYRAVNSCILNLAQLDGSSIVTVEGLREEKSLHPVQEAMVQC
                     HGAQCGYCTPGFVVAMAGMFETCDRVNEKQVRDGLTGNLCRCTGYEPIIKAALAVDGA
                     PAAKMRDRYPSLPDANDSVQVGTFCAPATLDEAIAFKREHPGCTIVQGGTDVGVWVNK
                     RNYAAPAMLSLAKIDSLSELHEDDGTIVVGANTTLADFETFIADRIPELAGIMAIFGS
                     PQIKNAGTLVGNIANGSPIGDTLPYLFVAGAMLEMNGGTRSVPIDRFYLGYRKFDLQP
                     DEIITRVRIGIVRDKLKLYKVSRRKDLDISAFTAAIRLAMTGDHIDRAGVAYGGVAPT
                     VVRLPRTEAFLTGKTATLDNFEQAGRIARAEIKPISDVRGSADYRLQLAENIMAKFWF
                     EVFGQTSAPRLAHA"
     gene            complement(11298..11603)
                     /locus_tag="VNN25_20230"
     CDS             complement(11298..11603)
                     /locus_tag="VNN25_20230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HXH93918.1"
                     /translation="MSRSSTQSGAQSSSRSTSSDPCAFCGNAEVSLRYVTRSYGSGAT
                     LLVIENIPMFSCPRCGESYFTAETLHEVSRIKAKRRSMAALRPVPVAVFPKRPIAAA"
     gene            complement(11545..11826)
                     /locus_tag="VNN25_20235"
     CDS             complement(11545..11826)
                     /locus_tag="VNN25_20235"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HXH93919.1"
                     /translation="MADRTLPRLRDLVRQHRYLVSSHAADELENDELDILDLESIVLT
                     GEIIERQRDRKTGEAKYVIRGITLANGIGSVVAKFDSVGRAVVITVYLE"
BASE COUNT         2354 a         3989 c         3635 g         2244 t
ORIGIN      
        1 cctcgttctt gaccgcgttg cgaatgattt cgcgaacctc ttcatccaag ctgcggccgt
       61 gtgctggacg cacgctgccg aagcttttcg atcacatcct cgagctcaat cacgagctga
      121 tccgcgggtt cggcgatctc cctcatcgct caccgaatcg tctcgagcct cacgaccttc
      181 ccgtccgaaa acccgtagta cgcgacgacg atctccactt ctttcttgtc cacggcgttc
      241 ttgatcacct cgctgtggtc aacgaggtaa tgcgctgagg ccttggcgtt cgcgatcacc
      301 gcggggcgga ccatgtcgcg cttcttcgtg ccgaggtcgg cgcctttgat cccatcacgg
      361 atgcggtgca cgagctcgat cagactgggt gtgtccggtt ccaccgtctt cagcgcctct
      421 gtgactgccc cgcatcggtc atgagccagg acgatgatga ctttcgacca gtgatgcgcg
      481 acgccgtatt cgatgctggc gattccgaag gtgtcggcga cattgccggc agtgcgaacg
      541 acgaaaagat cgtcgaggcc acgtccgaag accagttcgg gcggcacgcg gctgtccgag
      601 cacgcaagca cggtgatcgg cggattctgt ccgctcgccg tcctgcggcg tatgccattg
      661 agctcggtgt aacgaagcac gccactcgcg aacaagtcgt tgcccgcctt gagctgcggc
      721 cacagcgatc tgacggtaaa gggcttctgg agtggtggcg ggattggctg tgcaaccgac
      781 ggccccatac ccacgacgac agcaagcaca aagaggaaac ggacggatcg gcgcatggac
      841 tgacctccgc cgcgaatctt aacgcccaat catcgccgcg tgatgacgat ttcgctgatg
      901 tcgaccggac gattgatggc atcgatgatc gtggctgcga catccgcggg gtccatgaag
      961 ccattgcgcg gctcactcca gaatggcgaa ttcatcccgc caggcgccac cgcgatgatc
     1021 cgcgccttcg ttcctttcgc ctcggctttc agcgattcgc cgtaaccgcg cgcgccccac
     1081 tttgcggcgc agtagacgct ctcgtttggt ttgccgatga gagccgccgt cgaaagagtg
     1141 ttgacgatcg taccgccgtg ctctttgaaa cgcggaaaga gcaactcgca gaagttaata
     1201 gtggcgatga ggttcgagtt gatgaccgcg gtgatggagg attcgtcata cgagccggtc
     1261 ggaccaaaga tgccgaggcc tgcgcagttg acaaggagat cgatggtgcc gaggcgctcg
     1321 gctgcttcta tggcgcgcaa ggcagtctcg cgcttcgagg catcgccggc cacgtcagca
     1381 ctcgctcgcc gcgaaacgcc gacgaccagc gtgccgcgtg cgcgcaactg ctgtgcgatt
     1441 tcgagtccga gcccgctcga ggctccggtg acgatggcga ccacgcctcg attgtaaggc
     1501 ttgctctacg tgagcaactg tacgccgggt aagatgggct caatgaggaa cgatcggcgg
     1561 gccgtcgtct ggacttgtct ggcgatctgg ctcttcgcag tcggttacgc ggtcatcacg
     1621 ccgccctttc aggtgccgga cgaggtcgcg cactactggc acgccgtttc gctcgctcac
     1681 ggcaacgtct ttcacgtgat gcagaacgat cggatgggca cgttcgtccc gaggagcgca
     1741 cgcgatttcg tttacctgac gtgggtcgag accgccggca agcctgagaa caaaatcgga
     1801 atggcgcggt tgcgcgcagc caacgagttg ccgtatcaac ccgagccggt cttccgctcg
     1861 tttcccgcgc tctatacgcc ggttccgtac ctgcccggca ctctcgcgag tgcggtagcc
     1921 gacgtactgc atgtgagacc gctcatcgcg ttttatgccg gccggatcgc aacggcaacg
     1981 gcctgcgtac tactgatcgc cgcggcaatg cttgaatgcg tcgagcttgc cgcgatcatc
     2041 gccgcaatcg cgttgctgcc gatgacgctc tacatgttcg ggtcgttctc cgcagacgcc
     2101 gtaacaatca gccttgcgct ttatacgacc gccgtagcct ggcgcgtacg gtcgggtgac
     2161 acgatgaatg cgcggcggtg ggtgaggctc tgcctagctg ccgcagcatt ggcgttgtgc
     2221 aagatgctgt acgcgccgct cgcgttgctg gcgctcgcgc cacgcgctag cggtcacacg
     2281 aaggctccaa acaggctacg gcaggtgatc tcgctctttg tgtccctcgc gatcgggttg
     2341 gcgttgtcgg cggcaatggt gtcgcggcaa taccatcctc tccggccgga agcgaatccg
     2401 aaggtgcaat tgcagacaat cgctgcgcac cccatccacg tcgcgagtgt cgttgcgaaa
     2461 gacttcgtcg ctcagttcgc cggttaccgc gaccagttcg tcggccgcct cggatgggtc
     2521 gatgtcccgt tgtccagttt cctgactgtg gccgtgttct atctgcttct tgctgtggcg
     2581 atgacgaggt cctttgcgat cacgccggcc gcgcggctct tcgcgctgct actcacgtgc
     2641 ttgcttttct tcgcggtgag tctctcgcaa ttcatcgcct ggacgccacc tggcggggag
     2701 tacgtcgatg gcattcaggg acgctacttt ctgccattgg cggtactcat tttgctgccg
     2761 attgtgtcca ttgtgcgcaa taagagagtt ggttttgcgg ctctcggtgt ttatgcgctg
     2821 accgcggtgg cgatcaatat cgatgcgttt gttgtgctgg tgaaacgtta ttactaaagg
     2881 cacttaccac agagacacag aggacacaga gaaatgctct gtgctctctg tgtctctgtg
     2941 gttgaatcaa ttccagtcaa tccagcgatc atcgagcagg gtgcgctcgc cgcggccgtc
     3001 gacgatctcc acctgcacgt cagtcaggag ccgcacattt gccggcctcg cctcgaacgt
     3061 tcgcgcaaat gacggtttcg tagtcgcgtc ataaaagcga taccagcggc tggtggcggg
     3121 atcagcaacg agcgtcgccg ggaaccgggc cctcccgttg tcgaagagaa gattcacctc
     3181 gcgcactccg aacggcgaga gcgcgacgcc ggaaaacgtc acccgtcctt tgagcggctc
     3241 gtggccggag ggctgaacga gggcgccgaa cgtcgactcg ttgtacgtca cgtggccgcc
     3301 cagcatcgac cgcagctccg gcgagtcgcc acgcaccccg acacgaaaga cccagtcgcc
     3361 ccatgcgcca tcgtcgaaac gccgcacgac gccgaggaga ccgcggtcga ccatatggcc
     3421 cagccaggcg cgtccagccg tatcgaggcc aacccctcgc acgatcacgt gcgtgacgcc
     3481 gatgcgcgcc agctccggta ccagatcatc cgaccacgat tgcgcgagcg aggcgagctt
     3541 cacgtactcg ggcggcgcga agccggagac gccgttgacg atcgaccggt gatgcacggt
     3601 ggcgttgagc aggactccgt actcgtcgtt ctgatcggac atcggcaatt cggcaatggc
     3661 gtgcggacga gccgtcgcca cccagcgatc gacgggacgc tcgacggtcg gcgccatata
     3721 ccagcgaatc gggccgtgca gttcggcgat gaatgcaatc gatgcaacga cgccgaccca
     3781 caccgcacgt tttgcgatcc acccgacagc gaccgcgacg agaagcgcaa gcccgacgtg
     3841 tgtgatcacc gcccagcgcg ccggcgcacg aatggcgtga aatcccggca cgtacttgaa
     3901 gagcaacctg tagaagggcg tgtgcagtcc gagggagccg ataaatccga gaaggaccca
     3961 gctccatccc agagacagtg attgccattg tcgcgaggcg atcgcgatga gtgcaaggca
     4021 aagcgccagc gcacccggaa agagccatcg ttccgggttg acggtcgggt cactatgcgt
     4081 cggatacgga cgatgcgcaa agttgtcgac cagccaatct gccggctgcg cggagtaact
     4141 caatgtctca tcccatgagc gctccatccc ataaagcttc gacgcctcgt gatacgggta
     4201 gagaaacggc gcgagcagca atagagccac agtcagcgtg acggcaatgc gccggagcgg
     4261 cggccgcaat aacgcgatcg tcatcacaat ggcgatgctg ccgaaaaaga agaagtgaac
     4321 gttcgacagc ccgttccaca agaaggcggc cccgaagccg atggcgtggc gccagtcggg
     4381 cacccgcgcg taacggatca gcgcatagag cagcagcggc aacgttcccg cccagacata
     4441 ttgcacgtgc gacgagtgcg tgaagcgaaa aggaacgaat gcatagaaga tccccgccgc
     4501 aattgctcca acggtgcttc cggtgacttc tctcgcaagg aggtaagcaa agaatccgca
     4561 catcgcgaac cccagcaaca tggccacgtt gtacgcggtc agcggcgata cgccgacgat
     4621 gcgtagcgga aaaagaatga tcgcgatgcc gtagaggttt tcgctgaagg caagcgagta
     4681 cttcgcggga tagaagatgt tcgcctggaa caaccgcacg ggctgatgaa acgtggcgtg
     4741 atagtcccag tcgaggatcc atgtgttgat gtagggatcg cccggatagg cgacggcccg
     4801 attgagattc ggcagcagcg gccacgtcat gaagacggcg aggaccacga agacaaccgc
     4861 cgcagcggcg tgcctgggaa acttcacgaa gcgatgatag cggcagccga agccgccctc
     4921 acttcatctt ccccgcctcc acatccttga tgaacttcac cacctcgcgg aaataccgat
     4981 cctggtcgtc gtacatctcc aggtggctcc cctcggtgat cacgacgcgc gagtgcggga
     5041 tgagcttgcc catgcggcgg atgtcgtccg ggctcatcgt gccgtgacgg ccggcgatga
     5101 tcagcgtcgg aaccttgatc ttcggcagat cagcccatcg atcccaactc ttgaagttgc
     5161 cgctgatgac gaactcgttc ggcccctgca tctcgttgta gatcttctga ttgaacttcc
     5221 ggaacgcgcg cgctaccggc tcgggccagg gatcaaggcg gatcaggtgc tgagcgtaca
     5281 ccttccccat catgatttcc tggtactcgg gcgcgtcgta gttccccgcg gcggcgaact
     5341 tcgccagtgt cttttgatcc tccggcgaca gctcggccaa tagcgtcgcc gcgtatttct
     5401 cgtacgccgg gatgccggcg gtcatgtctg aaatgatcag cgctttcagg tgatcctgat
     5461 gctcgagcgc gtactcgatg gccagcatgc cgccccatga gtgaccgtag aggatgaagt
     5521 gatcgagccc gaggcctttg cgaacctgct cgacttcaga gcggtagcgc ggaatcgtcc
     5581 acagcgagac atcgtcgggc acatcggagt ttccgacgcc aagctggtcg tagtagaaaa
     5641 actggatccc gtgctgcggc aggaaatcct cgaagcactc gaagtagtcg tgcggaaagc
     5701 ccggcccgcc gtgcagcgtc agcatcttca ccggaccatg cccgacgcgg tcggtccaga
     5761 cgtggtattt cccgtcgacc tcgatcatct tcgcgcctcc cgttttcaca tcagcggcga
     5821 tggacgcgac ggcaaacaga acgagagcgg atgcaatcaa gagctttcgc atgacaaatg
     5881 atacgcaccg aacccgcgtc cggatgcctc gccgcgtcta ccactcgccg caccccactc
     5941 cccacaaccc cgtatggcag aatgtcgccc ttcccctcac tttgggaaac cgcacgggag
     6001 gcgatcgatg actgacgcag tcattcaaca cgaagacgga cgtgtcgagc ttcgctcaga
     6061 cgaaggcatt cgcaccagcg cgctctacaa ccacgatctc gcgcccgtgc cggtctcgca
     6121 gcggaactgg actacgtaca actacgccgc gctctggatc gccatggagc actgcatccc
     6181 gacctacatg atgtcgtcgg gcctcatcgg cgcggggatg aactggtggc aggcgctgct
     6241 cacgatcctg gtcggcaaca cgatcgtgct catcccgatc ctgctcaact cgcacgccgg
     6301 caccaagtac ggcatccctt tccccgtctt cgcgcgcgcc agctacggca cgatcggctc
     6361 gaaccttccg gcgctcatgc gcgccatcgt cgcctgcggc tggttcggaa ttcaggcatg
     6421 gatcggcggc gaggctctgc acacgttctt cacctcgatc cttcccgcgt ggccatcgct
     6481 gctcggcaag ttcgcgggac atcccaccac ggagtggatc tcgtttcttc tcttctgggc
     6541 gctgaacatc ctgatcatct accgcggcat ggacctgctg cgccgcttcg agaactgggc
     6601 tgcgccattc gttctcgtga tgaccgcgct cctcctcggc tgggccgtct ggcgcgcgca
     6661 cggactcggc tatctgctac acgagaaagg caaattcgag acgtgggcgc agttccggcc
     6721 gatcttcatc ccatcgctca ccgcgatgat cggcttctgg gccacattgt cgctgaacat
     6781 gcccgacttc acccgtttcg gacgcagcca gcgcgagcag gccatcggcc agaccgtcgc
     6841 cctgccgacg acgatgaccg tcttcgcggc gatgggcgtc atcatcacct ccgcggcggt
     6901 cgtcatctat ccgacgatgg gtatcaacga gctttgggat ccgctcaagc tcgtcgcgca
     6961 gttcaaacag ccgctcgtgg tcgcgatctc gatgttcacc gtcgtcatcg cgacgctgtc
     7021 agtcaacatc gccgcgaacg tcgtctcccc tgcgaatgac ttcgccaacg ccttcccgaa
     7081 gtggatctcc ttccgcaccg gcggcctgat caccggtctg ctcgcgattg ccatgcagcc
     7141 gtggcgtctt ctcgctgatc cctccggcta catcttcacc tggctcctcg gctactccgg
     7201 cggcctcgga tcgatcggcg gcgtgatgat cgtcgactac tggatcgtgc gaaagaagca
     7261 tctcgtcctc ggcgatctct atcgcaccga aggcacatac cgctacgccg ccggctggaa
     7321 ctggcgcgcc gtcatcgcca ccctcctcgg ctgcaccgca gcatggatcg gcctcgtcgt
     7381 ccccgccctg cgccctgtct acgactacgc ctggttcgtc ggcttcggtg tcgccgcgtt
     7441 gacgcacctc atcctgatga aggtcatgcc gccgcgcgat atcgtggaag caactcccgc
     7501 ccccgcatga tcacactact catcctcggc ggcgccgcag tagccgcaat cgtggccggc
     7561 gccaccctca agcgaaaaaa aggagtgcag gcgtcttcgc ctgcggccgc cgggcgtccc
     7621 cgcccggcgg tggttacgaa tccgaacttc gcaccgcagg cattggcgat gcccgatcct
     7681 gagccatacc ccaccaccat ccccgatttg ccgccagttt ccgataccga gccgctaccc
     7741 gacgccgacc cggagccact cccaatcatc gagcctcaac caacgcccga accgctgcaa
     7801 tcagcggagc gccacgtaga gcaggcgtcc ttgcctgctc ccgccgggcg tccctgcccg
     7861 gcggtggttg cgagtccgga ccagacgaca gaaacgtccg agctggcaca agtcccatca
     7921 gtagcgatcg aaatgggagc gaatcatgcg cttgaacttc cttccacacc gatcgaccca
     7981 gcgactgcgg aacaggctcc ttatcccgcg tttcgcacca acacaccgcc gggcagggac
     8041 gcccggcggg agcaggcagg gacgcctgct ctacgatcgg acgtcgcacc atgggcattg
     8101 gcgatgtccg atcctgagcc gcaaccgccc acggtccctg atttgccgcc cgtcaccgat
     8161 cccgagcctc aacccgacac cgacccggag ccactcccga tcatcgagcc tcaacgagcg
     8221 cccgaaccgc tgcaaacagc agagcgcgac gtgaagcagg cgtccttgcc tgctcccgac
     8281 gggcgtcccc gcccggcggt ggttgcgaat tcggaccaga cgcgagaaac gtccgagctg
     8341 gcacaagtcc catcggtagc gatcgaaatg ggagggaatc atgcgggagt cgtgtcgcaa
     8401 gcgcttgaac ttccttccgc gccgatcgaa ccagggacgg cggctcctaa ccccgcgttt
     8461 cgcaccaaca caccgccggg cagggacgcc cggcgggagc aggcagggac gcctgctcta
     8521 cgatcggacg tcgcaccacg ggcattggcg atgtccgatc ctgagccgca accgcccacg
     8581 gtccctgatt tgccgcccgt caccgatccc gagccgctcc ccgacgccga ccccgagcca
     8641 cttccgatca tcgagcctga gcctcagcca gcccctgagc cgccgccacc gcccgaacca
     8701 aaacaaccca cgatcatcgt cgcccccgag cgatcgccca aacagataat cgctcccaca
     8761 ggtcgcctaa gccggaggca atcagcgccg tcggccgtgc cgacccggag gcaactaccg
     8821 ccgtcggccg tgccgacccg gaggcaacca acgccgtcgc ccgtgccgac tataatgcgc
     8881 ggaccaaaat cggagaaaac gaacatgtcc cgaatcgtca aaggtggttt gattcagacg
     8941 tctcttgcct gcgataccag cgaatcgctc gacagaattc gcgaggccca gaccgagcgc
     9001 acactcaagt acatcgacca ggccggagcc gaaggcgtgc agatcctctg catgcaggag
     9061 atctttaccg gtccctactt ctgcgccgaa cagagcacgc gttggtacga ctccgtcgag
     9121 aaaatccccg acgggccgac cacgaagctc atgcaggagt acgcgaagaa gtacaacatg
     9181 gtcatcgtcg tccctctgta cgaagaggaa gagaccggcg tgtactacaa caccgccgct
     9241 gtcatcgacg ccgatggcaa gtacctcggc aagtaccgca agaaccacat cccgcacgtc
     9301 gcgcccggtt tctgggagaa gttctacttc cgtccgggca acctcggata cccggtcttc
     9361 gagaccgcct acgcgaaggt cggtgtctac atctgctacg accgccactt ccccgaaggt
     9421 gcgcgcgagc tcgggctggc tggcgccgag atcgtgatga atccctcggc caccgtcgcc
     9481 gggctctccg aatatctctg gaagctcgag cagccggcgc acgccgtcgc caacggctac
     9541 ttcatcggcg ccatcaaccg cgtcggctac gaagcaccgt ggaacatcgg cgaattctac
     9601 ggacagagct acttctgcga tccgcgcggc cagatgctcg cggtcggccc acgcgacgcc
     9661 gacgccctcg tcaccgccga tctcgacatg gacaagatcc gcgaagtgcg gaacacgtgg
     9721 cagttctatc gcgaccgccg gccggagacg tacagcggga tcgtgcggct gtagatcttc
     9781 gtccgctaaa accttttcac gcggagacgc agagacgcga agggtttctc cgcacctccg
     9841 cgtctccgcg tgaaccgttt tccagaaatc atgcgcgacc acatcctcat ccacatcaac
     9901 ggcaaaccgc atcgcatcga tggcgacgcc gcattcgaga cactggcgaa ctatctgcgc
     9961 caggatgtcg ccgccaccgg cacgaagatc gtctgcgaag aaggcgactg cggcgcctgt
    10021 accgtgctca cgcggcgcag cgccaatgac gactaccgcg cggtcaactc gtgcatcttg
    10081 aatcttgcgc agctcgatgg ctcgtcgatc gtgaccgtcg aaggtttgcg cgaggagaaa
    10141 tcgctgcatc ccgtgcagga agcgatggtg caatgtcacg gcgcccaatg cggctactgc
    10201 acgcccggct tcgtcgtggc gatggccggc atgttcgaga cctgcgatcg ggtgaacgag
    10261 aaacaggttc gcgacggact gaccggaaat ctctgccgct gcaccggcta cgaaccgatc
    10321 atcaaagcgg cgctggccgt ggacggcgcg ccggcggcga agatgcgcga tcgttatccg
    10381 tcgctgccgg atgcgaacga ctccgtgcaa gtcggcacct tctgcgcgcc tgcgacgctc
    10441 gatgaggcga tcgcgttcaa acgcgagcat cccggctgca ccatcgtgca gggcggaacc
    10501 gacgtcggcg tttgggtaaa caagcgcaac tacgcggcgc ccgcgatgct gtcgttggcg
    10561 aagatcgatt cactcagcga gcttcacgaa gacgacggca cgatcgtcgt tggtgcgaac
    10621 acgacgctgg ccgactttga aacgttcatc gccgatcgca tccctgagct ggccggcatc
    10681 atggccatct tcggctcgcc gcagatcaag aacgcgggaa cgctggtcgg caacatcgcc
    10741 aacggctctc cgatcggcga cacgttgccc tacctcttcg tcgccggcgc catgttggag
    10801 atgaacggcg gcacccgctc cgtccccatc gaccgctttt acctcggata ccgcaagttc
    10861 gatctccaac ccgacgagat catcacgcgc gtacgcatcg gtatcgtccg cgacaagctc
    10921 aagctctaca aagtgtcccg ccgtaaagac ctcgacatct ccgccttcac cgccgcaatc
    10981 cgcctggcga tgaccggcga tcacatcgac cgcgccggag tcgcctacgg cggcgtggcc
    11041 ccaaccgtgg ttcgcctccc gcgcacggaa gcattcctca ccggcaagac agcgacgctc
    11101 gacaacttcg agcaagccgg ccgcatcgcc cgcgccgaga tcaaaccgat cagcgacgtc
    11161 cgcggctccg ccgactaccg gctgcaactg gccgagaaca tcatggctaa gttctggttc
    11221 gaggtattcg gccagacgtc cgcgccgcgg ctcgcgcacg cgtaggcgag cgaagcgagt
    11281 cccgcttcaa cgacgggtta agcagccgcg atcgggcgct tcgggaagac tgcgacggga
    11341 accggccgta gagcagccat gctgcgccgt ttcgccttga ttcgcgatac ctcatggagc
    11401 gtttcggcgg tgaagtaact ctcgccgcac ctcgggcagg aaaacatcgg aatgttctcg
    11461 attacgagga gagtcgcgcc gctaccgtag ctccttgtaa cgtagcgaag cgatacttcg
    11521 gcgtttccgc aaaacgcaca ggggtcactc gaggtagacc gtgatgacga ctgcgcgccc
    11581 gactgagtcg aacttcgcga caacgctgcc gatgccgttc gcgagcgtga tgccgcggat
    11641 gacgtacttt gcttcgcccg ttttgcggtc cctttgacgc tcgatgattt cgccggtgag
    11701 gacgatgctt tcgaggtcga ggatgtcgag ttcgtcattt tcaagttcat cggcggcgtg
    11761 ggaggaaacc aaatatcgat gctgccggac gaggtcgcga aggcgcggca gggtccggtc
    11821 ggccacttcg gcgtaattct accgcttctg tttctggctg gtctaaccgt tcccccgtgt
    11881 tcggggtctg aaccccataa cgctgctatc gcaaggggga ttattagctg gccagccgga
    11941 aactcagagg gagttgccgt cgcatgtgtc ttggcgccct ggatagagca tgcgcgaccg
    12001 gctgtcctac atgcatgaca cgcggatcca ccatggggac agacagttac gcgatgcgtg
    12061 accggtggtt acacgggtgc gggactccaa gttacacggt gtgggaccgt ggggtacatg
    12121 gtgtgtgacc atggggtaca cggtgtggga ccgtggggta cacggtgtgg gaccgtgggg
    12181 tacacggtgt gggaccgtgg ggtacacggt gtgggaccgt gg
//
LOCUS       DATLVW010000697         2378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_199174, whole genome shotgun sequence.
ACCESSION   DATLVW010000697 DATLVW010000000
VERSION     DATLVW010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199174"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /gene="allB"
                     /locus_tag="VNN25_20240"
     CDS             complement(<1..1201)
                     /gene="allB"
                     /locus_tag="VNN25_20240"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HXH93920.1"
                     /translation="MILRSRNVVTPQGIRPATIHVKGAVIDRVGEYDDVPDGGKVDDH
                     GELTLMPGVVDSHVHVNEPGRTEWEGFETATHAAAAGGVTTIVDMPLNSIPPTTSLSA
                     LETKVHALHGKCRVDVALWGGAVPGNADQLRPMLDAGARGFKCFLVDSGVPEFGHLDA
                     VGLAEALNALRGTGAPLLVHAELPEHLHAHGDDATSYASYLASRPHEAEDEAIALVYE
                     CVRNSGAKAHIVHLSSANGLETIRRAREENVPLTAETTPHYLYFDAEGVPDGATEFKC
                     APPIRERANRDALWRGIDEGLFLAIVSDHSPCTPELKRMNEGDIAKAWGGIASLQFGL
                     PIVWTEAKRRGVKFETIAELMTIGSAMLAGLDKRKGKLAAGYDADIVVFDPKGQFVVR
                     TELVQHRH"
     gene            complement(1506..>2378)
                     /locus_tag="VNN25_20245"
     CDS             complement(1506..>2378)
                     /locus_tag="VNN25_20245"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH93921.1"
                     /translation="MPTSTEKNNNTSATAASSGADLNGSAAADACRKIRARMAGVAAE
                     YFERVDGIEQSADEIIDEIVFENGEVFDRRNPAQRVPFPALVKMCHLERVSVGERGFY
                     STQGLDWNADKGCGAPFLYFTQGTAISEVEIDRFTGMMHVRRSDLLMDIGKPINPGID
                     RGQITGAFIQGMGWLTNEEMRYDAKGNLLSHSPTTYKIPNIQDVPPVFNVDWIDAENA
                     LNIRSSKATGEPPLVLAISVWMAVKNALSYLTAGGIPRLHAPATGEEILMRMTDYHHA
                     ARGADAREVAAPQL"
BASE COUNT          401 a          777 c          743 g          457 t
ORIGIN      
        1 tgtgccgatg ctgtacgagc tccgtgcgca cgacgaactg ccccttcgga tcgaagacga
       61 cgatgtccgc atcgtaaccg gcagcgagct tccctttgcg cttgtccaat cccgccagca
      121 tcgcggaacc gatcgtcatc agctcagcaa tcgtttcgaa tttcacgcca cgccgcttcg
      181 cctcggtcca gacgataggc aatccaaatt gcaacgatgc gatgccgccc caggcctttg
      241 cgatatcgcc ttcattcatc cgcttcaact ccggcgtgca gggtgagtga tcgcttacga
      301 tggcgagaaa cagtccctcg tcgatcccgc gccacagcgc atcgcgattg gcccgctccc
      361 ggatcggcgg cgcgcatttg aactctgtcg cgccgtccgg cacaccctcg gcgtcgaagt
      421 aaagatagtg cggcgtcgtc tctgccgtca gcggcacgtt ctcttcgcgg gcccgccgga
      481 tcgtctccag tccattcgca gacgagaggt gcacgatgtg cgctttcgct ccgctgttgc
      541 gcacgcactc gtagacgagc gcgatcgcct cgtcctcggc ctcatgcggc cgcgacgcga
      601 gatagctcgc gtacgaggtc gcgtcatcgc cgtgtgcgtg aagatgctcc ggcaactccg
      661 catggacgag cagcggcgcg cccgttccgc gcagtgcgtt caacgcctcg gccagaccaa
      721 cggcatcgag atgtccgaac tcaggaaccc cggagtcgac gaggaagcat ttgaagccgc
      781 gcgcaccggc atcgagcatc ggccgaagct gatccgcgtt tcccggaacc gcgccacccc
      841 acaaagccac gtcgacgcgg catttgccat ggagcgcatg aaccttggtc tccagcgccg
      901 acagggaagt ggtcggcgga atcgaattca gcggcatgtc gacgatggtc gtcactccgc
      961 ccgccgccgc cgcatgcgtt gccgtctcga acccctccca ctccgtccgc cccggctcgt
     1021 tcacatgcac atgcgaatcg acgacgcccg gcatcagagt cagctcgccg tggtcgtcaa
     1081 ccttgccgcc gtccggaacg tcgtcgtact cgccgacacg atcgatgacc gcgcccttca
     1141 catgaatcgt cgccggacga atgccctgcg gcgtaacgac attgcggctg cggagaatca
     1201 ccttcggatc atagcggcaa gggagtgcag gcgtctcgcc tgcggcggcg aggcgtctcg
     1261 cctcggcgtg gtttccgcga tgccggcggt ttttcgcatc gatgtgctaa tcgtgccggt
     1321 gtcgtactga gatggctgac cctttacggc tcattcaact tgccggcagt gattccgaag
     1381 gatcgagcga aagtcgtcgc gtcgacatcg tttccgcgac gctggacgtt cttcgcatcg
     1441 acgcgcatca ccgccgggcg agacgcccgc cggccgcagg cgagacgcct gcactccttg
     1501 cgtagttaca gctgcggtgc cgccacttcg cgggcgtcgg ctccacgggc ggcgtggtga
     1561 tagtcggtca tccgcatcag gatctcttcg ccggtcgctg gcgcgtgaag cctcgggatc
     1621 ccgccggcgg ttaggtagga gagcgcgttt ttcacggcca tccagaccga aatcgccaga
     1681 acgagcgggg gctcgccggt ggctttgctg gagcggatat tcagcgcgtt ttcggcatcg
     1741 atccaatcga cgttgaagac cggcggaacg tcctggatgt tggggatctt gtacgtcgtg
     1801 ggcgagtgcg agagcaggtt gcccttggcg tcgtagcgca tctcttcgtt cgtcagccag
     1861 cccatgccct ggatgaacgc gccggtgatc tggccgcggt cgatgccggg attgatcggc
     1921 ttgccgatgt ccatcagcag gtcgctgcgg cgcacgtgca tcatgcccgt gaagcggtcg
     1981 atctcgactt cggagatcgc cgtgccctgc gtgaagtaga ggaacggcgc gccgcagcct
     2041 ttgtcggcgt tccaatcgag accctgcgtc gagtagaaac cgcgctcgcc gacggaaacg
     2101 cgctcgaggt gacacatctt cacgagcgcc gggaacggga cgcgctgcgc aggattgcgg
     2161 cgatcgaaga cctcgccgtt ttcgaaaacg atctcatcaa tgatctcatc agccgattgc
     2221 tcgatgccgt cgacgcgctc gaagtattcg gcggcgacgc cggccatgcg cgcgcggatc
     2281 ttccggcagg catcggcggc ggcgctgcca ttgagatcgg cgccgctcga tgctgcggtg
     2341 gccgaagtgt tgttgttctt ctcggtggaa gtgggcat
//
LOCUS       DATLVW010000698         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_112568, whole genome shotgun sequence.
ACCESSION   DATLVW010000698 DATLVW010000000
VERSION     DATLVW010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112568"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VNN25_20250"
     CDS             complement(<1..233)
                     /locus_tag="VNN25_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93922.1"
                     /translation="MDENQIPATGERDEESTIGNNESAPPRIAHSDPRLPTPDPRTPS
                     HPPHPTPHPRKLALLAFTLALIVRLLNAPYIFE"
     gene            476..1657
                     /locus_tag="VNN25_20255"
     CDS             476..1657
                     /locus_tag="VNN25_20255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH93923.1"
                     /translation="MSTRCHESEVILDATHNAAKLDGCGDSSAPDLLWHLDRIDQQDG
                     ALDGLYRRHDLGAGTVVYVMDTGVLATHAEFANAAGDGSRVIAGFDVTRGVTIGSSHC
                     TSTNKSLAPCYSNTNELPGASHGTSVASLVAGKNVGVAPEALIVSIRVMNESALATTR
                     TYLEGLNRIISHSWDPATPQFRTAVVNISGWVLEKLNSNHDANPVTYATVEQKIRDMI
                     NGVDAMGHPDPNGKRFLFVVAGNNVDNGCGRTGVVDRFPATLGKEIDGLITVGGMTED
                     NSWWPGACHGGLEILAPSQGIFSASITAVDHYRGTKPNLRSGTSFAAPIISGIAARLL
                     SENPNLTPQELESMITATQSRIINPDASRADGKVAFVRDAPAVITAQHGVGPTIARMA
                     P"
     gene            complement(1728..2516)
                     /locus_tag="VNN25_20260"
     CDS             complement(1728..2516)
                     /locus_tag="VNN25_20260"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXH93924.1"
                     /translation="MPRALTVIVLLLAACAREPKAKSFVLATTTTVQGSGVLPMLIVE
                     FKRDSGIDIHPLVASTARALEFTAQHEADVAITHDPGAERRFLKTNGAKLYRQFMWND
                     FVIVGPPDDPADIAHARSAADAFRRIHEGHRVFLSRDDQSGTNTKERAIWRAALVVPE
                     FNPGYVKMGQPMPALLRSSSEMRAYTLSDRATFEQLADNLQLRLLFAGDPFLRNTYAV
                     ILPQPTGRPEAANAERFVDWLLNGRGHALLEKFEIKGKRAFTMI"
BASE COUNT          457 a          819 c          863 g          495 t
ORIGIN      
        1 ctttcgaaga tgtacggcgc gttgagaaga cgcacgatga gggcgagggt gaacgcgagg
       61 agagcgagtt ttcgggggtg gggtgtgggg tgtggggggt gggagggcgt gcgggggtcg
      121 ggagtcggga gtcgggggtc ggagtgggcg atgcgcggag gcgcggactc gttgtttccg
      181 atcgttgact cctcatcccg ctcgccggta gccgggatct gattctcatc cactaaggaa
      241 tgtggtgatt ccgagttcgc ggagagcagc cgctcgctcc gccccccgcc ccccgactcc
      301 cgacccccgc cggaaacccc caaagttttt cttgccattt tccccgcttt gcctgaatct
      361 tatttcgcgt cgtccaagac gttccaaaaa taaggtgatc ccgctgcgaa gaccgttcgc
      421 cgctttcctt tttgcgctcg ccgtttaccc cataacggcc ggagcgcagg atctcgtgtc
      481 tacgcgctgc cacgagtcgg aggtcattct cgacgcaacg cacaacgccg ccaagctcga
      541 tggctgcggc gactcctcgg cgcccgatct cctctggcat ctcgaccgga tcgatcagca
      601 ggacggcgcc cttgacggtc tctatcgccg ccacgatctc ggcgccggaa ccgttgtcta
      661 cgtgatggac acgggcgtcc tggccacgca cgccgagttt gccaatgcag cgggcgatgg
      721 aagccgcgtc atcgccggct tcgacgtgac ccgcggcgtc acgatcggct cgtcgcactg
      781 cacgagcacg aacaaatcgc tcgcgccgtg ttactcgaat acgaatgaac tgcccggcgc
      841 gtcgcatgga accagcgtgg cttcgctggt tgcgggcaag aacgtcggcg tggcgcccga
      901 agcgttgatc gtcagcatcc gggtgatgaa cgagtcggcg ctggcgacga cgcggacgta
      961 cctcgaggga ttgaaccgca tcatctcgca ctcctgggat ccggcgacgc cgcagttccg
     1021 gacggcggtg gtcaacatca gcggatgggt tctggagaaa ctgaactcga atcacgatgc
     1081 caatccggtg acgtatgcca ccgtcgagca gaagatccgc gacatgatca atggcgtcga
     1141 cgcgatgggc catcccgacc cgaacggcaa gcgctttctg ttcgtcgtcg ccggcaacaa
     1201 tgtcgacaac ggctgcggcc gcaccggcgt cgtcgaccgc tttccggcga cgctggggaa
     1261 agagatcgac ggcctgatca cggtcggagg gatgaccgag gacaacagtt ggtggccggg
     1321 agcgtgtcat ggaggcctgg agattctggc gccatcgcag gggatcttca gcgcatcgat
     1381 cacggcagtc gatcactacc gtggcacgaa gccgaacctc cgctccggga cgtcgttcgc
     1441 ggcgccgatc atctccggca ttgccgcccg cctgctatcc gagaacccga acctgacgcc
     1501 gcaggagctc gagtcgatga tcaccgccac gcagtcgcgc atcatcaatc ccgacgccag
     1561 tcgcgccgac ggcaaggttg ccttcgtacg tgatgcgccc gcggtgatca ccgcgcagca
     1621 tggggttggg ccgacgatcg cgcgcatggc cccgtgattc atgcacgcga agacgcgaag
     1681 atagcttcaa acacttcgcg tcttcgcgcc ttcgcgtgag agcttgatca tatcatcgta
     1741 aacgcgcgct tgcctttgat ctcgaacttc tccaacagcg cgtgcccgcg cccgttcagc
     1801 agccaatcga cgaaacgttc cgcgtttgcg gcttcgggtc ttcctgtggg ttggggaagg
     1861 atgacggcgt aggtgttgcg gaggaaggga tcgccggcga agagcaggcg gagctgaagg
     1921 ttgtcggcga gttgctcgaa cgtggcgcgg tcgctgagcg tgtaggcgcg catctcgctc
     1981 gatgaacgaa gaagcgctgg catcggctgg cccatcttga cgtagcctgg attgaattcg
     2041 ggtacgacca gtgccgcccg ccagatggcg cgctccttcg tgttcgtccc cgactgatca
     2101 tcgcgcgaca ggaaaacgcg atggccttcg tggatgcgcc gaaatgcatc ggcggcactg
     2161 cgggcgtgcg cgatgtctgc gggatcgtcg ggcggaccga cgatgacgaa gtcgttccac
     2221 atgaactggc ggtagagctt ggcgccgttc gtcttgagga accgccgctc ggcgccggga
     2281 tcgtgcgtga tcgcgacgtc ggcctcatgc tgcgccgtga actcgagcgc gcgtgccgtg
     2341 ctggcgacaa gcggatggat gtcgatgccg ctgtcgcgtt tgaattcgac gatcagcatc
     2401 ggcaggacgc ccgatccctg caccgttgtc gtcgtggcca ggacgaatga tttcgctttc
     2461 ggctcccgcg cgcatgcggc gagcagcagc acgattacgg taagtgcacg gggcatcgct
     2521 atttcttcaa cgccgcgctg ccgccggcgc ggaactcctc ttcgttcagt tgcaggtcat
     2581 cccgcggcgg cccgtcgccc atgaccccgg cgtagacctc actcgcagtc tcgc
//
LOCUS       DATLVW010000699         4745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_318535, whole genome shotgun sequence.
ACCESSION   DATLVW010000699 DATLVW010000000
VERSION     DATLVW010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318535"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1745
                     /locus_tag="VNN25_20265"
     CDS             63..1745
                     /locus_tag="VNN25_20265"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXH93925.1"
                     /translation="MVRTFAFFTLFALAGCASAPPMVVAIHTVPPKFNIVPAKTLAVL
                     GDADNAADQKREDDFIDLVITKLRRYEQYDVKDERQFSRGLNPGHGLFEAEDWQKYLQ
                     QTAGEVIVRVGVPNTGCPMTETTRDDGSDDAGDVVWVDVECRATLNLFKPRTGERIGS
                     VNADGVGSDARQHTAWNDAMNDAADQIIGGFAPEMSADAIAIDQDAPLAHEGMVKFFS
                     SDYAGARSLWENGLANAPNSAPLLYNLGALCEALHDPDAGRTYYLRAIAIAPQVQRYH
                     EALAQLDRRRADAEEAAKDPAVEDAKKSVAASAQAAQRTAMAVNFSGIPYLPIDAGTF
                     TMGCSKGDIDCDENEKPAHAVTISKPFYLAKTPATNAQYQQCVDAGACHGSADAQKAL
                     HPVVNVDWNDARAFCAWAGGRLPTEAEWEYAARGGIEGWRFPWGNSAGKGNANLADNG
                     GRHVEALGIEPTAAAISYPFLGTTPVGTFNPNGFGLDDMTGNVSEWTADWNGYYDPGT
                     LTDPSGPAAGEQRVVRGGSWVDTGAGARISVRYNAVPGFAQSTVGFRCAIDR"
     gene            1942..2220
                     /locus_tag="VNN25_20270"
     CDS             1942..2220
                     /locus_tag="VNN25_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXH93926.1"
                     /translation="MTFTLPGLIIYIIIAAICGAIGKAIAGGVRGGLIVSIALGFIGA
                     ILGPWVAHKLKLPEPFMVQVSGHPFPVLWSIIGAALFVAIVHLFSRRR"
     gene            complement(2233..4071)
                     /locus_tag="VNN25_20275"
     CDS             complement(2233..4071)
                     /locus_tag="VNN25_20275"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HXH93927.1"
                     /translation="MVRNILALLLLAASAHADENALLDRWIAAAGGRQRLESVAAVHR
                     VDAIDEDGMPGVREEWTTAALQRRERLAHERDEAITVYDGTNGWRRDWNGFVENLAGS
                     DLRTQSDLAMIHSFAALTGVAGAPSIIDENTLEFHAKSGSAVRFVLDHETALPLRAEM
                     PSFDGIRTVAFSDWRKVDGVAIAFGETWTTGPNKTTAHLQSIEFVPRDRVDLTKPSPG
                     PEDAFYLRNGARSETLPFNFDNNHIMVLTTVNGAGPIWFLVDTGANYSIINQSRVSEF
                     HLTPYGGLTTIGGGSAATSGSYVDHVTYRLGEVELRDQHAAVLELRGLEKLYGMQLGG
                     LLGYDFLSRFVTDIDYVAKTLTLRPRTFDTSRISGGRVPLVMQGEQPYFDGSIVVKGE
                     TIPAWFILDVGAADTVTFTTPFIAEHHLLEGAGDAERTVHKFSAPDVEAFNPTNIRGL
                     MDAIVIDGVTLPHVLVNLSAAKSGAYTSPAFSGNIGETILSRFAHVILDYGRNVMILQ
                     PQPSTTNPFEERKTFGMTIIAGSPDLHHFTISAVGADSPSAVAGFQKGDVITAIDGAP
                     AETWNLAGIRKVFTSEGTAHDVAIMRGTQEVKIHTTIAMTPVSGLR"
     gene            4182..4511
                     /locus_tag="VNN25_20280"
     CDS             4182..4511
                     /locus_tag="VNN25_20280"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HXH93928.1"
                     /translation="MKSRLVWTSDPEAAKRLREEGAVDARADQDPSKQTIRVELDRKR
                     RAGKSVTVAGGFQLTAASLADLAAMLKKRCGAGGTAGDSEIEIQGDHLARVTAELERL
                     GYKVRKS"
BASE COUNT          906 a         1456 c         1533 g          850 t
ORIGIN      
        1 gtccgtgatc gacggaggca ctggctcagc aatatggaca acaggacgta ttacccgatg
       61 aaatggtcag gacgttcgcg ttcttcaccc tcttcgcact ggccggttgc gcgtcggcgc
      121 cgccgatggt tgtcgcgatc cataccgtgc cgccgaagtt caacatcgta ccggcgaaga
      181 cactggccgt actcggcgac gcggacaacg ctgccgacca gaagcgggaa gacgacttca
      241 tcgatctggt catcaccaaa ctgcgccggt acgagcagta cgacgtaaaa gacgagcgcc
      301 agttctcccg cggcctcaac ccgggacatg gcctcttcga ggccgaggat tggcagaagt
      361 atctgcagca aaccgccggc gaggtgatcg tcagggtggg ggtgccgaat acagggtgcc
      421 cgatgaccga gacgacgcgc gatgacggca gcgacgacgc tggcgatgtt gtgtgggttg
      481 atgtcgagtg ccgggccacg ctcaatctgt tcaagccccg gaccggtgaa cgaatcggca
      541 gtgtgaatgc agacggagtt ggctctgacg cccgtcagca cacggcatgg aacgatgcca
      601 tgaacgacgc ggccgatcag atcatcggtg gcttcgcgcc agagatgtct gctgacgcca
      661 tcgcgatcga ccaggacgcg ccgctggccc acgaaggcat ggtgaagttc ttcagcagcg
      721 attacgccgg tgcccgctcg ctatgggaaa acggtctggc gaacgcaccc aactctgcgc
      781 cgctgctcta caacctcggc gcgctttgtg aggcgctgca cgacccggac gcagggcgca
      841 cgtactattt gagagcgatc gccatcgcgc cacaggtgca gcgttaccac gaggcgctcg
      901 cgcaactcga tcggcgccgt gccgatgcgg aagaagcggc caaagatccg gcagtggaag
      961 atgcgaagaa gagcgtggca gccagcgcgc aggcggctca gaggacggcc atggccgtca
     1021 acttctcggg tatcccatat ctgccgatcg atgccggcac gttcaccatg ggctgtagca
     1081 aaggcgatat cgattgcgac gaaaatgaga agcccgcgca cgcggtgacg atcagcaagc
     1141 cgttttacct ggccaaaacg ccggccacga acgcgcaata ccagcaatgc gtcgatgcgg
     1201 gcgcgtgcca tggcagtgca gacgcgcaga aggcgctcca tccggtagtt aacgtcgact
     1261 ggaatgacgc gcgcgccttc tgcgcatggg cgggtggacg gctgccgacc gaggctgagt
     1321 gggagtacgc ggcgcgcggc ggcatcgagg ggtggcgctt cccgtggggg aattcggctg
     1381 gcaaaggaaa cgcgaatctc gccgacaacg gcggccgcca cgtcgaagcc ctcggcatcg
     1441 aaccaactgc cgccgccatc agctatccgt ttcttggcac caccccggtc ggtacgttca
     1501 acccgaacgg cttcggtctc gacgacatga ccggcaacgt ctcggaatgg accgcggatt
     1561 ggaatggcta ctacgatccc ggcaccctaa ccgatccttc cggtccggcc gccggcgagc
     1621 aacgcgtcgt gcgcggcggc tcctgggtgg atacgggtgc aggcgcccgc atttccgtcc
     1681 gctacaacgc ggtgcccggg tttgcgcaaa gcaccgtcgg cttccgctgt gcgatcgatc
     1741 gatgattcat gtcttctgac gcgaaggcgc tcacgcgaag acgcgaagac gcgaagaaaa
     1801 acccggtggt tcaccagctt cgcgtcttcg cgccttcgcg tgagcccatg gctagagacc
     1861 gctggcatca atgttgcggc tgaccaacgg ttgagctccg actaactttg cgaagcgaga
     1921 ctgtgcgaaa ggactttgcc catgaccttc acgttgcccg ggctgatcat ctacatcatc
     1981 attgctgcaa tctgtggtgc gatcggtaaa gcgatcgccg gcggagttcg cggcggcctt
     2041 atcgtctcga tcgccctcgg ctttatcggc gccatcctcg ggccgtgggt ggcgcacaag
     2101 ctaaagctgc cggagccttt catggtccag gtcagcggac accccttccc ggttctctgg
     2161 tcgatcatcg gcgccgccct gttcgtcgcg atcgtccacc tgttctcccg tcgaaggtga
     2221 cgcggcgacg ccttaccgaa ggccggacac cggcgtcatc gcaatcgtcg tgtggatctt
     2281 cacttcctgc gtgccccgca tgatggcgac gtcgtgcgcg gttccctcgc tcgtgaatac
     2341 cttgcggatg ccggcgaggt tccaggtttc tgccggtgcg ccatcgatcg cggtgatgac
     2401 gtcgcctttt tggaatccgg cgacggctga cggtgagtcg gcgcccaccg cactgatcgt
     2461 gaagtggtgc aggtcggggc taccggcgat gatcgtcatt ccgaacgtct tgcgctcctc
     2521 gaacggattc gtggtcgatg gctgcggctg caggatcatg acgttgcggc cgtagtcgag
     2581 gatgacgtga gcgaatcgcg agaggatcgt ctcgccgatg tttccggaaa aggcgggact
     2641 ggtgtaggcg ccggactttg cggccgacag gttcaccagc acatgaggca gcgtgacgcc
     2701 gtcgatgacg atcgcatcca tcagaccccg gatgttcgtt ggattgaacg cctcgacgtc
     2761 gggcgcgctg aatttgtgca cggtccgctc cgcatcgccg gcaccctcga gaaggtgatg
     2821 ctcggcgatg aacggggtgg tgaaggtgac ggtgtcggcg gctccgacgt cgaggatgaa
     2881 ccaggccgga atcgtttctc ccttcaccac gatgctgccg tcgaaatacg gctgttcgcc
     2941 ctgcatcacc agcggcacgc gccccccgga gatccgcgac gtatcgaacg tccgcggacg
     3001 cagcgtcagg gtcttggcca cgtaatcgat atcggtgacg aagcggctga ggaagtcgta
     3061 gccgaggaga ccgccgagct gcatgccgta gagtttttcg agaccgcgca actccaggac
     3121 cgcggcatgt tgatcgcgca gttccacctc gccgaggcgg taggtgacgt ggtcgacgta
     3181 ggaaccggat gtcgcagcac tgccgccgcc gatcgtcgtc agtccgccat acggtgtcag
     3241 atgaaactcg ctcacacggg actggttgat gatcgagtaa ttcgcgccgg tgtcgacgag
     3301 gaaccagatt ggcccggctc cgttgaccgt cgtcagcacc atgatgtggt tgttgtcgaa
     3361 gttgaacggg agtgtttcgg accgcgcgcc gttgcgcaga tagaacgcgt cctccgggcc
     3421 gggtgatggc ttcgtgagat cgacgcggtc gcgcggcacg aattcgatcg attgcagatg
     3481 tgcggtcgtc ttgtttggtc cggtggtcca cgtctcgcca aaggcgatgg caacgccgtc
     3541 gaccttccgc caatcggaga atgcgaccgt tcgaattccg tcgaaggatg gcatctctgc
     3601 gcgcagcggc agcgcagtct cgtgatcgag cacgaagcga accgcggagc cgctcttcgc
     3661 gtgaaactcg agcgtgtttt cgtcgatgat cgaaggcgca ccagcgactc cagtcagcgc
     3721 ggcgaacgaa tggatcatcg ccagatccga ctgtgtacga aggtcagagc ccgccaagtt
     3781 ctcgacgaag ccgttccagt cgcggcgcca gccgtttgta ccgtcgtaca cggtaatcgc
     3841 ttcatcgcgc tcatgcgcca gccgttcgcg ccgctgaaga gccgcggttg tccactcttc
     3901 ccggacacca ggcatcccat cctcgtcgat ggcgtcgacg cgatgaaccg ccgcgaccga
     3961 ctccaaccgc tgacggccgc ccgcggcggc aatccaacga tcgagaagag cattctcgtc
     4021 cgcatgggcc gaggcggcga ggagcaggag ggcgagtatg tttcgaacca tgatcttggc
     4081 gtcaggctac tgcatctcgg cggacggaat cgagtgatcg gttcgacgag atgcctggtc
     4141 cttgctggca gggacgccac gcgccacaat caacgctgcc gatgaaatcg cgcctcgtct
     4201 ggaccagcga tcccgaagcc gcgaagcggc tgcgcgagga aggcgcggtc gacgcgcggg
     4261 ctgaccagga tccgtcgaaa cagacgatcc gcgtggagct cgatcgcaag cgccgcgctg
     4321 gcaagagcgt gacggtcgca ggcggtttcc aactgacggc tgcatcgctg gcagacctgg
     4381 cggcgatgtt gaaaaaacga tgcggtgccg gcggcacggc gggagacagc gagatcgaga
     4441 ttcagggcga tcacctcgcg cgcgtaaccg ccgagctcga gcgcctcggc tacaaggttc
     4501 ggaagagctg aatgcgcgaa ggcgtctcac gcgaagacgc tcacgcgaag acgcgaagac
     4561 gcgaaggggg aaaccaccag ctgcttttcc ttcgcgtctt cgcgccttcg cgtgagacgc
     4621 ctcgccgttg tgagagatcg cgatcctctg cttctttccg cagtactcca gccactgctc
     4681 atccgatccg tcgggcgcaa aaagatcccc gtgacgttcc acctcgagac cggctgagga
     4741 aagaa
//
LOCUS       DATLVW010000700         4728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_28811, whole genome shotgun sequence.
ACCESSION   DATLVW010000700 DATLVW010000000
VERSION     DATLVW010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28811"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VNN25_20285"
     CDS             <1..892
                     /locus_tag="VNN25_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93929.1"
                     /translation="QPAEATMDDWHALLPARSARSTFPCAGDRLCVRHYSFDKDAIVK
                     LPAISEHAICLVVGRTAEFAIDAGHCWNRRIYRPGEGSLIPAGTAVSFRVIGSAAVAH
                     LYLSGEWFDKVATAGLDFEPGDIRLSGSTRLRDPFLWIFMSGLLRRQRLGQRLDRVIL
                     DSAILGLACHLLREYVLQDKFPLTNAWEDPARVGDFIDELVGIELGLHELAESIGWPA
                     QNLLRDFASAEAARLRKWLQHSRIRRAHALLTNSTARLEDVALAAGIATPEALDVISY
                     FATGYPAAELRDLCNVSSAA"
     gene            complement(903..2312)
                     /locus_tag="VNN25_20290"
     CDS             complement(903..2312)
                     /locus_tag="VNN25_20290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH93930.1"
                     /translation="MALPSGQTTSANGRVVRSVSAAHHVEAVAAVVHGEFSQSESSSI
                     EVEAVSSSWRRSAQAHHVNPESRESPRILTESSLRSSIEPIENVVLAAQTELDRLHRM
                     AGQAGYVTLLCDQNGIAIDYRGNDQRSDEFRHWGVWRGGVWSEDIEGTNGIGTCIAEC
                     RPITVHQSQHYRARHIGLSCSGAPIFDSDARLVAVLDVSSIDPDLSAQAHALTLPLVT
                     TSARMIEERLFRERFRKEWIIAVAPHDEQPASLLAVDRDHRVVGADRNARAQYRIDSQ
                     SMDAGVSVWAIFSRKGLVLRKGVPTDCPARLTTVSGGDNFFALVSSPVTTSRLHIGKL
                     EATLLMQPRIDLLEDLSRRFTIKPPRGGLAPGALRRVTEYMDSHLCEELALESLAAHA
                     GLSTSHFARAFKQSVGVPPHRYLLEQRVKKAGDLLKQTQEPLTAIAQSLGFADQSHFS
                     RSFHWLVGLAPSEFRRSHR"
     gene            complement(2482..3918)
                     /locus_tag="VNN25_20295"
     CDS             complement(2482..3918)
                     /locus_tag="VNN25_20295"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXH93931.1"
                     /translation="MRAWRSLIELRIDDALATLAQFEDEIARADDSVAPRSREFAKVL
                     RAVLLVLKSQDGATVCTALAVLENRHRLGGKSPALAAALRVGYWKVRDFDRYYAVPRL
                     KHAVPTHRSTHGLATIIGPTFEAVVEAEQLRLAVATRLVRSAHERAVSRFGKRSPVTA
                     RAAVVLAELLYEEGHCDGLEALVMESLAEVRTSGDDESAMRGYRVLARLAARRGDTEF
                     AFLILKEAEVLATARDWPGMMAESLMLRTQLLLKEGRTRDAANCTERIGTLARDFSSD
                     DALQASLALARAQLLVATGDARRGATILRELKAVSSCKRNNYWALQLSVQLADALLGC
                     GDQAEGRAMLIQSLQVGARAGVYQTFVDAGDRVAEFLLTLHHATPQDSRMPPELRPYV
                     GSILADRAQQRTRVPPTRASRVTESLSPREHSVLRSMSYGLSNKRIAQELQIAPETVK
                     SHVKGIFIKLAVQTRAHAVSTAGALGLL"
     gene            complement(4265..>4728)
                     /locus_tag="VNN25_20300"
     CDS             complement(4265..>4728)
                     /locus_tag="VNN25_20300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH93932.1"
                     /translation="SLSALGRELRKGIDPPSKPQYRAVVEGRPRELTAVVRDEAYRIA
                     REAVSNAYQHAKAGHIETEVTFGDAELTIRVRDDGIGVDPEVLARGQRPGHWGLPGMR
                     ERSESLGGQLNVWSERNAGTEVELVIPADMAYSEPRRSSFTRLKDLLRSCR"
BASE COUNT          902 a         1446 c         1410 g          970 t
ORIGIN      
        1 gcaacccgct gaggcgacta tggatgattg gcacgcactg ctgcccgcgc ggtcggcgcg
       61 ttcaaccttc ccgtgtgccg gagaccggtt gtgcgttcgg cattacagct tcgataaaga
      121 tgccatcgtc aagttgccgg ccatttcgga gcacgcaatt tgcttggtgg tcggccgaac
      181 tgcggaattc gcgattgacg cgggccattg ctggaatcga cgcatctacc ggccgggcga
      241 ggggagtctc atccccgcgg gaaccgccgt gtcgttccgt gttatcggct cagccgcggt
      301 cgcacattta tatctcagcg gcgagtggtt cgacaaggtc gcaaccgcag ggttggattt
      361 cgagccgggc gacatccggc tctccggatc tacgcgactt cgcgatccat ttctttggat
      421 attcatgtca gggctccttc gccgccagcg cttagggcaa cggcttgacc gtgtgatact
      481 tgactcggca atcctggggc tagcttgtca cctccttcgt gagtatgtgt tgcaggacaa
      541 gtttccactc acaaatgcgt gggaggaccc cgcgcgcgta ggcgatttca ttgacgaatt
      601 ggttgggatc gaattgggac tgcacgaact cgctgaaagc atcgggtggc cggcgcagaa
      661 cctcttgagg gactttgcaa gtgcggaggc ggcccgccta cgcaaatggc ttcagcactc
      721 acgcattcgg cgggcgcacg ctcttctgac caactcaacc gcgcgactcg aagatgtagc
      781 cctcgccgcg ggaatcgcga caccagaagc actagatgtt atttcctact ttgccaccgg
      841 ctatccagcg gccgaacttc gagatctgtg caacgtgtca tcggccgcgt agtccgcaga
      901 cactaccggt gagatctacg gaactcgctt ggtgctagtc ccacgagcca gtgaaaactt
      961 cgggagaaat ggctctgatc ggcgaacccg agcgactgcg caatggctgt tagaggctct
     1021 tgggtttgct tcagcaaatc ccctgccttt ttaactcgtt gttccaacag gtacctatgc
     1081 ggcggcacgc ccacgctttg cttgaacgct cgagcgaagt ggctcgtgga cagtcccgca
     1141 tgtgcggcga gagactcgag cgcaagttct tcgcacagat gggagtccat atattccgtc
     1201 acgcgtcgaa gtgctccagg agccaaaccc cctcgtggcg gtttgatcgt aaagcgccgg
     1261 ctcaggtctt cgagcaaatc gattcgtggc tgcatgagaa gtgttgcttc caatttgccg
     1321 atatgaagcc gggatgtggt caccggactt gaaaccagag caaagaagtt gtcgcctcca
     1381 ctcactgtcg tcagacgcgc cggacagtca gtgggtacgc cttttcgaag caccaaccct
     1441 ttgcgtgaga agatcgccca gacgctcacc ccggcgtcca tagattggga atcgatgcgg
     1501 tactgggctc gcgcattgcg atctgctcct accacgcgat gatcgcggtc caccgccaac
     1561 aatgatgcgg gttgctcgtc atgcggcgct acggcgataa tccactcctt gcgaaatcgc
     1621 tctcggaata accgctcctc gatcatgcga gccgacgtcg tgacaagcgg aagtgtgagt
     1681 gcgtgagcct gcgcggaaag atcagggtca atcgatgaca catcaagaac cgcgaccagg
     1741 cgcgcgtcag aatcaaagat aggtgcgccg gaacaactga gcccgatgtg tctcgcccgg
     1801 tagtgctgag attgatgcac cgtgatcgga cgacactcgg cgatgcaggt gccgatgcca
     1861 ttggtacctt caatgtcctc cgaccacact ccacctcgcc acactcccca atgccgaaac
     1921 tcatctgaac gttgatcatt gccacggtag tcgatcgcga tcccgttctg gtcacagagc
     1981 aaagtgacgt agccggcttg ccccgccatt ctgtgcaggc gatcgagttc tgtttgagca
     2041 gccaaaacga cattttcgat tggttcgatg gagctgcgca gcgaactctc cgtgaggatc
     2101 cgtggactct cgcggctctc cggattgaca tggtgggctt gcgcactgcg ccgccaagag
     2161 cttgaaaccg cttctacttc gatgctcgac gattcgcttt gagagaattc gccgtgcact
     2221 actgcagcga cggcttctac gtgatgcgcc gcagaaacgc tgcggacaac ccgcccgttc
     2281 gctgatgtag tctgtccgga cggtaatgcc atacggctgt cattttttgg acaactcagc
     2341 aaatcatatc atcggcctga ggaatatgcg cgccgcacgc cacgcctctt ctaggagttg
     2401 cacactcatt ccgcgttgca gcgaagcttc gagtcagtca acagtcagtg gatgtagatt
     2461 ttaccagcgt ggcaccgtcg ctcagagcaa acccaaagcg ccggccgtcg atacggcatg
     2521 agcacgcgtc tgcacggcga gcttgatgaa gatgcctttg acgtgggact tcacggtttc
     2581 gggggcaatc tgcagctctt gcgcgatgcg tttgttggaa agcccatagc tcatcgagcg
     2641 caggacgctg tgttcgcgcg gacttaagga ttccgtcacc cgcgatgcac gagtcggcgg
     2701 cacacgtgta cgctgttgag cccggtccgc gagaatgctt ccgacgtagg gtctgagttc
     2761 cgggggcatg cggctgtcct gcggggtggc gtggtgaagg gtcaagagga actccgccac
     2821 gcgatctccc gcgtcgacga acgtctgata caccccggcg cgcgcgccaa cctgaagcga
     2881 ttgaatgagc attgcacggc cttcggcttg gtctccgcac ccgagcagag cgtcggcgag
     2941 ctgaaccgac aactgcagtg cccagtagtt gtttcgcttg cagctgctca ccgctttaag
     3001 ctcccgcagg atcgtcgctc ctctgcgcgc gtccccggtg gcgaccagca gctgtgcacg
     3061 tgccaaggcc aggctggcct gcaacgcgtc gtccgaagag aagtcgcgcg cgagtgtccc
     3121 aatgcgctcg gtacagtttg ctgcgtcgcg cgtgcgaccc tccttgagga gaagctgagt
     3181 gcgcagcatc aagctctccg ccatcatccc cggccagtcg cgtgccgtcg ccaggacctc
     3241 cgcctccttc aggatgagga aggcgaactc cgtgtctcct cgacgggcgg cgagtcgagc
     3301 cagcacccga tatcctcgca tggcgctctc gtcgtccccc gacgttcgga cctctgcgag
     3361 gctttccatg accagagcct ccaggccatc gcaatgtcct tcctcgtaga gcaactctgc
     3421 aagcacgacg gccgctcggg ctgttaccgg tgatcgcttc ccgaagcgac tcacggcgcg
     3481 ttcgtgcgcg cttctcacga gcctcgtcgc gacggccagt cgcagctgtt cagcctccac
     3541 gaccgcttcg aacgtcggcc cgatgatcgt cgcaagcccg tgcgtgctgc ggtgagtcgg
     3601 caccgcgtgc ttgagacgag gcaccgcgta gtatcgatca aagtctcgca ccttccagta
     3661 gccgacgcgc aacgctgccg ccagcgccgg actctttcct cccaatcggt gccggttttc
     3721 gagtacggca agcgccgtgc acaccgtggc gccatcctgg ctcttcagca caagcagcac
     3781 ggctctcaag accttggcaa actctcgaga tcggggcgca accgagtcat cggcacgcgc
     3841 gatctcatct tcgaactgtg cgagcgtcgc caaggcgtcg tcaatccgca gctcgatgag
     3901 gctgcgccaa gcgcgcacga gcagagtgtt tggggaatgg cgggcgcttc gtgattccag
     3961 cgcgctgagc tccgagcgtg gcctggtatt cgggttccca agcgcggtac gccttgggcc
     4021 agagtcccgg ttcagggcag cggaatcgtg ctgccggaca actgcgagag atgattgacg
     4081 cacagcgtga attgagcctc gcgaagatgt ggatcgttcg tcaaacaggc agctgttgga
     4141 caccgtgtgg ttagttcgga acgagagtag gacgcggcgg ctcacggcaa gctcacggga
     4201 tggacgatgg gaccgtgggg gcccgggagc tcagtaaccg gatgtgtcac cgacttggcg
     4261 ttcgtcagcg gcaggagcgg agcagatctt tgagtcgggt gaatgaggat ctgcgcggct
     4321 ctgaataggc catgtcggct ggtatgacaa gttcaacctc ggtacccgca tttcgctcac
     4381 tccacacgtt gagctgaccg cccagactct cgctccgttc gcgcatccct ggcaatcccc
     4441 agtggccggg ccgctgacca cgtgcgagga cctcgggatc cacccctatg ccatcgtcgc
     4501 gcactcggat cgtgagttcg gcatcgccaa aagtgacttc ggtctcaata tggccggcct
     4561 ttgcgtgttg atacgcgttg ctgaccgctt cacgtgcaat gcgataagcc tcgtcacgca
     4621 cgacggcagt caactcgcgg ggcctgccct ccacgactgc gcggtactgc ggctttgacg
     4681 ggggatcaat ccccttccta agttcccttc cgagggcgct caacgaaa
//
LOCUS       DATLVW010000701         3995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_112697, whole genome shotgun sequence.
ACCESSION   DATLVW010000701 DATLVW010000000
VERSION     DATLVW010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112697"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VNN25_20305"
     CDS             complement(<1..286)
                     /locus_tag="VNN25_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93933.1"
                     /translation="MQHLTEEQLVAHYYNDGDAVTAEAHLATCEECRAQLATLRDVLA
                     LIDQLPIPEKNAAYGEEVWTRLRWKLGSERRRSRVWGTISAIAAALALAFI"
     gene            complement(274..855)
                     /locus_tag="VNN25_20310"
     CDS             complement(274..855)
                     /locus_tag="VNN25_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH93934.1"
                     /translation="MTTDLAVVSRARSGDPEAFRMLVESHSKAAFRVAYRMTGNEHDA
                     DDVVQETFLRAYKQLQHFEERANFGTWLHRIVINCSLDLLRARSRADKHYGGDFESAE
                     LAGASGARSEAQQDRLMISAELQTQVATAMKRLSANERTAFILRHFEGMAVEEIGKTL
                     GIQVSATKHTIFRAVRKLRESLEPFVRSMPCNT"
     gene            complement(1096..1293)
                     /locus_tag="VNN25_20315"
     CDS             complement(1096..1293)
                     /locus_tag="VNN25_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HXH93935.1"
                     /translation="MKKIIAVVLLAGATLASGCATRAVLVAPVPRVEVIGVAPYRGAV
                     WIPGHYIRAHRDWVWVRGYWR"
     gene            1384..2190
                     /locus_tag="VNN25_20320"
     CDS             1384..2190
                     /locus_tag="VNN25_20320"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXH93936.1"
                     /translation="MTSLVHYVLLYGVPLIFINVLLEQLGAPVPAVPALIVAGALSRD
                     GKLSSTHVLLAALFASLIADYIWFLLGRRFGYRILRTLCRISLSPDSCVRDTEARFER
                     WGLKSLLIAKFIPGFSTVAPPLAGASGRSTLAFVIYDGIGALIWAGTAVAAGRAFHRA
                     IDRVLLALENLGWWAIIILGSLLALVIVVKWAQRIQFIKQLRTARIQPQELKAMLDRG
                     ELPVVLDVRTPGARKHDPRSIPTAILALLSDIDAQLGDLAPHQEVVLYCT"
     gene            2530..3348
                     /locus_tag="VNN25_20325"
     CDS             2530..3348
                     /locus_tag="VNN25_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93937.1"
                     /translation="MTITETARDTFLAPETRDFYIESMRLMRDGGVPFLVGGAYAFAR
                     YTGIERHTKDFDVFLRRDDFPKAKRVFEAAGYESELNFPHWLGKAIKGDDFVDLIFSA
                     GNGVAVVDELWFDFAVDDEVFGVDVKLIPAEEMIWSKGLIMERERFDGADVAHVLLAT
                     GDRLDWRRLIDRYGKHWRALYAHLVLFGFSYPSERSRVPAWVMTELNARIADETNQPD
                     APEKVCYGTILSRQQYLADINEWGYQDGRLRPIGNMTAEEIAQWTAGIEVDGSK"
     gene            3345..>3995
                     /locus_tag="VNN25_20330"
     CDS             3345..>3995
                     /locus_tag="VNN25_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXH93938.1"
                     /translation="MKETLRIAAVADIHVKKTGQGSMQSLFAAASEQADVLLLCGDLT
                     DYGLVEEAKVLAREITASLRIPVIGILGNHDFESGKQDDVVRIMLDAGVTMLDGDSHE
                     IEGVGFAGVKGFGGGFGRRQLGAWGEDIIKKFVHETIEEALKLEQALAKLRTPQKIAV
                     IHYSPIHATVVGEPEEIYPFLGSSRLEDPIDRYRCNAVFHGHAHRGFLEGRTKGNIP"
BASE COUNT          733 a         1206 c         1234 g          822 t
ORIGIN      
        1 cgatgaaggc cagcgccaga gccgcggcga ttgcgctgat cgttccccag acgcgagagc
       61 ggcgccgttc gctgccaagc ttccagcgga gccgcgtcca gacttcttcg ccatatgcgg
      121 cgttcttttc ggggatcgga agctgatcga tgagcgcgag gacgtcgcgc agcgtggcca
      181 gttgcgcgcg gcactcctcg cacgtcgcca gatgcgcttc ggcggtcacg gcgtcaccgt
      241 cgttgtagta gtgagcgacc agttgttctt ctgtcaggtg ttgcatggca ttgacctcac
      301 gaacggctcc aacgactcac ggagcttgcg taccgcgcga aaaatcgtgt gtttggtggc
      361 actgacctgg atgccgagcg tcttgccgat ctcctcgacc gccattcctt cgaagtggcg
      421 gaggatgaag gcggtccgct cgttggcgct gagccgcttc atcgccgtgg caacctgtgt
      481 ttgcagctca gcggagatca tcaggcgatc ctgttgcgct tcgcttcgcg cgcccgaggc
      541 tccggccagc tcggcggact cgaaatcgcc tccataatgt ttgtcggcgc ggcttcgtgc
      601 acgaagcagg tcgagcgaac agttgatgac gatgcggtgc agccacgtgc cgaagttcgc
      661 gcgctcctcg aaatgctgga gttgcttgta ggcgcggaga aaggtctcct gcactacgtc
      721 gtcggcatca tgttcgtttc cagtcattcg ataggccacg cgaaacgctg ctttggaatg
      781 actctcgacc agcatccgga acgcttccgg gtcgccggat cgcgctctgc tgaccacggc
      841 gaggtcggtc gtcatctcca tccggccgat tagactagaa aagtggggtt tggttagccg
      901 ggaggcggga ccggttctct gcgtctccgc ggcttccaca caaaacaatg aaccacagag
      961 acactgaggt cacagagttg cgatggttac tcagtgatct ctgtgtctct gtggttcagg
     1021 ttttgtgatc gccaggggct caaccgaggt tgggccgagc cccatcgatc gaagacgagt
     1081 atcggctggc agcgactacc gccaataccc tcgcacccac acccagtccc gatgcgcacg
     1141 gatgtagtgg cccggaatcc agaccgcacc gcgatacggc gccacgccga tcacttcgac
     1201 acgtggaaca ggggcgacga ggacggctcg cgtcgcgcag cccgaggcga gcgttgcacc
     1261 cgccagcaac acaactgcga tgatcttctt catggtttgc tccttccgtt tactgcctga
     1321 ttgactgctg gagaaaagac gcatgaccag gcggcatcgt tgacgcatta acatctgtgt
     1381 aacgtgacct cgctcgtcca ctacgtcctg ctctacggcg tgccgctgat cttcatcaac
     1441 gtgctgctcg aacaattggg tgctccggtg cctgcggtgc cggcactcat cgtggcgggt
     1501 gcgttgtcgc gcgacgggaa gttgtcgtcg acgcacgttc ttctggccgc gctcttcgct
     1561 tctctcatcg ccgactacat ctggttcctc cttggtcgcc ggttcggcta ccgaatcctg
     1621 cggacgcttt gccgcatctc gctttcgcct gactcgtgcg tacgcgacac ggaggcgagg
     1681 tttgagagat ggggattgaa gtcgctcctc atcgccaagt tcattcctgg gttctcaacg
     1741 gttgcgccgc cactcgcggg agcgtcgggg cggagcacgc tggcgttcgt gatctacgac
     1801 ggcatcggcg cgttgatctg ggcaggcacc gcagtcgcgg ccggccgtgc gtttcatcgc
     1861 gccatcgatc gcgtgctcct cgctctcgag aacctcggat ggtgggcgat catcatcctt
     1921 ggttcgctgc tcgcgctcgt gatcgtggtc aagtgggcgc agcgcatcca attcatcaag
     1981 caacttcgca cggcgcgcat tcagccgcag gaattgaaag cgatgctcga ccgcggcgag
     2041 ctcccggtgg tgctcgacgt ccgcacgccc ggcgctcgca aacacgatcc acgctccatt
     2101 cccaccgcga tcctggcgct cctcagcgac atcgacgcac aacttggcga tctggcaccc
     2161 catcaggagg tggtgctgta ctgcacgtga ccaaacgaag cctcagccgc ccgtgtggcg
     2221 cgcatgctca tcgacaaggg atacacgaag gtccggccgc tggcgggagg cctcgacgga
     2281 tggatcgacg ccggctttcc ggtggagaat ctggtctgaa tgagtgagtg ttgatcagag
     2341 attgggagat cagagattga gagattagag atcagaggga ttggtgttca atcgagacac
     2401 tttcttcctc gaatccacac tttcctggtc cattgccccc gatccgtcct cttgggtctc
     2461 ctgatctctg atcactaatc cactgatctc tgctccccgg cacgccccgt gcagttttcc
     2521 ggctctgcaa tgaccatcac tgaaaccgcg cgggacacgt ttcttgcccc ggaaaccagg
     2581 gatttctaca tcgagtcgat gcggctcatg cgcgacggcg gcgtgccgtt tctcgtcggt
     2641 ggtgcgtatg cgttcgcgcg gtacacgggc atcgagcgtc acaccaagga tttcgacgtc
     2701 tttctccggc gcgacgattt tccgaaggcg aagagagtct tcgaagcggc cgggtacgag
     2761 tcggagctca actttccgca ctggctggga aaggccatca agggtgacga tttcgtcgat
     2821 ctgattttca gcgccggtaa cggcgttgct gtcgtcgacg agctctggtt cgacttcgcg
     2881 gtcgacgacg aggtattcgg tgtcgacgtc aagctgattc ctgccgaaga gatgatctgg
     2941 tcgaagggct tgatcatgga gcgggagcgc ttcgatggcg cggacgtcgc tcacgttctc
     3001 ctcgccaccg gcgaccggct cgactggcgc cgtctgatcg atcgttacgg aaagcactgg
     3061 cgcgcgctgt atgcccatct cgttctattc gggttcagct atccgtcgga gcgctcgcgg
     3121 gttccggcgt gggtgatgac ggaactgaat gcacgcatcg cggacgaaac caaccaaccg
     3181 gacgcacccg agaaggtctg ttacggcacg atcctctcgc gccagcaata ccttgctgac
     3241 atcaacgagt ggggttacca ggacggacgg ctgcggccga tcggcaacat gacggcggag
     3301 gagatcgccc agtggaccgc cggaatcgaa gtggatggca gcaaatgaag gaaacgctgc
     3361 gcatcgccgc ggtcgccgac attcacgtca agaagacggg acagggcagc atgcaatcgc
     3421 tctttgccgc agcgagcgag caggccgacg tgctgcttct ttgcggcgac ctgacggatt
     3481 acgggctggt cgaggaggca aaggtcctgg cgagagagat caccgcctcc ctgcggattc
     3541 cggtcatcgg catcctcggg aatcacgact ttgaatcagg aaaacaggat gacgtcgtga
     3601 ggatcatgct cgacgccggc gtgacgatgc tcgacggcga ttcgcacgag atcgaaggcg
     3661 ttggtttcgc cggtgtcaaa ggcttcggtg gcggcttcgg caggcggcag ctcggcgcct
     3721 ggggcgagga catcatcaag aagttcgtcc acgaaacgat tgaagaggct ctcaaactcg
     3781 agcaggccct ggccaagctg cgaacgccgc agaagatcgc tgtgattcac tactcgccga
     3841 tccacgccac cgtcgttggc gagcccgagg agatctatcc atttctcgga tcgtcgcgtc
     3901 tggaagaccc gatcgaccgg tatcgctgca atgccgtctt tcacggccac gcccaccgcg
     3961 ggtttctgga agggcgcacg aaaggcaaca tccct
//
LOCUS       DATLVW010000702         6632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_565633, whole genome shotgun sequence.
ACCESSION   DATLVW010000702 DATLVW010000000
VERSION     DATLVW010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565633"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VNN25_20335"
     CDS             <1..836
                     /locus_tag="VNN25_20335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXH93939.1"
                     /translation="RSPDSAPTPDPRRLTPSFYGLDRNLGRAQPDPNVTYVTSLAEIP
                     PAEARLIISNELFDALPFGRVVQRGEELHELWVTEHDGVLDWTEHEADARYDDYFAER
                     GIELDDGQFADVSLEWSPMYDDLCRFVPRGLIVTFDYGLPQTKLFRGRMRRFGTAAAY
                     SQHRVSRDLLINPGQQDLTAHINFDDLRLTGERQHFTTRFFDIQAKFLLALGAAEHEL
                     LQPIGEAAVGSAEEGIVLLDARDDARRLILPDGIGAEIRVLVQERGMGQDRWRFQTKL
                     F"
     gene            1277..1636
                     /locus_tag="VNN25_20340"
     CDS             1277..1636
                     /locus_tag="VNN25_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXH93940.1"
                     /translation="MANYSVLLVAALIVISLPVLTLFIITPLVRAAGGGKVKLEPYEC
                     GIPATSEAIDHRFSVRYFLIAVLFIVFDVETVFLFPWAIRFKQLGWFGAIEMAVFLAI
                     LIVGYVYAWKRKVLSWV"
     gene            1627..2361
                     /locus_tag="VNN25_20345"
     CDS             1627..2361
                     /locus_tag="VNN25_20345"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXH93941.1"
                     /translation="MGLIENRFDPNVLTTSVDKFFNWARKSSLWPMQFGLACCAIEMM
                     AVLDPRHDMARFGAEVFRATPRQADLMIVSGTVTEKMAPIVRRLWDQMPDPKWVIAMG
                     SCATCGGPYDTYAVTQGVDRLIPVDVYIPGCPPRPEALIWGLMELQKKIEKMHVIRKG
                     GESYDFARAAVRDSLRPVHEGPALRAEQAKAQLKQAPISGIKRTPIEEKKSKAAAAPA
                     ATPAASEATEAAPTLSDTPEADEIEN"
     gene            2371..3375
                     /locus_tag="VNN25_20350"
     CDS             2371..3375
                     /locus_tag="VNN25_20350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXH93942.1"
                     /translation="MTDVPEQPSATAPKPAAPAKPVGRSIDEIKRDPLVARACERAGD
                     AIISAKEFAGEISLTVDRERIVEVAKAFRDDGFNYLVDLAGVDYSSYPSWTGDRFGVS
                     YMLYSFSKNNRVRLKVTTDENTPIPSVTSVWKTANWHERETFDMLGLIFEGHPNLERI
                     LMWDGFNGHPLRKDFPIRGIDTGARIYPEVFPEGGGPKAGSTGKDVKDVNIYKGDWPH
                     YGTWPVAEIAARAAEGGMPPAPELAGVVADTAQPTEDAPWHDPEKALPERMELAKAGS
                     LKDKLFAAMAQTDCTACGWDCEGYAAALASGETTDITLCVPGEDETESMLKQLMAGKA
                     "
     gene            3484..4599
                     /locus_tag="VNN25_20355"
     CDS             3484..4599
                     /locus_tag="VNN25_20355"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXH93943.1"
                     /translation="MFDVHEMEVNFGPQHPSTHGVLRVILKIDGERIIDAKPIIGYLH
                     RGTEKLFENMSYPMCIPHTDRMDYVASATNNLGFVETVEKLMGITKQIPKRAQLMRVM
                     LSELQRLSSHLLWLATHAIDIGAMTPFFYCFREREQILDLFEEYCGARLTLNCMRIGG
                     MPDDLPAGWLDDVEAFVDKFDAAIDEYESLLTNNRIWKKRTVGIGVISPEEAIDWGIT
                     GPPLRGSGVEWDLRRAQPYECYPELQFEIPIGKNGDTYDRYLVRMEEMRQSNWILKQC
                     IPLVKATPAGDIKAKISKVVRPPEGEVYHSIESPKGELGYYIIADGKTQMAYRCHVRP
                     PSFVNLQALPAMAKGHLVSDLVALIGTIDIVLGEVDR"
     gene            4697..5902
                     /gene="nuoH"
                     /locus_tag="VNN25_20360"
     CDS             4697..5902
                     /gene="nuoH"
                     /locus_tag="VNN25_20360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXH93944.1"
                     /translation="MTQDLLMRYLIWPLVKFLVAFIIVQVIVAAMNYIERRLLGLMQA
                     RLGPNRVGNEVFEKITPRSLHRFIPGGLLQIIADPIKFILKEDIVPSSSEKVAYFLGP
                     MLPLIPAFLVFCLIPYGPPPTFVVTHVNVGLLLILALTSTGVYGIILGGWASNNKYSL
                     MGGLRSAAQMVSYEVPFGLSIVGVLMLCGSFDLVRIINYQADHIWNLFPQLIAAFIFL
                     VAMNAENNRTPFDLPEAESELVAGYHTEYSAMKFAFFMLAEYSSMIVNCCLMVSLFMG
                     GWTLAINGYGLTTGVLRGWGLAGGILGAVIFISKVMLFMLMYIWFRATFPRFRFDQLM
                     DLGWKWMIPLALANIAVTAVVVLLGLQFNFQGEVVLSIAGAAIIAFTLFLLLRPRAKV
                     IHARRVSEA"
     gene            5877..6404
                     /locus_tag="VNN25_20365"
     CDS             5877..6404
                     /locus_tag="VNN25_20365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HXH93945.1"
                     /translation="MHVEYQKPRWTDKFLLLDMLEGLKTTLRELFKPKVTIRYPEERL
                     EPANRFRGMFKFSFDRCIACKLCALACPIDIIYIDVHDEQREIDGKMKKLKVLDRYDI
                     DVKRCMFCALCEEACPTKPKSIWLTTKTYELASYDRKDLYFNMQQLEYWEGRPEYAGL
                     PADEPEPKQIEGGMP"
     gene            6401..>6632
                     /locus_tag="VNN25_20370"
     CDS             6401..>6632
                     /locus_tag="VNN25_20370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXH93946.1"
                     /translation="MSPALVVFLVIGTIAVASSILVVAMRNPVHSALFLLLTFLCVAI
                     LFVMKGAEFVGAVQVLVYAGGIMVLFLFVVMLI"
BASE COUNT         1220 a         2072 c         2045 g         1295 t
ORIGIN      
        1 gccgatcacc agattctgct ccgacccccg acccccgacg cctgactccc tcgttctacg
       61 gcctcgaccg caatctcggc cgggcccaac cagatccgaa tgtcacttac gtcacgtcgc
      121 tcgcggaaat cccgcccgcc gaggcgcggc tcatcattag taatgagctg tttgatgcgc
      181 tgccgttcgg gcgagtcgtt cagagagggg aagaactgca cgagctgtgg gtcacggagc
      241 acgacggtgt cctcgactgg accgagcatg aagccgatgc gcgatacgac gactacttcg
      301 cggaacgcgg catcgagctg gatgatgggc agttcgcgga cgtttcgctc gaatggtccc
      361 cgatgtacga cgacctttgc cggtttgtgc cccgaggtct gatcgtcacc tttgattatg
      421 ggctgccgca gacgaagctc tttcgcggac ggatgcggcg cttcggaacg gcagcggcgt
      481 actcgcagca tcgcgtcagc cgcgacctcc tgatcaaccc cggccagcag gatctgacgg
      541 cgcatatcaa cttcgacgac ctccgcctca ccggcgagcg gcagcacttc accacccgct
      601 tcttcgatat ccaggcgaag tttctcctcg ccctcggggc ggcggagcac gagttgctgc
      661 agccgattgg ggaggccgct gtcgggagcg ccgaggaagg aatcgtgctg ctggatgcgc
      721 gcgacgacgc caggcggctc attcttccgg acggcatcgg agccgagatt cgcgtcctgg
      781 tgcaggagcg ggggatgggt caggaccggt ggcgttttca gacgaaattg ttctgagtca
      841 aggggttggg gagtgggagg ctggggcgta gccggtgtag cccgcatttc tcacactttt
      901 cagcgactcc ttgtccaagg cgcggtgatg gcggcgagac gccgccaagc cagccggcgg
      961 ggacgccagc gttccatccg cctatggagc gctttgggcc ggcggaacgc cggcgtctcg
     1021 ccggctggcc aggcggcgtc ccgccgccta ccgccggttt ccaggcactt gcgttgaaac
     1081 acgctgccgg caattccctg caagtgtgag aaatgcaggg taggagggga agcacgcggc
     1141 gtgtgccgat ccagcattca tgcgggttct cgtcgctccc acaccccaca gcccactccc
     1201 cactccccaa ggctcaacga atcattgact cctgtcagtc gccgtttaca ataccgcggg
     1261 ctttcgacag gaaaaaatgg cgaactattc agtactgctg gtggctgctc tcatcgttat
     1321 ctcgctcccg gtattgacgt tgttcatcat cacgccgctc gtccgggccg ctggcggcgg
     1381 caaggtcaag ctggagccgt atgagtgcgg gattccggcg acctccgagg cgatcgatca
     1441 ccgcttctcg gtccgctatt tcctgatcgc cgttctcttc atcgtcttcg acgtcgagac
     1501 cgttttcctc ttcccctggg cgatccgttt caaacagctc ggctggtttg gcgcgatcga
     1561 gatggcagtg ttcctggcga tcctgatcgt cgggtatgtt tacgcgtgga agaggaaggt
     1621 tctttcatgg gtctgatcga aaaccgcttc gatccaaacg tcctcaccac ctctgtcgac
     1681 aagtttttca actgggcgcg caagtcgtcc ctttggccga tgcagtttgg gttggcctgc
     1741 tgcgcgatcg agatgatggc cgttctcgac ccgcgccacg atatggcccg cttcggcgcg
     1801 gaggtctttc gcgccacgcc gcggcaggcg gacctgatga tcgtctccgg caccgtcacc
     1861 gagaagatgg caccgatcgt ccggcgtctc tgggatcaga tgcccgatcc gaagtgggtc
     1921 attgccatgg gctcctgcgc gacgtgcggc ggtccgtatg acacgtatgc cgtcacgcag
     1981 ggcgtcgatc gcctgatccc ggtcgatgtc tacattcccg gttgcccgcc gcgtcccgag
     2041 gcgttgatct gggggctgat ggagctgcag aagaagatcg agaagatgca cgtgatccgc
     2101 aaaggcggcg agtcgtacga cttcgcgcgc gcagcggtgc gcgactcgct gcgcccggta
     2161 cacgaaggtc cggcattgcg cgcggaacag gcgaaggcgc aactgaagca ggcgccgatc
     2221 tccggcatca agcggacgcc gatcgaagag aagaagtcga aggctgctgc ggcgcccgcg
     2281 gccacgccgg ctgcttccga agcgaccgag gccgccccaa cactcagcga cactcccgaa
     2341 gccgacgaga tcgagaactg attcccagac atgactgacg ttcctgaaca gccatctgcg
     2401 actgcgccta aaccggccgc tcctgcgaaa ccggtagggc gcagcatcga cgagatcaag
     2461 cgcgatccgc tcgtggcacg cgcctgcgag cgtgcgggcg atgccatcat ttccgcgaag
     2521 gagttcgcgg gcgagatctc gctgaccgtc gaccgcgagc gcatcgtcga ggtggccaag
     2581 gcctttcgcg atgatgggtt caactacctc gtcgatctcg ccggcgtcga ctattcgagt
     2641 tatccgagct ggacgggcga ccgcttcggc gtgtcgtaca tgctctactc gttctcgaag
     2701 aacaatcgcg tccggctgaa ggtcacgacc gacgagaaca ctcccatccc ctccgtcacc
     2761 tcggtatgga agaccgcgaa ctggcacgag cgcgagacgt tcgacatgct cggcctcatc
     2821 ttcgagggcc acccgaatct cgagcgcatc ctgatgtggg atggattcaa cggccatccg
     2881 ttgcgcaagg actttcccat tcgcggcatc gacaccggcg cgcgcatcta tcccgaggtt
     2941 tttccggagg gcggcggacc aaaggcggga tcgaccggca aggatgtgaa ggacgtcaac
     3001 atctacaaag gcgactggcc gcactacggc acctggcctg tcgcggagat tgccgcccgc
     3061 gccgcggaag gtgggatgcc gccggcgcca gagctggcgg gcgtcgtcgc cgacacggcg
     3121 caaccgaccg aggacgcacc gtggcacgat ccggaaaagg cgttgccgga gcggatggag
     3181 ctggccaagg ccggcagcct gaaagacaag ctcttcgccg cgatggcgca gacggattgc
     3241 accgcctgcg gctgggattg cgaaggctac gcagccgcac tggcgtccgg cgaaacgacg
     3301 gacattactc tgtgcgtgcc cggcgaggac gagacggaat cgatgctgaa gcagttgatg
     3361 gcggggaagg catgatgatc gcgggagtcg tttgtcggga gtcgggggtc ggagctaacc
     3421 gatgttctgt ctctctggtt ctccgacccc ccacccccga caccccaccc ccgagggtct
     3481 gccatgtttg acgtacacga aatggaagtc aacttcggtc cccagcaccc gtcgacgcac
     3541 ggcgtgctgc gggtgattct gaagatcgat ggcgagcgga tcatcgacgc caagccgatc
     3601 atcggctatc tccatcgtgg gaccgagaag ctcttcgaga atatgtcgta tccgatgtgc
     3661 atcccgcaca cggaccgcat ggattacgtc gcctccgcca ccaacaacct cggcttcgtc
     3721 gagacggtcg agaagctgat ggggatcacg aagcagatcc cgaagcgcgc ccagctcatg
     3781 cgcgtcatgc tcagcgagtt gcagcggctc tcctcgcatc tgctctggct ggccacgcac
     3841 gccatcgaca tcggcgcgat gacaccgttc ttctattgct tccgcgaacg cgagcagatc
     3901 ctcgacctgt tcgaggagta ttgcggcgca cggttgacgc tgaattgcat gcgtatcggc
     3961 gggatgcccg acgatctgcc ggccggctgg ctcgacgatg tcgaagcatt cgtcgacaag
     4021 ttcgacgcgg cgatcgacga atacgaatcg ctgctgacca acaaccgcat ctggaagaag
     4081 cgcaccgtcg gcatcggcgt catctcaccg gaagaagcca tcgactgggg catcaccggg
     4141 ccgccgttgc gcggatcggg cgtggagtgg gatctccgcc gggcgcagcc gtacgagtgc
     4201 tatccggagc tgcagttcga gatcccgatc ggcaagaacg gcgacaccta cgaccgctac
     4261 ctcgtccgga tggaagagat gcggcagtcg aactggatcc tcaaacagtg catcccgctc
     4321 gtgaaagcca cgccggccgg cgacatcaaa gcgaagatat cgaaagtggt ccggcctccc
     4381 gagggcgagg tgtatcactc gatcgaatcg ccgaaagggg agctgggcta ctacatcatc
     4441 gccgacggca aaacgcagat ggcgtaccgc tgccatgtgc ggccgccgtc gttcgtgaac
     4501 ctgcaggcgc tgccggcgat ggcgaagggt cacctcgtct cggatctggt ggcgttgatc
     4561 gggacgattg atatcgtgtt aggtgaggtc gatcgatgat tcaaaaagcg tttcaccaca
     4621 gagacacaga ggacacagag aagaacctct gtgctctctg tgtctctgtg gttcatcgtt
     4681 cttccggagg tgcgcgatga cccaggacct cctcatgcgc tacctcatct ggccgctggt
     4741 caagttcctg gtcgccttca tcatcgtgca ggtgatcgtg gcggcgatga actacatcga
     4801 gcgccgtctg ctcgggttga tgcaggcgcg gctcggaccg aatcgcgtcg gcaatgaagt
     4861 cttcgagaag atcacgccgc gcagcctgca ccgcttcatt ccgggcggac tgctgcagat
     4921 catcgccgac ccgatcaagt tcatcctcaa ggaagacatc gtcccgtcga gctccgagaa
     4981 agtggcttac ttcctcgggc cgatgctgcc cttgatcccg gcgtttctcg tcttctgcct
     5041 gatcccgtac ggcccgccgc caaccttcgt cgtgacgcat gtgaacgtcg gcctgctgct
     5101 gatcctggcg ctgacctcga ccggcgtgta cggcatcatc ctcggcggct gggcgtcgaa
     5161 caacaaatac tcgctcatgg gcggcttgcg cagcgccgcg cagatggtgt cgtacgaagt
     5221 gcccttcggc ctctcgatcg tcggcgtgct gatgttgtgc ggcagcttcg acctcgtccg
     5281 catcatcaac taccaggcgg atcacatctg gaatctcttc ccgcaactca tcgccgcctt
     5341 tatcttcctc gtggcgatga acgcggagaa taaccgcacg ccgttcgacc tgccggaagc
     5401 ggagtccgag ctcgtggccg gctatcacac cgagtactcg gcgatgaagt tcgccttctt
     5461 catgctcgcc gaatactcgt cgatgatcgt caactgctgc ctcatggtca gtctcttcat
     5521 gggcggctgg acgctggcga tcaacggcta cggcctgacg accggcgttc tgcgcggctg
     5581 gggcctggcc ggcggcatcc tcggcgcggt gatcttcatc agcaaggtaa tgctcttcat
     5641 gctgatgtac atctggttcc gggccacgtt cccccgcttc cgcttcgatc agctcatgga
     5701 cctcgggtgg aagtggatga ttccgctggc actggcgaac atcgccgtga ccgcggtcgt
     5761 cgtgctcctc ggcctacagt tcaatttcca gggagaggtc gtcctctcga tcgccggagc
     5821 ggcgatcatt gcgttcacgt tgttcctgct gctccggccg cgcgcaaagg tgatccatgc
     5881 acgtcgagta tcagaagcct agatggaccg ataagtttct gctcctcgac atgctcgagg
     5941 ggttgaagac gactcttcgc gagttgttca agccgaaggt gacgattcgt tatccggaag
     6001 agcggctcga gccggcgaat cgcttccgag gaatgttcaa gttctcgttc gaccgctgca
     6061 tcgcctgcaa actctgcgcg ctggcctgcc ccatcgacat catctacatc gacgtccacg
     6121 acgaacagcg tgagatcgac gggaagatga agaagctcaa agtcctcgac cgttacgaca
     6181 tcgacgtcaa gcgctgcatg ttctgcgcgc tctgcgaaga ggcctgcccc accaagccga
     6241 aatcgatctg gctgaccacg aagacgtacg aactggccag ctacgaccgc aaggatctct
     6301 acttcaacat gcaacagctc gagtattggg aaggacggcc ggagtacgcc ggtctcccag
     6361 ccgacgagcc ggaaccgaaa cagatcgagg gagggatgcc ttgagtcctg ccctggtcgt
     6421 ttttctggtc atcggcacga tcgcggtcgc ctcgtcgatc ctcgtcgtgg cgatgcggaa
     6481 cccggttcac tcggcgctct tcctgctgct gacgtttctc tgcgtggcca tcctcttcgt
     6541 gatgaaggga gcggagttcg tcggcgccgt gcaggtgctc gtctacgccg gcggcatcat
     6601 ggtgctcttt ctcttcgtcg tgatgctgat ca
//
LOCUS       DATLVW010000703        16454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_482868, whole genome shotgun sequence.
ACCESSION   DATLVW010000703 DATLVW010000000
VERSION     DATLVW010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16454
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482868"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1397)
                     /locus_tag="VNN25_20375"
     CDS             complement(396..1397)
                     /locus_tag="VNN25_20375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXH93947.1"
                     /translation="MKRALMTVAMMALAACASRQHGSPQSAPASTTAVAPQEMTAHGV
                     AGWVGQEFAGRTTANGEIFDPMLMTAAHRTLPFGTLVDVHNPKTGQTVRVRVNDRGPY
                     VGDRLIDLSYAAAQQIGLADGGGGDVDISVVKVGRGDREPPAAFSVNVPDNTSVAAVP
                     PAAATTPAPAPAPTPAPAESQAPPVPATVESVQVTEQHTSGTETRKEVAADGTTIVEV
                     PVTSSPLPAPVPTPAPVTPTKRTTTPATSTPAPAASRPQSATTRTGRYQVQVGAFSQE
                     ANAKALQKKLKVIGQDAMIEHTSLYLVRIGPFETREQAVNERAKLEAAGISAIIITQ"
     gene            complement(1394..2830)
                     /gene="purB"
                     /locus_tag="VNN25_20380"
     CDS             complement(1394..2830)
                     /gene="purB"
                     /locus_tag="VNN25_20380"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXH93948.1"
                     /translation="MTRDAAQNPLTDRYASKEMSYLFSADYKFRTWRRLWIALAESER
                     ELGLAFTDAQIAELKTHANDINYADAERREREIRHDVMSHIYAYGLQAPSAKGIIHLG
                     ATSAFVTDNTDILQMSEALAIIRRRIVNVMAKLRDFAWCWRATPVLAFTHFQPAQLTT
                     VGKRATLWLHDLLLDYREVEFRSANLALLGVKGATGTQASFLSLFDGDASKVNRLEQL
                     VAEKVGMARSYPVSGQTYSRKVDSQVVNVLSGFAQSASKFGYDMRLLQHLREIEEPFE
                     DDQVGSSAMAYKRNPMRAERIDALARHLIVNVLNPAFTAATQWFERTLDDSANRRIAI
                     PEAFLAADGIALIYDNIAAGLVVHEEVIAANVRRELPFMMTENLMMEGVRRGGDRQVL
                     HERVRIHSQAAAGRLKSGASDNDLLDRIAADDFFGIDRASIEEMARPDAFTGLSTQQT
                     EKFLIEDVDPILDAERTQIRSDVGEVRV"
     gene            2890..3210
                     /locus_tag="VNN25_20385"
     CDS             2890..3210
                     /locus_tag="VNN25_20385"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXH93949.1"
                     /translation="MHPAVSTRAPRRAAQTVRRIAVVLVAVIAFGGVASLFFTGRATS
                     ELQQSRKRIESLNAEVAQLEKENARLRAEIDSVKKSTYAVERVAREDLGMSRKGEVIY
                     LLAR"
     gene            complement(3291..5555)
                     /locus_tag="VNN25_20390"
     CDS             complement(3291..5555)
                     /locus_tag="VNN25_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HXH93950.1"
                     /translation="MRRTVFALAVFLATTLVAQTPATDAPMLGFSAGSAAKERTLETQ
                     FDSSLKRDELRDWMKRLSARPHHLGSAYDHDNQLFIASLFKSWGYDTKIEEFSVLFPT
                     PKRRVVELIAPERFVAKLAEPPVPGDATSGQTSEQLPTYNAYSIDGDVTGDLVYANYG
                     IPADYELLERNGVDVKGKIVITRYGGSWRGIKPKVAAEHGAIGCIIYSDPRDDGYAQG
                     DVYPKGAYRNENGAQRGSVMDMPVHPGDPLTPGIGATADAKRIDRKDAEVITKIPVLP
                     ISYSDALPLLRALGGPVAPSDWRGALPITYHLGPGPSRVHLALEFNWNMVPAYDVIAR
                     LAGSQYPDEWVMRGNHSDAWVNGASDPISGLVAELEEAKAIGELAKTGWRPKRTIQFS
                     AWDGEEEGLIGSTEWAETHADELRRNGVVYINSDTNARGFLDAGGSHTLERLVTQVAR
                     DVPDPERNVSVLTRMRAHEAMANMNAESKNAEPKKEAHEHELIRLDALGSGSDYTPFL
                     QHTGVAALNIGYGGEGGGGSYHSVYDSFDHYTRFGDPTFDYGVTQAKTTGRMVLRLAN
                     ADVLPFELSTFADTMSRYLEELTKLAEKTRQDVEERNRFVHERTYEIAADPTKPFVAP
                     KAEAAGIYLNFAPLQNAVLRLKESARAFDRAAAALPADATARQAAADQALMHIEQSLL
                     RADGLPRRPWFRHQIYAPGFYTGYGVKTVPGVREAIEQKEWTEANRQIEIAAGVINAY
                     VAQTEKNLEALRGK"
     gene            5710..6225
                     /locus_tag="VNN25_20395"
     CDS             5710..6225
                     /locus_tag="VNN25_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93951.1"
                     /translation="MSIVFVAGNAADVFAPDLAQTIDAELRRRFPTLPFVEAEAYRSD
                     PVEAIGWSKLQESVMRAIPSAPQITGIDAYQSVYLPLPFASVEHILIPTVADPLEAGS
                     LPVLLDELRLFATSVSLPTDDVELMQLAAKYLEDDALFDADLDVQTYVQLFLTARQAA
                     AHGAALWIALE"
     gene            6269..8818
                     /gene="mprF"
                     /locus_tag="VNN25_20400"
     CDS             6269..8818
                     /gene="mprF"
                     /locus_tag="VNN25_20400"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HXH93952.1"
                     /translation="MDRLKRLVPFLKPLLVLALFAIALRVLHDTLSGYRYRQVLAYIN
                     TLPRQQLVLAVVFTLLSYLVMTGYDTLAFRYIRHPLPYFKIALASFIGYAFNNSVGFS
                     GLVGGSLRFRLYNAWRLTAVQIAKVIAFCTITFWLGFVLLGGIFFLATPPAIPPSIHL
                     PFVSIRLLGIALLLPAIGYLLWIVIRREPIRIKQWEFELPSLELFLAQVTTSCLDWIL
                     AASVLYILLPASLPLTFSQFLCIFLLAQIAGVASSVPGGLGIFEAIIIVFLVPFFSAS
                     AILGALVAFRAIYYLLPLLVAIVLLAIHEIVEKREGVAKAWKIFGRWAPGLAPQLLAF
                     TTFVGGAVLLFSGATPTLPGRLGFLRRVVPLPIVELSHFFGSITGAALLLLARGLQRR
                     LDGAYQVTLGVLGAGILFQLFKGGDFEEAIILAVMLFALVASRRHFYRKAALLNEGFG
                     PGWITAIILVVITSAWLGFFSYKHVEYSRDLWWHFQFRADAPRFLRASVGVIAVLLLF
                     SIQRLLRPAPEEPDPPRIEEVEHAAAIIVNEKNSQTNLALLGDKPLLFSPSGQAFIMY
                     GIEGRSWIAMGDPVGAEDEKQELIWQFRELCDLHAGWPVFYEVQRQNMHLYLDLGLTL
                     LKIGEQGRVGLEEFSLEGRHRKWMRYVLRRVEADGCRFEIIDPGPVVEELRAISDSWL
                     AEKRTREKGFSLGFFSEEYIRRCKVAVVRRGDRIVAFSNLWEGAGVELSGDLMRHLPD
                     APNGVMDYMFVSLILWAKEHGYHWFNLGMAPLSGLESRSLGTIWNRMGALAYRFGENF
                     YNFQGLRQYKEKFDPVWEPMYLASPGGFVLPRILTNLAALISGGLRGVVSK"
     gene            8845..10554
                     /locus_tag="VNN25_20405"
     CDS             8845..10554
                     /locus_tag="VNN25_20405"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXH93953.1"
                     /translation="MKSAATSALLLFAIVSTTLAAGKTSKPAPKPFSVVEASISDMQA
                     ALKEGRVTSHELVQQYLIRIATYEDKLHAAITVNPNALKEADERDRERAAGKIRGPLH
                     GIPIALKDNIQTTDLLTTGGALAFANLTPPYDATLTKNLRDAGAIIMAKTQLTELANW
                     VAGNPPMPTNYNALNGYGFNPYDPRRDPRTETGDGRPALATGGSSSGAGTTANFWAAN
                     VGSETSGSILSPSNQNMLAAIKPTVGRISRYGVIPITADQDTAGPMAKFVTDAAILFG
                     VLEGNNPDANDPATTRCTPPPNHDYTQFLKKDGLKGARIGIPRSGFYDKITLPGATTP
                     RGGLNPDQTKAMNDAIAVLKEQGAVVVDPADIPSITDKNAADNYFLWGVCTDMDEVKG
                     KKCSIALAYGMERDFNKWLAALGKDAPVKTLADLRKWNTDHAKMGAIKYGQSLLDISD
                     AMDLDLFKSRYESDRANDIRLTATHGIDEVMKSQNLDAILFPGPSGASIAARPGYPTV
                     IVPWAMVPNAPTTAFPETFKALPQPYGVSFTGMACSEPTLLKLAYSFEQATKRRVPPP
                     STP"
     gene            complement(10563..11885)
                     /locus_tag="VNN25_20410"
     CDS             complement(10563..11885)
                     /locus_tag="VNN25_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93954.1"
                     /translation="MRSTRLLFALTTLLASHAAIAQFSQQGPKLVGADAAGAAAQGEA
                     VALSGDGNTAIVGGHLDSSGFGAAWIWTRNGGVWTQQGSKLVGAGGVIGPGYLAVDQG
                     RAVALSADGNTAIVGGCYDNDGVGAAWIWTRSQGIWSQQGPKLVGSGYVPNFAASGCC
                     DGVPTADVFQGWTVALSGDGNTAIVGGPGDNGRAGAVWIWTRSGGVWTQQGPKLVGSG
                     SVKVGIQTAQQGSSVAISGDGNTALVAGIGAGERVGALWVWTRSGGVWSQQGARLVAS
                     DPVESGYQGTSAALSADGNTAMTRAFSGGVWVWTRSGDVWTQQGTKLVGTGASESAIQ
                     GWSVALSGDGNTAMIGGPFDSGDNTGSAWLWTRSGGIWRQQGNKLFGSGADGKTQFGA
                     SVSLSADGKTAIVGGSASLYTLKTGIGAAWVFVSEEPVPSNRRRGINH"
     gene            complement(12570..14618)
                     /locus_tag="VNN25_20415"
     CDS             complement(12570..14618)
                     /locus_tag="VNN25_20415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH93955.1"
                     /translation="MLPVQQKPEPPKSRRGARLFLVWLLGIGIIIAGELITRFVLRPL
                     NLPGGAFFGFTTFLACLLVIALTLYGLAVFVRWTLRKLFWRVGRRLFLSYTLIGVLPF
                     FLFAILLLTIGYMICGVMTHAALRGERQASLGQLESSAFEYSLTGKHPSGATKALEIY
                     DTANASGNKLPSWLKSTTFSGMVWRDGQALLVASRQASQQEGAPVRTIVFVEPLDAEW
                     LEQFQDKSGMTILTKVGDRTTARYRRGVRVEKGDGGLNFDGEDDSQFTDLFMRSVGRK
                     VIWPDLTDLTDWNGGPTAEGKHTIFTMIVNPFQNLFHFYFGPEADNYVSRLLQFMSVL
                     VVLLLVFYAMAVLFAAVLIFSITRAVNRIEKGTKAVERGDFTYRIAMKPHNQLGEMAQ
                     SFDRMTESIAALLVNVAENERLQSEIEIAASIQRNLLPKEGPQFRGVSFSAHFEPTAS
                     IGGDYYDVFNIDKTRLAVAIGDVSGHGLSTGLVMAMVKAAITTLVEEGAEETSLFHRL
                     NDLVFRSTERRAFMTLAFTIFDLEKGTIRHTNAGHLYPYLLREGQRPRGIEVPSLPLG
                     VRSTISTHTAEVDLHEGDAIVYLSDGIVEAQDENGEPFGFDHLEALLTESTDRSPSTI
                     RDRILEAVARHCGTRPADDDRTVMILRFDNFKPSRPAAVVAESAVATVLDNEEVVATT
                     "
     gene            complement(14915..15430)
                     /locus_tag="VNN25_20420"
     CDS             complement(14915..15430)
                     /locus_tag="VNN25_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93956.1"
                     /translation="MGETKLFQVCYEGELTIDVSNAMRRLGAEPNFDQSWHVWVPDGG
                     HAELIVRYLRMEVGIEARVLVGCAQFTTSRDFLLIRHSLTPGADYSELHDAIARLGVV
                     VELPFESTFVVQSPDTTDVQTLGEALGELCPDDALMVTGISHDWAYCDGTTSRMNVAE
                     LEPRELQFRTF"
     gene            15539..15748
                     /locus_tag="VNN25_20425"
     CDS             15539..15748
                     /locus_tag="VNN25_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXH93957.1"
                     /translation="MRFNDDEVKGKIDQASGAVKENIGRARGDQALENEGADQRASGK
                     FEAGFGKTRRKMGEAVKDLGDKLGK"
     gene            complement(15812..16105)
                     /locus_tag="VNN25_20430"
     CDS             complement(15812..16105)
                     /locus_tag="VNN25_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93958.1"
                     /translation="MANVRVTLDSATKTISVDDSHVRVSVSGKTKVQWSCREGNFQIK
                     FKPGSDWPNPVTTNTGGVWMAECGPFTNPGRTLEYAVESTGYKTLDPEILIDT"
     gene            complement(16141..>16454)
                     /locus_tag="VNN25_20435"
     CDS             complement(16141..>16454)
                     /locus_tag="VNN25_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93959.1"
                     /translation="DLQLNAADNVTRARLAACLAKRGDLRSATGQISRAISDDPTDIN
                     VMYNAGVVANVAGAKQAAAQYLKRAVDQGYSRIEIERDPEFSILRESAEYKRIFATRV
                     Q"
BASE COUNT         3021 a         5219 c         5023 g         3191 t
ORIGIN      
        1 ggatcaaaac gagccgtcga tggatcaaaa cgtaccgtcg atggccgaaa agaaccatcg
       61 atggatcaaa aagcaccgtc ggtcgttcaa aaagcaccgt cgttggttca aaacgcatca
      121 tcgtgggtgc atgacgcagc attgcgggtg tgaatcgcgg catcgtgggg gcaggacgca
      181 gcgtcgagtg tgtgaacccc cacggccccc ggtccgaccg ctttaccttg tcaccctcac
      241 ggtcgtagat caagggaacc gttcaagagt gtggaaatgc ggggctagct gctagccggc
      301 cgccgcccgc gtcctgatct cttgtctctt gatctctgtc tcaagtcaag gaccggacag
      361 ccacgctttc gctccgactc ccgacccccg cgtatctact gcgtaatgat gatcgccgag
      421 atcccggctg cttccagctt ggcgcgctcg ttgactgcct gctcgcgcgt ttcgaacggg
      481 ccaatacgca cgaggtagag agacgtgtgc tcgatcatcg cgtcctggcc tatgactttc
      541 agtttcttct gcagcgcctt cgcgttcgct tcctgggaga acgcgcctac ctgaacctga
      601 tatcgcccgg tgcgagttgt cgcggactgc ggccgcgacg cggcgggagc gggtgttgat
      661 gtcgcagggg tagtcgtccg tttggttggc gtaacgggag ccggcgttgg caccggagcg
      721 ggaagcgggg atgacgtcac cgggacttcg acgatcgtcg tgccgtcggc ggcgacttcc
      781 tttcgcgtct ccgtgccgct tgtgtgctgc tcagtgactt gcacgctctc gacagttgca
      841 ggaacgggag gcgcctgaga ctctgcaggc gctggagtgg gagcgggagc tggtgcagga
      901 gttgtcgccg ccgcaggtgg aactgccgcc acgctcgtgt tgtcgggcac gttcaccgag
      961 aacgcggcgg gcggctcccg gtcgccgcgg ccgaccttca ctacgctgat gtcgacgtcg
     1021 ccaccgccgc catccgcgag tccgatctgc tgcgctgccg cataagaaag gtcgatgagc
     1081 cggtcaccga cgtacggtcc gcggtcgttg acgcggacgc gcacggtctg gccggtcttc
     1141 ggattgtgaa catctacgag cgttccgaac gggagcgtgc ggtgcgccgc cgtcatcagc
     1201 atcggatcga agatctctcc gttggccgtc gtgcggccgg cgaactcctg gccgacccag
     1261 cccgctacgc cgtgcgcggt catctcctgc ggcgccacgg ctgttgtcga tgccggcgcc
     1321 gattgcggcg atccatgttg ccggcttgcg cacgccgcca acgccatcat cgccacggtc
     1381 atcagagctc gcttcatacc cgcacctcgc caacatccga tctgatctgc gtccgttcgg
     1441 catcgagaat gggatcgacg tcctcgatca ggaacttttc agtctgctgc gtcgaaaggc
     1501 ccgtgaacgc atcgggccgc gccatctcct cgatcgatgc gcgatcgatg ccgaagaagt
     1561 catccgcggc gatgcggtcc agaagatcgt tatcgctcgc gcccgatttc agcctgccgg
     1621 cggcggcttg cgagtgaatc ctgacgcgct cgtgcagcac ctgccgatcg ccgcctcgcc
     1681 gcacgccctc catcatcagg ttctccgtca tcatgaaggg aagctcccgc cggacgttcg
     1741 ccgcgatcac ttcctcgtga acgacgagcc ccgcggcgat gttgtcatag atcagtgcga
     1801 ttccatcggc agcgaggaag gcctccggaa tggcgatacg ccggttggct gaatcatcga
     1861 gcgtgcgctc gaaccattgc gtggcggcgg tgaatgcggg attcaggacg ttgacgatga
     1921 ggtgccgcgc cagcgcatcg atccgttccg cgcgcatcgg attgcgcttg tatgccattg
     1981 ccgaggagcc gacctggtcg tcttcgaacg gctcttcgat ctcgcgcaga tgctgcagaa
     2041 ggcgcatgtc gtagccgaat ttggatgcgg actgcgcgaa gcccgacagc acgttcacca
     2101 cctgcgagtc gaccttgcgc gagtacgtct gaccggagac gggatagctt cgggccatcc
     2161 cgactttctc ggcgacgagc tgctccagcc ggttgacctt gctggcatct ccgtcaaaaa
     2221 ggctgaggaa tgacgcctgc gttccggtcg cgcctttcac gccgaggagc gcgaggttcg
     2281 cgctgcggaa ctcgacttcg cggtaatcga gcagcagatc gtgaagccag agcgtggcgc
     2341 gctttccgac cgtggtgagc tgcgccggct gaaaatgcgt gaaggcgagc acaggcgtgg
     2401 cgcgccagca ccatgcgaaa tcgcgtagct tggccatcac gttcactatg cgccggcgaa
     2461 tgatagccag cgcctcgctc atctggagga tgtcggtgtt gtcggttacg aaggccgagg
     2521 tggcgccgag gtggatgatg cctttcgcgg atggcgcctg gagtccgtag gcgtagatgt
     2581 gcgacatgac gtcgtgccgg atctcgcgct cgcggcgctc ggcgtcggca tagttgatgt
     2641 cgttcgcgtg ggttttcagc tcagcgatct gagcgtcagt aaaggccaac cccagctccc
     2701 gctcggactc ggcaagcgca atccatagcc gccgccacgt ccgaaacttg tagtcggccg
     2761 agaagagata cgacatctcc ttcgaggcat atcgatcggt aagagggttc tgtgcggcgt
     2821 ctcgcgtcac cgaatcgaat tatacggcgt tgacacggcc gcactgaccc gcgacactac
     2881 ctttgacaca tgcatcccgc cgtctccaca cgggctccac gccgggccgc gcagaccgtc
     2941 cgcagaatcg cggtggtcct ggtggctgtc atcgcgtttg gcggcgtggc gtcgctcttc
     3001 ttcaccggtc gcgcaacctc ggagctgcag caatcgcgca agcgcatcga atcgctgaac
     3061 gccgaggtgg cgcagctcga gaaagagaac gcccgccttc gcgcagagat cgacagcgtg
     3121 aagaagtcga cttacgcggt cgagcgcgtc gcgcgcgagg atctggggat gtcgcgcaag
     3181 ggcgaagtga tttacctgct cgcgaggtga gtccaattct ttttgaacca cagagactca
     3241 gaggacactg aggacgccgt gtactctgcg tctctgtggt taaccggttt tcacttcccg
     3301 cgcagcgcct ccaggttctt ctccgtttgg gcgacgtacg cgttgatcac gccggcggcg
     3361 atctcgatct ggcggttggc ttcggtccac tctttctgtt cgatcgcttc acgcacgccc
     3421 ggcacggtct tgacgccgta gccggtgtag aagccggggg cgtagatctg gtggcggaac
     3481 cacggcctcc gcggtaatcc atccgcccgc agcagtgact gctcgatgtg catcaacgcc
     3541 tgatcggcag ctgcctgacg cgcggttgca tctgccggca gcgctgccgc ggcccggtcg
     3601 aacgcacgcg cactctcttt cagacgcaaa accgcattct gcagcggcgc gaaattgagg
     3661 tagattccgg ccgcttccgc cttcggcgcc acgaatggtt tcgtcggatc ggcggcgatc
     3721 tcgtaggtgc gttcgtgcac gaagcggttt cgctcttcga cgtcctggcg cgttttttcg
     3781 gccagcttcg tcagctcctc gaggtagcgg gacatcgtgt cggcgaaggt gctcaactcg
     3841 aagggaagca cgtccgcgtt ggcgagccgc agcaccatcc gccccgtcgt cttcgcctgc
     3901 gttacgccgt aatcgaaggt gggatcgccg aaccgcgtgt aatgatcgaa cgagtcgtac
     3961 accgagtgat acgacccgcc gcccccctcc ccgccgtatc cgatgttcag tgcggcgacg
     4021 ccggtgtgct gcaggaacgg cgtgtagtcg gagccggagc cgagcgcgtc gagccggatc
     4081 agttcatgct cgtgcgcttc tttcttcggt tccgcgttct tcgattccgc attcatgttc
     4141 gccatcgcct cgtgggcgcg catgcgcgtg aggacggaaa cgttgcgctc cggatcagga
     4201 acgtcgcgcg ccacctgcgt gacgaggcgc tcgagcgtgt gcgatccgcc ggcatcgagg
     4261 aagccgcgcg cgttcgtatc ggagttgatg tagaccacgc cgttgcgccg aagctcatcc
     4321 gcgtgcgtct ccgcccattc ggtcgacccg atgagccctt cttcttcacc gtcccacgcg
     4381 gagaactgga tcgtccgctt cggacgccag ccggtcttcg ccagctcgcc gatcgctttt
     4441 gcttcctcca actccgcgac cagaccgctg atcggatcgc tcgcgccgtt cacccaggcg
     4501 tcggagtgat tgccgcgcat gacccactcg tcgggatact gcgatcccgc caggcgcgcg
     4561 atgacgtcgt acgcaggaac catgttccag ttgaattcga gcgcgaggtg cacgcgggat
     4621 ggaccaggcc cgaggtgata ggtgatgggc agcgcgccgc gccaatcgga aggcgcaacc
     4681 ggaccgccga gcgcgcgcag cagcggcagc gcatccgaat aggagatcgg caataccggg
     4741 atcttcgtga tgacctcggc gtcttttcgg tcgatccgct tggcgtccgc ggtggctccg
     4801 atgccgggtg tgagcggatc gccgggatgc acgggcatat ccatcaccga gccgcgttgc
     4861 gcgccgttct cgttgcggta ggcgcccttc ggatagacgt cgccctgcgc gtagccgtcg
     4921 tcgcgaggat cggagtagat gatgcagccg atggcgccat gttcggcggc gaccttcggc
     4981 ttgatgccgc gccacgaacc gccgtagcgg gtgatgacga tcttcccctt cacgtcgacg
     5041 ccgttgcgct cgagcaactc gtagtcggca gggatcccgt aattcgcgta gacgagatcg
     5101 ccggtgacgt caccgtcgat cgagtaggcg ttgtaggtcg gaagttgctc gctcgtctgc
     5161 cccgacgtcg catcgcccgg taccggcggc tcggcgagct tcgccacgaa gcgctcgggc
     5221 gcgatgagct cgacgacgcg ccgcttcgga gtcgggaaaa gcacgctgaa ctcttcgatc
     5281 ttcgtgtcgt agccccacga cttgaacaac gatgcgatga acagctggtt gtcgtgatcg
     5341 taggcggagc cgaggtggtg cggccgtgcc gagaggcgct tcatccaatc gcgaagctcg
     5401 tcgcgtttca gcgacgagtc gaactgcgtc tcgagcgtac gttccttcgc cgcgcttccc
     5461 gcgctgaatc cgagcatcgg cgcatcggtt gcaggtgtct gggcgacgag ggtggtggcg
     5521 agaaagacgg caagggcgaa aacagtgcgg cgcatgggcg gggattgtaa tttggttttt
     5581 tcacgcgaag acgcgaaggc gcgaagaaac ctgctccgcg ttctccgcgt ctccgcgtga
     5641 aatgctttta ttaaatccca gcctcgatcc ccactgcaca acagcgccca cgtacggtac
     5701 gctgcctgtg tgtcgatcgt cttcgtcgcc ggcaacgctg ccgatgtctt tgcaccggat
     5761 ctcgcgcaga ccatcgacgc cgaacttcgg agacgatttc ccactctccc gtttgtcgaa
     5821 gccgaggcct atcgctccga tccggtcgaa gcgattggat ggtcgaaatt gcaggagagc
     5881 gtcatgcgcg cgatcccctc cgcgccgcag atcactggca tcgacgccta ccagtcggtc
     5941 tatctgccgc tgccgttcgc gagcgtcgag cacatcctca tcccgaccgt cgccgatccg
     6001 ctggaagccg gcagcctgcc tgtcctgctc gatgagttgc gcctttttgc cacgagcgtt
     6061 tcgctgccca cagacgacgt cgagctgatg caacttgcag cgaagtacct cgaagatgat
     6121 gcccttttcg acgctgacct cgacgtgcag acgtacgtgc agctctttct gaccgcccgg
     6181 caggcggcgg cacatggcgc ggcgttgtgg attgcgctgg agtgagatca cggcctccca
     6241 tgaccccgac cgcatagaat cgccacgaat ggatcgactc aaacggctcg tgccgtttct
     6301 caaaccgctt ctggtgctgg ctctgttcgc catcgcgctg cgggtactgc acgacacgct
     6361 gtctgggtac cgctatcgcc aggtcctcgc ctacatcaac actctcccgc gccagcaact
     6421 cgttctcgct gtcgtcttca cgctcctcag ctacctggtg atgaccggct acgacacgct
     6481 ggccttccgt tacatccggc atccccttcc gtacttcaag attgctctgg cctcgttcat
     6541 cggctacgcc ttcaacaaca gcgttggctt ctccgggctc gtgggcggct cgttgcgctt
     6601 ccggctttac aacgcctggc gtttgaccgc cgtccagatc gccaaagtca tcgccttctg
     6661 cacgatcacc ttctggctcg gcttcgttct cctcggcggt attttctttc tggccacgcc
     6721 gccggcgatt cccccctcga tccatttgcc gttcgtgtcg atacggctcc tcggcatcgc
     6781 gcttcttctg ccggcgatcg gctacctgct ctggatcgtg atccgccgcg agccgatccg
     6841 catcaaacag tgggagttcg agctgccatc gctggaattg tttctggcgc aggtaacgac
     6901 gtcgtgcctc gattggattc tcgccgccag cgttctctac atcctgctgc cggcctcgct
     6961 gccgctgacg ttcagccagt tcttgtgcat cttcctgctg gcgcagatcg ccggcgtcgc
     7021 ctccagtgtg ccaggcggcc tgggtatttt cgaggcgatc atcatcgtct tcctggtgcc
     7081 attcttctcg gcatcggcga tcctcggcgc gctcgtcgcc tttcgcgcca tctactacct
     7141 tctgccgctc cttgtggcca tcgtgctgct ggcgatccac gagatcgtcg agaagcgcga
     7201 aggagtggca aaagcgtgga agatcttcgg acgatgggcc ccgggcctcg cgccgcagct
     7261 tctcgccttc acgacgttcg tcggcggcgc ggtactgctc ttctccggcg cgacgccgac
     7321 gcttccggga cgtcttgggt ttcttcgccg cgtcgtgccg ctgccgatcg tcgagctgtc
     7381 acacttcttc gggtcgatca ccggcgcggc tttgctgctg ctcgcgcgcg ggctgcaacg
     7441 acgcctcgac ggggcttacc aggtcaccct tggtgtcctc ggtgcgggca tcctgttcca
     7501 gctcttcaaa ggcggcgact tcgaagaggc catcatcttg gccgtgatgt tgttcgcgct
     7561 cgtggcttcc cggcgccact tctaccgcaa ggccgctctc ctcaatgaag gcttcgggcc
     7621 gggatggatc accgccatca tcctcgttgt gattacctcg gcgtggctcg gtttcttcag
     7681 ctacaagcac gtcgagtatt cgcgcgatct ctggtggcac tttcagttcc gcgccgacgc
     7741 gcctcgcttt ctgcgggcct cggtcggcgt catcgcggtg ctgcttctgt tttccatcca
     7801 gcggctactc cgtcccgcac ccgaggagcc cgatccaccg cggatcgagg aggtcgagca
     7861 cgccgccgca atcatcgtca acgagaagaa cagccagacc aacctcgccc tcctcggcga
     7921 caagcccctg ctcttcagcc cgagcggaca agcattcatc atgtatggca tcgaaggccg
     7981 cagttggatt gccatgggcg atccggtcgg tgccgaagac gaaaaacaag agctgatctg
     8041 gcagttccgc gagttgtgcg atctgcacgc aggctggcca gtgttctacg aggtgcagcg
     8101 gcagaacatg cacttgtatc tcgacctcgg gctgacgctt ctgaagatcg gcgagcaagg
     8161 gcgcgtgggg ctggaggagt tctcgctcga agggcggcac cgcaagtgga tgcgctacgt
     8221 gctgcgtcgc gtcgaggcgg acggctgccg tttcgagatc atcgatccgg ggccggtcgt
     8281 cgaagagctg cgtgccatct ccgattcctg gctcgccgag aagcgcacgc gcgagaaggg
     8341 cttttccctc ggattcttct ccgaggagta catccggcgc tgcaaggtcg cggtcgtgcg
     8401 ccgaggcgat cgcatcgtgg cattctcgaa tctgtgggag ggggcaggcg tcgagctttc
     8461 cggtgacctc atgcgccacc tgcccgacgc gccgaacggt gtgatggact acatgttcgt
     8521 aagcctcatt ctctgggcga aagagcacgg ataccattgg ttcaacctgg gcatggcgcc
     8581 gctgtccggc ttggagagcc gctcgctggg caccatctgg aaccgcatgg gagcgctcgc
     8641 ctatcgcttc ggcgaaaact tctacaactt ccagggcctg cgacaataca aggagaagtt
     8701 cgacccagtc tgggagccga tgtaccttgc cagccccgga ggattcgtcc tgcctcggat
     8761 cctcacgaat ctcgccgcgc tcatatcggg aggattgcgc ggagtggtct cgaagtaacc
     8821 actcctaact tacaatcccg cgccatgaag agcgccgcaa catccgccct cctccttttc
     8881 gcaatcgtca gcacgacact cgccgcgggt aaaacaagca agccggcacc gaaaccattc
     8941 agcgtcgtcg aagccagcat ctccgacatg caggctgcgt tgaaagaggg ccgcgtcacc
     9001 tcgcatgagc tcgtgcagca gtatctgatt cgcattgcca cgtacgaaga caagctgcac
     9061 gccgcgatca ctgtcaaccc gaacgcgctc aaagaagccg acgagcgcga tcgcgagcgt
     9121 gccgcaggaa agattcgcgg accgctgcac gggattccca tcgcgctcaa ggacaacatc
     9181 cagactaccg acctgctcac cactggcggt gccctggcgt tcgcgaacct cacgccgccg
     9241 tacgacgcga cactcacgaa gaacctccgc gatgcgggcg cgattatcat ggccaagacg
     9301 cagctcaccg agctcgcgaa ctgggtggcc ggcaatccgc cgatgccgac gaactacaac
     9361 gcgctcaacg gctacggctt caatccctac gatccgcgcc gcgaccctcg caccgaaaca
     9421 ggcgacggcc gacctgccct tgcaacgggc ggctccagtt ccggcgcagg aacgaccgca
     9481 aatttctggg cggcgaacgt cggcagcgaa acgtcaggat cgatcctcag cccgtcgaac
     9541 cagaacatgc tggccgcgat caagccgacc gtcggccgca tctcgcgata cggtgtcatc
     9601 cccatcaccg ccgatcagga cacggcagga ccgatggcca agttcgtcac cgatgcggcg
     9661 attctcttcg gcgttctcga aggcaacaat cccgatgcga acgatccggc cacgactcgt
     9721 tgcacgccgc cgcccaatca tgactacacg cagttcctga agaaggacgg cctcaaagga
     9781 gctcgtatcg gcatcccgcg ctccggcttc tacgacaaga tcacgctccc cggcgcgacc
     9841 actccgcgcg gaggtctgaa ccccgatcag acgaaggcga tgaacgacgc cattgcggtg
     9901 ctcaaagaac agggcgccgt cgtcgtcgat cctgccgaca tccccagcat caccgacaag
     9961 aacgcggctg acaactactt cctctggggc gtatgcacgg acatggacga ggtgaagggc
    10021 aagaagtgct cgatcgcact cgcctacggc atggagcgcg acttcaacaa atggctcgcc
    10081 gcccttggca aggatgcgcc ggtcaagacg ctggccgacc tccgcaagtg gaacaccgat
    10141 cacgccaaga tgggcgcgat caagtacggc cagtcgctgc tcgacatctc cgacgcgatg
    10201 gatctcgatc tcttcaaatc gcgctacgaa tccgaccgcg ccaacgacat ccgcctcacc
    10261 gctacgcacg gcatcgacga ggtgatgaag tcgcagaacc tcgacgccat cctcttcccc
    10321 ggcccgagcg gcgcatcgat cgccgcccgc cccggttacc cgactgtgat cgtgccgtgg
    10381 gcaatggttc cgaatgcgcc gaccacggct ttcccggaaa cattcaaggc cttgccgcag
    10441 ccgtatggcg tcagtttcac cggtatggcc tgcagcgagc cgacgttgct caagctggcg
    10501 tactcattcg agcaggccac gaagaggagg gtgccaccac cgtcgacacc gtaggacgac
    10561 aatcagtgat tgatgccgcg tcgtcggttc gacggtaccg gttcctccga gacgaacacc
    10621 cacgccgctc cgattccggt tttcaatgtg tagaggcttg ctgagcctcc gacaatggct
    10681 gtcttgccgt ctgccgagag ggagacggag gcaccaaatt gcgtttttcc gtcggcgccc
    10741 gagccgaaca gtttgttgcc ctgctgtcgc cagattccgc cactccttgt ccagagccac
    10801 gccgagccgg tgttatcgcc gctgtcgaac ggtccgccga tcatggctgt gttgccgtcg
    10861 ccggagagtg ctaccgacca gccttggatt gcggactccg aggcacccgt gccgaccagc
    10921 ttggtgccct gctgcgtcca gacatctccg ctcctggtcc agacccacac cccaccagaa
    10981 aacgctcggg tcatggcggt gttgccgtcg gctgaaaggg ccgcggacgt gccctgatag
    11041 ccggattcaa cagggtccga ggcaaccagc ctggctcctt gctgggacca gacacctccg
    11101 ctcctggtcc agacccatag ggctccgacg cgctcgccgg cgccgatccc tgcgacaagg
    11161 gccgtgttgc catcgccgga gatggccacg gacgagcctt gttgggcggt ctggatgcct
    11221 accttgacag agcccgagcc aaccagcttg ggcccttgct gggtccagac tccgccgctc
    11281 ctggtccaga tccacacggc accggctcta ccgttgtcgc cggggccacc gacgatggcc
    11341 gtgtttccgt cgccggagag ggcgaccgtc cagccttgaa atacgtctgc agttggaacg
    11401 ccatcgcagc aaccggacgc ggcgaagttc ggcacatacc ctgagcccac cagtttggga
    11461 ccttgctgac tccagattcc ctggctcctg gtccagatcc acgcagcgcc gacgccgtcg
    11521 ttgtcgtagc atccgccgac gatggcagtg ttgccgtcgg cggagagggc cacggcccgt
    11581 ccctggtcaa cggccaggta gccaggtccg atcacgccgc ccgcgcctac cagctttgaa
    11641 ccctgctgtg tccagacccc tccgtttctg gtccagatcc aggcggcccc aaacccactg
    11701 ctatcgaggt gtccgccgac gattgccgtg ttgccgtcgc ccgagagggc tacggcttcg
    11761 ccttgcgccg ccgcccctgc ggcgtccgct cccaccagct tcggtccctg ctgtgagaat
    11821 tgcgcgattg ccgcgtgaga cgcaagcagt gtggtgagag cgaagaggag ccgggtgcta
    11881 cgcatagacg gaattatcac gcatgcagcg ttagtcagac aacccacacc gcttcggatc
    11941 gtgagtcacg ttggacggct tatcgtggtg gccgaagcaa aaggcaacca cagagacaca
    12001 gagagcacgg aggtgtctct gtgacctcgg tgcctctgtg gtaaagcggt tttccaaatt
    12061 acggactcga acacattggt tgggaatttc cggcagagtg tttcaatcga gcgcctgctc
    12121 cccacgctcg agaacagcgt gagaaatgct agttggcggc aaagcattga acagctgaca
    12181 cttgcggctt cgtcgttggt gatcgtcacg agaaggatcg cgactcgccg gcggcgaatc
    12241 gagatctacc aggcttagat cggtatctaa aaaaagagat gcgcatctca aaaaagagat
    12301 ccgcatctcg aaaaagagat ccgcatctaa aaaaagagat cgcgatctcc caagaagaga
    12361 tccgcatctc caaacaagag atccgcatct cgaaacaaga gatccacatc tcctaaacag
    12421 agatccgcat ctcctcggaa gagatgcgca tctcaaaaaa gagatcgcga tctcccaaag
    12481 cgagatccgc atctcccaaa gcgagattcg catctaccaa agggagatcg tgacctgccg
    12541 ggcagggatc gagacctcgc cgatcccgat tacgtcgtcg cgacgacttc ctcgttgtcg
    12601 agaaccgtcg caaccgcgct ttccgcaacc actgctgcgg gccgcgaggg cttgaagttg
    12661 tcgaagcgga ggatcatcac ggtgcgatcg tcgtcggcgg ggcgcgtgcc acagtggcgg
    12721 gcgacggctt cgaggatgcg gtcgcggatc gtgcttgggg aacgatcggt cgactccgtc
    12781 agcagcgctt cgaggtgatc gaaaccgaag ggctcgccgt tctcgtcctg cgcctcgacg
    12841 atgccgtccg acagatagac gattgcgtcg ccttcgtgca ggtcgacctc ggcggtgtgc
    12901 gtgctgatgg tgctgcgaac gccgaggggg agggagggga cctcgatgcc gcggggacgc
    12961 tgcccttcgc ggagaagata cggatagaga tggccggcgt tggtgtggcg aatcgtcccc
    13021 ttctcgagat cgaagatcgt gaaggccagc gtcatgaacg cgcggcgctc ggtcgagcgg
    13081 aaaacgagat cgttcagccg atggaagagt gacgtctcct ccgcgccctc ttcgaccagc
    13141 gtggtgatcg cagctttcac catcgccatc acgaggccgg ttgaaaggcc gtggcccgat
    13201 acgtcaccga tcgcaaccgc cagacgggtc ttgtcgatgt tgaagacgtc gtaatagtcg
    13261 ccgccgatcg atgcggtcgg ctcgaagtgt gcggagaacg acacgccgcg gaactgcgga
    13321 ccttctttag gcaggaggtt ccgctggatt gacgccgcga tctcgatctc actctgcaac
    13381 cgctcgttct cagcgacgtt cacgagcagc gcggcgatcg actcggtcat ccggtcgaac
    13441 gattgcgcca tctcgccgag ctggttgtgc ggcttcatgg cgatgcggta ggtgaagtcg
    13501 ccgcgctcga cggccttcgt gcccttctcg atgcggttga cggcgcgcgt gatggagaag
    13561 atcagcaccg cggcaaacag caccgccatc gcgtagaaaa cgagcagcag gacgacgagg
    13621 acggacatga actgcagaag acggctgacg tagttgtccg cttccgggcc aaagtaaaag
    13681 tgaaagaggt tctggaacgg attcacgatc atcgtgaaga tcgtgtgctt cccctcggcg
    13741 gtcggtcccc cgttccagtc ggtgaggtcc gtcaggtcgg gccagatgac ttttctgccg
    13801 acggaacgca tgaagaggtc ggtgaactga ctgtcgtcct ctccatcgaa gttgagtccc
    13861 ccatcaccct tctcgacccg aacaccgcgg cggtaacgag ccgttgtccg gtcgccgact
    13921 ttggtgagga tggtcattcc ggacttgtcc tgaaactgtt cgagccattc ggcgtcgagc
    13981 ggctcgacaa agacgatcgt ccgtacgggc gcaccttcct gttgcgatgc ctgacgcgac
    14041 gccacgagaa gtgcctgacc gtcccgccag accatgccgc tgaacgtcgt cgacttcagc
    14101 cacgacggga gcttgttgcc cgacgcgttg gccgtgtcgt agatctcgag tgccttcgtc
    14161 gcgccggaag gatgtttccc ggtgagtgag tactcgaacg cggaggattc caactggccg
    14221 aggctggcct gacgctcgcc gcgcaacgcg gcatgcgtca tcacgccgca gatcatgtag
    14281 ccgatcgtca gcaggagaat cgcaaagaga aagaacggca aaacgccgat gagggtgtag
    14341 ctcaggaaga gtcttcgccc cacgcgccag aagagttttc gcagcgtcca gcgaacaaag
    14401 acggcgaggc cgtaaagcgt cagcgcgatg acgagcaggc aagccagaaa cgtcgtgaac
    14461 ccgaagaacg cgccgccagg aaggttcagc ggccgcaaga caaaccgggt gatcagctca
    14521 ccggcgatga tgatgccgat cccgagcagc cacacgagga acagccgcgc tccgcggcgc
    14581 gatttgggcg gttcgggttt ttgttgtaca ggcagcatgt caggcgctgc tgagcgtata
    14641 cgaagaaacg atcccgcggt tccttagcgg ggtgtgggga gtcgggggtg gggggtcgga
    14701 gataaggcct cgccagcagg ggggcgaccg tcgtggttga gagcgggtga cgaagtcgga
    14761 ggctggtttt gctacgaccc ccgactcccg acccgctacc ccgcgccgtc ttatctatcg
    14821 cagcgctgtt gagatcgtcc tccgggcggc tgcatcagag atcagagaaa agttcgcttc
    14881 tcccaccccc aacaccccac tccccacccc cgactcaaaa cgtccggaac tgcagttccc
    14941 tcggctccag ttcggccacg ttcatgcggc tcgtcgtacc gtcgcagtac gcccagtcgt
    15001 ggctgatgcc ggtgaccatg agagcgtcgt cgggacagag ctcgccgagg gcttcgccta
    15061 aggtttgaac gtccgtggtg tcgggggatt gaacgacgaa cgtcgattcg aacggcagct
    15121 cgacgacgac gccgaggcgt gcaatggcgt cgtgcagctc gctgtagtcc gcgccgggag
    15181 tgaggctgtg gcggatgagc aggaagtcgc gcgaggtggt gaattgggcg cagccgacga
    15241 gaacgcgggc ctcgatgccg acctccatcc tcaagtagcg gacgatcagc tcggcgtggc
    15301 caccgtcagg aacccagaca tgccagctct gatcgaagtt cggctcagct cccaggcggc
    15361 gcatggcgtt cgaaacgtca atggtcagct cgccctcgta gcaaacctgg aacagcttgg
    15421 tttctcccat cacaccctcc caccataact gtcagtagac ggacaaatga tagcacggtg
    15481 ccttcgagat ccgctggccc gcttcttgag ttatttgaat ggcgaaggag acccaaccat
    15541 gcgattcaac gacgacgaag taaaaggcaa gatcgaccag gcgagcggag ccgtgaagga
    15601 gaacatcggc cgcgccagag gcgatcaggc tctggagaac gaaggcgccg accagcgcgc
    15661 cagcggcaaa ttcgaagccg gcttcggcaa gacccgccgc aagatgggcg aggctgtaaa
    15721 agacctcggc gacaagctgg ggaaatagcc taccgggaac tcaagaatcc ccgcgcatgc
    15781 cacgcgggga ttcgttgtgc cgtcattgaa gctacgtgtc gatcaggatc tccgggtcga
    15841 gtgttttgta tccggttgac tcaacagcgt attccagggt tctgcctggg ttcgtgaatg
    15901 gcccgcactc cgccatccag actccaccag tgttggtcgt gacagggttc ggccaatccg
    15961 atccaggctt gaacttgatc tggaaattgc cttcacgaca tgaccactgc accttcgttt
    16021 tacccgatac cgagaccctg acgtgtgagt cgtcgacgct gatcgtcttg gtggcgctgt
    16081 ccagcgtcac tcttacatta gccatactgc ctccctcttt ctcaatgcta aaggacgcct
    16141 ctattgtact cgtgtcgcaa atattctttt gtactccgca gactcgcgca aaattgagaa
    16201 ctcggggtcg cgctcgatct cgatacggct ataaccttgg tccactgcac gtttcaggta
    16261 ttgggcagca gcttgttttg cacccgccac attggcgact acaccagcgt tgtacatgac
    16321 attgatgtct gtgggatcgt cggaaatagc gcgggagatc tggccggtgg cactgcgcag
    16381 atcaccgcgc ttggcaagac aagctgccag tcgagcacga gtaacgttat cggcggcatt
    16441 caactgcaga tctg
//
LOCUS       DATLVW010000704         4867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_278854, whole genome shotgun sequence.
ACCESSION   DATLVW010000704 DATLVW010000000
VERSION     DATLVW010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278854"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1177
                     /locus_tag="VNN25_20440"
     CDS             92..1177
                     /locus_tag="VNN25_20440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH93960.1"
                     /translation="MLKVECRALNVERRPRSASRPRFALHSSFFTLLLLTSCITITPK
                     TQHASPSASVIPNVPLQHWGIESCGAGSLSTVLQHYGDATTMRAWDDTLPKTRGGVLT
                     IDMLIAARKSGFDAQLITGTPAIISDELREGRPVILMLQVVDSPGHHYDFFHYIVADG
                     IDPDRGLIRTQFGDGNGRWTTFDRLEKAWAGGGHTAILIHPRAAAEALRAAVALEEAG
                     KYAEAARAYQSILDQHPDSLLAWTNLGNAEVQLGNRNDAENAFRKALAIDPSSRDALN
                     NLAWLLYEAKRYDEAESLARKAVALQGPDSYLMLDTLARVLAAKGSCNEALTTFRAAI
                     DAVPPTRTAARGDLEKAMADARTGCRG"
     gene            1249..2370
                     /locus_tag="VNN25_20445"
     CDS             1249..2370
                     /locus_tag="VNN25_20445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXH93961.1"
                     /translation="MFSSRARWNAATNRLTFARQAYTGDLLDLTVSNPTKAGIPYPLD
                     ELSETMARAARAPYDPQPLGLASAREAVARELGCDAADIVITASTSEAYSYLFKLLCD
                     PGDAVLTATPSYPLIEHLAALELIEMHSFPLELHQRWEIDVDRLRSALTERTRCILVV
                     NPNNPTGSYVSAREQNAIASLGTPVISDEVFHPFAFGESGPSFVRDDVLTFTLGGLSK
                     SAGLPHYKLGWIRVSGPGKAEAIHAIEWIADNFLSVATPVQVALPEILAIAPQIRDAI
                     RERTAANLGSLRALLATAPATRLLPVEGGWSAVLRVPRFESDEALALRLIERHGVIVH
                     PGYFFDFATEGHVVVSLLTPPDAFENGVASLLEAVNNQG"
     gene            2423..3595
                     /locus_tag="VNN25_20450"
     CDS             2423..3595
                     /locus_tag="VNN25_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH93962.1"
                     /translation="MREHWLLEPHVTYLNHGSFGATPKVVLAKQNDLRTHLEREPVRF
                     MVRELEPLLDEARATLGAFIGADPASLAFVPNATTGVNAVLRSIDLDKHDELLVTSHE
                     YNASRNALEYVAGLAGAKVVPVDIPFPIASSGEIVERVLAGVNDRTRLLLIDHVTSQT
                     ALIFPIERIIAELKQRGIDTLVDGAHAPGMFPLNLDTLGAAYYTANLHKWVCAPKGAA
                     FLYVAPNRRLGIHPVVISHGYNATRTDRSRFHLEFDWPGTFDPTAWLCVPEALRFMGS
                     IVEGGWPEVMRRNHEMALRARDLLCPRLGIQNPAPDDMLGSMAAMPLPDGEAYLTTSL
                     YGDPLQDALLFEHGIEVPIVPWPRAGNRVLRVSAQVYNAIGDYEKLADALGGLLRG"
     gene            complement(3670..>4867)
                     /locus_tag="VNN25_20455"
     CDS             complement(3670..>4867)
                     /locus_tag="VNN25_20455"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXH93963.1"
                     /translation="AFLSVSVALLLSLLGGALFGQATQKNNVYRYISIFTEVYELVQH
                     NYVEQVPADQLMDGAFSGVTDAIDEFSYYVPPPQMAAYKNFSDVEDNGVGLIVTKRFG
                     YAFVIAPVSGSPAAKAGIERGDFIEKIDGQPTQKMAVWQVRNLLRSGKPMHLQVLRGG
                     QTKRDEFVLQQAAFHPIALETKTLGGVAYIKIPYFEKGTAAQLATALENVRKSGTRKL
                     IVDVRGNAGGDVDEAINAADELLTSGLITSLAGKVEAKKWQADRNTAYDGDVEVLTDG
                     STASGAEIFAAAIHGNQRGKVVGVTTYGKSIVQKFMSLPSGGGVYMTVAHYTTPDMKP
                     IKSGGIRPDVMVDLTALVLRDPETKSVLAPHGAPKAAVKPPEDLILEKALQLFNEPAA
                     AAKKAA"
BASE COUNT          871 a         1598 c         1467 g          931 t
ORIGIN      
        1 aactgtttcg ccgtagcttc cgggcgagcg ggctggttgg gccgctcgct cggtgttttt
       61 gcggggtttg tagagaatgc ggagagccga aatgttgaaa gttgaatgca gggcgctgaa
      121 tgtcgaacgc agaccgcgat ccgcgtcccg tccacgtttc gctcttcact cttcattctt
      181 cactcttctt ctcctcacct cctgcatcac catcaccccc aaaacgcagc acgcctcccc
      241 gtccgcatcg gtaatcccca acgttccgct gcagcattgg ggcatcgagt cgtgcggcgc
      301 gggatcactg tcgacggtgc tgcagcatta cggcgacgcc acgacgatgc gcgcgtggga
      361 cgacacgctg ccgaagacgc gcggcggtgt cctgacgatc gacatgctga tcgcggcgcg
      421 aaaatcgggc ttcgacgcgc agctcatcac aggaacaccg gcgatcatca gcgacgagct
      481 tcgcgaggga cggccggtga tcctgatgct gcaagtcgtc gactcgccgg gtcaccacta
      541 cgatttcttt cactacatcg tggccgacgg aatcgacccg gatcgcggcc taattcgcac
      601 gcaattcggc gacggcaacg gccgctggac gacgttcgac cgtctcgaaa aagcgtgggc
      661 cggaggaggc cacacagcca tcctgatcca tccgcgcgcc gcagccgagg cgctccgcgc
      721 agccgtggca cttgaggaag caggcaagta cgctgaggcc gctcgtgcgt atcaatcgat
      781 cctcgaccag caccccgact ccctcctcgc ctggaccaac ctcggcaacg cagaagtgca
      841 gctcgggaac cgtaacgacg ccgagaacgc gttccgcaaa gcgctggcga tcgatccgtc
      901 gtcgcgcgat gcgctcaaca atctcgcctg gctgctctac gaggcgaagc gctacgacga
      961 ggcggagtcg ctggcccgca aggcggtagc gttgcaaggt cccgattcct acctcatgct
     1021 cgacacgctt gcgcgcgtgc tggccgcgaa gggatcgtgc aacgaagcgc tgacgacgtt
     1081 ccgcgcggcg atcgatgcgg tgccgccgac acggacggcg gcgcgcggcg atctcgagaa
     1141 ggcgatggcg gacgcgcgga ccgggtgccg ggggtagtca cgcgaagacg cgaaggcgcg
     1201 aagcagtttt cttcgcgtct tcgcgtcttc gcgtgaaaaa atccaagcgt gttctccagc
     1261 cgcgcccggt ggaatgccgc cacgaaccgc ctgacgtttg cccggcaggc ctacacgggc
     1321 gacctgctcg acctgacggt ttccaatccg accaaagccg gcatccctta tccgcttgac
     1381 gagctgtccg agaccatggc ccgcgcggca cgcgcgccgt atgacccgca gccgctcggg
     1441 ctcgcgtccg cgcgcgaggc ggtcgcgcgc gagcttggtt gtgatgccgc agacatcgtc
     1501 atcaccgcgt cgaccagcga ggcgtattcg tacctcttca aacttctgtg cgacccaggc
     1561 gacgcggttc tgacagcgac gccgtcctat cccctgatcg agcaccttgc cgccctcgaa
     1621 ttgatcgaga tgcattcctt cccgctcgag cttcatcagc gatgggagat cgatgtcgat
     1681 cggctgcgca gcgcgctgac cgagcgcacg cgctgcatcc tggtcgtcaa tcccaacaac
     1741 ccgaccggct cgtacgtctc tgcacgcgag cagaacgcga tcgcctcgct tggcacaccg
     1801 gtgatctcag acgaggtctt tcaccccttc gcgttcggcg agagtggccc gtcattcgtt
     1861 cgcgacgacg tcctcacatt cacgctcggc gggctctcca agtctgccgg actcccccac
     1921 tacaagctcg gctggatccg cgtgagcggt ccggggaaag cggaagccat ccacgcgatc
     1981 gagtggatcg cggacaactt tctctccgtc gcgacgccgg tgcaggtggc gcttcccgag
     2041 atcctggcaa tcgcgccgca gatccgtgat gccatacggg agcggaccgc ggccaaccta
     2101 ggttcgctgc gtgcgctgct cgcaaccgcg cctgcaacgc gcctccttcc tgtcgaagga
     2161 ggctggtcgg cggtattgcg ggttccgcgt ttcgaatctg acgaagcgct cgcactgcgc
     2221 ctgatcgagc gtcacggcgt aatcgttcat cccggctact tcttcgattt cgcgaccgag
     2281 gggcatgtgg tggtgtcgct cctgacgcca ccggacgctt tcgaaaatgg cgtcgccagc
     2341 cttctcgaag cggtaaacaa tcagggttga tcgaaactgg gcacttgccc attcatccgt
     2401 catccttcat cgttcatccc tcatgcgaga acactggctc ctcgagcccc acgtcacgta
     2461 tctcaatcac gggtcctttg gtgccacgcc gaaggttgtg ctggcgaaac agaacgacct
     2521 tcgcacgcat ctcgagcgcg agccggtgcg cttcatggtg cgcgagctcg agccgctact
     2581 cgacgaagct cgcgctacgc tcggggcatt catcggcgcc gatcctgcct cgctcgcctt
     2641 cgtgcccaat gccaccacgg gcgtgaacgc cgttctgcgc tcgatcgatc tcgacaagca
     2701 cgatgaactg ctcgtgacgt cgcacgagta caacgcctca cgcaatgcgc tcgaatacgt
     2761 ggccggtctc gcaggagcga aggtcgttcc ggtcgacatc cccttcccga tcgcatcatc
     2821 cggcgagatc gtcgagcgcg tgctggccgg cgtcaacgac cgcacacgtc ttctgctcat
     2881 cgatcacgtg acgagtcaga ccgcgctgat cttcccgatc gagcggatca ttgcggagtt
     2941 gaaacagcgc ggcatcgaca cgctcgtcga cggcgcgcat gccccgggca tgtttccgct
     3001 caacctcgac accctcggcg ccgcctacta cacggccaat ctccacaaat gggtttgcgc
     3061 gccgaaggga gcggcatttc tgtacgtcgc acccaaccgc cggctcggaa tccatcctgt
     3121 cgtgatcagc cacggataca acgccacgcg caccgatcgc agccgcttcc atctcgagtt
     3181 cgactggccg ggcacattcg atccgaccgc gtggctctgc gtccccgagg cgctgcgatt
     3241 catgggatcg atcgtggaag gaggatggcc cgaggtgatg aggcggaatc acgagatggc
     3301 gctgcgagcg cgcgatttgc tctgcccgcg cctcggcatc cagaacccgg cgccggacga
     3361 catgctcggc tcgatggcgg cgatgccgtt gccggacggt gaggcatacc taacaacctc
     3421 gctctacggc gatccgctgc aggacgcgct gcttttcgag cacgggatcg aagtgccgat
     3481 cgtgccgtgg ccgcgcgcag ggaaccgcgt gctgcgagtc tctgcgcagg tttacaacgc
     3541 gatcggggac tacgagaaac tcgcggatgc gctcggtggc ttgcttagag gctgatcacc
     3601 acagagacac agaggtcaca gagaaaggct tctatctctg tgacctccgt gtctctgtgg
     3661 ttaatgcttc taagccgctt tcttcgccgc cgccgcaggt tcgttgaaca actgcagcgc
     3721 cttctccagg atgagatcct cgggtggttt tactgcagcc ttcggagctc cgtgcggcgc
     3781 gagcacgctc ttcgtttccg gatcgcggag caccagagcg gtgaggtcaa ccatgacgtc
     3841 aggccggatg cctcccgatt tgatcggctt catatcgggc gtcgtgtagt gggccaccgt
     3901 catgtagaca ccaccaccgc tcggcagtga catgaatttt tgcacgatcg attttccgta
     3961 cgtcgtcacg ccgacgactt tgccgcgctg gttgccgtga atcgcggcgg cgaaaatctc
     4021 ggcacctgaa gcagtcgagc catcggtgag aacttcgacg tcaccgtcgt aggcggtgtt
     4081 ccggtcggcc tgccacttct tcgcttcgac ctttcctgcc aatgaggtaa tgaggccgct
     4141 cgtcagcagc tcatcggccg cgttgatcgc ctcgtctacg tcgccgccgg cgttcccgcg
     4201 aacgtcgacg atcaacttgc gcgtgccgct cttgcgcacg ttctcgagcg ccgttgccag
     4261 ttgcgcggca gtccctttct cgaagtacgg aatcttgatg taggcgacac caccgagagt
     4321 cttcgtctcg agggcaatcg gatgaaacgc cgcctgctgg agaacgaact catcgcgttt
     4381 ggtctggcca ccgcggagaa cctggaggtg catcggcttg ccggagcgaa gcagattgcg
     4441 cacctgccag acagccatct tctgcgtcgg ctgtccgtcg atcttctcga tgaaatcgcc
     4501 acgctcgatc cctgctttcg cggcaggcga tcctgaaacc ggtgcgatga cgaacgcgta
     4561 gccaaaacgc ttcgtgacga tgagaccgac gccgttgtct tcgacgtcgc tgaagttctt
     4621 gtaggccgcc atctgcggcg gcggcacgta gtagctgaac tcgtcgatgg catcggtcac
     4681 gcccgagaac gcgccgtcca tcaactgatc ggccggcacc tgctccacgt agttgtgctg
     4741 cacgagctcg tacacttccg tgaagatgct gatgtagcgg tagacgttgt tcttctgcgt
     4801 ggcctggccg aagagcgcgc cgccgagaag cgagagaagc agggctaccg aaacggagag
     4861 gaatgcg
//
LOCUS       DATLVW010000705         4527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_399720, whole genome shotgun sequence.
ACCESSION   DATLVW010000705 DATLVW010000000
VERSION     DATLVW010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399720"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VNN25_20460"
     CDS             complement(<1..697)
                     /locus_tag="VNN25_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93964.1"
                     /translation="MSDDLGIDVQGVSKDFGTFRALDDITLQVPRGEIFGLLGPNGSG
                     KSTLIRILCGLLVPSGGRATVDGFDVESQGEEVRQHIGYVSQAFSLYRDLTVQENLDF
                     FSSIYRLKGAERAERIDWAINLTHIGPYRDRLAGALSGGWKQRLALAAALMHQPRVIF
                     LDEPTAGIDPVARRELWDLLFELAGGGVTMFVTTHYMDEAERCASVAYLYMAKLIVAG
                     RPDTLKELPEVTPE"
     gene            complement(694..1545)
                     /locus_tag="VNN25_20465"
     CDS             complement(694..1545)
                     /locus_tag="VNN25_20465"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH93965.1"
                     /translation="MLSGALEARTVNVGSLVGGRVTKTLIDEGMTVVPGQLLVTLETE
                     TIDRQLSEQRAAIKEANAALAKALAGPRPEEIAKAAAIASNDEVERRRWERLYRYGIV
                     AKEMAEDAATKAKTSADDLRILQKGTRKEDIEAARAAVESQQRRLDTLMKQRAETNVV
                     STVSGVVQSFGLRVGDIVAPNQAVAEILESSQLWVRVYVPETELGLVPVGKTVRISID
                     TFPNETFTGHVASISDQGEYTPRNVQTRAQRAEMVFGVKVLVDPNPKLKAGMAASADL
                     GVKGRPE"
     gene            complement(1463..2281)
                     /locus_tag="VNN25_20470"
     CDS             complement(1463..2281)
                     /locus_tag="VNN25_20470"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXH93966.1"
                     /translation="MGRPPRIRREQILESARRVFAAKGFDAATLADIAGEIGVTPAAI
                     LRHVDSKAALFSEAMQSGGLIDPPACVLELANIDPATDPRIVLRRLAQEVVPFISSII
                     TTRIVVATYESARRTSLVLPFNAEEENPPKKAFRLLTNYMRRAAKAGVLHVRDPRAAA
                     LLFMGSLQGYVLFHYVLKVEPIFPLAGYVDALIDLWSEGAIVGGIDARRKETKTDASN
                     RDRADRDRNRSSRPAAVSARKPKARRDRAQRSPRSADGQRRVAGRRARNKDADR"
     gene            complement(2389..2697)
                     /locus_tag="VNN25_20475"
     CDS             complement(2389..2697)
                     /locus_tag="VNN25_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93967.1"
                     /translation="MAISKKPLLFSCVIQFRVIQIQTLIQGVFIRQTKHIRFLFAIAL
                     LVAIVPFSAVRNTYYAVATTGGVPTKFTLQDIPTSNPFGFPTSSFGPRIMQFAAKVVF
                     "
     gene            2690..4054
                     /locus_tag="VNN25_20480"
     CDS             2690..4054
                     /locus_tag="VNN25_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXH93968.1"
                     /translation="MAIASINPATGETVRTFEALDDAAIEASLARASSAFRVNRGRSF
                     AERATRMRRTAELLEGRKNDLGRLMTTEMGKPVKAAVAEVVKCATTCRYYAEHAEGYL
                     ADETVKTDAKESFIRYEPLGPVLAVMPWNFPFWQVFRFAAPALMAGNVALLKHASNVP
                     QCALAIEEVFRDAGFIDYEFQTLLIGSPQVESIIGDERVRAVTLTGSEPAGASVASIA
                     AKHIKKAVLELGGSDPFIVMPSANLEDAVPTAVKARIVNNGQSCIAAKRFIIHESIAD
                     EFEKRFVSAMEALKVGDPLDESTDIGPLATPQIVDDIEAQVNQSVAAGAHLLTGGKRL
                     GARGNFYAPTVLAQIPDEAPAAHEETFGPVASLFRAKDVDDAIRIANDTPFGLGASVW
                     TTDRTEMDHFIAGIDTGQVFINAMVASDPRVPFGGTKHSGFGRELGAYGIREFVNVKT
                     VWVG"
     gene            complement(4083..>4527)
                     /locus_tag="VNN25_20485"
     CDS             complement(4083..>4527)
                     /locus_tag="VNN25_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93969.1"
                     /translation="AHRDYFDETIRSDFEDSLDLDEAMRLGREGQNRWDYLLSHAPSD
                     EVVAVEPHSAKQDEISTVIKKRSAAREHLRPHLRDGVNISRWLWVASGRVHFADTEKA
                     RRRLDQHGIEFVGQRVMARHLGAVAIKEPTKRRRVNPPDGSKSRR"
BASE COUNT          855 a         1435 c         1374 g          863 t
ORIGIN      
        1 cttccggtgt gacctccggc aactctttga gcgtgtccgg ccgcccggcg acgatcagct
       61 tggccatgta gagatacgcg accgacgcgc agcgctccgc ttcgtccatg tagtgcgtcg
      121 tcacgaacat cgtcacgccg ccgccggcaa gctcgaagag gagatcccac aactcgcgcc
      181 gcgccaccgg atcgatgccc gcggtcggct cgtcgaggaa aatgacgcgc ggctggtgca
      241 tgagcgccgc cgcgagcgcg aggcgctgct tccatccgcc ggacaacgcg ccggcgagtc
      301 tgtctcggta ggggccgatg tgcgtgaggt tgatcgccca atcgatccgc tcggcgcgct
      361 ccgctccttt gagacgatag atggaactga agaagtctag gttctcctgc acggtcagat
      421 cgcgatagag cgagaaggcc tgcgagacgt agccgatatg ctggcgaacc tcctcgccct
      481 gactctcgac atcgaagcca tcgaccgtgg cccggcctcc ggacggtacg agcagtccgc
      541 agaggatgcg gatcagcgtc gacttgccgg agccgttcgg gccgaggagt ccgaagatct
      601 cgccacgcgg cacctgcagc gtgatgtcgt cgagcgcacg aaaggttccg aagtccttcg
      661 acacgccctg cacgtcgatg ccaaggtcgt cgctcactcc ggccgtcctt tgacgccgag
      721 gtcggccgaa gccgccatgc ccgccttcag cttcggattg ggatcgacga gaactttcac
      781 gccgaaaacc atctcggcgc gttgggcgcg cgtctgcacg ttgcgcggcg tgtactcgcc
      841 ctgatcgctg atcgaggcta catggcccgt gaacgtttcg ttcggaaatg tgtcgatcga
      901 aatgcgcacg gtctttccga ccggaaccag gccaagctcc gtttccggca cgtagacgcg
      961 cacccagagc tggctggatt cgaggatctc tgccacggcc tggttcggcg cgacgatgtc
     1021 gccgacgcgc aatccgaacg attgcacgac gccgctcacg gtcgagacga cgtttgtctc
     1081 cgcgcgctgt ttcatgagtg tgtcgagccg ccgttgttgc gattcgacgg cggcgcgcgc
     1141 cgcttcgata tcttccttgc gtgttccttt ctgcaggatg cgaagatcat ccgccgacgt
     1201 ctttgctttt gtcgcggcat cctcggccat ctctttcgcg acgatgccgt agcgatagag
     1261 acgctcccaa cgacggcgtt ccacctcatc attgctggcg atcgcggcgg ctttggcgat
     1321 ctcttcaggg cgcggaccgg caagcgcctt cgccagcgca gcgttggctt ccttgatcgc
     1381 ggcgcgttgc tcgctgagct gtcggtcgat cgtctcggtc tccagggtaa ccagcaactg
     1441 gcccggaacg accgtcatcc cctcatcgat cagcgtcttt gttacgcgcc cgccgaccag
     1501 cgacccgacg ttgaccgtcc gcgcttcgag ggctccgctg agcacgatct cttctggctt
     1561 tcggctttcg cgcagataca gcagcaggcc ggctgctacg attccgatca cgatcagcgc
     1621 gatcacgatt cgaagcatcg gtcttggttt cttttctgcg ggcatcgata cctccaacga
     1681 tggctccttc cgaccagaga tcgatgagtg catcgacgta gccggcgagc ggaaaaatgg
     1741 gctcgacttt caggacgtag tggaagagca cgtatccctg cagcgatccc atgaacagca
     1801 gcgcggcagc acgcggatcg cgaacgtgca ggacgcctgc cttcgccgcg cggcgcatgt
     1861 agttggtgag gagccggaac gccttcttcg gaggattctc ctcctcggca ttgaagggca
     1921 gcacgagcga agtgcgacgc gcgctttcgt acgtagccac gacgatccgc gtggtaatga
     1981 tgctgctgat gaaaggcacc acttcctggg ccagccggcg aagcacgatg cgcgggtcag
     2041 tcgccggatc gatgttcgca agctccagca cgcaggcggg aggatcgatc agtccgccgc
     2101 tctgcatcgc ttccgagaag agcgccgctt tggaatcgac gtgccggaga atcgccgccg
     2161 gcgtcacgcc gatctcgccc gcgatatcag ccagcgtcgc cgcgtcgaac cccttcgcag
     2221 caaatacacg acgcgcgctt tccaggatct gctcgcggcg gatacgtggc ggtcgtccca
     2281 tttgtaaatg aacgtatatt tacaaacggc cgttgtcaag ggggctggac gcaaaaaggc
     2341 cgccggaaac aacccggcgg cccccgtgcg aaactgcgac gacggatctt agaacacgac
     2401 cttcgcagcg aactgcatga tgcgtggacc gaacgagcta gtcgggaacc cgaacgggtt
     2461 cgacgtcggg atgtcctgca gcgtgaactt cgtcgggaca ccgccggtgg tcgcaaccgc
     2521 gtagtacgta ttgcggacgg ccgaaaacgg cacgatcgcg acgagcaggg ctatcgcaaa
     2581 caggaagcga atatgcttcg tctgacggat gaagactcct tgtatgaggg tttgaatttg
     2641 aatgactcga aactggatta cgcaggagaa taacagaggt tttttgctga tggccatcgc
     2701 atcgatcaac ccggcaactg gggaaactgt ccgcaccttc gaagccctcg atgatgccgc
     2761 gatcgaggcg tctctggcgc gtgcatcgag cgcatttcgt gtcaatcgcg ggcgaagttt
     2821 tgccgagcgc gcaacgcgca tgcgccgcac cgcggagttg ctcgaagggc gcaagaacga
     2881 cctcgggcgc ctcatgacga ccgagatggg caagcctgtc aaggccgcgg tagccgaggt
     2941 ggtgaagtgc gcgacgacgt gccggtacta cgcggaacat gccgaagggt atctcgccga
     3001 tgaaacggtg aagacggatg cgaaggagtc gttcattcgc tacgaaccgc tcggccccgt
     3061 cctggcggtg atgccgtgga actttccctt ctggcaggtc ttccgcttcg ccgctccggc
     3121 tctgatggcc ggcaacgtcg cgctgctcaa gcacgcttca aatgtcccgc agtgcgcgtt
     3181 ggcgatcgag gaggtcttcc gcgatgccgg cttcatagat tacgagtttc agacgttgct
     3241 gatcggatcg cctcaggtgg agtcgatcat cggcgatgag cgcgtccgcg ccgtaacgct
     3301 caccggcagc gagcccgcag gtgcgagcgt ggcctcgatc gccgcgaagc acatcaagaa
     3361 agcagtcctc gagctaggcg gcagcgatcc gttcatcgtc atgccgtccg cgaacctcga
     3421 agacgcggtg cccacggcgg tcaaagcaag aatcgtcaac aacggtcagt cctgtatcgc
     3481 cgcgaaacgc ttcatcattc acgagtcgat tgcagacgaa ttcgaaaagc gcttcgtgtc
     3541 ggcgatggaa gccttgaaag tcggcgatcc gctcgacgaa tcaaccgaca tcggcccgct
     3601 agccacaccg cagatcgtcg acgacatcga ggcgcaggtg aaccaatccg tcgccgccgg
     3661 tgcccacctc ctcaccggcg gcaaacgcct cggcgcccgc ggcaacttct acgcgccaac
     3721 agtcctcgcg caaatccccg atgaggcccc cgccgcccac gaagaaacgt tcgggcccgt
     3781 agcctcgctc ttccgcgcaa aggatgtcga cgacgccatc cgcatcgcca acgacacccc
     3841 tttcggccta ggcgcctcag tctggacaac cgaccgcacc gaaatggacc acttcatcgc
     3901 aggcatcgac accggccagg tctttatcaa cgcgatggtc gcctcagacc cacgagtccc
     3961 cttcggcggg accaagcact ccggatttgg gcgggagtta ggggcgtacg gcattcgcga
     4021 gtttgtgaat gtgaagacgg tttgggtggg gtagagaact ccgtggggct acttcgcaac
     4081 gttcagcggc gtgacttgga accgtcgggg gggtttaccc gccgcctctt tgtcggctcc
     4141 tttattgcca ctgcgccgag gtgcctggcc atcacccttt gcccaacgaa ctcaatgccg
     4201 tgctgatcga gtcgcctcct ggccttttcg gtgtcggcga agtgaactct cccggaagcc
     4261 acccataacc acctcgagat gttcacgccg tcgcgaaggt gcgggcgcag gtgttcccgc
     4321 gccgccgatc gcttcttgat cacagtcgag atctcatctt gcttcgcaga gtgcggttca
     4381 actgcgacaa cctcatcgct tggcgcgtgg ctgagtagat agtcccatcg attctgtcct
     4441 tcacgtccga gcctcatggc ttcatcaaga tcgagactat cctcgaagtc tgaccgaatg
     4501 gtctcgtcga agtagtcacg gtgcgcc
//
LOCUS       DATLVW010000706        12592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_278883, whole genome shotgun sequence.
ACCESSION   DATLVW010000706 DATLVW010000000
VERSION     DATLVW010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12592
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278883"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="VNN25_20490"
     CDS             complement(<1..1744)
                     /locus_tag="VNN25_20490"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH93970.1"
                     /translation="MRKLARFALAALFVTSSSTIAHAAITGTIIDMDGKPVAGATIRA
                     FAPESSAALRARLVAGKADRDPGATAKSSDSGTFSIDLKSSPAVDLEIEAPDHPRTTI
                     ATVDGDDIGVIQLAAPPVAKIRVTSGGKPVANAIVLSGSEVRKTDDAGETPVFGIGSV
                     YVVHPDFAPGSRRDMNGAISSLHEIKLSRGVSVRGRVVNGAGPVPHAIVSVNGWPLAE
                     SADDGTFVVAHAPDGWQLLTAARGNEIGAATRPAKTGAVEIRLAKGETFTGTVRDSVT
                     GAAVAGARMSLNEGADSLPATAVSDAKGRFTFGPLLSRTYQIEGMHPSYGIASAAVLV
                     PHVTTRAFAALPVAHARGRVVDEEKKPVAGAIVTSPVTTGPRRPSTLTNAAGEFAIRV
                     VTSATPFALPIYASKRGYAGGMLEARKWQPGETRNDIVITLVRGFAIAVRVRDRQHQP
                     VEHAYVNLTRTGDGAQQGFFAPAACDDPTRQDCRRTSADGSVVFRTSEGPHSVFVTGD
                     EVAPKRVPSVTLTARSEPLVVEVDRGVTISGRVVHADGTPVAGAIVELPRSPIAPRNA
                     TADSDGAFTLVGIAA"
     gene            complement(1762..2406)
                     /locus_tag="VNN25_20495"
     CDS             complement(1762..2406)
                     /locus_tag="VNN25_20495"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXH93971.1"
                     /translation="MQDKALFDAIRENDAAAVARLLDAEPALIGARQNGITPILFAAY
                     NGHAELAQLFLDRGATLTFGEACALGRTELAAQMLDADSALLDSYSDDGFPAIGLAVF
                     FRHPELARTLIERGADVNAAARNAFRVAPVHAAVAVRDPAIMQLLIKHGADVNARQQM
                     GYTALHTAAQHGDDVMLDLLLAAGADPRAAGDDGKTPADLAAAQGHTAIEKRLS"
     gene            2771..2989
                     /locus_tag="VNN25_20500"
     CDS             2771..2989
                     /locus_tag="VNN25_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93972.1"
                     /translation="MSEKTAKVSKTVTVSERNLQSAALRLLPKNNKLVSPEVDYLRRV
                     LGERATQQQIDEKVQQVRKLPWSEIVHE"
     gene            complement(3253..4317)
                     /locus_tag="VNN25_20505"
     CDS             complement(3253..4317)
                     /locus_tag="VNN25_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXH93973.1"
                     /translation="MTNYQFDPRLAFARTLPSRYYFDRDVLDAENRNVFGRTWQLVGH
                     ADRVRDPGQFFTTTIANEPVLVVLGSDGVLRAMSNVCRHRAGPVAKGEGKRPVLQCGY
                     HGWTYALDGRLLQTSEMSGVECFERDDFSLPRFRVEVWNELVFVNLDTDAEPLTEFLG
                     DLVSRLAPRNYEGYRLAARKEWSLECNWKVYVDNYLEGYHIPIVHPGLFREIDYPNYR
                     TETRDNYSIQVAPLKRPDRIRVNDAGDDVDYFWVWPNLMLNVYPDNFSTNLIIPLGPT
                     RTLTLFEWYFRDPDAPAERQRIDETVAFSDEIQLEDIDICEAVQRGLASSTYDNGRYS
                     PSRENGVHHFHGLYANSMGI"
     gene            complement(4314..5612)
                     /locus_tag="VNN25_20510"
     CDS             complement(4314..5612)
                     /locus_tag="VNN25_20510"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXH93974.1"
                     /translation="MPPRAKLLTVLGVTFGLAVTVGNMIGAGILRTPGDIATQLPSFW
                     PFLSVWVIGGLYALLGANALAELGTLVPESGGQYVFVRRALGDYAGLVVGWSDWISTC
                     GTTAAVSIVVGEYALVLFPALRGIEPTALTVIAILTLIQWFGVRWGGWTQDATSLLKA
                     LAFLFLIGACFYLGTRHPTASLIEADHEGSLAIAFILALQAVIYTYDGWTAVIYFSEE
                     VKDSGRNIPRAMFGGVFAVMAIYLLVNIAFLRVVPLGSLAGQKLAAGVVAQHLFGAYG
                     DTIVRIIMIVSLISAVNSNVLIAPRVLFAMSRDKLFWRGAAEVNKGGTPDIALLFSSI
                     LAAVFVISGKFEQVIAILAFFFVANYALSFASLFAMRRSSPDAPRPFRAIGHPFTTGL
                     AFLASIAFLIGAVISDTRNSLYALGVLILTIPLYLLVRRK"
     gene            complement(5758..6678)
                     /locus_tag="VNN25_20515"
     CDS             complement(5758..6678)
                     /locus_tag="VNN25_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93975.1"
                     /translation="MRRLAFLPALIALTLPLAAVSHSSHSTYSNHGLSVTTDDWEDIR
                     DCSALHVTYNDRSVPVVEENVPVGSLRSLKVRSDRNGSIRVLASTNSSFAVKACKASA
                     LGNVNDLRVNVSGNEVSADRAETDGRSIIYLLVFAPRGAAIDLDASNGPISIDGVDGE
                     ITAHALNGPIAVKASSGTLDINTQNGPISFAGASGNVKLRATNGPISVKLTGGSWGAG
                     SLDASTENGPMSLKLPSGYRSGVLVETDGNSPVSCRAAGCAAAKKAYASSEDDNDFGP
                     RWPRRIELGSGTRAVSLATHNGPVSVKEAD"
     gene            6792..7733
                     /locus_tag="VNN25_20520"
     CDS             6792..7733
                     /locus_tag="VNN25_20520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH93976.1"
                     /translation="MPGFLKRAARRLPLLRGLHRYILALEAERDQLRADLAHWKTWMS
                     PGHFYSPIPSLDEIRSRESEIFAPAPPALPGIDLREKDQVALVRELAAFYPALPFPRQ
                     QSPSTRYWFENWAFSYADAIFLYSMLRHLRPHRVIEAGSGFSSAAMLDTADRWLPDTS
                     FTFIDPDTATLDALLRPGDRERVTILPLRLQDVPLTTFDALSANDILFVDSTHVSKTG
                     SDVNRILFDILPRLASGVYIHFHDVFYPFEYPKEWVYEGRAWNEDYILRAFLEFNDSF
                     EIVLFGTWLARFHRELLDQQMPLTRENPGGSLWLRRK"
     gene            complement(7853..9715)
                     /locus_tag="VNN25_20525"
     CDS             complement(7853..9715)
                     /locus_tag="VNN25_20525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXH93977.1"
                     /translation="MYALRTAVLAVLVTTALVAQPATDLNMAQQALAAADSAGAPAFA
                     KTLYDDAVYRARFAEQNWNSSKPAIRGQAQMSAIEATVEAQAAAAKARWLSTNSAIRG
                     LQSDISRFGGTSPVILLPEESPMVEFHRGGTTKEQIAAAQFAVDQAKVAGALDTVPDN
                     DIRIAEGYIDSAKRVSRNTSESEPGDDLAFRAEMLGRRAFYLARLAQSSKFLPSVQLD
                     RTRLAQQMAERQAAADRAQREEAERRTAAMQQQLAAEQANRQAQSAELERLRQQVDED
                     RRAAEARIESDRQARIAAEHQLDETFSRYEGSIATSSPSDVDAMRRQIEDQQLALRTI
                     QERERANAEAMQAEITRLHTDLTASQQAGTLPPDVLSQRQADIVARQQQLDLLRQSLE
                     REAETRRQLADQQQQAIAAAEARRKQTDAEAAALRQQAENATQQAQAAQAEAQTANAQ
                     AQQANAQAQQAQAAAQQAQSQLEETRRELAERDAETRRLRIQNELNRIAATRSDPRGL
                     IVTLSGGILFDTGKTALKPGAKATLKRIAAQIATDPSLKIAVEGHTDNVGGKDTNQAL
                     SEKRADAVRDYLVSAGIPSDHISASGKGEESPIVTNKTAAGRQQNRRVELVITQ"
     gene            complement(9960..10670)
                     /locus_tag="VNN25_20530"
     CDS             complement(9960..10670)
                     /locus_tag="VNN25_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93978.1"
                     /translation="MTALLSVHNLEVFYGNVRALRGIDLEVRKGEIVTLIGANGAGKT
                     TTLRTISGLLKPKTGEVTYDGRTITGTKPHVITAMGVSHVPEGRGIFANLTVNDNLEL
                     GAYLRKDKIKQSEYDRIFTLFPVLKERIKQSAGTLSGGEQQMLAISRALMSKPQVLLL
                     DEPSLGLAPQMVQTIFRVIKEINAEGTTILLVEQNAHMALVTAHRGYVMETGRIVLAD
                     ETKALLSSDRIKKAYLGG"
     gene            complement(10667..11458)
                     /locus_tag="VNN25_20535"
     CDS             complement(10667..11458)
                     /locus_tag="VNN25_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH93979.1"
                     /translation="MANGNALLDARDVTIRFGGLTAVSHFNLAIQPHELVGLIGPNGA
                     GKTTVFNMLTGVYKPTEGEIFVGGVSTHEFKPYQITALGVARTFQNIRLFKELSVIDN
                     VKIGGHIHYKYSGTAAVLKTNRFHDAEEVADRDATELLRIFGLEKRADYYAKNLPYGD
                     QRRLEIARALAARPKLLLLDEPAAGLNDRETRDLMQTIHDIRDRFDIAILVIEHNMEL
                     VMGICERIIVLSFGKTIAQGSAAEVRANPVVIEAYLGEPAEAAGL"
     gene            complement(11458..12546)
                     /locus_tag="VNN25_20540"
     CDS             complement(11458..12546)
                     /locus_tag="VNN25_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXH93980.1"
                     /translation="MKPSLPWWLRDFLLLSGFVAVLIGLSYGLEAIPIAGIAYYNRIL
                     VIMGINITLAVSLNIINGHAGQFSLGHAGFMAVGAYFSAYLTYYHFAPRLDKMPAGTQ
                     QFVMQNVYLVVAVLAGGLLAALAGYLVGLPSLRLRGDYLAIVTLGFGEIIRVLILNID
                     TIGAARGFSGIPHWGNFFWVFLVAGLTIVISWRLVQSSVGRAFLAVREDQIAAEAMGV
                     DTTKYKVKAFVIGSFMAGVAGGLYAHYTMYLNPQTFMFVKSFEVVAMVVLGGLGSISG
                     SVVAAIILTFLPEGLRSVKDYMPGGRDPRMVIYSIMLIVLMLTRPQGIFGRRELWQIF
                     GRRKKTDVTPDAGDSNDKVSGDVAPPIQ"
BASE COUNT         2267 a         3993 c         4022 g         2310 t
ORIGIN      
        1 ccgccgcaat tccgacgagc gtgaaggctc catccgaatc ggccgttgca ttgcgcggcg
       61 cgatcggaga gcgcggcaac tcgacgatgg cgccggcgac tggcgtgccg tcggcgtgaa
      121 caacgcgccc gctgatcgtg acaccgcgat cgacttcgac cacgagcggc tccgagcgcg
      181 cggtgagcgt gacgctcggc acgcgtttcg gcgcaacctc gtcgccggtg acgaagacgg
      241 agtgcgggcc ctcggatgtc cggaagacga ccgagccatc agcactggtg cgtcggcaat
      301 cctgacgcgt cggatcatca cacgctgcag gagcgaaaaa gccctgttgc gcaccgtcgc
      361 cggtgcgtgt cagattcacg tacgcgtgtt ccaccggttg atgctgccgg tcgcggacgc
      421 ggacggcaat cgcgaatccg cgcaccagcg tgatgacgat gtcgtttcgc gtttcgccgg
      481 gctgccactt gcgcgcttcc agcatcccgc ccgcgtaccc gcgtttcgac gcgtaaatcg
      541 gcagcgcgaa cggcgtcgcc gaggtcacca cgcgaatcgc gaactcgccg gcggcattcg
      601 tgagggtgct cggccgccgc ggaccggtgg tcacgggcga ggtcacgatc gcgccggcaa
      661 ccggtttttt ctcttcgtcg accactctgc cgcgcgcatg ggcaaccggg agcgccgcga
      721 acgcgcgcgt cgtaacatgc ggcacaagga ccgcggcgga cgcgatcccg tacgatgggt
      781 gcattccttc gatctgatac gtgcgcgaaa ggagcgggcc aaaggtgaac ctcccctttg
      841 cgtcgctgac cgcagtcgcg ggaagcgagt cggcgccttc attcaatgac atccgcgcgc
      901 ccgccactgc agcgccggtc acggagtcgc gcacggtgcc cgtgaacgtc tcgcctttgg
      961 ccagacgaat ctcgaccgcg cccgttttcg ccggtcgcgt agccgcaccg atctcgtttc
     1021 cgcgcgccgc cgtcagcaac tgccacccgt caggcgcatg cgcgacgacg aaggtgccat
     1081 catccgccga ttccgcaagc ggccagccgt tgacggaaac gatcgcatgc ggcaccggtc
     1141 ccgcaccgtt cacgaccctg ccgcgaacgc tcacaccccg ggagagtttg atctcgtgga
     1201 gcgacgagat cgcgccgttc atgtcgcggc gcgatccagg cgcaaagtcg ggatggacga
     1261 cgtagaccga gccgatgccg aagactggag tctcgccggc gtcgtctgtc tttctcacct
     1321 cgctcccgga gaggacgatc gcgtttgcga ccggcttgcc gccgctcgtc acgcgaatct
     1381 tcgctaccgg tggtgcggcg agctgaatga cgccaatgtc gtcgccgtcg acggtggcga
     1441 tggtcgtgcg tgggtgatcg ggtgcctcga tctcgaggtc gacggccggc gatgacttca
     1501 ggtcgatcga gaaggtgccg ctatccgacg acttggccgt tgcgcccggg tcgcgatcgg
     1561 ccttccccgc cactagacgc gcgcgcagcg cggcagagct ctccggagca aaggcgcgga
     1621 tggtggcacc ggcgaccggc ttcccatcca tatcgatgat cgtgccggta atcgcggcat
     1681 gggcgatggt cgatgacgac gtgacgaaaa gagcggcgag agcgaagcgg gcgagtttgc
     1741 gcatatctat aagacgaatc atcatgaaag acgtttctca atggcggtgt gtccctgggc
     1801 agcggcgagg tcggctggcg ttttgccgtc gtcgccggca gcgcgcggat cggctccggc
     1861 agcgagaaga aggtcgagca ttacatcgtc tccatgttgt gcggccgtat gaagcgccgt
     1921 gtagcccatc tgctgccgcg cgttcacgtc ggctccgtgt ttgatgagta gttgcatgat
     1981 cgccgggtcg cgcaccgcaa ccgccgcgtg gaccggtgcg acgcggaacg cgttgcgcgc
     2041 tgcggcgttg acgtcggccc cgcgctcgat gagggtgcgc gccagctcgg ggtgacgaaa
     2101 aaagaccgcc aagccgatcg ctgggaatcc atcgtccgag taggaatcga gcagcgcgga
     2161 gtctgcgtcc agcatctggg ctgccagttc ggtgcgtccg agcgcgcacg cttcgccgaa
     2221 cgtgagcgtg gcgccgcggt ccaggaaaag ctgcgcgagc tctgcatgcc cgttgtaggc
     2281 cgcgaagaga atcggcgtga tgccgttctg cctggcaccg atgagcgcgg gctcggcgtc
     2341 gagaaggcgg gccacggccg cggcatcgtt ctcgcggata gcgtcgaaga gcgctttgtc
     2401 ctgcatgagg tgaaatcgta atccacaccg gggagagaac aagcgcccga aaggcccttt
     2461 tttagccatt ttctccacca tttgcagact cttgtatttc cagcagaccg tgcagggaca
     2521 ataacggcac ggagcttgat ctgaatgagt gcgatggaca cgaggaaatg cgagatctgc
     2581 ggccattcgc gtgcggcgca cgtcgacggc gtccactgct ccctttgccg ctgcaactcc
     2641 aagcgcacgg tagccgtgca ggaatcgctc gccttccgaa gctccctccc ggtccgcacg
     2701 gccggaatcg gcaggaagcg ataatggaag cgcaggatta cttgacctga tcccgccgtt
     2761 gcaaattgtc atgagcgaaa agaccgcgaa agtctccaag accgtcaccg tgtctgaacg
     2821 caacctgcaa agcgccgcgc tccgtctgtt gccgaaaaac aacaagctcg tttcgcccga
     2881 ggtcgactac ctccgccggg tactgggcga acgagccacg cagcagcaga tcgacgagaa
     2941 ggtccagcag gtgcgcaagc tcccctggtc cgaaatcgtg cacgagtgag tgcgacgctt
     3001 tccctgcgct tgatcgatga agaacgatga aatgacatct gcggacgaag ctccgcagat
     3061 cgagacagtt gatcccgatc tcccgggtac aactgatatt ccgatccgac ccgaaatccg
     3121 cgcggcattc gagcctactt gctgcggatc gggctgtgcg gattgcccgt tttgatgttg
     3181 aaccacagag acacagaggt cacagagaaa ggcagtaact ctgtgacctc tgtgtctccg
     3241 tggtgatcgg ttttagatac ccatcgagtt cgcatacaac ccatgaaaat gatggacgcc
     3301 gttctcgcgg gacggcgagt accggccgtt gtcgtaggtc gatgaggcca atccacgctg
     3361 cacggcttcg cagatgtcga tgtcctcgag ctggatctcg tcgctgaacg cgaccgtctc
     3421 gtcgatgcgt tgccgttcgg cgggcgcgtc gggatcgcgg aagtaccact cgaacagcgt
     3481 cagcgtcctc gtcgggccga gggggatgat gaggttcgtg gaaaagttgt cgggatagac
     3541 gttcagcatc aggttcggcc agacccagaa gtaatcgaca tcgtctcccg cgtcgttcac
     3601 gcggatacgg tcggggcgtt tgagcggcgc gacctggatc gagtagttgt ccctcgtctc
     3661 cgtgcggtaa ttcggatagt cgatctcgcg gaagagaccg ggatggacga tggggatgtg
     3721 gtacccctcg agatagttgt cgacgtagac cttccagttg cactccagcg accactcttt
     3781 ccgcgcagcg agccggtacc cttcgtagtt ccgcggcgcg agccggctga cgagatcgcc
     3841 gagaaactcg gtcagcggct cggcgtctgt gtcgaggttc acgaagacca actcgttcca
     3901 gacttcgacg cggaatcgcg gcagcgagaa atcgtcacgc tcgaagcatt cgacgccgct
     3961 catctccgac gtctgcagca accggccgtc aagcgcgtac gtccagccgt gatagccgca
     4021 ctgcagaacg ggacgtttgc cctccccttt cgcgaccgga ccggcgcgat ggcggcagac
     4081 gttggacatc gcgcgcagca cgccgtcgct ccctaggacg accagcaccg gctcgttggc
     4141 gatcgtcgtc gtgaagaact gtcccggatc gcggacgcga tcggcgtgtc ccacgagttg
     4201 ccatgtccgg ccgaagacgt tgcggttctc ggcatcgagc acatcccgat cgaagtagta
     4261 gcgcgatggg agcgtccgcg cgaaggcaag gcggggatcg aactggtagt tggtcatttt
     4321 cgtcggacga gaaggtacaa cgggatcgtc agaatgagga cgccgagggc gtagagcgag
     4381 ttgcgcgtgt cgctgatgac ggcgccgatc agaaacgcga tcgaggcgag gaatgccaga
     4441 ccggtagtga acggatgacc gatcgcccgg aacgggcgcg gcgcatccgg cgagctgcgg
     4501 cgcatggcaa agagcgacgc gaacgaaagc gcgtagttgg ccacgaagaa gaacgccaga
     4561 atcgcgatca cctgctcgaa cttgcctgag atcacgaaca ccgccgcgag gatgctgctg
     4621 aacagcaacg cgatgtccgg cgtccccccc ttattcacct cagcagcacc acgccagaac
     4681 agcttgtcac ggctcatcgc gaagagcacg cgcggcgcaa tcagcacgtt cgaattcacc
     4741 gcgctgatca acgagacgat catgatgatg cgaacgatgg tgtcgccgta cgcgccgaac
     4801 agatgctgcg ccacgacacc cgccgcgagc ttctgcccgg ccagactgcc tagtgggacg
     4861 acgcggagaa acgcgatatt gacgagcaag tagatcgcca tcaccgcaaa cacgccgccg
     4921 aacatcgccc gcggaatgtt gcggccggag tccttcacct cttccgaaaa atagatcacc
     4981 gccgtccatc cgtcgtaggt gtagatgacg gcctgcagag cgaggatgaa agcgatagcg
     5041 agagatcctt cgtgatccgc ttcgatgagc gatgccgtcg ggtgccgcgt gccgaggtag
     5101 aagcaggcgc cgatgaggaa gaggaaggcc agcgctttca aaaggctcgt cgcatcctgc
     5161 gtccagccac cccagcggac gccgaaccac tggatcagag tcaaaatggc gatgacggtg
     5221 agtgcagtcg gctcgatgcc gcgcagcgca ggaaacagga cgagcgcgta ctcgccgacg
     5281 acgatggaga cggcggcggt cgtcccgcag gtcgagatcc agtcgctcca gccgacgacg
     5341 agaccggcgt aatcgccgag cgcgcgccgc acgaaaacgt attgcccgcc ggattccggc
     5401 acgagcgtac ccagctcagc cagcgcgttc gcgccgagaa gcgcgtagag gccgccgatg
     5461 acccagacgg aaaggaaggg ccagaaggaa gggagctgcg tggcgatgtc gccgggcgtg
     5521 cggagaatcc ccgcaccgat catgttgccc acggtgacgg cgaggccgaa cgtcacgccg
     5581 aggacggtga gaagtttcgc gcgcggtggc aaggcggggg aagtgtaacg tgctggtgcg
     5641 gtctcacgct ttttcagcga cacctgtgcc tcgagcgcct tctcccctcg tgcttttcga
     5701 ggggagaagg tgccgaaggc ggatgagggg tgtcgtgcaa tcgggacgga ctgcgaacta
     5761 atccgcttcc tttaccgaca ccgggccgtt gtgcgtcgcc agtgacacgg cgcgcgtgcc
     5821 ggaaccgagc tcgatgcggc gcggccagcg cgggccgaaa tcgttgtcat cctcgctgct
     5881 cgcgtacgct ttcttcgcgg ctgcacagcc ggcggcgcgg caggacacgg gcgagttgcc
     5941 gtcggtctcg acgagcacac ccgagcgata gccgctgggg agcttgagcg acatcgggcc
     6001 gttctccgtc gaggcatcga ggcttccggc tccccacgag ccgccggtga gcttcacgga
     6061 gatgggaccg ttggtcgcgc gcagcttcac attgccggag gcgccggcga aggagatcgg
     6121 cccgttctgc gtgttgatat cgagtgtgcc cgacgaggcc ttcaccgcga tcggaccgtt
     6181 gagggcgtgc gcggtgatct ctccgtcgac gccgtcgatg gagatcggcc cattgctcgc
     6241 gtcgaggtcg atcgcagcac ctcggggcgc gaagacgagt aagtagatga tcgagcggcc
     6301 gtcggtctcg gcccgatcgg cgctcacctc attgccgctg acgttcacgc gcaagtcgtt
     6361 cacattccca agcgcagagg ccttgcacgc cttgaccgca aaactcgagt tagtcgacgc
     6421 gagaacccga atggagccgt tgcggtcgct gcggactttg agcgagcgca ggctgccgac
     6481 cggaacattc tcttcgacga ccggcacaga ccgatcgttg taggtgacgt gcagcgcact
     6541 gcaatcgcga atgtcctccc agtcatcggt cgtgaccgaa agtccgtggt tcgagtaggt
     6601 gctgtgcgag gaatgcgaga cggcagcaag gggcagcgtg agggcaatga gggcgggcag
     6661 aaaagcgagt cggcgcatgg taatcctccg ttctggtggg ttggacgacg cgatgggacg
     6721 aaagttagct catatccgca gatgacgcag atgagacgca gatagaggcg ccgctcgtac
     6781 aatccgcgtt gatgccgggc tttctcaaac gtgctgcgcg ccgcctcccg cttcttcgcg
     6841 gtcttcatcg ctacatcctc gcgctcgaag ccgagcgcga tcagttgcgg gccgatctgg
     6901 cgcactggaa gacgtggatg tcgcccggac acttctactc gcccattcca tcactcgacg
     6961 agatccggtc acgcgagtcc gagatcttcg cgccggcgcc gcctgcactg cctggcatcg
     7021 acctgcgcga aaaggatcag gtggcactcg tacgcgagct ggcggcgttc tatcccgctc
     7081 ttcccttccc ccgccagcaa tcgccatcca cccgctactg gttcgagaac tgggcgtttt
     7141 cgtacgcgga tgcgatcttt ctctattcga tgctccgcca tctccgcccg catcgcgtca
     7201 tcgaagccgg ctccggcttc tcctcggcgg ccatgctcga cacggccgac cgctggttgc
     7261 cggacacgtc gttcaccttc atcgaccccg acacagcgac gctcgacgca ctcctgcggc
     7321 cgggcgaccg ggagcgcgtg acgatcctcc cgctgcgcct ccaggacgtt cctctgacca
     7381 cattcgacgc actgtccgcg aacgacatcc ttttcgtcga ctcgacgcat gtctcgaaga
     7441 ccggtagcga cgtcaaccgc atcctcttcg acatcctccc ccgcctcgcc tccggcgtgt
     7501 acatccactt ccacgacgtc ttctatccgt ttgaatatcc gaaggagtgg gtctacgaag
     7561 gtcgcgcatg gaacgaggac tacatcctgc gcgccttcct cgagttcaac gactccttcg
     7621 aaatcgtcct cttcggcacg tggctggcgc gcttccaccg cgagctactc gaccagcaga
     7681 tgccgctgac gcgcgagaat cccggcggga gtctgtggct acgccggaag tagcgccgtc
     7741 ggctcgacgg ctgtaccgcc agcggctcgc tggcgacctc acacgcaaaa ccgccgccgg
     7801 gccggcggcg aaccagccgg cgaggccgcc ggcgctacga tcgcgccacg attcactgcg
     7861 taatcaccaa ctccaccctc cgattctgtt gccggcccgc cgcggtcttg ttcgtcacga
     7921 tcggcgactc ctctcctttg ccgctggcgc tgatgtgatc cgacggaatg ccggcggaca
     7981 cgagatagtc gcgcacggcg tcggcgcgtt tctcggacag ggcctgattc gtatcctttc
     8041 cgccgacgtt gtcggtgtgg ccttctacgg caatcttcag cgacggatcg gtggcgatct
     8101 gcgcggcgat ccgtttgagc gttgccttcg ctcccggctt gagggcggtc tttccggtat
     8161 cgaaaaggat gccgccgctg agcgtgacaa tgagtccccg aggatcgctt cgcgtcgccg
     8221 cgatccgatt cagctcgttc tgaatccgca gccgcctcgt ctcggcatcg cgttcggcga
     8281 gctctctcct tgtttcctcg agctgactct gcgcctgttg cgccgcagcc tgtgcctgct
     8341 gcgcctgcgc attcgcctgc tgtgcctgcg cgttcgccgt ctgcgcctcg gcctgcgcgg
     8401 cctgcgcctg ttgtgtcgcg ttctcggcct gctgtctcag cgcggcagcc tccgcgtctg
     8461 tctgtttgcg gcgcgcttcg gcggccgcaa tcgcctgttg ctgttgatcg gcgagttgcc
     8521 gacgcgtttc ggcctcgcgc tccagtgact ggcgcagcag atcgagttgc tgctgccgcg
     8581 cgacgatgtc cgcctggcgt tgcgacagga cgtccggagg aagcgttccg gcctgctgcg
     8641 atgccgtgag atccgtatgc agccgggtga tctccgcctg catcgcctcg gcattggcgc
     8701 gctcgcgctc ctgaatcgtt cgcaacgcca attgctgatc ctcgatctgc cggcgcatcg
     8761 catcgacgtc cgacggactt gatgtggcga tcgatccttc gtacctgctg aaggtttcat
     8821 cgagctgatg ctcggcggcg atgcgcgcct ggcggtcgga ttcgatcctg gcctcggccg
     8881 cgcgccggtc ctcatcgacc tgctgacgca gccgttccag ctcggccgac tgcgcctggc
     8941 gattggcctg ttctgctgcg agctgctgtt gcatcgcagc cgtgcgccgt tccgcctctt
     9001 cacgctgcgc gcggtccgcg gcagcctgcc gctcggccat ctgttgcgcc aggcgcgtgc
     9061 gatcgagttg aacggacggg agaaacttgc tcgattgcgc caggcgcgca aggtagaacg
     9121 cgcggcgtcc aagcatctcc gcgcgaaatg ccaggtcgtc gccgggctcg ctctcgcttg
     9181 tgtttcgtga gacgcgtttc gcgctatcga tgtacccctc ggcaatcctg atgtcgttgt
     9241 ccggaaccgt gtccagtgca ccagccacct tggcctgatc gaccgcgaac tgtgccgccg
     9301 cgatctgttc cttcgtggtc ccgccgcgat ggaactcgac catcggcgac tcctcgggaa
     9361 gaaggattac gggcgacgtg ccgccgaacc ggctgatgtc gctctgcaga ccccgaatcg
     9421 cgctgttcgt gctcagccag cgtgctttgg ccgccgcggc ctgcgcctcg accgtcgcct
     9481 cgatcgcgct catttgcgcc tgcccccgaa tcgccggctt cgacgagttc cagttctgtt
     9541 ccgcaaaccg cgcgcggtag acagcgtcgt catagagagt cttggcaaac gccggcgcgc
     9601 cagcggaatc cgccgcggcc aacgcctgct gcgccatatt cagatcggtt gccggctggg
     9661 ccaccagcgc cgtcgtgacc aacacagcga gcacggccgt tcgtaatgcg tacatggtgc
     9721 ctcctcgttt cagcgagagg agtgcaaggg tgatgcgcga aagtgcgaca agaaccaccg
     9781 acgggcaggg acgcccgtcg gcagcaggtg tggacacctg cactacttgg ccggcgattc
     9841 tgtccgggta cgttgcgacc gtagtgcagg cattcttgcc tgcaaccggc gggcgtcctc
     9901 gcccggcggt ggtcctcgaa atggaggcgg ctcccgatga gcaggagtga agtgccgcac
     9961 tacccgccga ggtacgcttt cttgatccga tcgctcgaga gcagcgcttt cgtctcgtcg
    10021 gctagcacga tgcggccggt ctccatcacg tagccgcggt gggcggtgac gagggccatg
    10081 tgggcattct gctcgaccag gaggatcgtc gttccttctg cgttgatctc tttgatcacg
    10141 cggaagatcg tctgcaccat ctgcggggca aggccgaggg agggctcgtc gaggaggagg
    10201 acctggggct tcgacatcaa ggcgcgggag atcgccagca tctgctgctc gccgccggac
    10261 agcgttcccg cgctttgctt gatccgctct ttgagcacgg ggaagagcgt gaagatccgg
    10321 tcgtactccg actgcttgat cttgtccttg cgcagatacg cgccgagttc gaggttgtcg
    10381 ttcaccgtca ggttcgcgaa gatcccccgc ccctcgggta catgcgatac gcccatcgcc
    10441 gtgatcacgt gtggctttgt cccggtgatc gtgcggccgt cgtaggtcac ttcgcctgtt
    10501 ttcggtttga gcagtccgga gatcgtacgc aacgtcgtcg tcttgccggc gccgtttgca
    10561 ccgatgagcg tcacgatctc gcccttgcgg acttcgagat cgatgccacg cagcgcccga
    10621 acgttgccgt aaaacacctc gaggttgtgg acgctgagga gcgcggtcat aggcctgccg
    10681 cctccgcagg ttcgccaagg tacgcttcga tcacgaccgg attcgcgcgc acctcggcgg
    10741 cgcttccctg cgcgatcgtc ttgccgaagc tgaggacgat gatgcgctcg cagatgccca
    10801 tcaccagttc catgttgtgc tcgatgacga ggatggcgat gtcgaaacgg tcgcggatgt
    10861 catgaatggt ctgcatcagg tcgcgggtct cgcggtcgtt cagacccgcg gccggctcgt
    10921 cgaggagaag cagcttcggg cgcgccgcca gggcgcgcgc gatctcgaga cggcgctggt
    10981 cgccataggg cagattcttc gcgtagtagt cggctcgctt ttccagtccg aaaatgcgca
    11041 gcagttccgt ggcgtcgcgg tccgcgacct cctcggcatc atgaaaccga ttcgtcttaa
    11101 gaaccgcggc ggtgccggag tatttgtagt ggatatggcc gccgatcttc acgttgtcga
    11161 tcaccgacag ctctttgaag agccggatgt tctgaaacgt gcgggccacg ccgagcgcag
    11221 tgatctggta cggcttgaat tcgtgcgtcg acacaccccc gacgaagatc tctccctcgg
    11281 tcggcttgta gacgccggtg agcatgttga agaccgtcgt ctttcccgcg ccgttcggac
    11341 cgatcagtcc caccagttcg tgcggctgaa tggccagatt gaaatgcgac accgccgtca
    11401 gaccaccgaa gcggatggtc acgtcgcggg catcgagcag agcgtttccg tttgccatca
    11461 ctgaatgggt ggagcgacgt cgccgctcac cttgtcgtta gaatcacccg catcgggagt
    11521 gacatccgtt ttcttgcgtc tcccgaaaat ctgccacagt tcgcgccgtc cgaagatccc
    11581 ttgcggccgg gtcagcatca gcacgatgag catgatcgag tagatgacca tgcgaggatc
    11641 gcggccgccc ggcatgtaat ccttcaccga gcgaagcccc tcggggagga acgtcaggat
    11701 gatcgctgcg acgacggagc ctgagatcga gcccagtccg cccaggacga ccatcgccac
    11761 gacctcgaac gacttcacga acatgaatgt ctgcggattg aggtacatcg tgtagtgcgc
    11821 gtagagcccg cccgcgactc cggccatgaa cgagccgatc acgaacgcct tgaccttgta
    11881 tttcgtcgtg tcgacaccca tcgcctcggc ggcaatctga tcctcgcgaa ccgccagaaa
    11941 tgcccggccg acggatgact gcacgagccg ccacgagatg acgatcgtca atccggccac
    12001 gaggaagacc cagaagaagt tgccccagtg cggaatcccg ctgaacccgc gcgcggcgcc
    12061 gatcgtgtcg atgttcagga tgagaacgcg gatgatctcg ccgaatccga gcgtgacgat
    12121 ggcgaggtag tcaccgcgca atcgcagcga cggcaagccg acgaggtagc ccgccagtgc
    12181 cgcgagcagt ccacccgcca gcaccgcgac gacgaggtag acgttctgca tcacgaactg
    12241 ctgcgtaccc gcaggcatct tgtccagccg cggagcgaag tggtagtaag tcagataggc
    12301 ggaaaagtat gcaccgaccg ccatgaagcc ggcgtgcccg agggagaact ggcctgcgtg
    12361 tccgttgatg atgttgagcg ataccgccag cgtaatgttg atccccatga tcacgaggat
    12421 ccggttgtaa tacgcgatgc cggcgatagg gatcgcttcg aggccgtacg acaatccgat
    12481 caacaccgcc acgaagccgg agaggagaag gaagtcgcga agccaccagg ggagagacgg
    12541 tttcacgcga ccctcttgag aaaactagaa ggcagaaagc agaaggcaga ag
//
LOCUS       DATLVW010000707         6300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_156083, whole genome shotgun sequence.
ACCESSION   DATLVW010000707 DATLVW010000000
VERSION     DATLVW010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156083"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..930
                     /locus_tag="VNN25_20545"
     CDS             223..930
                     /locus_tag="VNN25_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93981.1"
                     /translation="MTIEKLLADREASGLNIADKLQQLFRYGAPLVQLHVEAGEDKAP
                     SIRYFGTANGDSPLGDIVKRIGQEARQYRSGVETEITVVTIKAGFPAHHVALLVHCDF
                     VIEQQKDRARFQVVADWKLEPLIPSVLMLGNAEDTARQLACESLALRVVTEEPEGLVM
                     DGAVIGPSYRAVIELLRSLRGSGLKAKLRLKTTERLAAKGAAEMLSSFLTKGAGDAID
                     QSIIQSALDRLDESASP"
     gene            complement(922..1638)
                     /locus_tag="VNN25_20550"
     CDS             complement(922..1638)
                     /locus_tag="VNN25_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93982.1"
                     /translation="MLAAHLIRLLPGLLALLIVGLALMKSSDRLHPLILWLAVVTAGA
                     PLAIGYVWARSPIEGLLYGMLTYAAACGFAIRLVRHDLPFRYHLPLLILFMASAVTAF
                     VTIPLVVMPSVRDMTVAVDTEEGTPDLKIQQALRDVDSALVNIESEHVKLQRGVRDVM
                     EHVKMRSARIRELQDQRDKVMEDLRRLEPLRQLTVVQIAALTAQLHKTQVSDNIISFF
                     IGIASSITATFAWKFFGQSR"
     gene            complement(1676..2155)
                     /locus_tag="VNN25_20555"
     CDS             complement(1676..2155)
                     /locus_tag="VNN25_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HXH93983.1"
                     /translation="MWNIALPWWEFVVRGVIVYGFLIVLLRVTGKRQVGQLAPFDLVL
                     LLVLSNAVQNSMNGGDNSVVGGLISAATLVGVNYFVGFAAFRSKRLERIIEGRPEVLI
                     HNGILYKDVMARAQLTHHELDAALRRNGCLTVEEVHSAILENNGSISVTRRVNHTPV"
     gene            complement(2271..3302)
                     /locus_tag="VNN25_20560"
     CDS             complement(2271..3302)
                     /locus_tag="VNN25_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXH93984.1"
                     /translation="MLNRRDFAKLSVTAAAAVATTRTLNAATPTIVKPKKLSPGDTVG
                     LVLPATAAFEADEIQFAKEQMEAIGFKVVIGKHAFDKWGYFAGHDRDRADDINQMFAD
                     DSVAGVVCYTGGWGSPRVLPYLDYDLIRRKPKVLIGYSDITALLNAVHKKTGLITFHG
                     PVGASTYDPFTLENFRKVVMTAEPAGILPTPSKKPNELVDRTNRVLKISPGKATGPLI
                     GGNLTMIATLMGTPYQPDTTGAIVFLEDVHEEPYRIDRMLTTLALGGMFDKAAGIVFG
                     RCSDCGVKGPSLSLEEILRDRFGGLEVPVISGLSFGHIEQKLVLPIGARATLDADGGT
                     LRMEEGAVG"
     gene            complement(3455..>6300)
                     /locus_tag="VNN25_20565"
     CDS             complement(3455..>6300)
                     /locus_tag="VNN25_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase"
                     /protein_id="HXH93985.1"
                     /translation="WTRNSVSYGNGIYKSTDGGDNWTNVGLPNSERIAKIAIDPTDTN
                     TVYVCVPGKLWSDSEDRGVYKTSDGGKTWTNVLKGANLSTGCSMLSLDRANPKTLFAG
                     MWDFRRKGWTFRSGGDGADAPSGSGLFKSTDGGATWQSLDDKTAAGLPKKPWGRIAVT
                     VAPSNSNVVYALVEAAPPTNGLYRSEDGGKTWQARDRSQMMIWRPFYFANLLVDPKDA
                     NKLYKAGGGLIASNDGGKSFSGVGGAGHGDWHDVWIDPNNTDNLIAGDDGGLWYSYDG
                     GNRVWKADNLPVSQFYHVSVDMDRPYHVYGGLQDNSSWVGDSQYPGGITSSRWENFYG
                     GDGFWMFADPSDPNYIYAEAQGGEIGRINRKTHETRPIKPLPQYKEGKLRFNWNTPIH
                     VSPKGTLYIGAQFLFRSNDHGQTWERISPDLTTNDPAKQKQEQSGGVTVDNSSAEMHT
                     TIFAIAESLKNSNVIWVGTDDGNVQVTRDAGKTWTKVTPNITGLPKNAWVTSVEPGHF
                     DEGTAYATFDMHTFGDMQPYAYKTTDFGKTWTSVLASDAPVHGFAHVVKEDLVNPNLL
                     FLGTELGLWVSLDGGKQWAQYKGGDMPNVAVDDLAIHPRDNDLVIATHGRGIWIVDDI
                     TPLRALTPEVLAKDVVFLQGHPVVQTLSAQGGWANGDAAYVGQNPAGGAIITYYQRTR
                     HIFGDLSIDVFDANGKKMGNIPSSKRRGLNRVTWAMRLPAPKVPTAATAAFSASAGPR
                     VLPGTYTVKMTKDKNVYTTPLVLVPDPRATHTDADRQAQWDLSNKLYAQLGEMTFAVD
                     RINGVRTALDDRASKLPATDALAKKLRAASADVDTLRRKIVATKEGGMITGEERLREN
                     LTDLYGNVVFYEGRPSQSSVERTDALAHELKDVVQDFDAWTTKELPSINAALAKKKLD
                     PVTVITRADWDAKNGGASGAPAATERENFFERD"
BASE COUNT         1120 a         1801 c         2051 g         1328 t
ORIGIN      
        1 gtccaattcg cgcagacgct tttccgccgt tccgtgctcg tcccgggcga gatcgtgcac
       61 tttgtccagg gcgcagaacg gtatcaaaga gaagagaatc gcttccgaac ggctcatccg
      121 ctgtcgggag aattcgccgc attcgtcttg tcaggtggtt tggatcacct ggtgcagagc
      181 ttgcgcgacg catcggccga ggtattcgcg gacgtccaga agttgacgat cgagaagctg
      241 ctcgctgacc gcgaggccag cggcctgaac attgctgaca agctgcaaca gttgtttcgt
      301 tacggcgcgc cgttggtgca gcttcacgtc gaggccggcg aggacaaggc cccgtcgatc
      361 cggtacttcg gaaccgcgaa cggagactct ccgctcgggg acatcgtcaa acgcatcggc
      421 caagaagcac ggcagtatcg cagcggcgtc gagacggaga ttaccgtcgt gacaatcaag
      481 gccggcttcc ccgcgcacca cgtggccttg ctcgtccact gtgacttcgt cattgagcaa
      541 cagaaagacc gagcccggtt tcaggtcgtg gccgattgga agctggagcc gctcatccca
      601 tccgtgctca tgcttggcaa cgcggaagac accgcccggc agctcgcctg cgagtcactg
      661 gcgctccgcg tggtcactga agagccggag gggctggtta tggacggagc agtaatcggg
      721 ccgtcgtacc gcgccgtcat cgaactgttg cgatccctgc gcggttccgg tctcaaggct
      781 aagctacggc tgaagacgac cgagcggctg gcggcaaaag gcgccgcaga aatgctcagc
      841 agcttcctca ccaaaggcgc tggcgatgcg atcgatcaga gcattatcca aagcgctctg
      901 gaccgtctcg acgagtccgc gtcaccttga ttgcccgaag aatttccaag cgaaggtcgc
      961 tgtaattgac gaggcaatcc caataaaaaa cgaaattata ttgtcagaca cttgcgtttt
     1021 gtgaagttgg gcggtcagcg cagcgatctg cacaaccgtc aactggcgca aaggctccaa
     1081 ccgtcgaaga tcctccatga ccttgtcgcg ctggtcctgc aactcccgga tgcgagccga
     1141 gcgcattttt acgtgttcca tcacgtcccg gacgccccgt tgcagcttca catgttcgga
     1201 ctcgatattg actaatgcgg agtcgacatc gcgaagcgcc tgctgaatct tcaaatcggg
     1261 ggtgccttcc tcggtgtcga cggcaacagt catgtcgcgt acggatggca ttacgactag
     1321 aggaatggtg acgaatgccg tcaccgccga ggccatgaac aaaatcaaaa ggggcaggtg
     1381 gtagcggaag ggaaggtcgt gacgcaccag acggatggcg aagccgcagg ccgcggcgta
     1441 ggtgagcatt ccgtacagga ggccttcgat cgggctgcgt gcccagacat atccgatcgc
     1501 caaaggagcg cccgccgtca cgaccgctag ccacaaaatg agtggatgca accgatcact
     1561 gcttttcatc aacgcgagcc cgacgatcaa caatgccagt aaacccggga gcagcctgat
     1621 cagatgtgct gccaacattc ggaaccctcc tcgatcacta gtagcgaatt gtcatctata
     1681 ctggcgtatg gttcacacga cgcgtgacgc tgatcgatcc attgttctcc aggatggcgc
     1741 tatgcacttc ctccaccgtt agacacccgt tgcgcctcag ggcagcgtcc agttcgtggt
     1801 gcgtaagctg cgcgcgtgcc attacgtcct tgtaaagaat cccgttgtgg atgagtacct
     1861 cagggcgtcc ctcgatgatc cgctccagtc gtttacttcg aaatgcggcg aatcccacga
     1921 agtagttcac gccgactaga gtcgctgctg agatgagacc gccgaccact gagttgtcgc
     1981 cgccgttcat cgagttctga acagcatttg aaagcaccag cagcagcacg agatcgaacg
     2041 gagccaattg tccgacctgc ctctttccgg tgacgcgtag cagaacgatc aagaagccat
     2101 atacgatgac accgcggaca acgaattccc accaagggag cgcaatgttc cacatctgac
     2161 gattctacaa cgccttccgt actaccggca tgatgggaga tggtatctga agggcgaggc
     2221 gcactacttc gtttgagaag tggttgtcca caaatacaga taacaaatca ctaccccacc
     2281 gccccctcct ccatccttaa cgtcccccca tccgcgtcga gagtcgctct tgctccgatg
     2341 ggcaacacga gtttttgttc gatgtggccg aaggacaatc ctgagatgac gggtacctca
     2401 aggccaccaa acctatcccg caggatctcc tccagactga ggcttggccc ctttacgccg
     2461 cagtcgctgc aacggccgaa gacgatgccg gcggccttat cgaacatccc gccgagggcg
     2521 agggtggtta gcatgcggtc gatgcggtag ggctcttcgt ggacgtcttc gaggaagacg
     2581 atggcgcccg tcgtgtcggg ttggtagggg gtgcccatca gggtggcgat catcgtcagg
     2641 ttgccgccga tcagggggcc ggtggctttg ccgggcgaga tcttgaggac tcggttcgtg
     2701 cggtcgacta gctcgttggg tttcttcgat ggggttggga ggatgccggc tggttcggcg
     2761 gtcatcacga ccttgcggaa gttctcgagt gtgaacgggt cgtaggttga cgcgccgacg
     2821 gggccgtgga aggtgatgag gccggtcttt ttgtggacgg cgttcaggag cgcggtgatg
     2881 tcgctgtagc cgatgaggac tttcggtttg cggcggatga ggtcgtagtc gaggtagggg
     2941 aggactcttg gtgagcccca gccgccggta tagcagacga cgcccgctac gctgtcgtcg
     3001 gcgaacattt gattgatgtc gtcggcgcgg tcgcggtcgt ggccggcgaa gtagccccat
     3061 ttgtcgaagg cgtgtttgcc gatgacgact ttgaagccga tggcttccat ctgctctttg
     3121 gcgaactgga tttcgtcggc ttcgaaggcg gcggtagcgg ggaggaccag gcctactgtg
     3181 tcgcctgggc ttagtttctt cggcttgacg atggttggtg ttgccgcgtt gagggttctc
     3241 gtcgtggcga ctgctgcggc ggcggtgacg gagagtttgg cgaagtcgcg gcggttgagc
     3301 atggggttga agcttatccg cagatgacgc agatgggacg cagatagaga ctctgcggat
     3361 gagactcggc ccctcatccg gccttcggcc accttctccc catcaaaaag cgatggggag
     3421 aaggggctcg attgagaggt tttcgcgagt agccttagtc gcgttcgaag aagttttcgc
     3481 gctcggttgc ggcgggggcg cctgaggcgc caccgttctt ggcgtcccaa tccgctcgcg
     3541 tgataaccgt caccgggtcg agtttcttct tggccagggc ggcgttgatc gatggcagct
     3601 cctttgtcgt ccaggcgtcg aagtcctgca cgacatcctt cagctcatgc gccagggcgt
     3661 cagtgcgctc gacggacgat tgcgaggggc ggccttcgta gaagactacg ttgccgtaga
     3721 ggtccgtcag gttttcgcgg aggcgttctt cgccggtgat catgccgccc tctttcgtcg
     3781 ctacgatctt gcggcgcagc gtgtcgacgt cggcggatgc ggcgcggagc ttcttggcca
     3841 gcgcgtcggt tgcgggcagc ttcgatgcgc ggtcgtcgag ggcggtgcgg acgccgttga
     3901 tgcggtcgac cgcgaaggtc atctcgccga gttgcgcgta gagtttgttt gagaggtccc
     3961 actgcgcctg gcggtcggcg tcggtgtgtg tcgcgcgggg atcgggcacc aggacgagcg
     4021 gcgtcgtgta gacgttcttg tctttcgtca tcttcaccgt gtacgtgccg ggaagaactc
     4081 ttggtccagc cgaggcggag aaggcggcgg ttgcggcagt gggaactttg ggtgcgggaa
     4141 ggcgcatcgc ccacgtgacg cggttcagtc cgcggcgctt gctgctgggg atgttgccca
     4201 tcttcttgcc gttcgcatcg aacacgtcga tcgacaggtc gccgaagatg tggcgggtgc
     4261 gctggtagta cgtgatgatc gcgccgccgg ccggattctg tccgacgtac gcggcgtcgc
     4321 cgttggccca gccgccctgc gcggacaggg tctgcacgac aggatgcccc tgcaagaaca
     4381 ccacatcctt cgccagcacc tcgggagtca gagcccgaag cggggtgatg tcgtcgacga
     4441 tccagatgcc gcggccgtgc gtggcaatga cgagatcgtt gtcgcgcggg tgaatggcca
     4501 ggtcgtcgac ggccacgttc ggcatgtcgc cgcctttgta ctgcgcccac tgcttgccgc
     4561 catcgagcga gacccagagt ccgagctcgg tccccaggaa aaggagattg ggattgacga
     4621 gatcttcttt gacgacgtgc gcgaagccat gaaccggcgc gtcggaggcg aggaccgatg
     4681 tccacgtctt gccgaagtca gtcgtcttgt aggcgtacgg ctgcatgtcg ccgaacgtgt
     4741 gcatgtcgaa ggtggcgtaa gcggtgcctt catcgaaatg gccgggctcg accgacgtca
     4801 cccatgcgtt cttcgggagg ccggtgatgt tgggggtgac tttcgtccag gtcttgccgg
     4861 catcgcgcgt gacctggacg ttgccgtcgt cggtgcctac ccagatcacg ttgctgttct
     4921 tcagcgactc ggcgatggcg aagatcgtcg tgtgcatctc ggcggacgag ttgtcgaccg
     4981 tgacgccgcc ggactgttcc tgcttctgtt tcgcgggatc gttcgtggtc agatcgggcg
     5041 agatgcgctc ccacgtctgg ccgtgatcgt tcgagcggaa gaggaactgc gcgccaatgt
     5101 agagcgtgcc cttcggtgac acgtgaatgg gcgtgttcca gttgaagcgg agctttcctt
     5161 ctttgtattg cggcagcggt ttgatcggcc gcgtctcatg cgtcttgcgg ttgattcgtc
     5221 cgatctcgcc gccctgcgcc tcggcataga tgtagttcgg atcgctcgga tcggcgaaca
     5281 tccagaatcc gtcgccgccg tagaagtttt cccaacggct gctcgtgatg ccgcccgggt
     5341 attgcgaatc gccgacccac gagctgttgt cctgcaggcc gccgtagaca tggtacggcc
     5401 gatccatgtc gacgctgacg tggtagaact gcgagacagg gaggttgtcc gccttccaaa
     5461 cgcggttgcc gccgtcgtac gagtaccaga ggccgccgtc gtcgccggcg atcaggttgt
     5521 ctgtgttgtt cggatcgatc cagacgtcgt gccagtcacc gtgaccggcg ccgccgacgc
     5581 cgctgaagct cttgccgccg tcgttcgagg cgatgagtcc accgccggct ttgtagagtt
     5641 tgttcgcgtc tttcggatcg acgaggaggt tggcgaagta gaagggacgc cagatcatca
     5701 tctggctgcg gtcgcgggcc tgccatgtct tgcccccgtc ttcggagcga tagagaccgt
     5761 tggttggcgg cgctgcttcg acgagcgcgt agacgacgtt ggagttcgat ggcgcgacgg
     5821 tgacggcgat gcgtccccac ggtttcttcg gcaggcctgc tgccgttttg tcatcgagcg
     5881 actgccaggt ggcgccgccg tcggtcgatt tgaaaaggcc gctgccgctc ggcgcgtcgg
     5941 caccatcgcc gccggagcgg aaggtccagc ctttgcggcg gaagtcccac atgccggcga
     6001 agagggtctt cggattggcg cgatccaatg agagcatgga acagccggtg gagaggttgg
     6061 cgcctttcag cacattcgtc cacgtcttgc cgccgtcgga ggtcttgtag acgccgcggt
     6121 cctcgctgtc gctccacagt tttccgggaa cgcagacgta gacggtgttc gtgtcggtcg
     6181 gatcgatggc gatcttggcg atgcgctccg aattcgggag accgacgttg gtccagttgt
     6241 cgccgccgtc ggtcgatttg tagatgccgt tgccgtacga aacggagttc cgcgtccacg
//
LOCUS       DATLVW010000708         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_239527, whole genome shotgun sequence.
ACCESSION   DATLVW010000708 DATLVW010000000
VERSION     DATLVW010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239527"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1661
                     /locus_tag="VNN25_20570"
     CDS             9..1661
                     /locus_tag="VNN25_20570"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXH93986.1"
                     /translation="MATKFIFITGGVVSSLGKGVAASSVGAILEARGFSVTMMKFDPY
                     INVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFTSMRATRKSNFTTGRVYLKVIEK
                     ERRGDYLGKTVQVIPHITDEIKKCIREVSEGVDVVIVEIGGTVGDIESLPFLEAIRQF
                     RLEAGRGNAINVHLTLVPYIKASDEMKTKPTQHSVQKLREIGIQPDVLLCRSEHAIPD
                     EMRDKIALFCNVEQDAVIPALDVEFIYQVPLAFSRERLDEILLDKLHLPHDEIGALTK
                     WEEIIRKLRHPEDEVKIGFVGKYVEFGDSYKSLNEALTHGGIANNVKVRIIYIDSEEL
                     EAEGYHGELGRVDGILVGPGFGSRGVEGKLRAIRYAREKRIPYFGICLGMQCATIEFA
                     RNVVGMVGANSTEFDDESPYKVIYKLRDLIGVDALGGTMRLGKYPCRLKEGSIAHDAY
                     ANDLIDERHRHRYEVNPEYVPQLEERGFHVTGLSPDGKFVEIVELEGHPWFLGCQFHP
                     EYKSRPTAPHPLFRSFIAAARLFKNSAKATAAVSVAQETELV"
     gene            1658..2071
                     /locus_tag="VNN25_20575"
     CDS             1658..2071
                     /locus_tag="VNN25_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93987.1"
                     /translation="MKALRYVGGIIAGMIAAFALVASAEAIVHVLYPPPPGTNMDDFE
                     QVKRFIAALPVPAYLLVLTGWLIGTFVAAWLAAKIARDPFAGYIVGTLLLAAGIFNAI
                     KIPQPMWFSIVSFVIYIGATWVGARAGRTVSVATM"
     gene            complement(2074..2556)
                     /locus_tag="VNN25_20580"
     CDS             complement(2074..2556)
                     /locus_tag="VNN25_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93988.1"
                     /translation="MADELFDTLLRFHREVVQPELNEIRAEMRDMRSEMATRTELQAM
                     RAEMATRAELQAMRAEMVTWPVLMRFYNEVIKPESDHVRSLFESALRSEMLSHVDGIY
                     VKLNRLEIEYQALRAAVGRIEKELVVQNADRMEVRSELLGLKDQISKLNDRIAGRDIN
                     "
     gene            2609..3439
                     /gene="kdsA"
                     /locus_tag="VNN25_20585"
     CDS             2609..3439
                     /gene="kdsA"
                     /locus_tag="VNN25_20585"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXH93989.1"
                     /translation="MQTRPIAITDTISFGGGHPPLFIAGPCVIESREHALRMARTLLA
                     LRDELKINLVFKSSFDKANRTSIESFRGPGLEKGLEILRAVKGETGLPLLSDIHEWQQ
                     ADRAAEVLDIIQIPAFLCRQTDLIAAAARTGKAIGVKKGQFLSPEEAKNILDKGREVG
                     NERVFITERGSSFGYQNLVVDMRSFPILRGFGAPVVYDITHSMQKPGGEGKQTGGTPQ
                     FARPLARAAAAAGAEGFFMEVHDNPPAALSDRTTQILPEAAREIITDILAMRAALPEI
                     "
     gene            3454..>4361
                     /locus_tag="VNN25_20590"
     CDS             3454..>4361
                     /locus_tag="VNN25_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93990.1"
                     /translation="MSRLQRIIAAVIALYLLLVLLDGFVLVELLVRRTGLAVLLALAA
                     ALAIVGCGFALRALLARAWLPEECDIARDFLLGYPIFGTLCFLAGTLNVSSWSMATLL
                     IVCGVGGLYVVVRRLESRMPAMPALTEPLALIATAVVLLAALIAVQAPPTSLDELAYH
                     LAVPWAWVLNHRAIDLPLISHSYFPLGIESADLPLLTILGQIRGGIASHVLHLLAALA
                     TTAMLFRLARGNALAVAAIVTTPALALIAGWSLNDWPLIGICAVLVLALDQDDVPTWA
                     IALAAGMLTKYTFVPFAVIAVGVAMWR"
BASE COUNT          790 a         1407 c         1293 g          871 t
ORIGIN      
        1 cgaaacccat ggcgaccaag ttcatcttca tcaccggcgg cgtcgtttcc tccctgggca
       61 agggtgtcgc cgcctcctcc gtcggcgcga tcctggaagc gcggggtttt tcggtgacca
      121 tgatgaagtt cgatccctac atcaacgtcg atcccggcac gatgtcgccc ttccagcacg
      181 gcgaagtata cgtgaccgat gacggcgccg agaccgatct cgacctcggc cactacgagc
      241 gcttcacctc catgcgcgcc acgcggaaga gcaacttcac caccgggcgc gtctacctca
      301 aggtcatcga gaaggagcgg cgcggcgact acctcggaaa gactgtgcag gtcatcccgc
      361 acatcaccga cgagatcaag aagtgcatcc gcgaagtgag cgaaggcgtc gacgtcgtca
      421 tcgtcgagat cggcggcacg gtcggcgata tcgaatcgct ccccttcctc gaagccattc
      481 gccaattccg gctcgaagca ggacgcggca acgccatcaa cgtccatctc accctcgtcc
      541 cctacatcaa agcgtcggac gaaatgaaga ccaagccgac gcagcactcc gtgcagaaac
      601 tgcgcgagat cggcatccag cccgacgttc ttctctgccg ctccgagcat gccatccccg
      661 acgagatgcg cgacaagatc gccctcttct gcaacgtcga gcaggacgcc gtcatccccg
      721 cgctcgatgt cgagttcatt taccaggtgc cgctggcgtt ttcgcgcgag cgattggacg
      781 agatcctgct cgacaagctc catctcccgc acgacgagat cggcgcgctg acgaagtggg
      841 aagagatcat ccgcaaactt cgccatccgg aagacgaagt gaagatcggc ttcgtcggca
      901 agtacgtcga gttcggcgat tcgtacaagt cgctcaacga ggcgctgacg cacggtggca
      961 tcgccaacaa cgtgaaagtc cgcatcatct atatcgactc cgaggagttg gaggcggagg
     1021 ggtaccacgg agagctcggg cgggtggacg ggatcctcgt cgggcccgga ttcggctcgc
     1081 gcggcgtcga aggtaagttg cgcgcgatcc gctacgcgcg cgagaagcgc atcccctact
     1141 ttggcatctg cctcggcatg caatgcgcga cgatcgagtt cgcgcgaaac gtcgtcggaa
     1201 tggtcggcgc gaactcgacg gagttcgatg acgagtcgcc gtacaaagtc atctacaagc
     1261 tgcgcgatct catcggcgtc gacgctctcg gcgggactat gcgcctcgga aagtatccat
     1321 gccggctgaa ggaaggctca atcgctcacg atgcatacgc caacgatctg atagacgagc
     1381 gccatcgcca ccgctacgaa gtgaatcctg aatatgttcc gcaactcgag gagcgcgggt
     1441 tccatgtgac gggactctcg cccgacggaa agtttgtcga gatcgtcgaa ctggaaggcc
     1501 atccctggtt cctcggctgt cagttccatc cggagtacaa atcgcgtccg accgctccac
     1561 acccgctctt ccgctcgttc atcgccgcgg cacgcctctt caagaactcc gcgaaagcaa
     1621 ccgccgccgt gtcggtggcc caggagactg agctcgtatg aaagcgctgc ggtacgtcgg
     1681 gggaatcatc gccgggatga tcgctgcgtt tgcgctcgtc gccagcgcgg aagccatcgt
     1741 tcacgtgctc tatcccccgc ctccgggaac gaacatggac gacttcgagc aggtcaagcg
     1801 attcatcgcc gcgcttccgg tgcctgccta cctgctcgtg ctcactggct ggctgatcgg
     1861 aacgttcgtg gccgcgtggc tggcggcgaa gatcgcccgc gatccgttcg ccggctacat
     1921 cgtcggcacc ctgcttctcg ccgccggaat cttcaacgcg atcaagattc cgcagccgat
     1981 gtggttctcg atcgtctcgt tcgtgatcta catcggcgcg acatgggtgg gggcaagggc
     2041 ggggaggacg gtgagtgtcg cgaccatgta aggtcagttg atgtctcgtc ccgcgattcg
     2101 gtcattaagc ttcgagatct gatctttcag tcctaacagt tcggatcgga cctccatgcg
     2161 atcggcattc tgcaccacca actccttctc gatccggccg accgcggcgc gaagcgcttg
     2221 atactcgatc tcaaggcgat tcagcttgac gtagataccg tccacatggg aaagcatttc
     2281 cgaccgcaac gctgattcga aaagggagcg gacatggtca ctctcgggtt tgatcacctc
     2341 gttgtagaac cgcatcagaa cgggccacgt caccatctct gcacgcatgg cctgcaattc
     2401 cgcccgcgtg gccatttcgg cgcgcatggc ctgcaactcg gtacgcgtgg ccatttcgga
     2461 gcgcatgtcg cgcatttcgg cgcggatctc gttcagttcc ggttgcacga cttcgcggtg
     2521 aaagcgaagc agcgtatcga atagctcgtc ggccacggca cgattctacc atcccgtgag
     2581 tacaatcccg tgagtacaat gcccccgcat gcaaacgcgt cccatcgcca tcaccgacac
     2641 catctcgttc ggtggcggcc accctcccct tttcatcgct ggtccgtgcg tgatcgagtc
     2701 gcgcgagcat gcgcttcgca tggcccggac gcttctggcg ctacgcgatg agttgaaaat
     2761 caaccttgtt ttcaagtcat cgttcgacaa ggcaaaccgc acgtccatcg agtcgtttcg
     2821 cggtccgggc ctggagaaag ggttggagat cctgcgggct gtgaagggcg agactggatt
     2881 gcctctcctt tccgatattc acgaatggca gcaggcggat cgcgctgccg aggttttgga
     2941 catcatccag attccagcgt ttctctgccg gcagaccgac ctcatcgccg cggcggcgcg
     3001 taccggaaag gcgatcggcg tgaagaaggg gcagttcctt tcgccggagg aagcgaagaa
     3061 cattctcgac aagggacgcg aggttgggaa cgagcgcgtt ttcatcacgg agcgcggctc
     3121 atcgttcgga taccagaacc ttgtcgtcga catgcgctcc ttcccgattc tgcgtgggtt
     3181 cggtgcgccg gtcgtctacg acattacgca ctcgatgcag aagcccggcg gcgaagggaa
     3241 gcagaccggc ggtacgccgc agttcgcacg gccgctcgcg cgtgcggccg cagcggcggg
     3301 agcggagggg ttcttcatgg aggttcacga caacccgccg gcggcgctca gcgatcgcac
     3361 gacgcagatc ctcccggaag cggcgcgcga gatcatcacc gacatcctgg ccatgcgtgc
     3421 ggccttgccc gaaatctgat tcagcaaccg acgatgtcac gactccagcg catcatcgcc
     3481 gcggtcatcg cgctttacct gctgctcgtt cttctcgacg gcttcgtgct cgtcgagttg
     3541 ctcgtgcggc gtaccggtct ggcagtactg ctcgcgctcg ccgcagcgct ggccatcgtc
     3601 ggatgcggat tcgcacttcg cgctctgctc gcacgcgcgt ggcttcccga ggagtgcgac
     3661 atcgctcgtg attttctcct cggctatccg atcttcggca cgctttgctt tcttgccggg
     3721 acgttgaatg tctcctcctg gtcgatggca acgctgctga tcgtctgcgg cgtcggcggt
     3781 ctctacgtcg tcgtccggcg gctcgagtct cgaatgccgg ccatgcccgc actgaccgag
     3841 ccgctagcgc tcattgccac tgcggtcgtg cttctcgcgg cgctgatcgc agtgcaggcg
     3901 cctcccacgt cactcgacga gctcgcctat cacctggccg tgccctgggc ctgggtcctc
     3961 aaccatcgag ccatcgatct gcctctgatc tcgcactctt acttcccgct cggaatcgag
     4021 tctgccgatc tgccgctgct aacgatcctc ggccagatcc gcgggggaat cgcatcacac
     4081 gttcttcatc tcctggcggc gctggcaacg acggcgatgt tgtttcgcct cgcgcgtggc
     4141 aacgcactcg cggtcgcggc aatcgtcacg acgcccgcgt tggccctcat cgcgggctgg
     4201 tcgctcaacg actggccgct gatcggaatc tgcgccgttc tcgtgctcgc gctcgatcag
     4261 gatgatgtcc cgacgtgggc catcgcactg gccgcgggaa tgctgacgaa gtacacgttt
     4321 gtcccgttcg cggtaatcgc tgtaggtgtt gcgatgtggc g
//
LOCUS       DATLVW010000709         5656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_399926, whole genome shotgun sequence.
ACCESSION   DATLVW010000709 DATLVW010000000
VERSION     DATLVW010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399926"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VNN25_20595"
     CDS             <1..206
                     /locus_tag="VNN25_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH93991.1"
                     /translation="LELPEADRGELAGLLLRSLDPPPDPDVEAAWDAVIDRRMREIDE
                     GTVQTIPWEQVRAEMNARIGKKP"
     gene            complement(234..683)
                     /locus_tag="VNN25_20600"
     CDS             complement(234..683)
                     /locus_tag="VNN25_20600"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH93992.1"
                     /translation="MREIERILDQLNRSWGGPSWTGVDIQPLLDGISEEQARAHPLPN
                     AHSILELTAHMTAWMNAVAARLGGDTRELTPEEDWRDVTQLTWPAAITEMENAQSHLS
                     DVIARLTPDDLDKKVAGRKYTIYVMLHGVIQHNLYHAGQIGLLKKVG"
     gene            734..1522
                     /locus_tag="VNN25_20605"
     CDS             734..1522
                     /locus_tag="VNN25_20605"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXH93993.1"
                     /translation="MSYVVREWLNLAVRWTHVFAAIMWVGQTYFFTWLDHAFHGEKQV
                     WMVHSGGFYIVDKQKRPELLNQTLHWFKWEAFFTLVSGFVLLALVYYDGAIMVDESIL
                     KMSAWQAAGLSLGLIALGWFLYDFLWISPMRNHEVAGAIVSYVLLVGAIVGVTHLFAS
                     RAAYMQIGAMMGSFMALNVWVRILPAQRQLIKAVKEGTEPDMRLADLAKQRSKQNTFI
                     VLPVVFIMISNHFPVATYGNQYNWIVLAVLVLVGWGVAKIIRTR"
     gene            1533..2477
                     /locus_tag="VNN25_20610"
     CDS             1533..2477
                     /locus_tag="VNN25_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93994.1"
                     /translation="MKRTFSDEELRASAAKLDPALTKFMARFPGESGARQPVHTVYGG
                     AHLFKSDTTQKLGVLALRALDEYGPDAATLGNAIGLDSRFRDVVYARVTEKLRREPVE
                     DFRIDFEDGYGNRADAEEDGHAEAAAREVAAGMKNGTLPPFIGIRIKQFSQELMPRSM
                     RTMDIFITTLLDAAGSLPPNFVVTLPKIQLPQQVTALADLFDAMEPKLGLDKHALRFE
                     FMIETTQAILSENGVSNLPLFLDAARPGGGRTLRDVRLHRELQHYRGASADGPSRLRL
                     RTAHDAGRLRRHRPLALRRCHEHHAHRPASRNADAAAG"
     gene            2431..2865
                     /locus_tag="VNN25_20615"
     CDS             2431..2865
                     /locus_tag="VNN25_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93995.1"
                     /translation="MPIAPHRGTLTPQQVEENRNVVHRAWRLHADHIRHSLINAYYQG
                     WDLNPAQLPTRYAAVYSFFLEGLKAATDRLHNFIEKAGQATLVGDVFDDAATGQGLLN
                     YFLRALNCGAITEDEALATGLTLDELRSRSFVKIMKGRREAG"
     gene            3195..3671
                     /locus_tag="VNN25_20620"
     CDS             3195..3671
                     /locus_tag="VNN25_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93996.1"
                     /translation="MEIFHIAVSIDHIEVEILHLDVSIDDNAVEILHNAVSIDDIDVQ
                     IPHIAVSIDHIEVETLHIAVSIDHIDVEILHLDVSIDDNAVEILHLAVSIDDIDVQIP
                     DIAVSIDDIAVEILHNAVSIDHVYVEILDKTQTRWISRPRSAFFVSRVTTLPTFQT"
     gene            3746..5050
                     /locus_tag="VNN25_20625"
     CDS             3746..5050
                     /locus_tag="VNN25_20625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH93997.1"
                     /translation="MSRLRNRFVTRLLIAAAVALFTNTVAMAGVVGSGTPASCTEAAL
                     STQVQAGGTVTFNCGAGPQTIPFTFTMAIGPSNPTVTIDGSDTITLDGTGLTLGMVSV
                     FGNATSLPDVTFKHITFANGNITTGLIAGGAIQNFGKLTLDTVVVRNSHADGNGAIFQ
                     EPCTGCLDPSLTVTRSLFQNNSTGGGGSAITMEGGNASVSDSTFTGNSGPSGGAIYIY
                     SNSTFKVSMSIDRCTFNGNSAVPFVGGAIAVEVLNAGSSVSITNDTFTGNSATGATGQ
                     GSAIYLGASPVTITNCTIAGNSAASGGGAVNFSDRTVVNNTIIASNTGGNCSFASGGT
                     FTGGHNIQYGDSTCFFMTVANPLLGSLADNGGAAKTMALGTGSPAIDAADPAKAPSTD
                     ERGVARTDGNGDGIVVADIGAFEAPTSTTNTNPLGRHHAVRH"
     gene            complement(5105..5383)
                     /locus_tag="VNN25_20630"
     CDS             complement(5105..5383)
                     /locus_tag="VNN25_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH93998.1"
                     /translation="MNINRTVQAGILTLCLAGTPAALAAPQYHRNTEVVDVNAHVLPP
                     GPPHARHVIVVNSAQRAPRHHGVAGMFQRLHQWHMRQRASIYRAVTRR"
     gene            complement(5437..>5656)
                     /locus_tag="VNN25_20635"
     CDS             complement(5437..>5656)
                     /locus_tag="VNN25_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HXH93999.1"
                     /translation="IASGSTDADGRLKFVEEMPEPGLYRISFDTGSYNPEGFFPRVVI
                     EFMVEKGAQHYHVPLLLSPFGYSTYRGS"
BASE COUNT         1099 a         1793 c         1651 g         1113 t
ORIGIN      
        1 cgctggagtt gcccgaggca gatcgaggag agcttgcggg cctgctatta aggagcctcg
       61 atcccccgcc ggatccggat gtcgaggcgg catgggacgc ggtcatagac cgacgcatgc
      121 gagagattga cgagggcacc gtccagacca ttccttggga gcaggtgcgt gcggaaatga
      181 atgcgcggat cggcaagaag ccttaacgtc acccgcgcga cgccatcaac ggctcacccc
      241 actttcttca gcaacccgat ctgtcccgcg tgatagaggt tgtgctggat cacgccgtgg
      301 agcatcacgt agatcgtgta cttgcggccg gcgactttct tgtcgagatc gtcgggggtc
      361 aggcgggcga tcacgtcgga cagatggctt tgtgcgtttt ccatctccgt gatcgcggcg
      421 ggccacgtta gttgggtgac gtcgcgccag tcttcctcgg gggtcagttc gcgggtatcg
      481 ccgccgaggc gggcggcgac ggcgttcatc caggcggtca tgtgggcggt cagttcgagg
      541 atcgagtgcg cgttcggtag tggatgggcg cgggcttgtt cttcgctaat cccgtcgagg
      601 agcggctgga tgtcgacgcc ggtccacgag gggccgcccc aggaacggtt caactggtcg
      661 agaatgcgtt cgatctctcg catggcccgc gattctgcta cattcgcgcc gcaaaccaaa
      721 cataaggatt ccgatgagtt acgtcgttcg tgagtggctg aatctcgcag tccgctggac
      781 ccatgtcttc gccgcgatca tgtgggtcgg gcagacctac ttcttcacct ggctcgatca
      841 cgctttccac ggcgagaaac aggtgtggat ggtccacagc ggcggcttct acatcgtcga
      901 taagcagaag cggccggaac tgctgaatca gacgctgcac tggttcaagt gggaagcatt
      961 cttcacgctg gtgtccggct tcgtactgct cgcgctggtc tactacgacg gcgcgatcat
     1021 ggtcgacgag agcatcctga agatgtccgc ctggcaggct gccggcttga gcctgggcct
     1081 catcgcactc ggctggttcc tctacgactt tctctggatc tcgccgatgc gcaatcacga
     1141 agtggccggc gcgatcgttt cgtatgtgct gctcgtcggc gccatcgtgg gcgtcacgca
     1201 tctcttcgct tcgcgcgccg cgtacatgca gatcggcgcg atgatgggct cgttcatggc
     1261 gctgaacgtc tgggtgcgca tcctcccggc gcaacggcag ctcatcaagg cggtcaaaga
     1321 gggaacggag ccggacatgc gcctggccga tctggccaag cagcgctcca aacaaaacac
     1381 gttcatcgtt ctgccagtcg tcttcatcat gatcagcaat catttccccg tcgccacgta
     1441 cggcaaccaa tacaactgga tcgtgctcgc ggttctcgtc ctggtcggat ggggcgtggc
     1501 gaaaatcatc cgcacgaggt aaagacaatc gaatgaaacg aactttttcc gacgaagagc
     1561 tgcgggcgtc cgcggcgaaa ctcgatcccg cgctcacgaa gttcatggcc cgctttccgg
     1621 gcgaatccgg tgcgcggcag ccggtgcaca ccgtctatgg cggcgcgcat ctgttcaaga
     1681 gcgatacgac gcagaagctt ggcgtactcg ctctccgcgc gctcgacgag tacggtcccg
     1741 acgcagccac cctcggcaac gccatcggtc tcgattccag gtttcgtgat gtcgtctacg
     1801 cgcgtgtgac cgagaaactc cgccgggagc cggtcgagga cttccgcatc gacttcgaag
     1861 acggctacgg caaccgcgct gacgccgagg aagatgggca cgccgaggcg gcggcgaggg
     1921 aagtcgcggc aggaatgaag aacggcacgc tgccgccgtt catcggcatc cgcatcaaac
     1981 agttttcgca ggagctgatg ccgcgctcca tgcgcacgat ggatatcttc atcaccacgc
     2041 tgctcgatgc ggccggttcg ctgccgccaa acttcgtagt gacattgccg aagatccagt
     2101 tgccgcagca ggtgacggcg ctggccgatc tgttcgacgc gatggagccg aagctgggcc
     2161 tggacaagca tgcgcttcgc ttcgagttca tgatcgagac cacgcaggcc atcctcagcg
     2221 agaacggcgt ctccaatctg ccgctctttc tcgatgccgc gcgcccgggc ggtggccgca
     2281 cacttcggga cgtacgacta caccgcgagc tgcaacatta ccgcggagca tcagcggatg
     2341 gaccatcccg cctgcgactt cgcacggcac atgatgcagg tcgcctacgc aggcaccggc
     2401 ctctggctct ccgacggtgc cacgaacatc atgcccatcg ccccgcatcg cggaacgctg
     2461 acgccgcagc aggttgagga gaaccgcaat gtcgttcacc gcgcctggcg cctgcacgcc
     2521 gatcacatcc gccactcgct catcaatgcc tactaccagg gatgggatct caatcccgcc
     2581 cagcttccta cgcgctacgc cgccgtctac tcgttcttcc tcgaggggtt gaaagcggcc
     2641 acggaccggc tccacaactt catcgagaag gcgggacagg ccacgctcgt cggcgatgtc
     2701 ttcgacgacg ccgccacggg ccagggcctg ctgaactact tccttcgcgc cctgaactgc
     2761 ggcgcgatca cggaggatga agcgctggcc accggcctca cgctcgacga gctacgcagc
     2821 cgttcgttcg tgaagatcat gaaggggcgg cgggaagccg gctaggaagt ccctgccaca
     2881 gcgtcagcct ttgttttcgg gccgtcgcag gcatgcatcg aaggccagat cgaccacatc
     2941 gatgtcgaga ttctccacat cgatgtccag atttctaact gcgatgtcca gatgtctaac
     3001 atcgatgtcc agattttaga catcgttgtc gagactttcc acatcgatgt ccagatttct
     3061 aactgccatg tccagatttt ccacatcgaa gtgtcgattt tccacatcga tgtcgaggtt
     3121 tccacatcac agtctcgatc gaccactgcg atgtctagat tttccacatc gatgtctcgc
     3181 ttgaccacat cgaagtggag atttttcaca tcgcagtctc gatcgaccac atcgaagtcg
     3241 agattcttca cctcgatgtc tcgatcgacg acaacgcagt cgagattctt cacaacgcag
     3301 tctcgattga cgatatcgat gtccagattc cgcacatcgc agtctcgatc gaccacatcg
     3361 aagtcgagac tctccacatc gcagtctcga ttgaccacat cgatgtcgag attcttcacc
     3421 tcgatgtctc gatcgacgac aacgcagtcg agattcttca cctcgcagtc tcgattgacg
     3481 acatcgatgt ccagattcca gacatcgcag tctcgatcga cgatatcgca gtcgagattc
     3541 ttcacaacgc agtctcgatc gaccacgtct atgtcgagat tctcgacaaa acccaaactc
     3601 gatggatctc gcggcctcgt tctgctttct tcgtgagccg ggtcaccaca ctcccgacat
     3661 tccagactta acctgtgagg actgcacgcg gttgtccccg ccgagcaccc tggccgaagc
     3721 atcttccgca tcgaaggagc tctacttgag tcgtttgagg aatcgttttg tcacacgtct
     3781 gctcatcgcc gccgcagttg cgttattcac caacaccgtt gccatggccg gtgtggttgg
     3841 aagcggaacg ccagcgtcct gcaccgaagc agccctatcc acgcaagtcc aggccggcgg
     3901 caccgtgacg ttcaactgcg gtgccgggcc gcagaccatc cccttcacgt tcacgatggc
     3961 gatcggacca tcgaacccga cggtcacgat cgacggcagc gacacgatca cattggacgg
     4021 gacgggcctt acgctgggga tggtgagcgt cttcggaaac gccacatcct tgccggatgt
     4081 gaccttcaag cacatcacct tcgctaacgg caacatcaca acggggctga tcgcgggcgg
     4141 agcgattcag aactttggaa aactgacgct cgacactgtc gtggtcagga acagtcatgc
     4201 tgacggaaac ggcgccatct tccaggagcc gtgtaccggt tgcctcgacc cgtcgctcac
     4261 cgtcacgcgc agcctgtttc agaacaacag caccggtggc ggtggctccg cgatcaccat
     4321 ggaaggcgga aacgcttccg tctccgactc gacgttcacc ggcaactcgg gaccgagcgg
     4381 cggagcgatc tacatctaca gcaactcgac gttcaaggtc agcatgtcga tcgaccgctg
     4441 cacgttcaac ggcaactccg cggtgccgtt cgttggcggc gcgatcgcag tggaagtgct
     4501 caacgccggc agcagtgtct cgatcacgaa tgacactttc acaggcaact ccgccacggg
     4561 cgccaccgga caaggttccg ccatttacct cggcgcgtcg ccggtcacga ttaccaactg
     4621 cacgatcgcc ggcaatagcg ccgcttcggg agggggcgcc gttaatttca gcgaccgcac
     4681 cgtcgtcaac aacaccatca tcgccagcaa caccggcggc aattgctcgt ttgcttcggg
     4741 gggcacattc accggaggac acaacatcca gtatggcgat tcgacctgct tcttcatgac
     4801 cgtggccaat ccgctgcttg gctcgctggc ggacaacggc ggcgcggcga agacgatggc
     4861 cctcggcacc ggcagtcccg caatcgacgc tgccgatccg gccaaagcgc cgtcgacgga
     4921 cgagcgcggc gtggcacgaa cggacggcaa cggagatgga atcgtcgtcg ccgatatcgg
     4981 agcgttcgaa gcgcccacct cgactacaaa cacgaacccg ctgggtcgcc atcacgccgt
     5041 tcgccattag acgttcgcat tcacgaaaaa ggccgggcgc gagcccggcc tttggtgtgg
     5101 atccctacct gcgcgtcacc gcgcgataga tgctcgcgcg ctgccgcatg tgccactggt
     5161 gaagccgctg gaacatcccc gcgactccat gatggcgcgg agcgcgttgc gcgctgttca
     5221 cgacgatgac gtgccgcgcg tgcggtggtc ccggcggcag cacatgcgcg ttgacgtcga
     5281 cgacctccgt gtttcgatgg tactgcggtg ccgcgagcgc cgcaggcgtg cccgcgaggc
     5341 acagcgtgag gatgcctgcc tgtactgttc tgttaatgtt catgcttgca cctccagcga
     5401 tggaaagtgc aaggcgctcg cccgttcgcc gcgtcgctaa cttcctcgat acgttgaata
     5461 tccgaacggg ctcaggagca gtggcacgtg gtagtgctgt gcccccttct ccaccatgaa
     5521 ctcgatcacc acccgcggga aaaagccctc gggattgtag gagcccgtgt cgaacgagat
     5581 ccggtacagg ccgggctccg gcatctcctc gacgaacttc agccttccgt ccgcgtctgt
     5641 cgaaccgctg gcgatg
//
LOCUS       DATLVW010000710         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_483180, whole genome shotgun sequence.
ACCESSION   DATLVW010000710 DATLVW010000000
VERSION     DATLVW010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483180"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..2351
                     /gene="ppk1"
                     /locus_tag="VNN25_20640"
     CDS             141..2351
                     /gene="ppk1"
                     /locus_tag="VNN25_20640"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXH94000.1"
                     /translation="MTILADLGISAEGVDAPESVIPIPPSMPLLDRELSALAFNERVL
                     AEARNPAVPLLERVKFLSICAHNLDEFFMIRVGEIRDLIAANVPSEPSGNGLKRLQAV
                     RNGARSLLREIYRCLGEELIPALRKEGIRIERVADLGKKERNFVEEYFGRDLEPILTP
                     LAIDPAHPFPFIANLSLNLVLQLESERGERHVAIIKIPESVPRLVPMPEKTRFVLLED
                     VIASHVHRFFPGLKVLRTAAFRVIRNSDIDIKEEDMQDLLKSIEIELRRRERREVVWL
                     EIEEGVDDPLMALLAGATDSSPDDIFFAPGPLKLGDLQQLHTQIPNAALKEAPFNPRM
                     PAELATTDDIFSIIRRGDLFLHRPYDSFTAVIEFVQTATEDPDVVAIKQTLYRTDPGS
                     PIIAALSAAARAGKQVTAIVELQARFDERKNITWAKTLEEAGVQVVYGIVGYKTHCKV
                     CLIVRREGGELRRYVHLSTGNYNAVTGRLYTDVDLFTCDPAFGADAAQLMNLLTGFSV
                     AGVQELVEAKGGTLRWREFVVSPMDYLRWTIRMIDRETENAKEAKPAFIVAKMNALVD
                     PSVIEALYRASRAGVQIELLVRGICCLVPAMPGFSENIRVTSIIDRFLEHSRIFRFAN
                     GGAPEVWVSSGDWMPRNFLRRIETTFPIRDAAVGKRISEQILAISLADNVKGWTLGAD
                     GTYRRRTPGDQRAVRSQETFISIARADSVSVGPYEESIRHPASSRRKAKKKKKH"
     gene            2363..2749
                     /locus_tag="VNN25_20645"
     CDS             2363..2749
                     /locus_tag="VNN25_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94001.1"
                     /translation="MKPQDLLPKRVVNYIQDIGVRALDHLAAHIDSPPPVATPEGEPA
                     PVNATQTLVDQWKGMSPADKEQFVGRVSGSVMEVIVASATLPPALKKTVKEARKVIRK
                     RVKKVRKAVAKKKKESPKKKSPKKKR"
BASE COUNT          651 a         1079 c          911 g          566 t
ORIGIN      
        1 cagagttcgc ccaggatctg atctctggtc tctgctgtct ctgatctctg cttctcccac
       61 cccccacccc ctacccccca ctccccccgt acaaaattta cacgccctcc cctaatttcg
      121 tgccatacca ttcccgctcc atgactatcc tggctgacct ggggatctcg gctgagggag
      181 tcgacgcccc cgagagcgtc atcccgatcc cgccctcgat gccgctcctc gaccgcgagc
      241 tgagcgcgct cgccttcaac gagcgagtgc ttgcagaagc aagaaatccc gccgtcccgc
      301 tgctggaacg ggtgaagttc ctcagcatct gcgcgcacaa cctcgacgaa ttcttcatga
      361 tccgggtcgg ggagatccgc gacctcatcg ccgcgaatgt cccatccgag ccctcgggca
      421 acggcctgaa acgcctccaa gccgtgcgca acggtgcccg atcgcttctg cgcgagatct
      481 accgctgcct cggcgaagag ctgatccccg ccctccgaaa agaaggcatc cgcatcgagc
      541 gcgtcgccga cctcggcaag aaagagcgca atttcgttga ggagtacttc ggccgcgatc
      601 tcgagccgat ccttaccccg ctggctatcg atccggcgca tccctttccg ttcatcgcca
      661 acctctcgct caaccttgtc ctgcagctcg agtcggagcg gggcgagcgg catgtggcca
      721 tcatcaagat cccggagtcc gtgccgcgcc tggtgccgat gccggagaag acgcgcttcg
      781 tgctgctcga ggatgtgatc gcgtcgcacg tgcatcgctt tttccccggc ctgaaggtgc
      841 tccgcacggc cgccttccgc gtgatccgca attcggacat cgacatcaaa gaggaggaca
      901 tgcaggacct cctcaagagc atcgagatcg agctccggcg ccgggagcgg cgcgaggtgg
      961 tctggctcga gatcgaagag ggagtcgatg atccgctcat ggcgctgctg gccggcgcga
     1021 ccgactcctc acccgacgac atcttcttcg ctcccgggcc gctgaagctc ggcgatctgc
     1081 agcagctcca cacgcagatt ccgaatgcgg cattgaagga agcgcccttc aatccgcgca
     1141 tgccggccga gctggcgacg acggacgaca tcttttcgat catccggcga ggggatttgt
     1201 ttctccaccg gccatacgac tcgttcacag ccgtgatcga gttcgtgcag acggcgactg
     1261 aagatcccga tgtcgtcgcc atcaagcaga cgctttaccg taccgatccc gggtcaccga
     1321 tcatcgcagc cctttccgcc gcggcgcggg cgggaaagca ggtcacggcg attgtcgaac
     1381 tgcaggcgcg cttcgacgaa cggaagaaca tcacctgggc gaagacgctc gaagaagcag
     1441 gcgtgcaggt ggtctacgga atcgtcggct acaagacgca ctgcaaagtc tgcctcatcg
     1501 tccggcgtga agggggcgag cttcgccgct acgttcatct gtcgaccggc aattacaacg
     1561 ccgtcaccgg ccggctctac acagacgtcg atctcttcac ctgcgatcct gccttcggcg
     1621 ccgacgccgc gcaactgatg aatctcctca ccggtttcag cgtggcgggc gtgcaggaac
     1681 tggtcgaagc gaagggcggg acgctgcgct ggcgcgagtt cgtcgtctcg ccgatggact
     1741 acctgcgctg gacgatccgc atgatcgacc gcgagacgga gaacgcgaag gaagcgaagc
     1801 ctgccttcat cgtggccaag atgaacgcgc tggtcgatcc gagcgtcatc gaagcgctgt
     1861 accgcgccag ccgcgccggc gttcagatcg agctgctcgt gcgcggtatc tgttgcctcg
     1921 tgcccgcgat gcccggattc agcgagaaca tccgcgtcac ctcgatcatc gatcgcttcc
     1981 tcgagcactc gcgcatcttc cgcttcgcca atggcggcgc gccggaggtg tgggtgtcga
     2041 gcggcgactg gatgccgcgc aacttcctgc gccgcatcga gacgacgttc ccgatccgcg
     2101 atgccgccgt ggggaagcgg atcagcgaac agatcctggc gatctcactg gccgacaacg
     2161 tcaaaggctg gaccctcggc gccgacggca cctaccgccg ccgaacaccc ggcgaccagc
     2221 gcgctgtccg cagccaggaa acattcatct cgatcgcacg cgccgattcg gtgagcgtcg
     2281 ggccgtacga ggagagcatc cgacatccag cctccagccg ccgcaaagcg aagaagaaga
     2341 aaaagcatta ggaaggaagc gcatgaaacc gcaggatctc ttgccgaaac gcgtggtcaa
     2401 ctacatccag gacatcggcg tccgcgccct cgatcacctc gccgcccaca tcgattcccc
     2461 tcctccggtg gccacgccgg agggcgagcc tgctcccgtc aacgccacgc aaacgctcgt
     2521 cgatcaatgg aaggggatgt ctcccgccga caaagagcag ttcgtcggcc gagtctccgg
     2581 ctccgtgatg gaagtgatcg ttgcctccgc cacgcttcca cccgcgttga agaagaccgt
     2641 gaaagaggcc aggaaagtca tccgcaaacg cgtgaagaaa gtgcgcaaag cggtcgcgaa
     2701 aaagaagaaa gagtcgccga agaagaagtc gccaaagaaa aagaggtagc aaggagtgca
     2761 ggcgtctcgc ctgcggccga cgggcgtccc gcccggcggt gaatccgcga cgatgcgcag
     2821 aagggtcctg catcacgaaa ctcacgccga tgccgcccca ggcgagacag gatctccgct
     2881 gcgcgagcgg ggcacggctg cgtcccaaac actcgctccg cgaaagtgac atcttttttc
     2941 gccctgtcgt ggaacctgtt caaaactctt ccaccaattt cgcaaacagc agcgcaaaga
     3001 tcgtcgagat tcttgccaac aacccccaga ttctccgggt caacctctga gctctcagac
     3061 atcaacctcc agattctcga tgtcaacctc tggactctca gagatcaacc tccagattct
     3121 cgaggtcaac ctctagattc tagagatcaa cttccagatt ctcgatgtca acgtccagat
     3181 tctcgatgtc aacctctgga ttctcag
//
LOCUS       DATLVW010000711         5157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_566018, whole genome shotgun sequence.
ACCESSION   DATLVW010000711 DATLVW010000000
VERSION     DATLVW010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566018"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..777)
                     /locus_tag="VNN25_20650"
     CDS             complement(73..777)
                     /locus_tag="VNN25_20650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH94002.1"
                     /translation="MTKILVVEDEAGLRQGLEINLRMENYETALAADGEEAVRMFHAE
                     SPDLVLLDLMLPKKSGFEVLDHIRKFSKIPVILLTARGQETDKVRGLRGGADDYITKP
                     FGLQELFARIDAVMRRSDATSVTTKTVCEDTLIKLDPSTREASAKDQKLKLSPKEFDL
                     LMYLFRNKDRVVPRDELLSRVWNYRVDLNLNSRTVDTHVTRLRRKLQKAVGTDAAVIV
                     AVAKIGYRYLGEATAA"
     gene            complement(798..2363)
                     /locus_tag="VNN25_20655"
     CDS             complement(798..2363)
                     /locus_tag="VNN25_20655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXH94003.1"
                     /translation="MLRALMAKWKVSARAGDGPNHVSAILIPFLIVTAGLAVLAWRSY
                     VLSERMERGVNTLAMQYAGYAADISARRIDGAVGAELFRASEEWQGVERRSPSAIELK
                     KWIHDNDWIISALYVPDADPASAIFAISQSGGTHPTPLTREFYSSSGTVRYTYDPDLL
                     IARVYPTLANQPLVQTAGLSQEAQVGVIKYPSATGAIKSEHGYAFIAPLAPPLSSYAI
                     RGFVETGYGTSGWENARLISLLLSAIAMLLTALGLYLAMRGLTREADTMKLRGALVAN
                     VSHELRTPLSMIRLGAETLKRGANRLPEKQRHEIEDSILREVLHLSHLVENVLDIARI
                     QNNSARALAFTPVYPRDLLTSLISTYESWVRSRGFEITLHIDDSVEEQSWDRDAVSRA
                     VLNLIDNATKYAGDTNKRIDVVLRQNTQSIIIEVRDHGLGIDADDLTHIFEPYFRAHF
                     SDTQTRRGAGLGLTLVQQIVASHGGSIEVESKPGLGSTFRLLFPRPQSSQTASPGAAR
                     NTNALETLAVQRS"
     gene            complement(2373..2843)
                     /locus_tag="VNN25_20660"
     CDS             complement(2373..2843)
                     /locus_tag="VNN25_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXH94004.1"
                     /translation="MNVRLSHFLIAALLVAPAAFADDTKLCNASARECEQAIRQLSSG
                     RRYLGASIQELNPGIRVKAVIEDGPAERADLRPGDRLMAINGHGTRSASIKDFKQLLY
                     DAKETGVLWVIVQRDGILKKIEVRLEPYTKAQIDKMVAQHLAQFHAIATTAPPQ"
     gene            complement(2964..3584)
                     /locus_tag="VNN25_20665"
     CDS             complement(2964..3584)
                     /locus_tag="VNN25_20665"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HXH94005.1"
                     /translation="MTVSTLAVPRADFSIEEPWLVPKECVPVMLRRSTDGASPRQATS
                     VAAYYDDEFLTVVFQADDDEIVATFLGHDEPLWQEDVFEVFLAPDGLTPYFEIEVNPL
                     GTTFDARIDSPDGVQATMITDLAWTCEPLFAGLRRDDNRELRAVIRIPFRAMGRTPKP
                     GEQWRGNFFRIDRSRAHGDDFSAWQPAMKTPPDFHVPAAFGTLLFT"
     gene            3657..3977
                     /locus_tag="VNN25_20670"
     CDS             3657..3977
                     /locus_tag="VNN25_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXH94006.1"
                     /translation="MVDLELDELKREFLAEADEKVREIQEKLDAEQSADVLERLTYLA
                     HQLKGSGGSYGYQRISTDAAELEKAVELRAKDGAQPGIDDKIQQHVFNLRAEVDRRVK
                     ELAV"
     gene            complement(4129..>5157)
                     /locus_tag="VNN25_20675"
     CDS             complement(4129..>5157)
                     /locus_tag="VNN25_20675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH94007.1"
                     /translation="TWQGPRNMALRLRQTAPMPDSQPFQVAVVDDDPAIRRLVRLLLK
                     RGGYQSVEFSTGEEARAQLPALQWDLAILDRRLPDMDGVVLCQQLKADPALRNRYIIM
                     LTGEDDQEDKIQGLDLGADDYITKPFQYPELMARIRAGKRIVDLQKELVESNRRLEQL
                     SITDGLTKLHNHRHFQDELLRAFEESARYERPLSLAIVDLDFFKKVNDTYGHATGDEV
                     LKTISKIFQQSIRTTDLAARYGGEEFALMLPETSLSDAMNFAEKIRELVEDMRIETQA
                     GVISATVSVGVATVPHPKIHSPKELIVAADKALYRAKKNGRNQVQAERRSDPDRARAV
                     KSGEGTAA"
BASE COUNT          951 a         1508 c         1689 g         1009 t
ORIGIN      
        1 gtatggcggc ggcaacaagt ggtcgtgttc atgtgagcgg tgacgctatg actgtttggc
       61 tttggttgtc aatcacgctg ccgtcgcctc gcccagatac cgatacccga tcttggcgac
      121 ggcgacgatc acggcggcat cggtaccgac cgccttctgc agtttgcggc ggaggcgcgt
      181 tacgtgggtg tcgaccgtac ggctgttgag attgaggtcg acgcgatagt tccagacgcg
      241 cgagaggagc tcgtcgcgcg gcacgacgcg atccttgttg cggaagagat acatgagcag
      301 atcgaactct ttcggggaga gcttgagctt ctgatccttg gccgacgctt cgcgcgtgga
      361 cggatcgagc ttgatgagcg tgtcttcgca gacggtcttg gtagtgacgg acgtggcgtc
      421 gctgcggcgc atgaccgcat cgatgcgcgc gaaaagctcc tgaaggccga acggcttggt
      481 gatgtagtca tcagcgcccc cgcgcaggcc gcgcaccttg tccgtctcct gtccgcgggc
      541 ggtcaggaga atgaccggaa tcttggaaaa cttgcggatg tggtcgagga cttcgaagcc
      601 gctcttcttc ggcagcatca ggtcgagcaa cacgagatcg ggcgattccg cgtgaaacat
      661 gcgcaccgcc tcctcgccat cggccgccag cgccgtctcg tagttctcca tacggagatt
      721 gatttcgagt ccctgccgga gtccggcttc atcctcaacg accagaatct ttgtcattcg
      781 cgagtctccg ctggtgctta cgaacgttgt acggcaagag tttccagagc gttagtgttg
      841 cgagctgcgc caggggatgc tgtctgcgac gattgaggcc gcgggaaaag aaggcggaat
      901 gtcgagccga ggccgggttt cgactccact tcgatgctgc cgccgtgtga cgccacgatc
      961 tgctgcacca gtgtcagtcc cagcccggca ccgcgtctcg tctgcgtatc ggagaagtgg
     1021 gcgcggaagt acggctcgaa gatatgcgtc aggtcgtcgg catcgatccc gagaccgtga
     1081 tcgcgcactt cgatgatgat cgactgggtg ttctggcgca acacgacgtc gatccgcttg
     1141 ttcgtgtcgc cggcgtactt cgttgcgttg tcgatgaggt tcaggacggc gcgtgagacg
     1201 gcatcgcggt cccacgactg ctcctcgacg gagtcatcga tgtggagcgt gatctcgaag
     1261 ccgcggctgc ggacccagga ttcgtaggtc gaaatgagcg acgtaagcag gtcgcgcgga
     1321 tagaccggtg tgaaggccag ggccctggcg ctgttgttct ggatgcgggc gatgtcgagc
     1381 acgttctcga cgaggtgcga gaggtggagc acctcgcgca gaatcgaatc ctcgatttcg
     1441 tggcgctgct tctccggaag acggttcgcg ccgcgcttga gcgtctccgc gccgaggcgg
     1501 atcatcgaaa gcggcgtgcg caattcgtgc gagacgttgg ccacgagcgc gccgcgcagt
     1561 ttcatcgtgt ctgcctcgcg cgtcagaccg cgcatcgcca ggtagaggcc gagggcagtg
     1621 agcagcatgg cgatggcgct gagcaggagg ctgatcagcc gggcgttctc ccaacccgag
     1681 gtgccgtagc cggtctcgac gaacccacga atggcgtacg acgacagagg tggcgccagc
     1741 ggcgcgataa aggcataacc gtgctcgctc ttgattgcac cggtcgccga gggatacttg
     1801 atcaccccaa cctgtgcttc ctgggacagc ccggcggttt gcacgagggg ctgattggcg
     1861 agcgtgggat agacgcgggc gataaggaga tcgggatcgt aggtgtagcg cacggtgccg
     1921 ctggatgagt aaaactcccg cgtcagcggc gtggggtgcg tgccccccga ctgcgagatg
     1981 gcgaagatgg cgctggccgg atcggcgtcc ggaacgtaga gcgcggagat gatccagtcg
     2041 ttgtcgtgaa tccacttttt cagttcgatg gccgaagggg agcgccgctc cactccctgc
     2101 cactcttccg aggcgcggaa caactcagcg ccgacggcgc catcgatgcg gcgggcgctg
     2161 atgtcggcgg cataaccggc gtactgcatc gccagtgtgt tgacgccgcg ctccatccgt
     2221 tcggagagca cgtacgagcg ccacgccagg acggccaggc cggcggtgac gatgaggaac
     2281 ggaatcagga tggctgagac gtgattcggt ccgtcacccg cgcgggcgct gaccttccac
     2341 ttcgccatca aggcacgcag catgtctcac cattattgcg gcggcgccgt cgtggcgatg
     2401 gcatggaact gagccagatg ctgcgccacc atcttgtcga tctgcgcttt cgtgtacggc
     2461 tccaaccgca cttcgatctt tttcaggatg ccgtcgcgct gcacgatgac ccagagcacg
     2521 ccggtctcct tcgcgtcgta aagaagctgt ttgaaatcct tgatgctcgc cgagcgcgtg
     2581 ccgtggccgt tgatggccat cagccggtcg ccggggcgaa ggtcggcgcg ttccgcgggg
     2641 ccgtcctcga tgacggcctt gacccggatt ccgggattga gctcctgaat tgacgcgccg
     2701 aggtagcggc gcccgctgga aagctgccgg atggcctgct cgcactcgcg cgcggaggca
     2761 ttgcatagct tggtgtcgtc ggcgaacgcc gccggagcga caagaagagc cgcaatcaga
     2821 aaatgagaga gccgaacatt catgagttgg gaacggggtc cttggccggt catcaatcta
     2881 atgcgttacc ggggggacgt tgattacaaa tcagaaaatg tcgaatgaat tccctgtaat
     2941 acaacaggtt agctgcattt cgatcatgtg aacaaaaggg tgccgaacgc tgccggaacg
     3001 tgaaagtcgg gaggcgtttt catcgccggc tgccaggcgc tgaagtcgtc tccgtgcgcg
     3061 cggctgcggt cgatccggaa gaagtttccg cgccactgct cacccggctt cggcgtgcgt
     3121 cccatcgctc ggaaggggat acggatgacg gcccgcagct cccggttgtc gtcgcgccgc
     3181 agcccggcga atagtggctc gcaggtccag gcgaggtcgg taatcatcgt tgcctgcact
     3241 ccgtccggcg agtcgatccg cgcgtcgaag gtggtcccga gcggattgac ctcgatctcg
     3301 aagtagggag tgagcccatc cggcgcaagg aagacttcga agacatcttc ctgccacaac
     3361 ggctcgtcgt gtcccaggaa cgtcgccacg atctcgtcgt cgtcggcctg gaacacgacg
     3421 gtcaggaact catcgtcgta gtaggctgcg accgaggtgg cttgccgcgg agaggcgccg
     3481 tccgtgcttc gccgcagcat gaccggtacg cactcttttg gaacgagcca cggctcttcg
     3541 atcgagaaat cggcacgcgg cacggcgagg gttgagaccg tcattcatgg aaatcatagc
     3601 ggtcccggcg atgccgcggc atatcacgtg caggagcggg gcggggcccg acgatgatgg
     3661 ttgatctgga acttgatgag ctgaagcgcg agtttctcgc cgaagcggac gaaaaagttc
     3721 gcgaaatcca ggagaaactg gatgccgagc agtccgccga cgtgctcgag cgcctgacgt
     3781 atctcgccca ccaactgaaa ggctccggcg gcagctacgg ctaccagagg atctcgacgg
     3841 atgccgcgga gctggagaag gccgtcgagc ttcgcgcgaa agacggcgcg cagcccggaa
     3901 tcgacgacaa gatccagcag cacgtcttca acctgcgtgc agaggtcgac cggagggtga
     3961 aggagctggc ggtgtgacag cagagatcag aaagcagaaa agcagagatc agagatcagt
     4021 ggattagcga tcggtggatc agtagatcag atctgattcc gtctactaat ctcgaatctc
     4081 tgatctcgaa tctctgatct ctgatctctg ttcccctgat ctctgctctt acgccgccgt
     4141 cccctcccca ctcttcaccg cacgcgctcg atcaggatcg ctgcgccgtt ccgcctgcac
     4201 ctgattccgg ccgttcttct tggctcgata gagagccttg tcggcggcca cgatcagctc
     4261 cttcggtgag tgaattttcg gatgcggcac ggtggccacg cccacgctga cggtagcgga
     4321 gatcactcct gcctgcgtct cgatccgcat atcttcgacg agctcccgga ttttttcagc
     4381 gaagttcatg gcgtcgctca gcgacgtctc cggcagcatc agggcgaact cctcgccgcc
     4441 gtagcgtgcc gccagatcgg tggtccggat cgactgctgg aagatcttcg aaatggtttt
     4501 gaggacttca tcgccggtgg catggccgta ggtatcgttg accttcttga aaaagtcgag
     4561 atcgacgatg gccagtgaca gcggccgctc gtagcgcgcc gactcctcga aagcgcgcag
     4621 caactcatcc tgaaagtggc ggtgattgtg aagcttcgtc aggccgtcgg tgatggagag
     4681 ctgttccaga cgccggttgc tctccacgag ctccttctgc agatcgacga tgcgcttgcc
     4741 ggcccggatg cgcgccatga gctcggggta ctggaacggc ttcgtgatgt agtcgtcggc
     4801 gccgaggtcg aggccttgga tcttgtcctc ctgatcgtcc tcgccggtga gcataatgat
     4861 gtaacgattg cgcagcgccg gatcagcttt gagctgctgg cagagcacga cgccgtccat
     4921 gtccggcaga cgcctgtcga gaatggccag atcccactgc agcgccggca gttgggcccg
     4981 cgcttcttct ccggtgctga attcgacgct ctggtaaccg ccacgcttga gcagaaggcg
     5041 caccagtcgc cggatggccg gatcgtcatc gacgaccgcg acttgaaagg gctgggaatc
     5101 gggcatcggg gccgtctgcc gcaaacggag tgccatattg cgcggcccct gccaagt
//
LOCUS       DATLVW010000712         5220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_440905, whole genome shotgun sequence.
ACCESSION   DATLVW010000712 DATLVW010000000
VERSION     DATLVW010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440905"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VNN25_20680"
     CDS             <1..1702
                     /locus_tag="VNN25_20680"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXH94008.1"
                     /translation="PETHYLESWSDARAHDGTISIIQPLIAPLYNGRSQHELLGALLG
                     GMDQSPYDVVHNYWQTKAGALNFNNTWRRWLNDGVIAGSALPARAAGATPALPGAPTP
                     DTRPPAAYELRILPDPTIYDGRFANNGWLQELPKPQTKITWENVALISPKTAVAIGYD
                     DKRRDELVNEKQTLIANIRYRGKTLRTPIWVVPGHADNTITMFLGYGRTRGGKLATPA
                     EASSMLAPELAAFHLSGGTAGVDVAPFRFADAPNGGSGAEIELTSDAPYEIACTQEHQ
                     SLNVSDVGEDRGIIRSATFAEYLAKPDFVRDHKREEEQSKESMYPGFEYKSHAWAMVI
                     DTSVCTGCNGCVVACQSENNIPIVGKDQVVAEREMHWLRIDRYYKGDAANPEVFHQPV
                     PCMQCESAPCEPVCPVGATSHSGEGLNDMTYNRCVGTRYCSNNCPYKVRRFNFFHYSD
                     YETPALKAQRNPDVTVRTRGVMEKCTYCVQRINAAKINAEKDGKSGRRVRDGEVVTAC
                     QQACPTNAIVFGDMNDPSSQVAALKSEPTNYALLAELNTRPRTTYLGAIRNPNPEIKG
                     "
     gene            1802..3220
                     /gene="nrfD"
                     /locus_tag="VNN25_20685"
     CDS             1802..3220
                     /gene="nrfD"
                     /locus_tag="VNN25_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXH94009.1"
                     /translation="MPDQTPAHEIRPVTQEIPERFIPVPTVEPGHTFASVTDHISAIV
                     LRKKMPFLWWVGLALSCFGVMVLTATIGKLVLVGVGIWGINHPIGWGFDILNFVWWIG
                     IGHAGTLISAILLLLRQEWRTSINRFAEAMTLFAVSAAGLYPALHTGRPWYDFWLFPY
                     PNTMTLWPQFRSPLMWDVFAVSTYATVSALFWYVGLIPDLATLRDRARGNVARVIYGM
                     LSLGWRGSAKHWFRYESTYLLLAGLSTPLVLSVHTIVSFDFAVAQLPGWHATFFPPYF
                     VAGAVYAGFAMVLTLAIPARKMFHLEDFITMKHLENMGKIMLATGLIVFYSYCMEAFF
                     AYYSGNNFERYMWINRVSGPYWKMYAMLIICNGVAPQMMWFRRMRTSTMGLFVVAMFV
                     NVGMWLERFVIIPTSLHRDFLPSSWGMYHGTRWDWGMFIGSMGLFFFLLFLFVRFLPM
                     ISIFEMRTMLPEAQAHPEGHEA"
     gene            3224..3781
                     /locus_tag="VNN25_20690"
     CDS             3224..3781
                     /locus_tag="VNN25_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HXH94010.1"
                     /translation="MAIGKVRTGTYGIIAEFSSPADLTAAAEAARDAGYTQMDAFSPF
                     PIHNLHEAVGMKSTGLSSMSAIVLIGGIIGGIAGFFMCWYANVISYPLNIGGKPFNSW
                     PAWIPITFECTILMAAFAAVFGMLALNGLPMPYHPVFNVKRFDQASRDKFFLVIQARD
                     PKFDLDTVWKFLEALHPREVIDVPW"
     gene            3768..4406
                     /locus_tag="VNN25_20695"
     CDS             3768..4406
                     /locus_tag="VNN25_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXH94011.1"
                     /translation="MFPGSAITQRNAGVSPAGPAASRRRGVALIVLLIALTACRQKMS
                     NQPKYDPLEPSDFFADGMSARPRIPGTVARGELANDTFLATGKINGADGDGYPFPITA
                     AVMDRGQERFNIYCSVCHGRAGDGNGMIPSRGLRHPPSYHTDALRNAKTGHFFDVMTN
                     GFGAMPSYAVQIPVADRWAIIAYVRALQLSQNGTINDLPADQRAKLTQGATQ"
     gene            4415..>5220
                     /locus_tag="VNN25_20700"
     CDS             4415..>5220
                     /locus_tag="VNN25_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94012.1"
                     /translation="MNETTASATGISQWRTPALIIGIIGLALSALGYFINQETFFRSY
                     LPSFLFWFSIVAGSLAVLMLQYVTGGEWGLMIRRPLGAAARTMLWMVLFFIPILLGMK
                     YLYPWSNTQWAMADENVSQKLDWLNWKRFLAFAALYFALWNLWAWRIRALSLSFYEDR
                     SPYTELKRRKWAASGLLMIVLTLTFASIDWMMSTEPKWSSTMYGITFVVGCALSALAF
                     VTFFLTRLVNTEAMGSLLKPTHLRDLGNLMLAFVMFYAYTTFSEFLLIWYA"
BASE COUNT          938 a         1784 c         1504 g          994 t
ORIGIN      
        1 ccccgagacg cattacctcg agtcatggag cgacgcccgc gcgcacgacg ggacgatctc
       61 gatcatccag ccactcatcg cgccgctcta caacggccga tcgcagcacg agcttctcgg
      121 cgcactcctc ggcggcatgg atcagtcgcc ctacgatgtc gtgcacaact actggcagac
      181 gaaagccggt gcgctcaact tcaacaacac ctggcggagg tggctgaatg acggcgtgat
      241 cgccggctca gcgctgcccg cgcgcgcggc cggcgcgacg cccgcgctcc caggcgctcc
      301 gacacccgac acccgccccc ccgcggccta cgaattacgc atcctcccgg atcccaccat
      361 ctacgacggc cgtttcgcca acaacggatg gctccaggag ttgccgaaac cgcagacgaa
      421 gatcacgtgg gagaacgtcg cgctgatcag ccccaagact gcggtggcca tcggttacga
      481 cgacaaacgc cgcgacgagc tggtcaacga aaagcagacg ctgatcgcga atatccgtta
      541 tcgcggcaag acgctgcgca cgccgatctg ggtcgtgccc ggccatgccg acaacacgat
      601 caccatgttt ctcggttacg gccggacgcg cggcggcaag ctggccactc ctgctgaagc
      661 cagcagcatg ctcgctccgg aactcgccgc gttccacctt tcgggcggca ccgcgggcgt
      721 cgatgtcgct cccttccgct tcgcggatgc gccgaacggc ggcagcggag cggagatcga
      781 gctgacgtcg gacgcgccat acgagatcgc ctgcacgcag gagcaccaga gcctcaacgt
      841 cagcgacgtc ggcgaagacc gcggcatcat ccgctccgcg acgttcgccg agtacctcgc
      901 caagcccgat ttcgttcgcg atcacaagcg cgaagaagag cagagcaagg agtcgatgta
      961 cccgggcttc gaatacaagt cgcatgcatg ggcgatggtg atcgacacct cggtctgcac
     1021 cggctgcaac ggttgcgtcg tggcctgcca gtccgagaac aacatcccca tcgtcggcaa
     1081 ggatcaggtc gttgccgagc gcgagatgca ctggctgcgc atcgaccgct actacaaggg
     1141 cgacgccgcg aatccagaag tgttccatca gcccgtgccg tgcatgcagt gcgagagcgc
     1201 cccctgcgag ccggtctgcc cggtcggcgc gacgtcccac agcggcgaag gtctgaacga
     1261 catgacctac aaccgttgcg tcggcacgcg ctactgctcg aacaactgcc cgtataaagt
     1321 gcggcgcttc aacttcttcc actactccga ttacgaaacg ccggcgctca aagcgcagcg
     1381 caatcccgac gtcaccgtcc gcacgcgcgg cgtcatggag aagtgtacgt actgcgtgca
     1441 gcgcatcaac gccgcgaaga tcaatgccga gaaggatggc aagagcggac gccgcgtccg
     1501 cgacggcgag gtcgtcaccg cctgccagca ggcctgcccg accaacgcca tcgtcttcgg
     1561 cgacatgaac gatccatcga gccaggtcgc tgcgctcaag tccgagccga cgaactacgc
     1621 gctactcgcg gaactgaaca cgcggccgcg cacgacatac ctcggcgcga tccgtaaccc
     1681 gaatccggag atcaagggat aaccaaatgt tgaaagtctt gaaccacaaa gacacaaagg
     1741 acacaaagaa aaccctttgt gctctttgtg cctttgtggt gaaacgcctt tcttgacagt
     1801 catgccagac caaacaccag cacacgaaat acgcccggta acgcaggaga tcccggagcg
     1861 tttcatcccc gtgccgacgg tcgagccggg gcataccttc gcgtcggtca cggatcacat
     1921 cagcgccatc gttctgcgca agaagatgcc tttcctctgg tgggtcggac tggcgctgtc
     1981 ctgcttcggc gtgatggtgc tgaccgcgac gatcggcaag ctcgttctcg tcggcgtcgg
     2041 catctggggc atcaaccatc ccatcggctg gggcttcgac atcctcaact tcgtctggtg
     2101 gatcgggatc gggcacgccg gcacgctgat ctccgcgatt ctgctgctgt tgcgtcagga
     2161 gtggcgcaca tcgatcaacc gattcgcgga agcgatgacg ctcttcgcgg tctcggcggc
     2221 cggcctctat ccggcactgc acaccggacg gccgtggtac gacttctggc tcttccctta
     2281 cccgaacacg atgacgttgt ggccgcagtt ccgcagcccg cttatgtggg acgtcttcgc
     2341 agtctcgacc tacgccacgg tctcggcgct cttctggtac gtcggtctga tccccgactt
     2401 ggccacgttg cgcgaccgcg cccgcggcaa cgtcgctcgc gtcatctacg gcatgctctc
     2461 cctcggctgg cgcggttccg cgaagcactg gttccgctac gagtcgacgt acctgctgct
     2521 ggccggtctc tccacgccgc tcgtgctctc cgtccacacg atcgtctcgt tcgacttcgc
     2581 tgtcgcgcag ttgccgggat ggcacgccac gttcttcccg ccgtacttcg tcgcaggcgc
     2641 cgtctacgcc ggtttcgcca tggttctcac tctggccatt ccggcgcgca agatgttcca
     2701 cctcgaggac ttcatcacca tgaagcacct cgagaacatg ggcaagatca tgctggccac
     2761 gggtctgatc gtcttctact cgtactgcat ggaggcgttc ttcgcgtact acagcggcaa
     2821 caacttcgag cgctacatgt ggatcaaccg cgtgtccggg ccgtactgga agatgtacgc
     2881 gatgctgatc atctgcaacg gtgtcgctcc gcagatgatg tggttccgca ggatgaggac
     2941 gtcgacgatg ggcctcttcg tcgtggcgat gttcgtcaac gtcggcatgt ggcttgagcg
     3001 cttcgtcatc atcccgacgt cgctgcaccg cgacttcctg ccgtcctcgt ggggcatgta
     3061 tcacggcacc cgctgggact gggggatgtt catcggatcg atggggctct tcttcttcct
     3121 cttgttcctc tttgttcgat tccttccgat gatctcgatt ttcgagatgc ggaccatgct
     3181 gcccgaggcg caggcgcatc cggaagggca cgaggcttaa gcgatggcga tcggcaaagt
     3241 cagaaccggg acgtacggca tcatcgcgga gttctcttcg cctgccgacc tcaccgctgc
     3301 cgccgaagcc gcgcgcgacg ccggctacac gcagatggac gccttctcgc cgtttccgat
     3361 ccacaacctg catgaagcgg tggggatgaa atcgaccggt ctctccagca tgtcggccat
     3421 cgtcctgatc ggcgggatca tcggcggcat cgccggattt ttcatgtgct ggtacgccaa
     3481 cgtcatcagc tatccgctca acatcggcgg gaagccgttc aactcgtggc cggcctggat
     3541 tccgatcacc ttcgagtgca cgattctcat ggctgctttc gcagccgtgt tcggcatgct
     3601 cgccctcaac ggcctgccga tgccgtatca ccccgtgttc aacgtgaagc gcttcgatca
     3661 ggcgtcgcgc gacaagttct tcctcgtcat ccaggcccgc gatccgaagt tcgatctcga
     3721 caccgtctgg aagtttctcg aggcactcca tccccgcgag gtcatcgatg ttccctggta
     3781 gcgcaattac gcagcggaac gccggcgtct cgccggctgg cccggcggcc tcccgccgcc
     3841 gcggcgttgc gctgatcgtg ttgctcatcg ccctgaccgc ctgccgccag aaaatgtcca
     3901 accagcccaa atacgatccg ctcgagccga gcgatttctt cgccgacggt atgtccgcac
     3961 gcccgcgcat ccccggcaca gtcgcccgcg gtgagctggc caacgacacc ttcctcgcca
     4021 ccggcaaaat caacggcgca gacggcgacg gttacccctt cccgatcacc gcggcagtca
     4081 tggatcgcgg gcaggagcgc ttcaacatct actgctccgt ctgtcacggc cgcgccggcg
     4141 acggcaacgg catgattccc tcgcgcggcc tgcgccatcc gccgtcgtac cacaccgacg
     4201 cgttgcggaa cgcgaagacg ggacacttct tcgacgtgat gaccaatgga ttcggggcca
     4261 tgccttcgta cgcggtgcag attccggtcg ccgatcgatg ggccatcatc gcctacgtcc
     4321 gcgcgctgca attgagccag aacgggacga tcaacgacct gcccgcagat cagcgtgcga
     4381 agcttacgca gggggcaacg cagtgagcgc gggcgtgaac gaaaccaccg cctctgccac
     4441 cggcatcagc cagtggcgca cgccagcgct gatcatcggg atcatcggtc tggctctcag
     4501 cgcgctcgga tacttcatca accaggaaac gttcttccgc tcgtacctcc ctagcttcct
     4561 cttctggttc tcgatcgtcg ccggctcgct cgccgtgctg atgttgcaat acgtcaccgg
     4621 cggcgaatgg ggcctcatga tccgccgtcc gctcggcgcg gcggcccgca cgatgctctg
     4681 gatggtgctc ttcttcatcc ccatcctcct cggcatgaag tacctctatc cgtggtccaa
     4741 cacgcagtgg gcgatggccg acgagaacgt cagccagaaa ctcgactggc tgaactggaa
     4801 gcgcttcctg gcctttgccg cgctctactt cgcgctctgg aacctctggg cgtggcgcat
     4861 ccgtgcgctg tcgctcagct tctacgaaga ccgctcgccg tacacggagc tcaagcggcg
     4921 caagtgggct gcctccggac tgctgatgat cgtcctgacg ctgacctttg cgtcgatcga
     4981 ctggatgatg tcgaccgagc cgaaatggag ctcgacgatg tacggcatca cgttcgtcgt
     5041 cggttgcgcg ctctcggctc tggccttcgt cacgttcttc ctcacccgcc tggtgaatac
     5101 cgaagcgatg ggctccctgc tcaagccgac gcacctccgc gacctcggca acctgatgct
     5161 ggcgttcgtc atgttctacg cctacaccac cttctccgag ttcctcctca tctggtacgc
//
LOCUS       DATLVW010000713        11285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_400018, whole genome shotgun sequence.
ACCESSION   DATLVW010000713 DATLVW010000000
VERSION     DATLVW010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400018"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VNN25_20705"
     CDS             <1..531
                     /locus_tag="VNN25_20705"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXH94013.1"
                     /translation="VPVLDMLLNAVKESPGDRVKFVAGGATRLQSVAAGLAAAGDEFD
                     LVAIHDAVRPFFSPDTFHATLDAARTHGAAFPGIPLPDTIHEIKDDSIASTPDRSVFV
                     AAQTPQCFRIDVLRDILTRAAREDANATDEAGLAAKYGHRVQLVPGDSMNFKITRPED
                     LVMAERIYAEWGGGEE"
     gene            932..1408
                     /gene="ispF"
                     /locus_tag="VNN25_20710"
     CDS             932..1408
                     /gene="ispF"
                     /locus_tag="VNN25_20710"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXH94014.1"
                     /translation="MIRVGQGIDVHQFADARPLILGGVRISDTGGLSGHSDADAVLHA
                     VTDALLGAAGAGDIGQYFPSEDPRWKDADSTIFLREAKRIIDEMDAEIGNVDVTIIAQ
                     TPKLAPFRDAIQASIASLLNLPAGHVNVKATTTDHLGFIGRSEGICAMAIVSISIS"
     gene            1421..1822
                     /locus_tag="VNN25_20715"
     CDS             1421..1822
                     /locus_tag="VNN25_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006038532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXH94015.1"
                     /translation="MATASKRKAPKTIDDYIAAYSPDVQAILRKIRTTIRSAAPDAEE
                     TISYRIPAFRQNGILVYFAAFKNHIGLYPPIKADAALEKAIAKYAGEKGNLRFPLDQP
                     IPYALIKRIVTLRVKQNEAKAAAKKAKRKKA"
     gene            1836..3221
                     /gene="gltX"
                     /locus_tag="VNN25_20720"
     CDS             1836..3221
                     /gene="gltX"
                     /locus_tag="VNN25_20720"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXH94016.1"
                     /translation="MTRVRFAPSPTGHLHVGGARTAIFNWLFAKHHGGTFIIRVEDTD
                     QARSTAESETMVLDDLRWLGLLWDEGPDVGGPHAPYRQSERVNRYAEVARELLDRGVV
                     YRCYCTEEELEAKRKEAEVAGRPPHYDLKCWINRRDDEQTPHAMRFHVPDDGDVTIDD
                     LIRGEVTWKRESLGDFILIRSDGHPTYNFSVVVDDHDMEISHVIRAEEHLTNTHRQVL
                     IYRAMEWAVPLFAHVSLILGQDRTKLSKRHGATSVTAYAEAGFLPDAMLNYLTMLGWS
                     APDGRDVFDRQYAIDNFTLDRVNSAPAVFDPQKFEWLNGQYIHAMSAAVLRPLVVHSI
                     GIDTPWLEEAIDVVKTSVHLLPQFREVLRFVTEYNTPSIDQSFASAVAADLREHGAPH
                     DADAVKAMNERLKKATGLKGKELFMPLRLAMTGMDHGPELVRAIPLLARASESDPRVF
                     SPLARVEQCIR"
     gene            3209..4216
                     /locus_tag="VNN25_20725"
     CDS             3209..4216
                     /locus_tag="VNN25_20725"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXH94017.1"
                     /translation="MYSLIRKLLFLLDAEDAHDFTTRQMIALQNIPFALRAIGRFCAP
                     PASARRELLGMAFPSPIGIAAGFDKNGLLMPMLAALGFGFVEVGTVTLRPQPGNPRPR
                     LFRYPEQKALINRLGFNNDGADAVAARLASWQRTIPLFVNIGKNRDVPLEGAADDYGA
                     CAMKLTPLADAVVVNLSSPNTPSLRELQRPEHLERILSAVRGAKPLFVKIAPDLDDAL
                     LNEICEVCRWLANGMICTNTTIARPFTAAEAGGLSGAPLMTQSTSILARVRRRVGPSF
                     PLIGVGGIMKADDLRAKFAAGADLVQVYTGFVYEGPLLASRLARVSDIGSRESEKDYG
                     N"
     gene            4338..5075
                     /gene="rlmB"
                     /locus_tag="VNN25_20730"
     CDS             4338..5075
                     /gene="rlmB"
                     /locus_tag="VNN25_20730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXH94018.1"
                     /translation="MIIYGVNPILEAIRSHSERVRYVGVAREETARHQRLIAEAKRAG
                     VSVRNLASDQIDRLAGRGVHNGVVAEISETAFADFDDVIAEERTTFVLILDSITDPQN
                     FGAILRVADGFGVDLVVIPEHDSVGLTPVAVKASAGASEWVPVAQVTNLARTIETLQK
                     AGYWVYAAADGGDPPQAIDLRGKVALVVGNEGKGIRRNVLEHCDRVVTIPMQGHIESF
                     NVATAAAVLCYEVRRQQGDQKPPRAGR"
     gene            5777..5852
                     /locus_tag="VNN25_20735"
     tRNA            5777..5852
                     /locus_tag="VNN25_20735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5810..5812,aa:Thr,seq:tgt)
     gene            5869..5955
                     /locus_tag="VNN25_20740"
     tRNA            5869..5955
                     /locus_tag="VNN25_20740"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5903..5905,aa:Tyr,seq:gta)
     gene            5975..6050
                     /locus_tag="VNN25_20745"
     tRNA            5975..6050
                     /locus_tag="VNN25_20745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6008..6010,aa:Gly,seq:tcc)
     gene            6130..6204
                     /locus_tag="VNN25_20750"
     tRNA            6130..6204
                     /locus_tag="VNN25_20750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6162..6164,aa:Thr,seq:ggt)
     gene            6238..6399
                     /gene="rpmG"
                     /locus_tag="VNN25_20755"
     CDS             6238..6399
                     /gene="rpmG"
                     /locus_tag="VNN25_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXH94019.1"
                     /translation="MARAGREIITLQCSECKRRNYTTTKNKKTMTEKLELKKFCRHDR
                     KHTPHRETK"
     gene            6511..6586
                     /locus_tag="VNN25_20760"
     tRNA            6511..6586
                     /locus_tag="VNN25_20760"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6544..6546,aa:Trp,seq:cca)
     gene            6681..6929
                     /gene="secE"
                     /locus_tag="VNN25_20765"
     CDS             6681..6929
                     /gene="secE"
                     /locus_tag="VNN25_20765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXH94020.1"
                     /translation="MESDKDNSIVARPREWWLTTRDFFRDTNSEMKKVTWPGRPEVVG
                     TTVVVIVSTIIFAIYLWGCDVIFFKLIDFLFTRFGAGT"
     gene            6932..7525
                     /gene="nusG"
                     /locus_tag="VNN25_20770"
     CDS             6932..7525
                     /gene="nusG"
                     /locus_tag="VNN25_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXH94021.1"
                     /translation="MAETAQKQWYIVHTYSGFEERVRENLNQRIEALGMRGKFGEIKI
                     PTETLIEMKGGKKREVQRKFFPGYILVEMDMADDAWHLVKNTPKVTGFVGTGKKPTPL
                     TPEEVDQILTQVVTTKEKPKPKHVYEHGEHVRIIDGPFSNFTGVVEEVNLDRSTLKVM
                     VTIFGRSTPVELDFLQVARFDPSEEQGGSPTGGAQKR"
     gene            7548..7973
                     /gene="rplK"
                     /locus_tag="VNN25_20775"
     CDS             7548..7973
                     /gene="rplK"
                     /locus_tag="VNN25_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXH94022.1"
                     /translation="MAKKITGYIKLQIPAGAATPAPPVGPALGQHGVNIMEFCKTFNA
                     RTQGQQGLIIPVVITVFSDRTFTFITKTPPAAILLLKAAGIPKGSGVPNKTKVGSVTR
                     KQVEEIAKTKMPDLNAASLEAAVKTVMGTARSMGLEVTG"
     gene            8104..8805
                     /gene="rplA"
                     /locus_tag="VNN25_20780"
     CDS             8104..8805
                     /gene="rplA"
                     /locus_tag="VNN25_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXH94023.1"
                     /translation="MARHGKKYRAAFEKVERRPYQLDEAFGLLKQIAYAKFNETVELS
                     LLLGVDPKHADQMVRGTTVLPHGTGQSKRVLVIAQGDKQREATEAGADMVGGDDIVEK
                     IQGGWTDFDAVIATPDMMRSVGKLGKVLGPRGLMPNPKTGTVTFDVAKAISEIKAGKV
                     EFRVDKTSIIHVPVGKIAFSATQLQENASSIITAIRKAKPPAAKGKYVRSIYISSTMS
                     PSIAIDTAVAEAKAE"
     gene            8815..9420
                     /gene="rplJ"
                     /locus_tag="VNN25_20785"
     CDS             8815..9420
                     /gene="rplJ"
                     /locus_tag="VNN25_20785"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXH94024.1"
                     /translation="MNREDKAAAISELGEGIGKATNAFLMSFKGITVPQVTELRRQVR
                     DTSSTYVVVKNTLALIAVKDSPLTALKGAFSGPTAVAYNTTDAVALAKALTKFAKDVP
                     AITFKGAMLNGQIVAADQIQAIANLPSRNELISKLLYVLQSPMRGLATVLAANIRNLA
                     VVIDQIAKQKGEGAAPAAPEASGAPASEASEAPAPAPEATA"
     gene            9492..9872
                     /gene="rplL"
                     /locus_tag="VNN25_20790"
     CDS             9492..9872
                     /gene="rplL"
                     /locus_tag="VNN25_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXH94025.1"
                     /translation="MAISTEDFVKQIETMSVLDLSNLVKALEDRFGVSAAAAAAPAAA
                     AGPAAAAAPVEAQTEFAVLLTEVGDKKINVIKAVREVTSLGLKEAKDLVEAAPSTIKE
                     AVSKDEAENIKKKFEEAGAKVTIK"
     gene            10254..>11285
                     /locus_tag="VNN25_20795"
     CDS             10254..>11285
                     /locus_tag="VNN25_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXH94026.1"
                     /translation="MNGSSLPKQEPVRHDFSKIRSYMALPNLIDVQRKSYDRFLQMNL
                     LPEEREDTGLQSVFTSVFPFSDFRETCSLDFVKFAIGNWECKCGQLKGLEHLRMTCAN
                     CGSKIITDHPHEETVNCQKCGVINKNRVEICDICGNPVDLQMKYSVEECQERGMSYSV
                     PLKVTFRLFVYDKDPDTGVKHMRDAKEEEVFFGDIPLMTDNGTFIINGTERVIVSQLH
                     RSPGVFFTKESAHTFLSKIIPYRGSWVEFEYDQKNILYVRIDRKRKFLGTIFLRALGL
                     RTDEDILKTFYTVDRIALKNSKLFWQLDPGVERPTNLLGMKVTHSIKSKGGDEIAHSG
                     RKITNAILKE"
BASE COUNT         2351 a         3409 c         3436 g         2089 t
ORIGIN      
        1 gttccggtgc tggacatgct gctcaacgcc gtgaaggaat cgccgggcga tcgcgtgaaa
       61 ttcgtcgccg gcggcgcgac gcggctgcag tcggtggccg cgggactggc cgcggcaggc
      121 gacgagttcg atctcgtggc cattcacgat gcggtgcgtc cgttcttctc gcccgatacg
      181 tttcacgcca cgctcgacgc cgcgcgcacg cacggagcgg cgtttcccgg cattccgctg
      241 cccgacacga ttcatgagat taaagacgac tccatcgctt cgacccccga tcggagtgtc
      301 ttcgtggctg cgcagacgcc gcagtgcttt cgcatcgacg tcctacgtga catcctcacg
      361 cgcgcggcgc gcgaagacgc aaacgcgacc gatgaggcgg ggctggccgc gaagtacggt
      421 caccgcgtcc aactcgttcc cggcgattcg atgaatttca agatcacgcg cccggaggat
      481 ctcgtcatgg ccgagcgcat ctacgcggaa tggggcggag gagaagaatg aagagcccga
      541 gccaattttc gcgcgaagcg ctttggagtg cggtggcgcc agccaccgct ttcaaacgcg
      601 tgcagattag ccgcactgat cgctctcgta ctggaattgc tcgcgccacg tttgtatcga
      661 gcgccttctc cctgcaccaa cggcagcaac gcccccctca tccgccttcg gcaccttctc
      721 cccccgaaaa ggccgggggg aagaaggcgc tcgatacaaa gggatgccta gttctccaag
      781 agtttgagaa atgcgggtta ggcgcaggtc caatgggatt cggacgaaag acgaacatcg
      841 atcggttctg cgcgctgtac gctcttcaaa gcggtggctg gcgccaccgc actccaaagc
      901 gcttcgcgcg aaatgctttt gggggctgcg catgattcgc gtggggcagg ggatcgatgt
      961 tcatcagttc gccgacgcgc gtccgttgat tctcggcggc gtgcgcatca gcgacaccgg
     1021 aggtctctcc ggtcactccg acgccgacgc cgtcctccac gccgtcaccg acgcgctcct
     1081 cggcgctgca ggagcaggcg atatcggcca atacttcccc tccgaggatc cgcgctggaa
     1141 agatgcagat tcgaccatct ttctccgcga ggcgaagcga atcatcgacg agatggatgc
     1201 tgagatcggc aacgtcgacg tcacgatcat cgcgcagacg ccaaagctcg ctccctttcg
     1261 cgacgcgatt caggcctcga tcgcgagttt gctcaatctt cccgcgggcc acgtgaacgt
     1321 caaagcgaca accaccgatc acctcggctt catcggacgc agcgagggca tctgcgcgat
     1381 ggcgatcgtc tcgatatcga tttcctgaac gtccttgaac atggcaaccg cgagcaagag
     1441 gaaggcgcca aagacgatcg acgattacat cgccgcgtat tcgcccgacg tgcaggcgat
     1501 tctccggaag atccgaacga ccatccgcag cgctgcaccc gacgctgagg agacgatcag
     1561 ctatcgcatt ccggcgttca ggcagaacgg gattctggtc tacttcgccg cgttcaagaa
     1621 tcacatcggc ctttacccac ccatcaaggc cgacgcagcg ttggagaagg cgattgccaa
     1681 gtacgcggga gagaagggca atctgcgatt tccgctcgat cagccgattc cgtacgccct
     1741 gatcaagaga atcgtgacgc tgcgggtgaa acagaacgaa gcgaaggcgg ccgcaaagaa
     1801 ggcgaagcga aaaaaggctt gatatcgttg aggacatgac ccgcgttcgt tttgctccat
     1861 cgcccaccgg ccacctccac gtcggcggcg cgcgcaccgc gattttcaac tggcttttcg
     1921 ccaagcatca cggcgggacg ttcatcatcc gcgtcgagga caccgatcag gcgcggtcca
     1981 ccgccgaatc ggaaacgatg gtgctcgacg atcttcgctg gcttggcttg ctttgggatg
     2041 aaggtccgga tgtcggcgga ccgcacgcgc cgtatcgcca gtcggagcgc gtgaacaggt
     2101 acgccgaagt ggcgcgcgag cttctcgatc gcggcgtcgt ctatcgctgc tactgcaccg
     2161 aagaggagct cgaggcgaag cggaaagagg cggaggtcgc agggagaccg ccgcactacg
     2221 acctcaagtg ctggatcaat cgccgtgatg atgagcagac tccccacgcg atgcgttttc
     2281 acgttcctga cgacggcgac gtcaccatcg acgatctgat ccgcggcgag gtgacctgga
     2341 agcgcgagtc gctcggcgat ttcatcctca tccgctctga cggtcatccg acgtacaact
     2401 tttccgtcgt cgtcgacgat cacgacatgg agatctcgca tgtcatccgc gccgaggagc
     2461 atttgacgaa tacgcatcga caggtgctga tttatcgggc catggagtgg gccgttcccc
     2521 ttttcgcgca tgtctcgctg atcctggggc aggaccggac gaagctctcg aaacgtcacg
     2581 gcgccacgtc ggtcactgcg tacgcggaag cgggcttcct tcccgacgcg atgctcaact
     2641 atctgactat gctcggctgg tcggcgcccg acggccgcga tgtcttcgac cgccagtacg
     2701 cgatcgacaa cttcacgctc gaccgcgtga actcggcgcc ggcggtcttc gacccgcaga
     2761 agttcgagtg gctgaacggc caatacattc atgcgatgag tgcggcggta ctgcggccgc
     2821 tggtggtgca ctcgatcggc atcgatacgc cgtggctcga ggaagcgatc gatgtcgtga
     2881 agacgtctgt tcatctcctt ccacagttcc gcgaagtatt gcggttcgtg accgagtaca
     2941 acacgccgtc gatcgatcag tcgtttgcct ctgcggttgc cgccgacctt cgggagcatg
     3001 gcgcgccgca cgacgccgat gccgtcaaag cgatgaacga gcggctgaag aaagccaccg
     3061 gcctcaaagg gaaagagctg ttcatgccgc tgcgcttggc gatgaccggg atggaccacg
     3121 ggccggagct ggtgcgtgcg atcccgctgc tggcgcgggc cagcgaatcg gatccgcgcg
     3181 tgttctcgcc gctcgcgcgc gtcgagcaat gtattcgctg atccgcaagc tgctcttcct
     3241 gctcgacgcc gaggacgcgc acgatttcac gacgcggcag atgatcgcgc tgcagaacat
     3301 ccctttcgcg ttgcgcgcga tcggacgctt ctgcgcgccg cccgccagcg cgcgccgcga
     3361 gctcctcggc atggccttcc catcgccaat cggaatcgcc gcaggcttcg acaagaacgg
     3421 cttgctcatg ccgatgctgg ccgcgctcgg tttcggcttc gtcgaagtgg gcacggtcac
     3481 gctgcgtccg caaccaggca acccgcgtcc gcgcctgttc cgctaccccg aacagaaagc
     3541 gctcatcaat cggctcggat tcaataacga tggagccgat gccgtcgccg cgcggctggc
     3601 atcgtggcag cgaacgatcc ctctcttcgt caacatcgga aagaatcgcg atgttccgct
     3661 cgaaggagca gccgatgact acggagcgtg cgcgatgaag ctcacgccgc tcgcggacgc
     3721 cgttgtcgtc aacctttcct cgcccaacac gccgtcgcta cgcgagctgc agcggcccga
     3781 gcatctcgag cggatactca gtgccgttcg gggtgcgaag ccgctgttcg tgaagatcgc
     3841 gcccgacctc gatgacgctc ttctgaacga gatttgtgag gtgtgccgct ggttggcgaa
     3901 cgggatgatc tgcacgaata cgacgatcgc gcggccgttc acggccgccg aggccggcgg
     3961 actgtcgggt gcgccgctga tgacccaatc gacgtcgatc ctcgcccgtg tgcgcagacg
     4021 cgttggtccg tcgtttccat tgatcggcgt cggcggcatc atgaaggcgg acgaccttcg
     4081 cgcgaaattt gccgcgggcg ccgatctcgt acaggtgtat accggcttcg tctacgaagg
     4141 accgctgctg gcatcgagat tggcgagggt gtcggatatc gggagtcggg agtcggagaa
     4201 ggattacggc aactgatccg accagcgcgc tggagctgga gaaggacttc gctcactgat
     4261 cgattcactg atcgattgac gctctcttct acgactcccg acccccgacc cccgacaccc
     4321 gcactacact tcgcgtcgtg atcatctacg gagtcaatcc catcctcgaa gccattcgct
     4381 cgcactcgga gcgcgtgcgc tacgtcggcg tggcgcgcga ggagacggca cggcatcagc
     4441 ggctcatcgc ggaggcgaag cgggcaggtg tgagcgtgcg caatcttgcc tccgaccaga
     4501 tcgaccgtct agccggtcgc ggcgttcaca acggagtcgt cgcggagatc tcggagacgg
     4561 cgttcgctga tttcgacgat gtcattgccg aggagcggac gacgttcgtg ctgatcctcg
     4621 actcgatcac cgatccgcag aacttcgggg cgatcctgcg tgtcgccgat ggcttcggcg
     4681 tcgatctcgt cgtcatcccg gagcacgaca gcgttggact gacgccggtc gctgtgaaag
     4741 cctcagccgg cgcgtcggag tgggtgccgg tggcgcaggt gaccaacctg gcgcgaacga
     4801 tcgagacgct ccagaaggcg ggctactggg tttacgccgc ggcggatgga ggcgacccgc
     4861 cgcaggcgat cgatcttcgc ggaaaggtgg ctctcgtcgt cggcaacgaa ggaaagggaa
     4921 tccgccgcaa cgtcctcgag cactgcgacc gcgtcgtgac gatcccgatg cagggccaca
     4981 tcgagtcgtt caacgtggcc acggcggcgg cggtgctttg ctacgaggtc cgccggcagc
     5041 aaggggacca gaaaccgcca cgggccggga gatgatgacg aatcaactgg tgaggttcgg
     5101 cagcgtcagg tgtgtcatcg tgggttcgtc cgcatttcca gcaaacaggt aagtgactga
     5161 ttttgaatgg gatatcgttg gtcactcggc atgactgccg tgtcattcgt taatgactcc
     5221 gagtctttcg ctaatgactc agggtcattc gtcaatgact cgggggccat tcgtcaatga
     5281 ctcgggggtc attcgttagt gactcgaggg tctttcgtta aaggctcggg ggtctttcgt
     5341 taaagactcg agggtctttc gcaagagact cgggggtctt tcgctaaaga ctcgggggtc
     5401 tttcgttaaa gactcagggg tctttcgcta aagactcagg ggtctttcgc taaagactcg
     5461 cgggtctttc gcaaaggact cgggggtctt tcgcaaaaga ctcgggggtc tttcgcaaaa
     5521 gactcgggag tctttcgcga gagactcggg ggtctcatgc atgagactcg ggagtccatc
     5581 gcaaggcggc cctttctccc cccccacccc cgacccccca ctcccccgcc aaactgaccc
     5641 catcgaaata agttccctct tgcgcacggt ttggccattt gctataaccc gcagttcctc
     5701 ggcttccgcc gtctgagctc catcctttgc ttggcgtcga caggctgttt gtgtctttga
     5761 aatcttgcag tttggcgctg gcatagctca gttggcagag cagctgattt gtaatcagca
     5821 ggtcgcgggt tcgagtcccg tcgccagctc cagcgtaatc ggtctaacgg agagatggct
     5881 gagtggttaa aagcagcaga ctgtaaatct gccggtcaat gacctacgga ggttcgaacc
     5941 ctcctctctc caccattttt cgcaggaaac gaacgcggga gtaactcagt tggtagagtc
     6001 acagccttcc aagctgttgg tcgcgggttc gagccccgtc tcccgctcca aacgaggttg
     6061 ctgagttgca gggttggttg gtttctgagc aaccccgcaa ccgagcaact tggtatccca
     6121 gaacggtaag ccctcttagc tcaggggtag agcacgtcct tggtaaggac ggggtcgcga
     6181 gttcaactct cgcagagggc tccatcaaaa cgattcgaga tccggagtag agaaaacatg
     6241 gcacgcgcag gcagagagat catcacgctc cagtgttccg agtgcaaacg ccggaactac
     6301 acgacgacga agaacaagaa gaccatgacg gagaagctgg aactgaagaa gttctgccgt
     6361 catgaccgga agcacacgcc gcacagggaa acgaagtaaa gcgaagcacg aatcctgagg
     6421 ccgttggagc ggccgaaggg atgcgggcat ggaaatcgcg ggggaataag tcgctttgag
     6481 cccgcttttg caggcttttg cggcacttgc aggccaatag ctcaactggc agagcagcgg
     6541 tctccaaaac cgcaggttgg gggttcaaat ccctcttggc ctgccaaatc tcagcttcca
     6601 gcgttcagct ctcaaccttc tcaggcggca ccagccggaa gggtgagggc tgaaagctgt
     6661 cagcgttttt cggggtatcg atggaatcag acaaagacaa cagcatcgtc gcaaggccgc
     6721 gcgagtggtg gctcaccaca cgcgactttt ttcgcgacac caactcggaa atgaagaagg
     6781 tcacctggcc gggccgtcct gaagtcgtcg ggacgactgt tgtcgtgatc gtgtccacga
     6841 tcatcttcgc gatctatctc tggggatgcg acgtcatctt cttcaagttg atcgacttcc
     6901 tctttaccag gttcggagca ggcacttagt gatggccgaa acggcacaga agcagtggta
     6961 catcgtccac acctactccg gattcgagga gagggtgcgc gagaacctca accagcgaat
     7021 cgaagccctc ggcatgcgcg gcaagttcgg cgagatcaag atcccgaccg aaacgctcat
     7081 cgagatgaag ggtggcaaga agcgcgaggt gcagcggaag ttcttccccg gctacatcct
     7141 cgtggagatg gacatggcgg acgacgcctg gcatctcgtc aagaacacgc cgaaggtcac
     7201 cggcttcgtc ggcaccggca agaagccgac gccgctgacg cctgaggaag tcgaccagat
     7261 cctgacgcag gtcgtaacga cgaaagagaa gccgaaaccg aagcacgttt acgagcacgg
     7321 cgagcacgtt cgcatcatcg acggaccgtt ctcgaacttc accggagtgg tggaggaagt
     7381 gaacctcgac cgcagcacgt tgaaggtcat ggtcacgatc ttcggccgct ccacgccggt
     7441 cgagctcgac ttcctgcagg tggcccggtt cgatccgagt gaagagcagg gtggcagccc
     7501 aaccggcggc gcacagaaga gataactgat ttcagagacg agaaattatg gctaagaaaa
     7561 ttacgggata catcaagctt cagattccag cgggtgcagc aactcccgca ccacccgtcg
     7621 gtccggccct cggccagcac ggcgtgaaca tcatggagtt ctgcaagacc ttcaacgcac
     7681 gcacgcaggg tcagcagggg ctgatcattc cggtggtcat caccgtcttc tccgaccgca
     7741 ccttcacctt tatcaccaag acgcctcccg cggcgatcct gctgctcaag gcggccggca
     7801 tcccgaaggg ctcgggcgtt ccgaacaaga cgaaggtcgg cagcgtcacg cgcaagcagg
     7861 tcgaggagat cgcgaagacg aagatgccgg atctaaacgc ggcgtcgctc gaagcggccg
     7921 tgaagaccgt gatgggtacg gctcggtcga tgggactcga ggtcaccggc taaaaggcag
     7981 aatgttgaat gctcaacgtt gaacgttgaa cattcggttt gaaaaggtcg cgccggcgcg
     8041 tagccggtgc aaacgaagta ggaggcggga ctcgtgcccg ccgttaacac tacggaggtg
     8101 aatttggcca gacacggcaa gaagtaccgt gcagcgttcg agaaggtcga acggcggccg
     8161 taccagttgg atgaggcatt cggtctgctc aagcagatcg cgtacgccaa gttcaacgag
     8221 acggtcgaac tgtcgctcct tctcggagtc gatcccaagc acgccgatca gatggtccgc
     8281 ggtaccaccg tgcttccgca cgggaccgga cagtcgaagc gcgtgctcgt gatcgcacaa
     8341 ggcgacaaac agcgtgaggc taccgaagcc ggcgctgaca tggtcggcgg agacgacatc
     8401 gtcgagaaga tccagggtgg ctggactgat ttcgacgctg tcatcgccac gccggacatg
     8461 atgcggtcgg tcggtaagct cggcaaagtc ctcggtcccc gcggactgat gccgaatccg
     8521 aagaccggca ccgtcacgtt cgacgtcgcc aaggcgatct cggagatcaa ggccggtaag
     8581 gtggagtttc gcgtcgacaa gacgtcgatc atccacgttc cggttggaaa gatcgcgttt
     8641 tccgcgacgc agctccagga aaacgccagc agcatcatca ccgccattcg caaggccaag
     8701 ccgccggccg cgaagggcaa gtacgtccgc agcatctaca tctcgtccac gatgagcccc
     8761 tcgattgcga tcgatacggc cgtggccgag gcgaaggcag agtaaggagg gagcatgaat
     8821 cgcgaagata aagcagcagc gatctccgag ctcggcgaag gcatcggcaa agccacgaac
     8881 gcattcctga tgtcgttcaa aggcatcacc gttccgcagg tcaccgaact gcgccggcag
     8941 gtgcgcgata cgagctcgac gtacgtcgtc gtcaagaaca ccctcgccct catcgcggtc
     9001 aaggactcgc ctctgacagc gctgaagggt gcattcagcg gcccgaccgc cgtggcctac
     9061 aacaccacgg acgccgtcgc gctggcgaag gcgttgacga agttcgccaa agacgttccg
     9121 gcgatcacgt tcaaaggggc gatgctcaac ggccagatcg tcgcggccga ccagatccag
     9181 gcaatcgcca atctgccgtc gcgcaacgag ctgatctcga agttgctgta cgtacttcag
     9241 agtccgatgc gtggactggc gaccgtactg gcggcgaaca tccgcaatct tgcggtggtc
     9301 atcgatcaga tcgccaagca gaaaggcgaa ggcgcagcac cggctgcccc cgaagcatcc
     9361 ggagcacccg cgtccgaggc atccgaagca cccgcacccg cacccgaggc aaccgcataa
     9421 gactgattcc gcgccgcggc atcgccgcga cgctagagat ttgaacgact caacactatt
     9481 tggaggctga aatggctatt tcgactgaag atttcgtgaa gcagattgag accatgtcgg
     9541 tgctggacct ttcgaacctg gtcaaggcac tggaagaccg cttcggcgtg tcggccgcgg
     9601 cagcagccgc tccggcagca gcagcgggcc cggcagcagc agcggctccg gtcgaggcgc
     9661 agactgagtt tgctgtcctc ctcaccgagg tcggcgacaa gaagatcaac gtcatcaagg
     9721 ctgtacgcga agtgacgagc cttggtctga aggaagcgaa ggacctcgtt gaggccgcgc
     9781 cgtcgacgat caaggaagcg gtctcgaagg atgaggctga gaacatcaag aagaagttcg
     9841 aagaggccgg cgcgaaggtc acgatcaaat agtgacgttt gtggtggttt tcgctggcga
     9901 tctcaacagc cggcggcttt tcgagtatga aagttttccg ggcggggcgg aatgtcccgt
     9961 ccggaatctg ctttttgtta gacatctctg agctggaaag ggggggagcg gccctttccg
    10021 atagctcaca atcctcgcga atccaccctg ccgcgttgcc tgcggcggga ctcgcctaag
    10081 aaattctcat tgagggcgcg tcaatctcga ctaacgcagt gacgaacccg gtgtgcgtgg
    10141 accgcaaccg acggtcgcag gcagagcttt ctgccctgcg gcctcttgct gtctacaccc
    10201 accgcgttcg aaccgccgca gccgggcacg ccgcggccca aaggagcaat tgaatgaacg
    10261 gatcctccct gccaaaacag gaaccggttc gccatgactt ctccaaaatc cggagttaca
    10321 tggcgttgcc gaacctcatc gacgtgcagc gcaagtcgta tgacaggttc ctgcagatga
    10381 acctcctccc cgaggagcga gaagacacgg gactgcagtc ggtcttcacc agtgtcttcc
    10441 ccttcagcga tttccgggag acctgctctc tcgacttcgt gaagttcgcg atcgggaact
    10501 gggagtgcaa gtgcggccag ctcaagggcc tcgagcacct gcgcatgacc tgcgcgaact
    10561 gcggatcgaa gatcatcacc gatcacccgc acgaagagac ggtcaactgc cagaagtgcg
    10621 gcgtcatcaa caagaaccgc gtcgagatct gcgacatctg cggcaatccc gtcgacctgc
    10681 agatgaagta ctcggtcgaa gagtgccagg aacgcggcat gagctacagc gttccgctga
    10741 aggtcacctt ccgcctcttc gtctacgaca aggatcccga taccggcgtc aagcacatgc
    10801 gcgatgccaa ggaagaggaa gtcttcttcg gcgacatccc gctgatgacg gacaacggca
    10861 cgttcatcat caatggaacg gagcgcgtca tcgtgtcgca gctccaccgt tcgccgggcg
    10921 tcttcttcac gaaggaatct gcgcacacgt tcctctcgaa gatcatcccg taccgcggtt
    10981 cgtgggttga gttcgagtac gaccagaaga acatcctcta cgtccgtatc gaccgcaagc
    11041 gcaaattcct gggaaccatc ttcctgcgcg ccctcggatt gcgcaccgac gaagacattt
    11101 tgaagacgtt ctacaccgtg gaccgcattg ccctgaagaa ctccaagctc ttctggcagc
    11161 tcgatcccgg cgtcgagcgc ccgaccaacc tgctcggcat gaaggtcacg cactccatca
    11221 agtcgaaggg tggcgacgag atcgcgcact ccggtcgcaa gatcacgaac gcgatcctga
    11281 aagag
//
LOCUS       DATLVW010000714         5117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_156298, whole genome shotgun sequence.
ACCESSION   DATLVW010000714 DATLVW010000000
VERSION     DATLVW010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156298"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..2373)
                     /locus_tag="VNN25_20800"
     CDS             complement(265..2373)
                     /locus_tag="VNN25_20800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXH94027.1"
                     /translation="MRISVATKLRGVFGLSLVILVVIGSTAIYDTQRLVAVTAARAQA
                     RQFLWDLEQLVANLRGAENEQRGYLLTGEPAYREAYQRSAATLTKSMADLKRRDPSLR
                     SRIDALDPLIAAVIQELNREMDVRDKRGLDAVIADVQDRRSTAAIAAIRSAVTDIEAL
                     ETEVLTARASEAAATAQRTFMTIGFGTLFALIVLSIASVVLSRGITRPVNKLVAGTER
                     IAAGALATRIEAESNDEIGELAHSFNRMAESLETQTRAITDSGGAITDGIRVMTDGSD
                     KLLVRSQGQSALAERSAHSLISVRNITEQVVRSTEQVTANTEDSAARATELRASSEQV
                     RTSMSQLFDSAAKTSSSTTQMSAVAIQMATMTTELSAAGENVLTFVSEMEATTGELGS
                     AARTTADLSRRAREEAVAGRAAVDETVTGIEHSQELTEHAAGVLADLENRVGEIGQIL
                     EVVTAVTHKTNLLALNAAIIAAQAGEHGRGFNVVAEEMRELAEQTRRSTNEISAIVKG
                     VRSASEAAVAAMREGVERVRGTVSLARNASTSLGRIVESSTSSFEMSERISASLQMQA
                     DATRNLHGTASKMSDHLGENRRSINEQAQAARLLAEEAESVHNIAAQVRAASDHQSTA
                     ARGIAEALEQIDIDARTIRDIVRSQLGQIEEVSNASSSMRDIARQNNELAEELSETVR
                     TLARSGERFEKIVGGQRTRA"
     gene            2500..4800
                     /locus_tag="VNN25_20805"
     CDS             2500..4800
                     /locus_tag="VNN25_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXH94028.1"
                     /translation="MTVQAAPNSLERELEYRKKLTGITNQINAAESIPHILMTLKDKI
                     LELLYAERLTIYAVDTKNQELYSLTKVGDVPKEIRVPKSFSSIAGFTALARKTINIKD
                     AYDATELARFHANLRFDQRWDKQTGFRTKAVLAVPILFEKYLLGVVQLINKRHGVAFT
                     PQDEDAAEEIGRILGIAFYNQHRAARQTKPSRLGALVDKGIVSEKDIEAAVSSARVNN
                     STIEKVLIEDYNVPKEEIGKSLAAFYSTSFFSYDGAQTIPAEYRDRVPADMWKKYGCA
                     PIERRPGVLIIAVDDPHDLTRLDGIRAMNLAPRYDFWAGMKHEILAYIGASYGEIEDL
                     ASDSADLNKILDSLGEGEGSAEDEEKDKDEIDETDSGIVKLANKMILEAAARGASDIH
                     VEPDGAKNPCLIRLRVDGDCSKFMEIPGAHRNALVQRLKIMAKLDISERRKPQDGKIR
                     FKSSKGVIELRVATIPTTNGNEDVVMRILAASKPLPIERMGFSERNLKNFKEILAKPY
                     GICLVVGPTGSGKTTTLHSALGSINTEDMKIWTAEDPVEITQKGLRQVQVQPKIDFTF
                     AAAMRSFLRADPDVIMVGEMRDHETAAIGIEASLTGHLVFSTLHTNSAPETISRLLDM
                     NIDPFNFADALLGIMAQRLIRTLCPKCKEPHHPSEEDFDELMEIYGRDYFPQMGIKYS
                     PDFMLYEPKGCANCNHTGYKGRMGVHELLTGTDEVKRAVQRRAPIDELRDLAMQQGMR
                     TLLQDALEKAMRGITDVKQARSIAVK"
BASE COUNT          967 a         1668 c         1535 g          947 t
ORIGIN      
        1 gcgcggacag tacctgctca cctacgtctc cccgagcatc gctccgcgcc ggaaccttcg
       61 ccgcatcagc gtggaagtcc caggcaaacc ggtaatcgtg cgcgccacca gcggctacta
      121 cccgtagaag ggggtcagtc ccaaagtgaa ggcgccctga tttctcacgc gaagacgcga
      181 agtcgcgaag aacgcttagc tgagcatcgc aaacctcgat cgcttcgttt cttcgcgtct
      241 tcgcgtcttc gcgtgagtga cgcgtcaggc tctcgtccgc tgtccgccca cgatcttctc
      301 gaagcgctcg ccgctgcgcg ccagggtccg caccgtttcg gacagctcct cggcaagctc
      361 gttgttctgg cgcgcgatgt cgcgcatcga gcttgatgcg ttcgatacct cttcgatctg
      421 gccgagctgc gatcggacga tgtcgcggat cgttcgtgca tcgatatcga tctgttccag
      481 cgcctcggcg atgccgcggg cggcggtcga ctgatggtcg ctcgctgcgc gcacctgcgc
      541 ggcgatgttg tgtacggact ccgcctcctc ggccagcagc ctcgcggcct gggcctgttc
      601 gttgatggag cggcggttct cgccgaggtg atcgctcatc ttcgatgcgg tgccgtgaag
      661 gtttcgcgtg gcgtcggcct gcatttgaag cgatgcggag atgcgctcgg acatctcgaa
      721 cgatgatgtc gacgactcga cgatgcgccc gagggaggtg ctggcgttgc gggcgaggct
      781 gacggtgccg cggacgcgct ccacgccctc gcgcatcgcc gccaccgcgg cctcggacgc
      841 ggaccgcacg cccttcacga tcgcgctgat ctcgttcgtc gatctgcgcg tctgttccgc
      901 cagctcgcgc atctcctcgg ccaccacgtt gaatccacgc ccgtgctccc cggcttgcgc
      961 cgcgatgatg gcggcgttca gcgcaagaag gttcgtcttg tgcgtgaccg cggtgaccac
     1021 ctcaaggatc tggccgatct cgccgacgcg gttctcgaga tccgccagta cgccggcagc
     1081 gtgctcggtc agctcctgcg agtgctcgat gccggttacg gtctcatcga ccgccgcgcg
     1141 ccccgcgacg gcctcctcgc gcgcgcggcg ggagaggtcg gctgtggtgc gagcagccga
     1201 gccgagctcg ccggttgtcg cttccatctc ggagacgaac gtcagcacgt tctctcccgc
     1261 cgcggacagc tccgtcgtca tcgtcgccat ctggatcgcc actgccgaca tttgcgtcgt
     1321 cgatgatgac gtcttcgccg ccgagtcgaa gagctgcgac atgctcgtgc gcacctgctc
     1381 gctcgaagcc cgcaattccg tggcgcgcgc cgccgaatcc tcggtgttcg cggtgacctg
     1441 ttccgtcgag cgaacgacct gttccgtgat gttgcgtacg ctgatgagcg aatgggcgga
     1501 gcgctccgcg agcgccgact gtccttgcga gcggaccagg agtttgtcgc tgccgtcggt
     1561 catgacccga atgccgtcgg tgatcgcgcc gccgctgtcg gtgatcgcgc gcgtctgcgt
     1621 ttcgagtgac tcggccatcc gattgaacga gtgggcaagc tcgccgatct cgtcgttact
     1681 ctctgcctcg atgcgcgttg ccaatgcacc cgcggcaatg cgctcggtgc ccgccacgag
     1741 cttgttcacg ggacgcgtga tgccgcgcga taacaccacc gaggcgatgg aaaggacgat
     1801 gagggcgaag agagtgccga agccgatcgt catgaacgtt cgctgtgcgg tggctgcggc
     1861 ttcgctcgcg cgtgccgtga gcacttcggt ctccagcgcc tcaatatcgg tgaccgcgct
     1921 gcggatggcg gcaatcgccg cggtactgcg ccggtcctgg acgtcagcga tcactgcatc
     1981 gagacctcgc ttgtcgcgaa cgtccatctc tcgattcagc tcctgaatga cggcagcgat
     2041 gagcggatcg agcgcatcga tgcggctgcg cagcgaggga tcgcgccgtt tgagatcggc
     2101 catgctcttg gtcagcgtcg ccgccgaccg ctgatacgcc tcgcgatagg ccggctcgcc
     2161 ggtgagcaga tagcctcgct gctcattctc cgcgcctcgc aagttggcga cgagctgttc
     2221 gagatcccac aggaactgcc gagcctgggc gcgcgccgcc gtgacggcca ccagccgttg
     2281 tgtgtcgtaa atggcggtcg acccgatgac cacgaggatc accaggctca gcccgaagac
     2341 gccccgcagt tttgtggcaa cggaaatccg catggatggc taggtgcccg tttgtccgca
     2401 acgggcgtgg cgcgcatagt aatgcctcgc ggcgttcttc ggcgtctgac tttccttgat
     2461 tgcgctatcc tttttcttca acttgggaga atcgagacca tgaccgtcca ggctgcgcca
     2521 aattcgctgg agcgcgagct cgagtaccgc aagaaactga cgggaatcac caatcagatc
     2581 aacgccgcgg aatcgattcc gcacatcctg atgacgctca aggacaagat cctcgagctc
     2641 ctctacgccg agcggctcac catctacgcg gtcgacacca agaaccagga gctttactcg
     2701 ctcacgaagg tcggcgacgt ccccaaagag atccgtgtcc cgaagtcgtt ctcgtcgatc
     2761 gccgggttca ccgcgctggc gcggaaaacg atcaacatca aagacgcata tgacgccacc
     2821 gagctggcca ggttccacgc gaatcttcgt ttcgatcagc gttgggacaa gcagaccggc
     2881 ttccgcacga aggccgtcct cgccgtcccc atcctcttcg aaaagtatct cctcggcgtc
     2941 gtgcagctca tcaacaaacg ccacggcgtc gccttcacgc cgcaggatga ggatgccgcc
     3001 gaagagatcg ggcgcatcct cggcatcgct ttctacaacc agcatcgcgc ggcgcggcag
     3061 accaagccct cgcgcctcgg cgcgctcgtc gacaagggca tcgtctcgga gaaggacatc
     3121 gaagccgccg tttccagcgc gcgcgtgaat aactccacga tcgagaaggt gctcatcgag
     3181 gactacaacg tcccgaaaga ggagatcggg aaatcgctgg ctgcgttcta cagcacgagc
     3241 ttcttcagtt acgacggtgc gcagactatt cctgccgagt atcgcgaccg cgtgccggcc
     3301 gacatgtgga aaaagtacgg ctgtgcgccg atcgagcggc gtccgggcgt gctcatcatc
     3361 gctgtcgacg atccgcacga tctgacgcgg ctcgacggta tccgggccat gaacctcgca
     3421 ccgcgctacg acttctgggc cggcatgaag cacgagatcc tcgcgtacat cggtgcgtcg
     3481 tacggcgaga tcgaagacct cgcctcggac agcgccgatc tgaacaagat tctcgattca
     3541 ctcggcgaag gggaggggag cgccgaggat gaggagaagg ataaggacga gatcgacgag
     3601 accgactccg ggatcgtcaa gctcgccaac aagatgatcc tcgaagccgc cgcgcgcggc
     3661 gcctcggaca ttcacgtcga gccggacggc gccaagaatc cctgtctcat ccgcctgcgc
     3721 gtcgacggcg actgcagcaa attcatggag atccccggcg cgcatcgcaa cgcgctcgtg
     3781 caacgcctga agatcatggc caagctcgac atctccgagc gccgcaaacc gcaggatggc
     3841 aagatccgct tcaagtcatc gaaaggtgtg atcgagctgc gcgtcgccac gatcccgacc
     3901 accaacggca acgaagacgt cgtcatgcgc atcctcgccg cctccaagcc gctgccgatc
     3961 gagaggatgg gattcagcga acgcaatctc aagaacttca aagagatcct ggccaagcca
     4021 tacggcatct gcctcgtcgt cggcccgacc ggctccggca agacgaccac cctgcactcg
     4081 gccctcggat cgatcaacac cgaggatatg aagatctgga cggcggaaga cccggtcgaa
     4141 atcacgcaga agggattgcg ccaggtgcag gtgcagccga agatcgactt caccttcgcc
     4201 gcggccatgc gctccttcct gcgagccgat cccgacgtga tcatggttgg cgaaatgcgc
     4261 gatcacgaaa cggcagcaat cggcatcgaa gcgtcgctca ccggccatct cgtcttctcg
     4321 acgctgcaca ccaactccgc gccggaaacg atctcgcgcc tgctggacat gaacatcgat
     4381 cccttcaact tcgccgacgc gctcctcggc atcatggccc agcgcctcat ccgcacgctc
     4441 tgcccgaagt gcaaagaacc gcatcatccc tcggaggaag atttcgacga gctgatggag
     4501 atctacggcc gcgactactt cccgcagatg ggcatcaaat attcacccga cttcatgctc
     4561 tacgagccga aaggctgcgc gaactgcaat cacaccggct acaaaggccg catgggcgtg
     4621 cacgagctgc tcaccggcac cgacgaagtg aagcgcgccg tgcaacgccg cgccccaatc
     4681 gacgagctcc gcgatcttgc catgcagcag gggatgcgaa cactgctaca ggacgcgctg
     4741 gagaaggcga tgcgcgggat caccgacgtg aagcaggcaa gatcaatcgc ggtgaagtga
     4801 aaatcccccg tggttgacgt aaacgccggc tgaaaccgtg gcgtgaaatt actctttgac
     4861 ggcgtgaatt cgcgcaatgt cggtatgaat accaccatgg tagacgtgca tttgctcgaa
     4921 cacctgatga attcctccat ggtagacgtg aatctgctct gatacggcgt gaatctgcgc
     4981 taacccgatt tcctgccgcc tttgtccgtt tcatcaagct ctttgacggc gtgaattcac
     5041 gcagtgtcga tgtgaatacc accatggtag acgtgcatct ggtcaaacac ctgatgaatt
     5101 cctccatggt agacgtg
//
LOCUS       DATLVW010000715         6616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_441044, whole genome shotgun sequence.
ACCESSION   DATLVW010000715 DATLVW010000000
VERSION     DATLVW010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441044"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VNN25_20810"
     CDS             <1..804
                     /locus_tag="VNN25_20810"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP and VCBS repeat-containing protein"
                     /protein_id="HXH94029.1"
                     /translation="LINKNKGKSWQAENIAPPGVKTGADWLSAGDFNEDGRPDFVLGS
                     IYLGSRDIFYLSKGSTEWSVLANDQSRDLIPWGAYFYGNAAGKIASKKRDDAIVSYVR
                     SWPSDLDPNIIARPPLAQTTAIDRLSFAKDGTVTRYPIVRWGSHIPVTGLALADVDGD
                     GNLDIVYAQGTDKELVILLGDGKGGFTRANTEGLKLLGLSIYDLKVVDVNGDGKPDVV
                     IMYESGASTNAASAGGFTAPTFGAQNGSVHVFLNRGVSKAETQARKSSK"
     gene            1169..2779
                     /locus_tag="VNN25_20815"
     CDS             1169..2779
                     /locus_tag="VNN25_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94030.1"
                     /translation="MKKLLVLAMMATLLMAGSAFAVDRHLTPAPDATFQFGAPGVAGG
                     PTTTNNDDSCDIGVAPAATLLLPYFEVDISSARTQDTLFTITNVTRFPQIAHVTVWTD
                     WSFPVLDFNIYLTGYDVQGISMNQVLGGVVAQANGTGPTTAQSPLGALSSGFTNNPNF
                     SGGGATINCSSLPGPLNPTLVAAVRSALTTGVYNPGGSIGCPNPVGGTHANAIGYVTI
                     DVAARCSLALPTDPLYYQNDILFDNVLIGDYQQLGPAAAGTTASSFDAGGNAMVHIRA
                     VPEGGPSGTPLAGMQTNLPYTFYDRYTPSATRTIDRRQPLPALWAARYIQGGTGAFAT
                     NYKIWREGFGSGACGASLASNGAIPVTDIVRFDEHENPNTSTPCNVSPCPSTSTPTMP
                     ETSDQNTSSGTFPTISSADVGGWMYLNLSNGGSLGYSVTRNVGGTPTTVTGGPSALTQ
                     PGGVGPRPSQNWVIVEMFGTAAAGNRLTVDFDAAWLGNGCSAGVGVTGIPANPIAPAG
                     GALVCPPNDPGCVPGSATYPGTNTTPLP"
     gene            2969..>6616
                     /locus_tag="VNN25_20820"
     CDS             2969..>6616
                     /locus_tag="VNN25_20820"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXH94031.1"
                     /translation="MNSIRFRSLLFCVVLLVAATSAVAQNYAFPASTPNTDVKCSLCP
                     GRETLLTIGYPPVLRFVGRWGDAESARDYQQNFRTARPKMARVVPARNRIYTILGSAL
                     AAYDTDKFFSRLNARPQEAMVAETQAVQDTRYPEFGPPEVFLLWDTFFYAETERGWNT
                     PFQDGQERLYDFDWDDRGNVYLAYSVFGWGIVKDSGEMGGRLPSVSQTLGPTPDHILS
                     LKTSDGKYYTAVSDKTQPQLLQIWDVQNASAPVKQPDITGRSFYLWAKDSTGLHVGIV
                     EYGGGLSIFTSDGFVRTGTPLIHFEAGGGGTFRMITSDGTNFYAYGASDIGPFIDVIS
                     PSGNSYVETRYRTNGYGLPQGMHYGDGYLAIYGVELFADSSHPTSAGTWNIRVYKVGP
                     GTLSEIPFEMPVPGSASRQLPFWSMYYSANAPTGYTHPNINNFLDVTPVKNGSKVYLI
                     LSAFGLGDVWELKSSDFLTARLSASAEVPNPNSAAAPGTGPFYGDRQSFTSALGSGNA
                     ANVNWDFGDSTSLASSTGGTVKHQYGGVTSAASLPLVRQVTVTNAGDPTMTDTLPVTL
                     ASPHPRFKLANTSYLFLQPDASSTAPIVSGDSFLDASDGAVEGHYTDWVLDGASNKKV
                     PSEAFGAGACGAHSLAFNTHYGPYSGTGSAITAPSDLSLSISPFNYAVRPYVVTVQEP
                     TPASISDPNAVFTTALRVGGASDLPGGSGTPVTYTWEVIDANQATVLSSNGSATLGTI
                     APFSVPRTTFSALGLRVRLTTTVAASAVPVAGCSSMATAVAQSSVLNGPDPVIVKTGC
                     LTVGTPCSFTVTSAQNPTLAGWSFAWTVNPTATNSGTSASDFKPQFTTTADYTVGVTV
                     TNGIGSKSATLSGHIDKPLCSSAPDNLNTSIGPFVNTTPAPGDAVPFHIFTPGWTPSV
                     ECDKFDWSFGDGGTSTDMFPTHVFNTANTYTVTLKLTGALSTGNYSTTVTIGTPGGGN
                     PGNGGNPGNGGNPGNGGCVAPQSNSAFVAYTGLTSNCTAVAGTCNPGESLQFVLSADS
                     GYNFSCGSGTTVTWSFGDGTGGIGFTPVHTYGSANVYHSQATISNAGGTFTYTRDVQV
                     GNVQPKTCATLTQLNVSLGWSGTTCTEVSGTCKPLDAVAFHAVSTGYDFTCTGTHTYD
                     WDFGDGSSHSTLAQPTHSYLGPGTFNVKMLVGNGTSSTTITRILTVGSGDSGGTGSCG
                     TMTPDNVYVWYD"
BASE COUNT         1274 a         2124 c         1903 g         1315 t
ORIGIN      
        1 ctgatcaaca agaacaaggg aaagtcctgg caggccgaaa acatcgcgcc acccggagtg
       61 aagaccggcg cagactggct ttccgctggt gatttcaacg aagacgggcg gcctgacttc
      121 gttcttggga gcatctatct cggaagccgg gacattttct atctcagcaa aggctcgacc
      181 gaatggtcgg tgcttgccaa tgaccaatcc cgggatctca ttccctgggg tgcctacttt
      241 tatggcaatg cggcgggaaa gatcgcatcg aagaagcggg acgacgcgat cgtcagctac
      301 gttcgttcct ggccgtccga tctcgacccg aacatcatcg cacggccgcc cctggcgcag
      361 acgacggcga tcgaccgtct ttcgtttgca aaggatggca cggtgacccg ttaccccatc
      421 gtgcgttggg gcagccacat tccggtgacc ggactcgcgc tcgccgacgt tgacggcgat
      481 ggcaatctcg acatcgtcta cgcgcagggc acggacaagg agttggtgat tctcctcggc
      541 gacggcaagg gtggattcac gcgcgcgaat accgagggtc tcaagctgct cgggctttcg
      601 atctatgacc tcaaagttgt cgacgtgaac ggcgacggga aaccggatgt cgtcatcatg
      661 tacgagtcgg gcgccagcac gaatgccgcg agtgcgggag gattcacggc gcccacattc
      721 ggagctcaga acggctcggt gcatgttttt ctgaaccgcg gagtttcgaa ggctgagacg
      781 caggctcgga aatcgtcaaa gtgatatcga atgcgcgaag tgttcgagcc ggacaggtcc
      841 cgcgcctgtc cggcgtcgcg agggctgcgg gcatcgtttg cgcattcccg cggcatggcg
      901 attccggatc tgtcctgctg ggcgcagttc ctaaggatgc ttcctgatta ccacctgctt
      961 ctggtacgat ctattgcgat ctatggtgta gcggcataaa aatcacgaaa agggcttgac
     1021 ttcgagtatt ttccgccata ctgttacccg gaactggaat agaacgacaa ttttcaaatt
     1081 cgacgcggga ggtgatcgta tcgtcgagag agggtgaccg cagtccgcaa ctacgatcta
     1141 aacaacatct aatgtgtagg agaacagcat gaaaaaactc ttggttctgg ccatgatggc
     1201 caccctcctg atggcgggct ccgcgttcgc ggtggatcgg cacctgacgc cggctcccga
     1261 cgcgaccttc cagttcggag cgcccggcgt tgccggcggg ccgacaacga cgaacaacga
     1321 cgactcgtgc gacatcggtg tcgcaccggc cgccactctg cttctgccgt actttgaggt
     1381 cgatatcagc agcgcacgta cgcaggacac gcttttcacg atcaccaacg tgacgcgttt
     1441 cccgcagatc gcccacgtga ctgtttggac ggactggtcg tttccggtcc tcgacttcaa
     1501 catctacctg acgggctatg acgttcaggg tatctcgatg aaccaggttc tgggcggcgt
     1561 cgttgcccag gccaacggca ccggtccgac cacagcacag tcgccgctcg gcgccctgtc
     1621 gtccggtttc accaacaacc cgaacttctc cggtggcggc gccaccatca actgctcgag
     1681 ccttccggga ccgcttaacc ccacgctcgt tgcagcggtg agaagcgctc tcaccaccgg
     1741 cgtttacaat ccgggcggtt ccatcggttg cccgaatcct gttggcggca ctcacgcgaa
     1801 tgcaatcggc tacgtcacca tcgacgtcgc ggcgcgttgc tcgctcgctc tgccgaccga
     1861 cccgctttac taccagaacg acattctctt cgacaacgtt ctgattggcg attaccagca
     1921 gctcggaccg gcagcagccg gcacgacggc gtcgtcgttt gatgccggtg gtaacgcaat
     1981 ggttcacatc cgcgccgtac ctgagggcgg tccgtccgga acgccgcttg ccggtatgca
     2041 gacgaacctg ccgtacacgt tctatgaccg ctacaccccg tcggccactc gtacgatcga
     2101 tcgccgccaa ccgcttccgg ctctgtgggc agctcgctac atccagggcg gcacgggtgc
     2161 tttcgccacg aactacaaga tctggcgtga aggcttcggc agcggcgcct gcggtgcatc
     2221 gcttgcttcc aatggtgcca tcccggtaac ggacatcgtg cgtttcgacg agcacgagaa
     2281 tccaaacacg agcactccct gcaacgtttc gccatgcccg tccacgagca cgccgacgat
     2341 gccggaaacg tccgaccaga acacgtcctc aggcaccttc ccgacgatca gctctgccga
     2401 cgtcggtggt tggatgtacc tcaacctgag caacggtggc agcctcggtt actcggttac
     2461 gcgtaacgtc ggtggcactc ccaccaccgt caccggtggt ccgagcgctc tcacgcagcc
     2521 gggcggagta ggcccgcgtc ccagccagaa ctgggtcatc gtcgagatgt tcggaaccgc
     2581 cgccgctggc aaccgcctga ccgtcgactt cgacgcagcg tggcttggca acggctgctc
     2641 ggctggtgtc ggtgtgactg gtattccggc caacccgatc gctcctgccg gtggtgcact
     2701 cgtctgcccg ccgaacgacc caggttgcgt cccgggctcg gctacttacc caggcacgaa
     2761 cacaacgcct ctgccgtaat cagtatcaaa tcgcagttgc tgtgaagcgg gccggatttt
     2821 ccggcccgct tttttttgca aagattcttc gtgacaccga gtcaaaactg agtaaaataa
     2881 caattcactt tcgacagcgt ttcatcgtcg ctggcgaagt cgctcctggt gccgttgcgc
     2941 cattacaact ccttttgggg gaaataacgt gaactcgatt cgcttcaggt cgttgctgtt
     3001 ttgcgttgtg ctgctcgtcg ccgctacgag cgccgtcgcc cagaactacg cgtttccggc
     3061 atcgacgccg aacactgatg tgaagtgctc cctgtgtcca ggccgtgaga cgctcctgac
     3121 gatcggatat cctccggtgc tccggtttgt cggacgttgg ggtgatgccg aaagcgcgag
     3181 ggactatcag cagaacttcc gcacggcgcg tccgaaaatg gctcgcgtcg tccctgcacg
     3241 aaaccgcatc tacacgatcc tcggcagcgc gctggccgca tatgacaccg acaagttttt
     3301 ctcacgcctc aacgccaggc cgcaggaggc catggtggcg gagacccaag ccgtccagga
     3361 cacacggtac ccagagttcg gtccgccgga ggtcttcctt ttgtgggata cgttcttcta
     3421 cgcggagacc gaacgtggct ggaatacccc cttccaggat ggccaggagc ggctctatga
     3481 tttcgactgg gatgaccgcg gcaacgttta tctggcctac tctgttttcg gctggggcat
     3541 cgtcaaggat agcggcgaga tgggtgggcg gcttcccagc gtctcccaga cgctcggccc
     3601 tactcctgat cacatcctgt cgctgaagac gagcgatggc aagtattaca cggccgtcag
     3661 tgacaagact cagccccagc tcctgcagat ctgggacgtt cagaatgcaa gcgcgccagt
     3721 caaacagccc gatatcacgg gccgctcctt ctatctctgg gcgaaagact cgaccggttt
     3781 gcatgtgggg atcgtcgagt acggcggcgg gctttcgatc tttacgtcgg acgggttcgt
     3841 ccgcaccggc acgccgctca tccacttcga ggcgggtggc ggcggcacgt tccgaatgat
     3901 tacgagcgac gggaccaact tctacgctta tggcgcgtca gacattggcc ccttcatcga
     3961 tgtgatctcg ccctccggga actcgtatgt ggagacgcgc tatcgcacaa acggctacgg
     4021 cctgccccag ggaatgcact acggcgacgg ctaccttgcg atttatggag tggagctttt
     4081 cgctgacagc tcgcatccca ccagcgcagg cacatggaac atccgcgttt acaaagtggg
     4141 accgggaacg ttgtccgaga ttccgtttga aatgccggtg ccggggagcg caagccgcca
     4201 actgcctttc tggtcgatgt actactcggc gaacgcaccc acaggctata cacatccgaa
     4261 tatcaacaat ttcctggacg tcacgcccgt caagaatggc tccaaggtct acctcattct
     4321 ctccgcgttc ggtctgggcg acgtatggga gctcaagtcg agtgactttc tcaccgcgcg
     4381 gctgagcgcg tccgctgaag tgcccaatcc gaattcagcc gcggctcccg ggaccggccc
     4441 cttctacggc gaccgccagt cgtttacaag cgcgttgggc tccggaaatg cggcgaacgt
     4501 caactgggac ttcggcgaca gcacgagtct cgcgtcttcc accggcggta ccgtcaagca
     4561 tcagtacggc ggcgtcacgt cggcagcctc gcttcctctg gtccgccagg tgacggtcac
     4621 caacgcgggc gatccgacga tgaccgacac actgccggtg actctcgctt ccccgcatcc
     4681 tcgcttcaag ctcgcgaata cttcatatct gttccttcaa ccggatgcca gctcgaccgc
     4741 gccgatcgtg tccggcgatt cattcctgga tgcgtccgac ggcgcagtcg aaggccacta
     4801 caccgattgg gtgctcgacg gcgcatcgaa caagaaggtg ccgagcgagg cctttggcgc
     4861 aggtgcctgt ggcgcgcact cgctcgcctt caacacgcat tacggcccgt atagcggcac
     4921 cggtagtgcg atcacggcgc ctagcgatct ctccctgagc atctctccgt tcaattacgc
     4981 cgtccgcccc tacgtcgtca cggttcagga accgacacct gcatccatca gcgatccaaa
     5041 cgccgtcttc accaccgctc tccgcgttgg cggtgcgagt gatctgccgg gcggcagcgg
     5101 tacgcccgtg acatatacgt gggaagtaat cgatgcgaac caggcgacgg ttctcagctc
     5161 gaacggttcg gccacgctcg gtacaatcgc gccgttcagc gtgccgcgca ccacgttcag
     5221 tgcgctcgga ttgcgcgtac gtctgacgac cacggtcgcc gcgtcggcag ttcctgtcgc
     5281 cggctgcagc agcatggcca ccgccgttgc acagagcagc gtattgaacg gtccggatcc
     5341 ggtgatcgtg aagaccggct gcttgaccgt tgggactcct tgttccttca ctgtcacctc
     5401 ggcacaaaac ccgacattgg ccggctggtc gttcgcctgg accgtcaacc cgaccgcgac
     5461 caactccggc accagcgcgt cagacttcaa gccgcaattc acgaccacgg cggactacac
     5521 cgtcggcgtc actgttacca acggcatcgg cagcaagtcc gctactcttt caggccacat
     5581 cgacaaaccg ttgtgctcga gcgcgcctga caatctcaac acctccatcg gcccgttcgt
     5641 caacaccact cccgcccctg gcgacgcggt ccccttccac attttcacgc cgggctggac
     5701 gccttcggtt gaatgcgaca agttcgattg gtcgttcggc gatggcggca cgtcgaccga
     5761 tatgttcccg acgcacgtgt tcaatactgc caacacatac accgttacgc tgaagctgac
     5821 aggagcgctt tccacaggca actactcgac cactgtcacc atcggcaccc ccgggggcgg
     5881 aaacccaggg aatggcggaa accctggaaa cggcggaaac ccaggaaacg gcggatgcgt
     5941 tgcgccccaa tccaatagcg cattcgtggc gtacacgggt ctcacatcca attgcacagc
     6001 ggttgccggt acgtgtaacc cgggtgaatc cctgcagttc gttctgtcgg cggactcggg
     6061 ctacaacttc agttgcggta gcggcacgac ggtcacgtgg tccttcggtg acggcacggg
     6121 cggcatcggc tttacgccgg tgcatacgta tggctcggcc aatgtttacc actcgcaggc
     6181 caccatctcc aacgccggcg gcacgtttac gtacacgcgg gacgtgcaag tcggaaatgt
     6241 tcagccgaag acctgcgcca cacttacgca gctgaacgtc tcgctcggct ggtccggcac
     6301 cacctgcacc gaagtcagcg gcacgtgcaa gccgctggac gcggtcgcgt ttcacgctgt
     6361 tagtacgggc tacgacttca cttgcactgg gacccacaca tacgactggg actttggcga
     6421 cggctccagt cactcaaccc ttgcgcagcc aacgcactcc tacctggggc caggcacctt
     6481 caacgtcaag atgttggtcg ggaacggcac ctcgtcgaca acgatcacgc gaatcctgac
     6541 cgtcgggtcg ggcgacagcg gcggtactgg cagttgcgga acgatgactc cggacaacgt
     6601 ctacgtgtgg tacgac
//
LOCUS       DATLVW010000716        17530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_566173, whole genome shotgun sequence.
ACCESSION   DATLVW010000716 DATLVW010000000
VERSION     DATLVW010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17530
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566173"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VNN25_20825"
     CDS             <1..403
                     /locus_tag="VNN25_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94032.1"
                     /translation="DQIEVTTLGDPDLLAADNVKLIVGDPIFVGCALVDANKPAAVVT
                     LSDVKTARWAELVALDEAGNLVRRQTVLRVAERSSEHVIDIQSVKKGVDIEAPLAEIP
                     QRPFDLSRIGTLWLAEPTHRNEPRTVLPPCI"
     gene            complement(428..937)
                     /locus_tag="VNN25_20830"
     CDS             complement(428..937)
                     /locus_tag="VNN25_20830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH94033.1"
                     /translation="MSDLLDQISSYFVDVAPRFALIIVRAQNVDYEIARDALHDAFRA
                     IASRVFAGDLSELADRTRFEKFLYRAASNAAIDVRRRSHRFEKNERTLLLAIDDRQEG
                     AMVTAADTERLHIAIEKLRLPYREIFRRLIYREETLAEIAAALGIPPGSIYKRYSRGV
                     EALRKLLGL"
     gene            complement(934..1248)
                     /locus_tag="VNN25_20835"
     CDS             complement(934..1248)
                     /locus_tag="VNN25_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94034.1"
                     /translation="MAPTVLEQQLLLKGCLEGDPKKLAEFEDRSYLRTIRRVVRKDLE
                     TRLDKENVDHAINLALQAVYRGWKKVPSNGELRERIRSYALAVAEKYYKEWTDQLLRK
                     KP"
     gene            complement(1229..2146)
                     /locus_tag="VNN25_20840"
     CDS             complement(1229..2146)
                     /locus_tag="VNN25_20840"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH94035.1"
                     /translation="MNRINLALGGGGAKGFVHVGVIKALVERGDEIVSIVGTSIGAIV
                     GSIFAYNATIAFKEDPEPQKRAVDALASLLVKEDFWQLLDVNWPSLLRRGIVKGTKIR
                     EWLDDQLVDDGSRSVRFSALPMLTVTATDMMTGECLIVNSENCGATFVSDGVRASMSI
                     QGIFVPAPLDCAGRHVVCWDGGATGNCRFDIARRLGGELTVASSVTYRGEPRFLRTGR
                     IRAALQPLQVADRCADIWLHHIEKMTEELLGEEVMKKIIIVRPDLDGVETMDFGIDER
                     RRRLLIENGRAQTENQLKIFEQTHGADGP"
     gene            complement(2315..3451)
                     /locus_tag="VNN25_20845"
     CDS             complement(2315..3451)
                     /locus_tag="VNN25_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94036.1"
                     /translation="MPRRTVGSVLLAFAVSRGAVILLLIIGSQISFITKEYSTVWRTQ
                     ASVSAGRVLPELSRLAMIGDAWFYRWIAISGYESRTPDGAPKNTWAFFPLYPLLIRAL
                     GGGGPRFPIIAMILSNVALLFALFAVAAAGCALGLADEEAERAAWYIALFPTSYFFSL
                     PMTESIFLLLSAVAFLAAARERWWASGIAGGLASATRVIGICLLPALLLLPCQRSTRL
                     TRRHLWLLLVPLGIAAFVGFLYVRAGDPLAFVHAQTLWGRVSSPTIDLSVLPKGLAIS
                     KPWNFVALNVAAGLLMIAAGVSFLIRRQWSFAAYTLLSVAVPLSSGSVQSLARYALVD
                     FPMFYWLAIVCRTSTRDRAVTAVFVAVLGWMIALFTLRLDFTLA"
     gene            complement(3500..5698)
                     /locus_tag="VNN25_20850"
     CDS             complement(3500..5698)
                     /locus_tag="VNN25_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94037.1"
                     /translation="MPRTMVGVVACLNRSVQRTLCALAVPVALLAATHLSAETIHESA
                     VGITITVERDGHYSVRADDPNWVFSGMLPTPASDLKVANGHDGIGDYHEISFTFGNAR
                     EGSIRTYPRRAAVLFTDRHTTKSANEAGFPTLSTSPAAPFHLSFLGKWSAFQFDLSGT
                     EGPWVSFDNAGHAFILSPASDFMVANLNRTGDDQIESRISPRIATIPQGLAHRTLLVV
                     ANGINRAFDTWGQALTDLNGKTRPANDADPALKSLGYWTDNGAGYYYDWEPSLGYTGT
                     LLAVKDEFAKAGIALGYMQLDSWWYPKGENGDWDRPPEKGPQGILRYEAHPQIFPQGL
                     AAFQKDLGVPLITHARWIDAKSPYRQQYKMSNNVITDPAYWISVGNYLRDAGVFGYEQ
                     DWLDVQATADYKLDDQNAFMDNMALLGNQFGLSLQYCMATPRHFLQTSKYDNITTIRV
                     SEDNFTRDKWDQFFYSSRLASALGVYPFTDVFGSNEKIHLLIATLSAGIVGVGDRIGQ
                     INGDNLLYAVRPDGVIVKPDVPAVPTDESIVNDAHDAGEPLVSFTYTDHGPMRALYVF
                     AHPRTADRTATFTPEALGLGGSVYVYDWIAGKGRKVDGGVPFTQFIHGDPAYLVVVPE
                     GKSGIAFLGDLGKFVPLGKKRVSELSDDGVLEAKIEFASGETSITVEGWSPSAPLFDA
                     AEGRLGVLSFNEQTRRFRVEVFPGKSGSARIRGFVPDPDKLNGPDTGITP"
     gene            complement(5889..7301)
                     /locus_tag="VNN25_20855"
     CDS             complement(5889..7301)
                     /locus_tag="VNN25_20855"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXH94038.1"
                     /translation="MWRSFASRKSASAGVLLLTLICLPAVAQNITGANPVGRRRALVG
                     PANDWTKYCGSLAMDGNADGERVLSPASVPGLQLLWSQKLNGPVASAPSVAYGKVYVG
                     DWAGFEWALDSTSGNAVASADLGTTTSSNCNPATVGISSSPIIEGNVLYLAGGDDGFY
                     ALDVDTLKIIWRRSLGDNSAAGGYYGWCSPTIAGGKVLQGISSNCDNPFPRGALAALD
                     RMTGEVLQTTYFVPEDLVGGGIWTSPAVDTDHRKIFVTTGSAHSFADGDAFSMVRLDL
                     DTLLIEDRWKLPANPDETWDADWGSSPTLFTDAAGRELVGAGQKDGGYYAFDRNNLAA
                     GPLWIAPIAIKGEVPQNGEGTLSTAAFDGTTLYVGGGHPPDSADDSVRGAVTAVSPDD
                     GTILWRHTFAGPVIAPVSFANGVVFAVGGKSVVALDASNGAVLWSYEMKSLGFGGVAI
                     ARGRAFVGDLFGNVYAFGIR"
     gene            8252..10882
                     /locus_tag="VNN25_20860"
     CDS             8252..10882
                     /locus_tag="VNN25_20860"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH94039.1"
                     /translation="MFNRALALERLGLRGEASTAWSAALASDLASPWSAEARDALYRL
                     RASVIQRWSESDVIQASLRRDDRELDRLVIAHPKEARGWGEGASLGKWADAAVKNDKD
                     EARSHLEVARSIGAALKRTSGDQLLISAVDAIAAAERDDRLLRLLARAHIAYRDGRIA
                     FSKQMLQSGESQLREAERLFESAHSPMVFQARYYVASVVYERGGVMEAEGIVDSISKD
                     PQFRNEYSEVGAEVGWIRGLCLVQRGSYAEAIAVFDQARRTFQDLGETERAASLEEFL
                     SDALEVIGDVDEAWRVRRKALERLAGGAYERRRIAAIAEAAERSIANGDWRRAGVVAN
                     IEVDAAEKTSLSDLAVQAHLQRSRVRAATHDRDGAQRDLDKAHRWLASVRDEQMRTRL
                     AIPEAVARAVALSEVDPKQAAAELTAVLVAAERSGAMVDATRLYLERARALRRSGNLA
                     AARSDLSRALDLVEHRRTTLAEARQRVLNLVRGKEVFDEAISLALLEHDSAAAFDLSE
                     RARARSLLEMYARSNEAATPLSSSDIRSLLADDAAVISFSLLGDRITTFVLRHDLLSV
                     YDYADPSVSRISVECARAVQSGGESLAASRCSAVHLSLLGAAERDLVGIKNIAFVSEP
                     ALGELPYAELRDDSGRPLNERFSITESPSATIAIRCAQSLRARGRPVGRAVVVAANEF
                     PPQQDRGLRPLEWVATEARDVAAFYRDSELISGTTATRVRVIDRILVADTFHFAGHAI
                     ASSKRPDQSRLILTASDGGASDLDALAISRLMLNNMRVVYLSACRSGAASHRSDGVDN
                     LALSFIVAGAPTVIATRWDIEDRAAANAARVIHAELARGEDAAAAVARAARLLRKRKS
                     ESDPAWPALAVFGGSRYLIR"
     gene            10939..11790
                     /locus_tag="VNN25_20865"
     CDS             10939..11790
                     /locus_tag="VNN25_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94040.1"
                     /translation="MILALSCNPGRPGNSGSSGTPSPPVVTPTSAGSFVQVDFEGLIA
                     HVVPDTGAAKPRAVVLADDKIHLHAFLIEIPPINEAESELDFIGADHPCVPFAGCSIT
                     SINNGIALTIEGLAPGLTKSTSFKTFVPSLSDAFGTAIPEASLNADLKNPTPTSGDFL
                     AYFDYTGGTLSAVPYCGLGAFKNTGTPRRFVDVVTLIGETTSPAQLKVTNAAGVTKTF
                     TFKDPDYIRILLRNMPINPAAMSDHFPIYAKLFASSAGVKLPEVDHSLACPPKGTVVG
                     CSDSTWP"
     gene            11806..12828
                     /locus_tag="VNN25_20870"
     CDS             11806..12828
                     /locus_tag="VNN25_20870"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXH94041.1"
                     /translation="MKYVAISLLLFAAPFNAAAAVTTTLVKAFSTPANVNRIEREMAV
                     RFANQKYRNRQESVDPDLRELSDEELSELHTIIFHTHTVYGHFDIVPVMRALQQQVQT
                     NSKSVAAIVYKQLLITDPKTGEIHVQAPTLEALGVCPVKDSFTSEPAAATCSGFLVRS
                     DVILTASHCIESPVLGLLRFVFDYLPDNRGGITLSAGSIFEADSVVDRLDSKIGPEWV
                     LVKLKRDTGRTPLTPASTRVAKGDPVSVIGCPGGGTLRSADNATVRDASPSGTFVANL
                     DVFAGNSGSPVFDASGKNVVGILTRGEGDWVDDLSKHCSAMKICPDDQCLGETVTRIS
                     VVPIPK"
     gene            complement(13152..13928)
                     /locus_tag="VNN25_20875"
     CDS             complement(13152..13928)
                     /locus_tag="VNN25_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94042.1"
                     /translation="MNQSDWDAVRDALIADDRATLGEPPSVDELLAYERGELPKEDAE
                     RVQQLLVAYPELARAYATPFPSDDAQPGDSDYLASEVIDRQWNAFHADHRGDAGNRAD
                     HGSTGRVLQFWPSVAAIAAAIAIVFGAMLWRTHTELLRPHVLPEAAAILTPDGRRGGA
                     EPPQVPITPKGDSLLLVVSLVGPTDYDTYRLELVSNDSHKRLWSSEPLRATANNSFSV
                     EFPSRALPSGTYQVIAYGLRGNAQEEVATYTIGIRRPTAP"
     gene            complement(13928..14470)
                     /locus_tag="VNN25_20880"
     CDS             complement(13928..14470)
                     /locus_tag="VNN25_20880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH94043.1"
                     /translation="MIVDGGGPYDERFEAIYRKCYGRVYRYFRTNRIADDEAHDLAQD
                     TFKRLYERMDQIRNDDPWPFLATIAKTVLLNRVRARQTQKRSATMVEIDDPELLFAEP
                     AAPAEPDYADRQAEKARRSRLRAAMASLPRGQRECLRLRMQDFTYDEITTILGISLDA
                     VKSRLRDATRSLREQLGEES"
     gene            14514..>17530
                     /locus_tag="VNN25_20885"
     CDS             14514..>17530
                     /locus_tag="VNN25_20885"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH94044.1"
                     /translation="MKALTVSVALLLVAGACHKEDTGTGSMWGRTIEPRLTTTLSWHS
                     CTKKLSPGHAVQEAQCAAAPIDTGRCDDVIDSREQANRMLVSRPQCTSEAIEALERFS
                     LAEPAAMSDLAGAYYVRAQRKDNPADLLRAFDKAQRAVSLKPQPEGAQFNLALILEAL
                     SMNSDAIEAWQRAALTERGEWADEARAHRSALMQRTAEDGEHQWERIRTQIDSAIDAH
                     DASKTQQLIAIFPGTSQRYFEDDVLAQWAKAPSPQQLSRVSTFAEALTGFTHDRYFTD
                     VAAAIANTRAPDRLRQAQLRYAEARGAQKSINFRSAAPLYAEAARLFQESGSPQCLVA
                     RIGHAEQGALVNDENEAARKELETVEADANRRQYPNVVARVNLNLINTYQFLSRYNEL
                     FAAYETAMTAYGRMGDWEDRAAASSRAIATMSVLGLKDAAWREAFVALRDTPRIVSSK
                     TQHLLIGAAATAALDLDHPEAALLYQNVLVGNARRNGVPTYLVSALDHLAWIELSLRR
                     YGDAQRHVDEAARENDTNAPALRRALDARLAQVKAAAALRIDPGRAVASLTEAIDAAK
                     KPEYATFLAVLFAERAEAFARLGKPADAEADRREALRRLHEEEARVLRDRKPGEDDDR
                     WNFYFSRFEETYDLLIRQLIGEGRVEEAFRVAERARAFEPLDLVRNLPTTPAAFRELA
                     AQPDNLDISRLRALLPPGTLLIEYRVFDDKTYAWIVGRDVFIGQWLTARRTDVKRWTS
                     VLQEAASKRDSAAFEDGLLAPYDGLLRAPLASVNRFRGGAGANIVIVPDRELRGLPFA
                     ALRNPDTRRYAVEDNVLSMSGSALLYVFSVLRDRDLVSGDASALLVGDPAFNPQSTLA
                     RGLQRLSFARKEIDEIRSFYPHPDVLMDGAATPQRFLHLASGSAIIHIAAHGVVNGEA
                     PSQSFLLFNGLLNAQTLMKDLHTDKTRLVVLGACSSAGGLPVGAEGVAPLVRPIIGAG
                     VPGVIGALWDIDDATAEVLLVSFHRHYRQGEDAA"
BASE COUNT         3383 a         5245 c         5510 g         3392 t
ORIGIN      
        1 cgatcagata gaagtcacca ccctcgggga tccagatctg ctcgccgcgg acaatgtgaa
       61 gctcatcgtc ggcgacccaa tcttcgtggg atgcgcgctc gtagacgcca acaagccggc
      121 ggcagtcgtc acactcagcg acgtgaagac ggcgcgctgg gccgagctcg tcgcgctcga
      181 cgaggccggc aatctcgttc ggcgacagac agttctccgt gttgccgagc ggagcagtga
      241 gcatgtcatc gacatccaat ctgtcaagaa aggcgtcgac atcgaagccc cgctcgcgga
      301 gataccccag cgaccgttcg atctcagcag gattggaacc ctttggcttg ctgaaccaac
      361 tcatcgtaat gaaccccgca cagttcttcc cccatgcata tgacgtggag agagaccaaa
      421 cctgacgtta caaccccagt aatttcctca aggcctcaac gccgcgagag taccgcttgt
      481 aaattgagcc cggaggaatg ccgagcgcgg ctgcaatctc ggcgagtgtc tcttcacggt
      541 aaatcagcct ccggaatatc tcgcggtacg gcagcctcag cttctcgata gcgatgtgaa
      601 gccgctcggt atccgccgcc gtgaccatcg ccccctcctg tctgtcgtcg atcgcgagga
      661 ggagagtccg ctcgtttttt tcgaaacggt gagaccgtcg ccgcacgtcg atcgccgcat
      721 tggatgccgc ccggtagaga aacttctcga agcgggtgcg atcggcaagc tcgctgagat
      781 cgccggcaaa cacacggcta gcgattgcgc gaaaggcatc gtgaagtgca tcgcgggcta
      841 tttcgtagtc gacgttttgg gcgcgaacga tgatcagcgc gaagcgtggc gcgacatcga
      901 caaagtaact gctgatctga tcgaggaggt cgctcacggc ttcttccgaa gaagttgatc
      961 tgtccactcc ttgtaatact tttcggcaac ggcgagtgca tacgagcgga tgcgctcgcg
     1021 tagctcgccg ttggagggta cctttttcca gcccctgtag acggcctgca gagccaggtt
     1081 gatcgcgtga tcgacgttct ctttgtcgag ccgcgtctcc aaatctttgc gcacgacgcg
     1141 tctgattgtg cggagatagc tccgatcctc gaactcggcg agcttcttgg gatcgccttc
     1201 gaggcagccc ttcagcagca attgctgttc aaggaccgtc ggcgccatgc gtctgctcga
     1261 agatcttcag ttggttctcg gtttgcgcgc ggccgttttc gatcagcagg cggcgacgcc
     1321 gctcgtctat gccgaagtcc atcgtttcaa cgccatcgag atcggggcgc acgatgatga
     1381 tctttttcat gacctcctcg ccgagtaact cctcggtcat tttctcaatg tgatgaagcc
     1441 agatgtcggc gcatcggtct gcgacttgca gcggttgcaa cgctgccctg atccttccgg
     1501 tgcgaaggaa tcgtggctct cctcgatacg taaccgacga tgcgaccgtg agctctcctc
     1561 ccaggcgcct ggcgatgtcg aatcggcagt taccagtggc gccgccatcc cagcagacga
     1621 cgtgccggcc agcacagtca agcggcgccg gaacgaagat cccttggatc gacatgctcg
     1681 cgcgtactcc atcgctgacg aacgttgcac cgcagttctc gctgttcaca atgaggcatt
     1741 ctccggtcat catgtcggta gccgtgacgg tgagcatcgg tagcgcgcta aaacgaaccg
     1801 aacggctgcc gtcatcgacc agctgatcgt cgagccactc gcggatcttc gttcccttta
     1861 cgatcccgcg gcgaagcagc gatggccagt tcacgtcgag cagctgccag aagtcttcct
     1921 tgacaaggag agaagctaac gcatccactg cccgcttctg cggttcggga tcttctttga
     1981 aggcaatcgt cgcgttgtac gcgaagatcg atccgacaat cgcacctatg ctcgtgccga
     2041 cgatagacac gatttcgtcg ccgcgctcca ccaacgcctt gatcacgccg acgtgcacga
     2101 atccctttgc gccgccaccc ccgagcgcga gattaatgcg gttcacacta ataggagtgt
     2161 cgctgccggc gaaatcccta cctcactact tctttgtgcg ccatccgagt gcgagtgttc
     2221 gtcgaacttt gcctctgcct cgctgccggg caggcttcga acgtcgagaa tttcttgtcg
     2281 cagcatcgct tcgagacgga ttggacattc cgatctacgc cagcgtgaag tcgaggcgca
     2341 gcgtgaatag cgcgatcatc cagccgagga cggcgacgaa gacggctgtc accgcgcggt
     2401 cgcgcgtgga cgtacgacag acgatcgcga gccagtagaa catcgggaag tcgacgagcg
     2461 cgtagcgggc gagcgactgc accgagccgg agctcagcgg cacagcgacc gagagcaatg
     2521 tgtaggcagc aaacgaccac tgccggcgga tcagaaacga aacgcccgca gcgatcatca
     2581 gcagtcctgc tgcaacattc agcgccacga agttccacgg cttgctgatg gcgagaccct
     2641 tgggaagtac cgaaaggtcg atcgtcgggg atgacacccg tccccacaaa gtctgagcgt
     2701 ggacgaaggc cagcggatct cccgcccgaa cgtacaggaa cccgacgaac gccgcgattc
     2761 cgagcgggac gagcagaagc caaaggtgcc gccgtgtcag gcgcgtggag cgctgacaag
     2821 gaagaaggag aagcgcggga aggaggcaga ttccgatgac tctcgtcgcg ctcgcgagtc
     2881 cgcctgcaat cccggaagcc caccagcgct cgcgcgccgc ggcgagaaac gcgacggccg
     2941 agagcagcag gaagatcgac tccgtcatcg gaagcgagaa gaagtagctg gtcgggaaaa
     3001 gcgcgatgta ccaggcggcg cgctccgcct cctcatcggc aagaccgagc gcgcagcccg
     3061 ccgctgccac tgcgaagagc gcgaagagca gcgcaacgtt cgagaggatc atggcgatga
     3121 tcggaaaccg cggccctcca cctccaagcg cgcgaatcag aaggggatag agcggaaaga
     3181 acgcccaggt gttttttggc gcgccgtcgg gcgtgcgcga ctcatagccg ctgatggcga
     3241 tccagcgata gaaccaggca tcgccgatca tggccagccg cgagagctcg ggcaggacgc
     3301 ggccggccga caccgacgcc tgcgtccgcc agacggtgga gtactccttg gtgatgaatg
     3361 aaatctgcga gccgatgatg agaagcagaa tgaccgctcc tcggctgacc gcgaacgcaa
     3421 gaagaacgct gcccaccgtc cgtcgcggca tgagatcgat tctctcatgg gcgctgggtg
     3481 cgcttcggac gatcaggcgt tacggcgtga tcccagtgtc agggccgttg agcttatccg
     3541 gatcgggtac gaatcctcgg attcgcgcgg aaccagactt gccgggaaat acctcgacgc
     3601 ggaaacgtct cgtttgttcg ttgaaggaga gcacgccgag gcgtccttcc gcggcatcga
     3661 aaagaggagc ggaaggcgac catccctcga ccgtgatcga cgtctcgccc gatgcgaact
     3721 cgatcttggc ttcgaggacg ccatcatcgg aaagctcgga cacacgcttc tttccgaggg
     3781 gcacgaattt cccgagatcg cccagaaatg cgatccccga ttttccttcc ggcaccacca
     3841 cgagatacgc cggatcgccg tggatgaatt gggtgaacgg cacgccgccg tcgactttgc
     3901 ggcctttgcc tgcgatccag tcgtacacgt agacgctgcc gccgaggccg agggcctcgg
     3961 gagtaaaggt tgccgtgcgg tccgccgtgc gcggatgcgc aaacacatac agcgcgcgca
     4021 tcgggccgtg atcggtgtaa gtgaatgaga cgagcggttc gcctgcgtcg tgcgcgtcgt
     4081 tcacgatgct ctcgtccgtc ggcaccgccg ggacgtcggg cttgacgatg acaccatccg
     4141 ggcgcacggc gtaaagaaga ttgtctccat tgatctggcc gatgcggtca ccgacgccca
     4201 cgatgcccgc cgagagcgtc gcgatcagca ggtgaatctt ctcgttactt ccgaagacgt
     4261 cagtgaatgg gtagacaccc agtgcggatg ccaggcgcga gctgtagaag aactggtccc
     4321 acttgtcgcg cgtgaagttg tcttcgctca cgcggatcgt ggtgatgttg tcgtacttcg
     4381 aagtctgcag aaagtggcgc ggcgtggcca tgcagtactg caacgaaagg ccgaactggt
     4441 tgcccagcag cgccatgttg tccatgaacg cattctggtc gtcgagcttg taatcagcag
     4501 tggcctgcac gtccagccaa tcctgttcgt agccgaagac gccggcgtcg cgcaggtagt
     4561 tgccgaccga tatccagtag gcgggatcgg tgatgacgtt gttcgacatc ttgtattgct
     4621 ggcgatatgg actctttgca tcgatccatc gcgcgtgcgt gatgagcggc acgccgaggt
     4681 ctttctgaaa cgccgccagg ccttggggga agatttgcgg gtgcgcctcg tagcgcagga
     4741 tcccctgcgg ccccttctca ggcggccgat cccaatcacc gttctccccc ttcggatacc
     4801 accagctatc gagttgcatg tagccgagcg cgatgccggc tttcgcaaac tcgtccttca
     4861 ccgcgaggag cgtgccggtg tatccgagcg acggctccca atcgtagtag tagcccgcgc
     4921 cgttgtcggt ccagtagccg agggatttca aggctggatc ggcatcattg gcgggacgcg
     4981 tttttccatt caggtccgtg agcgcctgcc cccacgtgtc gaacgcgcga ttgattccgt
     5041 tcgccacgac gagcagtgtt cgatgcgcga gtccctgcgg aatcgtggcg atgcgtggac
     5101 tgatgcggct ctcgatctga tcatcgccgg tgcgattcag atttgcgacc atgaagtccg
     5161 aggcgggaga gaggatgaac gcgtgtcccg cgttatcaaa gctcacccac ggtccctcag
     5221 tgccggagag gtcgaactgg aatgccgacc actttccgag gaacgagagg tggaacggcg
     5281 cagcgggtga ggtcgagagc gtcgggaatc ctgcctcgtt cgcggacttc gtcgtatggc
     5341 ggtcggtgaa cagaacggca gcccggcgcg gataggttcg gatcgagcct tcgcgagcgt
     5401 tgccgaacgt gaacgagatc tcgtggtagt cgccgatgcc atcgtgcccg ttcgcgacct
     5461 tcagatccga ggcgggagtg ggaagcatgc cgctgaagac ccagttcgga tcgtcggcgc
     5521 ggaccgaata gtggccatcg cgctcgacgg tgatcgtgat tccaacggcg gactcgtgaa
     5581 tggtctcggc actcaggtgc gtggccgcga gaagagccac gggcaccgcg agcgcgcaaa
     5641 gcgttcgctg aacagacctg ttcagacagg caacgacgcc gaccatcgtg cggggcattg
     5701 gtggactttg gaccgcgcgg atagtagccg aggtcggcgg cagcgtccat tgggagcccc
     5761 tgcgttggag tgcggtcgtc tcgaccgcgg gcactcgggc gtcctcgccc gagtgctctc
     5821 agggcggaga tcgaaattcg tcgacgcttg cgtgaagtgc ggcttcgtag tttctgccgc
     5881 gcgagctcct atcggattcc gaacgcgtaa acgtttccga agagatcgcc gacgaaggcg
     5941 cggccgcgcg cgatggcgac tccgccgaag ccgagggact tcatctcgta tgaccagagg
     6001 acggcgccat tgctcgcgtc gagcgcaacg acggacttgc ctccgaccgc gaagacgacg
     6061 ccgttcgcga acgacacggg ggcgatgacc ggcccggcga acgtatgccg ccagaggatc
     6121 gtgccgtcat ccggcgagac tgcggtcacc gcgccgcgca cggaatcgtc ggcactgtcg
     6181 ggcgggtgtc cccctccgac atagagagtc gtgccgtcga acgcggcagt ggacaacgtc
     6241 ccctcgccat tctgcggaac ctcgcctttg atcgcgatcg gcgcgatcca gagcggtccg
     6301 gcggcgaggt tgttgcgatc gaaggcgtag tagccgccgt ctttctgccc ggcgccgacg
     6361 agctctcgtc ccgccgcatc ggtgaagagg gtgggggaag agccccagtc ggcgtcccat
     6421 gtctcgtcgg gattggccgg cagtttccag cggtcttcga tgagcagcgt gtcgaggtcg
     6481 agtcggacca tcgagaacgc atcgccatcc gcgaacgaat gcgcggagcc ggtggtcacg
     6541 aagattttcc tgtgatcggt atcgaccgcg ggcgacgtcc agatgcctcc gccaacgaga
     6601 tcctcgggga cgaagtacgt cgtctgcaac acctcaccgg tcatgcggtc gagcgccgcc
     6661 agagccccgc gcggaaacgg gttgtcgcag ttgctggaga tgccctgcag aaccttgccg
     6721 cccgcgatcg tgggcgagca ccatccgtaa tagccgccgg ccgcggagtt gtcgccgagc
     6781 gatcgccgcc agatgatctt cagcgtgtcg acgtcgagcg cataaaagcc atcatcgccg
     6841 ccggcaagat aaagaacgtt cccttcgatg atgggcgacg aggaaatccc gaccgtcgcc
     6901 ggattgcaat tgctgctggt ggtcgtgccg aggtccgcag acgcgaccgc gttgccagag
     6961 gtcgaatcga gcgcccattc aaacccagcc caatcgccga cgtagacctt gccgtacgca
     7021 accgagggag ccgaagcaac cggcccattc agtttctgcg accacagcaa ctgcaaccct
     7081 ggaacagatg cgggcgagag gacccgctcc ccatcggcat tgccatccat cgcaagcgac
     7141 ccgcagtact tcgtccagtc attcgccggc cccaccagcg cccgccgcct cccgaccggg
     7201 ttcgctcccg tgatgttctg cgccaccgcg ggaaggcaaa tcaaggtcag caggagcacg
     7261 cccgcgcttg ccgacttcct cgaagcaaag cttcgccaca caatttcctc cgatgcaatc
     7321 cgcgaatcgc gtcatgggac gtcgaaggcc ccagccgcgt cgatttgact caccagtccg
     7381 cttgctaagg ctcagcaagg ttagtagtca ctccaagacg cacttcccgt gccgtgatag
     7441 gcactgtgca gttacagacg ggggaatgtg tggtttgttt acgcaagcaa gccgccgcgt
     7501 gtagcgcagc agtcgccggc gcgctgggtt gctactaacc tcgctgtgac tcccgacata
     7561 catctctcgt tggtcccccg gctgtcggtt tccggggcaa tgtgattgat tgcggcggat
     7621 ttcgatcagt tgcatgttcc gcgagcgcct cctttcttgc cgacgtgata caacgttcat
     7681 caaagccaaa gcgcgtccgt cgtgaaaact cgaaaatgtg tccgtccggc atggacagga
     7741 ggtgcgtgtg cgtctgctaa tggccgtcca acatgccctt gtggtgtcgc tggtattgat
     7801 cgtcctctgt tccatcagat gcggtaagcg agaggatggg tcggagcagg tcaacaggct
     7861 gatcgccgca gcgaagaccg tccgttaccg gtcgacggtg gcacgcctat cagggttctc
     7921 atttcgtcca atacagaaag aacgggggaa ccgccatgat gcagctgacg gcccgtcgct
     7981 caagctagaa agagacgcaa ggcagatcat ccagatgggt gtagccggca ctcgctcgcg
     8041 aggcatcgca tatttgctcg tgggcgatac ggatgcggcg gtggaggacc ttacggaggc
     8101 ggcatcgcga tctggcgatg ctgcgatatg gagtgatctt gccggcgcgc tgatcacgca
     8161 atgcgacgcc ggggatcgcc cgggtgctgc tcttgatggc ttggcggccg ctgatcgggc
     8221 gttggagatt gatccaagat cggaggccgc attgttcaat cgcgcgctgg cgctggagcg
     8281 actaggcctc cgcggcgagg cttcgacagc ctggagtgca gcgctcgcgt cagatctcgc
     8341 gtccccgtgg tcagcggaag cacgtgacgc cctgtaccgg ctccgcgcca gcgtgatcca
     8401 gcgatggagc gagagcgatg tgatccaggc atcgttgcga cgtgatgacc gtgaactcga
     8461 ccggctcgtc atcgcgcatc cgaaggaggc tcgcggctgg ggtgaagggg cgtccctcgg
     8521 caaatgggca gatgcagcag tcaagaatga caaagatgag gctcgatcgc atctggaggt
     8581 cgcgcggagc attggcgccg cgctgaagcg gacatcgggc gatcagttgc ttatctccgc
     8641 ggtcgatgcg atcgcagctg ccgagaggga cgacagacta cttcggctcc ttgctcgcgc
     8701 tcacatcgcg taccgcgacg gacggatcgc gttcagcaag cagatgctgc agtcggggga
     8761 aagccaactc cgcgaagcgg aacggctctt cgaatcggcg cacagtccga tggtttttca
     8821 ggcgcggtat tacgtggcca gcgtcgtcta cgaacgcggg ggagtgatgg aagcggaagg
     8881 gatcgtagac tcgatctcga aagaccctca gtttcggaac gaatacagcg aggttggcgc
     8941 ggaggttgga tggattcgcg gattgtgtct cgtccaacgc ggatcgtacg ccgaggcgat
     9001 tgctgtattc gatcaggcca ggagaacgtt ccaggaccta ggtgagacgg aacgcgcagc
     9061 gtctcttgag gagtttctta gcgatgcgct tgaagtcatc ggagatgttg acgaagcatg
     9121 gcgcgtgcgc cgtaaggcac tcgagaggct cgccggggga gcatacgagc gacggcggat
     9181 agcagcgatt gccgaggcag cggagagaag cattgccaac ggtgactggc ggcgtgcagg
     9241 agtagtcgcg aatatcgagg ttgacgctgc tgaaaagacg tcgctgtccg acctagctgt
     9301 gcaggcgcat cttcaacgca gccgcgttcg agcggcgacg cacgatcgtg acggagctca
     9361 gcgcgatctc gacaaagcgc accgctggct cgcgagcgta agagacgagc agatgcgtac
     9421 gcgcctagcc atcccggagg cggtcgcgcg ggcagtggca ctttctgagg tcgatccgaa
     9481 gcaggcggcg gccgagctca cggcggttct ggtcgcggcc gaacgaagcg gcgcgatggt
     9541 cgatgccaca cgtctctatc tcgagcgggc acgcgcgctc cggcggagcg gcaaccttgc
     9601 cgcggcaagg agtgacctta gccgggcgct cgatctggtc gaacaccgcc gaacgactct
     9661 ggcggaagcc cgacaacgcg tcttgaatct cgtgcgaggg aaggaagtgt tcgacgaagc
     9721 gatttcatta gcgctccttg agcatgattc cgcggcggca ttcgatttgt cagagcgggc
     9781 gcgcgcccgg tcactcctcg agatgtacgc acgctccaat gaagcagcga cgcccctctc
     9841 gtcgtccgat atacgcagtc ttctcgctga cgacgcggcg gtcatctcct tctcgttgct
     9901 tggcgatcgc atcaccacgt tcgtgcttcg ccatgacctc ctgtcggtct atgactacgc
     9961 cgatccgtcc gtcagtcgaa tttctgtgga atgtgcacgg gccgtgcaga gtggcgggga
    10021 atctctagcc gcgagccgct gctctgccgt ccatctgagt ttgctcggag ctgcagagcg
    10081 ggatctcgta ggcatcaaga acatcgcttt tgtgagtgaa ccggcgctcg gcgaattgcc
    10141 gtacgcagaa ttacgcgatg attcgggtcg tccgctcaac gaacgcttct cgatcacaga
    10201 atcgccgagc gccaccatcg cgatccgctg cgcccagagt ctgcgtgcgc gcggccgtcc
    10261 cgtggggcgc gcggtcgttg tcgccgcaaa cgagttcccg cctcagcaag accgtgggct
    10321 gcgcccgctg gaatgggtgg ccactgaagc gcgtgacgta gcagcttttt atcgcgactc
    10381 ggagctaatc tccggcacga ctgcgacacg cgtccgggtt atagaccgaa ttcttgtagc
    10441 tgatacgttc cacttcgctg ggcatgctat tgcgtcgtcc aagcggcccg accagtcccg
    10501 actgatcctt accgccagcg acggcggggc gagtgacctc gacgcactcg cgatctcgcg
    10561 tctcatgctg aacaacatgc gcgtggttta cctgtcggca tgtcggtcgg gagcggcatc
    10621 acacaggtcc gacggtgtcg acaatctcgc cctttcgttc attgtcgcgg gagctccaac
    10681 tgtgatcgca acccgttggg acatcgaaga tcgcgccgcg gcgaatgccg cacgagtcat
    10741 tcatgccgag ctcgcgcgtg gcgaggatgc cgccgccgcg gtcgcgcgtg ctgcgcggct
    10801 actgcgcaaa aggaagtccg agtccgatcc tgcgtggccg gcgctcgccg ttttcggcgg
    10861 atcgaggtat ctaatcagat gagagaggag attgccatgt ttccacccta ctgtaagact
    10921 gtcgcgctca tgctactctt gatcctggca ttgtcgtgca accccggcag gcccggcaac
    10981 tccggcagct ctggtacacc gtcaccgcct gtcgttacac cgacctccgc gggcagcttt
    11041 gttcaggtcg atttcgaagg gctgatcgcg catgttgtac ccgacacggg cgccgcgaaa
    11101 ccgcgtgcag tagtgttggc tgacgacaaa attcacctcc acgcatttct gatcgagatt
    11161 ccgccgatca atgaagcgga gtcggaactc gacttcatcg gggcggatca cccgtgcgtc
    11221 ccttttgccg gctgctccat tacgtccatc aacaacggga tagcgctgac gatcgaaggt
    11281 ctcgctccgg gtctcacgaa atcgacgtca ttcaagacgt ttgttccgag cctgagtgac
    11341 gcctttggta cagcgattcc ggaagcgagc ctcaatgccg acctcaagaa tccaactcca
    11401 acaagcggtg attttctggc atacttcgac tatacgggtg gaacgttgtc ggctgttcct
    11461 tattgcggcc tcggggcatt caaaaatacg ggtacgccgc ggcgctttgt tgacgttgtt
    11521 acactgatcg gcgaaacaac aagtcccgcg caactaaagg taacaaacgc cgctggagtg
    11581 acgaagacgt ttactttcaa agacccggac tacatccgga tcttgctgcg aaacatgcct
    11641 atcaatcccg ctgcaatgtc cgatcacttt ccgatctacg caaagctgtt cgcctcgtcg
    11701 gccggtgtca aactgccaga ggtcgatcac tcgcttgcct gtccaccgaa gggaacggtg
    11761 gtcggttgtt cggattcaac gtggccttga attaggagtg acaccatgaa atacgttgcg
    11821 atttcgttgc tcttgtttgc cgcgcctttc aatgctgcgg ccgctgtcac cacaactctc
    11881 gtcaaggcgt ttagtactcc cgccaacgtc aatcggatcg aacgtgagat ggccgttcgc
    11941 tttgcgaatc aaaaatatcg gaatcgacaa gagtctgtgg atcctgacct tcgagagctc
    12001 tccgatgaag agctttcaga gctgcataca atcattttcc acacacacac cgtatatggc
    12061 cactttgaca ttgtacctgt tatgcgagca ctacagcagc aggtgcagac gaattcgaag
    12121 tcagtcgcgg cgatcgtgta caagcaattg ctgatcacag acccgaagac gggtgaaatc
    12181 cacgtgcaag cgccgaccct tgaagcgctt ggcgtatgcc ctgtcaagga cagtttcaca
    12241 tctgagccgg ccgcggctac atgcagtggg tttctggtca gatccgatgt cattcttacg
    12301 gcatcgcatt gcattgagtc tcccgtactt ggtctcttgc gcttcgtgtt cgactacttg
    12361 cctgacaaca ggggagggat taccttgtcg gcaggcagta ttttcgaggc ggacagtgtt
    12421 gtggaccgtc tcgactcgaa gattggtcca gagtgggtcc tcgtaaagct gaagagggat
    12481 accggacgaa ctccgctcac ccccgcatcg acccgtgttg caaaaggcga tcccgtctcg
    12541 gtaatcggct gtccgggagg aggtactctc agaagtgccg acaacgctac cgttcgtgac
    12601 gcttctccgt cggggacatt cgtggcaaat ctcgatgtgt ttgccggcaa ttccgggtcg
    12661 cctgttttcg atgcttcagg aaaaaacgtg gttggtattc tcacgcgcgg cgagggagac
    12721 tgggtggatg acctttcgaa gcactgcagc gcgatgaaga tttgtcccga tgatcagtgt
    12781 cttggggaaa cagttacccg gatctcggtg gtgcccattc ctaaatagtg aaacgacggc
    12841 gccgccggaa tgatggcggc gccgcagcat cactcacgaa catgctggtg cgggtcaacc
    12901 acactgaaca ccgcatccgg cgatagcgtg gccgcgcatg aggacggacg cggacgggcg
    12961 cctgagaaaa attagagccg aaaggatgag gcagaaggag gagcgaacca ttcggacgat
    13021 tccttggtta cgagtgccaa cgcgacgata tggtcgagtg gtcagttgtt ccggccgcag
    13081 tttgtcgtgt cgattacctt gagtttgtct cgagccagtt cgtcgcgata gtccccattg
    13141 ctttccgcga atcacggagc tgtcgggcgc cggattccga tcgtgtaggt cgcgacctct
    13201 tcttgtgcgt tgccgcgcag gccgtacgcg atcacctggt acgtgccaga tgggagggcg
    13261 cgggatggga actcgacact gaaactgttg ttggccgtcg cgcgcagggg ctcgctcgac
    13321 cagaggcgct tgtgcgaatc gttgctgacc agctccaggc gataggtatc gtagtcggtc
    13381 gggccgacta gcgagacgac gagcaggagc gaatcgccct tgggcgtgat cgggacctgt
    13441 ggcggttctg ctccgcctcg gcggccgtcg ggcgttagaa tggcggctgc ttccggcagg
    13501 acgtgcggcc gtagcagctc tgtgtgcgtg cgccacagca tcgctccgaa gacgatggca
    13561 atagctgcgg cgatcgcggc aacgctgggc cagaactgca gcactcgtcc ggtcgagccg
    13621 tggtcggcgc gattcccggc gtcgccgcgg tggtcggcgt ggaacgcatt ccattgacga
    13681 tcgatgacct cgctggcgag gtaatcagag tcgcccggtt gtgcatcgtc ggacggaaac
    13741 ggggtggcgt acgcgcgggc gagctcggga taggcgacga gcagttgctg cacgcgttcg
    13801 gcatcttctt tggggagctc gccgcgctcg taggcgagaa gctcgtccac gctcggcggc
    13861 tcgccgaggg ttgctcggtc gtcggcgatc aaggcgtcgc gaacggcgtc ccagtcggat
    13921 tggttcatca tgattcttct cccagctgct cgcgaagcga tctcgtagcg tcgcgcagcc
    13981 gcgatttgac ggcatcgagt gagatgccga gaattgttgt gatttcgtcg taggtaaagt
    14041 cttgcatccg caggcggaga cactcgcgtt gaccccgtgg cagcgatgcc atcgcagccc
    14101 ggaggcgcga ccgacgagcc ttttcagcct gccgatccgc gtagtcgggc tctgccggcg
    14161 cagccggctc ggcgaacaac agctccggat cgtcgatctc gaccatcgtc gcgctgcgct
    14221 tctgcgtctg ccgggcacgg acgcggttga gaaggactgt cttcgcgatc gtggccagga
    14281 acggccacgg gtcgtcgttg cgaatctggt ccattcgttc gtagagccgc ttgaacgtgt
    14341 cctgcgcgag gtcgtgcgcc tcgtcgtccg cgatgcgatt ggtgcggaag tagcggtaca
    14401 cgcgcccgta acacttccgg tagattgcct cgaagcgctc gtcgtaggga ccgccgccgt
    14461 ctacaatcat gaccattcgc tcttacccgt catttcgtat ggtccgctca tccgtgaaag
    14521 ccctcactgt atccgttgcg ctgcttttgg tcgccggcgc ttgccataaa gaagacaccg
    14581 gcacgggcag catgtggggg cgaaccatcg agccgcggtt gacgacgacg ctctcgtggc
    14641 actcgtgcac gaagaaatta tcgcccggac atgccgtcca ggaagcgcag tgtgcggctg
    14701 cgccgatcga caccggccga tgcgatgacg tgatcgattc tcgcgagcag gcgaaccgca
    14761 tgctcgtttc gcgtcctcag tgcacaagcg aggcaatcga agcgttggag aggttctcac
    14821 tcgcggagcc ggcggcgatg agcgatctgg ccggcgccta ttacgttcgc gcgcagcgaa
    14881 aagacaatcc ggcggatctg ctgcgcgcgt tcgacaaggc ccagcgcgcc gtgtcgttga
    14941 agccgcagcc ggagggcgcg caattcaatc tcgcgctcat tctcgaggcg ctgtcgatga
    15001 attccgacgc gatcgaggcg tggcagcgcg cggcgttgac ggagcgcggg gaatgggcgg
    15061 acgaggcacg tgcgcaccgg tcggcgttga tgcagaggac ggcggaagac ggcgagcacc
    15121 agtgggagcg cattcggacg cagatcgatt ccgcgatcga tgcgcacgac gcaagtaaga
    15181 cgcagcagct cattgcgatt tttccgggta cttcgcagag atacttcgag gacgacgtac
    15241 ttgcgcaatg ggcgaaagcg ccatcgccac aacagctctc gcgcgtgtcg acgttcgccg
    15301 aggcgctgac aggcttcacg catgaccgct atttcaccga tgtcgctgcg gccatcgcca
    15361 atactcgcgc gccggatcgg cttcggcagg cccaactccg ttatgcggaa gcgcgcgggg
    15421 cacagaaatc gatcaatttc cggtccgcag caccgcttta cgcagaagca gcgcggctct
    15481 tccaggaatc cgggagccct caatgcctcg tggcacgcat cggccatgcc gaacagggcg
    15541 cattagtgaa tgacgagaat gaggccgctc gcaaagagct cgaaactgtc gaagctgatg
    15601 ccaatcgacg tcaatacccc aacgtcgttg cgcgtgtgaa cctgaacctg atcaatacct
    15661 accagtttct cagtcgctac aacgaattgt ttgccgccta tgaaactgcg atgacggcat
    15721 atggccgaat gggagactgg gaagaccgtg ccgcggcgag ctcgcgcgcg atcgccacaa
    15781 tgtcggtgct gggactcaag gatgccgctt ggcgcgaagc gttcgtggcg ctgcgcgaca
    15841 cgccgcggat tgtcagttcg aagacgcagc acctcctcat cggcgcggcc gccaccgcag
    15901 cgctcgacct cgatcatccc gaggcggcac ttctttatca gaatgtgctc gtcggcaatg
    15961 cgcgcaggaa cggcgtgcca acgtatctcg tcagcgcgct cgatcacctt gcatggatcg
    16021 agctctcgtt gcggcgctat ggcgacgcgc agcgccacgt cgacgaggcc gcacgcgaaa
    16081 acgatacgaa cgctcccgct ctccggcgcg cattggacgc tcggctcgct caagtgaagg
    16141 cggcggccgc attgcgtatt gacccgggca gggccgttgc ctcgctgacg gaagccatag
    16201 acgcggcgaa aaagcctgaa tatgcgacat ttctggccgt gttgttcgcc gagcgtgccg
    16261 aggcattcgc ccgcttggga aaaccggccg acgcggaggc cgacaggcgc gaggcactga
    16321 ggcggttgca cgaagaagaa gcgcgggtgc tgcgcgaccg caagccgggc gaggacgatg
    16381 accgatggaa tttctatttc tcccgctttg aagaaacgta cgacctgctg atccggcaat
    16441 tgatcggcga gggccgtgtc gaggaggcat tccgggttgc agagcgtgct cgggcgttcg
    16501 agccactcga cctcgtccgg aatctgccaa ccacgccggc ggcatttcgc gagcttgccg
    16561 cgcaacccga caatcttgat atttccaggt tgcgagcgct tctgccgccc ggaacgcttc
    16621 tcatcgaata tcgggtcttc gacgacaaga catatgcatg gatcgtcggc cgcgacgtct
    16681 tcatcggcca gtggctgaca gcgcgccgca ccgacgtgaa acgctggact tcagtgctac
    16741 aagaggcggc gagcaagagg gattcggccg catttgaaga cgggcttctt gcgccatatg
    16801 acggtctgct tcgagcacct ctggcttcgg tgaatcgctt ccgcggcggc gccggcgcga
    16861 acatcgtcat tgtgccggat cgcgagctgc gcggattgcc atttgccgca ttgcgcaacc
    16921 cggacacgag gcgctatgcc gttgaagaca acgtattgtc gatgtccggt agtgcgctcc
    16981 tgtatgtttt ctctgttctg cgcgaccgcg atctcgtatc gggtgatgct tcggcgcttc
    17041 tcgtcggcga tccggctttc aatccgcaat cgacgctcgc acgaggtctg cagcgcctct
    17101 cgttcgcgcg gaaggagatc gacgagatcc gttcgtttta cccgcatcct gacgtgttaa
    17161 tggacggtgc cgccacgccg cagcgatttc tgcacctggc gagcggaagc gccatcatcc
    17221 acattgcggc gcacggcgtg gtcaacggcg aggcgccttc ccagtcgttc ctcctgttca
    17281 acgggctgct caatgcgcag acgctcatga aggacctgca taccgacaag acacgtctcg
    17341 tcgtcctcgg agcctgcagc agtgcgggag ggctgccagt cggggcggaa ggtgtcgcgc
    17401 ctctcgtgcg tccgatcatc ggcgcaggag ttccgggcgt cattggcgcg ctgtgggata
    17461 tcgacgacgc cacagcggag gtgcttctcg tgtctttcca tcgtcactat cgacagggcg
    17521 aggatgcggc
//
LOCUS       DATLVW010000717         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_279449, whole genome shotgun sequence.
ACCESSION   DATLVW010000717 DATLVW010000000
VERSION     DATLVW010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279449"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..716
                     /locus_tag="VNN25_20890"
     CDS             87..716
                     /locus_tag="VNN25_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94045.1"
                     /translation="MKIAAAILLLAGFPFVAQLTRETNSHAANARGVKQFAAKKYADA
                     QQSFATANGIASSPSRAFNLGTSEIAAGNREAGATTIAKALTDPSLRPDALFNRGNSA
                     LAANAFDYAIRDYSETLRIRPADAAAKRNLEIALRKKQAMQQQQGGNGGKQPNAGQQP
                     QPKPVPQPGDQKKKSDSYEDALLRSVQQQEQEELTRMHKPTRENLHVGW"
     gene            743..2356
                     /locus_tag="VNN25_20895"
     CDS             743..2356
                     /locus_tag="VNN25_20895"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HXH94046.1"
                     /translation="MLREKGVPRRVKHSALAVLCLLLTARLFANDLQVDKHTLSVDDT
                     VTITVSLTDAFASLDSVPFPLQNLVIDGSPSVSSEFSWINGQSTRRKVFTYIAHPRGA
                     GAALIGPVVLRTSDGQVETMAPVSIQVLPDRTSGSNDPVRILHELLATGRDPIFLVAE
                     ADKTSAFVGEQVIVTWTIYNAANVQRQGPGDFPKFSDFWTEELDVRNETLQQVVVGGV
                     PMQKLVIRRVALFPLRSGTLTVEPMSIEGQVMRRSEIGNPFGLFEGTLFDIHRRSAAL
                     TIEARAIPPGPPVATVGDVSMQCQAPQQKSGGPVSIDVVMSGRANLRAAPPPAFARAL
                     DGSMQIAEKGVTVERREGTMTRRWKFLIFPSSSGVFVVPPLVSTILTPAGARRDLRCE
                     ERSVLVEAASAAANPPAATPPPHDARLEAARRSLPAAGVLAAIAILIAITGPRVQRAM
                     RIRRETRSLLRETPAETRAAVDAWLVTSGADPSLLVREQSDRGDAYRALRSLLEAAER
                     DRLTAEPGEIRARVREVIVATSSAPRSNT"
     gene            complement(2378..2674)
                     /locus_tag="VNN25_20900"
     CDS             complement(2378..2674)
                     /locus_tag="VNN25_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94047.1"
                     /translation="MFHTNAGGPGFLPVGALTLAGTKRGRSFANGIVPQGLASLDWYG
                     DSYPTFPDKTHFPLRWLDLNPAISAGNAQRHSGLQMSLIANRSRKHKPASGIHG"
     gene            2706..>3215
                     /locus_tag="VNN25_20905"
     CDS             2706..>3215
                     /locus_tag="VNN25_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXH94048.1"
                     /translation="MSKKLLLSLCAVVLLAISAFGQTADELLEKNLKAMGGKDKLMAL
                     KSVQMTGKMKMGPIEAPFTLTKTRPSKMRVDFTIQGMTGSQAYDGTTGWMVMPFQGNK
                     DPQKMAEEQLKDVRTEADFDGPTFDYKAKGNKVEYVGKEDVQGTPAYKLKVTTKDGKE
                     SNVYLDAETY"
BASE COUNT          633 a          977 c         1044 g          561 t
ORIGIN      
        1 cgcattccga tcgagcgcta ccagtggccg cttgcctttg catggttcgc gttcttcatc
       61 ggctcgctgg ccaatcgagg cgcggaatga aaatcgccgc tgcgattctc ctcctcgcgg
      121 gctttccctt cgtggcccaa ctcactcgcg agaccaattc gcatgcggcc aatgcgcgcg
      181 gtgtgaagca attcgcggcg aagaagtacg ccgatgcgca gcagtcgttc gccacagcca
      241 acggcatcgc ttcctctccc tcacgcgcct tcaacctcgg cacttctgag atcgcggcag
      301 gaaaccgcga ggcgggagcg acgacgatcg caaaggcact cacggatcca tcgctccgtc
      361 ccgacgcgct cttcaaccgc ggcaacagcg cgctggccgc gaatgcgttc gattacgcga
      421 tccgcgatta cagcgagaca ctgcgtatcc ggccagcgga cgccgcggcg aagcgtaacc
      481 tcgagatcgc tcttcgcaag aagcaggcga tgcaacagca gcagggaggc aacgggggca
      541 aacaaccgaa tgccggccag cagccgcaac cgaagccggt gccgcaaccg ggtgaccaaa
      601 agaagaagag cgattcgtat gaggatgctt tgttgcggtc cgtgcaacag caggaacagg
      661 aagagctgac gcggatgcac aagcccaccc gcgagaatct gcatgtgggg tggtgagggt
      721 gagagatgga attgaatgtt ccgtgttgcg tgagaaaggc gttccgcgcc gcgttaagca
      781 ttcggcactt gctgttctct gcttgctcct gaccgcccgg ctgtttgcaa acgaccttca
      841 agtcgacaag cacacgctct ccgtcgatga cactgtgacg attacggtgt cgctcaccga
      901 tgcgttcgcg tcgctcgaca gcgtcccgtt cccgctgcag aacctcgtca tcgacggatc
      961 gccttcggtc tcctcggagt tttcgtggat caacggccag tcgactcggc gaaaagtctt
     1021 cacctacatc gcgcatcccc gaggagcggg agcggcgctg atcgggccgg ttgttttgcg
     1081 cacgagcgac ggacaggtcg agacgatggc gcccgtatcg attcaggtgc ttcctgaccg
     1141 cacgtcaggg agcaacgatc ccgtgcgcat cctgcacgaa ttgctggcga cgggccgcga
     1201 tccgatcttc ctggtggccg aagcggacaa gacgagcgcg tttgtcggcg aacaggtcat
     1261 cgtgacctgg acgatttaca acgcggccaa cgtgcagcga cagggaccgg gcgattttcc
     1321 gaagttcagc gacttctgga ccgaagagct cgacgtgcgc aacgagacct tgcaacaggt
     1381 cgtcgttggc ggtgtgccga tgcagaagct cgtcatccgc cgcgtggcgc tcttcccgtt
     1441 gcgcagcggc acgctgaccg tcgagccgat gtcgatcgaa ggacaggtca tgcggcgctc
     1501 ggagatcgga aaccctttcg ggttgttcga aggaacgctc ttcgacatcc atcgccgctc
     1561 cgccgcgttg acgatcgagg cgcgggcgat tccgcccggc ccgcccgtcg caacggtggg
     1621 cgatgtctcc atgcagtgcc aggcgccgca gcagaagagc ggagggccgg tgtcgatcga
     1681 cgtggtgatg agcggccggg cgaatcttcg cgccgcgccg ccccccgcgt ttgcgcgcgc
     1741 actcgacgga agcatgcaaa tcgccgagaa gggagtgacg gtggagcgcc gcgaaggcac
     1801 gatgacgcgg cggtggaagt ttctgatttt tccatcgagc agtggcgtgt tcgtcgttcc
     1861 tccgctcgtg agcacgatcc tgacgccggc cggtgcgagg cgcgaccttc gctgcgagga
     1921 gcggagcgtg ctcgtagaag cggcgagtgc tgctgccaat ccgccggctg cgacgccacc
     1981 gccgcacgat gcccggctcg aagcggcgcg ccggtcgctg cctgccgcgg gtgtgctcgc
     2041 ggcaatcgcg atcttgatcg ccatcacggg gccgcgcgtg cagcgcgcga tgcgcatccg
     2101 gcgcgaaacg cggtcgctct tgcgcgagac tccggccgag acacgcgccg cggtcgacgc
     2161 ctggctcgtc accagtggcg ccgacccgtc gctcctcgtc cgcgagcagt cggatcgggg
     2221 agacgcttat cgcgcactcc gctcgttgct ggaggctgcg gagcgtgatc gtctcaccgc
     2281 cgagcctggc gagatccggg cgcgggtgcg ggaggtgatt gtggcgacat ccagcgctcc
     2341 gaggtctaat acctgaaccg tgaagcgccg gacgttctca accatggatg ccgctggccg
     2401 gcttgtgctt ccgagaacga ttcgcgatga ggctcatctg gagcccggaa tggcgttgcg
     2461 cattacctgc cgagatggcc gggttgagat cgagccagcg gagcgggaag tgcgtcttgt
     2521 ccggaaaggt gggatacgag tcgccatacc agtcgaggct agcgagccct tgaggaacga
     2581 taccgttcgc gaaactgcgg cctctcttcg tgcccgccag ggttaacgcg ccgacgggca
     2641 gaaaacccgg cccgccggcg ttcgtatgaa acaatcaaaa cgtccaaaca caaccaggag
     2701 gattcatgtc gaagaaactg ctgctttcgc tctgcgccgt ggtgctgctt gcaatctcgg
     2761 cgttcggtca gacggccgat gaattgctgg agaagaacct caaagcgatg ggtgggaagg
     2821 acaagctcat ggcgttgaag tcggtgcaga tgaccggcaa gatgaagatg gggccgatcg
     2881 aggctccctt cacgctcacc aagacgcgtc cgtccaaaat gcgcgttgat ttcacgattc
     2941 agggaatgac cggcagtcag gcatacgacg gtacgaccgg ctggatggtg atgccgttcc
     3001 agggcaacaa agatccacag aagatggctg aagagcagtt gaaggacgtc cgcaccgagg
     3061 ccgactttga tggtcccacc tttgactaca aggctaaggg gaacaaggtc gagtacgtcg
     3121 gaaaagagga tgtacagggc acgccggcct acaagctgaa agtcacgacg aaggacggca
     3181 aagagtcgaa cgtctatctc gacgccgaga cctac
//
LOCUS       DATLVW010000718         4140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_156446, whole genome shotgun sequence.
ACCESSION   DATLVW010000718 DATLVW010000000
VERSION     DATLVW010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156446"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VNN25_20910"
     CDS             complement(<1..354)
                     /locus_tag="VNN25_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94049.1"
                     /translation="MKCCPTCKRPFPPEIELSSRFRQKIYDFIARHPEGVTTMDIMQE
                     IYAERLDGGPESPNVIRVMVSHINAELERRGIEGRLAGSGGRHSVYRWYAPGSNVPRL
                     GRLVKRLKPEVVEAIR"
     gene            complement(351..869)
                     /locus_tag="VNN25_20915"
     CDS             complement(351..869)
                     /locus_tag="VNN25_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94050.1"
                     /translation="MRVYLLLGGLFSIDGYVTSPGMLQLRGMVQENIPGTTFNAYLWG
                     DFLKCHRDLMEHQNDKLAVIGYSGGGHKATWIANGYYGDVRNYLGKPRIDLMVLYDPS
                     PTWSMMQIGNNVKRAICYENLTPWMLGLGGGVLKGPNVTTIPIRMQHLAVQASGTLHR
                     QTLSRIKELGAQ"
     gene            complement(940..1215)
                     /locus_tag="VNN25_20920"
     CDS             complement(940..1215)
                     /locus_tag="VNN25_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94051.1"
                     /translation="MSTTISILGKRQDASNLGADNTAWWPNNIARGMDLCHACFHDLH
                     RRIEGFLEADLCAIAQVRDRIEELENGGMSTVDALRTVHQEIEKART"
     gene            complement(1271..1522)
                     /locus_tag="VNN25_20925"
     CDS             complement(1271..1522)
                     /locus_tag="VNN25_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94052.1"
                     /translation="MNMSPEVLDLCHRLEAVANSSDDLLRQVLHGASCTVGSLLGQRS
                     ANHSAVLDGIDMAHKVIRAAATEVFTYSEKVRAAQKREK"
     gene            complement(1519..1773)
                     /locus_tag="VNN25_20930"
     CDS             complement(1519..1773)
                     /locus_tag="VNN25_20930"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH94053.1"
                     /translation="MTEHEKARAWRQSLGLSQKKLGELLGYSIETISWFEKGMTPPRL
                     HETDRRIKPWIWRRYHLACRGLAAEMKNPRWDWDHEREAV"
     gene            2039..>4140
                     /locus_tag="VNN25_20935"
     CDS             2039..>4140
                     /locus_tag="VNN25_20935"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HXH94054.1"
                     /translation="MPSTNPLLEAALQYAGRGWRVFPLHSVWFKSADTPVCTCYKREA
                     CGTNAGKHPRIAGGFKGATTDRATIEEWWAKWPDANIGIATGNGLTVMDIDGERGRDE
                     LLALLAAKLGDFPSTLAARTGNGGHLYFACEGPRSAAKGHLHVRGEGGYVVAPPSIHR
                     TGRRYEWIDATLAPAVPSDWLRSWLLAGGEAKGLQQRPTGGAEAGVKGGFNRSDSLTH
                     ESGTGTGSLSVQGPNTLVPGPPPAWVAAKLAARTGGRLVHSAAVGTPWTAHEEARLRS
                     ALRSIPASIDGRTWASILMALHDLHWRDGGGADIGLEIADEWSRTSTGRGEGLGLYRG
                     REDLEKRWASFSGRIGNAGSVTVASVYHLAAEHGWKGDVVGVGPEKNGHKIEGLNGHS
                     FAFGQTFGGAGPVGGGPIFIDLDDKGRPKATATNAAQAIEALGVTCRKDTFHEKMLLA
                     GHVIDQWAGDLGDNAVTMLRRVIKREFGFDPGERNARDAAVSLCLEHQFNPVVDYLDG
                     LRWDGVRRIDTWLPHYMNAPDTPFVHAAGRLMLIAAVRRARTPGTKFDQVVVFEGPEG
                     TGKSTAIRYLAGDDNFSDQPILAVGDREQQEAFTGVWLHEIAELEGMRRTDVQRLKQF
                     ISRTEDRARPAYGRIRVDMKRRGIFIGTTNENTYLKSETGNRRFWPIATGSINTDGIK
                     ADRDQLWAEAAYAEESGES"
BASE COUNT          841 a         1195 c         1304 g          800 t
ORIGIN      
        1 gcggatcgct tcgacgacct cgggcttgag ccgcttcacg agcctgccaa gacgtgggac
       61 gttcgagcct ggggcatacc agcggtatac cgagtggcgc ccgccgcttc ctgccagccg
      121 gccctcgatc ccgcgacgtt ccagctcggc gttgatgtgc gagaccatga cgcggatgac
      181 gttgggactc tcggggcccc cgtccaggcg ctcggcgtat atctcctgca tgatgtccat
      241 ggttgtaacg ccttcgggat gtctggcgat aaagtcgtat attttttgcc ggaaccggct
      301 cgacagctcg atctcgggcg ggaaggggcg tttgcaggtg gggcagcact tcattgcgca
      361 cccagttctt ttatgcgcga cagcgtctgt ctgtgcaggg tcccactggc ctggactgcg
      421 agatgctgca ttctgatcgg tatggtcgtg acattcggtc ctttaagaac tccgccaccg
      481 agacccagca tccaaggagt gaggttttca tagcagattg ccctcttcac gttgtttccg
      541 atctgcatca tcgaccaggt cgggcttggg tcatacagga ccatgagatc gatgcgaggc
      601 ttgcccaggt aattccgaac atctccgtag tacccgttgg caatccacgt cgccttatgc
      661 ccacctcccg aatagccgat cacggcgagc ttatcgttct gatgctccat cagatcgcga
      721 tgacatttga ggaaatcgcc ccacaagtac gcgttgaagg ttgttcctgg aatgttttcc
      781 tggaccatcc cccgcagctg gagcatgccg ggacttgtga catagccgtc gatcgagaac
      841 agaccgccca gcagaaggta aactctcatc gcgatacccc atagaaaacg acggaccaga
      901 acagcatggt gctcaggatg acaaggaggg tggaccagat catgttcgcg ccttttctat
      961 ttcctggtga acggtgcgaa gggcgtccac agtagacatg ccgccgtttt cgagttcttc
     1021 gatgcgatca cgcacctgcg ctatagcaca gagatcggct tccagaaaac cttcgatcct
     1081 gcggtgcagg tcgtgaaagc aggcgtggca tagatccatt cctctagcga tattgtttgg
     1141 ccaccacgcc gtattgtcgg cgccgagatt gctggcatcc tgccgcttgc caagaattga
     1201 tatcgtggtc gacatgacca gaccgtcggc cttgcaccga tcgcagcttg tgatcgttct
     1261 gcttgccact ttatttctcc cgcttttgag cggccctgac tttttcgctg tatgtgaaaa
     1321 cctcggttgc cgccgcacga atgactttgt gtgccatatc aataccgtcg agcaccgcac
     1381 tgtggttggc gctgcgttgc ccgagcagac ttccaacagt gcagcttgct ccatggagca
     1441 cctgacgaag aagatcgtcg gaggaattcg ctacagcttc gagtctgtga cacagatcca
     1501 gaacttccgg actcatattc ataccgcctc cctctcgtgg tcccaatccc atcggggatt
     1561 cttcatctca gccgccagac cgcggcaggc aaggtgatag cggcgccaga tccagggctt
     1621 gatcctgcga tcggtctcgt gcaggcgcgg aggcgtcatg cccttctcga accagcttat
     1681 ggtttcaatc gaatagccga gcagttctcc cagctttttc tgactgaggc caagcgattg
     1741 gcgccacgct cgggctttct cgtgttcggt cactggtatt tgttccttga tctgcgatac
     1801 gcaatctcta catatttgta aggaatacgt caacagaccc acgatcacga agtcgtgatg
     1861 gaacaaagcg gattgacatg acggggggat gacgcgcacc ttgccggtcc caaaagggaa
     1921 acgccccggt ggataaggcc ggggcgcctg gggaaatgct gcatgggatc agggaacgac
     1981 agccctgcac acctatgcca gcgcccccgg cgtgtcaagt gaacacaaag ggtagtgcat
     2041 gccatcgaca aacccactgc tggaagcagc gttgcaatac gccggccgcg gctggcgcgt
     2101 attccctctc catagcgtgt ggttcaagtc ggcggatacg ccagtatgca catgctacaa
     2161 gcgcgaagcc tgcggcacca acgccggcaa acatccccga atcgctggcg ggttcaaggg
     2221 cgcgacgacc gatcgcgcga cgatcgagga gtggtgggcg aagtggcctg acgccaacat
     2281 cggtatcgct actggcaacg gtcttaccgt catggacatc gacggcgagc gcggccgcga
     2341 tgaactcctg gcgctgctcg cggccaaact cggtgatttc ccctcgacgc tcgcagcccg
     2401 taccggcaac ggcggacatc tgtactttgc gtgcgaaggt cccagaagtg ccgccaaggg
     2461 ccacctgcac gtccgcggcg agggcggcta tgtcgtcgct ccaccgagca tccatcgcac
     2521 cggcaggcgc tacgagtgga tcgacgcgac gctcgcgccc gctgttccat cagactggtt
     2581 gcggagctgg ctcctggcgg gaggagaagc caagggcctg cagcaacgtc cgacaggcgg
     2641 ggcagaggca ggtgtcaagg gaggatttaa tcggtctgat tcgttaacgc acgaatcagg
     2701 aacgggaacc ggttccctgt ccgttcaggg gcccaatacc ttggtaccag gaccgcctcc
     2761 ggcgtgggtc gcggccaagc tcgcggcgcg gacaggcggg cgcctggtgc atagcgcggc
     2821 cgtcggcacg ccctggacgg cgcacgagga agcgcggctg cggagcgcgt tgcgttccat
     2881 cccagcctcg atcgacggca ggacgtgggc gagcattctc atggcgctgc acgacctgca
     2941 ctggagggat ggaggcgggg cggatatcgg tctggagata gccgacgagt ggtccaggac
     3001 cagcacaggg cgcggcgagg ggctcgggct ctaccgcggc cgggaagacc tggagaagcg
     3061 atgggcgagc ttctcaggga gaatcggtaa cgccgggagc gttactgtgg cttccgtgta
     3121 ccacctcgca gcggagcatg ggtggaaggg cgatgtcgtg ggggttgggc ccgaaaaaaa
     3181 tggccataag attgaagggc ttaacgggca tagttttgca ttcgggcaga cgttcggcgg
     3241 ggcggggccc gtaggcggcg gtcccatctt catcgatctc gacgataagg ggcgccccaa
     3301 ggcgacggcg accaacgccg cgcaagcaat tgaggcgctc ggcgtcacgt gccgcaagga
     3361 tacgttccac gagaagatgc tgctggcggg gcacgtgatc gaccagtggg cgggcgatct
     3421 cggcgacaac gcggtcacga tgctgcggcg cgtgatcaag cgcgaattcg gattcgatcc
     3481 cggcgaacgc aacgcgcggg acgcggcggt gtcgctctgt ctggagcacc agttcaatcc
     3541 agtggtcgat tatctcgacg gattgcggtg ggacggagtg cggcgaattg acacgtggct
     3601 gccgcactac atgaacgcgc cggatactcc attcgtgcac gcggccggga ggctcatgct
     3661 tattgcagcc gtgcggcgcg cgcggacacc cggcaccaag ttcgatcagg tcgtggtgtt
     3721 cgagggaccg gaagggacgg gaaagtcgac ggcgatccga tatctcgcgg gcgacgacaa
     3781 tttcagcgat cagccaattc ttgcagtggg cgaccgggaa cagcaggaag cgttcactgg
     3841 cgtgtggctc cacgagatag cggagctgga aggaatgcgc cgcaccgatg tgcagcgcct
     3901 gaagcaattc atttcgcgca ccgaggatcg ggcgcgaccg gcttacgggc gaatcagggt
     3961 cgacatgaag cgacgcggga tatttatcgg gaccacgaac gaaaacacat atttgaaatc
     4021 ggaaaccggc aacaggcgtt tctggcccat cgccaccggt tccataaata cagacggcat
     4081 aaaagcagat cgcgaccaat tatgggctga ggcggcatac gcggaggaaa gtggagaatc
//
LOCUS       DATLVW010000719        12080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_74311, whole genome shotgun sequence.
ACCESSION   DATLVW010000719 DATLVW010000000
VERSION     DATLVW010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74311"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VNN25_20940"
     CDS             complement(<1..273)
                     /locus_tag="VNN25_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94055.1"
                     /translation="MTNEMVVGIVIAMLAWWAVWLIFMNYVAKTTIPPGHVGWDGEKL
                     YEPGHYKTIYQVQIVDLSPRITTVKSGNRTLIFTWVPDRQNLPAFFR"
     gene            835..1227
                     /locus_tag="VNN25_20945"
     CDS             835..1227
                     /locus_tag="VNN25_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94056.1"
                     /translation="MRKLKPNAVVTLGCGGLLFFATFGILLEVAGKAVSLSNGRHDLV
                     ELDVKPGETSEWVARPKDVDAFAWDYSEALNDYLVAEEEFIDGSRVVSPINTNYDKPI
                     ARVRFTNKGKGIDAIIKVWYRKTIWNGR"
     gene            1427..2326
                     /locus_tag="VNN25_20950"
     CDS             1427..2326
                     /locus_tag="VNN25_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HXH94057.1"
                     /translation="MKRILLPLLLMLCTAPAFAWGQAGHLMTNEAAAIGLPTDMPQFF
                     YRSFPQLTWLGPDPDRWRGGGQSVDASNSPDHQLNYEYVAGLKLPRDRYAFLALLITS
                     GTQRRLGIRNSDPGFLPWRIAEMCEQITTEWRLWRATPASSPERAFIERDIVRDSGVL
                     GHFVADASNPLHTTIAYNGWIWPNPNGYANDCQIHARFETDFVSHSIATENIVAKLAA
                     PQLRADYFDTAIAFVRQSNTLVERTYQLDKKGDFDIFRAPSPEGKTFASERLAAGASL
                     LRDLWWSAWKNSANPAKRRDSGE"
     gene            2455..2781
                     /locus_tag="VNN25_20955"
     CDS             2455..2781
                     /locus_tag="VNN25_20955"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94058.1"
                     /translation="MSALSNSAALSDRRFGYIVWGISNITHEVIGTKVRLHAKKIGNE
                     ELENWLSHQLSPRVNFRIVEEDIDGKPVSLIEVPAASHTPVRFRDHEFRSSGEGARSL
                     EIARTS"
     gene            2723..3769
                     /locus_tag="VNN25_20960"
     CDS             2723..3769
                     /locus_tag="VNN25_20960"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94059.1"
                     /translation="MSSDHPEKERDLWRLLERRDWETEIGETHVRSADLLALLDYPAY
                     FELLQKPLPSERASIINGLADDKLIEFDRLRDTFNITNLGAILFARDLSKFSALSRKQ
                     VRLVVYKGRDRSETIREQGGQRGYASGFAGLVAYLNDLLPANEQIMQAFRTEVRMYPE
                     IAIRELIANALIHQDFRISGTGPIIEVFAERIEITNPGKPLIDTERFLDAPPQSRNEK
                     LGALMRRMKICEERGSGIDKVIKAVEMFQLPAPRFDVTDQHTKVTLYAPQKLSSMERD
                     DRVRACFQHACLRYVSNDTMTNTSLRERFGIAAENYSIASRIIAETIEAGMIKRSDPE
                     SKSNKHAKYVPYWA"
     gene            complement(3936..4472)
                     /locus_tag="VNN25_20965"
     CDS             complement(3936..4472)
                     /locus_tag="VNN25_20965"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXH94060.1"
                     /translation="MKIYTKTGDSGETSLFGGARVRKDDARIEAYGTVDELNSFIGVA
                     RATWPSSSGDAQLHAIQSDLFDIGAHLASPGLSRFDGPDPARIAALEQSIDAMESELA
                     PLKTFILPGGSTAAAHLHVARTICRRAERLVVALRDDDAATVASITYLNRLSDYLFVA
                     ARYANHLYGVPDVPWTRG"
     gene            complement(4521..6032)
                     /locus_tag="VNN25_20970"
     CDS             complement(4521..6032)
                     /locus_tag="VNN25_20970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94061.1"
                     /translation="MAKKILLADDSITIQKVVELTFSDGDYEVTAVNNGAKAIQKLSE
                     MRPDIILSDIIMPEKNGYEVCEYVKSHPEYRNIPVILLTGTFEPFDPDRADKAGCDAV
                     VTKPFESQSLIHKVEELVQQSRSNASAAEPEEAVAPALFADMPSFEEPAPAAAVDPFA
                     HDDDIFSAAPAAASPTTTDMPFDAPPEPAYGNETRALPRMSFEEMQQMANAPSTPAPH
                     QEEAPAPEPSPWDEPAPAFGGETRAFPRMSFEEMEQMASASQPAAETPAETPSPFEAA
                     PSSPAEETSPWDEPKEDAGATRAFPAMSFEEMRQMAEAQATPAPFETQAEVPAPAEPS
                     PWDEAPSSFGGETRAFPRMSFEELEKMAAKPAATPEPEPQPEPEPAWSAPEELPTADP
                     FADTSQAESPFAAEVPEPALAEEAPWTEPAAPEAAAASTEVNSQITDSVTDAGAVPAD
                     LTPDLTDAQVDRIARRVVQLMADKVVRDIAWEVIPDLAEMVVKERIRQLEAEG"
     gene            complement(6115..6312)
                     /locus_tag="VNN25_20975"
     CDS             complement(6115..6312)
                     /locus_tag="VNN25_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008384384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HXH94062.1"
                     /translation="MKTWEYKIINVRSENYSMDPRRAEELNRLGDDGWELVSITSINF
                     KTGATDHIGMVFKREKQQPHA"
     gene            6593..8125
                     /gene="gpmI"
                     /locus_tag="VNN25_20980"
     CDS             6593..8125
                     /gene="gpmI"
                     /locus_tag="VNN25_20980"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXH94063.1"
                     /translation="MPTRPFLILIVIDGFGCRRESEYNAIAQADTPRFDRLYRESAWT
                     TLEASGRAVGLPRDQMGNSEVGHLNIGAGRIVDQDIVRIGKAVARHELEANPTLIDLV
                     RGGGAVHLVGLLSDGGVHSMQTHLHGLIDAAIALGAKEVFVHAILDGRDTPPKSAEKY
                     LERLAEHIKDKPEARIATIIGRYFTMDRDKRWDRVQRGYDLMTLGVGTETKDPIETVR
                     RFYEQNVTDEFIEPISVLTPEGAHKGRMEDGDAVIFFNFRADRMRQIVTGFKDADFDG
                     FHRAVNPKLRIATMNSYREEWTLPVLFPPQEVKNHLGEVLSHAGLKQLRIAETEKYAH
                     VTFFFNGGSDTPSPGEERILVPSPKVATYDLKPEMSIVALADKVVEAVASKKFDVIIM
                     NIANPDMVGHTGVMSAAIEAVHDTDVAVDRILDAVEKIDGIALITADHGNAEFMFDSA
                     TGQPHTAHTTNPVPLILVDSHNRFGKLRSGGSLQNVAPTMLAILGIAQPKEMTGESLL
                     EG"
     gene            8185..10149
                     /locus_tag="VNN25_20985"
     CDS             8185..10149
                     /locus_tag="VNN25_20985"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXH94064.1"
                     /translation="MILEQITDQLTETLTSRVLTLFGHAIDRVVLQSPPRLAMGDLAT
                     PLCLELAKALKRKPRELAEAMVDGLTLPMFVQSVNVEGAGYLNFRFDRGAFTAAHIRS
                     VMTPAAPTGERVIVEHTNINPNKAAHIGHLRNAILGDTYVRCMKWLGYRVETQNYIDD
                     TGVQVADVVVAFQHLEPKSLADVRAMIDDPNVRFDYYCWDVYSKMASYYAAHPEAQQW
                     RRDTLHDIERHEGETFDFASTIARAIVKRHIATMLRLGITYDLLPKESDIIALHFWDR
                     AFELLKASGAVIQETEGKHKDCWVMKLDEASEFEGMNEPDKILVRSNGTVTYTGKDIA
                     YQLWKFGLLDRTFGFRKFWTYPDGHVLWETTSADDGSSDSPHFGGAQRVVNVIDVRQA
                     YLQKVVKEGVRQLGHAREAEKSIHFSYEMVALTPATAKALDIEVSEEDSKKPFIEMSG
                     RKGLGVKADDLIDVLQTKAAESVREGRGEGLTDAEIEQLGHQIAVAALRYFMLKYGRN
                     KVIAFDFNEALTFEGDSGPYLQYSVVRVENIFRKMAERDVDARLDDASLDALTLTEGL
                     TDEMWELVRLSAELPAAIRRATDALELSIITHDLFELAQKFNSFYHKYPILNEKDADE
                     RQRRAVCAEVFRRTMAGALELLGIPVPQRM"
     gene            complement(10228..10449)
                     /locus_tag="VNN25_20990"
     CDS             complement(10228..10449)
                     /locus_tag="VNN25_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HXH94065.1"
                     /translation="MKCRVCGGTQQPTNTDLPFKTGERSIVILKSLPVLQCPQCHEYS
                     IDDPTFRRVEEILAHVDSGAELEIIPFAA"
     gene            10556..10726
                     /locus_tag="VNN25_20995"
     CDS             10556..10726
                     /locus_tag="VNN25_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94066.1"
                     /translation="MKLDLQDIALRTLILVAGGLAALVLSLKGQGQTLPALAVGGTLG
                     AFMMARFGPTQH"
     gene            complement(10736..10960)
                     /locus_tag="VNN25_21000"
     CDS             complement(10736..10960)
                     /locus_tag="VNN25_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXH94067.1"
                     /translation="MAEIGTVKWFNNDKGFGFIKRDTGEDVFVHHSAIQAQGYRTLNE
                     GERVEFDVKQGPKGLQAENVHRAEGERNAS"
     gene            complement(11054..11917)
                     /locus_tag="VNN25_21005"
     CDS             complement(11054..11917)
                     /locus_tag="VNN25_21005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH94068.1"
                     /translation="MATLPVEGRWSIKSVGRDNIAETLAFLRRDPLINVYLIARINEE
                     RFASSTQTVEVRHNREIVLVASLATNIVLAGDRALSPDLTETAIAAIADRILTKMLPV
                     RAIISPAHLVEQLWARLHNRLDPPTVVRMNQPVYAIRGRLDFPDLSEARYATTRDLEA
                     LVPACASMHIEEVGINPLERDSAGYRERVRELVEQKRSVIRIVDGMIAAKCEYSAVTD
                     DMVQLMGVWTNPRLRRHGHARALLREVCGHLARKGKTVTLFVNDFNLPAIALYESLGY
                     QRIGMNRALIW"
BASE COUNT         2410 a         3598 c         3672 g         2400 t
ORIGIN      
        1 ccgaaaaaac gctggaaggt tttgccgatc gggaacccaa gtaaaaatca acgtccgatt
       61 gccggatttc acggtcgtga ttctgggaga caaatcaacg atctggactt ggtaaatcgt
      121 tttgtagtga ccgggttcgt agagcttctc gccgtcccat ccaacgtgtc ccggcgggat
      181 ggtggtcttc gccacgtagt tcatgaagat gagccagacg gcccaccaag cgagcatggc
      241 tatgactatc ccgaccacca tctcattcgt cacaggtgcc tcttttcccc cgtgcccgat
      301 agggatagga gcggtaggac gataggttgg aagcaaatac gcgggactcg ccccctccga
      361 tgggagaggg acggttggcg aaccaggagc cgttagccgt cgccgttagg cgtcgtattc
      421 tcaaccagtt aaaaaggacg cgccgagtgt ccggcgctat ccgtactaca accggggttc
      481 ctactagcgt aggtaggtta tgggatacgt accgctgagc gattctctat gcgaagggca
      541 acccaacctg gaatccgaaa gacccgatac ggtcgcttgc cggtgtcctg catcttgaag
      601 attagaacgc ccgactcttc ttcgaccagt cgcgttaccg attctctcga aaggttccag
      661 agttgtgaaa gttccttcac gctgtacgtg tgattcgcat cgagtgaata gaggtgcttc
      721 gtttcttcca ccattccaag cctccgtatt cgggaggttt gtgtccccgc gcccgatgcg
      781 agcgggtagg tcaaagagca cttttgtccg gtcggatgat accatcgagg ctccgtgaga
      841 aagctgaaac cgaacgccgt ggtgacgctc ggatgcgggg gtcttctctt ctttgcgacg
      901 ttcgggattc tcctcgaagt ggcggggaag gcggtgtcgc tctcgaacgg ccggcacgac
      961 ctcgtggagt tggacgtgaa gccaggggag acttcggagt gggtcgctcg cccaaaagat
     1021 gtggacgcat tcgcctggga ctactcggag gcacttaatg actaccttgt tgccgaggag
     1081 gagttcattg atggttcgcg ggtcgtgtct ccgatcaaca cgaactacga caagccgatt
     1141 gcgcgagtga gatttacgaa caagggcaag ggtatcgacg cgataatcaa agtctggtac
     1201 cggaagacga tatggaatgg acggtagcgc tttcgtgtac ttgatttgta cctagcccgc
     1261 cggtttttgg aacagataga gcttctggag tgttatagaa tcagtaagtt acagaaacca
     1321 cggggacgcg aaaagtacaa atcctcttgc cccgaccatc tggaaatgat tgcggcctcg
     1381 caccctgcga ggccgtttcc ttttttgcct caactacaat cggccggtga aacgaatcct
     1441 cctccctctt ctgctcatgc tttgcaccgc tcccgcgttt gcgtgggggc aggccgggca
     1501 cctgatgacc aacgaggcgg ccgcgatcgg attgccgacc gacatgccac agttcttcta
     1561 tcgatcgttc ccgcagctca cctggctggg tcctgatccc gaccgctggc gcggaggtgg
     1621 tcagtcggtc gatgcgtcga acagtccgga tcaccagctc aactacgagt acgttgccgg
     1681 gcttaaactg ccgcgcgacc gttacgcgtt tctcgcgctc ctcatcacct cgggcacgca
     1741 gcgccgcctc ggcatccgga actcggatcc cggcttcctg ccgtggcgca tcgccgagat
     1801 gtgcgagcag atcacgactg aatggcgtct ctggcgcgcc acgcctgcca gttcaccgga
     1861 gcgcgcgttc atcgagcgcg acatcgttcg cgactccggc gttctcgggc acttcgtcgc
     1921 ggacgcctcg aatccgttgc atacgacgat cgcctacaac ggctggatct ggccgaaccc
     1981 gaacggctac gccaacgatt gccagatcca cgcgcgcttc gaaactgact tcgtatcgca
     2041 ctcgatcgcc acggaaaaca tcgtggcgaa gctggcggct ccgcagttgc gcgccgacta
     2101 cttcgacacg gcgatcgcgt ttgtgcggca atcgaacact ctggtcgagc ggacatacca
     2161 actcgacaaa aagggagact tcgacatctt ccgcgctcct tcacccgagg gaaagacatt
     2221 cgccagcgag cggctggctg cgggcgcgtc gctgttacgc gatctctggt ggtcggcgtg
     2281 gaagaactcc gcgaatccag cgaaacggcg ggattcgggg gagtagcgcc gacgcccgcg
     2341 tgagatcatc ctgatctgtc caatctgctc gcgcacctca ctgcggcgaa cagagaaacc
     2401 gaatggatcg agttcaagct gaatgacgat acgcccgagg aaatcggcga gtacatctcg
     2461 gccctatcca actccgcagc gctgtcggac aggcggtttg gctacatcgt atgggggatc
     2521 agcaacataa cgcatgaggt catcggcacg aaggtacggt tgcacgcaaa gaagattgga
     2581 aacgaagagc ttgaaaactg gctctcgcat caactctcgc caagagtcaa cttccggatt
     2641 gtggaggagg atattgacgg gaaaccggtt tcgctaatcg aagtgcctgc cgcgtcacac
     2701 actccggtca ggtttcgtga tcatgagttc agatcatccg gagaaggagc gcgatctctg
     2761 gagattgctc gaacgtcgtg actgggaaac cgagatcggc gagacgcacg ttcgaagcgc
     2821 tgatctcctc gcattgctcg actaccccgc ttacttcgag cttcttcaga agccgctacc
     2881 ttcggaacgg gcgtcgatca tcaacggcct tgcggacgat aagctgatcg aattcgatcg
     2941 cctccgagat acgttcaaca tcacgaacct cggcgccatc ctcttcgccc gcgatctctc
     3001 gaagttttca gctctgagcc ggaaacaagt tcgcctggtt gtttacaagg gtcgtgatcg
     3061 ctcggagacc attcgggagc aaggaggaca gcgcggttac gcaagcggat tcgcgggcct
     3121 tgtcgcctac ctcaatgatc tgctgccggc aaatgagcag atcatgcagg catttcgaac
     3181 tgaggtcagg atgtaccctg agatagcgat tcgtgagttg atcgcgaatg cgctgattca
     3241 tcaggacttc agaatctcag ggacaggccc aatcatcgaa gtgttcgctg aacggattga
     3301 gatcaccaat ccaggcaaac ctctgattga cacggaacgg ttcctggacg ctccaccgca
     3361 gtcccgaaac gaaaagctcg gagctctcat gcgacgcatg aaaatctgcg aagagcgagg
     3421 tagtggaatc gacaaggtaa tcaaagccgt ggagatgttc cagttgccag cgccgcggtt
     3481 cgacgttacg gatcagcaca caaaagtaac tctttacgct ccgcaaaagc tctcatcgat
     3541 ggagagggac gatcgtgtac gagcctgttt ccagcatgcc tgcttgcgtt acgtttccaa
     3601 cgatacgatg acgaacactt ccttgcgcga gcggttcggc atcgctgccg aaaactactc
     3661 cattgcgtct cggatcatcg ccgagacgat cgaagcgggg atgatcaaac gtagtgatcc
     3721 tgaaagtaag tcaaacaaac acgcaaaata cgtcccctac tgggcctaga tttgtgtgat
     3781 cagaagtggc gccaatggga gactgaagtt tactcatgta ttgtaaatga ctgaattagc
     3841 gagacttgct gctacaactc ttatgtgacg gctatgtgat cggcagcgct cgccagctag
     3901 gggatgcttg caaatggaat gtccaactga aagcgtcacc ctcgcgtcca cggcacatcg
     3961 ggaacaccgt acaggtggtt cgcgtacctc gccgcgacga acaggtaatc cgatagccgg
     4021 ttgaggtagg tgatcgacgc gactgtcgcg gcatcgtcat cgcgtagcgc gaccacgagc
     4081 cgttccgcgc ggcggcagat cgtacgcgcg acgtgaagat gcgctgccgc ggtgctgccg
     4141 ccggggagga tgaacgtctt gagcggcgcg agctcggact ccatcgcgtc gatcgactgc
     4201 tcgagcgccg cgatccgcgc ggggtcaggg ccgtcgaatc gcgacaggcc gggcgaagcc
     4261 agatgggcgc cgatatcgaa aaggtccgac tggatcgcgt gaagctgcgc atcaccggaa
     4321 gatgatggcc aggttgcgcg ggccacgccg atgaacgagt tcaactcgtc gaccgtgccg
     4381 taagcctcga tgcgcgcgtc gtctttgcgg acgcgggcac cgccgaacag cgacgtctcg
     4441 ccgctgtcgc cggttttcgt atagatcttc attggggtct tgcctgatgc ctcgcgacga
     4501 agtaaccccg caaccgcgaa ctacccctcc gcttcgagct ggcggatccg ttccttcacc
     4561 accatctcgg cgaggtcggg aatcacttcc caagcaatgt cgcggacgac cttgtcggcc
     4621 atgagctgca caacacgacg ggcaatccga tcaacctgag cgtcagtcag atcgggggtc
     4681 aggtcggccg gcacggcgcc agcgtccgtg acggagtcgg tgatctgcga attgacctcg
     4741 gtgctcgcag cagccgcttc aggggccgcc ggctcagtcc atggcgcttc ttcggccagg
     4801 gccggctccg gcacctctgc cgcgaacggc gattcagctt gcgaggtatc cgcaaacgga
     4861 tccgcggtcg gcaattcttc gggagcggac catgcgggtt ctggttccgg ttgcggttcc
     4921 ggctccggtg tcgcggccgg cttggcggcc atcttttcca gctcctcgaa gctcatgcgg
     4981 ggaaatgcgc gcgtctcgcc gccaaatgac gaaggtgcct catcccaggg cgacggctcg
     5041 gccggtgctg ggacttccgc ctgcgtttca aacggcgcgg gtgttgcctg cgcctcggcc
     5101 atctgacgca tctcttcgaa actcatggct gggaaggcgc gcgtagcacc ggcgtcttct
     5161 ttcggctcgt cccacggcga ggtctcctcg gcgggtgaag acggtgctgc ctcgaatggc
     5221 gacggcgttt ccgctggcgt ctcggcagcc ggctgcgatg ccgacgccat ctgctccatc
     5281 tcctcgaaac tcatgcgcgg gaaagcgcgg gtttcgcctc cgaacgccgg cgccggttcg
     5341 tcccacggtg agggctcggg agcgggtgcc tcctcctgat gcggggccgg tgtcgaagga
     5401 gcgttcgcca tctgctgcat ctcttcaaaa ctcatccgcg ggagcgcccg cgtttcgttt
     5461 ccgtatgccg gttccggtgg agcgtcgaaa ggcatgtccg tggtcgtcgg cgacgctgcg
     5521 gccggtgcgg cggagaagat gtcgtcgtcg tgtgcaaatg gatcgactgc cgctgcgggc
     5581 gccggctcct cgaacgaggg catgtcggcg aaaagtgcgg gagcgacagc ctcttccggt
     5641 tcggctgcgg acgcattcga ccggctttgc tgcacgagct cttcgacttt gtggatgagg
     5701 ctttgcgatt cgaacggctt cgtgaccacg gcatcgcagc cggctttgtc ggcgcgatcc
     5761 ggatcgaacg gctcaaacgt tccggtcagc aggatcacgg ggatgttgcg atactcggga
     5821 tgcgacttta cgtactcgca cacctcgtag ccattcttct ccggcatgat gatgtccgac
     5881 aggatgatgt ccggacgcat ctccgatagt ttctggatcg ctttcgcgcc gttgttgacc
     5941 gccgtgactt cgtaatcacc gtctgaaaac gtcagttcga cgactttctg gatggtgatg
     6001 ctgtcatccg cgaggaggat tttctttgcc atggctctca ctccgattga ttacgaagaa
     6061 ctgtagcaga agaacttcga tgacggggac gagagctgcc ggcggcggac ggaactaggc
     6121 gtgcggttgc tgtttctcac gcttgaagac cataccgatg tgatcggtcg cgccggtctt
     6181 gaaattgatc gatgtgatgc tgaccaactc ccatccatcg tcgcccaggc gattcaactc
     6241 ttcggcgcgc cgcggatcca tcgagtaatt ctccgaccgg acgttgatga tcttgtactc
     6301 ccaggtcttc atggtccgcc ccctcccgcg gtgctgattg ctgtgcggaa agtgtagcag
     6361 ggttggggcg cgggttgtcg ggagtcgctg gtcgggggtc gggagtcgga gccggcaaca
     6421 cccatcgcga ttgcccgacg agtgatacca cccgttcgga agtggcagtc atcatcatca
     6481 gaagtcgagc aattacgccg gcgccggacg cccgacacgc gagcccgcct cgtttgatat
     6541 cgtcgcgcat cgcgattcgg agattcttct ccgactcccg acacccacac caatgcccac
     6601 ccgtcccttt ctcatcctca tcgtgatcga cggcttcggc tgccgccgcg agtcggagta
     6661 caacgccatt gcccaggcgg atacgccgcg cttcgatcgg ctgtatcggg agtcggcgtg
     6721 gaccacgctc gaagcctctg gccgcgctgt cggattgcca cgcgatcaga tgggtaattc
     6781 cgaggtggga catctcaaca tcggagcggg gcggatcgtc gatcaggaca tcgtccgcat
     6841 cggcaaggcg gtcgcgcggc acgagctgga agccaatccg acgttgatcg atctcgtgcg
     6901 gggcggagga gcggttcatc tcgtcgggct cctgtccgac ggtggcgtcc attcgatgca
     6961 gacgcacttg cacggactca tcgacgccgc aatcgcactc ggcgcgaaag aagtcttcgt
     7021 tcacgccatt ctcgacggcc gcgacacgcc gccgaagtcc gcggagaaat acctggaacg
     7081 gctcgcggag cacatcaagg acaagcccga ggcgcgcatc gctacgatca tcggacgcta
     7141 cttcacgatg gaccgcgaca agcgttggga tcgcgttcag cgcggctacg acctcatgac
     7201 cctcggcgtc ggcaccgaaa caaaagaccc gatcgagacg gtacgtcgct tctacgaaca
     7261 gaacgtgacc gacgagttca tcgagccgat atcggtgctc acacccgaag gcgcgcacaa
     7321 agggcggatg gaagacggcg acgcggtcat cttcttcaac ttccgcgcgg accgcatgcg
     7381 ccagatcgtc actggtttta aggacgctga cttcgacggc tttcaccgcg ccgtaaatcc
     7441 gaagctgcgg atcgccacga tgaactccta tcgcgaagag tggacgctgc cggtgctctt
     7501 ccctccgcag gaggtgaaga accacctcgg cgaagtcctt tcccacgcgg gcctgaaaca
     7561 gctccgcatc gccgagacag agaagtacgc acacgtcact ttcttcttca acggcggctc
     7621 cgatacgccg tcgccgggcg aagaacggat tctcgtgcct tctccgaaag tcgccacgta
     7681 cgatctgaag ccggagatgt cgatcgtcgc gctggcggac aaagtcgtcg aggcggtggc
     7741 ctcgaagaag ttcgacgtca tcatcatgaa tatcgccaat cccgatatgg tcggccacac
     7801 cggcgtcatg agcgcggcga tcgaagcggt gcatgacacg gacgtcgcgg tcgatcgcat
     7861 cctcgacgca gtcgagaaga tcgatggcat tgcgctgatc accgccgacc acggcaacgc
     7921 cgaattcatg ttcgactcgg cgaccggtca gccgcacacg gcgcatacga cgaaccctgt
     7981 tcctctgatc ctcgtcgatt ctcacaatcg gttcggcaaa cttcgaagcg gcggctcact
     8041 gcagaacgtc gcgccgacca tgcttgccat cctcggcatc gcgcagccga aggagatgac
     8101 gggcgagtcg ctgttggaag gatgaataat ccgacgggtt tcatccttca cccctcattc
     8161 ttcttccttt gatcggagtg cgccttgatt ctcgagcaga ttactgacca actgacagaa
     8221 actctcacct cgcgcgtcct cacgctcttt ggccacgcca tcgatcgtgt cgtgctgcag
     8281 tcgccgccgc gtctggcgat gggcgatctg gcgacgccgc tctgcctgga gctggccaag
     8341 gcgctgaaac ggaagccgcg tgagctcgcc gaggcgatgg tggacgggct gacgctgccg
     8401 atgttcgtgc agtcggtgaa cgtcgaaggc gccggctatc tgaacttccg gttcgaccgc
     8461 ggcgcgttca ctgcggcaca cattcgcagt gtgatgacac cggcggcgcc aaccggcgag
     8521 cgcgtcatcg tcgagcacac gaacatcaat cccaacaagg cggcccacat cggccacctt
     8581 cgcaacgcaa tcctagggga cacctacgtc cgttgcatga agtggctggg gtatcgcgtg
     8641 gagacgcaga actacatcga tgacacgggc gtgcaggttg cggacgtcgt cgtcgccttc
     8701 cagcacctcg agccgaagtc actcgcggat gttcgcgcga tgatcgacga ccccaacgtc
     8761 cgcttcgact actactgctg ggatgtctac tcgaagatgg ccagctacta cgcggcgcat
     8821 cccgaggcgc agcagtggcg gcgggacacg ctgcacgaca ttgagcggca cgagggcgag
     8881 acgttcgatt tcgcgtcgac gattgcgcgc gcgatcgtca agcgccacat cgcaacgatg
     8941 ctccgcctcg gcatcaccta cgaccttctg ccgaaagaga gcgacatcat cgcgctgcat
     9001 ttctgggacc gcgcattcga gctgctgaag gccagcggcg ccgtgattca ggagacggaa
     9061 ggcaaacaca aagactgctg ggtgatgaaa ctcgacgaag cctccgagtt cgaagggatg
     9121 aacgagccgg acaagatcct cgtgcgctcg aatggaaccg tcacctacac cggcaaagac
     9181 atcgcctatc agctctggaa gttcgggctg ctcgatcgca cgttcggttt ccggaagttc
     9241 tggacctatc ccgatggcca cgtgttgtgg gagacgacga gcgccgacga cggatcgtcc
     9301 gattcgccgc acttcggcgg tgcgcagaga gtcgtcaacg tgatcgacgt ccgtcaggcc
     9361 tatctgcaga aagtggtgaa ggaaggcgtc cgccaactcg gtcacgcgcg ggaggcggag
     9421 aagtcgattc acttctcata cgaaatggtg gcgctaaccc cggccacggc aaaggcgctg
     9481 gacatcgagg tttccgaaga agactcgaag aagccgttca tcgaaatgtc aggccgcaag
     9541 ggcctcggcg tgaaggcgga cgatctgatc gacgtgttgc agaccaaggc cgccgagtcg
     9601 gtgcgcgaag gacgcggcga agggttgacc gatgcggaga tcgagcaact cggacatcag
     9661 atcgccgtcg ccgcgctccg ctacttcatg ctcaagtacg gccgcaacaa agtcatcgcc
     9721 ttcgatttca acgaggcgct cacgttcgag ggcgattcgg ggccgtacct gcagtactca
     9781 gtcgttcgcg tagagaacat cttccgcaag atggcggagc gggatgtcga tgccagactg
     9841 gatgatgcgt cactcgacgc acttacgctg accgagggac tcacggacga gatgtgggaa
     9901 ctggtgcgat tgagcgctga gctgccggcc gcgatccgca gagccaccga cgcgctggag
     9961 ctgtcgatca tcacccacga cctcttcgag ctggcccaga agttcaacag tttctaccac
    10021 aagtatccga tcctcaacga gaaggacgcc gacgagcggc agcgccgcgc cgtgtgcgcc
    10081 gaggtgttcc ggcgcacgat ggcgggagcg ttggaattgc tggggattcc ggtgccgcag
    10141 agaatgtgat tattgcgcgc ccctggcggg cgcgcgttgt cggttgtcgg ttgtcggtgg
    10201 agtgaacgac gtttctttgc ggtgaactca ggctgcgaag gggatgatct ccagctcagc
    10261 gccggagtcg acatgggcaa ggatctcctc cactcttcgg aacgtcggat cgtcgatcga
    10321 gtactcgtgg cactgcgggc actgaagcac cggaagagac ttcaggatca cgatggaccg
    10381 ttctccggtc ttgaagggga gatcagtatt cgtgggttgc tgcgtccctc cgcaaactcg
    10441 gcatttcatt tggatctcct catcgtcaga ccgggatgcc actctacctc ggggtccagt
    10501 cagtcggatt ctctgttaca ataattctca cggcgctcca atcgccgcaa ccctcatgaa
    10561 actcgatctt caggacatcg cgttgcggac gctcatcctt gttgccggtg ggctggctgc
    10621 gttggtgctg tcgctcaagg ggcaggggca gacccttccc gcgctcgccg tgggcggcac
    10681 cctcggagcc ttcatgatgg cgcgtttcgg gccgacacag cactaggccg ctggcctagg
    10741 aggcgttccg ctcgccttcc gcccggtgga cgttctcggc ctgcaatccc ttgggaccct
    10801 gcttgacgtc gaactcgacg cgctcgccct cgttcagcgt ccgatacccc tgagcctgga
    10861 tggcgctgtg atgaacgaac acgtcttccc cggtgtcacg tttgatgaaa ccgaagcctt
    10921 tgtcgttgtt gaaccactta acggtaccga tctccgccat ttcaacctcc agcctggcaa
    10981 cgcgatcctt tcgccgcgtc aagccggctg gacgtcaaat tgtgcaatgt tcaggcccct
    11041 ttttggccgc cattcaccag atcagggcgc ggttcatgcc gatccgctgg tagccgaggg
    11101 actcgtagag ggcgatggcg ggaaggttga agtcgttcac gaatagcgtg acagtcttcc
    11161 ctttccgcgc gagatggccg cacacctcgc gaagaagggc gcgggcatgg ccgtgccggc
    11221 gaaggcgcgg gttcgtccag actcccatga gctgcaccat gtcgtcggtg accgccgagt
    11281 actcgcattt cgcggcgatc atcccgtcga cgatccggat taccgagcgc ttctgttcga
    11341 ccagctcgcg gacgcgctcc cgatagccgg ccgagtcgcg ttccagcgga ttgatcccca
    11401 cctcctcgat gtgcatcgac gcgcacgcgg ggacgagcgc ctcgagatcg cgcgtggtcg
    11461 cgtaacgcgc ttcgctcaga tcgggaaagt cgagcctgcc tcggatcgca tagaccggct
    11521 gattcatccg gacgaccgtc ggcggatcga ggcggttgtg cagtcgtgcc cagagctgct
    11581 ccacgagatg agccggcgag atgatggcgc gtaccggcag catcttcgtc agaatccggt
    11641 ccgcgatggc ggcgatggcg gtctccgtga ggtctggcga taacgcgcgg tcgccggcca
    11701 gaacgatatt cgtggccagg gacgcgacca ggacgatctc gcggttatgg cgcacttcga
    11761 ccgtttgtgt cgacgacgca aagcgctctt cgttgatgcg tgcgatcaga tacacgttga
    11821 tcagcggatc gcgccggaga aaggccaggg tctccgcgat gttgtcgcga ccgacagact
    11881 tgatcgacca gcgtccttcg acgggaagag tggccatggg atgaacaata gattagtgga
    11941 tttggatggt aggggatgag agatcagtgg atcagagaca agagaccgga gccaaacgga
    12001 tcgggtatct caaaactaat tgcccggtcc taaaccaccg ccatccacta aaactgcgag
    12061 ctcgccagat acgccagacc
//
LOCUS       DATLVW010000720         4010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_29555, whole genome shotgun sequence.
ACCESSION   DATLVW010000720 DATLVW010000000
VERSION     DATLVW010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29555"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="VNN25_21010"
     CDS             complement(<1..1805)
                     /locus_tag="VNN25_21010"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH94069.1"
                     /translation="MTGHLFESTLVLAVAILLAQLPRLAARTRYAIVFAALMKFAVPS
                     AIVPRVLAFLGIELAHVPRGTIVIEALGPLSAADRSEPAVSLWPAMLITLWLVVAIGL
                     LARALLRGRIALRRALAEASDATPGELAALGRAISRARLERPVRLVRSESISAPATAG
                     MLRPVILIPAATHLAADELETILTHECAHVARRDNLLGLVESIAGCALWFHPLVWIAR
                     RILDGTREEACDAVVIASSDSGTYINALRKVCEAAIGPRVAAVSCIVSNTIHERMEAI
                     MRFGSRRLLNHRAVIAVVLALLTATTLGIGVARALPSGGSPDSKYKVDVTLTRDVSSD
                     VFNFAVTVSDRASGVVVSTANLRSRVEQWSTATSEMTSKKSGHHTAVVRAIGHADGTA
                     EVETTVDQEPAIVARIAAKSEAARSSNAVGVSGDSPLRVGGDVKPPVIINRVEPIYPA
                     LAKASRISGVVILEVVIDHTGVVKDARIVKPLPFGLDQAALDAVKQWTFRPATLNGTA
                     VDVLFNLTINFRAAEPAAPRTIAQGPTPSGGEPISIDLKDADIRDVMKTFSLATNMTI
                     LVDDDIRGPVTVSVRDTPWMDAFDHILRDANLRYER"
     gene            complement(1802..2185)
                     /locus_tag="VNN25_21015"
     CDS             complement(1802..2185)
                     /locus_tag="VNN25_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXH94070.1"
                     /translation="MSKERIALTKFETEVMSVLWRLGEASVRELQEAIDRADRPAYTT
                     VQTIVQRLEQKGAVRRTRKAGNAYFFEPAVTRKSVYRRLVEELLDLVGGSQPLVAHLV
                     ETGKLSLDDLKAIESSAKTPKRGRK"
     gene            complement(2274..2786)
                     /locus_tag="VNN25_21020"
     CDS             complement(2274..2786)
                     /locus_tag="VNN25_21020"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HXH94071.1"
                     /translation="MKIRILTLGLCLITVAAFGQANPKLKELNYFDGTWQCSGTSFAS
                     PMGPEHPTKATVTAAWILGGTWLEAHYKEVKTAKNPTPFELRAFWGYDEQPKSLVAGT
                     VDNMGGYSTATAAWDGDKLTFTGPMHGGGMSSNGRDVFTKVSKSEFRHEGEMEDKGKW
                     IKLDEETCKR"
     gene            complement(2850..3341)
                     /locus_tag="VNN25_21025"
     CDS             complement(2850..3341)
                     /locus_tag="VNN25_21025"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXH94072.1"
                     /translation="MNELDPAVQREIDALGIPHEVLACNPEWADTDTFCANYGIPREN
                     AANTILVALKTEPRSYVACLVTAATKLDVNHKVSKLTGVRRMSFASAEETAALTGQMI
                     GGVTVFGLPEGVPLYVDTEVMRREYIVVGGGNRSTKIKLAPEHLRKMARTIVSEIAVP
                     RPA"
BASE COUNT          733 a         1275 c         1265 g          737 t
ORIGIN      
        1 cgctcgtagc ggagattggc atcgcggaga atgtggtcga aggcatccat ccacggcgtg
       61 tcacgcaccg ataccgtaac cggccctctg atgtcgtcgt cgacaaggat ggtcatgttc
      121 gttgccagcg agaacgtctt catgacgtcg cggatgtcgg catctttcag atcgatcgaa
      181 atgggttcgc cgccactggg tgtgggtccc tgcgcgatcg tgcgcggggc tgcgggctcg
      241 gccgctcgga agttgatcgt gagattgaag agcacgtcca cggccgttcc attcagagtg
      301 gcgggacgga acgtccactg cttcaccgca tcgagcgcag cctgatccag gccaaatggc
      361 aatggtttga cgatgcgcgc atccttcacg acgcccgtgt gatcgatcac cacttcgagg
      421 atgacgacgc cgctgatcct cgatgccttc gcaagcgctg gataaatcgg ctcgacgcga
      481 ttgatgatta ccggcggctt gacgtcgccg ccaacgcgga gcggcgaatc gccggagacg
      541 cccactgcgt tggaactgcg ggctgcctca ctctttgcgg cgatcctcgc gacgatcgcc
      601 ggctcctgat ctacggtcgt ttcaacctcg gcggtgccat ccgcatgacc gatcgcgcgg
      661 acgacggccg tgtggtgtcc ggacttcttt gacgtcattt cagacgtcgc cgtcgaccat
      721 tgctcgacgc ggctccgaag gttggccgtt gagacaacga cgccgctggc tcgatcgctc
      781 acggtaacgg cgaagttgaa cacgtcggac gacacgtcgc gggtcagcgt cacatcgacc
      841 ttgtacttcg aatccggact gcctcccgag ggaagagcgc gggctacgcc gatccccagc
      901 gtggttgccg tgagaagagc gaggacgacc gcgatgacgg cgcggtggtt gagaagacgg
      961 cgtgatccaa atcgcatgat ggcctccatt cgttcgtgga tggtgttgct gacgatgcac
     1021 gagactgcgg ctacacgggg tccgatggcg gcctcgcaga ctttgcgcaa tgcgttgatg
     1081 tacgtgccgg aatcgctcga tgcgatgacg accgcgtcgc aggcttcttc acgcgtgccg
     1141 tcgaggatgc ggcgtgcaat ccagaccagc gggtggaacc agagcgcgca gccggcgatc
     1201 gactcgacca gaccgagaag gttgtcgcgc cgcgcgacgt gagcgcattc gtgcgtcagg
     1261 atcgtctcca gctcgtccgc cgcaagatgc gtcgcggccg ggatgaggat gacgggacgt
     1321 agcatgccgg cggtggcagg agctgaaatc gattccgagc ggacgaggcg aacgggtcgt
     1381 tcgaggcgcg cccgtgaaat cgcgcgcccg agggcggcca gctcgccggg cgtggcgtcg
     1441 gaagcttcgg cgagagcacg ccgcagtgca attcgtccgc gaaggagagc gcgggccagg
     1501 aggccgattg caaccaccag ccagagtgtg atcaacatcg ccggccatag cgaaacagcg
     1561 ggttcgctcc gatcggcagc gctcagcggt ccgagcgctt cgatgacgat cgtccccctc
     1621 ggcacgtgcg ccagctcgat gccgaggaat gcaagaacgc gcggaacgat cgccgagggg
     1681 accgcgaact tcatcagtgc cgcgaaaacg attgcgtaac gcgttcgcgc ggccaggcgc
     1741 ggaagctgcg ccaggagaat agcaaccgcc agaacgagcg tcgactcgaa aagatggccg
     1801 gtcatttccg gcctcgcttc ggcgtcttgg cgctgctctc gatcgccttc aggtcgtcga
     1861 gcgacagttt gccggtctcg acaagatgcg cgacgagcgg ctgcgagccg ccaaccaggt
     1921 cgagcaactc ttcgaccagc cggcggtaga ccgacttgcg tgtcacggcc ggctcgaaga
     1981 agtatgcgtt gccggctttg cgcgtgcggc gcaccgcgcc tttctgctcc aggcgctgca
     2041 cgatcgtctg cacggtcgtg taagcgggac ggtcggcgcg gtcgatggct tcctgaagct
     2101 cgcggaccga tgcctcgccg agccgccaga gcacgctcat gacttcggtc tcgaacttcg
     2161 ttagcgcgat gcgttctttg ctcatcgcgc ggcatactac aactgtagta gcaacgagtc
     2221 aagaaaaaag ggaccggcag agcgccggcc cctcgtcgcg tgcgcaaact caatcagcgc
     2281 ttacaggttt cctcgtcgag cttgatccac ttgcccttgt cttccatctc gccttcgtgg
     2341 cggaactcgc tcttcgagac cttcgtgaac acgtcccggc cattcgacga cataccgccg
     2401 ccgtgcatcg gcccggtgaa cgtgagcttg tcgccgtccc aggcagccgt cgccgtcgaa
     2461 tagccgccca tgttgtcgac ggtgccggcg acaagcgact tcggctgctc gtcgtagccc
     2521 cagaacgcgc gcagttcgaa gggagtcgga ttcttggccg tcttcacctc tttgtagtgc
     2581 gcttcgagcc aggtgccccc cagtatccag gcggcggtga cggtggcctt ggtgggatgc
     2641 tccggtccca tcggcgacgc gaacgatgtt cccgagcact gccacgtgcc gtcgaaatag
     2701 ttcagttcct tcagcttcgg attggcctgg ccgaacgcgg ccaccgtgat gagacagaga
     2761 cccagggtca gaatgcggat tttcatgcga cctcctcctt ttggtaacac ttacgccgtt
     2821 cgtggcgctt tggatgccta atctatagtt taggctgggc gcggcacggc aatctcggac
     2881 acgatcgtcc gagccatttt gcgaagatgc tcgggcgcca acttgatctt ggttgaccgg
     2941 ttgccgccgc cgacgacgat gtactcccgc ctcatcacct cggtatccac atacagcggc
     3001 acgccttccg gcaaaccgaa aacggtgacg cctccgatca tttgccccgt cagcgccgca
     3061 gtctcctcgg cggacgcaaa cgacatgcgc cggacgccgg tcagtttgct caccttgtga
     3121 ttgacgtcca gcttcgtcgc cgccgtcacc agacacgcga catagcttcg cggctccgtc
     3181 ttcagcgcga cgagaatcgt gttcgccgcg ttctcccgcg ggatgccgta gttcgcgcag
     3241 aaggtatcgg tgtcggccca ttccggatta caggccagaa cctcgtgcgg gatgccgagc
     3301 gcatcaatct cgcgctgcac cgccggatca agctcgttca tgaacacggg attctatctg
     3361 gagtgcggtc gtctcgaccg cgggccctcg ggcgtctcgc ccgagggctg tcagggcgga
     3421 ggtcgaaatt cggagacgtg cgtcccctat gccgttgtcc acgatccaga agacgacgcg
     3481 cggtggatcg tgcggcgtgg cgcgacgccc tcgacgcgaa tgttctcgta gcgggctgac
     3541 ggcgacgcca cccaagtcca gtgcgacacc tgccgcggcg aactgcgaca gccccgtggc
     3601 gcaacacgac atccaccgcg acgcagaaca ccgacctagt cccactggga caagcaccgt
     3661 gtaccactgg gacaccggcc gtgtaccact gggacaccga ccaagtacca ctgggacacc
     3721 gcccgtgtac cgctgggaca ccgaccatgt accactggga caccgaccgt gtaccactgg
     3781 gacaccgacc gtgtaccact gggacatcga ccatgtacca ctgggacatc gaccatgtac
     3841 ctgtgggaca ccgaccgcgt accactggga catcgaccat gtaccagtgg gacacccacc
     3901 atgtaccggg acacgcactg tgtcccagtg gggtacgcac tgtgtcccag tggggtacgc
     3961 actgtgtccc agtggggtac gcactgtgtc ccagtggggt acgcactgtg
//
LOCUS       DATLVW010000721         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_483539, whole genome shotgun sequence.
ACCESSION   DATLVW010000721 DATLVW010000000
VERSION     DATLVW010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483539"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VNN25_21030"
     CDS             <1..425
                     /locus_tag="VNN25_21030"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXH94073.1"
                     /translation="GQVADLIATGKNEATAEQLDFIHRNKTGKLLTASVVLGGLLANA
                     DDEKIEALRNYGRAIGLAFQIVDDLLDQEEDATTLGKTAGKDLAQGKLTYPALFGTDA
                     ARAAIDTLLSEAIRNAAIIGGPVNYLAGIARFICERRS"
     gene            439..>2248
                     /gene="dxs"
                     /locus_tag="VNN25_21035"
     CDS             439..>2248
                     /gene="dxs"
                     /locus_tag="VNN25_21035"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXH94074.1"
                     /translation="MTLLETINSPQDLRSLNRRQLHELAGEIRHRIIEVVSKIGGHFG
                     GNLGIVELTLALHYTFDTPRDQIVFDTGHQTYPHKLITGRRDTFETIRMHGGISGFCK
                     REESEYDVFNAGHASTSISAALGIAVARDIKADNYRVVAVIGDGALSGGLALEGLNQA
                     GHLKRKLMIILNDNDMSISTNVGAMSGYLNGIIKGQTYNQAKDLARGIMDRIPLVGGK
                     LHGMASDMEQLFKHMIVPGTLFEELGFKYLGPYDGHDLDFLIDLFEKHKDYNGPLLVH
                     VITKKGKGYVPAENKPIWSHGVTPFDIGSGEVVKSKPAPPSYTAVFAETLIELAKRDP
                     KIVAITAAMPDGTGLDKFAKAHPDRTFDVGIAEEHAVTFCGGMATQGMKPIAAIYSTF
                     LQRAFDQVFHDVAIMDLPVVFALDRGGIAGADGPTHHGIYDMAYLRIFPNMICMAPKD
                     ENELRHMLKTSFETGHPTSLRYPRGNGFGVTMDAELQSLPIGKGEVLREGSAGAIFAI
                     GNEVWPSMQAAEILAKEGIEVAVINARFIKPLDDELITKYCQPFARIITVEEGSLAGG
                     FGSAVMERVQKLGIRGVAFHRIGIPDEYVHHGAQDVL"
BASE COUNT          473 a          717 c          673 g          385 t
ORIGIN      
        1 gcggtcaggt cgccgatctc atcgccacgg ggaagaacga ggccactgct gaacagctcg
       61 acttcatcca tcgcaacaag acgggcaagc tgttgacggc ctccgttgtc ctcggcggac
      121 tactcgcgaa cgcggatgac gagaagatcg aggcgcttcg aaactacggc cgcgccatcg
      181 gtctggcctt tcaaatcgtc gacgacctcc tggaccagga agaggatgca accacactcg
      241 gaaagacggc aggcaaggat ctggctcagg gaaaactgac ctacccggcg ctcttcggca
      301 cggatgccgc acgcgccgcc atcgacacgc tcctgagcga ggcaatccgc aacgctgcta
      361 tcatcggcgg tccggtgaac tatctcgccg ggatcgcgcg tttcatctgc gaacggcgat
      421 cgtgaggcgc cgcagccaat gacgctcctc gaaaccatca attcgccgca agacctgcgg
      481 tcactcaacc gccggcagct ccacgagctc gcaggcgaga tccgtcatcg catcatcgag
      541 gtcgtctcga agatcggcgg tcacttcggc ggcaacctcg gcatcgtcga gttgacgctg
      601 gctttgcact acacattcga tacgccgcgg gatcagatcg tcttcgacac cggccatcaa
      661 acctacccgc acaaactgat cactggccgc cgcgatacgt tcgagaccat ccgcatgcac
      721 ggcggtatct ccggattctg caagcgggag gagtcggagt acgacgtctt caatgccgga
      781 catgcctcga cgtcgatctc ggccgccctc ggcatcgccg tcgcgcgcga catcaaagcc
      841 gacaactacc gcgtcgtggc ggtcatcggc gacggcgcgc tctccggcgg cctggcactc
      901 gaaggtctca atcaggctgg tcatctcaag cgcaagctga tgatcatcct caacgacaac
      961 gacatgtcga tctccaccaa cgttggagcg atgtcgggtt acctgaacgg gatcatcaaa
     1021 ggccagacct acaaccaggc gaaggacctg gccagaggga tcatggaccg catcccgctc
     1081 gtcggcggca agctccacgg catggccagc gacatggagc agctcttcaa gcacatgatc
     1141 gtgccgggta cgctcttcga ggagctcggc ttcaagtatc tcgggcccta cgacgggcac
     1201 gatctcgact tcctgatcga tctcttcgag aaacacaagg actacaacgg tccgttgctc
     1261 gttcatgtga tcacgaagaa gggcaaaggt tatgtgccgg cggagaacaa gccgatctgg
     1321 tcgcatggtg tgacgccgtt cgacatcgga tcaggcgaag tcgtgaaatc gaagccggcg
     1381 ccgccgagct acacggcggt cttcgccgag acgttgatcg agctcgccaa gcgcgacccg
     1441 aagatcgtcg ccatcaccgc ggccatgccg gacggcacgg ggctcgacaa gttcgcaaaa
     1501 gctcatccgg accgcacgtt cgacgtcggc atcgccgaag agcacgcggt cacgttctgc
     1561 ggagggatgg ccacgcaggg catgaagccg atcgctgcga tctactcgac cttcctccag
     1621 cgagcctttg atcaggtgtt ccacgacgtg gccatcatgg atctgccggt cgtcttcgca
     1681 ctcgatcgtg gcggcatcgc gggcgccgac ggtccgacgc accacggcat ctacgacatg
     1741 gcctacctgc ggatcttccc gaacatgatt tgcatggctc cgaaagacga gaacgagctc
     1801 cggcacatgc tgaagacgtc gttcgagaca gggcatccga cgtcgctgcg ctacccgcgc
     1861 ggcaacggat tcggcgtaac gatggacgcg gagctgcagt cgctgccgat cggcaaaggc
     1921 gaagtcctgc gcgagggaag tgccggagcg atctttgcca tcggcaacga agtgtggccg
     1981 tcgatgcagg ccgcggaaat cctcgcgaaa gaggggatcg aagtcgcggt catcaacgcg
     2041 cgcttcatca agccgctcga cgatgaactg atcacgaagt actgccaacc gttcgcacgc
     2101 atcatcacgg tggaagaggg ctcgctcgcc ggcggcttcg gttccgcggt gatggaacgt
     2161 gtgcagaagc tcggaatccg cggcgtcgcc ttccaccgca tcggcattcc cgatgagtat
     2221 gtccaccacg gcgcgcagga tgtgcttc
//
LOCUS       DATLVW010000722         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_239898, whole genome shotgun sequence.
ACCESSION   DATLVW010000722 DATLVW010000000
VERSION     DATLVW010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239898"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VNN25_21040"
     CDS             <1..959
                     /locus_tag="VNN25_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94075.1"
                     /translation="TDDATGTYARYAYGFQDFNDYPKVGVWADGYYITFNMFDRNENS
                     IGGKICALPKDKLLAGTDAPMQCFDVPEVGLLPADIDGLTPPPADSPEIVMNFTRDAL
                     QMWKVHIDWSDATKSTLTGPVKIPVASFDPACGGGACIKQKGSTGRLDTLSDRLMFRL
                     AYRNFGDHDALVVNHSVSVAGSAVSGLRWYEIRNPLGTPTVTQQSTYAPGTSARWMGS
                     VAMDKMGNMLAGYSASGSSLFPSIKIAGRAADDPKNKLSKELTVIAGSAAQADSDRWG
                     DYANMTVDPTDDCTFWFTTEILDKNHELWQTAFMHTKFSNCN"
     gene            complement(1045..2916)
                     /locus_tag="VNN25_21045"
     CDS             complement(1045..2916)
                     /locus_tag="VNN25_21045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH94076.1"
                     /translation="MIRAALLAISIVFLIVPAMHAAEYTQDNACASCHAAKFASYQGV
                     GMAQSMRRPRAEVLIEDFRNAKFFHAPSQNYFEMAWTDGKLFFKRYQLDGAGQRINEI
                     EQRVDWIVGSGHRSRVYFYRVPSGEMYQLPIAWYTQEKKWGMAPGYDRADNDGITRQV
                     RRECLFCHNAYPAEVKEGSDTHWKAQIFPGELPEGTGCQRCHGPGARHVDVASRGGSV
                     ESVRAAIVNPVRLAPALRDSVCFQCHLLPSVSLIGVRRFERGDYSFRPGQLLSDYILH
                     VDIEEATRPRDQRFEINHHAYRLRQSLCYIKGGITCINCHDPHQPLAKDPRLARVTTI
                     CLGCHARHEPKAQDVAADDCVRCHMPRRRTQDVVRVVMTDHRIQRRPPPGDLLAPIPE
                     HDPDIRSVSFYDRDAALAGTLGDAYRAVTLLRAMPRNTDAAEQLTKNLGATTSPVPRF
                     DLIAAQLQQRQFQSALETLATLDHTAPSDMLLRDWRGIAEVGLGKTDDGIADMRAAAA
                     ASPDQPEYQFNLATVLHRSGRDEEALPLLTRSVTLRPNFVAAWIMRAEALSALKRPSE
                     AINDLQRALAIDPRQTKAYLLLARLLTESGKQSEAQRWLRHGARVAAKPGEIEAALR"
     gene            complement(3364..>3787)
                     /locus_tag="VNN25_21050"
     CDS             complement(3364..>3787)
                     /locus_tag="VNN25_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94077.1"
                     /translation="RLVGNFSNEKELFFMMGKFSPQLKELPGTVDMSTPATIEGRVPA
                     AWIPPGSQPGMTPTPAHREENGDRLRVAQSLLEREIARTDLAPAEAEALREVLYALRE
                     RNVTRVGGIYRGAFAELKTQSEGVRQAFEQLDGYFGTR"
BASE COUNT          732 a         1191 c         1200 g          664 t
ORIGIN      
        1 ccacggacga tgccaccgga acgtacgcgc gctacgcata cggttttcag gacttcaacg
       61 actacccgaa ggtgggcgtg tgggccgacg gctactacat cacgttcaat atgttcgacc
      121 ggaacgaaaa ctcgatcggc ggaaagatct gcgcgctgcc gaaagacaag ttgctcgcgg
      181 gcaccgacgc accgatgcaa tgcttcgatg tccccgaggt cggactgctg cccgcggaca
      241 tcgatgggtt gactcctccg cctgccgact cgcccgagat cgtgatgaac ttcacccgcg
      301 atgcattgca gatgtggaag gttcacatcg actggagcga tgcgacgaaa tcaactctga
      361 ccgggccggt gaagatccct gttgcctcgt tcgatccggc ctgcggcggc ggcgcgtgta
      421 tcaaacagaa ggggtcgacc gggcgtctcg acacgctctc cgaccggctg atgttccgcc
      481 tggcgtaccg caacttcggc gatcacgacg cgctcgtcgt caaccactcc gtgagcgtgg
      541 ccggaagcgc ggtcagcggt ctgcgctggt acgagattcg gaacccgctc gggactccga
      601 cagtcacgca gcagtcgacg tacgctcccg gaacctcggc gcgttggatg ggaagcgtgg
      661 ccatggacaa gatgggcaac atgctggccg gctacagcgc ttcgggctcg tcgctgtttc
      721 cttcgatcaa gatcgccggc cgcgcagcgg acgatccgaa gaacaaactc tcgaaggagt
      781 tgacggtgat tgccggctcg gctgcccagg ccgacagcga tcgatggggc gactatgcca
      841 acatgaccgt cgatcccacc gacgactgca cgttctggtt cacgacggag atcctggaca
      901 agaatcacga gctctggcag accgcgttca tgcatacgaa gttctcaaac tgcaattagg
      961 cgcgggaaac aaatcaacca caaagacaca gaggacacaa agggttctct gtgctctttg
     1021 tgtctcagtg gtgacaagag tctttcagcg cagcgccgct tcgatctcgc ccggtttggc
     1081 cgcgacgcgc gcgccgtgcc gcagccagcg ctgcgcctcg gattgctttc ccgactccgt
     1141 cagcaggcgg gccagcagaa gataggcctt cgtctggcgc gggtcgatcg ccagagcgcg
     1201 ttgaagatcg ttgattgctt cgctcgggcg tttcagcgcg gacagcgcct cggcgcgcat
     1261 gatccaggcg gcgacgaagt tcggacgcag cgtgacggat cgcgtcagca gcgggagggc
     1321 ttcctcatcg cgtccgctcc ggtgaaggac cgtggcgagg ttgaattgat actcgggttg
     1381 gtcgggagac gcggccgcgg cggcacgcat gtcggcgatc ccgtcgtccg tcttgccgag
     1441 gccgacctcg gcgatgccgc gccaatcgcg caggagcatg tcggacggcg ccgtgtggtc
     1501 gagtgttgca agcgtctcaa gcgccgattg gaactgccgc tgctgcaatt gggcggcgat
     1561 cagatcgaac cgcggcactg gtgacgtcgt ggcgccgagg ttcttggtga gttgctccgc
     1621 ggcatcggtg ttgcgcggca tcgcccgcaa caacgtaaca gcacgatagg cgtcgccgag
     1681 cgtgccggcc agtgcggcgt cgcgatcgta aaaggagacg ctgcgaatgt cgggatcgtg
     1741 ctcgggaatc ggcgccagca gatctccggg cggcggacgg cgctgaatcc gatgatcggt
     1801 catcaccacg cgcacgacat cctgcgtcct tcgcctcggc atatgacacc gcacgcaatc
     1861 gtcggcggca acatcctgcg ctttcggctc gtgccgggcg tgacacccga ggcagatggt
     1921 ggtcacgcgg gcgagccgtg ggtccttcgc aagcggctga tgcggatcgt ggcaattgat
     1981 gcaggtgatg ccgcccttga tgtagcagag actctggcgc aatcggtagg cgtgatggtt
     2041 gatctcgaag cgctggtcac ggggacgcgt ggcttcctcg atgtcgacgt ggagtatgta
     2101 atcgctcagg agctggcccg gacggaacga gtagtcgccg cgttcgaaac ggcgcacgcc
     2161 gatcaggctg acggacggca gcagatggca ttgaaagcag accgaatcgc ggagcgccgg
     2221 cgccaggcgc accggattga cgatggccgc gcgaacgctc tccacgctcc caccgcgcga
     2281 cgccacatcg acatgacgcg cgcccgggcc gtggcagcgc tggcatccgg tgccctccgg
     2341 taactccccg ggaaagatct gcgccttcca atgcgtgtca ctcccctcct tcacctcggc
     2401 cggatacgcg ttatggcaga agagacactc gcgccgcacc tgccgcgtga tgccgtcgtt
     2461 gtcggcgcgg tcgtagccgg gcgccatccc ccacttcttt tcctgcgtgt accaagcgat
     2521 gggaagctga tacatctcgc cgctgggcac gcgatagaag taaacgcgcg aacggtgccc
     2581 cgatccgacg atccaatcga cgcgctgctc gatctcgttg atgcgctggc ccgcgccatc
     2641 gagctgatag cgcttgaaga acagcttccc atcggtccac gccatctcga agtagttctg
     2701 cgatggcgca tgaaagaact tcgcgttgcg aaaatcctcg atcaacacct cggcgcgcgg
     2761 acgccgcatc gactgcgcca tccccacgcc ctgatacgac gcgaacttcg cggcatggca
     2821 ggaggcgcaa gcgttgtcct gcgtgtactc cgctgcgtgc atcgccggga cgatgagaaa
     2881 cacgatcgat atcgccagta gcgccgcgcg gatcacaaaa cagatgatag ggcggaacca
     2941 gtcgtcattg tctgcacgga gttacggcga ggaatcagct tcagaaatct gtcgcccgct
     3001 aaagcgggct cgaaaaatgg ggcgactccg tttacccccg gctgaagccg ggggctacgg
     3061 atctcagacc ggattcgtca aatcacgtgg aagcgctgat cgagactgag cagcgtatcg
     3121 ccgctgaagg cccggcgtga gaaccgtagc ccccggcttc agccgggggt aaacggagag
     3181 cgccaagaga ttcccagccc gcttcagcgg gcgacaggtt aggtgggcag acgccatatc
     3241 cagaagaatc ggacgcatgt cggaaccgga tcgagattgc agcactcgtc caaaaacgac
     3301 cgcttcccgc aatcacatcg acaacgcgcg aagcgcaatc aaccgacaac cgacaacgcg
     3361 cagctatctc gttccgaaat acccatccaa ctgctcgaac gcctgccgca cgccctcgct
     3421 ctgcgttttg agctccgcaa atgcgccgcg atagatgccg ccgacgcgcg tcacattccg
     3481 ctcgcgcagc gcgtacagca cctcgcgcag tgcctcggct tcagccggag cgagatcggt
     3541 gcgggcgatc tcccgctcca acagagactg ggcgacgcgc agacgatcgc cgttttcttc
     3601 gcggtgcgct ggcgttggcg tcatgccggg ctgtgagcct ggcggaatcc aggccgcggg
     3661 cacccgccct tcgatcgttg ccggtgtcga catatccacc gtgccgggaa gctctttcag
     3721 ttgcgggctg aacttgccca tcatgaagaa gagctccttc tcgttggaga agttgccgac
     3781 gaggcgc
//
LOCUS       DATLVW010000723         4628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_566347, whole genome shotgun sequence.
ACCESSION   DATLVW010000723 DATLVW010000000
VERSION     DATLVW010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566347"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VNN25_21055"
     CDS             complement(<1..411)
                     /locus_tag="VNN25_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94078.1"
                     /translation="MAEHHLFPRARKRHRVFGGLNARQKHIFNWITVVIVMLYVTGWI
                     GAIHDVIEQRREIARMAKEAEAKVPAVKTPRGKVPVPAPPPPLPATPIATQIFQPQAP
                     TIAYIDDAMLGFLNPLRGKSGKVFFTTLTPGASIV"
     gene            674..3844
                     /locus_tag="VNN25_21060"
     CDS             674..3844
                     /locus_tag="VNN25_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94079.1"
                     /translation="MLDESRKDLAAGVIRSLLWIAAFCFAVGIFLTATLLLRGVPATP
                     HVAVGRVTVENASKLRDYLTVLLFFIIVPVATIPLYRLGARENLRLRAAVSGDGVRNL
                     VSVLFVAPFFLAPFLYLTTFKPAWPILIPLAFSMLLPRAVITWQQTLWIRRLFVREMA
                     PHHALIIVEALSWILFRYIAVGKRIAHIPTLFLEIAFIAFFIALFWLAFLLIARIVAF
                     TSDFTTEEALQRIGIAALPLVILPPLALLFVQGNVAISLAMVMILLALPLTLSRREPI
                     DSRSVRNLVAFAILPSLLFCLSYASTASLTQWIDLFHRGETLGPASDLLRGKVPFRDV
                     FALHGLLEDGLLDTWLMELFGRNVNIVLLRPVVLGSVAVAMLWYLGMVIFDSIPLAML
                     SILLGAVTTVDNERALFEIVAIALLIGALRRNARWLAVASGVAAAITLFYSLDIGLYT
                     VGGGVLAILAASLLRNAAQRSWALLASFVAGIALGAAPFVIYLSMRGAFGAFVETSFF
                     AIPRIIDAIWSLPFPDLTTTFRSNLNLHSLSDFVLSDHFRFILNPLVIGLALVVLVQR
                     RITRRSDALDLGLAVLTAFAILTQRSALGRADFQHQYFSAFLIGPMILILLVLFGRAA
                     AGIWHTHEQATKAFVVLAAAMMLPVFAVILWVPDIINMRVDDTTHYLGRVSRIGWIDP
                     AAEEIRNRVESVKSAVDALSKPGQPMFDFSNQPALYFFCDRPNPTRFYQVPILSPRAY
                     QAETILALERAKPPLVIRHSPQEFDVFDGIDNSIRAQAVAAYIDDHYTFARTTRGVEI
                     WRRRPEATALNLAAYLSRIRIPTVEELGAIGDRSRVVFPSVGSLPGANASYWRSDLTL
                     HNPFKERMPLELRYVSGDSRIDKNVTILGGQSLRSEDVVRSFFGAPEGSGVLWIEYRG
                     QHQPVARIKTYDAAHNARGSVSAPLSMRDSASAGSENQDLTLVGIPNGTDRRVNIGIV
                     NIGKVPAIFRITVRTRSGQKIGKTFEEALNEDELRTIPEIEKTLGVTLDETSALHISM
                     IAGTCVAYASVVDPNGDSQFMPAVAR"
     gene            complement(3876..>4628)
                     /locus_tag="VNN25_21065"
     CDS             complement(3876..>4628)
                     /locus_tag="VNN25_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94080.1"
                     /translation="EGHQGGRARAGQVDAAIAAYRKAVAQEPNNLEARGKLLRAVRFK
                     GAYVASTSDDKKAIYAIAKNDGEAAMAVVNRQLAAKGVKADAPEKNVADAARAIPGAA
                     DVFLWDSVNWGEWALAYGKMAAVRQGAADRIRREATIAMMIDPKLDGGGPQRVLGRLH
                     DQTPHVPFLTGWASSKEAVRFLREANKIDPTNKITRVFLADALVSNDSKSAPEAIRIL
                     RDVINSPNDPNFEVESAQAQEDAKALLKEWGA"
BASE COUNT          723 a         1561 c         1376 g          968 t
ORIGIN      
        1 gacgatcgaa gcgccgggcg taagcgtcgt gaagaacacc ttcccgcttt ttccgcgcaa
       61 cggattgaga aagccgagca tggcgtcgtc gatgtaggcg atcgtcggcg cctgcggctg
      121 aaagatctgc gtcgcaatcg gcgtggcagg caagggtggc ggcggagccg gcaccggcac
      181 cttccctcgc ggcgtcttca ctgccggaac cttcgcttcc gcctccttcg ccatgcgggc
      241 gatctcccgg cgctgttcga tgacgtcgtg aatggcgccg atccaaccgg tgacgtacag
      301 catcacgatg acgaccgtga tccagttgaa gatgtgcttc tgccgcgcgt tcagcccgcc
      361 aaacacgcga tgccgcttcc gcgcacgcgg gaagaggtga tgttcagcca cacgtggaga
      421 ggttcacgat gtgtgccgtg gtggggtgtc gcgtttcggg ggtcgggagt cggagctggc
      481 gcgtgccggg cgagacccgg cccagggagt tcgcgtggga gaaacccaaa gcggttcccc
      541 acgcaagacg aatttcgatc tccgcccttc cagcacccgg gcgaggacgc ccgagtgccc
      601 gcggtcgaga cgaccgcact cctgcctact ccgacccccg accggcgacg cccgcaacac
      661 ctatactccc cgcgtgttgg acgagtctcg caaagatctc gccgcaggcg tcatcaggtc
      721 gctgctttgg atcgcggcgt tctgttttgc tgtcgggatc ttcctgacgg ccacgctgct
      781 gttgcgtggc gttccggcga cgccgcatgt ggctgtaggg cgggtcacgg tcgaaaacgc
      841 atcgaagctg cgcgactacc tgaccgtgct tctcttcttc atcatcgtcc cggtcgcgac
      901 gatcccgctg taccggctcg gcgcgcgcga gaatctgcgc ctgcgcgccg cggtcagcgg
      961 cgacggcgtt cgcaacctcg tttcggtgct cttcgtcgca ccgttttttc tcgcgccgtt
     1021 tctttacctc accacgttca aaccagcgtg gccgatcctc atccctctcg cgttttcgat
     1081 gctgctgccg cgcgcggtca tcacgtggca gcagactctc tggattcgca ggctcttcgt
     1141 acgcgaaatg gcgccgcatc acgcgctcat catcgtcgag gcgctttcct ggatcctctt
     1201 tcgatacatc gccgtcggta aacggatcgc gcacattccg acgctgttcc tcgagatcgc
     1261 gttcatcgcc ttcttcattg cccttttctg gctggcgttt ctgctgatcg ctcgcatcgt
     1321 cgccttcacc tccgacttca cgaccgagga ggccctgcag aggattggga ttgccgcgct
     1381 gcctctggtc atcctgccac cgctcgcgct cttgttcgtt caggggaacg tcgccatctc
     1441 gctggccatg gtgatgatcc ttctggcgct tccgctgacc cttagccgtc gcgaaccgat
     1501 cgattcgcgc agcgtccgca accttgtcgc cttcgccatc ctcccctcgc ttctcttctg
     1561 cctcagctac gcgtcgacgg cctcgctgac gcaatggatc gatctcttcc atcgcggtga
     1621 gacgctcggc cccgcatcgg atctgctgcg cggcaaagtg ccgttccgcg atgtgttcgc
     1681 gctgcacggg ctgctcgaag atggcctcct ggacacgtgg ctcatggagc tcttcgggcg
     1741 gaacgtcaac atcgttctcc tccgcccagt cgtcctcggc tccgttgcag tggcaatgct
     1801 ctggtacctc ggcatggtga tcttcgactc cattccgctc gcgatgcttt cgattctcct
     1861 cggcgccgta accacggtcg acaacgagcg cgccttgttc gagatcgtcg ccatcgcact
     1921 gctcatcggc gcgttgcggc ggaatgcccg atggcttgcg gttgcgagcg gagttgccgc
     1981 ggcgatcacg cttttctaca gcctcgatat cggtctctac accgttggcg gcggtgtgtt
     2041 ggcgatcctg gctgcgtctc tgctccgcaa tgcggcgcag cgcagttggg cattgctggc
     2101 aagttttgtc gccggaatcg cgctcggcgc agcacccttc gtcatctacc tcagcatgcg
     2161 cggggcgttc ggcgcgtttg tggagacatc gttctttgcc attcctcgca tcatcgacgc
     2221 catctggtcc ttgccgttcc ccgatctgac gacgaccttc cgcagcaacc tcaacctcca
     2281 ctcgttgtcc gacttcgtcc tcagcgatca cttccgtttc atcctgaatc cgctggtgat
     2341 cggcctggcg ctggtcgtgc tcgtgcagcg caggatcacc cgccgaagcg atgctctcga
     2401 cctcggcctg gccgttctca cagcgttcgc gatcctgacg caacgcagcg cgctgggccg
     2461 cgctgatttt cagcaccagt acttttcggc gttcctcatc ggaccgatga tcctcattct
     2521 gctcgtgcta ttcggccgcg ctgctgcggg catctggcac acgcacgagc aggccacgaa
     2581 agcgtttgtc gtgctggccg cggcgatgat gctgccggta ttcgccgtca ttctgtgggt
     2641 gcccgacatc atcaacatgc gtgtcgatga caccacccac tacctggggc gcgtaagccg
     2701 tatcggttgg atcgatccgg cggcagaaga gatccgcaat cgtgtcgaat cggtcaaatc
     2761 ggccgtagac gcgctcagca agccgggcca gccgatgttc gacttctcca atcagccggc
     2821 gctctatttc ttctgcgacc ggccgaaccc gacgcgtttt taccaggtgc cgatcctctc
     2881 cccgcgcgcc taccaggccg agacgatact cgcgctggag cgggcaaagc ctccgctcgt
     2941 catcaggcac tcaccgcagg aattcgacgt cttcgacggc atcgacaact cgatccgcgc
     3001 gcaggccgtg gccgcgtaca tcgatgacca ttacacgttc gcgcggacaa cccgaggagt
     3061 cgagatctgg cggcggagac cggaggcgac ggcgctgaat cttgcggcct acctgtcgcg
     3121 catccgcatt ccgacggtcg aagagctcgg cgcaatcggc gatcggtcac gcgtggtttt
     3181 tccgtccgtc ggcagtttgc ccggcgcgaa cgcgtcgtac tggcgaagcg atctcacact
     3241 gcacaatcca ttcaaggagc ggatgccgtt ggagctgcgc tacgtttcgg gcgattcgcg
     3301 catcgacaag aacgttacga tcctcggcgg acaaagcctg cgatcggagg atgtcgtccg
     3361 gtcgttcttt ggggcgccgg aaggaagcgg agtgctgtgg atcgaatacc gcgggcagca
     3421 ccaaccggtc gcgcggatca agacttacga cgccgcacac aacgcgcgtg gctcggtcag
     3481 tgcgccgttg tcgatgcgcg actctgccag cgcggggagc gagaaccagg acctcaccct
     3541 cgtcggcatt ccgaacggca ccgatcgccg cgtcaacatc ggcatcgtca acatcggaaa
     3601 agtccctgcg attttccgca tcactgtccg cacgcgaagc ggtcagaaga tcggcaagac
     3661 gttcgaggaa gcgctcaacg aagacgagtt gcgaacgatc cccgagatcg agaagacgct
     3721 cggcgtcacg ctcgacgaga ccagcgccct gcacatctcc atgatcgccg gcacctgtgt
     3781 cgcctacgcc agcgtcgtcg accccaacgg cgactcgcag ttcatgcccg cggtcgcgcg
     3841 ataacccttc gcgtcttcgc gtcttcgcgt gagacctacg cgccccactc tttcagcaac
     3901 gccttcgcgt cctcctgcgc ctgcgcgctc tcgacctcga aattcggatc gttcggggag
     3961 ttgatcacgt cgcgcaggat gcgaatcgct tcgggcgcac tcttgctgtc gttcgagacc
     4021 agcgcgtccg ccagaaacac gcgcgtgatc ttgttcgtgg gatcgatctt gtttgcctcc
     4081 ctgaggaaac ggacggcttc cttgctcgag gcccagccgg ttaggaatgg gacgtgcggt
     4141 gtctgatcgt gcagccggcc gaggacgcgc tgcggtccgc cgccgtcgag cttcggatcg
     4201 atcatcatgg cgatcgtcgc ctcgcggcgg atccggtctg cggctccctg gcgcaccgcg
     4261 gccattttgc cgtaggcgag agcccactcg ccccagttga cggaatccca gaggaagaca
     4321 tcggccgcgc cggggatggc ccgcgcggca tcggcaacgt tcttttccgg ggcgtccgcc
     4381 ttcacgcctt tcgcggcgag ctgtcgattg acgacggcca tcgccgcctc gccatcgttc
     4441 ttcgcgatcg cgtagatcgc cttcttatcg tcactggtcg atgcgacata cgcgcccttg
     4501 aatcgaaccg cccgcaacag ctttccgcgt gcttcgagat tgttcggctc ctgtgcaact
     4561 gccttgcggt aggcagcgat cgcggcatcg acctggcccg ccctggcccg gccgccctga
     4621 tggccttc
//
LOCUS       DATLVW010000724         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_239963, whole genome shotgun sequence.
ACCESSION   DATLVW010000724 DATLVW010000000
VERSION     DATLVW010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239963"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..410)
                     /gene="rnpB"
                     /locus_tag="VNN25_21070"
     ncRNA           complement(18..410)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNN25_21070"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(965..1396)
                     /locus_tag="VNN25_21075"
     CDS             complement(965..1396)
                     /locus_tag="VNN25_21075"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXH94081.1"
                     /translation="MSWVIDGNNVLGRAGAARFSVERKLELVRTLGAFARINRTKVAC
                     YFDGPEPEQFGKTPGSVSVIFSGERSADDLIIKRVATGSGWSVVTADRALGARIKRRE
                     VAIIDPGRFMNELESLPQVEEPTAEEEWLAWFSDEKNRNVF"
     gene            complement(1393..1950)
                     /locus_tag="VNN25_21080"
     CDS             complement(1393..1950)
                     /locus_tag="VNN25_21080"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HXH94082.1"
                     /translation="MITSLLTSAGLGLGAGINAYATLLVFGLVSRWQPAIFHDELARF
                     FASTPVLIVLVVLYLVEFAADKVPTVDHVWDVIHTIIRPIAGALVAWAAVSDKIPHGA
                     VILASVIAGSAALGSHLTKASVRGASTLSTAGIGNPILSLIEDAFAFVNAIIAIFLPW
                     LVILTLIGMPLIFFLVYQRFRSRQA"
     gene            complement(1950..>3060)
                     /locus_tag="VNN25_21085"
     CDS             complement(1950..>3060)
                     /locus_tag="VNN25_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94083.1"
                     /translation="PAPPPATSRSVWDEPEEDDHHSGTELAPPLPASVSAAALGSEPQ
                     WGFDEAQIEAARRAAVPVRAKDEVPSSVKPPVTKPPRKSRVGLWITILVLLLLGGAAY
                     PAWQWWQDRNAPEPIVPIKRAKLRNAGVPPAGPAASTPPTVATTSTVPVAALAAKTSS
                     SPTAPLTAPNTASAPTQSSPTTTIAAQKAPSHPATAITAPAPPLQVAKNTPPQKQPPP
                     AATTTAAPRTLPRTAAAAITPVPQPASTALSATHLERTAAGAPTITNTPSAGAAPTSD
                     ATRAKYDSMAKDFAARKGGTYTVQFELVCETASITRALSEAGDKAWFVPISYRGRPCY
                     RVFWGRFDTSAEAINASKTMPSGLSKAPVVVKIPR"
BASE COUNT          538 a          929 c         1008 g          585 t
ORIGIN      
        1 ccccaaaaaa gaacaacgtg tgaagtggac ctgtaagccg aattctgttc cccgtgccga
       61 agcaaggggt gaagatcatt cgtctgccac gcgcgttacc gcggtggtca agcaacctac
      121 ccggaagctc ataacggaga cgggcggctc catgacttcc ctatttggtc ttgctccgca
      181 gggggtttgc cgtgccgtcc acgcttgcgc ggaacgcggt gcgctcttac cgcaccgttt
      241 cacccttacc tcctcgcctt cgaccttgcg gcctcgggcg cgatcggcgg tctattctct
      301 gttgcacttt ccgtcgcgtc gccacgcctg gccgttagcc agcctgctgc ccgtgagagt
      361 tcggactttc ctcgacgcct acagagcgcc gcgatcctcc agtccactcc acacgatcga
      421 cgtaacagga ttgggattgg accattccgg gtgtcggaga acggcggggt cgggggtagg
      481 aggaagaaga tggctgtacc gccgaagccg ttgccgatac gcgcaaaacg tgatgtgtca
      541 tcctgagcct gccggaggcg cccgcgcaac acgttgctct cgcgcagatc atcctcggcg
      601 ccgcaggacg gcgaaggatc tcaaaatggg aagatctcgt tcgagcgcct tgaccatgtt
      661 cgctcgcgct ggacgaacac gatctgcgaa gtttgagatc cttcgccgtc ctccgccgcc
      721 tcgcacgcac tgcgcaaaca tcgcgattcg cgcaggcggc tgcggcagtc tcaggatgac
      781 accaatcttg attggactgg gaaggatgac agcggcccta ccctgcactt ctcacacttg
      841 tgggctgaaa catgtgagat gtgcggtcac cccgaccccc gccgtcctcc gactcccgca
      901 ctaaatcctt caacttaaat cgttgctgtt tcagtcgctt acaatcgatg tcggcgtgcc
      961 gacatcaaaa tacgtttcgg tttttttcgt cggaaaacca cgccagccac tcctcttctg
     1021 cagtcggctc ctccacttgc ggcagcgact ccagttcgtt catgaatctg cctggatcga
     1081 tgatggcgac ttcgcgccgt ttgatgcgcg cgccgagagc gcgatcggcg gtaacgacgc
     1141 tccagccgct gcccgtcgca actcgtttga tgattagatc atccgcggat cgctcgccgc
     1201 tgaagatcac gcttacgctg ccaggggttt tgccgaattg ctccggttcg gggccatcga
     1261 agtagcaggc cactttcgtc cggttgatgc gtgcgaaagc gcccagcgtt cgcacgagct
     1321 ccagcttccg ctccaccgaa aagcgcgcgg caccggcgcg cccaaggacg ttgttgccgt
     1381 cgatcaccca gctcatgcct ggcgactgcg aaaccgctgg tagacgagga agaagatcaa
     1441 cggcatcccg atgagcgtga ggatcaccag ccacgggagg aatatggcga tgattgcatt
     1501 cacgaacgcg aacgcgtcct cgatcagcga gaggatggga ttgccgatcc ccgccgtcga
     1561 gagcgtgctg gccccgcgaa cgcttgcctt ggtcaggtgc gagccgaggg ctgcacttcc
     1621 ggcgatcacc gacgccagga tcacggcgcc atgcgggatc ttgtcggata ccgccgccca
     1681 tgcgaccaga gcgccggcga tcgggcggat gatcgtgtgg atgacgtccc agacgtggtc
     1741 gacggtcggg actttgtcgg ccgcaaactc aacgaggtaa agcacgacca ggacgatcag
     1801 taccggcgtc gacgcgaaga agcgggccag ctcatcgtga aagatcgctg gttgccatcg
     1861 cgacacgaga ccgaaaacga gcagcgtggc gtaggcgttg atgcctgcgc cgaggccgag
     1921 tccagccgag gtcaggagcg aggtgatcat taccgtggga tcttgacaac caccggcgcc
     1981 ttgctcaggc ccgacggcat cgttttcgac gcgttaatcg cctcggcact cgtgtcgaag
     2041 cggccccaga agacgcggta gcaggggcgg ccgcggtaac tgatgggaac aaaccaggcc
     2101 ttgtcgccgg cctcactcaa agcgcgcgtg atcgacgcag tctcacaaac gagctcgaat
     2161 tggacggtat aggtaccgcc tttccgggcg gcgaagtcct tcgccatgct gtcgtacttt
     2221 gcgcgggtgg cgtccgaggt tggagcagcg ccggcgctgg gcgtgttggt gatggttgga
     2281 gcgcctgcgg ccgtccgctc gagatgcgtg gcgcttaaag cggtggatgc cggttgcggg
     2341 accggagtga tcgcagctgc tgcggtgcgc ggcagagtcc gaggagcggc cgtcgtcgtc
     2401 gcggcaggag gcggctgctt ttgcggcgga gtgttcttag cgacttggag tggcggtgcc
     2461 ggagcggtga tggcggtcgc ggggtgagaa ggagcctttt gggcggcgat tgttgtagtc
     2521 ggggaagatt gcgttggtgc ggacgcagta ttgggtgccg tgagcggtgc tgtcggggac
     2581 gacgaggtct ttgcggcgag cgcggccacc ggcacagtcg aagttgttgc cacggtcggc
     2641 ggcgtggacg ccgccgggcc agccggcggg acgccggcgt tccggagctt tgctcgcttt
     2701 atggggacga ttggctcggg cgcgttgcga tcctgccacc attgccaggc tgggtaggca
     2761 gctccgccga gcagcaggag tacgaggatc gtgatccaca gacccaccct gctcttcctc
     2821 ggcggcttcg taacgggcgg tttgacggag ctcgggacct cgtcctttgc gcgcaccggc
     2881 acggccgcac ggcgggcagc ctcgatctgc gcttcatcga aaccccactg cggctcggag
     2941 ccgagtgctg ccgcgctgac gcttgcggga agaggtggcg cgagctcggt accggagtgg
     3001 tgatcgtctt cttcaggctc atcccaaacg gacctggagg tcgccggcgg tggcgcaggt
//
LOCUS       DATLVW010000725         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_400443, whole genome shotgun sequence.
ACCESSION   DATLVW010000725 DATLVW010000000
VERSION     DATLVW010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400443"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /gene="ligD"
                     /locus_tag="VNN25_21090"
     CDS             complement(<1..1792)
                     /gene="ligD"
                     /locus_tag="VNN25_21090"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HXH94084.1"
                     /translation="MAQSPKALLQKLFPPMLATITEAPPRDGKSWAFELKYDGFRAVT
                     AIVDGSVAMWSRNKLDLAPRFPSVAAALSKLKLPEVVLDGEVVVLDAQGAPRFQLLQQ
                     GGRELYVVFDILWLDGQDLRKRSYEERRALLVKTLKRAPSSISVSEQLEGGGEAALER
                     AKKSGWEGIVAKRRTSTYEGRRSKEWLKIKAINEQELIVVGWNPSTHSAREIGSLHLG
                     VMGPDNELHYAGKVGTGFSYKQRVWWLEELSKDVVPKSLVKDAPRERVATWVRPRYVV
                     QVAFTEWTADNRLRHPSFLGIREDKRVGEVVREGEWGVGSGESEMKRGGSGVGSGESE
                     MKTKKTSEKPSRSSTKSPTAKPISAKSSKQVPSSKTSISHPPLPTPHPPVNLTHPERI
                     LYPRDGITKQDVADYYAAMTEPMLRALRDRPLALEHWNDGIDKPSWFHQDVGRGAPPW
                     VSSIETPTRTTGKSVRHLEVDRPETLRWLAQMSVLTIHMWASHAPSLEEPDWLVFDLD
                     PAKGKGIEQAIDAAIIIRGLLDHLHLPSLPKTSGKRGIHVLIPLAGGYTHEEANEFAC
                     SIGNAVTSKVPEFTMERALNKRQGRLYLDCM"
     gene            1850..2080
                     /locus_tag="VNN25_21095"
     CDS             1850..2080
                     /locus_tag="VNN25_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94085.1"
                     /translation="MSRYVKKFKSTTALGPTEERGFPLWVGYDIEGLDGDVSGLVNVD
                     DWSEERLREIGVTTFTRVEIGDVAMRGKLGID"
     gene            complement(2114..2563)
                     /locus_tag="VNN25_21100"
     CDS             complement(2114..2563)
                     /locus_tag="VNN25_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94086.1"
                     /translation="MARYIQRITLLGRQKWIGWEIDGVGTEIPGLILADELPEEDLRS
                     RGVKEFKTLPFETFLEMNPQSQQPAEGSLLDEDVEFRLDALQVGIDRLRGRGEDAVPK
                     ALLMHMFGNAVHLNHPRVIKRLQEWEQRGAVEFVGRDECYLRIVGKL"
BASE COUNT          463 a          851 c          835 g          531 t
ORIGIN      
        1 gcatgcagtc gaggtagagc ctcccctgcc gtttgttcag cgcgcgctcc atcgtgaatt
       61 cggggacctt cgacgtcact gcattgccga tggagcaggc gaactcgttc gcctcctcgt
      121 gcgtgtagcc gccggcgagc gggatgagca cgtgaatgcc gcgcttgccg gacgtcttcg
      181 gcagcgacgg caggtgtagg tgatcgagaa gaccgcggat gatgatcgcg gcgtcgattg
      241 cctgctcgat cccttttccc ttggccggat cgagatcgaa gacaagccag tcgggctcct
      301 cgaggctggg ggcgtgcgac gcccacatgt gaatggtcag caccgacatc tgcgcgagcc
      361 accgcaacgt ctcgggacga tcgacttcaa ggtggcgaac actcttgccg gtcgtgcgtg
      421 tcggcgtctc gatcgagctc acccacggcg gcgcgccgcg gccaacgtcc tgatgaaacc
      481 acgacggttt atcgatgccg tcattccaat gctcgagcgc cagcggccgg tcgcgcagcg
      541 cgcgaagcat cggctccgtc atcgccgcgt agtagtccgc gacatcctgc ttggtgatgc
      601 cgtcccgggg atagaggatg cgttcgggat gggtgaggtt gacggggggg tggggtgtgg
      661 ggagtggggg gtgggagatc gaggtcttgc ttgatggaac ctgctttgac gatttcgctg
      721 agattggctt cgcggttggt gacttcgtcg acgaccgtga cggcttttcg ctagtcttct
      781 tcgtcttcat ctccgactcc ccactcccga cccccgaccc cccacgtttc atctccgact
      841 ccccactccc caccccccac tccccctcac gaactacctc cccaactctc ttatcctccc
      901 gaatccccag aaacgaagga tgcctcagac ggttatcggc ggtccattcg gtgaaggcga
      961 cctgcacaac gtagcggggg cgaacccagg tagccacgcg ctcgcgaggc gcgtctttga
     1021 ccagcgactt cgggacgaca tccttcgaca gctcttccag ccaccagaca cgctgcttgt
     1081 acgaaaagcc ggtgccgact ttgccggcgt agtgaagttc gttgtcgggg cccatgacgc
     1141 cgaggtggag ggaaccgatc tcgcgggcgg agtgggtgga gggattccag ccgaccacga
     1201 tcagctcctg ctcattgatc gctttgatct tcagccactc cttcgagcgg cggccttcgt
     1261 acgtcgacgt ccgccgcttg gcgacgatcc cttcccatcc cgacttcttc gcgcgttcga
     1321 gcgccgcctc gccgccccct tcgagttgct cggagacgct aatcgacgac ggcgcgcgct
     1381 tcaatgtctt gaccagcagc gcgcggcgct cctcgtagct gcgcttgcga agatcctggc
     1441 cgtccaacca gaggatgtcg aagacgacgt acagctcgcg accgccttgc tgcaggagtt
     1501 gaaatcgcgg cgcgccttgc gcatcgagga cgacgacttc gccgtcgagc acgacctcgg
     1561 gcagtttcag cttcgaaagc gcggcggcga ccgacggaaa gcggggcgcc aggtcgagct
     1621 tgttgcggct ccacatggcc acgctgccat cgacgatcgc cgtcacggcc cggaagccgt
     1681 cgtacttcag ttcgaacgcc cagctcttcc cgtcgcgcgg cggcgcttcg gtgatcgtcg
     1741 ccagcatcgg cgggaagagt ttctggagca gcgctttcgg agactgtgcc atcgttgttg
     1801 ggttgctgcg tttctgcgtt gccggagatg ataggcaact tgcaaaccga tgtcccgcta
     1861 cgtcaagaag ttcaagtcca cgaccgcact tgggccgacc gaggagcggg gattcccgct
     1921 ttgggttggg tacgacatcg agggcctcga cggcgatgtt tcaggactgg tgaacgtcga
     1981 cgactggagc gaggagaggc tgcgggagat cggtgtgacg acgttcacgc gagttgaaat
     2041 cggagacgtt gcgatgcggg ggaagttggg gattgattga tcacgcgaag acgcgaaggc
     2101 gcgaagagca cactcacaac ttccctacga tccgcaggta gcactcatcc cgtcctacga
     2161 actccacggc gccgcgctgc tcccactcct gaagccgttt gatgacgcgg gggtggttga
     2221 ggtggacggc gttgccgaac atgtgcatca gcagcgcctt cgggacggcg tcttcgccgc
     2281 gtccgcgcag acggtcgatg ccgacctgaa gcgcgtcgag ccggaactcg acgtcctcat
     2341 ccagaagcga tccctcggcc ggctgctgcg actgcgggtt catctccagg aacgtctcga
     2401 acgggagagt cttgaactct ttcacgccgc ggctgcgcag atcctcttcg ggcagctcgt
     2461 cggcgaggat gaggcccggg atctccgtcc cgacaccatc gatctcccat ccgatccact
     2521 tctgcctgcc cagcaacgta attcgctgga tgtagcgcgc catgactcgt ctctccgccg
     2581 gcctcgcaac agaatatacg cctctgcgcg gtcattcgat tgtaacgagg agtgggtcaa
     2641 aaatggtgct acccttcgcc gctgcaattt ccctaacttc
//
LOCUS       DATLVW010000726         3012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_29712, whole genome shotgun sequence.
ACCESSION   DATLVW010000726 DATLVW010000000
VERSION     DATLVW010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29712"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VNN25_21105"
     CDS             <1..191
                     /locus_tag="VNN25_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94087.1"
                     /translation="IFLGLRQAGGVHYDDIVRLCGNGGIEWMERGLSDGWLRRVGARV
                     AFTSSGFLLSNEYISQLF"
     gene            203..910
                     /locus_tag="VNN25_21110"
     CDS             203..910
                     /locus_tag="VNN25_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94088.1"
                     /translation="MSLPAFAQDVKFDPNLVTQASFSQFSRIVGQAIFADPVQPARTS
                     GLLGFDAGIAATVVPIDTKAAYWRAAVPANSSFTTHGYVGVPRLVASKGFGFGTISGT
                     YAKVSNSGIKMWGGALDAPLLRGGVLKPELALRVSYSALSGVDVFREKTYGAEVFLSK
                     GFGPITPYAAVGIMRINAAGDVPATSRTSAFRLTDRSSFNRYTAGVRLSLLVPKLVVE
                     VTQAEVRSYAAKVSIGF"
     gene            complement(1003..2595)
                     /gene="pruA"
                     /locus_tag="VNN25_21115"
     CDS             complement(1003..2595)
                     /gene="pruA"
                     /locus_tag="VNN25_21115"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HXH94089.1"
                     /translation="MLTEFKNEPLTDFTKPEEKAAMEAALATVHAEFGREYPVVIGGE
                     QITGLKTYDSINPSHKSQLLGRFNKATKQHVEQALDAAWKAFESWKREPLEVRAGLLV
                     RAAALMRQRKHEFSATMVYEVGKTWPEADADTAEAIDFLEFYAREAYRWGGEHPITKI
                     ESEDNALVYVPLGVGGVIPPWNFPLAIMAGMTTAAVATGNCVILKPSSDAPWIAWRFF
                     ALLEEAGMPPGVVNFLSGSGAEVGDPLVMSPRLRFISFTGSKEVGLHINEEAAKSRPG
                     QVWIKRVVAEMGGKDAIIVDRDWKNLDEAATAVVASAFGFQGQKCSACSRLIVDDAIY
                     DKFVPMVVEKTKALRIGPAEDAESQVGPVVNESAMRKIKEFIEKGKSDGGKLLAGGNL
                     REGEGFYIEPTVIGDVAPDATISQEEIFGPVLAVTRAKDFDDALRIANNTQYGLTGAV
                     FTDDEAKLDRAREEFFVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLGLFL
                     QAKAISRKVGKQPRVVAEAEAF"
     gene            2780..>3012
                     /locus_tag="VNN25_21120"
     CDS             2780..>3012
                     /locus_tag="VNN25_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94090.1"
                     /translation="MYSKTLIAAIASVLSVLFWASCSISLPWAHEPIGQEINLAFVIR
                     NNLVFLPSATIDGRSGQFLLGTAAPHTVVDRAFA"
BASE COUNT          533 a          944 c          918 g          617 t
ORIGIN      
        1 ccatcttcct cgggctgcgg caggccggag gcgtacacta tgacgacatc gtacggctct
       61 gtggaaatgg ggggatcgaa tggatggagc gcggcttgag cgacgggtgg ttgcggcggg
      121 tgggagcgcg agtggccttc acctcttccg gtttcctgtt gagcaacgag tacatttcgc
      181 agcttttctg attgcgctgg ccgtgtcgct gccggcgttt gcgcaggatg tgaagttcga
      241 tccgaatctc gtcacgcagg cgagcttcag ccagttctcg cggatcgtcg gtcaggccat
      301 cttcgccgat cccgtgcagc cggcacgcac cagcggactc ctcggcttcg atgccggcat
      361 cgccgcgacg gtcgttccga tcgacacgaa ggccgcatat tggcgtgccg cggtcccagc
      421 gaacagcagc ttcacgacgc acggctacgt cggcgtgccg cgtctcgtag catcgaaggg
      481 attcggcttc gggacgatct ccggaacgta cgccaaggtc tcgaacagcg gcatcaagat
      541 gtggggcggt gcgctcgatg cgccactgct gcgcggaggc gtgctgaagc ccgagctggc
      601 actgcgcgtt tcctattcgg cgctgtccgg cgtcgatgtg ttccgcgaga aaacctacgg
      661 cgccgaggtg tttctcagca aaggattcgg cccgatcacg ccgtacgcgg cggtcggcat
      721 catgcgcatc aatgccgcgg gcgacgtccc cgccacatcg agaacctctg ctttccggct
      781 gacggacagg agcagtttca atcgctacac cgcgggcgtt cgcctctcgc tactggtgcc
      841 gaagctcgtc gtcgaagtga cgcaagcgga agtgcgaagt tacgcggcca aggtgagcat
      901 cgggttctga acacaaaaaa cagacaccac agagacacag aggtcacaga gtcgatctct
      961 gcgctctccg tgtctctgtg gttcagcttc tctaacaatt aatcaaaacg cctcggcctc
     1021 cgccacgacc cttggctgtt tccccacctt tcgcgagatc gctttcgctt gcaggaagag
     1081 accgaggtag tcgcggccgc cggccttgga atcggtgccg gacatgttga agccgccgaa
     1141 cggatgcacg ccgaccagcg cgcccgtgca tttgcggttg agatagagat tgccgacgaa
     1201 gaactcttca cgcgcacgat cgagtttcgc ctcgtcgtcc gtgaagaccg cgcctgttag
     1261 tccgtactgc gtgttgttgg cgatgcgcag cgcgtcatcg aaatccttcg cgcgcgtcac
     1321 ggccaggacc ggtccaaaga tctcttcctg cgagatcgtg gcatcggggg caacgtcgcc
     1381 gatgacggtc ggctcgatgt agaagccctc gccttcgcgc agattgccgc cggcgaggag
     1441 cttaccgccg tcgctcttcc ccttctcgat gaactccttg atcttccgca tggccgactc
     1501 attcactacc ggccccacct gcgattccgc gtcctcggct ggaccaatgc gcagcgcttt
     1561 cgtcttctcg accaccatcg gcacgaactt gtcgtagatg gcgtcgtcga cgatgagccg
     1621 ggaacaggcc gagcacttct gtccctggaa tccgaacgcc gaggcgacga cggcggtcgc
     1681 ggcctcatcg agattcttcc agtcgcggtc gacgatgatc gcgtccttgc cgcccatctc
     1741 cgcgacgacg cgcttgatcc agacctgacc cggacgcgat ttggccgctt cctcgttgat
     1801 atgcagtccc acttccttcg aaccggtaaa cgagatgaag cgaagacgcg ggctcatgac
     1861 gagcgggtcg ccgacctcgg cacccgagcc gctgaggaag ttgacgacgc cgggcggcat
     1921 gccggcttct tcgagcagcg cgaagaagcg ccacgcgatc cagggcgcat ccgacgacgg
     1981 cttgaggatg acgcagttgc cggtggcgac agcggcggtg gtcataccgg ccatgatcgc
     2041 gagagggaag ttccacggag gaatcacgcc gccgacgccg agggggacgt aaacgagagc
     2101 gttgtcctcg gactcgatct tcgtgatcgg atgctcgccg ccccagcggt acgcttcccg
     2161 tgcgtagaac tcgagaaagt cgatcgcctc ggcggtgtcg gcgtcggctt ccggccacgt
     2221 ttttccgact tcgtacacca tcgtcgcgga gaactcgtgc ttgcgctgcc gcatcaacgc
     2281 cgcggccctg acgagcagcc cggcccggac ttcgagcggt tcgcgcttcc atgattcgaa
     2341 cgccttccac gcggcatcga gcgcctgctc gacgtgttgc ttcgtcgctt tgttgaaacg
     2401 cccgaggagc tgggacttgt gggaagggtt gatcgagtcg taggtcttca ggccagtaat
     2461 ctgttcgccg ccaatgacga ccgggtattc gcggccaaac tcggcatgca cggtggccag
     2521 cgcagcttcc atcgctgctt tctcctccgg tttggtaaag tcggtcagcg gctcgttctt
     2581 gaattcagtc agcatcgttc ctccgggtgg gtctttatgt tgcagagatc gggagtcaga
     2641 gatcagggac tagagatctg cgatcaggaa atggagaccg cgaaaccgtg acaaatcgag
     2701 atcagggtca agaaatgccg cgtggtctcc gatccctgat ctcttgtctc tgacacctgt
     2761 ttcctgatct ctgcttttta tgtactcgaa aactctcatc gccgcaatcg ccagtgtgct
     2821 cagcgtgctc ttttgggcat cctgctcgat ctcattgccg tgggcgcatg aaccgatcgg
     2881 ccaggagatc aacctcgcct tcgtcattcg gaacaatctc gtattcctcc cttcggcgac
     2941 catcgacggc cggagcggac agttcctcct cggcaccgct gcgccgcaca cggtggtcga
     3001 ccgcgcgttc gc
//
LOCUS       DATLVW010000727         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_74643, whole genome shotgun sequence.
ACCESSION   DATLVW010000727 DATLVW010000000
VERSION     DATLVW010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74643"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VNN25_21125"
     CDS             <1..423
                     /locus_tag="VNN25_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXH94091.1"
                     /translation="VDARVVQVDRKSGAIRDDAGVRAKFGVAPALIPDYLALIGDAAD
                     GYPGIPRIGAKTAADLIKRYGPIDKFPASVLEGERRELAMLFKNLATLRTDAPLFDDV
                     EELRWQGATSSFAAMAEKIGDARLAAKVLTTEAQRSQR"
     gene            complement(469..876)
                     /locus_tag="VNN25_21130"
     CDS             complement(469..876)
                     /locus_tag="VNN25_21130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH94092.1"
                     /translation="MTTVQRIVMDTSAYSHMRTGHQELTELLALAEALLIPVTVLGEL
                     EGAFELGSRASINRRVLTEFLSEAFVSILPTTPDVARRYGQIFARQRRAGRPVSVNDM
                     WIAASAIDSGGHLVTFDKDFGHIEALDCTILAP"
     gene            complement(873..1115)
                     /locus_tag="VNN25_21135"
     CDS             complement(873..1115)
                     /locus_tag="VNN25_21135"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HXH94093.1"
                     /translation="MAKQMTIRGIPEDVAGKLRRISKRHGRSVNTTVLEILRQAVGSD
                     ARRERLARYATWTSEDLAQVEEAVAAQRVIDESLWR"
     gene            complement(1240..2313)
                     /locus_tag="VNN25_21140"
     CDS             complement(1240..2313)
                     /locus_tag="VNN25_21140"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXH94094.1"
                     /translation="MKPRLQLLVVDDSAVMRQAMIAICRRDPGIEVSVAADPLIARNK
                     MRSSRPDVILLDIEMPRMDGLTFLRQLMATDPIPVVICSALAGRGTQTALKALEEGAV
                     EIVAKPRVALHDFLNESTEMLIETIRAAAHARLLPRRPAMIIEKRHSPDVILPAARAS
                     LARTTRKVVAIGASTGGTEALREILESMPGDAPGMVIVQHMPEVFTAAFAQRLNQSCR
                     MEVKEAADGDRIVDGRALIAPGNRHMLVHRDGAEYCVELNDGPLVSRHKPSVDVLFRS
                     VAQSAGSNAIGVILTGMGNDGSTGLLEMHTAGAVTFAQDEASCAVFGMPREAIACGAV
                     QSVHSLSAMGGAILTANERMRGR"
     gene            complement(2326..>2586)
                     /locus_tag="VNN25_21145"
     CDS             complement(2326..>2586)
                     /locus_tag="VNN25_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94095.1"
                     /translation="GNGWSVWLNPRLTKGWELLLRHDEMKPDSSTAQQRRRNIAGVAY
                     WIPNLQKVSSSVLLDYDSLDQQNYSVPRPRDTRVGLKMLVQF"
BASE COUNT          461 a          777 c          849 g          499 t
ORIGIN      
        1 gtggacgcgc gtgtcgttca ggtggatcgc aagagtggag cgatccgcga cgatgccggc
       61 gtccgagcaa agttcggagt agcgccggcg ctgattcccg actacctggc gctgatcggc
      121 gacgccgcgg acggctatcc cggcattccg cggatcggcg cgaagacggc cgccgatctg
      181 atcaagcgct acggtccgat cgataagttt cccgcttcgg tcctggaggg cgagcgccgc
      241 gaactggcga tgctgttcaa gaatctggca acgttgcgaa cggacgcgcc gctctttgac
      301 gatgtggaag agctgcgctg gcagggagcg acatcgtcat tcgcggcgat ggcggagaag
      361 attggggatg cgcggttggc cgcaaaagtc ttaaccacag aggcacagag atcacagaga
      421 tgaagcgatt cctctgtgtc ctttgtgtct ctgtggtcgt tttctcaatc aaggtgccag
      481 aattgtgcaa tcgagcgctt caatgtgacc gaagtctttg tcgaaggtca cgaggtggcc
      541 tccactatcg atcgccgacg cggcgatcca catatcgttc accgaaacgg gtcgcccggc
      601 acgcctttgg cgggcaaaga tctgaccata tctgcgggca acgtccggcg tcgtcggcag
      661 gatcgagacg aaggcctcgg aaaggaactc ggtcagaaca cggcggttga tgcttgcacg
      721 cgagcccagc tcgaaggcgc cctccaattc gccaagcacc gtcactggaa tgagaagcgc
      781 ctcggctaac gcgagcagct cggtcagctc ctgatgtccg gtgcgcatgt gggagtacgc
      841 cgaggtgtcc atgacgatcc gttgcacggt cgtcaccgcc aaagcgactc gtcaatcaca
      901 cgttgtgcgg cgacagcttc ctcgacctgc gcgaggtctt cagacgtcca tgtggcatag
      961 cgcgccagtc gttcgcggcg ggcatccgag ccgacggcct gacggagaat ctcgagaacc
     1021 gtggtgttca cgctccggcc atgacgctta ctgatgcgcc gaagtttccc ggcaacatct
     1081 tccgggatgc cgcgaatcgt catctgcttt gccatgcctg catgctagca cgattgtgcc
     1141 tgcattcctg aggagaatca gcaaccacag aggcacagag atcacagaga tgaagcgatt
     1201 cctctgtgtc ctctgtgtct ttgtggtgaa atgcttttct caccttcccc gcatccgctc
     1261 atttgccgtc agaatcgctc ctcccatcgc gctgagcgag tggacggatt ggactgcgcc
     1321 gcaggcgatc gcttcgcggg gcattccgaa tactgcgcat gatgcttcgt cctgggcgaa
     1381 ggtgacggcg ccggcggtgt gcatttcgag caggccggtc gatccgtcgt tgcccatgcc
     1441 ggtgaggatg acgccgatgg cgttgcttcc ggcggattgc gccacggagc ggaagaggac
     1501 gtcgacgctc ggcttgtggc gcgagaccag cggcccgtca ttgagctcga cgcaatactc
     1561 cgcgccgtca cggtggacga gcatgtggcg attaccggga gcgatcaggg cgcggccgtc
     1621 gacgatgcgg tcgccatccg cggcttcctt cacctccatg cggcagctct gattcaaacg
     1681 ttgtgcgaac gcggcggtga agacctcggg catgtgttgc acgatgacca tgcccggcgc
     1741 atcgcccggc atgctctcga ggatctcgcg cagcgcctcg gtgccgccgg ttgacgcgcc
     1801 aatggccacg actttgcgcg tcgtgcgtgc gagcgacgcg cgcgcggcag ggaggatgac
     1861 gtcgggggag tgccgcttct cgatgatcat cgctggccgc cgcgggagaa gccgggcgtg
     1921 cgcggcggcg cggatggtct cgatcagcat ctcggtcgat tcgttgagga agtcgtgtag
     1981 tgcgacgcgc ggcttggcca cgatctccac cgcaccttcc tccagcgcct tgagcgccgt
     2041 ctgcgtgccg cgtccggcca gcgccgagca gatgaccacc ggaatgggat cggtggccat
     2101 cagctggcga agaaacgtca ggccgtccat gcgcggcatt tcgatgtcga gcaggatcac
     2161 gtcgggccgg ctgctgcgca tcttgttgcg ggcgatgagc ggatcggcgg cgacgctgac
     2221 ttcgattccc gggtcgcggc ggcagatcgc gatcatggcc tgacgcatga ccgccgaatc
     2281 gtcgacgacg agaagctgca gccgcggttt catggttgcc ggccgttaga actgcacgag
     2341 cattttcagt cccacgcgcg tgtcgcgtgg acgcggcacg ctgtagttct gctggtcgag
     2401 cgagtcgtag tcgaggagaa cagaggatga caccttctgc aggttcggta tccaatacgc
     2461 cacaccggcg atgttccggc ggcgttgctg ggccgtggac gaatcgggct tcatctcgtc
     2521 atgccgcaac agcagttccc aacctttggt cagacgggga ttgagccaca ccgaccagcc
     2581 gtttcc
//
LOCUS       DATLVW010000728         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_608894, whole genome shotgun sequence.
ACCESSION   DATLVW010000728 DATLVW010000000
VERSION     DATLVW010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608894"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VNN25_21150"
     CDS             <1..735
                     /locus_tag="VNN25_21150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH94096.1"
                     /translation="VPSRAFSLRQCPADFVAMSFYKLFGYPTGIGALVARREALARLL
                     RPWFAGGTVLFASVAADTHRLRPRHEAFEDGTPDFLGIAGLDAGFDVLAEVGMDRLSA
                     HVSTLMRTLLDQLRSLRHRNGAPLVKIYGPSNTIDRGGTVAFNVCDREGVPLSYTLVE
                     TRARQAGVSLRGGCFCNPGASESAFSLDPARITTCLADLGATFTPERFAACSGTAVGA
                     IRASIGLANNRDDIQRAVDVVASFGW"
     gene            complement(763..1170)
                     /locus_tag="VNN25_21155"
     CDS             complement(763..1170)
                     /locus_tag="VNN25_21155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH94097.1"
                     /translation="MANAVIADASVLIALISRRDRHHAWAVSQARQWPAPWLTCESAL
                     SEAFFLCGITGRKALTDFVRSGLVKLQFDLATEIDPVLALMEKYRDVPMSLADACLVR
                     MTEIVAEPIVLTTDSDFRLYRRHSRQVVPSVMP"
     gene            complement(1148..1402)
                     /locus_tag="VNN25_21160"
     CDS             complement(1148..1402)
                     /locus_tag="VNN25_21160"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXH94098.1"
                     /translation="MKSLTVRLPDAMAAEIESESIADGISKSDVVRRRLENVSARPAT
                     LFDLAADLIGSVDDARMPRDLSSRKKNYLKSKGYGKRGHR"
     gene            1522..1860
                     /locus_tag="VNN25_21165"
     CDS             1522..1860
                     /locus_tag="VNN25_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HXH94099.1"
                     /translation="MRTIALTVTLLAISNLFMTFAWYAHLRNLSLKPWYIAATISWGI
                     AFFEYMVQVPANRIGFQRLSLMQLKVIQEVVTLCVFVPFAVLYMKQTVNLNFLWAGLC
                     LIGAVFFMFR"
     gene            complement(1873..>2014)
                     /locus_tag="VNN25_21170"
     CDS             complement(1873..>2014)
                     /locus_tag="VNN25_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HXH94100.1"
                     /translation="PVIFLTAKVQESDRQRFMQMGVRGIIAKPFDPLTLGQQIKTLLS
                     WN"
BASE COUNT          373 a          608 c          605 g          428 t
ORIGIN      
        1 gtaccgagtc gagcgttcag cctccggcag tgtcccgctg actttgtggc gatgtcgttc
       61 tacaaactct tcggatatcc gaccggcatc ggcgcgctcg tggcgcgccg cgaggcactg
      121 gcccggctgc tccgtccgtg gttcgcggga ggaacggtcc tgttcgcgtc ggtcgccgca
      181 gatacgcatc gtctgcgtcc gcgtcacgaa gccttcgaag acggcacgcc cgacttcctc
      241 ggcatcgccg gactcgacgc aggattcgac gtgctcgcgg aagttgggat ggatcgcttg
      301 tccgcgcatg tgtccacgct gatgagaacg ctgctcgatc aactccgctc tctgcgccac
      361 cgcaacggcg cgccgctagt gaagatttac ggcccatcga acacgatcga ccgcggcggc
      421 accgtcgcgt tcaatgtctg cgatcgcgag ggagtgccgc tgtcgtacac gctcgtcgag
      481 acgcgcgcac gccaagcggg cgtgtcgctg cgcggcggtt gtttctgcaa cccgggcgcc
      541 tcggaatccg cattcagcct tgaccccgcg cgcatcacca cctgcctcgc agacctcggc
      601 gccaccttca ccccggaacg tttcgccgca tgcagcggca ccgcagtcgg cgccatccgc
      661 gcctcgatcg gcctggccaa caaccgcgat gacattcaac gcgcggttga tgtggtggct
      721 tcgttcgggt ggtgacgcga gcgcacgcgg gtgatccaag cctcacggca tcacagatgg
      781 aacgacctgc ctgctgtggc ggcgatagag acgaaaatcc gaatccgtgg ttagcacgat
      841 cggttcagca acgatctcgg tcatgcgaac gaggcaggca tcggcaaggc tcatcggaac
      901 gtcgcggtac ttctccatca gcgcgaggac gggatcgatt tcggttgcga ggtcaaattg
      961 aagcttgacg aggccactcc gaacgaagtc ggtcaatgcc ttcctgccgg tgattccaca
     1021 gaggaagaac gcttccgata gcgcggactc gcaggtcagc caaggtgccg gccactgtcg
     1081 agcctgtgat accgcccaag cgtgatgccg gtctctccgc gaaatcaggg cgatgagcac
     1141 actggcatca gcgatgaccg cgtttgccat agcccttgct tttcaagtag ttcttcttgc
     1201 gcgaggaaag gtccctcggc attcgagcat catctacgct tccgatcagg tcggcagcga
     1261 ggtcgaataa tgttgctgga cgagctgata cgttctccag ccgccgacga acgacatcgg
     1321 acttggagat gccatcagcg atcgattcgg attcgatctc agccgccatc gcatcaggca
     1381 aacgtacggt gagtgacttc attcctggaa tgtattacaa gagcgccagc gctgcaagac
     1441 accctgtggt tgagacgagc tcgtgaacaa cgagccgcca tcgaaccgga gtatcgtccg
     1501 ccgaactttc aggaggattc cttgcgaacc attgcgttga ccgtcacctt gctcgcgatc
     1561 tccaacttgt tcatgacgtt cgcctggtat gcgcatctca ggaacttgtc gttgaagccg
     1621 tggtatatcg cggccaccat cagttggggc atcgcgtttt tcgagtacat ggtgcaggtg
     1681 ccggcgaatc gcatcggctt ccagcggctt tcgctgatgc agttgaaagt cattcaggaa
     1741 gtcgtcacgc tttgcgtctt cgttcctttt gcggtccttt acatgaagca gacggtcaac
     1801 ctcaatttct tgtgggccgg cctttgcctc attggcgcgg tgttcttcat gttccggtga
     1861 ccgtggaaac gctcagttcc aggacaggag ggtcttgatc tgctggccga gggtgagcgg
     1921 gtcgaagggc ttggcgatga ttccgcggac gcccatctgc ataaatctct gcctgtcgga
     1981 ttcctgaact tttgccgtga ggaagatgac gggg
//
LOCUS       DATLVW010000729         9518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_279880, whole genome shotgun sequence.
ACCESSION   DATLVW010000729 DATLVW010000000
VERSION     DATLVW010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279880"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..895
                     /locus_tag="VNN25_21175"
     CDS             335..895
                     /locus_tag="VNN25_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94101.1"
                     /translation="MNNNNEVSQTNAASAAEDPAVTPESVVEQLRALRSQIPNYGQLP
                     VRAAQSLRRVSSLNPEFVQAAIESVGASATVQLTVGQTAEELQTATEAAARWSTVRDE
                     LKATLDGVASAVLTMKHTLGQSLLLTYTVSKKLIKMPQHADLLPHVALMRKTNRRGHS
                     RKTQPPVPLPTPMAEEAPEPSSTPIV"
     gene            957..1343
                     /locus_tag="VNN25_21180"
     CDS             957..1343
                     /locus_tag="VNN25_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94102.1"
                     /translation="MIKEKIPVAGLLNILARIRRERLAQGIAPARVPSEDTAFTDPFL
                     FGDEESMEYFELQTLLDALDSFPGEPVTFADVRDAALAEVLEHYYIAEKSVRERGPGH
                     ELLIPFVEEVRAMYRQDFGTEVPEKD"
     gene            complement(1467..1868)
                     /locus_tag="VNN25_21185"
     CDS             complement(1467..1868)
                     /locus_tag="VNN25_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH94103.1"
                     /translation="MIFVDSNIPMYLVGSPHPNKIAAEQTVAALVSRREKLVSDAEVL
                     QEILHRYAAISRLDAIQPAFDALLGLVDEVFPIEPENVQRAKTVLLGKYRLSARDAIH
                     LAVMEQHGVQHILTFDQGFDRYPNVRRVTNA"
     gene            complement(1865..2119)
                     /locus_tag="VNN25_21190"
     CDS             complement(1865..2119)
                     /locus_tag="VNN25_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HXH94104.1"
                     /translation="MSKRLQVILEDGEMKEIRTIARRRRMTVAAWVRGALRAAKSEEP
                     TSDPQRKMHALRVATSHSFPTADIEEMLAQIEKGYSEGSE"
     gene            2623..3477
                     /gene="rhlP"
                     /locus_tag="VNN25_21195"
     CDS             2623..3477
                     /gene="rhlP"
                     /locus_tag="VNN25_21195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04179.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HXH94105.1"
                     /translation="MARRIFITALLGLVLLLGCSFARQVHRRSNLMSFLYPRATEAPQ
                     PSAQGVRLRLPLRVGIAFVPSEAAAGPYGPYGVGTAALPGTVEKSLLNVLRDAFKSRD
                     WVKEVVVIPSTYLTPGGGFANLDQVSQMFGVDVIALVSIDQFQSSDPYPVSFLYLSIV
                     GAYVLPLDHNDTRTLIDAAVFYVPARRFLLRAPGQSRITGHSTAVDVSESMRERSVKG
                     LSMAMTDLSANLSSETDRFKQDIISGERNDVDVVNKQGTSIRTSGSIGGLWALSLLLV
                     VAMRRLWS"
     gene            3474..4001
                     /gene="rrtA"
                     /locus_tag="VNN25_21200"
     CDS             3474..4001
                     /gene="rrtA"
                     /locus_tag="VNN25_21200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HXH94106.1"
                     /translation="MNAELFALTPAAAHGEIWRLWTGHLVHYDFRHFLTNAIAIAVPI
                     ALIDRRSRRSILLSMTLLAPMISVALLAGAHFDEYRGASALALAVWVASALTLLRGQS
                     ASDRHTSAALIALAIGKLALETAGAGHLWTEVAPLPLAHAAGVIAGVAAFIAVTLIPT
                     PHSQHPTPRHAVQQS"
     gene            3998..4600
                     /locus_tag="VNN25_21205"
     CDS             3998..4600
                     /locus_tag="VNN25_21205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXH94107.1"
                     /translation="MTKSTHTRLGVPTRTAEDWEEAALDVIAARGIAAVSIPELARTL
                     DVTKGSFYWHFAGLDDLVSRAVARWDRLDKETLDQIRTIEDSVVRLRTLFAEAMSAQR
                     AQSLYLALAVSPSPDIGAALRKVSSRRLKLLAATYQELGMTEPAAQQQALLAYSAYIG
                     GVHLRNNDAPWLRQTRDVQAYVEHASTALIEPARKRKTKG"
     gene            4821..6860
                     /locus_tag="VNN25_21210"
     CDS             4821..6860
                     /locus_tag="VNN25_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXH94108.1"
                     /translation="MTVPTPPATVKKPHVTKIHGDTLVDNYFWLREKSNPDVIAHLEA
                     ENAYTAAMTADTQPLQQKLYGEMLGHVKQTDVNVPYLLGGYYYYSRTEEGKQYPIYAR
                     KKGSLDAAEEITLDLNELAKGLKFLSLGAYRVSDDGNLLAYSTDTTGYRQYTLVIKDL
                     RTGTLLPEKIERVDTVMWATDNKTIFYVTEDAVTKRSDSFYRHVLGTPANDLVFHEPD
                     ELYDIGAFRTRDKAFIVVESQSKTTSEARYLPANKPAEALRVVVPRTSDHKYFVDHRG
                     DLFYIRTNDHAKNYRLVTAPDSAPEMKNWKEIIPARADVKLDDFDLFKNHMAVSELKG
                     GSQTIRTVDLKSGQSTPIAFPEPVYSVFLDTNPEFDTATLRYRYQSLVTPSSVFDYDM
                     GTHASTLLKRFEVPNYDASQFASERIYATAADGTKVPVSLVYKKPLVRDGKRPMLLYA
                     YGSYGSPTWPTFNSNRLTLLDRGVIYAIAHIRGGGELGEPWREAGRMMVKRNTFTDFT
                     DVVENLVKSKYTSSDRLVIQGGSAGGLLMGAVVNMRPQLFKAVVAQVPFVDVINTMLD
                     ASLPLTTSEYLEWGNPNIKKEYDYIKTYSPYDNIAAKAYPAMLVKVSLNDSQVPYWEG
                     TKFVAKLREMKTDSNPLLLKVNMGAGHGGSSGRYDALRETAADQAFMLKAMGISE"
     gene            6894..8432
                     /locus_tag="VNN25_21215"
     CDS             6894..8432
                     /locus_tag="VNN25_21215"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HXH94109.1"
                     /translation="MIVLAATGGAYGAEFNITNSNDSGTGSLRWAIEQANVSPDGGYI
                     YGTGNVVHLTSPLPAITGGVYLSHVTLEGGGAGPGDGLVLDSEYDSIYDVHVNNFKGD
                     GIVIRGVADLALVSATGNQNGIRIEGRYSFIEATTATLNSANGIWITSTGSGNRLGGQ
                     DPDFCFGEGDGCFIPIGAGVWVGGNGGSGVRIDGDGNTLNNAHIGRDAPLNLGLPSRP
                     LANGGDGIVVIGAHNTVMSSTVSNSRGYGLLLLALANLEDNAGSCNARGFVSGPLIDP
                     PRITFARADPTAITFCVLFQGTPNARYRIGLAAATTACPDFGLPSLGTTTVTTDATGY
                     ASWTATLGTGFTVPGVSAIATRGEAEVSRQSDIVAAFATREPRADLTVQTTAPGGASA
                     TDLIDFVTTVTNIGPAAVDGFIVDIPRTPGTAFLSAATTSGACYLGGLQECFIETLAV
                     GESATIRERVRVLATSGTLHHLATVRHYDSTRSQPRVDPNPANNTSTVDVRIITPRHH
                     ATGR"
     gene            complement(8511..9236)
                     /locus_tag="VNN25_21220"
     CDS             complement(8511..9236)
                     /locus_tag="VNN25_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH94110.1"
                     /translation="MRALDSKLFVIRADLGPRAPRDYRLDVPAMRGFEGIEFRKPVTF
                     FVGENGSGKSTIIEALAVAAGFNEEGGSRNFRFETVRRDEEGERPLFFHITRSARYPS
                     DGYFLRAESFFNVATEIDRLGVTRGYGGVSLHEQSHGEAFLALLVNRFLGDGLYILDE
                     PEAALSPARQLALLARVHELVKHGSQFLIATHSPIVMAYPDATILLLDDGMREVAYEE
                     TDHYLVTRDFLNHRKKMLDELMS"
BASE COUNT         1919 a         2961 c         2864 g         1774 t
ORIGIN      
        1 tccggccacg cagaggcgac gcctgagtca tagcgtccga ttcccgagcc agagaccggt
       61 gggaagtaga ggaccggcct tgaatgaggt gggattccgt cgagcttgag ccatggcgag
      121 ccggaatccc cgagaagtgg ggttgacgcg ttagctcccc cagcctcgtc acaacatccg
      181 ttcccccgag gatcgctacc tttgggggca ggccgggcgg acggtttgcg acgccgccct
      241 tcgacattcc acccgggaac ggcgcccgga ttgcgcacac agcccgaccg ccgtcccatt
      301 accgattgcc cttcggggca gaaaggagat caccgtgaac aacaacaatg aagtcagtca
      361 aacaaacgcc gcgtcggcgg cagaggatcc ggcggtgacg ccggagtcgg tggtggagca
      421 actccgcgcg ctgcggagcc agatccccaa ctacggacag ttgccggtac gggcagcaca
      481 gtcgctgcgg cgggtgtcga gtctcaatcc ggagttcgtg caggcggcga tcgagtccgt
      541 cggcgcgtcg gcgacggtgc aattgaccgt cggtcagacc gccgaggagc tgcagaccgc
      601 caccgaggcg gctgcgcgct ggtcgacggt ccgcgatgag ctgaaggcaa cgctcgatgg
      661 cgtcgcctca gcagtcctga ccatgaagca cacccttggc cagtcgcttc tgctgaccta
      721 caccgtcagc aagaagctga tcaagatgcc gcagcacgcc gatctgctgc cgcacgtagc
      781 cctcatgcgg aagacgaacc gccggggaca cagccgcaag acacagccac ctgtgccgct
      841 gcccactccg atggccgagg aggccccgga accttcgtcc acgccgatcg tctaaagcaa
      901 taaccctggt cagccgggcg tgccatcgag gcgcgcccgg cattttggag gattcgatga
      961 tcaaagagaa aatcccagtg gcaggcctcc tcaacatcct ggcgaggatc cgaagagagc
     1021 ggctagcgca gggcatcgct cccgcccgcg tcccttccga agatacggcc ttcacggacc
     1081 cattcctctt cggcgacgaa gagtccatgg agtatttcga gttgcagacg ctgctcgacg
     1141 cgctggactc attccccggc gagcctgtga cgttcgcgga tgtccgtgat gcggcactgg
     1201 ccgaggtgct ggagcactac tacatagcgg aaaaaagtgt cagggaacgg ggacctgggc
     1261 acgagctcct gatcccattc gtggaggaag tgcgagcgat gtaccgccag gatttcggga
     1321 cagaggttcc ggagaaggat tgagcgttcg tgcgttgtct tccccttcgc gttcttcgcg
     1381 gcttcgcgtg ataggtgtgt tgacgcgaag gcgctcacgc gaaggcgcga agacgcgaag
     1441 agcggaggaa caacttcagt tctgagtcag gcgtttgtga ctcttcgaac atttggatag
     1501 cggtcgaacc cttgatcgaa ggtgagaatg tgctggacac catgctgttc catcacggcg
     1561 aggtggatgg cgtctctcgc agacaagcgg tacttgccta acaggacggt cttcgcacgc
     1621 tggacgtttt ccggctcgat cggaaagacc tcgtcaacca ggccaagcaa ggcatcgaag
     1681 gcgggctgaa tcgcgtcgag cctgctgatc gccgcataac gatgaaggat ttcctgaagc
     1741 acttctgcgt cgctaaccag tttctcccgc cggctgacca gcgcagcgac cgtttgctcg
     1801 gcggcgatct tattgggatg cggagatccc accaggtaca tcgggatatt cgagtcgacg
     1861 aagatcactc agacccttca gagtagcctt tctcgatctg ggccagcatt tcctcgatgt
     1921 ccgccgtcgg aaacgaatgg gaggttgcca cgcgaagagc gtgcattttt cgttgcggat
     1981 ctgaggtggg ctcctctgac ttagccgctc gaagcgcgcc tcgcacccac gcagcgaccg
     2041 tcatccgtct gcgccgggca atcgtgcgga tctctttcat ctcaccatct tcgaggatga
     2101 cttgaaggcg cttactcatg atgatgagta taacggatgc cgggtatgag gtagtccccg
     2161 cgtgacaagc tctcctcgct gccgcggtaa gatctgccac tcttttggcg gatgaagagc
     2221 gtttgatcaa aggtacccat gaagctacgc gaaaaacctg agtcgacctc gggaacgctt
     2281 gggcaattgg agcccgtgaa ggttcggttg cgggtaaggg atctgacaag aatcatccgg
     2341 tggaccggct tttcggcgct ctgacgctcg gcggaccggt tgacgaggtc gttgaccaga
     2401 tgcgaggtcc tcggcccgct caccgtagga agaaacgctc ggcgccaccg cggtgacatc
     2461 gcggccgcaa ggtccctcct cactccaccg cggcctcggt aatcgcactc caattcacgc
     2521 gcgcgatcat ttggaccttc catacctatc ggtattgaca agtttcgtcc gccgacttat
     2581 cttccatacc gacaagtacg gtacgaacag ttggaggctc tcatggcacg ccgcattttc
     2641 atcactgccc ttctcggatt ggtcttgttg ctcggatgca gtttcgcgcg tcaggttcat
     2701 cggcgcagca atttgatgtc gtttctctat ccgagggcga cggaggcgcc gcagccttcg
     2761 gcgcaggggg tgcggctgcg gttgccgttg cgggtgggga ttgcgtttgt gccgtccgag
     2821 gcggcggccg ggccgtacgg gccgtacggg gtagggaccg cggcgctgcc ggggaccgtg
     2881 gagaagagcc tgctgaacgt cttgcgtgat gcgttcaaat cgcgggattg ggtgaaggag
     2941 gtcgtggtca ttccttcgac ctatctgacg ccgggaggtg ggttcgcgaa tctcgatcag
     3001 gtctcgcaga tgttcggcgt cgatgtgatc gcgctcgtct cgatcgatca gtttcagtcg
     3061 agcgatcctt atccggtctc ctttctctat ctctccatcg tcggcgcgta tgtgcttccg
     3121 ctcgatcaca acgacacgcg cacgctgatc gacgcggcgg tcttctatgt ccctgcgcgg
     3181 cgattccttc tgcgggctcc cggacagagc cggatcaccg gacactcgac ggcggtggac
     3241 gtcagcgagt cgatgcggga gcgctcggtg aaaggtctgt cgatggcgat gaccgacctc
     3301 tcggcgaacc tctccagcga gaccgatcgg ttcaagcagg acatcatcag cggcgagcgc
     3361 aacgacgtcg acgtcgtcaa taaacaggga acgtcgatcc ggacgagcgg aagcatcggc
     3421 ggtctctggg cgctgtcgct cctgctcgtt gtggcgatgc ggagattgtg gtcatgaacg
     3481 cggaactgtt tgcgctgacg ccggccgcgg cgcacggcga gatctggcgc ctctggaccg
     3541 gacatctcgt tcattacgac ttccggcact tcctgaccaa tgcaatcgcg atcgctgtcc
     3601 cgatcgcact catcgatcgt cgcagccgcc gttcgatcct cctgtcgatg acgctcctcg
     3661 cgccgatgat cagcgtggcc ctgctcgcag gtgcgcattt cgacgagtac cgcggcgcat
     3721 cggccctcgc tcttgccgtt tgggtggcgt cggcacttac tcttctgcgc gggcaatcgg
     3781 cgagcgaccg ccacaccagc gccgccctga tcgctctcgc aatcggcaag ctggccttag
     3841 aaacggcagg cgccggccat ctctggacgg aggtcgcacc actaccgctc gcgcatgctg
     3901 ccggcgtgat cgcaggcgta gccgccttca tcgcagttac actgattccc acaccccact
     3961 cccaacaccc caccccccga cacgcggtcc aacaatcatg accaaatcaa ctcacacgcg
     4021 tctcggcgtt cccacccgca cggcagagga ttgggaggaa gcggcgctcg acgtcatcgc
     4081 cgcgcgcggt atcgccgcgg tttcgattcc ggaactggcg cgcacgctcg acgtcaccaa
     4141 gggcagcttc tactggcatt tcgccggcct tgacgacctt gtgagccgcg cggtcgcacg
     4201 atgggaccgg ctcgataaag agacgctcga tcagatccgg acgatcgagg attcggtcgt
     4261 gcgtctgcgg accctgtttg ccgaagccat gtctgcgcag agagcgcaat cgctttacct
     4321 ggctctggct gtctcgccgt cgccggatat cggtgcggca ttgcgaaagg tgtccagcag
     4381 gagactgaaa ctgctcgcgg ccacgtatca ggaacttgga atgacggaac ctgccgccca
     4441 acagcaggca ctgctcgcgt acagcgcgta catcggtggc gtgcacttgc gcaacaatga
     4501 cgcgccctgg ctccggcaaa cgcgagacgt gcaggcgtat gtcgagcacg cgtcgacggc
     4561 gctcatcgaa ccggcgagga aacgcaagac gaaggggtga gttgggtcac cccaaaacca
     4621 tgcaccacag agacactgag gtcacagagt tgagacgtta actctgtgcc ctctgaggct
     4681 ttgtggtgat ggttttaatc ttcttcccta tactcggcgg ccacaggaga cgttccatgc
     4741 acgtgattcg tctaattcgc gttagccttc tcgccgccac agcactgacg atgtccaaca
     4801 tagcgtttgc agagcccgcg atgaccgtac cgacgccgcc ggcgacggtg aagaaacctc
     4861 acgtcacgaa gatccatggc gacacgctgg tcgacaacta cttctggttg cgcgagaaga
     4921 gcaacccgga cgtcatcgcg cacctggagg cggagaacgc ctacaccgcg gcgatgacgg
     4981 cggacacgca gccgctgcag cagaagctct acggcgagat gctcggccac gtgaagcaga
     5041 ccgacgtcaa cgttccctat ctcctcggcg ggtattacta ctactcgcgc accgaggagg
     5101 ggaagcagta cccgatttat gcgaggaaga aggggagcct cgatgcagcc gaggagatca
     5161 cgcttgatct caacgaactg gcgaaaggcc tgaagtttct gtcgctcggc gcctatcggg
     5221 tcagcgatga cggaaacctc ctcgcctact ccaccgacac caccggctat cgccagtaca
     5281 cccttgtcat caaagatctc cgcacgggaa cgctccttcc cgagaagatc gagcgcgtcg
     5341 acacggtcat gtgggcgacg gataacaaga cgatcttcta tgtgaccgaa gacgccgtca
     5401 ccaagcgctc cgacagcttc taccgccacg tcctcggcac gccggcaaac gatctggtct
     5461 tccacgagcc cgacgagctt tacgacatcg gcgcgttccg aacgcgcgac aaggccttca
     5521 tcgtcgtcga gtcgcagagc aagacgacat cggaagctcg ctaccttccc gcgaataaac
     5581 cggccgaggc gctgcgggtc gtcgttccgc gcacgagcga ccacaagtac ttcgtcgacc
     5641 atcgcggcga cctcttctac atccgcacca acgatcacgc gaagaactac cggctggtga
     5701 ccgcgccgga ctccgctccg gaaatgaaga actggaaaga gatcatccca gcgcgggccg
     5761 atgtgaagct cgacgatttc gatctcttca aaaaccacat ggcggtgtcg gagctgaagg
     5821 gcggctcgca gaccatccgc acggtcgatt tgaagagcgg ccaatcgacg ccgatcgcgt
     5881 ttccggagcc ggtgtactcc gtctttctcg acaccaaccc ggagttcgac acggcgacgc
     5941 tgcgctatcg ctatcagtcg ctcgtgacgc caagctcggt cttcgactac gacatgggaa
     6001 cgcatgccag cacgctgctg aagcgtttcg aggttcccaa ctacgacgcc tcgcaattcg
     6061 cctccgagcg catctacgcc acggccgcgg acgggacgaa ggttccggtc tcgctcgtct
     6121 acaagaaacc tctcgttcgc gacggaaagc ggccgatgct gctttacgcg tatggatcgt
     6181 acggttcgcc aacgtggccg acgttcaact ccaaccggct gacccttctc gatcgcggcg
     6241 tgatctacgc catcgcccac atccgcggcg gcggcgagct tggcgaaccg tggcgcgagg
     6301 caggcaggat gatggtgaag cgcaacacct tcaccgactt caccgacgtc gtcgagaacc
     6361 tcgtcaagag caaatacacc tccagcgacc ggctcgtcat tcagggcggc agcgccggcg
     6421 gactgctgat gggcgccgtc gtgaacatgc gcccgcagtt gttcaaggca gtcgtggcgc
     6481 aagtaccgtt cgtcgatgtc atcaacacga tgctcgacgc cagcctccct ctgacaacga
     6541 gcgaatacct cgagtggggc aatccgaaca tcaagaagga gtacgactac atcaagacct
     6601 actcccccta cgacaacatc gccgcgaagg cgtatccggc gatgctggtg aaagtctcac
     6661 tgaacgacag ccaggtgccg tattgggagg gaacgaagtt cgtggcgaag ctccgcgaga
     6721 tgaagaccga ctcgaacccg ctgctcctca aggtgaacat gggagcgggt cacggaggat
     6781 cgtccggacg ctatgacgct ctgcgcgaaa cggccgccga tcaggcgttc atgctgaaag
     6841 cgatggggat cagcgagtag gagattctct ttgtgaaacc tatcgttcta gtcatgatcg
     6901 tacttgccgc cactggcgga gcctatgggg cagagttcaa catcacaaac tcgaatgaca
     6961 gcggcacggg ctcgttgcgc tgggcgatcg aacaggcgaa tgtctccccg gacggcggtt
     7021 acatctacgg taccggcaat gtcgtccatc tgacatcgcc actacctgcg atcactggcg
     7081 gcgtctacct ctcacatgtc acgttggaag gcggcggagc agggccaggc gacggactcg
     7141 tcctcgacag cgagtacgat tctatctacg acgttcatgt gaacaatttc aaaggcgacg
     7201 gcattgtgat ccgaggcgtc gccgacctgg cgctcgtgag cgcaaccgga aaccaaaacg
     7261 ggatccgcat cgaaggccgg tattcgttca tcgaggcaac gaccgcgacc ttgaacagcg
     7321 caaatggcat ctggatcaca tccaccggta gcgggaaccg gcttggcggg caggacccgg
     7381 atttttgttt tggagaaggg gacggttgtt tcattcccat cggagctggc gtttgggtcg
     7441 gtggcaacgg cggctcgggt gtccggatcg acggcgatgg aaacacgctc aacaacgcgc
     7501 acattggccg ggacgcaccg ctaaacctcg gattgccgag ccggccgctc gcgaacggtg
     7561 gcgatggaat tgtcgtgatc ggcgcacaca acaccgtgat gagctccacg gtgtcaaaca
     7621 gccgcggtta cggtctgctg ctcctcgcgc tggcaaatct cgaggacaat gctggatcct
     7681 gcaacgctcg cggatttgtg agtggcccgc tgatcgatcc cccgcgaatc actttcgcgc
     7741 gcgccgaccc cacagcaatc acgttctgcg ttctttttca gggcacgccg aacgcgcgtt
     7801 accgcatcgg actcgctgcc gcaacgacag catgtcctga cttcggcctg ccatcactcg
     7861 gcacaaccac ggtcaccaca gacgcaactg ggtatgcctc ctggacggca acgctcggca
     7921 cgggtttcac cgtgcctggt gtttctgcga ttgccactcg cggcgaagct gaggtttcgc
     7981 gccaatcgga catcgtggcg gcctttgcga ccagagagcc gcgtgccgat ctaaccgtgc
     8041 aaacgactgc accagggggt gcctcggcca ccgatctcat cgatttcgtt acgactgtca
     8101 cgaacattgg gccggccgcc gtcgacggat tcatcgtcga cattccacgc acaccaggca
     8161 ctgcctttct ctccgctgcg accacttccg gcgcctgtta cctcggcggt ctgcaggagt
     8221 gctttatcga gacactcgcc gtcggtgagt ccgcaacgat ccgcgagcgg gtgcgcgtcc
     8281 tggccaccag tgggaccctg caccacctcg caaccgtcag gcactacgac agcactagga
     8341 gccaaccaag agtagatccg aatcccgcaa acaacacttc taccgtagat gtccggatca
     8401 tcacgccacg gcaccacgcc acgggtcgct agcctggaac gaacgtcggg ggatggctga
     8461 agcgccgcca cgcagatgac gaccgactcg aacccgcccc tttgtacgca tcacgacatc
     8521 agctcatcga gcatcttctt tcgatggttc aggaagtccc tcgtgacgag gtagtggtca
     8581 gtctcctcgt acgctacttc gcgcattccg tcgtccagca gaaggattgt tgcatcggga
     8641 taggccatta cgatcggcga gtgcgtggcg atcaggaact gtgagccatg tttgacgagc
     8701 tcgtgaacgc gggctagtag cgcgagctgg cgagcaggag agagcgcggc ttcgggctcg
     8761 tcgaggatat aaagcccatc gccaagaaag cgattcacca gcaacgccag aaacgcttcg
     8821 ccgtgagact gctcatgcag cgacacgcct ccgtagcccc tggttacgcc gagccgatcg
     8881 atctccgtcg ccacgttgaa gaaggattcg gcacgcagga aatagccatc ggagggatag
     8941 cgtgccgagc gagtgatgtg gaagaacagc ggtcgttcgc cctcctcgtc gcggcgcacg
     9001 gtctcgaacc ggaagttcct cgagccgcct tcttcattga accccgccgc gacagcgagc
     9061 gcttcgatga tcgttgactt gccggacccg ttctcgccga cgaagaacgt caccggcttt
     9121 cgaaactcaa tgccctcgaa gcctcgcatc gcgggaacat cgagccggta gtcgcgcggc
     9181 gcgcgtggcc caaggtcagc gcgaatcacg aagagtttcg aatcgagagc ccgcaagatc
     9241 aaatcgtaac tgatcggcgg cggtcccaaa aacctgcaag tgagtcaact tgaaaagccg
     9301 gtcaccacgg agacacagag gtcacggaga tcccctctgt gttctctgtg tctctgtggt
     9361 tgcctttgct tcggcagcac ttccgtgtcg gaaaatcagc gcccgaggcg gctcaacgcg
     9421 gcggcaccaa gcgcgatcgt gagcaggagc aatgccgtgt gcgaaaacag cgggatctgc
     9481 gattgcggta cgacgaccag cgtggcgctg ccgccgcc
//
LOCUS       DATLVW010000730         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_279918, whole genome shotgun sequence.
ACCESSION   DATLVW010000730 DATLVW010000000
VERSION     DATLVW010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279918"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1780)
                     /locus_tag="VNN25_21225"
     CDS             complement(233..1780)
                     /locus_tag="VNN25_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94111.1"
                     /translation="MKFAPAVLGLSLLCSCTFTHTYQLRDTQPPIAAITLEQLEKRAD
                     LRDEKGNNLGCADPVFRKQSIEYFKNARGGQFSVGTIELSDDGHVKDDDQEAQVLADI
                     DRVANGSHGLILVTFVHGWHHRPKVCDNNIACFRRVLYALSISEQRPVYGVYIGWRGD
                     SIKGSTPRTSFLSALSFYERKNTAHLIGHEGGRELLQKLNEHYKNLNDKFRTADVPHP
                     VTMVTVGHSFGGALVYSAVEATLVRELKRREVNQSGKVALETDHVVGRPAEDGQPLPI
                     RPGIGDLVVLLNPAFEARRYTIFAEDLKNGKSHYRNDQLPVLLTVASEGDNAVGMAFP
                     AGRTLYFLLHPWRYRGMEDVIGQGHYDPQTTHNLVLTDAAGTQIYPKPEAKVTTYDAS
                     PEDIKRCDLHPDDGDFSTCECEYPVPTTLEEALGNNMTFVSQGRGAVKTGPNEKVALQ
                     VRNGVQWDEHSPFLVARVSASIVPAHSDIYTPRFVTFLAGYIHEFLKEAETHKPSSNV
                     AAASTSP"
     gene            complement(1910..3481)
                     /locus_tag="VNN25_21230"
     CDS             complement(1910..3481)
                     /locus_tag="VNN25_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94112.1"
                     /translation="MKARAILKRIVPLTILLTMTALSCGKEEPVPATTSSTTSSSATE
                     KAVAKPQVAVGSTAWQVPVDVTPTPPPNPPQANYLDLGWQTFVALSWPALSPTSGGVN
                     GQPDTTSTIGATAANGAFVPTVWSTYRDVATIMLNNGTDPGASYTQAVTIPSGCTPIG
                     SNPVAPGFQPMFIDGSSFPNATIVRDYINQATDNPLVDQKGWYTLTDMRIDQSEYNYI
                     HQNAYYLGANQAAAYSSKGQLQPFPRTGQEPTLPPNPPYAQYGALEVKAAWRVLDPTS
                     DKAVIPRYYTQWGYFMQPDGKTCQGPALFGLIGLHILRLTPSTGGTWFWASFEQVDNT
                     MPPSGIPATLAAPGTPNGNCTSAYNVGPPGVTGNIPWNNTNKPNNLCQVTPIPSDVQQ
                     ANTTWQGKLAGTVWQYYQMVNTLNPCPNGASGCATFPPIYDTTNQVNTPIFANTAIES
                     YFQDHTCMDCHGGADGDGAPTKMTATNQIFTFVLQNAYYPPTSAAATAAHTSFKNLFK
                     NPPHSKLVGAPAAKK"
     gene            complement(3526..>4173)
                     /locus_tag="VNN25_21235"
     CDS             complement(3526..>4173)
                     /locus_tag="VNN25_21235"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH94113.1"
                     /translation="AWLAELFPRATFVGADLEEPHLERARTRCAVFGDRVRFEIADAM
                     ALRYPDASFDFVVCRHVLQAVPDAAKVLAEIARVLRPGGRVHLIAEDYGMLLCHPTNL
                     DSDGFWQRFPPRMSAGFGSDLHVGRKTFTLLHDLGMTNIAADYIVVDTLRVDREVFAR
                     IWEAWRDGYTDALVEHSGASRDEIDQRWREMIDCVRDPRGYALWQVPVWTAAKAG"
BASE COUNT          769 a         1205 c         1355 g          844 t
ORIGIN      
        1 gctctcaccg gtgcggcggt ggcccttctc tacgcgccga tgaccgggaa acgaatgcag
       61 aggaaggtag ccgacgtgac cgagaaggta cgagacgtgg tcgaagactc tgtcgacaac
      121 gtgcagaaca tccttcgcaa agtcgcgaac gcgtaagcgc agagtcggaa tttgtgccgg
      181 atcgcggtgc ctcggaatgg ttcagccgag gcaccgcagt tgccggaaca cgtcacggtg
      241 acgtcgacgc tgcggccacg ttggatgacg gcttatgcgt ctcggcctct ttgaggaact
      301 cgtggatgta gccggccagg aacgtgacaa agcgcggtgt atagatgtcg ctgtgcgcgg
      361 ggacgatgga cgctgatacg cgcgcgacga gaaacggtga atgctcatcc cactggacgc
      421 cgttgcgcac ctgcagagcg accttttcgt ttggaccagt cttcacagcg cctcggccct
      481 gcgaaacgaa cgtcatgttg ttgccgagtg cttcctcgag ggtcgtcggg accgggtact
      541 cacactcaca cgtgctgaag tcgccgtcgt ccgggtgcag gtcgcagcgc ttgatgtctt
      601 ccggcgacgc gtcgtaggtc gtcaccttgg cttccggctt cgggtagatc tgagtcccgg
      661 cggcgtccgt cagcaccagg ttgtgcgtcg tctgcggatc gtagtggccc tgtccgatca
      721 cgtcttccat gccgcgatac ctccagggat ggaggaggaa gtagagcgta cggccggcag
      781 gaaacgccat acccacagcg ttgtcgccct ccgaagccac cgtcaggagc accggaagct
      841 gatcgttccg gtaatggctt ttcccattct tcaagtcctc ggcaaagatg gtgtaccgcc
      901 gcgcctcgaa cgcgggattc agcagtacga cgagatctcc gataccgggc cggatcggca
      961 gaggctggcc atcttcagcc ggccggccga cgacgtggtc ggtctccaac gcgaccttgc
     1021 ctgactggtt cacctcgcgt ctcttcagct cccggacgag cgtcgcttcg acggcggagt
     1081 aaacgagcgc gccgccgaaa ctatggccca ccgtcaccat cgtcaccggg tgcggcacat
     1141 ccgcggtacg gaacttgtcg ttgagattct tgtagtgctc gttcagtttc tggagcagtt
     1201 ctcgcccgcc ctcatgtccg atgaggtgcg ccgtgttctt tcgctcgtag aacgaaagtg
     1261 ccgaaagaaa cgaggtccgt ggagtactgc ccttgatcga atcgccccgc cagccgatgt
     1321 agacgccgta aaccggccgt tgctcggaaa tgctgagtgc gtagagcact cgccgaaagc
     1381 aggcgatgtt gttgtcgcag accttcggcc ggtgatgcca gccgtgcacg aaggtgacga
     1441 gaatcaggcc gtggctgccg tttgccactc gatcgatgtc ggccagaacc tgcgcctcct
     1501 gatcgtcatc cttgacgtga ccatcgtcgc tgagctcgat cgttcccacc gaaaactggc
     1561 cgccgcgggc gttcttgaag tattcgatcg actgtttccg aaacaccggg tcggcacaac
     1621 cgaggttatt gcccttttcg tcgcgcaggt cggcacgctt ttccagttgt tcgagcgtga
     1681 tcgcggcgat cggcggttgc gtatcgcgca actggtaggt gtgcgtgaag gtacacgagc
     1741 agaggagaga cagcccaagc accgcaggtg cgaatttcat gactcatctc cttttgtatg
     1801 tgagagacga atcgtatcgc aaactcacct tgaattcatc cggcaccttg acgatctgag
     1861 aagagcaagc aaggcacggg gagcaccctc cccgtgcctc acgagagact tacttcttcg
     1921 cggcgggagc cccgaccaat ttgctgtggg gcgggttctt gaacaggttc ttgaaggacg
     1981 tgtgcgcagc ggtagccgct gctgatgtcg gcggatagta ggcgttttgg agaacgaacg
     2041 tgaagatctg gtttgttgct gtcattttgg tgggagcgcc atccccgtct gcaccaccgt
     2101 ggcaatccat acacgtgtgg tcctggaaat agctctcgat cgccgtgttg gcgaagatcg
     2161 gtgtattcac ctgattggtt gtgtcgtaga tcggcgggaa ggtcgcacag cccgacgcgc
     2221 cgttcggaca cggattcagc gtgttcacca tctggtagta ctgccagacc gtcccggcca
     2281 gcttgccctg ccatgtggtg ttggcctgtt gcacatccga tggaatcggc gtgacctggc
     2341 agagattgtt gggcttgttc gtgttgttcc agggaatgtt gccggtgacg cccggggggc
     2401 cgacgttgta cgcgctcgtg cagttgccgt tgggcgtgcc tggagccgcg agcgtggccg
     2461 ggattcccga cggcggcatg gtgttatcga cctgttcaaa gctggcccag aaccacgtac
     2521 cgccggtcga tggcgtcaga cgcagaatgt gcaatccgat cagtccgaac agcgcaggcc
     2581 cctgacaggt ctttccgtca ggctgcatga agtaacccca ttgcgtgtag taccgcggaa
     2641 tcacagcctt gtcgctggtt ggatccagca cgcgccacgc cgccttcacc tcgagcgcgc
     2701 cgtactgcgc atacggcgga ttcggtggaa gcgtgggttc ctgaccggtc cgcgggaatg
     2761 gctgcagttg acctttgctg gagtaggctg ccgcctgatt ggcaccgagg tagtaggcgt
     2821 tctggtgaat gtagttgtat tccgactgat cgatcctcat gtcggtgagg gtgtaccaac
     2881 ccttctgatc gacaagcgga ttgtcggtcg cctgattgat gtagtcgcgg acgatggtgg
     2941 cattcggaaa cgatgatcca tcgatgaaca tcggctggaa gccgggcgcc acgggattgc
     3001 tcccgatcgg cgtacagccg ctgggaatcg tgacggcctg ggtatacgaa gctccgggat
     3061 cggtgccatt gttcaacatg atcgtggcga catcgcgata ggtgctccag acagtcggaa
     3121 cgaacgcgcc gttggcggcg gtggcgccga tcgtcgaagt cgtgtcgggc tggccgttga
     3181 cgccgcccga cgtgggagag agagcgggcc aactgagcgc gacgaaggtt tgccagccga
     3241 ggtcgagata gttggcctgg ggaggattgg gcggcggagt cggagtgacg tcgacgggaa
     3301 cctgccacgc ggtcgatccg accgccacct gcggcttggc gacagctttc tcagtggccg
     3361 aggatgatgt cgtcgaagac gttgtggcag ggaccggctc ttccttgccg caactcaggg
     3421 cggtcatcgt gagcaggatg gtcaagggga cgattcgctt gagaatggca cgtgctttca
     3481 ttcgattcct cctaactcta ggagtgtctc atggcgggct cttccctacc ccgcttttgc
     3541 ggccgtccac accggaacct gccacagggc gtacccgcgg gggtcacgca cacagtcgat
     3601 catctcgcgc cagcgctggt cgatctcatc gcgcgatgcg cccgaatgct cgaccagcgc
     3661 atcggtatag ccgtcgcgcc atgcttccca gatgcgggca aagacttcgc ggtcgacccg
     3721 aagcgtatcg accacgatgt agtcggcggc gatgttggtc atgccgaggt cgtggaggag
     3781 tgtgaaggtc ttacggccga cgtgcagatc gctgccgaag ccggcactca ttcgcggcgg
     3841 aaagcgttgc cagaagccat cggaatcgag gttcgtcgga tggcagagaa gcatgccgta
     3901 gtcttcggca atgaggtgaa cacggccgcc ggggcgcagc acgcgcgcga tctctgcgag
     3961 tactttggca gcatcgggga ccgcttgaag gacgtgccgg cagacgacga agtcgaagct
     4021 cgcgtcggga taccgcaacg ccatcgcatc cgcgatctcg aagcggacgc ggtctccgaa
     4081 cacggcgcaa cgagtcctgg cccgctccag gtgcggctcc tcgagatcgg cgccgacgaa
     4141 tgtagcgcgc ggaaagagct ccgcgagcca cgc
//
LOCUS       DATLVW010000731         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_609159, whole genome shotgun sequence.
ACCESSION   DATLVW010000731 DATLVW010000000
VERSION     DATLVW010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609159"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VNN25_21240"
     CDS             <1..534
                     /locus_tag="VNN25_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94114.1"
                     /translation="RESRIANAGACGVPKGYPGNGADPANPHFYNVYRFANPSGAAVC
                     FNFTLTYDSGSVDGSTGILRYLTAYSTYDPVNIGNNYLGDVGAVLTSPQSMGVTVPAG
                     SAIDVVVFAIDVAPNGAGSYTLNCSTGNADAGTGGADGGGGNDGGAPDGGAGGASGAD
                     GGGTDGNDGAADAAPDA"
     gene            complement(626..2038)
                     /gene="glnA"
                     /locus_tag="VNN25_21245"
     CDS             complement(626..2038)
                     /gene="glnA"
                     /locus_tag="VNN25_21245"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXH94115.1"
                     /translation="MTAAEALKLAEKNGAKMVDFKFVDLLGIWQHTTMPIGKLNADVF
                     KDGYFFDGSSIRGWQPIHASDMIFLPDPVSAVMDPFYALPTLSLTCDIVDAVTKQPYS
                     KDPRTIAAKAEKYLKSTGIGDTSYFGPEPEFFIFDEVRYSEGTNRASYFVDSVEGVWN
                     TDREEGPNLGYKPAKKGGYFPVPPTDTLTDIRAEMAMTLEAVGIPVEVFHHEVATGGQ
                     CELSMRFNSLKAMADWTMWYKYVVKNVARKHGRTATFMPKPLFGDNGSGMHCHQSIWK
                     DEKNLFAGDGYAGLSETALHYIGGILKHAPAICAFTNPGTNSYKRLVPGYEAPVNLAY
                     SSRNRSASVRIPIGGNSPKAKRLEFRCPDPTANPYLAFAVMMMAGLDGIQNKIDPGKP
                     LDKDIYALAPEEAKDIPKTPGALDQALNALERDHAFLLKGDVFTPDVISTWIDYKREN
                     EVDAIRLRPHPYEFALYFDN"
     gene            complement(2097..>2260)
                     /locus_tag="VNN25_21250"
     CDS             complement(2097..>2260)
                     /locus_tag="VNN25_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXH94116.1"
                     /translation="RVEIVVKDDIVRDVIEAILAVARTGRIGDGKIFVTPVEEAIRIR
                     TGERGEDAL"
BASE COUNT          366 a          722 c          748 g          424 t
ORIGIN      
        1 cgagaatcgc gtattgcgaa cgcgggcgcc tgcggagtgc ccaagggata cccaggaaat
       61 ggagccgatc cggcgaaccc gcacttctac aatgtgtatc gcttcgcgaa tccgagcggg
      121 gcggccgtgt gcttcaactt cacgctgacg tacgacagcg ggtcggttga cggatcgacc
      181 gggattctgc ggtacctcac cgcttattcc acttacgatc cggtgaacat cggcaacaac
      241 tatctcggcg acgtcggtgc ggtgctcacg tcgcctcagt ccatgggcgt caccgtgccg
      301 gcgggatcgg cgatcgacgt cgtggtgttc gcgatcgatg tcgcgcccaa cggggcaggg
      361 tcgtatacgt tgaattgctc tactggcaat gccgacgccg gaactggcgg cgcggatggc
      421 ggaggcggca atgacggcgg cgcaccggac ggcggcgccg gcggagcgag cggggccgac
      481 ggcggcggca cggatggcaa cgacggcgct gcggacgcgg cgccggacgc gtagcctcgt
      541 tccggccggc gggcgttggc tctcgcaagg tttgccctct ccctaaccct ctccccgcgg
      601 acggggagag gggatcggaa acggatcagt tgtcgaagta gagcgcgaac tcgtacgggt
      661 gcgggcgcag gcggatggcg tcgacctcgt tctcccgctt gtagtcgatc caggtcgaga
      721 tgacgtcggg cgtgaacacg tcgcccttga gcaagaacgc atggtcgcgc tccagcgcgt
      781 tgagcgcctg gtccagcgcg cccggcgtct tcgggatgtc cttcgcctct tccggagcca
      841 gcgcgtagat gtccttgtcc agcggcttgc ccgggtcgat cttgttctgg atgccgtcga
      901 ggccggccat catcatgacc gcgaacgcca ggtacgggtt cgccgtcggg tcggggcagc
      961 ggaactcgag ccgcttcgcc ttcggcgagt tgccgccgat cgggatacgc accgacgccg
     1021 agcggttgcg cgacgagtac gccaggttca ccggcgcctc gtagcccggc accaaccgct
     1081 tgtagctgtt cgtgcccggg ttcgtgaacg cgcagatcgc cggcgcgtgc ttcaggatgc
     1141 cgccgatgta atggagcgcg gtctccgaga gccccgcgta gccgtcgccc gcgaacaggt
     1201 tcttctcgtc cttccagatc gactggtggc agtgcatgcc cgagccgttg tcaccgaaca
     1261 gcggcttcgg catgaacgtc gccgtgcgcc cgtgcttgcg cgcgacgttc ttgaccacgt
     1321 acttgtacca catggtccag tcggccatcg ccttgaggct gttgaagcgc atcgacagct
     1381 cgcactgacc accggtcgcc acctcgtggt ggaacacctc gaccgggatg ccgaccgcct
     1441 cgagggtcat cgccatctcg gcgcggatgt cggtcagcgt gtcggtcggc ggcaccggga
     1501 agtagccgcc cttcttggcc ggcttgtagc ccaggttcgg gccctcttca cggtccgtgt
     1561 tccacacgcc ctcgaccgag tcgacgaagt agctggcgcg gttggtcccc tcggagtagc
     1621 ggacctcgtc gaagatgaag aactcgggct cggggccgaa gtacgacgtg tcgccgatgc
     1681 cggtcgactt gagatacttc tcggccttgg cagcgatggt gcgcgggtcc ttgctgtacg
     1741 gctgcttggt gaccgcgtcg acgatgtcac aggtcagcga cagcgtcggc agcgcgtaga
     1801 acggatccat caccgccgac accgggtcgg gaaggaagat catgtcggag gcatggatcg
     1861 gctgccaacc gcggatcgag ctcccgtcga aaaagtagcc gtccttgaac acgtcggcgt
     1921 tcagcttccc gatcggcatc gtcgtgtgct gccagatgcc aagcaggtcg acgaacttga
     1981 agtcgaccat cttcgcgccg ttcttctcgg ctaacttcag tgcctcagct gctgtcatgg
     2041 atctacccct tatgcctggt gagtgtgttg ggtgaacgaa acttactctc gagcggttac
     2101 agcgcgtcct cgccgcgctc gcccgtgcgg atacgaatcg cttcctcgac gggcgtcacg
     2161 aagatcttcc cgtcgccgat gcgcccggtg cgggccaccg ccaagatcgc ttcgatcacg
     2221 tcacgaacga tgtcgtcctt gaccacgatc tccacgcgaa
//
LOCUS       DATLVW010000732         5273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_566917, whole genome shotgun sequence.
ACCESSION   DATLVW010000732 DATLVW010000000
VERSION     DATLVW010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566917"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1796
                     /locus_tag="VNN25_21255"
     CDS             288..1796
                     /locus_tag="VNN25_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94117.1"
                     /translation="MTTSRKLFVSTIAITAVILSTMSARAEQPDKYTRPHEPFTAPGM
                     QVRMSIVNPINSPWQTRIQPTVFQEIAPCRLSSTLTLDRYDTPWGGTAYLPNEARVYR
                     SRGVLQTPMFVDPCSEAVPIDAIGIVGRFTVTPGDGDGEVHIDPIQPGSPNATTVFKF
                     KKGEILMFEAGVMLSPDGTFGVQTWNAGADVTIDVLGYLLADPNAAGEKGDKGDQGYQ
                     GVQGETGATGAKGDTGATGAIGPIGPIGPIGPKGDKGDTGAVGATGAKGDKGDKGDIG
                     AVGATGAKGDTGANGVKGDKGDTGANGATGAKGDKGDTGANGAKGDKGDTGATGAKGD
                     KGDTGADGVKGDKGDTGLQGPMGPMGPQGPKGDKGDTGATGNTGEKGDKGDTGAKGEK
                     GDRGDIGLQGPMGPMGPQGPKGDKGDKGDKGDPGVSVVKGNACYPPGNNANSELTVPD
                     SRVHVTSVVILNYTDAGSNGNALAIVSQGEGSFQTTGSPNRCFQYAIFNMVP"
     gene            2223..2780
                     /locus_tag="VNN25_21260"
     CDS             2223..2780
                     /locus_tag="VNN25_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94118.1"
                     /translation="MTAIASSQWPQQYHSACWRVDGLLAPAEGVMNPVRSFTSTLIGF
                     AAAVIVAAVTLAIRDAGSFHRGGEFASAWQSAEFDLLTWGIVLIFSGGAFALVTLFLI
                     PSGRVLPLPLIVGLVCGTAVVASFGFGFANHLQRSAGIPWPLIAMAPGVASGAATVAL
                     ALVTNLFSGSSPYHLDHDPNTDSID"
     gene            complement(3054..4199)
                     /locus_tag="VNN25_21265"
     CDS             complement(3054..4199)
                     /locus_tag="VNN25_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94119.1"
                     /translation="MRRGNIKRSNVWRSVVLAVALALTGISPELAASPSVARIANAWR
                     HIEPKAALVHTQPPARLPVAAASVTRKRAAAPPPLPACAAGVVAHLVEVGDLATDDKY
                     IYFVDGEDLIARVAKEGGTPTLLGEVPGALIVSMTTDDARIYFTTWDDDSLTGSIYSI
                     AKDGGPVRALVRGLATPFDLTVDGQFVYWLAVGTSAGDGFLADGAIGRAAKADGSGVL
                     KLASNLSLPVSLTLDATNVYYSETGAGLGNPSVGLRRVPLAGGPISKLVDGIIVGPVA
                     VDSATVYYGTLTGSGTFEIASLPKSGGNPRLLVSGLDDISSLKLFGSTLYYVSESDET
                     SINAVPAAGGARRMVRTGGLATAKIAIEECLLYFASDENSTLERIPR"
     gene            complement(4348..4650)
                     /locus_tag="VNN25_21270"
     CDS             complement(4348..4650)
                     /locus_tag="VNN25_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94120.1"
                     /translation="MKKTSAKPDPDPDLDDDLRPHYDFDFKTWKPNRFAGMDLKFKGR
                     PPIILDADVAEVFDSPEAVNTVLRSVIKAMRRSDVGVKPKKARSTASPAANKRRAS"
     gene            complement(4631..4927)
                     /locus_tag="VNN25_21275"
     CDS             complement(4631..4927)
                     /locus_tag="VNN25_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002800717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXH94121.1"
                     /translation="MADQRSRIFRWYDRKAETNRARHGVGFEEAESVFDDPLARTDPD
                     PDHSDDENRELIFGHSAKGRLLLVGFVERGDSVRIISAREADKEERKRYEEDIR"
     gene            complement(4946..>5273)
                     /locus_tag="VNN25_21280"
     CDS             complement(4946..>5273)
                     /locus_tag="VNN25_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH94122.1"
                     /translation="EEFFALLDRVTRGEPGLTPLLARKLLQEFAKPVAEPKVSEDMPD
                     ALTTREREVLELMVDGVTSNRKLAKRLDLSENTVKFHVRNILDKLHLHNRAQVVGYAL
                     RKKMIE"
BASE COUNT         1026 a         1591 c         1685 g          971 t
ORIGIN      
        1 gccgcgcagg attgcgtaat cgttctcgat caaagcgatc ttcacgatcg agccgaagtc
       61 ctcggcatcc gcaccgcgca gtccggctcg gcggcacaca ccggcaatga cgcgctccac
      121 aagcgcgaga ttcgcgcgaa acagctcctc agcagcattt tctatggagt cagaataccg
      181 gaaagtttcg ggaatcgtgg aaaaacactt ttggcagtcc cactccctct tcgaatcgca
      241 aagccggcag caagcgaaag agagatcgca gagaaagaga ggacaccatg actacatctc
      301 ggaagctttt cgtatccacg atcgcaatca ccgccgtaat cctgtccacg atgtctgccc
      361 gcgccgaaca gccggacaag tacacgaggc cgcacgagcc gttcacggcg cccggcatgc
      421 aggtacggat gtcaatcgtc aatcccatca acagtccgtg gcagacccgc atccagccga
      481 cggtgttcca ggaaatcgcg ccatgccggc tgtcgtcgac gctgactctc gaccgttatg
      541 acactccctg gggcgggaca gcgtatctcc cgaacgaagc acgtgtttat cggagccgcg
      601 gtgtgctcca gaccccgatg ttcgtcgatc cctgttcgga agcggttccg atcgacgcga
      661 tcggcatcgt cggacgtttt accgtaacgc ccggagacgg cgacggcgag gttcacatcg
      721 atccgatcca gccaggcagc ccgaacgcga cgaccgtatt caagttcaag aagggcgaga
      781 ttctgatgtt cgaagcgggc gtcatgctca gccctgacgg taccttcggc gtccagacgt
      841 ggaatgccgg cgcggatgtg acgatcgatg tcctcggcta cttgctggcc gacccgaatg
      901 ccgccggtga aaagggcgac aagggcgacc agggctatca aggcgttcaa ggcgaaaccg
      961 gtgccacggg cgcgaagggc gacactgggg cgactggcgc aatcggtcct atcggtccta
     1021 tcggtcctat cggtccaaag ggcgacaagg gtgataccgg tgcagtcggc gcgaccggcg
     1081 cgaagggcga caagggcgac aagggcgaca tcggagcggt cggcgcaacg ggcgcgaagg
     1141 gcgacaccgg agccaatggc gtaaagggcg acaaaggtga caccggagcc aatggcgcga
     1201 ccggggctaa gggcgacaag ggcgataccg gagccaacgg cgcaaagggc gacaaaggtg
     1261 acaccggtgc aaccggcgcg aagggcgaca agggtgacac cggagccgat ggcgtgaagg
     1321 gcgacaaggg cgacaccgga ctgcagggtc cgatgggtcc gatgggtccc caaggaccca
     1381 agggagacaa aggcgacacc ggcgctaccg gcaacactgg cgagaagggc gacaagggag
     1441 ataccggtgc gaagggcgag aagggcgaca ggggcgacat cggactgcag ggtccgatgg
     1501 gtccgatggg tccccaagga cccaagggag acaagggcga caagggcgac aagggcgatc
     1561 cgggcgtcag cgtagtcaaa ggaaacgcct gctacccgcc gggaaacaac gccaacagtg
     1621 agcttacggt tcccgacagc cgggtgcacg tgaccagcgt cgtgatcctc aattacaccg
     1681 acgccggctc caatggaaac gcgctggcga tcgtttccca gggcgaagga tcgttccaga
     1741 ccacgggcag cccgaaccgc tgcttccagt acgccatctt caacatggtt ccgtaacgag
     1801 agaagtgcaa agtgcacggc ccgcggcgtt catcgccgcg ggccgctttt ttgtttgcat
     1861 gtgtttctca gggttcagcg gcaatcgagc ttggtgtcat cctggtgtca tcctggtgtc
     1921 atcctgagac tgccggagcc gcccgcacgg atcgcgatgt ttgcgcagtg cgtgggcggc
     1981 ggcgtaggac gtcgaaggat ctcaaacttc gcagatcgtg ttcgtccagc gcgagcgatc
     2041 gtgttgaaag gcgcccgagc gagatcttcg cactttgaga tccttcgccg tcctgcggcg
     2101 cctaggatga tctacgcgaa agtaacgtgc tgcgcgggcg cctccggcag gctcaggatg
     2161 acacatcacg ttttgcgcgt accgtcacgg ttgcgaagaa cggtgcagaa gcacctatca
     2221 ggatgacagc aatcgcatca tcccaatggc cacagcagta ccattcggcg tgctggcgcg
     2281 tcgatggact gttggcgccg gcggagggcg tgatgaatcc tgtccggagc ttcacctcga
     2341 cgctcatcgg ttttgccgcc gccgtcatcg tcgccgcggt cactctcgcc attcgcgatg
     2401 caggatcgtt tcaccgcggc ggcgaattcg catccgcgtg gcagagcgcg gagttcgatc
     2461 tgctcacgtg gggcatcgtg ctcatcttca gcggcggcgc tttcgctctg gtgaccctct
     2521 tcttgattcc atcgggccgc gtgcttcctc ttccgctgat cgtcggtctc gtctgcggca
     2581 cggctgtggt cgcatcgttc gggttcggat tcgctaatca cctgcaacga tctgccggga
     2641 ttccgtggcc cttgatcgct atggctccgg gagtggcctc cggcgcagct accgtggcgc
     2701 tggcgttggt cacgaatctg ttttcgggca gctcgcctta tcacttggac catgatccga
     2761 atacagattc gattgactga cgagcaggca acgcgcctcc gcgaggttgc ttccgcccag
     2821 gaccgatcga tggcggacct catccggcag ggcgtcgaca ccttgctgga ttcttctccg
     2881 acgacacgcg atcgagctgc cctgcgcgag gcggcaatcg cgatggccgg ccggcaccga
     2941 tcgcggctcg gggatctagc aaaaagacat gatcactatc tggcgacgga cttgacgcgt
     3001 tgaccactgt gtggcacaga cactcttgtc tgtgctttgt cttccattgc cgctcagcgc
     3061 gggatgcgct ccagtgtcga attctcgtcg gatgcgaagt agaggaggca ttcctcgatc
     3121 gcgatcttgg cggtggcgag accgcccgtt ctcaccatgc gtcgcgcgcc gccggcggcc
     3181 ggaacggcgt tgatgctggt ctcatcactc tcgctgacgt agtagagcgt cgagccaaat
     3241 agtttgagac tcgatatgtc gtcgagtccg gagacgagca atcgaggatt gccgccgctc
     3301 ttcggtagac tggcaatctc gaacgttcca ctgccggtga gggtgccgta gtaaacggtt
     3361 gcgctgtcca cggctacggg accgacgatg atgccatcca ccagtttcga gattgggccg
     3421 cccgccagcg gcacgcggcg gagtccgacg ctcggattgc cgaggccggc gccggtctcg
     3481 ctgtagtaga cgttggtggc gtcgagggtg agcgaaaccg ggagggagag gttcgaagcc
     3541 agcttgagca caccggatcc gtccgccttc gccgcgcggc cgatggcgcc atcggcgagg
     3601 aaaccgtcgc cggcggacgt gccaaccgcg agccagtaga cgaactggcc atcgaccgtg
     3661 agatcgaacg gagtagccag accgcgcacg agagccctca ccggaccgcc atctttcgcg
     3721 atcgagtaga tcgagccggt gagcgagtcg tcgtcccagg tggtgaagta gatccgcgcg
     3781 tcatcggtcg tcatcgaaac gatcagcgcg ccgggaacct cgccgaggag agtcggagtc
     3841 cctccctctt tcgccacgcg ggcgatcaga tcctcaccat cgacgaaata gatgtacttg
     3901 tcgtccgtgg ccagatcgcc gacttccacg agatgggcaa cgactccggc cgcgcaagcc
     3961 ggaagcggag gcggggcggc cgctcgcttc cgcgtgacgg acgctgcggc gacaggcaag
     4021 cgtgcgggcg gctgcgtgtg cacgagtgcc gctttcggct cgatatgccg ccacgcgttc
     4081 gcgatgcgag ctaccgaggg cgatgccgca agctcgggcg agattccagt aagcgcaaga
     4141 gccaccgcca ggactacgga tcgccacaca ttcgaccgct tgatgtttcc tcgtcgcatt
     4201 ccgtgctcct tcgccggccc gtggcggcta tggcacgttc gaatgcaggt gtcgtggcgg
     4261 ccttacggaa agacagagca taccgcgcgg cgtgagctcc gagttgccga aacggtgaaa
     4321 ctcaggagcg cgtgttgagt ctgcgcgtca ggaagcgcgc cgcttgttcg cggccggcga
     4381 ggccgttgac ctggccttct ttggcttaac accgacgtcg gacctgcgca tcgccttgat
     4441 gacggatcgc aaaacggtat tgacggcctc gggggaatcg aagacctcgg cgacgtcggc
     4501 atcgagaatg atcggcggac gtcctttgaa cttcaggtcc atgcccgcga agcgattggg
     4561 cttccaagtc ttgaaatcga aatcgtaatg gggacgaagg tcgtcgtcga gatcgggatc
     4621 cggatcaggc ttagcggatg tcttcttcat agcgttttcg ttcttctttg tcggcttctc
     4681 gagcgctaat gatgcgaacc gaatcgccgc gttcaacaaa gcctaccaag agaagccttc
     4741 ccttggctga atgaccgaag atgagctcgc gattctcgtc gtcagaatga tcgggatcag
     4801 gatcggtccg agcgagcgga tcgtcgaata cagactctgc ttcctcgaag ccgactccgt
     4861 gtctggcacg gttcgtttct gccttcctgt cgtaccatcg aaaaatgcgt gagcgctgat
     4921 cggccatgcc ttaaagctcc gcacctcact cgatcatctt cttcctcagc gcatacccca
     4981 ctacttgcgc gcggttgtgg aggtgcagct tgtcgaggat gttgcgcacg tggaacttga
     5041 ccgtgttctc gctgaggtcg aggcgtttgg ccagcttgcg gttcgacgtc accccgtcca
     5101 ccatcagctc gagcacttcg cgctcccgcg tggtgagggc gtcgggcatg tcttccgaga
     5161 ccttcggctc agccacgggc ttggcgaact cctgcaggag cttgcgcgcc agcagcggtg
     5221 tgagtcccgg ctctcctcgg gtcacacgat cgagcagcgc gaagaactcc tcg
//
LOCUS       DATLVW010000733         4182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_157120, whole genome shotgun sequence.
ACCESSION   DATLVW010000733 DATLVW010000000
VERSION     DATLVW010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157120"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VNN25_21285"
     CDS             complement(<1..534)
                     /locus_tag="VNN25_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94123.1"
                     /translation="MTYYVEKQLAFGPIRFGVSTRRALDAIDDDLALSSGPNGEFVRR
                     RNDGFFFGDTERVVGPSLPVSRTINQTPFWTSLKPDGTPRSFGFLALMGFGVLLILLG
                     LSVLIRKGPQGWVEIILGAICIAVPIVMTAQQRREIHDREERERVEREAAVARNQQLL
                     TWYTKALEHLLIDRGDNA"
     gene            complement(582..938)
                     /locus_tag="VNN25_21290"
     CDS             complement(582..938)
                     /locus_tag="VNN25_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXH94124.1"
                     /translation="MKRILIVTLVLLGAACSRQESKPSTSGDPAKGKQLITQYGCTAC
                     HSIPGIEGPRGEVGPSLEHVAVRPLIAGKLPNNPQNLTQYLLNPQTVSTLNIMPNLGI
                     KPDEARDIAAYLYTLK"
     gene            1131..1856
                     /locus_tag="VNN25_21295"
     CDS             1131..1856
                     /locus_tag="VNN25_21295"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXH94125.1"
                     /translation="MSALYALAAFAMTLAGGAFAFRYRQYLLYIMAFSSGLLIGVAFL
                     DLVPEVFELAHLHTIDIRVLMLTLIMGFLAIFLLEKLTIIHSEKQHDEPGHHHNVGLV
                     GAIGLSFHSFLDGLAIGVGFKAGTQVGLVVLLAVVAHDFADGLNTVTFMLATRNSRWR
                     TGALLGIDAIAPVLGALCAGVFYIAPRVLAFQLSFFAGFLLYLGASDLLPQVHARTRG
                     TLIAVTIAGVVVAGLLVFALQPA"
     gene            1853..4144
                     /locus_tag="VNN25_21300"
     CDS             1853..4144
                     /locus_tag="VNN25_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH94126.1"
                     /translation="MKFPIRWSDRTALALLIALLVVAYADILFLGRGFYISDLSVYHY
                     PMKHVVREAVRSGEFPYWNRLYSGGAPLAANPAYELFYPPQWLVYVLPFHFGVQLHVL
                     LHFAIAMAGMFLLLRRLGLSIAAAAFGSIAFVFCGSYVSLSSKLPILFSISWMPLALW
                     LLMRFDEERTARRLLPLAFVLAMQLLLGEPAGVMQTWAIIGGWALLRVFRSRQKKAEA
                     KGIAATLGVAMATAILIAAVQLLPAIDFVRDTVRTKAFDFRVVSNWSTPPVRLVELIL
                     PGVLRHVAAASGNAFITTIYPFRNDAYLTEIYAGLLIVLLAMAGVLAGVRSRWLFLGT
                     FAASIALAMGDHTPLLRVLYDSGILASFRFPEKFLLTAAFAIAVWSAVVFDRLLDGDR
                     RLIRWAFGLSIVWLCVSIVLFLRSSDGGALGTAHYFVVNCVRAASIAVWLWLARTQRG
                     RAMAAAGVLLLAADLWYGTLNAVPRERREFFDPAPAVANIEASAPYRVFNEVAWMGWE
                     GDPIVNEMFNIAQGPAFSWVMRNSVFPELPALRGLAVAMEDDIDLTSLRNTDDFRQAF
                     KGARSSRLPGADEPYARMANVGWRVRQRPFDDALRQQILTDASKAVTVEVVPSTRAPR
                     YAFAGRLERTPDPSGFRYAVEANAADPSVAYIDADAFSPAPGRVIGFRETANTATIDV
                     VTSGRSFLVVSVTGHRYWSATIDGHPARLVATNLAFQGIEVPAGAHMINMRYRNPLVP
                     IGGVISILSLAVLSVLAWARRAD"
BASE COUNT          638 a         1299 c         1346 g          899 t
ORIGIN      
        1 tgcgttgtct ccgcggtcga tgagaaggtg ctccagcgct ttcgtatacc aggtgaggag
       61 ttgctgattg cgcgcaacgg cagcttcgcg ctccacgcgc tcgcgctctt cgcggtcgtg
      121 gatctcgcgc cgctgctgcg cggtcatcac gatcggaacg gcgatgcaga tcgcgccgag
      181 gatgatctcg acccatccct gcggaccttt tcgaatgaga acgctaaggc cgaggaggat
      241 caggagcacg ccgaagccca tgagcgccag gaaaccgaag ctgcgcggcg tgccgtcggg
      301 cttcagcgat gtccagaatg gcgtttggtt gatggtccgc gaaaccggaa gactcggccc
      361 gacgacgcgt tccgtgtcgc cgaagaagaa accgtcgttg cgccggcgaa cgaattcgcc
      421 gttcggtccg gagctgagcg ccagatcgtc gtcgatggcg tcgagcgcac gccgcgtgga
      481 gacgccgaag cggatggggc cgaacgcgag ttgcttctca acgtagtacg tcatcggtag
      541 ccgcctctgc tgtgtgcgga cttgatgcca gccaccgatt cctacttcag cgtgtacagg
      601 taagcggcga tgtcgcgggc ctcatcgggc ttgatgccga ggttgggcat gatgttcagc
      661 gtgctgacgg tctgcgggtt cagcagatac tgagtcaggt tctgcggatt gttcggcagc
      721 ttgcccgcga ttaatgggcg caccgccacg tgctcgagcg acggcccgac ctcgccgcgg
      781 ggtccctcga tgccggggat cgaatgacag gcagtgcagc cgtactgcgt gatgagctgt
      841 ttgccttttg cagggtcgcc ggaagtggag ggcttcgact cctgccggct gcaagcggct
      901 ccaagaagca caagggtaac gatcaagatt cttttcaaac ggtttccttt cgacgaaagc
      961 gtggtgccaa caataccgcg ggcttgagat gtggacctca tcgtcaaaga caaccacaga
     1021 gacgcagagg acacagagga gatctctgtg acctctgtgt ctcagtggtg acctgttttc
     1081 aatggcgcac gctcgaagct gattccttcc tcgcgataga ctcaccaacg atgagcgcgc
     1141 tctacgcact ggcggcattt gccatgactc tggctggcgg tgcgttcgcg ttccgctacc
     1201 ggcagtacct gctttacatc atggcgttct cgtcgggatt gctgatcggc gtcgcctttc
     1261 tcgatctggt ccccgaggtc tttgagctgg cgcatctgca cacgatcgat atccgcgtgc
     1321 tgatgctgac gctgatcatg ggcttcctgg ccatcttcct tctcgagaag ttgacgatca
     1381 tccacagcga gaaacagcac gacgagcccg gccatcatca caacgtcggg ctggtcggtg
     1441 cgatcggatt gtccttccac agcttcctcg acggactggc gatcggagtc ggattcaagg
     1501 ccgggacgca ggtcgggctg gtcgtgttgc ttgctgtcgt cgcgcacgat ttcgcggatg
     1561 gtttgaacac cgttacgttc atgctggcga cgcgcaacag ccgctggcgt accggcgcgc
     1621 tgctgggcat cgacgcgatc gcgccggtac tcggcgcgct ctgcgcgggg gtgttttaca
     1681 tcgcgccgcg cgtcctggcc tttcagctct cgttcttcgc cggcttcctc ctctacctcg
     1741 gcgcgagcga tctcctgccg caggtgcacg cgcgaacgcg cggcacgctc atcgccgtga
     1801 cgatcgccgg agtggtggtg gccgggcttc tcgttttcgc gctgcagccg gcatgaagtt
     1861 tccgatccga tggagtgacc gcaccgcgct cgcgcttctc atcgcgctgc tggtggttgc
     1921 ctatgcggac atcctcttcc tcgggcgcgg cttctacatc agtgatctga gcgtctatca
     1981 ctacccgatg aagcacgtgg tgcgtgaggc cgttcgcagc ggtgagtttc cttactggaa
     2041 ccggctctac tcgggaggag cgccgctggc ggccaaccct gcgtacgagc tcttctaccc
     2101 gccgcagtgg ctcgtctacg tgctgccgtt tcacttcggc gtgcaactgc atgtgctcct
     2161 gcacttcgcg attgccatgg ccggcatgtt tctgttgctg cggcgcctcg gcctgtcgat
     2221 cgccgccgca gcattcggga gcatcgcatt cgtcttttgc ggcagctacg tgtcgctatc
     2281 gtcgaagctc cccatcctgt tttcgatctc ctggatgccg ctcgcgctct ggctcctgat
     2341 gcgctttgac gaggagcgaa cggccaggcg tctgctgccg ctggcgttcg ttctggcgat
     2401 gcaactcctc ctcggcgagc cggcaggggt gatgcagacc tgggccatca tcggcggctg
     2461 ggcgctactg cgggtttttc ggagccggca gaagaaagcg gaggcgaaag ggattgccgc
     2521 cacgctcggt gtggccatgg ctacggcgat tctcatcgcc gcggtgcaac tcctgccggc
     2581 aatcgatttc gttcgcgata ccgtgcgtac caaagccttc gactttcgcg tcgtttccaa
     2641 ctggagcacg ccgcccgtgc ggctggtcga gctcattctg ccgggtgttc tccgccacgt
     2701 cgccgcagcg agcgggaatg cgttcatcac cacgatctat ccgttccgca acgatgccta
     2761 tctgacggag atctacgccg gtctcctgat cgtgctgctc gcgatggcgg gcgttctggc
     2821 gggagtgcgc agccgatggt tgttcctcgg aacgttcgcg gcgagcatcg ccctggcgat
     2881 gggcgatcac acgccgctgc tgcgcgtgct ctacgacagc ggcatcctgg cttcgttccg
     2941 ctttcccgag aagttcctcc tcaccgcggc gttcgcgatc gccgtctgga gcgccgtcgt
     3001 cttcgaccga ctgctggatg gagaccgccg gttgatccga tgggctttcg gtctgtccat
     3061 cgtctggctg tgcgtctcga tcgttctgtt cctccgttcc tcggatggcg gtgcgctcgg
     3121 cacagcacac tatttcgttg tcaactgcgt tcgcgcggcg tcgatcgccg tctggctttg
     3181 gcttgcccgg acccagcgag ggcgcgccat ggccgcggcc ggcgtgttgc tcctcgccgc
     3241 ggacctttgg tacggcaccc tcaatgcggt tccgcgcgag cggcgggagt tcttcgatcc
     3301 cgcgccggcc gtggcgaaca ttgaggcatc tgccccatac cgcgtgttca acgaagttgc
     3361 gtggatgggt tgggaaggcg atccgatcgt caatgagatg ttcaacatcg cacaggggcc
     3421 ggccttctcc tgggtcatgc gcaacagcgt gttcccggag cttccggcgc tacgcggcct
     3481 ggcggtggcg atggaagacg acatcgacct gacctcgctc agaaacacgg acgacttccg
     3541 gcaggcgttc aaaggtgcgc gatcgagccg tcttcccggt gccgacgagc cgtatgcgcg
     3601 aatggccaat gtgggctggc gggtgcggca acggcccttc gatgatgccc tgcggcagca
     3661 gattctgacc gacgccagca aggcggtgac agtcgaggtc gtgccgtcga cgcgtgcgcc
     3721 gcgctatgca ttcgcggggc ggctggagcg cacgccggac ccgagcgggt tccgctatgc
     3781 cgttgaggcc aatgcggccg atccgtccgt cgcctacatc gacgcggatg cgttttctcc
     3841 cgcgcccggc cgcgtgatcg gtttccgcga aacggcgaac actgccacca tcgacgtcgt
     3901 caccagcggc cgctcgtttc tcgtggtcag cgttaccggt catcgttact ggtcggccac
     3961 gatcgatggg cacccggcac ggctggtcgc cacgaatctg gcgtttcagg gaatcgaggt
     4021 tccggcaggt gcacacatga tcaacatgcg ttatcgcaat ccgcttgtac cgatcggcgg
     4081 cgtgatctcg attctctcgc tggcggtgtt gtctgtcttg gcgtgggctc ggcgtgctga
     4141 ttagaaccgt ttcacgcgga gacgcggagg acgcggcggg tt
//
LOCUS       DATLVW010000734         9020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_527713, whole genome shotgun sequence.
ACCESSION   DATLVW010000734 DATLVW010000000
VERSION     DATLVW010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527713"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VNN25_21305"
     CDS             <1..573
                     /locus_tag="VNN25_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94127.1"
                     /translation="AGMRNVRRNPLVIAVAVLFFFATCATAKTPPPPKLPEWKAVPAS
                     VLDSFCANFREEGISSSTTINVVKTSQKYLITPLSMQALSDFGFYHGSMDSAKATSAA
                     LASSDEIPVTMPNDCAWRPIEPKSSARYPDTMTLEISPPITNPFSRNATGLFARLTLG
                     GEASTWYWLPLIPRGEIWIAGKMALLPYRQ"
     gene            complement(602..1015)
                     /locus_tag="VNN25_21310"
     CDS             complement(602..1015)
                     /locus_tag="VNN25_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH94128.1"
                     /translation="MRSVFADANYWIAIVNPKDNLHAKARQASATLGRTRIVTSEMVL
                     AEVLNAFASKGEFLRTRASLLVDQIQSSPDAEILPMTSSAFREALGRYRRRSDKTWGF
                     TDCTSFLIMEQRGIKQALSADRDFQQAGFETLLAD"
     gene            complement(1012..1791)
                     /locus_tag="VNN25_21315"
     CDS             complement(1012..1791)
                     /locus_tag="VNN25_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94129.1"
                     /translation="MSDEIRDVSTITFSGPRFWDAGLEADALLELLAYRKLLVETAKE
                     LWRDANGGLRLPRGFQKSIRLKFYFIESESEAIALKRVVSRDNPHSGKSLGDFAEIDD
                     AALLIDETITAVSEWRPIPDRMPKRSLPLLAAFGKSLRQGESIETRAMRSATAAELTR
                     ETCRRLALLSGGSYEPRREAMTPEETTPRYFEIRDSAVAEIPAYEEETMNDTYDDAAK
                     PLWQSIIDVSASVPPEEWDRVPKDLATNLDHYLYGRSREDE"
     gene            complement(2102..2656)
                     /locus_tag="VNN25_21320"
     CDS             complement(2102..2656)
                     /locus_tag="VNN25_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94130.1"
                     /translation="MQTTENVHSVGAGTPDPEALDAVVQEAVAHVQAIYQILSDFVPA
                     PVEGFREVRTACTVPDEFLEASAVAAENDESMQAATGLDPARTRMVINRNLRFEPLAA
                     AAEALARDIRYNILRERWEVVKQALQMFSLAKGYTRNNRSAALVAHVIAMQSALGRTG
                     RPRRKKADSVSHGAGVLKQSGEKE"
     gene            complement(2826..2987)
                     /locus_tag="VNN25_21325"
     CDS             complement(2826..2987)
                     /locus_tag="VNN25_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94131.1"
                     /translation="MHAFRFPVDAFRFRIHALRLRIHAFQLRIHAFWLRIHAFWFRVD
                     AFRYRIHAL"
     gene            3036..3458
                     /locus_tag="VNN25_21330"
     CDS             3036..3458
                     /locus_tag="VNN25_21330"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXH94132.1"
                     /translation="MLPVIVRSAASLRNDIEAGPHRMIADEPLAAGGTELGPTPYDFL
                     AAALGTCTSMTMRVVAKKEDIPLEGVEITIENDRMYAKDCADCLSTSGFIHRFTVAIK
                     LLGNLTPAQRGRMLDVAGRCPVNKTLRSEIRIDERLVD"
     gene            3505..3978
                     /locus_tag="VNN25_21335"
     CDS             3505..3978
                     /locus_tag="VNN25_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94133.1"
                     /translation="MTPPAVETRQRVLVADDDQSIRQLVSTIVKRENLLVDAVADGAE
                     AIEALKRHDYAVVLLDLMMPRLDGFGVIAWMKQNPPAVKPIVLVITAYADQSFKEVDP
                     NLVSGILRKPFEVADLGNLIRLCVSGFNEELSQKLFLSKDRAIRDFARGENDRRT"
     gene            complement(4277..6130)
                     /gene="asnB"
                     /locus_tag="VNN25_21340"
     CDS             complement(4277..6130)
                     /gene="asnB"
                     /locus_tag="VNN25_21340"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH94134.1"
                     /translation="MCGIYGMVADGAALRYPEVLGYMGEALRHRGPDGHAEFTRPDAV
                     IGTERLRIIDLHERADQPFAAPGAQVWLECNGEIYNAPEIRKRYPDYPYRSHSDVETI
                     LPLYLERGVAAIPELDGMFGLAIWDARTHTLILARDRAGEKPLFYAKVGGKSGAEVIF
                     ASELQCLLRHPEVSRELDPAAIGEYLRLGYIPEPRTPFRDVRRVEAGTFVRFTNSGQE
                     AVRYWDPESFRIQQIPAREAVAETRRLVERAVEKQVMSDVPVGVFTSGGLDSSILATL
                     AAKFIGVDKVHTYSAAFAEQSYDESPVAEELAKRIQTRYVPVPTDARTLLEALQSVVK
                     CVAEPLADPAILPTFLLARAARQHVKVVLSGEGADELFGGYPTYIGHKLAPVYDAMPS
                     FVRAILRRAVQRVPSSTKKVTLEFLLKRFVSDAEKPWIERHLAWFGTGLPADVFKLRA
                     ELPDLPVAPSSADPLAGAMLLDYRSYLRDNLLVKVDRATMLSSVEARAPFLDRDVTRF
                     ALSLPTDLRLRRFETKWVLKKASERWLPKDVIYRRKRGLSVPIASWLNDGLRSEVDRL
                     LAPERLRSQGLLDERIVGELLAAHRSGRANNAKPLWALVILQYWIENWSSR"
     gene            complement(6270..7880)
                     /locus_tag="VNN25_21345"
     CDS             complement(6270..7880)
                     /locus_tag="VNN25_21345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH94135.1"
                     /translation="MAGPSSLLRWWLLAVVVALSLHLGGFPLFDPDEGRNAEVGREMA
                     ATNDYVMPRLDGLPYLDKPIVYFAAEAAAMEVLGPTETAARLPALLFTLINAAFLWWF
                     VRRCVAPSGEAGDHQTDLIAAIVFLATPLAISFSRTVIFDSALALFITMATAFFYLAC
                     EEESRKWSGLAWLCIGLGVITKGPVALALPLLVAIPYAIWRKRFRALWSWSGLGLFIL
                     AVAPWVWAISLAIPDFLHYVLVTETAQRLATKALKRTGPPWYFIPYIIGGALPWSIVL
                     LGSPKAIDRRDRFTVFLVLWIAIPFLFFSISQSKRPQYILPLMAPVALLVARIWRDAR
                     KGSRVAAIVLLIAGAALVISPFVVHIPAEYGDAAKVSAIAIGATWIIGGALALMPRRD
                     VALVALTIPMIAIPLTANPIMQAIGARRSEAGLVEQLRPLVTPKTEVVGIDSFSGSMT
                     FYLQRTMIVVTPDAEELTSNYLIRHYATFAGGVSIKPESWLGNALNDAATPRIYIVRN
                     NDAPHRAQLAARGLRVVGGDARYTGYAR"
     gene            7914..>9020
                     /locus_tag="VNN25_21350"
     CDS             7914..>9020
                     /locus_tag="VNN25_21350"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXH94136.1"
                     /translation="MSGRVIAILFAAVAMVGVGSRVSGVGKTTAPHSDTRSPIIDSRA
                     ISAHIRFLASDALEGRETGMRGFEVAAEYVRAQFEAIGLQPLHDDWVQRFALRAAALD
                     EKASSLSINGAPLVIRKDYLLRPDFGRTDVTVDGSLVLAGFGVTAPELHQDDYAGIDA
                     RAKIVVVLSGAPKPFPSDQRAYYSSSDVKRQNAAAHGAVGMLTISTTTDEARSPFEKR
                     AKQSGIMPMTYLDSNGNPVDVVEAIAGSASLSRPTAESLFSSANLPLDRVLADAETGV
                     AHSFALAGTASIKTVTRFSEVRSENIIGVLRGSDPALRDEYVVVSAHLDHLGHHPQAD
                     GSDGLYSGAYDNASGIASLIEIARALAAGPRPKRS"
BASE COUNT         1765 a         2869 c         2708 g         1678 t
ORIGIN      
        1 gctggtatga gaaacgttcg acgtaacccc cttgtcatcg ccgtagcggt gctcttcttc
       61 ttcgccactt gcgcgaccgc gaagactcct cctccaccca aacttcccga atggaaagcc
      121 gtccccgcct cggttctcga ctcgttctgc gccaacttcc gcgaagaagg gatctcgtcg
      181 tcgaccacca tcaacgtcgt gaagacgtcc cagaagtacc tgatcacgcc cttgtcgatg
      241 caggctttgt ccgacttcgg tttctaccat gggtcgatgg actcagcgaa agcaacgagc
      301 gccgcactgg cctcgtcgga cgagatcccg gtaacgatgc ccaatgactg cgcctggcgt
      361 cccatcgaac caaagagcag cgcccgctac cccgacacga tgacgctcga gatctcgccc
      421 cccatcacca acccattctc ccgcaacgcc accggcctct tcgcccgcct aaccctcggc
      481 ggcgaagcct caacctggta ctggctcccc ctgatccccc gcggcgaaat ctggatcgcc
      541 gggaagatgg cgctgttgcc gtatcggcag tgaagggggc agcaacgcgc gttacgatgc
      601 ttcagtcagc aagtagcgtc tcgaatccgg cctgctggaa gtcccggtcg gcgctgagcg
      661 cttgcttgat tcccctctgt tccataatca agaacgacgt gcagtcggta aagccccacg
      721 tcttgtcaga gcggcggcgg taacgtccca gtgcttcccg gaaggcactg ctcgtcatcg
      781 gaaggatttc ggcgtccggg ctcgattgga tttgatcgac gagcaagctt gctcttgtgc
      841 ggagaaactc ccctttgctc gcgaatgcgt tgagaacctc ggccagtacc atctcgcttg
      901 tgacgatccg cgttcgcccg agcgtagcgg atgcctgccg agcctttgcg tgcagattgt
      961 ccttcggatt gacgatggca atccagtagt tggcgtccgc gaacacgctt ctcattcgtc
     1021 ctcgcgggac ctcccgtaca ggtagtgatc gagattggtc gcaagatcct ttggaacccg
     1081 gtcccactct tcaggaggga cacttgcgct gacgtcaatg attgactgcc agagcggttt
     1141 cgctgcgtcg tcgtaggtat cgttcatggt ttcctcctcg taagccggaa tctccgccac
     1201 cgccgagtcc ctgatctcga agtagcgggg agtagtctcc tccggagtca tggcctcccg
     1261 cctcggctcg taggacccgc cggacaacag cgccaaccgc cggcacgttt cacgtgtgag
     1321 ctccgccgcc gtagcggacc gcatcgcgcg cgtctcaatc gactccccct ggcgaaggct
     1381 ttttccgaag gctgccagca gcggtagtga acgcttcggc attcgatcgg gaataggccg
     1441 ccactcagac accgccgtaa tcgtctcgtc gattaacagc gccgcgtcgt cgatttcggc
     1501 aaagtcaccc aaagacttcc ccgagtgcgg gttgtcgcgt gagacgactc gcttgagcgc
     1561 gatcgcctcc gattccgatt cgatgaagta gaactttagt cggatcgatt tctgaaaacc
     1621 cctcggcaac cgcagtccgc cattcgcgtc tcgccacaac tctttggctg tttctacgag
     1681 gagcttccga tacgcgagca actccaagag cgcatcagcc tccaagcctg catcccaaaa
     1741 acgcggcccg gaaaacgtga tggtcgagac gtcgcgaatt tcgtcgctca tagttaccca
     1801 gcgagtccga ggatacacgg aaccggtacg ttgatccctc cacgtttagc cactccctgt
     1861 acagccggac gtgtctctca tcgaacatct accgctcgta gactcggagg cgaattccct
     1921 gtgagtgact gcgattcctg gcacggtccg gcatcacgga attcacggcg aggcgggacg
     1981 cctcgccgcc gcaggcgaga cgcctgcact ccttgggcgc cagtggggaa tccttatccc
     2041 cccggaagca cagagttctc agagtaagtc tccccctctg tgtcctcagt gtctctgtgg
     2101 ttcattcttt ttcacccgat tgctttagaa cccctgcgcc gtggctcacg ctgtccgcct
     2161 tcttcctacg cggacgaccc gtgcggccga gggctgactg catcgctatt acgtgggcga
     2221 cgagtgctgc ggaacggttg ttgcgggtgt atcccttggc gaggctgaac atctgcaacg
     2281 cctgcttcac gacttcccag cgctcccgca aaatgttgta gcgaatgtcc cgtgccagcg
     2341 cctcggcggc ggcggcgaga ggctcgaacc gcagattgcg gttgatgacc atccgcgttc
     2401 tggccggatc gagacccgtg gccgcttgca tcgactcgtc gttttcggca gcgacggcac
     2461 tggcttcgag aaactcgtcc gggacggtgc aggccgtccg cacctctctg aagccttcga
     2521 cgggcgccgg cacaaagtcg gacaggatct gatagatcgc ctgaacgtgc gctaccgcct
     2581 cctgcacgac ggcatcgagc gcctcaggat cgggcgttcc ggcgcctacg ctgtgaacgt
     2641 tttccgtggt ttgcatgacc tttctccttt gtcctcgatc gcctgacgga cccatccctg
     2701 agacgttttc ggattcaaaa cgtttctctt gatcggcaga cgacatctaa gtcacgcgat
     2761 tgcaattggt tacgcgttga tcctgaatcc aactgaatac gtacacaaat ggaatcaacg
     2821 cgttatcaca acgcatgaat gcgatatcgg aacgcatcaa cgcgaaacca gaacgcatga
     2881 atacgtagcc agaacgcatg aatgcgtagc tggaacgcgt gaatgcgtaa ccgcaacgcg
     2941 tgaatgcgaa accggaacgc atcaacggga aatcggaacg cgtgcacggt cacctcggta
     3001 tcatccggac aaacgaattg ctccgaggag ggctcatgct tccagtcatc gttcgctccg
     3061 ctgcttcgct tcgcaacgac attgaggctg ggccgcatcg gatgattgcg gatgagccct
     3121 tggccgccgg aggcacggag ttgggaccga cgccttacga ctttctcgct gccgccctcg
     3181 gcacgtgcac atcgatgacg atgcgcgtgg tcgcgaagaa ggaagacatc ccgctcgaag
     3241 gcgtcgagat cacgatcgaa aacgaccgga tgtacgcgaa ggattgcgcc gattgtctca
     3301 gcaccagcgg cttcatccac aggttcaccg tggccatcaa actcctcgga aacctgactc
     3361 cagcgcagcg agggcgtatg ctggacgtcg cggggcgctg tcctgtcaac aaaacactcc
     3421 gctcagagat ccgcatcgac gagcgtctcg tcgattgact tatccggatt tcttcctctc
     3481 acccacggag aacggattca tcggatgaca ccccccgcgg tggaaaccag gcaacgtgta
     3541 cttgtcgctg acgatgatca gtcgattcgt cagctcgtca gcacgatcgt caagcgcgag
     3601 aacctcctcg tcgatgccgt tgccgacggt gccgaggcga tcgaagcgct caagaggcac
     3661 gactacgccg tcgtcctgct cgatctcatg atgccgcggc tggacggctt cggcgtcatt
     3721 gcctggatga aacagaatcc gcctgcggtc aagccgatcg ttctcgtgat cacggcctat
     3781 gcggaccaga gtttcaaaga ggtcgatccc aacctcgttt ccggcatcct ccgcaagccc
     3841 ttcgaagtcg ccgacctcgg caatctgatc cgcctctgcg tgagcggctt caacgaggag
     3901 ctgagccaga aactgttcct gtcgaaagac cgcgccatcc gcgatttcgc gcgcggggag
     3961 aacgatcgga ggacgtagtt cgcggttgct tggttgctga tcggtgctgt ggttggtgtc
     4021 atcctgagac agccggagcc gccattcgtg ctcgatcaat ttgcgcggcg tgatctggcg
     4081 gcggaggacg gcgaaggatc tccaaatgcg aggcatgtgg catgtgcgtg gtaactggag
     4141 atccttcgcc gtcctccgcc tctgatcctg ccgattgcaa acgccgcgtg tgaggacggc
     4201 tccggctgtc tcaggatgac gcgaacgcgc tcgccgtcga gatcccacag tctcgcagga
     4261 atgtgacccc aattcgctac cgcgaagacc agttctcgat ccagtactgc aaaatgacca
     4321 gcgcccagag cggctttgcg ttgtttgcac gtcccgagcg atgcgccgca agcagttcac
     4381 cgactatgcg ctcatccagc aggccttgcg accgcaagcg ctctggcgcg agcaggcgat
     4441 cgacttcgga tcgcaatccg tcgttgagcc agctcgcgat cgggacgctc aaaccgcgct
     4501 tgcgccggta gatcacatcc ttcggcaacc agcgctcgga cgccttcttc agtacccact
     4561 tcgtctcgaa acggcgcagg cgcagatcgg taggaagcga cagggcgaag cgcgtaacgt
     4621 cgcggtcgag gaacggcgcg cgcgcttcga ccgacgacag catcgtggcc cggtcgactt
     4681 tcaccaacag gttgtcgcgt aggtagctgc gatagtcgag caacatcgct cccgccagcg
     4741 gatcggcgct cgaaggtgcg accggaaggt ccggcaactc cgcgcgaagc ttgaaaacat
     4801 ccgcgggcag tccggtgccg aaccacgcca gatggcgctc gatccacggc ttctccgcgt
     4861 cagaaacgaa acgcttgagg agaaactcga gcgtcacctt cttcgtcgat gatggcacac
     4921 gctgcacggc acggcgcaga atcgcgcgga cgaacgacgg catcgcgtcg tacaccggcg
     4981 ccagcttgtg gccgatgtac gtcggatatc cgccgaacag ctcatcggca ccttcaccgc
     5041 tgagaacgac tttcacatgt tgccgcgccg cccgcgcaag gaggaacgtc ggcagaatgg
     5101 cgggatcggc gagaggctcg gccacgcact tcacgacgct ctgcaaggct tcgagcaatg
     5161 ttcgcgcatc cgtcggcacc ggcacataac gcgtctgaat ccgtttcgcc aactcctcgg
     5221 ccaccggact ctcgtcatac gactgctccg caaacgcggc cgaatacgtg tgcaccttgt
     5281 cgacgccgat gaacttcgcg gccagcgtgg ccaggatcga ggagtcgaga ccgccgctcg
     5341 tgaaaacgcc gaccgggaca tcggacatga cctgcttttc aaccgcacgc tcgacgagac
     5401 gccgcgtctc cgcgaccgct tcgcgcgccg gaatctgctg gatgcggaac gactcggggt
     5461 cccagtagcg aaccgcttcc tggccgctgt tcgtgaatcg aacaaacgtt cccgcttcga
     5521 cgcgacggac atcgcgaaac ggcgtgcgtg gctcggggat gtagccgagt cggaggtact
     5581 cgccgatagc ggcaggatcg agctcgcggc tcacttcggg atgccgcaac aggcattgca
     5641 gctccgacgc aaagatcacc tcggcgcccg acttccctcc gaccttcgca taaaacaacg
     5701 gcttctcgcc cgcacgatcg cgcgccagga tgagcgtgtg cgtccgtgcg tcccagatcg
     5761 ccagaccaaa catcccgtcg agctccggga tcgccgccac gccgcgctcc aggtagagcg
     5821 ggagaatcgt ctcgacatcc gagtgcgagc ggtagggata gtcgggatag cgctttcgaa
     5881 tctcgggtgc gttgtagatc tcgccgttgc attcgagcca gacctgcgct ccgggcgccg
     5941 cgaagggctg atccgcccgt tcgtgaagat cgatgatgcg aagccgctcg gtgccgatga
     6001 cggcatccgg acgcgtaaac tccgcatgcc cgtccggccc gcgatgccgc agcgcttccc
     6061 ccatgtaccc caacacctcg ggataccgca gcgccgcacc atccgcgacc attccataga
     6121 ttccgcacat cgggggcgac tatagcaagg caagggggtt catccccgac gtgggtccat
     6181 ttgaaagccg ttaaccacag aggcacagag gtcacagaga cacctctgtg ctctctgtgc
     6241 ctctgtggtt gcctttttgt cctgagaacc taccgcgcgt aacccgtgta ccgcgcgtcg
     6301 ccgccaacga cgcgcaatcc tcgggcggcg agctgtgcgc gatgcggggc gtcgttgttg
     6361 cgcacgatgt agattcgcgg cgttgcggca tcgttcagcg cgtttccgag ccatgactcc
     6421 ggcttgatcg atacgccgcc ggcgaaggtg gcgtagtggc ggatgaggta gttgctcgtc
     6481 agctcttcgg cgtccggcgt gacgacgatc atcgtccgtt gcaggtagaa cgtcatcgag
     6541 ccgctgaacg aatcgatgcc tacgacttcg gttttcggcg tgaccagcgg acgcagttgt
     6601 tcaacgagtc ccgcctcgga acggcgagcg ccgattgctt gcatgatcgg attggcagtc
     6661 agcggaatcg cgatcatcgg aatcgtcagc gcaacgagcg cgacgtcgcg gcgaggcatg
     6721 agggcgagcg cgccgccgat gatccaggtc gcaccgatcg caatcgccga gaccttcgcc
     6781 gcatcgccat actccgcggg aatgtgtacg acaaatgggg agatgaccag cgctgcgccg
     6841 gcgattaaca gaacgatcgc cgccacacgc gagcccttcc gcgcgtcgcg ccagatgcgc
     6901 gcaaccagca gcgcgacggg cgccatgagc ggcaggatgt actgcggccg cttcgattgc
     6961 gagatcgaga agaagagaaa cgggatggcg atccagagca cgagaaagac cgtgaagcgg
     7021 tcgcggcgat cgatcgcttt cggcgagccg aggagaacga tcgaccacgg caaggcgccg
     7081 ccgatgatgt aggggatgaa gtaccacggc gggccggtcc gcttgagcgc cttcgtagcc
     7141 agccgttgcg cagtctcggt cacgagcacg tagtgcagaa aatcaggaat ggcgagcgag
     7201 atcgcccaga cccacggagc cacggcgagg atgaagagtc cgagcccgct ccatgaccag
     7261 agcgcgcgaa aacgtttgcg ccagatcgcg tacggaatgg ccacgagcag cggtagcgca
     7321 agcgcaaccg gccctttcgt gatgacgccg agtccgatgc agagccaggc caggccggac
     7381 catttgcgcg actcttcctc gcaggccagg tagaagaatg ccgtcgccat cgtgatgaag
     7441 agcgccaacg cgctgtcgaa gatgaccgtg cgcgagaagg agatggccag cggtgtcgca
     7501 aggaaaacaa tggccgcgat caggtcggtt tggtgatctc cggcctcgcc cgacggcgca
     7561 acacatcgcc taacgaacca ccacaagaac gccgcgttga tcagcgtgaa gagcagtgcc
     7621 ggcaggcgtg ctgccgtttc cgttgggccc agcacttcca tcgccgcggc ctcagcggcg
     7681 aaatagacga tcggcttgtc gaggtacggc aggccatcga gacgcggcat gacgtagtcg
     7741 ttcgtcgccg ccatctcccg gccaacctcg gcgttgcggc cttcgtcggg atcgaacagc
     7801 ggaaacccgc cgaggtgaag ggaaagggcg acgacgacag cgagaagcca ccagcgaagt
     7861 aaggaggaag gtccggccaa agcgggcgaa ggataccgct aatattcgcg gttatgtctg
     7921 gacgagtgat tgcgattctc ttcgcggccg tggccatggt gggtgtcggg agtcgggtgt
     7981 cgggtgtcgg aaagacgacg gcgcctcact ctgacacccg atccccgatc atcgattccc
     8041 gcgcgatttc tgcccacatc cgcttccttg cctccgatgc gctcgaagga cgcgagaccg
     8101 gcatgcgagg attcgaggtc gcggccgaat atgtccgtgc gcagttcgag gcgatcggac
     8161 tgcaaccgct gcacgacgat tgggttcagc gcttcgctct tcgggcggcg gcgctcgacg
     8221 agaaggcatc gtcgctgagc atcaacggtg cgccgctcgt cattcgcaaa gactatctgc
     8281 tgcgccccga ttttgggcgc accgacgtca ccgtcgacgg ctcgctggtg ctggccggat
     8341 tcggcgtcac ggcgccggag cttcatcaag acgactacgc cggcatcgac gcgcgcgcca
     8401 agatcgtcgt cgtactgagc ggcgctccga agccgttccc atcggatcag cgcgcctatt
     8461 actcatcctc ggatgtgaag cggcagaacg ctgctgcgca cggcgcggtg gggatgctga
     8521 cgatctcgac gacgaccgac gaagcgcgca gtccgttcga aaagcgggca aagcagagcg
     8581 gcatcatgcc aatgacgtat ctcgattcca acggaaaccc ggtcgatgtc gtcgaagcga
     8641 tcgcaggatc ggcgtccttg agccggccga ctgcggagtc gctcttctcg agtgcaaacc
     8701 tgccgctcga tcgcgttctc gctgacgcgg agaccggcgt cgcgcactcg tttgcgctgg
     8761 ctgggacggc atcgatcaag accgtgacgc gtttctccga ggtgcggagc gagaacatca
     8821 tcggagtgct gcgtggtagc gatcccgcgc ttcgcgacga gtacgtcgtc gtctccgcgc
     8881 atctcgatca cctcggccac catccgcagg ctgacggaag cgacggtctt tatagcggcg
     8941 cctacgacaa tgcctcggga atcgcatcgc tgatcgagat cgcacgcgct ctcgctgcgg
     9001 ggccgcgccc gaaacgatcg
//
LOCUS       DATLVW010000735         8392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_157181, whole genome shotgun sequence.
ACCESSION   DATLVW010000735 DATLVW010000000
VERSION     DATLVW010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157181"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VNN25_21355"
     CDS             complement(<1..269)
                     /locus_tag="VNN25_21355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXH94137.1"
                     /translation="MIEHEIEIKNKLGLHARAAAKLVHVAARFKCDIKIRKGDEEVDG
                     KSILGILLLAAGRGTMITIKANGEDEGDAVHAIEALINAKFDEVE"
     gene            complement(266..688)
                     /locus_tag="VNN25_21360"
     CDS             complement(266..688)
                     /locus_tag="VNN25_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94138.1"
                     /translation="MIAALIVTHGNLANELLSAAQKIEANLGVIEAVPLEWTDSVDEA
                     REKIRLALERVGTDSVIIFTDMFGGTPSNISLSFLEKDRIEIITGVNLPMIVKFATIH
                     QDGHDLTALAHAISERGAKSIRVASELLAADRRNGAPS"
     gene            complement(756..1430)
                     /gene="cmk"
                     /locus_tag="VNN25_21365"
     CDS             complement(756..1430)
                     /gene="cmk"
                     /locus_tag="VNN25_21365"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXH94139.1"
                     /translation="MPAPLIIAIDGPSGVGKTTTSRMVARELGIPHIDTGAMYRALAL
                     AAIREGIDTHDDAALEKLAQRVRIDFIAGETPHVLLNDEDVTSLIRTPEMSMAASNVS
                     AVPAVRRVLVKLQQDLGRRSGGVLEGRDIGTKVFPDTPFKFFLTARPEVRAQRRHDEL
                     AAKGEEVDYDSILAATMARDEQDSNRADSPLRYDDSYTVVDTSDLDIPAVVNAIVNGV
                     RARKTG"
     gene            complement(1440..2453)
                     /locus_tag="VNN25_21370"
     CDS             complement(1440..2453)
                     /locus_tag="VNN25_21370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXH94140.1"
                     /translation="MQQERLQKIIAHAGVSSRREAEAMIREGRVTLNGRVVTELGTRA
                     DPDRDHIKVDGKLITKAEPHRYILLYKPKEVMTTVEDPQGRRTVIDLVRGIRERIYPV
                     GRLDFHSEGLVLLTNDGELAFKVSHPTHGSVKTYSVKVRGVPEDRMIDKLRRGITLDG
                     KRTLPCDIERMRTTGKRDDEGNSWFEVKLREGRTQQIRKMFQAIGHPVSKLRRVAIGP
                     ITDPKLTPGVWRELTKHEVKMLETMKELKKPVKPQRATARPVSRKKPASAGAKRSAAR
                     PKARPAASRATATRSSTRRTPSPRPATPRPKIAAGKKPAGSKKASARRAPARPPRSSA
                     RRG"
     gene            complement(2453..3238)
                     /gene="scpB"
                     /locus_tag="VNN25_21375"
     CDS             complement(2453..3238)
                     /gene="scpB"
                     /locus_tag="VNN25_21375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXH94141.1"
                     /translation="MEPTELRAALEAILFVSNEPVKLDELAEAFAGEGREAVIEQLEE
                     IKRALDATLGGFMLEQTAGGWRFATRAEHDPILKKYFAKKGENRLSIAALETLAIVAY
                     RQPITAPEVSEIRGVNSNQTIRTLLERRMIRVAGRKNVVGSPFLYRTTREFLVHFGLN
                     DIRDMPRLEEFGDLIGEQINDDLVLAIETTHIAEGVEVPLEQGEQIDDAVNAELSAGD
                     ENLPPQETHAETPAEIHDEAHVDDDAQRATNEVQSEVVNDEHD"
     gene            complement(3295..4134)
                     /locus_tag="VNN25_21380"
     CDS             complement(3295..4134)
                     /locus_tag="VNN25_21380"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXH94142.1"
                     /translation="MTTMAEEEKHSAAVSQPADDRPLDEQEESGGALKVTLPSFHGPL
                     DLLLHLIKHHKIDIYDIPIALITEQYTGYLDAMEELSLDVAADYIYMAALLIHIKSKM
                     LLPHDETEGEVEDPRQELVDRLLEYQRFKAVAESFAELDVLRMGVWPRPVTTPPGMDA
                     TEIDMSEVGLFDLIDAFRSALNRYKQNHPQAIELRRTVHKISDKMRELYTKLQEKSPI
                     RLQWFLEGRDRNELIAIFLGTLELVRLGGISLKQSENFGEILIAGTDKTLEESVFEMF
                     DNS"
     gene            complement(4131..5138)
                     /gene="trpS"
                     /locus_tag="VNN25_21385"
     CDS             complement(4131..5138)
                     /gene="trpS"
                     /locus_tag="VNN25_21385"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXH94143.1"
                     /translation="MSQQPQYVLSGLRPTGRVHLGNYFGAVRNWVRLQDLFDCYYFVA
                     DWHALTSDYADTSKITQSTFDAVADWLAAGLDPAKATIFIQSEVKEHAELHLLLSMVT
                     PLPWLERVPTFKDQQAQIEDKDLNTYGFLGYPLLQTADIIIYRANFVPVGEDQASHLE
                     ISREIVRRFNNFYGDVFPEPQVLLTETPKVPGLDGRKMSKSYNNTIGLTASADEIRAL
                     VMTMFTDPERIRRKDPGRPDVCNLFQFHKLFSNEETIARVDHECRTAQIGCVDDKKLL
                     AEIIIESLRPLREKREAIDRDPSIVWDVLREGNRKARERAQQTMELVRDAIKLNYSEL
                     R"
     gene            5329..7017
                     /locus_tag="VNN25_21390"
     CDS             5329..7017
                     /locus_tag="VNN25_21390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH94144.1"
                     /translation="METPVLFLVDDDDAARARTGAALTRRFGSDYEVRTAANGDDGLA
                     ILKQLKDESRDVALVAASLDLRGVEGVEFLRRAHALHRKAARLLLLPTDEFHTHLPFT
                     TLPVLRRAMALGLIDLWIVKDWVTPEERFYPEVQEALTTWTVANRPHFAVYRIVGDQW
                     SPQSHSIRDVLGRNSIPFDFYAADSKQGQDLIRELSIDARRLPAAVRHDGTVLHNPTH
                     AELAESHGVQLKPSGGLYDLAIVGAGPAGLAAAVNAASEGLRTVIVERQAIGGQAGTS
                     SLIRNYLGFAHGISGRELAHQGWEQAIFFGADFVFNSAQELGRTGDGYALTLADGSKV
                     EARSVIVTSGVTYRRLDAPGLDRLLGAGVFYGAATVEAPAMAGESAFVVGGANSAGQA
                     ALHLAKFAANVVLLVRGGTLRSSMSEYLITHLQATPNIQIRYQTQVVEAHGDSQLESL
                     TIENIANGDRETIRAAGLFVMIGANPHTEWLTPALRVDDNGFILTGGDLPADAWPLDR
                     RPLSFETTLPGVFAAGDVRHLSVKRVAAAVGEGSVAVGSVHQYLALSDHEGDSF"
     gene            complement(7232..7648)
                     /locus_tag="VNN25_21395"
     CDS             complement(7232..7648)
                     /locus_tag="VNN25_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94145.1"
                     /translation="MAYVIGFAIAALVALFAYFFKFDRDRSFYPTVLIVVASYYVLFA
                     TMAGSARSIIVEVIVMMMFVGAAVAGMRSSQWFVVAGLALHGVFDFFHGSIIANAGVP
                     RFWPAWCLGYDVVAAACMAILVVSRMRPVVSEAVVS"
     gene            complement(7768..>8392)
                     /locus_tag="VNN25_21400"
     CDS             complement(7768..>8392)
                     /locus_tag="VNN25_21400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH94146.1"
                     /translation="IAGDLHTLLARAGEPGPYVLVGHSFGGLYVLNFAARYPEQVAGM
                     VLLDSTSPDQFTRVPQYPRFWDGYRRVSALFPSLGRFGIGRTAYRTSFDALPSQSRDE
                     QLAFWPTPRMARSQLDEWTAAPAVMRQAHALKSLGDRPLIVITAQREALDGWLPLQAE
                     LAKLSTNGVHRVQDATHQSIVESRVDSARSVEAIHEVVLAVRKNRGG"
BASE COUNT         1534 a         2590 c         2574 g         1694 t
ORIGIN      
        1 cattctactt catcgaattt cgcgttgatg agcgcttcga tcgcgtggac cgcatcgccc
       61 tcgtcctcgc cgttcgcttt gatggtgatc atcgtgcccc ggccggccgc gagtagcagg
      121 atgccgagaa tggacttgcc gtcgacttcc tcgtcacctt tgcggatctt gatgtcgcac
      181 ttgaagcggg ccgcgacgtg gacgagcttc gcggcggcgc gggcgtgaag gccgagcttg
      241 ttcttgatct cgatctcgtg ctcgatcacg aaggcgctcc gttacgacgg tccgccgcga
      301 gcaattccga agcaacgcga atcgactttg cacctcgctc gctgatcgca tgcgcaagcg
      361 cggtcaggtc atggccgtcc tgatgaatcg tcgcgaattt gacgatcatc ggcaggttca
      421 ctccggtgat gatctcgatc cggtcttttt cgagaaacga caggctgatg ttgctcggtg
      481 taccgccgaa catgtcggtg aagatgatga cggaatccgt tcccacacgc tcgagcgcca
      541 gccggatctt ctcgcgggcc tcatcgaccg agtcggtcca ttcgagcggc acagcctcga
      601 tcacgcccag gttcgcctcg atcttctggg cagcgctgag cagttcgttc gcgagattcc
      661 cgtgagtcac gatgagggca gcaatcaaac aagtctcctc ttgcagcggg cgaatgatag
      721 gtgggtgcgc cacgcggagc aaatgaggcg agtggtcatc ctgtctttct cgcgcgaacc
      781 ccgttcacga tcgcgttcac cacagcgggg atatcgagat cagacgtatc gacgaccgtg
      841 tacgaatcgt cgtaccggag cggcgaatcg gcgcgattcg aatcctgctc atcgcgcgcc
      901 atcgtcgccg caaggatcga gtcgtagtcg acctcctccc ctttcgcggc cagttcatcg
      961 tgccgccgtt gcgcgcgcac ctctggccgg gccgtcagaa agaacttgaa cggcgtgtcg
     1021 ggaaacactt tcgtgccgat gtcgcgccct tcgagcacgc caccgctgcg gcggccgagg
     1081 tcctgctgca gtttgacgag aacgcgccgc acggccggga ccgcggacac attgctcgcc
     1141 gccatgctca tctccggagt acggatgagc gacgtcacat cctcgtcatt caggagaacg
     1201 tgcggagtct ctccggcgat gaagtcgatg cggacacgtt gcgccaactt ctcgagcgcg
     1261 gcgtcgtcgt gcgtgtcgat cccctcgcga atcgcggcca gtgcgagcgc gcgatacatc
     1321 gcgccggtgt cgatatgcgg aatgccgagc tcccgggcca ccatgcggct cgtggtcgtc
     1381 ttgccgacgc cggaaggtcc gtcgatggcg atgatcaggg gcgctggcat gtgtgtggat
     1441 cagccgcggc gcgcactgct tcgcggcgga cgagcgggcg cgcggcgagc ggacgctttc
     1501 ttgctgccag ccggcttctt ccctgcggcg atcttcggac gtggtgtggc cggacgcggt
     1561 gatggtgttc ttcgcgtcga actgcgtgtt gccgttgcgc gtgaggccgc gggccgcgcc
     1621 ttcggacgcg ctgccgagcg cttggcgccg gccgacgcgg gcttcttgcg cgagaccggc
     1681 cgcgcagtcg cgcgttgcgg cttgaccggc ttcttcagct ctttcatcgt ctccagcatc
     1741 ttgacttcgt gcttcgtcaa ctcgcgccag actcccggcg tgagcttcgg atcggtaatc
     1801 gggccgatgg cgacgcggcg cagtttggag acggggtggc cgatggcctg aaacatcttg
     1861 cgaatctgct gcgtgcgccc ttcgcgcagt ttgacttcga accaactgtt gccttcgtcg
     1921 tcgcgcttgc cagtagtgcg catgcgctcg atgtcgcacg gcaacgtgcg ctttccgtcg
     1981 agggtgatgc cgcggcgaag tttgtcgatc atccggtctt cgggcacgcc gcgcactttc
     2041 acgctgtagg tcttcaccga tccgtgcgtc ggatgggaga ccttgaacgc gagctctccg
     2101 tcattcgtca gcaacaccaa tccctcggaa tgaaaatcga ggcggccaac gggatagatg
     2161 cgctcgcgaa tcccgcgcac cagatcgatg accgtgcggc gcccctgcgg atcctccacc
     2221 gtcgtcatca cttccttcgg cttgtaaagg aggatgtagc gatggggctc ggctttggtg
     2281 atcagcttgc cgtcgacctt gatgtgatcg cggtcaggat cggcgcgcgt tccgagctcg
     2341 gtgacgacac gcccgttcag cgtgacgcgt ccctcccgga tcatcgcttc cgcttcacgt
     2401 cgagacgaga cgccggcgtg ggcgatgatc ttctgcaagc gctcttgttg catcagtcgt
     2461 gttcgtcgtt gacgacttcg gattgtacct cgttcgttgc gcgctgcgcg tcgtcatcga
     2521 catgcgcctc atcgtgaatc tcagcgggag tctcagcgtg agtttcttgc ggcggcagat
     2581 tctcatcgcc tgcactcaac tccgcgttga ctgcatcatc gatctgctcg ccctgttcga
     2641 gaggcacttc gacgccttcc gcgatgtggg tcgtctcgat ggcgagaacg agatcgtcgt
     2701 tgatctgttc tccgatgagg tctccgaatt cttcgaggcg cggcatgtcg cgaatgtcgt
     2761 tgagtccgaa gtgaacgagg aactcgcgcg tggtgcgata gagaaacgga ctgccgacga
     2821 cgttcttccg tccggccacg cggatcatgc gccgctcgag caatgtgcga atggtctggt
     2881 tcgagttgac gccgcgaatc tccgacactt ccggcgcagt gatgggctgg cgatacgcga
     2941 cgatggccag cgtctcgagc gcggcgatgg agagacggtt ctcgcccttc ttcgcaaaat
     3001 acttcttgag gatcggatcg tgctcggcac gcgtcgcaaa gcgccagccg cccgcggtct
     3061 gctcgagcat gaacccgccg agggttgcat cgagggcgcg tttgatctct tcgagctgct
     3121 cgatcaccgc ttccctcccc tcgcctgcaa acgcctcggc aagctcgtcg agcttcaccg
     3181 gctcattgct gacgaagagg atggcctcga gggccgcacg taattctgtt ggttccattg
     3241 ccatcgaagg tcattacccc tgcgtgtcat cctgcgcctg acacgtctgt tgtcttacga
     3301 attgtcgaac atctcgaaca ccgactcttc gagcgtcttg tcggttccgg cgatcaggat
     3361 ctcgccgaaa ttctcgctct gcttcagcga gatgccgccg agccgcacca gctccagcgt
     3421 gccgaggaag atcgcgatca gctcgttgcg gtcgcgtcct tccagaaacc actgcaggcg
     3481 gatcggcgat ttttcctgaa gcttcgtgta cagctcgcgc atcttgtcgc tgatcttgtg
     3541 aaccgtgcgg cgcagctcga tcgcctgcgg atgattctgt ttgtagcggt tgagcgccga
     3601 tcggaaggcg tcgatcagat cgaagaggcc gacctcgctc atgtcgatct cggttgcatc
     3661 catgccaggc ggggtcgtta ccggccgcgg ccagactccc atccgaagga cgtcgagctc
     3721 ggcgaacgac tcggcgacgg ctttgaagcg ctgatattcg agcagcctgt cgaccagctc
     3781 ctgccgcgga tcttcgacct cgccttcggt ctcgtcgtgc ggcaacagca tcttcgactt
     3841 gatgtggatg agcagcgccg ccatgtagat gtagtcggcg gcgacatcga ggctcagctc
     3901 ttccatcgca tcgaggtagc cggtgtactg ctcggtgatc aatgcgatgg ggatgtcgta
     3961 gatatcgatc ttgtgatgct tgatgagatg cagcagcaga tcgagcggac cgtgaaacga
     4021 cggcagcgtg accttgagag cgccgccact ctcctcctgc tcatccagag gacggtcgtc
     4081 ggccggctgt gaaacggccg ccgagtgttt ctcttcttcc gccatcgtgg tcatcgcaac
     4141 tcggagtagt tcagtttgat tgcatcgcgc accaactcca tcgtctgctg ggcgcgttcg
     4201 cgcgctttcc ggttgccttc gcgcagcaca tcccacacga tcgatggatc gcggtcgatc
     4261 gcttcgcgtt tctcgcggag cggacgcagc gactcgatga tgatctccgc gagcagcttc
     4321 ttgtcgtcga cgcaaccgat ctgcgccgtg cggcactcat gatcgacgcg cgcgatcgtc
     4381 tcttcgttgg agaagagctt gtggaactgg aagaggttgc agacgtcggg ccggcccgga
     4441 tccttgcggc ggatccgctc cgggtcggtg aacatcgtca tcaccagcgc gcggatctcg
     4501 tccgcactcg cggtcagccc gatcgtgttg ttgtacgact tcgacatctt ccggccgtcg
     4561 agccccggga ccttcggcgt ttccgtgaga agcacctgcg gctccgggaa aacatcgccg
     4621 tagaagttgt tgaagcggcg cacgatctcg cgcgagatct cgagatgcga ggcctggtcc
     4681 tcacctaccg gaacaaagtt cgcccgataa atgatgatgt cggcagtctg cagcagcgga
     4741 tacccgagga agccgtaggt gttgagatcc ttgtcttcga tctgtgcctg ctgatccttg
     4801 aacgtcggca cgcgctccag ccacggcagc ggcgtcacca tcgacagaag caagtgcaac
     4861 tccgcgtgct ccttcacctc ggactggatg aagatcgtcg ccttggccgg atcaaggccg
     4921 gcggccagcc agtcggccac ggcatcaaac gtcgactgcg tgatcttcga cgtatcggcg
     4981 tagtcagacg tcagcgcgtg ccagtcggcg acgaagtagt agcagtcaaa aagatcctgg
     5041 agacggaccc agttcctgac cgcgccgaag tagttgccga ggtgaacgcg gcccgtagga
     5101 cggaggccgc taaggacgta ctggggttgt tggctcaatg ctgactcgtc gctcgatcgg
     5161 ggtctggaaa tttggaggcg gagtgtagca gaagcggaag gggtcaggtc gaaaccgaaa
     5221 ctgaggacgc gtcgcgcgct gacgaggaaa aagtgtggcg cctggtcgag gaaacgattt
     5281 cctccgctgc cgatctcggc ttggtcatgt aggctggcgc ccacagccat ggagactccg
     5341 gtcctctttc tcgtggatga cgatgacgcg gcccgcgctc ggaccggcgc ggcactgact
     5401 cgccgcttcg gctccgacta cgaagtccgc acggcggcaa atggcgatga tgggctggcg
     5461 atcctcaaac agctgaagga tgagagtcgc gacgtcgctc tggtcgcggc gagcctggat
     5521 ctccgcggtg tcgagggcgt cgagtttctt cgacgcgccc atgctctgca ccgcaaagcc
     5581 gctcgtcttc ttcttttacc cacagatgag ttccacaccc accttccatt cacgacgttg
     5641 ccggtcttgc gtcgagcgat ggcgctaggc ctcatcgacc tgtggatcgt gaaggattgg
     5701 gtgacgcccg aggaacgatt ctacccggag gtgcaggagg cgctgacaac atggacggtt
     5761 gccaacaggc ctcacttcgc tgtctatcgc atcgttggcg atcaatggtc gccgcaaagc
     5821 cacagcatac gagacgtgct gggccgcaac agcattcctt tcgatttcta cgcagcagac
     5881 tccaagcagg gacaggatct cattcgggag ctcagcatcg acgcccgtcg tcttcctgcc
     5941 gcggtgcgtc acgacggtac ggtcctccac aatccgacgc atgccgagct ggccgagtct
     6001 cacggcgttc agctcaaacc ttcgggcggc ctttacgacc tcgcgattgt cggtgcaggt
     6061 ccggcaggat tagcggctgc ggtcaatgcg gcatcggagg gactgcggac tgtgatcgtc
     6121 gaacgacaag cgatcggcgg ccaagccggt accagctccc tgatccgcaa ttacctcgga
     6181 tttgcgcacg gcatcagcgg cagggagctc gcgcatcaag gctgggagca agcgatattt
     6241 ttcggcgccg acttcgtctt caacagcgct caggagttag gccgcactgg tgatggatac
     6301 gcattgacgt tggctgacgg gtcgaaggtg gaggctcgtt ccgtaatcgt tacctcgggc
     6361 gtcacgtacc ggcgcctcga cgcccccggt ctcgaccgct tgctcggagc cggcgttttt
     6421 tatggcgccg ctacggtgga agcgccggcg atggccggcg aaagcgcgtt cgttgtcggc
     6481 ggagcgaatt cggccggtca ggcggcgttg catcttgcga agttcgccgc aaatgtcgtg
     6541 ctgctcgttc gcggcggcac tctccgcagc agcatgtcgg agtacctcat cacgcacctg
     6601 caagccacgc ctaacattca gatccggtac caaacgcaag tggtcgaggc gcatggcgat
     6661 tcacagctcg agagtctgac gatcgagaat attgcgaacg gcgaccgcga gaccatacgc
     6721 gccgcgggac tcttcgtcat gatcggagcc aatccgcata ccgaatggct gacacctgcg
     6781 ctccgtgtcg acgataacgg tttcatcctg accggaggag accttcctgc cgatgcttgg
     6841 ccgctcgatc ggcggcctct atcgtttgag acgaccctgc cgggagtctt tgccgccggc
     6901 gatgtccgcc atctatccgt caaacgcgtg gctgccgccg tcggagaagg ctcggtcgca
     6961 gtcggctccg tgcatcaata ccttgcgctc tcagaccacg aaggcgatag tttttgaatc
     7021 caaatgctgc cgacaacttg cacggcgatc attgtgagat gggcagccat gaacacggct
     7081 gccgggagag cgaccaggaa cggcggaaag cacagcacca cagcactgat tgtctccgca
     7141 aagctgatgt gacgcttcac cagccgatgg tgaaacaggt gcaacgcctg cacgacgagc
     7201 agagccacgt aggtgtgcgt tggagtcagc atcacgacac gaccgcttcc gagacaaccg
     7261 gccgcatgcg actcacgaca aggattgcca tacacgctgc tgcgaccaca tcgtagccaa
     7321 gacaccacgc cggccagaac cgaggtacgc cggcgttggc aatgatcgat ccgtgaaaga
     7381 aatcgaaaac gccatgcagc gccagcccag ccacgacaaa ccactgactg gatcgcatac
     7441 ccgcgaccgc ggctcccacg aacatcatca tcacgatcac ctcgacaatg atggatcgtg
     7501 ccgaccctgc catcgtcgcg aacagcacgt aatacgacgc gaccacgata agaaccgtgg
     7561 ggtagaagga tcgatctcga tcgaacttga agaaatacgc gaacaacgca acgagagcag
     7621 cgattgcgaa tccaatgacg tatgccacgt ggcctccggg tcaggtcaaa agtctaaaat
     7681 accgagtcag cctactcgac gcagcgacgc atcgctttcg gcactttaga ctttggacct
     7741 gaccgcaatc tacccgatgc agtgctccta tcctcctcga ttcttccgca ctgctaggac
     7801 gacctcgtgg attgcctcca cagaccgcgc ggaatcgacc cgactctcga cgatcgattg
     7861 gtgcgtcgca tcctgcacgc gatgcactcc attcgtcgat agcttcgcaa gctcggcctg
     7921 taacggaagc catccgtcga gggcttcgcg ctgcgcggtg atgacgatca gcggacggtc
     7981 gccgagactc ttcagcgcgt gcgcctgacg catcaccgcc ggcgccgcgg tccactcgtc
     8041 gagctggctg cgagccatgc gtggcgtcgg ccagaacgcg agctgctcgt cgcgggactg
     8101 cgagggcaga gcgtcgaacg aagtgcggta ggcggtcctt ccgatgccga agcgtcccag
     8161 cgacggaaaa agcgcactga cgcgccggta tccatcccag aacctcggat actgcggcac
     8221 tcgtgtgaac tggtcgggac tggtcgaatc gagcaacacc atcccggcaa cttgctccgg
     8281 atatctcgcg gcgaaattga ggacgtagag accgccgaag gaatgaccga caaggacgta
     8341 aggccccggc tcgccggcac gtgccagcaa tgtgtgcaaa tcgccggcga tc
//
LOCUS       DATLVW010000736        13875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_401088, whole genome shotgun sequence.
ACCESSION   DATLVW010000736 DATLVW010000000
VERSION     DATLVW010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401088"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VNN25_21405"
     CDS             complement(<1..369)
                     /locus_tag="VNN25_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH94147.1"
                     /translation="MHNLEHLFLAGEFPERARLLSGLTLEQVTLCPDGASHSIYEELW
                     HVVAYQQSIVAQGDPAGDRYPRAAPEHEQQWHDLVRVFLDGARTAAAMGQAPERLARE
                     IEPGLTMADELNSVAVHNAYH"
     gene            complement(546..794)
                     /locus_tag="VNN25_21410"
     CDS             complement(546..794)
                     /locus_tag="VNN25_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH94148.1"
                     /translation="MRKIEYKASVDRDLRRLDRQVATRIIDKIEQELTSADLRPNPLA
                     GPFEGLYKFRVGDYRVIYSLSADIVLVVRVAHRREVYR"
     gene            complement(781..999)
                     /locus_tag="VNN25_21415"
     CDS             complement(781..999)
                     /locus_tag="VNN25_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94149.1"
                     /translation="MTSYSIDLADDTASTLSEIAEVTAETPDALIRRAVEELVADYID
                     GRIALQRLDDPSDAVISSAELRRRFAED"
     gene            complement(1011..1994)
                     /locus_tag="VNN25_21420"
     CDS             complement(1011..1994)
                     /locus_tag="VNN25_21420"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXH94150.1"
                     /translation="MAYYSEHAQQLKRDLAAEVPTDELRVLHTKRPLLHGVIAFLNVA
                     ALVGAGVAIVHFDRWYLWLPFAAIAGFAVFDFTVLLHEVVHRAVLAEASERGYRFLGL
                     LYAVPSGISASQFTKWHLDHHAALGSSIDDPKRHYLSPKKNARWLKALYFTPALFPIY
                     FRAAAKETASYEPELRRRIARERLFTIAFQLSVLAAIALIGGWSIAFKLYIVPVFFIF
                     PIAFALNRLGQHYDIDPTDPAKWSTLVKGSWFWDVIYLFSNYHLEHHYFPGVPFYNLP
                     RLQKLLLPFYEKRGMTAHSYGALLWGWLILNRKPHANWDVAGAGVGAAVAE"
     gene            2047..2580
                     /locus_tag="VNN25_21425"
     CDS             2047..2580
                     /locus_tag="VNN25_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXH94151.1"
                     /translation="MQILTIGHSTRSQDEFLTLLGEHAIELLADIRRYPGSKRYPHFG
                     SGAMAVWLPENGIRYVHMPELGGRRKPLPDSPNTAWRNEQFRAYADYMATDEFRTAID
                     ALLELSAGARTAVMCAEAVPWRCHRNLVSDELTRRGIEVIHIIGSGSSRPHVMNSHAV
                     IKGEHLVYPAEQQTLRL"
     gene            2593..4611
                     /locus_tag="VNN25_21430"
     CDS             2593..4611
                     /locus_tag="VNN25_21430"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXH94152.1"
                     /translation="MAIPIVADPTIPEPKRDLVLAVDDNEQNLQLLEEYLSTWGYDVV
                     MARDGREALQLFPEINPSVIVLDVMMPVMDGYEACGSIKDQPGGRTIPILMLTALTGT
                     EDKIKALECGADDFLNKPINKDELRTRIRSLIRIRALREELDSSENIILTLTNALANK
                     DPRSGGHVERVAAYAARLSDKLGMSRSERETIVKGALLHDIGMIGVPDALLAKRDLTD
                     EERALVATHTRVGASILDPMTSFRQFVPMVRWHHERKDGRGYPDGLAGDAIPLEAQIV
                     ALANRYDEIRHGDGSSQDAALATLHDEAVDGAFDLELTSLFTTTLDEESPVPATTRHM
                     RPKQAPAAHVLCVDDNQVNRELVAASLTDAGFRVSFAENGAQAVEIIDNDGIDVVLLD
                     LLMPVQDGAETLRLLRADPRHEFLPVIVVTAQRGGTMRRDAIMAGADDFIVYPLNRLE
                     LITRIRSLLRIKDYHADLEQTHSVICALALALEAKDQYTRGHSQRVGDLARAFALYLD
                     LCDDVADRVRIAGLLHDIGKIAVPESLLNKQGPLTRDEFLRVIGHPVIGEEMVRPLNT
                     LSPVLGMIRHHHERYDGRGYPDGLRGENIPYEVRLLSIVDAYDALTSHRSYRPAPLSH
                     SAALETLSREAAGGKWDPKMVGQFAAMLGPNEPDWASITQPFPSLPVL"
     gene            complement(4757..5152)
                     /locus_tag="VNN25_21435"
     CDS             complement(4757..5152)
                     /locus_tag="VNN25_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94153.1"
                     /translation="MRRGESKACAQDSDLFRSGSPSDRLGNSLPSRNRQGQVDTLGSF
                     GSVEDELDIVNQNLVVVDQNLEFFIQNLDVDNQNLAVDDQNLVVDNQNLVVDDQNLDD
                     ADDKIGGKDTPNQSVGPCNGSIWTAYRGR"
     gene            5151..6122
                     /locus_tag="VNN25_21440"
     CDS             5151..6122
                     /locus_tag="VNN25_21440"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXH94154.1"
                     /translation="MILINDPLRQTDLPRGCVATIGNFDGMHIGHQAIIRGVVARARE
                     TGRPSAVITFQPHPLSIVAPDRVPPQILTLPQKHELLRDMGVDALLVVPFTHEFSRWT
                     ADRFISEFLVAALQITDVRIGRDFYFGAGREGNLAKLTAAGAQYGFAVQGVDEVKIRG
                     IRVSSSLVRDAIRKGTMRTVAAALGRTYFLDGRVATGRRLGRKIGFPTVNVDPSNELH
                     PGGGVYVTTSRFESFSRSFESVTNVGVRPTLYENYATTIESHILDFDSNVYGDTVRVY
                     FHELLRREQQFQSAVELTNQIRMDIERSRRYFLNHHTRYDEAGYSFP"
     gene            6126..7022
                     /locus_tag="VNN25_21445"
     CDS             6126..7022
                     /locus_tag="VNN25_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94155.1"
                     /translation="MSDPVLCIVVYAVFLAAMVAMHFFKTLDSGFGPAWRTALAMGVA
                     VAIVLFFVRGFYPIVGGVALTLAALYVRHTGQETEAIDGMIVGSAMGASAAISLVMTD
                     APVARMMAAMMMAGAVAGYGITFAVFHVADKRKQIAFDAATAAAAIVVAWLPSIAARY
                     RIPDRYTLFAVAGALPVIVIAAVFQQWPDVRAELRHEASLGFMSDRDVRATAHPLLRF
                     GRGGWADRRAHREFVRLANRIALRKRQQRGRTDEEARLYQLEIIKLRMQIQEMSRIDH
                     DVLSANVRDEVSSDTMTKTHEG"
     gene            7012..9357
                     /gene="lptD"
                     /locus_tag="VNN25_21450"
     CDS             7012..9357
                     /gene="lptD"
                     /locus_tag="VNN25_21450"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HXH94156.1"
                     /translation="MRVDTTPQRQAASRSTTPQLTARLLVAALVLAAAPAFLFAQVKD
                     MGPAGQYYNQPKPKFRVMPGLKKKGGEVKFSAKVNEQFVREEYAILDGDVVIEYQDVV
                     IHADKVTLNFKTKDVVAEGHVIMDQGPTRISGDHIVFNLDSKTGTFFNASGSMQPDMY
                     FIGDKIERLAEDHYLLTNGMFTSCDPDHPSWSFHVSSADITLDNYAHLRNVTLRAHDI
                     PLIYAPRLIWPTKSERSQGLLIPRVLLSKTYGDRLEMGYFIPFGDAADATIYADLNDK
                     GYNGVGVSTRYRPSDDVKLGDLSAYVVHDVQAGRNQWKYKYQHSQTNLPGGFRGVVDI
                     EDFSNLEFFRRYDRDPRLHTLSQIYSSAYLTKNSSRYSFNILTDRRDILLSNVDPNNP
                     LAAPPRQRFEQLPTLQFRLYPNRIGETPFYYSLESSMSHLATSGFVNGPTANYYRADV
                     FPTLSMQLRTPTWLSIRPQVSVRETYYSDSLDDASVANPFGKLNAVSNPVDRFYAQGQ
                     VEVVGPSFSKIFLKPLGGFSKFKHVIEPRFRYLYTTAVNDQQRIIRFDTVDSPFLPIV
                     RNSVEYSLTQRLIGRETGPNGNSREVASFSLTQSVSLGNAFTSATAGGLNGSGVPQTG
                     KFTPLVAALHVNPYSTVTLDAAATFGNVSHQLDQTSLSANLVGTGTRADKYLSFTWFS
                     SYKQPPVGGVPTFDSSSSQIRLNSGSSLFSNHIRADAQLNFDAKKGTFLEQRYLLGGT
                     ASCYGVAMEFRRYLVFVPEEKAEWSLGLSISLKNVGTIGTH"
     gene            9354..9710
                     /locus_tag="VNN25_21455"
     CDS             9354..9710
                     /locus_tag="VNN25_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94157.1"
                     /translation="MRRIVACALLLATFACSPAEHDRTRDEATLRAQLSSMRRAIAAY
                     TKSHNRGPSTLHEAMAHIPIDPFTHSATTWRLTTEETVQVDDFTTSTAAKRIEILEIH
                     SGAAGRDGSGKRYSEY"
     gene            complement(10082..10300)
                     /locus_tag="VNN25_21460"
     CDS             complement(10082..10300)
                     /locus_tag="VNN25_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010302960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXH94158.1"
                     /translation="MLDWSTCSAVERTPEKVSGAWVFAGTRVPVEALFVNLKDGARVN
                     DFLAWFPGVTREQIEAVLLHAGRSLAVA"
     gene            10379..10612
                     /locus_tag="VNN25_21465"
     CDS             10379..10612
                     /locus_tag="VNN25_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94159.1"
                     /translation="MSSPMSKEMAQGWMKAWQEVEDRQRRELQKETFDEKFESLAFLM
                     ESGGLFDLSQLDQEDQAARERWALLQSRWNRRI"
     gene            complement(10616..11014)
                     /locus_tag="VNN25_21470"
     CDS             complement(10616..11014)
                     /locus_tag="VNN25_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH94160.1"
                     /translation="MSRYCLDTSTYSHFKRGEPQAVELIDRAEWLGISTIVLGELGAG
                     FLHGAHRRPNEEQLAEFLSNRVVEVVGVDAEVSRHYAEIFVDLRRAGTPIPTNDIWIA
                     ATAARHGAILLTYDEHFNAISRVGSVVLRA"
     gene            complement(11011..11256)
                     /locus_tag="VNN25_21475"
     CDS             complement(11011..11256)
                     /locus_tag="VNN25_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94161.1"
                     /translation="MKNLTIRNVPDDLADALEREKSRRRESLNQTVIELLSQGLGVGL
                     SRSNGLARLAGTWTAEDQRMFEESIAPFEEIDAELWR"
     gene            11393..12067
                     /locus_tag="VNN25_21480"
     CDS             11393..12067
                     /locus_tag="VNN25_21480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH94162.1"
                     /translation="MPNVYTFKDFEGSSHRILIDLIRRFAPRGGTLLDLGAAGGELGS
                     AVRDQFDQTIGFEYDADRIGQLRSRFDQIVIADLETVRTLPSNVNAIVLADIIEHLRN
                     STNLLRMVKQSLTSDGVLFLSVPNIANITVRLGLLFGVFEYRDRGILDFTHLRFFTMR
                     TIRREIEKAGFHIVAIRGSSVPIRLIIGWMPDVLLRMGEHVLTWITRVWRGLFAYQII
                     LVAKVA"
     gene            complement(12136..>13875)
                     /locus_tag="VNN25_21485"
     CDS             complement(12136..>13875)
                     /locus_tag="VNN25_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94163.1"
                     /translation="IPLSQLVQRSGYLVSRANAAAEHVFPLRHFFLFLNPDHLGNWAL
                     HDWRGDRTLGVLNNYIEATVYLGIVALPLIFIAFANRRARSRWFWFAMLAVMLAAMFG
                     FMPVAKIVAMLPGFKFSPLTRIQMMLPIATAYLAAAGAALIARGRFRNAIAVLLAVAC
                     AADLAVFAGRFYPYVDPAVASPPTTPTIAFLQSQPKPFRIAPFFDDLWPNSSELYHLE
                     DVRSHFSSEASYRRLLERIDPSSALTGSTVINFNSLKFDFNDPLVSMLGIRYLLEQRT
                     IDVMKWTIFKYTKPGVQEIAAIVLEPGQVMQRHVRVVEEPFFAIEIPVEVQGEPGRNP
                     VLNVSLIKGANIVYARAFTPGDMAALNKIYIPLRPYARLGETVVLRLAANDVRVRVLT
                     GATDVAADAPLFYGRVTTPLIFDRELPDGRIFRNVGEVPRFHAVTRVRRMSADEMLAT
                     KDIDYADEAIVTDRRPMTVDASDAVVSLRSYATNEQVIDVVAPAKTFLASSEKLTPEL
                     QVTVDGSVVKPVEINVLFAGVPIPAGNHRVVFSRRLGRGWWLLAGIATILLLGLSAFD
                     IVSAKRKRVSPSA"
BASE COUNT         2700 a         4425 c         4206 g         2544 t
ORIGIN      
        1 gtgatatgcg ttatgcacgg ccacactatt caactcgtcg gccatggtca ggcccggctc
       61 tatttcgcgc gccaaccgct cgggcgcctg ccccatcgcc gccgccgtgc gcgcgccgtc
      121 caggaacact cgcaccaggt cgtgccattg ctgctcgtgc tccggcgccg ccctcgggta
      181 cctgtcaccg gccgggtccc cctgcgcgac gatactctgc tggtacgcca caacgtgcca
      241 cagctcctcg tagatcgagt gcgacgcgcc gtccggacat agcgtgacct gctcgagcgt
      301 cagcccggac agcaagcggg ctcgctcggg gaactcgccg gccagaaaca gatgttcgag
      361 gttatgcatc gcatcgctcc ttttcgccct atgttcgtca ccgcgactcg gcacaacgac
      421 gcacccggaa tcggaagcgc cgcacatctt cgagtcttcg aatccatcca aactctgcgt
      481 ttcgaactgt tcaatcggcc gtctgctgct ttatcccgcc ggccgcggag agtgggatgg
      541 tctgcctagc gatacacctc ccgtcgatga gcgacgcgga cgaccagaac gatatccgcc
      601 gatagtgagt agatcacgcg atagtcgccc acacgaaact tgtaaagccc ttcgaaggga
      661 ccggccaggg gattgggacg aagatcggcg gaagtcagct cttgctcgat cttgtcgatg
      721 atgcgcgttg cgacttgacg gtcaagccgg cgcagatcgc gatcgacgga ggccttgtat
      781 tcaatcttcc gcaaaacggc gtcgaagctc cgcggaggaa ataaccgcat cggacgggtc
      841 gtcaagccgc tgcaatgcga tgcgaccgtc gatgtagtcg gcgacgagct cttccactgc
      901 tcggcgaatg agcgcgtcgg gcgtttccgc ggtcacttca gcgatctcgc tcagagtaga
      961 ggcggtgtcg tcggcaagat caatcgagta cgaagtcatg agagggaatt ctactccgcc
     1021 accgccgccc ccacacccgc ccctgccaca tcccaattcg cgtggggctt gcgattcagg
     1081 atcagccatc cccataagag cgcgccgtaa ctgtgtgcgg tcatgccgcg cttttcgtag
     1141 aacgggagga ggagcttctg taaacgtggc aggttgtaga acgggacgcc ggggaagtaa
     1201 tggtgttcga ggtggtagtt cgagaagagg tagatgacgt cccagaacca ggagcctttg
     1261 acgagggtcg accacttcgc gggatcggtg gggtcgatgt cgtagtgctg gccgaggcgg
     1321 ttgagggcga aggctatggg gaagatgaaa aagaccggca cgatgtagag cttgaacgcg
     1381 atgctccagc cgccgatgag ggcgatcgcg gcgaggacgc tgagctggaa ggcgatcgtg
     1441 aaaaggcgct cacgggcgat gcggcgtcgc agctcgggct cgtaggatgc ggtctccttc
     1501 gcggctgcgc ggaagtagat cgggaaaagc gccggcgtga agtagagggc tttgagccag
     1561 cgcgcgtttt tcttcggcga gaggtaatgg cgcttcggat cgtcgatcga ggagccgagg
     1621 gcggcgtgat gatcgagatg ccacttcgtg aactgcgagg cggagatgcc gctgggaacg
     1681 gcgtagagca gccccaggaa gcggtaaccg cgctcgctcg cctcggcgag cacggcgcga
     1741 tgcacgacct cgtgcagcaa cacggtaaag tcgaacacgg cgaagccggc gatggccgcg
     1801 aacggcagcc agaggtacca gcggtcgaag tggacgatgg ccaccccggc tccgacgagc
     1861 gcggcgacgt tcaggaacgc gatgactcca tgcagcagcg ggcgcttcgt atgcagcacc
     1921 cgcagctcat ccgttggcac ctcggcagca aggtcgcgtt tgagctgctg ggcatgctcg
     1981 ctgtagtaag ccatgaacgg gaaatagaac caccctgggc ggagcattat tcaacagatc
     2041 ggtctcatgc agatcctcac catcggccac tccacgcgct cgcaggatga attcctgacg
     2101 ctcctcggcg agcacgcgat cgagctgctg gccgacatcc gccgctatcc cggatcgaag
     2161 cgctatccgc acttcggaag cggcgcgatg gcggtctggc tgccggagaa tggcatccgc
     2221 tacgtccaca tgccggagct gggcggccgc cgcaagccgc ttcccgactc gccgaacacg
     2281 gcttggcgca atgagcagtt tcgtgcctac gccgactaca tggccacgga cgagttccgg
     2341 accgcgatcg acgcactgct ggagttgagc gccggcgcgc ggaccgcggt catgtgtgcc
     2401 gaggcggtgc cgtggcgctg tcatcgcaac ctggtttccg atgagctgac gcggcgcggc
     2461 attgaggtca ttcacatcat cgggagcgga tcgtcacggc ctcatgtcat gaacagtcat
     2521 gccgtcatca aaggggaaca tctcgtgtat cccgcggagc aacagaccct gcggctataa
     2581 tcgccgcctc gaatggccat cccaatagtg gctgatccaa cgattcccga accgaagcgc
     2641 gacctcgtcc tcgccgtcga cgacaacgaa cagaacctcc agttgctcga ggagtacctc
     2701 tccacctggg gttatgacgt cgtgatggca cgtgacggcc gcgaagcgct gcagctcttt
     2761 cccgagatca acccctcggt catcgtgctc gacgtgatga tgccggtgat ggacggctat
     2821 gaggcctgcg gctcgatcaa ggatcagccc ggcggacgga cgattccgat cctgatgctc
     2881 acggcgctga ccggcacgga agacaagatc aaggcgctgg agtgcggtgc cgacgatttc
     2941 ctgaacaagc cgatcaacaa agacgagctt cgcacccgca tccgctcgct gatccggatc
     3001 cgcgcgctgc gcgaggagct cgattcgagc gagaacatca ttctcacgct gaccaacgcg
     3061 ctggcgaaca aagacccgcg cagcggcggt cacgtcgaac gcgtggcggc ctatgcggct
     3121 cgcctgagcg acaaactcgg catgtcccgc agcgaacgcg agacgatcgt gaaaggcgcg
     3181 ctgctgcacg acatcggcat gatcggcgtc cccgacgcgc tgcttgcgaa gcgggatttg
     3241 actgacgaag agcgagcgct cgtggccacg cacacccgcg tcggcgcatc gatcctcgac
     3301 ccgatgacga gtttccgcca gttcgtgccg atggtgcgct ggcatcatga gcggaaggat
     3361 gggcgcggct atcccgatgg ccttgccggc gacgcgatcc cgctagaggc ccagatcgtc
     3421 gccctcgcca accggtacga cgagatccgc cacggcgatg gttcgtcgca ggatgcggcg
     3481 ctggcaacgc tgcacgatga agcggtcgat ggcgcgttcg acctcgagct gacgtcgttg
     3541 tttacgacga cgctcgatga ggaatcgccc gtcccggcga ccacgcgtca catgcggccg
     3601 aagcaggcac cggcggcaca cgtgctctgc gtggacgaca atcaggtcaa ccgggagctt
     3661 gtcgcagcca gcctcaccga cgccggattc cgcgtctcgt tcgccgagaa cggcgcccag
     3721 gcggtcgaga tcatcgacaa cgacggcatc gacgtcgtgc tgctcgacct cctcatgccg
     3781 gtccaggacg gcgcggagac gctgcgtttg ttgcgcgccg atccgcgcca cgagttcctt
     3841 ccggtcatcg tcgtcaccgc gcaacgcggc ggcacgatgc ggcgcgacgc gatcatggcc
     3901 ggcgccgacg atttcatcgt ctacccgctc aaccggctgg agttgatcac gcgcatccgc
     3961 tcgctgctgc ggatcaagga ctatcacgcc gacctcgagc agacccacag cgtgatctgc
     4021 gcgctggcgc tggccctcga agcgaaagac cagtacacgc gaggccactc gcagcgcgtc
     4081 ggcgatctcg cccgcgcctt cgcgctgtac ctcgatctgt gcgacgacgt ggcagaccgc
     4141 gtccgcattg ccggcctgct tcacgacatc ggcaagatcg ccgttccgga atcgctcctg
     4201 aacaaacaag gtccgctgac gcgcgacgag ttcctgcgcg tcatcggcca tccggtgatc
     4261 ggcgaagaga tggtgcgtcc gctgaacacg ctctcgccgg tgctgggaat gatccgccac
     4321 caccacgagc gctacgacgg ccgcggctat cccgacggct tgcgcggcga gaacatcccg
     4381 tacgaggtcc gccttctctc gatcgtcgac gcctacgacg ccctgacgtc acaccgctcg
     4441 tatcgccccg cgccgctgtc gcattcggca gcgctggaaa cgctctcccg cgaagccgca
     4501 ggcggtaaat gggacccaaa gatggtcggc cagttcgccg cgatgctggg gccgaacgag
     4561 ccggactggg cgtcgatcac gcagccgttt ccgtcgctgc cggtgttgtg agggtgactg
     4621 atccgttgag atccttcgac cgtccgtcta caccgattga gagcagcgaa tcttgcggaa
     4681 aaaccggtca ccacagaggc acagaggtca cagagatccc ctctgtgttc tctgtgtctc
     4741 tgtggttgcc tttgcttcag cgaccacgat aagccgtcca aatcgaccca ttacacggcc
     4801 ccaccgattg gtttggcgta tcttttccac caattttgtc atcagcatca tccagattct
     4861 ggtcatcaac aaccagattc tggttatcaa caaccagatt ctggtcatca acagccagat
     4921 tctggttatc aacatccaga ttctggatga agaactccag attctggtca acgacgacca
     4981 gattctggtt gacgatatcg agttcgtctt caacgctacc gaacgaaccc aaggtgtcaa
     5041 cctgaccttg acgattcctt gacggcagac tgtttccgag acgatcgctg ggtgatccgc
     5101 tccgaaacag atcggagtcc tgcgcgcatg ctttagactc gccgcgccgc atgattctca
     5161 tcaacgaccc acttcgccag accgatctgc cgcgcggctg tgtggccacc atcggcaact
     5221 tcgatgggat gcacatcggg catcaggcca tcattcgggg cgtcgtcgcg cgggcgcgcg
     5281 agacagggcg gccgtccgcg gtgatcacgt ttcagccgca tcccctctcg atcgtcgcgc
     5341 ccgaccgcgt gccgccgcag attctgaccc tgccgcagaa acatgagctg ctacgcgaca
     5401 tgggcgtcga cgcgctgctc gtcgtcccct tcacgcacga gttctcgcgc tggacggccg
     5461 accgcttcat ctctgaattc ctcgtcgcgg cgttgcagat caccgacgtg cgcatcggac
     5521 gcgatttcta cttcggcgcc ggacgcgaag ggaatctcgc caagctgacg gcggccggcg
     5581 cgcaatatgg cttcgccgtt cagggcgtcg acgaggtgaa gatccgcggc attcgcgttt
     5641 ccagctcgct ggtgcgcgac gccatccgca aaggaacaat gcgcacggtg gcggcagcgc
     5701 tgggccgcac ctacttcctc gatggccgcg tcgctacggg ccggcggctc gggcgcaaga
     5761 tcggattccc gaccgtcaac gtcgatccat cgaacgagct gcatcccggc ggcggcgtct
     5821 acgtcacgac gtcgcgtttc gaaagcttca gccgctcgtt cgagagcgtg accaacgtcg
     5881 gcgtccggcc gacgctctac gaaaactacg ccaccacgat cgagagtcac atcctcgatt
     5941 tcgactccaa cgtctacggc gacaccgttc gcgtctactt ccacgaactg ctccgccgcg
     6001 agcagcagtt ccagtccgcg gtggagctga cgaatcagat ccgcatggac atcgaacgct
     6061 cgcgccgcta cttcctcaac catcacacgc gctatgacga ggctggatac tcgtttccgt
     6121 aaaccatgtc cgatcccgtt ctctgcatcg tcgtctatgc tgtttttctg gccgccatgg
     6181 tggccatgca cttcttcaaa acgctcgaca gcggcttcgg gcctgcgtgg cgcaccgcgc
     6241 tggcgatggg cgtcgcggtc gcgatcgtgc ttttcttcgt gcgcggcttc tacccgatcg
     6301 ttggcggtgt ggcgctgaca ctggcggcgc tgtatgtccg ccacaccggc caggaaacgg
     6361 aagcgatcga cgggatgatc gtcggcagcg ccatgggcgc ctcggcggcc atttcgctgg
     6421 tgatgacgga cgcgccggtg gcgcggatga tggccgcgat gatgatggcc ggcgcggtgg
     6481 ccggttacgg catcaccttc gccgtctttc acgttgccga caagcgaaaa cagatcgctt
     6541 tcgatgccgc aaccgcggct gctgccatcg tcgtggcgtg gctgccgtcg atcgccgccc
     6601 gctaccggat tcccgatcga tacacacttt tcgcggtcgc cggcgccctg ccggtcatcg
     6661 tgatcgccgc ggtcttccag caatggcccg acgttcgcgc cgagctgcgc cacgaagcct
     6721 ccctcggatt catgagcgac cgcgacgtac gcgccacggc gcatccgttg cttcgcttcg
     6781 gccgaggagg ttgggcggac cggcgggctc atcgtgaatt cgtgcggctc gccaatcgca
     6841 tcgcccttcg caaacggcag cagcgcggcc gcaccgatga ggaggctcgt ctttatcagc
     6901 tcgagatcat caaactgcgc atgcagatcc aggaaatgag ccgcatcgac cacgatgtgc
     6961 tttcagcaaa cgtcagagat gaagtgtcgt cggatacaat gacgaagacg catgagggtt
     7021 gataccaccc cgcaacgcca ggccgcctca cgcagcacaa cgccacaact gacggcgcgc
     7081 ctcctcgtcg ccgccctcgt gctcgccgcc gcgcccgcct tcctcttcgc gcaggtcaag
     7141 gacatggggc cggccggtca gtactacaac cagccgaagc cgaagttccg cgtcatgccc
     7201 ggtctgaaaa agaagggcgg cgaggtgaag ttcagcgcga aggtgaacga gcagttcgtc
     7261 cgcgaggagt acgccatcct cgacggcgac gtcgtcatcg agtaccagga cgtcgtcatt
     7321 catgccgaca aagtgaccct caatttcaag accaaagatg tcgtggccga ggggcacgtc
     7381 atcatggatc agggaccgac gcgaatcagc ggcgatcaca tcgtcttcaa tctcgactcg
     7441 aagaccggta ccttcttcaa cgccagcggc tcgatgcagc cggacatgta tttcatcggc
     7501 gacaagatcg agcggctggc cgaggatcat tacctgctga cgaacgggat gttcacctcg
     7561 tgcgatcccg accatccctc gtggtctttc cacgtgtcca gcgccgacat cacgctcgac
     7621 aactacgcac atctgcgcaa cgtcaccctc cgcgcgcatg acattccgct gatctacgcg
     7681 ccgcggctca tctggccgac gaagagcgag cgctcgcagg gactgctcat tccgcgcgtc
     7741 cttctctcga aaacgtacgg cgaccggctg gagatgggct acttcattcc gttcggagac
     7801 gccgccgacg ccacgatcta cgccgatctg aacgacaagg gttacaacgg cgtcggcgtg
     7861 agcacgcgct accggccatc ggacgatgtg aagctcggcg atctcagcgc ctatgtcgtg
     7921 cacgacgtgc aggccggacg caatcagtgg aagtacaagt accagcactc gcagaccaat
     7981 ctgccgggcg gcttccgcgg cgtcgtcgac atcgaagact tctcgaacct ggagttcttc
     8041 cgccgctacg accgcgatcc gcgtctgcac acactctcgc agatctactc ctcggcctat
     8101 ctgacgaaga atagcagccg ctattcgttc aacatcctca ccgaccgccg cgacatcctg
     8161 ctgagcaacg tcgatccgaa caacccgctt gcggccccgc cgcgtcaacg cttcgagcaa
     8221 ctccccaccc tgcagttccg gctctacccg aaccgcatcg gagaaacgcc gttctattac
     8281 tcgctcgaat cgtcgatgtc gcatctggcc acgagcggat tcgtcaacgg cccgaccgcg
     8341 aactattacc gcgccgacgt ctttccgacg ctttcgatgc agcttcggac gccgacgtgg
     8401 ttgtcgatcc gcccgcaggt ctcggtgcgc gagacgtact actccgactc cctcgacgat
     8461 gcctcggtcg ccaatccctt cggcaagctc aacgcggtca gcaatccggt cgaccgtttc
     8521 tacgcgcagg gacaggtcga agtcgtcggg ccgtcctttt cgaagatctt cctcaagccg
     8581 ctgggcggtt tcagtaagtt caagcacgtg atcgagccgc gcttccgcta tctctacacc
     8641 accgccgtga acgaccagca gcgcatcatc cgcttcgaca cggtcgactc gccgtttctg
     8701 ccgatcgtgc gcaactcggt ggagtactcg ctcacgcaac gactgatcgg acgcgagaca
     8761 ggcccgaacg gcaactcgcg cgaggtggcg tcgttctcgt tgacgcagtc cgtctcgctc
     8821 ggcaacgctt tcaccagcgc gacggcaggt ggactgaacg gttcgggcgt cccgcaaacc
     8881 ggaaaattca cgccgctcgt cgccgctctg cacgtgaatc cgtattcgac ggtgacgctc
     8941 gacgcagcgg cgaccttcgg gaacgtgtcg caccagctcg atcaaaccag cctctccgcg
     9001 aacctcgtcg ggaccggcac ccgcgccgac aagtacctga gcttcacctg gttctcgtcc
     9061 tacaagcaac cacccgtggg cggcgtgccc accttcgatt ccagctcgag tcagatccgc
     9121 ctcaacagcg gttcgtcgtt gttcagcaac cacatccgcg ccgacgccca gctcaacttc
     9181 gacgcaaaaa aaggcacctt cctcgagcaa cgctacctcc tcggcggcac cgcctcctgc
     9241 tacggcgtcg ccatggagtt ccgccgctac ctggtcttcg tccccgaaga aaaagcggag
     9301 tggagcttag gcctctcgat ctcgctgaag aacgtcggga cgatcggcac gcattgagac
     9361 gtattgtcgc gtgcgcgcta ttgctcgcca cgttcgcatg cagcccagcc gaacatgatc
     9421 gcacccgcga tgaagccacg cttcgtgcgc aactgtcatc gatgcgccgc gcaatcgccg
     9481 catacacgaa atcgcacaac cgcggccctt cgacgctgca cgaagcgatg gcccacatcc
     9541 cgatcgatcc attcacgcac tccgcaacaa catggcgcct gaccaccgag gagaccgtgc
     9601 aggttgacga cttcacgacg agcaccgccg ccaaacggat cgagatcctc gagattcaca
     9661 gcggcgctgc cgggcgggac gggagcggga aaaggtattc ggagtactga gtcaaggaag
     9721 catcagctca acgaagtcac caggccggag gtcgtcaacc gtttgggcga tcttcgccac
     9781 acccttgtta aattgatccc ctctcccatc gagcgccttc tcccccacgc tttttgtggg
     9841 ggagaaggtg gcgaagccgg atgagggggc gttccaacga gtacaacctg cgtgcatctg
     9901 gcgcgttttc aggcagttcg gactgaggcg tggttgatcg ctctgcgcat ttgctccccc
     9961 ctcatccggc ttcgccacct tctccccccg caaaaagcgc ggggggagaa ggcgctcgat
    10021 gggagagcac tccaaagaaa aggaagaggt gagcaaggag gcgagtcacg cgcgtcggtt
    10081 atcaggcaac ggccaggctg cgcccggcgt ggagcagcac ggcttcaatc tgttctcgag
    10141 ttacgccggg aaaccatgcg aggaagtcat tcacgcgggc gccgtctttc agattcacaa
    10201 agagagcctc gactggaact cgcgtgccgg cgaaaaccca ggcgccgctg actttttcgg
    10261 gcgtccgttc aaccgcagag cacgtgctcc aatcaagcat gtgaaaaggc taaccgatgc
    10321 aatgcacgcc agcaagttcg taagccacgt ctccaagccc gaccattaca atggccactt
    10381 gagcagtcca atgtcgaagg aaatggctca aggatggatg aaggcgtggc aggaagtgga
    10441 agatcgtcag cgccgggagc tccaaaaaga aaccttcgat gagaagtttg agtcgctcgc
    10501 gtttctgatg gaatcaggcg ggctttttga tctctcgcaa ctcgatcagg aagatcaggc
    10561 agcgcgagag cgttgggcgc ttcttcagtc tcgatggaac cggcggatct gaccgctacg
    10621 cacgtaggac tactgagccg accctcgata tggcgttgaa gtgctcgtcg taggtcaaga
    10681 ggatggcgcc gtgccgagcc gcggtcgctg cgatccagat atcgttggtt ggaatcggtg
    10741 tgccggctcg acgcagatcg acgaagattt ccgcatagtg tctgctgact tccgcatcga
    10801 ctccgactac ctcaacgacg cggttcgaga gaaactcggc gagttgctcc tcgttcggtc
    10861 ttcggtgcgc tccgtgaagg aaccccgctc ccaactcgcc taatacgatc gtcgagattc
    10921 ccagccactc ggctcggtca atgagctcca cggcttgtgg ctcgcctcgt ttgaaatggc
    10981 tgtaagtcga cgtgtcgaga cagtaacgac tcatcgccag agctcagcgt cgatctcttc
    11041 gaaaggcgca atgctctctt caaacatccg ctggtcctcc gcagtccagg taccagcgag
    11101 acgcgccaaa ccattcgaac gggacaagcc aacgccgagg ccttggctaa gcagctcgat
    11161 caccgtctgg ttaagagatt ctcgtcggcg gctcttctcc ctctcgaggg cgtcggcgag
    11221 atcatcgggc acgtttcgga tcgtgaggtt cttcaatcaa gctcccgtgc agtcaacaca
    11281 tgcatcctat gactgcattt ctatgcggtc aaccttctga cggcaactct cacacgaaga
    11341 gatccccgtc aatgttgtgt tccctcaccc catccactac cctcccgcga tcgtgccgaa
    11401 cgtctacaca ttcaaggatt tcgaagggag cagtcatcgg atcctcatcg atctgattcg
    11461 ccgcttcgcg cctcgcggcg gaacactcct cgacctcggc gcggcgggcg gcgagcttgg
    11521 gtcggccgtg cgcgaccagt tcgatcagac tatcggcttc gagtacgacg ccgatcggat
    11581 cggacagctt cgcagccgtt tcgatcagat cgtcatcgct gatctggaga cggttcggac
    11641 tctgccatcg aacgtcaacg ccatcgttct ggccgacatc atcgagcacc tgcgcaactc
    11701 gacgaacctg ctgcgcatgg tcaagcagtc actgacgagc gacggtgtcc tgttcctctc
    11761 cgtgccgaat atcgccaaca tcaccgtccg cctcggctta ctgttcggcg tgttcgaata
    11821 ccgggaccga ggaattctcg atttcaccca tctccggttt ttcacgatgc gcacgatccg
    11881 ccgcgagatc gaaaaggccg gctttcacat cgtggccatc cgcggttcgt cggtccccat
    11941 ccgactgatc atcggctgga tgccggatgt gttgctgcgt atgggcgagc acgtgctgac
    12001 ctggatcacg cgcgtctggc ggggactgtt cgcgtatcag atcatcctgg tggcaaaggt
    12061 ggcgtgacgc ctggctttac gcctgaacca ccgccgggca gggacgcccg gcggatgcag
    12121 gcaagaatgc ctgcactacg ccgacggtga cactcgtttg cgcttcgcgc tcacgatgtc
    12181 gaaggccgat aacccgagga gcagaatcgt cgcgattccg gcgaggagcc accatccgcg
    12241 gccgaggcgt cgcgagaaca cgacgcggtg attgccggcg ggaatgggga cgccggcgaa
    12301 gagcacgttg atctcgaccg gtttcacgac agagccgtcg acagtgacct gcaactccgg
    12361 cgtcagtttc tccgacgatg ccaggaacgt tttcgccggg gcgacgacgt cgatgacctg
    12421 ttcgttcgtc gcgtagctgc gcagcgaaac gacggcatcg gaggcatcga cagtcatcgg
    12481 ccgacggtcc gtgacgattg cctcatcggc gtagtcgatg tcctttgtgg ccagcatctc
    12541 gtccgcgctc atgcggcgca cgcgcgtgac ggcgtggaag cgcggaacct cgccgacgtt
    12601 gcggaagatg cggccgtcgg gcagctcgcg gtcgaagatc agcggcgtcg tgacgcggcc
    12661 gtagaacaac ggcgcgtcgg ccgctacatc tgttgcgccg gtcaggacgc gcacgcgcac
    12721 gtcgttggct gccaatcgca gaacgacggt ctcgccgagc ctcgcgtacg ggcgaagcgg
    12781 gatgtagatc ttgttgagcg cagccatgtc gcccggcgtg aacgcgcgcg cgtagacgat
    12841 gtttgcgcct ttgatcagcg acacattcaa aaccgggttg cggccgggct ctccttgaac
    12901 ctcgacagga atctcgatcg cgaagaacgg ttcttcgacg acgcgcacat gccgttgcat
    12961 cacttggccc ggctcgagga cgatcgcggc gatctcctgc acgcccggct tcgtgtactt
    13021 gaagatcgtc cacttcatga cgtcgatggt tcgctgttct agcaggtagc ggatgccgag
    13081 catggacaca agcggatcgt tgaagtcgaa tttgaggctg ttgaagttga tgacggtcga
    13141 tcccgttagc gcggagctcg gatcgatccg ttcgaggagg cggcggtagc tcgcttccga
    13201 ggagaagtgg ctgcgcacgt cttcgaggtg atagagctcg gaactgttgg gccagagatc
    13261 gtcgaagaag ggcgcgatgc gaaacggttt cggctgcgac tgcagaaacg cgatcgttgg
    13321 cgtggtcggc ggcgaagcta ccgccggatc gacgtaggga taaaagcggc ccgcgaatac
    13381 tgcaagatcg gcggcgcagg cgacggcgag cagcaccgcg atcgcgttac ggaaccggcc
    13441 gcgcgcgatg agcgcggcgc ccgcggcagc gaggtacgcc gtggcgatgg gcagcatcat
    13501 ctggatgcgc gtcagcggcg agaatttgaa tcccggaagc atggcgacga tcttcgccac
    13561 cggcatgaag ccgaacatcg ccgcgagcat cacggcgagc atggcaaacc agaaccaacg
    13621 cgatcgtgcg cggcgattgg cgaaggcgat gaaaatcagg ggaagcgcga cgatgccgag
    13681 gtagaccgtc gcttcgatgt agttgttgag gacgccgagg gtgcggtcgc cgcgccagtc
    13741 gtggagtgcc caattgccga ggtggtcggg attgaggaag agaaagaagt ggcggagagg
    13801 aaagacatgt tccgccgccg cgttggcgcg cgagacgaga taaccgctgc gctgcacgag
    13861 ctgcgagagg gggat
//
LOCUS       DATLVW010000737         5126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_401111, whole genome shotgun sequence.
ACCESSION   DATLVW010000737 DATLVW010000000
VERSION     DATLVW010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401111"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VNN25_21490"
     CDS             complement(<1..253)
                     /locus_tag="VNN25_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HXH94164.1"
                     /translation="MHFPLSFDPRTWHWDLLAAFGFIAQALFAARFIVQWIASERRKM
                     SHVPVGFWYLSLCGGMLMTIYGVARRDPVIILGQAPALIV"
     gene            complement(310..648)
                     /locus_tag="VNN25_21495"
     CDS             complement(310..648)
                     /locus_tag="VNN25_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH94165.1"
                     /translation="MASRLAERLERISELGGITGRDIAQLLDTTPETVSRWSSGKIDP
                     QRDRLQRLLDLEVFLSELAEFYSPEEVKLWLFSPHKLLGGKTAAARIQAGETQDVFAV
                     LDQLRSGAYA"
     gene            complement(661..1380)
                     /locus_tag="VNN25_21500"
     CDS             complement(661..1380)
                     /locus_tag="VNN25_21500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH94166.1"
                     /translation="MASPDLSVVFPVYNEEENIPILLDEIAKALDESPWSYEVVAVDD
                     GSTDRSLEVLQESKAKHPGLRVLTFTKNSGQTAALDAAWRAARGTYVVSLDADLQNDP
                     ADIPAMMRKLEESTADMVIGVRVNRHDTWSRKMQSKIGNGVRNWITNDHITDTGCSLK
                     LIKREAIDRVRLHTGMHRFLPTLVRMQGYKVVEMPVNHRARQFGVSKYGAMNRAFRGL
                     ADCMAVRWMSKRVLRYEVREE"
     gene            complement(1380..2138)
                     /locus_tag="VNN25_21505"
     CDS             complement(1380..2138)
                     /locus_tag="VNN25_21505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXH94167.1"
                     /translation="MIFTDSHCHLTMSDAEGNLDRARAGGVRGFVVPGTKLSDAPQAV
                     AVAQRHDDVWAAVGFHPHEAKDCDDVAFAEIERLAGESRVVAIGECGLDYHYMHSTRE
                     TQIAVFERHLDLAKRVNKPIIVHNRESTEDMVSILTKAGARGILHSFTESEEVARKFI
                     DAGYFISFSGIVTFRSAESLRACAKALPHDRVLIETDTPYLAPVPFRGRDNEPAYVVK
                     VAELLATLWGIPLDEVAAQTTANFETVFGVTLPR"
     gene            complement(2222..3412)
                     /locus_tag="VNN25_21510"
     CDS             complement(2222..3412)
                     /locus_tag="VNN25_21510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXH94168.1"
                     /translation="MPKLNETIYLLAAVRTPIGKFGGGLSSLTAVQLGIASARATLER
                     SGVDPAAVDEVIFGNARQAGVGPNPARQIAIKSGLAHETPAYTVNQACGSGLRTIMNA
                     ADQIRLGHASIVLAGGTESMSNTPYMLPRARWGYRMGDAEVVDGMYRDGFMCPLADEL
                     MGATAETLADRYEISREEQDAYAVETQRRAGVAYDEGRFHDEIVPVTVAGRKGDVVID
                     RDEHPRFDADLGSLAKLPPVFRKNGTVHAGNSSGVTDGASSMLVASAEAVKRHGLKPM
                     ARIIGYAQAGVDPKVMGIGPVPATKKLLAETGIALSEIDLIELNEAFAAQVLACDREL
                     HLDRDKVNVHGGAISLGHPIGATGARIITTLVHALSSRDKRLGLATLCISGGMGISML
                     VEKC"
     gene            complement(3400..4407)
                     /locus_tag="VNN25_21515"
     CDS             complement(3400..4407)
                     /locus_tag="VNN25_21515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXH94169.1"
                     /translation="MFDPISFAKRLIDIPSPTDDEFDVAVFLHDELTALGYACRRQEV
                     SERRFNLYAAAGGRPRVVLNSHVDTVPPWFGASEDGEALYGRGACDTKGIIAAMIAAG
                     ERLRASGMSDFGFLFVVGEETDSIGAKMANQEFGALGSEYVLVGEPTQSTFARASKGA
                     LTCFVRFDGIAGHSAYPERGDSAINKMVAAIAEINATQWGVDDDLGATTVNVGVVRGG
                     EKPNVIPAEAECQMIFRTVTDPAIVQAKLQAIITRHGGTITVSRGNPPQFMTVPDGAK
                     SVVVAFNTDVPHLGALGKPLLFGPGSILDAHGANEKIGKKELLDSIETYVETVHALCR
                     N"
BASE COUNT          959 a         1633 c         1542 g          992 t
ORIGIN      
        1 acacgatcag tgccggagcc tgaccgagaa tgatgacggg atcgcgccgt gccacgccat
       61 agatcgtcat gagcattccg ccgcaaagcg acaagtacca gaacccgacc ggcacatggc
      121 tcattttccg ccgctccgac gcgatccact gcacgatgaa ccgcgcggca aaaagcgcct
      181 gcgcgatgaa cccaaacgca gccagcaggt cccagtgcca cgtgcgcgga tcgaacgaaa
      241 gcgggaagtg cattgggtgc agtttacgcg gtcacgcggt cttgccgttc gcccgactga
      301 aaaacagtcc tacgcgtacg cacccgagcg cagttggtcg agtactgcga acacatcctg
      361 agtctctccg gcctggatac gcgctgccgc cgtcttgcct cccaacaact tgtgagggct
      421 gaagagccag agcttcactt cttcgggtga atagaattct gcaagctcgc tgagaaagac
      481 ctcgagatcg agaagacgtt gcagccggtc gcgttgcgga tcgatcttgc ctgagctcca
      541 tcgcgaaact gtttcaggag tcgtgtcgag cagttgcgcg atgtcgcggc cggtaattcc
      601 gccgagctca ctgattcgtt caagccgctc tgcaagccgt gaagccatga tgactggact
      661 ctactcctcc cgcacctcgt accgcagcac gcgcttcgac atccagcgca cggccatgca
      721 gtcggcgagg ccgcggaagg cgcggttcat cgcgccgtat ttcgagacac cgaactgccg
      781 ggcgcggtga ttgaccggca tctccactac cttgtagccc tgcatccgaa cgagcgtcgg
      841 caggaagcga tgcatgccgg tgtggagacg gacacggtcg atggcctctc gtttgatcag
      901 cttcagcgaa cagccggtgt ccgtaatgtg atcgttcgta atccagttgc gaacgccgtt
      961 gccgatcttc gactgcattt tccgcgacca cgtgtcgtgc cggttcacgc ggacgccgat
     1021 caccatgtcg gccgtgcttt cctcgagctt gcgcatcatg gccgggatgt ccgccggatc
     1081 gttctgcaga tcggcgtcga gcgacacgac atacgtgccg cgcgcggcgc gccatgccgc
     1141 atcgagcgcc gccgtctggc cgctgttctt cgtgaacgtc agaacgcgca gcccgggatg
     1201 cttcgccttg ctctcctgca aaacttccag actgcgatcg gtcgagccat catcgacggc
     1261 gaccacttcg tatgaccacg gcgattcatc cagcgccttc gcgatctcgt cgagaaggat
     1321 cgggatattt tcttcctcgt tgtagacagg aaagacgacg gagaggtcgg gcgacgccat
     1381 cagcggggca gtgtaacgcc gaagaccgtt tcgaaatttg ccgtcgtctg tgcggccacc
     1441 tcgtcgagcg gaatgcccca caacgtcgcc agcagctccg ccactttgac gacatacgcc
     1501 ggctcgttgt cgcgtccgcg gaatgggacc ggcgcgagat acggcgtgtc ggtctcgatc
     1561 aggacgcggt cgtgcggcaa tgccttcgcg caggcacgca gcgactcggc gctgcgaaac
     1621 gtgacgatgc cggagaacga gatgaagtag ccggcgtcga tgaacttccg cgccacctcc
     1681 tccgactcgg tgaacgagtg aaggatgccg cgggctcccg ctttggtcag aatcgacacc
     1741 atatcttccg tcgactcgcg gttgtgcacg atgatcggct tgttgacccg cttcgccaga
     1801 tcgaggtgtc gctcgaagac ggcgatctgg gtctcgcgcg tggagtgcat gtagtgatag
     1861 tcgagcccgc actccccgat cgcgacgacg cgagactcgc cggccagccg ctcgatctcc
     1921 gcgaatgcca cgtcatcgca atccttcgcc tcatgcggat gaaaaccgac agctgcccag
     1981 acatcgtcgt gacgctgcgc cactgcaacc gcttgcggcg catcggagag cttcgtgccc
     2041 gggacgacaa atccacgcac gcctcctgcc cgcgcacgat caaggtttcc ctcggcgtcg
     2101 gacatcgtga gatggcagtg agagtcagta aagatcatga ttttttctta tccgcagatg
     2161 acgcagatag acacagataa aaggcaaatc tcaatctgcg tttatctgcg tcatctgcgg
     2221 atcagcattt ctccacgagc atcgatatcc ccatcccccc gctgatgcac aacgtcgcca
     2281 ggccaagccg cttgtctcgt gatgaaagag cgtggacaag cgtcgtgatg atcctcgcgc
     2341 ccgttgctcc gatcggatga ccgagcgaga tcgctccgcc atgcacgttt accttgtcgc
     2401 ggtcgaggtg aagctcgcgg tcgcacgcca gcacctgcgc ggcgaaggct tcgttcagct
     2461 cgatgagatc gatctccgac aacgcgattc ccgtctccgc gagcagcttc ttcgtcgcag
     2521 gaaccgggcc gatgcccatc accttcggat cgacgcctgc ctgtgcgtag ccgatgatgc
     2581 gcgccatcgg cttcaacccg tggcgcttca ccgcctccgc cgacgcgacc agcatcgatg
     2641 acgcaccatc cgtgacgccc gacgagttgc cggcgtgcac cgtcccgttc ttgcggaaca
     2701 cgggagggag cttggcgagt gacccgaggt cggcgtcgaa gcgcggatgt tcatcgcggt
     2761 cgatgacgac gtcgcccttc ctgccggcaa ccgtcaccgg cacgatctca tcgtgaaacc
     2821 tgccctcgtc gtacgcgacg ccggcgcggc gctgcgtctc cacggcatac gcatcctgtt
     2881 cttcgcgcga aatctcgtag cgatcggcga gcgtctccgc ggtcgcgccc atcagctcgt
     2941 cggccaacgg acacatgaac ccgtcgcgat acatcccatc gacgacctcg gcatctccca
     3001 tccgatagcc ccagcgcgca cgcggcaaca tatacggcgt gttcgacatc gattcggtac
     3061 cgccggccag aacgatgctg gcgtggccga gacggatttg atcggcggcg ttcatgatgg
     3121 tgcgcaatcc cgagccgcag gcctggttga ccgtgtaggc cggcgtctcg tgcgccaggc
     3181 cgctcttgat cgcgatctgc cgggccgggt tcggccccac tcccgcctgg cgtgcattgc
     3241 cgaagatcac ttcatcaaca gcggccggat cgacaccgct gcgctcaagt gtggctctcg
     3301 cgctcgcaat ccctaactgc acggcggtga gcgatgacag accgccgccg aacttgccaa
     3361 tcggcgtgcg gacggcggcg agcagataaa tcgtttcgtt cagtttcggc atagcgcgtg
     3421 gaccgtttcg acgtaggtct ctatcgagtc caacaactcc ttcttcccga tcttctcatt
     3481 tgcgccgtgt gcatcgagga tcgatcccgg accgaacagc agcggcttcc caagcgcgcc
     3541 gaggtgcggc acgtcggtat tgaatgcgac gacgacggat tttgctccat cgggcactgt
     3601 catgaactgc ggcggattac cgcgcgacac ggtgatcgtg cccccgtgcc gcgtgatgat
     3661 ggcctgcagc ttcgcctgca cgatcgccgg atccgtgacg gtgcggaaga tcatctggca
     3721 ctccgcttcc gccggaatca cgttcggttt ctctccccct cgcacgacgc cgacgttcac
     3781 ggtggtggcg ccgagatcat catcgacgcc ccactgcgtg gcattgatct ccgcgatcgc
     3841 cgcgaccatc ttgttgatcg ccgagtcacc gcgctcgggg tatgcggagt gaccggcgat
     3901 gccatcgaag cgcacgaagc aggtcagggc tccttttgaa gcgcgcgcaa aggtcgattg
     3961 cgtcggctcg ccgacgagga cgtattcgga tccgagagcg ccgaactcct ggttggccat
     4021 cttcgcgccg atcgagtcgg tctcctcgcc gacaacaaaa aggaatccga agtcgctcat
     4081 cccgcttgcc cgcagccgct cccccgcagc gatcatcgcc gcgatgatgc ccttggtgtc
     4141 gcacgccccg cgcccgtaaa gcgcttcccc atcctcggat gctccgaacc acggcggaac
     4201 ggtatcgaca tgagaattga gcacgacgcg cggccttccg ccggcagcag cgtaaagatt
     4261 gaagcgacgc tcgctgacct cttgtctgcg gcatgcgtag ccgagggcgg tcaactcgtc
     4321 gtgcagaaag acggcgacat cgaattcatc gtccgtcggc gacgggatat cgatcaggcg
     4381 cttggcgaat gagatggggt cgaacatgag cgcggcggat tgtatcgggc tcgaacggcg
     4441 gggaggaatt cgtgggcggc gtaaaacaga aaggacacgg catagtcgat cagaactggt
     4501 gcgttcaatc aatcaagtac agcgcgtttc tcgaacgaat cgtgtcatcc tgagactgcc
     4561 ggagccgccc gcgcgaatcg cgatgtttgc gcaacacgtg ctcggcggcg taggacgtcg
     4621 aaggatctca agtttcgcag atcgtgttcg tctcgagcgg tggtgatcat tgacgttcgc
     4681 tcgcgctggg ccaacgagat cttcgtagtt tgagatcctt cgatcgtcct acgccgcgat
     4741 gcctgccggt gcaaatgcag cgtggaggcg cggctccggc gactcaggat gacaccacac
     4801 ggtttgcgcg tggttagggt tgagtgtcat ccggagttga tcgtccaatc gcgattggtg
     4861 tcatcctgag actgccggag ccgcccttcg tactcgatcc ttcgcgacgc gtggagtggc
     4921 ggcgaaggac ggcgaaggat ctcaagtttc gcagatcgtg ttcgtctcgc gcgagcgttt
     4981 gcgaccattg acgttcgctt gggctgggca aacacgatcc gtaaagtttg agatccttcg
     5041 atcgtcctac gccgcgatgc ctgccggtgc aaatgcagcg tggggcgcgg ctccggcgac
     5101 tcaggatgac accacacggt ttgcgc
//
LOCUS       DATLVW010000738         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_30289, whole genome shotgun sequence.
ACCESSION   DATLVW010000738 DATLVW010000000
VERSION     DATLVW010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30289"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..>2278
                     /locus_tag="VNN25_21520"
     CDS             286..>2278
                     /locus_tag="VNN25_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94170.1"
                     /translation="MKKISLALSVVFLFLASVAVAQGPPVTPPGLAHFPSLDEQLKKA
                     HAIPGSALDALIRANQDFGKLKAKDADDLIVPAWLKVYWRKGHPEANYDNDNDPTGGY
                     PLVLKEVLEWMMSHQDLKPGQPSASMAPGRNFGDLDEEEAPTHTTMGRFGPIAALNAT
                     AGTNVRTSGAQSNARSESDIRINYWNPSKIIAASNNISGSGTQGEYYSSDGGVTWGQT
                     NLPLASGDSFHSDPTVEWTSDGTAWSSTMGINGSVLKVQSYRSANGGATWTFDGTISG
                     TQTNTDKQMEWADHSGTSAFANNIYVIWHNGNPAFMNRRTSSGWGAPIQVSDSQATGT
                     CIGADVKTNSGGDVFGFFPDTGSRGIFVVKSTNGGTSYGTPVKLVTTYDSYDIGVPSF
                     SSRRILLYVSGGAYKNGTTNNVYALWTDLSGDTGCTTAANEPGTNAASTCKTRIWFSR
                     STDGGATWSARVKLNNQSGLNDQFNPFLAVDETNGTLGAMYYDTVNDPNRKKVDVYYQ
                     MSTDGGATWQAAVKVTTAMTDETTAGADSGNQFGDYNSLSGYANSFFPSWTDRRNNAS
                     EEIWTAKITTSTTPTFSISGNAGTTGATVTAGTGSATSDSSSNYSIANLAAGTYTVTP
                     SKSGCTFSPTSTSVTITSANVTGINFTATCTAPTFTISGN"
BASE COUNT          500 a          781 c          630 g          367 t
ORIGIN      
        1 tccataactc ctcggtccga tgagtcgagc cacctcggca gcaagccacg acattgtccc
       61 tcggagaaga aaaatgcgcc tcgcggtggg ttcgcccgat atggctccaa gggtgggtca
      121 tcctttgcct cactgctcaa cattgtgacc tcccctgacg cccttaaggg tcaggaaagc
      181 taccaaaaga gagcaaatcg ggctcttttg tgacggcacg gctcttgatc tagctcagcg
      241 gtaatcagtt acaaaatttg aacgttttat gaaggggaat gttacgtgaa gaaaatctct
      301 ctcgccctga gcgtcgtctt tctgttcctg gcatcggtgg ccgttgccca gggtcctcca
      361 gtcactcctc ccggtcttgc ccactttccc tcgctcgatg agcagctcaa aaaggctcac
      421 gccattcccg gcagcgcgct cgacgcactg attcgcgcga atcaggactt cgggaagctc
      481 aaagccaagg acgctgacga cttgatcgtt ccggcctggc tcaaggtcta ctggcgcaag
      541 ggacatcccg aagccaacta cgacaacgac aacgatccga cgggcggcta cccactcgtt
      601 ctgaaagaag tactcgagtg gatgatgagc caccaggatc tgaagccggg gcagccctcg
      661 gccagcatgg cgccgggacg taacttcggc gatctcgatg aggaggaagc gcccacgcac
      721 accacgatgg gacgcttcgg gccgatcgct gcgctcaatg ccaccgcggg aacgaacgtc
      781 cgcacctcgg gcgcgcagag caacgcccgc agcgagtccg acatccgcat caactactgg
      841 aacccgagca agatcatcgc cgcatcgaac aacatcagcg gctccggtac gcagggcgag
      901 tactactcga gcgacggcgg cgtgacgtgg ggtcagacca acctgccgct cgccagcggc
      961 gactccttcc actccgatcc gaccgtcgag tggacctccg acggcacggc ctggtcgagc
     1021 acgatgggca tcaacggaag cgttttgaaa gtccagtcgt accgctcggc caacggcggc
     1081 gcgacatgga cgttcgatgg aaccatctcc ggaacgcaaa ccaacaccga caaacagatg
     1141 gaatgggccg atcacagcgg cacgtcggcg ttcgcgaaca acatctacgt catctggcac
     1201 aacggcaacc cggccttcat gaaccgccgc acctcctcgg gatggggagc gcccatccaa
     1261 gtcagcgact cgcaggccac aggcacgtgc atcggcgcgg acgtgaagac caactccggc
     1321 ggcgacgtct tcggcttctt ccccgacacc ggctcgcgcg gcatcttcgt cgtcaagtcg
     1381 accaacggcg gcacgagcta cggcacgccg gtgaagctcg tcaccaccta cgactcctac
     1441 gacatcggcg tgccgtcatt ctcgagccgc cgaatccttc tctacgtctc cggcggcgcg
     1501 tacaagaacg gcacgaccaa caacgtctac gcactctgga ccgatctctc aggcgacacc
     1561 ggctgcacga cggctgcgaa cgagcccggc accaacgcgg cctcaacctg caagacgcgc
     1621 atctggttct cgcgctcgac ggatggcggc gccacgtgga gcgcccgcgt aaagctcaac
     1681 aaccagagcg gtctgaatga ccagttcaac ccgttccttg ccgtcgatga gaccaacggc
     1741 actctcggcg ccatgtacta cgacaccgtc aacgatccga accgcaagaa ggtcgacgtc
     1801 tactatcaga tgtccaccga tggcggcgcg acgtggcagg cggccgtgaa ggtcacgacc
     1861 gcgatgaccg acgaaacgac cgcgggtgcc gactccggca accagttcgg cgattacaac
     1921 agcctctccg gctacgccaa ctcgttcttc ccctcatgga ccgaccgccg caacaacgcc
     1981 agcgaagaga tctggaccgc gaagatcacg acgtcgacga ctccgacgtt ctcgatctcg
     2041 gggaacgccg gaacgaccgg cgccaccgtc acggccggaa ctggatcggc cacgagcgat
     2101 tcgtcgagca actactccat cgccaacctc gccgctggca cctacaccgt gacgccgagc
     2161 aagagcggct gcaccttctc gccgacttcg acctccgtca cgatcacctc ggccaacgtg
     2221 accggcatca acttcaccgc aacgtgtacg gcgccaacct tcacgatcag cggcaacg
//
LOCUS       DATLVW010000739        18561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_365464, whole genome shotgun sequence.
ACCESSION   DATLVW010000739 DATLVW010000000
VERSION     DATLVW010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18561
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365464"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VNN25_21525"
     CDS             complement(<1..997)
                     /locus_tag="VNN25_21525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH94171.1"
                     /translation="MNLGRVISSQKRAVLLVIGLLSIAGLVALARIPRALFPQTDFPR
                     VIIVADNGVAPAQQTLVSVTKPIEEAMSGIPGIARIKSTTARGSAEVDLFFDWKTDIV
                     QTLQLVQTRISQLASKLPPGATIERTERLTFAVFPIIGYSITSPVRDPGTLRNLAEVT
                     LRPPIARIDGVASVEVLGGQLREYHVLIDRSRLEARGVSLQQVSDAVRGSNVINSPGL
                     INENHQLELALVSGQATSLDDLAAIVVTTVNNVPVRVGDVATVSPGLEPRFTIVTADG
                     RPAVLLNVMRQPTANILSVAGGVKAELEGLRRQLPRDVEIKPYYDQSLLVSGSVSS"
     gene            complement(997..2346)
                     /locus_tag="VNN25_21530"
     CDS             complement(997..2346)
                     /locus_tag="VNN25_21530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH94172.1"
                     /translation="MSSTLMNAPGSDEEPLWRRRRVQIGAAIGLLILIVAGWCWHRHN
                     AAGEGDAEAAVVSVRVAKAEVGPIAQPLDIVGTITGRQEATISPKVSAQIAQMGLIKN
                     RVVHRGDILAVLESRDLNAQRAEASAALREAEVGITNSEQGAIPLSNAQDAKAVHDAQ
                     GTVDNMRKTYERRKTLFEQGGISKKDLEASQLDLAKAEDDLRLAERAAALHKGTTNPS
                     DLAVAKSKVQQASGRLAALNAQADYATIRAPFDGVVTDQFLRQGDFATAGTKLLTIAD
                     LSTVIVKAPLSIDAAANVHAGDVASVMPDSLPGVTLQGTVSLVGGAADPQSRAAEVWI
                     TLPNPDGRLRDNSAARVTINSGNVPNAVIVPTSAVTLDATNANSGTVMVVDAKSVAHE
                     TKVTTGAHTRERTQITSGLRGGETVVIEGNYGLPDGTKVQNAGAAPKTATNAPAEKD"
     gene            complement(2343..3590)
                     /locus_tag="VNN25_21535"
     CDS             complement(2343..3590)
                     /locus_tag="VNN25_21535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH94173.1"
                     /translation="MSALPLAAQTPPPPLTIENATQAALRQATAYQQAVIDEEIAALD
                     LTQARAALLPRARDSFTATYNKPIRPGTTDPAFIAQNSVREYQQLLGVEGSLDFGMRA
                     AMAKSRALLAAAHAGTEIARRALVRGVREAYFGLALATAKRRSAEETLAAAEEFERVN
                     ALQQNAGELAEIDVIRARLQTAQRRDDVEQARLQESNALAAMRVLAGIRAPEPLSVSD
                     LTPQPSSADVERFTTDTIAKRPELTQAEQQLRAARSDIGVARAGRLPALSYSVDEGFD
                     SPSLHASDIREHRGYLAMANLNIPIFDWGIGRAKQRQAELHAQAAENQLAITQRAIEL
                     EFGAAHDEALAAVRRADNARAAVADAQRNADISIARYRAGEAPIFEVTDALTTLAQQR
                     NNYQQALFDFEMARARLQEAAGE"
     gene            complement(3718..4206)
                     /locus_tag="VNN25_21540"
     CDS             complement(3718..4206)
                     /locus_tag="VNN25_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94174.1"
                     /translation="MNARRSTVAVILFLVAIAAGAAEPSPVTTVSFFVSARGMDRSRN
                     LSEGDLRRIFLGEISRWPNGHRIVLYVRPFDSAAGRVFLDRLIRMSDIDYAQWWLGAV
                     FRGRAAAAPRVMATPDSMSKAVAANADAIGFDSQTPDAELLVLTIDGRAPSDPRYAIR
                     AR"
     gene            complement(4211..5314)
                     /locus_tag="VNN25_21545"
     CDS             complement(4211..5314)
                     /locus_tag="VNN25_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94175.1"
                     /translation="MIPCKQRIALVIAMSAALVLVAPPGRGDDASSPEQHFPAFRLGA
                     FADIVLAHPDKQGKVDYESGELDVYTSAQLNNNWSVLGEGLVQHAGSSENPDLTPKSD
                     LQFNLERLYVAYEASDRLRVEFGQIHTGIVQWNEREHRSRFLQTPIDVPSIANRESQG
                     GAWPLHFTGAWASGSVPGSLGATYGAGLGAARGSERDDIQPFYRRGTSPAALFSVGVA
                     PDSLTGFEAGAAGYAGRIRVQGQKTLRELDSTVYSSFVRGGIELRGEWAHMVHSEVGG
                     DAAFVTRGWYLLGSWRPPGRWKPFRPYLFIDHLHVAAGEQYLSDVHNQNAWAAGVRWD
                     VNARLALKGDFRTQLFASPHREGLFRLEVALAF"
     gene            5583..6986
                     /locus_tag="VNN25_21550"
     CDS             5583..6986
                     /locus_tag="VNN25_21550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94176.1"
                     /translation="MLHSVRARLTMWYTAILALVLVIFGAVSYVLLAREIRAETDASL
                     ADTAHEFAAAFDPAEHTQGRDALLDFRYSDREILVLSPRAEVVMASKSKIDAGERKRI
                     EAFVRGGGRGLQTIPGGEEGDGVRIIAMPIDVLGTHYTVVVARALSEQADRLESAAHA
                     VFFGIPVALLVAAAGGYLLARKSLAPVTVMSMKARQIGAETLDERIETGNERDELGFL
                     AVTLNGLLERLQGSFESQRRFMADASHELRTPIAIIQGEADVALGRRDRSAEDYRESI
                     EVMQRAARKLTRIVQNLFLLARGDAGRYPMSMSRFYLGEIVADCVRGMRSVAQSRGVT
                     ITCSAADDMVIVADEELIHRLVLNLVENAVKFTPEGGKVHVSLASEGGSYAIRVADTG
                     PGIPPSEQERIFERFYRGDRVRPTNAGGAGLGLPIARWIAEVHGGELRLDHSGAEGSE
                     FVAVLPSGCHEDLVANG"
     gene            7019..7429
                     /locus_tag="VNN25_21555"
     CDS             7019..7429
                     /locus_tag="VNN25_21555"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH94177.1"
                     /translation="MAIIPTVRCRNMRKSLAFYTGVLDFERVDGDDDMDDPSYSLLSR
                     GGDQLILSSHRGDGAFGQAIVITTDNIDALFRTLRERGLHTPGNPDAPLHVHEGPIDQ
                     SWGTRELYVDDPDGNTLRFTQAEQRPFGSAETRD"
     gene            7564..8253
                     /locus_tag="VNN25_21560"
     CDS             7564..8253
                     /locus_tag="VNN25_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH94178.1"
                     /translation="MRILIVEDDPDMSRFIARGLSEQSYAVDVAATGEAALEAAATSS
                     YDAIILDAMIPPPDGFEVCRRLRAEGIDATILMLTARDSLADRVEGLDSGADDYLVKP
                     FEFAELLARLRALLRRRGTRQYPVIEVGDLRLDTRSHRVTRAGVELQLTTKEYALLEY
                     LSVNAGRVMGREEIAEHVWNDEFDPFSNLIEVYIGRLRRLVDRERQTKLIHTIRGSGY
                     ILEPREPHTAP"
     gene            8321..9010
                     /locus_tag="VNN25_21565"
     CDS             8321..9010
                     /locus_tag="VNN25_21565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HXH94179.1"
                     /translation="MLFIIDFDGTVAPTDTVDDLLEHFADPDWRRIEQQWVNGEINSR
                     QCMAEQIALVSTDEPTLRRFLSSVAIDPAFPEFVDYASTFADLAIVSDGLDFPIQQAL
                     RRAGLPELPVFANHVELRQSGLGLTFPYYEKTCTVGSGVCKCSVARAVDAGRELTTVL
                     IGDGRSDQCVAHQARYVFAKGSLITYCRETELPYIPFQSFADVLATIKGWDILPYQRR
                     LKERQCPLLEA"
     gene            8989..10407
                     /locus_tag="VNN25_21570"
     CDS             8989..10407
                     /locus_tag="VNN25_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH94180.1"
                     /translation="MSVVGSIDTDESLLAVSPDTAELLEKDSRYCSYGDTVHYAVPPK
                     IFQRCDGSYLFDEEDTPYLDLQMWYSAVNFGYKNSRLNDALKKQIDTLPQLACQYLHA
                     EKVELAEALCNVIEKRTGVKGRVHFNVGGAQAVEDSLKLVRNATGKSLNFAFMGGYHG
                     RTLGASAITSSYRYRRRFGHFSDRAQFIPFPYCFRCPYDKKVEDCGLYCVKQFEKLFD
                     TEYHSVWDSKAQQSEFAAFYIEPIQGTGGYVIPPKGYFEELTRILHERGILIVVDEIQ
                     MGIHRTGKFWSFEHFGLQPDIVVFGKALTNGLNPLSGVWAREELISPQVFPPGSTHST
                     FSSNTLGTATALEVMHMIAESDYEGDVNAKGAYFLARLQELKSRWRMIGDVDGIGLAL
                     RIEMCQEDGFAPNQKLTNAVFEEGLKGDISARGRKYGLVLDVGGYYKNVFTLAPSLHI
                     TEDEIDLGLELLESLLQRCGAC"
     gene            10425..11297
                     /locus_tag="VNN25_21575"
     CDS             10425..11297
                     /locus_tag="VNN25_21575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH94181.1"
                     /translation="MYPNQHGAREQHGKAGVLLVHGITGAPAEMKPLARKLTASGFTV
                     SCPQLAGHCSSLTSLKQTQWRDWYRSLETALFTLRDQCDTVFVSGLSMGALLALRLAA
                     RHPELISGVATLSATFFYDGWNVPRLRQKYLLPLLIYSPLRFFLSYHEPSPYGIKDDR
                     IRNVIDAVYQSKTRAMPEKWGYSEFPGVTIRETFRLIKAVKRDLPVITAPLLIVHATE
                     DDMASLENARFLASEVSSSSVETFYVDDTYHVLTLDKRRDDVAARVAEFFTRLQASPP
                     LSGEAVGGLYAQPA"
     gene            11310..11585
                     /locus_tag="VNN25_21580"
     CDS             11310..11585
                     /locus_tag="VNN25_21580"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXH94182.1"
                     /translation="MPTPFERVQAILEKKYSLAPETITPESTLESLGLDSLDLIELLF
                     DVEDEFKIRVPQDGGAALKTTTIQDIIDSVQKMLADTAAQAGAEPAA"
     gene            11582..12814
                     /locus_tag="VNN25_21585"
     CDS             11582..12814
                     /locus_tag="VNN25_21585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXH94183.1"
                     /translation="MRRVVVTGLGVISPLGNDPETLFSRLMEGESGIREMITEGPNGP
                     SSNVAGAAAFNPELYFPAGQKTDHIDRVGLFAMAASGMAVRDSGIAFTDELRERSGVS
                     FGTGMGSAATLEETFAQLLLRDPDRVKPLTVLKVMNNAPGAHVAIQHSLAGPDLTYSC
                     ACSSSSVSIGEAFRQIQYGTADVMIAGGAEAALTFGFFKAWNAMHILAVADREDPSAS
                     CRPFSRNRTGLVLGEGAGMVVLEERDRAIARGARIYAELAGYGSNNDYRHLTKPSVEG
                     QAKAMQLALSDAGVAPEDVSYINAHGTATALNDLTETRAIKSVFGDGASRIPVSSTKS
                     MHGHLLGGAGALEFVISLLALSHNAVPPTANLNVPDPECDLDYVPGRGRSGQDIRCVM
                     SNSFAFGGTNAVLIARRD"
     gene            12836..13192
                     /locus_tag="VNN25_21590"
     CDS             12836..13192
                     /locus_tag="VNN25_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXH94184.1"
                     /translation="MSRLAGALWVFNIFLDTIGHLSFKAAASTKVTAEQNRWRRMFGS
                     VYLWIGIACFCLEFAVWFALLSLIPLSQAVLIGSINIVVVMLAGRLLFRERLDPLRLT
                     GMALIAAGVALAGGTL"
     gene            13189..13575
                     /locus_tag="VNN25_21595"
     CDS             13189..13575
                     /locus_tag="VNN25_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXH94185.1"
                     /translation="MSRGRVVLFGFCALVLIDTWTQVSFKLASRQTGEFVMNTHWFGA
                     AILSPWIYAAVAGYLGAFFTWMTLLEHAPVGPAFAASHLEVVTVLLISIPLFGERVSV
                     AKAAGAVCIVAGIILLSISESKQSHA"
     gene            13568..14794
                     /locus_tag="VNN25_21600"
     CDS             13568..14794
                     /locus_tag="VNN25_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXH94186.1"
                     /translation="MPDRWREVPPTAGLPLYWRDFIGRAASASLEERLAGWLGVPWVR
                     VECSGTSALVIALTTLARASTRRKVIIPGYTCPLVALAVLHCGLVPVVCDVAPSTFDY
                     CPEELARLCDRETLAVIATHLGGRVADLETISAVARREGAFVIEDAAQSLGGQWKGRR
                     AGTVGDIGFFSLAAGKGLTLYEGGVLVARDPEVRRELQATSERIAPYRLLWEVRRSLE
                     LIAYGISYRAGLLPIVYGIALRRALRRGRLVAAVGDEFSSAIPIHRVGAFRRAVGARA
                     AVRLPAFLDDLSMQADARKKTLESVTGITVIGDPEGSQGTWPFFMVLMPSASTRDRAL
                     DRLWRAGLGVTRLFIHALPDYQYLSRQLGACEIPNARDLAARMLTISNSPWLRERDFE
                     RICAQLAGVLRDSSQH"
     gene            complement(14723..15634)
                     /locus_tag="VNN25_21605"
     CDS             complement(14723..15634)
                     /locus_tag="VNN25_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94187.1"
                     /translation="MTRPLILDFDGSVASSDGTVVDLRERQEAIRFGCRFAALRRLGD
                     ELAPALSAMPPTAFLGSGDYHHVSYLLIERLRRLAAPVQVVVFDNHPDNMRYPFGIHC
                     GSWVSHVSRLPFVSCVHVVGITSTDVEGFNVWQNHLLPLHSGRVRYWCVGKDLRGMRR
                     LGIKRSHSFATVGDLLDQFENESRTWRQPVYLSIDKDVLSRDVVQTNWDQGVMTLEEL
                     DLAIGMLKKLVIASDVTGEVSTYRFTTRYKRILAALDHQPEIPEGSLPLWQAQHQAVN
                     RRLLSMLAGVSEHAGQLGADALEIALP"
     gene            complement(15631..16704)
                     /locus_tag="VNN25_21610"
     CDS             complement(15631..16704)
                     /locus_tag="VNN25_21610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH94188.1"
                     /translation="MFADVSEPAFDNLIEPFGLIGHFLEHPPAGFSILDLGNHVPAFS
                     APFDLLTTVERPVRRVIDALPFAGALRRLLVANTCFVGTTTSEYSIFPANTSAADLAN
                     RLATGIAPHYPFLIIKDLPGDALLVGEAAHAWSQELIEACRSHGFVAVEGQALAYVPI
                     DFASTDEFLARRSHARRKNLRRKLKAAAGLRIEEKQSGDDAFYSEPLPARLYELYRNV
                     YDQSEIHFDLLTESFFRSVLQDATIGGIIFLYYDGVELIGFNLCFHEKSMLIDKYIGL
                     AYPQARDHNLYAVSWFHNLEYALAHGLRYYVAGWTDPEVKRELGAQFTFTKHMVYVRN
                     PFLRGLLRRLRRLFESDHARHGG"
     gene            complement(16725..17063)
                     /locus_tag="VNN25_21615"
     CDS             complement(16725..17063)
                     /locus_tag="VNN25_21615"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXH94189.1"
                     /translation="MYKKIITVFAFATILSAGAFAATSTTKQQPKITMEQARATALKK
                     APGKVTSAELENEKGKLIYSFDIATSKTEVTEVNVDAMNGKIVAVQHENAAKEAAEKK
                     QEAKEKTNKQ"
     gene            17189..18013
                     /locus_tag="VNN25_21620"
     CDS             17189..18013
                     /locus_tag="VNN25_21620"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH94190.1"
                     /translation="MRPLLHFVLIILLVTPLAYGDDACGGKVGCAATAVKHEVVRYVR
                     DAKSIATAPLHWDRQAWERFGEGTAAVVALYAVDKQTSNQFAQQRSSSTNQFAKTVTP
                     FGGHRALEISALMILTGAGIHDDTLRDAGRDSLQSEFFAAGIVTPLLKSAFGRARPIQ
                     NEGSQSFHPFDTHFESFPSGHATNAFAFATAVAGHYDGWIVPTIVYTVASGVAVSRVN
                     DHVHFPSDVVAGALIGHAVAKGILARHTGKHVAWQATPLIDRKTMGMMFTIGPARR"
BASE COUNT         3374 a         5896 c         5720 g         3571 t
ORIGIN      
        1 ccgacgacac cgatccgctg accagcagcg actggtcgta gtaaggcttg atctcgacgt
       61 cgcgcggcaa ctgtctacgc agaccttcca gctccgcctt cacgccgcca gccaccgaaa
      121 gaatgttcgc ggtcggctgc cgcatcacgt tcagcagcac agccgggcgt ccatctgccg
      181 tgacgatggt gaagcgcggc tcgagtccgg ggctgacggt ggcgacgtcg ccgacgcgca
      241 ccggcacgtt gttgaccgtc gtcacgacga tcgcggcaag gtcatcgagg gaagtggcct
      301 gtccgctgac gagtgccagc tcgagttgat gattctcgtt gatgaggccc ggcgaattga
      361 tcacgttcga gccgcgcacc gcatccgaaa cctgttgcag cgagacgccg cgcgcttcga
      421 gccgcgagcg gtcgatgagc acgtggtact cgcgcaactg cccgccaagc acctccacgc
      481 tggccacgcc gtcgatccgc gcgatcggcg gacggagcgt gacttctgcg aggttgcgca
      541 acgttcccgg atcgcgcaca ggcgacgtga tgctgtagcc aatgatcggg aacaccgcaa
      601 acgtgaggcg ctccgtccgc tcgatcgttg ccccaggcgg cagcttcgac gcgagctgcg
      661 atatgcgcgt ctgcaccagt tgcagcgtct gcacgatgtc ggtcttccag tcgaagaaga
      721 gatcgacctc ggcggagccc cgcgcggttg tcgacttgat gcgcgcgatc cccggaatgc
      781 cgctcatcgc ctcttcgatc ggtttggtca ccgagacgag cgtttgctgc gcgggcgcga
      841 cgccgttatc ggcgacgatg atgacgcgtg ggaaatccgt ctgcggaaac agcgcgcgcg
      901 gaatgcgggc cagcgcgacg aggccggcga tcgacagcag cccgatcacg agcaataccg
      961 cccgcttctg gctggagatg acgcgcccga ggttcatcag tccttttccg ccggagcgtt
     1021 ggtggcggtc ttcggggccg cgccggcgtt ctggactttc gtaccgtccg gcagaccgta
     1081 gttgccttcg atcacgacgg tctcgccgcc gcgcaggccg gaggtgatct gcgttcgttc
     1141 acgcgtgtgc gcgccggtcg tgaccttcgt ttcgtgcgcg accgactttg catcgacgac
     1201 catgaccgtg ccgctgtttg cgttcgtggc gtcgagcgtg acggcggacg tcgggacgat
     1261 gaccgcgttc ggaacgttgc cgctgttgat ggtgacacgc gccgcgctat tgtcgcgaag
     1321 gcgtccgtcg ggattcggca gcgtgatcca cacctcggcc gctcggctct gcggatcggc
     1381 agcgccgccg acgagactga cggttccctg caatgtcaca cccggcaacg agtcgggcat
     1441 cacgctggcg acgtcgccgg cgtgaacgtt ggcggcagca tcgatcgaca gcggcgcttt
     1501 gacgatgacc gtcgacaggt cggcgatcgt gagcagcttg gtccccgcag ttgcgaagtc
     1561 accctggcgg agaaactggt cggtgacgac gccgtcgaac ggcgcgcgga tcgtggcgta
     1621 gtccgcctgc gcgtttagcg ccgcgagccg gcccgacgcc tgctggacct tacttttcgc
     1681 aaccgcgagg tcggacggat tcgtcgttcc cttgtgcaac gccgccgcgc gctcggcgag
     1741 gcgcaggtcg tcctccgctt tcgccagatc gagctgcgac gcttcgagat ccttcttcga
     1801 gatgccgccc tgctcgaaaa gcgttttgcg gcgctcgtag gtcttgcgca tgttgtcgac
     1861 ggtgccctgc gcgtcgtgaa ccgccttcgc gtcctgtgcg ttgctcagcg gaatcgcccc
     1921 ctgctccgag ttggtgatgc cgacttcggc ctcgcgaagc gccgccgatg cttccgcgcg
     1981 ttgcgcgttc aggtcgcgcg actcgagcac cgcgaggatg tcgccgcgat ggacgacgcg
     2041 attcttgatc aagcccatct gggcgatctg ggcgctgact ttcggcgaga tcgtcgcttc
     2101 ctgtcgcccg gtgatcgtgc cgacgatgtc gagtggttgc gcaatcggcc cgacttccgc
     2161 cttcgccacg cgcacgctga cgaccgccgc ctcggcgtca ccttccccgg cagcgttgtg
     2221 gcgatgccag caccatccgg cgacgatcag gatcaagagc ccgatggcgg cgccaatctg
     2281 cacgcggcgt cgccgccaca gcggctcctc atctgaaccc ggagcgttca tcaatgtgga
     2341 gctcattctc ctgcggcctc ctgaaggcgg gcacgggcca tctcgaaatc gaacagcgcc
     2401 tgctgatagt tgtttcgttg ctgcgccagc gtcgtcagcg cgtcggtgac ttcgaaaatc
     2461 ggcgcttcgc cagcacgata gcgggcgatg gaaatgtcgg cgttgcgctg cgcgtcggcg
     2521 acggcggcgc gcgcgttgtc cgcgcggcgt acggcggcga gcgcttcgtc gtgcgcggcg
     2581 ccaaactcca actcgatcgc gcgctgcgtg atcgcgagct ggttctccgc ggcctgcgcg
     2641 tgcaactccg cctggcgctg cttggcgcgg ccgattcccc agtcgaagat cgggatgttc
     2701 aggttggcca tcgccaggta gccgcggtgc tcgcggatgt cggacgcatg aagcgacggc
     2761 gagtcgaaac cttcatccac cgagtagctg agcgccggaa gtctgccggc gcgggcgaca
     2821 ccgatgtcgc ttcgcgccgc gcgcaactgc tgttcggcct gggtcagttc gggccgcttc
     2881 gcgatcgtgt ccgtcgtgaa gcgctcgaca tccgcgctcg aaggttgcgg cgttagatcg
     2941 gacaccgaca gcggctcggg cgcacgaatg ccggccagga cgcgcattgc cgcaagtgcg
     3001 ttcgattcct gcagacgcgc ctgctccacg tcgtcgcgac gctgtgctgt ttgcagccgt
     3061 gcgcgaatca catcgatctc ggcgagctct cctgcattct gctgcaacgc attgacgcgc
     3121 tcgaactcct cggcggcggc cagcgtctcc tccgcgctgc ggcgcttggc agttgcgaga
     3181 gcaagcccga agtaggcctc gcgcacgccg cgtacgagcg cacgccgggc gatctcggtt
     3241 cctgcgtgtg ccgcggcgag cagcgcgcgg ctctttgcca tcgccgcacg catcccgaag
     3301 tcgagcgagc cttcgacgcc aagaagctgc tggtattcac gcaccgagtt ctgggcaatg
     3361 aacgccggat cggtcgtgcc gggacggatc ggtttgttgt acgtggccgt gaacgagtcg
     3421 cgcgcgcgcg gcaggagcgc ggcgcgtgcc tgcgtgagat cgagtgccgc gatctcctca
     3481 tcgatgaccg cctgctgata ggcggtggcc tgccgcagcg ccgcctgcgt ggcgttctcg
     3541 atcgtgagcg gaggcggcgg cgtctgcgcg gcgagaggca gagctgacaa caaggaaatc
     3601 gtgagcagac agggcctgaa catcaagcgc cggagtttaa ccgaaccaag atgaaccaca
     3661 gagacacgga gaacacagag gtttctctgt gtcctttgtg tctctgtggt gcaagcctta
     3721 acgcgcccga atcgcatacc gcggatcact cggcgcacgc ccatcgatcg tgagtaccag
     3781 caactcagcg tcgggcgttt gtgaatcaaa cccgatcgcg tcggcattcg ctgccacagc
     3841 cttcgacatc gagtccggag tcgccatcac gcgcggcgcc gcggcggccc tcccgcggaa
     3901 gacggcaccg agccaccatt gcgcgtagtc gatgtccgac atgcggatga gccggtcgag
     3961 gaacaccctt cccgcggcag aatcgaacgg acggacgtag agaacgatgc gatgcccatt
     4021 cggccaccgg ctgatctcgc cgagaaagat ccgccgcaga tcgccctcgg acagattgcg
     4081 actgcggtcc atgccgcgcg ccgagacgaa aaacgaaacc gtcgtcaccg gcgatggctc
     4141 cgccgctccc gccgcgattg cgacaagaaa caggatgacg gcgacggtcg accggcgagc
     4201 gttcatcgcg tcaaaacgcc agcgccacct cgaggcggaa aagaccttcg cgatggggcg
     4261 acgcaaagag ctgcgtgcgg aaatccccct tgagtgccag ccgcgcgttg acgtcccatc
     4321 gcacgccggc cgcccaggcg ttctggttgt gaacatcgct caggtactgc tcacccgccg
     4381 caacgtgcag atgatcgatg aagagatacg ggcggaacgg cttccaacgg ccgggcggac
     4441 gccacgatcc gagcaggtac cagccgcgcg tcacgaacgc agcgtctccg ccaacttccg
     4501 agtggaccat gtgtgcccac tcgccgcgca gctcaatgcc gccgcgaacg aatgacgaat
     4561 aaaccgtgga atccaattca cgcaacgtct tctgcccctg cacgcgaatc cgtcccgcgt
     4621 aaccagcggc gcccgcttcg aatcctgtca gcgagtcggg cgcaacgccg accgagaaga
     4681 gcgcagccgg cgaggtgccg cggcgataga acggctgaat gtcgtcccgc tcgctgccgc
     4741 gtgccgcgcc gaggccggca ccgtacgtcg cgccaagcga gcccggcacg ctgccggacg
     4801 cccacgcacc cgtgaagtgg agcggccagg cgccgccctg cgactcgcgg ttggcgatcg
     4861 aaggaacatc gatcggcgtt tgcaggaacc ggctgcgatg ctcgcgctcg ttccactgca
     4921 cgatgccggt gtggatctgg ccgaactcga cgcgcaggcg gtcggacgcc tcgtacgcga
     4981 cgtacagccg ctcgagattg aattgaagat ccgatttcgg cgtgaggtct ggattctcgg
     5041 agcttcctgc atgctgcacg agtccttcgc cgagcacgga ccagttgttg ttgagttgcg
     5101 ctgaggtgta gacatcgagc tcgcccgact cgtagtcgac cttcccttgc ttgtccggat
     5161 gggcgaggac gatgtcggca aaggcaccga gtcggaatgc ggggaagtgc tgctcaggcg
     5221 aggaagcgtc gtcgccgcgg ccgggtggcg cgacgagcac gagcgctgca gacatcgcga
     5281 tgacgagggc gatccgttgc ttgcacggga tcatggcgtt tgaatctacc gcaatcatga
     5341 ctcaacgcta cgaacggcga cttaaacgcg ggatgaagcg ggcggttcat cggcagttca
     5401 cctcggcgcg ctcacgcttg cctgacttcc ggcggccacg tttgcgatca tctatctctt
     5461 cacaaccgat tttcgtctca cgcaacgagg agcatcacaa tcccgtcaag ccgcgcgata
     5521 cgcagactcg gggattcgat gagactggac ggcaacgcgc ccatggcaca acactaccgt
     5581 ggatgctgca ctcggttcgc gcgcgcctga cgatgtggta caccgccatt ctggcgctgg
     5641 tgctcgtgat tttcggcgcc gtgagctacg tgctgctggc gcgcgagatc cgtgccgaga
     5701 ccgacgcgtc cctcgccgac accgcgcacg aattcgcggc ggcgttcgat ccggccgagc
     5761 acacgcaggg gcgcgatgcg ctgctcgact ttcgctacag cgaccgcgag attctcgtcc
     5821 tgtcgccgcg cgccgaggtc gtcatggcgt cgaaatcgaa aatcgatgcc ggcgaacgaa
     5881 agcggatcga ggcgttcgtt cgtggcggtg gccgcggttt gcagaccatc cccggaggcg
     5941 aggaaggtga cggcgttcgc atcattgcca tgcccatcga cgtcctcggc acgcactaca
     6001 ccgtcgtcgt cgcgcgtgcc ctcagcgaac aagctgatcg cctggagagc gcggcgcatg
     6061 cggtgttctt cggcatcccc gtcgcgctcc tcgtggccgc cgcgggcggt tatctcctgg
     6121 cccgcaagag cctcgcaccc gtgaccgtca tgagcatgaa agcacgtcag atcggtgccg
     6181 agacgctcga cgagcgcatc gagaccggca acgagcgcga cgagcttggg tttctggccg
     6241 tcacgttgaa cggacttctc gagcgtctgc aggggtcgtt cgagtctcaa cgacgcttca
     6301 tggccgatgc ctcgcacgaa ttgcgcacac cgatcgcgat cattcaaggc gaggccgatg
     6361 tcgccctcgg acgccgcgat cgttccgccg aggactatcg cgagagcatc gaggtgatgc
     6421 agcgcgcggc caggaaactc acccgcatcg tgcagaatct gtttcttctg gctcgcggtg
     6481 acgcaggccg ctatccgatg tcgatgtcgc gcttttacct cggcgagatc gtcgccgatt
     6541 gcgttcgcgg catgcgtagc gtcgcacagt cgcgcggcgt gacgatcacc tgctcagctg
     6601 ccgacgacat ggtcatcgtc gccgacgaag agctgatcca ccggctcgtc ctgaacctcg
     6661 tcgagaacgc ggtgaaattc acgccggagg gaggcaaggt tcacgtgtcg cttgcaagcg
     6721 aaggagggag ttacgcgatc cgcgtggccg acaccggccc cggcatcccc ccttccgagc
     6781 aggagcggat tttcgagcgc ttctatcgtg gcgaccgcgt ccgaccaacc aatgcaggcg
     6841 gcgccggtct cggcctcccg atcgcgcgat ggatcgccga ggttcatggc ggagaattac
     6901 ggctcgatca ttccggagct gagggtagcg agtttgtggc ggtattaccg agtgggtgcc
     6961 atgaagatct ggtggcgaat ggttagcacg ccggtgacaa gataaggtcc agaagacgat
     7021 ggcaattatc ccgaccgttc gttgcagaaa catgcggaag tcgctcgcgt tctataccgg
     7081 tgtgctcgat ttcgagcgtg tggatggcga tgacgacatg gacgacccgt cctacagtct
     7141 tctctcacgg ggcggagacc aactcatcct gtccagccat cgcggagacg gcgcgttcgg
     7201 tcaggcgatc gtcatcacga ccgacaacat cgacgcgctg tttcgcaccc ttcgggagcg
     7261 agggctacac acaccgggga atccggacgc tcccctgcac gtccatgaag gcccgatcga
     7321 ccagagctgg ggcacgcgcg agctctatgt cgacgatccg gatggcaaca cgcttaggtt
     7381 cactcaggcc gagcaacggc ccttcggatc ggcagagact agagactaga gactagagac
     7441 tagagactag agacaagaga caaggaagca agagcagaga taggaggtca gagataagga
     7501 gtcaagcgtc atagagaatc cccgatctct gatctctgat gtctctgatc tctgatttac
     7561 accatgcgca ttctcatagt cgaagatgac ccggacatgt cgcgtttcat cgcgcgcggc
     7621 ctcagcgagc aatcctacgc agtcgatgtc gcggcgaccg gcgaggcggc gctcgaagcg
     7681 gcggccacct cctcgtacga cgcgatcatt ctcgatgcca tgatcccgcc gccggatggt
     7741 ttcgaagtct gccgcaggct gcgcgccgag ggaatcgatg caacgatcct gatgctcacg
     7801 gcgcgggact cgttggccga tcgcgtcgaa ggcctcgact ccggcgccga cgactatcta
     7861 gtcaagccct tcgagttcgc cgagctgctg gcccgtctgc gcgccttgct gcgccgccgc
     7921 ggaacccgcc agtatccggt catcgaagtc ggtgacctcc ggctcgatac ccgctcccac
     7981 cgcgtcaccc gcgccggagt cgagctgcaa ctgacgacga aggagtacgc gctcctcgaa
     8041 tacctctccg tcaacgcggg acgtgtcatg gggcgggaag agatcgccga gcacgtctgg
     8101 aacgatgagt tcgacccatt ctcgaacctc atcgaggtct acatcggccg cctgcggcgt
     8161 ctggtcgacc gcgagcgcca aacgaaactg atccatacga tccgaggatc gggctacatc
     8221 ctcgagccgc gcgagccgca cacagcgccg tgacgcgatt caccgctgat ttatccgtgg
     8281 ccgtgcacca tgtccttcct ggtccgggca ccacacactc atgttattca tcatcgactt
     8341 tgacgggact gtcgccccta ccgataccgt tgacgacctg ctcgagcact tcgccgatcc
     8401 tgattggcgg cgcatcgagc aacagtgggt caacggcgag atcaactcgc gccagtgcat
     8461 ggccgaacag atcgcactgg tatcgaccga cgagccaacg ctgcggcgct ttctctcctc
     8521 cgtagccatc gatcccgcct ttcccgaatt tgtcgattac gcctcgacct tcgccgatct
     8581 ggccatcgtc agcgacggcc tcgatttccc gattcaacag gctctgcgcc gggccggcct
     8641 gcctgagctt ccggtcttcg cgaaccacgt cgagctccgc cagtccggcc tcgggctcac
     8701 atttccttac tatgaaaaaa catgtacggt gggcagcggc gtgtgcaaat gcagcgtcgc
     8761 acgcgcggtc gacgccgggc gcgagctgac cacggtgctc atcggcgacg ggcgatccga
     8821 tcagtgcgtc gcccatcagg cgcgctatgt cttcgccaag ggatcgctga tcacctactg
     8881 ccgcgagacg gaactccctt acatcccatt ccaatcattt gccgatgtcc tggccacgat
     8941 caagggatgg gacatcctgc cgtatcaaag acgtttgaag gagaggcaat gtccgttgtt
     9001 ggaagcatag ataccgacga atcacttctt gcagtttcgc ccgacacggc ggaactgctc
     9061 gaaaaggaca gccgttattg ttcgtacggc gacaccgttc attacgcggt acctccgaag
     9121 atctttcaac gctgcgacgg cagctatctc ttcgacgaag aagacacgcc atatctcgat
     9181 ctgcagatgt ggtactccgc ggtcaacttc ggctacaaga atagccgtct caacgacgcc
     9241 ctgaagaaac agatcgacac gctcccccaa ctggcctgtc agtatctgca cgccgagaaa
     9301 gtcgagcttg cggaagcgct ctgcaacgtg atcgagaagc ggaccggagt gaaagggcgc
     9361 gtccacttca acgtcggcgg cgcccaggcg gtggaggatt ctctcaagct ggtgcgcaat
     9421 gcgaccggga agagcctcaa tttcgcgttc atgggcggct accacggccg caccctcggc
     9481 gcgtccgcca tcacgtccag ttatcgctac cgccgccgct tcggccactt ctccgaccgc
     9541 gcacaattca tccccttccc ctactgcttt cgctgtccgt atgacaagaa ggttgaggac
     9601 tgcggcctct actgtgtgaa gcagttcgag aagctcttcg acacggagta ccactccgtg
     9661 tgggacagca aggcgcaaca atcagaattc gcggcgttct acatcgagcc gattcaggga
     9721 acaggtggat acgtcatccc gccgaaaggc tatttcgagg aattgacgcg tattctgcac
     9781 gagcgcggca tcctgatcgt ggtcgacgag atccagatgg ggattcaccg caccggaaag
     9841 ttctggtcct tcgagcactt cggccttcag cccgatatcg tcgttttcgg gaaagcgctg
     9901 acgaatggac tgaacccgct gtccggcgtc tgggcgcgcg aggagctgat ctcgccgcag
     9961 gttttcccgc cgggctcgac ccactcgacc ttctcctcga acaccctcgg caccgccacc
    10021 gcgctcgagg tcatgcacat gatcgcggag tccgactacg aaggcgacgt gaatgccaaa
    10081 ggggcctact ttctggcccg cctccaggag ctgaaatcgc gttggcgtat gatcggcgac
    10141 gtcgatggaa tcggtctcgc tctccgtatc gagatgtgtc aggaagatgg cttcgctccc
    10201 aatcagaagc tcacaaacgc ggtgtttgaa gaaggcttga aaggggacat ctccgcacga
    10261 gggcgcaagt atggtctggt cctcgatgta ggcggttact acaagaacgt gttcacgctc
    10321 gctccttcac tccacatcac cgaagacgag atcgacctag gcctggaact gctcgaatca
    10381 ttgctgcaac gttgcggggc ctgttgaggc ctgtgaacct gaccatgtat ccaaaccagc
    10441 acggagctcg cgaacagcac ggaaaagcag gtgtcctgct cgtccacgga atcaccggtg
    10501 ctcccgcgga gatgaagccg ctcgctcgaa agctcacggc cagcggcttc acggtgtcct
    10561 gcccgcaact ggcgggccat tgctcgtctc tcacatcgct caagcagacc cagtggcgcg
    10621 attggtatcg ctcgctggag actgcgctct tcactctgcg cgatcagtgc gacaccgtct
    10681 tcgtctcagg gctgtcgatg ggcgctctgc tcgcactgcg gcttgcggcc aggcatccgg
    10741 agttgatttc gggcgtggca actctctctg caacattctt ctatgacggc tggaacgtgc
    10801 cgcgccttcg tcagaagtac ctgttgccgc tgctcatcta ctcgccgctc cgcttcttcc
    10861 tgtcgtacca cgagccttcg ccttacggca tcaaggatga ccgaatccgc aacgtgatcg
    10921 acgccgtcta ccagagcaaa acccgagcga tgccggagaa gtggggatac tcggaattcc
    10981 ccggcgtgac catccgcgag acctttcgcc tcatcaaggc agtgaagcga gacttgccgg
    11041 tgatcacggc gccgctgctc atcgttcacg ccacggaaga cgacatggcc agcctcgaga
    11101 acgcgcggtt tctggcgtcg gaagtttcgt cctcttccgt cgagactttc tatgtcgacg
    11161 acacctatca tgtgctgacc ctcgacaaac ggcgggacga cgtcgccgcc cgggtcgcag
    11221 aattcttcac gcgtctgcag gcaagtcccc cgctttcagg cgaagcagta ggcgggttgt
    11281 atgcacagcc cgcttgagga ggtctagtcg tgcccacacc atttgagaga gtgcaggcca
    11341 ttctcgaaaa gaagtattcg ctcgcgcccg aaaccattac cccggagtca acactggaga
    11401 gtctcgggct cgattcgctc gacctcatcg agctgctgtt cgacgtcgaa gatgagttca
    11461 agatccgtgt gccgcaggac ggcggtgcgg cattgaagac gacgacgatc caggacatca
    11521 tcgacagcgt ccagaagatg ctggccgata ccgcagccca ggccggagcg gagccagcgg
    11581 cgtgaggcgt gtggtcgtca ccggcctcgg cgtcatctcg cctctgggca acgaccccga
    11641 aacgcttttc tcgcggctga tggagggaga gtcggggatc cgcgagatga tcacggaagg
    11701 accgaacggc ccgtcctcaa acgtcgctgg tgccgccgcg ttcaatcccg agctttactt
    11761 tccggcaggt cagaagaccg atcacatcga ccgcgtggga ctgttcgcga tggctgcctc
    11821 cggcatggcc gtccgcgata gcggaatcgc gttcaccgac gagctccgcg aacgatccgg
    11881 cgtctcgttc gggaccggga tggggtcggc agcgacactc gaagagacgt tcgcgcagtt
    11941 gctcctgcgc gatccggacc gggtgaaacc gctcacggtt ctcaaagtga tgaacaacgc
    12001 cccgggagca cacgtcgcca tccagcactc tctcgccgga cccgatctga cctactcctg
    12061 cgcctgctcc tcgtcgtccg tttcgatcgg cgaagcattc cggcaaatcc agtacgggac
    12121 cgcagacgtg atgatcgccg ggggagccga agcggcgctg acgtttggct ttttcaaagc
    12181 gtggaatgcg atgcacattc tggcggtggc cgaccgcgag gatccttccg cgtcctgccg
    12241 gccgttctcg cgcaatcgaa caggcctcgt tctgggcgag ggcgccggaa tggttgtgct
    12301 ggaagagcgc gaccgggcca ttgcgcgcgg cgcacgaatc tatgcggagc tcgccggcta
    12361 tggatcgaac aacgattacc gacatctgac gaaaccctcg gttgagggac aggccaaagc
    12421 gatgcagctg gctttatccg atgccggggt tgcgcccgag gatgtcagtt atatcaatgc
    12481 tcacggcact gccacggcac tcaatgacct gaccgagacc cgggcgatca agtccgtgtt
    12541 tggcgacggc gcttccagga ttcccgtcag ctccacgaaa tcgatgcacg gccatcttct
    12601 tggcggcgcc ggtgcattgg agttcgtcat cagcctcctg gcgttgtcgc acaacgcagt
    12661 acctccaacc gcaaatctca acgtccccga tcccgagtgt gacctcgatt acgtgccggg
    12721 gcgagggagg agcgggcagg acatacgatg cgtcatgtcg aactcgttcg cgttcggcgg
    12781 gacgaacgcc gtcctgatcg cacgccgcga ttgaagtccg agggctgaca tcgccatgtc
    12841 gagactggcc ggcgctctct gggtgttcaa catttttctc gacacgatcg gccatctttc
    12901 gttcaaggcg gctgcttcta caaaggtgac ggctgaacag aatcgctggc ggcggatgtt
    12961 cggctccgtt tacctctgga tcggcatcgc ctgcttctgc ctcgagttcg cggtctggtt
    13021 tgcgctcctc tcgctgattc cgctttccca ggcggtcttg atcggctcga tcaacatcgt
    13081 tgtggtcatg ctcgccggca ggctgttgtt ccgcgaacgg ctcgacccgt tgcgtttgac
    13141 gggaatggca ctcattgcgg ccggtgtggc gctcgcgggc ggcacgttat gagccgcggg
    13201 cgcgtggtgc tgttcgggtt ctgcgcgctc gtgttgatcg atacctggac gcaggtctcg
    13261 ttcaagcttg catcgaggca gacgggcgag tttgtgatga acacccattg gttcggtgcc
    13321 gcaatcctca gcccctggat ctatgccgcg gtcgccggat acctcggcgc cttcttcacc
    13381 tggatgacgc tactggagca tgctcccgtt gggccggcgt ttgccgcgtc gcacctcgag
    13441 gtggttacgg tgttgctcat ttcgattccg ctcttcggcg aacgcgtttc ggtggcgaag
    13501 gcagcgggag cggtctgcat tgtggcgggc attatcctgc tcagcatcag tgaatcgaag
    13561 cagagccatg cctgatcgct ggagggaagt tccgcctacg gccggccttc cgctgtattg
    13621 gcgcgatttc atcggacgcg ctgcctcggc ttccctcgaa gagcgcctcg ccggatggct
    13681 tggcgtgccg tgggtcagag tggaatgctc cggaacatcg gcgctcgtga ttgctctgac
    13741 gacgctcgcg cgcgcctcca cgcggcgcaa ggtgatcatc cccggatata cctgtccgct
    13801 ggtggcactg gccgttcttc attgcggatt ggtgcctgtc gtctgcgatg ttgcgccgag
    13861 cacgttcgat tactgtcccg aggagctcgc gcgtctttgt gatcgcgaaa ccctcgctgt
    13921 cattgcgacc catttgggcg gacgggttgc cgatctcgaa accattagcg ctgtcgcgcg
    13981 gcgtgaaggc gccttcgtca ttgaggatgc cgcgcagtcg cttggcgggc aatggaaggg
    14041 tcggcgtgca ggtaccgtcg gcgacatcgg cttcttcagc cttgccgccg gcaagggtct
    14101 cacactttat gagggtggcg tccttgttgc gcgtgatccc gaggtacggc gggagctgca
    14161 ggcgacaagt gagcggattg cgccttatcg attgctttgg gaggtgcgcc ggtcgttgga
    14221 gttgatcgcg tacggcatct cgtatcgagc aggcctgctt ccaatcgtct acgggatagc
    14281 gttgcgaaga gcgttacgca gaggccgatt ggtggctgcc gtcggcgatg agttctcgtc
    14341 tgcaatcccg atccatcgcg tcggcgcgtt ccggcgcgcg gtgggggcac gagccgccgt
    14401 ccgtctgccc gcattcctcg atgatctctc gatgcaagcc gatgctcgca agaagacgct
    14461 cgaatccgtc accggcatca cggtgatcgg agacccggaa ggctctcaag ggacgtggcc
    14521 tttcttcatg gtgctgatgc cgtcggcaag cactcgcgat cgcgcgctcg atcgattgtg
    14581 gcgtgccggc ctcggcgtta ctcgactgtt cattcacgca ctgccggact atcagtatct
    14641 gagccgtcaa ctcggcgcgt gcgagatccc gaacgcgcgc gatctcgcgg ctcgcatgct
    14701 cacgatatcg aacagcccct ggctacggga gcgcgatttc gagcgcatct gcgcccaact
    14761 ggccggcgtg ctccgagact ccagccagca ttgagagcag ccggcgattc acggcctgat
    14821 gctgcgcctg ccaaagtggc aatgagcctt ccggaatctc cggttgatgg tcgagcgcag
    14881 ccagaatccg cttgtagcgc gttgtgaagc gatacgtgga cacttcgccg gtcacgtcgc
    14941 tcgcgatcac gagcttcttc agcatgccga tcgcaagatc gagctcctcc agcgtcatga
    15001 caccctgatc ccagttcgtc tgcacgacgt cgcgggacag cacatccttg tcgatcgaga
    15061 gatagaccgg ctgcctccag gttcgcgatt cattctcgaa ttgatcgagc agatcgccca
    15121 cggtcgcaaa ggagtggctg cgcttgatgc ccaatctgcg catcccacgc aaatcttttc
    15181 ccacgcacca gtaacgcacc cgaccagaat gcagcggcag gagatggttc tgccagacgt
    15241 tgaagccttc aacatccgtc gacgtgattc caactacatg aacgcatgac acgaacggca
    15301 ggcggctgac gtgcgaaacc cacgaaccgc aatgaatgcc aaagggatag cgcatgttgt
    15361 ccggatggtt atcgaaaacg acgacctgca caggggcagc gagacgacgc aggcgctcaa
    15421 tgagcaggta gctgacgtgg tgatagtcgc cgctgccgag gaaggcggtg ggcggcatcg
    15481 cactcagtgc gggtgcaagc tcgtcgccga ggcggcgcaa ggcggcgaag cggcacccga
    15541 agcgaatggc ctcctggcgc tcccgcaggt cgacgacggt accgtccgag gaggcgactg
    15601 agccatcgaa atcgagaatc agcggccggg tcatcctcca tgcctcgcgt gatcgctttc
    15661 gaacagacgt ctcaatcgtc gcagcagacc gcgaagaaac gggtttcgca catagaccat
    15721 gtgcttcgtg aacgtgaact gcgctcccag ttcccgtttc acttccggat cggtccagcc
    15781 ggccacgtag tagcggaggc cgtgcgccag cgcgtactcg agattgtgaa accagctcac
    15841 cgcatagaga ttgtgatcgc gggcctgggg atacgcgagt ccgatgtatt tgtcgatgag
    15901 catcgacttc tcatgaaagc agagattgaa gccgatcagc tcgacgccat cgtaatagag
    15961 aaagatgatc cccccgattg tcgcatcctg caggacggat cggaagaatg actccgtcag
    16021 gagatcgaaa tggatctcgc tctggtcgta gacgttcctg tagagctcat acaagcgcgc
    16081 cggcaacggc tctgagtaaa acgcgtcatc accggattgc ttctcctcga tgcgcagtcc
    16141 cgcggctgcc ttcagtttcc gccgcagatt ctttcgccgg gcatgggagc gtcgcgcgag
    16201 gaattcatca gtcgacgcaa agtcgatcgg gacatacgcg agcgcctgcc cttccacggc
    16261 gacaaagcca tgactgcggc acgcctcaat gagctcctgc gaccatgcgt gcgccgcctc
    16321 acccaccagc agtgcatcgc ccgggagatc cttgatgatc aggaagggat agtgcggtgc
    16381 aataccggtg gcgagacggt tcgcaagatc agcagccgag gtgttggcag ggaagatcga
    16441 gtactccgag gtggtcgtac cgacaaagca ggtattggca acgagtaacc gccgcaaagc
    16501 ccccgcaaac ggcaacgcat cgatcacgcg acgaactggc ctttcgaccg tcgtcagcaa
    16561 atcgaacggc gcagagaagg caggaacgtg gttgccgagg tcgaggatcg agaaaccggc
    16621 gggagggtgc tccagaaagt gtccgatcaa gccaaagggc tcgatcagat tgtcaaaggc
    16681 cggctcgctg acatccgcaa acacgccccg attctgcacc ccattcactg cttgttcgtc
    16741 ttctcttttg cttcctgctt cttctcggcc gcttccttcg cggcgttctc gtgctgcacg
    16801 gccacgattt ttccgttcat ggcatcgacg ttgacttccg tcacctccgt cttcgatgtg
    16861 gcgatgtcga acgagtagat gagcttccct ttctcgttct cgagctctgc gctcgtgacc
    16921 ttgcccggcg ccttcttcag cgccgtggcg cgcgcctgct ccattgtgat cttcggctgc
    16981 tgtttggtgg tcgaggtagc ggcgaaggcc ccagccgaaa ggatcgtggc gaatgcaaat
    17041 acggtaatga tcttcttgta catggttccc cctgttattg gcacaactga catttgccaa
    17101 cgtgtcgagt attcagcgca ttgcataaac ttccgctaaa cattcgcttc atcgaccgtt
    17161 cacctcggag cctgtgtaat cttcacgcat gcggcccctc ctccacttcg ttctcatcat
    17221 tcttctcgtg acgccgctgg cgtatggcga cgatgcgtgc ggcggcaagg tcgggtgtgc
    17281 agccaccgcc gtcaagcatg aggtcgtccg ctacgtgcgc gacgcgaaaa gcattgcgac
    17341 ggcgccgttg cattgggatc gccaggcgtg ggagcgcttc ggcgaaggaa cggcagcggt
    17401 ggtcgcgctg tacgcagtcg acaagcagac atccaaccag ttcgcgcagc agcggtcatc
    17461 atcgaccaat caattcgcga aaacggtgac gccgttcggc ggtcatcgcg cgctcgaaat
    17521 ctcggcactg atgattctga ccggcgccgg tatccacgat gacacgttgc gcgacgccgg
    17581 ccgcgactcg ctgcagtcag agttcttcgc cgcgggaatc gtcacgccgc tgctcaagag
    17641 tgcattcggc cgcgcccggc ccatccagaa cgaaggcagc cagtcgtttc acccgttcga
    17701 cacacacttc gaaagcttcc cctccggcca cgccaccaac gccttcgcct tcgccaccgc
    17761 cgtggccggc cactacgatg gatggatcgt cccgacaatc gtctacacgg tggccagcgg
    17821 cgtcgccgtc tcccgcgtga atgaccacgt gcacttcccc tccgacgtcg tggcgggagc
    17881 actgatcggc catgcagtcg cgaaaggaat cctggcccgg cacacgggaa agcatgtcgc
    17941 ctggcaggcc acgccgctca tcgatcggaa gacgatggga atgatgttca cgatcggccc
    18001 ggcgcggcgc tgaggacttc ggcgacggtt agccctcggt tagcccgcat ttctcacacc
    18061 cttatcccca gttcctccag ctccttccca tcgagcgcct tatcccccgc gctttttgcg
    18121 gggggagaag gcgctcgacg caaaggagtc gctgaaaacg tatgagaaat gcggccagtc
    18181 gagggctaat cgcgacaaac cacagataac cggtacatac cgccgcgtta tcgcgttcat
    18241 ccgccgtgtc gtcgcgtttg accgccgttc cgtcgcgttt gaccgccgtt ccatcgcgtt
    18301 tgatcgccgt ccatcgcgtt tgaccgccgt cccgtcgcgt ttgatcgccg ttccgtcgcg
    18361 tttgaccgcc gtcccgtcgc gtttgaccgc cgttccgtcg cgcttgaacg ccgccccgtc
    18421 gcgcttgacc gccgttccgt cgcgtttgac cgcgttccgt cgcgtttgac cgcggtcccg
    18481 tcgcgcttga acgcggttcc ccgtgcttga acgcggtgtt ctcgtgttta tctgtgaaaa
    18541 atcgcgcctg tccacaggac t
//
LOCUS       DATLVW010000740         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_201129, whole genome shotgun sequence.
ACCESSION   DATLVW010000740 DATLVW010000000
VERSION     DATLVW010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201129"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VNN25_21625"
     CDS             <1..2020
                     /locus_tag="VNN25_21625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXH94191.1"
                     /translation="SSHTAAPVAATTAPPVAAYFAPHGFDLTAMDTSVKACDDFYRFA
                     VGKWRDTHPLPAQYSRYGRFEELSERNRDVLHQIAEQDAALTTAAPGSAEQKVGDFYA
                     SCMNETAIETAGVTPIQPELDRINAVSDRASLLAEITHMQTAGYAPLFRVGGQNDLKN
                     SKMIIAGLGTGSLGLPDRDYYLRDTDNFKKIRVQYVDHVTKMLTLAGVNPDQAASDAQ
                     RILVLETKLATAQLPRVELRSPENTYHPTKLADLASMAPAVDWPSYLHTLGVNETSIN
                     VSQPKYIQSVSQLLNDVPLDDWKALLRWQVVEGAAPSLSSAFVTEDFNFSGRVLSGTK
                     EQQERWKRCVRSADGNIGQLLGQEYIRRNFTPEAKAKMNKLIDNLVGALREDIPTLTW
                     MGPETKAAALAKLNAFQRRIGYPDKWRDYSALAISRASYAANVQASRAWAYHHNIERI
                     GHPDDPSEWGGFTPPTVNASYNPPQNNITFPAGILQPPFFDPNADDAYNYGGIGTVIG
                     HEMTHGFDDQGAKFDPQGNLRNWFTPEDLKAFQARTDCVATEYSEFNVAPGININGKL
                     VNGESVADLGGATIALRAYEKSLEGKERKTIDGFSPEQRFFLGFAQVWGENMAAQEAT
                     RRALTDPHAQGPFRVNGTVQNMPEFWKAYHCNEGDKMVREASKRCSIW"
     gene            complement(2072..>2524)
                     /locus_tag="VNN25_21630"
     CDS             complement(2072..>2524)
                     /locus_tag="VNN25_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HXH94192.1"
                     /translation="ELIDFCELSPELSRDFRGLRVWLPLKLFGIEPFRQQLEEKLDLT
                     EWAAAQLHTINGIEVIAEPQLSITAFRLVHPGLDDAAIDALNHDLIARINARQRVMLT
                     GATVAGRFVIRICVVSHRTHRERMEMCMEDVRAAVSETDPQMSQMNAD"
BASE COUNT          506 a          810 c          776 g          432 t
ORIGIN      
        1 atcgtcacac accgccgcac ccgttgctgc gacaaccgcg ccgccggttg ccgcctactt
       61 cgcgccgcac ggtttcgatc tgactgcgat ggataccagc gtcaaagcct gcgacgactt
      121 ctatcgattc gctgtcggca agtggcgcga cacgcatccg ctcccggcgc agtactcccg
      181 ctacgggcga ttcgaagagc tctcggagcg caaccgcgat gtgctccatc agatcgccga
      241 gcaggatgcg gcgctcacca ccgccgctcc cggcagcgcg gaacagaaag tcggcgactt
      301 ttacgcctcc tgtatgaatg aaaccgcgat tgagactgcc ggcgtcacgc cgatccagcc
      361 ggagctcgat cggatcaacg cagtcagcga ccgggcctcg ctcctcgccg agatcactca
      421 tatgcagacg gcaggctacg cgccgctctt ccgcgtgggc ggtcagaacg atctcaaaaa
      481 cagcaagatg atcatcgccg gcctgggcac gggatcgctc ggacttcccg accgcgacta
      541 ttacctccgc gacaccgaca acttcaagaa gattcgcgtt cagtacgtcg atcacgtcac
      601 caaaatgctc actcttgccg gcgtaaatcc cgatcaggct gcgtcggacg cgcagcggat
      661 cctggtgctg gagacgaagc tggccacggc gcagcttccc cgcgtcgagc tgcgcagtcc
      721 cgagaacacg tatcacccaa ccaagctcgc cgacctcgct tcgatggcgc ctgccgtcga
      781 ctggccctcg taccttcaca cgctcggcgt caacgagact tcgatcaacg tctcgcagcc
      841 gaagtacatc cagtcggtca gccagctcct aaacgacgtt cctctcgatg actggaaggc
      901 tctgttgcgc tggcaggtcg tcgaaggcgc cgcgccgtcg ctgtcgagcg cgttcgtgac
      961 ggaagacttc aactttagcg gccgcgtcct ctctggtacg aaggaacagc aggaacgttg
     1021 gaagcgctgc gtgcgctcgg ccgacggcaa catcggccag cttctcggcc aggagtacat
     1081 ccgccgcaac ttcacaccgg aagcgaaggc gaagatgaac aagttgatcg acaacctcgt
     1141 cggtgccctg cgcgaggata tcccgactct gacgtggatg ggaccggaga cgaaggcggc
     1201 cgcgctggcg aagctgaacg cgttccagcg ccgcatcggc tatcccgaca agtggcgcga
     1261 ttattcggcg ctcgcgatca gccgtgcctc gtacgcggcg aatgtgcagg catcgcgggc
     1321 atgggcgtat caccacaaca tcgagcgcat cggccatccg gacgatccga gcgagtgggg
     1381 tggtttcacg ccgccgacgg tgaacgccag ctacaacccg ccgcagaaca acatcacgtt
     1441 cccggccggt atcctgcagc cgccgttctt cgatccgaat gcggacgatg cctacaacta
     1501 cggcggcatt ggcacggtca taggccacga gatgacgcac ggcttcgacg atcagggggc
     1561 gaagttcgac ccgcagggca atctccgcaa ctggttcacg cctgaggatt tgaaggcgtt
     1621 ccaggcgcgc acagactgcg tcgccaccga atacagcgag ttcaatgtgg cgcccggaat
     1681 caacatcaac ggcaagctcg tcaacggcga atccgtcgcc gacctcggcg gcgccacgat
     1741 cgcgttgcgc gcatacgaga agtcgctcga aggcaaagag cgcaagacga tcgacggctt
     1801 ctcacccgag cagcgcttct tcctcggctt tgcccaagtc tggggcgaga acatggccgc
     1861 gcaggaagcg acccgccgcg cgctgaccga cccgcacgcg caaggcccgt tccgcgtcaa
     1921 tggcacggtc cagaacatgc cggagttctg gaaggcctat cactgcaacg agggcgacaa
     1981 gatggtgcgc gaggcatcga agcgctgttc gatctggtag atttttttga tccgcagatg
     2041 acgcagatgg gacgcagata aaagctgcgt cctaatctgc gttcatctgc gacatctgcg
     2101 gatccgtctc cgaaaccgcc gcgcgaacat cttccatgca catctccatc cgctcgcggt
     2161 gggtgcggtg ggagacgacg cagatgcgga tgacgaagcg gccggcgacg gttgcgccgg
     2221 tcagcatgac gcgttgccgg gcgttgatgc gggcgatcag atcgtggttg agcgcgtcga
     2281 ttgcagcgtc atcgaggccg gggtgtacca ggcgaaatgc cgtgatgctg agctgcggct
     2341 cggcgatgac ttcgatgccg ttgatcgtgt gcaactgcgc ggcggcccat tcggtgagat
     2401 cgagtttctc ctcgagttgt tggcggaagg gctcgatgcc gaacagcttc agtggcagcc
     2461 agacgcggag tccgcggaag tcgcgcgaca gctcggggga gagctcgcag aagtcgatca
     2521 gctc
//
LOCUS       DATLVW010000741         4437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_157276, whole genome shotgun sequence.
ACCESSION   DATLVW010000741 DATLVW010000000
VERSION     DATLVW010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157276"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VNN25_21635"
     CDS             <1..499
                     /locus_tag="VNN25_21635"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXH94193.1"
                     /translation="DEFALQAFDVHAVDYLLKPFSRERFDVAFSRARTRLEDGEDEAG
                     KLATLLASLPQTASHLERIVVRHGDRLFFVTTKDVVHLTAEGNYVRLHTAEGSHLVRG
                     TLADLETRLDPKRFARIHRSGIVNIEEIKEVQSHFHGDYIIVLRSGETLRLSRRYQER
                     LLGEK"
     gene            542..2068
                     /locus_tag="VNN25_21640"
     CDS             542..2068
                     /locus_tag="VNN25_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXH94194.1"
                     /translation="MNTDDDARDLANLGYTQQLFREMGGFSNFAVSFSIISILTGAVL
                     LFGYGLKFAGPIANSVGWPVVSLLTMTVAMSMAEIASAYPTAGGLYFWAFRLGGRTWA
                     WVTAWLNMIGQVSITAGINVAAAIYIIGIFTREPKSWWFQVGVMVLILIPQVAINLWS
                     IRLTARLSDISVWWHIGGVLLIALALTIFGKHHNPASFLFAWKPVVHGAEIAAAPLTI
                     GDFTIRSPLFRLFPSLAMLYATTPPLLFFALAMLQAQWTFTGYDASAHLAEETVMARK
                     NTAWGIVMSVGVSAIAGYVMLMILTWCIPSGDIATTANDPYPVLYIVSKNLAPFLANI
                     IAVVIGVAMWLCGVASVTSMGRMWYAFARDDGMPGSRWLKRVNGTHRTPAVAIIVTSV
                     LSVITCLYAAAWFVVTSISTICLYLAYGIPVLLNLRNRLRGTGEHMTPSVAAWSLGTW
                     SSPINVIAVIWIVIITIVFMLPPNELVLWTMIGVSIVLIAVWMLLARRNFRGPARATQ
                     "
     gene            2272..3015
                     /locus_tag="VNN25_21645"
     CDS             2272..3015
                     /locus_tag="VNN25_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94195.1"
                     /translation="MAIASDRAEVTALDVRYGTAVASAAAKGTSVITADLEDETAKKS
                     RVVISRDSSEIYRLASGDNQLYATFHSLVEAGIRIPTDSKWDAIRPAIDALLFAYYQK
                     EVRFGALTLDESGLAHFGDCHLILKTDMIDFRTTAFTENTAAFVVRNSFVIPPGHRAS
                     WAQRGRLAVAKLASMLSPSTTSADLPAVLKSDGATSGDDAFVEAHVYGSISMRTVEQV
                     SISITATPAVRLRALSEKLKKLGVPLVVA"
     gene            3097..>4437
                     /locus_tag="VNN25_21650"
     CDS             3097..>4437
                     /locus_tag="VNN25_21650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH94196.1"
                     /translation="MDRDHTAVPGSFSDLPSVFGDARSIDFISVASADEALETLRRHH
                     IRQECLDLALILLDRDLSDETRTETAATLNHMLLDSDLRSWLEGVLYGAPLPSEADMD
                     GALRCAQGVGGSTAAAMLYRLSDLQPTIERVSEAWNSVCARVLRLPDSGHEATVLAAR
                     NGVFRHLVHAAEGARYDEFRFAALSVLQPVTNYRRIVEELIAELKLLPASRKVTLRVT
                     AEPESLRWVPLDGPREGERLSIDRMSVFANVQQKKEAIVDRMRAADFDLVERLVDELI
                     EYQLPLDRKAENTSKSLCDLAMEAKALELFELQLELANRAVGVRHQDGWAWAQVGDAS
                     LILNKFDDALTAYQLAGEFGNNLIARKGHASVLLSMGMLEESLAAFEDTIRDYPYDEV
                     AKGGRAEVLKSMGRFDEALAAYDSLIQEFPSNVVAKNGRAEVLKSMGRPDEALAA"
BASE COUNT          851 a         1398 c         1279 g          909 t
ORIGIN      
        1 cgacgagttc gcgctgcagg ccttcgacgt tcacgccgtc gattacctgc tcaaaccgtt
       61 ctcacgcgag cgattcgacg ttgccttcag ccgtgcgcgg acgcggctcg aagacggtga
      121 agacgaagca ggcaagctcg cgacgttgct ggcttcactg ccccagaccg cctcacacct
      181 ggagcgcatc gtcgtccgcc acggcgaccg gctcttcttc gtgaccacga aggacgtcgt
      241 gcacctgacc gccgagggca attacgttcg cttgcacaca gccgagggct cgcatcttgt
      301 gcgcggaaca ctggcggatc tcgaaacgcg tctagatccg aaacgcttcg cccgcattca
      361 ccgctccggc atcgtcaaca tcgaggagat caaggaagtg cagtcgcact ttcacggcga
      421 ctacatcatc gtgctgcgca gcggcgagac cctgcgcctg tcgcgtcgat accaggaacg
      481 cctcctcggc gaaaaatgac ctctgtgctc tctgtgcctc tgtggttaag cttctaacag
      541 aatgaatacc gacgacgacg cccgcgatct tgccaacctc ggctacacgc agcagctctt
      601 ccgcgagatg ggcggcttct ccaacttcgc cgtctccttc tcgatcatct cgattctcac
      661 cggcgcggtg ctgctgttcg gttatgggct caaattcgcc ggcccgatcg cgaactccgt
      721 cggctggccg gtcgtcagcc tgctcacgat gaccgtggcg atgtcgatgg cggagatcgc
      781 ctcggcctat ccgaccgccg gcggtctcta cttctgggcc tttcgcctcg gcggccgcac
      841 ctgggcctgg gtcacagcgt ggctgaacat gatcgggcag gtctcgatca cggccgggat
      901 caacgtcgcc gccgccatct acatcatcgg catcttcacg cgcgaaccga agagctggtg
      961 gtttcaggtc ggcgtcatgg tgctgatcct catcccgcaa gtggccatca acctctggag
     1021 catccgcctc accgcgcgtc tctccgacat cagcgtctgg tggcacatcg gcggcgtcct
     1081 tctcatcgcg ctggcgctga cgatcttcgg caagcaccac aacccagcgt cgtttctctt
     1141 cgcgtggaag ccggtggtgc acggcgccga gatcgcagca gcgccgctga cgatcggcga
     1201 cttcaccatt cgatcgccgc tcttccgtct cttcccttcg ctggcaatgc tatacgccac
     1261 cacgccaccg ctcctcttct tcgcgctggc aatgctgcaa gcgcaatgga ccttcaccgg
     1321 ctacgacgcc tcggcgcacc tcgcagaaga gacggtgatg gcgcggaaga acaccgcgtg
     1381 gggaatcgtg atgtcggtcg gcgtctcggc catcgccggc tacgtcatgt tgatgatcct
     1441 gacgtggtgc atcccctcgg gggacatagc aacaacggcg aacgatccct atcccgttct
     1501 ctacatcgtc tcgaagaacc tcgccccctt cctcgccaac atcatcgccg tggtcatcgg
     1561 cgtggccatg tggctctgcg gcgtcgcctc ggtcacctcg atgggaagga tgtggtacgc
     1621 cttcgcccgc gacgacggca tgcccggctc gcgctggctc aaacgcgtga acggaacgca
     1681 tcgcacgccg gcagtcgcca tcatcgtgac gtccgtgctc agcgtcatca cctgtctgta
     1741 cgccgccgca tggttcgtcg tgacgtcgat cagcaccatc tgcctctacc tcgcctacgg
     1801 catccccgtc ctgctcaacc tgcgcaaccg cctccgcggc accggcgagc acatgacccc
     1861 ctccgtcgcc gcctggtctc tcggcacctg gtcctcgccc atcaacgtca tcgccgtaat
     1921 ctggatcgtc atcatcacga tcgtcttcat gctcccccca aacgaactgg tcctctggac
     1981 gatgatcggc gtctcgatcg tgctgatcgc ggtctggatg ttgctcgcgc gacggaactt
     2041 ccgcggtccg gcgagggcga cgcagtaagc cgggaagcgc ggtccacgag gctcaccggt
     2101 gctgccggta gcgctgttcc gcagttatgc ctagaatggc tgatcaaccc aactatttga
     2161 ggtgaaatgt tgagctctgg caggactcaa acgtgcacgt tttgtagttt cagcatgccg
     2221 atctctgcca atcgatgccc tcactgttcg cgaccgtccc tttttccaaa cgtggcgatc
     2281 gcatctgatc gagccgaggt gactgcgctt gatgttcgct acggcactgc cgtcgcgagt
     2341 gcggcggcaa aggggacaag tgtcatcact gcagaccttg aagatgagac agccaagaag
     2401 tcgcgagtgg tcatttcgcg ggactccagc gaaatctacc gtctcgcctc aggtgacaac
     2461 cagttgtacg ctacattcca cagcctcgtc gaggctggca tccgcattcc aacggattcg
     2521 aaatgggacg ccattcgtcc cgccatcgac gccttgctct ttgcctacta tcagaaggag
     2581 gttcgcttcg gcgcgctgac tctggatgag agcggactcg cgcacttcgg tgactgtcac
     2641 ttgatcctga agaccgacat gatcgacttt cgaacaacag cattcacgga gaacacagcc
     2701 gcgtttgtgg tgaggaacag cttcgtgatt ccgccaggac atcgtgcgtc atgggcgcag
     2761 cggggcaggt tggcagtcgc caaactggct tcgatgctat cgccgtcgac aacatctgcg
     2821 gatttgcccg ctgtgctgaa atccgacggg gcaacgtccg gcgacgacgc gttcgtggag
     2881 gcgcacgtct acggctcgat ttccatgcga accgttgagc aagttagtat ctcaatcacc
     2941 gcgacacctg ccgtcaggct gagggcgctg agtgagaagc tgaagaaatt gggggttcca
     3001 cttgttgtcg cgtgagaggt tttgatgcag gcagtcctaa acggcatgtc tggaagaggt
     3061 ctcctcatcg agggaggtca agcgtcgctt ctccgcttgg accgcgatca cacagcagtt
     3121 cccggaagtt tcagcgatct gccgtcagta ttcggcgatg cccgcagcat tgattttatc
     3181 agcgtcgcta gtgctgatga ggcactcgaa actctgagac gccaccacat tcgccaggag
     3241 tgccttgatc tcgcgctcat cctgctcgac cgtgacctgt ccgacgagac acgaacagaa
     3301 actgcagcga ccctgaatca catgctgctc gattcggatc tcaggtcatg gctcgaaggc
     3361 gttctctatg gagcacctct cccatccgag gccgacatgg atggagcact tcgatgcgct
     3421 cagggagtag gaggaagcac agccgcagca atgctgtatc gcctgtcgga cctgcagccc
     3481 acgatcgagc gcgtgtctga agcgtggaat tccgtctgcg ctcgcgtctt gcgtttaccg
     3541 gattctggtc atgaggccac cgtcctcgcg gcacgcaatg gtgtcttccg gcacctcgtc
     3601 cacgccgccg aaggcgcccg ctacgacgaa ttcaggttcg ccgcgctaag tgtgctgcag
     3661 ccagtaacca actaccgacg aatcgtcgag gagttgatcg ccgagttgaa acttcttcca
     3721 gctagccgga aggtgaccct gcgcgtgaca gcggagcctg aatctctgcg gtgggtgcca
     3781 ttggacggtc ccagagaggg tgagcgtctc agcatcgacc gcatgtcggt attcgcaaat
     3841 gtgcaacaaa agaaagaggc gatcgtggac cgcatgcgag cggcagactt cgatctcgtc
     3901 gaacgacttg tcgatgaact catcgaatac caacttccac tcgaccgcaa ggcggaaaac
     3961 acgagcaaat cgctgtgcga tttggcgatg gaagctaagg ccctggaact ctttgaactg
     4021 cagttggaac tggccaaccg tgctgttggc gttcgtcacc aggatgggtg ggcgtgggca
     4081 caagtcggtg acgcctcatt gatcctgaac aaatttgacg atgcgctgac cgcctatcaa
     4141 ctcgccgggg agttcggtaa caatctgatc gcccgaaaag gccacgcgag cgtacttcta
     4201 agtatgggaa tgctggagga atcgcttgcc gctttcgagg acacgattcg tgactacccg
     4261 tacgacgagg tggcgaaggg cgggcgcgca gaagtgctca agtctatggg gcggttcgac
     4321 gaggctcttg cggcgtatga ctcactcatc caagagtttc caagcaacgt tgtcgcgaag
     4381 aacggacgcg cggaagtact caagtccatg ggacgcccgg acgaagcgct cgcggcc
//
LOCUS       DATLVW010000742         4730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_442032, whole genome shotgun sequence.
ACCESSION   DATLVW010000742 DATLVW010000000
VERSION     DATLVW010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442032"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VNN25_21655"
     CDS             complement(<1..909)
                     /locus_tag="VNN25_21655"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HXH94197.1"
                     /translation="MSPGTFNLVIDNQTEKYSNNEVYIYVIGHNPNSNNVFAWLNASG
                     TLTDAPPSSSPANLPLNASGTTTFTVPYITSGRIWISLGAALIMPFNSDNPPGQIQPS
                     VSNPSDPNINALWDFAEFNFDSTSLFVNTSQVDAFAIPIQLTLDGTVASTPVSVVGVD
                     GAGWTAVQKAVLANPTFSPLVINNANGDFVRVLQPNDGITYGVFDASYLDVYIDSCWT
                     FYQTNEMIMDLTAAGFGKVVGTVDFNSNFNFLTQDTNSLLKIVATIPKPTTQQAFACN
                     GPLATGNDEQQAIQNVIAAGLNRGVLE"
     gene            1269..3431
                     /locus_tag="VNN25_21660"
     CDS             1269..3431
                     /locus_tag="VNN25_21660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH94198.1"
                     /translation="MSTKTSGRRSEARDRLLAHVAFDAIWERDLQSGVVTWDRSLESI
                     FGYHLDEVVNHVNWWRERVHPDDIEHLEQNVAQAIESGASGWSNEYRFLRKDGSWAWV
                     ASRCAVERDADGRACYAVGAMIDISQLKDTETRLRLFTEQIPGRACVTDRELRVVWDA
                     GVAFPGSPSAVGKTVPELFAQSPDRDRVLEGCRQALAGQSSKMEIDDGTSAAQLELEP
                     FRDPAGNVVGIAGIALDITDRVRSEEQVRAGQRLLRRVLETLPVGVVVLNRAGDVVLH
                     NPASTQIWGVTIASGEERWARSKGFRHGTAQAVTPGEWASHRALDEGQTTRDELIDIE
                     TFGGERKTIENYAAPILDADGVITGAVVINEDVTERVRAEEALRNTERLLIDAEKLGQ
                     TGSWEQDLISGQVFNTEANGRLFFGDDRSKGTRMEDYVEAVHPDDRGWVMRRRELLDG
                     GTGPDTIEYRVIWPDGSVHWIFGRATVVRDAAGRPVRAYGTNADVTERKNAEEELGRR
                     AKQLETLSRKLIEAQEAERRVVARELHDDFGQVLFALKLNLQRLQCDSSESVELVDGA
                     IARMRNLAQALRPPLLDELGLEEALRWHVEHEAKRAGLAFRIAIAPLGRRLPAVVEMT
                     CFRVVQEALSNVIRHAQAHLVEVELSEANGSLELVVRDDGRGFDVSAARKRAIEGESQ
                     GLLSMQERATLAGGDLRIDSVPGGGTCVHGRLPLGEES"
     gene            4295..>4730
                     /locus_tag="VNN25_21665"
     CDS             4295..>4730
                     /locus_tag="VNN25_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94199.1"
                     /translation="MKTISAPILLFYAATMASAQFTGPLATGVRLDPAGEAIDLGSMP
                     LGMTLAPGGGKLVVVLSGWREQGLQIVDLKTRTVSQTIKQEAAFYGIAFAPDGGHLYV
                     SGGNDDSIFCYTWKDGVAAPERKIVLGKQKEDKTGSRYPAGLA"
BASE COUNT          926 a         1399 c         1568 g          837 t
ORIGIN      
        1 ctccaggact ccgcggttca ggcccgccgc gatcacgttc tgaatggcct gctgttcgtc
       61 attgcccgtc gcaagcggtc cgttgcaggc gaacgcctgc tgcgtcgtcg gcttcggaat
      121 cgtcgcgacg atcttcaaga ggctgttggt gtcctgcgtc agaaagttga agttgctgtt
      181 gaagtcgacc gtccccacca ccttgccgaa tcctgccgcg gtgagatcca tgatcatttc
      241 gttggtctgg tagaaggtcc agcaactgtc gatgtagaca tcgagatagc tggcatcgaa
      301 aacgccatag gtgatgccgt cgttcggctg caggacgcgg acgaaatcgc cattcgcgtt
      361 gttgatgacg agcgggctga aggtggggtt tgcaaggacg gccttctgaa cggccgtcca
      421 gccggcgccg tcgacgccga ccaccgacac tggcgtcgaa gccacagtgc cgtccagcgt
      481 caactggatc ggaatggcga acgcgtcgac ctgcgatgta ttgacgaaca ggctcgtgct
      541 gtcgaaattg aattccgcga aatcccatag cgcgttgatg ttcggatcgc tcgggttcga
      601 aaccgacggc tggatctggc ccggtgggtt gtcgctgttg aacggcatga tcagcgccgc
      661 gccgagggag atccagatcc gaccgctggt gatgtagggg acggtgaacg tcgtggtccc
      721 ggacgcgttg agaggaaggt tcgccggcga actactcggc ggcgcatcgg tgagcgttcc
      781 gctggcattc agccaagcga atacgttatt gctgttcgga ttatgcccga tgacgtaaat
      841 atagacctcg ttattcgagt acttctcggt ctggttgtcg atgacgagat tgaacgttcc
      901 agggctcatg ctgtctctcc tcatcagagg agagccggta ttgcgttcat tccctaccca
      961 tgcggcccaa acggccgccg atcgccggca cgggaggcgg ttgagattgc tgacgcagtc
     1021 gcccgatcct tgaacgccgg ggaggcagga tcacgagcat gcgcttcttc tcagagggac
     1081 tgctccccag ccgcgagagg acctgcatga tcgccgttga tacgaacgtc ctcatccacg
     1141 ctgggatcat tctgatccga ggtgtcaggt cgaaactaaa accgaagacc cttccgggcg
     1201 ccgcgcgatc gtcctcgatc acgatccggg cgataatgaa acacccggaa acactgtacc
     1261 gcctgcatat gtccacgaag acttcaggtc gacgcagcga agcgcgagac cggttgctcg
     1321 ctcacgtcgc cttcgatgcc atctgggaga gagatctcca aagcggcgtg gtgacctggg
     1381 accggagtct ggagtcgatt ttcggctacc acctggacga ggtcgtgaac cacgtcaact
     1441 ggtggcgcga acgcgtccat cctgacgaca tcgagcacct cgaacaaaac gtcgctcagg
     1501 caatcgagag cggcgcctcc ggctggtcga acgaataccg attcctccgc aaggacggct
     1561 cctgggcgtg ggtggcctcg aggtgcgcgg tcgaacgcga cgccgatggg cgcgcctgct
     1621 acgccgtcgg cgccatgatc gacatcagcc agttgaagga taccgagacg cgcttgcggc
     1681 ttttcacgga acagattccc ggccgggcct gcgtgacgga tcgcgagctg cgcgtcgtgt
     1741 gggacgcggg ggtagcgttc ccgggcagcc cttccgcggt gggaaaaacc gtccccgaac
     1801 tattcgcgca atcccccgac cgcgaccgtg tcctggaggg gtgccggcag gcgctggcgg
     1861 gccagtccag caagatggag atcgatgatg ggacgagcgc cgcgcagctc gagctcgagc
     1921 cgttccgcga tccggccggg aacgtggtcg gcatagccgg catcgcactc gacatcaccg
     1981 accgcgtgcg cagcgaggag caggtccgag ccggccagcg gctcctgcga cgcgttctcg
     2041 aaaccctccc ggtgggtgtc gtcgtgctga atcgagcggg agacgtcgtg ctgcacaatc
     2101 ccgcctccac tcaaatctgg ggcgtaacga tcgcgtcggg ggaggagcgg tgggccagga
     2161 gcaaggggtt ccgccacggc acggcgcagg cggttacccc gggagagtgg gcttcgcacc
     2221 gcgcgctcga cgaggggcaa accacccgcg acgaactgat cgacatcgag acgttcggcg
     2281 gggagcgcaa gaccatcgag aattacgcgg cacccattct cgacgcggat ggcgtcatca
     2341 ccggtgcagt cgtgatcaac gaggatgtga cggagcgcgt gcgtgccgag gaggcgttgc
     2401 gcaacaccga gaggctgctg atcgatgcgg agaagctggg gcagaccgga agctgggagc
     2461 aggacctgat cagcggacag gtcttcaaca cggaagcgaa tgggcgcctc ttcttcggcg
     2521 acgaccggag caaaggcacg cggatggagg attacgtcga ggccgttcat cccgacgacc
     2581 gcggctgggt catgcgccgc cgcgaactgt tggatggcgg tacggggccg gacaccatcg
     2641 agtaccgcgt catctggccg gacggcagtg tgcactggat cttcggccgc gccacggtcg
     2701 tccgcgacgc ggcaggccgg ccggtccgcg cctacggaac caatgccgac gtcaccgagc
     2761 gcaagaacgc cgaggaggag ctggggcgga gagcgaagca actggagacg ctgtctcgca
     2821 agctgatcga agcgcaggag gcggagcgcc gcgtggttgc gcgcgagctc cacgacgact
     2881 tcgggcaggt gctgttcgcc ctcaagctca accttcaacg actgcagtgc gatagctccg
     2941 agagcgttga actggtcgac ggcgccatcg cgcgcatgcg taatctggcc caggccctcc
     3001 gaccgccgct gctcgacgag ctcgggctcg aggaggcgtt gcggtggcat gtggagcacg
     3061 aggcgaaacg cgccggactc gcgttccgca tcgccatcgc tcccctcggg cggaggctgc
     3121 cggccgtcgt ggaaatgact tgcttccgcg tcgtgcagga ggcgctgagc aatgtcatcc
     3181 gtcacgcgca ggcgcacctc gtcgaggtcg agctgagcga ggccaacggg agcttggaac
     3241 tcgtggtgcg cgatgacggc cgcggattcg atgtgtctgc cgcgcgcaag cgcgccattg
     3301 agggagaaag ccagggcttg ctcagtatgc aggagcgcgc cacgctcgct ggaggagatc
     3361 tgcggatcga ctcggtaccc ggcggcggca cgtgcgtaca cggtcgcttg ccgctcgggg
     3421 aagagagttg agtgtgagct tggtgtttgt acccattcgg tcaatttgaa aaaccggtca
     3481 ccacagaggc acagaggtca cacagacccc tctgtgttct ctgtgtctct gtggttgcgt
     3541 agtgactgaa agtcggcaca tcgctcgcac ctcttattga cgttgcccaa gctcgtgccc
     3601 gaagcgacgt caacggaagg aggacacaat gcgcaacaga attcttacgg tcgttgccgc
     3661 gggcatgctc gtctgtatgc ctttggcagc acagcgaaat aagaacgccg gcggtatggg
     3721 tgctatgcct gcgatgccga tgaacacacc gagagcagtg agcacgaatt cagtgcagag
     3781 tccttcgcca ggcacaacgg tgaggacgca tacagtaacg aaccctgctg gatcggtcac
     3841 caggaccgcg accaggtccg gcagcggctg gactcggacg atggtgaaca cagaaggaaa
     3901 caagacgctc acgatgtccc acagcaaggt gggacgatcg cataccagga cgcggtcgct
     3961 cgtccacagt cagaaggcgg cgaaagtgaa ccgcggtcat cgagggaagt ccggaaggta
     4021 gagagcgcag tggaaggtag tgcgacggtc aggtcttcac tcgatgaaga ttcggtggag
     4081 acctgacttc acccagattt cgacttcgtg cacgccgcca tcaggccgat cgccagcgta
     4141 ccgagcgccg cgttccgccc gagaggggtc aggtcgaaac cgaaaccgac gactcctcgc
     4201 tacgaacatt gcgttcgatg caaactgtcg tcagtttcgg tttcgacctg acccctctcg
     4261 gatactcttc gcagatactg ctgaggaggc caatatgaag acaatctccg ctccgatcct
     4321 cctgttttat gcggcaacga tggcgtccgc acaattcacc ggtccgctgg ctaccggcgt
     4381 ccgtctcgat ccggctgggg aggcaatcga ccttggcagc atgccgctgg ggatgacgct
     4441 tgcgccgggc ggcgggaagc ttgtcgtcgt gctgagcggc tggcgcgagc agggtttgca
     4501 gatcgtcgat ctgaagactc gtaccgtctc gcagacgatc aagcaggagg cggcgtttta
     4561 cggcatcgcc ttcgcgccgg acggcgggca tttgtacgtc tccggaggca acgacgattc
     4621 gatcttctgc tacacatgga aagacggcgt ggccgcgccg gagcggaaga tcgtcctcgg
     4681 caagcagaaa gaggacaaga ccggctctcg atatccggcc ggtctggccg
//
LOCUS       DATLVW010000743         6338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_484509, whole genome shotgun sequence.
ACCESSION   DATLVW010000743 DATLVW010000000
VERSION     DATLVW010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484509"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="VNN25_21670"
     CDS             complement(<1..1823)
                     /locus_tag="VNN25_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH94200.1"
                     /translation="MNPSRIFILRPVATSLLMAAILLAGFVAFRQLPVSALPQVDYPT
                     IQVLTFYPGASPDVMTSSVTAPMERQFGQIPGLTQMSSTSSFGGSLITLQFDLKQNID
                     VAEQEVQAAINAAASFLPRDLPNPPIYSKVNPADTPILTLALTSTQLPLSKVEDLADT
                     TLAQKISQLPGVGLVTISGGQKPAVRVQANPSALASYGLSLEDLRTGLAAANTDQAKG
                     NLQGPSQSFSIGANDQIYASADYKPVIIAYRNGAPIRVGDVASVVDGVENAQQAAWMN
                     ATPAVIMNVQRQPGANIINVVDRIEQLLPQLSASLPPTVKVTVLTDRTVTIRASVHDV
                     ELELLLTIALVVMVIFLFLRRLSATIIPSVAVPLSIVGTFGVMYLLGYSLDNLSLMAL
                     TISTGFVVDDAIVMIENITRYIEEGHAPLEAALMGSKQIGFTIVSLTVSLIAVLIPLL
                     FMGDIVGRLFREFAVTLSVTILVSAVVSLTLTPMMSAKLLKPDKKGEESRFYLKSEEL
                     FNRVIAYYGRTLQFVLRHQTTTLIVTFATLVLTLILYIVVPKGFFPVQDTGVILGISE
                     APETTSFARMADRQQALADVVTKEPDVESVSSFIGVDGTNMT"
     gene            complement(1820..3136)
                     /locus_tag="VNN25_21675"
     CDS             complement(1820..3136)
                     /locus_tag="VNN25_21675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH94201.1"
                     /translation="MADREVSYEVPNPAARQAPKPVEDDDRNATARPNRRGRFILIAI
                     GIVIAIVIVVALRKRSATEAQTSKEAAAAGKNRASTVAVAPVEQRDVPVYLDGLGNVN
                     ALNTVTVKTRIDGQLERFNFQEGQMVHAGEELALIDPRPSEAALAQAQATKFRDEATL
                     ENTRRDLQRFADLYKAGVIPQQQYNTQQSQLRVNEGSVKADAAQVQNAQLNVSYCHIK
                     SPIDGRIGIRSVDPGNMVHASDPNGLIVITQLQPIAVLFTVPEDSLPAVAQHMRGGLP
                     VEAWSRDSTQKLADGRLLTIDNQIDPNTGTFRCKAVFDNKDGALFPNQFVNARLRVDV
                     RHNALTVPAAAVQHGSQGTYVYVVRPDKTVDARPVAVVMTEGTTSVLGGGVNPGDQVV
                     TDGADKLQPHGKVEIAGAGGGRGGGRNGGAGKGAPGAGRRKQAGQS"
     gene            complement(3357..4718)
                     /locus_tag="VNN25_21680"
     CDS             complement(3357..4718)
                     /locus_tag="VNN25_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH94202.1"
                     /translation="MTVTTETTEAMTADEIVALTKRHTIFSWSAQGSVNPIPMVRGEG
                     IYFWDANGKRYMDMNSQLMCVNIGHGDRRVIDAIKRQAEELAYAGPGMATPVRARVGK
                     MLADLTPGDLNRFFFTLGGAEANENAIRVARMVTGRQKIMARYRSYHGATSGAISLTG
                     DPRRWANEPGLPGVVRFFDPYKYRSHLFREGDSDEMFTRRCLDEIEETLMYEGPQTIA
                     AIFVETVTGTNGLIVPPDGYLQGLRQICDRHGILLVCDEVMCGLGRTGKWFAADHWNV
                     VPDMITMAKGLTSAYLPLGALAMSDRIANYFDKNTFFGGLTYNAHPMSLAAAEACLQA
                     MIDDDTMVHTQKMGRVLRELHGVMKDRHPSVGDVRSIGLFGVLELVRNRITKEPMAPF
                     NGTSPEMQKLGAFLKENGMYAFINWNNLFTNPPLCITEAQLREAFELIDRGLEITDAA
                     VTG"
     gene            complement(4921..5322)
                     /locus_tag="VNN25_21685"
     CDS             complement(4921..5322)
                     /locus_tag="VNN25_21685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94203.1"
                     /translation="MSISLERLAPRVLIVDDDDGIQTLMKALLRRREVIVDSVSDGDA
                     ALRKMRERSYDSIVLDLMLPKVNGFEVVRELKTSSPDLLKRTIVVTAVSNHALANFED
                     SGLVRCVLRKPFDINQFINEVLACATDNGGN"
     gene            5624..>6338
                     /locus_tag="VNN25_21690"
     CDS             5624..>6338
                     /locus_tag="VNN25_21690"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH94204.1"
                     /translation="MPSLSRSLLLDTLPPEVRSSLRVQESEHRLSEVLIKADENPEFV
                     FFPHAGSLVSIIRSTESGSMVEVGVIGGEGVFNVHSLLTQAPPTGSEALVQGEGRFSQ
                     VKASRINDLFRTNEPFRELLLEYTSIFLDQVTQNAVCNRLHPIEQRLAKWLLIVRERI
                     GNDDLQLTHDFLAHMLGVHRPGVSIAVNALAQDAVIESRRNHIVVRDVVGLKAKSCEC
                     HAAINGRLSVFSQKLGKRSV"
BASE COUNT         1124 a         1853 c         2081 g         1280 t
ORIGIN      
        1 gtcatgttcg taccgtcgac gccaatgaac gacgagacgc tttccacgtc cggctccttg
       61 gtgaccacat ccgcgagcgc ttgctgccgg tcggccatgc gcgcgaacga cgtcgtctcc
      121 ggtgcctcgg agatgccgag aatcacgccg gtgtcctgga caggaaagaa gcccttcggc
      181 acgacgatgt agaggatgag cgtcagcacg agcgtggcga aggtgacgat cagcgtcgtg
      241 gtctggtgcc gcagtacgaa ctgaagcgtg cggccgtagt aggcgatcac gcggttgaag
      301 agctcttccg atttgaggta gaagcggctc tcctcgccct tcttgtccgg cttgagaagc
      361 ttggctgaca tcatcggtgt cagcgtgagc gagacaaccg cggagacgag aatggtgacg
      421 ctgagcgtga ctgcgaactc gcggaaaaga cgcccgacga tgtcgcccat gaagagcaga
      481 ggaatcagca cggcgatcag cgagacggtc aatgacacga tggtgaagcc gatctgcttc
      541 gagcccatca acgcggcctc gagcggtgcg tggccctctt cgatgtagcg cgtgatgttc
      601 tcgatcatga cgatggcgtc gtcgacgacg aagccggtgg agatggtcag cgccatgagg
      661 gagaggttgt cgagggagta gccgaggagg tacatcacgc cgaaggtgcc gacgatcgac
      721 agcggaacgg cgacgctcgg aatgatggtt gccgacaggc ggcgcagaaa caggaagatc
      781 accatcacga cgagagcgat cgtgagcagc aactccagct ccacgtcgtg taccgaggcg
      841 cggatggtga cagtgcggtc ggtgaggacc gtgaccttga ccgtcggcgg aagacttgcg
      901 gagagctgcg gcaagagctg ttcgatgcgg tcgacgacgt tgatgatgtt cgcgccgggc
      961 tgccgctgca cgttcatgat cacggccggc gtcgcgttca tccacgctgc ctgctgcgcg
     1021 ttctcgacgc cgtcgacgac gctggccaca tcgcccacgc gaatgggcgc gccgttccga
     1081 taggcgatga tgaccggctt gtagtcggcg ctggcgtaga tctggtcgtt cgcgccgatg
     1141 ctgaaactct gcgacggacc ttgcaggttg cctttggcct gatccgtgtt cgcggcagcc
     1201 agtcccgtgc gcagatcctc gagactcaaa ccataggaag cgagcgccga cggattggcc
     1261 tgtacgcgca cggccggctt ctgaccgccg ctgatcgtga ccagaccaac gccgggaagc
     1321 tgcgagatct tctgcgcgag cgtcgtatcg gcgaggtcct ccactttgct cagcggaagc
     1381 tgcgtcgatg tgagcgcaag cgtgaggatg ggggtgtcgg cggggttgac cttgctgtag
     1441 atgggcggat tgggcaggtc gcgcggaaga aagctcgccg cggcgttgat cgccgcctgc
     1501 acttcctgct ccgcgacgtc gatgttctgt ttgagatcga actgcagcgt gatgagcgag
     1561 ccgccgaatg agctggtcga gctcatctgc gtaagaccgg ggatctgccc gaattgacgt
     1621 tccatcggcg ccgtgacgga cgaagtcatg acgtcgggac tcgcgcccgg atagaacgtc
     1681 agcacctgaa tcgtcggata gtcgacttgc ggcaacgccg aaacggggag ttggcgaaag
     1741 gcaacgaagc cggccagtaa gatggccgcc atcagcagcg aggtggcgac cggccgcaga
     1801 atgaaaatgc gggatgggtt cacgactggc ccgcctgttt cctcctgcct gctcccggcg
     1861 cccctttgcc cgcgccgccg ttcctgcctc cgccacggcc accgcccgct ccggcgatct
     1921 cgactttgcc gtgcggctgg agcttgtccg ctccgtcggt caccacctga tcgccgggat
     1981 tcactccacc acccagtacc gatgtcgtcc cctcggtcat cacaacagcg accggccgcg
     2041 cgtccactgt tttgtccggc cgcacgacgt agacgtacgt tccctgcgag ccgtgctgta
     2101 ccgccgcagc gggaacggtc agcgcgttgt gccggacgtc gactcgaagg cgggcgttga
     2161 cgaactggtt cggaaagagt gcgccgtctt tgttgtcgaa gacggctttg caccggaacg
     2221 tgccggtgtt cggatcgatc tggttgtcga tggtcaggag acggccgtcg gctagcttct
     2281 gcgtgctgtc ccggctccag gcctccacgg gaagtccgcc gcgcatgtgt tgcgcaaccg
     2341 ccggaaggct gtcctcgggt acggtgaaga ggaccgcgat cggctgcaat tgcgtgatga
     2401 cgatcagccc gttcggatcg gaggcgtgga ccatgttgcc gggatcgact gaacgaatgc
     2461 cgatgcgccc atcgatcggc gacttgatgt gacagtagct gacgttgagc tgcgcgttct
     2521 ggacctgcgc cgcgtccgcc ttgactgagc cttcgttgac gcgcagttgc gactgttgcg
     2581 tgttgtattg ctgttgcggg atcacgccgg ctttgtacag atcggcgaag cgctgcagat
     2641 cgcgccgcgt gttttccagc gtcgcctcat ccctgaattt cgtggcctgg gcttgcgcga
     2701 gcgccgcttc cgagggccgc gggtcgatca gcgccaactc ctcgccggcg tggaccatct
     2761 gcccttcctg aaagttgaaa cgctcgagct gtccatcgat gcgcgtcttg acggtcactg
     2821 tgttgagggc attgacgttg ccgagcccgt cgagatagac gggaacatcg cgctgctcga
     2881 ctggagcgac ggcgaccgtc gaggcgcggt tctttccggc cgctgctgcc tctttcgacg
     2941 tctgcgcttc tgtggccgat cgcttgcgca gcgcgacgac gatcacgatg gcgatcacga
     3001 ttccgatcgc gatcaggatg aatcgcccgc gccggttcgg ccgggcggtc gcgttcctgt
     3061 cgtcgtcttc cacaggcttc ggagcctgtc tcgccgcggg gtttggtact tcgtacgaca
     3121 cttctcgatc agccacggtt tcgtccactc tgcggttcat cgatactcca gcaggccccg
     3181 gcccggacgc attcggtaaa gcaatctctt taccatccgc gtcgcaaatc gttgaacgga
     3241 cgttgctaat cggcgaaagt catcagccag ccgagcgggg cttggggttg tgggctggga
     3301 ttcgcgcgtc ggccctgact cttaaccaca gagacacaga ggtcacagag aaaacgctaa
     3361 cccgtcactg cggcatccgt aatctccaga cccctatcga tcagctcgaa tgcttcgcgg
     3421 agctgtgctt ccgtaatgca cagcggcggg ttggtgaaga ggttgttcca gttgatgaag
     3481 gcgtacatgc cgttctcttt caggaacgcg ccgagcttct gcatctcggg gctggtgccg
     3541 ttgaacgggg ccatcggctc tttcgttatg cggttgcgca ccagttccag gacgccgaaa
     3601 aggccgatgg agcggacgtc gccgaccgag gggtgcctgt ccttcatcac gccgtgaagt
     3661 tcgcggagca cgcgtcccat cttctgcgta tgcaccatcg tgtcgtcgtc gatcatcgcc
     3721 tgcaggcacg cttcggcggc ggcgaggctc atcggatggg cgttgtaggt gagaccgccg
     3781 aagaaggtgt tcttgtcgaa gtagttggcg atgcggtcgc tcatcgccag cgcgccgagg
     3841 gggagatacg ctgacgtcag gcccttggcc atcgtgatca tgtcggggac gacgttccaa
     3901 tgatcggcgg cgaaccactt tccggttctg ccgaggccgc acatcacttc atcgcacacg
     3961 aggaggatgc cgtggcggtc gcagatctgg cgcagaccct ggaggtagcc gtccggcgga
     4021 acgatgagac cgttcgtgcc ggtgacggtc tcgacgaaga tcgcggcgat ggtttgcgga
     4081 ccttcgtaca tcaacgtctc ttcgatctcg tcgaggcagc ggcgcgtgaa catctcgtcg
     4141 ctgtcgcctt ctcggaagag atggctgcgg tatttgtagg gatcgaagaa gcggacgacg
     4201 ccggggaggc cgggctcgtt ggcccagcgg cgcggatcgc cggtgagcga gatcgcgccg
     4261 gacgttgcgc cgtgatacga acggtagcgg gccatgattt tctgccggcc ggtgaccatg
     4321 cgcgccacac ggatggcgtt ttcgttggct tcggcgccgc cgagggtgaa gaagaagcga
     4381 ttgaggtcgc cgggggtgag gtcggcgagc atcttgccga cgcgggcgcg caccggtgtc
     4441 gccattcccg gccccgcata cgccaactcc tcggcctgac gcttgatggc gtcgatgacg
     4501 cggcggtcac cgtggccgat gttgacgcac atgagctgcg agttcatgtc catgtagcgc
     4561 ttgccgttgg cgtcccagaa gtagatccct tcgccgcgga ccatggggat gggattgacg
     4621 ctgccctggg cggaccagga aaagatggtg tggcgcttgg tcagggcgac gatctcatcg
     4681 gcggtcatcg cttcggtggt ttctgtggtt accgtcatct cgatgctcct cgggcgaagt
     4741 aatagccggg tgggagttga aatgcaatgt tttgcatcac gggggagggg gcagagacaa
     4801 gagacaagag atcagagaca aggggccgag gcgaagggac actgagaatg gatgagggac
     4861 tcgggagaga acgagagaac ttacccttgt ctcttgtctc tgatcccttg tctctgcccc
     4921 tcagttcccg ccgttgtccg tcgcgcaggc cagcacttcg ttgatgaact ggttgatgtc
     4981 gaacggtttg cgcaggacgc agcgtacgag cccgctgtct tcgaagttgg cgagcgcgtg
     5041 attcgagacg gcggttacga cgatcgttcg cttcagaaga tcgggcgagc tggttttcag
     5101 ttcccggacg acttcgaagc cgttcacctt tggaagcatc aggtcgagaa cgatcgagtc
     5161 gtagctccgt tcgcgcatct tgcgaagggc cgcatcccca tccgagacgc tgtcgacgat
     5221 gacctcgcgc cgtcgcagga gcgccttcat gagcgtctgg ataccgtcat cgtcatcgac
     5281 gattagcact cggggagcga ggcgttccag ggagatcgac atggggcacc gcccggttgt
     5341 ttccgggacc aggaatcggg attgcttgtc gaagcgatca aggacggtac gctcgtcgcc
     5401 cccccccccg tctgttcgat atcgaacaaa atccagccta ccgattaaga gagcattcgg
     5461 gcctcccgct gctgcggaat ctggcgatga gcagggcagt tttgaaccgg tgcagttgca
     5521 cggtcggccc gccgatcctt cggtatttgg cactgacgtc ataagcgaac gtctcttcct
     5581 gatctcgtcg cggttcacgt aacccgtcgc ttccggagga gcagtgccat ctctcagccg
     5641 tagtcttctt ctcgataccc ttccgcccga ggttcgaagt tcgctgcgcg tgcaggaatc
     5701 cgaacatcgt ctgtccgagg ttctgatcaa agcggacgaa aatcccgagt ttgttttctt
     5761 tccgcacgcc ggatcgctcg tgtcgatcat ccgttcgacg gagagcggtt cgatggtgga
     5821 ggttggcgtc atcggtgggg aaggtgtctt caatgtccac tccctcctca cgcaagcgcc
     5881 tcccaccgga agcgaggcgt tggttcaggg cgaagggcgc ttctcgcagg tgaaggcgtc
     5941 ccgcatcaac gatctctttc gcaccaacga gccttttcgc gagttgctgc tcgaatacac
     6001 gagcatcttt ctcgaccagg tgacgcagaa cgcggtctgc aaccgattgc atccgatcga
     6061 acagcgtctg gccaagtggc tgctgatcgt gagagagcgc atcggcaacg atgatctgca
     6121 gctcacgcac gactttctcg cgcacatgct cggcgttcac cgcccgggcg tttccatcgc
     6181 cgtgaatgcg cttgcgcagg acgccgtgat cgagagccgc cggaatcaca tcgtcgttcg
     6241 cgatgtggtg gggctgaagg cgaagtcgtg cgagtgccac gccgcgatca atggccggtt
     6301 gagtgtgttc tcgcagaaac tggggaagag atcggtgg
//
LOCUS       DATLVW010000744         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_114252, whole genome shotgun sequence.
ACCESSION   DATLVW010000744 DATLVW010000000
VERSION     DATLVW010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114252"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1777)
                     /locus_tag="VNN25_21695"
     CDS             complement(107..1777)
                     /locus_tag="VNN25_21695"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXH94205.1"
                     /translation="MKAVISVQRSVIRSAIAAVALFVAACATQPKPLALDLKIINGRI
                     IDGTGAPWYRGDVGVRGDTIVSIGDLSHVNATATIDAHDNIVSPGFIDLLGQSQGSVF
                     QDPHLEAKVRQGVTTEVTGEGHSPGPLNQKQIDVSSPNNRPKWHTLGEWFSVLEKSGT
                     AINFALFVGADNPREMVIGTVNRAPTADEMREMERIVDQAMREGAIGLSTSLIYLPAM
                     FSTTEEIINLAKVAAQYGGVYFSHIRDEGDHIDTALDEAFRIGREAKIPVNIWHLKVG
                     GRANWGRMPHVIERINAARAEGLDVAANVYPYAASSTSLSTLVPDWSLEGGYTELKKR
                     LADPAQRARIAEALKAQYAKRGPKGIYITRIGAPALVQYEKHFVEDIATMMNVPPEEA
                     MMRLFAETNGSPGVIFFSMNEDDVQTALKQSFVSIGSDSGAPPPIARATSAGSHPRAY
                     GTFPRVMGHYVRDEHLFTLEEAVRKATSQAADRTNLTDRGVLRPGMKADVVVFDANNI
                     RDVSTYEDPHHFSEGVLDVIVNGVPVLRDGTMTNALPGHVLRGRGYVK"
     gene            complement(1774..3045)
                     /gene="lysA"
                     /locus_tag="VNN25_21700"
     CDS             complement(1774..3045)
                     /gene="lysA"
                     /locus_tag="VNN25_21700"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXH94206.1"
                     /translation="MLSFIDHTLHIGNVSTIDLAKRFGTPLYIYDAEIVRRQIERVKR
                     AFTALPFRPFYAMKANGALAVLDLMRGAGFGCDTVSPGEIFIAQKAGYTADNIWFTCS
                     NVSDDDLRAIGDERIVINVNSMSEIDRILALDLRNPIALRVNTDIGAGHHHDVVTAGG
                     GVKFGIDLAEIESARMIAEDSGRKVVGLHAHIGSGIDTIAPLIESARRLLDLSTSFPN
                     LRWLNFGGGISVPYEPGAAEFPIDDYGAELTRIADRLLRARDLTAILEPGRYLVAESG
                     TLLSQVTSLRISRGQWWIGVDTGFNHLVRPSKYGAYHHIVNATSGSSASLRQTFDLAA
                     MTEDVVVAGNLCESGDVFTRDEHGKTLTRRIDRTKIGDLLAFCDAGAYGFSMASLYNA
                     RLLPPEVMVDGDDVRLIRERQTYEDLVKGMR"
     gene            3106..>3246
                     /locus_tag="VNN25_21705"
     CDS             3106..>3246
                     /locus_tag="VNN25_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXH94207.1"
                     /translation="MQQYLTLVRHILDHGAPKADRTGTGTLSVFGYQMRFDLSAGFPL
                     LTT"
BASE COUNT          570 a         1008 c         1036 g          632 t
ORIGIN      
        1 attgttcttt tcctttgggg ggtggggagt tgggggtggg gggtgggaga gcgaacagtc
       61 gtttggttgc tctcccacac cccgctcccc acaccccaca ccccagctac ttcacgtatc
      121 cccgcccacg tagcacatgc cccggcaacg cattcgtcat cgtcccatcg cgaaggacag
      181 gaactccgtt cacgatcaca tccagcaccc cttcgctgaa gtgatgcgga tcttcgtacg
      241 ttgacacatc gcgaatattg ttcgcgtcga agacgacgac gtccgctttc atccctgggc
      301 gcagcacgcc gcggtcggtg aggttcgttc ggtcggcggc ttgcgacgtc gctttgcgca
      361 ccgcctcctc cagcgtgaag agatgctcgt cgcgcacgta gtgtcccatc acgcgcggga
      421 acgtgccgta ggcgcggggg tgtgatccgg cggatgtggc gcgggcgata ggtgggggag
      481 cgccggagtc cgatccgatc gagacgaacg attgcttcag agccgtctgc acatcgtctt
      541 cgttcatcga gaagaagatc acgccgggcg agccgtttgt ctccgcgaag aggcgcatca
      601 tggcctcctc gggcggcaca ttcatcatcg tcgcgatgtc ctcgacgaaa tgcttctcgt
      661 actgcacaag cgccggcgct ccgatacgcg tgatgtagat tcctttcggt ccgcgcttcg
      721 cgtactgcgc tttcagcgcc tcggcgatgc gggcgcgctg cgcgggatcg gcgagccgct
      781 tcttcagctc cgtgtagccg ccttcgagcg accagtcggg aacgagtgtc gatagagagg
      841 tcgacgacgc tgcgtatgga tagacattcg ccgcaacatc cagcccctcg gcgcgagcag
      901 cattgatacg ctcgatcaca tgcggcatgc gtccccagtt tgcgcggccg ccgactttga
      961 ggtgccagat gttgaccgga atcttcgcct cgcggccgat gcggaaggct tcatcgagcg
     1021 cggtgtcgat gtgatcgccc tcgtcgcgga tgtggctgaa atagacgccg ccatactgcg
     1081 ctgccacctt cgccaggttg atgatctcct cggtcgtcga gaacatcgca ggtagataga
     1141 tcagcgacgt cgacagtccg atcgcgccct cgcgcatggc ctgatcgacg atccgctcca
     1201 tctcgcgcat ctcgtccgcc gtcggcgcgc ggttcacggt gccgatgacc atctcgcgcg
     1261 gattgtcggc gccgacgaag agcgcgaagt tgattgcggt gccgctcttc tcgagaacgg
     1321 agaaccattc gccgagcgtg tgccacttcg gccggttgtt tgggctcgac acatcgatct
     1381 gcttctgatt gagcggacca ggtgagtgcc cttcgccggt cacctcggtc gtcacgccct
     1441 ggcgcacttt agcttccaag tgcgggtcct gaaacacgga gccctgcgat tgcccgagga
     1501 gatcgatgaa gccgggggag acgatgttgt cgtgtgcgtc gatcgttgcg gttgcattca
     1561 catgcgagag atcgccgatc gaaacgatcg tatcgccgcg cacgccaacg tcgccgcgat
     1621 accacggcgc accggtgccg tcgatgatgc gcccgttgat gatcttcaag tcgagagcga
     1681 gcggcttcgg ttgcgtcgca caggcggcga caaacagcgc taccgccgcg atcgcacttc
     1741 tgatcactga tctctggacg ctgatcactg ctttcatctc attcctttca cgaggtcttc
     1801 gtacgtttgc cgctcgcgga tcaaccgtac gtcatcaccg tccaccatca cctcgggagg
     1861 aaggagccgg gcgttgtaga gcgacgccat cgagaatccg taggcgccgg cgtcgcagaa
     1921 agcgaggaga tcgccgatct tcgtccgatc gattcttcgg gtgagggttt tgccatgttc
     1981 atccctcgtg aagacgtcgc ccgactcgca gagattgccg gcgacgacga catcctcagt
     2041 catcgctgcg agatcgaaag tctgccgcag cgacgcggac gaaccgctcg tggcgttgac
     2101 gatgtggtga tacgcgccgt acttcgaagg acgcacgagg tggttgaagc cggtgtcgac
     2161 gccgatccac cactgtccgc ggctgatgcg cagcgacgtg acttgtgaga gcagcgttcc
     2221 cgactccgcg acgaggtagc gtcccggctc cagaatcgcg gtcaggtcgc gcgcgcgcag
     2281 caagcggtcg gcgatgcgcg tcagctctgc gccgtagtcg tcgatgggaa actccgctgc
     2341 acccggctcg tacggcaccg agatcccgcc gccgaagttc aaccagcgca ggttcgggaa
     2401 cgaggtcgaa aggtcgagga gccggcgcgc cgactcgatg agcggcgcga tggtgtcgat
     2461 gcccgatccg atgtgcgcat ggagtccgac gaccttccgc ccgctgtcct cggcgatcat
     2521 gcgcgccgac tcgatctccg ccaggtcgat gccgaacttc acgccgccgc cagcggtcac
     2581 gacatcatgg tgatgtccgg cgccgatgtc ggtgttcacg cgcagcgcga tcggattccg
     2641 aaggtcgagc gcgagaatcc ggtcgatctc cgacatcgag ttgacgttga ttacgatccg
     2701 ctcgtcgccg atggcgcgaa ggtcgtcgtc ggaaacgttc gagcaagtga accagatgtt
     2761 gtcagccgtg tagccggcct tctgcgcgat gaagatctcg ccgggcgaca ccgtgtcgca
     2821 gccgaagccg gcgccgcgca tgaggtcgag gaccgccagc gcgccgttcg ctttcatcgc
     2881 gtaaaacggg cggaagggga gagcggtgaa ggctcgcttc acgcgctcga tctgtcgccg
     2941 gacgatctcc gcatcgtaga tgtagagcgg cgttccgaag cgcttggcca ggtcgatcgt
     3001 ggagacgttg ccgatgtgga gggtgtggtc gatgaaggag agcatgcgag cgcgcggatt
     3061 ataggcgcgg cgggactccg cgaattgacg aagttataat gagccatgca gcaatacctc
     3121 acgctcgtcc gccacattct cgatcacggc gcgccgaaag cggaccgcac cggcaccggc
     3181 acgttgagcg ttttcggcta ccagatgcgg ttcgacctca gcgctgggtt tccgctgctg
     3241 accacg
//
LOCUS       DATLVW010000745         8097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_484582, whole genome shotgun sequence.
ACCESSION   DATLVW010000745 DATLVW010000000
VERSION     DATLVW010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484582"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VNN25_21710"
     CDS             complement(<1..1607)
                     /locus_tag="VNN25_21710"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXH94208.1"
                     /translation="MFNNWKIGKRLGLAFGISILLVATVAVAGFWGLSQAVQTTYGIL
                     HHDAKMMEQASQAQANGLGLRRYEKDYFLNIGDKEKETAYTDKWNKEHADLVANIAEL
                     DKVSTLPEDKELLKVLQADFSTYTIGFQSVQTRINDNKIKTPTQANEAMTPFKDDIRR
                     LDTSLEDFAAKNVERMKAKEKVVADAESRTRSVMWVVFVVGFVLVSFMTVAVTRSITQ
                     PILSVVSIAEKLAEGQTDFEIEAGGKDETGSLLTAMKQMIDSNNEMVAAATGLAAGDL
                     SVRVRPRSDHDALGTALSNMIERLTEIIGEVRSGASSLTVASSQISASAQSLSQGTSE
                     QASSVEETTSSLEEMSASITQNAENSHQMESMAVKGTKDAEESAAAVKQSVDAMTKIA
                     EKISIIEEIAYQTNLLALNAAIEAARAGDHGRGFAVVATEVRKLAERSQAAAKDIGAL
                     ATSSVDVAQRSGRLLIELVPTIRKTADLVREVTAASIEQSGGVNQINRAMTLVDQVTQ
                     KNASASEELASTAEEMSSQAEALQQTIS"
     gene            complement(1836..2528)
                     /locus_tag="VNN25_21715"
     CDS             complement(1836..2528)
                     /locus_tag="VNN25_21715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH94209.1"
                     /translation="MVTHWKFGRHALAIALISIAGLGTASASTLPAETWRKAVNENAL
                     NLKKGDYTASLAAADHVLSEMVKYLGAGSAEDEMLATVLTQKALAQAGQGNISEALWY
                     WYIAQEISPAAAKSDLSAFGAPGEYLKRHPFAIADPNPGPGTTPAHVVKQVVPNFPSG
                     ASHFGAGDLIVHIIIDKNGTATLPHIVHPLPAPTLSYVALEALRRWQFAPAKQNGQAV
                     SFPFNLTVHYKL"
     gene            complement(2574..2747)
                     /locus_tag="VNN25_21720"
     CDS             complement(2574..2747)
                     /locus_tag="VNN25_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94210.1"
                     /translation="MRRLMRIFEHLLDRFLTMLNPRRTRKRKRVTMRYLMLLFLAAVF
                     ATPCIELIFKPMP"
     gene            complement(2744..4390)
                     /locus_tag="VNN25_21725"
     CDS             complement(2744..4390)
                     /locus_tag="VNN25_21725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXH94211.1"
                     /translation="MNAFPISDADRQQLIDAFVAETDDVLDEVEQLLLRLEQNPEEDQ
                     LLHEIFRGVHTLKGNAACLQFDELTRFAHVMEELLDGMREGRLDATTAHVSQLLDAAD
                     ALRDIAVRSVNGDGTLSERQEELLAQVTAWQANDDKASAPLSGNGAAAQEPARASRPS
                     RSLRVGIDKLDRMLNLAGEIATIRGTVRQLVEQSGNDEKTLDAVRELDRLSFDLQESI
                     MNVRLVPVGPSLRHFHRVVRDLASSQEKRVMLVLEGEDVEVDTTVIEQLKDPITHMIR
                     NAVDHGIELPSDRLGKGKNPIGTIRIAAAHDSGGIIVRFSDDGAGLNDAKIAERARAL
                     GLDPERMSRQDLWDLTLRPGFSTASQVTDLSGRGVGMDVVRRNVDAMRGTLSVHSEPG
                     AGTTVEIRLPLTVAVIEGFAVGVADETYIIPIDNVIECTEMPVGGNDATGVIDLRGEP
                     LPFLRIQRVFGLDASPHARENVVVVEHKGGRGRAGIVVDALFGSSQVVMKPMGRFLRQ
                     VDGVAGSSILGNGRVALILDVQQIINRAADAAAIQARNYA"
     gene            complement(4393..4893)
                     /locus_tag="VNN25_21730"
     CDS             complement(4393..4893)
                     /locus_tag="VNN25_21730"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXH94212.1"
                     /translation="MSAIEAGGELPIDEGDTMKRVYLYPGQIVTSGQPLMVSTILGSC
                     VSICLWDPAANIGGINHYLLPNNPLTGQSDLRYGNTATERLIEALLGLGVKKQRIVAK
                     IVGGASILTNFSDTRKSIGDQNVDIARTLLAKHGIDISGEQTGGTRGRKLLFHTGNGC
                     VFSKEI"
     gene            complement(4890..5744)
                     /locus_tag="VNN25_21735"
     CDS             complement(4890..5744)
                     /locus_tag="VNN25_21735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXH94213.1"
                     /translation="MSADEKNRSYVTVVREELQQYTQGLLRENEKLRAMATALESDKR
                     RLELEIIEARVVLDQQEELRRKTESFEAARAEMLTEVARLVQARDAATRELDLLRTRI
                     GDVERENETYAAQYHQIENQSSNLSNLYVASYQLHASVERETVLSTIQEIVVNLIGSE
                     EVAIFELDSDAREFRLASSFGVDHKRLKSFKAGSGPIGKCLVGGEVFVNDEVAGGEDK
                     LTACVPLKIGERVIGAILVFRLLEHKQSLQAVDHELFELLSIHASTALYCANLHNSVA
                     NTPLAVLG"
     gene            complement(5741..6133)
                     /locus_tag="VNN25_21740"
     CDS             complement(5741..6133)
                     /locus_tag="VNN25_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94214.1"
                     /translation="MNRKKILLVDDSSTILMMEKFILRNGPYDLVTAANGEEGVRKAA
                     EHLPDLILLDVIMPKMGGFEACRLIRSTESTKEIPIIMVTTRGEAANVEAGWANGCTD
                     YVTKPINSIELLAKVRNFLGDGASEVLQ"
     gene            complement(6148..6921)
                     /locus_tag="VNN25_21745"
     CDS             complement(6148..6921)
                     /locus_tag="VNN25_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH94215.1"
                     /translation="MTRHTLIDDILAPGGLSILFQPIFEIVNGDPVLFALEALSRGPK
                     GSNVECANVLFEYIRRKGRETEVDRVCIARAVAAAAGVPGAPAISINVHASTLERDPH
                     FAAFLSDICSKNGVPVSRVILEIVEQQKFFDEHAFFTALSELRALGVRIALDDIGIGY
                     SSHRMLIEVRPDLFKIDRYFIHGSAENEYSRAAIESIVLLATRLGGRVVAEGIEAAAD
                     FNTVTALGIDLVQGYYFARPSFPASFGAPSIHHESSATH"
     gene            complement(6918..7319)
                     /locus_tag="VNN25_21750"
     CDS             complement(6918..7319)
                     /locus_tag="VNN25_21750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94216.1"
                     /translation="MNPCTQSRPSPRMLLVEDEIVLLSALKKYFTREGYAVDCARELE
                     EAEALIATSFYDVVIADLRLSGTYAVEGLAILRFVRHNSRGTRVIILTAHTAPGIQRS
                     AHALGAEAFLPKPTPLAEIAACIDRLMAVPS"
     gene            complement(7583..7951)
                     /locus_tag="VNN25_21755"
     CDS             complement(7583..7951)
                     /locus_tag="VNN25_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94217.1"
                     /translation="MKTSAPGSKSFAPGGKDSARGGNDFAPGGNAFAPGGNDFAPGGN
                     EFARGGKSFPPGGKSFPPRANTFAPGANGFAPGGQSFAPVGKSFPPRANAFAPGAKSF
                     PPGGKDFGPALKSIARGVGA"
BASE COUNT         1418 a         2368 c         2595 g         1716 t
ORIGIN      
        1 gagatcgtct gctgcagcgc ctcggcctgc gaggacatct cttcagcagt tgaggccagc
       61 tcttcggatg ccgaggcgtt cttctgggtg acctgatcga cgagcgtcat ggcgcgattg
      121 atctgattga cgccaccgga ctgctcgatc gatgctgcgg ttacttcgcg aacgagatcg
      181 gcggtcttac ggatggtcgg cacgagctcg atgagcagcc ggcccgaacg ctgggcgacg
      241 tcgacgctgg atgttgccag cgctccgatg tcttttgcag cagcctggct gcgctcggcg
      301 agcttgcgaa cctcggttgc cacgacagcg aagccgcggc cgtgatcgcc ggcgcgggct
      361 gcttcgatag cggcgttcag cgcgagcaga ttggtctggt aggcgatctc ttcgatgatg
      421 gagatcttct cggcgatctt ggtcatggcg tcgacggatt gcttgacggc tgccgcgctc
      481 tcctcggcgt ccttggttcc cttgacggcc atcgattcca tctggtggct gttttcggcg
      541 ttctgggtga tcgaggcgct catctcttcg aggctggaag tggtctcttc gaccgacgaa
      601 gcctgctcgc tggtgccctg cgagagcgac tgcgcgcttg cggagatctg cgaggaggcg
      661 acggtgagcg aggaagcgcc gctgcggact tcgccgatga tctcggtgag ccgctcgatc
      721 atgttcgaga gggctgtgcc gagggcgtcg tggtccgagc gcggcctgac gcggacggaa
      781 agatctccgg ccgccagacc ggtggctgct gcgaccatct cgttgttcga gtcgatcatc
      841 tgtttcattg cggtgagcag ggagcccgtt tcatccttcc cgccggcttc gatctcgaag
      901 tcggtctgtc cctcggcgag tttctccgcg atgctgacca cgctgagaat cggctgcgtg
      961 atgctccggg tcacggcgac ggtcatgaac gagacaagaa cgaatccgac gacgaagacg
     1021 acccacatga cgctgcgcgt gcgagactct gcatcggcca cgaccttctc cttggccttc
     1081 atgcgctcca cgttcttggc tgcgaaatcc tcgagggacg tgtcgagccg gcggatgtcg
     1141 tctttgaacg gcgtcatcgc ttcattcgcc tgggtgggag ttttgatctt gttatcgtta
     1201 atccgggtct gcacggattg gaagccgatg gtgtacgtgg agaaatcggc ctgaagaacc
     1261 ttcaacagtt ctttgtcttc cggcagcgtg ctcactttgt cgagctccgc gatgttcgcg
     1321 accagatcgg cgtgctcctt gttccacttg tccgtgtagg cggtctcttt ttctttgtcg
     1381 ccgatgttga ggaagtaatc cttttcatag cggcgcagcc cgaggccgtt ggcttgggcc
     1441 tgggaggcct gctccatcat tttggcgtcg tggtgcagga ttccgtaggt ggtttggacc
     1501 gcctgcgaga gtccccagaa gccggctact gcgacggttg ctaccaggag gatgctgatc
     1561 ccgaaggcca gccccagtct ctttccgatt ttccagttgt tgaacatgat gttttcaatg
     1621 ctcctgatga aggttaggtc tgactacggt tgatggttga cgatgagaaa tgacttgtgt
     1681 gaacaccgag gatcgcaacg acgggaccag ttggcgcgaa gctgtggctt gtgagctaac
     1741 tagttgggta tgaacgactt gagaaagcaa cgcgaatctg cggagagcgt gcttttcgat
     1801 tattcgaaca cggttttcga gtatttgcgc agcggttaga gcttgtagtg caccgtgagg
     1861 ttgaagggga acgagacagc ctgtccgttc tgcttcgcag gcgcgaattg ccagcgcctc
     1921 aatgcctcga gcgcgacgta cgaaagcgtc ggcgcgggaa gcggatgaac gatgtgcggc
     1981 agcgttgccg tgccgttctt gtcgatgatg atatggacga tgagatcgcc cgcgccgaag
     2041 tgcgacgctc cggatggaaa gtttggtacc acctgcttga cgacgtgcgc cggcgtcgtg
     2101 ccgggtccgg ggtttgggtc ggcgatcgcg aacggatgcc gtttgagata ttcgccggga
     2161 gcgccaaagg ctgaaaggtc gctcttagcc gcggccggcg agatctcctg ggcgatatac
     2221 caataccaga gcgcctcact aatgttgccc tgtccggcct gtgcgagcgc tttttgcgtc
     2281 agcaccgtgg ccagcatttc atcctcggca ctgccagcgc cgaggtactt gaccatctct
     2341 gatagcacgt ggtcggcggc ggcgagcgag gccgtgtaat cgcccttctt gaggttgagg
     2401 gcattctcat tgactgcttt gcgccacgtt tcggcaggta atgtgctcgc cgaggctgtg
     2461 ccaagaccgg cgatcgagat cagcgcgatc gcaagggcgt gtcttccgaa tttccagtga
     2521 gtaaccatgt ttctctcctg ttggttctac ttcgttggaa tcgccggcgc gcattacggc
     2581 atcggcttga aaatcagttc aatgcagggc gtggcgaaaa cggcggcgag gaacagcagc
     2641 atcaagtacc gcatcgtcac gcgcttgcgc ttgcgggtgc gtctgggatt gagcatcgtc
     2701 agaaaccggt cgagaagatg ctcgaagatg cgcatcaggc gtctcatgcg tagttcctcg
     2761 cctggatggc cgcggcgtcc gcagcgcggt tgatgatctg ctgcacgtcg aggatcaggg
     2821 ctacgcgccc gtttccgagg attgacgatc cggccacgcc gtcgacctgc cgcaggaagc
     2881 ggcccatcgg cttcatgacg acctgactgc tgccgaagag cgcatcgacg acgatgccgg
     2941 cgcgtccgcg gccgcctttg tgctcgacaa caacaacgtt ctcccgcgca tgcggcgaag
     3001 cgtcgagtcc gaagacgcgc tggatacgaa ggaacggcag cggctcgccg cgaaggtcga
     3061 tgacaccggt ggcgtcgttg ccgcccacag gcatctccgt gcattcgatg acgttgtcga
     3121 tcgggatgat gtacgtctcg tctgcgacgc cgacggcgaa gccttcaatg acggccaccg
     3181 tcaacggtag tctgatctcg acggtcgtgc cggcgccagg ctcggagtga acgctgagcg
     3241 ttccgcgcat ggcgtcgacg ttgcggcgga cgacgtccat cccgacgccg cggccggaga
     3301 gatcggtgac ctggctggcc gtggagaatc cgggacgcag tgtcagatcc cagagatcct
     3361 ggcgcgacat ccgttcggga tcgaggccga gtgcacgggc gcgctcggcg atcttcgcgt
     3421 cgttcaatcc cgcgccgtcg tcggagaagc ggacgatgat gccgccggag tcgtgagccg
     3481 cggcgatgcg gatcgtgccg atgggattct ttcccttgcc gagacgatca ctcggcagtt
     3541 cgatgccgtg atcgacggcg ttgcggatca tgtgcgtgat cggatccttc aactgctcga
     3601 tgaccgtcgt gtcgacttcg acgtcttccc cttcgagcac gagcatcacg cgcttctcct
     3661 gggaagaggc gaggtcgcgg acgacgcggt gaaagtggcg cagcgacgga ccgaccggca
     3721 cgagccgcac gttcatgatc gattcctgca ggtcgaagct gagccggtcg agctcgcgca
     3781 cggcgtcgag ggtcttctcg tcgttgccgc tctgctcgac gagctgccgc accgtgccgc
     3841 ggatcgtggc gatctcgccg gcgaggttga gcatgcgatc gagcttgtcg atgccgacgc
     3901 gcaggctgcg tgacggacga gaggctcgag ccggttcttg tgcggccgcg ccgttgccgg
     3961 agagcggcgc gctcgctttg tcgtcgttgg cttgccatgc agtgacctgc gccagcaact
     4021 cctcctgccg ctcgctcaac gtgccgtcgc cgttgaccga gcgcaccgcg atgtcgcgca
     4081 gtgcatcggc cgcgtcgagc agttggctga cgtgcgcggt ggtggcgtcg agccgccctt
     4141 cccgcatccc gtccagcaac tcctccatga cgtgcgcaaa gcgcgtcagc tcgtcgaact
     4201 gcaagcacgc cgcattgcct ttgagcgtgt ggacgccgcg gaagatctca tgcagcaact
     4261 ggtcttcttc cgggttctgt tcgagacgca gcagaagctg ttcgacctcg tcgagcacgt
     4321 cgtcggtctc ggcgacgaac gcatcgatga gttgctggcg atcggcgtcg ctgatgggga
     4381 aggcgttcat cgttaaatct ccttgctgaa gacgcagccg ttgcctgtgt ggaagagaag
     4441 tttccggccg cgcgtgccgc ccgtctgctc gccgctgatg tcgatgccgt gtttggccag
     4501 gagcgtccgg gcgatgtcga cgttctggtc gccgatcgac ttgcgcgtat cgctgaagtt
     4561 ggtgaggatc gacgcgccgc cgacgatctt ggccacgatc cgttgcttct tcacaccgag
     4621 tcccaggagc gcctcgatca gccgttcggt cgccgtgttg ccgtagcgaa ggtcggactg
     4681 tccggtcagc gggttgttgg gcaacagata gtgattgatg ccaccgatgt tcgcggccgg
     4741 atcccagagg cagatcgaga cgcacgatcc gaggatggtg ctgaccatga gcggctgacc
     4801 cgacgtcacg atctgtcccg ggtagagata gacccgcttc atcgtgtcgc cttcgtcgat
     4861 cggcagttcg ccgccggctt cgatggcgct catcccagca ccgccaacgg cgtattggcc
     4921 accgagttgt gaaggttcgc gcagtagagt gcggtcgagg cgtggatcga cagtagctcg
     4981 aacagttcgt ggtcgaccgc ctggagcgac tgtttgtgct cgagcaggcg gaagacgagg
     5041 atcgcgccga tgacgcgttc gccgatcttc agaggcacgc acgcggtgag cttgtcttcg
     5101 ccgcccgcga cttcgtcgtt gacgaagacc tcgccgccga cgaggcattt gccgatcgga
     5161 ccgctgccgg ctttaaagct cttcagccgc ttatggtcga cgccgaagga ggaggcgagg
     5221 cggaactccc gggcgtcgct gtcgagctcg aagatagcca cttcttcgga gccgatgagg
     5281 ttgacgacga tctcctgaat cgtgctcagt accgtctcgc gctccacgct ggcgtgaagc
     5341 tggtacgagg cgacgtagag gttcgacagg ttgctgctct ggttttcgat ctggtggtac
     5401 tgcgcggcgt aggtctcgtt ctcgcgctcg acgtcgccga tgcgggtgcg cagcagatcg
     5461 agctcgcgtg tggcggcgtc gcgtgcctgc acgagcctcg cgacctcggt cagcatctcc
     5521 gcgcgtgctg cctcgaacga ttccgtcttc cggcgaagct cttcctgctg gtcgagaacg
     5581 acgcgcgcct cgatgatctc caactccagc cggcgtttgt cgctctccag cgccgtggcc
     5641 atggcccgca gcttttcgtt ctcgcgcaga aggccttgcg tgtactgctg cagctcctcg
     5701 cgtacgacgg tgacgtacga gcggttcttc tcgtccgcgc tcactggagc acctccgagg
     5761 caccatcgcc caggaagtta cggactttgg cgagcagctc gatcgagttg atcggcttgg
     5821 tcacgtagtc ggtgcagccg ttggcccacc cggcttcgac gttggccgct tcgccccgcg
     5881 tcgtgaccat gatgatgggg atttccttcg tgctctcggt gctgcggatc agacggcagg
     5941 cctcgaagcc gcccatcttc ggcatgatca cgtcgagaag aatgagatcg gggagatgtt
     6001 cggcagcctt gcgcactccc tcttcaccgt ttgcggcggt gacgagatcg taagggccgt
     6061 tcctcaggat gaacttctcc atcatgagga tcgtgctgga gtcatcgaca agcagaatct
     6121 tttttcggtt catgagcatt gctcctttca gtgagtcgcg gacgattcgt gatggatgga
     6181 cggggcaccg aacgatgccg ggaaagaggg acgcgcgaag tagtagccct gcacgaggtc
     6241 gatgccgagc gcggtgacgg tgttgaaatc ggcggctgcc tcgatgccct cggcgaccac
     6301 gcgcccgccg aggcgtgtgg caagcaggac gatcgattcg atggccgcgc gcgagtactc
     6361 gttctccgcg ctgccgtgga tgaagtagcg atcgatcttg aagaggtcag gtcggacttc
     6421 gatgagcatg cggtggctcg agtagccgat gccgatgtcg tcgagggcga tgcggacgcc
     6481 gagggcgcgc agttcgctga gtgcggtgaa gaacgcgtgc tcgtcaaaga acttctgctg
     6541 ctccacgatc tccagaatca cgcgcgagac cggcacgccg ttcttgctgc agatgtcgct
     6601 gagaaacgcg gcgaagtgcg gatcgcgctc gagcgtcgag gcgtgaacgt tgatcgagat
     6661 cgcaggcgcg cccggtactc ccgccgctgc cgcgaccgcg cgtgcaatgc atacacgatc
     6721 gacctcggtc tctctccctt tgcgccggat gtactcgaac aggacgttcg cgcattcgac
     6781 gttcgagcct ttcggtccgc gggacagcgc ttcgagtgcg aagagcacgg gatcgccgtt
     6841 cacgatctcg aagatcggct ggaagaggat cgacagaccg cccggcgcga ggatgtcgtc
     6901 gatcagcgtg tggcgcgtca cgatggcacc gccatcaagc ggtcgatgca ggccgcgatc
     6961 tctgcgagcg gtgtcggctt cggcaggaac gcctcggcgc ccagagcgtg cgcgctgcgc
     7021 tgaattccgg gcgccgtgtg cgcggtgagg atgatcaccc tcgtgccgcg ggagttgtgg
     7081 cgaacgaagc ggagaatcgc caggccttcg accgcgtagg tccccgagag acggagatcg
     7141 gcgatgacga cgtcgtagaa cgacgtagcg atcagcgctt ccgcttcttc cagctcacgt
     7201 gcgcaatcga ccgcatatcc ttcgcgagtg aagtacttct tgagagcgga gaggaggacg
     7261 atctcgtctt cgacgagcag catcctcgga ctgggacggg attgtgtgca agggttcatg
     7321 accgtcgtct atcgcaacgg cacggccacg tcctggcacg gagtcagcgc gccacgaacc
     7381 tccatcgttt caacggatta gcccgaacga tcgagattgg aaccggcacg gatttcaatt
     7441 attcaaacac gcttttcgaa tctttgggca gtggtgcttg tgcccagaga acgacgaatc
     7501 cgcagatgac gcagatggaa cgcagatgaa aaccgtcctc catccgcgat ctatctgcgt
     7561 catctgcgga caaggctctt catcacgccc ccacgccgcg agcaatactt ttcagtgcag
     7621 ggccaaaatc ttttcctccc ggaggaaaac tttttgctcc tggagcaaat gcatttgctc
     7681 taggagggaa actttttcct actggagcaa aactttgtcc tcccggagca aaaccatttg
     7741 ctcccggagc aaaagtattt gctcttggag ggaaactttt tcctcctgga ggaaaacttt
     7801 tccctccccg agcaaattca tttcctcctg gagcaaaatc atttcctccc ggagcaaatg
     7861 catttcctcc tggagcaaaa tcatttcctc cccgagcaga atcttttcct cctggagcaa
     7921 aactttttga tcccggagca gaagttttca ctgcaggagc agaagaactc tctctccgag
     7981 cagagccgaa gtggggtcag acccgaagtg gaagtgacgc aaattgacca ttgcatggcg
     8041 ctaagccaat tcccgtcact tccacttcgg gtctgacccc cagagctcac gcgaaga
//
LOCUS       DATLVW010000746        14781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_528111, whole genome shotgun sequence.
ACCESSION   DATLVW010000746 DATLVW010000000
VERSION     DATLVW010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528111"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="VNN25_21760"
     CDS             complement(<1..1938)
                     /locus_tag="VNN25_21760"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94218.1"
                     /translation="MKPLVIAASLLLCFSSTLAHAGSDLTLTISPSDAIVRAGFNRDY
                     GWIVHNNGPDPATNIIITIDNGGASATPSCAGGCVVPNIPAGQNGGLTQHVTYPLSAG
                     NVTMTGSVSSDSPDPNPSDNRATTTLTVSTDPDVRLSLVPSQDRLDLGLPFSLYILMA
                     NLSNSSAHDLDVTIDLSTDVGVPSLPAGCSTPSSGRITCHLDELAANANTGSPAWRLD
                     LTAPKAYGDGTLAFHASVTEREHDFDASSNTATATARLYQTIYVTTTADAGAGSLRQA
                     ILDANATCQGNGKLCAIGFLINEPSINPWKTIRVASPLPALTAISTGIDGAIQTALVG
                     DTNPRGPEIEISGNGMVDGDGIRLSGCETELANVVVNGFLRNGVSINAPPCGPPFLST
                     NVHVHDSFIGTDPTGSLARPNGERGIGVSSPLYSDGIGIANNVVSGNTLSGIFALSGT
                     VNVSNNRVGLKAHSDDPLPNGASGIFIGQFCGGSTVSNNIVAFNAQMGIAVATGARIV
                     DLPGNSTWSNGTLGIDVGLDGPSPAARPGGVNTPSITLVQYDAAKNKTIVEGDVQPPG
                     SIAFSKVEVFASDAPDPSGFGEGQRPLASQSLGSFADNTPLTHFRFELDGNLTGQWIS
                     ATDTLVHYNGFAKPAPEGTLSG"
     gene            complement(1961..3961)
                     /locus_tag="VNN25_21765"
     CDS             complement(1961..3961)
                     /locus_tag="VNN25_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94219.1"
                     /translation="MKRLTLFAALALSTTSLFASADLVTTISPPNALVRAGFTSQLFF
                     VVRNNGPETATGTTVAISSPVPYTCTCALGDIPSGQTRSGVLSFVAPSADTTLTISVT
                     ATSNVTDPLPANNAASVTLTVSTDPDVILRFNVPSRQDLDLPFTLPIFVGNNSNTTAH
                     DLEITIDFRTDVAVKSLPNGCSSLAAGRVVCRLDFLPPMTSVFAPTAAFVVQLVAPHD
                     YGSGSINFTGIVTEREHDFDPITNTYSQVTTLYRTYYVSTTKNDGGGSLRQAILDANA
                     SSQSPVAIAFRIAEASTTPWKTIRITAPLPVINGIRIEGATQTAFFGDTNPDGPEIEI
                     QGVNNLDSDGLTVTHCGCEIANLAINGFRRNGVSVIDAQSATCVPFTMPGLHNLFIGT
                     DPTGSEARPNGRGIGLSVLNGTSVFTAGGTTIIYDCVLSGNNMSGIFALSGSVDIWGN
                     RIGVKAHSDDPLPNGASGVFIGPGGYGSEIGPDQLTSNRAPNVIAFNREMGVAIAPGV
                     GDVSVRGNRIWSNGTLGIDIGLDGPTLSTTYPFDIPIAVPVLTLAHYDPVSKKTVIEG
                     DQDGQMIGGVRSFVVDLYANDAVDPSGFGEGQRPIARTQFADFTSPTHFRVAVDGDLT
                     GQFITAVATRGTIAGFAKPEPEGIDQLFLTQTSEFGRPIEVR"
     gene            complement(3962..5302)
                     /locus_tag="VNN25_21770"
     CDS             complement(3962..5302)
                     /locus_tag="VNN25_21770"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH94220.1"
                     /translation="MKRSLLLAIVLLSSSSLFASADSVPSTAQPANTFYVTESGNDGS
                     GSLRQAILDANAACTGTTPCTIAFRLTEPRHVGPWDTITVTTPLPVVTASKLLIDGGT
                     QTSFIGDTNVDGPEVEISGGGKVDGDGLLISGCDDQVMNLVVNGFGRNGISDSRTSCG
                     LFATTELSHLFIGTDPTGSQARPNGQRGIGISLGPGTSFFNDVQLVTIHDCVISGNTR
                     SGIFDLAGRVNIWDNRIGVKAHSDDPLPNGASGIFIGPGGYGSSIGPDVLRPNLAGNV
                     IAFNGETGVAVAPNVVNVSILGNKIWGNRLLGIDVGLDGPTLATKTFSGTTLLVPTLT
                     LAHYDPVSKKTIIEGDASAGGSLSLYANDTIDPSGYGQGQRPIGGTPLVPFSTITHYH
                     VEVDGDLTGQFITATSTGVDYEGFAKPGGVINSEGVDQGFLTQTSEFSRAIEVR"
     gene            5355..6422
                     /locus_tag="VNN25_21775"
     CDS             5355..6422
                     /locus_tag="VNN25_21775"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXH94221.1"
                     /translation="MTTTSSKKIAVIPGDGIGPEVIDAGLSVLNHVNDTRNLGLDFVH
                     FGWNADEYLRTGISIPPGGMDDIRNNYAAVYLGAFGDPRVPDMKHAADILLGMRFQLD
                     LYVNYRPVKLLNERLCPLKGKGRKDIDFVIFRENTEGAYVGMGGHFKKGTSEEIAIQE
                     DVSTRKGVERIIRHAFEFAKQTGRKSVCMSDKNNVMRFGGDLWLRTFEEVASEYPGIE
                     HFHMFVDALTMQMVRAPEMFEVIVTNNMFGDIVTDLGAALQGGLGMAASGNIHPGKVS
                     LFEPIHGSAPKYAGKDVANPIGAILTAAMMLEYLGNTAEADAIERAVVGAIEANETTH
                     DLGGTLGTKAAGEAVLRRVEL"
     gene            6654..7415
                     /locus_tag="VNN25_21780"
     CDS             6654..7415
                     /locus_tag="VNN25_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94222.1"
                     /translation="METVDGHELITRIPPEVADRLGYYVYIYSDPRDGKPFYVGKGQG
                     RRILAHLSVEVESRKMAKITELLDAGCEPQIDILAHALKDEETAFRVEAAVIDALGLD
                     QLTNEVRGWRSIQLGRMPLRELVIYYAAKPVDVQLPALLIRINRLFRHTMSSVALYEA
                     TRGIWKLGARRTNAEFAFAVFEGVVREVFKIDSWHRAGTTEYTTRPTDGWGNSTRWEF
                     SGRQADAEIRNQYVDRSVAAYFKKGQRSPVVYVNC"
     gene            complement(7717..7932)
                     /locus_tag="VNN25_21785"
     CDS             complement(7717..7932)
                     /locus_tag="VNN25_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94223.1"
                     /translation="MKKKPREDDLRPHYDFDYDKMKPNRFASEKKVYKQTFVVLDEDV
                     SKVFQSSEEVNILLRSAIRAMSIVKRN"
     gene            8081..10255
                     /locus_tag="VNN25_21790"
     CDS             8081..10255
                     /locus_tag="VNN25_21790"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HXH94224.1"
                     /translation="MKKLLIGILSFAAVSAFGAEGMWMPQQIPSLAAELKKAGLQIDA
                     KRFADLTGDPMGAVISLGGCSASFVSPDGLVVTNHHCGFGALQFNSTPQRDLITNGFL
                     AKTREEELPAGPGSRVFVTTNIEDVTARVSGSLAASLSNIDREKAIDRNVKQLVEECE
                     KPGGVRCRVASFFEGSQYLRTTQMEIRDVRLVYAPALGIGDFGGETDNWMWPRHTGDW
                     SYLRAYVGKDGRPADYSKDNVPYHPLHILKVSTEGVNPGDFVLVAGYPGRTFRYRTAA
                     EVSEMQSYSYPTTIKYATDLNNILRDLGKTNKAVEIANASRIKGNDNVLKNYTGTLTG
                     FNNFHIVGQRQKREADLAAYIVGHSDAAGKYKDVMNQMNAIHQQSIANEQRDTVLSWI
                     LRSSPMLGQSITLYRLSMEKAKASDLDREGGFQQRDWPRITEGITRAQKGIEPNSDRA
                     GLRYFLNESQKLPAGQRIAPIDAAITKAGGVEPFLDQLYANTKVADLAQRKAMQDETT
                     AQLVARNDSMIDLVAAIMPLSLENEHNQKELSGAMSKIRPMYMDALRASNGGRLYPDA
                     NGTLRVTFGQVKGYTPRDGVMYTPKTTVGGVLAKNTGTGEFDAPKAELDAIRNNKTAG
                     YVDPKIGGVPVDFLSTVDTTGGNSGSPTLNAKGELCGLLFDGTYESLGSDFVVDENIT
                     RSIHVDAVYMLWVMDAVDGAHNLLREMNVPVHFGGGAVGAGK"
     gene            complement(10325..10612)
                     /locus_tag="VNN25_21795"
     CDS             complement(10325..10612)
                     /locus_tag="VNN25_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94225.1"
                     /translation="MAYGVVHFFPGGTKEQYEASLAAVHPDRSTLPKGQIYHAAGPSA
                     GGWTIVAIHDSKASWEAFRDNTLGPKLQAGVEGGFTNPPQEQSFEVYNLQK"
     gene            complement(10736..10939)
                     /locus_tag="VNN25_21800"
     CDS             complement(10736..10939)
                     /locus_tag="VNN25_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94226.1"
                     /translation="MATPEFPLRVIFDDGEVEEIESAEELMERVETIDSTDPGNRIWI
                     RDVLDRTVLLRMRNGMVEVFEAV"
     gene            11052..11795
                     /locus_tag="VNN25_21805"
     CDS             11052..11795
                     /locus_tag="VNN25_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXH94227.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGKIFTRIIKEMTVAARMGGGDVD
                     GNPRLRAAVAEAKASNMPKDNIDRAIKRGTGELEGVNYEELTYEGYGPGGVAVMVETM
                     TDNTNRTTPEIRHAFEKCGGNMGTPGSVRFQFERKGYFAVEKSAATEDRLMEVALEAG
                     ADDLQTLDADIYEIYTAPDAFEQVRAALEKNKVPTVEAKIGQIPANLVKLDDQKSKQM
                     MRLLEMLDDQDDVQNVWTNFDIPEEMMEE"
     gene            11940..12845
                     /gene="prs"
                     /locus_tag="VNN25_21810"
     CDS             11940..12845
                     /gene="prs"
                     /locus_tag="VNN25_21810"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HXH94228.1"
                     /translation="MPLIFSIAAYDYMADALVRACGAERGVVERSNFPDGEHYLRVAS
                     GVLDQHVVAIGGTVSDAAMLELYDVACALVENGAETLTLIVPWFGGATMDRASKSGES
                     VTAKNRARIFSSIPPAKNGNQILLLDLHTEGLPHYFEGSLHPVHVYARPVIEQLARDL
                     GGASFILGSTDAGRAKWVESLANRLGVPAAFVFKRRLGPAETEVTAVSTKLDGETVVI
                     YDDMIRTGSSLMNAARAYRDAGAGRLVAIATHGVFPNGAFETIQATGLFGAIACTDSH
                     PNALALSARGLIVRPVAELFCRYLR"
     gene            complement(12921..14534)
                     /locus_tag="VNN25_21815"
     CDS             complement(12921..14534)
                     /locus_tag="VNN25_21815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH94229.1"
                     /translation="MNRRLTLLASALLLVSSQAFAVHHEVTTVEVVQVPVYISVNGHA
                     VTGLTKDDFEIYVNGKPQPVDYFDTIDFAALPASTTSDPRQRRLYLLTFDERFSSRAA
                     LQRAQKAALQYVDHALPGDVFGVATYTGNHGLQFVVPFTRDQVLLRRALRGLSSAQAG
                     DPLFLTVTTGGGSMAGLDTPGMQDVGFRRELSIQPLRYAVEDEIRALSAVADRMAALD
                     GQKHVVLLSGGFDASLIHGVQSVHPTTHTGYGTFGPTGYGTFSMLHGGTNAFATPYEN
                     SMSRVRYNDVAVTGNPQLGFDPRLGAYMQEMFGHFTAASVFLDAIAIEGLRPRQRAID
                     TEGLFALTHDTGGTLVEHTSDMNVAIQELTDKQRVVYVLGFRPTSERKTNGIRVKVRN
                     APSGARVTYRPSYSNEISLPASNDGLHLADVLMNDLPQTGLTLRAAVTPSGKHAVVDV
                     DIATRELLALSDSQDVEAEALVYVYSGNAVVAFKERRITIEAARVDPAKPVRLSENFE
                     LPDGHYAAKVLVRFGDQIGFVRSDFVVGQ"
BASE COUNT         2915 a         4473 c         4624 g         2769 t
ORIGIN      
        1 cccggagagg gtgccttccg gtgccggctt cgcgaagccg ttgtagtgga ccaacgtatc
       61 ggttgccgag atccactggc cggtcagatt gccgtcgagc tcgaatcgaa agtgggtgag
      121 gggtgtgttg tctgcaaacg atcccagcga ttgcgatgcc agtggccgct gaccttcgcc
      181 gaagcccgag ggatctggcg catcgctggc gaagacttcc accttggaaa aggcgataga
      241 gccgggcggc tggacgtcac cttcgacgat cgtcttgttt ttggcggcgt cgtattggac
      301 aagcgtgatg gacggcgtgt tcactccgcc aggcctggca gccggcgacg ggccatccag
      361 accgacgtcg atcccgaggg tgccattgga ccaggtggag tttccgggca gatcgacgat
      421 acgcgcaccg gtggcgactg cgatgcccat ctgcgcgttg aacgccacga tgttgttcga
      481 gaccgtggag ccgccgcaga actggccgat gaagatgccg ctcgcgccat tcggcagcgg
      541 atcgtcgcta tgcgctttca atccgacgcg gttgttcgac acgttgaccg tgcccgagag
      601 cgcgaagatg ccgctcaacg tgttgccgga gacgacgttg ttcgcgatgc cgatcccgtc
      661 gctgtagagg gggctggaga cgccgatgcc gcgctcgccg ttcggacgcg ccagcgatcc
      721 cgtcggatcg gtgccgatga acgagtcatg aacgtgaacg ttcgtcgata gaaagggtgg
      781 accgcatggc ggcgcattta tcgatacccc gttcctgaga aacccattca cgacgacgtt
      841 cgccagctcg gtctcgcagc cggagagccg gattccgtcg ccgtcgacca tcccgttgcc
      901 gctgatctcg atctccggcc ccctcgggtt cgtgtcgccc acgagcgccg tttggatggc
      961 gccgtcgatg cccgtgctga tcgccgtcag cgccggaagg ggcgaggcga cgcggatcgt
     1021 cttccacgga ttgatcgacg gttcgttgat gaggaatccg atcgcgcaca actttccatt
     1081 tccctggcag gtggcattcg cgtcgagaat cgcttgccgc aatgagcccg cgccggcgtc
     1141 ggctgttgtg gtgacgtaga tggtctggta gaggcgcgcc gttgccgtgg ctgtgttcga
     1201 tgaggcatcg aaatcatgct cgcgctccgt cacagaggcg tggaaagcaa gcgtgccgtc
     1261 gccgtaggcc tttggtgcgg tcaggtcgag cctccacgcg ggagagccgg tgttcgcgtt
     1321 tgctgcgagc tcatcgagat ggcaggtgat ccgtccggac gacggcgtgc tgcaacctgc
     1381 gggaagcgag ggcacaccga catcggtgct gaggtcgatc gtgacatcga gatcgtgcgc
     1441 ggaggaattc gaaagattgg ccatgaggat gtagagcgaa aacggaagcc cgaggtcgag
     1501 ccggtcttga gagggaacga gtgacagtct gacgtctgga tccgtggata ccgtgagtgt
     1561 tgtcgtggcg cgattgtccg acggattcgg gtcaggcgaa tcactgctca ccgatcctgt
     1621 catcgtcacg ttgcccgcgc tgagcggata ggtgacgtgt tgtgtgaggc ctccgttctg
     1681 accggcggga atgttgggaa cgacgcagcc acccgcacac gacggagtgg cgctggcgcc
     1741 gccgttatca atggtgatga tgatgttggt ggcgggatcg ggcccgttgt tgtggacgat
     1801 ccaaccgtaa tcgcgattaa acccggcgcg aacgatggcg tccgaagggg aaatcgtcaa
     1861 cgtcagatcc gagccagcgt gggccagtgt cgaggagaag cagaggagga gcgaagcagc
     1921 gatgacgagg ggtttcattc tggggtttct attggggaga tcaccgtact tcgatgggcc
     1981 ggccgaactc ggatgtctgg gttaagaaga gctgatcgat cccttcaggt tcaggtttgg
     2041 cgaagccggc gatggtgcct cgggtcgcca ccgccgtgat gaactggccg gtcaggtcgc
     2101 cgtcgacggc tacgcggaag tgtgtgggtg acgtgaaatc agcgaactgc gttcgcgcga
     2161 tcggtcgctg tccttcgccg aatccggagg gatcgacagc gtcattcgca tagaggtcga
     2221 cgacgaagga gcgcactcca ccgatcatct gaccgtcctg gtctccctcg atcaccgtct
     2281 tctttgacac cgggtcgtag tgcgccaaag taagcaccgg tacggcgatc gggatatcga
     2341 acggatacgt ggtcgaaagc gttggaccgt cgagaccgat gtcgattccg agcgtgccgt
     2401 tgctccagat tctgttgccg cgcacggaga cgtcgccgac gccgggcgca atggccacgc
     2461 ccatctcgcg attaaacgcg atgacgtttg gtgcgcggtt agacgtcagc tggtcaggtc
     2521 cgatctccga tccgtagccg cctgggccga tgaagactcc gctggcgccg ttcggcaacg
     2581 gatcgtcgct gtgcgctttc acgccgatgc gattgcccca gatgtcgaca ctcccgctga
     2641 gggcgaagat gccgctcatg ttgttgccgg agaggacgca gtcgtagatg atggtcgttc
     2701 cgccagcagt gaaaacactc gtcccgttca acaccgacaa cccgatcccg cgcccattcg
     2761 gccgggcctc ggaaccggtc gggtcggtcc cgatgaagag attgtggaga ccgggcatcg
     2821 tgaacggaac gcacgttgct gattgcgcat cgatgacgga aacgccgttg cgccggaatc
     2881 cgttaatggc aagattcgcg atctcgcagc cgcaatgcgt gactgtcaga ccgtcgctgt
     2941 cgaggttgtt gactccctgg atctcgatct caggcccatc cggattcgtg tctccaaaga
     3001 aggcggtctg cgtcgcgcct tcgatgcgaa ttccattgat gacaggcagc ggcgccgtga
     3061 tccggattgt tttccacggt gtcgtcgacg cttcggcgat ccggaacgca atcgcgaccg
     3121 gactttgcga tgaggcattg gcgtccagaa tggcctgccg cagcgagccg ccgccgtcgt
     3181 tctttgtggt cgagacgtaa tacgtccgat agagcgtcgt cacctgcgag taggtgttcg
     3241 tgatcggatc gaagtcgtgt tcgcgctcgg tgacgattcc ggtaaaattg atcgatccgg
     3301 agccatagtc gtgcggtgcg acaagctgga caacgaacgc cgccgtcggc gcaaacactg
     3361 acgtcatagg aggcaggaag tcgagccggc agacgacacg tcccgcggcc aaactgctgc
     3421 aaccgttcgg cagcgacttc acagccacat ccgtccgaaa atcgatcgtg atctcgagat
     3481 cgtgggcggt cgtattggag ttgttgccga cgaaaatggg aagcgtgaat ggaagatcga
     3541 gatcctgcct ggacggaaca ttgaatctga gaatgacgtc cgggtcggtc gagacggtca
     3601 acgtcaccga agcggcgttg ttggcgggaa gcggatcggt cacgttgctt gtggccgtga
     3661 ctgagatcgt gagcgtcgtg tctgccgagg gcgccacgaa ggagagcact ccgctgcgcg
     3721 tctggccgga tggaatgtcg ccaagcgcgc aagtgcacgt gtaaggaacg ggcgaggaga
     3781 tggcgaccgt cgtgccggtc gcggtctccg gtccgttgtt acgcacgacg aagaaaagct
     3841 gcgaggtgaa tccggcgcgg acgagtgcgt tcgggggtga gatcgtcgtg acgagatcgg
     3901 cggacgcgaa cagggacgtg gtgctgagtg cgagtgctgc gaaaagcgtg agcctcttca
     3961 tttatcgcac ctcgatcgct cggctgaact ccgaggtctg ggtgaggaag ccttgatcga
     4021 ctccctctga gttgatgact ccgccgggct tggcgaagcc ctcatagtcc actcccgtgg
     4081 acgtggcggt aatgaattgt cctgtcaggt cgccatcaac ctcgacgtga tagtgagtga
     4141 tggtggagaa gggaaccagc ggggtgccgc cgatcggacg ctgtccctgt ccgtaaccgg
     4201 atggatcgat cgtatcgttg gcgtagagac tcagtgatcc gcccgctgag gcgtcgcctt
     4261 cgatgatcgt cttcttcgag acaggatcgt agtgtgccag cgtcagcgtc gggacgagaa
     4321 gcgtcgtgcc ggagaatgtc ttcgtcgcga gcgtcggtcc gtctagaccg acgtcgattc
     4381 ccaggagccg gttgccccag atcttgttgc cgaggatcga gacattgacg acgttggggg
     4441 cgacggcgac gccggtctct ccgttgaatg cgatgacgtt tccggcgagg ttcggcctca
     4501 gaacatcggg accgatggag gatccgtatc cacccggccc gatgaagatg ccgctggcgc
     4561 cgttcggcag cggatcgtcg ctgtgcgcct tcactccaat ccggttgtcc cagatgttga
     4621 cccgtcccgc gaggtcgaaa atgccgctgc gcgtattgcc cgagatgacg cagtcgtgaa
     4681 tggtcaccaa ctggacgtcg ttgaagaagc tcgttcctgg gccgagcgag atgccgatgc
     4741 cgcgctgtcc gttcggccgc gcctgcgatc cggtgggatc cgtgccgatg aagagatgac
     4801 tcagctccgt ggtcgcgaag aggccgcagg aagtccgtga gtcggaaatg ccgttgcggc
     4861 cgaacccatt gacgacgagg ttcatgacct gatcgtcgca gccggagatg agcagtccgt
     4921 cgccatcgac tttcccgccg ccgctgatct cgacctcggg gccatcgacg ttcgtgtcgc
     4981 cgatgaaact ggtctgcgtg ccgccgtcga tcaggagttt cgatgcggta actaccggca
     5041 gaggtgtggt gaccgtaatg gtgtcccacg gtccgacatg acgcggctcg gtgagccgga
     5101 acgcgatcgt gcacggcgtc gtgccggtgc aagctgcgtt tgcatcgaga atggcctgac
     5161 gcagcgagcc gctgccgtcg ttgccggatt ccgtgacgta gaaggtgtta gccggttgcg
     5221 ctgtggatgg gacgctgtct gcggaggcga agagcgagga tgaagaaaga aggacaatcg
     5281 ccaagaggag agatctcttc atgcgaataa gtgtaacgga cgccgcgaag catccgtcta
     5341 taatccgccg ccgcatgacg acaacaagct cgaagaagat cgcagtaatc cccggtgacg
     5401 ggatcggtcc cgaagtcatc gacgccggcc tcagcgtcct caaccacgtc aacgacaccc
     5461 gcaacctcgg cctcgacttc gttcacttcg gctggaatgc ggacgagtac ctccgcaccg
     5521 gcatctccat cccgcccggc ggcatggacg acatccgcaa caactacgcg gccgtctacc
     5581 tcggcgcatt cggcgatccg cgcgtccccg acatgaagca cgccgccgac atcctcctcg
     5641 gcatgcgctt ccagctcgat ctctacgtca actaccggcc ggtgaagctg ctcaacgaga
     5701 ggctctgtcc gctcaaaggc aagggccgca aggacatcga cttcgtcatc ttccgcgaga
     5761 acacggaagg cgcgtacgtc ggcatgggcg gccacttcaa gaaagggacg agcgaagaga
     5821 tcgccatcca ggaggatgtc tccacgcgca aaggcgtcga gcgcatcatc cgccacgcct
     5881 tcgagttcgc gaagcagacg ggacgcaagt cggtgtgcat gtcggacaaa aacaacgtca
     5941 tgcgcttcgg cggcgatctc tggctgcgaa cgttcgaaga ggtggcttcg gagtatcccg
     6001 gcatcgagca cttccacatg ttcgtcgacg cgctgacgat gcagatggtg cgcgcgccgg
     6061 agatgttcga ggtgatcgtg accaacaaca tgttcggcga catcgtcacc gacctcggcg
     6121 ccgcactcca gggcggcctc ggcatggccg cctcgggcaa catccatccc ggcaaagtct
     6181 cgctcttcga gccaatccac ggctccgcgc cgaagtacgc aggaaaggac gtagccaacc
     6241 caatcggcgc catcctcacc gccgccatga tgctcgaata cctcggcaac accgccgaag
     6301 cggacgccat cgagcgtgcc gtcgtcggcg caatcgaagc caacgaaacc acccacgacc
     6361 tcggcggcac cctcggcaca aaggcggccg gggaggcggt attgcggagg gtggagttgt
     6421 agaataaccg catgccgatc tggaccgtcg ctctagtcgt ctccgcctgc ggtgccttgg
     6481 gaggcttcgt gaatgcgttt cttaccgggc accttcatct gcctcgccgc acgtctgata
     6541 cgtactctcc tgccgaggtc gctgcgtcca tcgcgctagg gatcggcgcg gcacgtctcc
     6601 tggcgacaga gccgaaaaga accggtcatg gatcagagct gcggtgacga cccatggaaa
     6661 ccgtcgatgg gcatgaactg atcacccgga taccaccgga ggtagctgat cgcctcggct
     6721 actacgttta catctacagc gatccacgcg acggaaagcc gttctacgtc gggaagggac
     6781 aaggcaggcg gattctggcg cacctctctg ttgaggtgga gtcgcggaag atggcgaaga
     6841 tcacggagtt gcttgacgcc ggttgtgagc cgcagatcga tatccttgcg cacgcgctca
     6901 aagacgaaga gacggcgttc agggtcgagg ccgcggtcat tgatgcgctc gggctcgatc
     6961 agctcacgaa tgaggtgcgc ggatggagaa gcattcagct cggacgaatg cctcttcgtg
     7021 agctggttat ctactacgcc gcgaaaccag ttgatgtgca actgccagct ctgctgatcc
     7081 gaatcaatcg gctcttccgc cacacgatgt cctcggtggc gctctatgag gcgacccgtg
     7141 gcatatggaa actcggcgct cgccgaacga acgccgaatt tgcctttgcc gtgttcgaag
     7201 gtgtcgtgcg cgaagtcttc aagatcgact cgtggcaccg ggcgggaacc acggagtaca
     7261 cgacgcgtcc caccgacggt tgggggaact caacgcgatg ggagttcagc ggacggcagg
     7321 ctgatgccga gatccgaaac cagtatgtgg atcgctccgt cgctgcgtac ttcaagaagg
     7381 gacagcgaag cccagtggtc tacgtgaatt gctagcttgt cttagttcgt gtctgtttca
     7441 gtctctggtt acttgcgctc caaccgccaa tcgagcgcct tctcccctcg tgcttttcga
     7501 ggggggaagg tgccgaaggc ggatgagggg tgtcgtgccg tcgaacagcg tgaagttctt
     7561 gcccgtctga atcttcgcca gtcgccgcaa gagtccacgg cccctcatcc ggccttcggc
     7621 caccttctcc ccatctgaaa agcgatgggg agaaggcgct cgattggggt agcagcgcaa
     7681 gagatcaagg ttgaaacagg gccagcaacg aatcagtcag ttgcgcttca cgatagacat
     7741 cgcccggatc gccgatcgca gcaagatgtt cacctcttcg gatgactgaa agactttcga
     7801 gacatcttcg tcgagaacga cgaacgtctg cttgtacact ttcttctcac tcgcgaatcg
     7861 attcggcttc atcttgtcgt aatcgaaatc gtagtgaggc ctgaggtcgt cttctctggg
     7921 tttcttcttc atgatgtcgc gagcaggatc gttcggctgg ttctccccgt caagccggga
     7981 attggcgttg gcgatatgct gtccagagag actgataccc gaggctgcag cgcgcgtctc
     8041 gttccatttg aaacccaacg aaacaaccta aggagaacgt gtgaagaaac tcttgatcgg
     8101 aatcctcagc tttgccgcgg tgtcggcatt cggtgctgaa gggatgtgga tgccgcagca
     8161 gattccgtcg ctggcggcgg aactgaagaa ggctggactg cagatcgatg cgaagcgctt
     8221 tgctgatctg acaggcgacc cgatgggcgc ggtcatctcg ctcggcggct gcagcgcatc
     8281 gttcgtctcg cccgacggcc tcgtggtgac gaatcaccac tgcggcttcg gagcgttgca
     8341 gttcaactcc accccgcagc gcgacctcat caccaacggc tttctcgcca agacgcgcga
     8401 ggaagagctc ccggctggtc cgggatcgcg cgtattcgtc acgacgaaca tcgaagacgt
     8461 cacagcgcgc gtctccggca gtctggccgc ctcgctctcg aacatcgacc gagagaaagc
     8521 catcgaccgg aacgtcaagc agctcgtcga ggaatgcgag aagccgggcg gcgtgcgctg
     8581 ccgtgtggcc tcgttcttcg aaggctcgca gtatctccgc accacgcaga tggagatccg
     8641 cgatgtccgt ctcgtctacg ctcccgcgct cggcatcggc gacttcggcg gcgagaccga
     8701 caactggatg tggccgcgtc acaccggcga ctggtcgtat ctccgcgcct acgttggcaa
     8761 agacggcaga cccgccgact actcgaagga caacgttccc tatcatccgc ttcacatcct
     8821 caaagtatcg actgaggggg tgaatccggg cgatttcgtc ctggtggctg gttatcccgg
     8881 acgcaccttc cgctatcgca ccgccgcgga agtcagcgag atgcagagct actcgtatcc
     8941 gacgacgatc aagtacgcca cggatctcaa caacatcctc cgcgacctcg gcaagaccaa
     9001 caaggccgtc gagatcgcca acgccagccg catcaaaggg aacgacaacg tcctcaagaa
     9061 ctacaccggc acactcaccg gcttcaacaa cttccacatc gtcggtcagc gccagaagcg
     9121 cgaagcggac cttgccgcgt acatcgtcgg ccattccgac gcggccggta agtacaaaga
     9181 cgtgatgaat cagatgaatg cgattcatca gcaatcaatc gccaatgagc agcgcgacac
     9241 ggtcctgagc tggatactcc gctcctcgcc gatgcttgga caatcgatca cgctctatcg
     9301 cctctcgatg gagaaagcga aggcctccga tctcgatcgc gaaggcggat tccagcagcg
     9361 cgattggccg cgcatcaccg agggcatcac gcgcgcgcag aaaggcatcg agccgaacag
     9421 cgaccgcgcc ggtctgcgct acttcctcaa cgaatcacag aagctgccgg cgggacaacg
     9481 catcgcgccg atcgatgccg cgatcaccaa agcaggcggc gtcgagccgt tcctcgatca
     9541 gctctacgcg aacaccaaag ttgccgacct cgcccagcgc aaagcaatgc aggatgagac
     9601 gaccgcgcag ctcgtggccc gcaacgactc catgatcgac ctcgtcgccg cgatcatgcc
     9661 gctctccctc gagaacgagc acaaccagaa ggagctcagc ggcgcgatgt cgaagatccg
     9721 tccgatgtac atggacgcac tccgcgcctc gaatggcggc cggctctacc ccgacgccaa
     9781 cggcacgtta cgcgtcacct tcggtcaggt gaagggctac accccacgcg acggcgtcat
     9841 gtacacaccg aagaccacgg tcggcggcgt cctggcgaag aacaccggga ccggcgagtt
     9901 cgacgccccg aaagcggagc tcgacgccat ccgcaacaac aagacggctg gctacgtcga
     9961 tccgaagatc ggcggcgttc cggtcgactt cctatcgacg gtcgacacca ccggcggcaa
    10021 ctccggctcg ccgacactca acgcgaaggg cgagttgtgc ggtctgctct tcgacggcac
    10081 ctacgagtcg ctcggatccg acttcgtcgt cgacgagaac atcacgcgct cgattcacgt
    10141 cgacgcggtc tacatgctct gggtcatgga cgccgtcgac ggcgctcaca acctcctgcg
    10201 cgaaatgaac gtgccggtgc acttcggcgg cggcgcggta ggagcaggga aataagctca
    10261 cttcatcgat gaaccaaggg cgggctctcg ggcccgccct tttgttctgc cgcagcccgt
    10321 ctcttcactt ctgaaggttg tacacctcga aggactgctc ctgaggtggg ttcgtgaagc
    10381 cgccttctac gccggcctga agcttcggac cgagcgtgtt gtcacgaaac gcttcccaac
    10441 tcgctttcga gtcatggatc gctacgatcg tccatccgcc cgccgagggg cctgccgcgt
    10501 gatagatctg ccctttcggg agggtgcttc tgtctgggtg taccgctgcc agtgacgcct
    10561 cgtactgctc tttggtcccg ccgggaaaga agtgaactac tccataagcc atactctctc
    10621 tccttttcgt gacgcgtagt acgcgtcaaa tcacgcgaca ggatacgccg aaggcgtgta
    10681 gcgccgccgg cccggcggct gtaccgccgg cggctcgccg gcgacactcg agacgtcaca
    10741 ccgcctcgaa cacctccacc atcccatttc gcatccgcag caacaccgtg cgatcgagga
    10801 cgtcgcgaat ccagatgcga tttccaggat cggtggagtc gatcgtctcc accctctcca
    10861 tcagttcctc agcggactcg atctcctcga cctcaccgtc gtcgaagatg acacgaagcg
    10921 ggaattccgg agtggccatg agctcttcga acgcgcgcga cgggagctta gcgcttcggc
    10981 tatactcccc gcctcaactt ttcgagacca gtggcgcaag cccgtctcgc ctcaaccaaa
    11041 ggagcattcc gatgtccggc cacagcaaat ggagcaccat caaacacaag aaaggtgcgg
    11101 ccgacgccaa gcgcggaaag atcttcaccc gcatcatcaa ggaaatgacc gttgccgcgc
    11161 gcatgggcgg tggcgacgtc gacggcaatc cccgcctgcg ggctgcggtg gccgaggcga
    11221 aggccagcaa catgccgaaa gacaacatcg accgcgccat caagcgcggc accggtgagc
    11281 tcgaaggcgt gaactacgaa gagctcacgt acgaaggtta cggtcccggc ggcgtagccg
    11341 tcatggtcga gacgatgacc gacaacacga accggaccac tcccgagatc cgccacgcct
    11401 tcgaaaagtg cggcggcaac atgggaacgc ccggctcggt ccgtttccag ttcgagcgca
    11461 agggttactt cgcggtcgag aaaagcgcgg ccacggaaga ccggctgatg gaagtggcgc
    11521 tcgaagccgg cgctgacgac ctgcagacgc tcgacgccga catctacgag atctacacgg
    11581 cgcccgacgc gttcgagcaa gttcgcgcag cgctggagaa gaacaaggtt cctacggtcg
    11641 aagccaagat cggccagatc ccggccaacc tggtcaagct cgacgaccag aagtcgaagc
    11701 agatgatgcg cctcctggag atgctcgacg accaggacga cgtgcagaac gtctggacga
    11761 acttcgatat ccctgaagag atgatggaag agtagggaag cagagatcag gggatcagag
    11821 ataagagatc agaggagtag agatcagaga gttagtagat cgatcggtag actctgatct
    11881 ctgatctctt ctgtctctga tctctgtagt ccccacgaat ccgttaatct actcactcct
    11941 tgcccctcat cttctccatc gccgcctacg actacatggc cgacgcgctc gtgcgcgcct
    12001 gcggcgctga gcgtggcgtc gtcgagcgga gcaacttccc ggatggcgaa cactatctgc
    12061 gcgttgcctc gggcgttctc gatcagcacg tcgtcgccat cggcggaacg gtctccgatg
    12121 cggccatgct cgagctgtac gacgtcgcat gtgcgctcgt cgagaatggc gccgagacgc
    12181 tgacgttgat cgtgccgtgg ttcggtggcg cgacgatgga ccgggcatcg aagagcggcg
    12241 agtcggtgac ggccaagaat cgggcgcgca tcttctcgtc gattccgccc gcgaagaacg
    12301 gcaatcagat cctgctgctc gatctgcaca ccgaggggct tccgcactat ttcgaaggaa
    12361 gcctgcatcc ggtgcatgtc tacgcccgtc cggtcatcga gcaactcgcg cgcgacctcg
    12421 gcggcgcctc cttcatcctc ggctccaccg acgccggccg cgccaagtgg gtggaatcgc
    12481 tcgccaaccg cctcggcgtt ccggccgcct tcgtcttcaa gcgccgcctc gggcccgcgg
    12541 agaccgaggt gactgcggtc agcacgaagc tcgacggcga gacggtcgtc atctacgacg
    12601 acatgatccg caccggtagc tcgctgatga acgccgcacg cgcgtatcgc gacgctggtg
    12661 ccggccgtct cgtcgccatc gccacgcacg gagtcttccc gaacggtgca ttcgagacga
    12721 tccaggcgac cggtctcttc ggcgccatcg cctgcaccga cagccatccg aacgcgctcg
    12781 cactcagcgc acgaggtctc atcgtccggc ccgtcgcaga gttgttctgc cggtatttgc
    12841 ggtgagtttt gatcaccaca gagacacaga gatcacagag gtttctccgt gtcctttgcg
    12901 cctctgtggt gaacggcttt tcactgccca accacgaaat cagaccgcac aaacccgatc
    12961 tgatcaccga agcgaacgag taccttcgcc gcgtagtgac catccggcaa ctcgaagttc
    13021 tccgagaggc gcacgggctt ggcggggtcg acgcgcgctg cctcgatcgt gatccgcctc
    13081 tctttgaagg ccacgaccgc gttgcctgaa tagacgtaga ccagcgcctc ggcttcgaca
    13141 tcctgcgaat cgctcagtgc cagcagctcg cgcgtggcaa tgtcgacgtc cacgacggcg
    13201 tgctttcccg acggagtcac agcggcgcga agcgtgaggc ccgtctgtgg caggtcattc
    13261 atcaggacgt cggcgaggtg caggccgtca ttcgacgcgg gtagcgagat ctcgttcgag
    13321 tacgaaggcc gatacgtaac gcgcgcgccg gaaggagcgt tgcgcacctt gacgcggatg
    13381 ccgttcgttt tccgttcgct ggtggggcgg aatcccagga cgtagacgac gcgctgcttg
    13441 tcggtcagct cctggatggc tacgttcatg tcgctcgtat gctcgaccaa tgtgccgccg
    13501 gtgtcgtgag tgagggcgaa gagcccctcg gtatcgatcg ctctctgtcg cggccgcaga
    13561 ccttcgatgg cgatggcatc gaggaagaca gaggctgccg tgaagtggcc aaacatctct
    13621 tgcatgtagg cgcccaggcg gggatcaaag cccaactgcg gattgcccgt caccgccaca
    13681 tcgttgtaac gcacgcgact cattgaattt tcataaggcg tggcgaacgc gttggttcct
    13741 ccgtgaagca tggagaacgt tccgtatccg gtcggaccga acgtcccgta tccggtgtgc
    13801 gttgtcggat gaacggactg gaccccgtga atgagcgaag catcgaagcc gccggaaagg
    13861 agcacaacgt gcttctggcc atcaagggct gccatgcggt cggccacggc gctaagcgcg
    13921 cggatctcgt cttcgactgc gtagcggagc ggctgtatgc tgagctcgcg cctgaatccg
    13981 acatcctgca ttcccggcgt gtccaggccg gccatggaac cgccgcctgt cgtcacggtc
    14041 aggaacagcg gatcgcccgc ttgcgcgctg ctcaggccac gcaatgcacg acgcagaaga
    14101 acctggtcgc gcgtaaaggg aacgacgaac tgaagaccgt ggttccccgt gtacgtcgcc
    14161 acgccaaaga catcgcccgg cagcgcgtgg tccacgtact gcagcgccgc tttttgcgcg
    14221 cgctgcaatg cggcgcgaga ggagaagcgc tcatcgaagg tgagcagata gagccgccgc
    14281 tgccgcggat cgctcgtcgt cgaagcgggc aacgccgcga agtcgatcgt gtcgaagtaa
    14341 tcgactggct gcggcttgcc gttgacgtag atctcgaagt catctttcgt caggcccgtg
    14401 acggcatggc cattcacgga gatgtacacc ggaacctgca cgacctcgac ggttgtaact
    14461 tcgtggtgaa ctgcgaaagc ctgcgatgag acgagaagga gcgccgaggc aaggagggtg
    14521 aggcgacggt tcatgtcatt ctccgatcgt tgtgttgcgt gttgatgaag ccgaggatga
    14581 atgcaagtgt ccgcgcgtcg gacgggatga tgctgaggaa cccgatggcc tgcctgcgtc
    14641 caccgtacca agtacagctc gtgtgatttc aggcacttag ccgcatccga tccatcgtgt
    14701 gtcgcctcca acggccacca gcgtcctcat tagtggttaa ggtgtagctc caatcccgat
    14761 cactgagcag cgtgatcccc g
//
LOCUS       DATLVW010000747         4016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_201352, whole genome shotgun sequence.
ACCESSION   DATLVW010000747 DATLVW010000000
VERSION     DATLVW010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201352"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VNN25_21820"
     CDS             <1..450
                     /locus_tag="VNN25_21820"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXH94230.1"
                     /translation="VVIISSLGALGGVILAGPRVYFAMARDGLLFRWIGVVHPRFQTP
                     HIAIVLQAVWASALVATGSYRSLFTRVVYTEWIFFALMAAGLFILRRRAAYAPTYRTW
                     GYPIVPIIFIAASAVIVGVQIAAEPMESVTGLLLVMAGLPVYYFWSR"
     gene            505..2013
                     /locus_tag="VNN25_21825"
     CDS             505..2013
                     /locus_tag="VNN25_21825"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94231.1"
                     /translation="MKRVLFTASTVVVALLLALVVTEGLLRLLNLPHEQKQPAKCKPY
                     VDGMTQAHSTSVVGAYAPGSSYTFCTSDYQVRYTADSLGYLGVLQKDPEHRPLVVFGD
                     SFAYGLGVEPEKSFAHLLDGYNAGLWGMSFDTHVLAFRRVIDEVNPTQAIWVLYPPHL
                     ITISDGSWMSRRVVDKKKHPLLFRAVAWFNSTKLSALLITTTGVGWNRSNYETLEYSL
                     YDAKDQSLETAYSSYEAAVVQVTAMARSRGVQLIPVFVPSRTEWALAEGHRPPLVHFG
                     HHFDSDLAVNRMSRILEDHGVPATQQIRLRPLIAKAVPRWEDLYFKSDAHWNASGHAI
                     VAQIIAAQLRRIQANSGLSPSGTSAAGRSGTGARSLADLSESWVSPPYAASNFSAGGS
                     MTWTVDRTDVTTYEYFLTGKAMVVAFNLSFTSVGGTPDQLLRIAIPGGFVPYRTVQNL
                     MMYNNNGGADSWGRVLAVKGQPTLWLFRPDNAPWSASANKTNVTGEITFEVQ"
     gene            complement(2073..2261)
                     /locus_tag="VNN25_21830"
     CDS             complement(2073..2261)
                     /locus_tag="VNN25_21830"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HXH94232.1"
                     /translation="MTKDFQQIAHETPEGNLLVEWWRFVRQNRKWWLLPIIIVVLCIS
                     LMVWLASGAAAPLIYSLF"
     gene            complement(2273..2689)
                     /locus_tag="VNN25_21835"
     CDS             complement(2273..2689)
                     /locus_tag="VNN25_21835"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HXH94233.1"
                     /translation="MNWDDLDLRPSARKLRDFGLVLLVFAVGAAIWQGVVRDRHLVML
                     LIGSFAFLSGIAALLRPALLLPLYRALTVATFPLGWVTSRLILAIAFYMVFCPIGLVM
                     RLIGRDRMRRRFDREAGTYWEPKTVPDDPASYLRQF"
     gene            complement(2699..>4016)
                     /locus_tag="VNN25_21840"
     CDS             complement(2699..>4016)
                     /locus_tag="VNN25_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXH94234.1"
                     /translation="LGLLYSAFTYYCGFKVNSGEYKLMGLAPYGKPLYADRILEKLLD
                     LKADGSFRMDMSYFNYGSGLTMTNGRFDALFGGPPRMPESALLPRHFDLAASIQVVTE
                     EILRRMAGEAHRQTGSNNLVMAGGVALNCVANARVLMDTPFANIWIQPAAGDAGGALG
                     AALFVWYQLLDHPRHAGAHDAQKGSLLGPSFEDDAIRNVVEGSGIAARHIEDEEELLE
                     IVSALLADERVIGWFQGRMEFGPRALGSRSILADARSPAMQAKLNLKIKFRESFRPFA
                     PIILAEEAGRWFDLPKCAESPYMLLTAPVANAHRRVLTPEETATMESDPDLRHRVNIA
                     RSTIPAATHVDYSARLQTIDQHRNPRLHRLLEAFLGRTGCPVLVNTSFNVRGEPIVCT
                     PFDALQCFLATGIDALVLGNFILEKSASQAMTAAEQETHLAQFQRD"
BASE COUNT          811 a         1194 c         1173 g          838 t
ORIGIN      
        1 gtcgtcatca tctcctccct aggcgcgctc ggcggcgtga tccttgccgg cccgcgcgtc
       61 tacttcgcca tggcccgcga cgggctgctc ttccgctgga tcggcgtcgt ccatccgcgt
      121 ttccagacgc cgcatatcgc catcgttctg caggctgtct gggcgtcggc gctcgtggcc
      181 acgggaagct accgaagtct cttcacgcgc gtcgtgtaca ccgagtggat cttctttgcg
      241 ctgatggcgg cggggctatt cattcttcgg agaagagctg cgtacgcgcc cacatacagg
      301 acatggggct atcccatcgt gccgatcatc ttcatcgccg cctcggcggt catcgtcggc
      361 gttcagatcg ccgccgagcc gatggagagc gtgaccggac ttttgcttgt catggccgga
      421 ctgcctgtgt actacttctg gtccagatga tcatcgactt tcttatgcct ctcagcgcgg
      481 ttcgaccgaa ggtatgattt ccgtatgaag agagtgcttt ttacagcctc gacggtcgtc
      541 gttgccttgc ttctggctct cgtcgtcacc gaggggctgc tgcgtcttct gaacttgcct
      601 cacgagcaga aacagcctgc gaaatgtaaa ccgtatgtcg acggaatgac ccaggcgcat
      661 tcaacctcgg tagttggagc gtacgctcca ggctcctctt acacgttctg cacgtctgac
      721 tatcaagtcc ggtacaccgc cgactcactg ggataccttg gagtccttca gaaggatccc
      781 gagcacaggc cgctcgttgt ctttggagac agcttcgctt acggattggg agtcgagccg
      841 gagaagagct ttgcacacct tctcgatggg tacaacgccg gcctgtgggg catgtcgttt
      901 gacacgcatg tcctggcatt cagacgtgtc attgacgaag tcaatccgac gcaggcgatc
      961 tgggtcttgt atccgccgca tctcatcacc atcagcgacg gctcgtggat gtcgcgccgc
     1021 gtggtcgaca aaaagaagca cccgttgctg tttcgtgctg tcgcgtggtt caactccacg
     1081 aagttgtcgg cgttgctgat caccacgacg ggcgttggct ggaaccgttc gaattacgag
     1141 acgctggaat actcgcttta tgacgccaaa gatcagtccc ttgaaactgc ctatagcagt
     1201 tatgaggcag ccgtagtgca agtgacggcc atggctcgaa gccgtggcgt ccagctcatt
     1261 cccgtatttg tgccatcccg gacggaatgg gcgctggccg aaggacaccg tccgcctctg
     1321 gttcacttcg gccaccactt cgattccgat ctggctgtca atcgcatgtc acgaatactc
     1381 gaagatcacg gcgtcccggc gacgcagcag atcagactca ggccattgat cgcaaaagct
     1441 gtcccgcggt gggaggatct ctatttcaaa tccgacgctc attggaatgc gagcggtcac
     1501 gccatcgtgg ctcagatcat cgccgcgcaa ctccggcgga ttcaagcaaa cagcggcctg
     1561 tcaccaagtg gcacgtccgc ggccggacgg tccggcactg gagcgcgctc acttgctgac
     1621 ctgagcgaga gctgggtctc gccaccctat gccgcgagca acttctccgc cggcggatcg
     1681 atgacgtgga ctgttgatag gacagacgtc acgacctacg aatactttct gaccgggaaa
     1741 gcgatggtcg tggcgtttaa tctgtccttc acctccgttg gcggaacccc tgaccaactg
     1801 cttcgcattg ccattcccgg aggcttcgtc ccttaccgga ccgtacagaa tctcatgatg
     1861 tacaacaaca atgggggcgc ggactcatgg ggtcgcgttt tagccgtcaa aggtcagcca
     1921 acgctctggt tattcaggcc tgataacgcc ccatggtccg cgtcggcgaa taagacaaac
     1981 gtaaccggcg aaatcacgtt tgaagtgcag tagccgggcg ttgcgagtaa cggagccagg
     2041 gactgttcga gaagccgagg ccgcaacgga attcagaaga gagagtagat gaggggagcg
     2101 gccgccccgc tcgccagcca gaccatcagg ctgatgcaca aaacgacgat gatgatcggc
     2161 agcaaccacc acttgcgatt ctggcgaacg aatctccacc actcaaccag gaggtttccc
     2221 tccggggtct cgtgcgcgat ctgctgaaag tcctttgtca tttcgttggt gctcagaatt
     2281 gtcttagata gctggccgga tcatcgggaa ccgtctttgg ctcccagtag gtgccggcct
     2341 ctcgatcgaa ccggcggcgc atgcggtcgc gcccgatcaa ccgcatgacc agtccgattg
     2401 ggcagaatac catgtagaac gcaattgcca gaatcagtcg tgaggtcacc caccccagcg
     2461 gaaacgtggc tacggtcagg gcgcgataca gcggtaacaa cagcgcaggc cggagcagag
     2521 cggcgatgcc ggacaggaac gcgaaggatc cgatcagcag catgaccagg tgacggtcac
     2581 gcacgacgcc ttgccagatc gctgcgccaa cagcgaagac cagaagcacg agaccaaagt
     2641 cccggagttt cctggcgctt ggtcgtagat cgagatcatc ccagttcatc agatcctctc
     2701 agtcgcgctg aaattgcgcg aggtgcgttt cctgctccgc tgcggtcata gcctgcgatg
     2761 cgctcttctc gagaataaag tttcccaaca cgagagcatc gatacctgtg gctagaaagc
     2821 attgcagtgc gtcgaaagga gtgcatacga tcggttcgcc acgcacgttg aagctggtat
     2881 tgacgagaac cgggcagccc gttctcccga gaaaagcctc gagcagccgg tgcagccgcg
     2941 ggttgcgatg ctgatcgatg gtctgcaagc gggccgagta gtcgacatgt gtcgcagcgg
     3001 gaatcgtcga tcgggcgatg ttgacacggt gcctgagatc ggggtcgctt tccatcgtcg
     3061 ctgtttcctc cggagtaagg acgcggcggt gtgcattggc gacgggcgcg gtgagcagca
     3121 tgtaggggct ctcggcgcat ttcggaaggt cgaaccagcg gccagcctct tcagcgagaa
     3181 tgatcggcgc gaaggggcgg aaactctcgc ggaacttgat tttcaggttc aacttcgcct
     3241 gcatggcagg cgagcgcgcg tctgccagaa tgctccttga gcccagtgcg cgaggtccaa
     3301 actccatgcg cccctggaac caaccgatca cgcgctcatc ggcgagtagc gccgagacga
     3361 tttcgagaag ttcttcctcg tcctcaatat gccgcgctgc gattccactg ccttcaacaa
     3421 cgttgcggat cgcatcgtct tcaaaagaag gcccaagaag gcttcctttt tgcgcatcat
     3481 gagcgccggc atggcgtggg tggtcaagaa gctggtacca gacgaacagg gctgccccga
     3541 gagcgccgcc ggcatccccc gccgccggct gaatccagat gtttgcaaac ggcgtatcca
     3601 tcagcaccct ggcgttggcc acacagttca gcgcgacgcc gccggccatg acgagattgt
     3661 tcgaacctgt ctgccgatgc gcttcaccgg ccattcgtcg caggatctct tctgtcacca
     3721 cttggatgct cgcagccaga tcgaaatgcc tcggcagaag cgcgctctcc ggcatgcgcg
     3781 gcggtccgcc gaaaagagca tcgaaacggc cgtttgtcat tgtgagtcct gacccgtagt
     3841 tgaaatacga catatccatc cggaagctgc cgtcggcctt caggtcgagc agcttctcga
     3901 ggatccgatc ggcataaagc ggcttcccat agggtgccag gcccattaac ttgtattcgc
     3961 cgctattcac cttgaatccg cagtagtaag tgaatgcgga gtagagcagt cctagt
//
LOCUS       DATLVW010000748         7615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_280686, whole genome shotgun sequence.
ACCESSION   DATLVW010000748 DATLVW010000000
VERSION     DATLVW010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280686"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VNN25_21845"
     CDS             complement(<1..1446)
                     /locus_tag="VNN25_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94235.1"
                     /translation="MVATAIAIPPVILARKRLWQASYVPPDTHRRFAFPHPRLKDKLA
                     FSAPGGEVLADRADDGRMMIPAIISTIQRDRVGDEVVPEGCRLDAYRRNPVVFFAHQD
                     LPIPVGLAETPNGELAVRIVAGKHVEADCYFHQSSKEGPQFYVLVRDGIIRSTSIGFN
                     PTEEPEKLGEIEGNLYAGYRFTGWELLEFSWVGVPANPYCGRVRQLLSAGKIAGEPIS
                     PMLRKSLEPLSEAAPIWEPSGFVQTVKSGDGREAEAQRAAFEQQFEHHAAHHPPARSI
                     LPGRHADRVLNGRLSLHLQRSAPETIRWNRSLSKAFDVEVMPSRACTAEYDWVSKHIG
                     CKVKHLYQNGTGVPSARMGSFLTGLKHVLSEHKLNDVRNLTYDGTERPPRYEVVQLNS
                     EKRDDFLVDGLAFYTAAHCKFALAVSPTWGGLILTVYTDQKDKGFNQQVLDRAWAWAK
                     TNNFLKGEAFALSGEFLNRTSESWDDVFLEEE"
     gene            complement(1641..1713)
                     /locus_tag="VNN25_21850"
     tRNA            complement(1641..1713)
                     /locus_tag="VNN25_21850"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1678..1680),aa:Met,seq:cat)
     gene            complement(1757..2023)
                     /locus_tag="VNN25_21855"
     CDS             complement(1757..2023)
                     /locus_tag="VNN25_21855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94236.1"
                     /translation="MLRIQSLIGECVAKPGVAVDDWFKNASLRRTVLQDPDVPRYCAG
                     WLGIDKRSVSIRWYGPTLGFGKTPEEAMLDCLKKADLDKPVLRC"
     gene            complement(2029..3303)
                     /locus_tag="VNN25_21860"
     CDS             complement(2029..3303)
                     /locus_tag="VNN25_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH94237.1"
                     /translation="MSPREERGLIIAATCRLNRQDDGTWLVPSQTNRETVAYRVNLEA
                     KTCTCPDHVEGGFTCKHYHAASIVHQRDVLPDGTVIETKSITLTQKTVYKQDWPAYNV
                     AQATEKRRVRVLLNELCRNLPDRERDSSKPGPKPHLTRDVVFAMAYKVYCGLSSRRFS
                     TDLLEAHEKGFVTKPIPGMKVAHFFEDPYFTPILKELIAQSALPLRSVEREFAIDSSG
                     FGSTRYEKWYDQKYGVTRTKCVWVKTHIACGVKTNVVTAVRILDKDAGDSPQFVPLVK
                     ETRKGFEIDEVSADKAYASLENFEEVAGCGGQGFIAFKSNHTGGVGGMFEKAFHYFQF
                     NQEEYMAHYHKRSNVESTFSAIKRKFGDSVVSKTDTAMTNEVLCKILCHNLTCLIQEQ
                     ETLGITPVFWKDEGQQEGDGPAVLPMLPMKRI"
     gene            3449..3862
                     /locus_tag="VNN25_21865"
     CDS             3449..3862
                     /locus_tag="VNN25_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94238.1"
                     /translation="MGIVVVSRGFAMSHEQVFVKVNAQVDSEIAPLVEALNAISEVVT
                     VSSCQGDNSRDAYVAFVMGNEWQELGDFVRRLSTALGQDKHLDDISYSLSVEWYAGGD
                     TPTGYLRVPHQHIQKLTEAVQRTSVNGLHHDDPAP"
     gene            complement(4556..5119)
                     /locus_tag="VNN25_21870"
     CDS             complement(4556..5119)
                     /locus_tag="VNN25_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94239.1"
                     /translation="MEQKNPLVILTTWIVNYEQHEEQLQTQLVAARTHVAMLEGNLAT
                     TQRWLAKFRDLLAILESTNNESIAILERDADATFKVAGTHFERIEAFLLAKKNEPRTV
                     AEIEAGTNIARSSISAVLYRTHSELFQAKFISGQRGKVWSLRNPAASMPPPPDPSLDP
                     PPDPVLDLVGMDEPDWVPDPEVEDIPF"
     gene            5507..6094
                     /locus_tag="VNN25_21875"
     CDS             5507..6094
                     /locus_tag="VNN25_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH94240.1"
                     /translation="MAKDATDQAMLKARKLFEKSGKSLDELGQAMGYEGDVARKAAWQ
                     FLNKTADPRLSMLRRFAKAMDVAPEELIATKKPKRGQSEKASREGQSMKARTAEEAEK
                     SHIIESRIQCRYETLTGGHLLKDDRLDRVRELPVRYLERLADELDRAIPQAKPRPGWP
                     SIIVTTVLAEYAHACRDEENEKLNASGCGPAEMTM"
     gene            complement(6368..6769)
                     /locus_tag="VNN25_21880"
     CDS             complement(6368..6769)
                     /locus_tag="VNN25_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94241.1"
                     /translation="MAEHCWHYVGSDEMFPSWGELHRQTCCRCGENRVIERRIVHGRR
                     VFHGPHWQPVLRIEEKELPWYNGMPSAECEGPDPPEGPINTEAIPTGFTIAEAEALIR
                     RLAAAYNGEDDVHEIGRLADEWVKRNPEPKP"
     gene            complement(6772..6939)
                     /locus_tag="VNN25_21885"
     CDS             complement(6772..6939)
                     /locus_tag="VNN25_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94242.1"
                     /translation="MANCNCDHKRNCPGWLSILAIWLVLWGHCDSIKTLEKAEKARAE
                     AEAKTGATHRE"
     gene            complement(6949..7158)
                     /locus_tag="VNN25_21890"
     CDS             complement(6949..7158)
                     /locus_tag="VNN25_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94243.1"
                     /translation="MADEQQPTLFGLPVHFTEKLPPMGGIQFVPADPDAYVVLSIECK
                     TCGWGGTFLAENAPKVCPRCVAELN"
     gene            complement(7284..7526)
                     /locus_tag="VNN25_21895"
     CDS             complement(7284..7526)
                     /locus_tag="VNN25_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94244.1"
                     /translation="MNPTPEQRALVAAEIREAATVAWLEAIRSNGTQELATPTKQAIF
                     ETGFLKGVTWALKASRISDVPKLRQDGDKNDLPSGD"
BASE COUNT         1498 a         2293 c         2233 g         1591 t
ORIGIN      
        1 ctcctcttca aggaagacat cgtcccagct ctcgctggtg cgattcagga actcgccgga
       61 cagagcgaag gcctcgccct tgaggaagtt attcgtcttc gcccacgccc aggcgcggtc
      121 gaggacctgc tgattgaacc ccttgtcctt ctggtccgtg tagaccgtga ggatcaggcc
      181 gccccacgtg ggagaaaccg ccagggcgaa cttgcaatgc gcggcggtgt agaacgctag
      241 gccgtcgacc aggaagtcgt ctcgcttctc cgaattgagc tgcacgacct cgtagcgcgg
      301 cgggcgctcg gtgccatcgt acgtgaggtt gcgtacgtca ttcagcttgt gctcgctgag
      361 cacatgcttg aggccggtca agaacgatcc catgcgggca gacggcacgc ctgtcccgtt
      421 ctggtaaagg tgcttgacct tgcagccgat atgcttggag acccaatcgt actccgccgt
      481 gcaggcccgg ctcggcatga cctcgacgtc gaacgccttg gacagcgagc gattccagcg
      541 gattgtctcg ggcgccgagc gctgcagatg gagcgacaac cggccgttca gcacgcgatc
      601 cgcgtgccgc cctggcagga tggaccgggc cggcggatgg tgagcagcgt ggtgttcaaa
      661 ctgctgctcg aaggccgcac gctgagcttc cgcctcgcga ccgtcgccgc tcttgaccgt
      721 ctgcacaaag ccgctcggct cccagatcgg cgccgcctcg gagagcggct ccagcgactt
      781 gcggagcatg gggctaatgg gttcgcccgc gatcttgccc gcggacagga gctggcggac
      841 gcggccgcag tagggattcg cgggcacgcc aacccacgaa aattccagca attcccaccc
      901 ggtgaaccgg tagccagcgt agaggttgcc ctcgatttcg ccgagttttt ccggctcttc
      961 cgtcgggtta aaaccgatcg acgtgctgcg aatgatgcca tcgcgcacga gcacgtagaa
     1021 ctgcgggcct tccttgctcg actggtggaa gtagcagtca gcctcgacgt gcttgccagc
     1081 gacgatgcgc acggccagct cgccgttcgg cgtctcggcg agtccgaccg ggatcggcag
     1141 gtcctggtgc gcgaagaaga ccaccggatt ccggcgatag gcgtccagcc ggcaaccttc
     1201 cggcacaacc tcgtcaccga cgcggtcccg ctggatcgtg ctgatgatcg cggggatcat
     1261 catgcggcca tcgtcggctc gatcagcgag gacctcgccg cctggggcag aaaaggccag
     1321 cttgtccttg agtcgcggat gcgggaacgc gaaccgccga tgcgtgtccg gcgggacgta
     1381 gctcgcctgc cagagccgct tgcgtgccag gatgacaggg ggaatcgcga tggccgttgc
     1441 aaccatgacg cgagcgtagt cacgtcagcc ggaaggcgtc aaagttgcgc ggggagcgag
     1501 cggaaaagaa agagcccggc attcgccggg ctcatgggcc gttcatcgca tggtggtcaa
     1561 gaggctggat cgtggttttg cggccgatct tttgggcgag tcgaacacac gcgctacagg
     1621 ttgttttgca acaccaggag taccgggggc aagattcgca cttgcggtct ccgagttatg
     1681 ggcccggcga gatggctgct tctccacccc ggcatagcag agagaatatc gcgacaggac
     1741 gtggtcgtca accctttcaa cagcggagaa cgggcttgtc gaggtcggct ttcttcaaac
     1801 aatcgagcat cgcctcttca ggcgtcttgc cgaagccgag cgtaggtccg taccagcgaa
     1861 tgctgacgct gcgtttgtca atgccgagcc agccagcgca gtaccgcggc acatcgggat
     1921 cttgcagaac tgtccgccgc agcgacgcat ttttaaacca gtcatccacc gcgacgcccg
     1981 gctttgctac gcattcacct ataagcgact gaatgcgtag caattgtatc aaatgcgttt
     2041 catcggaagc atcggcagaa cggccgggcc gtcgccttcc tgttggccct catccttcca
     2101 gaaaaccggc gtgatgccaa gcgtctcttg ctcctgaatc aggcacgtca ggttgtggca
     2161 cagaatcttg cagagcactt cgttcgtcat tgccgtgtcg gtcttgctca cgaccgaatc
     2221 gccaaacttc cgcttgatgg ccgagaaggt cgattcgacg ttgcttcgct tgtggtagtg
     2281 ggccatgtac tcttcctggt tgaactggaa gtagtggaac gccttctcga acatcccacc
     2341 gacgccgccc gtgtgattcg acttgaaggc gatgaatccc tgcccaccgc atccggccac
     2401 ttcctcgaag ttctccagcg aagcatatgc cttgtccgcg ctcacttcgt cgatctcgaa
     2461 gcccttgcgg gtttccttga cgagcgggac gaactgtggg ctatcgccag cgtccttgtc
     2521 caggatgcga accgccgtga cgacgtttgt cttgacgccg caagcaatgt gcgtcttgac
     2581 ccacacgcac ttcgtccgcg tgacgccata cttctggtcg taccacttct cgtaccgcgt
     2641 gctcccgaag ccgctggagt cgatggcgaa ctctcgttca acagaccgca aaggcagcgc
     2701 gctctgagcg atcaactctt tcaggatcgg cgtgaagtac gggtcttcaa agaagtgagc
     2761 caccttcatg ccaggaatcg gcttcgtgac gaagcccttc tcgtgagcct ccagcaggtc
     2821 ggttgagaac cgccgcgaac tgagaccgca atagacctta taggccatcg cgaacaccac
     2881 gtcgcgggtc aagtgcggct tcgggccggg cttgctggaa tcgcgttccc ggtccggaag
     2941 gttgcggcaa agttcgttca ggagaacgcg aacccggcgc ttctccgtcg cctgcgcgac
     3001 gttatacgcg ggccagtctt gcttgtagac cgtcttctgc gtcagggtga tgctcttcgt
     3061 ctcaatgacc gtgccgtccg ggagaacgtc ccgctggtga acgatggacg ccgcatggta
     3121 gtgcttgcag gtgaacccgc cttcaacatg gtccgggcaa gtgcaagtct tcgcttccag
     3181 gttgacccga tacgcgaccg tttcccggtt cgtctggctc ggcacaagcc aggtgccgtc
     3241 gtcttgccga ttgaggcgac aggttgctgc gatgatcagg ccacgttctt cgcgaggact
     3301 catgactact tctccctttg tgtctataac agtatagaca tacgggagga gaatgtcaat
     3361 atcacaatag acattctttc catcttctgg acttgcctac atcggtccgc tatgctaact
     3421 actggcgcag catttgccac aatgtcaaat gggtattgtg gtcgtaagca gaggcttcgc
     3481 catgtcgcac gagcaggtat tcgtcaaggt caacgcccag gttgattctg agattgctcc
     3541 tctagttgaa gctctgaacg ccatatccga ggtcgtcacg gtcagcagtt gccaaggcga
     3601 caatagccgc gacgcctacg ttgcgttcgt tatgggcaac gagtggcaag agttgggcga
     3661 ttttgttcgg cgattgtcga cggcgctcgg ccaagacaag catttagatg acatttccta
     3721 ttccctaagt gttgaatggt atgctggtgg ggacacccct acgggttatc tgcgagtgcc
     3781 ccaccagcat atccagaaac taacagaggc cgttcaacgg acatctgtta atggacttca
     3841 tcacgacgac ccggcgccgt aggttcccgt gttctgatgg cacggaatgc agagcgcctc
     3901 acagttggac aggctgtcgt ccccgccagc gtcaacggca gtgcgatgat gcgcgtgcca
     3961 gttgcccggc gtgagcactg cattgcatct gccgcccggg tgcctgtgcg agatgcgagt
     4021 gcattcacac ctgccgccgg ccctgcggaa cgcccgacct ttcgtatctt ctgaaaatgc
     4081 catagtcgaa attcctctgg agcggttgac accggccggc ccgccacgta ctcttggcat
     4141 caccgtgatg ccacaggcga acttccggtc ggcacgggct ccaccgtgcc ggttgagaga
     4201 agaatcggcc tgatgcgaac ggaggaactt gcatccattc gggccaccgc ggtcaacggt
     4261 tgaggcccac cggcttccgt agtcggcgcg tctgctactt ccgccacgtc cgcatcgtgg
     4321 ttgttgcggt tcgtccgaaa aggacgagga aacccagccc ggcaggggcg cagcatagag
     4381 ctcgcccttg ccgggcttcc gtttgatttg acgtcggtgg ggcgcgagct aaaataaaga
     4441 tggagccaga ctgcgtgtgt ccgaacatgg cgataccttt tcggctagca gttcgggttc
     4501 ccacggccgg ggcgtgtacc agacgctgcc ggccgaaccc gtttactgac caaactcaaa
     4561 acggaatgtc ctcaacctct ggatctggca cccagtctgg ctcgtccatc cccaccagat
     4621 caagcaccgg gtcaggtggc ggatctagac taggatcggg aggtggaggc atactggcgg
     4681 ccgggtttcg cagagaccaa accttcccgc gttggccaga gatgaacttc gcctgaaaca
     4741 gttcgctgtg cgtcctgtac aatacggcag aaatcgaaga cctagcgata ttcgttccgg
     4801 cctcaatttc agcgacggtc cgtggctcgt tcttcttcgc aagcagaaat gcttcaatcc
     4861 gctcgaagtg tgttcccgca accttgaatg tagcgtcagc atcacgctca aggatggcga
     4921 tggactcgtt gttggttgac tcaagaatcg cgagtaggtc tctgaatttt gccaaccagc
     4981 gttgagtcgt ggccaggttg ccttctagca ttgccacgtg cgttctcgcc gctacaagtt
     5041 gcgtttgaag ttgctcttca tgctgctcat agttaacgat ccacgtggtc agaatgacga
     5101 gcgggttctt ttgctccatg acgcaccccg agctattgac ggttcctgga ccgtctgtaa
     5161 aatgaaaatg ccacgggcgg gaaggcgata gagccatccg cttgcggctt ccaacgcgat
     5221 cctgtctctt gatttagagc catgaggcag gatcgcaccg acttcaacaa gctctccata
     5281 agtcagctgc accagctgac ttatggagag ctttcctttt gtgtacctta ctgatctgct
     5341 cccgcttccg caagtcattt cagagaataa attcagagga cgattcgcgt tcgtgaaaca
     5401 tggccaaatg aataggtact cgatgcgact tgggcagatg cgtatttccg cgcgattcgc
     5461 cgctagcaac cttgatggaa cgtctatttc tcgatagact atcggcatgg cgaaagacgc
     5521 aaccgaccag gccatgctga aggcacggaa gctgttcgag aagtctggga agtccctgga
     5581 cgaactcggg caagcgatgg gttatgaagg agatgtcgca cgtaaggcgg cttggcaatt
     5641 cctcaacaag actgccgatc cccgactctc gatgctgcgc cggttcgcga aagcgatgga
     5701 tgtcgcaccg gaagaactaa tcgcaacgaa gaagccgaag cgaggtcagt ccgagaaagc
     5761 ttctcgtgag ggtcaatcaa tgaaagcgcg caccgcagaa gaggcggaga agtctcacat
     5821 catcgagagc cgcattcaat gtcggtatga gacgctaacg gggggacatc tcttgaagga
     5881 tgataggctt gatcgtgtac gagaactgcc ggtccgctat ttagaaaggc tcgccgatga
     5941 gcttgatcgg gccattccgc aagcgaagcc gcgacctgga tggccatcca taatcgtcac
     6001 gactgtcctt gccgagtacg ctcatgcgtg ccgcgacgaa gagaatgaga aactgaatgc
     6061 gtccggctgc gggccagccg aaatgaccat gtagtccatt gcctagccag ccgcgttgcg
     6121 cccaggacga ttgggtatca gcccgcccga atgcgtcgca ggtctcgaat gggtagcaaa
     6181 gccccaccat cgcccaggaa ccgaccaagc gtcgcagtct ctccgtcccg catgcggcta
     6241 agcactacgc tgtgggcata gccagcctct tccgctgcat ccgccaggac gggcaggtcg
     6301 caccatcgct gttgatccca gatcgtctga gcgatcaccc gaaccgtcgg cgtcatccaa
     6361 ggcaccgtca tggcttcggc tccggattac gcttcaccca ctcgtccgca agccggccga
     6421 tctcgtgaac gtcgtcctcg ccgttgtatg cggcggccag gcgacggatg agggcttccg
     6481 cctctgcgat cgtgaaaccc gtcggaatgg cctctgtgtt gattggtcct tccggcgggt
     6541 ccgggccttc gcactccgca gatggcattc cgttgtacca gggcaactcc ttctcctcga
     6601 tgcgtagcac gggttgccag tgcgggccgt ggaagacgcg gcggccgtgg acgatgcgac
     6661 gctcaatgac gcgattctca ccacagcgac agcatgtctg acgatgaagc tctccccagg
     6721 acgggaacat ctcgtccgag ccgacatagt gccagcagtg ctccgccatc ttcactcccg
     6781 gtgcgttgcc cctgtcttcg cctcagcttc tgcgcgtgct ttctcggcct tttcgagcgt
     6841 cttaatgctg tcgcagtggc cccagagcac aagccagatg gcgaggatgg agagccagcc
     6901 agggcagttg cgtttgtggt cgcagttgca gttcgccatc acgttacctc aattcaactc
     6961 cgcgacgcat cgcgggcaga ccttcggcgc gttctctgca agaaacgtcc cgccccatcc
     7021 gcacgttttg cactcgatgc tgagcacgac gtatgcgtca gggtccgccg gaacgaattg
     7081 gattccgccc atcggcggca acttctccgt gaagtggacc gggagaccga agagggtcgg
     7141 ctgctgctcg tcggccatcg aacatctcca tttcgacgcg ccgcaactcg gcgcgacaaa
     7201 actcctcgaa tataccgcag gccttccaga actctgcgtc cgccgctgac cactcaatgg
     7261 gctgaatggc gaggccgggc atgtcagtcg ccggaaggaa gatcgttctt atcgccgtct
     7321 tgacgcagtt ttggcacatc agaaatccga ctagctttga gcgcccaagt cacgcccttc
     7381 aagaacccgg tctcgaagat cgcttgcttc gtaggcgtcg caagctcctg agttccgttg
     7441 gatcgaatcg cctccagcca cgcgaccgtt gcagcttcgc gaatctccgc ggcgacaagg
     7501 gcgcgttgct cgggggttgg attcatgatg tcgctccctc acgatccgcc cgcgttcgtg
     7561 ccctgaatcg tacacgtcgc accgctcatt atcgcggccc actcgttcgc tgtca
//
LOCUS       DATLVW010000749         8532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_157599, whole genome shotgun sequence.
ACCESSION   DATLVW010000749 DATLVW010000000
VERSION     DATLVW010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157599"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VNN25_21900"
     CDS             complement(<1..1429)
                     /locus_tag="VNN25_21900"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HXH94245.1"
                     /translation="MRLAALPNLSKHVRQAFQIAAATAQANGESRIRGRHLFYGMLSV
                     KVCSVAKALATLKITPADVQLKDKTRITGYASDVSDGTDRLGIEREARVFAAVLGASK
                     VEPPIAVGLLGTWGSGKSFFMHELKRQFVNVESRGRSGDEESCSNIVQIWFNAWHYID
                     SNLWASLTSTIFDALDEELTIRAGALTDAESSADKKATLEADKARLQSEREAAGRDRS
                     AAEAELQAIADTRHDLEDDDEVARRAGVSRIGSAAVEAVGKNPEVQQIAAEATNAAAK
                     FAKDYGLDRETVESMRGTAGKLYGLFQVVRKKRGLWTWLLLGGIVLGSLALALIPQLK
                     QLIRYTGSISLVGAAGSVVWLLALVKKYLPAVNEVVKRVDAFFLAKDELVGKLRKDQL
                     KDLDMRGKEATERLSQAKAREQEAEKELRLKERDLMELRPMQQMKSFVRERRDSTDYT
                     QHLGVIAHAHEDFDRLSRHLATVRKQ"
     gene            1613..1975
                     /locus_tag="VNN25_21905"
     CDS             1613..1975
                     /locus_tag="VNN25_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94246.1"
                     /translation="MRERTPRRTVEEREKLFVRHFPRMECGGDESCWNHCPRIRAEDS
                     DELSFHDDRRTALRRRLCCVIELRNEFSTALGDPPLLNIDPFKCRPNADARPDDEFAE
                     RESGPNLAQLQRDEIQGW"
     gene            complement(2264..2566)
                     /locus_tag="VNN25_21910"
     CDS             complement(2264..2566)
                     /locus_tag="VNN25_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94247.1"
                     /translation="MLVPTPPDRMVDAQGRPYFLWDVDMTLDELTRQLAEADLPVRAY
                     LTGKVMRQAKPDDALQFISAQQMADLWPHLEKYLGKTREFWSWLLEEWKRRGKVRG"
     gene            2608..3456
                     /locus_tag="VNN25_21915"
     CDS             2608..3456
                     /locus_tag="VNN25_21915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXH94248.1"
                     /translation="MQEGQASRTADRVAERRAAHQVRDRPLVFEDPLALRIIRPEVAR
                     ELRERPPTHDSSPIAPYLRAFFAMRSRFAEDALREAVGRGVQQYVVLGAGFDTFAYRN
                     PYPALRVFEVDHPATQATKRERLVSGEITIPPSLTFVSIDFATMALDVALRNAGFDAA
                     APAFFAWLGVVPYLERPAIDQTLSFIAALPAGTAVVFDYSVPPESLSWMSRLVFHKMA
                     KRVAAAGEPWKTFFDPEELLRHLHELGFSNAEDFDGDALNRRFFSGRSDKLKVGAGGH
                     MAMARV"
     gene            complement(3540..4109)
                     /locus_tag="VNN25_21920"
     CDS             complement(3540..4109)
                     /locus_tag="VNN25_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH94249.1"
                     /translation="MKARSLAKATYEDLLKVPENLVAELIDGELYAWPRPAGPHARFS
                     SVLGMEIGPPYDRGRGGPGGWWILDEPELHLGENVLVPDLAGWRRERMPEIPHDHRFI
                     IEPDWVCEVISPSTVRVDRGKKVFIYARHDVPWLWFVDPEPRMLEVLQLAGDVYSLMH
                     VFTGDDVVRADPFPAAEIDLASIWGPTEE"
     gene            4155..5546
                     /locus_tag="VNN25_21925"
     CDS             4155..5546
                     /locus_tag="VNN25_21925"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXH94250.1"
                     /translation="MLRRLSFAFLILLTTISAAAAARKVDLIVTGGTVITMAGPNIDD
                     GAVAIDKGDIVAVGSSADIAKRFAGTDTIHARGSAILPGFVNAHTHVPMTLFRGIADD
                     RDLMDWLQHFIFPAEAKNVDRDFVRWGTRLAAAEMIASGTTTFTDMYYFESDIAAEAK
                     KAGLRAVVGETMIDFPVADNKTWDAAIAYVREFVTHWRGDRLITAALAPHSPYTVSRA
                     HLEQVRALATELHAPILIHVAETKNEAAQIKDNTSGLSPAAYLDSIGFLGHDVIAAHG
                     VWLTPDDIQTLAKKRVGVAHCPESNMMLASGVAHVVDMRKAGVSVGLGTDGPAGSNNN
                     LDMVEEAASAARLQKITLGDPKALSARDVLEMATIGGAKVLGMEDRIGTLEAGKRADV
                     IVIDLQQPKTQPVYAVESAIVYAASGNSVVTTICDGKVLMRNRKVLTVDVPAAVAKAK
                     EYRDRVRKSLASK"
     gene            5683..6117
                     /locus_tag="VNN25_21930"
     CDS             5683..6117
                     /locus_tag="VNN25_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HXH94251.1"
                     /translation="MYDERIAGPMRQELTQLGFQETRTPQEVDAVMQEKKGTVLVVVN
                     SVCGCAAGVARPAIALSLENEVKPDKMITVFAGNDRDATNRARDYFVGYRPSSPSIAL
                     FRDGELVKMVERHQIEGRAAHDIASELAAAFNQYCAKEVAVQ"
     gene            complement(6118..7617)
                     /locus_tag="VNN25_21935"
     CDS             complement(6118..7617)
                     /locus_tag="VNN25_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HXH94252.1"
                     /translation="MSTLGTKVRSIRRRERITQADLAAKLEISPSYLNLIEHNQRPLP
                     AHLLVKVAQVLKVELNSFADDDHSRLAGDLQEAFGDPMFEEHAVTTVDMRDLAEHPAT
                     ARAVLALYQAYRSTVESTRALASKVYDGQDFHGIDPAHLPSEEVSDVLQHNVNYFPEL
                     EAAAEEVGGRALPNRSDVNRSLTQYLREKHRIEVAVVPVGRDRTATRRYDPERRVLSL
                     SAVLPPWSRQFQLAHQLALIEAVPLLDSIVERSKDFLTTPESAKLCRIALASYFAAAL
                     LMPYDEFLSISEQVRYDIELLQHHYTASWEQVCHRLTTLRNPSRLGVPFHFVRVDIAG
                     NISKRFSGTGIRFARFSGSCPRWDVHAAFLTPGRIRTQLSKMPDGTAYFCVARTIRKS
                     FGYATGDALMAVGIGCPVSDARKLVYADGYDLDNLDAAVPIGTTCRLCERLDCEQRAF
                     PPLQHGFTIDENVRGASFYAPAEGVRASRPQALGRPRPSAKAESTKRKS"
     gene            7790..>8532
                     /locus_tag="VNN25_21940"
     CDS             7790..>8532
                     /locus_tag="VNN25_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HXH94253.1"
                     /translation="MTTAVIEVGAGIDVRGDVRPEWSEILTPEALAFVAGLHREFNPR
                     RLELLARRGARKQRIDAGELPGFLQETQEIRDREWTVAPIPADLQDRRVEITGPVDRK
                     MIINALNCGASVFMADFEDANAPSWANNIEGQLNLRDAVRRTISFTAGAKQYVLHEKT
                     ATLVVRPRGWHLPEKHLLVDGEPVSASLFDLGLYFFHNAKEAVARGTGPYFYLPKMES
                     HLEARLWNDVFVRAQKELGVPNGTIRATVL"
BASE COUNT         1591 a         2627 c         2702 g         1612 t
ORIGIN      
        1 cttgtttgcg aaccgtagcg aggtggcgac tgagccggtc gaagtcttcg tgcgcgtggg
       61 caatcacgcc gaggtgttgc gtgtagtcgg tgctgtcgcg tcgttcgcgg acgaagctct
      121 tcatttgttg catcggcctc agttccatga gatcgcgctc tttcaaccgc agctcttttt
      181 ccgcttcttg ctcgcgcgct ttggcctgag acaaacgttc ggtggcctcc ttgccgcgca
      241 tgtcgagatc cttgagttga tccttgcgca gttttccgac aagttcgtct ttcgcgagga
      301 agaacgcgtc gacgcgcttg acgacctcat tgacagccgg cagatatttc ttcaccagag
      361 ccaggagcca aacgacactt ccggcggctc cgaccaagct aatggagccg gtgtagcgga
      421 tgagctgttt gagttgcggg ataagcgcaa gggcaaggga gccgagaacg attcctccga
      481 gcaggagcca ggtccacagg cccctttttt tcctgacaac ttgaaagagc ccgtaaagct
      541 tgcccgccgt tccgcgcatc gactcgactg tctcccggtc aagcccgtaa tcctttgcaa
      601 acttggcggc agcattggtc gcctctgcgg cgatctgctg cacctctgga ttcttgccga
      661 cagcttctac cgcggcgctg ccgatacggg aaacgccggc gcgccgcgct acttcgtcgt
      721 catcttcgag gtcgtgtcgc gtatccgcga tcgcctgcaa ctccgcctcg gctgcgctgc
      781 ggtctcttcc agctgcttcg cgctccgact gcaggcgcgc tttgtcggcc tccaatgtag
      841 cctttttgtc cgcgctgctt tcggcatcgg taagagcgcc agcgcggata gtcagctcct
      901 cgtcgagggc gtcgaagatt gtcgaggtga gactggccca caggttcgag tcgatgtagt
      961 gccaagcgtt gaaccagatc tgaacgatgt tcgagcatga ctcctcgtcc ccagaacggc
     1021 cgcggctttc aacatttaca aattgccgct tcaactcgtg catgaaaaag ctcttcccgc
     1081 ttccccatgt acccagtagt ccgaccgcaa tcggaggttc gactttactc gctccgagca
     1141 cggcagcgaa tacacgtgcc tcccgctcaa tcccgaggcg gtcggtgcca tcggagacgt
     1201 ctgaggcgta cccggtgatt cgagttttgt ccttcagctg gacgtcggcg ggcgtaatct
     1261 tgagagtcgc gagagctttc gcgaccgagc agaccttcac agagagcatg ccgtaaaaaa
     1321 ggtgacggcc ccggattcgg gactcgccgt tggcttgtgc ggtggccgcg gcgatttgaa
     1381 acgcttggcg gacgtgcttt gaaaggtttg gaagcgccgc gagccgcatg agctggtagc
     1441 cgttttcgct gggcgaaata ctcttgaccg cgtcggtcag agcctttcga agagccggct
     1501 cgtcaatgcc gcgcgaagtc acaaggcggc gtgtgctgcc atcggggttc tcaaaaagcc
     1561 ccaggacgag atgctccgcg tgcaccgttc ctttgagtgc cataccgctc gcatgcgcga
     1621 gcgcacgccg cgccgaaccg tcgaggagcg agaaaagttg ttcgtcaggc atttcccgcg
     1681 gatggagtgc ggcggcgatg agtcttgctg gaatcactgt ccgcgcatcc gagccgagga
     1741 ctccgacgag ctttccttcc acgacgatcg gcgaaccgcc cttcggcggc gtctctgctg
     1801 cgtgatcgaa ttgcgcaatg aattcagcac cgctttggga gatccgccct tgctgaacat
     1861 cgatcccttc aaatgccggc caaatgctga cgcacggccc gacgacgagt tcgccgagag
     1921 ggagagcggc cctaacctcg ctcagcttca gcgcgacgag attcaaggct ggtaactcgt
     1981 cgtccataga tgcggtggaa cttctgaagg tatccgctgc aaacgcgacc ctgtagatcg
     2041 acgttggcga aaggactccg gctagagtca ccgcaaatgc gggttcgact agtgtggcaa
     2101 cggcgattag ttggtcgcct gaagggtccc agaccgagat cgctaccgag cgcactgctt
     2161 caggttgcgc gtccgccatt cgtcctccag atttgagaag gacttattgt agccctgcac
     2221 tggcttgaag acgagcaaat cgcgaggaat tcttggcccg agactacccc ctgacctttc
     2281 cgcgacgttt ccactcctcg agcagccaac tccagaactc gcgcgttttg ccgaggtatt
     2341 tttcgaggtg tggccacagg tcggccatct gttgcgcgga tatgaactgc agggcgtcgt
     2401 cgggcttcgc ctggcgcatg acctttccgg tcaggtaggc gcgaacgggg agatcggcct
     2461 ccgccaactg gcgcgtgagc tcgtcgagtg tcatgtcgac gtcccagaga aaataggggc
     2521 gcccctgcgc gtcgaccatt cgatcgggag gagttgggac gagcatgaga gaagcgtagc
     2581 agatcgaaga atccgtacaa tcggaacatg caggaggggc aggcaagccg gacggcggat
     2641 cgggttgcgg agcggcgcgc ggcgcatcag gtgcgtgacc ggcctctcgt cttcgaggat
     2701 ccgctagcgc tgcgcatcat ccgtcccgag gttgcgcggg agttgcggga gcgtccgcca
     2761 acgcacgatt cgtcgccgat tgcgccgtat ctgcgcgcct tcttcgccat gcgcagccgg
     2821 ttcgccgagg atgcgttgcg cgaggccgtc gggcgcggcg tgcagcagta cgtcgtcctc
     2881 ggcgcgggct tcgatacctt tgcttaccgc aatccgtatc cggcgctgcg cgtcttcgaa
     2941 gtcgatcatc cggccacgca ggcgacgaaa cgggagcggc tcgtgagtgg cgagatcacg
     3001 attcctccgt cgctcacgtt cgtgtcgatc gatttcgcaa cgatggcgct cgacgtggcc
     3061 cttcggaatg cgggcttcga tgcggcagcg ccggcgttct ttgcgtggct cggcgtggtg
     3121 ccgtatctcg aacggccggc gatcgatcag acgctctcgt tcatcgcggc attgccggcc
     3181 ggtactgcag tcgtcttcga ctacagcgtc ccgccggaat cgctcagttg gatgagccgg
     3241 ctcgtcttcc acaaaatggc caaacgcgtc gccgccgcag gagagccgtg gaagacgttc
     3301 ttcgatccgg aagagctatt gcgtcatctg cacgagctcg gattcagcaa cgccgaggac
     3361 ttcgacggcg atgcgctgaa ccgccgcttc ttttcgggcc gaagcgacaa gctgaaagtc
     3421 ggcgcgggtg ggcatatggc gatggctcga gtctagtcac caaagcctga accacagaga
     3481 cacagaggac acagaggaag gaatggccgt agcctcagtg ctctctgagt ctctgtggtt
     3541 cattcttccg tcggacccca gatcgaagcg agatcgatct cggcggcggg aaagggatct
     3601 gcgcggacga cgtcatcgcc agtaaacacg tgcatcagtg agtacacatc gccggctagt
     3661 tgcaacactt cgagcatccg cggttcggga tcgacaaacc aaagccacgg cacgtcatgc
     3721 ctggcgtaga tgaatacctt cttgccgcgg tcaacgcgaa cggtcgaagg cgagatcact
     3781 tcgcataccc aatccggctc gatgatgaaa cggtggtcat gcggaatctc cggcatgcgc
     3841 tcgcggcgcc aaccggcaag atcggggacg agcacgtttt cgccgaggtg aagctccggc
     3901 tcatcgagga tccaccagcc gccgggaccg ccgcgtccgc ggtcgtacgg tggtccgatc
     3961 tccattccaa gcaccgatga gaaacgggca tgcggtccgg cgggccttgg ccacgcgtac
     4021 agctccccat cgatcaactc ggccacgagg ttctcaggga ccttcagcag atcctcgtac
     4081 gtcgccttcg cgaggctcct cgctttcatg acgtcaattg tacgctcgcg ctctttgcgt
     4141 taacgtccgc ggacatgctc cgacgcctat cgtttgcttt cctcattctt ctgaccacga
     4201 tcagcgctgc cgcagcggcg cgcaaggtcg acctcatcgt caccggcggc accgtcatca
     4261 cgatggccgg cccgaacatc gacgacggcg ccgtcgccat cgacaaaggc gacatcgttg
     4321 cggtcggctc atccgctgac atcgccaaac gcttcgctgg aacggacacg atccacgcgc
     4381 gcggctcggc catccttccc ggcttcgtca acgcgcacac gcatgtgccg atgacgctct
     4441 tccgcgggat cgccgatgac cgcgatctca tggactggct gcagcacttc atctttcctg
     4501 ccgaggccaa gaacgtcgac cgcgatttcg tgagatgggg aacgcggctc gcggccgcgg
     4561 agatgatcgc gtcgggaacg accaccttca ccgacatgta ctacttcgag agcgacatcg
     4621 cagccgaggc gaagaaggcg gggctgaggg ctgtcgttgg cgagacgatg atcgacttcc
     4681 cggtcgccga caacaagacc tgggacgcag ccatcgcgta cgtccgggag ttcgtcacgc
     4741 actggcgcgg cgaccggctc attaccgccg ccctcgcgcc gcattcgccg tacaccgtat
     4801 cgcgcgcgca cctcgaacaa gtccgtgcgc tggcaacgga gctccacgcg ccgattctca
     4861 tccacgttgc agaaacaaag aatgaggccg cgcagatcaa ggacaacacc agcggcctct
     4921 cccccgctgc ctacctcgac tcgatcggct tcctcgggca cgacgtcatc gccgcgcatg
     4981 gcgtctggct cacgcctgat gacattcaaa cccttgcgaa gaaacgcgtc ggcgtggcgc
     5041 actgccccga gagcaacatg atgctcgcct ccggcgtcgc ccacgtagtc gacatgcgca
     5101 aagccggcgt ttccgtcggc ctcggcaccg acggtccagc cgggtcgaac aacaatctcg
     5161 acatggtcga agaagccgcc agcgccgcgc gtctgcagaa gatcacgctc ggcgatccga
     5221 aggccctcag cgcccgcgac gtcctcgaaa tggccaccat cggcggcgcc aaagtcctgg
     5281 gcatggaaga ccgcatcggt acgctcgaag ctggcaagcg cgccgacgtc atcgtcatcg
     5341 atctgcagca gccgaagacc cagccggtat acgccgtcga gtcggcaatc gtgtacgccg
     5401 cgtccggcaa ctccgtcgtc acgacgatct gcgacggaaa agtcctgatg cgcaaccgca
     5461 aggtattgac ggtcgatgtg ccggcagcgg tggcaaaggc aaaggagtat cgcgatcggg
     5521 tgaggaagag cctggcatcg aagtagacac gccgcatctg tcatccttat gaccgtgagg
     5581 gtgacacttc cgacaagtgt gttccgctca cacccacgcc ccactcccca cccccgaatt
     5641 ttcacggtac gatttcgctt ttcagacacc ggaggagaac caatgtacga cgaacggatt
     5701 gctggtccga tgcgccagga gctgacgcag ctcggatttc aggaaacacg cacgccgcag
     5761 gaagtggatg cggtgatgca ggagaagaag ggcacggtgc tcgtggtcgt gaactcggtg
     5821 tgcggatgcg ctgccggcgt ggcccgtccc gcgattgcgc tctcgctcga gaacgaggtg
     5881 aagcccgaca agatgatcac ggtcttcgcg ggcaatgacc gcgatgcgac gaaccgggcc
     5941 cgtgactact tcgtcggtta ccggccatcg tcgccgtcga tcgcgttgtt ccgcgacggc
     6001 gagctggtga agatggtgga gcggcatcag atcgagggac gcgcggcgca cgacatcgcg
     6061 tcggagctgg cggcggcgtt caaccagtac tgcgcgaagg aagtggcggt tcagtagtta
     6121 cgattttcgt tttgtcgatt ctgctttcgc actcgggcgg ggacgcccga gtgcctgcgg
     6181 gcgagacgcc cgcactcctt cggcgggggc gtagaaggag gcgccgcgga cgttttcgtc
     6241 gatcgtgaag ccgtgctgca ggggcgggaa ggcgcgctgc tcgcagtcga ggcgttcgca
     6301 gagacggcag gtcgttccga tcgggacggc cgcatcgagg ttatcgagat cgtagccgtc
     6361 ggcgtagacg agtttgcgcg cgtcgctgac cgggcagccg atgccgaccg ccatcaatgc
     6421 atcgccggtc gcgtagccga aactcttgcg gatggtccgc gcgacgcaaa agtacgcggt
     6481 gccgtcgggc atcttcgaga gctgcgtccg gatccttccc ggcgtcagga atgccgcgtg
     6541 aacatcccat cgcgggcacg agcctgagaa gcgcgcgaag cggatgccgg tgccgctgaa
     6601 gcgcttggag atgttgccgg cgatgtcgac gcggacgaag tgaaagggga cgccgagccg
     6661 ggaagggttg cggagagtgg tgaggcggtg gcagacctgc tcccagctcg ccgtgtagtg
     6721 atgctggagc agctcgatgt cgtagcgaac ctgctcgctg atcgacagga actcgtcgta
     6781 cggcatcagc agcgcggcgg cgaagtagct ggccagcgcg atgcggcaga gcttcgccga
     6841 ctccggcgtc gtcagaaagt ctttggagcg ctcgacgatg gaatcgagca gcggcaccgc
     6901 ttcgatgagc gccagttggt gcgcgagttg gaactgccgg ctccacggcg ggagcaccgc
     6961 ggagagcgag agcacacgcc gctccggatc gtagcgccgc gtggccgtgc ggtcgcggcc
     7021 gaccggtacg accgccactt cgatgcgatg tttctcgcgc aggtactgcg tcagcgagcg
     7081 attaacgtcg gagcgattcg gcagcgcgcg tccgccaact tcctccgccg ctgcttcgag
     7141 ctcggggaag tagttgacgt tgtgctgcag aacatccgac acctcctccg agggaaggtg
     7201 ggccggatcg atgccgtgaa agtcctggcc gtcgtagacc ttgctggcca gtgcgcgcgt
     7261 cgactccacc gtggagcggt aggcctgata cagcgccagc accgcccggg cggtggcggg
     7321 atgttcggca agatcgcgca tgtcgacggt ggtgacggcg tgctcttcga acatcggatc
     7381 gccgaatgct tcctgaagat cgccggccag acgggagtga tcgtcgtcgg cgaacgagtt
     7441 gagctcgacc ttcagcacct gcgcgacctt cacgagaaga tgcgcgggaa gcggccgctg
     7501 attgtgctcg atgaggttga gatagctcgg cgagatctcg agcttcgccg cgaggtccgc
     7561 ctgcgtgatc cgttcgcgac ggcggatgga gcggactttg gtgccgaggg tgctcatgga
     7621 gatctcctga actggcacgg tggttggact gtcacgagtg ctgcctgaac gacgctcacg
     7681 tttctttaca accgttgaca actttacggc ggtgacttca caactcttta ctgcgtaaca
     7741 tacagatggg tatcaacttg gcccattccg tgctcaacta ctttacacca tgacgacggc
     7801 agtgatcgaa gttggagcgg gaatcgatgt tcgcggcgac gtgcgccccg aatggtcgga
     7861 gatcctgacg ccggaagcac tcgcattcgt ggccggactg catcgcgagt tcaatccgcg
     7921 gcgcctcgag ctcctggcgc ggcgcggagc gcggaagcag cgcatcgacg cgggcgagct
     7981 tcccggcttt ctgcaggaga cgcaagagat tcgcgatcgc gaatggaccg tcgctccgat
     8041 tcccgcggac ttgcaggatc gccgtgtcga gatcaccggg ccagtcgacc ggaagatgat
     8101 catcaacgcc ctcaactgcg gcgcgagcgt gttcatggcc gacttcgaag acgccaacgc
     8161 gccgtcgtgg gcgaacaaca ttgaaggaca gctcaacctt cgcgatgcgg tgcggcgcac
     8221 gatctcgttc acggccggag cgaagcaata cgtgctccac gaaaagaccg ccacgctcgt
     8281 ggtgcggccg cgcggctggc acttacccga gaagcacctc ctcgtcgatg gcgagccggt
     8341 gtcggcaagc ctcttcgact tgggcctcta cttctttcac aacgcgaagg aagcggtcgc
     8401 gcgcggaacc ggtccgtact tttacctgcc gaagatggag tcgcacctcg aggcgcggct
     8461 ctggaacgat gtgttcgtcc gcgcgcagaa agagctcggc gttccgaacg gaacgatccg
     8521 cgccaccgtc ct
//
LOCUS       DATLVW010000750         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_75588, whole genome shotgun sequence.
ACCESSION   DATLVW010000750 DATLVW010000000
VERSION     DATLVW010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75588"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VNN25_21945"
     CDS             <1..1251
                     /locus_tag="VNN25_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94254.1"
                     /translation="PQHKNTAMYLQDHWTLKNVTLNAGVRWDRQRIIDASGVTQIDLK
                     KDLAPRLGLIWDPSGTNQTKVFGSYGRYYEEIPMDLVIRSFSYERQPRIINYSPTSVT
                     PDANAEKDADTASAILGGFTEPSDPNLKNQYLTEYIAGAEREVMPFVSVGIKGIYRSY
                     GRVIEDFLCADDGTYCIGNPGEGIMKKIFTLDYSTQITAPKPKRTYKGIQLDATKVLS
                     NNWQGMASYIYSRLEGNYDGEYAPFTNVGADPNISAAYDYYDFFTNGSNLNVITNKGP
                     LSNDRRHQLKASGTYNTPWKLSFGVSAYWRSGTPATRYGYSDAYRRYEFFLTTRGAEG
                     RTPSNYDADLHLGYPLAVRNSQLNILLDVFNLLNTQRPVLLDQRYSFQESDNALAKSA
                     NPDYLKPIVRTPALSARLGVRWTF"
     gene            1264..2019
                     /locus_tag="VNN25_21950"
     CDS             1264..2019
                     /locus_tag="VNN25_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94255.1"
                     /translation="MKPIAIAVLSMSLAAVSHAVTIALGVGAIAPNEVIVKIDGHDSH
                     LRIAGVKPSGDPAAATFLRCLVAGRVLRVQPSSGGAAKITMLDDSVVADLVNEYFDTT
                     TKIDPCALGKAAYQPKPLHPVEATAEQTTPAAPAPTKHKGKLTFPSLPSSPAAAAQPP
                     TPFRMPADVKFPEPQAAPRPAPKSPDVGSIGTPGTMQPYTPGQAHPDTLGTSSTTPMQ
                     QGGTSTIGTAQPTNVETAQPYTPPVTPQKPPGE"
     gene            2065..2322
                     /locus_tag="VNN25_21955"
     CDS             2065..2322
                     /locus_tag="VNN25_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXH94256.1"
                     /translation="MSEISRFFGIVIAMYHREHEPAHFHARYSGQKITVRISDGYVDG
                     KFPPRALALVLEWWHLHREELAENWRLANELKELKRIRPLE"
     gene            2325..>2504
                     /locus_tag="VNN25_21960"
     CDS             2325..>2504
                     /locus_tag="VNN25_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXH94257.1"
                     /translation="MEDIVEARYVSGYTVWLRFEDGTEGEVDLSNELEGPIFEPLQDK
                     TYFSRFRVNPDTGTIE"
BASE COUNT          570 a          848 c          654 g          432 t
ORIGIN      
        1 ccgcagcaca agaacacggc catgtacctc caggaccact ggacgctgaa gaacgtaacg
       61 ctcaacgccg gtgtccgctg ggaccggcag cgcatcatcg atgcctccgg cgtcacacag
      121 atcgatctca agaaagacct ggcaccccgc ctcggattga tctgggaccc gagcggcacg
      181 aatcagacca aggtgttcgg ttcctacggc cgttattacg aagagatccc gatggatctc
      241 gtcatccgct cgttctccta cgaacgccag ccgcgcatca tcaactacag cccgaccagc
      301 gtcacgcccg acgccaacgc agagaaggat gccgataccg cgtcagccat cctcggcggc
      361 ttcaccgagc catccgatcc caatctcaag aatcaatacc tgaccgaata catcgccggc
      421 gccgagcgcg aggtcatgcc gttcgtctcc gtcgggatca aaggaatcta ccgcagctac
      481 ggacgcgtca tcgaagactt tctctgcgcc gacgacggta cgtactgcat cggcaatccc
      541 ggcgaaggga tcatgaagaa gatcttcacg ctcgattact ccacgcagat cactgcgccc
      601 aaaccgaagc gtacttacaa gggcatccag ctcgacgcca ccaaggttct ctccaacaac
      661 tggcagggta tggcctcata catctattcg cggctcgagg ggaactacga cggcgagtac
      721 gcaccgttca ccaacgtcgg cgccgacccc aacatctccg ccgcctacga ctactacgac
      781 ttcttcacca acggcagcaa cctgaacgtc atcaccaaca aaggtccgct ctcgaacgat
      841 cggcgccacc agttgaaggc gtccggcacg tacaacactc cctggaagct ttccttcggc
      901 gtttctgcgt attggcgcag cggaacgccg gccacgcgct acggctactc cgacgcctat
      961 cgccgttacg agtttttcct cacgacgcgc ggcgccgaag gccgcactcc cagcaactac
     1021 gacgccgacc tccacctcgg ttaccccctc gccgtccgaa actcccagct caacatcctg
     1081 ctcgacgtgt tcaatctcct gaacacccag cgtcctgtgc ttctcgacca gcgctacagt
     1141 ttccaggaga gcgataacgc gctggcgaag tcagcaaacc ccgactatct caaaccgatc
     1201 gtgcgcacac cggcgctttc cgcgcgcttg ggcgtacgat ggacgttcta aaggagaccc
     1261 gtcatgaagc cgatcgcgat cgcagtgttg tcgatgtcac tcgctgctgt ctcgcacgcc
     1321 gtaaccatcg cactcggagt gggtgccatc gctccgaatg aggtcatcgt caagatcgat
     1381 ggccacgatt cccatctgcg gatcgcagga gtcaaaccct ccggcgatcc ggctgcagcc
     1441 accttcctcc gctgtctcgt cgccggccgc gttctgcgcg tgcagccgtc gtccggcgga
     1501 gccgcgaaga tcacgatgct cgacgacagc gtcgtggccg atctcgtcaa cgaatacttc
     1561 gacaccacga cgaagatcga tccctgcgcg ctcggaaagg cagcgtatca accgaagccg
     1621 ctccacccgg tcgaagcaac ggccgagcag acaacgccgg ccgcaccggc gccaacgaag
     1681 cacaagggca agttgacgtt cccgtcgctc ccatcatcac ccgccgccgc tgcacagccc
     1741 cccacaccat tccgcatgcc cgcggatgtg aagttccctg aaccgcaagc cgcgccgcgc
     1801 ccagccccga agtctcccga tgttggaagc atcggcacgc ccggcacgat gcaaccctac
     1861 acacccggtc aggcgcaccc agacaccctt ggcacttcct cgacgacacc gatgcaacag
     1921 ggcggaacat ccaccatcgg aacggcccaa ccaaccaacg tagaaaccgc tcagccgtac
     1981 acgccgccgg tgacgccgca aaaaccgccg ggggagtgag agttagacgg aaggaaacgc
     2041 gacactcgtc gcatctagaa acgcatgtcc gagatcagcc gattctttgg catcgtcatc
     2101 gctatgtatc atcgtgagca tgaacccgct cactttcacg cgcggtacag cggtcaaaaa
     2161 atcactgttc gaatcagtga cggttacgtc gatgggaagt ttccacctcg cgcgttggcg
     2221 cttgtgctgg aatggtggca tcttcacaga gaggaactcg cagagaattg gcgattagcg
     2281 aacgaactga aggaactcaa gcgcattcgt cccctggagt agtcatggaa gacatcgtcg
     2341 aagctcgtta cgtgagtggg tacaccgttt ggctgcggtt tgaggacggg acggaggggg
     2401 aagttgacct ttcgaatgaa cttgaaggac cgatcttcga acccctgcaa gacaaaacct
     2461 acttcagccg gtttcgggtc aatccggata caggaacgat cgag
//
LOCUS       DATLVW010000751        15909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_528284, whole genome shotgun sequence.
ACCESSION   DATLVW010000751 DATLVW010000000
VERSION     DATLVW010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528284"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..498)
                     /locus_tag="VNN25_21965"
     CDS             complement(43..498)
                     /locus_tag="VNN25_21965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94258.1"
                     /translation="MSSVPKILVVEDDDAIRALLIAALRREPFDVHAAMHGGQALNMT
                     QATDYAVILLDLMMPTLNGIEFLEAFHQASPDTRSVIFVMTAGDDTALRHLSPNVVHG
                     IVRKPFDVPQLVAMVREVALARREQTPESAVDAVVAEADAEAARADPPN"
     gene            579..1625
                     /locus_tag="VNN25_21970"
     CDS             579..1625
                     /locus_tag="VNN25_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94259.1"
                     /translation="MAAFEKAPPEARQLLTVPIRELGLRLDQSPVAEYVRRLYAELEG
                     AGLRYFRPLCYLSDEWGCPSEEPVIGIPFYLGDPRVAHLEDAVNDIENEREIMMYLRH
                     EAGHAFNYAYELYRTDEWHELFGSFRRPYRDDYPFVPFSRDYVRHIAGWYAQKHPDED
                     FAETFAVWLDPDSHWQQRYAHWPALRKLQYVDRVAAEVRDLPPPHAAGETDITVDEME
                     QTVEEFYRGGYGADQGDESAMIAGLALDTDLTDIFLDPSKQRDGVDYRPADDLLAEHR
                     RDIIDKVYEWTGVRRTLVRALVSAVEQRLRELSLLAVRERSRSQMIELTVYITALSMT
                     FLIGKRPLRHRKVK"
     gene            1622..2638
                     /locus_tag="VNN25_21975"
     CDS             1622..2638
                     /locus_tag="VNN25_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXH94260.1"
                     /translation="MKIAILFGQFEGYDEQPNAESDAAKPKRRRRKKKTDVEAITDSL
                     RELGHETTTFAIDGRRQTLARLARSDADLFFNLIESYNGDDTMEMHFAAYLDLIGKRY
                     TGAGPQGSYLAMDKSIAKTIVRYHGLFTPYSAVMDKGRVEHAQDIQFPVIVKPAAEDA
                     SKGIDAGSVVSSIKDLFERISYIHDEFDSPALIEEYIEGREIYAAVIGNDPPEALPLV
                     ELDLSQLPKGTPKIASYEVKFDESTDAYKKTKSAVARDLDDDVVAEIQRVAVTAVRAL
                     KLRDYARIDLRLGANNRVYLIEANPNPWLDPIAEFAMAAKESGLSYTEMIDRIVKAAV
                     ARRK"
     gene            complement(2703..3137)
                     /locus_tag="VNN25_21980"
     CDS             complement(2703..3137)
                     /locus_tag="VNN25_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94261.1"
                     /translation="MKLRILAVALVLFASTAIGYARSSPNVSVNMQQLTSTNDTFYFR
                     GPVSIQYRLEVTNPTSQPVTLRSLRLESIGPGAYTIHTTNTPLKLQLGPNESRAVTIS
                     AWGRARGGFLSADEPVTIQGTAYFNSPSGSFVKLFNENIMQP"
     gene            complement(3265..6519)
                     /locus_tag="VNN25_21985"
     CDS             complement(3265..6519)
                     /locus_tag="VNN25_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXH94262.1"
                     /translation="MIERVIRWCATNRGLVIIVVAVLVAIGVWSIQNIAVDALPDLSD
                     TQVIILSKWDRSPDIIEDQVTYPITTALLGAPHVKAIRGFSDFGYSYVYVIFDEGTDT
                     YWARSRVLEYFSKVQSQLPQGVETSLGPDATGVGWVFQYALVDTTGQHSLADLRSLED
                     WHIRYELQSVPGVAEVASIGGFVRQYQITVDPNKLAGYGIALAQLSEAVRKSNNEVGG
                     RLIEWSGAEYMVRAHGYVRNKSDLEQIVLKAKDGTPVRLGDVASVQLGPEMRRGIAEL
                     DGRGEVAGAVVVMRQGENAMKVIIAVKERLAQIATSLPKGVQIVTTYDRSLLIEESIH
                     TLKHELLIQMLIVSLVILLFLWHIPSAMIPIVTLPAAVILAFIPMHLFGINANIMSLG
                     GIAVAIGALVDAAVVVVENAHKKLEVWESGGRIGDYKEVLIKAIQEVGRPSFYSLLVI
                     ALSFLPIFALEAQEGKLFKPLAFTKNFSMAIAAFLAITLDPALRLMFTKLKRYEFKPR
                     WLAGIANAVFVGRMVPEERHPISRVLFRIYGPPAHWVLRHTRLVIAAALIIVLATIPI
                     YFKLGHEFMPPLNEGVILYMPTTLPGISVAQASALLQTQDALLRQVPEVERVFGKAGR
                     ADTSTDPAPLSMAETTVILKPESQWRHKERWYSSWLPSFLQPMVRPLWPDTMSWEELV
                     NDLDRRLQLPGQTNAWTMPIRNRIDMLTTGIRTPVGVKVFGADLKQIEGIGKQLEQII
                     GRVPGSRSVYGERVAGGYFVDFDIDRAAIARYGLTIEDVQQTIMTAIGGENVSTTIEG
                     RERYPINIRYPRELRDEIDKLGRVLVATPAGAQVPLGQLANVRIIEGPAMIRDEGGKL
                     AGYVYIDVDTARRDLGSFVDDAKRAVDAQLRMPAGYTLQWSGQYEAMQRVKQRMIVVV
                     PLTLFVVAFLLLMNTRSIPKTIIVLLAVPFSAVGAVWLLWVLGYNVSIGVWVGLIALL
                     GLDAETGIFMLLYLDLAFDERVARGAMRTPDDLRESIYEGAVKRVRPKVMTVACAFFG
                     LVPIMWSNGAGADVMKRIAAPMIGGLFTSFLMELLVYPAIYYRWKWRSVREVEG"
     gene            complement(6516..7775)
                     /locus_tag="VNN25_21990"
     CDS             complement(6516..7775)
                     /locus_tag="VNN25_21990"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH94263.1"
                     /translation="MKRALPWIIAGLLAIALVVVLVVKRGGSNDADAVTQDGSGRRVT
                     AWIDPMYSQGPPHVYKSNHPGRAPDCGMKLVPVYVDDAPAANASTNVNGYSNVSVSPQ
                     RQQLIGVKLGTAELRNLSRTTRAAGRIAVDERRIAQVHTKLEGTVEALSVNFVGQPVR
                     RGDVLLSLYSPDLLATQNELLLAQRNSSDLGHTLAGAARARLRLWDMSEADIDHVVRS
                     GKPMRDVTLRSPVNGVVLAKNVVLGARVMPADTLFEVGDLSHVWVLADVYESELATLR
                     IGASAQVVANGQTLDARVTFIGPVIAAQTRTANVRLELENPSGLLKPDMYVDVVLQQP
                     VGNVVAVPDSAVMNTGTRSVVFVASANGFEPRDVITGAKVAGFYEIRSGVTAGERVVI
                     DANFLVDSESRLKAAISKVPPMTGGTP"
     gene            complement(7772..9040)
                     /locus_tag="VNN25_21995"
     CDS             complement(7772..9040)
                     /locus_tag="VNN25_21995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH94264.1"
                     /translation="MDRRHIAACLTGVFLIAARAFALDRNTDWNTPDTVVEAAIAASP
                     SLHEIDARIAAARARVAGAGTLPNPMLMAGVQNRQIDLSPDPMTMYMVGASQTFVRRD
                     RRETLQRAAALDLQQLEREREVRVAEVKRDVLIAYDEAAAAANQIAANEEIATLVAST
                     GQAARIRYETGFAPQIDIIRAKLEETNVRHAILMQRGALQQARARLRALLLLPADAEI
                     PSFALSHSMEHHGETSVASVQAPPSTAVPEAAAARAEAEITMAKLIAKPDVNLEASYG
                     FRPQQKDMFSVVARIELPVRRTVIEPRIAQATAEHDAALAQIDVVRQQLQVELGKAIA
                     QRDEAVEQINLHVDLLVPEAKLGFDSALASYQSGKTSFDSVIGALQTYRSLRVDYYDF
                     LRQLLVSEALIDAIQHGSVSTVVPEMGGAR"
     gene            complement(9110..9535)
                     /locus_tag="VNN25_22000"
     CDS             complement(9110..9535)
                     /locus_tag="VNN25_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94265.1"
                     /translation="MKLTHYARVAAPAEWLASPVRCQHLMRRFFIAIVVLSISGALPL
                     MASVGVCATTPCCRAHTASDQSIAAPSCCKETTCASATPDVQATNQVAKILVKPPVAA
                     VLVAALPSTSLRHAVAEQLAPGSPPAQQRRLAVLSTLLV"
     gene            complement(9646..10413)
                     /locus_tag="VNN25_22005"
     CDS             complement(9646..10413)
                     /locus_tag="VNN25_22005"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXH94266.1"
                     /translation="MLSIHVSDVHGLMEVIHGLQGPSLDLILHSPGGSPTAAEAVVTY
                     LRSKFSDLRVIVPYAAMSAATMVACAADSIVMGKHSFLGPIDPQILVQTALGVRMVAA
                     QAVIQQFQQAKVECQDQRNLAAWLPMLSQYGPDLLVESENFSKLSRKLVESWLKQYMF
                     KDEPEKATDIAEWLATHDNFMTHSRQLSRDVLESHGLRISKLEDDQTAQDLFLSVFHA
                     SILTLSTGTVKLMENHLGKCMATRIQVVPVQQPPANP"
     gene            complement(10607..10810)
                     /locus_tag="VNN25_22010"
     CDS             complement(10607..10810)
                     /locus_tag="VNN25_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94267.1"
                     /translation="MDTAPEEVLPELKDEIGAPSIETALAAIQFWQTLDRIYAPVRNF
                     GSQQQQVTFSVGSLPMLNLTPTR"
     gene            complement(11146..11388)
                     /locus_tag="VNN25_22015"
     CDS             complement(11146..11388)
                     /locus_tag="VNN25_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94268.1"
                     /translation="MCRSQRGQYRRGTLQIVAVEALGEGLVDGTEFSFRLLGAISGGE
                     PARKTDRRSRGQRLLLLPEYIVRALETITAEGNMTF"
     gene            11469..13919
                     /locus_tag="VNN25_22020"
     CDS             11469..13919
                     /locus_tag="VNN25_22020"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXH94269.1"
                     /translation="MTDEGRIWKRGGALLFIFFVSGLSALLYQIVWLKYLGLIFGNTV
                     YAAATLIAVFLAGLGIGAFLFGRILLRMPPLLLYAAAEALIGTLGAFSPNGFAFLNRA
                     FVAAFPSIHGSPLLMLLVRAGLAALFLLPPTILMGGTLPLLVRRFVGDREGSGKVVSA
                     LYAINTLGATTGVALAGFVLIPNVGLLATIFTAVILNYTLAIVALALLAKGRHADAPV
                     IETPPAAAIATSTLTPAAVLLASLLMGLASIADEVFWSRILVLHLGSSVYAYSLMLFS
                     FLIGIGIGSAIIERFMSRIDIAKALGIVEIALAVVLALQVHFFGKFADLLGLVVRPFI
                     AIGSTSLFPPLLTAVLIALLIPTALMGAAFPLTVRLYEINARRGEASAVGNIYLANTI
                     GCISGSLLAGFVLIRLIGSQNGLFVMSGISLCVGAWLLWRTARRARWLASIAVVIVVI
                     AFASAKKDQVILSAGLFSEENAPILLFREDVTATVTLRQMHPGFLSLELNGVNVAGTS
                     GELIGTQKLQGHIPLLMHKNPKRVLHIGFGSGGTAWAVSRHPVESITIAEISPEVLEI
                     SNARLQSVNHGVLRDRRVHAAIGDGRNYVLATQDKFDVILSDSIHPRYAGNGSLYTQD
                     YFELCRRRLLPGGVISMWLPMYSLTTHNYLQIVRAFRDVFPNTTIWYVPNVPNAFTIV
                     IGRLEDGPVPFDRIAANWTPAVANELSPIGIRTPPDLLPAFMLGPHEVERITRFVDPH
                     IDDVPSVEYESGRLLDRNGPWLQTFYLLTNNMTPLRDGVNAPGVDVESAEQRRLRLMQ
                     AHLKFIAEGIRLERAGRL"
     gene            14019..14540
                     /locus_tag="VNN25_22025"
     CDS             14019..14540
                     /locus_tag="VNN25_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94270.1"
                     /translation="MKKTLAVGFVVAMFGIVSTASAATSSGTLTVTGTVESSISLTIE
                     SAGGTTSGTGTAAASSDLGNISKFGSAPTGFTLARGASNWTLSSTVGVQVVKANLTST
                     DYTLTAQLGSAPASGVAWKLNGSTLSDSAATTLTSTGTYASTGSYSWDIVIADSAAAA
                     AIDNSINFTATSN"
     gene            14602..15096
                     /locus_tag="VNN25_22030"
     CDS             14602..15096
                     /locus_tag="VNN25_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94271.1"
                     /translation="MKRLSTLAVTLLVLLASTRPARAEKRRATAAPPDDSLAIAFIDV
                     DSGDASMTAAGSEAWIDLKTVTHAGVARERMTKMRRRIGIRLERANGLASGTATISVR
                     LESWDGRATFRLDGRQLSSAPVTIDTHLAVGPVTVHTLEIDIPISAAEGALTASMRWE
                     AIVE"
     gene            15099..15872
                     /locus_tag="VNN25_22035"
     CDS             15099..15872
                     /locus_tag="VNN25_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94272.1"
                     /translation="MRIRTSWWMGLLTLLSPALLRAGDGTLSVAPAVVMLRGAAGQST
                     TQVLTIMNSTSQPFPFEMVARDVVVRNGARSLVDAGKVAGSVAATAVFSTRAATVLPG
                     EKLRVSVTLTVPASPSVRAVVTMFHGTSKMNSHGMHVVASLGTLLTFALTDQVSANAS
                     PLEVKAPTASTNLTLAQRLANDGSEPLFAKGMLAILNGAGILIAKSALPSRRLLPGET
                     TDVRAEYPGELLRGHYRAFVTYDLQNGKTITSSGQFEVP"
BASE COUNT         3041 a         5126 c         4770 g         2972 t
ORIGIN      
        1 caacgcgctg ccgccgatcc cccgcaagtg tcagaaatgg ggttagttag gcggatcggc
       61 ccgcgcagcc tcggcgtcag cttctgcgac gactgcgtcg acggctgact ccggcgtctg
      121 ctcacgccgc gccagggcca cttcccgcac catcgccacg agttgcggaa catcgaacgg
      181 cttccgcacg atgccgtgaa ccacattcgg cgaaaggtgc cggagagcgg tgtcgtcgcc
      241 ggcggtcatc acgaagatca cggatcgcgt gtccggcgat gcttgatgga aggcttcaag
      301 gaactcgatg ccgttgagcg tcggcatcat cagatcgagg aggatgaccg cgtaatccgt
      361 tgcctgcgtc atgttcaggg cctgcccgcc gtgcatcgct gcgtgcacgt cgaacggctc
      421 gcgccgcaac gcggcgatga gtaatgcgcg aatcgcgtca tcgtcctcta ccacgaggat
      481 tttggggacc gaagacatta tccgtgtgcg ccatgcggat ttggcgccaa aaaccgctgc
      541 agtaagagtc cggcccggtt gttgctcccg aaaaggccgt ggccgctttc gaaaaagccc
      601 cgcccgaagc gcggcagttg ctgaccgttc ccatccgcga actggggctt cgcctcgatc
      661 aatcacccgt cgccgagtac gttcgacgcc tctatgccga gctcgagggc gccggtctgc
      721 gctacttccg gccgctatgt tatttgtctg acgagtgggg atgcccttcc gaggagccgg
      781 tgatcggcat tccgttctac ctcggcgatc cgcgcgtcgc ccacctcgag gacgccgtca
      841 acgacatcga gaacgagcgc gagatcatga tgtacctccg ccacgaggcc ggccacgcct
      901 tcaactacgc gtacgagctc taccggaccg acgagtggca tgagctgttc ggttcctttc
      961 gccgcccgta ccgcgacgac tacccgttcg tacccttctc gcgcgactac gtgcggcaca
     1021 tcgcgggctg gtacgcgcag aagcatccgg acgaagactt cgccgagaca ttcgcggtct
     1081 ggctcgatcc cgattcgcac tggcagcagc gttacgcgca ctggccggcg ctgcgcaagc
     1141 tgcagtacgt cgaccgcgtc gcggccgaag tccgcgatct gccgccgccg catgccgccg
     1201 gtgagaccga catcaccgtc gacgagatgg aacagacggt cgaggagttc tatcgcggcg
     1261 gatatggcgc tgatcagggc gacgaatcgg cgatgattgc cggcctcgcg ctcgacaccg
     1321 atctcacgga catctttctc gatccatcga agcagcgcga cggagtcgac taccgccccg
     1381 ccgatgacct tctcgccgag catcgccgcg acatcatcga caaggtttac gagtggaccg
     1441 gcgtccggcg cacgctcgtg cgcgcgctcg tcagcgccgt tgagcagcgc ctgcgcgagc
     1501 ttagtctgct cgccgtgcgc gaaaggagcc gttcccaaat gatcgagttg accgtttaca
     1561 tcacggcgct ctcgatgacg tttctcatcg gaaagcgccc actccgccat cggaaggtga
     1621 aatgaagatc gccattcttt tcggacaatt cgagggctat gacgaacagc ccaacgcgga
     1681 gagcgacgcc gcgaaaccga agcgccgacg aagaaagaag aagaccgacg tcgaggcgat
     1741 caccgattcg ctgcgcgagc tgggccacga gacgacgacc ttcgccatcg acggccgccg
     1801 gcaaacgctg gcacgtctgg cccgctccga cgccgatctc ttcttcaatc tcatcgagtc
     1861 gtacaacggc gacgacacga tggagatgca cttcgccgcg tatctcgatc tgatcgggaa
     1921 gcgctacacc ggcgccggtc ctcagggctc gtacctggcg atggacaaga gcatcgccaa
     1981 gaccatcgtc cgctaccacg ggctgttcac gccctactcc gcggtgatgg acaaagggcg
     2041 tgtcgagcac gctcaggaca tccagtttcc ggtcattgtg aaaccggcag ccgaggatgc
     2101 gtcgaaaggg atcgacgcag ggtcggtggt gtcatcgatc aaggatctat tcgagcgtat
     2161 ctcctacatt cacgacgagt tcgactcccc cgccctgatc gaggagtaca tcgaaggccg
     2221 cgagatctac gctgccgtca tcggcaacga tcctcccgag gcactgcctc tcgtcgagct
     2281 cgatctctcg cagctcccga aagggacgcc aaagatcgcc agctacgaag tgaaattcga
     2341 cgaaagcacc gacgcctaca agaaaacgaa atcggccgtt gcgcgcgatc ttgatgacga
     2401 cgttgtcgcc gagatccagc gcgtcgccgt cacggcagtc cgcgccctga agctccgcga
     2461 ctacgcccgc atcgacctcc gcctcggcgc caacaaccgc gtctacctca tcgaagcgaa
     2521 ccccaacccg tggctcgatc cgatcgccga gttcgcgatg gcggcgaagg agtcggggct
     2581 ttcgtacacg gagatgatcg accgcattgt gaaggccgcg gtggcgagga gaaaatagat
     2641 cacgcggaaa cgcggagggg cggagagaac ctccgcccca gcgtcatgtc agcgagcgtg
     2701 cgttacggct gcatgatgtt ttcgttgaag agcttcacga acgatccgga gggactgttg
     2761 aagtacgccg ttccttgaat cgtcaccggc tcatcggccg agaggaagcc gccgcgagcg
     2821 cgcccccagg ctgagatcgt caccgccctg ctctcgttcg gaccgagctg cagcttcagt
     2881 ggtgtgtttg tcgtgtggat cgtgtacgca ccgggtccga tcgattcgag acgcaagctc
     2941 ctcagtgtca ccggctgcga tgtggggttc gtcacttcga gccggtattg aatgctcacc
     3001 ggacctctga agtagaaggt gtcattggtc gaggtgagtt gctgcatgtt gaccgacacg
     3061 ttcggagagc tcctcgcgta gccgatcgcc gtactggcaa aaaggacgag cgcaacagca
     3121 agaattctaa gcttcatggc tcatacctcc tgccggaaat cactgcaagc gagatgctga
     3181 aaaggcctcc tcagctcgcc ctcgtaacag ctctttttca caggagccag ctcagcttcg
     3241 cgtcttcgcg tcttcgcgtg atcgttaccc ttccacctcc cgcaccgacc tccatttcca
     3301 gcggtagtaa atcgccggat acacgagcag ctccatcaga aacgaggtga acagtccgcc
     3361 gatcatcggc gcagcgatgc gcttcatcac gtcggctccc gcgccgttcg accacatgat
     3421 cggcacgagt ccgaagaagg cgcaggccac ggtcatcacc ttcggccgca cgcgcttcac
     3481 cgcgccttcg tagatcgatt cgcgcaaatc atcgggtgtg cgcatcgcac ctcgcgccac
     3541 tcgttcgtcg aacgcgagat cgaggtagag aagcatgaag atgccggtct ctgcgtcgag
     3601 cccgaggagt gcgatgagcc cgacccagac accgatggag acgttgtagc cgaggaccca
     3661 cagcagccag actgcaccaa cggcggagaa tggcacggcc agcagaacga tgatcgtctt
     3721 cggaatcgag cgcgtgttca tcaacagcag gaaggcgacc acgaacagcg tcagcggaac
     3781 gacgacgatc attcgttgct tcacgcgctg catcgcctcg tattgcccgc tccattgcag
     3841 cgtgtagcct gccggcatgc gcagttgcgc gtccacggcg cgcttggcat cgtcgacgaa
     3901 actgccgagg tcgcggcggg cggtgtcgac gtcgatgtag acgtagccgg ccaacttgcc
     3961 accttcgtca cggatcatgg ccgggccttc gatgatgcgt acgttggcga gttgcccgag
     4021 gggaacctga gcgccggccg gcgtggccac gagcacgcgt ccgagcttgt cgatctcgtc
     4081 gcgcagctcg cgcgggtagc ggatgttgat cggatagcgc tcccgccctt cgatcgtcgt
     4141 cgagacgttc tcgcctccga tcgctgtcat gatcgtctgc tgcacatcct cgatcgtcag
     4201 tccgtagcgt gcgatggccg cgcggtcgat gtcgaagtcg acgaagtagc cgccggcaac
     4261 acgctcgccg taaacgctgc gcgagccggg cacgcgcccg atgatctgct cgagctgttt
     4321 gccgatgcct tcgatctgct tgaggtccgc gccgaagacc ttcacgccga ccggcgtgcg
     4381 aatgccggtc gtcagcatgt cgatgcggtt gcggatcggc atcgtccagg cgttcgtctg
     4441 tcccggcagt tggagtcgac gatcgagatc gttgaccagc tcctcccacg acatcgtgtc
     4501 gggccagagc gggcgcacca tcggctgcag aaaggacgga agccagctcg agtaccagcg
     4561 ctctttgtgc cgccactgcg actccggctt cagaatgacg gtcgtctccg ccatcgacag
     4621 tggcgcggga tcggtggaag tgtcggcgcg ccccgctttc ccgaaaacgc gctcgacttc
     4681 gggcacctgt cgcaacagcg cgtcctgcgt ctgcagcaac gcgctcgcct gcgccacgga
     4741 gatgcccggc agcgtcgtcg gcatgtagag gatgacccct tcgttgagcg gcggcatgaa
     4801 ctcgtgtccg agcttgaagt agatcggaat ggttgccaga acaatgatca gcgccgcggc
     4861 gatgacgagc cgcgtgtggc gcagcaccca atgcgcgggc ggaccgtaga tgcggaacag
     4921 cactcgcgag atcggatgcc tctcctcggg gaccatgcgc ccgacgaaaa cagcgttggc
     4981 gataccggcc agccagcgcg gtttgaattc gtagcgcttc agcttcgtga acatcagccg
     5041 cagcgccgga tcgagcgtga tcgccaggaa cgccgcgatg gccatcgaga agttcttcgt
     5101 gaacgccagc ggcttgaaga gctttccctc ctgcgcttcg agcgcaaaga tcggcaggaa
     5161 cgaaagcgca atcacgagca gcgagtagaa actcggccgt cccacttcct gaatggcctt
     5221 gatgaggact tccttgtagt cgccgattcg cccgccgctc tcccacacct ccagcttctt
     5281 gtgcgcgttt tcgacgacga cgaccgccgc gtcgacgagt gcgccgatag cgacggcgat
     5341 cccaccgagc gacatgatgt tggcgttgat cccgaacagg tgcatgggga tgaacgcgag
     5401 aatcaccgcg gccggcagcg tgacgatcgg gatcatcgcg ctcgggatgt gccagaggaa
     5461 aagaaggatc accagcgaca cgatcagcat ctggatcagc agctcatgtt tgagcgtgtg
     5521 aatcgattcc tcgatcaaga gcgagcggtc gtaggtcgta acgatctgca cgcctttcgg
     5581 cagcgaggtg gcgatctgcg cgagccgttc tttgaccgca atgatcacct tcattgcgtt
     5641 ctcgccctgc cgcatgacca ccacggcgcc tgcgacttcg ccgcggccgt cgagctccgc
     5701 gatgccgcgc cgcatctccg gccccagttg cacgctggcg acgtcgccaa ggcggacggg
     5761 agtaccgtct ttcgctttca gcacgatctg ctcgaggtcc gatttgttgc gcacgtagcc
     5821 gtgcgcgcgg accatgtact ccgcgccgct ccactcgatc agccggccgc ccacttcgtt
     5881 gttcgacttg cgcaccgcct cactcaactg cgcgagcgcg atcccgtagc ccgccagctt
     5941 gttcggatcg acggtgatct ggtactgccg gacaaaaccg ccgatgctcg ccacttccgc
     6001 cacgcccggc accgattgca gctcgtagcg gatgtgccag tcttcgagcg agcggagatc
     6061 ggcaagcgag tgctgccctg tcgtatcgac gagcgcgtac tggaacaccc agccgacgcc
     6121 ggtggcatcg ggtccgagcg atgtctcgac gccctgcggc aactgtgatt gaactttgga
     6181 gaagtattcg agcacgcggc tgcgcgccca gtacgtgtcg gtcccttcat cgaagatgac
     6241 gtacacgtac gaatagccga agtctgaaaa cccacggatc gccttcacgt gcggcgcgcc
     6301 gaggagggcg gtggtgatcg gataggtaac ctggtcctcg atgatgtcgg ggctgcggtc
     6361 ccacttcgag agaatgatga cctgcgtgtc ggaaaggtcc ggcaacgcat cgacggcgat
     6421 gttctgaatg ctccagacgc cgatggccac gagcactgcc acgacgatga tgacgagccc
     6481 gcgattcgtc gcgcaccagc ggatgacgcg ctcgatcacg gcgtgcctcc ggtcatcggc
     6541 ggcaccttcg agatggctgc cttcaagcgc gactcggaat cgacgaggaa gttcgcatcg
     6601 atcacgacgc gctcgccagc ggtcacgccc gagcgaatct catagaagcc tgctaccttc
     6661 gcgccggtga tcacgtcgcg cggctcgaag ccgttggcag atgcgacgaa gacgaccgac
     6721 cgtgtgccgg tgttcatgac cgcgctgtca ggcacagcga cgacgtttcc gaccggctgc
     6781 tgcaacacga cgtcgacgta catatccggc ttcagaagcc cggacggatt ctcgagctcg
     6841 agccggacgt tggccgtgcg cgtctgcgct gcgatcaccg gcccgatgaa cgtcacacgg
     6901 gcgtcgagcg tctgtccgtt tgcgacgacc tgtgcgctcg caccgatgcg cagagtcgcc
     6961 agctcagatt catagacgtc cgcgagcacc cacacatgcg acagatcgcc gacctcgaag
     7021 agcgtatcgg cgggcatgac gcgcgcgccg agcacgacgt tctttgccag cacgaccccg
     7081 ttgaccggtg atcgcagcgt gacatcgcgc atcggcttgc cgcttcgcac cacgtgatcg
     7141 atatcggcct cgctcatgtc ccagagccga aggcgcgctc gtgccgcgcc ggcgagcgtg
     7201 tgcccgaggt cggaagagtt tcgctgcgca aggagcaact cattttgtgt ggccaggaga
     7261 tcggggctgt agagagagag cagcacatcg ccgcggcgaa cgggctgacc gacgaagttc
     7321 acggagagcg cttccacggt tccctccagc ttcgtgtgaa cctgcgcgat cctccgctca
     7381 tcgacggcga tccgccccgc cgcgcgcgtc gtacgcgaga ggttgcgcag ctcggcagtc
     7441 ccgagcttca cgccgatgag ctgctgtctc tgcggcgaca ccgagacgtt cgagtagccg
     7501 ttgacattcg tcgaggcatt cgcagccggc gcatcgtcaa cgtaaacagg aacgagcttc
     7561 atcccgcaat caggcgcgcg gccgggatga ttcgatttgt aaacgtgcgg cggcccctgt
     7621 gagtacatcg gatcgatcca cgcagtgacg cggcggcccg agccatcctg cgtgaccgcg
     7681 tccgcgtcat tcgagcctcc ccgctttacg accagcacga cgaccagcgc gatcgcgaga
     7741 agccctgcga tgatccatgg cagcgcgcgc ttcatcgcgc acctcccatc tccggaacga
     7801 ccgtacttac agagccgtgc tggatcgcat cgatcagcgc ttccgacacg agcagttgtc
     7861 gcaggaagtc gtaatagtcg acgcgcagcg agcgatacgt ctggagcgcg ccgatgaccg
     7921 aatcgaagga cgtcttccct gattgatacg acgccagtgc cgagtcgaac cccagcttcg
     7981 cctcgggcac caggagatcg acatgcaggt tgatctgctc gaccgcttcg tcgcgctgcg
     8041 caatcgcctt cccgagctca acctggagtt gctgccggac cacgtcgatc tgcgcaagtg
     8101 cggcgtcgtg ctccgccgtg gcctgcgcga tccgcggttc gattactgtc ctgcgaaccg
     8161 gcagctcgat cctggccaca acgctgaaca tgtccttctg ctgcggccgg aagccatacg
     8221 acgcttcgag attcacgtcg ggtttggcga tcagtttcgc catcgtgatc tcagcttccg
     8281 cccgtgccgc agccgcctcg ggcacggccg tcgaaggcgg tgcctgcacg gaagcgacac
     8341 tcgtctcgcc gtgatgctcc atcgaatgac tgagcgcgaa cgaaggaatc tccgcatccg
     8401 cgggcagcag gagcagcgca cggagccgtg cgcgcgcctg ttgcagcgcg ccgcgctgca
     8461 tgagaatcgc gtggcggacg ttcgtctctt cgagcttcgc gcgaatgatg tcgatctgcg
     8521 gcgcgaagcc ggtctcgtag cggatccgcg ctgcctggcc ggtcgaagcg accagcgtcg
     8581 caatctcctc gttggcggcg atctgattcg cagcggccgc cgcttcgtca taggcgatca
     8641 gcacgtcccg cttcacttcg gccactctta cttcgcgctc ccgttcgagc tgctgcaaat
     8701 cgagcgcagc agcgcgctgc aacgtttcgc ggcggtcgcg ccggacgaac gtctgcgacg
     8761 caccgaccat gtacatcgtc atcggatcgg gagagagatc gatctgacgg ttctgcacgc
     8821 cggccatcag catcggattc ggcagcgttc ccgcgcccgc gacacgagcg cgagcagcgg
     8881 cgatgcgcgc atcgatctcg tggagcgacg gcgacgccgc gatcgcggcc tcaacgacgg
     8941 tgtcgggggt gttccagtct gtgttccgat ctagcgcaaa cgcgcgggcg gcgatcagaa
     9001 atacgcctgt caaacaggcg gcgatatgcc ttcggtccat gattcctcac aagctcgggt
     9061 acgcggacgt agagcgtcca cgttcggaat tgagcgagcg tgatgaggat cagacgagaa
     9121 gcgtggagag caccgcgagc cgtcgctgct gcgccggagg agatccggga gcgagttgct
     9181 cggcgactgc atgccgcaaa ctggtcgagg gcaaggcagc aaccaacacc gcggcaacag
     9241 gaggcttcac caggatcttt gcgacctgat tcgtcgcctg cacgtccggc gtagcggacg
     9301 cgcaagtcgt ctctttacag caagaaggag ccgcgatcga ttgatcgctc gccgtgtgcg
     9361 cgcggcagca cggcgtagtg gcgcacaccc ctacgctggc catcagcgga agggctccgc
     9421 tgatcgatag cacaacgatc gcgatgaaaa accggcgcat caggtgttgg caacggacgg
     9481 gcgaggccag ccattccgcc ggagcagcga ctcgtgcgta gtgggtcagt ttcatgttac
     9541 agcctgcgcc gcctcgcatc agcgtgattt gatgagcgcg atcaagtagg gccggtccgt
     9601 gattctcgcc gccatcgcgg gcgtgcagcc gatcgtctga atgtatcaag gattggcagg
     9661 cggttgttgg acaggcacga cttgaatccg tgttgccatg cacttaccta aatgattctc
     9721 catcaacttc acggtacctg tcgaaagcgt gagaattgac gcgtggaaca ctgataggaa
     9781 cagatcctgg gcagtttggt cgtcttccaa cttactaatt ctaagaccgt ggctctctaa
     9841 cacatcgcgt gataactgtc gcgagtgcgt catgaagtta tcgtgcgtag ccaaccattc
     9901 agcaatgtcg gtcgcctttt ccggctcgtc cttgaacatg tattgcttta gccagctctc
     9961 gactaatttg cgcgagagct tcgagaaatt ttcggactca actagaagat cagggccgta
    10021 ttgactgagc ataggaagcc acgcagccaa attgcgttga tcttggcact ctacctttgc
    10081 ctgctgaaac tgttgaatca cggcctgagc cgcaaccatt cgcacgccga gcgcagtctg
    10141 aactagaatc tgtggatcga ttgggccgag gaatgaatgc tttcccataa cgatactgtc
    10201 ggctgcacac gcaaccattg tggccgcaga cattgctgcg tacggaacaa tgacccgcag
    10261 atccgagaac ttactacgaa ggtatgtgac gaccgcttcg gctgccgttg gcgatccacc
    10321 cggactgtga aggattagat caagcgatgg tccctgcaac ccatggataa cctccatcaa
    10381 accgtgaaca tcgctgacgt gaatcgaaag catgtccggc ggggaattcg gagggggatt
    10441 catccacgcg gtcgcgtaga gtattaggtt cctgcgggtg tgtgcgtgag cgagcgctag
    10501 gtattttcgc cgaactgtgt cgaagtcagt taggccgctg ggaagcgtag actgagccag
    10561 ttctttgaga atttcacccc aggtcggcac gtttaagcgc tctcgcctat cgggtggggg
    10621 tgaggttgag catcggtaag gagccgacac taaaggttac ttgttgctgc tgcgatccga
    10681 agttgcgaac tggcgcgtag attcgatcaa gtgtctgcca gaactgaatg gcagccaacg
    10741 cagtttcaat cgacggagcg cctatctcat ccttgagttc cggaagtact tcttcgggcg
    10801 ctgtgtccat tctgttctca ggcatgcatc caccccgtta tcccagtatc agcgggccgt
    10861 gccggattcc tcatagcgaa gatgagcaaa tatgagtcca gacgcgctcc tcgtcaagtg
    10921 aaaaaacaca caaacatggc aactcggata gccactcgtc cgaaagtgta accggtttca
    10981 tcacatgaaa gtgtatcgcg agtctggact gccttctcct cgacagcggg aaagagtact
    11041 ccttaccggg gaacaggtac gctcgccgat agccgggaat cgtcccttgc atccgcgtcg
    11101 agtttgcgat cctgcgaagt cgatcagctc gccgtgcgag gcagcttaaa acgtcatgtt
    11161 gccctcggct gtgatcgtct ccaacgctcg cacgatgtat tcgggaagca agagaagacg
    11221 ctgaccccgc gaacgacggt cagtcttgcg cgctggctcg cctcccgaga tcgcgcctaa
    11281 gaggcggaac gaaaactcag tgccatctac gagaccttca ccgagggctt cgactgcaac
    11341 gatttgcagc gtgccgcggc ggtactgtcc gcgttgcgag cggcacatca cactcccgcc
    11401 tctgtgtcct gaacagcatg gaaaaagacc ccccgcgtct ccgcgtgaaa ctgttacgct
    11461 tcagctcgat gacagacgag ggacgtatct ggaagagggg cggtgctctc ctcttcatct
    11521 tcttcgtctc ggggctgagc gctcttctct accagatcgt ctggctgaag tacctcgggc
    11581 tgatcttcgg caacacggtc tacgcggcgg ccacactcat cgccgtcttt ctggccggac
    11641 tcggcatcgg cgcctttctc ttcggccgca tccttcttcg catgccgccg ctgcttctct
    11701 acgcggcagc cgaagctctc atcggcaccc tcggcgcgtt ctcgccgaac ggattcgcgt
    11761 tcttgaatcg cgcgttcgtc gccgcctttc catcgattca tggaagccct ctcttgatgt
    11821 tgctcgttcg cgccgggctg gcggcgctct tccttcttcc gccgacgatt ctcatgggcg
    11881 gaacgctgcc actgctcgtt cgccggttcg tcggcgatcg cgaaggaagc ggcaaggtcg
    11941 tctccgcgct ctacgccatc aataccctcg gcgcgacaac cggcgtggcg ctggccggat
    12001 tcgtcctcat cccgaacgtc ggacttctcg ccacgatctt caccgccgtg atcctcaact
    12061 acacgctggc gatcgtggcg cttgcacttc tcgccaaggg gcgccacgcc gatgctcccg
    12121 tcatcgagac gccaccggcc gctgccattg cgacgagcac gctgacgccg gccgccgtgc
    12181 ttctcgcttc cctgctcatg ggactcgctt ccatcgcgga cgaagtcttc tggtcgcgga
    12241 ttctcgtcct ccacctcggt agcagtgtct acgcctactc gttgatgctg ttctcgttcc
    12301 tcatcggcat cggaatcggc agcgccatca tcgagcgctt catgtcgcgg atcgacatcg
    12361 ccaaggcact cggcatcgtc gaaatcgcgc tcgccgtggt actggcgctg caggttcact
    12421 tcttcggaaa gttcgccgat ctgctcggac tcgtggtccg gccgttcatc gcgatcggat
    12481 cgacctcgct ctttccgccg cttctcaccg cggtcttgat cgcgctcctg atcccgactg
    12541 cgctcatggg agcggccttc ccgctgacgg tgcgcttgta tgagatcaac gcgcgccggg
    12601 gagaggcgtc cgcagtcgga aacatctatc tggcgaatac gatcggatgc atcagcggct
    12661 cgctcctcgc gggattcgtc ctcatccgtc tcatcggttc acagaacggc ctcttcgtga
    12721 tgagcggcat cagtctctgc gtcggcgcgt ggctcctctg gcgaaccgcg cgccgtgcac
    12781 gatggctcgc gtcgatcgcc gtagtcatcg ttgtcatcgc gttcgcgagc gcaaagaaag
    12841 accaggtcat tctttccgcg ggtctcttct ccgaggagaa cgcaccgatc ctgctgttcc
    12901 gcgaggatgt caccgccacg gtcacgctgc ggcaaatgca tcccggattt ctgtcgctcg
    12961 agctcaacgg tgtcaacgtc gccggcacct cgggagagtt gatcggcacg cagaaactgc
    13021 agggacacat accgttgctg atgcacaaga accccaaacg cgtgcttcac atcgggttcg
    13081 gcagcggcgg cacggcgtgg gcagtgtcgc ggcatccggt tgagagcatc acgatcgcgg
    13141 agatctcccc cgaggtgctg gagatctcga acgcacggct gcagtcggtg aaccacggcg
    13201 tactgcgcga ccggcgcgtc cacgctgcga tcggcgacgg acgcaactac gtcctcgcca
    13261 cgcaggacaa attcgacgtc atcctctccg actccatcca cccccgttac gccggcaacg
    13321 gctcgctcta cacgcaagac tacttcgagc tttgccgccg gcgcctgctt ccgggcggcg
    13381 tgatctcgat gtggctgccg atgtactcgc tcaccacgca caactatctg cagatcgttc
    13441 gcgcctttcg cgacgtcttc cccaacacga cgatctggta cgtgccgaac gtcccgaacg
    13501 cattcacgat cgtcatcggc cgtctcgagg atggccccgt ccccttcgac cgcatcgccg
    13561 cgaactggac gccggccgta gcgaacgagc tatcgccgat cggcatacga accccgcccg
    13621 acctcctacc cgctttcatg ctcggtcccc acgaagtcga gcgcatcacc agattcgtcg
    13681 atccccacat cgacgacgtc ccctcggtcg aatacgaaag cggccgcctc ctcgaccgca
    13741 acggtccctg gctccaaacc ttctacctgc tgacgaacaa catgacgccc ctccgtgacg
    13801 gcgtgaacgc ccccggcgtc gacgtcgagt ccgccgagca gaggcgcctg cgcctcatgc
    13861 aagcgcacct gaagttcatc gccgaaggca tccgcctcga gcgcgccggg cgactgtagc
    13921 catccaaagc aaccgcatct ttttttgagc ctctgtattt tttcaccttc tccctgccac
    13981 tcatgcgcat aggcagcacg aaaaaaggga gaaagaaaat gaaaaagaca ctggcagttg
    14041 gttttgtagt agcgatgttc gggatcgttt cgacggcctc ggcagcaacc tcgagcggca
    14101 cgctcaccgt gaccggtacc gtggaaagct cgatcagcct gacgatcgaa tccgcgggcg
    14161 gcacgacttc gggcacgggc acagcggcgg caagctcaga tctcggcaac atcagcaagt
    14221 tcggctcggc gccgaccggc ttcacgctcg cacgtggcgc cagcaactgg accctttcgt
    14281 cgaccgtcgg cgtccaggtc gtcaaagcga atctcacgag caccgactac acgctcaccg
    14341 cgcagctcgg aagcgcgccg gcctcgggcg ttgcctggaa gctcaacgga agcacgctga
    14401 gcgattcggc tgcgacgacg ctcacctcga ccggcaccta cgcgtcgacc ggtagctact
    14461 cgtgggacat cgtgatcgcc gacagtgccg cagccgccgc gatcgacaac agcatcaact
    14521 tcacggctac ctccaactaa ccgcaaaaca ccaggaaggc cggtgccggg agacttccct
    14581 gcaccggccc ttggactatt catgaaacgc ctcagcacac tcgccgtcac tcttctcgtt
    14641 ctgctcgcaa gcacgcggcc ggcgcgggcg gagaagcgac gcgcgacggc ggcgccgccc
    14701 gatgactcgt tggcaatcgc attcatcgac gtcgattcgg gtgacgcgtc gatgaccgcc
    14761 gccggctccg aagcgtggat cgatctcaaa acggtcactc acgccggcgt ggccagagag
    14821 cggatgacga agatgcgccg ccggatcgga atccggctcg agcgcgcgaa cgggctcgca
    14881 tcgggaaccg caacgatcag cgtccggctc gagtcgtggg acggacgagc aacctttcgc
    14941 ctcgacggca ggcagctcag cagcgcgcca gtgacgatcg acacgcacct cgcagtcggg
    15001 ccagtgacgg tccacacact cgaaatcgac attccaatct cggccgcaga gggcgcacta
    15061 acggcctcga tgcgctggga agcgatcgtg gagtaatcat gagaatcagg acatcgtggt
    15121 ggatgggact tctcacgctg ctgtcgccgg cgcttctgcg cgccggcgat ggaacgctct
    15181 cggtggcgcc cgcagtcgtg atgctgcgcg gagccgcggg tcagagcacg acacaagtcc
    15241 tcacgatcat gaactccaca tcccagccgt ttccgttcga gatggtggcc agggacgtgg
    15301 tcgtccgcaa cggcgcacgc tcgctcgtcg atgcaggcaa ggtcgcaggc agcgttgccg
    15361 caacggcggt cttctcaacg cgagccgcaa cggtgctgcc cggcgagaaa ctgagagtga
    15421 gcgtgacgct gacagttccg gcaagtccat ccgttcgtgc cgtagtgacg atgtttcacg
    15481 gaacgtcgaa gatgaacagc cacggcatgc acgtcgtggc atcactcggc acgctgctga
    15541 cgttcgcact aacggaccag gtcagcgcaa acgcatcgcc actcgaagtg aaggcgccta
    15601 ccgcgagcac gaacctcacg cttgcacagc ggctcgcgaa cgacggcagt gaaccgctgt
    15661 tcgccaaagg catgctggcc atcctcaacg gagccggcat cctgatcgcg aagagcgcgc
    15721 taccgtcgcg tcgtctgctg cccggggaga caacggacgt tcgcgcggag tatccgggcg
    15781 agttgctccg agggcactac cgcgcatttg ttacctacga ccttcagaac ggaaagacga
    15841 tcacgtcgtc gggacagttc gaggtcccat gagacgcgcc gcgctgggac tgacgctttc
    15901 gctgctcgc
//
LOCUS       DATLVW010000752         6050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_320594, whole genome shotgun sequence.
ACCESSION   DATLVW010000752 DATLVW010000000
VERSION     DATLVW010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320594"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VNN25_22040"
     CDS             complement(<1..534)
                     /locus_tag="VNN25_22040"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HXH94273.1"
                     /translation="MIDLSADLGEGAPDEPEIWPLITSANVACGGHYGDIDSMTHAVR
                     RARDLGVRVGAHPSYPDRSNFGRRSMTMPPQMLRSSIIEQINALRDIAGKLTHVKPHG
                     ALYNDAHKDRALAEIVIDAIRAVDPSLPIVCSDSSQMADAARASGIPVIREAFADRRY
                     NADGSLVARSEPGSLLDV"
     gene            complement(531..1739)
                     /locus_tag="VNN25_22045"
     CDS             complement(531..1739)
                     /locus_tag="VNN25_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH94274.1"
                     /translation="MSSLRSVDRRAWHALFAAQLGWMLDAMDFLLFSFAVIPIREEFH
                     LTNSTMGALTSVALIASAIGGMVFGRLADRLGRVRALTLSILLYSAATAGLATSAALW
                     QLVAWRILVGLGMGGEWSAGSVLVAETWPAEHRGKAMGLMQSGWAIGALIAAAVSALT
                     IERYGWRVLFLIGALPAIAAFFIRRNVEEPQVWRERPAEASRWVEMFETPFLRRTAIA
                     TLLASSVLMAFWGVTSWLPTFLATPLAKGGAGLTVTTSASWLIALQVGAFFGYISFGW
                     IADRIGRRPAFTFFMIASTILVPLFAFYARTPTLLLVLGPLLGFFAHGYFSLFGAMLA
                     ELFPTRIRAGAQGFCYNGGRFASAAATFAIGKASLTHSFATVITCAALFFAVGAALVW
                     LLPETKGAEL"
     gene            complement(1952..3328)
                     /locus_tag="VNN25_22050"
     CDS             complement(1952..3328)
                     /locus_tag="VNN25_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94275.1"
                     /translation="MPKPLAAVITQIKAGKIPPVILVGGSSDYLAEDAFRILRDAIVA
                     AKPGVSVESYEAGSELAAILDSYRTMSLFGSARLIVVSDVNAFVSAKELASLYDKSLA
                     DWKSAKTDRKRSSAAAKLLHVLGLVGADFDMTDREIATALGAPLDPLLTDLLAFCRTT
                     GKKASRGEDDAALLIEAITRGGAPNTWLLMRSGEVPRESATVELIDRNGAVVVMDLTR
                     ETFSGALDRAIAEVAEEAQVRFDPRALGMLRQRLGIERLLADKFAKDVPDLRPAVSEA
                     ERLATLAGTGGRVTAEMVEREVAVVEGGARYEFGSLFTEGKIMEAIAKLRDLVAQARR
                     DDPKAPLDIQYGKFLFPLADEVRQMIGVVAFARTNRIDLRAPMNYNRFKDTLADRLGD
                     SLKANGLVRQKPHPFPLHKKWEAARNQTEASLFRALAALADLEIKRKSGGIPADLGIE
                     TFLLQRLR"
     gene            complement(3338..3916)
                     /locus_tag="VNN25_22055"
     CDS             complement(3338..3916)
                     /locus_tag="VNN25_22055"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXH94276.1"
                     /translation="MSPELLLHKFGYLAVFIGTFLEGETILVMGGFFAERGYMTLPLV
                     IITAFGGSYVGHVCWFWLGRTQGVRLLDRFPRLKKHFGKGIRLFERYGAPAIFITQWL
                     YGLRITCAVIIGISKISTIKFLLYEALTCIVWAIVIGTAGFYFGRAVERVLGRAAHVE
                     KWGLLVLVVVGIGVWAYHRWKDKREEPDTPAC"
     gene            4000..4563
                     /locus_tag="VNN25_22060"
     CDS             4000..4563
                     /locus_tag="VNN25_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94277.1"
                     /translation="MKPMMKPIALTASLILAALSLYANDPQSKPKEAAKAQSATPAPA
                     ALSVSNATGVDSPLVRAAKAAHKSGIKASTVITNETLIRSGGHFTTAASQQPIAPVKG
                     VPGPSEEQWLADRRRKEIEAVTAAIAAAKSEQQKKLAAARLEQLMEGDTPEGMLNDQP
                     GQMEGPVQTMTPSTPTTMQPTTPKPPL"
     gene            complement(5158..>6050)
                     /locus_tag="VNN25_22065"
     CDS             complement(5158..>6050)
                     /locus_tag="VNN25_22065"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXH94278.1"
                     /translation="EIEQTRHNPIYLPTIALDARVRATSSLAEAASFSDVLFMMVPSH
                     HFRGVLAQIRPHLQSQVSIVSGTKGIENDTLMRMSEVSEIELKDALRGFAVLSGPTFA
                     LETARGDPTAAVVASTDLALAEQVQHAMSCPTFRLYHSNDVAGVELAGSLKNVIAIAA
                     GVLEGLGLGSNTTAALITRGLHEMTKLAIALGGRPETLAGLAGMGDLVLTCTGSLSRN
                     RSVGVALGKGKALAEILSETRAVAEGVRTSKAARELAERHHIEMPITVEMVRVLHENE
                     SPRVALQRLMTRSLKAEAAL"
BASE COUNT         1181 a         1925 c         1931 g         1013 t
ORIGIN      
        1 gacatcgagc agcgaaccgg gctcgcttcg ggcgacgagc gacccatcgg cgttgtatcg
       61 gcgatcggcg aatgcttcgc ggatgacggg gatgccagag gcgcgcgcgg cgtcggccat
      121 ctgcgaggaa tcgctgcaga cgatcggcag cgatggatcg actgcgcgaa tcgcatcgat
      181 cacgatctcc gcgagcgccc gatctttgtg cgcgtcgttg tagagcgcgc cgtggggttt
      241 gacgtgggtc aacttgcccg cgatgtcgcg cagcgcattg atttgctcaa tgatcgaaga
      301 acgcagcatt tggggcggca tcgtcatcga cctgcgtccg aagttcgaac ggtcgggata
      361 cgacggatgc gcgccgacgc ggacgccgag gtcgcgcgcg cggcggacgg cgtgcgtcat
      421 cgaatcgatg tcgccgtagt ggccgccgca ggcgacgttc gcggatgtga tgagcggcca
      481 gatctccggc tcgtcgggag caccttcgcc gaggtcggcg ctgagatcga tcacaattcg
      541 gctcctttcg tttcgggcag cagccagacc agcgccgcgc cgaccgcgaa aaagagcgcg
      601 gcgcaggtga tgaccgtcgc gaagctgtgc gtcaacgatg ctttgccgat cgcgaacgtc
      661 gcggccgcgg atgcgaagcg gccgccgttg tagcagaagc cctgcgcgcc ggcccggatg
      721 cgcgtcggga acagctccgc gagcatcgca ccgaagagcg agaagtagcc gtgcgcgaag
      781 aagccgagga gcggcccgag gacaaggagc aaggtcggtg tgcgggcgta gaaggcgaag
      841 agcggaacga ggatcgtcga cgcgatcatg aagaatgtga acgcgggacg gcgcccgatg
      901 cggtccgcga tccagccaaa actgatgtag ccgaagaacg cgccgacctg cagcgcaatg
      961 agccagctcg ccgacgttgt gaccgtgaga ccggcgccac ccttcgccaa tggcgtggcc
     1021 aggaacgtcg gcagccacga ggtcacgccc cagaaggcca tcagcacgct gctggcgaga
     1081 agcgtcgcga tcgccgttcg gcgcaggaat ggcgtctcga acatctcaac ccaccgcgac
     1141 gcctcggccg gccgttcgcg ccagacttgc ggctcttcga cgttgcggcg gatgaagaac
     1201 gcggcgatgg ccggaagcgc accgatgaga aacagcacgc gccagccgta gcgctcgatg
     1261 gtcagggccg acaccgcggc ggcgatcaac gcgccaatcg cccagccgga ctgcatgagc
     1321 cccatcgctt tgccgcgatg ctccgccggc cacgtctctg ccacgagcac ggagccggcg
     1381 gaccactcgc cgcccatacc gagtccgaca aggatgcgcc acgccacgag ttgccagagc
     1441 gcggcgctcg tggccagacc ggcggtagcg gcggagtaga gcaggatcga gagcgtcagg
     1501 gcgcgcacgc ggccgagacg gtcggcaagg cgtccaaaga ccatcccgcc gatggcgctg
     1561 gcgatcagcg cgaccgaggt gagcgcgccc atcgtggagt tcgtgaggtg gaactcttcg
     1621 cggatcggaa tgaccgcgaa gctgaacagc agaaagtcca tcgcgtcgag catccagccg
     1681 agctgcgcgg caaagagcgc atgccacgcg cggcggtcga cggagcgaag cgacgacatg
     1741 aagggcgcag tttagcggtg aagcagagat cagggaacag agatcaggga acagagatca
     1801 ggatgaagcg gagatcagag actagagaca aacaacctga tctcttgtct cttgtctctt
     1861 gtctctcgtc tctgatctct gcattagccg aaagcgacaa gaggtcagag atcaggagca
     1921 aaaagcccga tctcttttct ctgatctctg cttatctcaa ccgctgcagc aggaacgtct
     1981 cgatcccgag gtcggccggg atcccccccg acttccgctt gatctcgagg tcggccagtg
     2041 cggccagcgc gcgaaacaga ctcgcctccg tctggttccg cgccgcttcc cactttttgt
     2101 gcagcgggaa gggatgcggc ttctggcgta ccaggccgtt ggctttgagc gagtcgccga
     2161 ggcggtcggc cagcgtgtct ttgaatcggt tgtagttcat cggggcgcgg agatcgatgc
     2221 ggttcgtccg cgcgaaggcg actacgccga tcatctggcg cacttcgtcg gcgagcggga
     2281 agaggaactt gccgtactgg atgtcaagcg gcgctttcgg atcgtcacgt cgggcttgcg
     2341 ccacgagatc gcgcagtttg gcgatcgctt ccatgatctt cccttccgtg aacagcgatc
     2401 cgaactcgta ccgtgcaccg ccttcaacga cagcaacttc gcgctccacc atctccgccg
     2461 tcacacgacc acccgttcct gcgagcgtgg ccagccgctc ggcctcggac acggcagggc
     2521 gcaggtcggg gacatctttc gcgaacttgt cggccagcag ccgctcgatg ccgagacgtt
     2581 gccgcagcat ccccagcgcc ctcgggtcga agcgcacttg cgcctcctcc gccacctcgg
     2641 caatcgcgcg gtcgagcgcg ccggagaacg tctcccgcgt cagatccatg acgacgacgg
     2701 cgccgttgcg gtcgatcagc tcgaccgtgg ccgactcgcg cggcacctcg ccgctgcgca
     2761 tcagcagcca ggtgttcggc gcgccgccac gcgtgatcgc ttcgatgagg agggccgcat
     2821 catcttcacc gcgcgatgcc ttcttccctg tcgtgcggca gaacgcgagc aaatccgtga
     2881 gcagcggatc gagcggcgcg ccgagggccg tggcgatctc gcgatcggtc atgtcgaagt
     2941 cagcgccaac gagtccgagc acgtggagga gcttggccgc tgccgacgag cgcttcctgt
     3001 ccgtcttcgc cgatttccag tccgcgagcg acttgtcgta cagcgaggcc agctctttcg
     3061 cagagacgaa cgcgtttacg tcggagacga cgatcaggcg cgccgatccg aagagcgaca
     3121 tcgtccgata cgagtcgagg atcgcggcga gctcgctgcc ggcttcgtac gattcgaccg
     3181 acactcccgg cttcgctgcg acgatcgcgt cgcgcagaat acgaaacgca tcctcggcca
     3241 gatagtcgct cgatccgccg acaaggatca ccggcgggat ctttcccgct ttgatctggg
     3301 tgatgacggc tgccagtggc ttaggcatgg acggtttcta acacgccggc gtgtccggtt
     3361 cttcgcgctt gtccttccag cggtgataag cccagacccc gatgccaacg acgacgagca
     3421 ccagcagacc ccatttctcg acgtgagccg cgcggcccag tacacgctcc accgcacgtc
     3481 cgaagtagaa gcctgccgtg ccgatcacga ttgcccagac gatgcacgtc agcgcctcgt
     3541 agagcagaaa cttgatcgtc gagatcttcg aaatgccgat gatcaccgcg caggtgatgc
     3601 gcagcccgta aagccactga gtgatgaaga tcgccggtgc gccgtagcgc tcgaacaggc
     3661 gaatcccttt cccgaagtgc tttttcagcc tcgggaagcg gtcgagcagc cgcacccctt
     3721 gcgtgcggcc gagccagaac cagcagacat gcccgacgta ggacccgccg aaggccgtga
     3781 tgatcaccag cgggagcgtc atgtacccgc gctccgcgaa gaagccgccc atcaccagga
     3841 tcgtctcccc ttcgaggaac gttcctatga agacggcgag gtatccaaac ttgtggagca
     3901 gtagctcggg cgacattcaa acccgcagag tgcaagattc ggggtatctc atgaaatttg
     3961 gcgggcgaag cgtaacattg aaccttaccg gaggccttca tgaagccgat gatgaagccg
     4021 atcgctctca cagccagcct gatcctggct gccctttccc tgtacgcaaa cgacccccag
     4081 tccaagccaa aagaggccgc aaaggcacaa tccgccactc ctgctccggc ggcgttgtcc
     4141 gtctcgaacg ccacgggcgt cgactcgccg ctggtacgcg ccgccaaggc cgcccacaaa
     4201 tctggaatca aggcctcgac ggtcatcacg aacgagactc tcatccgctc gggggggcac
     4261 ttcacgaccg ccgcgtccca acagccgatc gcacccgtga aaggggttcc cggtccgtcc
     4321 gaagagcagt ggcttgctga ccgacgaaga aaggaaatcg aggcagtgac ggcagccata
     4381 gcagcggcca agtccgaaca gcagaagaag ctcgctgccg cgcggctcga acagctcatg
     4441 gaaggggata ctccggaagg aatgctgaac gatcagccgg ggcagatgga aggtccggta
     4501 cagacaatga caccgtcaac gccgacgacg atgcagccga cgacgccgaa gccgccgctc
     4561 taacccggct gctcaaagcc agcttcgaac ggctgccgcg gcatcgccac tgtctgcgtg
     4621 ccggacgttc gaggctcccg cgactttgcg agtgtgtggc gctcagacgc tcatgactcg
     4681 cggcgggctg tgaatgacat ccgccgtcat agcgaacgtc tgagcgtcag atattcgatg
     4741 ctacgaactt cgcctcgcga agtaaccgcg tcatcgcgag cgtctggccg tcagacattc
     4801 gcgggctccg ccgacattgc gaatgtctga cgctcagata ttcatgacca gcggccagct
     4861 cttgaatgac ttccgcgttg cagcggacgt cggagcgtca gatattcgat gctgcgaagt
     4921 tgtctcgcgg aataaccgcg accgtctcaa gcaccattgg ggccaggcct tcgctcgttt
     4981 gtggactgca aatcattgaa aaacggccac ttgtggcggc tcatgggaga ctctcgcaac
     5041 ggcgctcagt tattgggatc acgagtggtc gaaaactgga aggttgcccg ggcttttcgc
     5101 gtgcagcacg ccacctcgga ttggagagct gggggccggg ggcgtgagaa atgcgggcta
     5161 aagcgccgct tccgccttga gcgacctcgt catcaggcgc tgcaaggcca cccgcggcga
     5221 ttcgttttcg tgaagcacgc gaaccatttc gacggtgatc ggcatttcga tgtgatgccg
     5281 ttcggcaagc tctctggccg ctttcgatgt cctcacgcct tccgccaccg cccgcgtctc
     5341 ggacaggatt tcagcaagcg ctttcccctt gccgagggcg acgccgacgc tgcgattgcg
     5401 ggaaagcgag cccgtgcagg tgaggacgag atcgcccatc cctgccaatc ccgccagggt
     5461 ctccggacgc ccaccgaggg cgatggcgag cttcgtcatc tcgtgtagcc cgcgggtgat
     5521 cagagccgca gtcgtgttcg atcccagccc gagtccttcg agcactccgg cagcgatggc
     5581 gatgacgttc ttcagcgagc cggcgagctc gacaccggca acgtcgttcg agtggtagag
     5641 gcggaacgtc ggacacgaca tcgcgtgctg cacctgctcc gcaagcgcga gatcggtcga
     5701 cgccacgacg gcggccgttg gatcgccgcg ggccgtttcg agggcaaacg tcggtcctga
     5761 cagaacggca aagccgcgaa gcgcgtcctt caactcgatc tccgagacct cggacatccg
     5821 catcagcgtg tcgttctcga ttcccttcgt gccgctgacg atcgagactt ggctttgcag
     5881 gtgcggtcga atctgcgcaa gcacccctcg gaagtggtgg ctcggcacca tcatgaagag
     5941 aacgtcgctg aacgaggccg cctcggcaag cgagctcgtg gcccgcaccc gagcgtcgag
     6001 agcaatggtc ggcagataga ttggattgtg acgcgtctgc tcaatctcgg
//
LOCUS       DATLVW010000753         6189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_30868, whole genome shotgun sequence.
ACCESSION   DATLVW010000753 DATLVW010000000
VERSION     DATLVW010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30868"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1244)
                     /locus_tag="VNN25_22070"
     CDS             complement(21..1244)
                     /locus_tag="VNN25_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXH94279.1"
                     /translation="MTVAPEDTVSTFAISRRGQAMPASPIRKLAPLAELAASRGTKVY
                     HLNIGQPDIETPACMMERLKQIEDKVLQYSPSTGTPAFLDSLRHYYQRRLGIPIETRQ
                     ILATTGGSEALLFAFLACANEGDDVLLIEPFYANYRAFTTMAGLNIVPVTSRGRDGFH
                     LPSRAMFERALTPRTRIVVLCNPSNPTGTVYTRDELEMLASFCRDHGLFLISDEVYRE
                     FVYDGRKAISALELRDASEFVVVVDSLSKRYSACGIRLGALVTRNGEVYDACLRMAQG
                     RLSPPGLAQFIAVGAQGLGDDYTRDVVAEYERRRDVLYNGLHAIPGVELEKPEGAFYC
                     VPRLPVDDAEDFAVWLLTDFEHEGGTVMVSPASGFYASSGLGKREIRIAYVLNEEHLR
                     TSIELLRRALETYRA"
     gene            1333..2436
                     /locus_tag="VNN25_22075"
     CDS             1333..2436
                     /locus_tag="VNN25_22075"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXH94280.1"
                     /translation="MKRLAFALLVLSLTSATFAADEPEKPKAPAEKPAPNLGARADKL
                     IKDALPVCAEEMKESRVALTHKLPENMIGAVIREESKHSPCEGQWVAAISNEGGFFMG
                     TPWFLDDTEGTLEVRLKSFTLQYMKQVFEPTIDRTKTREGLFHVTLTQAVDGGGKLPL
                     EGEIDPAGTVLFIGHFVPMSVDYHTSRLKALDHFLSLSPTTGSAKPDVTVIEFSDFEC
                     PSCQHAAGYLKPILTAHGDRVRYIRYDTPLVSIHPWALPAAIAGRAIWHQKPDLFWKY
                     KEQVYENQEKLSAFTIDDFTRGFASDHDLDMKKYDADVNSPELRTEIINGAGNAFTND
                     VRATPTYIVNGAFVDSGVDGKGLVDYVNGLLKK"
     gene            2460..2897
                     /locus_tag="VNN25_22080"
     CDS             2460..2897
                     /locus_tag="VNN25_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXH94281.1"
                     /translation="MDVDAQVEIDRLTDSFFALYSNRDGVRPDLGRIFDLFVPNGLIA
                     KCTGPDPEISTLHEFIAPRQELLTNGSLTDFSESETSKHTQIFGNIAQRISTYQKSGC
                     LDGVAFTARGVKTFQFLWTRTGWRILSVSWDDEREGLAVEELS"
     gene            complement(3191..3805)
                     /locus_tag="VNN25_22085"
     CDS             complement(3191..3805)
                     /locus_tag="VNN25_22085"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXH94282.1"
                     /translation="MPDLRPPTPDTRPPGFDIAALARLRTILSTRPAIEIEAPHLKRA
                     AVLIPLVPHDHGWCILFSLRSINLAAHSGQISFPGGGVESGEALEAAAIREAHEEVGI
                     ATERVEVIGRLDDLVTHSGFLVAPFVGVIDQRIDYVLQASEVDEVFEVPIEALLSPQQ
                     PEVRYVPFRNKQYPAYFYRYEQYEIWGLTGRILKHFLDFVWRAV"
     gene            complement(3832..5268)
                     /locus_tag="VNN25_22090"
     CDS             complement(3832..5268)
                     /locus_tag="VNN25_22090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94283.1"
                     /translation="MKIALYSPLPPTRTGVAHYAAMLLPALREKIEVVTISEPLSDPR
                     PPTPDTRIYFLGNNPHHAWIYEEAMRTPGVIVLHDLVLHHLIVEMTLARGDIDGYVAA
                     LAANHGEAGAAWARGRAAGLHSEMGNFLMPASVDVANRSLAVIVHNHYAADRLRSFGV
                     TAPIHLVPHPFEPQPSARGQRDAVRAQHAFSSGNRVIGLFGFLTSAKRADVVLAAFAQ
                     ARAQQPRLRLLVVGEPAPNVDVDSLRGDGITFTGYVPDDEFAAYFAAVDRLVNLRYPS
                     AGETSGTLIRAFEAGRPVAVSDYAQFAELPDDCVVKIPFGDREATTLAGFFVREIPDP
                     SKAQTAWLAENASMDLTVQGYLAALNDRSRDTRTAITRTLPLFPKIEAGLRGEKLALR
                     NAGEFTLRTHSYGQPGYRLMMLTFEGDKVVDDRWIELPRDLRPGDIAEVDLPSLNGTL
                     RLYHAMDGIPIVVPEPFAEFARVPELGS"
     gene            complement(5380..>6189)
                     /gene="prfB"
                     /locus_tag="VNN25_22095"
     CDS             complement(5380..>6189)
                     /gene="prfB"
                     /locus_tag="VNN25_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXH94284.1"
                     /translation="MDALKTVVETLETKTLLSGENDGRNAIVTIHPGAGGTESQDWAE
                     MLLRMYLRYAERKGWSADIVDYQSGEEAGLKSATVMIRGDYAYGYMKSEHGVHRLVRI
                     SPFDAAKRRHTSFASVYVYPDIDEEIEIEINDKDLRVDTYRSSGAGGQHVNVTDSAVR
                     ITHIPTNIVVTCQNERSQHKNRDVAMKLLRSRLYQLEVEKRQEAQEKLEGEKKDIAFG
                     SQIRSYVMQPYQMIKDLRTGYEVGDVQRVMDGDLDGFVEAYLAQRARTVAD"
BASE COUNT         1206 a         1907 c         1912 g         1164 t
ORIGIN      
        1 tcgcgcccga cacaatctaa ttacgcccgg tacgtttcca acgccctacg cagcagctcg
       61 attgacgttc gcagatgctc ttcgttgagc acgtacgcga tccggatctc gcgcttgccg
      121 aggcctgacg acgcgtagaa cccgctggcg ggcgagacca tcaccgtgcc gccttcgtgc
      181 tcgaaatcgg tgagcagcca gaccgcgaag tcttcggcat catcgaccgg cagccgcggc
      241 acgcagtaga acgctccctc cggcttctcc agttcgacgc cgggaatcgc atgaagtccg
      301 ttgtagagca catcacgccg gcgctcgtac tcagccacga catcgcgcgt gtagtcatcg
      361 cccaaaccct gtgcaccgac ggcgatgaac tgcgccaggc cgggaggcga caacctcccc
      421 tgcgccatcc tcaaacaagc gtcataaacc tcgccgttgc gggtgacgag cgcgccgagt
      481 cggatgccgc aggcgctgta acgcttggag agcgagtcga cgacgacgac aaactccgag
      541 gcgtcccgca gctcaagagc gctgatcgcc ttgcggccgt catagacgaa ctcgcggtag
      601 acctcgtcgg agatgaggaa caagccgtgg tcacggcaga agctggccag catctccaat
      661 tcgtcgcgcg tgtagaccgt gccggtcgga ttgctcggat tgcagaggac gacgatgcgc
      721 gtccgcggcg tcagcgcgcg ctcgaacatc gcgcgcgaag ggagatgaaa tccatcccgt
      781 ccgcggctcg tcaccggcac gatgttcagt ccagccatcg tcgtgaaggc acgatagttc
      841 gcataaaacg gctcgatcag cagcacgtca tcgccttcgt tggcgcaagc caggaacgcg
      901 aagaggaggg cctcgctgcc cccggtcgta gccaggatct ggcgcgtctc gatcgggatg
      961 ccgaggcggc gctggtagta gtggcgaaga gaatcgagga acgcgggcgt gccggttgac
     1021 ggcgaatact ggaggacctt gtcttcgatc tgcttgagcc gctccatcat gcacgccggc
     1081 gtctcgatat cgggctggcc gatgttgagg tgatagacct tcgtgccacg cgatgccgct
     1141 agctcagcaa gcggcgcaag cttccggatc ggggaagcag gcatggcctg cccgcgccgc
     1201 gagatcgcga aggtgcttac cgtgtcttcg ggggcgacgg tcatggcgcg cttcaactgc
     1261 gaacgatgtg ccgttattgc ggctgcgata ccgcgatgga atcagcccac ggtctcctcc
     1321 gtttcgcgac gcatgaaacg actcgccttc gcgcttctcg tgctgtctct gacctcggcc
     1381 acgtttgctg ccgacgagcc tgaaaaacct aaggcaccgg ccgagaagcc ggccccgaac
     1441 ctcggcgcac gcgccgacaa gctcatcaaa gacgctctgc cagtctgcgc ggaagagatg
     1501 aaggagtctc gcgtcgctct cacgcacaag cttccggaaa acatgatcgg cgccgtgatc
     1561 cgcgaggaga gcaagcattc gccgtgtgaa ggacaatggg tcgcggcgat ctccaacgaa
     1621 ggtggcttct tcatgggcac gccctggttc ctggatgaca ccgaggggac gctcgaggtg
     1681 cggctgaagt cgttcactct tcaatacatg aagcaggttt tcgaaccgac gatcgatcgg
     1741 acgaagacac gcgaaggtct cttccacgtc acgctcacgc aggcagtcga cggcggaggc
     1801 aaattgccac tcgagggcga gatcgatccg gccggcacgg tccttttcat cggccacttc
     1861 gttccgatga gcgtggatta ccacacctcg cgcctcaaag ccctcgatca cttcctcagt
     1921 ctctcgccga caacaggctc cgcgaagcct gacgtgaccg tgatcgagtt ctccgatttc
     1981 gagtgcccgt cgtgccagca tgctgccgga tatctcaaac cgatcctgac cgcgcacggc
     2041 gaccgcgtcc gctacatccg ctacgacaca ccgctcgtga gcattcatcc ctgggcgttg
     2101 cccgcagcca tcgccggccg cgcgatctgg catcagaagc cggacctctt ctggaagtac
     2161 aaagagcagg tctacgagaa ccaggagaag ctctccgcgt tcacgatcga cgatttcacc
     2221 cgcggcttcg cctcggacca cgatctcgac atgaagaagt acgacgccga cgtcaactcg
     2281 cccgagcttc gcaccgagat catcaacggc gccggcaacg cattcacaaa cgacgtccgc
     2341 gccacgccga cgtacatcgt caacggcgcg ttcgtcgact ccggggtcga tggaaaaggg
     2401 ctcgtggatt acgtgaacgg gctgctgaaa aagtaattgg ccattcggcc tgccgattca
     2461 tggacgtcga tgcacaagtc gaaatcgacc ggctgacgga ttcattcttc gcgctctact
     2521 ccaaccgcga tggcgtgcgg cctgatctcg gcaggatctt cgacctcttc gttcccaatg
     2581 gcctgatcgc gaaatgcacc ggccccgatc cggagatctc aacccttcac gagttcattg
     2641 cccctcggca ggagttgctg accaacggca gccttacgga cttttcggag tctgagacct
     2701 ccaagcacac tcagatcttc ggcaacattg cccagcgcat cagtacgtat caaaagagcg
     2761 gctgtctcga tggcgtcgcg ttcacggcac gaggtgtcaa gacgtttcaa tttctttgga
     2821 cgcgaacggg ttggcggatt ctgtccgtga gctgggacga tgagcgcgaa ggattggccg
     2881 ttgaggagtt gtcgtagttg gactggcaag cgacctccaa taccgtcgcg aaataggtgt
     2941 catcctgagg ctgccggagg cgcccgcacc gcttgcggct ctcgctctga acgttcgagg
     3001 cgccggagga cgacgaagga tctcaaaatg cgatgcatgt ggcgtgcgca tcgtaatttg
     3061 agatccttcg ccgtcctacg ccgccacttc acgttgcgcg aaggctcgaa cacgaatggc
     3121 ggctccggct gtctcaggat gacactaata atgattgcga atgccggggc agggctcgac
     3181 agtgactctg tcacaccgcg cgccacacga aatcgagaaa gtgcttgagt atccggcctg
     3241 tcaatcccca gatctcgtac tgttcatagc gatagaaata ggccggatac tgtttgttgc
     3301 ggaacgggac gtaacgcact tcaggttgtt gtggcgatag aagcgcttcg atcggcactt
     3361 cgaacacctc gtcgacttcg gaagcctgca aaacgtaatc gattcgctga tcgatcacgc
     3421 ccacgaacgg cgcgacgaga aagccgctgt gggtgacgag atcgtcgagc ctgccgatca
     3481 cttccacacg ctcggtagcg atgccgactt cttcatgcgc ctcccgaatc gccgcagctt
     3541 cgagcgcttc gccggactcc actccgcctc cgggaaacga aatctgcccg ctatgtgcag
     3601 cgaggttgat cgagcgaagc gagaagagaa tgcaccagcc atgatcgtgc ggcacaagag
     3661 ggatcagaac cgcggcgcgc ttcaagtgcg gtgcttcgat ctcgattgcg ggtcgcgtac
     3721 tcaggatcgt gcgcaggcga gctaatgccg cgatgtcgaa tcccgggggt cgggtgtcgg
     3781 gagtcggggg tcggagatca ggcattattg gatgcgctca tggcgcgttt cttaagaacc
     3841 caactccggc acccgcgcaa actccgcaaa cggctctgga accacgatcg gaatcccgtc
     3901 catggcgtga tagagccgta gcgttccgtt gagcgaaggg agatcgactt cggcgatatc
     3961 gcccggacga agatcgcgcg gtaattcgat ccagcggtcg tcgactactt tgtcgccctc
     4021 gaacgtgagc atcatcaatc gatagccggg ctggccgtag ctgtgcgttc gcagagtgaa
     4081 ttcgccggca ttacgcaacg cgagcttttc gccgcgcagc ccggcttcga tcttcggaaa
     4141 gagcggcaac gtgcgggtga ttgcggtgcg cgtgtcgcgt gatcgatcgt tcaacgcggc
     4201 gagatatccc tgcaccgtca gatccatcga cgcgttctcg gcgagccagg cagtttgtgc
     4261 cttcgaagga tcggggatct cgcggacgaa aaagccggcg agcgtcgtcg cttccctgtc
     4321 gccgaacgga atcttcacga cgcaatcgtc gggaagctcg gcgaactgcg catagtcgct
     4381 gaccgcgacc ggcctgccgg cctcgaacgc gcgaatcagt gttcccgacg tctcgccggc
     4441 actcggatag cgcaggttga cgagacggtc gacggcggca aagtaggcag cgaattcgtc
     4501 gtcgggaacg tagccggtga aggtgatgcc gtcgccgcga agcgagtcga catcgacgtt
     4561 cggcgcgggc tcgccgacga caagcaaccg cagtcgcggt tgctgcgcac gcgcctgggc
     4621 gaaggccgcg agcacgacgt cggcgcgctt ggccgaggta aggaagccga agagtccgat
     4681 gacgcggttg ccggatgaga aggcgtgctg cgcgcgcacc gcgtcacgct gtccgcgcgc
     4741 ggacggttgc ggctcgaacg gatgcggcac gaggtggatc ggcgccgtca cgccgaaaga
     4801 acgaaggcgg tccgcggcgt aatggttgtg cacgatgacc gcgagcgaac gattcgcaac
     4861 atcgaccgat gcaggcatga ggaagttccc catctcgctg tgcagtcccg ccgcccgtcc
     4921 gcgcgcccat gcggcgcctg cttctccgtg attcgcggcg agcgccgcaa cgtagccatc
     4981 gatatcgccg cgcgcgagcg tcatctcgac gatgaggtgg tgcaacacca gatcgtgcag
     5041 cacgatcacg ccgggtgttc gcatcgcttc ttcgtagatc catgcgtggt gggggttgtt
     5101 gccgaggaag taaatgcggg tgtcgggagt cgggggtcgg gggtcggaga gaggctccga
     5161 gatcgtgacc acttcgatct tctcgcgcag cgcgggcagc agcatcgccg cgtagtgcgc
     5221 gacgccggtg cgcgtcggag ggagcggcga atacaaagcg atcttcattt tctgcctact
     5281 gcgcggaagc gtcagaagca ttcaccacag aggcacagag gacacagagg ttttctctgt
     5341 gacctctgtg tctctgtggt gatccgcttt caagacgcat caatccgcca ccgtcctcgc
     5401 cctctgcgca agatacgcct cgacgaagcc gtcgagatcg ccgtccatca cccgctgcac
     5461 gtcgccgact tcgtagccgg tgcgcaggtc cttgatcatc tgatacggct gcatgacgta
     5521 cgagcggatc tgcgagccga aggcaatgtc cttcttctcc ccttcaagtt tctcctgcgc
     5581 ctcctgccgc ttctcgactt cgagctgata gagccgcgag cggaggagct tcatcgccac
     5641 gtcgcgattc ttgtgctggc tgcgctcgtt ctggcaggtg acgacaatgt tggttgggat
     5701 atgcgtgatg cgcacggccg agtcggtgac gttgacgtgc tgcccgccgg cgccgctcga
     5761 tcggtacgta tcgacgcgca gatccttgtc gttgatctcg atctcgatct cttcgtcgat
     5821 gtcgggatag acgtagacgg aggcaaacga cgtgtggcgg cgcttggcgg cgtcgaatgg
     5881 cgagatacgc acgaggcgat gaacgccatg ctccgatttc atgtagccgt acgcgtagtc
     5941 gccgcggatc atcaccgtcg cgctcttgag gcccgcctct tcgccggact ggtaatcgac
     6001 gatgtccgcc gaccacccct tgcgctcggc gtagcgaagg tacatgcgca gcagcatctc
     6061 cgcccaatcc tgcgactcgg tgccgcccgc gcctggatga atggtgacga tggcgttgcg
     6121 gccatcgttc tcaccggaaa gcagggtctt ggtctcgagt gtctcgacga cggtcttgag
     6181 cgcatccat
//
LOCUS       DATLVW010000754         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_484946, whole genome shotgun sequence.
ACCESSION   DATLVW010000754 DATLVW010000000
VERSION     DATLVW010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484946"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VNN25_22100"
     CDS             <1..1938
                     /locus_tag="VNN25_22100"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH94285.1"
                     /translation="KIVLGKQKEDKTGSRYPAGLAVSKNGRYLYVVENVGDALAVVDL
                     ATSDIERLPTDHYPYAVAVASDGNVYVSNWGADTISIFHARTNGTLFAKSRLHVGRHP
                     SALVTNESGSRLFVALAGSDRIAVIDTHSRKIVRMLSDRAPGGPSEGSTPNALALDET
                     RLYVAEGDNNAVAVFDLDTLKLIGRIPTDWYPTAVASVGHDLLMLNGKGHGSHANPTG
                     PTPGLGIQRPLGYALGQLNGTLRITRKNPSLIDYSRRVAAANNWNMTRSPRHYPPFKH
                     VIYIIKENRTYDQIFGDLKEGDGDPSLVFFDKTSTPNHRALALRFGLFDRFFTNAEVS
                     SQGHIWSTAAYVTDFGEKTIPSGYSDRRGAIDGEEIDEPENGFLWTMAKKAGVSFRDY
                     GEMVNGPQGWPKTQADLGSDISPAYSPFNVEIPDQKRADAWIEEHRRFVTDANMPALE
                     VMHLPSDHTAGGNPKYHSPRAYMADNDLALGRIVEELTKSPYWRDTVVFVLEDDSQAG
                     PDHVDSHRGPLLVISAYNRPGTIHRFANTTDVVAAIEDILGLDRLSKFDYFSRSLADV
                     FTTTPNLAPYIAITPEAPLDEMNPPKTAAARMSEGLDFRRPDRIDDAFFNQLLWLMIK
                     GDKPMPAAQSRASLHLLQISK"
BASE COUNT          419 a          672 c          580 g          364 t
ORIGIN      
        1 aagatcgtcc tcggcaagca gaaagaggac aagaccggct cgcgctatcc ggccggtctg
       61 gccgtgtcga agaatggacg ctatctctac gtcgtcgaaa acgtcggcga cgcgctcgcg
      121 gtcgtcgatc tggcgacttc cgatatcgaa cggcttccca ctgatcatta cccgtacgcc
      181 gtcgcagtgg cttcggacgg caacgtgtac gtttcgaact ggggcgccga cacgatctcg
      241 atctttcacg cacgcaccaa cggaacgctc ttcgctaagt cacgtctgca cgtggggcgt
      301 catccatcgg cgctcgtgac gaacgagtcg ggatcgcggc ttttcgtcgc gctggcgggc
      361 agcgatcgca tcgctgtcat cgacacgcac tcgcgaaaaa tcgttcgcat gctgagcgac
      421 cgcgcgccgg gcggaccttc cgaggggagc acgccgaatg cgcttgcatt ggatgaaacg
      481 agactctatg tcgcggaggg cgacaacaac gccgtcgccg tcttcgatct cgatacgctg
      541 aaactgatcg ggcgaatccc taccgactgg tatccaacgg ccgtcgccag cgtcggccac
      601 gacctgctca tgctgaatgg caaaggtcat ggcagccacg ccaatcccac cgggccgacg
      661 cccggtctgg ggattcagcg tccgctcggt tacgcgctgg gacagctcaa cgggacgcta
      721 cgcatcacca ggaagaaccc atcgctgatc gactactcgc gccgtgtcgc cgcggcgaac
      781 aactggaaca tgacgcgttc gccgcgccac tacccgccct tcaagcacgt catctacatc
      841 atcaaagaga accgtaccta cgatcagatc ttcggcgatc tgaaagaggg cgacggcgat
      901 ccgagcctcg tcttcttcga caagacctcg acaccaaatc atcgcgcgct ggcccttcgc
      961 ttcggactct tcgaccgctt ctttaccaac gccgaggtca gctcgcaggg gcatatctgg
     1021 tcgaccgcgg cgtacgtcac ggacttcggc gagaagacga ttccctcggg ttactccgat
     1081 cgccgcggtg ccatcgatgg cgaggagatc gacgagccag agaacggttt tctctggacg
     1141 atggctaaga aagccggcgt gtcgttccgc gattacggcg agatggtgaa cgggccgcag
     1201 ggctggccga agacgcaggc ggacctcggc agcgacatca gccctgccta ttcgccattc
     1261 aacgtcgaga tccccgacca gaaacgcgcc gacgcctgga tcgaagagca tcgccgcttc
     1321 gtcaccgacg ccaatatgcc cgcgctcgaa gtcatgcacc tcccctccga tcacactgcg
     1381 ggcggcaatc cgaagtacca ctcgccgcgc gcatacatgg ccgacaacga cctcgcactc
     1441 ggccgcatcg tggaggagtt gacgaagtca ccgtactggc gcgacaccgt cgtattcgtg
     1501 ctcgaggacg attcgcaggc cggccccgat cacgtcgatt cccatcgcgg accgctgctg
     1561 gtcatctccg cctacaaccg gcctggaacg attcaccgtt ttgcgaatac gaccgacgtc
     1621 gtcgccgcga tcgaagacat cctcggactc gatcgtctgt cgaagttcga ctacttcagc
     1681 cgctccctgg ccgatgtctt cacgacaact ccaaacctcg cgccgtacat cgccatcacg
     1741 ccggaagcgc cgctcgatga gatgaatcct cccaagaccg ccgcggccag gatgtcggag
     1801 ggtctggact ttcgcagacc tgaccgaatc gacgatgcgt ttttcaatca gcttctctgg
     1861 ctgatgatca agggcgacaa gccgatgccg gccgcccagt cacgggcatc actacacctg
     1921 ctgcagatca gtaagtgagg cgcagacagg gcaacggtca ggtcggttta cgactttgtg
     1981 cgtaccgcta cgaggccgat gaccatcatc agcgcgcaga gtgccatgag accca
//
LOCUS       DATLVW010000755         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_280853, whole genome shotgun sequence.
ACCESSION   DATLVW010000755 DATLVW010000000
VERSION     DATLVW010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280853"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VNN25_22105"
     CDS             complement(<1..314)
                     /locus_tag="VNN25_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94286.1"
                     /translation="MADKKTDGEALHTFHPVLTDEQFQKAQQKARERVEKERVEAAMK
                     EIEDKEYGKAQRAAGMSKSDLKDDMVSITIDLVDPDINSCLVVNFKQWWHGETYHNVP
                     RHV"
     gene            complement(330..905)
                     /locus_tag="VNN25_22110"
     CDS             complement(330..905)
                     /locus_tag="VNN25_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94287.1"
                     /translation="MAVKTLGTIGTTTLSAVQWPSPELAVPVSEADLATIAQSILDDR
                     AGVDPLSGTYPASAGGMKLADRIWPGAFSRNMILDVPNRGQLRILPGDWIAVDLNGAV
                     ILIPERSLPNTLTATGDTHTNTTLDNLSVNVFTLGWTVGLRIVDTTNAGIPAATRISA
                     ISADGKTITLSKAATATNAGGTMTVGDFTHS"
     gene            complement(921..1385)
                     /locus_tag="VNN25_22115"
     CDS             complement(921..1385)
                     /locus_tag="VNN25_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94288.1"
                     /translation="MQTFHSPKKSDAGIASVAEKASDFFRAVAVVDMKTTFASGFVGL
                     ADRALSILLDQHGVVAAERHPVNGLQKIVRIPALVSPAPRSCASGVLFGKGKAMLSAG
                     FARAHSAICGYFAGGSLVEFRKELFGLAVRTKLVSADEFWSDHGLVISNPRS"
     gene            complement(1435..2796)
                     /locus_tag="VNN25_22120"
     CDS             complement(1435..2796)
                     /locus_tag="VNN25_22120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HXH94289.1"
                     /translation="MAGINVASQYSGDLVNYIAKKTLPLTRKQLVAYQFGDPLTLPKG
                     MGSTYSATRYNRLPLPQYPISEGVSPDGETMTISTVTAVAQQWADSVKITDVAEMTIF
                     HPPFQKAIELIGLQAAETFERNTMNALMAGTQVNFVNSRGSRAALTAGDVLDSTTVIR
                     TDAALETLGAPRFMGDEQTDTKISAESGGAKASSNPRAMPHYAAIIHTLVVGDFRQNS
                     TVVLAWTYSDINRLYNYEAGEWSGIRFCKSNMVPSFTGVANNDSGLAYAVNIGNGALP
                     ANTGYRIIVTGSDNQNQYESRIYQVSGAQNVALNGSITVTTPSTAGFTYSVYIGTSTS
                     PTNLGVTTAGPTTGPMQGMAVQLPPATAVVITAVGNAQTPPAAPATGVTVFPTFIIGR
                     GAYGQVMLDNITYTYLKDADKSDVHNLLRVVGWKAMYGTLIQNQQFMARIESSSAFTA
                     SFG"
     gene            complement(2841..>3146)
                     /locus_tag="VNN25_22125"
     CDS             complement(2841..>3146)
                     /locus_tag="VNN25_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94290.1"
                     /translation="DLCRDSPALAKIQPDVEDSIRQMRAQGNYQATREAVAKYILGDR
                     ALKRAAGNGGKQRQRGAEQVRRQTVRTGQGGSDVSANRRRGGDDLASLERRLAKQII"
BASE COUNT          533 a          922 c         1048 g          643 t
ORIGIN      
        1 acgtggcgcg gaacgttgtg ataggtctcg ccgtgccacc actgtttgaa gttgacgacc
       61 aagcacgagt tgatgtcggg gtcgacgaga tcgatggtga tcgacaccat gtcgtccttc
      121 aggtccgact tcgacatgcc cgccgcgcgt tgcgccttgc cgtattcctt atcctcgatc
      181 tccttcatcg cagcctcgac gcgctccttc tcgacgcgct cacgcgcctt ctgctgcgcc
      241 ttctggaact gctcgtcggt gagaacggga tggaaggtgt gcaacgcctc gccatcagtc
      301 tttttgtcag ccatatcaac ctcttaggtc tatgagtgcg taaaatcgcc gaccgtcatg
      361 gtgccgcccg cgttggtggc ggtcgccgcc ttggacagcg tgatcgtctt gccgtcggcg
      421 gagatcgccg agatgcgcgt cgccgccggg atgccggcgt tggtcgtgtc gacgatgcgc
      481 aaccccacgg tccagccgag ggtaaagacg ttgaccgaaa ggttgtcgag cgtcgtgttg
      541 gtgtgggtgt cgccggtcgc cgtcagcgtg ttcggcagcg agcgctccgg tatcagaatg
      601 acggcgccat tgagatcgac cgcgatccag tcgccgggca ggatgcgcaa ctgcccccgg
      661 ttcggcacgt cgaggatcat gttgcgcgag aaggcgcccg gccagatgcg gtcggccagc
      721 ttcatgccgc cagcactcgc cgggtaggtg cctgagagcg ggtcgacacc cgcccgatca
      781 tcgaggatgc tctgcgcgat ggtcgcgagg tcggcctcgg agaccggcac cgccaactct
      841 ggcgatggcc attgaactgc tgagagcgtc gtcgttccga tggtacctaa cgtcttgacg
      901 gccatgaggt tatccttgca tcaagagcgt gggttagata taactaggcc atggtcagac
      961 caaaactcgt ccgcactcac aagttttgtc ctcactgcaa gaccgaaaag ctccttgcgg
     1021 aattctacaa gagagccacc ggcaaaatat ccgcatattg ccgagtgtgc tctcgcaaaa
     1081 cccgccgaga gcatcgcctt gcctttcccg aaaagcacgc cgctcgcgca agatcgtggc
     1141 gccggcgaaa ccagggcagg aatgcggact attttttgaa gtccatttac gggatgccgc
     1201 tcggcggcta cgacgccatg ttggtcaagc aggatggaaa gtgcgcgatc tgccaaaccg
     1261 acaaacccgg aggcaaaggt cgttttcatg tcgaccactg ccacggcacg aaagaagtcc
     1321 gaggccttct ctgccacaga tgcaataccg gcatcggact tcttgggact gtggaaagtt
     1381 tgcaaagcgc catttcctat ttgaacaagt tttctcctgc taactcattg ataactaacc
     1441 gaaagacgcc gtgaaggcag acgagctttc tattctggcc ataaattgtt ggttctggat
     1501 cagagtcccg tacatcgcct tccagccgac aacccgcagc aggttgtgga cgtccgactt
     1561 atcggcgtcc ttcaggtagg tgtacgtgat attgtcgagc atcacctgac cgtaggcgcc
     1621 gcgtccgatg atgaaggtgg ggaacacggt gacgccggtc gccggggcgg ccgggggcgt
     1681 ctgcgcgttg ccgacggcgg tgatgacgac ggcggtcgcc gggggaagct ggacggccat
     1741 gccctgcatc ggtccggtcg tcggtccggc ggtggtgacg ccgaggttcg tcggcgaggt
     1801 cgaggtgccg atatagacgc tgtaggtgaa gccggcggtc gatggggtgg tgacggtgat
     1861 cgagccgttg agggcgacgt tctgcgcgcc ggagacctgg tagatgcggc tttcgtactg
     1921 gttctggttg tccgagccgg tgacgatgat gcggtagccg gtgttggcgg gcagcgcgcc
     1981 gttgccgata ttgaccgcgt aggcgaggcc agagtcgttg ttggcgacgc cggtgaagct
     2041 cggcaccatg ttcgacttgc agaaacggat gcccgaccac tcgcccgcct cgtagttgta
     2101 gagccggttg atgtcggaat aggtccaagc cagcaccacc gtcgagttct ggcggaagtc
     2161 gccgacgacg agagtgtgga tgatggcggc gtagtgcggc atggcgcgcg gattcgagct
     2221 cgccttggcg ccgccgctct cggcgctgat cttggtgtcg gtctgctcgt cgcccatgaa
     2281 gcgcggggcg cccagggtct cgagcgcggc gtcggtgcgg atgacggttg tcgagtcgag
     2341 gacatcgccc gcggtgaggg cggcgcgcga gcctctgctg ttgacgaagt tgacctgggt
     2401 tccggccatc agcgcgttca tcgtgttgcg ctcgaaggtc tccgccgctt gcaggccgat
     2461 cagctcgatg gccttctgga acggcgggtg gaagatcgtc atctcggcga catcggtgat
     2521 cttcacgctg tcggcccatt gctgggcgac cgccgtcacc gtcgagatcg tcatcgtctc
     2581 gccgtcgggg gagacgcctt cggagatcgg atattgcggc agcggcaggc ggttgtagcg
     2641 cgttgcgctg tacgtcgagc ccatgccttt ggggagggtg aggggatcgc cgaactggta
     2701 ggcgacgagc tgcttgcgcg tcagcggcag cgtcttcttg gcgatgtagt tgacgagatc
     2761 gccagaatat tgggaagcta cgttgatgcc ggccatgtcg ccctctccta gaatgaggag
     2821 agagcggaaa cgctacctcc tcagatgatc tgcttcgcca gccggcgctc caacgaggcg
     2881 aggtcgtccc cgccgcgccg ccggttcgcg cttacgtcgc tgccgccctg acccgtcctg
     2941 acggtctggc ggcgtacctg ctcggccccg cgctggcgct gcttgccgcc attgccggcg
     3001 gcgcgcttga gcgcacggtc gccgaggatg tattttgcca ccgcctcgcg ggtcgcctga
     3061 tagttgccct gggcgcgcat ctggcggatc gaatcctcga cgtcgggctg aatcttggcg
     3121 agcgccgggc tgtcgcggca gagatc
//
LOCUS       DATLVW010000756         3329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_610065, whole genome shotgun sequence.
ACCESSION   DATLVW010000756 DATLVW010000000
VERSION     DATLVW010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610065"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VNN25_22130"
     CDS             <1..385
                     /locus_tag="VNN25_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HXH94291.1"
                     /translation="DFLRFTRELIRLRWQYPALRGEGFRVIHVHDGNRVLAFQRWIPG
                     VGEDVMIVINLANYNKYNYEIGFPRGGPWREVFNSDVYDNWVNPTAAGNSGGVVASAA
                     PLHDLPFSAALTLPANGLLVFSQHS"
     gene            complement(990..1481)
                     /locus_tag="VNN25_22135"
     CDS             complement(990..1481)
                     /locus_tag="VNN25_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH94292.1"
                     /translation="MALFLCLMLAIPAVFAIDPGEIPIGGTLRDATLRGLNGPPRKLK
                     SFRGTPLIINVWASWCGPCRQEMASLERLAWRAQPQYFAIIGISTDDDTDRAKGLLNA
                     TNATISHFIDHDLEMENMLGASHLPLTVLVDANGRVLRRVYGAQEWDGADSIRLIDDT
                     FHK"
     gene            1718..>3329
                     /locus_tag="VNN25_22140"
     CDS             1718..>3329
                     /locus_tag="VNN25_22140"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HXH94293.1"
                     /translation="MHSQSLPLRTLWSLLLVVGIFALPRTFAASAIDDTPSGTLYLRR
                     SAEGTVAEALRLATRMQVQITGNVARVHVQQEFSNNGEDWLEGLYVFPLSKDSAVDEL
                     VMKVGERTLHGEIQEKAKAQATYEKARSEGRRASIVDQERPNLFTSAVANIAPHSSVT
                     IEIAYLETLPFRDGRYTLHLPLAITPRYTSGVPQDATVTLPAATAALMNATLGTSATP
                     ERVQAARQHVSIDIDLEPGFALGEVLSLHHVVETQSAPSGQQIHLQSDSVAADRDFEL
                     TWTQSAAQAVAGAAFAEKIGDETFALLMLSAPQQHDEAVPPREVTFIIDTSGSMQGPS
                     IEQARAALSMGIDRLKPFDRFNVIRFASDYTTLFSSPEPVTVKSRALADNFVANLHAD
                     GGTEMRAPLESALSSPPNPEFLQQIIFITDGSVGNEAELIRLIHDRIGGKRLFTVGIG
                     AAPNAYFLGEAAAAGRGSYTLIAERDQVGARMRDLFAKLERPALIDLKVQWPQQMGVD
                     LAEPLPLDLYSGDPVVVMARMPQMASGEV"
BASE COUNT          665 a          999 c          977 g          688 t
ORIGIN      
        1 cgacttcctg cgcttcacac gcgaactgat ccgattacgg tggcaatatc cggcgctgcg
       61 tggcgaagga ttccgggtca ttcacgtaca cgatgggaat cgcgtgctgg cgtttcaacg
      121 ctggatcccg ggggtcgggg aggacgtcat gatcgtgatc aatctcgcca actacaacaa
      181 atacaattac gagatcggct ttccgcgggg agggccatgg cgagaagtgt tcaatagcga
      241 tgtctatgac aactgggtga accccacggc agccggaaat agcggtggcg tcgtcgcgag
      301 cgccgcgccg ctgcatgatc tcccattttc ggccgcgctt acattgccgg ccaacggttt
      361 actcgtgttc tcgcaacaca gctgatgggg ttcaaaggag agaccaagca catctattcg
      421 gcgcaagacc agctgcagac gatcatagtc atgccgctcc cacgtacctc agcgtgtcat
      481 caatgcgtcc atgtcgtttt gcatgcgctg attgagttca tccgaccaag ctgcgccggg
      541 cttttggctc aacttgcttt gcggtcgggg cggcggtgaa gtcttgctct ctctcggcga
      601 tttgcgagcg gcaagtttca gtttaaggat gggacggcgg gacgaggagt tagagggatt
      661 ggatgggtgc atagaggggt agatggctcc ttcacttata cgattgggtt gcttgtatcg
      721 gcactgatta ggtcacctgc ggcatattag tagccggtca tgacacttat acgtcttcga
      781 ttcgatcttg tcgataagcc tggcctcacc gtggagtcat tttgcggctt ggctgcctag
      841 aaatggcaca atgcagctcc tcacttgata gggacgtcgc gttgcggagg gcttgacgga
      901 atggggaata cttccagcca gaggaagaac agcgcaacag gcgccctcat gactgcgctc
      961 aatttccttt ttttaatcgt gatcgagatt catttgtgaa aggtatcgtc gatcaatcgg
     1021 attgaatcgg ccccgtccca ctcttgtgct ccgtagactc tcctgaggac tcgtccgttg
     1081 gcgtcgacga gcacggtcaa cggcaggtgc gatgcgccca gcatgttctc catctcaaga
     1141 tcatggtcga tgaaatggct aatggtcgca ttcgtagcat tcaacaggcc tttcgcgcga
     1201 tccgtgtcat catccgtgga gatgccgatg attgcgaaat attgcggttg agcgcgccac
     1261 gcgagccgct ccaatgaagc catctcctgc ctgcacgggc cgcaccagct cgcccacaca
     1321 ttgatgatca gcggtgtgcc gcgaaacgac ttgagctttc ggggcggacc gttcagtccg
     1381 cgcagtgtcg catctcgtag tgtcccgcca atcggtatct cgccggggtc aattgcgaaa
     1441 actgccggta tcgcgagcat gagacacagg aagagtgcca cgcactggaa cgatcgcttg
     1501 gatgggtgct ccacagcgac atattgacgg cgccgcaagt cgaattgcaa accggctttt
     1561 ccccctgata gctggggttg gatggatcac gctcacgctc acgcgccgca acgactgcca
     1621 tatttctaca caattcatcc tcgccttgcc ataaagggcg tccgagcagg acacatctta
     1681 agcttgtaat ggctccatct tgtccaggga gcctcacatg cactctcaat cccttcccct
     1741 gcgcaccctt tggtcgcttc tgctggtagt cggcattttt gcgctacccc gaaccttcgc
     1801 ggccagcgca atcgacgata cgccgtccgg aactttgtac ttgcgtcgct cggcagaggg
     1861 gacggtggcc gaggcgctgc gtctcgcaac tcgcatgcaa gtgcagatca ccggcaacgt
     1921 ggcgcgtgtg cacgtgcagc aggaattctc gaataatggt gaggactggc ttgaagggct
     1981 gtatgtcttc cccctgtcca aggacagcgc tgttgatgag cttgtgatga aagtcggcga
     2041 gcgcacgctc cacggcgaga tccaagaaaa ggcaaaagcg caggcgacat acgaaaaagc
     2101 gcgttccgaa ggccgccgcg ccagcatcgt ggaccaagaa cggcccaacc ttttcacctc
     2161 ggcggtcgcc aatatcgcgc cgcattcgag cgttaccatc gaaatcgcct acctcgaaac
     2221 ccttcctttc cgcgacgggc gctacacgct gcacctgccg ctcgccatca caccgcgcta
     2281 tacatccggg gtaccccagg acgccacagt cacgctgccg gccgcaaccg ccgccctcat
     2341 gaacgcgacc ttggggacat ccgcaacgcc agaacgggta caggcggcgc gccagcacgt
     2401 gagtatcgac atcgacttgg agccggggtt cgcgctcggc gaagtcctaa gcttgcatca
     2461 cgtcgtcgag acgcaaagcg cgccctcggg tcaacagatt catttgcaga gcgattccgt
     2521 cgcggccgat cgcgacttcg aactcacgtg gactcagtca gccgcccaag cagtggcggg
     2581 tgcggcattc gctgaaaaga tcggcgatga aactttcgcg ctgctgatgc tctcggcgcc
     2641 acagcaacac gatgaggctg tgccaccccg ggaggtcact tttatcatcg acacatcggg
     2701 gtccatgcag ggcccctcga tcgagcaggc ccgcgccgct ctctccatgg ggatcgatcg
     2761 cctgaagccg ttcgatcgat tcaacgtcat ccggttcgcg agcgactaca cgacgctctt
     2821 ttcttcgccg gagccggtca ccgtgaagag ccgcgcgctc gccgacaact ttgtcgccaa
     2881 cctgcatgcc gacggcggca ccgagatgcg cgctcccctg gaaagcgcct tgtcgagtcc
     2941 gccgaatccg gagttcttgc agcagatcat cttcatcacg gatggcagcg ttggcaacga
     3001 ggccgagctt atcaggctga tccacgaccg aatcggcggc aagcggttgt tcaccgtcgg
     3061 catcggcgcg gcgcccaacg cgtatttcct gggcgaggca gccgcagcgg ggcgcggcag
     3121 ctacaccctc attgccgagc gggatcaggt gggcgcgcgc atgcgggatc tgttcgccaa
     3181 gctggagcgg cctgccctca tcgatctcaa ggtgcagtgg ccgcaacaaa tgggcgtcga
     3241 cctggcggag cccttgcccc tggatctcta cagcggcgat cccgtcgtgg tgatggcgcg
     3301 tatgccgcaa atggcgtcgg gcgaagtcg
//
LOCUS       DATLVW010000757         6467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_320779, whole genome shotgun sequence.
ACCESSION   DATLVW010000757 DATLVW010000000
VERSION     DATLVW010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320779"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..2209
                     /locus_tag="VNN25_22145"
     CDS             200..2209
                     /locus_tag="VNN25_22145"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH94294.1"
                     /translation="MILPARLFAANMVYEQGHHDESEREIEGLLAAVSPDFPAYRVLL
                     LKELSKCHIARADWGGAIGLLEDCISRFDGLGEASNASGMRRSLAFVYDRIGDPATAW
                     KYRLVALHGLGGRSSADLEKAVASVADAAMLRHQWRTALSFLSLEIDIATRIKDDLQL
                     AATPLIRAVVRDQLGDETGALADIAWARQTMGRTKDPAYHAYLRVAELRASAMLSVTP
                     PAEAESLLSEAIEYQATQSDQLKLPGLFLQRARARRKMGNVADAMADVKRGIAELERG
                     RESLPAGEARWGAFHAAEELFDEGIDLAISLNDAGAAFRFAESARARGLLDSYERSAV
                     LDFRRLPAGTVVVEYASLPSRLVIFTADRAGVRFTTVECGREKLTAEADGFGEAIRQT
                     GSVVRNARSSALYRRLIEPVAVQLLNATTVVFVPDKATSTVAFSALADSNGRYLIEQY
                     PIVIAASAAAFEAAVERRGDVTSPRSALVLAASEPGAGVGMLTYAGGEGRHVARAYQT
                     FTLLSDESVEFDALVKRAPLADIIHFAGHAIGDDRGIEPASIVLRDKGHERRVGVAEI
                     MKLRLRPASIVVLAGCSTARGERRAAEGVISVAHGFLSAGASSVVATLWPIDDQAASI
                     FFPRLHEKLAEGLSAAEALRAAQLESIRRGDVPASLWAAVQNIGS"
     gene            2243..3139
                     /locus_tag="VNN25_22150"
     CDS             2243..3139
                     /locus_tag="VNN25_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94295.1"
                     /translation="MYTVNFIGLNYFNACKVGERDVLVPNGTPGSGKDDDQIPEHFAS
                     LFIEEDLCANDDWWPEHKQVRPVRLEIKLGEFRTANVIEFRIPAKPDPYTEPAAVRFS
                     CRDRTLTNLNLDDGLPKLQTMGFVLDDRPDAIAKVPLPGGALEVFRFGGATLVRWLIS
                     DHDDPITITAHDGKATKRVVLKKSDSQLPAEIVFSNTVDLLAPLMSPAGNGKSQNMSV
                     LGGAMAGMNTDAAGSMHNHGAAGHFVLYAKLDKDRNEQKFESPKIPDAMKLKSALFSN
                     AYLSYLSSLDETPDPPCSGGCC"
     gene            3157..4173
                     /locus_tag="VNN25_22155"
     CDS             3157..4173
                     /locus_tag="VNN25_22155"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXH94296.1"
                     /translation="MNYNQAAKLPPPSHTRGPAPVPAPAPVPAPPPVEPYGRQDLVMG
                     TMRKLLEQRAGVARNLVEIDMQLEYLFYDLNSKKDDPQDVETYDGTLGVTREFVSDYE
                     RPVGQLQWTDLTGRFQADGEDQGNVSGQRWGSGALISDDLFLTAGHCFRQEFRSWKVP
                     ERNGIPISPAEIAPLMCVCFNFQIDGETGQTRPEVCYPVIELVECSPSPDDPHSTADY
                     AIVRLGRDEKSGTLPGKMFGNLHVAKTDLTVKNASLCIIQHPNRKEKKVEAGHLLEIR
                     DGRIAYNDIGTFGGASGAPILNGITGEVVGVHIKGGALPIGGFNSGTTIGAIRAKSKA
                     LASL"
     gene            4286..4951
                     /locus_tag="VNN25_22160"
     CDS             4286..4951
                     /locus_tag="VNN25_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94297.1"
                     /translation="MKTPDVRDSRGAERFVASNPIEGRFGRTDVTIWDLAESGLQMEH
                     AEPLKIATQGLVGFKLDEMTVTVPGIVIWSHLSKTADAHGRFLYRSGVRLEADIELFA
                     SAINRLAGSGRIRRDTASLERKRKKHVERDQELRSRPVIQMIQAPSGPSPDVVLLVLH
                     ARDRLRENANDAIKWYNRAKFSIPEDSFSHVHHREEVLAVWEYLERSIDIQTILDIFM
                     MKR"
     gene            5092..5814
                     /locus_tag="VNN25_22165"
     CDS             5092..5814
                     /locus_tag="VNN25_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HXH94298.1"
                     /translation="MRIASIVVVLFAVAVSLSAQPSGQYLRLQGYPQHGYIEIPHDVL
                     LDTPAMTVEAWVSVRDAHSPGCSSIAGKDYRTGWWLGLCGTTMRSYFNGTGSQRDGGV
                     IPDTPTTVWIHIAAVTDGTTRKHYINGNLVSQGAEFPRTVSTLPLRIGSDPNWDFMPE
                     GGIDDLRIWKVARTQDQIRQTMFAPYLLEANDVSGMFQGLEAWYRFEGNATDSWRTHH
                     GTIIGTGIGFAAAPAFRRHAAY"
     gene            5844..>6467
                     /locus_tag="VNN25_22170"
     CDS             5844..>6467
                     /locus_tag="VNN25_22170"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXH94299.1"
                     /translation="MTRFVAALAISLSLASPCLAADTLADALRIIQGKELVDLTHSFS
                     PTTPVWSGFGPATMSAACDSKTHSPFTIEHDGFRTTFYSMVGQYGTHVDPPAHFRSDG
                     MTMDQIPLKQMILPLVVFDITPMLPKDPNHALTIDDIKAWEKAHGTVPAGAFAALRTD
                     MSKEWTTNPEHFKRSPFPAWSLAAIKFLFDERKVTAIGHESLDTDTTD"
BASE COUNT         1277 a         1959 c         2017 g         1214 t
ORIGIN      
        1 ggctgcggtc caaggcgacc gaatgctcga acgagccgtc gctacgatcg acgcgtccag
       61 cggtacgaaa cgttcgttgc ttgcgtctgg gcattctgaa tattgcgacg gtctggatgc
      121 atatgcagcg aggcgtccgg gggacgccga accactactc cgccgcgccg cggaaagatt
      181 cgagacagcg cggagtccga tgatcctgcc ggcgcgtctc ttcgccgcaa acatggttta
      241 tgaacagggc caccacgacg agtcggagcg cgagattgag ggactcctcg cggccgtgtc
      301 cccggatttc ccggcgtacc gcgttctcct gctcaaggag ctcagtaagt gccacatcgc
      361 gcgagccgat tggggtggtg cgatcgggct cctcgaagat tgcatatcgc gcttcgacgg
      421 tctgggtgag gcctcaaacg cttccggtat gcggcgttct ctcgcctttg tatatgaccg
      481 gatcggcgac cctgccaccg cttggaagta tcgcctggtc gcgctgcacg gcctcggcgg
      541 acgctccagt gccgacctcg aaaaggcagt ggcatcggtc gcggacgcgg cgatgctccg
      601 tcatcaatgg cgcacagctt tgtcgttcct ctcactcgag atcgacatcg caacccgcat
      661 caaagacgat ctgcaactgg ccgcaacccc gctcatccgc gcagtggtcc gcgaccaatt
      721 gggcgatgag actggcgcgt tggccgacat cgcctgggca agacagacga tgggacggac
      781 gaaggatccc gcctatcacg cctacctgcg cgttgccgaa ctgcgtgcgt cggcgatgct
      841 ctcggttacc cctcccgccg aggccgaatc gctgctgagc gaagcgatcg agtatcaagc
      901 gacgcaaagc gatcagctca aacttccggg cctcttcctg cagcgagcgc gtgcgcgccg
      961 gaaaatgggg aatgttgccg atgcgatggc cgacgtcaag cgcggcatcg ccgaactgga
     1021 gcgcggtcgt gagtctctgc cggcggggga ggcacgctgg ggcgcatttc atgccgcgga
     1081 agagttgttc gatgaaggga tcgatctggc catctcgctc aatgacgccg gcgcagcctt
     1141 ccgatttgcg gaaagcgcca gagctcgggg cctgcttgat tcttacgaac gctctgcggt
     1201 tctcgacttc cgccggttgc ctgcgggaac tgtcgtcgtc gaatatgcct cgcttccctc
     1261 gcggctggtc atcttcacag cagacagggc aggagtgcgc tttacaaccg tggagtgcgg
     1321 gcgcgagaaa ctgacggccg aggccgacgg tttcggcgaa gccattcgcc agacaggatc
     1381 ggtcgttcgc aacgcgcggt catctgcgct gtatcggcgg ctcatcgaac ctgtggcagt
     1441 gcaactcctc aatgcgacaa ccgttgtttt cgttccggat aaggcaacgt cgacggtggc
     1501 gttcagtgcc ctggccgatt ccaatggccg gtacctgatc gagcagtacc cgatcgtgat
     1561 cgcagcgagt gcggcggcgt ttgaagcggc agttgagcgg agaggcgacg ttacgtcgcc
     1621 acggtcggcc ctggtgcttg cggcgtcgga gccgggggcc ggtgtcggaa tgcttacgta
     1681 tgcgggtggc gagggacgtc atgttgcacg cgcctatcag acgttcacgc tcctctctga
     1741 cgagtctgtg gagttcgatg cgctggtcaa acgcgcgcca ctggccgaca tcattcactt
     1801 tgccggacat gcgatcgggg atgatcgcgg tatcgagcct gcctcgatcg ttttgcgaga
     1861 caagggacac gaacggcggg tcggcgtcgc ggagatcatg aagttgcggc tccgccccgc
     1921 atcgatcgtc gttctggccg ggtgcagtac cgcgcgtggc gagcgccgtg ccgcggaggg
     1981 cgtgatcagc gtcgcgcacg gatttctttc ggccggcgcg tcatcggtgg tcgcgacgct
     2041 ctggccgatc gacgatcagg cggcgtcgat ctttttcccg cgcctgcacg aaaagctcgc
     2101 cgagggcctt tctgccgcgg aagccttgcg cgctgcgcaa ctcgagtcga ttcgccgcgg
     2161 ggacgttccc gcgtcattgt gggcggccgt gcagaacatt ggaagttgat ccgtctcaag
     2221 tactgagaaa ggagaagctt ctatgtatac ggtcaatttt atcggcctga attatttcaa
     2281 tgcctgcaag gtcggagaac gagacgtact ggtccccaat gggacgccag gcagcggcaa
     2341 ggacgacgac cagattccgg agcacttcgc cagcttgttc atcgaggagg acttgtgcgc
     2401 gaacgatgac tggtggccgg agcacaaaca ggtccggccg gtccggctgg agatcaaact
     2461 gggcgagttc cgcaccgcca atgtgatcga gtttcggata ccggccaagc ccgatcccta
     2521 cacggagccg gctgcggtcc gcttttcatg ccgggaccgg acgctcacga atctgaatct
     2581 cgacgacgga ctgccgaagc ttcagacgat gggctttgtt ctcgacgatc gtcccgacgc
     2641 gatcgcaaag gttcctcttc ctggaggagc gctcgaggtg ttccgcttcg gcggcgcgac
     2701 gctcgtgcgg tggctgatct cggatcacga cgatccgatc acgattacgg cgcacgacgg
     2761 caaggcaacc aagcgggtgg tcttgaagaa atccgatagt cagctgccgg cggaaatcgt
     2821 cttctccaat accgtcgatc tcctggctcc tctaatgagc cccgccggca acggaaagag
     2881 ccagaacatg agcgtcctcg gtggcgcaat ggccggaatg aacacggacg ccgccggcag
     2941 tatgcacaat cacggagcgg ccggacactt cgtcctttat gcaaaactcg ataaggaccg
     3001 gaacgagcag aaattcgaat cgcccaaaat ccccgacgcc atgaagctga aatcggcgct
     3061 cttcagtaac gcctatctct cttacctaag ctcgctggac gaaactcccg atccgccctg
     3121 ctcgggcgga tgttgctgag tcaaaaggag gcaaccatga attacaacca ggccgcaaaa
     3181 ctacccccac cttcacacac acgcggaccg gcgcctgtac ccgcgcccgc acctgtaccg
     3241 gcacctccgc ccgtcgagcc ctacggacga caggatctgg tgatgggcac aatgcgaaaa
     3301 ctgctcgagc agcgcgccgg cgtggcccgc aacctcgtcg agatcgacat gcagctcgag
     3361 tatctgttct acgacctcaa ttccaagaag gacgatccgc aggatgtaga gacatacgac
     3421 ggcacactcg gcgttacgcg tgagttcgtc agtgactatg agcgtcccgt cggacaattg
     3481 cagtggaccg acctgaccgg ccgtttccag gctgacggcg aggaccaggg aaacgtcagc
     3541 ggtcagagat ggggatcggg tgcgctcatc agcgacgatc ttttcctgac ggccggccac
     3601 tgtttccgcc aggagttccg ctcgtggaaa gtgccggagc ggaatggcat accgatctcg
     3661 cccgccgaga ttgcgcctct tatgtgcgtc tgtttcaact tccagatcga cggcgaaacc
     3721 ggccagacac gccccgaggt gtgctacccg gtgatcgagc tggtggagtg ttcgccttcg
     3781 cccgacgatc cccattccac cgccgattac gcgatcgtca gactgggcag ggacgagaag
     3841 agcgggacgt tgccaggcaa gatgttcgga aatcttcatg tcgcgaagac cgatctgacg
     3901 gttaagaacg cgagcctctg catcattcaa catccgaatc ggaaagagaa aaaggtggag
     3961 gcggggcatc tgttggaaat ccgcgacgga cggatcgcct acaacgatat cggcacgttc
     4021 ggcggcgcct cgggtgctcc gattctcaac gggatcacag gcgaggtcgt cggcgtccac
     4081 atcaagggcg gcgcgttgcc gatcggcggc ttcaactccg gaacgacgat cggtgcaatc
     4141 cgagcaaaat ccaaggcgtt ggcgagcctg tagaaaacca cccaatcgtt tggccatcgc
     4201 acgggacctt tgattccggg cgatggccga cgaaggcttt tccagaccgt tcgcccggtt
     4261 gtaagattac ggttcgagct cgtaaatgaa gacgccggat gtgcgggatt cacgcggagc
     4321 tgagcgcttt gtcgccagta atccgatcga agggcgcttt ggaaggacag acgtcaccat
     4381 ttgggacctt gccgagagcg gtctgcagat ggagcacgcc gagcctctga agatcgccac
     4441 gcaaggactg gttggattca agctcgatga gatgacggtg accgttcccg ggatcgtcat
     4501 ctggagtcac ctttccaaga ccgccgatgc gcacggccgg tttctctacc gatcgggcgt
     4561 acgtctggaa gcggacatcg agctctttgc gtcggccatc aaccggcttg ccggctcggg
     4621 gaggatccgg cgcgacaccg catccctcga gcgtaaacgg aagaaacatg tggagagaga
     4681 tcaggaactg cgctcgcggc cggtcattca gatgattcag gcgccgtcgg gcccgtcgcc
     4741 ggacgtggtt ctcctcgtcc tgcatgcgcg ggaccgcctg cgcgagaatg cgaacgacgc
     4801 aatcaagtgg tacaaccgag ccaaattctc gattcccgag gattcgttca gtcacgttca
     4861 ccatcgggaa gaagtgctgg cggtatggga gtacctcgag cgtagcatcg atattcagac
     4921 gatcctggac attttcatga tgaagcggtg aggtgacggg gagtggggtg tggggtatgg
     4981 aagatcagag atgggagatt agagattagc gatcagacga gtctctgatc ccttgtctct
     5041 gatctctgtt ttgatccacg actcacatac ggacaggtac aatcctggaa catgagaatc
     5101 gcctcgatcg tggttgtact tttcgcagtc gccgtctcgc tctctgcgca gccctcgggc
     5161 cagtacctcc ggttgcaggg ctatccgcag cacggctaca tcgagattcc gcacgacgtc
     5221 ctcctcgaca cgcccgcgat gacggtcgag gcctgggtct ccgttcgtga cgcccactcg
     5281 cccggctgct cctcgatcgc tggaaaggat tacaggaccg gttggtggct cggtctctgc
     5341 ggcacgacga tgcggagcta tttcaacggt accgggagcc agagggacgg cggcgtcatc
     5401 ccggacacgc caactaccgt ctggatccac atcgcggctg tgacggacgg gacgacacgc
     5461 aaacactaca tcaacggaaa tctcgtgtcc cagggagccg agtttcctcg gacggtgtcg
     5521 acacttccgc tccgcatcgg gagcgacccg aactgggact tcatgccgga gggcggcatc
     5581 gacgacctgc gcatctggaa ggtcgcgcgg acacaggacc agattcgcca gacgatgttc
     5641 gccccctacc tcctggaggc gaacgacgtc tccggaatgt tccaggggct cgaggcctgg
     5701 taccgattcg agggcaatgc cacggactcg tggcgcacgc accatggaac gatcatcgga
     5761 accggaatcg gtttcgccgc cgccccagcc ttcaggcgtc acgccgcgta ttgatggttc
     5821 gcgcgaggag aatgggttac gtcatgacac gattcgtcgc cgcgctcgca atcagcctca
     5881 gcctcgcttc cccctgcctg gccgcagaca cgctcgccga tgcgctcagg atcatccagg
     5941 ggaaggagct cgtcgatctc acgcacagct tcagcccgac gacgcccgtg tggagcggct
     6001 tcgggccggc gacgatgagc gccgcgtgcg attcgaaaac gcacagcccc tttacgatcg
     6061 agcatgacgg cttccggacg acgttctact cgatggtcgg gcagtacggc acgcacgtcg
     6121 atccgcctgc gcatttccgc agcgacggca tgacgatgga ccagattccg ctgaagcaga
     6181 tgatcctgcc gctcgtcgtg ttcgacatca cgccgatgct gccgaaggat ccgaatcacg
     6241 ccctgaccat cgacgacatc aaggcgtggg agaaagcgca cggcaccgtc ccggccggcg
     6301 cgttcgccgc gctccgcacg gacatgagca aggagtggac gacaaacccc gagcacttca
     6361 agcgctcgcc gtttcccgcg tggtcgcttg ccgcgatcaa gtttctgttc gacgagcgaa
     6421 aggtaacggc gatcgggcat gagtcactcg ataccgacac gacggac
//
LOCUS       DATLVW010000758         6721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_30983, whole genome shotgun sequence.
ACCESSION   DATLVW010000758 DATLVW010000000
VERSION     DATLVW010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30983"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1061
                     /gene="truB"
                     /locus_tag="VNN25_22175"
     CDS             132..1061
                     /gene="truB"
                     /locus_tag="VNN25_22175"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXH94300.1"
                     /translation="MQPPRARVDGLLLVDKKTGITSHDVVEKFRRRSHIKKVGHTGTL
                     DPLATGLLVLCVGKATRLQAYLMGMEKVYEGVIQFGWATDSYDAAGTPVGEPNEVNVE
                     HIDFTPLIAPFLGAIEQMPPQFSAKKVGGVRAYELARKGEVAALTPKAVTVYEFEIIS
                     VTGSTARFRVRSSAGTYVRSLAHELGEAAGVPAHLKELRRTAIGNFKIADALSYEALE
                     NAPPEQLMTTPHFQSLSDVELPLPRLRIDWSQQGKLMRGQPIIMTPPEAVRAGDLLAL
                     GNPEDQLVVIGEVVNILREGGPVEVRPKVVLAG"
     gene            1147..1749
                     /locus_tag="VNN25_22180"
     CDS             1147..1749
                     /locus_tag="VNN25_22180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXH94301.1"
                     /translation="MPRTERDFADQGKEPARRRFMIAGEHVILRAFERDDAERCYRWI
                     NDPNIVRTLKTRYPIAFQNEIEWLDRAMHDSNVERHFAIERRDDRSHIGNASIHDIDW
                     VSQTAWFGLFIGEPTAWNRGFGGDAIQTLVRFAFDEMNLGKLRINVFDYNDRAKHVLE
                     ALGFVQEGLLRRDFFREGSYHDIVILSKFRADHSSPGSTG"
     gene            1760..2158
                     /locus_tag="VNN25_22185"
     CDS             1760..2158
                     /locus_tag="VNN25_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXH94302.1"
                     /translation="MSERKIRVLIAKPGLDGHDRGAKIIARALRDAGMEVIYTGLRQT
                     PEQIVAAAVQEDVDAIGLSILSGAHNVLFPEIMRLLHEESAEAIVVFAGGIIPEKDIP
                     KMKAIGIREIFLPGTPTATAVDAIRRAVNV"
     gene            2202..2678
                     /locus_tag="VNN25_22190"
     CDS             2202..2678
                     /locus_tag="VNN25_22190"
                     /inference="COORDINATES: protein motif:HMM:NF022664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HXH94303.1"
                     /translation="MFGFVIIISLFSTAVFAAETFRCESKDNRRHLCSFDSFGRVEIL
                     RQVSIAGCIEGSTWGREGRHRVWVSDGCRADFVVRRERDEDRDDHRDRERRGRLVTCE
                     SDYDSRHRCTVDTEGGVRLSRQLSRTNCVLDRTWGFDDRGIWVKNGCRAEFWVGRR"
     gene            3529..3642
                     /locus_tag="VNN25_22195"
     CDS             3529..3642
                     /locus_tag="VNN25_22195"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH94304.1"
                     /translation="MANTLSPPVLTIPGLWNSGPLHWQTHWEARQPSFRRG"
     gene            4134..4586
                     /locus_tag="VNN25_22200"
     CDS             4134..4586
                     /locus_tag="VNN25_22200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94305.1"
                     /translation="MNSNIDDAAANVMSPAAGLRILVVDDNPDVARTFSEVLSAEGHF
                     VDIADTSGAALPLIETGVYAVVLTDMRMPDLDGPALYREVFARCPPLAKSFLFITGDS
                     FSNDARRFLSETGAVCLTKPCTLDEIESAIERIAVQRHGRAQERVTTS"
     gene            4652..5281
                     /locus_tag="VNN25_22205"
     CDS             4652..5281
                     /locus_tag="VNN25_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH94306.1"
                     /translation="MRIVIADDHAVVRKGLRQLIVEADDMTVVGEAASGDELLTVLRS
                     RPVDVVILDLSLGARSGLDLLKHIKSELPRLPVIILSMHPEDLFAVRALRAGASGYVE
                     KNSAPEDLLMAIRRVSRGGTYVSAQIGEKIAADIARGGAAVLPHERLSDRELEVLRLL
                     GSGMSVTAIAHALNLSVKTVSTHRTRVLAKTGLRTNADIVEYVISHGLR"
     gene            5286..>6721
                     /locus_tag="VNN25_22210"
     CDS             5286..>6721
                     /locus_tag="VNN25_22210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXH94307.1"
                     /translation="MAKVRFLAVALAFVSIAAVAQPAGRSARTIRVVVDNSWYEPYVI
                     YAEYAAAAAFLVIVALIGWNYALRRRILQRTAALVESEQRFRQIAENIREVFWLLDVD
                     GGKTLYVSPAYETVMGRTRESLYQDLKSFTAAIHPDDQRRVSEAMEGDRERGFEVEYR
                     VVKPDGSIRWIWDRGFPIRNRAGHVYRLAGIAEDITDRKLAADALKDADARVRLIIDT
                     IPAMAWSSDPDGTVDYLNQRWLDYAGLSLEQYVQDPMGPVHPEDVSRVIEKWLVSMAA
                     VEPYEDEMRLQGADREYRWFLIRSVPLCDDEGHLVKWYGTSTDIEDRKRAEEKLKRSE
                     RQLAEAQRISHVGHWERDIDGGEIICSEETYRIFGLPPQDAMRNFEQLIHPEDRPLHA
                     AAIARALRGEPFDVEYRVVRPDGEIRFVHSQGSAVEDPSGRAHRTFGAVQDITELKHA
                     EEKLKTTSEQLRTLSARLQSAREAEGIR"
BASE COUNT         1326 a         2021 c         2076 g         1298 t
ORIGIN      
        1 tcgaacgcga aatgaacggc gaagttgccg gtccagtgca tgactggcgt catcgtacgc
       61 tgccgacgcg ctcgctggaa tggcttattc gctgacgaag attcggtgga tcgactatcc
      121 ttcgccccct catgcaacca ccccgcgccc gggtcgacgg cttgcttctg gtcgacaaga
      181 aaacaggaat cacgtcgcac gacgtcgtcg agaagttccg gcggcgctcg cacatcaaga
      241 aagtcgggca cacaggcacg ctcgatcccc tcgccaccgg tctgctcgta ctctgcgtcg
      301 gaaaggccac gcggttgcag gcctatctga tggggatgga gaaggtgtac gagggcgtca
      361 tccagttcgg atgggcgact gattcgtacg atgccgcagg cacgcccgtc ggtgagccca
      421 acgaagtcaa cgtcgagcac atcgatttca ccccgctcat cgcccccttt ctcggcgcga
      481 tcgagcagat gccgccgcag ttctccgcga agaaggtcgg cggcgttcgc gcctacgagc
      541 tggcgcgcaa aggggaagtg gcggcgctga cgccgaaggc ggtcaccgtt tacgagttcg
      601 agatcatcag cgtcaccggc tcgacggcgc gtttccgcgt ccgctcgtcg gcaggcacgt
      661 acgttcgatc actcgcgcac gagttgggcg aggccgcggg tgtacctgca catctcaaag
      721 agctccgccg caccgccatc ggcaacttca agatcgcaga tgcactgtcg tacgaagcgc
      781 tcgaaaacgc gccgcccgag cagttgatga cgacgccgca tttccaatcg ctctcggacg
      841 tcgagctgcc gctgccgcgt ctgcgcatcg actggtcgca gcaggggaag ctgatgcgag
      901 ggcagccgat catcatgacg ccgcccgagg ccgtgcgagc cggcgatctg ctcgcgctgg
      961 gcaacccgga agaccagctc gtcgtcatcg gcgaggtggt caacatcctt cgcgaaggcg
     1021 gtcctgtcga ggtgaggccg aaggttgttt tagcaggttg agggttcgcg tttcgggggt
     1081 cgggagtcgg atctgcgtcc atctgcgtca tctgcggatc aaaccggtct catataatcc
     1141 aatcacatgc cacgaaccga acgcgatttt gcggaccagg ggaaagaacc ggcgcggcgg
     1201 cgcttcatga ttgccggtga gcacgtgatc ctgcgtgctt tcgagcgcga cgatgccgag
     1261 cgctgctacc gctggatcaa cgatccgaac atcgtccgaa cgctgaagac gcgttacccg
     1321 atcgcgttcc agaacgagat cgaatggctc gaccgggcca tgcacgacag caatgtcgag
     1381 cgtcatttcg ccatcgagcg gcgcgatgac cgttcgcaca tcggcaacgc ctcgattcac
     1441 gacatcgact gggtctcgca gaccgcctgg ttcgggctct tcatcggcga gccgacggcg
     1501 tggaatcgcg gcttcggcgg cgacgccatc cagactctcg tccgcttcgc cttcgatgag
     1561 atgaacctcg gcaagctgcg catcaacgtg ttcgactata acgaccgcgc caagcacgtt
     1621 ctggaagcgc tcggcttcgt gcaggagggc ctcctccgcc gcgacttctt ccgcgaaggc
     1681 agctaccacg acatcgtgat cctctcgaag ttccgggcgg accattcgtc gccgggctcc
     1741 acgggataga cttcggctaa tgagcgagcg aaagatacga gtactgattg ccaagcctgg
     1801 gctcgatggg cacgaccgcg gcgcgaaaat catcgcccgc gcgctgcgcg atgccggcat
     1861 ggaagtgatc tacaccggcc tgcggcagac accggagcag atcgtcgcgg cggcggtgca
     1921 ggaggatgtc gatgccatcg gcctctcgat cctgtccggc gcgcacaacg ttctgtttcc
     1981 cgagatcatg cgtctgctgc acgaagagag cgccgaagcg atcgtcgtct tcgccggagg
     2041 tatcattccg gagaaggaca ttccgaagat gaaagcgatc ggcatccgcg agatttttct
     2101 tccaggtacg ccaactgcga cggccgtcga cgccattcga cgcgccgtga atgtgtagtc
     2161 gtcaaatcaa tcttcggggg gagattttca tgagagcgaa attgttcggg tttgtcatca
     2221 tcatctcgtt attcagcacg gcggtctttg cggcggagac gttccgctgc gagtccaaag
     2281 acaaccggcg ccacctgtgc tcctttgatt cattcggccg cgtcgagatc ctgcgtcagg
     2341 tcagcatcgc cggctgcatc gagggcagca cctggggtcg cgaaggacgc caccgcgtgt
     2401 gggtgagcga tggctgccgc gccgacttcg tggtccgtcg cgagcgcgac gaggaccggg
     2461 atgaccatcg tgatcgcgaa cgtcgcggac gcctggtcac ctgtgagtct gattacgatt
     2521 ccagacaccg atgcacggtc gacacggaag gcggcgttcg cctttcccgt cagttgagcc
     2581 ggaccaattg cgtgctcgat cgtacgtggg gttttgacga ccgcggaatc tgggtcaaga
     2641 acggctgccg cgccgagttc tgggtcggcc gacgctgaac gcggatttct gaggagttgc
     2701 ggccggaggg cttcctctgc cgcaactctt acgtcatcgg cgccgggcga ggtcgcgcgc
     2761 tacgctgcgg cgacgtgatc cgctgcgggc tacccgtgtt gatgatatcg gccaggcgcc
     2821 cggcacacga gctcgcggcc tgttgccaat cgcacgcgcg agtcgagctc gcattttctc
     2881 tcacctgtag acagttgacg gcagcgcctt tccatcgagc gccttctccc cacgcttttt
     2941 gtgggggaga aggtggccgg gtatagaccg ggaacatggg taacagatcg gcccggggac
     3001 ataggtgata gtcgggaacc ggtcgagagg cgtcgaggag accgacgatg cttggaacct
     3061 cgaggccgcc cgcccaagga ccgggctatg gaagctgacg tcctatggaa agccgcgatg
     3121 aggccgcgcc ttcccacagg acttggaaaa cgccccccaa ccaccacccg cgtttcccac
     3181 atctcccaca gcccctaccg ccagagaaag agagagaagg gggaaccaag aaccacaacc
     3241 tggccaatca ggagtgttac ctatgtcccc ggtccgatgt gttacctatg ttccaggtcg
     3301 tacaccgaag gccggatgag ggggcgcttg actgtcgcga cctcggtcca caagtagcaa
     3361 gcgcactcgg ggatgcatta ccgggtcggg gccttcaacg tacttttgcg acgcccccct
     3421 catccgcctt cggcaccttc tccccccgga aaaacctcgg ggggacaagg cgctcgacgg
     3481 aaaagcagtc gcaagaatgc gtgagaaatg ccggctaaac tccgccgtat ggcgaacact
     3541 ctcagccctc ccgttctcac gattcccggc ctttggaatt ccggtccgtt gcactggcag
     3601 acgcattggg aagcgaggca accctcgttt cggcgcggct gagcggcccg tcaaggaatc
     3661 gtcagtttcg gttgccgatg gttatccaaa gctcgatttc attgaggaca aacgatttaa
     3721 ctgtgagcac gacttcctca atctcaggac gatcgttctc aactttcagg acgttcgtcc
     3781 tcaacttttt caggacgttc gtcctcaact ttcaggacgt ttgtcctcaa tttcaggacg
     3841 ttcgtcctca actttgacga ctttctcctc aacgtcgaga acaatttcct caccgttaac
     3901 caggccggtt gacggacgga attcagaatt actcctacaa ccgaatcaga caagttctct
     3961 cgcggcaggc acggcaccgg tgtatttgtc attccggcca tgcaccggtt tggcgttgca
     4021 ctgagaaggc ggttggtgag acggcgcttg ccgagcccaa agctcgtgga atcggagccg
     4081 gcgaaacgaa tgggtgcaca acgccgatcg ttaccttgcc gttcggatct tccgtgaatt
     4141 cgaatatcga tgatgccgca gcgaacgtca tgtcaccggc agcggggttg cgcattcttg
     4201 tggtcgatga caatcctgat gtggcgagga cgttctccga agtgctctcc gctgagggcc
     4261 acttcgtgga catcgccgac accagcggag ccgcgctgcc gctcatcgag acgggcgtat
     4321 atgccgtcgt gctgacggac atgaggatgc ccgatctcga cggtccggcg ttataccggg
     4381 aggtcttcgc acgctgccca cctctcgcaa agtcgttctt gttcattact ggtgattcct
     4441 tcagcaacga cgctagacga ttcctaagcg aaacgggcgc cgtctgcctg accaagccgt
     4501 gcacgctcga cgagatcgag agtgcgatcg agcgcatagc tgttcagagg cacggacgtg
     4561 cccaggagcg agtgacaaca tcgtgaccgt cggcatgtca ctgacaagta gtggcggcgg
     4621 ccctatattg ctcgccaggg tctccccaat gatgcgaatc gtgatcgccg atgatcatgc
     4681 ggttgtccgc aaaggactgc ggcagctcat cgttgaggcg gacgacatga cggtcgtcgg
     4741 cgaggctgcc tcgggcgacg agctgctgac cgtcctgcgc tcgcgccccg tcgacgtcgt
     4801 catcctcgac ctcagcctcg gcgcccgcag tgggctcgac ctcctcaagc acatcaagag
     4861 cgaattgcct cggctgcccg tcatcattct gagcatgcat ccggaggatc tcttcgcggt
     4921 gcgcgctctg cgtgccggcg catcgggcta tgtagaaaag aacagtgctc ccgaggatct
     4981 gctgatggcc attcgacgcg tgtcacgcgg gggcacgtat gtgagcgcgc agatcggcga
     5041 gaagattgct gccgacatcg caagaggagg cgcagccgtc ctgccacacg agcgcctttc
     5101 cgatcgcgaa ttggaggttc tccgcctcct cggatcgggc atgtcggtta ccgcgatcgc
     5161 tcacgcactg aacctgagcg tgaagaccgt aagcacgcat cgcacgcgcg ttctggccaa
     5221 gaccgggctt cgaacgaatg ccgacatcgt cgagtatgtg atttcccacg ggcttcgctg
     5281 agaccatggc aaaggtccgc tttctcgctg tagcgctggc gtttgtttcg atcgccgccg
     5341 tggcgcagcc cgccggccgg agcgcgcgaa ccatacgagt cgtggtggac aacagttggt
     5401 atgaaccgta cgtcatctac gctgagtacg cggccgcagc ggcgttcctg gtcatcgtgg
     5461 cgctgatcgg ctggaactac gcgctgcgaa gacggatcct gcagcggacc gcggcattag
     5521 tcgagagcga acaacgcttt cgtcagattg cggagaacat tcgtgaggtc ttttggcttc
     5581 tcgatgtcga tggcggcaag acgctttatg taagcccggc ttatgagact gtcatggggc
     5641 gtacccgcga gagtttgtat caagacctga agtctttcac ggccgcgatt caccccgacg
     5701 accaacgtcg cgtgtccgaa gcgatggagg gagatcgcga gcgcggcttc gaagtcgagt
     5761 atcgtgtggt aaaaccggat gggtccatcc gctggatatg ggatcgtggg ttccccatca
     5821 gaaatcgagc ggggcacgtc tatcgcctcg ccggaatcgc cgaagacatc acggaccgga
     5881 agctggccgc ggatgccctg aaagacgccg acgcccgtgt ccgcctcatc atcgacacga
     5941 ttccggccat ggcctggagc tctgaccctg acggaaccgt cgattacctc aatcaacgct
     6001 ggctggacta tgccgggctt tcattggagc agtatgttca agatccgatg ggtcctgttc
     6061 atcccgagga cgtttcgaga gtcattgaaa aatggctggt gagcatggcc gctgttgagc
     6121 cctacgagga tgaaatgcgc ttgcaagggg ctgatcgcga atatcgctgg tttctgatcc
     6181 gctcagtccc gctgtgcgac gatgaggggc accttgtcaa atggtacgga acgagtaccg
     6241 acatcgaaga ccgcaagcga gcggaggaga agctcaaaag gagcgagagg cagttggccg
     6301 aagcgcagcg gatctcgcac gtgggccatt gggagcgcga tatcgatggc ggcgaaatca
     6361 tctgttccga ggaaacctac agaatcttcg ggctgccgcc gcaggacgca atgcggaact
     6421 ttgaacagtt gatccacccg gaggatcggc cactgcacgc cgcggccatc gcccgggcgc
     6481 tgcgaggaga accctttgac gttgaatatc gcgtggttcg gccggatggc gagatcaggt
     6541 tcgttcacag ccagggcagc gcggtcgagg atccgtcggg acgggcgcac cgcaccttcg
     6601 gcgctgtgca ggatattacg gagctcaagc acgccgagga gaaactcaag acaacaagcg
     6661 aacaattgcg taccctctcc gcccgtcttc aatcggcgcg ggaagcggaa gggatccgga
     6721 t
//
LOCUS       DATLVW010000759         7899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_241446, whole genome shotgun sequence.
ACCESSION   DATLVW010000759 DATLVW010000000
VERSION     DATLVW010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241446"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VNN25_22215"
     CDS             complement(<1..1869)
                     /locus_tag="VNN25_22215"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HXH94308.1"
                     /translation="MRLPFSSLVRSVGMFLLAAGLCTTLSAATVSKLNDDVLSPLRDS
                     VGAKQIHLNNVPLYAHGTVKIDLEEFQVWAPGGKVIVDDGKSVTYLDPPPMRFFRGMV
                     NGDPESFAYFSVDGTTGAISALIATRDGKFAIDASRRRATPKPGRAIDGDFDYFLTAM
                     DASDETPMTGQTWSCGVEKLPALPRVNQRLLTATDAKNHQVIAQGISGTQAYAITVEV
                     ETDSELYINAGSNSTTLTNYVTNLTGAVSTIYNRDIHTNVVQQNLHIYTGGNGSDPWA
                     ATDANSGLNELGDYYHTNHLSLKRSAVVMLSGKATNSGVAWEGVVGEGDFSNSGDFGG
                     AYAWCGSIGDLGGQGIGSVPDPNATNNGTLYGMPTGGNNYWPLEEYAHELGHNLAGHH
                     THCVVITSGEAAGTNHPSQLFIDMCFTGEIDQDGLGNTSACATGAQYSSAPTEKGTIM
                     SYCHNVFQGQVPQSRYTLGQASEVSHHELDDYMLRAAGPLGPYGGTSNIVTAVGTFSM
                     STITASSSVPASSTGNTASVTPSNGTTVTYAWTITGGTITSATNTASITYTAGASGSV
                     VLRATAYNTKGNNGEGFPAGTVGVTDTKTVTIVTVSPPTNVVATALTSTSVQVTWTA"
     gene            complement(2285..2695)
                     /locus_tag="VNN25_22220"
     CDS             complement(2285..2695)
                     /locus_tag="VNN25_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94309.1"
                     /translation="MTRATLLLILFAIAMPLAADEPRPKPDYSNEAMFRFAHDFVEHR
                     SGILDFDLGNLGAIDVYKYGVHGRFNYLPFLAPLLGTRLVDVATVPNAFTLTNTRIAR
                     SMPYMSDDTLSPGAKRELKTVLKIIAKDKVAVKE"
     gene            complement(2747..3085)
                     /locus_tag="VNN25_22225"
     CDS             complement(2747..3085)
                     /locus_tag="VNN25_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94310.1"
                     /translation="MRTLSIAVLILASSAVYAQQSTPSAATPPPQPGPVLQATTPDDA
                     SARLDVEIGAIRMTTHSGRYTMAYLPFLAPLQGTAFRASMEMPNALALTNTTIPQKPY
                     VQRSAVIRKR"
     gene            complement(3164..4837)
                     /locus_tag="VNN25_22230"
     CDS             complement(3164..4837)
                     /locus_tag="VNN25_22230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH94311.1"
                     /translation="MRRLAIAALFALSALPSPAQQPAGESIEVRVVNIDVVVRDKAGK
                     PVTGLTKDDFEIFENNKKQEITNLYEVRPSAPAAAAKVTPQPSNAAAPAATEAPADAR
                     RRNIVMFVDNYSLAPFQRDKILKSLQKFIDDDVRPQDQVMLVLCTQQTKVITPFTSDR
                     KLLQQGIESIRQTASSGFNRQHSVEEIKRRVNEYIDAGKEGKRPWKDYYQMSLSLVES
                     DTEEVIFASKNTLASLGQTTTALAGMEGKNVLVFAGAHLPEHPGVEMYQWLYNAFLPY
                     MGNVTFTSEGILGKSGSMQHYSIEEAAKMASVSGVALYIIDAADTRDTSSAERSSATD
                     QTEQFLTFTNTAMAYQTLARISGGMALTNSQNFDVAFQTVADDLNSYYSLGFKPSSGA
                     DNARRNLVVKTKNAQYNVRTRETYATKSTEDEMSARVIANINNSDPRGTWKIELHPGT
                     PEQQGNEFRVPVEISIAPTITLLPQNADLAGGFTVYIVVGSGGRTSKVIKSPHPVKIP
                     AEVEEEFRMSPMTYKTVILMTRGENILSTGILDQTSNSSGFARATVTVP"
     gene            complement(5836..6219)
                     /locus_tag="VNN25_22235"
     CDS             complement(5836..6219)
                     /locus_tag="VNN25_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94312.1"
                     /translation="MQNVSYQDPNLLLLEEDFVVAEENFLLLEQMHLLLEGNFFLLEQ
                     NFVLPEQNHLLPEQNFFLLEGNFFLRVENFSLPEQIHFLLEQNHLLPEQNLFLPEQNL
                     FLPEQNFLIPEQNFLAEEQNLSRAG"
     gene            6471..7043
                     /locus_tag="VNN25_22240"
     CDS             6471..7043
                     /locus_tag="VNN25_22240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94313.1"
                     /translation="MSTTTPNPGTPEAGTPAPSPQTLDAQERIARLRAIADDFPDEAS
                     PKPLTKAERNLAARTSIVFVEKAALFAEAAPNIGVALSVDTAVLRDAVASELANGGVI
                     DETRTLISRIEDANTRKKVKAVKVARGLYRVAKSYVTTDLGHVVTPHVDEMKRALQRP
                     RAKKKTAPAEPPATTPAATTSPSDSTSQQK"
     gene            7368..7826
                     /locus_tag="VNN25_22245"
     CDS             7368..7826
                     /locus_tag="VNN25_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94314.1"
                     /translation="MSIYATLWILKFPALGDSYFGCEWETVIAQGVPGHIGTPTEGHG
                     YEAGDPYSSFLPPAIAISDDDDQATPLRAVVIIRERTEKIGQEYIGPLVVLTGAEYAA
                     MSFEALHDRICDALRGRRPRLIAETISPDGEAKLVFEDGTVKPLRRPPSS"
BASE COUNT         1479 a         2312 c         2520 g         1588 t
ORIGIN      
        1 ggcggtccac gtaacctgaa cactggtcga ggtgagcgcc gtggccacga cattggtggg
       61 cggggagact gtgacgatcg ttacggtttt ggtgtccgtc actccgacag tgccggcggg
      121 gaacccttct ccgttgttgc ctttggtgtt gtaggcagtg gcccgaagaa ccacgctgcc
      181 cgaagcgccg gcggtatagg tgatggatgc ggtgttggtt gcgctggtaa tcgtgcctcc
      241 ggtgatcgtc cacgcgtatg tgaccgtcgt tccattcgat ggagtgaccg acgccgtgtt
      301 gccggtcgag ctcgcaggga cgctggagga ggccgtgatg gtcgacatcg agaacgtacc
      361 gacagcggtc acgatattgg aggtgccacc gtatggaccg agtggtccgg ccgctcgaag
      421 catgtagtca tccaactcgt gatggctgac ctcggaagcc tgcccaagcg tgtaccggct
      481 ctggggtact tgaccttgga acacgttgtg gcagtagctc atgatcgtgc ccttctccgt
      541 gggcgcggat gagtactgcg cgccggttgc acaggcactg gtgttgccga gtccgtcctg
      601 gtcgatctct ccggtgaaac acatatcgat aaatagctgg ctcgggtggt tcgtccccgc
      661 ggcttcgccg gaggtgatca cgacacagtg ggtatgatgt ccggccaaat tgtgcccgag
      721 ctcgtgcgcg tactcctcga gcggccagta attgttgcct ccggttggca tgccgtacag
      781 cgtcccattg ttcgttgcat tcggatcagg aaccgatccg atgccctgac cgcccaagtc
      841 accaatcgat ccgcaccagg catatgcgcc gccgaagtcg ccgctgttgg agaaatctcc
      901 ttctcctacg acaccctccc atgccacacc actgttggtt gctttgccgc tgagcatgac
      961 aaccgcagag cgtttcaggg agagatgatt cgtgtggtag tagtcaccca actcgtttaa
     1021 cccactgttt gcatcagtcg ccgcccaggg atcgctcccg tttccgccgg tgtaaatgtg
     1081 caggttctgc tggacgacgt tcgtatgaat atcgcggttg tagatcgtcg atacggcgcc
     1141 ggtgaggttc gtgacgtaat tggtcagcgt cgtagaattg ctacccgcat tgatatacag
     1201 ctcggaatcg gtctccacct cgacggtgat cgcatacgcc tgggtgccac tgatgccttg
     1261 cgcgatgacc tgatggttct tcgcatcggt cgcggtcagc aggcgttggt tgacacgggg
     1321 aagagccggc agcttctcga cgccgcacga ccaggtctga cccgtcatcg gcgtctcgtc
     1381 gctcgcgtcc atcgcggtca ggaagtaatc gaagtcgccg tcaatggcgc gaccaggctt
     1441 cggcgttgcc cggcggcgtg acgcgtcgat cgcgaacttg ccgtcgcgtg tggcgatcaa
     1501 tgcgctgatc gcccccgtcg tgccgtcgac cgaaaagtac gcaaacgatt ccggatcgcc
     1561 gttgaccatc cctcggaaga agcgcatcgg aggtggatcg agatacgtga cgctcttgcc
     1621 gtcgtcgacg atgactttcc cacccggcgc ccagacctga aactcttcga gatcgatctt
     1681 caccgtgccg tgcgcgtaga gcggaacgtt gttcaggtgg atctgcttcg ccccgacgct
     1741 gtcccgcaac ggcgagagca catcatcatt gagcttgctg accgtggcgg cggagagagt
     1801 ggtgcagagt cctgcggcca gcagaaacat accaacgctg cgaaccagcg atgaaaacgg
     1861 cagcctcatg ggttctcctg acttgacttg gcccgacgtt ggtacccgta acaaagggaa
     1921 aatgattcta ctcccgccgc aagctcatga gggacgctgc gctcgaaacg cggccaccaa
     1981 caccatgaga atgcaggcac ttaccggact gactcgcgaa gatcctgcat gtgctgcatg
     2041 cggatcgggg cgttcgctgc caagtcggag gagaacggcg cgggagatgc gccaaagacg
     2101 ttgcgagctt cgttgactcc tgcgcgaatc gcggagatgt gtctgcgaac aagcactcgg
     2161 gcggggacgc ccgagtgccc gcgcgcgaga cgcgcgcact ccttgaggag tgcggacgtc
     2221 tcgtccgcgg cattcggggc gtcccgcccc gaatgtttgc gctcgcgaaa gcccgcaaca
     2281 ccccctactc tttcaccgcg accttgtcct tcgcgatgat cttcagcacg gtctttagct
     2341 cgcgcttggc gccgggagat agcgtgtcgt ctgacatgta cggcatcgag cgggcgatcc
     2401 gggtattcgt gagcgtgaat gcgttcggga ccgttgccac gtccacgagg cgggtaccga
     2461 ggagcggggc caggaagggg aggtagttga agcgcccgtg gacgccgtac ttgtagacgt
     2521 cgatggcgcc gaggttgccg aggtcgaagt ccaggatccc ggagcgatgc tcgacgaaat
     2581 cgtgtgcgaa gcggaacatc gcctcattcg agtagtcggg cttgggcctg ggctcgtcgg
     2641 cggcgagcgg catcgcgatg gcgaagagga tgagcaggag cgttgcgcgg gtcatcgtta
     2701 tatctattgt gacgcggctc gccggaaaag gttacgaaaa aaatggttaa cgcttgcgaa
     2761 ttaccgcaga acgctggacg tacggcttct gcgggatcgt ggtgttggtg agcgcgagtg
     2821 cgttcggcat ctccatgctc gcccggaacg cggtgccctg aagcggcgcc aggaacggta
     2881 gatacgccat ggtgtaccgg ccgctgtggg tggtcatgcg gatcgcgccg atctcgacgt
     2941 cgagccgggc ggacgcgtca tcgggggttg tggcctgaag aacgggtcct ggctggggcg
     3001 gtggagtggc cgcggacgga gtgctctgct gcgcgtaaac ggcgctgctg gctaggatga
     3061 ggacggcgat cgatagggtt ctcatgactc aaataacaac gcacgatacg tgccgtgaaa
     3121 gcctgttgag accgccgttt ctggaatgtg tgatgtccgc cacttacgga acggtcaccg
     3181 tcgcccgtgc gaagccgctg ctgttgctgg tttgatcgag gatgccggtg gagaggatgt
     3241 tctcgccgcg cgtcatcagg atcacggtct tgtaggtcat cggtgacatg cggaactcct
     3301 cctcgacctc ggccgggatt ttcactggat gcgggctctt gatcaccttt gacgttctgc
     3361 cgccgctgcc gacgacgatg taaaccgtga agcctcccgc cagatccgcg ttctgcggca
     3421 gcagcgtgat cgtcggcgcg atcgagatct cgacgggaac gcggaactcg ttcccctgct
     3481 gctccggagt gcccgggtga agctcgatct tccaagtgcc gcgaggatcg gagttgttga
     3541 tgttcgcgat cactcgcgcg ctcatctcat cctcggtcga cttcgtggcg tacgtctcgc
     3601 gtgtccggac gttgtactgc gcgttcttcg ttttgacgac gaggttgcgc cgcgcgttgt
     3661 cggcgccgct cgacggcttg aacccgaggg agtagtacga gttgaggtcg tcggcgactg
     3721 tctgaaacgc cacatcgaag ttctggctgt tcgtcaacgc catgccgccg ctgatgcgcg
     3781 ccagtgtctg gtaggccatc gccgtattgg tgaaggtcag gaactgctcc gtttgatcgg
     3841 tggccgagga gcgttcggcg ctgctggtgt cgcgcgtgtc ggcggcgtcg atgatgtaga
     3901 gcgcgacgcc gctgacgctc gccatcttcg ccgcctcttc gatcgagtag tgctgcatcg
     3961 aaccgctctt gccgaggatg ccctcgctcg taaacgtgac attgcccatg taggggagga
     4021 acgcgttgta aagccactgg tacatctcca cgccgggatg ctcggggaga tgcgcgccgg
     4081 cgaagacgag gacgttcttt ccttccatgc cggccagcgc ggtcgtcgtc tggccgaggg
     4141 aagcgagcgt gttctttgac gcgaagatga cttcctccgt atcgctttcg acgaggctga
     4201 gcgacatctg gtagtagtct ttccagggcc gctttccttc tttgccggcg tcgatgtatt
     4261 cgttgacgcg ccgcttgatc tcctcgaccg aatgctgccg gttgaatccg gagctggcgg
     4321 tctgcctgat cgactcgatg ccttgttgca ggagcttcct gtcgctcgtg aatggcgtga
     4381 tgaccttcgt ctgctgcgtg cagaggacga gcatgacctg atcctgcggc cgcacgtcgt
     4441 cgtcgatgaa cttctgcagc gacttgagaa tcttgtcccg ctggaaggga gcgagcgagt
     4501 agttgtcgac gaacatgacg atgttgcggc ggcgggcatc ggcaggagct tcggtcgcgg
     4561 cgggcgctgc ggcgttggat ggctgcggcg tgaccttggc agccgccgcg ggcgccgagg
     4621 gacgaacctc gtaaaggttc gtgatctcct gcttcttgtt gttctcgaag atctcgaaat
     4681 catctttcgt cagcccggtg accggtttgc cggccttgtc gcggacgacg acgtcgatat
     4741 tgacgacgcg cacttcgatc gactcgccgg caggctgctg cgcgggcgag gggagggcgc
     4801 tcaaggcgaa cagcgctgcg attgcgagac gtctcatttg tgtgcggggg tcggtattcg
     4861 ggggtcgggc gtcggagcag agtccgcaac caatcacggt ctcgatctcc gacccccact
     4921 cccgagtcgc gactcccgac gccatggtag cgcagcgggc gtgagcacgt ggccggctca
     4981 acgtaggtcg gactctctag tccgacgtac tcggaaacag tcggactgga gagtccgacc
     5041 tacgttcgtt ccggatgctt cggtgcctcg cgcgcacggc ttccacaggc tgtagacgcg
     5101 atgcgtgaat cgacgtcaac tcccgtaact tcacgtaacg atcttcgcca ctcgtgcggc
     5161 tcaacgtagg tcggactctc tagtccgacg tactcggaaa cagtcggact ggagagtccg
     5221 acctacgttc ctatcgtttt cactcgcaaa accggcgcga acgggtcgac gctactggcc
     5281 tgtttccgag gcacttccca cagccccgta acctccgccg ggctcattcg tctaactgat
     5341 gtatccaaaa aagacggagg cgagtcatgt ccggtgtgca acctatcggg tccttcgaag
     5401 agagtctgac ggatggcagt tccgatcgag tggaccttta ctggcgttgg gaatctgcgg
     5461 tcgtggatcg gggagcaagc gcggggatcg acgtcgatcg agagcaggtc gagatcaaac
     5521 gatgcaggcg tgagatcaag cgctgcaagc gcgcgctcgc gcagctcgac cgcgatacga
     5581 cgcgcttccg gctgcacgtc agcgagagca aacgggagat ccgctaccgc aggcgtgaga
     5641 tcaagcggcg gcggcatgcc cgcagccggc ggagcaagtt cactctccgg ggccggcggc
     5701 agaggaagtt ccctatccaa cggcgcctgc tccgtttcca gcagaaggcc gtcgcgctcg
     5761 cgcggcgtga tggcagccgc ggccagcgtg atgtcagccg gcgtctcgat gatctcgtcc
     5821 ggattctgaa agatctcagc cggcgcgcga aagattctgc tcctcggcga gaaagttctg
     5881 ctccgggatc aaaaagtttt gctcggggag gaaaagattt tgctccggga ggaaaagatt
     5941 ttgctccggg agcaaatgat tctgctccag gaggaaatga atttgctcgg ggagggaaaa
     6001 gttttccacc cggaggaaaa agtttccctc caagaggaaa aagttttgct ccgggagcaa
     6061 atggttttgc tccgggagga caaagttttg ctccagcagg aaaaagtttc cctccaagag
     6121 caaatgcatt tgctccagga gcaaaaagtt ttcctccgcg accacaaagt cctcctccag
     6181 gagcaaaaga ttggggtcct gataagaaac gttttgcacg gaacctgaaa aatctcctca
     6241 ttcgtagaaa gaacgcaatc gccggctccg ggaaaggacc cctgcagtga acgattgcca
     6301 cgtaccgtct ctctggccag gtggtgcccg tgcccctccg ccggtgacac cacgcggatg
     6361 gcgtgtcgcc ttgccgcgtc tgcaccctcg tcgtccggcg ccaatggtgg cgctgacgac
     6421 tccatgaacc ctcttcaaag ggatgcgaca gcagcatcct gaggtacccg atgtctacca
     6481 ccacaccgaa tcccggtacc ccggaggcag gaacgcctgc ccccagtccc caaactctcg
     6541 acgcgcagga gagaatcgct cgcctgcgcg ccatcgctga cgactttccc gacgaagcat
     6601 cgccgaaacc actgacgaag gcggagcgca atcttgcggc tcgtacttcg atcgtcttcg
     6661 tggagaaggc ggcgctgttc gccgaggctg ctccgaacat tggtgttgcg ctttccgtcg
     6721 atacggcggt attgcgtgac gccgttgcct cggaactggc caacggaggg gtaatcgacg
     6781 aaacacggac tctgatctcg cgcatcgagg acgccaacac ccgcaagaag gtcaaagcgg
     6841 tcaaggttgc gcgcggcctc tatcgcgtgg ccaagagcta cgtgaccacc gacctggggc
     6901 acgttgtgac gcctcatgtc gatgaaatga agcgcgcatt gcagcgtccg cgcgcgaaga
     6961 agaagacggc gccagcagag ccgccggcaa cgacgccggc ggcaacgacc agtccgtccg
     7021 attcaacgtc gcaacagaag taaccgaggg gccggaggag gattgtgatt ggctctcacc
     7081 ctccccggct cctcacacga cccgtagtta cgagtcgagc agtgcccgac ccgcggccac
     7141 aaaccgcggg tcggatttta ttacggaatg gtgcgagaga gtcaggcgat ctgttacaga
     7201 gcgcgaacgt cagcagccat cctttcgcga cgaagtcgct gtggagtgcg gtgacgcagc
     7261 caccgctttg aatcacgtgt tcgaaagcgg cagctggcgc tgccgcagtc caaagcgctt
     7321 cgcgcgaaag aatcgcctgc atcgggacgt ttgccgaatc accactgatg agcatttacg
     7381 ccaccctctg gattctcaag ttccccgccc tcggcgattc gtatttcggc tgcgaatggg
     7441 agacggtgat cgcgcaaggc gtgcccggcc acatcggaac gccaacagaa ggccacggct
     7501 acgaagcggg cgatccctac agcagcttcc tcccgcccgc cattgcgatc agtgatgacg
     7561 acgaccaagc gacgccactc cgcgctgtcg tcatcattcg ggagcggacc gagaagatcg
     7621 gacaggagta catcggtccc ctggttgtcc tgacgggagc cgaatacgcc gcaatgtcat
     7681 tcgaagcgct gcatgaccgg atctgcgacg cgcttcgcgg ccgcaggccg cgcctcatcg
     7741 ccgagacgat cagccccgat ggcgaggcga agttggtctt cgaggatggg accgtgaagc
     7801 cgttgcgtcg tccgccgtca agttagttct cgagatgcga cgcagcttgt tggccgcgac
     7861 ttcacgctcg gcgatgaaga cgttcgcgtc gagaaggta
//
LOCUS       DATLVW010000760        12047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_157853, whole genome shotgun sequence.
ACCESSION   DATLVW010000760 DATLVW010000000
VERSION     DATLVW010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12047
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157853"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            640..1971
                     /locus_tag="VNN25_22250"
     CDS             640..1971
                     /locus_tag="VNN25_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HXH94315.1"
                     /translation="MSNTTVDNLQHITVTGVFHITFGTNVRLLWRLRGFKVMGVVLML
                     AFVFAPGYLFAQGCVQPPPGLVGWWPLDETSGTTVLDRSGLGNNGTATGPIGPGSNPK
                     SVAGFVGNGLNFFSGSGMHVPPSPSLNFGPPAVPQNMSFTIDAWIKVHGGPILGNLSL
                     RNQLGYAVAFADNGMLRLEMGTGSSTNTKTWFGPAITPNTWTFIAVVVDRSTQKVTLY
                     TAPVGGPLAATSPPPLIPNSANAYSGSALLIGGCSGDTNGCDTVLDEVEVFNRALTQV
                     EVQSILTAGSAGKCIPAQKKGMTWIHTASNAVAGTITVGCSGCDPYLGDTVCTQQLPL
                     LCIYKPTPPPTSPFQVVPPGVNNSNQYNLWSGGVVATTPPHVGTFAHSTDATAYCQAQ
                     FGPSWRVAEFHEGWAWNFQAYGGTVSAPAVPSTRFWVHINDQPAANCWQTP"
     gene            2591..3031
                     /locus_tag="VNN25_22255"
     CDS             2591..3031
                     /locus_tag="VNN25_22255"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH94316.1"
                     /translation="MTREGNQKYYQAQRESPVFPELHGLIVKTVGVVDPLRAALAPLT
                     SRIHLAFVFGSVAKARERAGSDIDLLVVTDNLAYADIYAALETAEKSLARTINPTVFT
                     SAEWKSKRARNDSFAARITAQRRLFVIGNDDGPGMNPPIALRYS"
     gene            3040..3672
                     /locus_tag="VNN25_22260"
     CDS             3040..3672
                     /locus_tag="VNN25_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr and Tyr protein phosphatase"
                     /protein_id="HXH94317.1"
                     /translation="MSEDALPTSTLPRWERLRMFSFWSFWIGVAFFGVYPTMNWITSL
                     RAARLHLYVPAELAIPFVPQFIWAYLSMYVLFFLPLVFVPTARMPPLGKQLIAGTLIS
                     AAFFLLLPAELGFVRTVPEAAPYASLYAGIFGVDRPFNLVPSLHVIYSSAITLACADF
                     ARPRARFGLLLWLVVIVASTLLVHQHHLLDVAAALAIVWYLHRRFEVIHD"
     gene            3665..4255
                     /locus_tag="VNN25_22265"
     CDS             3665..4255
                     /locus_tag="VNN25_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH94318.1"
                     /translation="MTKSLLIACLFLTLPLYAAEEADHEIHQELRGILTTVQTAINTG
                     NYDAMLPVLSKDIRATTITQEVMSGHEQVSAYFKHWFGPGGFLKKLDMTFTADALTEL
                     SPDKTWGVVRGSGMERYTLADGRQYDMPTRWTATVVKEADGKWRLRTIHFGTNFLDNP
                     ILTEAKHAVVKYAAVAGGGGLALGLLLGFLLGRRKQ"
     gene            complement(4223..5182)
                     /locus_tag="VNN25_22270"
     CDS             complement(4223..5182)
                     /locus_tag="VNN25_22270"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXH94319.1"
                     /translation="MLKSGAVRSSAPESLNWILLGAAAAATSGCLWLASHGRWWSMLA
                     AAIVFAFVNNVPFALMHEAVHGVAANTPRRNYWFGFLASCMFPTSFSMQQVAHLGHHA
                     RNRTDSDLYDYYLPGQSRLVRNLWLYGGNLFGLYWFCIPLSNLIYLMGTPVYRSRFFV
                     ERVAPRIGFGPHVRDIAQLPPLRIWLEMLCAFACQAAIYVGLGLDWKGWLLAHWFFAL
                     HWSALQYVDHAWSARDVGHGAWDLRVFAPVRWLTLNYQYHRAHHSNPGAPWTELPALA
                     VHDSREPTFLRIYWSLWRHGVQPAPPMGAPAQRSLFAAAEQEA"
     gene            complement(5176..5841)
                     /locus_tag="VNN25_22275"
     CDS             complement(5176..5841)
                     /locus_tag="VNN25_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94320.1"
                     /translation="MKRALSVYQYAAPLVLAPLSFLLWKREYAGNLKLTATAWLIPIL
                     WAYIVPGIGTNVLNVWEFDTRLRLGRFRPHHGFVFGSATAMIAWLVHSSPARDLWDVA
                     RNALILCSVIGFWNLLYEVKALQSGILRVYNQPWADGRDETAIALDYAPWFFGGFGAL
                     YGAGIGLMEWLDGRGALSMPVFAAAFGLILVLTLTVPAGGYILQSIRRHGHWGTRPVK
                     RSC"
     gene            complement(5838..7169)
                     /locus_tag="VNN25_22280"
     CDS             complement(5838..7169)
                     /locus_tag="VNN25_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH94321.1"
                     /translation="MKITFIKPNIGRMEHSLYVDEGRMEPLMLAVLAGMTPPDVECVL
                     YDDRMESIPFDEPTDLAAITVETYTARRAYEIAAEYRKRGVPTILGGMHITLLPEEAA
                     LYADSIFLGDAETIWDQVVEDARQHKLKPRYQGPPGVGQIGRVLPRRDLFKGKGYLPL
                     TLMQFVRGCRFACSFCAVSQYFDKKHYVRAIDDVLREIEAQDRKFIFFIDDNIASDQK
                     ALAEFCHAVTPLKLHWVSQASLDVTRNPTLMKLLERAGNWGNVTGFETITPESLRESR
                     KSPNIPRFTKYKEEVRILREHGMQSWAAFTLGYDHDTKESIEATVDFALESRFTLAAY
                     NILMPYPNTPLYHKLGKEGRHLYGGKWWLDPTYRFNRAAFKPSLMSPDELTDACHAAR
                     TRFNSIPSLFHRFADVQTHLRSLSRMGAFWKYTLLFKKEIPKKHGMRFGLQ"
     gene            complement(7179..8480)
                     /locus_tag="VNN25_22285"
     CDS             complement(7179..8480)
                     /locus_tag="VNN25_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH94322.1"
                     /translation="MKITLIRPNLGDYRSSDAMPPLAMGILAARVTGHDIVFYDEKAE
                     TVPVGDSPDLVAMSVETYTARRAYAIADGYRSRGVPVVMGGYHPTFLPDEALEHADAV
                     VIGDAEGAWERLLADLEAGRLQQKYAGSNDAPLTDYRIDRSIYKGKNYAPVELVQWGR
                     GCRFSCDFCSIHSFYGTSLRTRPLEGLIAEIEALPAGRLLFFVDDNLFGRKSDLLALL
                     DAMTPLKRRWSCQITIDVARDEALLDRMAAAGCGFVLIGFESLNPESLKQMHKAWNNV
                     SGSYSHVVRALHARNIGIYGTFVFGYDADTKESIERTVDFALEARLEIANFNLLTPTP
                     ASALYDRLASEQRLLLPKWWLDPNYRYGDAIFEPRGMHARELTEGVFEARRRFYSWRA
                     IGRRVLGAETSFSPFRAGLTTVANVISRREIYKKQYRLLGM"
     gene            complement(8477..9511)
                     /locus_tag="VNN25_22290"
     CDS             complement(8477..9511)
                     /locus_tag="VNN25_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94323.1"
                     /translation="MTTFRLATENDDALIRSLLRGNPMPTWVDMAIEREPSFFAGKDL
                     VGRDWAVIAEEEGDVVGMYTASVLPAHVDRQPERLGYLGGLRVNAGYRRRIRYLRDGY
                     ASIRRLAPVTGTIPWWFTVVASENTTARRLLESGVAGLPRYHFQGDYVTFAVATSRGK
                     RRNLWRRATPDDVAHIVAFYNEYAARFQCSPVLTEDVVRRIGVEQFFLYEDRGIAALW
                     DQRAFKQIVARRYHSPLGTLRPIYNLYASLLRQIPLPREGGALDQTSIAFLALNDEEQ
                     PNAGILLQDLLSHCKTRAASIGLHAKHPLVGVIRKMKPLQYPARVYAVCFEGDAPPNG
                     RPVQPEAALL"
     gene            complement(9508..10854)
                     /locus_tag="VNN25_22295"
     CDS             complement(9508..10854)
                     /locus_tag="VNN25_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006684991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH94324.1"
                     /translation="MKLTFVHPAVGHRAGESYVRTWQMESLPIAVLAGLTPSDIETTF
                     YDDRMETIDYDAPTDAVAIPIETYTAARAYQIASEFRSRGVPVVMGGFHATLVPDEVA
                     RFADAVVTGEAEGAWPRVVDDLRHGTLQHRYHGAQQPLANIKIDRRLFRGKRYLPIGL
                     VETGRGCHFPCEFCAIQTFFSRTYRCRPTDDVIAELSSLKADKRVFFFVDDNFAGDLK
                     SGKPLLAELAKLDIRWITQMSIDAAHDEDFLAELHRAGCRGVLIGFESLDEDNLKLMN
                     KRFNTMKSGFRGALENLRRHRIFVYGTFIFGYEHDTHASFDEAVDFATEEGMYIAAFN
                     HLTPFPGTPLYKRLQNEGRLRFEAWWLDPEYRYNAVPFEPKSLTPDEVTAGCVAARRR
                     FYGWPNILKRSTRHMRDGFVFRNFFLVNALHRNEISQRNGYPLGDENWRGKLLEVQ"
     gene            complement(10851..>12047)
                     /locus_tag="VNN25_22300"
     CDS             complement(10851..>12047)
                     /locus_tag="VNN25_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978563.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXH94325.1"
                     /translation="LDADLIGMTVITGTARRSYELARTFRAQGKTVVLGGPHVTLLPE
                     EAAIHADAIVVGYAEDTWPELLRDFARGALKHEYRMAPGFSLDRPDMPFARRELFNRR
                     HFLTQAVFEATRACGHDCEFCIVPTAWGSRQFQKPVSWIVEDIRRVGQKQILFVDLNL
                     VADKVYARELFTALIPLKVRWFGLSTVLIAHDRELMELMARSGCRGLLLGLESIISDS
                     LGDANKRFNGSVDYKALIADLHDLHIAIQGCFVFGLDHDPLEVFDITADFAIDNGIDL
                     PRFAILTPFPGTPLHQRLAAENRILTRDWELYDGQHVVFQPRNMTPQQLSAGHERAWK
                     KVYRYNAIARRLWKARAFEPLGLAANLGYRFYAHHLQRFYNCDWHLSAMPALTKSLSK
                     SLSKAV"
BASE COUNT         2399 a         3619 c         3683 g         2346 t
ORIGIN      
        1 cagcagcggt tcgcgctcac ccggtcactc ctggtcgtcg aaccgccact gatcgaagct
       61 gccgtcgccc cctgttcggg ggttaccact ggaacggtat cgacctgatc tcgccatttg
      121 tgccagcgcc atcaatccct ccgcttagtg gctaataacg cggcccattg attttcacaa
      181 agatccgcgg cactcgtgcc agcgattgtc agagccgcgc agaatcacgc tcaagcgtga
      241 tgaacatcac gcctccaccc agcgaatccg catggcacca ccgtacacct cggcgcgctg
      301 aggaacgagg tgccgatttc aatcgttatt gagcctcgcc tcaactgcct aaatcgtgaa
      361 ctgaccaatc ttcgccggtt gcacatgcga ccgcaaggac gcatgttgcg tcaggaactc
      421 cttactactg gtagtgtgca tcacgacaca atcgtgctcg aattgtctgt tggagagctt
      481 caaatgagcg actgcgatgg agtattacgc gaatcttcag ctgcgccaca acggcggaga
      541 tcctgcgccg ggcgcccgcc ttggaaggaa acaaatacgc cgcgatcgtc aggatatcga
      601 cgcggaatag caaccaaaac agagcaagga gcaccccaaa tgtcgaatac gacggtagat
      661 aacctacaac acatcacagt gactggagtg tttcatataa cgttcggcac gaacgtccgc
      721 ctgctgtgga gattgcgcgg tttcaaagtc atgggagtgg tcctgatgct cgcattcgtg
      781 ttcgcgccag ggtatttatt cgcacaaggc tgtgttcaac caccgcccgg cctggtgggt
      841 tggtggccgc tggatgaaac gtccggtacc acggtcttgg atcggagcgg tctcggcaat
      901 aacggcactg caactggccc gattgggccc ggcagcaatc ccaagtccgt tgcgggattt
      961 gtcggcaatg gattgaactt cttttccgga agtgggatgc acgttccacc cagccctagc
     1021 ctcaatttcg ggccgcccgc agttccccaa aatatgagct tcaccatcga cgcttggatc
     1081 aaggttcatg gcggtccgat tttaggtaat ctcagtctga gaaatcagtt aggctatgct
     1141 gtcgcctttg ccgacaacgg catgttaagg ttggagatgg gcactggctc atctacgaac
     1201 acaaaaacgt ggttcggacc ggcgattact cccaatacat ggacatttat tgctgttgtc
     1261 gttgatcgaa gcacgcaaaa ggtaacttta tatacagcac ctgttggtgg ccccttggcc
     1321 gccacttctc cccctccctt aatccctaac agtgcgaacg cttacagtgg ttccgcattg
     1381 ctgatcggtg gatgctcggg tgataccaat ggctgtgaca ccgttctgga cgaggtggag
     1441 gttttcaatc gtgccctgac gcaagtagaa gtccagagca ttctcaccgc gggcagcgcc
     1501 ggcaagtgca tcccggcaca aaagaaaggc atgacgtgga ttcatactgc atccaacgcc
     1561 gttgctggca cgatcacggt cggttgcagt ggttgtgacc cctaccttgg ggacaccgta
     1621 tgcacccagc agttgccgct tctgtgcatc tacaagccga cccctcctcc cacgtcccca
     1681 tttcaggtgg ttccaccggg cgtgaacaat tccaatcagt ataatctgtg gtcgggtggt
     1741 gttgttgcga cgactcctcc ccatgttggc acatttgcac acagcacgga tgccaccgcc
     1801 tactgccagg cgcaattcgg cccctcctgg cgtgtggccg aatttcacga aggctgggcc
     1861 tggaacttcc aggcttacgg cggcactgtt agcgcccctg cggtgccatc cacgcgcttc
     1921 tgggttcaca tcaacgatca accagcagct aattgctggc aaacaccatg acgatcagac
     1981 gaaatgtagg cgcgtggcag gttcgtatgg ccatgccaac cctagcgcac cataccgttg
     2041 ccgatcggga cggcgggccg tgtgtcttcg cttgttcgag ggtcgagcgc atctgcttta
     2101 tcgaccgtcg gcccgcctcg acgcgtcggc accttccaag tcatcagata ggcgagatag
     2161 gtgtcagtct ggtggcggtt tcgccgccag ccgacgcatc acggtgcctc cgaacgcttt
     2221 gcgacagact tcaacaagta ccgcatggct gcagacattc cggtagctcc ccaacagcct
     2281 tctaagctgt gggtcgcagg ttcgatccct gccggggaca ccagcggcgc attcgttcca
     2341 caatcacccg tcttgcaatc gttcccaaaa cgggtacgat cgttcccgaa atgagaacgg
     2401 tagtccgcaa gccgccccga agtgctgtag cgacggcgct tttcactccc gtccagcaac
     2461 gagtgctggg gctctcttcg gacagccgga gaggcggttc cagagcgcgg agctgatccg
     2521 gcttgcggga agtggcaccg gtgcggttca ccgccaactt cagcgactgg cggcaaccgg
     2581 tctcgttcga gtgacccgcg aaggcaatca gaagtactac caggctcaga gggagtcacc
     2641 tgttttcccg gaacttcatg gcctcatcgt caaaacggtc ggcgtcgtcg atcctctgcg
     2701 cgcggccctg gcaccgctca cgagccgcat ccatctcgcc ttcgttttcg gatcggttgc
     2761 caaagcgcgc gaacgcgccg gcagcgacat cgacctcctc gtcgtcaccg acaatctcgc
     2821 ctacgcggac atctatgcag cgctcgaaac ggccgaaaaa agcctcgccc gaacgatcaa
     2881 tcccaccgtc tttacgtccg ccgaatggaa gagcaagcga gcccggaacg attcgttcgc
     2941 agcgcgaatc accgcgcaac gacggctctt tgtaatcgga aacgatgatg gcccaggaat
     3001 gaatccgcca atcgccctcc gctatagttg aagtccctca tgtccgagga tgcgcttccg
     3061 acgtcgaccc tgccgcgctg ggagcgcttg cggatgttct ccttctggtc gttctggatc
     3121 ggcgtcgcgt ttttcggcgt ctatccgacg atgaactgga tcacgtcgtt gcgcgcggcg
     3181 cgcctgcatc tctacgtgcc ggcggaactg gcgattccgt tcgtgccgca gttcatctgg
     3241 gcttacctgt cgatgtacgt gctgttcttc ctccctctcg tcttcgtacc caccgcacgg
     3301 atgcctcccc tcggcaagca gctcatcgcc ggtaccctga tcagcgcagc gttctttctg
     3361 ctgctgccgg cggagcttgg atttgtgcgc acggtgccgg aagccgctcc gtacgcctct
     3421 ctctacgccg gcatctttgg ggttgaccgc cccttcaatc tcgttccgtc gctgcacgtc
     3481 atctactcca gcgccatcac actggcatgt gcagacttcg ctcgccctcg ggcccgtttt
     3541 ggattacttt tgtggctcgt cgtgatcgtc gcctcgactc tactcgtcca tcagcaccat
     3601 ctgctggacg tcgcggccgc gctggcgatc gtctggtact tgcatcgtcg ttttgaggtg
     3661 atccatgact aagtccctgc tcatcgcttg cttgttcctg acgcttccgc tctatgccgc
     3721 ggaggaagcg gatcatgaga ttcatcagga gctgcgcggc atcctcacca ccgtgcagac
     3781 cgcgatcaac accggcaatt acgacgcgat gttgccggtg ctgagcaagg acatccgcgc
     3841 cacgacgatc acgcaggagg tgatgtcggg ccacgaacag gtgtccgcat acttcaagca
     3901 ttggttcgga ccgggcggct ttctcaagaa gctggacatg acattcaccg ccgacgcgct
     3961 caccgaactg tcgccagaca agacgtgggg tgtcgtgcgc ggctccggaa tggaacgcta
     4021 cacactcgcc gacggccgcc agtacgacat gccgacgcgg tggactgcaa ccgtcgtgaa
     4081 agaagccgac ggcaaatggc gcctccgcac gatccatttc ggaacgaact ttctcgacaa
     4141 cccgattctc accgaggcaa aacacgctgt ggtcaagtac gcggcggtcg ccggaggcgg
     4201 gggactcgcc ctcggcctcc tcttaggctt cctgctcggc cgccgcaaac agtgaccgct
     4261 gcgccggcgc gcccatcgga ggcgccggtt gaacgccgtg acgccacaaa ctccaataga
     4321 tccggaggaa ggtcggctca cggctgtcat gcacggcgag agcgggcagt tcggtccagg
     4381 gcgcgccggg gttcgaatga tgcgcgcgat ggtactgata gttgagcgtc agccatcgta
     4441 ccggcgcgaa cactcttaaa tcccatgcgc cgtgtccgac atcgcgcgca ctccaggcgt
     4501 gatcgacata ttgcagcgca gaccagtgca gagcgaagaa ccagtgcgcc agcagccaac
     4561 ccttccaatc gagcccgaga ccaacataaa tcgcggcttg acaggcaaac gcacagagca
     4621 tctccagcca gatacgaagg ggcggcaact gtgcaatatc ccgcacatgc ggaccaaacc
     4681 caatcctggg tgcaacgcgc tcgacgaaga agcgcgagcg ataaaccggc gtccccatga
     4741 gataaatcag attggacagc gggatgcaga accaatagag accaaagaga ttgccgccat
     4801 agagccagag attgcggaca agacgcgact ggccgggaag atagtagtcg tagagatccg
     4861 agtcggtgcg gttgcgggcg tggtgcccga ggtgagcgac ttgctgcatg gaaaaggagg
     4921 tcgggaacat gcaactggcg agaaacccga accaatagtt gcggcgcggt gtattcgcgg
     4981 ccacaccgtg aaccgcctcg tgcatcagcg cgaacggaac attgttgacg aaggcaaaga
     5041 cgatggcagc cgcaagcatt gaccaccatc ggccgtgact agccagccag agacagccgg
     5101 atgttgccgc tgcggccgcg ccgaggagta tccagttcaa gctctccgga gcggaactgc
     5161 ggactgcgcc cgatttcagc atgatcgctt caccggacgc gtgccccaat gcccatggcg
     5221 gcggatcgat tgcagaatgt acccgcccgc cggcacagtg agtgtcagaa cgagaatcag
     5281 gccgaatgcc gccgcgaata ccggcatcga cagcgctcct cgtccgtcga gccattccat
     5341 cagtccgatt ccagcaccgt aaagagcgcc gaatccgcca aagaaccagg gcgcgtaatc
     5401 gagtgcgatg gcggtctcat cgcggccatc ggcccacggc tggttgtaga cacggaggat
     5461 tccactctga agtgctttga cttcgtaaag gaggttccag aagcctatta cggagcagag
     5521 gatcagcgca tttctggcca catcccacaa atcgcgtgcc ggtgagctgt gaaccagcca
     5581 ggcaatcatc gccgtcgccg agccgaagac gaagccgtgg tgcggccgga agcgccccag
     5641 ccgaagccgc gtgtcgaact cccagacgtt caacacgttc gtaccgattc ccgggacgat
     5701 atacgcccat agaatgggga tgagccatgc cgtggcggtg agcttcaggt ttcccgcgta
     5761 ctcgcgcttc cagaggagga aggacagcgg cgctaggacg aggggcgcgg catattgata
     5821 aaccgacaac gcgcgtttca ttgcagtccg aatcgcattc cgtgcttctt ggggatttcc
     5881 tttttgaaca gcagcgtgta tttccagaac gctcccatcc gcgacaggga acgcaagtgc
     5941 gtctgcacat cggcaaagcg atgaaagagt gacgggatgg agttgaagcg tgtgcgggcg
     6001 gcgtgacagg catccgtgag ctcgtccggg ctcatgagcg aaggcttgaa cgccgcacga
     6061 ttgaagcgat acgtcggatc gagccaccac ttcccgccgt acaggtgccg tccctctttc
     6121 cccagcttgt ggtacagcgg cgtattggga tacggcatga gtatgttgta agcggccagc
     6181 gtgaaccggc tctccagtgc gaaatcgacc gtcgcctcga tcgactcctt cgtgtcgtgg
     6241 tcgtatccga gcgtgaaggc cgcccagctc tgcattccat gctcgcgcag gatgcgcact
     6301 tcttctttgt attttgtgaa gcgcgggatg ttcggcgact tgcgcgattc gcgcaggctc
     6361 tcgggcgtaa tggtctcgaa tccggtgacg ttaccccagt tgccggctcg ctccagcagt
     6421 ttcatgagtg tcggattgcg ggtgacgtcg agactggcct gactgaccca gtgcaacttc
     6481 agcggcgtga cggcgtggca gaactcggcg agcgccttct ggtcgctggc gatgttgtca
     6541 tcgatgaaaa agatgaactt cctgtcctgc gcctcgatct cgcgaaggac atcgtcgatg
     6601 gcgcgaacgt agtgcttctt atcgaaatac tggctcaccg cgcagaagct gcaggcaaac
     6661 cggcaaccac gtacgaactg catcaacgtg agcggcagat agcctttgcc tttgaagaga
     6721 tcgcgccgtg gaagaacgcg gccgatctgg ccgacgcccg gagggccctg gtatcgaggc
     6781 ttgagcttgt gttgccgcgc gtcttcgaca acctggtccc agatcgtctc cgcgtcgccg
     6841 agaaagatgc tgtcggcgta caaagcggct tcctcgggaa gcagcgtgat gtgcattcca
     6901 ccgagaatcg tcgggacgcc gcgtttgcgg tattcggcgg cgatctcata agcgcgccgc
     6961 gcggtgtatg tctcgacggt gatggccgcg aggtcggtcg gttcatcgaa cggaatgctc
     7021 tccatccggt cgtcgtagag gacgcattcg acgtccggcg gcgtcatccc ggcgagcacg
     7081 gccagcatga gcggctccat gcgtccctcg tcgacgtaga ggctgtgctc catgcggccg
     7141 atattaggtt tgatgaacgt gattttcatg ttgggtgctt acatgccgag gaggcggtat
     7201 tgcttcttgt agatctcgcg gcgggagatg acgttggcga cggtggtaag gccggcgcga
     7261 aatggcgaga aggacgtttc ggcgccgagc actcggcgcc cgatggcgcg ccaactgtaa
     7321 aagcggcgcc gcgcttcgaa gacgccttcc gtgagctcgc gcgcgtgcat gccgcgcggc
     7381 tcgaagatcg cgtcgccgta gcgataattg ggatcgagcc accacttcgg caatagaagc
     7441 cgctgctccg atgccagacg gtcgtagagc gcggaggcgg gcgtgggagt gagcaggttg
     7501 aagttcgcaa tctccagccg cgcctcgagt gcgaaatcga ccgtgcgctc gatgctctct
     7561 ttcgtatcgg cgtcgtagcc gaaaacgaac gtgccgtaga tgccaatgtt tcgcgcgtgc
     7621 agcgcgcgga cgacgtggct gtacgagccg ctgacgttgt tccatgcttt gtgcatctgc
     7681 ttgaggctct cgggattgag cgattcgaag ccgatgagca cgaacccgca accagcggcg
     7741 gccatccgat cgaggagtgc ctcgtcacgc gctacgtcga tggtgatctg gcacgaccag
     7801 cggcgcttca acggcgtcat cgcgtcgagc aacgccagaa ggtcggactt cctgccgaac
     7861 aaattgtcgt cgacgaagaa caacaggcgc cccgctggca aagcctcgat ctccgcgatg
     7921 agaccttcga gcggccgcgt acgcagcgag gtcccgtaaa aactgtggat ggagcagaag
     7981 tcgcacgaaa agcggcaacc gcgtccccac tgcacgagct cgaccggcgc gtagttcttg
     8041 cctttgtaaa tggagcggtc gatgcggtaa tcggtaagcg gcgcgtcgtt gctaccggcg
     8101 tatttctgtt gaagccggcc cgcttcgagg tcggcgagca gccgctccca cgccccttcg
     8161 gcgtccccga tcacgaccgc gtcggcgtgc tccaacgcct cgtccggcag gaacgtcggg
     8221 tggtacccgc ccatgacgac cggcacgccg cgcgaacgat aaccatcagc gatggcgtaa
     8281 gctcggcggg cggtataggt ctcgacgctc attgccacga gatcgggcga atcgcctacc
     8341 ggaaccgtct ctgccttttc gtcatagaag acaatgtcgt ggccagtcac gcgcgccgcg
     8401 aggatgccca tcgccaatgg cggcatggcg tctgacgagc gatagtcgcc gaggttgggg
     8461 cggatgaggg tgatcttcat agcagcgccg cctccggctg gaccggccgt ccgtttggcg
     8521 gcgcgtcccc ttcgaagcag acggcataga cccgcgccgg atattgcagc ggcttcatct
     8581 ttctgatgac gcctacaagc gggtgttttg cgtggagacc gatgctggcc gcgcgggttt
     8641 tgcagtgcga gaggagatcc tgcaagagaa tgccggcatt cggctgctct tcgtcgttga
     8701 gcgcgagaaa ggcgatcgat gtttggtcga gagcgccgcc ctcgcgcggc agcggaatct
     8761 gacgcaggag cgaggcgtac aggttataga tcggacggag agtaccgagc ggtgaatggt
     8821 atcgccgggc gacgatctgc ttgaaggcac gctgatccca cagtgccgcg atgccgcggt
     8881 cctcgtaaag aaagaactgt tcgacgccga tgcgacgcac aacatcctct gtcagcactg
     8941 gcgagcattg aaaccgcgcg gcatactcgt tgtagaaggc cacaatgtgt gcaacgtcat
     9001 ccggcgtcgc acggcgccac aggttccgcc gctttccacg ggaggtcgcg accgcaaacg
     9061 tgacgtagtc gccttgaaag tgatagcggg gaagaccagc cactcccgac tcgagaagcc
     9121 ttcgtgctgt cgtgttttca gatgccacga ccgtgaacca ccaggggatt gtgcctgtga
     9181 ccggggcgag ccttcgaatt gacgcatagc cgtcgcgcag atagcggatg cggcggcggt
     9241 aacctgcatt gacgcgcagg ccgccgaggt agccgagccg ctccggctgt cgatcgacgt
     9301 gggccggtag aacggacgcc gtgtacatcc cgacgacgtc gccttcctct tcggcgatga
     9361 ccgcccaatc gcgtccgacc aggtccttgc ccgcgaagaa cgatggctcg cgctcgatgg
     9421 ccatatcgac ccatgtcggc atcggattac cgcgcaagag cgagcggatg agagcatcgt
     9481 cgttttccgt cgcaagcctg aaggtcgtca ttgaacctcc agcagtttgc cgcgccagtt
     9541 ttcgtcgccc agtggataac cattgcgttg cgagatctcg ttgcggtgga gcgcgttgac
     9601 gagaaagaaa ttgcgaaaga cgaagccgtc gcgcatgtgg cgcgtgctgc gcttcagaat
     9661 gttgggccat ccgtagaagc ggcggcgcgc ggctacacag ccggcggtca cctcgtccgg
     9721 cgtgagtgat ttcggctcga acgggaccgc gttgtagcga tactcagggt cgagccacca
     9781 cgcctcgaag cgtagccgcc cctcgttctg caaccgttta tagagcggcg tccccggaaa
     9841 cggcgtcaga tggttgaacg ccgcgatgta catcccctcc tccgttgcga agtccaccgc
     9901 ttcgtcgaac gaggcgtgcg tgtcgtgttc atagccgaag atgaaggtgc catagacgaa
     9961 gatccggtgc cggcgcaggt tttccagtgc cccgcggaaa ccgctcttca tcgtattgaa
    10021 gcgcttgttc atcagtttca gattgtcctc atcgaggctc tcgaatccta tgagcacgcc
    10081 gcggcagccg gcccgatgca attcggcgag aaagtcttcg tcgtgcgcgg cgtcgatgct
    10141 catctgcgta atccagcgga tatcgagttt cgccagctcg gcaagcagcg gcttgcctga
    10201 cttcagatcg cctgcaaagt tgtcatcgac aaagaagaag acccgcttgt ctgctttgag
    10261 gctcgacaac tcggcaatga catcgtccgt tggccggcag cgatatgtgc gcgagaagaa
    10321 tgtctggatc gcacagaact cacagggaaa atggcagccg cgtcctgttt cgacgagccc
    10381 gatcgggaga tagcgcttac ctcgaaagag ccggcggtcg attttgatgt tggcgagagg
    10441 ctgctgtgcg ccgtgataac gatgttgcag cgttccatgc ctcaaatcat cgaccacacg
    10501 cggccacgcc ccctcggcct cccccgtaac tacggcgtcg gcgaagcgcg ccacttcatc
    10561 gggaaccagg gtcgcgtgga aaccgcccat gaccaccggc actcctcggc tgcgaaactc
    10621 gcttgcgatc tggtaggcac gcgcggcggt ataggtctca atcgggatcg cgacggcatc
    10681 cgtgggcgcg tcgtagtcga tcgtctccat ccgatcgtca tagaacgttg tttcgatgtc
    10741 cgagggcgtg aggccggcaa ggaccgcgat cggcagtgac tccatctgcc acgtgcggac
    10801 gtagctctcg ccggcgcgat ggccgaccgc cggatgaacg aacgtcagct tcatacggcc
    10861 ttgctcaatg acttgctcaa tgactttgtc agggcaggca tcgccgagag gtgccagtcg
    10921 cagttgtaga agcgttgcag gtgatgggcg tagaagcggt agccgaggtt ggcggcgagg
    10981 ccgagcggct cgaaggcgcg cgctttccag aggcggcggg cgatggcgtt gtagcgatag
    11041 accttcttcc acgcgcgctc gtgtccggcg gagagttgct gcggagtcat gttgcgcggc
    11101 tggaagacaa cgtgctggcc gtcgtaaagc tcccaatcgc gtgtgaggat gcgattctcg
    11161 gccgcgaggc gctggtgcaa tggcgttccc ggaaacggcg tcaggatcgc gaatcgcggc
    11221 aggtcgattc cattgtcgat cgcgaagtcg gcggtgatgt cgaagacctc gagcggatcg
    11281 tgatcgagtc cgaagacgaa gcagccctgg atggcgatgt gcaggtcgtg aaggtctgcg
    11341 atgagcgcct tgtaatcgac ggagccgttg aagcgcttgt tggcgtcgcc gaggctgtcg
    11401 gaaatgattg actcaagccc gaggaggaga ccgcggcagc cggagcgggc catcagctcc
    11461 atcagctcgc ggtcgtgcgc gatgaggacg gtcgacagcc cgaaccagcg aactttcagc
    11521 gggatcaaag cggtgaagag ctcacgggcg tagaccttgt cggcgacgag attgagatcg
    11581 acgaagagga tctgcttctg tccgacgcgg cggatgtcct cgacgatcca cgagaccggc
    11641 ttctgaaact gccggctgcc ccacgcggtg ggcacgatgc agaactcgca atcgtggcca
    11701 caggcgcggg tcgcttcgaa tacagcctgc gtgaggaaat ggcgccgatt gaaaagctcg
    11761 cgccgcgcaa agggcatgtc gggacgatcg agggagaagc cgggcgccat ccgatattcg
    11821 tgcttcagag cgcctcgtgc gaaatcgcga agaagctcgg gccaggtgtc ttccgcgtaa
    11881 ccgaccacga tcgcatcggc gtgaatcgcc gcttcctcgg gcagcagcgt aacgtgcgga
    11941 ccgccgagaa cgaccgtctt cccctgcgcg cggaacgtgc gcgcgagctc gtacgagcgt
    12001 ctggcagtgc cggtgatgac cgtcatgccg atgaggtccg cgtcgag
//
LOCUS       DATLVW010000761         3972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_280987, whole genome shotgun sequence.
ACCESSION   DATLVW010000761 DATLVW010000000
VERSION     DATLVW010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280987"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VNN25_22305"
     CDS             <1..345
                     /locus_tag="VNN25_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXH94326.1"
                     /translation="YPYVSILFLGCVAAAFCSLSLKNVLQAILSIRAVIPFMAQIVGA
                     VLLRQREPDRPRPFRMWLYPLPAIIALGLWGYIVTSPQKGLKAGGLYVMAAGSLFYLG
                     RWWLEKRKRESL"
     gene            complement(436..825)
                     /locus_tag="VNN25_22310"
     CDS             complement(436..825)
                     /locus_tag="VNN25_22310"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HXH94327.1"
                     /translation="MRRTASALLVLLMLSCSGITIYEQQEMDTLYFGTQKPDGSAPVT
                     ETEWQRFLEDEITTRFPDGLTTWDASGRWRDAHGLVEHERTHIVQIVHRANGEEEGRI
                     AAIIALYKKNFAQESVFRVRSDVWMPR"
     gene            845..2329
                     /locus_tag="VNN25_22315"
     CDS             845..2329
                     /locus_tag="VNN25_22315"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH94328.1"
                     /translation="MRRYVIAVSIFLITVTASAQLSDAVIEKDVRDTMNAWHLPGLAI
                     AVVQNDRVIFIKGYGIKEIGKDDPVTADTIFEIGSTTKAFTATAMAMLADEKKLSWDD
                     PVRNYVAGFHISDPCTDSLITLRDIASHRSGVARHDELWDDSDFSRDELIKRIGAMKI
                     SKPIRTAYQYNNLMFVTAGEAAASAAKMPWEDLIRTRIFAPLGMTESRIAFADWPASD
                     HATGHHWDDKGERAAIQTMRNYDALAPAGTIKSSAHDMAQWLRFQLAGGVIDGKRLLS
                     EIALDETHTPQTIIPLGTSTRALYPDTNFETYALGWNASDYHGELLVAHAGALNGFRT
                     NVALLPKRHLGIVILENVGRGSALAALRNTIIDQFLGAPTRNWNSDLLEVEKSATFEA
                     NLKRGEREAKRATAPKPSHDLTAYAGTFHNAGFGDATIAIVNGALVLHWERLTIPLVH
                     ENYDTFSAIDTVEDIDESVPFRLGSDGDVKAFTLFGEEFVRGQV"
     gene            2413..3705
                     /locus_tag="VNN25_22320"
     CDS             2413..3705
                     /locus_tag="VNN25_22320"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXH94329.1"
                     /translation="MNDKRIVIIGGGFGGLYAAKALRRAPVQITLVDRRNYHLFQPLL
                     YQVATAALNPSDIAAPIRSVLRKQENVGVILGDATSIDLDRKLIQLADGELAYDILII
                     ATGATHSYFGHAEWEENAPGLKTIEDALEIRRRVLLAFEAAEREDDPQRQRAWLNFVV
                     IGGGPTGVELAGALSEIARQTMLRDFRRINPSSARVILIEGMDRVLPPYPPDLSAKAA
                     AQLQSLGVEVMTKTTVTRVSDHEVAAGEMIIPTRTVLWAAGVQASPLARSLGAPLDRA
                     GRVLVAPDLSVPEHPEVFVIGDLAAVTQSNGKPVPGVAPAAIQEGEHTAKNIVRMLNA
                     QPTLPFHYFDKGSLATIGRAAGVADFGRIHLSGFIAWAAWLGVHIFFLIGFRNRLLVI
                     LQWAWAYVTFQRGARLITGSAGVSPGVSPAADGRLAQR"
BASE COUNT          757 a         1399 c         1117 g          699 t
ORIGIN      
        1 tatccgtacg tgtcgatttt gtttttggga tgtgtggccg ccgcgttctg ctcgctgtca
       61 ttgaagaatg tgctgcaggc gatcctttcg attcgcgccg tgatcccctt catggcgcag
      121 atcgtcggtg cggttttgct gcggcaacgc gaaccagatc gtccgcgccc attccgcatg
      181 tggctctacc cgctccccgc aatcatcgcc ctcggcctgt ggggctacat cgtgacgtcg
      241 ccacaaaaag gattgaaagc gggcggcctc tacgtgatgg ccgccggatc gctcttctac
      301 ctcgggcggt ggtggctgga gaagcggaaa cgcgagtctc tatgaccgag tgaaaaacca
      361 ttaccacaga gacacagaga gcacagagaa accctctgtg tcctctgtgt ctctgtggtg
      421 atcggttcta aaaacttacc tcggcatcca aacatcgctc cgcacccgga acaccgactc
      481 ctgcgcgaag ttcttcttgt acagcgcgat gatcgcggcg atgcgccctt cttcctcgcc
      541 gttcgcgcga tgcacgatct gtacgatatg cgtccgctcg tgctcgacca gaccgtgcgc
      601 gtcacgccag cggccggagg catcccacgt cgtcaatccg tccgggaaac gcgtcgtgat
      661 ctcgtcctcc aggaaacgct gccactccgt ttccgtcacc ggcgccgagc catccggctt
      721 ctgcgtcccg aagtagagcg tgtccatctc ctgctgctcg tagatcgtga ttccggagca
      781 cgagagcatc aggaggacga gcagagcgga agcggtgcgg cgcatgacga atagaatgac
      841 acgcatgcgc cgctacgtca tcgccgtcag catttttctg attaccgtca ccgcctctgc
      901 ccagctcagc gacgccgtca tcgagaagga cgttcgcgac acgatgaacg cgtggcacct
      961 gcccggcctc gccatcgccg tcgtccagaa cgatcgcgtc atcttcatca aaggctacgg
     1021 aatcaaagag atcggcaagg atgacccggt caccgccgac acgatttttg agatcggctc
     1081 gacgaccaaa gccttcaccg ccacggccat ggcgatgctt gccgatgaga agaaactctc
     1141 ctgggacgat cccgttcgca actacgtggc cggctttcac atctccgatc cctgcaccga
     1201 ttcgctgatc accctgcgcg acattgcctc gcaccgcagc ggcgtggcgc ggcacgacga
     1261 gctgtgggac gacagcgact tctcacgcga cgagctgatc aaacgcatcg gcgcgatgaa
     1321 aatctcgaag ccgatccgca ccgcatatca gtacaacaac ctgatgttcg tcaccgccgg
     1381 cgaggccgcg gcgtccgcag cgaagatgcc gtgggaggat ctcatccgca cgcgtatctt
     1441 cgccccgctc gggatgaccg aatcgcgcat cgccttcgcc gactggcccg catccgatca
     1501 cgccaccggc catcactggg acgacaaagg cgagcgcgcc gccatccaga cgatgcggaa
     1561 ctacgacgct ctcgcgcctg ccggcacgat caagtcgtcc gcacacgaca tggcgcagtg
     1621 gctccgcttc cagctcgccg gcggcgtcat cgacggcaaa cggctgctgt cagagatagc
     1681 tctcgacgag acgcacacgc cgcagacgat catcccgctc ggcacttcaa cgcgcgcgct
     1741 ctatcccgac acgaacttcg agacttacgc cctcgggtgg aacgcttccg actatcacgg
     1801 cgagctactc gttgcgcacg ccggagcgct gaacggcttc cgcaccaatg tcgcgctcct
     1861 ccccaagcgt cacctcggca tcgtgatcct cgaaaacgtc ggtcgcggct cggcgctggc
     1921 cgcgcttcgc aacaccatca tcgaccagtt cctcggcgcc ccgacgcgca actggaacag
     1981 cgacctgctc gaagtcgaga agagcgccac gttcgaagcg aacctcaaac gcggcgagcg
     2041 cgaagccaaa cgcgccaccg ctccgaagcc gtcgcacgac ctcaccgcct acgccggcac
     2101 attccacaac gccggcttcg gcgacgccac gatcgcgatc gtcaacggcg cactcgtgct
     2161 gcattgggaa cggctgacga tcccgctcgt ccacgaaaac tacgacacct tcagcgccat
     2221 cgacaccgtc gaagacatcg atgagagcgt tccattccga ctcggcagcg acggcgatgt
     2281 gaaagcattc acgctgtttg gcgaggagtt tgtaaggggt caggtctaaa gtctaaagtg
     2341 cctaactggc ctgacgcctt agcgacgatc gtccctggca ctttagactt tagacctgac
     2401 cccttcactc gcatgaacga caagcgcatc gtcatcatcg gcggcggttt tggcggcttg
     2461 tacgccgcga aagcacttcg ccgtgcgccg gtgcaaatca cgctcgtcga ccggcgcaac
     2521 tatcacctct tccagccgct gctctaccag gtcgccaccg cggcgctcaa tccgagcgac
     2581 atcgccgcgc cgattcgaag cgtccttcga aagcaggaaa acgtcggcgt gatcctcggc
     2641 gatgcgacct ccatcgatct cgatcgaaag ctcatccaac tcgccgacgg cgagcttgct
     2701 tatgacatcc tcatcatcgc caccggcgcg acgcactcgt acttcggcca cgccgaatgg
     2761 gaagaaaacg cgccgggact caagacgatc gaagacgcgc tggagatccg ccgccgcgtg
     2821 cttctcgcgt tcgaagcggc agagcgggag gacgatcccc aacgccagcg cgcctggcta
     2881 aacttcgtcg tcatcggcgg cggaccaacc ggcgtcgagc tggccggcgc gctctccgag
     2941 atcgcgcggc agacgatgct ccgcgacttt cgccgcatca atccctccag cgcccgcgtc
     3001 atcctgatcg aaggaatgga ccgcgtactc ccgccctacc cgcccgatct ctccgcgaag
     3061 gcggccgcgc aactgcaatc gctcggcgtc gaagtgatga cgaagacgac cgtcacgcgc
     3121 gtcagcgatc acgaagtcgc cgcgggcgag atgatcattc cgacgcgcac cgttctctgg
     3181 gcagccggcg ttcaggcttc gccactcgct cgttccctcg gcgccccgct cgaccgcgcc
     3241 ggccgcgtac tcgtcgcgcc cgatctcagc gtgcccgaac atccggaagt cttcgtcatc
     3301 ggcgacctcg ccgcggtgac gcaatccaac ggcaagcccg ttcccggcgt tgcacccgcc
     3361 gcgattcagg aaggagagca caccgcgaag aacatcgtac ggatgctcaa cgcccagccc
     3421 acgctcccct ttcactactt cgacaaaggc tcgctcgcca cgatcggtcg cgccgccggc
     3481 gtcgccgact tcggccgcat ccacctcagc ggcttcatcg cctgggccgc ctggctcggc
     3541 gtccacatct tcttcctcat cggcttccgc aaccgcctcc tcgtgatcct gcaatgggcc
     3601 tgggcatacg taaccttcca gcgcggagcg cggttgatca cagggagtgc gggcgtctcg
     3661 ccaggcgtct cgcccgcggc cgatgggcgt ctcgcccagc ggtaagcgcg cggcattacg
     3721 aagaacggtc ccacctcgcg caactcgcgc tgatgcgaag aatcgttcca gcttcgcgct
     3781 agacacgccg aggcgaagaa gctccggcat cgcgccacac acgccgaggc gagacgcctc
     3841 gccgcggcag gcgagacgcc tgcactcctt tcccatacaa tctcgtccac cttggcgcct
     3901 gtcgatctcg ccatcatcgt cgcctacctc gggggtgtag tcgcgatcgg gttctggtgc
     3961 cggaagcggc ag
//
LOCUS       DATLVW010000762         4524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_241505, whole genome shotgun sequence.
ACCESSION   DATLVW010000762 DATLVW010000000
VERSION     DATLVW010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241505"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VNN25_22325"
     CDS             complement(<1..430)
                     /locus_tag="VNN25_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH94330.1"
                     /translation="MPHRKLGPQGLEVSTIGLGCMGMSDFYGPSDESTNLRVLNVALD
                     IGINFLDTADVYGGGANERLLANVLKARREDIVLATKFGYVRAPDGAFLGISGTPEHV
                     HAACDASLKRLGVDHIDLYYQHRVDPKVAIEETVGAMADLI"
     gene            604..1542
                     /locus_tag="VNN25_22330"
     CDS             604..1542
                     /locus_tag="VNN25_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXH94331.1"
                     /translation="MFDSSLQRIREITGMAERQQDLSARVARLTPFDGTHQSAFPSLT
                     LTRGSVPTVCMPTVYQPCLAVVVQGRKRALLNDEVFHYDALNYLVVSVTLPAMGQVLE
                     ATPEHPYLSLRLNLDLEEIARLVLELGDRGPSPANADRGLFVARLDEPLLDAVLRMVK
                     LLDTPEDVDVLAPVVRREIYYRVLRGELGSRLADLAQAEGGKHRIVRAIEWLKQHYAA
                     PLRIEELAEAVHMSPSALHHRFKAVTAMSPLQYQKHLRLHEARRLMFADGIESATAGH
                     RVGYESASQFSREYRRLFGAPPRSEIARLRDTGVSA"
     gene            complement(1562..2425)
                     /locus_tag="VNN25_22335"
     CDS             complement(1562..2425)
                     /locus_tag="VNN25_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94332.1"
                     /translation="MRYIALAIVVTALGISIPAHAQQPGPIAAVGDENKENADSFFPL
                     WKSLLKDGKFLPPYGINLVFFDLNGLWDVKNFSASVNGNEVASASGKAEVHPFTYGGR
                     ADVWVFPFLNVFATAGGLNLKVQVTGLDLPLGISGDRPPEVIRGDVHFDLDFTGYYGG
                     GGFVLMYAWRSYFASADYSTVWTHLESSTSGVTGNELRTDTASFRVGYNAGAVQPYIG
                     ARWVKKIDHFEGTVSTSGGLPLTFAVDLQAPAWNYQVGIHSIIARRYELVVEAGFGDR
                     THGLVNLGYRF"
     gene            complement(2467..3186)
                     /locus_tag="VNN25_22340"
     CDS             complement(2467..3186)
                     /locus_tag="VNN25_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94333.1"
                     /translation="MATFLALSLSTSASVGPLAGAMAACTAQGRLLAALRSKIPLEPI
                     AAKGDPYMIWHTQEPPRAPAGTLCGVRFEPDQVHYHLSTFSTPAAARTAGFSVTHFGA
                     CGTCSTLQDLAVYLEKPDLTSPVRRCGISWDASASLTCLEDLGFSAACAQTWLYNVNN
                     TRRHCFSVCIVAWITSEAPTRKDGRLNDCLQCDEDRSGPVFKATAGRTRRNSGIQSSI
                     PRPNEEIAPVVHDYVPDIAPC"
     gene            complement(3195..3614)
                     /locus_tag="VNN25_22345"
     CDS             complement(3195..3614)
                     /locus_tag="VNN25_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94334.1"
                     /translation="MMVPPRQRGQILILLAGWLFFGGGASSALVVYDRPAKEMKKVIK
                     RVITDADRRDVILSDLSRWKSGQKKLDKEASSDRKELLKTLRRKDAERSEAEPIMAKL
                     DETFLKMDRNFLDLRFRVKEQVSSAEWAEIVARPNRE"
     gene            complement(3611..4036)
                     /locus_tag="VNN25_22350"
     CDS             complement(3611..4036)
                     /locus_tag="VNN25_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94335.1"
                     /translation="MALTGPGCSSTKPTSGQTINVYAEKLREAVYANVPEHGPRAQML
                     SIVDQVESVHLRFSRETANFIESYRTLNADYDSTRPAFDQLFSDYNAKRIKARSEALD
                     LHFQLASLATAGEWEPIGRAEKKLYEEASAALPTQESTK"
     gene            complement(4213..>4524)
                     /locus_tag="VNN25_22355"
     CDS             complement(4213..>4524)
                     /locus_tag="VNN25_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HXH94336.1"
                     /translation="FLEKNLIERTRKAPAVWDMIVYVGEPGDPQDNPTLAWPETRKHF
                     TAGTLTITQAAPQQKGVACEPINFDPLVMADGIAPTNDPVLLFRSPAYAVSYAKRLSG
                     Q"
BASE COUNT          866 a         1339 c         1408 g          911 t
ORIGIN      
        1 tgatcagatc cgccatggca cccactgttt cctcaatagc gaccttcggg tcgacgcggt
       61 gttgatagta caaatcgatg tgatcgacgc cgagtcgctt gagactggca tcgcaggcgg
      121 cgtgcacatg ctctggtgtt ccgctgatgc cgagaaacgc gccatcggga gcgcgtacat
      181 agccgaactt ggtggcgaga acaatgtcct cgcggcgcgc ttttaaaacg ttcgccagca
      241 ggcgttcgtt ggcaccccct ccgtacacat ctgcggtgtc taggaaattg atgccgatat
      301 cgagcgcgac attgagcaca cgcagattgg tgctttcgtc ggacggacca tagaagtccg
      361 acatgcccat gcagccgagg ccgatggtcg agacttccag accctgtggg ccgagtttgc
      421 gatgcggaat tttcatgagg ttctcccggg cggttgggaa ttcatgctag acatcggcgt
      481 attgggatgc tagatcatta ctgccgggtt cttgcctaat cctgcaaagg gcgtgatcct
      541 cagtttcgct gagcgtagcg accggctacc atgccgcaac cgctcgagga ttgagaaaca
      601 ctcatgtttg acagcagttt gcagagaatt cgcgagatca ccggcatggc cgaacggcaa
      661 caagatctgt cggcacgggt tgcccgattg acgcctttcg acgggaccca tcaatcggcc
      721 tttccgtcgc tgacgttgac ccgcggcagc gtgcccacgg tgtgcatgcc gacggtttac
      781 cagccttgtt tagccgtcgt cgtgcagggt cgcaagcgtg cgctgctgaa cgacgaagta
      841 ttccactacg acgcactcaa ctatctcgtg gtgtcggtga ccttgccggc gatgggccaa
      901 gtgctcgaag ccactccgga gcatccatac ctatccctgc gtctgaatct cgacctggaa
      961 gaaatcgcgc gcttggtgct ggaattgggc gatcggggac cctcgccggc gaacgccgac
     1021 cgcggcttat tcgtcgcgcg cttggacgag cctttgctgg acgccgtatt gaggatggtg
     1081 aagctgctcg acacgccgga ggatgtcgac gtgctcgcgc cggtggtacg gcgtgaaatc
     1141 tactaccgcg tgttgcgtgg cgaactgggg tcccgcctgg ccgatctcgc ccaggccgag
     1201 ggcggcaagc atcgcatcgt tcgcgccatt gagtggctca aacaacacta tgcggcgccg
     1261 ttgcgcatcg aggaactggc tgaggccgtg catatgagtc cctcggcact gcatcaccgc
     1321 ttcaaggcgg tcaccgcaat gtcgccgctg cagtaccaga aacacctgag actgcacgag
     1381 gcccggcggc tgatgttcgc cgacggcatc gaatccgcta ccgcggggca ccgagtgggc
     1441 tacgaaagtg cttcgcagtt cagccgcgaa taccgccgcc tgttcggcgc cccgccgcgg
     1501 tcggagatcg cgcgcttgcg cgacacggga gtgtcggcat agcgcgactg agcccgcctt
     1561 tctaaaaccg gtagccgaga ttcacgaggc cgtgtgttct atcgccgaac ccggcttcca
     1621 cgacgagttc gtagcggcgc gcaatgatcg aatggatccc gacctggtag ttccacgccg
     1681 gcgcctgaag atcgactgcg aatgtcagcg gcaggccgcc ggacgtgctc acggttccct
     1741 cgaagtgatc gatcttcttc acccaacggg cgccgatata tggctggacg gccccggcgt
     1801 tgtagcccac tcggaaggat gcggtatccg tccgtagctc gtttcccgtg actccggacg
     1861 tcgaggattc gaggtgggtc cagacggtgg agtagtcggc cgaggcaaag tagcttcgcc
     1921 aggcgtacat gagaacaaag ccaccaccgc catagtagcc ggtgaagtca aggtcgaagt
     1981 ggacgtcccc gcgaatcacc tctggcggac ggtcgccgct gattccgagc ggtaaatcga
     2041 gaccggtaac ttggaccttg agattcagcc cgccggcggt cgcgaatacg ttcaaaaatg
     2101 ggaacaccca gacgtcggca cggcctccgt aggtgaatgg atgaacttcc gccttgccgc
     2161 tcgccgaggc gacctcgttg ccattgaccg acgcagagaa attcttgacg tcccatagac
     2221 cgttgagatc gaagaaaacg aggttgattc cgtacggcgg aaggaacttc ccgtctttga
     2281 gaagcgattt ccagagtgga aagaaggagt ccgcgttctc cttgttttcg tctccgacgg
     2341 cggcgatcgg gccgggttgc tgggcgtggg cgggaatcga gatgcccaat gcggtgacaa
     2401 cgattgcgag agcgatataa cgcaagagac tctccttgag gcagtgggta ctcattgaga
     2461 tcggccctag cacggtgcaa tgtccggaac gtagtcgtgg acgaccggtg caatctcctc
     2521 gttcgggcgc gggatcgacg attggatccc tgagttgcgg cgggtccgcc cggcagtggc
     2581 cttgaagacc gggccggagc gatcttcgtc gcactgcagg cagtcattca gccgaccgtc
     2641 ttttcgtgtc ggcgcctcac tcgtgatcca ggccactatg cagacgctga agcaatggcg
     2701 gcgggtgtta ttgacgttgt acagccaggt ctgcgcgcac gcagcgctga agccgagatc
     2761 ctcgaggcac gtcaggctgg cggacgcgtc ccagctgatt ccgcacctgc gcacaggaga
     2821 cgtaaggtcc ggcttctcga ggtaaaccgc caggtcttgc agtgtcgagc aggtcccaca
     2881 ggcgccaaag tgggtgacgg aaaacccggc tgtgcgcgcc gccgccgggg tcgagaatgt
     2941 ggagagatgg tagtggactt gatccggctc gaaccgcaca ccgcaaagcg tgccggcggg
     3001 cgcgcggggc ggctcttggg tgtgccaaat catgtaggga tcgcccttgg ccgcgatggg
     3061 ttcgagtgga atcttggaac gaagcgcagc cagaagacgc ccctgggcag tgcaggcggc
     3121 catcgccccc gcgagcggcc cgaccgatgc cgaggtcgac aggctcaggg caagaaatgt
     3181 tgccatagcg aacttcactc gcggttgggc cgcgccacga tctcggccca ctccgcgctg
     3241 ctgacctgtt ctttcacccg gaaccgcaaa tcgagaaagt ttcgatccat cttaagaaac
     3301 gtttcgtcca gcttcgccat gatgggttcc gcctcggagc gctccgcgtc cttgcgtcgc
     3361 agggttttga ggagttcctt acggtccgaa ctcgcttcct tgtctagctt cttttggccg
     3421 gacttccagc ggctaagatc ggacagaatc acatccctgc gatccgcatc cgtgatcaca
     3481 cgcttgatca ctttcttcat ctccttggct ggacggtcgt agacgaccag cgcgcttgaa
     3541 gcgcccccgc cgaagaacag ccatccggct aaaaggatga gtatctgacc gcgctgtctg
     3601 ggagggacca tcatttcgtg ctctcctgtg tcgggagcgc cgcgctggcc tcctcataca
     3661 actttttttc agctcttccg ataggctccc actcacccgc tgtggccagc gacgccagct
     3721 ggaagtgcag atcaagagct tcactgcgcg ccttgatgcg tttcgcgttg tagtcggaga
     3781 acagttgatc gaacgccggc cgcgtggagt cgtagtcagc gttcagggtg cgatagcttt
     3841 cgatgaaatt tgccgtctca cgactgaagc gaaggtgcac cgactccact tggtcgacaa
     3901 tggagagcat ctgagctctt ggtccgtgct cagggacgtt ggcgtagacc gcctcacgca
     3961 gtttctcggc gtagacattg atcgtctgac cgctggtcgg cttcgtactg ctacatccgg
     4021 gacctgtcag cgccacggcg cataccaaga gcgcaaccag agcggcgaaa ccggaactta
     4081 tttcatgtcg caccctggct cccccgatcg tcgccgaatg cgacgctctg ggggtgttat
     4141 agcgccaggt aggccgggac ttaaatgaga cctatgacct agatggcggt gccgatcggg
     4201 cagccggccg tctcactggc cggacaagcg tttcgcgtac gagaccgcat acgccggtga
     4261 gcggaacaac aatactgggt cgttggtggg agcgatgcca tccgccatca ccaatgggtc
     4321 gaagttgatc ggctcacagg ccacgccttt ctgttgcggc gctgcctggg taatggtgag
     4381 agtaccggcg gtgaaatgct tgcgcgtctc cggccacgcc aatgtcgggt tgtcctgcgg
     4441 gtcgccgggc tcgccgacgt agacgatcat gtcccagacc gccggcgcct tgcgggtgcg
     4501 ctcgataaga ttcttctcaa ggaa
//
LOCUS       DATLVW010000763         4710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_366214, whole genome shotgun sequence.
ACCESSION   DATLVW010000763 DATLVW010000000
VERSION     DATLVW010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366214"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1364)
                     /locus_tag="VNN25_22360"
     CDS             complement(366..1364)
                     /locus_tag="VNN25_22360"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HXH94337.1"
                     /translation="MYEVTPGDASVDVRCSSASEFLHLLEPIRGLFASPTFIFRGVSS
                     DRHGLLPSAHRPKAPLLTPNNVVVSGPLDSLRTQCGAEFYTIDRFFKIASRHGVRMPE
                     DSYVLREELDEWSVLFQQNEAAPYHGRVWPSPRLFSLIALAQHYAIPTRALDWTYSAY
                     VAAYFAARPALNNDADSICVWAVDDFHRQVDRVLAAAAKRPLRVFTVSGAENENLRAQ
                     RGLFMLHPQSLDDPWARFLPTTYNALLLESLPLLKDAVQFIRVLLDGSHAREVLGLLA
                     TAGITRGALYPGLSGVAQEFEDEQLMPPRRAAPGGDTHETIGLWDKINKAARRIRT"
     gene            complement(1419..1913)
                     /locus_tag="VNN25_22365"
     CDS             complement(1419..1913)
                     /locus_tag="VNN25_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94338.1"
                     /translation="MAFIIDPTAADFLSKEATFVYDLYHAAELEVRLTERYVLLALGG
                     IYSYLATTAAKEIPIRFRRLAWYAPTFVVVFAGFRALGLGFRQKEITEWLLRAEYSAH
                     IAKPAGWAQSTVCGFPIMAATASVFYIVLTVTTGFLAHRMSRSDSDFRRRRRRAIRWR
                     GAST"
     gene            complement(2032..3183)
                     /locus_tag="VNN25_22370"
     CDS             complement(2032..3183)
                     /locus_tag="VNN25_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HXH94339.1"
                     /translation="MNDTVDFNVSPFVVIWENTRACDLACVHCRAAAQPKRSQFELTT
                     EEGFALIDEVAVMKPRVFVITGGDPLKREDTYDMIRYAKDLGLEPALTPSATPLLTEG
                     AVARMKNHGLSRLAVSLDASRADMHDAFRRVDGSFDITIRAVEAARKEGIPVQINTTV
                     TRRTIDDLPNMVTLLTDLGVAMWSVFFLVPTGRGKRGDLVTAETVEHLFSFLYETTKR
                     APFAIRTTEAMHYRRYMLQRMALENGRRIDDLRDRETGLIDASTLFLGHGARPMPLGV
                     KAQHAATSRAPRGVNEGKGFVFISHIGDVFPSGFLPLKAGNVKTESLSQIYRTSDLFV
                     SLRNTSNLKGKCGRCEFRDLCGGSRARAWSMTGDVFEEDPLCGYQPKVA"
     gene            complement(3180..4352)
                     /locus_tag="VNN25_22375"
     CDS             complement(3180..4352)
                     /locus_tag="VNN25_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXH94340.1"
                     /translation="MIGLEAEVAAPDVASLADDAPAGPSISTSIRATDPINRRILEVS
                     EDRIRGFVSDPFSNIAELSGVPLPVVTQRIRAMLEAGTIRRVRQTLLATNLAQGALVA
                     WKIDEELLEAAFAFIAKRDPFSGHVVIRNGENGPTEWRLWTTIKVPSEFSLEGHCEYL
                     RRRIGAHKFRIMPTLRAFVLGVGHMRRKGMAIGAMSPQPAAATQPAVVELSEPEWLIL
                     SALKADLAPAEIGPGLWSRRALAAGIHPATFLEIASELDRRGVIGRFSTFLEHTKPVA
                     GAERVSSFNGLFHWAVPAGREIEAGCEIGRFEILTHCYWRDAGPELNNVNIMAVAHGE
                     TKESVMAHKSAIDAHLNAIGIGFTHTNVYWGGRAEIKPSEILPEAYRDWARSTGLL"
     gene            complement(4349..>4710)
                     /locus_tag="VNN25_22380"
     CDS             complement(4349..>4710)
                     /locus_tag="VNN25_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXH94341.1"
                     /translation="RADEALSLMRNNGGNRSGIAIAAIDSFGIVHPDQFTSTHAVGSI
                     RERPFSQIWNDSRIQLLSNLRDRKPLLQGRCATCQWLPCCNGNFRARAEAATGDYWQS
                     DPGCYLLDSEVISGAPE"
BASE COUNT          912 a         1400 c         1504 g          894 t
ORIGIN      
        1 cttcccagca gcgggctccg agccagcctt ctctttttcg gctagctgtt gttcgagctg
       61 cgcaacaaga cgccgcaccc ggcgcctgta gctgaacttg aagtacagcg ccaccgccgc
      121 agcgacgatc gacagcaaat agatcagatc caccatcagc ctgggaagat atcaaggagt
      181 gcaggcgtct cgcctgcggc cggcgggcgt ctcgcccggc ggtgtggaac gcgcaagagt
      241 gacgagtcgc gacagagccc tgacacacac gccgaggcga ggacgcctcg acgccgcggg
      301 cgagacgccc tgcactcctc tcgtatcatc cgggcgagac gcggaaccca tcgaccgtga
      361 aggagctatg ttctgattcg tctagctgct ttgttaattt tgtcccacag gccgattgtc
      421 tcgtgcgtat cacctcccgg agcagcgcgc cgtggcggca tcaactgttc gtcctcaaat
      481 tcttgcgcca ctccggaaag gcccggataa agcgcgccgc gagttatgcc tgcggttgcg
      541 agcagaccca gtacttcgcg agcgtgagaa ccatccagaa gaacgcgtat gaattgaacg
      601 gcgtccttca gaagcggtaa actctcaagg agtagtgcgt tgtaggttgt cggcaggaac
      661 cgcgcccacg ggtcgtcgag actctgaggg tggagcatga acaaacctcg ctgagcgcgc
      721 aggttttcgt tctcagctcc tgaaactgta aagacccgaa gaggccgttt cgccgcggca
      781 gcaagcacgc gatctacttg tcggtgaaag tcgtccaccg cccaaacgca gatggagtcg
      841 gcgtcgttgt tcaatgccgg tcgtgccgcg aagtaagcgg caacatatgc gctataggtc
      901 caatccaacg cgcgtgtagg gatagcgtag tgttgcgcta gtgcgatcag cgagaagagc
      961 cgtggcgaag gccacactcg cccgtgataa ggcgcagcct cgttctgctg aaaaagaacg
     1021 ctccactcat cgagctcttc gcggaggaca tacgagtcct cgggcatgcg gacgccatgg
     1081 cgcgacgcga tcttgaaaaa gcggtcgatc gtgtaaaact cagcgccaca ctgtgtgcgg
     1141 agtgaatcca gtggtccaga cacaacgacg ttgttgggcg taaggagggg agccttgggc
     1201 ctgtgggcgg aaggcagcag cccgtggcgg tcggacgata caccgcggaa gataaatgtc
     1261 ggtgatgcaa acagcccacg aatcggttcg agcaggtgca gaaattccga cgccgaggaa
     1321 cagcgcacgt caacggatgc gtcccccggc gtgacttcgt acatttgctg agtttatcgt
     1381 tagttaggtc cgctggcatg tgcgcccaac gaccttccct acgtcgaagc gccgcgccaa
     1441 cgaatcgcac gccgccgccg acgacgaaaa tcactgtcgg atcgactcat tcgatgagcc
     1501 aggaaacccg tggtcaccgt gagtacgatg tagaagacgc tcgcagtggc agccatgatc
     1561 gggaacccgc agacggtcga ttgtgcccag cctgctggtt ttgcaatgtg cgctgagtac
     1621 tcggctctca agagccactc cgttatctcc ttttgtcgaa atccaaggcc tagcgcgcga
     1681 aaaccggcga aaacgacgac aaatgtgggt gcgtaccaag cgagccggcg aaagcggatc
     1741 ggaatctctt tggcagcggt cgtggcgagg tacgaataaa tgccaccaag tgccaaaaga
     1801 acataacgct ccgttaagcg cacttccaac tccgcagcat gatataggtc gtatacaaac
     1861 gtcgcctctt tgcttaggaa atctgccgcg gttggatcga tgatgaatgc catacccgcc
     1921 tccggaaggc ctatggtatg gctacacgac gtccgtgtat acgaagttcg ctcctacctc
     1981 ggggcgtttt tccgcgagta acgatcgacc cacggcgaag agcgagtgac tctacgccac
     2041 cttcggctga tacccgcaca gcggatcttc ttcgaacaca tcccctgtca ttgaccaggc
     2101 tcgtgctcgc gacccgccgc aaagatcgcg gaactcgcag cggccgcatt tgcctttcag
     2161 gttgctcgtg ttgcggaggg agacgaagag gtcgctcgtg cggtagatct ggctgagcga
     2221 ctccgtcttc acgttgccgg ctttcaaagg gaggaagccg ctggggaaga cgtcgccgat
     2281 gtgggagatg aagacgaagc ctttgccttc gttgacgccg cggggggcgc gcgaggtggc
     2341 ggcgtgttgt gctttgacgc cgaggggcat ggggcgggcg ccgtgtccga ggaagagggt
     2401 ggaggcgtcg atcaggccgg tctcgcggtc gcgcagatcg tcgatgcggc ggccgttttc
     2461 cagcgccatt cgctgcagca tgtagcggcg atagtgcatc gcttccgtcg tgcggatcgc
     2521 gaagggcgcg cgttttgtcg tctcgtaaag aaagctgaag agatgctcga ccgtctctgc
     2581 ggtgacgaga tcgcctcgct tgccgcgtcc ggtcggaacc aggaagaaga cgctccacat
     2641 ggcgacgccg aggtcggtga gcagcgtgac catgttggga agatcgtcga tcgtgcgccg
     2701 cgtgaccgtc gtgttgatct gaacgggaat cccttctttt cttgcagcct cgaccgcgcg
     2761 gatcgtgatg tcgaatgagc cgtcgacgcg gcggaaggcg tcatgcatat cggcgcgcga
     2821 ggcatcgaga ctgacggcga gccgcgagag gccgtggttc ttcatgcgcg ccacggcacc
     2881 ctcggtgagc agcggtgtcg cacttggcgt cagcgccggc tcgaggccga ggtccttcgc
     2941 gtagcggatc atgtcgtacg tgtcttcgcg cttgagcgga tcgccgccgg tgatgacgaa
     3001 gacgcgcggt ttcatcaccg ccacttcatc gatgagcgcg aagccttcct cggtcgtgag
     3061 ctcgaactgg ctccgcttcg gttgcgcggc ggcgcggcag tgcacgcatg caagatcgca
     3121 ggcgcgcgtg ttttcccaga tgaccacaaa cggactgacg ttgaaatcga ctgtatcgtt
     3181 catagaagtc ccgtgctccg tgcccaatcg cgatacgcct cgggcagaat ctccgacggc
     3241 ttgatttcgg cgcggcctcc ccaatacacg ttcgtgtggg tgaaaccgat gccgatggcg
     3301 ttgagatgcg cgtcgatcgc gctcttgtgc gccatcacgc tttctttcgt ttcgccgtgc
     3361 gcgacggcca tgatgttgac gttgttcagt tccggtccgg catcgcgcca gtagcagtgc
     3421 gtcaggatct cgaagcggcc gatctcgcat cccgcttcga tctcgcgtcc cgcgggcacg
     3481 gcccagtgaa agaggccgtt gaagctcgag acgcgctcgg ctccggccac cggtttggtg
     3541 tgctcgagaa atgtggagaa gcgtccgatc acgccgcggc gatcgagctc gctggcgatc
     3601 tcgaggaagg ttgctggatg aatccccgct gccagcgccc gccgcgacca caatcccggt
     3661 ccgatctccg ccggcgcaag atcagctttg agcgctgaga ggatgagcca ttcgggctcg
     3721 gaaagctcga cgaccgcagg ctgcgtcgct gccgcgggct gcggggacat tgcgccgatc
     3781 gccatccctt tgcggcgcat gtggccgacg ccgaggacga aggcgcgaag agtgggcatg
     3841 atgcgaaact tgtgagcgcc gatcctgcgg cggagatact cgcagtgtcc ctccagcgaa
     3901 aactccgaag gaactttgat cgtcgtccag agccgccact ccgtcggtcc gttctcgccg
     3961 ttgcggatga cgacgtggcc ggagaatgga tcgcgtttgg cgatgaatgc gaaggctgcc
     4021 tcgagcagct cctcatcgat cttccaggcc acgagcgcgc cctgcgcgag gttcgtcgcc
     4081 agcagcgtct gccgcacgcg acgaatcgtt cccgcttcga gcatcgcccg aatgcgttgc
     4141 gtgacgacag ggagcggcac gcctgacagc tcggcgatgt tgctgaacgg atcgctcacg
     4201 aatccgcgga tgcgatcttc cgacacttcg aggatgcggc gattgatcgg atcggtcgcg
     4261 cgaatcgacg tgctgatgga cgggccggcc ggcgcgtcgt cagccagcga agcgacgtcc
     4321 ggtgctgcca cctcggcttc gagcccgatc attccggcgc ccccgagatg acttctgaat
     4381 cgagcaggta acatcccgga tcggactgcc agtaatcgcc ggtggccgct tcggcgcgcg
     4441 cgcggaagtt gccgttgcaa cacggcagcc actggcacgt cgcgcagcgt ccttgcagca
     4501 aaggcttgcg atcacgcaaa ttgctcagaa gctggatccg tgagtcgttc cagatttgcg
     4561 agaacggccg ctcgcgaatc gatccgaccg catgcgtcga ggtgaactga tcgggatgaa
     4621 cgatgccgaa gctgtcgatg gctgcgatgg cgatgccgga gcggttgccg ccgttgttgc
     4681 gcatcagcga gagcgcttcg tccgcgcgca
//
LOCUS       DATLVW010000764         7304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_366244, whole genome shotgun sequence.
ACCESSION   DATLVW010000764 DATLVW010000000
VERSION     DATLVW010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366244"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VNN25_22385"
     CDS             <1..375
                     /locus_tag="VNN25_22385"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXH94342.1"
                     /translation="QPDSPHLPHHYEPQTVVYTGTHDNDTARGWYDQATDEERTTAAL
                     YLGVHTSEELPWTLIRTAYTSVADTAIVPAQDILGLGNEARMNLPGEEKDNWSWRLEE
                     GALTEELADRLRKLAEVSGRTE"
     gene            complement(597..1148)
                     /locus_tag="VNN25_22390"
     CDS             complement(597..1148)
                     /locus_tag="VNN25_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH94343.1"
                     /translation="MAPETSTKMTYEDLLLIPEDGLRHEIIDGEHYVNPSPITRHQRV
                     SMRLILEISVYLREHPMGELFHAPLDVVFSRYDVVEPDLIFISNERREIITTKNIQGS
                     PDLLIEILSESNRKYDEVTKRALYERTGVGEYWIVDPSANTVRAFRNEAGQYEPAAEL
                     SRNDLLTSPLFPNFEIRLARIFA"
     gene            complement(1769..4474)
                     /gene="treY"
                     /locus_tag="VNN25_22395"
     CDS             complement(1769..4474)
                     /gene="treY"
                     /locus_tag="VNN25_22395"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXH94344.1"
                     /translation="MYRVPRATYRIQFNAGFTFADAQRIALYLEELGISDVYASPILR
                     ARKGSSHGYDVVDANSLNPELGSDDDFDALHQELERRKLGLLLDIVPNHMAASAENAW
                     WMSVLENGEHSRFLHYFDIDLTPVTTRGHTAAKVLLPILGKPYGEVLEAQEIQLHFDA
                     DGFFFTYYDKRLPLSPDSYSLVLRACLEFLPENSVSIELRELTENHDSVPNSRFLKDA
                     LWRIHEQDEAFRDALHSAIARINGKQGKPDSFNALDALLDAQSYRLAYWRIASEKINY
                     RRFFDVTDLVGVRVENPEVFEARNRRTLELIAEGKVTGLRIDHIDGLYDPIGHMRKLQ
                     SRLADADGRFYILLEKILEDGEELRSEFPVSGTTGYDFLASVNVLFVDADGLQQLGRF
                     YRSVTGVNETFADVCYQRKRQVIHELFSGEMRALGKQLGALALTDRNARDFAPVDLTA
                     ALTEVTACMRVYRTYLRNGEVDPADQQSIREAIAEARRRSGTAIDERLFTFLGSVLLV
                     DPPPYVASEREHWLSFVMRWQQFTGRVMAKGVEDTAFYNYNRLISLNEVGADPGRSAE
                     ADALAEFHTRNEHIQRNWPHTMNATATHDTKRGEDVRARISVLSEMAPQWEHQVRRWS
                     KMNAARKKNGVPHANEELLLYQTLIGIWSLDERELPSLPDRLQQYLEKASREAKNHTS
                     WIAPNRQYEEALLGFAGAILSDQEFRSDFLRFQKRIAFYGFLNSLSQVVLKATAPGAP
                     DFYQGTELWDFSLVDPDNRRAVDYGRRRSMLKEVKSASPDTLLNRWEDGRIKLYVTWK
                     LLELRTRHADLFRDGSYEPIEAGPNVVAFIRRHNNDAVLIAVPRLIANLVKPGRLPIG
                     DVWGDATLTAPGKWRNVFTGEERKGDEIALSCLFSTLPIAALESA"
     gene            complement(5255..6787)
                     /locus_tag="VNN25_22400"
     CDS             complement(5255..6787)
                     /locus_tag="VNN25_22400"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH94345.1"
                     /translation="MLDEDRRRAERLLLPAPIPARVAESVVRIVDIGILGTRIEHEEP
                     LMAGPHTLQFDWDGEQIEVDCTIVRSERSETVFQSGLQFSERDRILVGRIVTTLADRD
                     EMERLRTLVEASKLINSSIEPNALFASILTVARNELHVERGTLYFVDDQKKEIWTKIA
                     GELATEIRLPIGKGLAGKVAETGEAVILHDAYQDPRFDRSLDQKTGYRTRSMLCVPIK
                     NRGERVVGVLQLLNKIKGSFGPRDLEFLNDISDHMAIAMENATLHMELLEKQRMEQEL
                     ALGREIQSRLLPSAPVDVKGIELGALSLPCYEVGGDYFDFLELPGGDLGLAIGDVSGK
                     GVGAALIMSSVQAALRVAAPIEDDLARLVARLNALIFRSARGRKYATFFFARYSPATG
                     VLRYVNAGHNPPYIATAEGLTEIGSTGRPIGILPESSYTEGTIELPHGSVLFLYTDGL
                     NEAADPDDVEFGNDNLRELFLAQRNEPTNDIPSRVLDAVIAFERGAKATDDKTIVVMR
                     RV"
     gene            6962..>7304
                     /locus_tag="VNN25_22405"
     CDS             6962..>7304
                     /locus_tag="VNN25_22405"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH94346.1"
                     /translation="MFEAFQESDYRRFWVAQFISNIGTWMQGLAQGWLVYKLTESPFL
                     LGFVAFANSAPSLILMLPAGVLADHLDRRRVVGLSQIAQALAALFVAISIRTGHITVW
                     HIVAASAVSGVA"
BASE COUNT         1268 a         2134 c         2408 g         1494 t
ORIGIN      
        1 cagccggaca gcccgcattt gccacatcac tatgagcctc aaaccgtcgt ttacaccggc
       61 acgcacgaca acgacaccgc ccgcggctgg tacgaccaag ccaccgacga ggagcgcacg
      121 accgccgcgc tctacctcgg cgtgcacaca tccgaagagc tcccctggac cctgatccgc
      181 accgcgtaca cttccgtggc cgacacagcc atcgtcccgg cgcaggacat cctcggccta
      241 ggcaacgaag cccgcatgaa cctccccggc gaagagaaag acaactggtc gtggcggctg
      301 gaggaaggcg cgctcacgga ggaactggcg gaccggctgc gaaaattggc agaggtttcg
      361 ggcagaacgg agtaatcgcg actgccggag gagtgcggtc gtctcgaccg cgggcactcg
      421 ggcgtccccg cccgggtgct tggcctcggt tcctgatgag cccttagcgt gctcctccgc
      481 ttcctgcatg ctccggcaat tgggcgagaa ttggtgttat gggctcttgg cccgaggcca
      541 accaccgagg ggggacgccc cgctggccgc gcgcgagacg cacgcactcc tttggctcag
      601 gcaaagatgc gcgcgagtct gatctcgaaa ttcggaaaaa gcggtgaggt gaggaggtcg
      661 ttgcgagaca actctgcggc gggttcgtac tggcctgcct cattccgaaa ggcgcgaacg
      721 gtgtttgcgg acggatcgac gatccagtat tcgccgacac ccgtgcgctc gtagagagcc
      781 cgcttggtga cttcgtcgta cttgcggttc gattcggaga ggatctcgat gagaagatcg
      841 ggcgaaccct ggatgttctt cgtggtgatg atctctcggc gctcgttgct gatgaagatc
      901 agatcaggtt cgacgacgtc gtatcgtgag aacaccacat cgaggggtgc gtggaagagc
      961 tcacccatcg gatgctccct gaggtaaacg ctgatctcga ggatgaggcg catggagact
     1021 cgttgatgcc gggtgattgg cgatgggttc acgtaatgct ccccgtcgat gatctcgtgg
     1081 cggagaccgt cctcggggat gaggagtaaa tcctcatagg tcatcttcgt cgatgtctcg
     1141 ggcgccatgt tcgtagttta cgccttcacg ctctcgcgag ggttcgctag atgcggattt
     1201 tctcaagtgg aaattgatcg ccgtggcaac ggtggcgtcc gttgcggtcg gcttcttggc
     1261 gtccaccaag gaggctccga ggcccgacat acgtctgatc atttgcctag ccccgctaat
     1321 ttgtgcgtac gccgatatgg tcggtgtcga tctggcggtc agaacaatgc tgatcgctaa
     1381 cttcttgtgg cggtgcggcg acccgtatga aactcatgtt cacaaccttc gcgagcgtgg
     1441 caggcgcccg gctgcacttg gtaagcgtag aaaaggtcca ttcaacgccg ctacgacggc
     1501 gtcttttgcc gcgagccttg cggtcagtac gcttattata ggtggtggac ttgtgggcgg
     1561 tttcaagacg actccaccgt ggccacttcc acatgttgtc gcgtttgtcg cagcgggtgc
     1621 attgggcgta gcagcaaccc tgtacctgtg ggctctgtac gccgcaaggc tggaccaaac
     1681 agctgtgcgt tccattgagc cgacggacga cgcgctaacg ccttgaagga tccgccgcgc
     1741 ttagaagcct cgtgctggaa tccaagaact acgcgctctc cagggcggca atcgggagcg
     1801 tcgagaacaa acagcttaga gcgatctcgt ctcctttgcg ctcctcgccc gtgaagacat
     1861 tcctccactt gccgggggcg gttagagttg catcccccca tacatcgccg atagggagcc
     1921 tgcctggctt tacgaggttg gcaatcaagc ggggtacggc gatcagcacc gcgtcgttgt
     1981 tgtgacgacg gatgaacgcg acgacgttcg ggccggcttc gatcggttcg taactgccgt
     2041 cgcggaagag atcggcgtgg cgggtgcgta gctccagcag tttccaggtg acgtacagct
     2101 tgatccggcc atcttcccag cgattcagga gcgtgtcggg agaggcggac ttgacctctt
     2161 tcagcatcga gcggcggcgc ccgtagtcga cggcgcgccg gttgtcggga tcgacgaggc
     2221 tgaagtccca cagttctgtt ccctggtaga aatcgggcgc acccggcgca gtcgctttca
     2281 gtacgacctg cgacaggcta ttgaggaatc cgtagaaggc gatgcgtttc tggaagcgga
     2341 ggaagtccga tcggaactcc tgatcgctga ggatggcccc tgcaaatccg aggagtgcct
     2401 cttcgtactg tcggttcgga gcgatccagc tcgtgtggtt cttcgcctcg cgtgaggctt
     2461 tttcgagata ttgctgcaga cgatcaggca gggacggcag ctcgcgctca tcgagagacc
     2521 agatcccgat cagcgtctgg tagagaagta gctcttcgtt ggcgtgaggg acgccgttct
     2581 tcttgcgggc ggcattcatt ttcgaccagc ggcggacctg gtgctcccat tgcggcgcca
     2641 tttcggagag cacactgatg cgtgctcgga cgtcttcgcc gcgcttcgta tcgtgcgtcg
     2701 cagtggcgtt catcgtgtgc ggccagttgc gctgaatatg ttcgttgcgc gtgtgaaatt
     2761 cagcgagagc atcggcctct gcgctgcggc caggatcggc gcccacttca ttaagagaga
     2821 tcaaccggtt gtagttatag aaagcggtgt cctcgactcc cttggccatc acgcggccgg
     2881 tgaactgctg ccagcgcatg acgaacgaca gccaatgctc gcgctcggag gcgacatagg
     2941 gtggaggatc gacgaggagg acgcttccga ggaaggtgaa gagcctttca tcgattgctg
     3001 tgccggatcg acggcgcgcc tcggcgatgg cctcacgaat cgactgttga tcggctgggt
     3061 ccacctcccc attgcggagg tatgtgcggt aaacgcgcat acaggccgtc acttcggtca
     3121 gcgcggcggt aaggtcgacg ggcgcaaagt cgcgggcgtt tcggtccgtg agcgccaacg
     3181 cgcccagttg cttgccgagc gcgcgcatct cgccggagaa aagctcgtgg atgacctgac
     3241 gcttgcgctg gtagcagaca tcggcaaagg tctcattgac accagtaacg gatcgataga
     3301 accgcccgag ctgctgcaat ccatcagcat cgacgaagag aacattcacc gacgccagga
     3361 agtcatagcc ggtggtcccg ctgactggaa actcggagcg cagctcctcg ccatcctcca
     3421 ggatcttctc gagaagaatg taaaagcgac cgtcggcgtc agcgaggcgc gattgcagct
     3481 tgcgcatatg tccgatcggg tcatagaggc cgtcgatgtg atcgatgcgc aggccggtga
     3541 ctttgccttc cgcgatcagc tccagcgttc gccgattcct cgcttcgaag acctcgggat
     3601 tctcgacgcg cacgccgacg aggtccgtga cgtcgaagaa tcgccggtag ttgatcttct
     3661 cgcttgcgat gcgccagtag gcgaggcggt acgactgcgc gtccagcagc gcatcgagcg
     3721 cgttgaagct gtccggtttt ccctgcttgc cgttgatccg cgcgatcgcc gaatgaaggg
     3781 cgtcgcgaaa cgcttcgtcc tgctcgtgga tgcgccagag cgcatccttc aggaaacggc
     3841 tgttcggcac ggaatcatgg ttctcggtca gctcgcgcaa ctcaatcgaa acgctgttct
     3901 ctgggaggaa ttcgaggcag gcgcggagca cgagactgta ggagtctggc gaaaggggga
     3961 ggcgtttgtc gtagtaggtg aaaaagaagc cgtcggcgtc gaagtgaagc tggatctcct
     4021 gagcctcgag cacctcgccg tacggtttgc cgaggatggg gaggagaacc tttgccgcgg
     4081 tgtgcccgcg cgtcgtaacc ggggtgaggt cgatgtcgaa gtagtggagg aagcgcgagt
     4141 gctcaccgtt ctcgagaacg ctcatccacc atgcgttttc ggcgctggcg gccatgtggt
     4201 tgggaacgat gtcgagcagc aggcctagct tccggcgctc aagctcctgg tgtagcgcgt
     4261 cgaaatcgtc gtcgcttcca agctccggat tcagcgagtt ggcgtcgacg acatcgtatc
     4321 cgtggctgct gccttttcgg gcgcgcagga tcggtgacgc gtagacgtcg ctgatgccga
     4381 gctcttcgag atagagcgcg atcctctggg catcggcgaa ggtgaagccg gcgttgaact
     4441 ggatgcgata ggtggcccga ggtacgcggt acatgtgcgg ttgagtgcgc tgcgaaaggg
     4501 gtgccgtgtt ggcggtttac gtcctgtgac gtgtcgtggt gggtgccgga ggcgctggcg
     4561 ccgcaatatg cgcgcgcacc tgcccaccga ggtgacggat tgcggcctac ttggccgcac
     4621 gtggggtgca agttgaccgc attgcggcgc ttcctgtccg catgatgcgg cgcaggttga
     4681 ccgcattgcg gcgcttcctg tccgcacgtg gggcgcaagt tgaccgcatt gcggcgcctc
     4741 ctgtccgcac gcgcggcgcg agttgaccgc attgcggcgc ttcctgtccg catgtggggc
     4801 gcaagttgac cgcattgcgg cgcttcctgt ccgcacatgg ggcgcaagtt gaccgcattg
     4861 cggcgcttcc tgtccgcacg tgcggcgcga gttgaccgca ttgcggcgct tcctgtctgc
     4921 acgtgcggcg cgtgttgatc gcattgcggc gcttcctgtc cgcatgtggg gcccaagttg
     4981 accgcattgc ggctcttcat gaccgcacat gcggcctgag gtgaccgcgt tgccgtacgc
     5041 cgtgtccgca ctgaggcccg attgggcatc gttgcggcac gtcgtggcca ctgggcaccg
     5101 gaggcgcaac gttgttgcgc gccgggtttc gcaagtgcgc gccgagggtg tagcgctgtc
     5161 gacaaaggat gcactcgacg tgttgccgcg tccaaggttc gacggtctcg acgccgtccg
     5221 tcacgaggat gacgatgtcc tgccggtggc cactctacac ccgtcgcatt acgacgatcg
     5281 tcttgtcgtc tgtggctttt gcgccgcgct cgaacgcgat gacggcgtcg agaacacgtg
     5341 aggggatgtc gttcgttggt tcgttgcgtt gggcgaggaa gagttcgcgc agattgtcgt
     5401 tgccgaattc gacgtcgtcg gggtcggcgg cttcgttgag gccatcggtg taaaggaaga
     5461 gtacggagcc gtgcggcaac tcgatcgtgc cttccgtgta ggacgactcg gggaggattc
     5521 cgatcggtct gccggtcgat ccgatctctg tcagtccctc ggccgtggcg atatatggcg
     5581 gattgtggcc ggcgttgacg tagcgcagca cgcctgtggc gggcgagtaa cgggcgaaga
     5641 agaacgtcgc gtatttgcgg ccgcgggcgc tgcggaagat cagcgcgttg aggcgtgcga
     5701 cgagacgggc gagatcgtcc tcgatcggcg cggcaacgcg tagcgcggcc tgcacgctcg
     5761 acatgatcag cgcggcgccg acgcccttgc cggagacatc gccgatggcc agaccgaggt
     5821 cgccgccagg gagctcgagg aaatcgaaat agtcgccgcc cacttcgtag cagggaaggc
     5881 ttagcgcacc gagctcgatg cctttgacgt cgactggcgc tgacggcaga agacggctct
     5941 ggatctctcg gccgagggcc agttcctgtt ccatgcgctg cttctcgagc agctccatgt
     6001 gcaacgtggc gttctccatg gcgatggcca tgtggtcgga gatgtcgttc aggaactcca
     6061 gatcgcgcgg tccgaacgag cctttgatct tgttcagaag ctgcagcacc ccgaccacgc
     6121 gctcgccgcg gttcttgatc ggaacgcaaa gcatcgagcg cgtccggtag ccggttttct
     6181 gatcgagcga gcggtcgaac cgcgggtctt gataggcgtc gtggaggatg acagcttcgc
     6241 cggtctccgc aacctttccg gctagcccct tgccgatcgg cagacggatc tcggtggcca
     6301 gctcgccggc gatcttggtc cagatctcct tcttctgatc gtcgacgaag tagagcgtcc
     6361 cgcgctccac gtgcagctcg ttgcgggcga cggtgagaat ggaggcgaag agtgcgttgg
     6421 gctcgatcga ggagttgatc agcttcgatg cttcgacgag cgtgcgcagg cgctccatct
     6481 catcgcggtc ggcgagcgtg gtgacgatgc gcccgacgag gatgcgatcg cgctcgctga
     6541 actgcaggcc gctctgaaac accgtctccg agcgctcgct gcgcacgatc gtgcaatcga
     6601 cctcgatctg ctcgccgtcc caatcgaatt gcaaagtgtg cggaccggcc atgagcggct
     6661 cctcgtgctc gatgcgcgtg cctaggatgc cgatgtcgac gatgcggacg acgctttcgg
     6721 cgactcgggc tggaatcggt gcaggaagga gtaatcgctc ggcacgacgg cggtcttcat
     6781 cgagcatcgc gagattgtat gccgttcgcg agttaggttg gttgcgcttc gcgcgttgtc
     6841 ggttgtcggt tatcttcgcg ttgcgccttg tcgtccaagt agcgcttcgg agtgggtagc
     6901 ctcccggctt actaaacgcg cgaagcgcaa ccaaccgaca accgacaacg cgcgaagcgc
     6961 aatgttcgag gcttttcaag aatccgacta ccgccgcttc tgggtggcgc agttcatctc
     7021 caacatcggc acctggatgc aggggctggc gcagggatgg ctcgtctaca agctcacgga
     7081 gtctccgttc ctcctcggct tcgtggcatt tgccaactcg gcgccgtcgc tgattctcat
     7141 gctgccggca ggcgttctcg ccgatcacct cgaccggcgc cgcgtggtcg gcctctcgca
     7201 aatcgcgcag gcgctggcgg cgttgttcgt ggccatctcc atccgcaccg gccacatcac
     7261 cgtgtggcac atcgtcgccg cgtcggcggt cagcggcgtg gcca
//
LOCUS       DATLVW010000765         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_610290, whole genome shotgun sequence.
ACCESSION   DATLVW010000765 DATLVW010000000
VERSION     DATLVW010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610290"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VNN25_22410"
     CDS             <1..949
                     /locus_tag="VNN25_22410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH94347.1"
                     /translation="DWLNEIANSLLIGGATVLALDLGRRLFRRSQIFCPEPDPVISWN
                     PEDYGIPHGQTEVLRFETDDGEELYGWYCRAKNPIASALYCHGNTGNLSTPAHVMPYL
                     LDAGINILLFDYRGFGLSTGTPSFSGVIDDGVTAARLHEKIRPKNVPSILYGFSLGGA
                     VAAQIIRRYPFDGMILQSTFTNLPDLARVAFPRVPLHLVSGRLFDTLSVVRNLKVPLL
                     LIQGSADDVCPAWMAQQLHDNCPSASKKLMLVDGGQHKDLFAREDAQEIVWMINRFAT
                     DLPRTPHVVQDEPRPAEMLLDRALRYVRRHRRRLVQHPL"
     gene            965..2086
                     /locus_tag="VNN25_22415"
     CDS             965..2086
                     /locus_tag="VNN25_22415"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXH94348.1"
                     /translation="MLAVVKSAAVDGPEGTSVRDCPVPKPGPDEVLIRVAAAAVCGTD
                     KHIYHWDPSIRDKVTPPRIYGHEFCGFVEEIGARAQRGYEGHLEAGDYVSAEMHVVCG
                     ECPRCRTGNGHICDRTRIYGLHEDGAFAEFVKVPSSNVVKLAPYVPLRVGAFLDALGN
                     AVHATQICDLAGKSVAITGFGPIGAMAAAIAEHSGAATVVVTDVSDHALETARRWAAI
                     HGFKNLHAFNVRSMAADEVKAEVDAVTDGDGVDLVLEMSGAPAAINLALDIVRMGGTL
                     SLLGLPAGHAVTINDYTRNVIFKGVTMQGIIGRRMYGTWQRMLALLKSGLDVEWIVQA
                     AFDSLNDFHEGVARFDRHEALKVVFFPEGESAANARLRK"
     gene            2130..>2605
                     /locus_tag="VNN25_22420"
     CDS             2130..>2605
                     /locus_tag="VNN25_22420"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXH94349.1"
                     /translation="MKRIAILTGGGDVPGLNAAIKSFVWRLLENEDCEIFGLRRGWAS
                     LINIIPEAGADNSAWITPLNRMNTRAIDRFGGTMLHTSRMNPQISKQEHIPEYLEASK
                     GAPDAGGRYDLTSAALRTINFLQLDGLVALGGDGTLTFARRLHNEGVPLVAIPKTM"
BASE COUNT          453 a          866 c          799 g          487 t
ORIGIN      
        1 cgactggctt aacgagatcg caaactcgtt gttgatcggc ggtgcgacgg tcctggccct
       61 cgacctcgga cgccgcctct tccgacgttc ccagattttc tgccccgagc ccgaccccgt
      121 catcagttgg aatccggagg attacgggat cccgcatgga caaaccgagg tcctgcgctt
      181 cgaaacggac gacggggagg agttgtacgg ctggtattgc cgagcgaaga atccgatcgc
      241 ctccgcactc tactgccacg gaaacaccgg caacctgtcc accccagcgc acgtcatgcc
      301 ctatttgctg gacgccggaa tcaacatcct tctttttgat taccgcggct tcgggctgag
      361 caccgggacg ccttccttct ccggcgtcat cgacgatggc gtcaccgccg cgcggctgca
      421 cgagaagatc cgccccaaga acgtcccctc gatcctttat ggcttctcgt tgggcggcgc
      481 ggtcgctgca caaatcatcc gccggtatcc tttcgacggg atgatccttc agtcgacatt
      541 cacgaacctg ccggacctcg cacgggtcgc gtttccgcgc gtgccgctgc atctcgtcag
      601 cgggcgcctt ttcgatacgc tcagcgtcgt ccgcaatctc aaagtgccac tgctgctcat
      661 ccagggctcc gcggatgacg tctgtcccgc gtggatggcg cagcagcttc atgacaactg
      721 tccgtcagct tccaagaaac tgatgctcgt cgatggcgga cagcacaagg atctcttcgc
      781 ccgcgaggac gcgcaggaga tcgtctggat gattaatcgc ttcgccaccg atctgccgcg
      841 cacaccgcac gtcgtgcagg atgagcctcg cccggcggag atgctcctcg accgcgcgct
      901 gcgttacgtc cgccggcatc gccgccgcct ggtccagcat ccgctgtgat agcgtccccg
      961 caccatgctt gctgtcgtca aatcggctgc tgttgatggc cccgagggca cgtccgtccg
     1021 tgattgtccc gtcccgaaac cgggaccgga cgaggtcctg atccgcgtcg ctgcggctgc
     1081 cgtctgcggt accgataaac acatctacca ctgggatccg tcgatccgcg acaaagtcac
     1141 gccgccgcga atctacggcc acgaattctg cggcttcgtc gaagagatcg gcgcgcgggc
     1201 gcagcgcggt tacgaaggtc acctcgaggc cggcgactat gtttccgccg agatgcacgt
     1261 ggtttgcggc gaatgtccgc gatgccgcac gggcaacggc catatctgcg accgcacgcg
     1321 tatttacggt ctgcacgagg atggcgcgtt tgcggagttc gtgaaagttc cttcctcgaa
     1381 tgtcgtgaag ctcgcgccgt acgtcccgct gcgtgtcggc gcgtttctcg acgcgctcgg
     1441 caacgcggtt cacgccacgc agatctgcga cctcgccggc aagtcggtgg ccatcaccgg
     1501 atttggcccg atcggcgcca tggccgcggc catcgcagag cacagcggtg ccgccacggt
     1561 ggtcgtgacc gacgtcagcg atcacgcgct ggagacggcg cgccgctggg cggccatcca
     1621 cggtttcaag aatctccacg cgttcaacgt ccgctcgatg gcggccgatg aagtgaaagc
     1681 ggaagtcgat gccgtgaccg acggcgacgg cgtcgatctc gtgctcgaga tgtcgggcgc
     1741 gcccgctgcg atcaatctcg cactcgacat cgtccgcatg ggcggcacgc tttccctcct
     1801 cggccttccc gcgggacacg cggtcacgat caacgactac acgcgcaacg tgatcttcaa
     1861 aggcgtgacg atgcagggca tcatcgggcg gcgtatgtac ggcacgtggc agcgcatgct
     1921 ggcgctgctg aagtcgggcc ttgacgtcga gtggatcgtg caggccgcgt tcgattcgct
     1981 gaacgatttt cacgaaggcg ttgcacgttt cgaccgccac gaagcgctga aagtggtctt
     2041 cttccccgag ggagagagcg cggcgaatgc gcggctgagg aagtgagcgt cgcggcatgc
     2101 gcgggcggct cgcgttgttt cgctgactga tgaaacgaat tgccattctt accggcggcg
     2161 gcgacgtccc gggactcaac gctgcgatca agtcgttcgt gtggcggctg ctcgaaaacg
     2221 aagattgcga gatcttcggc ctgcgccgcg gctgggcttc gctgatcaac atcatccccg
     2281 aggcgggcgc ggacaactcg gcgtggatca cgccgctcaa ccggatgaac acgcgcgcca
     2341 tcgaccgctt cggcggcacg atgctgcaca cctcgcgcat gaacccgcag atcagcaagc
     2401 aggagcacat tcccgagtat ctggaagcgt cgaagggtgc gcccgacgca ggcggacgct
     2461 acgaccttac gagcgcggct ctccgcacca tcaacttcct ccaactcgat ggtctcgtgg
     2521 ccctcggcgg cgacggcacg ctcacgttcg cccgccgtct tcacaacgag ggcgtgccgc
     2581 tcgtggccat ccccaagacg atgga
//
LOCUS       DATLVW010000766         5662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_610312, whole genome shotgun sequence.
ACCESSION   DATLVW010000766 DATLVW010000000
VERSION     DATLVW010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610312"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VNN25_22425"
     CDS             complement(<1..981)
                     /locus_tag="VNN25_22425"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH94350.1"
                     /translation="MTDPSLRGNLTSFRLPDVLTFLASARKSGTLTLTSTGKEATVFF
                     DNGSVVYAGSNQEPLRLGAVLLRKKKISAEQRAAIDKLMHAEGGRFGQIAVQQGLLTE
                     DQLRDFLKVQASEIIYDSFVWPGGTFAFIDDTTLPAYAVTIAIDLSNLIMEGARRIEE
                     WEECERLLPDASVVFKVVSNPDAEKITLSLDEWKILFLINGRRKLGELALAADEEPLH
                     VYRVIYGLFGNKLIEPVTHAPSPDDTSRTKQTMPPLEDTMRQKPATFSADSTVREVAD
                     DTNLLVSTEAKLSYKDVVKPTIAQLTITNGEDAGLMFPLTESEYLIGRQRD"
     gene            1028..2233
                     /locus_tag="VNN25_22430"
     CDS             1028..2233
                     /locus_tag="VNN25_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH94351.1"
                     /translation="MQSNSRHAVVAGFLGWTLDAFDFFVVVFLLDTLALQFGVPKSRI
                     ILTLTATLALRPVGAIIFGMLADRYGRRTPLMANVIYFSVIELLSGFSMTFTQFLVLR
                     ALFGIGMGGEWGVGASLALENAPTKWRGVLSGILQSGYSIGYLLAAIATRLVLPHYGW
                     RWMFWIGAIPALLALYIRTKVPESQAWKEHRVGSFGEMLRAIQGQGRSFLYLVLLMTF
                     MMFLSHGTQDLYPDFLKNAHGVPATTVPYVAMLYNVGAVVGAIIFGQLSQKIGRRYSM
                     IAALIVSLLVIPMWAFGGSLVILAAGAFLMQVGVQGAWGIIPIHLNELAPDAVRGLMP
                     GLAYQFGILVAAPTNSIEDALRARFGYSWALAGFEVFVMVVLTVILLFGRENHGRSFH
                     SGDLVIETD"
     gene            complement(2326..4194)
                     /locus_tag="VNN25_22435"
     CDS             complement(2326..4194)
                     /locus_tag="VNN25_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94352.1"
                     /translation="MSVPKGLIVTLLVLFALPTFAADNRWSIQGPDGSSVNRLVFDPA
                     SSSIAYAGTTNGIFRSADGGQHWTGASELLGTPINDVAVAAGDPQKVFVATSYGLYKS
                     NDRGLTWAVVHGFGSYAVAVSRTNANIVYSDSTGGPFRSSDGGATFGSVGSGLPSAAA
                     AVSVLAVDPQSPDTVYAAYQTNVGVYKSVDGGAHWTPANTGLPQAIVFSMVIDPSNGS
                     TLYAGSAPGLFKSTDGGGSWTALTNGLPSSPFCYSLSISAGTIVAGIGGGLYKSMDGG
                     ATWTSAGFNGNTVNPAAIDPANPANILASANSVTFKSTNGGANFAVTSGLTAFYTLAI
                     AVDPQNASVVYAGGSTGVFKSVDRGVSWTSLLTNQTGSIAVDSQNSQTVYANPFGTVR
                     RSTDGGVTWSDFTAGLPAGSALVVAADPQVSGTIYTILGNAVYKKIGSASWVKASTGI
                     PNGSLTFITIDRNNSSTLYAGGGIGIYKSIDGGTTWTATGRPAGGVTPTTVIIDPFDS
                     GHLFSWSGSSAFESTDGGANWSSIVVAGGRQAITLAFDPSAPGRIYSSGADNNGVGTI
                     DRSNDGGKTWFSLRTGLGRGLNTIFVVAPGGNMLYAGSTNGGVWAFHFARARAAGK"
     gene            complement(4298..4711)
                     /locus_tag="VNN25_22440"
     CDS             complement(4298..4711)
                     /locus_tag="VNN25_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94353.1"
                     /translation="MSATVLYRIAAIVLILFAAGHTAGFLTFKPPSAEGTAVRDAMNG
                     VHFAFGGKTYTYGGFYKGFGLTITVYLLFSAVLAWHLGGLAAAHPEAIGILGWAFFAL
                     QIASLVLTWMYFFPVPAIFAGVVALCTGWAAVVAR"
     gene            complement(4867..5595)
                     /locus_tag="VNN25_22445"
     CDS             complement(4867..5595)
                     /locus_tag="VNN25_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94354.1"
                     /translation="MKRLIGMICTLAALRAGADTLGDVRAAVGRLSAKPPVRATFQSK
                     SFSAASGRFGNDKVQRNVSAEVAHEAAGVSITVPQTLLDDVSRARNRGDASAQNLIGS
                     IGTVAITDALDFRDSFLAMLDHAVVTREERIPFQGKPTRHLLLKLPPRPKQTGTIDIG
                     TVKTDDELSLWVGDDSLPLAAERTQKTTAGFMFFHGTYAGRTSYTFAHTANRLMVARL
                     ETSGNGAGLGQNIETTAVQTLTLH"
BASE COUNT         1053 a         1648 c         1827 g         1134 t
ORIGIN      
        1 gtcgcgttga cgtccgatca gatactccga ctcggtcagc ggaaacatga ggcccgcatc
       61 ttcgccattc gtgatcgtga gctgggcgat cgtcggcttg accacgtctt tgtacgagag
      121 cttcgcctcc gtcgagacga gcaggttcgt atcgtcggca acttctcgca ccgtcgagtc
      181 agcgctgaac gttgccggct tctggcgcat cgtgtcttcc agcggtggca tggtctgctt
      241 cgtgcggctg gtatcgtcgg gtgatggtgc atgcgtaacc ggctcgatca gcttgttgcc
      301 gaagagtccg tagatgacgc gataaacatg aagcggctct tcgtcggccg ccagcgccag
      361 ctcgccgagc ttgcgccggc cgttgatgag gaaaaggatc ttccactcat cgagcgagag
      421 cgtgatcttc tccgcgtcgg gattcgaaac gactttgaag accacggaag cgtcaggaag
      481 aagacgctcg cattcctccc actcttcgat ccgccgcgcg ccttccatga tgaggttcga
      541 cagatcgatg gcgatcgtca cggcgtacgc agggagggtt gtgtcgtcga tgaaggcgaa
      601 cgttccgccg ggccagacga aggagtcata gatgatctcg gaggcctgca ccttgaggaa
      661 gtcgcggagc tgatcctcgg tgagcagccc ctgctgcacc gcaatctgcc cgaaccttcc
      721 cccctcggcg tgcatcaact tgtcgatggc agcgcgttgc tcggcggaga tcttcttctt
      781 gcgcaggaga acggcgccaa gccggagcgg ctcctgattc gatcccgcgt agacgacgga
      841 gccgttgtcg aagaagaccg tcgcttcctt gccggtgctc gtgagcgtga gcgtgccgct
      901 cttgcgcgct gacgccagaa acgtcaacac gtcgggcagc cggaaggaag tgagattccc
      961 gcgaagcgac ggatcagtca ttcggtgcgg agattgtagc aagcacctca attacaattt
     1021 cgaaccaatg caatccaact cacgccacgc cgtcgtcgcc gggttcctcg gctggacgct
     1081 cgatgccttc gatttcttcg tcgtcgtctt ccttctcgac acgctggcgc tgcagtttgg
     1141 agtgccgaag tcgaggatca tcctcacgct caccgcgact ctggccctgc gccccgtcgg
     1201 cgcgatcatc ttcggcatgt tggccgatcg ctacgggcgg cgcacgccgc tgatggcgaa
     1261 cgtcatctac ttctcggtga tcgagctgct ctccggcttc tcgatgacgt tcacgcagtt
     1321 tctcgtgctg cgcgcgcttt tcgggatcgg catgggaggg gagtggggcg tgggcgcgtc
     1381 gctggcgctg gagaatgcgc caacgaagtg gcggggcgtg ctgtccggca tcctgcaaag
     1441 cggctactcg atcggctatc tgctcgcagc gattgccacg cggctggttc tgccgcacta
     1501 cggctggcgc tggatgttct ggatcggggc gataccggcg ctgcttgcgc tctacatccg
     1561 gacgaaggtc ccagagtcgc aggcgtggaa ggagcatcgc gtgggcagct ttggcgagat
     1621 gctgcgcgcg atccagggac agggccgcag cttcctttac ctcgtcctgc tgatgacgtt
     1681 catgatgttt ctgtcgcacg ggacgcagga tctctatccc gattttctca agaacgcgca
     1741 cggcgttccg gcgacgacgg tgccgtacgt ggcgatgctc tacaacgtcg gcgcggtggt
     1801 cggcgcgatc atcttcgggc agctttcgca gaagatcggc cgccgctaca gcatgatcgc
     1861 cgcgctgatc gtgtcgctgc tggtgattcc gatgtgggcg ttcggcggat cgctggtgat
     1921 cctcgccgcc ggagcgttcc tgatgcaggt gggcgtgcag ggagcgtggg gcattatccc
     1981 catccacctc aacgagctcg cacccgatgc cgtgcgcggg ctcatgcccg gactggcgta
     2041 tcagttcggg attcttgtcg ccgcaccgac gaactcgatc gaagacgcgc tacgggcccg
     2101 gttcggttac tcctgggcgc tcgcggggtt tgaggtcttc gtgatggtgg tgctgacggt
     2161 gatcctcctc ttcggccgcg agaatcacgg aagatcgttc cattcggggg atctggtgat
     2221 tgagacggat tgatgattcc cggcaaaccg taggcaccac agagacacag aggccacaga
     2281 gaaatgcttt atctctgtgc tctctgtgtc tctgtggtgc attcttcact tacccgccgc
     2341 gcgcgctctt gcgaagtgaa atgcccaaac gccgccgttt gtgctgcccg cgtagagcat
     2401 gttgccgccc ggcgcgacga cgaagatggt attgagtccg cgcccgaggc cggtgcgcaa
     2461 tgagaaccag gtcttgccgc cgtcgttgct tcgatcgatt gtgccgacgc cgttgttgtc
     2521 ggcgccgctg ctgtaaatgc ggccgggagc ggaggggtcg aatgcgagag tgatcgcctg
     2581 ccgcccgccg gccacgacaa tggagctcca attggcgccg ccgtcggtcg attcgaacgc
     2641 ggacgagccc gaccaggaga agaggtggcc ggagtcgaac ggatcgatga tgactgtggt
     2701 gggcgtaaca ccaccggcgg gccgtcccgt cgcggtccag gtggtgccgc cgtcgatgct
     2761 cttgtagatt ccgattcctc cgccggcata gagtgtggat gagttgttcc ggtcgatcgt
     2821 gatgaatgtc agagaaccgt tcgggatgcc cgtgctcgct tttacccacg atgcgctgcc
     2881 gatcttcttg tagaccgcgt tcccgaggat tgtgtaaatc gttccgctaa cctgcggatc
     2941 ggcggccacg actaacgcgg agccggctgg gagtccggcc gtgaaatcgg accaggttac
     3001 gccgccgtcg gtgcttcggc ggacggtgcc gaatgggttg gcgtagacag tctgcgaatt
     3061 ctgggaatcg acggcaatgg atcctgtctg gttcgtcagc agcgacgtcc agctcactcc
     3121 gcggtccaca ctcttgaaca cacctgtgga acctcctgca tagaccaccg aagcgttttg
     3181 tggatcaacg gcgatcgcga gcgtatagaa cgcggtcaag ccagacgtga ccgcgaaatt
     3241 cgctccgccg ttcgtgctct tgaaggtgac cgagttcgcg ctggcgagaa tatttgcggg
     3301 atttgcagga tcgatggcgg caggattaac tgtgttgccg ttgaagccag cagaagtcca
     3361 ggtcgctccg ccatccatcg acttgtaaag gccgccgccg attcctgcga cgatcgttcc
     3421 tgcgctgata gagagcgaat agcaaaaggg cgaagagggg agcccgttcg tcagcgcggt
     3481 ccacgatccg ccgccgtccg tcgatttgaa caacccgggc gctgagccgg cataaagcgt
     3541 ggagccattc gacggatcga tgaccatgga aaagacgatt gcctgaggca ggccggtgtt
     3601 tgcgggtgtc caatgcgctc cgccatcgac tgatttgtaa acgccgacat tggtctggta
     3661 tgcggcgtag accgtgtcgg gcgattgcgg atcgacggcg agaaccgaga ccgcggccgc
     3721 tgcggagggt agaccgctgc cgacgctccc gaacgttgca ccgccatccg aggagcggaa
     3781 gggtccgccc gttgaatcgc tgtagacgat gttggcgttc gtgcggctta cggccacggc
     3841 gtaggagccg aacccatgaa cgaccgccca ggtcaggccg cggtcattgc tcttgtagag
     3901 tccatacgac gtggccacga acactttttg ggggtcgccg gccgcgaccg caacgtcgtt
     3961 gatcggtgtt ccgagaagct ccgaggcgcc tgtccaatgc tggccgccat cggcgctccg
     4021 gaagatgcca ttcgtggttc cagcataggc gatggacgaa cttgcaggat cgaacacgag
     4081 ccggttgacg ctgctgccat ccggcccctg gatgctccag cggttgtcag cggcgaaggt
     4141 gggaagagcg aagagaacga gtagcgtgac gatgaggcct ttggggacgg acatgccggc
     4201 attttacggc tgcaaccacg tctttttgga aatatccggt accagatccc gcagtcaact
     4261 tatgtaagtc atacgacgtc gactctctga ccacgcgcta ccgcgccacc accgcagccc
     4321 accccgtaca gagcgcgacg acaccggcga agattgcggg cacaggaaag aaatacatcc
     4381 acgtcaaaac gagcgatgcg atctgaaggg caaagaatgc ccatccgaga ataccgatcg
     4441 cctcgggatg cgcagcagcg agaccgccga ggtgccacgc gagcacggcg gagaagagaa
     4501 gatagacggt gattgtgagc ccgaagcctt tatagaaacc gccgtaggtg taggtcttgc
     4561 cgccgaacgc gaagtgaacg ccgttcatgg cgtcgcgaac cgcggttcct tctgccgagg
     4621 gtggcttgaa tgtgaggaat cccgcggtgt ggccagcggc gaacaggatc agaacaatcg
     4681 cggcgatgcg gtaaaggacg gtagcgctca tcaagtctcc ctgcgtccat gtgacgatcg
     4741 atgaagtctg gcggcggcag agtttactgc tgagctggcc aaagcgaaaa gtcccgtgcg
     4801 aggcaagctt tagctcgcaa ctctcacccc catatccttg gctccttcca atcgagcgcg
     4861 atcaagtcag tggagcgtga gcgtctgcac tgcagtggtc tcgatgttct gacccagacc
     4921 tgcgccgttc ccggatgtct cgaggcgcgc aaccatgaga cggttggcgg tgtgcgcgaa
     4981 ggtatagctc gttcgtccgg catacgtgcc atgaaagaac atgaagccgg cggtggtctt
     5041 ctgggtgcgc tccgcggcga gagggagcga atcgtcgccg acccatagcg ataattcatc
     5101 atcagtcttc acggttccga tatcgatcgt gccggtttgc ttgggacgcg gcggcaattt
     5161 gagaaggagg tgacgggtcg gtttcccctg gaacgggatg cgttcttctc gcgtcacgac
     5221 cgcatggtcg agcatcgcca gaaacgagtc gcggaagtcg agcgcatcag tgatcgcgac
     5281 ggtcccgatc gagccgatca ggttctgcgc ggatgcgtca ccgcgattgc gcgcgcggga
     5341 aacgtcgtcg agcaacgtct gcggaacggt gatcgagaca ccggccgcct cgtgagcaac
     5401 ttccgccgaa acgtttcgtt gcaccttgtc gttgccgaaa cgtcccgatg ctgcggagaa
     5461 cgatttggac tggaaagtag cccgaaccgg aggcttcgca gagagacggc cgacggcagc
     5521 cctcacatca ccgagcgtat ccgcgccggc gcgcagcgcc gcgagtgtgc aaatcatccc
     5581 gatcagtcgt ttcatggatc tctccttgga atgtagacgc gccgcgaact ggaaggttac
     5641 tgatggctgg ggagtggggt gt
//
LOCUS       DATLVW010000767         8076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_402395, whole genome shotgun sequence.
ACCESSION   DATLVW010000767 DATLVW010000000
VERSION     DATLVW010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402395"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            650..1318
                     /locus_tag="VNN25_22450"
     CDS             650..1318
                     /locus_tag="VNN25_22450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94355.1"
                     /translation="MSASNPLPVTTSPAVTAATTADAIAEQFRAVLVTLLAIVPDLQK
                     YDSRRIRRIAASAKFGDVAIVPVINLVASVPRIKESNLFDVDAGHFARQFKDQVVPLA
                     MQLIEFANDVIFTSDTLLAASSVQGLQVYRLAQHAVKQPDGADLQPFVDEMSHIVKKT
                     MNYHRKPVPAPTPSAPTTSHTPSTPGAQGFLASNLVPDKTSEPEEDVADHFEQALKDV
                     TKKP"
     gene            complement(1376..2449)
                     /gene="arsB"
                     /locus_tag="VNN25_22455"
     CDS             complement(1376..2449)
                     /gene="arsB"
                     /locus_tag="VNN25_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HXH94356.1"
                     /translation="MDAAAPKRMNIFERYLSLWVALCMVAGVILGKLLPGSVRALQRM
                     EFGTGSQINIPIAILIWLMIYPMMLKIDFGSIVRVGRKPKGLLITVVVNWLVKPFSMA
                     FFGWLFFRHLFLPLIGPALASQYIAGVIILAAAPCTAMVFVWSYLSDGDPAYTLVQVS
                     VNDLIMLVAFAPIVKFLISGAAGLEVPFRVLLYSVLIFVVIPLSLGAISRAVLIRRKG
                     AEWFERELMTRFAPVTIIALLATLVLIFAFQAENITTRWFHVLLIAVPILLQVYFNSS
                     LTYGLMKLFRVPYPIAAPGALIGASNFFELAVATAIALYGPGSGAALATVVGVLVEVP
                     VMLSVCRMCVSTKHWFEQPARTS"
     gene            2616..3902
                     /locus_tag="VNN25_22460"
     CDS             2616..3902
                     /locus_tag="VNN25_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94357.1"
                     /translation="MNGLILNWFLSVLVVALPGVAAEKDDAHNMPGMQMQSDATSELL
                     MRQASGTGMNPNAAPMHMAMTQYGDWMLMLHGLAFVNHVVQSGPRGDDKLFSTNWFMG
                     MADRPLGGGHLMLRSMLSLEPLTVGKKYPELFQTGETIGGRPIIDAQHPHDFFMELAA
                     EYAHPLTGNTIGYIYAAPFGDPSLGPVAYPHRASASEIPQAALSHHVQDSTHIAGSVL
                     TIGAQSGMFGYAFSGFHGREPDEKRWDIDTGKIDSWAARFTFDPSPNWTAQISTGHLK
                     HPEAAEPGDVQRTTASIAYSDVTSIGQWDTSLVFGHNNKSEGHDGSSWLAESVLQFGG
                     RNYVAARAEAVDKDELFAEQNVPPAIEHGLFRVKALTLGYSRDLLTTSEITGALGGNV
                     TFYSLPDAIKPFYGSSPHSVYFFIRLRGGSAGAHSM"
     gene            complement(3981..4952)
                     /locus_tag="VNN25_22465"
     CDS             complement(3981..4952)
                     /locus_tag="VNN25_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXH94358.1"
                     /translation="MHAMVLREAGKPLELIELPVPQPNAEQLLLRVECCAVCRTDLHV
                     VDGELRDPKLPLVPGHQIVGIEEGTNTRFGVPWLAWTCGVCVYCRSERENLCDRAKFT
                     GYTVDGGYAQYAVADRRYCFRIPESYTPAEAAPLLCAGLIGFRAYRMAGDAARIGFFG
                     FGSAAQILVQIARADGREVYAFTRDEDSAAQQFARDMGAVWASGSSVTPPHPLDAAII
                     FAPEGSLVVTALRNLVKGGTVICAGIHMSDIPSFPYKMLWNERSIRSVANLTRADGDQ
                     FFQRIASLRIETKRTVYPLDRANEALADLRAGRLTGTAILDPWTSRD"
     gene            complement(5610..6413)
                     /locus_tag="VNN25_22470"
     CDS             complement(5610..6413)
                     /locus_tag="VNN25_22470"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXH94359.1"
                     /translation="MLLVLLAACTANEPAPKQTPRRIVTLAPNVTEMVLALGAGDRIT
                     ATDDFSADAIADATPGRKLPHVGGIQPNLEKIVAVRPDLVIANAAGLPPNLGRALAAV
                     HIPLLVVTNERLADIARSMAAIGTALGVDSTPAVAALNASLARARRTRAQPPRVMLAV
                     WPDPLYVAGNNTFAGDLFALCGARNAVEVNGWPQYSLESFVASPPDLLLYPNHSVAPE
                     QVRALLDRAGTKNVEAVAVDENLFTHPGPKVGKAAEMLNAILDARVKKP"
     gene            complement(6516..6746)
                     /locus_tag="VNN25_22475"
     CDS             complement(6516..6746)
                     /locus_tag="VNN25_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94360.1"
                     /translation="MTVITDQQRKFYETTLQVTKQEISDLKDQIEEELAKVKDRIAEL
                     QNAINASKQMYAAACSRLGINNDMDDDDGGES"
     gene            complement(6780..>8076)
                     /locus_tag="VNN25_22480"
     CDS             complement(6780..>8076)
                     /locus_tag="VNN25_22480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH94361.1"
                     /translation="YMSPEQAWGRNIDRRSDIFALATVLFEMLTARKLFSGDNELSIL
                     EQVREARVTKPSELNDEVTPQIDAIVLKALQKDPANRYQTAGEMQRDLDAVLYNFRPT
                     PTSADLAIYMHRLASSAVVEQHHVEPIPMPEPAPAANELRPIKPAAVAVPLPMPAAIP
                     AAIPAAAESAKKPPIVPIAIAAAVVIAVIVGFLMMRKPATTSVAAPGKVPVAGKPLIA
                     TTTSTSTSAPVTGSTSTVATAPVIDASKVNEEVQKRMAAEKTRLEQLARTQSTPATTT
                     NRGTAPPTPAPQPQVAQAVPPPVVENRPVPAPQPVVPVPQPVPESRPAVQPESPRTQL
                     GDLVSQGTEGLNPARVARQAAAQYPPIARVQRMQGSVVVSALVNENGQVIDTRVVNSA
                     SPILNSAAIESIKRSTFTPGTKDGVRVKSWTTVRIDFKL"
BASE COUNT         1512 a         2422 c         2612 g         1530 t
ORIGIN      
        1 cgtcatcggt ccgttctgtt ccgtgatcgg tccgtttcgt tccgtgatcg gtccgtttcg
       61 ttccgtgatc ggtccgtttc gttccgtgat cggtccgttt cgttccgtga tcggtccgtt
      121 tgattccgac ttattaccgc cccttcccgt cttttggccc gcagttctcg cacttgcact
      181 tggaattgcc ggcagtcgct cccaatcgag cgccttctcc cccacgcttc tcagtggggg
      241 agaaggtggc cgaaggccgg atgagggggc gtttgtctgt cgcgccaacg ttcgcaaaca
      301 ccctgcaagg acgccctttc aatcaggtaa cgctcccaaa gaacgtgttt gtcccatctt
      361 tcgacgcccc cctcatccgg cttcgccacc ttctcccccc gcaaaaagcg cggggggaga
      421 aggcgctcga tgggaggagt cgcagaaatg tgtgagaaat gcgggttagc cttctctttc
      481 cctcggttga ggaacctttg tccgggtgac ccgtctcaag tatgtctgaa ccgtttcaga
      541 ccgcgccgtc agcgttttcg cgggcgacgt gattggcagg cccgtggcgg ccacacgatg
      601 gataacacgc gttgggagat cgagcgcgtg ccttaaccgg agattgaaga tgtcagcgtc
      661 taaccctcta cctgtcacca ccagtccagc agtaaccgcg gcaacgacgg ccgatgcgat
      721 tgccgagcag tttcgcgccg tccttgtcac actccttgcg atcgtgcctg atcttcagaa
      781 gtacgactcc aggaggatca ggcgtattgc ggcgagcgcc aagttcggag acgtcgccat
      841 cgtgcccgtc atcaacctgg ttgccagcgt tcctcggatt aaggagagca acctgttcga
      901 cgtcgatgcc ggacactttg cgcgtcagtt caaggatcag gtcgtcccgc tggcgatgca
      961 gttgatcgag ttcgccaacg acgtcatttt caccagcgac actctgcttg ccgcctcgtc
     1021 cgtacagggg cttcaggtgt accggttggc gcagcacgcc gtcaagcagc cggacggcgc
     1081 tgacttgcaa ccctttgtcg acgagatgtc gcacatcgtc aagaagacga tgaactacca
     1141 tagaaagccg gtaccggcac ccaccccttc cgccccgacg acgtctcaca ccccatccac
     1201 gcctggcgct cagggattcc tggcgtcgaa tctcgttccc gacaaaacca gcgagccaga
     1261 ggaagatgtc gcggaccatt tcgaacaggc gctgaaagac gtcaccaaga agccgtgagt
     1321 gatggggggc atgctgtcac gggcatgccc ctcgttcctt tgcgtctttg tggtgctaag
     1381 aagtccgcgc aggctgctcg aaccagtgct tcgttgacac gcacatgcgg cagacggaga
     1441 gcatcaccgg cacctcgacc aggacgccta cgacggtcgc gagcgcggcg ccggagccgg
     1501 ggccgtaaag cgcgatcgcg gtggctacgg ccagctcgaa gaagttcgac gctccgatga
     1561 gcgcgccggg agcggcgatc ggatacggga cgcggaacag cttcatcaaa ccgtacgtga
     1621 gcgacgagtt gaagtagacc tgcagaagga taggtacggc gatgaggaga acgtggaacc
     1681 agcgcgtggt gatgttctcc gcctggaacg cgaagatcag cacgagcgtc gccaggagcg
     1741 cgatgatcgt caccggcgcg aatcgcgtca tgagctcgcg ctcgaaccat tccgcaccct
     1801 tgcgccggat cagaacggcc cggctgatcg cgccgaggga tagggggatg acgacgaaga
     1861 tcaggacgga gtagagcaga acgcggaacg gcacttccag gccggccgcg ccggagatga
     1921 ggaacttcac gatcggcgca aaggcgacga gcatgatgag atcattcacc gatacctgca
     1981 ccagtgtgta tgccgggtcg ccgtcggaga gatacgacca gacgaacacc atcgccgtgc
     2041 aaggcgcggc ggcgagaata atcacgccgg cgatgtactg gctggccagc gcgggaccaa
     2101 taagcggaag aaagagatgc cggaagaaga gccagccgaa gaaggccatc gagaacggct
     2161 tcacgagcca gttgacgacg acggtgatga ggagcccttt cggcttacgc ccgacgcgca
     2221 cgatcgagcc gaagtcgatc ttgagcatca tcggatagat catcagccag atcagaatgg
     2281 cgatcgggat gttgatctgg ctgcccgtcc cgaactccat ccgctgcagg gcgcggacgg
     2341 aaccaggaag caacttgccg aggatcactc cggccaccat gcagagcgcg acccagaggg
     2401 ataggtagcg ctcgaagatg ttcattcgtt tcggtgctgc ggcgtccatc gatcttggga
     2461 acatcccgcg tacgaccgtt agtatattcg gataagcgca tgtcgttgcg cccgtcgcct
     2521 gtcttcgaac gatcaaaaca tgaaccacag agacacagag gttcacacga ttctctgcga
     2581 cctctgcgtc tctggggtga tatgagttct cgctcatgaa cggtttgatc ctcaactggt
     2641 tcctgtctgt tctcgtcgtc gcactaccgg gcgttgcggc cgagaaagac gacgcccaca
     2701 acatgcccgg catgcagatg cagagcgacg cgacgagcga gcttctgatg cgtcaggcgt
     2761 ccggcaccgg catgaatccc aacgccgctc cgatgcacat ggcgatgaca cagtacggcg
     2821 actggatgct gatgcttcac ggcctcgcct tcgtcaatca tgtcgtccag tccggcccgc
     2881 gcggcgatga caagctcttc tcgacaaact ggttcatggg aatggcggac cggcccctcg
     2941 gcggcggtca tctcatgctc cgttcgatgt tgagtctcga accgctgacg gtcggcaaga
     3001 agtaccccga gctgtttcaa accggcgaga cgatcggcgg acgtccgatc atcgacgccc
     3061 agcacccgca cgatttcttc atggagctcg ccgcggagta cgcgcatcca ttgacaggca
     3121 acacgatcgg ttacatctac gccgctccgt ttggcgatcc atcgctcgga ccggtcgcgt
     3181 atcctcatcg cgcctcggcg tcggagatcc cgcaggcggc gctcagccat cacgtgcagg
     3241 attcgacgca catcgccgga agcgtgctca ccatcggcgc gcaaagcggc atgttcggct
     3301 acgccttcag cggcttccac gggcgcgagc cggacgagaa gcgatgggac atcgacacag
     3361 gcaagatcga ttcgtgggcc gcgcgcttca cgttcgatcc gtcgcccaac tggaccgcac
     3421 agatttcgac cggccacctc aaacatcccg aagccgcgga gccgggcgac gtgcagcgaa
     3481 ccacggcatc gatcgcgtac agcgacgtta catctatcgg ccagtgggat acgagtctgg
     3541 tcttcggtca taacaacaaa agcgaaggac acgacggcag ttcgtggctg gcagaatccg
     3601 tattgcagtt cggcgggcgt aactacgtcg ccgcccgcgc cgaggccgtg gacaaggacg
     3661 agctgttcgc ggagcagaac gtgccgcccg ccatcgagca cggtctcttc cgcgtgaagg
     3721 ccctcacgct cggctattcg cgcgatcttc tcaccacgag cgaaatcacc ggcgccctcg
     3781 gcgggaacgt gacgttctac tcgttacccg acgcgatcaa gccgttctac ggcagcagtc
     3841 cgcactcggt gtacttcttc atcaggcttc gcggcggcag cgcaggtgcg cattcgatgt
     3901 gaggacgact tcaccacaga gacacagagg acacagagat tgaaagtggc ctctgtgtct
     3961 ctgtgcttcc agccgccgct ctagtcacgc gaagtccacg gatcgagaat ggcggttcct
     4021 gtcagtctcc cggcacgcaa atcggcgagc gcctcgttcg cgcgatcgag gggatagacc
     4081 gtgcgtttcg tctcgatgcg gagggaggcg atccgttgga agaactgatc gccgtcggcg
     4141 cgcgtaagat tagccacgga acggatggag cgttcgttcc acaacatctt gtaggggaac
     4201 gacgggatgt cgctcatatg aatgccggcg cagatcaccg tgccgccttt caccaggttc
     4261 cgaagcgccg tcacgacgag cgagccctcg ggagcgaaga tgatggcggc gtcgagtgga
     4321 tggggcggcg tgacgctgga gccagatgcc cagaccgcgc ccatgtcgcg cgcgaactgc
     4381 tgcgcggcgg aatcttcgtc gcgcgtgaag gcgtagacct cgcggccgtc agcgcgcgcg
     4441 atctgcacga ggatctgtgc agcggagccg aagccgaaga atccgatgcg tgcggcgtcg
     4501 ccggccattc gataggcgcg aaacccgatc agaccggcgc acaaaagagg ggccgcttcg
     4561 gcaggcgtgt agctctcagg aatccgaaaa cagtaacgtc ggtccgcgac ggcgtactgc
     4621 gcgtatccgc cgtcgacggt gtagccggtg aacttcgccc tgtcgcacag attctcgcgt
     4681 tcgctccggc aatacacgca aacaccgcac gtccaggcga gccacggaac accgaagcga
     4741 gtattcgtcc cttcctcgat cccgacgatc tggtgtccgg gaacgagcgg caacttcgga
     4801 tcgcgcagct cgccatcgac gacatgaaga tccgttcggc agacagcgca gcattcgacg
     4861 cggaggagca gttgctccgc gttcggttgc ggcaccggca gctcgatgag ctcgagcggc
     4921 ttgccagcct cgcgcaagac catcgcgtgc atcggggaag ggtacacggg gattcctcgg
     4981 gtgtgggcgt agtagaggag attcgtgttg cctaacccgg agttctcaca cttggattgc
     5041 ccggcagtga gcatccaaca aaggccagat gagggggcgc tgcgcaagca ctccatcact
     5101 gcaactggga cgatttcaag caggctcgga cgaggcgcga gttagtggcc tttgtgcatt
     5161 gattcccccc tcatccggct tcgccacctt ctccccccgc aaaaagcgcg gggggagaag
     5221 gcgctcgatg gaaaggattg gcggaaaaag gcagtggtgc aacggcgacg tgcatcaagg
     5281 gtttgcggaa tgtggttaac ccgctttttc taacacactc ttttcgcaac ctctccaacg
     5341 agcgccttct cccccacgct ttttgtgggg gagaaggtgg cgaagccgga tgagggggcg
     5401 ttgcacgagc actacatcag cgcatctgcc acgttttcag gcacgctcgg acgaggcgac
     5461 tggtcgcctt gcgcatttac tcccccctca tcctcgatgt cccggtcggt tcaggggaga
     5521 aggcgctcga ttggaaggga gtcgcggaag aagtactgtc gatgcgggcg cgtcacgcgg
     5581 acaagaacgg ccacagaggc tgcgcccagc tacggcttct tcacccttgc atccagaatc
     5641 gcgttcagca tctccgcggc cttgcctact tttggtccgg ggtgcgtgaa gaggttctcg
     5701 tcgacggcga cggcttccac gttctttgtt ccggcgcgat cgaggagggc gcggacttgt
     5761 tcgggggcga cggaatgatt tggatagagg agtaggtcgg gggggctggc gacgaatgat
     5821 tcgagggagt attgcggcca gccgttgacc tcgacggcgt tgcgtgcgcc gcatagtgcg
     5881 aagagatcgc cggcgaaggt gttgttgccg gcgacgtaga ggggatcggg ccagacggcg
     5941 agcatcacgc gcggcggctg cgcgcgcgtt cgtcttgcgc gagcgaggct ggcgttgagt
     6001 gcggcgacgg cgggcgtgct gtcgacgccg agggcagtgc cgatcgcggc catcgagcgg
     6061 gcaatgtcgg caaggcgctc gttcgtgacg acgagcagcg ggatgtggac cgcggcgagc
     6121 gcgcgcccga ggttgggagg gaggccggcg gcgttggcga tgacgaggtc ggggcggact
     6181 gccacgatct tttcgagatt cggctggata ccgccgacgt gcggaagctt gcgtccgggc
     6241 gtggcatccg cgatggcgtc ggcggagaag tcatcggtag cggtgatgcg gtctccggcg
     6301 ccgagggcga ggaccatctc cgtcacgttc ggcgcgaggg tgacgatccg gcgcggggtt
     6361 tgtttcggtg cgggctcgtt ggcggtgcac gcggccaaga gaacaagaag cacagacaag
     6421 agtgtctgtg ccacacagag acaagtctct gccacacaga aacaaaaacc gcggcgtcgt
     6481 ggccgcggtc tcggtgaatc gaatggagtc acgttctacg actcgccgcc gtcgtcatcg
     6541 tccatatcgt tgttgatgcc gaggcgggag caggcggcgg cgtacatctg cttcgaggca
     6601 ttgatcgcgt tctgcagctc ggcgatcctg tccttcacct tcgcaagctc ttcttcgatc
     6661 tggtctttca ggtcactgat ctcctgcttg gtaacctgaa gcgtcgtttc gtagaacttg
     6721 cgctgctgat ctgtgatgac cgtcatttct cctccaagtc agtggtgaac gggcagcgat
     6781 tatagcttga agtcgatgcg caccgtcgtc cacgacttca cgcggacgcc gtctttggtt
     6841 ccgggggtaa acgtcgagcg tttgattgat tcgatcgccg cagagttgag aatcggcgaa
     6901 gcgctgttga cgacgcgggt atcgatcacc tgtccgttct cgttcacgag cgcgctcacg
     6961 accaccgatc cctgcatgcg ttggacgcgg gcgatgggcg gatattgcgc cgctgcctga
     7021 cgcgctacgc gagccggatt cagtccctcg gttccttgcg agacgagatc gccgagctgc
     7081 gttcgtgggc tttcgggctg cacggccgga cgcgattccg ggacgggctg cggcaccgga
     7141 acgaccggtt gcggcgcagg cacgggacga ttctcgacga ccggaggcgg cactgcttgc
     7201 gccacctgcg gctgcggcgc cggagtcggt ggagcggttc ctcgattcgt cgtcgtggct
     7261 ggcgtgcttt gtgtgcgggc cagttgttcg agccgcgtct tctcggcggc catccgtttc
     7321 tgcacttctt cgttcacttt ggatgcatcg atcaccggcg cggtagcgac cgtcgacgtc
     7381 gatcctgtca ccggcgcgga tgtgcttgtg gacgtcgtcg tcgcgatcag gggcttcccg
     7441 gcgaccggaa ctttgcccgg tgcggcgacg gacgtcgtgg ccggctttcg catcatcagg
     7501 aaaccgacga tcaccgcgat cacgacggcg gcagcgatgg caatcggcac gatcggtggt
     7561 ttcttcgcgc tctccgctgc cgcgggtatc gcagcgggaa tcgctgccgg catgggcaag
     7621 ggcaccgcga cggcggccgg cttgattggc cgcagctcgt tcgcagcggg cgccggctcc
     7681 ggcatgggaa tcggctcgac gtgatgctgc tcaacgacgg cgctggaggc gaggcggtgc
     7741 atgtagatgg cgagatccgc gctcgtcggc gtgggccgga agttgtagag cactgcatcg
     7801 agatcgcgct gcatctcgcc ggccgtctgg taacgattgg cgggatcctt ctgcagcgct
     7861 ttgagcacga tggcgtcgat ctgcggcgtc acctcatcgt tcaactcgga cggtttggtg
     7921 acgcgcgctt cgcgcacctg ctcgaggatc gaaagctcgt tgtcgccgct gaagagtttg
     7981 cgagcggtga gcatctcgaa cagcacggtg gccagcgcga agatgtcgga gcggcggtcg
     8041 atgttgcgtc cccaggcctg ctcgggcgac atgtac
//
LOCUS       DATLVW010000768         4724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_568118, whole genome shotgun sequence.
ACCESSION   DATLVW010000768 DATLVW010000000
VERSION     DATLVW010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568118"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VNN25_22485"
     CDS             complement(<1..384)
                     /locus_tag="VNN25_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94362.1"
                     /translation="MRLRLGAVAVMCATSAFAQMRETVNVNVIEVPVSVVDSSGNPVR
                     GLTAANFEVYDNGKRQTISSFDKADFASKEVVNAISPMNPAARRSFLLLFDLGFSSPR
                     SLVRAQEAARSFVTTAVHPRDLVGVG"
     gene            555..1190
                     /locus_tag="VNN25_22490"
     CDS             555..1190
                     /locus_tag="VNN25_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94363.1"
                     /translation="MSLLMQRKGPAAPLPRLEGKEVLVGPNPDVAKVKGVMFGGRKQF
                     LADTAGEEGIATIIAKLSPKTASYTKTPLASSWCEFASLIELDRAIYETLKQKYPNVL
                     ALIGAASAELGIGRVYKSLDSTELLKFLENQAMFHSQFQKFGSVRFEKTATGGRMICS
                     DYPVYSPIYCASAAGFYLESILRHGGTDPGVIESKCQTLGDSNCTYEMTWR"
     gene            1230..2585
                     /locus_tag="VNN25_22495"
     CDS             1230..2585
                     /locus_tag="VNN25_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH94364.1"
                     /translation="MHFQQFYLGCLAHASYLIADGGEAAVVDPQRDVDQYLDAANALG
                     VTIRYILETHLHADFVSGHRELAERSGAEIVFGARANAAFPHRAVRDGDMLRIGNVAL
                     RAIETPGHTPESISWLVSEEGQPSKLLTGDTLFIGDVGRPDLAGARGHTPEEMASMLY
                     DSLHTKILTLPDDVEVWPAHGAGSACGRNMSKELTSTIGMQRKPNWALRPMPREEFIQ
                     ELTSGLAAPPRYFPLDAELNRVGPSSLSDEGPALLTADALPAGALILDVREAGAFGAR
                     HIDGAINIGLNGSFASWCGALLPFDAPIVIVADDADSAAQAVIRLARVGIERVAGYIA
                     AMDDYPTTPLAQLTVQELRDEAPAVLDVRRRGEFAESHVPGAKNIPLDELPDRLGEID
                     RDHPLAVICAGGYRSSIASSILARAGFTNVLNVQGGTEAWRRVSHHRDTESTEIKTET
                     L"
     gene            complement(2637..3476)
                     /locus_tag="VNN25_22500"
     CDS             complement(2637..3476)
                     /locus_tag="VNN25_22500"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HXH94365.1"
                     /translation="MTRQELLASIPLFESLTKEDLDGLTLHLEESHYEPGDVIFKQGD
                     DGSSLFIIEEGAVDIAFGEGPANIVLATLFNGQYFGELSLFDGAPRSATATATKHTHV
                     IRLDRDDLVKFVHKNPAASLRIIEEMGDRLRQTNELMSRQVSRNVLKEQEETLTFGQR
                     IADSVAAFGGSWPFIIMFSAIMIVWMTINVVSVVARFDPYPFILLNLALSSVAALQAP
                     IIMMSQNRQSTKDKLLAENDYQVNLKAEMEIAALLRGQSELLARLAVLERALEWRKSV
                     SSD"
     gene            complement(3564..>4724)
                     /locus_tag="VNN25_22505"
     CDS             complement(3564..>4724)
                     /locus_tag="VNN25_22505"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXH94366.1"
                     /translation="LFEWKPRSLIPVALASAVAAALRVHLLGAGPLFPVAAHGIVAWP
                     GLLAAVVVGLIAGAFSSGLSTLLYALEDAFEHLPLHWMWRPAVGGLVVGIGGYFQPRT
                     LGVGYDVIGDLLLGHLAVSIVLALLLVKCGIWLVSLASGTSGGVLAPLLIMGCGLGAV
                     EAIFLPSSGSASLWPLISMAAVLGGMMRAPFTAVIFALELTHDVESLPPLLIAAVVAY
                     GFTVLVMKRSILTEKVARRGYDIFREYTVDPLETYRVSEAMTESVLTIPESFPASEIY
                     ERYFSSSQKHRGFPVVDANDKLLGILTISDVMELSISEPDATLTARDLIRLDPIVAHP
                     EETCREAAERMAESGIGRLPVLSGGKLIGIITRSDLLKPRLLRFEEERRAPL"
BASE COUNT          965 a         1504 c         1410 g          845 t
ORIGIN      
        1 gccgacgccg acgagatcgc gcgggtgaac cgcggtggtg acgaagctgc gcgccgcctc
       61 ctgagcccgg accagtgacc gcggtgacga gaacccgagg tcgaagagaa gcagaaacga
      121 gcgccgcgcc gccggattca tcggcgagat ggcattcact acctctttcg aggcgaagtc
      181 cgccttgtcg aacgatgaga tcgtctgcct ctttccgttg tcatagacct cgaagttagc
      241 cgccgtgagg ccgcgcaccg gatttccact ggaatcgacg accgacaccg gcacctcgat
      301 gacgtttacg ttgaccgttt cgcgcatctg ggcgaacgcg gacgttgcgc acatcactgc
      361 cacggcaccg agacgcagcc tcatcgagtc ctccttggaa tggccggtta gcgacaccat
      421 ggccgaaaaa ccgcacccgg gtggttacag gacggtggcg tttgcttgta tcacgacgtt
      481 tgctgttggc cacggttgat gttgacgctg cactcgccac agcccaccgc cccacaccgc
      541 gatagaatca ctgcatgtct ctgctcatgc aacgcaaagg acccgccgct ccgcttccgc
      601 gactcgaagg aaaagaggtt ctcgtcggac ccaaccccga tgtcgccaag gtcaaaggag
      661 tcatgtttgg cgggcgcaag cagttcctgg ccgacaccgc cggtgaggaa ggaatcgcca
      721 ccatcatcgc gaagctctcc ccgaagacgg ccagctacac gaagactccg ctggcatcga
      781 gctggtgcga attcgcatcg ctgatcgagc tggatcgcgc catctacgaa acgttgaagc
      841 agaagtatcc caacgtgctg gcattgatcg gcgcggcttc agcggagctc ggaatcggac
      901 gcgtctacaa gtcgctcgac agcacggaac tgctgaagtt cctcgagaac caggccatgt
      961 tccacagtca gtttcagaag ttcggcagtg tgcgtttcga gaaaacagca accggcggcc
     1021 ggatgatctg cagcgactat ccggtctaca gccccatcta ctgcgccagc gccgccggct
     1081 tttacctcga gtcgatcctg cgccacggcg gaaccgatcc cggcgtcatc gaatccaaat
     1141 gccagaccct cggcgattcg aactgcacgt atgaaatgac gtggaggtag tcttcgcggc
     1201 cgtgcccggc taaaagaccc gcgaccccaa tgcacttcca acagttctac ctcggatgcc
     1261 tggcgcacgc gtcgtacctg atcgcagatg gtggtgaggc tgccgtcgtc gatccgcagc
     1321 gcgacgtcga tcagtatctc gacgccgcga acgcgctcgg cgtaaccatt cgctacatcc
     1381 tcgagacgca ccttcacgcc gacttcgtca gcggacatcg cgagctggct gagcgcagcg
     1441 gcgcggagat cgtgttcggt gcgagagcga atgctgcatt cccgcaccgc gccgtacgcg
     1501 acggcgacat gcttcgcatc ggaaacgtcg cgctgcgtgc gatcgagacg cccggccaca
     1561 cgccggagtc gatttcgtgg ctcgtttcag aggaaggcca gccatcgaaa ctgctgacgg
     1621 gcgacacgct tttcatcggc gacgtcgggc ggcctgatct cgccggcgcg cgcgggcata
     1681 cgcccgaaga gatggcctcg atgctctatg actcgctcca cacgaagata ctgacgctgc
     1741 ccgacgatgt ggaagtctgg cccgcgcacg gagcaggatc agcatgcggc cggaacatgt
     1801 cgaaggagct gacgtcgacg atcggcatgc agcggaagcc gaattgggcg ctgcggccga
     1861 tgcctcgcga ggagttcatt caggagctga cttcaggcct ggctgcacct ccgcgctatt
     1921 tccctctcga cgccgaactg aatcgcgtcg gaccctcttc gctgagcgac gaaggaccag
     1981 cgctgctcac cgcggatgcg ctgcctgccg gcgcgctgat cctcgacgtt cgtgaagccg
     2041 gcgcgttcgg tgcgcggcac atcgatggcg cgatcaacat cgggctcaat ggaagtttcg
     2101 cgtcgtggtg tggtgcattg ctgccgttcg atgcgccgat cgtcatcgtc gcggacgacg
     2161 cagactccgc cgcacaggcc gtgatccgcc tcgcccgcgt cggcatcgag cgtgtggccg
     2221 gatacatcgc cgcaatggac gattacccaa ccacgccact cgcgcaactc acggtacaag
     2281 agctgcgcga cgaggcgccc gccgttctcg atgttcgcag gcgcggcgag tttgcggagt
     2341 ctcacgttcc cggcgcgaag aacatcccac tcgatgagct tcccgatcgg ctgggtgaga
     2401 tcgatcgcga ccatcctcta gccgtgatct gcgccggagg ctaccgttcg tcgatcgcca
     2461 gcagcatcct cgcccgagcc gggttcacaa acgtgctgaa cgtgcaaggc gggacggaag
     2521 cgtggcggcg cgtttctcac cacagagaca cagagagcac agagataaag actgaaactc
     2581 tgtgatctct gcgcctctgt ggtgaagggc ttctctcgcc cttgatcttc aggccgctaa
     2641 tcactgctca ctgatttccg ccactccaac gcccgctcca agaccgccag ccgcgccagc
     2701 aactccgact gtcctcgcag aagtgctgcg atctccattt ctgccttgag attcacctga
     2761 tagtcgttct cggcgaggag cttgtccttc gtcgactgcc ggttctggct catcatgatg
     2821 atcggcgctt gcagcgcggc cacggaagaa agcgcgaggt tcaggagaat gaacggataa
     2881 ggatcgaacc tggccaccac actgacgacg ttgatcgtca tccagacgat catgatggcc
     2941 gagaacatga tgatgaacgg ccacgagccg ccgaatgccg caacgctgtc ggcgatacgc
     3001 tggccgaacg tcagtgtctc ttcctgttcc ttcaggacgt tgcgcgagac ctgccgcgac
     3061 atcaactcgt tggtctggcg cagccggtcg cccatctcct cgatgattcg aagcgatgcc
     3121 gcggggttct tgtgcacgaa cttcacaagg tcgtcgcggt cgagccggat gacgtgcgtg
     3181 tgcttggtgg cggtggcggt ggccgagcgc ggcgctccgt cgaagagcga cagctcaccg
     3241 aagtactgac cgttgaagag cgtggccaat acgatattgg ctggaccttc accgaaggcg
     3301 atgtcaaccg ctccctcttc gatgatgaac aacgacgatc catcgtcgcc ctgcttgaag
     3361 atcacgtcgc ctggttcgta gtgactctcc tcgaggtgaa gagtcaagcc gtcaagatcc
     3421 tctttcgtca gcgattcgaa gagcggaatg ctggccagca actcctgtcg tgtcatgaga
     3481 caagagagta cgtcagcgct ctccttcttc caagcgctga tcgcgtgcgc tgcgcagatc
     3541 gtcgcgcgtg cgtatgcgga cggctacaga ggggctctgc gctcctcttc gaatcgaaga
     3601 agccgaggct tcagcagatc gctgcgcgtg atgatgccga tgagttttcc acccgacaga
     3661 accggcagcc gtccgatccc tgattccgcc atgcgctcgg ccgcctcccg gcatgtctct
     3721 tccggatggg ccacgatcgg atcgagccga atcaggtcgc gggcagtcag ggtggcgtcg
     3781 ggctccgaga ttgacagctc catgacgtcg gagatcgtca agatcccgag aagcttgtca
     3841 ttcgcatcca ccaccggaaa tccgcggtgc ttctggctcg aagagaagta tcgctcgtag
     3901 atttcgctcg ccggaaagga ctccggaatc gtcaggaccg attcggtcat cgcctccgaa
     3961 accctgtacg tttccagcgg gtcgaccgtg tattcacgga agatgtcgta acccctgcga
     4021 gccactttct cagtgagaat ggagcgcttc atcacgagca cggtgaatcc gtacgcgacc
     4081 accgctgcga tcagcagagg aggcagcgac tccacgtcat gcgtcagctc gagcgcaaaa
     4141 atcaccgcgg taaatggggc gcgcatcatt ccccccagga ccgcggccat gctgatcaac
     4201 ggccagagcg aagcgctgcc ggaggatggc agaaagatcg cctcgactgc ccccagtccg
     4261 catcccatga tcaagagagg agcgagaact cctcccgagg taccggaagc gagagagacg
     4321 agccagatcc cgcacttcac gaggagcagg gccagaacga tcgaaacggc taaatgcccc
     4381 aggagcagat ctccgatgac gtcgtagccc actcccaggg tcctcggctg gaagtaacca
     4441 ccgattccaa cgaccagtcc acccactgcc ggacgccaca tccagtgcag aggcaaatgc
     4501 tcgaacgcat cttcgagcgc gtagagcagg gtgctgagtc ccgaagaaaa cgcgcctgcg
     4561 atcaggccga ccacaacggc cgcaagcaat ccgggccacg cgacgatgcc gtgagccgcc
     4621 acgggaaaca gagggccagc tccgagcaga tgcacgcgca acgccgcggc cactgcgctg
     4681 gccagtgcca caggaatcag gcttcggggt ttccactcga agag
//
LOCUS       DATLVW010000769         4355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_402405, whole genome shotgun sequence.
ACCESSION   DATLVW010000769 DATLVW010000000
VERSION     DATLVW010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402405"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1703..1996
                     /locus_tag="VNN25_22510"
     CDS             1703..1996
                     /locus_tag="VNN25_22510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94367.1"
                     /translation="MICSDVEMICSDIETIRSGIGMICSDIEMICSDIGVICSDVEMI
                     CSDIGMICSDIGMICSGIGMICSGIGMICSGIGTADCGIGTKHSDLLPPFPVF"
     gene            2335..3564
                     /locus_tag="VNN25_22515"
     CDS             2335..3564
                     /locus_tag="VNN25_22515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH94368.1"
                     /translation="MSGKRKKKLAVWSGIVLVIAIVITLVAMKAAGKKPEAKLPVKVG
                     KAEVADIQVKVTEVGNVQPEVKVDVKSVVSGKVVEILHRDGDSVKRGETLARIEPDMN
                     QAQSIADVKNALTASKIRYDQAKKNYESDHSLFESGLLAGKQNRDSEAEFLAAKQEYE
                     KTEEKYAIVERSGIPIAQATNKFQGSNVTAPMDGVVLTKNVEIGDTITSGVSSFNAGT
                     VLFSVADVSKMIVKAGVNEVDIGKIRVGMPVKVTLDAYSKIAFAGRIDRIAPAVRIDD
                     KVRVFDVEIRLDAQGRELRSGMTANIEVNGERKDKVLTVPVESVFQRDGVELVFVKKK
                     LDPNVVPTKDVKKDGQAPSAIDKDSWKKFFEKRTIVTGLSDNSRVEIVKGLKVGEEVA
                     LEDPTLPADKKKDENDD"
     gene            3686..>4355
                     /locus_tag="VNN25_22520"
     CDS             3686..>4355
                     /locus_tag="VNN25_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH94369.1"
                     /translation="MSALLQYEHPAAASAAQKALIQLDEICKTYDSGENAVQALRGVD
                     VAIEKGEFVSIIGPSGSGKSTLMHILGCLDTPTTGKYWLDGEDVAEMSARQLARVRNQ
                     KIGFVFQTFNLLPRASILKNVELPLLYAGASRDERRERAFKALQRVGLDKRAKHRPNE
                     LSGGQRQRVAIARALVNDPSLILADEPTGNLDQKTGSDIINIFEELAGHGETIVLVTH
                     DPAIA"
BASE COUNT          903 a         1318 c         1333 g          801 t
ORIGIN      
        1 attccggccg ccatcgccgg gacgagcatc gaagaggcat catattcgcc ggtagggtga
       61 cgagggggcg tccggcccgg ggtaagccgg ggacctaatg caagacggca cgccgccgct
      121 gcggagcgac ggtgttagta ggagccgtga aaacccacgc ggctcctgcg ttggtgttgt
      181 cctggtttcc acccgcgatt gccgttttgc cgtcggcaga gagagacaca gaactaccca
      241 ggaaagcctt cccaatagcg cccaagccga ccagcttgct tccctgttgt gtccaaactc
      301 ctccgctcct cgaccagacc caaattgcgc cggtgccgct gtggtcggtg aaccccgaga
      361 cgatggccgt gttgccatcg gcggatagtg acacggacca accttgttca gcggtcccaa
      421 gggagcccga gccgacaagc ttgtctccct gctgtgtcca gattcctccg ttcctgatcc
      481 atacccacac ggctccgact gacccgttgt ctccaagccc tccgacaatg gctgtgttgc
      541 cgtcggcgga aagtgacaca gaagatcctt gacctggacc aagcgcgctg cccgaaccga
      601 cgagcttgag tccttgctgg gtccagacac ttccgcttct catccacacc caagcagccc
      661 ctcggaagcc gttgtcgttc tgccctccga ccatggcggt attgccgtcg gcggataagg
      721 acaccgaggt accttgttcg gctgttcctg ccgcgtctga accaacaagc ttgtttccct
      781 gctgcgtcca gacgtcccca ctcctggtcc agacccatgc tgcgccggcc tgaccgtcat
      841 cggtccagcc tccgacgatc gccgtgttgc catcagcgga gaggcagacc gatttgcctt
      901 gttgcgcggc tcctacggcg cctgtgccga ccagtttcgt ttgttgagtc cacacgtttc
      961 ccgccctggt ccagacccac gcaccacccg taccactgtt gtctgcgcgg cctccgacaa
     1021 gggcagtatt accgtcggca gagatggatg cggagaagcc ttgttgagcc tttcccacgg
     1081 cgccagagcc cacgagcttg gcagactgct ctgtccagga tgcgccgttt ctcgtccaga
     1141 tccataccgc gccggtacct gagtcgtcgc cgctacctcc cacgatggcg gtgttgccgt
     1201 cacccgagat ggacacggat tggccctgaa aggcattccc gatcgcgcct gctcctacca
     1261 gcttcgctcc ctgctgcgag aactgcgcga gcgcgagatt ggaagagagg agtgaggtgc
     1321 aggcaaggac agtaagcaca aagggggggc gcattttcgt gggggatcct ctgaggagga
     1381 tggattgtat acgcccgaat ctcgaaaggc ggggccacga cctcgaaaca ggccgagggg
     1441 gtcagaccca aagtgaaagt gacggaaaat gcaccgcttt ttgtgggaga aggtggccaa
     1501 aggccggatg agggggcgtt ccaacgagca cgatatcagt gcatctgctg tgttttcagg
     1561 cagttcgcac gaggcttgcc cgagagtcgg cctacgttgt gcccgtcgcg cggccggagc
     1621 cgacgattgc gatatcggaa ccgagcattc cgacatcgaa accgttgctt tcgacatcga
     1681 aacgattcgt tccggcatcg gaatgatttg ttccgacgtc gaaatgattt gttccgacat
     1741 cgaaacgatt cgttccggca tcggaatgat ttgttccgac atcgaaatga tttgttccga
     1801 catcggagtg atttgttccg acgtcgaaat gatttgttcc gacatcggaa tgatctgttc
     1861 cgacatcgga atgatctgtt caggcatcgg aatgatttgt tccggcatcg gaatgatttg
     1921 ttccggcatc ggaacggcgg attgcggcat cggaacaaaa cattccgact tattaccgcc
     1981 ctttcccgtc ttttgacgcc tttctcacgc ttggtgcgcc tccttgctgg acctcttcct
     2041 tttctggtgt gcgattggaa agggactcgc cgaatggagc tcagtggacc gcgcgccggc
     2101 gcgagcagtc tgacccgtcg tgcagggcgc ggcgtcgccg atgtagtcgg tgccggcgga
     2161 cgacaccgga tcagtggcct gagtgatgag cacacgccga cgcgaggccg gatggatcta
     2221 caaggcgtgg agcacgagca cgcacgtcgc ggctcgggga gaggtgatca cccgctgaaa
     2281 ccttcgccct cctcggtcgt tatacgtggc agcaaacgaa ccgaggagcg tcctatgagc
     2341 ggcaagcgga agaaaaaact cgcggtgtgg tccggcatcg tcctggtgat cgccatcgtc
     2401 atcacactcg tggcgatgaa ggcggcgggg aagaagccgg aagcgaaact gccggtcaag
     2461 gtgggcaagg ctgaagttgc ggacattcag gtgaaggtca ccgaggtcgg caacgtgcag
     2521 cctgaggtca aggtggacgt gaagagcgtc gtgtcgggga aggtcgtcga gatcctccac
     2581 cgcgacggcg attcggtgaa gcgcggcgag acgctggcgc gcatcgagcc ggacatgaac
     2641 caggcgcagt cgatcgcgga cgtgaagaac gcgttgaccg cgtcgaagat ccgctacgac
     2701 caggccaaga agaactacga gtccgatcac agcctgttcg agtccggcct cctggccggc
     2761 aagcagaatc gcgattccga ggccgagttt ctggcggcga agcaggagta cgaaaagaca
     2821 gaagagaagt acgccatcgt cgagcgcagc ggcatcccca tcgcgcaggc cacgaacaag
     2881 ttccagggat cgaacgtcac cgcgccgatg gatggcgtgg tgctgacgaa gaacgttgag
     2941 atcggcgaca cgatcaccag cggcgtctcc tcattcaacg cagggacggt tctcttcagc
     3001 gtcgctgatg tctcgaagat gatcgtgaag gcgggtgtga acgaagtcga tatcggcaag
     3061 atccgcgtcg gcatgccggt caaggtgacg ttggacgcgt actcgaagat cgccttcgcc
     3121 gggcgcatcg accgcatcgc gcctgctgtg cgcatcgacg acaaggtccg cgtattcgac
     3181 gtcgagatcc gtctcgatgc ccagggccgc gagcttcgtt ccggtatgac cgcgaacatc
     3241 gaagtcaacg gcgagcgcaa agacaaagtg ctgaccgtgc ctgtcgagtc agtcttccag
     3301 cgcgacggcg tcgagctggt gttcgtgaag aaaaagctcg atccaaacgt cgtcccgacc
     3361 aaagatgtga agaaggacgg ccaggctccc tcggccatcg acaaagacag ctggaagaag
     3421 ttcttcgaga agcggacgat cgtgaccggc ctctccgaca actcgcgcgt cgagatcgtc
     3481 aaaggcttga aggtgggcga agaagtcgcg ctcgaagatc cgacactgcc tgcggacaag
     3541 aagaaggacg aaaacgacga ttagtgcttc ggggtgtggg gagtaggggg tgggaagtgg
     3601 agaaaacggc gagcacctta cggtcgtttt ttctaccaca ccccactccc cacaccccac
     3661 accccgcgag cgaagcgagc aaatcatgag cgcactcctc caatacgaac accccgccgc
     3721 agcatccgca gcgcagaaag ctctcatcca gctcgatgag atctgcaaga cctacgattc
     3781 cggcgagaac gcggtgcagg cgttgcgcgg cgtcgatgtg gccatcgaga agggtgagtt
     3841 cgtctcgatc atcggcccct ccggctccgg caaatcgacc ctcatgcaca tcctcggctg
     3901 cctcgacacc ccgacgaccg gcaagtactg gctggacggc gaagacgtcg ccgagatgtc
     3961 cgcgcgccag ctggcccgcg tacgtaacca gaagattggg ttcgtgtttc agacgttcaa
     4021 cctgctgccg cgcgcgtcga tcctcaagaa tgtcgagctg cccctgctgt acgcgggagc
     4081 gagccgcgac gaacggcgcg agcgggcatt caaggccctg cagcgcgttg gtctcgacaa
     4141 gcgcgccaag caccgtccga acgagctctc cggcggacag cgccagcgcg tcgccatcgc
     4201 ccgagcgctg gtcaacgatc cgtcgctgat cctggccgac gaaccaaccg gcaatctcga
     4261 ccagaagacc ggctccgaca tcatcaatat cttcgaagag ctggccggcc acggcgagac
     4321 gatcgtcctt gttacgcacg atccagcgat cgccg
//
LOCUS       DATLVW010000770        12144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_485379, whole genome shotgun sequence.
ACCESSION   DATLVW010000770 DATLVW010000000
VERSION     DATLVW010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485379"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VNN25_22525"
     CDS             <1..1093
                     /locus_tag="VNN25_22525"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH94370.1"
                     /translation="GWLVYKLTESPFLLGFVAFANSAPSLILMLPAGVLADHLDRRRV
                     VGISQIAQALSALFVAVAVRTGHITVWHIVAASAVSGVAISFSAPAYQAMIVDLLDDR
                     SRLSNAVAMNSLQFNFSRVLGPLLAGLTLAAWGSFWCFLLNALSFVPLVVVLERLKKR
                     QMRESPVGSMSTRLIEGFRYVRGDRLVLLLLGIVAVVSLLGYPYMTLMPMAARRLYVH
                     DDARGLGILMGAVGIGALLGALSLSVRTPTKIMRTIIASLTVFGVTLGCIGFTHARLI
                     TVPLLAICGCAMVVCVALCNTAIQNRIPDAMRGRVLSMYTFGFFGFLPFGNLLAGAVA
                     EHRGLPITMMMLGTGIIAAAVGAMVVRAS"
     gene            1214..2497
                     /locus_tag="VNN25_22530"
     CDS             1214..2497
                     /locus_tag="VNN25_22530"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HXH94371.1"
                     /translation="MIALLLLSVMALGAAPEATTGHPHFEIPRDTYLIDEAIPIVISG
                     LPPGQMVTVKLRSDDQPAWSSSASFIAGPDGVADLTRVAPSSGRYRGVDAMGLFWSAR
                     RDSSADRAPEAAPNPPPRKWQLIAEVRGVAVAETTVLRRAVAADVNVTAVRVNGLVGT
                     FYQPPGQERHPAVIVLPGSFGGVPPASAQAGGLASRGYAVLALAYFGAEGLPRSLSRI
                     PLEYFGKALDWLAAQPSVDPSRIGLLGISRGGELALLLGSAFPQIKSVVAYVPSNVVW
                     GGCCDSRNEPSWTVEGRPIAFVSTFGARDPVSMERATIKVERIKGAVLLISGRDDHVW
                     DSSGMSEKVMARLQRNQFPYPYKHLSCDGAGHAIGRPYTSTMDLNDTRHPLTGRLMQL
                     GGTPEGTARAREDSWRQVLGFLDEHLRNASPAANR"
     gene            complement(2500..3240)
                     /locus_tag="VNN25_22535"
     CDS             complement(2500..3240)
                     /locus_tag="VNN25_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HXH94372.1"
                     /translation="MTTTTTEPQTKPAATDAAAAPRQFVNFMFFKVDRAFRRETAAVR
                     AEAKAEFAEIVRKHTGPMMVLPYSTVGIKPNTDFMFWRIGYDIDPFQQMAAGINKSIL
                     GRYLDIPVSYFSMTKHSQYVDEHFHEGQEGRRLRIVPGKKPFLFVYPFVKTRDWYLLP
                     QSERQRIMNEHIAIGHKYPRVKINTTYSFGLDDQDFVVAFEADSAAEFLDLVQELRET
                     ESSKFTQRDTPMYTCRRSTVEEILDSLA"
     gene            complement(3695..4294)
                     /locus_tag="VNN25_22540"
     CDS             complement(3695..4294)
                     /locus_tag="VNN25_22540"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXH94373.1"
                     /translation="MKHWHVDGAPGNDVLDELAGILNAGGVALLPTDTIYGLHALAAN
                     QQAVDRIAAIKERPGDKAFVIIAASIEQLEAMKVTVPDALRTIWPAPLTAILPAGTST
                     IAARVPDLDWLRSLLERTGPLVSTSANRSGEPPVTSPEMLERTMQYRLDALLNQGPCE
                     GKPSTIVDFTESIGSTPRLVREGDPGFTQFLRKSLWKDL"
     gene            complement(4291..6012)
                     /gene="recN"
                     /locus_tag="VNN25_22545"
     CDS             complement(4291..6012)
                     /gene="recN"
                     /locus_tag="VNN25_22545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXH94374.1"
                     /translation="MITYLKVRNLAIVEEFAVEPGPGLNVLTGETGAGKSLLIDSIEF
                     LRGARSSTEMIRAGADKMTAEAVFHLPETSREALEALGIDVDGAEGGAVELIVKREIA
                     GNGRGRVLINGSPLSVRELGESMDAIVEIHGQHESHNRVAGQGYRELLDEYGDHTSLL
                     GSTGEAHREWRSAAEEFRALADAQRDRTLRLDLLRYQIDEIGAAKLAPAEEEGLRTER
                     SMLANARQLAEATSGAFALVDDDENSALAQLARALHLVQPLATEIADIGAIASEIEDA
                     VYRLQETSRGLSRLSDSVRHDPERLEEIEDRLVTVERLKKKYGGSVDAVLAYLETIQQ
                     EFERLSDYEANIDKLQRIEEQRFAAWRKTAESLSAARRKAAKKFETAIQLELNDLAME
                     RTTVKVVVDAAEPTREHAAAHGLDRIEILIAPNRGEEPKAMQRIASGGELSRIQLAIA
                     AALFKASRHAAAATLVFDEIDAGIGGRVAEVVGRKLQELAAGNQVICVTHLPQIASFG
                     STHFFVWKEDAAGHTRARIRRLDSDEERVNEIARMLGGESIPASALLHARELLDGARS
                     PAARSRR"
     gene            6107..7438
                     /locus_tag="VNN25_22550"
     CDS             6107..7438
                     /locus_tag="VNN25_22550"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXH94375.1"
                     /translation="MKTAGILAIAALTLAACNQEQVQQTTSRAQNAAQKVEQKVSDTF
                     DVSTPLGAKEPPMTPQEREKQRFDQQWRQLQSFRAQQAAQAAAAQQQQAAAQQAAAQQ
                     AAQTSTQLPPNVTIVHGQKESFKGLDANAINSAPVNLPISGDLRGPSVLKTQVYLDRN
                     HFSVGSIDGRWGRNSAITVWWWQKAHGMQPTGDVDDATFGSIARAAGGGQVVVPYTIT
                     ADDVKGPFVSIPDDPYEKAKLACLCYETLREELAEHFHCSEDFLEQLNPGVKINDLQP
                     GSTINVPNVRTPVTQDQPDIANIAISIAGNSYNGFDAGGNLVFHAPTTLGSGYDPSPD
                     ETVHVVEITPMPHFHYDPTLYHEVPDSEPDAHMNPGPNSPVGVVWMALSKPHYGMHGT
                     PDPDSIGYASSHGCVRLTNWDAQEVSHRIRKGVSVAFVDTTSKNMPDVAKK"
     gene            complement(7590..8876)
                     /locus_tag="VNN25_22555"
     CDS             complement(7590..8876)
                     /locus_tag="VNN25_22555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH94376.1"
                     /translation="MSSDLLNITRVVLPSGLTILIETLPYVRSVALGYYLRSGSAVES
                     EEHSGASHFLEHLVFKGTATRSTSDIAKVIDILGGDVDAFTGKEYTSFYCHVLDEQMM
                     TGLSLLTDIVISPRFTDDDLEVERGVILEEIKMVEDTPDDLVHEIFVTAFWPDHPLGR
                     PILGTEETVNRLGRKQIMAHYDETFHPSNMIFCASGNVRPEEIIPFLERTFASDTKAP
                     VARTWGAPTPGQHVILREKRELEQVHLCLGSRGFPQQQDERYAAALFNAILGGGMSSR
                     LFQKIREKAGLVYTVMSYHNGYVNGGYEAVYAACAPKNLKRVLDMTLAEMKLIKTGGA
                     TADELNSAKLHLKGSILLSLESTVSRMSGLARQEYYFGKQLSLDEIIAAIDNVSLDDI
                     ANVANTIVDPDSISLTLIGNLKSPGVTTDMLRGALA"
     gene            complement(9004..9675)
                     /locus_tag="VNN25_22560"
     CDS             complement(9004..9675)
                     /locus_tag="VNN25_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94377.1"
                     /translation="MPCAAARRRRDNTSESGFTLAAVIVLATIVMIFAAYTIPRAWST
                     IMKRERERQTIYAMQQYAKAIDEFRLKNGNAYPTSPQQIKDAKQPRLIRGLKGELVDP
                     LTGEVDWLVIPQSAAAALPQRNIGSNPNGPQNAPPPPPPPPPASASGTDTSGTNPQAP
                     PGLPGIPIKDYAGGPFIGVRPNAHGKSLTTLYGADTYETWVYTTVDYDAEKLLRIAQA
                     STVFH"
     gene            complement(9710..10147)
                     /locus_tag="VNN25_22565"
     CDS             complement(9710..10147)
                     /locus_tag="VNN25_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXH94378.1"
                     /translation="MKRPKSQSQSGFTLIELIVVVTIIGILAAVAISNVKWAQTKARE
                     AALHHDLFEMRKAIDDYYADKQKYPESLRTLLQEHYLRNLPPDPITGKTDWEEVQNTP
                     DPSDPNAIPTDPSAQADTAAPGIYDVHSQAPGNGLDGKPYKTW"
     gene            complement(10303..10794)
                     /locus_tag="VNN25_22570"
     CDS             complement(10303..10794)
                     /locus_tag="VNN25_22570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH94379.1"
                     /translation="MNGHRNKKSDGFTVAELITVIAIVAILATVAMPLANFGIRRQKE
                     IELHERLRRITEAIDRFHELALQQAGSPAAMKEPVSIEQGPWPKDLESLTKPIALNSG
                     QSVRLLRERDLIDPMTGRAEWNTLSADDDADTQSTNSHNVYEIHSKSTAMALDGKTHY
                     NEW"
     gene            complement(10794..>12144)
                     /locus_tag="VNN25_22575"
     CDS             complement(10794..>12144)
                     /locus_tag="VNN25_22575"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94380.1"
                     /translation="DSLRALKSANFLFTIPSISYNFVKSVGNTETLANPELRISEGEK
                     ATLHIGQRVPVPVTTFTTGVQSGSLGSLPATSFQYQDVGIKVSIEPRVHHNNEVTLKL
                     TVEVSDVAAAASADAPPAFNTRTIEATIRLKDGETNFLAGLIQDSNLTSSNKTPFLGD
                     IPILGRLFTKDHSTRHRTDLMLTMTPHIVRKPDITEDDLAPMWVGTQSNLSFRGLSPR
                     LESQSTADPFTPPPSSQQFRTGAVDSDGNPVQPQVVVPQGSAPTDPFKPPTPNAPPAN
                     PPTNPQPESRLSTPSTGTVVAQSVANGATNLTDNSGLAPRIQPQPMRMAFKPGEEKMW
                     NVVGMDLEGLSATQLLLHYNPQTLDVSEVVFGSALTIDLKTPPIATIDREHGTVRISS
                     SNGKPLQFNSGGGEIVTLRVRGGAPGETYLVLENPNLTNGGGTVVAASVSGGHAKVD"
BASE COUNT         2061 a         3624 c         3994 g         2465 t
ORIGIN      
        1 gggatggctc gtctacaagc tcacggagtc tccatttctc ctcggcttcg tggccttcgc
       61 caactcggcg ccgtcgttga tcctgatgct tccggccggc gttctcgccg accacctcga
      121 ccggcgccgc gtcgtcggca tctcgcaaat cgcgcaggcg ctgtccgcgc tgttcgtggc
      181 tgtcgccgtg cgcacgggtc acatcacggt ctggcacatc gtggcggcat cggctgtcag
      241 cggtgtggcc atttcgttct ctgcgccggc ctaccaggcg atgatcgtcg atctgctgga
      301 cgaccgttcc cgcctttcaa atgcggtggc aatgaactcg ctgcagttca acttttcgcg
      361 cgtcctcgga ccactgcttg cgggactcac gctcgcggcc tggggcagct tctggtgctt
      421 tcttctcaac gcgctttcgt tcgtgccgtt ggttgtcgtg ctcgaacggc tgaagaaacg
      481 gcagatgcgc gagtcgccgg tggggtcgat gtcgacgcgc ctcatcgaag ggttccgcta
      541 cgttcgcggc gatcgcctcg tgctgctgct cctcggaatc gtggccgtcg tcagcctgct
      601 cggctatccc tacatgacgt tgatgccgat ggccgcgcgg cgcctctacg ttcacgacga
      661 cgcccgcggc ctcggcatcc tgatgggcgc agtcgggatc ggcgcgctgc tcggagcgtt
      721 gtcgttgtcg gtgcgcacgc caacgaagat catgcgcacg atcattgcgt cgcttacggt
      781 cttcggggtc actctcggct gcatcggatt cacccacgcg cggctcatca ctgtgccgct
      841 gctggccatc tgcggctgcg ccatggtcgt ctgcgtcgcg ctctgcaaca ccgccatcca
      901 gaaccgcatc cccgacgcca tgcgcggccg cgtcctcagc atgtacacct tcggcttctt
      961 cggcttcctg ccgttcggga accttcttgc cggcgccgtc gccgagcacc gcggcttgcc
     1021 gatcacgatg atgatgctgg gcacgggaat catcgcggcc gcggttgggg cgatggtggt
     1081 tcgcgcatcc tgacaacgtt cactcacgcg aaggcgcgaa ggggtttggt accggtattc
     1141 ttcgcgccgt cgcgtcttcg cgtgaaagat cgagacgccc ggcactcccg gttttttctt
     1201 acaatggaat gccgtgatcg ctcttcttct cctatcggtg atggcgcttg gtgctgcgcc
     1261 cgaggcgacg accggacacc cgcacttcga gattccgcgg gacacgtacc tgatcgacga
     1321 agcgatcccg atcgtgattt cggggctgcc gcccggacaa atggtcaccg tgaagttgcg
     1381 aagtgacgat cagccggcgt ggtcatccag cgcaagcttc atcgccggac cggacggcgt
     1441 ggccgacctc acgcgagttg cgccgtcgag cggccgctac cgaggggtgg atgcgatggg
     1501 actgttctgg tcggcgcgcc gcgattcttc cgccgatcgc gcgccggaag cagcccccaa
     1561 ccctccgccg cggaagtggc agctcatcgc agaagtaaga ggtgtggccg tcgcggagac
     1621 caccgtgttg cggcgtgcag tcgcggccga tgtcaatgtg acggcggtgc gcgtgaatgg
     1681 tctggtgggg acgttctacc agccgcccgg ccaggaacgg catcccgccg tgatcgtgct
     1741 tcccggatcg tttggtggcg taccaccggc gagcgcacaa gcgggaggcc tggcgtcgcg
     1801 cgggtacgca gtactggctc tcgcatactt cggcgccgag gggttgcctc gctcgctctc
     1861 ccgcattcca ctcgaatact ttggcaaggc gctcgactgg ctggccgcac aaccttcggt
     1921 ggatccatcg cgcatcggtt tgctcggcat ctcgcgcggc ggcgagttgg ctctcctcct
     1981 cggatccgcc ttcccgcaga tcaagagcgt cgtggcatac gtgccgagca acgtcgtgtg
     2041 gggtggatgt tgtgactcca gaaatgagcc ttcatggaca gtcgaaggcc ggccgattgc
     2101 gtttgtcagc acattcggag cgcgcgatcc cgtgtcgatg gaacgggcaa cgatcaaggt
     2161 cgagcggatc aaaggggccg tcctgctcat ctctggtcgc gacgaccacg tgtgggattc
     2221 atcgggaatg tcggagaagg tgatggcacg actgcagcgc aaccagtttc cctacccgta
     2281 caaacatctc tcctgcgacg gcgccggaca cgccatcggc aggccatata cctcgacgat
     2341 ggacctcaac gacacgcgcc acccacttac aggccgactc atgcaattag gaggtacgcc
     2401 tgaggggacg gcacgcgcac gggaagactc gtggcgacag gtgctggggt ttctggatga
     2461 gcacctgcgc aacgcgagcc ccgctgcgaa tcgttgaggc tacgccaacg aatccagaat
     2521 ctcctccacc gtcgaccttc tgcacgtgta catcggcgtg tcgcgttggg tgaacttgga
     2581 ggactctgtt tcgcgcagtt cctgcacgag gtcgaggaac tcggcggcgg agtcggcttc
     2641 gaaggcgact acgaaatcct gatcgtcgag gccgaacgaa taggtcgtgt tgatcttgac
     2701 gcgtgggtat ttgtggccga tggcgatgtg ctcgttcatg atgcgctgac gttcgctttg
     2761 cgggagcagg taccagtcgc gcgtcttcac gaaggggtag acgaagagga acggcttctt
     2821 gccgggaacg atgcgcaggc ggcggccttc ctgcccttcg tggaaatgct cgtcgacgta
     2881 ctgcgagtgc ttcgtcatcg aaaagtacga gacggggatg tcgaggtagc gcccgaggat
     2941 cgacttgttg atgccggcgg ccatctgctg gaacggatcg atgtcgtagc caatgcgcca
     3001 gaacatgaag tcggtgttcg gtttgatgcc gacggtcgag taggggagca ccatcattgg
     3061 gccggtgtgc ttccggacga tttccgcgaa ctcggccttc gcctcggcac gcacggcagc
     3121 ggtctcgcgg cgaaacgcgc ggtcgacttt gaagaacatg aaattgacga actggcgcgg
     3181 cgcggctgcg gcgtcggttg ctgctggctt ggtctgcggc tcggtggtcg tggtggtcat
     3241 aatctcggat tctagcccgt gcgaggctcc gaggtgcact tcatcagtgt ctgcgcgtcc
     3301 tcgcgagacg agaggaatcg acgtcgcggt aaggcgttga gaccggacat tccgcggcga
     3361 tccagaggag gaacaggcgg cgaacacgaa aagctgattg ccgacgttta ctgacggcat
     3421 tacagcgtat ccaggcgaag cgagatgcac aagcgagcgc taaacccttt gtcacggttc
     3481 ggttaagcgg cgattaagca agagtccgcc ggtcactttt gtcaccggga gcatgtggaa
     3541 actgcaaaac gacttgggca actgcacaaa ctgcgctgct acggatgtgt ctgaaatcga
     3601 gtaaagctcg tagaacctta cacttgcgcg aattcgccca cgggtttgtg caaattgctc
     3661 agaagcccgt attctgcccg ctcttcgcag tttttcacag gtctttccac agacttttcc
     3721 gcagaaactg tgtaaagccc ggatcgccct cccgaacgag gcgcggcgtg ctcccgatgg
     3781 attctgtaaa gtccacgatc gtcgagggtt ttccttcgca gggcccctga ttgaggagcg
     3841 catcgagtct gtattgcatc gtacgctcga gcatttctgg cgaagtaacc ggcggttcgc
     3901 cgctgcgatt tgcgctcgtc gaaacgagcg ggcccgtacg ctccagcagc gaccgcagcc
     3961 aatcgagatc gggaactcgc gcggcaatcg tggatgtccc tgccgggaga atcgcagtca
     4021 gcggtgcagg ccagatcgta cggagagcat caggtacggt caccttcatc gcctccagtt
     4081 gttcgatcga tgcggcgatg atgacaaaag ccttgtcgcc gggcctctcc ttgatcgcag
     4141 cgatccggtc caccgcctgt tgattcgccg ccagggcatg gagaccgtaa atcgtatcgg
     4201 tcggcagtag cgccacgccg ccagcgttga gaatgccagc cagctcgtcg agaacgtcgt
     4261 tgcccggcgc gccatcgacg tgccagtgct tcatcgccgc gacctggccg cgggactgcg
     4321 cgcaccatcg agcagctcgc gcgcgtgcag gagcgccgag gctgggatcg actcgccgcc
     4381 gagcatgcgg gcgatctcgt tcacgcgctc ctcgtcggaa tcgaggcggc ggatgcgcgc
     4441 ccgcgtgtgg ccggcggcat cttccttcca gacgaaaaag tgtgtgctgc cgaaactggc
     4501 aatctgcggg aggtgggtta cgcagatgac ctgatttccc gccgccagct cctgcagctt
     4561 cctgccgacg acttcggcca cgcgcccgcc gatgccggca tcgatctcgt cgaagaccag
     4621 cgtggctgct gctgcgtggc gcgaagcctt gaacagcgct gcggcaatgg cgagctggat
     4681 gcgcgataac tcgccccccg atgcgatccg ctgcattgct ttcggttcct cgccgcggtt
     4741 cggcgcgatg aggatctcga tccggtctag gccgtgcgcc gcggcatgct cgcgcgtggg
     4801 ctcggcggcg tcgacgacga ccttgaccgt cgtccgctcc atcgccagat cgttcaactc
     4861 caactggatg gcggtctcga acttcttcgc cgccttcctg cgggcggccg atagcgactc
     4921 cgccgtcttg cgccaggcgg cgaaacgttg ctcctcgatt cgctgcagct tgtcgatgtt
     4981 cgcttcgtag tcgctgagcc gctcgaattc ctgctgaatc gtttcgaggt aggcgaggac
     5041 ggcgtcgacg gaaccgccgt actttttctt cagacgctcg acggtcacga gccggtcttc
     5101 gatctcttcg aggcgttcgg gatcgtggcg cacggaatcg gagaggcgcg agaggccgcg
     5161 ggaggtctcc tgaaggcgat agaccgcgtc ttcgatctcg gaagcaatcg caccgatgtc
     5221 tgcaatctcg gtcgcgagag gctgcacgag gtgtagagcc cgtgcgagct gtgccagcgc
     5281 cgagttctca tcatcatcga cgagtgcaaa cgcaccgctc gtcgcctcag caagctgacg
     5341 cgcattcgcg agcatgctcc gctcggtgcg caatccctct tcctcggctg gcgcaagttt
     5401 cgccgcgccg atctcgtcga tctggtagcg aagaaggtcg agccgcaacg tcctgtcacg
     5461 ctgggcgtca gcgagcgcac gaaactcttc cgcggcactg cgccactcgc ggtgagcttc
     5521 gcccgtgctg ccgagaagcg aggtgtggtc gccgtactca tcgagcagtt cgcggtaacc
     5581 ctgaccggcc acgcggttgt gcgattcatg ctggccgtgg atctcgacga tcgcgtccat
     5641 cgactcgccc agctcgcgga cggagagggg cgagccgttg atgaggaccc gtccacgtcc
     5701 gttacccgcg atctcgcgct tgacgatcaa ctcgacggcg ccgccctctg ctccgtcgac
     5761 gtcaattccc agcgcttcga gtgcctcgcg cgatgtttcg ggcagatgga acacggcctc
     5821 ggcggtcatc ttgtccgcgc ccgcgcggat catctccgtc gagctgcgcg ccccgcgcag
     5881 gaactcgatc gagtcgatga gcagcgactt tccggcgccc gtctcgccgg tcagaacgtt
     5941 cagtcccgga cccggctcga cggcgaattc ctccacgatg gcgaggttgc ggactttgag
     6001 ataggtgatc accggacgtt tggaaagtct accgcgttca ccgggagcgg cgcacttcgc
     6061 ggtgggcagt gactttgcta ttgctatctg cgaatgaggg gtgaccatga agacggctgg
     6121 cattcttgcg attgcggcgc tgacgttggc ggcgtgcaat caggaacagg ttcaacagac
     6181 gacctcgcgc gcgcagaacg cggcacagaa ggttgagcag aaggtatccg atacattcga
     6241 tgtgtcgacg ccgctcggcg cgaaagagcc gccgatgacg ccgcaggaac gcgagaaaca
     6301 gcgtttcgat cagcagtggc gccaactgca atcgtttcgc gcgcagcagg ccgcgcaggc
     6361 cgccgctgcc cagcaacagc aggcggcggc acaacaggca gcggcccagc aggccgcgca
     6421 aacctcaacg cagttgccgc cgaacgtcac cattgttcac gggcagaaag aatcgttcaa
     6481 gggtctcgat gccaatgcga tcaacagcgc accggtcaat ctgccgatca gcggcgacct
     6541 tcgcggccct tccgtcctga agacgcaggt ctatctcgat cggaatcact tttcggtcgg
     6601 ttccatcgac ggccgctggg gccgtaattc ggcgatcacc gtttggtggt ggcagaaggc
     6661 gcacggaatg caaccgaccg gcgatgtcga cgatgccacg ttcggctcga tcgcgcgcgc
     6721 ggcgggtggc ggacaagtag ttgttccgta cacgattacc gccgatgacg tgaagggacc
     6781 attcgtcagc atccccgacg atccttacga gaaagcgaag ctcgcgtgtc tctgctacga
     6841 gacgctccgc gaagaactgg cggagcattt tcattgctcc gaagacttcc tcgagcagtt
     6901 gaaccccggc gtgaagatca atgatctcca gccgggatcg acgatcaacg ttccgaacgt
     6961 ccgtacgccg gtgacgcagg atcagccgga catcgccaac atcgccatct ccatcgcagg
     7021 caattcgtac aacggcttcg acgccggcgg caatctggtc tttcacgcgc cgacgacgct
     7081 cggatcggga tacgacccgt cgccggacga aacggtgcat gtcgtcgaga tcacgccgat
     7141 gccgcacttt cactacgatc cgacgctcta tcacgaggtc cccgacagcg agccggacgc
     7201 gcatatgaat ccgggcccca actcgccggt aggcgtcgtc tggatggcgc tgagcaagcc
     7261 ccactacgga atgcacggca cccccgatcc cgactcgatc ggctacgcgt cttcccacgg
     7321 ctgcgtacgc ctgaccaact gggacgcgca ggaagtctcg caccgcatca ggaaaggagt
     7381 atcggtagcc ttcgtcgata cgacctcgaa gaacatgccg gatgtggcga agaagtagga
     7441 gtgcggaggt ctcgtccgcg gcatttcggg gcgtcatcgc cccgaatgtc tattctcgcg
     7501 aagatcccgg aggagtagcg cgttctcgga gccgaacaag cactcgggcg aggacgcccg
     7561 agtgcccgcg agcgagacgc tcgcactcct caggccaacg cgccgcgcag catgtcggtg
     7621 gtgactccgg gcgatttcag gttgccgatc agggtgagtg agatcgagtc ggggtcgacg
     7681 atcgtgttgg cgacgttggc gatgtcgtcg agggacacgt tgtcgatcgc ggcgatgatc
     7741 tcgtcgagcg atagctgctt gccgaagtag tactcctggc gggccagtcc ggacatgcgg
     7801 ctcacggtcg actcgagcga gagaaggatc gatcctttga gatggagctt ggcggaattg
     7861 agctcgtccg ccgtcgcgcc accggtcttg atcagcttca tttcggccag ggtcatgtcg
     7921 agcacgcgct tcaggttctt gggggcgcag gcggcgtaga ccgcttcgta gccgccgttg
     7981 acgtagccgt tgtggtacga catcacggtg tagacaaggc cggccttctc gcggattttc
     8041 tggaagaggc gtgaggacat cccgccgccg aggatggcgt tgaaaagggc ggcggcgtag
     8101 cgctcgtcct gctgctgggg gaacccgcgc gagccgaggc agaggtgaac ctgctccagc
     8161 tcccgtttct cgcggaggat cacgtgttgt cccggcgtgg gcgctcccca cgtgcgcgcg
     8221 accggcgcct tggtgtcgct ggcaaacgtt ctttccagga acgggatgat ctcctcggga
     8281 cgcacattgc ctgacgcgca gaagatcatg ttcgacggat gaaaggtctc gtcgtagtgg
     8341 gccatgatct gctttcgtcc gagccggttc accgtctcct cggtgcccag aatcggccgt
     8401 ccgaggggat gatcgggcca gaaggcggtc acgaagatct cgtgcacgag gtcgtcgggc
     8461 gtgtcttcga ccatcttgat ctcttcgagg atgacgccgc gctcgacttc gagatcgtca
     8521 tcggtgaagc gcggcgagat gacgatgtcg gtcaggagcg agagaccggt catcatctgc
     8581 tcgtcgagga cgtggcagta gaaggacgtg tactccttgc cggtgaaggc gtcgacgtcg
     8641 ccaccgagga tatcgatgac cttggcgatg tcgctcgtcg acctggtggc cgttcccttg
     8701 aagacaaggt gctcgaggaa gtgcgacgcg ccgctgtgct cctccgactc gacggcggag
     8761 ccgctgcgga ggtaatagcc gagcgccacg gagcggacgt aggggagcgt ttcgatcagg
     8821 atcgtcagcc cggaggggag gacgacgcga gtgatgttca gtaggtcgga tgacatgcga
     8881 gcgggattgt agtcttgggc gggggtcgcg ctgcgggtgt cgcgagtcgg gggtcggggg
     8941 tcggagcaag cggcgggtcg tcgatccatc tccgactccc gactcccgat tcccgacaac
     9001 cgctcagtgg aagaccgtgc tcgcctgggc gatgcgcaac agcttctccg cgtcgtagtc
     9061 gaccgtcgtg taaacccagg tctcgtacgt gtcggcgccg tacagcgtcg taagcgattt
     9121 gccgtgcgcg ttggggcgga cgccgatgaa cggaccgcct gcgtagtctt tgatcggaat
     9181 gccgggcagt ccgggaggcg cctggggatt ggtaccgctc gtatcggttc ccgatgcgga
     9241 cgcgggtggt ggcggcggtg gcggtggcgg ggcattctgt gggccgttcg gattgctgcc
     9301 gatgttgcgc tggggaagcg ccgccgcagc cgattgtggg atgacgagcc aatcgacttc
     9361 gcccgtgagc ggatccacga gctcgccctt cagcccgcgg atcaagcgcg gctgcttggc
     9421 atcttttatt tgttgcggcg acgtggggta ggcgtttccg ttctttaagc ggaactcgtc
     9481 gatcgccttc gcgtactgct gcatcgcgta gatcgtctgg cgttcccgct cccgcttcat
     9541 gatcgtcgac caggcgcgcg gaatcgtgta ggcggcgaag atcatgacga tcgtcgcgag
     9601 aacgatgacg gcggcgagag tgaagccgga ttcggacgtg ttgtcgcgcc gccgtcgggc
     9661 cgcagcgcag ggcacttgac gatcagcctg ggacgattgc ggcctcatct caccaggtct
     9721 tgtacggttt gccatccagc ccgttgccgg gcgcctggct gtgtacgtcg tagattccgg
     9781 gggcagcggt gtcggcctgc gcggacggat cggtgggaat ggcattgggg tccgatggat
     9841 cgggcgtgtt ctgcacttcc tcccaatccg tcttgccggt gatcggatca ggcggaagat
     9901 tgcggagata gtgctcctgc agaagcgttc gcaggctttc gggatacttc tgtttgtccg
     9961 cgtagtagtc gtcgatcgct ttgcgcatct cgaagaggtc gtggtgcagc gctgcttcgc
    10021 gagccttggt ctgtgcccac ttgacgttcg agatggcaac agccgcgagg atgccgatga
    10081 tcgtgacgac gacgatcagc tcgatgaggg tgaagccgga ctgggattgt gatttgggtc
    10141 gtttcatgtg tttgtccaga gagatcagag attagagatc agagatcagt ggattagctg
    10201 attccggcag tcggcgaatc tactaatcgc taatcgccag gcctctagtc gctagtctct
    10261 gatttctagt ctctgatctc taatcaactg atccactatc cttcaccatt cgttgtaatg
    10321 cgttttccca tcaagggcca tcgcggttga cttggagtga atctcgtaaa cgttatgact
    10381 gttcgtcgac tgcgtatcgg cgtcatcgtc cgcggagagc gtgttccatt ccgcgcggcc
    10441 ggtcatcgga tcgatcaggt cgcgctcgcg caggagccgc acggactgcc cgctgttcag
    10501 cgcgatcggt ttggtcagcg actcgaggtc tttgggccac ggaccctgtt cgatgctaac
    10561 cggctctttc atcgctgcgg gactgccggc ttgctgcaac gccagctcgt ggaagcggtc
    10621 gatggcttcg gtgatccggc gcagacgttc gtgcagttcg atctctttct gccgccggat
    10681 gccgaagttc gcgagcggca tggctaccgt cgcaaggatg gccacgatcg cgatgacggt
    10741 gatcagttcc gcgaccgtga atccatccga cttcttgttg cggtggccat tcatcagtcg
    10801 accttggcgt ggcctcctga gacggatgcg gccacgacgg tgccgccccc gtttgtcagg
    10861 ttgggattct cgagtacgag ataggtttcg cccggggctc ccccgcggac gcggagcgtc
    10921 acgatctcac cgccgccgct gttgaactgg agaggcttac cgttcgatga cgagatgcgc
    10981 acggttccgt gctcgcgatc gattgtggcg atgggtggtg tcttcagatc gatcgtcagg
    11041 gcggagccga aaacgacctc gctgacatcg agcgtctgcg ggttgtagtg aagcagaagt
    11101 tgcgtcgccg acagaccttc gaggtccatt ccgacgacgt tccacatctt ctcttcgcct
    11161 ggtttgaagg ccatccgcat cggttgcggc tggatgcgtg gcgcgagccc gctgttgtcg
    11221 gtcagattgg tggcgccgtt tgcgaccgac tgggcgacga ctgtgccggt ggacggggtc
    11281 gacagccgac tctccggctg cggattcgtc ggtggatttg ccggcggcgc gttcggcgtc
    11341 gggggcttga agggatctgt cggagcggag ccctgcggga cgacgacttg cggctgaacc
    11401 gggttgccat ccgaatcgac cgcgccggtg cggaattgct gcgagcttgg cggcggcgtg
    11461 aacggatcgg cggtggactg cgattcgaga cgcggcgaga gtccgcggaa gctgagattg
    11521 ctctgtgttc ccacccacat cggggcgagg tcatcctcgg tgatgtcggg cttgcggacg
    11581 atgtgaggcg tcatcgtcag catcaggtcg gtgcgatgcc gcgtggagtg atccttcgtg
    11641 aagagccggc cgaggatggg gatgtcgccc aggaacggtg tcttgttgct cgaagtcagg
    11701 ttcgagtcct gaatcagtcc tgccaggaag ttcgtctcgc cgtctttgag gcggatcgtt
    11761 gcttcaatcg tgcgcgtgtt gaatgcaggc ggagcatcgg cgctggcggc ggcggcgacg
    11821 tcggaaacct cgacggtgag cttgagcgtc acttcgttat tgtggtgtac gcgcggctcg
    11881 atcgagactt tgatgccgac gtcctggtac tggaacgacg tggccggaag gcttcccagc
    11941 gatccgctct gcacgccggt ggtgaacgtc gtgaccggaa caggaacgcg ctggccgatg
    12001 tggagcgtgg ccttctctcc ctcggagatg cggagctcgg ggttcgcaag cgtctcggtg
    12061 ttgccgacgc tcttgacgaa gttgtaggag atcgacggga tcgtgaagag gaaattggca
    12121 ctcttcaggg cgcggagtga atcg
//
LOCUS       DATLVW010000771         9846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_76440, whole genome shotgun sequence.
ACCESSION   DATLVW010000771 DATLVW010000000
VERSION     DATLVW010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76440"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1320)
                     /locus_tag="VNN25_22580"
     CDS             complement(73..1320)
                     /locus_tag="VNN25_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94381.1"
                     /translation="MKKILLSLLLLHTVTAFAQPTVSPEVASDPIPGRASVSSFAIPA
                     LAMARDRKGLAVAWTAQGPDGRDQIAVVRLDGSAHFAGPVRMIPAASLVSVDGADPSI
                     AAAPGGDGFVLVWIERPSNGAVVAYSRLDADLNPSVPVIVPSRNGFSISAPPIVRSGK
                     TTWLTADTWVWQIRADGSLSGPFDGGIPGSDMAVTTDLPQLVSGITGTSDTFTCKSEP
                     GCTVGGGPFRGYCYENCRINGYSYGLSFTALYTVSAATRFALNTDTKPAIRSDGHDAL
                     MAWFLGTESNGGLLVSARLNPASFTQFSQAANDHRVLATLGPDAGASRADIASDDDRY
                     VVVWRTKSPTHDHDIAGASVDRSGNIIAFPIATSPDDERDPSVIAIGSGTFLVAYEKF
                     NSAGERRIAGRLVTFGSRSHAVR"
     gene            1401..3266
                     /locus_tag="VNN25_22585"
     CDS             1401..3266
                     /locus_tag="VNN25_22585"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXH94382.1"
                     /translation="MRKWWVGLLGALLLPAALAAASSSKILAVANVTPELRASLTSDY
                     RLDIVVKPHVGDAWSRLAKRVTGDGARWNEIASFNQAGGNLTTEQSVRIPFNILRPNL
                     QHDVVAMLFPSDSAVDSGWRHVVVGSSGIEGESLWNIAEWFTGSGENYAAIRAANPSQ
                     GLSTRKGDVILIPKELLASAFRRGEMEERYAPKTAAVRKSEDDPEERTSTDAHGAAAP
                     LPETVALGESAQPSLTPSLTYDRTAAEPFAVYRLQKGEALYSSVAIRFTGRVYAKDVG
                     DVLERIERFNGIDDVARIPIGYRVKIPMSLLLPEYLPADDPTRVASEQSKRESEMLAV
                     RPRAKGLAGVRVILDAGHGGRDAGATYDDVWESNYVYDVMCRLKRILEKKSGATVSAT
                     TKSKEAGYAIPDGDELEEQTDHIILTSPKYVIGDPAVGVNLRWYLANSIFRRAMKQSV
                     PKEKVVFLSIHADSLHPSLRGAMAYIPGQRYVTGSYEKSDQVYLARAEVRESPVVRHS
                     EKESLAAEGLSRDLAESIIDAFETDDLKVHPFHPVRDNVVRDGREWVPAVIRYNLVPT
                     RVLLEICNLGNEKDRALIKTKKYRQHIAEAIYEGLVNYYNDRDDVRGAPSIASGR"
     gene            3383..3862
                     /gene="ribH"
                     /locus_tag="VNN25_22590"
     CDS             3383..3862
                     /gene="ribH"
                     /locus_tag="VNN25_22590"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXH94383.1"
                     /translation="MPRSFEGDHNAEGLRFAVIASRFNDEIVDGLLRGALECLARHGA
                     ADDDISVYRVPGAFEIPTLARMLIDRGGIDAVITLGCLLRGDTPHFDFISSQVTNELS
                     HVAVKTRFPVAFGVITCNTREQAEQRSSPGSGNKGWEAALAAVEMANLWREINSGEF"
     gene            3862..4275
                     /gene="nusB"
                     /locus_tag="VNN25_22595"
     CDS             3862..4275
                     /gene="nusB"
                     /locus_tag="VNN25_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXH94384.1"
                     /translation="MGARRKARELVLQMLYQHDMSGNAPDMIVDTFDDLQKSKPNTRD
                     FATKIFRGTVDNLFEIDGMIQAQADNWRLSRMAVVDRNIIRMSIYEFLHEAGTPKLVV
                     IDEAIEIAKKFGTEKSSQFINGILDGILKRYNLAS"
     gene            4373..6247
                     /locus_tag="VNN25_22600"
     CDS             4373..6247
                     /locus_tag="VNN25_22600"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94385.1"
                     /translation="MSLLGRLEDLSLTDIVQIVYLSRRTGVLEILDEHGRHTVLFRHG
                     LVVNASSPEHPDLTSYLAAKGLVPEAAVPGLRQMEEKGIPCGTAAIEMNFLTAADLAT
                     AVHERIVSVVAPLLQSREGEFNFILSDHVGPLDVEYDADAIFKEGGFPPQKIVGAADG
                     EKLKPLRGLEESMKVGKALLRGSASPEAPAPSLDLGLGQAKPPALSVVIEDNVLPFPT
                     PEERDDATFAPVPDDEPFPDVAPAPELKPAPEIEEPKLRPVPPEPAPAPPEQAPLSRA
                     SQFKVAGGLIEVESPDATYRNVVLFERNPLIRVAARRAFGRNGVKIAQFGSLDDARVA
                     IGDYIRANTFFVTFLELSADAEVLMQQLKRKNPRLPVVVIDAEPDLRRRHDLLRKGAD
                     LYLTKPSAARLQPGLAEEELSLFADELVLFAERAFQQWEQFTGGLDPDAGKRFYETAE
                     KESVDRSFGLLKQLINELSNPNDIGEVSATILRLSAEYLDRGAIFIAGDHMFTGVAGF
                     GPTDGGEDMTKRIKSLKLPRAVPSILSDVASSGEAHRGKMRRTPANVELINSLGGLLP
                     SEVVALPIMHAKRTIGILYGDNAAHRAPIDAMTGLEIFLSQAGYAFGNAVFAAEKAGR
                     "
     gene            6244..6621
                     /locus_tag="VNN25_22605"
     CDS             6244..6621
                     /locus_tag="VNN25_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94386.1"
                     /translation="MKVLVVEDSSSMRAYLTTIIEGGTEAYDLEIVEAASGFEALKTL
                     PHHKFDAILTDINMPDINGLELVSFLKNHPVHRSIPIMVISTESRDEDRKRAAALGAE
                     EYLVKPFQASELIEKLRRLLKVA"
     gene            6721..8769
                     /locus_tag="VNN25_22610"
     CDS             6721..8769
                     /locus_tag="VNN25_22610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXH94387.1"
                     /translation="MSKEDDRALNEFTGEAEELLDTLSRDLVELEAQGRDVRPEVINK
                     IFREVHSLKGLAGMLGFGEISELSHTLEDMLDRLRMGKIEITRDLIDLLYDAVDSLNR
                     LVIAINDPAVSGLIDITGLTRRIHHVISSQPQKAHDDPFADLTLDEQTKKSLTEYEEH
                     RLVENVRGRKHILAVEVRFDFSDFDEKLRALTELLSQSGEVISTLPAIDSSGGAGIAF
                     RLLYGSTMDVDEVAALVPEGSVTSLRVAELPGRSQPADAAAPIEIPEEDLSLRSISQT
                     VRVDISKLDHVMNIVGELIIEKTQLDALTRSLHQGEALQQARLTAHELTKIARNFDRK
                     LNELQKSVIETRMVPVGQIYAKLSRTVRKVARELNKEIELVLRGEDTELDKVMVEELT
                     DPLMHIIRNALDHGIESAEERVKAGKNPIGRITLNAYQQGNSVVLDVTDDGRGIDPEN
                     VRKAAIKRNLISQHDPFDQVRAYETLFTPGFSTASAVSEISGRGVGLDVVKKNLQELK
                     GTIDIRSVPGEGSTFRIMLPITLAIIQALVVKAGGEQFAIPLTSVEESLRIYSRDIRT
                     VERREVFTLRDFTLPLLRLADAFHLGHERDENPDSKWFVVVTRSGEKVAGILVDALVR
                     QQEVVIKSIGERLKTIPGIAGATEVGEGEIVLVIDVGTLIEQFGGKAREMRAAVAWVT
                     "
     gene            8793..9638
                     /locus_tag="VNN25_22615"
     CDS             8793..9638
                     /locus_tag="VNN25_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH94388.1"
                     /translation="MFEEFFGFTTKPFGKTPDPGFLYESPQHSEALARLEYAVEEKEL
                     SLLVGDIGSGKTTLSRALIDRIGESRPVILLINPRLTPVQLLRSIASGLGIQPARYRN
                     DLLDQIHAKLFELYEAGREPVLIIDEAQLIPSKATFDEIRLLTNFQLDDQNLLSVLLI
                     GQPELEVRLERSHYAPLRQRIGMRYRLGPLSLEETTRYVEHRIRVAGGTRNPFSEDAV
                     KAMHAVSGGIPRVINTLATTALLDAFGDDAPTIDASRIAAAAREHRLREAPPVNGDRI
                     EQPHG"
     gene            9631..>9846
                     /locus_tag="VNN25_22620"
     CDS             9631..>9846
                     /locus_tag="VNN25_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94389.1"
                     /translation="MVDLVKLRKKAKGARAAGDASAEKRPDVGSRESEMVPAEVPAPT
                     PDRRPPALLEPSTLSQSERPTIKKPAPT"
BASE COUNT         1948 a         3126 c         3051 g         1721 t
ORIGIN      
        1 gaggattgtg ggcgtcccgc ctcggtggtt ggatccccgg cctgagagcg agaatggttt
       61 gaaactagcc agctaccgca ccgcgtgcga cctcgacccg aacgtcacca atcgtccggc
      121 gatacggcgt tcgcccgcgg agttgaattt ctcgtaggcc acgagaaacg ttccgctgcc
      181 gattgcgatg accgaggggt cgcgctcgtc gtccggtgag gtggcgatcg ggaacgcgat
      241 gatgtttcca ctgcgatcga ccgacgcgcc ggcgatgtcg tgatcatgag tcggagactt
      301 ggtgcgccag acgacgacgt agcggtcgtc atcgctggcg atgtcggccc gcgacgcacc
      361 cgcatcgggt ccaagggtcg ccagaacacg gtggtcgttt gcggcttgcg agaactgggt
      421 gaaacttgct gggttaagac gagcggacac caggagtccg ccgtttgact ccgttccgag
      481 gaaccaggcc atcagcgcgt cgtgtccatc gcttcgaatg gccggcttcg tgtcggtgtt
      541 gagcgcgaac ctcgttgccg cagagacagt gtagagggca gtgaaactca gcccatacga
      601 atacccgttg atcctgcagt tttcgtagca gtagcctcgg aacggtcccc cgccgacggt
      661 gcagcccggc tcagacttgc acgtaaacgt atcggaagtc cccgtaatgc cggaaaccaa
      721 ctgcggcaag tccgtcgtca ccgccatgtc gctgcccggg atgccgccat cgaagggacc
      781 gctcagtgag ccgtccgccc ggatttgcca gacccaggta tcggcggtga gccatgtggt
      841 tttcccagag cgcacgatcg gcggcgcgct gatcgaaaag ccgttgcgcg agggaacgat
      901 gaccggtacc gacggattca ggtccgcgtc cagccggctg taggcgacaa cagctccgtt
      961 ggatggccgc tcgatccaga ccaggacgaa accgtctccg ccgggagcgg ctgcgatgga
     1021 agggtcggcg ccatcgacgg aaaccagcga tgccgctgga atcatgcgca ccggacccgc
     1081 gaagtgagcc gagccatcaa gccggacgac ggcgatctgg tctcgtccat caggtccctg
     1141 ggcggtccat gcgacagcca ggccctttcg atctcttgcc atcgccaggg caggaatagc
     1201 gaaggagctg accgacgcgc gccccggtat cggatcggaa gcaacctcgg ggctgaccgt
     1261 cggttgggcg aatgctgtaa cggtgtggag caggagaaga gaaaggagga tctttttcac
     1321 gatgtaagtt acggccttgc cacgcaaaac gtctgcaaaa cggcggctgc attgccccat
     1381 ccggtgctac actttctctc gtgcgtaagt ggtgggtggg actcctcgga gctcttcttc
     1441 ttccggcggc gctcgctgcc gcgtcttcct cgaagattct cgcggtcgcg aatgtcacgc
     1501 ccgagctgcg cgcgtcgctc acttctgact atcgtctcga catcgtcgtc aagccgcacg
     1561 tgggcgatgc ctggagccgg ctggccaaac gcgtgaccgg cgacggtgcg cgctggaacg
     1621 agatcgcatc gttcaaccag gcaggcggca acctcaccac cgagcaaagc gttcgtatcc
     1681 cgttcaacat tctccgtccg aatcttcagc acgatgtcgt ggcgatgttg tttccgtccg
     1741 acagcgcggt cgacagcggc tggcggcatg tcgtcgttgg cagcagtggc atcgaaggag
     1801 agtcgctctg gaacatcgcg gaatggttca cgggcagcgg cgagaactac gccgccatcc
     1861 gcgccgccaa tccgtcgcaa ggcctctcca cccgcaaagg tgacgtgatc ctcatcccga
     1921 aggagcttct cgcttcggca ttccgccgcg gcgaaatgga ggaacggtat gcaccgaaga
     1981 cagccgcggt tcggaaatcg gaggacgatc cggaagagcg tacgagcacg gatgctcatg
     2041 gtgcggcagc gccgctacct gaaaccgtgg ccctgggcga atccgctcag ccgtcgctga
     2101 cgccttcgct gacctacgac cggaccgccg ccgaaccgtt cgcggtctac cggctgcaga
     2161 aaggcgaagc gctctattca tcggtggcca tccgcttcac cggacgcgtc tacgcgaaag
     2221 acgtcggcga tgtgctggag cgcatcgagc gcttcaacgg catcgacgat gtggcgcgga
     2281 ttccgatcgg ctatcgcgtg aagattccga tgtcgctgct gctgcctgag tacctgccag
     2341 cggacgatcc cacgcgcgtc gccagcgaac agtccaaacg cgaaagcgag atgctggccg
     2401 tacgccctcg ggccaaaggc ctggccggtg tgcgcgtgat cctcgacgcc ggccacggcg
     2461 gacgcgacgc cggcgccacg tacgacgatg tctgggaatc gaactatgtg tacgacgtga
     2521 tgtgccgcct caagcgcatc ctcgagaaga agtccggcgc gacagtttcg gcgacgacga
     2581 agtcgaagga agccggttac gccatccccg acggcgatga gctggaggag cagaccgatc
     2641 acatcatcct gacgtcgccg aagtacgtga tcggcgatcc ggcggtgggc gtgaacctgc
     2701 gctggtacct cgccaactcg atcttccggc gcgccatgaa acagagcgtc ccgaaggaga
     2761 aggtggtctt cctttcgatt cacgcagact cgctccatcc gtcgttgcgc ggcgcgatgg
     2821 cgtacatccc cggccagcgt tacgtgaccg gctcgtacga gaagagcgac caggtgtatc
     2881 tcgcgcgtgc cgaggtgagg gagagcccgg tcgtgcggca ttcggaaaag gagtcgctgg
     2941 ctgccgaagg gctgtcgcgc gatctcgcgg aatcgatcat cgacgcgttc gagaccgacg
     3001 acctcaaagt tcacccgttc caccctgtgc gcgacaacgt cgtccgcgat ggacgcgaat
     3061 gggtgcccgc cgtgatccgc tacaacctcg tccccacccg cgtcctcctc gagatctgca
     3121 acctcggcaa cgagaaagac cgcgcgctga tcaagacgaa aaagtaccgc cagcacatcg
     3181 cggaggcgat ctacgaaggc ctggtgaact actacaacga ccgcgacgac gtccgtgggg
     3241 caccgtccat cgccagcggg cggtgaagtc cgcctccagc cgagcctctc tgtgacctct
     3301 gtgcctctgt ggtgaccggt ttttcaaatt gaccaacaac cgacacgccc ccgcgaaccc
     3361 gcgcatataa tcgcccgccg ctatgccccg ctcttttgaa ggcgatcaca acgccgaagg
     3421 tcttcgcttc gctgtgattg ccagccggtt caacgacgag atcgtcgacg gactccttcg
     3481 tggcgcgctc gaatgcctgg ccagacacgg cgccgcggac gacgacatca gcgtctaccg
     3541 cgttcccggc gcgttcgaga tcccgacgct ggcgcgcatg ctcatcgatc gcggcggcat
     3601 cgacgccgtc atcacccttg gatgccttct gcgcggcgac acgcctcact tcgatttcat
     3661 ctcctcgcag gtcacgaacg agctgtcgca cgtcgccgtc aagacgcgct ttccggtcgc
     3721 cttcggtgtc atcacgtgca acacgcgcga gcaggccgag cagcgatcat cgccgggcag
     3781 cggaaataaa ggttgggagg ccgcgttggc agcggtcgag atggcaaacc tgtggcgcga
     3841 gatcaatagc ggtgaattct gatgggcgcg cgaagaaaag cccgcgagct ggtcctgcag
     3901 atgctctacc agcacgatat gtccgggaac gcgcccgaca tgatcgtcga cacgttcgac
     3961 gatctgcaga agtcgaagcc gaacacgcgc gacttcgcca cgaagatctt ccgcggcacg
     4021 gtcgacaacc tgttcgagat cgacggcatg atccaggccc aggccgacaa ctggcgcctc
     4081 tcgcgcatgg ccgtcgtcga ccgcaacatc atccgcatgt cgatctacga gttcctacac
     4141 gaagcgggca ctccgaagct ggtcgtcatc gacgaagcca tcgagatcgc gaagaagttc
     4201 gggacggaga agtcctcgca attcatcaat ggcatcctcg acgggatact gaaacgctac
     4261 aatctcgcgt cgtgatcgaa aggcagatgg cagagggcag aaggcagaag ggcgccgcct
     4321 ctccacgttt gccttctgct ttctgctttc tgcctctcgg agaactccgc tcgtgagtct
     4381 tctcggccgc ctcgaagacc tttctctcac cgacatcgtt cagatcgtct atctcagccg
     4441 gcgcaccggc gtcctggaga tcctcgatga gcatggacgg cacacggtgc tgttccggca
     4501 cgggctggtg gtcaatgcct cgtcgcccga gcatcccgac ctgacctcct acctcgctgc
     4561 gaaaggcctg gtacccgagg cggcggtgcc gggtctgcgg caaatggagg agaaaggcat
     4621 cccgtgcggc acggctgcaa tcgagatgaa cttcctcacc gccgccgatc tcgcgaccgc
     4681 cgttcacgag cggatcgtga gcgtcgtcgc cccgctgttg cagagccggg agggagagtt
     4741 caacttcatc ctcagcgacc acgtcggccc gctcgacgtc gagtacgacg ccgacgcgat
     4801 cttcaaggaa ggcgggttcc ctccgcagaa gatcgtcggt gccgccgacg gcgagaagct
     4861 gaagccgttg cgggggctcg aggagtcgat gaaggtcggc aaagcgctgc tgcgaggctc
     4921 ggcctccccc gaagcgcccg caccttctct cgaccttggt cttggccaag caaaaccacc
     4981 ggcgctttcc gtggtcatcg aggacaacgt ccttccgttt ccaacgccgg aagagaggga
     5041 cgacgcgacg tttgcgcccg ttcccgacga cgagcctttc ccggacgtcg cgccagcgcc
     5101 ggaattgaag ccagctcccg agatcgaaga accgaaactg cggccagtac cgcccgaacc
     5161 ggcacccgca cctccggaac aggcacccct atcgcgggca tcgcagttca aagtcgcggg
     5221 tggcctgatc gaggtcgaat cgccggacgc cacctatcgc aacgtcgtcc tcttcgagcg
     5281 caacccgctc atccgcgtcg cggcgcgccg ggcattcggc agaaatggcg tgaagatcgc
     5341 gcagttcgga tcgcttgatg acgcccgcgt agccatcggc gactacatcc gcgccaatac
     5401 gttcttcgtc acgtttctcg aattgagtgc ggacgccgaa gtgctgatgc agcagctcaa
     5461 acgaaagaac ccgcggctgc cggtggtggt catcgacgcc gagccggacc tccggcggcg
     5521 gcacgatctg ttgcgcaagg gtgccgatct ctatctcacg aagccctcgg ccgcgcgcct
     5581 ccagcccggc ctggccgagg aggagctcag cctcttcgcc gatgagctgg tgctctttgc
     5641 ggagcgcgcc tttcagcaat gggagcagtt caccggcggc ctcgatcccg atgccggaaa
     5701 gcgcttctac gagacggccg aaaaggaaag cgtggaccgc agcttcggac ttctcaagca
     5761 actcatcaat gaattatcga atccgaacga catcggcgag gtgtcggcga cgatccttcg
     5821 cctctctgcc gagtatctcg accgaggcgc gatcttcatc gcaggcgatc acatgttcac
     5881 gggcgtggct ggattcggcc cgaccgatgg cggtgaggac atgaccaagc ggatcaaatc
     5941 gctgaagctt ccgcgcgccg tgccgtcgat cctgagcgat gtggcgtcct cgggtgaagc
     6001 gcaccgcggc aagatgcggc gcactcctgc caacgtggag ctgatcaact ccctcggcgg
     6061 tcttcttccc tccgaggtcg tcgcactacc gatcatgcac gcgaaacgaa cgattggtat
     6121 tctgtacggc gacaacgcag cgcaccgcgc acccatcgat gccatgacag ggctggagat
     6181 tttcctttcg caggccggat acgcattcgg aaatgccgta ttcgcagcgg agaaggcagg
     6241 ccgttgaaag tactcgtcgt cgaagattcg agctccatgc gtgcctacct cacgacgatc
     6301 atcgagggtg ggacggaagc gtacgacctg gagatcgtcg aagcagccag cggattcgaa
     6361 gcgctgaaga cgctgccgca ccacaagttc gacgccatcc tcaccgacat caacatgccc
     6421 gacatcaatg gcctggagct ggtctcgttc ctcaagaacc atccggtcca ccgctcgatc
     6481 ccgatcatgg tgatctccac cgagtcgcgc gacgaagacc gcaaacgcgc tgctgccctg
     6541 ggcgccgagg agtacctcgt gaagccgttc caggcctcgg agctgatcga aaagctgcgc
     6601 aggttactga aggtggcgta ggggcggctc ggcgtctcca tctgcgttca tctgcgtcat
     6661 ctgcggataa aaccccggtc cgccgatgac gcagatgaac gcagattaca atgcccccga
     6721 atgtccaaag aagacgatcg ggcgctgaac gagttcactg gcgaggcgga agagctgctg
     6781 gacacgctct cgcgcgatct cgtcgagctc gaggcgcagg ggcgcgacgt ccggcctgag
     6841 gtcatcaaca agatctttcg cgaggtgcac tcgctcaagg ggctggcggg catgctcggc
     6901 ttcggggaga tctccgagct ttcgcacacg ctcgaggaca tgctcgatcg cctgcggatg
     6961 ggcaagatcg agatcactcg cgacctcatc gaccttcttt acgatgccgt cgactccctg
     7021 aaccggctcg tcatcgccat caatgatcct gcggtgagcg gattgatcga catcaccggc
     7081 ctcacgcggc gcatccatca cgtcatcagc agtcagccgc agaaggcgca cgatgatccg
     7141 ttcgccgatc tcaccctcga cgagcagacg aaaaagtcgc tgacggagta cgaggagcat
     7201 cgcctcgtcg agaacgtccg cggccgcaag cacatcctcg cggtcgaggt gcgcttcgac
     7261 ttctctgatt tcgatgagaa gttgcgcgcg ctcaccgagt tgctgagcca gtcgggcgag
     7321 gtcatctcga cgttgcctgc gatcgacagc agtggcggcg ccggcatcgc gttccgcctg
     7381 ctctacggct cgacgatgga cgtcgatgag gtggccgcgc tcgttccgga aggcagcgtc
     7441 acctcgctgc gagttgcaga gttaccgggg agatcgcagc cggcggatgc agcagccccc
     7501 atcgaaatac cagaagaaga tctctccctt cgttcgatct cgcagaccgt gcgggtcgac
     7561 atcagcaaac tcgatcacgt catgaacatc gtcggggagc tgatcatcga aaagacgcag
     7621 ctcgatgcgc tcacgcgctc gctgcatcag ggggaagcgc tgcaacaggc gcggctcacg
     7681 gcgcacgagc tgaccaagat cgcaaggaac ttcgaccgca agctcaacga gttgcagaag
     7741 tcggtcatcg aaacgcgcat ggtgccagtc gggcagatct acgcgaagct ctcgcgcacc
     7801 gtgcggaagg tggcgcgcga gctgaacaaa gagatcgagc tggtgttgcg cggcgaagat
     7861 acggagctcg acaaagtgat ggtcgaggag ctgaccgacc cgctcatgca catcatccgc
     7921 aacgcgctgg atcacgggat cgagtcagcc gaggagcgcg tgaaggcggg aaagaacccg
     7981 atcggacgga tcacactcaa cgcctaccag cagggcaact cggtcgttct cgacgtcacc
     8041 gacgacggac gcgggatcga tcccgaaaat gtgcgcaagg cggctatcaa gcggaacctg
     8101 atctcgcagc acgatccctt cgatcaggtg cgcgcgtacg aaacgctctt tacgcccggc
     8161 ttctcgaccg catccgcggt cagcgaaatc tccggacgcg gcgtcggcct cgatgtcgtg
     8221 aagaagaacc tccaggagct gaagggaacg atcgacatcc gttcggttcc gggcgaagga
     8281 tcgacgttcc gcatcatgct tccgatcacg ctggcgatca ttcaggcgct ggtcgtgaag
     8341 gcgggcggcg agcagttcgc cattccgctg acgtcagtcg aggaatcgct gcgcatctac
     8401 tcgcgtgaca ttcgcacagt cgagcggcgc gaggtcttca cgctgcgcga tttcactctc
     8461 ccccttctcc gcctcgccga tgccttccac ctcggccatg agcgcgacga gaaccccgac
     8521 tcgaagtggt tcgtcgtcgt gacccgttcc ggcgagaagg tggccggcat cctcgtcgac
     8581 gcgctggtgc gtcagcagga ggtggtcatc aagtcgatcg gcgagcgttt gaagacgatc
     8641 ccgggcattg ccggggcaac cgaagtcggc gaaggagaga tcgtgctggt gatcgacgta
     8701 ggcactctga tcgagcagtt cggaggcaaa gcacgcgaga tgcgcgcggc ggtggcgtgg
     8761 gtgacgtgaa gcagtgattc gcagataact caatgttcga agaattcttc ggcttcacca
     8821 ccaagccctt cggcaagacg cccgatccgg gattcctgta cgaaagcccg cagcatagcg
     8881 aggcgctggc gcgtctggag tatgcggtcg aagagaaaga gctctcgctg ctggtgggcg
     8941 acatcggatc ggggaagacc actctttcgc gcgctctgat cgatcggatc ggcgagtcgc
     9001 gtccggtgat cctgctgatc aatccgcggc tcacgccggt gcagcttctt cgctcgatcg
     9061 catcaggcct cggcatccag cccgcgcgct accggaacga tctcctcgat cagattcacg
     9121 ccaagctgtt cgagctttac gaggcgggcc gcgagccggt attgatcatc gacgaggcgc
     9181 agctcattcc gtcgaaagcg accttcgacg agatccggct gctgacgaac tttcagctcg
     9241 atgaccagaa cctcctatcg gtgctcctca tcgggcaacc ggaactggaa gtacgccttg
     9301 agcgatccca ttacgcgccg ctccggcaac gcatcgggat gcgctatcgc ctcgggccac
     9361 tctcgctcga ggaaacgacg cgctacgtcg aacaccgcat tcgtgtggcc ggcggtacgc
     9421 gcaatccgtt ttccgaggac gcagtgaagg caatgcacgc cgtgagcggc ggcatcccgc
     9481 gcgtaatcaa cacgctcgcc acgaccgcac tgcttgatgc cttcggcgac gatgccccga
     9541 cgatcgatgc ctcacgcatc gccgccgccg cccgggaaca ccgccttcgc gaggctcctc
     9601 ctgtgaatgg agataggatc gagcaaccac atggttgatc tggtgaagct gcggaagaag
     9661 gcgaagggcg cgcgggcggc gggagacgca tcggcggaga agaggccgga tgtcgggagt
     9721 cgggagtcgg agatggttcc ggcagaagtt cctgctccaa cccccgaccg ccggcccccg
     9781 gcgttactcg aaccatccac gttgtcgcaa tccgaacgac cgacgatcaa aaagccagct
     9841 ccgacc
//
LOCUS       DATLVW010000772         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_366573, whole genome shotgun sequence.
ACCESSION   DATLVW010000772 DATLVW010000000
VERSION     DATLVW010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366573"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1517)
                     /locus_tag="VNN25_22625"
     CDS             complement(3..1517)
                     /locus_tag="VNN25_22625"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HXH94390.1"
                     /translation="MKTTSWIAAALAVGLLIPSLHAAAPAAAYKPSAAYEWLDIALEA
                     TAREVERDGARPTIISRTLAIALTAMYDAWAAYDDKAIGTRLGASLRRPPSERTQANK
                     EKAIAYATYRALIDVYPEDRSWIDEQMRGRGYDPDDISMDRTTPQGIGNVAAAAVVAY
                     RHHDGANQLGDEVGSSGVPYSDYTYYAPVNTSTKLTDPNRWQPIPFVNPKGGTIAPGF
                     LTPQWYRVKTFALDRSDQFRPPVPPHVDSEELRKEVDENIAMNNSLTPTQKAMVEFMR
                     DGPRSTGQSGHWLKFAQLVSKRDHNDLDRDVKLFFTIGNVAMDAFIACWEAKRFYDTS
                     RPWTLVRYYYAGKTIDGWAGPGKGVVKLPAEEWHPYSPFTFVTPPFPGYVSGHSTVSG
                     ACAKTLELFTGSDFFGEVEKRKAGALTEAEADCLAIQAREGKNAAAADCVVELPLPTF
                     TATAELAGISRVLGGYHIQADNIQGLALGRKVAQFEWPRIRAYFDGTASPATER"
     gene            1641..2282
                     /locus_tag="VNN25_22630"
     CDS             1641..2282
                     /locus_tag="VNN25_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXH94391.1"
                     /translation="MSKGRQTREAVLDAAVAAASTSGLSAVSIGDLARSVGMSKSGVF
                     AHFESKENLQLEVLRSAVAHFIAEVVGPALRKPRGEARVRALFENWFDWSSKASLPGG
                     CIFIAAASEFDDQPGPVRDYLVETQKDWLATLSQAVRIAVDEGQFRSDVDAQQLAHDF
                     YSILLAYHHFSRLLRMPAARVRAFRSFEQLIESARVRNAVPRDSHARKTGARK"
     gene            2303..>2630
                     /locus_tag="VNN25_22635"
     CDS             2303..>2630
                     /locus_tag="VNN25_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXH94392.1"
                     /translation="MEKNRQQKSTNVRVPPAIRIAGLALTALDVITPSLAERLAARLF
                     LTPRRVPSPASEAASRQSADRESRLVASHTITVRSWGAGPAILLLHGWEGRGTQLGAL
                     AESLARE"
BASE COUNT          477 a          833 c          828 g          492 t
ORIGIN      
        1 catcagcgtt ccgtggcagg acttgccgtg ccgtcaaagt aggcgcggat acgcggccat
       61 tcgaactggg caaccttccg cccaagtgca aggccttgga tgttatcggc ctggatgtga
      121 tagccgccca gtacgcgcga aataccggcc aactccgccg tcgccgtgaa cgtcggcagc
      181 ggcagctcga cgacgcaatc cgccgcggcg gcgttcttac cttcacgagc ctggattgcc
      241 aggcagtcgg cctccgcttc cgtcagcgcg cccgctttgc gcttctcgac ctcgccgaag
      301 aaatcgctgc cggtgaaaag ctccagcgtt ttcgcgcacg cgccgctgac ggtgctatgt
      361 cccgagacgt agccgggaaa tggcggcgtc acaaacgtga acggcgaata cggatgccac
      421 tcctcggcgg gcaacttgac gacgccctta cccggccctg cccacccgtc gatcgtcttg
      481 ccggcgtagt agtaacggac gagtgtccag ggccgcgacg tgtcgtaaaa gcgcttcgct
      541 tcccagcagg caatgaacgc atccatcgcc acattgccaa tggtgaagaa gagcttcacg
      601 tcgcgatcga gatcgttgtg atcgcgcttc gaaacgagct gcgcgaactt cagccaatgc
      661 cccgactgac cggtcgagcg cggaccgtcg cgcatgaact cgaccatcgc tttctgcgtt
      721 ggcgttaggc tgttgttcat ggcgatgttc tcgtccacct ccttccgcaa ctcctcggaa
      781 tcgacgtgcg gaggaacggg aggccggaac tgatcgctac ggtcgagcgc gaacgtcttc
      841 acgcgatacc actgcggcgt gagaaatccg ggcgcgatgg tgcccccctt cgggttgacg
      901 aacggaatgg gctgccaccg attcggatca gtgagttttg tagacgtgtt caccggcgcg
      961 tagtaggtgt agtcggaata cggcacgccg ctcgatccca cttcatcgcc gagctgattc
     1021 gcgccgtcgt gatgccggta ggcaaccacc gcggcggcgg ccacgttgcc gatgccctgc
     1081 ggcgtggtgc ggtccatcga tatgtcgtcg ggatcgtagc cacgtccgcg catctgctca
     1141 tcgatccaac ttcggtcctc gggatagaca tcgatcagcg cgcgataggt cgcgtaggcg
     1201 atcgccttct ccttgttcgc ctgcgtccgc tccgatggag ggcggcgcag gctcgcgccg
     1261 agccgcgttc cgatggcctt gtcgtcgtat gcagcccacg cgtcgtacat cgccgtcagc
     1321 gcaatcgcca gcgttcgcga gatgatggtc ggccgcgcgc cgtcacgctc gacttcgcgc
     1381 gccgtcgcct ccagcgcgat gtcgagccac tcgtacgcag cggacggctt gtaggcggcc
     1441 gccggcgccg cggcgtgcaa ggaaggaatg agaagaccta cggccagcgc cgcggcaatc
     1501 caactcgtcg ttttcatggg aactaatacg ttacgacgag tgtggccgtg aatcaagcga
     1561 actgcgtcac gattgcctgg gatcggtatc agccaacgga cggtaattgc aaaaacgaac
     1621 gatcgtgcta ttctggctgc gtgagcaaag gtcgccagac ccgtgaagcc gttctcgatg
     1681 ccgcggtcgc tgctgccagc acttcgggac tctcagcggt gagcatcggc gacctggccc
     1741 gaagtgttgg gatgtccaag agcggcgtgt ttgcccactt cgaatcaaag gaaaacctgc
     1801 agctcgaagt cctgcgatcc gcggtggcgc acttcattgc tgaagtcgta gggcccgcgt
     1861 tgaggaagcc gaggggcgag gcacgggtaa gagcgctgtt tgagaactgg ttcgactggt
     1921 ccagcaaggc ttcgctgccg ggaggctgca tcttcatcgc cgccgccagc gaattcgacg
     1981 atcagcccgg gccggtacgg gattatctcg tcgagaccca aaaagactgg ctggccacgc
     2041 tcagtcaggc ggtgcgcatc gccgtggatg aagggcagtt ccggagcgat gtcgacgcgc
     2101 agcagcttgc tcacgacttc tactcgattc tgctggcgta ccaccacttc tcgcggctgc
     2161 tgcggatgcc ggcggcgcgg gttcgggctt tccgatcgtt cgagcagctc atcgagtcgg
     2221 cgcgcgtgag gaacgcagtg cctcgcgatt cgcacgcacg caagactggc gcgcgcaagt
     2281 agaaattcag gagaagaccg gcatggagaa gaaccggcaa cagaaaagca cgaacgttcg
     2341 tgttccaccg gccattcgga tcgccggtct tgctctgact gcgctcgatg tcatcacccc
     2401 ttcgctagca gagcgcctcg ctgcccgtct cttccttact ccacggcgcg ttccctcgcc
     2461 cgcaagcgag gcagcgtccc ggcaaagcgc ggaccgtgaa tcccgtctcg tcgcttcgca
     2521 tacgatcact gtccggagct ggggagccgg accggcgatc ctcctgctcc acggctggga
     2581 aggacgcggg acgcaactcg gagcgttggc cgagtctctg gcgcgcgaag
//
LOCUS       DATLVW010000773         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_528985, whole genome shotgun sequence.
ACCESSION   DATLVW010000773 DATLVW010000000
VERSION     DATLVW010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528985"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VNN25_22640"
     CDS             complement(<1..913)
                     /locus_tag="VNN25_22640"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HXH94393.1"
                     /translation="MTITSDTPVRALPGAGTSRAKQLEEAGFATAYDLIYNLPYRYED
                     RRHPVRIVDLGGHVDKPVLLRGKVMNAVLHITRLRRMKVFEVVLEDGTGAVKLVWFNQ
                     GFLADQIKRGDRLAVFGTPKLSAYGQLQVESADWEKFEGDDEADGAIVPIYSKVGNIP
                     PKALRQIIGGALAALPSIDDPLDPALRKRLGVIDLAPALEQIHRPPQLDDAFLARRSR
                     AHIRIILQEFFTLQLALRVRRASEEVVTKTRKIVLDDVMRAEVKRILPFRLTNAQKRV
                     VREIAADLQSDKPMYRLLEGDVGSGKTI"
     gene            complement(1016..1795)
                     /locus_tag="VNN25_22645"
     CDS             complement(1016..1795)
                     /locus_tag="VNN25_22645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXH94394.1"
                     /translation="MKTVFKTTGGKEPVKDSASYAAGDVIFREGELGTEMFIIQEGEV
                     NIIKHLNGESHMLSHLEKGDFFGEMAVLESVPRTADAIAVTDVRAVVINGARFDEMLR
                     KNPEVAVRIIRKYSKRLREANTLLERLVGREVHSEHGSLDSTVSAMPEKAHRHRLIDV
                     TTGAAFPFSKADETTIGRADPVTGILPDIDLTPVDLNRSVSRRHAKIIRSGNDYCILE
                     EVGTVNGTYVNDQRIPTGTPVTIHSGDALKIGLIAMKAQFD"
     gene            complement(1792..2703)
                     /locus_tag="VNN25_22650"
     CDS             complement(1792..2703)
                     /locus_tag="VNN25_22650"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXH94395.1"
                     /translation="MLIRPRALPRNGSVVVLAMSSPSELPRIEAAARTLEQRGLRVTL
                     AANIDHRHRGYLAGDDAERLSELNHYLRSDDIDAFFFARGGYGAMRILDGVDYDAIRA
                     NPRPIVGFSDITALHQAVAVKCGIGSFHGPMLNLDWAEGLSPAIEEWFWSMLAGDAPL
                     IHHFERSQVVIDGEADGVLFGGCLSLTTALTGTPYDFWVEDGIWFFEDVDEPVYRIDR
                     MLTHLRLSGRLKKIRAVVIGKLKGCGSDAEIEALLHEFFASSNIPVIRDLPFGHQGDN
                     LLMPIGVPVRVSTRDLTLTFAQPAVQR"
BASE COUNT          499 a          809 c          896 g          546 t
ORIGIN      
        1 cgatcgtctt gccgctgccg acgtcgcctt cgagcagccg gtacatcggt ttgtcggatt
       61 gcaggtcggc cgcgatctct ctgaccaccc gcttttgcgc gttcgtcagg cggaacggca
      121 atatcctctt gacctcggcg cgcatgacat cgtcgaggac aattttccgc gtcttcgtca
      181 ccacttcttc gctggcgcgg cgcacacgaa gcgcgagctg cagcgtgaag aactcctgca
      241 ggatgatgcg gatatgggcc cgcgagcggc gagcgagaaa cgcatcgtcg agctgtggcg
      301 ggcggtggat ctgttcgagc gcaggagcga gatcgatgac gccgaggcgc ttgcgcagtg
      361 cgggatcgag cggatcgtcg atcgatggaa gcgcggctag cgcaccaccg atgatctgtc
      421 gcagggcttt cggcgggatg ttgccgacct tcgagtagat cggaacgatg gcaccgtcgg
      481 cttcgtcgtc cccttcgaac ttctcccaat cggcgctctc gacctggagc tgaccgtatg
      541 ccgagagttt cggcgtcccg aacacggcca gccggtcgcc gcgcttgatc tggtcggcga
      601 ggaagccttg attgaaccag accagcttca ccgcgccggt tccgtcctcc agcacgacct
      661 cgaatacctt catccgccgc agccgggtga tgtggaggac ggcgttcatc accttcccgc
      721 gaaggaggac cggcttgtcg acatgaccgc cgaggtcgac gatgcggacg ggatggcggc
      781 gatcttcgta gcggtatgga aggttgtaga tcagatcgta cgcagtcgcg aaccccgcct
      841 cttcgagttg tttggctcgg ctcgttcccg cacccggcag cgcgcgaacg ggcgtgtcag
      901 aggtgatcgt cacgtagcta ttgtcgcgtg cggcggggaa atcacggaag gatctctacg
      961 aggaggagat caggatgaag ttcgaaggat gaacgaatcg ctcatccttt ctcttctagt
     1021 cgaactgcgc cttcatcgcg atcagcccga tcttcagcgc gtcgccgctg tggatcgtca
     1081 ccggcgtccc cgtcgggatg cgctggtcgt tgacgtaggt gccgttgaca gtgccgactt
     1141 cctcgaggat gcagtagtcg ttgccgctgc ggatgatctt ggcgtggcgg cgcgagacgg
     1201 agcggttcaa atctacaggc gttagatcga tgtcggggag gatgccggtg accggatcgg
     1261 cgcgtccgat ggtcgtttca tctgctttgc tgaacgggaa ggcggcaccg gtcgtgacgt
     1321 cgatcagtcg atggcgatgc gctttttcgg gcatcgcgct caccgtggag tcgagcgagc
     1381 cgtgctcgct gtgcacttcg cggccgacga ggcgctccag gagcgtgttg gcctcgcgta
     1441 gccgcttcga gtatttcctg atgatgcgga cggcaacctc gggattcttg cgcagcattt
     1501 cgtcgaagcg cgcaccgttg atcacgacgg cgcggacgtc ggtcacggcg atggcgtcgg
     1561 ccgtgcgcgg aacgctctca aggaccgcca tctcgccaaa gaaatcgcct ttctcgaggt
     1621 gggagagcat gtgcgactcg ccgttgaggt gcttgatgat gttcacctca ccctcctgga
     1681 tgatgaacat ctcggtgccg agctctccct cacggaagat cacgtcacca gcggcgtacg
     1741 atgcggagtc cttcacgggt tccttcccgc cggtcgtttt gaaaacggtt ttcatcgctg
     1801 gaccgccggt tgtgcaaacg tgagcgtgag gtcccgggta ctgacgcgga cgggtacgcc
     1861 gatcggcatc aggaggttgt caccctgatg gccgaacggc agatcgcgta tgaccggaat
     1921 gttgctggag gcgaaaaatt cgtgcagcag cgcctcgatt tcggcgtcgc tcccgcaccc
     1981 tttcagcttc ccgatgacca cagctcggat ttttttcagt ctaccggaca gcctgaggtg
     2041 ggtcaacatc cgatcgatcc gatagaccgg ctcatcgacg tcttcgaaga accagattcc
     2101 atcctcgacc cagaagtcgt acggcgtgcc ggtcagcgcg gtcgtcagcg acaggcatcc
     2161 accaaacaag actccgtcgg cttcgccgtc gatgacgacc tgcgagcgct cgaaatgatg
     2221 gatgagcggc gcatcacctg cgagcatcga ccagaaccat tcctcgatcg ccggtgacaa
     2281 cccctcggcc caatcgagat tgagcatcgg tccgtgaaac gagccgatgc cgcacttgac
     2341 ggcgacggcc tggtgcaacg cggtgatatc gctgaagccg acgatcggcc ggggattggc
     2401 gcggatggcg tcgtagtcga cgccgtcgag gatgcgcatg gcgccgtagc cgccgcgggc
     2461 gaagaagaag gcatcgatat cgtcagacct caagtaatgg ttcaactctg agagacgctc
     2521 ggcatcgtcg ccggcgaggt agcctcgatg gcgatgatcg atgttcgcag ccagcgtgac
     2581 gcgcaaccct cgctgctcga gcgttcgcgc cgccgcttcg atgcgcggca gttcggatgg
     2641 agatgacatg gccaggacga caacgctgcc gtttcgtggc agcgcacgtg ggcgtatgag
     2701 cacgaacgga gagtagcaag ggcggtgcta gagcgtttgt gagagaagag
//
LOCUS       DATLVW010000774        15731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_158334, whole genome shotgun sequence.
ACCESSION   DATLVW010000774 DATLVW010000000
VERSION     DATLVW010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15731
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158334"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VNN25_22655"
     CDS             <1..594
                     /locus_tag="VNN25_22655"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXH94396.1"
                     /translation="VAIVLLDYTLWWWHWASHRVPFFWRFHLVHHIDRDLDSSTALRF
                     HVGELALSIPVRAAQMVIIGVDPQALWLWQTILFGSILFHHANVRLPLRVERVLVRLI
                     VTPRMHGIHHSEVLNETNSNWSSLLSCWDYLHRTILLNVPQPEITIGVPAYSEASDVT
                     IGKMLVLPFKRQRSDFHLTDGTLAIREHEKGNRSLVP"
     gene            complement(619..1644)
                     /locus_tag="VNN25_22660"
     CDS             complement(619..1644)
                     /locus_tag="VNN25_22660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXH94397.1"
                     /translation="MTPDHHEHPHDGTVHGHEAHPHPHDHEPHGAGGHGHTHGVVDSS
                     ILTSERGIWAIRWSFLVLFFTALIQIYVYYRSSSVGLLADTIHNFSDAATAVPLWIAF
                     TLSRRRPSHRFPYGLGRVEDLAGVTIVFVILFSAVAAGYQSIQRFFHPVLLSHLYAVM
                     VASVVGFAGNEIAAIIRIRVGKEIESAALIADGNHARVDGIASLAVLFGALGVKLGYP
                     LADPIVGILITLIICKVVWDAVAAVFTRMLDGVDPALIPQLTDITRKTEGVADVGEVR
                     ARWLGHRLMAEVTFGVPPSLTVAHGHEVAKLVQANIQKALPHVAAVAVHVDPSDELGE
                     EHHHRRL"
     gene            1916..5590
                     /gene="metH"
                     /locus_tag="VNN25_22665"
     CDS             1916..5590
                     /gene="metH"
                     /locus_tag="VNN25_22665"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXH94398.1"
                     /translation="MAQPDRTAELRELLDRRILVLDGAMGTMIQRYRLDEAAFRGVQF
                     AAHPRDLRGANDILCLTRPDVVESIHRQYLEAGADIIETNTFNAQAISLADYDLVEYS
                     YDINKAAAELARKACDAYATPERPRFVAGSLGPTNRTASLSPDVNNPAFRGVTFDELV
                     DAYYEQTRGLVDGGADILMPETTFDTLNIKAALFAIEKYFADTGIRLPIIASITITDA
                     SGRTLSGQTVEACWNSISHANLLAAGINCALGAAEMRPHIEELAAIAPVYISCYPNAG
                     LPNALGEYDQTPEEMAALLHEYAGNNWINIVGGCCGTTPDFIRAIVDAVKMFPPRVPP
                     QVEKHLRLSGLEALTVRNDGGEGSQFIMVGERTNVTGSPKFAQIVKAGDLEPALAIAK
                     QQIDGGANILDVNMDEGLLDSEKVMTEFLHLVASEPDIARVPIMIDSSKWTVLEAGLK
                     CTQGKSVVNSISLKEGEDKFREQARIIRRFGAAVIVMAFDEQGQADSIERKVAICSRA
                     YQILVNEVGFDPTDIIFDPNVLTVATGIEEHNNYGVGFIEAVRQLHELFPLASFSGGI
                     SNVSFSFRGNKAVREAMHAAFLYHAIKAGLTMAIVNAGQLAIYEEIPPELLALVEDVL
                     LNRRPDATERLLAYADNVKQGPATAKEEEAWRSGSVEERLSHSLVKGIIEHLDEDLGE
                     ARQTYPSALSIIEGPLMDGMNVVGNLFGAGKMFLPQVVKSARVMKKAVAYLLPFMEAE
                     KAKAGDLSQRGKVLMATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVSADKILDTAI
                     AEKVDIIGLSGLITPSLDEMVHVAREMERRNFVLPMLIGGATTSKLHTAVKIAPRYSH
                     ATVHVLDASRAVGVVSTLLSDRRDEFVRERHTEYEHLREAQAKRQSEVKILSIEAARR
                     RKPALSFSPDRPPFLGVRVLDDVPLERIEPFIDWNPFFTAWELRGRFPQILDEPRAKE
                     LYDDGRKLLREIIDNRLIHARAAYGFWPANSVGDDIEVYGDESRSDVIARFHTLRQQQ
                     ETNTGRNYALADFIAPKESSIIDYIGGFAVTAGIGVAEVVERFEKDHDDYNAIMTKAL
                     ADRLAEGLAEMLHKETRAEWYAPNEDLSSEDLIAEKYRGIRPAPGYPASPDHTEKATL
                     FALLDAGRAGIDLTENFAMTPAASVSGLYFAHPESKYFSVGRIGRDQVADYHARKDMP
                     LPLVERWLAPSLAYEPETVV"
     gene            5630..6064
                     /locus_tag="VNN25_22670"
     CDS             5630..6064
                     /locus_tag="VNN25_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH94399.1"
                     /translation="MNTTQSQPNVKQAVPFFRVSDMDASLRFYLDGLGFVMTKKWIDE
                     GKLRWCWLEIGDAALMLQEFRKEGLNSWVPDAKVGVGVSICFICRDALAIYRDAIARG
                     LEASRPFVGNGMWCTSVSDPDGYRLEFESLTDVPEETVLSGD"
     gene            6191..6607
                     /locus_tag="VNN25_22675"
     CDS             6191..6607
                     /locus_tag="VNN25_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXH94400.1"
                     /translation="MRFMVLVKANKDSEAGVLPDEKILTAMGKFNEELVKAGVMLAGE
                     GLQASSKGARVRFSGGKKTVIDGPFAETKELVVGFWLWQVKSKEEAIEWLKRAPFEDT
                     EVELRQVFEMEDFGANLTPELREQEERTRAQAAARK"
     gene            6657..7913
                     /locus_tag="VNN25_22680"
     CDS             6657..7913
                     /locus_tag="VNN25_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXH94401.1"
                     /translation="MTAADIHRTIDAVWRIESARLIAGLTRIVRDVGLAEDLAQDALV
                     AALEKWPEAGVPDNPGAWLMATAKNRAIDTLRKNALHERKHEAIGRDVETVEPDLVTA
                     LDDEIGDDLLRLVFIACHPILSTEARVALTLRLLGGLTTDEIARAFLVPEPTIAQRIV
                     RAKRTLTEARAPFEVPRGKEFVARLASVLQVIYLIFNEGYAATAGDDWMRPQLCEEAL
                     RLGRILAELVPAEPEVHGLVALMEIQASRIGARVSPSGEPILLLDQDRGRWDHVLIGR
                     GLAALARGEKLGGAPGPYALQAAIAACHARAHAAEETDWRRIASLYEALAELTPSPVI
                     ELNRAVAVGMAFGPAAGLALVDELRAEPSLASYHLLPSVRGDLLFKVQRFDEAREEFK
                     RAASLTRNVRERALLLERVAACERES"
     gene            8037..9704
                     /locus_tag="VNN25_22685"
     CDS             8037..9704
                     /locus_tag="VNN25_22685"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94402.1"
                     /translation="MLWPLRIALAKLRPFNAATIIRTSIVSVLFAAFLYGDFALFRRL
                     FATMAKVEQESPFLALGLLRNLLSLVFLTAVVMLFSSSMTAAIGAFFSDLDLDTFHAA
                     PLSKTRIVVLRWGKTFAQSATMVYVFIAPMFVAFALRYNTPARLYPIVLVNLALLLAI
                     PVTLGGLMIIVLVRFFPVQRVSQIVATIAILVLTVAVVAFRMSRPERLFASINTDDVT
                     RVLRSVELPSLDRYPGTALADVMVALAEGRPASILPPKIGVTALALFAIFVFVARRIY
                     FTAFVRARESMAPAALGAGGMTRAADILLAPFSAPTRAMIGKEVRTLTRDVAQWSQLF
                     LMIALMFLYLYNIRMLPLSGDARATIVAYANLGMAGFVVAAICLRFAYPSISSEGKAF
                     WMLQSAPVSYRRLLIVKVIVYAAPLTILSLLLTTFADVLLDANRVVWTFTLFGASMLG
                     VTLVSMGVGLGSLSPDFGAENPLQVGLSLGGFAYMAAALAYVGSIMTLMAKPVMRYFF
                     WRLFGIESDGMAVDILPVVTAVTLSIALCVFPLLAAEKRLTALAESR"
     gene            9744..10169
                     /locus_tag="VNN25_22690"
     CDS             9744..10169
                     /locus_tag="VNN25_22690"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXH94403.1"
                     /translation="MSETPSSYTPPPPPPGGSYTPPPPPTPGAAAPGGDRTIMIVLSY
                     LWILALIPLLTKKEDPEIQWHAKNGLALLGAEIICWIVFMILGIILSKIFAALSCGVT
                     LIQCAVWIGFLVVRVMCIVKGTSGQRFRVPVVTDMAEKM"
     gene            10186..10557
                     /locus_tag="VNN25_22695"
     CDS             10186..10557
                     /locus_tag="VNN25_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94404.1"
                     /translation="MDTPGGSGSYTPPPPPPPPPSSGSTPPAGPSAADIVYPTDPPKD
                     PVLIAVLNLVVAGCLGYFMIGQKMKGIVAVIAWVLGLFFTCGTVSGLVSIVAAIDGYM
                     QAQQLKAGFPVAQWTFFDKHQ"
     gene            complement(10485..10901)
                     /locus_tag="VNN25_22700"
     CDS             complement(10485..10901)
                     /locus_tag="VNN25_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94405.1"
                     /translation="MENLDFAAEKLDFAVEKLDIAALNLDFDVEKLDIGVRDLDIAAE
                     NLDIAALNLDFDVENLDIGVRDLDIDVDDRDFDVSNLVIDVSDLDISATNLPCKMKKR
                     RGCGAFSRFWKLVYWCLSKNVHCATGNPALSCCACM"
     gene            11557..12804
                     /gene="hflX"
                     /locus_tag="VNN25_22705"
     CDS             11557..12804
                     /gene="hflX"
                     /locus_tag="VNN25_22705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXH94406.1"
                     /translation="MPENVIAVALILPDLTRSVAEEHLAELEKLIETAGGTVVDRVFA
                     KRPRPDPATYVGSGKAEQIKALVQRSGATLIVFDDDLSPAQVRNLEKITEVKVIDRSG
                     LILDIFAGRARSREARTQVELAQLEYMLPRLTRAWTHLERQAGGIGTRGVGETQLELD
                     RRIIRNRIARLKEELEKIERTRETQRKSRNAVFSVALVGYTNAGKSTLFNRLTSGSAD
                     AADKLFATLDAKSARAAFDMPRETVVIDTVGFIRKLPHHLVASFRSTMEEAVAADLVL
                     HVIDSSHPQFEDQRAVGEEVLRELGVVMDRVIEVYNKADRTDMFELRRKNAASVSALT
                     GRNIERLIDIIRERELENGEVIQLEIPHNDARVIAKLHDVAEVYEQRVTDRAMMITAW
                     VPRDLLHLFEAYAAVRLQPAKVS"
     gene            12975..13469
                     /locus_tag="VNN25_22710"
     CDS             12975..13469
                     /locus_tag="VNN25_22710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXH94407.1"
                     /translation="MKIRPAELRDRGRILEILIATARFTDVEVRDAMELVDKANDDPN
                     QSEYIVHVLEEPATGPRRMVQGYVCYGPTPHTEGVFDLYWIAVDPKQQGQGIGQLLLR
                     FVENEVRRQRGRMLLIETSSKESYAPTVRFYERSGYEEISRIKDFYRLADDKVVYCKK
                     LTPS"
     gene            13471..14262
                     /gene="ccsA"
                     /locus_tag="VNN25_22715"
     CDS             13471..14262
                     /gene="ccsA"
                     /locus_tag="VNN25_22715"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXH94408.1"
                     /translation="MSPNFTLYAALALYAAGTVMALVTLFTREKRPQHIALGIMIAGF
                     VAHTVWISTICIRTHHPPLTNLPEAAGFMAWVVFAIELALFIRYRVHAAAFFVYPLVL
                     MLLTVSAVVHEPFVRSATAVSRLFISHILLTTIGVAGLLVGLAFGALAWAQDRALKSK
                     QRGALWDWIPSLSVCKTLSYRALAIGFSVYTLGILAGILWSYKTSAELMDVRVKQVAA
                     MAAWVLFGVVLQAYVNDTYRTRRTAVIGACAFIAIVVAILGIHHV"
     gene            14389..15648
                     /gene="hemA"
                     /locus_tag="VNN25_22720"
                     /pseudo
     CDS             14389..15648
                     /gene="hemA"
                     /locus_tag="VNN25_22720"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
     gene            15645..>15731
                     /locus_tag="VNN25_22725"
     CDS             15645..>15731
                     /locus_tag="VNN25_22725"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94409.1"
                     /translation="MTRLVRIGTRGSQLALWQANEVANKLSEQ"
BASE COUNT         2937 a         4943 c         4838 g         3013 t
ORIGIN      
        1 gtcgcgatcg ttctgctcga ctacacgctc tggtggtggc attgggcgag tcatcgcgtt
       61 ccgttcttct ggcggttcca cctcgtgcat cacattgatc gcgatctcga ttcctccacc
      121 gcgctgcgct ttcacgtcgg cgagctggcg ctttcgattc cggtgcgtgc cgcgcagatg
      181 gtgatcatcg gcgttgatcc gcaggcgttg tggttatggc agacgatcct gttcggctcg
      241 atcctcttcc accacgcaaa cgtgcgcttg ccgcttcgcg tcgagcgggt gctggtgcgg
      301 ttgatcgtca caccgcggat gcacggcatt catcactccg aggtgctgaa cgagaccaac
      361 agcaactggt cgagtctgct ctcatgttgg gattacctgc atcgaacgat tctgctcaat
      421 gtgccgcaac cggagatcac gatcggcgtc cccgcgtact ctgaggcgtc cgacgtgacg
      481 atcgggaaaa tgctcgtgct accgttcaag cgtcagcgca gcgattttca cttgaccgat
      541 gggacgctcg cgattcgaga gcacgagaag ggaaatagga gcctggttcc gtagccgtgg
      601 atcgcgccgc attggcgctc aaaggcgccg atggtggtgc tcctctccca gctcgtccga
      661 cggatcgaca tgaacggcaa ccgcagcaac atgcggtaga gctttctgga tgttcgcctg
      721 caccagtttt gcgacctcgt gtccgtgagc gacggtgagc gacggcggca caccgaaggt
      781 cacctccgcc atcagccggt gaccgagcca tcgcgcccga acctcaccga catctgcgac
      841 accttccgtc ttacgagtga tgtcggtgag ttgggggatg agcgcggggt cgacgccgtc
      901 caacatccgg gtaaacacgg cggcgacggc atcccacacg actttgcaga tgatgagtgt
      961 gatgaggatg ccgacgatcg ggtcggcgag ggggtaacca agcttcacgc cgagcgcacc
     1021 gaagaggact gcaaggctgg cgataccgtc gacccgagcg tggtttccgt cagcgatgag
     1081 ggccgcgctc tcgatttcct tcccaacgcg gatgcggatg atcgcggcga tttcgtttcc
     1141 ggcgaagccc accaccgaag ccaccatcac cgcatacagg tgactgagca gcaccggatg
     1201 gaagaatcgc tggatcgact gatagcccgc tgccacggcg ctgaagagga tgacgaaaac
     1261 gatcgtgacc cctgcgagat cctcgacgcg acccaggcca tacggaaacc gatgggatgg
     1321 tcggcgccgc gaaagcgtga aggcgatcca gagcggtact gctgttgcgg cgtcactgaa
     1381 attgtggatc gtgtctgcaa gaaggcccac gctgctggat cggtagtaaa cgtagatctg
     1441 aatcagagcg gtgaagaaga gcacaaggaa ggaccagcgg attgcccata tgccccgttc
     1501 cgacgtcagg atcgacgaat cgacgacgcc gtgcgtatga ccgtgccctc ctgctccatg
     1561 tggctcgtga tcgtgaggat gagggtgtgc ttcgtggccg tgcaccgtgc cgtcgtgagg
     1621 atgttcgtga tgatctgggg tcatgagttt cttgcctgaa tcatctgcga ggagaagata
     1681 ggcggttgct cgagcgatgt cgaacgtcga ggggagtgat ctcggcaata tcaagcgtag
     1741 caggcatcgt ttgacaaccc tccgccgcgt gccgtaccct cgaccctcct ggactgattt
     1801 gcggggctgc ttgcaaccag gttaaaaaaa gctaaagagc cggcagtgat ccgcgttctg
     1861 acgccgattt tcagccccgc aatttccgcg gggctttttg ttttcgggag aatcgatggc
     1921 gcaaccggac cgtactgctg aacttcgtga gctgctcgat cgccgcatcc tcgtgctgga
     1981 tggcgcgatg gggaccatga tccagcgcta ccggctcgac gaagcggcct tccgcggtgt
     2041 gcagtttgct gcacatccgc gcgacctccg aggagcgaat gacattctct gcctgacgcg
     2101 ccccgacgtc gtcgaatcga tccaccgcca atacctcgag gccggcgccg acatcatcga
     2161 gaccaacacg ttcaacgcgc aggcgatctc cctcgcagac tacgacctgg tcgagtactc
     2221 ctacgacatc aacaaggctg ccgcggagct ggcgcgcaag gcgtgcgacg cttacgccac
     2281 tcccgagcgt ccccgcttcg ttgccggctc cctcggcccc acgaaccgca cggcctcgct
     2341 ttcgcccgat gtgaacaatc ctgcatttcg cggggtgacg ttcgatgagc tggtcgacgc
     2401 ctattacgag cagacgcgcg gtctcgtcga cggcggcgcc gacatcctga tgccggagac
     2461 gaccttcgac acgctcaaca tcaaggcagc gctcttcgcg atcgagaagt acttcgccga
     2521 caccgggatc cgactgccga tcatcgcttc gatcacgatc accgacgctt cgggccgcac
     2581 gttgtccggc cagacggtcg aagcgtgctg gaactcgatc tcgcacgcca accttctcgc
     2641 cgccggcatc aactgcgcgc tgggcgctgc cgagatgcgg ccacacatcg aagagctggc
     2701 cgccatcgcg ccggtttaca tcagttgtta tccgaacgcc ggactgccga atgcgctcgg
     2761 tgaatacgat cagacccccg aggagatggc ggcgctgctg cacgaatacg caggcaacaa
     2821 ctggatcaac atcgtcggcg gctgctgcgg aacaacgccc gacttcatcc gggccatcgt
     2881 cgatgcggtg aagatgttcc cgccgcgcgt gccgccacag gtcgagaagc atctacgcct
     2941 cagcggactc gaagcgctca cggtccgcaa cgacggcggc gaaggatcgc agttcatcat
     3001 ggtcggcgag cgcacgaacg tcaccggctc accgaagttc gcgcagatcg tgaaggctgg
     3061 cgatctggag ccggcactgg cgatcgccaa acagcagatc gacggcggcg ccaacattct
     3121 tgacgtcaac atggacgaag ggctgctcga ctccgagaag gtgatgaccg agtttctgca
     3181 cctcgtcgcc tcggagcccg acatcgcccg cgtcccgatc atgatcgact cgtcgaagtg
     3241 gacggtgctc gaagccggcc tcaaatgtac gcagggcaag tcggtcgtca actccatctc
     3301 gttgaaagag ggagaagaca agttccgcga gcaggcccgc atcatccgcc gattcggcgc
     3361 ggctgtgatc gtgatggctt tcgacgagca gggtcaggcc gactcgatcg agcgcaaagt
     3421 agccatctgc tcgcgcgcct accagatcct cgtcaacgag gtgggcttcg atcccaccga
     3481 catcatcttc gatcccaacg tcctcacggt cgccaccgga atcgaggagc acaacaacta
     3541 cggcgtcggc ttcatcgagg cggtccggca actgcatgaa ctgtttccgc tcgccagttt
     3601 ctcgggcggc atcagtaacg tctcgttctc gttccgcggc aacaaggccg tgcgcgaggc
     3661 gatgcacgca gcgtttctct atcacgccat caaagcgggc ttgacgatgg ccattgtcaa
     3721 cgccggacag ctcgccattt acgaagagat tcctcccgag ctgcttgcgc tggtcgaaga
     3781 tgttctcctc aaccgacgtc cggatgcgac cgaacgtttg ctcgcgtatg cggacaacgt
     3841 gaaacaagga ccggcaacgg caaaagagga agaggcctgg cgcagcggca gcgtcgaaga
     3901 gcggctctcc cactcgctcg tcaagggaat catcgagcat ctcgacgaag acctgggaga
     3961 agcgcggcaa acctatccca gcgcgctctc gatcatcgaa gggccgctga tggacggcat
     4021 gaacgtcgtc ggcaatctct tcggagcggg caagatgttt ctcccgcagg tcgtgaagag
     4081 cgcgcgcgtg atgaagaagg cggtggccta tttgctgccg ttcatggaag cggagaaggc
     4141 gaaggcaggc gatctctcgc agcgcggcaa ggtgctgatg gcgacggtga agggcgacgt
     4201 tcacgacatc ggcaagaaca tcgtcggcgt cgttctcgga tgcaacaact acgaagtcat
     4261 cgacctcggc gtcatggttt ccgccgacaa gatcctcgac accgcgatcg ccgagaaggt
     4321 cgacatcatc ggcctctccg gcctgatcac gccgtcgctc gacgagatgg ttcatgtggc
     4381 gcgcgagatg gagcggcgca atttcgtgct gccgatgctg atcggcggcg cgacgacgtc
     4441 gaagctgcat accgcagtga agatcgcccc gcgctactcg cacgcgaccg tccacgtgct
     4501 cgacgcctcg cgcgccgtcg gcgtcgtcag cacgctcctg tccgatcgcc gcgacgagtt
     4561 cgttcgcgag cgtcataccg aatacgagca cctccgcgaa gcccaggcca agcgtcagag
     4621 cgaggtcaag atcctctcga tcgaggcggc acgccgccgc aagccggcgc tttccttctc
     4681 acccgatcgt ccgcccttcc tcggcgttcg cgtgctcgac gacgttccgc tagagcgcat
     4741 cgagccgttc atcgactgga atccgttctt caccgcatgg gagctgcgcg ggcgttttcc
     4801 gcaaatcctc gacgagccgc gggcgaaaga gctttacgac gacggccgca aactgctccg
     4861 cgagatcatc gacaaccgtc tgattcacgc ccgcgccgct tacggtttct ggcccgccaa
     4921 ttcggttggc gacgatatcg aggtgtacgg cgacgagtcg cgcagcgatg tcatcgcgcg
     4981 tttccacacg ctgcggcagc agcaggagac gaataccggc cgcaactacg cgctggccga
     5041 tttcatcgct ccgaaagaga gcagcatcat cgactacatc ggcggcttcg cggtcacggc
     5101 cgggattgga gtggccgagg tcgtggaacg cttcgaaaaa gaccacgacg actacaacgc
     5161 catcatgacc aaggcgctcg cggatcgcct ggccgagggg ctcgccgaga tgctgcacaa
     5221 agagacgcgc gcggagtggt acgcgccgaa cgaagacctg tcgagcgagg atctgatcgc
     5281 cgagaagtac cgcggcattc gccccgcgcc gggatatcca gcctcaccag accacacgga
     5341 gaaagcgacg ctcttcgcgc tgctcgatgc cgggcgcgcc ggtatcgacc tcaccgaaaa
     5401 ctttgccatg acgccggccg cctccgtcag cggcctctac ttcgcacacc cggagtcgaa
     5461 gtacttctcg gtcggccgga tcggccgcga tcaggttgcc gactaccacg cacgcaaaga
     5521 catgccgctc ccgctggtcg aacgctggct cgccccgtcg ctggcgtacg agccggagac
     5581 ggtggtgtga cgacggaacg gtaaaactga tctccgcgga gatttggcga tgaacacaac
     5641 ccaatcccag cccaacgtta aacaggcggt tcccttcttc cgcgtatcgg acatggatgc
     5701 gtctcttcgt ttctatctcg acggcctcgg cttcgtgatg acgaagaagt ggatcgacga
     5761 agggaagctg cggtggtgct ggctcgagat cggcgacgcc gcgctgatgc ttcaggagtt
     5821 tcgcaaggaa gggctcaatt cgtgggtgcc ggacgcgaag gtcggcgtcg gcgtttcgat
     5881 ctgctttatc tgcagggacg cacttgcgat ttatcgcgac gcgatcgctc gcggtctcga
     5941 ggcgtcacgt cccttcgtcg gcaacgggat gtggtgtacg tctgtatccg atccggatgg
     6001 ctaccggctc gagttcgaga gcctgacgga cgtgccggaa gagacggtct tgtcaggcga
     6061 ttagccgggc acaacattcg ctccgaaaaa agtttgctcc gcgtgtcgat ttcgccgatc
     6121 gccgctcgtc gtgtagatag aggcagggaa atcctgctcc gaacgacaaa tcaatcgaag
     6181 gagaaaaata atgcgattca tggtgctggt caaagcgaac aaggactcgg aagcgggcgt
     6241 gctccccgac gagaagattc tcacggcgat gggcaagttc aacgaagagc tggtgaaagc
     6301 cggcgtgatg ctcgccggcg aaggattgca ggcgagctcg aaaggcgcgc gggtgcggtt
     6361 ctcgggcggg aagaagacgg tcatcgacgg acccttcgcc gagacgaaag agctcgtcgt
     6421 cggcttctgg ctgtggcagg tgaagtcgaa ggaagaggcg atcgagtggc tgaagcgcgc
     6481 gccgtttgag gataccgagg tcgagctccg tcaggtattc gagatggaag acttcggcgc
     6541 caatctcacg ccggaactgc gtgaacagga agagcgcact cgcgcccagg ccgccgcgag
     6601 gaagtaggcc gcggcgccgt tgcgccggcg tggtgaagat ggtgtgatcg gcagccatga
     6661 cagctgccga catccatcgc acgatcgatg cagtctggcg gatcgagtcc gccaggctca
     6721 tcgccggctt gacgcgaatc gttcgcgatg tcgggttggc cgaggatctc gcgcaggacg
     6781 cgctggttgc ggcgctcgag aagtggcccg aggccggcgt ccccgacaat ccgggcgcgt
     6841 ggctcatggc gactgcgaag aatcgcgcca tcgataccct gcgcaagaac gcattgcacg
     6901 agcgcaagca tgaagcgatc ggccgcgatg tcgagacggt ggagcccgat ctcgtgacgg
     6961 cgcttgacga cgagatcggc gacgatctcc tccggctcgt gttcatcgcc tgccatccga
     7021 ttctgtcgac cgaggcgcgt gttgcgctga cgcttcgcct cctcggcgga ctgacgacgg
     7081 acgagattgc ccgcgcgttt ctcgttccgg agccgacgat cgcgcagcgc atcgtgcggg
     7141 ccaaacggac gttgactgag gcgcgcgcac cgttcgaagt tccgcgcggc aaggagttcg
     7201 tggcgcgact cgcgtcggtt ctgcaggtga tctatctgat cttcaacgaa ggctacgccg
     7261 ccacggcggg cgacgattgg atgcgcccgc aactctgcga ggaagcgcta cgcctgggca
     7321 ggattctggc cgagctggtg ccagccgagc cggaagtgca cggcctcgtg gcgctgatgg
     7381 agatccaggc gtcgcgaatc ggtgcgcgcg tgagcccctc gggcgagccg atcctgctgc
     7441 tcgatcagga ccgtggccgc tgggatcacg tcctcattgg acgcggcctc gcggccctcg
     7501 cgcgtggcga gaaactgggc ggtgcgccgg ggccgtacgc actgcaagct gcgattgccg
     7561 cgtgtcacgc gcgagcgcat gccgctgaag agacggactg gcggcggatc gcgtcgctct
     7621 acgaagcgct ggccgagctc acgccgtcac cggtcatcga attgaatcgc gcagtagcag
     7681 tggggatggc gttcggtccg gccgcgggac tggcgctggt cgatgagctc cgtgcggagc
     7741 cttcgctggc gtcgtatcac ctgctgccga gcgtgcgcgg cgatctgctg ttcaaggtgc
     7801 agcgcttcga tgaggcgcgc gaagagttta agcgcgcggc ctcgctcacg cggaatgtcc
     7861 gcgaacgtgc gctgctgctc gagcgcgtgg ctgcgtgcga acgcgagtcg tgacgggggc
     7921 caccaaaacc tttgcaccac agagacacag aggacacaga gttgaagccg ttctctgtgt
     7981 cctcagtgtc tctgtggtgc aatctttttc gacgaaaagc gtaacctccc caccgaatgc
     8041 tctggccgct acgaatcgcg ctcgccaaac tccggccgtt caacgcggcg accatcattc
     8101 gtacctcgat cgtgtcggtg ctcttcgcgg cgtttctgta tggcgacttc gcgctcttcc
     8161 ggcggctctt tgccacgatg gcgaaggtcg agcaggagtc gcctttcctt gcgctcggac
     8221 tgctgcgcaa tctcctgtcg ctcgtatttc tcactgccgt cgtcatgctg ttttcgtcgt
     8281 cgatgacggc ggcgatcggc gcgttcttca gcgatctcga tctcgacaca ttccacgccg
     8341 cgccgttgtc gaaaacgcgc atcgtcgtgt tgcgatgggg gaagaccttc gcgcagagcg
     8401 cgacgatggt ctacgtcttc atcgcgccga tgttcgtcgc cttcgcgctg cgctacaaca
     8461 cgccggcacg gctctacccg atcgtcctcg tgaatctggc gctgctgctg gccattcccg
     8521 tgacgctcgg cggactgatg atcatcgtcc tcgtgcgctt ctttccggtc cagcgcgtca
     8581 gccagatcgt cgccacgatt gccatccttg tcctcaccgt tgccgtcgtc gccttccgta
     8641 tgagccgtcc ggagcgtctg ttcgccagca tcaacacgga cgacgtcacg cgcgtgcttc
     8701 gctcggtcga gttgccctcg ctcgaccgct atccgggaac ggccctcgcc gacgtcatgg
     8761 tggcgctggc ggaagggcgg cccgcatcga tcctgccacc gaagatcggc gtaaccgcgc
     8821 tcgcactctt cgcgatcttc gtattcgtcg cgcggcggat ctacttcacc gcgttcgtgc
     8881 gcgcgcggga gagcatggct cccgcggcgc tcggcgcggg cgggatgacg cgtgcagccg
     8941 acatccttct cgcgccgttc tcggcaccga cgcgcgccat gatcgggaag gaagtgcgca
     9001 cgctcacgcg cgacgtcgcg cagtggtcgc aactctttct gatgatcgct ctgatgtttc
     9061 tctacctcta caacatccgc atgctgccgc tcagcggcga cgcccgcgcc accatcgtcg
     9121 cctacgccaa cctcggcatg gccggcttcg tcgtggcggc gatctgtctc cgcttcgcgt
     9181 atccctcgat ctcgtcggaa gggaaagcat tctggatgct gcagagcgcg ccggtgtcgt
     9241 atcgccggct gctcatcgtg aaggtaatcg tctacgccgc gccgctgacg atcctctcgc
     9301 tgctcctcac cacctttgcc gacgtcctgc tcgatgccaa tcgcgtggtc tggacattca
     9361 cgttgttcgg agcgtcgatg ctcggtgtga cactggtcag catgggagtc ggtctcggct
     9421 cgctctcgcc cgacttcggc gcggagaatc cgctgcaggt aggtctttcg ctgggcggtt
     9481 tcgcctacat ggcggcggcg ctggcctacg tcggcagcat catgacgctg atggccaaac
     9541 cggtcatgcg ctacttcttc tggcgccttt ttggcatcga gagcgacgga atggcggtcg
     9601 atatccttcc ggtcgtgact gcggtcacac tctcgatcgc actatgcgtg tttcctctgc
     9661 tggccgcgga aaaacgttta accgcgctcg cggaaagccg ttagaatcgc ccgaaccgac
     9721 caactttcac tgaaggagaa gcaatgagcg agacaccgtc ttcgtacacg ccgccaccgc
     9781 caccgccggg tggaagctac acaccgccgc cgcctccgac ccccggtgct gccgcgccag
     9841 gcggagacag gaccatcatg atcgtcctgt cctacctctg gatccttgcg ctgattcctc
     9901 tgctgacgaa gaaagaagac cctgagattc agtggcacgc caagaatggt ctggcactcc
     9961 tcggcgccga gatcatctgc tggatcgtct tcatgatcct cggcatcatc ctgagcaaga
    10021 tatttgcagc actctcctgt ggagtgaccc tcattcagtg cgccgtgtgg atcggcttcc
    10081 tcgtcgtccg tgtgatgtgt atcgtcaaag gcaccagcgg gcagcgattc cgcgttccgg
    10141 tcgttaccga catggccgag aagatgtagt caaaaggaga ttccgatgga tacacctggc
    10201 ggttccggtt cctacactcc accaccacct cctccacctc cgccttcgag cggaagcacg
    10261 ccaccggcag gtccgagcgc tgcggacatt gtctatccga cggatccacc gaaggatccg
    10321 gtgctcatcg ccgtgctgaa tctcgtcgtg gccggctgtc tcggctactt catgatcgga
    10381 cagaagatga aggggatcgt agcggtcatc gcctgggtcc tcggcctctt cttcacctgc
    10441 ggcacggtgt ccggactcgt ctccatcgtg gccgcgatcg acggctacat gcaggcgcag
    10501 cagctcaaag ccgggttccc ggtcgcgcag tggacgttct tcgacaagca ccagtaaacc
    10561 agcttccaga atcgggaaaa ggcgccgcaa ccgcggcgct ttttcatttt gcagggtaga
    10621 ttggtcgcac tgatgtcgag gtccgagaca tcgatgacaa gatttgagac atcaaagtct
    10681 cgatcgtcga catcgatgtc cagatctcta acaccgatgt ccagattttc gacatcgaag
    10741 tccagattca acgctgcgat gtccagattt tctgctgcga tgtccagatc tctaacaccg
    10801 atgtccagct tttccacatc gaagtctaga tttaacgctg cgatgtccag cttttccact
    10861 gcgaagtcca gcttttccgc tgcgaagtcc agattttcca ctgcgaagtc cagattttcc
    10921 gctgtgaagt ccagatttcg atgtctcggt tcttcacaac gatgtggaga tcgacgcttt
    10981 tcgtttgtag cgagctagct cttttcgccc gccgacacgc ttcacgttta gacgtgcgct
    11041 aaccgccacc gcgagatcac cgaaagtcca ccctggtgcg cgctaggtag aatcccgatg
    11101 tgcgacgaat gtcttggttt ctccttgtga tgatcctctc gtgccgtgac tctgggaatc
    11161 ggcagaccgc tttcgatcga aaagtgaagt gcgcacgcga agggcgcgag tggtttgcgc
    11221 gtgtttcgga gagtcagcga cgttataacg agacactcat caacccgcag tttgcataca
    11281 acgctgagct cacaacatgc ctgtgttact accgcttgaa ccatggccct gagcatagcc
    11341 tcgtcgtcac cgacatctcg acggacaaag aggttctggt ctggagagat gatcttggag
    11401 gtatcgacgc ccgacgatct ggtgcccgcc ggctaacact tgaggagttt gaccaccgca
    11461 tttctgatct tggatttcag ccctgagcgt ggccaaatca gaccaactcg ttcttgtggc
    11521 gggctaactc ctgtatcatt ccgctctcaa cgcaacgtgc ccgaaaacgt catcgcagtc
    11581 gctctgatcc ttcctgacct gacccgcagc gtcgcagaag agcatctcgc cgagctggaa
    11641 aaactgatcg agacagccgg gggcacggtt gtcgaccgcg tcttcgccaa gaggccgcgg
    11701 cccgacccgg ccacgtacgt cggcagcggc aaagccgagc agatcaaggc actcgtccag
    11761 cgcagcggcg ccacgctcat cgttttcgac gacgacctct cgcccgcgca ggtgcgcaac
    11821 ctcgagaaga tcaccgaggt caaggtcatc gaccggagcg gactgattct cgacatcttc
    11881 gccggccgcg cgcgcagccg cgaggcacgc acacaggtgg agctggcgca gctcgagtac
    11941 atgctgccgc gcctgacgcg ggcgtggacg catctggagc ggcaggccgg cggcatcgga
    12001 acgcgtggcg tcggcgaaac gcagctcgag ctcgaccgcc gcatcatccg caaccgcatc
    12061 gcccggctga aggaagagct ggagaaaatc gagcggacgc gcgagacgca gcgcaaatcg
    12121 cgcaacgccg tcttctccgt cgcgctcgtc ggctacacga atgcggggaa gtcgacgctc
    12181 ttcaaccggc ttaccagcgg ctccgccgac gcggccgaca agctgttcgc gacgctcgac
    12241 gccaaatcgg cgcgggccgc gttcgatatg ccgcgcgaga ccgtcgtgat cgacaccgtc
    12301 ggttttatcc gcaagctgcc gcatcacctc gtcgcatcat tccgctcgac gatggaagaa
    12361 gcggttgccg ccgatctggt gcttcacgtg atcgactcca gccatccgca gttcgaggac
    12421 cagcgcgcgg tcggcgagga agttctgcgc gagcttggcg tggtcatgga ccgcgtgatc
    12481 gaggtctaca acaaggccga tcgcaccgac atgttcgagc tccgccgaaa gaatgccgcc
    12541 agcgtctccg ccctcaccgg ccgcaacatc gagcgactca tcgacatcat ccgcgaacgt
    12601 gagctcgaaa acggggaggt catccagctc gagatccctc acaacgatgc acgggtgatc
    12661 gccaagctgc atgatgtcgc cgaggtgtat gaacagcgcg tcaccgaccg ggccatgatg
    12721 atcacggcct gggtaccgcg cgaccttctg catcttttcg aagcgtacgc ggccgtcagg
    12781 ttgcagccgg ccaaggtgag ttgatgcagc acaccgccgc tttgtgaccg gcgttacatt
    12841 gcgtcgtgat cgcgccgacg tctcgttttg ctccggtttc gtaatgtccg cagtgaggaa
    12901 cgagacctgc cgtgaatcgc cgagaccaac tggaacgcgc ttatgatggc cacgaccgcc
    12961 gcacgatagc gctcatgaaa atccgccctg ccgagttgcg cgaccgcggc cgcatcctgg
    13021 agatcctgat cgccactgcg cgcttcaccg acgtcgaagt gcgcgacgcc atggagctcg
    13081 tcgacaaagc caacgacgat ccgaatcaga gcgagtacat cgttcacgtt ctcgaagaac
    13141 cagccacggg cccgaggcga atggtgcagg gctatgtctg ctacggtccg accccgcaca
    13201 ccgagggcgt cttcgacctc tactggatcg cggtcgatcc gaagcagcag ggccagggaa
    13261 tcggccaact tctcctgcgt ttcgtcgaga atgaggtccg gcgccagcgc gggcggatgc
    13321 tgctgatcga aacgtcttcg aaggaaagct acgcgccgac ggtccggttc tatgaacgca
    13381 gcggttacga agagatctcc cggatcaagg atttctaccg tctcgcggac gataaagtcg
    13441 tctattgcaa gaagctgacg ccgagctgac atgagcccca acttcacact ctacgcagca
    13501 ctagcgctct acgccgcggg aaccgtgatg gcgttggtga ccctcttcac ccgcgagaag
    13561 cgcccacagc acatcgccct cggcatcatg atcgcgggct tcgtcgcgca cacggtgtgg
    13621 atcagcacga tctgcattcg gacgcatcac ccgccgctga ccaacctccc cgaggctgcc
    13681 gggttcatgg cgtgggtcgt gtttgccatc gagctggcgc ttttcatccg ctaccgcgtt
    13741 catgccgcgg cattcttcgt ttatccgctc gtgctcatgc tgctgacggt ctccgcagtc
    13801 gtgcacgagc cattcgtgcg gagcgcgact gccgtgtcgc gtctcttcat ctcgcacatt
    13861 cttctgacga cgatcggcgt ggctggcctg ctggtcggac tcgcgttcgg cgcgctggcg
    13921 tgggcacagg accgggcctt gaagtcgaag cagcgcggcg cactgtggga ttggattcca
    13981 agcctcagcg tgtgcaagac gctcagctat cgcgccctcg ccatcggttt cagcgtttac
    14041 accctcggca tccttgcagg gatcctctgg tcgtataaaa catcggccga actgatggat
    14101 gtccgcgtga aacaagtcgc cgctatggcg gcctgggtac tctttggcgt ggtgctgcag
    14161 gcgtacgtga acgataccta ccgcacgcgc cgcaccgcgg tgatcggcgc ctgcgcgttc
    14221 atagccatcg ttgtggcgat tctggggata catcatgtgt aaggggtgtc gggtgtcggg
    14281 agtcgggggt cggagaggaa ggcgtctccg gacgtctttt gcgcgcacag cttgtttgct
    14341 ccgacccccg acccccgacg tcctacgctc gtcctacgcg gaggccttat gcccctgatc
    14401 gctgtcggcg tgaaccatcg caccgcatcg gtcgatgtgc gggagcggct cagtgtggcg
    14461 acgtcgcaga tggcgccggc gctcgaagcg ttgcgcgagg ttgacggcat cgacggcgtg
    14521 gctgttcttt ccacctgcaa tcgcgtcgag gcgatcgtct ccgctaccgg cgaagacgtc
    14581 atcgagccga tcgtgaactg gctgacgcag cgcgcaggaa ccgaccgccc ggagctcgaa
    14641 aagcacatct acattctccg gcacggcgac gtgctcaagc atctcttccg cgtcgcctcc
    14701 gggctcgatt cgatgatcgt cggcgagccg cagattgccg gtcaggtgcg cgccgcgttc
    14761 atggccagcc gcgagctcgg ctcgctcgat tcgatcctct cccaggtctt cgatcagacg
    14821 atgcgcgtgg cgaagcgggt tcggacggac accggcatcg gcgagcttgc tgtctctgtg
    14881 ccgtacgccg cggtcgagct ggcgaagaag atcttcggtg atctgcacgg gctgcaggtt
    14941 ctgctcgtcg gcgccggtga aatcggcgag ctgacggcgg aacatctctc gtcgttcggg
    15001 ttagaacaga tcttcgtcgc caaccgctcg cacgaccgcg cggtggagct ggcggagcgc
    15061 tttcacgggc aggcggtgaa gttcgatggc atcgagccgt atctggcgac gtgcgacatc
    15121 gtcatcgcct cgacggccgc gccgcacttc gtcatcgaga gcagccacgt cgagcgcgcc
    15181 ctggccgcgc gcagaaagcg caacctcttc ctcgtcgatc tctgcgtgcc gcgcaacatc
    15241 gagccggcca tctcatcgat cgatggcgtg tatctctaca acatcgacga cctgcagcac
    15301 gtcgccgacg ccaatcgcga gatccggttg cagaaggcgg gcgaggccga gcagatcgtg
    15361 gagcgcgaag tggatgcgtt ccgccgccgc ctcgtggcgc aggacgcggt gccgacgatc
    15421 gtcgagctgc agcatcgcct cgaggacatc cgcgcggcgg agctggagaa gtgtctccgc
    15481 tagattggcc cgatgagccc ggagcagcgc gccgccatcg aacagctctc cacgcagatg
    15541 gtgaacaaga tccttcacta cccgatcctg cagctcaaag aagctgagga gccagccgag
    15601 cgcgagacgt tgcggaagac gatccgcaag atttttggat tgcgatgact cgcctggttc
    15661 gcatcggcac ccgtggcagc cagctcgcgc tctggcaggc aaatgaggtc gcaaacaagc
    15721 tgtcggagca g
//
LOCUS       DATLVW010000775         3166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_281479, whole genome shotgun sequence.
ACCESSION   DATLVW010000775 DATLVW010000000
VERSION     DATLVW010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281479"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1075
                     /locus_tag="VNN25_22730"
     CDS             83..1075
                     /locus_tag="VNN25_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXH94410.1"
                     /translation="MAKPFDRRSFMRLSAASLGAGALLRFMPFGTTVNGSTLGTLLGA
                     RNGEAPGPFSIVQLSDTHVGFQGPPDPLGTKAFERAVDAVNALDPQPDLILFTGDLTH
                     DSEDPAEHAKRFAAFKAISGRLRVKKRMHVPGEHDAALDGGTLFREHFGETHYSFDHR
                     GVHFVALDNVSAGKPIVGAEQIAWLKKDLARYPKTAPIVVFTHRPLFDLKAEWEWFTS
                     DGDEVMNVLAQYDAVTVLYGHIHRLNHHTIGKSQHYAARSLIFAFPEPPATSTADKKP
                     LPFDKTQPFRNLGIREITGNSARHALSVNDVELTSRELSGTEGVQQILKTGDFL"
     gene            1072..1434
                     /locus_tag="VNN25_22735"
     CDS             1072..1434
                     /locus_tag="VNN25_22735"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXH94411.1"
                     /translation="MISFNKSLIPSLLTLLLAADTVVVAQPGTPARVVEISAKKFEFT
                     PNVVTLKKDEPVTIHFTSTDRAHGLLVKAFKIDLDADLGKPADVTITPHEAGSFAAIC
                     DHYCGSGHGNMKMTFVVE"
     gene            1675..2316
                     /locus_tag="VNN25_22740"
     CDS             1675..2316
                     /locus_tag="VNN25_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94412.1"
                     /translation="MNLHLPNAIHFHLIFNHAPVFGELFAVGLLLAAAVVRSTTLLRT
                     ALVVVILTALGAIPVFFTGRAAADAIGKVEGIDQEAIGPHEEAAERFLFVAGAAGVAA
                     IVALVRPGRTAVVVAALLTLLSFGLAGWAANRGGLIHHQELRGPVTAQGAGFLQPATG
                     SAISARVAARKTQATNGASWNRVSRLAFYAFLKKQPSPRRARWAMANANTPTR"
     gene            2211..>3166
                     /gene="glpX"
                     /locus_tag="VNN25_22745"
     CDS             2211..>3166
                     /gene="glpX"
                     /locus_tag="VNN25_22745"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HXH94413.1"
                     /translation="MESSLEIGFLRVSEKAAIAAARTMGYGKRKYSDQVAVESMREEL
                     NRLKMRGTVVIGEGERDKAPMLYVGEPLGRGWTEGEIYPEIDIAVDPLEGTNLCATGS
                     ANSIAVLAASEKGGLLNAPDVYMDKIVVGPTAAEHVSLTASVHENLNQIAAAFQRDVD
                     ELTIVVLDRERHHDLIAQIREAGARIKLITDGDLSAGIAAAVRGTGIHAAMGIGGAPE
                     GVLTASAMRCLNGRMQGRLKPLEKWQEERLRSMGYVDGEKVYDTAELASGNDIIFCAT
                     GVTDGDLLRGVRFFGGGIRTSSLFMSLKSNTIRFVDTIHREEG"
BASE COUNT          614 a         1115 c          903 g          534 t
ORIGIN      
        1 cccgactccc gactcccgac tcccaccccc cgggatgaaa acctcccttc gccagttaca
       61 cagccagaaa ggacaacaac tcatggcaaa accattcgat cgccgctcgt tcatgcgcct
      121 ctcggccgcc tcgcttggcg cgggcgcgct cctgcgattc atgcccttcg gcactaccgt
      181 caacggttcc accctcggca cccttctcgg cgcacgcaac ggcgaagctc ccggtccgtt
      241 ttccatcgtg cagctcagcg atacgcacgt cggatttcag ggaccgcccg accccctcgg
      301 cacgaaagca ttcgagcgcg ccgtcgacgc agtcaacgct ctcgatccgc aacccgacct
      361 catcctcttc accggcgact tgacccacga cagcgaggat cccgccgagc acgccaagcg
      421 cttcgcggcg ttcaaagcga tctccggccg ccttcgcgtc aagaagagga tgcatgtgcc
      481 cggcgagcac gatgccgcgc tcgatggcgg gacgctcttc cgcgaacact tcggcgagac
      541 ccactactcg ttcgatcatc gcggtgtcca cttcgtcgcc ctcgacaacg tctcggccgg
      601 caagccgatc gtcggcgccg agcagatcgc ctggctcaag aaggatctgg cccgctaccc
      661 taagaccgcc ccgatcgtcg tcttcaccca ccggccgctc ttcgatctga aggcggagtg
      721 ggagtggttc acaagcgacg gcgacgaggt gatgaacgtc ctcgcgcagt acgacgcagt
      781 gaccgttctc tacggccaca tccaccgtct caaccaccac accatcggca agtcgcaaca
      841 ctacgccgcg cgctcattga tcttcgcttt ccccgagccc cctgccacat cgactgccga
      901 caagaagcca cttcccttcg acaagaccca acccttccgc aacctcggca tccgcgagat
      961 caccggtaac agcgcacgtc acgcgctctc cgtcaacgac gtggagctga cctcgcgcga
     1021 gctctccgga accgaaggcg tccagcaaat cctcaaaacg ggagatttcc tatgatcagc
     1081 ttcaacaagt ccctcattcc ctcgttgctc acgctactgc tggccgccga cacggtcgtt
     1141 gtggcgcagc ccggtacgcc ggcgcgcgtc gtcgagatca gcgccaagaa gttcgagttc
     1201 acgccgaacg tcgtgacgct caagaaagac gagccggtga ccatccactt caccagcacc
     1261 gaccgcgcac acggcctcct cgtcaaagca ttcaagatcg acctcgatgc cgacctcggc
     1321 aaacccgccg acgtgactat caccccgcac gaagccggca gcttcgccgc gatctgcgac
     1381 cactactgcg gctccggcca cggcaacatg aagatgacgt tcgtggtgga gtgacaagtg
     1441 gagtgcaggc gtctcgcctg cggccgatgg gcgtctcgcc cagcggtaaa cccacggcta
     1501 tacgaagaac ggcccggcgt ccacgcaaca cacggcgaat gctgtggacg ccgggcagac
     1561 taacaagcta acgcatggga atgcggacga agacgtcaac cctcacgcca cacacgccga
     1621 ggcgagacgc ctcgccgccg caggcgagac gcctgcactc cttgggagca aaatatgaac
     1681 ttacatctcc ccaacgccat ccacttccac ctcatcttca atcacgctcc ggtattcggc
     1741 gagctcttcg cggtggggtt gctgcttgcg gcggcggtgg ttcgcagcac tacgctcctg
     1801 cgcaccgcgc tcgtcgtcgt gatcctcacc gcactcggcg cgattccggt cttcttcacg
     1861 ggacgcgctg ccgcagatgc gatcggaaag gtcgaaggca tcgatcagga agccatcggt
     1921 cctcacgagg aggctgcgga gcgtttcctc ttcgtggcgg gggcggcggg cgtcgcggca
     1981 atcgttgctc tggtccgtcc cggtcggacc gccgtcgtcg tcgctgcctt gctgacgctc
     2041 ctctccttcg gcctggccgg atgggcggcc aatcgcggtg ggctgatcca tcatcaggag
     2101 ttgcgaggac ccgtcactgc gcaaggtgcc gggttcctcc aaccagctac cggctccgct
     2161 atatctgcta gagttgccgc ccggaaaact caggcaacga acggagcgtc atggaatcga
     2221 gtctcgagat tggcttttta cgcgtttctg aaaaagcagc catcgccgcg gcgcgcacga
     2281 tgggctatgg caaacgcaaa tactccgacc aggtagcggt ggagtcgatg cgcgaagagc
     2341 tgaaccggct caagatgcgc gggacggtcg tgatcggtga gggagagcgc gacaaggctc
     2401 cgatgctcta tgtcggcgag cccctcgggc gcggctggac tgaaggcgag atctatcccg
     2461 agatcgacat cgcggtcgat ccgctcgaag gaacgaacct ctgcgccacc ggctcggcaa
     2521 actccatcgc cgtcctcgcg gcctccgaaa aaggcggtct gctcaacgcg cccgacgtct
     2581 acatggacaa gatcgtcgtc ggcccgactg ccgccgaaca tgtaagcctc accgcctctg
     2641 tacacgagaa tctcaaccag atcgcggcgg cgttccagcg cgatgtcgat gagctgacga
     2701 tcgtcgtcct cgaccgcgaa cggcatcacg acctcatcgc ccagattcgc gaggcgggcg
     2761 cgcgcatcaa actgatcacc gacggcgatc tctccgccgg catcgccgcc gcagtgcgcg
     2821 gcaccggcat tcatgcggcc atgggcatcg gcggcgcgcc cgagggcgtg ctgacggcat
     2881 cggcgatgcg ctgtctcaac ggccggatgc agggccgcct caagcctctc gaaaagtggc
     2941 aggaagagcg actgcgctcg atgggctacg tcgacggcga aaaggtttac gacaccgccg
     3001 agttggcatc cggtaacgac atcatcttct gcgcgaccgg cgtaaccgac ggcgatctcc
     3061 tgcgcggcgt ccgcttcttc ggcggcggaa tccgcacgtc atcgctcttc atgtcgctca
     3121 agtcgaacac gatccgcttc gtcgacacga tccaccgcga agaggg
//
LOCUS       DATLVW010000776         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_568390, whole genome shotgun sequence.
ACCESSION   DATLVW010000776 DATLVW010000000
VERSION     DATLVW010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568390"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VNN25_22750"
     CDS             <1..373
                     /locus_tag="VNN25_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94414.1"
                     /translation="GNADRFLPYADKTFSRVLSITARMNVSELRRVVRDDGRLLVAIP
                     APDDLVELRGAGRDRVTRTTADFGAAFTLVEQRRASTVADLDRDAVHDVLLSIYRPRS
                     IEKVSAARVTFSLDLLLFAPR"
     gene            394..1341
                     /locus_tag="VNN25_22755"
     CDS             394..1341
                     /locus_tag="VNN25_22755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH94415.1"
                     /translation="MAAPYFSVVIPTYKRLPMLLRVLDSLEEQQDAPEFEVIVIDDGS
                     GDDTAAVMQARRAKHYALTFQSQPNSGPGRARNRGVALATGRYVLFIGDDTVPEPHFL
                     AQHARSHGEAGDDPHLACLGYTGWPAGERVTAFMDYINDFGLQFGYKLIRDGEIVPFN
                     FFYTSNISIDRQLLGLEPFDTTFPAAAWEDIELAFRLDALGLKIRYNAQAVTRHYHPM
                     TVDSFARRQYTVGRSGAIFYRKHPELAGFLGVHELETRTLANDKRLDHLRRRARLGER
                     FRLLATNRVFETLMREHYLRGLRDGLAAASPASNDESSG"
     gene            1355..1981
                     /gene="tmk"
                     /locus_tag="VNN25_22760"
     CDS             1355..1981
                     /gene="tmk"
                     /locus_tag="VNN25_22760"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXH94416.1"
                     /translation="MFITFEGIEGSGKSTQLRRVAESIPDAVVTKEPGGTPLADRIRA
                     VLLDSSSQMDPVAEVLLFAASRRENTLKVIRPALDRGGIVLCDRFTDATLAYQGFGRL
                     LNLDQLRALNAWATNSLVPDLTLLFDLPEQTGLTRARSRNADASQDEGRFEAEDLRFH
                     QRVREGYLTLAAAEPSRFALIDAVGDVDDVYARVEGVLSARLPRYFGE"
BASE COUNT          352 a          689 c          605 g          378 t
ORIGIN      
        1 cggcaatgcg gaccggttcc tgccgtacgc ggacaagacc ttctcgcgcg ttctgtcgat
       61 caccgcacga atgaacgtgt ccgagctccg ccgcgtcgtg cgcgatgacg gacggctgct
      121 cgtcgcgatt ccagccccgg atgatctcgt cgaactgcgc ggcgccggcc gcgaccgcgt
      181 cacacgaacc acggcagatt ttggagcagc gttcaccctc gtcgaacaac ggcgagcgtc
      241 gaccgttgcc gatctcgatc gagacgccgt ccacgacgta ctgctgtcga tctaccgccc
      301 gcgcagcatc gagaaggtga gtgccgcacg cgtcaccttc agtctcgatc ttctgctctt
      361 cgcgccgcgc tgagcgctac aatccgccgc ccgatggccg cgccctactt ttcagtcgtc
      421 atcccgacgt acaaacggct gcccatgctg ttgcgcgtgc tcgactctct cgaagagcag
      481 caggacgcgc cggagttcga agtcatcgtg attgacgacg gatcgggcga cgacaccgcg
      541 gccgtcatgc aggcgcggcg cgcaaagcac tacgccctca cgttccagtc gcagccaaac
      601 tccggcccgg gaagagcccg caatcgcggc gtcgcgctgg ccaccggccg ttacgtcctc
      661 ttcatcggcg acgacacggt ccccgagccg cacttcctcg cgcaacatgc ccgatctcac
      721 ggcgaggccg gcgatgaccc gcacctcgct tgcctcggct atacgggttg gccggccggc
      781 gagcgcgtca ctgccttcat ggattacatc aacgacttcg gattgcagtt cggctacaag
      841 ctcattcgcg acggcgagat cgtcccgttc aactttttct acacgtcgaa catctcgatc
      901 gacaggcaat tgctcggcct ggagccattc gatacgacgt tccccgcggc agcatgggaa
      961 gacatcgagc tcgcttttcg cctcgacgcc ctcggcttga agattcgcta caacgcgcaa
     1021 gcggtcaccc gtcactacca tccaatgacg gtcgattcgt tcgcgcgccg gcaatatacg
     1081 gttggccgct ccggcgcgat cttctatcgc aagcatcccg agctggccgg cttcctcggc
     1141 gttcacgaac tggagacgcg aaccctcgcc aacgacaagc gtcttgatca tctccgccgc
     1201 cgcgcccgcc tcggcgaacg attccgcctc ctggccacga accgcgtgtt cgagacgctg
     1261 atgcgcgagc actacctgcg cggtctgcgg gacgggctgg ccgctgcgtc gccggcttcg
     1321 aacgacgagt cctcgggata gagttcacag aacaatgttc atcacgttcg aaggcattga
     1381 aggctccggc aagtcgacgc agctccggcg cgtcgcggag agcattccgg acgcggtcgt
     1441 gacgaaggag ccgggaggga cgccgcttgc cgaccgcatt cgcgcggtgc ttctcgacag
     1501 cagctcgcag atggatccgg tagccgaggt gttgctgttt gcggcatcgc gccgggagaa
     1561 cacgctcaag gtcatccgcc ccgcgctcga ccgcgggggc atcgtcttgt gcgaccgttt
     1621 caccgacgcg acgctcgcgt atcaaggttt cggacgcctg ttgaatctcg atcagttgcg
     1681 cgccctgaat gcgtgggcca ccaactcgct ggtccctgat ctcacactcc tcttcgattt
     1741 gcccgaacag accggtctca ctcgcgcccg ttcgcgcaac gccgacgcgt cgcaggacga
     1801 aggtcgattc gaagctgagg atcttcgctt tcatcaacgg gtacgagagg gatatctaac
     1861 gctcgccgcc gcggagccct cgcgttttgc attgattgat gcagtcggcg atgtcgacga
     1921 tgtctacgcg cgcgtcgaag gcgtgctctc cgcaagattg ccgaggtact tcggtgagtg
     1981 acgcggcgcg gctgctgctg gagaccgcac agcgaggcga gttg
//
LOCUS       DATLVW010000777         5172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_568394, whole genome shotgun sequence.
ACCESSION   DATLVW010000777 DATLVW010000000
VERSION     DATLVW010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568394"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VNN25_22765"
     CDS             complement(<1..402)
                     /locus_tag="VNN25_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94417.1"
                     /translation="MSSPVLDFSPVAVTFQAIGTLLLALVLGQIARTFGWRYVKRWAL
                     AFVSMFCAIVAVRVYIATSQPWPWWLVYILAQWVFLALLFAGCRDLIDNPLPLKFLGF
                     ATPMTLAASILIVIAAPEFGALFMIEAGIVSV"
     gene            complement(550..837)
                     /locus_tag="VNN25_22770"
     CDS             complement(550..837)
                     /locus_tag="VNN25_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94418.1"
                     /translation="MSWRIDKQANGKFAIYSSRVDDYIVIDADEAEIERIYTEKGIKV
                     YLASARAQMTTRVTPVAPGGEAKIDASRQRGATPKEGVVPIGVTGFTMSDE"
     gene            complement(979..2487)
                     /locus_tag="VNN25_22775"
     CDS             complement(979..2487)
                     /locus_tag="VNN25_22775"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXH94419.1"
                     /translation="MRRIPIIIALTFISLTTSVAFGQTLGAVLTASQETPPTTTPGFG
                     NATVTFDSTRQNITVTITVSNLGAPINNFHIHEAAAGVPGPVVENLIGLGGTFVNGTM
                     TGTFPIAADVALRMVQNPANFYVNVHTTQFPGGAVRGQLAFVSGGPVTYAAELRGANE
                     VPPTGSSAFGGAFITFDPINNNLTWEVNTSGIVSPTLSHIHKGVAGQNGNVLINFATG
                     PSQIPNGRTNGTIAVGSLDPSDLTALASASTATGYYVNVHSSAFGGGEIRGQLAAANE
                     YDVAVAGKVTNFVTDVRVFNPSYTQSTVALIEFMTGGTTAAASTVVNIAPRGTAVLDD
                     VAGSSGLNVAGSIGGLRVSSVSQLAVTSRIYADLRSSAKGTLGLFVPAVARGGGLRRG
                     VLPQLSNTAGGYRTNIGFFNPNPEPVTLRLELHDATGTLVGQNTLTLGPLSQQQNAIG
                     VYFPGVDLSNSANLTMSFDASALILGYASVNDNVSADPSFVAALPDSGVATN"
     gene            complement(2744..>5172)
                     /locus_tag="VNN25_22780"
     CDS             complement(2744..>5172)
                     /locus_tag="VNN25_22780"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH94420.1"
                     /translation="PTRQDCRRTGADGSVVFRTSEGPHSVFVTGDEVAPKRVPSVTLT
                     ARSEPLVVEVDRGVTISGRVVHADGTPVAGAVVELPRSPIAPRNATADSDGAFTLVGI
                     AAGPTSLTAVSSDRRLSSAPVPVTAPAKNVTITMPHGARIEGRVVDRATKQPVTDFTV
                     SVPSRGRPGPSPTPQQVHADDGSYAIDNVAPGTFQIAVTATGFVTATRTDITAEEGKT
                     VSGIDVQLDRGASITGRVTSGGAPVAGAQVAVTANRMPFFGGTTTDADGNYTLDGVAE
                     GERTLQFTRTGYIVLHKTVEVTAAKDLHLDVELDHGAEIRGRVTDRAGHNIPGANVAI
                     AGDRSGPSNMTQTDLDGSFVMQGLATGSYRVTARKEGYVSEDVNDVTVPQTQPLTFAL
                     DHGATITGRVTGLAPEELAQVVVVASGGTSRNQTPVDSAGNFTLHGMPDGRIRVDAML
                     STAGRNRTALPKIIVVENGAAPPVELDFSEGITISGHVTHAGAAVSMASIIFFPTPQT
                     AQKNAAAQMGQGGGSGMISPDGSYVVTLMMPGDYNVRINGQNLAYQTTYTATSSSTFD
                     IDIHGAMLRGHVVDAATGAPLANVQVMVFSRAVQANGSATTDSDGHFAIDALIDAIYD
                     LRATREPYSVATQQVTVSGGSAPEVEVRLEQAPAVTFHVTDSVTGAPVDASVMVTTDQ
                     HANPAQAQRVESGVLKAWLKPGNYIAAVFGRYYIGKTTNFTTPPADVQITLVRGGQMI
                     IEAKSAQQVRLDQLGGRLPGPVHPGINGPYPTIAPGSYLLSVLDNSGSVVRTVPVVIV
                     AGETTTVQLP"
BASE COUNT          936 a         1582 c         1691 g          963 t
ORIGIN      
        1 aacgctgacg atgcctgcct cgatcatgaa gagcgcgccg aattcgggag cggcgatgac
       61 gatcagaatt gatgctgcca gcgtcatcgg cgtcgcgaat ccgaggaact tgagcgggag
      121 aggattgtcg atgagatcgc ggcagccggc gaacaagagg gcgaggaaaa cccactgcgc
      181 gaggatataa acgagccacc agggccacgg ttgcgaagtg gcgatgtaga cgcgcacggc
      241 cacgatggcg cagaacatcg acacgaatgc cagcgcccag cgcttcacgt aacgccagcc
      301 gaacgtccgc gcgatctgcc cgagaacgag ggcgagcagc agcgtcccga tcgcctgaaa
      361 cgtcacggcg acgggcgaga aatcgagtac cggcgaggac atgcgctctg agaaaccgaa
      421 tgttggacgt tgaatgtttc gtgttgaatg gacggcggcc tcgggttcgt tttcccgcga
      481 ccaggtgtca cgctcacgac ttcagttcaa cagtagaccg cgaacccgtt gcgatggatg
      541 taacaccaat cactcatcgc tcattgtgaa cccagtcacc ccgatcggca caacgccctc
      601 ttttggcgtg gcgccgcgct ggcggctggc atcgatcttc gcctcgccgc cgggagcgac
      661 gggcgtgaca cgcgtcgtca tctgcgcgcg ggcgctggcg aggtagacct tgatgccttt
      721 ctccgtatag atgcgctcga tctccgcctc gtcggcgtcg atgacgatgt agtcgtcgac
      781 gcggctggag tagatcgcga acttgccgtt cgcctgcttg tcgatgcgcc aggacatggt
      841 gcatagagta gcggatcgac tgccacgaac gtgaccgact tgtcgatcgg ctacgttgcg
      901 actcctgtcg cctggtgagg ctggatcgaa agccgcgccg gatacgagtc cggcgcggct
      961 tgctcgaatc gatgaccgct agttcgtcgc gacgccgctg tcgggcagcg cagcgacgaa
     1021 gctcggatcg gcggagacgt tgtcgttgac ggaggcgtag ccgaggatca acgccgaagc
     1081 gtcgaagctc atcgtcaggt tggccgagtt ggagagatcg actccgggga agtaaacccc
     1141 gatcgcgttc tgctgctgcg atagaggtcc gagcgtcagc gtgttctggc cgaccagtgt
     1201 tccggtggcg tcatgcagct cgagacgtag cgtgaccggc tccggattcg ggttgaagaa
     1261 gccgatattc gtgcggtaac cgccggccgt gttcgagagc tgcggcagga ccccgcggcg
     1321 cagtccaccg ccacgcgcta ccgcggggac gaacaagccg agggttcctt tggcggatga
     1381 acgcagatcg gcgtagatgc gggaagtcac tgcgagctgc gacacactcg agacccgcag
     1441 cccgccgatc gagccggcga cgttgaggcc ggacgatccg gcgacatcgt ccagaacggc
     1501 ggtaccgcgc ggcgcaatgt tgaccaccgt cgatgcagcg gcggtggtgc cgcccgtcat
     1561 gaactcgatc agcgcgacgg tgctctgcgt gtaggaaggg ttgaagaccc gcacgtcggt
     1621 aacaaagttc gtgaccttgc cggcgacggc aacatcgtac tcattcgcag cggcgagctg
     1681 ccctcgtatc tcaccgcctc cgaaggcgct ggagtggacg ttgacgtagt agccagtagc
     1741 ggtcgatgca cttgccaacg cggtcaggtc ggatggatcg aggcttccaa ccgcgatcgt
     1801 cccatttgtg cggccattcg ggatttgcga cggtcccgtc gcgaagttga tgagcacgtt
     1861 cccgttctgg ccagcgacgc ctttgtgaat gtgggacagc gtcgggctga cgatcccgct
     1921 cgtgttgacc tcccaggtga ggttgttgtt gatcggatcg aacgtgatga acgcgccgcc
     1981 gaacgcgctg ctgccggtcg ggggcacttc gttggcgccg cgcaactccg cggcataggt
     2041 caccggaccg ccgctcacga atgcgagttg gccgcgaacg gcgccgccgg gaaattgcgt
     2101 cgtatgcacg ttcacataga agttggcggg gttctggacc atcctgagcg cgacatcggc
     2161 ggcaatcgga aacgttcctg tcatcgtgcc gttgacgaag gttccgccga gtccgatcag
     2221 attctcgaca accggtccag gcacgcccgc tgcggcttcg tggatgtgga agttgttgat
     2281 tggagcgccg aggttgctga ccgtgatcgt gacggtgatg ttctggcgcg tcgagtcgaa
     2341 cgtcaccgtg gcattaccga agcctggggt ggtggtgggc ggcgtctcct gcgatgcagt
     2401 cagtacagct ccaagcgttt gtccgaaggc gacggatgtg gtgagggaaa tgaacgtgag
     2461 agcaatgatg atcgggatac ggcgcatgtt ttcctcctca agcgagatca aaaggcgcga
     2521 agtattcaaa cccgcggctt ggattagtcg gcttggcgcg atgcacacct caagcccgcc
     2581 cgctctcaga ctgtgcgctg catcacaaag tcgtctcctg atggttcgtg gcgttaacca
     2641 cgatcgtatc cgctggcgat agcggatcgt cgattgcgag gagacacagc gccagcaagc
     2701 ggcatcagac gatgcccgtg tgactcgacc tccatcgccg ctatcagggg agttggaccg
     2761 tcgtagtctc ccctgccacg atcacgaccg gcaccgtgcg cacgacgctg ccactgttgt
     2821 cgaggacgga caagaggtat gacccgggcg cgatcgtcgg ataaggtccg ttgatccccg
     2881 ggtgtacagg accaggaagg cgcccgccga gctggtcgag acggacctgc tgcgcgctct
     2941 tggcctcgat gatcatttgt ccgccgcgca cgagggtgat ctgcacgtcg gccggcgggg
     3001 tcgtgaagtt cgtggtcttg ccaatgtagt agcggccgaa caccgcggcg atgtagttgc
     3061 ccggcttcaa ccaggctttg aggacgccgc tttccacgcg ctgcgcctgg gctggattcg
     3121 catgctgatc ggtagtgacc atgaccgaag cgtcgaccgg tgcacccgtg acggagtcgg
     3181 tgacatgaaa cgtcacggct ggcgcctgct ccaaccggac ctcgacttcc ggagcgcttc
     3241 cgccggacac ggtgacctgt tgcgtggcga cggagtatgg ctcgcgcgtc gcacggaggt
     3301 cgtagatcgc atcgatcaga gcatcgatcg caaagtgacc atccgaatcg gtcgtagccg
     3361 atccgttagc ctgcacggca cgcgagaaaa ccatcacctg cacgtttgcc agcggagcac
     3421 cggtagctgc gtcaacgaca tgaccgcgca gcatcgctcc gtgaatgtcg atgtcgaatg
     3481 tgctgctact ggtggcggtg tacgtcgtct ggtacgcgag attctggcca ttgatgcgaa
     3541 cgttgtagtc gccgggcatc atcagtgtga ctacgtagct gccatccggg gagatcattc
     3601 cgctgccgcc gccctgtccc atctgtgcgg ctgcattctt ttgcgccgtt tgcggcgttg
     3661 gaaagaaaat gatgctggcc atcgaaacgg ccgcgcccgc gtgggtcacg tgaccgctga
     3721 tagtgatccc ttcgctgaag tcgagctcga ccggcggcgc tgcgccattc tcgacgacga
     3781 tgattttcgg aagcgccgtc cggttccttc ctgccgtgga gagcatcgcg tccacgcgga
     3841 tgcgcccatc aggcataccg tgcagcgtga agttgccggc cgaatccaca ggagtctggt
     3901 ttcgtgacgt gcctccggag gccaccacga ctacctgcgc caactcctcg ggcgccagac
     3961 cggtaacccg ccccgtaatc gtggctccgt ggtcgagcgc gaaggtgaga ggctgagtct
     4021 gcggaacggt aacgtcattc acatcctcgc tgacgtaacc ctctttccgc gccgtaacgc
     4081 ggtagctccc ggttgccaga ccctgcatga cgaagctgcc atcgagatcc gtctgcgtca
     4141 tgttgctcgg cccactccgg tcaccggcga tcgcaacgtt ggcgccggga atgttgtgac
     4201 ctgcgcggtc cgtgacgcgg cctcgtatct cggcaccgtg gtcgagctcc acgtcgagat
     4261 gcagatcctt cgccgccgtg acctcgactg tcttgtgcag aacgatgtat cccgtccgcg
     4321 taaattgcag cgtacgctcc ccttccgcaa ccccatcgag ggtgtagttc ccatccgcgt
     4381 cggtcgtcgt gccaccgaag aacggcatcc ggttcgcggt gaccgctacc tgcgcgccgg
     4441 ccaccggtgc accacccgac gtgacccgcc cggtgatgct cgcaccgcga tcgagctgca
     4501 catcgattcc gctcaccgtc tttccctcct cggcggtgat atcggttcgc gtagccgtaa
     4561 caaagccggt ggcagtgacg gcgatctgaa atgttcccgg tgcgacgttg tcgatcgcgt
     4621 agctgccgtc gtccgcgtgg acctgctgcg gcgtcggact gggtccaggc ctgccgcgcg
     4681 aaggaacgga gaccgtgaag tcggtgaccg gctgcttcgt cgcccgatcg accacgcgcc
     4741 cctcgatgcg cgcgccgtgc ggcatggtga tcgtcacatt ctttgccggt gccgtgacgg
     4801 gaacgggagc cgacgacaac ctacggtcgg aggagaccgc cgtgagcgat gtcggccccg
     4861 ccgcaattcc gacgagcgtg aaggctccat ccgaatcggc cgttgcattg cgcggcgcga
     4921 tcggagagcg cggcaactcg acgacggcgc cggcgactgg cgtgccgtcg gcgtgaacga
     4981 cgcgcccgct gatcgtgaca ccacgatcga cttcgacaac gagcggctcc gagcgggcgg
     5041 tgagcgtgac actcggtaca cgtttcggcg caacctcgtc gccggtgacg aagacagagt
     5101 gcgggccctc ggatgtccgg aagacgaccg agccatcagc accggtgcgg cggcaatcct
     5161 gacgcgtcgg cg
//
LOCUS       DATLVW010000778         5230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_321470, whole genome shotgun sequence.
ACCESSION   DATLVW010000778 DATLVW010000000
VERSION     DATLVW010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321470"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..1598)
                     /gene="dinB"
                     /locus_tag="VNN25_22785"
     CDS             complement(513..1598)
                     /gene="dinB"
                     /locus_tag="VNN25_22785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXH94421.1"
                     /translation="MRKILHIDMDAFYASVEQRDRPELRGKPVIVGWPGERSVVCAAS
                     YEARKFGVHSAMPATRAMRLCPEGTFIHPDFEKYRAVSKHIREIFGRHTPLVEPLSLD
                     EAYLDVTEELTGIPTATETAETIRREIRSETHLTASAGVAPNKFLAKIASDWRKPDGC
                     FVIRPHQVQQFLMPLPVRKIPGVGPATEKVLTDMGIATVGDLHGFSSEQLVARFGKWG
                     TRLWELARGIDESPVVPSQKRKSWSSENTFPTDITIEEVAAYITAEAEKLWKALSDKQ
                     LTGRTVTVKLRTGEFKTATRRLTPESPPSSGDDLARIGTELLTRFDFPPNSRYRLAGI
                     GMSNFVDEEDEEEISDGLFPGSSLVSS"
     gene            1644..2750
                     /locus_tag="VNN25_22790"
     CDS             1644..2750
                     /locus_tag="VNN25_22790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXH94422.1"
                     /translation="MTIANIPTPAFLVDRTIVEQNCLRMRDKAQASGVLFRPHVKTHK
                     TIEIGRMQHGGGTGPITVSTMAEAEFFANAGFGDITYAMPIAPEKLARAAALAARIER
                     FNILIDSAAALHAVEEFCAAHDDAFDVFLKVDCGYHRAGVDPDDPDSARLAIAISRSP
                     AVHFQGLLTHAGHSYNAHDVDEIRRIAAEETSSLTRFRALIENEGLAGVVRSSGSTPT
                     TSVVETFTDTDEVRPGNYVFFDAFQSTLGSCTRKDVAVSVLATVVGSYPERGEAIIDA
                     GALALSKDTSPDHLDPTFGYGLVCDVALHPLDARLVALSQEHGKLRMSAPPPVGTRLR
                     IVPNHSCLTAAMYDRYHVIDGDAIVDEWRPVRGW"
     gene            complement(2927..3787)
                     /locus_tag="VNN25_22795"
     CDS             complement(2927..3787)
                     /locus_tag="VNN25_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94423.1"
                     /translation="MAPTTRSVAPTSDCVTRTLSSVPVTVSAKRRISVPFASTQMNVT
                     RTASAAFTAEFESEEELREEHRTNLSMGGLRLVTAETVALNATLLVTLRGPWAGESFA
                     RATVVALLPDGIALSIDGNSDELLSRLMAKPLGDPEPPTEEAPEQEETTEKRENVWDR
                     IRGLSQMEKLLLAVKADRSERALLLQDNDPRVLLSLLRNPRLTVDEVARLAKSTFLNY
                     QIADVIMKTTQWMSVLDVRLGLMHNAKTPPAFALRILPTLPESEIRSIARAGSSMALK
                     TAALRLLQGR"
     gene            complement(3920..4390)
                     /locus_tag="VNN25_22800"
     CDS             complement(3920..4390)
                     /locus_tag="VNN25_22800"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HXH94424.1"
                     /translation="MQTLPAEYATRLGGIVLATETIPVHILNALTAEGAKAKGETVEQ
                     FARRAGREGASDAVKGIYRFFALILTPAALLSKASSMWSTLYNRGQMRIENQTDRGAT
                     IKLLDFPSETAGCARITGWIERMAELTGVKDVRVQQTQCYAKGAPHCEWNVTWQ"
     gene            4618..>5230
                     /locus_tag="VNN25_22805"
     CDS             4618..>5230
                     /locus_tag="VNN25_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94425.1"
                     /translation="MDEQTGGAAEASAAIAEAESHNEPKSGAKDRLEELCVNTIRFLA
                     IDGVEKAKSGHPGAPMGMADMAYVLWTDFMRHDPKAPSWQDRDRFVLSAGHASMLLYS
                     LLHLTGYPEMTIDQLKSFRQWGSKTAGHPEYGDAPGIEVTTGPLGQGFANGVGMGIAS
                     KMMAARFNTEDPFKPVQNWIYVICSDGDLEEGISSEAASLAGHL"
BASE COUNT          942 a         1687 c         1621 g          980 t
ORIGIN      
        1 cggcgtagga cggtcgaagg atctcaaaat gcgatgatct cgttcgagcg cctcttgatc
       61 acgaccgctc gcgctggacg aacacgattc gcgaagtttg agatccttcg acgtcctccg
      121 ccgccgagca agcactgcgc aaggatcgcg attcgcgagg acggctccgg cagtctcagg
      181 atgacaccaa tctcgattgg acgatcactc gagaggacgc gattgctgtc atgccgaacg
      241 gccgcagatt gtgaacgatg cgcacaaaac gtgttgtgtc atcctgagcc gccgaagccg
      301 ccgcaagatt cgctgacgtt gcatccttca tagataggcg gcgtaggacg gtcgaaggat
      361 ctcaaaatgc gacgatcctg ttcgagcgtc tcttgatcac gatcgctcgc gctggacgaa
      421 catgatctgc gaagtttgag atccttcgac gtcctccgtc gccgagcaca cactgcgcaa
      481 acatcgcgat tcacgcgggc gactccggcg tctcaggatg acaccaagct ggaacccgga
      541 aacaagccgt cgcttatctc ttcttcatcc tcctcatcga caaagttcga catcccgatt
      601 cccgctaacc ggtagcggct gttcggcggg aagtcgaagc gggtgaggag ctcggtgccg
      661 atgcgagcga gatcgtcgcc gctcgatggc ggcgattcgg gagtcagccg gcgcgtggcg
      721 gtcttgaatt cgccggtgcg cagcttgacg gtcacggtgc ggccggtcag ttgcttgtcg
      781 ctgagcgcct tccatagctt ctccgcctcg gcggtgatgt acgcggccac ttcttcgatc
      841 gtgatgtcgg tggggaaggt gttctcactc gaccacgact tccgtttctg cgatggcacg
      901 accggtgatt cgtcgatacc gcgggccagc tcccagaggc gcgtgcccca cttgccgaag
      961 cgggccacga gctgctcgct gctgaaaccg tgaaggtcgc cgacggtggc gatgcccatg
     1021 tcggtcagca ccttctccgt ggccggcccg acgcccggga tcttgcgcac cggcagcggc
     1081 atgaggaact gctgcacctg gtggggacgg atgacgaagc aaccgtccgg cttgcgccag
     1141 tcgctggcga tcttcgccag gaacttgttc ggggccacgc ctgccgaggc ggtgaggtgg
     1201 gtctcggagc gaatctcgcg gcggatcgtc tccgccgtct ccgttgccgt cggaatcccc
     1261 gtcaactcct cagtgacatc gagataggcc tcatcgagcg agagcggctc gacgagcggc
     1321 gtatgccgtc cgaagatctc gcggatgtgt ttcgacaccg cccgatactt ctcaaagtcc
     1381 ggatgaatga acgtcccctc ggggcacaga cgcatcgcgc gcgtagccgg cattgccgaa
     1441 tgcacaccga acttgcgcgc ttcgtacgac gccgcgcaaa ccacgctccg ttcgcccggc
     1501 catccgacga tgaccggctt cccgcgcagc tccggccggt cgcgttgctc gaccgaggcg
     1561 tagaacgcgt ccatgtcgat atgaaggatc tttctcattg gatttcttcg cgtcttcgcg
     1621 ccttcgcgtg gcaacatctt cgcgtgacca tagcgaacat acccactcca gccttcctcg
     1681 tcgaccgcac cattgtcgag caaaactgcc tgcgcatgcg cgacaaggcc caggcctcgg
     1741 gggtcttatt ccggccgcac gtgaagacgc acaagacgat cgagatcggg cggatgcagc
     1801 atggcggcgg caccggtccg atcaccgttt cgaccatggc cgaggcggag ttcttcgcga
     1861 acgcgggatt cggcgacatc acctacgcca tgccgatcgc gcccgaaaaa ctggcgcgcg
     1921 cggcggcgtt ggcagcacgg atcgagcggt tcaatattct catcgacagc gccgcggctc
     1981 tacacgcggt agaggagttt tgtgcggcgc atgacgacgc attcgacgtc tttctcaagg
     2041 tcgactgcgg ctatcaccgt gccggcgtcg atcccgacga tcccgacagc gcccggctgg
     2101 caatcgccat ctcccgttcg ccggcagtcc attttcaggg actgctcacg cacgccggcc
     2161 actcatacaa cgcgcacgac gtcgatgaga tccgccgtat tgccgccgag gagacgtcgt
     2221 cgctgacgag gttccgcgcg ctgatcgaga acgaaggtct ggccggcgta gtgcgcagca
     2281 gcggctcgac accgacaacg tccgtcgtcg agacgttcac cgacaccgac gaggtgcgcc
     2341 ccggcaacta cgtcttcttc gacgccttcc agtccaccct cggctcctgc acacgcaaag
     2401 acgtcgccgt ctcagtgctc gccactgtcg tcggctccta tcccgagcgc ggcgaagcga
     2461 tcatcgacgc cggcgcgctg gcactatcga aagacacgag ccccgatcat ctcgatccaa
     2521 ccttcggcta cggcctcgtc tgcgatgtcg cgctccaccc gctcgatgcg cgcctcgtgg
     2581 ccctgtcgca ggagcacggc aagctcagga tgtccgcgcc cccaccggtc ggcacgcgcc
     2641 tccgcatcgt ccccaatcac tcctgcctca ccgccgcaat gtacgaccgc tatcacgtga
     2701 tcgatggcga tgcgatcgtc gatgaatggc gtccagtgcg gggttggtga aaaaccttat
     2761 ccgcagatga cgcagataga cgcagattga gacggggtca ggtctgaagt gtgaagtgac
     2821 ggaacacagc tttgccttcg aggaacgggc atcttccgtc acttcacact tcagacctga
     2881 ccccatctcc tatctgcgaa catctgcgac atctgcggat aagacgctaa cgcccttgca
     2941 gcaatcgcag cgccgcagtc ttcagcgcca tgctcgagcc ggcgcgtgcg atgctccgaa
     3001 tctccgactc cgggagcgtg ggcaggatac gtagcgcgaa ggcgggtggg gtcttggcgt
     3061 tgtgcatgag gccgaggcgg acgtcgagga cggacatcca ctgcgtggtc ttcatgatca
     3121 cgtcggcgat ctggtagttc aggaacgttg atttcgccag gcgcgccacc tcgtcgaccg
     3181 tgaggcgcgg gttgcggagc agcgagagca ggacgcgggg atcgttgtct tgaagaagca
     3241 gcgctcgctc gctgcggtcg gctttcacgg ccagcaggag cttctccatc tgcgacagcc
     3301 cgcggatgcg gtcccagacg ttctcccgtt tctcggtcgt ctcctcctgc tcgggcgcct
     3361 cttccgttgg cggttccgga tcgccgaggg gcttggccat gagacgggac agaagttcgt
     3421 cgctgttccc gtcgatcgag agcgcgatgc cgtcgggaag gagtgccacg acggtcgcgc
     3481 gtgcgaacga ttcgccggcc cacggtccgc gcagcgtcac caggagcgtg gcgttgagtg
     3541 cgaccgtctc ggccgtgact aaacgaagcc cgcccatcga gagattcgtg cggtgctctt
     3601 cgcgcagctc ctcctcactc tcgaattcgg cggtgaaggc ggcagaagcc gtgcgggtga
     3661 cgttcatttg cgtggaggcg aagggcacac tgattctacg cttggccgat acggtcacgg
     3721 ggacgctcga tagcgtgcgc gtcacgcagt cactcgtcgg cgccacgctg cgagtggtgg
     3781 gcgccacgct gcgattcgtg cgcgccacca gccaactggt gctcagccac ttgtttagac
     3841 cattcaccac aaaggcacaa aggacacaga gaacctttgt gctctttgtg tctctgtggt
     3901 gaatccttga aggtcacatt cactgccacg tcacgttcca ttcgcaatgc ggcgctcctt
     3961 tggcgtagca ctgcgtctgt tggacgcgca cgtccttcac accggtcagt tccgccatcc
     4021 gctcgatcca tcctgtgatc cgcgcgcagc cggcggtctc ggatgggaag tcgagcagct
     4081 tgatcgtcgc gccgcggtcg gtctggttct cgatgcgcat ctgcccgcgg ttgtagagcg
     4141 tgctccacat gctcgaggct ttgctgagca gcgcagccgg cgtgaggatc agggcgaaga
     4201 aacggtagat gcctttgacc gcgtccgacg ctccctcgcg gccggcgcgg cgggcgaact
     4261 gttccaccgt ttcgcccttc gccttggcgc cctcggccgt cagcgcattg agaatgtgaa
     4321 cagggatcgt ctcggtggcc agcacgatcc cgcccaagcg tgtggcgtac tcagcgggga
     4381 gcgtctgcaa cgcggtggcg cgctgctctg ccgagagctc ggactcgaga aacttctgca
     4441 ggctgcgaac cggcgagcct ttgacggtaa cgctctcatc tcgtgccagg gaagtcctcc
     4501 actcgctgcg agttcgaccg gatgcccctg agtataatcc acgcgcccgc gggcagcgga
     4561 aagcgttccg gcagcaacgg gaacctctgc cccagtccgc ggcgcaccgg aggcaacatg
     4621 gacgaacaga caggtggcgc ggcagaggcc agcgccgcta tcgcggaagc ggaatcgcac
     4681 aacgagccga agagcggcgc gaaggacagg ctggaagagc tttgcgtcaa cacgatccgc
     4741 ttcctcgcca tcgacggcgt cgagaaggcg aagtccggcc atcccggcgc gccgatgggc
     4801 atggccgaca tggcttacgt cctctggacc gatttcatgc gccacgatcc gaaggcgccg
     4861 tcgtggcagg atcgcgaccg cttcgtgctc tcggccggtc acgcatcgat gctgctttac
     4921 tcgctgctgc atctaaccgg ctatccggag atgaccatcg atcagttgaa aagcttccgg
     4981 cagtggggct cgaaaaccgc cggacaccca gagtatggcg acgcgcccgg catcgaagtc
     5041 acgaccggtc cgctcggcca gggattcgcg aacggcgtcg ggatgggcat cgcctcgaag
     5101 atgatggcgg cccgcttcaa caccgaagat ccattcaagc ctgtccagaa ctggatctac
     5161 gtcatctgct cggacggcga tctcgaagaa ggcatcagtt ccgaggccgc gtcgcttgct
     5221 ggtcacctcg
//
LOCUS       DATLVW010000779         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_242159, whole genome shotgun sequence.
ACCESSION   DATLVW010000779 DATLVW010000000
VERSION     DATLVW010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242159"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VNN25_22810"
     CDS             complement(<1..779)
                     /locus_tag="VNN25_22810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXH94426.1"
                     /translation="MPIPRDARKTRRRSNAVAATIHDVAARAGVSVATVSRVLNGIAV
                     VREETSRDVRAAAKFLRYVPNVAARSLSIRRSQTIGIVLPDVHGEFFSEVIRGIDLAA
                     RREGYHILVSGSHSDPSEMLEVVETMRGRVDGLMIMAPDVTLAPIEELRARELPVVLL
                     NAAAGTGDAITIDNFGGARAVMHHLHELGHTRIAFVCGPLHNADARERLRGYRHAMRG
                     LETAALRALEFQGAFTEESGFGAGQKIAATVPRPTAVFAAN"
     gene            967..2319
                     /locus_tag="VNN25_22815"
     CDS             967..2319
                     /locus_tag="VNN25_22815"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HXH94427.1"
                     /translation="MSDVRFPPGFVWGTATAAYQIEGASQDDGAGPSIWDRFSHTPGL
                     MTNGDTGDIACDHYHRYPEDIAIMRDLGVQGYRFSISWSRVLPEGTGRVNQKGLDFYR
                     RLVDALREAGIRPMATLYHWDLPAALDDRGGWLNRDVAEWFAEYASVAYKALDDTVEW
                     WATLNEPWVVTDGGYLHGALAPGHRNLFEAPIATHNLLRAHGRAVQVYRAEGKHQVGL
                     VVNLEPKYPFSQSEEDLAANRRADAYMNRQYLDPAFKGAYPAEMKEMFGAAWPDFPAA
                     DFDLIKQPLDFVGINYYLRAVTKADPTNPIERATRVRQQGAIYTETGWEVYPPALTAV
                     LLWFKEHYGPTPIYITENGAAFYDPPTAIDGTVNDPLRIHYLREHILSVHRAIEQGAD
                     VRGYFVWSLLDNLEWSHGYTKRFGIVHVDFATLKRTLKESAKFYREVVRTNGGAVGSP
                     "
BASE COUNT          452 a          751 c          744 g          421 t
ORIGIN      
        1 tcgttggcgg cgaagacggc ggtcggccgc ggcaccgtcg ccgcaatctt ctgccccgcc
       61 ccaaatcccg actcctcggt gaacgcgccc tggaactcca gcgcgcgtaa cgccgcagtc
      121 tccagaccgc gcatggcgtg ccggtagccg cgcaaccgtt cgcgcgcatc ggcgttgtgc
      181 agcggaccgc agacgaaggc gatgcgcgta tgtccgagct cgtgcagatg atgcatgacg
      241 gcgcgcgcgc cgccgaagtt gtcgatggtg atggcgtcgc cggttccggc cgcggcgttg
      301 agaagaacga ccggcaactc acgggcccgc agttcctcga tcggagcgag cgtgacatcg
      361 ggtgccatga tcatcagtcc gtcgacacgg ccgcgcatcg tctcgacgac ttcgagcatc
      421 tcggaaggat cactgtgcga accggaaacg aggatgtggt acccctcgcg ccgcgcggcc
      481 aggtcgatgc cgcgtatgac ttccgagaag aactcgccgt gaacgtcggg aagaacgatg
      541 ccgatggtct ggctgcggcg aatgctcaac gaccttgcgg caacgttggg aacgtagcgc
      601 aggaacttgg ccgcggcacg gacatcgcgc gacgtctcct cgcgcacgac ggcaatgcca
      661 ttcaggacgc gtgagacggt ggcgacggat acgccggcac gcgccgccac atcatgaatc
      721 gtcgccgcca ccgcgttgct gcggcgtctg gtttttcgcg cgtcccgtgg aatcggcatg
      781 agtagtggat tgcgattcgt cgatggatgg gtgctcgtcg atcagttggc tggcgggttg
      841 gggatcacgt ttgcgtccgg ttttttcgaa tcgcgggtca aggaaaagcg cgattcgtgt
      901 aaacgtttac atttttgcgg tacgctactc acaattcgcg tggctgtcaa tgaggagaat
      961 gaatcgatga gcgatgttcg ctttcctccc ggattcgtct ggggcaccgc aacggcggcg
     1021 tatcagatcg agggcgcatc gcaggacgac ggtgccggac ccagcatttg ggaccgattc
     1081 agccacactc ccggcctgat gacgaatggc gatacgggcg acatcgcctg cgatcactat
     1141 caccgttatc ccgaagacat cgccatcatg cgcgacctcg gcgtgcaggg ctaccgcttc
     1201 agcatttcct ggagccgcgt cctgcccgag ggtactggtc gcgtgaatca aaaaggattg
     1261 gacttctacc gccgcctggt cgatgcgtta cgcgaagccg gcatccgccc gatggccacg
     1321 ctctatcact gggatctgcc tgccgcgctc gacgatcgcg gcggctggct caaccgcgac
     1381 gtcgccgagt ggttcgccga gtacgcgtcg gttgcgtaca aagcgctcga cgatacggtg
     1441 gagtggtggg ccacgctcaa cgagccatgg gtcgtcaccg acggcggcta tctgcacggt
     1501 gcgctcgctc cgggacaccg gaacctgttc gaagcaccga tcgcgacgca caacctcttg
     1561 cgcgcgcatg gccgcgcggt gcaggtgtat cgcgccgaag gaaagcatca ggtcggcctg
     1621 gtagtgaatc tcgagccgaa gtatcccttc tcgcagagcg aagaagacct tgccgcgaat
     1681 cgccgcgccg atgcgtacat gaaccggcag tatctggatc cggcattcaa gggtgcctac
     1741 cccgcggaga tgaaggaaat gttcggcgcg gcctggcccg actttcccgc cgcagacttc
     1801 gacctcatca aacagccgct cgatttcgtt gggatcaact actacctgcg cgcggtgacg
     1861 aaggcagatc cgacgaatcc gatcgagcgc gccacgcgcg tccgccagca gggcgcgatc
     1921 tacaccgaga ccggctggga ggtttacccc cccgccctca ccgccgtcct gctctggttc
     1981 aaagagcatt acggaccgac tccgatttac atcaccgaga acggcgccgc gttctacgac
     2041 ccgccaaccg ccatcgacgg caccgtcaac gatccgctgc gcatccacta cctgcgcgag
     2101 cacatcctct ccgtccaccg cgccatcgaa cagggtgccg acgtccgcgg ctacttcgtc
     2161 tggtcgctcc tcgacaacct ggaatggagc cacggctaca cgaaacgctt cggcatcgtc
     2221 cacgtcgact tcgcgacgct caaacggacg ttgaaggaga gcgcgaagtt ctatcgagag
     2281 gttgtgagga cgaacggcgg ggcggtgggg tcgccgtaaa aagttcgtgc tcaagggctt
     2341 ttaccacaga gacacagagg acacagag
//
LOCUS       DATLVW010000780         5441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_443399, whole genome shotgun sequence.
ACCESSION   DATLVW010000780 DATLVW010000000
VERSION     DATLVW010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443399"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..783
                     /gene="coaD"
                     /locus_tag="VNN25_22820"
     CDS             295..783
                     /gene="coaD"
                     /locus_tag="VNN25_22820"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXH94428.1"
                     /translation="MRNAIYPGSFDPLTNGHLDIIRRGAQLFDRFLVAVLENEGKSPL
                     FSVAERKELIVRCTSDIPNVEVHSFSGLLVDFMRRVDASVVVRGIRAVSDYEYELQMA
                     LMNRELHPQSETIFMLPAVEYTYVSSRLVKEVFRLGGDVGRLVPPTVLASLKARLPVT
                     AG"
     gene            complement(876..1727)
                     /locus_tag="VNN25_22825"
     CDS             complement(876..1727)
                     /locus_tag="VNN25_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94429.1"
                     /translation="MWRITWRRFCRSKLALVALVYVGVVALVALSAPFLAASKSSPIP
                     FGPNEIDVANRLHPPDAQHRLGTDELGRDVLARMIHGARVSLTVGLIATAMSALVGSF
                     LGALAGFYGGAADWIVSRLIEIVLCFPFLFLVLAIVAFFGPSIWTIMLALAITNWTSE
                     ARYVRGEVMRLREMEFAHAARASGARDYRIIFRHLLPNALAPVLVSVSFGVAYAIVTE
                     SALSFLGLGVPLPTATWGSILSSAKEFVEYAWWLIVFPGLAIFMTVAAFNIIGDRFRD
                     ALDPRSD"
     gene            complement(1731..2714)
                     /locus_tag="VNN25_22830"
     CDS             complement(1731..2714)
                     /locus_tag="VNN25_22830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94430.1"
                     /translation="MLTYVVRRLLYGVLTFFGITIAIFALVHSVPGDPLSFYIGSHGM
                     GALSHAALDEIRHEHHLDLPIVQQYGWWLRGIVTLDFGTSFIDHRPVTELIAEKLPNT
                     FILNFVAFLVAALIGVPIGLWSATRSGRTSERASGVAFFLLYSLPSFWVALLLMDLFS
                     VRFHVLPLFGMTSDNYLDLGLASKLADRALHLILPVVTLAYAQMAIYARFSKSALTEV
                     IRQDFITTARAKGATSAAVLWHHALRNALIPLITLLGLTLPYLVSGSVIVEEIFQWDG
                     IGRLYFDAISQRDYPTVLGLSVATAVATLLASLLADILYAVADPRIRLEGK"
     gene            complement(2953..4608)
                     /locus_tag="VNN25_22835"
     CDS             complement(2953..4608)
                     /locus_tag="VNN25_22835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXH94431.1"
                     /translation="MRSLRTAFALSLFLSLFLVAGCSKEQPLPQQPVIEDDSTPVDGG
                     TVIRRLQADINTLNPLLIASKYDHYIAAYLFTPLLQLDANLLPTTAGSLTDKYEVSPD
                     GKLYTFHLNPKATFSDGTPVHASDAVFSLKKIVDPKSESAEFAGWFEKLDAAGTKAVD
                     DHTLVVAFTEARAPQLVSFNGVRVIPEHVYAKGDFKSAFGMSAVGSGPYRLVRRIPGK
                     EILLERRSDYWGVPPHIQNVLFKIIGDDNTAWNALKRGDIDETIIGSDTWLNESRRPE
                     LQKTINFRRFYTLNYNYIGWSEHDPLLADKRVRRALAMCVDMQSVIDGLYHGTARAMN
                     GPFTPDQWSYNPEVPVIQFNPEGARQILNSMGWQDTDGDGILDKDHKPFAVEIIIAGG
                     SPTSVAFTQLYQAELKKVGIDLKISPLDGGAFIQRLVSGNFQAAYASWDLDPDPDPNA
                     IYHSSQFPPGGQNFVFYKNPAADALIDQGRHELDHTKRVAIYRQLHALLADDQPYTWT
                     VQVSLKWALTKRIKNAKESKGWGFFFWYPGELDWWIPKSQQGK"
     gene            4758..>5441
                     /locus_tag="VNN25_22840"
     CDS             4758..>5441
                     /locus_tag="VNN25_22840"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH94432.1"
                     /translation="MTTTLPVCDERSLSQLALGLTGSEILRISAEIRALSEAGRDVCN
                     LTVGDFSPKEFRIPRVLETLISEAFTAGETNYPPSDGTKELRQAVIRYYNDVLGLEYP
                     LESTIIAGGARPIIFAAYSAIVDPGDRVIFPIPSWNNNHYTYLMRGQAVEITVGPETN
                     FLPTADLIRPHVRDARMICICTPANPTGTVMARDEVERIARLVVEENERRAASGQRAL
                     LLLWDQIYWM"
BASE COUNT         1107 a         1638 c         1686 g         1010 t
ORIGIN      
        1 ctccagccgc tgtcgcatca ctcacaccct cccgaatctg tttcactcga attggtcatc
       61 atcttagttt cggattgggc gatcgtcaag aggttgacta ttggtttgtg gggagtgggg
      121 tgtggggtgt gggaggcaaa tcaagctgca ggaaggtggc gtgtcccatt caacacgcaa
      181 cgatcaaccc cgaacattca accttccggt tgtcaccgag gcctccacga aagctcgcag
      241 cactcccaca ccccactccc cacaccccac ccccctcctg taccatcttc cccaatgcgc
      301 aacgccatct acccgggctc gttcgatccg ttgaccaacg gccatctcga catcatccgc
      361 cgcggggcgc agctcttcga tcgtttcctg gtcgcggtgc tcgagaatga agggaagtct
      421 cccctcttct ccgtcgccga gcggaaggag ctgatcgtgc gctgcacttc ggatatcccg
      481 aacgtcgagg tgcactcctt ctcgggactc ctggtcgatt tcatgcgccg cgtcgatgcc
      541 agcgtcgtcg ttcgcgggat ccgcgcggtt tcggactacg agtacgagtt gcagatggcg
      601 ctgatgaatc gtgagcttca tccgcagtcg gagacgatct tcatgctccc cgcggtcgag
      661 tacacctacg tctcctcgcg cctagtcaaa gaagtcttcc gcctcggcgg ggacgtcggc
      721 cggctcgtgc cgccgacggt tctcgcgtcg ctcaaggcac gcttgccggt gaccgcggga
      781 taaaaccagg caccacagag acacagagat cacagagttc ttctaagtgt ttctctgtga
      841 tctctgtgtc tctgtggtgg tcggttctgt tttcgtcaat cactccgcgg atcaagcgca
      901 tcacggaatc ggtcgccgat gatattgaag gcggcgacgg tcatgaaaat cgccaggccg
      961 gggaagacga tcaaccacca agcgtactcg acgaactcct tcgcggagga gaggatgctg
     1021 ccccatgtcg cggtcggaag cggaacgccg agaccgagga atgagagggc ggactcagtg
     1081 acgattgcgt aggcaacacc gaagctcacg gagacgagaa cgggggcgag cgcattcgga
     1141 agcaggtggc ggaagatgat gcgatagtcg cgcgcaccgc tcgcgcgcgc cgcatgcgcg
     1201 aactccatct ccctaaggcg catcacctcg ccgcggacgt aacgcgcttc gctcgtccag
     1261 ttggtgatgg ccagagcgag catgatcgtc cagatcgagg gcccgaaaaa cgccacgatg
     1321 gccaggacga gaaagagaaa cgggaagcag agaacgatct cgatcaggcg cgacacgatc
     1381 cagtcagccg caccgccgta gaagcctgca agcgcgccga ggaacgagcc gacgagcgcg
     1441 gacatggcgg tggcgatgag accgacggtc agcgaaacac gggcgccgtg gatcatgcga
     1501 gccagcacat cgcgtccaag ttcatcggtg ccgaggcgat gctgcgcgtc gggagggtga
     1561 agacgattgg cgacatcaat ctcattcggt ccgaacggaa tcggcgacga cttggaggca
     1621 gctagaaatg gcgcactcag agcgaccagc gccacgacgc cgacgtacac gagcgcgacg
     1681 agtgccagtt tgctgcggca gaagcgccgc catgtgatgc gccacatgcg ctatttcccc
     1741 tccaggcgga tgcgcggatc ggccacggcg tagaggatgt cggccagcag actggccaga
     1801 agcgtggcta ccgcggtggc gacggagagt ccgaggacgg tcggatagtc gcgctgggag
     1861 atcgcgtcga agtagaggcg tccgatcccg tcccactgaa agatctcttc gacgatgacg
     1921 ctgccggaaa cgagatacgg cagcgtgagc ccgagcagcg tgatcagggg aatgagcgcg
     1981 ttgcggagcg cgtgatgcca gagcacggcg gccgaggtag cgcccttggc gcgtgcggtc
     2041 gtgatgaagt cctggcggat cacttcggtc agcgccgact ttgagaagcg cgcatagatc
     2101 gccatctggg cgtatgcgag cgtgacgacc ggcaggatga gatgcagtgc gcggtccgca
     2161 agcttcgacg ccagacccag gtcgaggtaa ttgtccgagg tcattccgaa aagcggcagg
     2221 acgtggaacc ggacggagaa taggtccatc agcaggagcg cgacccaaaa gctcggcagc
     2281 gaatagagga gaaagaacgc aactcccgag gcgcgctcgg aggtccgtcc ggaacgcgtt
     2341 gcgctccaga gtccgatcgg cacaccgatc aacgccgcca cgagaaaggc gacgaaattc
     2401 aggatgaatg tgttcggcag cttctcggcg atcagctcag tgacaggacg gtgatcgatg
     2461 aaggacgtgc cgaagtcgag cgtgacgatg ccgcgtagcc accacccgta ttgctgcacg
     2521 atcggaaggt cgaggtgatg ctcatgccgg atttcatcga gcgccgcgtg ggagagcgcg
     2581 cccattccgt gcgacccgat atagaacgag agtggatcgc ccggcacgct gtgaacgagc
     2641 gcgaagatgg cgatggtgat gccgaagaac gtcagaacgc catacagaag gcggcggacg
     2701 acgtaggtga gcatgcggcg agtttagacg gtttcccgcg gagccgtgga atcgggtcgc
     2761 gggtcaattt ggacggctta tcgtcgtcgc ccaagcaaag gcaaccacag agacacagag
     2821 aacacagagg tgttctctgt gacctctgtg cctctgtggt gagcgggttt tcaaattgaa
     2881 cccactaccc ggagtcgcgg aaattcgaaa cctcgcgatc tccgcgtctc cgcgtgaagc
     2941 aacaaggcga gcctacttcc cctgctgcga cttcggaatc caccaatcga gctcgcccgg
     3001 gtaccagaag aagaagcccc agcccttgct ctccttcgcg ttcttgatcc gcttcgtgag
     3061 cgcccatttc agcgacacct gcaccgtcca cgtgtacggc tggtcgtcgg cgagcagagc
     3121 atggagctgg cgatagatgg caacgcgttt cgtgtgatcc agctcgtggc gcccttgatc
     3181 gatgagcgcg tcggccgcag ggttcttata gaaaacaaaa ttctggccgc cgggcgggaa
     3241 ctgtgacgag tgataaatcg cgttgggatc agggtccgga tcaagatccc acgacgcgta
     3301 cgcggcttgg aagttgccgc tgaccaggcg ctggatgaaa gcgcccccgt cgagcggcga
     3361 gatcttcaga tcgatgccca cttttttcag ctcggcttga tagagctgtg tgaaggcaac
     3421 cgatgtcggg cttccgccgg caatgatgat ctccacggcg aagggcttat ggtccttgtc
     3481 gaggatccca tcgccatccg tatcctgcca tcccatcgag ttgaggattt gccgcgcccc
     3541 ctcggggttg aactggatca ccggtacttc cgggttgtac gaccactggt cgggtgtgaa
     3601 cgggccgttc atcgctcgtg ccgtgccgtg atagagaccg tcgatcaccg actgcatgtc
     3661 gacgcacatc gccagggcgc gccggacacg cttgtccgcg agtaacgggt cgtgctcgct
     3721 ccagccgatg tagttgtagt tgagtgtgta gaaacgccgg aagttgatcg tcttctggag
     3781 ctcgggccgg cggctctcgt tcaaccaggt atcggagccg atgatggtct cgtcgatgtc
     3841 cccccgtttc agtgcgttcc atgccgtgtt gtcgtcgccg atgattttga agaggacgtt
     3901 ctggatgtgc ggtgggacac cccagtagtc gctgcggcgc tccaggagaa tctctttgcc
     3961 gggtatacgc cggacgagcc ggtaaggccc cgagcccacc gcgctcatgc cgaaagcgga
     4021 cttgaaatcc cctttcgcgt acacatgctc gggaatcacg cgtacgccgt tgaaggagac
     4081 gagctgcggt gcgcgcgctt ctgtgaaagc cacaacaagt gtgtggtcat ccacggcttt
     4141 ggtgccggct gcatccagct tctcgaacca gccggcgaac tccgcggact ctgacttcgg
     4201 atcgacgatc tttttcaacg agaagacggc gtcgctggca tgtacgggcg tgccgtcgga
     4261 aaaggttgcc ttcgggttga gatggaacgt gtaaagcttc ccgtccggag atacctcgta
     4321 cttgtcggtc agcgaaccgg cggtcgtggg aagcagattg gcgtcgagct gtagcagcgg
     4381 cgtgaacagg tacgctgcga tgtagtgatc gtacttgctc gcgatcagca gcggattgag
     4441 cgtgttgatg tccgcctgca accgccggat gaccgtcccg ccgtccaccg gtgtcgagtc
     4501 atcttcgatg accggctgct gcggcaacgg ttgctctttc gagcagccgg caacaagaaa
     4561 caggctgagg aacaggctga gagcgaaggc agtacgaagg cttctcacac gacgattata
     4621 gtggtcaccg gactgagccg aggatggtct tcgccggtgt ccctctttga gatgctgatt
     4681 cgaccgcgga gattctacgt gtcgtatcct ctcagaccgc tgtttctgcc catcgactga
     4741 tctacaatcc accaccgatg acgacaacat tgcccgtctg cgacgaacgc agcctctcgc
     4801 agctcgccct cggccttaca ggttccgaga tcctccgcat ctcggccgag atccgcgcgc
     4861 tcagcgaagc cggccgcgac gtctgcaacc tcaccgttgg cgacttctcg ccgaaagagt
     4921 tccgcatccc gcgtgtgctc gagacactga tatccgaggc gttcacggcc ggcgagacga
     4981 actatccgcc gtctgatggc accaaggaac tacgccaggc ggtcatccgc tactacaacg
     5041 acgtactcgg cctcgagtac ccactcgaat cgacgatcat cgccggcggc gccaggccca
     5101 tcatcttcgc tgcgtactcc gccatcgtcg acccgggcga cagagtcatc ttccccatcc
     5161 cttcctggaa caacaaccac tacacctacc tgatgcgcgg ccaggcggtc gagatcaccg
     5221 tagggccgga gaccaacttc ctcccgaccg ccgacctcat ccgcccgcac gttcgcgacg
     5281 cgcgcatgat ctgcatctgt acgccggcca atccgaccgg aacggtcatg gcgcgcgatg
     5341 aagtggaacg gatcgcccgt ctcgtcgtcg aagagaacga acgccgcgcc gcctcggggc
     5401 agcgagcact gctccttctc tgggatcaga tctactggat g
//
LOCUS       DATLVW010000781         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_158734, whole genome shotgun sequence.
ACCESSION   DATLVW010000781 DATLVW010000000
VERSION     DATLVW010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158734"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VNN25_22845"
     CDS             complement(<1..399)
                     /locus_tag="VNN25_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94433.1"
                     /translation="MKYRFIAVLLAALTWDRLFACSPWIVVPHVLDPEEQKVDRTPPV
                     VPELRVLKVVRGHDPITLHGDSYTFDTCADKAVVLVGFTVPPSDDRTPVDKLGVSWRI
                     LRGTPPAGLREPAVFSQAQVVLIDWTERNNK"
     gene            complement(508..861)
                     /locus_tag="VNN25_22850"
     CDS             complement(508..861)
                     /locus_tag="VNN25_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94434.1"
                     /translation="MDIDTLISVASDNSLRPSEIEQTAQELGCTIHELCDRFAKELAK
                     RYLDEQIAWEPADAAINKLYAIAYGSADFGLGEFAWSVYLAFDEGEYIHAGGDPEPDG
                     EPRTRKILSSLIAGT"
     gene            complement(1000..1533)
                     /locus_tag="VNN25_22855"
     CDS             complement(1000..1533)
                     /locus_tag="VNN25_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94435.1"
                     /translation="MPETYDAAVDLAGVLVDASYACAESFRTFIDESFEESESSEKHE
                     QLVSEFFFAFLHMLDRTMLAERGVDFRDQNIDPLATLALRERASRQHPALNANVMETF
                     LVLLGRDANDAHSAYSNCSSDPDQPFTGNGVMNRLAPIVCRYLDCPNNPELHRQAISE
                     FAHFSEIVNLRNVVTVL"
     gene            complement(2244..2531)
                     /locus_tag="VNN25_22860"
     CDS             complement(2244..2531)
                     /locus_tag="VNN25_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94436.1"
                     /translation="MPFPDRHPELALYLRLGTVIILLGAFWSVVGALAGSNDGQQTGE
                     AGLAPWQLAWWEQFGWRYGVALIVLGASILVFRAVWKRSLLFGDRIRHEYE"
     gene            complement(2641..>2884)
                     /locus_tag="VNN25_22865"
     CDS             complement(2641..>2884)
                     /locus_tag="VNN25_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94437.1"
                     /translation="LYFSQGPRLHDPKRILLGSGKQTRFIQVEAASQLAHPDVEAFIA
                     DTIEQARIPLPLEGKGSLIIKSSAAAKKRPRRRPAK"
BASE COUNT          607 a          851 c          861 g          565 t
ORIGIN      
        1 cttgttgttt ctctcggtcc agtcgattaa gacgacttgc gcttgactga acaccgccgg
       61 ttctctcaag cctgcgggcg gtgttcctcg aagaatgcgc catgacacac ccagtttgtc
      121 gacgggcgtg cggtcgtcgg acggcggcac ggtgaagcct acgagaacta cggccttgtc
      181 cgcgcaggta tcgaacgtat acgagtcgcc atgtagagtg atcggatcat gaccgcgcac
      241 gacttttaga actcgcaact ccggcactac cggcggggtg cggtcgacct tctgctcctc
      301 gggatcgagc acatgtggca cgacaatcca cggcgaacaa gcgaacaacc tgtcccaagt
      361 aagtgctgcg agaagtaccg ctatgaaccg atatttcatt cgtgtgtcgc ccaacggacc
      421 tgcgcttcag cggcgtggcc cgcgacctac atcgtaacgt acttgcgaca gggcgactca
      481 gcgggccacg tccgcggcaa gcgcgagtta ggtgccggcg attagcgagc tcaaaatctt
      541 gcgagttctc ggttcaccgt caggctccgg atcgccgccc gcatgaatgt actcgccctc
      601 atcgaaagcc aggtacacac tccatgcgaa ttcacccagg ccgaaatcgg cgctgccata
      661 cgcgatggcg tacagtttgt ttatggcggc atcagcgggt tcccaggcga tctgctcgtc
      721 gagatatcgt ttcgcgagtt ctttcgcgaa cctgtcgcac agctcgtgaa tggtgcagcc
      781 aagctcctga gccgtctgtt cgatttcgct cggcctcagg gagttgtccg acgcaactga
      841 gatgagggtg tcgatgtcca tgaattcggc gtctaacgga tcacgcttca gccgcgtggc
      901 ccgcgaagcg aaatagtaac gcgctcgagg cgccgcaagg cagcgagcca cggcgcctgc
      961 aagcgagggt cagcctgtgg acggaagatg ctcgatagct cacagcacgg tcaccacatt
     1021 tctcaagttc acgatctcgc tgaaatgagc gaattcggag atcgcctgtc gatgtagttc
     1081 cgggttatta ggacagtcga ggtatctaca gacgatcggc gcgagcctgt tcatgacccc
     1141 gttgcctgtg aacggctggt ctgggtcact cgaacagttc gaatatgccg agtgagcgtc
     1201 gtttgcgtct cgaccaagta gtacgaggaa ggtctccatg acattcgcat tcagggctgg
     1261 atgttggcgg ctggctcgtt cgcgaagagc gagagttgcg agcgggtcga tgttctggtc
     1321 gcgaaaatca acgccgcgtt ctgcaagcat cgttcgatcg agcatatgca agaaggcgaa
     1381 aaagaactcc gaaacgagtt gttcgtgctt ctcggatgac tcgctttctt caaaagattc
     1441 gtcgataaag gtacgaaagg attctgcgca cgcgtatgag gcgtcaacga ggacacccgc
     1501 gaggtccacg gcggcatcgt aagtttctgg catgacgcgc gggattgttg cttccgcagg
     1561 ctaacggccc gaagctcacc ccggcttgga gccgcacgtg gacgtgtaca gcactcgtga
     1621 cgaagcaagt tgcggctcca agtccggtgg agcgtcgagt tggccagcag aggctaatac
     1681 cagcactcga agtccactgc gcgccaaccc gttcgatccc tcttcagggt gatccacgtg
     1741 ttcgaaggca acgatgagtg gccgcggaag acagcaaaaa acagatgcgt tgtgctggaa
     1801 acatccttgc gatcgcgcag ggaccaagtt cgaacacgat acttgcgttc acgttcgcag
     1861 aggtcgctct gccgctcgcg tcctacgaat gcgagcgcgg cctcacagcg accggagcga
     1921 agctcgccaa gcacgtggct ggcgacccgt tccgcggacc gatctctggc cgggttgaga
     1981 accactagca atccatctcc cgggtcaacc gccgagggat ctttcacccg cagcagcgtc
     2041 ggcggcgcga atagtaggat gaccacgaga accgcgatga cggcgagcgt gatgccgagg
     2101 ctgcggagag gtgacatcgt gctggctaac gttttgccgc tcaccagcgc cgtgcttcag
     2161 gcggcaagag cgacacccaa gaatgatacg ccgagcaagg ccacgaacgg cgcggcgtcc
     2221 ggtgcagcgg ccgagttggg cgtctattcg tactcgtgcc ggatgcggtc accgaagagc
     2281 agcgatcgtt tccagacggc acgaaagacc agaatggatg caccgaggac aataagcgct
     2341 acaccatagc gccagccgaa ctgttcccac catgcgagtt gccatggagc cagacccgct
     2401 tcaccggttt gctggccgtc attgctaccc gcaagcgcgc cgacaactga ccaaaaggcg
     2461 ccgagaagga tgatcaccgt tccaaggcgc aagtagagcg ccagttctgg atgccgatcg
     2521 gggaatggca tgttcgttag tcgcccaacg ttttgcctct ctccggcgcg gtgcttcgca
     2581 cagcgagagt gacacccgat gatgatacgc cgaccaccgc agcgaacggc acggcgtccg
     2641 tcactttgcc ggccttcggc gtggccgctt cttcgcagca gccgacgact tgatgatgag
     2701 actgcccttc ccctcgagtg gcaacggaat cctcgcctgt tcgatcgtat cggcgatgaa
     2761 cgcttccacg tccggatgtg ccaattgact ggccgcttcc acttgtataa agcgtgtctg
     2821 ctttcccgac cccagcaaga tccgcttcgg gtcgtgcaac cgcggtccct gactgaagta
     2881 caga
//
LOCUS       DATLVW010000782         3465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_202707, whole genome shotgun sequence.
ACCESSION   DATLVW010000782 DATLVW010000000
VERSION     DATLVW010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202707"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VNN25_22870"
     CDS             <1..672
                     /locus_tag="VNN25_22870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94438.1"
                     /translation="FEGSMNEAPVWSVDGSEIIYASRDTPSERAAIYRRRADGNGPRQ
                     LVARLDGDADPGAVSADSRWVAATLWNRPGGNPQIWAVPLVPGEKAHPLLAGEPGTHD
                     PRISPDGRWLAYTSSESGRDEIYVVPFAGGGGKWQVTTVGGHSSRWSPDGAHLDYLTS
                     DNILTEVDVKSGGGFEIGAARALFRLDVNPVSSSDYDLTADGRVIANGGEADPTPLTL
                     VANWR"
     gene            complement(732..1244)
                     /locus_tag="VNN25_22875"
     CDS             complement(732..1244)
                     /locus_tag="VNN25_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94439.1"
                     /translation="MGAADLVTTEMALQLLNTVGLGLVGTGLYGVYKSLIQRSELLAK
                     SVNELEKAHDAVVKTMDKRSHEIDSRFDDEKKFRGLYLSLLEDAEVHRSKIKEWKEDE
                     LHALQARLELVAARLEQLEHDNRALQEKYYELESQLGTQVARNRFLEKQNKEFLVELG
                     KSGPTIRGLA"
     gene            1388..>3465
                     /locus_tag="VNN25_22880"
     CDS             1388..>3465
                     /locus_tag="VNN25_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HXH94440.1"
                     /translation="MRKSFLAFLVLLLALPLVADEGMWLFNRTPNAIFKQRYNFVPTQ
                     AWLDHLQKSSIRFNSGGSGSFVSADGLALTNHHVGLDCLAKISTKGHDYVTTGYYAKT
                     QADEVKCVDLELNVLQSIEDVTARVNASVTPGMTTAAAQAARRGVMSAIEQESVQKTG
                     LRSDVVTLFQGGEYHLYRYKKYTDVRLVFAPEVGIAFFGGDPDNFEYPRYDLDVCFFR
                     AYENGKPVHPADYLKWSASGAKDGDLVFVSGHPGSTSRLNTLTHLQFFRDVAYPFTLT
                     LLRRREVLLTNYAQRSIENDRRAHDDLFGVKNSRKAYLGRIAGLQDPAVLKAKADSEA
                     AMKQKVAADPQLSAAYGDAWQNVDAAIATYRPIYTEFRFVEGGLAFNSALFQHAKTLL
                     RLADESAKPNGERLREYRESNMPSLKQGLYSEAPIYEDLETVTLADSLSNWLEVAPND
                     PLVQQVLAGKSPQERASELVRATKLRDVAARKALGEGGKAAVDASTDPMIQVARMVDG
                     RARELRKQYDDNIAEVLRQSYAKIANAQFKTSGGDTYPDATFTLRLSYGTVKGYTENG
                     KTIPSSTMIGDTFEHAAEHGNEGEFKLPKSWMEKKTLLDDDKTPYNFVMTADIIGGNS
                     GSPVVNRENEFVGIIFDGNIQSLVLDFAYSDVQARAVSVDSRGIVEALKKVYGADALA
                     SELNTGKRGN"
BASE COUNT          681 a         1091 c         1021 g          672 t
ORIGIN      
        1 ttcgaaggct cgatgaacga ggctcctgtc tggagcgtcg atggcagcga gatcatctac
       61 gcctcccgcg atacgccctc ggaacgggcc gccatctatc gccgccgcgc ggatggcaac
      121 ggaccgcgcc agctcgtggc ccgtctcgac ggagatgccg accccggcgc cgtctctgcc
      181 gatagccgtt gggtcgcggc gacgttatgg aacagacctg gaggcaatcc ccagatctgg
      241 gccgtacctc tcgtacctgg cgagaaggcc catccgctcc tcgccgggga gcccggtacg
      301 cacgaccctc gcatttcccc ggatggccgc tggctcgcct acacatcgag cgaaagcggc
      361 cgtgacgaga tctacgtcgt cccgttcgcc gggggcggcg gaaagtggca ggtcacgacg
      421 gtcggcggcc actcatcacg ctggtctccc gacggcgcgc acctcgacta cctgaccagc
      481 gacaacatcc tcaccgaggt cgatgtgaaa agcggaggag gcttcgagat cggcgcggcg
      541 cgcgcgctgt tccgtctgga cgtgaatccg gtctcctcat cggactacga cctgaccgcc
      601 gatggccgcg tgatcgccaa cggcggcgaa gcggatccga cgccgttgac gcttgtcgcg
      661 aactggcggt gaaggacgcg gttatcccgt tctttctcgc tgccgcgacc tatgcctact
      721 ctctgccagt gttacgcgag tcctctgatt gtcggaccag actttcctag ctcaaccaaa
      781 aactctttgt tttgtttctc gaggaatcgg tttcgcgcga cttgtgtccc tagctgcgat
      841 tcgagttcgt agtatttttc ttggagcgcg cgattatcgt gctcaagttg ttcaagtctc
      901 gccgcaacta gctcaagtcg cgcttggagg gcatggagct cgtcctcttt ccattccttg
      961 atcttgctcc gatgaacctc agcgtcttcg agtaaggata ggtaaaggcc acgaaacttc
     1021 ttttcgtcgt cgaatcttga gtcgatctcg tgtgatcgct tatccatcgt tttcacaaca
     1081 gcatcgtggg ctttctcaag ctcgtttacg ctcttggcga gtagttcgct tcgctgaatg
     1141 agcgatttgt agacgccgta cagacccgtg ccaaccagcc ctagcccaac cgtgttcagt
     1201 agttgaagtg ccatctccgt cgtgaccaag tctgctgcgc ccatggccga tgtcctcctg
     1261 tgttaggagt gacgtgctaa aggctttccc tacccaacac ctctgaggcg agaaatggtc
     1321 gatcctgcag cctccgcttc cgtttgtgca tagaatcccg ctcgcaactg aactggaggc
     1381 aacattgatg cgcaagtctt ttctcgcatt cctcgtgctg ctcctcgcgc ttccgctggt
     1441 cgcggacgag gggatgtggc ttttcaaccg aactcccaac gcaatcttca agcagcgtta
     1501 caacttcgtg ccgacgcagg cgtggctcga tcatctgcag aaatcgtcga tccgattcaa
     1561 cagcggcggc tccggctcgt tcgtctcggc tgatggcctg gcgctgacga atcatcacgt
     1621 cggtctggac tgcctggcca agatctccac caaaggccac gactacgtca cgaccggcta
     1681 ctacgcgaag acgcaggctg acgaagtgaa gtgcgtcgac ctcgaactga acgttcttca
     1741 gtcgatcgag gacgtaactg cgcgtgtcaa cgcctcggtc acacccggca tgaccactgc
     1801 ggctgcacag gcagcgcggc gcggcgtgat gagcgcgatc gagcaggagt cggtgcagaa
     1861 aacgggactt cgcagcgatg tggtcacgct cttccagggc ggcgaatacc acctctatcg
     1921 ctacaagaag tacaccgacg tccggctcgt cttcgccccc gaagtcggca tcgcattctt
     1981 tggcggcgat cccgacaatt tcgagtatcc ccgctacgac ctggacgtct gcttcttccg
     2041 cgcgtacgaa aacggcaagc cggtgcaccc ggcggattat ctgaagtgga gcgcgagcgg
     2101 cgccaaggat ggcgatctcg ttttcgtctc cggccatcct ggctcgacca gcaggctgaa
     2161 cacgctgacg cacttgcagt tcttccgcga tgtcgcctac ccgttcacgc tgacgctcct
     2221 ccgccgacgc gaggtgctgc tcacgaacta cgcgcagcgc agcatcgaaa acgaccgccg
     2281 cgcgcacgat gatctcttcg gtgtcaagaa ctcacgcaag gcctacctcg ggcgcatcgc
     2341 cggcttgcag gatcctgccg tgttgaaagc caaagctgat tccgaggctg cgatgaagca
     2401 gaaagtggcg gcggatccgc agctcagcgc cgcctacggc gacgcctggc agaacgtcga
     2461 tgcggcgatc gcaacgtatc gtccgatcta cacggagttc cgattcgtag agggaggact
     2521 tgccttcaac agtgcgctgt tccagcacgc gaagacgctg ctccgtctcg cggacgaatc
     2581 ggcgaaaccc aacggcgaac gcctgcgcga gtatcgcgaa tcgaacatgc cctcgctcaa
     2641 gcagggcctc tattcggagg cgccgatcta cgaggatctg gagacggtga cactggccga
     2701 ctcgctttcg aactggctcg aagtggcgcc gaacgatccg ctcgtgcagc aggtgctcgc
     2761 cggcaaatcg ccgcaggagc gtgcctcgga gctcgtgcgc gccacgaagc ttcgcgacgt
     2821 tgccgcacgc aaagcgctcg gcgaaggcgg caaggctgcc gtcgacgcat cgaccgatcc
     2881 gatgatccag gtcgcacgca tggtcgacgg gcgcgcccgc gagctgcgca agcagtacga
     2941 cgacaacatc gccgaggtat tgcggcagtc gtacgcgaag atcgcaaacg cgcaattcaa
     3001 gaccagcggc ggcgacacgt accctgacgc caccttcacg ctgcgactct cgtacggcac
     3061 ggtgaaggga tacacggaga acggcaagac gatcccgtcg tcgacgatga tcggcgacac
     3121 cttcgagcac gccgccgagc acggaaacga aggcgagttc aagctgccga agtcgtggat
     3181 ggagaaaaag actctgctcg acgacgacaa gacgccctac aacttcgtga tgaccgcgga
     3241 catcatcggc ggcaactccg gaagcccagt cgtcaatcgt gagaacgagt tcgtcggcat
     3301 catcttcgac ggcaacatcc agtcgctcgt gctcgatttc gcctacagtg atgtgcaggc
     3361 gcgtgccgtg tccgtggatt cgcgtgggat cgtcgaggcg ctgaaaaaag tttacggcgc
     3421 cgacgcgctg gcgagtgaat tgaacacggg aaagcgcggg aactg
//
LOCUS       DATLVW010000783         5941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_321747, whole genome shotgun sequence.
ACCESSION   DATLVW010000783 DATLVW010000000
VERSION     DATLVW010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321747"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..597
                     /locus_tag="VNN25_22885"
     CDS             187..597
                     /locus_tag="VNN25_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94441.1"
                     /translation="MCLLLLVPAAAFAGFGDDFYEGLYARGMAHFHASEYEAAFNDLH
                     LAAFGLVDQLERFETAEAYAAISANRIGNEALTRSALVGISKADGVQPHFRSIAIPES
                     ERAELERAAALLLTNEERAVLRSAGPTQRPAAFR"
     gene            801..1790
                     /locus_tag="VNN25_22890"
     CDS             801..1790
                     /locus_tag="VNN25_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94442.1"
                     /translation="MAPSVLLAGGVDEFYERLYTRGMADFRTADYQLAYTELHKAAFG
                     FVEEIEKFETAEVYAAIAANRLGHSGEARDALLRIAAAEEVQPHLRSMTIPDDLRAEL
                     LRAAATLLTKKEAAAFGISEAMQDAAAKEHPRVEVPTPSVGPNVAETAPRGAGDANAP
                     DGAPEDPPAASSSTPAADPPPAASREEPAAQPGTRQSIAPMVPPTQQTAKKTTDQRLD
                     EAQQAIDSGDPDNARSIYDALLQEPQLPHAPAMRLAEGLYRVRDFAGASRAFHGVREI
                     GKGEERYHYYYAVALFETRHYRDACRELDLALPFIEVTEDVAGYREKIYKATK"
     gene            2002..2238
                     /locus_tag="VNN25_22895"
     CDS             2002..2238
                     /locus_tag="VNN25_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94443.1"
                     /translation="MAGKSKQDDEMRDHYDFSGGVRGKYAARYAEGTNVVVLAPDVAD
                     MFPDSIAVNEALRTFVRMTTRTVRAKAAPKKRAG"
     gene            complement(2938..>5941)
                     /locus_tag="VNN25_22900"
     CDS             complement(2938..>5941)
                     /locus_tag="VNN25_22900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94444.1"
                     /translation="DGAGYVWAGTPDSGLLRSVVPFDPAALKPGNVSRFFAPAWSHAT
                     GARTDSVRTLLWAAGKLWSGTANGLEILDTNPLRIVASLPGSSLGGSLVAGLMSSPNG
                     KSVWVSQNAGLVEVDTSDFHVRTRVSKADGLIDDEAWAYDPLAVGDDGRVYFATPRGL
                     SIFNPALREPNAEPPIVRFRDITFREDRRGNNEVSIAYAALTFSDESRVVYRTRLFGY
                     DNDWAAARSDAKIRYTNLPAYLFPKSYRFAVMARSSDGVWSLPELYTFSVMPPWWLRW
                     WAMIAYAAAIVALASVANRLRVRRLRRRNRELEDIVSARTHELEALDRMVEIINRELV
                     LENVFRALLDQGTKLFPQTEAAAFLQFDHDRGSTEVVAELGYDQESFSDVRLTPEEAT
                     RRYSERAELLEEGVYLIKGDVFHELAGSEQTKHFRVPKSMLAMEVSLGGRIEGFLIFD
                     NFSDPNAFSRSDLRKLARVREHAISAISKARILRELQLKNREAEEANQAKSRFLANMS
                     HELRTPMNAIIGFSEILIDRLEAQIDAKSLTFIRSILSSGRHLLEIINDILDLSKVEA
                     GRMELLPETFPVRSAIESICQIMRGMSTRKAISFAVEIDPGVTAIETDQSKFKQILYN
                     LLSNAVKFSPTESVVKIHARRVEATEERAESISIDVTDPGIGIAPENLTLIFEEFRQV
                     DGTLNRQYGGTGLGLSLVRKFVELQGGTLTVASEIGKGSTFTFTLPIHFAGTTIPSPI
                     INVDGTVIPPGERVLVVEDEDVAYSTLATYLQSAAYVPIRARNGDEAMRLARSIKPVA
                     VTLDIVLPGAQGWDVLRALKADAATSELPVIIVSMIDNRELGLAFGADDYFVKPVDWT
                     RFMRRLREIALRTSAPKRPRLLLIDDDVSVHDILEAELTKQGYQIEKAFNGVEGLEKA
                     ARNPPDVIILDLTMPGMSGFEVAAHLKESEVTARIPILALTAKELTELDREHLRAGFA
                     GVVAKGSSSGKRLIQAIHALDTRPAVSS"
BASE COUNT         1176 a         1776 c         1796 g         1193 t
ORIGIN      
        1 attcttcgac acggtgaaat cggatgctat cgcgatttgg tgccgggttg ctgggttggc
       61 ccgttattcg gctgccggtt ggccggttac ccggcaaacc atgaagagta tgcttgacgc
      121 ctgctccatg gtggcgtgat cgatcaatat gctccggaag gagggtacgt tctgcgaaaa
      181 cttgtcgtgt gcctcctgct tcttgttccc gcggccgcgt ttgccggctt cggtgacgat
      241 ttctatgagg gcctctacgc ccgaggcatg gcccacttcc atgcatccga gtacgaggct
      301 gcgttcaacg accttcatct cgccgcattc ggactcgtcg atcaactgga acgatttgag
      361 accgccgagg cgtatgcggc gatttccgcg aatcggatag ggaacgaagc actcacgcgc
      421 agcgctctgg tcggcatttc gaaggccgac ggcgtccaac cgcactttcg ctcgatcgcg
      481 attcctgaat ccgagagggc agagctcgaa cgggctgccg cgcttctcct gacgaacgag
      541 gagcgggcgg tcttgcgcag cgccggcccg acgcagcgtc ccgccgcctt tcggtaagtt
      601 cgactggcga gcaggtgacg gcaatcgatc tcatcggatc ggcatgctga gatgccgtat
      661 ttggtggaac tcgcggaaag attcctgccg gatgaaaaat ggaggatctt gtgttccctc
      721 cttcaatcat ccaccgatcg atttctcgca acgtcgaagg agagcgaatt ctgcgcaaac
      781 tcgccgtagg tttcctattg ttggcgccct cagtcttgct ggctggcggc gttgacgaat
      841 tctacgaacg gctctacacg cgagggatgg ccgacttccg gacggcggac taccaactgg
      901 cgtacaccga gctgcacaag gcggcattcg gattcgtgga ggagatcgag aaattcgaga
      961 ccgccgaggt ctacgcggcg atcgcggcaa accggcttgg ccactcgggc gaggcgcgcg
     1021 acgcgttact ccgcatcgcc gccgcagaag aggtccagcc acacctgcgg tcgatgacga
     1081 ttccggatga cttgcgcgcc gagctccttc gagcagcagc gactctgctg accaagaagg
     1141 aagcagcagc attcggtatc tccgaagcga tgcaggatgc cgcggcgaaa gaacatccgc
     1201 gagtggaggt accgacgccg agcgtggggc cgaacgttgc ggaaaccgct ccccgaggag
     1261 ccggcgacgc gaacgctcct gatggagccc ccgaagatcc acccgctgct tcatcgtcta
     1321 cacccgccgc cgatccgcct cctgccgcga gtcgcgaaga accagccgcg cagcccggca
     1381 cacggcagtc aatcgctccg atggtgccgc ccacgcagca aacagcgaag aagaccaccg
     1441 atcagcgtct cgacgaggct cagcaggcaa tcgatagcgg cgatcctgac aacgcccgct
     1501 cgatctacga cgcgctcctt caagagccgc agcttccgca tgctccggcc atgcgcctgg
     1561 cggaagggct gtaccgcgta cgcgacttcg ccggagcatc acgcgcgttt catggagttc
     1621 gcgagatcgg caagggtgaa gagcgctatc actactacta cgccgtcgct ctcttcgaaa
     1681 cacgtcacta tcgcgatgcg tgccgcgagc tcgatctcgc cctccccttc atcgaggtaa
     1741 ccgaggatgt cgccggttac cgggagaaga tttacaaggc gacgaagtag ggggggtggg
     1801 gagttggggt attcgcgggg agtcgctgat cgggagtcgc gtgtcggagc agttgatcac
     1861 tcaaacttgg cactttcaaa atggggtcag gtctaaagtt gaaagtgacg aaagggacat
     1921 cgggtccgat ccagtcaacg tattggcact ttcaactttg gacctgaccc tactttgaaa
     1981 gtgcccgtga acggcgaaaa tatggcaggt aaatcaaagc aggacgacga gatgcgagat
     2041 cactacgact tttccggcgg cgtacgtgga aagtacgcgg cacgctatgc cgaagggacg
     2101 aatgttgtcg tgctcgctcc cgacgtggcc gacatgtttc ccgactcaat cgcggtgaac
     2161 gaggcgctcc gtaccttcgt tcgcatgacg accaggacgg tccgggccaa agctgcgccg
     2221 aagaaacgcg ctgggtaatt tggataactg gctcggcact ttcggcactt tcaaaatggg
     2281 gtcaggtctg aagttgaaag tgacgaaatg ggacacgtgt cctgatccag tcagtgtttg
     2341 gcactttcaa cttcagacct gaccccattt gaaagtggat cgatttcggt gggcgtttcc
     2401 actcttgaaa tagcgtcggg accaatttcg gtagtcgttt tgactactca aattaggtcg
     2461 ggactaattt cggtggtcgt tttgaccact gaaattaggt cgagaccaat ttcggtagtc
     2521 gttttgacca ctgaaattag gtcgggtcca atttcggtgg tcgttttgac tactgaaatt
     2581 aggtcgggac caatttcggt ggtcgttttg actactgaaa ttaggccgag actaattccg
     2641 gtggtcgttt tgactactga aattaggtcg ggaccaattt cggtggtcgt tttgactact
     2701 gaaattaggt cggaaccaat ttcggtagtc gttttaactc gtgaaattgg gtcgagacca
     2761 atttcggtag tggtttcgac tcgtgcatct cggtaaaact tgccgaattc atcgagtgat
     2821 ctgcacgatc tgcgtcatct gcggatcaat cagggcgacc tccgcagatg tcacagatgg
     2881 agggtcacag cacaaggatt gacgctctcg gacccccgac ccccgactcc cctcgctcta
     2941 cgaagaaacc gccggcctcg tatcaagcgc gtgaatcgcc tgaatcaggc gcttgcccga
     3001 tgacgaccct ttcgcgacca ctccggcgaa tccggcgcgc aagtgttcgc gatcgagctc
     3061 cgtcagctcc ttcgccgtga gcgcgaggat cggaatgcgc gccgtgacct cgctctcctt
     3121 cagatgcgcg gccacttcga acccgctcat cccgggcatg gtcagatcga gaatgatgac
     3181 gtccggcggg ttcctggcgg ctttctccaa cccttccacg ccgttgaatg ccttctcgat
     3241 ctggtaaccc tgcttcgtca gttccgcctc cagaatgtcg tgaacgctga cgtcgtcgtc
     3301 gatgagcaga aggcgcggcc gcttcggcgc tgatgttcgc aatgcgatct cgcgcagccg
     3361 gcgcatgaag cgcgtccagt cgaccggctt cacgaagtaa tcatccgcac cgaaggccag
     3421 ccccagctca cggttgtcga tcatcgagac gatgatcacc ggcagctcgg aggtcgccgc
     3481 atctgctttg agcgcgcgca aaacatccca tccctgcgcc cccggcagaa cgatgtcgag
     3541 cgtcaccgcc accggtttga tgctgcgcgc gaggcgcatc gcttcgtccc cgttgcgcgc
     3601 ccggatcggc acgtaggcgg ccgactgcag atacgtcgcc agcgtcgagt aggcgacgtc
     3661 ttcgtcttcg actacgagca cgcgctcgcc gggcggaatg accgtgccgt cgacgttgat
     3721 gatcggactg ggaatcgtcg tccctgcgaa gtgaatgggc agcgtgaacg tgaacgtgct
     3781 gcctttgccg atctcgctcg ccacggtcag cgttccaccc tgcaactcga cgaacttccg
     3841 cacgagagag aggccgaggc cggtgccgcc gtactggcgg ttgagggtgc cgtccacctg
     3901 acggaattct tcgaagatca gcgtcaggtt ctccggggcg atgccgatgc cgggatcggt
     3961 gacgtcgatg ctgatcgatt cagcccgctc ctcggtggct tcgacgcgcc gggcgtggat
     4021 ctttacgacc gactcggtcg gcgaaaactt caccgcgttg gagagcaggt tgtaaaggat
     4081 ctgcttgaac ttcgactggt ccgtttcgat cgcggtaacc cccggatcga tctccaccgc
     4141 gaacgagatc gccttgcgcg tcgacatgcc ccgcatgatc tggcagatgc tctcgatggc
     4201 actgcgcacc ggaaacgtct cgggcaacag ctccatcctg cctgcttcca ctttcgagag
     4261 atcgaggatg tcgttgatga tctcgagcag gtgcctgccg gagctgagga tcgagcggat
     4321 gaacgtcaac gacttcgcgt cgatctgcgc ctccaggcgg tcgatgagaa tctccgagaa
     4381 gccgatgatg gcgttcatcg gcgttcgcag ctcgtggctc atgttggcca ggaagcggct
     4441 cttggcctga ttggcctcct cggcctcccg attcttcagt tgcagctcgc gcaggatgcg
     4501 ggctttggaa atggcggaga tggcgtgctc gcgcacgcgc gccagcttgc gcaaatcgct
     4561 gcggctgaaa gcattcgggt cgctgaaatt gtcgaagatg agaaaaccct caatccgccc
     4621 accaagcgag acttccatcg ccagcatcga cttgggaacg cggaaatgct tcgtctgctc
     4681 gctgcccgcg agctcgtgaa aaacatctcc cttgatgaga tacacaccct cttcaagtag
     4741 ctccgcccgt tccgaatagc gccgtgttgc ttcctccgga gtcaggcgaa catcgctgaa
     4801 cgactcctgg tcatagccga gctcggccac gacctccgtc gagccccggt cgtgatcgaa
     4861 ctgtaagaac gctgccgctt ccgtctgagg aaaaagcttc gtgccctgat cgagaagggc
     4921 gcggaacacg ttctcgagca cgagctcgcg gttgatgatc tcgaccatcc ggtcgagcgc
     4981 ttccaactcg tgggttcgcg cggaaacgat gtcttcgagc tcgcggttgc ggcgccgcaa
     5041 acgccggact cgaagccggt tcgcgacgct cgccagcgcc acgatggctg cggcataggc
     5101 gatcatcgcc caccagcgga gccaccacgg cggcatgacc gagaaggtgt aaagctcggg
     5161 aagcgaccat acgccgtcgc tgcttcgcgc catcacggcg aagcgatacg acttcgggaa
     5221 gaggtaggcg gggaggttgg tgtaccgaat ctttgcgtcg gaccgcgcag ccgcccagtc
     5281 gttgtcgtag ccgaagagcc gtgtgcggta gacgacgcgc gactcgtcgc taaaggtcag
     5341 tgcggcgtac gcaatcgaaa cttcgttatt gccccttcgg tcttcgcgaa aggtgatgtc
     5401 gcgaaagcga acgatcggcg gttcggcatt gggctcgcgc agagccggat tgaaaatcga
     5461 gagaccgcgc ggcgtcgcga agtagacccg gccgtcatca ccaacggcaa gcggatcgta
     5521 ggcccacgcc tcgtcatcga tgagaccgtc cgctttcgac acgcgcgtgc gaacgtgaaa
     5581 gtcgctcgta tcgacttcga cgagtccggc gttctgactg acccagaccg acttgccatt
     5641 cggtgacgac atcagtccgg ccaccagact tccacccagc gacgatcccg gaaggctggc
     5701 aacgatgcgc agcggattcg tgtcgaggat ttccaggcca ttcgccgtgc cgctccacaa
     5761 cttgccggcg gcccagagca gcgtgcgaac ggagtcggta cgggcgccgg ttgcatgcga
     5821 ccacgcgggt gcgaagaatc tggacacgtt gcccggcttc aatgccgccg gatcgaacgg
     5881 cactacgctg cgcaggagtc cactgtccgg cgtgccggcc cagacgtaac cggcgccatc
     5941 g
//
LOCUS       DATLVW010000784         6119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_242416, whole genome shotgun sequence.
ACCESSION   DATLVW010000784 DATLVW010000000
VERSION     DATLVW010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242416"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VNN25_22905"
     CDS             complement(<1..242)
                     /locus_tag="VNN25_22905"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HXH94445.1"
                     /translation="MRIKLIYNPMAGRGRASRHHAEAERHLRALGAEVDVHASTSPAD
                     MTQAAADASRGTFDRVVVCGGDGTLHLSVREFDLANG"
     gene            840..2213
                     /locus_tag="VNN25_22910"
     CDS             840..2213
                     /locus_tag="VNN25_22910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXH94446.1"
                     /translation="MKQLRLIVILFLVILACKPAAESQTVDAHQPRQDRTPIPPTVSA
                     SRRTPIVTVAHDVGPAVVNIQTESTIRQRAVDPFFDPFGVFGGQDRTYTSQSLGSGFV
                     WSADGIIVTNNHVVEGASRITVNFSDGTQLPAKLIGVDPDSDVAVLRVDAKNLLTAPI
                     GTSSDLLIGETVIAVGNPFGLSGTVTTGVVSALGRSVPSKEAGRTFTDFIQTDASINP
                     GNSGGPLLNIEGRVIGINTMIFANAQGIGFAIPVDRAKKVIQDIVRFGQVHSAWIGAV
                     TATITPEEARRTGLQTSRGALVARVISGSPAQAAGLRPGDIITAVAGRAIDSREAFST
                     LTATVAAGQPLPLTVLRGGSSQPVSVRPTEAPGDLGLRILWEIAGLRVIDQNHQLVID
                     EVARGSRSENIGLQQGDIIVGANGSEVSSVKALNQVLTQSAERSSVVLQVARGRYVYS
                     LTFPMGT"
     gene            complement(2319..2762)
                     /locus_tag="VNN25_22915"
     CDS             complement(2319..2762)
                     /locus_tag="VNN25_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94447.1"
                     /translation="METRPLPLGIADHRHHYLVSLAAEVHRTYFQKYPPLSVKWGQQI
                     TRKKRRSIRLGSYNHSTSEIRIHPLLDSPEIPAFFIQSVIHHEYLHHVLGASHNRRFH
                     AQERQFRYFRESKEWIRRHLWLLLGTKRNRNLAIPAVVQPQMVLF"
     gene            complement(2803..3138)
                     /locus_tag="VNN25_22920"
     CDS             complement(2803..3138)
                     /locus_tag="VNN25_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94448.1"
                     /translation="MPTTRDDDTERSAAGMDYDFPLRDKASELGNAFAAEGPNALFDA
                     IENMLPESWREHIRTFPIAAVVIGVGAGIFLGMRKGDEIIAAGTAMITAAAMENVTNV
                     MHAATHPGE"
     gene            complement(3147..3431)
                     /locus_tag="VNN25_22925"
     CDS             complement(3147..3431)
                     /locus_tag="VNN25_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HXH94449.1"
                     /translation="MAETTSGAGTGADAGTDSRFGKAKEFVNDKYEAASDAVKNQYNR
                     VREKVEDVDFGAITDQVRSYVRSNPGKALLISVGVGFLVGLLLRRDDHQD"
     gene            complement(3465..3614)
                     /locus_tag="VNN25_22930"
     CDS             complement(3465..3614)
                     /locus_tag="VNN25_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXH94450.1"
                     /translation="MLWTIIAILLILWILGFVSSYTFGGLIHIILVVAIILLVIQLLS
                     GRRRL"
     gene            3677..4567
                     /locus_tag="VNN25_22935"
     CDS             3677..4567
                     /locus_tag="VNN25_22935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH94451.1"
                     /translation="MRIRLEHVTHAYAGSDESAAVQVLGDISLDIASGDFAVLMGASG
                     SGKSTLLNLIGAVDKPTAGRIFLDDSETSVLDDSRLTMLRRRSIGYIFQFFNLIPTLN
                     VFENVAFPLSLANAPRSEVKSRVAEALASVGLGNRATHYPNELSGGEMQRAAIARAVV
                     HNPPLILADEPTGNLDSKNGEMILDLIRSIHKSRRPTIVMATHSERAAAYGDYVIEVS
                     DGRVWGVGSGGWETRESAFGGRESELSSNVSSPTSDSQPSTPSPESEQSQVSSPTPDP
                     RKPTPAAPTPHPPLPTPRKP"
     gene            4564..>6119
                     /locus_tag="VNN25_22940"
     CDS             4564..>6119
                     /locus_tag="VNN25_22940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXH94452.1"
                     /translation="MILRALVLRPILREPLRTTLTILGIAVGVAVVVAIALSNQSALR
                     AFSESVDAVAGRANYQIVSDVAFSEDVLRALQPFWPRGVRFAPVIDAEGIVEPSQQPI
                     RLLGVDLLSDLHFRDYRYASVVTSGKESAATYLSLFGNDSVVVPATFAHDHGLHLGSP
                     LILNIHGVRRTMIVRGLLEARGPATAFNGAIVIADIAVVQAAFGLEGRITRIDLIVPD
                     VSLIPAIERVVPASARLERPSRRNERVEKMLRAFRVNLFALAGVALLVGMFLVYNTVL
                     ISILRRGKDVGVLKTLGTSASQVFAAFLIEGLVFGTVGSVAGIALGRGLAWVILRLIG
                     RTINALYVNSTPEAIELTPGIIAAGIAVGTILSLLSAIQPSIEAAQLRPSLLLSGAVW
                     RRQPRRQDQTQTPAPYLTQVVVAIVCFVIAVLLSRLPAWNGIAVAGYAAVLFVVAGFS
                     ALAALIVSGASRLLRAPYRGAFGIVGELASASIPASLRRTSIASAALSLAIGMMVAVA
                     LMVGSFRETVR"
BASE COUNT         1030 a         1942 c         1906 g         1241 t
ORIGIN      
        1 ccgttggcga ggtcgaattc gcgaacggag aggtgcaggg tgccgtcacc gccacagacg
       61 acgacgcggt cgaatgttcc acgcgatgcg tccgcggcgg cttgcgtcat gtccgccggg
      121 ctcgtgctgg cgtggacatc aacctcagcc ccaagcgccc gcagatgccg ctccgcctcg
      181 gcgtgatgcc gcgacgcgcg gccgcgaccg gccatggggt tgtagatcag cttgatgcgc
      241 aagcggagag tctaaccgcc agcgggccgc tggcgataca gcagccgggc cgatctggag
      301 tctggacctt cgtttcttga gtccggacct tggtttttga gtccggacct tcgttcttga
      361 gtccggacct tcgtttcttg aatccggacc ttcgtttctt gggtccggac cttcgtttct
      421 tgaatccgga ccttggttct cgagtccgga ccttggttct tgggtccgga ccttcgtttc
      481 ttgggtccgg accttggttc ttgagtccgg accttggttt tttgagtccg gacctttgtt
      541 tttgagtccg gaccttggtt tcttgagtcc ggaccttggt ttcttgagtc cggaccttcg
      601 tttcttcagt ccggacgatc gtttcttgag tccggactca agtcttgcga gtccaaacga
      661 cgattttgga atgcatcgag gtaaaatttg ccgaatctgc ggacatctgc gacatctgcg
      721 gataaaaacc gtttccgcag atgtcgcaga taggcggggc cggagcacga gaatgacccc
      781 tgtcgcctga tacgcgcgat tgggaatctc cgacggctcc cgttcgtatg aatcgcagca
      841 tgaagcagct ccgccttatc gtcatcctct ttctcgtcat actcgcctgc aagccggcgg
      901 cggaatcgca gacggtcgat gcgcaccagc cgcggcagga tcggacgccg atccccccca
      961 ccgtaagcgc gtcgcggcgc acgccgatcg tcaccgtcgc gcatgacgtc gggccggcgg
     1021 tcgtcaacat ccagacggag tcgacgatcc ggcagcgcgc cgtcgatccg tttttcgatc
     1081 cgttcggcgt cttcggcggg caggaccgta cctacacgtc gcagtcgctc ggctcgggct
     1141 tcgtctggtc ggcggacgga atcatcgtca cgaacaatca cgtcgtcgag ggcgcgtctc
     1201 gcatcacggt gaactttagc gacggcacgc agctcccggc caaactgatc ggcgtcgatc
     1261 ccgacagcga cgtggcggtg ctccgtgtcg acgcgaagaa cctgctcacc gcgccgatcg
     1321 gaacctcgtc cgacttactc atcggcgaga cggtcatcgc ggtcggcaat ccgttcgggc
     1381 tcagcggcac ggttacgacc ggcgtcgtct ccgcgttggg ccgttccgtt ccgtcaaaag
     1441 aggctggacg caccttcacc gatttcatcc agaccgacgc ctcgatcaat cccggcaact
     1501 ccggcggacc gcttctcaac atcgaagggc gcgtgatcgg catcaacacg atgatcttcg
     1561 ccaacgcgca gggcatcggc ttcgccatcc cggtcgaccg cgcgaagaaa gtgattcagg
     1621 acatcgtccg cttcgggcaa gttcacagcg cctggatcgg cgcggtgacg gcgacgatta
     1681 ctcccgagga ggcgcgtcgc acgggcctcc agacgtcgcg cggcgcgctc gtggcgcggg
     1741 tgatcagcgg atctccggcg caggcagcgg gccttcgccc gggagacatc atcacggcgg
     1801 ttgcgggacg agcgatcgac tcgcgcgaag cattctcaac cctgacggcc acagtagccg
     1861 cgggccagcc gctgccgctc acggtactgc gcggcgggag ctcgcagccc gtctcggttc
     1921 gtccgaccga ggcgccgggc gatctgggac tgcgcatttt gtgggagatc gcagggcttc
     1981 gcgtgatcga ccagaaccac cagctagtga tcgatgaggt agcgcgaggg tcacggagcg
     2041 agaacatcgg gctgcaacag ggagacatca tcgtcggggc aaatggctcc gaggtgtcgt
     2101 cggtcaaagc gctgaatcag gtgctgacgc aatcggcgga acgatcgtcg gtggtgttgc
     2161 aggtggcccg agggcgctat gtctactccc tgacgtttcc gatggggact tgatttgttt
     2221 gatccgcaga tgacgcagat gttcacagat gaaagctatt tgagaagcgt catgcttcgc
     2281 cttttcatct gcgaaccatc tgcgccatct gcggatcact aaaaaagaac catttggggc
     2341 tgaaccaccg ccggtatcgc cagattgcgg tttcgcttgg tacctagcag cagccagagg
     2401 tgtcgacgaa tccactcttt ggactcgcgg aagtagcgga actggcgctc ctgcgcgtga
     2461 aagcggcggt tgtggctcgc gccgagaacg tggtgcaggt actcgtgatg gatcaccgac
     2521 tgaatgaaga acgcggggat ctcgggtgag tcgagcagcg gatggatacg gatctcggag
     2581 gtgctgtggt tgtacgaccc gagccggatg gagcggcgct tcttgcgcgt gatctgctgg
     2641 ccccacttca cgctgagcgg cgggtatttc tgaaagtacg tccgatggac ctcggcggcg
     2701 agcgagacca ggtagtgatg ccggtgatca gcgattccca gcggaagcgg ccgcgtctcc
     2761 attcccactc attgtgcgga gagcacggcc gcttcgcaag agttattcgc cgggatgcgt
     2821 cgccgcgtgc atcacgttcg tgacgttttc catcgccgcg gcggtgatca ttgccgtccc
     2881 ggccgcgatg atctcgtcac cttttcgcat ccccagaaag atgccggcac cgacgccgat
     2941 gacgaccgcc gcgatcggaa acgtgcggat gtgctcgcgc cacgactccg gcagcatgtt
     3001 ctcgatcgca tcgaagagcg cattcggacc ctctgcggca aacgcgttcc ccaattcaga
     3061 agccttatct cgcagcggaa agtcgtagtc catgccggcg gcagagcgtt cggtatcgtc
     3121 gtctcgtgtg gtaggcatca tcactcctag tcctgatggt cgtcgcgccg cagcagcagc
     3181 ccgaccagaa atccaacgcc caccgagatc agcagcgctt tccccgggtt ggaacgaacg
     3241 tagctgcgaa cctgatcggt gatggcgccg aagtctacat cctcgacctt ctcgcggacg
     3301 cggttgtatt gattcttcac cgcatcggaa gctgcttcgt acttgtcgtt gacgaattct
     3361 ttagccttgc cgaatcgtga gtccgttcct gcgtcggcgc cggttcccgc gccgcttgtc
     3421 gtttctgcca tggagtacct ccttgttttg ggctgctcag cctatcagag gcggcgccgc
     3481 cctgacagaa gctgaatgac cagaaggatg atggcgacga cgaggatgat gtgaatcagt
     3541 ccgccgaagg tgtagctgct gacgaagccg aggatccaga ggatgagcag aatggcgatg
     3601 atcgtccaga gcatggtgtg tccccctttc aaaagcgcga tttgcagcga tcgtgccgcg
     3661 cgtacaatcg cccgccatgc gcatccgact cgagcacgta acgcacgcat acgcgggaag
     3721 cgacgagtcg gcggccgtgc aagtcctcgg cgacatctcc ctcgacattg cctcgggcga
     3781 cttcgcagtc ctgatgggcg catcgggcag cggaaagtcg acgctgctca atctcatcgg
     3841 cgcggtagac aagccgaccg caggcagaat ctttctcgat gacagcgaga catccgttct
     3901 cgacgactcg cggctgacga tgctgcgccg gcgatcgatc ggctacatct tccagttctt
     3961 caatctcatt ccgacgctca atgtcttcga gaacgtcgcc ttcccgctgt cgctggccaa
     4021 cgcaccgcgc agcgaggtga agtcgcgcgt agccgaggcg ctggcgagcg taggcctcgg
     4081 caatcgcgcg acgcactacc cgaacgagct ttccggcggc gagatgcagc gcgccgcgat
     4141 cgcccgcgcc gtcgtccaca acccgccgct gattctcgcc gacgaaccaa ccggcaatct
     4201 cgactcgaaa aacggcgaga tgatactcga cctgattcgc agcatccaca agagccgccg
     4261 tccgacgatc gtcatggcca cgcactcgga gcgcgcggcg gcgtatgggg attacgtgat
     4321 tgaggtaagt gacgggaggg tgtggggggt ggggagtggg gggtgggaga cgagggagtc
     4381 ggcgttcggg ggtcgggagt cggagctgtc atcgaacgtc tcttctccca cttctgactc
     4441 tcaaccttcc acgccttcgc ccgagtcgga gcagtcgcag gtttcttctc cgacccccga
     4501 cccccgaaaa ccgactcccg ctgctcccac tccccacccc ccactcccca ccccccgaaa
     4561 accatgattc tccgcgctct cgttctgcgt ccgattctcc gcgagccgct gcggacgacg
     4621 ctcacgattc tcgggatcgc tgttggtgtg gccgtcgtcg tggcgattgc gctttcgaat
     4681 cagagcgcgc ttcgtgcctt cagcgagtcg gtcgatgcgg tcgcgggacg ggccaactat
     4741 cagatcgtgt cggacgtcgc cttcagcgag gacgttctgc gcgcgctgca accgttctgg
     4801 ccgcgcggcg ttcgcttcgc gccggtgatc gatgctgaag gaatcgtcga gccttcgcag
     4861 caaccgatcc gtctcctcgg cgtcgacctc ctctccgacc ttcacttccg cgactaccgc
     4921 tacgccagtg ttgtgacgag cgggaaggag tccgcagcga cctatctcag cctcttcgga
     4981 aacgactcgg tcgtcgttcc cgccaccttc gcgcacgacc acgggctcca tctcggctca
     5041 ccgctgattc tcaatatcca cggcgtgcgc cggacgatga tcgtgcgcgg cttgctcgaa
     5101 gcgcgcggtc cggcgaccgc attcaacggc gccatcgtta tcgccgacat cgccgtcgtg
     5161 caggctgcct tcggcctcga gggacgtatc acgcgcatcg acctgatcgt gccagacgtg
     5221 tcgctgatcc cggcgatcga gcgcgtcgtt ccggccagcg ctcgtctgga gcggccgtcg
     5281 cgccgcaacg agcgcgtcga gaaaatgctg cgggccttcc gcgtcaatct cttcgcgctc
     5341 gccggcgtgg cgctgctggt ggggatgttc cttgtctaca acacggtgct gatctcgatc
     5401 ctgcggcgcg gcaaggatgt cggcgttctg aaaaccctcg gcacctcggc gtcacaggta
     5461 ttcgcagcat tcctgatcga aggtctggtc ttcggaacgg ttggatcggt tgccggtatt
     5521 gcgctcggcc gcggattggc ctgggtgatc ctgcggctga tcggccggac gatcaacgcg
     5581 ttgtatgtga actcaacgcc cgaagcgatc gagctgacgc cgggcatcat cgctgccggc
     5641 atcgccgtgg gaacgatcct ctcgctcctc tcggcgatcc agccatcgat cgaagccgcg
     5701 caactgcgtc ccagtttgtt gctgtccgga gccgtctggc gccggcagcc tcggcgacag
     5761 gatcagacgc aaacgccggc gccgtaccta acgcaagtcg tcgtcgcgat cgtctgcttc
     5821 gtgatcgctg tgctgctctc gcgtctcccg gcgtggaacg gcatcgccgt cgccggttac
     5881 gctgccgtgt tgttcgtcgt ggcgggattc tccgcacttg cagcgctcat cgtctccggt
     5941 gcttctcggc tgctgcgcgc gccgtaccgc ggcgcattcg gcatcgtcgg cgagctggcc
     6001 tccgcgtcga tccccgcctc cctgcgacgc acatcgatcg cgtcggctgc attgtcgctg
     6061 gccatcggca tgatggtcgc ggtcgcgctg atggtgggga gctttcgcga gacggtacg
//
LOCUS       DATLVW010000785         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_281892, whole genome shotgun sequence.
ACCESSION   DATLVW010000785 DATLVW010000000
VERSION     DATLVW010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281892"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VNN25_22945"
     CDS             <1..965
                     /locus_tag="VNN25_22945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH94453.1"
                     /translation="LFLNETKMASLYGPPYVQTIDIPAQEGVGYIRAVAQLKDDPQPP
                     VEDLVMINTPQFMEEVNVHLVELPTTVMRNGRPINDLGENAFKVMDEGKAVKVAKFEH
                     VNNLALSIGLAIDTSASMQPRMSEAQKAASQFFTNVMRKGDRAFLVSFDTQPQLIQRW
                     SPRLADVNAGLGKLRAEESTALYDAVVYSLYNFLGVKGQRALVLVTDGKDTSSKFTFD
                     QALEYARRAAVPIYSIGIGIKASEFDTRYKLSRFSTETGGNTYYIEMASELGHIYEDI
                     QNELRSQYILGFYPPEGVKAGSKWRAVDVKVSDGKAKTIRGYYP"
     gene            962..>2312
                     /locus_tag="VNN25_22950"
     CDS             962..>2312
                     /locus_tag="VNN25_22950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXH94454.1"
                     /translation="MRAAGVALLLVLAGVTAAAQTLPPPASQGEVKLSRRERKDRIAK
                     LPEVYRQFLTDVEPIMQDQERDTFLIMESDAQRDLYIADFWRRHDLTEGTPSGSFQKI
                     YYDRLASAKELFGTLHEDRSRALLVHGEPAERTELDRCHLLQPMELWTYRNVKELGTT
                     THFLFYQPRGGVEYRLWAPYSGMLSPSDTVNDSQDTRYDLISNEVVATTRSRQEAINH
                     VFGIIPYTNTTYLERDCRDAANVFGVITDAQLQRLDSGKLFQPAAVDPEQVKKLLHSV
                     VLPTAGAKKLPAQLTISYPTKQGNRTTAELTILVPRSPLIVKEVGGTKLYSLDVVGEV
                     LKDDKLFENYRYRFDYPADIKTEQLAVVVDRLLQPADYQARIKIADLNGTSEAIVEQR
                     ITVPEVAGSPEKLQKEKESTATLTAIQEKIESHAPTVRILPLPDELLSGTQHIETDIE
                     "
BASE COUNT          497 a          702 c          718 g          395 t
ORIGIN      
        1 acctcttcct gaacgagacg aagatggcct cgctctacgg gccgccgtac gtgcagacga
       61 tcgacattcc ggcgcaggag ggtgtcggct acatcagggc cgtcgcgcaa ttgaaagacg
      121 atccgcagcc tcctgtcgaa gatctcgtga tgatcaacac gccgcagttc atggaggagg
      181 tgaacgtcca cctcgtcgag ctgccgacca ccgtaatgcg caacgggcga ccgatcaacg
      241 acctcggcga gaacgcattc aaggtgatgg atgaagggaa ggcggtgaag gtggcgaagt
      301 tcgagcacgt gaacaatctc gcgctctcga tcggtctggc catcgacacc tcggcctcga
      361 tgcagccgcg gatgtcggag gcgcagaagg cggcttcgca attcttcacg aacgtgatgc
      421 gaaagggcga ccgcgcgttt ctggtgtcat ttgatacgca accgcagctc atccagcgtt
      481 ggtcgccgcg ccttgcggac gtcaacgccg gcctgggaaa actgcgcgcc gaggagtcga
      541 ccgcgctgta cgacgcggtg gtctattcgc tctacaactt cctcggcgtg aagggacagc
      601 gcgcgctggt gctcgtgacc gacggcaagg acacctcttc gaagttcacg ttcgatcagg
      661 cgctcgaata cgcccggcgc gcggcggtgc cgatttactc gatcggtatc ggcatcaagg
      721 cgagcgagtt cgacacgcgc tacaagttga gccgtttctc cacggagact ggcggcaaca
      781 cctactacat cgagatggcc tcggagctcg gccacatcta cgaagacatt cagaacgagc
      841 tgcgctctca atacatcctc ggcttttacc cacccgaggg cgtcaaggca ggatcgaagt
      901 ggcgtgctgt ggatgtcaag gtgagcgacg gaaaggcgaa aacgattcgc ggctattacc
      961 catgagagcc gcgggcgttg cgcttcttct cgtgttggca ggcgtgacgg cggcggcgca
     1021 gacactgccg ccgccggcga gtcagggcga ggtgaagcta tcgcgccgcg agcgcaagga
     1081 tcggatcgcc aaacttcccg aggtctaccg gcagtttctg accgacgtcg agccgatcat
     1141 gcaggatcag gagcgcgaca cgttcctgat tatggagagc gacgcgcaac gtgacctcta
     1201 cattgccgac ttctggcgcc ggcatgattt gaccgaagga acaccgagcg gttcatttca
     1261 aaagatctac tacgaccgcc tggcgtcggc gaaggagttg ttcggcacgc tccatgagga
     1321 tcgctcgcgc gcgctgttgg ttcacggcga gcccgccgag cgtaccgaac tggatcgctg
     1381 ccatcttctc caaccgatgg agctctggac gtaccgcaac gtgaaggagc tcggcaccac
     1441 tacccacttc ctgttctacc agccgcgggg gggcgtcgag taccggttgt gggcgccgta
     1501 cagcggaatg cttagcccct ccgacacggt gaacgactcg caagatacgc gctatgacct
     1561 catttcgaac gaggtggtcg ctacgacgag aagcaggcag gaggcgatca accacgtctt
     1621 cggaatcatc ccctacacga acacgaccta cctcgaacgg gattgccggg atgcggcgaa
     1681 tgtgtttggg gtgatcaccg acgcgcagct ccagaggctc gacagcggaa agcttttcca
     1741 gccggccgcc gtcgatccgg agcaagtcaa gaagctccta cactcagtcg tcctgccgac
     1801 tgcgggcgcg aagaaactcc cggcacaact caccatttcc tatccgacga aacagggcaa
     1861 ccgcacgacg gcggagctga cgattctcgt gccgcggtcg ccgctgatcg tgaaggaagt
     1921 cggaggcacg aagctctaca gcctcgatgt cgtcggcgag gtgctgaagg acgacaagct
     1981 cttcgagaac tatcgttatc gcttcgacta tcccgccgac atcaaaaccg agcaactcgc
     2041 ggtcgtggtc gaccggctcc tccagcccgc cgactaccag gcgcgcatca agatcgcgga
     2101 tctgaacggc acgagcgagg ccatcgtcga gcagcggatc acggtgcccg aggtggcagg
     2161 ctcgccggag aagctgcaga aggagaagga atcgacggcg acgctgacgg cgatccagga
     2221 aaagatcgag tcgcacgcgc cgacggtgcg cattcttccg ctgccggacg aattgctcag
     2281 cggcacgcag cacatcgaaa cggacatcga ag
//
LOCUS       DATLVW010000786         9877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_77073, whole genome shotgun sequence.
ACCESSION   DATLVW010000786 DATLVW010000000
VERSION     DATLVW010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77073"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1516)
                     /locus_tag="VNN25_22955"
     CDS             complement(149..1516)
                     /locus_tag="VNN25_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXH94455.1"
                     /translation="MAYPIMIAGAIGIFLLVRSTGSTLVAPAPAVVAAAGDPAANAEV
                     LLHVLLALVVVIIAARACGALCKRIHQPPVIGEVIAGILLGPSLLGHISPAAFSYLLP
                     ATVAPFLSIIAQIGVILFMFLVGLELDTAQLKHRTHATVAISHASIVAPFVLGAVLAL
                     WLYPKLSTRDVRFTVFALFLGVSLSVTAFPVLARILTDRGIQKTRMGTVSLTCAAVDD
                     VTAWCLLAFLIAVAKANVSAAAVTVALTGLYVAFMMLVARPLIIRAVRAQELRESVSQ
                     SAIAVVVVALLLSTLATEAAGIHAIFGAFLLGAIIPHDSRLARDLTNKIEDLVVVLFL
                     PAFFAFTGMRTQIGLVHGASQWLLCGVIIAAASIGKFGGTFVAARFTGFGSRDAAALG
                     VLMNTRGLMELIVLNIGLDLLILSPTLFAMLVLMAIITTLATTPILQLLTHNATEPDG
                     EPAMV"
     gene            complement(1605..1934)
                     /locus_tag="VNN25_22960"
     CDS             complement(1605..1934)
                     /locus_tag="VNN25_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94456.1"
                     /translation="MPDLTKSQKRAVGELLGDAHEAEIASALVKVEQAIKEWRSGAIL
                     PSEVSERIHEFHKESQQIFKTYNNLDLMFALARAVAFGFVELARVPEDLQPRIKELEA
                     LVKGDDG"
     gene            complement(2118..2522)
                     /locus_tag="VNN25_22965"
     CDS             complement(2118..2522)
                     /locus_tag="VNN25_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94457.1"
                     /translation="MPEPDASKAFAHLHKELVEYQLRLADITSKGATLALLTIGWMLT
                     SESARTFLATSRVGRLSAVAGLTIMSAAYILLAIRMTGVMQSLGRQMQALDYFPFSYY
                     SFRVYTPRVAIATAAIALAPAGVAVALMLFGT"
     gene            complement(2592..2879)
                     /locus_tag="VNN25_22970"
     CDS             complement(2592..2879)
                     /locus_tag="VNN25_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94458.1"
                     /translation="MAVVERLESNVHEEFLSDGGAMVSNLVAFAIVSPKGFDTMLFAH
                     VKGHPYIGDRPILLGDTVTFVLPRNWRNRDMSLDELEGLAFAHSAARHSDR"
     gene            complement(3177..3350)
                     /locus_tag="VNN25_22975"
     CDS             complement(3177..3350)
                     /locus_tag="VNN25_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94459.1"
                     /translation="MTCPAYQHAQLLETQVMSLPVQALRDKSAHTYVIRRDPSPAGTG
                     RIAYAHYTLLGTS"
     gene            complement(3477..3914)
                     /locus_tag="VNN25_22980"
     CDS             complement(3477..3914)
                     /locus_tag="VNN25_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH94460.1"
                     /translation="MFQKERLAARVLIPLSVIAAGSLIFSACNIGCFFLPCDREIDVT
                     ARVTDSSGAPLQGVKVDVQGYKGETDGNGCVKLNGVTTADQIWLKAERPGYAIYDEGK
                     PWAIYEIEVTLERAGSAHHSNAKWHSGSSRLNQTQCVSSGGPD"
     gene            complement(3946..4362)
                     /locus_tag="VNN25_22985"
     CDS             complement(3946..4362)
                     /locus_tag="VNN25_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94461.1"
                     /translation="MNYPRIVLATLGSTVVYFMLGGLLFAATPLTDEFRKYPAVYRTP
                     ESMKGVIPFGIVGMLLSMAALSVLYALVYRGGRGLAEGARFGMLVGAFAVGSFVLHNY
                     VNLNIGLKLTVEQAVAYFIQWTVTGIVIGLIYKAPA"
     gene            complement(4519..4854)
                     /locus_tag="VNN25_22990"
     CDS             complement(4519..4854)
                     /locus_tag="VNN25_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94462.1"
                     /translation="MTRPDPDLVLQRADRKRLKSEYSALFEDLSALLYQVDPARLNFG
                     INPDEYEPEAGTILPRVMELETASEIESVIREELERWFGGGTRIEKATYEDLGAEVLA
                     LLNRYRSSS"
     gene            complement(4990..5508)
                     /locus_tag="VNN25_22995"
     CDS             complement(4990..5508)
                     /locus_tag="VNN25_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94463.1"
                     /translation="MSTVNEVSQSHTDSAQARVDEIRAMRQLIPNFVIPASTSANRSL
                     ITAASLPQQFIELAAMAVKNSTALVRGGGVDPAQIRDLMSFAEAYGPVADELDALAQF
                     IRHSVTAARNTAGSDALTTYALARRLAKRPETAELAPHVNDMRNALGTRFRKAKAHPA
                     PTPAPVPATPLT"
     gene            complement(5531..6244)
                     /locus_tag="VNN25_23000"
     CDS             complement(5531..6244)
                     /locus_tag="VNN25_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94464.1"
                     /translation="MKVTSYRNTARAAADDSWRAGGTSRGLSRKQRQPWRRPLRRTQR
                     AIDSSMRLLDSSRRVIEASETFATKRPVRASREYQRVSSWLSRAAAQLERAVHGLRDT
                     VNDASRAPEGAAEVPGRLIDTTARWIDSAARMAELSDRLDQASSQLLDSARRGAISID
                     RSDECEPTGDSGKAFIAPLLISSRSVQRTLTGRRHRIRFISARRVRFIRSTAAEAARQ
                     IFRGRAPPYVSTVRSNHSS"
     gene            6267..7001
                     /locus_tag="VNN25_23005"
     CDS             6267..7001
                     /locus_tag="VNN25_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94465.1"
                     /translation="MAEKGGHFVKHQLQPRISINMQRGWKDDFSGVDSEEDNVAFYRD
                     GENRDIDSVDRDIKVENLDIAVKNRDIDVENREIAVEELRHRSGKPRHRSGKPSPSQW
                     KTETSQWKTETSQWKTETSQWKTENIEAENRDIAVRNRDIEAENRDIDVENRDIDAEN
                     RGIVVENRDIDVENLDIGVRNLDIDMVDHHIDASNLVVDDADLDVVRSLLLCAFLGGY
                     MHVVTGTLALAASGAAAHDPGYAIEE"
     gene            complement(6957..7448)
                     /locus_tag="VNN25_23010"
     CDS             complement(6957..7448)
                     /locus_tag="VNN25_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94466.1"
                     /translation="MIVIVERRLPTFDAALRVSRSIDVVRKQLESFFAAGRERPKGFV
                     LFDGRFNGEFDGVNSFVVRSYPNAIHSSLRPVIDGYVVANEDGGCDLHIRVTYPRLWW
                     VFPASLLAMMLVGTLFSRGITGDLLFLFPAPAIIAVVTLLPVWLEGSSFLNRVTRIMR
                     GGA"
     gene            complement(7954..8358)
                     /locus_tag="VNN25_23015"
     CDS             complement(7954..8358)
                     /locus_tag="VNN25_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94467.1"
                     /translation="MNEFLRASVSNGNPREQALIRRWWHTEHGGRGTIVWEYCLEGLY
                     ADAIWFPDSGETGKEESGQQTATRFPLKGREIYLCEAKVFLTPELIGQALVYRWYAEK
                     AGATVKGVVIFAESDPQNRRQAAAALGLVVVV"
     gene            complement(8365..8712)
                     /locus_tag="VNN25_23020"
     CDS             complement(8365..8712)
                     /locus_tag="VNN25_23020"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH94468.1"
                     /translation="MNNMRILPVLFLAIMLSSPAVHASHVAGIVKRPVLLQRVEPECS
                     HRCAPVVLEAVIAKDGSVRDAKIVEGTSSECADAAIEALSKWKYEPATLNTQPVDTIW
                     KYRVSRCWITPID"
     gene            complement(8930..9799)
                     /locus_tag="VNN25_23025"
     CDS             complement(8930..9799)
                     /locus_tag="VNN25_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94469.1"
                     /translation="MRKTKQSNAMRPAPIFRRCAFLLLVAFPLIAQDVTLMNRIRPLA
                     GKDLACAAVYSSYKSGRPRLVVAGIDEGNFGDNGELLLIRLPDAASGRGVVLDRMRLE
                     AGAMELSFIPLVDPKDIAVQLHLKHPPSGVTVRVVEGKLEQIADALAMPQNVADLDGD
                     GIPEIITGFVGGAGECGMRGSPHILRWNGNEYADDGKRYAAMIEARVGAPVEELEFDA
                     RSITGDAAAPPRPYVLRLFPQRGVTRVDVTVDDEPVAAGERVLLESDCHTMTAKPSGR
                     HGAAVFAFLEEQR"
BASE COUNT         2121 a         2946 c         3025 g         1785 t
ORIGIN      
        1 cccgcaaagg gacgttcgcc cgccgttccc gctgcgggcg ccaagcgcga agaagtaagg
       61 tcttaagaac gcaccacaga gacactgaga gcacagagtt gcaacctctg tgacctctgt
      121 gtctctgtgg taaaagcctt ttaccagctc agaccatcgc aggctcgccg tccggctcag
      181 tcgcattgtg cgtgagcaac tgaagaatcg gcgtcgtcgc gagggtcgtg atgatcgcca
      241 tcagcaccag catcgcgaag agtgtcggcg agaggatgag cagatcgagg ccgatgttca
      301 ggacgatcag ctccatcagg ccgcgggtgt tcatcaggac gcccagggcg gcggcgtccc
      361 ggctaccgaa tccggtgaac cgcgccgcca cgaacgtgcc gccgaacttg ccgatcgacg
      421 cggccgcgat gatgacgccg caaagcagcc actgcgacgc cccgtgcacg agaccgatct
      481 gcgtgcgcat cccggtgaac gcgaaaaagg ccggcaggaa caggaccacc acgagatcct
      541 cgatcttgtt cgtgagatcg cgcgcgaggc gcgagtcgtg cgggatgatc gcgccgagca
      601 ggaacgcccc gaaaatcgcg tggatccccg cagcctcggt ggccagcgtc gacagcagca
      661 gggcgaccac gacgacggcg atggcgctct gagagaccga ctcgcgcaac tcctgcgcac
      721 gcaccgcgcg gatgatgagc ggccgcgcga cgagcatcat gaaagcgaca tagagaccgg
      781 tgagcgcgac cgtgacggcg gccgcggaga cgttcgcctt cgccacggcg atgaggaacg
      841 ccagcaaaca ccacgcggta acgtcatcga cggcggcgca ggtgagtgac acggtcccca
      901 tccgcgtctt ctggatcccg cggtcggtga ggatccgcgc gagcaccgga aacgccgtca
      961 cggagagaga gacgccgagg aacaaggcga agacggtgaa gcgaacgtcg cgcgtggaga
     1021 gctttggata gagccagagc gccagcacgg cgccgaggac gaagggcgcg acgatgctgg
     1081 catgcgagat ggccaccgtc gcgtgcgtgc ggtgcttgag ttgcgcggtg tcgagctcca
     1141 gcccgaccag gaacatgaag aggatcaccc cgatctgcgc gatgatcgag aggaacggcg
     1201 ccaccgtcgc tggcaggaga taggaaaacg ccgccggcga aatgtgtccg agcagcgagg
     1261 gcccgagcag gatcccggcg atcacctcgc caatcaccgg cggctgatga atgcgtttgc
     1321 aaagcgcacc acaagcgcgc gccgcgatga tcaccacaac aagcgcaagt agcacatgca
     1381 gcagaacctc agcgttagca gcaggatcac cggcggccgc aacaacagca ggagcgggag
     1441 cgacaagcgt agatccagtc gatcgaacca gaaggaagat cccgatcgcc cccgcgatca
     1501 tgatcggata ggccaacgcg cccctccacg cgcgccctcg ggaccggccg actgtcatcc
     1561 gctcaacgat agctcgaccg gtgggggagt ggaaacgaaa gtcgttagcc gtcatcgccc
     1621 ttcacgagtg cctccagctc cttgatacgt ggctgaagat cctccggaac ccttgccagc
     1681 tcgacgaacc cgaatgccac cgcacgtgcc agagcgaaca tcaggtcgag gttgttgtat
     1741 gtcttgaaga tctgttgcga ctccttgtga aactcatgga ttcgctcact gacttcgcta
     1801 ggcagaatcg cgccgcttcg ccattccttg atcgcctgtt cgaccttgac cagtgccgag
     1861 gcgatctctg cctcatgcgc atcaccgagc agttcaccta cggctcgttt ctgggatttc
     1921 gtgaggtcag gcataacggt taatgccggc ttcggccgtg cgaaatagta gcgcaggaat
     1981 gctgaccgtc gggggtgaag acgatctccg agaggccgaa gctgctcacg ttcttgcaag
     2041 ctggacatct agtcaaatgt ccagcttgac ggcgtgtggc gccggccacg cgcatgcgct
     2101 tcgaacgtcg tttgtgctta cgtgccgaat agcatcaaag caaccgcaac accggcagga
     2161 gcgagcgcaa tcgcagccgt ggcgatcgct actcttgggg tatacaccct gaaactgtaa
     2221 taggaaaaag gaaagtaatc gagcgcctgc atctggcgac ccaggctttg catgaccccc
     2281 gtcatccgta tggcgagcag gatgtacgct gcggacatga tcgtgagccc agcaacggcc
     2341 gataatctgc cgacgcggct ggtggcgagg aaggttctgg cggactcgga ggttagcatc
     2401 cagccgatcg tcaacagagc gagagtcgcc ccctttgaag tgatatcggc gagacgcaat
     2461 tggtattcca cgagctcttt gtgaaggtgt gcgaacgcct tgcttgcgtc aggctccggc
     2521 atgatcgctc cagatgtcgc ggttcacgtg gtggcaatac gtatgcgagt atatcaggaa
     2581 cggcgcgctc gttaacgatc ggagtggcga gccgcgctgt gcgcgaatgc aagcccctcg
     2641 agctcatcga gtgacatatc ccggttcctc cagttgcgtg gcagcacgaa ggtcaccgtg
     2701 tctcccagca ggataggacg atcgccaatg taaggatggc ccttgacgtg cgcgaagagc
     2761 atggtatcga agcccttggg gctcacgatg gcgaacgcca ccaggtttga gaccatggcg
     2821 ccaccgtcgg atagaaactc ctcatggaca ttgctctcga gacgctcaac gaccgccacg
     2881 accagttgac cttcataggg tccaggggtc tcagcgccag gcgaggtccc gggagctggt
     2941 gtggaagatt gattcggcgc gtgagcacac ccggagaacg gcattatcag aagacagacc
     3001 gccatcagac acgaccgagc tctgatacga atcgacatgt gcaggcctca acgatgccgg
     3061 ctgccgcctg acggtcagcg cgcaaccgca tcaacagcac gggcttgaag tatagctctc
     3121 gcgacaccga gtcagggcgc ggctactgac tgcgaaggcc ggacttcctt gaagaactag
     3181 ctcgtgccga ggagggtgta atgcgcgtag gcgatgcggc cggtgccagc cggcgacggg
     3241 tcccggcgga tgacataggt gtgagcggac ttgtctcgaa gtgcctgcac gggcaatgac
     3301 atcacttgcg tctcgagtaa ttgcgcgtgc tgatacgccg ggcaggtcac gcaatgcagg
     3361 tagccgaagc ggcttctacg cctgcgccgc tgatattacg gtggccagag ccggcagaat
     3421 catcgctgac attgttctca tgtggaagtc cccatgtggc ggccagacgg tcgaagtcag
     3481 tctggtccgc cgctgctcac gcactgagtc tggttgagcc ggctcgaacc ggaatgccac
     3541 ttggcgttgc tgtggtgagc tgaacctgcg cgctcgagtg tgacttcaat ttcgtagata
     3601 gcccagggct tgccctcatc atagatcgcg tagcccggtc tttccgcttt gagccagatc
     3661 tgatcggcgg tggtcactcc attgagcttg acgcagccgt tgccgtccgt ctctcccttg
     3721 tagccctgga catccacttt caccccctga agaggcgcac cggatgaatc cgtgaccctc
     3781 gctgttacat cgatttcccg gtcacacggt aggaaaaagc aaccaatgtt gcaggccgag
     3841 aagatgagcg agccggcagc aatgacggag agtggaatga ggacgcgtgc ggccaatcgc
     3901 tctttctgga acattgtctc gactccctgc tgatgacgca tcgcgctacg ccggcgcctt
     3961 gtaaatgaga ccgatcacga tgccggtgac ggtccattgg atgaagtaag ccactgcctg
     4021 ctccaccgtc agtttcagcc cgatgttcag attgacgtaa ttgtgcaata cgaacgagcc
     4081 gaccgcgaac gccccgacga gcatgccaaa gcgggcgcct tcggccaggc cgcggccccc
     4141 gcgataaacc aatgcgtaga gaaccgaaag cgccgccatc gacaagagca tcccgacgat
     4201 tccgaaaggg atgaccccct tcatcgactc gggtgtgcga tagacggccg gatactttct
     4261 gaattcgtct gtcagaggag tcgctgcgaa cagcagacct ccgagcatga aatagacgac
     4321 tgtggatccg agggtagcca ggacgatcct tgggtaattc attgcatgcc tcccgaggag
     4381 cgtgacttgc caggcgttcg tgcggtctga ccgatccaag ctcacccggc ttggagtggc
     4441 acgggaaata gtagcgcact cggacgcagc cggccgtggg cacgctggcg ggaagcggct
     4501 gtcagcggcc aggcagcact acgagcttga ccggtagcgg ttgagaagcg cgagaacctc
     4561 agccccgaga tcctcatacg tggctttctc gatccgagtg ccgcctccga accaccgctc
     4621 gagctcttcg cgaatgacgg actcgatctc gctcgcagtt tcgagctcca tcactctcgg
     4681 aaggattgtg ccggcctccg gctcgtattc gtcgggattg attccgaagt tcagtcgcgc
     4741 ggggtcaacc tgatagagaa gcgccgaaag atcctcgaag agagcactgt attcactctt
     4801 cagccgcttg cggtcagccc gctggaggac gaggtctgga tcgggacgtg tcattcaggc
     4861 cgcataacgg atacaactca caggtttgga gccacgcgag atggtaccgt caaccaaagc
     4921 tccacgatac aaaaaatgcg cgggcgcgcc ttcggacgcg cccgcgcggg gcttactgca
     4981 cgaactgcat tacgtcagcg gcgtcgctgg caccggcgcc ggagtgggcg ccggatgagc
     5041 tttggccttg cggaaccgag tgccgagggc gttgcgcatg tcattgacgt gcggcgccag
     5101 ctcggcggtc tcgggacgct tggccagacg gcgcgccagg gcataggtgg tgagcgcatc
     5161 gctccccgcc gtgttcctgg ccgccgtcac gctgtgacgg atgaactgcg ccagcgcatc
     5221 caactcgtcc gccaccggcc cataagcctc ggcgaaactc atcagatcgc ggatctgcgc
     5281 cggatcgacg ccgcctccgc ggacgagcgc tgtgctgttc ttcactgcca tcgccgccag
     5341 ctcgatgaat tgctggggca gcgatgcggc agtgatgagg ctgcggttgg cgctcgttga
     5401 cgctggaatt acaaaattgg gtatcagttg ccgcatggcg cgaatctcat cgacgcgcgc
     5461 ctgcgcgcta tccgtatgcg actgcgaaac ttcattcact gtggacacgt ggtcctcctt
     5521 ttctttcttg tcaactgctg tggttagagc gcacagtgga gacataaggc ggagcacggc
     5581 ctctgaagat ttgccttgct gcctcggctg cggtgctgcg aatgaaccgc acgcgccgag
     5641 cggaaatgaa ccgaatacgg tgacgcctgc cagtcaaggt tctttggacg gagcgcgacg
     5701 aaatcagcaa cggagcgatg aacgccttcc cggagtctcc ggtgggctcg cactcgtctg
     5761 accgatcgat ggaaatcgcg ccgcgtctgg cggaatcgag caactggctg gatgcctggt
     5821 ccagacggtc ggagagctcc gccatccgcg ccgcactgtc gatccagcgt gccgtggtat
     5881 cgatcagacg tcccggaacc tcggcggccc cttccggcgc ccgcgacgca tcattgaccg
     5941 tgtcgcgcaa accgtgaacg gcacgctcca actgtgccgc cgcccgagag agccagctcg
     6001 aaacgcgctg atactcacgc gatgccctga caggacgctt cgtagcgaag gtctccgagg
     6061 cctcgatgac acggcgtgag gagtcgagca gccgcatcga cgaatcgatg gcccgctgcg
     6121 tcctccgaag cggccggcgc cacggctgcc gctgtttccg gctcaatccg cgcgaggtcc
     6181 caccggcgcg ccacgaatca tccgcggccg ctctcgctgt gtttctgtag cttgtgactt
     6241 tcatactctt acttagacgc agccaggtgg ccgaaaaagg gggtcatttt gtaaaacatc
     6301 agctacagcc acgaatatcc atcaatatgc agagaggttg gaaagacgac ttttcaggcg
     6361 tcgactcaga ggaagacaat gtcgcgttct atcgcgatgg ggagaatcga gacatcgatt
     6421 cggtcgatcg agacatcaag gtcgagaatc tcgacatcgc agtgaaaaat cgagacatcg
     6481 atgtggaaaa tcgagagatc gcagtggaag aacttcgaca tcgcagtgga aaaccaagac
     6541 atcgcagtgg aaaaccgagc ccctcgcagt ggaaaaccga gacatcgcag tggaagaccg
     6601 agacatcgca gtggaaaact gagacatcgc agtggaagac cgagaacatc gaagcggaaa
     6661 accgagacat cgcggtgagg aaccgagaca tcgaagcaga aaaccgagac atcgatgtgg
     6721 aaaaccgaga catcgatgcg gaaaatcgag gcatcgttgt ggaaaaccga gacatcgatg
     6781 tggaaaactt ggacatcggt gttcgaaatc tggacatcga tatggtcgat catcacatcg
     6841 atgcctcaaa tctcgtcgtc gatgacgcgg accttgatgt cgtgcggtcg ctgctcctgt
     6901 gtgccttcct aggaggctac atgcacgtgg tgaccgggac gctcgcactc gcggcgtcag
     6961 gcgccgccgc gcatgatcct ggttacgcga ttgaggaatg acgaaccctc gagccaaacc
     7021 gggagaaggg tcacgacggc gatgatcgca ggcgctggaa acagaaacag aaggtcgcca
     7081 gtgattcctc gggagaagag ggttccgacc agcatcatgg ccagaagcga cgcgggaaag
     7141 acccaccaga gtcgaggata cgtcaccctg atatgcaagt cgcaaccgcc atcttcattg
     7201 gccaccacgt atccgtcaat gaccggacgc agagagctat gaatggcgtt cggatacgag
     7261 cgcacgacga aggagttgac tccatcgaac tcgccgttga agcgcccgtc gaataggacg
     7321 aacccctttg gtcgctcacg gcccgcggcg aagaacgact ccagttgctt cctgacgacg
     7381 tcgatcgacc ggctcacacg aagagccgcg tcgaacgtcg gcagccgtcg ttccacgatt
     7441 acgatcactg gactttgcat tgttcgaggc ggctaatccg catttctcgc accattcagc
     7501 aacgcgcttc taccaactcc ctacaagtgt gagaaatgca ggttagggtt gagtgacatc
     7561 gggatgatcg tccaaaccaa gattggtgtc atcctgagac cgccggagcc gcccgctcga
     7621 atcgcgatgc ttacgcagtg cgtgctcggc ggcgcaggac gtcgaaggat ctcaaacttc
     7681 gcagatcggg ttcgtccagc gcgagcgatc gtgattcaaa gaccgcttga acgagatctt
     7741 tgcattttga gatccttcgc cgtcctgcgg cgccgaggat ggtctgcgcg agagcaacgt
     7801 gctgcgcggg cgcctccggc aggctcagga tgacacgtca cgttgtgagc gcaccgccaa
     7861 cgcctcctgg agttcaggat ggcaacaatc acgtcacgca atgcccattg ctcgcacctt
     7921 gcagcaatgc gcggccgcgc ggggagcagc gactcaaacg acgacaacga ggccgagcgc
     7981 ggcggcggct tgcctgcggt tttgcggatc gctctctgcg aagatgacaa cacccttgac
     8041 ggtcgctccc gccttctctg cgtaccaccg gtagaccaat gcttgaccga tcagctcggg
     8101 tgtcaagaaa acttttgcct cgcaaaggta gatttcacga cccttcaacg gaaagcgggt
     8161 ggccgtttgc tgccccgact cctcttttcc ggtctcaccg ctgtcaggaa accagatggc
     8221 gtcagcgtaa aggccttcaa ggcagtactc ccagacaatc gtgcctctcc cgccgtgctc
     8281 ggtgtgccac cagcgtcgaa tcagagcttg ctcgcgcgga ttcccgttcg acacggacgc
     8341 acggaggaac tcgttcatgc ggttttagtc aatcggcgtg atccagcagc ggctaacgcg
     8401 atacttccaa atggtgtcga caggctgcgt attcagcgtt gctggctcgt acttccattt
     8461 cgaaagagcc tcgatggcgg cgtcagcgca ctcggaagac gtgccttcca cgatcttggc
     8521 gtcgcgaaca gaaccgtcct tggcgatgac ggcctcgaga acgactggcg cgcagcgatg
     8581 gctgcactct ggctcaacgc gttgcagcag aaccggccgc ttgacgattc cagcgacgtg
     8641 gctggcatga acagccggcg aagagagcat gattgcgagg aagaggactg gaaggattcg
     8701 catattgttc atcggctaat ggttccgctt cggtggtcgt tgttcaatac ggacagctta
     8761 tcgcgctcga cgacgcaaaa ggcgaccaca gagacacaga ggacacagag gacacagagg
     8821 tttctctgtg ctctctgtgc ctctgtggtg aaccggtttt ccaaagaaat tcgcgcctga
     8881 aacggatcga tccaagctca cctgcctgga agtgcggcgc tggtgacctt cagcgttgtt
     8941 cttccaggaa agcaaatacg gcagcgccat gccggcccga gggcttcgct gtcatcgtgt
     9001 ggcagtcgct ctccaggagc acgcgctctc cggcggcgac cggctcgtcg tcaacggtta
     9061 cgtcgacacg ggtcacaccc cgctgcggga agagacgcag cacataaggc cggggaggag
     9121 ccgcggcatc gccggtgatc gatctggcgt cgaactcgag ctcttcgaca ggcgcaccga
     9181 cccgcgcctc gatcatcgct gcgtagcgtt tgccatcatc cgcgtactcg tttccgttcc
     9241 agcgaagaat gtgaggactt cccctcatcc cgcactctcc ggcaccgccc acgaagccgg
     9301 tgatgatctc tggaatgccg tcgccatcga ggtccgcgac gttttggggc atggcgagag
     9361 cgtcggcaat ttgctccagc tttccctcga cgacgcgcac agtgacgccc gacggcggat
     9421 gcttgaggtg gagctgcacg gcaatgtctt tcggatcgac aagagggatg aacgacagct
     9481 ccatcgcccc ggcctcgaga cgcattcgat cgaggaccac gccgcggccg cttgcggcat
     9541 cgggaaggcg gatgagcaac agctcaccgt tgtcgccgaa gtttccctcg tcgatgccgg
     9601 cgacgacgag acgcggacgc ccactcttgt agctgctgta aaccgcggca caggcaaggt
     9661 ctttaccggc gagcggccgg atgcgattca tcagtgttac gtcctgcgca atcagaggaa
     9721 acgccaccag aaggagaaag gcgcatcggc ggaagatcgg ggcgggtctc atggcgttgc
     9781 tctgttttgt ctttctcatc gctctggctc tgtcgcatgc cgtccgttta tcgtggtcgc
     9841 cgaagcaaaa agcaaccaca gagacacaga gaacaca
//
LOCUS       DATLVW010000787        16242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_443745, whole genome shotgun sequence.
ACCESSION   DATLVW010000787 DATLVW010000000
VERSION     DATLVW010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16242
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443745"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1173..1913
                     /locus_tag="VNN25_23030"
     CDS             1173..1913
                     /locus_tag="VNN25_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94470.1"
                     /translation="MDDDNRSQSSSQPQPVNPSTPQPVQAAGDPAPQEPLTPEELAPI
                     LLAKIDEIEAMIPNFQHHDKNDIRRVAAMARFARELMVPTIATVTSFGPAAERNMFDV
                     AAGQLSLRRRDAFAPVIQRLAALTGGVQFTVDSALAESVAQAFTVFGWGKSYAKRPDA
                     APLHPYMAEMTHVVKKVLNRRKPSPPAHPGPAPAPTTGSSELPLGARGFLASNMVRSK
                     PVDEPEPVGEDEYSDEFNEALDRAVKDE"
     gene            2378..3394
                     /locus_tag="VNN25_23035"
     CDS             2378..3394
                     /locus_tag="VNN25_23035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH94471.1"
                     /translation="MLIAEDDENFGMWLSAMARRLGLDVATAVDGAQAFDWLRGGDFD
                     MLISDFEMPKKDGLELIAAVRAEPSTSGLYAVMLTAHDRPAVKINALTLGYDDFLAKG
                     CTEVEVAAKVAAARRMLARQRAHDADAREWRDIANQDELTRVATRRFFFEQAERQMAG
                     LNSIGIVLFDVDEFKAINDTYGHLTGDRVLKDIGALFLRRTRTDDVIARYGGDEFVLL
                     VLNLGVDETRCVAERLVSELSELQWTVGEVTLRINVTMGIGCSALIEEPTVERLLDVA
                     DHELYARKWLKKNPTVTAPEVYQYPPSDRSLVTEIRTGPAATLSPLADRKRTRRNPEP
                     PSAE"
     gene            complement(4035..6659)
                     /locus_tag="VNN25_23040"
     CDS             complement(4035..6659)
                     /locus_tag="VNN25_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94472.1"
                     /translation="MKKMRRNKRLSKRDRGSALLVSLMVIVGLSLLGLGFVAISETES
                     AIARNQQNALQTQAVAEAGAKLVVEWFQDPVWGQNIGGMPLNTSANANLGATKVTRVS
                     GDYSGVYKPSSTTLLFDKPYRPDPSNRFFADENTGDIVINRTTDLTTITRVNSVLFGS
                     STEDTRSGEITEIRIYAPPSIGGVLKQNGSALNPDGTPQMFWTGPVQRFGLATIKVTA
                     MQFRDPSGDKSAASNIISRHSIRLVVGELPLPIPAGPIQGNANVSFGGNFQVHWGLET
                     STGDLQTSVARVALPWANAFERPHFEHGYEPGTGVDSVSVMSGGSGYTTPPTITIAPP
                     PAGGTQATATATLTNGILTSVTMTPVTGFGSGYDMQNPPAITVTGGGGTGASLQANVG
                     SEAWPQTAGQFDNADFFHELLGKSFEDPWYGARSIGDNSTDGTNAPGDAPMCNNYNIQ
                     ADDQSTSTPYWAFQWQSVNNYPWQKRIIFPVIKYDYWKRIAKQGRGYKGIYYFTYDTT
                     SSSTAPTFKRFGSGLSKPMSYWANSLATTGSGLGAGLYFFDSIDGSNPQSLTGAAKTA
                     KLTPAESWNSQDFNKGFLMEGFVYMNTVSFGTTGQGKAPAYDANFPGEPFRDIGAPQW
                     DVASNSWKQCPPNSGIPCRVGAGDGLFTCPDLNNNGKCDIVVMQAPTWASHDPNTISH
                     VSPFTMPNGNVAFVEKTWKSPAVATAEYGAPCTLPNKTTYDGTNPAATDCSEPHEPYL
                     NLIYPDQAMDSNNKPAIVTVGWEDPKKQTYRAKLAGVTCSASSSVNDCTSNGYDLDGA
                     RVDLNVILYGVLYNEGQYKAEGDAAYYGSVLIQDNIVSGSGTADVWFDEKLIKGSWAP
                     PNMPRVIVFNEQTDEEQQ"
     gene            complement(6671..9826)
                     /locus_tag="VNN25_23045"
     CDS             complement(6671..9826)
                     /locus_tag="VNN25_23045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH94473.1"
                     /translation="MKHRRSTPRTQRGFTLVEILVTTAIFAIIMIAALSVYDQSNKVF
                     KTSTEQADLQQSTRIGFDKLVSDLRMAGFDYSRGGIPNGAGTFAQPDEQIEYAGPTAI
                     VFRANFNYTSASAQGNGLEPAYTPYDPATKAAIFPYVTTSNTEIVIYALKSADTTKNT
                     STIKFYADTDIPRHAYPGSPTNGVEQTVTVSGIDTTNANPPYTLYRMTVADVLNGSAG
                     TPVAENIRALHFFYYTDPKGTTLLKDPANPTADITTGHDAGGNTFPTTYTVTLPDGTS
                     TTYNTGAIGGDGQYDANNSNTVNVGDRNMRALIQSIKVDLTGINANPDAKYTNPTETI
                     GAIKSYRQYQLSALIVPRNLGLTGFPEPSYTPPGPPTITGVCVSACAVPVICWSPPTT
                     GGPVLQYRIAWDTSTGGSFSSGVNITDMTINSYVIGDDGVMDPSLTWYYRMTAQNDNG
                     SSVPSDLVAISPTNRTRPLPPSGLAATKATTQAAITPGNVDYAIGLSWFAPSQNDPAK
                     SGSNCTGGGCATDGTTIPPQEVIRYKVARGLTSTFDPTKGEGVIILDISSKSQPAVVA
                     PGSSVPWRDTAAGLSSLFPPGTCVPYYYRVLAQDRCAANPKYNASGQTSDSVSDWSPA
                     VGSLTQVSGQAYDAGPTVKASAPATVNIGAASLCPAIGTCTIYLNWSQVGTDNAGNAI
                     GVDKYRIQRFKKIVTDPDYTLDTSFGTGGYLDQSGKSQINTGILSYTDSPPGADSNGQ
                     PLYYKYIVTANDCRLGIDSAPAYYPPLCAPQINPDIQQNGAKSATSGDSPSQAWVFNS
                     GDTITVKPASGGVAITKVVFSLYTYPGLVLVDTKTPAASPNFVYTYGDQSDNQTYYMK
                     MDITSSTPCTETHVKYIQDQPAASCSFQNQAAPVPGESAGQGSALIATSTMAITNGGA
                     DTMTLNSQSYQLTFIDPDGNHADIKLLSVTFSTVVSGTTNVWTDTLTTPPGPSTITHF
                     FPTASPVLTVAASGTLTVTVKWTYSKQDNGSPPKNRPPIAKSPMTKFCIGYTIPSEAG
                     VVKKCNAVGQAASSLNPTSCD"
     gene            complement(9829..10425)
                     /locus_tag="VNN25_23050"
     CDS             complement(9829..10425)
                     /locus_tag="VNN25_23050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXH94474.1"
                     /translation="MKARQQGYSLIEVLVAIAITSVVLLTVLTLFYMGRRNVYSGKQT
                     TYANSIGTRILEDLSAMSADDLLTNFVIDDNTPLTTVTLSPVPGAAGGKIDFTNSILR
                     NTDTISSTTEAWTSTAGPADGYLTNWKKYLDPSKLTGATVGIIITPRAPTNLVNGQPA
                     VAPITTAQFTKVRVYIAWDEAANRRRYAFFDTTKVRSY"
     gene            complement(10439..10978)
                     /locus_tag="VNN25_23055"
     CDS             complement(10439..10978)
                     /locus_tag="VNN25_23055"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXH94475.1"
                     /translation="MNPQKSRQSGFSLVELLTVVAVIGILALVTVPNFISFYQMNKVK
                     AAMRNFTSDLRTARQMAITSGMQAKITFTPDTSATAASRAYTFWTGNSAFSATPTWTQ
                     KGRTKYLEDVVYFPKTGQTFNSTGGVYSIVFFPDGSVGMPTGTPAPTTASVILKTDQK
                     VSKPLYTIDISPSGRVYAH"
     gene            complement(11114..12796)
                     /locus_tag="VNN25_23060"
     CDS             complement(11114..12796)
                     /locus_tag="VNN25_23060"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94476.1"
                     /translation="MVRFVRAPLTRVGFRRDVRLFLTGLVGFLVALIFILLLLLRTNL
                     AHTEEAIDRSHQMIADVSAEAVNRSAPAGLDTELLVLRGRFNIAGIGVTARNGRTFES
                     GLREGDFDVVTRLAGPGTLTLRFDASARRAAHRDFLLTAAICILAASLGIALLLFYLP
                     RVTGPIEQMLDDARELGGPGDQEETAYLIDTFRQSIATLKSQEEELKRLHDREKTRAD
                     ELERVTGALTRSLTSGLIVLDATGAVADINAAGREILGIDAAASVAGGTLPDAIPLRV
                     FAETLRDAFEQRSAMNRIEINDRQAGTGEPVTIGLTTVPLRNEGDTLLGFLALFTDLT
                     PIRSLEARVQEMTTLAQLGEISAGIAHEFRSSLSTILGYMKLARKGGVDDVADSRLRN
                     AEKEAALLLKAIERLLAFAGPVQLNAESIDLRALLGEQIAHITDIDPSIAVTLEGPAV
                     VIQGDRILLSRALENLLNNAADAVRQNGGGSISVTLRDKPPSMTIADDGVGFDPADGP
                     RFFLPFQSNKPSGFGMGLPLAKKIVLLHGGTLRLNGEPGKGAVATIEFGTAV"
     gene            complement(12769..13569)
                     /locus_tag="VNN25_23065"
     CDS             complement(12769..13569)
                     /locus_tag="VNN25_23065"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HXH94477.1"
                     /translation="MTFLLGFYAFATGAIVGSFLNVVIHRYPREESIVFPGSHCPHCD
                     AAIRWFDNIPIISFAVLLGRCRACGAGIAWRYPLVELANALFYLAVFLRTGPTVAFVV
                     VATIISMTIALIYIDADIQLLPDIIDVPGIAIGLGVGALGLGVLHPDLILADSLLDSL
                     FGALLGAAIPLVIIGTYWLVRHAEGMGMGDVKMLAMIGAVTGWRAVPGVLLGASVTGA
                     LIVLPAALRSRDGMRLAIPFGVFLGIAFLGVLFFGPTLSGWYVSFVLR"
     gene            13623..13937
                     /locus_tag="VNN25_23070"
     CDS             13623..13937
                     /locus_tag="VNN25_23070"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HXH94478.1"
                     /translation="MKYVYIALCVAGVAAPYYFFVPFVLEHGLNMSLFMRQLIATPVS
                     AFFGADVVVSSIALWAFIAYERRKRPVRLWWLCIIANLAVGVSLALPLFLLLRHSSEP
                     AS"
     gene            14094..15950
                     /locus_tag="VNN25_23075"
     CDS             14094..15950
                     /locus_tag="VNN25_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXH94479.1"
                     /translation="MVSLSLLALGIVYGDIGTSPLYALRECFYGSHPVALTPANVLGI
                     LSLIFWSLVLVVMLKYHVYVLRFDNRGEGGILALMGLVGMDKSRSAAVRRTLIVLGVF
                     GAALLYGDGMITPAISVLSAVEGLEIATPFFKPYVIPLTIVVLIVLFFFQRRGTAGVG
                     AVFGPIMLVWFSTLALMGIRGILWQPSVLFAINPMHAIRFFMANGPHGFLVLGAVFLV
                     TTGGEALYADLGHFGEKPIQIDWFNIVGPSLVLHYFGQGALLIRQPSAAHNPFYLLAP
                     KWALYPLVGLATVATVIASQAIISGVFSLTRQAIQLGYLPRMEIIHTSRAEIGQVYMP
                     NVNWTLMLATITLVIGFRSSTNLAGAYGVAVSTTMIITTILAYVVARDKLHWSVALAG
                     AITAGFLFGDLAFLGANLFKIADGGWFPLLIATVVFIIMTTWRKGRQILNARLREGSL
                     SPEMFVTSLRAHSPVRVPGTAVFMHRTREVIPSALLHSLKHYKALHKQVILLTVLTEE
                     VSHLREDERLQFEELGEGIFRITCRYGYMEEADIPALLERVAKERHMAIPPMDTTYFL
                     GRETLIVTARPSGMAMWREKLFASMMRNAESAARFFRLPPNRVVELGAQIEF"
     gene            complement(15997..>16242)
                     /locus_tag="VNN25_23080"
     CDS             complement(15997..>16242)
                     /locus_tag="VNN25_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94480.1"
                     /translation="RNLPLVTWSDPDDRICPRLKSDQFTGVRGVWFLHVDAYGSFDSY
                     RSEFWDLNTAERVLIEIERSEPLSSDLQALKDAIEFE"
BASE COUNT         2978 a         4661 c         5320 g         3283 t
ORIGIN      
        1 ctattcgacg gatcacatca cgaaaggttc ctgacatgta ctgactcttc gacggatcac
       61 gtcacgaaag gtttctgacg tgtcctgact cttcgacgga tcacgtcacg aaaggttcct
      121 gatgtgtcct gactcttcga cggatcacgt cgcgaaaggt tcccgacgtg cctgactctt
      181 cgacgcagca ggtcgcgaaa agttcccgat gcggcggagt gtgcttacaa cgggggaggg
      241 aagggtccga ttccgggagt gccggaggcg gcggatgttg cgggtttgaa agcctttatc
      301 tgcgtacatc tgcgccatct gcggatcggt cctaccccga ttgatccgca gatgacgcag
      361 atgtagcaga tgggtgcatt cggcaaattt taccgtgttc ggactcaaga gatttgagtc
      421 gggactcaca aactttgagt cgggacacaa aagatttgtc tcgggacaca aaagatttgt
      481 gtcgggactc aaagatttgc ctcgggattc aaaggatttg agtcgggact caaagatttg
      541 tctcgggact caagagattt gtgtcgggac tcaaagattt gcctcgagac tcaaaggatt
      601 tgagtcggga ctcaaagttt tgcctcggga ctcaaaggat ttgagtcggg actcaaagat
      661 ttgtctcagg acacaaaaga tttgtctcgg gacacaaaga tttgtgtcgg gacacaaagg
      721 atttgcctcg ggactcaaag gatttgagtc ggggctcaaa gttttgcctc gggactcaaa
      781 agatttgagt cgggactcaa agatttgcct cgggactcaa gagatttgag tcgggactca
      841 aagacttgcc tcgggactca aaagactcga gtcaggactc aaggatttga ctcgggactc
      901 gaggaacttg agtcgggact caaaagtttg agtccggatt cggaaaccat gagtcctgag
      961 caagattctc cggagtcggc gtcaacaagg aatccatcgg cttcacgttg tcgggtttct
     1021 tctcggccgc gtaacctctg gcgggctcat acgtcagtgc tatcgagtac tcagagatcc
     1081 cggaccggcg gcgcgtcgcc gacggccacg gaaagttgtt aaccaaccca cgtggagtgg
     1141 tccaggccgc gtggttttat gaggattcga tgatggacga cgacaatcgc agtcaaagca
     1201 gtagtcaacc gcaacccgtc aacccgtcaa ccccacaacc cgtccaggct gccggcgatc
     1261 cggcaccgca ggaacccctc accccggagg agcttgcgcc aatactcctc gccaagatcg
     1321 acgagatcga ggcaatgatc cccaatttcc agcatcacga taagaacgac atccggcgcg
     1381 tggccgcaat ggcccgcttc gctcgggaac tgatggtgcc caccatcgcc acggtcacgt
     1441 catttggacc ggcagcggaa cggaacatgt tcgacgtcgc ggcaggccaa ctgtccctgc
     1501 gccggcgcga tgcctttgcc ccggtgatcc agcgcctcgc cgcgctgacc ggcggcgtgc
     1561 agttcaccgt cgactcggct ttggccgagt cggtggcgca ggctttcacc gtctttggat
     1621 gggggaagtc ttacgccaag cgtcccgatg ctgcgccact gcatccgtac atggcggaga
     1681 tgacgcacgt ggtgaagaag gttctcaacc gtcgtaagcc atcgccaccg gctcaccccg
     1741 ggccggcgcc ggcgccgacc accgggtcgt cagaactacc actcggagcc aggggcttcc
     1801 tcgcttcgaa catggttcgt tccaagccgg tagatgagcc ggagccggtg ggtgaggatg
     1861 agtattccga tgaattcaac gaggcgttgg atcgcgcggt gaaggacgag tagttcgctc
     1921 gttttgcgtt catgccgccc ctcgctagtg cagaggaggg gcggcggggt tggttggggt
     1981 ggggagtcgt ttgtcggggg tcgggggtcg gagcactcga aatgatcaac tggctccgac
     2041 acccgactcc cgatcatcga ctcacggttc aaggggagta gggagtgggg ggtgggagag
     2101 cgagggagtc gcttgtcggg ggtcgcgggt cggagcgttg gattgatcaa ttgctccgac
     2161 acccgacacc cgactcccgg agaactccga cccgcgactc ccgatcagcg accctcggat
     2221 ctcccactcc ccactcccca ctccccaccc cccttgtgac ccccatcacg cacggcccca
     2281 gctctggcgg cgatattgaa tgtttggtta ccagttgagc gccgcccttc gccgccgtag
     2341 tgaccttcct ccggagtcgt catcggaacg tattcgtgtg ctcattgccg aagatgacga
     2401 aaacttcggg atgtggcttt cggcgatggc ccggcgcctt gggctcgacg ttgcgaccgc
     2461 ggtggacggc gcccaggcat tcgactggct gcggggcggc gacttcgaca tgctcatctc
     2521 cgacttcgag atgccgaaga aggacgggct ggaactgatc gcggcggttc gcgcggagcc
     2581 ctcgaccagt ggcttgtatg ccgtgatgct gacggcgcac gaccggccgg ccgtgaagat
     2641 caacgcgctg accctcggct acgacgactt cctggccaag ggatgcaccg aggtcgaagt
     2701 ggccgccaag gtagccgcgg cgcgacggat gctggcgcgg cagcgcgctc atgatgccga
     2761 tgcccgcgag tggcgcgaca tcgccaacca ggacgagctg acaagagttg ccacgcgccg
     2821 gttctttttc gaacaggccg agcgtcagat ggccggcttg aacagtatcg gcatcgtcct
     2881 ttttgacgtg gatgaattca aagcaatcaa cgacacctac ggtcatctca ccggcgaccg
     2941 ggtgctgaaa gatatcggtg cgttgtttct ccgccggacg cgaaccgacg acgtcatcgc
     3001 ccgttacggc ggcgatgagt tcgtgctgct cgtgctgaac ctcggcgtgg acgaaacccg
     3061 ttgcgtcgcc gaacggctgg tctcagagct gtcggagctg cagtggacgg tcggcgaggt
     3121 gacgctgcgc atcaacgtga cgatgggaat cggctgctcc gcgctcatcg aagagccgac
     3181 agtggaacgc ctgctcgatg tcgccgatca cgagttgtac gcaaggaagt ggctgaaaaa
     3241 gaatcccacg gtcacggcgc ccgaggtgta ccagtatccg ccctcggatc gatccctggt
     3301 gacggagatc aggaccggcc ccgcggccac attgagcccg ttagcggacc gaaaacgcac
     3361 ccgccgcaac cccgagcccc cctccgccga gtaaaggagt gcaggcgtct cgcctaggcg
     3421 tctcgcctgc ggccggcggg cgtctcgccc ggcggtgtgg agcgtctttt tcagaggctc
     3481 cttatcgaga aggtagcgaa gccggaaggg gatcgaacgc agagcctcca accacgcctc
     3541 gtccgactga atacggcatg cgcggatgtc gtactcaatg gccccctcat ccggccttcg
     3601 gccaccttct cccccacaag aagcgtgggg gagaaggcgc tcgattggaa ggttgcgata
     3661 agagggtgga gaaaagcctg gttacccagg ctaacgctcg cgaacccctt cctgacccca
     3721 cgccaaaccc aacgccaaac ccaacgccaa acccacggct aacgctgccg aacccccgca
     3781 tttctcccac gtctttttca gcgacacctt ttgtatcgag cgccttctcc ccccgcgctt
     3841 tttgcggggg gagaaggtgg cgaagccgga tgagggggga tcagataccc agagcgacca
     3901 cccacgcctc gtccgactga agacggcatg cgcggatgtc gtactcgttg gaacaccttt
     3961 ggcgctcgat ggaaaagtgg gaagaaccag ccaatgggag ggcggagaaa acctctccgc
     4021 cctcccaccc tactttattg ctgttcctcg tcggtctgtt cgttgaagac aatgacgcgc
     4081 ggcatgtttg gcggcgccca cgagcccttg atcagctttt cgtcgaacca gacgtcggcc
     4141 gtgccgctgc cactgacaat attgtcctga atcaggaccg agccgtagta ggcggcatcg
     4201 ccttcagcct tgtactgccc ttcattgtag agaacaccgt agaggatgac gttcagatcg
     4261 acgcgcgcgc catcgaggtc atagccattc gacgtgcagt cgttcacgct cgagctggcc
     4321 gaacaggtga cgcctgcaag cttcgcccgg tacgtctgct tcttcgggtc ctcccatccc
     4381 acagtcacga tggccggctt gttgttgctg tccatagcct gatcggggta aatcaaattc
     4441 aggtagggtt catgcggctc ggagcaatcc gtggcggccg ggttcgtacc atcgtacgtc
     4501 gtcttgttcg gaagggtaca tggcgctccg tactcggcgg tggccacagc gggggacttc
     4561 catgtcttct cgacaaaggc gacgttgccg ttcggcatcg tgaacggcga gacgtgggat
     4621 atcgtattgg ggtcatgcga ggcccaggtg ggcgcctgca tcaccacgat gtcgcactta
     4681 ccgttgttat tgagatcggg gcaggtgaat aggccgtcgc cggcgccgac acggcaagga
     4741 atgcccgaat tgggggggca ttgcttccac gaattgctgg cgacgtccca ttggggagcg
     4801 ccgatgtcgc ggaacggttc gccggggaag ttcgcatcgt acgccggcgc cttaccttgc
     4861 cctgtcgtgc cgaacgaaac ggtgttcatg taaacaaagc cttccatgag gaaacctttg
     4921 ttgaagtcct gtgagttcca ggactctgcc ggcgtcagct tggcggtctt ggccgcaccg
     4981 gtcaacgact gcgggttcga gccatcgatc gagtcgaaga agtagaggcc agcgccgagg
     5041 ccggagccgg tggtggccaa tgagttcgcc cagtacgaca tcggctttga cagaccggaa
     5101 ccgaagcgct tgaacgtggg ggcggtgctg ctggatgtcg tgtcatacgt gaagtaatag
     5161 atgcccttgt aaccgcgtcc ctgtttggcg attctcttcc agtagtcgta cttgatcacc
     5221 gggaagatga ttcgtttctg ccacgggtag ttgttcaccg actgccattg gaacgcccag
     5281 taaggggtgc tcgtggactg gtcgtcggcc tggatgttgt agttgttgca catcggggca
     5341 tccccgggcg cgttcgtacc atcggtgctg ttgtcaccga tcgagcgcgc cccgtaccag
     5401 ggatcttcga agctcttacc gagcaattcg tggaaaaagt cagcgttgtc gaactgcccg
     5461 gctgtctgcg gccacgcttc ggagccaacg ttcgcttgca gggaggcgcc ggttccgcca
     5521 ccgccggtga cggtaatcgc gggggggttc tgcatgtcgt aacccgaacc gaagcccgtc
     5581 accggcgtca tcgtgaccga ggtgaggatg ccgttcgtca gggttgctgt agcggttgcc
     5641 tgggtgccgc ccgccggagg aggagcgatc gtgatcgtgg gtggggtggt gtagccgctg
     5701 ccaccgctca tgacggagac ggaatcgacg ccggtaccag gttcatagcc gtgctcgaaa
     5761 tgaggacgtt cgaaagcatt cgcccacggg agggcgactc tggcaacgga cgtctgcagg
     5821 tctccggtgg aggtctccag gccccagtgg acctggaagt tgccgccgaa agagacgttg
     5881 gcgttgccct gaatcggacc agccggaatg ggcaggggaa gctccccgac gacgaggcgg
     5941 atggaatgac gggaaatgat gttgctggcg gcgctcttgt ctccgctggg atcgcggaac
     6001 tgcattgcag tcaccttgat cgtggccagc ccgaagcgtt gtaccggtcc ggtccagaac
     6061 atctgcggcg ttccgtccgg attgagggcg ctgccattct gcttcaggac gccgccgatg
     6121 ctgggcggag cgtagatccg gatttcggtg atctcgccgc tgcgggtgtc ttccgtggag
     6181 gatccgaaca ggacactatt gactctggtg atggtggtca ggtctgtcgt acggttgatg
     6241 acgatatcgc ctgtattctc gtcggcgaaa aaacggttcg agggatcggg gcggtacggc
     6301 ttgtcgaaga gcaacgtcgt cgacgacggc ttgtagacgc cgctgtagtc gccggagacg
     6361 cgcgtcactt tcgtagcacc gaggttggcg tttgcagatg tgttcagcgg cattccgccg
     6421 atgttttgtc cccataccgg gtcctggaac cattcgacga cgagcttcgc ccccgcctcg
     6481 gctaccgcct gggtctgaag agcgttctgc tgattgcgcg cgatcgcgct ctcggtctcc
     6541 gagatggcaa cgaagccgag gccgagcagc gagaggccga caatgaccat cagactgacg
     6601 agcagcgcag agccgcggtc gcgtttcgaa aggcgcttgt tacgtcgcat cttcttcatg
     6661 gccatcatcc tcaatcgcag gaagtcgggt tcaacgacga tgccgcctgc ccgaccgcat
     6721 tgcatttctt caccacgccg gcctcgctcg gaatcgtgta gccgatgcag aacttcgtca
     6781 tcggcgactt cgcgatgggt ggtcggttct taggcggact gccgttgtcc tgcttcgagt
     6841 acgtccactt gaccgtcacg gtcagcgtgc cggacgctgc tacggtgagt accggcgagg
     6901 cggtcgggaa gaagtgggtg atcgtgctcg gtccaggcgg tgtggtcagc gtatccgtcc
     6961 acacgttggt cgtgcccgag acgaccgtcg agaacgtgac cgacagcaac ttgatgtcag
     7021 cgtgattccc gtcaggatcg atgaacgtga gctggtagct ttgactattg agcgtcatcg
     7081 tgtctgcgcc accgttcgtg atcgccatgg tggacgtggc aatgagtgcg ctgccttgtc
     7141 cggcgctttc ccccggcacc ggcgcggcct ggttctggaa cgagcaggaa gctgccggct
     7201 gatcctggat gtatttaacg tgtgtctccg tgcagggtgt gctgctcgtg atgtccattt
     7261 tcatgtaata agtctgattg tcggattggt ctccgtatgt gtagacgaag ttcggcgacg
     7321 cggcaggtgt cttcgtgtcg acgaggacca gacccgggta ggtgtagagg ctgaacacca
     7381 ccttagtgat tgccacgccg ccgctggctg gcttgacggt gatggtgtcg ccggaattga
     7441 agacccatgc ctgtgaaggc gagtcgccgg aggttgcgct ctttgcgcca ttctgttgaa
     7501 tgtccggatt gatctgcggg gcgcagaggg gaggatagta cgcgggcgcc gagtcgatgc
     7561 cgagccggca gtcgttggcg gtgacgatgt acttgtaata gagcggctgg ccattggagt
     7621 cggcgcccgg aggggagtcg gtataggaga ggatgccggt attaatttgt gatttaccgc
     7681 tctgatcgag gtagccacca gtgccgaagg aggtgtccag agtgtagtcg gggtctgtga
     7741 cgatcttctt gaagcgttga atgcggtact tgtcgactcc aatggcattt ccggcgttgt
     7801 cggtaccgac ctgggaccag ttcagataaa tcgtgcaggt cccgatggca gggcaaagcg
     7861 aggccgcgcc gatgttgacg gtcgcaggcg cggaagcctt tacggtcggt ccggcgtcgt
     7921 aggcctggcc cgagacctgg gtgaggcttc caacggccgg cgaccagtcg ctgaccgagt
     7981 cactggtctg tccactggcg ttgtattttg gattggctgc gcagcggtcc tgcgccagga
     8041 cgcggtagta gtacggaacg caagtgccgg gagggaaaag cgagctcaag ccagcggctg
     8101 tgtcgcgcca cggaacggat gagccgggtg cgacgaccgc gggctgcgac ttcgaggaga
     8161 tgtcgaggat gatcacgcct tcgcctttgg tggggtcgaa ggttgaggtc aatcctcggg
     8221 cgaccttgta acggatgact tcttgcggcg ggatcgttgt tccgtcggtc gcgcagccac
     8281 cgcccgtgca gttactcccc gacttagccg ggtcattctg cgacggcgca aaccacgaca
     8341 gaccgatcgc gtaatcgacg ttgccaggcg taatggcagc ttgcgttgtg gccttggtcg
     8401 ccgcgaggcc ggatggcggc agcggccgcg tcctgttcgt gggcgatatc gcgacaagat
     8461 cgctgggaac gctcgatccg ttgtcgttct gcgccgtcat cctgtaatac caggtcaggg
     8521 acggatccat gactccgtca tccccgatga cataggagtt gatggtcatg tccgtgatgt
     8581 tgacgccgct ggagaacgag ccgcctgtgg aggtatccca cgcgatgcgg tattgcagca
     8641 ccgggccgcc cgtggttggg ggtgaccagc agatcacagg aacggcgcag gcgctgacgc
     8701 atacgccggt gattgtgggc ggccctggcg gtgtatagct cggctcgggg aaaccggtca
     8761 ggccgaggtt tctcgggacg atgagtgcgg aaagctggta ttgacgatag gacttgatgg
     8821 cgccgatcgt ctcggttggg ttcgtgtact ttgcatcggg gttggcgttg attccggtca
     8881 ggtcgacttt gatcgactgg atgagggcgc gcatgttgcg gtcgcccacg ttcaccgtgt
     8941 tgctgttgtt ggcgtcgtac tggccgtcac cgccgatcgc accggtgttg taggttgtcg
     9001 atgttccatc cggcaacgtg accgtgtacg tcgtggggaa ggtgtttccg cctgcatcgt
     9061 ggccggtggt gatgtcggct gtcgggttgg cgggatcttt gaggagcgtt gttcctttcg
     9121 ggtcggtgta gtagaagaaa tggagcgcgc ggatgttttc tgccaccgga gttccagcgc
     9181 ttccattgag aacatcggcc acggtcatcc ggtaaagcgt gtacggcggg ttggcgtttg
     9241 tcgtgtcgat gccgctgacg gtgaccgtct gttccacgcc attggtgggg gagccgggat
     9301 aggcatggcg ggggatgtcg gtgtctgcgt aaaacttgat cgtgctcgtg ttcttggtcg
     9361 tatcggccga tttgagcgcg tagatgacga tctcggtgtt gctggtcgtg acgtagggga
     9421 agatggccgc cttggtcgcg gggtcgtagg gcgtgtaggc gggctcaagg ccgttcccct
     9481 gcgcggaagc ggatgtgtag ttgaagttgg cgcggaagac gatcgcggtc ggaccggcgt
     9541 attcgatctg ttcgtcgggt tgggcgaatg ttccggcacc gttcggaata ccgccgcggc
     9601 tgtagtcgaa gccggccatg cgaaggtcgc tgaccagttt gtcgaagccg atgcgcgtgc
     9661 tttgctgcag gtcggcctgc tcggtcgagg tcttgaagac cttgttgctc tggtcataga
     9721 ccgaaagcgc agcgatcatg atgatggcga agatcgccgt cgtcacgagg atctcgacga
     9781 gcgtaaagcc gcgctgtgta cgcggagtgc tccggcggtg tttcatggtc aatagctcct
     9841 gactttggtg gtgtcgaaga aggcgtagcg gcggcggttg gccgcctcat cccacgcgat
     9901 ataaacgcgc accttggtga actgcgctgt ggtgatgggt gcaacggcgg gttgtccgtt
     9961 cacaaggttg gtcggggcac ggggcgtgat gatgatgccg actgtggctc cggtgagctt
    10021 ggatggatcg aggtacttct tccagttcgt gagatagcca tccgccgggc cggctgtcga
    10081 cgtccacgcc tcggtggtgc tgctgatggt gtccgtgttg cggaggatgc tgttggtgaa
    10141 gtcaatcttg ccgccggccg cgcctggtac gggactgagc gtcactgtgg tgagcggcgt
    10201 gttgtcgtcg atcacgaagt tggtgagcag atcgtcggcc gacatcgccg agagatcttc
    10261 gagaatgcgc gtgccgatgg agttggcgta ggtggtctgt ttgccgctgt aaacgttccg
    10321 ccggcccata tagaagagcg tgaggaccgt gagcagaacg acactcgtga tggcaatggc
    10381 cacgagtact tcgatcaggc tgtaaccttg ctggcgtgct ttcatcgcaa ttcgttcctc
    10441 aatgcgcata gacgcgccca ctcggggaga tgtcgatggt gtagagcggc ttggacacct
    10501 tttgatcggt cttcagaatg accgaggctg tcgttggggc aggagtgcca gtgggcatac
    10561 ccacacttcc gtcggggaag aagacgatgc tgtagacgcc gcctgtggag ttgaaggtct
    10621 gaccggtctt gggaaaatag acgacgtctt cgagatattt ggtcctgccc ttctgcgtcc
    10681 aggtgggagt ggcactgaag gcgctgttcc ccgtccagaa ggtgtaggcg cgcgaggcag
    10741 ccgtggcgga tgtgtcgggc gtgaacgtga tcttcgcctg catgccgctg gtgatggcca
    10801 tttgcctcgc cgtgcgcagg tcgctggtga agttgcgcat cgccgccttg actttgttca
    10861 tctggtagaa gctgatgaag ttcggcaccg ttacgagcgc gaggatgccg atgacagcga
    10921 cgaccgtgag caactcgacg agcgagaagc ctgactgacg ggatttctgc ggattcattc
    10981 gggccgcctt tctgcgagct gggtgccacg ggccgaccca gcatctaagc tcatgaaaag
    11041 aagaaggttt gatctagtgg tggcaacgcc gatcgttggc gtcagcacga tgattggtac
    11101 ataaagtaac agactatacc gccgtcccga actcgatcgt ggccacggct cccttgcccg
    11161 gctcgccgtt cagccgcagg gttcccccat gcagcagaac gatcttcttc gccagcggaa
    11221 gccccattcc gaagccgctc ggcttgttcg actggaaggg aaggaagaac cggggcccgt
    11281 ccgcgggatc gaagccaacg ccgtcgtcag cgatcgtcat cgacggcggc ttgtcgcgca
    11341 gggtcacgga gatcgacccg ccgccattct ggcgaacggc atcggcggcg ttgttcagaa
    11401 ggttctccag cgcgcggctg agaaggatgc ggtcgccctg aatgacgacc gccggccctt
    11461 cgagcgtgac ggcgatcgat gggtcgatgt cggtgatatg cgcgatctgt tccccgagga
    11521 gcgcgcgaag atcgatcgat tcggcgttga gctgcacggg ccctgcgaac gcgaggaggc
    11581 gttcgatggc cttgagcagc agcgcggcct ccttctcggc gttccggagc cgcgagtcgg
    11641 cgacgtcatc gacgcccccc ttgcgcgcca gtttcatgta gccgaggatc gtggagagcg
    11701 agctgcggaa ctcgtgggcg atcccggcgg agatctcgcc gagctgcgcc agcgtcgtca
    11761 tctcctgcac gcgcgcctcg aggctgcgga tcggcgtgag gtcggtgaag agcgccagaa
    11821 atccgagaag agtgtctccc tcgttccgaa gcgggaccgt ggtgagtccg atcgtgacgg
    11881 gctcacctgt gcccgcctga cggtcgttga tctcgatgcg gttcatcgcg gagcgctgct
    11941 cgaacgcatc gcgcagcgtt tccgcgaaca cgcgcagagg aatcgcgtct ggcagggtgc
    12001 ctccggccac ggacgccgcg gcgtcgattc cgaggatctc gcgccctgcg gcgttgatgt
    12061 cggcgacagc gccggttgcg tcgagcacga tcagtccgga ggtgaggctg cgggtgagcg
    12121 cgccggtgac gcgctccagc tcatccgcgc gcgtcttctc gcggtcgtga agacgcttga
    12181 gctcttcctc ctggctcttc agcgtggcga tcgactgacg gaaggtgtcg atgaggtagg
    12241 cggtctcttc ctggtcgccc ggtccgccca gttcccgggc atcatcgagc atttgctcga
    12301 tcggtccggt tacccgagga aggtaaaaga ggagcaacgc gataccgaga ctcgccgcaa
    12361 ggatgcagat cgcggccgtg aggaggaagt cgcgatgcgc ggcacggcgt gccgaggcat
    12421 cgaaccgcag agtgagcgtg ccgggaccgg cgagcctggt gaccacatcg aagtcgccct
    12481 cacgcaaacc tgattcgaat gttcgtccat tgcgggcggt gacgccgatg ccggcaatgt
    12541 tgaaacgccc ccgcagcacg agcagctcgg tgtccaaccc ggccggtgca ctccggttca
    12601 ccgcctcggc tgacacatcc gcaatcatct gatgcgacct gtcgatggcc tcctcggtat
    12661 gcgccagatt ggtccgcagc aagagaagaa ggatgaagat cagcgccacc aggaagccga
    12721 ccagcccggt gaggaaaaga cgaacgtccc ggcgaaaccc gacgcgggtc agcggagcac
    12781 gaacgaaacg taccatcccg agagtgtagg gccgaagaag agaacgccca agaaggcgat
    12841 gccgaggaag acgccgaatg ggatggcgag ccgcatgccg tcgcggcttc gcagcgctgc
    12901 cggcagcacg atcagcgcgc cggtcacaga cgcgccgagc agaactcccg gcacggcgcg
    12961 ccatccggtg acggcgccga tcatggccag catcttcacg tcgcccatcc ccatcccctc
    13021 ggcatggcga acgagccaat aggtgccgat gatgaccagc ggaatggcgg cgccgaggag
    13081 cgcaccgaag agcgagtcga gaagcgagtc ggccaggatc aggtcagggt gaagcacgcc
    13141 cagtccgaga gcgcccacgc cgaggccgat ggcgatgccg ggaacgtcga tgatgtcggg
    13201 aagcaactgg atgtcggcgt cgatgtagat cagcgcgatc gtcatcgaga tgatcgttgc
    13261 cacgacgacg aaggcgacgg tcggcccggt gcgaagaaag accgcgagat agaaaagggc
    13321 gttggcgagc tccacgagcg gatagcgcca tgcgatgccg gcgccgcacg cgcggcagcg
    13381 tccgagaagg acggcgaaac tgatgatggg gatgttgtcg aaccagcgaa tggcagcatc
    13441 gcagtgcgga cagtggctcc ccggaaagac gatcgactct tcgcgcggat agcgatggat
    13501 gacgacgttg agaaagctgc cgacgatcgc accggtggcg aatgcgtaaa agccgaggag
    13561 gaacgtcatc cggtgaggat tgtacctgcc gcttcgaacg ggctcggcgc taccatgagg
    13621 tcatgaagta cgtctacatc gctctctgcg tcgccggagt tgccgctccg tactatttct
    13681 ttgtgccgtt cgtcctcgag cacgggttga acatgtcgct gttcatgcgc cagctcatag
    13741 ccacgccggt gtcggccttt ttcggcgcgg acgtggtcgt gtcatcgatc gcgctctggg
    13801 cattcatcgc ctacgagcgc cgcaaacggc cggtgcggct ctggtggttg tgcatcatcg
    13861 ccaatctggc ggttggcgtc tcactggcct tgccgctgtt tctcctgctt cgccacagct
    13921 cggaaccggc cagctgatga tcggtgacat gctttaggta ttcttaatga gacggcatca
    13981 tccacgcacc gtacactccg ccgttcgcgc cgaaaggcgc acaggggaga tcacggttga
    14041 ctgaggttcc ggcgcccgca caccacgaac gcgaagagcc gtcagggcgc cgtctggttt
    14101 cgctgtctct gctggcactc ggcatcgtct acggcgacat cggcacgagt cccctttacg
    14161 cgctgcggga gtgcttctac ggatcgcatc ctgtggccct cacacccgcg aacgtcctcg
    14221 gcatcctctc cctcatcttc tggtcgctgg tcctcgtggt gatgctcaag taccacgtct
    14281 atgtgctgcg gttcgacaac cgaggagagg gtggaatcct cgcgctgatg gggctcgtgg
    14341 ggatggacaa gagccggagc gccgcggtgc gcagaacgtt gatcgtcctc ggcgtctttg
    14401 gcgcggcgct gctctacggc gatggcatga tcacgcccgc gatctcggtg ctcagcgcgg
    14461 tggaagggct ggagatcgcc acaccatttt tcaagccgta cgtgatcccg ctgacgatcg
    14521 tggtgctgat cgtcctgttc ttctttcagc gccgtgggac ggccggcgtc ggcgcggtct
    14581 tcgggccgat catgctggtg tggttctcga cgctggcgct gatgggcatt cgcggcattc
    14641 tctggcagcc gtcggtgctc ttcgcgatca atccgatgca tgcgattcgt ttcttcatgg
    14701 cgaacggacc ccacgggttt ctcgtgctcg gcgcggtctt tctcgtgacc accggcggcg
    14761 aagcgctcta cgccgacctc gggcacttcg gcgagaagcc gatccagatc gactggttca
    14821 acatcgtcgg accgtcactg gtcctccact acttcggcca gggagcgctg ctgatccgac
    14881 agccgtccgc ggcgcacaat ccgttctatc tgcttgcgcc gaagtgggca ctctatccac
    14941 tggtcggtct ggcgacggtg gccacagtca tcgcgtcgca ggccatcatc tccggcgttt
    15001 tctcgctgac acggcaggcc attcagctcg gctatctgcc gcgcatggag atcatccaca
    15061 cgtcgcgcgc cgagatcggc caggtctaca tgccgaacgt gaactggacg ctgatgctgg
    15121 cgacgatcac gctggtgatc ggctttcgca gctccacgaa tctggcgggc gcgtacggcg
    15181 tcgcagtcag cacgacgatg atcatcacga cgatcctggc ctatgtcgtg gcgcgcgaca
    15241 agctgcactg gagcgtcgct ctggcgggcg cgatcacggc gggattcctg tttggcgatc
    15301 tggccttcct cggcgcgaac ctcttcaaga tcgccgacgg cggctggttc ccgcttctca
    15361 tcgctacggt cgtcttcatc atcatgacga cgtggcggaa ggggaggcag attctcaacg
    15421 cacgccttcg cgagggctcg ctctcgcccg agatgttcgt gacgtcgctg cgcgcgcact
    15481 cgcccgtgcg ggtgcccggc acggcggtct tcatgcaccg cacgcgcgaa gtgattccct
    15541 cggcattgct gcacagtctc aagcactaca aagcgctgca caaacaggtc atcctcctca
    15601 cggtactcac ggaggaagtc tcacaccttc gcgaggacga acgcctgcaa ttcgaagagc
    15661 tcggcgaagg cattttccgt atcacctgcc gctacggata catggaagag gcggacatcc
    15721 cggcgttgct cgagcgggtc gccaaggagc gtcacatggc catcccgccg atggacacca
    15781 cctacttcct cggccgcgaa acgctgatcg tgacggcccg cccgagcggc atggccatgt
    15841 ggcgcgagaa gctcttcgcc tcgatgatgc gcaacgccga aagcgcagcc cgctttttcc
    15901 gcctcccgcc aaaccgggtg gtggagcttg gcgcgcagat cgagttttga agtcgcgaag
    15961 gggagtcgga tcgcgctgcg ctaggcggcg agcacgctac tcaaactcga tggcatcctt
    16021 gagagcctgc aaatcggaag atagcggctc cgaccgttcg atctctatga gaacgcgttc
    16081 cgcagtattc agatcccaga actcggagcg atagctgtcg aaagatccgt aagcatcaac
    16141 gtgaaggaac caaacgccgc gcacgcccgt gaactggtcg gacttcaagc gcgggcatat
    16201 gcgatcgtct ggatccgacc atgtgaccaa gggcagattg cg
//
LOCUS       DATLVW010000788        15516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_486177, whole genome shotgun sequence.
ACCESSION   DATLVW010000788 DATLVW010000000
VERSION     DATLVW010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486177"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VNN25_23085"
     CDS             complement(<1..1052)
                     /locus_tag="VNN25_23085"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94481.1"
                     /translation="MTIYSVVEGSSFARVLIMRASIGVICLLLVVAPAFAADRPVGVV
                     TTVAGVPGLAGFTDGPVAVATFNRPTWVDVAPVSGSPARPGDIFVVDRGNQSIRRISA
                     TGVSTYQVAYLDRPSYVAFDFGGPLGGGIVMEPPHSGCGSGQYDSGMFVSSSGSEQLV
                     LVSMAGSVAARDDVSPLIGLANTPGSVDGTAGYAYSKANEAQFRTPTGLARSWVYADP
                     FPFATRRLYIADTGNHTIRQIRFTGSFEGCPQTRVVETIAGAAGQAGSLDGAGASARF
                     NSPRGLAAGPDGSVFVADTGNHTIRRIAPDRTVTTIAGEPGVAGNDDGIARSAHLNSP
                     SGIDVNAKGEIFIADTG"
     gene            complement(1085..1735)
                     /locus_tag="VNN25_23090"
     CDS             complement(1085..1735)
                     /locus_tag="VNN25_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94482.1"
                     /translation="MRAIVLSLLATAAAFRLAAQAGPSISPKVITMHAGENAGITGSQ
                     HPGGLTCGFPYRYDFISDNPAVAIVHGFASGSSACHPDQVPDNGVVSVTAVAPGVAHV
                     RASGCCGELSTITVLPPIHPVAIQAQATRVLLGQPVIVTALLPDNNQAPLFAWYRGRI
                     GDFSSPLGLSSDPRLTFIGNDLGVSYVWVQAFAGSATSSAEIGIEFFIQPRRRAAH"
     gene            2139..3602
                     /locus_tag="VNN25_23095"
     CDS             2139..3602
                     /locus_tag="VNN25_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94483.1"
                     /translation="MTISGVAQTDPASRDPFETEHSFSKSVIHDLDARSFKDLDARAA
                     DIRKRRAMFDSGAWKLWWFVDAFNIREPALHGVEPQSVETLIHEWIATTGSVTSRVAL
                     AQFHLGFADSLPRNAWQDHEKHVAAATQILDSLQEEGRCDIDCLYLQICAHNLGGWSD
                     PLRKVLKTEPGYWHAFSLATYFLAPRWGGSTREIEKFAAEAADATRTTMGESVYMKAT
                     AMALSTCACEPTYDWKRLRKGFEEFQSRFPSSRVNFYSFARLAFEAHDREMFRRLIAN
                     PFIESWQFQEERKWAMEPDPPVSSRSTNLARVALTELTTSGHTEHPILFYYESGGKTF
                     GASVAPSTIDQWTWNRRQVTSSVRVPLEQSATPSIVFDAEGETALSPFHIRSVEPIIL
                     ELVTAITCRVFENACVPVRFQARIVGQNNENLTLRVLEPPGFDQAIGAPIIDDRESLL
                     GIVHSVTRNEDGSLELSAEPIHFVLGVNRTQTPIGAK"
     gene            complement(3700..4251)
                     /locus_tag="VNN25_23100"
     CDS             complement(3700..4251)
                     /locus_tag="VNN25_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94484.1"
                     /translation="MSTPKEVSNTDPPVSHTAAAQALVLEVRAMRERIPYFVFPESKR
                     ARRQIASVASLPAPFIELSAVAVANSNSLVRGGAADPEKVRDMMSFAEAYLPFADELD
                     AMSSFVRHTVASAKHEAGREALTTYALAQRLAKQPETADLAPHVADMKNALGKRSRKA
                     KAPKAAPPKATATVEPPPELERK"
     gene            complement(4289..4915)
                     /locus_tag="VNN25_23105"
     CDS             complement(4289..4915)
                     /locus_tag="VNN25_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94485.1"
                     /translation="MSSYGTERKRRRFWGRPFRRTLRAIGRSLRLIDASCRAVDAAEG
                     FAAERPLRAARNFELASDWLREITTHLSYAARGLSVMAGRIASTPSLPSSAPEELAAA
                     TSSWIEAVRKMESLSIRCDDSYIALLEAMAGGALLLDLDELLGDERVPAPRRIAMHRP
                     PERRLPRNSSCIQFLHKRRQRSARSTAAEGARRICRGRAPPFLATRTL"
     gene            5264..5512
                     /locus_tag="VNN25_23110"
     CDS             5264..5512
                     /locus_tag="VNN25_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94486.1"
                     /translation="MPSMARVVAFDGMERCHRWYCSLPSMASGDAIDGMDDAFDGMGR
                     CLRWFWTMPSMVLGVAIDAMGDAIDGIEHGHQSRAAEA"
     gene            complement(5684..5839)
                     /locus_tag="VNN25_23115"
     CDS             complement(5684..5839)
                     /locus_tag="VNN25_23115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94487.1"
                     /translation="MADEKRVTETETKTKNDMFGDPKETKTTTKETKTDSSGNEQETK
                     TEYKEKR"
     gene            complement(6029..6463)
                     /locus_tag="VNN25_23120"
     CDS             complement(6029..6463)
                     /locus_tag="VNN25_23120"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH94488.1"
                     /translation="MQKSDCAQIVRLDTPKDPVAALRADIAGGALPDFALLIPDLTHD
                     GHEPSDIPTANQWLMDNIAPLLHDPKFTTGLVFILTFDEDDSHFGTDNRVYTVISGDR
                     VKTGVNTDVYDHYDLLVTIAGLLNVAPPPLDEPARPIGGIWK"
     gene            complement(7272..8222)
                     /locus_tag="VNN25_23125"
     CDS             complement(7272..8222)
                     /locus_tag="VNN25_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94489.1"
                     /translation="MSKVMVNAHNPMTGPRNLAHLFCIKDYLSSEKQKAYDSFYVIDD
                     VSAFVFYPKSRKKILNRAFFTVRDFLAAGVDPAKSSLLLTSMLPEVFEIGALLNLYVD
                     DRFCEILFAESFTGSLLTYQRQQLGLPRYPSVTEVVHTQTYLAALTIGLDTYKFTGGD
                     EMLGYMPIMEEIVRNFNHENGKTLNLPEMDLVEYPFVLGVDGRHMMSDNAVFLAAPES
                     EIRAQVSKVSENAVLAQWYNAFDQPSVARSLPTSGAPSKEAKKNAGDFLVASLARFRD
                     SKVTNAEILEILRKGAERARPVLAQSLLRIRGALGLPPGS"
     gene            complement(8219..8530)
                     /locus_tag="VNN25_23130"
     CDS             complement(8219..8530)
                     /locus_tag="VNN25_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94490.1"
                     /translation="MAKAKTHTKTSAAAVQAARTAPKLAQWHFATTAPVFDPARNMLS
                     PPMEITSSRIRTLRMIDESVIVHGRPVNVLTKGEDAEDVAVAEKTAKGGRPQAGKERR
                     R"
     gene            complement(8523..9803)
                     /locus_tag="VNN25_23135"
     CDS             complement(8523..9803)
                     /locus_tag="VNN25_23135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH94491.1"
                     /translation="MYVLADYVKYRREGFGGIVLDLPSKTTRFYNVAAAQLLEAASET
                     VSQRELNAFRQGDHQPRGQFLQELLSLKLLRSVSRRTTSLAPMYFTDVASFPESHLFA
                     PLGVELEITLKCYRKCVYCCYESEPLVNTSGELTTAQWCSTLLRLRDNGVFYVRFTGG
                     DPFVRDDFLEILRFADDLGLIVSVGSDLTILEEEHVTALAECTNLLMVQTTLDGATER
                     SADALRGPGNWRKVVRGLRMLAEQGVPVVVGTVLHRGNIGEIGEIAKLVANCGATGYH
                     LAPLYSAGRGRELEHLIPTNADLMIANQAFREVIEAGVVAAADPAWTDVVKGLEAAEL
                     DHAWDDQPYLVRSPDRLLRIDPWGRCYSSIQVKEVVGDAVYVGNMLENSLHDIWHNAP
                     LLSAMRSFETSSTIFGEVVDIRRFAVRLKEAANG"
     gene            complement(10249..>15516)
                     /locus_tag="VNN25_23140"
     CDS             complement(10249..>15516)
                     /locus_tag="VNN25_23140"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HXH94492.1"
                     /translation="EELEAAIREGNVSKHYDNALIMYGRWLERQGRVVIVDDGRAERR
                     PDYTKALIAYRRILSEFANGESRFRDEAKNAVDRITRPTVSVQVSNVFLPDSEVEFAL
                     SWRNVKSIDLSLSSIDLTKDVNLNASPSWLAAITPAPGAQIRNWKKETEDKGDHEPGS
                     DHIRIDPKLPAGAYLLEANGGGTSERALVLITDASIVMRTDMSKVVVFACSALTGAPI
                     ANARLRIWERHGEDGHDVANSFDATTNTDGLAEVSLKQTQYMNLFAAIAQGNGRQAFA
                     TASKGGRYGDQGQWRIYAYSDRPAYRPEETVQWKFTARRFDGETPRNPAGATLHYTIT
                     DPRGTKVADGSVKLNAFGSAWGSLPLTTTMPLGVYEISFRDGQNDVGGAQLFRLEEYK
                     LPEFKVEVHTPEENGKKKLFRLGDTIEASIDASYYYGGPVANAKVEAVVYSRPYTRWW
                     WRPRDYAWLYANSTEPMNYYGNGQVIKRETLTTDASGHAVIHIPTQRDGNEVQYQIEA
                     RVTDASRREITGSGSVRVTRQRYLVHADPDHYVYQPNQKAVIAFKAIDANDNPVPASG
                     TVTITRDWWDESWIDPQGHEVSGAELERVRSTMRIFPPKPHDPCGWQLKSRGYKHEEV
                     LTTKSETDANGEAKVTFTPSRDGYYRVSWSSPDRDPSKPNAPLAQRDVVTTETTVWVV
                     SHDVRDIGYRHDGVEIIVDDAAFRTGQTAPVMIVTPTSNRWVLFSVDAESILDYRLVH
                     VEGTVKLIELPIEEKHVPNAYLNAASVADLALSVDQKEIVVPPLRNVVDVTVKPDREQ
                     YQPREEGTLTVTTKDADGKPLSAEVGLGFVDDSVYAIQSDFAGDPRQFFFGTKRSANV
                     QTAASVQQGRYAKFILDDGRVVDERMVEQPADERLKKDRDDLGREEFDGVVGGVAGGA
                     VAEAITVTAAPADREMAKSMAMPVMAPPPPPSAPKPVVSQGATAEPAVQVRNDFRSTI
                     IWQPDVKTDANGVATIKITYPDSLTTWRVTARAASATNQFGMATSTTRTKKPLIVRLE
                     GPRFFVVGDRVTISAVIDNNTDAPMQVRPSLYHGGLTRIDPLATGQRSLHEDPPVDVP
                     ANGETRVDWLLGVEAPGTASLQVSARGDKFSDAMEKTFPIYDHGIDKLIAKSGRMRGD
                     DAVIKLDLPAERRVGSTNVTIQVTPSLAVTMLDALPYLIDYPYGCTEQTMSRFLPAAI
                     VARTLRGIGADPADIAGRMFGGIEASTASETHPKGKHDFGELDRITSAGMKRLYDFQH
                     DDGGWGWWKEGSSDPYMTAYVIWGFAVAREGGLTIHTQAVDNAANWLDTKLVNYETNR
                     EEQAWLLHALGAWRRTSKAAAASNKLESKALDNVWEHREKLSAYARALFALAEHDFGN
                     AERANILIRNLENGVRIDRAPDASVLIHGENGRTPGSAETMATAHWGEDGMWWRWSDG
                     PVESTSFALMALVEIDPQNKLIEPVTNWLIKNRRGAQWNNTRDTAITILALDEYLHAS
                     GELTSEATYEISANGVPIATQTVKPSEILRAPSRWTIPAPSIRDTINEIHIHRTKGTG
                     SLYFAAEARFFSLEEPIKAAGNEIFVRRDYYHLVGHPTLLKGQVYDKVPLRDGETINS
                     GERVEVVVTVETKNDYEYLLFEDLKPAGLEAVEIQSGQPLYATELKSGAVTRKFVEAK
                     RPAMEKPSVIQRNPNTDTTGRSEWLYQELRDRKVAIFASQLPQGVWEIRYTLRAEVPG
                     FFHALPLMGSAMYVPEIRANSEEVRVGVK"
BASE COUNT         2903 a         4636 c         4809 g         3168 t
ORIGIN      
        1 ccggtatcgg cgatgaagat ctcgcccttg gcgttgacat cgatgccgga tgggctgttg
       61 aggtgtgcgc ttcgcgcgat gccatcgtcg ttgcccgcca cgccaggctc ccccgcaatc
      121 gtcgttaccg ttcggtcggg agcgatgcgt cgaatcgtgt gatttccggt atcggcaacg
      181 aacacgcttc cgtccgggcc agctgcaaga ccgcgaggac tgttgaatcg agcggacgct
      241 ccggcgccat ccagactccc cgcctgccct gcggcgcccg caatcgtttc gaccacccgc
      301 gtctgcggac aaccctcgaa tgagccggtg aatcggatct ggcgaatcgt gtgattgccg
      361 gtatcggcga tgtaaagcct tcgcgttgcg aatgggaacg gatccgcgta gacccaggaa
      421 cgcgccaggc cggttggtgt ccggaattgc gcttcgttcg ccttgctgta cgcgtatccc
      481 gccgtcccgt cgacgctgcc gggcgtattg gcgaggccga tcagcggcga gacatcgtcg
      541 cgcgcagcca ccgaacccgc catcgagacc aatacgagct gttcgctccc actgctcgaa
      601 acgaacatgc cgctgtcgta ttgtcccgat ccacaaccgg aatgcggagg ctccatgacg
      661 atgccccctc caagcggccc gccaaagtcg aacgctacat acgatggccg gtccaggtag
      721 gcgacctgat aggtggaaac gcccgtcgcc gagatccgcc ggatggactg attgccgcgg
      781 tccacgacaa agatatcgcc aggacgggcg ggcgaaccag agacgggagc cacatcgacc
      841 caggtaggcc ggttgaacgt cgccaccgca accggcccat cagtaaaccc cgccaacccc
      901 ggaacgccag ccacggtcgt cacgactccg acaggccggt ccgcagcaaa agcaggggca
      961 acaacaagaa ggagacaaat cacaccgatg gaggcacgca tgatcaagac gcgtgcaaac
     1021 gacgagcctt caacgacgga gtagatcgtc atcgatcgag ctggaccgtg agaggaaatg
     1081 agcctcagtg cgccgcacgc ctgcgcggct ggatgaagaa ttcgatgccg atctccgcgg
     1141 agctggtcgc cgaacctgcg aacgcctgca cccagacgta ggacacgccg aggtcattgc
     1201 cgatgaacgt cagacgggga tcggacgata gtccgagggg gctggagaaa tcgccgatac
     1261 gtccgcgata ccaggcaaag agcggcgcct ggttgttgtc gggaaggagt gcagtgacga
     1321 tcaccggttg cccgagcagg acgcgcgtcg cctgcgcctg gattgcgacc ggatggattg
     1381 gagggagaac cgtgatggtc gacagctcgc cgcagcaccc cgacgcgcgg acgtgcgcga
     1441 cgcccggcgc cacggcagtc acactcacga cgccgttgtc aggaacctga tccggatggc
     1501 aggcagaact gcctgaggca aacccgtgaa cgatggccac cgccggattg tcactgatga
     1561 agtcataacg ataagggaat ccgcaggtga gaccgccagg gtgctgcgag cccgtaatgc
     1621 cggcgttttc accagcgtgc atcgtgatta ctttcggcga gatagacggc cccgcttgcg
     1681 ccgccaaccg aaacgccgcg gcggtagcga gcaggctcag tacgatcgca cgcatcgcac
     1741 aaaggtacga agctgcagcg cgatgtgtcc actccgactg agtgaaacgt ggcgcaggca
     1801 ttcctgcctg ctcccgtcgg gcattcctgc ccggcggtat tttcgaggcg acccacttct
     1861 caggagcatc tatcgtggcg acgccccccg gcaagcctga gctgaggcga caatttggac
     1921 aatcaacacc gccgggcagg gacgcccggc ggtagcaggc aagaatgcct gcactacgat
     1981 cgcggaggcc tcgacaacga cgaagcgata ttgttagcta gtcctgtcgt agatcgcccg
     2041 cattccgtca gggacgatcg agaaagtgga ttcgaatgca acgacgacca tttcgcgctc
     2101 gaatgcgcat cagcgcgata gcccttttgt cgctcgcgat gaccatttcg ggcgttgccc
     2161 agaccgatcc tgcctcgcgc gaccccttcg agacggaaca ttcgttttcg aagagcgtca
     2221 tccatgatct tgacgcgcgt agtttcaagg atctcgatgc gcgcgctgcc gacatccgca
     2281 aaaggcgcgc gatgttcgat tccggcgcgt ggaaactctg gtggttcgtc gacgcattca
     2341 atattcggga gccggcactt cacggcgttg aaccgcaaag tgtcgaaacg ctcatccacg
     2401 aatggatcgc gacgaccggt agcgtcacca gccgggtcgc tctcgcgcaa tttcacctgg
     2461 gattcgccga ttcacttccc cggaatgcct ggcaggatca tgagaagcac gtcgccgcgg
     2521 cgacgcagat tctggactca cttcaggagg agggacgatg cgatatcgac tgtctctatc
     2581 tccagatctg cgcgcacaac ctcggcggat ggagcgaccc ccttcgcaag gtgctgaaga
     2641 cggagccggg gtactggcat gcgttctcgc tcgcaaccta ttttctcgcg ccccgatggg
     2701 gcgggagcac acgggagatc gagaagttcg ccgcggaggc cgccgatgcc acacgcacga
     2761 cgatgggcga atccgtttac atgaaagcga ccgccatggc gctctccact tgcgcctgcg
     2821 agcccacata cgactggaag cgtctgcgca agggatttga ggagtttcaa tctcgatttc
     2881 cgagttcgcg agtcaatttc tatagcttcg ccagacttgc attcgaggcg cacgatcgcg
     2941 aaatgttccg acgactcata gcaaacccct tcatcgagtc ctggcaattt caggaggaga
     3001 gaaaatgggc aatggaacca gacccgccag tctcgtcccg atcaacgaac ctggcgcggg
     3061 tggcccttac cgagctgaca acatcggggc acacggagca tccgatcctg ttctactacg
     3121 agtccggcgg caagaccttc ggagcctcgg tcgccccttc gacgattgac caatggacgt
     3181 ggaaccgccg gcaggtaacc tcgtcagtcc gcgttccgct ggagcagtcc gcgactccga
     3241 gcatcgtctt cgacgcagag ggcgaaacgg ctctgagccc ttttcacatt cggagcgtcg
     3301 agccgattat tctcgagctg gtcacagcaa tcacctgccg ggtcttcgag aacgcctgcg
     3361 tgcccgtgcg ctttcaggca aggatcgtcg gtcaaaacaa cgagaacctc accctccgag
     3421 ttctcgagcc acccgggttc gatcaagcca taggggctcc catcatcgac gaccgcgaat
     3481 cactcctcgg catcgtccac agcgtcacca gaaacgagga cggaagtctg gagctctccg
     3541 cggagccgat ccacttcgta ttgggagtca atcggacaca aactccgatt ggagcgaagt
     3601 agaagcacgc taccggtata tacatagctc ccacaaaaca acagcgcggg cgcgactcgg
     3661 acgcgcccgc gcatcgatca acgcgtgata ccgatcacgt cacttccgtt cgagctcggg
     3721 cggcggttca accgtcgccg ttgctttcgg cggtgccgct ttcggcgctt tggccttgcg
     3781 cgagcgcttg ccgagggcgt tcttcatgtc tgcgacatga ggagcgagat cggccgtctc
     3841 gggttgcttg gccagacgct gcgccagagc gtaggtcgtc agcgcctccc tccctgcctc
     3901 atgcttcgcg ctcgccacgg tgtgacggac gaagctggac attgcatcca gctcatcggc
     3961 gaatggcagg taggcttccg cgaagctcat catgtcgcga accttttcgg gatccgcggc
     4021 acctccacga acgaggctgt tgctgttggc gactgccaca gcggacagtt cgatgaacgg
     4081 cgcgggaagc gaggccacac tggcaatctg ccgcctggcg cgtttggact cagggaacac
     4141 gaaatacggg atcctctccc gcatcgcgcg aacctcaagg accagcgcct gcgctgcagc
     4201 ggtgtgtgaa actggtggat cggtgttgga tacttctttg ggggtagaca tcatttgctc
     4261 cttactctat cgattcaact tgcagtggtt agagggtgcg cgttgcaagg aacggaggag
     4321 cacggcctcg gcagatccgt cttgcaccct cggcagccgt cgagcgggca gaccgttgac
     4381 gccgcttatg caggaattga atgcaactgc tgttgcgcgg caaacgacgc tccggcggac
     4441 ggtgcatggc gatgcgccgc ggagcgggga cgcgctcgtc ccccagcagt tcgtcgagat
     4501 cgagcagcag agcgcctccc gccatcgcct ccaagagtgc gatgtagctg tcatcgcacc
     4561 ggatggagag ggactccatc tttctgaccg cttcgatcca actggaggtg gcagcggcca
     4621 gttcctcggg ggcactcgag ggaagcgatg gcgttgaggc gatcctgccg gccatcacgc
     4681 tcagaccgcg tgcggcgtag gacaagtgcg ttgtgatctc gcgcagccaa tccgaagcga
     4741 gctcaaaatt gcgggcggcg cggaggggac gctccgctgc gaatccctcg gcggcgtcga
     4801 cggcgcggca tgaagcgtcg atcaaccgca gcgaacggcc gatggcccgg agcgtgcggc
     4861 ggaatggacg gccccagaag cggcggcgct ttcgttccgt gccgtaggaa gacactccgg
     4921 ccctccgcga ctgagcggcg ttcctacggg ggcggaaccg ggtggctgcc tttcccaaaa
     4981 caatgcctcg ttgcgtactt agacgtaagg cgtcgagaaa ggtcacagac ctctgctgcg
     5041 ttgctggttt ctatggtgcc taagttgctg gtttctatgg tgtttgggct actgcgtgaa
     5101 gcgcaatcga ggagaccatc agcggcatcc ggggaagcca cggaggccat gggcgatgcc
     5161 atcgacggca tgagtgatgg catcgatggc atgggggggt gccatcgatg gtattgatcg
     5221 ttgccatcga tggcataggg gtttgccatc tatgccatga gtgatgccat cgatggcaag
     5281 ggtggttgcc ttcgatggca tggaacgatg tcatcgatgg tattgctcgt tgccttcgat
     5341 ggcatcgggt gatgccatcg atggtatgga cgatgccttc gatggcatgg gtcgttgcct
     5401 tcgatggttt tggacgatgc catcgatggt attgggcgtt gccatcgatg ccatgggtga
     5461 tgccatcgat ggcatcgagc atggccatca atcgcgtgcc gcggaggcgt gaatcgtgtc
     5521 aggcgcaggc gccggcttct tcgccctcgc tgtcgacggc acgggcgaag tcatcgatgg
     5581 agccgctccc gcgtctcgcg gcctgacccc acgacagcgc agcacaacaa cctgcgcccg
     5641 gcgagtgccg gacgcaaggc gtgatgcacg gatgtcaggg atcctagcgt ttctctttgt
     5701 actcggtctt ggtttcctgc tcgttccccg acgaatcggt cttcgtttcc ttcgtggtgg
     5761 tcttcgtttc cttcggatca ccgaacatgt cgttcttggt cttcgtctct gtctcagtga
     5821 cgcgtttctc gtctgccatt gggtaacctc ctgcatacgc tttcagcaac ggcagtgcca
     5881 gtgcacgtgc ggattcgctg tgaaaacgag gcggtccgaa cggtacggtg atggggtcag
     5941 gtctgaagtg tgaagtaacg aaagcccgat cgaggttctt gcctcacttt cgattcgtta
     6001 cttcacactt cagacctgac cccaccacct acttccagat cccgccgatg ggccgcgcgg
     6061 gctcatcgag cggcggcggg gcgacgttga gaagcccggc gatcgtgacc agcagatcgt
     6121 agtggtcgta gacgtcggtg ttgacgccgg ttttgacgcg atcgccggag atcaccgtgt
     6181 agacccgatt atctgtccca aagtgcgagt cgtcctcgtc gaaggtgaga atgaagacga
     6241 ggccggtggt gaacttcggg tcatgcagca gcggcgctat gttgtccatc agccattggt
     6301 tcgctgtcgg gatgtccgag ggctcgtggc cgtcgtgcgt caagtcgggg atcagaaggg
     6361 cgaagtccgg cagcgcgccg ccagcgatgt cggcgcgcaa cgccgccacc gggtccttcg
     6421 gagtatcgag gcggacgatc tgcgcgcagt cgctcttctg cacgtcaacg aaactgagga
     6481 acgggacgtg ccggcggaca taccccttgc gatgtacccc gctctccttc cccagaaagc
     6541 actggcatcc tcttcacctc aacggcgtga gctccgcgca ggcgcagggg acgcacagca
     6601 gagcgacgag ggcgaatagc ttccgaatcg tcattgcatt ccccgcgcag ggctatcttc
     6661 gcagccaacc gcgtaggcgc tcgctccacg atggcagagc caaggccacc tttgccatcg
     6721 tttcgcgcaa agcgctgtgg actgcggtgg cgcagcccac cgctttgaat cacgtgttga
     6781 aagcggtggc tggcgccacc gcactccaaa gcgcttcgcg cggaatgctg gcgagtactg
     6841 cgcgcacttg gtcagtagcc gagtccggcc cgacttgact ctgctcgatg ccgatacaga
     6901 tgcatcgagg ggtcgcgtac cgaggcgtcg tcactttcgg tttcgacctg acccgccact
     6961 accacaccca gtgtgcgcgc ctcgacgtca gtacaatgat ctgcatgaac gagatcatct
     7021 ttctcgtgga ggaagctccc gaagacggtt tcccgcctgc gcgctcggcg aatcgatctt
     7081 catagaagcg gacgacttcg cttctcgaag gcaacgcgtg atgccgtgcg ttgccacttc
     7141 gaagtgaatg agatgccaca cgttcgtgcg gctccatttc actcgcgaag aggtcatccg
     7201 cctccattga caaggtccgt ctgcaattgc gagaacagcc gtgggtcgga tcgtgagcct
     7261 ggaactcctc gctacgatcc gggtggcagt cctagagcgc cgcgaatccg caataagctc
     7321 tgagcaagaa ctggccttgc cctttccgct cccttcctca gtatctcaag gatctccgcg
     7381 ttcgttacct tagaatcccg gaaacgtgcg agggatgcca ccaagaagtc gccagcgttc
     7441 tttttcgcct ctttgctcgg cgcaccagac gttgggaggc tgcgtgctac tgacggctga
     7501 tcaaaggcgt tgtaccattg cgctagaaca gcgttctccg acaccttgct cacctgtgcc
     7561 cgaatctctg actcgggcgc cgccaagaag accgcattat ccgacatcat gtgccgccca
     7621 tcgacaccga gcacgaaggg atactccacg agatccatct cgggtaggtt gagagtcttt
     7681 ccgttctcat gattgaaatt acgaactatt tcctccatga tgggcatgta gccgagcatc
     7741 tcatcgccac ccgtgaactt atacgtgtcg agaccaatgg tcagcgccgc taggtacgtt
     7801 tgggtatgta cgacctccgt cacagatgga tacctaggta gtcccaattg ctgccgttga
     7861 taggtcaata gactgccggt gaacgactca gcaaacagaa tctcgcaaaa cctatcatcc
     7921 acatacaagt tcagtaacgc accgatctcg aacacctcgg gtagcattga cgtcaacagc
     7981 agggaactct tcgctggatc aacacctgcg gccaagaaat cccgtactgt aaagaaggcc
     8041 cgattgagta tcttctttcg actcttagga taaaacacga aggcggagac atcgtcgatc
     8101 acatagaagg aatcgtacgc cttttgcttc tccgatgaca ggtaatcctt gatgcaaaac
     8161 agatgtgcca agtttcgcgg tcctgtcatc ggattgtgcg cgttcaccat tactttgctc
     8221 atcgccttct ctccttgccg gcctgaggac gaccgccctt ggcggtcttt tccgcgactg
     8281 caacgtcctc ggcgtcttca ccttttgtca agacgttgac cgggcggccg tggacaatca
     8341 cagattcatc gatcatcctt agagtgcgaa tacggctgct cgtgatttcc atcggaggtg
     8401 acagcatatt acgtgcagga tcaaacaccg gcgcggtcgt ggcaaaatgc cattgcgcga
     8461 gtttcggagc agtccttgct gcctgaacgg cggcagcaga agtcttcgtg tgtgtcttgg
     8521 ctttagccat ttgcagcctc ctttaatcgt acggcaaatc gccgaatatc aacaacttct
     8581 ccgaaaatcg tagaggatgt ctcaaacgac ctcatcgccg acagtaacgg tgcgttgtgc
     8641 caaatatcgt gaagagagtt ttccagcata tttcctacgt agaccgcgtc gcccaccacc
     8701 tccttgactt gaatactgct gtagcaccgg ccccaagggt caatgcgaag gagacggtct
     8761 gggctcctga ccagataggg ttgatcgtcc cacgcgtgat ctagttccgc ggcctcaagg
     8821 cctttcacaa cgtcggtcca cgctgggtcg gccgctgcca cgacacccgc ctcgatgacc
     8881 tcgcggaatg cctgattcgc gatcatcaga tccgcgttag taggtataag atgttcaagt
     8941 tcccggcccc gcccggccga gtacagtggg gcaaggtggt acccagttgc accgcagttc
     9001 gcaacaagtt tggcgatttc gcctatttcc ccgatattcc cacggtgcag caccgtgccg
     9061 actacaactg gaactccttg ttcagccagc atcctcagac cacgaaccac cttgcgccag
     9121 tttcctggtc cgcgcagagc gtccgcggac cgctctgttg ctccatcgag agtagtttga
     9181 accatcaaga gatttgtgca ttcagcgagc gcggtaacgt gttcctcttc gaggatcgtc
     9241 aggtccgagc ctaccgacac aatcaaaccc aagtcgtcgg cgaatcgcaa aatctctagg
     9301 aagtcgtcgc gcacaaaagg atcgccgccc gtgaaacgga cgtagaaaac accattgtca
     9361 cgtagtcgaa gcagggttga acaccactgg gctgttgtga gctctcctga agtgttcacc
     9421 aatggttcag actcgtagca gcaatagacg catttacgat aacacttgag tgtgatctca
     9481 agttcaacgc ccagaggggc gaaaagatgt gactccggga agctcgcgac atccgtgaag
     9541 tacatcggag cgagcgaagt agtacgccga ctcacggaac gcagcagttt gagagagagc
     9601 agctcctgga ggaactgtcc tcttggttga tgatcgcctt gcctaaaagc gttcagctcg
     9661 cgctgagaga cagtttcgga ggcagcctca aggagttgag ctgccgctac gttataaaag
     9721 cgagtcgtct tggatggcag atccaatacg attccgccga agccctcgcg acgatactta
     9781 acgtagtccg ctagtacgta catcggttgg aaagtgtaga ccgactattg cgcgttggca
     9841 agttcatctt tgaaaagcag gtgctaaatg ccaaaattag ccgttctatg cgcaggcctt
     9901 tgttcttcga aagcggaaga aaccaacgaa gtgaacctcc gttggctacg tcttgcgttg
     9961 gcaaacaact ataagaagca cgcgtcagcg ctcctcgacg ccgacttcgc gaggccgctt
    10021 ggaaccactg ggaagttggg accacatgct tggcctcctc atcgttgatt gaggagtgcg
    10081 tcccgtgaat tctccccagc cgacaaatcc agaaccaaga agaagcctcc gtgggcgcac
    10141 gcgactaccg tcgggcgaag gcgaaccgaa cgaatgtcgt cgtgcatgac cgcggggcta
    10201 cgggctccct ttcacgctcg atcccggttg aagaacgcct cccccatttc acttcacccc
    10261 caccctcacc tcctcgctat tcgcccgaat ctcgggcacg tacatcgcgg aacccatgag
    10321 tggaagggcg tggaagaagc cggggacctc ggcgcggagg gtgtagcgga tttcccacac
    10381 tccttgcggg agttgggagg cgaagatcgc tactttgcgg tcgcggagtt cctggtagag
    10441 ccattcgctg cggccggtcg tgtcggtgtt ggggttgcgt tggatcactg acggcttttc
    10501 cattgccggg cgtttggctt ccacgaactt tcgggttacg gcgccggatt tcagttcggt
    10561 ggcgtagagc ggttggccgc tttggatctc tactgcttcc aggccggcgg gtttgaggtc
    10621 ttcgaagagg aggtactcgt agtcgttctt ggtttctacc gtcacgacga cttcgacgcg
    10681 ctcgccgctg ttgatggttt cgccatcgcg cagggggact ttgtcgtaga cctgtccctt
    10741 cagcagcgtg gggtggccga cgaggtggta gtagtcgcgg cggacgaaga tctcgttgcc
    10801 ggcggctttg atcggttctt cgagcgagaa gaatctcgcc tcggcggcaa agtagaggga
    10861 tccggtgccc ttggttctgt gaatgtggat ctcgttgatg gtgtcgcgga tcgacggagc
    10921 ggggatcgtc cagcggctgg gcgcgcgcag gatctcggag ggcttcacgg tttgcgtggc
    10981 gatggggaca ccgttggcgc tgatctcgta ggtggcttcg ctggtcagct cgccgctggc
    11041 gtggaggtat tcgtcgaggg ccaggatggt gatggcggtg tctctggtgt tattccattg
    11101 cgcgccgcgg cggttcttga tcagccagtt ggtgaccggc tcgatgagtt tgttctgggg
    11161 atcgatctcg acgagggcca tcagggcgaa tgacgtcgac tcgacggggc cgtcggacca
    11221 gcgccaccac atgccgtcct cgccccagtg agcggtggcc atcgtctcgg cgcttcctgg
    11281 ggtgcggccg ttctcgccgt ggatgagtac cgaggcgtcg ggggcgcggt cgatgcgaac
    11341 gccgttctcg aggtttctga tgaggatgtt ggctcgctcg gcgttgccga aatcatgctc
    11401 ggcgagggcg aagagggcgc gggcgtaggc agagagtttt tcgcgatgct cccagacgtt
    11461 gtcgagcgcc ttggactcga gcttgttcga cgcggcggca gccttggacg ttcgccgcca
    11521 cgcgccgagg gcgtggagga gccaggcttg ctcttcgcgg ttcgtttcgt agttcacgag
    11581 tttcgtgtcg agccagttcg cggcgttatc gacggcttgc gtgtgaatcg tgaggccacc
    11641 ttcgcgggcg acggcgaagc cccagatgac gtaggcggtc atgtagggat cactgctccc
    11701 ttccttccac caaccccagc cgccgtcgtc gtgctggaag tcgtagaggc gcttcatgcc
    11761 ggctgaagtg atgcgatcga gctcgccgaa gtcgtgtttg cctttcgggt gagtctcgga
    11821 ggccgtgctg gcttcgatgc cgccgaacat ccggccggcg atgtcggcgg gatcggcgcc
    11881 gatgccgcgc agcgtgcgcg cgacgatcgc ggcggggagg aagcggctca tcgtctgctc
    11941 ggtgcagccg tagggatagt cgatgaggta cggcagcgcg tcgagcatcg tcacggccag
    12001 actcggcgtg acttgaatgg tgacgttggt cgatccgacg cgtcgctcgg cggggagatc
    12061 gagcttgatc accgcgtcgt cgccgcgcat gcggccggac ttcgcgatca gcttgtcgat
    12121 gccgtggtcg tagatcggga aggtcttctc catggcgtcg gagaacttgt cgccgcgtgc
    12181 gctgacctgg agactggccg tgcccggcgc ttcaacgccg aggagccagt cgacgcgcgt
    12241 ctcgccgttg gcagggacgt cgacgggcgg gtcttcgtgc agcgaccgct ggccggtggc
    12301 caacggatcg atccgcgtaa gcccgccgtg atacagcgac ggccgcactt gcatcggcgc
    12361 gtcggtgttg ttgtcgatga cggcggagat ggtgacgcgg tcgccgacga cgaagaagcg
    12421 cggaccttcg agcctgacga tgagcggctt cttcgtgcgc gtcgtgctcg tggccatacc
    12481 gaactggttc gtcgcggacg ccgcacgcgc cgttacgcgc catgtggtca gcgagtcggg
    12541 ataggtgatc ttgatcgtgg cgacgccgtt cgcgtcggtc ttcacgtcgg gctgccagat
    12601 gatcgtggag cggaagtcgt tgcggacctg aaccgcgggc tcggccgtcg cgccttgact
    12661 cacgaccggc ttcggtgctg acggtggagg cggtggcgcc atcaccggca tagccattga
    12721 tttcgccatt tccctgtccg cgggcgccgc tgttaccgta atcgcctcgg ccaccgcacc
    12781 gccggccact ccaccgacca caccgtcgaa ctcctcacgc cccaggtcat cacggtcttt
    12841 cttcaaacgt tcgtccgcgg gctgttccac catccgctcg tcgacgactc tcccgtcgtc
    12901 gaggatgaat ttcgcgtaac gcccctgctg cacgctggcc gcggtttgaa cattcgcgct
    12961 gcgcttcgtg ccgaagaaga actggcgcgg atcgccggcg aagtcgctct gaatggcgta
    13021 gacggagtcg tcgacaaatc cgagtccgac ctcggcagac aacggcttgc cgtcggcgtc
    13081 cttcgtcgtg acggtgagcg tgccttcttc acgcggctgg tattgctcgc ggtctggctt
    13141 cacggtgaca tcgacgacat tccgcagcgg cggaacgacg atctccttct gatcgacgga
    13201 gagcgccaga tcggcgacac tggccgcgtt gagatacgcg ttcggcacat gcttctcttc
    13261 gatggggagc tcgatcaact tcacagtccc ttcgacgtgc acgaggcgat agtcgaggat
    13321 cgattcggca tcgacgctaa aaaggaccca gcgattgctc gtgggcgtca cgatcatcac
    13381 cggcgcggtc tggccggtgc ggaaggcggc atcgtcgact atgatctcga cgccgtcgtg
    13441 gcgatagccg atgtcgcgga cgtcgtgcga gacaacccag accgtcgtct cggtagtgac
    13501 gacatcgcgc tgcgccagcg gtgcgttggg cttcgacgga tcgcgatcgg gactcgacca
    13561 gctcacgcgg tagtagccgt cgcggctcgg cgtgaacgta accttggcct ccccgttcgc
    13621 gtcggtctcg ctcttcgtcg tcagaacctc ttcgtgcttg tatccgcgcg acttgagctg
    13681 ccagccgcaa ggatcgtgcg gcttcggcgg gaagatgcgc attgtgctgc gcacgcgctc
    13741 gagctccgcg ccgctgactt cgtgaccctg cggatcgatc cacgattcgt cccaccagtc
    13801 gcgcgtgatc gtcaccgtgc cgctggcggg gacgggattg tcattggcat cgatggcctt
    13861 gaaggcaatg accgccttct ggttcggctg gtagacgtag tgatccgggt cggcgtgcac
    13921 gagatagcgc tggcgtgtga cgcgcacgct gccgctgccg gtgatctcgc gccgcgaggc
    13981 gtcggtgacg cgcgcctcga tctgatactg cacttcgttg ccgtcgcgct gcgtcgggat
    14041 atggattacg gcgtgtccgc tcgcgtcggt tgtcagcgtt tcccgtttga tgacctggcc
    14101 gttgccgtag tagttcatcg gctccgtgga gtttgcgtag agccaggcgt agtcgcgcgg
    14161 acgccaccac cagcgcgtgt acgggcgcga gtagacgact gcttctacct tcgcgttcgc
    14221 aaccggaccg ccgtagtagt agctggcgtc gatgcttgct tcgatggtgt cgccgaggcg
    14281 gaagagtttc ttctttccgt tctcctcggg cgtgtgaact tcgaccttga actcggggag
    14341 cttgtactcc tccaggcgga agagctgcgc gcctccgacg tcattctgcc cgtcgcggaa
    14401 cgagatttcg tagacgccga ggggcatggt ggtggtgaga gggagcgagc cccaggcgct
    14461 gccaaaggcg ttcaacttca ccgatccgtc ggcgaccttc gtgccgcgcg gatcggtgat
    14521 cgtgtagtga agcgtggcgc cggcgggatt ccttggcgtc tccccatcga atcgccgcgc
    14581 cgtgaatttc cactgcacgg tttcctcggg gcgataggcg ggtcgatcgc tgtaggcgta
    14641 gatgcgccac tgaccctgat cgccgtatcg tccacccttc gaagccgtgg cgaaggcctg
    14701 ccggccgttg ccttgcgcga tggctgcgaa gaggttcata tactgcgttt gcttgaggct
    14761 cacctcggca agaccatcag tgttggtcgt ggcgtcgaat gaattcgcta catcgtgtcc
    14821 atcttcgccg tggcgctccc agattctgag gcgagcattg gcgatcggcg caccggtcaa
    14881 cgcactgcac gcgaacacga ccaccttcga catatccgtt cgcatgacga tcgaggcgtc
    14941 ggtgatcaga acgagagcac gctcggatgt gccgccgccg ttggcctcca gcaggtacgc
    15001 gccggccgga agcttgggat cgatgcggat gtgatcggag ccgggctcgt gatcgccctt
    15061 gtcttccgtc tcctttttcc agttgcggat ttgcgcgcca ggcgccgggg tgatcgctgc
    15121 gagccagctc ggtgacgcgt tgaggttgac gtctttggtg aggtcgatgg aggagaggct
    15181 gagatcgatc gacttcacgt tcctccacga caacgcaaac tcaacctcgg agtcgggaag
    15241 aaagacgttg gagacctgga ctgagactgt aggcctcgtg attcggtcga cggcgttctt
    15301 cgcctcgtcg cggaagcggc tctcaccgtt cgcgaactcg gagaggatgc ggcgatatgc
    15361 gatcaacgct ttcgtgtagt ccggccggcg ttccgcccgc ccatcgtcaa cgatgaccac
    15421 gcgtccctgc cgctccagcc agcggccata catgatcaag gcgttgtcgt aatgcttgct
    15481 gacgttgcct tcgcggatgg ccgcctcgag ctcttc
//
LOCUS       DATLVW010000789         3991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_367350, whole genome shotgun sequence.
ACCESSION   DATLVW010000789 DATLVW010000000
VERSION     DATLVW010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367350"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VNN25_23145"
     CDS             <1..1088
                     /locus_tag="VNN25_23145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94493.1"
                     /translation="VISFSRQFPKRFYPGGDDIDPTLPRADAAFIVDVMNPFTWLRAG
                     WRLRKYDAVIFVWWVWLFAPAYLVMIAMMRRRTTVILQCHNVGDKEPAAWKRWLTNRV
                     LRRGDALVVHARTEADEAWKRSGGRRVVKTFLPVHELGGAIPSRAEARATLDIDNAAN
                     VALFFGHVRPFKGLDIALCAWRELHTEVILIVAGEAWWERADEYVRLAAELGLAQTQR
                     RSDSQSDKTTSRTGESDLRKTDLRSNVRFDFRFIPDSEIATFFAAADVILAPYRIEAQ
                     SGVALTAFHFARPVIATTVGGLPEVIDGSNGMLVPPDNPTALARAVDEFFTTRDRTSM
                     EQSAAAAARKYSWTEYAAVFAALVDGR"
     gene            1085..2011
                     /locus_tag="VNN25_23150"
     CDS             1085..2011
                     /locus_tag="VNN25_23150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXH94494.1"
                     /translation="MKLNPRRIKVGLELTSKCNLRCGMCPLPVLRRPYEDMVWPMVER
                     AEREIHGAGLKLKWLHEMGEPLLYARIDDAIRLFPEASLSTNGLVLTEEIGAKLLASP
                     LKRIRICVDSIQPDVYPQLRTGGDFDKLVVLTRAFLDQAKGHPIRIEIQKMRSRLTLE
                     ETVDDFRSLFDLKKYRNARVIEKTCEALDVNEETDLHGKFYGCVQGAFFTWVVIFADG
                     RVTHCCYDAHGDQVLGDLKTQSLGEILDSPRFATMQAAFERRDFTELPRCAECFKHGG
                     ESHTFNDVLTRVQNLPGVAKDVVRKVIDVRYR"
     gene            2008..2277
                     /locus_tag="VNN25_23155"
     CDS             2008..2277
                     /locus_tag="VNN25_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94495.1"
                     /translation="MIHLLNIIFGLIPTLILGASIAAGVDDDSSHRRMFLFVYGLWAI
                     TLAMWNWMRSAPPAWTILWLVSGVLTLGYLIARRSMSAPTRKDSL"
     gene            2274..2726
                     /locus_tag="VNN25_23160"
     CDS             2274..2726
                     /locus_tag="VNN25_23160"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH94496.1"
                     /translation="MKKIVLLVSILAAVLLVNAANGGSFSSAPVVDPSTAIRAVLDAQ
                     TAAWNRGDIDAYMDGYARSDDTMFVGTDVTRGWTKVRDRYKAKYDSKAKMGTLVFSDI
                     EFHPMSNNDVVVTGAWKLTRGTDSPHGRTTLIFHRRPEGWRIVYDHSS"
     gene            complement(2812..3792)
                     /locus_tag="VNN25_23165"
     CDS             complement(2812..3792)
                     /locus_tag="VNN25_23165"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HXH94497.1"
                     /translation="MNPVQPITVAQYGIGPIGAEIARLLLTKPWVRIVAAVDVDPNKI
                     GKDLGEVIGLDREVGVKVTAELTAKPDVVCHSTGSRLRDVSGQLKDLLNRGCHVVSTC
                     EELSFPLDQAIREELQQTARARNVTLLGTGVNPGFVMDKLPLTITSVCQRVDSVEIIR
                     IQNASTRREPLQRKVGAGMTPDEFRAAVAAGKIKHMGLRESLMMVGNGLGAEFESVSD
                     EVIEPIIAEREVKTQYLTCAAGQVAGVHQTIFGKGKINVSLELRMYVGAEDVAADKVI
                     VHGVPDVEMVIKGGVHGDRATAAMVVNSIPRVVNARPGVLTMDDIPISFR"
BASE COUNT          734 a         1252 c         1251 g          754 t
ORIGIN      
        1 acgtcatctc cttctcgcgc cagttcccga agcgctttta tccgggcggc gatgacatcg
       61 atccgacgct gccacgcgcg gacgccgcat tcatcgtcga cgtcatgaac ccgttcacgt
      121 ggctacgcgc gggatggcgc ttgcggaagt acgacgccgt gatctttgtc tggtgggtgt
      181 ggctcttcgc gcccgcgtat ctggtgatga tcgccatgat gcggcggcgg acgacggtga
      241 tcctgcagtg ccacaacgtc ggcgacaaag agcccgcggc atggaagcga tggctgacga
      301 atcgggtgct gcgacgcggg gatgcgctcg ttgtccacgc ccggacggaa gcggacgaag
      361 catggaagcg ctcgggcggg cggcgcgtgg tgaagacgtt cctgccggtt cacgagctgg
      421 gcggcgcgat cccttcgcgt gcggaggcgc gcgcgacgct tgacatcgac aacgccgcga
      481 acgtcgcgct cttcttcggc catgtacgtc cgttcaaggg gctcgacatc gccttgtgcg
      541 cctggcgtga gctgcatacc gaggtgatcc tcatcgttgc cggcgaggcg tggtgggagc
      601 gcgcggatga gtacgtgcga ttggcggcag agttaggcct cgcccaaacg caacgtaggt
      661 cggactctca gtccgacaaa acgaccagtc ggactggaga gtccgaccta cgtaaaaccg
      721 atcttcgcag caatgttcgc ttcgacttcc gcttcatccc ggactccgag atcgccacct
      781 tcttcgcggc cgcggacgtc attctcgcgc cgtatcgcat cgaagcgcag agcggtgtgg
      841 cgctgactgc gttccacttc gcgcggccgg tcatcgcgac gacggtcggc gggttacccg
      901 aggtcatcga cggatcgaat ggcatgctag tgcctcccga caatccaacg gcactcgcgc
      961 gcgccgtcga cgaattcttc accacgcgcg accgcacatc gatggagcaa agcgccgcgg
     1021 cggcagcgcg caagtactcg tggacggaat acgccgcggt gttcgctgcc ttagtggacg
     1081 ggagatgaaa ctcaaccctc gccgcatcaa agtcggcctc gagctgacct cgaagtgcaa
     1141 cctccgctgc ggcatgtgcc cgctgcccgt gttgcgccgt ccgtacgagg acatggtgtg
     1201 gccgatggta gagagggccg agcgcgagat ccacggcgcg gggctgaagc tgaaatggct
     1261 gcacgagatg ggcgagccgc tgctctacgc gcggatcgat gacgccatcc gcctcttccc
     1321 cgaggccagc ttgtcgacga acgggttagt gttgacggaa gagatcggcg cgaaattgtt
     1381 ggcgtcgccg ctgaaacgca tccgcatctg cgtcgattcg atccaaccgg atgtttatcc
     1441 gcaactgcgc accgggggag acttcgacaa gctcgtcgtc ctcacgcgtg cgtttctcga
     1501 tcaagccaaa ggacatccca tccgcatcga gattcagaag atgcgctcgc ggctgacgct
     1561 cgaagagacc gtcgacgact tccgctcgct cttcgatttg aagaagtatc gcaacgcgcg
     1621 cgtcatcgag aaaacgtgcg aagcgctcga cgtcaacgaa gagaccgatc tgcacgggaa
     1681 gttctacggc tgcgtgcagg gcgcgttctt cacctgggtc gtgatcttcg cggacggccg
     1741 cgtcacgcac tgctgctacg acgcccacgg cgaccaggtc ctcggcgatc tgaagaccca
     1801 atcgcttggc gagatcctcg attcgccgcg cttcgccacg atgcaggccg cgttcgagcg
     1861 gcgcgacttc acggagctgc cgcgctgcgc ggagtgcttc aagcacggcg gcgagtcgca
     1921 cacgttcaac gatgtgctga cgcgcgtgca gaacctgccg ggcgtggcca aggatgtcgt
     1981 ccgcaaggtg atcgacgtgc ggtaccgatg atccatctcc tcaacatcat cttcggtctc
     2041 atccccacgc tcatcctcgg cgcgagcatc gccgcaggcg tcgacgacga ctccagtcac
     2101 cgcaggatgt tcctgttcgt atatggtcta tgggcgatca cgctggcgat gtggaactgg
     2161 atgcgttcgg cgcctcccgc atggaccatc ctctggctcg tctccggtgt cctcaccctc
     2221 ggctacctga tcgcgcgacg ctccatgagc gcgcccacta gaaaggactc actatgaaga
     2281 agatcgtctt acttgtttct atcctcgccg ccgtgctcct cgttaacgcg gccaacggcg
     2341 ggagtttctc gtccgcaccg gtcgtcgatc cgtctaccgc tattcgcgcc gtgctcgacg
     2401 cgcaaacagc cgcctggaat cgcggcgaca tcgacgccta catggacggc tacgcccgca
     2461 gcgacgacac gatgttcgtc ggtaccgacg tcacgcgcgg ctggacgaaa gtgcgcgacc
     2521 gttacaaagc gaagtacgac tcgaaggcga agatgggcac gctcgtcttc tcggacatcg
     2581 agttccaccc gatgagcaac aacgacgtgg tcgtcaccgg cgcctggaaa ctgacacgcg
     2641 gtaccgactc gccgcatgga agaaccacgc tgatcttcca tcgccgcccg gaaggctggc
     2701 gcatcgtgta cgaccattcg tcgtgagaat gctctgaaga cgtgcaccac aaagacacaa
     2761 agagcacaaa gaaagacttg gtgtcctctg tgtctttgtg gttaagagcc tttaccggaa
     2821 gctaatcgga atatcgtcca tcgtcagcac gcccgggcgc gcgttcacga cacgcgggat
     2881 cgagttgacg accatcgcgg cggtagctcg gtcgccgtgg acgccgcctt tgatcaccat
     2941 ctccacgtcg ggcacgccgt gcacgatcac cttgtccgcg gccacatcct cggcgccgac
     3001 gtacatgcgc agctccaggc tcacgttgat cttccccttg ccgaagatcg tctggtggac
     3061 gccagcgacc tgtccggccg cgcacgttag gtactgcgtc ttcacttcgc gctcggcgat
     3121 gatcggctcg atcacctcgt cgcttaccga ttcgaactcg gcgccgagtc cgttgccgac
     3181 catcatcagc gactcgcgca ggcccatgtg cttgatcttg cccgcggcca cggcggcgcg
     3241 gaactcatcc ggcgtcatgc ctgcgccgac tttgcgctgc agcggttcgc gccgcgtcga
     3301 tgcgttctga atgcggatga tctcgacgga gtcgacgcgc tggcagaccg aggtgatcgt
     3361 gagcggcagc ttgtccatca cgaagccggg attgacgcct gtgccgagga gcgtgacgtt
     3421 gcgggcccgg gccgtctgtt gcagctcttc gcgaatggcc tggtcaagcg ggaaggagag
     3481 ctcctcgcag gtggagacga cgtggcagcc gcggttgagg aggtccttga gctgaccgga
     3541 tacatcgcga aggcgcgagc cggtggagtg gcagacgacg tcgggcttcg ccgtgagctc
     3601 ggcagtgact ttgacgccga cctcgcgatc gagaccgatc acttcgccaa ggtctttgcc
     3661 gatcttgttc ggatcgacgt cgaccgcagc gacgatgcgc acccacggct tggtcaggag
     3721 taaacgagcg atctccgcgc cgatcggacc gattccgtac tgcgcgactg tgatgggttg
     3781 aacaggattc atgagcacct cgtgagcaaa agtgcgcgga ttgtagtgga tgagggatgc
     3841 gattgctgtc agaactgcag ctaccgttgc cgatgcgcga aacgtgtttg tgtcatcctg
     3901 agccgccgaa gccgccgcaa gattcgctga cgttgcatcc ttcatagata ggcggcgtag
     3961 gacggtcgaa ggatctcaaa atgcgacgat c
//
LOCUS       DATLVW010000790        10751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_529819, whole genome shotgun sequence.
ACCESSION   DATLVW010000790 DATLVW010000000
VERSION     DATLVW010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529819"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..437
                     /locus_tag="VNN25_23170"
     CDS             234..437
                     /locus_tag="VNN25_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94498.1"
                     /translation="MEKLDIAVRDLDIAVRNLDIDVDDLDIDVSSLVINVSYAGIEVA
                     DVAGHDEIQHSEQHRILRWGSSG"
     gene            710..2131
                     /locus_tag="VNN25_23175"
     CDS             710..2131
                     /locus_tag="VNN25_23175"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH94499.1"
                     /translation="MLKQTLIPITALLLAACASVRDIETVTNPSVHGVGTPAAPSTQW
                     TPPAKAIPPPVPAPNDLALPPAGAPLELAQIVGLALSNNPLTRTTWLNARVSEANLGS
                     ARAAYLPAVDLDASLARTHTTTGTATTLTAPNLSLDYLLFDFGGRNAETEAARQMLIA
                     ADYTHNQAIQDVILRVEQAYYDYLDAKALLTAQASTIKERQTSLDSAEARHRAGVATI
                     ADELQARTALSQAQLTYETIDGNLHKLRGTIATAMGLPATTPFEAGELPLDIPAQQVS
                     DAADALIAQAVTERPDLAAARAAIEEARAHVRVVRSQGLPTLGLGANVAPVFANGLQR
                     QITSYSAGVSLRFPLFTGFRNTYDIRAAELETKLASENARGLEQQIGLQVWTSYYDLQ
                     TAVKRLKTSRDLLHDAQESADVAQGRYRAGIGSILDLLTAESALENARAEEVQARADW
                     FLTVAQLAHDTGTLTPTGAPQGK"
     gene            2137..3243
                     /locus_tag="VNN25_23180"
     CDS             2137..3243
                     /locus_tag="VNN25_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH94500.1"
                     /translation="MISMKLTGIRAITAAAIVATLLGCGEKKEKPAEERVPVTVALAE
                     QRDVPVDVRVIGSVQPISSVAVRALVAGQLQRVWFREGDDVRKGQRLFTIDERPYQSA
                     LAQAEANLNRDEAQAKNAEAEAKRYAELVKKDYVTREDYDKFVSGAEAARAVVAADRA
                     AADNARLQLAYCDIRSPLDGRTGSLQVQAGNLVKANDTTPLVAINQITPVYVTFSVPE
                     SQLGDVRARGLGNVPVTASPQQGGGAVQNGKLTFVDNAVDAQTGTITLKATFTNEGRV
                     LWPGEFVNVAMTLSNRRNATVVPAQAVQNGQKGQYVYVVTEGNGIQMRPVTVIQQVDD
                     QAVIGKGVNAGDTVVTDGQIRLTPKSKVEVKKSL"
     gene            3277..6438
                     /locus_tag="VNN25_23185"
     CDS             3277..6438
                     /locus_tag="VNN25_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH94501.1"
                     /translation="MNIAAIFIRRPVMTTLVMLAIVLFGIMSYRLLPVSDLPNVDFPT
                     INVNASLPGASPETMAASVATPLERQFSTIAGLDSMVSSSSLGSTSITLQFALNRNLD
                     AAAQDVQAAIAAAGRQLPPNMPAPPSYNKVNPADTPVLYLAITSENMPLYQLDEYAET
                     MMALRISAIEGVAQVQVFGAQKYAVRTQLDPRQLASRGVGIDEVANAIRNNNVNLPGG
                     VLTGPFTNYTVQANGQLYKAADYAKIIVAYRNGRPVRLGELGTVRDDVENNQTMAWFR
                     GKPGFILAVQKQPGTNAVEVSDRVRALLPTFRSQIPASVDLQVHRDRADSIRASVDDV
                     KFTLLLTLFLVILVIFLFLRNVSATIIPSLALPLSIVGTFAVMYLFDYSLDNLSLMAL
                     TLSVGFVVDDAIVMLENIVRHMEMGEKPMEAAFNGSREIGFTIISMTISLTAVFIPIL
                     FMGGILGRLFHEFAVTIGSAILVSGFVSLTLTPLLSAKFLRAHKGEKHGRFYQATEKM
                     FDGMLSLYESGLRFALRQRAMTLMLSLAILAATVWLFIVIPKGFIPNEDQNEVRISLE
                     AAQGTSFPELVRHQRMAMDIVNQDNRVAAFFSFVGRGGANNSSVISLRLVPKSERKAS
                     IDDVMNDLRPKLNAIPGFRVSLQNPPPIRLGGRQSNSQYQYTLQSADTATLYDASQKM
                     MEKMRTLPGFVDVSSDLFLRNPTVNVEINRDRAATLGLTPNQVEDALFSSYATRQVSQ
                     IYAPNNTYQVIMELDPEYMKNPQALSMLYVRSSSGELVPLDAVAKLIPTVGPLAVNHS
                     GQAPSVTLSFNLKPGTSLGAAVTEINDAAKVTLPATVTTSFQGTAQAFQSSLQGLGLL
                     LLIAIAVIYMVLGILYESFIHPLTILSALPFAGFGALLTLLVFKVDLSIYAFVGIIML
                     VGLVKKNGIMMVDFALEAQKEGKSSVDAIYEACVVRFRPIMMTTMAALMGTIPIALGF
                     GAGAESRRPLGLAVVGGLLFSQTLTLFVTPVFYVYMDKLRKKPSGEVPSDGTTIEDEF
                     AALAPEEQPVGVMFNTVKPEPAL"
     gene            6452..8425
                     /locus_tag="VNN25_23190"
     CDS             6452..8425
                     /locus_tag="VNN25_23190"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HXH94502.1"
                     /translation="MRNQLAVLAFCAAVFTALSAEAQSADLVISKSGTESASAGDTIA
                     YSIFVFNNGPDAASNATVTDSLPAGTTFVSLDASDGTFTCSTPAVGSGGTVTCSAATM
                     PVEGTVRFTLSVKTSSAAPSGSITNTATITSPTPDPDTSSNSSSASTGIVGSSAASAD
                     LSIDSMDRAAAVSAGATASFQVAISNKGPSTANHPHLTNAVPANTTFLSASVADPIGD
                     FHCTTPAVGTTGNIVCTAPKFDVRGGDQPVFTFTFRVNDGVSQETTLTNSATIAADET
                     DPNTSNNTASRTTAITTTAPSADVSVAVSGGGTTFGIVVRNAGPNDAASVTLTDSVPP
                     GSTFVSWSQNSGPAFNCSTPAPGATGTINCTIGVLPGLEGKTTAAEFVLTVDAATQVT
                     NTASVSSSTSDPRPDNNTSSFPVLTKISINDVIVTEGNSGTTPAVFTVSLQPANATLT
                     TTVDYQTTNGTATAGSDFVATSGTLIFLAGETAKTITVPVIGDSIVEGTEFFNVQLSN
                     AVNAAFQDTAGTGTIIDDDLGGPPAPPSVQIGNLSFPEGNSGTSNVTFTATLSAASTN
                     VSRVRWQTQDGTATAGSDYVASSGELVFQPGELTKTFTVAIIGDTNFEPDETFSILIV
                     STDNTSTAPGPAAVATIVNDDVQVPPRHRAVGH"
     gene            8588..8875
                     /locus_tag="VNN25_23195"
     CDS             8588..8875
                     /locus_tag="VNN25_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXH94503.1"
                     /translation="MKLRPLYDRIVVTRKETGEQVRGGIIIPDTAKEKPQQAEVIAVG
                     DGKFSEDGKRMKLDVKKGDTVLIGKYSGTDIKIDDVEYTILREDEVLAVVE"
     gene            8903..10549
                     /gene="groL"
                     /locus_tag="VNN25_23200"
     CDS             8903..10549
                     /gene="groL"
                     /locus_tag="VNN25_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786183.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXH94504.1"
                     /translation="MAKRITYSEDARQAILRGVNKLADAVKVTLGPRGRNVVIEKKFG
                     SPTITKDGVTVAKEIELKDEMENAGAQMVREVASKTSDMAGDGTTTATVLAQAIYREG
                     IKNVTAGANPMDVKRGIEIAVRAAVDSIDKLAKPVEGKDIAHIGTISANSDDEIGSII
                     AQAMEKVGKDGVITVEEARGLDTVLEVVEGMQFDRGYLSPYFVTDPERMESVLENAKI
                     LIYEKKVSSMKDLLPILEQTARQGKPLLIIAEDVEGEALATLVVNKLRGTLNVAAVKA
                     PGFGDRRKAMLEDIAILTGGKLISEDIGVKLENVKWEDLGDAKRVTIDKDNTTLVTDT
                     DDKSRKDQIAGRVKQIRGLIDDTTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKE
                     KKARVEDAMHATKAAVEEGIVPGGGVALLRAIAAVEAVKAEGDVKVGVNVIRRALEEP
                     FRQIVFNAGKEAAVLLNEIKTKGGNWGYDARNDEVVDMISAGIIDPAKVTKQALQNAS
                     SIAGLMLTTEALISEIKEEEKGGGGGNPGAGMGGGMGGMY"
BASE COUNT         2253 a         3503 c         3098 g         1897 t
ORIGIN      
        1 aagtcgagaa tcgagactgc gaggtcgaga atcgagactg cgaagtcgag aatcgagcct
       61 gcgaggtcga gaatcgagac tgcgaagtcg agaatcgagc ctgcgaggtc gagaatcgag
      121 cctgcgaggt cgagaatcga cactgcgatg tcgtcagccg agacattgat gtggaaaatc
      181 tggacgtcga tgcgtcaaat ctggacattg aagtgtcgaa ttgagactgc gatgtggaaa
      241 agctggacat cgctgttaga gatctggaca tcgctgttag aaatctggac atcgacgtcg
      301 acgatcttga catcgatgtc tcgagtctcg tcatcaatgt ctcgtacgcc ggcattgaag
      361 tagccgacgt agccggccat gacgagattc agcacagcga gcagcaccgg atccttcggt
      421 ggggttcctc cggatagacg atgtcgcctc cgccccgagg cgcaggtggt gtgctgccgc
      481 tcgaaggcgg cggtggaggt ggtggagtgt aggaatcagg tagtattagt cggtttcggc
      541 cggtcgcgcc ttctgatcac caacccacgc ttcggattgg actcgctgcc ctgatccact
      601 aatctctgat cacgcagtcc actgctctcc tgatctctga ttcaccccct tcaacgctac
      661 agctttgtaa ccgtagcaag tcctgcatct gttggaatcc acttcggtaa tgctcaaaca
      721 aacactcatc ccgattacgg cccttcttct cgccgcgtgt gcgagcgtac gtgacatcga
      781 aaccgtcacc aatccttctg ttcacggcgt cgggactccc gccgctccct ctacgcaatg
      841 gacgcccccg gcgaaggcga ttcctccgcc cgtgccggca ccgaacgatc tcgccctccc
      901 gccggccggc gcgccgctgg agttggcgca aatcgtcggt ctggcgctat cgaacaatcc
      961 gctgacgagg acgacctggc tcaacgcgcg cgtctcggag gcaaacctcg gcagcgctcg
     1021 cgcggcgtat ctgccagccg tcgatctcga cgcttcgctg gctcgcacac acaccacgac
     1081 cggcacagcc accacgctca cggcgccgaa tctgtcgctc gactatctcc tcttcgattt
     1141 cggaggccga aacgcggaga ccgaggcggc gcggcagatg ctcatcgctg cggactacac
     1201 gcacaaccag gccatccagg acgtcatcct ccgcgtcgag caggcctact acgactacct
     1261 cgatgccaaa gcgctgctca cggcgcaggc atcgacgatc aaggaacggc agaccagcct
     1321 cgattcggct gaggccaggc atcgcgccgg cgtcgcaacg atcgccgatg aactgcaggc
     1381 caggactgcc ctctcgcagg cacaactgac ttacgaaacc atcgacggga atcttcacaa
     1441 gctgcgcggc acgatcgcca cggcgatggg attgccggcg acgacgccct tcgaagccgg
     1501 cgagcttccc ctcgacattc ccgcgcagca ggtcagtgat gccgccgacg cactgatcgc
     1561 gcaggcagtc accgagcgtc ccgatctcgc ggcagcacgc gccgcgatcg aggaggcgcg
     1621 cgcacacgtg agagtcgtgc gttcgcaggg attgccgacg ttaggtcttg gcgccaatgt
     1681 cgcgccggtt ttcgcgaatg ggctgcaacg gcagataacg tcgtactcgg cgggtgtttc
     1741 ccttcgcttc ccgctcttca caggctttcg caacacgtac gacattcgcg cggccgagct
     1801 ggaaacaaaa ctcgcttccg agaacgcgcg cggtctcgaa cagcagatcg gccttcaggt
     1861 ctggacgagt tattacgatc tgcagacggc ggtgaagcga ttgaagacca gccgcgatct
     1921 tctgcacgac gcgcaggaat ccgccgacgt cgctcagggc cgctaccgcg ccggtatcgg
     1981 aagcatcctc gacctgctca ccgccgagtc cgcactggaa aatgcgcggg ctgaggaagt
     2041 tcaggcacgc gcggattggt tcctcacagt tgcacagctc gcacacgaca ctggcacgct
     2101 cacaccgaca ggcgcgccgc aaggaaaatg atcacgatga tctcaatgaa gttgaccggc
     2161 attcgtgcca tcaccgcggc cgcgatcgta gccacgctcc tcggttgcgg tgagaaaaaa
     2221 gagaaaccgg cagaggagcg cgttcccgtc accgttgccc tcgccgaaca acgagacgtc
     2281 ccggtggatg tgcgcgtcat tggcagcgtg caaccgatct ccagcgttgc cgttcgcgcg
     2341 ctcgtggctg gccagctcca gcgcgtctgg tttcgcgaag gcgacgacgt ccgcaaaggc
     2401 cagcgcctct tcaccatcga tgagaggcca tatcagtccg cgctggcgca ggcggaagcg
     2461 aacctgaatc gcgatgaggc ccaggcgaag aatgccgaag ccgaggccaa gcgctacgcg
     2521 gagctcgtga aaaaggacta cgtcacccgc gaggactacg acaaattcgt atccggcgcc
     2581 gaggccgcgc gcgcagtcgt tgccgcggac cgcgctgcgg ccgataacgc ccgtctccaa
     2641 ctcgcctact gcgacattcg ctcgccactg gatggccgaa ccggcagcct ccaggtgcag
     2701 gccggcaatc tcgtcaaggc gaacgacacg acgccgctgg tcgcgatcaa ccagatcacg
     2761 ccggtgtatg tcacgttctc cgttccggaa tcgcagcttg gcgacgtgcg cgcccgaggg
     2821 ctcggcaatg tgcccgtaac cgcttcgccg cagcagggcg gcggcgcagt tcaaaatggg
     2881 aagctgacat tcgtcgacaa cgcagtcgac gcgcaaaccg gaacgatcac gctcaaagcg
     2941 acgttcacga acgagggccg ggtgctctgg cccggcgagt tcgtgaatgt ggccatgacg
     3001 ctgtcgaacc ggcgcaacgc cacggtcgtg ccagcgcaag ccgtgcagaa cggtcagaag
     3061 gggcagtacg tatatgtcgt caccgagggc aacggaattc agatgcgtcc cgtgaccgtc
     3121 attcagcaag ttgatgacca ggccgtcatc ggtaagggcg tcaatgccgg cgacaccgtc
     3181 gtcaccgacg gccagatccg actgactccg aagagcaaag tggaagtgaa gaagtcgttg
     3241 tagcttgggc aacccgatca ccgagccacc cacaccatga acatcgccgc catcttcatc
     3301 cgccgtcccg tcatgacgac gttggtcatg cttgccatcg tgctgttcgg aatcatgtct
     3361 taccggttgt tgccggtcag cgatctgccg aacgtcgact tcccgaccat caatgtgaac
     3421 gcgtcgctgc cgggagcaag ccctgagacg atggccgcct cggtggcgac gccgctggaa
     3481 cggcagttct cgacgatcgc cggcctcgac tcgatggtct cgtcgtcatc gctcggatcg
     3541 acttcgatca cgctgcaatt tgcactgaac cgcaatctcg acgccgccgc tcaggacgtg
     3601 caggcggcca tcgccgcggc cggccgccaa ttgccgccga atatgccggc gccgccgtcg
     3661 tacaacaagg tcaatcccgc ggacacgccc gtcctctatc tcgccatcac atccgagaac
     3721 atgccgctgt accagctcga cgaatacgca gagacgatga tggcgctccg catctccgcg
     3781 atcgaagggg tggcgcaggt ccaggtcttc ggcgcacaaa agtacgccgt tcgcacgcag
     3841 ctcgatccac gccagctcgc cagccgcggc gtcggcatcg acgaggtggc gaacgccatc
     3901 cgcaacaaca acgtgaacct gcccggcggc gtgctcacgg gtccattcac gaactacacg
     3961 gtgcaggcga acgggcagct ctacaaggcc gccgactacg cgaagatcat cgtcgcctac
     4021 cgcaacggcc ggccggtacg cctcggtgag ctaggcaccg tgcgcgacga cgtcgagaac
     4081 aaccagacca tggcctggtt ccgcggcaag cccggcttca ttctggcggt gcagaaacag
     4141 ccgggaacga atgctgtcga agtatccgac cgcgttcgcg cgctgttgcc gacattccgc
     4201 agtcagatcc ccgcctcggt cgatctgcag gtgcatcgtg accgcgccga ctccatccgg
     4261 gcttcggtcg atgacgtcaa gttcacgctg ttgctcaccc tcttcctcgt catcctggtc
     4321 atcttcctct tcctgcgaaa cgtctcggcc acgatcatcc ccagtctggc gctaccgctc
     4381 tcgatcgtcg gcacgttcgc cgtgatgtac ctgttcgatt actcgctcga taacctctcc
     4441 ctgatggcgc ttacgctgtc cgtcggcttc gtcgtcgacg atgccatcgt catgctggag
     4501 aacatcgtcc ggcacatgga gatgggcgag aagccgatgg aagcggcgtt caatggatcg
     4561 agggagatcg gcttcacgat catctccatg acgatctcgc tgactgccgt cttcatcccc
     4621 atcctcttca tgggcggcat cctcggacgt ctcttccacg agttcgccgt gacgatcgga
     4681 tcagcgatcc tcgtttccgg cttcgtctcg ctcacgctca cacccctgct cagcgcgaag
     4741 ttcctgcgcg cacacaaagg ggagaagcac ggcaggttct accaggccac ggaaaagatg
     4801 ttcgacggca tgctctcgct ctacgagagc ggcctccgct tcgcgttgcg ccagcgcgcc
     4861 atgaccttga tgttgtcgct ggccatcctc gccgcgacgg tctggctctt cattgtcatt
     4921 ccgaaggggt tcatcccaaa cgaagaccag aatgaagtgc gcatcagcct cgaggcagcg
     4981 cagggaacat cgttccccga gctcgtccgg caccagcgca tggctatgga tatcgtgaac
     5041 caggacaatc gggtggcggc gttcttttcg ttcgtcggcc gcggcggcgc caacaacagc
     5101 agcgtcatct ccctgcgcct cgtccctaaa tcggaacgta aggcctcgat cgatgacgtg
     5161 atgaacgatt tgcgccccaa gctgaacgcc atccctggct tccgcgtctc gctgcagaac
     5221 cctccaccga tccgcctcgg cggacggcag tcaaactcgc agtatcagta cacgcttcag
     5281 tccgcggaca cggcaacgct ctacgacgca tcgcaaaaga tgatggagaa gatgcgcaca
     5341 ctgcccggat tcgtcgacgt gagcagcgat ctcttcctgc gcaatccgac ggtaaacgtt
     5401 gagatcaacc gcgaccgtgc cgcaacgctc ggcctgacgc cgaaccaggt cgaagacgcg
     5461 ctcttctcat cgtatgcaac gagacaggtg tcgcagatct acgcacccaa caacacgtat
     5521 caggtcatca tggagctcga tcccgagtac atgaaaaatc cgcaggcgtt gtcgatgctg
     5581 tacgtccgct catcttcggg cgagctcgtg ccgctcgatg ccgtcgcgaa actcatcccg
     5641 acggttggtc cgcttgcggt caatcactcc ggtcaggcac cgtccgtgac gctgtcattc
     5701 aacctcaaac cgggtacgtc gctcggcgcc gccgtgaccg agatcaatga cgctgcgaaa
     5761 gtcacactgc ccgcaaccgt tacgacgtcc ttccagggca ccgcacaagc tttccagtcg
     5821 tcgctgcagg gtctcggcct gttgctcctc atcgccatcg ccgtcatcta catggtgctg
     5881 ggcatcctct acgaaagctt catccatccg ctgacgattc tctcggctct gccatttgcc
     5941 ggtttcggcg cgctcctgac gctgctcgtg ttcaaagtcg acctgtcgat ctacgccttc
     6001 gtcggcatca tcatgctcgt cggcctcgtg aagaagaacg gcatcatgat ggtcgacttc
     6061 gccctcgaag cgcagaagga gggaaagagc tcggttgacg ccatctacga agcatgcgtc
     6121 gttcgtttcc ggcccatcat gatgacgacg atggccgcgc tgatgggcac aatcccaatc
     6181 gcgctcggct tcggtgccgg cgccgaatca cgccgcccgc tcggactggc cgtagtcggc
     6241 ggtctcctct tctcgcagac gcttactctc ttcgtgacac ctgtcttcta tgtctacatg
     6301 gacaagctgc gcaagaaacc gagcggcgaa gttcccagcg atggcacgac aatcgaagac
     6361 gagtttgctg cgctcgcacc agaagaacag ccggtgggcg tgatgttcaa cacagtcaaa
     6421 ccggaacctg cgctataaac cttggtacta catgcgcaat caactcgcag tcctggcctt
     6481 ctgtgcggcc gtcttcaccg ccttgtccgc ggaggcacag agcgccgacc tcgtgatctc
     6541 caagagcggt actgagtccg cgtcggccgg cgacacgatc gcgtactcga tcttcgtctt
     6601 caacaacggc cctgatgcag catcgaacgc gacggtgact gactccctgc cggccggtac
     6661 cactttcgtc tcgctcgatg cctcggatgg cacgttcacg tgcagtacgc cggctgtggg
     6721 atccggtgga acggttacct gttccgccgc gacaatgccg gtagaaggga cagtccgctt
     6781 cacactgtcc gtgaagacgt cgtccgcagc acccagcggc tcgatcacca acaccgccac
     6841 gatcacctcc cccacgcccg atccggacac gtcgagcaac agcagcagcg cctccaccgg
     6901 catcgtcggc tcgtcggctg cctccgccga tctctcgatc gactcaatgg atcgagcggc
     6961 agccgtcagc gccggcgcga cggcaagttt tcaggtcgcc atctccaaca aaggcccgtc
     7021 caccgcgaat caccctcacc tcaccaatgc tgttcccgca aatacaacgt tcctttccgc
     7081 cagcgttgcc gacccgatcg gtgatttcca ctgcaccact cccgcggtcg gcacgaccgg
     7141 caacatcgtt tgcacggcac cgaagttcga tgtgcgcggc ggcgaccagc cggtgttcac
     7201 gttcacgttc cgcgtcaacg atggtgtttc gcaagagacg acgctgacga acagcgcgac
     7261 catcgcggcc gatgaaactg atccgaacac ttcgaataac acggccagtc ggacgacggc
     7321 gatcacgact acagcgccat cggccgatgt ctccgtggcc gtatccggcg gaggcacaac
     7381 cttcggcatc gtcgtacgca acgcgggtcc gaacgatgcc gcgagcgtga ccttgaccga
     7441 ctccgtcccc ccgggcagca ccttcgtcag ttggtcgcaa aacagcggac cggccttcaa
     7501 ctgcagcacc cccgccccag gcgcaaccgg aaccatcaac tgcacgattg gcgtcctgcc
     7561 cgggctcgaa ggaaagacga ccgctgccga gttcgtactc acagtcgatg cggctacgca
     7621 ggtcacgaac acggcttcgg tgtcgagcag tacgtccgac ccgaggcccg acaacaatac
     7681 gtcatcgttc cccgtcctca cgaagatcag catcaatgac gtgattgtga cggaaggcaa
     7741 cagcggaaca acgcctgcgg tcttcaccgt gagtctgcag cccgcgaatg cgacgctcac
     7801 cacaaccgtc gattaccaga cgacgaatgg cacagccacc gcgggcagcg atttcgtcgc
     7861 cacgtcagga acgctgatat tcctcgccgg cgagactgcg aagaccatca ccgtgcccgt
     7921 cattggtgac tcgatcgtcg aggggaccga gttcttcaac gtgcaactct cgaatgcggt
     7981 caatgcagcg ttccaggata cggccggcac cgggaccatc atcgacgacg atctgggagg
     8041 accgccggca ccgccgtccg tgcagatcgg caacctctcc ttccccgagg gcaatagcgg
     8101 gacgtccaac gtcacgttta cggcaacgtt gtcagccgcg tcaacgaacg tgagccgcgt
     8161 gcgctggcag acgcaggatg gaacggcgac cgcgggatcg gactacgtcg catcgagcgg
     8221 cgagctcgtt tttcagccgg gcgagctcac aaaaaccttc accgtagcca tcatcggcga
     8281 tacgaatttc gagcctgacg agaccttcag catcctcatc gtcagtaccg acaacacctc
     8341 gacggcgccg ggaccggcgg ccgtggctac catcgtcaac gacgacgtcc aggttcctcc
     8401 ccgccatcgc gctgtcggtc attgacgtca ttccggcttc cctcttgaca gaataatgcg
     8461 ccgaacatat tgttagcact cacgaggctt gagtgctaaa cgctcagctt cggacaagag
     8521 agtcatttcg cggcgttctg ccgcgaatcc atccagtatc aaagcgaaag gagagtcaca
     8581 gatgtctatg aaacttcgtc cgttgtatga ccggatcgtc gtcacccgca aggaaacagg
     8641 agagcaggtg cgcggcggca tcatcattcc ggacacggcc aaagagaagc cccagcaggc
     8701 ggaagtcatc gcggttggcg acggcaagtt ttccgaggac gggaagcgca tgaagctcga
     8761 cgtcaagaag ggcgacaccg tcctaatcgg caagtactcc ggcaccgata tcaagatcga
     8821 tgacgtcgag tacaccatcc tccgcgaaga cgaagtcctc gccgtagtcg agtaagcaac
     8881 cagaaagaag gagaaacaac caatggcaaa gcgcattacg tacagtgaag acgcccgtca
     8941 ggcgattctt cgcggcgtca acaagctcgc cgacgcggtc aaagtgaccc tgggcccccg
     9001 tggccgcaac gtcgtcatcg agaagaagtt cggttcccct acgatcacca aggacggcgt
     9061 caccgtcgcc aaggagatcg agctgaagga tgaaatggaa aacgccggag cccagatggt
     9121 ccgcgaggtc gcttcgaaga cctccgacat ggctggcgac ggcacgacca ccgcaacggt
     9181 tctggcccag gccatctacc gcgaaggcat caagaacgtc accgccggcg caaacccgat
     9241 ggacgtcaag cgcggcatcg agatcgccgt ccgcgccgcc gtcgattcga tcgacaagct
     9301 ggccaagccg gttgagggca aggacatcgc ccacatcgga acgatctccg cgaacagcga
     9361 tgacgagatc ggcagcatca tcgcccaggc catggagaag gtcggcaaag acggcgtcat
     9421 caccgtcgaa gaagcgcgcg gcctcgacac cgtcctcgaa gtcgtcgagg ggatgcagtt
     9481 cgaccgcggc tacctctcgc cgtacttcgt caccgacccg gagcgcatgg agtcggtcct
     9541 ggagaacgcc aagatcctca tctacgagaa gaaggtttcc tcgatgaagg acctgctccc
     9601 gatcctcgag cagaccgctc gtcagggcaa gccgctcctc atcatcgctg aagacgtcga
     9661 aggcgaggct ctggccacgc tcgtcgtcaa caagctccgt ggcacgctga acgtcgccgc
     9721 cgtcaaggcg ccgggcttcg gcgaccgccg caaggccatg ctcgaagaca tcgcaatcct
     9781 caccggcggc aaactgatct ccgaggacat cggtgtgaag ctcgagaacg tcaagtggga
     9841 agacctcggc gacgccaagc gcgtcacgat cgataaggac aacaccacgc tcgtcaccga
     9901 caccgacgac aagtcccgca aggaccagat cgccggccgc gtgaagcaga ttcgtggcct
     9961 tatcgacgat acgacctcgg attacgatcg cgagaagctc caggagcgcc tggccaaact
    10021 cgtgggcggt gttgccgtca tcaaagtcgg cgccgcaacc gagaccgaga tgaaagaaaa
    10081 gaaagcccgc gtcgaagacg caatgcatgc gaccaaggcc gctgtagagg aaggcatcgt
    10141 tccaggtggg ggcgtcgcac tccttcgagc gatcgctgcc gtcgaagcgg tcaaggccga
    10201 gggcgatgtg aaggttggcg tcaacgtcat ccgccgcgcg ctcgaagagc cgttccgcca
    10261 gatcgtcttc aacgcaggaa aagaagcggc agtcctgctg aacgagatca agaccaaggg
    10321 cggcaactgg ggctacgacg cccgcaatga tgaagtcgtc gacatgatca gcgcagggat
    10381 catcgacccg gccaaggtga cgaagcaggc gctccagaac gcctcctcga tcgccggcct
    10441 gatgctcacc accgaagctc tcatctccga gatcaaggaa gaagagaagg gtggcggcgg
    10501 cggtaacccc ggtgccggaa tgggcggcgg tatgggcggg atgtactaag tttcaatcaa
    10561 cgcaatccac gaacgggcgg accgcaaggt ccgcccgttt tgttgttgga ccggtttggt
    10621 cgtcatcatc ctggttgcac tcgctccgct tccgccttcg ccaccaccca aggacgtgtg
    10681 gcgtggcgct gagcgatgga gatgtgtcaa cgccgtcatc gttcaagtga aatcaccttg
    10741 ttttaaggta a
//
LOCUS       DATLVW010000791         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_77458, whole genome shotgun sequence.
ACCESSION   DATLVW010000791 DATLVW010000000
VERSION     DATLVW010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77458"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..880)
                     /locus_tag="VNN25_23205"
     CDS             complement(431..880)
                     /locus_tag="VNN25_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94505.1"
                     /translation="MKKHIIHAVAALFLVIVAAPLFAVQRNVVDEVIRMTRAGVSEDA
                     MLNYVDKTDIRIVVTGDDVIAMTEANVPKAVIKEVVDNSGRGSNYRDRAYVSPRVVVA
                     PSYYDPYYYYDPFWYRPSLYLGFGFGGFYGGGFRGGHFGGHGGGHHH"
     gene            1064..>2053
                     /locus_tag="VNN25_23210"
     CDS             1064..>2053
                     /locus_tag="VNN25_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94506.1"
                     /translation="MLKRLFAVTIQLAATASALAQWSQFGANQRHTGNSSVVGQPLVR
                     TLADVVHDPLVPDEIDPGSGDLFVHYAVPLIDGDDVFMSYKELIFIGPFQGIPRWSVH
                     RLHWENGQLVEKWVQPSDWYPIPDGAGIWEPAMQPVIANDSIYVPAAGGTLLQLNRTT
                     GEIIHRINPFGSIDTMTFVSGPPTADSSGNIIYGAFRLQQDAPWFGDILGSWLVRVTP
                     AGTPTIVPFAPLVTNAPAPDAACTWEFSFGGPLHPPSPDAVAPTRTCGGQRPGINVAP
                     AVASDGTIYIVSRAHFNDRWGFLVAVNPDLTPKWSQSLRERLHDGCGVLLPPAN"
BASE COUNT          415 a          650 c          623 g          365 t
ORIGIN      
        1 cccgaacatg acgcgagggt gaagctgggg gacacggcaa gtcgccactt ctcacacccc
       61 tatcgcaacc cttccaatcg agcgccttct ccccccgcgc tttttgcggg gggagaaggt
      121 ggcgaagccg gatgaggggg aagcaaatgc gcagaacgat caaccgcgcc acgtcccaac
      181 tacctgatga cacggcacat gcacggattc agcacttgtg ggaacgcccc ctcatccggc
      241 cttcggccac cttctccccc acaagaagcg tgggggagaa ggcgctcgat tggaaaggtt
      301 gcgagaagtt gagagacgtg cgccagaact gtgaggagac gtgcgcagat gagtagccct
      361 catcacccca aaaaaaaccc gggcgcgagg cccgggcttt cggtcgaccg tgagagaggc
      421 ttactgcgag ttagtgatga tgaccaccgc cgtgaccacc gaagtggcct ccgcggaatc
      481 cgccgccgta gaacccgccg aagccgaagc cgaggtagag gctcgggcgg taccagaacg
      541 gatcgtagta atagtaagga tcgtagtagg acggcgcgac gacgacacgc ggcgacacgt
      601 acgcgcggtc gcggtagttg ctgccgcgtc cggagttatc gaccacttcc ttgatgaccg
      661 ccttcggcac gttcgcttcc gtcatggcga tgacgtcatc gccggtaacg acgatccgaa
      721 tgtccgtctt gtcgacatag ttgagcatgg cgtcctcact gacaccggcc cgagtcatac
      781 ggatgacttc gtcgacgacg ttccgctgca cggcgaagag cggcgcggcc acgatcacga
      841 gaaagagcgc ggcgactgcg tgaatgatgt gtttcttcat gggtttctcc cttagatagg
      901 ttacaaagct aacgggtagt tatgcgacgc ctgagccagc cggcaggctc attcctgtga
      961 gagaagcaaa gtgtaacaag ggaaaccgac ctcctcgatc gaggacggag cgtccgcgaa
     1021 aatgggcaag catgcggcaa cggaaatcgc ataatctctc acgatgctca agcgtttatt
     1081 tgcggtgacg atccagcttg cggcgacggc ctccgcgctc gctcaatggt cccagttcgg
     1141 cgccaaccag cggcacaccg gaaacagctc ggtcgtcgga cagccgctcg ttcgcacgct
     1201 cgcggacgtg gtgcacgacc ccctcgtgcc cgatgagatc gatccaggat cgggcgacct
     1261 cttcgttcac tatgccgtgc cgctcatcga cggcgacgac gtcttcatgt cgtacaaaga
     1321 attgatcttt atcggaccgt ttcaggggat cccgaggtgg agcgtgcatc gtctgcattg
     1381 ggagaacggt cagctcgtcg agaagtgggt gcagccaagc gactggtatc cgattccgga
     1441 cggggcgggg atctgggagc cggccatgca gccggtcatc gccaacgact ccatctacgt
     1501 tccggccgcg ggcgggacgc tcctgcaact caaccggact accggcgaga tcatccaccg
     1561 catcaatccc ttcggatcga tcgacaccat gacgttcgtc tccggtccac cgaccgcgga
     1621 ttcctcaggg aacattatct atggcgcatt ccggctgcag caggacgcac cgtggttcgg
     1681 tgacatcctc ggctcctggc tcgttagggt gacgccggct ggcaccccca ccatcgtgcc
     1741 gttcgcgccg ctcgtgacca acgctccggc accggacgcc gcttgcacct gggagttttc
     1801 tttcggcggc ccgctgcatc ccccatcccc ggacgcagtc gcgccaacga gaacctgcgg
     1861 ggggcagcgg ccgggtatca acgtcgcgcc agcggtcgca tccgacggga cgatctatat
     1921 cgtcagccgc gcgcacttca acgatcgatg gggcttcctc gtggcggtca atcccgacct
     1981 cacgccgaaa tggagccagt cgttacgcga acgccttcac gacggctgcg gcgtactgct
     2041 cccgcctgca aac
//
LOCUS       DATLVW010000792         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_159190, whole genome shotgun sequence.
ACCESSION   DATLVW010000792 DATLVW010000000
VERSION     DATLVW010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159190"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VNN25_23215"
     CDS             <1..1067
                     /locus_tag="VNN25_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HXH94507.1"
                     /translation="GDLAKQVLSYIPRSRVKDTIYFNPAETPIGINFLSYSSEMERNV
                     IADDLFIIFHRLTEGGGPRMDAILKRAIELLLAIPGNTFFDLYRIFTDDMFLHSLVRQ
                     LPDDSAKKFWKETWPKYPHPVTEEPIVTRMSQFDTSKALRQITSSNSGLNFYDVLQSG
                     KIFIAALPKGEIGSDTSSMLGALLVSQMQLAAFRRVKLPRKERRPFYLFIDEFQNFKT
                     SAFNDIVIEARKFQLCLTLATVKTRDLDEQTRSAMEAVETYIFFRLMDDDARKFGGSL
                     GGFNAESLMNLHPHHAIIRRGRAADTATFKVSPPPRPKESFADEIILQTREMYPPSVR
                     LGLASLQGDDDDVKPGLPPE"
     gene            1193..1726
                     /locus_tag="VNN25_23220"
     CDS             1193..1726
                     /locus_tag="VNN25_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HXH94508.1"
                     /translation="MPQKYKTIYADPPWPEYGGGRIKRGADRHYPLMTIPNIKSLNVQ
                     QLADVDCHLYMWVTNNFLQHGLDIMTTWGFTYKTMITWAKDRIGLGQYFRGQTEHVLF
                     GVKGRPPYKTVDGKRQQGATLIAAPRGRHSVKPEAMRRMIETVSYGPYLELFARRAAP
                     GWDVWGNEVENDIQLVS"
     gene            complement(1711..1899)
                     /locus_tag="VNN25_23225"
     CDS             complement(1711..1899)
                     /locus_tag="VNN25_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94509.1"
                     /translation="MTTKHACGSLYLRDVPAATAPYCSAFPYMSRLGLLAEFAPNGKM
                     PPKDDSDKVVAIPGHETS"
     gene            complement(2184..2462)
                     /locus_tag="VNN25_23230"
     CDS             complement(2184..2462)
                     /locus_tag="VNN25_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94510.1"
                     /translation="MSPEGFSPQEQPPDVEALRHEVEENIFQASDIVEQKLPDVPDKQ
                     MLRHLLAAAGHLVGPAYQRDAWRRRAPLLRADLEGALAELRQLEDNES"
BASE COUNT          638 a          862 c          776 g          603 t
ORIGIN      
        1 ccggcgacct tgcgaagcaa gtcctcagct acatcccccg gagccgggtc aaagacacga
       61 tctacttcaa ccccgccgag acgccgatcg gtatcaactt cctctcctac tcctccgaga
      121 tggagcggaa cgtcatcgcg gacgatctct tcatcatctt ccaccggttg accgaaggcg
      181 gcgggccgag gatggacgcg attctgaagc gggcgataga gctcctgctc gcgattccag
      241 gcaacacctt cttcgacctt taccgcatct tcaccgacga catgtttctc cattcgttgg
      301 ttcggcaatt gccagatgat tccgcgaaga agttctggaa ggagacctgg ccgaagtatc
      361 cccaccccgt gaccgaggag cctatcgtca cgcggatgtc gcagttcgac acgtcaaagg
      421 cgcttcggca gataacctct tccaattccg gactgaactt ctacgacgtt ctccaatccg
      481 gcaagatctt catcgctgca ttgccgaaag gggagatagg ttccgacacg tcctccatgc
      541 tcggagcctt gctcgtttcg cagatgcagc tcgcggcgtt ccgcagagtg aagcttccga
      601 ggaaagagcg ccggccgttc taccttttca tcgacgagtt ccagaacttc aaaacctccg
      661 ccttcaacga catcgttatc gaggcgcgga agttccaact ctgcctcacc ctcgcaaccg
      721 tgaaaacccg cgacctagac gaacagacca ggagcgcgat ggaagccgtc gaaacctaca
      781 tcttcttccg gctcatggac gatgacgcgc ggaagttcgg aggttcgctc ggaggattca
      841 acgctgaatc cctcatgaac cttcatccgc atcacgcaat catccgaaga ggcagagctg
      901 cagataccgc aaccttcaag gtttctccgc ctccaagacc gaaggaatcc ttcgccgacg
      961 aaatcatcct gcaaacacga gagatgtatc ctccgtcggt gcgccttggt cttgcttctc
     1021 tccagggcga tgatgatgac gtgaaacccg gactcccacc ggagtagtta tcaaggccta
     1081 ccgagccggc atcagatcgc agttgtaacg cctcgcgcgg tacctttcag cgaaaccgcc
     1141 ataagcgccc ctttactacg ctccttttgc ccaaccgctc cacgcttcgc gcgtgccgca
     1201 gaagtacaaa acaatctacg cagacccgcc gtggcccgaa tacggcgggg gaagaattaa
     1261 gcgcggagcc gatcggcact atcccctcat gaccatccca aacatcaagt cgctgaacgt
     1321 ccagcaactc gccgacgttg attgccacct ttacatgtgg gtgacgaaca atttcctaca
     1381 gcacggcctc gacatcatga ccacgtgggg cttcacctac aaaacgatga ttacgtgggc
     1441 gaaggatcga atcggtctcg gacagtattt ccgcggtcaa accgaacacg ttctcttcgg
     1501 cgtcaaggga aggccgccgt acaagacagt cgatggaaag cgccagcagg gcgcgacgct
     1561 cattgctgcg ccgcgcgggc gacactccgt taaaccagaa gctatgcgaa ggatgatcga
     1621 gaccgtgagt tacggtcctt atctcgaact gtttgcacgc cgcgcggcac ccggctggga
     1681 cgtctggggc aacgaagtcg aaaacgatat tcagcttgtc tcatgaccgg gaatcgctac
     1741 gaccttatcg ctgtcgtctt tcggcggcat cttgccgttc ggggcgaatt cggcgagcag
     1801 accgaggcga ctcatatagg ggaaagccga gcaatagggg gctgtcgcgg cgggtacgtc
     1861 ccgcaggtac aacgagccgc acgcatgctt cgtcgtcatg gtttactaag tcgttgtgtt
     1921 gcaagcgaat ggtaggagtc ggcgtcaata tcgcgcagat tcatcttatt caatccgtac
     1981 ggcgggttac atgcctcctc attgctttga agttccgttc tttcatcttc cttttcgtgg
     2041 cggtgctacg ccacaggccg cactcatgcg agcgcgcgcc agaaatgcgc agcaataaaa
     2101 ggctggccta cctgtagagg cgaaagtagg aggttcccac gctataacta agcttcgtta
     2161 tcttttctaa aggaagacat ccttcaagat tcgttatctt cgagctgacg caattccgct
     2221 agtgctcctt ccaggtccgc tcgaagcagc ggcgcgcgcc tccgccatgc atcgcgctgg
     2281 taggcaggac caacaagatg gccggcagcg gcgagcaggt gcctcagcat ctgcttgtcg
     2341 ggaacgtcgg gcagtttctg ttcgacgatg tcgctcgcct ggaagatatt ttcttcgacc
     2401 tcgtggcgta acgcttcgac atcgggcggt tgttcttgag gtgagaaacc ctcgggactc
     2461 atgagaaata ttcgttggaa gttgaggaga cctttgccaa tcagtgtaag tgccgtgccg
     2521 aggctgtcaa cccgtggcta agggaagggt cttgtaacag gcgaaaaaat gaatcgctcg
     2581 atgcgcgagg gctgctgcat ttctaccgat ccgccttact tcgcgtttac gttgctcgcg
     2641 tggaaaccgg aatcctcttc gaggacaaca gaccgcatct cgcgacgatg gacccgcagg
     2701 gcgtggcgct cgccctaacc gacaatcaat acaacacgag caaggtcagg gcatgagcgc
     2761 gcgggaggtt ctaaccggtc ctcgtcgcga ggcgcttgcg tttctcgcgg agtatcacta
     2821 cctcacgacg ccggactttt accgtctcct tggcaaaggc ggaccacatg agcaacgcg
//
LOCUS       DATLVW010000793         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_282291, whole genome shotgun sequence.
ACCESSION   DATLVW010000793 DATLVW010000000
VERSION     DATLVW010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282291"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1245)
                     /locus_tag="VNN25_23235"
     CDS             complement(142..1245)
                     /locus_tag="VNN25_23235"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH94511.1"
                     /translation="MSEDSLSIQGTLAETTVPDLFRSLLRSGETGIVSLEAIGRNDVI
                     YFKEGKIIFASSSDPDMALGEVLLRGGELTLHQYNQAMERLVVARRIGTLLCELGYLQ
                     PEELLRALERQASAIVLNAIPYRTGSYTIEFTSDLPTEIISLQLNTERLILDGVQSIE
                     YWSLITRGLGRLDRILQQVPDADMRTYSLELTDEESHVLSLLAEPGTVEEVCAHSYLS
                     NFVTCRTLWALLSVNLVSETQGEMVTEQRAAAETEYELEGVVERYNRTYEAIFSLVFQ
                     KIGDHIYDFMDRVVVHLSSETTPYLSGMNLVNEARIDFDQLLNNVIASGSRDYPTVIH
                     NILNQLLYGWICEIKAEFGAEMESEVLKMAEGL"
     gene            1373..2059
                     /locus_tag="VNN25_23240"
     CDS             1373..2059
                     /locus_tag="VNN25_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94512.1"
                     /translation="MRSIVSLLIATFLAAQLTAASLPTQPNDSEWGLLSAEYLRIDGL
                     RKAQPLPPANASRKQMLELVLDNQKKLEPVYVPFLDRVKEYFDRTHDPRAGQVLAREK
                     IIMGDEYMQYLSRYDKALELYKSAVDLDPSSADAKQRVALAEQRRFVSMTAFANVKNG
                     MKEDTVRGLVGLPREDWIKQVVQNGRAYSVWIYPKEDGGASAVYFDNGVVYHTNWNAA
                     APPASQAQTK"
     gene            2074..2757
                     /locus_tag="VNN25_23245"
     CDS             2074..2757
                     /locus_tag="VNN25_23245"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXH94513.1"
                     /translation="MVSDEVLMDDSSHYEVSLTAGQAFIAFVLLLLSLAATFAFGLMI
                     GRGQADERLVVRKEPTVVNEGSVIAAKKNSGKLVDLGIGNDDFKAPPPAVPTMTETAP
                     PAAAITEAQVVPLTESAPSPSTTTAVVAETPKPVAPIAKAPVVKPAVAAPAGPVYAQL
                     LVTSDQKTADALAAKLIDHGFMSSFVERSISEKGTTFKVRVKFSSDAEARSNEAKLKE
                     LSKDVWFVK"
     gene            2742..3458
                     /locus_tag="VNN25_23250"
     CDS             2742..3458
                     /locus_tag="VNN25_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HXH94514.1"
                     /translation="MVREVTTAKGIERALRADRNPLLASHHLRFFKCGPGEYGEGDAF
                     LGVAVPPLRALSKRYKSVEVSEAVALLQSKWHEVRLLALMILVHLYERGDDAGREAIY
                     RAYLANTNRISNWDLVDVSAHKIVGAHLRDRDRTPLLALAKSEMLWERRIAIIATLDF
                     IRQDDFRDTLRIVEALLTDRHDLIHKAAGWMLREVGKRDQAVLERFLRKHARVMPRTM
                     LRYAIERFPAELRAKYMKRD"
BASE COUNT          707 a         1108 c         1083 g          674 t
ORIGIN      
        1 cagcggccaa cgcctgccgt cgttgcactc atcgggtttc aaaccccttc ccgagccgac
       61 gctgcgaggt gggatcaagg cgatgacgct ggcggtgttg gaattgctac ggtaattgcg
      121 cggcccgtca gggccgcgca attaaagtcc ctccgccatc ttcaacacct cgctctccat
      181 ctccgctccg aattcggctt tgatctcgca gatccagccg tagaggagtt gattgaggat
      241 gttgtggatg acggtggggt aatcgcggga gccggaggcg attacattgt tgagcaattg
      301 atcgaaatcg attcgcgctt cattcacgag attcatcccg gacagataag gcgtcgtttc
      361 ggacgacagg tggacgacga cgcggtccat gaagtcgtaa atgtgatcgc cgatcttctg
      421 gaacacgagg ctgaaaatcg cctcgtatgt ccggttgtag cgctcgacca ctccttccag
      481 ctcgtactcg gtctccgcgg ccgcgcgttg ctccgtgacc atctccccct gcgtttcgga
      541 gacgagattc acgctcagaa gcgcccagag cgtgcggcag gtcacgaaat tcgagaggta
      601 gctgtgcgcg cagacttctt ccaccgttcc gggctcggcc agcaggctca gaacgtgact
      661 ctcttcatcc gtcagctcta gcgagtacgt gcgcatgtcg gcatcgggga cttgttggag
      721 gatgcggtcg agacgcccga gaccgcgggt gatcagcgac cagtactcga tgctctgcac
      781 gccgtcgagg atgagccgtt ccgtattgag ttgcagggag ataatctcag tcgggaggtc
      841 gctggtgaac tcgatcgtgt agctgccggt gcggtagggg atggcattga ggacgattgc
      901 gctggcctgc cgctccaacg cccgcagcag ctcctcgggt tgcaggtagc cgagctcgca
      961 aagaagcgtg ccgatgcggc gggccacgac caggcgctcc atggcctggt tgtattgatg
     1021 aagcgtcagc tcgccgccgc gcagtaagac ttcgccgagg gccatgtcgg gatcggagct
     1081 ggaggcgaaa atgatcttcc cttccttgaa gtagatgacg tcattgcggc cgatcgcttc
     1141 gagcgaaacg atgcccgtct cgccgctgcg aaggagcgag cggaagagat cgggaaccgt
     1201 cgtctcggcg agcgttccct ggatactgag actgtcttcg gacatggcga aatagtagtt
     1261 cacgcgaaga cgcgaagaca cgaagagacg aagcgtttga gggttgcgat gcttgctacg
     1321 tggtcttcgc gccttcgcgt cttcgcgtga tcatgtaaaa taaccactcg aaatgcgttc
     1381 catcgtctcc ctactcatcg caacctttct cgctgcgcag ctcaccgccg cgagcctgcc
     1441 cacgcagccg aacgattccg agtgggggct gctcagcgcc gagtacctgc gcatcgacgg
     1501 ccttcgcaaa gcacagccgt tgccgccggc caacgcatcg cgcaagcaga tgctggagct
     1561 cgtgctcgac aatcagaaga agctcgagcc ggtctacgtc ccgtttctcg accgcgtgaa
     1621 ggagtacttc gaccgcaccc acgatccccg cgccgggcag gtgctcgcgc gcgagaagat
     1681 cattatgggc gacgagtaca tgcagtacct ctcgcggtac gacaaagcgc tcgagctcta
     1741 caaatccgcg gtcgacctcg atccatccag cgccgacgcc aaacagcggg tcgcgctcgc
     1801 cgagcagcgg cggttcgtct cgatgaccgc gttcgcgaat gtcaagaacg gcatgaagga
     1861 agacaccgtg cgcggcctcg taggtctgcc gcgcgaagac tggatcaagc aggtcgtgca
     1921 gaacggacgc gcctattccg tctggatcta tccgaaggaa gacggcggcg cgtctgccgt
     1981 ctactttgac aacggcgtcg tttatcacac aaactggaac gctgcggcgc ctcctgcgtc
     2041 gcaggctcag accaagtaac gggagggtgt gcaatggtgt ctgacgaagt cctcatggac
     2101 gattcatccc actacgaagt gtcacttacg gcaggacagg cgttcatcgc cttcgtgctg
     2161 ctgcttctct cgctcgcggc cacgttcgcc ttcggactga tgatcggccg cggccaggcc
     2221 gacgagcggc tcgtcgtccg caaagagccg accgtcgtca acgagggaag cgtcatcgcc
     2281 gccaagaaaa actccggcaa gctcgtcgac ctcggcatcg gcaatgatga cttcaaagcg
     2341 cccccgccgg cagttccgac gatgaccgag actgcgccgc cagccgccgc gatcacggaa
     2401 gcgcaagtgg tgccgctcac cgaaagcgcg ccgtctccgt cgacgaccac cgcggttgtc
     2461 gccgagacgc caaagccggt tgctcccatc gcgaaggctc ctgtcgtgaa accggcagtc
     2521 gccgcgcccg ccggtcccgt ctacgcgcaa cttctcgtca ccagcgatca aaagacggcc
     2581 gacgcgctgg ccgcgaaact gatcgatcac ggtttcatgt cttcctttgt cgagcgctcg
     2641 atcagcgaga agggcaccac gttcaaagtc cgcgtgaagt tctcctcgga cgccgaagcc
     2701 cgatcgaatg aggcgaagct caaagagctc tcgaaagacg tgtggttcgt gaagtaacga
     2761 ccgcgaaggg gatcgagcga gcgcttcgtg cggaccgcaa tccgctcctg gcctcgcacc
     2821 acctccgctt cttcaaatgc ggtcccggcg aatacggcga aggcgacgca ttcctcggcg
     2881 tcgccgttcc gccgctgcgc gcgttgtcga agagatacaa gtcggtcgag gtgtccgagg
     2941 cggtggcgct gctgcagtcg aaatggcatg aagtgcgcct gctcgcgctg atgattctcg
     3001 tccacctgta cgagcgcggc gacgatgccg ggcgggaagc gatctatcgt gcctatctag
     3061 cgaacacgaa ccgtatcagc aactgggacc tcgtcgacgt ctctgcgcac aagatcgtag
     3121 gcgcccattt gcgcgatcgc gaccgcacgc cgctgttggc actggcgaag tcggagatgc
     3181 tctgggaacg ccgcatcgcc atcatcgcca cgctcgactt catccgccag gacgattttc
     3241 gcgacacgct gcgcatcgtc gaggccctcc tcaccgatcg ccacgacctg atccacaaag
     3301 ccgcgggatg gatgcttcgc gaggtaggca agcgcgatca ggccgttctg gaacgctttc
     3361 tccgcaagca cgcgcgagtc atgccgcgca cgatgctgcg ttacgcgatc gaaaggttcc
     3421 cggcggagtt gcgggcgaaa tacatgaaga gagactagca aaccctttgc accacagaga
     3481 cacagaggtc gcagagtaga attcagctct gagtcctcag tgtctctgtg gtgcatttct
     3541 tgtccaacgc ggtccacgcg gtccgccatc cc
//
LOCUS       DATLVW010000794         5336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_403730, whole genome shotgun sequence.
ACCESSION   DATLVW010000794 DATLVW010000000
VERSION     DATLVW010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403730"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VNN25_23255"
     CDS             complement(<1..366)
                     /locus_tag="VNN25_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94515.1"
                     /translation="MSRSRLLPLTGILVAVAAWQIGVSLHPSSLIPVPIAVARGIGEL
                     AARGLLVKYVVASLFRVTWGYLTALLVAVPLGLALGMRRRAEAAINPVLQVLRPISPL
                     AWIPIAILWFGVGDLSAIFL"
     gene            complement(628..2241)
                     /locus_tag="VNN25_23260"
     CDS             complement(628..2241)
                     /locus_tag="VNN25_23260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94516.1"
                     /translation="MKRTLFVIIAIVTAGACLAASPEQSKQFISPVYTIDKIYHSMEG
                     PSSVERVYLGDPAAPAELLWVTGIRTEMVDADGATPQLPELMCHVNVDLDPARHQALF
                     GFRRATAARLMTLSQGMLTAKVPAGFGFPLASNEPLLLFTQVLNLNIQKPQNLKVRHR
                     VTIDYIRARDLTSPIKPLFNVGASGMVQLDDNPLAMAHSMASMPVPAMASVTTDAGHD
                     STSMSSAASCLIGARAPQATSTSADYVDPQGRKMTGHWIVPPGKQVNHSDISWFMNLQ
                     FDTKLHYAAVHLHPFARSLEMRDMTSGKTVFLAKAENPPNAIGLLHVDEFTSAEGVPL
                     LHAHKYSLISTYDNPTTTNADSMASVFLGLDDPELVVPNSDQLAHASATLFDANTLVL
                     HTNIGTITAAFDREHAADTVIQVSRFVLAGAFDATRLIGQKNASIAVTPPMVRTSSLL
                     QPTRLESGVERKPGTVSYCRPGGARMEATILIDMDQPKDPDSTCIGFARIVKGLDLLA
                     AIQPGTSASIVNAETVLRPDAAPKVVAAK"
     gene            complement(2238..2678)
                     /locus_tag="VNN25_23265"
     CDS             complement(2238..2678)
                     /locus_tag="VNN25_23265"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXH94517.1"
                     /translation="MRRQITYWLPPIVWAAMILSASSDVFSASHTGGWIEIVISWFGR
                     SLSPAALDLLNEILRKTGHVAAYGILSALSFRALRGERPGWNPRWAIGAIALAALVAS
                     IDEYHQSFIPSRTGTWHDVVLDTAAALLVQTTIRAAQVLIFRST"
     gene            complement(2781..3200)
                     /locus_tag="VNN25_23270"
     CDS             complement(2781..3200)
                     /locus_tag="VNN25_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94518.1"
                     /translation="MSKQRVKKKVKQKVDVEAILKTLATIREEARRQLPKGIPLPPSA
                     PPEDFAFMDAFVPGDEEAMEAFEMETLAVALETVANELSASIQAAREKALEQAMEIYY
                     TAEKLSRDPEHADLIPVVEQMREAYRKDFGKEIPKRD"
     gene            complement(3263..3820)
                     /locus_tag="VNN25_23275"
     CDS             complement(3263..3820)
                     /locus_tag="VNN25_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94519.1"
                     /translation="MNQNEVSQTNTATAAQDPAVTPESVVEQLRALRSQIPYYGQLPV
                     PTARSLRSVSSLNSDFGQAAINTVGASPTVQLTVGQTAEELQAATEAAARWSMVRDEL
                     KATFDGVASAVLTMKHTLGQSLLLTYTVSKKLVKAPQHADLLPHVALMRKANRLGRGR
                     KAQPPVPAPTPKAEAATEPSSTPNV"
     gene            4714..>5336
                     /locus_tag="VNN25_23280"
     CDS             4714..>5336
                     /locus_tag="VNN25_23280"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH94520.1"
                     /translation="MSESKSRVRFTVYQPGLSPQQVTLGVDSITLGRASDCTIPIKDR
                     FLSRRHAEVVHERGSWYVRDCGSVNGTMLNGVKLAETALLHPGDRIALGDSEVVFQSD
                     VTTSQFIAVDTDSHATNLAISVDEDDKERSSERTQILSTLALEFLADRPMSELFDFIL
                     DRVVFLLRPTRAAIALLGDDRVSFGNVKLRRLDESESNDLSISKTLLR"
BASE COUNT          998 a         1573 c         1721 g         1044 t
ORIGIN      
        1 gagaaagatc gcggagagat cgccgacgcc gaaccagagt attgcgatcg ggatccaggc
       61 cagcggcgag atcggacgca gcacctgcaa caccggattg atcgccgcct cggcgcgacg
      121 ccgcattccg agggccagtc caagagggac ggccacgagg agcgcggtca ggtaacccca
      181 cgtcacacga aaaagtgagg ccacgacata cttgacgaga agcccgcgtg ccgccagttc
      241 gccgatgccg cgcgccacag caatcggcac cggaatgaga gaggaaggat gcaacgaaac
      301 gccgatctgc cacgcggcga ccgcgacaag gatcccggtg agcggaagca gacgcgatcg
      361 cgacacgttg cgccggagga tagcaggttg gattgaacgc tgggagttgc gtaatgggtc
      421 aatttggacg gcttatcgtg gtcgccgacg caaaggcaac cacagagaca cagcgcggca
      481 tagccgcaac caaatgaaac ccaccaaatc tgcaaccaat cagttttggt gaaaatcttt
      541 gcgtcgcgcg cagaaaccgc tggttagtag tacagagaac acagaggttc tctgtgacct
      601 ttgtgcctct gtggtgaacg gtttttccta cttcgccgcc acgaccttcg gcgccgcatc
      661 cggccgcagc accgtctccg cgttgacgat cgacgcgctt gtccccggtt ggatggcggc
      721 gaggaggtcg aggcctttga cgatgcgggc gaagccgatg caggtgctgt cggggtcttt
      781 gggctgatcc atgtcgatca agatcgtggc ttccatgcgt gcgccgccgg ggcggcagta
      841 ggagaccgtt ccgggtttcc gttccacgcc tgattcgagg cgcgtcggct ggagcagcga
      901 ggatgtgcgg accatcgggg gcgtgacggc gatggatgcg ttcttctggc cgatgaggcg
      961 cgtggcgtcg aatgcgccgg cgaggacgaa gcgggacacc tggatcaccg tgtcggctgc
     1021 gtgctcgcgg tcgaaggcgg cggtgatcgt gccgatgttc gtgtgcagga cgagcgtgtt
     1081 ggcgtcaaac agggtggccg aggcgtgcgc tagttggtca gaattgggaa cgacaagctc
     1141 cggatcgtcg aggccgagga agactgaggc catcgaatcg gcattcgtcg ttgtgggatt
     1201 gtcgtaggtg ctgatcagcg agtacttgtg agcatgaagc agcggcaccc cctccgcgct
     1261 cgtgaattcg tcgacgtgca gcaagccgat ggcgttgggc gggttctcag ccttcgccag
     1321 aaagaccgtc ttgccgctgg tcatgtcgcg catttccagc gagcgggcga aggggtggag
     1381 atgaacggcc gcgtagtgca gtttcgtatc gaattgcagg ttcatgaacc aactgatgtc
     1441 gctgtggttc acctgttttc cgggcgggac gatccagtgt ccggtcatct tccggccctg
     1501 cgggtcgacg taatcggccg aggtgctcgt tgcctgcggt gcgcgtgcgc cgatgaggca
     1561 acttgccgca gagctcatcg acgtgctgtc gtgaccggca tcggtggtaa cgctcgccat
     1621 cgccggcacc ggcatcgacg ccatcgaatg cgccatcgcc agcggattgt cgtcgagctg
     1681 caccatcccc gaggcgccga cgttgaagag aggcttgatg ggtgacgtca ggtcgcgggc
     1741 gcggatgtaa tcgatcgtta cgcggtgccg caccttcagg ttctgcggct tctggatgtt
     1801 gagattgaga acctgcgtga agagcagcag cggctcgttg gaggccagcg ggaagccgaa
     1861 gccggccggc actttcgcgg tgagcattcc ctgcgagagc gtcatcagac gcgcggccgt
     1921 ggcgcggcgg aatccgaaga gagcctgatg cctggcagga tcgaggtcga cgttgacgtg
     1981 gcacatcagc tcgggaagtt gcggcgtggc accgtcggca tcgaccatct cggtgcggat
     2041 gccggtaacc cacagcagct ccgcgggcgc ggcagggtcg ccgaggtaga cgcgctcgac
     2101 gctcgaaggt ccttccatcg agtgatagat cttgtcgatg gtgtagacgg gagagatgaa
     2161 ttgcttcgat tgttccggtg acgcggccag acaggcccct gccgtcacga tcgcgatgat
     2221 gacgaagagg gtgcgtttca tgttgaccga aagattagca cctgggctgc gcgaatggtt
     2281 gtctgcacca gcaaggcggc cgcggtatcg aggacgacgt cgtgccacgt gccggttcgt
     2341 gacgggatga acgactggtg atactcgtcg atggaagcga cgagcgcggc cagtgcgatg
     2401 gcgccgatgg cccagcgcgg attccagccg ggccgctcgc cgcgcaatgc gcgaaaggag
     2461 agcgcgctga gaatcccgta cgcggcgacg tggccggtct tgcgcaggat ctcattcagc
     2521 agatcgagcg ccgccggtga gagtgaacgg ccgaaccatg agatgacgat ctcgatccaa
     2581 ccgcctgtgt gcgacgccga gaagacgtcg ctcgacgccg acagaatcat ggcggcccag
     2641 acgatgggag gaagccagta ggtgatttgg cggcgcattg gatggatgca cagtgaatca
     2701 cgcgaagacg ctcacgcgaa gccgcgaagt cgcgaagaac tatttgccct tcgcgacttc
     2761 gcgtcttcgc gtgagccgat tcaatccctc ttcggaatct ctttcccgaa atccttgcgg
     2821 taggcttcgc gcatctgctc gacgaccgga atgaggtcgg cgtgctccgg atctctggaa
     2881 agtttctccg cggtgtagta gatctccatc gcctgttcca gcgccttctc ccgggctgcc
     2941 tggatcgacg ccgacagttc gttggcgacg gtctccagtg ctacggccag cgtctccatc
     3001 tcgaacgcct ccatggcctc ttcgtcgccg gggacgaatg cgtccatgaa ggcgaagtct
     3061 tcgggaggcg cgctgggggg cagagggatg cctttcggta gctgccggcg ggcctcttca
     3121 cggatcgtcg cgagcgtctt gaggatggct tccacgtcga ccttctgttt gactttcttt
     3181 ttgactctct gtttgctcat cgaatcctcc aaaatgccgg acgcgccacg atggcgcgcc
     3241 cggctgaccg ggggttatta ctttagacgt ttggcgtcga cgaaggttcc gttgccgcct
     3301 ccgctttcgg agtgggcgca ggcacaggtg gctgtgcttt gcggccacgc ccgaggcggt
     3361 ttgccttccg catgagtgcc acgtgcggca gcagatcagc atgctgcggc gccttgacca
     3421 gcttcttgct gacggtgtac gtcagcagaa gcgactggcc aagggtgtgc ttcatggtga
     3481 ggactgctga ggcaacgccg tcgaacgttg ctttcagctc gtcgcggacc atcgaccagc
     3541 gcgcagccgc ctcggtggcg gcttgcagct cttcggcggt ctgaccgacg gtcaattgca
     3601 ccgtcggcga cgcgccgacg gtattaatcg ccgcctgccc gaagtccgaa ttgagactcg
     3661 acaccgaccg cagcgaccgc gctgtcggta ccggcaactg accgtagtag gggatctggc
     3721 tccgcagcgc gcggagttgc tccaccaccg actccggcgt caccgccgga tcctgtgccg
     3781 ccgtagcggt gtttgtttga ctgacttcat tttggttcac ggttaactcc tttctgcccc
     3841 gaagggcaat cggttatggg acggcgatcg ggctgtgtgc gcaactcggg cgccgttccg
     3901 ggtggaatgt caaaagggcg gcgtcgcaaa ccgtccgccc gcctgccccc aaaggtagcg
     3961 atcctcgggg gaacggatgt tgtgacgagg ctgggggagc taacgcgtca accccacttc
     4021 tcggggattc cggcttgcca tggctcaagc tcgacggaat cccacctcat tcaaggccgg
     4081 tcctctactt cccaccggtc tctggctcgg gaatcggacg ctatgactca ggcgtcgcct
     4141 ctgcgtggcc ggaaagatag gtccgcggtt tccgaaagtc aatggtgcaa gctcataaaa
     4201 tgcgtaaatc actgagtatg aatgcgaaat tcttctcatt cgcactgaat ctgtctccgg
     4261 tgcgagcgtt gcggacctct cgcaaaggaa ggcgaggtct ctcgcaagag actcggagtc
     4321 tctcgtaaga gactcagagt ctttcgtaag agactcagag tctttcgcaa gggactccgg
     4381 ggtctttcgc aagagactcg gagtctttcg caagagattc ggagtctttc gcaagagact
     4441 cggagtcttt cgcaagagac tccggggtct ttcgcaagag actcggggtc tttcgcaaga
     4501 gactcggagt ctttcgcaag agactcggag tccttcgcaa gagactcgga gtctttcgca
     4561 aaagactcgg gagtctttcg caagagactc ggagtccttc gcaaaagact cagagtcttt
     4621 cacacgagac tcacgtgtct cccactcccg acccccgact ccccactccc gaaacgccaa
     4681 ccctataatg ccctccaacc cggagggcct ttcttgagcg agtcgaaatc ccgtgttcgg
     4741 ttcaccgtct accagcccgg cctttccccg cagcaggtga cgctgggggt tgacagcatc
     4801 acgctcggac gcgcttccga ctgcacgata ccgatcaagg atcgtttcct ttcgcgcagg
     4861 cacgccgagg tcgttcacga gcgcggctcg tggtacgtgc gtgattgcgg gagcgtgaac
     4921 ggcaccatgc tcaacggcgt gaagcttgcg gaaaccgcat tgcttcatcc cggcgaccgc
     4981 atcgccctcg gcgacagcga ggtggtcttc caatccgatg tcaccacttc gcaattcatc
     5041 gccgtcgata ccgattcgca cgccaccaat ctggccatct ccgtcgatga ggacgacaaa
     5101 gagcgcagca gcgagcggac gcagatcctg tcgacgctgg cgctggagtt cctggccgac
     5161 cggccgatgt ccgagctctt cgacttcatt ctcgaccggg tcgtgtttct cctccgcccg
     5221 acgcgcgctg ccatcgcgct cctcggcgat gaccgcgtct cgttcgggaa cgtgaagctg
     5281 cgccggctcg acgagtccga atcgaacgac ctcagcatca gcaagacatt gctgcg
//
LOCUS       DATLVW010000795         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_444195, whole genome shotgun sequence.
ACCESSION   DATLVW010000795 DATLVW010000000
VERSION     DATLVW010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444195"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VNN25_23285"
     CDS             <1..396
                     /locus_tag="VNN25_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94521.1"
                     /translation="LQPNKTDYWQLLQSAHLDVGLSVSYWEEADAPWRARARAALQAA
                     ADASRKSIEKDPELPKLRGYFEQIATSLAVAAGLMEKDSLRDDAARAYVEACAALDHA
                     AALTVGTTPNSKDTDEIRALRQRCAANRH"
     gene            complement(577..2142)
                     /locus_tag="VNN25_23290"
     CDS             complement(577..2142)
                     /locus_tag="VNN25_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH94522.1"
                     /translation="MITITVMPDRVPKSVALAFLAFIVLLAFDCVGHAADIEMADMLI
                     THGTVITMDPARRVIDDGAVAVVGSRIAAVGTTAEILARFRPRQTIDASRKVVMPGLI
                     DGHGHAGHELLKSLAADDDNWDSIVERLYAHGSTPEFWKADAGLAGLERLKFGVTTSV
                     NLFGGGPDVYRVDDPKYADAYLNAVREIGLRWFLGVGPRRGPYPSSFTEWSGAAHKDT
                     QVSFDRQIEVSETVIRAWNGAADGRIKMAVVFPTISPEEKLAGPALDEVKREAGAARD
                     LSRRQHVLFLQDGHTRGTVKYANDVLDLLGSDVILSHATNLTSEEIRLIASTGTRISH
                     NPSSIFSMNGRCPVTELIDAGAVVMLGSDGVAPDRSFDMFRHMFQATRYHRFYFHDAK
                     VLPAGKVLEMVTIDAAKALGMEKEIGSLEPGKKADIILLDWFKPHLVPMNMPVYRVVY
                     YANGEDVSTVLVDGRILMRDRRVLTVDEEKILVAAQRESNLAIQRAGLPNNLTQLPDG
                     FWGTTRLRDAAPR"
     gene            complement(2241..>2521)
                     /locus_tag="VNN25_23295"
     CDS             complement(2241..>2521)
                     /locus_tag="VNN25_23295"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HXH94523.1"
                     /translation="DTHFKILRTAKPGDRLTLESLDGSKSVFVVTDVRIADGRRTRIL
                     LDADVARLTLATCYPFDAVNPGGPLRWVVTAERVSSPVPAAMAVNQHT"
BASE COUNT          523 a          797 c          733 g          468 t
ORIGIN      
        1 ctacaaccga acaagacaga ctactggcag ttgttgcagt cggcgcatct cgacgtcggg
       61 ctctcagtca gctattggga ggaggccgac gccccctggc gagcgcgagc gcgcgccgcg
      121 ctccaagcgg cggccgacgc atcgcgcaag agcatcgaga aggacccgga attgcccaag
      181 ctccgagggt atttcgagca gatagccacc tcgctggccg tggccgcggg gctcatggag
      241 aaggactcat tgcgcgacga cgccgcgcgc gcatatgtcg aagcctgcgc cgcgttggat
      301 cacgccgcgg cgttgacggt cggaacaacg cccaattcca aggacacgga cgagattcgg
      361 gctctgcggc aacggtgcgc agccaaccgg cattgagtgc tcacatcggc cgtttggcat
      421 gcctgcattc gagcgacggt cttcacacct caaaacttgt agctgaggcg cagtccgacg
      481 gtccggggct cagtggcgac actatgaaca agaattgtcc gagcttcgag ccgtaatcac
      541 gtcggccgga tatggagttt aacctccgag cgctaatcac cgcggcgccg cgtcccgcag
      601 acgcgtcgtt ccccagaagc catccggcaa ctgggtcaaa ttgttcggca agcctgcgcg
      661 ttggatcgcc aaattcgatt cccgttgcgc cgccaccagg attttttcct catccacggt
      721 gagcacacgg cgatctcgca tgaggatccg tccatcgaca agtacggtgg aaacatcctc
      781 gccgttggcg tagtagacca cgcgatagac cggcatattc atcggaacga ggtgcggctt
      841 gaaccagtcg agcaagatga tgtccgcttt cttgcccggc tcaagcgagc cgatctcctt
      901 ttccatcccc agcgccttcg ccgcatcgat cgtgaccatc tccagaacct ttccggcggg
      961 cagcaccttt gcatcgtgga agtagaaacg gtgataccgc gttgcctgaa acatgtggcg
     1021 gaacatgtcg aagctgcgat ccggcgccac gccgtccgac cccagcatga cgaccgcgcc
     1081 cgcgtcaatc agctccgtaa ccgggcagcg cccgttcatc gagaaaatcg aactggggtt
     1141 atgcgaaatc cgcgtccccg ttgaggcgat cagccggatt tcctcactcg ttagattggt
     1201 cgcgtgcgac aggatgacgt cggaaccgag caggtcgagc acgtcattcg catacttaac
     1261 ggtgccgcga gtatgtccat cctgaagaaa cagcacatgc tgacgcctcg acaagtcacg
     1321 cgcggcacct gcctcgcgct tcacctcgtc aagcgccggt ccagcgagct tctcctccgg
     1381 gctaatggtg ggaaacacga ccgccatctt gatgcgaccg tcggccgcac cgttccacgc
     1441 ccggatgact gtctctgaaa cctcgatctg ccgatcgaag gacacctgag tgtccttgtg
     1501 cgccgcgccg ctccattccg tgaacgaact gggataggga cctcggcggg gacccacgcc
     1561 tagaaaccag cgaagcccga tttccctgac cgcatttaga taggcatcag cgtattttgg
     1621 atcatccact cggtatacgt ccggcccgcc gccaaacaag ttcaccgacg tggtaacgcc
     1681 gaacttcagc cgttccaggc ctgccagccc ggcgtccgcc ttccaaaatt ccggggtcga
     1741 gccgtgtgca taaaggcgtt ccacgatcga gtcccaattg tcgtcgtcag ccgcgagcga
     1801 cttgagcagt tcgtggcccg catgtccatg tccgtcgatc aggccaggca tcaccacttt
     1861 ccgactggca tcgatggtct ggcgcggacg gaagcgcgcc aatatttccg cagtcgttcc
     1921 gacagccgcg atcctgctcc cgacgaccgc aaccgcaccg tcgtcgatca cgcggcgagc
     1981 tgggtccatg gtgatgacag tcccatgggt gatgagcata tcggccatct cgatgtcggc
     2041 agcatgacca acgcaatcaa acgcaagcag cacgatgaaa gccaaaaacg caagtgcgac
     2101 gctcttgggc accctatcag gcatgaccgt gattgtgatc atttggggaa atccgctgcg
     2161 ggcattggta gtagcggtcg attataggcc gacgtaccgt gagcaactac ctatccaata
     2221 taggctcggc tcagcctggt ctaggtatgt tgattgacgg ccatggctgc cggaacgggc
     2281 gagctcacgc gctcggccgt gaccacccat cgcagcggtc ctccaggatt gaccgcatcg
     2341 aagggataac aggtcgcgag cgtcagccgc gcgacgtcag cgtccagcag aatgcgggtc
     2401 cggcgaccgt cggcgattcg aacgtcggtg acgacgaaca cagacttgct gccgtccagc
     2461 gattcgaggg tgaggcgatc gcccggttta gccgtgcgca atatcttgaa atgagtatca
     2521 c
//
LOCUS       DATLVW010000796         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_611654, whole genome shotgun sequence.
ACCESSION   DATLVW010000796 DATLVW010000000
VERSION     DATLVW010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611654"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VNN25_23300"
     CDS             <1..771
                     /locus_tag="VNN25_23300"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXH94524.1"
                     /translation="VAPEAFQTRSQRIFVRTLPASLAVHLIAIAAVMAARVWNVEFPL
                     QSPKVSVAYSLTQIPDPPPPPPPPPKPVEIKPRPLRELAPPPPPLAVAQIVAPTIIPE
                     IIPEVLPPQPAPPPPPPPVAAPVAVAKGSPNGVVGGTPGGTGRALGGEGITLGDDGRV
                     YVARNVKVPMKELEHEYPHYPDVAKKAKLEGHSLVRYTVGKNGRISDITFLTKSEYPI
                     FDEETLKRVQSWRYQPMTIDGKPVEVVHEIEVNYEYVR"
     gene            842..2206
                     /locus_tag="VNN25_23305"
     CDS             842..2206
                     /locus_tag="VNN25_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94525.1"
                     /translation="MPSNNAAFPYKDQLEMPVIGLEAEFKVFVDEEERTPEEVWRTPS
                     AFIERPTLKRANKSLQLPTGGALYFDGGVIEVVTPVIEIAPQCTGRAVRSLWEQIGFV
                     RDQLTNWETVHGHHVRLEAFSCHVNISFELARGERSRDRTVQKLAKLLIYLLPIPVIV
                     AGANRRSTGIGVRPRRERIELTMDFTPDPGLMAATVALIVGVVREVIAWPSYRLDVLG
                     GRGVPILAGVEPGKHPSRNGWVMRDFHFPRNPFLSDIDERCWPLTDGRVCSLREIAFE
                     TVMVFRDTIRRVSDPFSFRLLLSVMRGETPSLLDLDDRPAAYEDVGRATRWGTVLPEL
                     ENFAAVMRDDDTSVQVLRRRIDLDEKLAPPWSGEPADRRARQRDHERRQSVPLAPSKR
                     LTRSAYEKVFLKLVSGGSLRIGDERLTPVKVQGWYHSIFRNQSGEERMLSIDQLLAHM
                     GSWE"
BASE COUNT          424 a          733 c          678 g          410 t
ORIGIN      
        1 gtcgcgcccg aggcgttcca aacacgtagt caacgcattt tcgtcaggac attgccggcc
       61 tcgctggccg tccacctgat cgcaattgct gcggtcatgg ccgctcgcgt ctggaacgtt
      121 gaattcccct tgcagtcgcc gaaggtcagc gtcgcgtaca gcctgacgca gattcccgat
      181 ccgcctcctc caccgccgcc tccgccaaag cccgtcgaga tcaaaccgcg gccgctgcgg
      241 gagctggcgc ctccaccacc accgctcgcg gtggcgcaaa tcgtcgctcc gacgatcatc
      301 ccggagatca ttcccgaggt tctgccgccg cagcccgcgc caccaccacc acctcctccc
      361 gttgccgcgc cggttgctgt cgcgaagggc tcacccaacg gcgtagtggg cggcacaccg
      421 ggcggaacag gccgcgcgct tggcggcgaa ggcatcaccc tcggcgatga cggccgcgtc
      481 tacgtcgccc gcaatgtcaa agtgccgatg aaggagctcg aacacgagta tccgcattat
      541 cccgacgtgg cgaaaaaggc caagctcgag gggcactctc tcgtccgcta tacggtgggc
      601 aagaacggcc gcatctccga catcaccttc ctcacaaagt ccgaataccc gatcttcgac
      661 gaagaaacgc tcaagcgtgt gcagagctgg cgttatcagc cgatgacgat cgacggcaaa
      721 ccagtcgagg tcgttcacga gatcgaggtg aattacgagt acgtgcggta gcgtgggtta
      781 gccaaacgag gcgaccgcat cacggcaccc gctttgctcc taagcgaagc gacgctccgg
      841 tatgccctcg aacaacgctg ccttccccta caaagatcaa ctggaaatgc cggtcatcgg
      901 cctggaggcc gaattcaagg tctttgtcga tgaggaggag cgaactcccg aggaggtctg
      961 gagaacgcca tcggcattca tcgaacggcc tacgctgaag cgcgcgaaca agtcgctcca
     1021 gctacccacc ggcggcgccc tctacttcga tggcggcgtg atcgaagtgg tgacaccggt
     1081 catcgagatc gcgccgcagt gcacgggacg cgccgtccga agtctctggg aacagatcgg
     1141 cttcgtgcgc gaccagctca ccaattggga gacggtccac gggcatcacg tgcgtctcga
     1201 agcgttcagc tgtcatgtca acatctcgtt cgagcttgcc cgcggtgagc gcagccgtga
     1261 tcggaccgtg cagaagcttg cgaagctgct catttacttg ctcccgattc cggtcattgt
     1321 ggccggcgcg aaccggcggt cgaccggcat cggcgtccgc ccgcgcagag aacgaattga
     1381 gctgacgatg gactttacgc cggatccggg cctcatggct gcgacggtcg cgctcatcgt
     1441 cggcgtcgtg cgcgaggtga ttgcgtggcc ttcgtaccgg ctcgatgtac tgggcggacg
     1501 aggcgttcca atcctcgccg gcgtcgaacc gggaaaacat ccgtcacgca acggctgggt
     1561 catgcgtgac tttcactttc cgcggaatcc gttcctgtcg gacatcgacg aacgctgctg
     1621 gccgctcacg gacggccgcg tctgctcgct gcgcgaaatc gctttcgaga cggtaatggt
     1681 ctttcgcgac accattcgcc gggtctctga cccgttcagc ttccggctgt tgctctcggt
     1741 gatgcgtggc gaaacgccat cgctactcga tctcgatgac cgccctgcag cgtacgaaga
     1801 tgtgggccgc gctacccgct ggggcaccgt tctgccggag ctcgagaact ttgcggccgt
     1861 aatgcgcgac gacgacacga gcgtacaagt gctgcgacgc cgcatcgatc tcgatgagaa
     1921 gctcgccccg ccgtggtccg gcgagccggc agaccggcgt gcgcgccaac gcgatcacga
     1981 gcggcgccaa tctgttcccc ttgcgccgtc gaagcggctc acccgttccg cctacgagaa
     2041 ggtattcctg aaactcgtca gcggaggaag tcttcgcatc ggcgacgagc gtctcactcc
     2101 ggtgaaagtt caggggtggt atcactcgat cttccgcaac cagagcggcg aagagcggat
     2161 gctatccatc gaccagttgc tcgctcatat gggatcgtgg gagtagtaga aacgtgtcgg
     2221 actggagagt ccgacctacg ttcct
//
LOCUS       DATLVW010000797         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_322433, whole genome shotgun sequence.
ACCESSION   DATLVW010000797 DATLVW010000000
VERSION     DATLVW010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322433"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..420
                     /locus_tag="VNN25_23310"
     CDS             241..420
                     /locus_tag="VNN25_23310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94526.1"
                     /translation="MRRVSKALATASLLVALTTSNVYAVPSGGTSPTSLGTKIVRIIR
                     HVIGLDLGDMSWPHP"
     gene            complement(429..1664)
                     /locus_tag="VNN25_23315"
     CDS             complement(429..1664)
                     /locus_tag="VNN25_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94527.1"
                     /translation="MKHADDLDHLAGCARCRQRFAGKTLPTDSGERLARKREFIAAAA
                     RLERERNDTVDLVARHLRETPTAEWPRLAELPALRNNAALEQMSDEVRKRLHRRPTEA
                     LTIAQLSTAVAALIPDGLYPPLVLAQIRANAWKDRASALRYLARYDEALDAASIGEEH
                     LAPFAAVEHDRAIVRMVKGMVLAQIGRLDEALPIMTECRRVFDEHHDSRRHLQAGISE
                     GCVLYEASRYAEAVDVLAGVLPNAVATGDIESQARIHNNLGYCLTHLDDYVRANVHFS
                     EAIARFTDLGFTAEVPRTERGAGRVLLARGQTAAGLAYLRAARRDFTAAGVIEEAGLC
                     ALSIAASLVERGESVEARALMQEVIDEFTAAQLDERAITAVVRLRDAIDVEGATAETV
                     RTVHAFVESLRDEVRSEAN"
     gene            complement(1661..2251)
                     /locus_tag="VNN25_23320"
     CDS             complement(1661..2251)
                     /locus_tag="VNN25_23320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH94528.1"
                     /translation="MIPPSPADTLFESLPSLVRAETDPPPRIEDNEFVQVYEANFGLL
                     VSIAVRKFQVPFGDAEGLANDVFVSYLKHRNEVRELHGWLIGAICNASRHYWRKNDRS
                     IDQLDDEATSTQIDPVSAHVVEWLTARIAAGEALAVLSARHQRILRLRYYEGYSIPEI
                     AEELGVTKKYAQKLVGKILRRAAEFFKASGGPMEKK"
     gene            2334..2960
                     /gene="trmB"
                     /locus_tag="VNN25_23325"
     CDS             2334..2960
                     /gene="trmB"
                     /locus_tag="VNN25_23325"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXH94529.1"
                     /translation="MNASDFVIDPRETGFVRLDVAAIFGNRNRVVLEIGSGKGRFLLS
                     GATEQPDVNYIGIEKSLHYHRVIRDRVIKRGLTNVRLINHDAFLVLRDMLPEASIDEI
                     HIYFPDPWPRKREQKRRIIRAEALVEMRRVLVPGGIGIFVTDHRDYYEAAAPVIEQFF
                     PSERRVPGADDPPRTNYEAKYREEGRPIFEVRFGRDARSGGRALSVVG"
BASE COUNT          555 a          939 c          940 g          568 t
ORIGIN      
        1 gatgacacaa cacgcgtttt gagcacttgt tgacggcgtc ggcggttcgg gattcgagcg
       61 atcgcggaat cgccctcggg cgagacgccc gagtgcccgc gggccgagac gcccgcactc
      121 caataggcaa aatatttctc accgccaacg gtagcaatcc cgccgtttcc gtcacgctag
      181 gggtgagggt gccctcctga aaaacgtccg gcgcgtcgcc gggcactgac cggagggatt
      241 atgcgtcgtg tatccaaggc tctagctact gcttcgcttc ttgttgcgct cacaacctcg
      301 aatgtctatg ccgttccgtc gggcggcact tccccaacct ccctcggaac gaagatcgtc
      361 aggatcatcc ggcatgtgat cgggctggat ctgggcgaca tgtcgtggcc gcatccgtga
      421 gctgacaatt aattcgcctc gctgcggact tcgtcgcgca gactttcgac aaaggcgtgg
      481 acggtgcgta cggtttcggc cgtcgcacct tccacgtcga tggcgtcgcg gagacggacg
      541 acggcggtaa tcgcgcgctc gtcgagttgt gccgcggtga attcatcgat gacttcctgc
      601 atgagtgctc tcgcttccac gctttctccg cgctcgacca gcgaggcagc aatgctcaag
      661 gcacagagtc ctgcctcctc gatcaccccc gctgcggtga aatcacggcg tgccgcgcgc
      721 agataggcga gaccggcggc ggtctggccg cgggcgagca gaacgcggcc tgcgccgcgc
      781 tccgtacgcg gcacctccgc ggtgaagccg agatcggtga accgcgcgat ggcttccgag
      841 aaatgaacgt tagctcggac gtagtcgtcg agatgagtca agcagtatcc gaggttgttg
      901 tgaatgcgcg cttgtgactc gatgtcgccg gtagcgacag cattggggag aacaccggct
      961 aagacatcga ccgcctcagc gtaccgagag gcctcgtaaa gcacacatcc ctcggagatg
     1021 ccagcctgca ggtgacggcg gctgtcgtga tgttcatcga acacgcgacg gcactcagtc
     1081 atgatcggaa gcgcttcatc aagccggccg atctgcgcaa gcaccatccc cttcaccatc
     1141 cggacgatcg cccgatcatg ttcgacggcc gcgaacggag ccagatgctc ctcgccgatt
     1201 gacgctgcgt ccaacgcctc gtcgtatcga gccaggtatc gaagcgcgct ggcccgatcc
     1261 ttccacgcgt tcgcgcggat ctgcgcgagc acgagcggcg gatagagacc atcgggaatc
     1321 aacgccgcca cagcagtcga gagctgcgcg atcgtcagcg cttccgtcgg acggcgatgc
     1381 agacgcttcc gcacttcatc gctcatctgc tccaacgccg cgttgttgcg aagcgcaggc
     1441 agctcggcca ggcgcggcca ctccgcggtt ggcgtctcgc gcagatggcg ggccacgaga
     1501 tccaccgtat cgttgcgctc gcgctcgaga cgtgcggcgg cggcgatgaa ctcacgcttc
     1561 cgggccagac gctcgccgct atcggttggg agggtcttgc cggcgaaacg ctgccggcat
     1621 cttgcacatc cggcgagatg atcaagatcg tcggcgtgct tcatttcttc tccatggggc
     1681 cgccggaagc tttgaagaat tccgctgcgc ggcgaaggat tttgcccacc agcttctgtg
     1741 cgtacttctt cgtgacgccc aattcctcgg cgatctccgg aatgctgtat ccctcgtaat
     1801 agcgaagacg aaggattcgc tggtggcgcg cggagaggac ggcgagcgct tcgccggcgg
     1861 caatgcgggc ggtaagccac tccaccacgt gcgccgaaac gggatcaatc tgcgtcgacg
     1921 tcgcctcatc gtcgagttga tcgatgcttc gatcgttctt gcgccagtag tgccggctgg
     1981 cattgcagat cgctccgatc agccatccgt gcagctcgcg gacttcgttg cggtgcttca
     2041 gatacgagac gaagacatca ttggccagcc cctcggcgtc accgaacggc acctggaact
     2101 tgcggacggc gatcgacacg agcagaccga agttcgcttc gtagacttgt acgaactcat
     2161 tgtcttcgat ccgcggagga ggatcggtct ccgctcgaac gagcgacggc agagactcga
     2221 agagcgtgtc ggcaggcgaa gggggaatca tcagcaggac gggatgagcg aacgatagca
     2281 cttagcaaag atggacgaaa ggaccggata cgttgcttgc gtatgatgcc gccgtgaacg
     2341 cttccgactt cgtcatcgat ccgcgcgaga ccggcttcgt acgtctcgac gtcgcggcca
     2401 tcttcggcaa ccgcaatcgc gtcgtcctcg agatcggctc gggcaaaggg cgctttctcc
     2461 tttccggcgc caccgagcag ccggacgtca actacatcgg catcgagaaa tcgctgcact
     2521 accaccgcgt catccgcgac cgcgtcatca aacgcggtct caccaacgtc cgcctcatca
     2581 accacgatgc ctttctcgtt ctgcgcgaca tgctccccga ggcatcgatc gatgagattc
     2641 acatctactt tcccgatcca tggcctcgga agcgcgagca gaagcgccgg atcatccgtg
     2701 ccgaggcgct ggtggagatg cgacgggtgc tggtgccggg agggatcgga atcttcgtca
     2761 ccgaccatcg cgactactac gaagccgccg cgccggtgat cgaacagttc tttccaagcg
     2821 agcgccgcgt gcccggcgca gacgacccgc cgcgaacgaa ctacgaagcg aagtatcggg
     2881 aggaaggccg gccgatcttc gaagttcggt ttgggcggga tgcgcggtct ggcggccgcg
     2941 cgttgtctgt tgtcggttga ttgccttcgg cgttgtcggg ggcttggagg gtggggggag
     3001 tc
//
LOCUS       DATLVW010000798         5079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_322475, whole genome shotgun sequence.
ACCESSION   DATLVW010000798 DATLVW010000000
VERSION     DATLVW010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322475"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VNN25_23330"
     CDS             complement(<1..1149)
                     /locus_tag="VNN25_23330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HXH94530.1"
                     /translation="MKRLSILILFLAACAAAPQAPIAQPAAQSAQPVHVIIVSTTDRH
                     GWYDSHHDSRTAPPYGGLPLMGGYLQNLRASHEGRVIVVDSGDLFQGTLESNLFEGEP
                     VIKGYNALGYSAVAVGNHEFDYGPVGPDSVARLPGQDPLGALKKNAAAARFPFLSANL
                     TETATGKTPSWAKRYTIVNAGGAKIGIIGLSTPDTPNVTMSANVTTLRFGDPIAATVS
                     AAKEVRAAGADAVIVIAHMGGRCKNIDTNPADPSSCESDQEAMRFLNALPSGTIDAYF
                     AGHTHSQMRHYINDVAALQPSPFGREFSMLDMTVDVANHKVTGTQMAPLTMVCAQVYE
                     HTETCDPKKAPAGATLVPRVFEGSDVAELSGLRDTLKPYLDQVAAKRNQ"
     gene            1303..1677
                     /locus_tag="VNN25_23335"
     CDS             1303..1677
                     /locus_tag="VNN25_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HXH94531.1"
                     /translation="MLIQNKRTMLYQTLATAVAFIHFAFIVFVVLGGLLVLRWPVLKW
                     VHLPAAIWGAVIEIGGFVCPLTRIENAFLRRAGAAGYDDGFIAHHLFGVIYPQGLTRG
                     MEFAIAAFVIVVNTAVYRKVFH"
     gene            1678..2880
                     /locus_tag="VNN25_23340"
     CDS             1678..2880
                     /locus_tag="VNN25_23340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH94532.1"
                     /translation="MVSLAIPQHPVSTTDKPFSSLGLSTPILDIITKIGFQNPTPIQA
                     AAIPLALEGRDVIGLAQTGSGKTAAFSLPLAERLTHGRGVRGLILSPTREIALQTKAF
                     LDVFGVNHELKTACVIGGVKMQPQTDALRNEPDIIVATPGRLLDHVRQRHVKLDAIEE
                     LVLDEADHMLDLGFLPQIRDLLRLLPRDRHTMMFSATMPDSIEMIAKDFMRDPVRIDI
                     TPEGGAAEGITHRLYLVKEEDKRNCLITLLTEELGTTLVFVRRKIDAEWLYHILERRG
                     HPVSRIHADRSQGRRTEALAGFRKGQHRILVATDIAGRGIDVPGIEHVINFDIPENVD
                     DYVHRAGRTARGAALGIVSTIVSWQDVPMVRQIEEILGEKIPRCTAPGIEPWVETEKA
                     PSRTRRRV"
     gene            2915..3358
                     /locus_tag="VNN25_23345"
     CDS             2915..3358
                     /locus_tag="VNN25_23345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94533.1"
                     /translation="MAEDRSIADFVVEKPGLGIRLLVEARNTPADSREWAARFLRNIF
                     EHDEIPDTEYFLLAFRDHLYLWHRPMREPGNPDFEADTATELRPYLSRLNRPLDTLYK
                     SSFELLIQAWLGELVDGTLPESEWLMKSGLAAAVRDGSIRLNLAA"
     gene            3355..3900
                     /locus_tag="VNN25_23350"
     CDS             3355..3900
                     /locus_tag="VNN25_23350"
                     /inference="COORDINATES: protein motif:HMM:NF027613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH94534.1"
                     /translation="MIVYVETNFLLELAYLQERCDSCQLILDFAVARTITLALPAFSA
                     AEARATSHRRASERREFQASLQRHIREISRSEPFRGLSEQSRDVVTALVAGDQEARAR
                     LESAIQTVEEHGITIPLTGEIIRMAYVHELIYSLSSPDALVLASVLSHAEKSAEQKCF
                     ISQDFKAFEDRAVSDEISRVG"
     gene            complement(3986..>5079)
                     /locus_tag="VNN25_23355"
     CDS             complement(3986..>5079)
                     /locus_tag="VNN25_23355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH94535.1"
                     /translation="VTFQAIGTLLLALVLGQIARTFGWRYVKRWALAFVAMFFAIVAV
                     RVYIATSHPWPWWAVYILAQWVFLALLFAGCRDLIDNPFPLKFLGFVTPAALAAAILI
                     VIAAPEFGVLFMIEAGIVSVVAITCFVLIGRITPRTVGWQMMRISLAMMAILYAAYIP
                     LYAMARSGITVPYLDSSSLADLLASVLLGFSMVLIAADFAHRELSDAVAALRIARDQL
                     AIKANTDPLTAALSRHAFHAMPREMSGTVVMVDVDHLKRINDREGHAAGDEAIRAVAN
                     AIRTRVRADDLLFRWGGDEFLVVMPQLPMDLVTQRFADLDRAITTPRGTTLTVSWGAA
                     AFQHADQLDETISTADRAMYARKAMERAG"
BASE COUNT          975 a         1571 c         1589 g          944 t
ORIGIN      
        1 ctgattgcgc tttgcggcga cctgatcgag atagggcttc agtgtgtcgc ggagcccgga
       61 caactccgcc acgtcgctcc cttcgaacac gcgcggcacg agcgtcgcgc ccgccggggc
      121 tttcttcgga tcgcacgtct cggtgtgctc gtagacctgc gcacaaacca tcgtcagcgg
      181 cgccatctgc gtccccgtga ccttgtggtt ggccacatcg accgtcatat ccagcatcga
      241 aaactcacgg ccaaacggtg acggctggag cgcggcgaca tcgttgatgt agtgccgcat
      301 ctgcgagtgc gtgtggccgg cgaagtaggc gtcgatcgtt ccgctcggga gcgcgttcag
      361 gaatcgcatc gcctcctgat cggattcaca gctcgacgga tcggccggat tcgtgtcgat
      421 gttcttgcag cggcctccca tgtgcgcgat gacgatcacg gcgtcggcgc ctgccgcgcg
      481 tacctctttt gccgcgctca ccgtcgccgc gatggggtcg ccgaagcgca atgtcgtcac
      541 gttcgccgac atcgtgacgt tcggcgtgtc gggcgtggaa agaccgatga ttccgatctt
      601 cgcgccgccg gcgttgacga tcgtgtagcg cttcgcccac gatggcgtct tgccggtcgc
      661 cgtctccgtg aggttggccg acaggaacgg aaacctcgcc gctgccgcgt tcttcttgag
      721 cgcgccgagg ggatcctggc cgggaagacg ggcaacggaa tcggggccga ccgggccgta
      781 gtcgaactcg tgattcccga ctgcgaccgc gctgtagcca agcgcgttgt accctttgat
      841 gaccggctca ccttcgaaga gattcgattc gagcgtgccc tgaaacagat cgccggagtc
      901 gacgacgatg actcgcccct catgactggc gcgcagattc tgaagatatc cgcccatcag
      961 cggcaggccg ccgtagggag gagccgttcg cgaatcgtga tggctgtcgt accaaccgtg
     1021 acgatcggtc gtcgaaacga tgatgacgtg aacgggctgc gccgattggg ctgcgggttg
     1081 cgcgatcggg gcttgcggtg cggccgcgca cgcggcgagg aacagaatga ggatggaaag
     1141 ccgtttcatg gggcgcaagt ataggagcgc acgcgtctcg cttgcggcgt gggttcgacg
     1201 gcgcagtctc cgttgtgcca aactcttcac tccttaactt ggattcgtca ccgccgggcg
     1261 agacgcccgc cggccgcagg cgagacacct gcactccttg cgatgctcat tcaaaacaaa
     1321 cgcacgatgc tctaccagac cctcgcgacc gcagtcgcct tcatccactt cgcgttcatc
     1381 gttttcgtcg tccttggcgg acttctcgtg ctgcgctggc cggtgctgaa gtgggttcac
     1441 ctgcctgcgg cgatctgggg agcggtgatc gagatcggcg gattcgtctg tccgctcacg
     1501 aggatcgaga acgcgtttct gcgcagagca ggcgccgctg ggtatgatga cggcttcatc
     1561 gcccatcatc ttttcggcgt gatctacccc cagggcctca cacgcgggat ggaattcgcc
     1621 atcgccgcat tcgtcatcgt ggtcaacacc gcggtttata ggaaagtgtt tcactgaatg
     1681 gtctctttag cgatcccgca acatccggtc tcaacgaccg acaagccttt ctcctccctc
     1741 ggcctcagca cgccgattct cgacatcatc acgaagatcg gatttcagaa tccgacaccg
     1801 atccaggcgg cggccatccc gctcgcgctg gagggacgtg acgtcatcgg cctcgcacag
     1861 accggctccg gcaagaccgc ggccttctca ctgccgctgg ccgagcgcct cacgcacggc
     1921 cgcggcgtgc gcggcctgat cctctcgccg acgcgcgaga tcgccttgca gacgaaggcg
     1981 ttcctcgacg tcttcggcgt caatcacgag ctcaagaccg cctgcgtgat cggcggcgtg
     2041 aagatgcagc cgcaaacgga cgcgttgcgc aacgagcccg acatcatcgt ggccacgccg
     2101 gggcgtctcc tcgatcacgt ccgtcagcgt catgtgaaac ttgacgcgat cgaagagctg
     2161 gtcctcgacg aagccgatca catgctcgac ctcggctttc tcccgcagat ccgcgacctt
     2221 ctgcgcctcc tgccccgcga ccgccacacg atgatgttct cggccacgat gcccgactcc
     2281 atcgagatga tcgccaagga cttcatgcgc gatcccgtgc gcatcgacat cacacccgag
     2341 ggcggcgcgg cggaaggcat cacgcaccgg ctctacctgg tcaaagagga agacaaacgg
     2401 aactgcctga tcaccctgct caccgaggag ttgggaacaa cgctcgtgtt cgtgcggcgg
     2461 aagatcgacg ccgaatggct gtaccacatc ctcgagcgtc gtggtcatcc ggtgtcgcgc
     2521 atccacgccg atcgttcgca gggacgacgc accgaagcgc tggcaggatt ccgaaaagga
     2581 cagcatcgca tcctcgtggc caccgacatc gccggacgcg gcatcgacgt ccccggcatc
     2641 gagcacgtca tcaatttcga catccccgag aacgtcgacg actacgtcca ccgcgccggc
     2701 cgcacagccc gcggggcagc gctggggatc gtatcgacga tcgtctcgtg gcaggacgtc
     2761 ccgatggtcc gccagatcga agagatcctc ggcgagaaga ttccgcgctg cacagcacca
     2821 ggaatcgagc cgtgggtgga gacggaaaag gcaccgtcga ggacgaggag aagggtgtaa
     2881 tcagtcctcc tgagtcgagt agaataacgg cgctatggca gaagatcgtt cgatcgctga
     2941 tttcgtcgtc gagaagcccg ggctcggtat acggctcctc gtcgaagcgc gaaacactcc
     3001 agcagactct cgcgagtggg cggcgcgttt tctgcgcaat atcttcgagc acgacgagat
     3061 accagacacg gagtattttc tgttggcgtt ccgggaccat ctttatctct ggcaccggcc
     3121 aatgcgcgag cccgggaatc ccgactttga ggctgacacc gcgacggaac ttcggcctta
     3181 tctgtcgaga cttaatcgac cgcttgacac gctctacaag agcagctttg agctattgat
     3241 tcaggcttgg ctgggcgagc tcgtggacgg caccctgccg gaatcagaat ggctgatgaa
     3301 gtccggactc gccgccgccg ttcgcgacgg ctccattcga ttgaatctcg ccgcgtgatc
     3361 gtctacgtcg aaacaaactt ccttctcgag ctcgcatatt tgcaggagag atgcgatagc
     3421 tgccagttga tcctcgattt cgcagtggca aggacaatca ctctggccct acctgctttc
     3481 tctgcagcgg aggcgcgcgc cacttcgcac cgccgggcgt ccgagcgccg ggagtttcag
     3541 gcctcgcttc agaggcatat ccgggagatt tctcgctccg agccatttcg cggattgagc
     3601 gaacaatccc gcgatgtcgt tacagctctt gtcgcaggtg atcaggaagc gagggctcga
     3661 ctcgaatcgg caatacaaac cgtggaagaa catggcatca cgattcctct gactggtgaa
     3721 atcatccgga tggcgtacgt gcatgagctc atctattcac tgtcgtcgcc ggatgctctc
     3781 gttctagctt cggtactgtc acatgctgaa aagagcgccg aacagaagtg tttcatcagc
     3841 caggatttca aggcgtttga agaccgtgcc gtgtccgatg agatctccag agttggatga
     3901 aagttcgtat cgaacttcac ggatgccgtc cgctacatta agaacgcgct ccggccgccg
     3961 aagcggtctt agtaccgaac gacactcacc ccgcccgctc catcgctttc ctagcgtaca
     4021 tcgcgcggtc ggcggtgctg atggtttcgt cgagttgatc ggcgtgctgg aaggcggcgg
     4081 cgccccacga gaccgtgagc gtggtgccgc ggggcgtggt gatcgcgcgg tcgagatcgg
     4141 cgaaacgttg cgtgacgagg tccattggca gttggggcat cacgacgaga aactcatcgc
     4201 cgccccatcg aaagagaagg tcgtcggcac ggacgcgggt gcggatggcg ttggccacgg
     4261 cgcggatcgc ttcgtcgccg gcggcgtgtc cttcgcggtc gttgattctc ttgagatggt
     4321 cgacgtcgac catgaccacg gtgccgctca tctcgcgagg catggcgtgg aaggcgtggc
     4381 gcgacagcgc ggctgtgagc ggatccgtat tcgccttgat ggccagctgg tcgcgggcga
     4441 tccggagcgc ggccacggcg tcgctgagct cacggtgagc gaagtcggct gcgatcagca
     4501 ccatgctgaa gccgagcagc accgaggcga gaagatcggc cagcgatgag gaatcgagat
     4561 agggaacggt aattccgctg cgcgccatcg cgtagagagg gatgtaggcg gcgtagagga
     4621 tcgccatcat cgcgagcgag atccgcatca tctgccagcc gacggtacga ggagtgatgc
     4681 ggccgatgag gacgaagcag gtgatcgcga caacgctgac gatgcctgcc tcgatcatga
     4741 agagcacgcc gaattcggga gcggcgatga cgatcagaat cgccgcggcc agcgccgcag
     4801 gcgtcacgaa tccgaggaac ttgagcggga agggattgtc gatgagatcg cggcagccgg
     4861 cgaacaagag cgcgaggaaa acccactgcg caaggatgta taccgcccac cagggccacg
     4921 gatgcgaggt ggcgatgtag acgcgcacag ccacgatggc aaagaacatc gccacgaatg
     4981 ccagcgccca gcgcttcacg taacgccagc cgaacgtccg cgcgatctgc ccgagaacga
     5041 gggcgagcag cagcgtcccg atcgcctgaa acgtcacgg
//
LOCUS       DATLVW010000799         4188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_116874, whole genome shotgun sequence.
ACCESSION   DATLVW010000799 DATLVW010000000
VERSION     DATLVW010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116874"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1132
                     /locus_tag="VNN25_23360"
     CDS             74..1132
                     /locus_tag="VNN25_23360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH94536.1"
                     /translation="MPSATATDLRVNPFLHIDADRIYDPLTDRSLLAGEPEYEALRRV
                     LDGGAVDESLEQGGWLVRGDISRAHRLKIVSLETMIACNQKCYFCPVSIAPREDFDMP
                     VAFFERIVGELTAYRDTLESVFLQSYNEPTLDRRFVEFCRTLFAAKLPVAVLSNGSGL
                     TPAKVDALIESGPLRYICINLSTLDRDRYIRDRGEDHLGVVLRNLDYVKSRPLAAVMK
                     IVVLGKGDAEHDADFESIRDRFAGSLFTVEQHVVMDRAGWLDIGLKPDAPKKQLAGCD
                     NIGSRPLQHLHITPRGQCVLCCEDYDEKYIVGDLTRNTIAEVLAGPELAKMRRWVYGI
                     EEAPDDFMCRDCVFARTR"
     gene            complement(1183..1905)
                     /locus_tag="VNN25_23365"
     CDS             complement(1183..1905)
                     /locus_tag="VNN25_23365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXH94537.1"
                     /translation="MRFLFVALALSLALGPAEAQRSRAVRSPVRLLWIGAHPDDEALV
                     APILGSACVEDINDCAILVLTSGSEVRSAELQRAGAALHAQVTQWDLPDVFDVSEWGD
                     RAALVSKLASYIVSESPAVIYTFDPRHGSSCHPAHREAGQLTLDAVATLGTHAPPVVL
                     LETLIVRDAESRITGFAEATPSATTIDATSTWHYLVNDAAIHESQFDAEQIALLQTIP
                     ADQRRVWLLPSGATVQNITSCP"
     gene            2243..2950
                     /locus_tag="VNN25_23370"
     CDS             2243..2950
                     /locus_tag="VNN25_23370"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH94538.1"
                     /translation="MICVIVPTYNEPPLASFLDRLAAVIAPIGGEILVVDDSGPSIHD
                     ELRAEAEVVRAVPVRVIAGEHRGKGAAVRRAIAESRGDIVFYIDADTDDERMARIPEF
                     VQLIESEGYDVVIAERGWKGRTPLRAFFSIALHLMQCILIFRSVRFFDTQCGFKAFRR
                     EAALRMASLQTLDRGLYDVEYLAIAVTNRFRVARVPVAQATVSRPTRVPAILHLPLHL
                     FDLLSVKRNLMRGRYSA"
     gene            2947..>4188
                     /locus_tag="VNN25_23375"
     CDS             2947..>4188
                     /locus_tag="VNN25_23375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH94539.1"
                     /translation="MRRRLIVDASIALLLAAVVWTLYHKVTNLWLTLDDAYTIHLVVD
                     HGLRSFFLDSAVWPQKLYTPLLLIVTEGELAAFGLDIARWYLAQLAMIAASSIALYAL
                     LRRYFDQPAAAAASLMFVASVSLCSLTMALSGVHYFVALVFGAIAANVYVTALRGGRI
                     SLAVLSAALYLTAMLAKETVVPLVVLLIVLPERDWRTRARFALPHAVALIGYLALRRA
                     VIGTLVGGYGWAVLPGEWPHLIATLPFHLVLAMGGIALVSLIAIGALLALRSRQAIVL
                     FVVAMVLAVGPIVPVSKVMQPRFALMPWLVLSIAFVAGVSTLKRRAITIALLVLAPLA
                     TIASNRIEWRDEFNLTRRTSDEARFFFFDMPPNGLLRTPAVPPAAMSEIDWLKTVYAH
                     RPAGASWFYDDFFLCSVGTAGK"
BASE COUNT          693 a         1298 c         1353 g          844 t
ORIGIN      
        1 cccccgaaat gacatcccca actccccttg tttagacggc gaacctaccc tcgccgtaca
       61 ctctcgatca gacatgccct cagccaccgc cacggatctc cgcgtcaatc cctttctgca
      121 cattgacgcg gatcggatct atgacccgtt gaccgatcgt tctctcctgg ctggtgaacc
      181 tgagtacgag gcgctgcggc gggtcctcga cggcggtgcc gtggatgaga gcctcgagca
      241 gggtggctgg ctcgtacgcg gcgacatctc ccgcgcgcat cggctgaaga tcgtctcgct
      301 ggagacgatg atcgcgtgca atcagaagtg ctacttctgc cccgtctcga tcgcgccgcg
      361 cgaggacttc gacatgccgg tcgcgttttt cgagcggatc gtcggcgagc tcacggcgta
      421 ccgggacacg ctggagagcg ttttcctgca gagctacaac gagccgacgc tagaccggcg
      481 tttcgtcgaa ttctgccgca cgctcttcgc ggcgaagctg ccggtggcgg tgctgagcaa
      541 cggatctggc ctgacgccgg cgaaggtcga tgcgctcatc gagtcgggcc cgcttcgcta
      601 tatctgcatc aatctttcga cgctcgaccg cgaccgctat atccgcgacc gcggcgaaga
      661 tcacctcggc gtcgtgctcc gcaatctcga ctatgtgaaa tcgcgtccgc tcgccgcagt
      721 gatgaagatc gtcgtgctcg ggaaaggtga tgctgagcac gatgccgatt tcgaatcgat
      781 ccgcgaccgt ttcgccggga gcctgtttac cgtcgagcag cacgtcgtga tggaccgtgc
      841 cgggtggctc gacatcggcc tcaagcctga cgcaccaaag aaacagttgg caggttgcga
      901 caacatcgga tcgcgcccgt tgcagcatct gcacatcacg ccgcgcggcc aatgcgtcct
      961 ctgttgcgaa gactacgacg agaaatacat cgtcggcgat ctcacgcgaa atacgatcgc
     1021 cgaggtgctg gcggggccgg agctggcaaa gatgcggagg tgggtctacg gcatcgaaga
     1081 agcgccggat gacttcatgt gccgcgattg cgtgttcgca agaacgcgat gagagttttt
     1141 gatccgcaga tgacgcagat aaagcgcaga taaagtccgg tatcagggac acgacgtgat
     1201 gttctgcacc gttgcgccgg atggcaacaa ccagacgcgg cgctgatcgg ccggtatcgt
     1261 ttgcagcagg gcaatctgct cggcgtcgaa ctggctctcg tggatggctg cgtcgttgac
     1321 gaggtagtgc cacgtgctag tggcgtcgat cgttgtcgcc gaaggagtgg cctcagcaaa
     1381 gccggtgatg cgtgattccg cgtcgcgcac gatcagtgtt tcgaggagaa ccaccggagg
     1441 ggcgtgggtg ccgagggtgg cgacggcgtc gagtgtgagc tggccggctt cgcgatgcgc
     1501 tggatgacac gacgaaccgt gccgcgggtc gaatgtgtag atgactgccg gcgactcgct
     1561 gacgatgtac gacgcgagct tcgacacaag tgcagcgcgg tctccccact ccgagacgtc
     1621 gaatacgtca ggaaggtccc actgtgtaac ctgtgcgtgc agcgcggccc ctgctcgctg
     1681 caactcggcg gaacgcacct ccgagccgct cgtgaggacg aggatcgcgc aatcgttgat
     1741 gtcttccacg caggcggagc cgaggatcgg cgccaccagc gcttcatcat cgggatgggc
     1801 tccgatccaa agcagccgga cgggtgagcg aaccgcacgc gaccgttgtg cttcagccgg
     1861 cccgagggcc agcgaaagtg caagcgcgac aaacaggaat cgcatcgttg ctgatacgcg
     1921 gcgatcatgg attccgtcgt cgggagtcgg gagtcggggg tcgggggtcg ggggtcggag
     1981 ccgtcggatg atcaactggc tccgaccccc gacacccgat cagcgtctcc cggaccgagg
     2041 agtggctggc gtcgggagtc gtgggtcggg cgtcggagcc gtcgcatgat caaccggctc
     2101 cgacccccga ctcccgatca gcgacacccg ccccgagggg tttcggagag cgtttcaatg
     2161 atcaactggc tccgaccccc aactcccgat cagcgactcc cgacaagcga ctcccgggcc
     2221 gcctgcctga taatgcgccc gcgtgatttg cgtgatcgtc cctacctaca acgagccgcc
     2281 gcttgcatcg tttctcgatc gtttggccgc agtgatcgcg ccgatcggcg gggagatcct
     2341 cgtggtcgac gacagcggtc cttcgattca tgacgagctt cgcgccgagg ctgaggtcgt
     2401 gcgggctgtg ccggtgaggg tcatcgctgg cgagcatcgc ggcaaaggtg ctgccgtccg
     2461 gcgcgcgatc gcggagtcgc gaggggatat cgtcttctac atcgacgccg acacggacga
     2521 cgaacggatg gcccggattc ctgagttcgt tcagttgatc gaaagcgaag gttatgacgt
     2581 cgtcatcgct gagcgggggt ggaaaggacg gacgccgctg cgggcgttct tctcgatcgc
     2641 ccttcatctg atgcagtgca ttctgatctt tcgttccgta cgtttcttcg acacgcagtg
     2701 cgggttcaag gcattccgcc gggaggccgc gttacgcatg gccagcttgc agacgctcga
     2761 ccgcggtctg tacgacgtcg aatacctcgc catcgcggtg acgaatcgat ttcgcgtggc
     2821 acgcgtgccg gttgcgcagg ccaccgtctc acggccgacg cgcgttcccg cgatcctgca
     2881 ccttccgctt catctcttcg acctcttgag cgttaagcga aatctcatgc ggggccgcta
     2941 ctcggcatga gacgccggct gatcgtcgat gcgtcgatcg cgctgcttct cgcagcggtg
     3001 gtctggactc tgtaccacaa ggtcacgaac ctctggctga cgctggacga cgcctacacg
     3061 attcatctcg tcgtcgatca cgggctgcgc tcgtttttcc tggatagcgc ggtctggccg
     3121 cagaagctgt acacgccgct gctgctcatc gtcacggaag gggagctggc ggcattcggg
     3181 ctcgacatcg cacgctggta tctcgcgcaa ctggcgatga tcgcggcgtc ctcgattgcg
     3241 ctctacgcgc tgctgcggcg ctatttcgat caaccggccg cggctgcagc ttcattgatg
     3301 ttcgtggcgt cggtatcgct ctgcagcctg accatggcgc tgtcgggggt tcactacttc
     3361 gtggcgctgg tgttcggcgc gatcgctgcg aacgtgtacg tcaccgccct gcgcggcggc
     3421 cgcatatcgc tcgccgtgct atccgcggct ctttacttaa ccgcgatgct ggcgaaggag
     3481 acggtcgttc cgctggtggt tcttctgatc gtgcttccgg agcgggactg gcgtacgcgc
     3541 gcacgtttcg cccttcccca tgcagttgcg ctcatcggct atctggcact gcgccgcgcc
     3601 gtgatcggca cgctcgtcgg cggttacgga tgggcagtcc tgcccggcga gtggccgcac
     3661 ttgatcgcga cgcttccgtt tcatctggtg ctggcgatgg gcgggatcgc tctggtctcg
     3721 ctgattgcga tcggcgctct cctcgcgctt cgcagccgtc aagcgatcgt gctgttcgtg
     3781 gtggcgatgg tcctggcggt cgggccgatc gttccggttt cgaaggtgat gcagccgcgt
     3841 ttcgcgctga tgccgtggct cgttctgtcg atcgcgttcg tggccggcgt cagcacattg
     3901 aagcggcgcg cgatcacgat tgcgctgctc gtccttgcgc cgctggcgac gatcgcctcc
     3961 aaccgcatcg agtggcgcga tgaattcaat ctcacgcgca ggacttccga cgaggcacga
     4021 ttctttttct tcgacatgcc gccgaacgga ctgctacgca caccggccgt tccacctgcg
     4081 gcgatgagcg agatcgattg gctgaagacg gtctacgcac accggccggc gggcgcgtcg
     4141 tggttctacg acgacttctt cctttgcagc gtcgggacgg ccggaaag
//
LOCUS       DATLVW010000800         6522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_367877, whole genome shotgun sequence.
ACCESSION   DATLVW010000800 DATLVW010000000
VERSION     DATLVW010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367877"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..416)
                     /locus_tag="VNN25_23380"
     CDS             complement(72..416)
                     /locus_tag="VNN25_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXH94540.1"
                     /translation="MHELSIALSIVEGAEEEAARCGAAGVRSVYLRLGLLAGVVKEAL
                     LFAYEQACEGTLLEGSQLVIEDVPVLVQCPVCHEPRSPHSLWRLECATCDTPAAGIAQ
                     GRDLQIFAMEVV"
     gene            complement(536..1045)
                     /locus_tag="VNN25_23385"
     CDS             complement(536..1045)
                     /locus_tag="VNN25_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXH94541.1"
                     /translation="MSVLVAGIGNIFLGDDGFGVEVVRRLASEELPPGVKVKDFGIRG
                     FDLAYALMEDHELSILVDATPQGGTPGTLYTIEVDPGQIDRAADATIDTHAMNPVRVL
                     QLVKAMGGEPRSILVVGCEPAILEDENGMIGLSDPVEAAVDDAVSMVRSLIAGHEQRQ
                     RSIPIDATV"
     gene            complement(1042..1362)
                     /locus_tag="VNN25_23390"
     CDS             complement(1042..1362)
                     /locus_tag="VNN25_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94542.1"
                     /translation="MSDWDPFASSTVDSVSVFGHELRRGDRVRLWPQKTADIVDLALA
                     GKEATIEAIEQDLEDRVLFAVVIDDDPGNDMGMLRQPGHRFFFSPDEVEPLALDADVP
                     RDLA"
     gene            complement(1359..2327)
                     /locus_tag="VNN25_23395"
     CDS             complement(1359..2327)
                     /locus_tag="VNN25_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94543.1"
                     /translation="MSDRIERIVNAVLYEGYMLYPYRASSVKNRQRWNFGVVVPEVYR
                     SAVDPSERSVLKTECLLETQMDTTVSICLRFLQLVKREVTGGATSIDPLEGCDETIER
                     ELALPELRPSGMSPFRVAIVIPDSTSETTENGQTIVRTNEAIRGEVVVESKYAGDDAV
                     LLSVFIANSTPIDASAASRDDALRRSLLSAHAVLTAHDGDFVSLIDPPDRFAEAVASC
                     RQDGLYPVLAGEEGDRQTVLAAPIILYDHPQVAPESPDNLFDGTEIDEILTLRIQTLS
                     DDEKREIRGADARTRHLLERADSLDRESLARLHGTLRRERPDGSAP"
     gene            complement(2324..2995)
                     /locus_tag="VNN25_23400"
     CDS             complement(2324..2995)
                     /locus_tag="VNN25_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HXH94544.1"
                     /translation="MPDLDFAVEGAEAIPYAAAPAIGFRVRIRNADPFEAIRNVALQV
                     QLQIEPAQRSYNAGEKERLNDLFGDPSRWGQTLKTLLWTRAQLFVAPFTGETVAEVLV
                     HCTFDFNVAVTKYFAGLEAGFVPVTLLFSGTVFYEGDGRLQIGQISWSREATHRLPLS
                     VWRTMMDLYYPNTAWMCLERPVFDRLIDYKTRRGIETLDRALDDVLDAAEEMRSGLAA
                     EAVQR"
     gene            complement(2988..3632)
                     /locus_tag="VNN25_23405"
     CDS             complement(2988..3632)
                     /locus_tag="VNN25_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HXH94545.1"
                     /translation="MPSLNRDTRSLGILQRFVKRKSDSEERCELCSAPLRGGHQHLWE
                     IAKRQAVCSCDACAILFSDQSGPRFRRIPRDIRSLAAFALSDEQWASLNIPVDVAFFY
                     RDTNAGRTIAMYPSPAGATESALTFDSWTELENDNTVLATMQPDIEALLVRRGRAAHK
                     HYLVPIDECYRLVGLIRMHWRGLSGGSEVWIEINRFFDDLDQRARPAREEAAHA"
     gene            complement(3635..3811)
                     /locus_tag="VNN25_23410"
     CDS             complement(3635..3811)
                     /locus_tag="VNN25_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94546.1"
                     /translation="MPSSDAPSHSITDRKGEETATTDHSGRDGETRNERDATSINPDK
                     RRPIAPGMPELPPA"
     gene            complement(3837..4661)
                     /locus_tag="VNN25_23415"
     CDS             complement(3837..4661)
                     /locus_tag="VNN25_23415"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXH94547.1"
                     /translation="MPESALQQRTNEIESLVSKLDTLGDDEARATSLALVRALTDFYG
                     SGIERILEITAERTGVETIDHLCEDPLVSSLLLLHGLHPLDMATRVEQALERVRPYLG
                     SHGGGVELAGIDGSIVRLRMKGTCQGCPSSTRTMKLAIEDAVYEAAPEVTGIVAEGQE
                     AEAKLSVRSTEAQGGWEEVGVTTLADGFSTTLVVGGRSVVFCRLGESLYAYGSVCARC
                     GAGLEDSTLESASLVCAACKEHFEIAGAGRGRGEPAVHLEPFPLLIEDGRAMVAVH"
     gene            complement(4664..6475)
                     /locus_tag="VNN25_23420"
     CDS             complement(4664..6475)
                     /locus_tag="VNN25_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXH94548.1"
                     /translation="MAIETLIEPREAKPQGKPNLVEMNWDPITRIVGSLGIFTKIDFE
                     KKRVAECHSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHATCAVYAQNMAFGV
                     KTPSLGEWIINLGEAAEYMFDHNIFQDNLVGVDYCEQMVKETNPAVWDKAQRTPSPNA
                     DKHGYKTIGDIMTSLNLFTGSFYREALQMSRMTREMFCLMEGRHVHPSTLYPGGTGTV
                     ATIQLFTDYIVRLMKYVEFMKKVVPMHDDLFNFFYEALPGYEKVGLRRTLLACWGSFN
                     DPDVVDYTYRNMDQWGKAMYVTPGVVVDGKAVTHDLVKINLGIRILLGSSFYDDWAKE
                     ETFVKYDPLGNAVDQRHPWNQTTIPKPQKRDFNGNYTWVMSPRWYDERTKDYLALETG
                     GGPIARLWSTALANMVNIGYVKATGTSVQINLPKTAMLPEAQFEWKIPAYGVNTIERN
                     RARTYFQAYAAACALYFAERALEDLKAGRTKTWAEFSVPQEAIGCGFHEAVRGVLSHH
                     VVIRDGKIANYHPYPPTPWNANPRDVYGTPGPYEDAVQNTPIFEENGPDKFKGIDIMR
                     TVRSFDPCLPCGVHMYVGNGKVIETKHSPMFGAAAGA"
BASE COUNT         1190 a         2020 c         2047 g         1265 t
ORIGIN      
        1 cgcgcgcgcc aggacgtcat tcttcttcat gacgttgcgg cgtacctcaa ccaggcgcgg
       61 ctcggagctc atcagacgac ttccattgcg aaaatctgaa ggtctcttcc ttgtgcgatg
      121 ccggcggcag gcgtatcgca cgtcgcgcat tcgagccgcc acaaggagtg aggagaacgc
      181 ggctcatgac agacggggca ctgaacgagc actgggacgt cctcaatcac cagttgcgat
      241 ccctcgagca gcgttccttc gcaggcctgt tcgtaagcga acaatagtgc ctctttcacg
      301 acgccggcga ggaggccgag gcggaggtag accgatcgga cgccggcggc accgcaacgc
      361 gccgcttcct cttcggcacc ttccacaata ctcagcgcta tggagagctc gtgcattaca
      421 tcgcgcggat tttcagatag cgcaccaggt ctttggagaa aaacacgagc cccaatagaa
      481 acccgatcac cacaatgccg agcagaatct tttcgaacag atccatgtga cctcctcaaa
      541 ctgtggcatc gatgggaatc gagcgttgcc gttgttcgtg gccggcaatg agcgacctca
      601 ccatcgagac cgcgtcatcg accgccgctt ccaccgggtc gctgagtccg atcatgccgt
      661 tttcatcttc gagaatcgcc ggctcgcaac cgacgaccag gatcgagcgc ggttcgcctc
      721 ccatcgcttt gaccaattgc agaacgcgca ccggattcat cgcgtgcgta tcgatcgtcg
      781 catccgcggc gcgatcgatc tggccgggat cgacttcgat cgtgtagagc gttcccggcg
      841 tgccgccctg tggcgtggcg tcgacgagaa tggaaagctc gtgatcctcc atcaacgcgt
      901 aggccagatc gaagccgcgg atgccgaagt ctttgacctt cacgccgggg ggtagttcct
      961 cggacgccag acgccggacg acttctacgc cgaatccgtc gtcgccgagg aagatgtttc
     1021 cgatgccggc aacaaggacg ctcatgcgag gtccctcggc acatctgcat cgagcgcgag
     1081 cggctcgacc tcgtcgggcg agaagaagaa tcggtgtccg ggctggcgca gcatgcccat
     1141 gtcgttgccg ggatcgtcgt caatgacgac ggcgaagagg acgcgatctt cgaggtcctg
     1201 ctcgatcgct tcgatcgtcg cttccttgcc ggcaagggcg aggtcgacga tgtcggcggt
     1261 cttctgcggc cagaggcgga cgcggtcgcc gcgccgcagt tcgtggccga agacggagac
     1321 gctgtcgacg gtggacgaag cgaacggatc ccaatcgctc atggcgcgct tccgtcaggc
     1381 cgctcgcggc gcaaggtgcc gtgaagccgg gccagtgact cgcgatcgag gctgtcggcg
     1441 cgctccagca ggtggcgtgt tcgcgcgtcg gcgccgcgga tctcgcgctt ctcgtcatcg
     1501 ctcaacgtct gaatgcgaag cgtgaggatc tcgtcgatct ccgtgccgtc gaagagattg
     1561 tccggactct ccggagcgac ttgcgggtga tcgtaaagaa tgatcggcgc agcgagcaca
     1621 gtttgccggt cgccttcttc ccccgccaga accggataca gcccatcctg acggcaactc
     1681 gcgaccgcct cggcgaagcg atccggcgga tcgatcagcg aaacgaagtc gccgtcgtgt
     1741 gcggtcagca cggcgtgggc ggagaggagc gaacggcgaa gcgcatcatc gcggctcgcg
     1801 gcgctcgcat cgatcggcgt ggagttcgcg atgaaaactg aaagcagcac ggcatcgtca
     1861 cccgcgtatt tcgattccac gacgacctcg ccgcggatcg cttcgttcgt tcggacgatc
     1921 gtctgaccgt tctcagtcgt ctcgctggtg ctatccggaa tgacgatggc tacacgaaac
     1981 ggactcattc ctgaagggcg cagctcggga agagcaagct cgcgctcgat cgtctcgtcg
     2041 caaccttcca gcggatcgat cgatgtcgcg ccgccggtca cttcgcgttt caccaactgc
     2101 agaaagcgca ggcagatcga gaccgtcgtg tccatctgcg tttccaacag gcactcggtc
     2161 ttgagcacag atcgctccga cggatcgact gcacttcggt acacctcggg gacgacgact
     2221 ccgaagttcc agcgctgcct gttcttcacc gacgacgcgc gatacggata gagcatgtag
     2281 ccctcgtaca aaacggcatt gacgatccgt tcgatgcggt cgctcatcgc tgcacggcct
     2341 ccgcggcgag gccgctgcgc atctcctcgg cggcgtcgag aacgtcgtcg agcgcgcggt
     2401 cgagcgtttc gattccgcgc cgcgtcttgt aatcgatcaa ccgatcgaac accggccgct
     2461 ccagacacat ccacgccgtg ttcggataat agagatccat catcgtgcgc cagacgctca
     2521 gcggaagccg gtgcgtggcc tcgcggctcc aggagatctg accgatctgc agtcgtccgt
     2581 ccccttcata aaacacagtg ccgctgaaaa ggagcgtgac gggaacgaag ccggcttcca
     2641 gaccggcgaa atacttcgtc acggcgacat tgaaatcgaa cgtgcaatga acgagcacct
     2701 cggccacagt ctcgccagtg aacggcgcca cgaacagttg cgcgcgcgtc cagaggagcg
     2761 tcttcagcgt ctgtccccag cggctcggat cgccgaaaag atcgttgagc cgctccttct
     2821 cgccggcgtt gtaggagcgc tgcgccggct cgatctgcag ttgcacctgc agcgcgacat
     2881 tgcggatcgc ctcgaacgga tcggcgttgc ggatgcggac tctgaatccg atcgccggcg
     2941 cggccgcata cggaatcgcc tcggccccct ccaccgcgaa gtcgagatca ggcatgtgcg
     3001 gcctcttcgc gcgcgggtcg ggcgcgctgg tcgagatcat cgaagaagcg attgatctcg
     3061 atccagactt cgctgccgcc cgacagaccg cgccagtgca tgcgaatcag tccgacgagc
     3121 cggtagcact catcgatcgg cacgaggtag tgcttgtggg ctgcgcgtcc ccgccgcacg
     3181 aggagcgctt cgatgtccgg ctgcatcgtg gccaggaccg tgttgtcgtt ctcgagctcc
     3241 gtccacgaat cgaaggtcag cgcggattcc gtggcgccgg caggactcgg atacatcgcg
     3301 atcgtgcgtc cggcgttggt gtcgcggtag aagaacgcga cgtcgaccgg gatgttgagc
     3361 gaggcccact gctcgtcgct caaggcaaac gcggcgagcg agcgaatgtc gcgtgggata
     3421 cgacggaatc gcggcccgct ctgatcgctg aagagaatcg cgcaggcatc gcacgaacag
     3481 accgcttgcc gtttcgcgat ctcccacaga tgctgatggc cgccgcgcaa cggagcgctg
     3541 cagagctcac aacgctcttc cgagtcgctc ttgcgcttga cgaagcgctg caggatgccg
     3601 agggagcggg tatcgcggtt gagactgggc atgatcaggc cggaggcagc tcgggcatgc
     3661 ccggagcgat cggccgccgt ttgtcgggat tgatgctggt ggcgtcgcgc tcgttgcggg
     3721 tctcgccatc gcgtccggag tgatcggtcg tcgcggtctc ctcacctttg cggtccgtaa
     3781 tcgagtggga cggcgcatcc gagctgggca tcgcttcctc cttcgttgca actcgttcag
     3841 tggacggcaa ccatggcgcg gccgtcttcg attaacagcg gaaacggctc gagatgaacg
     3901 gccggctcac cccgtccgcg tccggcaccg gcgatttcga aatgttcctt gcatgcggca
     3961 cagacgagcg atgccgattc gagcgtggag tcttcgagcc ccgcgccgca tcgcgcacag
     4021 acgcttccgt acgcgtagag actctcgccg aggcggcaga acacgacgga gcgcccgccg
     4081 acgacgagcg tggtcgagaa cccgtcggca agcgtggtga cgccgacctc ctcccacccg
     4141 ccttgggcct ccgtactcct tacgctcagt ttcgcctcgg cctcctgccc ctcggcgaca
     4201 atgcctgtca cctcgggcgc cgcttcgtac acggcgtctt cgatggccag cttcatcgtg
     4261 cgcgttgacg atgggcatcc ctgacacgtc cctttcatgc gcaggcggac gatgctgccg
     4321 tcgatgccgg cgagctccac gccgccgccg tgcgatccga ggtacgggcg gacgcgctcg
     4381 agggcctgct cgacccgcgt tgccatgtcg agcggatgca gcccgtgcag cagcaggagc
     4441 gagctgacga gcgggtcttc gcaaagatga tcgatcgtct cgacgccggt gcgttccgcg
     4501 gtgatctcga gaattcgctc gatgcccgat ccgtaaaaat cggtcagcgc gcggacgaga
     4561 gcgagcgacg tcgcgcgcgc ttcgtcgtcg ccgagggtgt cgagcttcga gaccagcgac
     4621 tcgatctcgt tcgtccgctg ctgcagggcc gattcgggca tgctcacgct ccggccgccg
     4681 caccgaacat cggcgagtgc ttcgtctcga tgaccttgcc gttgccgacg tacatgtgca
     4741 cgccgcacgg cagacaggga tcgaaactgc gcacggtgcg catgatgtcg atgcccttga
     4801 atttgtcggg gccgttctcc tcgaagatcg gcgtgttctg cacggcatct tcgtacgggc
     4861 ccggcgtgcc gtagacgtcg cgcggattgg cgttccaggg cgtgggcggg tacgggtgat
     4921 agttggcgat cttcccgtcg cggatgacga cgtgatgcga gaggacacca cgaactgctt
     4981 catggaaccc gcagccgatt gcctcctgcg gcacgctgaa ttccgcccag gtcttcgtgc
     5041 gcccggcttt gagatcttcg agcgcgcgct cggcgaagta gagcgcacag gcggcggcat
     5101 aggcctggaa ataggtacgt gcgcgattgc gctcgatcgt gttcacccca tacgccggaa
     5161 tcttccattc gaactgcgcc tcggggagca tggccgtctt cggaagattg atctgtaccg
     5221 acgtgccggt cgctttcacg taaccgatgt tgaccatgtt cgcgagcgcg gtcgaccaga
     5281 ggcgcgcgat cggtccgccg ccggtctcca gcgcgagata gtccttcgtc cgctcgtcat
     5341 accagcgcgg cgacatgacc catgtgtaat tgccgttgaa atcgcgcttc tgtggtttcg
     5401 gaatggtggt ctggttccag ggatggcgct gatcgaccgc attgccaaga gggtcgtact
     5461 tgacgaaggt ctcttctttg gcccagtcgt catagaacga tgagccgaga agaatccgga
     5521 tgccgaggtt gatcttgacc agatcgtggg tgactgcctt accgtcgacg acgacgccag
     5581 gcgtcacgta catcgccttg ccccactgat ccatgtttcg ataggtgtaa tcgacgacgt
     5641 cgggatcatt gaacgagccc cagcaggcca gcaacgtgcg gcgcagtccg accttttcgt
     5701 atcccggcaa tgcctcgtag aagaagttga acaggtcgtc gtgcatcggg acgaccttct
     5761 tcatgaactc gacgtatttc atcagccgga cgatgtagtc ggtgaagagc tggatggtgg
     5821 ccacagtgcc ggtaccgccc ggatagagcg tcgacgggtg cacatggcgc ccttccatca
     5881 ggcagaacat ctcacgcgtc atgcggctca tctgcaacgc ttcacgatag aacgaaccgg
     5941 taaagagatt gagcgacgtc atgatgtcgc cgatggtctt gtagccatgc ttgtcggcgt
     6001 tcggcgacgg cgttcgctgc gctttatccc agacagcggg attggtctct ttgaccatct
     6061 gctcgcagta atcgacgccg accagattgt cctggaagat gttgtgatcg aacatgtact
     6121 cggcggcttc gccgaggtta atgatccatt cgccgagcga aggcgtcttt acgccaaagg
     6181 ccatgttctg cgcatagacg gcgcaggtgg cgtggttgtc gccgcaaatg ccgcagatcc
     6241 ggctggtgat gaagtgggcg tcgcgcggat ccttgccctt catgaagata ctgtagccgc
     6301 ggaaaatcga cgacgtgctg tggcattcgg ccacgcgctt tttctcgaaa tcgatcttgg
     6361 tgaagatgcc gaggctgccg acgatccgcg tgatgggatc ccagttcatc tcgacgagat
     6421 tgggtttgcc ctgcggcttt gcctcgcgcg gttcgatgag agtttcgata gccataattc
     6481 accttattca gttgtgacat gttgaggatc ccggcgatgg ga
//
LOCUS       DATLVW010000801         6045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_322574, whole genome shotgun sequence.
ACCESSION   DATLVW010000801 DATLVW010000000
VERSION     DATLVW010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322574"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1278)
                     /locus_tag="VNN25_23425"
     CDS             complement(121..1278)
                     /locus_tag="VNN25_23425"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXH94549.1"
                     /translation="MIETRIARRYLWAARKRAHTAFLSSISMLGLAVGVATLLISLAL
                     MAGLQGQIKRRLIASSPQLLIEPAGAPTIDDAKAIETAARRLGVTDVRPLVTGIGWGA
                     NDRERRGRPMRIRSGAARELPSNDEIMVTRDFAAAIALDTGEMITVVSPRTRLTPFGP
                     MPVWRKYRIARLSSEPVNDESADGILNLGETESLFGTGGRPTSIEMYGPEERAEEIQS
                     ALAGQFPGVQVKTWKEINRPLFLALRLEKIVMFVTISMIIFVAALNLISSLSMLILEK
                     RPSVGVLRTLGATERTILIIFLEVGLLIGISGTILGNLFGLSASWLIDHYHMVPLPAG
                     IYPIAYLPLTIDPSDVIGVNAVAIVLSIIATWYPARMASRLDPIAAIREEA"
     gene            complement(1360..2883)
                     /gene="lysS"
                     /locus_tag="VNN25_23430"
     CDS             complement(1360..2883)
                     /gene="lysS"
                     /locus_tag="VNN25_23430"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXH94550.1"
                     /translation="MADHEETQQTEVPESELIRVRREKLERIVGLGFDAYPTKADVDT
                     TIAEVVAKHGEKTAAELEAAPVQVKIAGRIMAIREFGKTAFLVLSEKTARIQVYCRKD
                     TLPEREWALYQNLDTGDWVEASGTLFRTKTNELSVKAAHLGFLVKALRPLPSKWHGLT
                     DVEQRYRQRYVDLIVNDGVKATFETRAKIVRSIRNYFDTHGFIEVETPMMQPIASGAA
                     ARPFRTHHNALDIDLFMRIAPELYLKRLIVGGLSRVYEINRNFRNEGISTQHNPEFTM
                     LEFYEAYADYNDLIALTEELLSALAAEACNSDTVAFRGNEIRFTKPFARYTMKEAIAH
                     FGSIDPADIEDRGKLETLVVKRAHRDPADVTKMNTGKLIAELFEAVSEEHLIQPTFIT
                     DFPTEVSPLSKQRRDDLEIVERFELYIGGMEVANGFSELNDADEQDRRFRQQLGEREA
                     GDDEAMQMDEDYVNALEYGMPPTAGEGIGIDRLAMILTDSPSIRDVILFPLMRPRET"
     gene            3072..3314
                     /locus_tag="VNN25_23435"
     CDS             3072..3314
                     /locus_tag="VNN25_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXH94551.1"
                     /translation="MNVSVEEVTRDPEGFLHRVIEGETLVVTQHDRPVAEIRPIEGVR
                     RPRPFGLAAGSFVVPDDFDDPLPADLLDAFEGRSDS"
     gene            3311..3562
                     /locus_tag="VNN25_23440"
     CDS             3311..3562
                     /locus_tag="VNN25_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94552.1"
                     /translation="MTTSGTVELQTELDVFERLRLNLLDRAAGKYALVKGSDLVDIFG
                     SEVEAIRDGYRRFGNEAFLVKHIVEADVPLNFTSFNLGM"
     gene            3704..3997
                     /locus_tag="VNN25_23445"
     CDS             3704..3997
                     /locus_tag="VNN25_23445"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HXH94553.1"
                     /translation="MIDTGAHSTVLNPDVVSRLGIKPVGVAAIVTPSTTAALICNRYH
                     INVYFSEDFVVENVFAIEAPMGGVPYQCLIGRDILRGATLTYAGPHNRFTIEF"
     gene            complement(4072..4680)
                     /locus_tag="VNN25_23450"
     CDS             complement(4072..4680)
                     /locus_tag="VNN25_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94554.1"
                     /translation="MSNEPKPSRFSARTGFFLGVATAAVIGALAFAAVRYDGNSKATI
                     TAPVTQVAASAATTETFPLAEHVAAMNGTTQEAAATPTAPALVSPKAPATTLLSGTIE
                     LDPSAASSVTGTVTVFVIARDKKGKGHPIFAKRLDVASFPTKFTLGPQDSMMGGAPPD
                     TVSVEARIDLDHDAMTKEPGAPSAKIESVTMGSKDLTLTLKP"
     gene            complement(4677..4838)
                     /locus_tag="VNN25_23455"
     CDS             complement(4677..4838)
                     /locus_tag="VNN25_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94555.1"
                     /translation="MNGIVTGGWNFVLAAYIVTGTVLVAYSIHTIRELRQKLQIRAAL
                     TRVTKGTSS"
     gene            complement(4835..5536)
                     /gene="ccsA"
                     /locus_tag="VNN25_23460"
     CDS             complement(4835..5536)
                     /gene="ccsA"
                     /locus_tag="VNN25_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXH94556.1"
                     /translation="MKRTTIGLPIIFFFASAVCLFAGILDGLLIAPAEQHMGQVQRIM
                     YIHVPTAWTAMLVLTWAFLCAVMFLFRASWRWDSRLEAALEVSVLLCFLLCVQGSIWA
                     KPTWGVWWDWDPRLTTTAVLLMAFVGIVALRHFLADAAQRATWSAVATIIAYVDVPIV
                     YFSVKWWNSLHQQPSSASTISRDFLIPLMLNFAGMLFLVIGLMTIRGRTALLRLEDEM
                     LPPPLPAAIPTEVMA"
     gene            5707..>6045
                     /locus_tag="VNN25_23465"
     CDS             5707..>6045
                     /locus_tag="VNN25_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94557.1"
                     /translation="MNNLIRRAALMLLTVALLALPLSATDSRTRAAAPAPPTAKPRFS
                     PSQVEAYATASDLAFIRPGLKIIVNSITIGADRKPVIDISMTDSLDQPLDRLGKTTPG
                     PISPSFILAWY"
BASE COUNT         1140 a         1831 c         1893 g         1181 t
ORIGIN      
        1 tgacagaggc cacggtgacg cgctcgccct gatcgtcgaa cgtcagcgtc agatcgtgat
       61 gaacgatgtt cgcgcacgat gccacgaaga aaaatacggc cgccgctgcg aatgttcgtt
      121 tcatgcttcc tcccggatcg ctgcgatcgg atcgagccgc gacgccatcc gcgcggggta
      181 ccacgtggcg atgatcgaca ggacgatggc cacggcgttg acgccgatca catccgacgg
      241 atcgatcgtg agcggcaggt atgcgatcgg atagatgccc gccggcaacg gcaccatatg
      301 gtagtgatcg atgagccacg acgcgctgag accgaagagg ttgccgagga tcgtgccgga
      361 gatgccgatg aggaggccca cttcgagaaa gatgatgagg atcgtccgct cggttgcgcc
      421 gagagtgcgc agaacgccga cggagggacg tttttcgagg atgagcatcg agagtgagga
      481 gatgaggttc agcgcggcca cgaagatgat catcgagatc gtcacgaaca tcacaatctt
      541 ctccagacgc agcgccagaa agagcggccg gttgatctcc ttccacgtct tgacctgcac
      601 gcccggaaac tgcccggcca gcgccgactg aatctcctcg gcgcgctcct ccgggccgta
      661 catttcgatc gatgtcggcc tgccgccggt gccaaagagg ctctccgtct cgccgaggtt
      721 gaggatgccg tcggccgact catcgttgac cggttcggaa gagagacggg cgattcggta
      781 ctttcgccag accggcatcg ggccgaacgg cgtgaggcgt gtgcgcgggg agacgactgt
      841 aatcatctcg ccggtgtcga gcgcgatcgc ggcggcgaag tcgcgcgtga ccatgatctc
      901 gtcattggaa ggcagctcgc gggcagctcc cgatcggatg cgcatcggac gtccgcgccg
      961 ctcgcgatcg ttggctcccc agccgatgcc ggtgacgaga ggccgcacgt cggtgacgcc
     1021 taaccgccgc gcagcggtct cgattgcttt cgcgtcgtcg atcgtcggcg cccccgccgg
     1081 ctcgatcagc agttgcggac tcgacgcaat gagccgccgc ttgatctgcc cctgcaatcc
     1141 cgccatcagc gccagcgaga tgagcagcgt ggccacgccg accgcaagtc caagcatcga
     1201 gatcgacgaa agaaacgccg tatgcgcccg cttgcgagcg gcccagaggt aacggcgggc
     1261 gattcttgtt tcgatcattg ggatggaaga aaaacgtggt ctaacgtagg tcggactctc
     1321 cagtccgaca tcacgggcag tcggactaga gagtccgacc tacgtttctc ttggcctcat
     1381 caacggaaac aagatcacat cccgtatcga cggcgagtcc gtcaggatca tcgccaggcg
     1441 atcgatgccg atgccttctc cggccgttgg cggcatgccg tactcgagag cgttgacgta
     1501 atcctcgtcc atctgcatcg cctcgtcgtc gccggcttcg cgctcgccga gttgctggcg
     1561 gaagcgccgg tcctgttcgt cggcatcgtt cagctccgag aatccgttcg ccacttccat
     1621 ccccccgatg tagagctcga atcgctcgac gatctcgaga tcgtcccgtc gctgttttga
     1681 aagtggtgag acttcggtcg gaaagtccgt gatgaacgtc ggctggatga gatgctcctc
     1741 ggataccgct tcgaacaact ccgcgatcag cttgcccgtg ttcatcttcg tcacgtctgc
     1801 aggatcgcga tgcgcacgct tgaccaccag cgtctcaagc ttgccgcggt cttcgatgtc
     1861 cgccggatcg atcgatccga aatgcgcgat cgcttccttc atcgtgtagc gggcgaacgg
     1921 tttcgtgaag cggatctcgt tgccgcggaa cgcaaccgta tcgctattgc acgcctcggc
     1981 tgccagagcg ctcagcagct cctcggtcag cgcgatcaag tcgttgtagt cggcgtacgc
     2041 ctcgtaaaac tccagcatcg tgaactcggg gttgtgctgc gtggagatcc cttcgttgcg
     2101 gaagttgcgg ttgatctcgt agacgcgcga gagcccgccg acgatgagcc gcttgaggta
     2161 cagctcgggt gcgatgcgca tgaagaggtc gatgtcgagc gcgttgtggt gcgtgcgaaa
     2221 cggccgcgcc gccgcgccgc tggcgatcgg ctgcatcatc ggcgtctcca cttcgatgaa
     2281 gccatgcgtg tcgaagtagt tgcggatgct gcgcacgatc ttggcgcgcg tctcgaacgt
     2341 ggccttgacg ccgtcattga cgatcagatc aacgtagcgc tgccggtagc gctgctcgac
     2401 gtcggtcagc ccatgccact tgctcggcag cggccgcagc gccttcacga gaaagccgag
     2461 gtgcgcggcc ttgacggaaa gctcattggt cttcgtgcgg aaaagcgtgc ccgacgcctc
     2521 cacccagtcg ccggtgtcga ggttctgata aagcgcccac tcccgctccg gcagcgtgtc
     2581 tttgcggcaa tagacctgaa tgcgcgccgt cttctcggaa agaacgagaa aggccgtctt
     2641 cccgaactcg cgaatggcca tgatgcgccc ggcgatcttg acctgaaccg gcgcggcttc
     2701 cagctctgcc gccgttttct cgccgtgctt cgccaccacc tcagcgatgg tggtatcgac
     2761 atcggccttg gtcggatacg catcaaaccc caacccaacg atgcgctcga gcttctcccg
     2821 ccgaacccga atcaactccg attcgggaac ttccgtctgc tgcgtctctt cgtgatctgc
     2881 catgcggcgc acatgatatt cgagggatgg tcgtgaattg ctccgaggca atcgctattg
     2941 ggtcacgcgg agacgcggag atgcggagta gaaccttcgc gtcttcgcgc cttcgcgtga
     3001 gaagagaacc aaacatcatc acgacgagcg tcaaccaatg cggtgttgtt ctttgctaaa
     3061 cttgaaccgc catgaacgtc agcgtggaag aggtgacgag agatccggaa gggtttctcc
     3121 atcgcgtgat cgaaggcgaa acactcgtcg tgacgcagca tgaccggccg gttgcggaga
     3181 ttcgcccgat cgaaggcgta cgccggccga ggccctttgg tctcgcggcg ggctcgtttg
     3241 tcgtgcccga tgattttgac gatcctcttc ccgccgatct gcttgacgca ttcgaaggca
     3301 ggagcgattc atgaccacat ccgggaccgt cgaattgcag actgagctcg acgtgttcga
     3361 gcggctcagg ttaaacctcc tcgatcgggc tgcgggtaag tacgcgctcg tgaagggtag
     3421 tgacctcgtc gatatctttg gcagtgaggt ggaagcgatt cgcgatggct accggcggtt
     3481 tgggaacgag gcattcctcg tgaagcacat cgtcgaggcg gacgtgcccc tgaacttcac
     3541 gtcgttcaac ctggggatgt gattgccaac attcaccagt actgtcgccg accttcttgc
     3601 ctccggtccg ggtcttcaga tttctgttgg gccatcacgt gagctgatca acgcgctcac
     3661 accgttgggc gttcgtgtcg cgccgcctca actcattgcg gcggtgattg ataccggcgc
     3721 gcatagcacg gtgttgaatc cagatgtcgt atcgcgcctc ggcatcaagc cagtcggtgt
     3781 tgccgcgatt gtcacgccat cgacgaccgc ggcgctgatc tgtaatcgct accacatcaa
     3841 cgtctacttc tccgaagact tcgtcgtcga gaacgtcttc gcgattgagg cgccaatggg
     3901 cggcgtgccg tatcagtgcc tcatcggccg cgacatcttg cgcggcgcga cgttgaccta
     3961 cgcaggaccg cacaaccgat tcacaatcga gttctgaccg ggccgggtca ctggctcacg
     4021 cgaagacgcg aagacgcgaa gtagaacccc gcgtccccgc gtgacaaaac cctacggctt
     4081 caacgtcagc gtcaaatcct tgctgcccat cgttaccgat tcgatcttcg ctgatggcgc
     4141 gccgggctct ttcgtcatgg cgtcgtgatc caggtcgatt cgggcttcta cggagacggt
     4201 gtcgggtggg gcgccgccca tcatggagtc ctgggggccg agggtgaatt tggtggggaa
     4261 ggaggcgacg tcgaggcgct tggcgaagat cgggtggcct ttgcctttct tgtcgcgggc
     4321 gatcacgaat accgtcaccg ttccggtgac gctgctcgcg gcggatggat cgagctcgat
     4381 cgttccggag agcagagtcg ttgcaggggc tttcggcgat accagcgctg gggcggtcgg
     4441 cgtggctgcg gcttcctgcg ttgtcccgtt catcgcggcg acatgctcgg cgagcggaaa
     4501 cgtttcggtc gttgcggcag aggcggcgac ctgcgtgacc ggcgcagtga tcgttgcttt
     4561 cgaattgccg tcgtagcgga ctgcggcgaa ggcgagggcg ccgatgacgg ctgctgttgc
     4621 gacgccgagg aagaagccgg tgcgggcgga gaagcgggat ggtttcggtt cgttgctcat
     4681 gatgacgttc ctttcgtgac tctggtgagt gctgcgcgga tctggagttt ctgccgcagc
     4741 tcgcggatgg tgtgaatcga ataggcgacc aggaccgtcc cggtcacgat gtatgcggcc
     4801 agaacgaagt tccagcctcc ggtaacgatg ccgttcatgc catcacctcc gttggaattg
     4861 cggcgggcag cggcggcggc agcatctcat cttcgagacg gagcagagcc gtgcggccgc
     4921 ggatcgtcat caggccgatg acgagaaaga gcatgccggc gaaattcagc atcagcggga
     4981 tgaggaaatc gcgcgagatc gtcgacgcgc tcgacggctg ctgatgaagc gagttccacc
     5041 acttcaccga gaagtagacg atcgggacat cgacgtacgc gatgatcgtt gctaccgcgg
     5101 accaggttgc gcgttgtgcc gcatcggcga ggaagtgacg cagcgccacg attccgacga
     5161 acgccatcaa tagaactgcc gtcgtcgtta gccgtggatc ccaatcccac cacacgcccc
     5221 atgtcggttt ggcccagatc gagccttgaa cgcagagaag gaagcagagg aggacggaga
     5281 cttcgagcgc cgcttcgagc cggctgtccc agcgccagct tgcgcggaag agaaacatca
     5341 ccgcgcagag gaaggcccag gtcagaacga gcatggccgt ccacgctgtc ggaacgtgga
     5401 tgtacatgat tcgctgtacc tggcccatgt gctgctcggc aggggcaatt agcaggccgt
     5461 ccagaattcc agcaaataaa cagacagcgg aagcaaaaaa gaagatgatg ggcaggccaa
     5521 tcgttgttcg tttcatgcta cggcacgata cggacacgcc gctcccgcat ccgcggcccc
     5581 ggtcacgaga cgcggtgaca cgtgtcacct gacagcggtg cggcggcgca tacccgatcg
     5641 ttccaagtga ccctaccgta tgcgcgtgat gtctcacatg aatgtaacgg tcgtttggag
     5701 gaccgaatga acaacctgat caggcgcgcc gcattgatgc ttctaaccgt cgcgttgctg
     5761 gcgcttccgc tatcggcaac cgactcacgg acgcgggccg ccgcgcccgc gcccccaacc
     5821 gcgaaacccc gcttctcccc gagccaggtc gaggcctatg cgactgcgag cgacctcgcc
     5881 ttcattcgcc cgggtctgaa gatcattgtc aactcgatca cgatcggcgc ggatcggaag
     5941 ccggtcattg atatcagcat gactgactcg ctcgaccagc cgctcgaccg cctcggcaag
     6001 acgacgcccg gcccgatctc gccgagcttc atcctggcct ggtac
//
LOCUS       DATLVW010000802         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_367984, whole genome shotgun sequence.
ACCESSION   DATLVW010000802 DATLVW010000000
VERSION     DATLVW010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367984"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VNN25_23470"
     CDS             complement(<1..180)
                     /locus_tag="VNN25_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94558.1"
                     /translation="MIGTVDAHTIGVVVNDGGPIVMCSGSGRRHRSKLFVSSQSASIE
                     FRGDELVVMAADEARF"
     gene            424..1116
                     /locus_tag="VNN25_23475"
     CDS             424..1116
                     /locus_tag="VNN25_23475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH94559.1"
                     /translation="MKRAIITALCVAVLAVPSLIASGSSGGGMGSAPSPSMQQQRSPH
                     DMAVDYYNAAERRLDGLAKTHDEMKASTDAQKVAKLQAKIAKGLENAAADFERAVKND
                     ATLYQAYSELGFTLRKMGKFDESLAAYDKSLSIQPDYTPALEYRAEAYLGLNRIDDAR
                     KTYMGLFAADRPRADALLIAMKGFVASRRANAAGLEAAQLDAFAKWVDEREVIHTQTA
                     ALTSQTSSFRSW"
     gene            1113..2129
                     /locus_tag="VNN25_23480"
     CDS             1113..2129
                     /locus_tag="VNN25_23480"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXH94560.1"
                     /translation="MKLRSRNRFKHLLPSFAAAAFIVAAACSSGNQPAPATFTWRLPP
                     GFPLPLTPPENLQTDAKFALGRRLFYDRRLSANGTQSCSDCHQQSHAFSDGRPTAIGS
                     TGQVHSRNAMTLTNAGYNASYTWDSVRVRSLEQQALVPMFNTHPIELGVDGHQAEIVA
                     RFRDDPPTAAMFRAAFPGERRPVSIRNIANALATFERALISGNSPYDRLVYLDDRDAL
                     SPSAWRGMRLFFTPRAGCSECHQGFNFSGESRYAGHPNTKPVLVRNGVTDGAFRVPTL
                     RNIELTAPYMHDGSIGTLDEVIARYVTARKLTLSAEEQADLAEFLRSLTDREFVADAR
                     FAAP"
BASE COUNT          433 a          786 c          625 g          391 t
ORIGIN      
        1 gaaccgcgcc tcatccgctg ccatgaccac cagctcgtca cctcggaact caatgctggc
       61 gctctggctg gacacgaaaa gctttgagcg gtgtctccgt ccagagccgg aacacatgac
      121 gatggggccg ccgtcgttga ccaccacgcc gatcgtgtgc gcgtcgaccg taccgatcac
      181 aaccttttga tcatcgcagc gtgaatcgat aaccaaaggc ctggccccaa tcgaaccagg
      241 ccgctaaccg aaggtctggc cccactccca gtcgaacgaa ccttgatcgt cgaagcgttg
      301 agtcgataac caaaggtctg gccccactcc gatgcaccct ccgatgaccc accccgaacc
      361 ctgacaccga ttgctcacgc cactctgccc gccgaggttc ataattaccg cgggaggaag
      421 tccatgaaac gagcaatcat caccgccctt tgcgtcgccg ttctcgccgt cccgtcgctc
      481 atcgcctcgg ggtcgagcgg agggggaatg ggctcggcgc cgtcgccatc gatgcagcag
      541 cagcggtcgc cgcacgatat ggcggtcgac tactacaacg ccgccgagcg ccgtctcgat
      601 ggtctcgcca aaacgcacga cgagatgaag gcctctaccg acgcgcagaa agtcgccaag
      661 ctccaggcga aaatcgccaa agggctcgag aacgccgccg ccgatttcga gcgggccgtg
      721 aagaacgatg cgactctcta ccaggcctac agcgagctcg gattcacgct gcgcaagatg
      781 ggcaagttcg atgagtcgct cgctgcctac gacaagtcct tgtccatcca gcccgactac
      841 actcccgcgc tcgagtaccg cgccgaggcc tacctcgggc tgaacagaat cgacgacgcg
      901 cgcaagacct acatggggct gttcgccgcc gaccgtccgc gcgccgatgc attgctcatc
      961 gcgatgaaag gcttcgtcgc ctcacgccgc gccaatgccg ccggcctcga agccgcgcag
     1021 ctcgatgcgt tcgcgaaatg ggtcgacgag cgcgaagtca tccacaccca gaccgcggcg
     1081 ctcacgtccc agacttccag cttccgctcg tggtgaaact gcgttctcgc aatcggttca
     1141 agcatctcct gccttccttc gccgcggcgg cgttcatcgt cgccgcggca tgcagcagcg
     1201 gaaatcaacc cgcgcctgca accttcacct ggcgccttcc gccaggcttt ccccttccgc
     1261 tgacgccgcc ggaaaatctt cagacggatg cgaagttcgc cctcggacgc cgtctcttct
     1321 acgaccggcg tctctccgcg aacggaacgc aatcgtgcag cgattgccat cagcagagcc
     1381 acgcgttcag cgatgggcgt cccactgcca tcggatcgac cggccaagtg cattcgcgca
     1441 acgccatgac gctgacgaac gccggctaca acgccagcta cacatgggac agcgtccgcg
     1501 tgcgctcgct cgaacagcag gccctcgtgc cgatgttcaa cacgcatcca atcgagctgg
     1561 gcgtcgatgg acaccaggcc gagatcgtgg cgcgattccg tgacgatccc ccgactgccg
     1621 cgatgttccg cgccgccttt ccgggcgaac ggcggccagt ttcgatccgc aatatcgcca
     1681 acgcccttgc cacattcgag cgtgcgttga tctccggcaa ctcgccctac gatcgtctcg
     1741 tctacctcga cgatcgggat gccttgtcgc cgtccgcctg gcgcggcatg cgtctcttct
     1801 tcacccctcg cgccggatgt tcggagtgtc atcaaggctt caatttctcg ggcgaatcgc
     1861 gctacgccgg ccatccgaac accaagcccg tcctcgtgcg caacggcgtg acggacgggg
     1921 cgttccgcgt gccgacgctg cgcaatatcg agctgaccgc accgtacatg cacgacggca
     1981 gcatcggcac actcgatgaa gtgatcgcgc gctacgtgac ggcgcggaag ctcacactga
     2041 gcgccgaaga gcaagcggat ctcgccgagt ttctccgttc gctaaccgat cgggagttcg
     2101 tggcggatgc tcggttcgcg gcaccttgaa aagagcacag acaagagtgt ctgtgccaca
     2161 taaaacccgt tcttagccat cgttcggccc tcgccagaag cgcgggcctg tgtggcacag
     2221 acactcttgt ctgtg
//
LOCUS       DATLVW010000803         4548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_368019, whole genome shotgun sequence.
ACCESSION   DATLVW010000803 DATLVW010000000
VERSION     DATLVW010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368019"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1906)
                     /gene="aspS"
                     /locus_tag="VNN25_23485"
     CDS             complement(164..1906)
                     /gene="aspS"
                     /locus_tag="VNN25_23485"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXH94561.1"
                     /translation="MTRTYAGTLDKQQANQAVTIAGWVQKHRDFGELVFIDVRDRSGI
                     VQVVVDRSKGASEELLAAAKELRSEYVVRIAGEVRERSEQTKNPKMPTGEVELIASSI
                     ELLSRSETPPFPIDDEVDAAEDLRLKYRYLDLRRPLLTRNLILRDRIAHGVRDYMHRQ
                     GFLEIETPILTKSTPEGARDYLVPSRVSKGNFYALPQSPQIFKQLLMVGGLDRYYQIA
                     RCFRDEDLRRDRQPEFTQIDIEASFIDEEFVFGLIEGMFAEVFPLAGIPITTPFPRMR
                     WQEAMERFGIDRPDTRFGMELIDITAAARQLEFVPFREAETVRGIVVPGGAALSRKRI
                     DDLTEEAKKLGASGLVWIKFDAQRGSSIKKFLDDAAFDSMRGALQANEGDMALIVAGK
                     TTRAWDVLGSLRLRVAKERGLIPADRWDFLWVIDFPLFEWDEESGRFFARHHPFTSPR
                     VADADIIESDPGATLARAYDVVMNGLELGGGSIRINTPELQSRMFRALGITDDEARER
                     FGFLLEAFRYGAPPHGGVALGFDRMVMLMARAESLREVIAFPKTARAQDLMSEAPGRV
                     DEKQLGELGIGIKG"
     gene            complement(1985..2497)
                     /locus_tag="VNN25_23490"
     CDS             complement(1985..2497)
                     /locus_tag="VNN25_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXH94562.1"
                     /translation="MILRPYEGKSPRLGQRVFIAENATLIGDVVLGDDCSIWYGAVIR
                     GDVHSIRIGARTNIQDNCVLHVTNDTHPIEIAEEVTIGHGAIVHGCSIARGALIGMGS
                     RVLDGARVGELALIGAGALVSEGMEIPPRTLAVGVPAKVKRDLRIDEIVRLEQSWRNY
                     VGYKDAYLKS"
     gene            complement(2600..3814)
                     /locus_tag="VNN25_23495"
                     /pseudo
     CDS             complement(2600..3814)
                     /locus_tag="VNN25_23495"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          859 a         1397 c         1463 g          829 t
ORIGIN      
        1 ggcgcgcgca tggatgagcg atgcgacctc gccgaggagt tcgcggccgg cgtaaacggg
       61 ataggaggcc gaggcctgac ggatggtgat gaagggagtc attggaagga tgaaggatga
      121 gggatgaggg atgaagtcgc aacagccttc atcccttccg gcttcatcct ttgatcccga
      181 tccccagctc gcccaactgc ttctcgtcca cccgtcccgg cgcctcgctc atgaggtctt
      241 gcgcgcgcgc ggttttcggg aaggcgatca cttcgcgcag cgattcggcg cgggccatca
      301 gcatgaccat gcggtcgaag ccgagggcga cgccgccgtg gggcggagcg ccgtagcgga
      361 aggcttcgag gaggaagccg aagcgctcgc gggcttcgtc gtcggtgatg ccgagggcgc
      421 ggaacatgcg cgactgcagc tcgggcgtgt tgatgcggat cgaaccgccg ccaagctcca
      481 ggccgttcat gaccacgtca tacgcgcgcg ccagcgtggc gccgggatcg ctctcgatga
      541 tgtcggcatc ggcaacgcgc ggcgaggtga agggatgatg acgcgcgaag aaacggccgc
      601 tctcttcgtc ccactcgaac agcgggaagt cgatgaccca gagaaagtcc cagcggtcgg
      661 cggggatcag tccccgctct ttggcgacgc gaaggcgcag ggatccgagg acgtcccacg
      721 cgcgcgtcgt ctttccagcg acgatgagag ccatgtcgcc ttcattcgct tgcagtgcgc
      781 cgcgcattga gtcgaatgca gcatcgtcga ggaacttctt gatcgacgag ccgcgttgcg
      841 cgtcgaactt gatccagacc agtccggatg ccccgagctt cttcgcttct tcggtcaggt
      901 catcgatgcg ttttcgcgag agcgccgcgc cgccagggac gacaatcccg cgtacggtct
      961 cggcctcgcg gaacgggacg aactcgagtt gcctcgccgc ggcagtgatg tcgatcaact
     1021 ccatcccgaa ccgcgtgtcc ggccggtcga tcccgaagcg ctccatcgct tcctgccagc
     1081 gcatgcgcgg gaacggcgtc gtgatcggaa tgcctgccag cgggaagact tccgcgaaca
     1141 tcccttcgat cagcccgaaa acgaactctt cgtcgatgaa cgaggcttcg atgtcgatct
     1201 gtgtgaactc cggctgccgg tcgcgccgga gatcctcgtc gcggaagcag cgggcgatct
     1261 ggtagtagcg atcgagaccg ccgaccatca gcagctgctt gaagatctgc ggcgattgcg
     1321 gcagcgcgta gaagtttcct ttgctgacgc ggctgggcac gaggtaatcg cgcgcccctt
     1381 ccggcgtcga ctttgtcagg atcggtgtct cgatctcgag gaagccctgc cggtgcatgt
     1441 agtcgcggac gccgtgcgcg atgcggtcgc gcaggatcag gttgcgcgtc aacagcgggc
     1501 gccgcaggtc gagatagcga tacttgaggc gaagatcttc ggcagcgtcg acctcgtcgt
     1561 cgatcggaaa gggcggcgtc tcggaacggc tgagaagctc gatcgacgag gcgatcagct
     1621 ccacctcgcc agtcggcatc ttgggattct tggtctgctc ggagcgctcg cgcacctcgc
     1681 ccgcaatacg gacgacgtat tcggagcgca gctctttggc ggcggcgagc agctcttcgc
     1741 tcgcgccttt ggatcgatcg acgacgacct gcacgatacc gctgcggtcg cgaacgtcaa
     1801 tgaatacgag ctcgccgaag tcgcggtgct tctgcaccca gccagcgatg gtcacggcct
     1861 ggttcgcctg ttgtttgtcg agtgtgcctg cgtaggttcg agtcatagtt ctcattgaag
     1921 gatgaatgct gcaggatgaa ggatgagtgc cggctgatga agttcatcct tcatccttct
     1981 gccctcagct tttcaagtac gcgtctttat accccacgta gttgcgccac gactgctcga
     2041 gccggacgat ttcatcgatg cgcaagtcgc gcttcacctt cgccggaaca ccaaccgcga
     2101 gcgtgcgcgg cgggatctcc atcccctcgc tcacgagcgc gcctgcgccg atgagcgcga
     2161 gctcgccgac gcgcgcaccg tcgagaacac gcgagcccat gccgatcagc gcgccgcgcg
     2221 caatcgaaca cccatgcacg attgccccat gcccgatcgt cacctcctcg gcgatctcga
     2281 tcggatgcgt atcgttcgtc acatgcagca cgcagttgtc ctgaatgttc gtccgcgcac
     2341 cgatccgaat cgaatgaaca tcgccgcgaa tgacggcgcc ataccaaatg gaacagtcat
     2401 cgccgaggac aacgtcgccg atgagcgtgg cgttttcggc gatgaagacg cgttgtccga
     2461 ggcgagggga tttgccttcg taggggcgga gaatcatttg ctggactcta tcaggcgggc
     2521 ggtttgagtt gtcaccacag aggcacaaag gacacaaaga aaacctctgt gacctctgtg
     2581 tccctgtggt gaaacgcttc taacgccaca gccgccaagt ccacgccccc caaacgatcg
     2641 cccaaaggat cgccaaagag gccacgccaa ccgtggcgat catcggaccg aaacgatcgg
     2701 atgcgatccc cgcagccagg cttgagatgc tcatcgtcag cgtgacgaag ccccagtcgg
     2761 cggcgaatac gcggccgcga atccagtcgg gtgtctcgcg ctgaagtccg tacgtcgatg
     2821 tctgccactg cgcgccgccg ccgaggtgcg cggcgaagac ggcgagggaa ccgacgagga
     2881 gtgagcgtga gagcccgaga gccatgtagc cgcctccgaa caggagcact gcgaatccga
     2941 tcaggcggta ttgcagatca tcgcgccgcg cgccgactgc gcgaaggatg aacggcccga
     3001 acagcgcacc gagtccgcgc gcggcgaaga gaaggccgat accgaacgcg ccggcgcgga
     3061 agatctcctt gccgaagatg ctgagcatgg cgatgacgcc cgcgccgagt ccgtagccgc
     3121 ctttcgacgt cagaagggca agaacgcgcg gattgccgcg cgcgtaacgc agcgtttcgc
     3181 gaatcgactc cagcagcggc ggatgatcct gatgccgctc ctcggcgaac cgcgagcgca
     3241 tgcgccaaag aaaggcggcc gaagcgagga agctgatgcc gtcggcaacg aaagagacat
     3301 cgcgtccgaa gcgcatcgtc accgctcccc caatcgccgc gccgacggca agcatggtcc
     3361 cccacgtcga tcccatcagc acgttcgcag cactgagatc ctccggcgcg acgatgttcg
     3421 gcagcgctgc ttgcgacgcc ggctcgaagt acgcggagcc gatggagatg atgacgacgc
     3481 cggcgtatgc gaagggcagc atcgcgggcg tccgcgcgaa gagcggaagc agacaggcca
     3541 ccgcgcgaat cagatcgacg gcgatcatca gcttgcggcg atccatccga tcgacggcac
     3601 gccccgcgat cggcgtggcg agaaagatgg gcagggtctg gcagagcagc atcagcgagg
     3661 ccagcgtggc cgagccggtc agttgcagaa cgagatcgag cagcgcaacg gtgaggaacc
     3721 agtcgccgcc gagcgagatg agcgatccgg cgtagagcag ccggaagtcg cggttgtcgc
     3781 gcagaaggcg ccagtacatg cggcgcgcat ggtactgccg ccgggccggc ggcggaccag
     3841 ccgacgagcc gtcggcgcta cgctgttctt accctcctca gcgattgcgc gctcttcctg
     3901 gccgatacgg tcgtaagtca ggaatgagtt tacgtaggtc ggactctcta gtccgacttt
     3961 gcttttgatg tcggactgga gagtccgacc tacgtagatc tcgtcttcac ccgtcttccg
     4021 cgattgcgtg ctcttcgggg ccgatgcggt cgtaggtcag gaatgagttt acgtaggtcg
     4081 gactctttaa tccgacctgc tcgtgatgtc ggactggaga gtccgaccta cgtagatctc
     4141 gtcttcaccc gtcttcagcg atcgcgtgct cttcggggcc gatgcggtcg tagatcagga
     4201 acgagtttac gtaggtcgga ctctctagtc cgacttgctc gtgatgtcgg actagagagt
     4261 ccgacctacg tagatctcgt cttcacccgt cctcggcgat tgcgtgctct tcgggcccga
     4321 tacggtcgta ggtcaggaac gagtttacgt aggtcggact ctttagtccg acttgttcgt
     4381 gatgtcggac tggagagtcc gacctacgta gatctcgcct tcacccgtct tccgcgattg
     4441 cgtgttcttc cgggccgatg cggttgtagt tcaggaacga gtctacgtgg gtcggactct
     4501 ctagtccgac ttgctcgtga tgtcggactg gagagtccga cctacgta
//
LOCUS       DATLVW010000804        24656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_486901, whole genome shotgun sequence.
ACCESSION   DATLVW010000804 DATLVW010000000
VERSION     DATLVW010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24656
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486901"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VNN25_23500"
     CDS             complement(<1..770)
                     /locus_tag="VNN25_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH94563.1"
                     /translation="MFGRVGRERVVGAAWGTLFTSILAILIVVGSRNLQHFDAALVGY
                     TFAVLFATFAITYRYAMWLLRPPTKRYWNRGWQLFFSRRYFVANVGNWFRRVTADFAL
                     NRFIFKRNQSRGVAHFFIMWGCILAFAITFPLVFGWIHFETLPGDLDSYRTFVFGFAT
                     TSFKIHSAVAFLIFHGLVWSAFMVIAGVMLAMRRRMLDHGAAALQRFAEDFLPLVLLF
                     AISITGLLLTASYTWMQGYGYDFLALLHAITVIFTLLYL"
     gene            complement(861..1541)
                     /locus_tag="VNN25_23505"
     CDS             complement(861..1541)
                     /locus_tag="VNN25_23505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH94564.1"
                     /translation="MGIAPVIPFNTIPLLAGIRPADRGALEPLCLMRRYEKGETIFYE
                     SEPAERIHFVVAGRVKIVKATGAREIILEILGPGEPVGAVAVFERRAFPATAVVMEDS
                     TILSIPEREFFQLLESRPEMTRHLLSGLTMRLMMMNKRLADMTGSVEVRAARLFITLS
                     ERMGIPREEQSFIPLALSRQELADLLGTTLETTIRLMSRWQKENLVLTEREGFVVPSV
                     QALRALEP"
     gene            1802..2443
                     /locus_tag="VNN25_23510"
     CDS             1802..2443
                     /locus_tag="VNN25_23510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH94565.1"
                     /translation="MMVALIVAGVLFLLLAFVLFIVAIVAFFMSRNRSNAAVAPPPYV
                     PQPTPAPRVAQPVSVPASPVNAAAPMQPMPAVPAQPAPAAPVQPMAGSYLPPSHVDDD
                     KTVMTDQRRGQLFGALHGTSGPLAGRVFPIDASGFYIGRDRSTSQVVIDSPSISKRHL
                     WIGLKEGVVVAIDQGSTNGTFLNDPNAAITEARLTPGDTLILGGDVARFEYRL"
     gene            2454..3131
                     /locus_tag="VNN25_23515"
     CDS             2454..3131
                     /locus_tag="VNN25_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94566.1"
                     /translation="MTATPKSPWVLAGCGCLSFILALILFVAAYFAGGSHLPSLTSGS
                     STDDTVVYRNTREGRTGKLAENYTAFEFRYPKTWVLKPDPDAANFVTVERAVDGKTYE
                     NLNVGYFSTAGSTEKNQLLYPTLIAQFQSQFEQQFHDLKKVGEGATKVGAYDGWEGLF
                     TSTTGDDDKKVNVYTRVILLPNADGTKGVTLLMMGSSLCPDLSAAEDLGKKGELPAVL
                     DSFKFAE"
     gene            3256..3495
                     /locus_tag="VNN25_23520"
     CDS             3256..3495
                     /locus_tag="VNN25_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXH94567.1"
                     /translation="MVTVNIHEAKTQLSKLVDQAANGEPFIIAKAGKPLVKVVALDAP
                     TAPRRLDFMAGQIAVPEDFDRMGQEEIETLFGING"
     gene            3492..3878
                     /locus_tag="VNN25_23525"
     CDS             3492..3878
                     /locus_tag="VNN25_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH94568.1"
                     /translation="MKLLLDTQILLWSAGQSTRLSSAARKLINDPDNELLFSAASLWE
                     IAIKNTLGREDFRVEARLLRRNLLENGYTELPITGQHAVNVDGLPRLHKDPFDRLLLA
                     QAYSESVTLVTADAQLARYGGAVLKV"
     gene            complement(3905..4303)
                     /locus_tag="VNN25_23530"
     CDS             complement(3905..4303)
                     /locus_tag="VNN25_23530"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HXH94569.1"
                     /translation="MSELQKEPHWVDMAISRLLRGGVVISISVVIAGLILTFVHHPAY
                     FSSKPALGELTAVAESFPHSLADVWKGLTQMRGQALVAFGLLLLIATPVARVGLSIII
                     FAIEKDRLYVGITVTVLLLLFASFVLGAAG"
     gene            complement(4300..5139)
                     /locus_tag="VNN25_23535"
     CDS             complement(4300..5139)
                     /locus_tag="VNN25_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXH94570.1"
                     /translation="MTPLLFVLVVAAISFGAGFLGSLLGLGGGMIVVPALTLLLHIDI
                     RLAIGASIISVIATSSGAAAAYVRDRLANLRVAMFLELGTTSGAITGAWIAGLLHPRW
                     LYILFGSILGYSALAMLRRKRPIPHPPHAPSALAHCLNLGGSYFDDAHGEEVVYEPAR
                     PMLGLGLMYIAGIVSGLLGIGSGALKVPAMDLAMELPIKVSTATSNFMIGVTAAASAG
                     LYFSRGQIDPFIAAPVAIGVVAGAFLGSKFLARISRRAVRTTFVIVLLLVSLQMLVRG
                     VWW"
     gene            5327..6712
                     /locus_tag="VNN25_23540"
     CDS             5327..6712
                     /locus_tag="VNN25_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXH94571.1"
                     /translation="MADEILGCERSESAAALIAVSAVLESLGRALICLDREFRVVHTS
                     ARLGEMIGADAVRSYLGHPVAQLLGSELFAADGALRDAMERGERREGWRASMALPDGT
                     TRLVSCSAAPFRHDDRGICDPNVAYIIIVRPAEDDPSTGTASPTGFAGMIARSSAMTR
                     LFHLIDNLQTSDATILLTGESGTGKEVLAHAIHANSPRKEARFVAVNCGALPADLLEA
                     ELFGHVRGAFTGAIRDRIGRVELASGGTLFLDEIGDLPLQLQVKFLRFLQEKTFERVG
                     DSHTRKADVRIIAATNVDLRRAINEGRFREDLFYRLRVVPIEIPPLRARREDIEPLAR
                     HLLVRVAGQHGRELRFSPDTIRAMLRYSWPGNVRELENAIEYAVAVGRGQTIQPEDLP
                     QEILESPPASRKTSSEGATSTASQAGETRSLRAALEEHHWNREATAHALGIGRTTLWR
                     KMRECGLADGV"
     gene            6852..8099
                     /locus_tag="VNN25_23545"
     CDS             6852..8099
                     /locus_tag="VNN25_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH94572.1"
                     /translation="MKNLVILGAGTGGTIVANGAAKRLPKDWSVTVIDPDVQHLYQPG
                     LLFLPFGIYDESKLWRKRAQTLQKRVQWLQARIDSVDVDRKEVIAGSTRVPYDLLVVA
                     SGSRIRPDLTEGMLEGDWQKRAFDFYTFQGAKKLRQALSSFTEGRLVVNVVDMPIKCP
                     VAPLEFLFLADAHFRNRGIRDRVDLVYATPLDSAFTKPACSRVLGSILDEHGIRLETE
                     FNAGAVDSEKHVLRSWDEREISYDLLVTIPTHSGAAFIEASALGNDSGFVPTDKQTLL
                     AKGHSDIFVIGDATDVPASKAGSVAHFEGEILLENLVQVARGESPSHSFDGHTNCSIE
                     TGDRKAILIDFNYDVEPLPGTYPLPVVGPMALLKESVINHIGKLAFRWLYWNILLPAR
                     PFPVSNRMSMAGKHALIPAPKPA"
     gene            8144..8461
                     /locus_tag="VNN25_23550"
     CDS             8144..8461
                     /locus_tag="VNN25_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HXH94573.1"
                     /translation="MPLRTYAGQSLSVNEEGFLSDSAQWTPAVAEEIAKESGLIPLKE
                     KHWQVITFCREDAAREGQPPGVRRIAKLSGVGMKELYELFPKGPGKLAARIAGLPKPT
                     GCI"
     gene            8534..9007
                     /locus_tag="VNN25_23555"
     CDS             8534..9007
                     /locus_tag="VNN25_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HXH94574.1"
                     /translation="MTAKPPIQAVSIILSKGSLDMVYPALILANGARMSGIDATIFFT
                     FWGLDAITEKKLDNLHVATVGNPAFPIPTMLGGLPGMESLASAFMKRGIEKLDIPPIR
                     EMLEILKDSGAKFYACRMAMEMFNLKQTDLIPQVDGVLSVIDFFDNAAGSQLLFI"
     gene            9023..10270
                     /locus_tag="VNN25_23560"
     CDS             9023..10270
                     /locus_tag="VNN25_23560"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HXH94575.1"
                     /translation="MKRLLIIVALLAASPLFATNGYFLHGIGTGSKAMAGATVALPLE
                     ALDADTNPALGVFVDRGHSFSLALFNPDRQYTITGNPSGMPGTFGLTPGTVASKSNYF
                     PMPAAGFNFRPSPISALTVNFTGHGGMNTDYRTNTFYGSNHTGVDLMQVFLTTTYSRK
                     ITNNQSFGISAVIAEQRFKASGLEAFAAFSSDPDCLTGNGYKWSHGVGIKAGYFAQVL
                     PRLSFGASYSPTITMTKFGKYCGLFAQDGRFNIPASGTAGVAYKINDPLTVTADYQRI
                     HYSDVASVGNALLPNLMTSPLGADGGAGFGWKDINVYKVGMQWKANEVWTLRAGYSKA
                     DQPIPQSEVLFNILAPGVVQQHITLGFSKAMERTPGRFNVAFMYAPSKTVSGPNPLEV
                     PGGQKIALKMHEYELEFGYSFGF"
     gene            10592..12511
                     /locus_tag="VNN25_23565"
     CDS             10592..12511
                     /locus_tag="VNN25_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94576.1"
                     /translation="MSRILLTSVCRPLGERYGDAPSVGYELLFGQVTRKQGLFSPRAN
                     HVQFSLEYIAENLQAPTTVLHYPSRRELIAELRKHPQYVGISFVLSTFHRMKEAAALV
                     RRYSPDSKIILGGYGTVLSNEELAPWSDFICREEGALFMQRLLGEREAPQRHPVVISR
                     LRVFNREVSRTGMIFGGLGCPNGCDFCCTSHFFKRKHIRLLDTGRDIYRVIERYQEIE
                     PEMSMVILDEDFLLNRSRALELRDCVVAGGKALSIFAFASIKAISQYTVTEILEMGID
                     GLWIGYEGTRSGYAKQTGRPVAELFRELREHGISVLASMIVGLPYQTPEVIEEELSGL
                     LDLHPTLSQFLIYGPTPGTPFYEQVMSENRLHRDLVDDRQTYYRNCTGFKAMVTHPSM
                     SPAEIETAQEHCFSEDLRRLGPSMFRSVETWLLGYHRLKDSENPNLRRKAERFAFEIR
                     RAYPIFLAGKVLARGRETRQRIAALETKVHAVLGAPTMSESISSVAALGLAAWTGLTL
                     KMGLFQHPHLTRSTFRVADEPLSSRVWRQLRGADEEGHHVEVELRQGSTVRVSVAGSL
                     SVAGAERLAAELAENLRRRKERLVLDLERIVQAENQAFERVAELLREYAGRIRLVVPD
                     TIDPGPLLAMFALEG"
     gene            complement(12592..13818)
                     /locus_tag="VNN25_23570"
     CDS             complement(12592..13818)
                     /locus_tag="VNN25_23570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH94577.1"
                     /translation="MEETLPVLDRRTGQRHAVALGLVLLFAAAIRIYWNDVEHFFPRS
                     DEGLYLYETRVLVDGGVAAYPGMVDGYLANPARWLYPPPLRWGYLGAAALLCKLSGEI
                     SFRMLATLSAIAGLLSVLLTYLIGAEVIDRTGAVIAAALSATSPLQLALGRRALADEL
                     FCVMFLVTIFLLLRYLRAERPRERAAWLIGWVAAATLTFAVKELFLMVYPIVLLFWWL
                     RAGRRLTLGSIVIWILPPLLFATVFSLLSRSVIKFFQIARILTSAMDASYVHQFQSGP
                     PQRILIDFLAIAPIVTVATIVAAAFVLLRRAEHETGEWHLLLLLAAIAGAHALLPSKN
                     LRYMIEADSLARILAASFFWREIRNRGWGTMAAVTGLAINGAVELMLFITIFVRSEVY
                     DPVTDALLRALEMLPR"
     gene            complement(14027..14797)
                     /locus_tag="VNN25_23575"
     CDS             complement(14027..14797)
                     /locus_tag="VNN25_23575"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXH94578.1"
                     /translation="MRRILIGFVFALSVHAAVAGVNKETIESGGRKHVCYVYAPERLP
                     DAPVPLLMLFHGSGRDGMSQINEWRTLADHERILLAAPDSVDTRQWSMSDDGPDLFRD
                     TLAFVAKQHKIDLRRVYAFGHSAGAAYMLSMAPLESNFFAAIAVHAGAFKNAAASGML
                     ELARRKIPIFIVIGTRDQFFPIEDVRATREAFAGAGFPIETREIPNHDHDYYRSSREI
                     NGMVWTFLSPKHLDADAHYTAYRIVKVGDRVSIEPVEP"
     gene            15002..16120
                     /gene="had"
                     /locus_tag="VNN25_23580"
     CDS             15002..16120
                     /gene="had"
                     /locus_tag="VNN25_23580"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HXH94579.1"
                     /translation="MTTISGISGFRLTAPGGPFRLDEMAPLSAADDEVVVEVAGCGLC
                     HTDVGFAYGGVPTRHPLPLILGHEISGRVVDAGARAATWLGRSVIVPAVIPCDTCEAC
                     LAGRPTICRNQFMPGNDGDGGFATHVVVPARGLCAVPDHLPDGITLEMLSVVADAVTT
                     PYAAMLRASVNEDDLVVIVGAGGIGGFGVQIAAAYGATVVAIDVDDDRLALASVHGAT
                     LTMNPKQLELKAMKSSIRGLARESGRGRIGTKIFEMSGSTTGQQTAFGLLDFGSQLAV
                     VGYTPDKVEIRLSNLMAFDATARGNWGCAPSNYPNALALVLEGKVKLAPFIDIRPFAE
                     LPELFAAASRHELRRRVIVTPAAVARNVVSAEFERTTA"
     gene            16117..17226
                     /gene="oah"
                     /locus_tag="VNN25_23585"
     CDS             16117..17226
                     /gene="oah"
                     /locus_tag="VNN25_23585"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HXH94580.1"
                     /translation="MNDHNLVSGLVSQAVAVSHRSVLDRDGNVVSGLHNVWITLDNQK
                     ELNSYTTPMLKGLILALREASNDRAAVAVILTGAGTAAFCTGGNTRQYAEEYAGRPGE
                     YRQYMRLFNDAVTSILQCDKPVICRVNGMRVGGGQELGMACDFSIASDLAIFSQAGPK
                     HGSAPDGGSTDFLPLFVGIERAMASATLCQNWSAHQFYWLGGLTTIVPALRVDGAWLA
                     NPLVITHRVIDEFGRTIFGQMKTGPERDAAKALMARGTVDLTLLDDAVADLTTKLLYT
                     MPDCLTKTIESLRKHKLEHWDRNRETNRAWLSLNMMTEANAGFRAFHHGTKQQREVDF
                     ILLRQRLAEGAVWGEELIEEIAPWVPTASKETVEV"
     gene            17223..18494
                     /gene="bcrC"
                     /locus_tag="VNN25_23590"
     CDS             17223..18494
                     /gene="bcrC"
                     /locus_tag="VNN25_23590"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HXH94581.1"
                     /translation="MSDARVAAPSSAFGTFSPAEKRGGEGRSIGGAEREGKGLATLIE
                     RAKDLFEDIDFAVVTAWKARTGRKAIGHMPTYVPRELIHAAGMLAVGITGGGDKLEII
                     RGDAYFQSYICRIPRSTIELALSHRLDMLDGMLFPSICDVIRNLSGMWSMIFPDRYVH
                     YVDVPQNFDTEVGGRFWRRELETLRHDFAQLSCVAIDDEALRASIAVYNENRSVIREL
                     YRARSERPWIFPMSEVYYLLRAGNVLPPEEHTSLLREYLALAPGETSRRDQDRSRVVL
                     AGMFCEQPPHGLVMTLERAGCYVVDDDFLLGLRWLQDDVATTGDPLDALVDAYLNHSA
                     MSASRYAPDGDRGRWLIDSVERNRAEGVIFAAASFCDPALLDQPMLVAALDRSSIPHT
                     EFKFAEDTGQFAVIREQAGTFSDSIRLWSEA"
     gene            18491..19765
                     /gene="bcrB"
                     /locus_tag="VNN25_23595"
     CDS             18491..19765
                     /gene="bcrB"
                     /locus_tag="VNN25_23595"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HXH94582.1"
                     /translation="MSAIRKEDSMLLQKQMISDHYDRLASAHDEGQKVVYTFVPGNLN
                     ELVRSFGLLPVLPEINALQSGLRKKSGEYIAEAEKHGHSEDVCTYVKCDIGMLKKGNI
                     SPTGTKLPKPDLLLLSYTGCFTFLKWFELLREEYGDVPVAMLHVPYQANGRIEPHQRD
                     YVVQQMRSEIIPKLEQVSGRKFDEDRLRDMLRLSAAAEDDLVWTLESAKKTPSPIDAY
                     FGGVHYIGPIFSAFRGTQAAVDYYTALRAEVQERLDNRQGPVTPEGPMGKEKYRLVVE
                     GPPNWTSFREFWKMFYDEGAVFVSSTYTKVGGLYDRGFRHDPDHPLETLADYCLGCYT
                     NLGLPTRVDLLEQYIADYEADGFLVNSVKSCNSFSVGQLMMLRQLETRTGVPGLFIEN
                     DLVDPRYFSAANIKNRIESYLQMLDQKRGVTA"
     gene            19765..21063
                     /locus_tag="VNN25_23600"
     CDS             19765..21063
                     /locus_tag="VNN25_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXH94583.1"
                     /translation="MDVFVGIDLGSTTTKAIVLDGDGQVIGRGITNSRSNYDVAALIV
                     REEALVRTRFTLVERSIGADAFVVAALERNLKLEQHLELLARLREAMAMIAAGPKHRA
                     HASDLRERLDEIDAGMEPLARQHFAPGATRRSDFFRDLAAADYMHLAEQLADPKEIAF
                     DVLCGVFDAAILAVENLPIERAFERHIGAALETTLAEHLSCDAAQLRKAVEDARNEDL
                     VERATVGTGYGRARLPFPKEQIRSEILCHGLGAHAMFPATRTVLDIGGQDTKAIQVDA
                     TGIVTSFQMNDRCAAGCGRYLGYIADEMNLALHELGPLAQESRTRVKINSTCTVFAGA
                     ELRERLSLGQKREDILAGLHRAIILRAMSLLARSGGITDQLTFTGGVARNPAAVAALR
                     DLIQENYGVRTLNTSPDSIYTGSLGAALFAQRAAERKAAA"
     gene            21060..21875
                     /gene="bcrD"
                     /locus_tag="VNN25_23605"
     CDS             21060..21875
                     /gene="bcrD"
                     /locus_tag="VNN25_23605"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HXH94584.1"
                     /translation="MIVTAGMDVGSSSVKSAVVVDGEPIALRTDRIRRRELRKVIEES
                     YEAVLADAGVQRSDVVYLATTGEGELVDFRRGHFYGMTTHARGAVFLMPGTEAALDIG
                     ALHARAMKFDAKSRVLAYRMTNQCASGSGQFLENVSRYLGITLEDIGPLSCSASDPEL
                     VSSICAVLAETDVINMVSRGIKTQDILKGIHVSMAGRYLKLLRAIGAEGKVAITGGLS
                     ADTGLAKALQEKVVEEKFAMEIVTDARAIYAGAIGAALWGAFRHNVLARRSAA"
     gene            21906..22595
                     /locus_tag="VNN25_23610"
     CDS             21906..22595
                     /locus_tag="VNN25_23610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXH94585.1"
                     /translation="MSEVAVVTGGSGGIGLAIMSALRATGATVVSLDLAPASTGDWLS
                     CDVRNDTSVAQAIEKVATTHGRIDVAVHAAGITRDAVVWKLGIDEWDIVQEVNLRGAF
                     YLMRHVIPVMRRGAAGSIVLIGSINGSRGKFGASAYAASKAGLIGLAKSVSSETGRFG
                     IRVNVIEPGWVRTPLTDSLPGTLRQQAVNETLLGELVEPDDIAAAVVYLAGAGRRMTG
                     QVLRVDAGQYV"
     gene            22651..22881
                     /locus_tag="VNN25_23615"
     CDS             22651..22881
                     /locus_tag="VNN25_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HXH94586.1"
                     /translation="MNRDLTITVDEEVYEGLHQVIGQQRISQFIEDLVRPHVIHADLS
                     EAYRLMAADETREAEAHEWAEGTFRDVADEAR"
     gene            22868..23191
                     /locus_tag="VNN25_23620"
                     /pseudo
     CDS             22868..23191
                     /locus_tag="VNN25_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004668271.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(23345..24280)
                     /locus_tag="VNN25_23625"
     CDS             complement(23345..24280)
                     /locus_tag="VNN25_23625"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HXH94587.1"
                     /translation="MKKHSLLLMFVLVQIVFAGLLFGEDVRASVANAPSQSGIAKAPV
                     ATSLTPTPAGSRRHAGTPPKIEPEQQFEGTITVASATSITVHDSHGNDVVFALTSATS
                     IRKGDVTVAAADLKVGDQVHVMATSANNVNTALQIVVQTPDAQKEPGPELEIEGIITA
                     ASATEITVHDSHNADDVVELTSTTIIRKGETTVAATDLKVGERVHVKATSANNVNTAV
                     EVIVQDEQQHPLEVTGTITAASSSSITVHDAQGADVILMLTADTIISKGGTTLAAGDL
                     KVGDHVEAEAIFDGTVNNAISIHVDGSSDSGHGHH"
     gene            complement(24436..>24656)
                     /locus_tag="VNN25_23630"
     CDS             complement(24436..>24656)
                     /locus_tag="VNN25_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94588.1"
                     /translation="IMYSLKLEYYRTIPTLSEYIIVSHRERRIVVETRGNDGAWTTRV
                     AAKGERIEVSSLDAELVVDEVYRHSSVR"
BASE COUNT         4895 a         7907 c         7452 g         4402 t
ORIGIN      
        1 agatagagca gcgtgaagat gacggtgatc gcgtgcagca gcgcgagaaa gtcgtagccg
       61 tagccctgca tccacgtgta gctcgccgtg agcagcaacc cggtgatgct gatcgcgaag
      121 agaagcacga gcggcagaaa atcttcagcg aatcgctgca gcgccgccgc tccgtggtcc
      181 agcatccgcc ggcgcatcgc cagcatcacc ccggcgatca ccatgaatgc cgaccacacc
      241 aggccatgaa agatcaggaa ggcaacggcc gaatggatct tgaacgacgt cgtggcgaag
      301 ccaaaaacga acgtgcggta cgagtcgagg tcacccggca gcgtctcgaa atgaatccag
      361 ccgaagacga gcggaaacgt gatcgcgaac gccaggatgc agccccacat gatgaagaag
      421 tgcgcgacgc cacggctctg attccgcttg aagatgaaac ggttgagagc aaagtccgcc
      481 gtgacccggc gaaaccagtt gccgacgttg gcgacgaagt agcgccggct gaagaagagc
      541 tgccagccgc gattccagta gcgtttcgtg ggcggccgca gcaaccacat cgcatagcgg
      601 taggtgatcg cgaacgtcgc aaagaggacg gcgaaggtgt aaccgaccag cgccgcgtcg
      661 aagtgctgga ggttgcgcga gccgacgacg atcaggatcg caaggatcga ggtgaagagc
      721 gttccccacg ctgcccccac cactcgttcc ctgcccacac gaccgaacat gccggcctcc
      781 tgatgccagc atatgctcgg cgatcgtcga tgccatgacg gatgtcatag gccgctgtct
      841 cacgggaagc gatcgcgacc ttacggctcg agagcacgca gcgcttgcac gctcggcacc
      901 acgaagccct cgcgctcggt caacacgaga ttctctttct gccagcggct catgagacgg
      961 atcgtcgtct ccagagtagt tccgagcaga tcggcgagct cctgccggga cagcgccagc
     1021 ggaatgaagc tctgttcctc gcgcggaatg cccattcgct cgctaagcgt gatgaatagc
     1081 cttgccgcgc gcacctcgac cgagccggtc atgtccgcga ggcgcttgtt catcatcatc
     1141 aagcgcatgg tcagaccact gaggagatga cgagtcatct cgggccgcga ctccaggagc
     1201 tggaagaact cgcgttcagg gatcgacagg atcgtcgagt cttccatcac caccgcggtg
     1261 gctggaaagg ctctgcgctc gaagacggcg actgcgccca ccggctcacc aggtccaagg
     1321 atctccagaa tgatctcccg cgccccggtc gctttgacga tcttgacgcg cccggccaca
     1381 acgaagtgga tgcgctccgc cggctccgac tcgtagaaga tcgtttcccc tttctcgtac
     1441 cggcgcatga ggcagagcgg ctccagcgcg ccgcggtccg cgggccggat gcccgccagc
     1501 agcggaatgg tgttgaacgg gatgacgggg gcaatgccca cgtcattagt aaatcatggc
     1561 gtgtcgagtg acggatcccc ctcgcggtag taggtcaatt tgaaaaatcg gtcaccaccg
     1621 aggcacagag gtcacagaga tctcctcatg tgttctctgt gtctcggtgg ttgcctttac
     1681 tcggcgacca cgaatcagcc gtccaaattg acccacaaca cgcccgcgac cgcgaaaccg
     1741 agactccgca accgcgttat catgcagcat tggcccaaaa ccgaaatacc gaggatgatc
     1801 gatgatggtt gcgctgatcg tcgctggggt gctgttcctg ctgctcgcgt tcgtcctctt
     1861 catcgtggca atcgtggcct tcttcatgtc ccgcaaccgg tcgaacgccg ccgttgcgcc
     1921 cccgccttac gttccgcagc caacaccagc tccgcgggtt gcgcagccag tttcggtccc
     1981 tgcctctccg gtgaatgccg ctgctcctat gcagccgatg cctgccgttc cggcgcaacc
     2041 agcgccagcc gctcccgttc agccgatggc tggctcctat ctaccgccca gccatgtcga
     2101 tgacgacaaa acggtcatga ccgaccagcg gcgcggccag ctcttcggcg cgctgcacgg
     2161 aacctcgggg ccgctggcgg gccgcgtttt tccgatcgac gcgagcggct tctacatcgg
     2221 acgcgaccgc tccacgtcgc aggtcgtcat cgattcgccg agcatctcga agcgccatct
     2281 ctggatcggc ttgaaggaag gagtcgtcgt cgccatcgac cagggctcga cgaatggcac
     2341 tttcctcaac gatccgaatg cggcgatcac cgaagcgcgg ctcacgccgg gcgacacgct
     2401 gattctcggc ggcgacgtgg cgcggttcga gtaccgactg tgaggcagat ccgatgaccg
     2461 ccacaccaaa atctccctgg gttctcgccg gatgcggctg cctgagtttc atcctcgcgc
     2521 tgatcctgtt tgtggccgcg tatttcgccg gcggctcgca cctgccgagc cttaccagcg
     2581 gatcatcgac ggacgatacc gtcgtctatc gcaacacacg cgaaggccgg acgggcaagc
     2641 tggccgaaaa ctacacggcc ttcgagtttc gttacccgaa gacgtgggtt ctgaagcccg
     2701 atcccgacgc agcgaacttc gtcacggtcg agcgcgccgt ggatggcaag acgtacgaga
     2761 acctcaacgt cggctacttc tccaccgccg gcagcacgga gaagaatcag ttgctctacc
     2821 cgacattgat cgcgcagttc cagagtcagt tcgaacagca atttcacgat ctgaagaaag
     2881 tcggcgaagg tgcgacgaag gttggtgcct acgatggttg ggaaggcctg ttcacctcga
     2941 cgaccggcga cgacgataag aaggtcaacg tctacacgcg ggtgatcctg ctcccgaacg
     3001 cggacggcac gaaaggcgtc accctcctga tgatgggctc gtccctctgc cccgatctca
     3061 gcgccgccga ggacctaggc aaaaagggcg aactgccggc agtgctcgac tcgttcaagt
     3121 ttgcggagtg agggggaggg ttcggggcgt cgcataggtg acgaatcagc tggcggctgt
     3181 ttcgtatgcg cggttgagca gggaacaacg atttgagaga cttagttcta tcaacttagt
     3241 cgaggacaaa cacttatggt taccgtaaac atccatgaag ccaagaccca gctatcaaag
     3301 ctggtcgatc aagccgccaa tggcgagccg ttcatcatcg caaaggcagg caaacccttg
     3361 gtcaaggttg tggcgttgga tgccccaacg gctccgcggc ggctcgactt catggccggc
     3421 cagattgccg tgcctgagga ttttgatcgc atggggcaag aggagatcga aacgctgttc
     3481 gggattaacg ggtgaaactt cttctcgata cccagattct gctgtggtcg gctggtcagt
     3541 ccacgaggct ttcgtcagcg gcgcgtaaat taatcaatga tcctgacaac gaattgctct
     3601 tcagcgccgc cagcttgtgg gagatcgcaa tcaagaacac cctcggtcgt gaggacttcc
     3661 gcgtcgaggc tcggctcctg cgccgcaatc tcctcgaaaa cggttacacc gagttaccga
     3721 taaccggaca acatgccgtt aacgtcgacg gcttgccacg gttgcataaa gatccttttg
     3781 accgcctgct tctcgcccag gcttactcgg aaagcgtcac tctggttacc gccgacgcgc
     3841 aacttgctcg gtacggtggc gctgtactga aggtgtaagc gccgactcag atcactgttc
     3901 gctcttatcc cgccgcgccg agcacgaacg acgcaaacaa cagcagtagc actgtcaccg
     3961 tgatcccgac gtagagccgg tctttttcga tcgcgaagat gatgatcgag aggccgacgc
     4021 gcgcgacggg cgtggcgatc agcaggagca gtccgaacgc taccagcgcc tggccgcgca
     4081 tctgcgtcaa ccctttccac acatcggcca ggctgtgcgg gaacgactcg gccacagccg
     4141 tgagctcgcc gagtgccggc ttcgacgaga agtacgcggg atgatgcacg aaggtgagaa
     4201 tcaggccggc gatcaccacg gagatgctga tgacgacgcc gccgcgcagc aggcgcgaga
     4261 tggccatgtc gacccaatgc ggttccttct ggagctcgct caccaccaca cacctcgcac
     4321 gagcatctgc aacgagacga gaagcagcac gatgacgaac gtggtccgca cggcgcggcg
     4381 gctgatcctc gccagaaact tcgaccctag gaacgcgccg gcgacgaccc cgatcgcgac
     4441 cggcgccgcg atgaacggat cgatctgtcc tcgagagaag tagagtcccg cgcttgcggc
     4501 cgccgtaacg ccgatcatga agttgctggt cgctgtcgaa accttgatcg gcagctccat
     4561 ggccagatcc atcgccggca ccttcagcgc gcccgagccg atgccgagaa gaccgctgac
     4621 gattcctgcg atgtacatga gcccgagtcc gagcattgga cgcgccggct cgtagacgac
     4681 ttcttcgccg tgcgcatcgt cgaagtacga accgccgagg ttgaggcagt gcgcgagggc
     4741 actcggtgca tgtggcggat gcggtatcgg ccgctttctg cgcaacatcg ccagcgctga
     4801 gtagccgagg atcgagccga agagaatgta gagccagcgc gggtggagga gcccggcaat
     4861 ccacgcgcct gtgatcgccc ccgaggttgt gcccaactcg aggaacatgg ccacgcgcag
     4921 attcgcgagc cggtcgcgca catacgccgc agccgcaccg ctcgacgtcg caatcacgga
     4981 gatgatcgaa gcaccgatgg ccagccggat gtcgatgtgc agcagcagcg tgagcgcagg
     5041 aacgacgatc atcccgccgc ccagcccgag cagcgatcca agaaatcccg cgccgaacga
     5101 gatcgcggcg acgacgagga cgaagagcag cggtgtcatg acccgccgat tccgatcacg
     5161 gcgtaatcat ctcacctgac cggatgtcgc tgagccacgc ggcgcaggcc atcgggcaaa
     5221 ggaactgaag gcgaaccggc ggagatcgta ccgggtgaca gcggtcacgt gcggttgtgt
     5281 tcacggtcat cggtgcgcct tcacgccggg acgaagctgg tcatcgatgg ctgacgagat
     5341 cctcgggtgc gagcgtagcg agagcgcggc tgctttgatc gccgtgagcg ccgtattgga
     5401 atcgctcggc cgcgcgctga tttgcctcga tcgcgaattc cgcgtcgtcc acacgtcagc
     5461 aaggctcggc gagatgatcg gggccgacgc ggtgcgctcg tacctcggcc atccggttgc
     5521 gcaactcctg ggcagcgagt tgttcgctgc cgatggcgct ctgcgcgatg ccatggagcg
     5581 aggcgagcgg cgcgaaggat ggcgcgcatc gatggcgctg ccggatggaa cgacgcgact
     5641 cgtttcgtgc agcgccgctc ctttccggca cgacgaccgc ggaatctgcg accccaacgt
     5701 ggcgtacatc atcatcgtga ggccggccga ggacgacccg tcgacgggaa ctgcttcgcc
     5761 tacggggttc gccggcatga tcgcccgctc gtcggcgatg actcgcctgt tccatctcat
     5821 cgacaacctc cagaccagcg acgcgactat tctcctcacg ggcgagagcg gaaccggcaa
     5881 agaggtcctg gcccatgcca tccacgcgaa ctcgccgcgc aaagaggcgc gtttcgtcgc
     5941 cgtcaactgc ggagcgcttc cagccgattt gctcgaggcc gagcttttcg ggcacgtccg
     6001 cggcgccttc accggcgcga ttcgcgaccg catcggacgc gtcgagctgg cgtcgggcgg
     6061 gacgctattt ctcgatgaaa tcggcgatct tccgctgcag ctccaagtca agttcctgcg
     6121 ctttctgcag gagaaaacgt tcgagcgcgt cggcgacagc catacgcgca aagcggatgt
     6181 tcgcatcatc gccgcgacca atgtcgatct gcgccgtgcc atcaacgagg gacggtttcg
     6241 cgaggatttg ttctaccgtc tccgcgtggt tcccatcgag atccctccgt tgcgggcaag
     6301 gcgcgaagac atcgaaccgc tggcccgcca tcttctcgtt cgcgtagccg ggcaacatgg
     6361 acgcgagctc cgcttctctc ccgacaccat ccgggcgatg ttgcgctact cgtggccggg
     6421 caatgtgcgc gagctcgaaa acgccatcga gtacgccgtc gccgtcggcc gcggccagac
     6481 gatacagccc gaggacttgc cgcaggagat tctcgagagt ccgcccgcat cgcggaagac
     6541 atcttccgag ggcgcgacgt ccaccgcttc acaggccggc gaaacgagat cgcttcgcgc
     6601 cgccctcgag gagcaccact ggaaccgcga ggcgaccgca cacgcgctcg gcatcggacg
     6661 cacgaccctg tggcggaaga tgcgcgagtg cggcctcgcg gacggcgttt gaacgtttga
     6721 acaaaacgtt ccgtttggaa cagtgaccgc gatcacccgc acaccgctat ctgcctctcc
     6781 tcaaatgcga tagccggaat acccttcatg gcagtgcggc tgctctttct cctccccgga
     6841 ggtcgcatga catgaagaac ctggtaatcc tcggcgcggg caccggcgga acgattgtgg
     6901 caaacggcgc tgccaaacgc ctgcccaaag attggtcggt gacggtgatc gatcccgacg
     6961 tccagcacct ctaccagccc ggtctcctgt tcctgccctt tggcatctat gacgagtcga
     7021 agctgtggcg caagcgcgct caaacgctgc aaaaaagggt tcagtggctt caggcacgaa
     7081 tcgactccgt cgacgtcgat cgcaaagagg tgatcgcagg ctcgacgcgt gttccttacg
     7141 acctgctggt ggtcgccagc ggctcccgca ttcgccctga cctgaccgaa ggaatgctcg
     7201 agggcgactg gcaaaaacgc gcctttgatt tctacacatt ccagggtgcg aagaaactcc
     7261 gccaggcgct ttcgtcattc accgaggggc ggctggttgt gaacgtggtg gatatgccca
     7321 tcaaatgccc tgttgcgcct ctcgagtttc tcttcctggc ggacgcgcac ttccgcaatc
     7381 gcggtatccg ggatcgcgtg gacctcgttt atgcgacgcc gctcgattcg gcattcacca
     7441 aaccggcgtg ctcgcgcgta ctcggctcga tcctcgacga gcatggcatt cgcctggaga
     7501 cggagttcaa cgccggggcc gtcgacagcg aaaaacatgt ccttcgttcg tgggatgaac
     7561 gcgagatctc gtatgacctt ctggttacga ttccaacgca ctccggcgcc gccttcatcg
     7621 aggcctcggc actcggaaac gactcaggat tcgttccgac cgacaagcag acgctcctcg
     7681 ccaaaggtca ttcagacatc ttcgtgatcg gcgacgccac cgatgtccct gcctccaaag
     7741 cggggtcggt ggcccacttc gagggcgaga tccttctcga gaacctcgtt caggtggcgc
     7801 gcggcgagag cccatcgcac tcgttcgacg gccacacgaa ctgctccatc gagaccggcg
     7861 accggaaggc gatcctgatc gacttcaact acgacgtcga gccgctaccc ggaacgtatc
     7921 cgcttccggt ggtcgggccg atggccttgc tcaaagaaag cgtcatcaac cacatcggca
     7981 agctggcgtt tcggtggctc tactggaaca tccttcttcc ggcgcgtcca tttccagttt
     8041 cgaaccgcat gtcgatggcc ggaaaacatg cgctgattcc cgcacccaaa ccggcgtgat
     8101 cgcaatgctg aatgtcacgg tcgtgacgaa aaggaggtcc ggaatgccgc ttcgaactta
     8161 tgcaggacag agtctctcag tcaatgagga agggttcctc tccgacagcg cccagtggac
     8221 gcccgcggtc gcggaggaga tcgccaaaga gagcggactg attccgctca aagagaagca
     8281 ctggcaagtc atcaccttct gccgcgaaga cgcggcccgc gaaggtcagc cgccgggcgt
     8341 ccggcgcatt gccaagctct ccggcgtcgg gatgaaggag ctgtacgagc tcttcccgaa
     8401 aggtccggga aaactcgccg cgcgaattgc cggcctgccg aaaccaacag gatgcatctg
     8461 aagggagcac accatgacaa cgacgatcct cgagcctcac accgaacctc acatcgcaga
     8521 cctggaaaag caaatgacgg cgaagccgcc cattcaggcg gtcagcatca tcctctccaa
     8581 gggaagcctc gacatggttt atcccgcgct gattctcgcg aacggcgcgc gtatgagcgg
     8641 cattgacgcc acgatcttct tcacgttctg gggcctcgat gccattaccg agaagaagct
     8701 cgacaatctt cacgtcgcca ctgtcggcaa cccggcattt cccatcccca ccatgctcgg
     8761 cggcctgccc gggatggagt cgctcgccag cgcattcatg aagcgcggaa tcgagaagct
     8821 cgacatccct cccattcgcg aaatgctcga gatcctcaag gacagcggcg cgaagttcta
     8881 cgcctgccgc atggctatgg agatgttcaa cctcaagcag acagacctga tcccgcaagt
     8941 ggacggcgtg ttgtcggtca tcgatttctt cgacaacgcc gccggctcac aactgctctt
     9001 catctgagaa aggagaatca ccatgaaacg gcttctcatc atcgtggcgt tgctggcagc
     9061 gtcacctctc ttcgccacga acggctattt ccttcatggc atcggcacgg gcagcaaggc
     9121 catggccggc gccaccgtag cgctccctct ggaagcactc gacgccgata ccaatcctgc
     9181 cctcggcgta ttcgtcgatc gcgggcactc gttctccctg gccctcttca accccgatcg
     9241 ccagtacacg atcacgggca acccgtccgg catgcccgga acgttcggcc tgacaccggg
     9301 aacggtggcc agcaagtcaa actacttccc gatgccggcg gcaggcttca acttccgccc
     9361 cagcccaatc agcgcactaa cggtgaactt caccggccac ggcgggatga acaccgatta
     9421 ccgcacgaac accttctatg gatcgaatca cacaggagtc gatctgatgc aggtgttcct
     9481 cacgaccacc tactcgcgca agatcacgaa caaccagtcg ttcggaatca gcgccgtgat
     9541 cgccgaacag cgtttcaagg ccagcggcct ggaagcgttc gccgcgttct cgtccgatcc
     9601 tgactgcctg accggcaacg gctacaagtg gtcgcatggt gtcggcatca aggccggcta
     9661 ttttgcccag gtgctacccc gtctttcgtt cggcgcgtcg tatagcccga ccatcacgat
     9721 gacgaagttc ggcaagtact gcgggctctt tgcgcaggac ggccgcttca acatcccggc
     9781 atcgggaacg gccggcgtcg cttacaagat caatgacccg ctgaccgtca ccgccgacta
     9841 tcagcgaatt cactacagcg acgtcgcatc ggtcggcaac gcgctgcttc ccaatctcat
     9901 gacctctccg ttgggcgccg atggcggcgc cggcttcgga tggaaggaca ttaacgtcta
     9961 taaggtcggc atgcagtgga aggccaacga agtctggacg ctgcgagccg gttactccaa
    10021 agccgatcag ccgatcccgc agagcgaggt gttgttcaac atcctcgccc ctggcgtcgt
    10081 ccagcaacac atcaccctcg gtttcagcaa agccatggag cgaacacctg gccgcttcaa
    10141 cgtcgccttc atgtacgcgc cgtcgaagac cgtcagcggc ccgaacccgc tcgaagtgcc
    10201 cggcggacag aagatcgcgc tcaagatgca cgagtacgag ctggagttcg gctactcgtt
    10261 cggattctga ttcaacccac gaagttgcca acgacgcgcg gccctttcaa cgggccgcgc
    10321 gttttgttgt gaaccagaga tcagagacga gagatcagag acaagaaacg agagatcagg
    10381 ttgtttgtct ctgatctctg tttgtctctg atctctgatc ttagcgccag ggttggtccg
    10441 ccgatgccgc atgcagagca atccgcacga cctgatctcc gaaacgattc gcgcgggacg
    10501 cagcacactc gacggtgacc gtccgcacgc tctgctgtgg cgcaggcacg cttcaatgcg
    10561 cgctccgcag tcgtcacgtc aggagtcccc catgagtcga atccttctca ccagtgtatg
    10621 cagaccgctc ggagagcgtt acggcgatgc gccgagcgtt ggttacgagt tgctcttcgg
    10681 acaagtcacg aggaaacagg gtctgttcag tccgcgcgca aaccacgtgc agttctcgct
    10741 cgagtacatc gccgagaacc ttcaggcgcc caccaccgtc ctccactacc cgagccgacg
    10801 cgaactgatt gccgaactgc gcaagcatcc ccaatacgtc gggatctcgt ttgtgctgtc
    10861 gacgtttcac cggatgaaag aagcagccgc gctcgtgcga cgctactctc ccgactcgaa
    10921 aatcatcctc ggcggctacg gcacggtgct atccaatgag gagctggcgc catggtcgga
    10981 cttcatctgc cgcgaagagg gagccctctt catgcagcgc ctcctcggcg agcgtgaagc
    11041 tccgcagcgg catccggtcg tcatcagccg gcttcgcgtg ttcaaccggg aagtctcccg
    11101 caccggcatg attttcggcg gcctcggctg cccgaacggc tgcgacttct gctgcacatc
    11161 gcactttttc aagagaaaac acatccgcct tctcgacacc ggccgcgata tctatcgcgt
    11221 gatcgagcgc tatcaggaga tcgagccgga gatgtcgatg gtcatcctgg acgaggactt
    11281 cctcctcaac cgcagtcggg cccttgagct gcgtgactgc gtcgtcgccg gcggcaaggc
    11341 gctctcgatc ttcgccttcg ccagcatcaa ggccatcagc cagtacacgg tcaccgagat
    11401 cctggaaatg ggaatcgacg gcctctggat cggctacgaa gggacgcgtt ccggatacgc
    11461 caagcagacg ggccggcccg tggccgagct atttcgggag ctgcgggagc atggcatcag
    11521 cgttctcgcc tcgatgatcg tcggacttcc gtaccagaca cccgaggtga tcgaagaaga
    11581 gctcagcggg ctgctcgacc tgcaccccac cctctcgcag ttcctcattt acggcccgac
    11641 acccgggacg cccttctacg aacaggtcat gagcgagaat cgtctgcacc gcgatctcgt
    11701 cgatgatcgc cagacctact atcgcaactg caccggcttc aaggccatgg tgacgcatcc
    11761 gtcgatgagc cccgcggaga tcgagaccgc gcaggagcac tgcttctcgg aagacctgcg
    11821 gcgcctggga ccttcgatgt tccgctctgt cgagacctgg ctcctcggct accaccgtct
    11881 caaagactcg gagaacccga acctgcgccg gaaagcggaa cgattcgcct tcgagatccg
    11941 ccgtgcctat ccgattttcc tcgccggaaa ggttctggcg cgcggacggg aaacacggca
    12001 aaggattgcc gcgctcgaaa caaaggtcca tgccgtgctc ggcgctccga ctatgagcga
    12061 gagcatttca tccgttgccg ccctcgggct ggccgcctgg acgggattga cgctgaagat
    12121 gggactcttt cagcacccgc atctcacgcg gagcaccttc cgcgtcgccg acgaaccgct
    12181 ctcttcgcgc gtctggcgcc aactgcgcgg cgcagatgaa gaaggtcatc acgttgaggt
    12241 ggagttgcgg cagggatcga cggtccgcgt ctccgttgcc ggcagcctca gcgtcgccgg
    12301 ggccgaacga ctggccgcgg aactggcgga gaacctgcgc cgccggaagg aacgtctggt
    12361 gctcgacctc gaacgaatcg ttcaggcgga gaaccaggcc ttcgagcgag tggccgagct
    12421 tctccgcgaa tatgccggcc gcatccggct ggtcgttccc gacacgatcg acccggggcc
    12481 gttgctggct atgttcgcgc tggaagggtg aacggctctt caccacaaag acacagagga
    12541 cacagagaag gtcgtctttc tctgtgtcct ctgtgtctct gtggtcaaaa actatcgcgg
    12601 cagcatctcg agcgcccgaa gcaacgcatc ggtcaccggg tcgtagactt cgctccgaac
    12661 gaagatcgtt atgaatagca tcaactcgac ggcgccgttg atggcgaggc ccgttactgc
    12721 agccatcgtc ccccatccgc ggtttcggat ctcccgccag aagaacgatg ccgccaggat
    12781 gcgcgcgagc gagtcggcct cgatcatgta ccgcaggttt ttcgacggca gcaacgcatg
    12841 ggcgccggcg attgctgcca gcagaaggag cagatgccac tccccagttt cgtgctccgc
    12901 gcgtcgcagc agtacgaaag ccgcggcaac gatcgtggct accgtgacga tgggcgcgat
    12961 tgccaggaag tcgatcagga ttcgctgcgg cgggccgctc tggaattgat gcacgtatga
    13021 cgcgtccatc gccgaggtga ggattctggc gatttgaaag aacttgatca cgctacgcga
    13081 gagcagagag aaaaccgtgg cgaacagcag cggcggcagt atccagatca cgatgctccc
    13141 cagcgtgagg cggcgcccgg cgcgcagcca ccagaacagc agcacgatcg gatacaccat
    13201 caggaacagc tctttgaccg caaacgtcaa cgtagccgcg gccacccatc cgatcagcca
    13261 tgccgctcgt tctcgcggcc gctccgcgcg caggtagcgg agcaacagga agatcgtgac
    13321 gagaaacatc acgcagaaca actcgtcggc cagcgcgcgc cggcctagcg ccaactgcag
    13381 gggagacgtt gccgacaacg cagcagcgat gaccgcaccc gtccggtcga tgacttcagc
    13441 gccgatgagg tatgtcagca agaccgacaa cagcccggca atggccgaaa gcgtggccag
    13501 catccgaaaa ctgatctcgc cgctcagttt gcagaggagg gccgccgcac cgagataccc
    13561 ccagcgaagc ggagggggat aaagccatcg cgccggattg gcgagatacc catcgaccat
    13621 gccgggataa gccgcaactc ccccgtcgac gagcacccgc gtttcgtaga ggtaaagccc
    13681 ctcgtccgag cgcggaaaga agtgctcgac gtcgttccag tagatccgga tcgccgccgc
    13741 aaacaaaagg acgagcccca aagccaccgc gtggcgttgc cccgtgcgtc gatcaaggac
    13801 cggcaatgtc tcttccatga atcagtggat cgttgtcgat tcgatttaac cacttcgatg
    13861 ggctcgaagg acaacggcgc ttcgcaaacc ggcctcacgc ctgctcgagc gcgagcgaaa
    13921 atcgatcgcg cccgtacggc ggttcctctt ttggtggcag cggatcgctt ggagatttct
    13981 gtgacatcag aagcgcaaga gcttagtgag ttcgatcact ctctcctcac ggctcgactg
    14041 gctcgatgct gactctgtcc ccaaccttca cgattcgata cgcggtgtag tgagcatccg
    14101 cgtcgaggtg cttcggagaa aggaacgtcc agaccattcc gttgatctcg cgcgagctgc
    14161 ggtaatagtc gtggtcgtga ttcgggatct cgcgagtctc gatcggaaac ccggcgccgg
    14221 caaacgcctc gcgcgtcgcg cggacgtctt cgatcgggaa gaactggtcc ctcgtcccga
    14281 taacgataaa gatcgggatc ttccttcggg ccagctccag catgcccgac gccgcggcgt
    14341 tcttgaacgc cccagcgtgc actgcgatgg ccgcaaagaa attggattcg agcggcgcca
    14401 tgctcagcat gtacgcagca cctgccgagt gcccgaacgc gtagacccgc cttaagtcga
    14461 tcttgtgttg cttcgccaca aacgcgagtg tgtcccgaaa gagatcgggc ccgtcgtccg
    14521 acatggacca ctggcgtgtg tccactgagt cgggagctgc cagcaggatt cgctcgtgat
    14581 cggcaagcgt tcgccactcg ttgatttgcg acatcccgtc gcgccctgag ccgtggaaga
    14641 gcatcaaaag cggcaccgga gcgtccggca atcgctcagg cgcatacaca tagcagacgt
    14701 gcttgcgccc tcccgattcg atggtctcct tgttcacgcc tgctacggcc gcgtgcaccg
    14761 acagcgcaaa gacgaaacca atgagaattc gacgcatgta atccccttgc cggcttagtg
    14821 agcgtgccag tgtatgacgt gacgagttgg acatgcgaac gcttccctgc cgtgttggcg
    14881 acgttataag caattcggtt cactgcccct gtccgcgtca ctctcgccgg tgacccgaat
    14941 cacccctctt agtccgtccc ctcgcctagc ttcgaatcca tcgaattgga gcgacgcgac
    15001 gatgacgacg atctcgggaa tctctggttt tcggcttacg gcgcccggtg ggccgtttcg
    15061 gttggacgag atggcgccgc tgagcgcggc ggatgatgaa gtcgtcgtcg aagtggcggg
    15121 ctgcgggctc tgtcacacag acgtcggctt cgcgtacggc ggcgtcccaa cccgccaccc
    15181 cctcccgctc atcctcgggc atgagatctc cggacgggtc gtcgacgcgg gtgctcgcgc
    15241 ggcgacatgg ctcggccgca gcgtgatcgt gccggccgtg attccgtgcg atacctgcga
    15301 ggcctgcctt gccgggcgcc cgacgatctg ccgcaatcag ttcatgccgg gaaatgatgg
    15361 agacggcggc ttcgccacgc acgtcgtcgt tcccgcgcgc ggactctgcg ccgttcccga
    15421 tcatcttccc gacggcatca ccctggaaat gctctccgtc gttgccgacg ccgtgaccac
    15481 accgtatgcg gcgatgcttc gcgcctcggt caacgaagac gatctggtcg tcatcgtcgg
    15541 cgccggcggt atcggaggat tcggcgtgca gatcgcggcc gcgtacggcg cgacggtcgt
    15601 ggccatcgac gtcgacgacg accgccttgc gctggcaagc gtgcatgggg cgacgctgac
    15661 gatgaatccg aaacagctcg agctgaaggc gatgaagagc tcgatccgcg ggctcgcgcg
    15721 cgagagcgga cgcggacgca tcggcacgaa gatcttcgag atgagcggct caacgactgg
    15781 tcagcagacc gcgttcgggc tgcttgattt cggcagccag ctcgccgtcg tcggctacac
    15841 gccagacaaa gttgagatcc ggctctcgaa tctgatggcc ttcgatgcta cggctcgcgg
    15901 caactggggc tgcgcgccgt cgaactatcc gaatgcgctg gcgctcgtgc tcgaaggaaa
    15961 agtgaagctc gcgccgttca tcgacattcg cccgttcgcc gagctgcccg agctgttcgc
    16021 cgccgcatcg cggcacgagt tgcgccgccg cgtcatcgtc acgccggccg ccgtcgcccg
    16081 aaacgtcgtg agcgcggaat tcgaaaggac cactgcatga acgatcacaa cctcgtttcc
    16141 ggcctcgtgt cgcaggcggt ggccgtttcg caccgctccg tgctcgaccg cgacggtaat
    16201 gtcgtcagcg gcctgcacaa cgtctggatc acgctcgaca atcagaagga gctcaacagc
    16261 tacacgacgc cgatgctgaa ggggttgatc ctcgccctgc gcgaggccag caacgatcgc
    16321 gccgctgtgg ccgtgatcct caccggtgca ggtacggcag cattctgcac cggcggcaac
    16381 acgcgtcagt acgccgagga gtatgcgggg cgtccgggcg aataccggca gtacatgcga
    16441 ctcttcaacg acgccgtcac gtcgatcctg cagtgcgaca agccggtgat ctgccgcgtg
    16501 aacggaatgc gcgtcggcgg cggccaggag ctcggcatgg cctgcgactt ttccatcgcc
    16561 agcgatctgg cgatcttcag tcaggccggg ccgaaacatg gatcggcgcc cgacggcggc
    16621 agcacagact tccttccgct tttcgtcggc atcgagcgcg cgatggccag cgccaccctc
    16681 tgccagaact ggagcgcgca tcagttctac tggttgggcg gtttgacgac catagttccc
    16741 gcactccgcg tcgacggcgc ctggctcgcc aacccgctcg tcatcaccca tcgcgtcatc
    16801 gacgaattcg gacgcaccat cttcggacaa atgaaaaccg gcccggagcg cgatgcggcg
    16861 aaggcgttga tggcccgagg gacggtcgat ctcacgctgc tcgacgatgc ggtcgccgat
    16921 ctgacgacga agcttctcta cacgatgccc gattgcctga cgaagacgat cgagagtctc
    16981 cgcaagcaca agctcgaaca ttgggaccgc aatcgcgaga cgaatcgcgc gtggctgtcg
    17041 ctgaacatga tgaccgaggc taatgcgggg ttccgcgcgt tccatcacgg cacgaagcag
    17101 cagcgcgaag tcgatttcat ccttctgcgg caacgcctgg cggaaggggc ggtatggggc
    17161 gaggaattga tcgaggagat cgcgccgtgg gttcctactg cttcgaagga gacggtggaa
    17221 gtgtgagtga cgcgcgagtg gcagccccct catccgcctt cggcaccttc tcccccgctg
    17281 aaaagcgcgg gggcgaaggg cgctcgattg ggggtgcgga gcgagaggga aaagggctcg
    17341 ccacgctcat tgagcgcgcg aaagacctct tcgaggatat cgacttcgcc gtcgtcaccg
    17401 catggaaggc gcgcacaggg cgcaaggcga tcggccacat gccgacgtac gttccgcgcg
    17461 agctgatcca tgcggccggc atgctcgccg tcggcatcac gggcggcggc gacaagctgg
    17521 agatcatccg cggcgacgcc tactttcaga gctacatctg ccgcatcccg cgcagcacga
    17581 tcgagctggc actgtcgcac cggctggaca tgctcgacgg catgcttttc ccgtcgatct
    17641 gcgacgtcat ccgcaatctg tcgggcatgt ggtcgatgat ctttcccgac cgctacgtgc
    17701 actacgtcga cgtcccgcag aatttcgata ccgaggtcgg cggccggttc tggcgccgcg
    17761 aattggaaac gctgcgccac gatttcgcgc aactctcctg cgtcgccatc gacgacgagg
    17821 cgctgcgcgc gagcatcgcg gtctacaacg agaatcgcag cgtcattcgc gagctttatc
    17881 gcgcacgcag cgagcggccg tggatctttc cgatgtccga ggtctattac cttttgcgcg
    17941 cggggaacgt cttgccgccc gaggagcaca cgtcgctcct gcgcgagtac cttgctcttg
    18001 ctcccggcga gaccagccgg cgcgatcagg accgcagccg cgtcgttctg gccggcatgt
    18061 tctgcgagca gccgccgcac ggtctggtga tgacactcga gcgcgccggc tgttacgtcg
    18121 tcgatgacga tttcctcctg ggccttcgct ggttgcagga tgacgtcgcc acgaccggcg
    18181 atccgctcga tgcactcgtc gatgcgtatc tgaatcacag cgcgatgagc gcctcgcgct
    18241 acgcccccga cggcgaccgc ggacgctggc tgatcgactc cgtcgagcgc aaccgcgccg
    18301 agggcgtcat cttcgccgcg gccagcttct gcgatccggc gctgctcgat cagccgatgc
    18361 tcgttgccgc gctcgatcga agcagcatcc cgcatacgga gttcaagttc gccgaggaca
    18421 ccgggcagtt cgctgtgatc cgcgagcagg ccggcacatt ctccgattcg attcgactgt
    18481 ggagcgaagc atgagcgcca ttcgcaaaga agactcgatg cttctgcaga agcagatgat
    18541 ctccgaccac tacgaccggc tggcgtcggc ccacgatgag ggacagaagg tggtctacac
    18601 gttcgtgccc ggaaatctga acgagctcgt tcgctcgttc ggtctgcttc ccgttcttcc
    18661 cgagatcaac gcgctgcaat caggactgcg caagaaatcc ggcgagtaca tcgccgaggc
    18721 ggagaagcac ggccacagcg aagacgtctg cacatacgtc aagtgcgaca tcggcatgtt
    18781 gaagaagggg aacatcagcc ccacgggcac gaagctgccg aaacccgatc tgctgctcct
    18841 ctcctacacc ggctgtttca cctttctgaa atggttcgag ttgctgcgcg aagagtacgg
    18901 cgacgtgccg gtggcgatgc tgcacgtgcc gtatcaggcc aacggccgca tcgagccgca
    18961 tcagcgcgat tacgtcgtgc agcagatgcg cagcgagatc atcccgaagc tcgagcaggt
    19021 cagcggacga aagttcgatg aggaccgctt gcgcgacatg ctgcgcctct cggcagccgc
    19081 cgaggatgat ctggtgtgga cgctggaaag cgcgaagaaa acaccgtcgc cgatcgatgc
    19141 gtacttcggc ggcgtccact acatcggccc gatcttcagc gcgtttcgcg gaacgcaggc
    19201 cgcggtcgat tactacaccg ccttgcgcgc ggaagtgcag gagcggctcg acaacaggca
    19261 ggggccggtc actcccgaag gtccgatggg gaaggagaaa taccgcctcg tcgtcgaagg
    19321 ccctccgaac tggacgagct ttcgggagtt ctggaagatg ttctacgacg agggcgcggt
    19381 cttcgtctcc agcacgtata cgaaggttgg cggtttgtac gatcgcggtt tccgccacga
    19441 tcccgatcac ccgctggaga cgctcgccga ctactgcctc ggttgctaca ccaacctcgg
    19501 gcttccgacc cgcgtcgacc tgctagagca gtacattgcc gattacgaag cggacggctt
    19561 cctcgtcaac agcgtgaaga gctgcaactc gttcagcgtc ggacaactga tgatgctgcg
    19621 gcaactggag acgcgaaccg gcgtcccggg cctcttcatc gagaacgacc tcgtcgatcc
    19681 gcgctacttc tccgctgcca acatcaagaa ccggatcgag agctatctgc agatgctcga
    19741 tcagaagcgc ggagtgacag catgatcgac gtcttcgtcg gcatcgacct cggctccact
    19801 acaacgaaag caatcgtcct cgacggcgac ggccaggtca tcggccgcgg catcacgaac
    19861 agccgcagca actacgacgt agccgcgctc atcgtgcgcg aagaagcgct cgtacggacg
    19921 cgcttcacgc tcgtcgagcg cagcatcggc gcggatgcct tcgtggtcgc ggcgctggag
    19981 cggaatctca agctggaaca gcatctggag ttgctggccc ggctgcgcga agcgatggcg
    20041 atgatcgccg ccggaccgaa gcaccgcgcg cacgccagcg atcttcgcga gcgcctcgac
    20101 gagatcgacg ccgggatgga gccgctggcg cggcagcact tcgcgcccgg cgcaacgcgc
    20161 aggagcgact tcttccgcga cctcgccgcc gccgactaca tgcacctcgc cgaacaactc
    20221 gccgatccga aagaaatcgc cttcgacgtg ctctgcggtg tgttcgatgc cgcgattctc
    20281 gccgtcgaga atctgcccat cgagcgcgcc ttcgagcgcc acatcggcgc cgcgctcgaa
    20341 acgacgttgg ccgagcatct gagctgcgac gcggcgcaat tgcgcaaagc ggtcgaagat
    20401 gcacgcaacg aagatctcgt cgagcgcgcc accgtcggca ccggttacgg acgcgcacgc
    20461 ctcccgttcc cgaaagaaca gattcgcagc gagatcctct gtcacggcct cggagcacac
    20521 gcgatgtttc cggcgacgcg caccgtgctc gacatcggcg gccaggacac gaaagcaatc
    20581 caggtcgatg ccacagggat cgtcaccagc ttccagatga acgaccgttg cgccgccggg
    20641 tgtggccgct acctcggcta catcgccgat gagatgaatc tcgcactgca tgagctcggg
    20701 ccgctggcgc aggagagccg gacgcgcgtg aagatcaaca gtacctgcac ggtcttcgcc
    20761 ggcgcggagc tgcgcgagcg gctttcgctc gggcagaaac gcgaagacat cctggccggg
    20821 ctgcaccgcg cgatcatcct ccgggcgatg tcgcttctgg cgcgttcggg cggcatcacc
    20881 gatcaactca ccttcaccgg cggcgtggcc aggaatccgg cagccgtcgc cgcgctgcgc
    20941 gatctcattc aggaaaacta cggcgtgcgc acgctgaaca cttcgcccga ttcgatctac
    21001 accggctccc tcggcgcggc cctcttcgcg cagcgcgccg cggaaaggaa agcagcagca
    21061 tgatcgtcac cgcaggcatg gacgtcggat cgagcagcgt gaagagcgcc gtagtcgtcg
    21121 acggtgaacc gatcgcgctg cgcaccgatc gcatccgccg ccgcgagctt cgcaaagtca
    21181 tcgaagaatc gtacgaagcg gttctcgctg acgccggcgt gcagcggagc gacgtcgtct
    21241 acctcgccac caccggcgaa ggggagctgg tcgacttccg ccgcggccac ttctacggca
    21301 tgaccacgca cgcgcgcggc gccgtcttcc tgatgcccgg aaccgaggcg gcactcgaca
    21361 tcggagcgct gcatgcgcgg gcgatgaagt tcgatgcgaa gagccgcgtg ctggcctatc
    21421 gcatgacgaa tcagtgcgcg tccggctccg gccagttcct cgaaaacgtc tcgcgttacc
    21481 tcggcatcac actcgaagac atcggcccgc tttcgtgcag cgcctcggac ccggagctcg
    21541 tcagcagcat ctgcgccgtg ctcgccgaga ctgacgtgat caacatggtc agccgcggca
    21601 tcaagacgca ggacatcctc aaaggcattc atgtctcgat ggcggggcga tacctcaagc
    21661 tgctacgcgc aatcggcgcc gaaggaaaag tcgcgatcac cggcggactc tctgccgaca
    21721 ccggtctggc gaaggcgttg caggagaagg tggtcgaaga gaaattcgcg atggagatcg
    21781 tgaccgatgc gcgcgccatc tacgccggcg cgatcggcgc ggcgctctgg ggcgcgttcc
    21841 ggcacaatgt tctggcgcgc aggagcgcgg catgagaatg agatgttgaa acgcgggagc
    21901 gcacaatgag cgaagttgcc gtcgtcaccg gaggctcggg cggcatcggc ctggccatca
    21961 tgtccgcgct gcgcgccacc ggcgcgacgg tcgtcagcct cgacctcgca ccggcatcga
    22021 ccggcgactg gctctcgtgc gacgtgcgca acgacacctc ggtcgcgcag gcaatcgaaa
    22081 aagtcgccac cacgcacggc cgcatcgacg tcgccgttca tgctgccggc atcacgcgcg
    22141 acgccgtcgt ctggaagctc ggcatcgatg aatgggacat cgtccaggaa gtgaatctgc
    22201 gcggcgcttt ctacctgatg cgccacgtca tcccggtcat gcgccgcggc gcggcaggaa
    22261 gcatcgttct gatcggatcg atcaacggct cccgcggaaa attcggagcg tcggcgtacg
    22321 cggcgagcaa agccggcctg atcggactgg ccaaaagcgt gagcagcgag accggccgct
    22381 tcggcatccg cgtaaacgtc atcgaaccgg gatgggtgcg cacccccttg accgactccc
    22441 ttcccggcac gctccgccaa caagccgtca acgaaaccct cctcggcgag ctcgtcgagc
    22501 ccgacgacat cgccgcggcg gttgtctacc tagcgggcgc cgggcgacga atgaccggtc
    22561 aggtactgcg ggtggacgcc gggcagtacg tgtaacgctt gacgtcgcaa cgcacgggcg
    22621 taacatctac ggagaggacg ttgtacaaag atgaaccgag acctgacgat cacagtggac
    22681 gaagaagtgt acgagggcct gcatcaagtc attgggcagc aacgcattag ccagttcatc
    22741 gaggatctgg tacggcccca cgtgattcat gccgaccttt cggaagccta ccggctcatg
    22801 gctgccgacg aaaccaggga agccgaggct cacgagtggg ctgaaggcac cttccgcgat
    22861 gtcgctgatg aagcgcggtg aggtctggtg ggtcgacttc gacccgtccg taggcggcga
    22921 gattcagaaa cgtcgcccag ccgtaatcgt aagtaacgac gcttccaatc aacacctcaa
    22981 ccgcgtccag gttgttcccc tgaccagcaa tgtcgagcgc ctgtatccca gcgaggcata
    23041 cgtcacattg aatggccaac agcgctaagc catggctgat cagctgacaa cggtcagcaa
    23101 acaacgcctc cagactctga ttggcactct cgccacgccg gatctcgagc tcgtgggcaa
    23161 tgccattcgg ttgcagctgg cgctccccta ggcctcgtca agcaactcca catcaagctt
    23221 caacgctttt tcatcggcac aagaacgagc gggtcgtaat gctacgtaat gctacggcac
    23281 aagaatcgtc cgcccaacag atgctggcgg cgccggtact caacccggcg ccgccatatt
    23341 cgaatcagtg gtgaccgtgg ccgctatcgc tggagccatc cacatgaatg gagatggcgt
    23401 tgttgaccgt accgtcgaag atcgcctcgg cctcgacgtg gtcgcctacc ttcagatcac
    23461 cggcggcgag cgtggtgccg cccttgctga tgatcgtgtc ggcggtcagc atcagaatga
    23521 cgtcggcgcc ctgagcatcg tgaacggtga tcgacgagga ggacgccgcc gtgatggtcc
    23581 cggtgacctc gagcggatgc tgctgctcgt cctgcacgat cacctccacc gcggtgttga
    23641 cgttgttcgc cgacgtggcc ttgacgtgaa cccgttcgcc aactttcaga tcagtcgcgg
    23701 cgacggtcgt ttcgcccttg cgaatgatcg tcgtcgaagt cagttccacg acgtcatcgg
    23761 cattgtgcga atcatgaacg gtgatctcgg tcgccgacgc ggcggtgatg attccttcga
    23821 tttcgagctc gggcccgggc tccttctgcg catcgggagt ctgcaccacg atctgcagcg
    23881 ctgtgttgac gttgttcgcc gacgtggcca tgacgtggac ctgatcgccg accttgagat
    23941 cggccgcggc gacagttacg tcgcctttgc gaatcgacgt cgccgaagtc agagcgaaca
    24001 cgacgtcgtt gccgtgtgag tcatgaacgg tgatcgaggt cgccgaggca acggtgatgg
    24061 ttccttcgaa ctgctgttcc ggctcaattt tcggcggcgt accggcgtgc ctgcgcgacc
    24121 cggccggagt cggagtcagc gatgttgcca cgggagcctt cgcgatccca ctctgcgatg
    24181 gtgcgttagc caccgaggcc cgcacatctt ccccgaacag caaccctgca aagacgatct
    24241 gaaccaggac gaacatcaag agcaaactat gttttttcat gtctcctcca aaatgaaaaa
    24301 ggggcctacg cccaaaaagc aaaaagaggg cgtgggtacg ccctctttag gaactgaact
    24361 tcttctgtcg gctgggatta taacgacgac gcggatgcga ctgtcaagcg agcgcgagcg
    24421 ggcaagttgg tttcctcacc gcacggaact gtggcgatag acctcgtcga cgaccagctc
    24481 ggcatcaaga gaagagacct caattcgctc tcccttcgct gcgacgcgcg tagtccacgc
    24541 gccatcgttt ccgcgcgtct caacgacgat gcgccgctcg cgatgcgaca cgattatgta
    24601 ttcgctcagg gttgggatcg ttcggtagta ctcgagcttc agcgaataca taatcg
//
LOCUS       DATLVW010000805         4802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_368025, whole genome shotgun sequence.
ACCESSION   DATLVW010000805 DATLVW010000000
VERSION     DATLVW010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368025"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(789..2588)
                     /locus_tag="VNN25_23635"
     CDS             complement(789..2588)
                     /locus_tag="VNN25_23635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH94589.1"
                     /translation="MLSPDQLLGPYRLINRVGAGGMGEVWRAEDSRLGRVVAIKVLPP
                     SFVADAEATSRLKREARTAAQLYHPSIATIHSIEQDGDHIFIVEEFVEGDSLTKVIKR
                     GGLAETEICRIARAVADALAEAHAKGIVHRDIKPDNIIVAGNRVKVLDFGIAKQVGAP
                     GASPETFVTQQGMILGTVHYMSPEQALGKTFDGRTDVFSLGVVLYEMATGRKPFEGET
                     ITETMTQIIRDEPVDAAAVNPGVSPGLKAIIDRSLRKNPGERYTAAELVTALDTQLGR
                     AGTVPYTSPQSAAPTVVVAAAAGGDPTVRTGPQVQTAAPTQIQGVAPTIRTGPQAPTV
                     QELAAPQPPAKPKGRGALAAVIVVMVIILAAVAGVWATHRSEPKVTASQSVVPPQTSA
                     AQSSTAVNVTAPSDATVEETASDTSNTTTTAKQVGKTSTTPTTDTAEETQTTAEPTAS
                     APAESESAPGADALYTNAMRQVRDGDAMEARKTLHRVLQQDPHYAKAHFRLGEIALLN
                     RNLDYASQELQKALDDTDRLGPREQQLTHLGMALATHEKREVERLSDEIEQRWPNDPD
                     LERMRTTFPGMFGELGRGVQQRDHGRARRWQRP"
     gene            2643..3533
                     /locus_tag="VNN25_23640"
     CDS             2643..3533
                     /locus_tag="VNN25_23640"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH94590.1"
                     /translation="MSERKPCVRCERPIDSSARSCVYCGWDQARPRPAESTTPPIANP
                     YTPPADNRVRNRLLGAGAFVVLVILAFFVGSLLNRSEGNETSASEKASNPSPQTGAGS
                     SSRTTVMLVPVDGTSPLQEQPPVTSAPAPPASAGSAEINGERSDATALPSGAYSVAVE
                     RARAEREKHPNAGIVDPRTIAGGVESGPARRTASREAQRANAQRAANNIPTESLRTQP
                     VPLYQPVPSMQVYRPENARLILTVGSEGRVRGINITQSIPGEMPRIIAAVQNWRFRPA
                     TENGRPVTASFAVDITVRPR"
     gene            3530..4132
                     /locus_tag="VNN25_23645"
     CDS             3530..4132
                     /locus_tag="VNN25_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94591.1"
                     /translation="MTADPQTAASDRVTCAACRREIDSVARICPYCGADPRTGEKPVD
                     TQAMLNEVFHPRETSASETVVQFARQRQGVVVALGVVLVILLLAGLHEYVTRRNARDV
                     SAAAAIPLTEVTDLTNQQDENKPLPMPDLKYAFDGHPQTMRTFVTEPGAVVPPEVVAA
                     QQAAAQQAAAQKAGQPLAATPLAAPKPLPAQPLRPTSNHR"
     gene            4158..4418
                     /locus_tag="VNN25_23650"
     CDS             4158..4418
                     /locus_tag="VNN25_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94592.1"
                     /translation="MSAAWWADGGGTPPGQPPRRQRSIRKVANARQMTNLPQLASGTL
                     ASRRLARRRLACGLCKSRDLAEPIDPRRHPFLHLILIGFDQE"
     gene            4450..>4802
                     /locus_tag="VNN25_23655"
     CDS             4450..>4802
                     /locus_tag="VNN25_23655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94593.1"
                     /translation="MLQIIIIVAAVILIGWLAAWMLAQKPVTNAQQLIQQHRYADAIT
                     AAKDDPLHRAEALKLLGRFDDAIASYRQSDDPAAREGIALSLAHLGRDLDEAKRLMEE
                     TIALHPQIMEFQALGL"
BASE COUNT          831 a         1641 c         1468 g          862 t
ORIGIN      
        1 gatcgtccta cgccgcgatg cctgccggtg caaatgcagc gtggggcgcg gctccggcga
       61 ctcaggatga caccacacgg tttgcgcgtg attagggttg agtgtcatcc ggagttgatc
      121 gtccaatcga gattggtgtc atcctgagac tgccggagcc gcccttcgta ctcgatccct
      181 cgcgacgcgt ggagtggcgg cgtaggacgt cgaaggatct caagtttcgc agatcgtgct
      241 cgtctcgccc gagcagtggt gatcattgac gttcgctcgc gctgggcaaa cacgatccgt
      301 aaagtttgag atccttcgat cgtcctacgc cgcgatgcct gccggtgcaa atgcagcgtg
      361 gggcgcggct ccggcgactc aggatgacac cacacggttt gcgcgtgatt agggttgagt
      421 gtcatccgga gttgatcgtc caatcgcgat tggtgtcatc ctgagactgc cggagccgcc
      481 cttcgtactc gatccctcgc gacgcgtgga gcggcggcgt aggacgtcga aggatctcaa
      541 gtttcgcaga tcgtgttcgt ctcgcgcgag agtttgcgac cattgacgtt cgctcgcgct
      601 gggccaacga gatcttcgta gtttgagatc cttcgatcgt cctacgccgc aaagcctgcc
      661 ggtgcaaatg cagcgtgggg cgcggctccg gcgactcagg atgacacgag ggatgatgcg
      721 cggatggtgc aaggggtgat acaagcaagc tgatttcgcg ctgccaaccc agcgtgaaat
      781 ccgcacgcct atggcctctg ccagcgtcgc gcgcgcccat gatcgcgctg ctgcacgccg
      841 cgccccagct cgccgaacat gccggggaac gtcgtgcgca tgcgctcgag atcgggatcg
      901 ttcggccagc gttgctcgat ctcgtcgctg agccgctcaa cctcccgctt ctcgtgcgtt
      961 gcgagggcca tcccgaggtg cgtgagctgc tgctcgcgag ggccgagacg gtccgtgtcg
     1021 tcgagtgctt tctgaagttc ctggctcgcg tagtcgaggt ttcgattgag gagggcgatc
     1081 tctcccaggc ggaagtgcgc cttcgcgtag tgagggtcct gctgcaggac acgatgcagc
     1141 gtcttccgcg cctccatcgc gtcaccgtcg cgaacctgcc tcatggcatt cgtgtagagc
     1201 gcgtccgctc ccggcgctga ctccgattca gccggtgccg atgccgttgg ctcagcggta
     1261 gtctgcgtct cttccgcggt atcggtcgtc ggcgtcgtcg atgtcttccc gacctgtttc
     1321 gccgtcgtag tcgtattcga cgtatcgctc gcagtctctt cgaccgtcgc gtcggaaggc
     1381 gcggtcacat tcacagcggt cgacgactgc gcagcggaag tctgcggcgg cacgaccgat
     1441 tgcgatgccg tcaccttcgg ctccgatcga tgcgttgccc acacgcccgc taccgcagcg
     1501 aggatgatca ccatcaccac gatcaccgcg gctaacgcac cgcggccctt tggcttcgcc
     1561 ggaggttgcg gagcggccag ctcctgcaca gtcggggctt gcggtccggt gcgaatcgtc
     1621 ggagccaccc cttgaatctg cgtgggagct gccgtctgca cttgcggtcc ggtgcgaact
     1681 gtcggatctc ctcctgcagc cgcggccacc accaccgtcg gcgccgccga ttgcggcgac
     1741 gtgtacggca ccgtgccggc gcggccgagt tgcgtgtcga gcgcggtcac cagctccgcc
     1801 gccgtgtatc gctcgcccgg attcttgcgc agcgaccggt cgatgatcgc cttcaacccc
     1861 ggcgagactc ccgggttgac ggcagcggca tccaccggct cgtcgcggat gatctgcgtc
     1921 atcgtctccg tgatcgtctc gccttcgaac ggcttgcgcc ccgtggccat ctcgtacaac
     1981 acgacgccga gggagaagac atcggtacgg ccgtcgaagg tcttgcccag agcttgctcc
     2041 ggcgacatgt agtgaacggt gccgaggatc atgccctgct gcgtgacgaa ggtttcgggc
     2101 gatgctcccg gcgcgccaac ctgcttggcg atcccgaagt cgagcacctt cacgcgattt
     2161 ccggccacga tgatgttgtc aggcttgatg tccctgtgca cgatgccttt tgcatgtgcc
     2221 tcggcaagag catcagccac cgcgcgggcg atgcggcaga tctccgtttc ggccagaccg
     2281 ccgcgcttga tcactttcgt cagtgaatca ccttcgacga actcttcaac gatgaagatg
     2341 tggtcgccat cctgctcgat cgagtggatc gtcgcgatcg atggatggta gagctgggct
     2401 gcggtgcgcg cttcccgttt cagccgcgac gtcgcctcgg catcggcaac gaagctcggc
     2461 ggcagcacct tgattgccac caccctgccg agccgcgagt cctcggcgcg ccagacttcg
     2521 cccatcccgc cggcgcctac gcggttgatg agacggtacg gtccgagcaa ctggtcgggg
     2581 gaaagcacct gcgcattgta ccggtacggc ggttgcagca gagctgcgaa gaagaaagag
     2641 ttatgtctga acgaaaaccg tgtgtccgtt gtgagcgtcc gatcgattcc tctgcccgct
     2701 cgtgtgttta ttgcggttgg gatcaggccc ggccccgccc cgccgaatcg acgacgccac
     2761 cgatcgccaa cccgtacacg ccgcctgccg ataaccgggt ccgcaaccgg ctcctcggcg
     2821 cgggggcatt cgtcgtgctc gtgatcctgg cgttcttcgt cggatcgctg ctcaaccgct
     2881 ccgaaggcaa tgagacaagc gcctccgaga aggcgtcgaa tccatcgccg cagaccggcg
     2941 ccggatcgtc gtcgcgcacg accgtcatgc tcgtgccggt cgatggaacg agcccgttgc
     3001 aggaacagcc accggtcacg agtgcgcccg caccaccagc gagcgcgggc agcgcggaga
     3061 tcaacggcga gcgttccgac gccaccgcgc tgccgtccgg cgcatactcc gtcgcagtcg
     3121 agcgcgcccg ggcggagcgg gagaaacacc caaacgcagg catcgtcgat cctcgcacga
     3181 ttgcaggcgg cgttgaatca ggaccggctc gccgcaccgc atcgagggaa gcacaacgcg
     3241 cgaatgcaca gcgcgccgca aacaacatcc cgacggagtc gctgcgcacg cagccggttc
     3301 ctctctatca gccagttccg tcgatgcagg tctatcgtcc ggagaatgcg cggctgatcc
     3361 tgaccgtcgg ctccgaaggg cgcgtacgcg gcatcaacat cacgcagtcg atcccgggcg
     3421 agatgccgcg gatcatcgcc gcggtgcaga attggcgttt ccgtccggca accgaaaacg
     3481 gccgacccgt gacagcgagc ttcgctgtcg atataacggt gcggccgcga tgactgccga
     3541 tccgcaaacc gccgcctctg accgcgtcac ctgtgccgcc tgccggcgcg agatcgattc
     3601 tgtcgctcgg atctgtccct attgcggtgc cgatccacgc accggcgaga agccggtcga
     3661 cacgcaggcg atgctcaacg aggtcttcca tccccgcgag acctcggcat ccgaaaccgt
     3721 cgtgcagttc gcccggcagc gacaaggcgt ggtcgtcgcc ctcggcgtcg ttctcgtcat
     3781 cctcctcctg gccggcctcc acgaatacgt gacgagacgc aacgcccgcg acgtctccgc
     3841 cgccgccgcc atcccgctga cggaagtcac cgacctcacc aaccagcagg acgagaacaa
     3901 acctctgcca atgcccgacc tcaaatacgc cttcgacggc cacccgcaaa cgatgcgaac
     3961 gttcgtcacc gagccgggcg cggtagtgcc ccccgaggtc gtcgccgcac agcaagccgc
     4021 cgcgcaacag gcggcagcac aaaaggccgg acaaccgctc gcagcaacac cgctggccgc
     4081 gcccaaaccg ttgccagcac aaccactccg tccgacttcc aatcatcgct aatctccaat
     4141 cgagcgcgga aggccggatg agcgcggcgt ggtgggctga cggcggcggg acgccgccgg
     4201 gccagccgcc gagacggcag cgttccatcc gcaaagtggc caacgcgcgc caaatgacta
     4261 acctgccgca gctggccagc ggaacgctgg cgtcccgccg gctggctcgt aggcgtctcg
     4321 cctgcggtct gtgcaaatca cgcgatctgg ccgaaccgat cgatccgcgt cgccatccgt
     4381 ttctccatct aatcctgatc gggttcgatc aagaatgagg gcccacgatc aaagtagact
     4441 ctcaacacca tgcttcaaat catcatcatc gtcgccgccg tgatcctcat cggctggctc
     4501 gccgcctgga tgctcgcgca aaagccggtc accaacgccc agcaactcat ccagcaacac
     4561 cgctacgcag atgccatcac cgctgcgaag gacgatcctc tccaccgcgc cgaagcgctc
     4621 aaactcctcg ggcgcttcga cgatgcgatc gcgtcgtacc gtcagtcaga cgaccccgcc
     4681 gcccgcgaag gcatcgccct ctccctcgct cacctcgggc gcgacctcga cgaagcgaaa
     4741 cgcctgatgg aagagacgat cgccctccac cctcagatca tggagttcca ggccctcggc
     4801 ct
//
LOCUS       DATLVW010000806         5589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_77980, whole genome shotgun sequence.
ACCESSION   DATLVW010000806 DATLVW010000000
VERSION     DATLVW010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77980"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VNN25_23660"
     CDS             <1..580
                     /locus_tag="VNN25_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94594.1"
                     /translation="RSRRAIDRTFHLINSSLQVIEASERFAAEGPVRASRQLRRVGFW
                     LSDAAGKLDRARAGLRDVLDRAEQFPKVALDAPEKVIEASLCWVVAYEQLAAISSRFE
                     DTLTWLADSVESGAIAIPIEEQAADAGKAVIVLRRSGAPCIPPDLLARESNRIPFRRQ
                     RSICLTVAEAVRRIVRGRAPPSSQTARTDHSS"
     gene            599..1174
                     /locus_tag="VNN25_23665"
     CDS             599..1174
                     /locus_tag="VNN25_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94595.1"
                     /translation="MSSSNEVPVTPPQESYADSALALVEELRAMRERIPNLVIPETKD
                     ARRRLSQAASVPPEFVELTSMAVKSHAPLVRGGGQDLARNADLKSFAEAYGPVGDELE
                     ALTSFVKHSVTKAMNTVGSEALTTFALAKRLSKRPESADLIPHVDAMGKALGKRGRKS
                     KSKPAPKPAAPVSTESSPVTSSSSTTDPKSK"
     gene            complement(1589..2380)
                     /locus_tag="VNN25_23670"
     CDS             complement(1589..2380)
                     /locus_tag="VNN25_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94596.1"
                     /translation="MPSHAQREFSRLGPHAFELGNLAYIRAVVLTYMDRLGEATTQAV
                     ESARIFAAYGDDERWMRARSVEAGVLFYRREFAEAADAFEQLRAYAESKGDAIEMARQ
                     SYNMATCFLELGDAARAALLLLDARRVYQEQDVRTGLVRTDWKLGVQSRVAGRFEESV
                     AQLRAARKASEDLRLPEEAAHITLDLIESLLLAGTAREISVLCSEVMRYFRRSGKLRQ
                     ALTAAAFLKEAAAQGTIRVETVQHVRSFVQRLERLPDLLFAPPVD"
     gene            2454..2576
                     /locus_tag="VNN25_23675"
     CDS             2454..2576
                     /locus_tag="VNN25_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94597.1"
                     /translation="MNCIEWVVVGRELFRDDDCGVGQIPGVQRVALEGIVGGLG"
     gene            complement(2930..3523)
                     /locus_tag="VNN25_23680"
     CDS             complement(2930..3523)
                     /locus_tag="VNN25_23680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH94598.1"
                     /translation="MPAELTPLDEASVPSPPLACIESPPPPLSGQEAFAEIYESHARL
                     LRLIAVRDYRVPFGDAEAIVHDIFMHYFSNPGVVRGELKPYFRGAVRNECLEYWRKRQ
                     REQTVFCGVDDVEHASSDEDALMSDTIAVRLAVAETMARLRPRCRDALRCFHYEGKSM
                     RDVAAELDVAPLYVRQLLHHCRKAARAIFESITRVAS"
     gene            complement(3647..3874)
                     /locus_tag="VNN25_23685"
     CDS             complement(3647..3874)
                     /locus_tag="VNN25_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94599.1"
                     /translation="MRCDRPLQEPASPFIRSDGASLHEAVVDALARILLEVDEALTLL
                     DGEEANRFLWIEPHLRNVFDEINSVMFRLTD"
     gene            4262..4696
                     /locus_tag="VNN25_23690"
     CDS             4262..4696
                     /locus_tag="VNN25_23690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94600.1"
                     /translation="MSVAEIVVDHHLYIPANDWLPSVLIVEDERKLRALLVEEFRALH
                     IDALTASSGYEAIRIASEHRPDLILVDGLLPEMHGFEISRFIRRIDVDYHPHIALMTA
                     IYKHTRYQNEARLKYGIDDYLIKPVHPQMLAGLIRRVQAATR"
     gene            4693..5157
                     /locus_tag="VNN25_23695"
     CDS             4693..5157
                     /locus_tag="VNN25_23695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94601.1"
                     /translation="MSTLNPPRGRVLIVDDQIEILAGMKALLEAEGLDVTTHDSMITL
                     PLVLRDANPDLILLDLSFGALSGESFFRLGRERLLRKTDAMVVLFSGLPLAQLAAKAE
                     EFGADGVIPKSDDTMQIVARVKTLIKQHQYLRIARERTAFSDPTRTASAPAS"
     gene            5120..5491
                     /locus_tag="VNN25_23700"
     CDS             5120..5491
                     /locus_tag="VNN25_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94602.1"
                     /translation="MTQLVPHLLLRADPGSSATASLLRSAGYMVSRIDDDTIAVEIAG
                     SNHVDGLIVELPALAAITFARRIEARHDQKVAMLVVTPSVDALRRAMPHVHTIRTSQV
                     GDDLISTIDLALVAHQMQRAG"
BASE COUNT         1092 a         1753 c         1725 g         1019 t
ORIGIN      
        1 ccgctcgcgg cgggcgatcg atcgcacgtt ccatctgatc aactcctccc tgcaagtcat
       61 cgaggcctcg gaacggttcg cggcagaggg gccggtccgg gcatcgcgtc agctgcgacg
      121 cgttgggttc tggctgtccg atgccgccgg gaaattggac cgcgccagag ccggtctgcg
      181 cgatgtgttg gaccgcgcgg agcaattccc caaagttgcc ctcgacgccc cggagaaggt
      241 aatcgaggcg tccctctgct gggtcgtcgc ctacgaacag ttggccgcga tctccagccg
      301 gtttgaagac accctcacct ggctggccga ttccgtggag agcggtgcca tcgccatccc
      361 gatcgaagag caagcggcgg atgcagggaa ggcggtcatc gttcttcgga ggagcggggc
      421 gccatgcatt ccacccgacc tgctcgcccg tgaaagcaac cgcattcctt tccggcgtca
      481 acggtcgatt tgtctgaccg tggccgaggc cgtaagacgc atcgttcgag gacgtgctcc
      541 gccttcatcg caaactgccc gcactgatca cagcagttga gttgactatg gagggactat
      601 gtcttcatcc aatgaagtac ccgttacacc cccacaggag tcgtacgccg attccgcgct
      661 ggcgctcgtc gaagagcttc gggccatgag ggagaggatc ccgaatctgg taatcccgga
      721 gaccaaagac gcacgccggc ggctgtcgca agcggcttcg gttccgccgg agtttgtcga
      781 gctgacgtcg atggcggtca agagccacgc gccactggtc cgaggaggtg gccaggatct
      841 cgcgcggaat gccgatctga agagctttgc cgaggcgtac ggtccggtgg gtgacgagct
      901 ggaagcgctg acttccttcg tcaagcacag tgtcaccaag gcgatgaaca cggttggaag
      961 cgaagcgctg acaaccttcg ccctggccaa gcgtctgtcc aaacggcccg agtcggccga
     1021 tctcattccg cacgtcgatg cgatgggaaa agccctcggc aagcgcggac ggaagtccaa
     1081 gtccaagccg gccccgaaac cggcagcccc ggtttccacg gagtcctcgc cggtgacatc
     1141 ctcgtcatcg acgaccgatc caaagagcaa gtaaccgctc tcgtgcagta agtcccgcgc
     1201 gggcgcgtcc gaaggcgcgc ccgcgcattt gcatcgtaga gcagacattc ctgtctgctc
     1261 ccgtcgggcg tccccgcccg acggtggttt ctctgcgcaa atcgtcacgt tgcgatcaac
     1321 cgggggggaa actgatatcg atcgtgtcga acgcccgaat gatcatccgg acaagggaaa
     1381 cgaagccacc gcctctttcg gacgttttcg cttcggcgaa agccggtggg acgactagcg
     1441 ttgcgaccac tcccgcaacc gcgagcattt tgacaatgcg tctggataca cctctcatgt
     1501 gccctcctga gccgaacttg ccggcaaagt ctgagagggc gccctcgtaa acacagaggg
     1561 aatgagttgt gtgatgtttg ggaaggaact aatcgaccgg cggagcgaag aggagatcgg
     1621 gcaggcgctc cagccgttgc acgaacgaac ggacgtgctg caccgtctcg acccgaatgg
     1681 tgccttgagc cgctgcctcc ttgaggaagg ccgctgcggt caacgcctgc cgcagtttcc
     1741 cggagcgtcg gaagtagcgc attacctcgg agcaaagaac ggagatctcg cgcgctgtgc
     1801 ccgccagcag cagcgactcg atcaaatcga gcgtgatgtg cgcagcttct tccggcaacc
     1861 tcaaatcttc cgaggctttc ctggccgcgc gaagctgtgc gacggactct tcgaagcgtc
     1921 cagccacgcg agattgaaca ccaagcttcc aatccgttcg aacgagacct gttctgacat
     1981 cctgttcctg gtaaaccctg cgtgcgtcca ggagcagcag cgcagcacgt gccgcatcgc
     2041 ccagttcaag gaaacacgtc gccatgttgt aggactgtcg tgccatctca atcgcgtcgc
     2101 ccttcgactc cgcatacgcg cgcaactgtt caaaagcgtc cgccgcttcc gcgaactccc
     2161 gtcggtagaa cagcactccc gcctcgacgc tgcgcgcacg catccagcgc tcgtcgtcgc
     2221 catacgcggc aaaaatcctt gcactttcga ccgcttgcgt tgtcgcttcg ccgaggcggt
     2281 ccatgtaggt gagcacgacg gcccggatgt acgcgaggtt gcccagctcg aaggcgtgcg
     2341 ggccgaggcg ggagaattcg cgctgggcgt gagagggcat cgagcgcggc cgggtactcc
     2401 gccaggaacc gcagcgcgtt ggctcgctcc ttccacgcca gaccgcgcag cgtgtgaact
     2461 gtatcgagtg ggtagtcgtc ggtagagagc tgttccgcga tgacgactgc ggcgtcggcc
     2521 agattccggg cgtgcaacgg gttgcgctcg aaggcatcgt tggcggcctc ggatagcact
     2581 cgtaccgtcc cgccggtccg gtagccctcg tcacgctcga tgcgctcccg aatgaagccg
     2641 accgtgtccg cgagcagcgg agcgagcgct cgggccgcct cggcgtactc ctgttgcgag
     2701 cgcgccgcaa actccagaat ggcgcgctgg ttcgcggcaa cagcggcgcc tgcatcggcg
     2761 gcttcccaca tgtccggcga cgtcagggcg tcgaagaatg cggcgatgcg cccgtagcgt
     2821 gcctggcact cctcgcactc attcaagtga ccggcgatcg ccgcagacgc cgaacggttg
     2881 cgtccatcgg caactgcata ctccgtaagc gcatcttcgt cgatatgggt catgacgcca
     2941 cccgcgtgat ggactcgaag atggcacggg ccgccttacg gcagtgatga aggagctggc
     3001 ggacgtagag cggcgcgacg tcgagctccg cggccacatc gcgcatcgac ttcccttcgt
     3061 agtgaaagca gcgcagggcg tcgcggcacc gcggccgcaa gcgtgccatc gtctcggcca
     3121 cggccagacg cacggcaatc gtgtccgaca tcagcgcgtc ttcgtccgag gaggcgtgct
     3181 cgacgtcgtc gacaccgcaa aagaccgtct gctcgcgctg ccgtttccgc caatactcga
     3241 gacactcatt cctcaccgcc cctcggaagt acggcttcaa ctcaccgcgc acgacgcccg
     3301 ggttgctgaa gtagtgcatg aagatgtcat gcacgatcgc ttcagcatcg ccaaacggaa
     3361 cgcgatagtc gcgcacggcg atgaggcgca gcaatcgggc gtgactctcg tagatctcgg
     3421 cgaaggcttc ctgtcccgac aagggaggag ggggtgactc gatgcaggcc aacggcggtg
     3481 acggcacact tgcttcgtca agcggagtca attcggcagg catgggaggc gcggtcagta
     3541 tggcgcgcct tacgtaggta acgcaagaac gggtgccggg agtcgggagt cgggggtcgg
     3601 agatccgaag ccgttgacga tacgcgctaa acgcgcggtg tcatccttaa tccgtcaacc
     3661 ggaacatgac ggaattgatc tcgtcgaata cgttgcgcag gtgcggttcg atccagagga
     3721 agcggtttgc ttcctctccg tcgaggagcg tcagcgcttc gtccacttcc agaaggatgc
     3781 gggccagtgc gtcgacgacc gcttcatgaa gcgaggcccc atcgctccga atgaacggtg
     3841 aggcgggctc ctgcagtggg cgatcgcagc gcatgggttg attgatggaa cgacgccaca
     3901 atacgcaggt agcctgacgg ctactagtca cgttttttga cctttcgaac gcttgctgat
     3961 cgggggtcgc gtttcgggag tcgggagtcg gatcagaggc ggggatgatg ccgcgctccc
     4021 acacccgact accgactagc gactccccat cgtttggaga aggcccagtc ggccggcgca
     4081 cggacattcc gttaaccacc ccgtcccatt tccggacact cctccgaaaa tcgcacggcg
     4141 ctgaacgaag caagaatgcc ttgttcctct acgcctgcaa caatttagct cggtgcgtgg
     4201 aaatggcagc gaccttggac cactactact ccgagttcac gagcgaaaga gggtccaaac
     4261 catgagtgtc gcggaaatcg tagttgatca ccatctctat atcccggcga atgactggct
     4321 gccgtctgtg ctcattgtcg aggatgagcg gaagctgcgg gccttgctgg tggaggagtt
     4381 ccgggccttg cacatcgatg ccctgacggc cagcagcggc tacgaggcca tccggattgc
     4441 cagcgaacac cggccggatc tcatcctcgt cgacggattg ctgccggaga tgcacggatt
     4501 cgagatctcc cgtttcattc gcaggatcga cgttgactat cacccgcaca tcgctctgat
     4561 gacggccatc tacaagcaca cgcgctatca gaacgaagcc aggctgaaat acgggatcga
     4621 cgattacctc atcaagccgg tgcatccaca aatgctggct ggactgattc gccgcgtaca
     4681 ggcggccacg cgatgagcac cctcaatccg ccgcgcggcc gcgtgctcat cgtcgatgac
     4741 cagatcgaaa tcctggctgg gatgaaagcg ctcctcgaag ccgaaggtct tgatgtcacc
     4801 acacacgact cgatgatcac gctgccgttg gttctccgcg acgccaatcc cgacctgatt
     4861 ctcctcgatc tcagttttgg ggctctgtcc ggtgaatcgt ttttccggct gggacgggaa
     4921 cgcctgcttc ggaaaacgga cgccatggtc gttctttttt cgggcctgcc cctagcgcag
     4981 ttggcggcaa aggccgagga gttcggcgcc gatggcgtga tcccgaagtc ggacgacacg
     5041 atgcagatcg tggctcgcgt caagacgttg atcaaacaac atcaatacct gcgcatcgcg
     5101 cgggaaagga cggcattcag tgacccaact cgtaccgcat ctgctcctgc gagctgatcc
     5161 cggctcatcg gcaaccgcgt cgctgctccg ctccgccggc tacatggtga gcaggatcga
     5221 cgatgacacg atcgcggtgg agatcgccgg cagcaaccac gtcgacggct tgatcgtcga
     5281 actgccggcg ctcgcggcaa tcactttcgc gcgccggatc gaagcgcggc acgatcaaaa
     5341 ggtagccatg ctcgtcgtca cgccgtctgt cgatgcactc cgccgcgcga tgccgcacgt
     5401 gcacacgatc aggacttccc aggtcgggga cgatctgatc tcgacgatcg atctggcgct
     5461 ggtcgcacac cagatgcaaa gggccggata agaaaatgcc cgttgctcgg ccggcgcaac
     5521 gggctggcgc gctagagtgc gcccactggg tcgctgcaag aattcggatc agcgacagaa
     5581 ccgaactta
//
LOCUS       DATLVW010000807         5273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_32944, whole genome shotgun sequence.
ACCESSION   DATLVW010000807 DATLVW010000000
VERSION     DATLVW010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32944"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..945
                     /locus_tag="VNN25_23705"
     CDS             253..945
                     /locus_tag="VNN25_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXH94603.1"
                     /translation="MTIAGVILAALAFFSLLLSGPGTRWGWWDFKVGLALFAVAFLVG
                     ALALLVSGFAWWRTRSNPVPAIAAIACAVIVLLPLTQIVGARGKPRIHDISTDLADPP
                     HFAAVLPLRKGANPAPDRIDVKTAGLQRAGYPDLVTLDLPLNSDAAFAKILQAAKTME
                     WEIVSVRPAEGILEATATTAWFGFHDDIVVRVRAAGEQSRVDVRSTSRVGVGDAGKNA
                     DRIRKFLALLRS"
     gene            1027..3276
                     /locus_tag="VNN25_23710"
     CDS             1027..3276
                     /locus_tag="VNN25_23710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH94604.1"
                     /translation="MRTAVFSIISPNYRHFVRVLMDSVRRHHPEWERFLLIVGGESQA
                     GSREPDESLTTMSIDALPLPNPRQFYFRYTIFELDTAMKPWMFEHLFGLGYDRVVFLD
                     PDTCVYSPLVELDALPPETFLAVTPHLTGSISGDDHPSERSILLAGTYNLGFLAVWRQ
                     PALARFLDWWKEKLEFQCVVDTARGLFADQKWIDLAPGLFPGVAIIRHDGYNVAYWNL
                     EQRAVAGEGSEVTINGQPLRFYHFSGLDPAAPSMLSKHEHGRKLSDIGDARKLVDDYL
                     VLLRAAGFEPSRASPYAFGTFHDGKPVPDAARIAYRNSIELQDAAGADPFAHPELFAE
                     YSGPAPRPALLARAGGVSYQWLSRLRPLVRLFPRHVRKAMRETLLGRRDDSPGPVRHQ
                     STLPAGLNIIGYIGRETGVGESARLCQKSCDAIGLANHLVDVDGDEGTQQAVYGASIF
                     HLNADQAPGVHEQLRSGFEANAYNIGCWHWELPELPDAWIASAAPLDEIWAPSAFIQN
                     AISRKVRIPVVHMPHGIEVSNIEICTPGELGVPAGRFTFLCMFDLANVTARKNPLGAI
                     DAFQRAFSSSSPVALLIKVSQAADHPTEYAAIEECIRGISNMYLTDRMLSRGRVNGLI
                     AACDAVVSLHRSEGFGLTFAEAMFLGKPVVATGWSGNMDFMNGRNSCPVDYELVKLDR
                     AHGPYAAGQQWAEPDVDHAAQFMRRLLDDAAYRARIGERARDTMRTQFSLAGAGLRYQ
                     QRLRFLGLL"
     gene            3422..3498
                     /locus_tag="VNN25_23715"
     tRNA            3422..3498
                     /locus_tag="VNN25_23715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3456..3458,aa:Arg,seq:tcg)
     gene            3537..5192
                     /locus_tag="VNN25_23720"
     CDS             3537..5192
                     /locus_tag="VNN25_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94605.1"
                     /translation="MTVDAASRSVIRTRLAIFVAAALLVAIRITLPANAFWISDAGNK
                     NLVAENFAASGYRHVSIGYPAADIDPGFRWFPAAGSHFRQLGTHYFSIVPWVFPLLAA
                     PLGTPWIPLAAGIGILMMMPALLRAAGSDADPAWAVIVTAFATPLAFYSLDFWEHTLA
                     TLLATGAVVLLLRRKPLLAGLAAGASIVIRDEGYVFLAAVIIALAVARELRPRIAPLL
                     LGAAIVTAPYWFAQWMIFGNPFGLHLAVHAAADDVPLPARIVRNVLYFLFHFHGRPLI
                     AALLAVPAAAAIVIGFRRRAPEQESRAEVIAAVLVGIGSLIGIIFWITDSDPIVNTIY
                     TQGLFLFLPFTVLFLTRWRALVTGDDAMALIARIAAVYVVLMPLVLRVNWSGVVWGPR
                     YFLTIVPLLVMLSLRRSKIAVALVVISFVIEGYGLVLLHRKLEATSQLVTIVRTAPQV
                     VISDVYWLPEELSALYFEKKFMQPFGDRELFVALATLRAGNVREVTVVLSPRYHWYSP
                     NARALLESMAVRRVTFDPPGVPLLRAEVLVCQLSPRSENLQPR"
BASE COUNT          851 a         1674 c         1636 g         1112 t
ORIGIN      
        1 gccgccagaa gaaacgggac acgatggttc atcgcgtgat cctcatctga gttgtttccg
       61 gggttgtagg tttcgggttg accgtcaatg tacgtccggt ttggtgccgg cgcacgaaaa
      121 agacaccttt atgagtcgcc caaagcggtt cttttcggaa acgtgacgga ggatctggcg
      181 gaaagggatc gtttatggaa ccactgagcg tccggcagtt ccggaccgtt atcctcgaac
      241 tacacttaca cgatgacgat tgccggtgtg atcctcgcgg cgcttgcctt cttctcgttg
      301 cttctctccg gtcccggcac gcgctggggg tggtgggatt tcaaggttgg tctggcgctc
      361 tttgcggttg cgtttctcgt gggagcactt gcgcttctcg tctcgggatt tgcgtggtgg
      421 cggacgcgct cgaatccggt gcccgcgatc gctgcgatcg cgtgcgcggt gatcgtgttg
      481 ctgccgctca cgcagatcgt gggcgcccgc ggtaaaccac gcattcacga catcagcacc
      541 gaccttgccg acccgccgca cttcgccgcg gttcttccgt tgcgcaaagg tgcgaacccg
      601 gcgccggacc ggatcgacgt caagacggca ggtctgcagc gggcgggcta tcccgatcta
      661 gttacgctcg acctgccgct caacagcgac gccgcttttg cgaaaatcct gcaggcggcg
      721 aagacgatgg aatgggagat cgtctccgtg cgtccggcgg aaggcatcct cgaggcgacg
      781 gcgacgacgg cgtggttcgg attccacgac gacatcgtgg tacgcgttcg tgccgcggga
      841 gagcagagcc gcgtcgatgt gcgctcgacg tcgcgcgtcg gcgtcggcga tgccggcaaa
      901 aacgccgatc ggatccggaa gttcctggcg ctccttcggt cgtgagcgtt ctccgcgtga
      961 aacaagaacc tgttcacgcg gaggcgcgga gaacccggat cggatacatt cgcgcgtccc
     1021 agtccgatgc gcaccgcggt cttctcgatc atttctccga actaccggca cttcgtgaga
     1081 gtcctcatgg actctgtcag gcgacatcat ccggagtggg agcgattctt actcatcgtg
     1141 ggcggggagt cacaggccgg gagtcgggag ccggacgaga gcctcacaac gatgtcgatc
     1201 gatgccttgc cgctgccgaa tccgcgtcag ttctacttcc gctacaccat tttcgagctc
     1261 gacaccgcca tgaagccgtg gatgttcgag cacctctttg gacttggcta cgaccgcgtc
     1321 gtctttctcg atccggacac ttgcgtttac agcccgctcg tcgagctcga tgcgctccct
     1381 ccggaaacgt tcctggcggt gacgccgcat ctgaccggat cgatctccgg cgacgatcat
     1441 ccgagcgagc gctccatcct cctcgccggc acctacaacc tcggcttcct ggccgtgtgg
     1501 cggcaaccgg ctcttgcacg gtttctcgac tggtggaagg agaagctcga gtttcaatgc
     1561 gtcgtcgata ccgcgcgcgg actcttcgct gatcagaagt ggatcgatct cgcgcccggt
     1621 ctctttcccg gcgtcgcaat cattcgtcac gacggataca acgtcgctta ctggaacctc
     1681 gagcagcgag ccgtcgctgg cgagggcagc gaggtaacga tcaacggaca gccgctccgc
     1741 ttctatcatt tcagcggact cgatccggcc gctccatcca tgttgtcgaa gcacgagcat
     1801 gggcggaagc tcagcgatat cggcgacgcc cgcaaactcg tcgacgatta ccttgtcctc
     1861 ttgcgtgcgg cgggcttcga gccgtcccgt gcatcgccct acgcgttcgg gacgtttcac
     1921 gacgggaaac cggtgccgga cgccgccaga attgcttatc gcaattcgat cgagttgcag
     1981 gacgccgccg gggcagaccc gttcgcccat cccgagctct ttgctgagta cagcggcccg
     2041 gcgcccagac cggcgctgct cgcaagagcg ggtggggtgt cgtaccaatg gctctcgcgc
     2101 ctgcggccac tggtgcgcct gtttccgcgt catgttcgaa aggccatgcg cgagactctg
     2161 ttgggacggc gcgacgattc gcccggcccg gttcgtcatc agtcgactct tccggcggga
     2221 ctcaacatca ttggttacat cgggcgggag accggcgtcg gtgagtcggc acgcctctgc
     2281 cagaaatcgt gcgatgcgat cgggctcgcc aatcatctcg tcgatgtcga cggcgatgaa
     2341 ggcacccagc aggctgttta tggcgcgagc atctttcacc tcaacgccga tcaggctccg
     2401 ggcgtgcacg agcagcttcg aagcgggttc gaggcgaacg cgtacaacat cggatgctgg
     2461 cactgggaat tgcccgagct acccgacgca tggatcgcgt cggcggcgcc gctcgacgag
     2521 atctgggcgc ccagcgcgtt cattcaaaat gccatcagcc ggaaggtgag gattccggtc
     2581 gtgcatatgc cgcatggaat cgaagtgagc aacatcgaga tatgcacccc cggggagctt
     2641 ggtgtgccgg ctgggcgctt cacgtttctc tgcatgttcg acctcgccaa cgtcacggcg
     2701 cgaaagaatc cgctcggagc gatcgatgcg tttcagcgag cattttcgag ctcgtcaccg
     2761 gtcgctttgc tgatcaaagt cagtcaggcg gctgatcatc caacggagta cgccgcgatc
     2821 gaggagtgca tccgcggcat atctaatatg tatctgaccg atcggatgct ttcgcgcgga
     2881 cgcgtgaatg gcctgatcgc cgcgtgcgat gcggtcgttt ctctgcaccg ttccgagggg
     2941 tttggactca ccttcgccga ggccatgttc ctcggcaagc cggtcgtggc gacgggatgg
     3001 tcgggaaaca tggacttcat gaacggccgc aattcgtgtc ccgtcgacta cgagctggtc
     3061 aagctcgacc gcgcgcatgg cccgtatgcc gctggccagc agtgggctga accggatgtc
     3121 gatcacgccg cgcaattcat gcggcgactg ttggacgatg ccgcgtaccg cgcgcggatc
     3181 ggcgaacgcg cccgcgacac gatgcgaacg cagttcagtc tcgcaggggc gggacttcgc
     3241 taccaacaaa ggctgcgctt cctcgggctg ctctaaggag tgcaggcgtc tcgcccggcg
     3301 gtaaacctcg gcgatgcgaa caaaacgtcc ggcgttacga aaaccacgcc gaggcgggga
     3361 cgcctcgccg ccgcgagcga gacgctcgca ctcctcgcaa ctgtggtagt ttttctctga
     3421 cgcgccctta gctcaactgg acagagcatc agacttcgga tctgagggtt gggggttcga
     3481 atccctcagg gcgcaccaag ctcctccggc tgcgaagatt gtggatctcg ccagcgatga
     3541 ccgtcgacgc agccagccgc agcgtgatcc gaacacgtct cgccatcttc gtggcggcag
     3601 cgctgctcgt ggcgatccgc atcacgctcc cggccaacgc gttctggatc agcgacgccg
     3661 gaaacaagaa tctcgtcgcc gagaacttcg cggccagcgg ttatcgccat gtctcgatcg
     3721 gctatccggc cgcggacatc gatccgggtt tccgctggtt ccccgccgcc ggtagccatt
     3781 tccgccagct cgggacgcat tacttctcca tcgttccgtg ggtgttcccg ctgctcgccg
     3841 cgccgctggg gacaccctgg attccactcg cagcaggcat cgggattctg atgatgatgc
     3901 cggcgctact gcgtgcggcg ggtagcgatg ccgatccagc ctgggcggtg atcgtgaccg
     3961 cgttcgcgac tccgctggct ttctattcgc tcgatttctg ggagcacacg ctggccacgc
     4021 tgctggccac aggcgcggtc gtgttgctcc tgcggcggaa gccgttgctg gccgggctcg
     4081 cggcaggcgc ttcgatcgtc atccgcgatg aaggctacgt gtttcttgcc gcggtgatca
     4141 tcgcactcgc cgttgcgcgg gaactgcgcc cgcggatcgc gccactgctc ctcggtgccg
     4201 cgatcgtgac tgcgccctac tggttcgcgc aatggatgat cttcggcaac ccgttcggcc
     4261 tgcatctcgc tgtgcatgcg gcggctgacg atgtcccgct gccggcgcgc atcgtccgca
     4321 acgttctgta cttcctcttc cattttcacg ggcggccttt gatcgctgct cttctcgcgg
     4381 tgccggcggc tgcagcgatc gtgatcggat ttcgccgccg cgcgccggag caggagtctc
     4441 gcgcagaagt catcgccgcg gttcttgttg gcatcggctc gctcatcggc atcatcttct
     4501 ggatcactga cagcgatccg atcgtcaaca cgatctacac gcagggactc ttcctctttc
     4561 tgccgttcac ggtcctcttc ctgactcgat ggcgcgcgct tgttaccggt gacgatgcga
     4621 tggcgctcat cgctcggatc gccgcggtct atgtcgtgct catgccgctg gttctgcgcg
     4681 tgaactggag cggcgtcgtc tggggtccgc gctacttcct cacgatcgtt ccgctactgg
     4741 tcatgctctc gctgcgtcgg tcgaagatcg cggttgcgct cgtggtcatt tcgttcgtga
     4801 tcgagggtta cggcctcgtg cttctgcacc gcaaactcga ggcgacgtca cagctcgtga
     4861 ctatcgtccg caccgcgccg caggttgtga tcagcgacgt ctattggctc cccgaggagt
     4921 tgagcgcgct ctatttcgaa aagaagttca tgcagccgtt cggcgatcgc gagcttttcg
     4981 ttgcgcttgc aaccttgcgc gccggcaacg ttcgcgaggt tacggtcgtc ctttcgccgc
     5041 gctatcactg gtactcgccg aatgcccggg cgctgctcga atcgatggcc gttcggcgcg
     5101 tcaccttcga ccctcccggc gttccgttgc tgcgggccga ggttctggtc tgtcagcttt
     5161 cgccccgatc cgagaatctt cagccgcgat gagcaggttg aggcctgacg tggatctcaa
     5221 cgcctgctac ctcgccgcga tgcgcatcct caaccatcgc ttcaacagcg agg
//
LOCUS       DATLVW010000808         4132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_282774, whole genome shotgun sequence.
ACCESSION   DATLVW010000808 DATLVW010000000
VERSION     DATLVW010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282774"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VNN25_23725"
     CDS             <1..303
                     /locus_tag="VNN25_23725"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXH94606.1"
                     /translation="DMKEKGSDVNLATELLVDAFSNAYDAAVIISNDSDLVAPIQAVR
                     KLGKPVGIINPHQRQSLELMKCASFVKQVRTWALAQSLLPSVLTDRNGEFHKPPGW"
     gene            complement(321..734)
                     /locus_tag="VNN25_23730"
     CDS             complement(321..734)
                     /locus_tag="VNN25_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXH94607.1"
                     /translation="MPRIEDFSVGQRASFTKTFTEDDVQRFIAITGDVNPLHVDDAFA
                     AETKFGRRVLHGMLTASIFSTMVGMFLPGTGAIYRSQTLRFLLPVYVGDTATAHFVVR
                     AIDRTKHRLEIEAWIENEAGERVIEGVCEAGLLRG"
     gene            910..2379
                     /locus_tag="VNN25_23735"
     CDS             910..2379
                     /locus_tag="VNN25_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXH94608.1"
                     /translation="MNSEFAPTGIQGLDYILLGGFPRNRVYLIQGDPGVGKTTMGLQF
                     LLEGMRAGEAGLYITLSETAEELRAVATSHGWSLDGLAIYEQMIGEEALAEEETTVFY
                     PAEVELGETIKGMLKEVDRVKPRRIVLDSLSEIRLLAQSGLRYRKQILALKQFFSGRN
                     VTVLCLDDRTSEFQDIQLQSVPHGVVELERYTPVYGAARRRLQLVKVRGLAFRDGFHD
                     FNVITGGIVVYPRLVAAEHRTIATHEQVATGIPELDTMVGGGLDRGTSTLIMGPAGSG
                     KSALATQQVVAAASRGEKAAMFIFEESLGSVLARSASLGMPLEPLVNEGKVTIRQVDP
                     AQLQPGEFAHLVREAVEDRGARVLVIDSLNGFLNAVPEERFLLLHLHELLSYLGQNGV
                     ATILVFAQHGLVGHMQSTVDVSYLADAVILLRYFEAAGRIHKAISVVKKRSGPHETAI
                     RSMTMGKGGLKIGPPLENYRGVMSGIPTYDGTVGPSRDA"
     gene            2376..3968
                     /locus_tag="VNN25_23740"
     CDS             2376..3968
                     /locus_tag="VNN25_23740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXH94609.1"
                     /translation="MTTEAKPKASGHRSDRILIVAPIGRDAELMCAQLKAAGLNCATC
                     VDVRQLCSDLADGAGAIILTEEALTHESAAELMRELSRQPPWSEIPLIILTGALSFEV
                     KVHSFDGLVRRTNVTLVDRPVRIKSLISAAQTALRARQRQYDVRDLMQQLEERVHERD
                     RFLAVLGHELRNPLGAILLASQMTDPDGLIDPEKAQLIERQSRHLTRLVNDLLDLSRV
                     AAGKIVLKRQRVNLVEIVTQSVRMVVPMAQRQGVDLALRTPENRLIVDADPVRVDQIV
                     TNILTNAIKYTPEGGHVTLDLSDDNGQATIRVTDDGVGVAPERIGGIFELFAQAENAI
                     GRAQGGMGIGLALVRNLTELHDGSVAARSEGVGKGSEFVVRLPLAAYDEHHGSGTAAA
                     PKKRETEVHVARRIAIVEDNKDVRDLLRLKLSRLGHTVVDARDGLEGLRIVLAERPDL
                     ALVDLGLPGIDGFQLAKEVREQLGDDVILVAVSGFGQPDDKRRAIEAGFDEHLTKPAD
                     VHDIENLLMRLPPRNARAAEIV"
BASE COUNT          795 a         1332 c         1283 g          722 t
ORIGIN      
        1 gatatgaaag agaagggctc ggatgtgaat ctggccacag agttgctggt ggatgccttc
       61 tcaaacgcat atgacgcagc ggtgatcatc tccaacgact cggatttggt ggcacccatt
      121 caagctgtca ggaagctcgg aaagccggtc ggcatcatca atcctcacca gcgtcaatcg
      181 ttggagctga tgaaatgcgc cagcttcgtc aaacaggtac gcacatgggc gctcgctcaa
      241 tcgttgctcc cgtccgtact gacagaccgc aatggtgagt ttcacaagcc gcccggctgg
      301 tgagcagaaa gtccttagca ttacccacgc aacaaacccg cttcacacac cccctcgatc
      361 acccgctccc ccgcttcatt ctcgatccac gcttcgatct cgaggcggtg tttcgtgcgg
      421 tcgatcgcgc gaaccacgaa gtgcgcggtg gccgtatcgc cgacgtagac cggcaataga
      481 aaccgcagcg tttgcgagcg gtagatcgca ccggttccgg ggaggaacat gcccaccatc
      541 gtcgagaaga tcgaggcggt gagcattccg tgcaggacgc gccggccgaa cttcgtttcc
      601 gccgcaaagg catcgtcgac gtgaagcgga ttgacgtcgc cggtgatggc gatgaagcgc
      661 tgcacgtcgt cctccgtgaa cgtcttcgtg aagctcgcac gctggccgac ggagaagtcc
      721 tcgatgcgtg gcatgtagcc atgatcatgg cacgcaagtt ggaatgaacg tgctcagccg
      781 cgacggattc gggggacatc gcagccaacg aagccgttca ttttggttcc atcagttgca
      841 actgaggttg ggagcgagtg cccgccgcag gtgcgccatc gcaaagattg ttcgcatggg
      901 agcaggaaca tgaattcgga gttcgcaccc acaggaatcc aggggctcga ctacattctt
      961 ctcggcgggt ttccgcgcaa ccgcgtctat ctcattcagg gcgacccagg agtcggcaag
     1021 acgacgatgg gcctgcagtt cctgctcgaa ggtatgcgcg ccggcgaggc tggtctctac
     1081 atcaccttgt ccgaaacggc tgaggaactg cgcgccgtcg ctacctcgca tggctggtcg
     1141 ctcgacggcc tggccatcta cgaacagatg atcggcgagg aagcgctggc cgaagaagaa
     1201 accacggtct tttatcccgc cgaggtcgag ttgggcgaga cgatcaaggg gatgctgaag
     1261 gaagtcgatc gcgtcaagcc gcggcgcatc gtgctcgatt cactcagcga gatccggctg
     1321 ctcgcccaga gcggacttcg ctatcgaaag cagatcctgg cgctcaagca gttcttctcc
     1381 ggacgcaacg tcacggtcct ctgcctcgac gaccgcacgt cggagttcca ggacatccag
     1441 ctccagagcg tgccgcacgg ggtggtcgag ttggagcggt acacgccggt ctacggcgcg
     1501 gcccggcgac ggctgcaact cgtgaaagtc cgcggtctcg cctttcgcga cggcttccac
     1561 gacttcaacg taatcactgg cggcatcgtc gtctatccgc gcctcgttgc ggcggagcat
     1621 cggacgatcg cgacgcacga gcaggtggcg accgggattc ccgagctcga cacgatggtc
     1681 ggcggtggcc tcgaccgcgg caccagcacg ctgatcatgg gtcctgccgg ctccggcaag
     1741 tccgcgctgg cgacgcagca ggtcgtggcc gctgcaagcc gaggcgagaa ggccgccatg
     1801 ttcatcttcg aggagagtct cggcagcgtc ctggcgcgat ccgcttcgct cggaatgccg
     1861 ctggagccgc tcgtcaacga agggaaggtc acgatccgcc aggtcgatcc ggcgcagttg
     1921 caacctggcg agttcgcgca tctcgtgcgc gaagccgtcg aagatcgcgg cgcgcgcgtg
     1981 ctcgtcatcg acagcttgaa cgggttcctc aacgcggtgc cggaagagcg cttccttctc
     2041 cttcacctcc acgagcttct cagttacctc ggccagaacg gcgtcgccac gatcctcgtt
     2101 ttcgcgcagc acggcctagt cggtcacatg cagtcgactg tcgatgtcag ttacctcgcc
     2161 gatgctgtga ttcttcttcg ttacttcgag gcggcaggcc ggattcacaa ggctatctcg
     2221 gtcgtgaaga aacggagcgg accgcacgag accgcgatcc gttcgatgac gatggggaaa
     2281 ggcgggctga aaatcggacc gccactcgaa aactaccgcg gcgtgatgtc cggcatccca
     2341 acctacgacg gcaccgtcgg tccctcgaga gacgcgtgac tactgaagcg aagcccaagg
     2401 ccagcggtca tcgatccgac cgcattctca tcgtcgcgcc aatcgggcgc gacgcggaac
     2461 tgatgtgcgc gcagttgaaa gcagcaggct tgaactgcgc gacgtgcgtg gacgttcgtc
     2521 aactgtgcag cgacctcgcg gacggcgccg gcgcgatcat cctgacggaa gaggcgctca
     2581 cgcacgagag cgccgcggag ctgatgcggg agctctcccg ccagccgccg tggtcggaga
     2641 ttccgctgat catcctcacc ggtgcgcttt cgttcgaagt caaagttcat tcgttcgatg
     2701 gcctcgtccg ccgcacgaac gtcacgctgg tcgaccggcc ggtgcgcatc aagagcctga
     2761 tcagcgccgc gcaaaccgcg ctccgcgcgc ggcaacggca atacgacgtc cgcgatctga
     2821 tgcagcagct cgaagagcgc gtgcacgagc gcgaccgctt tctcgccgtc ctcggacacg
     2881 agctccgcaa tccgctcgga gcgattctgc tggcgtcgca gatgacggat cccgacggcc
     2941 tgatcgaccc cgaaaaggcg cagctcatcg aacggcagtc gcggcatctc acgcgcctcg
     3001 tcaacgacct gctcgatctc tcgcgcgtcg ccgccggcaa gatcgtgctg aagcgccagc
     3061 gcgtgaatct cgtcgagatc gtcacgcagt ctgtacgcat ggtcgtaccg atggcgcagc
     3121 ggcagggagt cgatctcgcg ctgcgcactc ccgagaatcg gctgatcgtc gatgccgatc
     3181 cagtgcgcgt cgatcagatc gtcaccaaca ttctgacgaa tgcaatcaag tacacgcccg
     3241 aggggggaca cgtcacgctc gatctgagtg atgacaacgg gcaggccacg atccgcgtaa
     3301 ccgacgacgg cgtaggcgtg gcgcccgagc gcatcggcgg catcttcgag ctcttcgcgc
     3361 aggccgaaaa cgcgatcggc cgcgcgcagg gcgggatggg aatcggtctc gccctcgttc
     3421 gcaatttaac cgagctgcac gacggcagcg tcgcggcacg aagcgaaggc gtgggcaagg
     3481 gaagcgagtt cgtggtccgc ctgccgttgg cggcatacga cgagcaccac ggcagcggca
     3541 ccgccgccgc tccgaagaag cgagagacgg aggtgcatgt cgcgcggcga atcgccatcg
     3601 tcgaagacaa caaggacgtt cgcgatctcc tccgactcaa gctcagccgt ctcggccaca
     3661 ccgtcgtcga cgcacgcgat ggcctggaag gactgcgtat cgtcctggcc gagcgccccg
     3721 atctcgccct cgtcgacctc ggccttcccg gcatcgacgg cttccagctg gcgaaggaag
     3781 tccgcgagca gctcggagac gacgtcatcc tcgtcgccgt cagcggcttc ggtcagcccg
     3841 acgacaagcg gcgcgcgatc gaagccggct ttgatgaaca cctgacgaaa ccggccgatg
     3901 tacacgacat cgaaaatctc ctgatgcgcc tgccgccgcg gaacgcgcgc gctgccgaaa
     3961 tcgtgtagtc atcgccgctc ttcggccgac aacggcgttc gaatcgtcac gcgggacgag
     4021 ttgctccctc cgctacaatc cgcgcccatg aagaacgatt cgaccctcct ccgctgcggc
     4081 cggctggcca ttctgatcgt cgcgctagtc gtgctcgccc cctcgctggt cg
//
LOCUS       DATLVW010000809         8073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404166, whole genome shotgun sequence.
ACCESSION   DATLVW010000809 DATLVW010000000
VERSION     DATLVW010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404166"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VNN25_23745"
     CDS             <1..810
                     /locus_tag="VNN25_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HXH94610.1"
                     /translation="DRLATIPAGEERVLLSTGKYIGEGFDDARLDTLMLTLPVSWRGT
                     IAQYAGRLHRLYDSKREVRVYDYADLDVPMLARMFDRRCRGYEVVGYTIVLPASAVPG
                     WPADVPLPADPGWKHDYAASVRRLIRDGVDTPLANLFVHAARRVTPESEGLDRARSAS
                     EAFLYRRLETLSQTRGHFALNANLPIAFDARGQMEVDLLCAGARVVVEIDGAQHLSDP
                     IAYRRDRQKDRLLQENGYWVLRFLAEDVGKELDAVLDAIVRALTKRERPQS"
     gene            complement(827..1507)
                     /locus_tag="VNN25_23750"
     CDS             complement(827..1507)
                     /locus_tag="VNN25_23750"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXH94611.1"
                     /translation="MRLVTWNCCRGAYTRKAPLLEPLAFDVAVIQECAKPSQESDHCL
                     WSGGNPRQGIAVVSREPYRIRAMERRIGVPRYTFPIEVTGPESFLLLAVWSKGQQRHP
                     YVEAVVKAVRLYRHLFTQPVVLMGDLNSNTFWDANHDDDRNHSALVLQLSRLGLVSAY
                     HAFHGEPHGGESQPTYYFQWKESRPFHIDYCFIPDAWSSRVHRVEVGSYGEWKEHSDH
                     RPILVDVR"
     gene            complement(1582..2640)
                     /locus_tag="VNN25_23755"
     CDS             complement(1582..2640)
                     /locus_tag="VNN25_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXH94612.1"
                     /translation="MTLRFGYWMPMFGGWLRNVEDERMEASWDYVRRLAVRSEELGFD
                     LSLLAELNLNDIKGVEAPSLDAWSTAAALAAATRTLELMVAVRPTFHPPALFAKQASN
                     IDHISGGRLALNVVSSWWRDESLQYGVPFDEHDDRYRRTEEWLAVVKGLWQEKRFSYD
                     GRLYRVNDAIVEPKPLRKSLPPIYAGGESSAAKDLIAREADAYVMHGDPPENLVPKIA
                     DMSARRAKLAPGAKRLLFGVAGFVVCRERAEEAKREVERITNVHASAAGNANYEQWLS
                     GTKLEQQVSIQDYSVSNRGLRTNLIGSPEEIAARLVALEQTGIDLVLLQFSPQYEEME
                     RFGEQVIPLVREMSRAVA"
     gene            complement(2637..3614)
                     /locus_tag="VNN25_23760"
     CDS             complement(2637..3614)
                     /locus_tag="VNN25_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94613.1"
                     /translation="MSRSENLTILYEHPHWFNPLFEELERRGIPHERVDAASEAFDPD
                     GYGPDDVVFNRISPSAWTRARGALIFQTLGWTRQLELNGADVINGSAIFELEISKAAQ
                     GAFLQRIGVTTPRTEVTTPAGLGAASERLEFPLLVKPNVGGSGAGVRLLPTRDALLEV
                     VESGAIDRGGDGLLLLQEYHAPAGGFITRVETLDGAYLYGIRIHLGDDGGFDLCPADV
                     CKTTDGAALTSSACPADSHKKGLSVEAFTPPDDVIDAVENIALRSRLDVGGIEYLESA
                     RDGRRYFYDINALSNFVADPLRVIGFDPTARVVDSIEERLQARRQRRVS"
     gene            complement(3981..4331)
                     /locus_tag="VNN25_23765"
     CDS             complement(3981..4331)
                     /locus_tag="VNN25_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94614.1"
                     /translation="MSEPQLGDKETIDILALEYNSLRGHINARMSSMFQLAAVFVASA
                     ALLFRQSIETPVLVTEGIVLVAFGIGLWLLWHDMVKAGRRVQQLETEINRRANEKLLI
                     WESELGGISHGYWR"
     gene            complement(4419..5162)
                     /locus_tag="VNN25_23770"
     CDS             complement(4419..5162)
                     /locus_tag="VNN25_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH94615.1"
                     /translation="MHTQLKVSFVIPAFNEERLLSLALASLSDEIERTGWPAEIIVVD
                     DGSTDSTAEIANAFEVVVVRAPRGGLVNARRLGFLAAKGDLIANIDADTTIPRGWLMQ
                     VLREFERNPSLACISGPYIYKDISELARAAVRLFYCIGFILHLLNQYVLRVGSMVQGG
                     NFVVTRTALSRIGGYSDRFRFYGEDTDLGRRLIAVGRVKFKFGLWASSSGRRLRGEGI
                     VRMGLRYASNFIWATWAHRPFTNDWKDIR"
     gene            complement(5180..5731)
                     /locus_tag="VNN25_23775"
     CDS             complement(5180..5731)
                     /locus_tag="VNN25_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94616.1"
                     /translation="MGQNDETSGEPAFDERSIARRHGFRELIYPDRSAIESVQRFLQE
                     IVSSWTANNPPKIHLTILRRFFQGAIERQNISKMPNMPLEEFLLIQAPYGICSRVFGR
                     ILERKKGAFYQAAYHSVEKCEEFLSYLLDPDTVWIRKSLPEEMIARAIVLQEFLERYP
                     EAKQRSDEDLERRLRARRAGGAG"
     gene            complement(5996..>8073)
                     /locus_tag="VNN25_23780"
     CDS             complement(5996..>8073)
                     /locus_tag="VNN25_23780"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXH94617.1"
                     /translation="SIAVVGAGRKPGQLGHEIVANLQRSGFTGDLYVVNPKANDIDGV
                     RSYSSLRDIEGVVDLAIIVVPAAEVEHVIDDCVEKSVRGAVVITAGFGETGPAGREAE
                     RRLLDKVRAAGMRMVGPNCMGIINTDPAIRMHGTFAAVYPPAGNVAMSSQSGALGVAI
                     LDYARSLNIGFSTFLSVGNKADVSGNDLIQYWAEDPRTDVILLYLESFGNPRKFAEIA
                     RRVGKTKPIILVKSGRSASGARAAASHTGALASSDAIVADLLRQAGVIRTDTLEELFD
                     VASLLANQPLPRGRRVAILTNAGGPGILAADACEARGLTLPPLAGSTVTQLRSFLPAA
                     ASVGNPVDMIASASPEHYKKSLEALLADPSIDNVLVIYIPVVPTQAPDVAAAIRETAT
                     RAGGKTILATFMSAHGVPPSLEPVPAFPFPERAVGALSRAVGYAEWRRRPEEQAAELN
                     LDTIALRSIVDNALERGGGWLGPNEIEHLLRASGIETAGGEFVSDAEEAFEAAVRIGK
                     PVALKAIGPKLLHKSDRGGVRLSLKNECDAQEAFLEMKTLLGDDMEAAFVQPMVTGGV
                     EIMLGATNDPTFGHVLAFGAGGTLVEVLSDVAFRIHPLTPNDAEDMINELRCAKMLGG
                     VRGAAPSDVPALRQAIMRLSALISACPEIQELDINPLKVLEKGAIALDARVRVERLAP
                     APPSRRIAY"
BASE COUNT         1484 a         2488 c         2543 g         1558 t
ORIGIN      
        1 gataggctgg caaccattcc ggcgggtgag gaacgcgtct tgctttcaac cggcaagtac
       61 atcggggaag ggttcgacga tgcgcggttg gacactctca tgcttacgct tcctgtgtcg
      121 tggcgcggga cgatcgccca atacgcaggc cggctgcacc gcctgtacga cagcaagcgc
      181 gaggtcaggg tgtacgacta cgcggatctc gatgtgccga tgctggcgcg aatgttcgac
      241 cgtcgttgcc gcggatacga agtcgttggc tatacgatcg ttcttccggc aagcgccgtc
      301 cccggttggc ccgcggatgt gcctcttccc gccgatccgg gctggaagca cgactacgcc
      361 gccagcgtgc gccggctgat tcgtgacgga gtcgacacgc cgctggcgaa cctgtttgtt
      421 catgcagctc gccgcgttac tccggaatcc gagggactcg accgcgcgcg cagtgcgagc
      481 gaggcgttcc tctatcgccg actggagact ctgagtcaga ctcgggggca ttttgcgctg
      541 aatgccaatt tgccgatcgc gttcgatgcc cgcggccaga tggaagtcga tctcctgtgc
      601 gccggcgcac gtgtcgtggt cgaaatcgac ggcgcacagc acctctcgga tccaatcgcc
      661 taccggcgtg accggcaaaa ggaccggttg ctccaggaga acggctattg ggtgttgcgc
      721 tttctggccg aggatgtagg gaaggagctc gacgcggttc tggatgcgat cgtacgggcg
      781 ttgacaaagc gagagcggcc tcagtcatga cacagcaaca cggtgatcac ctgacatcga
      841 caagaatcgg ccgatggtcg ctgtgctcct tccactctcc ataggagccg acctcgacgc
      901 gatgaacgcg cgacgaccag gcgtcgggga tgaagcaata gtcaatgtga aacggccggc
      961 tctccttcca ctggaagtag tacgtgggct gactctcgcc tccgtggggc tcgccgtgga
     1021 aggcgtgata ggcgctgacc agccccagtc tcgatagctg caggaccaac gcgctgtggt
     1081 tgcgatcgtc atcgtggttc gcgtcccaga atgtgttcga gttcaggtct cccatcagca
     1141 caaccggctg cgtgaagagg tggcgataga gcctgaccgc cttgacgacg gcctcgacgt
     1201 acgggtggcg ctgctgtccc ttcgaccaga cggccagcag cagaaagctc tccggtccgg
     1261 tcacttcgat gggaaacgtg tatcgcggaa ccccgatgcg ccgctccatc gcgcggatgc
     1321 ggtacggctc gcgggaaaca acggcgattc cctgccgcgg gttgcctccg gaccagaggc
     1381 agtgatccga ctcctgcgaa ggcttcgcgc actcctgaat cacagcgaca tcaaacgcga
     1441 gcggttccaa caaaggagcc ttccgcgtat acgcacctcg gcagcagttc catgtgacca
     1501 atcgcatcgt cggctttagg ctcaacaaag gttaccaatc cctctgatct gtgaacatct
     1561 gcgacatctg cggatgaagt ctcacgccac cgcccgcgac atctcccgca ccagcggaat
     1621 cacctgctct ccgaaacgct ccatctcctc gtactgcggg ctgaactgga gcagcaccag
     1681 gtcgatgccg gtctgctcca gcgccacgag gcgtgcggcg atctcctccg gacttccgat
     1741 caggttcgtg cggaggccgc ggttcgatac cgagtagtcc tggatcgaca cctgctgctc
     1801 cagcttcgtg cccgaaagcc attgctcgta gttggcgttg ccggcggcgc tggcatgcac
     1861 gttcgtgatt cgctccacct cccgcttcgc ctcctcggcg cgctcgcggc agacgacgaa
     1921 gccggccacg ccgaagagca gtcgtttcgc accgggcgca agctttgcgc ggcgtgcgct
     1981 catgtcggcg atcttcggca ccagattctc cggaggatct ccatgcatga cgtacgcatc
     2041 cgcttcgcgc gcgatcagat ccttcgccgc ggacgactct cctcccgcgt aaatcggcgg
     2101 caacgacttg cgcagtggtt tcggctcgac gatggcgtcg ttcacgcgat acagccggcc
     2161 gtcgtacgag aaacgtttct cctgccacag ccccttcacg accgccagcc actcctcggt
     2221 gcgccgatag cgatcatcgt gctcgtcgaa cggcacgccg tactgaagcg actcatcgcg
     2281 ccaccacgac gagaccacgt tcagagccag gcgtccgccc gagatgtgat cgatgttcga
     2341 ggcctgcttg gcgaagagcg cgggcggatg gaacgtcggc cgaaccgcca ccatcagctc
     2401 gagcgtccgc gtcgcggcag caagcgccgc ggccgtcgac cacgcatcga ggctcggcgc
     2461 ttccacgccc ttgatgtcgt tgagattcag ctccgcgagc agcgagagat cgaagcccag
     2521 ctcttcgctg cgcactgcca gccgccgcac gtaatcccaa ctggcctcca tccgctcgtc
     2581 ctcgacgttg cgcagccagc caccgaacat cggcatccag tatccgaagc ggagcgtcat
     2641 gacaccctcc gctggcgccg tgcctgaagg cgctcttcga tggagtcgac gacgcgcgcg
     2701 gtgggatcga agccgatgac ccgcaacgga tcggcgacga agttcgacag cgcgttgatg
     2761 tcgtagaagt agcgccgccc gtcgcgcgca ctctccagat actcgatgcc gccgacgtcc
     2821 agccgcgacc gcagagcgat gttctcgacc gcgtcgatga catcgtccgg cggcgtgaag
     2881 gcctcgacgc tcaatccttt cttgtggctg tcggcggggc aggcgctgct ggtgagcgcg
     2941 gcgccatcgg tggtcttgca gacgtcggcg gggcagagat cgaatcctcc atcgtcgccg
     3001 aggtgaatgc ggatgccgta gaggtaggcg ccgtcgagcg tctccacgcg ggtgatgaag
     3061 ccaccggcag gcgcgtggta ctcctgcagc aacagcagcc cgtcgcctcc tcggtcgatc
     3121 gctcccgatt cgacaacctc cagtaatgca tcgcgcgttg gcaggagccg cacgccggcg
     3181 ccgctaccgc cgacattcgg tttgacgagg agcggaaatt cgagccgttc gctggccgcc
     3241 cccagtccgg ccggtgtcgt cacttccgtg cgcggcgtgg tgacgccgat gcgctgcaga
     3301 aacgcgccct gggctgcctt gctgatctcc agctcgaaga tggccgagcc gttgatcaca
     3361 tcggcgccgt tcagctccag ttgccgcgtc catccgaggg tctggaagat gagcgcgccg
     3421 cgagcacggg tccaggcgga agggctgatg cggttgaaga cgacgtcgtc cggtccatag
     3481 ccgtccggat cgaacgcctc ggacgccgca tcgacgcgct cgtgagggat tccgcgccgc
     3541 tccagctcct cgaacaacgg attgaaccag tgaggatgct cgtaaagaat ggtgaggttt
     3601 tccgaccgtg acatcgcgct ctcctttgag tccgcggccg gccggttgga gcgtgaggtc
     3661 gaaaatgaaa aaccccgcga cgaacaggcc gtgcggggtt ggttgttgat gaagtgtgtc
     3721 gtctaaatca ggacgccaag cctctcccgc atgtcgtagg acagcaacac atcgacatcg
     3781 cgagagactg gtgcgtcatt ggcgaggcgt tatacgcgga ggtggagggg gttgtcaaat
     3841 gggggctctt gggggtgcag cgtgatgtcg gtggtccggg atccgcctcg ttcgcaccgt
     3901 gctctcgtga tctacatttg aacgacgatg agccaacggc cggggacacc gtacgcattc
     3961 tctctgggag gaacgaatgg ctatcgccaa tatccgtggg agattccgcc cagctcactt
     4021 tcccagatca agagcttctc attcgctcgt cgatttatct cagtctccag ttgctggact
     4081 cgtcggccgg ccttaaccat atcgtgccat agaagccaga gcccaatacc gaaagctacg
     4141 aggacgatcc cctcagtcac aaggacggga gtttcgatcg attggcgaaa aagcaaagcg
     4201 gcactcgcca cgaagacggc cgccaactgg aacatgctcg acatgcgcgc attgatgtgg
     4261 ccgcgcagtg agttgtactc tagtgctagg atatcgattg tctctttgtc gccgagttgt
     4321 ggctcactca ttgtcgctct cccgcttttc tggctctgct atctaaatca gattctcttt
     4381 tctgcgtacg gctcgaattg cattttcgac gacaatgatc agcggatgtc cttccaatcg
     4441 ttcgtaaatg gcctgtgcgc ccatgttgcc cagatgaagt tggaggcgta gcggagaccc
     4501 attcgaacga ttccttctcc tcgaagccgt ctgcccgacg acgaggccca tagaccaaac
     4561 ttgaatttga cgcggccgac agcaataagg cggcgtccga gatcagtgtc ctccccataa
     4621 aacctgaagc ggtcactgta cccgccgatc cggctaagcg cagttctcgt tacgacaaaa
     4681 ttgccacctt ggaccatgga gcccacccga agcacatact gattcagaag gtgaagaata
     4741 aaaccgatac aataaaacag tctgacggca gctctagcca actccgatat gtccttgtaa
     4801 atgtaagggc cgctgatgca agctagtgag gggttgcgct caaattcgcg aagcacttgc
     4861 attagccaac cccgaggtat ggtcgtgtct gcatcgatgt tcgcgatcaa gtctcctttt
     4921 gcggcgagaa atccgagcct acgtgcattg accagtccgc ctcgtggtgc tcgtacgaca
     4981 accacctcga atgcgttcgc gatttctgcg gttgagtcag tgctgccgtc gtcgacaaca
     5041 atgatctcag cgggccaacc ggttcgctcg atttcgtccg aaagagatgc caatgcgagt
     5101 gacaacagcc gctcttcatt gaaggctggt atgacaaaac tcactttcag ttgagtatgc
     5161 atctggtcgg tctccccggc tatccggcgc cgcccgcgcg tcgcgcccgg agccggcgtt
     5221 ccagatcctc atcggagcgc tgcttggcct cgggatagcg ttccaggaat tcctgcagca
     5281 cgatggcgcg cgcgatcatc tcctcgggga ggctcttgcg aatccagaca gtgtccgggt
     5341 cgagaaggta ggaaaggaac tcctcgcatt tctccaccga gtgataggcg gcctgataaa
     5401 aagcgccttt cttgcgctcg aggatccgtc cgaagacgcg cgagcagatg ccgtatggcg
     5461 cctgaatgag caggaactct tcgagcggca tgttcggcat cttgctgatg ttctgccgct
     5521 cgatggctcc ctggaagaag cgccgcagga tggtgaggtg gatcttggga ggattgttcg
     5581 ccgtccacga agagacgatc tcctggagga aacgctggac gctctcgatg gcagaccgat
     5641 cgggatagat cagttcgcgg aagccatgcc tgcgcgcgat cgaccgctca tcgaacgctg
     5701 gttccccgct cgtctcgtcg ttctggccca cgaaccgcgc tcctcctttc ctggttatgg
     5761 ttatacatca cgatggtggt aaccatcatc gccgccagca tggtcgcctg gatgcatgta
     5821 cgggcaggga aaggtcgttc acagcgagat cttcgagctt tcaaaatggc gtcaggtccg
     5881 aaattgaaag tgacggagcc ctggtccgat cccagaaccg cagtgcatcc gtcactttca
     5941 atttcagacc tgaccccatt ttgaaagctg cgcgatgcgg gcggggctgg accagtcagt
     6001 aggcgattct tcgcgatggc ggcgcgggtg ccagtcgttc gacgcgcacg cgggcgtcga
     6061 gagcgatggc gcccttttcg aggactttga gcgggttgat gtctagctcc tggatttcgg
     6121 ggcacgcgct gatcaacgcc gagaggcgca tgatcgcctg gcgcagggcg gggacatcgc
     6181 ttggtgccgc gccgcggacg ccgcccagca tctttgcgca gcgtagttcg ttgatcatgt
     6241 cctcggcgtc gtttggggtg agggggtgga tgcggaaggc tacgtcggag aggacttcga
     6301 cgagcgtgcc gccggcgccg aacgctaaga cgtggccgaa ggtggggtcg ttggtggcgc
     6361 cgagcatgat ttcgacgccg ccggtgacca tgggctggac gaacgcggct tccatgtcgt
     6421 cgccgaggag ggtcttcatt tcgaggaagg cctcttgcgc gtcgcactcg tttttcagcg
     6481 acagcctgac gcctcctcga tcgctcttgt gaaggagttt gggaccgatc gccttgagcg
     6541 cgaccggttt gccgatgcgc accgccgctt cgaatgcctc ctcggcgtcg gagacgaatt
     6601 cgccgcctgc tgtctcgata cccgaggcgc gaaggaggtg ttcgatctcg ttcgggccga
     6661 gccagccgcc gccgcgctcg agcgcgttgt cgacaatgga ccggagcgcg attgtgtcga
     6721 ggttcagctc cgcggcttgc tcttccgggc ggcgtcgcca ttcggcgtag ccaactgctc
     6781 tggacagcgc gccgactgcg cgttccggaa acgggaacgc aggaactggc tcgagcgacg
     6841 ggggaacgcc gtgagcgctc atgaacgtcg ccaggatcgt cttgccgccg gcgcgcgtgg
     6901 cggtctcgcg aatcgcggcg gcgacgtcgg gcgcctgtgt cggcacgacc gggatgtaga
     6961 tcacgagcac gttgtcgatc gacggatcgg cgaggagcgc ttcgagcgat ttcttgtagt
     7021 gctccggcga cgcagacgcg atcatgtcga caggatttcc gacgctcgcg gccgccggaa
     7081 ggaacgagcg aagttgcgtc accgtcgatc ccgcgagcgg cggcagagtc agaccgcgcg
     7141 cttcgcaggc atcggcggcg aggatgcccg ggccgccggc gttcgtgagg atggctacgc
     7201 ggcgcccgcg cgggagcggc tgattggcaa ggagtgaggc gacatcgaac agctcttcga
     7261 gcgtgtcggt gcggatcacg cctgcctggc gcagcaggtc ggcgacgatg gcatcgctcg
     7321 acgccagagc gccggtgtgc gaggccgcgg cgcgcgcacc cgaagcggag cggccggatt
     7381 ttacgaggat gatcggcttg gtcttaccga cgcgacgcgc gatctccgcg aacttgcgcg
     7441 gatttccgaa gctctcgaga tagagcagga tcacatcggt ccgaggatcc tcggcccagt
     7501 actgaatgag gtcgttgccc gaaacgtcgg ccttgttgcc gacggagagg aacgtcgaga
     7561 agccgatgtt cagcgatcgt gcgtagtcga gaatggccac gcccaacgcg ccgctctggc
     7621 tcgacatggc cacgttgcct gccggcggat agaccgcggc aaacgtgccg tgcatccgaa
     7681 tcgcggggtc ggtgttgatg atgcccatgc agttcggacc gaccatgcgc atgccggcgg
     7741 cgcgcacttt gtcgagaagg cgtcgctctg cctcacggcc agccgggccg gtctcgccga
     7801 agcctgccgt aatcacgacg gcgccccgca ccgacttctc cacgcaatcg tcgatgacgt
     7861 gctccacctc ggccgccggg acgacgatga tggccagatc gacgacgcct tcgatgtcgc
     7921 gcagcgagga atacgagcga actccgtcga tgtcattcgc ttttggattt acgacgtaga
     7981 ggtcgccggt gaatcccgat cgctgcaggt tcgcgacgat ctcgtgtccg agctggccgg
     8041 gcttccgtcc ggcaccgacg acggcgatcg atc
//
LOCUS       DATLVW010000810         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_33039, whole genome shotgun sequence.
ACCESSION   DATLVW010000810 DATLVW010000000
VERSION     DATLVW010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33039"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VNN25_23785"
     CDS             <1..461
                     /locus_tag="VNN25_23785"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXH94618.1"
                     /translation="NILFICAGAFSDIDDNSDAKPIGFFGDGDVPAKDISTEQLVKYG
                     FLPELLGRLPVHVELDPLTADDLVTILTEPREAMIPEYQRLCALDQIQLEFSAGALYA
                     IAEAALKQKLGARALRAILEKVLHPVLFTGPERAGDRVVIEPEDVRRVIA"
     gene            471..1325
                     /locus_tag="VNN25_23790"
     CDS             471..1325
                     /locus_tag="VNN25_23790"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXH94619.1"
                     /translation="MQTIGISAKTTSEDALAFAARVTADLRGRGYTVCFDYGTADKLG
                     DRGKCVAKSDLGKHTELLITFGGDGTLLSVARHAPKDVPILGVNMGTLGFLTEVRVED
                     FAEVIERVLADDFESEKRVTFDVEVRGPGRDHRKYRVLNDATINKSALARIIEMRVTV
                     GGQFVSSFRADGLIIATPTGSTAYNLSAGGPIIYATMGAVVLTPICPHMLSNRPIVLP
                     DDLEIEIGIATPNQEIFLTLDGQEGLPIAQNDKVCINKSPNPVTLVRTSKTSYFDVLR
                     TKLKWGEG"
     gene            1387..1830
                     /locus_tag="VNN25_23795"
     CDS             1387..1830
                     /locus_tag="VNN25_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020583637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HXH94620.1"
                     /translation="MPTENQILGQKGEELVVKSCHCPRCKRKRTLKRLPKNFKCADVV
                     CDFCGYLAQVKTATAKSVDHLPDRILGAAWGVQSERMAAGIYLPLFIVLVTGRQSSIF
                     YLSTDAQTPEMFVARKPLSEKARRAGWQGFVYDLRKVRSSAIRLA"
BASE COUNT          525 a          804 c          713 g          477 t
ORIGIN      
        1 ccaacattct cttcatctgt gccggcgcgt tcagcgacat cgacgacaac agcgacgcca
       61 agccgatcgg cttcttcggc gatggcgatg ttcccgcgaa agacatctcc accgaacagt
      121 tggtcaagta cggcttcctg cccgagctcc tcggacgcct tcccgttcac gtcgagctcg
      181 atcccctcac cgctgacgat ctcgtcacca tcctcaccga gccacgcgag gcgatgatcc
      241 ccgagtacca gcgcctctgc gcgctcgacc agattcagct cgagttctcc gccggcgcgc
      301 tgtatgcgat cgccgaggcg gcgctgaaac agaagctcgg tgcgcgggcc ttgcgcgcca
      361 tcctcgagaa ggtgctccat ccggtgctct tcaccggtcc cgagcgcgct ggcgaccgcg
      421 tcgtgatcga gccggaagat gtgcgaagag tgatcgcgta aactcttccc gtgcagacca
      481 tcggcatctc cgcgaagacg acctcggagg acgcactcgc tttcgcggcg cgcgtgaccg
      541 ccgatcttcg cggccgcggc tacaccgtct gcttcgacta cggcaccgcc gacaagctcg
      601 gcgatcgcgg caagtgcgtg gccaagagcg acctcggcaa acacaccgag ctgctgatca
      661 cgttcggcgg cgacggcacg ctcctctccg tggcccggca cgctccgaag gatgtgccca
      721 tcctcggcgt gaacatgggc acgctcggct tcctcaccga ggtgcgagta gaggactttg
      781 cggaggtcat cgagcgggtg ctcgcggatg atttcgaaag cgagaagcgc gtcaccttcg
      841 acgtggaggt acgcggaccg ggccgcgatc accgcaaata ccgcgtcctc aacgacgcca
      901 cgatcaacaa aagcgcgctg gcccgtatca tcgagatgcg cgtcacagtc ggcggccagt
      961 tcgtttcatc gttccgcgcc gacggcctga tcatcgccac cccaaccggc tccaccgcct
     1021 acaacctctc cgccggcggc ccgatcatct acgccacgat gggcgccgta gtgctgaccc
     1081 cgatctgccc ccacatgctc tccaaccgcc ccatcgtcct ccccgacgac ctcgagatcg
     1141 agatcggcat cgccaccccc aaccaggaaa tcttcctaac cctcgacggc caggaaggcc
     1201 tccccatcgc gcagaacgac aaagtctgca tcaacaagtc ccccaacccc gtaacgctag
     1261 tccgcacatc aaagacaagt tacttcgatg tgctgcggac gaagttaaag tggggggagg
     1321 ggtgaggggg aagcgataga ggtgaatacg ccaacgcgaa ctgtcgcaat aaccccgatg
     1381 ttaagaatgc caaccgaaaa tcagattcta gggcagaaag gtgaagaact cgtagttaag
     1441 agctgtcact gtccccgttg taagcggaaa cggacgctga agcgactgcc gaagaatttc
     1501 aagtgtgccg atgtggtgtg tgacttctgc ggttatctgg cgcaggtgaa aaccgccacg
     1561 gccaagtcag ttgaccatct gccggaccga attctcggcg ctgcgtgggg cgtgcagagc
     1621 gaacggatgg cggccggcat ctatctccct ctgtttatcg ttcttgtaac gggtcgccaa
     1681 tcgtcaatct tctatttatc aacagacgct cagacgcccg agatgttcgt cgcgcgaaag
     1741 ccgctctctg agaaagcgcg tcgcgcaggc tggcaaggtt ttgtgtacga tcttcggaaa
     1801 gtgcggtcgt cggccattcg cttagcataa atctacgact cgccatggag ggcttcacta
     1861 atgcgtgatg actcgattgt tgaagagacg cgtgctgcaa ggcgtgagct ccatgctgaa
     1921 ttcggcggag acatggtggc cctttggaag tatctgaaaa ccatcgaggc cgagaatttc
     1981 gatcgcgtcg tgaaactgga gccgaaacca gcgattacgc ctactgtcat cccgaaccgc
     2041 cgaagccgtt ggcgatacgc gcaaaccgtg ttgtgtcatc ctgagccgcc gaagccgccg
     2101 caagatttcg taacacgcgc aaccggcaga catcggcggc ggaggacggt cgaaggatct
     2161 caaaatgcga agatctcgtt cgagcgtctt tgatcacgat cgctcgcgct gggcttgcac
     2221 gatctgcgaa gtttgagatc cttcgacgtc ctccgccgcc gagcacgtgc tgcgcaaaca
     2281 tcgcgattcg tgcgggcggc tccggcagtc tcaggatgac acgcaatctc gagtggacta
     2341 tcacttcgga tgacactcaa ccctaacctg catttcgcac gcttgttgcg gattgcgttg
     2401 ttaaaaggtg cgtgagaagc gcggactagc gtcacccgcg cgcactcatt cagcgtgccc
     2461 ccaccttttt caacaaccca atctgccccg cgtgatacag gttgtgctgg atcacgcca
//
LOCUS       DATLVW010000811         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_368165, whole genome shotgun sequence.
ACCESSION   DATLVW010000811 DATLVW010000000
VERSION     DATLVW010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368165"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VNN25_23800"
     CDS             <1..982
                     /locus_tag="VNN25_23800"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXH94621.1"
                     /translation="FASGFQANAEPDPVAATIRHGLLHEALGGASASVQGLLNVHHMG
                     GSAIARWGTREQKEQWVPRLVSGEVLAAIAITEPHVGSDASAVATTATADGTDYVING
                     TKRWITCGQNADVFVVLASGPTAFIVPRRTEGLTIEPIRGMLGCRGYMLAELRFDNCR
                     IPGDHLLGRPGFGLTHVVATGLDSGRYNLAWGCVGLARACLDASFDYSRQRRQFGSAL
                     SEFQLVQQLISKMMTDVEAARLLCFRAGTLRGRRDPAAIKLSAMAKYFASTMVNRVAY
                     DAVQIHGASGVGPDLPLQRYLRDARIMEIVEGSTQILEIAIARYGYQERG"
     gene            979..2112
                     /locus_tag="VNN25_23805"
     CDS             979..2112
                     /locus_tag="VNN25_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94622.1"
                     /translation="MTITRTFDLSRVGESGIHAIVDALRVDHENVRGHQLDTSTGRLT
                     LELTSEEAGQHVEAFIAGMKRTHKLIGRRIVKETHAANLRIDDIDAALASSPDVQILG
                     DGLIGLRGSLLALFRFFEQRFRQIATDFGAEEQHYPVMVPSDLLAEVGYLGHFPQHIT
                     FCSHLPGSLPVLQAVAAEPDTLVERLESPRHVLTPAVCLPCYRQHRGAVIDGVRTLTM
                     QNHVFRYEGTNFRPLSRGWDFTVRDIVFFGSYEDLSRLRVDVMERAVALCRELDLEVT
                     IELANDPFFLDTSRDKAVYQRMGEVKYELLFPIPHRGESLAASSFNLHRDFYTSVYDT
                     RRADGERAESACMGFGVERWVYGFLTQKGMDPKDWPIPVAGKA"
BASE COUNT          407 a          794 c          766 g          402 t
ORIGIN      
        1 tttcgcgagc gggtttcaag cgaacgcgga gcccgatccg gtcgccgcca cgatccgtca
       61 cggcctgctg cacgaggccc tcggcggcgc gagcgcgtcg gtgcaggggc tcctcaacgt
      121 tcatcacatg ggcggcagcg ccatcgcgcg atggggaacg cgcgaacaga aagaacagtg
      181 ggtgccgcgc ctcgtcagcg gcgaggtgct ggcggcgatc gcgatcaccg agccgcacgt
      241 cggcagcgac gcctcggcgg tcgcgaccac cgcgacggcg gacggaacgg actacgtgat
      301 caacggcacg aagcggtgga tcacctgcgg ccagaacgcg gacgtgttcg tcgtgctcgc
      361 gagcgggccg accgccttca tcgtcccgcg ccggacggaa ggactgacca tcgagccgat
      421 ccgcggcatg ctcggctgcc gcggctacat gctcgccgag cttcgcttcg acaactgccg
      481 cataccgggt gatcaccttc tcggtcgccc cggcttcgga ctgacacacg ttgtggcgac
      541 cggtctcgat tcggggcggt acaacctcgc ctggggctgc gtcggtctgg cccgggcctg
      601 tcttgacgcc tcgttcgact actcacgcca gcggcggcag ttcggcagcg cgctgtcgga
      661 atttcagctc gtgcagcaat tgatcagcaa gatgatgacg gacgtggaag ccgctcgcct
      721 gctctgcttc cgcgccggca cgctgcgcgg acgacgcgat cccgccgcga tcaagctgag
      781 cgcgatggcg aagtacttcg cctcgacgat ggtcaatcgg gtcgcgtacg acgccgtgca
      841 gattcacggc gcgagtggcg tcggtcccga ccttccgctc cagcgctatc tccgcgacgc
      901 gcggatcatg gagatcgtgg aggggagcac gcagatcctc gagatcgcca tcgcccgcta
      961 cggttatcag gagcgcggat gacgatcacc aggactttcg atttgagccg cgtcggcgag
     1021 agcggcatcc atgcgatcgt cgatgcgctc cgcgtcgatc atgaaaacgt gcgcggtcat
     1081 cagctcgata cttcgaccgg ccgtctcact ctcgagctca cgagcgaaga ggcgggccag
     1141 catgtggaag cgttcatcgc cggcatgaag cggacgcaca aactgatcgg ccgcaggatc
     1201 gtgaaggaga cccacgccgc gaacctcagg atcgacgata tcgacgccgc gctcgcctca
     1261 tcgcccgacg tgcagatcct cggcgacggt ctgatcggac tgcgcggctc gcttttggcg
     1321 ctctttcgtt tcttcgagca gcggttccgg cagatcgcga cggacttcgg tgcggaggag
     1381 cagcactatc cagtcatggt gccgagcgac ctgctcgccg aagtgggcta cctgggccac
     1441 ttcccgcagc acatcacctt ctgctcgcat ctccccggat cgttgccggt gctgcaagcc
     1501 gtcgccgccg agccggacac gctcgtcgag cgcctcgaat caccgcgcca cgtcctcacc
     1561 cccgctgtct gcctcccttg ctacaggcag catcgcggag cggtcatcga cggcgtgcgc
     1621 acgctgacga tgcagaatca tgtcttccgc tacgagggta cgaacttccg tccgctgagc
     1681 cgcggctggg acttcaccgt tcgcgacatc gtctttttcg gaagttacga ggatctgtcg
     1741 cggctgcgcg tcgacgtcat ggagcgcgcc gtcgcgctgt gccgcgagct tgatctggaa
     1801 gtgacgatcg agctggcgaa cgatccgttc ttcctcgaca cgagccgcga caaggccgta
     1861 tatcagagga tgggcgaggt caaatacgaa ctgcttttcc cgatcccgca ccgcggcgaa
     1921 tcgcttgccg cgtcgtcgtt caatcttcac cgcgacttct atacgtcggt ctacgacaca
     1981 cgccgcgcgg acggcgagcg ggccgagagc gcgtgcatgg gcttcggcgt ggagcggtgg
     2041 gtgtacggct ttctgacgca gaaggggatg gatccgaagg actggccgat cccagtcgca
     2101 ggcaaggcct gacccggcct aacccctcac gctgccccat cggaccgcag ttcggacacg
     2161 aattaccgca acacggtcac ctcgggccgc agtgcggatg cgggcgttca gcaacgcagt
     2221 cacctcgcac cgcaatgcgg ccctgacgga cagcaatgcg gtcatctcgc gccgcacgtg
     2281 cggacaggaa gagccgcaat gcggtcaact agcgccgcac gtgcggacac atcgtgccgc
     2341 aacgttgcgc ctcggcgcgc acgtgcgcg
//
LOCUS       DATLVW010000812         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_322890, whole genome shotgun sequence.
ACCESSION   DATLVW010000812 DATLVW010000000
VERSION     DATLVW010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322890"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..>2797)
                     /locus_tag="VNN25_23810"
     CDS             complement(212..>2797)
                     /locus_tag="VNN25_23810"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH94623.1"
                     /translation="LRPPIARIDGVASVEVLGGQLREYHVLIDRSRLEARGVSLQQVS
                     DAVRGSNVINSPGLINENHQLELALVSGQATSLDDLAAIVVTTVNNVPVRVGDVATVS
                     PGLEPRFTIVTADGRPAVLLNVLRQPTANILSVADGVKAELAALRKQLPRDVEIKPYY
                     DQSLLVSGSVSSVRDAILIGLILSVAILYGFLRNWGTTLVATLVIPVTVLVTFVAMWI
                     VGLSFDLMTLGGVAAAIGLVIDDAIVVVENIYTHMLRGESRADAVHRAISEISGPIIG
                     STITPVVVFLPLALLTGVTGVFFRSLALTMSVALLTSLFLALTFTPVLAEQFVHVKKR
                     DREVSDGADHESEDEHGPLLTRLVNAYEWLLEHALARRPMVLVVIAVLAVLTFFIYKG
                     LGSEFLPEFEEHGFILDYNGPPGASLDEMDRMLGHVERMLKENPGVDSYSRRTGAQLG
                     LAGITESNRGDFAIKLKDSAATDDVVSDLRHKIESSEPALRVEFPGILSDLIGDLVSA
                     PQPIEIRIFSEDAAALRRTAEQIAASIAKVDGVVDVFNGVVISGPAVIFQIDPQRAAM
                     YGVTAADVNEAMTTALGGDVVSNVIEHGRTVGVRVLLGGPRPQLADLRALPIRAPSSN
                     SYVRIDQIAMINYESGQTELEREGLRQSLSVTARLEGRDLGSAIADIQSKLGRELHLP
                     AGTTIEYGGLFQEQQSSFRELLIALGLAIALVFLVLVVEFRSFAHPAAIVSGAVLALS
                     GSLAALLITHTTLNVVSMMGLIMIVGIVSKNGILMLDTVEDHLRGGEDLTTALRRSGR
                     RRFRPVLMTSLAAMLGMLPLALALGSGSELLQPLAICVIGGLLFALMLSLVVTPTIYA
                     MIRGE"
BASE COUNT          559 a          887 c          882 g          469 t
ORIGIN      
        1 gcacagacaa gagtgtctgt gccacacaac cctcgatctt aggcatgccc cttggccaga
       61 ccccgtttgc cgcttccaag accttggttg aatcagaacc gcagcatctc gcagcacaaa
      121 cattcttgtc cgtgctctcc aattactccc ggagcaatgt ggcacagaca ctcttgtctg
      181 tgctcctcaa ttttgtctgg gctcgtccga atcactctcc ccgaatcatc gcgtaaatcg
      241 tcggagtcac taccagcgaa agcatcagcg cgaagagcag tccgccgatc acgcagatgg
      301 ccagtggttg cagcagctcc gagcctgaac cgagggccag ggctagtggc agcatcccga
      361 gcatcgcggc gagggaggtc atcagcaccg ggcggaagcg gcggcggccg gagcggcgca
      421 gtgcggtcgt cagatcttcg ccgccgcgga ggtgatcttc gaccgtgtcg agcatcagga
      481 tgccgttctt cgagacgatg ccgacaatca tgatcaggcc catcatcgag accacgttca
      541 gcgtcgtgtg cgtgatcagc agcgcggcca ggctgcctga cagcgcgagc acggcgccgc
      601 tgacgatcgc ggcgggatgc gcgaatgagc ggaactcgac gacgagcacg agaaagacca
      661 gcgcgatggc gaggccgagg gcgatgagca attcgcggaa ggagctttgc tgctcctgaa
      721 acaggccgcc gtactcgatc gtcgtgccgg ccggcaggtg cagctcgcga ccgagtttcg
      781 attgaatgtc ggcgatcgcg gagccgaggt cacgaccttc gaggcgggcg gtgacggaga
      841 gagattgacg cagcccttcg cgctcgagct ccgtctggcc ggattcgtaa ttgatcatcg
      901 caatctgatc gatgcgcacg tagctattcg acgatggcgc gcgaatcggc agcgcgcgca
      961 gatcggcgag ttgcggacgc ggcccgccga ggaggacgcg tacgccgacg gtgcgcccgt
     1021 gctcgatgac gttcgacacg acgtccccgc ccagcgccgt agtcatcgcc tcgttcacat
     1081 cggcagcagt cacgccgtac atcgcggcac gttgcggatc gatctgaaaa ataacggcgg
     1141 gaccgctgat gacgacgccg ttgaagacgt cgacgacacc gtcgactttt gcgatcgacg
     1201 ctgcgatctg ctccgccgtc cgccgcaacg ccgcggcatc ctcgctgaaa atacggatct
     1261 cgatcggttg cggagcgctg accagatcgc cgatcagatc ggagaggatg cctggaaact
     1321 cgacgcgcag cgcaggctca ctcgattcga tcttgtggcg gagatcgctg accacatcat
     1381 cggtcgccgc ggaatccttc aacttgatgg cgaagtcccc gcggtttgat tcggtgatcc
     1441 ccgcgaggcc gagttgcgcg cccgtgcgcc gcgagtagct gtccacgccc gggttctctt
     1501 tcaacatccg ttcgacgtgt cccagcatcc ggtccatctc atcgagcgac gcgcccggag
     1561 gtccgttgta atcgaggatg aagccgtgct cctcgaactc ggggagaaac tcggaaccga
     1621 ggcctttgta gataaagaac gtgaggactg cgagcaccgc gatgacgacg agcaccatcg
     1681 gccgccgcgc cagcgcgtgc tcaagcagcc actcgtacgc attgacgaga cgcgtaagca
     1741 acggtccgtg ttcatcctcg gactcgtgat cggcgccatc cgaaacctcg cgatcgcgct
     1801 tcttcacgtg aacgaactgc tccgccagca ccggagtaaa cgtcagcgcg aggaacaacg
     1861 acgtcagcag cgccacgctc atcgtcagcg ccagcgatcg aaagaagacg cccgtgacgc
     1921 cggtcagcag cgcgagcggc aggaagacga cgaccggcgt aatcgtcgag ccgatgatcg
     1981 gtccggatat ctcgctgatc gctcgatgca cggcgtcggc gcgcgactct ccgcggagca
     2041 tgtgcgtgta gatgttctcg acgacgacga tggcgtcgtc gatgacgagt ccgatcgccg
     2101 ccgccacgcc gcccagcgtc atcaaatcga aactcaggcc gacgatccac atcgccacga
     2161 aggtcacgag cacggtgacc ggaatgacga gcgtggccac gagcgtcgtc ccccagttgc
     2221 gcagaaagcc gtagagaatc gcgacggaca gaatgaggcc gatgaggatg gcgtcgcgca
     2281 ccgacgacac cgatccgctg acgagcagcg actgatcgta gtaaggcttg atctcgacgt
     2341 cgcgcggaag ttgtttgcga agagcggcca gctccgcctt cacgccgtcg gccacggaca
     2401 gaatgttcgc ggtgggctgc cgcagcacgt tcagcagcac ggccgggcgt ccgtctgccg
     2461 tcacgatcgt gaatcgcggc tcgagtccgg ggctcacggt ggcgacgtcg ccgacacgca
     2521 ccggcacgtt gttgaccgtc gtcacgacga tcgcggcaag gtcatcgagg gaagtggcct
     2581 gtccgctgac gagtgccagc tccaattgat gattctcgtt gatgaggccg ggcgaattga
     2641 tcacgttcga gccgcgcact gcatccgaaa cctgttgcag cgaaacgccg cgcgcttcga
     2701 gccgtgagcg gtcgatgagc acgtggtact cgcgcaactg cccgccaagc acctccacgc
     2761 tggccacgcc gtcgatccgc gcgatcggcg gacggag
//
LOCUS       DATLVW010000813         3711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404276, whole genome shotgun sequence.
ACCESSION   DATLVW010000813 DATLVW010000000
VERSION     DATLVW010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404276"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNN25_23815"
     CDS             complement(<1..524)
                     /locus_tag="VNN25_23815"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HXH94624.1"
                     /translation="MAKNRVVLRGSERPPIPNAENPRQADRHERFTVSVVLRRRSELT
                     PPAGDFLSREHLVTVHGATASDLSVVRDFAREFGLQVLAESSAKRTIQLAGTVEAMEA
                     AFGVLLETVEVNGKTHRQRVGPIHLPEDVAPVVRAVLGLDNRQQADTRSRFAATGSFR
                     PFAAGGGALSPLDVA"
     gene            complement(1332..1685)
                     /locus_tag="VNN25_23820"
     CDS             complement(1332..1685)
                     /locus_tag="VNN25_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94625.1"
                     /translation="MTGFDMLQDWARIGNIRNDDEVWSPVAQVWRPAREVPDLAIADV
                     STPQASATMSFNVRGRQMQVIPVVVVDLEMPFASMVGFIVKWALASIPAPLIILFLAF
                     TFAGVLTSVFRGIGK"
     gene            complement(2184..2528)
                     /locus_tag="VNN25_23825"
     CDS             complement(2184..2528)
                     /locus_tag="VNN25_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94626.1"
                     /translation="MPDHKHLFVSAQGAGYIIDLKTKTLVEEIGLDVARVLVDEQGTL
                     FIVDHNGWSLEGFGKRSRLWKTGPIGCGGIRDTALTNTRLIGETRQPFPPWWVGFSVE
                     LATGEVRFPDAL"
     gene            2994..3269
                     /locus_tag="VNN25_23830"
     CDS             2994..3269
                     /locus_tag="VNN25_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXH94627.1"
                     /translation="MTRKRKTKRWPVQDAKARFSELLDASLVSGPQIVTRRGEEMAVL
                     VRVDHWRQLEEATRPRLKDLLLAAAPRAEVPVTPRRAQRRREPPAFE"
     gene            3276..3392
                     /locus_tag="VNN25_23835"
                     /pseudo
     CDS             3276..3392
                     /locus_tag="VNN25_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
BASE COUNT          723 a         1143 c         1078 g          767 t
ORIGIN      
        1 gcgacgtcga gaggcgaaag ggcaccacct cccgcggcga atggacggaa gcttccggtc
       61 gccgcgaagc gcgaacgagt gtctgcctgc tggcgattgt ccagccccag aaccgcgcgc
      121 acgacgggag cgacatcctc cggcagatga atcggtccga cgcgctgacg atgagtcttt
      181 ccgttcactt cgactgtctc cagcaggacg ccgaacgccg cctccattgc ctcgacagtg
      241 ccagcgagtt gaatagtccg cttcgcggag ctttcggcca gcacctgcag cccgaattct
      301 cgagcaaaat ctcggacaac cgacaggtcg cttgccgtag ccccgtgtac cgttacgaga
      361 tgctcgcgac tcagaaaatc tccagctgga ggtgttagct cactgcgtcg ccgcaggaca
      421 acggatacag taaacctctc atggcggtct gcctgccgcg gattctctgc attcggaatt
      481 ggtgggcgtt cgcttccccg aagaacaacg cgattcttgg ccatgtgctt tgcctctcgt
      541 attcgttaga cggagactgg ccgttttttc cggggaagcg acccgcacgt tgcgcggtca
      601 tgattccaac tcgcacgttc atctctgccc cataggaact ctcataaccg catccctcag
      661 gcggacgctt gtccgcgccg tgttctttgt tgcccggcca gggcggatcg cagaaagctt
      721 gattaaggcg caagacttga cactcagccc cagttttcta agcccctccc agatacgacc
      781 ctgcttgccg ccactcgcgt cgcatcagtg gtgaccgtag caatccggcg acggtcggta
      841 gcctttggag attgccgccg ttcgttgcat cggcttggcc gtcgctggtg ccgcgcaatt
      901 cgaccggtag taaaacttcg tgagtgcatc cccgagaact gttacgcgcg ggattgttga
      961 tggcgcccct tgagaggcct gtttcttcgt cgctgtagac gccgcctgag cgggccaccg
     1021 gagatgaacg tctgtcagcg ccggctctga aggtgggacc ggccgattcg catcaaggtc
     1081 ggtttcgtga agggacgatg acgcgcccga gcccggcgcc gatcgcacgt aagcatggac
     1141 gtcggttccg tcctttcgcg tgtagctgtg tacgtagacg ctgctaccat gcgacccggg
     1201 agacccgccg cttttgccgc caccgcctcg tccggatgca aaggtcgaga aggagaggac
     1261 gaacatcacg atgacgattg cgcttcggtg cattacgttc attggttctt ccttggctct
     1321 cccgggccgg tctatttccc aatgcctctg aacacgctcg tgagaactcc tgcaaacgtg
     1381 aacgccagga agagaatgat caatggagcc ggaatcgatg cgagcgccca cttgactatg
     1441 aagccaacca ttgacgcaaa cggcatttca agatcaacaa ctacgacggg gattacttgc
     1501 atctgcctgc cacgaacatt gaagctcatc gtggccgagg cttgcggtgt cgatacgtcg
     1561 gctatcgcta ggtcgggcac ctcgcgcgcc ggacgccata cctgcgcaac cggactccag
     1621 acctcgtcgt cgttgcgaat gttgccgatt cgagcccaat cctgaagcat gtcaaaaccg
     1681 gtcatacgcc attctttatt acctcgtttc aggagccaat gatctgactt catatgattc
     1741 ctttcccgaa tgtgaccgcg gggctgctgc ttgattcccg atcaaggaac acaggttccg
     1801 gctcgtcgtg tgcggcgatg caaggcaccc gttccgggca tcatgtatat cgacaggtag
     1861 aaagacacgg ttccgatcta agagtggcga atcgaggagc agccgcggca gcgcggaggt
     1921 cacgggaatc acgattgggc cacgcctccg ctcgttgatt cgtgttcctt cgctaaacgg
     1981 ccgcggattg tcatgatgtc ggtgattctt tcgcgtgaag cgctttggac tgcggcagcg
     2041 ccagctgccg ctttcgaaca cgtgattcaa agcggtggct gcgccaccgc actccacagc
     2101 gacttcgtcg cgaaagaaag gcaacggaat cgactggagc tatgacaaat caactccgtc
     2161 cgccactccg tccctcccta tcttcaaagc gcatccggaa aacgaacttc gcccgtcgcg
     2221 agctccacgg aaaaaccaac ccaccagggc gggaatggct gccgcgtttc gccgatgagc
     2281 cgggtatttg tgagcgctgt gtctcgaatt cctccgcaac cgatggggcc cgtcttccaa
     2341 aggcggctgc gctttccgaa accttccagg ctccagccgt tgtgatcgac gatgaacaac
     2401 gtcccttgct catccaccaa cactctggcg acgtccaggc cgatctcctc cacgagcgtt
     2461 ttcgtcttca ggtcgatgat gtacccggcg ccttgcgcgg atacgaacag gtgcttgtgg
     2521 tcgggcatca tggagatcgt agtgacgctc atgtgaccgg tctcgaagct tccgatccac
     2581 gacccttcgc cgatcccgat gcgagaaaaa tgcatccccg tccggagtat ctgtcttccg
     2641 gcgggaagtc gatgaccgct tcgtccgagg acggtggagt gtcgaggacc tcggccccgg
     2701 atgcctgata gcgcgcttcg accgtgggat cccgggccaa cgaaaccttg tagggaaacg
     2761 gagcgtgcgg cttgcgtcga tgatcgtgcg ccagcttgcc gaaccacccc aggaaacgaa
     2821 aaagctggta tcggagtacg gcctcactgc cgggaccttc ttcttccgcc gaccgcgccg
     2881 tctccttgat caacctcccg accgtttctt tgaccgcggg tggtttccct cgaagacgcg
     2941 tcgagccata atgagtgctg actagtcaga tgactagctc aggagacgat tcggtgactc
     3001 gaaagagaaa gacgaaacgt tggccggtgc aggacgccaa agcacggttc agtgaattgc
     3061 tcgatgccag cctcgttagt ggcccgcaga ttgtgactcg gcgcggagag gagatggctg
     3121 tcctcgtccg cgtcgatcac tggcgccaac tggaagaggc cacacggcca cggctgaagg
     3181 acttgttact ggccgccgca ccgcgagcgg aggtgccggt aactccgcgc cgggcgcagc
     3241 gccggcggga gcctcctgcg ttcgagtgag ctatcttgta ccttctcgat acgaatgtgg
     3301 tgtccgaatt gcgcaagccg cggcctcacg gcgccgtggt ggcgtggctc ggcggaacgg
     3361 cggacgagga ccttcacctc tccgcggtca cttcactgtg ttccaggttg acaccctcga
     3421 cccgttcgca acgaagtgag cggtgagaag cgccgggata cctctctgga ggtcatcaat
     3481 tcgatcgcga tcagtcccac cactccaatc acgaccggtc gtccagagga accacgatta
     3541 gggccacgcc ttcgctcgtt gattcgtgtt ccttcggtaa gcggccgcga attgtcatga
     3601 tgtaggtgat tctttcgcgc gaagcgcttt ggactgcggc agcgccagct gccgctttcg
     3661 aacacgtgat tcaaagcggt ggctgcgcca ccgcactcca cagcgacttc g
//
LOCUS       DATLVW010000814         4087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_612137, whole genome shotgun sequence.
ACCESSION   DATLVW010000814 DATLVW010000000
VERSION     DATLVW010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612137"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VNN25_23840"
     CDS             <1..712
                     /locus_tag="VNN25_23840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXH94628.1"
                     /translation="NDLTSFRCLYRKRSFCIMAEVTLEVTRRERSGKEYAKKLRQDGK
                     IPAVVYGGHKEPVAITVERKAVSDLIQKSDHGIRSIFLLKMSGTDQQRHAMIKDMQMD
                     PISRRMTHIDFVRVVMDEVVRITVPVNITGNSIGVKEGGILDWQIRELHVECLPSAIP
                     DKIDVDITNLGAHEQVRVSDLQLPEGVKVSEDVHRVIVGVTSPRAEIVAEVAAVAEPV
                     AGEPEVIKKGKPETETKE"
     gene            785..1027
                     /locus_tag="VNN25_23845"
     CDS             785..1027
                     /locus_tag="VNN25_23845"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXH94629.1"
                     /translation="MSRITRLTKDQVTDDVREKFRQIGAARGNVPNMFRVYAHRPEIL
                     KTMMAHMDAVTNSGTVSVRTKELVATLVSRLNRCEY"
     gene            1079..1324
                     /locus_tag="VNN25_23850"
     CDS             1079..1324
                     /locus_tag="VNN25_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94630.1"
                     /translation="MQALLDFESGPFDDKEKAALAYAKQLTIDAHGVDDALFERLRQH
                     YDEGEIVEISAMAGLFNYFNRVNDALQMEPTKPGEGL"
     gene            1321..2469
                     /locus_tag="VNN25_23855"
     CDS             1321..2469
                     /locus_tag="VNN25_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94631.1"
                     /translation="MRSLIALSVLLLLSVAPLPPSTAVAPVAFPFGHTTSAPYVVADG
                     SGGFDVSWFDRHTKSFNFARYDGAKWSKTTVVAHGRVLDNRADYPSIAVSGRAVIAQW
                     REGVGEEGAQILLARSSDGGATWSKPVTPHPVMEREFGFVSILPLANGTARIAWLDGR
                     ASAHEGSGDTQLRAATMTATGSLTDEVLVDGRVCDCCQTSMVMTSRGPLMAYRDRSEK
                     EIRDIVVAPPQASAHSTPVHADGWLIKGCPVNGPRIDARGNNVAVAWFTAANGNQVVN
                     VAFSHDGGVTFGAPIRAGAEHDAGRVDLVLLGDGTSAMVTWVERAGVFSKVMARRVSA
                     SGVLGKPEVLGKGMTLGFPRIAIANEHILAAWSGDDGIQLATMDTRIR"
     gene            2478..3104
                     /locus_tag="VNN25_23860"
     CDS             2478..3104
                     /locus_tag="VNN25_23860"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH94632.1"
                     /translation="MKRSLQSTVTLLTTLCLAGMVACAKQETPVTGTTQSAAKSKTPG
                     AKPQTTASTTGAEPESARTEVGDPMPTYAAAYLDGKPLDLAAEKGNVVFLNVWATWCG
                     PCRFETPELQALQNQYAASGLKVIGVSVDEGDAAAVKTFVAEQKITYPIAVDPEGRIA
                     TLLQTTVLPTSLLIGRDGKIVWRQIGAVMPGDAKLKAAVEKAVAAKRG"
     gene            complement(3189..3374)
                     /locus_tag="VNN25_23865"
     CDS             complement(3189..3374)
                     /locus_tag="VNN25_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94633.1"
                     /translation="MSSIVILLIAALAVIVAIGLAYVPLSLLVAHMARNLREFIQRQR
                     ERRVVHRETPDRRKVTP"
     gene            3465..>4087
                     /locus_tag="VNN25_23870"
     CDS             3465..>4087
                     /locus_tag="VNN25_23870"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXH94634.1"
                     /translation="MADVPDPPPIPRSLQAPLRALLRGEAAPWPPEASSDDAAKLLEA
                     LDQHGVAPLVYARLSAAGETWPLLATLRGTAIQAFAAETLRLVDVRTLLDAFAARDIR
                     VLIIKGTGLAYDVYDAPELRPRGDTDLLIAEEDREAMRALMHQLGYESQITSGDTLAV
                     RQQTFRRGGHVYDVHWDVTNSPVVRDALPFDELLRRAIAVPRIGAHA"
BASE COUNT          795 a         1264 c         1329 g          699 t
ORIGIN      
        1 caacgactta acatcgtttc gctgtctcta ccgaaaaagg agtttctgca tcatggctga
       61 ggtcacgttg gaagttaccc gtcgcgagcg ctctggaaaa gagtacgcga agaagttgcg
      121 ccaggacggc aagatcccgg ccgtagtcta tggtggtcac aaagagccgg tggcaatcac
      181 cgtcgagcgc aaggctgtct cagacctgat tcagaagagc gatcacggca tccggtccat
      241 cttcctcctc aagatgtccg gcaccgatca gcagcgccac gccatgatca aggacatgca
      301 gatggacccg atctcgcgcc gcatgacgca catcgatttc gtgcgcgtcg tgatggacga
      361 agtggtccgc attaccgttc cggtcaacat caccggtaac tccatcggcg tcaaagaagg
      421 cggcatcctc gactggcaga tccgcgagct tcacgtcgag tgtctcccca gcgccatccc
      481 cgacaagatc gacgtcgaca tcaccaacct cggcgcacac gagcaggttc gcgtttccga
      541 ccttcagctc cccgagggtg tgaaggtgtc cgaagacgtg caccgtgtca tcgtcggcgt
      601 cacgtcgccg cgtgccgaga tcgttgcgga agtcgcagcc gtggccgagc ctgttgccgg
      661 cgagcccgag gtcatcaaga agggcaaacc cgagaccgag accaaagagt aacttcgatc
      721 gtcatcaatt cgcttacgat tacgccccgc ggcaacgcgg ggcgttttgt ttccaggaga
      781 atcgatgtcg cgcatcacca ggctcaccaa agatcaggtc acggacgatg tccgcgagaa
      841 gttcaggcag atcggcgccg cgcgcggcaa cgtgccgaac atgttccgcg tctatgccca
      901 ccggcccgag attctgaaga cgatgatggc gcacatggac gccgtcacga acagcggcac
      961 ggtctccgtc cgcaccaaag agctcgtggc tacgctcgtc tcgcggctca accgatgtga
     1021 atactgaaac cgctcccaca ccgcgcaggc tgcgcggcac ggagcggacg atgcgcagat
     1081 gcaggctctg ctcgatttcg agagcggacc gtttgacgac aaggaaaagg cggcgctggc
     1141 ctacgcgaag cagctcacga tcgacgcgca cggtgtcgac gacgcgctgt tcgagcggct
     1201 gcgccaacat tacgatgaag gggagatcgt cgagatctca gcgatggcag gtttgttcaa
     1261 ctacttcaac cgcgtgaacg acgctctgca gatggagcca accaagccgg gagaagggct
     1321 atgagatcgc tgatcgcgct ctccgttctg ctgctcctca gcgtcgcgcc actgcctcca
     1381 tcgacggctg tggctccggt tgcgtttccg ttcgggcata cgacgtcggc gccttatgtc
     1441 gttgccgacg gcagcggcgg tttcgacgtt tcgtggttcg atcgccacac gaagtcgttc
     1501 aacttcgcgc gctacgatgg cgcgaagtgg tcgaagacta cggttgtcgc tcacggccgc
     1561 gtgttggaca accgcgcgga ctatccatcg atcgctgtct ccggacgcgc ggtcattgcg
     1621 cagtggcgcg aaggggtggg tgaggagggg gcgcagattc ttctggcccg atcgtccgac
     1681 ggcggcgcga catggagcaa gccggtgacg ccgcatccgg tgatggagcg cgagttcggt
     1741 ttcgtatcga tcctccctct cgcaaacgga accgcgcgca tcgcatggct cgacggccgc
     1801 gccagcgcgc acgaaggctc gggagacacg cagctgcgcg ccgcaacgat gacggcgacg
     1861 ggatcgctga cggatgaagt gctcgtcgac ggacgcgtct gcgattgttg ccagacatcg
     1921 atggtgatga cgtcgcgcgg gccgctgatg gcttaccgcg accgatcgga gaaagagatc
     1981 cgcgacatcg tcgttgcccc gccgcaagcg agcgcgcata gcacgccggt ccatgccgac
     2041 ggctggctga tcaaagggtg tccggtgaac ggtccgcgca tcgacgcgcg cggcaacaac
     2101 gtggcggtgg cgtggttcac tgcggcgaac ggcaaccagg tcgtcaatgt cgcgttctcg
     2161 cacgatggcg gtgtgacgtt cggagcgcca atccgcgccg gcgcggagca cgacgcaggc
     2221 cgcgtcgacc tcgtcctcct cggcgacggc acctccgcga tggtgacctg ggtcgagcgg
     2281 gccggcgtgt tctcgaaggt catggcccgc cgcgtcagcg cttcgggagt gctgggcaag
     2341 ccggaagtcc tcggcaaggg catgaccctc gggtttcccc gcatcgcaat cgcaaacgaa
     2401 cacattctcg ccgcatggag cggcgacgac ggaattcaac tagccacgat ggacactcgc
     2461 atacgatgaa catgatgatg aaacgatccc tgcaatcgac agtaaccctg ctgaccacgc
     2521 tctgcctcgc cggcatggtg gcgtgtgcga aacaggagac ccccgttacg ggaaccacgc
     2581 agtccgcggc aaagtcgaag acacccggcg cgaagccgca gacgaccgca agcaccacag
     2641 gcgccgagcc ggagtcggcg cgcaccgaag tcggcgatcc gatgcctacg tacgcggcgg
     2701 cctacctcga cggcaagccg ctcgacctcg ccgccgagaa gggcaacgtc gtctttctca
     2761 acgtatgggc gacgtggtgc gggccctgcc gcttcgagac gccggagttg caggcgctgc
     2821 agaatcagta cgcagcaagc ggcctgaagg tgatcggcgt cagcgtcgac gagggtgacg
     2881 cggccgcggt gaagacgttc gtcgccgaac agaagatcac gtacccgatc gctgtcgatc
     2941 ccgagggacg catcgccacg ctgctgcaga cgacggtgct accgacatcg ctgctcatcg
     3001 gccgcgacgg caagatcgtc tggcgccaga tcggcgcggt catgccgggc gacgccaagc
     3061 tgaaagcggc tgtcgagaag gcggtcgctg ccaagcgcgg gtaacgacaa aaacaaacca
     3121 cagagacaca gaggtcacag aggtgaaggt gttctctgtg acctctgtgt ctctgtggtg
     3181 gcgagccttt atggcgtgac tttgcggcga tcgggcgttt cgcgatgaac cacgcgccgt
     3241 tcgcgctggc gctggatgaa ttcgcgcagg tttcgggcca tgtgtgcgac gagcagactc
     3301 aggggaacgt acgcgaggcc gatggcgacg atgactgcaa gggcggcaat gagaagaatg
     3361 acgatcgagg acatacctgg atacttcaag tagcaaactc caggccgcac tcgcgagcat
     3421 cgtgtgaact attgcggcgg cagccgaggt tggcagtcgc accgatggcc gacgttcccg
     3481 atccgccgcc gattccgcga tcccttcagg caccgctgcg cgcactgctg cgcggcgagg
     3541 ctgcgccctg gccacccgag gcatcgagcg acgacgcagc gaaattgttg gaagcgctcg
     3601 atcagcacgg cgtcgcgccg ctggtttacg cgcggctttc cgcggcaggg gagacatggc
     3661 cacttctcgc aacgctgcgc gggacagcga ttcaggcctt cgcggcggag acgctgcggc
     3721 tggtcgatgt tcgcacgctt ctcgatgcct ttgctgcccg agatattcgt gtcctgatca
     3781 tcaagggaac cggtctcgcg tacgacgtgt atgacgcgcc ggagctgcgt ccccgagggg
     3841 ataccgacct tctgattgcg gaagaggatc gcgaggcgat gcgtgcgctg atgcaccagc
     3901 tcggctacga atcgcagatc acgagcggtg acacgctggc ggtgcggcag cagacgttcc
     3961 gccgcggtgg tcatgtgtat gacgtgcact gggatgtcac gaattcgccg gtggtgcgcg
     4021 acgcgttgcc gttcgacgag ctccttcgcc gcgcgattgc cgtgcctcgc atcggcgcgc
     4081 atgcgtg
//
LOCUS       DATLVW010000815         3700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_612174, whole genome shotgun sequence.
ACCESSION   DATLVW010000815 DATLVW010000000
VERSION     DATLVW010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612174"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VNN25_23875"
     CDS             complement(<1..231)
                     /locus_tag="VNN25_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXH94635.1"
                     /translation="MIHASSAQAFAIARYRAMHESGCFVLPNPWDVGTALYLQHLGFE
                     ALATTSAGVAFTRGLPDTVAAIPRDLMLEHIRD"
     gene            complement(256..666)
                     /locus_tag="VNN25_23880"
     CDS             complement(256..666)
                     /locus_tag="VNN25_23880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH94636.1"
                     /translation="MSTNDNSDVPALFRINVEVGDLDAAVAFYAALFNVQGRKLPGSR
                     CYFQCGPVTLSVLDVSSVGTPHPAAKALYFTVRNLDAAFERAKSLGCLSRENVHDAPG
                     GGIIVRPWGERSFYAEDPWKNPLCFVDEGTVYRG"
     gene            complement(761..>3700)
                     /locus_tag="VNN25_23885"
     CDS             complement(761..>3700)
                     /locus_tag="VNN25_23885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH94637.1"
                     /translation="DDSRRAVRAALALIETLGKLSEKMESDLGVRLAVRAGVHTGIIL
                     AETGRDTVDARVADSDTVITAARVRELAPLNSVVVSEATRELVADYFETTESESVTVG
                     SSRQVWQTYLIVRDRGFQSRVEASAATKRLSELVGREQELDLLADHWQSAQNGDGQVV
                     MLRAEAGFGKSRLIAELKSRTAAQPRHWIECRCSPYHQNSALYPVVGVFQKWLGLETN
                     EPAEVKLAKVESNLARYGGDLEQSVPLIASLLSIPFESKYKPAALNSREQKQVTLEVM
                     AQIVIDQAMEKPVAFVVEDLHWVDPSTMELLDILVEQAATLPILVLFSFRPEQVAPPQ
                     WLNRGYTSQIALDRLDCDAVRRMTLVLTGGKALPSEVFDEIFAKTEGVPLFVEDLTRM
                     VIESGMLEERDGEYALVGPFQSLAIPATLQETLMARLAKLATAKPVAQVGATIGREFV
                     FEMLRDVGGFDDKPLLEELNKLVSAGLLHRRGLLSHAKYIFKHALVQEALQQSLMKKQ
                     RRHYHKVIAEILEEKFHAVAEGQPELVAYHYGEAAIHAKAVEYWRRAAERALASCANR
                     EALSHARHAIETLQHLPESDESHEAELALRAIEGPALLALKGWAAPELADCYQRAREV
                     CATLPRTPKLFSVLRGIWTNEMVSAHLRQALAVAEELFVMASAADNEDLLLEARGALC
                     DTLFWLGRPAESREQARLGFELYDLDRHHTPHSVAYGEDPATMFYTYSALSLALMGQP
                     RRSLEFTHAAIDTLSRFSHTHSRAFLICGIAWNYIQMRDIEATGRYAQMLIDISVENH
                     FDAWLPIGQAMKGWSIAASGNLEEGAALMKSGRERWHATGAGVKACFYPALLADLHAR
                     AGALDESAEWVAIGFEAAAACDDRYYFSELHRVKGDILAARGQAADAFTSYDEALSIA
                     REQRATLLELRAATVKARLHIACGEADAATSLLAPFLATFDSSLVLPDFIEAAGVNAA
                     ASIGASA"
BASE COUNT          677 a         1179 c         1157 g          687 t
ORIGIN      
        1 gtcgcgaatg tgctcgagca tcagatcgcg cgggatggcc gccacagtat ccggcaatcc
       61 tcgggtgaac gcgacgccgg ccgatgtcgt tgcaagggcc tcgaacccga ggtgctggag
      121 atagagcgcg gttccgacgt cccacggatt cggcaggacg aagcaacccg actcgtgcat
      181 cgcacggtac cgggcaatgg cgaaggcttg tgcggagctt gcgtggatca tgatcgaaac
      241 gctacaccaa caggcctacc cgcggtacac ggttccttcg tcgacgaaac agagagggtt
      301 cttccacggg tcctcggcgt agaaggagcg ctcgccccaa ggccggacga tgatcccgcc
      361 gccgggcgca tcgtgaacat tctcgcggga taggcagccg agggacttgg cgcgctcgaa
      421 tgctgcgtcg agattcctga ccgtgaagta gagcgccttg gcagcgggat gaggcgttcc
      481 caccgatgac acatcgagca cagagagcgt caccgggccg cactggaagt agcagcgtga
      541 tcccggaagt tttcgtccct gcacgttgaa caatgctgcg taaaacgcga cggcagcgtc
      601 gagatcgcct acttcgacgt tgattcggaa cagcgcgggt acgtccgagt tgtcgtttgt
      661 ggacattatt cgatcctcct cgcggaccac agagatgaac acgtactctg cgacctctcc
      721 gtctctgtgg tcaaccagat tcgttacatg tgacgggcga tcatgcgctc gcgcctatgc
      781 ttgctgcggc attgacacct gcggcttcga tgaagtctgg gaggacgagg ctactgtcaa
      841 acgtggccag gaacggcgcc agtagggagg tggcggcatc ggcctcaccg caggcgatgt
      901 gcaatcgcgc tttgacggtc gctgcgcgca gctcgagcag cgtggccctc tgttctcgtg
      961 cgatcgataa ggcctcgtcg taggaagtga aggcatcggc ggcttggccg cgcgcggcca
     1021 ggatgtcgcc ttttactcga tggagctccg agaaatagta gcggtcgtcg catgccgcag
     1081 cggcctcgaa gccaatggct acccattcgg cgctctcgtc gagcgcgccc gcacgcgcgt
     1141 gaagatccgc caggagcgcg gggtagaaac aggcctttac cccagcgccg gtggcgtgcc
     1201 atcgctccct cccactcttc atcagcgcgg cgccttcctc cagattgccg gacgcagcga
     1261 tcgaccatcc cttcattgcc tgaccaatgg gtaaccaggc gtcgaagtga ttctcgaccg
     1321 aaatgtcgat cagcatctgc gcgtaccgac ctgttgcctc gatgtcgcgc atctgaatgt
     1381 aattccacgc gatgccgcag atgagaaagg cgcggctgtg cgtgtgcgag aagcggctca
     1441 gagtgtcgat cgcggcgtgg gtgaattcca gcgaacggcg tggctggccc atgagagcga
     1501 ggctcagcgc cgaataggtg taaaacatcg tggctggatc ttcgccgtac gcgaccgaat
     1561 gcggcgtgtg atggcggtcg aggtcgtaaa gctcgaagcc gagcctggcc tgctcccgcg
     1621 actccgccgg acggccgagc cagaagaggg tgtcgcagag cgcgccccgg gcctcgagca
     1681 gaagatcctc attgtccgcc gccgaagcca ttacgaagag ctcctcagcc acagcgagcg
     1741 cttgacggag atgtgccgac accatttcgt tcgtccaaat gcctctaaga acgctgaaga
     1801 gcttcggcgt gcggggcagc gtggcgcaga cctcgcgagc gcgttggtag cagtccgcca
     1861 gctccggcgc cgcccagccc ttcaacgcca ggagcgcagg tccctcgatc gcgcgcagcg
     1921 ccagttccgc ctcatgagac tcgtcgctct ccggcaagtg ttgtagcgtc tcgatcgcgt
     1981 gccgtgcgtg actgagagcc tcgcggtttg cgcatgatgc aagcgcgcgc tccgcggcgc
     2041 gacgccagta ttcgacagcc ttcgcgtgta tcgccgcctc accatagtga taggcgacga
     2101 gctcgggctg cccctcggcg actgcatgga acttctcctc gagaatctcg gcgatcacct
     2161 tgtggtaatg gcggcgctgc ttcttcatca gcgattgctg cagcgcctcc tgcaccagtg
     2221 cgtgcttgaa gatgtacttg gcgtgactca gcaggccgcg ccggtgcagc aggccggcag
     2281 agaccagctt gttcaactct tcgagcagcg gcttgtcatc gaagccgcca acgtcgcgca
     2341 gcatctcgaa gacgaattcg cgcccgatcg tggcgcccac ctgcgccaca ggcttggccg
     2401 tcgccagctt cgccaaacgc gccatcagcg tctcctgcaa agtggcggga atggccagcg
     2461 actggaacgg ccccaccagc gcgtattcgc catcccgctc ctcgagcatg ccggattcga
     2521 tgaccatcct ggtgaggtcc tcgacgaaca gcggcactcc ctcggtcttg gcgaagatct
     2581 cgtcaaagac ctcggacgga agcgctttgc ctccggtgag gacgagcgtc atccggcgca
     2641 ccgcatcgca gtcgagacgg tcgagcgcga tctggctggt gtacccccgg ttcagccatt
     2701 gcggcggagc gacctgctcg ggacggaagc tgaacaggac caggatcggc agcgtcgccg
     2761 cctgctccac gagtatgtcc agtaactcca tcgtcgaagg atcgacccag tggaggtctt
     2821 ccacgacgaa cgccaccggt ttttccatcg cctgatcgat cacgatctgg gccatgacct
     2881 cgagcgtgac ctgcttctgc tcgcgcgagt tcagagcggc cggtttgtat ttcgattcga
     2941 atgggatcga gagcagcgag gcgatcaacg gtacgctctg ttcgagatcg ccgccgtagc
     3001 gtgccagatt cgactccacc ttggccaact tcacctcggc tggctcattc gtctccaggc
     3061 cgagccattt ctggaagacg ccaacgaccg gatagagcgc gctgttctga tgatagggag
     3121 agcagcggca ctcaatccaa tggcgcggtt gggctgcggt ccgtgacttg agctccgcga
     3181 tcaacctcga ctttccaaat ccagcctcgg cgcgcagcat gacgacctgg ccgtcgccgt
     3241 tctgcgccga ctgccagtgg tcagccagaa gatccagctc ctgttcgcgg cccaccagct
     3301 ccgacaagcg cttcgtggcg gccgaggcct ccacgcggct ctgaaatcct ctgtcgcgca
     3361 cgatgagata ggtctgccag acctgccggg acgatccgac cgtgacgctc tcgctttccg
     3421 tggtttcgaa gtagtccgcg accagttcgc gtgtggcctc gctgacgacg acggagttga
     3481 gcggtgccag ctcacgcacg cgagcggctg tgatcaccgt gtcgctatcg gccacgcggg
     3541 cgtcgacggt gtcgcgtccc gtttcggcaa ggatgattcc ggtatgtacg ccggcgcgca
     3601 cggccagacg cacgccgagg tcgctttcca tcttctcgct cagcttgccc agcgtctcaa
     3661 tcaatgccag agccgcacgc acggcccggc gcgaatcgtc
//
LOCUS       DATLVW010000816         8961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_283010, whole genome shotgun sequence.
ACCESSION   DATLVW010000816 DATLVW010000000
VERSION     DATLVW010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283010"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..502
                     /locus_tag="VNN25_23890"
     CDS             215..502
                     /locus_tag="VNN25_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXH94638.1"
                     /translation="MPALIIVDIEVLDPARYEDYKRLASATIAAHGGRYLVRGGKSEV
                     LDGEWQPRRLVVLEFDSMEKAKAWRDSPEYAEAKKVRDECARASMIVVEGV"
     gene            581..1642
                     /locus_tag="VNN25_23895"
     CDS             581..1642
                     /locus_tag="VNN25_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94639.1"
                     /translation="MKNRCAAALVLAVSLFVVPVFAQSTTGEKPATATATPTTTSAAT
                     STAPEQKAPDEKAAAEKGPIRVEVGTYVNQILALDLKGNQFTADFYVWFRWKGDAKVF
                     DTFDLANGRITSRTGIVKKDLDGGVHYASARILATITKFWDLRRYPLDDHQLTIEIED
                     NENEDNLLVYVPDKTNSSASPTVEVPGWRVAGTSVSVTSHKYATNYGDTSVPTGNASS
                     YSRFVSAVDIKRPGHIGRFFKVFFTLFIATIISWLAFWVRPKETGPRISLCVGSVFAA
                     AAATFAINNMLPDTNSTTMSDRLIVVSLSCIFIATLQTIVAINLGYAGKESAQRLLDR
                     VCAWLFPLLYVVLLVTYVI"
     gene            1805..2065
                     /locus_tag="VNN25_23900"
     CDS             1805..2065
                     /locus_tag="VNN25_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94640.1"
                     /translation="MTQSQKSVRQSVSLPSQVARRVQALAKRSRTSANRVIVDLIESG
                     LEAKEREKADFFDIADRLARTSDPAEQKSLKEELARMTFGEP"
     gene            2074..2316
                     /locus_tag="VNN25_23905"
     CDS             2074..2316
                     /locus_tag="VNN25_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94641.1"
                     /translation="MPRIQWTNLPPALRDHLFERLRDRKITVEDLYQLKLWRESEPEA
                     PDGQWVKDFGSFKICGEGRYPKTFLLRGQTAIGQKL"
     gene            complement(2419..2967)
                     /locus_tag="VNN25_23910"
     CDS             complement(2419..2967)
                     /locus_tag="VNN25_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HXH94642.1"
                     /translation="MKRIVLIFGLISGVISSALMFLTLPLLHSGKVTFDNGEIIGYTA
                     IFLSLLLVFFGIRSYRENAGGTITFGRAFSVGILITLISCVFYVASWEVIYFKFMPNF
                     ADKYSAHVISEMRAKGATDADIAAKKVEMVKMKAMLDNPLLNAAMTFIESFPVGLIMT
                     LVSAGILRRRRAPPALAVAQAI"
     gene            complement(3058..3510)
                     /locus_tag="VNN25_23915"
     CDS             complement(3058..3510)
                     /locus_tag="VNN25_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXH94643.1"
                     /translation="MIKQVALYGAIGGVLIALLKVIEYKHFVQEYPGEIYGGLVALVF
                     TALGIYFGLRWTRAKQVVVIREVPVEVRTDGPFVLNAEKLKEIGLTAREHEILAHIAQ
                     GLSNREIGEKLFVSENTVKTHSSRLFEKMQVNRRVQAVQKGKEFGLIP"
     gene            complement(3524..4075)
                     /locus_tag="VNN25_23920"
     CDS             complement(3524..4075)
                     /locus_tag="VNN25_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94644.1"
                     /translation="MIDEALRKRLVAFLQPLYQDLDGVSRFEEIERVAAIARRLYTPA
                     SNDARAFELLLLFHGLGKWIEKIGNRSRAVLVTGLSDDELRALAGSIRRLDAPSSDAE
                     RALAAAIVIDSAGVRGLAERLSRARREGSSVMDVVRDALADAIVPEWMPEAGRKWLLA
                     RYEARREMCRGILREMSLEDLDL"
     gene            complement(4072..5367)
                     /locus_tag="VNN25_23925"
     CDS             complement(4072..5367)
                     /locus_tag="VNN25_23925"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH94645.1"
                     /translation="MPGLTDESPQARRIAAIALGKRGGPGAVAALRAALAREDVLWVR
                     SSMVLALGKIGDDDARAALAEVEPRSEAESEALRKALDRVSAPATGFAWRSDVTNDRL
                     FASVPLGLEDVAIAEAAERKIEASLVRRGVIALRAPMPGLRNALRCAYDVRFLVAEGA
                     AGAFAETIARAQVPWREWIHSESNALPYRFSLENVRVTRQQFSDVLRLARETFAPLGL
                     IDSPSNYAAMLRVEADADATRIWFVPTFEPDERFAYRIADVGASINPVVGACLARLVR
                     GKASRVVVDPTCGSGTLLIERALLDSDVLLSGIDISPTAIRVATGNVIAAKLAARIAI
                     RQADGGDSAAWPDRCDEVLANFPFGNRTEDRNLDGLYRRVVRNIGRTLAPGGRALLYT
                     AHTKTLIALLRRTPQLAVREERRVESGGLQVGLWLLEKR"
     gene            5503..6927
                     /locus_tag="VNN25_23930"
     CDS             5503..6927
                     /locus_tag="VNN25_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXH94646.1"
                     /translation="MTEFDAVVVGSGPNGLSAAIVLAREGLHVLVLEAHESIGGGTRT
                     EELTLPGFRHDVCSAIHPMALVSPFFPTLPLKDHGLEWRYSPYAISHPLDDGSAAVME
                     LSIEKTAATLGEDGDSYRRLMAPLAGNAEELFAEILRPMPIVPRHPLLMARFGLAGLR
                     SALAIANRFRSERARALFGGCGAHSFLPLEAAGSASFGLVLAVAGHAVGWPCAKGGSS
                     AITDALSSYFCSLGGTIRTSSPVRTMSDLPKSRVVLFDVTPRQLAAIAGDDLPPSYAK
                     RLRRFRYGPGVFKVDWALDGPIPWRAEECRQAATVHVGGTIEEIADHERTIWQGRSTN
                     RPFVLVAQQSLFDDTRAPAGKHTGWGYCHVPHGSTEDMTEAIEQQIERFAPGFRERIL
                     ARHTINTAAYESYNANFVGGDISGGANNLTQILARPFLSRDPYATPNKGVYLASSSTP
                     PGGGVHGMCGYWAARSALRRAFGR"
     gene            complement(7083..7382)
                     /locus_tag="VNN25_23935"
     CDS             complement(7083..7382)
                     /locus_tag="VNN25_23935"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HXH94647.1"
                     /translation="MQIVFPHHTDRASARRKIEARLEELLGQHGHYLTDITQHWEGDR
                     LVFSGSAKGFKANGSVEVTDSDVIVDGKLPLIARPFESRIKSTIEHEAAAMFPAA"
     gene            7476..>8961
                     /locus_tag="VNN25_23940"
     CDS             7476..>8961
                     /locus_tag="VNN25_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXH94648.1"
                     /translation="MLAKVWSAATLGIDAVRIVVEVDARKADTPGFTLVGLPDAAVRE
                     AYSRVRSALRHCGYWLPQRHFTVNLSPADVKKEGSAFDLPIAIGILVVAEFLPPDLVA
                     GRLFVGELSLDGAIVPVRGTLAISASFAHDEELRELIVPAGNASEAAAVESVRVCGAN
                     HLPELLRHLTGDVVIERAVSAPVFEPVREVPDFSDVRGQLQAKRALEIAAAGGHNILM
                     IGPPGSGKTMLARRLPSILPRLSLDESITTTKIHSVAGTLPAGAGLITVRPFRAPHHT
                     ISVAGLVGGASLPRPGEVSLANHGVLFLDELAEFQRTTLEVLRQPMEDGHVTIARVQR
                     TLTFPSRFTLAAALNPCACGYFNDSRRQCICSPGQIARYLGKISGPLLDRIDLQVEVP
                     ALAVEEISTFTPGENSSSIRDRVEAAREIQRQRFARVSWKSNAEMTSRAMRKYCELDP
                     ASRRILDQALTHLGLSVRAHDRVLKVARTIADLLGSEKIQSAHVA"
BASE COUNT         1663 a         2876 c         2785 g         1637 t
ORIGIN      
        1 ctgccacgtg cattacccgg cactgacgaa gatcatcaag gcgacggctg aggagtacgg
       61 aatgacgtat cgcgagaaca ggaccatcac cggagcattt ctcgcccatc tgcggtggtt
      121 gaaggtgctt ggcaataccg cgtaggacac gtcctcccca ggctgggttc cctgaagctc
      181 aatcggcacc cgccagtcgt gagatcattt ctcaatgccc gcattgatca tcgtcgacat
      241 cgaagtgctc gatcccgccc gctacgagga ctacaagcgc ctcgcttcag ccaccatcgc
      301 ggcgcacggc ggccgttacc tcgtgcgcgg cgggaagagc gaggtgctgg acggcgaatg
      361 gcagccgcgt cgcctcgtcg tcctggagtt tgactcgatg gagaaggcga aggcgtggcg
      421 cgattcgccg gagtatgccg aggcgaagaa ggtgcgcgac gaatgcgcgc gggccagcat
      481 gatcgtcgtc gagggtgtgt agcgcgagcg aattcagcaa ttgacgcggc gagcaggcga
      541 agggtagggt gtcggaactt cgtctggaga ggtcctttgc atgaaaaaca ggtgcgccgc
      601 ggcgctcgtt ctcgcagttt ctctcttcgt tgtcccggtg tttgcccagt ccactaccgg
      661 cgagaagccc gcaacggcga cagcgacacc gacgacaaca tcggcggcga catcaactgc
      721 cccagagcag aaagcgcccg acgagaaggc tgcggccgaa aagggaccga tccgcgtcga
      781 ggtcggaact tacgtcaatc agatcctcgc gctcgatctc aaagggaacc agttcaccgc
      841 cgacttctac gtatggtttc gctggaaggg cgacgccaag gtgttcgaca ccttcgacct
      901 ggccaacggg cggatcacgt cgcgcacagg catcgtgaag aaggatctcg atggcggcgt
      961 gcattacgcg tccgcgcgca ttctcgccac gatcacgaag ttctgggact tgcggcggta
     1021 cccgctcgac gatcaccaat tgacgatcga gatcgaggac aacgagaacg aagacaacct
     1081 gctcgtttat gtgccggaca agaccaacag cagcgccagc ccgacggtcg aagtgcccgg
     1141 ctggcgcgtc gccggaactt cggtctccgt aacttcgcac aagtacgcca ccaactacgg
     1201 cgacacctcg gtgccgaccg gcaatgcgtc gtcgtactcg cgattcgtgt cggcggtgga
     1261 catcaaacgg ccgggacaca tagggcgctt cttcaaggtt ttcttcacgt tgttcatcgc
     1321 cacgatcatc tcgtggctcg cgttctgggt gcggccgaag gaaacaggcc cccgcatcag
     1381 cctctgcgtc ggctcggtat tcgctgccgc cgcagcaacc ttcgccatca acaacatgct
     1441 gcccgacacc aactcgacca cgatgtcgga ccgcctgatc gtcgtgagct tgagctgcat
     1501 cttcatcgca acgttgcaga caatcgtcgc gatcaacctg ggatacgcgg gcaaagaaag
     1561 cgcacagcgt ctgctcgatc gcgtttgcgc ctggctgttc ccgctgctct atgtcgtgtt
     1621 gttggtgacg tatgtgattt agccgcctgt tgaaccacag agacactgag gacacagagt
     1681 tgaacggcat cctctgtgac ctctgtgtct ctgtggtcaa aaggtttcgc taggtcggtt
     1741 gcgccacact agcagcttcg tcgtgtgctg gtgcaagatg gtgcatcccg gtgtaagatg
     1801 agacatgacc cagtcacaga agagcgtccg ccagagtgtc agcctccctt cgcaggtcgc
     1861 acggcgagtc caggcgctcg cgaaacgaag ccgcaccagc gcaaaccgcg tcatcgtcga
     1921 tctgattgaa agcggactcg aggcaaagga gcgcgagaag gctgacttct tcgacattgc
     1981 cgaccggctg gcccgcacct ccgatccagc ggagcagaag agcctcaaag aagaactggc
     2041 acggatgact ttcggcgagc cctaggaatc ccattgcctc ggattcagtg gaccaatctt
     2101 ccgcccgcgt tgcgcgatca tctcttcgag cgcctccgcg accgcaagat caccgtcgaa
     2161 gacctctacc aactcaaact ctggcgagag tcagagccgg aagcaccgga cggccagtgg
     2221 gtcaaagact tcggctcctt caagatctgc ggagaaggga gatacccaaa gaccttccta
     2281 ctccgaggtc agactgcgat tggacagaag ctctgatttc gttgcgggcg atgcctctgg
     2341 acgaggtcga ataaacgcga aaccagccgg cactcgccgc tgaattcttt gagtgccggc
     2401 cggcattgag cgatcggact agatcgcttg agccaccgcc aaggccggcg gcgcgcgccg
     2461 tcgtcgcaga atccccgccg ataccagcgt catgatcagg ccgaccggaa acgattcgat
     2521 gaaggtcatg gcggcgttga gcagcgggtt gtccagcatc gccttcatct tcaccatctc
     2581 gaccttcttc gcggcgatgt cggcgtcggt ggcacctttc gcgcgcattt cgctgatgac
     2641 gtgagcggag tatttatcgg cgaagttcgg catgaacttg aagtagatga cctcccatga
     2701 cgccacgtag aacacgcacg agatcagcgt gatgaggatg ccgacgctga aggcgcggcc
     2761 aaaggtgatc gtgccgccgg cgttctcgcg gtacgagcgg atgccgaaga agaccagcag
     2821 caacgacagg aagatcgccg tgtagccgat gatctcgccg ttgtcgaagg taaccttacc
     2881 gctgtggagg agggggaggg tgagaaacat cagggccgag gagatgacgc cggagatgag
     2941 accgaagatg aggacgattc gtttcatgtg agtttccttt cgcggcgcaa ggtacgtccg
     3001 ggcgaggcat cgcgcagtcg cccgttcagg tgattttcgg gtcaacgaaa gtatgaatca
     3061 ggggatcagg ccgaattctt tgcctttctg cactgcctgg acgcgacgat tgacctgcat
     3121 cttctcgaac agccgcgagg aatgcgtctt cacggtgttc tcggagacga agagcttttc
     3181 gccgatctcg cggttgctga gcccctgtgc gatgtgagcg aggatctcgt gctcgcgcgc
     3241 agtgaggccg atctccttca gtttctcggc gttgagcacg aacggtccgt ccgttcgcac
     3301 ctccaccggc acttcgcgga tgaccacgac ctgcttcgcg cgcgtccatc gcagtccgaa
     3361 gtagatgccg agggcggtga agacgagggc gacgagcccg ccatagatct cgcccggata
     3421 ctcttggacg aagtgcttgt actcgatgac cttcagcagc gcgatgagga cgccgccaat
     3481 ggctccgtag agcgcgactt gtttgatcat ggatggggga agtttacagg tccaaatcct
     3541 ctaaggacat ctcccgaagg attccccggc acatctcccg gcgtgcctcg tatcgcgcca
     3601 acagccactt ccgtcccgcc tccggcatcc actccggcac gatcgcgtca gccagcgcat
     3661 cgcggacgac gtccatcacc gagcttccct cgcggcgcgc acgcgagagc cgttccgcaa
     3721 gtcctcgcac accggcggaa tcgatcacga tcgcggcagc aagagcacgc tcggcatcgc
     3781 tcgacggagc atcgagccgg cgaatggagc cggcgagagc gcgaagctca tcatcggaaa
     3841 ggccggtcac cagcaccgcg cgcgagcggt tgccgatctt ctcgatccat ttgcccaggc
     3901 cgtgaaagag caacagcagt tcgaacgcac gagcatcgtt tgacgccggc gtgtagagcc
     3961 gccgcgcgat ggccgcgacg cgttcgatct cctcgaagcg cgacacaccg tcgaggtctt
     4021 gataaagcgg ctgcagaaat gcgacgagcc gctttcgcag ggcctcgtcg atcatcgctt
     4081 ctccagcaac catagcccga cctgcaatcc gccgctctcg acgcgccgct cttcgcgcac
     4141 ggcaagctgc ggcgtgcggc ggagaagcgc gatcaatgtc ttggtgtgcg cggtgtacag
     4201 cagcgcgcgc ccacctggcg caagcgtgcg cccgatgttg cggacgacgc gtcggtaaag
     4261 tccatcgaga ttgcgatcct cagtgcggtt tccgaacggg aagttcgcca gcacttcatc
     4321 gcagcgatca ggccacgcag cagagtcgcc gccatccgcc tgtcgaatgg caatccgcgc
     4381 ggccagcttc gcggcgatga cgttgccggt cgcgacacgg atcgccgtcg gactgatgtc
     4441 gatgccggag agcagcacat cggaatcgag cagcgcccgt tcgatcaaga gcgtcccact
     4501 gccgcaagtc ggatcgacga cgacgcgcga cgccttgccg cgaacgagcc gcgccaggca
     4561 ggcacccact accggattga tcgaagcgcc gacgtcggcg atccggtacg caaagcgctc
     4621 atcaggctcg aacgtcggca cgaaccagat gcgcgtggca tccgcatccg cttccacgcg
     4681 aagcatcgcc gcgtagttcg agggcgagtc gatgagcccg aggggcgcga aggtctcccg
     4741 cgccaatcgc agcacgtcgc tgaactgctg ccttgtgacg cgcacgttct ccagtgagaa
     4801 tcgatagggg agtgcattcg attcgctgtg gatccattca cgccacggta cttgcgctct
     4861 ggcgatcgtc tctgcaaacg cgccggccgc cccctcggcg actagaaagc gcacgtcgta
     4921 cgcgcagcga agcgcgttgc gaagtcccgg catgggagcg cgcaacgcga tcactcctcg
     4981 ccgcacgagg ctcgcctcga tctttcgttc cgccgcctcg gcgatggcaa cgtcttccag
     5041 tccgagcggc acgcttgcga acagccgatc gttcgtcaca tcgctgcgcc atgcgaagcc
     5101 ggtcgcgggc gcgctcaccc gatcgagcgc cttgcgcaac gcctccgact ccgcctccga
     5161 gcgcggctca acctcggcaa gagccgcgcg cgcgtcgtcg tcaccgatct tgcccaacgc
     5221 caggaccatg gacgatcgga cccacaacac atcctcgcgc gccagcgccg cgcgcaacgc
     5281 cgccaccgca cccggccccc cgcgcttgcc caacgcgatg gcagcgatgc ggcgcgcctg
     5341 cggcgattcg tcagtcagtc ccggcatcgc cgcgcagtgt agttcgctca cgcgaagacg
     5401 cgaagccgct cacgcgaagc cgctcacgcg aagacgcgaa gccgcgaagg agttatgtgg
     5461 ccacttcgcg tcttcgcgcc ttcgcgtgat aagactgctc ccgtgactga attcgatgcc
     5521 gttgtcgtcg gctccgggcc gaacgggctc tcggccgcga tcgtgctggc ccgcgaggga
     5581 ctacacgtcc tcgtgctcga ggcgcacgaa agcatcgggg gcggcacgcg caccgaggag
     5641 ttgacgctgc cggggtttcg gcacgatgtc tgttcggcca ttcatccgat ggcgctcgtc
     5701 tcgcctttct tcccgacgct gccgctgaag gatcatgggc tggagtggcg ttactcgcct
     5761 tacgcgatct cgcatccgct cgacgacgga tcggctgcgg tgatggagct gtcgatcgaa
     5821 aagacggcgg ccacccttgg cgaagatggc gattcgtatc ggcgcctcat ggcaccgctg
     5881 gccgggaacg ccgaggagct ctttgcagag atcctgcgtc cgatgccgat cgtgccgcgg
     5941 catccgctgc tcatggcacg gttcggactg gccggacttc gttccgcgct cgccatcgct
     6001 aaccgatttc gcagcgaacg tgctcgtgct ctctttggcg gatgcggggc gcattcgttt
     6061 ctgccgctcg aagccgccgg ctccgcatcg ttcggactcg tgctggcagt ggccggacac
     6121 gctgtcggct ggccgtgcgc gaaagggggc tcgagcgcca tcaccgatgc gctgtccagc
     6181 tacttctgct ctctcggcgg cacgatccgg acatcgtcgc cggtacgcac gatgagcgat
     6241 cttccgaagt cgcgcgtcgt tctcttcgat gtcacgccgc gccagcttgc tgcgatcgcg
     6301 ggcgatgatc tgccgccgtc gtacgccaag cgtctgcgcc gtttccgcta cggtcctggt
     6361 gtcttcaaag tcgattgggc gctggacggg ccgatcccct ggcgcgccga ggagtgccgg
     6421 caagccgcga cggttcacgt tggcggcacg atcgaggaga tcgccgatca cgagcgaacg
     6481 atctggcagg gacgcagcac caaccggccc ttcgttctcg tcgcgcagca gagtttgttc
     6541 gacgacacgc gcgcgccggc ggggaagcac accggatggg gctactgcca cgtgccacat
     6601 ggctcgaccg aagacatgac cgaggcgatc gagcaacaga tcgagcgctt cgcacccgga
     6661 tttcgcgaac ggatcctggc gcgtcacacg atcaatacgg cagcatacga gtcctacaac
     6721 gcgaacttcg tcggcggcga tatctccggc ggcgcaaaca acctcacgca gatcctggcg
     6781 cgaccgtttc tcagccgcga tccatacgcg acgccgaaca agggagtcta cctcgcctcc
     6841 agctcgacgc cgccgggcgg gggagtgcac ggcatgtgcg gctactgggc cgcacggtcg
     6901 gcgctgcgcc gcgcgttcgg gaggtgagtt tggtacccag accgtccctc gattgctgtc
     6961 gtcgtgagag ccgccgaagc cgctgcaaga tcaaaatacg gagatctcgt tcgagggtct
     7021 tttaatcacg atcgctcgcg ccggacgaac acgatccgcg aagtttgaaa tccttcgacg
     7081 tcctacgccg caggaaacat cgccgcggcc tcgtgctcga tcgtgctctt gatacgcgac
     7141 tcgaacggcc gtgcgatgag tgggagcttg ccgtcgacga tcacatcgga gtcagtgact
     7201 tcgacggatc cattcgcttt gaagcctttt gcggaaccgg aaaagacgag acggtcgcct
     7261 tcccaatgct gggtgatgtc ggtcaggtag tggccgtgct ggccgagaag ctcctccagg
     7321 cgcgcctcga tcttgcgacg tgcgctggct ctgtcggtgt gatgcggaaa gacgatttgc
     7381 atgctgcctc cggcgggcgt tgtatcacac cacttgccat cgctcgctag ccgaggtagc
     7441 ctcggtgcgg gacttccgcg tggtcgcaac ccgccatgct cgccaaagta tggagtgccg
     7501 cgaccctcgg gattgatgca gtacgaattg ttgtcgaagt cgatgctcgc aaagccgaca
     7561 cgccaggttt cacgctggtc ggacttccgg acgccgctgt gcgcgaggcg tattcgcgcg
     7621 tccgttccgc gctgcgccat tgcggctact ggctgccaca gcgccatttc accgtaaacc
     7681 tgtcgcccgc cgatgtgaag aaggaagggt ccgccttcga ccttccgatc gccatcggca
     7741 tcctggtcgt cgccgagttt ctgccgccgg atcttgtcgc cgggcggctg ttcgtcggcg
     7801 agctctcgct cgatggcgcg atcgtgccgg ttcgcgggac gctcgcgatc tcagcgtcgt
     7861 tcgcgcacga cgaggagctg cgcgagctga tcgttccggc cggcaacgct agcgaagctg
     7921 ctgccgtgga gtcggtccgc gtctgcggcg cgaatcatct gccagagttg ctccggcacc
     7981 tcaccggcga cgtcgtcatc gagcgcgccg tttcggcgcc cgtgttcgag ccggtgcgcg
     8041 aggttcccga cttttcggat gttcgaggac aactgcaggc gaagcgcgcc ctcgagatcg
     8101 ccgcggccgg cggtcacaac atcctcatga tcggaccgcc gggatcgggg aaaacgatgc
     8161 tggcgcgacg gttgccctcg atccttccgc gcttgtcgct cgatgagtcg atcacgacaa
     8221 cgaagattca ttcagtggcc ggaacgctgc ctgccggtgc cggcctgatt accgtccgtc
     8281 cgtttcgcgc gccgcatcac accatttccg tggcgggtct cgtcggcggt gcatcgctgc
     8341 cgcggcccgg tgaagtaagc ctcgccaatc acggagtgct ctttctcgac gagctcgccg
     8401 agtttcagcg cacgactctc gaagtattgc ggcaaccgat ggaggatggg cacgtcacga
     8461 tcgcgcgcgt gcagaggacg ctgacgttcc cgtcgcgctt cacgctcgct gccgcgctga
     8521 atccgtgtgc gtgcgggtac ttcaatgact cgcggcgcca gtgcatttgc tcgccgggac
     8581 agatcgcgcg ctacctcggc aagatttccg gtccgctgct cgaccggatc gatcttcagg
     8641 tcgaagtccc ggcgctcgcc gtcgaggaga tctcgacgtt tacgccgggc gagaattcat
     8701 cgtcgatccg cgatcgcgtc gaagcggcgc gagagattca acggcagcgc tttgcgcgcg
     8761 tttcgtggaa gtcgaacgcc gagatgacgt cacgggcgat gcgaaaatac tgcgagctcg
     8821 accccgcgtc gcgccgtatt ctcgaccagg ccctcacgca cctcgggctc tccgttcgcg
     8881 cacacgaccg cgtgctcaaa gtcgcccgaa cgatcgccga cctcctcggc agcgaaaaaa
     8941 tccagagcgc gcacgtagcc g
//
LOCUS       DATLVW010000817         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_78377, whole genome shotgun sequence.
ACCESSION   DATLVW010000817 DATLVW010000000
VERSION     DATLVW010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78377"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VNN25_23945"
     CDS             complement(<1..1165)
                     /locus_tag="VNN25_23945"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HXH94649.1"
                     /translation="MQDHESDMPISLRNDEIGKQTAVRHGWRLNTHMSRKAVEIGNRN
                     RNDATVAKVADGVGMRINALADNGQSLDSAFEVSKHLYQYGQRATGETHGVVLTKSHI
                     VRLILDLANYSSDRNLTKLRLLEHSCGEGAFLVEAVRRLLASAKRHRAHPTELRDAIR
                     AYDIDSVSVTNTRDSIRQTLIESGVDHDTAETLVGTWIRHGDFLLASIDELFDVIVGN
                     PPYVRIEQLDQELQTLYRSRFETLFDRADLYVAFIERSLELLSLTGVLSFICADRWTL
                     NRYGAPLRGLISREYGVKVYVDLHHASPFESDVIAYPSILALERRKAEHVVFATLSEG
                     SARECRALTTLLEEGSQTSTTRGLHVSRYERWFESDEPWVLGTPKHLVALRDLE"
     gene            complement(1466..2851)
                     /gene="zwf"
                     /locus_tag="VNN25_23950"
     CDS             complement(1466..2851)
                     /gene="zwf"
                     /locus_tag="VNN25_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXH94650.1"
                     /translation="MTAVQSDALVFFGATGDLAYKMIFPALQAMIKRGNLNVPVIGVA
                     KAGWSLDQLRERARASLTEHGGGVDEEAFAKLVAQLQYIDGDYKDDATFDHLRQALGS
                     AEHPLHYLAIPSSMFAAVAKQLGRSGCAKGARVVIEKPFGRNLATALELNAALLTVFD
                     ERAIFRVDHFLGKEPVQNLLYFRFANSFLEPVWNRNFVRSVQITMAEDFGIQGRGAMY
                     EETGAIRDVVQNHLLQVVASLAMEAPQSGTAEEVRDARARILNAIQPIDPANVVLGQF
                     RGYHDEKGVAPDSKTETFAALRLEIASWRWAGVPFYIRAGKSLPVTCTEVLVELEPPP
                     LTIFRGDTVTPDSANYVRFRLGPDVAIALGVRSKVPGEGMAGQNVELLAVSKVQGQLG
                     PYERLLGDAMKGDATLFAREDMVEAEWRVVDPIIEHNIPVHEYEPGTWGPDESQRIIP
                     GRSWHEPVLTP"
     gene            complement(2964..>3915)
                     /locus_tag="VNN25_23955"
     CDS             complement(2964..>3915)
                     /locus_tag="VNN25_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94651.1"
                     /translation="GLSGYLAGRGIIFNGIGLLSMALNFQTLSSSATNDSAYPWNVPT
                     YMAIAYYHHKLSPELQKAIDGCQGNPNAPCAPLVQAEQWAITDYAAALSRADSMSPQE
                     HQSVVDKLSQYTGLSKQVIEEFNLRIDVGTFDHYLLLDQRMRVGRLDGRFKLPEPGFP
                     GGRGGGGGGGADPASTATTGPFTMAFNNYMRTELNYKTETPYYTSAQQSGMFRWSSSG
                     ATPAPGGNQGFQEEVSPLRAAIVGNPFLKVLVMEGYYDLATPYYQAEYTMHELMLPPA
                     YRSNITYERYWSGHMVYLEQKAHAKLQKDWDNFIEQTQGH"
BASE COUNT          768 a         1162 c         1190 g          795 t
ORIGIN      
        1 cctcgagatc tcgaagtgct accaagtgct tcggcgtgcc cagaacccac ggctcatctg
       61 attcaaacca ccgttcatac cgagaaacat gcaaccctct cgtcgtagaa gtctggctac
      121 cctcttcgag cagcgtcgta agagctcggc attctcgcgc tgagccttcg ctcagggtcg
      181 caaacacgac atgttcggcc ttcctgcgct ccaaggcgag aattgacgga taggcgataa
      241 catctgattc gaaaggcgac gcatgatgga ggtcgacata tacctttacg ccgtactcgc
      301 gcgagatgag gccacgcaaa ggcgcaccat agcggttcaa cgtccatcga tcggcacaga
      361 taaaggacag cactcccgtc agcgacagca gctcgagtga cctctcgatg aacgcgacgt
      421 acaagtcggc gcgatcgaag agcgtctcaa acctgctccg atagagcgtt tgtaactctt
      481 ggtccagttg ttcgatgcga acatagggcg gatttcccac gatcacgtcg aacagttcgt
      541 cgatggatgc gagcagaaag tcgccgtgcc gaatccacgt tccaacgagt gtctcggcag
      601 tatcgtgatc gacaccgctc tcgatgagcg tctgtcggat ggaatcgcga gtgttcgtga
      661 ccgaaacgct gtcgatatcg taagcgcgta tcgcgtcacg tagttcagtt ggatgggctc
      721 tgtgccgttt tgcagaagcg aggagacggc gcactgcttc gacaaggaac gccccctcgc
      781 cgcaggaatg ctcgagcagg cgcagtttcg tgaggttgcg gtcggagctg tagttcgcca
      841 aatcgaggat cagtcgaacg atgtggcttt ttgtcaaaac aacgccgtgg gtctctccag
      901 ttgctcgctg cccatattga tagaggtgtt tagacacctc gaaggctgaa tcaagactct
      961 gaccgttatc ggccagcgca ttgatgcgca tgccaacccc gtccgccacc ttcgcgacag
     1021 tcgcatcgtt tcgattgcga ttgccgatct cgacagcctt ccggctcatg tgggtgttca
     1081 gcctccagcc gtgacggacc gctgtctgct tgccgatttc gtcgttcctt agagagattg
     1141 gcatatcgga ttcatgatct tgcactagct aagatctgaa acgtcgcaag tgcacgttcg
     1201 gtcgcggatt cctcttcgac cttccttgct cgttcgcatg agtcagcaac ggatcacctc
     1261 cgagggtacg gcgttccttc atcgcaagca agaacggtcg cgacgaatct gcacgcctcg
     1321 ggcccgaggt tacctcggtg ctcccggaac accgaggagt tctacccgct gtggagtgaa
     1381 agcaaatggg acgagaacat catcgatctt cgacttcaga ggatcctcac cttcctccgg
     1441 tccctgaaga ccgcgtttgt tgttttcacg gagtcagaac cggctcgtgc cacgagcgac
     1501 ccggaatgat gcgctgcgat tcgtcggggc cccaggtgcc gggttcgtac tcgtgaaccg
     1561 ggatgttgtg ctcgatgatc ggatcgacga cgcgccattc ggcttcgacc atgtcctcgc
     1621 gtgcgaagag ggtagcgtcc cctttcatgg catcgccgag cagacgctcg taaggaccga
     1681 gctgaccctg caccttgctc acggcgagga gctcgacgtt ctggccggcc atcccttcac
     1741 cgggcacttt cgagcgtacg ccgagggcga tggcaacgtc ggggccgagc cggaaacgga
     1801 cgtagttcgc gctgtcgggc gtgaccgtgt caccgcggaa gatcgtcagt ggcggcggct
     1861 ccagctcgac gagcacctcg gtgcacgtga ccggtaacga cttccctgcg cggatgtaga
     1921 aggggacgcc ggcccatcgc caactggcga tctcgaggcg caatgccgcg aaggtttcgg
     1981 tcttcgaatc gggagcaacg cctttctcat catggtagcc gcggaactgg ccgagaacga
     2041 cgttggccgg atcgatcggc tggatcgcat tcaggattct tgcgcgcgca tcgcggacct
     2101 cttccgccgt cccgctctgc ggcgcctcca tggccagact ggccacgacc tgaagcaggt
     2161 ggttctgcac aacgtcgcgg atggcgccgg tctcctcata catcgcaccg cgaccctgaa
     2221 tcccgaagtc ctcggccatc gtgatctgaa cgctgcgcac gaaattgcgg ttccagaccg
     2281 gctccagaaa ggagttggca aagcggaagt agaggaggtt ctgcaccggc tccttgccga
     2341 ggaagtgatc gacgcggaag atggcccgct cgtcgaagac ggtgagcagc gcggcgttga
     2401 gctcgagcgc cgtggccaag ttgcgcccga acggtttctc gatgacgacg cgggccccct
     2461 tcgcgcagcc ggagcgcccg agctgtttcg ccaccgcggc gaacatgctg gaaggaatag
     2521 ccagatagtg cagcggatgc tccgcgctgc cgagcgcctg acgcagatgg tcgaaggtgg
     2581 cgtcgtcctt gtaatcgcca tcgatatatt gcagttgcgc gacgagtttc gcgaaggcct
     2641 cctcgtcgac accgccgccg tgctcggtca gactggcccg agcccgctcg cgcaattgat
     2701 cgagagacca ccccgctttg gccactccga tcaccggcac gttcaggttg ccgcgtttga
     2761 tcatcgcctg caaggcaggg aagatcatct tgtaggcaag atcgcccgtc gccccgaaaa
     2821 agacgagcgc atccgattga actgcggtca tgaacgttgc tcccgtaagt ggggtcaggt
     2881 ctaaagtcta aagtgccgaa gacgcttgat cgccctggcg acgatcgtcc tcggcacttt
     2941 agactttaga cctgaccctt cgatcaatgc ccctgcgtct gttcgatgaa gttgtcccag
     3001 tccttctgca acttcgcgtg cgctttctgt tcgaggtaga ccatgtggcc tgaccagtag
     3061 cgctcgtaag tgatgttgga gcgataggcc ggcggcagca tcagttcgtg catggtgtat
     3121 tcggcctggt agtacggcgt ggccaggtcg taatagccct ccatgacgag gactttcagg
     3181 aatggattgc cgacgatggc cgcgcgcaga ggactgacct cctcttgaaa gccctggttg
     3241 ccgccgggag cgggcgttgc gccggacgat gaccaccgga acattcccga ttgctgcgcc
     3301 gaagtgtagt aaggagtctc ggtcttgtaa ttgagctccg tgcgcatgta gttgttgaac
     3361 gccatcgtga acgggcccgt cgtcgcggtc gacgccgggt cggcgccgcc gccaccgccg
     3421 ccgcctcgcc cgccggggaa gcccggctca ggaagcttga aacgtccgtc cagtcgaccg
     3481 acgcgcatcc gctgatcgag cagcagatag tgatcgaatg tgccgacatc gatgcgcaga
     3541 ttgaactcct cgatcacctg cttcgaaagg ccggtgtatt gcgagagttt gtcgacgacc
     3601 gattgatgct cctgcggcga catcgagtcg gcacgggaga gcgcggcggc gtagtcggtg
     3661 atggcccact gctccgcctg caccagcggg gcacagggcg cgttcggatt gccctgacaa
     3721 ccgtcgattg ccttctgcag ctcgggcgag agtttgtgat ggtagtaggc gatggccatg
     3781 tacgtcggaa cgttccatgg atacgccgaa tcgttggtgg ccgatgacga gagagtctgg
     3841 aagttgaggg ccatcgacag aagcccgatg ccgttgaaga tgatgccgcg gccggccaaa
     3901 tacccggaaa gaccg
//
LOCUS       DATLVW010000818         5010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_445093, whole genome shotgun sequence.
ACCESSION   DATLVW010000818 DATLVW010000000
VERSION     DATLVW010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445093"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VNN25_23960"
     CDS             complement(<1..215)
                     /locus_tag="VNN25_23960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94652.1"
                     /translation="MKRNITDVLRRGLDTTIANWPVIVLRIAESLVFAAIVIAGILAA
                     IVPAIVAAGLSADDIRNSSDPGGAVVE"
     gene            complement(212..952)
                     /locus_tag="VNN25_23965"
     CDS             complement(212..952)
                     /locus_tag="VNN25_23965"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94653.1"
                     /translation="MSSALLPATCARCNGSGWVPLSGDSLRVEPCGCQGDLRRRQRIS
                     ASNIPRRYYPHCTIETFYDKNNAILKSARNRVREFVDSWPMTNEGRGLLLMGGCGAGK
                     THLAVAALVEIIHSGKPGRVLFCNFQELIQDIQASFDADAAVGKSEILRPLLEADLLV
                     LDELGSVKPTQFVQDLLYYVINSRYNTVRPTIFTTNATDEPGADGQRLVDKIGERLRS
                     RLYEMTEQIVIKGVPDHRSANKQSGNIL"
     gene            1091..3424
                     /locus_tag="VNN25_23970"
     CDS             1091..3424
                     /locus_tag="VNN25_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94654.1"
                     /translation="MASPLETYLASRSDTATFLLGSELGRTLEEIFPRADTLLPRPFK
                     IFPADETQEPAMGFPVADVPVLRELDAKLDRWLSDEIAWLAGRQPAAKEKAQIANGVY
                     MTQLLRMAENALMSNLLSDYHAVFWLAHSFDLARQLSSIPRRVSLYDAATGKTQGDAF
                     KYRIYAKWANDTREGMAQLATKASSILDGEEQRSLQFFKLLTDDVLILTEEFIGPDLR
                     ELRSFVNGYLRRDFQAFRDSVERMRETALDLLQRDRTFRAALPLFGAAPDQGITIALL
                     LDTRFQNFLFEQNAVQSAVTREDREQFQLIARRIREFAILNQLRRGITMMTVRSDNQV
                     VSADKRATLYSRSTRPMDFGRPGVVDPMVHRFGLIYDISNFTETLGNIRRGGRKEEIS
                     SYRQMLLFQRKLDSIAGRHLLQFEKFLGDGAFYTTRRALRLVRAAVEIQRCYADVRRK
                     GFAFNKGLRIALNYGYYRLLPMKGSGNERITEFYGPGIVELSRLTTGKANKELEEFAS
                     FLLTHGYESGKVQAFFAPLAKGVDVIDHTQHAREFYAYVNASGHLVNEGIVASFSLLQ
                     ELSNDLASEGQQLYRLRSAWATYIGFAPSLTGLEYIGVRLVGMVSLKGLEEIDVGEIV
                     PFAPGDVEATPIDGADPLIVVIRQEFHQDRDLEVPLNASAAFDDTTTSEQAIESELVI
                     CLRNEPDGTEDDVVIGQWNPRTDELRCPLRFPHGDFVRLFTLKGDVTADLLSERRETV
                     RDLYNRLSDRAPTPAVPLAEFRDLYATFVLGAVVEKL"
     gene            3584..4297
                     /locus_tag="VNN25_23975"
     CDS             3584..4297
                     /locus_tag="VNN25_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94655.1"
                     /translation="MLAILLTFLLARAKVAELTAPEIQAYGEALSALESRKPGATVGK
                     LFDAALAVRSSLLREAGNESLIEGLSDVAFKSLTHRLPGIILNREEVLLAEPDPKFFL
                     SLARRRGSAADVEFFQNYAATRPDGVWKSYTDQQTDVTGCTNFANGELVKRYGGWLAF
                     QSTYPHAYSSVVREELAAIESEVSESTCACSTRTEVLRELERFARMFPEAAVTARVRN
                     RIRAIRALSSSIRFNCQSG"
     gene            4463..>5010
                     /locus_tag="VNN25_23980"
     CDS             4463..>5010
                     /locus_tag="VNN25_23980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94656.1"
                     /translation="MRVDHEKREILCSVGDLVYESTNRRIGVERGDGFRRMWIGQDIH
                     TKRAEQRTGEDANYRAEVHVVHRTQLGAWSVTITGRIDGLSVDKAEKRVTIEEVKSIH
                     FDLELEALYRSDKLQRHLLQLLLYSYFLSGQPDFEGFVFAPQLVLIDLISGDAKTIDA
                     EFDKEVVLGTLNERLEGLITEI"
BASE COUNT          966 a         1585 c         1513 g          946 t
ORIGIN      
        1 cactcgacga ccgcgccgcc gggatcggac gaattcctga tgtcgtcggc cgacaagccg
       61 gccgcaacga tggcgggcac gattgcagcc agaatcccgg cgatgacaat cgcggcaaac
      121 acgaggctct cggcgatgcg caatacgatg accggccagt tggcgatggt cgtatcgagc
      181 ccgcggcgga gaacgtcggt tatgttgcgt ttcacaggat gttgccgctc tgtttgttcg
      241 cgctgcggtg atcggggacg cccttgatca cgatttgctc ggtcatctcg tagagccggc
      301 tgcgtaaccg ctcgccgatc ttgtccacca gccgctgacc gtcggcgccg ggctcgtcgg
      361 tcgcgttggt cgtgaaaatc gtgggacgca cggtgttgta gcggctattg atcacatagt
      421 aaagaagatc ctgcacgaac tgcgtcggct tcaccgatcc gagctcgtcg agaacgagga
      481 ggtccgcttc cagcagcggg cggaggattt ccgacttgcc cacggcggcg tcggcatcga
      541 agctggcctg aatgtcctgg atcagctcct ggaagttgca gaagagcaca cgccccggtt
      601 tgccactgtg aatgatctcg accagcgctg cgacagcgag atgcgtcttc cccgcgccgc
      661 agccgcccat gagcagcagg ccgcgtcctt cgttggtcat cggccaggag tcgacgaact
      721 cgcgcacgcg attccgcgcg gacttcagga tggcgttgtt cttgtcgtag aacgtctcga
      781 tcgtgcagtg cggatagtag cggcgcggaa tgttcgacgc ggaaatgcgc tgccgccgcc
      841 gcaaatctcc ctgacatccg cacggctcca cgcgcaacga gtcgccggag agcggaaccc
      901 agcccgatcc attgcagcgt gcgcaggtgg cggggaggag tgcggaggac atggttgaag
      961 tttagtcgag agagccggga tgttgaagat tacgtgctcg ccgggactgg gttgcatgaa
     1021 gtcgaaaatc caaccttccg cctttgcttt gtccaagcgt cctcttcgac cataattctc
     1081 cccgcgtcca atggcttcac ccctcgagac gtacctcgcc tcgcggtccg ataccgccac
     1141 gttcctcctc ggcagcgagt tagggcggac gttggaggag attttcccgc gggcggatac
     1201 gctgctgccg aggccgttca agatcttccc ggcggacgag acgcaggagc cggcgatggg
     1261 tttccctgtc gccgacgtgc cggtgttgcg cgagctggac gcgaagctcg accgctggct
     1321 ctccgatgag attgcctggc tggccggccg gcagcccgcg gcaaaggaaa aggcccagat
     1381 cgcgaacggc gtgtacatga cgcagctcct gcgcatggcc gagaacgcgt tgatgtcgaa
     1441 ccttctcagc gactaccacg ccgtcttctg gctcgcccat tcgttcgatc tggcgcggca
     1501 actgtcgtcg atccccaggc gcgtcagtct ctatgatgcg gcgaccggca agacccaggg
     1561 cgatgccttc aagtaccgga tctacgccaa gtgggcgaac gatacgcgcg aaggaatggc
     1621 gcaactggcc acgaaagcgt cgtcgattct cgacggcgag gaacagcgca gcctgcagtt
     1681 cttcaagctc ctcaccgacg acgtgctcat cctgaccgag gagttcatcg gtcccgattt
     1741 gcgcgagttg cgctcgttcg tcaacggcta tctgcgtcgc gatttccagg cgttccgcga
     1801 ctcggtcgag cggatgcgcg agaccgcgct cgacctgctc cagcgcgatc gtacctttcg
     1861 cgcggcactg ccgctcttcg gtgccgcacc cgaccaggga atcacgatcg cgcttctgct
     1921 cgacacgcgc tttcagaatt tcctgttcga gcagaatgcg gttcagtcgg cggtgacgcg
     1981 cgaggaccgc gagcaattcc aactcatcgc acggcggatc cgcgagttcg ccatccttaa
     2041 ccagttgcgc cgcggcatca cgatgatgac tgtgcgctca gacaatcagg tcgtttcggc
     2101 cgacaagcgg gccacgctct actcccgctc gacgcggccg atggatttcg gccgtccggg
     2161 cgtggtcgat ccgatggtgc atcgcttcgg actgatctac gacatctcca acttcaccga
     2221 gacgctcggc aacatccgcc gcggcgggcg gaaggaggag atcagctcct accggcagat
     2281 gcttctgttc caacgcaaac tcgattcgat cgccggccgg cacctcctcc aattcgagaa
     2341 gttcctcggc gacggcgcct tctacacgac gcggcgcgcg ctacggctgg tgcgcgcggc
     2401 cgtggagatt cagcgctgct acgccgacgt ccgccgcaaa ggtttcgcgt tcaacaaggg
     2461 gctgcgcatt gcgctcaact atggctatta ccggctgctg ccgatgaagg gaagcggcaa
     2521 cgagcggatc acggagtttt acgggccggg catcgtcgag ctttcgcgcc tcacgaccgg
     2581 caaggccaac aaggagctcg aggagtttgc ttcgtttctt ctcacgcacg gctacgaatc
     2641 ggggaaggtg caggcgttct tcgcaccgct cgcgaaaggc gtcgacgtca tcgaccacac
     2701 gcagcatgcc cgtgagttct acgcctacgt gaatgccagc ggccacctcg tcaacgaagg
     2761 gatcgtggct tccttctcgc tgctgcagga gctgtcgaac gatctggcgt ccgaggggca
     2821 gcagttgtac cgattgcgct cggcgtgggc gacatacatc ggattcgcgc catctctgac
     2881 aggactggag tacatcggag tccgcctcgt cggcatggtg tcgctgaagg gactggaaga
     2941 gatcgacgtt ggcgagatcg tgccgttcgc gccgggcgat gtcgaagcaa ctcccatcga
     3001 cggcgcagac ccgctcatcg tagtgatccg ccaggagttt catcaggacc gcgacctcga
     3061 agtcccgctg aatgcgtccg ccgccttcga cgacacgacg acgagcgagc aagccatcga
     3121 atcagagctc gtgatctgcc tgcgcaacga acccgacggc accgaagacg acgtcgtcat
     3181 cggccagtgg aacccgcgca cagacgagct gcgctgccca ctccgctttc cgcacggcga
     3241 cttcgtccgc ctcttcacgc tcaaaggcga cgtcaccgcc gacctcctca gcgagcgccg
     3301 cgaaaccgtc cgcgatctct acaaccgcct ctccgaccgc gcgccaacgc cggccgtgcc
     3361 cctggccgag tttcgcgact tgtacgcgac gttcgtgttg ggcgccgtcg tcgagaagct
     3421 gtagtgaaat gcccaatggt tcacggattg atttgagggg cacgtacggg cggcgaggag
     3481 acttttcgcg caatgtcgat cgaaacgcgg cggcccagaa gattcttcgg cgcttcagcc
     3541 cgacagcggg ctatcctcat cgccgttgca gccgaaccat tcgatgctcg caattctgct
     3601 cacgttcctg ctagcccggg cgaaagtcgc cgagttgacc gcgcccgaaa ttcaggcata
     3661 cggcgaagcg ctgtccgctc tggaatcgag gaaacccggc gccacggtcg gcaagctatt
     3721 cgatgcggca ctcgctgtcc gatcgagcct gttgcgagaa gcaggcaacg aatctctcat
     3781 cgaagggctt tctgacgtgg cgttcaagtc tctcacgcac agacttcccg gcatcatcct
     3841 caatcgggaa gaggttcttc tcgccgaacc agacccgaag ttctttctgt cccttgcgcg
     3901 gcgccgtgga agcgcagctg atgtcgagtt ctttcaaaac tacgcggcta cccgccccga
     3961 cggtgtgtgg aagtcgtaca ccgaccaaca aactgacgtt acaggatgta cgaatttcgc
     4021 gaacggtgag ctggtgaagc gttacggcgg ctggcttgcg ttccagtcaa cataccctca
     4081 cgcatactca tcggtggtta gagaagagct cgcagctatc gagagcgaag tctcggaaag
     4141 cacctgcgca tgcagcacgc gcaccgaagt ccttcgcgag ctggaacgat tcgcgcggat
     4201 gtttccagag gcggccgtca cagcaagagt gcgaaaccgc atccgtgcca ttcgcgcgtt
     4261 gagctcttct attcgcttca actgccagtc aggatgaact gaacgacaag ctcgcgagat
     4321 cgtcgaaccg tttctggaat cctagggggc aatttcccat cttcgcctca atctcgacaa
     4381 cgcggcgcca gccgcactca accgacaacg gacaacgcgt gccgcgttag cggtacgcaa
     4441 ttcggttacc atcaataacc caatgcgcgt cgaccatgag aagcgggaga tcctctgttc
     4501 ggtcggcgat ctcgtttacg aatcgaccaa ccggcgcatc ggcgtcgagc gcggggacgg
     4561 gttccggcgg atgtggatcg ggcaggacat ccacacgaag cgcgcggaac agcggaccgg
     4621 tgaggacgcc aattaccgcg ccgaggtgca tgtcgtccac cgaacacaat tgggggcgtg
     4681 gtcggtcacc atcaccggcc gcatcgacgg actctccgtc gacaaagcgg aaaagcgcgt
     4741 caccatcgaa gaggtgaagt cgatccactt cgatctcgag ctcgaagcgc tctaccgatc
     4801 cgacaagctc cagcgccacc tcctccaact cctgctctat tcgtatttcc tttccggcca
     4861 gccggacttc gaaggcttcg tcttcgcgcc gcagctcgtg ctcatcgacc tcatctccgg
     4921 cgatgcgaaa acgatcgatg cggagttcga caaggaagtc gtcctcggca cgctgaacga
     4981 gcggttggaa ggtctcatca cagagatcga
//
LOCUS       DATLVW010000819         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404638, whole genome shotgun sequence.
ACCESSION   DATLVW010000819 DATLVW010000000
VERSION     DATLVW010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404638"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..719)
                     /gene="ispD"
                     /locus_tag="VNN25_23985"
     CDS             complement(27..719)
                     /gene="ispD"
                     /locus_tag="VNN25_23985"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXH94657.1"
                     /translation="MPDAERVLVIIPAAGSGVRFGGDVPKQFLPLGGKPLVQRVVERF
                     LFDAVVTRVVVPVLDMLLNAVKESPGDRVKFVAGGATRLQSVAAGLAAAGDEFDLVAI
                     HDAVRPFFSPDTFHATLDAARTHGAAFPGIPLPDTIHEIKDDAIASTPDRSVFVAAQT
                     PQCFRIDVLRDILTRAAREDANATDEAGLAAKYGHRVQLVPGDSMNFKITRPEDLVMA
                     ERIYAEWGGGEE"
     gene            complement(761..1318)
                     /gene="frr"
                     /locus_tag="VNN25_23990"
     CDS             complement(761..1318)
                     /gene="frr"
                     /locus_tag="VNN25_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXH94658.1"
                     /translation="MINDVIKDIQHRMHGAIEALHREFKTLRTGRANASMLDGIMVDY
                     YGTPTPIAQVASLKVPEASLIVAEPWDKSMVAAVEKAIRTSDLGLNPSTDGKVVRIPI
                     PTLTEERRKELVKRGHHMAEESRTAIRQVRRDGNDKLKKMLKGHEISEDDERHALDEI
                     QKMTDKHIDEIGSILKSKETDIMAV"
     gene            complement(1358..2086)
                     /gene="pyrH"
                     /locus_tag="VNN25_23995"
     CDS             complement(1358..2086)
                     /gene="pyrH"
                     /locus_tag="VNN25_23995"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXH94659.1"
                     /translation="MPRYKRILLKLSGEALMGGRRTGIDPETIARIADEIVDVHRAGV
                     EIAIVIGGGNIFRGVTAATEGIDRVAGDHMGMLATVINAIALQDALERRGAQTRVTSA
                     ITMAEVAEPFIRRRAIRHLEKSRIVICAAGTGNPYFTTDSAAALRASELKCAIIFKAT
                     KVDGIYTADPEKDPSATRIPHVTYQRVLAERLEVMDATAIDLARGSSIPIYIFSLREP
                     GNIRKALLGEDIGSIVSDEIPGKG"
     gene            complement(2155..>2681)
                     /gene="tsf"
                     /locus_tag="VNN25_24000"
     CDS             complement(2155..>2681)
                     /gene="tsf"
                     /locus_tag="VNN25_24000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXH94660.1"
                     /translation="SIKENIAIRRFVRYEAAPNAVFSAYIHAGGRIGVLVELVATSGA
                     KSAKMNEVAKDVAMHVAAAEPRFIRRDEVTQKDLDTEREIARDAAAKSGKPENIVEKM
                     VTGKMEKFYGEACLLEQPYIRNDKQTVNEYLASSAKEYGCEYTITRFTRYKLGEGIEK
                     KSDDFAAEVASFMK"
BASE COUNT          466 a          819 c          835 g          561 t
ORIGIN      
        1 ttcgcgcgaa aaatggctcg ggctcttcat tcttcgcctc cgccccattc cgcgtaaatg
       61 cgctcggcca tgacgagatc ctcggggcgc gtgattttga agttcatcga atcgccaggg
      121 acgagttgga cgcggtgacc gtactttgcg gccagccccg cctcatcggt cgcgttcgcg
      181 tcttcacgcg ccgcgcgtgt gaggatgtca cggagaacgt cgatgcgaaa gcattgcggc
      241 gtctgtgcgg ccacgaaaac actccgatcg ggagtcgaag cgatggcgtc gtctttaatc
      301 tcatgaatcg tgtcgggcag cgggatgccg gggaacgcgg ctccgtgcgt tcgcgcggcg
      361 tcgagcgtgg cgtgaaacgt atcgggcgag aagaacggac gcaccgcatc gtgaatggcc
      421 acgagatcga actcgtcacc tgccgcagca agtcccgcgg ctaccgattg cagccgcgtc
      481 gctccgccag cgacaaattt cacgcgatcg cccggcgatt ccttcacggc gttgagcagc
      541 atgtccagca ccggaacgac cacgcgcgtg acgacggcat cgaagaggaa ccgttccacc
      601 acgcgctgca cgagcggttt gccgcccaac gggagaaact gcttcggaac gtcgccaccg
      661 aagcgcacgc ccgatccagc ggcagggatg atgactagaa cccgttcggc gtccggcatg
      721 gtctaacggt ctcgcagttc gctagcccgc aaccgcacca tcacaccgcc atgatgtcgg
      781 tttctttgct cttgaggatg ctgccgatct cgtcgatgtg cttgtcggtc atcttctgga
      841 tctcgtcgag ggcatgcctc tcgtcgtcct ccgagatctc gtgacctttg agcatcttct
      901 tcagcttgtc attgccgtcg cggcgcacct ggcggatggc ggttcgcgat tcctcggcca
      961 tgtggtgccc gcgcttgacc agctctttgc gccgttcctc ggtgagcgtc ggaatcggga
     1021 tgcgaaccac tttgccgtcg gtggatggat tcaatccgag gtcggaggtg cggatggctt
     1081 tctcgacggc ggcgaccatc gacttatccc acggctcggc cacgatcagc gacgcctcgg
     1141 ggaccttgag cgacgccacc tgcgcgatcg gcgtcggcgt gccgtagtaa tcgaccatga
     1201 tcccgtcgag catggaagcg ttggcccgtc cggtgcgaag cgttttgaac tcgcggtgaa
     1261 gagcctcgat ggcgccgtgc atacgatgct gaatgtcttt gatgacgtcg ttgatcatag
     1321 gaacctcgtg aatcgtgaag ggtgaatcat gaaggattca tcctttcccc ggtatctcgt
     1381 cagaaacaat cgagccaata tcctcgccca gcagcgcctt gcggatgttg cccggctcgc
     1441 gcagcgagaa aatatagatc gggatcgagc tgccacgcgc aaggtcgatc gcggttgcgt
     1501 ccatgacttc caaccgctcg gcgagcacgc gctggtaggt cacatgcgga atccgcgtcg
     1561 ccgaggggtc tttctccgga tcggcggtgt aaatgccatc gaccttcgtc gccttgaaga
     1621 tgatcgcgca cttcaattcg ctggcgcgca gtgcggcagc ggaatcagtc gtgaagtagg
     1681 ggttgccggt gccggcggcg cagatgacga tgcgcgattt ctcgagatgg cgaatggcgc
     1741 gccggcgaat gaacggctcg gccacctccg ccatcgtgat cgccgacgtc acccgtgtct
     1801 gcgcgccgcg acgctcgagc gcgtcctgca gcgcgatggc gttgatcacg gtggccagca
     1861 tccccatgtg atcgccggca acgcggtcga tgccttccgt ggccgcagta acgcctcgaa
     1921 agatgttccc gccgccgatg acgatggcga tctcgacgcc ggcgcggtgt acgtccacga
     1981 tctcgtcggc aatgcgcgcg atcgtttcgg gatcgatgcc ggtgcggcgc ccgcccatga
     2041 gcgcttcacc ggagagtttg agaaggatgc gtttgtagcg aggcattggc gacgctttat
     2101 ataacaaaaa gggcggccta tccggccgcc cttagttgat gtcggaatcg ctgtctattt
     2161 catgaacgac gccacttccg cggcaaagtc atcgctcttt ttctcgatgc cttcgccgag
     2221 tttgtagcgg gtgaaacggg tgatcgtgta ctcgcagccg tactctttgg ccgacgacgc
     2281 cagatactcg ttcacggtct gcttgtcatt gcggatgtag ggctgctcga gcagacaggc
     2341 ctcgccgtag aacttctcca tcttgccggt aaccatcttc tcgacgatgt tctccggctt
     2401 gccggacttg gcggcggcgt cgcgtgcgat ctcgcgctcg gtgtcgagat ccttctgcgt
     2461 cacctcgtcg cggcgaatga agcgcggctc ggcggcggcg acgtgcatcg ccacatcctt
     2521 ggcaacctca ttcatcttgg cgctcttcgc accgctggtg gccacgagct cgacgagcac
     2581 gccgatgcgt ccacctgcgt ggatgtacgc cgaaaagaca gcgttcggtg cggcttcgta
     2641 gcggacgaaa cggcggatgg cgatgttctc tttgatcgat g
//
LOCUS       DATLVW010000820         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_445164, whole genome shotgun sequence.
ACCESSION   DATLVW010000820 DATLVW010000000
VERSION     DATLVW010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445164"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VNN25_24005"
     CDS             complement(<1..310)
                     /locus_tag="VNN25_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94661.1"
                     /translation="MSEITILVVDDNPTNLKLVADVLAFDGYRILKAGDAEEAQELIK
                     TSPPDLILMDIALPGMDGLTLTRQIKANDETHDIVVVALTAFAMKGDDEKARAAGCDG
                     Y"
     gene            complement(307..1446)
                     /locus_tag="VNN25_24010"
     CDS             complement(307..1446)
                     /locus_tag="VNN25_24010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94662.1"
                     /translation="MTSVPQQPEDQFRGLLEAAPDAMVIVNQAGSIVLVNTQTERLFG
                     FSREELLGKRIEVLIPVRFHAHHGSHRDGYFADPHVRPMGAGLDLYGLRRDGTEFPVE
                     ISLSPLRTPEGLLVTSAIRDISDRKRIERALMEKNNELERANQAKDRFLASMSHELRT
                     PLNGIIGFAEFLADGRPGTVNEKQKEYLEDILKSGQHLLHLINDMLDLVKVQAGKLEL
                     SPEVFEIRSGIEEVCAVVHPIAEQKNIDVRIEIAPGLGTITLDLQRFRQILYNLLSNA
                     LKFTDDGGVVTIKADRRADGNIRLAVRDSGIGIAADDIPRLFTEFEQLETGIARRFGG
                     TGLGLALTRSIVTLQGGSIDVESEIGIGSTFTVALPQGSLEEAGA"
     gene            1804..2457
                     /locus_tag="VNN25_24015"
     CDS             1804..2457
                     /locus_tag="VNN25_24015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH94663.1"
                     /translation="MPRIFDTSALKTFVTCKRTSRVYQQGSILFNEGDAPEGLHILDS
                     GRAKMFGSSSRGKTFISRIANAGEMLGLNAIILGKPYLVSAEAMEPSRVSYVLREDFL
                     NFLKKSPDASRQVIQQLSSSYYDSQRDVRTLSLSSSIREKIARLLVSWVESGDHPNEQ
                     VWINMDLTHEQIGQMIGASRETVSRTMSALSGIGAIVVKGHVLEIPSMTRLRELAKM"
     gene            2580..>2943
                     /locus_tag="VNN25_24020"
     CDS             2580..>2943
                     /locus_tag="VNN25_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94664.1"
                     /translation="MKKLLLSIVIIVIVVIGGLYIWNRILETKPPEPGAVLDEAKAAG
                     RDAKSMPGADEDYYADMDYGVTKNPDAVRASLDPYVPGITADAAVKSAVRGRNNWIVW
                     TAGNDRLWDELSRQSAGYL"
BASE COUNT          600 a          889 c          857 g          597 t
ORIGIN      
        1 tgtagccatc gcatccggct gcgcgcgcct tctcgtcgtc ccccttcatg gcaaacgccg
       61 tcaatgccac gacgacgatg tcgtgcgtct cgtcgttcgc tttgatctga cgcgtgaggg
      121 tcagtccgtc catgcccggc aaggcaatgt ccatcaggat gagatcgggc ggcgaagtct
      181 tgatcagctc ctgcgcctct tcggcatcgc ccgctttgag aatgcgatag ccgtcaaacg
      241 cgagaacgtc ggcgacgagt ttcaggttcg tggggttgtc gtccacgacc aggatcgtga
      301 tttcgctcac gcacctgcct cttccaggga gccctgcggc agagcgacgg tgaacgtgct
      361 accgatgccg atttcgctct cgacgtcgat cgagcctccc tgcaacgtga cgatgctcct
      421 ggtcaacgcc agtccgaggc cggtacctcc aaaacggcgg gcaataccgg tttccaactg
      481 ctcgaactcg gtgaatagcc gcgggatgtc atccgcggca attccgatac cgctgtctcg
      541 aaccgcaagc cggatgtttc catccgcgcg gcggtccgcc ttgatcgtca cgacgccgcc
      601 gtcatccgtg aacttcagcg cgtttgacag aaggttgtag aggatctggc gaaaacgctg
      661 cagatcaagc gtgatggtgc caaggccggg agcgatctcg atgcggacat cgatgttctt
      721 ctgctcagca atcggatgga cgacggcgca gacctcttcg atcccggacc ggatctcgaa
      781 gacttcaggg ctcagctcga gctttccggc ctgaactttc accagatcga gcatgtcgtt
      841 gatgagatga aggaggtgct ggccgctctt gagaatgtcc tcgagatact ccttctgttt
      901 ctcattgacc gttccgggcc tgccatccgc gagaaactcc gcgaaaccga taatgccgtt
      961 gagcggcgta cgcagctcat gggacatgct ggccaggaac cggtctttgg cctgattggc
     1021 ccgttccagc tcgttgttct tttccatcag agcgcgctcg atccgcttgc ggtcggagat
     1081 gtcgcggatg gcgctggtga cgagcaaccc ctccggcgtt cgcaacgggc tcagactgat
     1141 ctcaaccgga aactccgtgc cgtcgcggcg caagccatat aaatcgaggc ctgcacccat
     1201 cggccggacg tgcggatccg caaaatagcc gtcgcgatga cttccgtggt gggcgtgaaa
     1261 ccgaaccggg atcaacacct cgattcgctt gccgagcaat tcctcacggc tgaaaccgaa
     1321 caacctttcg gtttgtgtat tcacgaggac gatcgagccg gcctgattga caatgaccat
     1381 ggcatcgggc gcagcttcga ggaggcctcg gaattgatct tcgggttgct gcgggacgga
     1441 tgtcattagc cctcctcagc catgagcaca accgcacaat gccctgaaag cgctccgagc
     1501 cgactcgatt acttttatac tccctgctgt atggcggtaa accttctcta tggcgcttcg
     1561 tatgattctt ctcgtcaaag attcaaaccg atcggggcca gcccggaatt gaagggaatt
     1621 gcgctgcagc ggggatgaac cactcgaggc ggcaaatgat cattccatgc gatacgccgg
     1681 aagaccgtga tgtccgatac ggtgacgtga cctcagtcac ctgatccagc gactgaatat
     1741 gtttcgaaaa aactgactgc gggtgcggat tttgctactg ttagacctgc tgagctcgtc
     1801 gatatgcctc gtatattcga tacttcggca ctgaagacgt tcgtcacgtg caaacgcacg
     1861 tcgcgcgtct atcagcaggg cagcattctt ttcaacgaag gcgacgcgcc cgagggcctg
     1921 cacattctcg actcggggcg cgccaagatg ttcggatcgt cgtctcgcgg aaaaacgttc
     1981 atcagcagga ttgcgaatgc cggcgagatg ctcgggctca atgccatcat cctcggcaag
     2041 ccctatctgg tcagcgccga ggcgatggag ccttcgcgcg tgtcgtacgt tctgcgcgaa
     2101 gactttctca atttcctgaa gaagtcgccg gacgcttcgc ggcaggtcat tcagcagctc
     2161 agctccagtt actacgactc gcagcgcgac gtgcgcacac tcagcctctc cagcagcatc
     2221 cgcgagaaga tcgcacgtct cctcgtttcc tgggtcgagt cgggcgacca tcccaatgag
     2281 caggtctgga tcaacatgga tctgacccac gagcagatcg ggcagatgat cggcgcatcg
     2341 cgcgaaacgg tctcccgcac gatgtcggcg ctgtcaggaa tcggcgcgat cgtcgtgaag
     2401 ggtcacgtcc tggagattcc aagcatgaca cgccttcgcg aactggcgaa gatgtaacgg
     2461 gccgcctcgt ccgatccgac cgatccgagc agaaacagaa ctttgggtgg ggaaaccacc
     2521 ggctcaatca ctcctacata tagaaggatg aggttgcccg cctcgtcagg gagggatccg
     2581 tgaaaaagct cctgctctcg atcgtcatca tcgtcatcgt cgtcatcggc ggtctttaca
     2641 tctggaacag gattctggag accaagccac cggagccggg agcggtcctg gacgaagcca
     2701 aggccgcggg acgcgacgcg aagagcatgc cgggagccga cgaggactac tacgcggaca
     2761 tggattacgg cgtcacgaag aatccggacg ccgttcgcgc atccctcgat ccatacgttc
     2821 ccggcatcac cgccgatgcc gccgtgaaga gcgccgtgcg cgggcggaac aactggatcg
     2881 tctggaccgc cggcaacgac cggctatggg acgagctctc gcgccagagc gccggatacc
     2941 tcg
//
LOCUS       DATLVW010000821         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_487574, whole genome shotgun sequence.
ACCESSION   DATLVW010000821 DATLVW010000000
VERSION     DATLVW010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487574"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VNN25_24025"
     CDS             <1..1580
                     /locus_tag="VNN25_24025"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HXH94665.1"
                     /translation="EYGVTTGFGEFKDKPIAPADLEQLQRNLLLSHSVGVGENCDPDD
                     LSNYFPADVVRAVLATRLNSFLQGHSGVRRKLVDILQAMLDHGVIPLVPLRGSVGASG
                     DLCPLSHTFATLLGAGRFYIAGTTRLRDASELPGTAGVSPASHEAKTAGETPALPGFT
                     AEDLRPTFKEGLALVNGVNFSASMLAFGVADAERLADLADASAALTLEAMGGCTRALD
                     PKVHAARGHAGQIASADRMRALIAGSKLVERAGAVQDPYSLRCAPQVHGASRAAIRYA
                     RDVAEREINAATDNPLFFPGESEPFDVQFRDNWPPNYRGDERLAYSAGNFHGQPIALA
                     ADFLSIALAELANISERRTQMLLDSNMNRGLPSNLTTRPGVNSGLMIMQYTAASLVSE
                     NKVLAHPASVDSIPTGANAEDHVSMSTHAARKMRTILGNAQSVLAIELIVAAQAIDWR
                     VAMMQGVSEAHNLADVETQAAAFEAAVAGHAADIARFLGHGTAEHYLRIRALVDPLFA
                     DRPLHEAIRRVRLGICQ"
     gene            1586..2002
                     /gene="arfB"
                     /locus_tag="VNN25_24030"
     CDS             1586..2002
                     /gene="arfB"
                     /locus_tag="VNN25_24030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXH94666.1"
                     /translation="MRDLDLGDGIVIPGGQLRAATSRAGGPGGQNVNKVETRVTIEVD
                     VDTLPLPDDRKQRIRERLAGRINKLGVLRVTSQAERSQLANRDRALARLEELLADAIA
                     EQETRKPTRISRAQKTRRLEEKRRRAQTKKLRKIED"
     gene            complement(2095..>2288)
                     /locus_tag="VNN25_24035"
     CDS             complement(2095..>2288)
                     /locus_tag="VNN25_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94667.1"
                     /translation="PPPAESSLRFLNQQLQKLAGAVDSADAAPTTDARASWAKLKPAT
                     DAALTAWAGVKGEAPVPPK"
BASE COUNT          400 a          840 c          679 g          369 t
ORIGIN      
        1 ccgagtacgg cgtcaccacc ggcttcggcg aattcaaaga taaaccaata gctccggccg
       61 atctcgagca gcttcagcgc aacctccttc tgtcgcattc cgtcggcgtc ggcgagaact
      121 gcgatcccga cgatctctcg aactacttcc ctgccgacgt cgtgcgcgcc gtgctggcca
      181 cgcggctcaa ctcgttcctg caaggccact ccggcgtccg ccgcaaactc gtcgacatcc
      241 tccaggcgat gctcgaccac ggcgtcatcc cgctcgtgcc gttgcgcggc tccgtcggtg
      301 ccagcggcga tctctgcccc ctctcgcaca ccttcgccac gctcctcggc gctggacgct
      361 tctacatcgc cgggaccacg agactacgcg acgcatccga gctccctggg accgcgggcg
      421 tctcgcccgc atcgcacgag gcaaagacag cgggcgagac gcccgcgctc ccagggttta
      481 ctgccgaaga tctgcggcca acattcaaag agggcctcgc cctcgtcaac ggcgtcaact
      541 tctccgcatc gatgctagct ttcggcgtcg ccgatgccga gcgtctcgcc gatctcgccg
      601 atgcctcggc cgcactgacc ctcgaagcga tgggcggctg cacccgtgcg ctcgatccga
      661 aagtccatgc cgcacgcggt cacgcaggcc agatcgccag cgcggaccgc atgcgtgcgc
      721 tcatcgccgg cagcaaactc gtcgagcgtg ccggtgccgt gcaggatccc tattcgctcc
      781 gctgcgcgcc gcaagttcat ggcgcctcgc gcgcggccat ccgctacgcg cgcgacgtcg
      841 ccgagcgcga gatcaacgcc gccaccgaca atccgctctt ctttcccggc gagtccgagc
      901 cgttcgatgt tcagttccgc gacaactggc cgccgaacta tcgcggcgat gagcgcctcg
      961 cctactccgc cggcaacttc cacggccagc cgatcgcgct cgctgccgac ttcctcagca
     1021 tcgccctcgc cgagcttgcc aacatctccg agcgccgcac tcagatgctg ctcgacagca
     1081 acatgaatcg cgggttgccg tcgaacctga ccacgcgtcc cggcgtgaac tcgggactga
     1141 tgatcatgca gtacaccgcc gcctcgctcg tctccgagaa caaagtcctc gcccacccag
     1201 cctcggtcga ttcgattccg accggcgcaa acgccgagga tcacgtgtcg atgtcgacgc
     1261 acgcggcgcg caaaatgcga acgatcctcg gtaacgcgca gagcgtcctg gccatcgagc
     1321 tgatcgtcgc cgcgcaagcg atcgactggc gcgtagcgat gatgcaaggc gtgtccgagg
     1381 cccacaacct cgcggatgtg gaaacgcagg ctgcggcatt cgaagccgcc gtcgccggcc
     1441 acgccgccga catcgcccgt ttcctcggcc acggcaccgc agagcactat ctccgcatcc
     1501 gcgccctcgt cgatccgctc ttcgccgacc gcccgctcca cgaggccatt cgccgcgtgc
     1561 gcctcggcat ctgccaatag caaccatgcg cgatctcgac ctcggtgacg gcatcgtgat
     1621 tcccggcggc cagctgcgcg ctgcgacgtc ccgcgccggc ggtcccggcg gccagaacgt
     1681 gaacaaggtc gagacgcgcg tcacgatcga agtcgacgtc gacacgctgc cgctgcccga
     1741 tgatcgcaaa caacggatcc gcgaacgcct ggctggccgc atcaacaaac tcggcgttct
     1801 gcgcgtcacc tcccaagccg aacgaagcca gctcgccaac cgcgaccgcg cccttgcccg
     1861 cctggaagaa ctgctcgccg acgccatcgc cgaacaggag acgcgcaaac caacacgcat
     1921 ctcccgtgcg cagaaaacgc gacggctcga agaaaagagg cgccgcgcac agacgaagaa
     1981 gctgcggaag attgaggatt aggtttgatc cgcagatgac gcagataaga cgcagatgag
     2041 agcttcctgg ttgctcatct gcgtcctatc tgcgtcatct gcggatcagg ttttctactt
     2101 cggcggaacc ggcgcctccc ccttcacgcc cgcccaggcg gtgagcgcgg catcggtcgc
     2161 tggtttcagt ttggcccagc tggcgcgggc gtcggtggtg ggggcggcgt cggcactgtc
     2221 gacggcgccg gcgagcttct gcagttgctg attgaggaag cggagggatg actcggcggg
     2281 cggggggg
//
LOCUS       DATLVW010000822         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404740, whole genome shotgun sequence.
ACCESSION   DATLVW010000822 DATLVW010000000
VERSION     DATLVW010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404740"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VNN25_24040"
     CDS             <1..820
                     /locus_tag="VNN25_24040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH94668.1"
                     /translation="GAGKTTLIGILTTRVRITTGEAFVSGANVTTDPAAVRSRIGVVP
                     QRPNPDRSLSVIENLVFHATYFGIGRTEAARRGEQLLERMGIADKMHAKVDQLSGGQQ
                     QRLMIARALSHDPKVVFLDEPTVGLDPQARLSLWEILRELHNQGRTIVMSTHYMEEAD
                     QLCNRLAIIDQGKLLALDTPAVLKAKAPGDTLVEVTFDGDASVAADAARGMEGVSSAE
                     ARGPILRVFSKRGGELIPSLINAGEAHGRVVRDIQLSRPSLETLFISLTGRKLT"
     gene            817..1614
                     /locus_tag="VNN25_24045"
     CDS             817..1614
                     /locus_tag="VNN25_24045"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94669.1"
                     /translation="MTVEPLAPVAPSRVFFAILLRDLTVARRELPYFLVRTALQPILF
                     VTVFGFLLPRMGLIPHGYSTTMLPGVVALSLALSAIQSVSLPMMQDFGFTKEIEDRLL
                     SPVPIHLVAVEKVVGGVLQGIVAALFVLPVARLIMGPIPGLSFRNLPLLLAVTILGGA
                     AFSAMGLYLGTGIAPQQIGLMFSVIVAPMIMFGCTYYPWAGLSHVPVLKWLVLINPLV
                     YVSEGMRAALTPGIPHMPLLMILAALVLLTVLFMVLGLRAFDKRAMS"
     gene            1805..2092
                     /locus_tag="VNN25_24050"
     CDS             1805..2092
                     /locus_tag="VNN25_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94670.1"
                     /translation="MTPAKSNRGRPIVHTEPWAKITVVLLDRHVAYLDRLAIDIRLRH
                     GKAISRAEIIRGLIEAAFQSGVDLSEADSIGTLVELLTGSPMKTPKKPSSR"
     gene            complement(2089..2277)
                     /locus_tag="VNN25_24055"
     CDS             complement(2089..2277)
                     /locus_tag="VNN25_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94671.1"
                     /translation="MSNEINPAMPKAFGWPQAIRTILDRIEQSNIDLTAASSEYEIAR
                     LAAGELRSKSRRRTAPRA"
     gene            complement(2482..>2790)
                     /locus_tag="VNN25_24060"
     CDS             complement(2482..>2790)
                     /locus_tag="VNN25_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94672.1"
                     /translation="SIARRENSEIHLIIVCEEPERIASQDLLDAMTGGTFDGARVLPV
                     VLQGKDVQAELARYAKTVDADALFLNVGAHEISTLKQDIIRKVDTPVMLVPETEPLSA
                     "
BASE COUNT          488 a          854 c          879 g          569 t
ORIGIN      
        1 cggcgccggc aagacgacgc tcatcggcat cctgacgacg cgcgtccgga tcacgacagg
       61 ggaggccttc gtttccggcg cgaatgtgac gactgatccg gccgccgtcc gcagccgcat
      121 cggtgtcgtc ccgcagcggc caaaccccga ccgcagcctc tctgtcatcg aaaacctcgt
      181 cttccacgcc acatacttcg gcatcgggcg taccgaggcg gcgcggcgcg gcgagcagtt
      241 gctcgaacgg atggggatcg ccgacaagat gcacgcgaaa gtcgatcagc tctccggcgg
      301 ccagcagcaa cggctcatga tcgcccgcgc cctcagccac gatcccaagg tcgtctttct
      361 cgacgaaccg acggtcggac tcgatccgca ggcgcgcctc tcgctctggg agatcctgcg
      421 cgagctccac aatcaaggaa gaacgatcgt gatgtcgacg cactacatgg aggaggccga
      481 ccagctctgc aaccggttgg cgatcatcga ccagggaaaa ctgctggcgc tggacacccc
      541 cgcggtactc aaggcgaaag cgcccggcga cacgctcgtg gaggtgacgt tcgatgggga
      601 tgcatccgtc gccgcggatg ccgcccgagg catggaggga gtgtcgagcg cggaggcgcg
      661 cggaccgatt ctgcgcgttt tcagcaagcg cggcggcgag ttgatcccct cgctgatcaa
      721 cgcgggcgaa gcgcacgggc gggtcgttcg cgacatccaa ctgtcgcgtc ccagcctgga
      781 aacgctcttc atctcgctga ccggaaggaa actgacatga ccgtggagcc gctcgcgccc
      841 gtggcgccgt cccgcgtctt cttcgccatc ctgctgcgcg acctgacggt ggcacggcgg
      901 gagctcccgt actttctggt ccggacggcg cttcaaccca tcctcttcgt gaccgtgttc
      961 ggctttttgt tgccgcggat gggactgatt cctcacggct actcgacgac gatgcttccc
     1021 ggcgtggtgg ctctgagtct ggccctgtcc gccatccagt cggtgtctct gccgatgatg
     1081 caggacttcg gcttcacgaa ggagatcgaa gaccggctgc tgtcgccggt gccgattcac
     1141 ctcgtcgcgg tcgaaaaagt agtcggagga gtgctgcagg ggattgtggc ggctttgttc
     1201 gtactaccgg tggcgcgcct catcatgggg ccaattcccg gactgagctt ccgaaacctg
     1261 cctcttttgc tggcggtgac catcctcggc ggcgcggcct tctcggcgat gggcctctac
     1321 ctggggaccg gcatcgcgcc ccagcagatc ggtctgatgt tcagcgtgat cgtcgcgccg
     1381 atgatcatgt tcggatgcac gtactacccg tgggcaggac tttcgcacgt gccggtgctg
     1441 aagtggctgg tgctgatcaa cccgctggtt tacgtttctg aggggatgcg ggcggccctg
     1501 acgcccggga ttccgcacat gccgttgctc atgattctgg ccgcgctggt gcttctgacg
     1561 gtccttttca tggtcctggg gctgcgggcg ttcgacaagc gggcgatgag ttgaagctga
     1621 ggttaagcag cttttgctgg tttcgggaca caagtgcgcg taagatttct gtgcgactcc
     1681 ccttcgcgcc agaatcgtcg cattaatcgt ttcggttatt caatattgat ggactagaag
     1741 tcagggacag catagactgt tccaacgctg ttcatccaag catgaacaga gaaaaggaga
     1801 gagtgtgaca ccagctaaaa gcaatcgagg cagacctatc gttcacaccg agccatgggc
     1861 gaagatcacc gtcgtccttc tggatcggca tgtcgcgtat cttgatcgac tggccatcga
     1921 catccgtttg aggcatggca aagcgatcag ccgggcggaa atcatccgtg ggttgattga
     1981 agccgcgttt cagagcgggg tcgatctttc tgaggccgat tcgatcggaa cgctcgtcga
     2041 gctactgacg ggctcaccga tgaagacgcc gaagaagccg agctcgcgtt aggcgcgggg
     2101 cgcagttcgc ctcctgcttt tggaacgaag ctcgcctgcg gcgagccggg cgatctcgta
     2161 ctcactgcta gcggcggtga gatcgatgtt gctctgctca atgcggtcga ggatcgtccg
     2221 gattgcctgg ggccatccga aggccttcgg catcgcgggg ttgatttcgt ttgacatctt
     2281 cgccagaaac gaagcatgtc tcttcgagag gacaatggtc tctgtcgccg gcgccgtgcc
     2341 ggcgacactt cttcctgtgg gggtcagtct cataacctgg tactgatagg ggagtgtcgt
     2401 gagacgacca aatccctacc ccccacccca aaaagttttt tcctggccct tcgttggttg
     2461 gctgcgaccg gccgccgagt ctcaggcgga gaggggttcg gtctccggca ccagcatcac
     2521 aggcgtgtcg accttccgga tgatgtcctg cttcagcgtg ctgatctcgt gcgccccgac
     2581 gttgaggaac agcgcgtcgg cgtccacggt ctttgcgtaa cgggccagtt cggcctggac
     2641 atccttcccc tggaggacga ccggcaggac ccgcgctcca tcgaacgtgc cgcccgtcat
     2701 cgcgtcgaga agatcctgcg aggcaatccg ctccggctcc tcgcagacga tgatcagatg
     2761 gatctcggag ttttcacgcc gcgcgatcga
//
LOCUS       DATLVW010000823         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404761, whole genome shotgun sequence.
ACCESSION   DATLVW010000823 DATLVW010000000
VERSION     DATLVW010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404761"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VNN25_24065"
     CDS             complement(<1..488)
                     /locus_tag="VNN25_24065"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXH94673.1"
                     /translation="MLERNLTDGILTLRLSHGKASALDLDLCTALTKAFEEAAADDDV
                     GAVILTGTGSIFCAGVDLPRMINAGGDYVQLFVEALDAALRAVFVFPKPCVAALNGHA
                     IAGGAILAFACDHRLMSSGKIGVPEPLVGVPFPPLAMEIVRFAVPKQHLQPMVYFGRT
                     MDA"
     gene            787..>2056
                     /locus_tag="VNN25_24070"
     CDS             787..>2056
                     /locus_tag="VNN25_24070"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH94674.1"
                     /translation="MKQRFLMMSILVTFALFAATHAGAQQKTILFDAAHAQTAGNADW
                     TLDEDTCGTAQRFPTPDQSGITSGTAETYWNGAHSAMGVDLVKKGFHVESLPVGARIS
                     YGDGTNAQDLSHYNVFVMPEPNIALTSTEITAIRNFVFNGGGLFMISDHAGADRNSDG
                     IDAAGVFNQLMGSPSIFGITYNDNSADSTFGWFDDHPDHNYTTVTTSPIIFTGTFGVP
                     SPTKGLGLFGSSSMTLSGAAQGHVWKTTSTHDTSTGVTYATSTYGTGRIAAIADSSVT
                     EDATNNCGHSTFLGYNDPSYDNGLLVANGISWIANGSGGGDTTAPTVSITAPLNGATV
                     NGTVSVNATASDNVGVTKVEFYLDGVLTSTDTTSPYSWSWDTTSASNASHSLTAKAYD
                     AALNVGASTAVSVTVNNSGDTTAPTVSITAP"
BASE COUNT          445 a          725 c          546 g          340 t
ORIGIN      
        1 gcgtccatcg tccgcccgaa atagaccatc ggctgcaggt gctgcttcgg caccgcaaag
       61 cgcacgattt ccatcgctag cggagggaaa ggaacgccga caagcggctc cggaacccca
      121 atcttgcctg agctcatcag acggtgatcg caggcaaagg cgaggatcgc gccgcccgca
      181 atcgcgtgtc cgttgagcgc ggcgacgcac ggcttcggga agacgaacac cgcgcgcagc
      241 gccgcatcca gcgcctcgac gaagagctgc acgtagtcgc cgccggcgtt gatcatgcgc
      301 ggcagatcga cgcccgcgca gaagatcgat cccgtgccgg tgaggatgac cgcgccgaca
      361 tcatcatcgg ccgccgcttc ttcgaatgcc ttcgtcagcg cagtacacaa atccagatcg
      421 agcgcgctcg cttttccgtg cgaaaggcga agagtgagga tgccgtcggt gaggtttcgt
      481 tcgagcatgc ttgattctat gtggggagtg gggtgtgggg agtagggggt gggatttgaa
      541 caagagtttg ccgcgccgat tttttctccg acaaccgaca accgacaacc gacaaccaaa
      601 tccctcccac accccactcc ccaaaaccca ctccccacac acggcacttt acctgcaccg
      661 tacaaacaat ctccccacaa gttcccccac gtaagcccct tcttctggtc aagaactgca
      721 cactccccgg gtcaatcaac gctcaagtgg tcaacattca gaccacttgc aatcaatgga
      781 ggaagaatga aacaacgctt tctaatgatg tccatcctgg tcacgtttgc ccttttcgcc
      841 gcaacccacg ccggcgccca acagaagacc atcctcttcg acgccgccca cgcgcagacc
      901 gccggcaacg ccgactggac cctcgacgaa gacacgtgcg gcaccgcgca acgtttccca
      961 acgcctgacc agtcgggcat cacatcgggc accgccgaga cgtattggaa cggcgcccac
     1021 tccgcgatgg gcgtcgacct cgtgaagaaa ggctttcacg tggagagcct tcccgtcggc
     1081 gcgcgcatca gctacggcga cggcaccaac gcacaggacc tcagccacta caacgtcttc
     1141 gtcatgcccg agccgaacat cgccctcacg tccacggaga tcaccgccat ccgcaacttc
     1201 gtcttcaacg gcggcggact tttcatgatc tccgaccacg ccggcgccga ccggaatagt
     1261 gatggtatcg acgcggccgg cgtcttcaat cagctcatgg gcagcccgag catcttcggc
     1321 atcacctaca acgacaactc ggcagacagc accttcggct ggttcgacga tcatcccgat
     1381 cacaactaca cgaccgtcac cacgtcgccg atcatcttca ccggaacctt cggagttcct
     1441 tcgccgacga aaggcctcgg gctgttcggc tcctcatcga tgaccttgtc cggcgccgcg
     1501 cagggccacg tctggaaaac gacgtcgacg cacgacacgt ccacgggcgt gacctacgcg
     1561 acatcgacct acggcaccgg ccgcatcgcc gcaatcgccg actcctcggt caccgaggac
     1621 gccacgaaca actgcgggca ctcaaccttc ctcgggtaca acgatcccag ctacgacaac
     1681 ggtctgctcg tcgccaacgg catctcctgg atcgcgaatg gctcgggcgg cggcgacacg
     1741 acggctccaa ccgtttcgat cacggctccg ttgaacggcg ctacggtcaa cggaaccgtc
     1801 tcagtcaacg ccacggcgag cgacaacgtc ggcgtcacga aggtcgagtt ctatctcgac
     1861 ggcgtgctca catcgaccga tacaacgtcg ccctactcat ggagctggga tacgacctcg
     1921 gcgagcaacg catcgcactc attgaccgcg aaggcgtacg acgccgcgct caacgtcgga
     1981 gcatcgaccg cagtctccgt caccgtcaac aatagtggcg acaccactgc gcccactgtc
     2041 tcgatcaccg ctccgc
//
LOCUS       DATLVW010000824         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_323426, whole genome shotgun sequence.
ACCESSION   DATLVW010000824 DATLVW010000000
VERSION     DATLVW010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323426"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VNN25_24075"
     CDS             <1..346
                     /locus_tag="VNN25_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94675.1"
                     /translation="FFTETFGRMLGISDDLAKRMERTIERQFGIDWSHLRGTALAPQM
                     NAPLLVIHDEDDADVPARFGHDLADVWPDAVLMLTRNLGHRRILRDPTVIAAAIEFID
                     RQRPERIAANRR"
     gene            complement(518..3286)
                     /locus_tag="VNN25_24080"
     CDS             complement(518..3286)
                     /locus_tag="VNN25_24080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXH94676.1"
                     /translation="MITDPTPNVPERFRSAFRASTLATIQRQAGKFQLRGVLFFRGAS
                     TAHVIESERRTFQPVLVVDSRSVISARCACDPGMKTDDLCRHLGVLLARTADASGALP
                     FERFETSVWRAIGFAASAEGKAIAHESESDPRELLLRKSVLTEQEQALLKRGSGSARL
                     LWEASSWYRWAQARFADDPMGERAAIEFRDGSLMLGDIAVPAAAVEQIITSGIAARSG
                     FEVAPLSLTASLRIELTEDRALRFVPVLLRDVDEENVGRTLQSDGSSLAATGSRTGES
                     DLRKPIFVRAAVSQFGRFFLLDGAFASLRPATPMFVEQNLRTQASLFEERPPSGIALE
                     RETTIAESDVFAFLERHREELATMPAALAPDCVRNANPMRLEGDVAFDFGAPRGNALD
                     VEVVFETGVTAAEIVRARRDGIRALNRGDVWIDVRDPQFDCLDSVSMRDDGRLIVTPL
                     DVLRIRATLRGQAIFRGDPSGRDVFRMFDQVDVSSEAPSPSALGFDLYAYQRTGYHWL
                     WLLHHNGFGALLCDDMGLGKTHQAMALMGAVTAANPQTSILVVCPTSVLDHWREKLRC
                     YLPGQEVTLTSYGLVRSRLEQYRQTRFDLVVLDEMQMIKNADTATHQALAALNKGVAI
                     GLTGTPIENHEGELRTLLDFVVPGYLPGNIDDRATLRRLARPFVLRRTKGQVLTELPP
                     KIVDKRYCEMSEAQRSLYRAVIDSRAKPLQAQLRAGATISYVHVFAALNYLKQICNHP
                     ASAGGGFDGDAPSAKWDLFVELLDECTASGLKVVVFSQYLRMLEMIEQHLQTQMIGFA
                     SIKGSTKDRGEETARFRDDAACRVFTASLRAGGLGIDLTSASVVIHYDRWWNQAREDQ
                     ATDRVHRIGQNKGVQVIKMITRDSVEEKIDALIERKRRLANDIILDDDPSLVKQFTPE
                     ELEELLGG"
BASE COUNT          703 a         1132 c         1072 g          646 t
ORIGIN      
        1 gttcttcacc gagacattcg gtcggatgct aggcatttcc gacgatctcg cgaaacggat
       61 ggaacggacg atcgagcgcc aattcggcat cgactggtca cacctgcgcg gcaccgcctt
      121 ggcgcctcag atgaacgcac cactcctcgt cattcacgac gaagacgacg ccgatgttcc
      181 cgcgcgcttc ggccacgatc tcgccgacgt ctggcccgac gccgtcctca tgctcacccg
      241 caacctcggc catcgtcgca tcctgcgaga ccccaccgtg atcgcggcag cgatcgaatt
      301 tattgatcgc cagcggccag aacggattgc cgccaaccgt cgttagctgc ttcgccgcct
      361 tgccgccggt ccacggaatc gtgcaaagca ttgaatttga agcgtatgcg ctccgcgata
      421 gataggctga aagtatcagc ctcgaaaggc tgaaagaacc tgtacgaaaa tacagaaaga
      481 atctgtacag cacggctgat actttcagcc tcgagcatca cccgcccagc aactcctcca
      541 actcctccgg cgtgaactgc ttcacgagcg agggatcgtc gtcgaggatg atgtcgttgg
      601 cgaggcggcg tttgcgctcg atcagggcgt cgatcttttc ctccacgctg tcgcgcgtga
      661 tcatcttgat tacctgcacg cccttgttct ggccgatgcg gtgaacgcgg tcggtagcct
      721 ggtcctcgcg ggcctgattc caccagcggt catagtgaat gacgacggac gcgctggtga
      781 gatcgatgcc gaggccgccg gcgcgaaggc tggcggtgaa aacgcggcag gcggcatcgt
      841 cgcggaagcg cgcagtttct tcgccgcgat ccttcgtgct tcctttgatc gacgcgaatc
      901 cgatcatctg cgtttgaaga tgctgctcga tcatctccag catccgcagg tactggctga
      961 agacgacgac tttcagaccg ctggccgtgc attcatcgag cagctcgacg aagaggtccc
     1021 atttcgccga tggcgcatcg ccgtcgaatc cgccgcctgc cgatgcagga tggttgcaga
     1081 tctgcttgag gtagttgagc gcggcgaaaa cgtgaacgta cgaaatcgtc gcaccggcac
     1141 ggagctgcgc ctgcaagggc tttgcacggg agtcgatgac cgcgcggtag agtgaccgct
     1201 gcgcctccga catttcgcag tagcgcttgt cgacgatctt cggcggaagc tcggtcagca
     1261 cctggccttt cgtccgccgc aaaacgaacg gccgcgccag acggcgcagc gtggcgcgat
     1321 cgtcgatgtt gccgggcaga tagccgggga caacgaaatc gaggagcgtc cgcaactcgc
     1381 cttcgtggtt ctcgatcggc gttccggtca ggccgatggc cacgcctttg ttgagcgccg
     1441 ccagcgcctg atgcgtcgcc gtgtcggcgt tcttgatcat ctgcatctca tcgagcacga
     1501 ccagatcgaa acgcgtctgc cggtattgct cgaggcggct gcgaaccagg ccgtaggaag
     1561 tgagcgtgac ctcctggcct ggcagatagc acctcagctt ctcgcgccag tgatcgagca
     1621 ccgaggtcgg gcaaacgacc aggatcgacg tctgcggatt cgcggccgtg accgcgccca
     1681 tgagcgccat cgcctggtgc gtcttgccga ggcccatgtc atcgcagagc agggcgccga
     1741 aaccattgtg gtgcagcagc cagagccagt gatagccagt ccgttgatac gcgtaaagat
     1801 cgaatcccag cgcggacggc gacggtgctt cactggagac atcgacctga tcaaacatgc
     1861 ggaagacgtc gcggccggat ggatcgccac ggaagatcgc ctggccgcgc aacgtggcgc
     1921 ggatacgaag cacgtcgagc ggcgtcacga tcagccggcc gtcgtcgcgc atcgaaacgg
     1981 aatcgaggca gtcgaactgc ggatcgcgca cgtcgatcca gacgtcgccg cgattaaggg
     2041 cgcggatgcc gtcgcggcgg gcacggacga tctccgcggc ggtgacgccg gtctcgaaaa
     2101 cgacctcgac atcgagggcg ttgccgcgcg gcgcgccgaa atcgaatgcg acgtctccct
     2161 ccagtcgcat cggatttgcg ttgcgcacgc aatcgggggc gagggccgca ggcatcgtcg
     2221 cgagctcctc gcggtgccgc tccagaaacg caaagacgtc gctctcagca attgtcgtct
     2281 cgcgctcgag agcgatcccc gagggcgggc gctcttcgaa caacgatgcc tgcgtgcgca
     2341 agttctgctc gacgaacatt ggcgtcgccg gcctgagcga agcgaaggcg ccatcgagga
     2401 gaaagaaacg gccgaattgc gaaacagccg cgcggacgaa aataggctta cgtaggtcgg
     2461 actctccagt ccgacttcca gtcgccgcga gagaagaacc gtcggactgg agagtccgac
     2521 ctacgttttc ctcgtcaacg tcccgcagaa gaacaggaac gaatcgcaac gcgcgatctt
     2581 ctgtcagctc gatgcgcagc gacgctgtca gcgacagtgg cgcgacttcg aagccggaac
     2641 gcgccgcgat tccgctcgtg atgatttgtt cgactgcagc agcgggcacg gcgatgtccc
     2701 cgagcatgag cgatccatca cggaactcga tcgcagcccg ctcgcccatc gggtcatccg
     2761 caaatctcgc ttgcgcccag cgataccagg atgatgcctc ccacagcaga cgcgcgcttc
     2821 ccgatccgcg tttcaacaac gcctgctcct gctcggtgag cacgctcttg cgaagaagca
     2881 actcgcgcgg atcgctctcg ctttcgtgcg cgatcgcttt cccttccgcc gaagccgcaa
     2941 atccgatcgc gcgccagacg ctggtctcga atcgctcgaa cggcagcgcg cctgacgcat
     3001 ccgcggtgcg cgcgagcaag acgccgaggt ggcggcagag atcatccgtc ttcatgccgg
     3061 gatcgcacgc gcaacgcgcc gagatcacac tacgcgagtc gaccacgagg accggctgaa
     3121 acgttcgccg ctcactctcg atgacatgcg ccgtcgacgc accgcgaaag aagagcacac
     3181 cgcgcaactg aaacttgcct gcctgccgct ggatcgtggc cagcgtcgaa gcgcgaaagg
     3241 cggagcggaa tcgttccgga acgttgggag tgggatcggt gatcaacagg agagctcgtt
     3301 ttccaatctt agctgcttcc caattcgccg ctccgccatc cgatgatgag tccgctcatc
     3361 gatccgtcga gagacttcgt caaacggtta ctaatacagt tatcttgact ttactgaagt
     3421 gatgtacgta gtgtgcaagc aggtatccca catgacccgg atctcgaccc tcctcctcgg
     3481 ctgttttctc ttcgctacac ccatgttcgc ggactcagcg caggtcgacc tgcaagcgta
     3541 cgcggcgaac cct
//
LOCUS       DATLVW010000825         5452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_487664, whole genome shotgun sequence.
ACCESSION   DATLVW010000825 DATLVW010000000
VERSION     DATLVW010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487664"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..833)
                     /locus_tag="VNN25_24085"
     CDS             complement(105..833)
                     /locus_tag="VNN25_24085"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH94677.1"
                     /translation="MAPHETGFLLRSVVVGAHSYPYSVYVPQSYEPSKRWPVILFLHG
                     SGERGSDGLRSTQIGAAAAIRANPERVPAIVVFPQAPLETRWLGEPADAAMAALHAAM
                     AEFRGDPDRVYLTGLSMGGYGTYHLALAHPTTFAGLVIVCGGLLPHATTTAVQQSPLT
                     MNASDPYAFTAHAIRKFPIWICHGDADPVIPVEESRRMVALLRAEGAEVHYDEYAGVG
                     HNAWDRAYGDAEMWRWLFLQRRRK"
     gene            1002..1487
                     /locus_tag="VNN25_24090"
     CDS             1002..1487
                     /locus_tag="VNN25_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94678.1"
                     /translation="MSADSNQARTAPAHPFSALVKGGLVVGSLDIAYAIIFSAFRGFG
                     PIRILQSVAAGLLGKSSFQGGLPTATLGLALHYFIAFTIVIVYWMASRPLPILTAHPV
                     ICGAIYGLGVYFFMNYVVIPLSAANRPRFFLLLFICNLMVHAFLIGVPAALFARKARV
                     G"
     gene            1539..1907
                     /locus_tag="VNN25_24095"
     CDS             1539..1907
                     /locus_tag="VNN25_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HXH94679.1"
                     /translation="MHTAAVSAVVIVAFLHFVFLVLEMFLWATPRGRRIFALSPELAE
                     GSKKLAANQGLYNGFLAVGLLWGAFCPFPGNAGLRIQLFFLGCVIVAGVFGGLTTKRS
                     ILYLQALPGIIAFALVWMTR"
     gene            1925..2344
                     /locus_tag="VNN25_24100"
     CDS             1925..2344
                     /locus_tag="VNN25_24100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94680.1"
                     /translation="MNLDDVIAEMYASICFEEGQRPDWSRQAAIFAPGARLVRVTDEG
                     VFEFDLDSYRANLEAMIDSGAMTSFWEAEIWRETRQFGEVAHVLSAYETRRSRNGEVR
                     NRGINSIQLFRRDDRWSISAMLWRREGREVVIGDAAP"
     gene            complement(2481..3074)
                     /locus_tag="VNN25_24105"
     CDS             complement(2481..3074)
                     /locus_tag="VNN25_24105"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXH94681.1"
                     /translation="MRLLVFSWPYALFFWLVYLWAFAPEFAMIQRSTAPAAASGSKDS
                     GSLRFIVLGMWLGLLISFPIAFVKALRFPAAWNLAAFFIGTGLLVAGSLLRRHCWRML
                     GAHFTGDVRAREDQPVIDRGAYRWVRHPSYTGGILMFAGIGVALSNWASLAILVVVSV
                     AVYSYRIAVEEKALLSAIGEPYAAFMRSRKRFIPYVI"
     gene            3441..3695
                     /locus_tag="VNN25_24110"
     CDS             3441..3695
                     /locus_tag="VNN25_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HXH94682.1"
                     /translation="MALEIRPNCQNCGKPVAAGSAKVYICSFERTYCANCAENKLNMK
                     CSGCGGALVQRPVRSRALLAKYPAANRNGHKPLKAKKIKP"
     gene            complement(3708..4175)
                     /locus_tag="VNN25_24115"
     CDS             complement(3708..4175)
                     /locus_tag="VNN25_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94683.1"
                     /translation="MAAFEYRQAEEIRDTFARHGIRYLFIGKSGAILLGFPDTTQDAD
                     LFADKSAKNCSALVAALRELGFALSEQQASEILRGKDFVQLKNGPFDLDLIFAPDGID
                     RFSDAWGRRVEVNNFPVCHPDDIIASKVAANRTKDRESLPRLRAFRDYWKSRQ"
     gene            complement(4176..4307)
                     /locus_tag="VNN25_24120"
     CDS             complement(4176..4307)
                     /locus_tag="VNN25_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94684.1"
                     /translation="MKYAFIKTYKPVLDDAPFRAFDTMADYRAWCEANLPDWLGYGR"
     gene            complement(4434..>5452)
                     /locus_tag="VNN25_24125"
     CDS             complement(4434..>5452)
                     /locus_tag="VNN25_24125"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXH94685.1"
                     /translation="PEAGADNSAWITPLNRMNTRAIDRFGGTMLHTSRMNPQISKQEH
                     IPEYLEASKGPPDAGGRYDLTSAALRTINFLQLDGLVALGGDGTLTFARRLHNEGVPL
                     VAIPKTMDNDVFGTDYCLGFSTAVTRSVMFINDLRTGAGSHERFLVVELFGRYSGETC
                     LLASYLAGVDRAVIAEVPFDCEKLYQLLEADRMDNPSSYAVVAVSEGARLIGGGTIES
                     GEADAYGNRKLGGIGGIISDYLEKKSHDKAIYQRLAYLMRSGAPDSLDQLVANNYGSL
                     AADLMRRGDTGKMVAVVDGRYTAVPIEVGSAARKRVDVDRFYDVAHYRPKVADVMGMP
                     MFLH"
BASE COUNT         1063 a         1698 c         1654 g         1037 t
ORIGIN      
        1 gccggctccg gctcccgctc cgatgaagac gtgtcctgaa tgtctggaga tgattccggc
       61 cgcggcccgc cgatgccgcg cgtgcgcggc gtcgattgcc tgaactactt ccttcgccgt
      121 tgtaaaaaca gccagcgcca catctcggcg tcgccgtacg cgcgatccca cgcgttgtga
      181 ccgacgcccg catactcgtc gtaatgcacc tcggcgccct cagcccgcag cagcgccacc
      241 atccgccgcg actcctcgac agggatgacc ggatctgcgt cgccgtggca gatccagatc
      301 ggaaacttgc gaattgcatg cgctgtgaac gcgtagggat cggacgcgtt catcgtcaac
      361 ggcgattgct ggaccgccgt cgtcgttgcg tgcggcagta gacctccgca cacgatgacc
      421 agcccggcga aggtcgtggg atgcgctaac gccaggtggt acgttccgta gccgcccatc
      481 gacaaaccgg tgagatagac gcgatcggga tcgccgcgaa actcagccat cgcggcgtgg
      541 agtgcggcca tggcggcatc ggccggctcg ccgagccatc gcgtttcgag cggcgcctgc
      601 gggaagacga cgatggccgg aacccgctcc ggattggcgc ggatcgccgc cgccgcgccg
      661 atttgcgtcg agcgcaaacc gtcactaccc cgctcccccg agccatgcag aaaaagaatg
      721 accggccagc gcttcgacgg ctcgtacgat tgcgggacgt agaccgaata aggatagctg
      781 tgcgctccga cgacgacgga tcgaagcaaa aagccggttt cgtgaggtgc catggtgcgg
      841 caggacaaag gtgcagcgag cagaatcagg gacaacagtt tccagtgagc ctgcatggag
      901 cggcggaatg atacgacgcg actccgtcac cgcaagtttc ctatgagcga aggtttcgct
      961 gcgttagtct cgcggcttca caaaaccagg gggtgattac gatgtcagcg gattcgaatc
     1021 aggcgcgcac tgcgccggcc catcccttct ctgcgctcgt caaaggagga ctcgtcgtcg
     1081 gctccctcga catcgcgtac gccatcatct tctcggcctt ccgcggtttc ggaccgatcc
     1141 gcattctgca aagcgttgct gccggcctgc tcggcaaatc ctcatttcaa ggcggacttc
     1201 ccaccgccac cctaggcctg gcactccact acttcattgc cttcaccatc gtgatcgtct
     1261 actggatggc cagccgtccg ttgccgatcc tgacggcgca cccggtcatc tgcggcgcga
     1321 tttacgggtt gggcgtctac ttcttcatga actacgttgt gatcccgctc tccgccgcca
     1381 accgcccgcg ctttttcctg ctgctgttca tctgcaatct catggttcac gcgttcctga
     1441 tcggtgttcc ggcggcgctc ttcgcgcgga aagcccgcgt cggctaaccg cagtgcggct
     1501 ccgtggtagg ctcggcagca atccaattcg gagcacacat gcacactgcc gcggtcagcg
     1561 ccgtcgtcat cgtcgcgttc cttcacttcg tgtttctggt gctcgaaatg tttctctggg
     1621 cgacgccccg aggcaggcgc attttcgcgc tgtcgcccga gctcgcggaa ggctcgaaga
     1681 agctggcggc caatcagggg ctgtacaacg ggttccttgc ggttggcctc ttatggggtg
     1741 cgttctgtcc attcccagga aacgccggcc ttcgcatcca actcttcttc ctcgggtgcg
     1801 tcatcgtcgc cggagtcttc ggcggcctga ccaccaaacg ctcgatcctt tatctgcagg
     1861 cgctgcccgg catcattgcg tttgctctgg tttggatgac gcgttgagca caacatcgga
     1921 tcctatgaat ctcgacgatg tcatcgccga aatgtacgcc tcgatctgtt tcgaggaggg
     1981 ccagcgtcct gactggtcgc gtcaggcggc aatcttcgct ccgggcgcgc ggctggtacg
     2041 cgtcacggac gaaggcgtct tcgaattcga tctcgactct taccgcgcca atctcgaagc
     2101 aatgatcgac tcgggcgcga tgaccagctt ctgggaagcc gagatctggc gcgagacccg
     2161 gcaattcggc gaggtcgcgc acgtcctcag cgcctacgag acccgccgct cgcgcaatgg
     2221 cgaggtccgg aaccgcggca tcaacagcat ccaactcttc cgccgcgacg atcgctggtc
     2281 gatcagcgcg atgctctggc gacgggaagg gcgagaggtt gtgatcggcg acgcggcgcc
     2341 gtgaagacgg agatcggcga tcagagataa gagatcaggg gttttaatct ctgatcgctg
     2401 atcttctgat ctcttttgag cgaccagaga caagagatca ggccttctaa tctctgatct
     2461 ctgatcctct tatctctgct ctaaatcacg tacgggatga accgcttcct cgatcgcatg
     2521 aatgccgcgt acggctctcc gatagcgctg agcagcgctt tctcttcgac cgcgatgcga
     2581 tatgagtaaa cggccacgga gacgacgaca aggatggcga gactggccca gttggaaagc
     2641 gcgactccga tgccggcgaa catcaggatg ccgccggtgt acgacggatg gcgcacccac
     2701 cgataagcgc cgcggtcgat caccggctga tcctcgcgcg cgcgcacgtc gcctgtgaag
     2761 tgggcgccaa gcatgcgcca gcagtggcgg cgtagaagcg agccagcgac cagcaatccc
     2821 gttccgatga agaacgccgc cagattccac gccgccggaa agcgaagcgc cttcacgaat
     2881 gcgatcggaa acgagatcag aaggccgagc cacatcccca gcacgatgaa tcgcaacgag
     2941 ccggagtctt tcgatccgct cgccgcggcc ggtgcggtcg agcgttggat catcgcaaac
     3001 tcaggcgcga acgcccacaa gtagacgagc cagaagaaaa gcgcgtaggg ccaggagaag
     3061 accagtaatc gcaagcgcag aaaatactac ggcgccgggt gggaaggaag tcttccgggt
     3121 gtgggggcgt cgccctctca cgcgaagacg cgaaggcgcg aagagaaact ggaccaaaac
     3181 gaactccttc gcgtcttcgc gccttcgcgt gaaaaatccg atattcgctg acgctcgcga
     3241 aaaactcatt gtagcaatga ggctcgccgc caccttccgg cagccgtcaa gctccccgtt
     3301 cgcccgcgca atgaagccat cagcgctcgt tccgacaaaa acagacaggg tcattgtggc
     3361 accaagcgaa ccgtcaacta gaggagtcat tcgttgtatc ctgagacaag ctctcaactc
     3421 atacaaccag gaggccaacg atggcactcg aaattcgccc caactgccag aactgtggca
     3481 aaccggtggc ggccggatcc gcgaaggtct acatctgctc gttcgagcgt acctattgtg
     3541 ctaactgtgc cgagaataag ctcaacatga agtgttcggg atgcggtggc gccctggttc
     3601 agcggcccgt tcgcagcaga gcgcttctgg ccaaataccc cgccgcaaac agaaacggcc
     3661 acaagcctct gaaagcgaag aaaatcaagc cctgacgcgc cgggcgctta ttggcggctt
     3721 ttccagtagt cgcggaacgc gcgcaggcgt gggagcgact cgcgatcctt ggtccggttc
     3781 gccgcaacct tgctcgcgat gatgtcgtcg gggtggcaga ccggaaagtt gttcacttcg
     3841 acccgacgcc cccatgcatc gctgaatcga tcgataccat ctggagcgaa gatgaggtcg
     3901 agatcgaagg gaccattctt cagttgtacg aagtccttac cccgcagaat ctcagaagcc
     3961 tgctgttcgc taagtgcgaa tccaagctcg cgcagcgcag caaccagcgc ggaacagttc
     4021 ttcgccgact tatcggcaaa caaatccgcg tcctgcgtcg tgtcaggaaa gccgaggaga
     4081 atcgcaccgg acttgccgat gaagagatag cggatgccat ggcgggcgaa agtgtcgcgg
     4141 atctcctcgg cttgccgata ttcgaacgcg gccatctatc tcccgtatcc gagccaatcc
     4201 gggaggttgg cttcgcacca ggcgcggtaa tcggccatcg tgtcgaatgc ccggaatggc
     4261 gcgtcgtcga gcaccggctt gtaggtcttg atgaacgcgt atttcatgcg ctgttctaat
     4321 gggcggcgtg ccgcggcctc aaattcttcc agccattgtt ggcgttcaga cgctgacggt
     4381 tcatccatga ttggaattgt agccgggcct gaagttccga agcacttcgc cggtcaatgc
     4441 aggaacatcg gcatgcccat cacgtccgca acctttggcc ggtagtgggc gacgtcgtag
     4501 aagcggtcga cgtcgacgcg tttgcgggcg gcgctgccga cttcgatcgg gacggcggtg
     4561 tagcgtccat cgaccacggc gaccatcttg cccgtgtcgc cgcgccgcat caggtcggcg
     4621 gccaggctcc cgtaattgtt ggcgacgagt tgatcgagcg agtcgggagc accgctgcgc
     4681 atcagatagg ccaagcgttg atagatcgcc ttgtcgtgcg acttcttctc gagatagtcg
     4741 ctgatgatgc cgccgatgcc gccgagtttg cgattgccgt aagcgtccgc ttcgccgctc
     4801 tcgatcgtgc cgccgccgat gagccgcgcc ccttccgaca ccgccacgac cgcgtaactg
     4861 cttgggttgt ccattcgatc ggcttcgagc aactggtaga gcttctcgca atcgaacggc
     4921 acctcggcga tgacagcgcg atcgacgccg gcaagatacg acgccagcag gcatgtctca
     4981 cccgaatagc ggccgaacag ctcgacgacg agaaaccgct cgtgcgagcc ggcgcccgtg
     5041 cggagatcgt tgatgaacat caccgagcgc gtcaccgcgg ttgagaagcc gaggcagtag
     5101 tcggtgccga agacgtcgtt gtccatcgtc ttggggatgg ccacgagcgg cacgccctcg
     5161 ttgtgaagac ggcgcgcgaa cgtgagcgtg ccgtcgccac cgagggcgac gagaccatcg
     5221 agttggagga agttgatcgt gcggagagcc gcgctcgtaa ggtcgtagcg gccgcctgcg
     5281 tccggcggac ccttcgacgc ttccagatac tcgggaatgt gttcctgttt gctgatctgc
     5341 gggttcatgc gcgacgtgtg caacatcgtg ccgccgaagc ggtcgatggc gcgcgtgttc
     5401 atccggttga gcggagtgat ccacgccgag ttgtccgcgc ccgcctcggg ga
//
LOCUS       DATLVW010000826        12094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404846, whole genome shotgun sequence.
ACCESSION   DATLVW010000826 DATLVW010000000
VERSION     DATLVW010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404846"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1099
                     /locus_tag="VNN25_24130"
     CDS             107..1099
                     /locus_tag="VNN25_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94686.1"
                     /translation="MTVLLLAAGHSHHLSDGSTLQHHKPLLIARAGSCSGDCPTRDSA
                     VAQFVYADQSLSTAQDSLEAAASGGGAWALSGSDISLQKASSSDPDLPALTVTSNARA
                     SNTLIPSTSAEREDISWIADHKQVCPSGCALDPAVLASIPPSSVVARFHLRSGRVFTQ
                     SITRIGANVTPVHFSRLDGSGSASAYTQAIASWVGAEIQLSGTGIEIVESKFNGDPGR
                     SMTLTPDANNSMEIAVLNLPPFVPPASANNNAPQVGKHFEAYYDLMQNPPASESRLVP
                     FAGAASGSASYPTIDWSTIHPQSSVYSQLLNTLRLDIGRSAYERVLCPPDYIEP"
     gene            1156..1560
                     /locus_tag="VNN25_24135"
     CDS             1156..1560
                     /locus_tag="VNN25_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94687.1"
                     /translation="MDVFGGLTRAVAVAVFVAACAPPRQPSPREERIAAASLLTRRCA
                     ASRLANWNIRGVAAGADCSILLVDTSMVLDDSLVEAIHYGTGPYAVVDGGVRQFTRLR
                     AFRGVAYRDRIGRVRSYGNLTQREAEELQPCR"
     gene            1551..3788
                     /locus_tag="VNN25_24140"
     CDS             1551..3788
                     /locus_tag="VNN25_24140"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXH94688.1"
                     /translation="MPVRLASIVVLAMALVAPLAIAAPHRSALLIGINDYTASRLTTI
                     RAPAPDRDWPNLSGAVNDTIAMRELLVLLRGFDARDIVTLNDQSATRSAILAAANALA
                     ASASRDDVVFFFYAGHGSQVKNSRSDEPDKLDESIIPADSRRGAADIRDKELRRIFNA
                     ILDRGAHLTVIIDACHSGSAARGLLTLARPRGIHPDLRDVADGGDYGPRPENRGALVI
                     SAAHDDEEAREVRDDQQTMHGAFTWAWMRAMRDAATASPSSIDEPAMETFLRAQARLR
                     AAMPFQNPVLAGNAAQRRMPFLGTHDTTRAARAMFVIEHVRDGTAVISGGWVQGITIG
                     SELRAAGDRSQPPLTVKALLGLGRCEAHVPATAHVRSGELLELTGWAAPPPRPLRVAI
                     PLSDGDIAGFARRAAKIAAERDVKWINDPTVRTPEHLLRRAGSRWELLDASGHASSYG
                     SDDVALLALGRLPGGTSLFVQLPATGTIAAQIAYAAVIDVTGSAEDADYVLAGRFVRH
                     RVEYAWVRPNAARSDRRASGLPLRTTWIGDAGGDLGSVLLNRALRLHKIEAWSLLESP
                     PEARWPYRLLLRGERDQQLVADGGSVKGHNAYTLELRAASPVAARTRRRHVYVFSIDS
                     YGQSTLLFPVSGSVENHFPLAGAAAPQVIPLGAKFEIAPPYGVDTYMLLATDEPFANP
                     WILQWDGVRGPMPPTRTALERLVALTSSSNRGTPVLTPATWSIERIVIQSVRPSAGPR
                     RSRKR"
     gene            3921..5018
                     /locus_tag="VNN25_24145"
     CDS             3921..5018
                     /locus_tag="VNN25_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HXH94689.1"
                     /translation="MGFYLHKSVAVGPFRFNLSGSGVGMSVGVRGLRVGSGPRGNYVR
                     MGRGGVYYQHTFHSLAAPRTTPSVTPRMLPSPHVPAGTHAPLQEIASSNAAQIADSTS
                     EQLLSELREKRAAMAVTPLAIVGSILLLVLALFLLASAALPGWELLPLAIAAVVAIAA
                     AHRRDLLRKTTVILYDFTTEVEESFRTFTQWADALASCGRTWHVAAEGRVYDRKYHAG
                     ATSLLQRNATTVRSAPPPFVRTNVPVLSVAAGRRTLYFLPDHLLVYDGPTIGAVSYGS
                     VDLAVARQRFIEDGGVPSDATVVDYTWRYVNKSGGPDRRFNNNPRIPICLYDELHFRT
                     ASGLNEVLQVSRSGTGEGFAAAVRHLGTIGT"
     gene            complement(5184..5849)
                     /locus_tag="VNN25_24150"
     CDS             complement(5184..5849)
                     /locus_tag="VNN25_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94690.1"
                     /translation="MIRVIAIVSPSDIRDAIVGPPLLRNLSHCKSSEVIEEVPVDEDT
                     NAIVPLESSAPSETDAAQELAVHPPEALAAGVAKVLDLIAERLELEIPHPSTARRVRG
                     ARTVSREFVLSLMAAADRRPDLSFFARFDSAEARSVLESEDAYRLTAERTAMFLASLK
                     YTIEARWARVVADAMLAFTMATIFAKDPSNADLAAEVENMRRHLGRKGVRKKKAKKGE
                     APE"
     gene            complement(6405..9212)
                     /locus_tag="VNN25_24155"
     CDS             complement(6405..9212)
                     /locus_tag="VNN25_24155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94691.1"
                     /translation="MLKPVTILALDDDAAALAEAVQRRVAAANGLDDLVQFRLAGALT
                     DAINSIHVQRQRPDSPLRSRDDISARELVLVVVSAAGAARTTLLDTIATIRTTYEMRR
                     LASYFSIEVLCLLPEVTGATNDAGYAAAYGVLKTLSGADPKPFDEVWLLDATNGARVK
                     FGTLVTSLDTYADAVAGALTFEPEMSGALPGINPRGMPPAFSSFGCAELVFPREAAQQ
                     RLETRFASELFVALCGSGNPVAHSSSALAAKQFIVGEDFAVPLSRIGLDAGQSLFRRF
                     QPKSVVGEKMRSADELIAGARAELQQHRDSVHLKNLRALEEQREKTASAAAELLSHVV
                     DETLDRDDYASAAAFTGALIDPLPDVRDDADTAPRNVVTEIRAATAALDARIRFTPNT
                     AASDTARKRVRELSAMLRDQKLVAETVAADSAAEQIEELDRERESLLHQLPEVIFAEE
                     AENNAARNAAREAEAARLATESEAKEQQLRELFAQKPRAEQALREALEARRIWLWQKL
                     GWAAGGVVAIYAIPYAFDILRQNLRQVTWTAVVFLILFGAFAAFKYATSIAPRLREAR
                     ETLDRLVAQIDATDKAKNAAYNDELQFEHDMAHRRATLAVHRQVREAAQETLDALRAR
                     RDDLERLGAAPAAAPITAAGLAISIIDESEVDAWYVRTADDRKPFVRDFPISRSASRH
                     IGLDELRERVATYASSAFASFRALTMATAATSITTSANLARRLKRLVDLSAPLIELRD
                     DDFEAQKAIQRDATLWTDESDALWLRQLRERFTEAQVKASPDPLRVHVMTRTLHYPAF
                     VIGQIDYMRTQYDAAANREAESFPDLLPSDLAVTGAVRAAYEQVLLGRAAGIIELREN
                     GALAISTAILGDTYLAAAKRLAASDAAALRHTIADALAPRLSDAAEVARDLHSLVDGN
                     TLTQLDRGIIEALLKRYDTIM"
     gene            complement(9222..11969)
                     /locus_tag="VNN25_24160"
     CDS             complement(9222..11969)
                     /locus_tag="VNN25_24160"
                     /inference="COORDINATES: protein motif:HMM:NF025181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HXH94692.1"
                     /translation="MKNNVVFRPTVVIGLGGTGYGTLLKLKKRFIDAYGFVPPIISFL
                     AIDTTENAEGGAAIEKAQAEGTLDHNEIFVLQVGNPAALVNGTNAHIDEWWPSQIPTQ
                     SIIAGAGQVRARGRLALFARANDVYGRIRRAVDDVRNIRNQKQMYADQFQVSARAGVE
                     VQIVGSVAGGTGSGTFLDIAFMARDIIGSDEQSNFTGVLLLPGIFTRYAGVALVKPNA
                     YGALKEVEYLSTMKGGFQIDYGVRRVDVTRPPFDLVYLIDNINEQGRVVSEQQDLYGL
                     IADGMYVQIGSQIGADSANAVDNIKTQIATAGRVKGRSPQYCSFGVASLVMPSFDPLM
                     WEDAKRLVNDELLNGTVVDAEIEEDVQRFIDANRLREDNADQVIENLSQREGGGQLRF
                     PMSLQQLKFDNKAPTEIKNLHTVHRSRAERQIAQAITKNFEALRDQSVAAINTWWDAA
                     VNRNNGFTRAERFFEKLQAKLEWYRNMMDGEAKEERAKLKALSFKPAEDQIGEAVGAF
                     FKREGRIKTACEVYGGLVDRETELTIKIARLEKAAELYSVLHAHTQSILQACYRIREN
                     LNATRKQFEQQYLDATNPRGASRLFEQVVSFDPGQHRPKVTGEDFVKWYADHHNGSLA
                     QWSTLRADEVYADVREFVRKCYEPLTSLTMDDILGRLSPEAVSQELKRVDNLAVPLWR
                     YDAGKIPVVNQSVINEFYHYGVGDADTTMLKESRYETGVPKGSARPSMVSTHDAQRVL
                     LFKVKTGIPLFALADIDDMERAYLDPNKVISNHVDKRWQDLPNLIPRSGDGEALRWFA
                     LAQAPEPFAIIKRDGAWYSMKSKSGRRVDNNLLRLGQGRVPAYAAFEKNRELVQEAEE
                     AIDAVVTREGAVRVASILKEYTISLSQQMSGVSDAAVKDQVEHEVIAIEDYVRRMETL
                     S"
BASE COUNT         1986 a         4007 c         3861 g         2240 t
ORIGIN      
        1 catcgatagg agatatcacg aaggagaaac caccttgacg agcttcattc taaaaatctt
       61 tttcagcggt ctcatggtct ttacacccag ccaggacggt cacgaaatga ccgtcctcct
      121 cctcgcggcc ggccacagtc atcacctctc ggacggcagc acgctccagc atcacaaacc
      181 gctcctcatc gcgcgcgccg gctcttgcag cggcgattgc ccgacccgtg acagcgccgt
      241 ggcacagttt gtctatgccg atcaatctct gtcgacggcg caggactcgc tcgaggccgc
      301 ggccagcggc ggcggggcct gggcactttc cggttcggac atctcgttgc aaaaggcaag
      361 cagcagcgat cctgacttgc ccgcgctcac ggtcacctcg aacgctcgcg catccaacac
      421 gctgatcccg tcgacgtccg ccgagcgcga agacatcagt tggatcgccg atcacaaaca
      481 ggtctgcccc tcgggctgcg cgctcgatcc cgcggtgctg gcctcgattc cgccatcgtc
      541 cgtcgtggcc cgcttccatc tgcgcagcgg acgcgtgttc acgcagtcga tcacgcggat
      601 cggcgcaaac gtcacgccgg ttcatttctc gcgccttgac ggctcaggca gcgcctcggc
      661 gtacacacag gcgatcgcct cgtgggtggg cgcggagatc caactctcgg gcaccggcat
      721 cgagatcgtt gagagcaaat tcaacggcga tcccggccgg tcgatgacgc tcacgccgga
      781 cgcgaacaac tcgatggaga tcgccgtgct caatctcccg ccgttcgtac cgcccgcgtc
      841 ggcgaacaac aacgcgccgc aggtggggaa gcacttcgag gcttactacg acctcatgca
      901 gaacccgccg gcgagcgaga gccgtctcgt tccgttcgcc ggcgcagcga gcggttcggc
      961 aagctatccg acgatcgatt ggtcgacgat ccatccgcag tcgtcggtct attcgcagtt
     1021 gctcaacacg cttcggctgg acattggacg ctcggcctat gagcgcgtgc tctgtcctcc
     1081 ggactacatc gagccgtaaa catttcctgg aacggttcgc cgcacgcggc gaaccgttcc
     1141 gctcgggaaa aggtcatgga cgtcttcgga ggattgacta gagcggtagc cgttgcggta
     1201 ttcgtagccg catgcgcacc accgcggcag ccgtcgccgc gcgaagagcg cattgccgcc
     1261 gcttcgctgc tcacccggcg gtgcgcggct tcgcggctcg cgaattggaa catccgcggc
     1321 gttgctgccg gtgctgactg cagcatcctc ctcgtcgaca catcgatggt gctcgacgat
     1381 tctctcgtgg aagcgattca ctacggcacc gggccgtacg ctgtcgtcga cggcggcgtg
     1441 cgccagttca cccgtctgcg cgctttccgc ggtgttgcct accgcgaccg catcggccgc
     1501 gttcgaagct acggaaatct tacgcaacgc gaagccgagg agctgcagcc gtgccggtga
     1561 gactggcgag catcgttgtg ctcgctatgg cgcttgttgc tccgctggcc atcgccgcgc
     1621 cgcatcgatc ggctctgctc atcggcatca acgactacac agcgtcgcgg ctgacgacga
     1681 tccgcgctcc cgcgccggac cgcgactggc cgaatctctc gggtgcggtg aacgacacga
     1741 ttgcgatgcg tgagctcctc gtgctcctgc gcggcttcga cgcgcgcgac atcgtcacgc
     1801 tcaacgacca gtcggcgacg cggagcgcaa tcctcgccgc tgcaaatgct ctcgctgcgt
     1861 cggcttcgcg ggacgacgtc gtcttcttct tctacgccgg ccacggatcg caggtgaaga
     1921 actcgagatc agacgagccg gacaaactcg acgaatcgat catcccggcg gattcgcgtc
     1981 gcggcgcggc cgacatccgc gacaaggagc tccggcgcat cttcaacgcc attctcgatc
     2041 gcggcgcgca cctcaccgtc atcatcgacg cctgtcacag cggctccgcc gcgcgcgggc
     2101 ttctcacgct ggcgcggccg cgcggcattc atcccgatct gcgcgacgtc gccgacggcg
     2161 gtgactacgg tccgcgtccg gagaaccgcg gcgcgctcgt catcagcgcc gcgcacgacg
     2221 acgaagaggc tcgtgaggtg cgcgacgacc agcaaacgat gcacggcgca ttcacctggg
     2281 cctggatgcg ggcgatgcgc gacgcggcga cagcatcgcc atcgagcata gatgagccgg
     2341 cgatggagac gttcctgcga gcccaggcgc ggctgcgcgc ggcgatgccg ttccagaatc
     2401 cggttctggc cggtaatgcc gcgcagcggc gtatgccctt cctcggcaca cacgatacga
     2461 ctcgggccgc acgcgccatg tttgtcatcg aacacgtccg cgacggcacg gctgtaattt
     2521 ccggcggatg ggtgcagggc atcaccatcg gaagcgagct gcgagccgcc ggcgaccgct
     2581 cgcaaccgcc cctcaccgtc aaagccctcc tcgggctcgg acgctgcgaa gcgcacgttc
     2641 ccgccacggc ccacgtccgc agcggagagc tgctcgagct caccggctgg gccgctccgc
     2701 cccctcgtcc gcttcgcgtc gcgatcccgc tcagcgacgg cgacatcgcc ggcttcgcgc
     2761 gccgcgccgc gaagattgcg gcggaacgtg acgtgaaatg gatcaacgat ccaactgtgc
     2821 gcacgccgga gcatctgtta cgccgcgccg gttcgcgatg ggagctcctc gatgcctccg
     2881 gacacgccag ctcatacgga tccgacgatg tggcgctact cgcgctcggg cggctccctg
     2941 ggggaacatc actgttcgtc caactcccgg cgaccgggac gatcgccgcg cagatcgcat
     3001 acgccgccgt catcgacgtg accggcagtg ccgaagatgc cgactacgtc ctggccggcc
     3061 gtttcgttcg tcatcgggtc gagtacgcgt gggtgcgccc gaatgcagcg cggagcgacc
     3121 ggcgcgcgtc cggcctgccg ctgcggacca catggatcgg cgatgctggg ggcgacctcg
     3181 gctccgtact tctcaaccgc gccctgcggc tgcacaagat cgaggcctgg agtctgctcg
     3241 aatcgccacc cgaggcgcgc tggccgtacc ggcttctgct gcgaggagaa cgcgatcagc
     3301 aactcgtcgc cgatggcggc agcgtgaaag gccacaatgc atacaccctc gagcttcgcg
     3361 ccgcatcgcc ggttgccgcg cgaacccgcc gccgccatgt ctacgtcttt tcgatcgaca
     3421 gttacggcca gagcacgctt ctttttcccg tgagcggctc ggtcgagaat catttccctc
     3481 tagccggagc cgcagcgccg caggtgatcc cgcttggagc gaagttcgag attgcgccgc
     3541 cgtatggtgt cgacacgtac atgcttctcg cgacagacga gccgttcgca aatccgtgga
     3601 tcctgcagtg ggacggcgtc cgtggtccga tgccgccaac gaggacggcg ctcgaacgtc
     3661 tggtcgcgct caccagctcg agcaaccgcg gcacgcccgt cctcacgccc gcaacgtggt
     3721 cgatcgaacg gatcgtgatc cagtcggtcc ggccatcggc cggcccgcgg cgctcccgga
     3781 aacgctgaca ccgccacaaa agcgctgcat cggtgtcttt gcgagtgcgg cctcctcgcg
     3841 gacggcggct ggcggcaggc acgtgcagcc ggctgcaccg ccgtgaagga ggtcgcactc
     3901 gtgcgtctcg cggaggatga atgggcttct acctgcacaa gagcgtcgca gtcggaccgt
     3961 tccgcttcaa cctatccggc tcaggcgtcg ggatgtcggt cggcgttcgc ggactccgcg
     4021 tcggcagcgg gccgcgcggc aactacgttc gcatgggccg tggcggcgtc tactaccaac
     4081 acacctttca ttcgctcgcc gctccgcgca caacgccgtc agtcacgcca cgcatgctgc
     4141 cgtcgccaca cgttccggct ggcacgcatg cgccgctgca ggagatcgcg tcatcgaatg
     4201 ccgcgcagat cgccgactcc acttctgaac aattgctctc cgagcttcgc gagaaacgcg
     4261 cggcgatggc cgttacgccg ctcgccatcg tcggatcgat ccttcttctg gtcctcgctc
     4321 tttttctgct cgcttcggcg gccctgccag gttgggaatt gctcccactc gcaatcgccg
     4381 cggtcgtcgc gatcgccgct gcgcaccgtc gcgacctgct gcgcaagacg acggtaatcc
     4441 tctacgactt cacgactgag gtggaggagt cgttccgaac gtttacgcaa tgggccgacg
     4501 cgctcgcgtc gtgcggccgc acatggcacg ttgcggccga gggacgcgtt tacgaccgga
     4561 agtatcacgc cggcgcgacg tcgctcctgc aacgcaacgc gacgacggtg cgcagcgccc
     4621 cgccgccgtt cgtgcggacc aatgtgccgg tcctgagcgt cgccgccggc cggcggacgc
     4681 tgtacttcct gccggatcat cttctcgtct acgacgggcc gacgattggc gccgtcagtt
     4741 acggcagtgt tgatctcgcc gttgcgcggc agcgcttcat cgaagacggc ggcgtgccga
     4801 gcgacgccac cgtagtcgac tacacatggc gttacgtgaa caagagcggc ggtcctgacc
     4861 gccgtttcaa caacaacccg cgcattccga tctgcctgta cgacgagttg cactttcgca
     4921 ccgcatcagg cctcaacgaa gttctccagg tctcgcgcag cggcaccggc gaagggtttg
     4981 cggctgcggt ccggcacctc gggaccatcg gcacgtgatg cgctagtgag catctgctcc
     5041 tccagcggag tctcgatacc gcagtacacc gctctctcgc gattgcgaat caagcccctt
     5101 cgtctggaag accagcggcg ggttagcaac actgagtcgg ctcaaaatcc gtggcctccg
     5161 gtgtgacaac ccgcgttcta ccctcactcc ggagcctcgc ccttcttcgc cttcttcttc
     5221 cgcacgccct tgcggcccag gtgccgccgc atgttttcga cctcggcggc cagatccgcg
     5281 ttactcggat ccttggcgaa gatggttgcc atcgtgaacg cgagcatcgc gtcggccacg
     5341 actctggccc agcgggcctc gatcgtgtat ttcaggctgg ccaggaacat ggcggtgcgc
     5401 tcggccgtga gccgatacgc gtcttcggat tccagcacgg accgcgcctc ggcactgtcg
     5461 aatcgtgcga aaaaagaaag gtccggccgg cggtccgccg cggccatcag cgacagcacg
     5521 aactcccgcg agaccgtccg cgcacctcga acacggcgcg ccgtcgacgg gtggggaatc
     5581 tcgagctcca ggcgttccgc gatcaggtcc agcaccttcg caacccctgc cgccagtgcc
     5641 tcggggggat ggacggccag ctcctgtgcg gcgtccgttt cggacggcgc actgctttcc
     5701 agcgggacga tcgcgttcgt gtcttcgtcc acaggtacct cctcgattac ctctgacgat
     5761 ttgcagtgcg aaaggttacg cagcagaggc gggccaacaa tggcgtcgcg aatgtcggac
     5821 ggcgatacga tcgcgatgac gcggatcacc tcgcgagacc atttcgtcga attgaatatc
     5881 tgacgctcag acattcgcta caacgcggaa gtcattcaga gctcgctgct ggtcatgaat
     5941 atctgagcgt cagacattcg caacgtccaa ggacccgcga atgtctaacg gccagacgtt
     6001 cgcgatgacg cggtagcttc gaatgtctga cggccagacg ttcgcgatga cgcggtcacc
     6061 tcgcgagacg atttcgtaga atcgaatatc tgacgctcag accttcgata cgacgcggaa
     6121 gtcattcaaa gcccgccgcg agtcatgaac gtcggagcgc cggacactcg cagagccgcc
     6181 ggggccgcga acgtccggca cgcaaacact cgcggtgccg cggcagcggt tcgaagctgg
     6241 cttcgagccg tgaatatccg accgcatgac ggcttctgct gaatgatatc ggcggcgcgc
     6301 cggcgggact cgaacctgca acctctgcgt cgatccagcc ggaatattct gttgactcga
     6361 caagaagctt cgtcagggaa cgagcggccg caagagacga ggccctacat gatcgtgtcg
     6421 tagcgcttga gaagcgcctc gatgatgccg cgatcgagct gcgtgagcgt gttgccatcg
     6481 acgagcgagt ggagatccct cgcgacctcg gcggcatcgg acagccgagg cgcgagcgcg
     6541 tcggcgatcg tgtggcgcag cgcggcggca tcggatgctg ccaggcgttt tgccgcggcg
     6601 agatacgtgt cgccgaggat tgcggtgctg atggccagcg cgccgttctc gcgtagctcg
     6661 atgatgccgg cggcgcggcc gagcaacacc tgctcgtacg cggcgcggac cgcgccggtc
     6721 accgcgaggt ccgatggcag caggtcgggg aatgattcgg cttcgcgatt cgctgccgcg
     6781 tcgtactgcg ttcgcatgta gtcgatctgg ccgatgacga acgccgggta gtgcagcgtg
     6841 cgcgtcatga cgtgcacccg taacgggtca ggcgacgcct tcacctgcgc ctcggtgaac
     6901 cgctcgcgga gctggcgcaa ccagagggcg tcgctctcgt ccgtccacag cgtggcatcg
     6961 cgctggattg ccttctgcgc ttcgaaatcg tcatcacgca attcgatcag cggcgccgaa
     7021 agatcgacca accgcttgag gcgccgggcg aggttcgcac tcgtagtgat gctcgttgcc
     7081 gccgttgcca tcgtcaaggc gcggaacgac gcgaacgccg acgatgcgta tgtcgcgacg
     7141 cgctcgcgca gctcatcgag gccgatgtgg cgcgatgccg agcggctgat tgggaagtcg
     7201 cgaacgaacg gtttgcgatc gtccgcggtc cgtacgtacc aggcgtcgac ttccgattcg
     7261 tcgatgatgg agatcgccaa tccggccgcg gtgattggcg ccgcggctgg tgcggcgccg
     7321 aggcgttcga gatcatcgcg gcgtgcccgc aaggcgtcga gtgtttcctg cgccgcctcg
     7381 cgaacctggc gatgcacggc gagcgtggcg cggcggtgtg ccatatcgtg ctcgaactgc
     7441 agctcgtcgt tgtaggcggc attcttcgcc ttgtcggtcg catcgatctg cgcgacgagg
     7501 cggtcgagtg tttcgcgcgc ctcgcgcagc cgcggagcga tcgaggttgc gtacttgaac
     7561 gcggcgaacg cgccgaagag gatcaggaaa acgacggcgg tccatgtcac ctggcgcagg
     7621 ttctggcgca ggatgtcgaa cgcgtaagga atcgcgtaga tcgccaccac gccgccggct
     7681 gcccatccga gtttctgcca gagccagatg cgccgcgctt cgagtgcttc gcgcagcgcc
     7741 tgttccgcgc gcggcttctg cgcgaacagt tcgcggagtt gctgctcttt cgcctcgctt
     7801 tccgttgcca gccgcgctgc ttccgcctcg cgtgcggcgt ttcgagccgc gttgttctcc
     7861 gcctcctcgg cgaagatgac ctcgggaagc tggtgcagga gcgactcacg ttcgcgatcg
     7921 agctcctcga tctgctccgc ggcgctgtct gcagcaaccg tttcggcgac gagcttctga
     7981 tcgcgcagca tggccgacag ctcgcggacg cgcttccgcg cggtgtcgct cgcggctgtg
     8041 ttcggcgtga agcggatgcg cgcgtcgagc gctgccgtcg cagcgcggat ctcagtcact
     8101 acgttgcgcg gcgcggtatc ggcgtcgtcg cggacatccg gcaggggatc gatgagggcg
     8161 ccggtaaatg cagccgccga agcgtagtcg tcgcgatcga gcgtttcgtc gaccacgtgc
     8221 gagagcagtt cggccgcagc gctcgctgtc ttctcacgct gctcctcgag tgcgcggagg
     8281 ttcttcagat gaacggaatc acggtgttgt tgcagttcgg cgcgtgcgcc ggcgatcagc
     8341 tcatcggcgc tgcgcatctt ctcgccgacg acactcttcg gctggaagcg gcggaaaagc
     8401 gattggcccg cgtcgaggcc gatgcgcgag agcgggacgg cgaagtcttc gccgacgatg
     8461 aactgtttcg cggcgagcgc ggacgaggag tgcgcgacag gattgcccga tccgcagagc
     8521 gctacgaaga gctccgacgc gaagcgtgtc tccaggcgtt gctgcgccgc ttctcgcggg
     8581 aacacgagct ccgcgcagcc gaacgagctg aaggccggcg gcatgccgcg cgggttgatg
     8641 ccgggcagcg cgccgctcat ctccggctca aacgtgagcg cgcccgcaac ggcgtcggca
     8701 tacgtgtcaa gtgacgtgac gagtgtgccg aacttcactc gtgcgccgtt cgtagcgtcg
     8761 agcagccaaa cctcgtcaaa cggcttcgga tcggcaccac tcaatgtctt cagcacgccg
     8821 tacgcggccg cgtagccggc gtcgttcgtt gcgcccgtta cttccggcag caagcagaga
     8881 acttcgatcg agaagtagga cgccaggcgg cgcatctcgt acgttgtgcg gatggtcgcg
     8941 atcgtatcga gcagggtcgt gcgcgcggcg ccggcggcgg aaacgacgac cagcaccaac
     9001 tcgcgcgcgc tgatgtcgtc gcgcgatcgg agcgggctgt cgggccgttg ccgctggacg
     9061 tgaatcgagt tgatcgcgtc cgtcagcgcg ccggcgagcc ggaactgtac gagatcgtcc
     9121 agaccgttcg ccgccgcgac gcggcgctgc accgcctcgg cgagtgcggc ggcatcgtcg
     9181 tcgagagcaa ggatggtgac gggcttgagc atggcgccgc tttacgacag cgtctccatg
     9241 cggcgcacgt agtcttcgat cgcgatcacc tcgtgctcca cctggtcctt caccgccgca
     9301 tcggagacgc cggacatctg ctgcgacagt gaaatggtgt actccttcag aatcgaggcg
     9361 acgcgcacgg ctccttcgcg cgtgacgacc gcatcgatcg cctcctccgc ctcctgcacg
     9421 agctcgcggt tcttttcgaa cgcggcatac gccggaacgc gcccctggcc gaggcgcagg
     9481 aggttgttgt caacgcgccg gccgctcttc gatttcatcg agtaccacgc gccatcgcgc
     9541 ttgatgatgg cgaatggctc cggcgcttgc gccagcgcaa accagcgcaa cgcttcgccg
     9601 tcgccgctgc gcgggatgag gttcggaaga tcctgccagc gtttgtcgac gtgattcgag
     9661 atcactttgt tcggatcgag gtaggcgcgc tccatgtcgt cgatgtcggc gagcgcgaag
     9721 agtgggatcc ctgtcttcac tttgaacagc agtacgcgtt gcgcatcgtg cgtcgagacc
     9781 atcgaaggcc gcgcgcttcc cttcggcacg cccgtttcgt accgcgattc cttgagcatg
     9841 gttgtgtccg cgtcgccgac gccgtagtgg tagaactcgt tgatgacgga ctggttcacg
     9901 accgggatct tgccggcgtc gtaacgccac agcgggacgg cgaggttgtc gacgcgcttg
     9961 agctcctgcg acacggcctc gggagacagg cgtccgagga tgtcgtccat tgtcagcgag
    10021 gtgagcggct cgtagcactt ccgcacaaac tcgcggacat ctgcgtagac ctcgtccgcc
    10081 cgcaatgtcg accactgcgc gagcgagccg ttgtggtgat cggcatacca cttcacgaag
    10141 tcctcgccgg tcaccttcgg ccggtgctgg ccaggatcga acgagacgac ttgctcgaac
    10201 agccgtgaag cgccccgagg gttcgttgcg tcgaggtact gctgctcgaa ctgcttgcgc
    10261 gtggcgttga ggttctcgcg gatgcgataa cacgcctgca ggatcgattg cgtgtgcgcg
    10321 tggaggaccg agtagagctc cgccgccttc tccagccgcg cgatcttgat cgtcagttcg
    10381 gtctcgcggt cgacgaggcc gccatagacc tcacacgcgg tcttgatgcg gccttcgcgc
    10441 ttgaagaacg cgccgaccgc ctctccaatc tgatcctcgg ccggcttgaa gctgagcgcc
    10501 tttagctttg cgcgctcctc cttcgcctcg ccgtccatca tgttccggta ccactcgagc
    10561 ttcgcctgca gcttttcgaa gaaccgctcc gcgcgtgtga agccgttgtt gcggttcaca
    10621 gcggcgtccc accacgtgtt gatcgcggcg acggactggt cgcgcagagc ctcgaagttt
    10681 ttcgtgatcg cctgtgcgat ctgccgctcg gcgcgtgagc ggtgaacggt gtgcaggttc
    10741 ttgatctccg tgggggcctt gttgtcgaac tttagttgtt gaagcgacat cgggaatcgc
    10801 aactgtccgc cgccttcgcg ctggctgagg ttctcgatga cctggtcggc gttgtcttcg
    10861 cggagacggt ttgcgtcgat gaagcgctgc acgtcctctt cgatctccgc atcgacgacg
    10921 gtgccattga gcagctcatc gttcaccagc cgcttcgcgt cttcccacat cagcggatcg
    10981 aagctcggca tcacgagcga cgcgacgccg aagctgcagt actgcgggga acgtcctttc
    11041 acgcggccgg cggtcgcgat ctgcgtcttg atgttgtcga ccgcgttcgc gctgtcggcg
    11101 ccgatctgcg acccgatctg cacgtacatg ccgtcagcaa tgaggccgta gaggtcctgc
    11161 tgttcgctca cgacgcgtcc ctgttcgttg atgttgtcga tgaggtagac gagatcgaac
    11221 ggcggacgcg tgacgtcgac gcgccgcacg ccgtagtcga tctgaaagcc tcccttcatc
    11281 gtcgagagat actcgacctc cttgagcgcc ccgtacgcat tcggcttcac cagcgccacg
    11341 ccggcgtagc gcgtgaagat gccaggaaga aggagcacgc cggtaaagtt cgactgctcg
    11401 tcgctgccga tgatgtcgcg ggccatgaag gcgatgtcga ggaacgtgcc gctgcccgtg
    11461 ccgccggcca cgctgccgac gatttgcact tcaacgccgg cgcgcgctga gacctggaac
    11521 tggtcggcat acatctgctt ctgattgcgg atgttccgta cgtcatcgac cgcgcggcgg
    11581 atgcgaccgt agacgtcgtt cgcgcgcgcg aagagcgcga ggcggccgcg cgcccgcacc
    11641 tgcccggcgc cggcgatgat cgactgcgtc ggaatctgtg atggccacca ttcgtcgatg
    11701 tgcgcattcg tgccgttcac gagcgcggcc ggattgccga cctgcagcac gaagatctcg
    11761 ttgtgatcga gcgttccctc ggcctgcgct ttctcgatcg cggcgccgcc ttctgcattc
    11821 tcggtcgtgt cgatcgcgag aaagctgatg atcggtggga cgaagccgta ggcatcgatg
    11881 aaacgtttct tcagcttcag cagcgtgccg tagccggtgc cgccaaggcc gatgacgacg
    11941 gtgggacgaa agacgacgtt gtttttcaca gttcctcctc gacaggactg ccggtccgtt
    12001 cgtgaccgac tcggttaaaa cgcaacttcg cgtcgccgat cgcaatggtg tccgcgtcgt
    12061 aaagcgcgct ggtgggggct tcgacgtcgt tgac
//
LOCUS       DATLVW010000827         5284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_570310, whole genome shotgun sequence.
ACCESSION   DATLVW010000827 DATLVW010000000
VERSION     DATLVW010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570310"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1315
                     /locus_tag="VNN25_24165"
     CDS             155..1315
                     /locus_tag="VNN25_24165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH94693.1"
                     /translation="MPSFRDNFRALPPAAWILFAGTFINRFGTFVIPFLILYLTRIGF
                     TSAKAGLAVGAYGLGHLAASMLGGHLADRIGRRNTIVLSMFGSASAMLALSQARAYAA
                     IVILTCITGSLAELYRPASYALLGDLVPEEHRVVAFGLYRFAVNLGFAAGPATAGFLA
                     DHSFALLFVADATTSVVYGLIALFALPHGLRTWVKSERSGEALRVAMHDRPFLIFLLA
                     TLCITVVDFQMGSTFALHVKSLGFPSRAYGLLISMNGMLIVVFELGITQWTRRLPPRP
                     MIALGYLLAGVGFALTGMARTLPALAATVVIWTLGEMVSSPVASAYAVQLAPERYRGR
                     YLGLVMMMWSFGMVIGPSAGTFVFERNPAALWTACGALGVLGAGLMLRRQSN"
     gene            1611..2954
                     /locus_tag="VNN25_24170"
     CDS             1611..2954
                     /locus_tag="VNN25_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH94694.1"
                     /translation="MKNARLWLAALLLACVSANASPADVPVVYDKLPNGLKVVISEAH
                     AAPTVTVAVYYGIGFRVEPKGQTGFAHLFEHMMFQGSEHVKKFEHAKIVEANGGNLNG
                     STRLDYTNYYETLPSDRVETALWLEADRMRSLDVSDENLKNQQNVVSEEVRVNVLNQP
                     YALFEWLDLWQAANQNWFNAHNFYGDLHDIEAAKIDDVRKFFKTYYAPNNAVLTIVGD
                     VDAAAVRKMVDKYFASIPQQTIPKRPDVSEPPQTKEKRVKQTDKLANVPAFAIGWHLP
                     DQNSADYAPAVLLNLILQGDDGSRFYERLVKEKQISIDWSGGINYDLGNEFDYDGPML
                     LVSRTLYKPGHTADDILREVDAVIHDLQQKGPTARELADAKVRFRSNYYDALETTAGK
                     ANLLAAFALFRDDPSQINKALPVFDAVTAAQVQSVAQKYLVPENRTTIDRVPEAK"
     gene            3023..4489
                     /locus_tag="VNN25_24175"
     CDS             3023..4489
                     /locus_tag="VNN25_24175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH94695.1"
                     /translation="MSDADSRTRRTAKLTDNRQPTTRGPLGPRTSAIAAAALAFATIA
                     FAQATPPPPGPARPIQWPSIIESKLGNGLTVVLVPLHNVPKITAELSFLAGRGTAYKT
                     EPGVAQLAARVANEGTATRTSIEIKQQLRSIGGALTIGVDNDATTMTASSLADFAPKL
                     LDLLSDVARHPAYPQSEVALAKTNLAQEIEEQRSTPDFLAQEELDKALFGAHPYGFTV
                     PDPASIKNVSRASLKTFAATYYAPNNATLVVVGDFEPAAMHAEVERAFGPWKRVPLPP
                     APKVVLPVRDRRQIYVIDRPGSVQSTIIIGAPAPPRRSPDYIPLRTTNMIYGGSFYSR
                     LTRNIREGKGYTYSPYSTANLRRAAGSFETGASVRNEVTGPTILEMLYELDRMRVEPV
                     TAEELDSAKTYSIGGMELELEAQSSLASRITSIYIDELPHDFLQTFRDKITAITPAEV
                     QRSAARYFDTYRGAIVVVGDYKAIKDQLAPFGDVKVVK"
     gene            complement(4504..>5284)
                     /locus_tag="VNN25_24180"
     CDS             complement(4504..>5284)
                     /locus_tag="VNN25_24180"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXH94696.1"
                     /translation="KEKASPLRAVALSPALPRILSGAAGAVAMTNLGQFGIRGGANVS
                     GGFESMYAEAVAGALGGTAKESFDAARILKSTDPQKLQPENGAVYPAGPLANSLRQIA
                     QLIKSNVGLEVAFADVSGWDTHAGEGGAQGQLANNLRTFSDAIAAFTRDLGSRMGDVV
                     LVTMSEFGRTVRENGNRGTDHGHANVMLVLGGGVKGGKVYGKWPGLASQQLYENRDLA
                     ITTDFRDVFAELLTKQMAVPSLKSVFPGYDASPSKWRGILA"
BASE COUNT          954 a         1823 c         1554 g          953 t
ORIGIN      
        1 cacctgcaaa ccgcccggct tacacctgca aaccgcccgg ctcacactgc aaaccgcccg
       61 gcttacactt cacaccaccg gctcacactc tgaatgatcg ggttgctcct ccccaccccc
      121 cacgccccac cccccactcc ctatactccc cccgatgccg agcttccgcg acaacttccg
      181 cgccctgccg cccgcagcgt ggattctctt tgccggcacg ttcatcaatc ggttcggcac
      241 gttcgtcatc ccgtttctca tcctctatct cacccgcatc ggattcacga gcgcgaaggc
      301 ggggctcgcg gtcggcgcgt acggactcgg ccatctggcg gcttcgatgc tgggcggcca
      361 tctggccgac cgcatcggac ggcgcaacac gatcgtcctg tcgatgttcg gatcggcgtc
      421 ggcgatgctg gcgttgtcgc aggcgcgcgc gtacgccgcg atcgtgattc tgacgtgcat
      481 caccggctcg ctcgcggagt tgtaccggcc ggccagttac gccctcctcg gcgatctcgt
      541 gccggaggag catcgcgtcg tcgcatttgg gctctaccgg ttcgccgtca acctcggctt
      601 cgccgccgga ccggccacgg ccggattcct ggctgatcac tccttcgcgc tgctattcgt
      661 cgccgacgcc acgacgtcgg tcgtgtacgg cctcatcgcg ctattcgctc tgccacacgg
      721 attgcgaacc tgggtgaaga gcgagcgatc gggtgaggcg ctgcgcgtgg ccatgcacga
      781 ccgccccttc ctcatctttc tgctggccac gctctgcatc acggtcgtcg actttcagat
      841 gggttcaacg ttcgcgcttc atgtgaagtc gctcggattt ccgtcgcgcg cctacggcct
      901 gctgatctcg atgaacggca tgctcatcgt cgtcttcgag ctcggcatca cgcagtggac
      961 gcgccgcctc ccgccacgcc caatgatcgc gctcggctac cttctggctg gagttggctt
     1021 cgcactcacc ggcatggccc gaactctccc cgccctagcc gccaccgtcg tcatctggac
     1081 gctcggcgaa atggtcagct cgccggtggc cagcgcctac gcggttcaac tcgcacccga
     1141 gcgctatcgc ggccgctacc tcggcctggt gatgatgatg tggtccttcg ggatggtgat
     1201 cggcccgtcg gccggcacgt tcgtcttcga gcgcaacccg gccgcactct ggaccgcgtg
     1261 tggcgcgctc ggagtgctcg gcgccggtct catgctgcgg cgtcaatcca actgaaaacg
     1321 taggtcggac tctctagtcc gacatcccaa aattcttcgc acatcgcacg attcgggcaa
     1381 ggtcacgttc cagtcggact agagagtccg acctacgtcc acctcccctc ccgcgaaacg
     1441 taggtcggac tctccagtcc gacatcccaa ggtttttcgc gcatcgcacg attctggcaa
     1501 cgtcacgttc cagtcggact aaagagtccg acctacgttt cgtggcagac aaatgcgccg
     1561 caaccgtttc gttcccatct tgcaagccat ccggcgttac gattgccgtc atgaaaaacg
     1621 ctcgtctctg gctcgcggcg ttgcttctcg cgtgcgtgag cgcaaatgcc agtccggccg
     1681 atgtaccggt cgtctacgac aagctgccga acggcttgaa ggtcgtcatc tccgaggccc
     1741 acgcggcgcc gaccgtgacc gtagccgtct actacggcat cggctttcgc gtcgagccga
     1801 agggacagac cggattcgcg cacctattcg agcacatgat gtttcagggc tcggagcacg
     1861 tgaagaaatt cgagcacgcc aagatcgtcg aggccaacgg cggaaacctc aacggctcga
     1921 cgcggctcga ttacacgaac tactacgaga cgctgccgtc tgaccgggtc gaaaccgcgc
     1981 tgtggctcga agcggaccgc atgcgctcgc tcgacgtcag cgacgagaac ctgaagaacc
     2041 agcagaacgt cgtcagcgaa gaggtgcgcg tcaacgttct caaccagccc tacgcgcttt
     2101 tcgagtggct cgatctctgg caggccgcca atcagaactg gttcaacgcg cacaacttct
     2161 acggcgatct tcacgacatc gaggcggcga agatcgacga cgtacgaaag ttcttcaaaa
     2221 cctactacgc gccgaacaac gcggtcctga cgatcgtcgg cgacgtcgac gcagccgccg
     2281 tccgcaagat ggtcgacaag tatttcgcct cgatcccgca gcagaccatc ccgaagcggc
     2341 ctgatgtcag cgagccgccg caaacgaagg aaaagcgggt caagcagacc gacaagctcg
     2401 ccaatgttcc ggcgtttgcc atcggctggc atctacccga tcaaaactcg gccgactacg
     2461 cgccggcggt tctgctcaat ctgattctgc aaggcgacga cggctcccgc ttttacgaac
     2521 gactggtcaa ggaaaaacag atctccatcg attggtccgg cggcatcaac tacgacctcg
     2581 gcaacgagtt cgactacgac ggcccgatgc tgctcgtctc acgcacgctc tacaaaccgg
     2641 gccacaccgc ggacgacatc ctgcgcgagg tcgatgcggt catccacgac ctccagcaga
     2701 aaggcccaac cgcccgcgag ctcgccgacg ccaaggtccg cttccgctcg aattactacg
     2761 atgctctcga gacgaccgcc ggcaaagcca atctcctcgc cgccttcgct ctcttccgcg
     2821 acgatccttc ccagatcaac aaagccctgc cggtcttcga cgccgtgacg gccgcgcaag
     2881 tgcagtccgt cgcgcagaag tacctcgtgc cggagaaccg gacgacgatc gatcgcgtcc
     2941 cggaggcgaa atgattgcac ttcgtgcgtt agattattgc gcttcgcgcg ttgtcggttg
     3001 tcggttggtt gcgcttcgcg cgttgtcaga tgccgactcg cgaacccgcc gaaccgcgaa
     3061 actaaccgac aaccgacaac cgacaacgcg cggcccgcta gggccgcgca caagcgcgat
     3121 cgcagcggca gccctggcct tcgcgaccat cgcgttcgcg caagccacac ctccgccgcc
     3181 cggacccgcc cgcccgattc agtggccctc gatcatcgaa tcgaaactcg ggaacggact
     3241 caccgtcgtc ctcgtcccgt tgcacaacgt accgaagatc accgcggagc tctcgttcct
     3301 cgcgggccgc ggtacggcgt acaaaacgga gcctggcgtg gcgcagcttg ccgcgcgggt
     3361 ggcgaacgaa gggacggcca cacggacgag catcgagatc aagcaacagc tccgttcgat
     3421 cggcggcgcg ctgacgatcg gcgtcgacaa tgacgcgacg acgatgacgg cctcgtcgct
     3481 ggccgacttc gcgccgaagc ttctcgacct gctcagcgac gtggcgcgcc atcccgcgta
     3541 tccgcaatcc gaggtggcgc tggcgaagac gaatctggcg caggagattg aggaacagcg
     3601 atcgacgccc gacttcctgg cgcaggagga gctcgacaag gcgctattcg gcgcgcatcc
     3661 ctacggtttc acggttcccg atcccgcgtc gatcaagaac gtgtcccgcg catcgctgaa
     3721 gacgttcgcg gcgacgtact acgcgccgaa caacgccacg cttgtcgtcg tcggcgactt
     3781 cgagcctgct gcaatgcatg ccgaggtgga gcgcgcgttc ggaccgtgga agcgcgttcc
     3841 gctgccaccg gcgccgaagg tcgtgcttcc ggttcgcgat aggcggcaga tctacgtcat
     3901 cgaccggccc ggctcggtgc aatcgacgat catcatcggc gccccggcac cgccgcgccg
     3961 ctcgcccgac tacattccgc tgcgtacgac gaacatgatc tacggcggct cgttctattc
     4021 gcgcctgacg cgcaacatcc gcgagggcaa ggggtacacc tactcgccgt acagcacggc
     4081 caatctccgc cgcgccgccg gcagtttcga gaccggcgcg tcggtgcgca acgaagtgac
     4141 ggggccgacg atcctcgaga tgttgtacga gctcgatcgc atgcgcgtcg aaccggtgac
     4201 cgccgaggaa ttggattcgg ccaagaccta cagcatcggc gggatggagt tggaactgga
     4261 ggcgcagtcc agtctggcca gccgcattac gtcgatctac atcgacgagc tcccgcatga
     4321 tttcctgcag acgttccgcg acaagatcac agcgatcact cccgccgagg tgcagcgcag
     4381 cgcagcgcgc tacttcgaca cctaccgcgg cgccatcgtc gtcgtcggcg actacaaggc
     4441 gatcaaggat cagctagcgc cgttcgggga tgtgaaggtg gtgaagtagg tctgctttca
     4501 tcactacgcc agaatccctc tccacttcga cggcgatgcg tcgtatcccg ggaacacgct
     4561 cttcagcgac ggcacagcca tctgcttcgt cagcagttcg gcgaagacat cgcggaagtc
     4621 agtggtgatg gcgaggtcgc ggttctcgta gagctgctgc gatgcgaggc cgggccactt
     4681 gccgtagacc ttgccgccct tgacgccgcc gccgaggacg agcatgacgt tggcgtggcc
     4741 gtggtcggtg ccgcggttgc cgttctcgcg gactgtgcgg ccgaactccg acatggtcac
     4801 gaggacgacg tcgcccatgc gcgatccgag gtcgcgcgtg aaggcggcga tggcatcgga
     4861 gaaggtgcgc aagttgttcg cgagctgtcc ctgcgcgcca ccttcgccgg cgtgcgtgtc
     4921 ccatccgctc acgtcggcaa acgccacttc caggccgaca ttggatttga tcaactgggc
     4981 gatctgtcgc aacgagttgg caagcgggcc agccggatag acggcgccgt tctctggctg
     5041 cagtttctgc ggatcggtgc tcttgaggat gcgcgcggcg tcaaacgatt ccttcgccgt
     5101 tcccccaagc gctcccgcca ccgcctcggc atacatggat tcgaatccgc cgctgacgtt
     5161 cgcaccgccg cggattccga actgcccgag gttcgtcatc gccaccgcgc cggccgcgcc
     5221 ggagaggatg cgcggcaacg caggtgagag cgcgaccgcg cgcagcggcg aagccttctc
     5281 cttc
//
LOCUS       DATLVW010000828         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_445350, whole genome shotgun sequence.
ACCESSION   DATLVW010000828 DATLVW010000000
VERSION     DATLVW010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445350"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VNN25_24185"
     CDS             complement(<1..460)
                     /locus_tag="VNN25_24185"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HXH94697.1"
                     /translation="MSPESARLCCSCSMARADDHLPDDTDALKAALTETRAKLVGAQA
                     LIEHLQLVIAKMQREKFGPRSEKSQRLIEQMELQLEELAAASAEDEAKTEAAAGVEVR
                     GFSRRKATRRNFPADLPRRRVVHPAPTTCPCCGGSNLSKIGEDITETLDVV"
     gene            1018..3105
                     /locus_tag="VNN25_24190"
     CDS             1018..3105
                     /locus_tag="VNN25_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXH94698.1"
                     /translation="MKSELVKSAHLARKAVVYIRQSTPHQIVSNQESLRLQYALRQRA
                     RELGWHEADIDVIDADLGLSGASAAQRSGFKELVGRVGLSEVGLILSIDVTRLARNCS
                     DWYPLLDICGLRGCLIADRDGVYDPGSPNGRLLLGLKGTISELELHTIRSRLTSGLLA
                     KAERGELALGLPIGLVRDPSGIVIKDPDLAVQERIGLVFQTFLKVRTVAKVMRVLNDR
                     GLDLPRRDRHGEPCWARATVASVATILKNPAYAGAFVYGRTRMRKIMREGASSAKVPR
                     PMEEWRFVVKDRYPAYIDWPTYEKIRSIIRDNRAEYMRTKTRGVPRDGELLLHGIAWC
                     ARCGYKMYVRYKGGGEYVCNHLRCHEGLPACQHIRAPQIDAAVADAFLTALAPAELDA
                     LSRARKAQHQMDAALRTSAERQLERKRYAAALAERQFNRVDPDNRLVADELERRWEAA
                     LKELRAAEEALAKQAAPEAITRIAMGKALNNKVIALAGRLPQIWQNENTTDTQRKALL
                     RCLVEKVVLDRGEYDVATVRIVWRGGAVTDLEVKMNVNSVVKLTRGTEMRDRVLDLAR
                     AGMLDDEIADVLTGEGHRSPNCAEKVLPITVQRIRLSAGIKIAAQRNRWSHDPRVLSA
                     PELAAKLNIPVNWLYVQIRQKRLLIDRQPTGAYLFRNTPSVMDAVRSLRSHSINQLDL
                     RISQPHKEGHQHA"
     gene            complement(3093..>3393)
                     /gene="tnpB"
                     /locus_tag="VNN25_24195"
     CDS             complement(3093..>3393)
                     /gene="tnpB"
                     /locus_tag="VNN25_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HXH94699.1"
                     /translation="PVPSGVRVWLAVGRTDMRKGMNGLALQVQQTVGRDPHAGDLYVF
                     RGVRGNLIKIIWHDGIGMSLYAKRLERGRFIWPSADSGVVSISTSQLAYMLDHAC"
BASE COUNT          707 a         1027 c         1027 g          632 t
ORIGIN      
        1 gcaccacatc gagcgtctcg gtgatatcct caccgatctt tgagagattg ctgccaccac
       61 agcacgggca cgtggtcggc gccggatgaa cgacgcgccg gcgcggcaga tcggcaggaa
      121 agttgcgccg cgtcgccttg cgccggctga agccccgcac ctcgacacct gctgctgctt
      181 cggtcttggc ctcatcctca gccgatgccg ccgccagctc ttcgagctgc aattccatct
      241 gctcgatgag ccgctggctc ttctcagagc gcggaccgaa cttctcacgc tgcatcttgg
      301 cgattaccaa ttgcaaatgc tcgatcagcg cttgcgcgcc cacgagcttg gcgcgcgtct
      361 cggtcagcgc agccttgagc gcatcggtat cgtcgggaag gtgatcatca gcccgagcca
      421 tgctgcaact gcagcacaag cgcgctgatt cgggcgacaa atcgcgcccc ctgagtcaac
      481 gagtcgcggc cttgttcaac cctcagactt cgatgaactg taagcggcaa aaaaacggcc
      541 ggttacgatg aactcaggct tcgacaagct cagccggccg cggtgggacg ccaagtgtgc
      601 tggggattgc gccaatcaat cccgtcgagc gaaggcttac aggaatatcc cttctggtga
      661 gtctgacgaa tcatcgatcg aagtgatcgg ggtccgcgac ccgcgacatc ctctgtatgg
      721 acgatcgttc cgtgtgatcc gtcgcgtgat ggatcgtggc ggtaatgttc cagcatctta
      781 tgaagtcgaa tatcgcaatg gatcgagtct aattatacct gtccaagcga cagaagagtg
      841 tgagttaaat tcgaatcaga ctaaactaag catcgaggcg ttattggata ttgtatctgt
      901 ggctgaatct atcgacgacg atgaatatag atccaaaata cctttggtca gtgctgtcgc
      961 cgacgctgcg gcgtcaaatc gtcgacgacg tcgctgccgt cctggtggag gtcgctcgtg
     1021 aagtccgaac tggtgaagtc ggcccatctg gcgcgcaagg cggtcgtcta tatcaggcag
     1081 tcgacaccgc accagatcgt gagcaatcag gagagcctgc ggctgcaata cgcgctgcgc
     1141 cagcgcgccc gcgaactcgg atggcatgag gctgacatcg atgtaatcga tgccgatctt
     1201 ggcctgagcg gcgcgtccgc cgcgcagcga agcggcttca aggagcttgt cggccgggtc
     1261 ggactcagcg aggtcgggct gatcctctcc atcgacgtga cgcgtctcgc ccggaattgc
     1321 tccgattggt atcccctcct ggatatatgc gggttacgcg gctgcctcat tgccgatcgg
     1381 gacggcgtgt atgatcccgg cagtcccaac ggccgcctgc ttctcggtct gaaggggacg
     1441 atatccgagc tggagcttca tacgatccgc agccgtctca cgagcggcct cttggcaaag
     1501 gccgaacgcg gcgagctggc gctcgggctg ccgattgggc tcgtgcgtga tccgagcgga
     1561 atagtcatca aagatcctga cttggctgtg caggagcgga tcgggcttgt attccagacc
     1621 tttctaaagg tccgcacggt agccaaggtc atgcgtgtgt tgaatgatcg tggcctcgac
     1681 ctgccgcgcc gcgaccgaca cggcgagcca tgctgggcgc gagcgacggt cgcgtcagtc
     1741 gcgacgatcc tgaagaatcc tgcgtatgcc ggcgcattcg tctatggccg aaccaggatg
     1801 cgcaaaatta tgcgtgaggg cgcgtcgtcg gcaaaagtcc cgcgaccgat ggaggaatgg
     1861 cggttcgtcg tgaaggatcg atacccggct tacatcgatt ggccgaccta tgaaaagatc
     1921 cggagtatta tcagagacaa cagagccgaa tacatgcgca cgaagacgag aggagtcccg
     1981 cgagacggcg agcttctgtt gcacggcatc gcctggtgcg cccgatgcgg ttacaagatg
     2041 tacgttcgct acaagggcgg cggagagtat gtctgcaatc atctgcggtg tcatgaggga
     2101 ttgccagcct gccagcatat ccgcgccccg cagatagacg cggccgtggc ggatgcgttc
     2161 ctaacagcgc tggcgcctgc cgagctcgac gctctgtcgc gtgcccgtaa ggctcagcac
     2221 cagatggacg cggcactgcg cacaagcgcg gaacgtcaac tcgaacggaa gcggtatgcc
     2281 gccgcgctgg cggagcgcca gttcaaccgt gtcgatccgg acaaccggct tgtcgccgac
     2341 gaactcgagc gtcggtggga agcggcactg aaagagctgc gggcggccga agaagcgctc
     2401 gccaaacagg ccgctcccga ggccatcact cgaatcgcaa tgggcaaggc tcttaacaac
     2461 aaggtcatcg cgctggccgg acgcctcccg cagatatggc agaacgaaaa caccactgat
     2521 actcaacgca aggcgctgtt gcggtgcctt gtcgagaaag tcgtccttga ccgcggcgaa
     2581 tatgacgtgg ctacggtcag aattgtgtgg cgtggcggtg ctgtgaccga tctcgaagtg
     2641 aagatgaatg tcaactccgt cgtcaaactc acgcggggca cggaaatgcg cgaccgcgtg
     2701 cttgatctcg cgcgcgccgg tatgctcgac gatgagatcg ccgacgtcct caccggcgag
     2761 gggcatcgtt cacccaactg tgcggaaaag gttctgccga tcacggtgca gcggatccgg
     2821 ctcagcgccg gaatcaagat tgccgctcag cgtaatcgat ggtcccatga cccccgcgta
     2881 ctcagcgccc cagaactggc cgccaagctg aacatcccgg tcaactggct ctacgtgcag
     2941 atcaggcaga agcgcctcct gatcgaccgt cagcccaccg gagcctatct cttccgcaac
     3001 acaccctccg tcatggacgc agtccggagc ttacgaagcc acagcatcaa ccagcttgac
     3061 ctgagaatca gtcagcctca caaggagggg catcaacatg catgatcgag catgtaggcg
     3121 agttgcgatg tcgagatcga gaccacgccg gaatcggccg atggccaaat gaaccggccg
     3181 cgctccagcc gcttggcata gagcgacatg ccgatgccgt cgtgccagat gatcttgatc
     3241 aaattgccgc ggacaccccg gaacacatag agatccccgg catgcggatc gcggccgacc
     3301 gtctgctgta cctgcagcgc caaaccgttc atgcctttcc tcatgtcggt ccggccgacc
     3361 gccagccaca cccgaacacc gctcggaacc ggg
//
LOCUS       DATLVW010000829         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_33746, whole genome shotgun sequence.
ACCESSION   DATLVW010000829 DATLVW010000000
VERSION     DATLVW010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33746"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="VNN25_24200"
     CDS             complement(<1..2085)
                     /locus_tag="VNN25_24200"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH94700.1"
                     /translation="MRRLRVIELMAVVVALALLPEPTATWQREPGKRPTARRRIIARV
                     VGEAYAPRLVFRGFDPGTNDPPAPPLTDDPHDRGRVLLRRGEVSKAIPALREAARNGD
                     PAALTDFAAALLGEAELLPWRHPRERASSRLDPSLRYASALEAIAVLRRAIEQQPGLA
                     AAHFNLALALEQIGLTVEVQTQLEEAIRLSDDVEWDEDARQHEESLPLSDPWIDRDRI
                     RAYVEVTSLAAARRLLQPYLAAQEQRLRRARPPVYPDDRFFLAADTYTRDVRSIGGHL
                     TQAMQTFCPRAKAEPCAMRYYTAGALYAAGRKSEAAAWLYSIEDEIQRAHGAAGLRAL
                     RQWEEGLNLYVRGRSMSALEVFDAQYAEHRASGERVLAEVFDELRRAARPYPMSQALS
                     GGDIETAFRYADDSSLRDVQNALAPDAAILRYATTFEDHKVVFVIRRDSVDVVTLYDT
                     INRRPGDQVGFVAHGDSVDVISMDGHPDSTRRGGAEITGTAARMRTAPDTVTASLLHE
                     LVVAPVLEKLHGISTIAVIPNVELAEIPFGALFDAGRGQYLAERFTIVHAPSARAAVE
                     LSKRTRHSHDSTLLAIGATEFDRTQGEILAGVDREIAEITAQALCPRVLSGEQATPDA
                     VQRALAENAVIHYGGHIVRRGADPRLLLASARGRDSLSAEEIAALRLDKARVVVLAGC
                     RGAASGAPHAFVR"
     gene            2604..>3094
                     /locus_tag="VNN25_24205"
     CDS             2604..>3094
                     /locus_tag="VNN25_24205"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXH94701.1"
                     /translation="MIADSATAVSVRDLVLAHVALFRLYSALPLVLLSCGIGAALGVA
                     PLHLAAAATAMICGLAGGYAYNDLRDQQLDRLNRPRRPLVSGVLPEPYVRRMVLMLYG
                     SALLFAAATASLRTIAFVVLLIGSACLYSDAIKHAPGLKNVFVGVWCGLLPWGTALDR
                     TTAA"
BASE COUNT          530 a          993 c          995 g          576 t
ORIGIN      
        1 tcgaacgaat gcgtgaggcg ctccacttgc agcgccgcgg catccggcca aaaccacgac
       61 gcgcgctttg tcgaggcgga gagccgcaat ctcctccgca gagagactgt cgcgaccgcg
      121 cgcggaagcc agcaacagcc gtggatccgc gccgcgacgc acgatatgtc cgccgtaatg
      181 aatgaccgcg ttctccgcga gcgcacgctg aactgcatcc ggcgtggcct gttcgccaga
      241 gagcacccgt gggcacaggg cctgcgctgt gatctctgca atttcacggt caactcccgc
      301 gagtatttct ccctgcgtac ggtcaaactc cgtcgcgccg atcgcgagca gcgtggaatc
      361 gtgggaatga cgcgtgcgct tcgacagctc caccgcggca cgcgccgatg gcgcatgcac
      421 gatcgtgaag cgttcagcca gatactgacc acgcccggcg tcgaacagcg caccgaaggg
      481 aatctccgcg agctcaacgt tcggtatgac ggcgatcgtg gagatgccat ggagtttttc
      541 cagcactggc gcgacgacga gttcgtgcag cagcgaggct gtcaccgtat ccggcgcggt
      601 gcgcatcctc gccgctgtcc ctgttatttc cgctccgcct cgacgcgtgc tgtcagggtg
      661 cccatccata ctgatcacgt ctacggaatc gccgtgagcg acaaagccaa cctggtcccc
      721 tgggcgtcga ttgatggtgt catacagagt caccacgtct acggagtctc tgcgaatgac
      781 aaagacgacc ttgtggtcct cgaacgtggt tgcgtaccga aggatggcag cgtccggggc
      841 aagagcattc tgtacgtcgc gcagcgacga atcatcggcg tatcgaaatg ctgtctcgat
      901 atcaccgccg gacaatgctt gactcattgg atatgggcgg gcggcccgtc gaagctcgtc
      961 gaacacctct gcgagcacgc gttcgccgga agcccggtgc tccgcgtatt gagcatcaaa
     1021 aacctcgagt gcgctcatcg aacgaccccg aacgtacagg ttgagcccct cttcccattg
     1081 ccgcagcgcg cgcaaccccg cggcaccgtg agctcgctgg atctcgtctt cgatcgagta
     1141 aagccatgcg gccgcttcag atttccggcc ggcagcgtag agcgcaccag ccgtgtaata
     1201 gcgcatcgcg cacggttcgg ccttcgcgcg ggggcaaaac gtctgcatcg cctgggtgag
     1261 atgtccgccg atcgaacgga catcgcgggt ataggtgtcc gcggcgagga agaacctgtc
     1321 gtccgggtaa acgggaggcc gcgcgcgtcg gagtcgctgt tcctgcgccg ctagatacgg
     1381 ctgcaacaga cgcctcgcgg cagcaagact ggtgacctcg acgtatgccc gaatgcggtc
     1441 ccgatcgatc cacgggtcgg aaagcgggag actctcctca tgctgtcggg catcctcgtc
     1501 ccattcgacg tcgtcagaca ggcggatcgc ctcctcgagt tgcgtttgga cctcgaccgt
     1561 aagcccgatt tgttcaagcg cgagagcgag gttgaagtgc gctgctgcga ggccaggttg
     1621 ctgctcgatc gcgcgtcgca acaccgcgat ggcttccagc gccgacgcgt agcgtaacga
     1681 gggatccagc cgcgacgatg cgcgttcccg tggatgacgc caggggagaa gttccgcttc
     1741 accgagtagt gccgccgcga agtccgtcag cgcggcggga tcgccgttgc gcgccgcctc
     1801 gcgcaatgcg ggaatcgcct ttgagacctc gccacgtctc aggagtacgc ggccgcgatc
     1861 gtgaggatcg tctgtcagtg gcggggccgg agggtcattg gtgccgggat caaagccccg
     1921 gaagaccagt cgcggggcgt atgcctcacc cacgactctc gcgatgatgc gccgacgcgc
     1981 ggtgggacgt ttccccggct cgcgttgcca cgttgctgtc ggctcgggca gtagtgcgag
     2041 tgcgacgacg accgccatca gttcaattac gcggagcctg cgcataccgt aagtttagaa
     2101 gggacaagcc tccgcttttt tccgccgcgt cgtcgcgcac gcccgtgctc cttccgcgac
     2161 gctgctagcg aacgaggatc gcagccggcg acggcgagta gcggtgccca ggcgatatgc
     2221 gtgtgcatcg tggcacaacg caaatcgtta tgcagtttgt ttagccctcc ggcctgtcac
     2281 gccggaggtc gcgggttgga ggcccgtcgg ccctgccaaa ctaaagctct catgcgctgc
     2341 gacttcgcgg cgtttcgtgt cttctgaggt tgctttggtc actcgcaggg tcgcgattct
     2401 cgcgggcgga attctgcgca gacgcaagac caaaacagcg aagcgttttt ggtttcggcg
     2461 caagtgttcc acggaaaatg catcaggtgg ccagatggat cgccccgccg gaccgctgca
     2521 tcaacaacct gccggtcggt tgcgagtacg tccgggtggc atgacggtac tatactgcgc
     2581 attcccatgt tgcggaatgt cccatgatcg ccgacagcgc gaccgctgta agcgtgcgcg
     2641 atctcgtgct ggcgcacgtc gcgttgttcc ggctctactc ggcgctcccg ctggtgctgc
     2701 tctcctgcgg catcggagct gcgctcggcg tggcaccgct gcacctggcc gccgcagcga
     2761 cggcgatgat ctgcggactc gccggcggct acgcgtacaa cgacctgcgc gaccagcagc
     2821 tcgatcgctt gaacaggccg cgccggcccc tggtgtccgg cgtgctgccg gagccgtacg
     2881 tgcggcgtat ggtgctcatg ctgtacggca gcgcgctgct gttcgccgcg gccactgcgt
     2941 cgctgcgcac gatcgcattc gtggtgctgc tcatcggcag cgcctgcctc tattcggatg
     3001 cgatcaagca cgcgccggga ctgaaaaacg tcttcgtcgg cgtctggtgt ggcctcctgc
     3061 cgtgggggac ggcgctcgac cggacgacgg ccgc
//
LOCUS       DATLVW010000830        21690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_204588, whole genome shotgun sequence.
ACCESSION   DATLVW010000830 DATLVW010000000
VERSION     DATLVW010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21690
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204588"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VNN25_24210"
     CDS             <1..651
                     /locus_tag="VNN25_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXH94702.1"
                     /translation="NVVGTLRLLEAAADAGVKRIVFASTGGAIYGEPVEVPQGETHPT
                     EPLSPYGCAKLAVEHYLHYFRVVQGLSSVALRYANVYGPRQNAHGEAGVVAIFAKRML
                     NGQTVTINGSGEQTRDFVYVGDVVAANLAASEAEWQGEYNVGTGVETSINTLFQTLAS
                     IAGLAAPSEFAPAKAGEQMRSVLDGRRLRTLAALPEPVMLREGLNTTFGWFRKSAR"
     gene            complement(696..2294)
                     /locus_tag="VNN25_24215"
     CDS             complement(696..2294)
                     /locus_tag="VNN25_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94703.1"
                     /translation="MPTTHSVHGERELPLDPEPPISFSHLAHTLRAYAVVIVLSMAGV
                     AIAYSIVAIALYLFAPTLKTTSLSFRLDFEGAERRQFPNGLRFSPSEIVSAPVLVKVF
                     DNNHLERFTNFGNFSKSIVILESNPAYELLSAQFQSRLADPKLSPVDRERIENEFEMK
                     RASLAKNEYSINYLRGFDRGVPETMIRKVLTDTLAVWAQFAINEQHALDYRVSVLSPQ
                     ILDVPSVEPDDYVTRIQVLRSKVFRIIDNVGELQKMPGADLARTRDHLSLNEIQFRLE
                     EIVRFRFEPLVGLVRNSGLIANPALTTRFLENQLAYDQRLLQARQSEAEIIRQALAVY
                     TEQRGESIASSGEGPNGKTGAARVTGETVMPQLSDTFIDRLLTLTTQSADTQYRQHLV
                     NDYRLATAKTIPLQQAVAYDTQILNEVRNSAVAAQRSDPSVVQRQIEQSVDEVRQLIL
                     KMNEIYQVVSRNTNPSTQIYAITDTPVSHTETAFSASRLLLYGALVLLIAFPITVVAC
                     LLHNRVREEEAEEAYEEEAVQAGS"
     gene            2445..4304
                     /gene="asnB"
                     /locus_tag="VNN25_24220"
     CDS             2445..4304
                     /gene="asnB"
                     /locus_tag="VNN25_24220"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH94704.1"
                     /translation="MCGIAGFVSFDGERSAESLRTTAEGMAARLVHRGPDDEGTWVDP
                     PAGVALGFRRLRIIDLTKAGAQPMTSASGRYVIVFNGEVYNFERLREELQRADAAPAW
                     RGHSDTEVMLACFETWGVQKAVGRFIGMFAFAVWDRHERALILGRDRAGVKPLYVAGE
                     GKNLLFASQIDALAAHPDFMMAIDRGAAGLYARLGYVPAPFSIARGVQKIRPGALLVV
                     KEDGTRTEEQYWSPANVAASTAVFQGSEDEALVALDVLFQDAVSLRMIADVPVGIFLS
                     GGVDSALVAAMAQRGTSTPVKTFTIGFDDRQLDESDAAARVAAHLATCHEELRVDERA
                     LLDAVPLMPQIYDEPLADTSAIPTYLVARLARPHVTVALSGDGGDELFGGYHRYFLGR
                     KMWRPIERVPRPLRKPLGLVLGAAGFASERIRRYGAAFRARDAIAFHAAALLDERLPI
                     SGDPALPFVLNQRSEWPRLRDVTELQMYLDFASYLPDDILMKVDRATMAVSLESREPL
                     LDHRLINFAWSLPVSMKVRDHTGKWILRRLLQRYVPESLIGAEKRGFGLPIAHWLRTA
                     LRDWVEANLPRDDDYFDRLAVQSLWKRHLAGQNHDALIWRVVMFEAWRRARAL"
     gene            4344..4901
                     /locus_tag="VNN25_24225"
     CDS             4344..4901
                     /locus_tag="VNN25_24225"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXH94705.1"
                     /translation="MPNLRRESEIFPADLFSMPDGGPWTIVHVRSRQEKAVARLFMER
                     KQPFYLPQIEKQVRRDGRTFKSFLPLFGGYVFVRLNDDTQQTLWRTKAVVRMIPVDDQ
                     ELLDGELRQIRALQEAGAILVAHPEIAAGDVVRIADGVFQGYTGTVVRELDATRLVVS
                     ITALNKSVTAEFPRESVVPARAAKR"
     gene            5235..6593
                     /locus_tag="VNN25_24230"
     CDS             5235..6593
                     /locus_tag="VNN25_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXH94706.1"
                     /translation="MTTETAPLVESPASLHALIEKIDARAARVGVIGLGYVGLPLVLL
                     FAESGFEVTGFDVDPKKAQALNAGESYIRHIDASRIRAAFVDHTRASASIDFEQLASC
                     DAIIICVPTPLGDHREPDLSYVRATADAIAKTLRRGQLMILESTTYPGTTREELLPRF
                     EAGGLTCGRDFFLAFSPEREDPGNESYSAKTIPKVVGGVDGPSLKAAAALYGAVVDHV
                     VPVASAEVAESAKLLENIFRSVNIALVNELKVVFDRMGIDIWEVIDAAKTKPFGFMPF
                     EPGPGLGGHCIPLDPFYLTWKAAEHGVWARFIELAGEVNTSMPRYVVEKTAAALNDAG
                     KSMKGARVLVLGLSYKAGIDDDRESPSFEILALLRARGAETAYCDPNIPVARPGRKHD
                     LHLASVACTRRSFAEYDAIVISTAHAEFKEPDLYRDARIVVDTRNIVSAASVMSGRLY
                     KA"
     gene            6590..7597
                     /locus_tag="VNN25_24235"
     CDS             6590..7597
                     /locus_tag="VNN25_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HXH94707.1"
                     /translation="MILVTGAAGFIGYHVSRSLLDRGEDVLGIDNLNDYYDPALKEAR
                     LDRLRPYPKFRFRRDDIADRAAIFEVFADEKPRGVIHLAAQAGVRHSLTNPHSYVTSN
                     LEGFLNILEGCRHNAVEHLVYSSSSSVYGANVTQPFSEHDNVDHPVSLYAATKKANEL
                     MAHSYSHLYGMPTTGLRFFTVYGPWGRPDMALFKFTRGILAGEPIPVFNNGEMIRDFT
                     YIDDIVEGVVRVLDHAPVANPSWPREHPDPATSYAPYRVFNIGNNRPVPLLDCIAALE
                     KAVGKTATLNLLPMQPGDVPSTMADVSDLENAVGFRPNTPIETGIARFVEWYRKFYST
                     S"
     gene            7602..7982
                     /locus_tag="VNN25_24240"
     CDS             7602..7982
                     /locus_tag="VNN25_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HXH94708.1"
                     /translation="MNPTVEDEMRYRLLRYFEQHPHASQRELARELGISVGKVNYCLR
                     ALIQRGWVKMRNFRDSTNKLAYTYTLTSKGIEERVSVASRFLRRKLAEYDALSQEIER
                     LTRDLREDRDQAQPESTPSPAPSR"
     gene            8007..9101
                     /gene="gmd"
                     /locus_tag="VNN25_24245"
     CDS             8007..9101
                     /gene="gmd"
                     /locus_tag="VNN25_24245"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXH94709.1"
                     /translation="MTVVKATKRALVTGITGQDGSYLAELLLQKGYEVHGIVRRSSSF
                     NTSRLDSIYQDPHEQNRRLILHYGDLNDASSLNRILRVVDPDEIYNLGAQSHVRVSFD
                     TPEYTGEVTGLGCTRLLEAIRELEIRPRIYQASSSEMFGRVLETPQSEKTPFYPRSPY
                     GVAKVYAFWITVNYREAYDLFAVNGILFNHESPRRGETFVSRKITRAVARIAAGMQDR
                     LYLGNLEARRDWGYAKDYVEAMWRMLQHDVPNDYVVATGDTRTVREFCERAFAEVGID
                     LAWRGEGLEEKGVDRESGKVIVEIDSRYLRPTEVDLLVGDASKARQDLGWAPTTGFEE
                     LVRLMVQSDVKLAESEARAAGAEGVRSMVE"
     gene            9176..10756
                     /locus_tag="VNN25_24250"
     CDS             9176..10756
                     /locus_tag="VNN25_24250"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXH94710.1"
                     /translation="MIPPSQDSLAADLEPSTSPYVTVRSWLARGRTGRAFAGLVVDFL
                     GSSSSIVIALLFTPMVVAWLGAPMYGFWIAASQIILWLSLLDGGIGANLMKTISTHRA
                     SAPERVERAIASTFWCYVCIAVATLLIGASMTPFVSRWMHLHGEAARQGTITFAVAVF
                     TSVVALVAVPTFYVILNGFQKLALVNGLVSGVSIAGTIGGLLFVWAGLGVIGLAVAQL
                     LATLAGAVLALLWSRRLFPFRLSLRYVSRSELVEMLRFALYFTMSKTAFLTSNYSDGI
                     LIAMFYGTAPVAVFALTQKLSSAAFMFVVKVGSVAVPGLAELMGAGDRPALRSVITRM
                     TRILVRIAILAATIILTLNHRFVAAWTGEATFGGALLTALFAWGIFRDTIIRNLSAPL
                     FASGDLKGWGLLSSAEAVLKVGLSIALLPILGIVGPMVGTAVAELITGVYTPFKLIRL
                     LDIDPRTIFSDAILPPFLRSLPTVAVMTFLAFVVPAHWRWFGIVTIASAGVLANIIAF
                     DLSHIATFFRQPQFGRTA"
     gene            10786..11733
                     /locus_tag="VNN25_24255"
     CDS             10786..11733
                     /locus_tag="VNN25_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011223720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HXH94711.1"
                     /translation="MGEHRDFWSGKRVLVTGGAGFLGSHIIERLERRGATTFVPRAGN
                     YDLTREADVKRCYDDADTDIVLHLAAKVGGIGANRPHPGSFFHDNALMGIQLIEEARI
                     RGVAKFVCLGTVCAYPKFAPVPFREDDLWNGYPEETNAPYGVAKKALLVMLQGYRQEY
                     GLNGIYLLPVNLYGPRDNFDLVTSHVIPAMIRKFLEAKEAQAQSVSLWGDGTPTREFL
                     YVEDAAEGILAAAEKYDGADPVNLGSGEEISIRELAETIKALTGFEGQIVWDPTQPNG
                     QPRRKLDTSRALERFGWRSTTSLRDGLKRTLDCYLNLQQ"
     gene            11990..12196
                     /locus_tag="VNN25_24260"
     CDS             11990..12196
                     /locus_tag="VNN25_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94712.1"
                     /translation="MFDFASLRTELEAVCFSDIHKYDWRPTEHADVDDDSQAYIPHME
                     KQTGVLISLNVECSKPVQSVSAAV"
     gene            12242..13228
                     /locus_tag="VNN25_24265"
     CDS             12242..13228
                     /locus_tag="VNN25_24265"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXH94713.1"
                     /translation="MSSSYWEGLRVLVTGGAGFIGSYLTELLLDDGAVVTVADNLSSG
                     RMANLADVISRIRFEECDLRYLDACERIAAGQDVVLNLAAPAFGVEYSMTHHGEMLTD
                     TVLIGFNMLEAARRQGVTRFLVVSSSCVYPDDAALPTNESEGWRGSPETVNEGYGWGK
                     RMVELQGQYYAREYGMEVAIARPFNAYGPREQIEKEKAHVMTALIQKVLGNDNPVIVW
                     GSGNQTRSFVHARDFALGLKLIAEHHAVADPVNLGHDRETSIRDLAERIVQLSGSGSS
                     IRYDLSKPEGAVRKSADVRKLQRVTRFTPQIMLDDGLKEMIGYVRERASPGE"
     gene            13221..14369
                     /locus_tag="VNN25_24270"
     CDS             13221..14369
                     /locus_tag="VNN25_24270"
                     /inference="COORDINATES: protein motif:HMM:NF013048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HXH94714.1"
                     /translation="MSDSAVSTRTRWNSPRKFILFHAARATEADFFGEQSRDNCAQPF
                     IALRKRCEELGYTLDVTKSHPLEECEWIIFWDVDSLGPMGAIEWLLQRAKRLAYHRPF
                     RNLYREALSSAHRPGMALIVAEPPSISRRNLKTDVHGAMDVVLTWNETWLAGGGKYVR
                     TILPITSDFPSPAVVDFDEKKLLVDISANKFSGHPAELYGQRREAIRYFERRFPRDFD
                     LYGVGWNKGRTWTLGRRLRTGDKHHYSSYRGPVAHKWDVMPRYRFAICYENAIFEDYV
                     SQRIFDVLRCNCVPVYWGAPNIDKYVDPGAFVDRRHFSSNAELGDYLAGVGSIEYQSM
                     IDAGRRYLETDRFRQFLGEAWGENLLSALSLGGKKSASGDVDCGHVQL"
     gene            14341..15309
                     /locus_tag="VNN25_24275"
     CDS             14341..15309
                     /locus_tag="VNN25_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HXH94715.1"
                     /translation="MLIVVTFSSDSRIYVAGHAGLVGSALVTRLRKASCENLILRTRQ
                     ELDLTSRDAVFQMFEQTRPEFVFIAAARVGGIAANMASPVEFLVENLDIQNNLLLACA
                     RFRVAKTLFLGSSCIYPRDASQPMREDDFMTGPLEPTNESYAIAKIAGIRLARALRAE
                     HGVNVICAMPSNVYGPGDHFDLTRAHVASALVRRFGEAKQNLAPSVGVWGTGSARREL
                     LHVADLADACLFLMEHYDSPELVNVGTGEDVTIRELAETIGRLLEYQGEIQWDATKPD
                     GMPRKVLDVSRLHGLGWRHSIGFEDGLQSLIGAFNSWQAGSKAEPA"
     gene            15306..16580
                     /locus_tag="VNN25_24280"
     CDS             15306..16580
                     /locus_tag="VNN25_24280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXH94716.1"
                     /translation="MKNLGYTESLREPLQALMNEASRINSYKPQPYWYPLSIATYDVE
                     EVLAAVDSLCSFRTTMWEKTVRFEDEFARLHGVNEAIMVNSGSSADLLIAFAMTNPAA
                     RMKLQPGDEVLLPSVTWPTQIWSVMMAGLKVRLVDTDPKTLNVDLDDLEAKIGERTRA
                     ISLVHLMGNPCDMDRVTALCQKHGLLLVEDCCEALGARFGGKPVGTFGLAGSYSFFFS
                     HHITTMEGGAVICSDPELSDLLRLLRAHGWARNTRHMTPVVADGLDPRYMFLNWGFNV
                     RPTELQAGFGLEQLKRLGAFQSQRLENAKYLLRNLERYASLIQSIEVHPKGECSWFAV
                     PLILHESCPFDRSALLRHLEANGVETRPIVAGNVARQPVCAMFPDLQGPPLPGADLVH
                     ERGLYIGLHPFAAKESLDRLTEVFDSFLKVYI"
     gene            16649..17398
                     /locus_tag="VNN25_24285"
     CDS             16649..17398
                     /locus_tag="VNN25_24285"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXH94717.1"
                     /translation="MPRESRYLYRLSKKYVDRYRNENNGDMRSNGEERFLASVLPACR
                     VVFDVGANVGDWAKIALSVNPRVNLHCFEPSHATYARLVASRFPPNVLCNNIGLGSTP
                     SEGRLLIFEDGSGLNSLYRREGLEAGWGIDTNTREESIQLDTLDNYVESQGITSRIDL
                     CKIDVEGHELEVLRGMKATLGRRQMGMIQFEYGGCNIDSRVLLKDIFSFLHGFKYELF
                     KIWPAELRHVPKYDQRLENFQYQNWVAKPTI"
     gene            17403..18473
                     /locus_tag="VNN25_24290"
     CDS             17403..18473
                     /locus_tag="VNN25_24290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94718.1"
                     /translation="MGIGPPMTERVLFVASLYNDGILAHGLSYEYYNLYKPLEQAVGN
                     VDTFDFMQRMHEIGGERMNGLLLETVKRDRPALVLVVPFRDEFDPAVMDDLMKCTTTV
                     AYFFDDIWRREYSEFWAPHFTYVTTSDVNGVRRFREAGSNNAVYSPFACNHEIFVKKD
                     LEKIYDVAFVGQFHPNRAWWIRELRRAGINIAVWGHGWDTGSVGEEDLVRIFNQARIS
                     LNLSNSNSLDLRYVLALRRPISETLRTWKRTARAFMRGDSKNREQVKGRHFEISACGG
                     FQISYYVEGLERHYQIGEEIALYASPDDLIETIRYYLKHDDEREAIALRAYERTWREH
                     TMARRFRDLLGQTGHAELLVAP"
     gene            18496..19380
                     /locus_tag="VNN25_24295"
     CDS             18496..19380
                     /locus_tag="VNN25_24295"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH94719.1"
                     /translation="MAARRRPMLKERILTNTDKTAIRTNACEYSRVYEGAAPRISLWL
                     HIRAKVHSLKRWFSPIDDFNWDDYPAHYRAENEFNRRFFTDDLRDVEFLFTNGKLYLL
                     ADCKPLNQTHRCVYEAICNLPGIRSIAEIGVGGGRYLANLRLLMGKDVALSGYDRAPL
                     QIKFFEELFPEVFRETTTGVMDLTEASIKEESVPDLVYAVTVLMHIQRPDAYRNALSN
                     LLASGRHFVLLLDHWNAHDYFTDLTAYAASDPSARLYTYDSGANIAVVVARNGRTLDT
                     PYVPLTDPAALQRYLRPL"
     gene            19768..20730
                     /locus_tag="VNN25_24300"
     CDS             19768..20730
                     /locus_tag="VNN25_24300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94720.1"
                     /translation="MSPDSFSAKVLESSSPMFSVVIPTFNREKKVAKAVRSVLAQTFT
                     DFEIVVVDDGSDETARQLQDEFGDRVRYFRGLGQRVAGARNLAIGHALGEWIAFLDSD
                     DWWYPTKLERYAEAARAHPEIGLFYSMMDMVDDRGRYIRTLPIRTRRDVYPAVLEGNF
                     IFNSTVAVKRECLSRVGLFDTKLIGCEDWELFVRVTREFPALLIDESLVAYEHSSSGS
                     LSSNHEVWVAAHDEVIAKCLAADTTLSSAWVQRIHAGGHYVQARIYLGAGDECHALEN
                     FRAAFRQNRRRWRAFVYMILLSWKPSRMILSRRAKVWLRLPEAR"
     gene            21400..>21690
                     /locus_tag="VNN25_24305"
     CDS             21400..>21690
                     /locus_tag="VNN25_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94721.1"
                     /translation="MESARGSIEATEALVKLDEGSSWSIRLGDILWEYGRVGEACAAY
                     RRAIALPASASFAAERARGRLCESNEDWQGALKHYERSIRLLIDKNTLSAFDA"
BASE COUNT         4278 a         6151 c         6606 g         4655 t
ORIGIN      
        1 aacgtcgtcg gaaccttgcg tctgctcgaa gccgctgcgg atgcaggagt gaaacgaatc
       61 gtctttgctt cgactggtgg cgcgatctac ggcgagccgg tcgaagtgcc acaaggagag
      121 acgcatccaa ccgagcctct ctcgccatac ggctgtgcga agctcgcggt cgagcactac
      181 cttcactatt tccgcgtcgt gcaaggcctg tcgtccgtcg ctctgcgcta tgccaatgtc
      241 tacggtccgc ggcagaacgc acacggagag gcaggcgtcg ttgccatctt cgcgaagcgc
      301 atgctcaacg ggcagacggt gacgatcaat ggcagcggcg agcagacacg cgacttcgtc
      361 tacgtcggcg acgtcgttgc ggcgaacctc gcggcttccg aggccgaatg gcagggggag
      421 tacaacgtcg gcaccggagt cgagacctcg atcaatacgt tgttccagac gctggcttcg
      481 atcgccgggc tggcggctcc atccgaattc gcaccggcga aagcgggcga acagatgagg
      541 agcgtgctcg atggccggcg gctacggaca ctggccgcgc tgcctgaacc ggtgatgctg
      601 cgcgaagggt tgaacacgac tttcggctgg tttcgaaaga gcgcgcggta ggcgcgttcc
      661 tgttcacaga gccagctgct gattgttcag aaggttcagc ttcccgcctg aaccgcttct
      721 tcctcgtatg cctcttccgc ttcttcctcc cgcacgcggt tgtgcagaag gcaggcgacg
      781 accgtgatgg gaaacgcgat gagcagcacg agggcgccgt acagcaagag gcgcgacgca
      841 ctgaatgccg tctccgtgtg ggagaccggc gtatccgtaa tggcgtagat ctgcgtcgat
      901 ggattcgtat tgcgcgagac cacttgatag atctcgttca tcttgaggat gagctgacgc
      961 acttcgtcca cggactgctc gatctgccgc tgaacgaccg acgggtcgct ccgctgcgcc
     1021 gccactgccg agttgcgcac ctcattcaga atctgggtgt cgtaggcgac tgcttgctgc
     1081 aggggaatcg tcttcgccgt cgccaggcgg tagtcattca cgagatgttg ccggtactgt
     1141 gtgtcggccg actgtgtggt tagtgtcagg aggcggtcga tgaaggtatc gctgagttgc
     1201 ggcataacgg tctcgccggt tacgcgggca gctccggttt tcccattcgg gccctcgcca
     1261 gatgacgcga tcgattcgcc acgctgctcg gtgtagacgg ccagcgcctg acggatgatc
     1321 tcggcttcgc tctgccgcgc ctgcagaagt cgctggtcgt acgccagctg attctcgagg
     1381 aagcgagtcg tgagcgccgg atttgcgatg aggccgctgt ttcgtaccag tccaacgaga
     1441 ggctcgaagc ggaatcgaac gatctcctcg aggcggaact ggatctcgtt gagggagaga
     1501 tggtcgcgcg tccgcgccag gtcggcgccc ggcattttct gcagctcgcc gacattgtcg
     1561 atgatgcgaa acaccttcga gcgcaacacc tgaatcctgg tgacgtagtc gtccggctca
     1621 acggatggaa cgtccaggat ttgcggcgac agcaccgaaa cgcggtagtc gagcgcgtgt
     1681 tgttcgttga tcgcaaactg cgcccagacc gcaagcgtgt ctgtcagaac tttgcggatc
     1741 atcgtctcgg gcactccgcg atcgaaaccc cgcaggtagt tgatcgaata ttcgttcttg
     1801 gccagactgg cgcgcttcat ctcgaactcg ttctcgatgc gctcgcggtc gaccgggctc
     1861 agcttgggat cggcgaggcg cgactggaat tgcgccgaaa gcagttcgta ggccgggttc
     1921 gattccagga tcacaatcga cttgctgaaa ttgccgaaat tcgtgaaccg ctcgaggtga
     1981 ttgttgtcga agacttttac gagaacgggt gcacttacga tctccgaagg agagaaccgg
     2041 agcccgttcg ggaattgcct gcgctccgct ccctcgaaat cgagacgaaa cgacaggctg
     2101 gtggttttga gcgtcggagc gaaaagatag agggcgatcg ccacgatcga atacgcaatg
     2161 gcgacgccgg ccatcgacag aacaatcacc acggcgtagg cgcgcagcgt atgggcaagg
     2221 tggctgaatg agatcggggg ttcgggatcg agaggcagct ctcgctcccc atgcacactg
     2281 tgagttgttg gcactgaaat tggctcctga ctgctgagat agaatgtcag cgcagcaggc
     2341 gctcgtcaag catggctgtc tgcatcgctc acgaatggat tcgctctcac acctcctttt
     2401 cgcttcgcaa ttcgccgtcc gctgctgcat ttagttagcc tccaatgtgt ggaatcgctg
     2461 gttttgtctc cttcgacggt gagagaagcg cggaaagcct tcgcaccacc gccgagggaa
     2521 tggccgcgcg gctcgtgcac cgcggtccgg atgacgaggg gacgtgggtt gatccccctg
     2581 ccggagttgc gctcggattt cgccgtctgc gcatcatcga cttgaccaaa gccggcgcgc
     2641 agccgatgac ctctgcgagc ggccgctacg tgatcgtctt caacggtgag gtctacaact
     2701 tcgagcgttt gcgtgaagaa ttgcagcgcg cggacgccgc gccggcctgg cgcgggcact
     2761 ccgatactga ggtcatgctg gcgtgcttcg agacgtgggg tgtccaaaag gccgttggtc
     2821 gcttcatcgg catgttcgcc tttgccgtct gggaccgcca cgagcgtgca ttgatcctcg
     2881 ggcgcgaccg cgccggcgtc aagccgctgt acgtggcggg tgaggggaag aatctcttgt
     2941 ttgcgtcgca gatcgatgcc ctcgccgcgc atcccgattt catgatggcg atcgaccggg
     3001 gagctgcggg actctacgcg cggctcgggt atgtgcctgc tccgttttcg attgcgcgcg
     3061 gcgtgcagaa gatccggccg ggagcgctgc tggtcgtgaa agaagacgga actagaacgg
     3121 aagagcagta ttggtcgccg gcgaatgtcg ccgcgtcaac agccgtgttt caggggagtg
     3181 aggacgaggc actggtagca ctcgacgttc tcttccaaga cgccgtttct cttcgaatga
     3241 tcgccgacgt tcctgttggt attttcctca gcggcggcgt cgactccgcg ctggtcgcgg
     3301 ccatggccca gcgaggcact tcgacgccgg tgaagacctt cacgatcgga ttcgacgacc
     3361 ggcagctcga tgagtccgac gctgctgcgc gcgtcgcggc acatcttgcc acctgccacg
     3421 aggagttgcg cgtcgatgag cgtgcgcttc tcgacgcggt accgctgatg ccccagatct
     3481 acgacgagcc gctggccgac acatccgcaa ttccgacgta tctcgtcgcc cggctggctc
     3541 gcccgcacgt gaccgtcgcg ctctcaggcg atggcggcga tgagttgttc ggcggctatc
     3601 atcgttactt cctggggcgg aagatgtggc ggccgatcga acgtgttcct cgacctctgc
     3661 gcaaaccact ggggttggtc cttggcgcgg ccggtttcgc cagcgagcgc attcgtcgtt
     3721 acggtgcggc cttccgtgcc cgtgacgcga tcgcatttca tgctgctgca ctgcttgatg
     3781 aaaggctgcc gatcagcggc gatcccgcgc tgcccttcgt gttgaatcag cgctccgaat
     3841 ggccacgcct gcgtgacgtc accgagctcc agatgtatct cgacttcgcc tcataccttc
     3901 cggacgacat tctgatgaag gtcgaccgcg ccacgatggc cgtgagtctg gagtcacgcg
     3961 agccgcttct cgatcatcgg ctgatcaact tcgcctggtc tctgccggtg tcgatgaagg
     4021 tcagggacca taccgggaag tggattcttc gccgtctgct acaacggtac gtgcccgaat
     4081 cgctgatcgg cgccgaaaag cgcggcttcg gcctgccgat cgctcactgg ctccgcacgg
     4141 cactccgcga ttgggtcgaa gcgaacctgc cccgcgacga cgactatttc gatcgcctcg
     4201 cggtgcagag cctttggaaa cgtcatctgg caggtcagaa tcatgacgcg ttgatctggc
     4261 gcgtcgtcat gttcgaagcc tggcggcgag ctcgcgcttt gtgagggaat ttcgcgcacg
     4321 cgcctggccg tcgttaggcg cgcatgccca acttgcggag ggaaagcgag atctttccgg
     4381 ccgatctctt ttcgatgccg gacggcggac cgtggacgat cgtccatgtt cgcagccgtc
     4441 aggagaaggc ggtggcgcgc ctcttcatgg aacggaagca acccttttac ttgccccaga
     4501 tcgagaagca ggttcgccgc gatggacgga cgttcaagtc gttcctcccg ctttttggcg
     4561 gctacgtttt cgttcggctc aacgacgata cgcagcagac gttgtggcgg acgaaggcgg
     4621 tcgtccggat gattccggtc gatgatcagg agttgctgga tggggagttg cggcagattc
     4681 gtgcgttgca ggaagcaggc gcgatcctcg tcgcgcatcc ggagatcgcg gctggagacg
     4741 tcgtacgaat cgccgacggc gttttccagg gttacaccgg tactgtcgtc agggagcttg
     4801 acgcgacgcg gctagtcgtc tcgattacgg cgctgaacaa gtcggtcacg gcggagtttc
     4861 cgcgtgagag tgtcgtcccg gctcgcgccg cgaaacggta atgggacgtg acagttgcgc
     4921 gttgccggtc gatatggttg aattggctgc gttcaactat tgaacgccgc ccggtttggc
     4981 cggtccctcg atcagcatcg tgaggggttt acccggtaac cgaagcatgc gcgggggaca
     5041 gaagaaccct gacgcgcctc atacgtgagt ttgttggaaa tcgtgtcggc gcggaccaat
     5101 tccgcgtctg caagcccggg agggtgagcg ctctcccatc agaagttcgc gatgtcgatg
     5161 ccggtcgaag gcgccgtcta ctgtggcggc atgtacaccg aaaggggcga agcctgcctc
     5221 gtttctgaat cggaatgacg accgaaaccg cgcccttggt cgagagtccg gcatcgctgc
     5281 atgcgttgat cgaaaaaata gacgcgcgcg ccgcccgcgt cggtgtcatc gggctcgggt
     5341 acgtcggcct gccgctggtg cttctctttg cagagtcggg tttcgaagtc accggtttcg
     5401 acgtcgatcc gaagaaggcc caggctctta atgctggtga gtcgtacatt cgtcacatcg
     5461 atgctagccg gatccgcgct gcgtttgtcg atcacacgcg agcctcggcg tcgatcgact
     5521 tcgagcagct cgcctcgtgc gatgcgatca tcatctgcgt tccgaccccg cttggcgatc
     5581 atcgcgagcc cgatctctcg tatgtccgcg ccacggccga tgccattgcg aagacacttc
     5641 ggcgcggcca actcatgatt ctcgaatcga cgacctatcc cggcacgacg agggaagagt
     5701 tgctgccacg tttcgaggct ggcggtctca cgtgcggacg cgatttcttc ctcgcctttt
     5761 caccggagcg tgaagacccg ggcaacgaga gctactccgc caaaacgatc ccgaaggtcg
     5821 tggggggtgt cgatggtccg tctttgaaag cggccgcggc gctctacggt gcggttgtcg
     5881 atcacgtcgt gccggttgcg tctgccgagg tggccgagtc tgcaaaactg ctcgagaaca
     5941 tcttccgctc cgtcaatatc gcgctcgtca acgagctgaa agttgtcttc gacaggatgg
     6001 gcatcgacat ctgggaagtc atcgacgccg caaagacgaa gcccttcggg ttcatgccgt
     6061 tcgagcccgg accgggcctt ggggggcact gtatcccgct cgatccgttc tatctcacct
     6121 ggaaggcggc ggagcacggc gtatgggccc gcttcatcga gctggccggc gaagtcaata
     6181 cttctatgcc gcgctatgtt gtcgagaaga ctgccgcggc tctcaacgac gctggaaaga
     6241 gcatgaaagg tgcgcgagtg ctcgtgctgg gcctttcgta caaggccggc atcgacgatg
     6301 accgcgagtc tcccagtttc gagattctgg cgctgcttcg cgcgcgcggc gccgaaactg
     6361 cgtactgcga tccgaacatt ccggtcgcac gacccgggcg caaacacgat ttgcatctgg
     6421 cgtcggttgc ctgcacgcgc cggtcgttcg cagaatacga cgccatcgtg atctcgacgg
     6481 cacacgccga gttcaaagag ccggatcttt atcgcgacgc aagaatcgtc gtcgacacgc
     6541 gcaacatcgt ttccgcggcc tctgtgatga gcggacggct ctacaaagca tgattctggt
     6601 taccggtgcc gccggcttca tcggctacca cgtctcccga agtcttctcg atcgcggcga
     6661 ggacgttctt ggaatcgaca atctcaacga ctactacgat ccggcactga aggaagcgag
     6721 gctcgaccgc ctccggccgt atccgaagtt tcgttttcgg cgggacgaca ttgcggaccg
     6781 cgcagctatc ttcgaggtat ttgcagacga gaaaccccgt ggcgtcattc acctcgccgc
     6841 gcaggccggc gttcggcact cgctgacgaa tccgcactct tatgtcacca gcaacctgga
     6901 gggattcctc aatatcctcg aaggttgccg ccacaatgcc gtcgagcatc tcgtctacag
     6961 ctcgtcgagc tcggtctacg gcgcgaacgt cacccaacct ttttccgagc acgacaacgt
     7021 ggaccatccc gtctcactct atgccgctac gaaaaaggcc aacgagctga tggcgcactc
     7081 gtattcgcat ctgtacggga tgcccacgac cggcctgcgc ttctttacgg tttacggacc
     7141 gtggggccgg ccggacatgg cccttttcaa gttcactcgc ggcattttgg ccggcgagcc
     7201 catccccgtt tttaacaatg gcgagatgat tcgcgacttc acgtacatcg acgacatcgt
     7261 cgaaggtgtg gtccgcgtac tcgaccacgc gcctgttgcc aatccatcct ggccgcggga
     7321 acatcccgat cccgccacca gctacgcgcc ttatcgcgtc ttcaacatcg gcaacaaccg
     7381 cccggtcccg cttctcgact gcatcgccgc actggaaaaa gcggtcggga aaaccgccac
     7441 gctgaacctg ctgccaatgc aaccaggcga tgtgccctcg acgatggctg acgtcagcga
     7501 tcttgagaac gcggtcgggt tccgccccaa cacgccgatt gagaccggca tcgctcgatt
     7561 tgtggagtgg taccgcaagt tctacagcac gagttgacga tatgaatccc actgtcgaag
     7621 acgaaatgcg gtatcgcctg ctgcgatatt ttgagcagca tccgcacgcc tcccagcgcg
     7681 agctggcccg ggagctcggc atcagcgtcg gcaaggtaaa ctattgtctg cgcgcgttga
     7741 tccagcgagg ctgggtgaaa atgcgcaact tccgcgacag cacgaacaaa cttgcgtaca
     7801 cctacacact caccagtaaa gggattgaag agagagtgag cgtcgcttcg cgctttctgc
     7861 ggcgaaaact cgcagagtac gatgccctgt cgcaggagat cgagaggctg acccgggatc
     7921 tacgcgagga tcgcgatcag gcccagcccg aatcgactcc gagtccggcg ccttcgcggt
     7981 agagattttc taaaaggacc agcgtcatga ccgtagtcaa agcaacgaag cgcgctcttg
     8041 tcaccggcat taccggtcag gacggctcgt acctggcaga gttgttactg cagaagggtt
     8101 acgaggttca tggcatcgtc cggcgctctt cctccttcaa cacatcgcgt ctcgattcga
     8161 tctatcagga tccgcacgag cagaaccgcc gcctgatcct ccattacgga gaccttaacg
     8221 atgcatcctc gctgaaccgc attctacgcg tcgtcgatcc cgacgaaatc tacaacctcg
     8281 gcgcgcagag tcacgtccgc gtctcgttcg acacccccga gtacacaggt gaggtcactg
     8341 gactcgggtg tactcgcctg ctcgaagcaa tccgcgagct cgagatccgc ccgcgcatct
     8401 atcaagcctc atcttctgaa atgtttggca gagtgcttga aacgccgcaa agcgagaaga
     8461 cgcctttcta tccgcgcagc ccttatggcg tggccaaggt ctatgccttc tggattacag
     8521 tgaattatcg agaggcctac gatctcttcg ctgtcaacgg catcctcttc aatcacgaat
     8581 cgccgcgacg gggtgagacg ttcgtgtcgc gaaagattac gcgggctgtg gcgcggatcg
     8641 ccgccgggat gcaggatcgt ctgtatcttg ggaatctcga agcccggcgt gattggggat
     8701 acgcaaagga ctacgtggag gcgatgtggc gcatgctgca gcacgacgtt cccaacgatt
     8761 atgtagtggc tacaggagac acgcgaaccg tccgtgagtt ctgcgagcgc gcgttcgccg
     8821 aagtgggcat cgatctcgcc tggcgcggag agggcctcga ggaaaaaggc gttgaccgcg
     8881 agagcggcaa ggtgatcgta gagatcgatt cccgctacct gcgcccgacg gaagtcgatc
     8941 ttctcgtagg agacgcctcg aaagcgcgac aggatctagg gtgggccccc acgaccggtt
     9001 tcgaggaatt ggtgaggctg atggtgcagt ccgacgtcaa gctggccgaa agcgaagccc
     9061 gcgcggccgg tgccgagggc gtgcggtcaa tggtggaatg agaatgagcc gagataactc
     9121 gcgcgtccga tgaagtcgag aaggcgaagt cgctgatcag caaacgtctg gaatcatgat
     9181 ccctccatct caggattcac ttgccgccga cctggaaccg tcgaccagtc cgtacgttac
     9241 ggtgcggtcg tggctggctc ggggacgcac gggccgtgcc ttcgcgggcc tcgtcgtgga
     9301 tttcttgggc agctcgtcca gcatagtgat tgcgcttttg tttacgccaa tggtcgtcgc
     9361 gtggctcggg gcgccgatgt acggcttctg gatcgcggcc tcgcagatca tcctgtggct
     9421 cagccttctc gacggcggca tcggtgcgaa tctgatgaag accatcagta cccacagagc
     9481 aagtgcgccg gagcgggtcg agcgcgccat tgccagtacg ttctggtgtt acgtctgcat
     9541 cgctgtggcc actctgctca tcggcgcatc gatgacgcct ttcgtttcac gctggatgca
     9601 ccttcatgga gaggctgcgc gccaaggcac gatcacgttt gcggttgctg tgttcacaag
     9661 tgttgttgcg ctcgtcgctg tcccaacttt ctatgtgatt ctcaatggtt ttcagaagct
     9721 cgctcttgtt aatgggctgg tgagcggagt cagcatcgcc ggaacgattg gcgggctcct
     9781 tttcgtttgg gccggattgg gtgtgatcgg tctggcagtg gcgcaactgc tcgccacgct
     9841 ggccggagcg gtcctggcat tactctggtc gcggcgcctc tttccctttc gtctatccct
     9901 ccgctatgtc agccggtctg agctcgtgga aatgttgcgc tttgccctgt atttcacgat
     9961 gtctaaaaca gcattcctca cgtccaacta cagcgacggg atactgatcg caatgtttta
    10021 cggtacggcg cccgtggccg tgttcgcgtt aacccagaag ctttcttcgg ccgccttcat
    10081 gtttgtcgtc aaagtcgggt cggtcgcggt ccccgggctt gccgagctca tgggcgcggg
    10141 agatcgtcct gcgctgcgtt cggtcattac ccgaatgacc aggattctgg ttcgcatcgc
    10201 tatcctggcg gccacgatca ttctgacgct gaatcacagg ttcgttgcgg cgtggacggg
    10261 cgaagcaacc ttcggcggtg ctctcctgac cgccctcttt gcctggggca ttttccgcga
    10321 cacaatcatt cgcaatctgt cggcgcctct ctttgcgtca ggtgatttga agggatgggg
    10381 tctcttgtcg agcgcggagg cagtcctgaa ggttggcctg tcgatagcgc tgttgccaat
    10441 tcttggtatc gtgggcccga tggtcggtac cgctgtcgcg gagctgatca ccggggtcta
    10501 tactccgttc aagttgattc gtcttctgga tatcgacccc cggaccatat tcagcgacgc
    10561 aatcctgccg ccgttcttgc gctcgctgcc gacggttgcc gtgatgacct tcctcgcttt
    10621 cgtcgtgccc gcgcattggc gctggttcgg aatcgtgacc atcgccagcg ctggcgtgct
    10681 tgcgaacatc attgcattcg acttgagcca catcgcgacc ttcttccgcc agcctcaatt
    10741 cgggagaact gcttgaccat cgacagcatt gcccgggttg ccggcatggg cgagcaccgc
    10801 gatttctggt ccggcaagcg cgtcctggtt actggtggcg cggggtttct tggctcgcac
    10861 atcatcgaaa ggttggaacg ccgcggagcg acgacttttg taccgcgagc tggcaattat
    10921 gatttgacgc gcgaagctga tgtcaaaaga tgctatgacg acgccgacac cgacattgtt
    10981 cttcatctcg ccgcgaaggt cggcggaatc ggcgccaacc gccctcatcc cggaagcttt
    11041 tttcacgaca acgcactgat ggggatccag ctcatcgaag aggcacgaat ccgcggagtg
    11101 gccaagttcg tctgcctggg caccgtgtgt gcgtatccga agttcgcacc cgtcccgttt
    11161 cgagaggatg atctctggaa cggttatccg gaggaaacca acgcccctta tggggtggcc
    11221 aagaaggccc ttctggtcat gcttcagggc taccggcagg agtacgggct caatggtatc
    11281 taccttctgc ccgttaacct ctatggacca cgcgacaact tcgacctcgt aaccagtcat
    11341 gtgattccag ccatgattcg gaagtttctc gaagccaaag aagcgcaggc gcaaagcgtt
    11401 tcactgtggg gagacggaac tcccacgcgc gaattcctct atgttgagga cgccgcggag
    11461 gggatcctcg cggcggctga gaaatatgac ggcgccgatc cggtcaacct cggctccggc
    11521 gaggagatct cgatcaggga gctcgctgag acaatcaaag cgcttaccgg tttcgaaggc
    11581 caaatcgtgt gggacccgac acaaccgaat ggccagccaa ggagaaagct ggatacctcg
    11641 cgtgcattag aacgtttcgg ctggaggagc acgacctctt tacgcgacgg gttgaaaagg
    11701 actctcgatt gctatctcaa tcttcagcag tgaccgataa cctgctgttt gttcctgacg
    11761 ccagtatcga gctgatctat agctgtcacg tgctcgagca tttcaagcgg cgtgacgtcg
    11821 gccgcgtcct gcgtgagtgg caccgtgttc ttaagccggc tggagtgctc cgcacatctg
    11881 ttcccgattt tgcggcgttc tgcgagatgt accagcggaa ccaggacatc gggctggtga
    11941 tcggacctat cttcggacgc aggattatct ctacaacatt cactacaacg tgttcgactt
    12001 tgcttcactg cggacggagc tcgaagcggt ctgttttagc gatatccaca agtacgactg
    12061 gcggccgacg gagcatgcag atgtggacga cgactcgcaa gcgtatatcc cgcacatgga
    12121 aaagcagacg ggcgtactga tcagtctcaa tgtcgagtgt agcaagcctg ttcaatcggt
    12181 cagcgctgcg gtgtgatgtg atccagatcc agtctctctt tctgaattcg gaggttcacg
    12241 gttgtcttcc agctattggg aaggtcttag agtactagtt accggcggcg caggtttcat
    12301 aggctcatat ctcacagagt tgctccttga cgatggagca gtagttacgg tcgccgacaa
    12361 tctctccagc ggccggatgg cgaatctggc ggacgtcatc agtcgcattc ggtttgaaga
    12421 atgcgatctg cgttacctcg acgcctgcga aaggattgcc gcgggacagg acgtagtgct
    12481 caaccttgcc gcgcccgcgt ttggggtcga atacagcatg actcatcacg gcgaaatgct
    12541 gaccgacact gtgttaatag gcttcaacat gcttgaggct gcccgacggc agggagtgac
    12601 acgttttctt gtagtcagca gttcctgtgt ctatccggac gatgccgcgc tccctacaaa
    12661 cgagagcgag ggttggcgag gcagcccgga aaccgtcaac gaaggctatg gctggggcaa
    12721 acgtatggtg gagctgcaag gccagtatta cgcccgggag tacggaatgg aggtggcgat
    12781 tgcccggcca ttcaacgcgt atggacctcg cgagcagatc gagaaggaga aagctcacgt
    12841 catgacggca ctgattcaga aagtgctcgg aaacgacaat cccgtcatcg tctggggcag
    12901 cggaaatcag acgcgaagct ttgttcacgc ccgcgacttt gcgctcggcc tcaagctgat
    12961 agctgaacat cacgccgttg cagatcccgt aaacctcggt catgatcgcg aaacgtcaat
    13021 tcgcgatttg gccgagcgga ttgtgcaatt aagtggtagc ggtagcagta ttcggtatga
    13081 cctcagcaag ccagagggag cggtccggaa gtctgcagat gtccgaaaac tgcaacgggt
    13141 aacaaggttc acaccacaaa tcatgctgga tgatgggctc aaagaaatga tcggttacgt
    13201 tcgcgagcgg gcgagccccg gtgagtgact cggcagtctc cacgcgaacg aggtggaact
    13261 cgccaaggaa attcattctt tttcatgccg cacgcgcgac cgaggccgat ttctttggcg
    13321 agcagtcgcg ggacaactgt gcccagccat tcattgcgct cagaaagcgt tgcgaagaat
    13381 tggggtacac actcgacgtc acaaagagcc acccgctgga ggaatgtgaa tggatcatct
    13441 tctgggacgt cgacagcctc ggcccaatgg gcgcgatcga atggctgctt caacgcgcaa
    13501 agaggttggc ttatcaccgg ccatttcgaa atctgtaccg ggaagcgctc tcgagtgcac
    13561 atcggcccgg tatggcgctc atcgtggccg agcctccttc gatctccagg aggaacttga
    13621 agaccgacgt tcatggcgca atggacgttg ttctgacctg gaatgagaca tggctcgcgg
    13681 gcggtggaaa gtatgtcagg acgatccttc cgattacatc cgacttcccc agccctgccg
    13741 tcgtggactt cgacgaaaag aagctgctcg ttgatatttc agccaacaag ttttcaggtc
    13801 atcccgcaga gctttacgga caacgtcgcg aagcgatacg ctatttcgag cggcgatttc
    13861 cgcgcgactt cgatctctat ggtgtgggct ggaacaaagg cagaacctgg accctcggtc
    13921 gacgcctacg aacgggcgac aagcatcact acagttcgta tcgcggaccg gtcgcgcaca
    13981 aatgggatgt catgcctcgg tatcgatttg caatctgcta tgaaaatgct attttcgagg
    14041 actacgtgtc gcagcgcata tttgatgtgc tgcggtgtaa ctgtgttccg gtctactggg
    14101 gagctccgaa tattgacaaa tatgtcgacc ccggcgcgtt tgtcgatagg cggcactttt
    14161 catcgaacgc ggagctgggc gactatctgg ctggggtcgg atcgatcgaa taccagtcga
    14221 tgatagacgc agggcggcgc tatttggaga cggaccggtt ccgccaattc ctgggtgaag
    14281 cctggggaga gaatcttcta tcagcactat cgctaggcgg caagaagtct gcctctggag
    14341 atgttgattg tggtcacgtt cagctctgat tccagaatct acgtcgccgg tcatgccggg
    14401 ctcgtcggct cagcgctcgt cacacggtta cggaaggcgt cctgtgagaa tctgattctt
    14461 cggaccaggc aggagctcga tctcacgtcg cgggacgccg tctttcagat gtttgagcag
    14521 acacgtccgg agtttgtatt cattgccgcg gcgcgcgtcg gcggcatcgc cgcgaacatg
    14581 gcctctccgg tcgagttttt ggttgagaat cttgatattc agaacaattt gctgcttgcg
    14641 tgtgctcggt ttcgcgtggc caagacgctg ttcctgggaa gttcctgcat ctaccctcgg
    14701 gacgcgtccc agccaatgag ggaagacgac ttcatgaccg ggccgttgga gccgacgaat
    14761 gaatcgtacg ccatcgcgaa gatcgccggg atccgccttg cacgtgcgtt gcgcgcggag
    14821 cacggagtca atgtcatctg cgcgatgccg agcaacgttt acgggcccgg cgaccacttc
    14881 gatctgactc gtgcgcacgt ggcgtccgcg cttgtgcggc ggttcggaga ggcgaagcag
    14941 aatctggcac cgtctgtggg cgtatgggga acgggcagcg cgcgccggga gttattgcat
    15001 gttgccgatc tggcggacgc ctgtttgttt ctgatggagc attacgactc gccagagctg
    15061 gtcaatgtgg ggaccggcga ggatgtaacg atccgcgagc ttgcagagac gattgggcgg
    15121 ttgctggagt atcagggcga aatccagtgg gacgcgacaa agccggacgg gatgccgagg
    15181 aaggttctcg acgtatcgcg gctgcatgga ctcggctggc ggcattcgat tggtttcgaa
    15241 gatgggttgc aaagcctgat cggcgcgttc aactcgtggc aggctggatc gaaggcggaa
    15301 cctgcatgaa gaatctggga tacacagaat cgctgcgcga gccgcttcag gccttaatga
    15361 acgaggcgtc acgcatcaat agttacaagc cacagccgta ctggtatccg ctcagcatcg
    15421 ctacctacga tgtcgaagag gttctggccg cagtcgattc gctttgctcc ttcaggacaa
    15481 caatgtggga aaagaccgtg cggttcgagg atgaattcgc gcggcttcat ggtgtgaacg
    15541 aggccatcat ggtcaacagt ggctcatccg ccgacctcct gatcgctttc gcgatgacca
    15601 accccgccgc tcgaatgaag ctgcagcctg gcgacgaggt gcttctgcct tccgtgacct
    15661 ggccgacgca gatctggtcc gtgatgatgg cgggcctgaa agtgcggctg gtcgatacgg
    15721 accccaaaac gctgaacgtc gacctggacg atctcgaggc gaagattggg gagcggaccc
    15781 gcgcaatttc gctggtacac ctgatgggca atccatgcga catggatcgg gtaacagcgc
    15841 tttgccagaa acatgggttg cttttggtcg aagactgctg tgaagcgctg ggcgcacgat
    15901 tcggcggaaa gcctgtaggc acgttcgggc tggccggaag ctactcattc ttcttctcgc
    15961 accacatcac cacaatggaa gggggcgccg tcatttgctc cgatcccgaa ttgtcggacc
    16021 tcctccgcct gctgcgagcg catggctggg ctagaaatac aagacatatg actccggtcg
    16081 tcgctgacgg gctcgacccg cgatacatgt tcctcaactg gggattcaat gtgcgtccaa
    16141 ccgagttgca ggccggattc gggctggagc agttgaagcg cctcggcgcg ttccaaagcc
    16201 agcgccttga aaatgccaag tatcttctgc ggaatctgga acggtacgcc tcgctaatac
    16261 agtcgattga agttcatcca aaaggagagt gttcctggtt cgctgtcccg ttgatccttc
    16321 atgagtcatg ccctttcgac aggtccgcac ttctgcggca tctcgaagcc aatggcgtag
    16381 agacacggcc catcgttgcg ggcaatgtgg cgcgtcagcc ggtgtgcgcc atgtttcctg
    16441 atttgcaagg cccgccgcta ccgggcgccg atctggttca cgagcgaggc ctctatatcg
    16501 ggttgcatcc atttgccgcg aaggagagcc tcgatcggtt gaccgaggtc tttgactcgt
    16561 ttctgaaggt gtacatctga tcccgcctag ccagtcattc catttgtcta atcccagact
    16621 catgttcaaa gaatggttgt tttcgtgctt gccacgcgaa agtcgatatc tctaccgcct
    16681 ttccaagaaa tacgtcgacc gctatcgaaa cgagaacaac ggcgacatgc gcagcaacgg
    16741 cgaggagcgg tttctcgcgt cggtgttgcc ggcctgccga gttgtgttcg acgtcggtgc
    16801 gaatgttggg gactgggcga agatagccct cagcgtcaac ccgagggtga atctgcactg
    16861 cttcgaaccg agtcacgcta catacgcgcg actggtagcc agtcgctttc cgcccaatgt
    16921 tctctgcaat aacatcggcc tcgggtccac tccgtcggaa gggcgccttc tgatcttcga
    16981 agatggctcc ggactgaatt cgctctatcg cagggagggt ctggaagcgg gatggggaat
    17041 cgacacgaac acccgcgagg aaagcattca gctggacact ctcgacaact acgtcgaaag
    17101 ccagggcatt acgtcgcgaa tcgatctctg taaaatcgac gtggaggggc acgagctgga
    17161 agtgcttcgg ggcatgaagg cgacgctcgg ccgtcgccag atgggaatga tccagtttga
    17221 gtacggaggc tgcaatatcg actcccgcgt gcttctgaaa gacattttct cgtttctcca
    17281 tggtttcaaa tatgagctat tcaagatttg gccggcggag ctgaggcacg ttccgaaata
    17341 cgatcaacgg ctcgaaaact tccagtacca aaactgggtg gcaaagccga cgatatgaag
    17401 atgtggggat cgggccgccg atgacggaac gggtactgtt tgtggcgtca ctttacaacg
    17461 atggcattct ggcgcacggg ctgtcgtatg agtattacaa cctttacaag ccgctggagc
    17521 aggctgtagg caatgtcgat acgtttgatt tcatgcagag aatgcatgaa atcggtggtg
    17581 agcgcatgaa cggactgctg ctggagactg tcaaacgcga ccggccggcg ctcgtgctgg
    17641 tcgttccatt cagggacgaa tttgatcctg ccgtgatgga cgacctcatg aaatgcacga
    17701 cgactgtcgc gtatttcttc gacgacatct ggcgcagaga atactcggaa ttctgggcgc
    17761 cgcacttcac gtatgtgaca acatcggatg tgaatggagt gcgccggttt agggaggcgg
    17821 gatccaacaa cgcggtctac tcgccgtttg cctgcaatca cgaaatcttc gtgaagaagg
    17881 accttgagaa gatctacgac gtagccttcg tgggtcaatt tcatcccaac agagcatggt
    17941 ggatcaggga gctcagaagg gctggcatta acatcgccgt ctggggccat ggctgggaca
    18001 cgggctcggt cggcgaggaa gatctcgtcc ggattttcaa tcaggccaga atcagtctta
    18061 atctctccaa ctcgaactcg ctggatctgc gttatgttct ggctctcaga aggccgatct
    18121 ccgagacgct acgcacgtgg aagcggaccg cgcgtgcgtt catgcgaggt gactcgaaaa
    18181 atcgagagca ggtcaaggga cgccatttcg agatcagtgc gtgcggtggg ttccaaatct
    18241 cttactatgt cgagggtctg gagcgccact atcaaatcgg cgaagagatt gccctttacg
    18301 cgtctccgga tgacttgatc gagacgatac gttactacct gaaacacgac gacgagcgag
    18361 aggcaattgc cctccgcgct tacgagcgca cctggagaga gcacacgatg gcgcgacgtt
    18421 tccgggatct gctcggacag actggtcacg cggaactgct cgtcgctccg tgattccggg
    18481 taccgagggg aaccaatggc ggcgcgacgc cggccaatgt taaaggagcg aatattgaca
    18541 aacactgaca aaactgccat ccgcaccaac gcctgcgagt attctcgtgt ttacgaaggc
    18601 gcggcccctc ggatttcgct ctggctacac atccgtgcga aggtccactc gctcaaacga
    18661 tggttctcac cgatcgacga cttcaactgg gacgactatc ctgcgcatta ccgggccgag
    18721 aacgagttca atcggcggtt tttcaccgac gatttgcgcg acgtcgaatt tctgttcacg
    18781 aacgggaaac tctatttgct tgcggactgc aagccgctca accagacgca ccgatgtgtt
    18841 tacgaggcga tctgtaacct tcccgggatc cgctcaatcg ccgaaatcgg agtcgggggc
    18901 ggacggtacc tcgcgaatct ccgtctgttg atggggaagg atgttgcgct ctccggttac
    18961 gatcgagcgc cattgcagat caagtttttt gaagagctat ttccagaggt gttccgagag
    19021 acgacgaccg gcgtcatgga ccttacggag gcatcgatca aagaagagtc cgtgccggac
    19081 cttgtctacg ccgttacagt gttgatgcat attcagcgac ccgacgctta tcgtaatgcg
    19141 ctgagcaatc tcctcgccag cggccgccac ttcgtgctcc tcctcgacca ctggaacgcc
    19201 catgattatt tcacggatct caccgcatac gccgcgagcg atccttccgc tcgactctac
    19261 acgtacgact ccggcgctaa cattgcggtt gtcgtcgcga ggaacggccg cacactagac
    19321 accccgtatg tcccgctgac cgatccggcg gcattgcaac gttacctgcg cccgctgtga
    19381 tggcctcagc gagatgagaa aagcagtccg gatgtctccg tcgtcatccc ccgactctga
    19441 accgcgcatg attacatcac ggatctcgcg tgacggcact ttggcgaagg cacgactcgt
    19501 cattgcaccg ccgtggcccg tcgggtaaag gcagtacaag ggcgggcaca ccacgaccat
    19561 gtcaaacgga agcgcgatcg gcggcggttg aaatatcgat ttgcacagct tttttcagta
    19621 cctgttctat ttctttgagg aacacggatc gttcgagctt tatcgaataa cgccgtcggg
    19681 ctatctggcg agagtggaac accgtgagac tctcgaacag tttcgaacca ccaactttct
    19741 ggcgcgtcga aaggcgaggc tgctctcatg agtcctgatt cgttctcggc aaaggttctc
    19801 gaatcctcga gcccgatgtt ttcggttgtg atcccgacgt tcaatcgtga gaaaaaggtc
    19861 gcgaaagctg tgcgaagtgt gctcgcgcag accttcacgg atttcgagat tgtcgtcgtg
    19921 gacgacgggt ccgatgagac ggcgaggcag ctgcaagacg agttcggcga tcgcgttcgg
    19981 tattttcgcg ggctggggca gcgtgtggcc ggtgcgagaa atctcgcgat cggtcatgct
    20041 ttgggagaat ggattgcctt tctggactcg gacgattggt ggtatccgac caagctcgag
    20101 cgctatgccg aagcagcaag agcgcatccg gagatcgggc tcttctactc gatgatggac
    20161 atggtcgacg atcgtgggcg gtatatccgc accctgccca tccgcacacg gcgcgacgta
    20221 tatcctgcgg tcctggaggg caacttcatc tttaactcga ctgttgcggt gaaaagagag
    20281 tgcctgagcc gggtcgggct gttcgatacg aagctgatcg gttgcgaaga ctgggagttg
    20341 tttgttcgag tgactcgcga gtttcccgcg ctgctgatcg acgagtcact ggtcgcgtat
    20401 gagcacagtt catctggaag cctgtcgagt aatcatgaag tgtgggtggc cgctcacgac
    20461 gaagtcattg cgaaatgcct agccgcggat acgacacttt cctctgcatg ggtccagaga
    20521 atacacgcgg gcgggcatta tgtgcaggcc aggatctacc ttggcgcagg agacgagtgc
    20581 catgcgttgg agaacttcag ggctgctttt cgacagaacc ggcgccgatg gcgcgcgttc
    20641 gtttacatga ttctcctttc ttggaagcca tccaggatga ttttgtcgcg tcgcgcgaag
    20701 gtttggctcc gtcttcctga agcgcggtga tccactgtga gactctttga accaccatat
    20761 cgcaagggac ctctgtattt tcgatcgtac agcgctctgg gcagtcttca tgcacatccg
    20821 gcactgcaat ggagtatcag gtggggcgca cggccgtgct caaccgtgcc ctgactgctc
    20881 tgataagtca acaacagcgt gagacgctac actagatgcg cgtgatcggc tgcgtctggt
    20941 tgaacgagcg cggggacgca gaatgctgat acaggacagt tggtcggcgt ggacctactc
    21001 tgcgcccgcg gactctagat gatgctgcgt gcaggcaagg cccgttggcg gacatgtgcg
    21061 agcgtcctcg cattgtgcat ggcgggagca gcggcatcta cgccggtggc cgggcaatta
    21121 cgatcaaaca tgctgttgat cagttgtctg cggcagcatg gcctcgaagg gatttgcccg
    21181 gccctcggcg acactcccag aggccgaata cacctgggtg ttgcagcagc gcgcgctggc
    21241 caagatgccg acgcaattcg gtacctcgca ccggcgttcc ggaatcacgc caggaacgac
    21301 ctggtgggct attttctggc gagagcgtac gcgcgctcag gcaactggca ggcggcaacg
    21361 ggtgtgtggc gacagacggg cgctgtcgag tatctgcgaa tggagagtgc gcgcggctcc
    21421 attgaggcga ccgaggcgct ggtcaaactc gatgaaggct catcgtggtc aataagattg
    21481 ggcgacatcc tttgggaata cggtcgtgtg ggcgaggcat gcgcggcgta cagaagagca
    21541 attgcgcttc cggcatccgc cagcttcgct gccgaacgcg cccgaggtcg attgtgcgag
    21601 tcaaacgaag attggcaggg agccttgaag cactacgaga ggagcattcg cctgctcatc
    21661 gacaagaaca cgctgtcagc gttcgacgcc
//
LOCUS       DATLVW010000831         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_570357, whole genome shotgun sequence.
ACCESSION   DATLVW010000831 DATLVW010000000
VERSION     DATLVW010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570357"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VNN25_24310"
     CDS             <1..968
                     /locus_tag="VNN25_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXH94722.1"
                     /translation="DLSYGTPDGVRFRVNVFKQRGQTGMVLRIIPAEIPNFEKLNLPA
                     TVQKLAEEERGLILVTGITGSGKSTTLAAMIDYINAHRTEHIMTVEDPIEFLHRDKKS
                     IVNQREIEVDTKSFSQALRSALRQDPDVILVGEMRDYETIETALTAAETGHLVLSTLH
                     TMDATETINRIISVFPPHQQKQIRLQLAAVLKAVVSLRLLPRADGLGRVPAAEVMIST
                     GYIRDCIENKEKTKLIKDAISAGTSQYGMQTFDQSLYVLYKNGLITLDEALRRASNPD
                     EFRLKLQGIQSTSDMAREEMDSKLAVTNDSDPFADESPFDFSNTV"
     gene            complement(984..1394)
                     /locus_tag="VNN25_24315"
     CDS             complement(984..1394)
                     /locus_tag="VNN25_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HXH94723.1"
                     /translation="MADTHPNPPLKVALFNGLRRRCPRCGEGQLFRRGLKTYERCSGC
                     NLLFQRDYGDTLMFMIITDRIPILFGIAALYFGIHPNGWASTVFFFAALSVPLLATLR
                     ERQGLALALDYVLRVKLSDPSDELHAASLPSHSA"
     gene            complement(1493..1837)
                     /locus_tag="VNN25_24320"
     CDS             complement(1493..1837)
                     /locus_tag="VNN25_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXH94724.1"
                     /translation="MLSDTRLPKPDTRRKAIYAAVKRIPRGRVATYGQIATIAGLDGH
                     ARQVGYALHDLPGGSDIPWHRVINARGEISPRSAGDSHELQRMLLEAEGVEFDLAGRV
                     ALNRYRWSGGKR"
BASE COUNT          401 a          628 c          653 g          357 t
ORIGIN      
        1 ccgacctctc ctacggcacg ccggacgggg tccgcttccg cgtcaacgtg ttcaagcagc
       61 gtggccagac cggcatggtg ctgcgcatca tcccggccga gatccccaac ttcgagaagc
      121 tcaacctgcc cgccaccgtg cagaagctgg ccgaggagga gcgcgggctc atcctggtca
      181 ccggcatcac cggctcgggc aagtcgacca cgctggcggc gatgatcgat tacatcaacg
      241 cccaccgcac cgagcacatc atgacggtcg aggatccgat cgagttcctt caccgcgaca
      301 agaaatcgat cgtcaatcag cgcgagatcg aagtggacac gaagtcgttc tcgcaggcac
      361 ttcgctcagc gttgcgtcaa gaccctgacg tcatcctggt cggcgaaatg cgcgactacg
      421 agacgatcga gacggcgctc accgccgccg agacgggcca cctcgtgctc tcgacgctgc
      481 acacgatgga cgcgaccgag acgatcaacc gcatcatctc ggtctttccg ccgcaccagc
      541 agaaacagat ccgcctgcaa ctcgctgccg tcctcaaggc ggtcgtctcg ctgcgtctgc
      601 tgccgcgagc ggacgggctt ggccgcgtcc cggccgccga ggtcatgatc tcgactgggt
      661 atatccggga ctgcattgag aacaaggaaa agacgaagct catcaaagac gcgatttccg
      721 ccggcacgtc gcagtacggc atgcagacgt tcgatcagtc gctgtacgtg ttgtacaaga
      781 acggtctgat cacgcttgat gaagcgctgc gccgggcctc gaatcccgac gagttccgcc
      841 tcaagctgca ggggatccag tcgacgtcgg acatggcccg cgaggagatg gattcgaagc
      901 tggccgtgac caacgacagc gatccgttcg cggacgaatc gccgttcgat ttctcgaata
      961 cggtatgagt ggttggttgc cggctacgcg ctgtggctgg gcaggctggc agcgtgcagc
     1021 tcgtcggatg gatcgctgag cttgacgcgc aggacgtaat cgagcgcgag cgcgagtccc
     1081 tggcgctccc gaagcgtggc cagcagcgga acggagagag cagcgaagaa gaagacggtg
     1141 ctggcccagc cgttcggatg aatcccgaag taaagcgcgg cgatcccgaa gaggatgggg
     1201 atacggtcgg tgatgatcat gaacatcagc gtgtcgccgt agtcgcgctg aaaaagaagg
     1261 ttgcaccccg agcaacgttc gtaagtcttg aggccgcggc ggaagagctg gccttcgccg
     1321 cagcgagggc atcggcgacg cagaccgttg aagagagcga ccttcagcgg aggattcggg
     1381 tgggtgtcgg ccacggtgct gacaacggct gcgcagctga agcgattccg cgttgatcgc
     1441 cagagagatt tttcacgcgg agacgcggag tacgcggaga atcatccatc gctcaccgtt
     1501 tgccgccact ccaccgatag cggttcagcg ccacacgccc ggccagatca aactccacgc
     1561 cttcggcttc gagcaacatt cgctgcaatt cgtgggagtc gccggcgctg cgcggggaga
     1621 tctcgccgcg cgcgttgatg acgcgatgcc aggggatgtc gctgccgccg ggcagatcgt
     1681 gcaatgcgta tccgacctgg cgcgcgtgac catcgagacc agcgatcgta gcgatctgtc
     1741 cgtacgttgc cacgcgtccg cgcgggatgc gtttcacggc cgcgtagatg gctttgcggc
     1801 gggtgtcggg tttcgggagt cgggtgtcgg agagcatgtg tcgattgtag ggggtgggga
     1861 gtggggtgtg gggagtggga gtcgtgagat taatctccga ctgccgaccc ccgataaccg
     1921 acgcccgata tctgtcgcgc acccttccac accccacgcc ccacacccca cccccatctg
     1981 ctaacatgca cacccttttg gtcacctaaa ggagtcgata tgcctcgcgc gtttgcttc
//
LOCUS       DATLVW010000832        28224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_404884, whole genome shotgun sequence.
ACCESSION   DATLVW010000832 DATLVW010000000
VERSION     DATLVW010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 28224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28224
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404884"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..537)
                     /locus_tag="VNN25_24325"
     CDS             complement(1..537)
                     /locus_tag="VNN25_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HXH94725.1"
                     /translation="MIREVLQAISFWAIPVLLVGIPLIGLIRGVKVYDVFIEGAKEGF
                     QVAIRIIPFLVGILVAIGMFRASGAMDLLTNALRPLMSRTIFPPELFPLAVLRTLSGS
                     GSLALTTDIIKRYGADSMLSRMAATLYGSSETLFYVLAVYFGAIGVKKTRHAIPSALV
                     GDLVAAIAAAAVCLWMFR"
     gene            complement(534..1163)
                     /locus_tag="VNN25_24330"
     CDS             complement(534..1163)
                     /locus_tag="VNN25_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HXH94726.1"
                     /translation="MLNYIWFGLMAIALIVAIFTGNAGALTKASVDSAKTAVEISLGL
                     VGIMTLWLGIMRVAEQAGLVAMLSRVLRPISRLLFPEVPADHPAIGAMVMSVSANMLG
                     LSNAATPLSIKAMEELQTLNEDKETASNPMVTFMVLNTAGVQLIPTTIIGVLAAAGSQ
                     APTAIIPTTLIATLCGAVAAVTTAKLLQRFFPTKLPPPTPDTPLPEVGP"
     gene            1363..3417
                     /locus_tag="VNN25_24335"
     CDS             1363..3417
                     /locus_tag="VNN25_24335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXH94727.1"
                     /translation="MTRNTFTLLALSTVVLVSGACQRETTSKQPASTSPRPSILLVTL
                     DTTRADSIGPEAVGIDTPSFNSLASHSRRFRWAYCAVPQTLPSHTSMMTGLYPAGHGL
                     HENGRYLSPAQALVAEKLHAAGYRTAAFVSAFALARRFGLARGFETYDDDMPQPRNER
                     TAEATTDRALALLSKPSAQPLFLWIHYYEPHFPYEPPEPFRTRYATKPYLGEIATMDQ
                     QLGRLVAAFQQRFGRDAAIIVVGDHGEGLGEHGEAQHGNLLYQATMHVPLLVAGPGVA
                     PGANDSPVSTRRIFHTILDWAGIDSANSLRGEISAPAASRTSEIVAAEAMKPFLDYGW
                     QPQVMAVDGRYKAIRAGRVEVYDVIADPAEAHDLGGGTQLTRGMRATLGEYPIPSLEA
                     ATTAEPPNVSDEERRKLASLGYVASTTKPLVRANAPRAADMTHLFSIEDQAAGLFVRE
                     QYTAAIPLLQRILTEDPYNLDAALRLGTCYSMIGRESEALATYQKAQSIAPQSQDVRQ
                     YLGLHYARGLEWQRAVPLLEQVVAESPDRVAALEGLAHVRERQGRLDDEVALLQRIYA
                     LRTPSPAELRHLGELAMQAGQTTAAIDAFEKARAVQGAAFANDLELGVLYLAANRLDD
                     ARTALDRVPPSHPGYAMALFKRAQVSVLMHEPDAAARIAAAREHANATTKELIARERL
                     FH"
     gene            complement(3477..6536)
                     /locus_tag="VNN25_24340"
     CDS             complement(3477..6536)
                     /locus_tag="VNN25_24340"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH94728.1"
                     /translation="MHLRRILCCLLVMFATTAVFAQQTGAFHGRVTASDGSALPGVTV
                     EARSSALPQPRVTVTDGNGDYRLPQLQPGSYTLQFTLAGMQTATRKADALLGQDMAAD
                     VKMAVGGVSENITVTAETTLVDRDTTAIQNGLGNAQIRALPVAQNYGDLQKLIPGAMV
                     TQDTVRGPSAGGSGQSNVYLFDGVNVTTPLFGTLGAEPATHDIAQVNVIKGGAKAVDF
                     DRAGGFLIDSVSKSGTNKYSGEVEYQVLNHTMIADQAGTVNTIFQEDRNWATASIGGP
                     IWSDHLFFYGSYYHPTRKRDNQANLTGPLPQFKVDRKEEFGKLTFTPTSSILINGSYR
                     NSTDVQTSGGGFGTKGAATTGTGNKGDLKIGTLEGSWVINPKSFGTFKFTDYKNPTIG
                     IPDHLSSAVPSTALGTHLDINNLDQLGLLTVPTVIATNPTQAAFVQPFIDKYGYTVAG
                     VKTGGGTVGFASLAHDDDDFYRKNGQAGYNYTLATKNMTHDLHIGYQQYTDSEDLFRE
                     SNGWGSISIPGGGTANNCPASACGGTAKTAFFQATFQQQTGGLVPVIHSEFRSRNIEL
                     NDSLRFNNWTFNIGVMASNDTLFGQGLTKADNVAGFVKSPGTKYEMHNVPYSQTIQPR
                     LGATWAYNGTDTVFASIAKYNPAANSDARAASWDRGLEQTLNAYFDASGNLLGVDPVK
                     SSSGKLFEAGIKPPKTQEYMIGTARQLGTRWSARMYGRYRYANNFWEDTPNNERVCLA
                     PFGAGGVCQASSFVSNAPASVPHVPYIPDLPAKLAAIGSGSTYVIANLDGAFTKYYEV
                     TTESEYTGDKFFLRGSYTWSHYYGNFDQDNSSFSTANDANLFIGSSNIADAAGRNVWD
                     NKYGDLRGDRRNIVKLYGAYTLPWQATTGAYFLYQSGQPYQIESFLPYNTITTSSSDT
                     ARYAEPAGRRRTPSYNQLDWNYTQGWALPKGLNLQLVLDWFNVMNHQTGYNYEARVGT
                     IGTCDPSKSTSCFYTGLTGPVAYLAHAPAPNSFYAPRRFQIAARLMF"
     gene            complement(6621..7592)
                     /gene="era"
                     /locus_tag="VNN25_24345"
     CDS             complement(6621..7592)
                     /gene="era"
                     /locus_tag="VNN25_24345"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXH94729.1"
                     /translation="MKKKENPVAEQESSATKHSRATKPRFGVVALVGRPNAGKSTILN
                     RILGHKVSIVSPKPQTTRNRIVGILNDPRGQIAFLDTPGIHRPLHKMNVRMMEHVRAS
                     LSEADVVAVMIDCSQPFGHGDEFVLEMMRDQKEKQPEAKRYAILNKIDLLKKNKLLPL
                     IQQYNETGLFDEIVPVSAMTGDGVEQLVNYFFAAVAAGEAIYSKEDFTTQPERFFAAE
                     IVREKLLAHTSEELPYTTAVAVERWEEEEEKNLIKIYATIVVERETQKPIIIGKRGDM
                     IKRIGTEARLELETILGARVFLDLHVAVHGAWREDERFLGELDWPLR"
     gene            complement(7564..8850)
                     /locus_tag="VNN25_24350"
     CDS             complement(7564..8850)
                     /locus_tag="VNN25_24350"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXH94730.1"
                     /translation="MASSLSHVTWIVLSAISGVLFLIFDAIRYFAQQISPVTLRRWSG
                     DQAGEGPGRWFHFDPQNLQLVSGSLLQITLIGAFVGTIRVLDGESAAFACGVSALAWL
                     VIVILWKFVLAFVPEDMGEMILKALIPFSHFFYFLFWPILFPLRRVFDRLDREEEEAH
                     EDEEVTDEEVQAYIDVGEEEGILEASEGKMIQSVVDFSDRVAHELMTPRIDVVAFDAT
                     HSFEELAKRFSESKYSRIPIYEESVDKITGVVHIKEVFESIVKGESKPVSELARAPYF
                     VSETKKVSELLREFQSEHIQVAVVVDEYGGTAGIITIEDVVEEIVGEIADEHEEEEAT
                     IVDLGGGEYLVSGQLRVEALEELLDAGLESDDYETIAGLIFTSTGRVPKAGIVVKKNG
                     YQFTVDRADRRRIYRVRVSKDPEWTNEDEEERESRG"
     gene            complement(8917..9387)
                     /locus_tag="VNN25_24355"
     CDS             complement(8917..9387)
                     /locus_tag="VNN25_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94731.1"
                     /translation="MPNWFYIFVNGLYLAGVSIWIGGGIVVTGVVIPEIFRLQPRHEA
                     VLLAVGVLRRFARIRVIALFLMLAGAGIKVLVWERHEIQPWMAVRWGAIVLMAWALVV
                     DLLRHRRLNALATSVTPQLPSDDPQREVLDIFRIYAEGIMRASLVAALIALLFS"
     gene            9445..10125
                     /locus_tag="VNN25_24360"
     CDS             9445..10125
                     /locus_tag="VNN25_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94732.1"
                     /translation="MLLATLLLAVTVAGSSPPASVPITTTLILRNGTHFDVEGPIREE
                     QGRVIFRTEGQLYSIPLADVDMDATRAAVTKAIVVTGDSGKKLKVSAPERERLLRELE
                     QNHTGKPSSSASGDRLPEPREAAAPSNPDEWSWRLAARAHEEAIRRAKENVELLRTRA
                     SALKQQIEQFFALGYKANQFTYQSSELEITQYQIPAAELEVTRAQRENDQFREDARKQ
                     GIMPGWLR"
     gene            10253..10906
                     /locus_tag="VNN25_24365"
     CDS             10253..10906
                     /locus_tag="VNN25_24365"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH94733.1"
                     /translation="MPSYAATVTEIEELAPSVVQITVAFDDDTFTFEPGQWVNFRFPD
                     GISRAYTIASAPHRTQTVQLCVRVGSGRGGKALTKLDAGAHVTIEGPYGDFLLPENDG
                     RPLIFMAGDTGIAPIRSIVLSMLANHDPRSICVLYEPDQRNILYAADFDPLARDGAIR
                     HESGKIETLIARNRKLIPDATVMAAGFDPFIDRVVDSLRESAIDTKNLISETFGGMP"
     gene            complement(11099..11611)
                     /gene="def"
                     /locus_tag="VNN25_24370"
     CDS             complement(11099..11611)
                     /gene="def"
                     /locus_tag="VNN25_24370"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXH94734.1"
                     /translation="MPVLPIVKYGDPVLRQPTQPVTEIDEPLQKLIDDMVATMYAAPG
                     VGLAANQVGVSKRLAVLDLSVGKKPGGLHILINPEKVEAEGSITEEEGCLSIPDFVEA
                     VTRPERVKVRYTDRNGEKREISGEGLMARALCHEIDHLNGNLYIDHLRGFKKERILKK
                     VAKLAKSGMW"
     gene            complement(11801..12550)
                     /locus_tag="VNN25_24375"
     CDS             complement(11801..12550)
                     /locus_tag="VNN25_24375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXH94735.1"
                     /translation="MADESESGASLLARKEYARAVPLLKRDLDKYQSNPRIRLQYADA
                     LAGAGEHLEAIAQYERTAKYYEDNGLTVQSIAVAKKAEKVRAQISAAQPQEATEGKSD
                     PLFSRPVPKSPLFEVLTEVERDALIREMDLESHDEGSVIISEGQPGASMYVIGSGEVK
                     VYTHGPGGSTLYLAKLGEGDFFGEVSMLTGKPRTATITASQRTELLRLDKEKLDNALA
                     KHPGILKVLNEFYQRRAEHTVEAMIESLKKK"
     gene            complement(12735..13523)
                     /gene="ybgF"
                     /locus_tag="VNN25_24380"
     CDS             complement(12735..13523)
                     /gene="ybgF"
                     /locus_tag="VNN25_24380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXH94736.1"
                     /translation="MRQLAAAILLALVLAGCASNRAAGEDAGPPSLVPPPDAAPAPTN
                     PATDARLAELQVSMTELLERLDVLNSRIAKLEAAEGESRVVVQPQTRAAEPVVAPRAL
                     EPVPVPRVAEAPALVSRPIPAPVHAPAPVSGPLRSAAIADSYRDALILFGKGRMPEAR
                     AALQRIFDAEPSGELADNALYWIGETYFAAGDYTNAMRSYERVTKEYPEQNKAPDAMF
                     KIGLTFVKTGDIGMARKTFEEVIRRYPYSTASSSAKLELKRVRY"
     gene            complement(13520..14338)
                     /gene="bamD"
                     /locus_tag="VNN25_24385"
     CDS             complement(13520..14338)
                     /gene="bamD"
                     /locus_tag="VNN25_24385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXH94737.1"
                     /translation="MKKTLTLIAILSIALAGCRHFFHRNANTGGSSATRSLRTTFDPA
                     LMKMPKEKVFQLGEDQFAKKKYQKARTYYSHVYETYPNDPLGRRALLRVADTYYEQGD
                     SVNLVEAQYKYRDFINRYPGSDRADYAMFQIAMCSYKQMERPDRDQQKTREALEKFND
                     VLKTYPKSPLRPDMDKRMQDVLDRLARHDHIIARYYIKRRSFNAAVQRLNNIVETYPN
                     YVERDGVFFDLGTALSGLGRTAEARLYFERVLSEFPQSVWAVKAKQKLGTMKTA"
     gene            complement(14480..15151)
                     /gene="rpe"
                     /locus_tag="VNN25_24390"
     CDS             complement(14480..15151)
                     /gene="rpe"
                     /locus_tag="VNN25_24390"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXH94738.1"
                     /translation="MTTKIRIAPSLLSADFSRLGEELRDIERGGADLVHLDVMDGHFV
                     PNLTIGPAVVKACRAQTTLPFDCHLMIAHPQQYIGAFLDAGANMISIHQETEPHLQRA
                     LRLIRDGGAKAGIAINPATPAESLSAAIEFCDFVLVMTVNPGFGGQKFIEPVVGKIRL
                     IEQMIRERGVAVDIEVDGGIDPSTTPLVVAAGATILVAGSAVFGQPDRKMAMEAIRKS
                     VSVTV"
     gene            complement(15148..15954)
                     /locus_tag="VNN25_24395"
     CDS             complement(15148..15954)
                     /locus_tag="VNN25_24395"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HXH94739.1"
                     /translation="MSEPRKHGFLDSVLFAALVVLVFGVSTYLCFTLFIRGRSVSTPN
                     LVGKSVKDATEVCTELGVSLVVDPEHQRNSDKIAAGSIVWQNRAPGATNLVKRGTDIH
                     VELSAGPLVLQVPDLDGQSARTALLRLGQQNLKLDNLAYVDSSLPPGIVAEEPPKGTV
                     VAAQSKVSLLVAVPVPPPSFVMPDLIDYPLDTVRPSLERRGLKVSNVKFEAYPGIADG
                     IIIRQFPLRGAEVSGRDPITVVVSRQEEGGIIEQPAAVGPPSAVPSTTNQ"
     gene            complement(15951..18416)
                     /gene="lon"
                     /locus_tag="VNN25_24400"
     CDS             complement(15951..18416)
                     /gene="lon"
                     /locus_tag="VNN25_24400"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXH94740.1"
                     /translation="MTQTNRKLPGSGNPDDWMDEEIPKSLPIIPLVSSVLFPNGVLSL
                     QVGIDRNVRLLKSLGEDQNLIAAFCQKTGDKENPRAEDLSQIGVLAAIIQRLPLSADR
                     YQVFLQGRERVELVHMLQNEPYFEGMLREVVPRPIPKSAKTDNLMNKAMSLFGKLVES
                     DPKYSNEILNILRMNMTEGPDTFADLLSSFVNFPLEEKQLLLETVNPIERLELLVAGV
                     QRDLGKATFDKELQRQIQTSIDRRDREGYLREQLRVIQDELGDSSSSDREADSYRSRV
                     EALPLAEEHKESLRREVNRLSQLSPSSSDYAVIKGHLDTVFQVPWAAKTEDQLDLVRA
                     EEILDERHHGLEKVKERVLEFLAVLKLKGDLKGPILCFVGPPGVGKTSLGAAIADALG
                     RKFVRMAVGGVTDESEIRGHRKTYIGAMPGKLIQSYIQVGVNNPLIMIDEIDKIGKDF
                     RGDPASALLEVLDTKQNNAFVDRYLEIPYDLSHTLFISTANMLDTIPSPLRDRMEVIR
                     LSGYTEPEKVEIAKKHLVPELLASHGLEPDQVTIDDDALFTVIRDYTGEAGVRNLERK
                     IATILRKIARKVASGEEQTHITVTSADLEPYLGLPSYEHEFAERHPEIGVTTGLAWTQ
                     FGGEIMFIEATRMAGSGRTTVTGQLGDVMRESVTAAYSYVRSKASDLEIDDKQFSEHD
                     IHIHFPAGATPKDGPSAGVAIATCIASVMGERPVRHDVAMTGEITLRGKVLSVGGVKE
                     KVMAAQRSNIKTVVLPEGNRKDLAEVPEDVKNGLHFVFAARVEDVWKETLIPLYIARP
                     SSRKYDEAEYRAEHQKSERPEQR"
     gene            18611..19591
                     /locus_tag="VNN25_24405"
     CDS             18611..19591
                     /locus_tag="VNN25_24405"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HXH94741.1"
                     /translation="MSDTATVQILADPKVKRADLHCHSRYSVFKYFRRANTRDCYNNP
                     EDVYRIAKERGMSYVTITDHDSIDGALYLLNKFPDMKDFFIGEEVETYFPETSQRIHI
                     GVWGLTELQHREIQRLRPDIRDLVPYLKSERLIFGVNHLFQNYRMKNVAARYIAELLE
                     MFDIFEVLNGAMASFHNKMVQQLVNSVQKGGRRASMIGGSDAHTLKYVAKVHTVSKGE
                     TVPEFLENIRAGDCFAWGSEMRFRELVADIYLLTLAYNREASADFMSQDYSVADKTMQ
                     LAGRLASIPAAISGIPAAITSLNYLKQIIVTKGISMRFEKLVQEIRPGLK"
     gene            19706..20056
                     /locus_tag="VNN25_24410"
     CDS             19706..20056
                     /locus_tag="VNN25_24410"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH94742.1"
                     /translation="MSDKPLVWLGSSRIDVRTFPAFVRRLTGFQLRRTQQGLDPDDWK
                     PMPSVGPGVREIRIHTEGAYRVFYIAAFKEAIYVLHSFEKKSSKTAARHLEIGRQRFR
                     ALVQMRQHEKKRNR"
     gene            20034..20345
                     /locus_tag="VNN25_24415"
     CDS             20034..20345
                     /locus_tag="VNN25_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH94743.1"
                     /translation="MKRKGTVRVTPSSGNVFRDLGFSPKEAEHLSVRADLLIQLQKVI
                     VSQGLKQAEIAKILHVTQPRVSDLLRGRIDLFSTDTLLDMLASLGAIVRLVVKAPRKV
                     A"
     gene            complement(20392..21240)
                     /locus_tag="VNN25_24420"
     CDS             complement(20392..21240)
                     /locus_tag="VNN25_24420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94744.1"
                     /translation="MSDAKEPKRKRCFVITPIGDPGSQVRRATDGLVTNVIRPTLQAN
                     GFDVAAAHEISAPGSITMQVIDRLLNDELVVANLSGLNANVMYELAVRHAVRLPVITL
                     AEEGTELPFDIADERTIFFTNDMHGVDEVRPKLEAAMNLALAETAPDNPIYRAREAKV
                     MRDVVAKNDTEKYVLDRLEAIEAAISRLASPPTVSHELRDPRVLRGRPQHKIKLRGTD
                     EAWENARRAFYDTDCVSSIKMMSNDGIFDVALTLRGPMNRAAVHRIARDAGLEVMPRE
                     PSLPAA"
     gene            complement(21248..23059)
                     /locus_tag="VNN25_24425"
     CDS             complement(21248..23059)
                     /locus_tag="VNN25_24425"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH94745.1"
                     /translation="MRRLRAIAIASLAIVFAVPAQSEVPLPAVLQVLGSVTNSARPVG
                     NALVIALNLNNLAAIQTFTSSDGSFSLPQLPAGVYRIIAMKYGFTPAIETIVPTKKEH
                     RLKLRLETEKQSKGTNQEMWEIRGSLPPDVLRQIDMVMAPPPDPIGPGALAYEVPRFK
                     GEMVSMTGMSAGTTTSPSGPAFAQTALGLQSRVGDNWQIGFHGNLHRVEDPTDNESFG
                     TPVAASSAAAIEIRSSPTDSYRLTSTRSWWRYRDEIPSESQADIRSHDFEWAHGDSHV
                     QVRYLAQQNLFVASPGSELIEIRGDTTVFQTPRNDLGVSLRVTQETSRTTAGAVAAPT
                     LRTADVTANAKFELVPAFTVDYGMSSRVGLYGTEWAPRSGAEWKVGKETALVVSGMYK
                     VVDSEHQNTLPSVVVWTDDSGQLPRYAYSVGIVSGDNSREHFSVVGSVSAVDTPLRIV
                     FTDGLEHFWDGLYIDAGDIRRDVRVAFRKELGRRFLLDVSSMAGTASPAHPGVTSRGG
                     EKLYLAGDVESTFSPTRTTLAVSYRQINQPQPNGGDAYRTHRVDLRLAQSLHLPLDLK
                     VLLGIELARAENSRILLDALDADGVTRRYLGGLAVNF"
     gene            23235..24668
                     /gene="pyk"
                     /locus_tag="VNN25_24430"
     CDS             23235..24668
                     /gene="pyk"
                     /locus_tag="VNN25_24430"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXH94746.1"
                     /translation="MRRTKIIATLGPACGSEDVILRLLTSGVDVFRMNFSHGTPTDRL
                     RLIEIIRRVATDHGKYIPILGDIQGPKLRIGEVDGVIHLQPGQMFTITTEAVAGNGSI
                     VSTPFTPLPREVQIGQRILINDGLVELVVAAVDATKVLTRVVHGGPISSKKGMNFPDT
                     ELTIPAITEKDREDVAFAVANQLDYIAASFIRRRSDIVELRSLLRDLGGDEINIIAKL
                     EKSQAIDALEDILDVSDGVMVARGDLGVELPPEDVPIVQKRVLQKAAVWGRFAITATQ
                     MLESMTSNSRPTRAEASDVANAIFDGSDAVMLSAETASGAYPVEAVQMMARIIMAAEQ
                     NRQFQMAGGRDPFRVSTETDEFTDALAGAANYAAQQLDAKFIIVFTQTGFAARLMSKF
                     RPKQPIIALTPSTWVARRMNVLWGVQPFVLREVGEYHEQIVERVDEFLISRDIVVPGD
                     RLVILMGSPVYQRAKTNLLRVHRVRES"
     gene            complement(25250..26287)
                     /gene="aroF"
                     /locus_tag="VNN25_24435"
     CDS             complement(25250..26287)
                     /gene="aroF"
                     /locus_tag="VNN25_24435"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXH94747.1"
                     /translation="MLVVMEAGARPDQIEAVVRHIEKMGLKAHAIPGANRTAIGITGK
                     PGTADPEVLENYPGVKEVIRVSHAYKLVSREARPDDSVINVGGVDVGGNGIIVVGGPC
                     AVESLDQSRAIAEGIRNAGGQLFRGGAYKPRTSPYSFQGLGEPGLEILARIREEFDLP
                     IVTEAIDNEALALVDQYADCIQIGARNMQNFSLLKAAGRARKPILLKRGMSATLEELL
                     LSAEYIMAEGNYNVILCERGVRTFADHTRNTLDLSIIPAIKRISHLPVLVDPSHGTGK
                     RNKVAPMSRASIAAGADGVLIEVHHKPEEALSDGPQAILPEAFAVIVRECDAIARVVG
                     RSLQPALAATV"
     gene            26339..27055
                     /locus_tag="VNN25_24440"
     CDS             26339..27055
                     /locus_tag="VNN25_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94748.1"
                     /translation="MKKRMIVAVLAAVVAIPAFAASYWVVLKDGTRYEAKSKWTVVNG
                     KAVLNLVNGTVLNLDPNVINVAKSEEMTRLGGGDLFGVEQIPVTSTTKTSQLGATIKL
                     HKLPPAQAPGAAAPVEATIPVAPGALLGTDVLGKFERAYENVGIFEHKLIATGPHSLR
                     ADLTADTEDKVFNTLSATAFLMVRNAGITGVQIDYADIFMKTTTGAAAGRFHLTHDEA
                     QAIESKTITPQSYFVLKVLF"
     gene            complement(27245..>28224)
                     /locus_tag="VNN25_24445"
     CDS             complement(27245..>28224)
                     /locus_tag="VNN25_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94749.1"
                     /translation="PHSPLPAPRERILIRRLLILAAVVFIGVAAYSRLDLLYSHKFFD
                     NTGRAQWIWPVHRMAEGDPVAFFATYDFDLPPNRNFTRIKMLGDPEYTLYFNGVAVGG
                     RRVGDGNQALDTYDVSNLAHDHGNRMVVAVRSANGVGGLIAAVDLTREFANFIVTGSD
                     WHIVQRWHDDLLVRDPPAGEIRRPQLLGRPPARRWNYLTQKDAAFFAPTKQVIGAQQT
                     FDFKTALPEIAVIGGTAVMISRKTAATAYDFGTVTSGRVRLTIRKVENGPRNVTVRFA
                     YERAQLFTVDEPVEQFVFAAGERSLLDEEIRNFRYITVYGGAATVDVLR"
BASE COUNT         5277 a         8643 c         8666 g         5638 t
ORIGIN      
        1 ctaccgaaac atccagaggc aaaccgcggc agcggcgatc gccgcaacga gatcaccgac
       61 caacgccgac gggatcgcgt ggcgcgtctt cttcacgccg atcgccccga agtagacggc
      121 cagcacgtaa aagagcgtct cgctgctgcc gtagagcgtg gcggccatgc gcgagagcat
      181 tgagtcggcg ccgtagcgct tgatgatgtc cgtcgtcagc gccagtgagc cgctgccgct
      241 gagcgtgcgg agaacggcca gcgggaagag ctccggcgga aagatcgtgc ggctcatcag
      301 cgggcggagg gcgttcgtca gcaggtccat cgcgcccgag gcgcggaaca tgccgatggc
      361 gacgaggatg ccgacgagaa acgggatgat gcggatggcg acctggaatc cttccttcgc
      421 gccttcgatg aagacgtcgt agaccttcac gccgcgaatc aaaccgataa gggggatgcc
      481 gacgagcagg accggaatgg cccagaacga gatggcttgg aggacttcgc ggatcacggg
      541 cccacctcgg ggagtggggt gtcgggagtt gggggtggga gctttgtggg gaagaagcgc
      601 tgcaatagtt tcgcggtcgt cacggcggct actgcgccgc agagtgtggc gatcagcgtc
      661 gttgggatga tcgcggtcgg tgcctgagag ccggcggccg ctaagactcc gatgatcgtc
      721 gtcgggatga gttgcacgcc ggcagtgttg agcaccatga acgtgaccat cgggttcgag
      781 gccgtctcct tgtcctcatt cagcgtctgc agctcttcca tcgccttgat gctgaggggt
      841 gtggcggcgt tgctcagtcc cagcatgttc gcggagacgc tcatcaccat cgccccgatc
      901 gccgggtgat cggcaggcac ctcggggaac agcagccggc tgatcggacg cagcacgcgg
      961 ctgagcatgg ccacgagccc ggcctgctcg gcgacacgca tgatgccgag ccagagcgtc
     1021 atgatgccga cgaggccgag ggagatttcg acggcggtct tcgccgagtc gaccgaagct
     1081 ttcgtcagcg cgccggcatt ccccgtaaat atggccacga tgagcgcaat cgccatcagg
     1141 ccgaaccaga tgtaattcag catgacggcg attgtagtgg agactcagcg cgcggccggt
     1201 tggtaatggg tcaatttgaa aacccggtca ccacagagac acagaggtca cagagtccac
     1261 ctctgtgttc tctgtgtctc tgtggtcgcc tttgcttcgg caccacgata agcagtccaa
     1321 attgacccac gactggccgg ttcgcgtgtt agaactaccg cgatgacgcg caacacgttc
     1381 actctcctcg ctctctccac tgtcgttctc gtttccggcg cgtgccagcg ggagacgacg
     1441 tcgaagcaac cggccagcac ttcgcctcgt ccatcgattc tgctcgtcac tctcgacacc
     1501 acgcgcgccg actcgatcgg tcccgaggcc gtcggcatcg acacgccgtc gttcaattct
     1561 ctggcgtcgc acagccggcg ctttcgctgg gcgtattgcg cggttcctca gacgctcccc
     1621 tcccacacgt cgatgatgac cggcctctat cccgctggac acggtctgca cgagaacggg
     1681 cgctatctct cccctgctca ggccctggtc gcggagaagc ttcacgcggc cggttaccgc
     1741 accgcggcgt tcgtctccgc gttcgcgctg gcccggcgat ttggactggc gcgcggattc
     1801 gagacctatg acgacgacat gccccagcca cgcaatgaac ggacggccga ggcgaccacg
     1861 gaccgtgcgc tggcgctttt gtcgaaaccg tctgcgcagc cgctcttcct ctggatccac
     1921 tactacgaac cccacttccc ctacgagccg cccgagccct tccggacccg ctatgccacg
     1981 aagccctacc tcggcgagat cgccacgatg gatcagcagc tcggccgtct cgtggccgcg
     2041 tttcagcagc gcttcggacg tgacgccgcg atcatcgtcg taggcgatca cggcgagggt
     2101 ctgggcgagc atggcgaagc gcagcatggc aacctgctct accaggcgac gatgcacgtg
     2161 ccgctgctcg tggccggacc gggcgtcgca cccggagcca atgattcacc cgtcagcacg
     2221 cggcgcatct ttcacacgat cctcgactgg gccggcatcg attcggcgaa cagccttcgt
     2281 ggggagatca gtgcgccggc ggcgtcgcgc acttctgaaa tcgtcgctgc tgaggcgatg
     2341 aagccattcc tcgattacgg ttggcagccg caggtcatgg ctgtcgacgg ccgctacaaa
     2401 gcgatccggg ccggacgtgt cgaagtctac gacgtgatcg ccgatcccgc cgaggcgcac
     2461 gacctcggcg gaggcacaca actgacgcgt ggcatgcgcg cgacactggg cgagtatcca
     2521 attccttctc tcgaagccgc gaccacagcg gaacctccca atgtcagtga tgaagagcgc
     2581 cgcaaactgg ccagcctcgg gtacgtcgcc tcgacgacga agccgctcgt gcgcgcgaac
     2641 gcgccacgtg ccgcggacat gactcacctc ttctccatcg aagatcaggc ggctggactc
     2701 ttcgtgcgcg aacaatacac ggcggcgatt cccctgctcc agcgcatcct cacggaagac
     2761 ccatacaacc tcgatgcggc gctgcggctc ggtacctgct attcgatgat cggacgcgag
     2821 agtgaggctc tggccacgta tcagaaggcc cagtcgatcg cgccgcaatc gcaggacgta
     2881 cgccagtacc tcgggcttca ttacgcgcgc ggcctggagt ggcagcgcgc agttcctttg
     2941 ctcgagcagg tagtggccga gtctcctgac cgcgtcgccg cgctggaagg cctggctcac
     3001 gtccgcgaac ggcaggggcg cctcgacgat gaagtggcgc tccttcaacg catctacgca
     3061 ctccgcacgc cctctccggc cgagcttcgg catctcggtg agcttgccat gcaggctggc
     3121 cagacaaccg ccgccatcga tgcctttgag aaagcgcgcg ccgttcaggg agcggcgttc
     3181 gccaacgacc tcgagctcgg cgtgctctat ctcgccgcga acaggctgga cgacgcccgc
     3241 accgcgctcg atcgcgtgcc gccttcgcat cccggttacg caatggcgct cttcaagcgc
     3301 gcccaggtca gcgttctgat gcacgaacct gatgcagccg cacgcatcgc tgcggcccgc
     3361 gagcacgcga acgccaccac gaaagagttg atcgcccgcg agcgtctctt ccactgaacg
     3421 agaaaagagc ccggcgtcgc cgccgggctc tttcattcaa ctgcctgtag aagaagctag
     3481 aacatcagcc gtgctgcgat ctggaagcgc cgcggggcat agaaagagtt cggcgccggc
     3541 gcgtgcgcga gataagcgac tgggccggtg aggccggtat agaaacagct cgtgctcttg
     3601 ctgggatcgc aagtgccgat cgtgccgact cgggcttcgt agttatatcc ggtctgatgg
     3661 ttcatcacgt tgaaccagtc gagaactagc tgcagattca gacctttcgg aagagcccaa
     3721 ccctgggtgt agttccaatc caactgattg taggagggcg ttctgcgccg gccggcgggt
     3781 tctgcgtagc gagcggtgtc gctcgaggag gtcgtgatgg tgttgtacgg caggaagctc
     3841 tcgatctgat acggctggcc tgactggtag aggaagtacg ctcctgttgt ggcctgccat
     3901 ggcagcgtgt acgcaccgta gagcttcacg atattgcggc ggtcgccgcg aagatcgccg
     3961 tacttgttgt cccagacgtt gcggccggct gcatcagcaa tgttggaaga cccgatgaaa
     4021 aggttcgcgt cattcgctgt gctgaagctc gagttgtcct ggtcaaagtt cccgtagtag
     4081 tggctccagg tgtacgagcc acgaaggaag aacttgtccc ctgtgtactc agactcggtt
     4141 gtgacttcgt aatacttggt aaacgcgccg tccagattgg cgattacata cgttgaaccg
     4201 ctgccaatcg cagcgagctt ggccgggagg tccggaatgt acggcacgtg cggaacactg
     4261 gccggcgcgt tggagacgaa cgacgatgcc tggcaaacac cgccggcacc gaacggcgcg
     4321 aggcaaacgc gctcgttatt cggcgtgtcc tcccaaaaat tgttcgcata gcgatagcgc
     4381 ccatacatgc gggccgacca tctggtgccc aactgtctgg ccgtaccaat catgtattcc
     4441 tgcgttttcg gaggcttgat gccggcttcg aacaacttac cggaagacga cttgacgggg
     4501 tccacgccca gcaggtttcc gctggcgtcg aagtacgcat tgagcgtctg ctcgaggccg
     4561 cggtcccagg aggccgcgcg cgcatcggag tttgcggccg gattgtattt cgcgatgctc
     4621 gcgaagaccg tatccgtacc gttgtatgcc catgtcgcac cgaggcgcgg ctggatggtc
     4681 tggctgtacg gtacgttgtg catctcgtac ttcgtgcctg gcgacttgac gaatccggcc
     4741 acattgtcgg ccttcgtcag tccctgacca aagagggtgt cgttgctggc cattacgccg
     4801 atgttgaacg tccagttgtt gaaccggagg ctgtcattca gttcgatgtt ccgcgagcgg
     4861 aactcagagt gaatcacggg gacgagccct cccgtctgtt gctggaaagt cgcctggaag
     4921 aaggccgtct tcgctgtgcc gccgcaagca gacgccgggc agttgtttgc tgttccgccg
     4981 ccgggaatgc tgatggagcc ccagccattc gactcccgga ataggtcttc agagtcggtg
     5041 tactgctgat atccgatgtg aagatcgtgc gtcatgttct tcgttgcaag agtgtagttg
     5101 tagccggcct ggccgttctt ccggtaaaag tcatcatcgt cgtgggccag ggaagcaaaa
     5161 ccgacagtgc cgccaccggt cttcacgccg gcgaccgtgt agccgtactt atcaatgaat
     5221 ggctgtacga acgcagcctg cgtcggattc gtggcaatga ccgtaggaac ggtcagaagc
     5281 ccgagctgat ccagattgtt gatgtcgaga tgcgttccga gcgcagttga cggaactgca
     5341 ctggagaggt gatcgggaat cccgatcgtc gggttcttat agtcggtgaa tttgaatgtt
     5401 ccgaagctct tgggattgat gacccacgaa ccctcgaggg tgccgatctt caggtcacct
     5461 ttgtttccgg tgcccgttgt tgctgcgcct ttggttccaa agccaccgcc agacgtttgc
     5521 acgtccgtcg agtttcgata gctgccgttg atgaggatcg aagaggtcgg cgtgaacgtc
     5581 agttttccga actcttcttt acggtccacc ttgaactgcg gaagcggacc agtcagattg
     5641 gcttgattgt ctctcttacg agtggggtgg tagtacgagc cgtagaaaaa gagatgatcg
     5701 gaccagatgg gtccaccgat gctggctgta gcccagttac gatcttcctg gaagatcgta
     5761 ttcacggttc cggcctggtc ggcaatcatc gtgtgattga gaacctggta ctcgacctcg
     5821 ccggagtact tattcgttcc cgacttgctc acggagtcga tcaggaaccc gcccgcgcga
     5881 tcgaagtcga ctgctttcgc gccgcctttg attacgttga cctgggcgat gtcgtgggta
     5941 gcaggctcgg ctcccagcgt gccgaacaga ggtgtcgtca cgttgacgcc gtcgaaaaga
     6001 tagacgttcg actggccgct gccgccggcg ctcggaccac gaaccgtgtc ctgagtcacc
     6061 atggcgcccg ggatgagctt ctgaagatcg ccgtagttct gcgcgaccgg gagagcgcgg
     6121 atctgggcat tgcccaggcc gttctggatc gcggtcgtat ctcgatcgac gagagtggtc
     6181 tcggcggtca ccgtgatgtt ctcggagacg ccacccacgg ccattttcac gtccgcggcc
     6241 atgtcctgac cgagaagggc atcggccttc cgcgtcgctg tctgcattcc ggcaagcgtg
     6301 aattgcaggg tgtatgaccc cggctgaagc tggggcaggc ggtaatcgcc gttgccatcc
     6361 gtcacggtga cgcgtggctg cggcagcgca ctggatcgcg cttcaacggt aactccgggc
     6421 agcgccgatc cgtctgaagc agtaactctc ccgtggaatg cgcccgtctg ctgggcaaag
     6481 accgcggtcg tggcaaacat caccaggaga caacacaaga tcctgcgaag atgcatagat
     6541 tcccctcttt ttcctgaacc gttaggaccg atttgggcgg attctacacc ccgcatcgcc
     6601 cgtagcggga aggcaaaaaa tcagcgcagc ggccaatcca attcgccgag gaaacgctcg
     6661 tcctcacgcc aggcgccgtg gacggcgacg tgaaggtcga gaaagacgcg cgcgccgagg
     6721 attgtctcca gctccagccg cgcctcggtg ccgattcgct tgatcatgtc gccgcgcttg
     6781 ccgatgatga tcggcttctg tgtctcgcgc tcgaccacga tcgtcgcgta gatcttgatg
     6841 aggttctttt cctcttcttc ttcccagcgc tcgactgcca ctgccgtcgt gtagggcagc
     6901 tcttccgatg tgtgcgcgag cagcttctcg cgaacgatct ccgcggcgaa gaagcgctcc
     6961 ggctgcgtgg tgaaatcttc cttcgagtag atggcctcac ctgccgccac ggctgcgaag
     7021 aagtagttga cgagttgctc taccccatct cccgtcatcg ccgaaaccgg gacgatctca
     7081 tcgaacagcc ccgtctcgtt gtattgctgg atgagcggca gcagcttgtt cttcttcagc
     7141 aggtcgatct tgttgaggat tgcgtagcgc ttcgcctcgg gctgcttttc tttttgatcg
     7201 cgcatcatct cgaggacgaa ctcgtcgccg tgcccgaacg gctggctgca gtcgatcatc
     7261 accgcgacca catcggcctc ggacaaactt gcccgaacgt gctccatcat ccgcacgttc
     7321 atcttgtgca acggccggtg aatcccgggc gtgtcgagaa acgcgatctg gccgcgagga
     7381 tcgttgagga tgccgacgat gcggttgcgc gtcgtctgcg gcttcggcga gacgatcgag
     7441 accttgtgcc cgaggatgcg gttcagaatc gtcgacttgc cggcgttggg acggccaacc
     7501 agcgcgacca ctccgaagcg cggtttcgtt gcacggctgt gtttcgtcgc tgagctttcc
     7561 tgctcagcca cgggattctc tttcttcttc atcttcgttc gtccattccg gatctttcga
     7621 gacccgcact cggtagatcc gccgccggtc cgcgcgatcg acggtgaact gatagccgtt
     7681 cttcttgacc acgataccgg ctttcggcac gcggccggtc gacgtgaaga tgagccccgc
     7741 gatcgtctcg tagtcgtcgc tctccaagcc ggcatcgagc agctcttcca gcgcctcgac
     7801 gcgcaactgc ccgctgacga ggtattcgcc gccgcccagg tcgacgatcg tcgcttcctc
     7861 ttcctcatgc tcgtccgcga tctcgccgac gatctcctcg acgacatcct cgatcgtgat
     7921 gatgcccgcc gtgccgccat attcatcgac gacgaccgcg acttgaatgt gctcgctctg
     7981 aaactcgcgc agcaactccg agaccttctt cgtctcgctg acgaaatacg gcgcgcgcgc
     8041 caactccgag accggcttgc tctcgccttt gacgatggac tcgaacactt ctttgatatg
     8101 aacgacgccc gtgatcttgt ccacgctctc ttcgtagatc gggatgcgcg agtacttcga
     8161 ttcgctgaag cgcttcgcca gctcctcgaa cgagtgcgtg gcatcgaacg cgaccacatc
     8221 gatacgcggc gtcatcagct cgtgggccac gcggtcgctg aagtcgacga ccgactggat
     8281 catctttcct tcactggcct cgaggatgcc ctcttcttcg ccgacgtcga tgtaggcctg
     8341 cacctcttcg tcggtcactt cttcgtcctc gtgcgcctct tcttcttccc ggtcgagccg
     8401 gtcgaacacg cggcgcagcg gaaagaggat cggccagaac aggaagtaga agaaatgcga
     8461 gaacgggatg agcgccttca ggatcatctc ccccatatcc tcggggacaa aggcgagcac
     8521 gaatttccag aggatgacga tgacgagcca ggccagtgcg gagacgccgc aggcaaacgc
     8581 ggccgactct ccgtcgagca cgcggatcgt tccgacgaac gcgccgatca gcgtgatctg
     8641 cagcagcgag ccgctgacca gttgcagatt ctgcgggtcg aagtggaacc agcgtcccgg
     8701 cccttcccca gcctgatcgc ccgaccagcg gcgcagcgtg actggactga tctgctgcgc
     8761 gaagtagcgg atggcgtcaa agatcaggaa cagcactccg ctgatcgcgc tgagaacaat
     8821 ccaggtgacg tgcgagagac tactggccat tggtctggcg gcggtgttga ggcttgccgt
     8881 gtgggggcgt gaacatgggt gtaacggcgg attctatcaa ctgaacagaa gcgcgatgag
     8941 cgcggcgacc agcgacgcgc gcatgatccc ttccgcgtag attcgaaaga tgtcgagaac
     9001 ctcgcgctgt ggatcgtccg aagggagctg cggagtaaca ctggtcgcga gggcgttcaa
     9061 ccgcctgtgc cgcaacaggt cgacgacgag cgcccaggcc atcagcacga tcgcgcccca
     9121 acgaacagcc atccacggct ggatttcatg ccgctcccac accagaacct tgattccggc
     9181 gccggccagc atcagaaaaa gcgcaatcac ccgaattctc gcgaaccgcc ggagcacccc
     9241 cactgcgagg agtacagcct cgtggcgcgg ctgcaaacga aagatctccg gaatgaccac
     9301 gccggtcacg acgatgccgc caccgatcca aatcgatacg ccagccaggt acaggccgtt
     9361 gacgaagatg tagaaccagt tcggcacgga gcgctgatca taacgtttgc cggccgttaa
     9421 cggagatcag gcggataatt agccatgctt ctcgccaccc tcctgctggc cgtcaccgtc
     9481 gccggctcat ccccgcccgc atctgttcca atcacgacaa cactcatcct gcgcaacggc
     9541 acgcacttcg acgtggaagg tccgatccgg gaggaacaag gacgggtcat cttccgcacc
     9601 gagggacagc tctattcgat cccgctcgca gatgtcgata tggatgcgac gcgggcggcg
     9661 gtgacgaagg cgatcgtcgt aacgggagac tccggcaaga agctgaaagt ctccgcaccg
     9721 gagcgggagc gccttctgcg ggagctggag cagaaccaca ccggcaagcc gtcatccagc
     9781 gcttccggtg accggcttcc ggaacctcgc gaagccgctg caccttcgaa tcccgatgaa
     9841 tggtcgtggc gcctcgctgc acgcgcgcac gaggaagcga ttcgccgggc gaaggagaac
     9901 gtcgagctgc ttcgcacccg cgcctcggca ctcaaacagc aaatcgaaca gttcttcgcc
     9961 ctcggctaca aggcaaatca gttcacgtat cagtcgagcg agctggagat cacgcagtat
    10021 cagattcccg ccgcggagct cgaagtcacc cgcgcacaac gcgagaacga ccagttccga
    10081 gaggacgcgc gcaagcaggg aatcatgcct ggatggctgc ggtaaaaccg ttttcaccac
    10141 agagacacag aggacacaga gatacggatt caacctctgt gtcctctgtg cctctgtggt
    10201 tcatgctttt cgaccccgac tcacgacccc cgcgcccaga tatactttcc ctatgccctc
    10261 ctacgccgca accgtgacgg agatcgaaga gctggcgccg agcgtggtgc agatcacggt
    10321 cgcattcgat gacgacacgt tcaccttcga gccggggcaa tgggtgaact tccgcttccc
    10381 ggatggcatc tcacgcgcct acacgatcgc gtcggcgcct catcgcacac agacggtgca
    10441 actctgcgtg cgcgtcggat cggggcgggg cggcaaggcg ctaaccaaac tcgacgccgg
    10501 cgcgcacgtc acgatcgaag gtccctacgg cgattttctc cttcccgaga acgatggccg
    10561 cccgctcatc ttcatggccg gagacaccgg cattgccccg attcgcagca tcgttctctc
    10621 gatgctcgcc aatcacgatc cgcggtcgat ctgcgtgctt tacgagcccg atcagcgcaa
    10681 catcctctac gcagccgact tcgatcctct cgcccgcgac ggcgccatcc gccacgagag
    10741 cggaaagatc gaaacgctca tcgcgcgcaa ccgcaagctc atcccggatg cgacggtaat
    10801 ggctgccggc ttcgatcctt tcatcgaccg cgtcgtcgat tccctgcgcg aaagcgccat
    10861 cgatacgaag aatctgatta gcgagacgtt tgggggtatg ccgtaggttg cgggagtcgc
    10921 gcgtcgggag tcgggtgtcg gagatttagg ttcgggagtg ggcgcggccc cggcacttgc
    10981 accagaacga aactccgagc atctgcgaag cgtcgactca atgcgaagcg tcgactcaag
    11041 aggcgggact ttgactcatc tccgaccccc gactcccgaa ccgcgactcc cgcaaaactc
    11101 accacatccc cgacttcgcc agcttcgcaa ccttcttcag gatccgctct ttcttgaagc
    11161 cgcgaagatg atcgatgtag aggttgccgt tgaggtgatc gatctcgtgg cagagggccc
    11221 gggccatgag tccttcgcct gaaatctcgc gtttctcgcc gttgcggtct gtgtagcgga
    11281 ccttgacgcg ctcggggcgg gtgaccgctt caacgaagtc gggaatcgac aggcagccct
    11341 cctcctccgt gatcgatcct tccgcttcga ctttctcggg attgatgagg atatgcaggc
    11401 cgcccggctt cttgccgacg gaaagatcga gcaccgccag ccgcttcgac acaccgacct
    11461 gattcgccgc cagtcccacg ccgggcgcgg catacatcgt ggccaccatg tcgtcgatca
    11521 gcttctgcag cggttcatcg atctcggtca ccggctgtgt tggctggcga agaaccggat
    11581 cgccgtactt gacgatggga agaacgggca aggttgtgtg ctcctgttat cgggagattg
    11641 gaggccagag atcagagatc aggcggcagg taacgtcggg cgctgatcgc tggtctctcg
    11701 tttccggtca tttccttctg gtagaaacca gcgatcagag cagatcgtga agaaactggc
    11761 tcatgtcctg atctcttgtc tctggtcgct aatctctgct tcacttcttc ttgaggctct
    11821 cgatcatcgc ttcgaccgtg tgctcggcgc gccgctggta gaactcgttg agaactttga
    11881 gaatccccgg atgttttgcc agcgcgttat ccagcttctc tttgtcgagc cgcagcagtt
    11941 ccgtacgttg tgatgcggtg atcgtggctg tgcgaggttt gccggttaac atgctcactt
    12001 cgccgaaaaa atccccttcg ccgagcttgg ccaggtagag cgtggagccg cccggcccat
    12061 gcgtgtagac cttcacttcc ccgctgccga tgacgtacat cgacgcgccg ggctgcccct
    12121 cgctgatgat gacgcttccc tcgtcgtggg attcgagatc catttcgcgg atgagggcat
    12181 cgcgctccac ttcggtcagg acttcgaaga gcggcgactt cggcacggga cgcgagaaca
    12241 gcggatcgct ctttccttcg gtcgcctctt ggggttgcgc tgcgctgatc tgggcgcgca
    12301 ccttctctgc cttcttcgcc acggcgatcg actgcacggt gagaccgttg tcctcgtagt
    12361 acttcgccgt gcgctcatat tgcgcgatcg cttcgaggtg ctcgcccgct cccgcgagcg
    12421 cgtcggcgta ctgcagccgg atgcgaggat tggactggta cttgtcgaga tcgcgcttga
    12481 ggagggggac ggcgcgtgcg tactccttac gcgccagcag cgatgcgccg ctttcagatt
    12541 cgtcagccat gcgtggattg tatcgggagt ggcgcgggtg ttgggagtcg ggggtcggga
    12601 gtcggagcca agcaagggac aaagcaacga ctcccggctc gcgattcagc ctgttctggg
    12661 agtggagcgt cggctgtcgg agccaggcaa ggaactaaac tccgaccccc gacccccgac
    12721 tccccacccc cgcgtcagta cctcacccgc ttcaactcca gctttgcgga agaggacgcc
    12781 gtcgagtagg gatagcggcg gatgacttct tcgaatgtct tgcgggccat gccgatgtct
    12841 ccggtcttta cgaaggtgag tccgatcttg aacatcgcgt caggggcttt gttctgctcc
    12901 gggtactcct tcgtgacgcg ttcgtacgag cgcatggcgt tggtgtagtc gccggcggca
    12961 aagtacgttt cgccgatcca gtaaagcgcg ttgtcggcca gctcgccgga cggctccgca
    13021 tcgaagatcc gttgcaacgc cgctcgcgcc tcgggcatgc gccccttgcc gaacaggatc
    13081 agcgcatcgc ggtaactgtc ggcgatcgcc gcggaacgga gcggaccact cacgggcgcc
    13141 ggtgcgtgaa ccggggcggg gatgggtcga gaaacaagag ccggtgcttc ggccacgcgc
    13201 gggaccggaa caggttccaa agcgcgaggt gccaccactg gttccgccgc gcgcgtttgc
    13261 ggttgcacga cgacgcggct ctcgccctcg gctgcctcca gtttcgcgat gcgtgaattg
    13321 agcacatcga gccgttccaa cagctcggtc atcgacacct gcaattcggc aagccgcgca
    13381 tcggtggccg gattggtcgg agcgggagcg gcgtcgggcg gcggcacgag cgacggcggc
    13441 cccgcatctt cgccggccgc gcggttggat gcgcatcctg cgagcacgag cgcgagaagg
    13501 atcgccgccg ctaattgcct catgctgtct tcatggtccc gagcttctgt ttggctttca
    13561 ctgcccacac gctctgcgga aactcggaga gaacgcgctc aaagtagagg cgtgcttctg
    13621 ctgttcgccc gaggccggag agtgcagtgc cgaggtcgaa gaacactccg tcgcgctcga
    13681 catagttcgg ataggtttca acgatgttgt tgaggcgctg gaccgcggcg ttgaagctgc
    13741 gccgtttgat gtagtaacgg gcaatgatgt gatcgtgccg cgccagacgg tcgaggacgt
    13801 cctgcatgcg cttgtccatg tccggacgca gcgggctttt cggataagtc ttcaggacgt
    13861 cgttgaactt ctcgagggct tcgcgcgtct tctgttgatc gcggtccgga cgttccatct
    13921 gcttgtacga gcacatggcg atctggaaca tggcgtaatc ggcgcggtcg ctgccgggat
    13981 aacggttgat gaagtctcgg tacttgtact gcgcttcgac gaggttcacc gagtcgccct
    14041 gctcgtagta ggtgtcggcg acgcgcagca atgcacgccg tccgagggga tcgtttggat
    14101 aggtctcgta gacgtgcgag taatacgtgc gggccttctg gtacttcttc ttcgcaaact
    14161 gatcttcgcc gagctggaac actttctcct tcggcatctt catcagagcc ggatcgaagg
    14221 tcgtgcgaag cgagcgcgtt gcgctgcttc cgccggtgtt cgcgttacgg tgaaagaagt
    14281 gccggcagcc ggcaagtgcg atcgagagga tcgcaatcag ggtcagggtc tttttcaatg
    14341 tgcctccggt agagtgatca gggactagag attagagatc agagatcagg gtgtgtttct
    14401 tcctgatctc tggtcgctga tctctactga gctctcaggt tagcgctgag ctgtctctcc
    14461 tgctccctga tctctgctcc taaactgtca cgctaacgct ctttctgatc gcttccattg
    14521 ccatcttccg gtccggctgc ccgaaaactg ccgatccggc caccaggatg gtcgcaccgg
    14581 cggcgaccac cagcggggtg gttgaaggat cgataccgcc atcgacctcg atatcgaccg
    14641 ccacccctcg ttcccggatc atctgctcaa tgagacgaat cttgcctacc acaggttcta
    14701 tgaacttttg cccgccgaag ccagggttga ccgtcatcac caggacgaag tcgcaaaact
    14761 cgatcgccgc gctcagcgat tccgcgggcg tggcggggtt gatggcgatg cctgccttcg
    14821 caccgccgtc gcggatcagc cgcagcgcac gttgaagatg cggctccgtc tcctgatgaa
    14881 tcgaaatcat gttcgcgccc gcatcgagga acgcgccgat gtactgctgc ggatgggcga
    14941 tcatgaggtg acaatcgaac ggcagcgttg tctgcgcgcg gcacgctttc acgaccgccg
    15001 gcccgatcgt caggttcgga acgaagtggc cgtccatgac gtcgaggtgg acgagatctg
    15061 cgcctccccg ctcgatgtcg cgaagctcct cgccgaggcg cgagaaatcg gccgagagaa
    15121 gcgaaggtgc gatccggatt ttcgtcgtca ttgattcgtc gtggaaggga ctgcgctcgg
    15181 aggtccaacc gcggcaggct gctcgatgat cccgccttct tcctgcctgc tgacgacgac
    15241 cgtgatggga tcgcggccgc tgacctctgc gccgcgcaac ggaaactggc ggatgataat
    15301 cccgtcggcg attcccgggt acgcctcgaa ctttacgttg gacaccttga gcccgcgccg
    15361 ctccaacgac ggacgcaccg tgtcgagcgg atagtcgatg aggtcaggca tcacgaacga
    15421 cggcggcgga accggcacgg cgacgagcaa cgagaccttg ctctgcgccg cgacgacggt
    15481 cccttttggc ggctcctcgg cgacgattcc cggcggcagc gagctatcga catatgcaag
    15541 attgtcgagc ttgaggtttt gctggccgag gcgaaggagc gccgtgcgcg ccgactgccc
    15601 atcgagatcc ggaacctgca gcaccagcgg acctgcgctg agctcgacgt ggatgtcggt
    15661 gccgcgcttc acgaggttcg tcgcgccagg agcccggttc tgccagacga tgctgcctgc
    15721 ggcgatcttg tcggaattgc gctggtgctc gggatcgacg acgagactga cgccgagctc
    15781 cgtgcatacc tcggtcgcat ccttcactga tttgcccacg aggttcggcg tcgacaccga
    15841 gcggccgcgt atgaacagcg tgaagcagag ataggtgctg acgccgaaga cgaggacgac
    15901 gagcgcagcg aacaggacgc tgtccaggaa gccgtgtttc ctcggttcac tcatcgctgt
    15961 tcgggccgct cgcttttctg atgctcagct ctgtactctg cttcgtcgta cttccggctg
    16021 ctcggcctgg cgatgtagag cggaatgaga gtctctttcc acacatcttc gacgcgtgcg
    16081 gcaaagacga agtgcaaccc gttcttcaca tcctcgggca cctcagccag atccttccgg
    16141 ttgccttccg gcagcacaac cgtcttgata ttcgagcgct gagccgccat caccttttcc
    16201 ttgactccgc caacggagag cactttaccg cgaagtgtga tctcgccggt catggccacg
    16261 tcgtgccgga ccgggcgctc ccccatgacc gaggcgatgc atgtggcgat ggcaacgccg
    16321 gcagagggcc catctttcgg ggtcgcgccg gcgggaaaat ggatgtggat gtcgtgctca
    16381 ctgaattgct tgtcgtcgat ctcgaggtcg ctggctttcg accggacata ggaatacgcg
    16441 gccgtcaccg actcgcgcat gacgtcgccg agctgaccgg tgacggtggt ccgtccgctg
    16501 ccggccatcc gcgtcgcttc gataaacatg atctcaccgc cgaactgcgt ccaggccagg
    16561 ccggtggtca cgccgatctc cggatgccgt tcggcaaatt cgtgctcgta cgatgggagt
    16621 ccgaggtacg gctcgaggtc ggcgctggta acggtgatgt gcgtctgctc ttccccgctg
    16681 gccactttgc gggcgatctt gcggagaatc gtggcgatct tgcgctccag gttgcggacg
    16741 cccgcctcgc ccgtgtaatc gcgaatcacg gtgaacagcg cgtcgtcatc gatcgtcacc
    16801 tgatcgggct cgaggccgtg gctggcaagc aactcgggaa cgagatgctt cttcgcgatc
    16861 tcgacctttt ccggctccgt gtagccggag agacggatga cctccatccg atcgcgcaac
    16921 gggctgggga tggtgtcaag catgttggcg gtgctgatga agagcgtgtg cgagaggtcg
    16981 tatggaatct cgaggtagcg gtcgacgaag gcattgttct gcttcgtatc gagcacttcg
    17041 agcaacgcgg acgcagggtc gccgcggaag tctttaccga tcttgtcgat ctcgtcgatc
    17101 atgatcagcg ggttgttgac gccgacctgg atgtagctct ggatcagttt tccgggcatg
    17161 gcaccgatgt acgtcttgcg atgcccgcgg atctccgact cgtcggtgac gccgccgacc
    17221 gccatgcgca cgaacttcct gccgagggcg tcggcgattg cggcgccgag ggaggtcttg
    17281 ccgactccgg gcggtccgac gaagcagagg atcggtcctt tgaggtcgcc cttcagcttg
    17341 agaacggcca gaaactcgag cacgcgctct ttgactttct cgaggccgtg gtgcctctca
    17401 tcgagaatct cctcggcgcg cacaaggtcg agctggtcct cggtcttcgc cgcccacggc
    17461 acctgaaaga cggtgtcgag atgccccttg atgacggcgt agtcgctcga tgacggcgag
    17521 agctgcgaga gtcggttcac ttcgcggcgg agcgattctt tgtgctcttc ggcgagcgga
    17581 agcgcctcga cgcggctgcg gtaggaatcg gcttcgcgat ccgaggagct cgaatcgcca
    17641 agctcgtcct gaatgacgcg caactgctcg cgcaggtaac cctcgcggtc gcggcgatcg
    17701 atcgaggtct ggatctggcg ctgcagctcc ttgtcgaagg ttgccttgcc gaggtcgcgc
    17761 tgaacgcccg cgacgaggag ctcgaggcgc tcgatcgggt tgactgtctc gaggaggagt
    17821 tgcttttctt cgagcggaaa gttgacgaat gacgagagga ggtcggcaaa cgtgtccggc
    17881 ccctcggtca tgttcatgcg caggatgttg agaatctcgt tcgagtactt cgggtcgctc
    17941 tcgaccagct tcccgaagag cgacatcgcc ttgttcatca ggttgtcggt cttggcgctc
    18001 ttcggaatcg gacgcggcac gacctcgcgc agcatcccct cgaagtaagg ctcgttctgg
    18061 agcatgtgca cgagctcgac gcgctcgcgg ccctgcagga agacctgata gcggtccgcg
    18121 gagagtggga gccgctggat gatcgccgcg agcactccga tctgggagag atcttcagcg
    18181 cgcgggttct ccttgtcgcc tgtcttctgg cagaacgccg cgatgaggtt ctgatcctcg
    18241 ccgagactct tcagcaggcg gacgttgcgg tcgatgccca cctgcagcga aagaacgccg
    18301 ttggggaaga gaacggacga caccagcggg atgatcggga gactcttcgg aatctcctcg
    18361 tccatccagt cgtccggatt gccggagccc ggcagttttc gattggtctg agtcatcttg
    18421 aagattgggg cgcaaggccg gtgcccggct gtgcccagcc acaccctaca cccagctccc
    18481 agttcgcgtc accctctgcc gtgcgcgggc gcggccgggg cgtcgtttcc ttgcgctaca
    18541 ctgcgcggag ctcagccacg gcacgcgaat tgtagaaaag agccgcaggt tgaacaatca
    18601 ctcaaattcg atgagcgaca cggccacggt tcagatcctc gcggatccca aagtcaagcg
    18661 ggccgatctg cattgccaca gccgctattc ggtcttcaag tacttccgcc gcgccaatac
    18721 tcgcgactgc tacaacaacc ccgaggatgt ctatcgaatc gccaaagaac gcggcatgtc
    18781 gtacgtgacg atcaccgatc acgactcgat cgacggcgcg ctctacctcc tcaacaagtt
    18841 ccccgacatg aaggacttct tcatcggcga agaggtcgag acgtactttc ccgagacgag
    18901 ccagcgcatt cacatcggcg tgtggggact gaccgagctc cagcatcgcg agatccagcg
    18961 gctgcggccc gacatccgcg atctcgtgcc ctacctgaag tcggagcggc tgatcttcgg
    19021 cgtcaaccat ctcttccaga actaccgcat gaagaatgtg gcggcgcgct acatcgccga
    19081 gctgctggag atgttcgaca tcttcgaagt gctgaacggc gcgatggcct cgtttcacaa
    19141 caagatggtc cagcagctcg tcaacagcgt gcagaaaggc ggccggcggg cctcgatgat
    19201 cggcggctcg gacgcacaca cgctgaagta cgtggccaaa gttcacacgg tatcgaaggg
    19261 cgagaccgtc ccggagtttc tcgagaacat ccgcgccggc gattgcttcg cctggggcag
    19321 cgagatgcgc ttccgcgagc tggtcgccga tatctacctg ctgacgctcg cctacaaccg
    19381 ggaagcaagc gccgatttca tgtcgcagga ctacagcgtc gccgataaga cgatgcagct
    19441 ggcaggacgc ttggcctcca tccccgccgc catctccggc atccccgccg cgatcacatc
    19501 actgaactac ctcaagcaga tcatcgtgac gaaagggatc tcgatgcggt tcgagaagct
    19561 ggtgcaggag atccggccgg gtttgaagtg aggttggagt gctctgccgc tccgagcgat
    19621 cgtttagccg ccctggaagg ttaacgttag ctagctagga cgccttgact gtctatacag
    19681 aaacttgtat acgggatggt aaagagtgtc tgacaagccg ttggtttggc tcggaagttc
    19741 gcgaatcgat gtccggacat ttccggcctt cgttcgccgg ttgaccggct ttcaactgcg
    19801 gaggactcag caaggactcg atccggacga ttggaagccg atgccgtcgg ttggcccagg
    19861 cgttcgcgag attcgaattc atacggaagg cgcataccgg gtgttttaca ttgcggcatt
    19921 caaggaagcg atctacgtgc ttcactcgtt cgagaagaag tcgagcaaga cagcagcaag
    19981 gcatctggag attggacgac agagattccg cgccttggtg caaatgaggc aacatgaaaa
    20041 gaaaaggaac cgttagggtc acaccgtcga gcggcaacgt tttccgagat cttgggttct
    20101 cacccaagga agcagagcat ctctcggtcc gcgcggattt gctcatccaa ctccagaagg
    20161 tgatcgtctc ccaaggtctc aagcaggcgg agatcgcgaa gatcctccac gtgactcagc
    20221 ctcgggtcag cgatctccta cgcggacgaa tcgatttgtt cagcaccgat acgctcctcg
    20281 acatgctggc cagccttggc gcaatcgtgc gcctggtcgt gaaggcgcct cgtaaagtgg
    20341 catgacaaag gacgatccct gcccgtggtc ggcggtcgtt acaggtactg gttaggcggc
    20401 cgggagggat ggctcgcgcg gcattacctc cagtccggca tcacgcgcga tacgatggac
    20461 ggcggcacga ttcatgggtc cgcggagtgt cagcgcaacg tcaaaaatac catcgttgga
    20521 catcatcttg atgcttgaaa cgcaatccgt atcgtaaaac gctcggcggg cattttccca
    20581 cgcttcgtcg gtaccacgga gcttgatctt gtgctgcggc ctaccgcgca gcacccttgg
    20641 gtcccgtagc tcatgtgaca cggtgggagg agatgcgagg cgtgaaatcg ctgcctcaat
    20701 cgcttcgaga cggtcgagaa cgtatttttc cgtgtcgttc tttgcgacta cgtcacgcat
    20761 gaccttcgct tcacgcgccc tgtaaatcgg gttgtcgggc gccgtctcag ctaaggccaa
    20821 attcatcgct gcctcgagtt tgggacgcac ttcatcgact ccgtgcatgt cattcgtgaa
    20881 gaagatagtg cgctcatcag caatatcgaa aggtagttcg gtaccttctt cggcaagcgt
    20941 gatgacgggg agtcggacag catgtcgcac ggctagctcg tacatcacgt tcgcattcag
    21001 cccgctaagg ttagcgacca cgagttcatc gttgagcaac cgatcgatta cttgcatggt
    21061 gattgaacct ggagcggaga tttcgtgggc agccgcgaca tcaaacccgt tcgcttgcag
    21121 cgttggacgg atgacattcg tgaccagccc atcggttgcc cggcgaactt gcgatcccgg
    21181 atcgccgata ggagtgataa caaagcagcg tttccgtttc ggctcttttg cgtccgacac
    21241 agcaacctca gaagttcaca gccagcccgc ccagataccg cctcgtcacg ccgtcggcgt
    21301 cgagcgcgtc gagcaggatg cgggagtttt cggcgcgggc gagctcgatc cctagcagca
    21361 ccttgagatc gagtggcaga tggagcgatt gtgcgaggcg caggtcgacg cgatgggtgc
    21421 ggtaggcgtc gccgccgttg ggttggggct gattgatctg acggtaggag acggcgagcg
    21481 tcgttcgcgt cggggagaat gtcgactcca cgtcaccggc gaggtagagc ttctctcccc
    21541 cgcgggacgt gacgccggga tgggctggtg aagcggtgcc cgccatcgag gagacatcga
    21601 gcaggaaacg tcgtccgagc tctttgcgga acgcgacacg aacatcgcgg cgaatgtcgc
    21661 cggcatcgat gtagaggccg tcccagaaat gttcgagtcc gtccgtgaag acgatgcgga
    21721 gcggcgtatc gaccgcgctg acgctgccga cgacgctgaa gtgctcgcgc gaattgtcgc
    21781 ccgagacgat gccgacagag taggcgtacc gcggcagttg tcccgagtca tcggtccaga
    21841 ccacgacgct cggtaaggtg ttttgatgtt ctgagtcgac gactttgtac atcccggaga
    21901 cgaccagcgc cgtctccttc ccgactttcc actcggcgcc gctgcgcggt gcccattcgg
    21961 ttccgtagag gccgacgcgg ctggacatcc catagtcgac cgtgaacgcc ggcaccagtt
    22021 cgaacttcgc attcgcggtg acgtcggccg ttcgcaaggt tggcgcagct acggctcccg
    22081 cggtcgtgcg cgaggtctcc tgcgtcacgc gcagcgaaac gccgaggtcg ttgcgcggcg
    22141 tttgaaagac agtggtgtcg cccctgatct cgatgagctc ggaaccgggg gaggcaacga
    22201 aaaggttctg ctgcgcgagg taacgaacct gtacgtgcga gtcgccgtgc gcccactcga
    22261 agtcatgcga acggatatcc gcctgcgatt cagaaggaat ctcgtcacgg taacgccacc
    22321 acgagcgggt cgaggtcagc cggtacgaat cggtgggcga cgatcggatc tcgatggctg
    22381 ccgcgctgga tgcggcgacc ggagtgccaa aggattcgtt gtcggtcggg tcttcgacac
    22441 gatgaaggtt gccgtggaaa ccgatctgcc agttgtcgcc gacgcggctt tgcagtccga
    22501 gcgcggtctg ggcgaacgcc ggaccggacg gcgaggtcgt cgtgcctgcg gacatgccgg
    22561 tcatcgacac catctcgccc ttgaatcggg gaacttcgta agccaacgcg ccaggtccga
    22621 tcggatcggg cggcggcgcc atcaccatat cgatctggcg gaggacatca ggcggcagcg
    22681 agccccgaat ctcccacatc tcctgattcg tgcccttcga ttgcttctcc gtctcgagcc
    22741 gcagcttcaa tcgatgctct ttcttcgtcg gtacgatggt ctcgatcgcc ggcgtgaagc
    22801 cgtacttcat ggcgatgatg cggtagacgc cggcaggaag ttgcggcagc gaaaacgagc
    22861 cgtcgctact cgtgaacgtc tggatcgcgg cgaggttgtt gaggttgagg gcgatgacga
    22921 gagcattgcc gacaggccgc gcggagttgg tcacgctgcc gagaacctgc agcaccgccg
    22981 gaagcgggac ctccgattgc gcaggaacag cgaagacgat ggcaagtgaa gcaatcgcga
    23041 tcgcacgcag tcggcgcata ggccctcctt ctcaacaagc gagtgtatgg agtcgcttgt
    23101 cccgagtcaa atgacggaac gtgaggtttt ggtgaggact tacagattgt tcacgcgaag
    23161 tcgcgaagcc gcgaagtgag aaacgtgtct cgcccctccg cggcttcgcg gcttcgcgtg
    23221 atatatgaaa cgtgatgcgc cgcaccaaaa tcatcgccac gctcgggccc gcttgcggct
    23281 ccgaagacgt cattcttcgc ttgttaactt ccggcgtcga tgtcttccgg atgaacttct
    23341 ctcacggaac gcccaccgac cggctgcgcc tcatcgagat tattcgtcgt gtggctaccg
    23401 accacggcaa gtacatcccc atcctcggcg acattcaggg acccaaacta aggatcggcg
    23461 aggttgacgg ggtcatccac ctgcagccgg gtcagatgtt caccatcacg accgaggccg
    23521 tggcaggaaa cggctccatc gtctccacgc ccttcactcc cctgccgcgc gaggtgcaaa
    23581 tcggccagcg gatcctcatc aatgacggcc tcgtcgagct cgtcgttgct gcggtcgacg
    23641 ccacaaaggt gctgacgcgc gtcgttcacg gcggaccgat ctcgtcgaag aaagggatga
    23701 actttcccga caccgagctg accattccgg cgattactga gaaggaccgc gaggatgtcg
    23761 ccttcgctgt cgccaaccag ctcgactaca tcgctgcgtc gttcatcagg cggcggtccg
    23821 acatcgtgga gcttcgctcg ctgctccgcg acctcggcgg cgacgagatc aacatcatcg
    23881 ccaagctcga aaagtcgcag gccatcgatg cgctcgaaga catcctcgac gtcagcgatg
    23941 gtgtgatggt cgcgcgcggc gacctcggcg tcgagctgcc acccgaggat gtgccgatcg
    24001 tgcagaaacg cgtgctgcag aaggcggcgg tctgggggcg ctttgccatt accgccacgc
    24061 agatgctgga gtcgatgacc tcgaactcgc ggcctacgcg cgccgaggcg tcggatgtgg
    24121 ccaacgcgat cttcgatgga tcggatgccg tcatgctatc cgcggagacc gcgtccggcg
    24181 cctatccggt ggaagccgtg cagatgatgg cgcgcatcat catggcggcg gagcagaatc
    24241 gccagtttca gatggccggc ggacgcgatc ccttccgcgt ttcgacggag accgacgagt
    24301 tcacagacgc cctggccggc gccgccaact acgctgccca gcagctcgac gccaagttca
    24361 tcatcgtctt cacgcagacc ggctttgccg cgcggctgat gtcgaagttc cggcccaaac
    24421 agccgatcat cgctctgacc ccgtcgacgt gggtcgcgcg ccgcatgaac gtgctgtggg
    24481 gcgtgcagcc attcgtgctg cgcgaggttg gtgagtatca cgagcagatc gtcgagcggg
    24541 tcgatgagtt tctcatcagc cgcgacatcg tcgtcccggg cgaccggctg gtgattctga
    24601 tggggtcacc ggtctaccag cgggcgaaaa cgaacctgct gcgggtgcat cgggtgaggg
    24661 agtcgtagga catcgggggt cggacgtggg tgtcgcttgt cgggggtcgg gggtcggaga
    24721 agagccagcc cctgccgcga cgtcatctga agacgacgga agccttatga tttcggtaga
    24781 cgccttacct tcttgaagta cgtcccgaac ctgtttggta gggttttgac cttcgtgaaa
    24841 tacgtcccgg acctgtttgg tagggttttg acattcgtga aatacgtccc ggacctgttt
    24901 ggtagggtct tgaccttcgt gaaatacgtt caggacctgc ttcggtaggg ctttgacctt
    24961 ctggaaatag gtcccggacc aatttcggta gggtttttgg cttcttgaaa taggtcccgg
    25021 accaatttca gtaaggtttt gggcttcttg aaataggtcc cggaccaatt tcagtagggc
    25081 tttgaccttc ttgaaatagg tctcggacca atttcggtag ggttttgacc ctcttgaaat
    25141 aggtctcgga ccaaataccg gtagggtttt gacctttttc aaattggccc ggccgtcttg
    25201 cacggatcta actccgaccc ccgacgcccg acaaacgact cccgcgactt cacaccgtcg
    25261 ccgcaagcgc cggttgcagc gatcttccca ccacgcgcgc gatggcatcg cactcccgca
    25321 cgatcaccgc aaacgcctcg ggcaggatcg cctgcggtcc atcggacagc gcttcctccg
    25381 gcttgtggtg gacctcgatc aacacgccgt ccgcgccggc agcgatcgac gcgcgcgaca
    25441 tcggcgccac tttgttgcgc ttgccggttc cgtggctggg gtcgacgagc accggcagat
    25501 ggctgatgcg cttgatggcc gggatgatgg agagatcgag cgtgttgcgg gtgtgatcgg
    25561 caaacgtacg cacaccgcgc tcgcacagga tcacgttgta gttcccctcc gccatgatgt
    25621 actcggccga gagaagcagc tcctccagcg tcgccgacat cccgcgcttc agaagaatcg
    25681 gcttgcgcgc gcgtccggcc gctttgagca gcgagaagtt ctgcatgttg cgggcgccga
    25741 tctggatgca gtcggcgtac tggtcgacca gcgcgagcgc ctcgttgtcg atggcctcgg
    25801 tgacgatggg aaggtcgaac tcctcgcgaa tcctcgccag aatctccagc cccggttcgc
    25861 ccaatccctg gaacgagtag ggcgacgtgc gtggcttgta cgcgccgccg cgaaacaact
    25921 gccctcccgc gtttcgaatc ccctcggcga tcgcacgcga ctgatcgagc gactccactg
    25981 cacacggtcc gccgacgacg atgatgccat tgccgccgac atcgacgcct ccgacattga
    26041 tgacgctgtc atccggccgg gcctcgcggc tgacgagctt gtaagcgtgc gagacgcgga
    26101 tgacttcctt gactccggga tagttctcga gaacctcggg gtccgcggtc ccgggttttc
    26161 cggtgatacc gattgctgtc cggttcgctc cggggatggc atgggccttc agacccatct
    26221 tctcgatatg gcggacaacc gcttcaattt gatcgggccg ggctccggct tccatgacga
    26281 cgagcattgg tggccagtat aatcgacggt cctgaagcgc cattccgagg agccccgcgt
    26341 gaaaaagaga atgatcgttg ccgttcttgc tgccgtcgtt gccatccccg ccttcgctgc
    26401 cagctactgg gtcgtgctca aggatgggac gcgctacgag gcgaagtcga aatggacggt
    26461 cgtcaacggc aaggctgtcc tgaacctcgt caacggcacg gtgctcaacc tcgatccaaa
    26521 cgtcatcaac gtcgccaagt ccgaggagat gacccggctg ggcggcggag acctcttcgg
    26581 tgtcgagcag attcctgtca cgtcgacgac gaaaacatcc cagctcggcg cgaccatcaa
    26641 gctgcacaaa cttccgccag cgcaagctcc cggtgcggcg gcaccagtcg aggcgaccat
    26701 ccccgtcgct ccaggcgcac tcctcggtac ggacgtcctc ggcaagttcg agcgcgccta
    26761 cgagaacgtc ggaatcttcg agcacaagct catcgccacc ggtccccact cccttcgcgc
    26821 cgacctcacg gcggacaccg aggacaaagt cttcaacacg ctctcggcaa ccgcgttcct
    26881 gatggttcgc aatgccggga tcacgggagt gcagatcgat tacgccgaca tcttcatgaa
    26941 aacgacgact ggcgccgctg caggccgttt tcatctcacc cacgacgaag cgcaggcaat
    27001 cgagtcgaag acgatcacgc cgcagtcgta ctttgtgctg aaggtgctct tctgagagac
    27061 agagacaagg gattagagat tagagatcag agatcaggtg gaatcattaa gcctctctat
    27121 ctctgatctc tgctcttctg atctctcctt ctcaacagag acaagggatt agagatcaga
    27181 gatcacgtgg aatcagcaga caagtctctt atctctgatc tctgcctctt ctgatctctg
    27241 cttctcaccg cagcacgtcg accgtcgccg cgccgccata gacggtgatg tagcgaaagt
    27301 tgcggatctc ctcatcgagc aggcttcgtt ctcccgccgc gaacacgaac tgctccaccg
    27361 gctcatcgac cgtgaatagc tgcgcgcgct cgtaggcgaa gcggacggtg acgttgcgcg
    27421 gtccgttctc gacctttcgg atcgtgagcc tcactcgccc gctcgtcacc gtcccgaagt
    27481 cgtacgccgt cgccgccgtc tttcgcgaaa tcatcaccgc ggtaccgccg atgaccgcaa
    27541 tctccggaag cgcagtcttg aagtcgaagg tttgctgcgc gccaatgact tgctttgtcg
    27601 gagcaaagaa tgccgcgtcc ttctgcgtca gatagttcca gcgccgtgcc ggtggacgtc
    27661 ccagaagttg cggacgcctg atctcgccgg ccggaggatc gcggacgagc agatcgtcgt
    27721 gccaccgctg cacgatgtgc cagtcgctcc cggtgacgat gaagttcgcg aactcgcggg
    27781 tcagatcaac cgcagcgatg agtcctccca cgccgttcgc gctgcgcacg gccacgacca
    27841 tgcggttccc atgatcgtgc gccagattgc tgacgtcgta tgtgtcgagc gcctgattac
    27901 catcgccaac ccggcgtccg ccgaccgcca cgccgttgaa atacagcgtg tactccggat
    27961 caccaagcat cttgatccgc gtgaagtttc gattcggcgg aagatcgaaa tcgtacgtcg
    28021 caaagaacgc caccggatca ccctcagcca tccgatgcac cggccagatc cactgagccc
    28081 gtccggtatt gtcgaagaac ttgtgcgagt agagcagatc gagccggctg tacgcagcaa
    28141 cgccgatgaa cacgaccgcc gcgaggatga gcaagcggcg gatgaggatt cgttctcggg
    28201 gggcggggag tggggagtgg ggag
//
LOCUS       DATLVW010000833         3241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_117879, whole genome shotgun sequence.
ACCESSION   DATLVW010000833 DATLVW010000000
VERSION     DATLVW010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117879"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VNN25_24450"
     CDS             <1..1030
                     /locus_tag="VNN25_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94750.1"
                     /translation="FPRDKLCGEFLSYDALPIIDALGLRDELDAHAAPEISHCRIVGR
                     HRNYEFDFPHPARGVSRMLLDDALFRRAVADGAHPVTGTASEVTRDSVTFDGGAVRAR
                     VVAGAWGRWGRFDQQLGRAFVRDRSHRNFGFKRHYRGGTSDGTIELYSFKRGYLGVSE
                     VEGGITNICGLVHADRLQGLKGRWDAFIEAIRAEERALEEMYARHTPAQEGFLSSEPV
                     IFRSRSAVEEGIFMIGDASGVIDPLTGNGMAMALQSALVAAPFIAAALENGDRRHSED
                     AYRARHAQLFGRRIGWSRRVAFLLSRPALLDAVLRLHVPSAGPFFLRKTRADRDAIAS
                     MVDVELRT"
     gene            complement(1103..1765)
                     /locus_tag="VNN25_24455"
     CDS             complement(1103..1765)
                     /locus_tag="VNN25_24455"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXH94751.1"
                     /translation="MTLARESEFLLPLFPLPNLVFFPETHLPLHVFEPRYRQLIADVS
                     ARDHRFGIVLLQPGWENDYFGSPPVYTCGTMVRIEEAMPLDDGRYNIVVSGETRFHIV
                     NEVSREPYRTARVIARPQNEDDPAAAYAQRTWLADLSQQYLHFLPEGGSVPVMQTVGL
                     EALTNSIVMALNIDVVEKQRLLEIDDVTARAEDVGSELQHRIESMQFLAPFRQGGDPN
                     RN"
     gene            complement(1762..2274)
                     /locus_tag="VNN25_24460"
     CDS             complement(1762..2274)
                     /locus_tag="VNN25_24460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94752.1"
                     /translation="MRSTVASMLALNVALITGALVLAGCQKKQETAAPGRRVITTNTP
                     QNESGKRASVIDRVGTAVIEKMLVGIQLGPDGTVSEQQTVVPAGDPVYVTLRLRDSPV
                     GLRTSAVWYDAHDQQIAREQKDMNGTKVATFALSQKLTPGKYHVRGYWGGNIADDREF
                     VVVPKSKRSK"
     gene            complement(2294..2986)
                     /locus_tag="VNN25_24465"
     CDS             complement(2294..2986)
                     /locus_tag="VNN25_24465"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXH94753.1"
                     /translation="MAPLTLHHDAAPLHAQYGSAEPFPHIVLDGLFDDEALDAVLREF
                     PDRKAMKWREFDSPTEKKLGYYHESSSISKTVRDFLNDMNSFEMLLWLEALTGIEGLI
                     PDPYFGGGGLHQIEPGGFLKVHADFNVHPKLKLDRRLNLLVYLNKDWRDEYGGHLELW
                     DRDGKSCRKKILPLFNRTVVFSTSDTSFHGHPHPLTPPAGGSRKSVSLYYYTAGRPEE
                     ERSAAHDTLFIP"
     gene            complement(2974..>3241)
                     /locus_tag="VNN25_24470"
     CDS             complement(2974..>3241)
                     /locus_tag="VNN25_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94754.1"
                     /translation="SSAPLSIEFHHRGETLLWQFGPYRDGKYRVLRANGQQAFDVPAS
                     DAFRLGDLPGISLRVEYQSPAGWVTYSPDFALDFVHHPDVTWRR"
BASE COUNT          575 a         1042 c         1026 g          598 t
ORIGIN      
        1 ctttccgcgc gacaaactgt gcggcgagtt cctctcctac gacgccctcc ccatcattga
       61 cgccctcggc ctccgcgacg agctcgacgc ccacgcagcg ccggagatct cccactgccg
      121 gatcgtcggg cgacatcgca actacgaatt cgactttcct catcctgctc gcggcgtatc
      181 gcgcatgctg ctcgatgacg cgctcttccg ccgcgccgtc gcagacggag cgcacccggt
      241 caccggcacg gcctcggaag tgacacgcga cagcgtgacc ttcgatggcg gcgccgttcg
      301 tgcgcgggtc gtggccggcg cgtggggacg ctggggccgt ttcgatcagc agctcgggcg
      361 cgcctttgta cgcgatcgct cccatcggaa cttcgggttc aaacggcact atcgaggtgg
      421 caccagcgac ggaacgatcg agctctattc gttcaaacgc ggctacctcg gcgtcagcga
      481 agtcgaaggc ggcatcacga acatctgcgg tctcgtgcac gcggaccgct tgcagggtct
      541 caagggacgg tgggatgcat tcatcgaagc cattcgtgcc gaggagcggg cgctcgagga
      601 gatgtacgcg cggcacacac cggcgcagga aggatttctc tcatcggagc cggtcatctt
      661 ccggtcgcga tccgcggtcg aggaagggat cttcatgatc ggcgatgcct cgggcgtcat
      721 cgatccgctg accggcaacg gcatggccat ggcgctgcag tcggcactcg ttgccgcgcc
      781 gttcatcgcc gcggcgctcg agaacggcga ccgtagacac agcgaggacg cgtatcgcgc
      841 ccgtcacgcg cagctcttcg gccgccgtat cggatggagc cggcgcgtgg cgtttctact
      901 gtcccgtccc gccttacttg acgccgtcct tcgcctgcac gttcccagcg ccggcccatt
      961 cttcctgcgc aaaacgagag cggaccggga tgcgattgct tcgatggtgg atgtggagtt
     1021 gaggacgtag cacgcgaaga cgcgaaggac agcaacaaaa actcttcgcg tcttcgcgtc
     1081 ttcgcgtgag cgacccccgc cctcagttcc tgttcggatc cccaccctga cggaacggcg
     1141 ctaggaactg catgctctcg atcctgtgct gcaactcgga gccgacatcc tcggcccggg
     1201 ccgtgacatc gtcgatctcc agcaggcgct gcttctccac gacatcgatg ttcagcgcca
     1261 tgacgatcga gttcgtcagc gcttccaggc cgacggtctg catcaccggc acgcttcccc
     1321 cctcgggcag aaagtgcagg tactgctgcg acagatccgc gagccacgtg cgctgcgcgt
     1381 acgccgccgc cgggtcatcc tcgttctgcg gccgtgcgat gacgcgcgcg gtccggtacg
     1441 gctcgcgcga cacttcgttc acaatgtgga agcgcgtctc gccgctgacg acaatgttgt
     1501 agcggccgtc gtcgagcggc atcgcctctt cgatccgcac catcgtgccg caggtgtaga
     1561 cgggcggcga cccgaagtag tcgttctccc aacccggctg caggagcacg atgccgaaac
     1621 ggtgatcgcg cgcgctcacg tcggcgatga gctggcgata acgcggctcg aagacgtgaa
     1681 gcggcagatg cgtctccgga aagaagacga ggttcggcag cggaaacagc ggcaggagaa
     1741 actcgctctc acgcgcaagt gtcatttcga tcgcttcgat ttcggcacga caacgaactc
     1801 ccggtcgtcg gcgatgttgc caccccagta gccccgcaca tggtacttgc ccggcgtcag
     1861 tttctgactg agcgcgaacg tggccacctt cgtcccgttc atgtccttct gctcccgggc
     1921 gatctgctgg tcgtgcgcgt cgtaccagac cgcactcgtg cgcaggccga ccggcgagtc
     1981 gcgcaaccgc aaggtcacgt agaccggatc gcccgcgggg acgacggtct gctgctcact
     2041 cacggttcca tccgggccga gttgaatacc gacgagcatc ttttcgatga cggcagttcc
     2101 gacgcggtcg atgacgctgg cgcgcttgcc ggattcgttt tgcggagtgt tcgtcgtaat
     2161 cacgcgccgt ccgggagccg ccgtctcctg cttcttctgg catccggcaa ggacgagcgc
     2221 gcccgtaatg agggcaacgt tgagggcgag catggaggca acggtacttc gcatcggcaa
     2281 cgattctacg gcatcacgga atgaagagtg tgtcgtgcgc cgctgaccgc tcctcctcgg
     2341 ggcgtccggc cgtgtagtag tagagcgaga cggacttgcg cgatcccccg gccggcggcg
     2401 tgagcggatg gggatgtccg tggaacgacg tgtcgctcgt cgagaagacc acggtgcgat
     2461 tgaagagggg aaggatcttc ttccggcagc tcttcccatc gcggtcccac aactccagat
     2521 ggccgccgta ctcgtcgcgc caatccttat tcaggtagac gagcaggttc agccgccggt
     2581 ccagcttcag cttcggatgg acgttgaaat cggcgtgcac cttgaggaaa cctccaggct
     2641 cgatctgatg cagaccgcct ccgccgaagt aaggatcggg gatcagcccc tcgatgccgg
     2701 tgagcgcttc cagccagagc agcatctcga acgaattcat gtcgttcagg aaatcgcgca
     2761 ccgtcttcga gatgctgctg ctctcgtggt agtagccgag cttcttttcc gtcgggctat
     2821 cgaactcgcg ccacttcatc gccttccggt cgggaaactc acgcagaacc gcgtcgagag
     2881 cctcatcgtc gaaaagaccg tcgagcacga tgtgagggaa cggctcggcg cttccgtatt
     2941 gcgcgtgcag cggggcagca tcgtgatgaa gagtcagcgg cgccatgtca cgtcgggatg
     3001 atgcacgaag tcgagtgcga agtcaggcga ataggtgacc cagccagcgg gcgactgata
     3061 ttcgacgcgc agtgagatgc cgggcaggtc gccgagccgg aaggcgtcgc tcgccggaac
     3121 atcgaaggct tgttgtccat tggcgcgcaa cacccggtac tttccgtcgc gatagggacc
     3181 gaactgccag agcagcgtct cgccccgatg atggaactcg attgagagag gcgcactgga
     3241 g
//
LOCUS       DATLVW010000834         4357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_323728, whole genome shotgun sequence.
ACCESSION   DATLVW010000834 DATLVW010000000
VERSION     DATLVW010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323728"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..744)
                     /locus_tag="VNN25_24475"
     CDS             complement(52..744)
                     /locus_tag="VNN25_24475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXH94755.1"
                     /translation="MKLSDVRALVTGGTSGIGFEIARQLKEKGGDVVICGRDDAKLLE
                     AADKIGAHGVRADVGVEREAVDLVRQTIALLPGYNVLINNAGFGSFAPLLDTDLPAMQ
                     QMFATNVFGAMVVARESARHFVARKRGNIINMSSSAGVRGFAGGTAYAASKFAVSGMT
                     ECWRAELRKHDIRVMQINPSEVQTEFHRGGRALSDRKLRAEEIAHAALSMLEMDDRGF
                     ITELSVWATNPD"
     gene            complement(1242..2840)
                     /locus_tag="VNN25_24480"
     CDS             complement(1242..2840)
                     /locus_tag="VNN25_24480"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXH94756.1"
                     /translation="MPDFETIAAPVAAEANRAERLALRVVQVGAIAVVIVVSTLHIFE
                     LDRFFVPKELAFHLTAVVAGLLLIRAMGRLAITRVEVFLACYVLLSAISALMATNRWL
                     ALRALAISASSVVLFRVARTLRNAGLARAVIHGLGLAVMLVAVMSLLQTYGVRTDFFA
                     ENRSPGGTLGNRNFVAHVAAFGLPLLLFSAMGARSRRAYVWRAAGAALVVVALVLTRS
                     RAAWLAFAVTAVVVLAAMLFSKALRRDARTWKRLAGIGLIAAAGIGAALVLPNTLHWN
                     DDNPYLESLRRVTDAQEGSGHGRLVQYGRSLLLAARHPLFGVGPGNWPVTYPAHAAKN
                     DPSLDQGDTGMTSNPWPSSDWMAWLTERGPAAFLLLLFAVLKVARDGLRQLRRARDAE
                     EAIAAVVLLATLAGACVAGAFDAVLLLAVPAFLVWAAIGSLWIPEESPSRPAWQYAFV
                     AMILVSAIGVYRSAKQLMAMSLFSAGGRASITRAAEIDPGNYRIQMRLARMGGRAHCE
                     HARAAHDLFPTARAAAQVARGCGK"
     gene            complement(2883..3032)
                     /locus_tag="VNN25_24485"
     CDS             complement(2883..3032)
                     /locus_tag="VNN25_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94757.1"
                     /translation="MIRKLKTGEYRLYSRKKNPSTGKRRNLGTFKTRAAAETHEREVQ
                     YFKRH"
     gene            3214..4122
                     /locus_tag="VNN25_24490"
     CDS             3214..4122
                     /locus_tag="VNN25_24490"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH94758.1"
                     /translation="MTRRLFAVLVLFLFAAAGQAGDLVSGVRYKLSAGDLASGIAAVE
                     DYKLATGVDAEYLNAIGWLARGAEMEGKSEVAKEYVAELHREIPVEKADLLTPFGAAI
                     EVEGRLIAAQNGRGAAIRYFTGELEHATAPSLRSRINKNINLLSLEGHQPPALETAKF
                     IGAAPVSLEALRGKPVLLYFFAQGCGDCRAQAPSLTRVWKKYQSKGLSLITATRLYGS
                     VGENNKATPAEEQAQIEKVWGELYAGLDGVPAIIDTETMVRYGVSATPTFVLIDRKGL
                     VRMYAPTRISEAELSRRIDAVLAEAP"
     gene            complement(4133..>4357)
                     /locus_tag="VNN25_24495"
     CDS             complement(4133..>4357)
                     /locus_tag="VNN25_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94759.1"
                     /translation="CSARSVPRKKKEKHMARGGGNAPTNITWIICLILYIVALAGHFG
                     HIQAITPVIALWSWIIGLGLLLVACRVRGL"
BASE COUNT          855 a         1406 c         1371 g          725 t
ORIGIN      
        1 aaggctctcc tctgtgacct ctgtgtctct gtggttcagc ctttctcgaa gctaatccgg
       61 attcgtcgcc caaaccgaca gctcggtgat gaagccgcgg tcgtccatct ccagcattga
      121 cagcgcagcg tgcgcgatct cctcggcgcg gagtttccgg tcactcagcg cacggcctcc
      181 gcgatgaaac tccgtctgca cttcgctggg attgatctgc atcacgcgga tgtcgtgctt
      241 tcgcagctcc gcgcgccagc actcggtcat tccggagacg gcgaacttgc tcgccgcgta
      301 ggcggtccca cccgcgaagc cgcgcactcc ggcagacgac gacatgttga tgatgttgcc
      361 gcgcttcctg gcgacgaagt ggcgcgccga ctcgcgcgcg acgaccatcg ctccgaacac
      421 gttcgtggcg aacatctgct gcatcgccgg cagatcggtg tcgagaagcg gcgcgaacga
      481 gccgaagccg gcgttgttga tcaggacgtt gtacccgggc agcagcgcga tggtctgccg
      541 cacaaggtcg accgcctcac gctccacgcc cacatcggcg cggacgccgt gtgcgccgat
      601 cttgtccgcc gcttcgagca gcttcgcgtc atcgcgccca cagataacga catcgccacc
      661 tttctctttc aactgccggg cgatctcgaa tccaattcca gaagtgccac cagtgacaag
      721 cgcgcgaacg tcggaaagtt tcatgcgggc attctagtgt cgggagtcgc gtgtcggggg
      781 tcgggggtcg ggggtcggag aagcagatcg gccttttctc gtcgactccc gacgcccgaa
      841 acgcgacttc cgacgaaaag gtgcctgttt tccgtgcaaa aatggaatca agtagaaaac
      901 catttcacac taatgtgaaa cggatcacat gagtgtgaaa ccagattcca tgaacggtaa
      961 atcatttcac actaatgtga aaccagatca catgagtgtg aaaccagaaa ccgttaatgc
     1021 gaaatcggaa cgcgttaacg ggcaatcgga atgagtgaga cgcgaaattt gacccgccaa
     1081 cccgttcggg gggtcggggg tcgggggtgg ttatcggaga agaagaccgg cgtttctttt
     1141 ctccggctcc ccgactccca acacgcggtt cccgcaaccc ggatgtgaga aggaggtcgg
     1201 cgtttctttc tccgactccc gacaaacgac tcccgcgaac atcacttccc gcacccgcgc
     1261 gctacctgcg cggccgcacg cgccgtcggg aaaaggtcgt gcgcggcgcg ggcgtgctcg
     1321 cagtgcgcgc ggccgcccat gcgcgcaagg cgcatctgga tgcggtagtt acccgggtcg
     1381 atctccgcgg cgcgggtgat cgaagcacgg ccacccgctg agaagagact catcgccatg
     1441 agttgcttcg cgctgcggta cacgccgatg gccgacacga ggatcatcgc tacgaacgcg
     1501 tactgccaag ccggccgtga tggagattcc tccgggatcc acaacgaacc gatggccgcc
     1561 cacaccagaa acgccggcac ggccagcagg agtacggcat cgaacgcacc cgccacgcac
     1621 gcaccggcga gcgtcgccag caacacgacc gcggcgatcg cctcctcggc atcgcgcgca
     1681 cgccgcaact ggcgtaatcc atcccgcgcg accttgagaa ccgcgaagag tagcagcagg
     1741 aaggcggcag gaccgcgctc ggtgagccag gccatccaat cgctgctcgg ccacggattc
     1801 gacgtcatcc ccgtgtcgcc ctggtcgagc gatggatcat tcttcgccgc atgcgcagga
     1861 taagtaaccg gccagttgcc aggaccgaca ccgaacaacg gatggcgcgc cgcaaggagc
     1921 agcgaccgcc cgtattgcac gaggcgcccg tgcccgctcc cctcctgggc atcggtgacc
     1981 cggcgcaacg actcgagata cggattgtcg tcgttccagt gcagcgtgtt cggcagcacc
     2041 agcgccgcgc cgattcctgc tgcggcgatg agcccgatgc ccgccagccg tttccacgtc
     2101 cgcgcatcgc gtcgcagcgc tttcgaaaag agcatcgccg caagcaccac gactgcggtg
     2161 accgcgaacg ccagccacgc ggcgcgcgaa cgcgtcaaca cgagcgcgac gaccacgagc
     2221 gcggcgccgg cagcgcgcca cacatacgcc cgcctgctgc gcgctcccat cgcgctgaaa
     2281 aggagcagcg gcaatccgaa cgccgccacg tgcgccacaa agttgcggtt gccgagggtg
     2341 ccgccggggg agcggttctc agcgaagaaa tcggtccgca cgccgtatgt ctgcagcagc
     2401 gacatcaccg ccacgagcat gaccgcgagt ccgagtccgt gaatgaccgc acgagccagg
     2461 ccggcattcc gcagcgttcg cgccacgcga aagagcacaa cgctcgaggc gctgatcgcc
     2521 agtgcgcgca acgccagcca tcgattcgtc gccatcaacg ccgagatcgc gctgagaaga
     2581 acgtagcagg ccagaaacac ctcgacgcgt gtgatggcga ggcgacccat cgcgcgaatg
     2641 aggaggagtc ccgccacgac ggcggtcaga tggaaggcca gctccttcgg gacgaagaag
     2701 cggtcgagct cgaagatgtg cagggtgctg acgacgatca cgacggcgat cgcacctacc
     2761 tgcacgacgc gcagagccag tctctcggca cggttagcct cggcggcgac aggagcagca
     2821 atcgtttcaa agtccggcat cggcaacgag cgctacccta ccgcgaaacg gcgcggcagc
     2881 ggtcagtgtc gcttgaaata ctggacttcc cgctcgtgcg tctcggctgc cgcgcgcgtc
     2941 ttgaacgtgc cgaggttgcg gcgcttgccg gtgctggggt tctttttgcg cgaatagagg
     3001 cggtattcgc ctgttttgag cttgcgaatc atggcggttg ggagtgcaaa tggatcgcca
     3061 gttttgggcg atggattggg gtcgccgccg ggccggcggc ggttcagccg gcgggccgcc
     3121 ggcgctacgt ttttcgcacg gcgctccact cgcgagaaac ctttgccacc cttcccgcgt
     3181 ctcaaatgaa gacaaatcga aatggaggct tttatgaccc ggagactttt cgcagttctt
     3241 gtcctcttcc tgttcgccgc agccggccag gctggtgatc tggtcagcgg tgttcgttac
     3301 aaactttcgg ccggcgacct ggccagcggc atcgctgctg tcgaggatta caagctggca
     3361 accggcgtcg atgccgagta cctcaacgcg atcgggtggc tggcccgcgg cgcggagatg
     3421 gagggaaaga gcgaggtggc gaaggaatac gtcgccgagc tgcaccgcga gatccccgtc
     3481 gagaaggcgg accttctcac tccctttgga gccgccatcg aggtcgaggg tcgcctgatc
     3541 gccgcgcaga acgggcgagg ggcggcgatc cggtacttca ccggagaact ggagcacgca
     3601 accgctccct ccctccggtc gcgaatcaac aagaacatca atctcctgtc gctcgaaggg
     3661 caccagcctc ccgcgctcga gactgcaaag ttcatcggag cggcgcccgt atcgctcgag
     3721 gcactccgcg gaaaaccggt gctcctctac tttttcgcgc agggatgcgg cgactgccgg
     3781 gcgcaggcgc cgtcgctgac gcgcgtctgg aaaaagtatc aatcgaaagg gctgtcgttg
     3841 atcacggcca cgcgtctcta cggcagcgtc ggcgagaaca acaaggcaac gcccgccgag
     3901 gagcaggcgc agatcgaaaa ggtgtggggc gagctttacg cgggactcga cggcgtgccg
     3961 gccatcatcg ataccgagac gatggtgcgc tacggcgtct cggccacgcc gacgttcgtc
     4021 ctcatcgatc ggaaaggcct cgtgcgcatg tacgcgccaa cgaggatttc cgaagcggag
     4081 ttgtcgcgcc ggatcgacgc ggtgctcgcg gaagcgccgt gaaccgcagg cgctacaacc
     4141 cgcggacgcg gcaggctacg aggagcaggc cgagtccgat gatccacgac cagagcgcga
     4201 tcactggagt gatggcctgg atgtggccaa aatgcccggc cagcgccacg atgtagagaa
     4261 tgagacagat gatccaggtg atgttcgtcg gtgcattgcc gccgccacgt gccatgtgct
     4321 tctccttctt tttccgggga acactcctgg cactgca
//
LOCUS       DATLVW010000835         6679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_204768, whole genome shotgun sequence.
ACCESSION   DATLVW010000835 DATLVW010000000
VERSION     DATLVW010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204768"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VNN25_24500"
     CDS             complement(<1..901)
                     /locus_tag="VNN25_24500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH94760.1"
                     /translation="MLDVSVHNLSFAYAGFALRDVTLTFPRSTHTAVVGPAGCGASTL
                     LQIMAGAVRPDSGEVRIGSRVVNDLKAGRRPLLHAVSSIDAPRRWSVRHLLIAAARQR
                     TLDREDRQHEVELATSKWRLGDLLDRRLDSLSSTEVVRAHLARIEILKPAILLGDRML
                     ERLNLAESVSIVDEWYRTLRVLGTTVISAPSSLIELGASDRVVVLDNGRVVQQGVPSE
                     IYGDPRSEAAAMATGDVNAIPVTVRGNVVESLIGSWDLATPPFQGSGIALARPADFTL
                     AGPNEESDLIFGVEEASFYDGAWH"
     gene            1163..1576
                     /locus_tag="VNN25_24505"
     CDS             1163..1576
                     /locus_tag="VNN25_24505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH94761.1"
                     /translation="MSIQARRTPFKLRKLLVIDDDAKLLASYRDLLLPYGFQVLTASD
                     GRDAIPLVEQNPDILLVILDLAMPRMDGREWLRWFRGCRKESPVIIISAYKLEASDED
                     LKPAVILEKPFHVAELLDLVGLFCGLGTPMANEVS"
     gene            1584..2630
                     /locus_tag="VNN25_24510"
     CDS             1584..2630
                     /locus_tag="VNN25_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXH94762.1"
                     /translation="MARVGIIGTGWGARVQAPAFREAGLEVVAIAGHNADRTRRAAGE
                     LGLEAFDDWRALIASNIDLVTIVTPPSEHLSMASTALDAGKHVLSEKPMALHVAEAEQ
                     LVAAARRHPDQLALIDHELRFLPSFRAARERIAELGGVRYAEVRYASPGRGDRSRGWN
                     WWSDAKQGGGIWGAVGSHFVDTLRFLGLEIEAAQASLQTIIAERPFENAKRGVTSDDC
                     ALVNLRLAGGAIAALTLSAVASGPDEPATITIHGEDGALRLIGEELLTAMRGEPFTLA
                     AGDELAPRRGNSAGGAFGSGTYFLGLALRAALDDGDRSALAAAATFDDGLAHQHVLDA
                     ARASSAREGCWIEI"
     gene            complement(2708..6004)
                     /locus_tag="VNN25_24515"
     CDS             complement(2708..6004)
                     /locus_tag="VNN25_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXH94763.1"
                     /translation="MIHRLRVFLVVLSFAVLANAATPPKAAAPSLPDASILKSMQWRE
                     VGPYRGGRADAVVGIDDQPNVYYFGSTGGGVWKTTDGGQSWKPVSDTFFGGTIGAVAV
                     APSDPSVVYAGTGEETIRGNVSPGVGMWKSTDAGKTWTHIGLDDSQQIARIRVHPSNA
                     DLVYVAALGHAFGSNDMRGVYRSRDGGKNWERILFVNRDSGAVDLNMDPSNPRILYAS
                     TWRFRRGAYFFDSGGDGSALWKSTDGGDTWKELSKNKGMPKGTLGIIGVSVSPSNPQN
                     VYAIVEAKEGGVFRSRDGGDTWAKTTDSADLRQRAWYYSRIYADPKDEDAAYVVNVRF
                     HKTKDGGRTWSAINTPHGDNHDLWIAPSDPRRMIEANDGGVNVTTDGGINWTKQDNQP
                     TAQFYRVSVDTDFPYRILGPQQDNTAVRIRHRSGGGGIGPRDWEETAGGESGYIVADP
                     KNPDIVYGGSYGGLLTIYNHRTGEQRDVNPWPDNPMGWGAAGIKHRFQWNFPIFFSPN
                     DPNKIYAASQFLMVSTNAGQSWKTISPDLTRNDPTKEGPSGGPITKDNTSVEYYDTIF
                     YGAESPVEAGVLWVGSDDGLIHVSRDSGAHWEDVSPPKAIMPEWIMINEIDASPLDKG
                     TAYVAATNFKWDDFHPYLYKTDDYGKSWKKIVDGIPATEFTRVIRADPKKRGLLYAGT
                     ERGVYVSYDDGGHWQSLQYKLPIVPIHDLLVHDDALIAATHGRAFWMLDNLEPLRQLT
                     PDIASKPAHIFTPALTWRTGGGGGRGGGGGQGTNPPNGAIIDYYLHGQKVGTKVSLAI
                     LGGDGKVIREYTGTVQAEPAKPAEANGTVGVAPAPPVTPPAPAAGSEKKEGVQSEGAT
                     AEQNPAGEAAEEAGEEGGRRGTDSNKLTEVVNGHNRFTWNLRTNEAKRFPGLIMWSGG
                     TIGPRVIPGTYQARLTVGDQKAETVPFEVKQDPRSTATAADLKAQFDFVTSVHDKLSE
                     VNGQITRIREVRKQLTDVKKRVSDNKAIVAAANDLDKKMTAVEEALYQTKNKSSQDPL
                     NYPIKLNDKLAGVGDSAASGPYAPTAQQIEVRDELVVQIDAQLAKLKAIWDTDLPAFN
                     KLAAGVPVIK"
     gene            complement(6168..>6679)
                     /locus_tag="VNN25_24520"
     CDS             complement(6168..>6679)
                     /locus_tag="VNN25_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXH94764.1"
                     /translation="LLHISEMAHGRVGQVTDVMQIGDEIEVQVVGIEPDSGKIRLSRK
                     PLLPPPTEEEAAAAARAPRRDGPGGGGSDRGGRGGFGDRDRGPRGGGGDRGRSGSGDR
                     GPRRDHDRGPRRDSGPRHEGGPRHEGGPRHEGGPRHEGGAPRFNNESGSTTSSDAPKT
                     GDGDEGGNS"
BASE COUNT         1114 a         2176 c         2130 g         1259 t
ORIGIN      
        1 cgtgccaggc tccgtcgtag aagcttgctt cttcgacgcc gaagatcagg tctgattctt
       61 cgttcgggcc ggcgagggtg aagtcggcgg ggcgggcgag cgcgatgccg cttccctgaa
      121 acggcggcgt cgccaggtcc cacgaaccga tcaatgactc gactacgttc ccgcgaacgg
      181 tgaccgggat cgcattcacg tcaccggttg ccatcgcggc ggcctcactg cgcggatcgc
      241 cgtagatttc ggaaggaaca ccttgctgca cgacgcggcc attgtcgagc accacgacgc
      301 ggtcgctcgc gccgagctcg atcagtgatg acggcgcact gatcaccgtc gtgccgagga
      361 cgcgaagggt gcggtaccac tcgtcgacga tgctgacgct ctcggcgaga ttgagccgct
      421 cgagcatccg atcgccgagg aggatggcag gcttcaagat ctcgatgcgc gcgaggtgtg
      481 cgcgcaccac ctcggtcgat gaaagggagt cgaggcgccg gtcgagaaga tcgccgagcc
      541 gccacttcga cgttgccagc tcgacttcat gctgccggtc ttcgcgatcg agcgtccgct
      601 gccgcgccgc cgcgatgagc agatgtcgca ccgaccagcg ccgcggtgcg tcaatcgacg
      661 agaccgcatg aagcaacggc cgccgccccg ccttcagatc gttgacgacg cgggaaccga
      721 tgcgcacctc acctgagtcc ggccggaccg cgccggccat gatctgcagc agtgtcgaag
      781 caccgcaccc cgccggcccg acgaccgccg tatgcgtcga tctcggaaac gtgagcgtga
      841 cgtcgcgtag cgcgaagccc gcgtaggcga acgacaggtt gtggacggag acgtcgagca
      901 ttgggggatt gtagggggtg gggagtgggg ggtggggagt gggagtcggt tgttgggggg
      961 tcgggggtcg gagaaaaacc aaacgtctca gtttgttgct ccgactcccg actcccgacc
     1021 cccgactccc gactcccgac tcccgccgac tcccacaccc aactccccgc cccccacccc
     1081 cactgtcccg cacggtatgc cacttgcagt cgttctccct gcctcggaaa ctcaacattt
     1141 tcacgcaacc gacggagcat aggtgagcat tcaagcccgg cgtacgccgt tcaaactgcg
     1201 caagctgctg gtcatcgacg atgacgcgaa actgctcgcc tcgtatcgcg acctcctgct
     1261 cccctacggc ttccaggtgc tcaccgcatc cgacggtcgg gacgcgatcc cgctcgtcga
     1321 acagaatccc gacatcctcc tggtcatcct cgatctggct atgccgcgta tggacggccg
     1381 cgagtggctg cgttggttcc gcggctgccg caaagaatcg ccggtgatca tcatctcggc
     1441 ctacaagctg gaggcgagcg atgaggatct gaagccggcc gtgattctgg agaagccttt
     1501 ccacgtcgcc gagcttctcg atctggtcgg cctcttctgc ggtctcggca cgccgatggc
     1561 caacgaagta agctgatcgc agcatggcgc gcgtcggcat catcggcacc ggttggggag
     1621 cgcgcgtgca ggctcccgcg ttccgcgagg cagggctcga agtggttgcg attgcaggac
     1681 acaacgccga tcggacgcgc cgcgcggccg gcgagctcgg gctcgaagcg ttcgacgatt
     1741 ggcgcgcgct gatcgcgtcg aatatcgatc tcgtcacgat cgtaacgccc ccttccgaac
     1801 atctgtcgat ggcctcgact gcgctcgacg ccggcaaaca cgttctaagc gagaagccga
     1861 tggcgctcca cgtggccgaa gcggagcaac tcgtcgccgc agcgcgtcgt cacccggatc
     1921 aactcgcgct catcgatcac gagctgcgct ttctcccctc gtttcgcgcg gcgcgcgagc
     1981 gcatcgccga gctgggcgga gtcaggtatg ccgaggtgcg gtacgcgagt ccgggaagag
     2041 gggatcggag tcgagggtgg aattggtgga gcgacgcgaa acaaggcggc ggaatctggg
     2101 gcgcggtcgg ctcccacttc gtcgacacgc tgcgcttcct cggcctggag atcgaagcgg
     2161 cgcaggcgag tctgcaaacg atcatcgccg agcgcccctt cgagaatgcg aagcgcggcg
     2221 tcacctcgga cgactgcgcg ctggtgaatc ttcgacttgc cggcggagcc atcgctgccc
     2281 tgactctcag cgccgtcgca tcaggtcccg atgagccggc gacgatcacg attcacggcg
     2341 aagatggcgc actgcggctg atcggggaag agctgctcac agccatgcgt ggcgaaccgt
     2401 tcacgctggc cgcgggcgac gagctcgcac cacgacgcgg caactccgcc ggcggcgcgt
     2461 tcggcagtgg aacgtatttt ctcggcctcg cgctccgcgc cgcgctcgac gacggcgacc
     2521 gcagcgcatt ggccgctgct gcgacattcg acgacggcct cgcacaccag cacgtgctcg
     2581 acgctgcacg cgcatcgagt gcgcgcgagg gatgctggat agagatatga atctgcgccc
     2641 atctgcgctc atctgcggat aaaagccttg tccgcagatg gcgcagatga acgcagataa
     2701 agacggctta cttgatgact ggtactcctg cagccagttt gttgaatgca ggcagatccg
     2761 tatcccagat cgctttcagc ttcgcgagtt gcgcgtcgat ctgcacgacc agctcgtcgc
     2821 gcacctcgat ctgctgcgcg gtcggcgcgt atggccctga tgctgcggaa tcgccgacac
     2881 cggcaagctt gtcattgagc ttgatcggat agttcagcgg gtcctgcgag ctcttgttct
     2941 tcgtctgata gagagcctct tcgacggccg tcatcttctt gtcgagatca ttcgcggcgg
     3001 cgacgatagc cttgttgtcg gagacgcgct tcttcacgtc ggtgagctgc ttgcgcacct
     3061 cgcggatgcg cgtgatctgc ccgttcacct cggaaagctt gtcgtgaacg ctggtaacga
     3121 agtcgaactg cgctttcaaa tcggcggctg tggccgtgct gcgcggatcc tgcttcactt
     3181 cgaacggaac ggtctccgcc ttctgatcgc cgactgtcag ccgcgcctga tacgttccgg
     3241 gaatcacccg cggtccgatc gttccgccgc tccacatgat gagaccgggg aagcgcttcg
     3301 cctcgttcgt gcgcaggttc caggtgaagc gattgtggcc gttgacgacc tcggtgagtt
     3361 tgttggagtc ggtgccgcgg cgaccgcctt cttcgcccgc ctcttccgcg gcttcgccgg
     3421 ccggattctg ctcggctgtc gcaccttctg attgaacccc ttccttcttt tcggacccgg
     3481 ctgcgggagc gggcggagtc accggcggcg ccggcgccac accaaccgtt ccattcgcct
     3541 ctgccggctt tgccggctcc gcttgcacgg ttcccgtgta ttcgcgaatg accttgccgt
     3601 cgccgcccag gatggcgagc gaaaccttcg tacccacctt ctggccgtgc aggtagtaat
     3661 caatgatggc gccgttggga ggattcgtcc cctgaccacc cccaccgccg cgccctcccc
     3721 cgccgcccgt acgccaggtc agcgcgggcg tgaagatgtg tgccggcttg gatgcgatgt
     3781 cgggcgtgag ctgccggagc ggctcgaggt tgtcgagcat ccagaacgcg cggccgtgcg
     3841 tcgctgcaat caacgcgtcg tcgtgaacga gcagatcgtg aatcgggacg atcgggagct
     3901 tgtactggag cgactgccag tggccgccgt catcgtacga aacatagacg ccgcgctccg
     3961 tgccggcgta gagaagtccg cgtttcttcg gatcggcgcg gatgacgcgc gtgaactctg
     4021 tcgccggaat cccgtcgacg atcttcttcc agctcttgcc gtagtcatcg gtcttgtaaa
     4081 gatacggatg gaaatcgtcc cacttgaagt tcgtggcggc gacgtaggcc gtgcctttgt
     4141 cgagcgggct ggcgtcgatc tcgttgatca tgatccattc gggcatgatc gccttcggcg
     4201 gcgagacatc ctcccagtgc gcaccgctgt cgcgcgagac gtgaatgagt ccgtcgtccg
     4261 agccgaccca gaggacaccg gcttcgaccg gagattcggc gccatagaaa atcgtgtcgt
     4321 agtactcgac gctggtgttg tccttggtga tcggcccgcc actgggacct tccttggttg
     4381 gatcgttgcg cgtcaggtcg gggctgatcg tcttccagct ctggcccgcg ttcgtcgaca
     4441 ccatcaggaa ctgcgacgcg gcgtagatct tgttcggatc gttcggcgag aagaagatcg
     4501 gaaagttcca ctggaagcgg tgcttgatgc cggccgcgcc ccatcccatc ggattgtcag
     4561 gccacggatt gacgtcgcgc tgctcgccgg tgcggtggtt gtagatcgtc agcaggccgc
     4621 cgtacgagcc gccgtaaacg atgtcgggat tcttcggatc ggccacgatg tagccgctct
     4681 cgccgcccgc ggtctcttcc cagtcgcgcg gaccgatgcc gccgcctccg ctgcgatgac
     4741 ggatacgcac ggcggtgttg tcctgctgcg gccctaggat gcgatagggg aaatcggtgt
     4801 cgacggaaac gcggtagaac tgcgcggtcg gctggttgtc ctgcttcgtc cagttgatgc
     4861 caccgtcggt ggtgacgttc accccgccgt cattcgcttc gatcatccgc cgcggatcgc
     4921 tcggcgcgat ccagagatcg tggttgtcac cgtgcggcgt attgatcgcg ctccacgtcc
     4981 tgccgccgtc tttcgtcttg tgaaagcgga cgttgacgac gtaggccgcg tcttcgtcct
     5041 tcggatcggc gtagatccgc gagtagtacc aggcgcgctg acgaagatcg gccgagtcgg
     5101 tcgtcttcgc ccatgtgtcg ccgccgtcgc gtgagcggaa gacgccgcct tcctttgctt
     5161 cgacgatcgc gtagacgttc tgcggattcg aaggcgaaac gctgacgccg atgatgccga
     5221 gggttccttt cggcatcccc ttgttctttg atagctcttt ccacgtgtcg ccgccgtcgg
     5281 tcgatttcca aagcgccgag ccgtcgccgc cgctgtcgaa gaaatacgcg ccgcgacgga
     5341 aacgccaggt cgaggcatag aggatgcgcg gattcgacgg gtccatgttc agatcgaccg
     5401 cgccggagtc gcgattgacg aagaggatgc gctcccagtt cttgccgcca tcgcgcgagc
     5461 gatagacgcc gcgcatgtcg ttcgacccga aggcatggcc taacgccgcg acgtaaacga
     5521 gatcggcatt cgaaggatgg acgcggatgc gcgcgatctg ctgcgagtca tcgagaccga
     5581 tgtgcgtcca cgtcttccct gcgtcggtcg acttccacat ccccactccg ggcgagacat
     5641 tgccgcgaat ggtctcttcc cccgtcccgg cgtagacaac gctcggatcg gacggcgcca
     5701 ccgccaccgc cccgatcgtc ccgccgaaga aggtatcgga caccggcttc cagctctgcc
     5761 cgccgtcggt tgtcttccac actccgccac cggtcgagcc gaagtagtag acattcggct
     5821 gatcatcgat gccaacgacc gcatccgccc gcccgccgcg atagggacca acctcgcgcc
     5881 actgcatcga cttgaggatc gaagcgtcgg gaagcgatgg tgccgctgcc ttgggcggcg
     5941 tcgccgcgtt ggcaagaaca gcaaaggaga ggacgaccag gaacacccgc agacgatgaa
     6001 tcatgcgcac ttcctccgaa ggagcgcatg tttaccacaa atggaaaggt cgcccgagga
     6061 acgacttcac tccggagcgg ggaaccggac ccgatgacga ggcatccctg agctaaaact
     6121 aaaacgccgc ccgaaggcgg cgttttaatc aattgccttg aatcgaatta cgagttcccg
     6181 ccctcgtcac cgtcacccgt tttcggagca tcggaggagg ttgttgaacc gctctcgttg
     6241 ttgaagcgcg gagcgccgcc ttcgtgacgg gggccacctt cgtggcgagg gccgccttca
     6301 tggcgcggac caccttcgtg gcggggacct gaatcgcggc gcgggccgcg gtcgtgatcg
     6361 cggcgtgggc cgcgatctcc cgagccgctg cgtccgcggt ctccaccacc accgcgcgga
     6421 ccacgatcgc ggtcgccgaa accgccgcgt ccaccgcggt cgctgccacc accacccggg
     6481 ccgtcgcggc gcggggcgcg tgctgcggct gctgcttctt cctcggtcgg aggcggcagg
     6541 aggggcttac gcgacagacg gatcttgccg ctgtccggct cgatgccgac cacctgcact
     6601 tcgatctcgt cgccgatctg catgacgtcc gtcacctggc cgacgcggcc gtgcgccatc
     6661 tcggagatgt gcagcagtc
//
LOCUS       DATLVW010000836         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_283884, whole genome shotgun sequence.
ACCESSION   DATLVW010000836 DATLVW010000000
VERSION     DATLVW010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283884"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VNN25_24525"
     CDS             complement(<1..692)
                     /locus_tag="VNN25_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXH94765.1"
                     /translation="MNEFDYTGHIEQRFPVNIEDEMRKSYLDYAMSVIIGRALPDVRD
                     GLKPVHRRILYGMYEQGNTAGKAYKKSARIVGDVMGKYHPHGDSAIYDSVVRMAQDFS
                     MRYRLVDGQGNFGSVDGDNPAAMRYTEVRLTRLAEELMRDDIDKETVDFVPNYDGTES
                     EPSVLPAKYPNLLVNGSSGIAVGMATNIPPHNLGEVIDACLMVMENPAASVKELMTVM
                     PGPDFPTAGFIHG"
     gene            complement(763..>2182)
                     /locus_tag="VNN25_24530"
     CDS             complement(763..>2182)
                     /locus_tag="VNN25_24530"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXH94766.1"
                     /translation="VKIRDPKFSSQTKDKLVSSHVKTWVEQVVGERLGEFLEENPKEV
                     RRIVDKVIEAARAREAARKARDLTRRKGALDSASLPGKLADCQERDPRYCEIYFVEGD
                     SAGGSAKQGRDRRFQAILPLKGKILNVERARLDKMLSSEEIRLMITALGTGIGAGDFD
                     IAKLRYHKIIIMTDADVDGSHIRTLILTFFFRYMREVIDRGYLFIAQPPLFKVAQGKK
                     DSYIKDEGELSRFLLNRVSENIEVTPQAGLSLKGDPLVNMLQRMEDYRSHAQKLQSRG
                     IPREAVDVLFAEGFHDRSALGDPERLASLVKALEAKGFEQVRLMTDEETETPTVRFTV
                     GGNGTGKKVVVNSELLSTYEYRQMSKSFELLSGFGLPPYTLKHNDASDVIVTIDDLID
                     RVYDLAKKGLAISRYKGLGEMNPEQLWETTMDPERRRLLQVKIEDGVGAEELFTVLMG
                     DQVEPRRVFIQDNALEVKNLDI"
BASE COUNT          370 a          639 c          706 g          467 t
ORIGIN      
        1 ccgtggatga aaccggcggt cggaaaatcg ggaccgggca tgacggtcat cagctctttg
       61 accgacgcgg ccgggttctc catcaccatc aggcaggcgt cgatgacttc gccgaggttg
      121 tgaggcggga tgttggtggc catgccgact gcgattccgg acgagccgtt gaccagcagg
      181 ttgggatact tcgccggcag aaccgacggc tcgctctcgg tgccgtcgta attcggcacg
      241 aagtcgaccg tctccttgtc gatgtcgtcg cgcatcagtt cttcggcgag gcgcgtcagg
      301 cggacttcgg tgtaacgcat ggccgccggg ttgtcgccgt cgaccgagcc gaagttcccc
      361 tggccgtcga cgaggcggta gcgcatcgag aagtcctgcg ccatgcgcac gaccgagtcg
      421 tagatcgcgg agtcgccgtg cgggtggtac ttgcccatca cgtcgccgac gatacgggcc
      481 gacttcttgt acgccttgcc tgccgtgttt ccctgctcgt acatcccgta gaggatgcgg
      541 cggtgcaccg gcttgagtcc gtcgcgaacg tccggaagcg cgcgtccgat gatgacggac
      601 atcgcgtagt cgagatacga cttgcgcatc tcgtcttcga tgttgaccgg gaagcgctgc
      661 tcgatgtgtc ctgtgtagtc gaattcgttc atgtggtgtg gttggttact cggttattga
      721 gctaccggct gcgatggtca gcaacgaagc agcccagcaa cctcagatat ccagattctt
      781 cacttccagc gcgttgtcct gaatgaacac gcgccgcggt tcgacctgat cgcccatcag
      841 caccgtaaac agctcctcgg cgccgacgcc gtcttcgatc tttacctgca gcaggcggcg
      901 gcgctcggga tccatcgtcg tctcccagag ctgctcggga ttcatttcgc cgaggccttt
      961 gtagcggctg atggcgaggc ctttctttgc caggtcgtaa acgcggtcga tcagatcgtc
     1021 gatcgtgacg atcacgtcgg atgcgtcgtt gtgcttgagt gtgtacggcg gcagaccgaa
     1081 gccggaaaga agctcgaacg acttcgacat ctgccggtac tcgtaggtcg aaagcagttc
     1141 gctgttgacg accaccttct tgcccgtgcc gttgccgccg accgtgaaac gaaccgtggg
     1201 agtctcggtt tcctcgtccg tcatcaggcg cacctgctcg aagcctttcg cttcgagtgc
     1261 cttgacgagt gaggcgagcc tctccggatc accgagcgcg ctgcgatcgt ggaaaccttc
     1321 ggcaaagagc acgtcgaccg cttcgcgtgg gatgccgcgc gactgcagct tctgcgcgtg
     1381 gctgcgataa tcctccatcc gctggagcat gtttactagc gggtcgcctt tcagcgaaag
     1441 cccggcttgc ggcgtgacct cgatgttttc cgagacgcgg ttgagaagaa agcgcgacag
     1501 ctcgccttca tccttgatgt acgagtcctt cttcccctgc gctaccttga acagcggcgg
     1561 ctgggcgatg aagaggtagc cccggtcgat gacctcgcgc atgtagcgga agaaaaacgt
     1621 gaggatcagc gtgcggatgt gcgaaccgtc gacgtcggcg tccgtcatga tgatgatctt
     1681 gtgatagcgg agcttggcga tatcgaaatc gccggcgccg atgccggtgc cgagggcggt
     1741 aatcatcagg cggatttctt cggaagagag catcttgtcg agacgtgcac gctcgacgtt
     1801 gaggatcttg cccttgagcg gcaggatggc ctggaagcgg cggtcgcgac cctgtttggc
     1861 agagccgccg gccgaatcgc cttcgacgaa atagatttcg cagtagcgtg gatcgcgctc
     1921 ctggcagtcg gcgagtttgc ccgggagtga ggcggagtcg agcgcgcctt tgcgtcgggt
     1981 gaggtcgcgg gctttgcgcg cagcctcgcg ggcgcgagca gcctcgatga ccttgtcgac
     2041 gatgcggcgg acttccttcg ggttctcctc gaggaactcg ccgaggcgtt cgccgacgac
     2101 ctgctcgacc caggtcttca cgtgggacga gacgagcttg tctttcgtct gcgatgagaa
     2161 cttcggatcg cggatcttga cg
//
LOCUS       DATLVW010000837         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_283933, whole genome shotgun sequence.
ACCESSION   DATLVW010000837 DATLVW010000000
VERSION     DATLVW010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283933"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VNN25_24535"
     CDS             complement(<1..846)
                     /locus_tag="VNN25_24535"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXH94767.1"
                     /translation="MSGRLGVAAALLLATNVFAGSQVIATHAAIATSSPAATQIGLRV
                     LKSGGNAIDAAVAVSFALSVLEPQATGLGGGGFLTYYDAESGGVWTLDFRETAPAAAK
                     VLANKGAPSACVPGFLAGLDMMHRRFGSRPWRDMVTPAQLLAKDDLAKTLQRIGEQGA
                     RDFYDGDLAHKLIEAVQAAAGSFAYRDLREYKPVWRAPVRIDFRGYEIYAPAPPAAGG
                     LALGTVLKIVSGLDLGAGGLQSAKALHLLLEAERRAAIDRDKFLGDPAGGRVPMHDLL
                     SDERAA"
     gene            complement(852..1637)
                     /locus_tag="VNN25_24540"
     CDS             complement(852..1637)
                     /locus_tag="VNN25_24540"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXH94768.1"
                     /translation="MSNLLVIDVGNTNIVLGVFRGNGLVSSWRLATARERTSDEYGIL
                     ARQLIGETFSDAFEGAIVSSVVPPLNHSVASMVRKFFGIDAFFVEPGVKTGIAIHTDN
                     PQEVGADRIVNCVAAHAEYGGPAIIVDFGTATTFDVVTHDARYIGGVIAPGLTISAEA
                     LFARAARLPRVDIRRPESVIGTNTIVNMQSGIYFGYLGLVDGILGRMKREVPDVKAVV
                     ATGGLASLLAPDSEHIEYVDDDLTLKGLKIIYERNRAPRRGRK"
     gene            complement(1856..1945)
                     /locus_tag="VNN25_24545"
     tRNA            complement(1856..1945)
                     /locus_tag="VNN25_24545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1909..1911),aa:Ser,seq:gga)
     gene            complement(2042..2131)
                     /locus_tag="VNN25_24550"
     tRNA            complement(2042..2131)
                     /locus_tag="VNN25_24550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2095..2097),aa:Ser,seq:cga)
     gene            complement(2135..2611)
                     /locus_tag="VNN25_24555"
     CDS             complement(2135..2611)
                     /locus_tag="VNN25_24555"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXH94769.1"
                     /translation="MVEAEAMDQAEWMTEALALAGQAGAAGEIPVGAVIVDRDGQIIG
                     RGSNRRESGDPLGHAELIAIRQAAEVIGDWRLEGTTMVVTLEPCAMCAGALVNARVER
                     LIFGAFDPKAGFCGSLGNLVQDARLNHRLEVISGVEAEASRKLLQGFFQGLRKRLD"
     gene            complement(2696..2772)
                     /locus_tag="VNN25_24560"
     tRNA            complement(2696..2772)
                     /locus_tag="VNN25_24560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2736..2738),aa:Arg,seq:acg)
BASE COUNT          577 a         1015 c          927 g          554 t
ORIGIN      
        1 cgcggcgcgc tcgtcagaga gaagatcgtg catcggcacg cgtccgccgg caggatcacc
       61 gagaaatttg tcgcggtcga tcgcggcacg ccgttcggct tcgagcagaa ggtgcaacgc
      121 cttcgctgac tgcaagccgc ccgcgccgag atcgagtccg ctgacgatct tcaacaccgt
      181 accgagcgcg agacctccgg cggccggagg cgcaggcgca tagatctcgt aaccgcgaaa
      241 atcgattcgc accggcgcgc gccagaccgg tttgtattcg cgcagatcgc gatacgcaaa
      301 cgagccggct gcggcctgaa cggcttcgat cagtttgtgc gccaggtcgc cgtcgtagaa
      361 gtcgcgcgct ccctgctctc cgattcgttg gagcgtcttc gccagatcgt ccttcgccag
      421 gagttgcgcc ggcgtgacca tatcgcgcca cggccgcgaa ccgaaacgac gatgcatcat
      481 gtccagcccg gccaggaacc ccggcacgca cgccgatggc gcgcccttgt tcgcgagcac
      541 cttcgccgcg gcaggcgcgg tctcgcgaaa atcgagcgtc cagacaccgc cgctttcggc
      601 gtcgtagtag gtcaggaatc cgcctccgcc gaggccggtg gcctgcggtt cgagaacgga
      661 aagtgcgaag gagacagcaa ccgcggcgtc gatcgcgttc ccgccgctct tcaaaacgcg
      721 caagccgatt tgcgtcgcgg cgggcgaaga ggtcgcgatc gcggcgtgcg tggcgatgac
      781 ctgcgaaccg gcgaagacgt tcgtcgcaag caggagcgcg gctgcaactc caagcctgcc
      841 ggacacaagc ctcacttccg tccccggcgc ggggctcgat tgcgctcgta gatgatcttc
      901 agtcccttga gtgtgagatc gtcgtccacg tattcgatgt gctcggagtc gggagcgagc
      961 agcgaggcca atccgccggt agcgacgacg gctttgacat ccggcacctc gcgtttcatg
     1021 cgcccgagaa tgccgtcgac gagcccgagg tagccgaagt agatgcccga ctgcatgttg
     1081 acgatcgtat tcgtcccgat gacagactcc ggcctccgga tgtcgacccg cggtagccgc
     1141 gccgcgcgcg cgaacagcgc ctccgcggag atggtcaggc ccggcgcgat cacgccgccg
     1201 atgtagcgcg catcgtgcgt gacgacatcg aaggtcgtgg ccgtgccgaa gtcgacgatg
     1261 atcgccggac cgccgtactc tgcatgcgcg gccacgcagt tgacgatgcg gtcggcccct
     1321 acttcctgcg gattgtcggt gtgaatggcg atgccggtct tgactcccgg ctcaacgaag
     1381 aaggcatcga tcccgaagaa cttgcgcacc atcgaggcaa cggagtgatt gagcggcggc
     1441 acgaccgacg agacgatcgc cccctcgaag gcatcggaga acgtctcgcc gatgagttgt
     1501 cgcgccagga ttccgtattc gtcggaggtc cgttcacgcg ccgtggccag ccgccaggaa
     1561 ctgacaagtc cgttcccgcg aaaaacgccg agaacgatgt tggtgttgcc gacgtcgatg
     1621 acgaggaggt tcgacacggt gctaggttag caggcgttgc tgatgttgat gctcacagcc
     1681 agcaccacag agacacagag gccacagaga aaagaactac tcagtgatct cggtgtctct
     1741 gtggtgctgg ttttcttttg aaaacacatc acggccgctt gtaaaccgcc ccatccttca
     1801 tcacgaaggc gacgcgctgc cagggcggcg caccaatgaa gccggatgag tccagtggcg
     1861 gagagagtgg gattcgaacc cacggtacgg gttaacgtac acacgctttc caagcgtgcg
     1921 ccttaagcca ctcggccatc tctccgtatg tcaacgcacc ctggcaactc actctgaacg
     1981 tgaatctctg cgcaaatcct gaagcaggct tggccaatca acgctgccgg ccgtgccggc
     2041 ctggcggaga gggggggatt cgaacccccg atacccgtga aggtataacg gttttcgaga
     2101 ccgccgcgtt caaccactct gccacctctc cgtgtcaatc cagccgcttg cgaagacctt
     2161 gaaagaaccc ctgcagaagt ttccgcgatg cctcggcttc cacaccgctg atcacttcca
     2221 gccggtgatt gagcctcgca tcctggacga gatttccgag cgagccgcag aagcccgcct
     2281 tcggatcgaa tgctccgaag atcagcctct cgacccgtgc gttgacgagc gctccggcgc
     2341 acatcgcgca cggctcgagc gtcaccacca tcgtcgtccc ttccagacgc cagtcgccga
     2401 tgacttcggc cgcctgacgg atggcgatca gctccgcgtg gccgagcgga tcgcccgact
     2461 cgcgccggtt gctgccgcgg ccgatgatct gcccgtcgcg gtcgacgatg accgcgccca
     2521 cgggaatctc gccggctgcg cccgcctgtc ccgcgagcgc gagcgcttcc gtcatccact
     2581 cagcctgatc catggcctcg gcttccacca tcggcttcta cctggggttc tacctgattc
     2641 agcgttcaaa gagtagaacg gcgggctcgc tgcccgccgt cgatttgttc ctgattggtg
     2701 cacccggcaa gactcgaact tgcaaccttc tgatccgtag tcagatgctc tatccaatta
     2761 agctacgggt gcgagcaagc gacgttaacc gtccgttcgg aaaaggcatt ccggtgcggc
     2821 cgcggcgcgg gggcgcattc taagtccgcc tccgccgccg aggcaagcaa aagagcttta
     2881 cttcgtccac tcttcttgcg gtagactttc cttgctcagc agcaggttca caccaccacg
     2941 gttggcgact gcctgcctac ccttccccag ccaaccgtgg tggtgtgatg ttttcaatca
     3001 tgccgctcga caagaaatcc ttctacgcgc gcaccatgca cactccggac tcgccggagc
     3061 gcgccgcgca tcg
//
LOCUS       DATLVW010000838         5226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_369342, whole genome shotgun sequence.
ACCESSION   DATLVW010000838 DATLVW010000000
VERSION     DATLVW010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369342"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..2255
                     /locus_tag="VNN25_24565"
     CDS             27..2255
                     /locus_tag="VNN25_24565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXH94770.1"
                     /translation="MPEKIRRYVLKQPAKAQKQYRVRYEDELNQEQLDVVMAGEGPML
                     VIAGAGSGKTRALTYRVSRLIEDGVDPSDILLLTFTNKSAREMLSRVEQLVTIDTRRI
                     WGGTFHSIGNRLLRQHATAIGYRSSFTILDAEDAKEMMESAISSLGIKTLEKRFPKGD
                     VLIDIYSYLINTRTPLELHLENNYPHFGVYRDEMINVFRRYKERKREANAMDFDDLLV
                     NWKLLLDDHPEAGEALKRRYRYILVDEYQDTNKLQAEVVDAMASVRRNVMVVGDDAQS
                     IYSFRGASFENIITFPLRFPEAKIYKLETNYRSTRQILSLANASIAQNRFQFKKQLHA
                     VRGDGPDPAVVGVDDVFEQASFVAQRILELRDEGEPLSDIAVLYRSHYQSLELQMELS
                     RRLIPYEIRSGVRFFEQAHIKDVIAYLKIVTNPRDELSWKRVMKLYPKVGEKTAAEVW
                     ERIGKDANPLETFLRGSGVGGRTSEKGSYASDDGSDTRPPTRDPRTAISKSLKSLATI
                     LGVIGSESMQHNPSESIRLIVERGYGDYARAKFANSQARLDDLEQLSQYALRYDDTNL
                     FLDEVALSNPIAGEDVAVVGPEDEKVVLSSVHQAKGLEWRIVFVIWLADGRFPSQRAL
                     RVPGGIVHVQPKELHEAFELTDALMKKDDLAIEELPTELRDADAAPTSSSPEVEIVIP
                     GEEEERRLFYVAVTRAKQELYLTFPVMARDRGGLDILMEPSRFIRELPGDSYEKWVIG
                     AE"
     gene            complement(2360..2764)
                     /locus_tag="VNN25_24570"
     CDS             complement(2360..2764)
                     /locus_tag="VNN25_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94771.1"
                     /translation="MYGARPDVRGVQPDVCGARPGVRSVEPDLRGVHPHVSGVQPDVC
                     GAQPGVRGVEPDVRGVQPDVRGARPDVCGAQPDVRGAQPDVCGVEPDVCGVEPDVRGA
                     QPEVCGAQPEVCGIQPDVCGVQPDVYIVPLGQ"
     gene            complement(3385..3852)
                     /locus_tag="VNN25_24575"
     CDS             complement(3385..3852)
                     /locus_tag="VNN25_24575"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH94772.1"
                     /translation="MRDNWTIRPAVPSDREFLFRLHATTMRSVIEQTWGWDEQWQRND
                     FAERFLSVLFYVVEARAQPIGALCLEQSPDCLYIRELQLLPEQQGHGIGTAIVDMVLD
                     EARKQSLPVKLSVVPANDRAQRLYERLGFRVAAVELPFIRMVYDAVGAREGPN"
     gene            complement(3871..4638)
                     /locus_tag="VNN25_24580"
     CDS             complement(3871..4638)
                     /locus_tag="VNN25_24580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXH94773.1"
                     /translation="MLTIVLAAAVAATLAKPAPPADLKTPPADAQKSADGLLSKQLVP
                     GTAGEHPTAADFIHLRYAVWKASDGSVVDYTRVSYSFIEMAKLLPGMREMFEQMTPGE
                     QRRAWIPSSLGGGRIAEGETFVVEGQLIDVVHPPVTPPDVAAPPADATKTSSGLAYKI
                     LRPGTGTRHPARRDTVTVHYSGWTTDGQMFDSSIVRGEPAEFRLDEVIPGWTEGLELM
                     TEGEQVRFWIPERLAYKGKSDKPKGMLVFDVELVKVK"
     gene            4654..>5226
                     /gene="pepE"
                     /locus_tag="VNN25_24585"
     CDS             4654..>5226
                     /gene="pepE"
                     /locus_tag="VNN25_24585"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HXH94774.1"
                     /translation="MRLLLISSSNVHGYGYLDQPEPAIRAFLGGAHRVAFVPFAAHDH
                     AAYTTKMRERLARMSLEVISIEELDNADAVFVGGGNTFRLLKTLYERNLLDIVRERVR
                     GGLPYIGSSAGTVITAPTIRTTNDMPIVYPPSFDSLGLVTFQINPHYLDADPSSTHKG
                     ETREDRIREFHEENERPVVGLREGSMLRVSD"
BASE COUNT          991 a         1703 c         1611 g          921 t
ORIGIN      
        1 ctcccccacc ctgctacgct tcccccgtgc ccgagaagat ccgacgttac gtcctgaaac
       61 aaccggcgaa ggcgcagaag cagtatcgcg tccgctacga agacgagctg aatcaggagc
      121 agctcgatgt cgtcatggcc ggcgagggac cgatgctcgt catcgccggc gccggcagcg
      181 ggaagacgcg cgccctcacc tatcgcgttt cgcgtctcat cgaagacggc gtcgacccgt
      241 ccgacatcct cctgctcacg ttcacgaaca agtcggcgcg ggagatgctc agccgcgtcg
      301 agcagctcgt cacgatcgac acgcgacgca tctggggcgg gacgtttcac tcgatcggga
      361 accgcctgct gcgccagcac gcgacggcga tcggctatcg atcgagcttc acgattctcg
      421 atgccgagga tgcgaaggag atgatggaat cagcgatttc ctctcttggg atcaagacgc
      481 tggagaagcg ttttccgaag ggcgacgtcc tcatcgacat ctattcgtac ctcatcaaca
      541 cacgcacgcc gctggagctg cacctcgaga acaactaccc gcacttcggt gtctaccgcg
      601 acgagatgat caacgttttc cgccgctaca aggagcggaa gcgcgaagcg aacgcgatgg
      661 atttcgacga tctgctcgtc aactggaagc tgctgctcga cgatcatccc gaagcgggcg
      721 aagcgttgaa acgccgctat cgctacatcc tcgtcgacga atatcaggac acgaacaagt
      781 tgcaggcgga ggtcgtcgac gccatggcgt cggtgcgacg gaacgtgatg gtggtcggcg
      841 acgacgcgca atcgatctac tcgtttcgcg gcgcatcgtt cgaaaacatc atcacgtttc
      901 cactccgatt ccccgaagcg aagatctaca agctggagac taactaccga tcgacgcgac
      961 aaatcctcag cctcgccaac gcctcgattg cgcagaatcg cttccagttc aagaagcaat
     1021 tgcacgcggt ccgcggcgat ggtcccgacc cggcggtagt cggcgtcgac gatgttttcg
     1081 agcaggcctc gttcgtcgcc caacgcatcc tcgagctgcg cgacgaaggc gagcccctct
     1141 ccgatatcgc cgtcctctac cgctcgcact accagtcgct cgagctgcag atggagctct
     1201 cgcggcgcct gatcccgtac gagatccgca gtggcgtccg ctttttcgaa caggcccaca
     1261 ttaaagacgt gatcgcctac ctgaagatcg tcacgaatcc gcgcgacgag ctgtcgtgga
     1321 agcgcgtgat gaagctgtat ccgaaggtgg gcgagaaaac ggcagccgag gtgtgggaac
     1381 ggatcgggaa ggacgcgaat ccgctggaga cgtttctgcg ggggtcggga gtcgggggtc
     1441 ggacgtcgga gaaaggcagc tacgcttccg acgatggctc cgacacccga cccccgacgc
     1501 gcgacccccg cacagccatc tcgaagagtc tcaagagcct cgccaccatc ctcggtgtca
     1561 tcggcagcga gtcgatgcaa cacaatccgt cggagtcgat ccgcctcatc gtggagcgcg
     1621 gctacggcga ctacgcccgc gcaaagttcg ccaactcgca ggcgcggctg gacgatctcg
     1681 agcagctctc gcaatacgcg ctccgctacg acgacacgaa cctcttcctc gatgaggtcg
     1741 cgctgtcgaa tccgatcgcc ggcgaggatg tcgctgtcgt cggccccgag gatgagaagg
     1801 tcgtcctctc gtcggtgcac caagccaaag gactggagtg gcgcatcgtc ttcgtgatct
     1861 ggctcgccga cggccggttt ccgtcgcaac gggcgctgcg cgttcctggc ggcatcgtgc
     1921 acgtgcagcc gaaggagctg cacgaagcct tcgaactgac ggatgcgctg atgaaaaagg
     1981 acgacctcgc gatcgaagag ctgccgaccg agcttcgcga cgccgacgcc gcgccaacgt
     2041 ccagctcccc cgaggtcgag atcgtgattc ccggcgaaga ggaagaacgc cgcctcttct
     2101 acgtcgccgt aacgcgcgca aaacaggagc tctacctcac cttccccgtc atggcccgcg
     2161 accgcggcgg cctcgacatc ttgatggagc cctcgcgctt catccgcgag ttgccggggg
     2221 attcgtatga gaaatgggtg attggtgcgg agtagtttct tgcacactcg acgactctca
     2281 gctaacaccc agtacagcgc cactccgtcg taataactta ctgctcgcgc ccgatgattg
     2341 tccgcgccgg ctgagccact cactgtccga gcgggacgat gtacacgtcc ggttgaacgc
     2401 cgcacacgtc cggctggatg ccgcacacct ccggctgggc gccgcacacc tccggctggg
     2461 cgccgcgcac gtccggctcg acgccgcaca cgtccggctc gacgccgcac acgtccggct
     2521 gggcgccgcg cacgtccggc tgggcgccgc acacgtccgg ccgggcgccg cgcacgtccg
     2581 gctgaacgcc gcgcacgtcc ggctcgacgc cgcgcacgcc cggctgggcg ccgcacacat
     2641 ccggctgaac gccgctcacg tgcggatgaa cgccgcgcag gtccggctcg acgctgcgca
     2701 cgcccggccg ggcgccgcac acgtccggct gaacgccgcg cacgtccggc cgggcgccgt
     2761 acacatccag cccgactgcg cagcaggtcg tcccacgttt gcgcggaccg gacgacccac
     2821 tgcagaagac taacccgcgc ttctgccact cctatagcga ctctttccaa tcgagcgcga
     2881 ttgggagcga gtgaaacgta gcgcaggcat tcctgcctgc tcccgtcggg cattcctgcc
     2941 cggcgggtat ttcgaggccg ccacttctca ggagcatcac aattcaccgt cgtcgcgacg
     3001 cccactggcc ggccagagct gagcgacatt cgtggccgag gactgctaaa tccaacacca
     3061 ccgccgggca gggacgcccg gcggtagcag gcaagaatgc ctgcgctacg tcttccccgt
     3121 catggcgcgc gaccgcggcg ggctcgacgt gctgatggaa ccctcgcggt tcatccgcga
     3181 attgccggga gattcatacg agaagtgggt gattggcagc gagtgatatc aatttcaaaa
     3241 aaaacccacc acagaggcac tgagaacaca gagaacgcct ttggttgcgc ctaacggatc
     3301 tgcgcctcac cggcgcggcc cgcgacgcgc tatgggaacg cactcgcgat gccgctgccg
     3361 agcgggccac gtccggggca ggcgctagtt gggcccctct cgcgctccaa cggcatcgta
     3421 aaccatccgg atgaacggca gttcgacagc cgcaacgcga aaacccagcc gctcgtagag
     3481 ccgttgtgcg cggtcattcg ccggcacaac cgagagtttt accgggagac tctgcttcct
     3541 cgcttcatcc aggaccatgt cgacgattgc cgtaccaatg ccgtgtccct gttgttcagg
     3601 gagtagttgc agctcccgaa tgtaaaggca atccggactt tgctcgaggc atagcgctcc
     3661 gatcggctgc gcgcgcgcct cgacaacgta gaacaggacc gaaaggaacc gctccgcgaa
     3721 atcgtttcgc tgccactgtt cgtcccagcc ccatgtttgc tcgataacgc tgcgcatcgt
     3781 cgttgcgtgg agccgaaaaa ggaactctcg atctgaggga actgcaggtc ggattgtcca
     3841 gttgtcgcgc atgtcagagc ggcccaacta ttacttcacc ttcaccaact ccacatcgaa
     3901 caccagcatc cccttcggct tgtccgactt gcccttgtac gcgaggcgct cggggatcca
     3961 gaagcggact tgttcgcctt cggtcatcag ttccaggcct tcggtccagc cggggattac
     4021 ttcgtccagg cggaactcgg cgggctcgcc gcggacgatg gagctgtcga acatctggcc
     4081 gtcggtggtc cagccgctgt agtggacggt gaccgtgtcg cggcgggcgg gatgtcgcgt
     4141 tcccgttccc ggacgcagga tcttgtaggc gaggccggat gaggttttcg tcgcgtcggc
     4201 aggtggcgcg gcgacatcgg gaggcgtcac cggaggatgg acgacgtcga tgagttgtcc
     4261 ttcgacgacg aacgtctccc cttccgcgat tctgccgccg cccagggatg acgggatcca
     4321 ggcgcggcgc tgttcgccgg gcgtcatctg ctcgaacatc tcgcgcattc cgggcagcag
     4381 cttcgccatc tcgatgaagg agtagctgac gcgggtgtag tcgacgaccg aaccatccga
     4441 ggctttccag acggcgtagc gaagatgaat gaaatcggcg gcggtgggat gctctcccgc
     4501 ggtgccggga acgagctgct tgctgagcaa cccatccgcg gatttctgcg cgtcagcggg
     4561 cggcgtcttg aggtcggcag gcggagcggg cttggcgagc gtggcagcga cggcggcggc
     4621 aaggacgatg gtaagcatta gactccgcac aacatgcgcc ttcttctgat ctcttcctca
     4681 aacgtacacg gctacggcta tctcgatcag cccgagcccg cgatccgcgc cttcctcggc
     4741 ggcgctcacc gcgtggcttt cgtgccgttc gcggcgcacg atcatgcggc gtacacgacg
     4801 aagatgcgcg agaggctggc gcggatgtct ctcgaagtca tcagcatcga agagctcgac
     4861 aacgctgacg cggtctttgt cggcgggggg aacacgttcc ggctgttgaa gacgttgtat
     4921 gaacgcaatc tgctcgacat cgtccgcgag cgcgtccggg gtgggctccc ctacatcgga
     4981 tcgagtgcag gaacggtcat caccgcgccg acgatacgga cgacgaacga catgccgatc
     5041 gtttatccgc cgtcgtttga ttcgttgggc ttggtcacct tccagatcaa tccacactat
     5101 ctcgatgctg atccgtcgtc gacgcacaaa ggggaaacgc gcgaggatcg gatccgcgag
     5161 tttcacgagg agaacgagag gccggtcgtt gggctgcgcg aggggtcaat gcttcgggtt
     5221 tcggac
//
LOCUS       DATLVW010000839         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_118142, whole genome shotgun sequence.
ACCESSION   DATLVW010000839 DATLVW010000000
VERSION     DATLVW010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118142"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2123
                     /locus_tag="VNN25_24590"
     CDS             <1..2123
                     /locus_tag="VNN25_24590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94775.1"
                     /translation="TRTFSVCSPDAPVIAVVGTNTSGQTYTVSWTALPAITSYELQEA
                     ATAAFDNPTSFTVAGTSRTFTKSATIATAFFYRVRALGGCSPNGGPFSASERVVIVPI
                     PPINSQDNSATVDINNKKVVVQNVFIPGQGPATPYSAATDEPWLTVSPATGILLPQGI
                     TLVVSADPADLPNGTFTATITLTLGSSAKLGALDAKPTVSVPVSISVVTPVSPANLST
                     PPANSLIIPSVGHLDGINSQWRSDVRITNTAQQKINYLLRFTPAGGDSSQIKQTQISI
                     AANDTTALDDIVKNWYGVGALGDAANGVLEVRPLNTSGKGAPNAEDVNVSLVTVASSR
                     TFNQSAGGTLGQYIPAIPFANFIGKVISGDARAGILSMQQIAQSAAYRTNLGIVEAAG
                     KPVSVLVSVFDAAGKNLLDLPIDLKAGEQQQLNSFLGTKGITLTDGRIEVKVTGGDGK
                     VIAYASIVDNKTTDPLLVTGTPLAQTPSRSYVLPGVADLSNGSANWRSDLRVFNAGTD
                     PQYVTMTLYPQTGSTSSDPISRSVSINPGEITILDNILASRFGLANIGGALHVDTLTD
                     ANLVVTGRTYNQIEGGGTYGQFIAAVTPADAVGKGGRTLNILQVEDSTRYRTNLGIAE
                     VSGKPATVEVSVLLPDAKVAPKLQIPLAANEYRQFRVLQQLGVGTAYNARITIRVVDG
                     DGRIAAYGSVVDMQTQDPTYVPAQ"
BASE COUNT          423 a          815 c          581 g          348 t
ORIGIN      
        1 ccacgcgcac gttcagtgtc tgctctcccg acgcgccggt catcgccgtc gtcggaacca
       61 acacctccgg ccagacctac accgtcagtt ggaccgcgct tcccgccatc accagctatg
      121 agttgcagga agcggcgacc gccgccttcg acaaccccac ttcgttcacc gtcgcaggta
      181 cgtcgcgaac cttcacgaag agtgcgacga tcgcgactgc gttcttctac cgcgtccgcg
      241 ccctcggcgg atgcagtccg aacggcggac ccttctccgc atccgagcgc gtcgtcatcg
      301 ttcccatccc gccgatcaac tcgcaggaca acagcgccac ggtcgacatc aacaacaaga
      361 aggtcgtcgt acagaacgtc ttcatcccgg gccagggtcc ggcaacgccg tacagcgcgg
      421 cgaccgacga gccgtggttg acggtctccc ctgccaccgg gattctcctg ccgcagggaa
      481 tcacgttagt ggtcagcgcc gatcccgccg acctgccgaa tggcacgttt accgccacga
      541 tcaccctcac gctcggcagc tccgcaaagc tcggcgcgct cgacgcaaaa ccgaccgttt
      601 cggttccggt ctccatcagc gtcgttacgc ctgtctcgcc cgccaatctc tcgaccccgc
      661 ccgcgaattc gctgatcatc ccctcggtcg gccatctcga cggcatcaac tcgcagtggc
      721 gctccgacgt ccgcatcacg aacacagccc agcagaagat caactatctg ctgcgcttca
      781 cgcccgcggg cggcgattcc tcgcagatca aacagacgca gatctcgatc gcagccaacg
      841 acacaaccgc gctcgatgac atcgtcaaga actggtacgg cgtcggcgcg ctcggcgatg
      901 ctgccaacgg cgttctcgaa gtccgcccgc tgaacacgag cggaaaaggt gcgcccaacg
      961 ccgaagacgt caacgtctcg ctcgtcaccg ttgcctcgag ccggacgttc aaccagtccg
     1021 caggtggcac gctcggccag tacatcccgg ccattccgtt cgccaacttc atcggcaagg
     1081 tcatctccgg cgatgcgcgc gccggaattc tcagcatgca gcagattgcg cagtccgcgg
     1141 cataccgcac gaacctcggc atcgtcgaag ctgccggcaa accggtctcc gtgctcgtca
     1201 gcgtcttcga tgcggcgggc aagaatctgc tcgaccttcc gatcgatctc aaggccggcg
     1261 agcagcaaca gctcaactcc ttcctcggga cgaagggcat cacgctgacc gatggccgca
     1321 tcgaggtgaa agtcaccggc ggcgacggaa aggtgatcgc ctacgcctcc atcgtcgaca
     1381 acaagaccac cgatccgctg ctcgtcaccg gaacgccgct tgcgcagact ccgtcgcgaa
     1441 gctatgtcct gccgggcgtg gccgacctca gcaacggctc cgccaactgg cgctccgacc
     1501 ttcgcgtctt caacgccggc accgatcccc aatacgtcac gatgacgctc tatccccaga
     1561 ccggaagcac cagcagcgat cccatctcgc gatcggtgtc gatcaatccg ggcgagatca
     1621 cgatcctcga caacatcctt gcttcacggt ttggcctggc caacatcggc ggcgctctgc
     1681 acgtcgacac cctgacggac gcgaacctcg tcgtcaccgg ccgcacctac aaccagatcg
     1741 agggcggcgg cacctacgga cagttcatcg ctgcggtcac tcccgccgat gccgtcggta
     1801 aaggcggccg cacgctcaac atcctgcagg tcgaagactc cacccgctac cgcaccaacc
     1861 tcggcatcgc cgaagtcagc ggcaaaccgg ccaccgtcga agtatcggtg ctcctccccg
     1921 acgccaaagt cgcacccaag ctgcagatcc cgctggccgc gaacgaatac cgccagttcc
     1981 gcgtcctaca gcaactcggc gtcggcaccg cctacaacgc ccgcatcacc atccgcgtcg
     2041 tcgacggcga cggccgcatc gccgcctacg gttccgtcgt cgacatgcag acacaggatc
     2101 cgacctacgt gccggcgcag tgatttcgat cgccggcgct cttcaaacgg ggagcgccgg
     2161 cggaaac
//
LOCUS       DATLVW010000840         7363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_613160, whole genome shotgun sequence.
ACCESSION   DATLVW010000840 DATLVW010000000
VERSION     DATLVW010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613160"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VNN25_24595"
     CDS             <1..199
                     /locus_tag="VNN25_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94776.1"
                     /translation="HIEGTFIGFEVPAGTSDVRVVYRPMSFYGSCAAALLTVLVLWFW
                     PVRKVAAEPAAEQPSSRRRYA"
     gene            complement(238..1215)
                     /locus_tag="VNN25_24600"
     CDS             complement(238..1215)
                     /locus_tag="VNN25_24600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH94777.1"
                     /translation="MNKRSLGSQELVVSAEGLGCMGMSQSYGPADEQESLATIDRAIE
                     LGIDFFDTAEVYGPYKNEELLGRALRGRRDRVIVATKFGWKIEDGKMTGVDSSRIREA
                     VDGSLRRLGTDHIDLLYQHRVDPKIPIEDVVRTMSDLVREGKVRFLGLSEAGEQTIRR
                     AHAVHPITALQSEYSLWERNLEPRIIPLLRELGIGLVPFSPLGRGFLAGSAKRAEDYP
                     EGDFRRNDPRFQGENFDANVRAASIVRDIATSRNATPAQIALAWLLHKGDDIVPIPGT
                     KRRKYLEENVAAASITLTADEMKRLDESLRPEAISGPRYNERAMGWVDR"
     gene            1342..1638
                     /locus_tag="VNN25_24605"
     CDS             1342..1638
                     /locus_tag="VNN25_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94778.1"
                     /translation="MKRTMVLFAALVLVAATGCMEKKTESTSTVSGTDTVSSSTTTIS
                     TTVPAVDTATTAAAKADVKELGKDAAEAGREAAHGTGTAMETAGKEIQRKTKKN"
     gene            complement(1672..2415)
                     /locus_tag="VNN25_24610"
     CDS             complement(1672..2415)
                     /locus_tag="VNN25_24610"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXH94779.1"
                     /translation="MTIKRLLPFLALLLLASPAGASIALFVDGRAMKISSYKVVSESE
                     VQLSLTSGGAITVPLTRVDRILDDEIVPAAIVEEVKKAVEKDGGIFPTRSWRYDEARK
                     PIFKSKFDAIIVEAAKKFDVDAALVSAVIKAESDFNAREFSDKGARGLMQLMPSTAER
                     FGVTDSFDPAANIYGGVRYLHWLLKTFNGNADLAVAAYNAGEGNVWKYNGVPPFRETI
                     NYINRIAKHIRNAMPAAPAANKVADAHAR"
     gene            complement(2469..3404)
                     /gene="xerD"
                     /locus_tag="VNN25_24615"
     CDS             complement(2469..3404)
                     /gene="xerD"
                     /locus_tag="VNN25_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXH94780.1"
                     /translation="MRKSPRAADPDKKETGPYFLTWLPIFLDYLAVEKGLAKNSLSAY
                     QSDLKHFGHWLTDQGMDLEQVKRIQIVRYFQALRTAGISARSVARALAAIRGLFRFLV
                     SEKHLKHDPTENIENPKLWTNLPKSMPPEEVEALLRAPDRNTPDGLRDAAMLELLYAT
                     GLRVSELIHVKVEDLVMDAGFLRTIGKGSKERIVPFGDSARDTIVAYLERGRRGFEKH
                     ADPHLFLSNRGRPMSRQAFWMKIVRYARQAGIAGHISPHVLRHSFATHLLENGADLRS
                     VQMMLGHSDISTTQIYTHVSRARLQKMYEQFHPRA"
     gene            complement(3397..4263)
                     /gene="lipA"
                     /locus_tag="VNN25_24620"
     CDS             complement(3397..4263)
                     /gene="lipA"
                     /locus_tag="VNN25_24620"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXH94781.1"
                     /translation="MSARLPDWIREKKINLRELHEVKSLLREKSLHSVCESLACPNRS
                     ECFSRGTATFMILGDVCTRSCGFCNVTTGRPYAPPAADEPRSVAAAAKKMSLRHVVVT
                     CVTRDDLDDGGARQFALTIQALRDALPDAAVEVLTSDFRGNFESVRTVVEAKPDYFNH
                     NVETVPRLYDFVRPGSRFERSLAILREAKRIDPGVTTKSGLMLGLGERRDEVVDVLQR
                     LNESHVEIVTIGQYLQPKREKLDVVEYIAPRVFDEYRDIGEAMGFRAVFAGPFVRSSY
                     MADAVAHEAANA"
     gene            complement(4453..4953)
                     /locus_tag="VNN25_24625"
     CDS             complement(4453..4953)
                     /locus_tag="VNN25_24625"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXH94782.1"
                     /translation="MKLGFKVGEKLVYPNHGVTIVEQIGSSPIMGADDTYYHLRLLAN
                     NSRLMVPITNTDRVGLRPLYKQTQIKGLLSLLEETTQKAHTDWKGRYRENLEKMKTGR
                     LEDVAEVLKNLSEVQKKKSLSFREKKMYDRAKYLLVSEVAIVKNIPEIEAEKLIARSL
                     EKSLGH"
     gene            complement(5115..6044)
                     /locus_tag="VNN25_24630"
     CDS             complement(5115..6044)
                     /locus_tag="VNN25_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94783.1"
                     /translation="MKSVIAVAVILTATAGLAQVPAARVAADARVIDRVAEASRNDLP
                     RDLLTRIVNEDIDFLRGKRADGTYQYASYDRMEAGRKSDTISVDPERKESVLELRDAF
                     AFRLIISVPSRRMLVTKNHRIYIDHVEIESLPQNSSEKKFQTVKLGAWIEPGSAQTID
                     LDDIGRQAIARVYAHAEGSGYANVTLTLVEARIFDEPSSPYADAVESEKAILNGLKHD
                     DKPSIRAMAQRISSRLQPVSAAGTAPMSSPPATIAAIPATSQMEVISGRTDPETLAEL
                     QAIEDLLTGSEAERRQGLDRLHQLVRKLRSPSR"
     gene            complement(6041..7210)
                     /locus_tag="VNN25_24635"
     CDS             complement(6041..7210)
                     /locus_tag="VNN25_24635"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HXH94784.1"
                     /translation="MTESRAIVIVCDSLGVGELPDAKDFGDEGSNTLGHVLESQHPNL
                     PTLSRLGLLHTLPKPATSETPRAAFGRMAEVSAGKDTTTGHWEMMGLIVEDPFRTYPN
                     GFPADVIAEYERRIGRKTLGNKAASGTVILDELGEEHVRTGSPIVYTSADSVFQVAAH
                     EDVIPVEELWRICAVARELMRGEHNIGRIIARPFIGSGKGHFKRTANRKDFSVRPTGL
                     TVVERAFNSGKQVIGLGKIADIFDRVGISQEIRTENNSDGMRKTIDLIRQSDADFIFT
                     NLVDFDSKYGHRNDAPGYAKALETFDEELAALLGDLRRDDLLFITADHGCDPTDISTD
                     HTREYVPLIVAGPRVAATSLGTRSSFADLGATICEHLGISSDGIPGRGVLKEVLQ"
BASE COUNT         1293 a         2113 c         2454 g         1503 t
ORIGIN      
        1 gcacatcgaa ggcacgttca tcggatttga agtgccggcc gggacctcgg acgttcgcgt
       61 cgtttatcgg ccgatgagct tctatgggtc gtgcgcggcg gcgctgctga ctgtgttggt
      121 gctgtggttt tggcctgtgc ggaaagttgc cgccgagccg gcggcggaac agccgtcgag
      181 ccgacggcgc tacgcttgac cgttgtcaat tgtcaattat caattgtcca ttaacgccta
      241 ccggtcgacc caccccattg ctcgctcgtt gtatctcggg ccggagatcg cctcggggcg
      301 cagggactcg tcgaggcgtt tcatctcgtc tgcggtcagc gtgatcgatg cggcggcgac
      361 attctcttcg aggtacttgc gccgtttcgt gccggggatc gggacgatgt cgtcgccttt
      421 gtgcagcagc caggcgagtg cgatttgcgc gggggtcgcg ttgcggctcg tggcgatgtc
      481 gcgcacgatc gaggcggcgc gcacgttcgc atcgaagttc tcgccctgaa accgcggatc
      541 gttccggcgg aagtcacctt ccggataatc ctcggcacgc ttcgcactcc ctgcgagaaa
      601 tccgcgcccg agggggctga agggaacgag gccgatgccg agctcgcgca gcagcggaat
      661 gatgcgcggc tcgagattgc gctcccacag cgagtactcg ctctgcagtg cggtgatcgg
      721 atgaacggcg tgcgcgcggc ggatcgtctg ctcgcccgct tcggaaaggc cgaggaatcg
      781 gacttttcct tcgcgaacga gatcgctcat cgtgcgtacg acgtcctcga tcggaatctt
      841 cggatcgacg cggtgctggt agagcagatc aatgtgatcg gtccccaagc ggcgcagcga
      901 tccgtcgacc gcttcgcgaa tgcggctgct gtcgacgccg gtcatcttgc cgtcctcgat
      961 cttccatccg aacttcgtag cgacgatgac gcgatcccgc cgccctcgca gcgcgcgccc
     1021 gaggagctcc tcattcttgt acggcccata cacctcggcc gtgtcgaaga agtcgatgcc
     1081 aagctcgatg gcgcgatcga tggtggcgag cgattcctgc tcatcggcag gcccgtacga
     1141 ttggctcatc cccatgcacc cgaggccctc ggcggagacg accagctcct gagatccaag
     1201 cgagcgtttg ttcatggtgt aacgctacta cgggcatcgc ttgatcggga agacaatggc
     1261 gcactcttgg cgcctttgcc ttcgcatgaa atcgcggcga tggctgcgtt cttgccatgt
     1321 gattccggca ggagatcaac catgaaaaga acgatggtgt tattcgcggc cctggttctc
     1381 gtcgcggcga cgggatgcat ggagaagaaa acggaatcga cgtcgacggt gagcgggacc
     1441 gatacggtgt cgagcagcac gacgacgatc tcgacgaccg ttccggcagt cgacacagct
     1501 acgacagcgg cggcgaaggc cgatgtcaag gaactgggga aggatgcagc ggaggccggg
     1561 cgggaggcag ctcatggcac cggcaccgcg atggaaacgg ccgggaaaga gattcagcgc
     1621 aagacgaaaa agaactagcc gcgcgggttg acgcgctacg tgcgatcgcg cctaacgtgc
     1681 gtgcgcgtcc gcgactttgt tcgcggccgg cgccgcgggc atggcattgc ggatgtgctt
     1741 cgcgatccgg ttgatgtagt tgatcgtctc gcggaatggc ggcacgccgt tgtacttcca
     1801 gacgtttcct tcgccggcgt tgtaggcggc gacggcgagg tcggcgttgc cgttgaacgt
     1861 cttgagcaac cagtgcaggt agcgcacgcc gccgtaaatg ttggcggccg gatcgaagga
     1921 gtcggtcaca ccgaagcgct ccgcggtcga gggcatgagc tgcatcagac cgcgggcacc
     1981 tttgtccgag aactcccgtg cgttgaagtc ggactccgct ttgatcaccg cggagacgag
     2041 cgcggcatcg acgtcgaact tcttcgccgc ttcaacgatg atggcgtcga acttcgactt
     2101 gaaaatcggc ttgcgggctt cgtcatagcg ccaggagcgg gtcgggaaga tgccgccgtc
     2161 tttttcgacc gctttcttca cctcttccac gatggcggcc gggacgatct catcgtcgag
     2221 gatgcggtcg acgcgcgtga gcggaaccgt gatggcgccg ccgctcgtga gcgagagctg
     2281 gacctcactc tcgctgacga ctttgtagct gctgatcttc atcgcccgcc cgtcgacgaa
     2341 gagggcaatg ctcgcgcctg ctggcgaggc gagcagaagg agcgcgagga atggtagaag
     2401 ccgcttgatc gtcatcgtgg gcaacgctgt gaactaggac gttgcgaatg ttattgcagt
     2461 tccatccctc atgcacgcgg atggaattgc tcgtacatct tctggagccg ggcccgggac
     2521 acgtgcgtgt agatctgcgt cgtcgaaata tcgctgtgac ccagcatcat ctgcacgctg
     2581 cggaggtcgg cgccgttctc caaaaggtgc gtggcgaagg agtggcgcag gacgtgcggg
     2641 gagatgtgcc cggcgattcc tgcctgccgc gcgtaacgaa cgatcttcat ccagaaggcc
     2701 tgccgtgaca tcggccggcc gcggttggag aggaagagat gcggatcggc gtgcttttcg
     2761 aagccgcgcc gtccccgttc gagataggcg acgatcgtgt cgcgggcgct gtcgccgaag
     2821 gggacgatcc gctctttcga ccccttgccg atcgtgcgca ggaagccggc gtccatcacc
     2881 agatcctcga ctttcacgtg gatgagctcg gagacgcgca ggcccgttgc gtagagcagc
     2941 tcgagcatcg ccgcgtcgcg taggccgtcg ggggtgttgc ggtcgggtgc gcgcaggagc
     3001 gcttccactt cctcgggcgg catcgacttc ggcaagttcg tccagagctt cgggttctcg
     3061 atgttttcgg tcggatcgtg cttcagatgt ttctcggaca cgagaaaccg gaacagtcct
     3121 cgtatcgccg caagggcgcg tgccacggag cgggcggaaa tgcctgccgt gcgcagggcc
     3181 tggaagtagc ggacgatctg aatccgcttc acctgctcga gatccatccc ctgatccgtg
     3241 agccaatgtc cgaagtgttt gaggtctgac tggtacgcgg acagcgagtt tttcgccagc
     3301 cctttttcga ccgcgaggta gtcgagaaaa atcggcagcc aggtcagaaa gtacgggccg
     3361 gtctcttttt tgtccgggtc ggccgcgcgc gggctcttac gcattggctg cttcatgcgc
     3421 caccgcatca gccatgtagg agctgcgcac gaaaggacca gcgaaaacgg cgcggaaccc
     3481 catcgcttcg ccgatgtcgc gatattcgtc gaagacgcgc ggagcgatgt actcgacgac
     3541 gtcgagtttt tcgcgcttcg gctggaggta ctggccgatg gtgacgatct cgacgtgcga
     3601 ctcgttcagg cgctgcagca cgtcgacgac ttcgtcgcgc cgctcgccga gcccgagcat
     3661 cagacccgac ttggtggtga cgccgggatc gatccgtttc gcttcccgca ggattgccag
     3721 cgagcgctcg aaacgcgagc ccggacggac gaagtcgtag agccgcggaa cggtctcgac
     3781 gttgtgattg aagtaatcgg gcttcgcttc gacgacggtc cgcacggatt cgaagttacc
     3841 gcggaaatcg gaggtgagca cctcaacggc ggcatcgggc agggcatcgc gcagcgcctg
     3901 aatcgtgagt gcaaattgcc gcgcgccacc atcgtcgaga tcgtcacgcg tgacgcaggt
     3961 gaccacgacg tgtcgcaggc tcattttctt tgcggccgcg gcaaccgagc gcggctcatc
     4021 ggctgcgggc ggagcgtagg gacggccggt cgtgacgttg cagaagccgc aggagcgcgt
     4081 gcagacgtcg ccgaggatca tgaaggtggc ggtgccgcgc gaaaagcact cgctgcggtt
     4141 gggacaggcc agcgactcgc agacggagtg cagcgacttc tcgcgcagca gcgatttgac
     4201 ctcgtgcagc tcgcggaggt tgatcttctt ttcgcggatc cagtcgggga ggcgggcgga
     4261 catcgggtta catgctagca aaggggggtg gggagtgggg ggtagggagt ggtatccgca
     4321 gatgacgcag atgtgcgcag atgaaagacc gaagcgccaa gtcgatctgc gtcccatctg
     4381 cgtcatctgt ggatcaaaac aaaaccgccg cccgtttcgg ggcggcggct gattggactt
     4441 cagtgctgtg agttaatgac cgagcgactt ctcgagcgac cgggcgatca gcttctccgc
     4501 ctcgatctcg gggatgttct tcacgatcgc gacttccgac acgagcaggt atttcgcgcg
     4561 gtcgtacatc ttcttctcgc ggaacgagag gctcttcttt ttctgcactt cgctgaggtt
     4621 cttgaggact tcggcgacgt cctcgagacg gccggtcttc atcttctcga ggttctcgcg
     4681 gtaccggcct ttccagtcgg tatgcgcttt ctgcgtcgtc tcttcgagga ggctgagaag
     4741 gcctttgatc tgtgtttgct tgtacagtgg gcgcaggccg acgcggtcgg tgttcgtgat
     4801 cgggaccatc aggcgcgagt tattcgcgag cagcctgagg tggtagtagg tgtcatccgc
     4861 acccatgatc ggcgaagaac cgatctgttc gacgatcgtg acgccgtggt tcggatagac
     4921 aagtttttcc ccgactttga aacccaattt cattgaagac aagtagaact cctttcggaa
     4981 gactggtgaa gcttgaggga tcggtattct acgtcatcgt gaccgtatcg tcaatcaggt
     5041 gaggcgctta atgtgagcgg gggaagctca accgttgacg atttaccgac gattcggctt
     5101 cttgtaacgg ccgttcaacg cgatggagac cgaagcttgc ggacgagctg gtgaaggcgg
     5161 tcaagtcctt gacgccgctc agcttcgctc ccggtgagga ggtcctcgat cgcctgcagc
     5221 tcggcgagcg tttcggggtc ggtacggccc gagatgacct ccatttggga cgttgctgga
     5281 atcgcggcaa ttgttgccgg aggactgctc atcggagcgg ttccggcggc tgagaccggc
     5341 tgcaatcggg aggaaattcg ctgggccatc gcccggatgg agggtttgtc gtcgtgtttc
     5401 agcccattca ggatcgcctt ttcgctctcc acggcatctg cgtaggggct ggacggctcg
     5461 tcgaagatgc gggcctctac gagggtgaga gtcacgttgg cgtagccgct gccctccgcg
     5521 tgcgcatata cccgtgcaat cgcctggcgg ccgatgtcgt cgagatcgat ggtctgtgcg
     5581 gaaccgggct cgatccacgc gccgagtttc acggtctgga acttcttctc cgaggagttt
     5641 tgcgggagcg attcgatctc gacgtggtcg atgtagatgc gatggttctt cgtgaccagc
     5701 atgcgccgcg acggaacgct gatgatgagc cggaaggcaa aggcgtctcg cagctcgagc
     5761 accgactcct tgcgctcggg atcgaccgag atcgtgtccg acttgcggcc ggcctccatc
     5821 cggtcgtagc tggcgtattg ataggtgccg tcggcgcgct tgccgcggag aaaatcgatg
     5881 tcttcgttga cgatgcgggt cagcagatcg cgaggaagat cattccggga tgcttcggcg
     5941 acacggtcga tcactctcgc gtcggcggcg acgcgggccg ccggtacctg cgcgaggccg
     6001 gcggttgctg tgaggattac ggcaacagcg atgacgctct tcattgcagc acctccttca
     6061 aaactcctcg gcccggaatc ccgtcgctgg agatgccgag gtgctcgcag atggttgcgc
     6121 cgaggtcggc gaacgatgat cgggtgccca gcgaagttgc cgcgacgcgc ggcccggcca
     6181 cgatgagcgg cacgtactcg cgggtatggt ccgttgagat gtcggtggga tcgcagccgt
     6241 gatcggcggt gatgaagagc agatcgtcgc ggcgcaggtc gccgagcagt gcggccagct
     6301 cctcatcgaa cgtctccagc gctttggcgt agccgggcgc gtcgttgcgg tggccatact
     6361 tcgaatcgaa gtcgacgagg ttcgtgaaga tgaaatcggc gtccgactgc cggatgagat
     6421 cgatggtctt gcgcatgccg tcggagttgt tctcggtacg aatttcctgg ctgatgccga
     6481 cgcggtcgaa gatgtcggcg atcttgccga gtccgatgac ctgcttgccg gagttgaacg
     6541 cgcgctcaac cactgttaag ccggtcggcc gcacggaaaa gtctttccga ttggccgtgc
     6601 gcttgaaatg ccccttgccg ctgccgatga acggacgggc gatgatgcgg ccgatgttgt
     6661 gctccccgcg catgagctcg cgggcgacgg cacagatgcg ccacagctct tcaacgggga
     6721 tgacgtcttc gtgcgcagcc acctggaaca cggaatcggc ggaggtatag acgatcggac
     6781 tgccggtgcg aacgtgttcc tcgccgagct cgtcgaggat cacggtaccc gaggcggctt
     6841 tgttgccgag ggtcttgcgg ccgatgcgcc gctcatattc ggcgatgaca tcggcaggga
     6901 agccattcgg atacgtgcgg aaagggtcct cgacgatgag ccccatcatc tcccagtgac
     6961 ctgtagtcgt gtccttcccc gcgctgactt ccgccatgcg cccgaacgcc gcgcgaggtg
     7021 tctcgctggt tgcaggcttg ggcagcgtgt gcagcaggcc gaggcgggaa agcgtgggca
     7081 ggttcggatg ctgtgactcg agcacgtgcc cgagcgtgtt ggaaccttcg tcgccgaagt
     7141 ctttggcatc cggaagctcg ccgaccccga gggaatcgca gacgatgacg atggcacggc
     7201 tctctgtcat agtctgatta ttacctgagg tgacaggcat atgcacatcg cggtgaagtg
     7261 ggttaccttc ctggcggcgg cttccctctt tttctatttt gcaaaatacg agttcggtcc
     7321 tcggaagtat ccaacgaaga cactggacgg ccgctgcttc tac
//
LOCUS       DATLVW010000841         6547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_34181, whole genome shotgun sequence.
ACCESSION   DATLVW010000841 DATLVW010000000
VERSION     DATLVW010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34181"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..688
                     /locus_tag="VNN25_24640"
     CDS             29..688
                     /locus_tag="VNN25_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94785.1"
                     /translation="MTDQPGNPNNPNDPTTPPPDVSPDELAAKLTALLDQFESQVPGF
                     QPHNSAEIRRVSNAARYGKELIPLMITAVTSFPPAAERKIFDVERGKATLLAEDALRP
                     VAHRLSALLDGFAFTLDSNLGGTSTEALHGYAWFKHFAKSPEGVGVRPFLGNMKSAVR
                     KIINRKPVKKPAPPTIPPGAQGLLAPNLGNRNTTPEVIEDDLPEDFRKALEAVSDDNE
                     E"
     gene            785..991
                     /locus_tag="VNN25_24645"
     CDS             785..991
                     /locus_tag="VNN25_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HXH94786.1"
                     /translation="MNEIIFLVEEAPEGGFTARALGQSIFTEADDFASLEDNVRDAVR
                     CHFEANEMPHVVRLHFTREQVIAV"
     gene            988..1233
                     /locus_tag="VNN25_24650"
     CDS             988..1233
                     /locus_tag="VNN25_24650"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXH94787.1"
                     /translation="MRLPRDWSGDELVRRLARVGYSVTRQTGSHVRLTKPPPPSEHHV
                     TVPRHDTLRIGTLAGILDDVAAHLEITRDELLARLAA"
     gene            1483..2907
                     /locus_tag="VNN25_24655"
     CDS             1483..2907
                     /locus_tag="VNN25_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94788.1"
                     /translation="MDTEQILREMKLFSLGGEGIGGWITPDSDSRIFERLGRLDLEPL
                     SAVQLNQLLVFGKESPVSDDFFRYYWLEDPLDHPYPVGLLPGFSKKWVQHNSMILSLE
                     HLKWGLHRLFVDSLLYFGNVRTAYRRLRSLTKSELIEFFASRRFDTSRIKERGPALPL
                     SPIPKDDRYLISEMACKSYGDDPSGSGDLKHFLAAALGQHHKAGGGKIKLRDLLQMKP
                     PTYGQRQSEFEFSATELLDEEIFTSDDLDAKLVPLVARFFKARDAALENTRYYLSMAD
                     DLDVYVATSMRGREDFRKMASTCETIFADSRLKELQLRYFDPTLSAAAGHEDKGIIEC
                     LMVKCAKLLVYCAGEKESYGKDAEAAMALSLGKPVIFYCDEDQRSRFYRDVHPLSRLV
                     EFQTGVVVGAMVTDTQGQVSELLYRIFENKMEYVLEQPKPGHLRLKDRLTDSVVRVQT
                     GDPMLSATFYNLYHNRDAAIRRGA"
     gene            3006..3773
                     /locus_tag="VNN25_24660"
     CDS             3006..3773
                     /locus_tag="VNN25_24660"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXH94789.1"
                     /translation="MITTKSPATWRDLQNAVARILSECGFIVEVEKRITTARGRVEVD
                     VYAQEIVQGRSYEILCECKHWKRAVPQTAIHSFRTVIAEIGANVGYFISLHGFQAGAF
                     AASELTNIQLVTWNQFQEAFEETWRERYFSPMIEERLDPLLTYTEPLAPDWFVKLPES
                     EKAEFIHLKERYDPFGFIMMSLTSYFRIFNKEPLPKLPLEEHIDPRFKEAIPHELLIA
                     TGYREFFDLAIRYGDIAISEFRAIRDRNGLANDSPHE"
     gene            4113..>6547
                     /locus_tag="VNN25_24665"
     CDS             4113..>6547
                     /locus_tag="VNN25_24665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH94790.1"
                     /translation="MKLGIEDFEYSHLYDNRRLQDLALAFDRFLERSDAGLLQRFESY
                     RVGVQSGIAGGGLTKPEESALLIAVSRPLGTFLEQLFQTDSASVIARTQRDAEVARFK
                     KEFVTKRVAKVQDIAAVDSQRAIDILVRTIAPGHEHGQEHDLEFAIAVTANRLLDFER
                     EYPRGTKTPAPSPQTRAALGQLRDELRTSGAFAEAIVYKQSVESPEAIAREAAAVHAL
                     GDLLVDWLAAEWKAGRFDEWTSFRLPKPMNFDHLVETERVDENTVVGPSDKYRRRDGF
                     KLTDARMTSREITDEANYCIYCHERKKDSCKTGLAEKDERVKINPLGVPLAGCPLDER
                     IGEMNALRKDGDSIAALAIVMIDNPMCPGTGHRICNDCMKSCIFQKQDPVNIPQIETG
                     ALTDVLWLPWGFEIYSLLTRWNPLNVRRPMALPYNGKNVLIVGLGPAGYTLAHYLANE
                     GFGVVAIDALKIEPIDQQWLRQPIRDAHTTLWEELDERILVGFGGVSEYGITVRWDKN
                     FLKVIRIALERKNNIRVYGGVRFGGTLTIEDAFDKLGFDHIAIAAGAGTPTIVRMKNN
                     LIRGIRKASDFLMALQLTGAGKQSAMANLQVRLPALVIGGGLTAIDTATELFAYYPLQ
                     VQKTLSRYETLSSEIGETTVRSGYDKEELIILDEFLEHGRAIRAERERAASVGEKPNF
                     IPLVRSWGGVTIAYRKSIGDAPAYRLNHEEVIKAFEEGIAYAESLSPVEAIADEFGHV
                     KSLLFEKQINEDGRWKDSGNIIELAARSVMVAAGTSPNVIYEKEHPGTFKLDKYGQFF
                     QSFAVSAADAGPEL"
BASE COUNT         1444 a         1967 c         1870 g         1266 t
ORIGIN      
        1 cgttcaaccg tagtctcgga ggtaccccat gaccgatcaa ccaggaaacc ccaacaaccc
       61 taacgacccg accacaccgc cacccgatgt ctccccggac gaactggccg ccaaactgac
      121 cgcgcttctc gatcagttcg aaagccaggt tccgggattc caacctcaca acagcgctga
      181 gattcgtcgc gtgtccaacg ctgcccgcta cggcaaggag ctgatcccgc tgatgatcac
      241 cgccgtgacg tcgttccccc ccgccgcgga gcggaagata ttcgacgtcg aacgcggaaa
      301 ggcgacgctt ctggccgaag acgccctccg ccccgtcgca caccgtctct ccgccctcct
      361 cgacggcttc gcattcacgc tcgacagtaa cctcggcggc accagcaccg aagctctcca
      421 cggctacgca tggttcaagc attttgcgaa gagccccgag ggcgtcggtg tccgtccatt
      481 cctgggcaac atgaagagcg cggtccgcaa gatcatcaac cgcaaacctg tgaagaagcc
      541 cgccccaccc acgattccgc ccggcgccca gggactcctc gctccaaacc tcggcaaccg
      601 caacaccacc cccgaagtca tcgaagacga tctcccggag gatttccgca aagccctcga
      661 agccgtcagc gacgacaacg aggagtaagc ctcaactctt aagtgcattc cgatcgagcg
      721 ccttctcccc atcgcttttt gatggggaga aggtgcttcc ggagaagtca gtacaatgat
      781 gtgcatgaac gagatcatct ttctcgtcga ggaagctccc gaaggcggat tcacggcccg
      841 agcgcttggc caatcgatct tcaccgaagc ggacgacttc gcctctctcg aagacaacgt
      901 gcgcgatgcc gtgcgttgcc acttcgaggc gaatgagatg ccacacgtcg tacggctcca
      961 tttcacgcgc gaacaggtca tcgcggtttg agactgccgc gcgattggtc aggagatgag
     1021 ctagtccgca ggctggctcg cgtcggctat tcagtaacgc gacaaaccgg aagccacgtg
     1081 cgcctgacca agccaccacc accaagcgag caccacgtca cagtccctcg gcacgacacg
     1141 ctgcgcatcg gcacgctcgc agggatcctg gacgacgtcg cggcccatct cgagatcaca
     1201 cgcgatgagc tgctcgcgcg tctcgcggct tgagcgggga tcgatgcggt gcctccgcgc
     1261 ccgtcgagcc aaccgtcaag gaaatctcgg ccatccgtcg ccacaagcgg ctgctgctgc
     1321 gacccaggcg cccgcgcaat cgtcgcagtc acgaccgaaa cctcgaaaga ggaaccaatt
     1381 gttagcggtc tagcatgaca atagttggag gccgcaaaat ttgcatattc atgcacaccc
     1441 agagtagtct tatccgactt ttttactgaa cgaggccatt gagtggacac tgaacaaatt
     1501 cttcgggaaa tgaagttgtt ctcgctgggc ggcgaaggaa tcggaggttg gatcactcca
     1561 gattccgact ctcgcatttt tgagcgcctc ggacgcttgg accttgaacc cctaagcgcg
     1621 gtacagctca atcaattgct ggtcttcggc aaagaatcac ccgtgtccga cgatttcttt
     1681 aggtattact ggctcgaaga tccgctcgac catccatatc cagttgggtt gcttcctggc
     1741 ttcagcaaga aatgggttca acacaactcg atgattcttt ctcttgagca tcttaaatgg
     1801 gggctgcatc ggctgttcgt tgatagcctc ctctactttg gaaacgtccg gaccgcctat
     1861 cggcggttac gaagcctaac gaagagcgag ttgatcgagt tcttcgcaag tcggcggttc
     1921 gacaccagtc gaattaaaga acgcgggccg gctctccctc taagcccgat cccaaaagac
     1981 gaccgctatc tgatctcgga aatggcttgc aagtcgtacg gcgacgatcc ttctggatcc
     2041 ggcgatctaa aacactttct agccgccgcg ctaggtcagc atcacaaggc tggcgggggc
     2101 aaaatcaagc ttagagattt gttgcagatg aagccaccaa catacggtca acgccaaagc
     2161 gaatttgagt tttccgcgac agaactgctc gacgaagaaa tctttacgtc tgacgacttg
     2221 gacgctaaac ttgtcccact agtcgcgcgc ttctttaagg cacgggacgc agcgctagag
     2281 aacacccgat attacttgtc gatggccgac gacttggacg tatacgtcgc tacaagtatg
     2341 cgcggccgcg aagatttccg gaagatggcg agtacttgcg aaaccatctt cgctgattct
     2401 cgcctaaaag aattgcagtt gcgatacttt gacccaacat taagcgccgc agcaggtcac
     2461 gaggacaaag gcattatcga atgtctgatg gtaaagtgcg ccaaacttct agtttattgc
     2521 gccggtgaga aagagagtta cggcaaggat gcggaagctg caatggcact gagtctcggc
     2581 aagccagtga tattctattg tgacgaagat cagcgtagtc gcttctaccg tgacgttcat
     2641 ccgctcagcc ggcttgtgga gtttcaaaca ggtgttgtag taggtgcaat ggttacggac
     2701 actcagggac aggtatccga gttgctatac cggatcttcg agaataagat ggagtacgtt
     2761 ctagaacaac caaagcccgg tcatcttcga ctaaaagaca ggctgaccga ctcagtggtc
     2821 cgcgttcaaa ctggggaccc gatgctgagt gccacattct acaatctgta ccacaatcga
     2881 gatgctgcga tacgccgcgg tgcgtagctt ccggttaatg atccacactt gatgaccgga
     2941 aatcatcgcc gcgccagcaa acaaggcaga tgggatggcc ggtatgagcg ccgccaggac
     3001 caatcatgat cacgacaaag agtcccgcca cttggcgcga tcttcaaaac gccgtcgcac
     3061 gcattctctc cgagtgcgga tttattgtgg aggtagaaaa gcgaattacg accgcgcgag
     3121 gcagggttga agtcgatgtc tacgcgcaag agattgtgca agggcgtagc tatgagattc
     3181 tctgcgaatg caagcactgg aaaagggccg tacctcagac ggcgattcac tcgtttagaa
     3241 ctgtcatcgc agagatcggg gcgaatgttg gctacttcat ttctttgcac ggctttcagg
     3301 cgggggcgtt tgccgcatcg gagctgacga atattcagct tgttacttgg aaccagtttc
     3361 aggaggcatt tgaggaaact tggcgagaga gatacttctc tccaatgatt gaagaacgac
     3421 tcgatccgct cctgacctac acggagccgc tagcgccaga ctggtttgtt aaacttcccg
     3481 aatcggaaaa ggctgaattc attcacttga aagaaaggta cgatcccttt ggctttatca
     3541 tgatgagcct cacgtcttac ttccgtatct tcaacaagga gcccctcccg aaactgccgc
     3601 ttgaagagca cattgatccc cggttcaagg aggcgatccc ccatgagcta ctcatcgcga
     3661 cgggataccg tgagtttttt gacctggcca tccggtacgg cgatatagcg atctccgagt
     3721 tccgcgcgat cagagaccga aatggacttg cgaacgactc tccgcacgaa tgagcgaggt
     3781 ggcgacttta cccggtgacg gtggtcgggt ggctagtgcc aaattcggaa ggttcagatt
     3841 gagttcctag agtagcgcca cgatgccgcc tacatcgagc ttcccagcgg acggaagctg
     3901 atgacctaat ggatgctgag cagttcggcg tggcgcgaaa tctcggtgtc cgccgtcaca
     3961 tcgcttcgtg accctcctcc tccgaggcgc gcccgtcccg ctgctaccgt tcaccttgtt
     4021 ttcagggacg ggatcattcg accgtccttc ggcacttcgt gtccttccac ggctgaggat
     4081 gacagtgaag atttttggtg ttggagcgaa ttgtgaaact aggaattgaa gacttcgagt
     4141 actcgcatct gtacgacaac cgccgtttgc aggatctggc gctggcgttt gaccggtttc
     4201 tggaacggag cgacgcgggc ctgttgcagc gcttcgagtc gtatcgcgtc ggcgtgcaga
     4261 gcgggatcgc cggcgggggg ctgaccaagc cggaagagtc ggcgctgctg atcgcggtca
     4321 gccggccgct ggggacgttt ctggagcagc tcttccagac ggattcggct tcggtcatcg
     4381 cgcgcacgca gcgggatgcc gaggtggcgc ggttcaagaa agagttcgtg accaagaggg
     4441 tggcgaaggt tcaggacatc gcggccgtcg attcgcagcg tgccatcgac atcctcgtac
     4501 gcaccatcgc ccccggacac gagcatggtc aggagcacga cctcgagttc gcgatcgccg
     4561 tcaccgccaa ccgcctgctc gacttcgagc gcgagtaccc gcgcggcacg aagacgcctg
     4621 cgccttcgcc gcagacgcgc gccgcgctcg ggcagttgcg cgacgagctg cggacatccg
     4681 gcgcgttcgc ggaagccatc gtttacaaac agtcggtcga gtctcccgag gcgattgcaa
     4741 gggaagcggc ggccgtgcat gccctcggcg atctcctcgt cgactggctg gcagcggagt
     4801 ggaaggccgg acgcttcgac gagtggacat cgttccgttt gccgaagccg atgaacttcg
     4861 atcatctcgt cgagacggag cgtgtcgacg aaaacaccgt ggttggcccg agcgacaaat
     4921 accgccgccg cgacggattc aagctcaccg atgcgcgcat gacctcgcgc gagatcaccg
     4981 acgaagccaa ctactgcatc tattgccacg agcggaagaa agactcctgc aagacgggtc
     5041 tcgccgaaaa ggacgaacgc gtcaagatca atcccctcgg cgtgccgctc gcgggctgcc
     5101 cgctcgacga gcgcatcggc gagatgaacg cgctgcgcaa agacggcgac tccatcgccg
     5161 cgctggccat cgtcatgatc gacaacccga tgtgccccgg caccggccac cgcatctgca
     5221 acgactgcat gaagtcgtgc atcttccaga agcaggaccc ggtgaacatc ccgcagatcg
     5281 agaccggcgc gctgacggac gtgctctggc tgccgtgggg cttcgagatc tattcgctgc
     5341 tgacgcgctg gaatccgctg aacgtgcggc ggccgatggc gctcccctac aacggcaaga
     5401 acgttctaat cgtcggtcta ggacctgccg gctatacgct ggcgcactac ctcgccaacg
     5461 aaggcttcgg agtcgtggcc atcgatgcgc tgaagatcga gccaatcgat caacagtggc
     5521 tgcgtcagcc gatccgcgat gctcacacga cgttgtggga ggagctcgac gagcgcatcc
     5581 tggtgggctt cggcggcgtg tcggagtacg gcatcaccgt ccgctgggac aagaacttcc
     5641 tcaaagtcat ccgcatcgcc ctggagcgca agaacaacat ccgcgtctac ggcggcgtgc
     5701 gcttcggcgg gacgctgacg atcgaagacg ccttcgacaa acttggattc gatcacatcg
     5761 ccatcgccgc cggcgccgga actccgacca tcgtgcggat gaagaacaac ctcattcgcg
     5821 gcatccgcaa agcgagcgat ttcctgatgg cgctgcagct caccggcgcc ggcaaacagt
     5881 cggccatggc caacctgcag gtgcgcctgc cggcgctggt gatcggcgga ggcctgaccg
     5941 cgatcgacac ggcgacggag cttttcgcct actacccgct gcaggtgcag aagaccctca
     6001 gccgctacga gacgctctct tccgagatcg gcgagacgac cgtccgcagc ggttacgaca
     6061 aagaggaact gatcatcctc gatgagtttc tcgaacacgg tcgcgccatc cgcgccgagc
     6121 gtgagcgggc tgcgtcggtt ggcgagaagc ccaacttcat cccgctcgtc cgctcctggg
     6181 gcggcgtgac gatcgcctat cgcaaatcga tcggcgacgc gccggcctac cgtctcaatc
     6241 acgaagaggt gatcaaagca ttcgaagaag ggatcgctta cgccgagagc ctctcgccgg
     6301 tcgaagcgat cgccgacgaa ttcggccacg tgaaatcgct cctcttcgaa aaacagatca
     6361 acgaagacgg ccgctggaaa gactccggaa acataatcga gcttgccgcg cgcagcgtga
     6421 tggtggccgc gggaacgagc ccgaacgtga tttacgagaa ggagcatccg ggcacgttca
     6481 agctcgacaa gtacggacag tttttccaga gcttcgcggt ctcggcggcc gatgccggcc
     6541 ccgagct
//
LOCUS       DATLVW010000842         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_34268, whole genome shotgun sequence.
ACCESSION   DATLVW010000842 DATLVW010000000
VERSION     DATLVW010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34268"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VNN25_24670"
     CDS             complement(<1..301)
                     /locus_tag="VNN25_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94791.1"
                     /translation="MPDDLATIKRKQYNAHLAVMALRRTIFERGASARIDELIKAEER
                     LAQLSEERARAEKQSLAANASQAGGKLLGGATTGLDAKAELRLEYVPTALVHLFDS"
     gene            complement(301..1368)
                     /locus_tag="VNN25_24675"
     CDS             complement(301..1368)
                     /locus_tag="VNN25_24675"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH94792.1"
                     /translation="MPVEIIVLPPGTPDARPQMLLNGGGIWRDVTPSAGDAFSAKEFR
                     KKVETVLTIAMQQVSLNQQVNAMAQGFSSSFAPLYQELMPIEVRVAIASMFANEASPL
                     LRIHLHPSAEWIPWELLHDGQGFLGLRMQVVRMPVVTQAARIAGPTQRDVRSVYNLLG
                     NGALDAAIIPGWENTFATFNHGGWEKRCPNGGGQFASLDEFTSAAGADIIHITCHGGL
                     RDDENQPYYWSLDPDAPLYFNYRITSDTLAMLQFDRSLVFGNACSTAKGGKGVLHGFG
                     ALFMMKGALNFIGTLAPITQKTSVDFATAFYGNLLDPGKKFTIADALLETKKSFIANT
                     ADPSYLFYSLYGPPDILYRVP"
     gene            complement(1368..2768)
                     /locus_tag="VNN25_24680"
     CDS             complement(1368..2768)
                     /locus_tag="VNN25_24680"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HXH94793.1"
                     /translation="MIPNVGIDDLTSRIYERRPQETDAPPSDTAPYVLFLGDGCSEAA
                     GVPKLPQIAARAMQMFGLAKTATGSPHEELVGSFLDTITSMKKEQVARMMQMIFASIP
                     VPSFYQQLAQLVRERFFPLIITTNYDTLLEQALDSVGFRAFDYRVTTFDGVTPISTST
                     GGGSELVHIVKLHGDLARQVIHFTPEAVDSALRSSRQFVKSELRGDLIMVGFSFGDEA
                     INSWLSHSPSREVWWVAPKPPDNPQVVSSWAQEWHEISGTFGNPSIFFAQLSLRLLRL
                     TQFRVRGQERSISQTPDDESFSAALPQAPAELGLADTLQGEIRRAQNVLYALEQSAPV
                     GLRPSDVQAQIAYQKKQMSVLEEKMWSLEDVKPAVIEVVTEIRDDILKADLDQSNNFH
                     IGDSLRKYLFEQVDTVSHEMTKEKPDRFLVSASLSATLALADRVTTDYGSSIIRPNNL
                     RRLAEFAPLAAGRVIV"
BASE COUNT          553 a          895 c          976 g          586 t
ORIGIN      
        1 ccgaatcgaa gaggtgcacg agcgcggttg gcacgtattc caggcgcagc tccgctttcg
       61 catcgagccc ggtcgtggcc ccgccaagga gcttgccgcc ggcctgcgag gcgttggcgg
      121 cgagactctg cttctcagcc ctggcgcgct cctcactcag ttgcgccagc cgctcttcgg
      181 ctttgatcag ttcgtcgatg cgagcgctcg cgcctcgttc gaagatcgtg cggcgcaatg
      241 ccatcaccgc aaggtgcgcg ttgtactgct tccgcttgat ggttgccaga tcgtcgggca
      301 tcagggcacg cggtagagga tgtcgggcgg tccgtaaagc gaatagaaca gataggaggg
      361 atcggcggtg ttcgcgatga agctcttctt cgtttcaagc agtgcgtcgg cgatggtgaa
      421 cttttttccc ggatcgagaa ggttgccgta gaacgcggtg gcgaaatcga ccgacgtctt
      481 ctgcgtgatc ggagcgagcg taccgatgaa attcagcgcg cctttcatca tgaacagcgc
      541 gccgaagcca tgcagcacgc cctttccgcc cttagcggtc gagcaggcgt tcccgaatac
      601 gagcgagcgg tcgaactgca gcatcgccag cgtgtcggag gtgatgcggt agttgaagta
      661 gagaggcgcg tcaggatcga gcgaccagta gtacggctgg ttctcatcgt cgcggagacc
      721 gccgtgacag gtgatgtgga tgatgtccgc acccgcggcg ctggtgaact catcgagcga
      781 agcgaactgt ccgccgccgt tcgggcagcg tttctcccat ccgccgtggt tgaacgtcgc
      841 gaacgtgttt tcccagccgg ggatgatggc ggcatctaac gcgccgttgc ccaggaggtt
      901 gtacacggaa cggacgtcgc gctgcgtcgg accggcgata cgcgccgcct gcgttacgac
      961 cggcatgcgg acgacctgca ttcgcaggcc caggaatccc tgcccgtcgt gcagtaactc
     1021 ccacggaatc cactccgcac tcggatggag atgaattctg agcaggggcg acgcttcatt
     1081 cgcgaacatg ctggcgatcg caacgcgcac ttcgatcggc atcagctcct gatagagcgg
     1141 cgcgaacgaa ctggaaaagc cctgcgccat cgcgttgacc tgctggttga gcgagacctg
     1201 ctgcatggca atcgtcagta ccgtttcgac tttcttccga aactctttgg cgctgaacgc
     1261 atcgccggcg gacggtgtga cgtcgcgcca aatgccgccg ccattgagca gcatctgcgg
     1321 ccgtgcgtcg ggtgtgcccg gtggcagtac gatgatctcg accggcatca gacgatcacc
     1381 cttcccgcag ccagcggcgc gaactccgcg agccggcgca gattattggg gcggatgatg
     1441 ctgctgccgt agtcggtggt aacgcggtcg gcgagtgcga gtgttgcgct gagcgatgca
     1501 gagaccagga agcggtccgg cttctccttc gtcatctcgt ggctgaccgt atcgacctgc
     1561 tcgaacaggt acttccggag tgaatcgccg atgtggaaat tgttgctctg gtccagatcg
     1621 gctttgagga tgtcgtcgcg gatctccgtg actacttcga tgaccgccgg cttcacgtcc
     1681 tccagcgacc acatcttctc ctcgagcacg gacatctgct ttttctgata cgcgatctgg
     1741 gcctggacat cggaaggacg gagtccgacc ggtgcggact gctcgagtgc gtagagcacg
     1801 ttctgcgcgc ggcggatctc gccctgcaat gtgtcggcga gccccagttc cgccggcgcc
     1861 tgcggcagcg cggcggagaa cgactcatca tccggcgtct gcgagatcga tcgctcctgt
     1921 ccgcggaccc ggaactgcgt gagccgcagc aatcggagcg aaagctgcgc gaagaagatc
     1981 gagggattcc cgaatgtgcc gctgatctcg tgccactcct gggcccagga cgagacgacc
     2041 tgagggttgt ccggcggttt cggcgcgacc caccacactt cgcggcttgg actgtgcgag
     2101 agccagctgt tgatcgcttc gtccccgaag ctgaaaccga ccatgatcag atcgcctcgc
     2161 agctcggact tcacgaactg gcgactggac cggagcgcac tgtcgacggc ttccggcgtg
     2221 aagtggatga cctgccgcgc gagatcgccg tggagcttga cgatgtgtac gagctcgctg
     2281 ccgccgccgg tactggtcga aatcggtgta acgccgtcga aggtcgtgac gcggtagtcg
     2341 aaggcgcgga acccgacgga gtcgagcgcc tgctcgagca gcgtgtcgta gttcgtggtg
     2401 atgatcagcg gaaagaaacg ttcccgtacg agttgcgcga gctgctggta gaaggagggg
     2461 accgggatcg acgcaaagat catctgcatc attcgcgcga cctgttcctt cttcatcgat
     2521 gtgatggtgt cgaggaacga gcccacgagc tcctcatgcg gcgaaccagt tgcggttttc
     2581 gcgagcccga acatctgcat ggcgcgcgcg gcgatctgag gaagcttcgg cacgccggcc
     2641 gcttcgctgc agccgtcgcc gaggaagagg acgtacgggg cggtatctga cggaggagcg
     2701 tcggtctctt gcggccgacg ctcgtagatg cggcttgtca ggtcatcgat accgacattc
     2761 gggatcatca ggcgccctca tgtaaggtgt gtcggaaggg aggggtttcc ctacctcggc
     2821 gtagccgaaa ccaagaacgt cgggcggtga gctacacttc cgcgatcccg acaccaaaca
     2881 cgaagcgctt ccgggatctt gacttttcag ggctacggtc aactcacttg caggcaagaa
     2941 catcaaatta ctcgcggacg cgagaataag gccgccttcg tcataccgga tctccgcctg
     3001 acgaactggg
//
LOCUS       DATLVW010000843         8635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_34372, whole genome shotgun sequence.
ACCESSION   DATLVW010000843 DATLVW010000000
VERSION     DATLVW010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34372"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VNN25_24685"
     CDS             complement(<1..602)
                     /locus_tag="VNN25_24685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH94794.1"
                     /translation="MGSSVVIDRATVAGSNGNAIVRDVSLRIDPGESVAVIGRSGAGK
                     TTLLRLLNGLASPSTGTVVIDGLPLGEPGLAERRRRIGTILQAPALFPHRTVLENVAT
                     VPRLLGWPDERIRQASAALLERLDLPFDRFAGRFPRSLSGGEQQRVGIARAMVADPPL
                     LLCDEPFSALDPIVRRDLQDTFVGLRDGGSVTLLFVTHDVA"
     gene            complement(751..1401)
                     /locus_tag="VNN25_24690"
     CDS             complement(751..1401)
                     /locus_tag="VNN25_24690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94795.1"
                     /translation="MKKASPVFLPALALVMFFLVPTAFGQSASGGIHIVTDDGTRNIE
                     FDAKVDASGNASGQIKFTGPLSVPDQDVDGDGTGDPSATASTLSLRVEVDCLKVDGTR
                     AVLAGLVKESNVGAYIGRRMLLTVEDGGEGANAPPDRYTWGQYRSKTATWVASDAELD
                     FDPGVGLTWTATDFERTDDAGIPSNTPPGIDCKTFSLSSYALEEVPQGSGNVQVRP"
     gene            1774..4098
                     /gene="hypF"
                     /locus_tag="VNN25_24695"
     CDS             1774..4098
                     /gene="hypF"
                     /locus_tag="VNN25_24695"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXH94796.1"
                     /translation="MSSIANIAPTCRRRILVRGIVQGVGFRPFVHNLAHALDLRGFVR
                     NSASGVVIEVEGRGASVDRLLDSIRGHPPPLAQVVEISVSDLVPQQTPAFTIVDSTPD
                     DSALGLISPDVALCTDCRRELHDPSDRRFGYPFINCTHCGPRYTITQGLPYDRPATTM
                     RMFAMCARCLGEYEDPGDRRFHAQPNACPDCGPGLALVAGGDDPTSFDARNAMDVIER
                     VQTLLAGGAVVALRSVGGFHLACDAANDDAVVRLRAMKRRSEKPFAVMARDLASAERL
                     CVVSNADRALLLGIEQPIVIMERRADAAIAQSVAPNNRTLGVMLPYTPLHELLFRDAP
                     FDSLVMTSGNRSEEPIVTSNDDALRQFAGRADALLLHNRDIHMRVDDSVVRTFEGQPR
                     MLRRSRGYAPRPIDLGAPVAQILACGAELKNTLCLTRDHQAIVSQHIGDLSSYESMQF
                     FEDTLANLKTLFRIEPRIVAHDLHPLYASTRYACSLDGVERVGVQHHHAHIASCMADN
                     GIRGRVIGVAMDGTGYGTDGAIWGSEFLVADFSAFERRAHLRYVPLAGGDAAVRQPWR
                     SALSYLRDASVAGAARKLALFDEVPRSSVAIVEAMLARGLNTIPTSSCGRLFDAVASI
                     IGVRHAISFEAQAAIDLEMLAAGEPDDADAYPFVIALTDPMEIDVRPMIAEIVDEVLR
                     GTPTTRIASRFHATLAEVIADLCGRIRLRDGINRVCLSGGTFQNFVLLERTARLLRNS
                     GFELFLHSRVPTNDGGISLGQAVIANQLSIASGA"
     gene            4101..4367
                     /locus_tag="VNN25_24700"
     CDS             4101..4367
                     /locus_tag="VNN25_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HXH94797.1"
                     /translation="MCLAIPGKVVECFEKQDLKMARVQFGGIVREACLDYVPDAKVGD
                     YVLVHVGFALSRVDEEEALRTYALLEELDQLQELEAGYPEEAAS"
     gene            4364..5497
                     /gene="hypD"
                     /locus_tag="VNN25_24705"
     CDS             4364..5497
                     /gene="hypD"
                     /locus_tag="VNN25_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HXH94798.1"
                     /translation="MRFVDEYRDGRLARAIVRDIERMQTKPWVIMEVCGGQTHSIVKH
                     GIDHLLPPGIELVHGPGCPVCVTSLEMIDKAHAIAARPDVIFCSFGDMLRVPGSRADL
                     FTLKSEGADVRIVYSPLDCLAIAKANPDKKVVFFAIGFETTAPANAMLVWRAHQEGVK
                     NVSVLVSHVLVPPSIAAILQSPQNRVQGFLGPGHVCTVMGYREYEALSARFRVPIVVT
                     GFEPLDILEGVRMTVRQLEDGRSEVENQYARVLDREGNAAARDVIFRVFEICDRKWRG
                     VGSIPKSGYKLRHELRDHDAERLFQVSEIDTQEPAICISGQILKGIRKPHDCPAFGTL
                     CTPRTPLGATMVSSEGACAAYYAYGRHLQKQPPPVSRPVETQV"
     gene            5501..6109
                     /locus_tag="VNN25_24710"
     CDS             5501..6109
                     /locus_tag="VNN25_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXH94799.1"
                     /translation="MTDTIRETSWRASVMRKCGESVDVKEKFLGKYADTIGELCQIMA
                     ARFQAGHRLLVMGNGGSACDAQHVALEFNHPIIEKRRALPVIDLVSGTPLVTAISNDE
                     DFARVFVDQIKLWGQPGDMALAISTSGQSPNLIYAMAAAKEKGLLTIAFSGKDGGRLS
                     EVAEYCFTVPSYSIHRIQESHVALLHILWDLTHVALGEEDVI"
     gene            6109..7170
                     /gene="hypE"
                     /locus_tag="VNN25_24715"
     CDS             6109..7170
                     /gene="hypE"
                     /locus_tag="VNN25_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HXH94800.1"
                     /translation="MSEQVIDATMLGACPVPLTRHDRIVLGHGSGGKLSAELIETMFL
                     PAFRNEYLDRLDDQAVFDVNGSRLAFTTDSFVVTPIFFPGGDIGRLAVNGTVNDLAMS
                     GARPLYLSVAFILEEGLLMEELQRIVDSMRAAAAEARVLLVTGDTKVVNRGKGDKIFI
                     NTAGVGLIERPVTISASGAKAGDKIIVSGYIGDHGMAILSQRENLTFESDIESDTAPL
                     YDLVEGMLEIGGIHCLRDPTRGGVATTLNEIAQRSKVGIEIDEAAVPVRESVRGACEI
                     LGLDPLYVANEGKLVAIVDPVKADAVVAKMRTMKHGADARVIGTVVASPLTLVLLKTD
                     IGGTRVVDLLWNEQLPRIC"
     gene            7292..8353
                     /locus_tag="VNN25_24720"
     CDS             7292..8353
                     /locus_tag="VNN25_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HXH94801.1"
                     /translation="MSKVVAMQSNRPPAVSDIHILWMTAGLGCDGDTVSITAAEQPSI
                     EDVLFGAIPGLPKVHLHNPVVAFETGSDFMQHFYDAEKGKLDPFVLVVEGSIPNEKVH
                     KDPAGYWAAQGTDAATGQPIKTTDWIDRLAPKALAVVGAGTCATYGGIHAMAGNPTGC
                     MGLADYLGWNWKSKAGLPVINVPGCPVQPDNFMETLLYLLYQAAGLAPTIPLDDQLRP
                     TWLFGKTVHEGCDRGSYYEQGDFAPEYGSSRCLVKLGCWGPVVNCNVTKRGWMRGIGG
                     CPNVGGICIGCTMPGFPDKFMPFMDEPPGARISAFVIPVYGRIIRKLRAITNTTVNKE
                     PKWRYKGSRLTTGHSPQAY"
     gene            8370..>8635
                     /locus_tag="VNN25_24725"
     CDS             8370..>8635
                     /locus_tag="VNN25_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXH94802.1"
                     /translation="MTTLTEVKRESKTGKRNLVEMNWDPITRIVGSLGIFTKIDFANR
                     QVAECYSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHAT"
BASE COUNT         1621 a         2685 c         2673 g         1656 t
ORIGIN      
        1 gcaacatcgt gcgtgacgaa cagcaacgtg acgctgccgc catcgcgcag cccaacgaag
       61 gtgtcctgaa gatcgcggcg gacgatcgga tcgagcgcgc tgaacggctc gtcgcagagc
      121 agcagcggcg gatcggcgac catggcgcgg gcgatcccga cgcgctgctg ttcgcctccc
      181 gagagcgaac gcgggaagcg tccggcaaaa cgatcgaagg gcaagtccag gcgttcgagc
      241 aatgccgcac tcgcttgccg gatgcgctcg tcaggccagc ccaggaggcg aggaaccgtg
      301 gccacattct cgagcaccgt ccgatgagga aagagcgcgg gcgcctgcag gatcgtaccg
      361 atccgtcgcc gccgctcggc aagtcccggc tcacccagcg gcaagccatc gatgacgacc
      421 gttccggtac tcggcgaggc cagtccgttg agaagccgga gcagcgtggt cttgcccgcg
      481 ccactcctgc caatcaccgc caccgattcg cccggatcga tccgcagcga gacatcgcgc
      541 acgatggcat tgccattcga tccggccact gtcgcacgat cgatgactac ggaagagccc
      601 acgcggcgat tgtaaggtgg agagatcaga gaagcagaga tcagagatag cggagatcag
      661 agatcagaga tagcggagat cagagatcag gaatcttgga tgcaattctg gtctctgatc
      721 tctagtctct gatctctgct tcaaacagct ttatggcctg acctgaacat tcccgctgcc
      781 ctgcggcacc tcttcgaggg cgtacgacga gagggaaaag gtcttgcagt cgattcccgg
      841 cggagtgttt gaggggatgc ctgcatcgtc agtccgttcg aagtcggtgg cggtccatgt
      901 caggccgacg cccgggtcga agtccaactc cgcatcgctc gcaacccacg tggcggtctt
      961 cgatcggtac tggccccatg tgtagcggtc gggcggggca ttcgctcctt cgccgccgtc
     1021 ttcgacggtg agcagcatgc ggcggccgat ataggcaccc acgttcgatt ccttgaccag
     1081 gcccgccagt acggcgcggg tgccgtcgac tttcaggcag tcgacttcga cgcgcagaga
     1141 aagagtggac gccgtcgcgc tcggatcgcc ggtgccgtcg ccatcgacat cctgatcagg
     1201 aaccgagagc ggaccggtaa atttgatctg gcccgaagcg ttgccactcg cgtcgacctt
     1261 tgcgtcgaac tcgatattgc gagtgccatc gtcggtaacg atgtggatgc cgccactggc
     1321 gctctggccg aaggcggtcg gtacgaggaa gaacatcaca agggccaatg cagggagaaa
     1381 gaccgggcta gcctttttca tcgcaactcc ttacgaaaac agttgtgggg gtcccgacgg
     1441 aacgtaggtt cgggcttcgc cgggaattgt gattcgcatc acgttcgcgg aacggtgccg
     1501 gcaaaaagcc ggcagcaatt tccagaagtc gccggttaaa gcagccagcc tccatcatcg
     1561 acgagaggac gccgccggcg cccggcaagc agcgcgtgga tgaacgagat ccggcccgaa
     1621 ccaatcccag gccgatgtcg acgacgtcct cgtcgcgaga gagccggggt tgggctccgt
     1681 cattctccca ggcgccctcg cttgcgaatc acactcccgg cacgctgtga acagatcttg
     1741 cagtccccgt ttcctcgatt ttctcattgg cctatgtcga gcatcgcgaa catcgcccca
     1801 acctgccggc ggcgaattct cgttcgcggc atcgtgcagg gtgtgggctt tcgtcccttc
     1861 gttcacaacc tcgcgcacgc gctcgacctt cgcggtttcg tgcgcaactc cgcgtccggc
     1921 gtggtcatcg aagtggaggg acgcggcgcc tcggtcgacc ggctgctcga ttcgattcgc
     1981 ggacatccgc cgccgctggc gcaggtagtc gagatctccg tttcggacct tgttccgcag
     2041 cagactcccg cgttcaccat cgttgacagc acgcccgacg acagcgccct cggcctgatc
     2101 tcgccggacg tcgccctctg cacagactgc cgccgcgagc ttcacgatcc ctcggaccgc
     2161 cgcttcgggt atccattcat caactgcacg cattgcggac cgcgctacac gatcacgcag
     2221 ggccttccat acgatcgtcc ggccacgacg atgcgcatgt tcgcgatgtg cgcgcggtgt
     2281 ctcggcgagt acgaggatcc gggagaccgc cgctttcacg cgcaaccgaa cgcatgtcct
     2341 gactgcggtc ctgggctggc gctggttgcg ggcggcgacg acccaacctc gttcgacgcg
     2401 cgcaatgcaa tggatgtcat cgaacgcgtt caaacgctcc tggccggcgg tgcagtcgtg
     2461 gcgttgcgca gcgttggtgg tttccacctt gcctgcgatg cggccaacga cgacgctgtc
     2521 gtgcggcttc gtgcgatgaa gcgccgcagc gagaagccgt tcgcggtgat ggcgcgcgac
     2581 ctggcatccg ccgaacgtct ttgtgttgta tcgaacgccg accgcgccct gctcctcggc
     2641 atcgagcagc cgatcgtgat catggagcgc cgcgccgatg cggcgatcgc tcagagtgtg
     2701 gctcccaata atcgcaccct cggcgtgatg ctcccttaca cgccattgca cgagctgctc
     2761 ttccgcgatg cgccattcga ctctctcgtg atgaccagcg gcaatcgcag cgaagagccg
     2821 atcgtcacca gcaacgacga cgcgctgcgc cagttcgcag gccgcgccga cgcacttctg
     2881 ctgcacaacc gcgacattca catgcgcgtc gatgactccg tcgtgcgaac gttcgagggt
     2941 cagccgcgca tgctgcggcg ttcccgcggc tacgcccccc gccccatcga cctcggcgcc
     3001 cctgtcgcgc agatcctggc gtgcggcgcg gagctcaaga acacgctctg cctgacgcgc
     3061 gatcatcagg cgatcgtcag tcagcacatc ggcgatctct ccagctacga gtcgatgcag
     3121 ttcttcgagg atacgctcgc gaatctgaag acgcttttcc gcatcgagcc gcgcatcgtg
     3181 gcgcacgatc tccatccgct gtatgcatcg actcgctacg cctgttcgct cgatggtgtc
     3241 gagcgcgtcg gcgtgcagca ccatcacgcg cacatcgcca gctgcatggc cgacaacgga
     3301 atccgcggcc gcgtgatcgg cgtggccatg gatggaaccg gctacggtac cgacggcgcg
     3361 atctggggca gcgagtttct ggtcgcggac ttcagcgcgt tcgagcggcg ggcgcacctt
     3421 cgctatgtgc cgcttgcagg cggcgacgcc gcggttcgtc agccgtggcg ttctgcgctc
     3481 agttaccttc gcgatgcgtc cgttgcaggt gctgcgcgaa aactggctct gttcgatgag
     3541 gtgccgagga gcagcgttgc gatcgtggag gcgatgttgg cacggggcct caacaccatt
     3601 ccgacctcat cgtgcgggcg cctctttgat gcagtggcgt cgatcatcgg cgtccgtcac
     3661 gcgatctcat tcgaagcgca ggctgcgatc gacctggaga tgctggcggc aggcgagcct
     3721 gacgatgccg atgcctatcc gttcgtcatc gccctaacgg atccaatgga aattgatgtc
     3781 cgtccgatga tcgcggagat cgtggacgag gtactgcggg gaaccccgac gacgcgcatt
     3841 gcctcgcgat tccacgcgac gcttgcagaa gtcatcgccg atctctgcgg ccggattcgt
     3901 ctccgcgacg gcatcaaccg agtctgcctg agcggcggaa cgttccagaa cttcgttctg
     3961 ctcgagcgaa ccgcgcgctt gttgcgcaat tcaggattcg agctgtttct gcactcccgc
     4021 gtcccaacga acgacggcgg catctcgctc gggcaagcag tgatcgcgaa tcaattatca
     4081 attgcgtcag gagcgtgagc atgtgtctgg ccatccccgg caaggtcgtc gagtgctttg
     4141 agaagcagga tctgaagatg gcgcgcgtgc agttcggcgg catcgttcgc gaggcgtgcc
     4201 tcgattacgt gcccgatgcc aaagtcggcg attacgttct ggtgcacgtc ggcttcgctc
     4261 tcagccgcgt ggatgaagag gaggccttgc gcacgtacgc gcttctggag gagctcgatc
     4321 agcttcagga gttggaagct ggttaccccg aggaggcggc gtcatgaggt tcgtggacga
     4381 gtatcgggat gggcggcttg cccgcgccat cgtccgcgac atcgagcgca tgcagacgaa
     4441 accgtgggtg atcatggaag tctgcggcgg gcagacgcac tcgatcgtca agcatggaat
     4501 cgatcatctg ctgccgcccg ggattgagct cgttcacgga cccggatgtc cggtctgtgt
     4561 cacgtcgctg gagatgatcg acaaggcaca cgccattgcc gcacggccgg atgtgatctt
     4621 ctgctcgttc ggcgacatgc tgcgcgtacc cggttcgcgc gccgatctct tcacgctcaa
     4681 gtcggaaggg gcggacgtgc gcatcgttta ctcgccgctt gattgtctcg ccatcgcgaa
     4741 ggccaatcct gacaagaagg tcgtcttctt cgccatcggc ttcgaaacca cggcgcccgc
     4801 gaatgccatg ctggtttggc gcgcgcacca ggaaggcgtc aagaacgttt ccgtccttgt
     4861 ctcgcacgtt ctcgtaccgc cgtccattgc cgcgattctt cagtcgccgc agaatcgcgt
     4921 gcagggattc ctgggaccgg gacacgtttg cacggtgatg ggatatcgcg agtacgaagc
     4981 gctcagcgcc cgcttccgcg tgccgatcgt cgtcaccggt ttcgagccgc tcgacatcct
     5041 cgaaggcgta cgcatgacgg tccgccagct cgaagacggc cgaagcgagg tcgagaatca
     5101 gtacgcgcgt gtgctcgatc gcgaaggcaa cgctgccgcg cgcgacgtca tcttccgcgt
     5161 gttcgagatc tgcgaccgca aatggcgcgg cgtcggctcg attccgaaga gcggctacaa
     5221 gcttcgtcac gaactgcgcg atcacgatgc ggagcgcctg ttccaggtca gcgagatcga
     5281 cacgcaggag ccggccatct gtatcagcgg ccagattctg aagggaatcc gtaagccgca
     5341 cgattgccct gcgttcggaa cgctctgcac accgcgcacg ccgctcggcg cgacgatggt
     5401 ctcgtcggaa ggtgcgtgcg ccgcttacta cgcgtacgga cgccatctgc agaaacagcc
     5461 gccgccggtt tcccgcccgg tcgagacgca ggtctgaagc atgaccgata cgatcagaga
     5521 gacttcctgg cgcgcgagcg tgatgcgcaa gtgcggcgag tcggtcgacg tcaaggagaa
     5581 gttcctcggc aaatacgccg acacgatcgg tgagctttgc cagatcatgg ccgcgcgctt
     5641 ccaggccggg catcgcctgc tcgtcatggg caatggcggc agcgcctgcg acgcgcagca
     5701 tgtcgcgctg gagttcaacc atcccatcat tgagaaacgg cgcgcgcttc cggtcatcga
     5761 tctcgtcagc ggtacgccgt tggtgacggc gatctcgaac gatgaggatt tcgcgcgtgt
     5821 cttcgtcgat cagatcaagc tctggggtca gcctggcgac atggcgctgg ccatttccac
     5881 cagcggccag tcgccgaatc tcatttacgc aatggcggcg gcgaaagaga aagggctgct
     5941 gacgattgcg ttttcgggca aggacggggg caggttgtcc gaggtggcgg agtactgctt
     6001 tacggtaccg agctacagca ttcatcgcat tcaggagtcc cacgtcgcgc tgctgcacat
     6061 tctctgggat ctcacccatg tcgcgctggg agaggaggac gtcatctgat gagcgagcaa
     6121 gtcatcgacg caacgatgtt gggagcctgt ccggtccctc tcacgcgcca cgatcggatc
     6181 gtcctcgggc acggcagcgg cggaaagctc agcgccgagc tgatcgagac gatgttcctg
     6241 cctgcatttc gcaatgagta tctcgaccgg ctcgacgatc aggcagtgtt cgacgtcaac
     6301 ggttcgcgcc tggcctttac gacggattcc tttgtcgtca caccgatttt ctttccgggc
     6361 ggggacatcg gccggcttgc cgtcaatggc actgtgaacg atctggccat gagcggcgcg
     6421 cggccgcttt atctctcagt tgccttcatc ctcgaggaag ggctgctgat ggaggagctg
     6481 cagcggatcg tcgattcgat gcgtgccgct gctgcggagg cgagagtact gctcgtgaca
     6541 ggtgatacga aagtcgttaa tcgagggaag ggcgacaaga tcttcattaa cacggctggg
     6601 gtcggtctca tcgaacggcc ggtcactatt tccgcgagcg gcgcgaaagc cggcgacaag
     6661 atcattgtca gcggctatat cggcgatcac ggaatggcca ttctgtcgca acgcgagaat
     6721 ctgacgttcg agagcgatat tgaaagcgat acggcgccgc tttacgatct cgtcgagggc
     6781 atgctcgaga ttggcggcat tcattgtctg cgcgatccga cgcgcggcgg cgttgccacc
     6841 acgctcaatg agatcgcaca gcgcagcaag gtcgggatcg agatcgatga ggccgccgtt
     6901 cccgtgcgcg aaagtgtgcg cggagcgtgc gagattctcg ggctcgatcc gttatacgtc
     6961 gccaatgaag ggaagctcgt tgccattgtt gaccccgtca aggccgatgc ggtcgtcgcc
     7021 aaaatgcgga cgatgaaaca tggcgcggat gcgcgagtca ttggaacagt agttgcttcg
     7081 ccactgacct tggtgcttct taagacagac atagggggaa cgcgcgtagt cgacttactg
     7141 tggaacgaac aactaccgcg tatctgctga gagaaagacc gcctggaacc gacatttgat
     7201 ctcgcttcgt ttttcgaagc cgccgttctt tgagtcattc aaccgtcgtt gatcctctct
     7261 ccgttaaccc tctgaccctg gaggaaaacc catgtcgaaa gtcgttgcca tgcagagcaa
     7321 ccggccgccg gcggtttccg acatccacat tctctggatg accgcaggac tcggctgtga
     7381 cggcgatacc gtatccatca ccgccgccga gcagccaagc atcgaagacg ttctcttcgg
     7441 cgccattccc ggcctcccga aagtgcatct tcacaacccg gttgtcgcct tcgagaccgg
     7501 ctccgacttc atgcagcatt tctatgacgc ggagaagggg aagctcgatc cattcgtgct
     7561 cgtcgtcgaa ggatcgatcc ccaacgagaa ggtccacaag gatccggccg gctactgggc
     7621 ggcgcaaggt accgatgccg cgacgggcca gccgatcaag accaccgact ggatcgatcg
     7681 ccttgccccg aaagcactcg cggtcgtcgg cgcgggtacg tgcgccacgt acggcggcat
     7741 tcacgctatg gctggcaacc ccaccggctg catgggcctt gccgattacc tcggctggaa
     7801 ctggaagtcg aaagccgggc tgcccgtcat caatgtgccc ggttgtcctg tccagccgga
     7861 caatttcatg gagacgctcc tctaccttct ctatcaagcg gcgggattgg cgccgacgat
     7921 tccactcgac gatcaactcc gtccgacctg gctgttcggc aaaacagttc acgaaggatg
     7981 tgaccgcggg tcgtattacg agcagggcga ctttgcgccg gagtacggat cgtcgcgctg
     8041 cctcgtgaag ctcggatgct ggggcccggt cgtcaattgc aacgtcacca agcggggatg
     8101 gatgcgcggc atcggcggct gcccgaacgt cggcggcatc tgtatcggct gcacgatgcc
     8161 cggcttcccc gataagttca tgcccttcat ggacgagccg cctggcgcgc gcatctccgc
     8221 gtttgtcatt cccgtctacg gaagaatcat ccgaaagctt cgcgcgatca cgaataccac
     8281 ggtcaacaaa gagccgaaat ggcgttataa gggcagcagg ctcaccaccg gccactcccc
     8341 acaggcgtac tgagaaaaag gagacgacca tgacgactct cacagaggtc aagcgcgaat
     8401 cgaaaaccgg caagcgcaat ctggtggaga tgaattggga tccgatcaca cggatcgtcg
     8461 gcagccttgg catcttcacc aagatcgatt ttgcgaaccg gcaggtggcc gagtgttaca
     8521 gcacctcctc catcttccgc ggctacagca ttttcatgaa ggggaaggac ccgcgcgacg
     8581 cccatttcat tacgagccgc atttgcggta tctgcggcga caaccacgcc acctg
//
LOCUS       DATLVW010000844         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_369561, whole genome shotgun sequence.
ACCESSION   DATLVW010000844 DATLVW010000000
VERSION     DATLVW010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369561"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VNN25_24730"
     CDS             complement(<1..970)
                     /locus_tag="VNN25_24730"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXH94803.1"
                     /translation="MRAFNPFLKPLACIIAILSSIAVSAQTPPAPPPPAPASADTPSI
                     KIGATLFADYTYIASPASKDSDGNTIHPDSFNVSRAYLNVTGQLNHYLSFRITPDIAR
                     ETGSGSSLNGSQTFRLKYGYAQLNLDDWTTKGSWVRLGVQQTPLLDYTESIYRYRFQG
                     TMLIEREGFVTSSDAGLSGHYNFAGDYGDIHAGIYNGEGYSKAETNDQKALQIRATVR
                     PLPRSTLGKGLRVTGFYDDDNYVKGDARRRFLQQVTYEHTRFTAGFEHLTTKDRTSAA
                     GREISGNGWSVWLNPRLTKGWELLLRHDEMKPDSSTAQQRRRNIAGV"
     gene            complement(986..1852)
                     /locus_tag="VNN25_24735"
     CDS             complement(986..1852)
                     /locus_tag="VNN25_24735"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXH94804.1"
                     /translation="MIEPNAIRPLSDREFRSLQELIYREAGIHLSEAKKALVSGRLTR
                     RLRALGLDNFKDYYEQVEANADERTAMLDCISTNETRFFREPKQFEFLEATVLPHWRA
                     LGDAGTIPKKIRVWSAACSSGEEPYTLAMVLGTHFPDSDGWSVEILASDLSTRVLSAA
                     REGVWPIERSNDIPERFRKAWMLRGIRSEEGRMRAHPRLKSMIEFRRINLNDRNYAIR
                     ESFDLIFCRNVLIYFNRESKTDVINRLTQHLSPTGLLFLGHAESLTGTTHSLEHAGPT
                     AYRRSQRQQPAQ"
     gene            complement(1859..2407)
                     /locus_tag="VNN25_24740"
     CDS             complement(1859..2407)
                     /locus_tag="VNN25_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXH94805.1"
                     /translation="MNSTLVVKSLNQYLTFFLAGEEYALSILQVTDIIECGVVTRVPG
                     TPPWIRGVHNLRGTVVPVIDLAIKFGLPPTEITRRTCVVIVELHVDDEALVMGVMTDS
                     VHQVVELGPDQIQAAPAFGPRVRVDCVLGMATSNGKFVVILDIDKILASEEILTAVTA
                     AIEDQPSNGSAGLSTSAHVSVE"
BASE COUNT          430 a          694 c          807 g          497 t
ORIGIN      
        1 ccacaccggc gatgttccgg cggcgttgct gggccgtgga cgaatcgggc ttcatctcgt
       61 catgccgcaa cagcagttcc caacctttgg tcagacgggg attgagccac accgaccagc
      121 cgtttccact gatctcgcgg ccagcagccg aggtgcggtc cttcgtcgtg aggtgttcga
      181 agccggccgt gaagcgcgtg tgctcatacg tcacctgctg caaaaagcgc cgccgtgcgt
      241 ctcctttgac atagttgtcg tcgtcataga agccggtgac gcgcagccct tttccaagcg
      301 ttgagcgcgg caacgggcgt acggtggcgc gaatctgcag ggccttctgg tcgttcgtct
      361 ccgccttgct gtaaccctcg ccgttgtaga tcccggcgtg aatgtctccg tagtcgccgg
      421 cgaagttgta atgaccggag agtccggcgt ccgacgacgt gacgaatccc tcgcgctcga
      481 tgagcatcgt gccctgaaag cggtagcggt agatcgattc cgtgtagtcg aggagcggcg
      541 tctgctgcac gccgaggcgc acccacgagc ccttcgtcgt ccagtcgtcg agattcaact
      601 gcgcgtagcc gtatttcagc cgaaacgtct gcgagccgtt gagcgagctg ccgctgccgg
      661 tttcgcgcgc aatgtcgggc gtgatgcgga aggagaggta gtgattgagc tggccggtca
      721 cgttcagata ggcacggctg acgttgaatg agtccggatg aatggtgttg ccgtcgctgt
      781 ctttcgaagc aggagaggcg atgtatgtgt agtcggcgaa aagcgtcgca ccgattttga
      841 tcgatggcgt gtcggccgat gccggtgccg gtggcggtgg cgccggtggc gtctgtgcgc
      901 tgacggcgat gctgctgaga atcgcgatga tgcaggccag tggcttgaga aacggattga
      961 acgctcgcat ggaagctcct tttctttact gcgccggctg ctggcgctgc gatcggcgat
     1021 aggcggttgg gccggcgtgc tccaaagagt gagtggtgcc ggtcagactc tctgcatgac
     1081 ccaggaacag aagtccggtt ggtgacagat gctgcgtcag acgattgatg acgtcggttt
     1141 tcgactcgcg gttgaagtag atgagaacgt tgcggcagaa gatcagatcg aaggattcgc
     1201 ggattgcgta gttgcggtcg ttgagattga tgcgccgaaa ctcgatcatc gattttagcc
     1261 gcggatgggc gcgcatgcgg ccttcttcgc tgcggatccc gcgcagcatc cacgctttcc
     1321 tgaaacgttc ggggatgtcg ttgctgcgct cgatcggcca gacgccttcg cgggcggccg
     1381 agagaacgcg agtggacagg tccgaggcca ggatctcgac ggaccatccg tcgctgtcgg
     1441 gaaagtgtgt gccgaggacc atggccagag tgtacggctc ttcgccgctg gagcaggccg
     1501 cgctccagac gcggatcttc ttcgggatcg tgccggcgtc gccgagggca cgccagtgcg
     1561 gcaacaccgt cgcttcgaga aactcgaact gcttcggctc gcgaaaaaag cgcgtctcgt
     1621 tggtcgagat gcaatcgagc atggccgtgc gctcgtcagc attcgcctcg acctgttcgt
     1681 agtagtcctt gaaattgtcg agaccgagag cgcgcagccg ccgcgtcagc cgaccgctga
     1741 cgagcgcttt cttcgcctcg gacagatgaa tcccggcctc gcgatagatg agctcctgca
     1801 gcgaccggaa ttcgcggtcg gacaacgggc ggatggcgtt tggctcgatc atgatgttct
     1861 actcgacaga aacgtgggcg cttgtcgaaa ggccggcaga tccgtttgaa ggctgatcct
     1921 cgatcgccgc ggtaaccgcg gtcagaatct cctcggacgc gaggattttg tcgatgtcga
     1981 ggatcacgac gaacttgccg ttgctggtgg ccatgccgag gacgcagtcg acgcgaacgc
     2041 gcggaccgaa cgccggcgcc gcctggatct gatcgggccc gagctcgacg acctggtgta
     2101 cggagtcggt catcacaccc atgaccagcg cttcgtcgtc gacgtgcagc tcgacgatca
     2161 cgacgcacgt tctgcgcgtg atctcggtcg gcggcaggcc gaacttgatg gccagatcga
     2221 tcaccggcac gaccgttccg cgcaggttgt ggacgccgcg gatccagggc ggtgtgcccg
     2281 ggacccgcgt gacgacgccg cattcgatga tgtctgtgac ctgcaggatc gagagcgcgt
     2341 actcctctcc tgccaggaag aacgtcaggt actgattgag cgacttcacg actaaggtgc
     2401 tgttcatcgt cgtctcctag aagtgcgt
//
LOCUS       DATLVW010000845         5110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_531738, whole genome shotgun sequence.
ACCESSION   DATLVW010000845 DATLVW010000000
VERSION     DATLVW010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531738"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VNN25_24745"
     CDS             complement(<1..234)
                     /locus_tag="VNN25_24745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94806.1"
                     /translation="MAELFELNITFAGIITHLDGIVPGVPMRAVVPNALSVRFGEIWI
                     PDPLTPQLRRKEYYLMPHVAYISDASGTARPCGL"
     gene            complement(355..1176)
                     /locus_tag="VNN25_24750"
     CDS             complement(355..1176)
                     /locus_tag="VNN25_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94807.1"
                     /translation="MGTLTITFSGICTHFHDCLPWDQPGPKIPMRSVLPNASGSTTGG
                     FFGLVSVLKPPFQDIYVVMPHTATAANAATNPTLSSVLTGAKLEVLRVDERQPRFSLD
                     GDGYHLSNYVDAIEPSPSLLQDAACYVDFFYGTGVVKGTKGDPKSSLTTVVTIETHDD
                     VPQVQFSPFSGDPFVWETNQLFVANQDVHVTPPEYKYFDFLGNYLTTSSGIPGVLTQP
                     TPGMTDTPKPLTPLLLADRLRDLSDAIRRLGPNFTVSPPKWYVPLDESCSDSHVP"
     gene            complement(1164..4442)
                     /locus_tag="VNN25_24755"
     CDS             complement(1164..4442)
                     /locus_tag="VNN25_24755"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH94808.1"
                     /translation="MRSPSTHLDPETLAAFAEGQLDAAGRNEVIAHLDQCEECLNDVA
                     LVMPSAGAESEKRRFLRPTWIIAIAAVVVLAIALPALRHAIRPSSPTDTLVALAPRSA
                     RLVEPRLTGGFPWAGYHGPMRATGTTPDAQQLKLGGAAGDLIERAEHDQSAEAQHAAG
                     VAMVLVEKPDEAIAKLETAARASHDAKSWSDLAAARYATASKLGRASLYPQALAAADA
                     ALRIDPNLPEALFNRALILERLGLTDEAKHAWQRYLEADSSSEWATEARSRIAQLPAT
                     THASQFEHDRALLERAAEHGDTAAVRRYVDAYRESARRYADYVYLHNWAEALKRNDIT
                     EAARWLTMARHVGDALVAISGEALLRDAVRAVDAATPGQQRAIAEAQVLYYTGRTAYS
                     RGQRDAAVRDLLRAAEGFEAAHDPMALQARYYAAGARSAGNDAAGARADLERVLSIAN
                     AHPEYISLGGQVRWELGRALSNDDDWLRATVVLAEGAAMFHRCGERSSEAFTETILAY
                     AYRALDRDDDAWLVRLQSFTALSAEGEREYLATSIAVATYAELAVGHRDAALSLSVLE
                     SSIARGGARPQLAIKPLVNLALLQSLSGDTAAALQSAREAEGLARAVVDPELRAGWSA
                     QTDVATGGALAGSSPREAMEPLTRAIDYYTTHGASAGLPQPLLLRSRCSVRNGDAPAA
                     MRDLERGMTIVESHPAPIPGASAGAGILDAEHALFTDAIRLSLDRGDKASAFAFAERS
                     HGTPTTIPELQHRLAGTGTAVLEIVALPGELVTFAVAENDAVVVRRPATIETLASLAE
                     ESLSETGTVAAGKLYDELIQPVDAVLDRAREVVIVTDPRLATVPYAALYDSVHRTFLV
                     DRFAVSAASSAGSLEPDETRTAAPALAAIALPSGGADSRGLPDAENEVRDVVGLYARA
                     TSIPAAAARFAQFRAAAAGADIVHIAGHTERQPGGGEQALLFVGASGKLERVSWKTIV
                     ASPAPNRGVVVLAACETLRPPSSSATRALSLGAAFSAAGASDVIGTLTPIGDRDARPL
                     FGALHRRLAAGERPADALRAVQQEAITMEKTSGGRRAWRAIALLTRRIPAPPHRKELL
                     SWVR"
     gene            complement(4439..>5110)
                     /locus_tag="VNN25_24760"
     CDS             complement(4439..>5110)
                     /locus_tag="VNN25_24760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94809.1"
                     /translation="EGRSSFASYVAIVVRRLFLDQKRSEGRWYPSAEATRQGDAAITL
                     ERLIHHDRRPLSEALSIAREQHPEADIAQLEAAAASLPERAPRPRLVPVIDGDEDRFA
                     SSSSADDRVLAADLNRASDRTSHAVQEALAAMTAQDRVTLRLRFSKGMAISDIARALG
                     VAQRPLYRRVESMLKDLRASLERAGIDAAEAANLVASAGDRLDFGLQSGKTDLDQPSE
                     QSETQ"
BASE COUNT          865 a         1599 c         1758 g          888 t
ORIGIN      
        1 gagtccgcat ggccgggcag taccactcgc gtcgctgata tacgccacgt gaggcatgag
       61 gtagtactct tttctacgaa gctgcggagt gaggggatcg ggtatccaga tctcgccgaa
      121 acgaaccgat aaggcgttcg gcacaaccgc ccgcatcggg acgccgggta cgatgccgtc
      181 gagatgggtg atgatgccag cgaaggtgat gttcaattca aacaattcag ccacgacagt
      241 aactcccttc tgtgagggtg gaaatgcgcc tggtcaacag ggcgagccag ccgcgttcat
      301 gaactcggtc tcgctcgccg catagcgcca ccatctcgtt tgcgaaaaac tccctcatgg
      361 gacgtgactg tccgagcatg attcatcgag aggcacgtac catttggggg gggagactgt
      421 gaagttcggg ccgagccgcc gtatcgcgtc gctcagatct ctcaatctgt ctgcgagcaa
      481 tagcggtgtc agtggcttgg gagtgtcagt catgccggga gtgggctgcg tcaaaacccc
      541 cgggatgccg gaagaggtcg tgagatagtt tcccaggaag tcgaagtact tgtactccgg
      601 cggggtgacg tggacgtcct ggttggccac gaacagttgg ttggtttccc acacgaaagg
      661 atctcccgaa aacggtgaga actggacttg cggcacatca tcatgggtct ctattgtaac
      721 gacagtcgta agagacgact tcgggtcacc cttcgttccc tttaccacgc ccgtcccata
      781 gaaaaagtcg acgtaacacg ccgcgtcttg aaggaggctc ggcgacggct caatggcatc
      841 aacatagttg gagagatgat acccgtcgcc gtcgagagag aagcgtggtt gcctctcgtc
      901 cacacggagc acctccaact tggcgccagt gaggacgctg ctcagggtgg gatttgtcgc
      961 ggcgttggca gccgttgctg tgtgtggcat tacgacgtat atgtcttgaa aaggaggctt
     1021 cagcaccgag accagaccga agaagccgcc cgttgtgctg cccgatgcat ttggaagcac
     1081 cgaccgcatc ggaatctttg ggccgggttg atcccatggg aggcagtcgt ggaaatgcgt
     1141 gcagatacct gagaaggtga tggtcaacgt acccatgata gcaactcctt tctgtgtggc
     1201 ggcgcgggga tgcgccttgt caataaggcg atggcacgcc aagcccggcg gccgccgctg
     1261 gtcttctcca tggtgatggc ttcctgctgc acggcgcgga gcgcgtcggc agggcgctct
     1321 cctgccgcca gacggcggtg cagggcgcca aagagaggcc gtgcgtcgcg gtcgccgatc
     1381 ggggtcagcg tgccgattac gtcgctcgct cccgcggccg agaacgccgc gccgaggctg
     1441 agcgctcgcg tggcagagga ggaagggggg cggagcgtct cgcacgcggc aagcaccacg
     1501 acgccgcgat tcggcgccgg cgatgcgacg atcgtcttcc atgagacacg ctcgagcttt
     1561 ccggatgcgc cgacgaacaa cagggcctgt tcgccgccgc ccggctggcg ctcggtgtgg
     1621 ccagcgatgt gtacgatgtc cgcgccggcg gctgccgccc ggaactgcgc gaaccttgcc
     1681 gcggccgcgg ggatcgacgt cgcacgcgcg tagagcccaa caacgtcgcg cacttcgttc
     1741 tccgcgtccg gcagccctct cgaatccgcg ccgccgctcg gcagtgcgat ggcggcgagc
     1801 gcgggcgcgg cagttcgcgt ttcatctggc tcaagcgacc cggcgctcga ggcggccgat
     1861 acggcaaatc gatcaacgag gaatgtgcga tggacggagt catagagcgc ggcgtacggc
     1921 acggtggcca ggcgcgggtc ggtgacgatc actacctcgc gcgcgcggtc gaggacagca
     1981 tcgaccggct ggatcaattc atcatagagc ttgcctgcgg caacggtccc cgtttcggaa
     2041 aggctctcct ctgccagcga cgcgagcgtc tcgatggtgg ccgggcggcg gacgacgact
     2101 gcgtcattct cggcgacggc gaacgtcacc aactcgccgg gcagcgcgac gatctcgagc
     2161 acagccgtgc cggtgccggc caggcgatgc tgcaactcgg gaatcgtggt cggagttcca
     2221 tgcgacctct cggcgaacgc gaatgcgctc gctttgtcgc cgcgatcgag gctgaggcgg
     2281 atggcgtcgg tgaagagggc gtgttccgcg tcgagaatac cggcgccggc ggaggcaccc
     2341 ggtatgggtg caggatggct ctccacgatg gtcatcccgc gctcgagatc gcgcatcgcc
     2401 gccggagcgt ctccgttccg cacgctgcag cgcgaacgga ggagaagcgg ctgcggcaac
     2461 ccggccgatg caccgtgggt ggtgtagtaa tcaatggccc gtgtcagcgg ctccatcgcc
     2521 tcccgagggc tcgaccccgc cagtgcccca ccagtcgcga cgtctgtctg tgcgctccaa
     2581 ccggcccgca actccggatc aacaacggcc ctggccagac cttcggcctc gcgcgcggac
     2641 tgtagcgcgg cagccgtgtc gccgctcagt gattgcagca gcgcgaggtt cacgagcggt
     2701 ttgatcgcga gctgcggacg cgctccgcca cgcgcgatgg aactttccag caccgacagc
     2761 gaaagcgcgg catcgcgatg acccaccgca agttcggcgt aggtcgccac cgcaatactt
     2821 gtggccagat attcgcgctc gccctccgcg ctgagcgccg taaacgactg cagacgcacg
     2881 agccacgcgt catcatcgcg atcgagagcg cgataggcgt acgcgaggat ggtctcggtg
     2941 aatgcctcac tggagcgctc accgcagcgg tggaacatcg ccgccccttc ggcgagcacc
     3001 actgtggccc gcagccagtc gtcatcattg ctcagcgcgc gcccgagctc ccaccgcacc
     3061 tgacccccga ggctgatgta ctcgggatgc gcgttggcta tgctcagtac gcgctcgagg
     3121 tcagcgcgcg ctccggcggc gtcatttccc gccgatcgcg cgcccgccgc gtagtagcga
     3181 gcctgaagag ccatcgggtc gtgggccgct tcgaacccct ctgccgcgcg aagaagatca
     3241 cgcaccgcag cgtcacgctg ccctcggctg taggcggtgc ggcctgtgta atagaggact
     3301 tgcgcctccg cgatcgcgcg ttgctgcccg ggcgtggctg cgtcgacggc gcgcacggcg
     3361 tcgcgcagca gcgcctcgcc ggaaatagcg acgagcgcgt cgccaacgtg acgcgccatg
     3421 gtcagccagc gcgccgcttc ggttatgtcg ttgcgcttta gcgcctcggc ccaattgtgg
     3481 aggtagacat aatcagcgta gcgccgggcg ctctcccggt aggcatcgac gtatcggcgc
     3541 actgcggcgg tgtcgccgtg ctcggcggcg cgctcgagga gcgcgcggtc gtgctcgaac
     3601 tgcgatgcgt gcgtcgtcgc cggcaattgc gcgatgcgcg aacgcgcctc ggtagcccat
     3661 tctgatgaag agtcggcttc gaggtagcgc tgccaggcgt gcttcgcttc gtccgttaag
     3721 ccgaggcgct cgaggatgag cgcgcgattg aagagcgcct cgggaagatt tggatcgatt
     3781 cgcagcgcgg cgtcagccgc ggcgagcgcc tggggataga gcgacgcgcg cccgagcttc
     3841 gatgccgtgg cgtagcgcgc ggccgcaaga tcgctccacg acttcgcgtc gtgcgaggcg
     3901 cgggcggcgg tctccagttt ggcgattgct tcgtccggtt tttcgaccag caccatcgct
     3961 acgccggcgg cgtgttgcgc ctcggcgctc tgatcgtgct cggcgcgctc gatcagatct
     4021 ccggcggcgc cgcccagttt gagttgctgc gcgtccggag tggtgccggt tgcgcgcatc
     4081 gggccgtgat agccggccca ggggaatccg ccggtcaggc gcggctcgac aaggcgcgcc
     4141 gagcgtgggg ccagggctac gagggtgtcc gttggggagc ttggacgaat ggcatgccgc
     4201 aacgccggaa gcgcgatggc aagaaccact acggcggcaa tggcaatgat ccaggtcgga
     4261 cgcagaaagc gtcgcttctc tgattccgcg ccggccgagg gcatcacgag tgcgacgtcg
     4321 ttcaggcact cctcacattg atcgagatgt gcgatcactt cgttgcgtcc tgccgcgtcg
     4381 agctggcctt ccgcaaacgc ggcgagggtt tcgggatcga ggtgcgtgga cgggcttctc
     4441 attgcgtctc cgattgttcg gacggctggt ccaggtcggt tttccccgat tgcagaccga
     4501 aatcgagccg atcgccggcg gaggcgacga gattggccgc ttccgcggcg tcgattccgg
     4561 cgcgctcgag tgacgcgcgg agatccttga gcatcgactc gacgcggcga tagagcgggc
     4621 gttgcgccac tccgagggcg cgcgcgatgt cggagatggc catgcctttg gagaaccgca
     4681 gacgaagggt gacgcggtcc tgcgccgtca tcgcggcgag cgcttcctgg acggcgtgag
     4741 aagtccgatc cgaggcgcgg ttgagatcag cggccagaac gcggtcatcg gcggacgacg
     4801 agctcgcgaa tcgatcctca tcgccatcga tgacgggcac gagccgcggg cgcggtgcgc
     4861 gttcggggag cgacgccgcg gcggcttcga gctgcgcgat atcggcctcg ggatgttgct
     4921 ccctcgcgat gctcaacgct tcgctgagcg gccgccggtc gtggtgaatc aggcgctcga
     4981 gggtgattgc ggcgtcaccc tgccgcgtcg cttccgccga gggataccaa cgaccctccg
     5041 agcgcttctg gtcgaggaag agccggcgga ccacgatggc cacgtagctg gcaaaggatg
     5101 agcgtccctc
//
LOCUS       DATLVW010000846         5816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_79980, whole genome shotgun sequence.
ACCESSION   DATLVW010000846 DATLVW010000000
VERSION     DATLVW010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79980"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2818)
                     /locus_tag="VNN25_24765"
     CDS             complement(<1..2818)
                     /locus_tag="VNN25_24765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXH94810.1"
                     /translation="MRSRSLLLVAFLILILVPTLIFAQTNAGSLSGRVTDASGAGLPG
                     VTVTATNSATGFNRSVVTESDGSYRFQSLPVGTYEVTADLAGFGAVTTRNVEINVSTD
                     RALNVTLKQATVKEQITVTASSPLIETTPAIGTVVSQREIANLPLNGRQFANLGSLAP
                     GTTLSVNSDPTKPGQLTIALAGGSGRNVNFLVDGGDNTDDTIGGALQNYNIEAVQEFN
                     IQTQQYKAEYGRTTGGVLTVVTKSGTNDFEGSAYEFYRDKSLNEKTETEKLNGSAKSP
                     YKRDQYGASIGGPIVKDMIHFFATAERLERPTSYVIDTKGIYPSFDGQAVPTPFKDNL
                     LTAKVSADPSPTQFLQVRYGYQRNSDIYGASPIILPSALGTLTNDYHSLLLGHTWQIG
                     SNKVNDFIFQDTHFKNAILATSSDPALIYPSGVSVGQSINTPQTTTQIKRQYKDDFAW
                     SSTLGSMRNDFKVGANYIDEPLLGGDFSTGTSGQYTLKNDVQGSPVLDIAIFSGFAGY
                     NTPIKQYNYYAQDDISVNSKLTLNVGLRYDLWKGFDLNQTGNPIWKTLSTQTKYNEPF
                     LKAFQGGKGGQLKNDTNNWAPRLGFTYDVNGDSKNIVRGGAGRYYDFPYTNATILFPA
                     VAIQSTYGQSYSNHDANGIKNANGSFFQPGQPLPPNQQSGGTAVPNPNEIASPTLATP
                     YSDQVSLGYSWQVNPWLGLNIDGSHISYKDIPFRFRGNPLDPTTANIGGCPAAGCRRF
                     AAFGSFRIWDGQGFAKYNGVNIGGHARLGTQFELQGFYTYSHTTGNVLAGADEFRITD
                     AGHQGDLRSVRDQSIDPYDPLCDACTGPLNTDQKHRVTLSGLYKAPLGINASGIFRYH
                     SGTPYTDWNGADDVSCGPDKPNCKDGYAFDLAPGVTHVNSLRGASFSQFDIRISKEFK
                     VYRSYGVEVIGEVFNLLNSKNATGF"
     gene            complement(2880..4916)
                     /locus_tag="VNN25_24770"
     CDS             complement(2880..4916)
                     /locus_tag="VNN25_24770"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXH94811.1"
                     /translation="MTLRRAGCLLGLILLTTCREPQRHFNVLFVTLDTFRADRINTAT
                     PALSRLAREGVHFDAADSPVPLTLPAHASLLSGLLPLHHGLRNNGIGTFPANRDTLAT
                     LFSRSGYRTGAFVGSFILDHRFGLDRGFERYDDEIARDSSDGGGTFEAERPAGVVIDR
                     ALSWLRQTDARPFFAWVHLYDAHAPYAPPSPYPQTYDGEIAYVDAQVARLLAAVDRGN
                     TIIVIAGDHGESLGAHGELTHGLLLYEPTLHVPMIVAAPSLAAHVVRTPVSTTDIAPT
                     IASLAGTTFANAVDGHDLAADLRAGREPRAEAVYGETKYPATFGWSELASLRTGTAKL
                     ISAPSPELYDLDHDPGETVSVLSTQRRTYRDLTAHLEQLRATSVAAALATIDAETRAK
                     LASLGYVAPSGSQSVTPRDPKAAVQLFRAFEEATALINTGRSAAAVSSLEQLVRDDPG
                     NHVFRETLARALRQTGDKHRAIALYREAVALAPNDADAWYNLAAALQEAGDDRESEVA
                     LGEAAKRDPARPEVHNVRGTALAERGDLQAAEGEFRSVIAADPQNGRAYNNLGNVLRA
                     MNRNEEAAEAYRKSIALAPRYADPLNGLGALLVAGGQARESLPYFDAALRLAPDFFEA
                     ELNRAIALQVSGNKEEAAAALRRLLARLPSSAAYDRQRIACKTLLQRLSSDMRR"
     gene            5301..>5816
                     /locus_tag="VNN25_24775"
     CDS             5301..>5816
                     /locus_tag="VNN25_24775"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXH94812.1"
                     /translation="MLLVAVGAFAQTTGAIVGTVAQAGTPLPGVTVEARSPNLQGARV
                     ETTDAGGHFRLSLLPPGDYTVVASLSGFNTVTEKGVHVGLGSTVTLEVAISPAASERI
                     TVTGAAPVVDVTSSSTGTNVTSETMAALPVARNFTAVAQISPGTKTDASGTTVYGSTG
                     AENEYVIDGLNT"
BASE COUNT         1090 a         1694 c         1881 g         1151 t
ORIGIN      
        1 cgaacccggt tgcattcttc gaattcaaga ggttgaacac ttcaccgatc acctcgacac
       61 cgtaactgcg gtagaccttg aactcttttg agatccgaat atcgaactgc gagaacgagg
      121 cgccgcgcaa tgagttcacg tgagtaacac ccggcgcgag atcgaacgca tacccatcct
      181 tgcagttcgg cttatccgga ccgcaactaa cgtcatccgc tccgttccaa tcagtgtagg
      241 gggtgccgga gtgatagcgg aagatgccgg aggcgttgat tccgagcggc gctttgtaga
      301 ggccactgag cgtaacgcga tgcttctgat ccgtattgag aggccccgtg caagcatcgc
      361 agagaggatc gtacgggtcg atgctctgat cgcgcacgct gcgcaaatcg ccctggtgac
      421 cggcatcggt aattcggaac tcatcggcac cggccagcac gttgccggtc gtgtgcgagt
      481 acgtgtagaa gccctgcagc tcgaactgcg ttcccagccg cgcgtggcca ccgatgttca
      541 cgccgttgta ctttgcaaag ccctgcccgt cccagatgcg gaagctgccg aaggccgcga
      601 agcggcggca tccggcggct gggcagccgc ctatgttcgc tgtcgtcgga tcgagcgggt
      661 tgccgcggaa tcggaacggg atgtccttgt agctgatgtg cgacccgtcg atattcagac
      721 cgagccatgg attgacctgc cacgaatatc cgagagacac ctgatcggag tacggagtag
      781 ccagcgtcgg cgaggcaatc tcgttgggat tggggactgc ggttccgcca ctctgctgat
      841 tcggcggcag aggctgaccg ggctggaaga agctgccgtt ggcgttcttg atgccgttgg
      901 cgtcgtggtt cgagtagctc tgtccgtacg tcgactggat ggcgacagca ggaaagagga
      961 tcgtggcatt tgtgtacggg aagtcgtagt aacggccggc accgccgcga acgatgtttt
     1021 tcgagtcgcc attcacgtcg taggtgaagc ccaggcgcgg cgcccagttg ttcgtatcat
     1081 tcttcagttg cccgcctttg ccgccctgga aagccttcag gaacggctcg ttgtactttg
     1141 tctgcgtcga gagagtcttc cagatcgggt tccctgtctg gttgagatcg aaccccttcc
     1201 agaggtcgta gcggagaccg acgttgagcg tcagtttcga gttgacggag atatcgtcct
     1261 gtgcgtaata gttgtactgc ttgatggggg tgttgtagcc ggcgaaaccg ctgaaaatcg
     1321 cgatgtcgag gacaggcgaa ccctgaacgt catttttcag tgtgtactgg cccgaggttc
     1381 ctgtggagaa atcgccaccg aggagcggct catcgatgta gttggcgccg accttgaagt
     1441 cattgcgcat cgacccgaga gtcgatgacc atgcaaagtc gtccttgtac tgccgcttga
     1501 tctgtgtggt cgtctgcggc gtgttgatgc tctgcccgac gctgacaccg ctgggataga
     1561 tgagggcagg gtcgctcgac gtggccagga tggcgttctt gaagtgcgtg tcctggaaga
     1621 tgaagtcgtt gaccttgttc gacccgatct gccacgtgtg gccgagcaga agggagtgat
     1681 agtcattggt gagcgttccc agcgcgctcg gcagaatgat cgggctggcg ccgtagatgt
     1741 cggagttgcg ctggtagccg tagcgcacct gcaggaactg cgtagggctc ggatcggcgc
     1801 tcaccttcgc ggtcagcagg ttgtccttga atggcgtcgg aacagcctgc ccatcgaagc
     1861 tagggtagat cccttttgtg tcaatcacgt agctggtcgg gcgctcgagc cgttcggcgg
     1921 tcgcgaagaa gtggatcata tccttgacga tcggaccacc aatggacgca ccgtactgat
     1981 cgcgtttgta cggcgacttg gcgctgccgt tcagtttctc ggtctcggtt ttctcattga
     2041 ggctcttgtc gcggtaaaac tcgtaggcgc ttccctcgaa atcgttcgtt ccggatttcg
     2101 tgaccacggt caaaacgcct cccgtggtgc ggccatattc agctttgtat tgctgcgtct
     2161 ggatgttgaa ctcctgaacc gcttcgatgt tgtagttctg cagcgcaccg ccgatcgtgt
     2221 cgtcggtgtt gtcgccgcca tcgacgagga agttgacgtt gcggccgctg ccaccagcga
     2281 gggcgatggt gagctgtccc ggcttggtcg gatcgctatt gacggagagc gtcgttcccg
     2341 gcgcgagcga tccaaggttc gcgaactgac gtccgttgag cggaaggttc gcaatctcgc
     2401 gctggctgac gaccgtacca atggcaggag tcgtctcgat caacggcgag gaagcggtga
     2461 cggtgatctg ttccttcacc gtggcctgct tcagcgtaac gttcagcgcg cggtcggtcg
     2521 acacgttgat ctcaacgttt cgtgtcgtca cggcgccgaa gccggccagg tcggcggtga
     2581 cttcgtacgt tccgaccgga agcgactgga agcgatagga gccgtccgac tccgtgacca
     2641 cgcttcggtt gaaaccggtg gcgctgttcg tcgccgtaac ggtcacgcct gggagcccgg
     2701 cgccactcgc atcggtgacg cgtccggata acgatccggc gttcgtctgg gcaaagatta
     2761 aggtgggaac gagtatcagg atcaggaaag caacgaggag aagggaacga gagcgcacgg
     2821 tctacctcct aagtaaacgc aacatttgaa cgttgactga aatataccgc aaagatgagc
     2881 taccggcgca tgtctgatga cagtcgctgg agcaatgttt tgcaggcgat tcgttgccga
     2941 tcgtaggcgg cggacgatgg cagccgcgca agaagccggc gcagcgcggc cgccgcttct
     3001 tctttgttgc cgcttacctg caaggcgatg gcgcgattca gttccgcttc gaagaagtca
     3061 ggcgcaagac ggagagcagc atcgaagtac ggcagcgatt cgcgcgcctg tcctccagcg
     3121 accagcagcg cgccgagccc gttgagcgga tctgcgtagc ggggtgccag cgcgatcgat
     3181 tttcggtatg cctccgctgc ctcttcgttg cgattcatcg cgcgaagcac gttgccgagg
     3241 ttgttgtagg cacgcccgtt ctgcgggtct gctgcgatca cggagcgaaa ctccccttcc
     3301 gccgcctgaa gatcgccgcg ctccgccagc gccgtgccgc ggacgttatg aacctcgggg
     3361 cgggccggat cgcgtttggc agcttcgcca agcgcgacct cggactcgcg atcatcgccg
     3421 gcttcctgca gcgctgcggc aaggttgtac caggcgtccg catcattcgg tgccagagcc
     3481 accgcttcgc gatagagcgc aatggcacga tgcttatcgc cagtctgtcg aagcgcacgg
     3541 gccagcgtct cgcggaacac gtgattgccc ggatcgtcgc gcacgagctg ctcgagcgaa
     3601 gacaccgcag cggcggaacg ccccgtgttg atcaacgcag ttgcttcttc gaatgcacga
     3661 aagagctgca cggctgcttt tggatcgcgc ggcgtgacgc tctgtgagcc ggagggcgct
     3721 acgtacccga ggctggccag cttcgctcgc gtttccgcgt cgatcgttgc aagtgctgcg
     3781 gcgacgctgg tggcgcgcag ttgctcgagg tgcgcggtga gatcgcgata tgtgcgccgc
     3841 tgcgtgctca agacgctgac ggtctcgccg ggatcatgat cgagatcgta aagctccggc
     3901 gatggcgcgc tgatcaactt cgccgtacct gtgcgcagcg aagccagctc gctccatccg
     3961 aacgtggccg gatacttcgt ctccccgtat acggcctcgg cgcgcggctc ccgtcccgcg
     4021 cgcagatcgg ccgcgaggtc gtgtccgtcg acggcgttcg cgaatgtcgt ccccgccagc
     4081 gacgcgatcg tcggcgcgat gtcggtcgtg ctcaccgggg tccgcaccac atgtgcagcc
     4141 agcgatggcg cggcaacgat catcgggaca tgaagcgtcg gttcgtagag cagaaggccg
     4201 tgcgtgagct ctccgtgtgc gccgagggat tcgccgtgat cgccggcaat cacgatgatc
     4261 gtgtttcccc gatcgaccgc cgccagcaat cgcgccacct gggcgtcgac gtacgcgatc
     4321 tcgccgtcgt atgtctgcgg atacggcgac ggcggcgcgt acggagcgtg cgcatcgtag
     4381 agatggaccc aggcaaagaa cggacgcgca tcggtctggc gcaaccagct caaagcgcgg
     4441 tcgatcacaa cgccggcggg ccgctcggcc tcgaacgtgc cgccgccgtc gctcgaatcg
     4501 cgggcgatct catcgtcgta gcgctcgaat ccacgatcga ggccgaagcg gtgatcgagg
     4561 atgaaggagc cgacaaacgc gccggttcga tagcccgagc ggctgaacag ggtcgcaagc
     4621 gtgtcgcggt tcgccgggaa cgtgccgatg ccgttgtttc gaagaccgtg atgcaggggc
     4681 agaagacccg acagcaatga ggcatgcgct ggaagggtga gtggtacggg cgagtccgcg
     4741 gcatcaaagt gaacgccttc gcgagccagc cgggaaagcg cgggagtcgc ggtgttgatg
     4801 cgatcggcgc gaaacgtatc gagcgtgaca aaaagaacgt tgaagtgacg ctgcggttcc
     4861 cggcaagtcg tcagaagaat cagcccgagc aggcatccgg cgcggcggag ggtcacggag
     4921 ccattctgca cgatcgcagt tggttaggac accaacgatt cggatcgaaa atccgattta
     4981 ttgaaggcga ccacgctgcc agaccgagct ggtgcttgcg ctaaccacct aaaaatacta
     5041 cgctcgcgcc gccttgggct ccgtcagcac cggcggcagc gtgcatgcac gcttctcagg
     5101 tacagcagcc cggaaaagca gtccacgccg tcccatcagc acagccagtc aaatctacgg
     5161 aggtacgcca gcctcagagt gtgatcaagt cttacgtcag gtcaggataa cgcatcgcag
     5221 tccaaccgct gtatacgtca ggttaggaga atgaaaacat gttcttacaa aagtttcgag
     5281 ttgcactact gatagcgata gtgctcttgg tcgcggtcgg cgcatttgcg cagacaaccg
     5341 gtgcgatcgt aggcacagtc gctcaggcgg gaacgccgct tccgggcgtg accgtcgaag
     5401 cccgctcgcc gaaccttcag ggcgcgcgtg tggaaacgac cgacgccggg gggcacttcc
     5461 ggttgagcct cctgcctccg ggcgattaca ccgtcgtggc gagcctttcc gggttcaaca
     5521 ccgtaactga gaagggtgtt cacgtcggcc tgggcagcac cgtgaccctc gaagtggcga
     5581 tcagccccgc agcttcggag cggatcaccg ttaccggcgc ggctcctgtc gtcgacgtga
     5641 cctcgtcatc gaccggcacg aacgtgacca gcgagacgat ggcggcgctt ccggtcgccc
     5701 gtaatttcac ggcggtggcc cagatctcac cgggcacaaa gacggacgcg tcgggtacga
     5761 cggtctacgg ctccactggc gccgagaacg agtacgtcat cgacggtctg aacacg
//
LOCUS       DATLVW010000847         6168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_160988, whole genome shotgun sequence.
ACCESSION   DATLVW010000847 DATLVW010000000
VERSION     DATLVW010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160988"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VNN25_24780"
     CDS             <1..558
                     /locus_tag="VNN25_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94813.1"
                     /translation="RQDGTVPFERDLDRRTGISARLRMQIPERAMVQLTRVDNRGERD
                     EYRGEYAWQTHFNIAGAQLGLTSPATLAAEYCWGATGMGDAPHFVQADFATWYLLGSW
                     KRANDRFSLRFDTFSTKDRDHVAENNTEHGHSWVLAWMHDLKPNVRLGTEFAQVTGDR
                     PAAQQSGASPNTDGRSVTVELRYEF"
     gene            602..1531
                     /locus_tag="VNN25_24785"
     CDS             602..1531
                     /locus_tag="VNN25_24785"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXH94814.1"
                     /translation="MFLDLTILATMLLLNPARTNGAKIQRYPTLHTAGKTDRILIVAP
                     HIDDESIGAGGYAIDAIANGADVYVVFLTAGDCNRFAARLMFKTLEPTASNFLGIGNE
                     RIREAGMAMHLLGVPRDHYFVLGYPDRGLRTMVDNPSAIIRSKATHENSVPYADAVTP
                     RADYNYESMMRDLMKVVTIVKPTTVIAPVPFDHHSDHSAAAAITDAALEALHLHPSRL
                     GYLVHTARSRIHTAFMKTPRRAILPPARMKGFSWATYGLSTAVQQTKAEVLLTYKSQR
                     PYVFLLRNAFVRQNELFYLYPARQDPRIPIAEP"
     gene            complement(1545..2255)
                     /locus_tag="VNN25_24790"
     CDS             complement(1545..2255)
                     /locus_tag="VNN25_24790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94815.1"
                     /translation="MTLLRTLMVFSMLTALKYVSRIFFRYDISWVGDVPPDPWGHLRL
                     VAFLNHTSLYEPVFLGGVPNNFVWRLAAHGVVPAAEKTTGRPLVGIIFKFVAHHVVAI
                     TRERDHTWFQVLNRVDPKSMVVIAPEGRMKRANGLDVHGNPMSVRGGIADILLAVKEG
                     RMLIAYSGGLHHVQVPGKLPRAFKTVRLRLEIVDIATYIADMQRNGGADQFKKNVMQD
                     LDRRRDFYCPDEGLRKTA"
     gene            complement(2290..2994)
                     /locus_tag="VNN25_24795"
     CDS             complement(2290..2994)
                     /locus_tag="VNN25_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXH94816.1"
                     /translation="MHIRVGTSGYSYKEWKGTFYPDDLQAAKMLPYYGERFDSVEINN
                     TFYRMPDAKMVAKWGEQVPDGFTFVLKAPQRITHQKRLVGAGDDLRQLFEAGEVLGSK
                     LGPVLFQLPPFSRKDALKLREFVAILPPDRRVAFEFRHDSWFDEEIYAILRGRDIALC
                     AADTDEVTDPDALVVPTASWGYMRLRRTEYAPGQLTAWAQRVEKQAWSDAYVFFKHED
                     EGKGPRFAVEFRAQLH"
     gene            3190..4170
                     /locus_tag="VNN25_24800"
     CDS             3190..4170
                     /locus_tag="VNN25_24800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94817.1"
                     /translation="MPSFIVPTNANLHAAKSFIAQNNFFDAQGNAVLRFHPKWAHLEP
                     LALTMIAAWGAWCRREGYPLRVENLGKHAAYAARMKLFYQLGVEFDTGIREYEESGRF
                     LPVTQVRTQEDSTAAIANISAMLHLDDDPDSLAAVRFCISELLRNVLEHANSPDGAFV
                     AAHRFADKGTHRVTIAVADCGQGIPSHLARAHPETVGNDSIGIGLAMQPGVTGAQPST
                     YGTPNNAGAGLYITRSIAKGTGGYFLLYSGHAAYRLRRALSEDDMMQLYPDPYDDPRG
                     DRWSFPSPWLGTVVSVEIRTETIAHYENFFQWIFKQMPNRKSAAGKIRFT"
     gene            4167..4478
                     /locus_tag="VNN25_24805"
     CDS             4167..4478
                     /locus_tag="VNN25_24805"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HXH94818.1"
                     /translation="MSNEIRIESGVDGFAENKDDAIRLRVSQVLPTVSKGDNVVLDFS
                     AVKYATQSFIHALVGEALQTYGERALDLIEFRNCSDAVRSVVELVVGYSLSGFAEKAS
                     A"
     gene            4578..5036
                     /locus_tag="VNN25_24810"
     CDS             4578..5036
                     /locus_tag="VNN25_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXH94819.1"
                     /translation="MPTITIETLLPAPPERCFDASRDLDLHVTSMKETAEKAVAGRTS
                     GLIELGEQVTWEARHFGVRQRFTSKITAFDRPAYFQDSMVKGAFHSFVHDHYFEAVDG
                     GTKMIDVLTFASPLGFIGAIVDRLVMSGYLRRMLIARNDALMTALSQDIG"
     gene            complement(5067..5864)
                     /locus_tag="VNN25_24815"
     CDS             complement(5067..5864)
                     /locus_tag="VNN25_24815"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXH94820.1"
                     /translation="MPELPEVELYCRYFAGHALGRRVVAVRVLDARILGVREHVLRRA
                     LIGHEFVDVRRHGKHLFASAGTLWLHLHFGMSGDLAFYRDDTDAPRFARVVFDFEDGN
                     HLAYDDMRLFGVVDVTPDPDAFIAEHRLGPDPLDPAFRLRDFREIVSKRRGAVKSLLM
                     SQEIIAGMGNLYADETLYRSSIHPRRPVDGLSDAEVKTIFTNMRAILKEAIAFKSRGG
                     DYPGRYLVQHREEGDRCPQCGGTIQRTVVFGRTTYFCARHQPLPRRK"
     gene            5863..6153
                     /locus_tag="VNN25_24820"
     CDS             5863..6153
                     /locus_tag="VNN25_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94821.1"
                     /translation="MRWEVYDASNSELRSSMARVPDAEVSQLDRSIRAVLEAQAKRWG
                     APLLNHLIYARRPSIFKGARMMWSGLDVSGLIDGKLTALINRRVAFLNGCEF"
BASE COUNT         1232 a         2002 c         1790 g         1144 t
ORIGIN      
        1 cgacaggatg gaaccgtgcc cttcgagcgc gatctcgacc gccgcactgg gatctcggca
       61 cgcctgcgga tgcaaatccc cgagcgcgcc atggtccagc tcacgcgcgt cgacaaccgc
      121 ggcgagcgcg acgagtatcg cggcgagtac gcgtggcaga cgcacttcaa catcgccggc
      181 gcgcaactcg gtctcacatc gccggccact ctcgccgccg aatactgctg gggggcgacc
      241 ggcatgggag acgcgccgca tttcgtgcag gccgactttg ccacctggta tctcctcggc
      301 tcctggaaac gagccaacga ccgcttctcg ctccgcttcg acacgttctc gaccaaggac
      361 cgcgaccacg tcgccgaaaa caatacggag cacggccact cgtgggtcct ggcgtggatg
      421 cacgacctca agcccaacgt ccgcctcggc acagagttcg cgcaagtcac cggtgaccgt
      481 cccgctgccc agcaatccgg cgccagcccc aacaccgacg gccggtccgt gaccgtggag
      541 ctgcggtacg agttctgaag aattccgtat aatcctcctc ggttacccgc ccgcgactgc
      601 gatgtttctc gatctgacga tcctcgccac gatgctcctg ctcaatcctg cgcgcaccaa
      661 cggcgcgaaa atccagcggt accccaccct ccacacggca ggcaagacgg accgtatcct
      721 catcgtcgcc ccccacatcg acgacgaatc gatcggagcg ggcggttatg ccatcgacgc
      781 catcgccaac ggcgccgatg tttacgtcgt ctttctcacc gccggcgact gcaaccgctt
      841 cgcggcccgg ctgatgttca aaacgctcga gccgaccgcg tcgaacttcc tcggcatcgg
      901 caacgaacgc atccgagaag ccggcatggc catgcatctc ctcggcgtcc cacgcgatca
      961 ctatttcgtg ctcggatatc ccgaccgcgg ccttcgcacg atggtcgata accccagcgc
     1021 catcatccgc tccaaggcga cacacgagaa cagtgtgccc tacgccgacg cggtcacccc
     1081 tcgggccgat tacaactacg aaagcatgat gcgcgatctc atgaaggtcg tgaccatcgt
     1141 caagcccacc acggtgattg caccggttcc gttcgatcac cactccgatc acagcgccgc
     1201 cgccgcgatc accgacgccg cgctggaagc gttgcacctc catccgtcgc gcctcggcta
     1261 cctcgtccac accgcccgca gccggattca caccgccttc atgaagactc cgcgccgtgc
     1321 catcctcccg ccggcgcgaa tgaagggctt ctcatgggca acctacggac tgtcgacggc
     1381 cgtgcaacag acgaaagccg aggttctgct gacgtacaag agccagcgcc cctacgtctt
     1441 cctcctccgc aacgctttcg tgcgccagaa cgagctgttc tatctgtatc cggcacggca
     1501 ggaccccagg attccgatcg ccgagccgtg atcggcgccg gactctacgc cgtcttccgc
     1561 agcccttcgt ccgggcaata gaaatcgcgc cgccggtcga ggtcctgcat cacgttcttc
     1621 ttgaactgat cggcgccgcc gttgcgttgc atgtcggcga tgtacgtggc gatgtccacg
     1681 atttccagcc gcagccgcac cgtcttgaac gcgcgcggaa gtttgcccgg cacctgcacg
     1741 tgatgcagac cgccgctgta cgcgatcagc atccgtcctt ctttcaccgc cagcagaatg
     1801 tccgcgatgc cgccgcgcac actcatcgga ttgccatgca cgtccagacc gttcgcacgc
     1861 ttcatgcgcc cttcgggagc gatgaccacc atcgacttcg ggtcgacgcg gttcaacacc
     1921 tgaaaccacg tgtgatcgcg ctcgcgtgtg atggcgacga cgtgatgcgc gacgaacttg
     1981 aaaatgatcc cgacgagcgg ccgtccggtc gtcttctccg cggcagggac gacgccatgc
     2041 gcagcgagcc gccagacgaa attgttggga acgccgccga ggaagacggg ctcgtagagg
     2101 ctggtgtggt tgaggaaggc tacgagacga aggtggcccc acggatccgg cggcacgtct
     2161 ccaacccaac tgatgtcgta tcgaaagaag atgcggctca cgtacttgag cgcagtcagc
     2221 atcgaaaaca ccatcagggt acgcaggagt gtcacgagca caaagggtaa cgacgtaacg
     2281 ggcgcgccgt caatgaagct gcgcgcggaa ctccaccgcg aagcgcggcc ccttcccctc
     2341 atcctcatgt ttgaagaaca cgtaggcgtc gctccaggcc tgtttctcga cacgctgcgc
     2401 ccaggcggtg agctggcccg gcgcgtactc ggtgcgtcgc aaacgcatgt agccccacga
     2461 agcggtgggg acgacgagcg cgtccggatc ggtcacttcg tctgtgtctg cagcgcagag
     2521 cgcgatgtcg cgcccgcgga ggatggcgta gatctcctcg tcgaaccagg agtcgtgccg
     2581 gaactcgaac gccactcgcc gatcgggcgg caggatggcg acgaactcgc gcaacttgag
     2641 cgcatccttc cgcgaaaacg gcggaagctg aaagagaacc ggaccgagct tcgatccaag
     2701 cacttccccc gcttcgaaca attgccggag atcgtccccc gcaccgacga gtcgcttctg
     2761 atgcgtgatc cgttgcggcg ccttcaacac gaacgtgaac ccatccggca cctgctcccc
     2821 ccacttcgcc accatcttcg cgtccggcat ccggtagaac gtgttgttga tctcgaccga
     2881 atcaaacctc tcgccgtagt aaggcagcat cttcgccgcc tgcagatcgt ccggatagaa
     2941 cgtccccttc cactctttgt agctgtagcc gctggtaccg actcggatgt gcatggggtg
     3001 ctaatcctgc cacacgttcg ccaaacacga cgacgaagcg cgcgcgaccg actctcggct
     3061 atgagcgctt ccgtcgttcg ctatccgcgt tcaaggggtc cagtccccgc cgtcgcatcg
     3121 atccttgccg ctctggaaac ctctggccga ggcgaaagtg gtgctgagcc gatgtattct
     3181 ctccaaggga tgccttcctt catcgtacca acgaatgcga atctacacgc agcgaagtcc
     3241 ttcatcgcgc agaacaactt cttcgacgct caaggcaacg ccgttctccg ctttcatccc
     3301 aaatgggccc atctagagcc tttggcgctc accatgatcg cggcatgggg tgcctggtgc
     3361 cgccgtgagg gatatccgct aagagtggag aatctgggca agcatgctgc ctatgcggct
     3421 cggatgaagc ttttctacca gcttggtgtc gagttcgaca cgggcatcag ggagtacgaa
     3481 gaatcgggaa ggttccttcc tgtgactcaa gtgagaacgc aggaggattc cacagcggca
     3541 atcgcgaaca tctctgcgat gcttcacttg gacgacgatc ccgatagcct cgccgctgtt
     3601 cggttttgca tcagcgagct tctccgcaat gtgctcgaac atgcaaattc gccggacgga
     3661 gcctttgttg ctgcgcatcg attcgcagac aaaggaaccc acagggtcac aatagcggta
     3721 gccgactgcg gccaggggat accgagtcat ttggctcgag ctcatcccga gacagttggc
     3781 aatgactcaa tcggtatcgg gctcgcgatg caaccgggtg taacgggtgc gcaaccatcg
     3841 acctatggca ctccaaacaa cgctggcgcg ggtctttaca ttacgcggtc gatcgcaaag
     3901 ggcacaggcg gttacttcct gctgtattct ggtcatgctg cctatcgcct gaggcgggct
     3961 ctttcggaag acgacatgat gcaactgtat ccagatccct acgatgatcc acgcggagac
     4021 cgatggtcat tcccttctcc atggcttggt accgtcgtga gcgttgaaat tcgaacagag
     4081 acgattgcgc actacgagaa cttcttccaa tggattttca aacagatgcc gaatcggaag
     4141 tccgcggcag gaaagatcag attcacatga gcaatgaaat ccgaatcgag tccggtgtcg
     4201 atggtttcgc cgagaacaag gacgatgcaa ttcggctgcg ggtgtcacaa gtcctaccca
     4261 ccgtctcaaa aggggacaat gtcgtactgg acttttccgc ggtcaagtat gcgacgcagt
     4321 cgttcattca tgcgctcgtc ggggaggctt tgcagacgta cggcgagcgc gccctggacc
     4381 tgatcgaatt ccgcaactgc tccgacgcgg ttcggagcgt agtcgaatta gttgtcggct
     4441 actctctgag tggattcgca gagaaagcca gcgcctgaca tcttgtattc accgagcctt
     4501 gccgacgcag cgtggtgaat cttccaagtg gaacggcacc gccggcgtga cgccgggtga
     4561 ggctcatctc aaaacaaatg cccaccatca ccatcgaaac cctcctcccc gccccgcccg
     4621 agcgatgctt cgacgcgtcg cgcgatctcg accttcacgt tacctcgatg aaggaaacag
     4681 cggagaaggc ggtcgccgga agaacgtccg ggctcatcga gttgggcgag caggttacgt
     4741 gggaggcgcg gcatttcggc gtgcggcagc gattcacttc gaagatcacg gcgttcgaca
     4801 ggccggcgta ctttcaggat tcgatggtca agggggcgtt ccattccttc gttcacgatc
     4861 actatttcga agcggtcgac ggcggaacga agatgatcga cgtgctgacg ttcgcttcgc
     4921 ccctcgggtt catcggagcg atcgtcgacc gactggtgat gagcgggtat ctgcgacgca
     4981 tgctcatcgc gcgcaacgac gccctcatga cggcgctatc gcaagacatc ggttgaggcc
     5041 gacaaccagc gcaagcggcg ccggtctcac ttccgccgag ggagcggctg gtgcctcgcg
     5101 cagaagtagg tcgtgcgtcc gaacacgacc gtgcgctgga tcgtcccgcc gcactgcggg
     5161 cagcggtcgc cttcttcgcg gtgctgcacc aggtagcggc ctggataatc gccgccgcgc
     5221 gacttgaagg cgatcgcctc tttcaggatc gcgcgcatgt tcgtgaagat cgttttgacc
     5281 tcagcatcgg acaggccgtc gactggcctt cgcggatgga tcgacgagcg atacagcgtc
     5341 tcgtcggcgt agagattgcc catcccagcg atgatctcct gcgacatcag cagcgacttc
     5401 acggcgccgc gccgcttgct tacgatctcg cggaagtcgc gaaggcgaaa cgcaggatcg
     5461 agcggatcgg gcccgagccg gtgctcggcg atgaacgcgt ccggatcagg cgtgacatcg
     5521 acgacgccga aaaggcgcat gtcgtcgtac gccagatgat tcccatcctc gaaatcgaaa
     5581 actacgcgcg cgaatcgagg cgcatcggta tcgtcacggt agaacgccag atcgccgctc
     5641 atgccgaaat gcaggtgcag ccacaaagtc ccggcactgg cgaagaggtg cttgccgtgc
     5701 ctcctgacat ccacgaactc gtgaccgatg agcgcgcgtc gcagcacgtg ctctcgcacg
     5761 ccgagaatgc gggcgtcgag cacgcgcacc gcgaccacgc ggcggcccag cgcgtgtccg
     5821 gcaaagtagc ggcaatacag ttcaacctca ggaagctcag gcatgcggtg ggaagtgtac
     5881 gatgcctcaa actccgagct gaggtcatcg atggcgcgtg ttccggatgc ggaagtcagt
     5941 caactcgatc gttccattcg cgcggtcctg gaggcgcagg caaaacgctg gggcgcgccg
     6001 ctgctgaatc acctgatcta tgcgcgccgt ccctcgattt tcaaaggcgc ccgcatgatg
     6061 tggagcggcc tcgatgtctc gggtttgatc gacggcaagc tcacggcgct gatcaaccgc
     6121 cgcgttgcgt ttctcaacgg ctgcgaattc tgacaggaca tcaacgct
//
LOCUS       DATLVW010000848         6720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_80001, whole genome shotgun sequence.
ACCESSION   DATLVW010000848 DATLVW010000000
VERSION     DATLVW010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80001"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VNN25_24825"
     CDS             <1..1017
                     /locus_tag="VNN25_24825"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH94822.1"
                     /translation="RKNLPVATLGDSDSTRVSESIWAFGYPAVASSTDDVIGGWLSRD
                     SDLETTLSPGIITAIKRNVANTPVFQSNVAIYQGNSGGPAVNRNGEVVGISTWGRSDA
                     EAIKFLVPINVARSFLAEAKVPINLQGEFDRHYRASLDAAQDGKWTVASSELASANSL
                     FPNSPDLIHFRRDTERALQSMPLWRKHPVATAGAGGALAALALGLTLPRIRRPRIPAD
                     MLKVTTERVVSPSSAPPGAPQMLGKLTILNGPRAGERLGLGGSGIRIGRESAMCEIVL
                     ENPKVSRLHAEVVSIDGKVLLIDRNSSNGTYVNDHKIDKRFLKDGDIIYFGGRNAVAI
                     AFHA"
     gene            1028..1666
                     /locus_tag="VNN25_24830"
     CDS             1028..1666
                     /locus_tag="VNN25_24830"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH94823.1"
                     /translation="MADKRCENGHFIDESWDLCPYCPVDDAEPDVPVVRPTRFGAQDG
                     QRPAPIPVEPPRRPTAVPAPKTMARVDAGVAFEAEPADRTVAVQKLDPTAQPKRYVVA
                     WLIGLNGAARGESFPVRIGRNVLGRDRRSDVVVNDDQASSHHADLVFRPEERRFILMD
                     HNSTNGTYVNEAEIEPRRDLVAKDIVRVGSHKFLFVPLCEEGFYWDEEGVLK"
     gene            1663..2880
                     /locus_tag="VNN25_24835"
     CDS             1663..2880
                     /locus_tag="VNN25_24835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HXH94824.1"
                     /translation="MIGNVVGNYRIERELGEGGMSHVYVGRTVARSEILPEGYEVVLK
                     VINEELAGEVTARKRFVKEAHILEKLRHRYITRFYEFINKSDSAVLVMELVEGTPVDV
                     LLTERGALPIADAIGTAQCLLEALVYAHGKGIVHRDIKPANLIRENAGVVKVTDFGIA
                     KIKEGGTGSTQTVLTKSGFLLGTPHYMSPEQIREPKDAGAKSDVYSTGVVLYEMLTGS
                     LPFNSRSLPKLIDAIYRGEKQPPRALRPEIDAELEAIVLRAMHPRMEQRYESARAFFE
                     ALEEYNLRPGYTPTVRPMPAAQMPAGKAAAPQQWQLVHVKPEANEKHSLQGDSIMVGR
                     DRTCSVVLTHPAVSRRHARITMNGGACMLEDLKSANGTYVNNTRVEKIALKPGDVVRF
                     GADPACSYVLRSQ"
     gene            3035..4867
                     /locus_tag="VNN25_24840"
     CDS             3035..4867
                     /locus_tag="VNN25_24840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH94825.1"
                     /translation="MSQINCLRCKKPIDMELDQCPACGERVTMFQRTYSSKLLDGKYQ
                     ILDRLGVGGMGEIFKVRHIHLNELRVIKIMRPNVAADDQGMQRFLQEARTSTMIKHKN
                     LAMLYDFAQLDDGSYYMVWEFIDGTNIQKWIGQNGAMPSRLAIEISIQALSGLDHLHS
                     MGLIHRDISPENIMLSQDHHGKLLVKVIDFGIAKQLAEGEGGQGLTQTGMFLGKLKYA
                     SPEQAGFLKEGEHLDPRSDLYSFGIVMYEMLAGRAPFQATNPHGYILKHVTEKPLPIH
                     EINPAVKVPAQVEAVIMRSLEKNRDNRYATAADFEHALEAIRNTVPPDAKYGLGERMI
                     TLSGAQTLAELPKFTTGPSGTFSGQQATQQTMTGQPTQATQAGSMQAPRTGPTGTKIG
                     SDEATVMERNLGPANTMPGAPGTRASSDEATVMERLTSQAAPTAAEQKWAGGNAEATM
                     IERQVTPGSFAPKKSNTGMMIGIAAAVVIVLGVAFFVMRNKQSSAPPAAVPSTTSVTA
                     TAPPPSAPIAGGQGALLLSASPWGELEAIVDDKGKTVDLTDEKKDMPTRIDLEPGRYM
                     VTVAGPNGTKQTVPVDIEAGKRNRKVIKTGDVNFDELEKEVAKP"
     gene            4864..5925
                     /locus_tag="VNN25_24845"
     CDS             4864..5925
                     /locus_tag="VNN25_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH94826.1"
                     /translation="MKFARTFTIGLIGLLLATVALASWYDDYDAGIAAVGKGQWSTVI
                     QKMTAAINGHPKESDHERTYGAIFITYHPYYYRGVAYLNTGKYEQAISDLEKTSGPGE
                     ADLGPLGDLLRRAKSKLEAASTPEPQPQPQPQPVAPQPRVVPTPVPMPVQPVAPSIDP
                     ALRQQAAAAINQARTRLAAAQQRKAGNTPQYTQATQLLTDALTRSANPRSNDDLTNAI
                     GAAQNAAQIADLAPAPGAPIPPPVTIPTRPAQASAIVLADPTRRVRDALESYFRGEFD
                     DASVKFKTLSQEMPKNAWIWAFLGASQYSQYAFEADDTYKRDAMESFRKAKQLKRWNG
                     GLSAKYFSKRIRKVFESAG"
     gene            complement(6040..6564)
                     /locus_tag="VNN25_24850"
     CDS             complement(6040..6564)
                     /locus_tag="VNN25_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94827.1"
                     /translation="MAAKKSAKKSKKSAKPAKKSAAKKAAPKKAAPKKAAAKKSSGKK
                     SSGKKASAKKVAAKRPAPKKAAKKAAKKAAAKKVAAKKAAPKRAAAPKKAAAKKAAPK
                     KAAPKKAAPKKKSAPLARAKSVISKVVETVKSLPADIGHAVSNVMPGGNGGSTNEGGS
                     NGGGSSNGGGSSAS"
BASE COUNT         1349 a         2177 c         2048 g         1146 t
ORIGIN      
        1 cggaagaacc tgcccgtggc gacgcttggc gattccgact ctacgcgtgt gagcgagtcg
       61 atctgggcgt tcggctatcc cgccgtggcc agcagcaccg acgacgtcat cggcggctgg
      121 ctttcgcgcg acagcgacct ggagacgacg ctcagtcccg gcatcatcac cgccatcaaa
      181 cgaaacgtcg ccaacacgcc tgtgtttcag tcgaacgtgg ccatctatca gggcaactcg
      241 ggcgggccgg ccgtgaaccg gaacggcgaa gtcgtcggca tctcgacgtg gggccgcagc
      301 gacgctgagg caatcaagtt tctcgttccg atcaacgtcg cccggagttt tcttgccgag
      361 gcgaaggtac cgatcaacct gcagggcgag ttcgacaggc attaccgcgc gtcgctcgac
      421 gccgcgcagg acggcaaatg gaccgtggca tcgagcgagc tggccagcgc gaacagcctc
      481 ttccccaact cgcccgatct gatccacttc cggcgcgata ccgagcgcgc gctgcagtcg
      541 atgccgctct ggcggaagca tcccgtcgcc actgccggcg ccggtggcgc gctcgccgcc
      601 ctcgccctcg gcctgactct tccccgcatc cgtcgcccgc gcatccctgc ggacatgctc
      661 aaagtcacca cagagcgcgt cgtctcgccc tccagcgcgc cgcccggtgc accgcagatg
      721 ctgggcaagc tcacgatcct caacggccct cgcgcgggcg agcgactcgg gctcggcggt
      781 tcgggcattc gcatcggacg ggaatcggcg atgtgtgaga tcgtattgga gaatccgaaa
      841 gtctcccggc tgcatgcgga agtggtctcg atcgatggca aagtgctgtt gatcgaccgg
      901 aactcgtcaa atggcacgta cgtgaacgac cataagatcg acaagaggtt cctgaaagac
      961 ggcgacatca tctatttcgg cggacgcaac gcggttgcga tcgctttcca cgcgtgaggc
     1021 tctgatcgtg gctgataagc ggtgtgagaa cgggcatttc atcgatgaga gctgggatct
     1081 ctgtccgtac tgcccggtgg atgatgccga gccggatgtg ccggtcgtac ggccaacgcg
     1141 gttcggcgca caggatggac agcgtccggc tcccattccc gtcgagccgc cgcgccggcc
     1201 gaccgcggtg cccgcgccga agacgatggc ccgagtcgac gccggtgtcg cgttcgaggc
     1261 cgagccggcg gatcgcaccg tcgccgtgca gaaactcgac ccgaccgcac aaccgaaacg
     1321 ctacgtcgtc gcctggctga tcggactgaa cggcgcggcg cgcggcgaat catttcccgt
     1381 tcgcatcggg cgcaacgtcc tcggccgcga ccggcgctcc gatgtcgtcg ttaacgacga
     1441 tcaggcatcg tcgcatcacg ccgatcttgt ctttcgtccc gaggagcggc ggttcattct
     1501 catggaccac aactcaacca acggtacgta cgtcaacgag gccgagatcg agccgcgccg
     1561 cgaccttgtg gcgaaagaca tcgtgcgcgt cggctcgcac aagttccttt tcgtgcctct
     1621 ttgcgaagag gggttctact gggatgaaga gggtgtcctg aaatgatcgg caacgtcgtc
     1681 ggcaactacc ggatcgagcg cgagctcgga gaaggtggga tgagccacgt ctatgtcggc
     1741 cggaccgtcg cccgcagcga gattctgccc gagggatatg aagtcgtttt gaaggtgatc
     1801 aatgaagagc tggcgggtga agtgacggcc cggaagcgct tcgtcaaaga agcccacatc
     1861 ctcgagaagc tgcggcatcg gtacatcacc cgtttctacg agttcatcaa caaatccgac
     1921 agcgccgtcc tggtgatgga gctcgtcgag ggaactcccg tcgacgtgct gctgaccgag
     1981 cgcggcgcgt tgccgatcgc cgatgccatc ggaacggcgc agtgtctgct cgaagcgctc
     2041 gtctacgcgc acggcaaagg catcgttcac cgcgacatca aaccggccaa cctgatccgc
     2101 gaaaacgcgg gcgtcgtgaa agtgaccgac tttgggattg ccaagatcaa agaaggcggc
     2161 accggcagta cgcagaccgt tctgacgaag tccggcttcc tcctcggcac gccgcactac
     2221 atgtcacccg agcagatccg cgagccgaaa gacgcgggcg ccaagagcga cgtctactca
     2281 accggcgtcg ttctgtacga gatgctgacg ggctcgctac cgttcaacag ccgctcgctg
     2341 ccgaagctga tcgacgctat ctatcgcgga gaaaagcagc caccgcgcgc tctgcggccg
     2401 gagatcgacg ccgagctcga agcgatcgtg ctgcgagcca tgcatccgcg catggagcag
     2461 cgctacgaat cggcgcgtgc atttttcgaa gcgttggagg agtacaacct ccgccccgga
     2521 tacacaccga ccgtacgccc gatgcccgcg gcgcaaatgc ccgccggaaa ggcagccgct
     2581 ccgcaacagt ggcagctcgt gcatgtgaag cccgaagcaa acgaaaagca ctcgctgcag
     2641 ggcgacagca tcatggtcgg ccgcgaccgc acctgttccg tcgtactcac ccatccggcc
     2701 gtctcccgcc ggcatgcccg catcacgatg aacggcgggg cgtgcatgct cgaagatctg
     2761 aagtccgcca atggaacgta tgtcaataac acgcgcgtcg aaaagatcgc gctgaagccg
     2821 ggagacgtcg tgcggttcgg cgccgacccg gcgtgcagtt atgtgttgag gagccaatga
     2881 ttgctgggga gtggggagtg gggcgtaggg agtgggagtc gggtgtcggg tgtagggagt
     2941 cggagaacgc aaagcgggtt cctccgaccc ccgaccccct acacccgact cccacccccc
     3001 actccccaca ccccactccc cacccggaga caccatgagc caaatcaact gccttcgctg
     3061 caaaaagccg atcgatatgg aactggatca gtgtcccgcg tgcggtgagc gcgtgacgat
     3121 gttccagagg acctattcgt cgaagctgct cgacggcaag taccagatcc tcgaccggct
     3181 cggcgtcggc ggcatgggcg agatcttcaa agtccggcac atccacctga acgagctccg
     3241 cgtcatcaag atcatgcggc cgaacgtcgc cgctgacgat cagggcatgc agcgcttcct
     3301 tcaggaggcg cgcacgtcga cgatgatcaa gcacaagaac ctggcgatgc tctacgactt
     3361 cgcccagctc gacgacggct cctactacat ggtgtgggag ttcatcgacg gcaccaacat
     3421 tcagaagtgg attgggcaga acggggcgat gccttcaagg cttgctatcg agatctcgat
     3481 ccaggccctg agcggactcg atcacctcca ctccatgggt ctgatccatc gcgacatttc
     3541 gcccgagaac atcatgctgt cgcaggacca tcacgggaaa ttgctcgtga aggtcatcga
     3601 cttcggcatc gccaagcagc ttgcggaagg cgaaggcgga cagggcctca cgcagaccgg
     3661 catgttcctc ggcaagctga aatacgcctc gcccgagcag gccggcttcc tcaaggaagg
     3721 tgagcatctc gatccacgct ccgacctcta ctcgttcggc atcgtgatgt acgagatgct
     3781 ggccggccgc gcgcccttcc aggccaccaa cccgcacggt tacatcctca aacacgtcac
     3841 cgagaagccc ctgccgattc acgagattaa tccggcagtg aaagtgccgg cgcaggtcga
     3901 agcagtcatc atgcgctcgc tcgaaaagaa ccgcgacaac cgttacgcca cggccgccga
     3961 tttcgagcac gcgctcgaag cgatccgcaa caccgtcccc cccgacgcca agtacgggct
     4021 cggcgaacgg atgatcacac tgtccggcgc acagacgctg gctgagcttc cgaagttcac
     4081 gacgggaccg agcggaacat tcagcggaca gcaagcgacg cagcagacga tgacaggcca
     4141 gccgacgcag gcgacgcagg ccggatcgat gcaagcgccg cgcaccggac cgaccggcac
     4201 gaagatcggc agcgacgaag cgaccgtgat ggagcgcaac ctcgggccgg caaacaccat
     4261 gcccggcgca cctggcacgc gcgccagcag cgacgaagcg acggtgatgg agcgcctcac
     4321 gtcgcaggcg gcaccgaccg cggcggagca gaagtgggca ggcggaaacg ccgaggccac
     4381 gatgatcgag cggcaagtca cgccgggatc gttcgcgccg aaaaagagca acaccggaat
     4441 gatgatcggc atcgccgcgg ccgtcgttat cgttctgggt gttgcgtttt tcgtcatgag
     4501 aaacaaacag agcagcgcgc cgccggccgc ggttccgagc acgaccagcg tcaccgctac
     4561 ggcgccgccg ccatccgcgc cgatcgcggg cgggcaaggc gcgctcctgc tctccgcgtc
     4621 accgtggggc gaactggaag cgatcgtcga cgacaagggc aaaacggtcg acctcaccga
     4681 cgaaaagaag gacatgccga cgcgaatcga tctcgagccg ggccgttaca tggtcacggt
     4741 ggccggcccg aatggaacga agcagaccgt gccggtcgat atcgaagcgg gaaaacggaa
     4801 ccggaaggtg atcaagacgg gtgatgtgaa cttcgacgaa ctggaaaagg aagtcgcgaa
     4861 gccatgaaat ttgcgcgcac cttcaccatc ggattgattg gtttgttgct ggctacggtt
     4921 gcccttgcgt cgtggtacga cgattacgat gcgggcatcg ccgccgtcgg caagggccaa
     4981 tggtccaccg tgattcagaa gatgaccgcg gccatcaatg gtcacccgaa agaaagcgat
     5041 cacgagcgca cctacggcgc gatcttcatc acctatcacc cttattacta tcgcggcgtt
     5101 gcgtatctga atacgggcaa gtacgaacag gcgatctctg atctcgagaa gaccagcgga
     5161 cccggcgaag ccgacctcgg gccgctgggc gacctgctgc ggcgcgcgaa gtcaaaactc
     5221 gaagcggcca gcacgcccga gccgcaaccg cagccccagc cccagcctgt cgcaccgcag
     5281 ccgcgcgttg tgcccacgcc cgtcccgatg ccggtgcaac cggtagctcc atcgatcgat
     5341 cccgcactcc gtcagcaggc agcggcggcg atcaatcagg caaggacgcg cctcgcagcc
     5401 gcgcagcagc ggaaagcggg gaacacaccg cagtacaccc aggccacgca actcctgacc
     5461 gatgccttga cgcgaagcgc caacccgcga tcgaacgacg atctaaccaa tgcgatcggt
     5521 gccgcacaga acgccgcgca gattgccgac ctcgcacccg cccccggcgc tccgattccg
     5581 ccacccgtga ccattcctac gcgtcccgcg caagccagcg cgatcgtact cgccgacccg
     5641 acacgccgcg tccgcgacgc gctggaaagc tatttccgcg gcgagttcga tgacgcatca
     5701 gtgaaattca agacgctctc ccaggagatg cccaagaacg cctggatctg ggccttcctc
     5761 ggcgcctcgc aatactccca atacgccttc gaagccgacg acacctacaa acgcgacgcc
     5821 atggagtcgt tccgcaaagc caagcaactc aagcgctgga acggcggcct ctccgcgaaa
     5881 tacttctcga aacggattcg gaaggtattc gaaagcgcgg ggtagtcgga caaaagaaat
     5941 gcaccacaga gacactgagg acacagagtt gaaaccctac tctgtgccct ctgcgtctct
     6001 gtggtgcaag gggttcttcg atcgcgttcg ttgcggttgc tagctggcgg acgacccgcc
     6061 gccgttgctc gagccgccgc cgtttgatcc gccttcgttg gtggatccgc cgttgccgcc
     6121 gggcatcacg ttcgagacgg catggccgat gtcggccgga aggctcttca cggtctcgac
     6181 gaccttgctg atcacgctct tcgcgcgggc gagcggggcg ctcttcttct tgggcgcggc
     6241 cttcttcgga gcagcctttt tcggggcggc tttcttcgca gcggccttct tcggagcagc
     6301 ggcgcgtttc ggggcggcct tcttcgcggc gacctttttc gcggccgcct tcttcgccgc
     6361 tttcttcgcg gccttcttcg gcgcgggacg cttggcggct actttctttg ccgaggcctt
     6421 cttgccggag gacttcttgc ccgaagactt cttcgcggcg gctttcttcg gagctgcctt
     6481 cttcggtgcg gccttctttg ctgcggactt cttcgcgggt tttgctgact tcttcgactt
     6541 ctttgcagac ttcttggcag ccatggaatc tctcctattc aaaactgtcg ggatggggag
     6601 ccgtacgttt gaatgaaaat ttgtgctgcg tggcaacggc gttgcacaaa tttcaatgta
     6661 gacgaagatg tcgcgattgt cacgcacgca tcagcttccg cgatggctta ctcgatcgat
//
LOCUS       DATLVW010000849         4677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_613431, whole genome shotgun sequence.
ACCESSION   DATLVW010000849 DATLVW010000000
VERSION     DATLVW010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613431"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1538
                     /locus_tag="VNN25_24855"
     CDS             258..1538
                     /locus_tag="VNN25_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94828.1"
                     /translation="MSNRLIDKLLFRDFRIELLESFRIAFSQIRAHKMRSILTALGVI
                     IGIIAVTLMGSAIGGIEIGFERSLAVIGDDILYVEKWPWHNVDDWWNYRNRRRIEPQY
                     ADTLKRLIAATPNSNLITAVPTLNTNRSVKYGDNEVQNVFTQGTTEEFLVTSTATFKE
                     GRFITAIESRGARNVCVLGYDVADALFPKQSALEKTVMIGGSPFRVIGVYGKQGTFLG
                     LFSFDTQVVVPMSAFMKTFSSRLDTSIRVKVKDKEHLSQARDELTGDMRRVRGLRPEQ
                     KDDFEINAQEGLRSTIEPVKAGIAIAGLFVTSLALFVGAIGIMNITYVSVKERTREIG
                     TRKAVGARRRTILMQFLVEAICICLIGGGAGLALSFLMTSGVAAAFPSFPMQFSAGLV
                     IVGMIVSTLTGIFSGFAPALSASRLDPIEALRYE"
     gene            1553..2782
                     /locus_tag="VNN25_24860"
     CDS             1553..2782
                     /locus_tag="VNN25_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94829.1"
                     /translation="MFFTEIFRMALGSLRTNKLRSALTMLGVAIGVFSVIGVMTALSA
                     IQGSIETGLSFLGSNIFQFAKYPVINNGGNAEQKYRNRKDISLDNANEFIRLMGGEAN
                     AIALKVFDGGRPISNGPVTLQGRTVVGTNEYFLTANAYAIAYGRNISPDDVELGRNVT
                     VVGASIQKKLFPSESALGKTVKVNSKPYTIIGVLDEKGSSFGNSQDDLVLVPLTKFFS
                     DFGHENRTINIAIQSTSQATYNDTFDKAVGAMRAARGLKLGQDNDFEIYSNDTLLTAF
                     AQVADTVRVGAFVVSVIALLAAGIGIMNIMLVSVTERTREIGVRKAIGARKFDIIKQF
                     LIEAVLLSELGGLAGIAAGVLGGNGVALAFDMSMVFPWLWAATGLLVCSMIGIGFGSY
                     PAWKAASLHPIEALRYE"
     gene            2847..>4677
                     /locus_tag="VNN25_24865"
     CDS             2847..>4677
                     /locus_tag="VNN25_24865"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HXH94830.1"
                     /translation="MKTAALCLLLCVLIFVPAAYADSITSIAPSAFLQGDIEQFIEID
                     GTGLVGDVDTQVVLSGPFGTTTVAGSPGATDGTVLFVSIPDFVLSVPGTVSVTVDAID
                     SGGTRVIGPGSFDVISNGSSEPPLLSLPESITAEATSASGAIVTFTVSAQSFASGDPL
                     PVTCDHNSGDLYPVAITTVHCSATDAFGTTSGTFEINVSDTVGPVLQLPATIITSNPV
                     VTYTVTATDNTDPSPIVFCTPASGSTFPVGTTTVQCTATDSHANSTHGSFKVSVTGGA
                     TAPTIFVPNDITAEATSGAGAAVSFTVTATDSATILCSPASGSTFPLGTTLVTCSAST
                     AGGTSSDSFHITIVDTTPPSLTLPGTINKGATSPTGAVVTFSATASDLVDGSTPVFCN
                     PPSGSTFPIGTTVVTCSSSDSRFNTTTGTFLVIVTADVTPPVLTLPSNITAEATSASG
                     AVVTYTATAIDDFDGPVPVTCTPASGSTFPLGTTTVQCSAHDAAGNVAHGSFTVTVQD
                     TTPPTIVSITASPNTIWPPNHQMVDVTITVIATDLVDPAPTSHIVSVSSNEADNGNGD
                     GNTTQDFVITGALTLQVRSERDGGNNGSPRIYTITIATSDFSGN"
BASE COUNT          926 a         1568 c         1284 g          899 t
ORIGIN      
        1 acccctcgcc tctgatctct tctgtctccg atctctcaca gcatcatcag agaatagaga
       61 ccatggattc atagactaga gactagagac cagagatcag agaacccctc gtctctgatc
      121 tcttctgtcg ctgatctctc acagcatcat cagaggatag gccatagatt tacagactag
      181 agaccagaga tcagagaacc ctttgtctct gatctcttct gtctctgatc tctgcttcac
      241 aaaccacgag gccacacatg agcaaccgcc tgatcgacaa actccttttc cgcgatttcc
      301 gcatcgagtt gctggagagt ttccggattg cgttcagtca gatccgcgcg cacaagatgc
      361 gctcgatcct cacggccctc ggcgtgatca tcggcatcat cgccgtcacg ctgatgggat
      421 cggccatcgg cggcatcgag atcggattcg agcgcagcct tgccgtcatc ggcgacgaca
      481 tcctttacgt cgagaagtgg ccgtggcaca acgtcgacga ctggtggaac tatcggaacc
      541 gtcgccggat cgagccgcag tacgccgata ccctgaagcg tctcatcgca gcgacgccga
      601 actccaacct catcacagcc gtccccacgc tgaacacgaa tcgcagcgtc aagtacggcg
      661 acaacgaagt gcagaacgtc ttcacgcagg ggacgaccga agagtttctc gtgacgtcga
      721 cggccacgtt caaagagggg cgtttcatca cggccatcga gtcccgcgga gcgcgcaacg
      781 tctgcgtgct cggctacgac gtggccgatg cgctcttccc gaagcagtcc gcactggaga
      841 agacggtgat gatcggcggc agtccgttcc gcgtcatcgg cgtctacgga aaacagggca
      901 ccttcctcgg cctcttcagc ttcgacaccc aggtcgtcgt cccgatgtcc gcattcatga
      961 aaaccttcag ctcgcgcctc gatacctcga tccgcgtcaa ggtcaaagac aaagaacatc
     1021 tttcccaggc gcgcgatgag ctgaccggcg acatgcgccg cgtccgcggt ctgcggccgg
     1081 agcagaagga cgacttcgag atcaacgcgc aggaaggtct gcgcagcacg atcgagccgg
     1141 tcaaagccgg catcgccatc gccggcctgt tcgtcaccag cctggcgctg ttcgtcggcg
     1201 ccatcggcat catgaacatc acctatgtga gcgtgaagga acggactcgc gagatcggta
     1261 cgcgcaaagc cgtcggcgcg cggcggcgaa cgatcctgat gcagttcctc gtcgaggcca
     1321 tctgcatctg cctgatcggc ggaggcgcgg ggttggcgct ctccttcctg atgacctccg
     1381 gcgtcgcggc agctttccca tcattcccca tgcagttttc cgctggactc gtgatcgtcg
     1441 ggatgatcgt ctcgacgctc accggcattt tctccggctt cgcgccggcg ttgtcagcgt
     1501 cgcgattgga tccgattgaa gcattgaggt acgagtagtt cactcacaca ccatgttctt
     1561 caccgaaatc tttcgaatgg ccctcggttc gttgcggacg aacaagcttc gctccgcgct
     1621 gacgatgctc ggcgtggcca tcggcgtctt ttccgtcatc ggcgtcatga cggcgctcag
     1681 cgccattcag ggctccatcg aaacgggcct cagcttcctc ggcagcaaca tcttccagtt
     1741 cgccaagtat ccggtcatca acaacggagg caatgccgaa cagaagtacc ggaaccggaa
     1801 agacatctcg ctcgacaacg ccaacgagtt cattcgactg atgggcggcg aggcgaacgc
     1861 catcgctctc aaggtgttcg acggcggccg gccgatcagc aacggcccag tgacgttgca
     1921 gggacgcacc gtcgtcggca ccaacgagta cttccttacc gccaacgcct acgccatcgc
     1981 ttacggccgc aacatctcgc ccgacgatgt ggagctcggc cgcaacgtga ccgtggtcgg
     2041 cgcgagcatc cagaagaagc tcttcccgag cgagtccgct cttggcaaga cggtcaaggt
     2101 caactccaaa ccgtacacga tcatcggcgt gctggatgag aagggctcgt cgttcgggaa
     2161 cagtcaggat gacctcgtgc tcgtgccgct cacgaaattc ttttccgact tcggccacga
     2221 gaaccgcacc atcaacatcg ccatccagtc gacgtcgcag gctacctaca acgacacgtt
     2281 cgacaaagcg gtcggagcga tgcgcgccgc ccgcggtttg aagctcgggc aggacaacga
     2341 cttcgaaatt tactcaaatg acacattgct cactgccttc gcacaggtgg ccgacacggt
     2401 gcgcgtcggc gcgttcgtcg tcagcgtcat cgcccttctg gccgccggca tcggcatcat
     2461 gaacatcatg ctcgtcagcg tcaccgagcg gacgcgtgag atcggcgtgc gcaaagcgat
     2521 cggcgcacga aagttcgaca tcatcaaaca gttcctcatc gaggccgttc ttctctcgga
     2581 actgggcgga ttggccggaa ttgcggccgg tgtcctcggc ggcaacggcg tcgcccttgc
     2641 cttcgacatg agcatggttt ttccgtggct ctgggcggca accggccttc tcgtttgctc
     2701 gatgatcggc atcggtttcg gctcttatcc ggcctggaaa gctgcctcat tgcacccgat
     2761 tgaagcgctt cgctacgaat aactcattcc atttacacac ggcgaaaagc ggagcataat
     2821 agtcactccg aaccggagga ttaagcatga aaactgcggc cctttgcctg ctgctgtgtg
     2881 ttctcatttt cgtacctgcg gcctacgctg attcaatcac ctcgatcgcg ccgtcggcat
     2941 tccttcaagg cgacatcgaa caattcatcg agatcgatgg taccggcctt gttggggacg
     3001 tcgatactca ggtcgtcctg tcgggacctt tcggcacgac caccgttgcg ggtagtcccg
     3061 gcgctaccga cggaacggtc ctcttcgtga gcatccctga tttcgtgctt tcggttcccg
     3121 gcacggtctc cgtgacggtc gacgcgatcg acagcggcgg cacgcgcgta atcggtcccg
     3181 gatctttcga cgtgatctcg aacggctcgt cggagccgcc gctgctatcg cttccggagt
     3241 ccatcaccgc cgaggcgacc agcgcgtccg gagcgatcgt caccttcacc gtcagcgcgc
     3301 aaagcttcgc ttccggtgat ccactgccgg tcacctgcga tcacaattcc ggcgatcttt
     3361 atcctgtcgc catcaccacg gtccactgct cggcgacgga cgccttcggc acgacatcag
     3421 gaaccttcga gatcaacgtc agcgacaccg tggggccggt cctgcagctt ccggcaacga
     3481 tcatcaccag caacccggtc gtgacgtata cggtgaccgc gactgacaat accgatccta
     3541 gtcccatcgt tttctgcact cccgcatcgg gctcgacgtt ccccgtcgga acgacgaccg
     3601 ttcagtgcac cgccaccgac tcgcacgcca acagcacgca cggctcgttc aaggtcagtg
     3661 tcacgggcgg agccacagca cccaccatct ttgtgccgaa cgacattacg gccgaggcaa
     3721 ccagtggagc cggagcagcc gtgtcgttca ccgtcaccgc gaccgacagc gccacgatcc
     3781 tctgcagccc cgcttccgga tcgaccttcc cgctcgggac gacgttggtt acctgctcgg
     3841 catcgacggc aggtggaact tcttccgaca gcttccacat cacgatcgtc gatacgacgc
     3901 cgccgtcgtt gacgctcccc ggtacgatca acaagggcgc caccagcccg accggagccg
     3961 tcgtcacctt ctccgccacc gccagcgatc tcgtcgatgg aagcaccccc gtcttctgca
     4021 atccgccttc gggctccacg ttcccgatcg gaacgacggt cgtcacgtgc agttcgtcgg
     4081 acagccggtt caacaccacg accggaacct tcctggtcat cgtgacggcg gacgtcacgc
     4141 cgcctgtcct tacgctgccg tcgaacatca ccgccgaagc aaccagcgct tccggcgccg
     4201 tcgtcaccta caccgcaaca gccatcgacg acttcgacgg accggttcct gtgacttgca
     4261 ccccggcgtc gggatcgacg ttcccgctcg gcacgacgac cgtacagtgc agcgcgcacg
     4321 atgcggccgg caacgtcgca cacggctcct tcacggtcac cgtgcaggac accacaccgc
     4381 cgacgatcgt ctcgatcacg gcatcgccga acacgatctg gccgccgaat catcagatgg
     4441 tcgacgtgac gatcactgtg attgcgaccg acctggtcga tccggcgccg acgtcgcaca
     4501 tcgtctcggt gtcgtcgaac gaagccgaca atggcaacgg cgacggaaat acgacgcagg
     4561 acttcgtgat caccggcgcg ctgacgctgc aggtccgctc cgagcgcgac ggcggcaaca
     4621 atggcagccc ccgcatctac acgatcacga tcgcgaccag cgacttcagc ggcaaca
//
LOCUS       DATLVW010000850         4800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_571337, whole genome shotgun sequence.
ACCESSION   DATLVW010000850 DATLVW010000000
VERSION     DATLVW010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571337"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /gene="murD"
                     /locus_tag="VNN25_24870"
     CDS             complement(<1..1300)
                     /gene="murD"
                     /locus_tag="VNN25_24870"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXH94831.1"
                     /translation="MTRTLIIGAGKSGVAAANFLAARGEDVSITDSKTDPELPYPLDS
                     SVKRVFGRDDGGVLDGIGMIVLSPGVPSTIPLLSRTGSIPVVGEIELAYRYVPRGSRI
                     VAVTGSNGKSTTTMLIGEILKVAGLQPIVAGNIGEPFIAAVDLEKPRMYVLELSSFQL
                     ESVDTFHANVALLLNVTPDHMDRYPSFDAYAAAKYRIFRNQQPHDVAVVNAADRRAQP
                     RDSVSRLWRFSSTQVIEEGAFLDGGDLVLRVDGQERRIPRASLKLEGTANAENALAAW
                     LAVKALGVDEVSVQIAFGSFAGLPHRMVLVRELDGVRWINDSKGTNVDATLKSLESFP
                     ASSVWLILGGKDKAGEFERMRDLVKEKTRAVLTIGAAADRVAAALDGAATIVSAGDMQ
                     HAVAYAREHAKSGDVVLLSPACASFDQFRNFEHRGEHFEEL"
     gene            complement(1535..2623)
                     /gene="mraY"
                     /locus_tag="VNN25_24875"
     CDS             complement(1535..2623)
                     /gene="mraY"
                     /locus_tag="VNN25_24875"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXH94832.1"
                     /translation="MLYYLLYPLRNMISGFNVFRYITFRSAWAALTALIISFIFGPWL
                     IERMRRIKLGQYIREEGPKSHQQKAGTPTMGGILINVAIIIPTILWADILNPYIWIIL
                     FVTFAYGVIGFVDDYRKLIKKKNEGLTPREKFSMQIGVALAAGLAIAYLPSIHNNYST
                     VLTFPFFKMLHLNLGFLYIPFIIVVLVGASNGVNLTDGLDGLAIGSTLIVAVTYTILT
                     YAAGNFRVADYLRIAWVPQAAELAVFCGAMVGASLGFLWFNAHPAEIFMGDVGSLALG
                     GAIGCLAVMIKQEILLGLVGGLFVLEGMSVVLQVASFKLTGRRIFKMSPLHHHFELSG
                     WRETKVVVRFWIIAIIFALISLSTLKLR"
     gene            complement(2623..3999)
                     /gene="murF"
                     /locus_tag="VNN25_24880"
     CDS             complement(2623..3999)
                     /gene="murF"
                     /locus_tag="VNN25_24880"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXH94833.1"
                     /translation="MHLTFQQIADMTGGQVAQGGDVAIDSVVIDSREVKADSVFFAIT
                     GEHLDGHQFLAQALTTARGAVVSNVPESTPADKGIVRVADTTVALQNLARSIRERYPF
                     TLIAITGSAGKTTTKEMIATLVASERRTFKSWGNFNNLIGCPLCIDNTPDDAEVVVSE
                     MGMNHKGEIALLAGLLHPDVGVYTNIGPVHIEFFGTIEKIAEAKRELLENVKPGGTII
                     LNADNPYVMDISRGFEGRKMTYGIDHEADFRGVNIRERGLLGTSFEVAGHSFSLALPG
                     RHNLENLLAAIAAARSIGISWEGIERGVGNVKPAAHRGIILDAGGATIYDDTYNSNPY
                     ALGRALTLMEQADVTGRRIAVIGDMLELGEKELDYHRDAGRAIPRSIDVVIGVGKRSH
                     ALLEGAAEAGFRNGDRHHFDDAQSAAEFLKTFIRPGDLVLIKASRGIGLDRTVNLLTN
                     SGTAEGAH"
     gene            complement(4316..>4800)
                     /locus_tag="VNN25_24885"
     CDS             complement(4316..>4800)
                     /locus_tag="VNN25_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXH94834.1"
                     /translation="GPTVIVDYAHKPDALEKLLHAVRDLARGRRVVVIVGCGGDRDKD
                     KRGKMGAIAARLADRVVLTSDNPRSEKPEAILDGIEAGMKGASNYERVTDRREAIART
                     IAGASDNDVIVIAGKGHESYQVIGDQVIHFDDREEAERALQKQPKTRHKNEAVKSISN
                     "
BASE COUNT          925 a         1461 c         1463 g          951 t
ORIGIN      
        1 ctaattcttc aaagtgctcg ccacgatgtt cgaagtttcg gaactgatca aagctggcgc
       61 aggcgggcga gaggaggacg acatcgccgg acttcgcatg ttcgcgcgcg taggcgacgg
      121 catgttgcat gtcgcctgcg ctcacgatcg tggcggcccc gtcgagtgcg gcggcgacgc
      181 ggtcggcggc ggcaccgatc gtgaggaccg cgcgggtctt ttccttgacg agatcgcgca
      241 tccgctcgaa ctcacccgct ttgtctttgc ctccaaggat cagccagacg ctcgacgccg
      301 gaaacgattc gagcgacttg agcgtggcat cgacgttcgt tcccttcgag tcattgatcc
      361 agcggacacc gtccagctcg cgcacgagaa ccatgcgatg cggcagaccg gcaaacgatc
      421 cgaacgcgat ctgcacgctg acctcatcga cgccgagcgc cttgacggcc agccacgcgg
      481 caagcgcgtt ctcggcattc gcggttcctt ccaacttcag cgaggcgcgc ggaatgcggc
      541 gctcctggcc atcgacgcgc aacacgaggt cgccgccatc gagaaaggca ccctcctcga
      601 tcacttgcgt ggatgagaaa cgccaaaggc ggctgacgga gtcgcgaggc tgcgcacggc
      661 ggtcggcagc attgacgacg gcgacatcgt gcggctgctg attgcggaag atccggtact
      721 tcgccgcggc gtacgcgtcg aaactcgggt agcggtccat gtgatcgggc gtgacgttga
      781 ggagcaacgc cacgtttgcg tggaaggtgt cgacactctc gagctgaaac gacgacagct
      841 cgagcacata cattctcggc ttctccaagt cgaccgcggc aatgaacggc tcgccaatgt
      901 tgccggcaac gatcggctgc agtccggcca ctttgagaat ctctccgatc agcatcgtcg
      961 tcgtcgattt cccattcgaa ccggtcaccg cgacgatcct gcttccgcgg ggcacatacc
     1021 gataggcaag ctcgatctca ccaaccacag gaatcgagcc ggtccgcgac aacagcggaa
     1081 tcgtcgatgg cacaccagga ctcagaacga tcatcccgat tccgtcgagc acaccaccgt
     1141 cgtcgcggcc gaacacgcgc ttgaccgacg agtccagcgg atacggcagc tcgggatcgg
     1201 tcttcgagtc ggtgatcgac acatcttcgc cgcgcgcagc tagaaaattc gctgcggcca
     1261 cgcctgattt gcctgctccg atgatgaggg ttctggtcat gtgttcggtt tttgttttcg
     1321 agagatcaga gactagagat tagagatcag gaaaaaacct gccgcgatgc tctgatctct
     1381 aatctcttgt ctctgctttc tgtttcgaga gatcagagac gagagatcag agatcagaag
     1441 aaacctgccg tgatcccctg atctctgatc tctcatccct tgtctctgct tcttcccccg
     1501 atctctgatc tctaatccct tgtctctgct tttctcacct caacttcaac gtcgacagcg
     1561 agatcagcgc gaagatgatg gcgatgatcc agaagcggac cacgaccttc gtctcgcgcc
     1621 atcccgaaag ctcgaaatgg tgatgcagcg gcgacatctt gaaaatgcgg cgcccggtca
     1681 atttgaatga ggccacctgc aggacgacgg acattccttc cagcacgaag agaccgccga
     1741 cgaggccgag gaggatttcc tgtttgatca tcacggccag gcagccgatg gcaccaccca
     1801 acgcgagcga gccaacatca cccatgaaaa tctccgcggg atgggcgttg aaccataaaa
     1861 atccgagtga cgccccgacc atcgccccgc agaacacggc cagctcggcg gcctgcggaa
     1921 cccaggcgat gcgcaggtaa tcggcgacgc ggaagttgcc ggcggcgtac gtgaggatcg
     1981 tgtaggtcac ggccacgatc agcgtcgatc cgatggccaa tccatccagg ccgtcggtga
     2041 gattcacgcc gttcgatgcg ccgacgagga cgacgatgat gaatgggatg tagagaaacc
     2101 caaggttcag gtgaagcatc ttgaagaacg ggaaggtgag gacagtcgag tagttgttgt
     2161 gaatcgacgg caggtaggcg atggccagcc ccgcggccag cgccacaccg atctgcatcg
     2221 agaacttttc tctcggcgtc agaccctcgt tcttcttctt gatcagcttg cggtagtcat
     2281 cgacgaatcc gatcacgccg tacgcgaagg tgacgaagag gatgatccag atgtagggat
     2341 tgagaatgtc ggcccagaga atcgtcggaa tgatgatggc gacgttgatg aggatgccgc
     2401 ccatcgtcgg cgtgccggcc ttctgctggt gcgacttcgg cccttcttcg cggatgtact
     2461 ggccgagctt gatgcgccgc atccgctcga tcagccacgg gccgaagatg aacgaaatga
     2521 tcagcgccgt gagcgccgcc cacgccgacc ggaacgtgat gtagcggaag acgttgaagc
     2581 cggaaatcat gttccggagc gggtagagca ggtagtacaa catcagtgcg cgccctccgc
     2641 ggtgcccgag ttggttagca ggttgacggt gcggtcgagg ccgatcccgc ggctggcttt
     2701 gatcagcacg aggtcccccg gacggatgaa agtcttcaga aactcggcgg cgctctgcgc
     2761 gtcatcgaag tgatggcggt ctccattgcg gaaccccgcc tcggcggccc cttcgagcag
     2821 agcatgcgaa cgtttgccga cgccgatcac gacgtcgatg cttctgggga tggctcgtcc
     2881 ggcatcacgg tggtagtcga gctctttctc gcccagctcg agcatgtcgc cgatcacggc
     2941 gatacggcgg ccggtgacgt cggcctgttc catcaaggtc agggcgcggc cgagggcgta
     3001 ggggttcgag ttgtaggtgt cgtcgtagat cgtcgcgccg ccggcgtcga ggatgatgcc
     3061 gcgatgcgca gccggcttca cgttaccgac gccgcgctcg atcccttccc acgagatgcc
     3121 gatggatcgc gcggctgcga tggcggcaag gagattctcc agattgtgcc gccctggaag
     3181 tgccagagaa aacgaatggc ccgcgacttc gaacgacgtt cccagaaggc cgcgctcgcg
     3241 gatgttcaca ccgcggaagt cggcttcgtg atcgatgcca tacgtcatct tgcggccttc
     3301 gaaaccgcgg ctgatgtcca tcacgtacgg attgtcggcg ttgagaatga tcgttccgcc
     3361 gggcttcacg ttctcgagca gctcgcgctt cgcttcggcg atcttctcga tcgtgccgaa
     3421 gaactcgatg tgtactggcc cgatgttcgt gtagacgccg acgtcgggat ggagaaggcc
     3481 ggccagcagc gcgatctctc ctttgtgatt catccccatc tcgctgacga cgacttcggc
     3541 atcgtcgggc gtgttgtcga tgcacagcgg gcatccgatc aggttgttga agttgcccca
     3601 cgacttgaac gtgcggcgtt cggaggcgac gagcgtggcg atcatctcct tcgtcgtcgt
     3661 ctttcctgcc gaaccggtga tggcgatgag cgtgaacgga taacgttcgc ggatggaccg
     3721 cgcaaggttc tgcaacgcga ccgtcgtatc ggcaacgcgc acgattcctt tatccgcggg
     3781 cgtgctctca ggaacattcg acacgaccgc accgcgcgcg gtggtcagcg cttgcgcaag
     3841 aaactgatga ccgtccagat gctcgccagt gatggcgaag aagacggagt cggccttcac
     3901 ttcgcggctg tcgatgacga cagagtcgat ggcgacgtcg ccgccctgcg cgacctggcc
     3961 accagtcatg tccgcaatct gctggaaggt caggtgcatg cggcctcccg ggagtggtgt
     4021 gtttgggggg tgcactccaa gtcccagtca cgggattcac cacagaggca cagagaacac
     4081 agagggtaca aaccaaactc tctgtgttct ctgtgtctct gtggtgaatc ccgtcggacc
     4141 cccgcgagat cgctctcttc cggctccgcc gcgccaaccc gatcgcggcc cttcccccga
     4201 agctgcccgg cgatcgccaa ccatccaccc gcacagaagg caatcgacgg agccggaaca
     4261 gagcacctgt ctgacgtcgc taaataataa gacgaattga tcattgattt caacactaat
     4321 ttgatatcga tttaactgct tcgtttttgt ggcgtgtttt cggttgcttc tgcaatgcgc
     4381 gctccgcttc ttcgcggtcg tcgaagtgga tgacctgatc gccgatgacc tggtacgact
     4441 cgtggccctt gcctgcgatg acgatgacgt cgttgtcgga tgcgcccgcg atcgtccgcg
     4501 cgatggcctc gcgccggtcg gtgacgcgct cgtagttcga cgcgcccttc attcccgcct
     4561 cgattccgtc gagaatcgct tccggctttt cgctgcgcgg gttgtcgctt gtcaggacga
     4621 cgcggtccgc gagacgcgcc gcgatcgctc ccatctttcc gcgcttgtct ttgtcgcggt
     4681 cgccgccgca gccgacgatc acgacgacgc gccggccgcg ggcgagatcg cgaacggcgt
     4741 gcagcaactt ctccagagcg tcgggtttgt gggcgtagtc gacgatcacc gtcgggccgc
//
LOCUS       DATLVW010000851         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_488644, whole genome shotgun sequence.
ACCESSION   DATLVW010000851 DATLVW010000000
VERSION     DATLVW010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488644"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /locus_tag="VNN25_24890"
     CDS             <1..1612
                     /locus_tag="VNN25_24890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94835.1"
                     /translation="DLVPPAGIQQVPSIDVRNFALSPDGQTLCFTGVQNGAQSIFVRR
                     LDSLTVRKIEGSENGSGPFWSSDGKWIGFSARSKLWKTQAANNAVPEALCDVSPAGAT
                     ASWVGSAILFYGGPGGPPEIFRISDQGGTPVKVTSLKAGQSWHGWPRLLPDGRRFLYV
                     ATWGKSNRELVLASLDSSSSSSALLRNVSEVALLGPGDLLYVRDGKLFSQKYDPDRAA
                     MAGEPSLIADDISYFYPSAEAEFDAANGVVVYRTDTTTGSLLKTDRTGKARLIDDHGP
                     FDPFSLSISPDGKRATATIIKNHSTQIGDIWIYDLARGTRDRFTDEGGLALDPVWSPD
                     GRSIVYSSIEGEGPHLVRRSLTGSTREELMPRGNFQFAGSFSSDGGTLYFRQFPWARP
                     EVFRLDMRTRKAEPVLSSFFGRDPMVSPAGRWLAFSSDATSSSEVYLQSLTDKDMPRQ
                     RISTNGGQNPCWRRDGGELFYLSQNAVMSVVPRVAGDWSDTTSTELLHAPADTVRFAA
                     SPDGQSFLFITGKPGAEDSLFHVILGLH"
     gene            1718..>2805
                     /locus_tag="VNN25_24895"
     CDS             1718..>2805
                     /locus_tag="VNN25_24895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH94836.1"
                     /translation="MIAAHVSLAKGTMLGPYEVDILLGVGGMGEVYRARDTRLGRTVA
                     IKVLLAGLTASSERRARFEREARVISALSDPHICALYDIGRDGDTEYLVMEYIEGETL
                     ADRIARGRLPLSQVLRFGTEIAQALQHAHRAGITHRDLKPGNVMLTTSGVKLLDFGLA
                     KLAIPDPAPGDPDAATLVNPITAEGMVVGTLVYMSPEQLEGRNIDHRTDIFALGCILY
                     EMATGQRPFGGSSRIAISSAILSSDPAPIRSLEPATPPALERIILTALEKNPEDRWQT
                     AQDVARQLRWLSETSSATEPVSSIKSSHLSRRLLPALMIAAAIGGLLAWGGTRLFTAR
                     PASPAAVHLQLALPEGVHGYLTSETPNLA"
BASE COUNT          533 a          873 c          832 g          567 t
ORIGIN      
        1 cgatctcgtt cctccggctg gcattcaaca agtgccatcg atcgacgtgc gcaacttcgc
       61 gctctcaccc gatggccaga cactctgttt cacgggagtg cagaacggcg cgcagtcgat
      121 cttcgtcagg cgtctcgact cgttgactgt aagaaagatc gaaggctcgg agaacgggtc
      181 gggccccttc tggtcctcgg acggaaaatg gatcggattc agcgcgcgca gcaaactttg
      241 gaagacacag gccgcaaaca atgcggtccc cgaggcgctg tgcgatgtct cgccggcagg
      301 tgcgacggca tcgtgggtag gaagcgccat cctcttttac ggagggccag gtggaccacc
      361 ggagattttc cggatctccg accagggcgg aacgccggtg aaagtgacct ctctgaaagc
      421 aggccagtcg tggcatgggt ggcctcgact attgcccgac gggcgacgct ttctctatgt
      481 ggcaacttgg ggaaaatcga atcgggaact ggtcctggca tctctcgact cgtcgtcttc
      541 ctcttcggcg ctcctccgaa acgtttcgga ggtggctctt ctgggtcccg gcgatctgct
      601 ttacgttcgt gacggtaaac tcttttcaca gaagtacgat ccggataggg cggcaatggc
      661 tggagagcct tcattgatcg cggatgacat ctcgtatttc tacccgtccg ccgaggccga
      721 gttcgatgca gcaaacggcg tcgttgtcta tcgcacagac acgactaccg gcagcctttt
      781 gaagaccgat cgaacgggaa aggcgcgcct catcgatgat catggaccgt tcgatccgtt
      841 tagcctgagc atctcgccgg atggcaagcg ggcgacagcc acaataatca aaaaccactc
      901 aacgcagatc ggggacatct ggatctacga tcttgcccgt gggacaaggg accgattcac
      961 tgatgaaggc ggcttggcgc tcgatcctgt ttggtcaccc gatggaaggt cgatcgttta
     1021 ctcgtcgatc gaaggagaag gcccgcatct tgttcgccgc tcccttactg gatcaacgcg
     1081 cgaggagcta atgccacggg gaaactttca attcgccgga agcttttcgt ccgatggcgg
     1141 cactctctac ttcaggcagt tcccgtgggc cagaccggaa gtattccgtc tcgacatgcg
     1201 aacccgaaag gccgagccag tgctctccag ctttttcgga cgcgatccga tggtttcgcc
     1261 tgccggacgt tggctggcct tttcttcgga tgcaacaagc tcctccgaag tttatctaca
     1321 gagtttgacc gacaaagaca tgccccgaca acggatctcg acgaatggcg gccagaatcc
     1381 ttgttggcgc cgtgatggcg gcgagctgtt ctatctctcc cagaatgccg tgatgagcgt
     1441 ggttccacgc gtcgccggag actggagcga taccaccagc acggaacttc tccacgcgcc
     1501 tgcggacaca gtccgttttg cagcgagccc ggacggccag tcatttctgt tcatcacagg
     1561 caagccgggc gcagaggact cgctcttcca cgtgattctc ggtttgcact gagttactgc
     1621 acgcgagact cgctggagcc gtttcacgct gagacacgga ggacgcggag tactccgcga
     1681 ctccgcgtct ccgcgtgacc gcttttgatt ctccgctatg atcgccgcac acgtgagtct
     1741 ggcgaaggga acgatgcttg gcccctacga ggtggacatt ctcctcgggg tcggagggat
     1801 gggcgaggtt tatcgggccc gcgacacgcg cctcgggcgc accgtcgcca tcaaggttct
     1861 cctcgccgga ctgaccgcaa gcagcgagcg gcgtgcacga ttcgagcgtg aggcgcgcgt
     1921 catctccgca ctctccgatc cccatatctg tgcgctctac gacatcggcc gcgacggcga
     1981 caccgaatac ctggtgatgg agtacatcga aggggaaacg ctcgccgatc ggattgcgcg
     2041 cggacgcctt ccgctcagtc aggttctgcg attcggcact gagatcgccc aggcgctgca
     2101 gcatgcgcat cgtgccggca tcacgcatcg cgatctcaag ccgggcaacg tcatgctcac
     2161 cacctcggga gtcaagcttc tcgacttcgg tctcgccaag ctggccattc ccgaccctgc
     2221 gcccggcgac cccgacgccg cgacgttggt caatccgatc actgcggaag ggatggtcgt
     2281 cggcacgctc gtctacatgt cgccggagca gctcgaaggg cggaacatcg atcaccgcac
     2341 cgacatcttc gcgctgggct gcatcctgta cgagatggcc accgggcagc ggccgtttgg
     2401 aggcagctca cgcatcgcca tcagcagcgc gattctctcc tccgatccgg ctccgatccg
     2461 ctcgctcgaa ccggccactc ctccggctct cgaacggatc attctcaccg ctctggaaaa
     2521 gaatcccgag gatcgatggc agacggcgca ggacgtcgcg cgccagttgc gatggctgtc
     2581 ggaaacttcg tcggcgacgg aaccggtctc atcgatcaag tcctcgcatc tcagccgtcg
     2641 cttgttgcct gccttgatga tcgcagcggc aattggcggg ctgctggcgt ggggaggaac
     2701 gcggttgttt acagcccgcc cagccagtcc tgccgcggtt catttgcaac tcgcgctccc
     2761 cgaaggagtt cacgggtacc tgacgtcgga gacaccaaac ctcgc
//
LOCUS       DATLVW010000852         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_118610, whole genome shotgun sequence.
ACCESSION   DATLVW010000852 DATLVW010000000
VERSION     DATLVW010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118610"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VNN25_24900"
     CDS             complement(<1..504)
                     /locus_tag="VNN25_24900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94837.1"
                     /translation="MRRSVSCFALLIALVATLPAAAQSQPPQTLPEVRDLTPPRPQDP
                     THDITMVDTAPLGGAIAIPLPERERRKQAKLDVPELAGCRQALGTQRIDGRLPRPLVD
                     FNINVAAVYQRLSIFEGGLVVVEVRGTGGTIRKKVLLPPDALQNYLHVISPQALALVR
                     DGDLTKPR"
     gene            complement(540..1808)
                     /locus_tag="VNN25_24905"
     CDS             complement(540..1808)
                     /locus_tag="VNN25_24905"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94838.1"
                     /translation="MTRLENLAPLQSRALNLVREVATEKELRPFLVGGPVRDLILGRD
                     NVVDVDLTLEEGGSTLARALAKQVGGRLRSFPQFLTYKVTAEGLPEIDIATARKERYR
                     SPGALPTVTAGRLKDDLIRRDFSINALAIDVMSGEMLDPTGGARDLEARIIRVLHDES
                     FADDPTRIFRAIRLCIRLGFTLEEHSADLMRAALENGALSTIAKERVWRELFLAFDEE
                     DAPSTVAALNDAGALDVLFGRRPDDPKLPSRLQRARAAIEAAVTQADLDREVMYTGAI
                     LYGNASPVDLEGSGFSQKRARNLIQIANELPRFTDALAEATSDHQRFRLFKHASPEML
                     SIVAASAPDEQPHVARFLEFRSFKLPLRGNDLEVPLGPHVARALERTREAVFTGEIEQ
                     TDARTFARAMAIKYLNGSAQRPEGSGQEPV"
     gene            complement(1805..>2237)
                     /locus_tag="VNN25_24910"
     CDS             complement(1805..>2237)
                     /locus_tag="VNN25_24910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94839.1"
                     /translation="LSRPSIRNIVEQTATRIHRFREDVDWLLFDEQPSLGDLHSVDIW
                     VDPAVEEDDADGFVAEAMAAGKAVVASRTAINTQRLEKGRTGILVPPHDPNELTHAIL
                     AALFKSELAQDIVTASQQTVSKFRARHRVRALVQLYESILQ"
BASE COUNT          376 a          684 c          776 g          401 t
ORIGIN      
        1 ccgcggtttc gtcaggtcac catcgcgcac caaggcgaga gcttgcggcg aaatgacgtg
       61 caggtagttc tgcagagcat cgggaggtaa caataccttc ttgcggatcg tgcctccggt
      121 gccgcggacc tccacgacga ccagcccccc ttcgaagatc gaaagacgct ggtagacggc
      181 cgcgacgttg atgttgaagt cgaccagcgg cctgggaagc ctgccgtcga tccgttgcgt
      241 gccgagggcc tgccggcagc cggcgagctc cgggacgtcg agtttcgcct gctttctccg
      301 ctcgcgctcg ggaagcggga tggcgatggc gccgccgagg ggagcggtat cgaccatggt
      361 gatgtcgtgc gtcggatcct gaggccgagg cggcgtcagg tcgcgcactt ccggcagcgt
      421 ttgcggaggc tgggattgcg cggcggctgg cagggtcgcg acgagcgcga tcaggagcgc
      481 aaagcaggaa acggagcgcc gcatacggtt gtgagtcgcc agaagccggt cgtcagcggt
      541 cagacgggtt cctggccgct gccttccggc cgctgcgcac tgccgttaag gtacttgatc
      601 gccatcgccc gcgcaaacgt gcgcgcgtcc gtctgctcga tctcgccggt aaatacggcc
      661 tcgcgcgtcc tctccagagc gcgggccacg tggggaccga gcggcacttc gaggtcattg
      721 ccgcgcagcg gaagcttgaa tgagcgaaac tccaggaagc gggccacgtg gggctgctcg
      781 tcgggtgccg aggcggcgac gatgctgagc atctccggcg aggcgtgctt gaacagccgg
      841 aacctctgat ggtccgacgt cgcctcggcc agcgcatccg tgaagcgcgg caactcgttc
      901 gcgatctgga tgagattgcg ggcccgtttc tgcgaaaagc ccgagccctc gaggtccacc
      961 ggtgacgcgt ttccgtagag gatcgctccc gtatacatca cctcgcggtc gagatcggcc
     1021 tgcgtgaccg ctgcctcgat cgccgcgcgg gcgcgctgca ggcgggaagg cagcttggga
     1081 tcgtcgggcc gccggccgaa cagcacatcg agcgcgccgg catcgttcaa cgcggcaacg
     1141 gttgacgggg catcctcctc gtcgaacgcc aggaagagct cgcgccacac acgctccttc
     1201 gcaatcgtag agagggcgcc gttctcgagt gccgcccgca tcaggtccgc cgagtgttct
     1261 tccagcgtga agccgagccg gatgcagaga cggatcgcgc gaaagatgcg cgtggggtcg
     1321 tcggcgaaac tctcgtcgtg caacacgcga atgatgcggg cttccaggtc gcgcgcgccg
     1381 ccggtcggat cgagcatctc gccgctcatg acgtcgatgg ccagggcgtt gatcgagaag
     1441 tcgcgccgga tgagatcgtc cttcagcctg ccggcggtga ccgtgggtaa cgcgcccggc
     1501 gagcggtaac gttccttgcg ggcagtggcg atgtcgatct ccggcagccc ttccgcggtg
     1561 actttgtagg tcaggaactg aggaaaggat cgcagccggc cacccacttg tttggccagg
     1621 gcacgcgcca gcgtcgatcc cccttcctcg agcgtgagat cgacgtcgac gacgttgtcc
     1681 ctgccgagga tgagatcgcg gacaggaccg ccgaccagga acgggcggag ctccttttcg
     1741 gtggccactt cccttaccag attgagggcg cggctttgga gaggcgccag gttttcgaga
     1801 cgggtcattg gagaatggat tcgtagagtt ggactagcgc ccgaacccgg tgccgggcgc
     1861 ggaactttga gaccgtttgt tgagatgccg taactatatc ctgtgcaagc tcggacttga
     1921 aaagggcggc aagtatcgca tgggtcaatt cgttcggatc gtgcggtggg acaaggatgc
     1981 ctgtgcgccc cttctccagc cgttgtgtgt tgatcgctgt tcgcgaggcc acgaccgcct
     2041 ttcccgccgc catcgcctcg gcgacaaagc cgtcggcgtc gtcctcctct acggcgggat
     2101 cgacccagat gtccacgctg tgtagatccc cgagggacgg ctgctcgtcg aaaaggagcc
     2161 agtcgacgtc ttcgcggaac cggtggattc gcgtagcggt ctgttcgacg atgttgcgga
     2221 tcgaggggcg ggataag
//
LOCUS       DATLVW010000853         4653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_324558, whole genome shotgun sequence.
ACCESSION   DATLVW010000853 DATLVW010000000
VERSION     DATLVW010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324558"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VNN25_24915"
     CDS             complement(<1..1296)
                     /locus_tag="VNN25_24915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94840.1"
                     /translation="MPPTTPDPRGKDDTQHPVDDITTQPRASLDETTLPREADAATTE
                     TRMDIAPILATAKEGAEEASESPLETEATRRANIRMGNQYLDMEMLGDARIWYTAAGA
                     TEQLIHLCDRLIEREQFGEARSIFVEMGIEMPAEKFEAAGDACFARRKFSAAHIAYVC
                     AGATEKLLTLGDACFEQGQDQVGEWAYRDANTEVPAGKFEIAGDACFKAGLMEQASRN
                     YKRASATEKLIAVGDKCLETGDLTVAVEAYSAAGTKDKLVAVGDAWFEQGRPDAALKV
                     YARAGATEKRVMVGDLYLKRGMVDDARDAYKYAGEEILQKKAGALAEANVKRGDYWLR
                     EKKYDLAINHYVAAGLDVAREKLIACGDMLLEAKNFEPALRAYTAAGVALPQKTLLAI
                     ANGFDSHGQRQSALETYIAAGSVEDLVRIGRYYRASDTRD"
     gene            complement(1450..2379)
                     /locus_tag="VNN25_24920"
     CDS             complement(1450..2379)
                     /locus_tag="VNN25_24920"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXH94841.1"
                     /translation="MVSENSAAIATLDDRLRGFLRYVRTVKNQSPQSIAWYEKSYASY
                     RRYLTTLLHFPPAEFAAKSRAIDAWVQWNRERGLSAISTNNYWRGLRAFFRDIEKRGG
                     LLSPFTGISAPRFPKSEPKAKTADECRRIMETAEHYPWGSDYTRALAVATIATMLYAG
                     LRRSEIIRLRYGDVDLDRQTLLVHEGKGAYGGRDRRAYVSDELRSILARYLRERTKRG
                     YASPEFFTSSKTKQGITYESLRRLIDRVRGASGVRFTAHTLRHSFVTHLLKSGVPLHV
                     VSKLAGHRQLSTTMGYAAIFERDLADGIEQLHF"
     gene            complement(2820..3980)
                     /locus_tag="VNN25_24925"
     CDS             complement(2820..3980)
                     /locus_tag="VNN25_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94842.1"
                     /translation="MDLPLDEIADLIDRVTEDFVSHQRDPASLTTLCGIELFQMLKAD
                     LVGRRLHPTGRSKRELREATFARSSAIRPNRHVDTLSFTKTPEQVAHSLLKRKGIFDD
                     PVTGRRLVIKGPTYQARGEKGDEQMFYLVIEECSDIALAPRHALLQTASPGETTMQGD
                     TVMAPPDLDSLSIPVAMTQIGSPTIYVHIVNEDDVDVADAPIPTQSHLVSESTKTYVA
                     AKMHHLTLESFGVVFVTFEFEDVKLEGERCSVQWLDGITYRGTAYATIRLPPTMDLAG
                     LPTLLAVHAVPAGIRQLSIQYSPKQPELVWRGMLDTVRHLDPRPLWSHDSDAGEGLIW
                     GPGPGNTHFHVHWNPENFTVSFSVNDPTDFRDAMRDFPLRSFLRDKLGILAV"
     gene            4191..>4653
                     /locus_tag="VNN25_24930"
     CDS             4191..>4653
                     /locus_tag="VNN25_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94843.1"
                     /translation="MGQASSEREKTSRTLRPFDGFTRKIMRKIVKTRQTTSHLELTNE
                     SSSSVQNPHLHFRQAILIVAAVILSLFGCTAQDSAHGAKPTRSTSGPTHGTAAGDSKP
                     CLGWPKRMVFLFDQSKSTQKTRTEHPAVERLDDVIECSKRHGGSIYAGPIRD"
BASE COUNT          931 a         1391 c         1340 g          991 t
ORIGIN      
        1 gtcgcgagtg tcggaagccc ggtagtacct gccgatacga acaaggtctt ccacggatcc
       61 agcagctata tacgtctcga gcgcactctg acgttgcccg tgcgaatcga agccattagc
      121 tatcgcgaga agtgttttct gtggcaacgc gacaccggcg gcggtgtagg ctcgcagcgc
      181 tggctcgaag tttttcgcct ccagaagcat gtcaccgcaa gcgatgagct tctctcgtgc
      241 gacatcgagg cccgctgcta catagtgatt gatggctaga tcatactttt tctcacggag
      301 ccagtagtcg cctcgcttga cgttcgcctc ggcgagcgct ccagccttct tttgtagaat
      361 ctcttcgcca gcgtatttgt aagcatcgcg ggcatcatcc accatccccc tcttaagata
      421 gaggtcccca accatgaccc gcttctcggt cgctcctgct ctggcgtaga ccttaagagc
      481 agcatcaggc cgtccctgtt caaaccacgc atcgccaacg gcaacgagct tgtctttagt
      541 gcccgcagcg ctatatgcct caacggcgac ggtaagatcc cccgtctcta agcacttgtc
      601 tcccacggcg ataagcttct ccgtggcact tgctctcttg tagtttctgg atgcctgttc
      661 cattagcccc gccttgaaac acgcatcacc agcgatctcg aactttcccg caggaacctc
      721 cgtgttcgca tcacggtagg cccactcgcc aacctggtcc tgaccttgct cgaagcacgc
      781 gtcgccgagc gtcaagagtt tctcggttgc tccggcgcat acgtaggcga tatgggcggc
      841 actgaacttt cttcgtgcga agcatgcatc cccggccgcc tcgaatttct ccgctggcat
      901 ctcgatcccc atctccacga aaatcgagcg ggcctcgccg aactgttctc tctcaatcag
      961 gcggtcacag agatggatga gctgctcggt ggcgccagcg gccgtatacc agattcttgc
     1021 gtcgcccaac atctccatgt ccagatactg gttgcccatc cggatgttcg ctcggcgcgt
     1081 tgcctccgtc tccaagggcg actcgctcgc ttcttcggcc ccctcctttg ctgttgcgag
     1141 gatcggcgcg atgtccatgc gcgtctcggt cgtcgctgca tcggcctctc ggggtagcgt
     1201 cgtctcatct aaggaggctc gtggctgggt tgttatatca tcgaccggat gttgggtgtc
     1261 gtctttgccc ctggggtcgg gcgtagtggg aggcatggga agggaagata cggaatgggt
     1321 agtgagaaaa gcttcatata aatctacatg aattatcatg ttgtcgcaag ctgtagccat
     1381 tcctgaagcg gaggacgcca gtcgtgtcgg cggctgcgtt ccaccgtgaa cacagccgcc
     1441 tccgcagcgt cagaaatgca gctgctcgat cccgtccgcg aggtctcgct cgaagatcgc
     1501 tgcgtagccc atcgtggttg agagttgccg atgacccgcg agcttgctta ccacgtgcag
     1561 cggcacgccg ctcttcaaga gatgcgtgac gaaggaatgg cgcagtgtat gcgccgtgaa
     1621 gcgcacaccg gaggcacccc gcaccctgtc tatcaggcga cgcaggctct catacgtaat
     1681 cccctgcttg gtcttcgacg aggtgaaaaa ctccgggctc gcatatccgc gcttcgtccg
     1741 ttcgcgcaga taccgcgcca ggatgcttcg cagctcatcg gacacgtatg cgcggcgatc
     1801 tcgtccgcca taggccccct ttccttcgtg cacgaggagt gtttgcctat cgaggtccac
     1861 atcaccatag cgcaggcgga tgatttcgct gcgacgaagg cccgcataga gcatggtcgc
     1921 gattgtggcc acggccaggg cgcgggtgta gtcggagccc cacggatagt gctcggcagt
     1981 ctccatgatg cggcggcatt cgtcggcggt cttggccttg ggctcgctct tggggaagcg
     2041 cggcgcggag atgcccgtga acggcgagag caggccgccg cgcttttcga tgtcgcggaa
     2101 gaaggcgcgg aggccgcgcc agtaattgtt cgttgagatg gcagagagtc cccgctcgcg
     2161 gttccactgc acccaggcat caatcgcccg gcttttcgct gcaaactccg cgggcgggaa
     2221 gtgcaggagg gtcgtcagat accgacggta gctcgcatag gacttctcat accacgcaat
     2281 cgactgcgga ctttggttct tcacagtccg cacgtagcgc agaaagccgc gcagccgatc
     2341 atcgagcgtc gcgatcgccg cactgttctc ggaaaccaca ccgggaatcg gccgagcatt
     2401 cgtgctcctg tggtcgtcat tgggtgacga cgcacttggt gttatgaatt gggagggaaa
     2461 catagaagtg agaggtagct tctggcttcc tcttcccctc gatctggtcc gccgccgtgc
     2521 gccaagtgcc ggttcgaagc tcttggctct gtaccttggc gtaaggcgca caagaattcg
     2581 gccacaacac cttgcacagc gcttctatac gcgaaatctt gggcgaggtg ggattcgaac
     2641 ccacgaccaa gggcttaaga ggcagcaaaa ctgactattt gtcaattcgc cgcatcctga
     2701 cgccatttgg catggatttc tgccatgtgc ttgcgcgcat cttggcaatc agatcgaaag
     2761 gagttgagaa ggaccagtgc gatgcaggtg tagcagagac acaagccatt gtgaagagcc
     2821 tagacagcca agatgcccag cttatcgcgg aggaagctcc gcaatgggaa atcacgcatg
     2881 gcatcgcgga aatcggttgg atcattgact gagaatgaaa ccgtgaaatt ctcgggattc
     2941 cagtgtacgt gaaagtgggt gttgcccgga ccaggccccc agatcagacc ttcgccggcg
     3001 tcggagtcgt gcgaccaaag cggccgtggg tcgagatgac gaactgtgtc gagcattcca
     3061 cgccacacga gttcaggttg cttcggcgag tattggatgg agagttgcct gatgccagcc
     3121 gggactgcgt gcacggcgag aagcgtgggt agtccagcaa gatccatggt cggcggcaaa
     3181 cggatcgtcg cgtaggccgt tccacgatag gtaatgccgt ctagccactg aacactgcag
     3241 cgctcacctt ccagtttcac atcctcgaac tcgaaggtta cgaatacgac accaaatgac
     3301 tccagtgtga ggtgatgcat tttcgccgct acatatgtct tcgtggattc actcacgaga
     3361 tggctctgtg tcgggatggg agcatcggca acgtcaacat cgtcttcgtt aacgatatgt
     3421 acataaatgg tcggactccc gatctgcgtc atagctaccg ggatcgacag gctgtcgaga
     3481 tcgggcggag ccatcaccgt gtcaccttgc atcgtagtct cgcccggaga ggccgtctgc
     3541 aagagcgcat ggcggggcgc cagcgcgatg tctgagcact cctcgatcac gaggtagaac
     3601 atctgctcgt cgcccttttc cccacgtgcc tgataggtcg gccccttgat gacgagtcgg
     3661 cggccggtca ccggatcatc aaaaataccc ttccgcttca aaaggctgtg cgcaacctgc
     3721 tccggtgtct tcgtgaacga cagcgtgtct acgtgtcgat taggtcgtat cgcagaagat
     3781 cgcgcgaacg tcgcctcccg cagctcgcgt tttgagcggc cggtcgggtg cagcctacgg
     3841 ccaacgagat ctgctttgag catctggaag agttcgatcc cgcagagcgt cgtcagcgac
     3901 gccgggtcgc gctggtggga gacgaagtcc tctgtgacgc gatcgataag gtcggcgatc
     3961 tcgtccagcg gaaggtccac gattgattgt acttctgtgt agtcctttcg ccctgctgtg
     4021 cccttctcgg cctaaatctg cctaccccga cgccgccttc tgccctgtgt cactgccagg
     4081 aggaggtgac cagagtcgca tcggttgatc gcatcgatca aggggtcgga gggagcccgg
     4141 cctgctgcga gtaacacaca ccacgagaaa gacctacccc gcggtggaac gtggggcagg
     4201 cgtcgagcga acgagagaag acctcccgca ctctccgtcc tttcgacggc ttcaccagaa
     4261 aaataatgag aaaaatagtg aagacacgtc aaacgacatc ccaccttgag ctaaccaacg
     4321 aatcttcttc aagcgttcag aacccacatc tccacttccg tcaagcaatc ctgatcgtcg
     4381 cggctgtgat cctcagtctg ttcggctgca cggcccagga ttcggcccat ggtgcaaagc
     4441 ccactcgcag cacttccggg ccaacccacg gcacggccgc tggcgattcg aagccgtgcc
     4501 ttggctggcc gaagcggatg gtcttcctct tcgatcaatc aaaatcaaca cagaagaccc
     4561 gaaccgaaca tccggcggtc gagcgccttg atgatgtcat cgagtgcagc aagcgccatg
     4621 gtgggtcaat ctacgcaggt ccgatccgcg aca
//
LOCUS       DATLVW010000854         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_369871, whole genome shotgun sequence.
ACCESSION   DATLVW010000854 DATLVW010000000
VERSION     DATLVW010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369871"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VNN25_24935"
     CDS             complement(<1..263)
                     /locus_tag="VNN25_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94844.1"
                     /translation="MTSDSPIRVEVSPREAYERRPQLFAALEAAFPVKFQVGSGNADA
                     AIVFATRAVRSEPASGPTLTLLGEVQTDQPFAAIAFADSSSVP"
     gene            complement(277..1230)
                     /locus_tag="VNN25_24940"
     CDS             complement(277..1230)
                     /locus_tag="VNN25_24940"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HXH94845.1"
                     /translation="MHDAMVTGGVTSGDESQGVRARSLEKAFLKANRRVWTRLPIRIR
                     DLRFVQAYGRWLHILACRNADREMYFGTLFLRNRPALELMRRLAVKRRGSTLKMAILG
                     CSVGVEVYSILWTLRRDRPDLEIAVDAVDISPEVLAIAEQGAYGPQTSEAVHASIFQP
                     LTDAERREMFDWEGEQARVKPWLREGVAWRLGDASDPGLVDELGAADFVVANNFLCHM
                     DPSAAERCLRNIAGLVKPDSYVFVSGVDLNVRTKVALDLGWEPEVELLAEIHDGDPLV
                     RADWPFEWWGLEPLDRKRPDWQTRFASVFRVPEAPPQNQIS"
     gene            complement(1323..2591)
                     /locus_tag="VNN25_24945"
     CDS             complement(1323..2591)
                     /locus_tag="VNN25_24945"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HXH94846.1"
                     /translation="MTKHKPTKICLFGHFGSANLGNEATLLAMVSRLRAAFPDCELCC
                     ICTGPEDVVAAHAINGVPHTARSRRIWDRRLSLPKRLGMLFLGLGDEIGEYRRAWRIL
                     SGTEMFIVPGTGLLTDAFELSGWGPYGLFRWSLVARLRRCKVMFVSVGVGPVQTPKGR
                     LLVKASLAFADYRSYRDVPSKEVVEGLGLRVSGDRIYPDLVFGLPSPGSSAPVIAREG
                     RPTVALGLMAYSEKYSKGDAVGGTYERYVRSLVVFADWLLDRDYNIEFLVGDAAVDTT
                     TIADVKAALRRRTADVDERLVHRSIASPQEFLSHLGGTDFVIATRFHNVLMSLLVGRP
                     VVAISFHHKCSSLMSEMGLSDYCLDINGISPDRLIAKFEALVQHSDELESTIRQHVDE
                     SRSALDEQYELLFREELGELRFVETETAAG"
     gene            complement(2618..>3451)
                     /locus_tag="VNN25_24950"
     CDS             complement(2618..>3451)
                     /locus_tag="VNN25_24950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94847.1"
                     /translation="SYEVVVVDNGSVDGSGDALSRQTGITLIRNERNLGYAAAVNQAY
                     RHSSGEFVLLLNSDVDLTPTALSSLTDFLQDNPSAAAVGPLYLNLDGSPQPFHFRFPT
                     FKMTLANGGAVASRLLPGSKRRLRHYRMLDDDFSQPRPVPQPSASCLLLRRSCLPEDH
                     VFDERYPIFFNDVQLARSFADRGLTLWVTPAAKVVHHSHASTRKLGAAGKRQYLGSLI
                     RMLEETEPSVNVWLYRLVVFVQNIPVWLFRRPNALTFGDLWKALSGDVGPLPSDPSPP
                     A"
BASE COUNT          671 a         1095 c         1083 g          602 t
ORIGIN      
        1 ctgggtaccg agcttgagtc ggcgaacgcg attgcggcga atggctgatc ggtctggacc
       61 tcaccgagaa gcgtcagcgt cggcccactc gccggctcgg accttacggc ccgggtggcg
      121 aagacgatgg cggcgtccgc gtttccgctg ccgacttgga atttgaccgg gaaggccgct
      181 tcgagcgcag cgaacagctg cggccgtcgc tcgtacgcct cgcgcggcga cacctcgacg
      241 cgaatggggg agtcggaggt caccaggcgt agtcgattac gagatctggt tttggggcgg
      301 cgcctccggc actcggaaca ccgaggcgaa acgggtctgc cagtcgggtc gcttgcgatc
      361 gagcggctcc aagccccacc attcgaatgg ccagtcagcg cgcaccagcg ggtcaccgtc
      421 gtggatctcc gccagaagct ccacctcggg ctcccagcca agatccagcg cgaccttcgt
      481 ccggacgttc agatcgacgc cggagacgaa cacgtacgaa tccggcttca cgagccccgc
      541 aatgttccgc aggcatcgct ccgctgccga cggatccatg tggcagagga agttgttcgc
      601 aacgacgaag tcggctgcgc cgagctcgtc gacgagtccg gggtcggaag cgtctccgag
      661 tcgccaggcg acgccttccc gcaaccacgg cttgacgcgc gcctgttcac cctcccagtc
      721 gaacatctcg cgccgctccg cgtcggtcaa cggctggaag atcgaggcgt gcacggcttc
      781 cgaggtctga gggccgtacg ccccctgctc tgcgattgcg aggacttccg gtgagatgtc
      841 gaccgcgtcc acagcaatct cgaggtcggg gcggtcgcgg cgaagcgtcc agaggatcga
      901 gtagacctcc actccgacgc tgcagccgag gatcgccatc ttcagcgttg accctcgccg
      961 tttgacggcg agtctgcgca tcagctcgag cgccggacgg ttgcggagaa acaacgtgcc
     1021 gaagtacatc tcgcggtcgg cattccggca ggcaaggatg tgcagccacc gcccgtaggc
     1081 ctggacgaag cgcaggtcac ggatgcgaat cggaaggcgt gtccagaccc gccggttcgc
     1141 cttcagaaaa gccttctcga gcgaccgtgc tctcacgcct tggctctcat cccccgacgt
     1201 cactccgccc gtgaccattg cgtcgtgcat ctaggttcgt ccgcccctgg ccaactgacg
     1261 ccggtcgtag accccgtcca gcgccgccac ggcttggcgg cccgtttcgg cgctgacgtt
     1321 cctcatcccg ccgccgtctc ggtctcgacg aaccggagtt cgccgagctc ttcacggaaa
     1381 aggagctcgt attgctcgtc cagggccgat cgtgactcgt cgacgtgctg ccgaatcgtg
     1441 ctctcgagct cgtcggaatg ctgcaccagt gcctcgaact tcgcgatcag tcgatcgggg
     1501 ctgattccgt tgatatcgag gcaatagtcc gacaatccca tctcgctcat caatgacgaa
     1561 cacttgtggt gaaacgaaat ggccaccacg ggcctgccga ccagaaggct catgagcacg
     1621 ttgtgaaagc gcgtcgcgat cacgaaatcg gtcccgccga gatgcgaaag gaactcctgt
     1681 ggcgacgcga tcgagcggtg gacgaggcgc tcatcgacgt cggccgtgcg ccgacgcaac
     1741 gcggctttga catcggcgat ggtcgtggtg tctacagctg cgtctccaac caagaactcg
     1801 atgttgtagt cgcgatctag cagccagtcc gcaaacacaa cgagtgatcg aacataccgt
     1861 tcgtacgtgc cgccaacggc gtcacctttg ctgtacttct cggagtaggc catcaagcca
     1921 agagcgacag tcggtcgtcc ttcgcgcgca atcaccggcg cggacgatcc cggtgacggc
     1981 aagccaaaca cgagatccgg atatatgcgg tcgccactta cgcgaaggcc taagccctca
     2041 acaacctcct tgctcgggac gtcccgatac gaccggtagt cagcaaacgc aagcgacgct
     2101 ttcacgagta atcggccttt tggagtttgg actggcccca ccccgacgct cacgaacatc
     2161 accttgcaac gacggagccg cgcgacgagt gaccacctga acaggccata cggcccccag
     2221 cccgaaagct caaaggcgtc tgtgagcagc cccgtgccgg ggacgataaa catctccgtg
     2281 ccgctgagga tcctccaggc cctccggtac tctccgatct cgtcgcccag cccgaggaac
     2341 agcatcccga ggcgcttggg caacgaaagc cggcgatccc agattcgtcg cgatcttgct
     2401 gtgtgcggta caccgttgat cgcatgggct gcgacaacgt cttcagggcc cgtgcaaatg
     2461 cagcacagct cacaatccgg aaaggcggcg cggaggcgcg agaccatcgc taaaagcgtg
     2521 gcctcgtttc ctaagttcgc cgatccgaag tgaccaaaaa ggcagatctt cgtaggcttg
     2581 tgtttcgtca tgtcagcttc tggttccgcg ctactactca cgcaggcgga cttggatcag
     2641 acggcagcgg tccaacatca cccgacagcg ctttccacag atctccgaaa gtgagtgcgt
     2701 tcggtctacg gaataaccac accgggatgt tttgtacgaa aactacgagg cggtaaagcc
     2761 atacatttac ggatggctcg gtttcctcga gcatccgaat gagggaacca aggtactgcc
     2821 tctttccagc agcgccgagc ttgcgcgtcg acgcgtgcga gtggtgaacg acctttgcgg
     2881 caggcgtaac ccagagcgtc aggccccggt ccgcgaacga gcgcgccagc tggacgtcgt
     2941 tgaagaagat cgggtaccgc tcgtcgaaga cgtgatcttc ggggagacac gagcgccgca
     3001 gaagcaggca gctcgccgaa ggctgaggca ccgggcgcgg ctgggagaaa tcgtcgtcga
     3061 gcatccggta gtggcggagc cgccgcttgc tgcccggcag gagcctgctg gcgacggcac
     3121 caccgttggc caacgtcatc ttgaaggttg ggaacctgaa gtgaaacggc tgcggtgacc
     3181 cgtcgaggtt caggtacagg gggccgaccg cagcggcaga ggggttgtcc tggagaaagt
     3241 cggtgaggga gctgagcgca gtcggcgtga ggtcgacgtc ggaattgagc aggagaacga
     3301 actctccgct cgagtgccgg tacgcctggt tgacggcggc tgcatagccg aggtttcgct
     3361 cgttgcggat gagcgtgatc ccggtctggc ggctgagcgc atcaccggag ccgtcgaccg
     3421 agccgttgtc gacgacgacg acctcgtagg a
//
LOCUS       DATLVW010000855        21663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_488666, whole genome shotgun sequence.
ACCESSION   DATLVW010000855 DATLVW010000000
VERSION     DATLVW010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488666"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1486)
                     /locus_tag="VNN25_24955"
     CDS             complement(131..1486)
                     /locus_tag="VNN25_24955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH94848.1"
                     /translation="MLRKIFVSFWLTVVVAGLAFHGASTALHQAELRSNEREQRLVAA
                     AREAAEAWERGDKARAAVTMAAINRALGTNANVLDARGQSILGIPPGAAERGFVGTVA
                     RFFARGVHGVVGDSGVQLVGETFESTGAARYTVVTPARRNPFFWLATSRQSTDLVLGV
                     ILILSGVACFVLARHFAAPLTQLGAAANAVADGRLETRVSERIGARRDEVGTLARDFN
                     RMAERIEALVAGQRRLLGDVSHELRSPLARLTVALTLARRQPDPAEYLDRIELEAQRL
                     DLLVGQLLLLARIESGVEIARSEVDLTAIVQEVVSDGDFEARASERAVVLEEADACTI
                     TGNPDLLRSAIENIVRNAIRHTAAGTEVAVMLRTTSDSVTVTVRDHGGGLAEEILDHL
                     FEPFRSASGGAGLGLAIAERVVHMHGGSAEASNAADGGVAVRITLPLLLSSHEGESGR
                     G"
     gene            complement(1453..2163)
                     /locus_tag="VNN25_24960"
     CDS             complement(1453..2163)
                     /locus_tag="VNN25_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXH94849.1"
                     /translation="MSRILLIDDDVELCDLVTRFLTGEGFIVDAVHTGAAGAERALGG
                     AYALVLLDVMLPGTNGFEVLRQIRARSAVPVVMLTAKGEILDRVLGLEIGADDYLAKP
                     FDPQELAARIRAVLRRTSPDKAAARRPALIVGDVELEPAARLVRRGGDVVELTSVEFD
                     LLDALLAEAGQVVSRESLVRNVLGRELSPFDRSIDTHVYHLRRKLGPLPNGTERIVGV
                     RGIGYLYSCSARSSSHSG"
     gene            2291..4297
                     /locus_tag="VNN25_24965"
     CDS             2291..4297
                     /locus_tag="VNN25_24965"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH94850.1"
                     /translation="MRRLVRIACLLGLLSGLAARADTPDFKGTWEGALQHEGKSTPLA
                     IEIRQADAGGVLGRLFQSGDEMAPLSEGKVEGNRLTFRVDTLDCTATLDHDVLTVTMT
                     VSHGRTFNLTMRRKALTANPEAKSDASPAPATKETTAKAALPSATEYPPGAAVTSLAS
                     RGALILAGGGKNAPGIMQAFTRLAGRGAPIVVIPTALEEKDVKERLAVLKSRHGELFQ
                     TDDITILDTRDRAVADSEEFVKPFRRARGVWILGGEAAYLIKIYQGTRTEKELKAVLA
                     RGGVVGGTSAGAIIQTSTIIAADGRTLVPGFGLISGVLLFPHWSERRGEAGLVKYTGQ
                     IHAPLGIGVDEATAVVVQGNSFEVVGEGHVGIVDGATHEGKGYYLLNPGERFDLAGRV
                     QIHEKSADKTTEAIGKAMAAYGPDRFRGNVLVARGGRVLFRYANTTETRFRIGSLTKQ
                     FTAAATLLLEERGALRTSDPIRRYLPESPPEWSDVTIWHLLTHTSGIPSDGSARLSFK
                     PGQEWTYSNLGYQLLGTIIERVTKQTYGAFVTENILRPLGMNDSTTDPELADLGGAGS
                     LLSTTDDLLKWQQALFGGKLLKPTSLAKMTTPFRNGYGCALHLAVSASGRHVFANEGR
                     VPGSNASLQYYSEDKLVIAVLANLDTQVDALIARDLARAALEAQ"
     gene            4499..5794
                     /locus_tag="VNN25_24970"
     CDS             4499..5794
                     /locus_tag="VNN25_24970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXH94851.1"
                     /translation="MRNLSDQMQASMTKAANARAVEKTSTLGGVWRLLPALLLVVLLA
                     PPLMAATQLYPPWFPQSLFTINPDNTTVEDFGTDTFRVGKTDDPQTIEAKGHHWAGSL
                     YPPGPTSTWDHWNGVAVFKRVQAQLEASGFKLVFLNADNDGAHGTFRRGNGPEATYVD
                     LSLKNDAYSNSVAIVEPAAKARTLALRPPGAQPEAFTDAQDFPYITPLAGAKLLTTRH
                     DDGPLNVAVADHDPQYVGTGTQSKMYRGPANVSPLDFSSTYEGAFRNAGWTVTENNGA
                     TVTAHYAKNGRDLWARVFQEGADVWDVTVADVGAGLKGALTKNCRVALYGINFDFNKA
                     TIRAESEPVLQQVASVMKSLAGAFEVGGHTDNVGGAAYNAKLSQARAESVKSWLVAHG
                     IAASRLTAKGYGDTVPVVPNSSDANRARNRRVELKKAGC"
     gene            5889..6731
                     /locus_tag="VNN25_24975"
     CDS             5889..6731
                     /locus_tag="VNN25_24975"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH94852.1"
                     /translation="MSAFSSGAGEVPLTVTQPGSAVVFEWRELWASRELLLLLMWRDV
                     RVRYKQTMIGVLWAVLQPLLAMVVISAFFGRPAQLPSEGVPYPLFAYSGFLVWTFFSN
                     ALFSSSQSVVGSAPLITKVYFPRVLVPIAAIGARLVDFAIAFVLLAALMAYYGVPVTW
                     RVAALIPMIALTMALALAVGMCLAALNVTYRDVNVALPHVMQLLLFATPVFYSATILP
                     PRLKLAAQLNPLAVLIEGYRAALFDRPVHWRAIGIEGVVTAVLIVCAVAVFRRMEEHF
                     ADVL"
     gene            6734..8017
                     /locus_tag="VNN25_24980"
     CDS             6734..8017
                     /locus_tag="VNN25_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH94853.1"
                     /translation="MPIAIKAEGLGKRYTIGRSRETGLRDALAHAARAPLRLFRTPGA
                     AGGGNEMWALRDLDFEIERGETVGIIGRNGAGKSTLLKILSRVTRPTTGRAMWRGRLT
                     SLLEVGTGFHPDLSGRENIFLNGAILGMSQAGVRRKLDEIVAFAEIETFLDTPVRHYS
                     SGMYMRLAFAVAAHLEPEILVLDEVLAVGDAAFQQKCQSFIERIVGSGATLLLVSHNL
                     HAVSTLCSRVLFLDGGSLRRDGRTEEVIADYIESVSPSGDAAEIEWGNRDDAPGNKNI
                     RLHAIRVVSGESTRFSVDIRVPLRVEIDYWNFVPGTRIYTSIHLVEKTGVTVLASANM
                     PSFNTAEDDWYGRPQPAGLYRSVCTFPADLLNEARYSVSAFIVADMARLEAAAHHAVS
                     FHAVDINREREHHAIVPGVIRPKLAWRTELVQPLP"
     gene            8090..8812
                     /locus_tag="VNN25_24985"
     CDS             8090..8812
                     /locus_tag="VNN25_24985"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXH94854.1"
                     /translation="MNLASLRDRFRAKTEIFQTPECRMHVHLDERERHIDYAICHEVQ
                     IDDCYGVRKLAESAFTPRVIVDIGAHIGTFTALAAKYFPQARIYSFEPLKQHYQVLVK
                     NSRSGHCKPNHLAVLGFYGSEEGRDIYPSNEFERAYRARQLSNAVSVAQMLRKFKLQH
                     IDWLKIDCEQSEVNIFRELDFLDRLKDIDLISGEWHFETAKSELRTIVGKTHDVEIVD
                     EGHWNYFEARRKPTVARGGDGA"
     gene            8809..9732
                     /locus_tag="VNN25_24990"
     CDS             8809..9732
                     /locus_tag="VNN25_24990"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH94855.1"
                     /translation="MKLCKIYGLADRLDDDPLWHELLDAMTPEWRTLPALHRKYWEWG
                     LGLYGLHKLGCIRAGAVGLGVGAGVEWPLYYLANHVGRVEAIDLYARESAFMNLDPTF
                     PENARRLAPFPYREEALVFSKMDALDLKFPDKTFDFVFSFSSIEHFGGHPGAVLAMQE
                     IARVLKPNGVAAIATEVVLNGQWHSEFFQPEELEPTFVGGSGMQLVEPLDLSVDEEMI
                     TNPVLFDVPPGFTGDTGPHTSVKAGNFIFTSIELFLRKPENWKPMSKRAMAAVRKDRA
                     AWYGRQPRPVPLPQFVKRPLKKVVQLFGWRR"
     gene            9729..10877
                     /locus_tag="VNN25_24995"
     CDS             9729..10877
                     /locus_tag="VNN25_24995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH94856.1"
                     /translation="MKIAMVTTVGQRCGIASYSASLAESLRLVPETEIEIVPITVGAQ
                     PSAHYEEQARALNAASVDVVHFQHEFSFWGFPPPNGRSAFAELRALVRKPTVVTAHTT
                     LGLDAIFPMTSRRNPVRWLRRKRLLAHHGWRESVEVKTFDADATIVHTETAHNEFAQR
                     GLDRQRLFVVPMGIPSPLPAATAGQAFRERWGLHGKRLLTLFGYITPNKGYTLVADAL
                     RHLPDDVVLVIAGGARRPIEQQYVDAVERHVRKIGMTRRVIITGYLSDAEVAEAMAAT
                     DLALAPHTQATNSYSVAFPLTYGKPTLASDLPCFREMAATSDSLELFENRSKADFRRK
                     LVALLEDGARREQLAANALRHAERFRWPNVAQATRDVYAKAAARHRRS"
     gene            10891..12240
                     /locus_tag="VNN25_25000"
     CDS             10891..12240
                     /locus_tag="VNN25_25000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH94857.1"
                     /translation="MTQTNRALVISTVGNDEPAADRMGSASYSYHYVCQSFLPLLRRW
                     GGVTVAARDLETADGAIHLSFRPLDFFCPTAGARNAAFPFWDFPDVPAYALGGNPRND
                     WVAIANSLDLLLTSSEFTRDAFMRSGVRVPIRVVPVPVRRQFFELAAWEPQRRLLDCP
                     AYELTSDGVPRLPASSHATSVYHRRVRPHLSRRSIDLASALGRSARTARAEWRDITSA
                     APPPLSCVELSGIVYTTVLNPFDQRKNWEDLLTAFLFALRDHSDATLVVKLVVPPQRA
                     AFGVNRIVRFWRTLGIGHRCRVVLISAYLDDAQMLELTGASTYYLNASRAEGACLPLQ
                     DFLAAGRPAVAPRHSAIADYFDSGSGLVVESHPEPTSWPQDPDGRCTTIWHRLVWTSL
                     RDRIAESYDMATHDRAGYARLSANAQARMHARASDEAVWAQLEPALDSIEQRDRRAL"
     gene            12230..13660
                     /locus_tag="VNN25_25005"
     CDS             12230..13660
                     /locus_tag="VNN25_25005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXH94858.1"
                     /translation="MLFNSFVFIGFFVVVYAAYVVLRHQAQNVMLLLASWFFYGSWDW
                     RFLGLMLFSTAMDYAFGRLLARVQDPRRRKLILVTSVTINLGILFVFKYFNFFAATLG
                     GMLHVFALDPGAPLLQVVLPLGISFYTFHNISYIVDVYHERVEPTRDFVEFGLYVALF
                     PQLIAGPIARPAHLLPQIRTPRRITYEMFRTGAWLILLGYFKKVVLADNMAAFTKPLF
                     ANPQAFHGLEVLFGLYAFAFQIYGDFSGYSDIARGLSKLMGFELMVNFRHPYFATNPA
                     DFWRRWHISLSTWLRDYLYIPLGGNREGTLKTYRNLMITMLLGGLWHGAAWHFVAWGA
                     FHGVLLCVHRAAEPLLASLRSMAAASSVFVRRVGRAGAVFAFFNVTCIGWLLFAAPSL
                     RDAGTLLRNTVAPALTHGRLVAVTMLCFALPLLAMEWAQERSGAAVPVVKTWPRALRL
                     ACYIAVFSAILLCGAVERHEFIYFQF"
     gene            13635..14690
                     /locus_tag="VNN25_25010"
     CDS             13635..14690
                     /locus_tag="VNN25_25010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94859.1"
                     /translation="MSSSIFSSETVRRYGALPRKERFWHAAIAVAAAIAVLVCREIPP
                     RLYGTSGCQGGSLGSTAVASLSDRTEVLFAGSSHVLFGVRPQRFTVHAMNLAGTWLDY
                     TCIRAVVARHLHRVPNLKVAVIEYDELPYVSDLVPAIVATRDVRPLQELGLSPAEFPT
                     AGAMQAGEVFATSFMFPFTGLPRVTPLAWRNRDKSCSPLYHPPRGFAPGYYYTDGVTP
                     KNFNAGAVFNSLAKASQKVNVVRRNAAALTATIALLRSRGTSVVLLRLPHARDYVTQR
                     PPIVSARFRELQRIAIAASRSDAGIAVWDLGEDPAFAPADFCDNNHLNAAGADKLAKL
                     LDAPLRALASKTVAEVP"
     gene            14687..15910
                     /locus_tag="VNN25_25015"
     CDS             14687..15910
                     /locus_tag="VNN25_25015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH94860.1"
                     /translation="MKIVLCKGRFAGPISGADETIVAYAIELRAAGHDACVTVLHPPR
                     ADDPHYRRLLDARVPVSCIAGRSLVGRGMQFVRNHMPHIPTGPRRLLQKTAAGLSSSY
                     DRRCHAYFARLQPDVVHVVTPDPASVAIIRGAHAANVPVLYQELGTADFLPDLNFYYD
                     HLAAALPLAHVAALSPSLAARFGEKFGRTAVDVLPITLADVAAPAPRSSDGRVTFGFA
                     ARLEYGKGPLALVDAFVKTADRVPDASLRIAGGGPQFDDVMSRAAALALNGRCTFTGT
                     YAGPAARDAFMRSIDVLVLPTLAEGTPNTIVEAMAHAVPVIASAVGGIPDMLGSDAGV
                     LVRPADIDALAAAMTELGRDAARREMLARAARARYEELFSPVAVVPLLLATYARLAGH
                     DDADPAPRHPWWNGR"
     gene            complement(16004..16080)
                     /locus_tag="VNN25_25020"
     tRNA            complement(16004..16080)
                     /locus_tag="VNN25_25020"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16044..16046),aa:Met,seq:cat)
     gene            16430..17629
                     /locus_tag="VNN25_25025"
     CDS             16430..17629
                     /locus_tag="VNN25_25025"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXH94861.1"
                     /translation="MRRFLLILLLASAARATTLPGFHVQVLQGTPGFLTALVVDSQGT
                     IYYSTKSGDVLRVGPAERASGKPVVVAHVTTEANGNCGLIGLALRDDNTAIVHYTTPA
                     ITYDIVSAIDLTTGAESQIHRFVCDKDVAERGVSTEHHGGNLTVAPDGSIFLAIGDYG
                     VGSLAQDPAWNAGKLWRILPYGSAVQFARGFRNPFDLAWDAATQRVIITDNGDAVDDE
                     IDVVVEGDDCGWPLTAGNRPPVAGMRPPVYTFPVVVAPTGLAALSGRNPILRRGYLLG
                     SFVAKALFYVPDIYANPLPDPVAVIQNESDPIIDVAEGPDGSIYFASGFVIYKLVVPA
                     SGDCNGDGVVDAADISALSKELSGGPHATTAAHASWGCDVNGDGVIDARDLAALAGMV
                     RVRAVRR"
     gene            17751..17906
                     /locus_tag="VNN25_25030"
     CDS             17751..17906
                     /locus_tag="VNN25_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94862.1"
                     /translation="MKRLLIASLLLIASAAQAKEVLPWIENDYKMAIARAKATDRPVF
                     VEAWAPW"
     gene            17922..19055
                     /locus_tag="VNN25_25035"
     CDS             17922..19055
                     /locus_tag="VNN25_25035"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HXH94863.1"
                     /translation="MRAYVFTDKSLERYAGRFVWLSIDTENATNAAFLKAHPISVWPT
                     LLILDPKDKVVFRYAGGGTVPQVRKLLDDGERAAQGSHGKADTLLIRADALAAQGKQA
                     EAAKVLDEAIAAAPKNWPRFGRASESLVFTLLQSQNSQACAERALALYPRVRGTGSAV
                     NVAGTGLSCASDLDPKKSWNRSELIAGLEKATRETLAAADKIHMSADDRSGLYESLAD
                     ARDEAKDAAAAHALREQWAAFLEGEAAKAKTPDQRAVYDSHRLTAYLGLKQPEKAIAM
                     LEQSERDLPNDYNPPARLISAYKAAGRFDDALAASDRALKLAYGPRKIGIYRNRASVY
                     AAKGDKESAKKTIEEAIRFAESLPTEQQSANMIASMKKQLTTM"
     gene            complement(19274..19954)
                     /locus_tag="VNN25_25040"
     CDS             complement(19274..19954)
                     /locus_tag="VNN25_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94864.1"
                     /translation="MPIVVRLLLAVTIAAATSTGVLIADTTCTQVSIITHPSFVTILP
                     GTSTTLSVTAAGTLPITYQWYTGTSGNTSQPIPNATTSSVTVTPVTTITAYWVHVANS
                     CNTVGVNSVTGVVTLSATCPSPTIDKQPLSLSAGVSTVATLKIEARHTATGTGIPGSP
                     LHYQWYKGVKGDISTKVGTDSDTFITNPVSVTASYWVRVSEECISSIDSNAAIVTPTF
                     PPRSRAVR"
     gene            complement(20094..>21663)
                     /locus_tag="VNN25_25045"
     CDS             complement(20094..>21663)
                     /locus_tag="VNN25_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94865.1"
                     /translation="TGTNTAVQWYIGNPPSTTTTASNGTTSSLTVSPTVTTTYWVRVT
                     ACGGSANSNAATVTVTTCTPPSAPAPVANPSTITSGQSSTITETPTGSGPFTYQWYSG
                     NSGVTTSPIGGSTSNNSITVSPTVTTSYWVRVTGQCTPTVDSPAVTVTVAPCVPPTAA
                     PPSASPSSITNGQSSLLSESASGTGPLTYQWFIGNFGVTTSPVGGPSSNSFITVSPTT
                     TTTYWVRVTGQCGSPSDSRETTVTVTCAPIASGPIASPQTINNGQSSILSINTAGNGP
                     FTIQWYQGSSGDTSNMINGATGTSTVVSPTVNTSYWVRVSSSCGTQDSNNVVVVVNGS
                     PCVAASIATQPASTTISSGSPVTLTVVAAGTAPFTYQWFTGDKGDTSNAIQGATLASL
                     IRSPTVTTKFWVRVTGCNNSTADSNTATITVSTCTSPSITTQPANVSAPIGTAATFKV
                     VAAGTALHYQWYEGNKGNTTKTVGTDSSTFVTDAVDFNNQYWVRITGQCGTADSNAAT
                     VTAIFPPRGRAVRH"
BASE COUNT         3736 a         7096 c         6991 g         3840 t
ORIGIN      
        1 cgaacgggtt gacgctttcg ttgacttctt cctgaatttc cgggagccga gattgtagcc
       61 cggcctttta ccgccgtcac ttgccgtcgt cagcagtccg ggtcaaggag ctcgacgcga
      121 tcaacgggcg tcaaccacga ccgctttccc cttcatggga agacagcaac agcggaaggg
      181 tgatgcgaac cgctactcca ccgtccgcgg cattcgacgc ctcggcacta ccgccgtgca
      241 tgtgcacgac acgctcagca atcgccagcc cgaggcccgc accgcccgac gcgcttcgga
      301 aaggctcgaa gagatgatcg aggatctcct cagcgagacc gccgccgtga tcgcgcacgg
      361 ttactgtcac gctatccgag gtagtgcgaa gcatcacggc cacctccgtg ccggccgccg
      421 tgtgacggat cgcattgcgc acgatgttct cgatcgcgct tcgcaagagg tccggattac
      481 cggtgatcgt gcaggcatcg gcttcctcca gaacgacagc gcgttcgcta gcgcgtgctt
      541 cgaaatcgcc gtcgctgacg acttcctgca cgatggcggt gaggtcgacc tcgctccgcg
      601 cgatctccac gccgctctcg atacgggcca gcaagaggag ctggcctacg agcaagtcga
      661 gccgctgcgc ctcgagctcg atccggtcga gatactccgc gggatcgggc tggcggcgcg
      721 ccagcgtaag ggcaaccgta agacgcgcca gaggggagcg gagctcgtgt gagacgtcgc
      781 cgaggagacg gcgttggccg gcgacaagtg cttcgatccg ttccgccatc cggttgaagt
      841 cgcgggccag tgttcccacc tcgtcgcgcc gtgcgccgat gcgctccgaa acgcgagtct
      901 ccaatctgcc gtcggcaacg gcgttcgccg cggcaccgag ctgcgtcaat ggcgccgcga
      961 aatgccgggc caggacgaag caggccacac cgctgagaat caggatgact cccagtacga
     1021 gatccgtcga ctgccggctc gtggccaacc agaagaatgg gttccggcgc gccggagtga
     1081 cgacggtata gcgggcggcg cccgtcgact cgaaggtttc gccgacaagc tggacgccgg
     1141 aatcgccgac gacgccatga acccctcggg cgaagaatcg cgcgacggtt cccacgaagc
     1201 cgcgctcggc ggcaccaggt ggaatcccga ggatcgactg gccacgcgca tcgagcacgt
     1261 tggcgttggt gcccagagct cgattgatcg cggccatggt tacagccgca cgagccttgt
     1321 cgcctcgttc ccacgcctcc gccgcctccc gcgccgcggc gaccagccgc tgctcccgct
     1381 cattcgaacg cagctcggcc tgatgaagcg ccgtcgacgc gccatgaaag gcgaggccgg
     1441 ccacgacgac cgtcagccag aatgagacga agatcttgcg gagcatgagt agagatatcc
     1501 gatgccgcgg acgccgacga tgcgctcggt cccgtttggg agcggcccga gcttgcgtcg
     1561 gaggtgatag acgtgtgtgt cgatgctgcg gtcgaagggt gagagctccc ggccaaggac
     1621 gttgcggacg agcgattccc ggctgacgac ctgccccgcc tcggcgagca atgcgtcgag
     1681 caggtcgaat tcgacgctcg tcagctcgac gacatctccc ccgcgccgga cgagccgcgc
     1741 cgccggttcg agctcgacgt ctccgacaat cagagccggg cgccgtgcgg ccgctttgtc
     1801 aggagacgtg cggcgcagca cggcgcggat ccgtgccgcc agttcctgcg gatcgaacgg
     1861 cttcgccagg tagtcgtccg cgccgatctc caggccgagg acgcggtcga ggatctcgcc
     1921 cttcgcggtc agcatcacca ccggcacggc cgagcgcgcc cggatctggc gcagcacttc
     1981 gaacccgttc gtgccgggaa gcatcacatc gagcaggacc agggcgtacg cgccgccgag
     2041 cgcacgttcc gcgccagcag cgccagtgtg gaccgcgtcg acgatgaacc cttcaccggt
     2101 gaggaagcgc gtgacgaggt cgcacagctc gacatcgtcg tcgatgagca gtatccggga
     2161 caccgctcga ttgtagacac cgagtgacgc gcgcagtgtc aagtttcgtc gagcgcgcgt
     2221 gacgcttcct gacaaacctc gaccgcacgt cgagaggcat cagtgcattc tgcggcgcag
     2281 gaggttctgc atgaggagat tggtgcgcat tgcatgcttg ttgggtttgc tgtccggttt
     2341 ggcggcccgg gcggataccc ccgatttcaa ggggacgtgg gagggagcac tccagcacga
     2401 ggggaagtcg accccgctgg ccatcgagat ccggcaagcc gatgctggag gagtgctggg
     2461 acgcttgttt cagtctggtg atgaaatggc gccgctgtcg gagggtaagg tcgaaggcaa
     2521 tcgcctgacc tttcgggtag acacgctcga ctgcacggca acgctcgacc acgacgtatt
     2581 aaccgtgact atgaccgtct cgcatggccg gacgttcaac ctgaccatgc gccgcaaggc
     2641 gctgacggcg aatcctgagg cgaagagtga tgcgagcccg gcgccggcga cgaaagaaac
     2701 aacggcaaag gcggccttac cctcggccac ggagtacccg cccggggcgg ccgtcacaag
     2761 cctcgcttcg cgtggtgcgc tcattctcgc cggcggcggc aagaacgctc ccggcatcat
     2821 gcaggcattc accaggctcg ccggccgcgg cgcgccgatc gtcgtcatcc cgaccgcgct
     2881 cgaggagaaa gacgtgaagg agcgtctggc tgtattgaag agccgccacg gtgagctctt
     2941 tcagacggac gacatcacca tcctcgacac ccgcgaccgc gcggtggcgg attcggaaga
     3001 gttcgtgaag cctttccgtc gcgcccgcgg cgtctggatc ctcggcggcg aggctgcata
     3061 cctcatcaag atctaccagg gcacccgcac cgagaaggag ctgaaggccg tcctcgctcg
     3121 cggcggtgtc gtcgggggta catccgcggg agcgatcatc cagacctcga ccatcatcgc
     3181 ggccgacggt cgtacgctcg tgccgggatt cggtctgatc tccggcgtcc tgctctttcc
     3241 gcactggagc gagcggcgcg gagaagcagg actggtgaag tacacgggcc agatccacgc
     3301 cccactcggc atcggcgtcg acgaagcgac ggccgtcgtc gtgcagggaa actcgttcga
     3361 agtcgttgga gagggccacg tcggcatcgt cgacggcgcc acacatgaag gcaagggcta
     3421 ctaccttctc aatcccggcg agcggttcga cctcgctggg cgcgtgcaga tccacgagaa
     3481 gtccgcagac aagactacgg aagcgatcgg gaaggccatg gcggcatacg ggcccgatcg
     3541 ctttcgcggc aacgttctcg tggcgcgcgg cggccgcgtt ctcttccgct acgccaacac
     3601 aacggaaacg cggttccgca tcggttcgct gacgaaacag ttcacggctg cggccacgct
     3661 gctcctcgaa gagcgcggcg cactgcgcac ctccgatccg atccgccgct acttgccgga
     3721 gtcgcctccg gagtggtccg acgtgacaat ctggcacctg ctgacgcaca cctccggcat
     3781 tcccagcgac ggcagcgcgc gcctcagttt caagcccggc caggagtgga cgtacagcaa
     3841 tctcggctac cagctcctcg gtacgatcat cgagcgcgtg acgaagcaaa cctacggcgc
     3901 attcgtaacg gagaacatcc tccgccccct cgggatgaac gactcgacta ccgatccgga
     3961 gttggcagac ctcggcggtg ccggctctct cctctccacg acggatgatt tgctgaagtg
     4021 gcagcaggcg ctcttcggcg gcaaactctt gaagcccact tccctggcaa agatgaccac
     4081 ccccttccgc aacggctacg gctgcgcgct gcatctcgcc gtctctgcaa gcggccggca
     4141 tgtcttcgcc aacgaaggcc gcgtccccgg ctcgaacgcc tccctccagt actactccga
     4201 ggacaagctc gtgatcgcgg tcctcgccaa cctcgacacc caggtcgacg cattgatcgc
     4261 tcgtgacctg gcgcgcgcag cgctggaagc gcagtagaag ccggcctcag cccgcaaccg
     4321 cctgcagcaa ggaagtcgaa gatgcggtcc ggttcgaaac ggtcctcgcc gcttgcgaag
     4381 ccattgagga tggtttcttt cgtcgatccg cgacgactcc aaacaggggt cgcgagccca
     4441 aaaaaaggca tcgatgggaa tgagtcggcg tgagtagaat tgcgtcggag gcaacgcgat
     4501 gcggaatctg agcgaccaga tgcaggcgag catgacgaaa gcggcgaacg cgcgtgcggt
     4561 cgaaaaaacg tcgacccttg gcggtgtttg gcgtctcctg ccggcgctgc tgctggtcgt
     4621 tcttctagcg cccccgttga tggcggcgac gcaactgtat ccgccgtggt ttcctcagag
     4681 tctcttcacc atcaacccgg acaacacgac ggtcgaggac ttcggcaccg acacgttccg
     4741 ggtcggcaag acggatgacc cgcagacgat cgaagcgaaa ggacaccact gggccggctc
     4801 actgtatccg ccgggcccga catccacctg ggatcattgg aacggcgtgg ccgtattcaa
     4861 gcgcgtgcag gcgcagctcg aagcgagcgg gttcaagctc gtgtttctca atgccgacaa
     4921 cgatggagcg cacggtacgt tccggcgagg caacggaccc gaggcgacgt atgtcgacct
     4981 ctcgctgaag aacgacgctt acagcaacag cgtcgcgatc gtcgagccgg cagcgaaagc
     5041 ccgtacgctc gcgctgcggc cgcccggcgc acagccagag gctttcactg atgcgcagga
     5101 cttcccatac atcacgccgc tcgccggcgc gaagctcctg acgacgcgtc acgacgacgg
     5161 ccccctcaac gttgccgtcg cagaccacga tccgcaatac gtcggcaccg gcacgcagtc
     5221 gaaaatgtac cgcgggccgg cgaacgtgtc gccgctcgat ttcagcagca cgtatgaggg
     5281 ggccttccgc aatgccgggt ggacggtcac ggagaacaac ggcgcaaccg tcacggcgca
     5341 ctacgcgaag aacggccgcg atctctgggc gcgcgtgttt caggaaggcg ccgatgtctg
     5401 ggacgtaacg gtcgccgacg ttggcgctgg cctcaaaggc gcgctgacga agaactgtcg
     5461 tgtcgctctc tacggcatca acttcgattt caacaaagcg acgattcgtg cggaatcgga
     5521 gccggtcctg cagcaagttg catcggtcat gaaatcgctt gcaggcgcgt tcgaggtcgg
     5581 cggccacacg gataacgtcg gcggcgctgc gtacaacgcg aagctctcac aggcacgcgc
     5641 ggagtcagtg aagtcgtggc tcgttgcaca cggcatcgcc gcgagccggc tgacggcgaa
     5701 aggctacggc gacaccgtgc cggtcgtccc gaacagctcc gacgccaatc gtgcgcgcaa
     5761 tcgacgcgtg gagctgaaaa aagctggatg ctgacggcgc cgcgcgcgcc cgctgcggaa
     5821 cggagtagct ccgcctgttg cgggacgacc agaacgtccg ccctggcaac cggttatagt
     5881 cctcggccgt gtccgcgttc tcatccggcg ccggtgaagt gccgctcaca gtcacccagc
     5941 ccggctcggc ggtggtgttc gagtggcgcg agctgtgggc gtcgcgcgag ctgctgctgc
     6001 tgctcatgtg gcgggacgtg cgggttcgtt acaagcagac gatgatcggg gtgctgtggg
     6061 cggtgctgca gccgctgctg gcgatggtgg taatcagcgc gtttttcggc cggcccgcgc
     6121 aactgccgtc ggaaggcgtt ccgtatccgc tgttcgcgta ctccggcttt ctcgtctgga
     6181 cgtttttctc gaacgcgctc ttcagcagca gccaaagcgt ggtcggaagc gctccgctga
     6241 tcaccaaggt ctactttccg cgcgtcctcg tgccgatcgc cgcgatcggc gcgcgcctcg
     6301 tcgactttgc gatcgcattc gtgctgctcg ccgcgctgat ggcgtactac ggcgtgccgg
     6361 tcacgtggcg agtcgcggcg ctcattccaa tgatcgctct gaccatggcc ctcgccctgg
     6421 ccgtcggcat gtgcctggcg gcgctcaacg taacgtatcg cgacgtcaac gtggcgttgc
     6481 cgcacgtgat gcaactgttg ctgttcgcaa cgcccgtttt ctactcggcg acgatcctgc
     6541 cgccgcgcct gaagctcgcc gcgcaactca acccgctggc cgtgctgatc gagggctacc
     6601 gcgccgcgct gttcgatcgg ccggtgcact ggcgggccat cggcatcgag ggagtggtga
     6661 cggcggtact gatcgtctgc gcggttgccg tattccgccg gatggaagag cacttcgcgg
     6721 acgtgctttg acgatgccga tcgcgatcaa agccgagggg ctcggaaagc ggtacacgat
     6781 cggccgttcg cgcgagaccg gcctgcgcga cgcgctcgca cacgccgcgc gcgcaccgct
     6841 gcggctgttc cggacgccgg gggcggccgg cggcgggaac gagatgtggg cgctacgcga
     6901 tctcgacttc gagatcgagc gcggcgaaac cgtcggcatc atcggccgca acggcgccgg
     6961 caagagcacg ctcctgaaga tcctctcgcg cgtcacgcgc cccactaccg gccgagccat
     7021 gtggcgcggc agactcacgt cgctactgga agtcggcact ggcttccatc ccgacctcag
     7081 cggacgcgag aacatcttcc tcaacggcgc gatcctcggc atgagccagg cgggcgtgcg
     7141 gcggaagctc gacgagatcg tcgccttcgc ggagatcgag accttcctcg acacgccggt
     7201 gcgccactac tcgagcggca tgtacatgcg gctggcgttc gcggtggccg cgcatctcga
     7261 gccggagatc ctcgtgctgg acgaagtgct cgccgtcggc gacgcggcgt ttcagcagaa
     7321 gtgccagtcg ttcatcgagc ggatcgtcgg aagcggcgcc acgcttctcc tcgtcagcca
     7381 caacctccat gccgtctcca cgctttgctc gcgcgtgctg ttcctcgatg gcggatcgct
     7441 ccgtcgcgac gggcggacgg aggaggtgat cgccgactac atcgagtcgg tctcgccgag
     7501 cggtgacgcg gcggagatcg aatggggaaa tcgcgacgac gctcccggca acaagaacat
     7561 ccggctgcac gccatccgcg tcgtctcggg cgaatcgacg cgcttctcgg tcgacatccg
     7621 cgtgccgctg cgggtcgaga tcgactactg gaacttcgtt ccggggacgc ggatctacac
     7681 gtcgattcac ctcgtcgaga aaaccggcgt gaccgtcctg gcctcggcga acatgccgtc
     7741 gttcaacacc gccgaggacg actggtacgg cagaccccag ccggcgggac tctatcgaag
     7801 cgtctgcacg tttccagcgg acctgctgaa cgaagcgcga tactccgtca gcgctttcat
     7861 cgtcgccgac atggcacggc tggaagccgc cgcgcatcac gccgtcagct tccacgccgt
     7921 cgacatcaat cgggagcgcg agcatcacgc gatcgttccg ggcgtcatcc gtccgaagct
     7981 cgcctggcgg accgagctcg tgcagccgct gccgtgacgc gagcgcacaa cgtgcgtgct
     8041 acactcgccg ccgtccgcag caaccatccg gcctgcccca tcgaacgtca tgaacctcgc
     8101 ctctctccgc gaccgtttta gggccaaaac tgagatcttc cagacccccg aatgccggat
     8161 gcacgtgcac ctcgacgagc gcgagcgcca catcgactac gccatctgcc acgaggtgca
     8221 gatcgacgac tgctacggcg ttcggaagct ggcggagtcc gcgttcacgc cgcgcgtcat
     8281 cgtcgacatc ggagcgcaca tcggcacctt caccgcgctc gcggcgaagt actttccgca
     8341 ggccagaatc tacagcttcg agccgctgaa gcagcactac caagtgctgg tgaagaactc
     8401 ccggagcggc cactgcaagc cgaaccatct ggccgtcctc ggcttctacg gcagcgagga
     8461 ggggcgtgac atctatccgt cgaacgagtt cgagcgcgcc tatcgcgcac ggcagctctc
     8521 caacgccgtc tctgtggcgc agatgctgcg caagttcaaa ctgcagcaca tcgattggct
     8581 gaagatcgat tgcgaacaga gcgaagtgaa catctttcgc gagctcgatt tcctcgatcg
     8641 actgaaagac atcgacctca tcagcggcga atggcatttc gaaacggcca agagcgagct
     8701 gcgcacgatc gtcggcaaga cgcatgacgt cgagatcgtc gacgaggggc actggaacta
     8761 tttcgaggcc aggcgcaaac ccacggtcgc ccgtggagga gacggcgctt gaaactctgc
     8821 aagatctacg gcctcgccga cagactcgac gacgatccgc tgtggcatga gctcctcgac
     8881 gcgatgacgc cggagtggag gacgctcccc gcgcttcacc gcaagtactg ggagtggggc
     8941 ctcggcctct acggcctgca caagctcggc tgcatccggg ccggcgcggt cggcctcggc
     9001 gtcggcgcgg gcgtcgagtg gcccttgtac tacctggcca atcacgtggg ccgggtcgaa
     9061 gcgatcgatc tctacgcgcg cgagtcggcg ttcatgaacc tcgacccgac gttccccgag
     9121 aacgcacgcc gtctcgctcc gttcccgtac cgcgaggagg cgctcgtctt cagcaagatg
     9181 gatgcgctcg acctgaagtt tcccgacaaa acgttcgact tcgtcttcag cttctcgtcg
     9241 atcgagcact tcggcggaca tcctggcgca gtgctggcga tgcaggagat cgcgcgtgtc
     9301 ctgaagccga acggcgtcgc cgccatcgcc accgaggtgg tattgaacgg ccagtggcac
     9361 tcggagttct tccagcccga agagctcgag ccgacgttcg tcggtggcag tggcatgcag
     9421 ctcgtcgagc ctctcgacct ttccgtcgac gaggagatga tcacgaaccc agtgctcttc
     9481 gacgttccgc cgggcttcac cggtgacaca gggccgcaca cgtcagtgaa ggcgggcaat
     9541 ttcatcttca cgtcgatcga gctcttcctg cgcaaaccgg agaactggaa gccgatgtcg
     9601 aagcgcgcga tggcggccgt ccgaaaggat cgcgcggcgt ggtacgggcg ccagccgcgc
     9661 cccgttccgc ttcctcagtt cgtgaaacgg ccgctgaaaa aagtcgtgca gctcttcggc
     9721 tggcggcgat gaagatcgcc atggtcacga cggtcgggca gcggtgcgga atcgcttcgt
     9781 actccgcgtc gctggcggaa tcgctgcgcc tcgttccaga aacggagatc gagatcgtgc
     9841 cgatcaccgt cggcgcacag ccatcggcgc actacgagga gcaggcccgc gccctaaacg
     9901 cggcaagcgt cgacgtcgtc cacttccagc acgagttcag tttctgggga tttccgccgc
     9961 cgaacggccg ctccgcgttc gcggagctgc gcgcgctggt ccggaagccg accgtcgtca
    10021 cggcgcacac gaccctcggg ttggacgcga ttttcccgat gacgtcgcgc cgcaacccag
    10081 tccgctggct gcggcggaag cgcctgctcg cgcaccacgg ctggcgcgaa agcgtcgagg
    10141 tgaagacgtt cgacgccgac gcgacgatcg tccacaccga gacagcgcac aacgagttcg
    10201 cgcagcgcgg cctcgaccgc cagcggctct tcgtcgtgcc gatgggcatt ccgtcgccgc
    10261 tgcccgcagc gaccgccggc caggcctttc gcgagcgctg gggcctccac ggaaagcgtc
    10321 tcctcacact cttcgggtac atcaccccga acaaaggcta cacgctcgtg gccgacgcgc
    10381 tgcggcacct gcctgacgac gttgtgctcg tgatcgccgg cggcgcacgg cggccgatcg
    10441 agcagcagta cgtcgatgcc gtcgaacggc acgtcaggaa aatcggtatg acgcggcgcg
    10501 tgatcatcac cggctatctg tccgacgccg aggtcgccga ggcgatggcc gcgaccgacc
    10561 tggcgctcgc accgcacacg caggcgacga attcgtactc cgtggcgttt ccgctgacct
    10621 acgggaagcc gacgctcgcc tccgaccttc cctgctttcg cgagatggcc gccacgagcg
    10681 actcgctcga gctgttcgag aaccggagca aggccgattt ccgtcggaag ctcgtagcgc
    10741 tgctcgaaga cggggcgcgc cgcgagcagc tcgccgcgaa tgcgctgcgt catgcggagc
    10801 gattccgttg gccgaacgtg gcgcaggcca cgcgcgacgt ctacgccaag gcggctgcgc
    10861 gccatcgccg ctcgtgaagg aacaccgtcc gtgacgcaga cgaaccgcgc gctcgtgatc
    10921 agcactgtag ggaatgacga gccggcggct gaccggatgg gctccgcgtc gtactcgtac
    10981 cactacgtct gccagtcgtt cctgccgctg ctgcggcggt ggggaggcgt gaccgtcgcc
    11041 gcacgcgatc tcgagaccgc ggacggagcg atccacctca gctttcgacc gctcgacttt
    11101 ttttgtccca cggccggcgc gcggaacgcc gcctttccgt tctgggactt tccggacgtg
    11161 ccggcttacg cgctcggcgg caacccgcga aacgactggg tcgcgatcgc gaactcactc
    11221 gacctgctgc tgacgtcgag cgagttcacg cgcgacgcct tcatgcgctc cggcgtccgc
    11281 gtgccgatcc gcgtcgtgcc ggtgccggtg cgccgtcagt tcttcgagct cgcggcgtgg
    11341 gagccgcaac gcagactcct cgattgcccg gcgtacgagc tgaccagcga cggtgtgccg
    11401 cgattgccgg cgtcgtcgca cgccacgagc gtttaccacc gccgcgtgcg gccgcacctt
    11461 tcgcggcgat cgatcgacct cgccagcgcg ctgggacggt cggcgcgtac cgcacgcgcg
    11521 gagtggcgcg acatcacctc cgccgcgccg ccgccgctct cgtgtgtcga gctctccggc
    11581 atcgtctaca cgaccgtgct gaatccgttc gaccagcgca agaactggga agacctgctc
    11641 acggcgtttc tcttcgcgct ccgcgaccac agcgatgcca cgctggtggt aaagctcgtc
    11701 gtcccgccgc aacgcgcggc gttcggcgtc aaccggatcg ttcgcttctg gcgcacgctc
    11761 gggatcgggc accggtgcag agtcgtcctc atcagcgcct atctcgacga cgcgcagatg
    11821 ctcgagctca ctggcgcaag cacgtactac ctcaatgcct cgcgcgccga aggagcgtgc
    11881 ctcccgctgc aggatttcct ggccgccggc cggccggccg tcgcgccgcg gcactccgcc
    11941 atcgccgact acttcgattc cggcagcggc ctggtcgtcg agtcgcaccc ggagccaacg
    12001 tcgtggccac aggatcctga tggccgctgc acaacgatat ggcatcggct cgtctggacg
    12061 tcgttgcgcg accgcatcgc cgagagttac gacatggcga cgcacgaccg cgccggctac
    12121 gcgcgcctct ccgcgaatgc tcaggcgcgc atgcacgcgc gggccagcga cgaggcggta
    12181 tgggcgcagc tcgagccggc gctcgactcg atcgagcaga gggaccggcg tgctctttaa
    12241 ctcattcgtg ttcatcggct tcttcgttgt cgtctacgcg gcgtacgtcg tgctgcggca
    12301 tcaagcacag aacgtgatgc tcctcctcgc aagctggttc ttttacggct cgtgggactg
    12361 gcgtttcctc ggcctgatgc tgttctccac agcgatggac tacgccttcg gccgcctgct
    12421 cgcgcgcgta caagatccgc ggcggaggaa gctgattctg gtcacgagcg tcaccatcaa
    12481 cctcggcatt ctcttcgtct tcaagtactt caacttcttc gccgcgacgc tcggcggcat
    12541 gctgcacgtc ttcgcgctcg atcccggcgc tccccttttg caggtcgtcc tgccgctcgg
    12601 catctcgttc tacacgttcc acaacatcag ctatatcgtc gacgtttacc atgagcgcgt
    12661 ggagccgacg cgcgacttcg tcgagttcgg cctctacgtc gcgctctttc cgcagctcat
    12721 cgccgggccg atcgcgcgtc ccgcgcacct gttgccgcag atccggacgc cgcgccgcat
    12781 cacgtacgag atgttccgca ccggcgcgtg gctcatcctc ctcggctatt tcaagaaagt
    12841 cgtgctcgcc gacaacatgg ccgcgttcac gaaaccgctg ttcgcgaatc cgcaggcgtt
    12901 tcatggcctg gaggtgctgt tcggccttta cgccttcgcc ttccagatct acggcgattt
    12961 ttccggctac tccgacatcg cccgcggtct cagcaagctg atgggattcg agctgatggt
    13021 gaacttccgc catccgtact tcgccaccaa ccccgccgac ttctggcgcc ggtggcacat
    13081 ctcgctctcg acctggctgc gcgattacct ctatatcccg ctcggcggca atcgcgaggg
    13141 gacgctgaag acgtaccgca atctgatgat cacgatgctt ctcggcggtc tgtggcacgg
    13201 cgcggcgtgg cacttcgtcg cctggggagc atttcatggc gtcctgctct gcgtccatcg
    13261 cgccgcggag ccgctgctgg cgtccttgcg ctcgatggcc gccgcgtcga gcgtattcgt
    13321 tcgccgcgtc gggcgcgctg gcgccgtctt cgcgttcttc aacgtcacct gcatcggatg
    13381 gcttctcttc gccgcgccga gcctgcgcga cgccggcacg ctgctgcgaa atacggtcgc
    13441 ccctgctctg acgcacggcc gcctcgtcgc cgtgacgatg ctctgcttcg cgctgccgct
    13501 gctggcgatg gagtgggcgc aggagcgaag cggcgccgcg gtccctgtcg tcaagacgtg
    13561 gccgcgcgcg ctgcggctgg cgtgctatat cgcggttttc tcagccattc tcctgtgcgg
    13621 tgcggtcgag cgccatgagt tcatctattt tcagttctga gaccgttcgc cgttacggcg
    13681 cgctccccag gaaggagcgt ttctggcacg cggcgatcgc cgtcgcggcc gcgatcgccg
    13741 tcctcgtctg ccgcgaaatc ccgccgcggc tctacgggac cagcggttgc cagggcggtt
    13801 cgctcggcag caccgcagtg gcgtcgctgt cggatcgcac ggaggtgttg ttcgccggat
    13861 cgtcgcacgt gctgttcggc gttcggccgc aacgtttcac ggttcacgcg atgaacctcg
    13921 ccggaacgtg gctcgactac acgtgcatcc gcgccgtcgt cgccaggcat ctccatcgcg
    13981 tcccgaacct gaaggtcgcg gtgatcgaat acgacgagct gccgtacgtt tcagatctcg
    14041 ttccggcgat tgtcgctacg cgagacgtcc ggccgctgca ggagctcggc ttgtcgcccg
    14101 cagagttccc gacggccggc gcgatgcagg cgggcgaggt tttcgccacg agcttcatgt
    14161 ttccgttcac cggtctgcct cgcgtcacgc cgctggcgtg gcgcaatcgg gacaagagct
    14221 gcagcccgct ctaccatccg ccgcgcggct tcgctcccgg ctactactac accgatgggg
    14281 tcacgccgaa gaacttcaac gccggcgccg tcttcaactc gctcgcgaag gcatcgcaaa
    14341 aagtgaacgt cgtccgcaga aacgcagcgg cgctcaccgc gaccatcgct ctgctgcgca
    14401 gccgcgggac gtccgtcgtg ctgctccggc ttccacacgc acgcgactac gtgacgcaac
    14461 gtccgccgat cgtgagcgcc cggtttcgcg agctgcagag gatcgccatc gccgccagcc
    14521 gctccgatgc cggcatcgcc gtgtgggatt tgggcgaaga tccggcgttc gctcccgccg
    14581 atttctgcga caacaaccac ctcaacgcgg cgggagcgga taagctggcg aaactgctcg
    14641 atgcgccgct ccgcgcgctc gcgtcgaaga ccgttgcgga ggtgccgtga agatcgtctt
    14701 gtgcaaaggc cgcttcgccg gcccgatcag cggcgccgac gaaacgatcg tcgcgtacgc
    14761 gatcgagctc agagcggcgg gacacgacgc ctgcgtcacc gttctccatc cgccgcgggc
    14821 cgacgacccg cactatcggc ggctgctcga cgcgcgcgtg ccggtgtcgt gcatcgccgg
    14881 ccggtcgctg gtcggccgtg gcatgcagtt cgttcgcaat cacatgccgc acattccgac
    14941 gggaccacgg cggctgttgc agaagacggc cgccggcctc tcctccagct acgatcgccg
    15001 ctgccacgcg tactttgcgc gcctgcagcc cgacgtcgtt cacgtcgtca cgcccgatcc
    15061 ggcgtccgtc gcgatcatcc gtggcgctca tgcggccaac gttcctgttc tgtaccagga
    15121 gctcggcacc gccgacttcc tccccgatct caatttctat tacgaccatc tcgccgcggc
    15181 actgccgctg gcgcacgtcg ccgctctctc tccgagcctc gcggcacgat tcggggagaa
    15241 gttcggtcga acggcggtcg acgtgctgcc catcaccctc gccgacgtcg ccgcgccggc
    15301 gccgaggtcg agcgacggcc gggtcacgtt cggcttcgca gcaaggctcg aatacggcaa
    15361 agggccgctt gccctggtcg acgcgttcgt aaaaaccgcg gatcgcgtgc ccgacgcgtc
    15421 gcttcgcatc gccggcggcg gtccgcagtt cgacgacgtg atgagtcgag ccgcggccct
    15481 cgcgctgaat gggcgctgca cattcaccgg cacgtacgcc ggaccggccg ctcgcgatgc
    15541 gttcatgcgc agcatcgacg ttctcgtgct ccccacgctg gccgaaggga cgccgaatac
    15601 gatcgtcgaa gcgatggcgc acgccgttcc ggtgatcgcc tcggctgtcg gcggcattcc
    15661 ggacatgctc ggatcggacg ccggggtgct cgtgcggcca gccgacatcg atgcgctcgc
    15721 tgcggcgatg accgagctcg gacgcgacgc cgcgcggcgt gaaatgctgg cgcgagcggc
    15781 gcgcgctcgt tacgaggagc tcttctcgcc agtcgcggtg gttccgctgc ttctcgccac
    15841 gtacgcgcga ctggccggcc acgacgacgc cgatcccgcg ccgcgccatc cctggtggaa
    15901 tggtcgctaa ccaaccctta aaatcgcctt aattttcctg ttttcggtga tcgctaaaaa
    15961 cagaaacggc cagcgtattc gccggccatt catatattta cattggttgc gggggcggga
    16021 tttgaacccg cgacctttgg gttatgagcc caacgagcta ccagactgct ccaccccgcg
    16081 acactggtga gaaacgctcg gcggccgatg ggaagcggct gcggcgaggg gcgcgattat
    16141 cggcgcgcgg ccctcatgcg tcaagggcct tcgtaaccga ggctttctcg gaagcattcc
    16201 aacggtgtcg ggagcttcgg gtcgaccttt acgatcaccc ttccgatcgg cgtttccggg
    16261 tagcggacat gacccgtcgc acacgcacga gcagcgttcc gggaacaggc tggcacggat
    16321 atcgtttgtc gcctcagcgg ccggatggat tgatcggccg ggcaatgagg accgacgaag
    16381 tcaccagaag atcctgagac ctgccaaaca gcgagacttc ctccacgaaa tgagacgatt
    16441 ccttttgatt ctgttgctgg cttcggcggc gcgtgcgacg acgctgccgg ggtttcatgt
    16501 tcaggttttg caggggacgc cggggtttct caccgcgctc gttgtggact cgcaggggac
    16561 gatttactac tcgaccaaga gtggggatgt tctgcgggtc gggcctgcgg agagagcctc
    16621 cgggaagccg gtcgtggtcg cgcatgtgac gaccgaggcg aatgggaact gtgggctgat
    16681 cgggttggcg ttgcgggacg acaacacggc catcgttcac tacacgacgc cggcgattac
    16741 gtacgacatc gtctcggcca ttgacctcac cactggcgct gagagccaga tccatcgctt
    16801 cgtctgcgac aaggatgtgg cggagcgggg tgtctccacc gagcaccacg ggggcaatct
    16861 cacggtggcg ccggatggct cgatctttct ggccatcggc gactatgggg ttggtagcct
    16921 cgctcaggat ccggcatgga acgcgggaaa gctctggcgg atcctgccgt acggttcggc
    16981 ggtgcagttc gcgcgcggct tccgcaatcc cttcgacctg gcgtgggatg cggcaacgca
    17041 gcgcgtcatc atcaccgaca atggcgacgc cgttgacgat gagatcgacg tggtggtgga
    17101 gggtgatgac tgcggctggc cgcttacggc agggaacagg ccgccggttg ccgggatgcg
    17161 tccgccggtc tacacgtttc cggtcgtggt cgcaccgacc gggctcgccg cgctcagcgg
    17221 acgcaatccc atcctccggc gcgggtatct gctgggcagc tttgtggcca aggctctctt
    17281 ctacgtcccc gacatctacg ccaatccgct ccccgatccg gtggcggtga ttcagaacga
    17341 aagcgatccg atcatcgacg tggccgaggg gccggacggg agcatctact tcgcgagcgg
    17401 cttcgtgatc tacaaactgg tcgtgccggc atcgggagac tgcaacggcg acggagtcgt
    17461 cgatgccgcg gatatctccg cgctgagcaa ggagttgtcg ggcggacctc acgctacgac
    17521 ggcagcgcac gcgagctggg gatgcgacgt caacggcgac ggcgtcatcg atgcgcgcga
    17581 tcttgcggcg ctggcgggaa tggtgcgggt gcgggcagtg cgaaggtgag gagagcgaca
    17641 accattcgcg ttctctccga gctcaaaacg aatcgtatac aaagtgacag tttggatgac
    17701 cgttcgctcg ctcttccgtt tgaccggtca gcctgcgcta aactgacccc atgaaacgac
    17761 tcctcatcgc gtccctcctc ctcatcgcct cggcggccca ggcaaaggaa gtgctgccgt
    17821 ggatcgagaa cgattacaag atggcgattg cccgcgcgaa ggcgacagac cggccggtct
    17881 tcgtcgaagc gtgggcaccg tggtgacaca cgtgccgctc gatgagggcc tatgtcttca
    17941 cggacaagtc cctcgagcgg tacgccggac ggttcgtctg gctctcgatc gatacggaaa
    18001 acgcaacgaa cgccgcattc ctgaaagcgc atccgatcag cgtctggccg acgctgctca
    18061 tcctcgaccc gaaagataag gtcgtcttcc gctacgccgg cggcggcacg gtgccgcaag
    18121 tccgcaagct gctcgatgac ggcgagcgcg ccgcgcaagg ctcgcacggc aaagcggata
    18181 cgttgttgat ccgcgccgat gcattggccg cgcaaggcaa acaggccgaa gccgccaagg
    18241 tgctcgacga ggcgatcgca gccgcgccga agaactggcc gcgcttcgga cgcgcgtcgg
    18301 agtcactcgt cttcacgctc ctgcaatcgc agaattcgca agcttgcgcc gagcgcgcac
    18361 tggcgctcta tccgcgcgtg cgcggtacgg gatcggccgt caacgtggcc ggtactggac
    18421 tctcctgcgc cagcgatctc gatccgaaga aatcgtggaa ccgttccgag ctcatcgccg
    18481 gtctggagaa agcgacgcgc gaaacgctcg ccgccgcaga caagatccac atgtcggccg
    18541 acgaccgctc cggtctctac gaatcgctcg ccgatgctcg cgatgaagcg aaagacgccg
    18601 cggcggcgca cgctctccgc gagcaatggg ctgcgttcct cgaaggcgaa gctgcgaaag
    18661 cgaagactcc cgatcagcgg gccgtctacg attcgcaccg cctcaccgct tacctcggcc
    18721 tgaaacaacc ggagaaggcc atcgccatgc tcgagcagtc ggagcgcgat ctgccgaacg
    18781 actacaaccc gccggcacgc ctcatctccg cgtacaaagc agccggccgt ttcgacgatg
    18841 ccctcgccgc gtccgaccgt gcgctgaagc tggcgtacgg ccctcgcaag atcggcatct
    18901 accgcaaccg cgcctcggtg tacgcagcca aaggcgacaa ggaatcagcg aaaaagacga
    18961 tcgaagaagc aatccgcttc gccgagtcac tgccaaccga gcaacaaagc gcgaacatga
    19021 tcgcgtcgat gaagaagcag ttgacgacga tgtgagcgtc ggggggtggg gagtggggtg
    19081 ttgggggtgg gagaacgaga ttagagacaa gcgatcagag atcagagact atcagagaca
    19141 agagatcagc gattcgtcag agatcagctc ggtgctctga tctctgtatc tctaatctct
    19201 gatctctaat aaatctctaa tctctgatcg ctcccactcc ccactcccca cttcccacac
    19261 cccactccca acctcacctc accgccctgc tgcgcggtgg aaacgtcggg gtgacgattg
    19321 cggcgttgct gtcgatcgag ctgatgcact cttcgctcac gcgcacccag tagctggcgg
    19381 tcacgctgac cgggttggtg atgaacgtgt cggagtcggt gccgactttc gttgagatat
    19441 cgcctttcac acccttgtac cactggtagt gcaacgggct gccggggatg ccggttccgg
    19501 tagcggtgtg cctggcctca atcttcaacg tggcgaccgt gctgacgccg gccgacaggg
    19561 acagtggctg cttgtcgatc gtcggcgagg ggcaggtcgc ggagagagtg acgacgccgg
    19621 tgacgctgtt gacgccgacg gtgttgcaac tgttcgccac atggacccaa taggccgtga
    19681 tggtcgtaac cggggtcacg gtgaccgagg aggtcgtggc gttgggaatc ggctgcgatg
    19741 tgttgcccga cgtgccggtg taccactggt aggtgatcgg gagcgtgccc gcggcggtga
    19801 ctgagagcgt tgtcgaagtg cccggaagga tcgtcacaaa ggacggatgg gtgatgatcg
    19861 agacctgggt gcaggtggtg tcggcgatga ggacgcctgt ggatgtggct gcggcaatag
    19921 tgacagcgag taacagtctg acgacgatgg gcattgagtt ttagcctttc ggggatcgcg
    19981 atttgaggtg ggatcttagc gtggtggaga gtgttaccgc ggtgatcacc agcgcgcgat
    20041 tgaaccaccg gggcggggac gccccgatgg ccgcgagcga gacgctcgca ctcctagtgg
    20101 cggacggcgc ggccgcgcgg aggaaagatc gccgtcaccg tagcggcgtt gctgtcggcg
    20161 gtgccgcact ggccggtgat gcgaacccag tactgattat tgaagtcgac cgcatccgtg
    20221 acgaaggtcg atgagtcggt gccgacggtc ttggtcgtgt tgcctttgtt gccctcgtac
    20281 cactggtagt gaagggccgt ccctgcggca acgaccttga acgttgcggc ggtgccgatc
    20341 ggggctgaga cgttcgctgg ctgcgtggtg atcgatgggg atgtacatgt cgataccgtg
    20401 atcgtggcgg tgttgctgtc ggccgtcgag ttgttgcagc cggtcacgcg cacccagaac
    20461 ttcgtcgtga cggtcggact ccggatcaat gaggccaagg tcgcgccctg gatggcgttg
    20521 gaggtatctc ccttgtcgcc ggtgaaccac tgataggtga acggggcggt tccagcggcc
    20581 accacggtca gcgtgaccgg tgaaccgctg gagatcgtcg tgcttgctgg ctgcgtcgcg
    20641 atcgaggcgg cgacacaggg cgacccattg acgacgacga ctacattgtt cgagtcctgc
    20701 gtgccacacg acgagctcac gcgcacccaa tagctggtgt tcaccgttgg agacacgacg
    20761 gtgctcgtac ctgtggcgcc gttgatcatg ttcgaggtat cgccggagct gccctggtac
    20821 cactgaatcg tgaatggacc gttccccgcc gtgttgattg agaggattga cgactggccg
    20881 ttattgatcg tctgcggcga agcgattggt cctgacgcga tcggcgcgca cgtcacggtc
    20941 accgtcgtct cccggctgtc ggacggcgaa ccgcactgtc cggtcacccg aacccagtag
    21001 gtggtggtcg tggtgggact tacggtgatg aacgagttgc tgctcggccc accgacgggg
    21061 ctcgtcgtca caccgaagtt tccgatgaac cactggtagg tcagcggacc ggtgccgctg
    21121 gcgctttcgg aaaggagact cgattgacca ttcgtgatgg aagagggaga ggcggaaggg
    21181 ggcgccgcgg ttgggggaac acacggagcg acggtcacgg tcacggctgg actgtccacc
    21241 gtcggtgtgc actgtccggt cacgcgcacc cagtacgagg tggtgaccgt cgggctgacg
    21301 gtgatggagt tgttgctcgt cgatccgccg atcggactcg tggtcacgcc cgagttgccg
    21361 ctataccact ggtaagtgaa cgggccgctg ccggtcggag tctcggtgat cgtgctcgat
    21421 tggccggacg tgatcgtaga tggattagcg accggagcgg gagcggacgg cggcgtgcag
    21481 gtcgtcacgg tgacggtcgc tgcattgctg ttcgccgagc cgccacacgc ggtcacgcga
    21541 acccaatagg tcgtagtcac ggtcggagaa acggtcaacg atgacgtcgt tccgttggat
    21601 gctgttgtgg tggtgctcgg aggattgccg atgtaccact gaaccgccgt attggtgccc
    21661 gtc
//
LOCUS       DATLVW010000856         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_405633, whole genome shotgun sequence.
ACCESSION   DATLVW010000856 DATLVW010000000
VERSION     DATLVW010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405633"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2730)
                     /locus_tag="VNN25_25050"
     CDS             complement(<1..2730)
                     /locus_tag="VNN25_25050"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXH94866.1"
                     /translation="MHKLAALCVRRPVFATVIVLSLVVVGWFAYQQLGVDRFPKVDIP
                     TITITTRLVGAAPEEIETDITDKVEEAVNTISGIDQLQSISSEGTSQVIVSFVLEKDI
                     DVAAQEVRDKVNTILRDLPKDADPPIILKIDPDAIPVLGIALSGPAPIRDITEFADKK
                     LRRRLESINGVGQVLIVGGRPRQINVVVDSAKLSAVGLTTAQLVAALQSQNVQIPGGK
                     VEQGLRDLTLRTYGRVQSPADFANIPLSTSNGYVVKVGDVAHIEDSVAEPESIATVNG
                     KPAVVMNIRKQSGTNTVEVIERLKEQLGHVRSDLPKGWTMQVVRDQSDFIVAAVDAVK
                     EHLILGSLFAALIVWFFLSSPKLREALIMLVSTLATYTLLFGVKDLAARAPAIHVSIL
                     TFIVLFALVSWLASKKQLPFAFRVGGAAVAFAIAAFVPGGGVIPINPLRLAGALVAFG
                     MLLYFFRRSRPTIIAAIAIPSSIIATFAAMRYEDFTLNVITLLALTLAVGIVIDDAVV
                     VLENIFRFMEEKKMSPDQAAVEGTKEVGMAVLATSLSLIAVFLPVAFMAGIVGRFMHS
                     FGVTMAIAIAVSLLVSFTLTPMMSARWLRREDLSHETSTREHGLYAHIESFYLKMLDW
                     SMAHRWVIVTAMVIALVSIVPLGILVNKNFLPSDDESQFQVQARAPEGASLQTTQTIM
                     ESIAARVRKLPEVETTVLTIGDDPQVTQNLGTVYVKLIPVDRRKRDQFAVMADVRNNI
                     MPQYQRLNLRTSVGPVNAFGGGVNAEIMYWIGGPDLNQLSKYSDTLLAQLRGMKSLGV
                     IDPDTNLITGKPELGVRIDRDKASDMGVRVQDIASTLNVLVGGLKITDYYEGGEQYEV
                     HVRAEHEYRKDPQGIAQAQVPSVGARTVQLRDVVTMSPGAGPASINRIQRQRQV"
     gene            complement(2784..>3692)
                     /locus_tag="VNN25_25055"
     CDS             complement(2784..>3692)
                     /locus_tag="VNN25_25055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXH94867.1"
                     /translation="TISILILAILPLGCEKKDAAPATTTQAAPALPGDVQNVASAATA
                     TTATTTASDPAGAASSGIASSESGGTIVATGELISPVRSELAVRIPGRVGKMYVDEGS
                     RVSRGQPLLEIETDYPRLNLDKANADIARAVSAEKDAQNDLNRKKELIAKESIPQATY
                     DRSQAMFDQARAAKQSAEAQAAIYRQQLSDSTLRSPIDGVVAEKRTDVGQRLGDATVA
                     FVVVQTSPLKLRFRVPEKELARVRLGQNVKATVDAYAGDVFQGKVAVVGGVIDPASRS
                     FTVETEFPNRDGKLRPGMFARVELGQ"
BASE COUNT          695 a         1109 c         1183 g          705 t
ORIGIN      
        1 gacctggcgc tgacgttgga tccggttaat cgacgccggc cccgcgccgg gcgacatcgt
       61 aacgacgtcg cggagctgca cggtacgcgc gccgacggac ggcacctgcg cctgcgcgat
      121 gccctgcggg tccttgcggt actcatgctc ggcgcgcacg tgcacttcgt attgctcgcc
      181 gccctcgtag taatcggtga tcttgagacc gccgacgagc acgttcaacg tcgaagcaat
      241 atcctgcacg cgcacaccca tatccgaggc cttgtcgcgg tcgatgcgga cgccgagctc
      301 gggcttgccg gtgatcagat tcgtatcggg atcgatgacg ccgagggact tcatgccgcg
      361 caattgtgcg agcagcgtgt ccgaatactt ggaaagctga ttgagatcgg gaccgccgat
      421 ccagtacatg atctccgcgt tgacgccacc gccgaatgcg ttgaccgggc cgacggacgt
      481 gcgcagattc agcctctgat actgcggcat gatgttgttg cggacgtcag ccatgaccgc
      541 gaactgatcg cgcttccgtc tgtcgaccgg aatgagtttg acgtagacgg tgccgaggtt
      601 ctgcgtgacc tgtggatcgt cgccgatggt caggacggtg gtctcgacct cgggcagctt
      661 gcggacgcgc gcggcgatcg attccatgat cgtctgcgtc gtctgcaaac tcgcaccctc
      721 gggagcgcgg gcctgcacct ggaactgcga ttcgtcgtcc gaggggagaa agttcttgtt
      781 cacgaggatg ccgagcggga cgatcgagac gagcgcgatc accatcgccg tgacgatcac
      841 ccagcgatgg gccatcgacc agtccagcat cttcaggtag aagctttcga tgtgcgcgta
      901 gagcccgtgc tcgcgggtgg aagtttcatg cgacagatcc tcgcggcgaa gccagcgcgc
      961 ggacatcatc ggcgtcagcg tgaacgaaac gagcaatgac acggcgatgg cgatggccat
     1021 cgtcacacca aacgagtgca tgaagcggcc gacgatgccg gccatgaacg cgaccgggag
     1081 aaagacggcg attagcgaaa gcgacgtggc caaaacggcc ataccgactt cctttgtccc
     1141 ttcgactgcc gcctggtcag gtgacatctt cttctcttcc atgaaccgga agatgttctc
     1201 gagcacgaca acggcgtcgt cgatgacgat gccgactgcg agagtcaacg ccagcagcgt
     1261 gatgacgttc agcgtgaagt cttcgtaccg catggccgcg aatgtggcga tgatcgagct
     1321 gggaatcgcg atcgcggcga tgatcgtcgg acgcgatcgc cggaagaagt agagcagcat
     1381 gccgaaggcg acgagtgcac cggcgagccg cagcgggttg atcggaatca cgccgccacc
     1441 tggcacgaat gccgcgatcg cgaacgccac ggcggccccg ccgacgcgga aagcgaacgg
     1501 cagttgtttc ttcgaggcca gccaactgac gagcgcgaac aacacgatga acgtcagaat
     1561 cgaaacgtga atggccggcg cgcgcgccgc gaggtccttc acgccgaaga gcagcgtgta
     1621 ggtcgccagc gtggacacca gcatgatcag cgcctcgcgc aatttcggcg acgagaggaa
     1681 gaaccagacg atgagcgcag cgaagagcga tccgagaatc agatgttcct tcacggcatc
     1741 gacagcagcg acgatgaagt cggactgatc gcggacgacc tgcatcgtcc atcctttcgg
     1801 aagatcggag cgcacgtggc cgagttgctc tttgagccgc tcgatgactt cgactgtgtt
     1861 cgtgccggac tgcttgcgga tgttcatgac gaccgccggc ttgccgttga cggtggcgat
     1921 cgactcaggc tcggccacgc tgtcttcgat gtgagcgacg tcgccgactt tcacgacgta
     1981 tccattcgac gtcgagagtg gaatgttcgc gaagtccgcg ggcgactgca cgcggccgta
     2041 cgtccttagc gtcagatcgc gaagaccctg ctcgaccttg ccgcccggaa tctgcacgtt
     2101 ctgcgattgc agcgctgcca cgagttgagc ggtggtcagg ccgaccgcgg agagtttcgc
     2161 cgaatcgacg acaacgttga tctgccgcgg tcgtccgccg acgatgagca cctggccaac
     2221 gccgttaatc gactccagcc ggcgtcgaag tttcttgtcg gcgaactcgg tgatatcgcg
     2281 gattggcgca ggaccggaga gcgcgatgcc gaggacggga atggcgtccg gatcgatctt
     2341 cagaatgatg ggaggatcgg cgtccttcgg caggtcgcgg aggatcgtgt tgaccttgtc
     2401 gcgcacttcc tgcgctgcca cgtcaatgtc cttctcgagg acgaagctga cgatgacctg
     2461 cgacgtcccc tcggacgaaa tggactgcaa ctgatcgata ccgctgatcg tgttcacggc
     2521 ctcttcgact ttgtcggtga tgtccgtctc gatctcttcc ggcgctgcgc cgacgagacg
     2581 cgtggtgatc gtgatcgtcg ggatgtcgac tttcgggaag cggtcgacgc cgagctgctg
     2641 gtaggcaaac catccgacga cgacgagcga gagaacgatg accgtcgcaa atacgggacg
     2701 gcgaacgcag agagccgcaa gtttatgcat ggtgttttcc cggttgctgg gttgctatgt
     2761 tgcctggttg gctggttgcc gattcactgt cccaactcca ctcttgcaaa catcccgggc
     2821 cgcaactttc cgtcgcgatt cgggaactct gtctcaactg tgaatgaacg cgaagccgga
     2881 tcgatcacgc cgccgaccac cgccactttg ccctggaaga catctcctgc gtaggcatcg
     2941 acagttgctt tcacgttctg tccgagtcgc acgcgcgcga gctctttctc cggaacgcgg
     3001 aagcgaagtt tgagcggcga ggtctggacg acgacaaaag cgacggtggc gtcgccgagg
     3061 cgctggccga cgtcggtgcg tttctcggcg accacgccgt cgatcggcga acggagcgtc
     3121 gagtcggaga gttgctggcg atagatcgca gcctgcgcct cggccgattg tttcgccgca
     3181 cgggcctgat cgaacatcgc ctgcgatcga tcgtacgtcg cttgcgggat cgactctttc
     3241 gcgatcaact ccttcttgcg gttcagatca ttctgcgcgt ctttctcggc ggacacggcg
     3301 cgcgcgatgt cggcgttcgc tttgtccaga ttgaggcgcg gatagtcggt ctcgatctcg
     3361 agcaatggct ggccgcggct gacgcgcgag ccttcgtcga catacatctt tccgacgcgg
     3421 cctggaatcc tgaccgcgag ctccgaacga accggcgaga tgagctcgcc cgtggcaacg
     3481 atcgtccctc ctgattcgga cgaggcgatg ccggaggatg ctgcaccggc aggatcgctt
     3541 gcggtcgtcg tagcggtcgt ggctgttgct gccgacgcga cattctgcac atcgcccggc
     3601 agcgccggcg cggcctgcgt cgtcgtcgcc ggggcagcgt ccttcttctc gcagccgagc
     3661 ggaaggatcg ccagaatcaa aatcgaaatc gt
//
LOCUS       DATLVW010000857        19094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_118750, whole genome shotgun sequence.
ACCESSION   DATLVW010000857 DATLVW010000000
VERSION     DATLVW010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118750"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VNN25_25060"
     CDS             complement(<1..1003)
                     /locus_tag="VNN25_25060"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH94868.1"
                     /translation="MPAAEIIATFRADTIAIVIAVVLCTMGAGMLLLGTLSARNARRS
                     FLYTGLFAIVYGVRLAFNTSSFALLSENPYWLPYLRSAFEYLVPIPAALLFKSFSEGR
                     RSALHRIVTAALAVCAAIAIPWEIATHSPYAFKPVIDALVLVLVAVLAIDLLAPGDEK
                     RNWRLVRIGALVFTAFVINEHFRFVGPDPYGLTREPTGFLFLMLTIIITTMREGTRAQ
                     QRLVAVDSELATARTIQLAALPRTNPELTGLEVSTIYMPASEVAGDFYDFVELENGTL
                     GVFIADVSGHGVPAALVASMLKIALATQIENASSPALILAALNTLFCGRLERQFITAA
                     "
     gene            complement(1146..3419)
                     /locus_tag="VNN25_25065"
     CDS             complement(1146..3419)
                     /locus_tag="VNN25_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXH94869.1"
                     /translation="MKRPIVALSVLFALAAVLQPLTAAASEAWILNIPVRGIPNQETG
                     MVRATLELDTPPAGSQLVINGSTTLNLGDTQIVAGDSVTYEALTGNNVRITYVPLSNF
                     GADFCVGNAAQDKNIPMRFVGAQDVVAYRMSTYIVAAPMAECSQVSKHTGDTPASLIP
                     NDDGVAQPLNAIYKGRNNFDVALVLDKSGSMADLPPGANAGPAKIDILKTAVQNFIGQ
                     WELLDAPPGGGPEWSGDRIGLVFFDSTAHPQTLVGADPPANFFLQRGNASAWDEIITA
                     ANALAPGSSTSIGGGVNEGMSQWKADPKNDLNMIVVTDGIQNTAPLITPTGSGFLGLT
                     PVAGLPQELRKRFVPIQTIAFGTPAQVDATLLTNMSFETSGVSFQALNATTMFDLWAK
                     TLVAILKGNTASMAVSVQDTLNGAGPSAAVPVIVDRSAQRAVFSVQWAPPSRRLLDLD
                     VFPPGAVTPAVPTSSKKTATASIQSFDIGRSFGPGKWTVRVKRDVKSAEPVPYALNVI
                     FLEKHLDYQFSLDNIHPVTGDPLGIHVLIDWDGKPLTGLPDGAIKVRVLRQPESMGTI
                     LHQTQREVQRGTIVTATGDIQTPLDAKIASFQGSSLLERITPREVAVLTLKDQGRGIY
                     AATFPDSTVAGRYVFEASLDWDTELTGHVVREETIEQNVKPRADRTKTDITINTTASG
                     ITTLTVTPRDHFGNYFGPGYASLIQARVRGNERTFVPDDQDQTGKYTFTIPGSPEVTP
                     IIDVIVDGVFVLGEDNR"
     gene            complement(3615..4043)
                     /locus_tag="VNN25_25070"
     CDS             complement(3615..4043)
                     /locus_tag="VNN25_25070"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HXH94870.1"
                     /translation="MSYAIDLNILLLASNAEAAEHEEARSFLELCAARPEVLCLTWPT
                     VMGYLRIATHPSIFSWPLSPLEALANIRSLLSLPQTRALGEQEGFLGIYESVAGTPAR
                     GNQVPDAHLAAILLQHGVTTLYTRDTDFRRFEFLELRKPF"
     gene            complement(4040..4300)
                     /locus_tag="VNN25_25075"
     CDS             complement(4040..4300)
                     /locus_tag="VNN25_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HXH94871.1"
                     /translation="MPRTTINLDGPVLADLKRLQRKEGKALGDLASELLASALADRKV
                     ATAPAVRLQWTSRPMGALLDLDDKEALFAALDKHPKKPRRRS"
     gene            complement(4385..5212)
                     /locus_tag="VNN25_25080"
     CDS             complement(4385..5212)
                     /locus_tag="VNN25_25080"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HXH94872.1"
                     /translation="MTETATSLKPCTNCGAPAAGIYCASCGERQPGHHDLSVAHFSHE
                     VFHEIAHVDSKLILTLRDLVAKPGFLTQEYFAGRKSRYIPALRLFLVLFALQFLAFTA
                     YEPAALYTVKSMKKFDRVGALHKLLDKKAQRIHVSAEELEQRIDERWHKNYSLLQLVN
                     IAALGLVLKVLYHRRYLAEHLVFAAHYLAFSYILSLVFIWPIFAVAGFQPGLTYKLTV
                     AATNGISLIYLFLAQRRFYGDSPALTALKTVFLMGGRLAIMVLLLGGSLIAAILMVH"
     gene            5415..5491
                     /locus_tag="VNN25_25085"
     tRNA            5415..5491
                     /locus_tag="VNN25_25085"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5449..5451,aa:Val,seq:gac)
     gene            complement(5502..6455)
                     /locus_tag="VNN25_25090"
     CDS             complement(5502..6455)
                     /locus_tag="VNN25_25090"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXH94873.1"
                     /translation="MRILVTGAAGFIATNLLERLLADGHEVHGIDNFFLGKREYVARS
                     LAKPGFTFHEFDLLDRDRVVALFQRLRPELVWHLAANSDISFGTSYTDFDLKGGTLVT
                     YNLLEAMRVAGSKQLIFSSSGSIYGEPTVLPTPENYGPLFPISFYAASKIACETLITA
                     FAAGYQMRCWIFRFGNIVGGYPTHGVIHDFVLKLRRDPTRLEILGDGTQSKPYVHVED
                     CLDGMMFGHRTATDLVNCYNLAVSDWTSVNQIAEWTIEAMGIDPKSVAITRTGGSRGW
                     PGDVPKVRLDTSRMTALGWTPKMGSDEAVRRSIRETVAQLA"
     gene            complement(6615..7307)
                     /locus_tag="VNN25_25095"
     CDS             complement(6615..7307)
                     /locus_tag="VNN25_25095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXH94874.1"
                     /translation="MNTQQVTIAILAGGFGTRLGNLTRDVPKPMIPIAGRPYLERVID
                     SFARCGLRDIVLLTGYRAEVVETHFGDGAAFGVRISYSRETEPLGTGGAIREARALLG
                     ERFVMTYGDVLRYFDYDRFVTAHDEPCLAVYPRESVGNAEVEGGRVIRFDKKAPELPF
                     IDAGFSIMPSSVIDLLPHHGPCSFEETIFPVLAAELLLAAEIVDLDFYDIGTPEELAR
                     TRAELEKQRSEE"
     gene            complement(7390..8052)
                     /locus_tag="VNN25_25100"
     CDS             complement(7390..8052)
                     /locus_tag="VNN25_25100"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH94875.1"
                     /translation="MKLTVSMITLNEERAVAKVVQDIRRVAPDAEIFLVDSSRDRTAE
                     IATEMGCRVFKQFPPKGYGPAMDRAVRDAAGDIVITLDCDDTYPVETIPILVALIDEG
                     YDLVNATRLATRPKAMPFANYLANRVFAWTARLLHGIRTTDVHSGMRAYRKSMIEKVQ
                     WNPNGPALPVDMLVIPYRMGFRIKEIPIEYRDRIGASTLQRWNSTKWTFKRLWRARRL
                     KR"
     gene            complement(8145..8888)
                     /locus_tag="VNN25_25105"
     CDS             complement(8145..8888)
                     /locus_tag="VNN25_25105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH94876.1"
                     /translation="MYESDSYQYFEDVNWGLLRLWHGRKNLDVLDVGCGFATTSQHIA
                     ALGNRVTGIESSAEAAAVARTRVNEIVHADLQQLDVVKSALAGRRFDVIIFADVLEHL
                     AWPIGILRGYLDLLKDGGSVIVSLPNVGLWSVRLNLLTGRFRYQETGVLDHTHLRFFT
                     RRTAREMIEQAGLKIVRRTYNPGLVRPFVPLAKKLLGGGAAEHDPSSLLESKPYKLYL
                     RTIYPVERAVASIWPGALAFQMIFEGGKK"
     gene            complement(8924..10249)
                     /locus_tag="VNN25_25110"
     CDS             complement(8924..10249)
                     /locus_tag="VNN25_25110"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH94877.1"
                     /translation="MTHHSIGILGGGISGVSLAAHLGEDVEVVEKRARIGGLCGTIVE
                     DGFTFDAAGPHIMFSKNKEVLNLMVAALGENVHQRRRENKIWFKGRLVKYPFENDLAA
                     LPPQDNFECIYGYIVNPHADDVPSNLAEWSYKTFGTGISEKYFIPYNEKIWNYPATKL
                     GLEFVERIPKPPMEDVLKSSIGISTEGYLHQLHFYYPIEGGYESLVHAFAAKVRGQLR
                     TNWRVDSVARDGNQWRVTSADGEERTYDTLVSTLPIHELLKVWTDAPDDVHRLVAPLR
                     YNSLINVLIGCNEDRGYPYTALYVPDPDLLFHRLSFPKAFSEHCVPAGSSSVMAEITT
                     NEGRDGIWEMTDEQIIERTIAELARTGLVDPSTIVYQKAVRFQYGYPVYDLDYRANVT
                     AMRNAVAATGLHLLGRFAQFDYINSDVCIERAIALAANLRSSASSADKT"
     gene            complement(10390..12306)
                     /locus_tag="VNN25_25115"
     CDS             complement(10390..12306)
                     /locus_tag="VNN25_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94878.1"
                     /translation="MKTERGTALLPIIALLAIAARLLQLQGLHPLVWDEIEFFRATDW
                     VWRGLVPYRDFWEHHTPLQWFLFAPVTALTNSPGASAIILMRWAQLPLWIAAFVLLRG
                     WMRRAGISSFAAASAATLVLSSTLFMLPAVEYRVDVLGCVIYIAAIACLQRLADGRRF
                     AFLGGAFLCLAGLANLRLGPLAVVTLLLVRVVRTRDRAWGGNTRANWSFAGAAAAFVP
                     AAVYFAATHSARMAWQRLWIDNFLADRFAPGVEWMFVHRIAAPFGIRLIDAVPNFQLS
                     LADPGGIAILVIGGVALVRALYRGFRAPGDEFFLAFLQVTNLLFIAAMKYVYNYHLEI
                     VALLMVPLAAIEIDRLVTSTARRRALLALIVVAAVVNIAAAVFRGKEADMQYEDFIMR
                     EADRRIPAGARVWDGAGWVLHHEPTYRYWFLRANVFVMEEHGYFEPYRVEDVLRDPPA
                     AVIADYTVDNWLALHRPFAGFILTHYLPLWREIWLPGMSARVTPATPAARWIVLADGT
                     YDVYASTNLASHPWFRDPFNFDHPALSGVHPPSAADRGARVDWLVDGAPIAPSNTLTL
                     RRGQRIDAVSHEPQPLGIMLIGAQNKAAFCQPTRGVKLEASEPPQWHIPDLAVLRQIA
                     RGTDAMPTREPCRR"
     gene            complement(12383..12649)
                     /locus_tag="VNN25_25120"
     CDS             complement(12383..12649)
                     /locus_tag="VNN25_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94879.1"
                     /translation="MIKKKDVQKAAKKAADTVIEASEEAYKKVSKAAAPKLKAMKKAA
                     APKVEQAKKAVKKQVAKGVKQAKSMTKDALMAGSKGLKRAAKKI"
     gene            12901..14433
                     /locus_tag="VNN25_25125"
     CDS             12901..14433
                     /locus_tag="VNN25_25125"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HXH94880.1"
                     /translation="MKLSLSHRLAGALTLAAAVLIVPLAHASSHREALAVLNEPCADN
                     TDTYAWVSNATHDKLYLVMDYNPLHEPGQGNQGFRSCNGYRYEFHIGKGTSLKDEAVY
                     RIEFKNTLTPSPAPGPTDALGGGNELLWQLTGGTETMTVTRIMAPGNPNPVVLGEAMT
                     VLPNNNGPQTDRLVYGLGAFKGYDSGDKTSRENGLYDQPFVDTFIHNLTGGGRIIAGD
                     FDDPYQLDEKGIFDLVNLGRSDLGGIQGARSTPIKDVFAGFNVSAIALEIPTSDLFPN
                     GIPHNGTLNAQSTDSLLRVWASISRQATQTVDPTNIITGVRGSGNYVQVGRNALPLFN
                     AGLVGTQRQTLYLHTDPMKDVTRFGNDILFPVLVRDAEALGIYKALGVPAAAVTTLKG
                     PRLDIINAINLGRPIPVADGSTGDVITLDAAIDSMFPNGRRIGGGSAPNRNQVNVNSV
                     LISLIVAGDPAAGLAKGVEVNDKNYLDRFPFLAPPHQGLLQGHGGTNAVTVPNIPDPN
                     NP"
     gene            14467..15228
                     /locus_tag="VNN25_25130"
     CDS             14467..15228
                     /locus_tag="VNN25_25130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94881.1"
                     /translation="MKRLASLLAITMAVITLPALAQARVPHAQGFASDFQTVPVMGNV
                     PGIGSTFQTYVAILNPTSSAYSIQASLFDASGVRHDAVITLAAGELKTYPNFLDAVFS
                     GFVGGGAVTFRSPDSAGGTRNNRFIIDTEVHTSGTRLGTSIPALEFAGTSSRSFSPGV
                     TVGAASRTNIGCFDQSGAVNSIKATVLDSTGTQTLGTQTLTLGANAWGQTALTTIVSN
                     GTVQFDPSDSAVCYAVVIDNISNDGRFVAASEYRP"
     gene            15322..16104
                     /locus_tag="VNN25_25135"
     CDS             15322..16104
                     /locus_tag="VNN25_25135"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HXH94882.1"
                     /translation="MRTTLTLVFAFIASTALAHDFWIEPSTFHPQVGTTLLTTLRVGQ
                     EFVGDPVARDSSMIERFILRDGAGDHDIAGIERRDPAGFLSVERPGIAVIGYRSRPKY
                     LEMPPDKFEQYLKDEGLESIVAFRAKRGDSMKPSREIFSRCAKALILADGNGTGARPD
                     APLSFRYEIVPKTNPYQMKGDEMQVAVLYEGKPLPNALVVAMHQEDSSLRIRLRSDAK
                     GRVTFHLPKRGVWLIKSVQMVPAPAGSNAEWESLWASLTFER"
     gene            complement(16699..17346)
                     /locus_tag="VNN25_25140"
     CDS             complement(16699..17346)
                     /locus_tag="VNN25_25140"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HXH94883.1"
                     /translation="MKRIVAAVVFLLLAGCASSKPADIVFLEGHLDLQPIPGSRSMRA
                     WSSRPAVLGDYHAFLFEPVVVILSNTAPDKQVSAEEMTDLGAHLRDAFKAELRRGGYS
                     VVDVPGAGVLRVRAALTELVPVDPTKNAAAKVVGAVIPGGLFLPTVDIGHAAIEVDMR
                     DCMTNARIAAFADRKAGRAYFNGLRPYRRWGDVENAFASWASEFRVRLDQVNGKK"
     gene            complement(17379..>19094)
                     /locus_tag="VNN25_25145"
     CDS             complement(17379..>19094)
                     /locus_tag="VNN25_25145"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXH94884.1"
                     /translation="LSAYAADEVVRLHALFDRTWEMNIKESPMFATSVGRHEYDDRLG
                     SSTMADLERRNAQEKATLAELKAIDRSKLPPEEMVNYDIFEEQIENGIASFELGDYQM
                     PFNADSGFHSGFARLAQNMPMATTKDYENYISRLNQWPRHVREEIALMRIGIARGMTV
                     ARATLDGYDSTITAHIVDDPEKSVFWKPFAKFPSTVPEGDRDRLRQAGRKAVMEGAVV
                     GYREFLDFFRNEYLPHARTTLGASELPNGRAYYAQQIREYTTLDLTPEQIHKIGLSEV
                     ERISGEMDAVMKEVGFKGDFPAFLVFLRTDPRFYAKTPEELLMRASRIAKRIDGKLPS
                     LFKTLPRLPYTVEPVPADIAPKYTSGRYVGPPEGSTQPGIYWVNTYMLESRPLYNLEA
                     LTLHEAVPGHHLQISLSRELTGLPNFRRFSYISAFGEGWGLYCEWLGLEAGIYSDQYS
                     NFGRLTYEMWRACRLVVDTGIHSMGWTRKQAIDYLASHTALPLHEVETEVDRYISWPG
                     QALSYKLGELKIKELRKRAEGTLGTRFDLRAFHDVVLGSGAVPLKVLEANVDRWIETQ
                     KAQAH"
BASE COUNT         3617 a         5577 c         6226 g         3674 t
ORIGIN      
        1 aggcggcggt gatgaactgg cgttcgagcc ggccgcagaa aagcgtgttg agcgcggcga
       61 ggatgagcgc gggcgatgac gcattttcga tctgcgtcgc cagcgcgatc ttgagcatcg
      121 aggcgacgag cgcggccggc acaccgtggc cggagacgtc ggcgatgaag acgccgaggg
      181 tgccgttctc gagctcgacg aaatcgtaga aatcgccggc gacttcggag gctggcatgt
      241 agatggtcga aacttcgagg ccggtcaact cggggttcgt gcgcggcagc gccgcgagtt
      301 ggatcgtacg cgcggtggcc agctcgctat cgaccgcgac gagccgttgc tgcgcgcgcg
      361 ttccttcacg catcgtcgtg atgatgatcg tcagcatcag gaagagaaag ccggtcggct
      421 cgcgcgtcaa gccgtaggga tcggggccga cgaagcgaaa gtgctcgttg atcacgaacg
      481 cagtgaagac gagggcgccg atacgcacga gccgccagtt ccgcttttcg tcgccgggcg
      541 cgaggaggtc gatcgccagc acggccacca gcaccaggac gagggcatcg atgaccggct
      601 tgaatgcgta cggcgaatga gtggcgatct cccacggaat ggcgatggcg gcgcagaccg
      661 cgagcgcggc ggtgacgatc cggtgcagcg ccgatcgtct gccctcactg aacgacttga
      721 acaacagcgc cgccggaatc ggcacgaggt actcgaacgc ggagcgcaga taggggagcc
      781 agtacgggtt ctccgagagc aaagcaaacg acgaggtgtt gaaggccagc cggactccat
      841 agacgatggc gaagaggcca gtgtagagaa aggaccgccg cgcgttgcgg gcgctgagcg
      901 tgccgaggag caacatcccc gcgcccatgg tgcagagcac aacggcgatg acgatcgcaa
      961 tcgtgtcggc acgaaaggtg gcgatgatct cggcggcggg catccaagag aagaataacc
     1021 acagagacac agaggtcaca gagaaaccgt atctctgtga cctctgtgtc tctgtggtgg
     1081 gaagcctttg tgtgcccgag acgtgcagcg atgcccattt cgggcatcgc tgcacggaaa
     1141 tcgacttacc ggttgtcctc gcccagaacg aagacgccgt cgacgatgac gtcgatgatc
     1201 ggggttactt ccggggagcc ggggatggtg aaggtgtatt tgccggtttg gtcctgatcg
     1261 tcaggaacga aagtccgctc gttgccgcgg acgcgggcct ggatcagcga tgcatagccg
     1321 ggtccgaagt agttgccgaa gtggtcgcgt ggggtgacgg tgagcgtcgt gatgcccgac
     1381 gcggtggtgt tgatcgtgat gtcggtcttc gtccggtcgg cgcgcggctt cacgttctgt
     1441 tcgatggtct cttcgcggac tacgtggccg gtgagctcgg tatcccagtc gagcgacgct
     1501 tcgaatacgt acctgccggc aacggtgctg tcagggaacg tggcggcgta gatccctctg
     1561 ccctgatcct tgagggtgag gacggcgact tcccgagggg tgatgcgctc gaggagggac
     1621 gatccctgga atgatgcgat cttggcgtcg agcggcgtct ggatatcgcc cgtcgccgtg
     1681 acaatggttc cgcgctgcac ttcgcgctgc gtctggtgca ggatcgtgcc catgctctcg
     1741 ggctgacgca gcacgcggac cttgatggct ccgtcgggga ggccggtgag cggcttgccg
     1801 tcccagtcga tcagcacatg gatgccgagc gggtcgccgg tgaccggatg gatattgtcg
     1861 agcgagaact gatagtcgag atgtttctcg aggaagatga cattgagcgc gtagggcacc
     1921 ggctcggcgc tcttaacgtc gcgcttcacg cgtaccgtcc atttgcctgg gccgaacgag
     1981 cggcctatgt cgaacgattg aatggacgcc gtcgccgttt tcttcgacga cgtcggcact
     2041 gcgggtgtca cagcgcccgg cgggaagaca tcgaggtcga gcagccggcg cgacgggggc
     2101 gcccactgca cggagaagac cgcgcgttgc gccgagcggt cgacgatgac cggaacggcc
     2161 gcgctcggcc ccgctccgtt cagcgtgtcc tgaaccgata cggccatcga tgcggtgttc
     2221 cctttaagga tggccaccag cgtcttcgcc cagagatcga acatcgtcgt ggcgttgagg
     2281 gcctggaagg aaacgccgga ggtctcgaac gacatgttgg tgagcagagt cgcgtccact
     2341 tgcgccggcg tcccgaaggc aatggtctga atggggacga aacgcttgcg cagttcctgc
     2401 ggcagtccgg ccaccggcgt caggccgagg aagccgctac cggtgggagt gatgaggggc
     2461 gccgtgttct gaatgccgtc ggtcacgacg atcatgttca aatcgttctt cgggtcggcc
     2521 ttccactggc tcatgccttc gttgacgccg ccgccgatcg aggttgagct tcccggtgcc
     2581 agcgcattgg cggcggtgat gatttcgtcc caggcgctgg cgttgccgcg ctggaggaag
     2641 aagttcgcgg gcggatcggc gccgacgagg gtctgcggat gcgcggtgga gtcgaagaag
     2701 acgagcccga tgcggtcacc cgaccattcc ggaccgccgc ccggaggcgc gtcgagcagt
     2761 tcccactggc cgatgaagtt ctgcacggcc gttttgagga tgtcaatctt tgccgggccg
     2821 gcgttggcgc ccggagggag gtcggccatg ctgccggact tgtcgaggac gagagcgaca
     2881 tcgaagttgt tgcggccttt gtagatggcg ttgagcggct gcgccacgcc gtcatcattc
     2941 ggaatcaggc tggccggcgt gtctccggtg tgcttcgaca cctgcgagca ttcggccatc
     3001 ggcgcggcga cgatgtacgt cgacatccgg tacgcgacga cgtcctgcgc gcccacgaag
     3061 cgcatcggga tgttcttgtc ctgggccgcg tttccgacgc agaagtccgc accgaagttg
     3121 gagagcggta cataggtgat gcggacgttg ttgccggtga gcgcctcgta ggtcaccgaa
     3181 tcgcccgcga cgatctgggt atcgccgagg ttgagggtgg tgctcccgtt gatgacgagc
     3241 tgcgagccgg ccggaggtgt gtccagctcg agagtggcgc gcaccatgcc cgtttcctgg
     3301 ttggggatcc cgcgtaccgg gatgttgagt atccatgcct cgctcgcggc ggctgtgagt
     3361 ggctgcagga cggcagccag agcgaagaga acagagagag cgacgattgg tcgtttcatg
     3421 agttgactcc tttttggtta aggggaaatc gaaatacgca aggttgggcg gaggcccgga
     3481 acgcatgcgt gtcgttatta atcggtcgca agctcggctg tttccgtgtt ccagatcaca
     3541 aggtgggggt tggagggatg caattgcggt tcccttcgtc ggaaaacgcc cccaagccaa
     3601 ttacgcggac tcgatcagaa gggtttgcgc agctcgagaa actcgaaccg gcgaaagtcg
     3661 gtgtctctgg tgtagagcgt ggtcacccca tgctgcagga ggattgccgc gaggtgcgca
     3721 tcgggaactt ggttgccgcg agccggcgtg cccgcgacgg attcgtagat accgaggaag
     3781 ccctcctgtt cgccaagggc tcgggtctga gggagggaga gcaggcttcg gatattggcg
     3841 agggcttcaa gtggggagag cggccacgaa aagatggagg gatgggtcgc aatccgcaag
     3901 tagcccatca ccgtcggcca agtaagacaa agtacctcgg ggcgcgcggc acacagttcg
     3961 agaaacgatc gtgcttcttc gtgctccgcg gcctcggcgt tcgaggccag gaggaggatg
     4021 ttgagatcga tcgcgtagct catgaccggc ggcgcggttt cttcggatgt ttatcgagtg
     4081 ccgcgaagag cgcctctttg tcgtcgagat cgagcagtgc gcccatcgga cgtgaggtcc
     4141 actgcagacg taccgccggt gcagttgcga ccttgcggtc cgcgagcgcc gacgccagta
     4201 gctcggaggc caggtcgccg agcgccttgc cttccttgcg ctggagccgt ttgaggtccg
     4261 cgagcaccgg gccgtcgaga ttgattgttg tgcgcggcat gatgcgtgat gctagggagc
     4321 gaagcatctg atgtcaaatg ctcgattgga gacaaaccgt cctctcccgt gctggccgga
     4381 gcgctcagtg caccatcagg atcgctgcga tgagcgagcc gccgaggagc agcaccatga
     4441 tcgcaaggcg gccgcccatc aggaacacgg tcttcaacgc ggtcaacgcc gggctgtcgc
     4501 cgtagaagcg gcgttgcgcg aggaagagat agatgagcga gatgccgttt gtagctgcga
     4561 ccgtcagttt gtatgtcagc ccgggctgga aaccagcgac ggcgaaaatg ggccagatga
     4621 agacgaggct gaggatgtac gagaaggcca gatagtgcgc cgcgaagacg aggtgctcgg
     4681 cgagataccg ccggtgatac agcaccttca gcacgagacc gagagcggcg atgttcacga
     4741 gctgcaggag cgagtagttc ttgtgccatc gttcgtcgat ccgctgctcg agctcctcgg
     4801 cgctgacgtg aatccgttgc gctttcttat cgagaagttt gtgcagggct cctactctgt
     4861 cgaacttctt catcgacttc accgtgtaaa gcgcggcggg ctcgtaggcc gtgaaggcga
     4921 ggaactgcag ggcgaagagg acgaggaaca agcgcaatgc cgggatgtac cgcgacttgc
     4981 gtcccgcgaa atactcctgc gtcagaaacc cgggtttcgc gaccagatcg cgcaacgtga
     5041 ggatgagctt cgagtcgacg tgagcaatct catggaagac ctcgtgggag aagtgagcga
     5101 cgctgaggtc gtgatggccg ggctggcgct cgccgcaact tgcgcagtag atgccggcag
     5161 ccggggcgcc gcagttcgtg cacggtttca gcgaggtggc cgtttcggtc attgatcgga
     5221 agtctagagg aagtccatcc gcagatgaca cagatgttcg cagtggacga gaacatctgt
     5281 gttcatctgc gacatctgcg gatcatccaa gccccgttgc atcggagccc ggattcgggc
     5341 tatcttccct ctcccgtcga ggggaatcat cacgggatcg gccctgttcg tgggctggtt
     5401 agcgagtctt ttacaggccc ttagctcagt tggttagagc accaccttga cacggtgggg
     5461 gtcagcggtt cgagtccgct agggcctacc aattctctga ctcacgcgag ctgcgccacc
     5521 gtctcccgga tcgagcgcct cacggcttcg tcgcttccca ttttcggggt ccaacccagc
     5581 gcggtcatgc gcgatgtgtc gaggcggacc ttcggtacgt cgccgggcca gccgcgcgag
     5641 ccgccggtgc gggtgatggc gacgctcttc gggtcgatgc ccatcgcttc aatcgtccac
     5701 tcggcgatct gattgaccga ggtccagtca gagacggcga ggttgtagca gttgacgaga
     5761 tctgttgccg tgcgatggcc gaacatcatg ccgtcgaggc aatcctctac atgtacgtac
     5821 ggcttcgact gcgtgccgtc gccgaggatt tcgaggcgcg tcggatcgcg ccgaagtttc
     5881 agaacgaaat cgtgaatcac accatgcgtc ggatagccgc cgacgatgtt tccgaagcgg
     5941 aagatccagc agcgcatttg atagccggcg gcaaaggcgg tgatcagcgt ctcgcatgcg
     6001 attttgctgg ccgcgtaaaa cgagatcggg aagagcgggc cgtagttttc gggagtgggc
     6061 agcaccgtcg gctcgccgta gatcgaaccg ctgctggaga agatgagctg cttcgagccg
     6121 gccacgcgca tcgcttcgag gaggttgtac gtgaccaacg tgccaccttt gaggtcgaag
     6181 tcggtgtagc tggtgccgaa gctgatgtcg ctgttggcgg cgagatgcca gaccagctcg
     6241 gggcgcagcc gctgaaatag cgcgacgacg cggtcgcggt cgagcagatc gaactcgtgg
     6301 aaggtgaagc cgggctttgc cagcgaccgg gcgacgtatt cgcgcttgcc gagaaagaag
     6361 ttgtcgatgc cgtggacctc gtgcccgtcg gcaagcagac gctcgaggag gttcgtggcg
     6421 atgaagccgg ccgcgccggt gacgaggatt cgcatggtgt gggtgtttcg tgattagtag
     6481 attagagatc agagactaga gatcagagac ttgagatcag gagcagggcc ctagagggtt
     6541 ccgagtccgt gacgcgcgtt acgatatcag ttgcggtgtc gacggttcgc gatcggcgct
     6601 ctgatctctt atctctactc ctctgatctc tgtttttcca actccgcacg cgtccgcgcc
     6661 agttcctcgg gcgtgccgat atcgtagaaa tcgaggtcga cgatctcggc ggccaggagg
     6721 agttctgcgg caagaactgg aaagatcgtc tcctcgaacg agcaggggcc gtgatgggga
     6781 aggagatcga tcacgctcga cggcatgatc gaaaagccgg cgtcgatgaa gggcagttcc
     6841 ggcgctttct tgtcgaagcg gatcacccgt ccgccttcga cctcggcgtt cccgacgctc
     6901 tcgcgcggat aaacggccag acatggctcg tcgtgcgcgg tcacgaatcg atcgtagtca
     6961 aagtagcgaa ggacgtcgcc gtaggtcatc acgaagcgct cgccgaggag cgcgcgggct
     7021 tcgcgaatcg cgccgccggt gccgaggggc tcggtctcac ggctgtagct gatgcgaaca
     7081 ccgaatgccg ctccgtctcc aaagtgcgtc tcgactacct cagcgcgata accggtgagg
     7141 agcacgatgt cgcgcaatcc gcatcgcgca aacgaatcga tcacgcgctc gagatacggg
     7201 cggccggcaa tcgggatcat cggctttggt acgtcgcggg tgaggttgcc gaggcgcgtg
     7261 ccgaagccgc cggcgaggat ggctattgtg acttgttgtg tgttcatgaa ttcagattca
     7321 gaagcaccac agagacacag aggacacaga gaaatctctg tgctctctgt gtctctgtgg
     7381 tgaaaaaact catcgtttaa gccgccgcgc gcgccacaac cgcttgaacg tccacttcgt
     7441 cgagttccag cgttgcaatg tcgacgcgcc gatgcggtcg cggtattcga tcgggatctc
     7501 tttgatgcgg aagcccatgc gatacgggat cacgagcatg tcgacgggca gcgcggggcc
     7561 gttcggattc cactgcactt tctcgatcat cgatttgcgg taggcgcgca tgccgctgtg
     7621 gacgtcagtc gtgcggatgc catgcagcag ccgcgcggtc catgcgaata cgcggttggc
     7681 gaggtagttc gcgaacggca tcgcttttgg acgcgtggcc agacgagtgg cgttgacgag
     7741 gtcgtagcct tcatcgatca gcgccacgag aatcggaatc gtttcgacgg gataggtgtc
     7801 atcgcaatcg agtgtgatga cgatgtcgcc ggcagcgtcg cgcacggcgc ggtccatcgc
     7861 cggtccgtag cctttcggag ggaactgctt gaagacgcgg caacccatct ccgtggcaat
     7921 ttcggcggta cggtcgcggc tggagtcgac gagaaagatc tccgcatcgg gcgcgacgcg
     7981 gcggatgtcc tgaacgacct tggcaacggc gcgctcttcg ttgagcgtaa tcatggagac
     8041 ggtgagtttc atggattcag agatcagaga cacaagagat cagagatcag gaaacggtgc
     8101 tcgcagtttg cgggatgtcc tgatgtctga tctcttatcg ctgatcactt cttcccccct
     8161 tcgaaaatca tttgaaatgc gagcgcgccc ggccagatgc tggcaaccgc gcgctcgaca
     8221 gggtagatcg ttcttagata cagtttgtac ggcttcgatt cgagaagcga cgaaggatcg
     8281 tgctccgccg cgccgccgcc gaggagcttc tttgccagtg ggacgaacgg gcgcacgagg
     8341 ccgggattgt atgtgcggcg aacgatcttc agtccggctt gctcgatcat ctctctcgcc
     8401 gtgcggcgcg tgaaaaatcg aaggtgagtg tgatcgagga cgcccgtctc ctggtagcga
     8461 aaccgtccgg tgagcagatt gagccgcacg gaccagaggc cgacattcgg cagcgagacg
     8521 atgacgctgc cgccgtcttt gagcaggtcg agataaccgc gaaggatgcc gatgggccac
     8581 gccagatgtt cgaggacgtc tgcgaagatg atgacgtcga agcgccggcc tgcgagagcg
     8641 ctctttacga cgtcgagttg ctgcaggtcg gcgtggacga tttcgtttac gcgtgtacgt
     8701 gcgacggcgg ccgcctccgc gcttgactcg atccccgtca cgcggttgcc gagcgcagcg
     8761 atgtgctgcg aggttgtggc aaagccgcag cccacatcga gaacatcgag attcttgcgg
     8821 ccgtgccaaa gacgcaataa gccccaattg acgtcttcga aatattgata gctatcggat
     8881 tcgtacattg gttttatctg tgtcgatctg cgtcttctgc ggatcaggtt ttgtccgcag
     8941 atgacgcaga tgaacgcaga ttcgccgcca gtgcaatcgc gcgctcaatg caaacgtcgc
     9001 tgttgatgta atcgaactgc gcaaagcgtc cgagcagatg caaaccggtg gcggcgaccg
     9061 cgttgcgcat ggcggtgacg ttggcgcggt agtcgagatc gtagaccgga tagccgtact
     9121 gaaaacgaac cgccttttgg tagacgatcg tcgatgggtc gacgaggccg gtgcgggcga
     9181 gctcggcgat cgtccgctcg atgatctgct cgtccgtcat ctcccatatg ccgtcgcgtc
     9241 cttcgttggt cgtaatttcg gccatgacgg acgaggaacc ggctggcacg caatgctcgc
     9301 tgaaggcttt cgggaacgag aggcggtgaa agaggaggtc gggatcgggg acgtagagcg
     9361 ccgtgtaggg gtagccgcgg tcctcgttgc agccgatgag aacattgatc agcgagttgt
     9421 agcgcagcgg cgcgacgaga cgatgcacgt cgtcgggcgc gtcggtccac actttcaaca
     9481 attcatgaat cggcagcgtg gagacgagcg tgtcgtacgt tcgctcttcg ccatcggctg
     9541 aagtcacccg ccactggttg ccgtcgcgcg cgaccgagtc tacgcgccag ttcgttcgga
     9601 gctggccgcg cactttcgca gcgaacgcgt gaacgagcga ctcgtaccca ccctcgatcg
     9661 gatagtagaa atgcaactgg tgcaaatacc cctcggtcga aatgccgatc gacgacttga
     9721 gtacgtcttc catcggcggc ttggggatcc gttcgacgaa ttccagcccg agcttcgtgg
     9781 cgggatagtt ccagatcttc tcgttgtacg ggatgaagta cttctccgag atgccggtgc
     9841 cgaacgtctt atacgaccac tcggcgaggt tcgacggcac gtcatccgcg tgcggattga
     9901 cgatgtagcc gtagatgcat tcgaagttgt cctgcggcgg aagcgcggcg aggtcgttct
     9961 cgaacggata cttcaccagc cgtcctttga accagatctt gttctcgcgg cggcgctggt
    10021 gaacgttctc gccgagcgcg gcgaccatca ggttcagcac ctctttgttc ttgctgaaca
    10081 tgatgtgcgg ccccgcggcg tcgaaggtga agccgtcttc gacgatcgtg ccgcacaggc
    10141 cgccgatgcg ggcgcgtttc tcgacgacct cgacgtcttc gccgaggtgt gccgcaaggg
    10201 agacgccgga gatgccgccg ccgaggatgc cgatggagtg atgggtcatg aggtgccggg
    10261 ccgggtattg tagtcggggt ggcgggttct cgcggttgaa ggacggcccc tcatccggcc
    10321 ttcggccacc ttctccccat caaaaggcga tggggagaag gggctcgatt gggaggtgag
    10381 gcaattctct catcgccggc acggctcgcg cgttggcatc gcatcggttc ctctcgcgat
    10441 ctggcgcagt acggctaaat cggggatgtg ccattgcggc ggttcggagg cttcgagttt
    10501 gactccgcgc gtgggctggc aaaacgctgc tttattttga gcgccgatga gcatgatgcc
    10561 gaggggttgc ggttcgtgag ataccgcgtc gatgcgctgg ccgcggcgga gtgtgagcgt
    10621 attggatggc gcgatggggg cgccgtcgac gagccagtcg actcgagctc cacgatcggc
    10681 ggccgatggc ggatggaccc ccgacaacgc gggatgatcg aagttgaacg gatcgcgaaa
    10741 ccaggggtgc gatgcgaggt tcgttgatgc atagacgtcg taggtgccat cagcaaggac
    10801 gatccagcgc gccgcgggag tggccggcgt gacgcgggcg ctcatgcccg gcagccagat
    10861 ctcgcgccag agcgggaggt agtgagtgag gatgaatccg gcgaacggac ggtgcagcgc
    10921 cagccagttg tccacggtgt agtcagcgat gacggcagcg ggcgggtcac gcaagacatc
    10981 ctcgactcga tacggctcaa aatagccgtg ctcctccatg acgaaaacgt tcgcgcgcag
    11041 aaaccagtag cgatacgtcg gctcgtggtg cagcacccag ccggcaccgt cccagactct
    11101 ggcgccggca ggtatgcggc gatcggcctc gcgcatgatg aagtcctcgt actgcatgtc
    11161 cgcctctttg ccgcggaaga ctgcggccgc gatgttgacc acggcggcga cgacgatcag
    11221 tgcgaggaga gcgcggcgtc gcgcagtcga cgtgaccaat cgatcgatct cgatggcggc
    11281 gagcgggacc atcagcaggg cgacgatctc gaggtggtag ttgtagacgt acttcatcgc
    11341 ggcgatgaag aggagattcg taacctgcag aaaggccagg aagaattcgt cgcccggcgc
    11401 gcggaaaccg cggtagagcg cgcggacgag agcaactccg ccgatgacca gaatggctat
    11461 gccgcccgga tcggcaaggg agagttgaaa attcggaacg gcatcgatga ggcggatacc
    11521 gaacggcgcg gcgatgcgat gaacaaacat ccactcgacg cccggcgcga atcgatctgc
    11581 cagaaagttg tcgatccaga gccgctgcca cgccatgcgc gccgaatggg ttgccgcgaa
    11641 gtagaccgcg gccggaacga aggccgcggc ggcgccggcg aagctccagt tcgcgcgcgt
    11701 gttaccgccc caggcgcggt cgcgcgtgcg cacgacgcgc acgagcagca gtgtgacgac
    11761 tgccagcggg ccgaggcgaa gattggcgag gccggccagg caaaggaaag cgccgccgag
    11821 gaaggcgaag cggcgtccgt cggcaaggcg ctggagacat gcgatggcgg cgatgtagat
    11881 cacacagccg agcacgtcga cccggtactc gaccgcaggg agcatgaaga gcgtgctgga
    11941 gaggacgagc gtcgccgcgc tcgcagccgc gaaactggag atgcccgcgc ggcgcatcca
    12001 gccacgcagc aggacgaacg cggcgatcca gagcggcagt tgcgcccagc gcatcaggat
    12061 gatcgcgctc gctccggggc tgttcgtcag cgccgtcacc ggcgcgaaga ggaaccactg
    12121 caacggagtg tggtgctccc agaaatcgcg ataggggacc aggccacgcc acacccagtc
    12181 cgtcgcgcgg aagaactcga tctcatccca gacgagcgga tggagtccct gtagctgcag
    12241 caaccgggcg gcgatggcca ggagcgcgat gatcgggaga agcgcggttc cgcgctctgt
    12301 tttcattggc gcgggatgag gtcaaagccc gaagggggcg cggcagacgc gcgccccgac
    12361 gggtcgttcc gctgtggagt ggtcagatct tctttgctgc gcgcttcaag cctttcgagc
    12421 cggccatgag cgcgtctttg gtcatcgact tggcctgctt cacgcctttg gcgacctgct
    12481 tcttgacggc cttcttagcc tgctcgacct tcggtgcagc cgccttcttc atcgccttca
    12541 gcttgggagc cgcagctttg ctgacctttt tgtacgcttc ctcgctcgcc tcgatgacgg
    12601 tgtccgctgc tttcttcgca gccttctgaa cgtctttctt cttgatcatg ggagtgccct
    12661 cctttgttga tggttcgcta ccttagcact gccctgttcc aatccggaag cgcgggtcct
    12721 tgcaacggaa gaaaagcgtt tcacgcggat gcgcggagtc cacggagaaa cccccgcgtc
    12781 ctccgcgtct ccgcgtgaga gggttagccc ttttttcttc gctccctaaa tctgctttga
    12841 ctttcgcaac gtaaaccttc tcgaaccacg gaaacggatc cgtggagcaa aggagaaccg
    12901 atgaaactct cgctctcgca ccggctggct ggtgcactca ctctcgccgc tgctgtattg
    12961 atcgttccgc tggcacacgc ttccagccat cgcgaagcgc tggccgttct caatgagccg
    13021 tgcgcggaca acaccgacac ctacgcctgg gtgtcgaacg cgacgcacga caagctgtac
    13081 ctggtcatgg actacaaccc gctccacgag cctggtcagg gcaatcaggg atttcgttcc
    13141 tgcaacggat accgatacga gttccacatc gggaaaggaa caagcctcaa ggacgaggcc
    13201 gtctatcgca tcgagttcaa gaatacgctg actccctcgc ccgctcccgg tccgacggat
    13261 gcgctcggcg gtggcaacga gcttctctgg cagttgaccg gcggtaccga gaccatgacg
    13321 gtcacgcgga tcatggctcc cggcaacccc aacccggtcg ttttgggcga ggccatgacc
    13381 gttctgccga acaacaacgg tccgcagacc gaccggctcg tttatggact cggcgctttc
    13441 aagggctacg attccggcga taaaacgagc cgcgaaaacg gtctttatga ccagccgttc
    13501 gtcgatacgt tcatccacaa tctgaccggc ggtggccgca tcatcgcggg cgatttcgac
    13561 gatccatatc aactcgacga gaaaggcatc ttcgacctcg tcaatctcgg ccgcagcgat
    13621 cttggaggta tccagggagc gaggagcacg cccatcaagg acgtcttcgc gggcttcaat
    13681 gtctctgcga tcgcgctgga gattccaacg tcggatttgt ttcctaatgg catcccgcac
    13741 aacggaacgc tcaacgcgca atccaccgat agccttctgc gcgtctgggc cagcatcagc
    13801 cgtcaggcaa cgcagaccgt cgatccgacc aacatcatca ccggcgttcg cggttccggc
    13861 aactatgtac aagtggggcg caatgcgctg ccgctcttca acgccgggct ggtaggcacg
    13921 cagcggcaaa cgctctatct gcacacggac ccgatgaaag acgtgacccg gttcgggaat
    13981 gacattctgt tccccgttct tgttcgcgac gccgaggcgc tgggcattta taaggcgttg
    14041 ggcgttcctg cggccgcggt taccacactg aaaggaccgc ggcttgacat catcaacgcc
    14101 atcaacctcg gccggccgat tcctgtcgcc gatggatcca ctggcgacgt catcaccctc
    14161 gatgcggcaa tcgattcgat gttcccgaac ggacgacgga tcggcggcgg gtcggctccc
    14221 aatcgcaatc aggtgaacgt caacagcgtg ttgatcagtc tgatcgtcgc cggggatcca
    14281 gctgcgggtc tggccaaagg tgtcgaagtg aacgataaga attatctcga ccggttcccg
    14341 ttcctggcgc cgcctcatca gggcttgctc cagggacatg gcggcacgaa cgccgtaacc
    14401 gttccgaaca tcccggatcc gaacaatccg tagtaacacg cagacaaaca tcaacaggag
    14461 atcatcatga agagacttgc atctctcctc gccatcacaa tggctgtcat tacattgccg
    14521 gccctcgccc aagcacgcgt tccgcacgcg cagggttttg catccgattt tcagacagta
    14581 ccggtcatgg gcaacgtgcc cggaatcggt tcgacgtttc agacgtatgt ggcgatcctg
    14641 aatccgacat cgtctgcgta ctcgatccag gcttcgctct ttgacgcgtc gggcgtcagg
    14701 cacgacgccg tgatcacgct cgccgccggc gagttgaaga cctacccgaa ctttctcgat
    14761 gcagtgttct cgggttttgt cggcggcggc gcggtgacgt tccggagtcc cgactccgcg
    14821 ggcggcacgc gcaacaaccg cttcatcatc gacaccgagg ttcacacgag cggtacgcgg
    14881 ctgggcacgt cgattccggc gctcgagttc gcggggacga gctcgcggtc gttcagcccg
    14941 ggagtgactg tcggcgcggc atcgcggacg aatatcggat gcttcgatca gtcgggcgcc
    15001 gtgaattcga tcaaagcgac cgtcctcgat agcaccggaa cgcagaccct cgggacgcag
    15061 acgctcactc tcggcgcgaa cgcctgggga cagactgcgc tcacgacgat cgtcagcaac
    15121 ggtactgtgc agttcgatcc gtcggactcc gcggtttgct atgccgtggt catcgacaat
    15181 atttcgaatg acgggcggtt cgtagcggct tcggagtatc ggccgtaatc gctcagaatc
    15241 aatgaaccac ggaggcacag agggcacaga gggagactct gtgtcactgt gtctctgtgg
    15301 tgccggtttg ttagcaacct tatgcgaacc actctcacac tcgtctttgc attcatcgcc
    15361 agcaccgcac tcgcccacga cttctggatc gagccgtcga cgtttcatcc gcaggtcggg
    15421 acgactctgt tgacgacgct ccgggtcggt caggaattcg tcggcgatcc tgtggcgcgc
    15481 gattcgtcga tgatcgagcg gttcattctt cgcgacggtg ccggcgatca cgacatcgcc
    15541 ggcatcgagc gcagggaccc agcgggcttt ctttctgtcg aacgtccagg catcgcggtg
    15601 atcggctatc gaagccgccc gaaatacctg gaaatgcctc cggataagtt cgagcagtac
    15661 ctcaaggacg aggggctcga atcgatcgtc gcctttcgcg cgaagcgggg cgattccatg
    15721 aagccttcgc gagaaatctt ctcgcgctgc gccaaggcac tgattctcgc tgacggcaac
    15781 ggaacgggcg cgcgccccga cgcgcccctg tcgttccgct acgagatcgt cccgaagacc
    15841 aatccgtacc agatgaaggg cgacgagatg caggtggccg ttctctacga agggaagccg
    15901 ctgccgaacg cgctggtcgt ggccatgcac caggaagatt cgtcgctgcg aatccgcctc
    15961 cgctccgatg ccaaaggccg cgtcaccttc cacctcccga agcgcggcgt ctggctgatc
    16021 aaatccgtgc aaatggtccc cgccccagcc ggctcgaatg cggagtggga aagcctttgg
    16081 gcgtcgctga cgtttgagag gtgagggagt ggggagtggg gggtggggtg tggaagaaga
    16141 gattctggaa ccacgccgtg tcgaggactt cttagttatt cgcaggtaac aagcaaaaga
    16201 taccgaaggc ctgaccccct cgccagtccg acattgggga cgctcccttt cgcattggaa
    16261 acgctccgtt tgacattagc tgcgccccgt tcgacattag cgccgctccg tttgacatta
    16321 gccgcgcgcc gtttgacatt agcgccgcgc cgtttgacat tagccgcacg ccgtttgaca
    16381 ttagccgcgc tccgttcgac attagcgccg cgccgtttga cattagccgc gcgccgtttg
    16441 acattagccg cgcgccgttt gacattagcg gcgctccatt cgacattagc cgcgccccgt
    16501 ttgacattag ccgcgctccg tttcacatcg gaggcgcgtc cttcgattac gatgccctgt
    16561 cgatatcgag cctggagact cgaatatcga gagcacgctt ccagatccgc agatgacgca
    16621 gatcgacgca gatcaaaccc tcatctgcgt ttatctgcgt catctgcgga tgagcattcg
    16681 tccctccgac tcccaaacct acttcttccc attcacctga tcgagccgta cccgaaactc
    16741 gctggcccag gaggcgaacg cgttctcgac gtcaccccag cgacggtacg ggcgcagacc
    16801 gttgaagtat gcgcggccgg ctttgcggtc ggcgaatgcg gcgatgcgcg cgttggtcat
    16861 gcagtcccgc atgtccactt cgatcgcggc gtgaccgatg tcgaccgtcg gcaggaacag
    16921 gcctcccggg atgactgcgc caacgacttt cgcggcggca ttcttcgtcg gatcgaccgg
    16981 cacgagctcg gtaagcgctg cgcgaacgcg aagcacaccg gcgcccggaa catcgaccac
    17041 ggaataccct ccgcgccgca actcggcctt gaaagcatcg cgaaggtgcg cgccgaggtc
    17101 cgtcatctcc tccgcgctga cctgtttgtc gggcgccgtg ttactcagga tgaccactac
    17161 cggctcaaaa agaaacgcgt gatagtcgcc gagaacggcg gggcggctgg accaggcgcg
    17221 catcgaacgg cttccgggga tcggctgcag atcgaggtgg ccttccagaa agacaatgtc
    17281 cgccggtttc gacgacgcgc atccggcgag gagcagaaac acgacagcgg ccacgattct
    17341 cttcatcgaa ttctcctgac gatccatcaa tcggacgatc aatgtgcttg cgccttctgc
    17401 gtctcgatcc atcgatcgac gttcgcttcg agcactttca acggcaccgc gccgctgcct
    17461 agtacgacgt cgtgaaaggc gcgtaagtcg aagcgcgtgc cgagcgttcc ctcggctcgc
    17521 ttgcggagct ctttgatttt cagctccccg agcttgtacg agagcgcctg gcccggccag
    17581 gagatgtagc gatcgacttc ggtctcgact tcgtgcagcg gaagcgcggt atggctagcc
    17641 agatagtcga tggcctgttt gcgcgtccag cccatcgaat gaatgccggt atcgacgacc
    17701 agccggcagg cgcgccacat ctcatacgtc aggcgtccga agttcgagta ctgatccgag
    17761 tagatgcccg cttcgagtcc cagccactcg cagtagaggc cccacccttc gccgaacgcg
    17821 gagatgtacg agaagcggcg gaagttcggc aggccggtca gctcgcggct cagggagatc
    17881 tggaggtgat ggcccggcac cgcttcgtga agtgtgagcg cctcgaggtt gtagagcgga
    17941 cggctttcta gcatgtaggt gttcacccag tagatgcccg gctgcgtgct cccctcgggc
    18001 ggcccgacgt agcggccgct cgtgtacttc ggggcgatat ccgccggcac cggctcgacc
    18061 gtgtagggta gccgagggag ggtcttgaag agcgagggaa gctttccgtc gatgcgtttg
    18121 gcgatgcgcg atgcgcgcat cagcagctct tcgggcgtct tcgcgtagaa gcgcggatcg
    18181 gttcgcagga aaacgaggaa ggcgggaaag tcgcccttga agccgacctc cttcatcacc
    18241 gcatccattt cgcccgaaat gcgctccact tcgctgagtc cgatcttatg gatctgctcc
    18301 ggagtcagat cgagcgtcgt gtactcgcgg atctgctgcg cgtagtaggc ccgcccgttg
    18361 ggaagctcgg aggcgccgag ggtggtgcgg gcgtgaggga ggtactcgtt gcggaagaaa
    18421 tcgaggaact cgcggtaccc gacgaccgcg ccttccatga ccgctttgcg ccccgcctgc
    18481 cgcaaccgat cgcgatcgcc ctcggggacc gtcgacggga acttcgcaaa cggcttccag
    18541 aagacgctct tctcaggatc gtcgacgata tgagcggtga tcgtcgagtc gtatccatcc
    18601 agagtcgcgc gcgcaaccgt catgccacgc gcgatgccga tgcgcatcag cgcgatttcc
    18661 tcgcgtacgt ggcgcggcca ctggttcagg cggctgatgt agttctcgta gtccttcgtc
    18721 gtcgccatcg gcatgttctg ggcgagccgc gcgaatccgg agtggaatcc cgagtcggca
    18781 ttgaacggca tctggtaatc gccgagctcg aaactggcga tcccgttctc gatctgctcc
    18841 tcgaagatgt cgtagttgac catctcctcg ggtggcagct tcgaccggtc gattgccttc
    18901 aactcggcga gcgtcgcttt ctcctgcgca ttgcgccgct cgagatcggc catcgtcgac
    18961 gagcccaacc ggtcgtcgta ctcatgccgc ccgaccgacg tcgcgaacat cggactctct
    19021 ttgatgttca tctcccacgt gcggtcgaag agcgcatgaa gacggacaac ttcatcggct
    19081 gcgtatgcgg aaag
//
LOCUS       DATLVW010000858         5171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_161312, whole genome shotgun sequence.
ACCESSION   DATLVW010000858 DATLVW010000000
VERSION     DATLVW010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161312"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VNN25_25150"
     CDS             complement(<1..828)
                     /locus_tag="VNN25_25150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH94885.1"
                     /translation="MNPTRAIVRGWWREALGAVIVLVSRILTMPRTPWENDEFLFAEA
                     VRSFDPSRYHPHPPGYPLFVILGKVFNAFVHDPWRALVIFGVIAAPIGFVALARAFRN
                     WIDDPDLAVCGVLLYYFSASMIVHGSLALSDGPAMMFVALALFAVSSPHDTEHERNAI
                     AIGIWTSAAIGTRPQLLIPIAPMLLIALLQMRTLRQRVACIVAFIILSLMWFLPLADA
                     AGGFNALRLYETKQMQYVAAHDASVSRGGMSTAMLVVRFIFHPWGWKYVTLPLFACVT
                     "
     gene            818..1444
                     /locus_tag="VNN25_25155"
     CDS             818..1444
                     /locus_tag="VNN25_25155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXH94886.1"
                     /translation="MGFIRALIVLHRVYGRRPLRDRIHTLVRFLTCPMLRVLDAVPRG
                     AALLDIGAGHGVFATLVRARDTRRVVGVEPDVRKVRPIAGVESVIGFDDVITGTFDAI
                     SIIDVLYKIPIADWDALLVRCRERLAFDGILIIKEQDPTARVKNAWNALQERIATALH
                     LTLGDSFTYEAPSVFLARLRRLGFADARSKRIDFGYPHPHVLYVAKKL"
     gene            complement(1518..2279)
                     /locus_tag="VNN25_25160"
     CDS             complement(1518..2279)
                     /locus_tag="VNN25_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXH94887.1"
                     /translation="MSSEPLRVIIIDDEPLARDSIRALLEGDAEVRVVGEGTGAEGAA
                     LIARTRPDLMFLDIQMPEVDGFALLEQVGAATVPAVIFVTAYDRYALKAFEVHALDYL
                     LKPFDDGRFASALAHAKERARGILRGEVDTRIAELLRERSEASRSRFLIPVRDKTIVV
                     DAADIDWIEASDYYVTIHSGTSAHLLRQTMDEIETQLDARLFFRVHRSTIVNIDRVRE
                     IHPLFRGDCALVLAGGQRLKLSRSRRKDFEARFRK"
     gene            complement(2276..3337)
                     /locus_tag="VNN25_25165"
     CDS             complement(2276..3337)
                     /locus_tag="VNN25_25165"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXH94888.1"
                     /translation="MSSRRVAWQFAALFWTAFGLVSGFQVWISMITHGHSVPLLIGYY
                     VLVWEAWLGATALIFWLAKRFPVVPPKRMSILVHALAACALGVVHSLYSVTLMILMRP
                     YDRMTAEVSQLHISEIIFYRLPLVWILYCLVLGSAIALEYYERYRERALQAAQLEASL
                     SDARLQALELQLQPHFLFNTLNAISALVRSRRNDEAVTMMAGLSELLRYTLDHSGNPQ
                     VALGEEISVLRRYLEIQRARFPDRMSFTIDASTEAERGLVPTLLLQPLAENAVKHGIE
                     VSASPGVITVRAARSGDRLTIEVFNSGILGTHSNGGIGLRNTLARLRHIHGNEGRFDL
                     TSVNGGVLAAVSVPWQERA"
     gene            3573..>5171
                     /locus_tag="VNN25_25170"
     CDS             3573..>5171
                     /locus_tag="VNN25_25170"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94889.1"
                     /translation="MSAVRTLRTFVFALCALIAPALSAQSIFTYAGGGTRDGLQAKSI
                     VLTAPYGLAADTKGNLYIAEESGSSVQKVNLATGVIERFAGNGGGSYSGDGGPAREAS
                     LKRPRGLTFDDTGNLFIADQDNGRVRRVDGKTGVITTFAGGTIDPNIVDAGDGGLATN
                     ALLAQPIGVFWRDGELYIAEDNYDANVVRKVDRNGIITTVAGKRGLQSFSGDDGPATS
                     AGLANPLASVVDADGNLYVADFTNGRLRRVDHVTHKITTVAGGGKEPDNLGDRPGLEA
                     NFCPAALAIDAAGLIYVADPCHRRVLRFTPSTGRIALFMGSGQYGGGDGSVATEAGVE
                     QPYALTLDAAGNLFVEDGSNGSIRRVDATTRIVSTVAGGGTFVGDGRVANAALLAGPR
                     GVAFDKQGNLLIADTEHSLVRRVDAVTNVISTFAGTVNVCCSGPDAPGTLATETTVGF
                     PVDIAVSSDGSVFFSDFQGSIWVVNTKGEIARFAGGNDTNGGNDSDDRLALTASIAPV
                     GIALDAANNVYLAERERNRVRKINA"
BASE COUNT         1001 a         1686 c         1631 g          853 t
ORIGIN      
        1 cgtgacgcag gcgaagagag ggagcgtcac gtacttccag ccccacggat gaaagatgaa
       61 gcggaccacc agcatcgccg tcgacatccc gccgcgcgac accgaggcat cgtgggcggc
      121 aacatactgc atctgcttcg tctcgtagag gcgcagagcg ttgaagccgc cggcggcatc
      181 ggcgagcggc aggaaccaca tcaacgacag aatgatgaat gccacgatgc atgccacgcg
      241 ctgccgtaac gtccgcatct gcaacaacgc gatgaggagc atcggcgcga tcgggatgag
      301 aagttgcgga cgcgtaccga tcgccgccga agtccagatg ccgatggcga tggcgttgcg
      361 ctcgtgctcc gtatcgtgag ggctgctgac ggcgaagaga gcgagcgcca cgaacatcat
      421 cgccgggccg tcggaaagcg cgagggatcc gtgaacgatc atcgacgccg agaagtaata
      481 gagcagcacg ccgcaaacgg cgaggtcggg atcgtcgatc cagttccgaa aggcgcgcgc
      541 caaggcgacg aacccgatcg gcgccgcgat gacgccgaag atcacgagcg cgcgccaggg
      601 atcgtgaacg aaggcgttga agaccttgcc gaggatgacg aatagcgggt agccgggcgg
      661 gtgcgggtgg taccgcgacg gatcaaaact cctcaccgcc tcggcgaaca gaaactcgtc
      721 gttttcccac ggcgtacgcg gcatggtgag gatgcgcgac accagcacga tcaccgcgcc
      781 gagcgcttcg cgccaccagc cccgtacaat cgctcgtgtg ggattcatcc gcgcgttaat
      841 cgtactgcac cgcgtttacg gccggcgccc gttgcgcgac cgcatccata cgctcgtgcg
      901 cttcctcacc tgcccgatgc tgcgcgtgct cgacgcggtg ccgcgtggcg cggcgctgct
      961 cgacatcggc gcaggccacg gcgtcttcgc aacgcttgtt cgtgcacgcg acacgcgacg
     1021 cgtcgtcggc gtcgagcccg acgtgaggaa agtgcggccg atcgccggcg tggagtccgt
     1081 catcggcttc gatgacgtca tcacaggcac gttcgacgcg atctcgatca tcgacgtcct
     1141 ctacaagatc ccgatcgccg attgggacgc gctgctcgtg cgctgccgcg agcgcctcgc
     1201 tttcgacggc attctcatca tcaaagagca agatccgact gctcgggtga agaacgcctg
     1261 gaacgcgtta caggaacgca tcgccacagc gctccatctc acgctcgggg actcattcac
     1321 gtacgaggct ccgtccgtat tcctcgcgcg tctcagacgc ctcgggttcg cggatgctcg
     1381 cagcaagcgg atcgacttcg gttatccgca tccgcatgtg ttgtacgtgg cgaaaaagct
     1441 ttaaccacaa agacacaaag gacacaaaga aagcctttgt gtcctttgtg tctttgtggt
     1501 gattgcttct gagaaattca ctttcggaaa cgagcctcga agtccttccg acggctccga
     1561 ctcaacttca gccgctggcc gccggcgagc accagcgcgc agtccccccg gaacagcgga
     1621 tggatctcgc gcacgcgatc gatgttcacg atcgtcgagc gatgcacgcg gaagaagagc
     1681 cgtgcatcga gttgcgtttc gatctcgtcc atcgtctggc gaaggagatg cgctgacgtg
     1741 ccgctgtgga tcgtgacgta gtagtcggaa gcctcgatcc agtcgatgtc ggcggcatcg
     1801 acgacgatgg tcttgtcgcg cacgggaatg agaaagcgcg accgcgacgc ctcgctccgc
     1861 tcccgcaaca actcggcgat acgcgtatcg acctcgccgc gcaggatccc gcgagcgcgc
     1921 tccttcgcat gtgcgagcgc gctggcgaaa cggccgtcgt cgaagggctt gagcagatag
     1981 tcgagcgcat gtacctcgaa tgccttgagc gcgtagcggt cgtaggcagt gacgaagatg
     2041 accgccggga ccgtcgcggc gccgacctgt tcgagcaacg cgaatccgtc cacctccggc
     2101 atctgaatat cgagaaacat caagtccggc cgcgtgcggg cgatgagcgc cgccccctcc
     2161 gcccccgttc cctcccccac cacccttacc tcggcatcac cttcgagtag cgcgcggatc
     2221 gagtcgcgcg ccagcggctc gtcatcgatg atgatgacgc gcaacggctc gctgctcatg
     2281 ccctctcctg ccacggcacc gacaccgctg cgagcacgcc gccgttcacg ctggtgagat
     2341 cgaatcgtcc ttcgtttccg tggatgtggc gaaggcgcgc cagcgtgttg cgcagaccga
     2401 tcccgccgtt gctgtgcgtg ccgaggatgc cactgttgaa tacctcgatc gtgaggcggt
     2461 caccgctgcg cgccgcgcgc accgtgatca cgcccggcga tgcgctgacc tcgattccgt
     2521 gcttcaccgc gttctccgcc agcggctgca acaacagcgt gggcaccaat ccccgctccg
     2581 cctcggtgga cgcatcgatg gtgaacgaca tgcgatcggg aaagcgggcg cgctggatct
     2641 cgaggtagcg gcggaggacg gagatctctt cgccgagggc gacttgcggg ttgccggagt
     2701 gatcgagcgt gtaacgcagc agctcgctca agcccgccat catcgtaacc gcctcgtcgt
     2761 tgcggcggct gcgcacgaga gcactgatcg cgttcagcgt gttgaacaga aagtgcggct
     2821 gcagttgcag ctcgagcgcc tgcaaacgag catcggagag tgacgcttcg agctgggcag
     2881 cctggagcgc gcgctcgcgg taccgctcgt agtactcgag cgcgatcgcc gagccgagga
     2941 cgaggcagta gaggatccac acgaggggca gccggtagaa aatgatctcg ctgatgtgca
     3001 actgcgagac ctcggcagtc atccgatcgt acggccgcat cagaatcatc aatgtgaccg
     3061 agtaaagact gtgaacgaca ccgagcgcgc acgccgccag cgcgtgcacg aggatcgaca
     3121 ttcgcttcgg aggcacgacg gggaatcgct tcgccagcca gaagatgagc gcggtcgccc
     3181 cgagccacgc ctcccagacg aggacgtagt atccgatcag cagcggcacc gagtggccgt
     3241 gcgtgatcat gctgatccag acctgaaatc cggagactaa tccgaacgca gtccagaaca
     3301 acgcagcgaa ttgccacgcc acgcggcgag agctcatgcc cgtcagtctg cccgagcctt
     3361 ggccaaaacg cgaaggaatg cagcgaacgg tgtcgcagag gctgtgaacc ggcggagaca
     3421 gcttcgatgg ctgggggtca agtcacgcga aggcgcgaag acgcgaagct gactaagaat
     3481 gtcttcgcgt cttcgcgcct tcgcgtgccc acttcagcac ccattccttc caactttcca
     3541 cgcgaggcga gtatcgtgat ggcctcacgc aaatgagcgc cgtccgtaca ctccgcacgt
     3601 tcgtcttcgc gctttgcgcg ctgattgcgc ccgccctctc cgcgcagtcc atcttcactt
     3661 acgcgggcgg cggcacgcgt gacggcttgc aggcgaagtc catcgtgttg actgcgcctt
     3721 acgggctggc cgctgacacg aaggggaatc tctacatcgc cgaggagagc gggagcagcg
     3781 tgcagaaggt gaatctcgca accggcgtga tcgagcggtt cgcgggcaac ggcggcggaa
     3841 gttacagcgg cgacggcgga ccggcgcgcg aggcatcgct caaacggccg cgcgggctca
     3901 ccttcgacga taccggcaac ctcttcatcg ccgatcagga caacggacgc gtgcggcgtg
     3961 tcgatggaaa gactggtgtc atcaccacct tcgcaggcgg cacgatcgat ccgaacatcg
     4021 ttgacgcggg cgatggggga ctggcaacga acgcgctcct cgcacagccg atcggcgtct
     4081 tctggcgcga cggcgagctg tacatcgccg aagacaacta cgacgccaac gtcgttcgca
     4141 aagtcgatcg caatgggatc attaccactg ttgccggcaa gcgcggactg caatcgttct
     4201 ccggcgacga cgggccagcc acctcggcgg gtctcgccaa tccgctggcg tcggtcgtcg
     4261 acgccgacgg caatctctac gtcgccgatt tcaccaacgg acgtctgcgg cgcgtcgatc
     4321 acgttacgca caagatcacc accgtcgccg gcggcggcaa ggagccggac aacctcggcg
     4381 atcgtcccgg cctcgaagcg aacttctgtc ccgccgcgct ggccatcgac gcagccggct
     4441 tgatctacgt cgccgatccc tgccaccgcc gcgtgctccg cttcacgccc tccaccggcc
     4501 ggatcgcgct gttcatgggt agcggccaat acggcggcgg cgacggcagt gtcgcgaccg
     4561 aggcaggcgt cgaacagcct tacgcgctga cgctcgacgc ggccggcaat ctcttcgtcg
     4621 aggatggatc gaacggaagc atccgccggg tcgacgccac gacgcgcatc gtgtccaccg
     4681 tcgcaggcgg aggcacgttc gtcggcgacg gacgcgttgc gaatgccgcg ctcctcgcag
     4741 gcccgcgcgg cgttgccttc gacaaacagg ggaatctgct gatcgccgat accgagcaca
     4801 gtctcgtgcg gcgcgtcgac gcggtgacga acgtcatctc gacgtttgcg ggaaccgtca
     4861 acgtctgctg cagcggccct gatgctcccg gtacgctggc cactgaaaca acggtcggct
     4921 tcccggtcga tatcgcggtg tcgtccgacg gcagtgtctt cttctccgat ttccagggct
     4981 cgatctgggt cgtcaacacc aaaggcgaga tcgcgcgatt cgcaggcggc aacgatacga
     5041 acggcggcaa cgacagcgac gaccgcctcg cgctcaccgc cagcatcgcg ccggtcggca
     5101 tcgccctcga cgcagcaaac aatgtttatc ttgccgagcg ggaacgaaac cgcgtgcgca
     5161 agatcaacgc g
//
LOCUS       DATLVW010000859         4638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_613703, whole genome shotgun sequence.
ACCESSION   DATLVW010000859 DATLVW010000000
VERSION     DATLVW010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613703"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VNN25_25175"
     CDS             <1..1114
                     /locus_tag="VNN25_25175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94890.1"
                     /translation="LWSTGATTQSITASASGNYSVTVTNANGCSATSSPTVVTANANP
                     TASITAGGPTTFCAGGSVTLTASSGASYLWSTGATTPSITVSAGGNYTVAVTNANGCS
                     ATSAPASVTVRPNPSATVSGGGAICAGSSATITATLTGTAPWNVTWSDNVTQPVSSGT
                     TATRSVSPSSSTTYTVTSVSDASGCSIAGSGSAAVTVKTPTATVSGNAILCPGVSATI
                     TAALTGTAPWSVKWSDNTTQTINAGTTATRTVNPSVTTTYTVTSVTDASGCPAVAGSG
                     SATITRNVAASITTQPANKSTTVNTSVTVTVVAAGSSPIFYQWFQSNGTIVTGATSSS
                     YTTSFPAKGNYQFYVEVWNSCNTTHVKSHNVTVTVN"
     gene            1172..2536
                     /locus_tag="VNN25_25180"
     CDS             1172..2536
                     /locus_tag="VNN25_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH94891.1"
                     /translation="MTKTLLDLARDHAGDFLGQLPDCHVGPRATRDELVAALKVPLTD
                     EGEDPAGVLDALVRGADRGLIACAGPRYFGFVIGGSLPVALAADWLTSTWDQNAGIYA
                     TSPAASVIEDIAREWLLELFDLPRSASVGFVTGGQMANFTALAAARHGVLRRVGWNVE
                     EDGLAGAPRVHLVVSAASHITIHSSMRMLGFGTRSLLKVDADDQGRMRADSLREVLAT
                     LDGPVIVCAQAGNVNSGAVDPLRALAAIAHERNAWLHVDGAFGLWGRATPSKRMLLDG
                     CEMADSWANDAHKWLNVPYDCGIVIVRDSASHRAAMTVSAEYLEQTSGAERDNFDWVP
                     EFSRRARGFAVYAAIRTLGRRGIAAMIDGCCERAVQFAELLARDPRVAILNDVVLNQV
                     LVRFGDSDELTRDVISRVQKEGTCWLAGTTWQGKAAMRISVSNWSTTAEDVEKSAAAV
                     LRCI"
     gene            complement(2591..3724)
                     /locus_tag="VNN25_25185"
     CDS             complement(2591..3724)
                     /locus_tag="VNN25_25185"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HXH94892.1"
                     /translation="MTSLRLELLGDFLIRTADGSLATIGAKKSQALLAYLAVKPGQHV
                     SREKLAGLLWSSTGPDQARQSLRQTLSTVRKELSQIAPKERTLIEDGDLLSLDASVTC
                     DAAEMEALIATGTEAGIAQALDLYRGDFLDGFYINEERFDQFVIGERDRLHRLALRAH
                     AQMVELQSRSGRLDEAIATAQRSLRLDILQEPMHRTLMRLYLQSGDLVEALQQYEHCA
                     RVLKRELSIEPDAETKALQREIVQLRAKRGAAASTDVDRTKTVLVVEDNALSRELTKA
                     VLKSAGYSVVLAKDGAEAMMLVGRDKVDLLLIDVDLPYIDGHSLLQAMHENGVNVPAV
                     FISGLPGEDVEIRAFEVGAADFIRKPVTNSVLLARVAKVMKES"
     gene            3816..>4638
                     /gene="mgtE"
                     /locus_tag="VNN25_25190"
     CDS             3816..>4638
                     /gene="mgtE"
                     /locus_tag="VNN25_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXH94893.1"
                     /translation="MTEMASVGDEMRELIRLQSWNQLRDRLTDLHPSDVADLIIALPR
                     EEEGFVFRVLPRDEAGEVFSHLPPDHQEGLIESLSNEQVRSVLSEMNPDDRIRLLEEM
                     PAVVTRRLLTALSPDDLKSARWLLGYPEDTAGRYMTPAYVTIEPSMTASQALDHIRAT
                     GSGKETVNVVYIVDREGRLVEDIRLSSLVLAEPSTKVTDIDDPPLVKMNATDDREEVL
                     KAFEKYDRLALPVTDAQGHMLGIITVDDVLDVAEQEATEDIQKLGGMEALDAPYLQ"
BASE COUNT          843 a         1504 c         1459 g          832 t
ORIGIN      
        1 tctctggtcg accggcgcga cgacacagtc gatcaccgcg agcgcgagcg gcaactacag
       61 cgtgacggtc accaacgcaa acggttgcag cgcgacgtca tcgccgaccg ttgtcactgc
      121 gaatgcaaat ccgacggcga gcatcaccgc cggaggaccg acgacgttct gcgcgggcgg
      181 cagcgtgacg ctgacggcat cgagtggcgc gtcctatctc tggtcgaccg gagcaacaac
      241 tccctccatc acggtcagtg ccggcggcaa ctacaccgta gccgtcacga acgccaatgg
      301 ctgcagtgcc acctcggcgc ctgcatccgt cacagtgcgt ccgaatccga gcgccacggt
      361 cagcggtggc ggcgcgatct gcgcgggcag ctcggccacg attaccgcaa cactgactgg
      421 taccgctccg tggaacgtga cgtggtcgga taacgtgacg cagccggtct cgagcggcac
      481 gacggccacc cgcagtgtca gcccgtcgtc gtcgacgacc tacacggtga catcggtgag
      541 cgatgcctcg ggatgctcga tcgccggcag cggcagcgcg gccgtcacgg tcaagacccc
      601 aaccgccacg gtgagcggca atgccatcct ctgtcccggc gtttcagcca ccatcaccgc
      661 agcacttacc ggcaccgcgc catggagcgt gaagtggtcg gataacacga cgcagacgat
      721 caacgccggc acgacggcca ctcgcaccgt gaacccatcc gtaacgacga cctacaccgt
      781 cacctcggtc accgacgcat cgggatgccc ggccgtcgcc ggcagcggca gcgcaacgat
      841 tacccgcaac gtcgcagcct ccatcaccac gcagccggcc aacaagtcga cgaccgtcaa
      901 cacgagcgtc acggtcacgg ttgtggccgc gggctcgtcg cccatcttct atcagtggtt
      961 ccaaagcaac ggaacgatcg tcaccggcgc cacgtccagc tcctacacca cctcgttccc
     1021 ggcgaaaggg aactaccagt tctacgtaga agtgtggaac agctgcaaca ccacgcacgt
     1081 aaagagtcat aacgttaccg tcaccgtgaa ttagcgtgtc cgaggtgcgg ttggcggttt
     1141 cgcagccgca cctcgtatta cgattcgcag cgtgactaaa actcttctcg atcttgcccg
     1201 cgaccatgcg ggagactttc tcggacaact tcccgactgc catgtcggcc cacgcgccac
     1261 gcgcgatgag ctcgttgccg cgctcaaggt gcctttgacg gatgagggcg aggatcctgc
     1321 cggcgtgctc gacgcgctcg tacgcggggc ggaccgcggt ctcatcgcct gcgccggccc
     1381 gcgctatttc ggtttcgtga tcggcgggag tctgccggtc gcgctcgccg cggactggct
     1441 gacctctacg tgggatcaga acgccggcat ctacgcgacg tcgcccgcag cgtcggtcat
     1501 cgaagatatc gcgcgcgagt ggctgctcga gctcttcgat cttccgcgct cggcgagcgt
     1561 cggcttcgtc accggcggac agatggcgaa cttcacggcg ctggcggcgg cacgtcacgg
     1621 cgtgcttcgc cgcgtcggtt ggaacgttga agaggatggt ctggctggtg cgccgcgcgt
     1681 tcatctcgtg gtcagcgccg cctcgcacat cacgattcat tcatcgatgc ggatgctcgg
     1741 attcggaaca cgatcgcttc tcaaggttga tgctgacgat caaggcagga tgcgcgccga
     1801 ttcacttcgc gaggttctgg cgacgctcga tggcccggtg atcgtttgtg cgcaggcggg
     1861 caacgtgaac agcggtgccg tcgatccgct gcgtgcgctc gcggcgatcg cgcatgagcg
     1921 gaacgcctgg cttcacgtcg acggcgcgtt cggcttgtgg ggacgggcca caccatcgaa
     1981 gcggatgctg ctcgatggat gcgagatggc cgattcgtgg gcgaacgacg cgcacaaatg
     2041 gctcaacgtt ccgtacgact gcggtatcgt catcgttcgc gattcggcat cgcatcgcgc
     2101 ggcgatgacg gtctctgcgg agtatctgga gcagaccagc ggcgcggagc gcgacaactt
     2161 cgactgggtc cccgagttct cccgccgcgc ccgtggcttc gctgtttacg ctgcgatccg
     2221 cacgctcggg cggcgcggaa tcgccgcgat gatcgatggc tgttgcgagc gcgcggtgca
     2281 gtttgcggag ttgctggccc gcgatccgcg cgtggccatc ctcaacgacg tcgttctaaa
     2341 ccaggtgctc gttcgtttcg gcgacagcga cgagctcacg cgcgacgtga tcagccgcgt
     2401 gcagaaggaa gggacgtgct ggctggccgg tacgacgtgg caggggaaag cggcaatgcg
     2461 gatctcggtc tcgaactggt cgacgacggc ggaggacgtg gagaagtcgg cggcggcagt
     2521 gctgcgctgc atttgagctg ccggcgcgtt cgttacgccg tcacgccacc tgcgcgatgc
     2581 ccacagggaa tcacgattcc ttcatcactt tcgccacgcg cgcaaggagc acgctgttcg
     2641 tcaccggctt tcggatgaaa tcagcagctc cgacctcgaa cgcgcggatc tcgacatctt
     2701 cgccgggaag cccggagatg aagacggccg gcacgttgac gccgttctca tgcatcgcct
     2761 gcagcagcga gtggccatcg atgtagggca ggtcgacatc gatgaggaga agatcgacct
     2821 tgtcccgtcc caccagcatc atcgcctcgg cgccatcctt cgccagcacg accgagtagc
     2881 cggcgctttt caggaccgcc ttcgtgagct cgcggctcaa cgcgttgtcc tcgacgacca
     2941 ggacggtctt tgtcctgtcg acgtcagtgc tcgcggccgc gccgcgcttt gcgcgcagtt
     3001 gcacgatctc tcgctgtagc gccttcgtct ccgcgtcggg ctcgatgctc agctcccgtt
     3061 tcagcaccct cgcgcaatgc tcatactgct gcagcgcctc cacgagatct ccgctctgca
     3121 ggtagagccg catcaacgtg cgatgcatcg gctcctgcag aatgtcgagg cgcagcgacc
     3181 gctgcgcggt ggcaatggct tcgtccaatc ttccgcttcg cgactgcagt tcgaccatct
     3241 gcgcgtgggc gcgaagtgcg agccggtgga gacggtcgcg ctctccgatg acgaactgat
     3301 cgaatcgctc ttcattgatg tagaagccat cgaggaagtc gccgcgataa agatcgaggg
     3361 cctgcgcgat tcctgcttcc gtgccggtgg cgatcaacgc ctccatctcc gcggcatcac
     3421 atgtaaccga cgcgtcgaga ctgaggagat cgccatcctc gatgagcgtt cgctccttcg
     3481 gggcgatctg cgacagctcc ttgcgtacgg tcgagagcgt ctgtcgaaga ctctgtcgcg
     3541 cctggtcggg tccggtgctc gaccagagca gaccggccag cttctcgcgg gagacatgct
     3601 gccccggttt cactgccaga tacgccagca atgcctgcga tttcttcgcc ccaatcgtgg
     3661 cgagtgagcc atcggcggtg cggatcaaaa aatcacccag cagctccaat cggagtgaag
     3721 tcatgttgtg agtggcaatt atctcagtaa gttggggaaa agtgtttcga ctttcatggc
     3781 cgcttcccgt actctccaaa aaaggaggtc gtgcgatgac ggagatggct tcggttggcg
     3841 atgagatgcg ggaactgatc cggctgcaga gctggaacca gctacgcgac cgcctgaccg
     3901 accttcaccc gtccgacgtc gccgatctga tcatcgccct gccccgggaa gaggagggct
     3961 ttgtcttccg cgtccttccg cgcgatgaag cgggcgaggt gttctcgcat ctgccgcccg
     4021 atcaccagga aggcttgatc gagtcgctca gcaacgagca ggtgcgcagc gttctgtccg
     4081 agatgaaccc ggacgaccgc atccggcttc tcgaggagat gccggcagtg gtaacccgcc
     4141 ggctgctgac ggcactttcg ccagacgatc tgaagagcgc gcgctggctc ctcggctatc
     4201 ccgaggatac ggccggccgc tacatgacgc cggcgtacgt cacgatcgag ccttcgatga
     4261 ccgcgagcca ggcgctcgat cacatccgcg ccaccggttc gggcaaggag accgtcaacg
     4321 tcgtctacat cgtcgacagg gaaggccgcc tggtggagga catccgcctc agttctctcg
     4381 tgctggccga gccgtcgacg aaagtcacgg acatcgatga cccgccacta gtgaagatga
     4441 acgccacgga cgaccgcgag gaagttctga aagcgttcga gaagtacgac cggctggcgc
     4501 tgcccgtgac ggacgcgcaa gggcacatgc tcggcatcat caccgtcgac gacgtgctcg
     4561 atgtcgcaga gcaggaagcg accgaggaca tccagaaact gggcggcatg gaagcgctcg
     4621 atgcgccgta cctgcagg
//
LOCUS       DATLVW010000860         7511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_245114, whole genome shotgun sequence.
ACCESSION   DATLVW010000860 DATLVW010000000
VERSION     DATLVW010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245114"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VNN25_25195"
     CDS             <1..780
                     /locus_tag="VNN25_25195"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXH94894.1"
                     /translation="PPLRSGVVDASAVIDALRPETRLVAMMLANNETGVIQPVAEVAR
                     VCRERSIHFHCDAVQGAGKIAIDVSELGIDTLALSAHKLHAPKGIGALYVRRGLALDR
                     HILGGAQERRRRAGTENVPLAVAFGVAADLARKPSEVGVLHDALESSLRESGFSIVIN
                     GVDAPRIPNTSSVTFRGADGEGIVIGLDLNGVAVSTGSACSSGRVEPSHVLLAMGLTP
                     DEARSTVRFSLSRFTTREEIEQTVAILREVVPRCVRVARET"
     gene            916..2061
                     /locus_tag="VNN25_25200"
     CDS             916..2061
                     /locus_tag="VNN25_25200"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXH94895.1"
                     /translation="MPENQILTGDLSRIQLPDVLSFLAMIRGSGKLLLRKPPMERSIH
                     WKDGEIVFASSNSPEHSLGMFLLRNGKITQTQYDESKRRVTAQMRHGKLLVQMGAISP
                     KDLWWGVKNQALEIIYSLFSWKEGVFVFGDTQDELLNERIVLSINTATVIMEGIRRLD
                     ETAMIREKITSLDMVFVKEQGAVPDFASLELSDHEVELYNAIDGKQTIRALISRSEMT
                     EFEVTRILFQLLSARLIEVSSEEKSFRPVFLDVEDSPELLKVISTYNDMFGRLYEALL
                     GAVGEETARDIVMTAMQNAESDELWSGVFFDQYGRFDENMLIANISELPFERRKAVLD
                     EGLNTLLSVQLFEVSQHLDSAGKVDVFRFISDQKASLESAFTARSLS"
     gene            2125..2925
                     /gene="lgt"
                     /locus_tag="VNN25_25205"
     CDS             2125..2925
                     /gene="lgt"
                     /locus_tag="VNN25_25205"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXH94896.1"
                     /translation="MYPTLIKIGSFEITTFGLMMFAAFIVGGWVLTRQFRRYGLSDDA
                     ASSMVMAAALGGIAGAKIYYAILFHDWHLLLDRAGLVWYGGLIGGFLGCAGYLLAKKI
                     DFLTAADAAAPGLAIGYALGRIGCFLVGDDYGRPTNSWIGIAFPKGSPPTTAEALRQF
                     GVRVDPSIPPDEILRVHPTQLYESAAAFVMFAILMAVSRRSYSRGRIFGLFLILMGIE
                     RFLVEIVRAKDDRFLGPFTIAQLISVIILLIGATLVAKSREGTIQEAR"
     gene            complement(3528..4700)
                     /gene="aroC"
                     /locus_tag="VNN25_25210"
     CDS             complement(3528..4700)
                     /gene="aroC"
                     /locus_tag="VNN25_25210"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXH94897.1"
                     /translation="MSRFTFLSAGESHGPQLTVIVTGVPAGVRLDRNRINRDLARRQH
                     GYGRGGRMKIEDDEIEIVGGVRGGETLGSPIAIVIRNADFKNWEAAMGVWEVDADEAH
                     KRRVHAPRPGHVDLIGGMKYDRRDLRDVLERASARETASRVAAGAIAKELLRAFGIEL
                     RSGVVSIGAAGDPDRAMTWDDLGNVDDDSPMRAVDRDTEAAMVAEVDQAREAGETLGG
                     TIVAAAHNVPIGLGSYVQWNEKLDGRIAQAILSIHAVKAVAIGDGVAAAQRVGSEVHD
                     PIYFDEERRYHRTSNRAGGLEGGVTNGQDVVVRAYMKPISTLRRGLPSVDIETHEPHR
                     SQWERSDVTAVPACGVVCEAMLAIALADAMREKFGGDSLGEMRANYEGYVARIQEY"
     gene            4794..5924
                     /locus_tag="VNN25_25215"
     CDS             4794..5924
                     /locus_tag="VNN25_25215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXH94898.1"
                     /translation="MDKRALRELFPVTKNVVYFNHAAVGPLSTRSYDAMERFARDQRD
                     FGALHWRDWYAEYVHLRNAAARLIGAEPGEIAILKNTSEGLSFVAEGFRWERGDNVVT
                     TDLEFPSNSTPWRKLDRRGVETRVVRSHDGAFTVDDVEQCVDGRTRIVSVSSVAFHNG
                     FAADLDAIGAMCARRGVLFCVDAIQSVGVLPMDVRRSNIAFLAADGHKWMCGSEGAAI
                     FFVAAEHRDKLEVLENGWTNIERKGKFIDCPLDLLPDARRFEAGSLNTSGIYGLRAAI
                     DLLLEIGINDVAEESTRIAALLAERLDAIGWQVASPRPIRSAIVGALPPSSEPSLLKW
                     HRLLEENGIVCAPREGMLRFSPHFYNDEDEVERVIAVLKTTI"
     gene            6102..7454
                     /gene="rmuC"
                     /locus_tag="VNN25_25220"
     CDS             6102..7454
                     /gene="rmuC"
                     /locus_tag="VNN25_25220"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXH94899.1"
                     /translation="MTILLSVAALIIGAVAGWVVASARKSGDVQRAEAERDLARAERE
                     RSEAAVIEARRFAASESHARAVAETQASFLGDARKQMEEAFAATAQKALASISSSFLD
                     LNKAHMDGSKGELAASLDTKKAEIDTMLQPLRDMLGRYAGDLQKSEHSRNEAYGGLQE
                     QIRALLSAQESAQREASRLANALASPTVRGSWGENSLRRCIELAGMSELCDFDVQETF
                     LTDEGRRLRPDVVVHLPNKRIIAIDAKVPLSDYSVAANATEETRKLEALTAHARTLRR
                     HIDALSRKDYQLNIGDSLDFTVMFLPGEHFLSAALVTDPMLFEYAVDKKIYLASPTVL
                     LPLLRAVAAGWKAERTEENAKKMHDAGVELFNRFVKVMDLITDIGSALAKTVEQYNKA
                     IRSIDTRLWPKGQELQRMAGSGTELEALSQVDTLPLESSKLRLTMQGDEPAEVLPFRE
                     "
BASE COUNT         1369 a         2418 c         2324 g         1400 t
ORIGIN      
        1 ccgccgttgc gcagcggcgt cgttgatgcg agtgcggtca tcgatgcgct gcgcccggag
       61 acgcgtctcg tggcgatgat gctggccaat aacgaaaccg gcgtcatcca gccggtcgcc
      121 gaagttgcgc gcgtttgccg cgagcgatcg attcattttc attgtgatgc cgtgcagggc
      181 gcaggcaaga tcgcgatcga tgtgagcgag ctcggcatcg acacgctcgc gctctccgcg
      241 cacaagctgc atgcgccgaa aggcatcggc gcgctctacg ttcgccgcgg tctcgcgctc
      301 gaccgccaca tcctcggcgg cgcgcaggag cgccggcgac gcgcaggaac ggagaacgtt
      361 ccgctggccg ttgccttcgg tgtcgcggct gacctcgccc gcaagccgtc agaggttgga
      421 gtccttcacg acgcgctgga gagctcgcta cgcgaatccg gcttctcgat cgtcatcaac
      481 ggcgtcgacg cgccccgcat cccgaacacc agtagcgtga cgttccgcgg cgccgacggc
      541 gaaggaatcg tcatcggcct cgacctcaac ggcgtcgcgg tatcgaccgg ctccgcctgc
      601 tcctccggcc gcgtcgagcc aagccacgtc ctcctggcca tgggcctcac ccccgacgaa
      661 gcccgaagca ccgtccgctt ttcgctgagc cgcttcacga cgcgggaaga gatcgagcag
      721 acggtggcga ttcttcggga ggtggtgccg aggtgcgtgc gagttgcgag ggaaacgtag
      781 gtcggactct ctagtccgac ttggaggtgc cgtcggactg gagagtccga cctacgtttc
      841 cctcgggcgt ccgacccccg caccgaataa cccttgcccg agcccggttt gtctgagaca
      901 gttaactcgg tcctgatgcc ggaaaaccaa attctcacgg gcgatctctc gcgcattcag
      961 ttgccggacg tgctcagctt cctcgccatg atccgcggat cgggaaagct gttgctgcgc
     1021 aagcctccga tggagcgttc gatccactgg aaagacggcg agatcgtctt cgcctcctcc
     1081 aactctcccg agcactcgct cggcatgttc ctgctgcgca acggcaagat cacgcagacg
     1141 cagtacgacg agtcgaagcg gcgcgtcacg gcccagatgc gtcacggcaa actgctcgtg
     1201 cagatgggcg ccatctcgcc gaaggacctc tggtggggcg tcaagaatca ggccttggag
     1261 atcatctact cgctcttcag ttggaaagaa ggtgtcttcg ttttcggcga cacgcaggac
     1321 gagctgttga acgagcggat cgtcctctcg atcaacacgg ccaccgtcat catggaaggc
     1381 atccgccgtc tcgatgagac cgcgatgatc cgcgagaaga tcaccagtct ggatatggtc
     1441 ttcgtcaaag agcagggcgc agttcccgat ttcgcgtcgc tggagctgag cgatcacgaa
     1501 gtcgagctct acaacgccat cgacggaaag cagaccatcc gcgcactgat cagccgctcg
     1561 gagatgaccg agttcgaagt cacgcgcatc ctcttccagc tcctttcggc gcgcctgatc
     1621 gaagtgtcgt ccgaggagaa gtcgttccgt ccggtcttcc tcgatgtcga agactcgccg
     1681 gagttgctga aggtcatctc cacctacaac gacatgttcg gccgtctgta cgaagcgctc
     1741 ctgggcgcgg taggcgagga gaccgcccgc gacatcgtga tgacggcgat gcagaacgcg
     1801 gagagcgacg agctctggag cggcgtcttc ttcgatcaat acggccgctt cgacgagaac
     1861 atgctcatcg ccaacatctc cgagcttcct ttcgagcgcc gcaaggccgt gctcgacgaa
     1921 ggcctgaaca cgctcctctc cgtccagctc ttcgaagtgt cgcaacacct cgactccgcc
     1981 ggaaaagtcg acgtcttccg cttcatcagc gatcagaagg cgtcgctgga gtcagcgttc
     2041 accgcgcgaa gcctctcctg acgtcgtcct gggtcttgtt gctccgacac ccgacacgcg
     2101 acacccgcga tagactccac tcacatgtat ccgactctca tcaaaatagg ctccttcgag
     2161 atcacgacct tcggtctgat gatgttcgcc gcgttcatcg tcggcgggtg ggtgctgacg
     2221 cgccagttcc gacgctacgg actgagcgat gacgcagcct cctccatggt catggccgcc
     2281 gccctcggcg gaatcgccgg cgccaagatc tactacgcga tcctctttca cgactggcac
     2341 ctcctgctcg accgcgccgg cctggtctgg tacggcggcc tgatcggcgg cttcctcgga
     2401 tgcgccggtt acctcttagc gaaaaagatc gacttcctga ccgccgccga tgccgccgcc
     2461 cccggcctcg ccatcggcta cgccctcggc cgcatcggct gcttcctggt cggcgacgac
     2521 tacggccgcc ccaccaattc ctggatcggc atcgccttcc ccaaaggctc gcctcccacg
     2581 accgccgagg cactgcgcca gttcggcgtc cgcgtcgacc cgtcgatccc acccgacgag
     2641 atcctgcgcg tccaccccac gcagctctac gagtccgctg ccgccttcgt tatgttcgcg
     2701 atcctgatgg ccgtctcccg gcgttcgtac tcgagaggcc gcatcttcgg cctcttcctg
     2761 atcctcatgg gtatcgaacg cttcctcgtt gagattgtgc gtgcgaagga tgaccggttc
     2821 ctggggccgt tcacgattgc gcaactcatc agcgtcatca ttctcttgat tggagccacg
     2881 ctcgtggcga agagtcgcga ggggacgatt caggaagccc gataaccgct tatcgagtga
     2941 taggaggcct gacccgcaaa gcggatcgca gtgatcctcg attggattcg cagcgaaatg
     3001 tgatgtgtca tcctgagcct gccggaggcg cccgcgctgc tcaccgctct cgcgcggtgt
     3061 tgcattggcg ccgcaggacg gcgaaggatc tcaaaatacg aggcatctgg catgtgcggc
     3121 gtaatttgag atccttcgtc gtcctccgcc gccatggctt ttgccgcgaa cgcacgagtg
     3181 cgaatggcgg ctccggcagc ctcaggatga caggttggat aacacgtcaa ttgagggcct
     3241 tttcattggt gtgctagagg tacttgatcg tggggagacg acgatggccg aatttgtcac
     3301 ctacacctgt ccgaaatgta ccttttacgc agagtgcact aactgcgatg cttgcgatgg
     3361 gatcgtggtc tggacgaccc atggtccgga gtgcactggc tgtggaactg taattcagtg
     3421 gcacacctgt cgggtgtgtg gcgagcgctg ggatatttga agcaatctgc gtctggtgag
     3481 tcaacgaccg gtggccttcg ttaccgcatc aaacggagag tgcatgatta gtactcttgg
     3541 atacgcgcca catacccctc gtaattcgcc ctcatctccc ccaacgaatc ccctccaaac
     3601 ttttcgcgca tcgcatcggc tagcgcgatt gccagcatcg cttcgcagac gacgccacag
     3661 gcggggacgg cggtgacgtc gcttcgttcc cattgggagc ggtggggttc gtgcgtttcg
     3721 atgtcgacgg aggggaggcc gcgacggagg gtggagatcg gcttcatgta ggcgcgcacg
     3781 acgacgtcct ggccgttggt gacgccgcct tcgaggccgc cggcgcggtt cgaggtgcgg
     3841 tggtagcggc gttcctcatc gaagtagatc ggatcgtgaa cctcggaacc aacgcgttgc
     3901 gcggcagcga caccgtcacc gatggcaacc gccttgacgg cgtggatgga gaggatggcc
     3961 tgcgcgatgc ggccgtcgag tttctcgttc cactgcacgt aggagccgag gccgatgggg
     4021 acgttgtggg cggcggcaac gatcgtgccg ccgagggtct cgcctgcctc gcgtgcttga
     4081 tcgacctcgg cgaccatcgc ggcttcggta tcgcgatcga cggcgcgcat cggcgagtcg
     4141 tcgtcgacgt tgccgaggtc gtcccaggtc atggcgcggt caggatcgcc ggcggcaccg
     4201 atggagacga ctccggaacg aagctcgatg ccgaacgctc gcagcaattc cttggcgatg
     4261 gcgccggcag cgacgcgcga agcggtctca cgtgcggaag cgcgctcgag gacgtcgcgc
     4321 agatcgcggc ggtcgtactt catgccaccg atcaggtcga catgaccagg ccgcggcgcg
     4381 tggacgcggc gtttgtgggc ttcgtcggca tcgacctccc acacgcccat cgccgcttcc
     4441 cagttcttga agtcagcatt gcggatgacg atcgcaatcg gcgagccgag cgtttcgccg
     4501 ccgcgaacgc cgccgacgat ctcgatctca tcatcctcga tcttcatccg tccgccgcga
     4561 ccgtaaccgt gctgacggcg ggcaagatcg cggttgatgc gattgcggtc gaggcgaact
     4621 ccggccggaa cgccggtaac gatgacggtc agttgcggcc catgcgactc gccggcggag
     4681 aggaatgtga agcgcgacat gcgatccgca gtttagttca agccgatctg cggccatctg
     4741 cgccatctgc ggacaggttt ttgagacgca gatgacggcg atacacttcc ctcgtggaca
     4801 aacgcgcgct ccgcgagctc ttcccggtca cgaagaacgt cgtctatttc aaccatgcgg
     4861 cggtcggtcc gctctcgact cggtcctacg acgcgatgga gcgattcgcg cgcgaccagc
     4921 gtgacttcgg cgcgctgcac tggcgcgatt ggtacgccga atatgttcac cttcgcaacg
     4981 ccgccgcgcg gctgatcggc gcggaaccgg gcgagatcgc catcctcaag aacacgtcgg
     5041 aaggtttgtc gttcgtcgcc gagggctttc gatgggagag gggagacaac gtcgtcacca
     5101 ccgacctcga attcccctcc aactccactc cttggagaaa acttgacaga cgcggtgtgg
     5161 aaacgcgcgt ggtgcggagt cacgatggcg cgttcacggt cgatgacgtc gagcaatgcg
     5221 tcgacggacg aacgcgcatt gtcagcgttt cgtcggtcgc gtttcacaac ggcttcgccg
     5281 cggacctcga cgccatcggc gcgatgtgcg cgcggcgcgg cgtgctcttc tgcgtcgacg
     5341 ccatccaatc ggtcggcgtc cttccgatgg acgtgcgccg ctcgaacatc gccttcctcg
     5401 ccgccgacgg ccacaagtgg atgtgcggtt ccgagggggc ggcgatcttc ttcgtggcgg
     5461 cggaacatcg cgacaagctc gaagtgctcg agaacggatg gacgaacatc gagcgcaaag
     5521 gaaagttcat cgactgtccg ctcgatctcc tgccggacgc gcgccgcttc gaggccggat
     5581 cgctcaacac gagcggcatc tacggtttgc gcgcggcgat cgacctgttg ctggagatcg
     5641 gcatcaacga tgtcgccgaa gaaagcacgc gcatcgccgc gctgctcgcc gagcgtctcg
     5701 acgccatcgg ctggcaggtt gcatcgccgc gcccgatccg ctccgcaatc gtcggcgcgt
     5761 tgccgccatc gtccgaaccg tcgctcctca agtggcaccg gctgctcgaa gagaatggca
     5821 tcgtctgcgc gccacgagaa gggatgctgc gcttctcgcc tcatttctat aacgatgagg
     5881 atgaggtgga gcgcgtgatc gcggtcttga aaacaacaat ttgaaccaca gagacacaga
     5941 ggtcacaaag ttgtaatcga accctttgtg acctctgtgt ctctgtggta atgcctttaa
     6001 agactctacg tatcggagag ttgccgaacc cgcctcgata ggaatacccc aacccgcttg
     6061 accctctccc gcggagacct catcatcttt ccatgtccct catgacgatt ctcctcagcg
     6121 tggccgcact catcatcgga gcggtcgcag ggtgggtggt ggcgagcgcg aggaagagcg
     6181 gcgacgttca gcgcgccgag gccgagcgcg atctcgcgcg ggcggagcgc gagcgcagcg
     6241 aagccgccgt catcgaagcg cgtcgcttcg ccgcgtccga gtcccacgcc cgcgctgtcg
     6301 ccgagaccca ggccagcttc ctcggcgatg ctcgcaaaca gatggaagag gccttcgctg
     6361 caaccgcgca gaaggcgctc gcatcgatca gctcctcctt cctcgatctg aacaaagcgc
     6421 acatggacgg ctcgaaaggc gaactggccg cgtcgctcga cacgaagaaa gccgagatcg
     6481 acacgatgct gcagccgttg cgcgacatgc ttggccgcta cgccggcgat ctgcagaaaa
     6541 gcgagcactc ccgcaacgag gcctacggcg gattgcagga acagatccgc gctctcctca
     6601 gcgcccagga gagtgcgcaa cgcgaggcct cgcgtctcgc caatgcgctc gcgtcgccga
     6661 cggttcgcgg ctcgtgggga gagaactccc tgcgccgctg catcgagctg gcaggcatga
     6721 gcgagttgtg cgacttcgac gttcaggaga cgttcctgac cgacgaggga cgccgcctcc
     6781 gtcccgatgt cgtcgtccac ctaccaaaca agcggatcat cgccatcgac gcgaaagtgc
     6841 cgctcagcga ttacagcgtc gccgccaatg cgaccgagga gacgcgcaag ctggaagcgc
     6901 tcacggcgca cgcgcgcacg ttgcgccggc acattgacgc cctcagccgc aaggactacc
     6961 aactcaacat cggtgacagc ctcgacttca ccgtgatgtt cctgccgggc gagcatttcc
     7021 tctccgctgc gctggtcacc gatccgatgc tcttcgagta cgcggtcgac aagaagattt
     7081 acctcgcatc gccaacggta cttctgccgc tcctgcgcgc cgtggccgca ggctggaagg
     7141 cggagcgcac cgaggagaac gcgaagaaga tgcacgacgc cggcgtcgag ctcttcaacc
     7201 gcttcgtaaa agtgatggac ctgatcaccg acatcggctc cgcgctggcg aaaaccgtcg
     7261 agcagtacaa caaagcgatc cgctcgatcg acacccgcct ctggccgaag ggacaggaac
     7321 tgcagcgaat ggccggcagc ggcacggagt tagaagcgct gtcgcaagtc gacacgctgc
     7381 ccctcgagtc gtcgaaactg cgcctgacga tgcaggggga tgagccggcg gaggtgttgc
     7441 cgttcaggga gtgaatgatt gcggcgccct ggcaggcgcc gcgttgtcgg ttgtcggttg
     7501 gttgcgcttc g
//
LOCUS       DATLVW010000861         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_325020, whole genome shotgun sequence.
ACCESSION   DATLVW010000861 DATLVW010000000
VERSION     DATLVW010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325020"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VNN25_25225"
     CDS             <1..1416
                     /locus_tag="VNN25_25225"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH94900.1"
                     /translation="DEGEVDAARAAATQYIQVLQEKGDTAAIEDVGDEFAGRGYPLES
                     AVPKPAVRAPAPAPPVQAPPPPPPPEPAPVEEFADEMSFDIESAVADFELTAPSAPEP
                     AEPEPSFSFDSPDSSLSFEEPMSLLEPEPEPEPELEFTSPLSFESPIGEGFSFESPAE
                     EPGSEQPVFDVEVPSFSSAPSAEELRELDFYIEQELFDEAREKMDALSSRFPDASSIS
                     ERRARLAAAARPAQSEVRDRGAIAAPPVLSRDEIESELLSALPDDDDDFFAPPVQPEA
                     RVAVPPPSPPPPPVAAAAEENLFADEDDFFDLAAELESELEEEENVSLSEEEQSLEEI
                     FKEFKKGVEQQLDSEDYDTHYNLGIAYKEMGLIDEAIGEFQLASKDPKRSVECSSMLG
                     LCFLEKGMPQLAIKWYRKGLEMTEITADEHVGLLYDLGSAYVEVGDTDSAQKAFLEVY
                     GLNPNYRDIVTRIKQLEDARK"
     gene            1413..1907
                     /locus_tag="VNN25_25230"
     CDS             1413..1907
                     /locus_tag="VNN25_25230"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXH94901.1"
                     /translation="MNYKFESLLRFVNLTTSGLLAGSLGFGEQALVPGWQHELPNHED
                     RRARAIAALTDATSYFNAIGPVALGTAVTLAVASRGVKPLKRVLDAASAISLAGVLAA
                     TIMVTVPINKELDQQAPTDYPSDRSLSLAKNWSRAHAVRTTLGVGAFLCAVASNLARP
                     RQAK"
     gene            complement(1895..2800)
                     /gene="thiL"
                     /locus_tag="VNN25_25235"
     CDS             complement(1895..2800)
                     /gene="thiL"
                     /locus_tag="VNN25_25235"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXH94902.1"
                     /translation="MSERDVIRRIRKLFPRAGDDAAVVGTQVIANDVLVENVDFTRDV
                     PLRLIARKSLTINLSDIAAMGGRPHYAIVAIGSPDEALAATLIEELAVAAREQDVEII
                     GGDLSRAAEIFISITIIGEAAQPILRSGARRGDRIYLSRPIGGSAAGLTLLQRKTVGA
                     SYAEREFIESAIRRHLEPEAEVALGLKLVGIATSCIDVSDGLSTDLHHLCDASSVGAA
                     IERERIPVFPDLQRSGPQLGINVRDAVLHGGEEYALLFTSSMRESELSSRVGRPVYAI
                     GRIVEERGVRLDGEPLAPGGFDHFA"
     gene            complement(2797..3579)
                     /gene="truA"
                     /locus_tag="VNN25_25240"
     CDS             complement(2797..3579)
                     /gene="truA"
                     /locus_tag="VNN25_25240"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXH94903.1"
                     /translation="MPTYKLTIEYEGTRYSGWQAQGNTPKTVQGHLQRAAEQVLGDAD
                     IGGAGRTDSGVHAAAQVAHLRTHKGVDPPLLSRKINDLLPHDISIVAASRASDRFHAR
                     HDAVSRVYLYQIATRRTAFAKPFVWWIKDRLDVDAMTRAAKLLEGRHDFSAFSDKRMK
                     EEDSRIVVVTRCDVAAAGDLILFRIEASHFLWKMVRKLVAFLVEIGRGNAKPEDLAAH
                     LKKNAANVEPTAPPSGLFLEAVIYRGETFDRPLEPIVPVVRR"
     gene            3578..3895
                     /locus_tag="VNN25_25245"
     CDS             3578..3895
                     /locus_tag="VNN25_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXH94904.1"
                     /translation="MRPASYQVIGDLLAVLWDDGHESYYPLEALRRACPCAACSGEPD
                     LFGRMSSGPPARYTPASFQLDSVIPVGNYALQPNWADGHTYGMWTHESLRAFCPCGSC
                     GKK"
     gene            4032..4277
                     /locus_tag="VNN25_25250"
     CDS             4032..4277
                     /locus_tag="VNN25_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015147203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94905.1"
                     /translation="MKKEAETSHDATNEVDDLLPEYDFSGGARGKYYRPGKTTIRMTI
                     DEDVARYFYSAELLNAALRQLIVEGRAPEPRDEASGS"
BASE COUNT          807 a         1357 c         1368 g          783 t
ORIGIN      
        1 gatgaaggcg aggtcgacgc ggcgcgcgca gcagcgactc agtacattca ggttcttcag
       61 gagaaaggtg acaccgccgc gatcgaagac gtcggtgacg aattcgccgg gcgcggatac
      121 ccgctcgaat ctgcggtgcc caagcccgct gtccgagcgc ccgcgcctgc gccgcccgtt
      181 caagcgccgc cgcccccgcc tccacccgaa cccgcgccgg tggaggagtt cgcggatgag
      241 atgagcttcg acatcgagtc ggccgtggcg gatttcgaac tgactgcccc gagtgcgccg
      301 gagcctgctg agccagagcc ttcgttttcg ttcgattcgc cggactcatc tctgagcttc
      361 gaagaaccaa tgtcgttgct cgagccggaa ccggagcccg aacccgaact ggaattcacc
      421 agccctctct cgttcgagtc tcccatcggt gaaggctttt cgttcgaatc gccggccgag
      481 gagccgggtt cggagcagcc ggttttcgac gtcgaggttc catcgttcag cagcgctccg
      541 tctgccgagg agttgcgcga gctcgacttc tacatcgagc aggagctgtt cgatgaagcg
      601 cgcgagaaga tggacgcgct ctcctcacgt tttccggacg cgagcagcat cagcgaacgt
      661 cgtgcgcggc tggccgctgc cgccaggcct gcgcaatcgg aagttcgcga tcgcggggcc
      721 atcgctgcgc cgccggtcct ttcgcgcgac gagatcgaga gcgagctact ttcggcgctg
      781 cccgacgatg acgacgactt tttcgcgccg ccggttcagc ccgaggcgcg tgtcgcggtc
      841 ccgccgccat cgccaccgcc gccgcctgtt gccgctgccg ccgaggagaa tctgttcgcc
      901 gacgaagatg atttcttcga tctcgcggcc gagctggaat cagaattgga agaggaagag
      961 aacgtctcgc tcagcgaaga ggaacagtcg ctcgaggaga ttttcaagga gttcaaaaag
     1021 ggcgtcgagc agcagctcga ctccgaggac tacgacactc actacaacct cggcatcgca
     1081 tacaaagaaa tgggactcat cgacgaggct attggtgagt ttcaactggc gtcgaaggat
     1141 cccaaacgct cagtcgagtg ttcgtcgatg ctcggtctct gctttctcga gaagggcatg
     1201 ccgcaattgg cgatcaagtg gtatcgcaag ggtttagaga tgaccgagat caccgccgac
     1261 gagcatgtcg ggctactcta cgatctcggt tccgcctacg tggaagtcgg cgatactgac
     1321 agcgcgcaga aggcatttct ggaagtctac ggtttgaatc cgaactaccg cgacatcgtg
     1381 acgcgcatca aacaactcga agacgcccgc aaatgaacta caaattcgaa tcgctgcttc
     1441 gcttcgtgaa tctcacaacc tccgggctgc tggccggctc gctcggattc ggcgagcagg
     1501 cgctggttcc gggatggcaa cacgagttgc cgaaccacga ggaccggcgt gcgcgggcga
     1561 ttgctgcgct gaccgacgcg acgagctatt tcaatgcgat cggcccggtt gcgcttggta
     1621 ccgcggtgac gctcgccgtc gcctcgcggg gagtgaagcc gctcaaacga gttctcgacg
     1681 ccgcgtcagc gatcagcttg gccggcgttc tggcggcgac gatcatggtg acggttccga
     1741 tcaacaaaga gctcgatcag caggcgccga ccgactatcc gagcgaccga tcgttgtcac
     1801 ttgcgaagaa ctggtcgcgg gcgcacgcgg tgcgcacaac cctcggtgtg ggcgcgtttc
     1861 tttgcgctgt ggcttcgaac ctggcgcggc cacgtcaggc gaagtgatcg aatccgccgg
     1921 gcgcgagcgg ctcaccatcc agccgaaccc cgcgttcttc gacgatgcgg ccgatcgcgt
     1981 agacgggacg tccgacgcgc gagctcaatt cggattcccg catcgatgat gtgaagagga
     2041 gcgcgtactc ttcgccaccg tggagcacgg cgtcacggac attgatgcca agctggggtc
     2101 cggagcgctg cagatcgggg aagacaggaa tgcgctcgcg ctcgattgcc gcaccgacgc
     2161 tcgaggcatc gcagaggtgg tgaagatcgg tcgacaggcc gtcggagacg tcgatgcagc
     2221 tcgtagcgat gcctacgagt ttcaggccga gcgcgacttc cgcttcgggc tcgagatggc
     2281 ggcggatcgc cgactcgatg aattcgcgct ccgcatagct cgctccgacc gtcttgcgtt
     2341 gcaagagagt gagtccggca gccgagccgc cgatcggacg gctcaggtag attcggtcgc
     2401 cccgccgcgc accgctacgc agaatcggtt gcgccgcttc gccgatgatc gtgatcgata
     2461 tgaaaatctc ggcggcccgc gagaggtcgc cgccgatgat ctcgacatcc tgctcgcgtg
     2521 ccgccaccgc cagctcttcg atgagcgttg ccgcaagcgc ttcgtctggc gagccgatag
     2581 cgacaattgc gtaatgcgga cggccgccca tcgccgcgat gtcggagagg ttaatggtca
     2641 ggctcttgcg cgcaatgagg cgtagcggca cgtctcgggt gaagtcgacg ttctcgacga
     2701 gcacgtcgtt cgcgataacc tgcgtgccga cgaccgccgc atcgtcgccc gcccgcggaa
     2761 agagcttgcg aatgcgccgg atgacgtcgc gctcgctcaa cgccgtacca ccggcacgat
     2821 cggttcgaga gggcggtcga acgtttcgcc gcgatagatc accgcttcga gaaacaatcc
     2881 cgaaggtggc gcagtcggtt cgacgttcgc cgcgttcttc ttgaggtggg cggcgagatc
     2941 ttccggcttc gcattcccgc gcccgatctc gaccaggaac gcgaccagct tccgcaccat
     3001 cttccagagg aagtgcgaag cctcgatgcg gaagagaatg agatcgccgg cagcggcgac
     3061 gtcgcaacgc gtgacgacga cgatgcgcga gtcttcctcc ttcatccgct tgtcgctgaa
     3121 tgcgctgaaa tcgtggcgtc cttcgagcag cttggccgcg cgcgtcatcg catcgacgtc
     3181 gagccggtct ttgatccacc agacaaacgg cttcgcgaac gcggtgcgcc gcgtggcgat
     3241 ctggtagagg tagacgcgcg agacggcatc gtgacgcgcg tgaaaacggt cgctcgcgcg
     3301 cgacgccgca acgatgctga tgtcgtgcgg gagcaggtcg ttgatcttgc gcgagagcag
     3361 cgggggatcg acgcccttgt gcgtgcgcaa atgcgctacc tgcgctgccg cgtgaacgcc
     3421 cgaatcggtg cgcccggctc ctccgatgtc ggcgtcgcca agcacttgtt cggccgcccg
     3481 ctggaggtgc ccctgcacgg ttttcggcgt gttaccctgc gcctgccagc ccgagtaccg
     3541 cgtcccttcg tactcgatcg tgagcttata agtcggcatg cgtcccgcgt cgtatcaggt
     3601 gatcggagat ctgctcgcag tcctctggga cgacgggcac gaatcatact atccgctcga
     3661 agcgctccgc cgcgcctgcc cctgcgccgc ctgctccggc gagcccgatc tgttcggccg
     3721 catgtccagc ggcccgcccg cccgctacac gcccgcgtcg ttccagctcg acagcgtcat
     3781 tccggtcgga aactacgcgc tgcagccgaa ctgggccgat ggtcatacct acgggatgtg
     3841 gactcatgag agtctgcgcg ccttctgccc gtgcggatcc tgcggaaaga agtaacaccg
     3901 aggtatcctc ggggtacgtc gatcggcggg acgttgtcag ggcaaatcat ggtcatcgtg
     3961 cacgccgagc acgggaccga ttacgcatca tcaatacacg gcgcgccacg cgctccgaaa
     4021 tacaacgcga tatgaaaaag gaagccgaga cgagccacga cgcaaccaac gaagtagacg
     4081 acctgctgcc cgagtacgat tttagcggcg gtgctcgagg caagtactac cgccctggca
     4141 agacgacgat ccgcatgacg atcgatgaag atgtggcgcg atacttctac tcggcagagc
     4201 ttctcaacgc cgcccttcgc caattgattg ttgagggacg tgcgccggag ccacgtgatg
     4261 aggcgtcggg ctcatgacca tgcgaattac atgggatcca ctgaaggcgg ccgag
//
LOCUS       DATLVW010000862         5029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_205982, whole genome shotgun sequence.
ACCESSION   DATLVW010000862 DATLVW010000000
VERSION     DATLVW010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205982"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNN25_25255"
     CDS             complement(<1..189)
                     /locus_tag="VNN25_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94906.1"
                     /translation="MQRIVLSLSVALLTLVGSAASAATTTMPLSQVRKGMHGYGETVF
                     DGTRLERFDVEITGVLQNI"
     gene            complement(442..1122)
                     /locus_tag="VNN25_25260"
     CDS             complement(442..1122)
                     /locus_tag="VNN25_25260"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HXH94907.1"
                     /translation="MSYSVTTLDGERKPRRLPARELDPLEIRILGCLMEKQLATPEYY
                     PLTLNALVAACNQKSNREPVMEVGESDIQRSLDRLQDEKLVWKVLGGRATRWDHNLDL
                     TWHLDRPAKAILALLFLRGAQTPGELRGRSDRLHGFETIDDVEAMLQRLGHGDDALLR
                     ELPRRSGQKETRWMHRLGGAYVEPAVSVDDEPAVSGAPLSVRVQRLEDQVATLMTELH
                     ELKAKLGE"
     gene            complement(1192..3594)
                     /locus_tag="VNN25_25265"
     CDS             complement(1192..3594)
                     /locus_tag="VNN25_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HXH94908.1"
                     /translation="MAIKTESTSFTKDIQGRYLCNTYGEATAGGPFDIVIIGGGTFGA
                     AIAEHVWYRQKRAGGGLRTIVLEAGPFTLPEHVQNTGVLGLFDPADSVILNENNPQPE
                     PPRNEVWGVPWKSATPFKGLAYCVGGRSLYWGGWSPRLLDAEVATWPPEVVSDLKSGY
                     FDESTKQIGVDDTNDFIFGELHNALRRQLFDNIGTVAAAIPLAELPPSPLLKPGADPA
                     QLLGLTSPGSLSQDDLLNLLKLEAPLAVQARAPHAGFFPMNKFSTVPLLMKAARTAFA
                     DNADDAKKEFMLLPNTHVLTLRTVRNGDTWRVTRVETSQGPIDLAPDGVAIIALGTIE
                     SARLALVTFDGSGLPTLPRIGRNLMAHLRSNMTFRVPRTAIPGLSAAVQELQTSALFL
                     KGRVRKNGTLLGHFHLQITASGGASTVGSEDELFRKVPDIDFYQALQASTDTHVAIAI
                     RGIGQMSPDDLVNPDPSHSNVALDGVLRDEYGVRRARVTMAKSQTDLDLAAAMEAAMM
                     QVAQLFGAPATVTPAPDGLGTTHHETGTLSMGTDPAASVTDGDGRFHHTENLYAAGPS
                     LFPTIGSPNPMLTGIALARRTGDRIINPPPFVATTGFQSLVDNALTDWKMSTIRNQPG
                     RDNPGSFVLRRGALEARPGTDLGLFWYTKPAPSRFMLRLDWMMTAPDDNSGVFICFPD
                     PESKGYDNGAYVAVNFGFEVQIDEQARPDGSPIHRTGAIYSFQGPDLTRLNVRAVGEW
                     NAYEITADGTDITVSLNGQLVNQFHFSGDPQSPTRGQGSFIGLQTHTGRVRFRNLQWK
                     AV"
     gene            complement(3750..4247)
                     /locus_tag="VNN25_25270"
     CDS             complement(3750..4247)
                     /locus_tag="VNN25_25270"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXH94909.1"
                     /translation="MIRQVIGIGCLLIAAVVVNAVTPSQQADRVVIMKSARQLELLRS
                     GKVMRSYHVRLGSNPIGGKEREGDGKTPEGIYRIDSRNADSKYHRALHVSYPNAADAA
                     RARRMHVSPGGEIMIHGVPNRWRWLSFVFQRIDWTAGCIAVKDDEIEEIWRLVPNGTV
                     AEIRP"
     gene            4352..4939
                     /locus_tag="VNN25_25275"
     CDS             4352..4939
                     /locus_tag="VNN25_25275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94910.1"
                     /translation="MSGLRHIESFEESLKDGRFDRTSLYWSQLRFQQQSASLERRDVT
                     RRLDDFVRILKDHGPRAKDFAPRRESSFRPKEKSFQAKEKSFRLKEKSFRPKEKSFQP
                     KETSFRPKENSFQPKETSFQLKESSLRPRENSLRPKETSLRPRENSLPPKESFVAHGE
                     SFTLCRESLAPAGEDSGPSLKRFARVVSEKGRSPR"
BASE COUNT          935 a         1517 c         1594 g          983 t
ORIGIN      
        1 gatgttctgc aggacgccgg tgatctcgac gtcgaagcgc tcgagcctgg tgccatcgaa
       61 cacggtctcg ccgtacccat gcatgccctt ccggacctgc gagagcggca tcgttgtcgt
      121 tgcggctgac gccgccgaac cgacaagagt caacagcgcg acagaaaggg agaggacgat
      181 tcgctgcacg cgaggtctaa cagtggtgcg gaaggaggct atttcgtttt atgcgggtgt
      241 cgcggttcga gggtcgggtg tcggagaaac aatgttggaa cggtcgaaaa ggaatgattg
      301 ggttggtgtt tggggagttg gcggcggcga gcacactcga gacctcgcgc agcgcgttgt
      361 gctcagcggc tgaggacgcc aaaggacccg atgcgccatg aatgttaccg cgcatacgga
      421 tgacagtgaa cgtacgagcg cctactcccc cagcttcgcc ttcaactcgt gaagctcggt
      481 catgagagtc gcgacctgat cctccagccg ttgcacgcgg acggaaagtg gggcgcccga
      541 aactgccggc tcgtcatcga ccgaaacggc cggctcgacg tacgcaccgc cgaggcgatg
      601 catccagcgc gtctctttct ggccggagcg gcgcggcagc tctcggagga gcgcgtcatc
      661 gccgtgtccg agtcgctgca acatcgcttc gacgtcatcg atcgtctcaa agccgtgaag
      721 ccggtcgctg cgtccgcgca actcgcccgg cgtttgcgcg ccgcggagga agagcagcgc
      781 aagaatcgct ttggcgggcc ggtcgagatg ccaggtgaga tcgagattgt ggtcccagcg
      841 cgtggcccgg ccgccgagca ccttccagac gagcttctcg tcctgcagcc ggtcgagcga
      901 gcgctggatg tcgctctccc ccacttccat gaccggctcg cggttcgatt tctgattgca
      961 cgcggcgacg agtgcattga gtgtgagcgg gtagtactcg ggcgtggcga gctgcttttc
     1021 catcaggcag ccgaggatgc ggatctccaa cggatcgagc tcgcgcgcgg gcagacgacg
     1081 cggtttgcgc tcgccgtcaa gagtggttac ggagtaggac atggagagaa ctgtttcaca
     1141 cgaagacgcg gggtcttaat tttcgcggct tcgcgtcttc gcgtgaagat gctacaccgc
     1201 cttccactgc agattccgga accgcactct ccccgtgtgc gtctgcaaac cgatgaacga
     1261 tccctgaccg cgcgtcggtg actgtggatc gccgctgaaa tggaactggt tcacgagttg
     1321 gccgttcagc gagaccgtga tgtccgtgcc gtctgccgtg atctcgtagg cattccactc
     1381 gccgacggcg cgaacgttga gccgcgtcag gtcgggtccc tggaatgaat agattgcgcc
     1441 ggtgcggtgg atcggcgagc cgtcaggccg ggcctgctcg tcgatctgca cctcgaatcc
     1501 gaagttgacg gccacgtacg cgccgttgtc gtaacccttc gactccggat cggggaagca
     1561 gatgaagacg ccggagttgt cgtcgggggc agtcatcatc cagtcgagac gcagcatgaa
     1621 gcgcgacggc gcgggcttgg tgtaccagaa caacccgagg tccgtgccgg ggcgggcttc
     1681 gagcgcgccg cggcgcagga cgaatgaacc cggattgtcg cggcccggct gatttcggat
     1741 cgtcgacatc ttccagtcgg tgagcgcatt gtcgacgagc gattggaatc ctgtcgtcgc
     1801 aacgaaaggc ggcggattga tgatgcgatc gccggtccgc cgcgccagcg cgattcccgt
     1861 cagcatcgga ttcggcgagc cgatcgtcgg gaagagcgac ggtcccgcgg catagagatt
     1921 ctccgtgtga tgaaagcggc catcgccgtc ggtgacgctc gctgcgggat ccgttcccat
     1981 cgacagcgtg ccggtctcgt gatgcgtcgt gccgagcccg tcgggtgcgg gtgtcaccgt
     2041 tgccggcgcg ccgaacaatt gcgccacctg catcattgcc gcctccatcg ctgcagccag
     2101 atcgagatcc gtctgcgact tcgccatcgt cacgcgggcg cggcggacac cgtattcgtc
     2161 gcgcagcaca ccgtcgagcg ctacgttgct atggctcgga tcagggttca ccagatcgtc
     2221 cggcgacatc tgaccaatcc cgcggatggc gatggccacg tgtgtgtcgg tcgatgcctg
     2281 caacgcttgg tagaaatcga tgtccgggac cttgcgaaaa agctcatcct cggagccgac
     2341 ggtgctggcg ccgccgctgg ccgtgatctg aaggtggaag tgtccgagga gtgtgccgtt
     2401 cttcctgacc cgccctttga ggaagagcgc cgaggtctga agttcctgca cggccgcgga
     2461 cagacctggg atcgccgttc gcggaacgcg gaaagtcatg ttggagcgga gatgcgccat
     2521 caggttcctg ccgatgcgag ggagcgtagg aaggccgctg ccgtcgaagg tgacgagcgc
     2581 caggcgcgcg ctttcgatcg tgccgagcgc gatgatggca acgccgtcgg gcgcgagatc
     2641 gatcgggccc tggctcgtct ccacgcgcgt cactctccac gtgtcgccgt tgcgcacggt
     2701 tcgcagcgtc agcacgtgcg tattcgggag gagcatgaac tccttcttcg cgtcgtccgc
     2761 gttgtcggcg aacgccgtgc gcgccgcctt catcagcagc ggcacggtgc tgaatttgtt
     2821 catgggaaag aagccggcat gcggcgcgcg cgcttgaacc gccagcggcg cctcgagctt
     2881 cagcaggttg agaagatcgt cctgcgagag gctgccgggc gaggtgagac cgaggagctg
     2941 ggcaggatcg gcgccgggct tgagcagcgg cgatggcggg agctcggcca gcggaatggc
     3001 ggctgcaacg gtgccgatgt tgtcaaacaa ctgacggcgc agggcgttgt gcaactcgcc
     3061 gaagatgaag tcgttcgtgt cgtcgacgcc gatctgcttc gtgctctcat caaagtagcc
     3121 gctcttcaag tcgctcacaa cctcgggtgg ccaggtcgcc acttcggcgt cgagcagccg
     3181 aggcgaccag ccaccccagt acaacgatcg cccgccgacg cagtacgcca ggcccttgaa
     3241 cggcgtcgcg gacttccacg gcacacccca cacctcgttg cgcggaggct ccggctgcgg
     3301 gttgttctca ttgaggatca cggagtcggc cggatcgaag agcccgagga cgccggtgtt
     3361 ctgcacgtgc tcgggaagcg tgaacggccc cgcctcgagg acgatcgtgc gcagcccgcc
     3421 gcccgcgcgc ttctgccggt accagacatg ctcggcgatc gccgccccga acgtgccccc
     3481 tccgatgatg acgatgtcaa acggcccgcc agccgtggcc tcgccgtagg tgttgcaaag
     3541 atagcggccc tggatgtcct tggtaaatga agttgactcc gtcttgatgg ccatggttct
     3601 ttcctttgcc accgaatgat gatggggaaa gtccgttata cacgcgggcg tttcagcgtg
     3661 caaaaggtgg agcgggctct gctgaaccgc gatgcggaga cttcgcgcct tcgcgtcttc
     3721 gcgtgaaacg tccccgtctg gaacccagct tacggtcgga tctcggcgac cgtcccgttc
     3781 ggcacgagcc tccagatctc ctcgatctcg tcgtccttga ccgcgataca ccccgccgtc
     3841 cagtcgatgc gttgaaacac gaaactcagc cagcgccagc gattggggac tccatgaatc
     3901 atgatctcgc cgcccggcga cacatgcatg cgacgcgcac gagccgcgtc agcagcgttc
     3961 ggatacgaaa catgcagcgc ccggtggtac ttgctatcgg cgttgcggct gtcgatccgg
     4021 tagatcccct ccggagtctt tccgtcgccc tcccgttcct tcccgccgat cgggttggaa
     4081 ccgaggcgca cgtgatagct gcgcatcacc ttcccgcttc gaagcaactc tagttgcctt
     4141 gccgatttca tgatcacgac tcggtctgcc tgctgtgacg gtgtgaccgc gttgacgacg
     4201 accgcggcga tgaggaggca tccgataccg atgacttggc gaatcacggc atcaggatac
     4261 cgaatgcgcc gaacggccgt cgcggctgtc gtctccgtga ccttttccgg gctgattcgt
     4321 ctaagtagat atccagaaac ggaggcgagt catgtctggt ttgcgccata tcgagtcctt
     4381 cgaagagagt ttgaaggatg gccggttcga tcgaacgagc ctttactgga gtcagctccg
     4441 tttccagcag cagtccgcct cgctcgagcg gcgtgatgtc actcggcggc tcgatgattt
     4501 cgtccggatt ctgaaagatc acggcccgcg agcgaaagat tttgctcccc ggagagaaag
     4561 ttctttccgg ccgaaagaaa agtctttcca agcgaaagaa aagtctttcc gactgaaaga
     4621 aaagtctttc cgaccgaaag aaaaatcttt ccaaccgaaa gaaacttctt tccgaccgaa
     4681 agaaaattct ttccaaccga aagaaacttc tttccaactg aaagaaagtt ctctccgacc
     4741 tagagaaaat tctctccgac cgaaagaaac ttctctccga cctagagaaa attctctccc
     4801 acctaaagaa agttttgttg cacatggaga aagtttcact ctctgtagag aaagtcttgc
     4861 tccagctgga gaagattctg gtcccagtct gaaacgtttt gctcgggttg tctctgaaaa
     4921 aggacgctcg ccacgatgaa tgactgccgt gtgcggatgc tgtcttccgc cggcgtgcgt
     4981 gcccctccgg cagccttgtc gatggcatcg cggatggcgc gtcgccttg
//
LOCUS       DATLVW010000863         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_406144, whole genome shotgun sequence.
ACCESSION   DATLVW010000863 DATLVW010000000
VERSION     DATLVW010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406144"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VNN25_25280"
     CDS             <1..401
                     /locus_tag="VNN25_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXH94911.1"
                     /translation="DFISAMPQGYDTEIGESGVLLSGGQRQRLAIARALFKDPPILIL
                     DEATSALDTESERLVQGALEHLMQGRTTLVIAHRLSTIRSADKIVVLDKGEIVETGTH
                     EDLLARRGIYRKLYDLQFAEEERPVGSLAS"
     gene            407..970
                     /locus_tag="VNN25_25285"
     CDS             407..970
                     /locus_tag="VNN25_25285"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HXH94912.1"
                     /translation="MIAILGSLFIRALHATLRVRHVDAARLESQPQYILAFWHAHLLL
                     MLHSAWRKPIVVMISQSKDGEYMARVFDHYGVASARGSSTRGGTAALRAVIRAAHDGK
                     NIVFTPDGPKGPPLIAKEGVVHAAQATGLPIVPVAFAAQKKNFCARGTGWSSRFRSRG
                     RSSSTASRCSFPAMATSKSGGGAWKKR"
     gene            1043..1933
                     /locus_tag="VNN25_25290"
     CDS             1043..1933
                     /locus_tag="VNN25_25290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXH94913.1"
                     /translation="MITSMTGFGRAAGALSSRYFVAVTAKSVNHRFLETSVRIPEYLW
                     EAEAMVRAIASDTFARGKVDISIRVQRTAQPDYNVRINTQIANTVVPQLRAIAEELGL
                     GSTFSGGDLLRIPDLLQVEAIDTEITDDEREALATIVRSALAQMAAMRDHEGTSLRTD
                     ITTRLHTIRELQTRLASHRDEIRGELLASYQQRVQEIAKAAGIEINQDRIAQEVVIMV
                     EKGDVAEELTRLAHHVEQAEKAVSGSDAAGKKLDFLSQEMLREVNTMGSKSRSAALRT
                     LVVELKTEVERIREQVQNVE"
     gene            1923..2606
                     /gene="gmk"
                     /locus_tag="VNN25_25295"
     CDS             1923..2606
                     /gene="gmk"
                     /locus_tag="VNN25_25295"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXH94914.1"
                     /translation="MSSNFHPDGTLFIVSGPSGAGKTTLINTVREQLEPIGIRLYFSV
                     SHTTRNPRAGEVDGRSYHFVPPDQFAAMAARGEFIEWAFVHEQRYGTSKAEVLARLDR
                     GEDVILDIDYQGARQVAADAELKPRLLNIFIFPPSFEHLDRRLRDRGLNSEQEIQTRL
                     QKAVDEIDAGKEFYDYVIINDDLNVAAECLKATIIAKKLQTKTALEAITAMAGRLKEE
                     RDGRFARGH"
BASE COUNT          576 a          861 c          839 g          477 t
ORIGIN      
        1 acgatttcat ctccgcgatg ccgcagggat acgacaccga gatcggggag tcgggtgtgc
       61 ttctttcggg tggacagcgg caacggctcg cgatcgcacg agctctcttc aaagatccgc
      121 cgatcctaat cctcgacgag gccacttccg cgctcgacac ggagtcggag cggctggtgc
      181 aaggcgcgct cgagcatctg atgcagggga ggacgacgct cgtcatcgcg caccggctct
      241 ctacgatccg tagcgccgac aagatcgtcg tcctcgataa aggagagatc gtcgagaccg
      301 gtactcacga agatctcctg gcgcggcgcg gcatctatcg caagctctat gatcttcagt
      361 tcgcagagga agagcgtccg gtcgggtcgc tcgcgtcatg atgcgattga tcgccatact
      421 tggatcgctc ttcattcggg cactgcacgc cacgctgcgt gtgcgccacg tcgacgccgc
      481 aagactcgaa tcgcagccgc agtacatcct ggcgttctgg cacgcgcatc tgctgctgat
      541 gcttcactcg gcatggcgca agccgattgt tgtcatgatc tcgcagtcga aagatggcga
      601 atacatggcc cgcgtcttcg accactacgg cgtcgcgtcg gcgcgcggat catcgacgcg
      661 cggcggtacg gcggcgcttc gagccgtgat ccgcgccgcg cacgacggaa agaacatcgt
      721 cttcactccc gatggcccga aaggaccacc tcttattgcc aaagagggcg tcgttcatgc
      781 ggcgcaagcg accggactgc cgatcgtgcc ggttgcattc gctgcgcaaa aaaaaaactt
      841 ctgcgctcgt gggaccggat ggtcgtcccg tttccgttct cgcgggcgct cttcctctac
      901 ggccagccgc tgttcgttcc ccgcgatggc aacatcgaag agtggcggcg gcgcttggaa
      961 gaagagatga acgagctggc tgacagggcc gagaaactag tagaggagaa atgatgacgg
     1021 cctccgcctt catccttcaa tcatgatcac atccatgacc ggcttcggcc gcgctgccgg
     1081 cgcgctttcg tcgcgctact ttgtcgcggt gaccgccaag agcgtcaatc accgcttcct
     1141 cgaaacgtcc gtgcgcatcc ccgagtacct gtgggaagca gaagccatgg ttcgcgccat
     1201 cgcctcggac acctttgccc gcggcaaagt cgacatctcc atccgcgtgc agcgcacggc
     1261 ccaacccgac tacaacgtgc gcatcaacac gcagatcgcc aacacggtgg tcccgcaact
     1321 tcgcgccatc gccgaagagc tcggcctcgg gtcgacgttc tccggcggcg accttctgcg
     1381 cattcccgat ctgctgcagg tcgaagcgat cgataccgag atcacggatg acgaacgcga
     1441 ggcgcttgcc acaatcgtcc gttccgcgct ggcccagatg gctgccatgc gcgatcacga
     1501 aggaacgtcg ctgcgtacgg acatcaccac gcgcctccac acgattcgcg agctgcagac
     1561 gcggctggcc tcgcaccgcg atgagatccg cggcgagttg ctcgcttcgt atcagcagcg
     1621 cgtccaggaa atcgcaaagg cggccggcat cgagatcaac caggaccgca tcgcgcagga
     1681 agtcgtgatc atggtcgaga agggcgatgt ggccgaagag ctgacgcgcc tcgcccatca
     1741 cgtcgagcag gccgagaagg ccgtgagcgg cagcgacgcc gcaggcaaga agctcgattt
     1801 tctctcgcag gaaatgctgc gcgaggtgaa cacgatgggc tcgaagtcgc gctccgcggc
     1861 gctgcgtacg ctcgttgtag aattgaaaac cgaggtcgag cggatacgcg agcaggtaca
     1921 gaatgtcgag taactttcac cccgatggga cgttgttcat cgtctcgggc ccgtcgggtg
     1981 ccggaaagac gacgttgatc aacaccgtcc gcgagcagct cgaaccgatc ggcatcaggc
     2041 tctacttttc cgtctcgcac accacgcgca acccgcgggc gggcgaggtc gacggacgca
     2101 gttaccactt cgttccaccc gatcagttcg cagccatggc cgcccgcggg gaatttatcg
     2161 aatgggcctt cgttcatgag cagcggtacg gaacatcaaa ggccgaggtt ctggcgcggc
     2221 tcgatcgggg cgaggacgtc atcctcgaca tcgactatca gggagcccgg caggtcgcgg
     2281 cggacgccga gctgaagccc cggttgctca acatcttcat ctttccaccg tcgttcgagc
     2341 atctcgaccg acggcttcgc gaccgcgggc tgaacagcga acaagagatc cagacgcggc
     2401 tgcagaaggc ggtcgacgag atcgacgccg gaaaagagtt ttacgactac gtcatcatca
     2461 acgacgacct gaacgtcgcc gccgaatgcc tgaaggcgac gatcatcgca aagaaactac
     2521 agacgaagac agcgctcgaa gcgatcaccg cgatggccgg gcgtctgaag gaggaacgag
     2581 atggaaggtt tgccagaggg cattgacagt aaattccgtt acgttctgct cgtggccaag
     2641 cgcgccgagc agctcgtaca aggctccatg ccgaagagca agagcagaca cgccaagccg
     2701 acgcgcatcg ccatggaaga gatcgagaag aaccaggtga agtggcagct cac
//
LOCUS       DATLVW010000864         4187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_285138, whole genome shotgun sequence.
ACCESSION   DATLVW010000864 DATLVW010000000
VERSION     DATLVW010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285138"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VNN25_25300"
     CDS             complement(<1..1001)
                     /locus_tag="VNN25_25300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HXH94915.1"
                     /translation="MPFIVLSKLERFVPANIRKRGAEYARGGAVKIRARGSIDDLLMA
                     QVYGSDVYDVSLVYGERALWATCTCPYFERDLEICKHIWATVIRCEQTAVSFPGPVDD
                     LLSEGEAGFDDAVFSVDDEFDDEYVARPAPPRPTYLRPVPSRWRAQLGAVDSYARDAI
                     DGAGVPDEIIWVLDPEASGTSIQIEITSRGFKKNGERTKPKAVRLDPAVVSRVPDSRD
                     REILATLMASSYGWDSLDPSRSVGWPLAEYLVPKLVETGRLWIRTGKGEYASGVTFDA
                     GEPWQLKIQAVEGDGKYVLAASFHRGEETLAVHDPLAVTRGGFLIFRDSIARFADEGT
                     "
     gene            complement(1006..1932)
                     /locus_tag="VNN25_25305"
     CDS             complement(1006..1932)
                     /locus_tag="VNN25_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94916.1"
                     /translation="MPIRLSCALALLLLAQAVSAEEIPDSVLKAGDASITIQYSTKVS
                     DDFRKLVEAWVRDAADAVITVYKRYPVERVTIEVRVGNGGRVGGGMTFDGRLIKVRVG
                     SETTREAFRSDWVMTHEMFHLGFPDTDRTANWIGEGLSTYMEPLARARAGQLSAEEVW
                     GSMIDGFPKGLPAAGDQGLDHTPTWGRTYWGGALFWLLADIEIRRQTNGRKSLDDALR
                     AIVAEGGIGSSHWPEAKIFRRADAAIGKPILEKLDERLGEHPEKTDLAALWQQLGVSQ
                     KERTVLFNDSAPWASIRRAMTQKVSSTAIQSP"
     gene            complement(1995..2693)
                     /locus_tag="VNN25_25310"
     CDS             complement(1995..2693)
                     /locus_tag="VNN25_25310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXH94917.1"
                     /translation="MSSDGEGSVRMLKTARVPGQFFLVGSEDGLLDERPVHRVWIDAF
                     EIGVTQVTNSEYASFANATDRALPPTPFIDPEQPVVAVSWFDAVAYCDWLSGISGRCV
                     RLPAEAEWECAARGGTEGMRYPWGNELPLPDNDRFANGPEPVGRRGPNAYGLYDVCEN
                     VHEWCSDWYAPDFYAVSPERNPRGPESGKRRVSRGGSWRHRVKFSRCAARSSIPPEFQ
                     YADYGFRIACDTKP"
     gene            2805..3275
                     /locus_tag="VNN25_25315"
     CDS             2805..3275
                     /locus_tag="VNN25_25315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXH94918.1"
                     /translation="MAKTNAELVQDWINGTASPDPKSPIRLPPPIAITLPFRVSHVEP
                     GTCTVEMTTDPDTQANPMGTIHGGVLCTIADAAMGVAHWSGLAEGESFTSVDFRINFF
                     RPVWRDSLRATAKAIHSGRTLTYYACDIVRADGKLVAHATSTIMTLSGEAAKGR"
     gene            3357..3647
                     /locus_tag="VNN25_25320"
     CDS             3357..3647
                     /locus_tag="VNN25_25320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94919.1"
                     /translation="MPQSAVPMSEQIIPMSEQIIPMPERIIPMPEQIIPMSEQFIPMS
                     KQIIPMSEQIIPMSEQIIPMSERIVSMPEQIAPMPQCAVPISQSSVPAARRA"
     gene            complement(3988..>4187)
                     /locus_tag="VNN25_25325"
     CDS             complement(3988..>4187)
                     /locus_tag="VNN25_25325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94920.1"
                     /translation="RRRHAIVDDRLRVVRRARLQRRGMRAAVKKRERRSGRWRYEGGE
                     GSSNEDDISASAAFSGNSHEV"
BASE COUNT          841 a         1370 c         1226 g          750 t
ORIGIN      
        1 gtcccttcgt ccgcaaaccg ggcgatcgag tcgcggaaga tcaggaaccc gccgcgcgtg
       61 accgcgagcg gatcatggac cgcgagcgtt tcctcgccgc gatgaaacga agccgcaagg
      121 acgtactttc catcgccttc gacagcctga atcttcaatt gccacggttc gccggcatcg
      181 aacgtgactc ctgatgcgta ctcacccttg ccggtgcgaa tccagagacg ccctgtctcg
      241 acgagcttcg gcacgagata ctcggcgagc ggccaaccga ccgaccgcga cgggtccagc
      301 gagtcccatc cgtagctcga cgccatcagc gtggccagga tctcgcggtc gcggctgtcg
      361 ggaacgcggc tcacgacggc gggatcgagc cgtactgctt tcggtttcgt gcgctcgccg
      421 ttctttttga aaccgcgcga cgtgatctcg atctggatcg acgtcccgct cgcctcggga
      481 tcgagaaccc agatgatctc gtccggcacg ccggcgccgt caatcgcatc tcgcgcatac
      541 gaatcgacgg caccgagttg ggcccgccag cggctcggga cggggcgcaa gtaggtcggt
      601 cgcggcggcg caggccgcgc gacgtactca tcgtcgaatt catcatcgac agaaaagact
      661 gcgtcgtcga agccagcttc gccttcgctc agaagatcgt cgacggggcc cggaaaggag
      721 accgcagtct gctcgcatcg aatgaccgtc gcccagatgt gcttgcagat ctcgagatcg
      781 cgctcgaagt agggacaggt gcatgttgcc cagagagcgc gctcgccgta cacgagggag
      841 acgtcgtaaa catcgctccc gtaaacctgc gccatcagca gatcgtcgat cgatccgcgc
      901 gctctaatct tgaccgcgcc gccgcgcgcg tactccgccc cccgcttgcg aatgttggct
      961 ggaacgaaac gctcgagttt cgagagaacg atgaatggca cggagttatg gcgattgtat
     1021 cgccgtgctg gagactttct gagtcattgc gcgccggatg cttgcccatg gcgcgctgtc
     1081 gttgaacaag acggtgcgct ctttctggct gaccccgagt tgttgccaga gagcagcgag
     1141 atcggttttc tccgggtgtt cacccagacg ttcgtcgagc ttctcgagga tcggctttcc
     1201 gatggcggcg tccgcgcggc ggaagatttt cgcctccggc cagtgactcg accctatccc
     1261 tccctcggct acgatcgcgc gcaaggcatc gtcaagcgat ttccggccat tcgtctgccg
     1321 ccggatctcg atgtcggcga gcagccagaa gagcgctccg ccccagtagg tacggcccca
     1381 cgtcggggta tggtcgagac cctgatcgcc ggcagcaggg agacctttgg gaaagccgtc
     1441 gatcatgctg ccccagactt cctccgcgct aagttgcccg gcacgagctc gggccagcgg
     1501 ctccatgtag gtcgacaaac cctcgccgat ccagtttgcg gtccggtcgg tgtcgggaaa
     1561 gccgaggtga aacatctcgt gggtcatcac ccagtcggac cggaaggcct cgcgagtggt
     1621 ctccgatccc acgcgcactt tgatcaaacg gccgtcgaag gtcattccgc cgccgacacg
     1681 accaccgttc ccaacgcgca cctcgatggt cacccgctcg acgggataac gcttgtagac
     1741 ggtgatgacc gcatccgcgg cgtcgcgaac ccacgcttct acaagcttgc gaaagtcatc
     1801 cgagaccttt gtggagtact gaatggtgat tgatgcgtca ccggccttca gcacagagtc
     1861 aggaatctcc tctgcgctta cggcctgggc aagaaggaga agggcgagag cgcacgagag
     1921 tcggattggc atcaagagtc gattctgacg caaataccgc acctgttcga taccgttgaa
     1981 acaacgccca gacttcacgg cttcgtatcg caagcgatcc tgaatccgta gtcggcatac
     2041 tgaaactccg gcggaatgct cgagcgcgcg gcacagcggc tgaacttcac tcgatgacgc
     2101 caggagccgc cacgcgacac gcggcgcttg ccgctctccg gaccgcgcgg attccgctcc
     2161 ggcgaaacgg cgtagaagtc cggcgcatac caatcgctgc accactcgtg aacgttctcg
     2221 cagacgtcat agaggccgta ggcgttcgga ccgcgtcgtc cgactggctc cgggccgttc
     2281 gcgaaacgat cattatctgg caacggcaac tcattccccc acggatagcg catcccttcc
     2341 gttccgccgc gcgccgcaca ctcccactcc gcctcggccg gcagacgcac gcaacgcccg
     2401 gagatcccgc tcagccaatc gcaatacgcc accgcatcga accacgacac agccacaacc
     2461 ggctgctcag gatcgatgaa cggcgttggc ggcagcgcgc ggtccgttgc atttgcgaac
     2521 gatgcgtatt cgctgttggt cacctgggtg acgccgatct cgaaggcatc gatccagact
     2581 cgatgcaccg gccgctcgtc gagaagacca tcctccgatc ccacgaggaa gaactgcccc
     2641 gggacgcgcg cggtcttcag catcctcaca ctcccctcgc catcagaaga catgccacat
     2701 attctgcaca tcgttcgatg gaaccaggct gaatggcggc gcatcgacac gggatacgct
     2761 tgcccgatac tcggatgctc gcaacgagcg tgaaggagca aatgatggcg aagaccaacg
     2821 cagagctggt gcaggattgg atcaatggca cagcgtcgcc ggatccaaaa tcgccgatcc
     2881 gtctccctcc accgatcgcc atcacgctcc ccttccgcgt cagccacgtc gagccgggca
     2941 cgtgcacggt ggagatgacc accgaccccg acacgcaggc caatccaatg ggcacgatcc
     3001 acggcggcgt cctctgcacg atcgccgacg ccgcgatggg agtcgcgcac tggagcggac
     3061 tggccgaggg agagtcattc acgagcgtcg attttcgaat caatttcttc cgcccggtgt
     3121 ggcgcgactc gctccgcgcg accgcgaagg cgatccatag cggccgcacc ctcacctact
     3181 acgcctgcga catcgtccgc gccgacggca agctcgtggc ccacgccacg agcacgatca
     3241 tgacgctgag cggggaggcg gcgaagggac gctgacgcgc gcgatgctca cgcaaccgga
     3301 aaaaagagcg ccaaaagaca ggagagggcg gtaataagtc ggaatgtttt gttccgatgc
     3361 cgcaatccgc ggttccgatg tcggaacaga tcattccgat gtcggaacaa atcattccga
     3421 tgccggaacg aatcattccg atgccggaac agatcattcc gatgtcggaa caattcattc
     3481 cgatgtcgaa acagatcatt ccgatgtcgg aacagatcat tccgatgtcg gaacagatca
     3541 ttccgatgtc ggaacgaatc gtttcgatgc cggaacaaat cgctccgatg ccgcaatgcg
     3601 cggttccgat atcgcaatcg tcggttccgg ccgcgcgacg ggcatgacgc agctcagact
     3661 ctcgccatgc ctcacgcgaa ctgcctgaca acacggcaga tgcgctgacg tcatcctcgt
     3721 tggaacgccc cctcatccgc cttcggcacc ttctccccca cggagaagcg tgggggagaa
     3781 ggcgctcgat tggaaagaag ggatcaattt gtcgagagta cgagaaatgt gggttcgccc
     3841 cgcatttttc atgcgtttgc accgacttac tacacccgtc ctttctcagg agtgctttcc
     3901 catcgagcgc cttctccccc cgcgcttttt gcggggggag aaggtggcga agccggatga
     3961 ggggggcgta atgcgcagag cgatcaacta cacctcgtgc gaattgcctg aaaacgcggc
     4021 agatgcactg atgtcatcct cgttggaact cccctcaccg ccttcgtacc gccagcgccc
     4081 cgagcgacgt tcgcgcttct tcaccgctgc gcgcatcccg cgccgctgca atcgagcgcg
     4141 acgaacaact cggaggcgat cgtccacgat ggcgtgccgg cgccgcc
//
LOCUS       DATLVW010000865        15504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_206084, whole genome shotgun sequence.
ACCESSION   DATLVW010000865 DATLVW010000000
VERSION     DATLVW010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15504
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206084"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..427)
                     /locus_tag="VNN25_25330"
     CDS             complement(128..427)
                     /locus_tag="VNN25_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXH94921.1"
                     /translation="MKKTFTCSLLFALALPAVALADGAAIYKTKCTPCHGADGSGQTP
                     VGKNLKVRDLRSAEVQKLSDADITKVLTDGKGKMPKSKLEAADIKSVIAYIRTLK"
     gene            complement(594..974)
                     /locus_tag="VNN25_25335"
     CDS             complement(594..974)
                     /locus_tag="VNN25_25335"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXH94922.1"
                     /translation="MNKTTVAIAGVSLIALFFAAYAAMRSSAVTAPEAPAAVATTSAA
                     PTPAPIKEAAVPTGASLKAIPRINATTLHDRMARGEVVVIDVRDIDSYTASHIAGAMH
                     IPLSRIEGEIPYLKGSKPIVTYCT"
     gene            complement(971..1660)
                     /locus_tag="VNN25_25340"
     CDS             complement(971..1660)
                     /locus_tag="VNN25_25340"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXH94923.1"
                     /translation="MNTDASAVLAAAVTEELRWDPLVDVERINVSAEDSKITLHGVVR
                     TYAQKCRAEKIAGEIRGVVDVKNELDVRLAIGSYRTDDGLEQLAASIMQNHSALCDPL
                     PRVTAHDGWLTLSGVVTTEAQKRAAEDALRDLTGIRGIANCIEVAPPREDAGDAGVFL
                     AAVLRRGKLGVNDLKVEVNGGAIAIDAKVTSCAERDALIELASCRRGIVSVEDRVVVQ
                     PTPAQPNSRTS"
     gene            complement(1768..2385)
                     /locus_tag="VNN25_25345"
     CDS             complement(1768..2385)
                     /locus_tag="VNN25_25345"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HXH94924.1"
                     /translation="MRISIFAGDVADAPAEALCTSTNPRLSLVMGTGASIRGRGGFEI
                     LRACKAIVKAEFSRTGIHGLSAGSVHTTAAGQLPAKVVIHCVASDAAHRSSAAIIRSC
                     VKNALAAADIAGCTSIAMPLFGTGHAHFKTDQALAAIADALRDAVTEVRHILLVVRDP
                     DYVSDAVQIMERLIPGCEVDVQRGPSETSERPSLWPSSWGGRDEI"
     gene            complement(2382..5915)
                     /gene="nifJ"
                     /locus_tag="VNN25_25350"
     CDS             complement(2382..5915)
                     /gene="nifJ"
                     /locus_tag="VNN25_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HXH94925.1"
                     /translation="MIDGNEAAARVAYAMSEVIAIYPITPASNMGEWADDWAAQKRPN
                     LWGTVPSVVEMESEAGAAGAVHGALQTGSLATTFTASQGLLLMIPNMYKIAGELTPAV
                     FHVAARSLATHALSIFGDHSDVMAARGTGWAMLCSASAQEAHDLALIAHAATLECRIP
                     FLHFFDGFRTSHEIAKVTLLNDDQMRSMIDLELVFDHRSRALTPDHPVMRGTAQNPDV
                     FFQSRERANQFYTECPTIVAETMERFAKRTGRRYAPYEYHGSPHAERVIVAMGSACET
                     IGETVDALNESGENVGVVNVRLFRPFDVTRFVRSLPASVRAIAVLDRTKEPGSAGEPL
                     YLDVVTALHEAGRRIPIVIGGRFGLSSKEFTPAMAKGVFDELDATHPRQHFTVGIHDD
                     VTGLSIAFDPSFTVERDEMVRAMFYGLGSDGTVGANKNSCKIIGDTGRYAQGYFVYDS
                     KKAGAVTVSHLRFGPDPIHAPYLIERASLVACHQASLMPRNDVLKNLAHGGTFLLNAP
                     ITSSDELPEHIRTTLAEKEARLFVIDASRVARESGMGNRINTIMQVAFFACANVMPFD
                     EALDAICNAVRKTYARKGEAVVQKNLKAIDATLANLREFTISSADATTTGRIDHVHAP
                     EFVRDVLFEIIAGRGDDLPVSAMPCDGTFPTATAQYEKRNLAQEIPAWEAAACIQCGK
                     CAMVCPHAAIRIKAYDPSILETKPASFKSLTPMHKDWAGLAYTIQVAPEDCTGCQICV
                     DVCPARSKTNKEQMALVMQPQAPLLESERANWDFFLGIPERDRRSITPGLIRHQQLQQ
                     PLFEFSGACAGCGETPYLKLMSQLFGDRAVVANATGCSSIFGGNLPTTPWSKNSEGRG
                     PAWANSLFEDNAEFGLGFRLSLDKQREYAIEILKRLAPVLGDSLADEIINCAQKNEVE
                     IFEQRQRVELLKVRLRRMELSMRMHKLDSTDEQQLLAIADALVKKSVWIVGGDGWAYD
                     IGFGGLDHVLASGRNVNILVLDTEVYSNTGGQMSKATPRGATARFAAAGKPSAKKDLG
                     LMAMTYGNAYVASVAMGARDEHTLKTFIEAEAYDGPSLIIAYSHCISHGIDMTTALQH
                     QKSIVESGQWLLYRFNPDRAARGENALQLDSRAIKRPVEEFMKTENRFHDLSAENVAS
                     VQHDVDARWRMYSYLSTMQPAPAAAPEPGKG"
     gene            complement(6176..6979)
                     /locus_tag="VNN25_25355"
     CDS             complement(6176..6979)
                     /locus_tag="VNN25_25355"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HXH94926.1"
                     /translation="MSVVTEGSASSAFPRRVALRPLALPSEHGGWSMLFEPIVLGILV
                     APSLAGSLIAAAAVLAFLARHPLRLFVQDARRRKRYARTVACAWLAVAYAGMSAIALI
                     SAVAMSGSRFLLPLVAGALLASIQFALDVRQRGRSFVAEICGACATATTAAAIATAAH
                     RSWSVALPLALLAASLTLPAILFVRAVLRHERRTTTMVLHGAAVIGAAILWQRGLTPA
                     VTIIVMAMLLVRAAIGLREKVAPPAKIIGMREAIVGAAAVLVIAAGYLS"
     gene            7199..9100
                     /locus_tag="VNN25_25360"
     CDS             7199..9100
                     /locus_tag="VNN25_25360"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXH94927.1"
                     /translation="MRTFVIATVFLFAAALPVRSQLTNTDCLSCHDNKELTKDIGGGK
                     TVSVSVDPKKFAASVHGPLDCTNCHADVKAYPHDPAPKAVDCASCHPDSVAAWDTSLH
                     AKAVKSGVARGAKCADCHGPAHEVLPSSDPKSKTAHGAIPATCSACHGQKFVMAKAGL
                     SNQSAISYEASVHGRAVANGSAKAAVCTDCHDHHAVRPANDPLSGIFKFNVARTCGKC
                     HATTAAHYAESIHGKALARGNWNAPVCTDCHGIHTISRAGDAVNGPRSSCERCHAGVR
                     LTKEFGVAGTRVATYQSSYHGLARQMGSTTAADCASCHTAHDVLPSSDPRSAINPRNL
                     QKTCGKCHQGAQINFTQGKIHRVDGDVSDTPSRIVTWIKRIYIVLILLTIGFMLLHNA
                     LVWWKKAVAARKGRGHTVERMNLNQRIQHMVMGTSFVILVISGFALAWPNSYFAALFG
                     PSEQLRRMVHRIAAVVMIAIGLYHLAYMVFTREGRKGLRDLWLRFSDGGDVIAVFLYY
                     LGLSKKKPQFGRFSYAEKVEYWAGMWGTIVMALTGLMIWYSVAVATWIPRWWIDVATT
                     VHYYEAILATLAILVWHFYHVIFDPDVYPMNWAWFDGKMSEEQFKEEHPKAVDSAETH
                     TTTSVEGKD"
     gene            9238..10584
                     /locus_tag="VNN25_25365"
     CDS             9238..10584
                     /locus_tag="VNN25_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HXH94928.1"
                     /translation="MGILTWIIAPIILIFGIVVYIGGMLLERRRRRRLAPGEIPQYPR
                     IDLNERRTRVIIVSTAVGLILFVTMSVIGSYQAYRYTDSDAFCGTACHKVMTPEYTAY
                     QASPHAHVGCAGCHIGPGAGWFVRSKLSGSYQLYSVIAHKYPRPIPSPVENLRPAQET
                     CEQCHWPAKFFGAQLKVFDHYAYDETNTPKEVRMLIKTGGGSASGGQTSGIHWHMNIA
                     NEITYIATDKQRQVIPWVRVKDRNGNVTEYKSADSKLTDAQLATATKRRMDCVDCHNR
                     PTHIYRSPDRAVDTAMFTGKIDSTLPFIKQQSVAVLAAEYETTDAAVNAIAKAIPAYY
                     QKTYPDIYKAKRVQVAGATAAIQDIFRAIRFPEMRVDWRTHPDNLGHFATMGCFRCHD
                     DQHVSKDGKKISKDCQICHTVLPDATANASPTFAHPVDIGDLRGVNCSDCHTGGGM"
     gene            10605..10925
                     /locus_tag="VNN25_25370"
     CDS             10605..10925
                     /locus_tag="VNN25_25370"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXH94929.1"
                     /translation="MSRKSVAIILSVAVILIAAPAAFAAPDGGALYKAKCASCHGADG
                     GGMTPMGKAMKLRDLRSADVQKLTDAELTKVTADGKGKMPAYKGKVSDADITALVAYM
                     RTLK"
     gene            10967..11356
                     /locus_tag="VNN25_25375"
     CDS             10967..11356
                     /locus_tag="VNN25_25375"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXH94930.1"
                     /translation="MSYHSGVLDPPAESLPDIGDDPRFHAALAMLAALDYFEASDEFE
                     ELFFEAVRDEVEFARVFLQLAAGGVHLERRQWRAATQRLDEAVRAIARVTNDRGYDLA
                     RMADELRTLIEQVRQGSAFEWPRVLRR"
     gene            11400..13088
                     /locus_tag="VNN25_25380"
     CDS             11400..13088
                     /locus_tag="VNN25_25380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HXH94931.1"
                     /translation="MTPGAVALAKTAHRENVPPDVILIHPPSVFDFRERAIVYGPISD
                     VIPSTPVFEMYPVGFLTLSAYLRRHGYSVRIVNLALRMMRSRRFQPERFLRKFHPKLF
                     GIDLHWLPHAHGGPAVAALLKKLHPEIPIVFGGISATCFHRELIEDPAVDFVLRGSVT
                     EPSLLALVRELEGERRFELVPGLTWKQDGEVRVNPDAVLPTSLDEYDFDLGAMIRSVA
                     RHLDFWTSVPFQAWWRHPITAVFTVRGCARRCITCGASNAAFRRFMPGHHPLLRGPEA
                     IAKQVRQLAAITRTPIFLVGDLRDGGVSYAHDVIDALARTPVSNRIVFEFFDPPDAEL
                     VARIDASIRSWGAELSPESHDESIRGLLGKARFTNARMIQAIESVMASRCESLDLFFM
                     IGLPGQTTANVTETVDAIEELFIRFDHRLSAFITPMAPFVDPGSDGFADAEAHGYRLR
                     ARTLAEHGALLETSDWESMLNYETDWMTRREIVEATYNAAKRLNDLKRRYGRISDQRA
                     GRVEARRSAAHRLGGETHAVSEGTLNDKSELFQPGALLRNFRVGGILRLLFGRA"
     gene            complement(13104..14006)
                     /locus_tag="VNN25_25385"
     CDS             complement(13104..14006)
                     /locus_tag="VNN25_25385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXH94932.1"
                     /translation="MESLVPRLIVVPTDFSAPASHAVRYASALGERFGAHLLVVYADT
                     FVPPFDFTGSGVGVPTAEPESLIAAAREQLALHAERNISARVPYDIMVIVGHPMESIT
                     EAARDSGANLVVMGTHGRSGIRRLLTGSVTEAVMRTSPVPVIAVNSSASENASVGRIL
                     CAVNFTPACSAALRQAGALADDDKAPILLVHGLDDQELQPSIQELIRLHEWAPPELAG
                     RCQLKTAPRRGLAEEILDLALATAADLIVIDVSADRSPADNLRGSIAERIVQQSRCPV
                     LTVNAHAAQSHLALGHAREQLAAI"
     gene            complement(14137..14562)
                     /locus_tag="VNN25_25390"
     CDS             complement(14137..14562)
                     /locus_tag="VNN25_25390"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH94933.1"
                     /translation="MILVDTSGILAALFSDQNWHDECAEALLNATPPLIISPFVLAET
                     DYLIQKFGGVDAETLFLAEIGRRAYELATFDEDEVEAARHLITKYRPLGIGLADASIA
                     VLAVRYDTLDILTLDERHFRAIRPAPRKSFRILPADSLR"
     gene            complement(14559..14783)
                     /locus_tag="VNN25_25395"
     CDS             complement(14559..14783)
                     /locus_tag="VNN25_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HXH94934.1"
                     /translation="MKKTTIYLPDELKKRLENLARTSGRPEAQVIRDAISAATATTTS
                     PAPRIPLMKRGLGDPSIAENVDALLERFGR"
     gene            14891..15268
                     /locus_tag="VNN25_25400"
     CDS             14891..15268
                     /locus_tag="VNN25_25400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94935.1"
                     /translation="MKHRIARLMVVVAVAAIGIQSPAYAVKGYSAMSESENFHSVTRT
                     KVFKHRIAAPWTAARLKVAMRVDAGGATVRLIDAKGATRWEKTFEKGAVSIDQTFPGN
                     GDWQVELRLEDATGRYEIHLIAV"
     gene            complement(15292..>15504)
                     /locus_tag="VNN25_25405"
     CDS             complement(15292..>15504)
                     /locus_tag="VNN25_25405"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HXH94936.1"
                     /translation="VRVTFKDEKFSIPLTAKGMQARAEGVTTIKTLSKAEADHLAEEG
                     ATLKRNEDGTADEVSFVATGVELTRQ"
BASE COUNT         2876 a         4736 c         4916 g         2976 t
ORIGIN      
        1 gggagaagtc gctcgatgga tactcaccgc gagcgggaga aatcagggtg acggcgcttg
       61 ttgcgcttga cgggtctctt ggacggagca tgcttctccg acccccgacc cccgactccc
      121 cacaccccta cttcagcgtg cggatatacg cgatcaccga cttgatgtcg gccgcttcga
      181 gcttgctctt cggcattttg cctttgccgt cggtgaggac tttcgtgatg tcggcgtcgc
      241 tgagcttctg gacttccgcg gagcgaaggt cgcgcacctt gaggttcttg ccgacgggcg
      301 tctggccgct gccatccgcg ccgtggcagg gggtgcattt cgtcttgtag atcgcggcgc
      361 cgtcggcgag agccaccgcc ggcagtgcga gagcaaacag cagggagcag gtgaatgtct
      421 tcttcatgtt gatcaaactc gtttctcctt tgtaagcaac actacggttc gcggccctcg
      481 cgaattgcaa agcccgctgt tttccacgaa tcgagaccgc cggccagcgc cgaggcttcg
      541 atttttgctt tcctcaagta gaacgccgca ccggcgctcg attcctcgtc gggtcacgtg
      601 cagtacgtga cgatcggctt ggatcctttg agatacggaa tctctccctc gatgcgggac
      661 agcgggatgt gcatcgcgcc cgcgatgtga gaggcggtgt aggaatcgat gtcgcgtacg
      721 tcgatgacga cgacctcgcc gcgcgccatc cgatcgtgca acgtggttgc gttgatccgc
      781 gggatcgctt tgagcgacgc acccgtcgga actgcggcct ctttgatcgg tgctggtgtc
      841 ggtgccgccg aggtggtggc gacggccgcg ggcgcctcgg gcgcggttac tgcgctgctg
      901 cgcatcgctg catacgccgc gaagaaaagg gcgatcagac taacgccggc aatcgctacg
      961 gttgtcttgt tcatgatgtc ctcgagtttg gctgcgccgg agttggctgc acgacgacac
     1021 gatcctcgac gctcacgata ccgcgtctgc aggatgccag ctcgatcaac gcatcgcgct
     1081 cggcacacga ggtgactttg gcgtcgatgg cgatcgcgcc gccgttaacc tcgactttga
     1141 gatcgttcac gccaagtttt ccccgtcgca ggaccgcggc gaggaacacg ccggcgtcac
     1201 cggcatcctc cctgggcgga gcgacttcga tgcagttggc gatgccacga atgccggtga
     1261 gatcgcgcag cgcatcttcg gcggcacgtt tctgcgcctc ggtcgtgaca acgcctgaga
     1321 gtgtcagcca tccatcgtgc gcggtgacgc gcggcagagg atcgcaaaga gcgctgtgat
     1381 tctgcatgat gctcgcagcg agctgttcga gaccatcgtc cgttcgatag ctgccgatgg
     1441 ccagccgcac gtcgagctcg ttcttcacat cgacgacgcc acggatctcg cctgcgattt
     1501 tctcggcgcg gcacttctgc gcgtaggtgc gcacgacgcc gtgaagagtg atcttcgagt
     1561 cctcggcaga aacattgatc ctctccacgt cgacaagcgg atcccaccgc aactcttcag
     1621 tgacggcggc ggccaggacc gctgaggcgt ccgtgttcat aggtcgtcga accatagcgg
     1681 ctgcgaggcg ggcgccagaa ggaatcgggt cacgaataga tgtgacgcgc atcgcatcct
     1741 cccgaggaca tctgctgttc gtaggtgtca gatctcatcg cgtccacccc aactcgaggg
     1801 ccacaagctc gggcgctcgc tggtttcgga cggtcctcgc tgcacgtcga cctcacatcc
     1861 aggaatgaga cgctccatga tttgaactgc atcgctcaca tagtcgggat cgcggactac
     1921 gagaagaatg tggcgaactt cggtcacggc gtcgcgcagc gcgtccgcga tcgctgccag
     1981 cgcctgatcg gttttgaagt gcgcgtggcc ggtgccgaac agcggcatgg cgatgctcgt
     2041 acaacccgct atgtccgccg cggcgagagc gtttttcacg caactgcgaa tgattgcggc
     2101 cgatgaacga tgcgcggcat cactggcgac gcaatgaatg acgaccttcg ccggcaattg
     2161 tccggcagcc gtcgtgtgta cggagccggc gctcagtccg tggattcctg tacgcgagaa
     2221 ttccgcttta acgatcgctt tgcatgcgcg gagaatctcg aatcctccgc ggccgcgaat
     2281 cgacgcgccg gtccccatca ccagcgagag tctgggattc gtcgatgtgc agagcgcctc
     2341 ggcaggcgcg tcggccacat ccccggcaaa gatcgagatc ctcatccttt ccccggttcg
     2401 ggagccgctg ccggtgcggg ctgcatggtg ctgaggtacg aatacatccg ccagcgggcg
     2461 tcgacatcgt gttgcacgct ggccacattc tcggcgctga gatcgtggaa gcggttctct
     2521 gtcttcatga actcctcgac ggggcgcttg atcgcgcgcg aatcgagttg cagtgcgttc
     2581 tcgccgcgcg cggcgcggtc gggattgaat cgatagagca gccactggcc cgactcgacg
     2641 atcgacttct gatgctgcag cgccgtcgtc atgtcgatgc cgtgcgaaat gcaatggctg
     2701 tacgcgatga tcagcgacgg cccgtcatac gcctcggcct cgatgaacgt cttgagcgtg
     2761 tgctcgtcgc gcgcgcccat cgccacgctg gccacgtacg cgtttccgta cgtcatcgcc
     2821 atcaagccga ggtctttctt cgcggaaggt ttgccggcgg cggcgaagcg tgcggtagca
     2881 ccgcgtggcg tcgccttcga catctgcccg cccgtgttcg aatacacctc ggtatccagg
     2941 acgaggatgt tgacgttgcg cccgctggcg agcacgtgat cgagcccgcc gaatccaatg
     3001 tcgtacgccc atccgtcgcc gccgacgatc cagacgctct tcttcaccag cgcatcggcg
     3061 atggccagga gctgctgctc gtccgtgctg tcgagtttgt gcatgcgcat cgagagctcc
     3121 atgcggcgca ggcggacttt gagtagctcg acgcgctgac gctgctcgaa gatctctacc
     3181 tcgttcttct gcgcgcagtt gatgatttcg tcagcgaggc tgtcgccgag cacgggtgcg
     3241 agacgcttga ggatctcgat ggcgtactcg cgctgcttgt cgagcgagag gcggaagccg
     3301 aggccgaatt cggcgttgtc ttcgaagagg gagttcgccc acgccggccc gcggccttcc
     3361 gagttcttgg accacggcgt cgtaggaaga ttgccgccga agatcgacga acagccggtg
     3421 gcattggcga ccacggcgcg gtcgccgaag agctgcgaca tcagtttcag gtacggcgtt
     3481 tcgccgcagc cggcgcaggc gcccgagaac tcgaacagcg gctgctgcag ttgctgatgg
     3541 cggatgagtc ccggcgtgat cgagcggcgg tcgcgctcgg ggatgccgag gaagaagtcc
     3601 caattggcgc gctcggattc gagcagggga gcctgcggct gcatcaccag cgccatctgt
     3661 tccttgttcg tcttgctacg cgcaggacag acatcgacgc agatctggca gccggtgcag
     3721 tcttcgggcg cgacctgaat cgtgtaggcc aggccggccc aatccttgtg catcggtgtc
     3781 agcgacttga acgacgccgg ctttgtctcg aggatgctgg gatcgtaggc cttgatgcgg
     3841 atggcggcgt gcggacagac catcgcgcac ttgccgcact ggatgcaggc cgcagcctcc
     3901 cacgccggga tctcctgcgc caggtttcgt ttctcgtact gcgcggtggc ggtggggaag
     3961 gtgccgtcac acggcatggc gctgaccggc aggtcgtcac cgcgtccggc gatgatctcg
     4021 aagagaacgt cgcgcacgaa ctcgggagca tgaacgtgat cgatgcgtcc ggtggtggtg
     4081 gcatcagcgg agctgatggt gaactcgcgc agattcgcca gcgtggcgtc gatcgctttc
     4141 aagttcttct gcacgaccgc ttcgcctttg cgtgcatacg tcttgcgcac ggcgttacag
     4201 atggcgtcga gcgcctcatc gaacggcatc acattggcgc aggcaaagaa ggccacctgc
     4261 atgatcgtgt tgatgcggtt gcccatgccc gactcgcgcg caacgcgcga ggcatcgatc
     4321 acgaaaagcc gggcctcttt ttccgcgagc gtagtgcgga tgtgctcggg cagctcgtcg
     4381 gaggatgtga tcggagcatt gagcaggaac gtgccgccgt gcgcgaggtt cttgaggacg
     4441 tcgttccgag gcatgagcga agcctgatgg caagccacga gtgaggcgcg ctcgatcagg
     4501 tacggcgcgt ggatcggatc aggcccgaag cggagatgcg agacggtcac cgcgccggcc
     4561 ttcttcgagt cgtagacgaa gtagccctgc gcgtatcggc cggtgtcgcc gatgatcttg
     4621 caactgttct tgttcgcgcc gacggtgcca tcggagccga gtccgtagaa catcgcgcgc
     4681 accatctcat cgcgctcgac ggtgaacgac ggatcgaagg cgatgctcaa ccccgtgacg
     4741 tcgtcgtgga tgccgacggt gaagtgctgg cgaggatgcg tggcgtctag ttcgtcgaag
     4801 acgcctttgg ccatcgctgg cgtgaattct ttcgaggaga gaccgaagcg gccaccgatc
     4861 acgatgggga tccggcgtcc ggcctcgtgc agcgccgtga cgacgtcgag gtagagcggc
     4921 tcaccggcgc ttcccggctc cttcgtgcga tcgagtacgg caatcgcacg gacgctcgcc
     4981 ggcagcgatc gcacgaagcg cgtgacgtcg aacggacgga agagtcgcac gttgacgacg
     5041 ccgacgtttt cgccggattc gttcagcgcg tcgaccgttt cgccgatcgt ctcgcacgcc
     5101 gatcccatgg ccacgatgac gcgctcggcg tggggtgagc cgtgatactc gtacggcgcg
     5161 taacggcggc cggtgcgctt cgcgaaacgc tccatggtct ccgcgacgat cgtgggacat
     5221 tcggtataga actggttggc ccgctcgcgc gactggaaga agacgtcggg attctgggcg
     5281 gttccgcgca tgaccggatg gtcgggtgtg agcgcgcgcg aacggtgatc gaagacgagc
     5341 tccagatcga tcatcgagcg catctgatcg tcgttcagca acgtgacctt cgcaatctca
     5401 tgcgacgtcc ggaatccgtc gaagaagtga aggaacggga tgcggcactc cagcgtcgca
     5461 gcgtgggcga tgagggcgag gtcatgcgcc tcctgcgcgc tggccgagca gagcatcgcc
     5521 catcccgtgc cgcgcgccgc catcacgtcg ctgtgatcgc cgaagatcga gagcgcgtgc
     5581 gtggccagtg accgcgcggc cacatgaaag acggccggtg tcagttcgcc ggcgatcttg
     5641 tacatgttgg ggatcatgag cagcagcccc tgcgacgcgg tgaaggtcgt cgccagagac
     5701 cccgtctgca gcgcgccgtg aacggctccc gcagctcccg cttcgctctc catctccacg
     5761 acgctgggga cggtacccca gaggttcggc cgcttctgcg cggcccagtc gtcagcccac
     5821 tcccccatgt tcgaagcagg cgtgatcgga tagatggcga tcacctcgct catcgcatag
     5881 gccacgcgag ccgcggcttc gttgccgtcg atcatcgcga cgagcggttt ggctggttgc
     5941 ggcaaagtgg cgaatgacgt cggtttttcg gcgaggactg tcatgcaaag aacgatagtt
     6001 ttgccgtgac catcgactga tgacgcccgt catgccgggt ggtgatgcgc atcacttgga
     6061 tggggaaaag aaatgcacca cagagacaca gagctcacag agaaatcgat tcaactctgt
     6121 gttctctgtg tctctgtggt gcaagggttt tgtgggcact tgacctgtac gcgctttacg
     6181 agaggtagcc cgccgcgatg acgagaaccg cggcggcacc gacgatggcc tcgcgcatgc
     6241 cgatgatttt ggccgggggc gccactttct cccggaggcc gatcgcggca cgaacgagca
     6301 gcattgccat gacgatgatc gtgacggcag gggtcaggcc gcgttgccag agaatcgccg
     6361 cgccgatgac ggcggcgcca tgcagaacca tggtcgtcgt tcgccgttcg tggcgcagga
     6421 cggcgcgaac gaagaggatc gccggaagag tcagcgacgc ggcaaggagg gctaacggga
     6481 gcgcgacgga ccagctgcgg tgtgcagccg ttgcgatggc ggcggctgta gtcgcggttg
     6541 cgcatgcgcc gcagatctcc gcgacgaacg aacggccgcg ctgacgaacg tcgagtgcga
     6601 attgaatcga cgccaggagt gcgccggcca cgagcggcag gaggaagcgg gatccgctca
     6661 tcgcgaccgc gcttatcagg gcgatcgccg acattccagc gtacgcgacc gcgagccacg
     6721 cgcaggccac cgttcgtgcg tatcgtttcc ttcgccgcgc gtcttgcacg aagagtctca
     6781 gcgggtgtcg tgccaggaac gcaagaaccg ccgcggcggc gatcagcgat cctgcgagcg
     6841 agggagcgac gagaatgccg aggacgatcg gctcgaacaa catcgaccat ccgccatgtt
     6901 cggatggcag cgccagtggt ctgagcgcca ctcttcgcgg aaacgcggac gacgccgagc
     6961 cttcggtcac gacactcatg aatcgaacat agtcagaggg caaccatcga ctgatgaccc
     7021 acgtcatgag gatgggtgac ctgcatcact cgcgggacac cggctcagcg tcggatgacg
     7081 cgagtcctca aagaaatgcg cgtcgaacac agaggcgtgt tcgcgatcac ccgtcagcgg
     7141 tgactctccg catcgtccgc tatggcctca ccgcctattg tgacccgcgg gagtcattat
     7201 gcgaacgttt gtcattgcta ccgtctttct gtttgccgcc gcgttgccgg tgcgttcgca
     7261 acttaccaat accgattgcc tctcctgcca cgacaacaag gagctcacca aagacatcgg
     7321 tggcggcaag acggtcagcg taagtgtcga tccgaagaag ttcgccgcat ctgtgcacgg
     7381 gccgctcgac tgcacgaact gccacgccga cgtcaaagcc tatcctcatg atcctgctcc
     7441 gaaagctgtc gattgtgcct cctgccatcc ggacagcgtt gccgcctggg acaccagcct
     7501 gcacgcgaag gcggtgaagt cgggggtggc ccgaggagcg aagtgcgccg attgtcacgg
     7561 gccggcgcat gaggtgctgc cgtcgtccga tcccaaatcg aagactgcgc atggtgcgat
     7621 ccctgcgaca tgctcggcat gccacggaca gaagttcgtg atggcgaaag cgggactcag
     7681 caaccagtcg gcgatctcgt acgaggcgag tgtccatgga cgggccgttg ccaacggatc
     7741 ggcgaaggcc gccgtctgca ccgattgcca tgaccatcat gctgtccggc cggcgaacga
     7801 cccgctctcc ggcatcttca agttcaacgt tgcccgcacc tgcgggaagt gtcacgctac
     7861 gactgcagcg cattacgcag agagcatcca tggaaaggcg ctggcgcgcg gaaactggaa
     7921 cgctccggtg tgcaccgact gccacggcat ccacacgatc tcgcgcgccg gcgatgcggt
     7981 gaacggaccg cgttcgagtt gcgagcggtg ccacgcggga gtgcgtctca cgaaggagtt
     8041 cggcgtcgcc ggaacgcgcg tggcgacgta tcagtcgagc tatcacgggc tggcgcgcca
     8101 aatggggtcg acgacggccg ccgattgcgc gagctgccac actgcgcacg acgtcctgcc
     8161 gtcatccgac ccgcgctcgg cgatcaaccc gaggaacctg cagaagacgt gcgggaaatg
     8221 ccatcagggt gcgcagatca acttcaccca aggcaaaatc caccgcgtcg atggcgatgt
     8281 gagcgacacg ccctcacgca tcgtcacctg gatcaagcgg atctacatcg tgctgatcct
     8341 cctcacgatc ggattcatgc ttctgcacaa cgctctggtg tggtggaaga aagcagtcgc
     8401 ggcgaggaag gggcgcggcc acaccgtcga gcggatgaat ctcaaccagc ggatccagca
     8461 catggtcatg gggacgagct tcgtcatcct cgtcatcagc ggattcgcgc tggcctggcc
     8521 gaactcgtat ttcgcagctc tgttcggtcc cagcgagcag ttgcggcgga tggtccaccg
     8581 catcgcagcc gtggtcatga tcgcgatcgg gctctaccac cttgcctata tggtgttcac
     8641 ccgcgaaggg cgcaaaggtc tgcgcgacct ctggctccgt ttctccgatg gcggcgatgt
     8701 catcgccgtc ttcctctact acctcgggct ttccaaaaag aagccgcaat tcggacgatt
     8761 cagctacgcc gaaaaggtcg aatactgggc gggaatgtgg ggcacgatcg tcatggccct
     8821 gaccggattg atgatctggt actctgtcgc cgtcgcgacg tggatccccc gctggtggat
     8881 cgacgttgcc accacagtcc attactacga ggcaattctg gcaaccctcg ccattctcgt
     8941 atggcacttc taccacgtga ttttcgaccc cgatgtctat ccgatgaatt gggcatggtt
     9001 tgacgggaaa atgtctgaag aacaattcaa ggaagagcac cccaaagcag ttgattcggc
     9061 ggaaacgcat acaactacga gtgtcgaagg gaaagactaa tgacggaatc gcccaagcca
     9121 gtgcggcttt cgcgtaactt aatcagtgaa gcaggtctcg ggatcgcagt tgtagctctc
     9181 gccaacctcg gatttctgat ctatctggac gctactcgag cgaattcgaa tccctatatg
     9241 gggattctga cttggatcat cgcgccgatc attctcatct tcggcatcgt cgtctacatt
     9301 ggtggaatgc tgctcgagcg ccggcgacgc cggcgtctcg ctccgggaga gattccgcaa
     9361 tacccgcgca tcgatctcaa cgagcgccgc acccgcgtca tcattgtttc cacggcggtc
     9421 ggactcattc tctttgtgac gatgtcggtc atcggcagtt accaggcata tcgctatacc
     9481 gactccgacg ccttttgcgg cacggcttgt cacaaggtca tgactcccga atacaccgcg
     9541 taccaggcgt caccacatgc gcatgtcggc tgcgcgggtt gtcatatcgg accgggagcg
     9601 ggctggttcg tgcggtccaa gctctccggc tcgtatcagt tgtattccgt cattgcgcac
     9661 aaataccccc gtccgattcc gagccccgtc gagaaccttc gaccggctca ggaaacgtgc
     9721 gagcaatgcc attggccggc gaagttcttt ggggcgcaat tgaaggtgtt cgatcattac
     9781 gcgtacgacg agacgaacac gccgaaagaa gtgcgaatgc tcatcaagac cggcggcgga
     9841 agcgcgtccg gcgggcagac gagcggcatt cattggcaca tgaacatcgc caatgagatc
     9901 acttatatcg ccaccgacaa gcaacggcag gtcattccct gggtgcgcgt caaagaccgt
     9961 aatggaaacg ttactgagta caagtcggcc gactccaagc tgacggatgc gcagcttgcc
    10021 acggctacga agcggcgtat ggactgcgtc gactgccata atcgccccac gcacatatat
    10081 cgctcgcctg accgcgccgt cgacaccgcg atgttcacag ggaagatcga cagcacgctg
    10141 ccattcatca agcagcagag tgtcgctgtg ctggccgcgg aatatgagac gaccgatgcc
    10201 gccgtcaatg ccattgcgaa ggcgattccc gcctattacc aaaagaccta tcccgatatt
    10261 tataaagcga aacgagtgca ggtcgccggt gctacagccg ccatccagga cattttccgg
    10321 gccatccgct ttcctgagat gcgcgtcgac tggcgcacgc accccgacaa cctcgggcac
    10381 ttcgcgacga tgggctgctt ccgttgtcac gacgatcagc acgtcagcaa ggacgggaag
    10441 aaaatcagca aagactgtca gatctgccac acggttctgc ccgatgccac ggccaacgcg
    10501 agccccacat tcgctcaccc cgtcgacatc ggcgatctgc gcggcgtcaa ctgttccgac
    10561 tgccataccg gcggcggcat gtaatgaatc acaggaggta aacaatgagt cgaaaatcgg
    10621 tagcaatcat cctgagcgtt gcagtcattc tgatcgccgc gccggccgcg tttgccgcgc
    10681 ccgacggtgg tgccctttac aaggcgaagt gtgcgagctg tcacggcgcc gacggcggcg
    10741 gcatgacgcc gatggggaag gcgatgaagc tgcgcgacct gcgctctgct gacgtgcaga
    10801 agctgacgga cgccgagctg actaaggtga cggccgacgg caaggggaaa atgccggcct
    10861 acaaaggcaa ggtgtccgac gccgacatca cggcgctcgt cgcgtacatg cgcaccttga
    10921 aatagaccgc aggaacgagg agcgtcgtcg aagcggcggc gctccgttgt cttaccattc
    10981 cggcgtgctc gatccgcctg ccgaatccct ccccgacatc ggtgatgatc cgcgcttcca
    11041 tgcggcgctc gcgatgctcg cggcgctcga ctacttcgag gcgagcgacg agttcgagga
    11101 gctcttcttc gaagccgttc gcgacgaagt ggagttcgct cgcgtctttt tgcagctcgc
    11161 ggccggcggc gttcacctcg agcggaggca gtggcgcgca gccacgcagc gcctcgacga
    11221 agcagtccgc gcgatcgcac gcgtgaccaa cgaccgcggt tacgatctcg cccggatggc
    11281 ggacgagttg cggacgttga tcgagcaggt gcgccaggga tccgccttcg agtggcctcg
    11341 ggtgctgcgc cgctgacgtc gcgccagaga ggccggcgtg gccctcatca cacgctcgcg
    11401 tgacgccggg tgcggtcgcg ctcgcgaaaa ccgcgcatcg tgagaacgtg ccgcctgatg
    11461 tcatcctgat tcacccgccc agcgttttcg attttcgcga gcgggccatc gtctatgggc
    11521 cgatcagcga cgtcatcccg tcgacgcccg tctttgagat gtacccagtc ggattcctga
    11581 cgctctccgc gtatctgcgg cggcacggct acagcgtgcg catcgtgaac ctggcgttgc
    11641 ggatgatgcg cagtcgccgc ttccagcctg agcgattcct gcgcaagttt catcccaaac
    11701 tgttcggcat cgatctgcac tggctcccgc acgcacacgg cggaccggcc gtcgctgcat
    11761 tactcaagaa acttcatccc gagatcccga tcgtcttcgg gggtatctct gcaacatgtt
    11821 ttcatcggga gctgatcgaa gatcccgcag tcgacttcgt cctgcgcggc agtgtcactg
    11881 agccttcgct gcttgcactc gtgcgcgagc tcgagggtga acggcgtttc gagctggttc
    11941 ccggactaac ctggaagcag gatggcgaag tgcgcgtgaa ccccgacgcc gtgttgccca
    12001 cgtccctcga cgagtacgac ttcgacctcg gcgcgatgat ccgctccgtt gcccgtcacc
    12061 tcgacttctg gacaagtgtt ccgtttcagg cgtggtggcg gcatcccatc accgccgtat
    12121 tcaccgttcg cggctgtgcg cggcgctgca tcacctgcgg cgcgtcgaat gccgcattcc
    12181 ggcgtttcat gccgggacat cacccactcc tgcgcggtcc ggaggcgatt gccaaacagg
    12241 tgcgtcaact ggcagcgatc acgcgtacgc cgatctttct cgtcggcgat cttcgcgacg
    12301 gcggagtgag ttacgcgcat gacgtgatcg atgccctcgc acgcacgccg gtgtcgaacc
    12361 gcatcgtgtt cgagttcttc gatccccccg acgcggagct cgtggctcgg atcgacgcat
    12421 cgattcgctc atggggagcg gagctctcac ccgagagtca tgacgaatcg attcgcggcc
    12481 tcctgggcaa ggcgcgattc acgaatgccc ggatgatcca ggcgatcgaa tccgtcatgg
    12541 cctcgcgttg cgaaagcctc gaccttttct tcatgatcgg acttccgggc cagacaacag
    12601 cgaacgtgac ggagacggta gacgcgatcg aagagctctt catccggttc gaccaccgcc
    12661 tttcggcatt catcacgccg atggcgccgt tcgtcgatcc gggaagcgac ggtttcgcgg
    12721 acgcggaagc gcatggctac cgtttgcggg cgcggacgct ggccgagcat ggcgcgctgc
    12781 tggagacgag cgattgggaa tcgatgctca actatgagac cgactggatg acgcggcgtg
    12841 agatcgtcga ggctacgtac aacgccgcga agcgtctcaa cgatctgaag cggcgctacg
    12901 gcaggatcag cgaccagcgc gcgggtcgtg tcgaggcgcg tcggagcgcc gcgcatcggc
    12961 tgggcggcga aacccacgcg gtgtcggaag ggacgctgaa cgacaaatcc gagctgttcc
    13021 agccgggcgc ccttctgcgc aactttcgcg tcggcggaat cctgcgcctg ttgttcggac
    13081 gcgcgtaaat cgcgtctcgc ggtctaaatc gcggcgagtt gttcccgcgc atgtcccaac
    13141 gccagatgag actgcgcggc gtgagcattc acggtaagta ccgggcatcg gctctgttgc
    13201 acgatgcgct cggcgattga gcctcgaaga ttgtcggctg gactgcgatc tgcgctgaca
    13261 tcgatgacga tgagatccgc cgcagtcgcc agagcgaggt cgaggatttc ttccgccaac
    13321 ccacgtctcg gcgcggtctt caattggcaa cggccggcaa gctccggcgg cgcccactcg
    13381 tgcaggcgaa tcaactcctg aatgctgggc tggagctcct ggtcgtcaag accgtgaacg
    13441 agaaggatgg gtgccttatc gtcatcggcg agagcaccgg cctgccggag cgctgcggaa
    13501 caggcaggcg tgaagttgac ggcgcagaga atcctgccga ccgatgcgtt ctccgaggcg
    13561 ctggagttga cggcgatgac cgggacgggg gaagtgcgca tcaccgcttc ggtgacggag
    13621 cccgttagca ggcggcggat gccgctgcgt ccgtgcgttc ccatgaccac caggttcgcg
    13681 ccggagtcgc gcgcagcctc ggtgatggac tccatcggat gcccgacaat gaccatgatg
    13741 tcgtaaggaa ctctggcgct gatgtttctc tccgcgtgca acgcgagctg ctcgcgcgcc
    13801 gcagcgatca gcgactccgg ctccgcagtg ggtacgccga cgccgctgcc ggtgaaatcg
    13861 aacggtggaa cgaacgtgtc tgcataaacg acgaggaggt gggcaccgaa tcgttcgccg
    13921 agcgcgctgg catagcgaac ggcatgagac gccggagcgc tgaaatccgt aggcacgacg
    13981 attagtctcg gaacgagtga ctccatgctc cacctcctcg gtcaaggcgg cagtgccgcc
    14041 gcggacttag aagcccgctg tgatcaaggt aggtcggtgc ggaggcgccg cgcatgatcg
    14101 ccgtcactcg gagatgtgat ggagggtggc ggcgcctcac cgcaatgaat cggccggtag
    14161 gatgcgaaag ctctttcgtg gcgccggacg aatcgcgcgg aagtggcgtt catcgagagt
    14221 cagaatgtcg agcgtgtcgt agcgtacggc gaggacagcg atggatgcat cagccaatcc
    14281 gattccgagt ggtcggtatt tcgtgatgag gtgccgcgcg gcttcgactt cgtcttcgtc
    14341 gaaggttgcc agctcgtaag cgcggcgtcc gatctcggcg aggaagagag tctccgcatc
    14401 gacgccgccg aacttctgga tcaggtagtc ggtttcggcg agaacgaacg gcgagatgat
    14461 tagcggtgga gtagcgttca ggagagcctc tgcacactca tcgtgccagt tttgatcact
    14521 gaacagcgca gccaggatcc cgcttgtgtc gaccagaatc aacgcccgaa tctctcgagt
    14581 agcgcatcga cgttctcggc gatactcgga tctccgaggc cgcgtttcat caatggaatc
    14641 cgcggtgctg gagaagtcgt cgtcgctgtg gccgcgctga tggcgtcgcg gatcacttgc
    14701 gcttccggcc tgccgctggt gcgagccagg ttttccagtc gcttcttcag ctcgtcagga
    14761 agatagatcg tcgtcttctt cataccatac gtataccata cgttggtgct tatgggaagc
    14821 cgatgctgat cgcaaccaaa cggtcgcgtc gccgttatac ccagcgcaac actccggagg
    14881 aatcatgaga atgaagcacc gaatcgcacg cctgatggtc gtcgtagcgg tggccgccat
    14941 tggtatccag tcacccgcct atgcggtcaa aggctactcg gcgatgtctg aatccgagaa
    15001 cttccattcg gtcacgcgga ccaaagtatt caagcatagg attgcagcgc cttggaccgc
    15061 ggcgcggctg aaggttgcca tgcgcgttga cgctggcggc gctacggtgc gcttgattga
    15121 cgcgaaagga gcgacgcgct gggagaagac cttcgaaaag ggagccgtat cgatcgatca
    15181 gacgtttcca ggcaacggag actggcaggt cgagcttcgc ctcgaggatg cgactgggcg
    15241 ttatgagatt cacttgatcg cagtctgatg atcgctgtct gacctaaagc ttcactgccg
    15301 cgtcaactcc actcccgtcg cgacgaacga gacttcgtcc gcggttccgt cttcgttgcg
    15361 tttcagcgtt gcgccctctt cggcgaggtg atctgcctca gccttgctca gcgtcttgat
    15421 cgtcgtcacg ccttcggcac gggcctgcat tcctttggcg gtgaggggaa tgctgaactt
    15481 ctcatccttg aacgtgacgc gcac
//
LOCUS       DATLVW010000866         3998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_572107, whole genome shotgun sequence.
ACCESSION   DATLVW010000866 DATLVW010000000
VERSION     DATLVW010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572107"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VNN25_25410"
     CDS             complement(<1..1196)
                     /locus_tag="VNN25_25410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH94937.1"
                     /translation="MSIAEPEVAYPAHLEADVVLRNGRTLRIRPVRVDDHERMRAFFS
                     TLSPETLHARFFAVCTADQALIYAPSQVDYVHEFGVVGEVGDRIVAVAHYYSSAQHPR
                     IAEVAFAIADDQQGFGIGTLLLEKLAIAARMNHIERFEAEVLDENARMLDVFLQSGYV
                     VTRNTDRGTTHVSFAIEPTATSVARTASRAQNAAHQSMKAVFEPRSVAVVGAGRKPGQ
                     LGHEIVANLQRSGFTGDLYVVNPKANDIDGVRSYPSLRDIEGVVDLAIIVVPAAEVEH
                     VIDDCVEKSVRGAVVITAGFGETGPAGREAERRLLDKVRAAGMRMVGPNCMGIINTDP
                     AIRMHGTFAAVYPPAGNVAMSSQSGALGVAILDYARSLNIGFSTFLSVGNKADVSGND
                     LIQYWA"
     gene            complement(1599..2177)
                     /locus_tag="VNN25_25415"
     CDS             complement(1599..2177)
                     /locus_tag="VNN25_25415"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH94938.1"
                     /translation="MHQPHRQYTLDDYFSLERGVDLKLEYFNGDIFVMPSGTIGHNRI
                     NLNVLTFLHRALSGSSSEVFGVGMRVSTPSGLYTYPDASIICGPLVDGTAETIADVIA
                     LVEILSDITRNFDRGDKFEHYRSIPTLRHYLLIEPSFVHVEHRRLEADGSWSHEIAES
                     VDQVVHLSEVGVDLPVARIYEDLELATARPQG"
     gene            complement(2204..2602)
                     /locus_tag="VNN25_25420"
     CDS             complement(2204..2602)
                     /locus_tag="VNN25_25420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94939.1"
                     /translation="MSRTALKEKTITRTEAIDRLRDELMKLADDENSVCKVAAEKGIF
                     CKGFHRSSDGGLRRCYSWLDKRRPGMSRDELEDLANRWQLARQEVNQLPIACDVQQKE
                     HDSCNGWDDFTNKDLATFYFELTGAPIAVA"
     gene            2781..>3998
                     /locus_tag="VNN25_25425"
     CDS             2781..>3998
                     /locus_tag="VNN25_25425"
                     /inference="COORDINATES: protein motif:HMM:NF045502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXH94940.1"
                     /translation="MLKPVELEIELFCRGMRIDESCDTEHDGRRISRTRAGLGSGLEI
                     VIPAATKPVWVNVPVVEPFALESVFTLHESEGNYFIVDSRDGEAYEVTIPPEPAWYSR
                     NTSADVPMSRVGVLQGNYLGVYISNRCLYWSWSPSLACKFCTTGKNVGAAEESRKRVT
                     DVVEVAMAARRESGCIFTHFNTGYHYEDRADREVIHGLRQAQPFVRAVRTHVGGFIGV
                     QAVPVPRANYYEYDELIKAGTDHFSFCYEFEDPEVFARLCPGKAQTVGQHSFFEAMEY
                     TSRKLGRGRVSGEIIAGVEPIEATKRGIDRIVNAGAFPTVCIFRPTVGSEMENVPPPD
                     PEAMKEVFAYLHESCRRAGLPVGILPIEVSLVVQPEETAALVEPSLAGDLYAFKNRAL
                     RQLARPYVAWKLRP"
BASE COUNT          745 a         1226 c         1266 g          761 t
ORIGIN      
        1 tcggcccaat attgaatgag gtcgttgcct gaaacgtcgg ccttgttgcc gacggagagg
       61 aacgtcgaga agccgatgtt caacgatcgc gcgtagtcga ggatggctac gcccaacgcg
      121 ccgctctggc tcgacatcgc cacgttgcct gctggcgggt agaccgcggc aaacgtgccg
      181 tgcatccgaa tggcggggtc ggtgttgatg atgcccatgc agttcggtcc gaccatgcgc
      241 atgcccgcgg cgcgcacttt gtcgagaagg cggcgttctg cctcacggcc agccgggccg
      301 gtctcgccga agccggccgt gatcacgacg gcgccgcgca ccgacttctc cacgcaatcg
      361 tcgatgacgt gctccacctc ggcggccggc acgacgatga tggctagatc gacgacacct
      421 tcgatgtcgc gtagcgacgg ataggagcga acaccgtcga tgtcgttcgc ttttggattg
      481 acgacgtaga ggtcgccggt gaatcccgag cgctgcaggt tcgcgacgat ctcgtgtccg
      541 agctggccgg gtttccgtcc cgctccgaca acggcgaccg atcgcggctc gaaaactgcc
      601 ttcatcgact gatgcgcggc gttctgcgcg cggctggcag tgcgcgcaac cgacgttgca
      661 gtcggctcga tggcgaagga aacatgcgtc gtgccgcgat cggtattccg cgtgacgacg
      721 tatcccgact gaagaaagac gtcgagcatc cgcgcgttct catcgagcac ctcggcttcg
      781 aagcgctcga tgtgattcat gcgcgccgcg atggcgagct tttcgagcaa cagcgttccg
      841 atcccgaatc cctgctggtc atccgcaatt gcgaacgcaa cctcggcaat ccgcggatgc
      901 tgcgcagaag agtagtagtg ggcgacggcg acgatcctgt cacctacttc gccgacgacg
      961 ccaaattcat gaacgtaatc gacctgcgat ggagcgtaga tcagcgcctg atccgcggtg
     1021 cagacggcaa agaatcgcgc gtgcagagtc tcgggcgaca acgtggagaa gaacgcccgc
     1081 atccgctcgt gatcgtccac gcggaccggc cggatccgca acgtgcggcc gttgcgaaga
     1141 acgacatcgg cttcaaggtg cgcgggatag gcaacttcag gttcagcaat gctcatggag
     1201 aacacagtag cgggaggcgt ttcagagggc aggcaaacgg gtcacgtgag gaggtgatgg
     1261 atgtcacgcg agggcgatcg cgctgcttct tgacgtgtcc caaaaaggcg cgctcttatc
     1321 ctcatatcgc gtccaccatt cgtgtcatcc tgagaccgcc ggagccgctg ccccgcgccg
     1381 cgtctgcaac cggctggaaa cagcggcgga ggacggcgaa ggatctctaa gtacgatgca
     1441 catgccacat gcctcgtaac tagagatcct tcgccgtcct ccgccgccac atcacgctgc
     1501 gcgaattgtt cgagcgcgag cggcggcttc ggctgtctca ggatgacacg aatgatgtgc
     1561 agtgcaaggg tggcgaaaag cgcgctgcac tgaacgcttt acccctgagg ccgcgccgtg
     1621 gctaattcga ggtcctcgta gattcgcgcc acaggcagat cgacgcctac ctcggagaga
     1681 tgcacgacct gatcgactga ctcggcgatc tcgtgagacc acgagccatc agcttcaaga
     1741 cgccggtgct ccacgtgcac gaacgacggc tcgatcagca ggtagtgccg cagcgttggg
     1801 atcgaacggt aatgctcgaa cttgtcgccg cggtcgaagt tgcgcgtgat gtcggagaga
     1861 atctcgacga gcgcgatgac gtcggcgatc gtctccgctg ttccgtcgac tagcgggccg
     1921 caaatgatgg atgcgtcggg gtaggtgtag agaccggacg gcgtgctcac gcgcatgcca
     1981 accccgaaca cttcgctgga ggagccggag agtgcgcgat ggaggaaggt cagaacattc
     2041 aggttgattc tgttgtggcc gattgtgccg ctcggcatga cgaagatgtc gccattgaag
     2101 tactcgagct tgagatcgac acctcgctcc agcgagaagt agtcgtcgag cgtgtattgc
     2161 ctgtgcggct gatgcactct ctgattctac ccttgcgccc ccctcacgcc accgcgatcg
     2221 gcgcacccgt cagctcgaag tagaacgtcg ccagatcctt attcgtgaag tcgtcccagc
     2281 cgttgcagga gtcgtgttct ttctgctgga catcgcaggc gatcggcagt tggttgacct
     2341 cctgcctcgc gagctgccag cgattggcga ggtcttccaa ctcgtcgcgc gacatgccgg
     2401 ggcggcgctt gtcgagccat gagtagcagc ggcgcagtcc gccgtcgctg ctgcggtgga
     2461 agcccttgca gaagatccct ttttccgcgg cgaccttgca aacggagttt tcatcgtccg
     2521 cgagcttcat cagctcgtcg cgcaggcgat cgatcgcttc ggtccgtgtg atggtttttt
     2581 ctttcagtgc tgtgcgggac attctggcct ccaatgctgc atgtacagtg cgccgatgcc
     2641 ggaaaagccg ccgtaggctt gctcacaccg ccatgtgttg cgcgtcacac gacgctgccg
     2701 gtgccccggc atgacagccg tcacatcccc ttgtgacatc gatcatcgga ccgagctgta
     2761 ggtcagtttc taatcgccgc atgctaaaac cggtcgagct ggagattgag ttgttctgcc
     2821 gcgggatgcg catcgacgaa tcgtgcgata cggagcacga tggacgccgc atcagccgca
     2881 cacgcgcggg ccttgggtcg gggcttgaga tcgtcattcc ggcggcaaca aagccggtgt
     2941 gggtcaacgt ccctgtcgtc gagccgttcg cgctggagtc cgtcttcacg cttcacgaaa
     3001 gcgagggcaa ctacttcatc gtcgactcgc gcgatggcga ggcgtacgaa gtcacgatcc
     3061 cgccggagcc tgcctggtac tcgcgaaaca cgagcgccga cgttccgatg tcccgggtcg
     3121 gcgtcctcca gggcaactac ctcggcgtct acatctccaa ccgctgcctc tactggagct
     3181 ggtcgccgtc gctggcctgc aaattctgca cgaccggcaa gaacgtcgga gccgccgagg
     3241 agtcgcgcaa gcgcgtgacg gacgtggtgg aagtggccat ggccgcgcgg cgggagtccg
     3301 gctgcatctt cacgcacttc aataccggct atcactatga agaccgcgcc gaccgcgaag
     3361 tcattcacgg tttgcgtcag gcgcagccct tcgtgcgcgc ggtgcgcacg catgtcggcg
     3421 gattcatcgg cgttcaggcc gtgccggtgc ctcgggcgaa ttactacgaa tacgatgagc
     3481 tgatcaaagc cggcaccgat cacttctcgt tctgctacga gttcgaagat ccggaagtct
     3541 tcgcacgtct ctgtcccggc aaagcgcaaa cggtcggcca gcattcgttc ttcgaagcga
     3601 tggagtacac gtcgcgcaag ctgggacgcg ggcgcgtatc gggcgagatc atcgccggcg
     3661 tcgagccgat cgaagcaacg aagcgcggca ttgaccgcat cgtgaacgcc ggcgcgtttc
     3721 cgaccgtctg tatcttccgc ccgacagtcg gcagcgagat ggagaacgtt ccgccaccgg
     3781 atcccgaggc gatgaaggaa gtcttcgcat accttcacga gagctgccgc cgcgcgggat
     3841 tgccggtcgg cattctcccg attgaagtat cgctggtcgt gcagcccgag gagacggcgg
     3901 ctctcgtcga gccgagcctg gccggcgatc tctacgcctt caagaaccgc gcgctgcggc
     3961 aactggcgcg gccgtatgtg gcgtggaagc tgcgtccg
//
LOCUS       DATLVW010000867         7806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_119395, whole genome shotgun sequence.
ACCESSION   DATLVW010000867 DATLVW010000000
VERSION     DATLVW010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119395"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VNN25_25430"
     CDS             complement(<1..268)
                     /locus_tag="VNN25_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94941.1"
                     /translation="MAVKSDRRELKPEAELRSYMGRLDPKNQKLIRSVRAGVCKRFPT
                     ANELLYDYNHFFVIGYSPTDRGIDSIVAIAARVTGVFLYFSQGPR"
     gene            767..1090
                     /locus_tag="VNN25_25435"
     CDS             767..1090
                     /locus_tag="VNN25_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94942.1"
                     /translation="MTLAELFAQLTGKTVIQKEEHCQGLIGCEIEIVGRIGEIYSNLI
                     GLRGIVDLPSDATIDVFHDGKLRQQLLILAQHDAVKLAARLRYVDFGRRTMMVFDLSS
                     IVKQA"
     gene            complement(1488..2702)
                     /locus_tag="VNN25_25440"
     CDS             complement(1488..2702)
                     /locus_tag="VNN25_25440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94943.1"
                     /translation="MAKGSRLQWWRLQWWTPLRFLALVLIASAIAFVLLTITKLDMKS
                     AAGMNLKSTTGTTWSWFWLFALLPGTVGVANYIVTLRQRSAGDDGSESGPEEAESHHR
                     PSFSEALAAALVLSGIFLVVAKAAAPELNLDGLVYAGYGAYVSTLWSMLVRLNVSALS
                     PRFLINSALKTSIAMLLGFVASGTAGLFKSATPALCFLIGFCLGWALKALKKSAMVTF
                     GVTQTTAADLSVRLLEGVDDGAVDVLEELGITSIQHLATMNAAEVCGRSQYPRDRVLD
                     WIDQSILAIHANGRINDFRALGIRSAYALVTIADYACGKNKEEEPLHGLALHRLNEAS
                     NRLGQSQEALQITAECIRRDPSYIKLDATYPNRHDPTCPPIEEKKPDQKPLPGSMLPI
                     KQPVDTRVVGVG"
     gene            complement(2980..6777)
                     /locus_tag="VNN25_25445"
     CDS             complement(2980..6777)
                     /locus_tag="VNN25_25445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH94944.1"
                     /translation="MEVHPPHLAHARSALAKWLRGGRTRASHESLRTTVAIVRAQQEA
                     TLDGILVVDDKGAILSYNRRFLEMWGIPEEAANAADDNELLGFASEKVKDWDEFIELV
                     NHLYTHPDEVRSDDIVNLKDGRVLSRASVPIISGRRIRGRAWYFHDVTESVRNEAMQS
                     ALFRIATLSRDARNLQEFYGAVHHIVAGLMEATNFYIAEYDAVRDIVTFPYFVDQFDD
                     VAPEGRPVGRGLTAYVLRTGKPLLATPEEFEELRKSGEVESIGAPSVDWLGVPLATGD
                     RTWGVLGAQTYDESVRYTERDRDLLVFVAQHVASAVEQKRQEDALRESERRYRQMFEN
                     NRAVQVLLDPDTGAIVDANVAAVEFYGWDIDTLRSMHIWQINTLDEEAIRAEMTNATR
                     QQRSYFVFRHRLANGDLRDVEVHSGPIVSGGRTLLYSIIHDVTERKIAEEALLLSEQK
                     YRNIVDYASVGIYRSTPDGRILMANPELARILGYDSIEDLQGRNLATDVYLDERERAN
                     ALERFAANGFTIEHELLWRKKDGTPIWVQLNAHAIDLQAGAEVFEGFVFDITERKLAD
                     QQVSAANAQRKAVLDAATRVSIIATNPSGIITVFNSGAERMLGYSAIDVIGVRNITEL
                     HLESELEQHARRLAFELGEKLNGFEVLVRRAAREGLEEREWTYVREDREPLTVLLSVT
                     ALRDEHGHLTGYLHVGTDITERKVAEETLRQQSAAMTASMDGIGILDSRLDFTYVNDA
                     LAKLFGYPDPQQLTGRSICDLYEPPEQVRVITTVVPLVQQRGRWRGEASGVRNDGTTF
                     PLEISLTAIDGGGMVCVVRDITERTYAEEQIKHLAYHDALTTLPNRLLFKDRVTVSIS
                     HAQRHNTRVAVLFLDLDRFKVINDSLGHSIGDQLLQSVAARVQSCVRDSDTVARLGGD
                     EFTLLLPDLVHAEDAAVVAQKILEAVRYPFHIEGREFYITTSIGISVFPEDGPDAETL
                     IKSADTSMYQAKEQGRDNYQLFNASVNSRALQRIALEHGLRRSLSHNELAVHYQPIFD
                     LRTNRITGIEALLRWTDPVMGNIPPATFIPIAEAIGIMIPIGTWALREACIQAKKWHD
                     AGFNTLSLAVNLSVVQLQQIDLTEIVRDILTETGLPPRLLELEITESGAMLSPELSIR
                     TLYDLKKIGIRISLDDFGTGHSSLAYLKRFPIDTLKIDQSFIHDITRDPDTAAIVTAI
                     IAMGHSLRLKVIAEGVEFTDQAVFLKRHNCDQMQGYLINVPMPAEELELILAAGEVKA
                     DVT"
     gene            complement(6823..7494)
                     /locus_tag="VNN25_25450"
     CDS             complement(6823..7494)
                     /locus_tag="VNN25_25450"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HXH94945.1"
                     /translation="MGRLHLGTILGTTITLDFSFLLLIAFFVILNMQSVGMQNALLWA
                     PVILISILFHEFGHATMIGVLGFGSSAIVLEGMGGSTYNERRARPWQDLLISAAGPAS
                     SFLLAWLTSLLIANVGYVSHDRFFAALLPVMAYANLVWGIFNLLPIGPLDGNGILRNF
                     FRLFLQERLAFTISIWISMLVGLAVFIYCLVGRQIFIAILVAFYVRSSWMQWEFFRSH
                     NRTDD"
BASE COUNT         1465 a         2245 c         2547 g         1549 t
ORIGIN      
        1 accgcggtcc ctgactgaag tacagaaaca ccccggtgac acgggcggcg atcgccacga
       61 tggaatcgat ccctcggtcc gtcggcgaat agccgataac gaagaagtgg ttgtagtcgt
      121 acagcaactc gtttgcggtc gggaatcgct tgcatacacc agcccggacc gatcgaatga
      181 gcttctgatt cttcggatca agcctgccca tgtaggaccg cagctcagcc tcgggcttaa
      241 gttctcttct atcgcttttc actgccatga catcaggcac tccacgaaga ccacttgacg
      301 caaaatcacg ccgcccaacg gggctcggtg cggcgtcgat cgtaacatgc gctatcgcat
      361 gggttggctc actgctgctg tggtcatggc gcggtttttc gtcgggttgg tgacactttg
      421 atcgcatcac cgtcttcatg ttccggatcg agtgtcggtt cagactcttg gagctatcac
      481 tccggtccaa ccaccgaggc gggacgcccc gatggccgcg ggcgagacgc ccacagtcct
      541 cttttcggag tgcggtcgtc tcgaccgcgg gcactcaggc gtcctcgcct gagtgctgtt
      601 gggctgcggc cgaaatccgt cgaccgctcc cgcctgcgag aaatacggca gctgattttc
      661 taccgcgcag ccatcaacct ggatgtatta ttcaagactg atatcctttc aatgttccag
      721 cgccgaggtg taaccatcga aacggccgag gggctgagga ctcttgatga cgctcgccga
      781 gctgtttgcg caacttaccg gcaagaccgt gattcagaaa gaggaacact gtcaaggtct
      841 gatcggctgc gagatcgaaa tagtaggccg tatcggagag atatatagca atctgatagg
      901 tctccgcggc atcgttgatc taccatcaga cgcgacgatc gatgtattcc acgacgggaa
      961 attgcgccaa cagctgctaa ttctggccca acatgatgcc gtcaagctag cggctcgact
     1021 tcggtatgtt gattttggcc gtcgcaccat gatggtgttt gacctctcat caatcgttaa
     1081 gcaggcatga ttcgtgggac gcgttcaggt cagcgtatga tcgccgggcg gaacgggatt
     1141 ggctacagtg gccgtttgct caggggtcag gtccaaagtc tgaagcgaca gcgccagaga
     1201 acccgcgagc caagcgcatc attcggtact ttagactctg gacctgaccc caagtttgtc
     1261 cgtactccca ttttcgggca acacggatcc aagctatcac tatggtccaa ccaccgaggc
     1321 gggacgcccc gatggccgcg ggcgagacgc ccacactccg tcgtgaggaa gtgcggtcgt
     1381 ctcgaccgcg ggcactaagg cgtcctcgcc tgagtgctgt cgggcggcgg ccgaaacccg
     1441 tcgaccggtc tcgcttgaga agagtacagc cgcggtctct agcggggcta ccctacgccg
     1501 acgactcttg tgtcaaccgg ctgcttgatc ggcagcatcg agcccggcaa tggcttctgg
     1561 tccggcttct tctcctcgat cggcggacag gtgggatcat gccggtttgg ataggtagcg
     1621 tccagcttaa tataagacgg atcgcgtcgg atgcactcgg cggtaatctg caacgcttcc
     1681 tgagattggc cgaggcggtt gctggcttca tttagtcgat gcaacgctag cccgtgcaac
     1741 ggttcctctt ccttgttctt gccgcaggca tagtcggcga tcgttacgag tgcatacgcg
     1801 gatcggattc caagggcacg gaagtcgttg atgcgcccgt tcgcgtgtat ggcgaggatc
     1861 gactgatcga tccaatccag cacgcggtct cgaggatact gcgatcgtcc gcaaacctcg
     1921 gcggcgttca tcgtagcaag gtgttggatc gatgtgatgc caagctcctc gaggacatcc
     1981 acggccccgt catcaacgcc ctcgaggagg cgcacggata gatcggccgc ggtcgtctgc
     2041 gtcacaccaa acgtcaccat agcgctcttc ttcaatgctt ttagcgccca tccgaggcaa
     2101 aagccgatca ggaagcaaag cgccggagta gcgctcttga agagcccggc ggtgcccgac
     2161 gccacgaagc caagcagcat agcgatcgat gtctttaggg cgctgttgat cagaaagcga
     2221 ggcgaaagcg cgctaacgtt gagacggacc agcatagacc acagagtgct gacgtaggcg
     2281 ccgtatccgg cgtagacgag accgtccagg ttcaattctg gcgcggcggc tttcgcgacg
     2341 acgagaaaga tccccgagag cacaagggcg gccgcgagcg cctcgctaaa gctcggccta
     2401 tgatgcgact ccgcttcctc gggaccgctt tcgcttccgt catccccagc actgcgctgt
     2461 cgaagcgtga cgatgtagtt cgccacgcca acagttccgg gcaacagcgc aaaaagccag
     2521 aaccaggacc atgtcgtccc agtcgtgctc ttcaggttca tcccagccgc gcttttcatg
     2581 tccagcttgg tgatcgtaag caagacgaac gcaatcgcgc tggcgatcag gacaagcgcg
     2641 aggaacctca agggcgtcca ccactgcaac ctccaccact gcaaccggct gcctttcgcc
     2701 atcgcatcct cctctctaca gggagggaca acggcgagtt tttccgtacc gcggcgtagc
     2761 agaaaccaga ggtagttgcg gtcactttcc gaaatccgcc attgacacgt ttcgagcctt
     2821 cgacttccgg ccgctgctac cacttgtaat gtacttggtc tcgcagatga tcgcgcgcgg
     2881 cggcgagacg ccgccgggcc agccgccggg acggcggcgt tccgccgcac caacgcgcta
     2941 gcagagttgc atggcgcaac ccgcgctgcc gcgcatcccc tacgtcacat ccgccttcac
     3001 ttctcccgcg gccaggatca actccagctc ctcggcaggc atcggcacgt tgattaggta
     3061 gccctgcatc tggtcgcagt tgtggcgttt caggaagacg gcctggtcgg tgaactcgac
     3121 gccctcggcg atcactttca ggcggaggct gtggcccatg gcgatgatgg cggtgacgat
     3181 ggcggcggtg tcggggtctc tggtgatgtc gtggatgaag gattggtcga tcttgagcgt
     3241 gtcgatgggg aagcgtttca ggtacgccag cgacgagtgg ccggtgccga agtcgtcgag
     3301 ggagatgcgg atgccgattt tcttgaggtc atagagtgtg cggatgctca attcggggct
     3361 gagcatcgcg ccggactcgg tgatctccag ttcgagcagg cgggggggca ggccggtctc
     3421 ggtgaggatg tcgcgcacga tttctgtcag atcgatctgc tgcaattgca ctacagaaag
     3481 gttgacggcg agcgacaacg tattgaagcc ggcgtcgtgc catttcttcg cctggatgca
     3541 cgcctcgcgc aacgcccacg ttccgatcgg aatcatgatg ccgatcgctt ctgcgatcgg
     3601 gatgaaggtc gccggcggaa tgttgcccat tacggggtcg gtccagcgca ggagcgcttc
     3661 aatgccggtg atgcgattcg tgcgcaggtc gaaaatgggc tggtaatgta cggccagctc
     3721 attgtgcgat agcgatcggc gcaatccgtg ctcgagcgca attcgttgca gtgcccgtga
     3781 attcaccgag gcattgaaaa gctggtaatt gtcgcggccc tgttctttgg cctggtacat
     3841 cgacgtatcg gcgctcttga tcagagtctc ggcgtcggga ccgtcttcgg ggaaaacgct
     3901 gatgccgatc gacgtcgtga tgtagaactc gcgcccttcg atgtggaacg ggtagcggac
     3961 ggcttctaga atcttttgcg cgacgactgc ggcatcctcg gcgtgcacga ggtcggggag
     4021 gaggagcgtg aactcgtcgc cgccgaggcg ggctacggtg tcgctgtcgc ggacgcacga
     4081 ctgcacgcgc gcggcgacgg actgcaggag ctggtcgccg atgctgtggc cgagggaatc
     4141 gttgatgact ttgaagcgat cgaggtcgag gaagaggacg gccacgcggg tgttgtggcg
     4201 ttgcgcgtgc gagatcgaga cggtgacgcg gtctttgaac agcaaacggt ttggcagcgt
     4261 cgtcagtgca tcgtgatacg ccaggtgctt gatctgctcc tcggcgtacg tgcgttcggt
     4321 gatgtcgcgg acgacgcaaa ccatcccgcc gccgtcgatc gccgtcaggg agatttccag
     4381 gggaaaggtt gtgccgtcgt tgcggacgcc gctggcctcg ccgcgccagc ggccgcgttg
     4441 ctgcacgagc gggacgacgg tcgtgatgac gcgcacctgc tcgggcggtt cgtagaggtc
     4501 gcagatcgag cggccggtga gctgttgcgg atcgggatag ccgaacagct tcgccagcgc
     4561 gtcgttgacg taggtgaagt cgagacggct gtcgaggatg ccgatgccgt ccatcgacgc
     4621 ggtcatcgct gccgattgct ggcgcagcgt ctcctcggcg accttgcgtt cggtgatgtc
     4681 ggtgccgacg tgcaggtagc cggtgaggtg tccgtgctcg tcgcgcagtg cggtgacgga
     4741 gaggaggacg gtgagcggct cgcggtcttc gcggacgtag gtccactcac gctcttcgag
     4801 cccctcgcgc gcggcgcggc gcacgagcac ttcgaagccg ttgagttttt cgccgagctc
     4861 gaatgcaagg cgccgggcgt gttgctcgag ctcggattcg aggtgaagct cggtgatgtt
     4921 gcggacgccg atgacgtcga tcgcgctgta gccgagcatt cgttccgcgc cggagttgaa
     4981 gacggtgatg atgccggatg ggttggtggc gatgatggag acgcgtgtgg cggcgtcgag
     5041 cacggccttt cgttgtgcgt tcgcggcgga aacctgctgg tcggcgagct tgcgctcggt
     5101 gatgtcgaag acgaagcctt cgaagacttc ggcgcctgcc tgcaggtcga tggcgtgcgc
     5161 attgagctgc acccagatgg gcgtgccgtc cttcttgcgc cacagcaatt cgtgctcgat
     5221 cgtgaatccg ttcgcggcga agcgctccag cgcgttcgcg cgctcgcgtt catcgagata
     5281 gacgtcggtg gcgagattgc ggccctgcag gtcttcgatg gagtcgtacc cgaggatgcg
     5341 cgccagctcc ggattggcca tcagaatcct gccgtccggc gtggagcggt agatgccgac
     5401 cgaggcgtag tcgacgatgt tgcggtattt ctgctcggac agcagcagcg cctcctcggc
     5461 gatcttcctc tccgtgacgt cgtggatgat cgagtagagc agcgtgcgcc cgccggagac
     5521 gatcgggccc gaatgaactt cgacgtcgcg cagatcaccg ttggcgagcc ggtgacggaa
     5581 gacgaaatag ctgcgctgct gccgggtggc attcgtcatc tcggcgcgga tagcctcctc
     5641 atcgagcgtg ttgatctgcc agatgtgcat cgagcgcagt gtgtcgatgt cccatccgta
     5701 aaactcgacg gcagcgacgt tggcatccac gatcgcaccg gtatcggggt cgagcagaac
     5761 ctgcacggcg cggttgttct cgaacatctg ccggtagcgc cgctcgctct cgcgtaaggc
     5821 gtcttcctgg cgcttctgtt cgacggccga ggcaacgtgc tgggccacga agacgaggag
     5881 atcgcggtcg cgctccgtgt agcgaacgct ctcgtcgtag gtctgcgcgc cgagaacgcc
     5941 ccaggtgcga tcgccggtgg ccagaggtac gccgagccaa tcgacggagg gggcgccgat
     6001 cgattcgact tcaccgctct tcctgagctc ttcaaattcc tcgggcgtgg caagcagcgg
     6061 ctttccggtg cggagtacgt acgcagtcaa cccgcgtccg accggacggc cttcgggcgc
     6121 aacgtcgtcg aattggtcga caaagtaggg gaaggtgacg atgtcccgga cggcgtcgta
     6181 ctcggcgatg taaaagttcg tcgcttccat gaggccggcc acgatgtggt gcacggcgcc
     6241 gtagaactcc tggaggttgc gcgcgtcgcg gctgagcgtg gcgatgcgga agagcgccga
     6301 ctgcatggcc tcgttgcgca cgctttcggt cacgtcgtgg aagtaccagg cgcggcctcg
     6361 aatgcgccgt cccgagatga tcggcaccga ggcgcgggag aggacacggc cgtccttgag
     6421 attgacgatg tcgtcgctcc gcacttcgtc gggatgcgtg tagagatggt tgacgagctc
     6481 gatgaactcg tcccagtcct tcaccttctc cgaggcgaat ccgaggagct cgttgtcatc
     6541 agcagcgttc gcggcctcct cggggatgcc ccacatctcc agaaagcggc ggttgtagga
     6601 gaggatcgcg cccttgtcat cgacgacgag gatgccatcg agtgtggcct cctgctgggc
     6661 acgcacgatg gccacggtcg tccggagcga ttcgtgggag gcgcgggtcc ggccgccgcg
     6721 aagccacttg gcgagcgcgc ttcgagcgtg cgcgagatga gggggatgta cttccatacg
     6781 ggttggcagg tctattctag gtgatgttcc gtccgtcttg tttcaatcgt ccgtccgatt
     6841 gtgggatcgg aagaactccc actgcatcca actcgaccgg acgtaaaacg cgacgagtat
     6901 ggcaatgaag atctgccgcc ccaccagaca gtagatgaag acggccagcc ccaccagcat
     6961 cgagatccag atcgagatgg tgaaggcgag acgctcctgg agaaacagcc gaaaaaagtt
     7021 gcgaaggatg ccgttgccgt cgagcggccc gatcggaagc aggttgaata taccccagac
     7081 caaattcgcg taggccatca ccggcaggag ggcggcgaag aagcggtcat gggagacgta
     7141 gccaacgttg gcgatgagca gactggtgag ccaggcaagg aggaagctcg aggccgggcc
     7201 ggcggcgctg atgaggaggt cctgccacgg tctggcgcgg cgctcgttgt aggtggagcc
     7261 tcccattcct tcgagaacga tcgcgctcga tccgaagccg agaacaccga tcatggtggc
     7321 gtgaccgaac tcgtggaaaa ggatgctgat caggatcacc ggcgcccaga gcaacgcgtt
     7381 ctgcatcccc accgactgca tgttgagaat gacgaagaaa gcgatgagca gcaggaagct
     7441 gaaatcgagc gtgatggtgg taccgagaat cgtaccgagg tggaggcgac ccatgaggtg
     7501 ggggagtata cgttggaggc gagtggaggt tgaccgtccg aggcacgcac cgcaacgact
     7561 tcgcccatgc gacgggcaca cgacgacagc ctcttcggtt acgccgggac tcgcggtgat
     7621 tcccgggatc ggaccggaag cgcgagggga gcgccgttct cgctcattcc gtgggtgaac
     7681 tgttatcgcg ttgcttgctg ggcagatgga ccgtttaact cgcgatcgag acgctccgag
     7741 agcttctgca tgctgtgggc ggttacgccg aatccctcga atagatgtac tttgatttcg
     7801 acaatg
//
LOCUS       DATLVW010000868        10470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_614209, whole genome shotgun sequence.
ACCESSION   DATLVW010000868 DATLVW010000000
VERSION     DATLVW010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614209"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..167
                     /gene="yidD"
                     /locus_tag="VNN25_25455"
     CDS             21..167
                     /gene="yidD"
                     /locus_tag="VNN25_25455"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXH94946.1"
                     /translation="MQCRFTPTCSWYGHESIRKHGFLIGGAKTFWRIARCGPWTKLGT
                     YDPP"
     gene            276..656
                     /locus_tag="VNN25_25460"
     CDS             276..656
                     /locus_tag="VNN25_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94947.1"
                     /translation="MAAKKKTSKKKASKKATAKKKAAKKAAPKRAVKKAPAKKKKAAA
                     KKVAAKKAPARKAAKKAVAKRAPAKKKPAKKSAPKPAAPRPSESLLPQTTTAPAGSAA
                     VAPDMAGDQEGSDSENERGAFDHF"
     gene            971..1351
                     /locus_tag="VNN25_25465"
     CDS             971..1351
                     /locus_tag="VNN25_25465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94948.1"
                     /translation="MLQGRKNTSEVEIFILEVEIFILKVEVFIFDAEVFIFEAEIFIF
                     EAEVSIFEAEVFIFEAEVSIFGGRGIYLRGSRYLPSRSRYSPSRPKVFISEGEVFTSD
                     ANLCISEVEVRSIRSWVTARWTPR"
     gene            complement(1461..2108)
                     /gene="nth"
                     /locus_tag="VNN25_25470"
     CDS             complement(1461..2108)
                     /gene="nth"
                     /locus_tag="VNN25_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXH94949.1"
                     /translation="MATKKQERANDIVIRLKRMYPKAKCALDFTNAFELLIATILSAQ
                     STDARVNIVTKSLFRKYPGPKEFAEASQVEMERDVKQTGFFRNKAKAVIAASKAIVER
                     HNGEVPRTMDELTALPGVGRKTANVVLSNAFKIAIGIVVDTHVTRVSARLGLTATDDA
                     VKIEQDLMALIPQKEWINFSHRLIAHGRTICIARKPKCAQCALNELCPSAEAPPE"
     gene            2165..3829
                     /gene="hutU"
                     /locus_tag="VNN25_25475"
     CDS             2165..3829
                     /gene="hutU"
                     /locus_tag="VNN25_25475"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXH94950.1"
                     /translation="MTTTIEEARVIRAPRGTTLSCLGWQQEAALRMLMNNLDPEVAER
                     PEELIVYGGIGKAARNWECFDAIVATLKRLKNDETLLVQSGKPVGVFRTHENAPRVLI
                     ANSNLVPHWATWDEFRRLDALGLTMYGQMTAGSWIYIGTQGILQGTFECFASVAKTRF
                     DNTLRGKLVVTAGLGGMGGAQPLAATMNEATALVAEVDETRIQRRLDKRYLDEKAPSL
                     RAAIERAIAARDAGEAVSIGVVANAVELLEALLERNIVPDILTDQTSAHDELNGYVPA
                     GLSYDAALAMRDSSPDAYIELSYDTMARHMRAMLELQRRGAITFDYGNNLRGQALKAG
                     VKDAFDVGGFVPLFIRPLFCEGRGPFRWAALSGDPNDLFATDEALLELFPHDAALHRW
                     IRMAQKRVEFQGLPARICWLGYGERDKAGLLFNDLVRTGKVKAPIVIGRDHLDAGSVA
                     SPNRETEAMKDGSDAIADWPILNALLNTAAGATWVSFHHGGGVGIGYSLHAGMVVLAD
                     GTDAAAERLQRVLTTDPGTGVMRHADAGYEIAIEAAKERGVDIPML"
     gene            complement(3929..5764)
                     /gene="glmS"
                     /locus_tag="VNN25_25480"
     CDS             complement(3929..5764)
                     /gene="glmS"
                     /locus_tag="VNN25_25480"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXH94951.1"
                     /translation="MCGIIGYAGTRDVVPVLIGGLKKLEYRGYDSAGIAVVDGSRVDV
                     VRAEGKLSNLEAKLSEKPLSGTFGMGHTRWATHGKPNENNAHPHRDCTGAIVVIHNGI
                     IENFLPLKQRLQKAGHEFKTETDTEVVAHLIEENMKDGTKFVEAATKMLKELDGHYAL
                     VMIDGNEPGTIIAAKHGPPLVVGLGDNENIVASDVAPLLAYTRDIIYLEDGEYAIVDQ
                     KNVSVFNEKDEKVERAPKRILWDAVMAEKEGYRHYMLKEIHEQPRAVRDTFTGRMFEE
                     SGEIFFNDLQFTPDEWAKIRKVHVIACGTSWHAALVGKFLLEDAARIPIEVDYGSEYR
                     YRNPIIDESTLVIGVTQSGETADTIAGMQEAKKRGAKLITICNVIGAAATRMSDGVIY
                     TNAGPEIGVASTKAFTTQLTAFYLLSLYIRQLRGGDLDDRRFAMHELSVIPHKIEDTL
                     AKQEKHIEALAHKYSKSQDFLFLGRGVHYPIALEGALKLKEISYIHAEGYPAGEMKHG
                     PIALIDENLPVVAIATHSPVYDKVVSNLQEVKARDGKLIVICDEGDDAVKALADDVIE
                     IPWTIEPLQPILTVVPTQLLAYYIALRRGCDVDQPRNLAKSVTVE"
     gene            5864..7015
                     /locus_tag="VNN25_25485"
     CDS             5864..7015
                     /locus_tag="VNN25_25485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94952.1"
                     /translation="MLPKKLVVLLAVAVLACGKRGDPFPPVPVIPKATTDLLVAQRGP
                     KVLLSWSYPSLTTSGKTMTGIKRIVIWRSVEELPVATSAPPSPIEQPVQLFAKVPPLG
                     PNQFLKLRTKVDSIESANLKAATTGARLTYEDAPPLHAANGRPVRITYSVVTQGPVVS
                     GDLSNLPTIVPLDVPVAPTALAATAKKEGVVLTWTAPETSISGAKNPPVAGYNVYRFP
                     HGQPADEFAAPANPSPVTATTYTDQPPYGTHDYFVTAVAASGTTRIESEPSAIAGAEF
                     KDLVPPAPPGGLTALVETGAVRLVWDPVDAPDLAGYRIYRTEGTGIEQLRVAGTIPLN
                     PVQLLTATNYRDTGVNPGISYYYEVVSVDKSGNESARSKTDWVLVPKTP"
     gene            complement(7201..7488)
                     /locus_tag="VNN25_25490"
     CDS             complement(7201..7488)
                     /locus_tag="VNN25_25490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94953.1"
                     /translation="MDSTTEVRSPIFERRRLRRIRMIVELTLNLIGSDMTVSHREARC
                     LVRCARKAILDLHPGFEQRYERIVRPYFERLLASRWPHEQALDDDSCDVVN"
     gene            complement(7612..8250)
                     /locus_tag="VNN25_25495"
     CDS             complement(7612..8250)
                     /locus_tag="VNN25_25495"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HXH94954.1"
                     /translation="MGRARELKLRADSMLEEVFRSLFEEIDTEKIRRDVDALKASAPD
                     FHSDDHARLLVRRTALRCAAAGAVSGLPSGLIAIGTLGAELAFLVFQQFRLILGIATV
                     YGHEPTQRERFAEAMSCLAYASGVGIGKQGIAVMLESATMDGGMIAEKLGANFFRDRL
                     TKAIPFVGALSGGALNYVSVRAVGRAAIKFYESQIDATIADEIWAEGDREHA"
     gene            complement(8257..8661)
                     /locus_tag="VNN25_25500"
     CDS             complement(8257..8661)
                     /locus_tag="VNN25_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HXH94955.1"
                     /translation="MPIYEYQCTKCNDRTEVIQKFSDPPYAVCDKCGGDVKKLMSAPA
                     IQFKGSGFYKTDYASASSKESKSDSKSESKSDGKSEGSGESKSSDSKSDSKSESKSDS
                     KSESKSESKSETKSESRSETKSESKAESKPSA"
     gene            complement(8871..9464)
                     /locus_tag="VNN25_25505"
     CDS             complement(8871..9464)
                     /locus_tag="VNN25_25505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94956.1"
                     /translation="MRRALILISLAAVACGGGNAIDNSSEWLRVLHHKQAAQAPNAPA
                     QAKQVYADTLGTFVRNHPTHSRAREVYQHIQLDFARELSSLGRYQDAIRIYRAILTHD
                     PQNIAAMQGLSSAANHLAVSREKLLALQKGMSQRDVADLLGKPIPGWQIRTERPDTTI
                     EAWYYRRIDGGIAGVYFRDGALFAAEENSQAKVAPLM"
     gene            9548..>10470
                     /locus_tag="VNN25_25510"
     CDS             9548..>10470
                     /locus_tag="VNN25_25510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94957.1"
                     /translation="MAVRGDEVAGSIEAHVRDAMEKLVGEIRSSIEDVREVVDSQLRA
                     ALQSVQADVNAVSFLPHIQKSIGDLEESITSAQPAAAVPAPSGGDASLLKRSIQAVER
                     GKSQVDILNALLEQCLAFGSRAAMLILRNETFSGWKGVGFTAAGGNDEMVKRFNAAPG
                     LIPELDRVLRSEEVVLWDGANLSTRLGVPASSRAVLVPMVIKDKVAAAVYVDATEDDA
                     AKLDASSIELLVFATGLLIDTLTIRKKIPSPSLNDSESAATESLHTTPTAPQRALPQP
                     PPVAPRPAPPPAAPPASASVLDKPSDTTVAVS"
BASE COUNT         1962 a         3149 c         3284 g         2075 t
ORIGIN      
        1 ccccccacct gcgcggcgtc gtgcaatgcc gcttcacacc aacttgctcc tggtacggcc
       61 acgagtcgat ccgcaaacac ggcttcctca tcggcggcgc gaaaacgttc tggcggattg
      121 cgaggtgcgg tccgtggacg aagttgggga cgtacgatcc gccataggtt cgcaagtcaa
      181 aacgggcgtg acaatcgccg catgctcgtc tacatttgat cgtagttcca tcttgtcgct
      241 cttccccgat ttcgattcaa ctaaaggaga acggaatggc tgccaagaag aagacctcga
      301 agaagaaagc atcaaagaaa gcgaccgcga agaagaaggc tgcgaagaaa gccgcgccga
      361 agcgcgccgt gaagaaggcg ccggcgaaga agaagaaagc ggctgcaaag aaagtcgccg
      421 cgaagaaagc gcctgccagg aaagccgcga agaaagctgt cgcaaagcgg gctcccgcga
      481 agaagaagcc ggcgaagaag tctgctccga agccggcggc gccgcgtccg agcgagagct
      541 tgctaccgca gaccaccacg gcgccagctg gttctgctgc cgtggcgccc gacatggccg
      601 gcgatcaaga ggggtcagac tcggagaatg aaagaggcgc gttcgatcac ttctagcgaa
      661 gtggtttgag gaccggtggc tggcagaggg ccggcggagc tgaatctgct ccggcggctc
      721 ctccgctctc agataggctt gatgtgcagg ccggcgaacc tcgatgtacg ctcgaatctg
      781 cgccaccagc ggactcggtg aaatgttcgt cgcatagaaa ccgggcgcgc gctcgcacct
      841 cggctaccgt tcgagccgat gccctcaaaa caggacgctc tcgattcgaa agccgtcctt
      901 cgcgtcttcg cggcttcgcg tgagaagcgt taactgacat ccgatccgta ggttacaaga
      961 gcgggcatca ttgctccagg gtcgcaaaaa tacctccgag gtcgagatat ttatcctcga
     1021 ggtcgagata ttcatcctca aggtcgaggt atttatcttc gacgccgagg tatttatctt
     1081 cgaggccgag atattcatct tcgaggccga ggtatctatc ttcgaggccg aggtattcat
     1141 cttcgaggcc gaggtatcta tcttcggggg tcgaggtatc taccttcggg ggtcgaggta
     1201 tctaccttcg aggtcgagat attcaccttc gaggcccaag gtatttatct cggaaggcga
     1261 ggtatttacc tcggacgcga acctttgcat ctccgaggtc gaggtgcggt ccatccgaag
     1321 ctgggtcacg gcgcgctgga caccccggta accttccgga ctctgcgggt cacaagactg
     1381 gatcacgaaa gattgaggcc ggccggcaac acatcccctg agtagcgcgg aaccagtcga
     1441 cgtagacgat gacgatcgaa ctactctggc ggcgcctcgg cgctcgggca caactcattc
     1501 aacgcgcact gcgcgcactt tggtttgcgg gcgatgcaga tcgtgcggcc gtgggcgatg
     1561 aggcgatgcg agaagtttat ccattccttc tgggggatca gcgccatcag gtcctgctcg
     1621 atcttcacgg cgtcgtcggt ggcggtgagg ccgaggcggg cggagacgcg ggttacgtgg
     1681 gtgtcgacca cgatcccgat cgcgatcttg aaggcgtttg ataggaccac gttcgccgtc
     1741 ttgcggccga cgccggggag cgcagtcagc tcgtccatcg tgcggggaac ttcaccgttg
     1801 tgacgctcga cgatcgcttt cgaggcggcg atgacggcct tggctttgtt gcggaagaag
     1861 ccggtctgtt tgacgtcgcg ctccatctcc acctggctgg cctcggcgaa ctctttcggg
     1921 cccgggtatt tgcggaagag cgatttggtg acgatgttga cgcgggcgtc ggtggattgc
     1981 gcggagagga tggtggcgat cagcagctcg aaggcattgg tgaaatcgag cgcgcatttc
     2041 gccttcgggt acatgcgctt gaggcgaatg acgatgtcgt tggcgcgttc ctgtttcttt
     2101 gttgccatgg gaaaaaccat tcaaccacag agacactgag gacacagaga cttagaatca
     2161 gttcatgacg acgaccattg aagaagcacg cgtcattcgg gcaccgcgcg gcaccactct
     2221 ctcctgcctt ggctggcagc aggaagcggc gctgcggatg ctgatgaaca atctcgaccc
     2281 cgaggtggcg gagcgaccgg aggagttgat cgtttatggc ggcatcggca aagcggcgcg
     2341 caactgggag tgcttcgatg cgatcgtggc cacgctcaag cgtctgaaga atgatgagac
     2401 gctgctcgtg cagtcgggca agccggtggg cgtctttcgc acgcatgaaa acgcgccgcg
     2461 ggttctgatc gcgaactcca atcttgtccc gcactgggct acctgggacg agttccggcg
     2521 cctcgatgcc ctcggcctga cgatgtacgg ccagatgaca gccgggtcgt ggatctacat
     2581 cggcacgcag ggaatcctgc aaggcacgtt cgagtgcttt gcgtccgtgg cgaagacgcg
     2641 cttcgataac acactccgcg gaaagctcgt cgtcacggcg gggctgggag ggatgggggg
     2701 cgcgcaaccg ctggcggcga cgatgaacga ggcgacggcg ctggtggcag aggttgatga
     2761 gacgcgcatt caacgtcgtc tcgacaagcg ctacctcgat gagaaggcgc catcactgcg
     2821 tgcggccatc gagcgggcaa tcgcggcgcg cgatgccggc gaggcggtct cgatcggcgt
     2881 cgtcgcgaat gcggtcgagc tgctcgaagc gctgctcgag cgcaacatcg ttcccgacat
     2941 ccttaccgac cagacttcgg cgcacgacga gttgaacggc tacgttcccg cggggctttc
     3001 atacgacgcg gcgctggcaa tgcgcgacag cagcccggac gcctacatcg agttgtcgta
     3061 cgacacgatg gcgcggcaca tgcgcgcgat gctggagctg cagcgtcgcg gcgcgatcac
     3121 gttcgactac ggcaacaatc ttcgcgggca ggcgctgaaa gccggcgtga aggatgcctt
     3181 cgacgtcggc ggatttgtcc cgctcttcat tcgccctctt ttctgcgaag ggcgcggacc
     3241 gtttcgctgg gccgcgcttt ccggcgatcc gaatgatctg tttgcgactg acgaagcgct
     3301 gctcgagctc ttcccgcacg acgccgcgct gcaccgatgg atccgcatgg cgcagaagcg
     3361 cgtcgagttt cagggattgc cggcgcgcat ctgctggctc ggctacggag agcgcgacaa
     3421 agcgggcctt ctgttcaatg acttggtccg cacaggcaaa gtgaaagcgc cgatcgtgat
     3481 cggacgcgat catctcgatg caggatcagt tgcgtcgccg aatcgtgaga ccgaggcgat
     3541 gaaggatggg tcggatgcaa tcgcagactg gccgatcctc aacgcgttgc tgaataccgc
     3601 cgccggcgcg acgtgggtat cgtttcacca cggcggcggt gttggaatcg gctactcgct
     3661 gcatgccggc atggtcgttc tcgccgacgg taccgacgct gccgcagaac gtctccagcg
     3721 cgttctcacg accgatccgg gcacaggcgt gatgcgtcac gccgacgcgg gctacgagat
     3781 cgcgatcgag gcggcgaagg agcgcggcgt cgacattccg atgctgtgac gcgaagacgc
     3841 tcacgcgaag gcgcgaagac acgaaggagg aaaggatcca attcctttct tcgcggcttc
     3901 gcgtcttcgc gtgagcgggt tggtgtgatt actccaccgt tacgctcttc gccaggttcc
     3961 gcggctgatc gacgtcgcag ccgcggcgca gggcgatgta gtaggcgagc agttgcgtcg
     4021 ggacgacagt caggatcggc tggagcggtt cgatcgtcca ggggatctcg atgacgtcgt
     4081 ccgcgagcgc tttgacagca tcatcgcctt cgtcgcagat cacgatgagc ttcccatcgc
     4141 gcgccttcac ctcctgcagg ttggagacga ctttgtcgta gacgggtgaa tgcgtggcga
     4201 tggcgacgac ggggaggttc tcgtcgatca gcgcaatcgg tccatgtttc atctctcccg
     4261 ccggatagcc ctcggcgtga atgtacgaga tctccttgag cttgagcgct ccttccaacg
     4321 cgatcggata gtgaacgccg cgcccgagga agagaaagtc ctgcgacttc gaatacttgt
     4381 gcgcgagcgc ctcgatgtgc ttttcctgtt tcgccagggt gtcttcgatc ttgtgcggaa
     4441 tcacgctcag ctcgtgcatg gcgaaacggc ggtcgtcgag atccccgccg cgcagctggc
     4501 gtatgtagag cgagagaaga tagaaggcgg tgagctgcgt ggtgaacgcc ttcgtcgagg
     4561 ccacgccgat ctccggtccg gcgttcgtgt agatcacgcc gtccgacatg cgcgtcgcgg
     4621 cggcaccgat cacgttgcaa atcgtgatca atttcgcgcc gcgcttcttc gcctcctgca
     4681 tgccggcgat cgtgtcggcg gtttcgcccg actgagtcac gccaatgacg agcgtgctct
     4741 cgtcgatgat gggattgcga tagcgatact ccgatccgta gtcgacttcg atcgggatcc
     4801 gcgccgcatc ctcgagcagg aacttgccga cgagcgccgc atgccacgat gtgccgcagg
     4861 cgatgacgtg gactttcctg atctttgccc actcgtccgg cgtgaactgc aggtcgttga
     4921 agaagatctc gcccgactcc tcgaacatcc ggccggtaaa tgtgtcgcgg acggcgcgcg
     4981 gctgctcgtg gatctccttg agcatgtagt gccggtagcc ttccttctcg gccatcacgg
     5041 catcccacag aatccgtttc ggcgcgcgct cgaccttttc gtccttttcg ttgaagaccg
     5101 aaacgttctt ctgatcgacg atcgcgtatt cgccgtcctc gaggtagatg atgtcgcgcg
     5161 tgtaggcgag caacggagcg acgtcggagg cgacgatgtt ctcgttgtcg cccaatccga
     5221 cgacgagcgg cgggccatgt ttggcggcga tgatcgtgcc gggctcgttg ccgtcgatca
     5281 tgaccagcgc gtagtggccg tccaactcct tgagcatctt cgtagccgct tcgacgaact
     5341 tcgtcccatc cttcatgttc tcttcgatga gatgggcgac gacttcggtg tcggtctcgg
     5401 tcttgaactc gtgccccgct ttctgcagcc gctgcttcaa ggggaggaag ttctcgatga
     5461 tgccgttgtg aatgacgacg atggcgccgg tgcagtcgcg gtgcgggtgc gcgttgttct
     5521 cgttcggctt tccatgcgtt gcccagcgcg tgtgacccat gccgaacgta ccggagagcg
     5581 gcttctcgct cagcttcgct tcgagattgg agagctttcc ttcggcgcgc acgacgtcga
     5641 cgcggctgcc gtccacgacc gcgattccgg cgctgtcgta gccgcgatac tcgagctttt
     5701 tcagtccgcc gatcaggacc ggcacaacgt cacgagtgcc ggcgtaaccg atgattccgc
     5761 acatgaagcc tccagaacgg tgaatgacga aagaggagcg gtgaacacgc gcccggaaag
     5821 ttcatcgctc atccttcatc gttcatcgtt agaatcgcga gtaatgctgc cgaagaaact
     5881 ggtcgtcctg ctagcggtcg cggttctcgc ctgtggtaaa cgcggagatc catttccgcc
     5941 ggtgcctgtc attccgaaag cgacgaccga tcttcttgta gcacagcgtg gtcccaaagt
     6001 tcttctctcc tggagctacc cttcgctgac gacgtccgga aaaacgatga ccggcatcaa
     6061 gcgcatcgtg atctggcgat cggtcgaaga gcttccggtc gcaacgagcg ctcccccttc
     6121 accgattgaa caacccgtcc agcttttcgc gaaagttccg ccactgggac ccaatcagtt
     6181 tctgaagctc cggacaaagg tagacagcat agagagcgcc aacttgaaag cagcgaccac
     6241 cggcgcgcgg ctgacatacg aagatgcgcc accgcttcat gccgcgaacg gaaggccggt
     6301 tcggatcacg tactcggtcg tgacccaggg tccggtcgtg agtggtgatc tgtcgaatct
     6361 tcccaccatc gttccgctcg atgttccggt tgcgcctacg gctctcgccg cgactgcgaa
     6421 gaaagaagga gtcgtgctca cctggacagc gcccgagaca tcgatcagcg gcgcgaagaa
     6481 cccaccagtc gccggctaca acgtttatcg attcccgcac ggtcagcccg cggacgagtt
     6541 cgcggcgcct gcgaatccat cgcccgtcac cgcaacgacg tacaccgatc aaccgcccta
     6601 cggaacgcat gattacttcg tgaccgcggt tgcagcatca ggtactaccc gcatcgagag
     6661 cgagccatcg gcaattgccg gcgccgaatt caaggatctc gtcccgcctg cgcctccggg
     6721 cggactgaca gccctcgtcg agaccggcgc cgtccgactg gtgtgggatc cggtcgacgc
     6781 gccggatctc gctggttaca ggatctatcg tacggaagga acagggatcg agcagttgcg
     6841 ggtcgccgga acgattcctc tgaatccggt gcagttgctg acggcgacga actaccgcga
     6901 caccggagtc aatcccggca tctcctacta ctacgaggtg gtgtccgtcg ataagagcgg
     6961 caacgaaagc gcgcgatcga agacggattg ggtgctggtg ccgaagacgc cgtgagggct
     7021 tcgtagtgca ggcattcttg cctgctaccg ttgggcgtcc tcgcccgacg ggatcgtccc
     7081 aatcttcgaa atcgtccgcg tagcgacgtt tcgggtttca ctttcgattg cgaagaattt
     7141 gcgcttacca ccgccgggca gggacgcccg tcgggagcag gcaggaatgc ctgcgctacg
     7201 ttagttgacg acgtcgcagg agtcgtcgtc gagggcttgc tcgtgcggcc agcgggacgc
     7261 cagaaggcgt tcaaaatagg gccggacgat tcgttcgtag cgttgctcga atcctggatg
     7321 aaggtcgagg atcgctttgc gcgcgcagcg cacgaggcat cgcgcttcgc ggtgcgacac
     7381 ggtcatgtcg ctgccgatga ggttgagcgt gagctcgaca atcatgcgga tgcgacgaag
     7441 gcggcgccgc tcgaaaatcg gggagcggac ttctgtcgtc gaatccatcg cgtacttgct
     7501 catgctcggt taccgcgctc aaacggcgat gccttgtcta ccattccaga ctcgatgaac
     7561 attcgtatgc gacatatttt cgaaacccgg cccaccaacg attccgcctg actacgcgtg
     7621 ctcgcggtcg ccttcggccc agatctcgtc tgcgatggtc gcgtcgatct gcgattcgta
     7681 gaacttgatg gcggcgcggc cgacagcgcg aacggaaacg tagttcagcg caccgccgga
     7741 aagagcacca acgaacggga tcgcctttgt cagccggtcg cggaagaaat tcgcgccgag
     7801 cttctcggcg atcatgccgc cgtccatcgt ggccgactcc agcatcacag cgattccctg
     7861 cttgccaatt ccgacccccg aggcgtaggc gagacaggac attgcctcgg cgaaccgttc
     7921 gcgctgcgtc ggctcgtggc cgtagacggt ggcgatgccg aggatcaggc ggaactgctg
     7981 gaagacgaga aaggccagtt ccgctccgag cgtgccgatg gcgatcagtc cgctcggcag
     8041 tccggaaaca gcgccggcag cggcgcaacg gagcgctgtg cgccgcacga gcagccgcgc
     8101 atgatcgtcg gagtggaaat caggagcgct cgctttcagt gcatcgacgt cacggcggat
     8161 cttctccgta tcgatctctt cgaacagaga gcggaagacc tcctcgagca tcgagtcggc
     8221 gcgcagcttc agttcccggg ctcttcccat cgcggttcag gcggagggtt tggactccgc
     8281 cttcgattct gacttcgtct cgctcctgct ttccgatttc gtctcagact ttgactcgga
     8341 tttcgattcg gacttggaat ccgatttcga ttcagacttg gagtcgctct tcgagtcgga
     8401 tgatttgctc tcgcctgatc cttcgctctt accgtccgat ttcgattcgg acttcgaatc
     8461 cgatttcgat tccttcgagg acgcgctggc gtagtcagtc ttgtagaagc ccgatccttt
     8521 gaactggatg gcgggcgccg acatcagctt cttgacgtca ccaccgcact tgtcgcaaac
     8581 cgcgtacggc ggatccgaga acttctggat gacttccgtc ctgtcgttgc acttggtgca
     8641 ctggtactcg tagatgggca tgcgggatgt ggaacgagac gggggttagc gcgattccgt
     8701 tggagcagag atcaggggag gcagagatca gagatcaggg tatgcgtgtc gtgtcgtatg
     8761 tttctggtcg ttgatcgcag tgcccagtga cgtgagatca gaaatcggga gatcaagtca
     8821 gcagaggtgt gccgtcctga tctctgatct ctagtccctg atctctgcct ctacatcaac
     8881 ggcgccactt tagcctgcga gttttcttcc gcagcgaaga gcgcgccgtc gcggaagtag
     8941 acgccggcga tgcctccatc gatccgccgg tagtaccacg cctcgatggt cgtatcggga
     9001 cgctcggtgc ggatctgcca tcccgggatt ggcttcccca ggagatcggc gacgtcgcgc
     9061 tgcgacatcc ctttctgcag cgccagcagc ttttcgcgcg acacggccag atggttagct
     9121 gcactgctga gaccttgcat cgccgcgatg ttctgaggat cgtgagtcag aatcgcgcgg
     9181 tagatccgaa tggcgtcctg gtagcggccg agcgacgaaa gctcgcgcgc gaaatcgagc
     9241 tggatgtgct ggtagacctc gcgggcacgg ctgtgagtag ggtggttgcg gacgaaagtg
     9301 ccgagggtat cggcgtagac ctgtttggcc tgggcgggtg cattcggcgc ctgcgcggcc
     9361 tgcttgtggt ggagaacgcg caaccactcg gaggagttgt cgatggcatt gccgccgccg
     9421 caagccacgg cagcaagact gatcaggata agagcgcgtc gcatcggtac caaagtagca
     9481 cacgttctct gcgctataat ccccgggttt tgcaggggtt attcaacctt cagcattcgg
     9541 aggaagcatg gctgtgaggg gtgacgaggt cgcgggctcg atcgaggcgc acgtccgcga
     9601 cgcgatggag aagctggtgg gcgagattcg ctcctccatc gaagacgtcc gcgaggtcgt
     9661 cgactcgcag ctccgcgcgg cgctccagag cgtgcaggct gacgtcaacg ccgtctcgtt
     9721 cctgccgcac attcagaagt cgatcggcga tctggaagag agcatcacct ccgcacagcc
     9781 cgccgctgct gtgcctgcgc cctcgggcgg cgacgcctct ctcctcaaac gaagcattca
     9841 ggccgtggag cgcgggaagt cgcaagtcga catcctcaat gcccttctcg agcaatgcct
     9901 cgccttcggc tcccgcgccg cgatgctgat cctgcgcaac gagaccttca gcggctggaa
     9961 gggcgtcggc ttcaccgccg ccggcggcaa cgatgagatg gtgaaacgct tcaacgccgc
    10021 gcccggactc atccccgagc tcgaccgcgt tctgcgcagc gaagaagtcg tcctctggga
    10081 cggcgccaac ctctccaccc gcctcggcgt cccggcatcg tcgcgcgccg tcctcgtgcc
    10141 gatggtgatc aaggacaaag tggcagccgc ggtctacgtc gacgcgaccg aagacgacgc
    10201 cgcgaagctc gacgcgtcct cgatcgagct gctcgtcttc gcaaccggtc tcctgatcga
    10261 cacgctcacc atccggaaga agattccgtc gccgagcctc aacgattcgg agtccgctgc
    10321 caccgaatcg ctgcacacga ccccaaccgc cccgcaacgc gcgctgccac aaccgccgcc
    10381 ggtagctccg cggcccgctc cacccccagc cgcgcctcct gcctcggcat ccgtgctcga
    10441 taaaccgtcg gataccaccg tcgccgtttc
//
LOCUS       DATLVW010000869         7869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_119582, whole genome shotgun sequence.
ACCESSION   DATLVW010000869 DATLVW010000000
VERSION     DATLVW010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119582"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VNN25_25515"
     CDS             <1..484
                     /locus_tag="VNN25_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94958.1"
                     /translation="TFLAAASLVFYFAKYEFGPRKYPTKTLDGRCLYKPSAVAGILDQ
                     LGDSRAAYFEQETTIDLIFPMLYGLMFVSAIVGLTPGAGTPRWVVALPLITVMSDYAE
                     NTTVIAMLKRYAAARAGQAVDLGVLPWIASAVSGTKGISLLASTLAVVVLAVAWLIRR
                     "
     gene            complement(489..713)
                     /locus_tag="VNN25_25520"
     CDS             complement(489..713)
                     /locus_tag="VNN25_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94959.1"
                     /translation="MKKNDLPTTDDDMLPEYDFRGGVRGKYARDYGRNRNLRVLDPDL
                     LGNFPDSDSVNEALRTLLRVKAAIQPLPPD"
     gene            complement(740..1003)
                     /locus_tag="VNN25_25525"
     CDS             complement(740..1003)
                     /locus_tag="VNN25_25525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94960.1"
                     /translation="MSRKNAPAEDDDPLDREVDFSNAKPNPFARDYGRNRNLRILDPD
                     LLAVFPDSESVNEALRTFIRLAAATSPKDQRGDTSVSKTKKRG"
     gene            complement(1013..1294)
                     /locus_tag="VNN25_25530"
     CDS             complement(1013..1294)
                     /locus_tag="VNN25_25530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94961.1"
                     /translation="MKNKSTKKTSEATDDDPLDREIDFSKSRPNPYWLGVVDRNCVRL
                     LERDLADQFPDDKAVNEGLRALLRIKDATPQKISARTASAPKARKKARA"
     gene            complement(1294..2607)
                     /locus_tag="VNN25_25535"
     CDS             complement(1294..2607)
                     /locus_tag="VNN25_25535"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HXH94962.1"
                     /translation="MADLNVYEVLNRKRRGAPIEAREIEQFIEKYTLGEIPDYQMSAL
                     LMAIAINGMTAEEMATLTQAMLHSGDEWHLRNEYDFIADKHSTGGVGDKVSLVLSPWV
                     AACGIKIGMLSGRGLGHTGGTLDKLEAIPGFNARLSREDFNRCVAEAGCVISTSTEGI
                     APADKKIYALRDVTGTVESLPLITASIMSKKLAMGASALVLDVKTGSGAFMRKYQDSK
                     ALAQSLIAAAAGSGTRVEALITDMDRPLGIAAGNANEIIETIEVLKGNGPADVEFVTR
                     AQAQRTLVLSGKFDDASAAAALEDALRSGRALDQARAWISAQGGDAKIVDDYSLLPQP
                     KTTIEVRAPQSGFITFMDTYQAGMFTVDLGAGRKQANDVIDYAAGVMFDRRTGDEVRA
                     GETIARIQLGNASRDAAELTKRFLSFVTFGDTKPEERPLIHEHLT"
     gene            2631..3302
                     /gene="queC"
                     /locus_tag="VNN25_25540"
     CDS             2631..3302
                     /gene="queC"
                     /locus_tag="VNN25_25540"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HXH94963.1"
                     /translation="MMKAVVLLSGGLDSATVLAIARSEGRACHALSFAYGQRHEIELA
                     ASRRVAEAIGVDEHVVFPMDLRVFGASALTDDIDVPKDVAGAPGIPMTYVPARNTIFL
                     ALALGFAEARGAQEIWIGVNAVDFSGYPDCRPEFLEAFQRVIDTGTRSGVEHGEPRLI
                     APLVSMSKADIIRRGTSLGVDYAITHSCYDPDAAGRACGHCDSCILRKRGFEEAGVAD
                     PTQYR"
     gene            3476..4111
                     /gene="queE"
                     /locus_tag="VNN25_25545"
     CDS             3476..4111
                     /gene="queE"
                     /locus_tag="VNN25_25545"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HXH94964.1"
                     /translation="MLRITEIFHSIQGESSHVGRPCVFVRLAGCNLRCRWCDSEYTFT
                     GGEKSSIDDVVERVRSYGCNLVEVTGGEPLAQKEAFALIEKLCDDGFEVLIETSGSID
                     IEPVDRRAKIILDIKCPGSGEAEKNYWPNIENLRPHDEIKFVIADRADYEWARGLIAE
                     RNLVGRTILFSPVWGELELKPLAEWMLADRVPARLQTQLHKHIWGADVKGV"
     gene            4111..4509
                     /gene="mce"
                     /locus_tag="VNN25_25550"
     CDS             4111..4509
                     /gene="mce"
                     /locus_tag="VNN25_25550"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010478988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXH94965.1"
                     /translation="MTKPTLDHIGIAVKSLDAAKIYEALGLTIEHIETVESQGVRTAF
                     LAAGDAMIELLEPSGPQSTIAKFIEKRGEGIHHICFRVDDIEAQLARLQQQGYRLINE
                     SPVPGAHGCRVAFLHPAAGNGVLIELSEKQ"
     gene            4506..4838
                     /locus_tag="VNN25_25555"
     CDS             4506..4838
                     /locus_tag="VNN25_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94966.1"
                     /translation="MIEGFAPASLVIVNLVNPKEKFFGVLRALSPAGVTIKGINLDSY
                     EDWIHQIARNDDLDLNLVTMFVPLFRVERIFLDEPSGMIKSHSQRFEEVVGISIGTFL
                     GLDEQYDH"
     gene            4903..5562
                     /locus_tag="VNN25_25560"
     CDS             4903..5562
                     /locus_tag="VNN25_25560"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HXH94967.1"
                     /translation="MKPFRIALVLMCFTAFPALAADHVFDLTGWAAWIDPNSNGTFNA
                     SFPNQPFDVRFDGKMGYGIGANIFFGNHFSAAFDVAQVRPGTTLRTRAVGGTSFTTTG
                     TRMTPITGILQWHFAPGGGIDPYIGAGAAYVVFDKTNVFKSATLSQINIKDDAGLALN
                     AGVSFRLSRMLALTADGKYVPLKAGATPVYAPGTTPIVPITLNAKINPVIFSGGLTLR
                     F"
     gene            complement(5567..6511)
                     /locus_tag="VNN25_25565"
     CDS             complement(5567..6511)
                     /locus_tag="VNN25_25565"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXH94968.1"
                     /translation="MAYYLGVDGGASKTAALVTDETGRPLGSGVAGPSNHLRVGIETA
                     ARNIERAVNKALVTADVASREIVWTYCGIAGADHPAHRQEVVDSLSVFFPRGNFTVDN
                     DARIALTGAIGFGAGIVIIAGTGSVAIGRNDAGEEARVGGWGPIVGDEGSGYAMGRGG
                     LAAILRAFDGRGPATAMTALLQRDYQMTPADLPRYVYAQTTHADDIARLSKLVIDAAE
                     NGDAIARGLVDSAGSELAKSVIAVAQRLGLIASDFPVAYVGGAFKAGETLLAPMRNTI
                     LEAAPHAHLSPPVRTPVEGAAQMAMRAATNPRPTRAVE"
     gene            6566..6844
                     /locus_tag="VNN25_25570"
     CDS             6566..6844
                     /locus_tag="VNN25_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94969.1"
                     /translation="MVDDRRGNNAAVKESSKSGWRELAPGPIALARISGLIAGISLLL
                     AKISGEKRDFQTKNAIFIRKTRFSREKHDFRRKNTLARRMNAIFVRKT"
     gene            complement(6848..7162)
                     /locus_tag="VNN25_25575"
     CDS             complement(6848..7162)
                     /locus_tag="VNN25_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94970.1"
                     /translation="MLHTGRVVMLTSPHPFFQQPNPMASMRVLGQGQLTTDNVIVQRL
                     VATVIRRLSRQTVVLSRFFAENHVFRLKITFFAWKSRFFAENRVHPPCQRVFRVEIAF
                     FA"
     gene            7282..7764
                     /locus_tag="VNN25_25580"
     CDS             7282..7764
                     /locus_tag="VNN25_25580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94971.1"
                     /translation="MKSHRISTAAAVALFVVALIAVAGCKTSGMAVTRHGQLLKINIE
                     HPMDLPDRGEDNLNVVVGNRGVRSVKDVLIDVEVPPELVVLDETHERGVTMSHEPGSN
                     FYHYTMGNLQPAEDSTIHYKVRTSFGTRSESGNVSVTAWQRDLPGDKLVETALIKLRP
                     "
BASE COUNT         1459 a         2348 c         2489 g         1573 t
ORIGIN      
        1 taccttcctg gcggcggcgt ccctcgtctt ctatttcgcg aagtacgagt tcgggcctcg
       61 gaagtatccg acgaagacac tcgacggccg ctgcctctac aagccgtccg cggttgccgg
      121 catcctggac cagctcggcg actcacgcgc cgcgtacttc gaacaggaaa cgaccatcga
      181 tctgatcttc ccgatgctgt acggcctgat gttcgtctcg gcgatcgtag gactcactcc
      241 cggagcgggt acgccgcgat gggtcgtcgc gttaccgctg atcactgtga tgagcgacta
      301 cgcagagaac acgaccgtga tcgcgatgct gaaacgatac gcagccgctc gcgcagggca
      361 ggcagtggat ctcggggtgc ttccgtggat tgccagcgcc gtttcgggaa ccaaaggcat
      421 ctcattgctc gcctcgacgt tggccgtagt cgtgctcgcg gtcgcctggc ttatcaggcg
      481 gtgagtcatc aatccggtgg cagcggttga atcgccgctt tcacgcgaag cagcgtccgt
      541 agcgcctcgt taaccgaatc ggagtcggga aagttgccca gaaggtcggg atcgaggacg
      601 cgaagattgc ggttgcggcc gtagtcgcgc gcgtactttc cgcgtactcc gccgcggaag
      661 tcgtactcgg gcagcatgtc gtcgtcagtc gtgggcaggt cattcttctt cataggattg
      721 cattcagcgt tccggccatt catccgcgtt ttttcgtttt cgataccgag gtatctccgc
      781 gctgatcttt cggggaagta gccgccgcca aacggatgaa agtgcgtagt gcctcgttca
      841 ccgattcgga gtcgggaaag acggccagaa gatcgggatc gaggatgcga agattgcggt
      901 tgcggccata gtcacgcgcg aacggattgg gcttggcgtt gctgaagtcg acttcacgat
      961 ccagcggatc gtcgtcttca gctggcgcgt ttttcctgct catcgaatct cgtcaggcac
     1021 gggccttctt tcttgccttt ggcgcggagg cggtccttgc gctgattttc tgcggtgtcg
     1081 cgtctttaat ccgaagcagc gctcgaaggc cttcgttcac ggctttatcg tcgggaaact
     1141 ggtccgcgag atcacgctcc aggagacgca cacagttgcg gtcgacaacg ccaagccagt
     1201 aaggattcgg ccggctcttg ctgaagtcga tctcgcgatc gagaggatcg tcgtcggttg
     1261 cttctgaagt tttcttcgtt gacttgttct tcatcacgtc aaatgctcgt gaatcagcgg
     1321 ccgctcctcc ggcttcgtat ctccgaacgt tacgaatgac aggaaacgct tcgtcagttc
     1381 ggcggcatca cggctggcgt tgccgagctg gattcgggcg atcgtctcgc cggcgcggac
     1441 ctcatctccg gttctgcggt cgaacatcac gccggcggcg tagtcgataa cgtcatttgc
     1501 ctgcttgcgg ccggcgccga ggtcgacggt gaacatgccc gcctggtagg tgtccatgaa
     1561 ggtaatgaag cccgactgcg gcgctctcac ttcgatggtc gtcttcggct gcgggaggag
     1621 cgagtaatcg tcgacgatct tcgcgtcgcc accctgcgcg ctgatccagg cgcgggcctg
     1681 atcgagggca cggccggagc gcagcgcgtc ttcgagcgct gcggcagctg aggcgtcgtc
     1741 gaacttgccg gagaggacga gcgtgcgctg cgcctgggcg cgggtcacga actcgacgtc
     1801 tgccggacca ttgcccttca gcacttcgat cgtttcgatg atctcgttgg cattgccggc
     1861 ggcgattccg agcggccggt ccatgtcggt gatgagcgcc tcgacgcgtg tgcccgatcc
     1921 cgcagccgcc gcaatgaggc tctgcgccag cgccttcgaa tcctgatatt tgcgcatgaa
     1981 cgcgccgctg ccggtcttga cgtcgaggac cagggccgat gcgcccatgg ccagcttctt
     2041 cgacatgatc gacgcggtga tgagaggcag cgactcgacg gtgccggtga cgtcgcgcaa
     2101 cgcgtagatc ttcttgtcgg ccggagcgat cccttccgtc gacgtcgaga tcacacagcc
     2161 cgcctcggcc acgcaacgat tgaagtcctc gcgcgacagc cgtgcattga agccggggat
     2221 agcctccaac ttgtcgagtg tgccgccagt gtggccgagg ccgcggccgg agagcatgcc
     2281 gattttgatg ccgcacgcgg caacccacgg cgaaaggacg agcgacactt tgtcgcccac
     2341 gccgcccgtg gagtgcttgt cggcgatgaa gtcgtactcg ttgcgcagat gccactcgtc
     2401 tccgctgtgc agcatcgcct gcgtcagcgt ggccatctcc tcggcggtca tgccgttgat
     2461 ggcgatggcc atcagcagcg cggacatctg gtagtcggga atctcgccga gcgtgtactt
     2521 ctcgatgaac tgctcgattt cgcgcgcttc gatcggtgcg ccgcggcgct tgcggttgag
     2581 gacttcgtaa acgttcaggt cagccatagg agcgcgattg tagtattcag atgatgaaag
     2641 ctgtcgttct gctgagcgga ggactcgact ccgccaccgt actggccatc gctcgcagcg
     2701 aagggcgagc ctgtcacgcg ctctcgtttg cgtacggaca gcgccacgag atcgagctcg
     2761 ctgcgtcgcg ccgcgtggcg gaagcgatcg gcgtcgacga gcacgtggtc tttccgatgg
     2821 atctccgcgt gttcggcgcg agcgcgctta ccgatgacat cgacgtgccg aaggatgtcg
     2881 ccggcgcgcc gggcattccg atgacgtacg tccccgcccg caacacgatc ttcctcgccc
     2941 tggccctcgg cttcgcagaa gcgcgcggcg cccaggagat ctggatcggt gtcaacgcgg
     3001 tcgatttcag cggctacccc gactgccgtc ccgagttcct cgaggccttt cagcgtgtga
     3061 tcgatactgg tacgcgatcc ggcgtcgagc acggcgagcc gcgattgatc gctccgctgg
     3121 tgtcgatgtc gaaggcggac atcatccgcc gcggaacctc cctcggcgtc gactacgcca
     3181 tcacgcactc ctgctacgac ccggatgctg ccggtcgagc ttgtggtcat tgcgattcgt
     3241 gcattttgcg gaagagaggg ttcgaagaag cgggagtcgc tgatccaacg cagtatcggt
     3301 agcggcgcgt ggacgtaggt cggactctct agtccgactc ggtccgatca gtctgatccg
     3361 tccgaatgcg aagaagtgtc ggactggaga gtccgaccta cgttgtccga actacgttcg
     3421 ttcagggtga caggtggggt gacaggttgg ggcggatcgc gcatgattca acgccatgct
     3481 tcgcatcacc gagattttcc actccattca gggcgaatcg agccacgtcg gccgtccctg
     3541 cgtttttgtc cgcctcgccg gatgcaacct tcgttgtcgc tggtgtgact cggagtacac
     3601 gttcacaggc ggggagaaga gctcgatcga tgacgtggtc gagcgcgttc gcagctacgg
     3661 gtgcaatctt gtcgaagtca ctggcggcga gccgctggcg cagaaggaag cgttcgcgct
     3721 catcgaaaaa ctctgcgacg acggtttcga agttctgatc gaaacctcgg gatcgatcga
     3781 catcgagccg gtcgaccggc gcgcgaagat cattctcgat atcaagtgtc ccggatcggg
     3841 ggaggccgaa aagaactact ggccgaacat cgagaacctg cggccgcacg acgagatcaa
     3901 gttcgtcatc gctgaccgtg cggactacga gtgggcccgc ggtttgatcg cagaacgcaa
     3961 tctcgtcggg cgaaccattc ttttttcgcc cgtctggggc gagctggagt tgaaaccgct
     4021 ggcagaatgg atgctcgcgg accgcgttcc ggcgcggctg cagacgcaac tgcacaaaca
     4081 catctggggc gcggacgtga agggagtttg atgacgaagc caacactcga ccacatcgga
     4141 atcgccgtga agtcgctcga cgcggcaaag atctacgagg cgctcgggct cacgatcgag
     4201 cacatcgaga ccgtcgagtc gcagggcgtg cgcactgcgt tcctggccgc gggcgatgcg
     4261 atgatcgagt tgctcgagcc gtcggggccg cagtcgacga tcgcgaagtt catcgagaag
     4321 aggggcgaag ggattcatca catctgcttc cgcgtcgacg acatcgaggc gcaactggca
     4381 cggctgcagc agcagggcta ccgtttgatc aacgaatcgc ccgttccggg tgcgcacggc
     4441 tgccgcgtgg cgtttctcca tcccgctgcc ggcaacggcg tgttgatcga gctgagcgaa
     4501 aaacaatgat cgaaggattc gcaccggcct cgctcgtgat cgtcaacctc gtcaatccga
     4561 aggagaagtt cttcggcgtg ctgcgggcgc tttcccctgc cggcgtgacg atcaaaggca
     4621 tcaatctcga ctcgtacgag gactggattc atcagatcgc gcgaaacgat gatctcgatc
     4681 tcaacctggt cacgatgttc gtgccgctct ttcgcgtcga gcggattttc ctcgacgagc
     4741 cgtccggaat gatcaagtcc cacagccagc ggtttgaaga ggtcgtcggg atttcgatcg
     4801 gtacgttcct cggtctggac gagcagtacg atcattagtt ttccgatcgg atactgctgc
     4861 ggtcacagtt tttgctttcg ctccgcgctg gaggattgca cgatgaaacc gtttcgcatt
     4921 gcgctcgttc tcatgtgttt caccgcgttc cctgcgctcg cagcggatca tgtgtttgat
     4981 ctgaccggat gggcggcctg gatcgatccc aacagcaacg gcacgttcaa cgcgtcgttt
     5041 cccaaccagc cgttcgacgt caggttcgac ggcaagatgg gctacggaat cggggcgaac
     5101 attttcttcg gcaaccactt ctcggccgcg ttcgatgtcg cgcaggtgcg gccgggaacg
     5161 acgcttcgca cgcgcgctgt cggtggcaca tcattcacca ccaccgggac gcgcatgact
     5221 ccgatcacgg gcatcctgca gtggcatttc gcgcccggcg gcggcatcga tccgtacatc
     5281 ggcgcgggcg cggcctacgt cgtgttcgac aagaccaacg tcttcaaaag cgccacgctc
     5341 agtcagatca atatcaagga cgacgccggt ctggcgttga acgccggagt ctccttccgc
     5401 ctctcgcgca tgctggcgct cacggcggac gggaagtatg taccgctcaa agcgggcgcc
     5461 acacccgtgt acgcgccggg tacgacgccc atcgttccga tcactctgaa cgcaaaaatc
     5521 aaccccgtga tcttctcggg cggcttgacc ttgcgcttct gagcgattac tcgacagcgc
     5581 gcgtcggccg tggattcgta gccgcacgca tggccatctg ggcagcacct tcgacgggcg
     5641 tgcggacggg tggggagagg tgggcgtgcg gggcggcctc gaggatggtg ttgcgcatcg
     5701 gcgcgaggag cgtctcgccc gccttgaatg cgccgccgac gtacgcgacg ggaaagtcgg
     5761 aggcgatgag cccgagccgc tgcgccacgg cgatgaccga tttggccagc tcgcttccag
     5821 cactatcgac gaggccgcgg gcgatcgcat ctccattctc cgcggcgtcg atcaccagtt
     5881 tgctgagccg ggcgatgtcg tcggcgtgcg ttgtttgcgc gtaaacgtag cgtggcagat
     5941 cggcaggggt catctgatag tcgcgttgca gcaatgcggt catcgccgtc gccggtcctc
     6001 gcccgtcaaa cgccctcaga atcgccgcca acccacctcg gcccatcgca tagccgctcc
     6061 cttcgtcacc gacgatcggg ccccatccac ccacccgcgc ctcctcgccg gcgtcgttgc
     6121 ggccgatggc aacggagccg gtgccggcga tgatgacgat gccggcgccg aagccgatcg
     6181 ctccggtgag cgcgatacgg gcgtcgttgt cgaccgtgaa gttgccgcgc ggaaagaaga
     6241 cgctgagcga gtcgaccact tcctggcgat gcgcaggatg atccgcgccg gcgatgccgc
     6301 agtacgtcca gacgatctcg cggctggcca catcggcagt cacgagcgcc ttgttgaccg
     6361 cgcgctcgat gttgcgtgcg gccgtctcga tcccgacgcg gagatgattg gaaggtccgg
     6421 ccacgccgga gccaagcgga cggccggttt cgtccgtgac cagcgctgcc gttttcgaag
     6481 cgccaccatc gacgccgagg tagtacgcca tgcggaagag tcttttaccg caagtgtggt
     6541 gcctttgtgc ggttacgcgg ctgcgatggt tgacgatcgt cgcggaaaca atgccgccgt
     6601 gaaggaatcg tcaaaatcag gttggcgaga gttggctcca ggtccgatag cgttggcgag
     6661 aatctcgggg ttgatcgcgg gaatctcgct gttgttggcg aaaatctcag gcgaaaaacg
     6721 cgattttcag acgaaaaacg cgattttcat ccgaaaaaca cgattttcac gcgaaaaaca
     6781 cgattttcgt cggaaaaaca cgttagcacg gcggatgaac gcgattttcg tccgaaaaac
     6841 gtgattgtca ggcgaaaaac gcgatttcca cgcgaaaaac gcgttggcag ggcggatgaa
     6901 cgcgattttc agcgaaaaaa cgcgacttcc aagcgaagaa cgtgattttc agacgaaaaa
     6961 cgtgattttc agcaaaaaaa cgcgacagca cgacggtttg ccgcgacaat cggcggataa
     7021 ctgtcgcgac aagccgctgg acgatcacgt tgtccgtcgt cagctggcct tgcccaagca
     7081 ccctcataga cgccatcggg ttcggctgct ggaagaacgg atgcggtgag gtgagcatca
     7141 cgacgcggcc tgtgtgcagc acgatgcgtg cgagtgcggc cggaaatgca ccggggatgt
     7201 ccagacaaga aagacagcat cggcgccgcg aaaatagctc aggaccgctc cttgcagagt
     7261 ctgctgctga aggagacgtt catgaaatcg catcgaatct ctacggccgc ggcagtagcg
     7321 ctgttcgtcg tcgccctcat cgccgtcgcg ggctgcaaga caagcggcat ggcggtaacg
     7381 aggcacggcc agcttctgaa aatcaacatc gagcatccga tggatctacc cgatcgcggc
     7441 gaggacaatc tcaacgttgt cgtcggcaac cgcggagtgc ggagcgtcaa agacgtcctg
     7501 atcgacgtgg aggtgccgcc ggagctcgtc gttctcgacg agacgcatga gcgtggcgtg
     7561 acgatgtccc acgagccggg ctcgaatttc tatcactaca cgatgggcaa cctgcaacct
     7621 gccgaggact cgacgatcca ttacaaggtg cggacgtcgt tcggaacgag gtcggaaagc
     7681 ggcaacgtca gcgtgacggc gtggcagcgc gatcttcctg gcgacaagct ggtggagacc
     7741 gcgttgatca agctgaggcc gtagtgggtg ggggaatggg gggttgggag tggggagtgg
     7801 gagaatagct ctcagcagtt tttttctctc gatcctcgat ctaccactcc ccactcccca
     7861 ctccccact
//
LOCUS       DATLVW010000870         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_162153, whole genome shotgun sequence.
ACCESSION   DATLVW010000870 DATLVW010000000
VERSION     DATLVW010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162153"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1829)
                     /gene="asnB"
                     /locus_tag="VNN25_25585"
     CDS             complement(12..1829)
                     /gene="asnB"
                     /locus_tag="VNN25_25585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXH94972.1"
                     /translation="MCGINAIFAYRDAAPAVDREEVIATRECMHSRGPDAGDAWFSDD
                     GRVGFGHRRLAIIDLSPGGAQPMHFGALSIVFNGEIYNYRELRAQLEARGRRFTTQSD
                     TEVLLQLYDEKQERMLDELRGMFAIALWDGARRRMLLARDPYGIKPLYYADDGGTIRI
                     ASQVRALAASGKVSKQFDPAGAAGFFLRGTVPEPFTMYRAIRALPAGSYAYVDANGVR
                     EPVQYFSIAATLRDAVENGGTFSEEQRRGIVHDAVLESVRYHMVADVPVGAFLSAGID
                     STAVVALARESGASDLQTMTLRFEEYRGRVNDEAPLAAMVAQQYGVRHAIQELTLPQF
                     RAELPRIFAAMDQPSVDGLNSFFISKAAHDLGLKVAMSGTGGDELFGGYTSFRDIPRW
                     MPLTSVLSRVPKLGDGVQRLNAALAKRSRHISPKMGEIVRYGGSYAGAYLVKRGRFLA
                     SELPSLLGDEIAREGLARLDLLRLIEQTVTPDPGNGFARVAALESSLYLRNQLLRDMD
                     WASMAHSLEVRVPLVDAHLLRKVAPALVTRRERGKQLLASAARPPLPAEVQQRRKTGF
                     TLPIKEWLTQEASGKVEFGKRSWARKVYEVMFANDRGYW"
     gene            complement(2379..>2657)
                     /locus_tag="VNN25_25590"
     CDS             complement(2379..>2657)
                     /locus_tag="VNN25_25590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94973.1"
                     /translation="RQYLPRLRGNFTQRIDGGIREVRETYSDGSRGDFQVAPDGKFTF
                     AVPLPDGPGIYTVVVWVRPHHSSGDAIPASNVSIKVERGAGTLVSGTR"
BASE COUNT          493 a          806 c          888 g          470 t
ORIGIN      
        1 ctgatcgacc gctaccagta ccctcggtcg ttcgcgaaca tgacctcgta caccttgcgc
       61 gcccagcttc gtttgccgaa ttcgactttg ccgctggctt cctgcgtgag ccactctttg
      121 ataggtagtg tgaagcctgt cttcctgcgc tgctgcactt ccgcggggag gggcgggcgg
      181 gcggcggagg cgaggagttg cttgccgcgc tcgcggcggg taacgagtgc gggggcgact
      241 ttgcgcagaa gatgggcatc gacgaggggg acgcgtactt cgagggagtg agccatcgag
      301 gcccaatcca tgtcgcgcag gagctggttg cgcaggtaga gcgatgattc gagtgcggcc
      361 acgcgtgcga agccgttgcc gggatcgggc gtgacggttt gctcgatgag gcgaaggaga
      421 tcgaggcggg ccaggccttc gcgcgcgatc tcgtcgccga ggagcgaagg caattcggaa
      481 gcgaggaagc ggccacgttt gacgaggtag gcgccggcgt agcttccgcc gtacctcacg
      541 atctcgccca tcttcgggct gatgtggcgg ctgcgtttcg ccagcgctgc gttgaggcgc
      601 tgcacgccgt cgccgagctt cggaacgcgc gacaggaccg aggtcagtgg catccagcgg
      661 gggatgtcgc gaaaggacgt gtagccgccg aacagctcat cgccgccggt gcccgacatc
      721 gcgactttca gtccgaggtc gtgggcggct ttgctgatga agaagctgtt caggccatcg
      781 acgctcggtt gatccatcgc cgcgaaaatg cgtggcagct cggcgcgaaa ttgcggcagc
      841 gtcagctcct gaatggcatg gcgtacgccg tattgctgcg cgaccatcgc agccagcggt
      901 gcttcgtcgt tcacgcggcc gcggtactcc tcgaagcgga gcgtcatcgt ctgcaggtcg
      961 ctggcgccgc tctcgcgcgc gagggcgacg acggcggtgg agtcgatgcc ggcggagaga
     1021 aaggcgccaa ccggcacgtc ggcgaccatg tggtaacgaa cggattcgag cacggcgtcg
     1081 tgaacgatgc cgcggcgctg ctcttcgctg aaggtgccgc cgttctcgac ggcgtcgcgc
     1141 aacgttgccg cgatcgagaa atactgcacc ggctcacgaa cgccgtttgc gtcgacgtac
     1201 gcatacgatc cggcaggcag cgcgcggatg gcgcggtaca tcgtgaacgg ctcgggcacg
     1261 gtgccgcgca gaaagaagcc ggccgctccc gcgggatcga attgcttcga caccttgcca
     1321 cttgccgcga gcgcgcgcac ctgcgaagca atgcggatcg tgccgccgtc gtccgcgtag
     1381 tacagcggct tgatgccgta cgggtcgcgc gccagcagca tgcgccggcg cgcgccgtcc
     1441 cagagcgcga tggcgaacat gccgcgcaac tcgtccagca tccgctcctg cttctcgtcg
     1501 tagagctgca gcagcacctc ggtgtcggac tgggtcgtga agcggcgtcc gcgggcctcg
     1561 agctgtgctc gcagctcacg gtagttgtag atctcgccgt taaagacgat cgacagcgcg
     1621 ccgaagtgca tcggctgtgc gccgcccggg gagagatcga tgatggccag gcggcggtgg
     1681 ccgaatccga cgcggccatc gtccgaaaac caggcatcgc cagcgtcagg cccgcggctg
     1741 tgcatgcact cgcgcgtcgc gatcacttcc tcgcgatcga cggccggagc cgcgtcgcga
     1801 taggcgaaga tggcgttgat gccgcacact ggcgggcgag tatacgtagg tcggggctcg
     1861 ggctagcttc cgcagacttg ggcccagccg ccaagcttga ccggcccggt agcgccgcat
     1921 cgtgccaccg ggctcacgct tagctttctc ggagccgcac tcgcggtcac gacgatgggg
     1981 aatggtgacg aaggtcgcgg cacccgtgcc accgtcaagg aatcatcaac attcgctagt
     2041 cgagggttgt tcgaagttca aaatcgttga aaacaatgtc tttaacgatg aagacaatct
     2101 ctctgacgtt gcagacaatc tctacaacat tacagtcaaa cgactacaac gttagagtca
     2161 aacgactgca acgatgcgtc aaacgactac aacgttacag acgatgtctt caacgttaca
     2221 gaccaatctc caggacgtta cagacaatct ctctaacgct acagacgatc tctgtgatgt
     2281 tgatgacaaa acgtatcggg agttgggagt cgggagtcgg aggaagaaat ggggagatcg
     2341 ctccgacacc cgactcccga tcaggactcc cgaaccactc agcgcgtgcc gctgaccaac
     2401 gtccccgcgc cgcgctcgac cttgatcgag acgttgctgg ccgggattgc atcacccgag
     2461 gaatggtgcg ggcggaccca gacgacgacg gtgtagattc cgggaccgtc aggcagcgga
     2521 acggcgaagg tgaacttgcc gtcgggtgcg acctgaaaat cgccgcggct gccgtcgctg
     2581 taggtctcac gcacttcccg gatgccgccg tcgatgcgct gcgtgaagtt tcctcttaga
     2641 cgcggcagat actggcg
//
LOCUS       DATLVW010000871        10124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_406591, whole genome shotgun sequence.
ACCESSION   DATLVW010000871 DATLVW010000000
VERSION     DATLVW010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406591"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VNN25_25595"
     CDS             complement(<1..1127)
                     /locus_tag="VNN25_25595"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXH94974.1"
                     /translation="MRMMRVAVSVFVLLLATSAGFAQSKERADIPESSKWKITDLYAS
                     DDAWRVAKDKLAARIPEVKKFKGTLGTSPQQLAAGLEMVSDLNKDFTRIAIYAGLSSD
                     QDTRVAKYRAMKQEMDQLESSFDAEKSFVEPEILKMDRATIDAFIAREPKLVPYTFYL
                     HDLLRRQAHTLSEPEEKIIADAGLMSSAPAEVYGVFTNADFPFPTVTLSDGTTRKLDL
                     ATFAVSRTLPNREDRKKVFDAFFGALGDYRGTIGTALNAEVQKDLFYMKVRKYPTTLD
                     AALDADTIPTSVYTKLIEGVNANLPTFHRYLKLRKRILGINALHYYDLYAPLVANAKL
                     EYSLDASKKNILGALAPLGTGYTSVIERAFNDRWIDLYPNAG"
     gene            1386..1649
                     /locus_tag="VNN25_25600"
     CDS             1386..1649
                     /locus_tag="VNN25_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94975.1"
                     /translation="MFIRHAVAFIGRKTRFSGENRVHPACGRVYQPKNTFFRRKSRSS
                     GVRSRLSAEKRVYPPKIAFIRRKSRFSGENRVFPLKNAFIRVT"
     gene            complement(1718..2752)
                     /locus_tag="VNN25_25605"
     CDS             complement(1718..2752)
                     /locus_tag="VNN25_25605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH94976.1"
                     /translation="MAERDLIERLDDAIEAILAGRREGLALAEPELATLLMIAGDLHG
                     LPDPDFRSKLRAELMPPMEDDMSTMTAEEVTTEFPSIRPYLLAPDADGLIAFLQETLN
                     AELLGRYPDAAGRVMHAALRVGDSLIEMGEPAGEYKARPMALHIYLDDVDGAYNRALA
                     AGATSTHPLTDQAYGDREGGVKDRWGNVWYFATHQENVTEEELQLRFAGAGGKPRKEA
                     GVGPRPAGYHTVTPFVHARGARNLITFLENAFGAKVEHVTEMPGREVAHAGVRIGDSM
                     IELGEAHGESQPIPAVIHLYVPDADATYERAVRAGARSVEAPADKPYGERAAFVIDPF
                     DNQWFIATPK"
     gene            complement(2745..3284)
                     /locus_tag="VNN25_25610"
     CDS             complement(2745..3284)
                     /locus_tag="VNN25_25610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH94977.1"
                     /translation="MDRETYDRERLLIEAAKADPSRFAELYETHFNRVWAFVIRRVRE
                     RAAAQDVTSEVFHKALKNLKSYEIRDVPFVAWLYRIAANAVNDHFNSTAREQSPDERL
                     VESEAPHDIEHIERRASLYRCVDRLPADQRRVLVMRFGEERSIREVASAIGKSEGAVK
                     QLQWRGLQSLRAQMGENHG"
     gene            3346..3810
                     /locus_tag="VNN25_25615"
     CDS             3346..3810
                     /locus_tag="VNN25_25615"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HXH94978.1"
                     /translation="MQRRLLIVLLLLIPAGSAFSQAAPEKTMPSWMAGAWASDYDGVR
                     MEEHWTQGDGGLMVGMHQDLTADGKSSFEFLRIERRDGKLVYLAMPHARPETPFPAKS
                     VEATRIVFENPDHDFPQRIIYWRDGARLCARTEGAIKSKTVSEEWCWRRMTP"
     gene            3807..5006
                     /locus_tag="VNN25_25620"
     CDS             3807..5006
                     /locus_tag="VNN25_25620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94979.1"
                     /translation="MSGERTAPSDPRHPTPDTLRRIDTPFAPAVLIAIAYTVFFLLLL
                     HARHDDVSLFVVAGGVNVDARKVPPDLTVIPNIGGYDGITFYRFALDPFTAARTEFGI
                     TLDTPPYRNQRIAYPFIVWLLSLGHARWVPALLVIVNIAAAAIMAAFGGAFSRRLGYH
                     ALWGVILPLYPGFLLTLSRDLAEIVASMFAMGAIWAISARRNLTAAILLTCAALTREP
                     TLLLAFALAGAWVIERLLRRDRRIAPVVFLAPIVIDVLWQVFLTARWGTSPLRSGAPT
                     MALPFVEFARFFAASVPRRIQQQRLYFLECLLLVSTVLTVVLMWRRTRTPLEWRFAWL
                     GYLALASVLPHTIWLEDYGFLRIFSDLFVVSAAIIIASTNGARWFALITAGGVWLYLA
                     RILVKLG"
     gene            complement(5096..5467)
                     /locus_tag="VNN25_25625"
     CDS             complement(5096..5467)
                     /locus_tag="VNN25_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXH94980.1"
                     /translation="MNTEDITVILKRFENPDETRFLVKGKFEVVHLGAMTIGRATYEP
                     GWRWSDHVGPAVGTTRCNVGHVGMVLAGIATVAFDDGRVIELHAGDLFHVPTVPHDSW
                     VVGEEQYISLHFMGADQYATK"
     gene            complement(5487..5846)
                     /locus_tag="VNN25_25630"
     CDS             complement(5487..5846)
                     /locus_tag="VNN25_25630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94981.1"
                     /translation="MRQFAGEDAIVLPEVFECPQCQRQFVFELVENTDDRSIYCPQCK
                     RYLGEKDLNFVLVSEIEELIERLRFERERLEKDEAVYAMSDCDAELKVYGTEVHKVVH
                     VLRKAVAELDALKEQYK"
     gene            5957..6030
                     /locus_tag="VNN25_25635"
     tRNA            5957..6030
                     /locus_tag="VNN25_25635"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5989..5991,aa:Gln,seq:ctg)
     gene            complement(6460..6594)
                     /locus_tag="VNN25_25640"
     CDS             complement(6460..6594)
                     /locus_tag="VNN25_25640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94982.1"
                     /translation="MQDVLAGRTRPKTRTRRSPTRVSSTCAACNGLLVGDIKKGKYVY
                     "
     gene            complement(6784..8313)
                     /locus_tag="VNN25_25645"
     CDS             complement(6784..8313)
                     /locus_tag="VNN25_25645"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HXH94983.1"
                     /translation="MTQIPTPLPQLKATGNYLTDPDGRVVILRGFNLCSKTAQSPEQL
                     GFLENNAAFLQRCGVSVVRLGLPWTNVQYVDETTRSLQYNMTFLESIKGTIELLAGFG
                     IYTLVDFHQDGYAAPWGFGAPSWAIVTEGTTMPIYGWMVNTFGGDTFVLNGERTNIQT
                     DLNSAFDFFWADAPILDGTTTLWKAYGHMLNFVSGYLKDQQGNILGYDPINEPEPGTY
                     WTEGYVPPCNDPNHVPPCNDQNPLNFSNGYPKFDPEYLGAFYQQCAIPALQNGHPGAM
                     IWFEPNIYFDYNAPTSLPDLTSVGNLGFNFHNYENTSATPFVAPISNAQKYQASWEVP
                     PPLLCSEFGGSTNYPEIQFVADLNDQNMLSAIFWAWFNNAQYHFFNNVDAAAMGVVQD
                     MSQPLEPPNLNQTMLDILTRVYPRLISGTPISFGTTRPSQTFGPITRAAYWNVNATFT
                     FTFAPGPGTTVIVVPPDLYPGDYDVVGSIGVNSTKFDGYVEITVDSPLPESVFVTIQP
                     M"
     gene            8395..8661
                     /locus_tag="VNN25_25650"
     CDS             8395..8661
                     /locus_tag="VNN25_25650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94984.1"
                     /translation="MKEPIIAPEVEVKEADLILAPEVDAISNDREVLATPRPTIDRVV
                     PEKHLRETLRLLHVDLENKSEAHGASTAVRTSSSTPSQRRRAAA"
     gene            8846..>10124
                     /locus_tag="VNN25_25655"
     CDS             8846..>10124
                     /locus_tag="VNN25_25655"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXH94985.1"
                     /translation="MAMRFRLASLVVLFFSLVLHAQQGTTVRGRVTIASDGAPLPGVT
                     VSINELNLSTVTDAEGRYQIPVPSAHTGQTVKVSAALQGFQTHTATATLGAAEVTLDF
                     PLRVSFGQEITVGSRAVNAEAEKAVPVDIITHAQIESAPSTETNQIIQKVAPSFNFPR
                     PTLSDGTDSVRPATLRGLGPDQVLVMVNGKRRHVAALVNVNGTVGRGSAGVDLNAIPA
                     SAIDSVEILRDGAAAQYGSDAIAGVLNLVLKSDAQPLKLEVKGGSTTHSDGQMLDSNL
                     SGGWNVGRGAVFATVEYRDRNQTNRALNDPRDQIVKGDAGNNSVPQPNTHWGDAYERD
                     LMSLVNLNLPISQDAKQVLYAFGTYDLRHGSAGGNYRRALDATDWPQIYPLGFLPLIE
                     PRIKDQALTGGARGEVGSWFYDASAGYGRNKMDF"
BASE COUNT         1850 a         3120 c         3069 g         2085 t
ORIGIN      
        1 ccggcgttcg gatagagatc gatccagcgg tcgttgaaag cgcgctcgat cacgctggtg
       61 tagccggtgc cgaggggcgc gagggcgccg agaatgttct tcttcgacgc atcgagcgag
      121 tactcgagct tcgcattggc gacgagcggc gcgtagaggt cgtagtagtg cagggcattg
      181 atgccgagga ttcgcttgcg aagtttgaga tagcggtgga atgtcggcag gttcgcgttg
      241 acgccttcga tcagctttgt gtagaccgag gtggggatgg tgtcggcgtc gagcgcggcg
      301 tcgagggtgg tcggatactt ccgcaccttc atgtagaaga ggtctttctg aacctcggca
      361 ttcagagcgg tgccgatcgt gccgcgatag tcgccgagcg cgccgaagaa cgcgtcgaac
      421 actttcttgc ggtcctcgcg attggggagc gtacgagaca ccgcgaacgt ggccagatcc
      481 agcttgcgcg tggttccgtc ggaaagggtg accgtgggga aggggaagtc ggcgttcgta
      541 aacacgccgt agacctcggc tggtgcggac gacatcaagc cggcgtccgc gatgatcttc
      601 tcttccggct ccgacagcgt gtgcgcctgc cgccgcagca gatcgtgcag atagaaggtg
      661 taggggacga gcttcggttc gcgagcgatg aacgcatcga tcgtggcgcg atccattttc
      721 agaatctcgg gctcgacgaa tgacttctcg gcgtcgaatg atgattcgag ctgatccatc
      781 tcctgcttca tggcccggta cttcgcgacg cgcgtgtcct gatcagagct gaggcccgcg
      841 tagatggcga tgcgcgtgaa gtctttgttc agatcgctga ccatctccag cccggcggcg
      901 agttgctgcg gcgacgtgcc gagagtccct ttgaacttct tcacctcggg gatgcgagcc
      961 gcgagtttgt ccttcgctac ccgccaggca tcgtcgctgg catagaggtc cgtgatcttc
     1021 cacttcgacg actcggggat gtccgcccgc tctttcgact gcgcgaagcc ggcgctggtt
     1081 gccagcaaaa gaacgaacac cgacaccgca actctcatca tccgcatgaa cacacctcga
     1141 tcctgatttg tcgctgttgc tacggatgct gatcgtactg gtttcgtctg aggagtgccc
     1201 gcggggtgtg ccgagcatgt tcgatggcta ggagctgaaa gtcctttgcc caggttgagg
     1261 aaccgaaggg ccagttagca ccttctggat tcgcgaaaga ctcggacgtc gggagtgagg
     1321 agttgggact cccgacgcta cacccgcacg cgtacgttgc tgactcgttc accctggatg
     1381 ctgtcatgtt catccgacat gcggtcgcgt ttatcggccg aaaaacgcgt ttttcgggcg
     1441 aaaatcgtgt tcatccggcg tgcggtcgcg tttatcagcc gaaaaacacg tttttcaggc
     1501 gaaaatcgcg ttcatccggc gtgcggtcgc gtttatcagc cgaaaaacgc gtttatccac
     1561 cgaaaatcgc gttcatccga cgaaaatcgc gtttttcggg cgaaaatcgc gtttttccgc
     1621 tgaaaaacgc gtttatccgt gtcacctaaa agagacagcg ggataggtcc agctcagtcg
     1681 aggctccccc accccgaatc gaagaacgac cgcagtccta tttcggagtg gcgatgaacc
     1741 actggttgtc gaacgggtcg atgacgaatg ctgctcgttc gccgtacggc ttgtctgccg
     1801 gtgcctccac cgacctcgcg cccgcgcgga cggcgcgctc gtacgtggcg tcggcatcgg
     1861 gaacgtagag atgaatcacc gccggtatcg gctgcgattc gccatgcgcc tcgccgagct
     1921 cgatcattga gtcgccgatg cggacgccgg cgtgtgcgac ctcgcggccg ggcatctcgg
     1981 tcacgtgctc gaccttcgcg ccgaacgcat tttcgaggaa ggtgatcagg tttcgcgcgc
     2041 cgcgcgcatg cacgaacggc gtgaccgtgt gatatcccgc cgggcgcgga ccaacgcccg
     2101 cctctttgcg cggcttgccg cccgcgccgg caaagcgtag ttgcagttct tcctccgtca
     2161 cgttctcctg atgcgttgcg aagtaccaga cgttgcccca gcgatccttc actcctccct
     2221 cgcggtcgcc gtatgcctga tcggtcagcg ggtgcgtcga ggtcgctccg gcggcgagcg
     2281 cccggttgta ggcgccgtcg acgtcgtcga gataaatgtg aagcgccatc ggtctggcct
     2341 tgtattcgcc agccggttcg cccatctcga tgagcgaatc ccccacccgc agcgccgcat
     2401 gcatcactcg ccccgcggca tccgggtagc gtcccagcag ctcggcgttc agcgtttcct
     2461 gcaaaaaagc gatgagtcca tcggcgtcgg gtgcaagcag atagggtctg atcgacggga
     2521 actcagtagt cacttcttcg gccgtcatcg tgctcatgtc atcctccatc ggcggcatca
     2581 gctccgccct cagtttcgat cggaagtccg ggtcagggag accgtgcaga tcgcccgcga
     2641 tcatgagcag cgtcgccagc tcgggctcgg ccagcgcgag tccttcccgg cggccagcca
     2701 ggatggcctc aatcgcgtcg tcgagccgtt cgatcaggtc gcgctcagcc atggttttcg
     2761 cccatctgtg cccgcaacga ctgcaggcct ctccattgaa gttgtttgac cgcgccttcg
     2821 cttttgccga tcgccgaggc gacttcgcga atgctgcgct cctcgccgaa acgcatcacg
     2881 agaacgcgtc gctgatcggc cggtaatcga tcgacacagc ggtatagcga tgcccgtcgt
     2941 tcgatgtgct cgatgtcgtg cggcgcttcg ctttcgacaa gacgctcatc tggactctgt
     3001 tcccgcgcgg tgctgttgaa atggtcgttc acggcgttcg ctgcgattcg atacagccac
     3061 gcgacgaacg gcacatcgcg aatctcgtag ctcttcaggt tcttcaacgc cttgtgaaac
     3121 acctccgacg tcacatcctg cgcggcggcc cgctcgcgca cgcgccggat cacgaacgcc
     3181 caaacccggt tgaagtgggt ctcgtacaac tccgcgaacc ggctgggatc agccttcgct
     3241 gcctcgatca gcagccgctc gcggtcatag gtttcgcgat ccatgaatcg ctcattaaac
     3301 cgccgggcag gcgaaaagtt acggtgattg cgctacattc tttcgatgca acgacgattg
     3361 ctgattgtgc ttctgctttt gatcccggca ggctcagcgt tctctcaagc tgcgcctgag
     3421 aagacgatgc cttcgtggat ggctggcgcg tgggcgtccg attacgatgg tgttcgcatg
     3481 gaggagcact ggacccaagg cgacggtgga ttgatggtcg gtatgcatca ggacctgaca
     3541 gccgatggca agagttcgtt cgagttcctc cgaatcgagc gccgtgacgg caagctcgtc
     3601 tacctggcaa tgccgcacgc ccgtcccgag acgccattcc cggccaaatc cgtcgaggca
     3661 acccgtatcg ttttcgagaa tccggatcac gactttccgc agcggatcat ctactggcgt
     3721 gacggcgcgc ggctctgcgc acgcaccgag ggagcgatca agtcgaagac ggtttcggaa
     3781 gagtggtgct ggcgccggat gacaccgtga gcggagagcg gaccgcgccc tccgaccccc
     3841 gacacccgac acccgacacc ctccgtcgca tcgacacccc attcgccccc gcggtgctga
     3901 tcgcgatcgc ctacacggtc ttcttcctcc ttctcctgca tgcgcgccac gacgacgtct
     3961 ccctcttcgt cgtcgccggc ggcgtaaacg tcgacgcgcg gaaagtgccg ccggatctga
     4021 ccgtcatccc gaatatcggc ggatacgacg gcatcacgtt ctatcgcttc gcgctcgatc
     4081 ccttcaccgc cgcgcggacc gagttcggca tcacgctcga cacgcctcct taccgcaacc
     4141 agcgcatcgc ttatccgttc atcgtctggc tgctctcgct cggccacgcg cgctgggtcc
     4201 cggcgctgct tgtgatcgtg aacatcgccg cagcggcgat catggccgcg ttcggcggtg
     4261 ccttctcccg ccgcctcggt taccacgcct tgtggggcgt catcctgccg ctctatcccg
     4321 gattcctgct gacgctctcg cgcgatctcg ccgagatcgt ggcctcgatg tttgcgatgg
     4381 gagcgatctg ggcgatcagc gcgcggcgca acctcaccgc ggcgattctg ctgacgtgcg
     4441 cggcgctcac gcgcgagccg acgctcctgc tcgcgttcgc gctggccggc gcgtgggtga
     4501 tcgagcggct gctgcgccgc gatcggagga ttgcgcccgt cgttttcctc gcgccgatcg
     4561 tcatcgatgt cctttggcag gtctttctca ccgcgcgctg gggcacttcg ccgcttcgct
     4621 ccggcgcgcc gaccatggcg ttaccgttcg tcgagttcgc ccgcttcttc gccgcatccg
     4681 tcccacgccg cattcaacag cagcggctct acttcctcga atgcctcctc ctcgtttcga
     4741 ccgtcctaac cgtcgtgttg atgtggcgcc ggacgcgcac accgctcgaa tggcgcttcg
     4801 cgtggctcgg ctatctcgcg ctggcgtccg tcctgccgca cacgatctgg ctggaggact
     4861 acggcttcct gcgcatcttc tcggacctct tcgtcgtcag cgccgccatc atcatcgctt
     4921 cgacaaacgg agcccgctgg ttcgcactga tcaccgctgg aggcgtgtgg ctttacctcg
     4981 ctcggatcct ggtaaagctg ggctgatggt cggggttcca ggaatcaacc aggagtggcg
     5041 cggagcgccc tctccgacgc ccgactcccg aaacccgacc cccgctcaac cgccgtcact
     5101 tcgtggcgta ttggtccgcg cccatgaagt gaagggagat gtactgctcc tctcccacga
     5161 cccagctgtc gtgcggcacg gtcggaacgt gaaagagatc ccctgcatgc agttcgatga
     5221 cacgtccgtc gtcgaacgcg accgtggcga tcccggccag gaccattccc acgtgcccaa
     5281 cgttgcagcg cgtcgtgccg acggctggtc caacgtgatc gctccaccgc cagcccggct
     5341 cgtacgtcgc ccggccaatc gtcatggcgc cgagatgaac gacctcgaac tttcctttga
     5401 caaggaaccg cgtctcatcg ggattctcga aacgcttgag gatgacggtg atgtcttcgg
     5461 tattcatgag aaggagggac tgccctctac ttgtactgct ccttcagcgc atcgagctcg
     5521 gcgacggcct tccgcagcac atgaacgacc ttgtgcacct cggtgccgta gaccttcagc
     5581 tcggcgtcgc agtcggacat cgcgtagacc gcttcatcct tctcgaggcg ctcgcgctcg
     5641 aagcgcagac gctcgatcag ctcctcgatc tcgctgacga ggacgaagtt gaggtccttc
     5701 tcgccgaggt aacgcttgca ctgggggcag tagatggagc ggtcgtcggt gttctcgacg
     5761 agctcgaaaa caaactgccg ctggcactgc ggacactcga aaacctcggg taacacgatc
     5821 gcatcctcgc ctgcgaattg tcgcacgaaa ccggaagggg ccagtctgcc gggaccagga
     5881 atcgagtcct cgtgccgccg gaatgcggac gttgaccatg gcgttcaccg ccgtgtagca
     5941 tccacgcccg ccattctggg agatcgttca atggtaggac aagcggctct gaaccgctca
     6001 atcggggttc gactccctgt ctcccagcca ttcaggatac gcagccattt ttcgccagaa
     6061 catcgaaggg caatctcatt gtgactgcga gacttccgcg gctcatcgtg cggttcgata
     6121 gcctttcagg cagaagcgag aaggtgcttc gacgacctcg gcggctgtta gcagctcacg
     6181 attgatctcg tcgagttcct cttcccagcg aaccgtgtgt tcaagccacg ggctctcgtc
     6241 gagccgcccg aggagctttt cttcgtagct cgcggccttg aggcgtttga gttccgccgc
     6301 ttccgcaggc gatccatcgc tgcggtgttg ctgtttgccg acgagtcgta ccgcgcggcc
     6361 agctcgttct ctccgacgga ctcgggattg agatcgtctt caacattgcc gtcgcgttct
     6421 cgtcgagaat gcgctccggg tagttttttc cgttgcacgc tagtagacgt acttgccttt
     6481 cttgatgtcg ccgacgagca agccgttgca agcggcgcag gttgacgaga cccgagtagg
     6541 tgaacgacgc gtgcgcgtct tcggccgcgt gcggccggcg agtacgtcct gcacgtcatc
     6601 gaagagctgc ttcgagatca gcggctcgta cttgccttaa tacgttttcc accccaaacg
     6661 gacgtgccgg cgtagatcga gttcatcagg atcgtgtaaa tcgaggattg gatctctttg
     6721 gaccatgcaa ggcgcaggca cgaccgcggt cggtgcatca ctaacgcggg gcacgcacac
     6781 ccttcacatg ggttggatcg tgacgaaaac cgactccggc agcgggctat cgaccgtgat
     6841 ctcgacgtat ccatcgaact ttgtcgaatt gacgccgatt gaccccacca catcgtagtc
     6901 acccggatac aggtctggcg ggacgacgat cacagtggtg ccgggtccgg gcgcgaaggt
     6961 gaacgtaaac gtggcattca cgttccagta tgcagcccgt gtgatcggtc caaatgtttg
     7021 tgaaggtcgc gtagtcccga aagaaatcgg ggtgccggaa atcaggcggg gatagacgcg
     7081 tgtgaggata tcgagcatcg tctgattgag attcggcggc tcgagtggct gcgacatgtc
     7141 ctgcacgacg cccatcgctg ccgcatcgac gttgttgaaa aagtggtact gcgcattgtt
     7201 gaaccacgcc cagaaaatcg cagacagcat gttttgatcg ttgagatccg caacaaactg
     7261 aatctccgga taattggtgc tgccgccaaa ttcactgcag agcagaggag ggggaacctc
     7321 ccaggacgcc tggtactttt gcgcgttact gattggggcg acaaaaggag tggcagaggt
     7381 gttctcgtag ttgtggaaat tgaatcccag gtttcccacg gaggtcagat caggaagcga
     7441 tgtcggcgcg ttgtagtcga aatagatgtt aggttcaaac cagatcatcg cgccgggatg
     7501 gccgttttga agagccggaa ttgcgcactg ttgatagaaa gcgccgaggt attccggatc
     7561 aaatttggga taaccattgg aaaaattcaa agggttctgg tcgttacagg gtggaacatg
     7621 gtttgggtcg ttacagggtg gaacataccc ttccgtccaa tacgttccgg gctcgggctc
     7681 gttgatggga tcgtagccga ggatgttccc ctgctgatct ttgagatagc ccgaaacaaa
     7741 gttcagcatg tgtccgtagg ccttccacag cgtggtggtg ccatccaaaa tgggggcgtc
     7801 ggcccagaag aaatcaaagg cagaattgag atcggtctgg atatttgtcc gctcgccgtt
     7861 caatacgaac gtgtcgcctc caaaggtgtt caccatccag ccataaatgg gcatggtcgt
     7921 tccttcggtg acgatggccc atgacggcgc gccaaatccc cacggcgcgg cgtaaccgtc
     7981 ctggtggaaa tcgacgagag tgtagatgcc gaagccggct agaagctcga tggttccttt
     8041 gatgctttca aggaaggtca tgttgtattg cagcgaccga gtcgtctcgt caacgtactg
     8101 gacattcgtc cacggcaagc caagtctcac aaccgatacg ccacaccgtt ggagaaaagc
     8161 cgcattgttt tcgaggaagc ccagttgttc tggactctgc gcggtcttcg agcagaggtt
     8221 gaatccgcgc aaaatgacga ccctgccgtc gggatcagtg aggtagtttc cggtagcctt
     8281 cagctgaggg agcggcgtcg ggatttgcgt catgactcaa gtgtaccgca gtagccttgg
     8341 gtcgcggggg tgatggctca tgtctaacgg atcactgcga cgccgagacg atcattgaaa
     8401 gaaccgatca ttgcgcccga ggtcgaggtg aaagaagcgg acctgattct ggcgccggag
     8461 gtcgatgcga tcagcaacga tcgcgaggtc ctggcgacgc cgcggccgac gatcgaccgc
     8521 gtcgtgcccg agaagcatct gcgcgaaacg ctgcgcctcc tgcacgtcga cctcgagaac
     8581 aagagcgagg ctcacggagc gagcacggca gttcgaactt cgtccagtac tcccagccag
     8641 cggcgacgcg ccgcagcgta ggttggccca gcttcggcca ccactccttc aagtagccgg
     8701 cattcgcggc acggcggttc gatactccgc cagtgcccgc agccagcgcc gcgttagcgt
     8761 cgccgaagac ccgtctccca cgcgcggcgg cggcctgtac cggcggcagg ctatactcga
     8821 attctcaata cataaggagc gcgtcatggc tatgagattc cgcctcgcca gtctggtggt
     8881 cctgttcttt tcgctcgtcc tccacgcgca gcagggcacg acggtgcgtg gacgcgtgac
     8941 gatcgcgtcg gacggcgcgc cgctgccggg cgtcacggtc tcgatcaacg agctgaatct
     9001 cagtacggtc accgatgcgg aggggaggta tcagatcccc gtcccatccg cccataccgg
     9061 tcagactgta aaagtcagcg cagccttgca gggctttcag acacacacag ccaccgccac
     9121 tctgggtgcc gccgaggtca cgctcgattt tccgttgcgc gtttctttcg gacaggagat
     9181 caccgtgggg tcgcgcgccg tgaacgcaga ggccgagaaa gcggtgccgg tcgacatcat
     9241 cacgcacgcc cagatcgagt cggcgccgtc gacggaaacg aatcagatca tccaaaaggt
     9301 cgcgccttcg ttcaacttcc cgcggccgac gctctccgat ggcacggaca gcgtccgccc
     9361 ggcgaccttg cgcggcctcg gtcccgatca ggtgctggtg atggtgaacg gcaaacgccg
     9421 gcacgtcgcc gcgctcgtga acgtcaatgg caccgtcggt cgcggttctg ccggtgtcga
     9481 tctcaacgcc attcccgcct ccgccatcga cagcgtggag attcttcgag acggcgcggc
     9541 cgcccagtac ggatcggacg ccatcgccgg tgtgctcaat ctggtgttga agtccgacgc
     9601 tcagcctctg aagctggaag tcaaaggcgg ctcgaccact cacagcgacg gtcagatgct
     9661 ggactcgaat ctcagcggcg gatggaacgt cggccgcggg gcagtgttcg ccaccgtcga
     9721 gtatcgcgac cggaaccaga cgaatcgggc gctcaacgat ccacgcgatc agatcgtcaa
     9781 gggcgatgcc ggcaacaact ccgtgcccca gccgaacact cattggggtg acgcatacga
     9841 gcgcgacctc atgtcgctgg tgaacctgaa tctccccatc agtcaggacg ccaaacaggt
     9901 cttgtatgcg tttggcacct acgatctgcg gcatggatcg gcgggtggga actatcgccg
     9961 cgcgctcgat gccacggatt ggccgcagat ttatcctctt ggcttcttgc cgctgatcga
    10021 gccgcgcatc aaagatcagg ccctgactgg aggcgcgcgg ggggaggttg gctcctggtt
    10081 ctatgacgcc agcgccggct acggccgcaa taagatggac ttct
//
LOCUS       DATLVW010000872         5982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_325572, whole genome shotgun sequence.
ACCESSION   DATLVW010000872 DATLVW010000000
VERSION     DATLVW010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325572"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VNN25_25660"
     CDS             complement(<1..292)
                     /locus_tag="VNN25_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94986.1"
                     /translation="MLTSVARGLALFLGGFTLLNLAGDSRFAGASANSWWISVPLLPP
                     LASKVFLALLGLVLLAYAAAPGVGVQRRVIATALLSAAVLAAVSNSIGFYLML"
     gene            complement(312..1283)
                     /locus_tag="VNN25_25665"
     CDS             complement(312..1283)
                     /locus_tag="VNN25_25665"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HXH94987.1"
                     /translation="MPFEEVKKIWMNGRLVDFADAKIHVLTHALHYGSGLFEGIRCYD
                     TRNQGPAIFRLDDHIRRLYDSCRIYRMEIPYTPDEFKKVCVDVVAANGFEDCYLRPIV
                     YRGFHSLGVFPAACPVDIVVAAWHWGKYLGPEAIEQGVDVRVSSWNRMAPNTFPALAK
                     ATGNYMNSILIRQEAAADGYAEGIALDVNGYVSEGSGENVFVVRDGKIMTPPLGASIL
                     AGITRASIIKMARDLGFDVVESLIPRELLYVADEVFFTGTAAEVTPIRSIDRVPVGNG
                     RRGPVTERIQKEFFDYIAGDIPDRYGWFTPVYAERSAAVPKPALVAG"
     gene            complement(1449..2333)
                     /gene="cyoE"
                     /locus_tag="VNN25_25670"
     CDS             complement(1449..2333)
                     /gene="cyoE"
                     /locus_tag="VNN25_25670"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXH94988.1"
                     /translation="MSDVLALPRPAGFASMTRDYLELSKSRIVMMVLITTAAGYFFAA
                     PRVDWALLLNALVGTALVAAGTNALNQYVEREHDAKMRRTRLRPLPDGRISPRAALFF
                     SVSISIIGTLYLGVAVNWLTAGLGALTLLTYIFIYTPLKRVSTICTIIGAVPGAIPPL
                     MGWTAATGSLGTGGGIVFAILFFWQLPHFMAISWMYREDYARGGFAMLAVQDDEGRVT
                     ARHAVFYSFALLTVSVLPPLFGLATYVYLVGAIAAGGALTVASFFFLADRTPRSARRL
                     FMASNIYLIVAMLLLVLA"
     gene            complement(2330..3313)
                     /locus_tag="VNN25_25675"
     CDS             complement(2330..3313)
                     /locus_tag="VNN25_25675"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXH94989.1"
                     /translation="MKPSFEAAPEALRRSDETPVWLRRYTKLVAGSTLFLIFAGAMVT
                     STGSGLAVPDWPLSYGMVMPPMVGGIFYEHGHRMIAATVGLLTVIQAIWLQLREPKRF
                     TRILGWCSVGAVIAQGVLGGVTVLLLLPHSVSIAHAALAEIFFCLNVSIAFFTSTLFR
                     SSRALEKGDAPIGLTSLLVVVTYAQILVGALMRHLGAGLAIPDFPLSFGRVVPSFTSL
                     EVGVNFAHRVGAICVATLVIGTAVRLRRFESGHPLRQLANLLVTVVMIQIVLGGYVVW
                     SGKQPLMTSVHVMTGAATLALSLLTALTARTVGWRRRRQHTSALLASEVPA"
     gene            complement(3318..4097)
                     /locus_tag="VNN25_25680"
     CDS             complement(3318..4097)
                     /locus_tag="VNN25_25680"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94990.1"
                     /translation="MEKFLAQFMPVDASEHGPALDRLSANVHWLMLVLFVAWGLYFLF
                     VLWRFNAKRNAKASYHGVQSHFSSYAEAGVAVVEVILLVGFSIPMWSAWTHPPSAASN
                     PLEIRVVAEQFAWNVQYPGPDGIFGRRDVKFVTSSNPLGIDLTDPHAKDDIVTLNDLH
                     LEVNRPVIIHVTSKDVIHSFKLPVMRVETDAIPGMEIAIHFTPKMANESGTENDKWEI
                     ACAQLCGLGHYRMRGQMFVHSKNSFQKWLAAAPPAQTSIGS"
     gene            complement(4122..4385)
                     /locus_tag="VNN25_25685"
     CDS             complement(4122..4385)
                     /locus_tag="VNN25_25685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94991.1"
                     /translation="MRRNSQLFMITAVILLVPAVLSACPVCYGNPDSPMTKSSNNAIL
                     FMIGVIGFVQIGFIAMFWSFWRRARAMRRLRESFRLVDGGAAH"
     gene            complement(4382..4582)
                     /locus_tag="VNN25_25690"
     CDS             complement(4382..4582)
                     /locus_tag="VNN25_25690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94992.1"
                     /translation="MSLRVFHIVFISVSVALTLYLAVWGVREFMASRSTLGIGMAVFG
                     IAGGASLVWYGKRVFHKLKELP"
     gene            complement(4583..4960)
                     /locus_tag="VNN25_25695"
     CDS             complement(4583..4960)
                     /locus_tag="VNN25_25695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HXH94993.1"
                     /translation="MTDPAAAVEPGVDVHEHAGIGDPNAHHHSPEHIRKEMRVYLTVF
                     IGLAVLTAVTVGACYGLKLPVRTAIIVALIIASIKGFLVAGFFMHLLSEKKVIYGVLI
                     LTVVFFIFLMTLPISHHANPFGS"
     gene            complement(4973..5596)
                     /locus_tag="VNN25_25700"
     CDS             complement(4973..5596)
                     /locus_tag="VNN25_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXH94994.1"
                     /translation="MDAHASVGHAELEIPYNVHPRPDTGVYNAKLGIWLFLASEVMLF
                     GALFSSLILLRTGAESWPRGYEMLNVPLATFNTFILILSSVTVVMAWSHLRLGNLFKG
                     RVYISITILCALIFLVNKGFEYSHEFREGRFPKTNNFLAIYFTMTGLHGLHILGGVIV
                     FLYFLGPGAKMWAHDPERYTNRIEVVGLYWHFVDLVWIFLFPILYLM"
     gene            complement(5596..>5982)
                     /locus_tag="VNN25_25705"
     CDS             complement(5596..>5982)
                     /locus_tag="VNN25_25705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH94995.1"
                     /translation="GGMQYQHAKQIAAQTNPFMSICAIGLLAAQVPFIFNFFYNAIAA
                     AFGNRDEGLGSNPWGATTMEWAATTSPPLAHGNFEVLPVVYRGPYEYSVPGSEVDFIP
                     QHQPAVIEPAPRGGLLPEKAVAMREN"
BASE COUNT         1310 a         1783 c         1879 g         1010 t
ORIGIN      
        1 cgagcatgag gtagaagccg atactgttgc tcaccgccgc cagaaccgcc gcgctcagaa
       61 gggcggtggc aatcacgcgc cgctgcactc cgacgccggg agcagccgcg tacgcgagga
      121 ggacgaggcc gagcagagcg agaaacactt tgctcgccaa cggcggaagc agaggcacgc
      181 tgatccacca gctattcgca ctcgcgccgg cgaatcggga atcgccggcc agattcaaca
      241 gggtgaatcc tcccagaaac agcgccagtc cacgcgccac ggaagtcagc atcaggaact
      301 gacggcgctt cctaccccgc caccagcgca ggtttcggaa ccgctgccga tcgctccgcg
      361 tacaccggcg tgaaccagcc gtaccggtcg gggatgtcgc cggcgatgta gtcgaagaac
      421 tccttctgaa tgcgttcggt gaccgggccg cggcgtccgt tgccgacggg gacgcggtcg
      481 atgctgcgga tcggcgtcac ctcggcagca gtgccggtga agaagacttc gtcggcaaca
      541 tagagcagtt cgcgcggaat cagcgactcg accacatcga agcctaggtc gcgcgccatc
      601 ttgatgatgc tggcccgcgt gattcccgcc aggatcgatg cgccaagcgg cggcgtcatg
      661 atcttgccgt cgcgaacaac gaatacgttc tcgcccgagc cttcgctcac gtagccgttg
      721 acgtcgagcg cgatcccctc ggcgtaacca tccgcggccg cttcctgacg gatcaggatc
      781 gagttcatgt agttgccggt ggctttggcc aatgctggga acgtgttcgg cgccatgcgg
      841 ttccacgacg acacacgcac gtcgacgccc tgctcgatgg cctcggggcc aaggtacttg
      901 ccccagtgcc aggcggcgac gacgatgtcg acagggcagg ccgcggggaa aacacccagc
      961 gagtggaatc cgcggtagac gattggacgg aggtaacagt cttcgaagcc attggcggcg
     1021 acgacgtcga cgcagacctt cttgaactcg tccggcgtgt agggaatctc catcctgtaa
     1081 atgcggcaac tgtcgtacag ccgccggatg tggtcgtcga gccggaaaat cgctggtccc
     1141 tggttgcgcg tgtcgtagca gcggatgccc tcgaacagcc ccgaaccgta gtggagggca
     1201 tgcgtgagca cgtgaatctt ggcgtcggca aagtcgacga ggcgcccgtt catccagatt
     1261 ttcttcactt cttcgaacgg catcggagcc tcctgaatcg gtggcagcgg tggacgggca
     1321 gcagcggcgc gcctaggctg gaaagctgcc ggagtatagg ttccggcgtt tcagcaagtc
     1381 aatgcgacaa ctctgacttt ttggcggcgc gcacacgaaa gaccggaccg caatttgacc
     1441 agcgcactct acgccagtac gagcagcagc atggccacga tcaggtaaat attcgatgcc
     1501 atgaagagcc gccgcgcgct gcggggagtg cggtccgcga ggaagaagaa gctggcgacg
     1561 gtgagcgctc ctccggccgc gattgcgccg accaggtaga cgtacgtcgc cagtccaaac
     1621 agaggaggca gcacgctgac cgtgagcagg gcgaagctgt aaaacacggc atgccgggca
     1681 gttacgcgcc cttcgtcatc ctgaacagcc agcatggcga atccgcctcg ggcgtagtcc
     1741 tcgcgataca tccaactgat ggccatgaag tgcggcaact gccagaaaaa aaggatggcg
     1801 aagacgatcc ctccacccgt tccgagcgag ccggtcgcag ccgtccatcc catcaacggc
     1861 ggaatcgcgc ccggcactgc gccgatgatc gtgcagatcg tggagactcg cttcagggga
     1921 gtgtagatga agatgtaggt cagcagagtc agcgcgccga ggcctgccgt gagccagttt
     1981 acggccacgc cgagatagag cgtgccgatg atcgagatcg agaccgagaa gaacagcgcc
     2041 gcgcgtggtg agatccgtcc gtcgggcaac gggcgaagac gcgtgcggcg catcttcgca
     2101 tcgtgctccc gctccacgta ctgattgagc gcgttcgtcc ctgccgcgac gagcgctgtt
     2161 ccgacgagcg cgttgaggag caacgcccaa tcgacacgcg gcgccgcgaa gaaatagccg
     2221 gctgccgttg tgatgagaac catcatcacg atgcgcgatt tcgacagctc gaggtagtcg
     2281 cgagtcatgc tggcgaatcc ggccggccgc ggcaacgcga gaacatcgct catgcaggca
     2341 cctcggatgc gagaagcgca ctcgtatgct ggcggcgcct ccgccagccg accgtgcgtg
     2401 cggtcaacgc ggtcagcagg gagagcgcca acgtcgcggc gccggtcatc acgtgcacgc
     2461 tggtcatcaa gggctgcttg ccgctccaga ccacgtagcc gccgaggaca atctggatca
     2521 tgactaccgt cacgaggagg ttggcaagct gccgcaacgg atgtcccgac tcaaagcgac
     2581 gcaggcggac agcagtgccg atcacgaggg ttgcgacgca gatcgctcct acgcgatgcg
     2641 caaagttgac gccgacctcg aggctggtga acgaaggcac gacgcgcccg aacgacagcg
     2701 gaaaatcggg gatggccagg cctgcgccga ggtgacgcat cagcgcgccg acgaggattt
     2761 gcgcataggt cacgaccacg agcagcgaag tcaacccgat cggcgcgtca cccttttcga
     2821 gcgcgcgcga ggaacggaac aaagtcgaag tgaaaaacgc gatcgacacg ttcaggcaga
     2881 agaagatctc ggcgagcgca gcgtgcgcga tcgagacgga atgcggaagg agcagcagca
     2941 cggtcacgcc gccaaggacg ccttgggcga tgacggcgcc gactgagcac cagccaagga
     3001 tgcgcgtgaa acgcttcggc tcgcgcagtt gcagccagat cgcctgaatc actgtgagca
     3061 ggccgacggt agccgcgatc atgcggtgac catgttcgta gaagattccg ccgaccatgg
     3121 gcggcatcac catgccatac gaaaggggcc agtcgggaac ggcgagaccg gagccggtcg
     3181 aggtgaccat cgctcccgcg aaaatgagga acagcgtcga cccggcgacg agcttggtgt
     3241 atctccgcaa ccagaccggg gtctcgtccg aacggcgcag cgcctcgggc gccgcttcga
     3301 aagaaggttt catgtttcta ggagccaata cttgtttgcg ccggcggtgc agcggcgagc
     3361 cacttctgga acgagttctt cgaatggacg aacatctggc cgcgcatccg gtaatgaccg
     3421 agaccgcaaa gctgcgcgca ggcgatttcc cacttgtcgt tttccgttcc tgattcgttg
     3481 gccattttcg gtgtgaagtg gatggcgatc tccatccccg ggatggcatc ggtctccacg
     3541 cgcatcaccg gcagtttgaa gctgtgaatc acgtccttcg aggtgacatg aatgatcacc
     3601 ggacggttga cctcgaggtg gaggtcattg agcgtaacga tatcgtcttt tgcatgcgga
     3661 tcggtgagat cgattccgag cgggttcgat gatgtgacga acttgacgtc gcgccttccg
     3721 aagattccgt ccgggcccgg atactgaacg ttccatgcaa actgctcggc gacgacgcgg
     3781 atttccagcg gattcgatgc tgccgaggga ggatgggtcc aggcagacca catcggaatc
     3841 gaaaaaccga ccagcaggat gacttccacg actgccacgc cagcctcggc gtagctcgag
     3901 aaatggctct gcacgccgtg gtagctcgct ttcgcgttcc gtttcgcgtt gaagcgccac
     3961 aggacgaaga ggaagtagag accccaggcg acaaagagga cgagcatcag ccagtgcacg
     4021 ttcgcactga ggcggtccag cgccgggcca tgctccgagg catcgaccgg catgaactga
     4081 gcgagaaact tttccatagt gcttccctcc agcggcgcgc gttagtgcgc cgctcctccg
     4141 tcaacaagac ggaatgactc ccgtaagcgg cgcatggccc tggcgcgccg ccagaaactc
     4201 cagaacatgg caatgaatcc gatctgcacg aaaccgatga cgccgatcat gaacaagatg
     4261 gcgttgttcg agcttttcgt catcggtgag tccggattgc cgtagcagac cggacacgcg
     4321 gacaaaacgg ccggcaccag cagaatcacc gccgtgatca tgaagagctg actgttgcgt
     4381 ctcatggcaa ctccttcagc ttgtgaaata cgcgttttcc gtaccacacc agcgaggcgc
     4441 cgccagcgat gccgaatacg gccattccga ttcccagcgt gctccgtgag gccatgaatt
     4501 cgcgcacacc ccacaccgcg aggtacagcg tcagggcaac cgagacgctg atgaacacga
     4561 tatggaagac gcgcagggac atttacgatc cgaatggatt ggcgtggtga ctgatcggaa
     4621 gcgtcatcag gaagatgaag aagacgaccg tcaggatcag cacgccataa atgactttct
     4681 tttcagacag aaggtgcatg aaaaagccgg cgacaaggaa acctttgatc gacgcgatga
     4741 tgagcgccac gatgattgcc gtacgaaccg gaagctttaa cccgtagcac gcgcccaccg
     4801 tcaccgcggt gaggacggca agcccgatga agaccgttag atagacgcgc atttccttgc
     4861 ggatgtgctc cgggctgtga tggtgcgcgt tgggatcgcc aatgcctgcg tgctcgtgca
     4921 cgtcgacgcc tggctccact gcggctgctg gatcggtcac gtcgtgctcc tcctacatga
     4981 ggtacagaat cgggaaaagg aaaatccaga cgaggtcgac gaaatgccag tagaggccga
     5041 caacctcgat ccgattggta tagcgctccg ggtcgtgcgc ccacattttc gcgcccggtc
     5101 ccaggaaata gaggaagacg atcacgccgc caaggatgtg cagtccgtgc agaccggtca
     5161 tcgtgaagta gatggccagg aaattgttcg tcttcgggaa acgtccctcg cggaattcgt
     5221 gggagtactc gaaacccttg ttgacgagga agatgagcgc gcaaaggatc gtaatcgaga
     5281 tatagacgcg gcccttgaat agattgccga ggcgcaggtg agaccaggcc atgacaacgg
     5341 tcacgctcga cagaatgaga atgaacgtat tgaacgtcgc gagcggcacg ttcagcattt
     5401 cgtagccgcg cggccaggac tcggcacccg tccgcagcag gatcagcgac gaaaacaacg
     5461 cgccgaacaa catgacttcc gaagcgagaa acagccagat tccgagcttg gcgttgtaaa
     5521 caccggtatc cggccgtgga tggacgttgt acggaatctc gagttctgcg tgaccgaccg
     5581 aagcgtgcgc gtccattaat tctccctcat cgctacggct ttttcgggca gcagaccacc
     5641 acgcggcgcc ggctcaatca cggccggttg atgctgcgga atgaaatcca cctcggagcc
     5701 gggaacgctg tactcatacg gcccgcgata gacgaccggc aggacttcga aattgccgtg
     5761 tgccagcgga ggcgacgtcg tcgctgccca ctccatcgtc gtcgcgcccc acggattcga
     5821 gccgagccct tcgtcgcgat taccgaaagc ggctgcgatc gcgttgtaga agaagttgaa
     5881 gatgaacggc acctgcgcgg cgagcaggcc gattgcgcag atcgacatga acgggttcgt
     5941 ttgcgccgcg atctgcttcg catgctggta ctgcattccg cc
//
LOCUS       DATLVW010000873         5833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_162218, whole genome shotgun sequence.
ACCESSION   DATLVW010000873 DATLVW010000000
VERSION     DATLVW010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162218"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /gene="infA"
                     /locus_tag="VNN25_25710"
     CDS             complement(<1..87)
                     /gene="infA"
                     /locus_tag="VNN25_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXH94996.1"
                     /translation="MSKEEAIEVTGTVLETLPNAMFQVELENK"
     gene            complement(166..990)
                     /gene="map"
                     /locus_tag="VNN25_25715"
     CDS             complement(166..990)
                     /gene="map"
                     /locus_tag="VNN25_25715"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXH94997.1"
                     /translation="MLDRMKGPILKNREEIAVMDRANRIVLDILEIMRSMIKPGATTA
                     QLNAVAEEELAKRGAKSPFKGYAPMGLPPYPAVVCSSVNDVIVHGIPNRAKLEEGDIV
                     GLDFGSIFEGFVGDGAITVPVGKVTPRAQELIDTTRECLDLAIDEMRPGRHIGDIGAA
                     VQEHAEHHGFHVIRNFVGHGIGRRMHEDPQVYNYGRRGRGLELRAGMVLAVEPMLCEV
                     GSKLRSRIERAGRDGVIDDVRTDADRWTARTIDGTLAAHFEHSIAITADGPVVLGK"
     gene            complement(993..1655)
                     /locus_tag="VNN25_25720"
     CDS             complement(993..1655)
                     /locus_tag="VNN25_25720"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXH94998.1"
                     /translation="MKLIFIGPPGSGKGTQAKRLAAQHGVPHISTGDMLREAIADGTA
                     LGQQAAPIMASGALVSDDLMIGIINDRLAKPDAARGFILDGFPRTVVQAEKLDTIVGD
                     SKGNGSGNEADGLRVLQLLVPDEAIVKRISLRRSCPVCGAIYHLESAPPAVDSVCDRE
                     GAELIARPDDNETAVRKRLEAFHRQTLPVATFYKTKQVLREVDGIGPVDEVFERIERS
                     LS"
     gene            complement(1668..3077)
                     /gene="secY"
                     /locus_tag="VNN25_25725"
     CDS             complement(1668..3077)
                     /gene="secY"
                     /locus_tag="VNN25_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXH94999.1"
                     /translation="MNFLETLRNIWAIEDLRKRVLFTFALLAVYRLGSHIPTPGVDPV
                     ALAEFFKAMSGGVFGFLDLFSGGALRRLSVFALGIMPYISASIILQLLTVVWPYLEKL
                     SKEGEMGRRKINQYTRYGTVALSIVQSLGVAFWLKSQAAPGGAPLVPVSVQDSLWGIG
                     FPIMTVLTLTTGTSFIMWLGEQISERGIGNGISLIIFAGIVVSLPSAVLGVIDDVRNG
                     QRNLFAMLGLIVFMAAVVAFVVFMERAQRRIPVQYAKRVVGRKVYGGSNTYLPLRVNT
                     AGVIPVIFASSILVIPQTLASMIKTPWAQAIGGQMRVGEPLYYLLQVVGIIFFGYFYT
                     SIVFNPVDTADNMRKYGGFIPGIRPGKKTSDYIDRVLTRITFVGSLYLAAVCILPEFL
                     IAGVHVNQLPFGVGAALDRALPLWFTEGMGFNFYFGGTSLLIVVGVAMDTIQQIESQL
                     VMRHYDGFMKRGRVRGRRG"
     gene            complement(3079..3522)
                     /gene="rplO"
                     /locus_tag="VNN25_25730"
     CDS             complement(3079..3522)
                     /gene="rplO"
                     /locus_tag="VNN25_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXH95000.1"
                     /translation="MELNNLKPKRTSRHSKKRVGRGPGSGNGKTAGRGEKGQKSRAGF
                     SRMRGFEGGQMPLHRRLPKRGFTNIFKKIHAVVNLSDLERFDNGATIDEGALRQAGLV
                     KGRNDGVKVLGHGKLSKKLTVHATKFSETARKQIEAAGGTCQEIQ"
     gene            complement(3613..3822)
                     /gene="rpmD"
                     /locus_tag="VNN25_25735"
     CDS             complement(3613..3822)
                     /gene="rpmD"
                     /locus_tag="VNN25_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXH95001.1"
                     /translation="MSPAAKSSSKKKMIQIKQVRSGIGCPRYMRETLRALGLGKMHRV
                     TSRVDSPETRGMIARIPHLVQVVEQ"
     gene            complement(3819..4286)
                     /gene="rpsE"
                     /locus_tag="VNN25_25740"
     CDS             complement(3819..4286)
                     /gene="rpsE"
                     /locus_tag="VNN25_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXH95002.1"
                     /translation="MIDKVVHINRVTKVVKGGKNFSFSALVVVGDGKGRVGFGSGKAK
                     EVPAAIKKGIEIAKKNMISISMAGTTIPHAVLGHYGAGQVMLKPASEGTGVIAGGPVR
                     AILESAGIQNILTKSLGTANPHNVVKATFDALAQLRSEAQYKTLRGVEEGTEA"
     gene            complement(4368..4727)
                     /gene="rplR"
                     /locus_tag="VNN25_25745"
     CDS             complement(4368..4727)
                     /gene="rplR"
                     /locus_tag="VNN25_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXH95003.1"
                     /translation="MDRAKLRVASRGRIRQRIRNKVSGTEQRPRLAVFKSLKHIYAQV
                     IDDASGNTIISASTREKDAGSKGANAAAAKAVGALIAKKAKDKGITRVVFDRGGYLYH
                     GNIKALADAARENGLEF"
     gene            complement(4850..5392)
                     /gene="rplF"
                     /locus_tag="VNN25_25750"
     CDS             complement(4850..5392)
                     /gene="rplF"
                     /locus_tag="VNN25_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016527006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXH95004.1"
                     /translation="MSRIGRKEIPVPKGVEVKHDGNVVTVKGPKGTLTTAVVPGIGVT
                     IENNVVQFTRSADDGRMRGFHGLMRALVANNVRGVTDGFKRELDIVGVGYRAEVKGKE
                     VVFQLGYSHPIRFAIPAGIEIAVEPKSGHITITGIDKQKVGQTASEIRALREPDPYKG
                     KGIKYSDEVIRRKAGKAAGK"
     gene            complement(5414..5815)
                     /gene="rpsH"
                     /locus_tag="VNN25_25755"
     CDS             complement(5414..5815)
                     /gene="rpsH"
                     /locus_tag="VNN25_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXH95005.1"
                     /translation="MSMTDPIADMLTRIRNGIQSHHERVELPASKLKVEIARILKIEG
                     FIRNYNLAQKDEPQGTLRIELKYAPNGEPVIHGIERISRPGRRVYRNKQEIPSVLGGL
                     GLAIVSTSKGVLSGQEAVKSGVGGEVLCQVW"
BASE COUNT         1021 a         1825 c         1798 g         1189 t
ORIGIN      
        1 cttgttctcc agctcgacct gaaacattgc gttcggcaat gtctccagga cggttccggt
       61 cacttcaatc gcttcttcct tcgacatgtt cctccgttac gagctctcgg ctctcgggtg
      121 ccggctctcg ggaaaccccg acacccgaca cccgacagcc gacgcctact tccccagcac
      181 taccggccca tccgccgtaa tcgcaatcga atgctcgaaa tgcgcggcca gcgtgccgtc
      241 gatcgttctc gcggtccatc gatcggcgtc ggtacgaacg tcgtcgatga ctccatccct
      301 gccggcgcgc tcgattcgcg agcgcagctt cgatccgacc tcgcaaagca tcggctcgac
      361 cgccagcacc atccccgccc gcaactccaa cccacggccg cggcggccgt agttgtaaac
      421 ctgcgggtcc tcatgcatcc ggcgtccgat cccatgtccg acaaaattcc ggatcacgtg
      481 gaagccgtga tgttccgcat gctcctgaac cgccgcgccg atatcgccga tgtgccggcc
      541 gggacgcatc tcgtcgatgg ccaggtcgag acattcgcgc gtcgtatcga tcagttcctg
      601 tgcgcgaggc gtgaccttgc ccaccggaac cgtgatcgcg ccgtcgccga cgaacccttc
      661 gaagatcgag ccgaagtcga gtccgacgat gtcgccttcc tcgagtttgg cgcggttcgg
      721 aatgccgtga acgatcacgt cattgaccga cgagcagacc accgccggat agggcggaag
      781 tcccatcgga gcatagcctt tgaacggcga tttcgcgccg cgcttggcca gctcttcctc
      841 ggcgaccgca ttcaactgcg ccgtcgtcgc gccgggcttg atcatcgacc gcatgatctc
      901 gaggatgtcg agaacgatgc ggttggcgcg atccattacc gcgatctctt cgcggttctt
      961 gaggatcggc cctttcatac gatcgagcat ggtcaggaga gggatctctc tatacgttca
     1021 aagacctcat cgacgggacc gatcccgtcg acttcgcgca aaacctgttt cgtcttgtaa
     1081 aacgtggcca ctggaagcgt ctgacgatga aacgcttcca gccgtttccg aacggcagtc
     1141 tcgttgtcgt ccggacgagc gatcagctcc gcaccttccc ggtcacagac cgagtccacg
     1201 gccggaggag cggattcgag atgatagatg gccccgcaga ccggacacga ccggcgcagc
     1261 gagatccgtt tgacgatggc ctcatccggg accagcagtt gcagaacccg gaggccatcg
     1321 gcctcattcc ctgagccatt tcccttactg tcaccgacga ttgtgtcgag cttctcagcc
     1381 tggacgactg tgcgcgggaa gccatccagg atgaatcctc gcgccgcgtc cggtttcgcc
     1441 agccggtcgt tgatgatgcc gatcatcagg tcgtccgaaa ccagcgctcc agaggccatg
     1501 atcggcgcgg cctgctgccc gagcgcggta ccgtcggcaa tcgcctcgcg aagcatgtcg
     1561 cccgtcgaga tgtggggaac cccatgctgg gcggcaagcc gcttcgcctg ggttcccttt
     1621 ccgcttcccg gcggaccgat aaagatcagc ttcacgagtg tcgatcctta cccgcgccgt
     1681 ccgcggactc ggccgcgctt catgaacccg tcgtagtgac gcatgacgag ctgcgattcg
     1741 atttgctgga tggtatccat ggccacaccg acgacgatca gcagtgaagt accgccgaag
     1801 tagaagttga agcccatgcc ttccgtgaac cacagcggaa gagcgcgatc gagcgcggcg
     1861 ccgactccga acgggagctg gttaacgtgc acgccggcaa tgaggaactc ggggaggatg
     1921 cagacggcag ccaggtagag cgatccgacg aaggtgatgc gcgtcagcac gcggtcgatg
     1981 tagtccgagg tcttcttgcc ggggcggatg cccgggatga agccgccgta cttgcgcatg
     2041 ttgtccgcgg tatcgaccgg attgaagacg atcgacgtgt agaagtagcc gaagaagatg
     2101 atgcctacga cctgcagcag gtaatagagc ggctcaccga cgcgcatctg gccgccgatg
     2161 gcttgcgccc acggtgtttt gatcatcgag gcgagcgtct gcgggatgac caggatggac
     2221 gacgcgaaga tgaccgggat gacgccggca gtgttgacgc gcagcggaag gtacgtgttc
     2281 gagccgccgt agaccttgcg gccgacgacg cgcttggcgt actgcactgg tattcgacgt
     2341 tgcgctcgtt ccatgaagac gacgaacgcg acgacggcgg ccatgaagac gatcaggccc
     2401 agcatggcga agaggttgcg ctgaccgttg cggacgtcgt cgatgacgcc aagcacagcg
     2461 gacggcagac tgacgacgat gccagcgaag atgatcagcg agattccgtt gccgatcccg
     2521 cgctcggaga tctgctcacc gagccacatg atgaacgacg taccggtcgt gagtgtgagc
     2581 acggtcatga tggggaatcc gattccccac agagagtcct gcacggacac cggcacgagc
     2641 ggagcgccgc cgggagcggc ctgcgacttc agccagaaag cgacaccgag cgactggacg
     2701 atcgagagtg ccacggtgcc gtagcgcgtg tactggttga tcttgcggcg ccccatctcg
     2761 ccttctttcg aaagcttttc gaggtacggc cagacgaccg tgaggagctg caggatgatc
     2821 gacgccgaga tgtacggcat gatgccgagg gcgaagacgg agagacggcg cagagcgccg
     2881 ccggagaaga gatcgaggaa gccgaagacc ccgcccgaca tcgccttgaa aaactccgcg
     2941 agcgcgaccg ggtcgacgcc gggcgttggg atgtgcgatc cgaggcggta gacagcaaga
     3001 agcgcaaacg tgaaaagcac acgtttgcgc aggtcttcga ttgcccagat gttgcggagt
     3061 gtctcgagga agttcaagtt actgaatctc ctggcaggtg ccgcctgccg cttcaatctg
     3121 tttgcgagcg gtctcggaga acttggtcgc gtgcacggtc agcttcttgg agagcttgcc
     3181 gtgaccgaga accttgacgc cgtcgttgcg gcctttgacg agtccagcct ggcgaagggc
     3241 tccctcgtca atcgtggcgc cgttatcgaa acgctcgagg tcggagaggt tcacgactgc
     3301 atggattttc ttgaagatat tcgtgaagcc gcgcttcggc agacggcgat gcaacggcat
     3361 ctgtccacct tcgaagccac gcatacgcga aaagccggca cgtgacttct gccccttctc
     3421 gccgcgtccc gcggtcttgc cgttgcccga tcccgggccg cggccgacgc gcttcttcga
     3481 gtggcgcgag gttcgtttcg gtttgaggtt gttgagttcc atgttttctc cggatgtcgg
     3541 gggtcgggta ccggttgccg ggatcattgc ctcccgagag ccggtcgctg accgccgaaa
     3601 gcttccttcg ttctactgct cgaccacctg cacgagatgc gggatacggg cgatcatgcc
     3661 gcgcgtttcc ggcgaatcga ctcgtgacgt tactcggtgc atcttgccaa gtccgagcgc
     3721 gcggagcgtc tcgcgcatgt agcgcgggca gccgatgccg ctgcggacct gtttgatctg
     3781 gatcatcttc tttttgctgc tgcttttggc ggcggggctc atgcttcggt gccctcctcc
     3841 acgccgcgga gcgtcttgta ctgcgcttcg ctgcgaagct gggcgagcgc gtcgaacgtg
     3901 gccttgacga cgttgtgcgg attggcggtg cccagcgact tcgtcaggat gttctggatc
     3961 cctgccgact cgagaatggc ccggaccgga ccaccggcga tgacgcccgt accttccgag
     4021 gccggcttga gcatgacctg accggcgccg tagtggccga gaacggcgtg cgggatcgtg
     4081 gtgcctgcca tggagatcga gatcatgttc ttcttggcga tctcgattcc cttcttgatt
     4141 gcggccggca cttccttcgc cttacccgag ccaaagccga cgcggccctt gccgtcgcca
     4201 acgacgacga gcgcggagaa gctgaagttc ttgccgcctt tgacgacctt ggtgacgcga
     4261 ttgatgtgga cgaccttgtc gatcatcccg ctttcgctgc gctcgtcgcg gcggtcgccg
     4321 cgctgagagc cctgacctcg ttgcatcgga cgctgcacgt tgcctcccta gaattccaga
     4381 ccgttttcgc gcgcggcatc cgcgagcgct ttgatgttgc cgtggtacag atagccgccg
     4441 cggtcaaaca ccacgcgcgt gattcccttg tctttcgcct tcttcgcgat gagcgcaccg
     4501 acagccttgg ccgccgctgc gttggcgccc ttggaaccgg cgtccttctc gcgagtcgaa
     4561 gccgagatga tcgtattgcc gctggcgtcg tcgatcacct gagcatagat gtgcttcagg
     4621 ctcttgaaga cggccagacg cggacgctgt tccgtcccgc tgaccttgtt ccggatccgc
     4681 tgacggatac ggcctcttga tgccactctg agttttgcgc gatccatgat ttttcctttt
     4741 cgccgaggct tggtacccgg ctatgaggga gtggggggtg gggagtacgg ggtggtagct
     4801 gacgatcaag ctcccacacc ccactcccca caccctactc cccgctcacc tatttccctg
     4861 ctgccttacc cgccttgcgg cgaatgactt cgtccgaata cttgatgcct ttgcccttgt
     4921 agggatcggg ctcgcgaaga gcgcggatct ccgacgcggt ctggccgact ttctgtttgt
     4981 cgattccggt gatggtgatg tgaccgctct tcggctccac ggcgatctcg atccctgccg
     5041 gaatggcgaa gcgaatggga tgcgagtagc cgagctggaa gacgacttcc ttccctttga
     5101 cttcggcacg atagccgacg ccgacgatgt cgagctcgcg cttgaagccg tcggtcacgc
     5161 cgcggacgtt gttcgccacg agcgcgcgca tgaggccgtg aaagccgcgc attctcccgt
     5221 catcggccga gcgcgtgaac tgcacgacgt tgttctcgat cgtcacgccg atgcccggca
     5281 cgacggcagt ggtcagggta cctttcggtc ccttcaccgt cacgacgttg ccgtcgtgct
     5341 tcacttcgac gcctttcggc accgggattt cttttcgtcc aattcgtgac atgggaatct
     5401 ccttagatgc tcgctaccag acctggcaga gaacttcgcc gccgacgccg ctcttgacgg
     5461 cttcctgtcc ggacagcacg cctttcgaag tcgagacgat ggccaggccg agcccgccga
     5521 gcacgctcgg gatctcctgt ttgttgcggt agacacggcg gcccgggcgc gagatgcgct
     5581 cgatgccgtg aatgaccggc tcgccgttgg gggcgtactt cagctcgatc cggagcgtcc
     5641 cctgcggctc gtccttctgg gcgaggttgt agttgcggat aaagccctcg atcttgagga
     5701 tgcgggcgat ctccactttc agcttcgacg cgggaagctc gacgcgttcg tgatgcgact
     5761 ggattccgtt gcgaatccgc gtgagcatgt ccgcgattgg gtctgtcatc gacattcgtg
     5821 actcctgtga ttc
//
LOCUS       DATLVW010000874         5431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_489675, whole genome shotgun sequence.
ACCESSION   DATLVW010000874 DATLVW010000000
VERSION     DATLVW010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489675"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VNN25_25760"
     CDS             <1..1284
                     /locus_tag="VNN25_25760"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXH95006.1"
                     /translation="VALDTFVRERGAASLLDRTRDYWRLWVKNDVDISRLPEAVQRLY
                     RRSLLIIRTQVDHSGAVIAATDSDIVQFGKDTYTYMWPRDGAIVAMALLQAGYAEITR
                     RFFDFCCRLITPEGFLLHKYNPDGSVGSSWHPWLADGKRQLPIQEDETALVVVALWTH
                     FEKFHDVEFVKPLYKPIIKQMADFLARYREPHTRLPAPSWDLWEERRGIAAFTIGSVW
                     AGLYAAARFTDAFGETAQAEKYRRAAAEIREATSRFLFDETSGCFARSVNVSREGEIT
                     TDPTLDASMAGLWLFGMFDAGDPKIVATMEAMIARLTIKTDIGGVARYENDYYFQVSK
                     DVQNVAGNPWIVCSLSVAHWYAMQAQTTAELQKAIDILRWACDRALPSGVLAEQLDPY
                     TGAPLSVAPLTWSHAAFVLATHVVARRAVELAEKG"
     gene            1287..2474
                     /locus_tag="VNN25_25765"
     CDS             1287..2474
                     /locus_tag="VNN25_25765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH95007.1"
                     /translation="MVRRTRLRSRVWLLTAAFALVLFGITFGLSWRAQVAQDRWSRLV
                     GVETRAIATLEELIRAQNAYRAHAVAGDASRYRIVAQLLESDSLSTIDTAALRRRMET
                     FRSAIADNTSQPDEIDTESLRVVAEAQRMIDERKREIARQLPMLERESRELMTAGLAV
                     AWIVVICSFAAVQVTLRKVVRPLEDLSRAADRIAAGDLGARAQVAGDFEIANLAAAVN
                     HMADELKAHARTDELTNLPNFRAFRERIDLEIERASRYPEHVGILILDLDRFKKYNDT
                     YGHLAGNDALQRVARVIRETVRSVDFPARYGGEEFAVIVPQIDAASLAAIAERVREGI
                     ESMSAPKDGAAVTVSIGASIFPDDGAAVDVLFRVADERLYAAKKAGRNRVVVGSKELR
                     LSS"
     gene            2598..4568
                     /locus_tag="VNN25_25770"
     CDS             2598..4568
                     /locus_tag="VNN25_25770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXH95008.1"
                     /translation="MTIVPARDESVIEFTARAVIAGIFFGILFGAANAYLGLKVGLTV
                     STSIPIAVLTVALFKLMPGRREGVILEANLSQTIGSASSSLATGTIFTIPALFLWGIA
                     PPFWQVSILALLGGFLGIAAMVPLRRLLIVKSAAELPYPEGTACAEVLRATSVGRSGG
                     KWIFIGLAVGGAIKLALGAFMLIPSGLAMHLPVLAKAELALEIAPALLAVGYILGYKP
                     SAIMVSGSLISAIVLTPLIATFGAGLTAPMFPEMKLSIAAMTAAQIWSRYVRYIGAGA
                     VAAAGIVAVIKALPTMASSFAAVMRGLRGGGLQTIDDERTPRTDRDVPSWIVVVVPLA
                     VIITLVAVPGLLAGNMSFVPRLVAAIGVAIFGVCFVVVSSRIVGLIGVSSNPTSAMTL
                     VTLLGASVVFVLCGWTDPSARAAILTVGTVVCIAASKAGDISQDLKTGFIVGATPAKQ
                     QFGQFLGAACACWAIAGTVLLLGKAFTFGSPDLPAPQATLMKTVIEGVLSGSLPWALV
                     GTGSALSICAIIAGLPGLSFAVGIYLPLGTLAPVFVGGIVRRMVEAKRDAKSLDSDPG
                     VLAASGMIAGEGLVGVLIAFLVAASGKFPGLASALASMHFASKDFTWLKGSIAIITGI
                     AIVIAICSLLYRAGRSAPADGSTPNDAGIVRS"
     gene            4586..4858
                     /locus_tag="VNN25_25775"
     CDS             4586..4858
                     /locus_tag="VNN25_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95009.1"
                     /translation="MAKKKRKLTRAEKAAKAVRKATFETIFINGQQKRVRRRNLIEGM
                     TVEDFIRANADPVWLHQNEMWEYMAPEPPDDREESIPDHQGDEPPF"
     gene            4878..5267
                     /locus_tag="VNN25_25780"
     CDS             4878..5267
                     /locus_tag="VNN25_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH95010.1"
                     /translation="MKRTWTVIGVSDVPASFKWYQSLFGQPETQPAHPYWGQILDSDG
                     TVLLCLHQWGAHEHPSLMSPDNATPGNGLLLFFRVDDFDRALIRARGLVARLEEEPHM
                     NPNTETAEFSLRDPDGYYVTISALSAA"
BASE COUNT          939 a         1738 c         1728 g         1026 t
ORIGIN      
        1 gtggcgctcg acaccttcgt gcgcgagcgc ggcgcggcct cgctgctcga tcgcacgcgc
       61 gactattgga ggttgtgggt caagaacgac gtcgacatca gccggttacc cgaggcggtg
      121 caaaggctgt atcgacgctc gctgttgatc atccgcacgc aggtcgatca cagcggcgct
      181 gtaatcgccg cgaccgactc cgacatcgtg cagttcggca aggacacgta cacgtatatg
      241 tggccgcgcg acggcgcgat cgtggccatg gcgctgctgc aggccgggta cgccgagatc
      301 acgcgccgct ttttcgattt ctgttgccgg ctgattacgc ccgaggggtt tcttctgcac
      361 aaatacaacc cggatggatc ggtggggtcg tcatggcatc cgtggctcgc cgacggcaag
      421 cggcaactgc cgattcagga ggacgagacc gcgctggtcg tcgtcgcgct ctggacgcac
      481 ttcgagaagt ttcacgacgt cgagttcgtg aagccgctct acaagccgat catcaagcag
      541 atggccgatt ttctggcgcg ttaccgcgag ccgcacacgc gtctgcccgc gccgtcgtgg
      601 gatttgtggg aggagcggcg cggcatcgcc gccttcacga tcggctcggt ctgggcgggc
      661 ctgtacgccg cagcacgatt cactgacgcc ttcggcgaga ccgcacaggc ggagaagtac
      721 cggcgcgcgg ccgccgagat ccgtgaggcg acgtcgcgct ttctcttcga cgaaacgagc
      781 ggctgctttg cgcgatccgt gaacgtgtcg cgcgaagggg agatcacgac cgacccgaca
      841 ctcgacgcat cgatggccgg actctggctc ttcggaatgt tcgacgccgg cgatccgaag
      901 atcgtggcca cgatggaagc gatgatcgcg cggctaacga tcaagacgga catcggcggc
      961 gtggcgcgct acgagaacga ctactacttt caggtatcga aggatgtgca gaacgttgcc
     1021 ggcaacccgt ggattgtctg ctcgctttcg gtcgcgcact ggtacgcgat gcaggcgcaa
     1081 acgacggctg agctgcagaa agcgatcgac attctgcggt gggcctgcga tcgcgcgctg
     1141 ccgagcggcg tgctcgccga gcagctcgat ccttacaccg gcgcaccgct ctccgttgcg
     1201 ccgctgacct ggagtcacgc ggcgttcgtg ctggccacgc atgtcgtggc acgccgcgcc
     1261 gtcgagctgg cggaaaaagg ctgacgatgg ttcggcgcac ccggcttcgc tcgcgcgtgt
     1321 ggcttctgac ggcggcgttt gcgctcgtgc tcttcgggat cacattcggc ctgagctggc
     1381 gcgcgcaagt ggcgcaggac cgctggagcc ggctcgtcgg tgtcgagacg cgagcgatcg
     1441 caacgctcga agagttgatc cgcgcgcaga acgcgtaccg cgcgcatgcc gtcgctggcg
     1501 acgcgtcccg ctaccggatc gtcgcgcaac tgctcgaatc ggattcgttg tcgacgatcg
     1561 acacggcggc gttgcggcgg aggatggaga cgttccggag cgcgatcgcc gacaacacgt
     1621 cccagcccga cgagatcgat accgagtctc tgcgcgtggt ggccgaggcg cagaggatga
     1681 tcgacgagcg aaagagggag atcgcgcggc aactgccgat gctcgagcgc gaatcgcgcg
     1741 agctgatgac cgcgggacta gccgtggcct ggatcgtcgt catctgcagc ttcgccgcgg
     1801 tgcaggtgac gctacgcaag gtcgtgcgtc cgctcgaaga cctctcccgc gctgccgacc
     1861 gcatcgccgc cggcgacctc ggcgcacgcg cgcaggttgc gggcgatttc gagatcgcca
     1921 atctggctgc ggcggtgaat cacatggcgg atgagctgaa ggcgcatgcc cgcaccgacg
     1981 agctgacgaa cctgccgaac ttccgcgcgt ttcgcgagcg gatcgacctc gagatcgagc
     2041 gcgcaagccg ttatcccgag cacgtcggca tcctcatcct cgatctcgac cgcttcaaga
     2101 aatacaacga cacctacggc cacctcgccg gtaacgatgc gttgcaacgc gtggcaagag
     2161 tgattcgcga gaccgtgcgt tccgtcgatt ttcccgcgcg ctacggcggc gaggagtttg
     2221 ccgtgatcgt cccgcagatc gacgccgcat cgcttgccgc aatcgccgaa cgagttcggg
     2281 aggggatcga gtcgatgtcc gcgcccaagg acggcgccgc ggtgaccgtc agcatcgggg
     2341 cgtcgatctt tcctgacgat ggtgccgccg tcgacgttct cttccgcgtt gccgatgagc
     2401 gtttgtacgc ggcgaagaag gcggggcgaa atcgagtggt cgtcggatcg aaagaactgc
     2461 ggctatcgtc gtgaaagtct tttgaaccac agagacacag agatcacaga ggttttctct
     2521 gtgtcctctg tggctctgtg gttaagcctt tttgagaacg cgttgacacc aacacacccc
     2581 acgcgccacg atcctgcatg accatcgtcc cagcccgaga cgaatcggtg atcgagttca
     2641 ccgcgcgtgc tgtcatcgcc ggcatcttct tcggcattct cttcggcgcc gcgaacgcct
     2701 acctcggact gaaagtcgga ctaaccgtct cgacttcgat cccgatcgcg gtgctgaccg
     2761 tcgcgctctt caagctcatg ccgggccgcc gcgagggcgt gatcctcgaa gcaaatctct
     2821 cgcagaccat cggctcggcc agttcctcgc tggcgaccgg aacgatcttc acgattcccg
     2881 cgctcttttt atgggggata gctccgccgt tctggcaggt atcgatcctc gccctcctcg
     2941 gcgggttcct cggcatcgcg gcgatggtcc cgctgcggcg tttgctgatc gtcaagtcgg
     3001 ccgccgagct gccgtatccg gaaggaactg cgtgcgccga ggtgctaagg gcgacgtcgg
     3061 tgggaaggtc gggcgggaag tggatcttca tcggcctcgc ggttggcggc gcgatcaaac
     3121 tcgccctcgg cgcattcatg ctcattccct cggggttggc gatgcactta ccagtactgg
     3181 ccaaagcgga gctcgcactc gagatcgcgc ctgcgctcct cgctgtcggc tacatcctcg
     3241 gatataagcc ttctgcgatc atggtgtccg gaagcctgat ctctgcgatc gtgctgacac
     3301 cgctcatcgc cacgttcggt gccggtctga cggctccgat gtttccggaa atgaagttgt
     3361 cgatcgcggc gatgacggcg gcgcagatct ggtcgcgcta cgtgcgctac atcggcgcgg
     3421 gcgcggtggc agcggcagga atcgttgcgg tcatcaaagc gctgccgacg atggccagtt
     3481 catttgcggc ggtgatgcgc ggactgcgcg gcggcggact gcagacgatc gatgacgagc
     3541 gcacgccgcg cacggaccgc gatgtgccgt catggatcgt ggtcgtcgtg ccgctggctg
     3601 tgatcattac gctcgtcgcc gtgccgggac ttctcgcggg aaacatgtcc ttcgttccgc
     3661 gtctcgtcgc ggcgatcggg gtggcgatct tcggcgtctg cttcgtcgtc gtgtcgtcgc
     3721 gcatcgtcgg actgatcggc gtctcatcaa acccaacctc ggcgatgacg ctcgtcacgc
     3781 tgctcggcgc atccgtcgtc ttcgtgctgt gcggctggac cgatccctcc gcgcgcgcgg
     3841 cgattctcac cgtcggaacg gtcgtctgta tcgcagcatc gaaagccggc gacatctcgc
     3901 aggatctgaa gaccggattc atcgtcggcg cgacgcccgc caaacagcag ttcggccagt
     3961 tcctcggcgc cgcctgcgcc tgctgggcca tcgctggaac ggtactgctc ctcggcaaag
     4021 ccttcacgtt cggctcgccc gatctccccg cgccccaggc aaccttgatg aagaccgtca
     4081 tcgaaggggt gctctccggc tcgctgcctt gggcgctcgt cggcaccggg tcggcgctct
     4141 cgatctgcgc gatcatcgct ggtctccccg gcctctcctt cgccgtcgga atctatctcc
     4201 ccctcggcac gctggctccg gtcttcgtcg gcggcatcgt ccggcgcatg gtcgaggcga
     4261 agcgcgatgc gaaatcgctc gacagcgatc cgggagtcct ggccgcctcg ggcatgatcg
     4321 ccggcgaagg gctggtcggc gtgctcatcg ccttcctcgt cgctgcgtcc gggaagtttc
     4381 cgggactcgc gagcgcgctg gcgagcatgc acttcgcttc gaaagacttc acctggctca
     4441 aaggctcgat tgcgatcatc accggcatcg cgatcgtgat cgcgatctgc tcgttgctct
     4501 atcgggcagg gcgctcggca ccggccgacg ggagtacacc gaacgacgct ggaatcgtac
     4561 gtagttagtt gattactcga agcacatggc gaagaagaag cgaaaactca cgcgtgcgga
     4621 aaaggctgcg aaggcggttc gcaaggccac cttcgagact atctttatca acggacagca
     4681 gaagagagta cggcggcgca atctgattga aggaatgacc gtggaagact tcattcgtgc
     4741 gaacgctgat ccagtctggc tgcatcagaa cgagatgtgg gagtacatgg ctcccgaacc
     4801 gccggacgat cgcgaggaat caattccaga ccaccagggc gatgagccgc cattttgaag
     4861 ctcccccgag aacctccatg aaacgcacgt ggacagtcat cggcgtaagc gacgtgcctg
     4921 ctagcttcaa gtggtaccag tcgctcttcg gccagcccga gacacaaccg gcccatccct
     4981 actggggtca gatcctcgat tcggatggaa ccgtcctgct ctgcctccac cagtggggcg
     5041 cgcacgagca tccgtcgttg atgagccccg acaacgccac acccggcaac ggactcctcc
     5101 tgttcttccg tgtcgacgat ttcgataggg cgttgatcag agcccgcggc ctcgtcgccc
     5161 ggctcgaaga ggagccccac atgaacccga acaccgaaac tgcggagttc tcactccggg
     5221 atccggacgg gtattacgtc acaatcagtg cgctctctgc ggcctgacag ccgatttcct
     5281 gggagagcag acggaggact gcggaatgct gtcattcact acaagtgagg cgaatgcccc
     5341 gtgtacccca cggtgtcccg caccgtgtat cccacggtcc cgcaccgtgt accccacggt
     5401 cacacaccgc gtatcccacg gtcacacacc g
//
LOCUS       DATLVW010000875         2060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_206621, whole genome shotgun sequence.
ACCESSION   DATLVW010000875 DATLVW010000000
VERSION     DATLVW010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206621"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VNN25_25785"
     CDS             <1..1192
                     /locus_tag="VNN25_25785"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH95011.1"
                     /translation="MFARDPLPGKSSNLFYVRMDNWDPHTPYPSGQNSTGPTIPPQIS
                     YRDAMALMRSNIPMRQSAAFPAMMTVYPDPRVARPSTEGVRLCTSDFFTMFDVPFAYG
                     GPWDKRADAKVEQVVVIDDAMNQKLFGGVNSVGKRVRIEDREFTVAGVLKPWRPFIRM
                     YDLNGNFIAQPEPVYLPFSLTPSMQIRTNGNSDGWKSAGTTFEDFLNSDVDWVQLWVE
                     LRSPAEAEQYHRFVDGYVLEEKKHGRFERPLMNRVTPLRDLMGEFGVITPQVNAMAAI
                     SILFLAICALNLTGLLLGKFLARAPEVSVRRALGATRINVFLQHITECELVGLAGGIL
                     GMLLSIGAMKLIGKFISATSVIALDSEMILAALFLSLGAGLLAGLYPAWRICSVQPAV
                     QLKI"
     gene            1204..>2060
                     /locus_tag="VNN25_25790"
     CDS             1204..>2060
                     /locus_tag="VNN25_25790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH95012.1"
                     /translation="MEFGPIIRALKRNKVRFLLIVVQIAITLAVVTNAITMIRDESVK
                     MTKSSGFDDDNIVWVRTKPFAPGFKDLPYRIAVVNADLRAIKATPGVVAVANTNFLPW
                     QGGGSSGEVAAGDGDGSKFRTQMYYNTPGILDALGVHLVSGRILRDTDIDDDPNSKAP
                     STAIISRDLERLVFKGKSAVGRQLKDSDNTVVNVVGTFDPFYNPYGWPIHEYAIFYAG
                     HVSRRGAMYLVRVAPGSMKQTVPLIEQRMLQTNNGRNIDMKTISEIKDNFFVQGRIVR
                     TAMSLVIIL"
BASE COUNT          405 a          681 c          602 g          372 t
ORIGIN      
        1 catgttcgcg cgcgatccgc tgcccggaaa gagcagcaac ctcttttatg tgcgcatgga
       61 caactgggat ccgcacacgc cgtatccctc gggtcaaaac agcacaggcc cgaccatccc
      121 gccgcagatc agctaccgcg acgccatggc cctgatgcgc tcgaacatcc cgatgcggca
      181 gagcgcggca ttcccggcaa tgatgacggt ctatcccgat ccgcgcgtcg cgcgtccgtc
      241 gaccgagggg gtgcggctct gcacttccga cttcttcacc atgttcgacg ttcccttcgc
      301 gtacggcggg ccatgggaca agcgcgccga cgcgaaggtc gagcaggtcg tcgtcatcga
      361 tgacgccatg aatcagaagc tcttcggcgg cgtcaactcg gtaggcaagc gcgtgcgcat
      421 cgaagatcgc gaattcaccg tcgccggcgt gctgaaaccg tggcgcccgt tcatcaggat
      481 gtacgacctg aacggaaact tcattgccca accggagccg gtctacctgc cgttctcgct
      541 gacgccgtcg atgcagatcc gaacgaacgg aaacagcgac ggctggaaat cggccggcac
      601 caccttcgag gattttctca actccgacgt cgactgggtg cagctctggg tcgagctgcg
      661 ttcgcctgcc gaggccgagc agtaccaccg cttcgtcgat ggctacgtgc tcgaagaaaa
      721 gaaacacggc cgcttcgagc gcccgctcat gaaccgcgtc acgccgctgc gcgatctgat
      781 gggggagttc ggcgtgatca ctccccaggt caacgccatg gccgccatct ccatcctctt
      841 cctcgccatc tgcgcgttga acctgaccgg gctgctgctc gggaaatttc tcgctcgcgc
      901 acccgaggtg tcggtgaggc gggccctcgg cgcgacgcgc atcaacgtat tcctgcagca
      961 catcaccgag tgtgagctgg tcggcctggc cggcggcatc ctcggcatgc tgctctccat
     1021 cggcgcgatg aagctcatcg gcaagttcat ttcggcgacc tcggtcatcg cactcgacag
     1081 cgagatgatc ctggccgcgc tcttcctgtc cctcggcgcc ggacttctgg ccggacttta
     1141 cccggcgtgg cggatctgtt ccgtgcagcc agccgtgcaa ctcaagatct gaaggagacg
     1201 accatggaat ttggaccgat catccgcgcc ctgaaacgca acaaagtccg ctttctgctc
     1261 atcgtcgtac agatcgccat cacgctcgcc gtcgtgacga acgcgatcac gatgatccgc
     1321 gacgaaagcg tgaagatgac gaagagctcc ggcttcgacg acgacaacat cgtctgggtc
     1381 cgcaccaaac cgttcgctcc cgggttcaag gatctgccgt accgcatcgc tgtagtgaac
     1441 gccgaccttc gcgctatcaa ggccacgccg ggagtcgtcg cggtggccaa cacgaatttc
     1501 ctgccatggc agggcggcgg cagctccggc gaagtcgctg ccggcgacgg cgacggcagc
     1561 aagttccgca cgcagatgta ctacaacacg cccggcatcc tcgacgcgct cggagtgcat
     1621 ctcgtttcgg gccggatctt gcgcgacacc gacatcgacg acgatccgaa ctcgaaagcg
     1681 ccgagcacgg ccatcatctc gcgcgatctc gagcggctcg tgttcaaagg aaagagcgcc
     1741 gtcgggcggc agctcaagga ttccgacaac accgtcgtca acgtcgtcgg tacgttcgat
     1801 ccgttctaca acccgtacgg atggccgatc cacgaatacg cgatcttcta cgccggacat
     1861 gtgtcgcgcc gcggcgccat gtatctcgtc cgcgtggcgc cgggctcgat gaagcagacc
     1921 gtgccgctga tcgaacaacg catgctgcaa acgaacaacg gacgcaacat cgacatgaag
     1981 acgatcagcg agatcaagga caacttcttc gtgcagggaa ggattgtgcg caccgcgatg
     2041 tcgctggtca tcattctgat
//
LOCUS       DATLVW010000876         2101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_371096, whole genome shotgun sequence.
ACCESSION   DATLVW010000876 DATLVW010000000
VERSION     DATLVW010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371096"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            621..1457
                     /locus_tag="VNN25_25795"
     CDS             621..1457
                     /locus_tag="VNN25_25795"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXH95013.1"
                     /translation="MTTDLDRRASPIRRKAPPQIPDWLRFVLEVPLEMKLLGANLIIV
                     AVAGLLLFGPVQLQPERLTDAYIVVVALIFGATVNLVLVRLALGPIESLERVSRWVSE
                     GRIAERVPTSIVADHELARLSKTINEMLDSLAAGRERVERLGAEVIYAEERNRSQVAQ
                     ELHHSVGQKLADASFHIAAAANETDAYARSSRLAEAKDQLRRAIDEIRTISGSSHPHV
                     AADLGLPGMLEHLGNVTRQRSVTNVRPRVDDHQGLIPRGDGVVVAHDIGDMGPRSPET
                     RQ"
     gene            1454..1828
                     /locus_tag="VNN25_25800"
     CDS             1454..1828
                     /locus_tag="VNN25_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXH95014.1"
                     /translation="MITAKRAYEPADPSDGYRVLIDRLWPRGVSKAKARLDAWEKEIA
                     PSRELREWYDHDPAKWPEFRARYKKELRVATAKMILADLVRRARRGRVTLVYGSRAAE
                     ISDVAVLEGMLRRRVRTADQKR"
     gene            1854..2072
                     /locus_tag="VNN25_25805"
     CDS             1854..2072
                     /locus_tag="VNN25_25805"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXH95015.1"
                     /translation="MLRPGNLYAFDDTCTHAGCSLARGKLDGTTVTCPCHGSQFDVTS
                     GVVLRGPAKRPVRSRLVKVEGENLLAEA"
BASE COUNT          444 a          603 c          637 g          417 t
ORIGIN      
        1 gagacactgc ccattccatc agtgcgggcg cagtacaggt gcggcagcac cacgcgaatg
       61 gtgctgtggg ctgcgcccgg accaatattg gtgctgccac tcgaatcggg tgcggctcaa
      121 gccctaccgc ttgcgatgaa gacgcgacag tcggatatgc acgtaccgac acgcgctgaa
      181 ccgctcgcac tgggattata acaatgctgc ctgacagcga atcatcggga acgaggaccc
      241 cacgtccgga tctggacgag gttaatgacg cttcgttcga cagctttcca gccagcgacg
      301 cgcctacctg gagctcgatg cacgctggcg cgccgcagtt aagcgaatct tcttctgcgt
      361 ccaaatgcat ctctggcaat cctggcaagg accgcgacct ggggatttag tgccaactac
      421 tatgagccga tgcctctgcg cgtgtgactc cctcaagtcg cttattggtc ggacaaagtg
      481 ttggtgacta aacagagatt cttctcttct tcacgctttc ttcacgagag atcagctagt
      541 ctccggcgca agtagaccag accccttcac tcgcgcattc tcgctgagca ctgggtgtcc
      601 gccacaggag cccgaaagta atgacaaccg atctagatcg tcgagcttcg ccgatccgcc
      661 gaaaggcacc gcctcagatt ccggactggc tgcgtttcgt cctcgaggtt ccgctggaga
      721 tgaagctgct cggcgcgaat ctgatcattg tggcggtggc gggtctactt ctgttcggac
      781 cggtccagtt acagcccgag cggctgaccg acgcctacat cgtcgtggtt gctctgattt
      841 tcggggcgac agtgaatctc gtgcttgtga gactcgcgct cggtccgatc gagtcgctcg
      901 agcgtgtctc gcgatgggtt tctgagggtc gaatcgccga gagagttcct acgtcaatcg
      961 tagccgacca cgagctggca cggctctcga aaacaattaa cgagatgctt gacagtctcg
     1021 cggccggccg cgagcgagtg gaaaggctcg gcgcagaagt gatatacgct gaggaaagga
     1081 atcgctcgca agttgcccag gaacttcacc attccgtggg ccaaaagctc gcggacgcga
     1141 gttttcatat tgccgcggct gccaacgaga cggacgctta cgcccgttcg tctcgactcg
     1201 cggaggcaaa agatcaattg cgcagggcaa tcgacgaaat ccgcaccatc tctggctcgt
     1261 cacatcctca tgtggccgca gatctcggtt tgcccggaat gctcgaacac ctcggtaacg
     1321 tcactcggca gcgttccgtt acaaatgtcc ggccaagggt agatgaccac caagggctga
     1381 tcccgcgtgg agacggtgtc gtagtggctc acgatatagg agacatggga ccccgaagcc
     1441 cggagacgag acaatgatta ccgcgaagcg agcgtatgaa ccggcagacc cgtccgatgg
     1501 ataccgggtc ttgattgatc gcctttggcc acgcggcgta tcgaaagcga aagcgagact
     1561 ggatgcatgg gagaaagaga tcgccccgtc ccgggagctc cgcgagtggt acgaccacga
     1621 tcctgccaag tggcctgaat tccgcgcgcg gtacaaaaag gaacttcggg ttgcgacggc
     1681 aaagatgatt ctcgccgatc tggtgcggcg cgcccgacgt ggacgggtga cgctcgttta
     1741 tggctcgcgc gccgctgaga tcagcgacgt ggccgtgcta gaggggatgt tgagacgccg
     1801 cgttcgcacg gcagaccaaa aacgttgact ccgagactga ccagaatggt caaatgttga
     1861 ggcctgggaa tctgtacgcc tttgatgaca cgtgtactca cgcaggatgc tcgctcgctc
     1921 ggggcaagct ggatgggacg acggtgacct gtccctgcca cggcagccag ttcgacgtca
     1981 cttcgggcgt cgttctcagg ggaccagcca aacgcccagt gcgttcccgg ttggtgaaag
     2041 tcgagggcga gaatttgctc gccgaggctt gaagaaattc gcgaatccag aactgaggac
     2101 t
//
LOCUS       DATLVW010000877         9408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_120051, whole genome shotgun sequence.
ACCESSION   DATLVW010000877 DATLVW010000000
VERSION     DATLVW010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120051"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..528)
                     /locus_tag="VNN25_25810"
     CDS             complement(13..528)
                     /locus_tag="VNN25_25810"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HXH95016.1"
                     /translation="MKRLALLLLPVLATSCGYALVGNANNLLGPTVHTIHVPAFVNKT
                     TRVELEQRVTQAVAAEFVSRGRLKLVNTPPEADVILRGSIDSFGIFPVAFNAQGRATQ
                     YQISITAKIELLDHKADDKVLWKNDQYRFAENYQVNTESTDAFDQETRAINEIAVRFA
                     ESLVTNLLEGF"
     gene            complement(525..1016)
                     /locus_tag="VNN25_25815"
     CDS             complement(525..1016)
                     /locus_tag="VNN25_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95017.1"
                     /translation="MRKNFLSVGEIFLSAGKIFLSVGKIFLSVGEIFLSIGEIFLSVG
                     KIFLSVGKNSLYGGEKLLQVERSFLSEEQILSQPPRVSRTPAEIFQNREEIIETPAEN
                     TPPERCRLVLETEQTSWVTFKMGSGLILKVTSLRAERGTLVCHFQNQTCPHFESDVLP
                     FPA"
     gene            complement(1185..3743)
                     /gene="leuS"
                     /locus_tag="VNN25_25820"
     CDS             complement(1185..3743)
                     /gene="leuS"
                     /locus_tag="VNN25_25820"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXH95018.1"
                     /translation="MPYEPKLIEEKWQSAWSDARVAEVDVNAPGEKFYMLNMFPYPSG
                     DLHVGHGRNYILGDALYRFFRMQGRAALNPMGWDAFGLPAENAAIKRGIHPRQWTVSN
                     IEREKKQFLRWGILYDWSKELASCEPEYYRWNQWLFLQLFKRGLAYRAKAPVNWCPSD
                     QTVLANEQVIDGRCERCNSIVIQRDLEQWFLKITDYADRLDAGLDTLEHWPEKVKLMQ
                     RNWIGRSVGADVDFAVPSLGKVMRIFTTRPDTIFGATFMVLAPEHPDVATLIADHSDR
                     AGIEEWIKGVRNQSNLERQEAGKEGRFTGKTATNPFTNEQIPIWLGNFVLMQYGTGAI
                     MAVPGHDQRDFEFARQYNLPVRIVVRPSAGAVPDADTMPEAFVVKDESAMAVNSGLID
                     GLPTPKAIATIIDEIERLGIGKKMVRYRLRDWLISRQRYWGTPIPMIYCERDGIVPVP
                     EEQLPVELPLDVPFTGREGNPLAKDERFVNTTCPKCGGAAKRETDTMDTFVDSSWYYA
                     RFISANDDTKIVDTPLANRWLPVDQYIGGIEHAILHLLYARFICRTLHDMGLLAYEEP
                     FTRLFNQGMITKEGYRDAEHGTWVAIADVEWIDGKPVQKATGRALIAEISKMSKSHFN
                     VVPPDELIDKYGADTERVYTLFIAPPEKEAAWSDEGVVGAHRFLGRVWHMGEQILGEN
                     HQSDGSRGQALIRKTHQTIDTITRRFDRFEFNTAISGLMELSNAIGDAITSGDADAAT
                     LRQSYTTLLKLLHPFAPHITEELWLMFGNKPFILTSDWPKADPALMVEDVVTIVVQVN
                     GKLRGQVEVPNPPEKEVVLAAIRANKKVQQWLAGKEVVKEVYVPGKLVNIVIKG"
     gene            complement(3911..4348)
                     /gene="dtd"
                     /locus_tag="VNN25_25825"
     CDS             complement(3911..4348)
                     /gene="dtd"
                     /locus_tag="VNN25_25825"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXH95019.1"
                     /translation="MKVVIQRVRRASVSVGDELVSEIGPGLLLLAAVEKGDAEQVMRD
                     AAKKIRELRIFSDDAGKMNRDVTEAGGSILAVSQFTLAGSIARGRRPSFDNAEEPERA
                     KALFELFVSELASTGISVKTGRFREMMMVSLENDGPVTFVWEG"
     gene            4517..4945
                     /locus_tag="VNN25_25830"
     CDS             4517..4945
                     /locus_tag="VNN25_25830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXH95020.1"
                     /translation="MINKVILVGRLGKDPEMRSTPGGQTVAKFTIATDERYTDKNGEK
                     QERTEWHNIVAWGKLAEICGQYLRKGKLVYIEGSLRTDSWEDKETKQKKYRTEINANT
                     MKMLDRKGDDEGGGGYTGARKQAPVSSGAPQMEDDDEVPF"
     gene            5051..5674
                     /locus_tag="VNN25_25835"
     CDS             5051..5674
                     /locus_tag="VNN25_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXH95021.1"
                     /translation="MYAGERFNSITHIAGSVLAIVGTVVAVGSAVSRGRDGETITAIA
                     IYGAMMIVLYLSSTLYHSLRGRAKNVFHVFDHCAIYLLIAGTYTPITLITLRGRWGWW
                     LFGIVWTLAIAGVLKDTLLRGRYRPVSVVLYVLMGWLVVVAIQPLRMAMPSRGIVWLA
                     AGGLAYTIGIVFFALSKHVVHAHGVWHLCVIGGSACHYVAVTRYVLL"
     gene            complement(5652..6197)
                     /locus_tag="VNN25_25840"
     CDS             complement(5652..6197)
                     /locus_tag="VNN25_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH95022.1"
                     /translation="MTNPGRPEAGEYNTYYQRYIDLATEDDVVGALDAQAHETATLLG
                     GLTEEQAAHRYAPDKWSVKQVVGHIIDAERIFAYRALSIARGETKPLPGFDQEPFVIN
                     ARSDDRSLSDLAEELAAVRRGNVMMMRALSDDAWRRVGTASENPVSPRAIAYIMLGHE
                     RHHLRIVRERYLGAPTAARTA"
     gene            6228..6740
                     /locus_tag="VNN25_25845"
     CDS             6228..6740
                     /locus_tag="VNN25_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH95023.1"
                     /translation="MEEVTISRLSGDAEAQVCAQMMASSDPWLHFGRTFEQCLARVTN
                     PSGEVWVARTGGVPRGFIILVLQGAFVGYIQIVCVAESARGSGLGSRLVAFAEERIFR
                     EFPNVFLCVSSYNPRARALYERLGYKLVGELHDYILPGESEFLMRKSVGPLVEFKPFD
                     HGDTEVTEKT"
     gene            complement(6771..9323)
                     /locus_tag="VNN25_25850"
     CDS             complement(6771..9323)
                     /locus_tag="VNN25_25850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH95024.1"
                     /translation="MKIRAQLVLACFLLSVLPLSVIVIYSYESSRRALEAAYRREADA
                     RTKQMDRRLATIRGDLQQRLAEVSALPMTNGASDRTPDVGNILLTMGDAASLVDSLEI
                     RPVRFDSLPNVQAKVQTIVREQIRHVAGANNAPKVKRNAPPDVEEPDAPPEAPAVTAA
                     EVAADAADEVDAMPSPGATPGGAAPTMPPGAPRPPMLPRFTMSPEQRAAINEIATLSA
                     KLGESFQTMPPGDRTVLEARIQVLQRSLNDSLRATQTQWKQQFDDAQRRREMYFAARQ
                     QQRVAAHDELRRAHDAGREAREHAFPAAPGVAPTPAPAAAPQIAAVHPTPAPNAAEDV
                     VVNSKPADAVRVHEHPASSLFGQKFNFPLRKEGTIVGHISAQVSTPEVVRRVLGSRSD
                     DNDEITFALDNANNVYTRTPAERKTLDSAGITDRVLKNRPLNDVHDWIVQMSRDPQSG
                     LRVGVARPVGDNLEELRKTAGKNFGYGIALIFVAMIGIVPIANHMTRDVNLVTRGAER
                     IAQGDLMTRLPVKSKNEIGQLAAAFNRMAQDLSLQQQTIVEQERTRKEQEIQQRLLEL
                     EYERKSIDLEDARRFQLSMLPKSVPMHDRYDVAVFTLTAAEVGGDYYDFHVAPGNVGR
                     TNVGRTLQSDAVDGSLREDNTSSRTGESDLRKPSQDSVMTVTIGDATGHGAKAGTMVT
                     VVKALFSGYVPEVTPAEFLRDAAEKVKRMDLGRMAMALLLARFDRERLTVASAGMLPA
                     YVHRAQGGKIEEVARGATPLGTLGADYHDVVIDLAAGDTVLFMTDGFPELQNESGQQL
                     GYGAAVEHFATAASGATADDVIASLASAAKQWNGERPPNDDVTFVVVRAKTG"
BASE COUNT         1703 a         2869 c         2973 g         1863 t
ORIGIN      
        1 cgaagcgcag tactaaaacc cttccagcag gttcgtcacc agcgattccg cgaaccgcac
       61 ggcgatctcg ttgatggcgc gggtctcctg gtcgaaggca tcggtggact ctgtgttgac
      121 ctggtagttc tcggcgaagc ggtactggtc gttcttccag agcactttgt cgtcggcctt
      181 gtgatcgagc agctcgatct tggcggtgat ggagatctga tattgggtcg cccgcccctg
      241 ggcgttgaag gcgacgggga agatcccgaa ggagtcgatc gatccgcgca ggatcacgtc
      301 ggcctcgggg ggcgtgttca caagcttcag ccgcccgcgc gaaacgaact ccgcggccac
      361 cgcctgcgtg acgcgctgct cgagctcgac gcgcgtggtc ttgttcacga atgccggcac
      421 gtggatcgta tgcaccgtag gcccgaggag gttgttggca ttgccgacca gcgcgtagcc
      481 gcacgatgtg gccagaaccg gcaggagcag aagtgcgagg cgtttcatgc cgggaaaggt
      541 aacacgtcac tttcaaaatg ggggcaggtc tgattttgaa agtgacaaac gagcgtccct
      601 ctctcagcgc gcaggcttgt cactttcaaa atcagacctg accccatttt gaaagtgacc
      661 cacgacgtct gctccgtttc cagcaccagt ctgcatcgct cgggcggtgt gttttcagcc
      721 ggcgtctcga tgatttcttc ccgattctga aagatctcag ccggcgtccg ggagactcgg
      781 ggcggttgcg aaagaatctg ctcctcggag agaaagcttc gttccacctg gagaagtttc
      841 tctcccccgt aaagagagtt ctttcccacc gaaagaaaaa tctttccaac cgaaagaaaa
      901 atctctccaa tcgaaagaaa aatctctcca accgaaagaa aaatctttcc aaccgaaaga
      961 aaaatctttc cagccgaaag aaaaatctct ccaaccgaaa gaaagttctt tcgcacctaa
     1021 agaaagttct ctccaaccca gagaaagttc tctccgacct agagaaagtt ctctccgacc
     1081 tagagaacgt ttcactccgg ctagagagag ttccgctcta cctggagaaa gttctgctcc
     1141 agatggagaa cgtttcactc cacatggaga gagtttcgcg cccgctaccc tttgatcacg
     1201 atattgacca gtttccccgg cacgtacacc tctttcacta cctctttgcc ggccagccac
     1261 tgctggacct tcttgttggc gcggatcgcg gccaggacga cttccttctc cggcggattc
     1321 ggcacttcga cctgaccgcg cagcttgccg ttgacctgca cgacgatcgt cacgacgtct
     1381 tccaccatca gcgccggatc cgctttcggc cagtccgagg tgaggatgaa gggtttgttg
     1441 ccaaacatca gccagagctc ttccgtgatg tgcggcgcga acggatggag gagcttgagc
     1501 agcgtcgtgt acgactggcg cagcgtcgcc gcatcggcgt cgcctgacgt gatcgcgtcg
     1561 ccgatggcgt tcgaaagctc catcagcccg gagatggcgg tgttgaactc gaaacggtcg
     1621 aagcggcgcg tgatggtgtc gatcgtttga tgcgttttgc ggatcagagc ctggccacga
     1681 ctgccgtcgg attgatgatt ttctccgagg atctgctcgc ccatgtgcca gacgcgtccc
     1741 aggaagcggt gcgcgccgac gacgccttcg tccgaccatg ccgcttcctt ctccggcggc
     1801 gcgatgaaca acgtgtagac gcgctcggtg tccgcgccat atttgtcgat cagctcgtcg
     1861 ggcgggacca cgttgaagtg cgacttcgac atcttgctga tctcggcgat cagcgcgcgg
     1921 cccgtcgctt tctgaacggg ctttccgtcg atccactcca cgtcggcgat ggccacccag
     1981 gtaccgtgct cggcgtctcg gtagccctcc ttcgtgatca ttccctgatt gaataagcgc
     2041 gtgaacggct cttcgtacgc cagcagcccc atatcgtgca gcgtgcggca gatgaagcgc
     2101 gcgtagagga gatggaggat ggcgtgctcg atgccgccga tgtattgatc gaccggcagc
     2161 cagcggttgg ccagaggcgt gtcgacgatc ttcgtgtcgt cgtttgcgga gatgaaccgc
     2221 gcgtagtacc acgacgagtc gacgaacgtg tccatcgtgt cggtctcacg tttcgccgcg
     2281 ccgccgcact tcggacatgt ggtgttgacg aagcgttcgt ccttcgccag cggatttccc
     2341 tcgcgtcccg tgaacggcac atccagtggc aactccaccg gcaactgctc ctcggggacc
     2401 gggacgatcc cgtcgcgctc gcagtagatc atcgggatcg gcgtgcccca gtagcgctgc
     2461 cgcgagatga gccagtcgcg cagccggtag cgaaccatct tcttgccgat gccgaggcgc
     2521 tcgatctcgt cgatgatcgt agcgatggct ttgggcgtcg ggagtccgtc gatgagacct
     2581 gagttgaccg ccatcgcgga ttcgtctttg acgacgaacg cttccggcat cgtgtccgcg
     2641 tccggtacag cgcccgccga tggacggacg acgatgcgga cgggcaggtt gtactggcgt
     2701 gcaaactcga agtcgcgctg gtcgtgtccc ggcacggcca tgatcgcgcc ggtgccgtac
     2761 tgcatcagca cgaagttacc gagccagatc gggatctgct cgttcgtgaa cggattggtg
     2821 gcggtcttgc cggtgaagcg accttccttc cccgcctctt gccgctcgag gttggattgg
     2881 ttccgcacgc ctttgatcca ctcttcgatc ccggcgcgat cagagtgatc cgcgatcaac
     2941 gtcgccacat cgggatgctc cggcgcaagc accatgaacg tcgcgccgaa gatcgtgtcc
     3001 gggcgcgtcg tgaagatgcg catcactttg ccgagggagg gaacggcgaa gtcgacgtcc
     3061 gcgccgacgg accgtccgat ccagttgcgc tgcatcagct tcactttttc cggccagtgc
     3121 tcgagcgtat cgagaccggc atcgaggcgg tcggcatagt cggtaatctt gaggaaccat
     3181 tgctcgaggt cgcgctggat gacgatcgag ttgcagcgct cgcagcggcc gtcgatcacc
     3241 tgctcgttag cgagcacggt ctgatcggac ggacaccagt tcaccggggc tttcgcgcgg
     3301 tacgcgagac cgcgcttgaa gagctgcagg aagagccact ggttccagcg gtagtactcg
     3361 ggctcgcacg aggccagctc tttcgaccag tcgtagagga tgccccagcg cagaaactgc
     3421 ttcttctcgc gctcgatgtt cgagacggtc cattgccgcg gatggatgcc tcgcttgatt
     3481 gcggcattct cggcaggcag accaaaggca tcccatccca tcggattgag cgcggcacgg
     3541 ccctgcatgc ggaagaaacg gtagagggcg tcgccgagga tgtagttgcg gccgtgtccg
     3601 acatgaagat cgccggaggg gtacgggaac atgttcagca tgtaaaactt ctcgcccggc
     3661 gcgttcacat caacctcggc cacgcgggcg tcggaccagg cgctctgcca cttctcttcg
     3721 atcagtttgg gttcgtaagg cataggcggc ggagtatagc ggtgggcaca gtcgaagcgg
     3781 ttagagagaa gagatcagag accagagaca agagatcagg gatcagagac caggattaac
     3841 caggaatcag agatcaggtg acctgatctc tgatctcttg tctctcttgt ctcttgtcgc
     3901 ctgatcgttc tcaaccctcc cacacgaacg tgaccggtcc gtcgttctcg agcgagacca
     3961 tcatcatttc gcggaagcgg ccggtcttca ctgagatgcc ggtcgacgca agctcgctga
     4021 cgaatagctc gaacagcgct ttcgcccgct ccggctcctc ggcgttgtcg aaactcggcc
     4081 ggcgtccgcg ggcaatcgat cctgccagtg tgaactgcga gaccgccaga atcgaaccgc
     4141 cggcctcggt gacgtcgcgg ttcatcttgc cggcgtcgtc gctgaagatg cgtagctcgc
     4201 gaatcttctt cgcggcgtcg cgcatgacct gctcggcgtc ccctttttcg acggcggcga
     4261 ggagcaggag gccgggaccg atctcggaga cgagctcgtc accgacgctc acggaggcgc
     4321 gtctcacgcg ctggatcaca accttcacag agacggattc tacccgtggt agaaaacgcg
     4381 attcctgcgc aaccttcgat tacaattcgt ccacccatca cgagaccgcg cctcaagggc
     4441 tgcgccggcc aaggacaggg cgcaagccgc tggcgttcgc cggcggacga acaacaaaca
     4501 cgaaggagtt ttcaacatga tcaacaaagt cattctggtc ggacgcctgg gaaaggatcc
     4561 ggagatgcgg tccacccccg gcggccagac cgtcgcaaag ttcacgatcg ccaccgacga
     4621 gcgctacacc gacaagaacg gtgagaagca ggagcgcacc gagtggcaca acatcgtcgc
     4681 ctggggcaag ctcgccgaga tctgtgggca gtacctccgc aagggaaagc tggtctacat
     4741 cgaaggctcg ctccgcacgg atagctggga agacaaagag acgaagcaga agaagtaccg
     4801 cacggagatc aacgccaaca ccatgaagat gctcgaccga aagggcgacg acgaaggtgg
     4861 cggcggatac acgggtgcgc gcaagcaggc gccagtctcg tccggtgcgc cgcagatgga
     4921 agacgacgac gaagttccgt tctagttttt gttggttcac gcggaggctc cgcgactccg
     4981 cgtctccgcg tgaaccgtct cctgaattgc cccgacgcac acgaaaccat ccccatgcta
     5041 gattcgccgc atgtacgcgg gggaacgttt caacagcatc acgcacatcg ccggctcggt
     5101 gctggcgatc gtcgggacgg tagtcgcggt cgggtccgcg gtctcacgcg gccgtgacgg
     5161 cgagacgatc acggccatcg ccatctacgg cgcgatgatg atcgttctct atctcagctc
     5221 gacgctctac cactcgttgc gcggccgggc caagaacgtg tttcacgtct tcgatcactg
     5281 cgcgatctat ctgctgattg ccgggacgta cacgccgatc acgctgatca cgttacgcgg
     5341 acggtggggc tggtggctgt tcggcatcgt ctggacgctg gccatcgccg gcgtgttgaa
     5401 ggacacgctg ctgcgcggcc gctaccgtcc ggtctcggtc gtgctttatg tgctgatggg
     5461 atggctcgtc gtcgtggcca tccaaccgct gcggatggca atgccttcgc gcggcatcgt
     5521 ctggctcgcc gcgggcggac tggcctacac gatcggtatc gtcttcttcg cactctcgaa
     5581 gcacgttgtt cacgcacacg gcgtctggca cctctgcgtg atcggcggca gcgcctgcca
     5641 ctacgtcgcg gttacgcggt acgtgctgct gtaggggcgc cgaggtaacg ctcgcggacg
     5701 atgcggagat ggtgccgctc gtgcccgagc atgatgtaag cgatcgcgcg cgggctgacg
     5761 ggattctcgc tggccgtgcc gacgcggcgc caggcgtcat ccgacaacgc acgcatcatc
     5821 atcacgttgc ccctccgcac cgccgcgagc tcctcggcga gatcggacag cgatcgatcg
     5881 tcggatctgg cgttgatgac gaacggctcc tgatcgaatc ccggcaacgg tttcgtctcg
     5941 ccacgcgcga tcgaaagcgc gcgatacgcg aagatgcgct cggcgtcgat gatgtgaccg
     6001 acgacttgtt tgacgctcca tttgtcgggc gcgtaacgat gcgcggcctg ctcctcggtg
     6061 agtccgccga gcagcgtggc cgtctcgtgc gcctgcgcgt cgagcgcgcc gacgacgtcg
     6121 tcttcggtag caagatcgat gtagcgctga tagtaggtgt tgtattcgcc ggcctcgggc
     6181 cttcctggat tcgtcatccg cgcatcatcg cgtagactcg ctgcaaaatg gaagaggtga
     6241 caatcagcag gctttccggt gacgccgagg cgcaggtgtg cgcgcagatg atggcgtcga
     6301 gcgatccgtg gctgcacttc gggcgaacgt tcgagcaatg ccttgcgcgc gtaacgaatc
     6361 cgtccggcga ggtgtgggtg gcgcgcaccg gcggtgtgcc gcggggattc atcatcctcg
     6421 tcttgcaagg agcgttcgtc ggttacatcc agatcgtctg cgtcgccgaa agcgcgcgcg
     6481 gcagcggcct cggatcgcgt ctggtcgcgt tcgccgagga gcggatcttc cgcgagttcc
     6541 cgaacgtgtt tctctgcgtg tcgtcgtaca acccgcgagc ccgtgcgttg tacgaacggc
     6601 tcggatacaa actggtcggc gagctgcacg actacatcct gcccggcgag tcggagtttc
     6661 tgatgcggaa gtcggtgggg ccgttggtcg agttcaaacc ttttgaccac ggagacacag
     6721 aggtcacaga gaaaacctag aaccctctgt gacctctgtg tctctgtggt tcaacctgtc
     6781 ttcgcgcgca ctacgacgaa cgtcacgtcg tcattcggtg gccgctcgcc gttccattgc
     6841 ttcgccgccg aggcgagtga tgcgatgacg tcgtccgcgg ttgcgccgga tgcggccgtc
     6901 gcgaagtgct cgactgcggc gccgtagcct agttgctggc cggactcgtt ctgcaactct
     6961 gggaagccgt cggtcatgaa gaggacggtg tcaccggcgg cgaggtcgat gacgacgtcg
     7021 tggtagtcgg cgccgagggt gccgaggggg gtggcgccgc gggcgacttc ttcgatcttt
     7081 ccgccctgcg cccgatgcac gtacgcgggg agcattccgg ccgacgccac cgtaagccgc
     7141 tcgcggtcga agcgagccaa cagcagcgcc atggccatac gcccgaggtc catgcgcttc
     7201 actttctcgg ccgcgtcgcg cagaaactcc gccggcgtca cctcgggcac atagcccgag
     7261 aagagcgcct tgacgacggt gaccatcgtc cccgctttcg cgccgtggcc ggtggcgtcg
     7321 ccgatcgtca cggtcatcac gctgtcttgg cttggcttac gtaggtcgga ctctccagtc
     7381 cgactggacg tgttgtcttc gcgaaggctg ccatcaacgg cgtcggactg gagagtccga
     7441 cctacgtttg tccgacctac attgcctggc gcgacgtgaa agtcgtagta gtcgccgccg
     7501 acttcggccg cagtcagcgt gaaaacggcg acgtcgtagc gatcgtgcat cggaaccgac
     7561 ttcggcagca tcgagagctg gaaacgccgt gcgtcctcga ggtcgatcga tttccgctcg
     7621 tactcgagct ccagcaatcg ctgctgaatc tcctgctcct tgcgcgtccg ctcctgctcg
     7681 acgatggtct gctgctgcag gctgagatcc tgggccattc gattgaaggc ggcggcgagc
     7741 tggccgatct cgttcttgga cttcaccgga aggcgcgtca tcaggtcgcc ctgcgcgatg
     7801 cgttcggcgc cgcgcgtgac gaggttcacg tcgcgtgtca tgtggttggc gatcgggacg
     7861 atgccgatca tcgccacgaa gatgagcgcg atgccgtagc cgaagttctt gccggcggtc
     7921 ttgcgcaatt cctcgaggtt gtcgccgacc ggccgcgcaa cgccgacgcg caggccgctc
     7981 tgcggatcgc gcgacatctg cacgatccag tcgtgcacgt cgttcagcgg gcgattcttc
     8041 aggacgcggt cagtgattcc ggcgctgtcg agagtcttcc gctcggcggg cgtgcgcgtg
     8101 tagacgttgt tggcgttgtc gagtgcaaac gtgatctcgt cgttgtcgtc gctgcgcgat
     8161 ccgaggacgc ggcgaacgac ctcgggcgtg cttacctgcg ccgatatgtg gccgacgatc
     8221 gttccttctt tgcgcagcgg gaagttgaac ttctggccga agagagacga cgccgggtgt
     8281 tcgtgtacgc gaacggcatc tgccggcttg ctgttcacga cgacgtcttc tgcggcattg
     8341 ggagctggtg tcggatggac ggcagcaatc tgcggcgcgg cggcgggtgc aggagtggga
     8401 gcaaccccag gagcggcggg gaacgcgtgc tcgcgcgctt cacgcccggc atcgtgcgca
     8461 cgccgcaact cgtcgtgagc ggcgacacgc tgctgctgcc gggcggcgaa atacatctcg
     8521 cgccgcctct gcgcgtcatc gaactgctgc ttccactgcg tctgcgtggc gcgcagcgag
     8581 tcattgaggc tcctctgcag cacctggatt ctcgcttcga ggaccgttcg atccccaggc
     8641 ggcatggtct gaaagctctc gccgagcttc gcactgagtg tagcgatctc gttgatcgcc
     8701 gctcgctgct cgggcgacat cgtgaaacgc ggcagcatcg gagggcgcgg cgcgccggga
     8761 ggcatcgtgg gcgcggcacc gcccggagtt gcacccgggc tcggcatcgc atcaacttcg
     8821 tcggccgcgt ccgccgctac ctcggccgca gtgactgcag gagcttccgg aggcgcatcc
     8881 ggctcttcaa cgtccggtgg agcgttgcgc ttcactttcg gcgcattgtt cgcgccggcc
     8941 acgtgccgta tctgctccct cacgatcgtt tgaactttcg cctgtacgtt cggaagcgaa
     9001 tcgaaacgaa cgggacgaat ctcgagcgag tcgacgagcg atgccgcgtc gcccatcgtc
     9061 agcaggatgt tgccgacatc gggcgtacgg tccgacgctc cgttcgtcat cgggagcgcc
     9121 gatacctcgg ccagccgttg ctgcagatca ccgcggatcg tggccaggcg ccggtccatc
     9181 tgtttcgtac gcgcgtcggc ctcacgccga tatgcagcct cgagggcgcg gcgcgaggac
     9241 tcatacgagt aaatgacgat gacactcaac ggaagaaccg agaggaggaa acaggcgaga
     9301 acgagctggg cgcggatttt catcgggcag ttgcgatggt aagacgaaaa tggccggaga
     9361 aggtttggaa cgaggcaagg gacaagtgac aatggagagt tgacaatg
//
LOCUS       DATLVW010000878         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_120217, whole genome shotgun sequence.
ACCESSION   DATLVW010000878 DATLVW010000000
VERSION     DATLVW010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120217"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VNN25_25855"
     CDS             <1..1128
                     /locus_tag="VNN25_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXH95025.1"
                     /translation="PDASKGTWPAAQVPSGNPLEALLLDAARRGASDLHIIAGSVPVF
                     RVGGRLVRGEGSVLSDDDVAPLLDPLLTPRVRERIAEDGAIDFSLRLDAPAVSEERRA
                     WRFRVNIHRQRGTFAASIRSLPNAVPTIAELNLPPILDELVKPTRGLVLICGPTGAGK
                     TTTLAAMIGAINRAESRHIITIEDPIEYEHRNAQSVIEQIEVGRDAPSFASALRAALR
                     QDPDVILVGEMRDLETVATALTAAETGHLILSTLHTSDVAQAVHRIVDVFPPGQQTQI
                     RQQLSLSLNAIVVQQLLPASSGNGRAVAVEVLLANAAVRNHLRRDKMENLISEITLGK
                     RQGMISIEDSLARLVRQGVISVEDARMRSARPDELESQLRT"
     gene            1230..2483
                     /locus_tag="VNN25_25860"
     CDS             1230..2483
                     /locus_tag="VNN25_25860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95026.1"
                     /translation="MALSTRERIDLIVVGIVSLVTNFLYFAYAAPDYFFPDSFTYIEP
                     ARNLLHGLGFTTGDGIIETMRTPGYPLLLTAFGLRIVPVIVFQHLLNAALAVAIYLFV
                     ARRIGSRFVALTAAVLFAIDTPTLHYANKILTESLFTALLFVVFVLVAYNRKLPLAGV
                     LTGVLVLIRPVAIVYFIVLALCLGINRVRMRTIASFIALALVLPIGWELRNLHHTGVF
                     TVSSIGGMNMLSYRAAGALAIEDDGDFDADLVDEQKGLVGDADAEIQAKLHIQDAEDL
                     PDAVRSKYYSQIAWRVLRQHPRAAAMLTLRGLLVNLFDSRWEGMEVVSRFPAMIVQHG
                     LDAFTAIVFTFAAIGAAALWRRDRNLALLLVATIAYYVLISAGGESESRFRVPIMPQL
                     LIAAACGVDAVKRALGVKNSGLPLV"
     gene            complement(2831..>3145)
                     /locus_tag="VNN25_25865"
     CDS             complement(2831..>3145)
                     /locus_tag="VNN25_25865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95027.1"
                     /translation="VERLRAQYMFSPKMFLRAVVQNQRTSSNPLIYGVDMPLRAGTLS
                     SQILFAYKVNWQSVLYFGYGDIRDVNEEVNNTDPANRVYKRNFLLSNRQVFFKMSYAF
                     QK"
BASE COUNT          560 a          999 c          975 g          611 t
ORIGIN      
        1 ccggatgcgt cgaagggaac gtggccggcg gcgcaagtgc cgagcggcaa ccctctcgaa
       61 gcgcttctac tcgacgcggc gcggagaggc gccagcgatc tgcacatcat cgcgggctcg
      121 gtaccggtct ttcgtgtcgg cggacggctc gtacgtggcg aaggctccgt cctctccgac
      181 gatgacgtcg ccccgcttct cgacccgctg ctgactccgc gggtacgcga gcgcatcgcc
      241 gaagacggcg cgatcgattt ctcgctacgt ctggacgcgc cggcggtaag cgaggaacgg
      301 cgcgcgtggc gctttcgcgt gaacattcat cgccagcgcg gcacattcgc cgcatccatc
      361 cgctcgctgc ccaatgcggt tcccacgatt gcggaattga accttccgcc gattctcgat
      421 gaactggtga aaccgacgcg cgggctggtg ctgatctgcg ggccgaccgg cgcgggaaaa
      481 acaaccacgc tggcagcgat gatcggcgcg atcaatcgcg cggagtcgag gcacatcatc
      541 acgatcgagg atccgatcga gtacgagcac cgcaacgcgc agtcggtcat cgagcagatc
      601 gaagtagggc gcgacgcccc ttcgttcgcg tcggccttac gcgcggcgct gcggcaggat
      661 cccgacgtga ttctggtcgg tgagatgcgc gacctcgaaa ccgttgccac cgcgctcacg
      721 gccgcggaga cgggacacct catcctctcg acgctgcaca cgagcgatgt ggcgcaggcg
      781 gttcaccgca tcgtcgacgt cttcccgccg ggacagcaga cgcagatccg gcagcagctt
      841 tcgctcagcc tcaacgccat cgtcgtgcag caattgcttc ccgcctcgtc cggcaacggt
      901 cgtgcggtgg cggtggaggt gctcctggcc aatgccgcgg tccgcaatca cctccgccgc
      961 gacaagatgg agaacctgat ctccgaaatc accctcggca aacgccaggg catgatctcg
     1021 atcgaggatt cgctggcgcg gttggtgcgg cagggggtga tctcagtcga agacgcgcgg
     1081 atgcggtcag cgcggccgga tgagttggaa agccagttgc ggacgtaggt tgatccgcag
     1141 atgtcgcaga tcgacgcaga tggagatcgc agcagctctc atctgcgttt atctgcgtca
     1201 tctgcggatg ggctctagaa tgcgcggcca tggcactgag cacgcgggaa cgaatcgatc
     1261 tcatcgtcgt cgggatcgtt tcgctggtga cgaattttct ctacttcgcc tacgccgcgc
     1321 ctgactattt cttccccgac tcgttcacct acatcgagcc agcccgcaat ctccttcatg
     1381 gactcggctt cactaccggc gacggcatca tcgagaccat gcgcacgccg ggctatccgc
     1441 tgctgctgac cgcgttcgga ttgcgcatcg tgccggtgat cgtcttccag catcttctga
     1501 acgccgcgct cgccgtggcc atctatctct tcgtcgcgcg ccgcatcggt agccggttcg
     1561 tcgcgctgac ggcggcagtt ctcttcgcca tcgacacgcc gacgctgcac tacgcgaaca
     1621 agatcctgac ggagtcgctt ttcacggcgc tgctgttcgt cgtcttcgtg ctcgtcgcgt
     1681 acaaccgaaa gctgccactg gccggagtgc tcaccggcgt cctcgtgctg atccggccgg
     1741 tagcgatcgt ctacttcatc gtgctcgcgc tctgcctcgg catcaaccgc gtccgcatgc
     1801 ggacgatcgc ctcgttcatc gctctcgcgc tcgtccttcc gatcggatgg gagcttcgca
     1861 atctgcatca cacgggcgtc ttcacggtct cgtcgatcgg cggcatgaac atgctctcct
     1921 accgcgccgc cggagcgttg gcgatcgaag atgacggcga cttcgatgcg gatctcgtcg
     1981 atgaacagaa agggctcgtc ggagatgcag acgccgagat tcaggcgaag ttgcacattc
     2041 aggatgcaga ggatctgccc gatgccgtgc gcagcaagta ctactcgcag atcgcgtggc
     2101 gggtgttgcg gcagcatccg cgcgccgcgg cgatgctcac gttgcgcgga ctactcgtga
     2161 atctattcga cagccgctgg gagggaatgg aggtggtcag ccgttttcca gcgatgatcg
     2221 tgcagcacgg actggacgcg ttcaccgcta tcgtcttcac cttcgccgcg atcggcgcgg
     2281 ccgcgctgtg gcggcgcgat cgaaatctcg ctctcttact ggtggcaacg atcgcctact
     2341 acgtgctgat ctccgcggga ggcgaatcgg agtcacgctt tcgcgtaccg atcatgccgc
     2401 agttgttgat tgcggcagcg tgtggcgtgg acgcagtaaa gcgcgcgttg ggagtgaaga
     2461 actctggcct accgctcgtg tgacatctca ggaccagcgg ggttgtgtca tcctgagaca
     2521 gccggagccg ccagctgcga tcgagccctc gcgcgacgtg gagtggcggc ggaggacggc
     2581 gaaggatctc cgccgcgaca atcgtggcag tccagcgcga gcgagtgtgg acaatgacgt
     2641 tcgctcgcgc tagtcaaaca cgatcttcga attttgagat ccttccacgt cctccggcac
     2701 ttcgtgtcct acggcagtct caggatgaca cgatctcggg gcggagagtc agcaagtgat
     2761 acccgcccga aactcgccac cgacatctca ctcccccaca ccccacaccc cacccccaac
     2821 tcccactccc ctacttctga aacgcgtagc tcatcttgaa gaacacctgc cggttggaca
     2881 ggaggaagtt gcgcttgtag acacgattgg ctggatcggt gttgttgacc tcttcgttta
     2941 cgtcgcggat gtcgccgtag ccgaagtaga ggacgctctg ccagttcacc ttgtaggcga
     3001 agaggatctg cgatgaaagc gtgcccgcgc gaagaggcat gtcgacgccg tagatgagcg
     3061 ggttggagct cgttcgctga ttctgcacga cggcgcggag gaacatcttc ggagaaaaca
     3121 tgtactgcgc gcgaagacgc tcgac
//
LOCUS       DATLVW010000879        14054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_285899, whole genome shotgun sequence.
ACCESSION   DATLVW010000879 DATLVW010000000
VERSION     DATLVW010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285899"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VNN25_25870"
     CDS             <1..258
                     /locus_tag="VNN25_25870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95028.1"
                     /translation="MLQNKLEGMVYTNEKVFREFGKMLSDEDRDKVQKIITKSKEQVT
                     SEERQRLNDAIFELQAASRILTSVMLYNPMKMNSPSEPTNQ"
     gene            262..1380
                     /gene="dnaJ"
                     /locus_tag="VNN25_25875"
     CDS             262..1380
                     /gene="dnaJ"
                     /locus_tag="VNN25_25875"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXH95029.1"
                     /translation="MATTEKRDYYEVLGIARTAAVDDIKKAYRRLAVKYHPDKNPGDA
                     DAEEKFKEAAEAYGVLSDDEKRARYDRFGHQAVGGGGFDPNQFADFGDILGDLFGFGD
                     FFGTGRKRTTRAARGNDLRYDLTLEFEEAVFGKDVTIDVPRVATCNVCHGSGAKAGTQ
                     PVTCTGCAGRGQVRYSQGFFAVARTCPQCGGAGKVIKDPCASCDGAGRVREEKTISVK
                     IPAGVDDGSRLRVAGEGEAGFNGGPSGDLYVFLSVREHATFTRRDYDIHSEQTISFTQ
                     AALGADLQTATIEGTEPLRVPPGTQPNQIFRLRNKGVQFLQGPGRGDHYVHVSVRIPT
                     TLDEAHRDLLTRYAELEGETPLQDKGVFGKVKDFFTAQ"
     gene            1762..2379
                     /locus_tag="VNN25_25880"
     CDS             1762..2379
                     /locus_tag="VNN25_25880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HXH95030.1"
                     /translation="MAEQSFSRWRPHPWHGLPAGPDVPSIVNAYIEITPFDLIKYEVD
                     KASGYLRVDRPQRTSSQPPALYGFIPRTYCGPRVAALTPVTKRGDGDPLDICVLSERP
                     IAKSEIILSARVIGGLQLVDRDEADDKIIAVLQGDYVWGDAHDLSDVPSVLIERLEHY
                     FATYKLVPGGPVNIRISGSYGFAHAHAVVQAAMEDYAELYGEPKE"
     gene            2376..3215
                     /locus_tag="VNN25_25885"
     CDS             2376..3215
                     /locus_tag="VNN25_25885"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXH95031.1"
                     /translation="MKDFILDVSFDARDLALEDLVQSRLFLTQSTGSSSSEANGTSIV
                     SAYFDSAHDRDAAAAELRDIEGIELRTTERDRIDWLEHYQQSLEPLFIGEHFVVAPDA
                     SLIPRDTKRRALVVPQEQAFGTGSHETTSLCIEILETMDVAGKYGLDIGAGSGILALA
                     MCRLGGQQVIAFDNDVDAFAALRENRARNEVEPERMPLFIGGTDALRGGAFDIVTMNI
                     IPEVIIPLLGDVKRHVVGSLILSGILVIKRDDVVAACAEHGLSIVDERSKGEWWAGVF
                     RSS"
     gene            3405..4565
                     /locus_tag="VNN25_25890"
     CDS             3405..4565
                     /locus_tag="VNN25_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXH95032.1"
                     /translation="MNQISDRLRGLVQSDIRRMSRECERVGGINLGQGICDLPTIPEL
                     IDGACDAIRANKATYSKFEGIDLLRERIARKVERHNGFTADPAKEIVVTVGSSGGFLA
                     AAMATLNVGDEVILFEPYYGYHLNTFKVLGFEPKFVPLQAPDWTIDFDVLRAAFSKKT
                     KAIVVCTPSNPSGKVFTREDLEKIGELCREFGTWIFTDEIYEYIVYDGRKHVSVASIP
                     SCRDLAITISGFSKTFSITGWRIGYVVADERVASAIGLMNDLFAICAPTPLQWGVARA
                     LEIGDDYYLQMAADYEKKRDLLADALSEAGFTPSVPQGAYYMLATIPDDFRDDKEAAQ
                     ALLDNTRVASVPGSAFFVSDAGKRMLRFCFAKDFASLEEACNRIRAFKPAAV"
     gene            4657..5097
                     /locus_tag="VNN25_25895"
     CDS             4657..5097
                     /locus_tag="VNN25_25895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH95033.1"
                     /translation="MSYLTAWPAREPVTKAHQGLTREWWDEKRNRFDLFISEIVLDEA
                     ARGDQMAAAARLAVLESFPILRVTEQARELAAAILRSTALPPKAAADAMHIAVATVNA
                     MDFLLTWNCTHIANAIVFRTVATVCREMGFEPTTVCTPEELMEG"
     gene            5101..5298
                     /locus_tag="VNN25_25900"
     CDS             5101..5298
                     /locus_tag="VNN25_25900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95034.1"
                     /translation="MHEDPIVAETRKLREELMNEVGNDLDRLFDYLREREDQHAERLV
                     SFAPRRVVAMRGVIADRKLED"
     gene            complement(5807..9091)
                     /locus_tag="VNN25_25905"
     CDS             complement(5807..9091)
                     /locus_tag="VNN25_25905"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HXH95035.1"
                     /translation="MHFFRSQTALAAAGLFTVAILFSVPNAQAATFSVINTLDSGAGS
                     LRQAITDANATAGFDTIVFAIPGAGVHTIAPTSNLPPITEGVFIDGYSQAGSSANTLA
                     LGDNAVLQIEVTGTAISNGIGFSLTGTVTSGEIRGLLITKWNNAIQMSGTTNFTIDGN
                     FFGTNATGTATSSATANGSAFFILNSSANNTVGGATNAARNIISGSTTAGITMGNSGT
                     TGNVIQNNYIGTNAAGTGALPNLQGISLNGADANTIRGNVISGNNTSATFSAGISIIN
                     TSNHILVVGNKIGTTADGTAPLPNTYGITLSDGFSGGATSTTIGTQAEPNTIAFNTKA
                     GVALQAFTSKVSRLNTIRYNSIYSNGTLGIDLGDDGITPNDSGDGDTGLFNDLQNFPV
                     LTSAQFGAGTLTIGGSLNSMPSSAYNVQFFQSTACDPSGAGEGQTFLGETTVNTDASG
                     IGTFNVNFPGTAAGVVTAIATDSLGNTSEFSACQTITTPALPQISISDAQLVEGNAGT
                     SLMTFNVILSAASAATVTVNYATADQTATAGSDYVAGSGTVTFSPGQTTKPVTVTING
                     DVTAEADETFLVNLNTPVNATILDGQGVGTIKNDDNLSALSINDVTLAEGNSGTTSFT
                     FTVTLTPAAAFPVTVNYATADGTAIAGSDYQPASGSLTFTAGQTTKTVTVLVNGDVTT
                     EPNETFVVNLFNPGNASISDAQGLGTITNDDANPSITINDPSVVEGNSGSKSMNFVIT
                     LSNPTSTQLSVPFSLADGTANVGTDYQTNSGSFSVFAGNVTATVSIGINGDTTVEPDE
                     TFFMNLGSVAGATIAKPQGTGTIINDDGLASPGISISDVTASEGNSGTTNFNFAVSLT
                     ASSASTITVNYATADGTATAPSDYTAKSGVVTFAPGQTSQSVTIAVNGDTLFEPNETF
                     FVNLTSPANAVIIDSQGQGTINNDDVVGSADLSILKTGATTAAGSANVVYSIVVTNNG
                     PTGASAVTVTDVLPAGTTFVSAQTTQGVCSGTTTVICALGALNNGSNATITLTIQMPA
                     ASGVVSNTATVTAAENDPTPANNTSTAATTVSGASPPSPVPAQSTLLLLMLGVLLGTL
                     AVIRLR"
     gene            complement(9833..10345)
                     /locus_tag="VNN25_25910"
     CDS             complement(9833..10345)
                     /locus_tag="VNN25_25910"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXH95036.1"
                     /translation="MAEGRMGQDLTRSTPSRHMLHSPMKMTRLIAALNTTLLLLAASL
                     SASPEQDVRAAVLHYRQALVKKDLPTLEQIWTDDYISINSHGVIRTKAERLADLKSGA
                     TSVESIRHEGDIAVKIHGDVAIVTSQITLAGRYNGKEVSGEFRSTHIWKNDKGHWQLL
                     MNQLTAIAKD"
     gene            complement(10428..11228)
                     /locus_tag="VNN25_25915"
     CDS             complement(10428..11228)
                     /locus_tag="VNN25_25915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95037.1"
                     /translation="MKRIIVIIASLLIATAAIAQEKSADKSSDEYNTTRELQSKVFEV
                     HNRDAREIAASVKLLGSGFKGTALSVNEDLHTITARDFKENIVAIESAIARLDHPVAD
                     TPDIELKISVLIGSKTPLAGAESIPEELAPVVKQLQSTLRYAHYGLMTAVVQRTKPGN
                     GINGSGVAEPTLLGMTVREGQPILYTYRLVRITTTDSEHPSIDIQNFEFSMRVPITVG
                     GAQPIQYQSVGFETPVSVRQNEKVVIGTTTMGDKALIVVVTATVAGKG"
     gene            complement(11225..11725)
                     /locus_tag="VNN25_25920"
     CDS             complement(11225..11725)
                     /locus_tag="VNN25_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95038.1"
                     /translation="MKMTEFRSTHPLSDDDLSAIRAKVMAKVAQNQRRGIPLFARLAV
                     AALLVIAVGISFVVRRQTPAAVATSNPQIIRPPHGTPAAPHMNTTIETARAAPPSNAV
                     QSARSSIPKAAARQPVARAVYRPKHRPPQHPEYQTIRMEIRTPDPDVRIIWIANQTPT
                     TTGGNS"
     gene            complement(11722..12333)
                     /locus_tag="VNN25_25925"
     CDS             complement(11722..12333)
                     /locus_tag="VNN25_25925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH95039.1"
                     /translation="MTAEALLTMDVTAVPRTSETAPALLAAIRLARDGNERAFEEIML
                     ASQHRVALLSWRILGDAEEVKEATQETFLRVFRHLHGYDEQQNFFGWLFRVTVNVCRD
                     LEKKRRRRWIFAPFDSALSIASSARAADEDLATKDEVALLTRAIDALPAKERLAIILR
                     DVEELPTDEVAAILGSTPSTVRVQISKARAKLRVWIEARGGSR"
     gene            complement(12462..12950)
                     /gene="bcp"
                     /locus_tag="VNN25_25930"
     CDS             complement(12462..12950)
                     /gene="bcp"
                     /locus_tag="VNN25_25930"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXH95040.1"
                     /translation="MQDFYEPKVGDVAPDFRLPSTRGKEMTLKEHKGKDVILYFYPKD
                     DTPGCTAEACSFRDHESDLSKAHAVVLGVSTDSLESHDKFRGKYELNFPLLSDSTGDI
                     VKMYGVWKEKNMYGKRYWGVARTTFWIGPDGKIKKIWKKVDTKRHAEEVLAALDEAHA
                     KK"
     gene            complement(13079..>14054)
                     /locus_tag="VNN25_25935"
     CDS             complement(13079..>14054)
                     /locus_tag="VNN25_25935"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HXH95041.1"
                     /translation="GYRLAIVNPQTLVGAEIQSILRERAFPFAKVVLLDSSGTATGLL
                     TEIGDEPAVVLPVSDDEMEDCDVVFFCGPAEKNREWIERYDDDNFVAIDLSQPSSAAE
                     GKLAIAGVNLDEIDAQDRLLISPHPAAIPIALILHQIERISRVESCTATVVQPASESG
                     QLGVEELARQTISVLNIQTVPQEVFDRQLAFNLYPAPERNEEYIASQIRALTDPTTQL
                     ALLMTQGTIFHSHTFSLFVRTKDDLSVERITTSLRENAAIAFAEGDQAFGTIDAAGKD
                     EVLIAEVRPDASIRGGFWVWAVCDNLRRSSALNAVLIAEKVLFGSGVN"
BASE COUNT         2537 a         4134 c         4539 g         2844 t
ORIGIN      
        1 atgttgcaga acaagctcga gggcatggtt tacacgaacg agaaggtctt ccgcgagttt
       61 ggcaaaatgc tcagcgacga agaccgcgac aaagtccaga agatcatcac caaatcgaaa
      121 gagcaggtga cctcggaaga aaggcagcgt ctgaacgacg ccatcttcga gttgcaggcc
      181 gcgtcacgca tcctgacgag cgtgatgctc tacaacccga tgaagatgaa cagtccgagc
      241 gaaccgacca accagtaaac gatggctacg acagaaaaac gcgactatta cgaagtcctc
      301 ggcatcgccc gcacggccgc cgtcgacgac atcaagaagg cataccgccg gctcgccgtg
      361 aagtatcacc ccgacaagaa tccgggcgat gcggatgccg aggagaagtt caaggaagcg
      421 gccgaagcgt atggcgtgct ttccgatgac gagaaacgag cccgctacga tcgcttcggc
      481 caccaggccg ttggtggcgg cggcttcgat ccgaaccagt tcgccgactt cggcgacatc
      541 ctcggcgacc tcttcggatt cggcgacttc ttcggcaccg gccgcaagcg caccactcgc
      601 gcggcgcgcg gcaacgatct ccgctacgac ctcaccctcg aattcgagga agcggttttc
      661 ggcaaggatg tgacgatcga cgttccccgc gttgccacgt gcaacgtgtg ccacggcagc
      721 ggcgcgaaag cgggcacgca gccggtcacc tgcaccggat gcgcgggccg cggacaagtg
      781 cgctactcgc agggcttctt tgcggtagcc cgcacgtgtc cgcagtgtgg cggcgccgga
      841 aaagtgatca aggatccgtg cgcgtcgtgc gatggcgccg gccgcgtacg cgaagagaag
      901 acgatctcgg tcaagatccc ggcaggcgtc gacgacggct cgcgtctgcg cgtggcgggt
      961 gaaggcgaag caggattcaa cggcggacca tccggcgacc tgtacgtctt cctctcggtc
     1021 cgcgagcacg ccaccttcac gcgccgcgac tacgacatcc attccgaaca gacgatttcc
     1081 ttcacccagg ccgccctcgg cgccgacctg cagaccgcga cgatcgaagg aaccgagccg
     1141 ctgcgcgtcc cacccggcac ccaaccgaac cagatcttcc gcctccgcaa caaaggcgtg
     1201 cagttcctcc agggccccgg ccgcggcgac cactacgtcc acgtctcggt ccgtatcccc
     1261 accacgctcg acgaagcgca ccgcgactta ttgacgcgct acgccgagct cgaaggcgag
     1321 accccgctgc aggacaaggg tgtattcggg aaggtgaagg actttttcac ggcgcagtag
     1381 gggcaaatct tcgacgactc ccatttgtgt catcctgagc cgccgaagcc gccgcaagat
     1441 tcgctgctct cgcacccggc aggcatcggc ggcggaggac ggtcgaagga tctcaaaata
     1501 cgaaggtctc gttcgagcgt ctctgatcac aatcgctcgc gctggacgaa cacgatctgc
     1561 gtagtttgag atccttcgac gtcctccgcc gcctcgcaca cgctgcgcaa agatcgcgat
     1621 ttgcgcgggc ggctccggca gtctcaggat gacacgatgg gtggcactat gtatgggata
     1681 acacgaaggt atgacgcgat gggacggcac gatcggtcgt gagcgccttc gcccttcatc
     1741 gcgcgctaca atccttcccc cgtggccgag caatccttct cccgctggcg tccccatccc
     1801 tggcacgggc tgcctgctgg tcccgatgtg ccgagcatcg tcaacgccta tatcgagatc
     1861 acgccgttcg atctcatcaa gtacgaagtc gacaaagcgt ccggctacct tcgcgtcgat
     1921 cgtccgcagc gaacgtcgtc gcagccgcct gcactttacg gattcatccc cagaacatat
     1981 tgcggtccac gcgttgccgc gctgacgccg gtcaccaagc gtggcgatgg cgatccgctc
     2041 gacatctgcg tgctcagcga gcggcccatt gccaagtccg agatcatcct ctccgcgcgc
     2101 gtgatcggcg gattgcagct cgtcgatcgt gatgaggcgg acgacaagat catcgccgtg
     2161 ctgcagggcg actacgtctg gggcgacgcg cacgacctca gcgacgttcc ctccgtgctc
     2221 atcgaacggc tcgaacatta cttcgcaacg tacaaactcg ttcccggcgg ccccgtgaac
     2281 atccgcatct cgggctcgta cggtttcgcc cacgcacacg cggtcgtgca ggcggcgatg
     2341 gaagactacg ccgagcttta cggcgaaccg aaagagtgaa ggacttcatc ctcgatgttt
     2401 cgttcgatgc gcgcgacctc gcgctcgagg atctcgtgca gtcgcgattg ttcctcacgc
     2461 aatcgaccgg cagctcctcg tccgaggcaa acggcacgtc gattgtctcc gcctacttcg
     2521 attcggcgca cgaccgggac gctgccgcgg cagagttgcg cgacatcgaa ggcatcgagc
     2581 tccgcaccac cgagcgcgac cggatcgatt ggctcgagca ctaccagcaa tcgctcgagc
     2641 cgctcttcat cggcgagcat ttcgtcgtcg ctcccgatgc ctcgctgatt ccgcgcgata
     2701 ccaaacgccg cgcgctcgtc gtgccgcagg agcaagcctt cggcaccggt tcgcacgaaa
     2761 cgacgtcgct ctgcatcgag atcctggaaa cgatggacgt cgccggaaag tacggtctcg
     2821 acatcggcgc cggcagcggc atcctcgctc tcgccatgtg ccgcctcggc ggacaacaag
     2881 tcatcgcctt cgacaatgac gtcgacgcct tcgcagcact gcgcgagaac cgcgcccgca
     2941 acgaagtcga gcctgaacgg atgcctcttt tcatcggagg aacagacgcg cttcgcggcg
     3001 gcgcgttcga catcgtcacg atgaacatca tccccgaggt catcatcccg ctcctcggcg
     3061 acgtgaagcg gcatgtcgtc ggatcgctga tcctcagcgg gatcctcgtc atcaagcgcg
     3121 atgacgtcgt cgccgcttgc gcggagcacg gactgtcgat cgtcgatgag cgatcgaaag
     3181 gggagtggtg ggcgggagtg ttccggtcgt cgtgattcga aagcacagac aagagtgtct
     3241 gtgccacacc actcctgctt cgtgcccggg gctttcgtat cgccagctca atgtgacagg
     3301 cactctgtct gtgccacaga cacctgcttc tttgctcagg ccttcctgtc acgcagctca
     3361 atgtggcaca gacacttttg tctgtgcttt aaactatccg caccatgaac cagatcagcg
     3421 atcgtctccg cggccttgtg cagtccgaca tccgccgtat gtcccgcgag tgtgagcgcg
     3481 tcggcggcat caacctcggg cagggtatct gcgatctgcc cacgattcct gagcttatcg
     3541 acggcgcttg cgatgccatc cgtgcgaaca aggcgaccta ctcgaagttc gaagggatcg
     3601 acctgcttcg cgaacgcatc gcgcgaaagg tcgagcgtca caacggattc actgccgacc
     3661 cggcaaagga gatcgtggtc acggtcggct cgagcggcgg tttcctcgcc gcggcgatgg
     3721 caacgttgaa tgttggcgat gaagtgatcc tcttcgagcc gtattacgga tatcacctga
     3781 acacgttcaa ggtcctcggc ttcgaaccga aattcgttcc gctgcaggcg ccggactgga
     3841 cgatcgactt cgacgttctg cgcgccgctt tttcgaaaaa gaccaaggcg atcgtcgtct
     3901 gcacgccgtc gaatccgtcg ggaaaagtgt tcacgcgcga ggatttagag aagatcggcg
     3961 agctgtgccg cgaattcggc acctggatat tcacggatga gatctacgaa tacatcgtct
     4021 acgacggccg gaaacatgta tcggtcgcct cgatcccatc ctgccgcgat ttggccatta
     4081 cgatcagcgg cttctcgaaa acgttctcga tcaccggctg gcggatcggc tacgtcgtcg
     4141 ccgacgagcg cgtcgcaagc gctatcggcc tgatgaacga tctcttcgcg atttgcgcgc
     4201 cgactccgct tcaatggggt gtggcccggg cgctggagat cggcgacgac tactatctcc
     4261 agatggcagc cgactacgag aaaaaacgcg acctcctcgc cgacgcgctt tccgaggccg
     4321 gctttacccc ctcggtgccg caaggcgcgt actacatgct cgccacgatt cccgatgatt
     4381 tccgcgacga caaggaagcc gcgcaggcac ttctcgataa cacccgcgtc gcctccgtcc
     4441 ccggctcggc cttcttcgtt tcagacgcag gcaaacgcat gctccgcttc tgcttcgcga
     4501 aagatttcgc gtcgctcgaa gaagcgtgca accggatccg cgcgttcaag ccggcagcgg
     4561 tgtaaggcga gccgcgaatc acatcggctt ctccgcacga cccatttggg ttagggttgc
     4621 cctgtgcgac cgaaggctta ccttgaaacc acaatcgtga gctacctgac tgcgtggccg
     4681 gcgcgcgagc cagttacaaa ggctcatcag ggacttactc gcgagtggtg ggacgagaaa
     4741 cggaaccgct tcgacttgtt catctcggag atcgttctgg atgaggcagc gcgtggagat
     4801 cagatggcgg ccgcggcgag gctcgcagtc ctggagtctt ttccgattct gcgcgtcacc
     4861 gaacaggcaa gagaactagc ggcagctatt ctgcgttcga cagcattgcc accaaaggca
     4921 gcagccgacg ccatgcacat cgccgttgct accgtaaacg cgatggactt cctgctgacc
     4981 tggaattgca cgcacatcgc caacgcgatt gtttttcgca cggtggcaac ggtctgtcgt
     5041 gaaatgggat ttgagcccac gactgtttgt acgcccgagg aactgatgga aggctaaacg
     5101 atgcacgaag atccgatcgt cgctgaaact cgcaaattgc gcgaggaact gatgaatgag
     5161 gtcggcaacg atctcgacag gctgttcgac tatcttcggg aacgcgagga ccagcacgca
     5221 gagcggctgg tgagctttgc gccgcgccgc gttgtggcca tgcgtggcgt catcgcagac
     5281 agaaagctgg aagactagtc tcacgtccaa cttgaaaacg tgggtcgcct ccgctcctgc
     5341 agatcgcgtt caagggcggc cagctgtacg cgtcggcacc gcagccttcg cagatcgtat
     5401 tcacgcgcct gcaggcaact gacgatttca actgtacttt ttcgttctgg cgaacggaga
     5461 gtggtgggga ccaaagggct taccgtgcgg accggagggt tttccaagcg ggcccttggt
     5521 ttaaccgtgc gggccggggg ttttaccgtg cgggccggag gtttgacggt gcgggccggg
     5581 ggtttgacgg tgcgggccga cggtttgacg gtgcgggccg gaggtttgac ggtgcgggcc
     5641 ggaggtttaa cggtgcgggc cggcggttta accgtgcgga ccggaggttt taccgcgcag
     5701 accggaggtt tcaccgtgcg gaacctgcga tggcgaagac agccagcctt ggcaataaga
     5761 aaaaggcggc cagacggccg cctttcttgc caagcatgtg ctgaagttac cgcaagcgga
     5821 tcaccgccag cgttcccagg aggacgccga gcatcagcag cagcagtgtc gactgtgccg
     5881 gcacagggct cggcgggctc gcgccgctca ccgttgtcgc tgcggtggat gtgttgttcg
     5941 ccggcgttgg gtcgttttcg gcggcggtca cggtcgcggt gttcgagacg acgcccgacg
     6001 ctgcgggcat ctggatggtc aacgtgatcg tggcattgct gccgttgttc agtgcaccta
     6061 gggcgcagat taccgtcgtg gtgccgctgc agacgccctg ggtcgtttgt gccgacacga
     6121 acgtcgttcc cgcaggcagg acatctgtga ccgtcacagc cgacgcaccg gtgggaccgt
     6181 tgttcgtcac gacgatgctg tagacgacgt tcgcgcttcc ggctgcggtt gtggctcccg
     6241 tcttgagaat agaaagatcg gccgagccga ccacgtcgtc attgttgatc gttccctgac
     6301 cctggctgtc gatgatgact gcgttggcgg gagatgtgag attgacgaag aaggtctcgt
     6361 taggctcgaa gagcgtgtcg ccattcacgg cgattgtcac gctctgcgat gtttggccgg
     6421 gagcgaacgt aacgacgccc gacttcgcgg tgtagtccga cggcgcggtg gcagttccat
     6481 cggctgtggc gtagttgacc gtgatcgtcg aggccgagct ggcggtgagc gaaaccgcga
     6541 agttgaagtt cgtcgtgccg gagttgcctt cgctggccgt cacatcactg atcgagatgc
     6601 cgggcgaggc cagaccgtcg tcattgatga tcgtgccggt tccctgtggt ttggcgatcg
     6661 tggcgccggc caccgagcct aggttcatga agaaggtctc gtcgggctcg acagtggtgt
     6721 cgccgttgat cccgatcgaa accgtggccg tgacattacc ggcgaagacg ctgaaggagc
     6781 cgctattggt ctggtagtcg gtcccgacgt tggccgttcc gtcggcgagg ctgaagggaa
     6841 cggaaagctg agtggaggtc ggattcgaca gcgtgatcac gaaattcatc gatttcgaac
     6901 cgctattgcc ttcgacgacg gaagggtcgt tgatcgtgat cgatggattg gcatcatcgt
     6961 tcgtgatcgt tcccagcccc tgcgcgtcgc tgatcgaagc gttgcccgga ttgaagagat
     7021 tgacgacgaa tgtttcgttc ggctccgtcg tcacgtcgcc gttcacgaga acggtgaccg
     7081 tttttgtggt ctgtcccgcg gtgaacgtca gcgaaccgct ggcaggctgg tagtcgctgc
     7141 cggcgatcgc ggttccgtca gcggtcgcgt agttgacggt gacagggaac gccgccgccg
     7201 gagtgagcgt gacggtgaag gtaaagctgg tcgtgccgga gttgccttcg gcgagcgtga
     7261 cgtcattgat cgagagcgct gaaaggttgt cgtcgttctt gatggtgccg acgccctgac
     7321 catcgaggat cgtggcgttg accggcgtgt tgaggttcac gaggaaggtc tcgtccgcct
     7381 cggcggtcac atcgccgttg atcgtgaccg tgacgggctt cgtggtctga ccggggctga
     7441 aggtgaccgt gccgctcccc gcaacgtagt cggagcccgc ggtcgccgtc tgatcggccg
     7501 tcgcatagtt gacggtcacg gtcgccgccg acgctgccga gagaatgacg ttgaaggtca
     7561 tcagggacgt gccggcgttt ccttccacca actgcgcgtc actgatgctg atctgcggca
     7621 gggccggtgt ggtaatcgtc tggcacgcgg agaattcgga ggtgttgccg agggagtcgg
     7681 tggcgatggc ggtgacgact ccggcagcgg tcccggggaa gttcacgttg aaggtgccga
     7741 tgccggatgc atcggtgttg acggtggtct cgccaaggaa ggtctggccc tctcccgcgc
     7801 cggatggatc gcacgcagtg ctctggaaga actgaacgtt gtaggcactg gaaggcatgc
     7861 tgttcagaga gccgccgatc gtcagcgttc ccgcgccgaa ctgggcggag gtgagcaccg
     7921 gaaagttctg cagatcgttg aacaggcccg tgtcgccgtc gccggagtcg ttcggcgtga
     7981 tgccgtcatc acccaggtcg atgccgagtg tgccgttgct gtagatggag ttgtagcgga
     8041 tcgtgttcag gcgtgagacc ttcgatgtga aggcctgcag ggctacgccg gctttcgtgt
     8101 tgaacgcgat cgtgttcggc tcggcctgcg tgccgatcgt ggttgacgtg gcgccgcccg
     8161 agaaaccgtc ggagagcgtg atgccgtacg tgttgggaag cggcgccgtg ccgtccgcgg
     8221 tcgtgccgat cttgttgcca acgacgagga tgtgattcga ggtgttaatg atggagatgc
     8281 cggccgagaa cgtagcgctg gtgttatttc cggagatcac attgccacgg atggtgttgg
     8341 catcggcacc gttgagcgag atgccctgaa ggtttgggag agcgcccgtg ccggcggcgt
     8401 tcgtgccgat gtagttgttc tgaatgacat tgccggtcgt gccgctgttg cccatcgtga
     8461 tgcccgcggt cgtgcttccg gagatgatgt tgcgcgcggc gttggtggcg cctcccaccg
     8521 tgttgttcgc ggaggagttg agaatgaaga acgcgctgcc gttggcggtc gcggatgacg
     8581 tcgccgtgcc ggtggcattc gtgccgaaga agttgccgtc gatggtgaag ttggtggtgc
     8641 cggacatctg aatggcgttg ttccacttgg tgatcagcaa cccgcggatc tcgccgctgg
     8701 tcacggtacc agtgagactg aagccgatgc cgttggagat ggcggtaccc gtcacctcaa
     8761 tctgaagaac ggcattgtcg ccgagggcca gggtattggc gctcgacccg gcttgcgagt
     8821 agccgtcgat gaagacgccc tccgtgatgg gcgggaggtt gctcgtgggc gcgatcgtgt
     8881 gcacgccagc ccccggaatc gcgaagacga tggtgtcgaa tcccgccgtc gcattcgcgt
     8941 ccgtaatggc ctgacggagc gagcctgctc cggaatcgag tgtgttgatg acgctaaacg
     9001 tagccgcctg cgcgttcggt accgagaaaa gaattgccac agtgaacaga ccggcagcgg
     9061 cgagcgccgt ctgcgaccgg aagaaatgca tgaagttctc ctattgcgac cgtatgaagg
     9121 tgactcaggg tagccggtat ccgggcgccg ctcgtgacaa aggtcaccga ttgacgaaat
     9181 ggagtgcggt cgtctcgacc gcgggcacgc gggcgtcctc gcccgagtgc tgtaagggcg
     9241 gaggtcgaag ttctagcttg cgtgaagtac gcgttggcgg ttgcggccgt cgtgttccag
     9301 ttgaaatccc cagtttccct tgagcgcacg cgggacgtga tcgcacatcc tcagttgttt
     9361 gccgggattt cgtgcacgat gacgtcggga tcgcggtggg cgtgtgttcc gctggaatcg
     9421 ttctgtcgcc cgctgaagcg ggcttggctg atgggaactt tcgcgaagac ccccggctga
     9481 agccgggggc tacgatctca tcatcggttg tttgccggag cttcgtgccc gatgagaggg
     9541 atcgcggctg ggcgttttga ccaccagagt caaataccgt ctgaacagaa tcgaacgtgt
     9601 cacggttgtg agatcgtagc ccccggcttc agccgggggt ctcgatgtgg tccccaattt
     9661 gcgaagcccg cttcagcggg cgacagagaa ttggccttgc ggttcccagg ctgaagcagg
     9721 gctgcgatct gctgggatca cgcctgcaac cgccagttgg cgttgaagtg cacgctggtg
     9781 aacgcgatct gtgcgggccg aggcgactca cgttttcaag atggacgtga cgctagtcct
     9841 tcgcaatcgc cgtcaactgg ttcatcagca actgccagtg acccttatcg tttttccaga
     9901 tgtgcgtgct gcgaaactcg cccgagacct ccttgccgtt gtaacgaccg gcgagggtga
     9961 tctgcgaggt gacgatagcc acgtcgccgt ggatcttcac ggcgatgtca ccctcgtgcc
    10021 ggatcgattc gacagatgtg gcgccggact tgagatcggc gaggcgctcg gctttggttc
    10081 ggatcacacc gtgggaattg atggagatgt agtcatcggt ccagatctgc tcgagcgtgg
    10141 gcagatcctt cttgaccagc gcctgacggt agtggagcac ggcggcgcgg acatcctgct
    10201 caggcgaggc ggagagggag gcggcgagca acaggagggt ggtgttgagg gcggcgatca
    10261 gtctggtcat cttcataggc gaatggagca tatgacggct gggggtggaa cgcgtgaggt
    10321 cttgccccat ccggccttcg gccaccttct ccccatcaaa aagcgatggg gagaaggcgc
    10381 tcgattcgag ggcgtggcga gtgtgaaaaa gtccgagacc gcgcggccta tcccttcccc
    10441 gccaccgtcg ccgtcacgac gacgatcagc gctttgtcgc ccatcgtcgt tgtgccgatg
    10501 actacctttt cgttttggcg gacggagact ggggtctcga agccgacgga ctggtattgg
    10561 atcggttggg cgccgccgac ggtgatgggg acgcgcatcg agaattcgaa attctggatg
    10621 tcgatcgagg ggtgctcgga gtcggtggtg gtgatgcgca ccaggcgata ggtgtagagg
    10681 atcggctggc cttcgcggac ggtcatgccg aggagggtgg gctcggcgac gcccgatccg
    10741 ttgatgccgt tgcccggctt tgtccgctgc accacggcgg tcatcaggcc atagtgggcg
    10801 taacggagcg tcgactggag ctgcttgacc accggcgcca gctcctcagg gatgctttcg
    10861 gcgccggcga gcggcgtctt cgagccgatc agcacggaga ttttcagctc gatgtcgggc
    10921 gtgtcggcga cgggatggtc gagccgtgcg atcgcgcttt caatggcgac gatgttctcc
    10981 ttgaaatcgc gcgcggtgat cgtgtgcagg tcttcgttga cgctaagagc cgtgcctttg
    11041 aagccgctgc ccagcagctt gacggacgcg gcgatctcac gggcgtcgcg gttgtgaacc
    11101 tcgaagactt tgctctgcag ctcgcgcgtg gtgttgtact cgtctgagct cttatcggcg
    11161 cttttctctt gtgcgatcgc ggcagtggcg atgagcagtg acgcgataat gacgatgatt
    11221 cgtttcatga gttgcctcct gttgttgtcg gggtctggtt cgcgatccag atgatgcgga
    11281 cgtcgggatc cggcgtccgg atttccatgc ggatggtttg gtactcggga tgctgcggtg
    11341 ggcgatgctt cggccgatag acggcgcgcg cgacgggctg gcgcgcagcg gctttcggta
    11401 tcgaagacct cgccgactga acagcgttcg atggcggcgc agcgcgcgcg gtctcgatgg
    11461 ttgtgttcat gtggggagcc gccggcgttc catgaggcgg tctgatgatc tgcgggttgc
    11521 tggtagccac cgcagccggt gtctgccttc taacgacgaa gctgatgccg actgcgatga
    11581 cgagcagcgc ggcgacggca agccgcgcga agagagggat tccgcgccgc tggttttgcg
    11641 cgactttggc cataaccttg gcgcggatcg cggacaggtc atcgtcagaa agcggatgcg
    11701 tcgaacggaa ctcggtcatc ttcatcgcga acctcctcgc gcttcgatcc agacgcgcaa
    11761 cttcgcgcgt gctttgctga tttgcacccg caccgtcgag ggcgtgctgc cgaggattgc
    11821 ggccacttcg tccgtcggta gctcctcgac gtcgcgaagg atgatggcca gccgctcttt
    11881 tgccggcagc gcatcgatgg cgcgcgtgag cagtgcgact tcgtccttcg tcgcgagatc
    11941 ctcatccgcg gcacgggcgc tgctggcgat gctcagggcg gaatcgaacg gcgcgaagat
    12001 ccagcgccgt ctgcgtttct tctccagatc gcggcagacg ttcacggtga cgcggaagag
    12061 ccagccgaag aagttctgct gttcgtcgta gccgtgcagg tgacggaaga cgcgcagaaa
    12121 cgtctcctgc gtcgcctcct tgacctcttc ggcatccccc aggattcgcc acgaaagcaa
    12181 tgcgacacga tgttgggatg cgagcatgat ctcctcgaaa gcacgctcgt taccgtcgcg
    12241 ggcgaggcgg attgccgcca ggagcgccgg cgccgtctcc gacgtcctcg gcaccgcggt
    12301 tacatccatc gtcaacagcg cctcggcagt catcgttcgt gcgttctcaa acaacctcta
    12361 ctcgggtgag gaggagttcg tttacttgga cgcggacgaa tggcgcaggt ttcatctgcg
    12421 aaaggcttta tccgcagatg gcgcagattg acgcagatga actacttctt tgcgtgcgct
    12481 tcgtcgagcg cggcgagcac ctcttccgcg tggcgcttcg tgtcgacctt tttccagatc
    12541 ttcttgatct tgccgtcggg gccgatccag aacgtcgtgc gggccacacc ccagtagcgc
    12601 ttgccgtaca tgttcttctc tttccaaacg ccgtacatct tcacgatgtc gccggtcgag
    12661 tcggagagga gagggaagtt gagctcgtac ttgccgcgga acttgtcgtg gctttccagc
    12721 gagtcggtgg aaacacctag gactaccgcg tgagctttgc tgagatcgga ttcgtgatcg
    12781 cggaacgagc acgcctcggc cgtgcaaccc ggcgtatcgt ccttcggata aaagtaaagg
    12841 atgacgtcct tccccttgtg ctctttcagc gtcatctctt tgccgcgtgt cgacggcaga
    12901 cgaaagtcgg gtgctacgtc gccgactttc ggttcgtaga agtcttgcat aggttctctc
    12961 cttgttagcg gccagtgttg cgcagagaga tcagaggatc agagatcagg aaagaatcag
    13021 cagcgcgcga cgctggtttt cctgatctct aatctcttgt ctctgatctc tgctttactc
    13081 aattcactcc gcttccgaac aataccttct cggcgatgag cacggcgttc agcgcggagc
    13141 tgcggcggag attatcgcag acggcccaca cccagaagcc gccgcgaatc gacgcatccg
    13201 gccgcacctc ggcgatcagc acttcgtcct tgcccgccgc gtcgatcgtg ccgaaggcct
    13261 gatcgccctc cgcgaacgcg atcgcggcgt tctcgcgcag cgacgtcgtg atccgttcga
    13321 cgctgagatc gtctttcgtt cgcacgaaga gcgagaaggt gtggctgtga aagatcgtgc
    13381 cctgcgtcat caggagcgcc agttgcgtgg tgggatcggt cagcgcgcgg atctgcgagg
    13441 cgatgtactc ctcgttgcgc tccggagccg ggtagagatt gaaggccagt tgccggtcga
    13501 acacttcctg cggcacggtc tggatgttga ggacgctgat cgtctgccgg gccagctcct
    13561 ccacgccgag ctgtcccgat tcggacgctg gctgcacgac cgtcgcggtg caactctcca
    13621 cgcggctgat gcgctcgatc tggtggagga tgagcgcgat cggaatcgcg gccggatgcg
    13681 gcgatatgag caaccggtct tgcgcgtcga tctcgtcgag attcacgccg gcgatggcga
    13741 gcttgccttc cgcggcgctg gacggctgcg agagatcgat cgcgacgaag ttgtcgtcgt
    13801 cgtagcgctc gatccattcg cgattcttct cggccggtcc gcagaagaag acgacgtcgc
    13861 aatcttccat ttcgtcatcg gagaccggaa gcacgactgc cggctcatcg ccgatctccg
    13921 tcagcaatcc ggtcgccgtt ccgctgctgt cgagcaggac gactttggcg aagggaaagg
    13981 cccgctcgcg aaggattgac tggatctccg cgccgaccag agtctgcgga ttgacgatgg
    14041 caaggcggta gccg
//
LOCUS       DATLVW010000880        16055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_36167, whole genome shotgun sequence.
ACCESSION   DATLVW010000880 DATLVW010000000
VERSION     DATLVW010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16055
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36167"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..721
                     /locus_tag="VNN25_25940"
     CDS             314..721
                     /locus_tag="VNN25_25940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95042.1"
                     /translation="MIAAPVLAELPPDVYKDMQRKAPEVLFIQVSSVRVHRRFAKPSG
                     CAFFDFEVIREVKVDARILRVARSETRVHAGETIEIEYSSISRCSGWDGPRSIQLLES
                     GDRVYAFLTRRGRTASFEPAARGATFAGSMPGV"
     gene            731..1228
                     /locus_tag="VNN25_25945"
     CDS             731..1228
                     /locus_tag="VNN25_25945"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH95043.1"
                     /translation="MRPSEIIELYRYNDWANHRLLRAASILSTEALAQDLGGSYKSIR
                     DVVAHIVAAEWAWLERWNGRSPESIPDWVSGSLSVLLEHLSDVEAQRNVFLQELSDAA
                     LSSEVAFRFLSGAADHHPLRDLMIHVVDHSSYHRGQLAAKLRQAGAVPPPTGFIAFKS
                     ELRAS"
     gene            complement(1585..3888)
                     /locus_tag="VNN25_25950"
     CDS             complement(1585..3888)
                     /locus_tag="VNN25_25950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH95044.1"
                     /translation="MNDIDRSSVIAPGTRIGPYEVISLLGEGGMGEVYRGRDPRLGRD
                     VAIKILTQDSARDTDAMARFEREARAVASLSHPNILAIFDLGQHEGTRYVVTELLEGE
                     TLRERIHNAPMNWRKSVEIGAEIAEGLAAAHSRSVVHRDLKPENIFLTHEGRVKILDF
                     GLAQVDPFLVAGDQTNQPTTRFKTGPGTVIGTLGYMAPEQLRGESVEPSADIFSLGCL
                     LFEMVTARKPFLRSSGAATIAAILKEDIPRESLSDVVPLELQRIIEGCVEKVPVDRFQ
                     SARDLALTLRAIGSSSSVISGLLNEVERRPGRRSKSIDSIAVLPFQNVGNDPETEYLS
                     EGITEGIINRLSQLPKLKVMARSTVFRYKNREGDAQAVGRELRVRAVVTGRLQHVGGR
                     LVINVELVDAIDGAQLWGERYNRELADLMQLQDEMSREVAEKLRLKLTGAEKKRLRKR
                     STENTAAYQLYLRGRHQWNKRNEESLHKSIAFFREAIDADPSFASAYAGMADSYITLG
                     TNIPLPPREAMPRAQAAARRAVELDPSLAEAWASLAAVKWWFDWDWDGAEADYQRAIG
                     LNPNYSAVHDGFGMLLCARGRYDAASEQFAKAASLDPLSLIVTVHAGWPHYFARQYDV
                     AIAQFEKALELDDHFVPAHGWHGMALSQQGRHDEALQAFERGLAVDRVPILMAMRGHA
                     HALAGHRKQAEEALAALNAESKSRYISPYDLAVIHAALGDTKSAIAALHEALEDHSAW
                     MVFLNIDPRLDSMRSVPVFDALARKLR"
     gene            complement(3991..4302)
                     /locus_tag="VNN25_25955"
     CDS             complement(3991..4302)
                     /locus_tag="VNN25_25955"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXH95045.1"
                     /translation="MKPVRPNDDAEAEFRENIHWYEAESAGLGDRLWSEIQAAISLIS
                     THPSIGEVVPRIRRPRPVRRILLRHFPFFLVYREYTDHIEVVALAHTSRKPNYWRKRL
                     T"
     gene            complement(4302..4535)
                     /locus_tag="VNN25_25960"
     CDS             complement(4302..4535)
                     /locus_tag="VNN25_25960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXH95046.1"
                     /translation="MKDTAALLDQVLSLPENDRAEMATRILESLDPEVQRDVDEAWAV
                     EIERRCAAVDAGTLATSDWKDVRARIERDIFGR"
     gene            4633..5649
                     /locus_tag="VNN25_25965"
     CDS             4633..5649
                     /locus_tag="VNN25_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXH95047.1"
                     /translation="MASHVDFLSADSVSEYPTPPQAPLPAEDDLLDAYSTAVTSAASR
                     ITPSVVNIEVRKPGPRGEERGGGSGFLFTPDGFILTNCHVVAGASSIDVTLIDGTRCT
                     ATVVGSDPHTDLAVIRVSAPNQIPARLGDSDLIRPGQIAIAVGNPLGFQTTVTAGVIS
                     ALGRTMRAQSGRLIDSVIQTDAALNPGNSGGPLVNSRGEVIGVNTAVIRPAQGICFAI
                     GVNMAKFVAARLIRDGHITRSYIGVAGQGVSVHRRLVRFYNLAVESGVLVHSVEKKSP
                     AERSGVREGDIIVGYGDTPVSSVDHLHRILSEEQIGRESTLTLIRSTEKVIVSITPVP
                     AAAQ"
     gene            complement(5664..7067)
                     /locus_tag="VNN25_25970"
     CDS             complement(5664..7067)
                     /locus_tag="VNN25_25970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95048.1"
                     /translation="MRRSTIETLAIAIAIVILLPAARNRPSHPPETPVILNATRSFEI
                     TDKSIVASFTLERVLTQLIERSGVNGLTADQLIRRMFDTQTPRSGAIDPDGPHCDDTL
                     TNGVPSFNGFPRRCPTPEAKLVAVQNLAGQFFTLGLMNRFDQAPPDGSNCGQYRMVFA
                     HRDSETSASTLVRRHLIFEAVLPNPNPALGLAACRPIAKFWADLTAVDSMDERRARLE
                     RFFFQGLDGFAPVVDAANFADPGGIRTLQQSADGGNVSIRFYQFRLAKQCGSAGCTLR
                     FIPDVLENQPFGLLFDGTNTSAQARALRTEFLKHVPTLAIEDLNLMSMRLPKDYLMAE
                     SDTPGTPGVVLFEIAFNSGQSSSDGVAFRANIQSELTLAGSSLTPNNVIVRAEEQSCA
                     GCHGFNGAVPFGGNVKLVIGFQGDQMISEDILADGEAGPSTRYGIDPIIEKQFIPHRI
                     QILSDFLRDGTPPVHSQ"
     gene            complement(7250..7519)
                     /locus_tag="VNN25_25975"
     CDS             complement(7250..7519)
                     /locus_tag="VNN25_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HXH95049.1"
                     /translation="MPDESCTHIGAITAVKHAKKRECEDCVKIGSRWVHLRTCQECGA
                     TRCCDDSPNRHASKHAHATSHPVIASAEPGERWLYCYPDEAFAEY"
     gene            complement(7532..9220)
                     /locus_tag="VNN25_25980"
     CDS             complement(7532..9220)
                     /locus_tag="VNN25_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH95050.1"
                     /translation="MNASDPPAVALEPSASSVQEHLFPTLTDAQVARIAAHGRRRAVA
                     RGDVLVDVAEKVVPFFVVVEGEIEVLRPSRGTETLIVTHRRGQFSGEGTMITGRRSLA
                     RIRVSEPGEVIELDREQLLALIQTDAELSEILMRAFILRRVELIARGLGDVVLIGSAH
                     CAGTLRVKEFLTRNGHPYAYVDLDHDAEAQDLLDRFQVSARDVPVLICRGDAVLRNPS
                     NQRIADCLGFNPAIDTAQLRDVVIVGAGPAGLSAAVYGASEGLDVLVFELNAPGGQAG
                     SSSRIENYLGFPTGISGLELTARAYAQAQKFGAQIMIAKGAAKLTCNRKPYAVQIDDG
                     QRVLTRALIIATGAEYRRPPFENAMQFEGAGLYYGATPMEAQLCVDEEVIVVGGGNSA
                     GQAAVFLAQSVKHVHMMVRAEVLADSMSRYLIRRIEDNPGITLHTRAEIVGLEGERHL
                     ERVRWRDNRNAAVETYDIRHVFMMTGAAPNTQWLEQCVALDDKGFIKTGPELTPDDLV
                     TAGWPLTRPPYLLETSLPGVFAVGDVRGGNIKRVASAVGEGSIAIAFVHQVLHQ"
     gene            9385..9828
                     /locus_tag="VNN25_25985"
     CDS             9385..9828
                     /locus_tag="VNN25_25985"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXH95051.1"
                     /translation="MNKTRWSSLAVAVSLLFAFTAIAQQGGGQQGGPNQTFTQRYGQQ
                     LNLTDDQKKQIDEMDAKFQKNNAEFLESYQKTMAEYREARQANDTAKLDALRPKVDSA
                     RAEMSKLRGGHEEKIAATFNDAQKAQWQKIKEEREARMKERQQHQ"
     gene            9922..10458
                     /locus_tag="VNN25_25990"
     CDS             9922..10458
                     /locus_tag="VNN25_25990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXH95052.1"
                     /translation="MYCVFIHGPAASGKLTVANGLKARSGLPVFHNHLAVDAALSLFE
                     FGSPGFVRLREAVWLAAFREAAERGRSFIFTFSPEASVPPAFIDTAVDVIEGAGGQVL
                     FVSLTCGEAVIEERIEAASRSAFRKLTSLAEYRQLRERGAFAFPPLPADITIATDELS
                     PEDAAGLIQELIGNRGIH"
     gene            complement(10662..12260)
                     /locus_tag="VNN25_25995"
     CDS             complement(10662..12260)
                     /locus_tag="VNN25_25995"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH95053.1"
                     /translation="MTKTLLALLLPILVAVTATAGETAASVALLVNEKDAAAVAVRVA
                     PALAATDAVTRAAASRVALVRGLTGVLPQIHDALMVESDSSAAREEVRALVILGDPAD
                     VDRARAATRKFAPGIDDVIARAVARRTDAFDIYVSLLRAGGFIPDASFFTQALWHRPS
                     AAIAAGSRLLGMRDAAGWRALLAALKDAHLAMQADVLAASLNMPSEEIRTASVWYLVH
                     AYVPDPAMIAPRVRTILSTPTEEASTREAFGRELLRRMLGGEKKDDPRWLEWLQTNEA
                     DPLLATDEALFQHFTEKEFLVRKNHCGVTSYDCRMPERRSAARVIPSTDVAEPAFFLP
                     DLLPPGVASAVVAETHCNGSWLGLAGATADSAGRVQAVTVKRIDMDTACDKAVTTLMR
                     LSLVTPTSLDVPMEASSILLVHGFKQPPCLDEAPLDASNGAPRDVGGEITAPIVKRRK
                     EPEFPQSARHRMVGSAMVSVVLRCIISRDGCVRAVQLVRQSPYPEMNGAALMAISQWT
                     FEPGRLRGVPVDVVFLLTVNFRTN"
     gene            12328..14778
                     /locus_tag="VNN25_26000"
     CDS             12328..14778
                     /locus_tag="VNN25_26000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXH95054.1"
                     /translation="MANRSRIKDFLWRHAAGIASIVMVCFVAFLAFVGYSYVVLTKKF
                     DSSRRWDLPSRIYSDATPIVPGMTIPRSLLEPKLNHVGYHESVSRVTNPGEYRYAGNT
                     LEIYLQNFEYPDIEFRALPVVIQMDGGTVRAIHRLDDGVALRGIRIEPELITSIYNNE
                     MEDRLPIALSAVPKTVSNAIIAVEDKGFYSHRGLSITGILGALRTDVKNRNITHGGST
                     LTQQLIKNLYLNPERTWRRKAIEALYAVLLEARYSKQEILEGYLNEIYLGQNGAVQII
                     GVEQASQVYFGKHVTYLTLPEAATLAGMIHSPNVENPLKYPERAKPRRDLVLSLMRDQ
                     NLITPAEYDAAVASPVTTARFPKTSRSAPFFVDLVLKQLKETHPETQLRTEGLRIFTT
                     LDTIMQRSAEQSLDEGIASLNKHYSYLRNSPTPLEGVMLTIQPGTGYVKALVGGRNYS
                     KTQFNRAIQARRQPGSLFKPFVYATAMDPARGQQAFTAATVLDDSPISVRTGNAVWTP
                     QNYDLKYHGHVTVREALAHSYNIPAVRAAMNAGVPNVIKTASTIGIESPLAPYPSVAL
                     GSFEVTPLEIAYAYSAFANLGVKAEPISILAVSTSEGVLLESREVKMKRVAGSGVSYV
                     MNQILKDVFVYGTGSKARQMGFTRPFAGKSGTTSSYRDAWFIGYSPRILSLVWLGFDD
                     GHSVRLAGGDACVPIWTTNMTRIGGLIPEVDWKRPDDVVERQIDPESGMLATPYCPTT
                     KDEIFVAGTEPTSVCPIHAGSGEPSPFWRDNAEPPQAEGQLQNPAAYPVPNQAPQQKT
                     KPKERGLRKLLRDIFGGN"
     gene            complement(14788..15297)
                     /locus_tag="VNN25_26005"
     CDS             complement(14788..15297)
                     /locus_tag="VNN25_26005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95055.1"
                     /translation="MLASWSEFYFLCGSAAAGLTGLMFIAVTFGSRLITKEKLEYVEA
                     FFSPICYHFIQAFVLCAVALIPIAGPVVLGATIILTTALRIGQLAQTYRLTKAASRES
                     HDIETSDWVLGLILPAAVYVSLIATGVCFLLRNAAAPTLFAISILCLLVIAVRRVWEM
                     LLWIATKVD"
     gene            complement(15371..>16055)
                     /locus_tag="VNN25_26010"
     CDS             complement(15371..>16055)
                     /locus_tag="VNN25_26010"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXH95056.1"
                     /translation="RTGQNWIGAPGTRIADASYVPPPVPEMNAALDALEKFFHQPSHL
                     PALIRLALIHYHFEAIHPFLDGNGRVGRLLITFLFHTEQLLDQPLLYLSAYFEQHRSE
                     YYRRLLDVSRQAAWSEWIDFFLDAVTKQANDAVRRSSQLLALRQDYRARTTSARRSSL
                     LPAIIDRLFESPAITIATVAREFSVTYAAAKNNVEKLINAGVLSAPDAAGRNKVYVAR
                     DIIALLEAS"
BASE COUNT         3093 a         4947 c         5061 g         2954 t
ORIGIN      
        1 cgccttcatg gacgcgagag gcttcaagac gctcgacgac ttccgcagca cccgccgcga
       61 ccgcatcgtc ccccacagcc agatcaaacg ccccgacacc cgcgagtatc acggggggta
      121 tgaggatgag gccgtggagg gatacgcggc agcggaaagg gtgggggtgt agtcctcttt
      181 ggacgatcgg aagaactgaa ccacagagac acagaggaca cagagttgaa acccaacctc
      241 tgtgctctct gtgtctccgt ggtgataagg gttcgctcgg tatatgcgct gctcagcaat
      301 tgcaatcctt ctaatgattg cagcccctgt tctcgctgag ctcccgcccg acgtctacaa
      361 agacatgcag aggaaggcgc cagaggttct gttcattcag gtttcctccg ttcgtgtgca
      421 tcgacgtttc gccaaaccat caggttgcgc tttcttcgat ttcgaggtga ttcgtgaggt
      481 caaggttgat gctcgcattc ttcgcgtggc ccgatcggaa acccgcgttc acgcaggcga
      541 gaccatcgag atcgagtact catcgatcag caggtgttca ggctgggatg gaccgcgatc
      601 gatccaactg ctggagagcg gtgatcgtgt gtacgctttt cttacgagac gcggccgtac
      661 ggcctcgttc gagcccgcgg cgcgaggcgc gacatttgcc ggctcgatgc cgggtgttta
      721 acctaggccg atgcgcccca gcgaaatcat cgagctgtat cgctacaacg actgggcaaa
      781 ccaccgtctc ctgcgcgcgg ccagcatcct ttcaaccgaa gcgcttgccc aggacctcgg
      841 cggcagctac aagagcatcc gggatgtcgt cgcgcacatc gttgccgcgg agtgggcatg
      901 gctcgaacgc tggaacggcc gtagcccgga gtcgatccct gattgggtct caggcagtct
      961 ctccgtcctc ttggaacatc tgtcggacgt cgaggcccag cgtaacgtct ttcttcaaga
     1021 gctttctgac gctgcgctct cgtccgaggt agcgtttcga ttcctgagcg gagcggccga
     1081 tcatcatcca ttacgcgacc tgatgatcca cgtggtcgac cactccagct atcaccgcgg
     1141 ccaactcgcc gccaagttgc ggcaagcagg tgcggttcct ccgccgacgg ggttcattgc
     1201 gttcaagtcg gagttgcggg catcgtgaac actcggtcga tggccctgcc gaggtaacct
     1261 tttcagatgg ccgaaagaca acggcggttt gtcatcctca gagagacggc aattggtccg
     1321 catgagccgg ccgatgtgcg cgtggtcccg gcccacgagg cttgggcagt gacgcccgaa
     1381 agtaccgcga cgacggatgc ctcgctggcg gactacaggg ccggacgcac gattggggtg
     1441 aagtccctgg atgcgcgtat cgccgggcgc aggaaccggc ggaagtagtt tcacacaata
     1501 agaacgtcga cgatttttcg tcctccgccc ttacagcact cgggcgagga cgcccgagtg
     1561 cccgcggtcg agacgaccgc actcctatcg tagcttcctc gccagggcgt cgaacaccgg
     1621 gactgatcgc atcgaatcca gccgggggtc gatgttcagg aagaccatcc atgcggagtg
     1681 atcttcgagc gcttcgtgca gcgcggcgat ggcgcttttc gtgtcgccga gggcggcgtg
     1741 gatgactgct agatcgtagg gggagatgta gcgcgatttc gattccgcgt tgagggcggc
     1801 cagggcttcc tccgcctgtt tgcggtgacc ggcgagggcg tgcgcatggc cgcgcatggc
     1861 catgaggatc gggacgcggt cgacggcgag gccgcgctcg aaggcctgga gcgcttcgtc
     1921 gtggcggccc tgctggctga gggccatgcc gtgccagccg tgggcgggga cgaaatggtc
     1981 gtcgagctcg agcgccttct cgaattgcgc gatcgcaacg tcgtactggc gcgcgaagta
     2041 gtggggccag cccgcgtgga ccgtcacgat cagcgacagc ggatcgaggc tggccgcctt
     2101 cgcgaactgc tcgctcgctg cgtcgtaacg tccgcgcgcg cagagcagca tgccgaaacc
     2161 gtcgtgcacg gcggagtagt tcggattgag tccgatggca cgttgatagt cagcctccgc
     2221 gccgtcccag tcccagtcga accaccactt gaccgcagcc agcgaagccc atgcttcggc
     2281 aagactcgga tcgagctcca cggcccgccg ggccgcggcc tgcgcgcgtg gcatcgcctc
     2341 gcgaggcgga agcggaatgt tggtgccgag ggtgatgtag ctgtcggcca tgccggcgta
     2401 ggcgctggca aacgaaggat cggcatcgat ggcctcgcgg aagaaagcga tgctcttgtg
     2461 caggctctct tcattgcgct tgttccactg gtggcgtccg cgcagataaa gctgataggc
     2521 ggcggtgttt tcggtactgc gtttgcggag acgctttttc tccgcgccgg tcagtttcag
     2581 cctgagcttc tcggcgacct cgcgcgacat ctcgtcctgc agttgcatca ggtcggcgag
     2641 ctcgcggttg tagcgctcgc cccagagctg cgcaccgtca atggcgtcga ccaattcgac
     2701 attgatgacc agccgtccgc cgacatgctg cagccggccc gtgactacgg cacgcacgcg
     2761 caactcgcgt cccactgcct gggcatcgcc ttcacgattc ttgtagcgaa acacggtgct
     2821 gcgggccatg accttcagct tcggcagttg cgacagccgg ttgataatcc cctcggtgat
     2881 gccttcgctg aggtactcgg tctccggatc gttcccgacg ttctgaaacg gcaggacggc
     2941 gattgagtcg atcgacttcg atcgccggcc cggccgccgc tccacctcat tgaggagccc
     3001 actgatcacc gatgaactgc tgccgatcgc gcgcagcgtc aacgcgagat ccctggcgga
     3061 ctggaagcgg tccacgggaa ctttttcgac gcagccttcg atgatccgct ggagctcgag
     3121 cggaaccacg tcgctcaacg attcgcgcgg gatgtcttcc ttgaggatgg cggcgatggt
     3181 cgcagcgccc gaggagcgca gaaacggctt gcgggcagtg accatctcga agagcaggca
     3241 tccgagcgaa aagatgtcgg cgctcggctc gacggactcg ccgcggagtt gttcaggagc
     3301 catgtagccg agggtaccga tcacggtacc ggggccggtc ttgaatcgcg tggtcggctg
     3361 attggtctgg tcgcccgcga cgagaaacgg atcgacctga gccagaccga agtcgaggat
     3421 tttgacgcgg ccctcgtgcg tgagaaagat gttctcaggt ttgagatcgc gatggaccac
     3481 cgatcgagaa tgcgctgccg ccagcccctc ggcgatctcc gcgccgatct caacggactt
     3541 cctccagttc atcggagcgt tgtgtatgcg ctcgcgaagt gtctcccctt ccagcaactc
     3601 ggtgacgacg tagcgcgtcc cctcgtgctg cccaaggtcg aagatggcga ggatgttcgg
     3661 gtgggagagc gaggccacgg cgcgcgcctc acgctcaaat cgcgccatgg catcggtgtc
     3721 gcgcgcggag tcctgggtaa gtattttgat ggccacatcg cggccgagcc gcggatcacg
     3781 tccgcgataa acctccccca tccctccctc accaaggagg gagatgacct cgtacggccc
     3841 gatgcgcgtg ccgggagcaa tcacggaaga gcggtctata tcattcatga ccgccgaaca
     3901 ggagcatcct cgaagacgtt caggtgacga gtagccgttt gcttctgagt tggaacgggt
     3961 accggacctt cgcaggttcg acgttttgac ctaggtcagc cgcttgcgcc aatagttcgg
     4021 cttccggctc gtgtgtgcga gagcgactac ttcgatgtgg tcggtgtatt cgcggtacac
     4081 gagaaagaag gggaaatggc ggagtagaat cctacggact ggtctcggac gtcgaatgcg
     4141 tggtacgact tcgccaatcg acggatgagt agagatcagc gagatcgccg cctggatctc
     4201 tgaccacagt cgatcgccca atccggcgct ctccgcttcg taccagtgga tgttctcccg
     4261 aaactcagcc tcggcgtcgt cattcggccg taccggtttc atcagcgccc gaagatgtct
     4321 ctttcgatgc gagcgcgaac atctttccag tcggaagttg cgagtgtgcc ggcatcaacc
     4381 gccgcacatc gccgctcgat ctcaaccgcc cacgcttcgt ccacatcacg ctgtacttcc
     4441 gggtccaggc tctccagaat cctggtagcc atctcagcgc gatcgttctc cggaaggctc
     4501 aaaacctgat cgagtagggc ggcggtgtct ttcatggcaa tcgaagttta gccgagataa
     4561 gcagagggcg gtccgtttct agagtccagg cccagccctc taccgggaat gcgcgtttgt
     4621 tctcaaggac agatggcatc ccacgtcgac ttcctctctg ctgattctgt ttccgagtac
     4681 ccgactccgc cgcaggcgcc actccccgcc gaggacgacc ttcttgacgc ctactccacc
     4741 gccgttacct cggcggcgtc gcggatcacg ccgtctgtcg tcaacatcga ggtcaggaag
     4801 ccggggccgc gcggcgagga gcgtgggggt gggtcggggt ttctcttcac gccggacggg
     4861 ttcatcctta ccaattgcca cgtcgtcgcc ggggcgtcgt cgatcgacgt gacgctcatc
     4921 gatgggacgc gctgcacggc gaccgtcgtc ggcagcgatc ctcatacgga tctggccgtg
     4981 attcgggtct ccgcgccgaa ccagatcccg gcgcggttgg gcgactcgga tttgattcgt
     5041 cccggacaga tcgccatcgc ggtcggcaat cccctcggct ttcagaccac ggtgacggcg
     5101 ggcgtgatca gtgcgctcgg acggaccatg cgcgcgcaga gcggacggct gatcgatagc
     5161 gttatccaga ccgatgctgc gctcaatccg ggcaattcag gcggtccgct cgtcaattcc
     5221 cgaggagagg tcatcggggt gaatacggcg gtgatccggc cggcgcaggg aatctgtttc
     5281 gcgatcggcg tcaacatggc caagttcgtg gcggcacggc tgatccgtga cggccacatc
     5341 acgcgcagct atatcggcgt cgccggtcag ggcgtgtcgg ttcatcgccg cctcgttcgc
     5401 ttctacaacc tggctgtgga gagcggcgtg ctcgtacaca gcgtggagaa gaagagtccc
     5461 gcggaacgat cgggagtgcg cgagggagac atcatcgtcg ggtatggcga tactcccgtc
     5521 tcctcggtcg atcacctcca ccgcatcctc agcgaggagc agatcgggag ggagtccacg
     5581 ctcactttga ttcgatcgac ggagaaggtg atcgtcagca ttacgccggt tcccgctgcg
     5641 gcgcagtgaa actcggaacc gccctactgc gaatgcacgg gcggcgtgcc atcgcgcagg
     5701 aagtccgaga ggatctggat gcggtggggg atgaactgct tttcgatgat cggatcgatg
     5761 ccgtagcggg ttgatggtcc cgcctcgccg tcggccagga tgtcttcgct gatcatctga
     5821 tcgccctgga agccgatgac gagcttgacg ttcccgccga aggggacggc gccgttgaat
     5881 ccgtggcaac cggcgcatga ctgttcctcg gcgcgcacga tgacgttgtt cggcgtgaga
     5941 ctactgccgg cgagcgtgag ctccgattgg atgtttgccc ggaaggcaac gccgtcggac
     6001 gagctctgcc cggagttgaa tgcgatctca aagaggacga cgcccggagt gcccggcgta
     6061 tcgctctcgg ccatcagata gtccttcgga aggcgcatgc tcatcaggtt cagatcttcg
     6121 atggcaagcg tcggaacgtg tttcaggaat tcggtgcgca gcgcgcgggc ctgcgccgag
     6181 gtgttggtgc catcgaagag gagtccgaag ggctggttct cgaggacgtc cgggatgaag
     6241 cggagcgtgc aaccggcgga gccgcactgc ttcgccaagc gaaactgata gaagcggatg
     6301 ctgacattcc cgccgtccgc tgactgctgc aacgtgcgga tcccgccggg atcggcaaag
     6361 ttcgcggcat cgacgacggg agcgaacccg tcgagaccct gaaagaagaa cctctcgagc
     6421 cgcgcgcggc gctcgtccat cgagtcgaca gcggtcaggt ccgcccagaa cttcgcgatc
     6481 ggacggcagg ccgcgagacc gagggccgga ttcggattcg gcaggacggc ttcgaagatg
     6541 agatggcggc gcaccagcgt gctcgcgctg gtttcactgt cgcggtgcgc gaagaccatg
     6601 cggtactgac cgcagttcga tccatccggc ggcgcctgat cgaaacgatt catcagtccg
     6661 agcgtgaaga actggccggc gaggttctgt acggcaacca actttgcttc cggcgtcggg
     6721 catcggcgtg ggaatccgtt gaacgatgga acgccgttcg tcagcgtatc gtcgcaatga
     6781 ggaccatccg gatcgatggc gcccgaccgc ggggtctgcg tgtcgaacat ccgtcggatc
     6841 aactgatccg cggtgagtcc gttcacgccg ctacgctcga tcaattgcgt gagcactcgt
     6901 tcgagggtga atgacgcgac gatcgatttg tcggtgatct cgaaggaacg cgtggcgttc
     6961 agaatcacgg gggtttcggg cggatgcgaa ggccgatttc tggccgcagg gagcaggatg
     7021 acgatcgcaa tcgcgatcgc cagagtttcg atcgtgctgc ggcgcatggt gcaaatgcta
     7081 gtggatattg gaactggccg ctaacccaac ccatcgtaga aacccccttc ccatggagcg
     7141 ccacgcgcgc gatgtctacg ttcgccaaag caaagacaac cacaaagaca cagaggacac
     7201 gaagagtctc tgtgaccttt gtgcctttgt ggtgaccggt tctagaaatt cagtactccg
     7261 cgaacgcttc gtccggatag cagtagagcc agcgctcgcc cggctcggcg gacgcgatta
     7321 ccgggtggct ggtcgcgtgg gcgtgtttgc tggcgtgccg gttgggtgag tcgtcgcagc
     7381 agcgggtggc gccgcactcc tggcaggtgc gcaggtggac ccagcgggaa ccgatcttca
     7441 cgcaatcctc gcactcgcgt ttcttcgcat gtttgacggc cgtgatcgca ccgatatgtg
     7501 tacagctctc atccggcatg ggaactcctt ttcactgatg aagcacctga tggacgaagg
     7561 cgatggcaat ggagccctcg ccgacggcag aggctacgcg cttgatgttg ccgcctcgca
     7621 catccccgac ggcgaacacg ccgggaagac tcgtctcgag aaggtacggc ggccgggtga
     7681 gcggccatcc cgcggtcacg aggtcgtccg gcgtaagttc cggtcccgtc ttgatgaacc
     7741 ctttgtcatc gagcgcgaca cactgctcga gccattgggt gttgggcgcc gctccggtca
     7801 tcatgaatac gtggcggatg tcgtaggtct cgacagcggc gttccgattg tcgcgccagc
     7861 ggacccgttc gagatgacgc tccccttcaa ggccgacgat ctccgcacgc gtgtgaaggg
     7921 tgattccggg gttgtcttcg atgcggcgaa tcaggtagcg cgacatgctg tccgccagca
     7981 cctccgcgcg gaccatcatg tgcacgtgct tcacgctctg cgcgagaaac accgcggcct
     8041 gccccgctga attgccaccg ccgacaacga tgacttcctc gtccacgcag agttgcgcct
     8101 ccatcggcgt cgcgccgtag taaagccctg cgccttcaaa ctgcatggcg ttctcgaatg
     8161 gaggcctgcg atactcggcg ccagtggcga tgatcaaggc acgagtcagg acgcgctgcc
     8221 catcgtcaat ctgcaccgcg tacggcttcc ggttgcaagt caacttggca gcacccttcg
     8281 cgatcatgat ttgcgcgccg aacttctgag cttgcgcgta cgcacgcgcc gtgagctcca
     8341 gccccgaaat acccgtcgga aagccgaggt agttctcgat ccgggagctc gatccggcct
     8401 gtccgcccgg cgcgttcaac tcgaacacga ggacatcaag cccttcggac gctccgtaaa
     8461 cggccgccga aagcccagcc ggccccgccc cgacgatgac gacgtcgcgt agctgcgccg
     8521 tatcgatcgc cgggttgaag ccgaggcaat cggcgatccg ctgattggaa gggttgcgca
     8581 gcactgcgtc gccgcggcag atcaacactg gcacatcgcg cgcgctcacc tgaaaccgat
     8641 cgagcagatc ctgcgcctcg gcgtcgtgat cgaggtcgac gtaggcgtac ggatgaccgt
     8701 tgcgggtcag aaactccttc acccgcagcg tgccggcaca atgcgccgaa ccgatcagca
     8761 cgacatcgcc gagaccgcgc gcgatcagct cgacccggcg gaggatgaac gcccgcatca
     8821 gaatctcgct caactcggcg tcggtctgga tgagtgcgag gagctgctca cgatcgagct
     8881 cgatcacttc gcccggctcg ctgacgcgga tgcgcgccaa cgaccggcgg cccgtgatca
     8941 tggtgccttc acccgagaac tgcccgcgcc ggtgtgtgac gatgagtgtt tcggtaccgc
     9001 gggatggccg caaaacctcg atctcgcctt cgacgacgac gaagaacgga acgaccttct
     9061 ctgctacatc gacgagcaca tctccgcgcg caacggcgcg gcgccgccca tgcgccgcga
     9121 tccgcgccac ttgcgcgtcc gtcaacgtcg ggaacaggtg ctcctgaacc gatgacgcgg
     9181 acggctccag cgccacggca gggggatcgg acgcgttcac ggcgtggccc tccagcatct
     9241 cgtcaagtat ggcatcggct gattatccgc gagaggaatg catctaacga gcctgaggct
     9301 ggggccggta gcgttcgatc gagtcgtgaa ggccattacg cggaaccgcg gttcgttcgt
     9361 atcagccggc aaggagaact acgcatgaac aaaactcgat ggtcaagcct ggcagtggca
     9421 gtcagcctgt tgttcgcgtt tacggcgatc gcacagcagg gcggcggcca gcagggcgga
     9481 cccaaccaga ccttcacgca gcgctatggc cagcaactca acctgaccga cgatcagaaa
     9541 aagcagatcg acgagatgga cgcgaagttc cagaagaaca acgcagagtt cctggagtcg
     9601 tatcagaaga cgatggcgga ataccgcgag gcacgtcagg caaacgacac cgccaagctc
     9661 gacgctcttc gccctaaagt cgattcggcc cgtgcggaaa tgtcgaaact gcgcggcggc
     9721 cacgaagaga agatcgcggc cacgttcaac gatgcgcaga aagcgcagtg gcagaagatc
     9781 aaggaagagc gcgaagctcg catgaaggag cgccagcagc atcagtagat cagcatcgcc
     9841 tccacccaat gcgtggcctc tcggggccac gcatcctcgc cccattgacg atttcgaaga
     9901 cggctcgcaa gatggatcgt gatgtactgc gtattcatcc acgggccagc cgcctcaggc
     9961 aaactcacgg tggcgaatgg cttgaaggcg cgttccggtc tgcccgtctt tcataatcac
    10021 ctggccgtcg acgccgcact gtctctcttc gagtttggct ctcccggctt tgtccggctt
    10081 cgcgaagccg tctggctggc cgcgttccgg gaagctgcgg agagaggacg gtcgttcatt
    10141 ttcaccttct cgcccgaggc gtctgttccg ccggctttca tcgacacggc tgtcgatgtc
    10201 atcgagggcg ccgggggtca ggttctcttc gtctcgctca catgcggcga ggcggtcatc
    10261 gaagagcgta ttgaggccgc cagccgttca gcattccgaa agctgacgtc tctggcggag
    10321 tatcggcaac ttcgcgaacg tggcgccttc gcctttccgc cccttccggc agacatcacg
    10381 atcgcaaccg acgagctttc gccggaggat gcggcgggac tgatccagga gctgatcggg
    10441 aatcggggca tccactgagc cttcggaggt cagtgattgg cctcacgcga aggcgcgaag
    10501 acgcgaagga acgtcttggt tttcaaacgg cccgtttcga ggggaacgtc ttcaatgtcg
    10561 aagcagccgc gttgaatgtc caggcagacg cattctgttc aagggtccaa caccacgtcg
    10621 agcacggggc gatctggcct cgccggctcc cagctccgtt gtcagttcgt cctgaagttg
    10681 accgttagaa ggaagacgac gtcgactggt acgccccgaa ggcggcccgg ttcgaaggtc
    10741 cattgggaga tcgccatgag ggcggcgccg ttcatctccg gatagggcga ctgccggacg
    10801 agttgcactg cgcggacgca accgtcgcga gaaatgatgc agcgaagaac gacactaacc
    10861 atcgcacttc ccaccattcg atgccgcgcg gattggggga actccggctc cttacggcgc
    10921 ttgacgattg gtgccgtgat ttcgccgccg acatcgcgag gcgcgccgtt tgatgcgtca
    10981 agaggcgctt catcgagaca aggcggctgt ttgaatccgt gaacgaggag gatgctgctg
    11041 gcctccatcg gcacgtcgag cgacgttggt gtcaccagag aaaggcgcat caaggtggtg
    11101 accgctttgt cgcaggcagt gtccatatcg atccgcttga ccgtgaccgc ctgcacgcgg
    11161 ccggcggaat cggccgtcgc tccggccagg ccaagccacg acccgttgca gtgagtctct
    11221 gcgaccaccg cagatgcgac gccaggaggc aagaggtcgg gcagaaagaa ggccggctcc
    11281 gccacatccg tcgacggaat cacgcgagcg gcggaccgcc gctcgggcat ccggcagtcg
    11341 taactggtga cgccacaatg attcttgcgg acaaggaact ccttctccgt gaagtgctga
    11401 aacaaagcct cgtccgtggc caggagcgga tcagcctcgt tggtctgcag ccattccagc
    11461 cagcgcggat catccttctt ttccccaccg agcatccgtc gcagtagttc acggccgaac
    11521 gcttcgcgcg tgctggcctc ctccgtcgga gtgctgagaa tggttcgtac gcgcggagcg
    11581 atcatggcgg gatcaggcac gtaggcgtgc accaagtacc agaccgacgc tgttcggatc
    11641 tcttcggacg gcatgttgag cgatgccgcg agcacatcgg cttgcatcgc cagatgagcg
    11701 tccttgagcg ctgccagaag tgcacgccag ccggcggcgt cgcgcatgcc gaggagtcgt
    11761 gaaccggcgg cgatcgccgc ggaaggacgg tgccaaagcg cctgcgtgaa gaagcttgcg
    11821 tcgggaatga agccgccggc gcggaggagc gacacataga tatcgaacgc atcggtccgt
    11881 cgtgccaccg ctcgggcgat cacatcgtcg atgccgggag caaacttgcg cgtcgcggct
    11941 cttgcgcggt cgacatcggc cggatccccg agaatgacga gcgcgcggac ttcctcgcgg
    12001 gccgccgacg aatcgctttc gaccatcagc gcatcgtgga tctgcggaag cacacccgtc
    12061 agcccgcgga caagcgcaac gcgtgacgcc gccgcgcgcg tgacggcatc cgtcgcggca
    12121 agagctggcg ccacgcgtac ggctaccgca gccgcgtctt tctcgttcac gagaagcgcg
    12181 acggaagccg ccgtttcgcc ggccgtcgcc gtgaccgcca caaggatcgg cagcaagagc
    12241 gccagcaatg ttttcgtcat tggccggaga gtagcaacaa atgagggatg agacgcgcct
    12301 tccccttttg ttggtaccct cacatcgatg gctaaccgct cccggatcaa agatttcctc
    12361 tggcgtcacg cggctgggat cgcctcgatc gtcatggtct gtttcgtggc gttcctggcc
    12421 ttcgtcggat actcgtacgt cgtcctgacc aagaaattcg attccagccg gcgctgggat
    12481 ctgccgagcc gcatctactc tgatgccacg ccgatcgtgc ccgggatgac cattccgcgc
    12541 tcgctgctgg agccgaagct gaaccatgtc ggctatcacg agtcggtatc gcgcgtgacg
    12601 aatccgggcg agtaccgcta cgcgggaaac acgctcgaga tctacctgca gaacttcgag
    12661 tatcccgaca tcgagttccg cgctctccct gtcgtcattc agatggacgg gggtaccgtc
    12721 cgggccattc accgcctcga cgacggcgtg gccctccgcg gcatccgcat cgagccggag
    12781 ctgatcacct cgatctacaa caatgagatg gaggaccggt tgcccatcgc cctgagcgcg
    12841 gtgccgaaga cggtctcgaa tgccatcatc gccgtcgagg acaagggctt ctacagccac
    12901 agaggtctct cgatcacagg catcctcggc gctctgcgca cggacgtcaa gaatcgcaac
    12961 atcacgcacg gcggctcgac gctgacgcag cagttgatca agaacctcta tctcaatccc
    13021 gagcgtactt ggcgccgaaa agcgatcgaa gcgctctacg cggtgctgct cgaggcgcgc
    13081 tacagcaagc aggagatcct cgagggctat ttgaacgaga tctacctcgg acagaacgga
    13141 gcggtgcaga tcatcggcgt ggagcaggct tcgcaggtct acttcggcaa acacgtcacc
    13201 tatctcacac tgccggaagc ggcgacgttg gccgggatga tccattcgcc gaacgtcgag
    13261 aatccgctga agtatcccga gcgcgccaaa ccgcgccgcg atctggtcct gagcctcatg
    13321 cgcgatcaga atctgatcac gccagccgaa tacgacgcag ccgtggcgtc gccggtgacg
    13381 acggcgcgct tcccgaaaac ctcacgcagc gcgcccttct tcgtcgatct cgtgctcaag
    13441 cagttgaagg agacgcatcc ggagacacag ctgcgcaccg aggggctgcg cattttcacg
    13501 acactcgaca cgatcatgca acggtccgcg gagcagtcgc tcgacgaggg catcgcctcg
    13561 ctcaacaaac actactcgta cctgcgcaac tcgccgacgc cgctcgaagg agtcatgctc
    13621 acgatccagc cgggaacggg ctacgtgaaa gcgctcgtcg gcggccgcaa ctactcgaaa
    13681 acgcagttca atcgggcaat ccaggcacgg cgccagcccg gatcgctctt caagccgttc
    13741 gtttacgcga cggcgatgga tcctgctcgc gggcagcagg cattcacggc cgcaacggtt
    13801 ctcgacgatt cgccgatcag cgtccgcacc ggcaacgcgg tctggacacc gcagaactac
    13861 gacctcaagt accacggtca cgtgacggtg cgtgaagcgc tggcgcactc gtacaacatc
    13921 ccggcggtac gcgcggcgat gaacgccggc gttccgaacg tcatcaagac cgcctcgaca
    13981 atcggcatcg agagccctct cgcgccgtat ccgtcggtcg cactcggctc gttcgaagtc
    14041 acgcccctcg aaatcgccta cgcctactcc gctttcgcga acctgggcgt caaggccgag
    14101 ccaatctcga ttctcgcggt ctccacgagc gaaggcgtcc tgctcgagtc gcgcgaggtg
    14161 aagatgaagc gtgtcgcggg atcgggcgtg tcgtacgtga tgaaccagat cctgaaagac
    14221 gtgttcgttt acggcacagg cagcaaggcg cggcaaatgg gtttcacgcg gccgttcgcg
    14281 ggcaagtcgg gaacgaccag cagctaccgc gacgcctggt tcatcggcta ctcgccgcgc
    14341 attctgtcgc tggtgtggct gggtttcgac gatggccaca gcgttcgcct cgccggtggc
    14401 gacgcatgcg ttccgatctg gacgacaaac atgacgcgca tcggcgggct gattcccgaa
    14461 gtcgactgga aacgccccga cgatgtggtg gagcgccaga tcgatccgga gtcggggatg
    14521 ctggccacgc cgtattgccc gacgacgaag gatgagatct tcgtcgccgg aacggagccg
    14581 acgtcggtct gcccgatcca cgccggcagc ggcgagccaa gcccgttctg gcgcgacaac
    14641 gccgagccgc cgcaggccga ggggcagttg cagaatccgg cggcgtatcc cgttccgaat
    14701 caggcgccgc agcaaaaaac gaaaccgaag gagcgcggcc tccgcaaact gctgcgggat
    14761 attttcggcg ggaactagcg gctccgatca atcaactttc gtagcgatcc acaagagcat
    14821 ttcccaaact cttcgcacgg caatcaccag taggcacaat atcgagatcg caaagagggt
    14881 gggggcggcc gcgtttcgca gcaggaagca aacgcctgtc gcaatcaacg acacgtaaac
    14941 ggcggccggc agaatcagtc cgaggaccca gtcggatgtt tcgatgtcgt gcgactcccg
    15001 tgaagccgcc ttagtcaggc ggtaggtttg cgccagttgc cctatccgaa gagcagtcgt
    15061 caagatgatg gtcgcaccga ggactaccgg gccggcgatc gggatcagcg cgacagcaca
    15121 aaggacaaac gcctggatga agtggtagca gatcggcgaa aaaaaggcct cgacgtactc
    15181 gagcttctcc ttcgtgatca accgcgaccc aaaggtgacc gcgatgaaca tgaggcctgt
    15241 caggcccgcg gcagccgatc cgcacaggaa atagaattcg ctccagctgg ccagcacctt
    15301 gtaacgatat ccgctctcgg ttttcggaaa aaggcggtgc gcgcttcaag tactcgtacc
    15361 gcgggcaagt tcaggaagct tcgagcaagg cgatgatgtc gcgcgcgacg tagaccttgt
    15421 tccgtccggc cgcgtcaggt gcgctgagaa ctccggcgtt gataagtttc tccacgttgt
    15481 tcttggccgc cgcgtacgtg acagagaact cccgcgcaac ggtcgcgatc gtaatcgccg
    15541 gcgattcgaa aagacggtcg atgatcgcag ggagaagcga tgatcttcgc gcagaagtcg
    15601 ttcgagcccg atagtcttgc cgcaacgcca gaagctggct cgaacggcgc accgcgtcat
    15661 ttgcctgctt cgttacggcg tcaaggaaga agtcgatcca ctcgctccat gccgcctggc
    15721 ggctgacgtc gaggagacgt cgatagtatt cgcttcgatg ctgctcaaag tacgcgctga
    15781 gatagagcaa gggctgatcg agcaactgct cggtgtgaaa gagaaacgtg atgagtagcc
    15841 gtccgacacg gccgttcccg tcgaggaagg gatgaatcgc ctcaaagtgg taatggatca
    15901 acgccaaccg aatgagcgcc ggaagatgcg aaggctgatg aaaaaacttc tccagggcgt
    15961 cgagcgcggc gttcatttca gggactggag gcgggacgta agaagcgtct gcgatacgtg
    16021 ttcccggcgc gccgatccag ttttgcccgg ttcgg
//
LOCUS       DATLVW010000881         6142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_407012, whole genome shotgun sequence.
ACCESSION   DATLVW010000881 DATLVW010000000
VERSION     DATLVW010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407012"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..2150
                     /locus_tag="VNN25_26015"
     CDS             297..2150
                     /locus_tag="VNN25_26015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95057.1"
                     /translation="MFRRLSLAGILLILAMPSFAATEGGAIPVPLPLFPANNWWNTDV
                     SNAPLDANSANFITFIGGASRGLHPDFGGDAGGGDVYGFPFIIVDGSQAKKTVTFTDF
                     GDQSDGVNHVTGQSFPFYPIPDEAITMNGWVEGGQPGNIDQRADQDRHVLIVDQTNNT
                     LYELYNVWYDGTNWEAASGAFFDMKTNNRRPDTWTSADAAGLAILPGLVRYDEVFGPD
                     EIRHAFRVTVQSTNGYVFPASHEAGSASGALPMGARLRLKADKDISSFPASVQKIFRA
                     FKKYGLIVADNGSNMYISGTYDVRWDNDVLNPAFGALKASDFEVVQRGWKPPISLVIT
                     MPSSVGTGNANDATVTAYDATYNVAAGYTGTIHFTSTDGAATLPLNYTFTGGDAGVHT
                     FTGGVTMRTNGSQIITATDVADATINGSKGVTVSVFPSTPAGLTATAATTTHVAVSWS
                     AASGATQYELVRKSSSSGYTTLITTSSTNYSDTSAVAGASYVYKVRAIDAAAHQSAFS
                     TPDAATTIFFTDDPLIAATTPTKAVHVTELRQAVNAMRATAGLGAASFTDASLAGVAV
                     KATHIQELRTALNQARTTLGISAITFTDPALTAGTSILRAAHIQELRGGVK"
     gene            2268..4823
                     /locus_tag="VNN25_26020"
     CDS             2268..4823
                     /locus_tag="VNN25_26020"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HXH95058.1"
                     /translation="MNKRSLLIFFAAIGVVTASHAANWPEHPVLHAVRVAKGPVIDGD
                     LSDAAWQNAPEFTEFTQHDPDDGKPPTMATSIRIVYDDQAIYFGAKMTDPQSPSRVIF
                     RRDNFSDQSDFLSINIDSQHDHLSGNAFTVTPSNVQADTVLYNDIGEDPSWDGVWDSA
                     VKIVPDGWVAEVRVPFSQLRFPDKPVHVWGLNITRRTVRNNEWVRIVNTKKGETGFVS
                     HFADIVGLEGIHRGRPLELVPYVVTRTDLLSHKDPNNPLLQNHDYKGDGGLDVKYGLT
                     SSLTLTGTVNPDFGQVEVDPAVVNLSQFETFYPEKRPFFTEGVNIFRFGDTPAPSHFN
                     FFFPPSLFYTRRIGRAPQVSPDADFFVSPSDTTILGAAKLTGKLPGGWAIGVLDALTA
                     AEHARFVSGGVSGRQTVEPLTNYFVSRGTKEIGDGNRLGFMLTSVNRSLPDDLSSLRS
                     DALTAGVDGYASFLKKSWILEGSAVGSKVTGSEDAIALAQTSSSRYYQRPDAENIQFD
                     PTRTSLTGWGGRIMLSKATGFWRPNVQVQAFSPGFETNDTGFMQRTDIISSHALMQYV
                     NDNPNEHFRQRGAYMGVWQNRNFDGNTIEQGAFIDSQATLLNYWFVRGSLFLNTEALS
                     DRLTRGGPLVRTPASWSSDQNISSDTRKRFTVGWNGHIDHSQDGSYNHSMSVDLSVLP
                     MPNLHLTVSPAVTRSHDHTAYVKAFSDPTALATYGTRYVFADLEQRSFELGTRADWTV
                     SSHLSFQLYLQPFVAAGDYHDFHSLAAARTRDYDAFTGDPGNPDFNFRSVRGSAVVRW
                     EFRPGSALYVVWNENRADVAPIGDFRFGRDFRAIRTEPSHDVFLVKVSYWLPL"
     gene            complement(5291..5800)
                     /locus_tag="VNN25_26025"
     CDS             complement(5291..5800)
                     /locus_tag="VNN25_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95059.1"
                     /translation="MWTARRIIKELADSDRRRRILVAFWRFGDDTAKMLAVAQLAKAL
                     HFREESIRKLPVEKKAEMFASRATAPEFEQALDTALMHYHTHEQSALLAAFLDQWKIP
                     HVNGSIEGDDYAAPSEEQVRDAVKAVDGFPPRDVVIYLASAGLLMGDDWRAATWPVVD
                     ELAPKIGDQ"
     gene            complement(5899..>6142)
                     /locus_tag="VNN25_26030"
     CDS             complement(5899..>6142)
                     /locus_tag="VNN25_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HXH95060.1"
                     /translation="IELRDGPTRPARVERVGERNFEITITEGRNRQVRRMVEALDAEV
                     LKLVRVAIGPIRIDDLPVGKMRELTKDEVRVLGGGA"
BASE COUNT         1143 a         1940 c         1877 g         1182 t
ORIGIN      
        1 ccactcccgc aggctggcaa ttgggatctg accgtgtggt cgtctcccat taccagcgtc
       61 gccaagcttc acgatgtggc ctttcaggag cgcgacgcgc tgcgcgagat caggatcacg
      121 ctccccgagg ctacggtgac gcggctggct cgttgattgg tccgcagatg tcgcagatgg
      181 gacgcagata aaactgtctt catctgtgtc tatctgcgtc atctgcggat cagcttgtct
      241 gtcccgttgt gagaaccctc acaacctgcc gcacagcgca gcggacagaa tgacgggtgt
      301 ttcgccgtct ctctcttgca ggcatcctct tgatcctggc gatgccgtcg ttcgccgcga
      361 cggaaggcgg cgcgattccc gttccgctgc cgctgtttcc ggccaacaac tggtggaaca
      421 cggacgtcag caacgcgccg ctcgacgcca actccgcgaa cttcatcacg ttcatcggcg
      481 gggccagccg aggactgcat ccggatttcg gaggggatgc cggtggcggc gacgtctacg
      541 gcttcccgtt catcatcgtc gacggatcgc aggcgaagaa gacggttacg ttcaccgact
      601 tcggcgatca gagcgacggc gtcaatcacg tcaccggcca gtcgtttccc ttctatccga
      661 ttcccgacga ggccatcacg atgaacgggt gggtggaggg tgggcagccc ggcaacatcg
      721 atcagcgggc ggatcaggac cgccacgtgc tcatcgtcga tcaaacaaac aacacgctct
      781 acgagcttta caacgtctgg tacgacggta cgaattggga ggctgcatcg ggcgcgttct
      841 tcgacatgaa gacgaacaac cgccgccctg acacctggac ctcggctgac gcagccggcc
      901 tggcaattct gcctggtctc gtgcggtacg acgaggtctt cggcccagac gagatccgtc
      961 acgctttccg cgtcacggtg caatcgacga acgggtatgt ctttcccgcg tcacatgagg
     1021 cagggtcggc gtcgggcgcg ctgccgatgg gcgcgcgcct gcggctcaag gcggacaagg
     1081 acatctcgtc gttcccggcc agcgtgcaga agatctttcg cgcgttcaag aagtacgggc
     1141 tcatcgtcgc cgataacggt tcgaacatgt acatcagcgg aacctacgac gtccgctggg
     1201 acaacgatgt cctcaatcct gcattcggcg cgttgaaggc gagcgatttc gaggtcgtgc
     1261 agcgcggttg gaagccgccg atctcgctcg tgatcacgat gccgtcgtcg gtggggacgg
     1321 gcaacgcgaa cgatgcaacg gttacggcgt acgacgcgac ctacaacgtc gctgcgggat
     1381 acacgggaac gattcatttc acatcgacgg atggcgccgc cacgttgccg ctgaactaca
     1441 cgttcactgg cggtgacgcc ggagtgcaca cgttcaccgg cggcgtcacg atgcgaacga
     1501 acggcagcca gatcatcacc gcgacggacg ttgccgacgc aacgatcaac ggctcgaagg
     1561 gcgtgaccgt gtcggtgttt ccatcgacgc cggccggact tacggcaact gccgcgacga
     1621 cgacgcatgt cgcagtgtcg tggagtgcgg cgtcgggcgc gacgcagtac gagctggtac
     1681 gcaagtcatc atcgagcggc tacacgacgc tgatcacgac ctcgtccacg aactactccg
     1741 acacctcggc ggtcgcaggt gcaagttacg tctacaaagt gcgggcgatc gatgccgcgg
     1801 cgcaccaatc cgcgttcagc acccccgacg ccgccacgac gatcttcttc accgacgatc
     1861 cgctgatcgc cgcgacgact ccaaccaagg ccgtacacgt gaccgagctc cgccaggccg
     1921 tcaacgccat gcgcgccacc gccggcctcg gcgcggcttc attcaccgac gcatcgctcg
     1981 ccggcgtcgc cgtgaaggcg acgcatatcc aggagctgcg caccgccctc aatcaagccc
     2041 gcacaaccct cggcatcagc gcaatcacct tcaccgatcc ggcccttacg gccggaacgt
     2101 cgattctgag agccgcgcat attcaggaac tgcgaggcgg agtgaagtag tcaccaaaac
     2161 catcaccgca gagacacaga gatcacagag actttttatc tctgtgacct ctgtgtctct
     2221 gtggttcgtt ttttcttccg ctccgatccc gtattcttcg cacgcacatg aataagcgct
     2281 ctcttctcat ttttttcgcc gccatcggcg tcgtcacggc gtcacacgcg gccaactggc
     2341 cggagcatcc ggtgcttcat gccgtccgcg tggccaaagg tccggtcatc gacggcgatc
     2401 tctccgatgc tgcgtggcag aacgcgccgg agttcaccga gttcacgcaa cacgatccgg
     2461 acgacggcaa gccgccgacg atggcgacga gcatccgcat tgtttacgac gatcaggcca
     2521 tctacttcgg cgcgaagatg accgatccgc agtcgccctc gcgcgtcatc ttccgccgcg
     2581 acaacttttc cgatcagagc gactttctct ccatcaacat cgactcccag cacgaccacc
     2641 tttcgggcaa cgcattcacc gtcactcctt ccaatgtcca ggccgacacc gttctctaca
     2701 acgacatcgg cgaagacccc agctgggacg gtgtctggga ttcggcggtg aagatcgtgc
     2761 cggacggttg ggtggccgag gtgagggtgc cgttctcgca gttacggttt ccggacaagc
     2821 cggtccacgt gtgggggctc aacatcacgc ggcggaccgt gcgcaacaac gaatgggtgc
     2881 gcatcgtcaa cacgaagaaa ggggagacag gcttcgtctc gcacttcgcg gacatcgtgg
     2941 gattggaagg aatccatcgc ggacgtccgc tcgagctcgt gccgtacgtg gtcacgcgaa
     3001 cggacctcct gtcgcacaag gatccgaata atccgctgct gcagaatcat gattacaagg
     3061 gcgacggcgg tctcgacgtg aagtacgggc tgacgtcgag cctcacgctg accggcacgg
     3121 tcaatccaga cttcggccag gtggaagtcg atccggcggt cgtcaatctc agccagttcg
     3181 agacgttcta tccggagaag aggcccttct tcacggaagg ggtgaacatc ttccgtttcg
     3241 gggatacgcc tgcgccgagc cacttcaact tcttctttcc gccgagtctt ttctacacgc
     3301 ggcgcatcgg ccgcgcgccg caggtatcgc cggacgcgga tttctttgtc tcgccatccg
     3361 atacgacgat cctcggcgcc gcgaaactca caggcaagtt gccgggcggt tgggcaatcg
     3421 gcgtgctcga cgcgctcacg gcggcggagc acgcgcgctt cgtgagcggc ggcgtgtccg
     3481 gacggcagac cgtcgaaccg ttgacgaact atttcgtctc tcgcggcacc aaggagatcg
     3541 gcgacggcaa ccgcctcggc ttcatgctca cctcggtcaa ccgcagtctt cccgatgacc
     3601 tctcctcgct gcgcagcgat gcgctgaccg cgggcgtcga cggatacgcg agcttcctga
     3661 agaaatcgtg gatcctcgaa ggctcggcgg tgggatcgaa agtaaccggc agcgaggatg
     3721 cgatcgcgct ggcgcagact tcctcgtcgc gctactacca gcggcccgac gcggagaaca
     3781 tccagttcga tccgacgcga acctcgctga ccggctgggg cggccggata atgctctcca
     3841 aggctacagg tttctggcgt cccaacgtgc aggtgcaggc gttctcaccc ggcttcgaga
     3901 cgaacgacac cggcttcatg cagcgcaccg acatcatctc gtcgcacgcg ctgatgcagt
     3961 acgtgaatga caatccgaac gaacacttcc ggcagcgggg cgcctacatg ggcgtctggc
     4021 agaaccggaa tttcgatggc aacacgatcg agcagggagc attcatcgac tcgcaggcca
     4081 cgttgctcaa ctactggttt gtgcgcggct ctctttttct caatacggaa gcgctgtccg
     4141 atcgactgac tcgcggtggt ccgctcgtgc gtacgccggc gagctggagc agtgaccaaa
     4201 acatctccag cgatacgcgc aagcgcttta cggtgggatg gaacgggcac atagaccatt
     4261 cgcaggacgg ttcgtacaac cactccatga gcgtcgatct gtctgtgctg ccgatgccga
     4321 acctgcattt gaccgtcagc cctgccgtga cacgttcgca cgaccacacc gcgtatgtga
     4381 aggcgttcag cgacccgaca gcgttggcca cgtacggaac gcggtacgtg ttcgcggacc
     4441 tggagcagcg ttccttcgag ctcggcacgc gggccgactg gacggtcagt tcgcatcttt
     4501 ccttccagct ctatctgcag ccgttcgttg ccgcgggcga ctatcacgat ttccactctc
     4561 tggcggcggc gaggacccgc gattacgacg ccttcacggg cgatcccggc aatcctgact
     4621 tcaacttccg cagcgtccgc ggcagcgcgg tggttcgctg ggagttccgg ccaggctcgg
     4681 cgttgtacgt ggtctggaac gagaaccgcg ccgacgtcgc gccgatcggc gacttccgct
     4741 tcggccgcga ttttcgcgcc atccgaacgg aaccgtcaca tgacgtgttt cttgtgaagg
     4801 tttcgtactg gttgccgttg tgacgggggt cggttttcgg gggtcgggtg tcggagcgat
     4861 gcagatgcgg agtcggaagg ttggcgccgg agtgaggtgg tactacctga cttcacgttc
     4921 ttcgcggctt agttagcggc gcggaacttg gcttcgccga tgaggattta cgtaggtcgg
     4981 actctccagt ccgacatcac gtgcagtcgg actggagagt ccgacctacg taaaacgcat
     5041 cctgatgggc tgcatcagga attggcggaa gttcgtgcat tagcggcgcg gaacttggct
     5101 tcgccgatga ggatttacgt aggtcggact ctctagtccg acatcacgtg cagtcggact
     5161 ggagagtccg acctacgtaa aacgcatcct ggtggactac atccggaacc ggcggaagtt
     5221 cgtacattcg cgtgatatcg gggttcacgc gaagacgcga agacgcgaag gaagactatt
     5281 tggccacgaa tcactgatcg ccgatcttcg gcgccagctc atcgacgacc ggccacgtcg
     5341 cggcgcgcca atcgtcgccc attaacagac ccgccgaggc gaggtagatc acgacatcgc
     5401 gcggcgggaa tccgtcgacc gctttcacgg cgtcgcgcac ctgctcctcc gatggcgcgg
     5461 cgtagtcgtc gccctcgatc gagccgttca cgtgggggat cttccactgg tcgaggaacg
     5521 ctgccagcag cgcgctctgc tcgtgcgtgt ggtagtgcat cagcgccgtg tcgagcgcct
     5581 gttcgaactc cggcgccgtg gcgcgcgagg caaacatctc cgccttcttt tcgacgggaa
     5641 gtttgcggat ggactcctcg cgaaagtgca gggccttggc gagttgggcg acggcgagca
     5701 tcttcgcggt gtcgtctccg aagcgccaga aggcgacgag aatgcggcgg cggcggtcgg
     5761 agtcggcgag ctctttgatg attcggcgtg cggtccacat gtgatcttga tcgtataacg
     5821 ggggtgggga gtggggggtg gggagtggga gcgtagaacg cctggcgcgc gaattcaatc
     5881 aactccactc tccactccct acgccccacc ccccagcacc ctcacctcat ccttcgtcaa
     5941 ctcgcgcatc ttcccgaccg gcaggtcgtc gatgcggatc gggccgatgg cgacgcggac
     6001 gagtttcagc acctctgcgt cgagcgcttc gaccatgcgg cgcacctggc ggttgcgacc
     6061 ttcagtgatc gtgatctcga agtttctctc gccgacgcgc tccacacgtg cgggacgtgt
     6121 tggtccgtcg cgcagctcga tg
//
LOCUS       DATLVW010000882         5013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_371431, whole genome shotgun sequence.
ACCESSION   DATLVW010000882 DATLVW010000000
VERSION     DATLVW010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371431"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..2840)
                     /gene="lon"
                     /locus_tag="VNN25_26035"
                     /pseudo
     CDS             complement(401..2840)
                     /gene="lon"
                     /locus_tag="VNN25_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            complement(2921..3367)
                     /gene="nrdR"
                     /locus_tag="VNN25_26040"
     CDS             complement(2921..3367)
                     /gene="nrdR"
                     /locus_tag="VNN25_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXH95061.1"
                     /translation="MRCPFCGNTEDRVVDSRESREADVIRRRRECTKCERRFTSYEKI
                     EQLPFQVVKRDERRETYDRGKLMRGLQVACRKRPVPQEKLERLADFIETAMQDSSERE
                     ISSKSIGTMVMERLRDLDPVAYVRFASVYRRFEDVDAFVKELHQLK"
     gene            complement(3383..4411)
                     /locus_tag="VNN25_26045"
     CDS             complement(3383..4411)
                     /locus_tag="VNN25_26045"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXH95062.1"
                     /translation="MRRTLQVTSVALLTIFFLGLFLWKSNLTDVWRIMTATSPWWLAS
                     AFAVNWMALIFRTIRWRVLLASPKPPAFYPTFFANTTGYMLSTVLPIRAGDVARPALL
                     SRRTNVRFAEALGTVLTERILDLISILTLFIWFAAVRWNAFPNGSGVVHGGALGCTIV
                     LIALLSFLVGLYFFGGVVRRVHEWIGRILPKRFREPWMKFFDAFAKTLAITERPRDFF
                     TVIFATAGVWLCLTAQYWLVFIAVHRPLPFDSTFFISAVTTIGVAIPTPGGVGGFHKL
                     CQWALTSFYGFDIDTSVATALLLHLVGTLPVVITGVVLFAREGLRWRDLRRAAEDVPA
                     DNETLSQS"
     gene            complement(4481..>5013)
                     /locus_tag="VNN25_26050"
     CDS             complement(4481..>5013)
                     /locus_tag="VNN25_26050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95063.1"
                     /translation="HKIFDVDALVKLDKRAGLKNLHVVNAPPKPPSPAPPPCSAAILD
                     ILGPPTNENTLSFELLGGTDSAIAFFLPKAMPPFSLTRSALRVVAPNTPQLDAAHSVL
                     GNLLDAYDTTRLYIAADVTQAETPLFAVPERGLRIPFVFSGTPRPGEVPPRFNVIQHL
                     RKEIAGGSTFVLVARE"
BASE COUNT          909 a         1506 c         1600 g          998 t
ORIGIN      
        1 cgaatgcgcc gggcctggac atgttgttgt gccgccgttc gctactccat tcaactcgaa
       61 tttcaacatc cagggagagg tcattcgctt catcacatgg tggcgacctt cgagacccac
      121 aggcccgctc gattctcgaa ggaggtcagc tttgaacact atcccgttgc cgtgattccc
      181 gagcgtttgg acccgaactt cagtctgctt acgctggtag gctaacttgc ccgtaatttc
      241 tcgttccgca gccagcttca gtcgctgatt cagcagcaag gacgaggcac acaacccctc
      301 ctacgcgggc aagaacccga cgactcgacg atccgctcgc agaccgcaat gaaggcgaac
      361 ctacgtctct accccggctg cgccaccggc acctgctctg tcactggcgg ggccggctcc
      421 ggctcggcca gcggcttcga ttcgcggccg gcccgttcgg tggtcttctg atcggcggcg
      481 gcgcggtcga gcagcgcaat cttcagcaca tcctcgacgt gctcgacgaa atagaactgc
      541 atgtcgcgct tgatcggctc ggggacgtcg atcaggtcac gctcgttcag cttcggcatg
      601 atgacggtcg tgatcttggc ggcgcgcgcg gccagcacct tttccttcaa gccgccgatg
      661 ggaagaacgt ctccacgcag cgtcacttcg ccggtcatgg caacatttcg tttcactggc
      721 cggccggtga tcatcgagat gatcgcgctc gtcatggtga tgcctgcgga cggtccgtct
      781 ttcggaatcg cgccggcggg tacgtggatg tgaatgtcgt gtgtctcgaa gtagtcggac
      841 gggatgttct gctcgtccgc atgcgcgcgt gcgtacgtga gcgccgcctg cgccgattcc
      901 ttcatcacgt cgccgagctg gccggtgagc acgagtttcc ctttgccttt cacggccagc
      961 gcttcgatga agaggatgtc gccgccgacc gcagtccagg ccagtcccgt ggccacgccg
     1021 acctggtcgc gttcgagcaa ctcttcggcg aaatgcttca tcggcccgag gtacttttcg
     1081 accgacgtgt cagtgatgcg gtagctctcc gcctggtttc cgcccgccac ctgcaccgcg
     1141 atcttgcggc agacggtgcc gatctcacgc tccagattgc gaagcccggc ttccttcgtg
     1201 tagccggtga tgatgcgggt gatgcctgag tcggtccaga ccaacggcgt ttctttgatg
     1261 ccgttctcct ccatctgctt cgggatgaga tgttgcttgg cgatggccag cttctcctca
     1321 tcggtgtagc cggacaggcg aatgacttcc atgcgatcga ggaatgccgg ctgaatcgtg
     1381 tcgagcacgt ttgcggtaac gacgaacatg acgttcgaca agtcgtaagg gacgccgagg
     1441 tagtgatcgc gaaaagagaa attctgttcg ggatcgagga cctcgaggag cgcggatgat
     1501 ggatcgccgc ggtaatcagc gccgatcttg tcgacctcgt cgagcatgaa gaccggattg
     1561 gaagtgccgg cctgattgat gccctggatg atgcggccgg gcaacgcgcc gacgtaggtt
     1621 cgcctgtggc cacggatctc cgcttcgtcc cgcacgccgc cgagcgagat gcggacgaac
     1681 ttgcggtcga gcgctcgcgc gatcgagcgt cccagcgacg tcttgccgac accgggaggg
     1741 ccgacgaaac agaggatcgg tcctttcatc ttctgcccgc gcagcttgcg cacggccagg
     1801 tactcgagga tgcgctcctt gatcttctcg aggtcgtagt gatcttcgtc gaggatcgtg
     1861 cgggcgcgca cgaggtcgtg gttgtccgcc gacatcacgc cccacggcag ccccgtcatc
     1921 caatcgagat acgtgcggat gatcgaggtc tcagccgaat caggatgcga ccgctccagg
     1981 cgcttgatct gcttctcgac ctcttccttc gcttcggacg ggatctgctt ctcttcgatc
     2041 ttcttgcggt agttctcgac atcctcggcg acgtcttctc cttcgccgag ctccgattgg
     2101 atcgccttca gttgctgccg caggaaatac tcgcgctgcg acttgttcat ctcgccctgc
     2161 gccgcggacg agatctcctg ctgcatcgtc aggagattga tctcgcgcga aaggaggtcg
     2221 ttgaccttct tgagccgctc gaccggatcc atcgtctcga ggatctgctg cgcttcttcg
     2281 agcttgaggt ctaggttcga tgccgccagg tcagtcaacc gcgccgggtc gtcgagattc
     2341 gccgcgatca ccatgacctc ggggctgatg ttcttgccga gccccaccgc ccggtcgagg
     2401 ttttgcttca cggcgcggat gagcgcctcg gtctcgaggc cgcgatcctt cgccgttgtc
     2461 tcctcgatgc gcgtgatctt cgctttgaag aacggagcgg tctggatgaa gtaatcgatc
     2521 cgcgctcggc tgaggccctg cacgaggatg cgaatgcggc catcgggcag cttcagcatc
     2581 cgcatgatga tggccacggt gccgactcga tagagatcgt cctcggccgg gtcttcgttc
     2641 tgaaagtcct tctgcgccgt gagcatgatg acgcggttct cggccagggc ctgatccacc
     2701 gcgttgatcg acttctcccg cgacaccgac agcggcacga tgatgaacgg gaagattacg
     2761 aggtccttga gcggcagtac cggcaggacg tcggggattt tgattggttc ttctttcgcg
     2821 gcttcgttcg cgtcagtcat tgatacctct cacatttcgg ggaaaccatc agtctggctg
     2881 tgcaatgcgt gttcctgtgc gctgcggttg ccaaacctcc ctacttcaac tggtgcaact
     2941 ctttcacaaa ggcgtcaaca tcttcgaatc tccgatacac cgaagcgaat cggacgtagg
     3001 ccacaggatc gaggtcgcgg aggcgctcca tgaccatcgt gccgatcgac ttactcgaaa
     3061 tctcacgctc gctggagtcc tgcatggcgg tttcgatgaa gtccgcgagt cgttccagct
     3121 tttcctgcgg gaccgggcgt ttgcggcagg ccacctgcag gccgcgcatc agtttgccgc
     3181 ggtcataggt ctcgcgccgc tcgtcgcgct tcaccacctg aaacggcagt tgctcgatct
     3241 tctcgtaact cgtgaagcgc cgctcgcact tggtgcactc gcggcggcgg cggatgacgt
     3301 cggcttcgcg gctctcgcgc gagtcgacga cgcggtcttc cgtgttgccg cagaacgggc
     3361 agcgcattct gaaggccgta tatcacgact gcgaaagagt ttcgttgtcg gccggcacat
     3421 cctcggcggc acgcctgaga tcgcgccagc gaagcccctc gcgcgcgaac agcaccacgc
     3481 cagtgatgac gacgggaagc gtgccgacca gatgcagcaa cagagcggtg gcaaccgagg
     3541 tgtctatatc gaagccgtag aacgaggtga gcgcccattg gcaaagcttg tggaatccgc
     3601 cgacgccgcc gggagtgggg atcgccacgc cgatcgtcgt cacggcgctg atgaagaacg
     3661 tcgaatcaaa cggcagcgga cgatgcacgg cgatgaagac gagccagtac tgggcggtca
     3721 gacaaagcca gacgccagca gtcgcgaaaa ttactgtgaa gaaatcgcgc ggccgctccg
     3781 tgatggcgag cgttttcgcg aacgcatcga agaacttcat ccacggctcg cggaatcgct
     3841 tcggcaggat gcgcccaatc cattcgtgca cgcgccggac aacgccgccg aaaaagtaga
     3901 ggccgacgag aaacgagagg agcgcgatca ggacgatcgt gcagcccaag gctccgccat
     3961 gcacgacgcc ggagccgttc ggaaacgcgt tccagcgcac ggcggcaaac cagatgaaga
     4021 gcgtgaggat gctgatcagg tcgaggatcc gctccgtgag cacggtcccc agcgcctcgg
     4081 cgaaccggac gttggtgcgc cgcgacagaa gcgccggccg cgccacgtca ccagcgcgga
     4141 tcgggagcac agtcgagagc atgtagccgg tggtgttggc gaagaacgtc ggatagaagg
     4201 cgggcggttt cgggctggcg aggagaacgc gccagcggat cgtgcggaag atcagcgcca
     4261 tccagttgac agcgaacgcc gacgcaagcc accaggggct cgtcgccgtc atgatccgcc
     4321 aaacgtccgt gaggttgctt ttccagagga agagcccgag gaagaagatc gtgaggagcg
     4381 cgaccgaggt tacctggaga gtgcggcgca aagtcgggca gttgtaccac agcgttgttt
     4441 gaagaatgag cgaacgggag gattgaactg acttgcctcg ctactcccgc gcaaccagca
     4501 cgaacgtact cccgcctgcg atttccttcc gaaggtgctg gatgacgttg aagcgtggcg
     4561 ggacctcgcc tgggcggggc gtgccgctga agacaaaggg gatgcgcagg ccgcgctccg
     4621 gaactgcgaa caacggtgtc tcggcttgcg tgacgtcggc ggcgatgtag aggcgtgtcg
     4681 tgtcgtaggc gtcgagcaaa ttgccgagaa cgctgtgcgc ggcgtcgagc tggggagtgt
     4741 tcggtgcgac aaccctgagt gctgaccgtg tcagggagaa cggaggcatc gctttcggca
     4801 ggaagaaggc gatggcggag tcagtgccgc cgaggagctc gaaggagagc gtgttctcgt
     4861 tcgttggcgg accgaggatg tccaggatcg cggcggagca tggcggtggt gcagggctgg
     4921 gcggtttggg cggtgcgttg acgacgtgca ggttcttcaa ccccgcgcgc ttgtcgagct
     4981 tcaccagcgc gtcgacgtcg aagatcttgt gcg
//
LOCUS       DATLVW010000883        31282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_36477, whole genome shotgun sequence.
ACCESSION   DATLVW010000883 DATLVW010000000
VERSION     DATLVW010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 31282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36477"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VNN25_26055"
     CDS             complement(<1..1017)
                     /locus_tag="VNN25_26055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95064.1"
                     /translation="MGERADVTILGSRVLLIAVAVTMLAAAAAFVVRPPEMGDVKTQP
                     DVRLLATRIAHHPTDWAAASALTEVALDARTDKRVMLWHAAYDHATRLAPERKDPPNA
                     FARAGFFHWAELPERDQRDVLSAYARLLHDPAVFGRMAGPIFELTGDLSYLRRNGPQT
                     ADGIGPLIALAVSNGRFADYRALRGELQRKRLDEFPARASTATPEELIDGFPSPPYHS
                     DTEPLIKALLNELHRHPLDAIPNRHDVAEGIADYALRHDLQPLDGLEVISRKPGAASV
                     ETQIALAKALGLKESALQLEMTSKDPRRVSPNDHEWQGGCGNDVCTRAWRVIDADHGI
                     ALTIQ"
     gene            complement(996..2060)
                     /locus_tag="VNN25_26060"
     CDS             complement(996..2060)
                     /locus_tag="VNN25_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH95065.1"
                     /translation="MLSDLAFHLAARGWRVEVVTSRQRYGDAAAGLSPRETVQGVAVN
                     RVWSTRFGRSFLPGRAIDYATFYLSAFVALFRKARRETVIVALTDPPLISVVAAIAAM
                     LRRATLVNWTQDLFPEVAEALGMRALRVLRGIRNWSLRRARTNVAISESMAARLPNAV
                     VIHNWADASLCPVDDRHDRFIVGYSGNLGRAHDASTMLAAMRALRDDSTIEFLVTGGG
                     AQLDVIRREHLPNVRFAEYAPREQLSESLSAVDAHLVTQQPSLEGLVVPSKFHGILAV
                     ARPVLFAGSANGELARIINTHDCGMVVESGDGAGLARCVRELATNPERARAMGERGRS
                     LYETMYAPPIAMAAWENVLT"
     gene            3266..5011
                     /locus_tag="VNN25_26065"
     CDS             3266..5011
                     /locus_tag="VNN25_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXH95066.1"
                     /translation="MIILGINAHHADASAAIVVDGKLVAAAEEERFRRIKHWAGFPSV
                     AIRYCLDEARVDLNDVDHIAVNRDPNARLFQRAKFILAKRPSIGAIRKRLANRAKIVS
                     IDDELRQAFSVETLKPVLHHVEHHRAHLASAYFVAPFDDAAVLSVDGFGDFVSSMWGV
                     ASRNKIEVLGEIGFPHSLGIFYTAMTQYLGFPHYGDEYKVMGLAPYGKPAQIGAMRDI
                     VHVQNDGTFRLNLDYFTHHSAGLDMVWQGGMPVIGPVYSQHLIDRLGPMRAPDDPIEQ
                     QHKDLAASMQAMFEEAFFALANALYEKTKNPRFCLAGGCGYNSVANGMVFDRSRFRDL
                     YIQAAAGDAGGAIGAAYWVWNEILGSTKSFVMTHAYWGPEYDDVAIDESIEARRADLD
                     AAGCTIERVSDERELCRRTAAQIAEGDVVGWFQGRMEWGPRALGNRSIVCDPRRADMK
                     DILNKKIKRRESFRPFAPSVLREAVGTWFETDYDVPFMLQVYQIREEKRALIPAVTHV
                     NGSGRLQSVDEDQNARYHHLIEAFRDVTGVPLVLNTSFNENEPVVNTPAQALDCFLRT
                     KMDLLVMGDIMIRRQ"
     gene            complement(5066..5287)
                     /locus_tag="VNN25_26070"
     CDS             complement(5066..5287)
                     /locus_tag="VNN25_26070"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HXH95067.1"
                     /translation="MEVIEAEFNDGVLRPMRRLPLRPGERVGIVLVRRPDPLRWDLAR
                     LSKTSPDENTLTEAGLSEWESALSHEDRR"
     gene            complement(5419..5988)
                     /locus_tag="VNN25_26075"
     CDS             complement(5419..5988)
                     /locus_tag="VNN25_26075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95068.1"
                     /translation="MSTEVPVTPEQSHTDSAQARIEEIRAIVQKIPNLVIPTSKGAGQ
                     RLIATASVPKKFVELTAVAVRNSAPLVRQSGQDLEQDRDRTSYAEAYAPVADELEAQA
                     HFVRHSVAVAKSEVGSSALTTYAMARLLAKRPETADLLPHVEDMSKALGRRGRNSKSK
                     PAPKAATPVTTTETSAVTASSSTTNPKTK"
     gene            complement(6026..6715)
                     /locus_tag="VNN25_26080"
     CDS             complement(6026..6715)
                     /locus_tag="VNN25_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95069.1"
                     /translation="MATAENRSGSQRNANHSRRAGLRSCGSDRDRRKSWTRPFRRTRR
                     AIESSLDLISACRRVIEATESLAAERPIRAARRIQIASGWLDRALKQLDCAAAGLRKT
                     RNRAAQSPELALDAPAKLAEVSVQWMKAAGQLAAFSKRLEDTSTWLFDGVMGGSIAIP
                     IEEQGANAGKRVPIRLIPPPLRPPNWFPFESNDAPCIPARRRSVCLTVVEASRRIFRG
                     RAPPLVSTCSL"
     gene            6833..7123
                     /locus_tag="VNN25_26085"
     CDS             6833..7123
                     /locus_tag="VNN25_26085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95070.1"
                     /translation="MSFRHRNRLRRSQNLANDNQILVINNQVPVINNQILAINSQILV
                     INSQILVINSQILVINNQILVINSQILVMNNQILVISNQILVNDWSERRVTA"
     gene            complement(7113..8168)
                     /locus_tag="VNN25_26090"
     CDS             complement(7113..8168)
                     /locus_tag="VNN25_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXH95071.1"
                     /translation="MRAAVFHGVDDIRVEDVPRPRAGVGEAVIRVTMTTICGTDLHIL
                     RGEYPVKPGLIVGHEPVGVIEELGAGVTGYKIGDRVIVGAITPCGQCNACLSGNLSQC
                     GHGSGYEAIGGWRFGNTINGAQAEYLLVPSAQANLAKIPDGVTDDQVVLLTDIASTGI
                     SGAESGGVKIGDSVAVFAQGPIGLCAALGAKLMGAAVVIGVDGDDNRIATARKMGVDI
                     VLDYRTTDVIAEIRKLSGGGVDVAIEALGTQATFENALRVLRPGGTLSSIGVYSHNLQ
                     VPYDAYAAGLGDHRIVTTLCPGGKERMRRLIEMVRRGKLDLTPLLTHRFKLADINAAY
                     KLFGERADGVMKVVITP"
     gene            8335..8739
                     /locus_tag="VNN25_26095"
     CDS             8335..8739
                     /locus_tag="VNN25_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXH95072.1"
                     /translation="MSLRTYQAGSPRKPGEGLRIGTVRFPPRGVRKEDYARLDFFDVW
                     FPVVAPSRELLAWVKKHDIANDAASMRTFQKRYARELESDPAARYSVLLLAALAQQTA
                     ISIGCFCEDENNCHRSILKTVIEKAAAALPER"
     gene            8748..10214
                     /locus_tag="VNN25_26100"
     CDS             8748..10214
                     /locus_tag="VNN25_26100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95073.1"
                     /translation="MLRRLYFGLALLALAPIWSASYLPTSDGPSHLYNSWVLQQLVRG
                     SHGPVADWYAIDWRPLPNWLGHAVMALLMMVVPPLIAEKLLVSGIVLLFLYAIWRYAG
                     AADESRQLFAFLAFPFAYNLLLQQGFYNFCISAALYFLVLAVWWKRRDTPDARTIAVV
                     AGLLLLCYFSHPMSTVLAIGSIGILWLATLRGRPLAIHARHLVALLPVLPLLAWFFRV
                     QGVSLARAPVSAAGLFSYIMRMWVLLTFDEFQAKLGFALFLALAGLIIITLIRRRWRW
                     SEGDAFILVTLALVVVYARAPAASSGGTMIMERMALFVVLSPIAWLAPRLPRRATIAL
                     VIVLSVVSVAYTGYLVRRYRGLSRRVTELVRSADAIGRGTAFVPLIREVRPHGSFVAV
                     QMHAIDYAAIEKGSVDIANYEAAAGFFPIRYRSGIAPPDTNTAGPRASEVDLTAYLAR
                     VPYVFLWHVPDDAPVMSQLATCCERVGASNGGRVYLKH"
     gene            complement(10265..10717)
                     /locus_tag="VNN25_26105"
     CDS             complement(10265..10717)
                     /locus_tag="VNN25_26105"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXH95074.1"
                     /translation="MNKRWIPLMAIALLVAAIASAQLAGAPPQRGGRMGALLVQVLQP
                     TPDQLATWKQLREAQATAMKPLVQNARDLRDQLDTAMKATPPDPATVGKLTLSLRAAR
                     QAIRALAGDSEAKFVATLTPDQKTKFEALQAAGKAFRHPHAMGGMGQR"
     gene            complement(10910..12109)
                     /locus_tag="VNN25_26110"
     CDS             complement(10910..12109)
                     /locus_tag="VNN25_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH95075.1"
                     /translation="MANQAKRLHTKAIHGGLDPAAHRGAVSVPIYQSSTFAFPTAEEG
                     AARFDGTSDGPIYTRMGNPTVHALEECVADLEGGCGAVATATGMAAISTVFLALLRAG
                     DHVVATDPLYGASRGLLTRYFSRLGVTSTFVPATDAAALTAAIRPETRLIYVETPANP
                     TLDLVDLIAASATARGAGIPLVVDNTFAGPYLQRPIELGADVVLHSATKSLNGHSDVI
                     AGVVVARDPSMLKTLRDAAIMFGLTIDPHQSWLLLRGIRTLGMRVERAQTNALSIARW
                     LEDHDQVAWVRYPGLPSHPQFKLAQQQMDGPGSIIAFEMRGGVTAGSILMNSVRMITL
                     AVSVGGVESLIEHPASMTHKSVPESEQRRQGITAGLVRLSVGCEDLDDLLADLTQAMA
                     HVTAAVG"
     gene            complement(12271..12591)
                     /locus_tag="VNN25_26115"
     CDS             complement(12271..12591)
                     /locus_tag="VNN25_26115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXH95076.1"
                     /translation="MNRKLLSIMILALLVASVSFAAGPGAADTYKSKCATCHGPDGKG
                     ATSIGKSMKIRDLSSADVQKQSDTDLQKIISDGKGKMPAYKTKLSVADVSSLVAFIRG
                     LAKH"
     gene            complement(12713..15757)
                     /locus_tag="VNN25_26120"
     CDS             complement(12713..15757)
                     /locus_tag="VNN25_26120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95077.1"
                     /translation="METGKHRHHRILLVLLAGILILGVAGGAWAAAPVTVTTAITGDP
                     VPGATVTAKANVVINDGSSLVSIKWTQNGGPAATLSNTGTDTVTVALPDRKVFREALL
                     TALEEAPIADSQYPANIPVTPFEGGLQNRFTVASVSPHALADSAAVKFDVTVTTTSGT
                     YHFAATVAGNTPWSTATGTRNVPILLPVLLHGKTQAQYNWSLSGPTGSTATLLDPTSQ
                     NPEFTPDVAGTYQLTILDLATAKPVTITVHAGTWKGIITGQDATGRPVADAACMGCHV
                     KNTPHFDLFTPWAKSGHAEIFTQNVTTPAGHYSSSCLSCHTVGFNAKPVQNNGIDEAS
                     DWKAFLATTLLTVGTPTNWTTILANYPATARMANIQCENCHGPQDSVAHMKKDGSRMS
                     LSSDVCGSCHGEPTRHGRYQQWQLSKHADFPTAVAEGMDPTCSKCHSAQGFVAWQDTG
                     FSTANLNVTWTADEVQPQTCAACHDPHDVGTTSSDKNTNAKVRVTGTTPLLMAGFKAT
                     SVGTGAVCMTCHNGRRDLRDDSHFTVADATRAPHEGPQADIVMGQNLYFTKVGTRGLH
                     SMIQDSCVSCHMESTDPPAALALQNADGSYVGTNHAFAASDTICNKCHTNITKDTVQA
                     PVQAKMDTLKAQMETAIKNLMVTQIRAGNSIDLNGLKTIKNASDISGVEFISSHGRQG
                     VNVTLTGGTKVSDLSLATVKIVRPGGTAVELYSVADPSIAKAGWNYFMVLADKSMGVH
                     NPAFVNSALDISNFAVSSFNASATATPGPGSVNNSAIGGGLGNGAGAVSCKSNYVYWS
                     EMVGHTAGQAGSQWRTDLVARNLESSTASLRFILHQASGDLEATGTVNGNSQKAFEDL
                     TAIMGPSNIGALEVCSDRPLLVATRIFNQADNGTYGQSYDGRVADLGYTVGQTISLIG
                     LRQKTDAYRSNLVVTNGGKTEAQVAINLFDANGKSLTSYNLTVPVGSGVQDLEPFKNR
                     ANAPDLDWGFATVTVLKGTNVLSSASLIDMKTNDPTTIPAKQ"
     gene            16163..19120
                     /locus_tag="VNN25_26125"
     CDS             16163..19120
                     /locus_tag="VNN25_26125"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HXH95078.1"
                     /translation="MQKTDAPGVALPADLTGWLPAAQRFIEWLVEFKHWNTPEAWLAR
                     GVIWVVVVYSLLRLIRGVLELLVQLKTYWTGLGWKVRRLTEEERAQLNRRQRFARVLS
                     SDLSNMAKVENWNDQFYTDLEAQIEAEGRFYATNFDRIIRRASEGLRRVSSLVRAIES
                     SAEQFLLLIGEPGSGKSIALRHLAHQLAESALTSKAKMVTIPLYVNLKELPSPTSGDP
                     NSDYIKAFVLENVRRGDADTAEYIRDNWAKHRDDGSWLFLFDSFDEIPAVLHSPSGSR
                     AATLYSEAIRQFLSTMGSCRGVVASREFKGPRNFGWHRLRILAMNEDKQALLVANSYL
                     PSERRRMVMQHIATSESTMLQNPMYLTLLCRFVRDANALPRNDQEVLERHIVRLAERD
                     ADHVGMKYALTTRQLLQGASTLARLFAEQQDLSLAPTITDIRLALSDSDVPGGSIDNV
                     IGALVDVKIGRSDVPEARIGDRRFTFAHRRYQETLFVQFLAANPDYIAPKRLLTETRW
                     REYAVTFLQTQPVETLQSLIRGAIDLLKEYDKRQSYVAVLPEYGSTAVYHRWDDAIPR
                     LLGLLEEGLAGRPNDVPADLRAAVAAVLMPRWHDGDFMDRRNVLRIAGLLPPELLTEI
                     LVETVRGGSHDTLDIALRKTVFLSNIPPELSRWIQRRLSDDTLDAENDSEITRLEALA
                     ARLPQSVRAAFIVSNCKRLRRRMILCTIPMFWPNVALLALITVLLDKSVRAARRRAEQ
                     VMTEMAVLNAIVAVSACAVVVVGICWRMRGGATIASAVHFTSAIALIYGLVGLVVLAQ
                     YSSRASGKHISPRACLKAMVGLSSEMRRPGQVAKGVVFLATGVMFGMMILDISLVGLW
                     GSRGWLTALVVFVVMVAIEPAIIAIRRRRFRQAYSVYAVSKRCPVAVALHATSLRALV
                     DWTKQETLLRSVRDVRSLGRILRCEPLTGIKPKCKHPIRVQFLSEEREFDLREIGARL
                     NHLADRLTKAGRP"
     gene            complement(19532..19753)
                     /locus_tag="VNN25_26130"
     CDS             complement(19532..19753)
                     /locus_tag="VNN25_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95079.1"
                     /translation="MTIKRLDHASVAVDDLTAAIVLGTGLELTKFTNPKLIEIESAIS
                     PPNSLGLRSVMFTVESVDDTVARLRRRAS"
     gene            19819..20247
                     /locus_tag="VNN25_26135"
     CDS             19819..20247
                     /locus_tag="VNN25_26135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXH95080.1"
                     /translation="MNDVIKVAESDAEIDSCFPVMSLLRPHVQRAEFVRLVRKMETQG
                     YRLAYLTADDRIQAVAGYRIIDMLRTGPMLEVDDLISAADARSKGYGKQPLDWCYDLA
                     RRSSCSVVELDSAVHRADAHRFYFRERMHVLAFHFSRTVV"
     gene            complement(20310..20729)
                     /locus_tag="VNN25_26140"
     CDS             complement(20310..20729)
                     /locus_tag="VNN25_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH95081.1"
                     /translation="MTMNPVVHFEMPAEDRKRMANFYQKAFGWQAQLLGPDMGDYVVV
                     TTTESDDNGPKKAGAINGGFYPKSSERPAQYPAVVIAVDDIQQSMKTVSDAGGKVLGD
                     PMDIPGVGKYVAFFDTEGNRASMLQPLERMSVRPKSK"
     gene            20851..21276
                     /locus_tag="VNN25_26145"
     CDS             20851..21276
                     /locus_tag="VNN25_26145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXH95082.1"
                     /translation="MKRIALLSLLMFACRDTHQHGVTIARSGKAAARIVVLTIGVAIK
                     ETGPAETVKKFGEVYAFSPEVFAVRRDQPTEIVFWNLQPDDEHDFMLTDPANKVLLQT
                     KFPPLSKTSFVMTFHDAGVFPFYCTMHQPAMSGQVIVSP"
     gene            21316..21948
                     /locus_tag="VNN25_26150"
     CDS             21316..21948
                     /locus_tag="VNN25_26150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95083.1"
                     /translation="MRATIAVTIALIAHTGFAGTVKRCGVSFVLPAKWTAVEKVSPDY
                     PDETDIQCRVALRPEGWSARAKRSRWDASDPPVWLMIFGAGTSFEDALDGAGFENEFE
                     EGGGRLGIPGRFGFSEAEPYSAGRFSGLVVRPTFRGFIRDVSTLRENESGLSTRDNES
                     VVMKEPSGRIIGFASDGGTPDEPIDRDAIIAGIAQTLVFEAPKSNEPQRH"
     gene            complement(21997..22443)
                     /locus_tag="VNN25_26155"
     CDS             complement(21997..22443)
                     /locus_tag="VNN25_26155"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH95084.1"
                     /translation="MLVKEVMTPAPVCCTPDTKIDQVATLMLDHDCGIVPVCKDSKLV
                     GVITDRDVTCRAVAKGMYPAAVAVRDVMTKTVFTIHQDEEVDAAIQLMEARQVRRLPV
                     VDDAGHVVGIVAPSDLAPTFASTNVADFLLAVSYWSRRPAAAPAVS"
     gene            complement(22670..23950)
                     /locus_tag="VNN25_26160"
     CDS             complement(22670..23950)
                     /locus_tag="VNN25_26160"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HXH95085.1"
                     /translation="MSTATLLLKGKEYELPVMEGSEGETAIDITSLRTKTGAVTFDPG
                     YGNTGSCASAITFIDGEKGILRYRGYPIEELAAKATFVEVCYLLIHGTLPSPQELDAF
                     NEKLTRHSLIHEDMKKFFEGFPPSAHPMAILSAMIASLSAYYPENDNELNIIRLLSKA
                     KTIAAFSYKKSIGQPFIYPRNDLSYTQNFMHMMFAVPAEGYKVTAAIDRALNLLLILH
                     ADHEQNCSTSTVRMVGSSGANLFASISAGICALWGPLHGGANQQVIEMLQQIRESGQS
                     ARTFVERAKQKDSGFKLMGFGHRVYKNFDPRATILKRAADDVLSALGKRDPLLQTATE
                     LEQIALSDPYFIDRKLYPNVDFYSGILYRAMGIPTNMFTVMFALGRLPGWIAQWKEMV
                     DDDKTRINRPRQLYVGSKVRELSEVNEELVLTTA"
     gene            complement(23979..25310)
                     /locus_tag="VNN25_26165"
     CDS             complement(23979..25310)
                     /locus_tag="VNN25_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HXH95086.1"
                     /translation="MSEHVIDTVLRVRVEHRAGQLARLANTIAEAGGLIGDVSTIQVG
                     ETATLRDVTIETIDEAQTIHVVRCIGEIDGVDVVESTDRVFEAHRGGKLHSTSRVHLD
                     QLADMRSIYTPGVARVSQAIVKDPNRAWDLTSVGNSVGIFTNGSRVLGLGNIGPLASM
                     PVMEGKAVLYDELVGISATPILIDTLDPLEFVETVMRVSPTFGGIHLEDIRIPDCYVI
                     EDELRRRLCKPVMHDDQHGTATVTLAAVINACKITGRDLKRSRVGMIGLGAAGSAIAM
                     LAVSYGVGEVLVSDINPDAVSRVQRAGAIASDAATIMATADIVIAATGRPGLIDASDI
                     HNGQVIFALTNPKPEILPSEARAAGAAFAGDGRSINNALAFPGLFRGALEARSCAITT
                     EMLIAAAETIASLAKPGDVVPSCLDRNVHQAVAATVAETARNAGLAGTAKL"
     gene            complement(25577..26041)
                     /locus_tag="VNN25_26170"
     CDS             complement(25577..26041)
                     /locus_tag="VNN25_26170"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXH95087.1"
                     /translation="MNFLTRFERWDPFEELQTLRGRTDRLWAQLTQDKTPLADWAPKT
                     DVIETTNDIVLRAELPGMNEKDVDIQIENGVLTIKGERTAEKELEEKGYRRIERDYGT
                     FFRSFTLPVNIDAEKAAATFANGLLEVQLPKKEGAKPKTIKVEPKKLLTKAA"
     gene            complement(26250..26441)
                     /locus_tag="VNN25_26175"
     CDS             complement(26250..26441)
                     /locus_tag="VNN25_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95088.1"
                     /translation="MRKTLFWCSWILLIALPVIYGIQIYISQDLPEVQPWKWAILFGS
                     VVVIYFSRNTDDVLKHHLA"
     gene            complement(26524..27249)
                     /locus_tag="VNN25_26180"
     CDS             complement(26524..27249)
                     /locus_tag="VNN25_26180"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXH95089.1"
                     /translation="MRRSFVIALLLAGAAVTLRAGGRVSAAQEFPVGATIPTIDLIDD
                     TGRTRSTAEWRGTPTILAPMYARCPIACPLIVQGLKSGTAESSADPSTYRVVIFSFDA
                     RDTPADLHRFRVRHNLPLAWTVVMAKPADTRRLLDAIGYRVGDANGLLAHPNVVVALT
                     PDLKTAKYLAGTTYRGGEIDEALNIARGGRDWIAQSGGWVLAILLLVCALSAVHLFTL
                     FGARPPGAGRPSIAAPPQRHSNA"
     gene            complement(27246..28946)
                     /locus_tag="VNN25_26185"
     CDS             complement(27246..28946)
                     /locus_tag="VNN25_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXH95090.1"
                     /translation="MKSAVQPYSRTDVEPGLTKIILFEIVFPMILLILGITMGLLQVL
                     FRAGIIKSQSFLGINYYQGLTLHGAINAIVFTTFFAVAFGHAVIRFYTGKPLLLGAAW
                     WSLILMTGGTIMAAIPMLTGSASVLYTFYPPLKAHPAFYIGLVLLVIGSWVAFFSWIP
                     VFQKWKRENPATKMPLAVLGVFATFIVWFIATIPVAIEIIVLLIPWSLGWTQTVNVPL
                     ARMLFWFFGHPLVYFWLLPAYVMYYTMLPKLTGGKLYSDFAGRFTFMWLIAFSAPIGI
                     HHQYTEPGISTTWKFVHAFFTMLVAIPSLVTAFTLAASMEHGARKSGGTGLFGWWKKL
                     PWLDTDRWMFAYLFCGLFIFIFGGITGIINASYSLDNVVHNTAWMPAHFHQTVAGPVF
                     LAFIGGSLLLISTLGGKEIALKRLNVWIPYLWTLGIMVFSTGLFIGGIAGEPRRTNMG
                     LSYTNPASPLYQPSWNVARVAGTIGGVIMFLTVLLYFVIFFGTLLRKKSSAGVLELPV
                     TEPYHDEDIVALQSLRPWLVATVILLIVAYTMPFVELAKARYEGAPRYSPASPVALRK
                     "
     gene            complement(28963..29730)
                     /locus_tag="VNN25_26190"
     CDS             complement(28963..29730)
                     /locus_tag="VNN25_26190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXH95091.1"
                     /translation="MTTATIGIEKRIVVTAALFVLTLTGVIAMSVKVFGFGLPTCLTD
                     VRPFRDGKVITQAPKRYEVHYLARMWRFEPADISIPPGSTADIYLSTADVTHGMQIIG
                     TNINLMAVPGAVNYARVKFDQPGEYLVVCNEYCGIEHHQMAARIHVTDRAAAPPPPPV
                     AEAALGGQELLDKYACTACHSLDGSPMPGPTFKGLYGSKRTLADGTTAVADDAYLLES
                     IEKPNAKTVKGFDPLMPETTIPPAEMRAIIQFIKTVK"
     gene            30114..31262
                     /locus_tag="VNN25_26195"
     CDS             30114..31262
                     /locus_tag="VNN25_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HXH95092.1"
                     /translation="MSPKEGALSLPPTIAQQMKRSGTSRRDFMTLCGKLMVAAPFGLA
                     LTNFTSPNAVAAVIGRARRPSVIWLHMQDCTGCTETLLRTSEPALDTLLFDIISLDYH
                     ETVMAASGADAEAALHDAMTQNDGKYVLVVEGSIPTKENGNYLKIAGRNGIDMLKDVA
                     SHSAAVIAIGSCSSWGGVPSSGPNPTGATGVDSIIKDKPVINLPGCPPNPYVFLATVL
                     EYATAGKVPKLDSQGRPKFAYDRLIHDHCPRRAHFDNGRFAAQFGDAGHIQGYCLYKL
                     GCKGPVTHAPCSTRHFNEVVDCWPIGIGAPCVGCTEKDIAFNVPIFQTADMKGQAPPA
                     FYPEVSPEHKGMSPMATGVAGLVGGALVGAGYIASRKLDNTSGEQPKE"
BASE COUNT         5817 a         9326 c         9843 g         6296 t
ORIGIN      
        1 ctggatcgtc agcgcgattc cgtggtcggc atcgatcact ctccaggccc tggtgcagac
       61 atcattgccg caccctccct gccactcgtg gtcgttcggt gagacacggc gaggatcctt
      121 ggacgtcatc tcgagctgga gtgcgctctc tttgaggccg agcgccttcg ccagcgcgat
      181 ctgcgtctct accgatgccg cgcccggttt gcgggagatc acttccagcc cgtcgagcgg
      241 ttggagatca tgacgcagcg cgtagtcggc gatgccctcg gccacatcgt ggcgattggg
      301 gattgcatcg aggggatggc gatgcaattc gttgaggagc gctttgatca gcggctccgt
      361 atcgctgtga taaggcggac tcgggaaccc atcgatgagc tcttccggcg ttgctgtcga
      421 cgcgcgcgcc ggaaattcat cgaggcgctt ccgttgcagc tcgccgcgca gcgctcgata
      481 gtcagcaaac ctgccattcg acactgccag cgcgatcaac ggcccgatgc cgtccgccgt
      541 ctgcgggccg ttcctccgga gataggaaag atcgcccgtc agctcgaaaa tgggcccggc
      601 catccgtccg aagaccgccg gatcgtgcag gagtctggcg tatgccgaaa gaacgtcgcg
      661 ctggtcccgc tccggcaatt ccgcccagtg gaagaacccc gcacgggcaa aggcattcgg
      721 cggatctttg cgctccggcg cgaggcgcgt ggcgtgatcg taggcggcgt gccagagcat
      781 cacgcgcttg tccgttctgg cgtcgagcgc gacctccgtc agcgcgcttg ccgccgccca
      841 gtccgtcggg tggtgcgcga tgcgtgtcgc cagcaggcgc acgtccggct gcgttttcac
      901 gtcgcccatc tccggcggcc tgacgacaaa cgccgccgcc gctgccagca tcgtcacggc
      961 cacggcgatc aggagcacgc gcgatccgag gatggtcacg tcagcacgtt ctcccacgct
     1021 gccattgcga tcggcggcgc gtacatcgtt tcgtagagcg accggccgcg ctcgcccatc
     1081 gcccgtgcgc gttcgggatt cgtggccagc tcgcggacgc agcgcgccag tccggcgccg
     1141 tcgccggatt cgacaaccat tccgcagtca tgtgtattga tgatacgcgc cagctcgcca
     1201 ttcgcgcttc cggcgaacag cacgggacgg gcgacggcga gaattccgtg gaacttgctg
     1261 gggacgacca aaccctcgag cgatggctgc tgcgtgacga gatgcgcgtc cactgccgag
     1321 aggctctcgc tcaattgttc gcgtggcgcg tactcggcga agcggacatt ggggagatgt
     1381 tccctccgaa tgacgtcgag ctgcgcgccg cctcccgtga cgaggaactc gatcgtgctg
     1441 tcgtcgcgca acgcacgcat cgcggcgagc atggtgctcg cgtcgtgtgc gcgcccgagg
     1501 ttgccggaat agccaacgat gaaccggtcg tgccggtcat cgaccggaca gagcgaggcg
     1561 tccgcccagt tgtgaatgac cacagcgttc ggaagccgcg ccgccatcga ctcgctgatg
     1621 gcgacattcg ttcttgcgcg ccgcagcgac cagttgcgga tgccgcgcaa tacgcgcagt
     1681 gccctcatgc cgagcgcctc ggctacttcc gggaagagat cctgcgtcca gttgacgagt
     1741 gtggctcgcc gcagcatcgc cgcgatcgcc gcgacgacgg agatcagagg cggatcggtc
     1801 agcgcgacga ttaccgtttc ccgccgcgct ttccggaaca gcgccacgaa tgcgctcaag
     1861 tagaacgtcg cgtaatcgat ggcccggccg ggaaggaagc tccgcccaaa ccgcgtggac
     1921 cagacgcggt tcacggcgac tccctgcact gtttcccgcg gcgaaagtcc agccgccgca
     1981 tccccgtagc gctgcctgct cgtgacgact tccacccgcc acccccgcgc agccagatga
     2041 aaagcgaggt cggacaacat ctgacttgtt gcagaatgat caggatggaa gaagcgattc
     2101 acgaagatga cgtgtcgcgc gcacatgcgg atgcggacat tatgcggccc ccgtcctggc
     2161 ggcgactgga gtcttcgcta gtccccattt ctcgtccgtt tacgcgactc cctcacatcg
     2221 agcgccttct ccccccgggc tttttcgggg ggagaaggtg ccgaaggcgg atgagggggg
     2281 cgttgagaaa tgcgatacgt acgttctttc cgagagccgt gagcaatgcc gcgatagtca
     2341 aacgccccct catccggcct tcggccacct tctcccccac aaaaagcgtg ggggagaagg
     2401 cgctcgatag aacgatgctg ccaaggaatc cctacaagtg tgagaaatcc aagctaggtc
     2461 gtatttctca cagttctcag cgactccttc acatcgagcg ccttctttcc ccgggctttt
     2521 tcggggggag ggtccgacgg cctgcggccg accgccgtgg acgaagattg cacccgcggc
     2581 tgaatacacg cacagcgaac ctactgaagg ttcgcgctca cgcaaaatgt ctgcgactaa
     2641 aacgtcgcaa agggggacaa acattgtact tgcgcctgaa aacgatgtcc acccacccac
     2701 gcgatcttca gagcagaagg tgccgaaggc ggatgagggg ggcgttgaga aatgcaataa
     2761 gcacgttctt cccgagagcc ctgagcaacg atgccgcgat agtcaaacgc cccctcatcc
     2821 ggccttcggc caccttctcc cccacaaaaa gcgtggggga gaaggcgctc gatggaaaga
     2881 tgctgccgag gaatctctgc aagggtgaga aatgcaggct gggtcgtatt tctcacaatt
     2941 ctcagcgaca ccttcacatc gagcgccttc acaattctca gcgactcctt aacatcgagc
     3001 gccttctccc cccgggcttt ttcgggggga gaaggtgccg aaggcggatg aggggggcgt
     3061 tgagaaatgc gataagcacg ttctttccga gagccgtgag caacgatgcc gcgatagcca
     3121 aacgccccct catccggcct tcggccacct tctcccccac aaaaagcgtg ggggagaagg
     3181 cgctcgatgg aaagatgctg ccaaggaatc cctctaaggg tcataaatca acgcttctcc
     3241 aaccaccacc cccggtcgcc ccccaatgat catcctcgga atcaacgccc atcacgccga
     3301 cgcctcggcg gccatcgtcg ttgacgggaa actcgtcgcc gccgccgagg aagagcgctt
     3361 ccgccgcatc aagcactggg ccggtttccc gtccgtcgct atccgctact gcctcgacga
     3421 agcgcgtgtc gatctgaacg acgtcgacca catcgccgtc aaccgcgatc cgaatgcgcg
     3481 gctctttcag cgcgccaaat tcatcctggc caagcgtccc tcgatcggag ccattcggaa
     3541 gcgtctcgcc aaccgcgcga agatcgtcag catcgacgac gagctgcggc aggccttcag
     3601 tgtcgagacg ctgaagccgg ttctgcatca cgtcgagcat caccgcgcgc atctcgcctc
     3661 ggcatacttc gtcgcgccgt tcgacgacgc cgcagtgctc tccgtcgatg gcttcggtga
     3721 ctttgtcagc tcgatgtggg gcgtcgcgtc gcgcaacaag atcgaggtgc tcggcgagat
     3781 cggattcccc cactccctcg gcatctttta caccgccatg acccaatacc tcggcttccc
     3841 tcactacgga gacgagtaca aggtcatggg cctggcgccg tacggcaagc ctgcgcagat
     3901 cggtgcgatg cgcgacatcg ttcacgtgca gaacgacggc acctttcggc tcaacctcga
     3961 ttacttcacc catcactccg cgggcctgga catggtctgg cagggaggaa tgcccgtcat
     4021 cggtcccgtc tattcgcagc acctcatcga ccgcctcggc ccgatgcgcg cgcctgacga
     4081 tccgatcgag cagcagcaca aagatctcgc ggcgtcgatg caggccatgt tcgaagaagc
     4141 cttcttcgcg ctggcgaacg cgctctacga aaagacgaag aatccgcgct tctgtctggc
     4201 cggcggttgc ggctacaaca gcgtcgccaa cgggatggtc ttcgaccgct cgcggttccg
     4261 tgacctctat atccaggccg ctgccggcga cgccggaggc gcgatcggcg ccgcgtactg
     4321 ggtctggaac gagatcctcg gcagcaccaa gtcgttcgtg atgacgcacg cgtactgggg
     4381 tccggagtac gacgacgtgg ccatcgacga atcgatcgaa gcgcggcgcg ccgatcttga
     4441 cgccgccggc tgcacgatcg agcgcgttag cgacgaacgt gaactttgcc gccgcaccgc
     4501 cgcacagatc gcggaaggcg acgtggtcgg ctggttccag gggcgcatgg agtggggccc
     4561 gcgcgccctc ggcaaccgct ccatcgtctg cgatccgcgc cgcgccgaca tgaaggacat
     4621 cctcaacaag aagatcaagc gccgcgagtc gttccgcccc ttcgctccgt cggtgttacg
     4681 cgaagccgtc ggcacctggt tcgagaccga ttacgacgtc cccttcatgc tgcaggtcta
     4741 ccagatccgc gaagagaaac gggcgctgat tccggcagtc acgcacgtca acggatcggg
     4801 acgtctgcaa tcagtcgacg aagatcagaa cgcgcgctac caccatctca tcgaagcctt
     4861 ccgcgatgtc accggcgttc cgctcgtgct caacacttcg ttcaacgaga acgagccggt
     4921 cgtcaacacc ccggcccagg ctctcgactg cttcctgcgc acgaagatgg acctgctggt
     4981 gatgggcgac ataatgatcc ggcggcaatg agctgctccg gaacaaatgc gcgggcgcgc
     5041 ccgcggggtc agatctcacc gcgcttcacc tgcggtcctc atgactgagt gctgattccc
     5101 attcagacag gcctgcctcc gtcagagtgt tttcatccgg actggtcttc gataaacggg
     5161 caaggtccca gcgcaaagga tccgggcggc gcacgagaac gatccctacg cgctcacccg
     5221 ggcgcaatgg taggcggcgc atcggacgaa gcacgccatc attgaactcc gcttcgatca
     5281 cttccatcca gtcaaacggt aacacggaag ttgaggttga taaggaaaag gaacgacaga
     5341 tcgggcgact atcgctgaaa tgcgcgggcg cgccttcgga cgcgcccgcg cggactactt
     5401 cttactgcac gacaccagtt acttggtctt cggattggtc gtcgatgacg atgccgtcac
     5461 cgccgaggtc tccgtagtcg taaccggggt tgccgccttc ggggccggct tggacttgga
     5521 attccgtccg cggcggccga gtgccttgct catgtcttcg acatggggaa ggagatcggc
     5581 ggtctcggga cgcttggcca gaagacgcgc catggcgtag gtagtgagcg cggagctccc
     5641 cacctcgctt tttgccacgg ccacgctgtg gcggacgaag tgagcctgtg cttccagctc
     5701 atcggccacc ggggcgtaag cctcggcata actcgtccta tcgcggtcct gctccagatc
     5761 ctggccggac tgacgaacga gtggcgcgct gtttctgacg gcaacggccg tcaactccac
     5821 gaacttcttc ggaaccgagg cggtagcgat cagccgctgg ccggcccctt tggaagtggg
     5881 aatcacaaga ttcggaatct tctgcacgat ggcgcggatc tcttcgatgc gcgcctgcgc
     5941 agaatcagtg tgggactgct caggtgtaac gggtacttcg gtagacaatg tgttctcctt
     6001 tatctctcat tgcaaactgc tgtgatcaga gcgagcaggt tgaaacgagc ggaggagcac
     6061 ggcctcggaa gatccgtctt gaagcctcga cgacggtcag acaaacagac cgtcgacgag
     6121 caggaatgca gggagcgtcg ttgctttcga acgggaacca attgggtggc cgcagcggcg
     6181 gcggaatcag ccggatggga acgcgcttac ccgcattcgc accctgctcc tcgataggga
     6241 tcgcaatgct gccgcccatc actccgtcga acagccacgt cgaggtgtcc tccagccgct
     6301 tggaaaaagc ggccagttgt ccagcggcct tcatccactg tacggacacc tcggccagct
     6361 ttgccggcgc gtcgagggca agctccggcg actgcgccgc gcggttcctt gtcttacgca
     6421 gaccagccgc tgcgcagtcg agttgtttca aggcccggtc cagccagccc gaggcgatct
     6481 ggatccggcg cgctgcccgg atgggacgct ctgccgccaa actctctgtg gcctcgatga
     6541 cgcggcggca ggcgctgatc aggtcgagcg aagattcgat ggcccgccgc gtgcgccgga
     6601 acggacgagt ccacgacttg cgccggtccc gatccgatcc gcacgagcgc agcccggctc
     6661 gccgcgaatg gttcgcgttc ctttgggagc cggatctgtt ctctgcggtt gccacgtttt
     6721 tttccttcac atctcttaga cgcatgaggt tggcgaaact cgctcgtttt tcgcccaatt
     6781 gtggaaactc agaaaagatt ttacaaagat tttacaaaca taaacaccaa ttttgtcatt
     6841 tcgtcatcga aatcgtcttc gtcgttccca aaatctggct aacgacaacc agattctggt
     6901 catcaacaac caagttccgg tcatcaacaa ccagattctg gctatcaaca gccagattct
     6961 ggtcatcaac agccagattc tggtcatcaa cagccagatt ctggttatca acaaccagat
     7021 tctggttatc aacagccaga ttctggttat gaacaaccag attctggtta tcagcaacca
     7081 gattctggtt aacgactgga gcgaaaggcg ggttacggcg tgatcacaac cttcatcacc
     7141 ccatccgcgc gctcgccgaa gagtttgtaa gctgcgttga tatcggcaag tttgaagcgg
     7201 tgggtgagca ggggagtgag gtcgagtttg ccgcggcgga ccatctcgat gaggcggcgc
     7261 atgcgctctt tgccgcccgg gcagagggtc gtgacgatgc ggtggtcgcc gaggccggcg
     7321 gcgtaggcgt cgtaggggac ttgcagatta tgcgaataga cgccgattga cgacagtgtt
     7381 ccgcccggtc tgagcacccg cagtgcgttc tcgaaggttg cttgcgtgcc gagggcttcg
     7441 atggcgacgt ccacaccgcc gccggagagt ttgcggattt ctgcgatgac gtcggtggtg
     7501 cgatagtcga gcacgatgtc gacgcccatt tttcgcgcgg tggcgattcg attatcatcg
     7561 ccgtcaacac cgatcacgac ggcggcgccc atcagcttgg cgccgagagc ggcgcagaga
     7621 ccgattgggc cctgtgcgaa gacggcgacc gaatcgccga ttttgacgcc gcccgattcc
     7681 gcgccgctga tgccggtgga cgcgatgtcg gtcagaagca ctacctggtc gtcggtcacg
     7741 ccatccggga ttttcgccag gttggcctgc gcgctcggca cgaggagata ctcggcctgc
     7801 gcgccgttga tcgtgttgcc gaaacgccag ccgccgatcg cttcgtatcc gctgccatgt
     7861 ccgcactgcg agaggttgcc ggagaggcag gcattgcact ggccgcacgg tgtgatggcg
     7921 ccgacgatga cgcggtcgcc gattttgtag ccggtcacgc cggcgccgag ctcttcgatc
     7981 acgccgaccg gttcgtgtcc gacgatgagg cccggtttga cgggatactc gccgcgcagg
     8041 atgtgcagat cggtgccgca gatcgtggtc atcgtgacgc ggataacggc ttcgcccacg
     8101 ccggcgcgcg gccgcgggac atcctcgacg cgaatgtcat cgacgccgtg aaacaccgca
     8161 gctcgcatcg tttcgacgtg cgtcgttcgg atgacttctt cttcggccgc aaaggtggtg
     8221 ctcataggag ttctcccctt cactgatgat tgctgcggcc gcggattgcc gccgcatcgc
     8281 acgccacatc ggactgtgcg cacgatcacc agggagctgt gctagttttt cgcgatgtcg
     8341 ttgcggacgt atcaggcagg ctcgcctcga aagccgggcg aagggcttcg gatcggtacc
     8401 gtccgatttc caccgcgcgg cgtgcgcaag gaagactacg cgcggctcga tttcttcgac
     8461 gtctggtttc ccgttgtcgc gccgtcgcgc gagctgctgg cctgggtgaa gaagcacgac
     8521 atcgccaacg atgctgcgtc gatgaggacg tttcagaagc gctacgcgcg cgagttggag
     8581 agcgatcctg cggcgcggta ctcggtgctg ctgcttgcgg cgttggcgca acagacggcg
     8641 atctccatcg gatgcttctg cgaggacgag aacaactgcc accgttcgat cctgaagaca
     8701 gtgattgaga aggcagcggc ggcgctgccg gagcgatgag aggcggcatg ctgcgccgcc
     8761 tctacttcgg tctcgcgctt ctggctctgg ctccgatctg gagcgcgtct tacttgccga
     8821 cctcggatgg tccttcccac ctttacaact cgtgggttct gcagcagctc gtgaggggaa
     8881 gtcatggtcc ggtcgcggat tggtatgcca tcgactggag gccgcttccg aactggctcg
     8941 gacacgcggt gatggcgctg ttgatgatgg tcgtgccgcc gctgatcgct gaaaagctcc
     9001 tcgtcagcgg catcgtgctc ctctttctct acgcgatctg gagatacgcc ggcgcggccg
     9061 atgaatcgcg tcagctgttt gccttcctgg cgtttccgtt cgcgtacaac cttcttctgc
     9121 agcagggctt ttacaacttc tgcatcagcg cggccctcta ctttctcgtg ctcgccgtct
     9181 ggtggaagcg ccgcgacact ccggacgcac gcacgatcgc cgtcgtggct ggcctgctgc
     9241 tcctttgtta cttctcgcat ccgatgtcga cggtcctcgc gatcggatcg atcggcattc
     9301 tctggctggc gacgttgcgt ggccggccgc ttgcgatcca cgcgcggcac ctcgttgcgc
     9361 tgcttccggt tctgccgctg ctggcgtggt tctttcgcgt gcaaggcgtt tcgctggcgc
     9421 gggcgccggt cagcgcggcc ggtctcttca gctacatcat gaggatgtgg gtgctgctca
     9481 cgttcgacga gtttcaagcg aagctcggct tcgcgctctt cctcgctctg gccgggttga
     9541 tcatcattac tctgattcgc cggcgatggc gttggagcga aggagatgcg ttcatcctcg
     9601 tcacgctcgc cctcgttgtc gtctacgcgc gcgctccggc agcatcgtcg ggagggacga
     9661 tgatcatgga gcggatggcg cttttcgtcg tcctttcgcc gatcgcctgg ctggcaccac
     9721 gcctgccgcg acgagccacg atcgcactgg tcatcgtgct ttccgtagtt tccgtcgcat
     9781 acaccggata tctcgttcgg cgctaccgcg gcctctcgcg gcgcgtgacg gagcttgtac
     9841 gcagcgcgga tgcgatcggg cgcgggacgg ctttcgttcc tctgattcgc gaggtccgtc
     9901 cgcacggatc gttcgttgcc gtgcagatgc acgccatcga ttacgcggcg atcgagaagg
     9961 gctccgtcga catcgccaat tacgaagccg ccgccggatt ctttcccatc cgataccgat
    10021 ccggcatcgc gccacccgac acgaacacag ccggcccgag ggcgagcgaa gtcgatctca
    10081 ccgcatacct cgcccgcgtg ccttacgtct tcctctggca tgttcccgat gacgcaccgg
    10141 tgatgtcgca actcgccacg tgttgcgaac gtgtcggcgc gtcaaacggt ggccgcgtgt
    10201 atttgaaaca ttgattgggc ccggcaccgg ggaaagctct tcggtgccga gccggtatgg
    10261 aacgctaccg ctggcccatc ccgcccatcg cgtgcggatg acggaacgcc ttgccggctg
    10321 cctgcagcgc ttcgaatttc gtcttctggt ccggcgtcag ggtggcgacg aattttgctt
    10381 ccgaatcgcc ggccagtgcc cggatggcct ggcgcgccgc gcgaagggac agcgtcagtt
    10441 taccgaccgt ggccggatca ggaggtgtgg ccttcatcgc ggtgtcgagc tggtcgcgga
    10501 ggtcgcgcgc gttctgcacg agcggcttca tcgccgttgc ctgcgcttcc cgcaactgct
    10561 tccaggttgc gagctgatcg ggcgtcggct gcagaacctg cacgagcagg gcgcccatcc
    10621 gtccaccgcg ttgcggtggc gcgcctgcca gttgtgccga agcgattgct gcgacgagca
    10681 gcgcgattgc catgagtgga atccatcgtt tgttcatcgt ttcttccttt cggttgtctc
    10741 tctgcttcaa cgagaagacg gactcgatac gattacgcgt acagcgatcc agcgatgcgt
    10801 gatggccgtc gcatggcgat gtgatgtctc tgatctgtgt acatctgcgt catctgcgga
    10861 caaagggttc atccgcagat gacgcagatg ggacgcagat cagaatcgat cagccgaccg
    10921 cggcggtgac gtgcgccatc gcctgcgtga ggtcggccag cagatcgtcg aggtcttcgc
    10981 atccaaccga gagacgcacg agaccggccg tgattccctg ccgccgttgt tcggactcag
    11041 gcacgctctt gtgcgtcatc gaagcgggat gttcgatcag cgattcgacg ccgccgaccg
    11101 acaccgcgag cgtgatcatg cggacgctgt tcatcaggat gctgcccgcg gtgacgccgc
    11161 cgcgcatttc gaacgcgatg atcgatcccg gtccgtccat ctgctgctgc gcgagcttga
    11221 actgcggatg ggacggcagg cccgggtagc ggacccacgc cacctgatcg tgatcttcga
    11281 gccagcgggc gatggagagg gcgttcgtct gcgcacgctc gacgcgcatg ccgagggtgc
    11341 ggatgccgcg caggaggagc catgattgat gcgggtcgat ggtgaggccg aacatgatcg
    11401 cggcgtcgcg cagcgtcttc agcatcgacg gatcgcgcgc gacgacgacg ccggcgatga
    11461 cgtcgctgtg gccgttcagc gatttggtgg ccgagtgcag aacgacgtcc gcgccgagct
    11521 cgatgggacg ctgcaagtac ggtccggcga aggtgttgtc gacgacgagc ggaatgccgg
    11581 cgccgcgcgc ggtggcggag gcggcgatga ggtcgacgag atcgagcgtc ggattggctg
    11641 gcgtctcgac gtagatgaga cgtgtttcgg gacgaatcgc cgcggtcagc gccgccgcgt
    11701 cggtggccgg cacgaaggtc gacgttacgc cgagtcgcga gaagtagcgc gtgagcaagc
    11761 cgcgggaagc gccgtaaagc ggatcggtgg cgacgacgtg atcgccggct cgcaacaggg
    11821 caagaaagac ggtgctgatg gcagccatgc ctgtggccgt ggcgaccgcg ccgcatccgc
    11881 cttcgagatc ggcgacgcat tcttcgagcg cgtgcacggt gggattcccc atccgcgtgt
    11941 agatcgggcc gtcggaggtg ccgtcaaaac gggcggcgcc ttcttcggcg gtgggaaagg
    12001 cgaaggtcga gctctgatag atcggcaccg ataccgcgcc gcgatgcgcc gccggatcga
    12061 gaccgccatg aattgctttg gtgtgcagac gcttcgcttg gttcgccatg cgaagaatga
    12121 tcgcgtcggg gtggaccggc cgcacatgtc gcgcaacaca cgatcatgtg atgttgcgca
    12181 cgggctcaga aaaccattca ccacggagac actgagatca cagagggttt ctctgtgacc
    12241 tctatgtctc tgtggttgaa ttggttctgc tcagtgcttg gccaggccgc ggatgaaggc
    12301 gaccagcgag ctgacgtcag caacgctcag cttggttttg tacgcgggca ttttgccctt
    12361 gccgtccgag atgatcttct gcaggtcggt atcggactgt ttctgcacgt cggccgagct
    12421 cagatcgcgg atcttcatcg acttgccgat cgaggtggcg cctttgccgt cggggccgtg
    12481 gcaggtcgcg cactttgact tgtaggtgtc ggccgcgccg gggccggcag cgaatgacac
    12541 cgaggcgacg aggagggcga ggatcatgat ggacagtaat tttcgattca tctgtttctc
    12601 cttcaattca gttgagcatc atgctgctgg aatgccaggc agcgcaatct gagcctacaa
    12661 aaaaaagggt ggcggccgaa gccgccacct tcacaaagct atatgacgac gatcactgct
    12721 tggccgggat cgtggtcggg tcgttggttt tcatgtcgat cagagatgcc gaggagagaa
    12781 cgttcgttcc cttcagcacg gtgacggtcg cgaagcccca atcgaggtcc ggcgcgttcg
    12841 cccggttctt gaacggctcg agatcctgca cgccgctgcc aaccggaacg gtcaggttgt
    12901 agctcgtgag cgacttgcca ttcgcatcga acagattgat cgcgacctgc gcttccgttt
    12961 tcccgccgtt ggtgacgacg aggttcgagc ggtaagcgtc cgtcttctgg cggagtccga
    13021 tcaggctgat cgtctggccg acggtgtagc cgaggtcggc gacgcggccg tcgtaactct
    13081 ggccgtatgt gccgttgtcg gcctggttga agatgcgggt ggccacgagc agcggacggt
    13141 cggagcagac ttccagcgcg ccgatgttgc tcggtcccat gatcgcagtc aggtcttcga
    13201 aggccttctg cgaattgccg ttgaccgtgc cggtggcttc gagatcgccg ctggcctggt
    13261 gaaggatgaa acgaagcgac gcggtcgacg actcgaggtt gcgcgccacg agatcggtgc
    13321 gccactggct gccggcctgg cctgccgtgt ggccgaccat ctcgctccag tagacgtagt
    13381 tgctcttgca ggagacggct cctgcaccgt tgccgagtcc gccgccgatc gcgctgttgt
    13441 tcacgctgcc ggggcctggt gttgcggtgg ccgaggcgtt gaacgagctg acggcgaagt
    13501 tcgagatgtc gagtgctgaa ttgacgaagg ccgggttgtg aacgcccatg ctcttgtcgg
    13561 ccagcaccat gaagtagttc caaccagcct tggcgatcga cggatcggcg accgagtaga
    13621 gctcgacggc tgtgccgccc ggacgaacga tcttcaccgt ggccagcgag aggtcactga
    13681 ctttcgttcc gccggtcagc gtaacgttga ctccctggcg gccgtggctg gagatgaatt
    13741 cgacgccgga gatgtcggag gcgttcttga tcgtcttgag gccattgagg tcgatggagt
    13801 ttccggcgcg gatctgggtg accatcaggt tcttgatggc ggtttccatc tgggccttga
    13861 gcgtatccat cttcgcttgc accggcgcct gcacggtgtc tttggtgatg ttggtgtggc
    13921 acttgttgca gatcgtgtcg ctggcggcga acgcgtggtt ggtgccaacg taggagccgt
    13981 cggcattctg gagcgcgagc gcggctggcg gatcggtcga ttccatgtga cacgacacgc
    14041 acgagtcctg aatcatcgag tgaagtccgc gcgtaccgac cttcgtgaag tagaggttct
    14101 ggcccatcac gatgtcggcc tgcggccctt cgtgcggcgc gcgcgtggcg tcggcgacgg
    14161 tgaagtgtga gtcgtcgcga aggtcccggc ggccgttgtg gcaggtcatg cagaccgcgc
    14221 cggtaccgac gctcgttgct ttgaagcctg ccatcagcag gggcgtggtg cccgttacgc
    14281 ggactttggc gttggtgttt ttgtcgctcg aggtggtgcc gacgtcatga ggatcgtggc
    14341 aggccgcaca ggtctgcggt tggacttcgt ccgcggtcca ggtgacgttc agattggcgg
    14401 tgctgaatcc ggtgtcctgc caggctacga atccctgcgc gctatggcat ttcgaacacg
    14461 tgggatccat cccctcggcc actgcggtcg ggaagtcggc gtgcttcgac aattgccact
    14521 gctggtaacg cccgtgacgt gtcggttctc cgtgacagga gccgcatacg tcggacgaga
    14581 gcgacatgcg cgagccgtct ttcttcatgt gggccacgct gtcctgcggt ccatgacagt
    14641 tttcgcactg gatgttcgcc atgcgcgcgg tggcggggta gttggcgaga atcgtcgtcc
    14701 agttcgttgg cgttccgacg gtgagcaagg tggtggcgag gaatgccttc cagtcggacg
    14761 cttcatcgat gccgttgttc tgcaccggtt tggcgttgaa gccgacggtg tggcaggaaa
    14821 gacacgagga ggagtagtga ccggccggcg tggtgacgtt ctgcgtgaag atctcggcgt
    14881 ggcccgactt cgcccacggc gtgaagagat cgaagtgcgg cgtgttcttg acgtggcatc
    14941 ccatgcacgc ggcatcggcc acgggacggc ctgtcgcatc ctgtccggtg atgatgcctt
    15001 tccatgtgcc ggcgtgaacg gtgatggtga ccggcttcgc ggtcgccagg tcgagaatgg
    15061 tgagctgata ggtgccggcg acgtcgggcg tgaactcggg gttctgcgac gtcggatcga
    15121 gcagcgtggc cgtcgatccg gtgggaccgg agagcgacca gttgtactgc gcctgcgtct
    15181 tgccgtgcag cagcaccggc agcagaatgg ggacgttgcg tgtaccggtg gcagtcgacc
    15241 acggcgtgtt gcccgcgacc gttgcggcga agtgataggt acccgaggtc gtcgtcacag
    15301 tgacgtcgaa cttgacggcc gcggaatcgg ccagcgcgtg cggcgatacc gaggcaaccg
    15361 tgaagcggtt ctgaaggccg ccttcgaacg gggtgaccgg gatattggcc ggatactgcg
    15421 agtcagcgat cggcgcctct tccaatgccg tcagcagcgc ctcgcggaag actttcctat
    15481 cgggaagcgc gacggtgacg gtgtcggtgc ctgtgttact gagcgttgcc gccgggccgc
    15541 cgttctgggt ccatttgatg ctgacgagcg acgatccgtc gttgatgacg acgttggctt
    15601 tcgccgtgac cgtcgcaccc ggcacaggat cgccggtgat tgcggtggtg acggtaacgg
    15661 gtgcagcggc ccatgcgccg cccgcgactc ccagaatcag aatccccgcc agcaatacga
    15721 gcagaatgcg gtgatgacga tgtttccctg tctccatgac ctttctcctt ttgttcgttc
    15781 cggaggatgg cgatcacagc gggccatgaa ggcaaacgcc gtcgcgcatc gatggagaca
    15841 ctctggattc gccgcggaca gaaagctgtc cagccggtca ccgtacccac gtacggaaaa
    15901 tcacaggtgt gcatgtcggg agaacgtcac cgacatgggt gatgtcaact tcttcacgcg
    15961 aagcggctct gaggcaacaa agggatgatc ggctaaactg gctccctcag ggtagacacg
    16021 ccaacaagcg ctctcggctc accgctgtct tccatgggca ttgcacacac ctgactcaca
    16081 caaactaagt gcttgtcacc aacgtcgcgc tcggtcacac taagcagatc aatcgctact
    16141 gaagtgtggt ttgggaggct tcatgcagaa aaccgatgcc cccggtgtgg ctttgcctgc
    16201 tgatctgacg gggtggttgc cggctgcgca acgttttatc gaatggctag tcgagttcaa
    16261 gcattggaac acccccgaag cgtggctcgc ccgcggggtc atctgggtcg tagtagttta
    16321 cagcctgctg cgcctgattc gcggcgtact cgaactgctt gtccagttga aaacctattg
    16381 gactggtcta ggttggaagg ttcgacgcct gacagaggaa gagcgcgccc agcttaaccg
    16441 acgacagcga ttcgctaggg tattaagcag tgatctctcc aacatggcga aggtagagaa
    16501 ctggaatgat caattctata ccgacctaga ggcgcagatc gaagccgaag gccggttcta
    16561 cgcgacaaat ttcgaccgca tcatccgacg agcctcggaa ggcttacgaa gagtatcttc
    16621 actggtacga gcgatcgaat ctagcgctga gcagtttttg cttcttatcg gcgagcccgg
    16681 cagcggcaaa agcatcgctc tgcgccatct ggcgcaccaa ctcgccgaat ctgcgctcac
    16741 ttccaaagcc aaaatggtga caattccatt gtatgtcaat ctaaaggagt tgccatcacc
    16801 gacgtccggt gaccccaaca gtgactacat caaggctttc gtccttgaaa acgtgcgccg
    16861 tggcgacgca gataccgcgg aatacatccg tgacaattgg gcaaagcacc gcgacgatgg
    16921 ctcgtggctc ttcctctttg actcattcga cgaaattcca gctgttcttc attctccctc
    16981 cgggagccgc gccgccaccc tttactctga agcaattcgc caattcctct ccacgatggg
    17041 cagttgccga ggcgttgttg cttcacgcga gttcaaaggc ccgcgcaatt tcggatggca
    17101 tcgcttgcga attctggcaa tgaatgagga taaacaggca ttgctcgtgg cgaattcgta
    17161 tctaccatcc gaaaggcgtc ggatggtaat gcaacacatt gcaacttccg aatccacgat
    17221 gcttcagaac ccgatgtatc tcacactcct ctgtcgcttt gtccgtgacg cgaatgcact
    17281 tccacgaaat gaccaggagg tattagagcg tcacatcgtg cgactagccg agcgtgacgc
    17341 cgaccacgtc ggcatgaagt acgcgcttac gaccagacag ctcttgcaag gtgctagcac
    17401 actcgcacgg ctgtttgccg agcaacagga tttgagcctt gctccaacga ttacagatat
    17461 tcggttagcg ttgtcggact cggatgtccc cggcggaagc atcgacaatg taatcggggc
    17521 tctcgtggac gtaaagatcg gacgatctga cgttcctgag gcgagaattg gcgatagacg
    17581 tttcacgttc gctcacagac gatatcaaga aactttgttt gtccaatttc ttgctgcaaa
    17641 tccagattac atcgcgccga aacgtctcct aaccgaaaca cggtggcgtg aatatgcggt
    17701 cactttcctg caaacgcagc cagtcgaaac gctccagtct ctgatccgtg gcgccattga
    17761 cctactaaag gagtacgaca agcggcaatc gtatgttgct gttctgcctg aatacggaag
    17821 caccgccgtt taccatcgtt gggatgatgc gattccgcga ctgctcggtc ttctggagga
    17881 aggtcttgcg ggtcgaccaa atgatgtccc ggcagatctt cgggcggcag ttgctgctgt
    17941 tctaatgcca cgctggcatg acggcgattt tatggatcgt cggaatgtgc ttcgaatcgc
    18001 tggtctcttg ccgccggagt tgctcacaga aatactagtt gaaactgtgc gcggtggctc
    18061 gcatgacact cttgatattg ctctgcgcaa gacggttttt ctgtcgaata ttccgccgga
    18121 attgtcgcga tggattcaaa ggcgcctgag tgatgatact ctcgatgcgg aaaacgatag
    18181 tgagataacc cgcctcgaag cgctggccgc acgtcttccg cagtcggtac gcgccgcgtt
    18241 cattgttagt aactgtaaac ggctgcgccg acgcatgatc ttatgtacaa taccgatgtt
    18301 ttggccgaac gtggcgctgc tggcgctgat caccgtcttg ttggacaagt ccgtacgggc
    18361 tgcgcgacgc cgcgccgagc aagtgatgac agagatggcg gtgcttaacg caattgtcgc
    18421 tgtatctgcg tgcgcggttg tggttgttgg tatttgctgg cgtatgcgcg gcggcgcgac
    18481 gattgccagc gcggtgcact tcacgtcggc gatcgcattg atctatggtc tcgtgggcct
    18541 cgtcgtgtta gcgcagtaca gctctcgcgc ttccggtaaa cacattagcc ctcgagcttg
    18601 ccttaaggcg atggttgggc tgtcgagtga gatgcgaagg cccggtcagg ttgcaaaggg
    18661 ggtagtgttt ctggcgactg gcgtgatgtt cggaatgatg attttggata tctcactcgt
    18721 cggtctatgg ggtagtcgcg gttggctaac ggcactcgta gtgttcgtgg tcatggtggc
    18781 aatagagcct gcgattattg ctattcgccg tcggcggttt cggcaagcgt attcagttta
    18841 tgccgtttca aaacgatgcc cagttgctgt tgcgcttcac gcgacttctc ttcgagcgct
    18901 agttgactgg accaagcaag agacactcct gcgttcggtg cgcgacgtca ggtcgttagg
    18961 tcgaatatta cgctgtgaac cactcactgg cataaaaccg aagtgcaagc atcctatcag
    19021 ggtgcagttt ttgtccgagg agcgcgagtt cgatctccgt gaaattggcg cgagactcaa
    19081 ccaccttgcg gatcgtctta ctaaggccgg tagaccgtag cgcggagttt ttccgcaacg
    19141 gctttcaaag tggacctagg tgggtttatg gccggctagt ggcgagtgtt gcgcccgaga
    19201 tcggaccggc agttttggtt gcattcggtt ggcgagcgtt ccagtccccc cccccccgcg
    19261 gaagccgaac gccaaatagc gccccgctcg gagagcaata gcgtgtcgtc gctccagtgg
    19321 ggtcacacca gggtcacacc ttcgtatgtt tgacgtccga gtcatttgcg agccacggga
    19381 gtcgagtcga gagagcgcct aaggcgcccc ttgtttctcc gaaggcggtc aacgtttcag
    19441 aagagctcct cggccaacgc gacgatgatg cccgcagggc ttcgcatgca gcagagtctg
    19501 tacttgtttt tcgtattggg ccacatcaca actagctagc tcggcgccgt aggcgcgcga
    19561 cagtgtcgtc gacgctttcg accgtaaaca tgacgctccg gaggcccagc gagttcggcg
    19621 gtgagatcgc cgattcgatc tcgatgagct tcggattggt aaacttcgtc agctcgaggc
    19681 ccgtgcccaa cacgatcgcg gctgtcagat cgtcgacggc gacgctggcg tggtccaatc
    19741 tcttgatcgt catgtcgctt gtatagcaga ggccgtgcct ccgcggtcaa tgccggcact
    19801 acaagttaga cttcggcaat gaatgacgta atcaaggttg ccgagtccga cgctgagatc
    19861 gactcctgct ttcctgtcat gtcgctgctt cgtccgcatg tgcagcgtgc ggagtttgtg
    19921 cggctcgtcc gaaagatgga gacgcaggga tatcgtcttg cctacctgac cgcggacgat
    19981 cgcattcagg cagtggccgg gtatcgcatc atcgacatgc tgcgcacggg tccgatgctc
    20041 gaagtggatg atctgatctc cgccgccgac gcgcgatcga aaggatacgg aaagcaaccg
    20101 ctcgattggt gctacgacct ggcccgccgc agcagctgca gcgtcgtgga gcttgattcc
    20161 gcggtacacc gcgccgatgc ccaccggttc tatttccggg aacggatgca cgtgctcgcc
    20221 tttcacttca gccggacggt ggtgtgatgt tctccacgtc tccgcgtgaa atctgaaagt
    20281 gtgatttcac gcggagcgcg agagacgact cacttcgact tcggccgcac ggacatgcgc
    20341 tcgagcggct gcagcatgct ggcgcggttg ccttcggtgt cgaagaaggc cacgtatttg
    20401 ccgacgccgg gaatgtccat tggatcgccg aggactttgc cgccggcgtc ggagacggtc
    20461 ttcattgatt gctggatgtc gtcgaccgca atcacgaccg ctggatactg cgcgggcctt
    20521 tcgctgctct tcggatagaa gccgccgttg atggcgccgg ctttcttcgg gccgttgtcg
    20581 tcggattcgg tcgtcgtcac gacaacgtag tcgcccatgt cggggccgag caattgcgcc
    20641 tgccagccga acgctttctg gtagaaattg gccattcgtt tgcggtcttc ggcgggcatt
    20701 tcgaagtgga ctacgggatt catggtcatc gaacacctcc tcgtttcgtg ggatgcagtg
    20761 atcgttccta tcgtgccggt cggttcggct tttttcgcat gagaatggtt cacgccgaga
    20821 cgcggaggac gcggcgttac tatggttttg atgaaacgga tcgcgctgct ttcgctgctt
    20881 atgttcgcct gccgcgatac gcaccagcac ggtgtgacga tcgcgcggag cgggaaggct
    20941 gcggcgcgga tcgtcgtgct gacgatcggt gtggccatca aggagacggg gccggcagag
    21001 acggtgaaga agttcggcga ggtgtacgcg ttctcacccg aggtgtttgc ggtgcggcgc
    21061 gatcaaccga cggagatcgt gttctggaac ctgcagccgg acgacgagca cgacttcatg
    21121 ctcaccgatc cggcgaacaa ggtcctgttg cagacgaagt ttccgccgct atcgaagacg
    21181 tccttcgtga tgacgtttca cgatgcgggc gtttttccgt tctactgcac gatgcatcag
    21241 ccggcgatgt cggggcaggt tattgtttcg ccgtaaacct gtttcacgcg gagacgcggg
    21301 ggacgcggag gagaattgcg ggcaacaatc gcagtaacga ttgcgctgat cgcgcatacg
    21361 ggtttcgccg gtacggtcaa acgttgcggc gtttcgttcg ttctgccggc aaagtggacc
    21421 gctgtcgaaa aggtgtcgcc cgactatccc gatgagacgg acatccaatg ccgcgtcgcg
    21481 ttgcggcccg agggctggag cgcacgagcg aaaaggtcgc gatgggacgc gagcgatcca
    21541 cccgtgtggc ttatgatctt tggtgcaggc acgtcgttcg aggatgcgct cgatggggcc
    21601 ggattcgaga acgaattcga ggaaggaggc ggcaggctcg gcatccccgg ccggttcgga
    21661 ttctcggaag cggagcctta ctcagccggc cgcttctccg gactcgtcgt gcgtccgacc
    21721 ttccgcgggt tcatccgcga cgtgtcgacg ctgcgcgaaa acgagtcggg cctctccacg
    21781 cgcgacaacg aatcagtcgt gatgaaagag ccctccgggc gcatcatcgg gttcgcgtct
    21841 gacggaggaa cgcctgatga accgatcgac cgcgatgcga tcatcgccgg catcgcgcag
    21901 acgctggttt tcgaagcgcc gaaaagcaat gaaccacaga gacactgaga tcgcagagtg
    21961 acctccgtga cctctgcgtc tctgtggtaa ccgcttctac gaaactgccg gcgcagccgc
    22021 cggcctccgg ctccagtacg acaccgcgag gagaaagtcg gcgacgttgg ttgaggcgaa
    22081 ggtgggggcg agatccgacg gggcgacgat tccgacgaca tggccggcgt cgtcgacgac
    22141 cggcaggcgg cggacctgcc tggcttccat caactggatc gctgcgtcga cttcctcatc
    22201 ctgatggatc gtaaagacgg tcttcgtcat cacgtcgcgg accgcaacgg cggcggggta
    22261 catgcctttc gccacggcgc ggcaggtgac gtcgcgatcg gtaatcacac cgacgagctt
    22321 cgaatctttg cagacaggga cgatgccgca gtcgtgatcg agcatcagtg tggcgacctg
    22381 atcgatcttc gtatcgggag tgcagcagac gggagcgggt gtcatgactt ctttgaccaa
    22441 catgatcggt acctccattc gggacctgtc atgtgaatac gtcctgtcgt cgccgcgaac
    22501 aaggagaggc gtcactcctc tgttgtatga gtgaacactg agttgagtgt ctgcgcgctg
    22561 agcacgtgtt cttcggcacg ccctcccaat cgagcgtcct tctcccccac gctttttgtg
    22621 ggggagaagg tgccgaaggc ggatgagggg gctgttctaa gcagagaagt tacgcagtcg
    22681 tcaaaacaag ctcttcgttc acttcgctca gctcgcgaac cttgcttcca acgtacaact
    22741 ggcgcgggcg gttgatcctg gtcttgtcgt cgtcgaccat ttctttccac tgcgcgatcc
    22801 agcccggcag gcggccgagg gcgaacatga cggtgaacat gttcgtgggg atgcccatgg
    22861 cgcggtagag gatgccgctg tagaagtcga cgttgggata gagcttgcgg tcgatgaagt
    22921 acggatccga cagcgcgatc tgctcgagct ccgtcgccgt ctgcaggaga ggatcgcgtt
    22981 tgccgagggc ggagagaacg tcgtcggcgg cgcgtttgag gatcgtggcc cgcggatcga
    23041 agttcttgta gacgcggtgg ccgaatccca tcagcttgaa gccggagtcc ttctgcttgg
    23101 cgcgctccac gaacgtcctg gcgctctggc cggactcgcg gatctgctgc agcatctcga
    23161 tgacctgctg gttggcgccg ccgtgcagcg gtccccacaa cgcgcagatt ccggcggaga
    23221 tcgacgcgaa gagattcgcg ccactgctgc cgaccatgcg cacggtggat gtgctgcaat
    23281 tctgctcgtg gtcggcgtgc aggatcagca gcaggttgag cgcgcggtcg atggccgcgg
    23341 tcaccttgta gccttccgcc ggcaccgcga acatcatgtg catgaagttc tgcgtgtagc
    23401 tgaggtcatt gcgcgggtag atgaaaggct ggccgatcga cttcttgtac gagaacgcgg
    23461 cgatcgtctt cgctttcgag aggaggcgga tgatgttcag ttcattgtcg ttttccgggt
    23521 agtaggcgga gagcgacgcg atcatcgccg agagaatcgc catcggatgc gcggacggcg
    23581 ggaagccttc gaagaacttc ttcatgtctt cgtggatgag gctgtgccgc gtcagctttt
    23641 cattgaacgc gtccagctcc tggggagacg gaagcgtccc gtgaatcagc agatagcaga
    23701 cttcgacgaa ggtggctttg gcggccagct cttcgatcgg atagccgcga tagcgaagaa
    23761 tgcccttctc gccatcgatg aacgtgatcg cgctggcgca cgaaccggta ttgccatagc
    23821 ccggatcgaa cgtgaccgcg cccgtcttcg tgcggagtga ggtgatgtcg atcgcggtct
    23881 caccttccga tccttccatc accgggagct cgtattcttt tcccttcagc aacagcgtgg
    23941 cggtgctcat gaacctctct cctttcgatt gttggtggtt acaacttcgc cgttccggca
    24001 aggccggcat tcctggcagt ttctgcgacc gtcgccgcga cggcctgatg aacgttccga
    24061 tcgagacacg acggcacgac gtcgccgggc ttggctagcg acgcaatcgt ttccgcggcg
    24121 gcgatcagca tctcggtggt aatcgcgcag cttcgcgctt cgagcgctcc gcggaagagc
    24181 cccgggaacg ccagcgcgtt gttgatcgat cttccatccc ccgcgaacgc cgcacccgcc
    24241 gccctcgcct cggatggcag gatctccggc ttcggattcg tcagcgcgaa gatgacttgt
    24301 ccgttgtgaa tgtcacttgc gtcgatgagg cctggccgtc cggtggcggc gatgacgatg
    24361 tcggcggtgg ccatgatcgt ggcggcatcg gaagcgatgg ctcctgcgcg ctgcacgcgg
    24421 ctgacggcgt cagggttaat gtcgctgacg agcacttcgc cgacgccgta cgaaacggcg
    24481 agcatggcga tggcgctgcc ggctgccccg agtccgatca tgccgacgcg cgagcgtttc
    24541 agatcgcgcc cggtgatctt gcaggcgttg atgaccgcgg ccaacgtgac ggtggcagtg
    24601 ccgtgctgat cgtcgtgcat caccggtttg cagagtcgcc ggcgcagctc atcttcgatg
    24661 acgtagcaat cggggatgcg gatgtcctcg aggtgaatgc cgccgaacgt gggggagacg
    24721 cgcatcacgg tctcgacgaa ctcgagggga tcgagcgtgt cgatcaggat cggcgtcgcg
    24781 ctgatgccta cgagctcgtc gtagagcacg gccttccctt ccatcaccgg catgcttgcc
    24841 agcgggccga tgttgccgag cccgaggacg cgggaaccgt tggtgaagat gccgacggag
    24901 ttgccgaccg acgtcagatc ccaggcccgg ttcggatcct tgacgatggc ttgcgacacg
    24961 cgggcgacac ccggcgtgta gatgctgcgc atgtcggcga gctggtcgag atgcacgcgg
    25021 ctggtggagt ggagcttgcc gccgcgatgc gcttcgaaga cgcggtcggt ggactcgacg
    25081 acatcgacac cgtcgatctc gccgatgcag cggacgacat gaatcgtctg cgcttcgtcg
    25141 atggtctcga tggtgacgtc gcgcagagtg gcggtctcgc cgacctggat cgtggagacg
    25201 tcgccgatca atccccccgc ttcggcgatg gtgttggcga ggcgcgcgag ctgaccggca
    25261 cggtgctcga cacgcacgcg caggacagtg tcgatgacgt gttcgctcat gaaacgcgaa
    25321 tctgcccgaa gcgaaggcga atgcccatcg cgtacgtcac actcctgcgc gttctacgtc
    25381 acatgggagg ggtaagaccc gaagttggaa gtggaaccac agagacacag aggacacaga
    25441 gaaaaacact ctgtgctctc tgtgtctctg tggtgaagtc tcgtcacttt cggtttcgat
    25501 cgcaaaaaga ccctttcgca aaaaaaagac gcggcagcaa acgccgccgc gtcttctccg
    25561 gaactgccgc agcgactcat gcggctttgg tgaggagctt cttcggttcg accttgatgg
    25621 tcttcggttt cgctccttcc ttcttcggta gctgcacctc gagcagtccg ttggcgaacg
    25681 tcgccgcggc cttctcggca tcgatgttca ccggcagcgt gaaggaacgg aagaacgtgc
    25741 cgtagtcgcg ctcgatgcgg cgatagcctt tctcttccag ttccttttcc gcggtccgtt
    25801 cacctttgat ggtgagaaca ccgttctcga tctggatgtc gacatccttc tcgttcatgc
    25861 cgggaagctc cgcgcgaagc acgatgtcat tcgtggtttc gatgacgtcg gtcttcggcg
    25921 cccaatccgc cagcggcgtc ttgtcctggg tgagctgcgc ccagagacga tccgtccgtc
    25981 cgcgaagcgt ctgcagctcc tcgaagggat cccatcgctc aaacctggtg aggaaattca
    26041 tggtgtacct cctgtttttc attgccgcgt gctgcggcaa tcagaagata ggtccgtgat
    26101 gggattgcgc aattgtcagc ggtcaccaat cgatacgtac gcttgtctcg gtactgtgtg
    26161 attgcggcta aacgagagcg cagaggttct ctccgcgccc tccgcgtctc cgcgtgaaac
    26221 gcttttcctg caccagagga gactgaggtt caggccagat ggtgtttcag cacgtcatct
    26281 gtattccgtg agaaatagat gaccaccacg gagccaaaga gaatcgccca cttccatggc
    26341 tggacctcgg ggaggtcttg cgaaatgtag atctggatgc cgtaaatcac cggcaacgcg
    26401 atcaacagaa tccaactgca ccagaagagt gttttgcgca tgacgggtct ccttacgtcc
    26461 cgatcttgcg tcccgaaatc tcctcggggc gatgcgacgc gttacgctcg gaattgacgc
    26521 acgtcacgcg ttgctgtgac gttgcggcgg cgcagcgatc gacggccgtc ccgctccagg
    26581 cggacgagct ccgaagagcg tgaagagatg gaccgcggag agcgcgcaga cgaggaggag
    26641 gatggccagc acccatccgc ccgattgcgc gatccagtcg cggccaccgc gggcgatgtt
    26701 gagcgcttca tcgatctcgc ctccgcgata cgtcgtgccg gccaggtact tcgccgtttt
    26761 cagatccggc gtcagtgcga ctacgacgtt agggtgagcc aggagtccat tcgcatcgcc
    26821 gacgcggtat ccgatcgcgt cgagcaatcg ccgcgtatcc gccggcttcg ccatcaccac
    26881 cgtccaggcg agggggaggt tgtggcgtac gcgaaatcgg tgcagatcag cgggtgtatc
    26941 gcgcgcatcg aacgagaaga tgacgacgcg ataggtcgac ggatctgccg atgattcggc
    27001 ggtgccgctc ttgagaccct gaacaatgag ggggcaagca atcggacacc gcgcatacat
    27061 cggcgcgagg atcgtcggcg taccgcgcca ctcggcggtc gagcgcgtgc ggccggtgtc
    27121 gtcgatcagg tcgatcgtcg gaatcgttgc gccgacggga aactcttgcg cagcgctcac
    27181 acggccgccc gcgcgtagcg tgacggccgc accagcgagg agaagagcga ttacgaacga
    27241 acgtctcatt ttcgcagtgc gacaggactg gcaggggagt accgcggcgc accttcgtag
    27301 cgcgctttcg cgagctcgac gaaaggcatc gtgtacgcga cgatgagcag gatgactgtt
    27361 gccacgagcc acggccggag actctgcagc gccacgatgt cctcgtcgtg atagggctca
    27421 gtcaccggca gctcgagcac gcctgccgac gatttctttc gcaacagcgt gccgaagaag
    27481 atgacgaagt agagcagcac cgtcagaaac atgatcacgc cgccgatcgt gccggccact
    27541 ctcgccacat tccacgatgg ttgataaagc ggcgaagcgg gattcgtgta cgacaggccc
    27601 atgttcgtcc ggcgcggctc gcctgcgatg ccgccgatga acaggccggt cgagaagacc
    27661 atgatgccga gggtccagag atacggaatc cagacgttca gccgtttgag cgcgatctct
    27721 tttccgccga gcgtcgagat cagcaggagg cttccgccga tgaatgccag aaacacaggc
    27781 cctgcaaccg tctgatgaaa gtgtgccggc atccaggccg tgttgtgcac gacgttgtcg
    27841 agactgtacg acgcgttgat gatgccggtg atgccgccga agatgaagat gaacaggccg
    27901 cagaagagat aggcgaacat ccagcgatcg gtatcgagcc acggcagctt cttccaccag
    27961 ccgaacagcc ccgtgccgcc gctcttccgc gcaccatgct ccatcgacgc agccagcgtg
    28021 aatgccgtga cgaggctagg aatcgccacc agcatcgtga agaacgcgtg gacgaacttc
    28081 cacgtcgtgc tgatgcccgg ctccgtgtat tgatgatgaa tgccgatcgg tgctgagaag
    28141 gcgatcagcc acatgaaggt gaagcgcccc gcgaaatcgg agtagagctt cccgccggtc
    28201 agcttcggca gcatcgtgta gtacatgacg tacgccggca acagccagaa gtagacgagc
    28261 gggtggccga agaaccagaa cagcatgcgc gccagcggca cgttcacggt ctgcgtccag
    28321 ccgagggacc aggggatgag gaggacgatg atctcgatcg cgacggggat ggtggcgatg
    28381 aaccagacga tgaacgttgc gaagacgccc agcactgcca gcggcatctt cgtcgccgga
    28441 ttctcgcgct tccacttctg aaaaacggga atccaactga agaaggcgac ccatgatccg
    28501 atcacgagaa ggacgagccc aatgtagaac gcgggatggg ctttcagcgg cggatagaac
    28561 gtgtagagca cgctggcgct gccggtcagc atcggaatcg cggccatgat cgtgcccccg
    28621 gtcatcagaa tcagcgacca ccaggcggcg ccgaggagga gcggcttgcc ggtgtagaag
    28681 cgaatgaccg cgtggccgaa tgcaaccgcg aagaacgtcg tgaagacgat ggcgttgatc
    28741 gcgccgtgca gcgtcagccc ctggtaatag ttgatgccga ggaagctctg tgacttgatg
    28801 ataccggcgc gaaagagcac ctgcagaaga cccatcgtga tccccaggat gagaaggatc
    28861 atcggaaaca cgatctcgaa aaggatgatc ttcgtcaggc ctggctcgac atccgttcgc
    28921 gaatacggct gcaccgctga tttcattttg ctcctcggtc agctacttga ctgtcttgat
    28981 gaattgaatg atcgcgcgca tctccgcggg agggatcgtg gtctccggca tcagcggatc
    29041 gaagcccttg accgtcttcg cgttcggttt ctcgatcgat tcgagcaggt aggcgtcatc
    29101 agcaacggcc gtcgtgccgt cggccaacgt gcgtttgctc ccgtagaggc ccttgaacgt
    29161 gggtccgggc atcgggctgc catcgagtga gtgacacgcg gtgcaggcgt atttgtcgag
    29221 aagctcctgt ccgcccaatg ccgcctcggc gacgggtggc ggaggcggcg cggctgcacg
    29281 atcggtgaca tggattcgcg ccgccatctg gtgatgctcg attccgcagt actcattgca
    29341 gacgacgaga tactcgcccg gctgatcgaa cttcacacgc gcgtaattca cggcgccggg
    29401 gacggccatc aggttgatgt ttgtgccgat gatctgcatg ccgtgcgtca cgtcggcggt
    29461 gctcagataa atgtccgcag tcgatcccgg cggaatcgaa atatcggccg gctcaaatcg
    29521 ccacatacgg gcgagatagt gaacctcata acgcttcggc gcctgcgtaa tgactttgcc
    29581 atcgcgaaaa gggcgcacat cggtcagaca ggtaggcaat ccgaaaccga agaccttgac
    29641 cgacatggcg atgacgccgg tgagcgtgag gacaaacagc gccgcggtga cgacgatgcg
    29701 cttttcgatg ccgatggtcg ctgtggtcat cgctcagcct cgcgacacca tgacaaacat
    29761 gagcagcaac cagaacgcgg cgtagaacac gaccatcgcg gcaaagaacg ccactgcgcc
    29821 gcgcgggaag aagggagtct cgtcgtccat aggtggttta cctcttttgc gggggtcgcg
    29881 ggtcgggagt cgggggtcgg agcggcagtg tctacgacac ccgacccccg accggcgacc
    29941 cccgcattca acaagggctc gctacacgac gcggtgccgc ccgtcacatc agaatgtgat
    30001 ctcgcgagtt gcgcacacat cgctctgtgc ggattcgcac aatgactcgt acgccagcgc
    30061 acatcagcaa gcgagcggga cgcctacatt ccaccgcgtg ctcacagagt gccgtgagcc
    30121 cgaaggaggg cgcattgagc ttgccaccta ccatcgcgca acaaatgaag cggtccggaa
    30181 ccagccggcg tgacttcatg accctttgcg gcaaattgat ggttgccgcg ccattcggat
    30241 tagcgctgac gaatttcacg agtcccaacg cagtcgcggc cgtgatcggg cgagccagac
    30301 ggccctccgt catctggttg cacatgcagg actgcaccgg atgcacggaa acgctcctgc
    30361 gcacctcgga gcccgcactc gacaccctcc tcttcgacat catctcgctc gactatcacg
    30421 agaccgtgat ggcagcctcg ggcgcggatg ccgaagcagc gctgcacgac gcgatgacgc
    30481 agaacgacgg caagtacgtg ctcgtggtcg agggatcgat tccgaccaaa gagaacggca
    30541 actacctgaa gatcgccggg cgcaatggca tcgacatgct gaaggatgtg gccagccact
    30601 ccgcagcggt catcgccatc ggctcctgct cttcgtgggg cggtgtgccg tcgagcggcc
    30661 cgaatcccac cggcgcgacc ggcgtcgact cgatcatcaa ggacaagccg gtcatcaacc
    30721 ttccgggctg tccgcccaat ccgtacgtct tcctggctac agtgctcgag tatgcaacgg
    30781 cggggaaagt gccgaaactc gattcccagg gacggccgaa attcgcatac gaccgcctca
    30841 ttcacgacca ttgtccgcgc cgcgcccact tcgataatgg ccgcttcgcc gcgcaattcg
    30901 gcgacgccgg gcatattcag ggctattgcc tctacaaact cggctgcaaa ggtccggtca
    30961 cgcacgcgcc gtgctcgacc cgccatttca acgaagtcgt ggattgctgg ccgatcggca
    31021 tcggcgctcc ctgcgtcggc tgcacggaaa aggacatcgc cttcaacgtt cccattttcc
    31081 agactgcgga catgaagggc caggcgccac ctgccttcta cccggaggtt tcgcccgagc
    31141 acaaaggcat gagcccgatg gcgaccggcg tcgccggact cgttggcggt gccctggtcg
    31201 gtgccggata catcgcgtca cggaaactcg acaacaccag cggcgaacag ccgaaggagt
    31261 aagcgatggg cacggaccgg cg
//
LOCUS       DATLVW010000884         5537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_36564, whole genome shotgun sequence.
ACCESSION   DATLVW010000884 DATLVW010000000
VERSION     DATLVW010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36564"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VNN25_26200"
     CDS             complement(<1..253)
                     /locus_tag="VNN25_26200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95093.1"
                     /translation="MAELGGGEGEGSFQPGKDLNQIAKDGRAEAFFVEKLTAVMTEGA
                     DAPVVLPLCLKFDGIENETGVARDFKRVALEIRRVINEHW"
     gene            398..2701
                     /locus_tag="VNN25_26205"
     CDS             398..2701
                     /locus_tag="VNN25_26205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95094.1"
                     /translation="MKNWRGAERVFDWSFYSQGTNDQRNASADVFIANALEAFGDPDP
                     KVGSAWERGARLASLVGKVRCLLVLDGLEPLQYPPGPMHGKLKDAAIEALLKGLAAQN
                     NGLCVVTTREKIPDIQQYYGRTADDFELTALTDLAGAALLHDQGATRAGAREISRDDK
                     ELQAASREVHGHGLTLQLLGQYLRLVEDGDILKRDTVRLADADREYQNDATRPYGHAF
                     KAMEAYEKWFEREGTQGQRQLAVLRLLGLFDRPASKSCLDALRKRPAIAGLTAPLVAL
                     QSRDLTAIKHHLEEIQLLTVQADGSLDAHPLLREYFGQRLRDRQPEAWRAAHRRLYEH
                     LYATTPDKPDPTLEDLQPLYQAVAHGCHAGLQQEACEKVYDDRIVRRDEFYSTKKLGA
                     FGSDLGAVACFFDTPWSRVSTALPEDYQAWLLNQAALRLRALGRLSEALEPMRAALEM
                     GIKQESWTSAAVRASNLSGLELTLGEVAGAVRDAEQSVTYTDRSGEASQRMINRTTHA
                     DALHQAGRRDEAEARFREAELMQAEWQPGYPLLYSVWGFRYCDLLLSEGEREAGKAER
                     ELKKEEGLALCRAVAERAAQTLEWAEQNNVDLLDIAVNHLTLGRAALYAAILESRSRQ
                     REPVQPSSPTTSESHRGFTRAATELDRAVSGLRRAGQQDDLPRGLLTRAWLRSLTGAR
                     TGPESAQSDLDEAWEIAERGPMPLYLADIHLYRARLFFRDANYPWQSPQHDLAEARRL
                     IFKHGYLRRKEELEDAEAALEQFEKVH"
     gene            3145..4023
                     /locus_tag="VNN25_26210"
     CDS             3145..4023
                     /locus_tag="VNN25_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXH95095.1"
                     /translation="MSVTDTTSHVPTVTLNNGLRMPLLGFGVFQIPNPEECERSVTDA
                     LRAGYRLLDTAASYGNEEAVGNAIKRSGVPRDELFVTTKLWLQDAGYDKARRAFDRSM
                     QRLQLDTLDLYLIHQPIGDVYGSWRAMEELYREGRIRAIGVANFHPDRLMDLILHNEI
                     APAVNQIETHPFHQQIEAQTFLQENGVQMESWGPFAEGKNNIFRNELLRAIGEKHGKS
                     IAQVVLRWLTQRGVVAIPKSVHKERMAENFDIFDFELKPDEMSSIATLDTGTSSFFDH
                     RDPAMVKWLSEAKRPT"
     gene            4128..>5537
                     /locus_tag="VNN25_26215"
     CDS             4128..>5537
                     /locus_tag="VNN25_26215"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH95096.1"
                     /translation="MAGLSSITNIVVLMFENRSFDNIFGNLYLNGSTVNGYSFEGVTA
                     GTEPNDSKPWTDVQPVLWNDEMVTTPNPDPGEIFQDVNMQLFGSTQDGPPWPSASSLG
                     TPPMNAFVNNYTLQSDPNGPLSSEVPRKRSWGPPGYAGAGTDAVGSDIMHCFNYGTLP
                     VSTLLATQYAVSDQWFASVPTQTYPNRMYAHCATTWSRDLVHHYKEYYDDWDMVLLHS
                     VTNSIFQELDAQGAAGNLRNATAPYWKVYYPRKAGSFSISEALLHYVSGSSQVVDISE
                     LAADALAGTLPLYSFIEPPYGMPNPHISKIPIDSSYHPPYDVLQGEQFLQQVYLTLFD
                     PSNNPNWETTLLIVTFDEHGGTYDHVPPPPAPLPNAAAYRLDGKDVPANPSAPPFDRY
                     GVRVPFFVVSRFARRNYVSHVVADHTSITRFIENRFDLGAMTKRDANAWPLLDLFDFE
                     HPPFDDPPTEEVLAEPSPAG"
BASE COUNT         1073 a         1859 c         1678 g          927 t
ORIGIN      
        1 cccagtgttc gtttattacc ctacggattt cgagcgcgac acgcttgaag tccctcgcga
       61 cccccgtttc gttttcgatc ccatcgaact tcaagcacag agggagcact accggcgcat
      121 cagcgccctc ggtcatgacc gcggtcagtt tctcaacgaa gaacgcctca gctcggccgt
      181 ccttcgcgat ctggttgaga tccttccccg gctggaaact accttcgcct tctccgccac
      241 caagctccgc cacacagccg agcgcctcat cggccgcgac gaagatctcg cccggctaga
      301 cagcgcttgg aagggcaaca agaacgtcgt catcgtccgc gccttcgggg gcatgggaaa
      361 gacttcgctc gtcgcgactt ggatggccga gttggcgatg aagaactggc gcggcgccga
      421 gcgcgtcttc gactggtcct tctacagcca gggcaccaac gaccagcgca acgcctcggc
      481 cgacgtcttc atcgccaacg ccctcgaggc ctttggcgat cccgatccca aggtcggctc
      541 cgcgtgggaa cgcggtgcgc gcctggccag tctcgtgggc aaagtccgct gcctcctcgt
      601 gctcgatggc ctcgagccgc tccagtatcc gcccggcccg atgcacggga agctcaaaga
      661 cgccgccatc gaggccctac tcaaagggct cgctgcgcag aacaacgggc tttgcgtcgt
      721 cacaacgcgg gagaaaatcc ccgacatcca gcaatactac gggcggaccg ccgacgattt
      781 cgaactcacc gcgctgaccg acctcgccgg cgcggcgctg ttacacgacc agggagctac
      841 gcgtgccgga gcccgcgaga tctcacgcga tgacaaggaa cttcaggcgg ccagcaggga
      901 ggtgcacggc cacggcctga cgctgcaact gctcgggcaa tatctgaggc tcgtggaaga
      961 cggcgacatt ctgaagcgcg acacggtgcg actcgctgat gccgaccgcg aatatcagaa
     1021 cgacgccacg cgtccgtatg gccatgcctt caaggccatg gaggcctacg agaaatggtt
     1081 cgagcgcgag ggcacgcaag gccagcgtca gctcgcagtc ctgcgtctcc tcggcctttt
     1141 cgatcgaccg gcctccaaga gctgcctcga cgctctgcga aaaaggccgg ccatcgccgg
     1201 tctgaccgcg ccgctcgtcg cgctgcagtc tcgcgacctg acagccatca agcaccactt
     1261 agaggagatc cagctgctca ccgtgcaagc cgacggttcc ctcgatgccc atcctctcct
     1321 gcgcgagtac ttcggccaac gcctccgcga caggcagccc gaggcgtggc gggcggcaca
     1381 tcggcggctc tacgagcatc tgtatgcgac tacaccggat aagcccgacc ccacgctcga
     1441 agacctccaa ccgctttacc aggccgtggc ccacggctgc catgctgggt tgcagcagga
     1501 agcatgtgag aaggtttacg acgaccgcat cgtgcgaagg gacgagttct acagcacgaa
     1561 aaaactcggc gcgttcggtt ccgacctggg tgccgtggcc tgcttcttcg acactccgtg
     1621 gagccgcgtc tcgacagcgc tccccgaaga ctatcaggct tggctgctga accaagcggc
     1681 cctccgcctg cgcgccttgg gccggctgtc cgaggccctc gagccgatgc gagccgcgct
     1741 ggagatgggc atcaagcagg agagctggac aagcgctgct gtccgtgcct ccaacctgag
     1801 cgggctggag ctgacgctgg gcgaggtggc cggggcggtg agggacgccg agcagtccgt
     1861 gacctacacc gaccgaagcg gcgaggcgtc tcagcggatg atcaatcgca cgacccatgc
     1921 cgacgccctg caccaggcgg gccgccggga cgaggcagag gcgcgcttcc gcgaggccga
     1981 gctgatgcag gccgagtggc agcccggcta cccgctgctg tattcggttt ggggcttccg
     2041 gtattgcgac ctgctcctat cggaggggga gcgagaggcg gggaaggctg aaagagaact
     2101 gaagaaggaa gaaggtcttg ccctatgccg cgctgtcgcc gagcgcgcgg cgcagacgct
     2161 cgagtgggcc gagcaaaaca atgtcgacct cctcgacatc gccgtcaacc acctaacctt
     2221 gggccgcgcc gcgctctatg cggcgattct cgaatcgcgg agccgccaac gtgagccggt
     2281 gcaaccctcc tccccgacca cttccgaaag tcaccgcgga ttcaccagag cggcgacgga
     2341 gctggaccgc gccgtgtccg gcctccgccg cgccggccag caggacgacc tcccgcgcgg
     2401 cctcctcacc cgcgcatggc tgcgcagtct caccggcgcg cgcacgggac ccgagagcgc
     2461 tcagagtgac ctggacgaag cgtgggagat cgccgagcgt gggccaatgc cgctgtacct
     2521 ggccgacatc catctgtatc gcgcgcggct cttctttcgc gacgcgaact acccgtggca
     2581 atccccgcag cacgacctcg ccgaggcccg tcgcctgatt ttcaaacacg gctacctgcg
     2641 ccggaaggaa gaactcgaag acgccgaggc ggcgttggag caattcgaga aagtccactg
     2701 aatgttcgcc gggcttgttc ttagatcggg acccgtttca aaccatcgga ggtatcaggc
     2761 cggatccaaa ccaccgaggc gggacgcctc gatggccgcg tgcgagacgc ccgcactccg
     2821 gcgtagggag tgcggtcgtc tcgaccgcgg gcactcgggc gtcctcgccc gagggcggtc
     2881 ggggcggagg tcgaaactcg tcgacgttct tgctcccgaa gcacgggccc ggccgagcct
     2941 cgtccgcgtc aacttgctgc cctcccacgc ctcgcgaatc gtctgcacaa cccgctctac
     3001 cttttcgacc cctttcgcaa tccggcacgc ccccccgccc acggtggccc ggcgcctgca
     3061 cgtcgatctt tcccgcagct tcatcgacct ggtgtgggac ggcacgatcc atcccggaaa
     3121 gggttcgatg gaaggagccc agcaatgtcc gtcaccgata caacgtcaca tgttccgaca
     3181 gtcacgctca acaatggtct ccggatgccg cttctcggat tcggcgtttt tcagatcccg
     3241 aatccggagg agtgcgagcg gagcgtcacc gacgcgctgc gtgccggata ccgccttctc
     3301 gacacggcgg cctcgtacgg gaacgaagag gcggtcggca acgcgatcaa gcgcagcggc
     3361 gttccgcgcg atgagctctt cgtgaccacg aaactgtggc tccaggacgc cggctacgat
     3421 aaggcgcgcc gggcattcga tcgatcgatg cagcgtctgc aactcgacac actcgatctc
     3481 tatttgattc atcagcccat cggcgacgtg tatgggtcgt ggcgcgcgat ggaagagctg
     3541 taccgcgagg gccgcattcg cgcgatcggg gtagccaact ttcatcccga tcgcctgatg
     3601 gatctgatcc tgcacaacga gatcgcgccc gccgtgaatc agatcgagac gcatcccttc
     3661 catcagcaga tcgaggcgca gacgtttctg caggaaaacg gtgtgcagat ggagtcgtgg
     3721 gggccgttcg ccgaggggaa gaacaacatc ttccggaacg agctgctccg cgccatcgga
     3781 gagaagcacg ggaaaagcat cgcccaagtc gtactgcgct ggctgacgca gcgcggtgtc
     3841 gtcgccattc cgaagtcggt tcacaaggag cgtatggccg agaacttcga cattttcgac
     3901 ttcgagctca aacctgacga gatgagctcc atcgcaacgc tcgacaccgg aacgagcagc
     3961 ttcttcgatc accgcgatcc ggcaatggtg aagtggctga gcgaagcgaa acgtccgacg
     4021 taactcgaac gagccagcgt tgccgaggcg ccaataaaca aaaaccgtct ggtctgccga
     4081 ggtagggaaa tggcacctga cgacactcct tgattacggg aggcgacatg gcaggactga
     4141 gctcaatcac aaacatcgtc gtgctgatgt tcgagaatcg ctcctttgac aacatcttcg
     4201 ggaacctcta cctgaacggc agcactgtga atggctacag cttcgagggc gtaacggccg
     4261 gcaccgagcc gaacgattcc aagccgtgga ccgacgttca gccggtcttg tggaacgacg
     4321 agatggtcac cactccgaat cccgatcccg gcgagatttt ccaggacgtc aacatgcagc
     4381 ttttcggcag cacgcaggac ggaccgccgt ggccgtcagc gtcgtcgctc ggaacgccgc
     4441 cgatgaacgc cttcgtcaac aactacacat tgcaaagcga tccaaacggg ccgctgtcat
     4501 cggaggtgcc gcgcaagcgc tcctggggac ctcccggata cgcgggcgcg ggaaccgatg
     4561 cggtgggctc cgacatcatg cactgcttca actacggcac gctcccggtg tcgacgttgc
     4621 tggccacgca gtatgcggtc agcgatcaat ggttcgcctc ggtgccgacg cagacctatc
     4681 cgaaccggat gtacgcacat tgcgcgacga cgtggtccag ggatctggtt caccattaca
     4741 aggagtacta cgacgactgg gacatggtcc tgctccactc cgtgacgaac tcgattttcc
     4801 aggagctcga cgcgcagggc gcggcgggga atctgcggaa tgcgacggcg ccgtactgga
     4861 aggtgtatta cccgagaaag gccggcagct tttcgatctc cgaggcgctg ctccactatg
     4921 tctcgggatc atcgcaggtg gtcgacatca gcgagctcgc cgccgacgcg ctggccggca
     4981 cgctaccgct ctactcgttc atcgagccgc cttacggaat gcccaacccc cacatcagca
     5041 aaatccccat cgactcgagc tatcacccgc cgtacgacgt cctgcaagga gagcagtttc
     5101 tccagcaggt ctacctgacg ctcttcgatc cgtcgaacaa tccgaactgg gaaacgacgc
     5161 ttctgatcgt caccttcgac gagcacggcg gcacgtacga tcacgttcct ccgccgccag
     5221 cgccgctgcc gaacgccgcg gcatataggc tcgatggcaa ggacgttccg gcaaacccga
     5281 gcgcgcctcc gttcgaccgc tacggcgtgc gcgtgccgtt cttcgtcgtg tcgcggttcg
     5341 cgcgacgaaa ctacgtgagc cacgtggtcg ccgaccacac ctcgatcacc cgcttcatcg
     5401 agaaccgctt cgacctgggc gcgatgacca agcgcgacgc caacgcctgg ccgctgctcg
     5461 atctgttcga cttcgagcac ccgccgttcg acgacccacc gacggaggag gtgctcgccg
     5521 agccctcgcc cgccggg
//
LOCUS       DATLVW010000885         8655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_371832, whole genome shotgun sequence.
ACCESSION   DATLVW010000885 DATLVW010000000
VERSION     DATLVW010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371832"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VNN25_26220"
     CDS             <1..1432
                     /locus_tag="VNN25_26220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH95097.1"
                     /translation="TLRGRLIAMVAALVAVMIGSVAVISTRVAHYEIRKFEVAVTTGG
                     TPRLPPTTSFQIDRLTPRTPGTIAVFYREHGTWAGVQPAVDQMAKTAGTDVLLFDRDR
                     RLVASSNNQLRIARIDLLASGMLAIERNISSGKTNELIRGPQIVIRDASGEIAGSLFL
                     LPHRNIALELPRTRSLDRSFFWIFLGAMLFGVVIAVAIARAISSPVERLTAAARRMES
                     GDLAVRVEPGGGAELEELAHGFNAMAATLARNEELRRRMVSDVAHELRAPLTNIRCEL
                     ESMQDGLTALTPERIASLHDETMHLTSLVDDLQDLALAEAGRLDIDPQPIAVAALARR
                     AAAGIEPRARERGVTIRCEGDDDAVVLADARRTVQILTNLLTNAVAHMETAGEIRIAW
                     SRGDGEAVVQVIDSGVGIPADELPHIFERFYRVDVSRSRTTGGAGLGLSIVRQLVAAH
                     GGRVWAESDLGKGSTFSFTLPIQENL"
     gene            1553..3421
                     /locus_tag="VNN25_26225"
     CDS             1553..3421
                     /locus_tag="VNN25_26225"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXH95098.1"
                     /translation="MKIKLLLTTLLIAASAFAQNTTDEQRVTQLNATVDKAVAEDRFS
                     GVVMLAKDGKPLLTRAVGMADPAKGIANRPDTKFNLGSINKFFTKIAIGQLAAVGKLS
                     LDDTIRKHLPDYPSPVADKITIAQLLEHRSGLGDIFGPKFMASHASLRKLSDYLPLFA
                     DAPLEFEPGTSQRYSNAGFVVLGLIIEKVSGQSYYDYVRDHITKPTGMTDTASYAVDE
                     NVPNRAIGQTKRGPEGPLPQRGNNTATLPGRGSSAGGGYSTAPDLLRLTQALLANKIL
                     SKGWTDWLFTDKFDSGGKRGLGVAGGSPGVNAVLEIAPPYALIVLSNFDPPSAEQVAR
                     EGRKIVGMGGEMKERRLGGPEPPGEIMIGKPVKIPMDITHHVPIIEAKINGKGPFRFD
                     VDSGFGGMMNISHALAEKLSLPVMGEAISGDPSGKNQKTVRVLHVESIDIADLHLGQV
                     DVTEAPADGPQREVDGVMGLPLFTSLLVTFDYPNSQFAVDGGKLPAADGSTVFAYSAE
                     HGVPTIEIDVAGTKVSTDIDSGSPALLSMPLSLAKSLPLAEEPRVVGHGRTVGNEFDI
                     YAAPLKGAVHVGRITVTDPMVDFVDIFPHANIGFRFLKDLVVTFDPAGNRVRFVKP"
     gene            complement(3506..4195)
                     /locus_tag="VNN25_26230"
     CDS             complement(3506..4195)
                     /locus_tag="VNN25_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HXH95099.1"
                     /translation="MKRLLLTIAVTAAFAMPMHAAGSSDALVASLITQAPGLRPNVLK
                     LALDAATRAEDRGLVPRRNLLTVIDYSLPSSQPRLFIFDLTAHKLLFRELVAHGKASG
                     GDVASFFSNEPGSLATSLGLFVTADTYNGGNGYSLRLRGLEAGVNDMAWDRMIVLHGA
                     SYVSQEAVKALGRLGRSWGCPAVRPEVSQKIIDTIRGGSPVFAYYPDKTWLSTSAFLS
                     QVASAITNASR"
     gene            4325..5587
                     /locus_tag="VNN25_26235"
     CDS             4325..5587
                     /locus_tag="VNN25_26235"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HXH95100.1"
                     /translation="MKRFLPPVILILCLSTLPIHADVFRLLESPHDGAQARVDVIQQA
                     HTEIGAVYFLARNDHITLTALGLLREARRRGVARVRLIVDANFQHIPKPVLAHLADEN
                     VEVRVYHPFTFCHLNWLFHRMHEKVVVVDGKRYITGGRNLAGSYFGLKKRNYIDRDVY
                     VEGPSAAAAQQHFEDLWNSSDVRELNVNVSPEAKAEAARILDDAALGKGLENLVTLNT
                     GRDWSQGLKDIPGVRFLSDPIAPAKGPRLAVRLAEVIEGAKRSVVIESPYLVPSPEVL
                     DLLRRKRAEGVRVQIITNSLRSCDGALPYVAYLKYRHRLLRAGVDMREYRGPDTLHAK
                     SLVIDGHIVLVGSYNIDQRSENQDREVMAEADDDDVARELMGAIRHHIDNSWAVGRGT
                     EGPHAPRSWRLRAWASKFLLIPFVEKQL"
     gene            5612..5965
                     /locus_tag="VNN25_26240"
     CDS             5612..5965
                     /locus_tag="VNN25_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95101.1"
                     /translation="MYETRRQKLLTRAEFALRVLWHFTFGVGVAAIALFAGMLGYRYL
                     AGLSWVDSLLNASMILGGMGPVDTLKTNASKIFASFYALFSGLVFIGLLGVLLAPFIH
                     RVMHKLHIDERGTKG"
     gene            6133..6672
                     /locus_tag="VNN25_26245"
     CDS             6133..6672
                     /locus_tag="VNN25_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HXH95102.1"
                     /translation="MALTATMYRFGIQLSDVDRGVYETLELPIARQPSETAEYMLTRV
                     LAWCLEYTEGIALTEGVASGDEPAVLVRDLTGRITAWIEVGAPDADRVHRGSKLAGRV
                     AIYTHRDPAMVLGQLAGKKIHRGDEIPIYTFGRGFIESVAAILPRRASLSITVTERQL
                     YLDVDDQSFSTTLEERRAG"
     gene            6920..8413
                     /locus_tag="VNN25_26250"
     CDS             6920..8413
                     /locus_tag="VNN25_26250"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH95103.1"
                     /translation="MGRSLFGKLHRRFGGRVSGQERQRRAAEHYERLRQAMPVGLVGA
                     PRRIATKNPRVAVLGAGFAGVSAGWFASRAGFAVTLIEPLVVGGRVSSTDKLVPGRIL
                     EAGAELIGTNHPLWLALAAHFGLAMSVVSSEDNYAAAGLDMPMILDGRSLSTAEQEKL
                     HAEMDTVFRQWGAESTVVRNPWMPWTTLRAAELDAVSLASQIPAGVSELARKAILLEF
                     ELDNCTPAEQQSWLGALSQLAAGGGYAFFDETEVFRCAAGNQSLARRLGAGIDLNQGR
                     ATNIRTSGDRLFVELAGGAVDGPYDFAVVAVPSVAMEKITIDGKPFPYRAIAHGPAVK
                     YLSGVERRFWIPEGVAPSGMSDQLGMIWEGTDNQMDTARFDVSVFAGGRPAQHAIDAG
                     GGPSYFAPRLNALLPHYEAFTTEFENWPKRIGMGYSTPAVGEVTTKQKAYADLIAGRI
                     AVAGEHTSPAWFGFMEGALESGLVAMARIANIAGIPLAPEYGDFHAV"
BASE COUNT         1632 a         2795 c         2654 g         1574 t
ORIGIN      
        1 cacactgcgc ggccgcctca tcgccatggt cgcggcgctc gtcgcggtca tgatcggcag
       61 cgtggcggtg atctctacgc gcgtcgcgca ctacgagatc cgtaagttcg aggtcgccgt
      121 gaccactgga gggactcctc gcctgccgcc tactacgtca tttcagattg accggctgac
      181 gccgcggacg ccaggaacga ttgccgtgtt ctaccgcgag cacggcacgt gggccggcgt
      241 acaaccggcc gtcgatcaga tggcgaagac cgcgggaacc gacgttcttc tcttcgatcg
      301 tgaccggcgt ctggtggcgt cgtcaaacaa ccaacttcgc atcgcgcgga tcgatctcct
      361 ggccagcggg atgctggcga tcgagcgcaa catttcgtcg ggcaagacga acgagttgat
      421 ccgcgggcca caaatcgtca tccgcgacgc aagcggagaa atcgccggtt cgctcttcct
      481 gctgccccac cgaaacatcg cgctggaact gccgcggacc cgctcgctcg atcgctcttt
      541 cttctggatc tttctagggg cgatgctgtt cggagttgtc atagccgttg ccatcgctcg
      601 cgccatcagc agcccggtcg agcggcttac cgcagcggcg cggcggatgg agtcgggcga
      661 tctggccgtg cgcgtcgagc ccggcggcgg cgcggagctc gaagagctgg cgcacggctt
      721 caacgccatg gccgccacgc tcgcccgcaa cgaagagctg cggcggcgca tggtgagcga
      781 cgttgcgcac gagttgcgcg cgccgctgac caacatccgc tgcgaactgg agtcgatgca
      841 ggatggcctc accgcgctaa ctcccgagcg cattgcctca ctccacgacg agacgatgca
      901 cctcaccagc ctcgtcgacg accttcagga tctcgcgctc gccgaagcag gccgcctcga
      961 catcgatccg cagccgatcg ccgtcgctgc actggcgcgc cgcgctgcgg ccgggattga
     1021 gccgcgagcg cgcgagcgcg gcgtgacgat tcgctgcgag ggcgatgacg acgcggtagt
     1081 cctcgccgac gcgcgccgca ccgtacagat cctcaccaat ctcctgacca acgccgtggc
     1141 gcacatggaa accgcgggcg agattcgcat cgcatggtcg cgcggcgatg gcgaagcggt
     1201 cgtgcaagtg attgacagcg gcgtcggcat tccggcagac gagctcccgc acatcttcga
     1261 gcgtttctac cgcgtcgacg tctcccgttc ccgcaccacc ggcggcgccg gcctcggcct
     1321 ctccatcgtc cgccagctcg tcgccgcaca tggcgggcgc gtttgggcgg agagcgacct
     1381 cggaaaggga tcaacctttt cgttcacgct gcctatccaa gagaaccttt aaccacagag
     1441 acacagaggt cacagagaaa acctctgtga cctctgtgtc tctgtggtta agggttttca
     1501 taacttcatc agcacttcac aactgaaccg taccttgctc ccggaggcac tgatgaaaat
     1561 caaactgcta ctcaccacct tgctgatcgc tgcgtcggcg ttcgcacaga acacgacgga
     1621 cgaacagcgc gtcacccagc tcaacgctac cgtcgacaaa gcggtcgccg aggatcggtt
     1681 ctcgggcgtc gtgatgctgg ccaaagacgg caagccgctg ctcacgcgcg ccgtcggcat
     1741 ggccgatccc gccaaaggaa tcgccaaccg cccggacacg aagttcaacc tcggctcaat
     1801 caacaagttc ttcaccaaga ttgcgatcgg acagctcgcc gccgtcggga agctgtcgct
     1861 cgatgacacc atccgcaagc atctgcctga ctatccatcg ccggttgcag acaagatcac
     1921 catcgcccag ctcctcgagc atcgctccgg cctcggcgac atcttcggac cgaagttcat
     1981 ggcctcgcac gcatcgctgc gtaagctctc cgactatctc ccgctcttcg ccgacgcgcc
     2041 gctcgagttc gagcccggca catcgcagcg ctactcgaac gccggcttcg tcgtcctcgg
     2101 cctcatcatc gagaaagtgt cgggacagag ctactacgac tacgttcgcg accacatcac
     2161 gaaacccacc ggcatgaccg acaccgcgtc gtacgcggtc gatgaaaacg ttccgaaccg
     2221 cgccatcggc cagaccaaac gcggtcccga gggtccgctg ccgcagcgcg ggaacaacac
     2281 cgcaacgctg ccggggcgcg gcagctcggc gggcggcggc tactcgacag cgcccgatct
     2341 gctccgcctc acgcaggccc ttctcgccaa caagatcctg tcgaaaggtt ggaccgactg
     2401 gttgttcacc gacaagttcg attccggtgg taagcgcgga ctcggcgtcg ccggaggttc
     2461 gcccggcgtc aacgcggttc tcgagatcgc gccgccctac gcgctcatcg tcctctccaa
     2521 cttcgatccg ccgtccgccg aacaggtggc gcgtgaggga cgcaaaatcg tggggatggg
     2581 cggcgagatg aaggagcgcc gtctcggagg tccggaacct ccgggcgaaa tcatgatcgg
     2641 caagcccgtg aagatcccga tggatatcac gcatcacgtg cccatcatcg aagcgaagat
     2701 caacggcaaa ggaccgttcc gattcgacgt cgacagcggc ttcggcggca tgatgaatat
     2761 cagccacgca ctcgcggaga agttgtcgct gcccgtgatg ggcgaagcga tcagcggcga
     2821 cccgagcggc aagaatcaga agacggtccg cgtcctccac gtcgaatcga tcgacattgc
     2881 cgatctgcac ctcgggcaag tcgacgtcac cgaggcgcct gcagatggcc cgcaacgcga
     2941 ggtggacggc gtgatgggat taccgctctt cacatcgctg ctggtgacgt ttgattaccc
     3001 gaacagccaa ttcgcagtcg atggcggaaa acttcccgca gcggacggca gcacagtttt
     3061 cgcctactcg gccgagcacg gcgttccgac gatcgagatc gatgtcgccg gcacgaaagt
     3121 gtcgaccgac atcgacagcg gcagcccggc actcctgtcg atgccgctct cactcgccaa
     3181 atcgttgccg ctcgcagaag aaccgcgcgt cgtgggacac ggacgaaccg tcggcaacga
     3241 attcgatatc tatgcggcgc cgctgaaggg cgcggtccac gtcggccgga ttacggtcac
     3301 cgacccgatg gtcgatttcg tcgacatctt cccgcacgcg aacatcgggt tccgattcct
     3361 gaaagacctc gttgtgacgt tcgacccggc gggcaatcgg gttcggttcg tgaagccgta
     3421 agacgagagt gaccaccaca gagacactga ggacacagag aagtactctg taaactcggt
     3481 gtctctgtgg ttgaagggtt tgcggttacc gcgacgcgtt cgtgatcgcg ctggcgacct
     3541 gcgagaggaa cgccgaggtt gagagccagg tcttgtcggg atagtacgca aacaccggac
     3601 tgccgccgcg aatcgtatcg atgatcttct gcgacacttc cggccgcacc gcggggcaac
     3661 cccagcttcg tccgagacgg ccaagcgctt tcaccgcttc ctggctgacg tacgacgctc
     3721 cgtgcagcac gatcatccgg tcccaagcca tgtcgttgac gcccgcttcg agaccgcgca
     3781 ggcggagcga atagccgttg ccgccgttgt aggtgtcggc ggtgacgaaa aggccgaggc
     3841 tggtggcgag gcttccgggc tcgttggaga agaacgaggc gacgtctccg ccgctcgctt
     3901 ttccgtgggc aaccaattcg cggaagagga gcttgtgcgc ggtcaggtcg aagatgaaga
     3961 gacgcggctg tgacgacggc agcgagtagt cgatcaccgt cagcaggttt cttcgcggaa
     4021 cgagtccccg atcctcggcg cgcgtggctg cgtcgagcgc cagcttcaga acgttgggac
     4081 gaaggccggg cgcctgcgtg atgagtgagg cgacaagggc gtcggatgag cccgccgcgt
     4141 gcatcggcat agcaaacgcg gcagtcacag caatggtgag gagcagtcgc ttcattggtt
     4201 ttccggacga tcggatttgg acggggatga taacaaataa cccgggagag tattgcgcga
     4261 tcggacaaca ccggcgcggg gacgccggca atccttggtt catctcatgc agtaggcgga
     4321 gcgcatgaag cgtttcctac ccccggtcat cctgattctt tgcctctcaa ctcttcccat
     4381 tcacgccgat gtctttcgtc tgctcgaaag tcctcacgac ggcgctcagg cgcgcgtcga
     4441 tgtcattcag caggcgcaca ccgagatcgg tgccgtctac tttctggccc gcaacgacca
     4501 catcacgctc actgcgctcg gcctgcttcg cgaagcgcgg cggcgtggcg tcgcgcgcgt
     4561 acggctgatc gtcgacgcca actttcagca cattcccaag cccgttctcg cgcatctggc
     4621 cgatgaaaac gtcgaggtgc gcgtctatca cccgttcacc ttctgccatc tgaactggct
     4681 gtttcatcgg atgcacgaga aggtggtcgt cgtcgacgga aagcgctaca tcacgggtgg
     4741 ccgtaatctc gccggttcgt acttcgggct gaagaaacgc aactacatcg atcgcgatgt
     4801 ctacgtcgaa gggccctcgg cagccgctgc ccagcagcac ttcgaagatc tctggaacag
     4861 cagcgatgtc cgcgaactga acgtgaacgt ctcgcccgag gcaaaggcgg aagcggcgcg
     4921 cattctcgac gacgccgcgc tgggcaaagg gctggagaat ctcgtcacgc tgaacacggg
     4981 acgcgactgg tctcagggac tgaaggacat ccccggcgtt cgttttcttt ctgatccgat
     5041 cgcaccggca aaaggcccac gcctggcggt tcggctcgcc gaggtgatcg aaggcgcgaa
     5101 gagatcggtg gtgatcgagt cgccgtatct cgttccatcg cccgaagtcc tcgatctcct
     5161 gcgcagaaaa cgcgcggagg gcgtgcgcgt ccagatcatt accaactccc tgcgctcctg
     5221 cgacggtgct ctgccctatg tcgcctacct caagtatcgg cacagactgc tccgtgcagg
     5281 ggtcgatatg cgcgagtacc gcgggcccga cacgcttcat gccaaatcgc tcgtgatcga
     5341 cggccacatc gttctggtcg ggtcgtacaa catcgatcaa cgttcggaga atcaggaccg
     5401 agaggtgatg gccgaggcgg atgatgacga cgtcgcgcga gagttgatgg gcgcgatcag
     5461 acatcacatc gataactcat gggccgtcgg gcgcggcaca gagggccccc acgccccgcg
     5521 ctcgtggcgg ttgcgcgcgt gggcgtcgaa gttcctgctg attccttttg tcgagaagca
     5581 gttgtagcgc gtggtctaat cgcgaggatc catgtacgag actcggcgac aaaaactcct
     5641 gacccgcgcg gaattcgcgc tgcgagtcct gtggcacttc acctttggag taggcgttgc
     5701 cgcgatcgcc ctcttcgccg gaatgcttgg atatcgttat ctcgccggcc tatcgtgggt
     5761 cgattccctt ctgaacgcgt cgatgatcct cggcgggatg ggaccggtcg atacgctgaa
     5821 gacgaacgct tccaagatct tcgcgtcgtt ctacgcgctc ttctccggac tcgtcttcat
     5881 cggcctgctc ggcgtcctcc tggcgccatt catccaccgc gtcatgcaca agttgcacat
     5941 cgatgagaga gggacgaagg ggtgaagctg ttctgagatc agagacaaga gatcagagat
     6001 acgggagatc agagagcgct cgcgttcttg gatctccaat ctcttgaatc tccggtctct
     6061 ttctccgatt ctctgatctc ttgtctctga tctcttgtct ctgatctctt gtctctgatc
     6121 tcactcaaaa ccatggccct caccgcaacg atgtaccgct tcggcatcca gctttcggat
     6181 gtcgatcgcg gcgtttacga gacgctcgaa ctacccatcg cgcggcaacc gtccgaaacc
     6241 gcggagtaca tgctcacgcg cgtgctggcg tggtgcctcg agtacactga aggcatcgcg
     6301 ctgaccgaag gcgtggcctc aggcgatgaa cctgcggtcc tcgtgcgcga tctcaccggc
     6361 cgcatcaccg catggatcga agtcggagct cccgacgccg atcgcgtaca ccgcggaagc
     6421 aagctcgccg gccgcgtcgc gatttacacc catcgcgatc ccgccatggt cctcggccag
     6481 ctcgccggaa agaagatcca ccgcggcgac gagatcccga tctacacctt cggccgcggc
     6541 ttcatcgaaa gcgtggccgc catcctcccc cgccgcgcgt cgctatcgat cacggtcacc
     6601 gagcggcagc tctatctcga cgtcgacgac cagtcgttct cgacgacgct cgaggagcgg
     6661 cgcgcggggt gatgagcgag acctccgccc ctactgcact ggggcgagga cgcccgagtg
     6721 cccgcggggc gtcccgcctc gatggttgga tccggactga tagcccagtg gtttgaaacg
     6781 ggtcaagatc gaaaaaatag atctggcgat aatcttcgcg gtcgctacca gccctgatgg
     6841 tgaacggtca cgcgcccgaa cgacccccgc ctgattccac tccaaacccg ctatccttgg
     6901 gccgcacgga gggcatcgta tgggccgctc actcttcgga aagttgcatc gtcggttcgg
     6961 gggccgcgtt tcgggacagg agcggcagcg gcgcgcggcg gaacattacg agcggctgcg
     7021 acaggcgatg ccggtcggac tcgtcggcgc gccgcggcgg attgcgacga agaatccgcg
     7081 ggtggctgtg ctcggtgccg gattcgccgg cgtgagcgct gggtggttcg cgtcgcgggc
     7141 aggattcgcc gtcacgctca tcgaaccgct cgttgtgggc gggcgtgtat cgtcaacgga
     7201 caagctcgtg ccgggacgca tcctcgaagc gggcgcggag ttgatcggga ccaaccatcc
     7261 gctctggctc gcgcttgccg ctcattttgg tcttgccatg agcgtcgtct cctcggagga
     7321 caactacgcc gccgcggggc tggacatgcc gatgatcctc gatggccgca gcctttccac
     7381 cgccgaacag gagaagctcc acgcggagat ggatacggtc ttccgccaat ggggggccga
     7441 gtcgaccgtg gtcaggaatc cgtggatgcc gtggacgacg ctgcgagcgg cagagctgga
     7501 cgccgtgtcg ctagcctcgc agattcctgc cggtgtcagc gagctggcga ggaaggcgat
     7561 cctgctcgag ttcgagctcg acaactgcac tccggcggag cagcaaagct ggctcggtgc
     7621 actgtcacag cttgcggccg gcggcggcta tgcgttcttc gatgagaccg aagtctttcg
     7681 ctgcgccgcc ggcaaccaat ccctcgcccg ccgcctcggc gccggtatcg atctcaacca
     7741 gggacgggcg acgaacatcc gcaccagcgg cgaccgcctc ttcgtcgagc ttgccggagg
     7801 cgcagtcgac ggaccgtacg acttcgcagt cgtggccgta ccgtccgtgg cgatggagaa
     7861 gatcacaatc gacggcaagc ccttcccgta tcgcgccatc gcccacggcc cggccgtgaa
     7921 gtacctctcc ggcgtcgaac gccgcttctg gattccggaa ggagtcgcac cgagcggcat
     7981 gtccgatcaa ctcgggatga tctgggaagg aaccgacaat cagatggata ccgcgcgttt
     8041 cgacgtgagc gtcttcgccg gcggccggcc tgcgcagcac gccatcgacg cagggggcgg
     8101 tccgtcctac tttgcccctc gtctcaacgc gctccttccg cactacgaag cattcacaac
     8161 cgagtttgag aactggccca aacgcatcgg catggggtac tccacacccg cggtcggcga
     8221 agtgaccacg aagcagaagg cgtacgccga cctgatcgca ggccgcattg ccgtcgcggg
     8281 cgagcacacc tcaccagcat ggttcggctt catggaaggc gcgctggagt caggactcgt
     8341 agcgatggcc cgcatcgcaa acatcgccgg tatcccgctc gcgccggagt acggagactt
     8401 tcacgcggtg tagaagtcct tcggtcacca cagagactca gagtacgcag agacctctgt
     8461 gctctcagta tctcagtggt gaatggtttt atgttttctt cagctcaccg ccagaccgag
     8521 agaatcatgc ccgccgcaat gaacgagaca aggttgtagg cggagttgat gaaccagagc
     8581 ggcttcttcg tcatcgagaa aatggcggtg gcgaagctcg tcggcgccgc gaatccgacc
     8641 cagcagatcg ttccc
//
LOCUS       DATLVW010000886         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_82182, whole genome shotgun sequence.
ACCESSION   DATLVW010000886 DATLVW010000000
VERSION     DATLVW010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82182"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..1143
                     /locus_tag="VNN25_26255"
     CDS             454..1143
                     /locus_tag="VNN25_26255"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH95104.1"
                     /translation="MKKPPTKKPKRRHRFLPLAIGTTGSVALAGVAFWFFQKQAEQVV
                     QGRADRYDEAIMDAVHTIDNPQMHQVMHAITQLGTHVAIGSAAGITALAMWRKGRRDD
                     AWTVVVSTGGAMAINTILKNIFQRQRPIERARRIKLPGSHSFPSGHSLLSAATYPIVA
                     HHFVQHESVATQAVVHTLAGLTILSVGFSRVYFGVHFPSDVFGGFAAGIGWLGLTSLS
                     HSLMEKKKDEG"
     gene            1561..>2019
                     /locus_tag="VNN25_26260"
     CDS             1561..>2019
                     /locus_tag="VNN25_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95105.1"
                     /translation="MPRIEIDTVLKLPSNATVAGTSFVGEPSLGMHGQQILFTGNWYV
                     ARSADRGATWEGFDPNDYFPQNPPTPFCCDQSVLYVPQHDLFVWLLQYEKSASGNTLR
                     IAVKRVSKPLHEGEWFTWDLSPTDVNEEWTKEWFDYNHLAATDRHLFIASN"
BASE COUNT          403 a          677 c          570 g          369 t
ORIGIN      
        1 gtttgaccgc cgtcccgtcg cgtttgaccg ccgtcccgtc gcgtttgacc gccgtcccgt
       61 cgcgtttgac cgtcgtcccg tcgcgtttga tcgccgttcc gtcgcgtttg accgagttcc
      121 gtcgcgtttg accgcggttc cccgcgcttg aacgcggtgt tctcgtgttt atctgtgaaa
      181 aatcgcgcct gtccacagga ctgtcacgat catcggtccg gttttgcaag ctcgggtatg
      241 acaatcgcga agcggcgtgt cgccgtcgct gccagccgca aagccccctg ccgcgcgcac
      301 aacctccgct tcgatccaat gactcgcccc aaccccggta ctccgacacc cgacccccga
      361 ctcccgactc ccggcctaac ccaggctagt agccaacagt aactccaggc gcccctcccg
      421 gcctcacagc cacccctgtt atagactcgc cccgtgaaga aaccgcccac gaagaaacca
      481 aaacgccgcc atcgattcct accgctggcg atcgggacga ccggcagcgt cgcgctggca
      541 ggcgtggcgt tctggttctt tcaaaagcag gccgagcagg tcgtgcaggg gcgcgccgac
      601 cgttacgacg aagcgatcat ggacgcggtc cacacgatcg acaatccgca gatgcaccag
      661 gtcatgcacg ccatcacaca actcggcacg cacgtggcca taggcagcgc ggcagggatc
      721 acggcgctgg cgatgtggcg gaagggccgg cgcgatgacg cctggaccgt cgtcgtctcg
      781 accggcggag caatggccat caacacgatc ctgaagaaca tctttcaacg ccagcgtccg
      841 atcgagcgcg cccgccggat caagttgccc ggctcgcaca gcttccccag cgggcactcg
      901 cttctgtcgg cagccacgta tccgatcgtc gcccaccact tcgtgcagca tgaatcagtg
      961 gctacgcagg ccgtcgtgca cacgctggcc ggcctgacga tcctcagcgt tggtttttcg
     1021 cgcgtctact tcggcgtcca cttcccgtcc gacgtcttcg gcggattcgc cgccggaatc
     1081 ggctggctgg ggctaacgtc gttgtcgcat tcgttgatgg agaagaagaa ggacgagggt
     1141 taatcgcctc tctgtccttc gcgattcgct ccgtcaatga gtctataatt ctgacccagg
     1201 gaagacgcgg ccccgaggat tgttttccca gaacggaagc ataaatggta aacctccaga
     1261 cagaaatgcc agcgcagatg gacaaaacga gcaccgagcg cggcgaaact gtcagatcga
     1321 acaggcgtgt gatcggcgca ccgcgagtac aagccgccgc cagcgcgagt gctgtccgtg
     1381 aggatcgccg gccgccatcg cgagaacaaa ccgaagcccg tcagcgagcg atgtccgaaa
     1441 cgaggcgccc cacgaaaccg ctcgaagccg cgctcgcgga aaagaacccg aagaccatct
     1501 cggttcctga cgtggccgcc acggattgtg gcaagagcaa gtagtcgcgg gtatcgcgct
     1561 atgccgcgga ttgagatcga caccgttctc aagctcccct caaatgccac ggtcgcaggc
     1621 acatcgtttg tcggcgaacc aagtctgggc atgcacggcc agcagattct attcaccggc
     1681 aactggtacg ttgcccggtc ggctgacagg ggcgcaacct gggaaggctt cgatcctaac
     1741 gattactttc ctcagaaccc accgactccg ttttgctgcg atcagagcgt gctctacgta
     1801 ccgcagcacg atctcttcgt ctggctcctt cagtatgaga agtccgccag cggcaacaca
     1861 ctccgcattg cagtaaagcg cgtttccaag ccgctgcacg agggcgagtg gttcacgtgg
     1921 gatttgtcgc caaccgacgt caacgaggaa tggacgaagg agtggttcga ctacaaccat
     1981 ctcgctgcca ccgaccgtca cctcttcatc gcctcaaac
//
LOCUS       DATLVW010000887        10273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_448542, whole genome shotgun sequence.
ACCESSION   DATLVW010000887 DATLVW010000000
VERSION     DATLVW010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448542"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..617
                     /locus_tag="VNN25_26265"
     CDS             183..617
                     /locus_tag="VNN25_26265"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXH95106.1"
                     /translation="MNARAEHFVSNINSDDLTIGELARRAGMSTSAIRYYESVGLVEP
                     KRRSSGRRLYGEFAVERLALISFAKDIGFSLGQVRQLLAGFPEETPASARWTELATSK
                     LAELDVEAHRIEVMRAALLRIMRCRCDDVDQCAHGIAASRCR"
     gene            645..1997
                     /locus_tag="VNN25_26270"
     CDS             645..1997
                     /locus_tag="VNN25_26270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HXH95107.1"
                     /translation="MKRSIAVIALLLLSQTMFAATPPIDRVAMQAKVRAEFLYSWKAY
                     EKYAWGHDELRPLSKQPKDWYGESLLMTPVDSLDTLLLMGLNDEADKAKTLIVEHLSF
                     DKDISVKVFEITIRELGGLLSAYEMTGDARLLHLADDLGTRLLPAFNTPTGMPYMYVN
                     LRTGKTSGARSNPAEVGTLILEFGTLSKLTNKPVYMEKAKGALVELYKRRSKIGLVGE
                     EIDVETGAWVSKASHVGGGIDSYYEYVLKCSRLFGDQECESMWQTSIRALNKHLAEEA
                     PSGLWYGEVDMNTGKRTNPEFGALHAFLPAVLELGGDTKRARRLEESCFRMWTLSGVE
                     PEVLDYRTMTIKSPGYQLRPEIIESAYYLHHGTHDARYLMMGQTFFDAIVAHCRTDAG
                     YTTLKSVVTMEKGDLMPSYFLAETLKYLYLLFAPDSALDLDKVVFNTEAHPLKRELKP
                     "
     gene            2282..2905
                     /locus_tag="VNN25_26275"
     CDS             2282..2905
                     /locus_tag="VNN25_26275"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXH95108.1"
                     /translation="MVYIDGYNLYHGLRDRHGRKYLWLDLTRFARGLLSDPRQELVSV
                     KYFTSAAPGSRDGAKRQQIYWKALETCAGLQIIKGRYREKPTRCSVCGTKEAECRICG
                     AKLRFRNEKMTDVNIATHLVRDACSGTFDVAVLVGGDTDLLAAVEAAKALRRIVVAFP
                     PDRENDDMKRAASGWFRLSEGKFKTAQFPDVVTVAPGITVTRPVLWR"
     gene            2963..3439
                     /locus_tag="VNN25_26280"
     CDS             2963..3439
                     /locus_tag="VNN25_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95109.1"
                     /translation="MNLPSILTWGLAATIVLTTLSIAAQSLGFTRIDIPFIVGTMFTP
                     NRDRAKVLGLGMHIINGWIFALVYAFAFESRHMASWWFGGMIGAVQGMFIVIVLLPVL
                     PGLHPRMVSDFRGPEPTRLLEPPGIFTTNYGIKTPFVLLFAHMVYGVVLGTFYTLR"
     gene            complement(3441..5321)
                     /locus_tag="VNN25_26285"
     CDS             complement(3441..5321)
                     /locus_tag="VNN25_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXH95110.1"
                     /translation="MAETLGKVIDPYVPIADYAAIGCTRSIALLSRRGSIDWLCWPRF
                     DSESVFGRILDTEKGGYFAIQPAIPFESKRRYLEGTNVIETTFTTASGVVRLLDLMPV
                     MTELEKQKRLSPFRQLLRRAECVDGEVPITVTYAPRPDYGRITPSLKVRNDSVSCAWR
                     ARVLNLRSDAEFLVERGTATSSFNLRAGESRTFALGFDDHTPVVLAPIGDEATAEIEK
                     TIGFWREWSGQLTYDGPYRDLVLRSALALKLLTYTPSGAIVAAATTSLPESIGAERNW
                     DYRYCWLRDASWTVSALYDCGFSNEGEAFLDWLLYSTRLTQPNLQMLYDVFGEAHIPE
                     KTLDYLDGYCGSRPVRIGNAAHAQFQLDVYGEVIGAVDEATQRGATLDRDSQRLLRRL
                     TEIVCERWREPDAGIWEKRGGARQHVHGKVMAWSALDSALKIADRCHIKVGRPHLEQT
                     KAAIKEAVLTRGFDAERNTFVGIFDEKEVDASLLFIARVGLIDPKDPRMLGTIDAIRS
                     TLGGDGLLYRYDTRKTEDGLPAGEGAFVACTFWLVEALALAGRVDEAHALFDDVVRHA
                     NDAGLFSEEVDVHTGALLGNFPQALTHIALLNAALALQEASRKGEAKPRASASPRETV
                     GT"
     gene            complement(5306..8527)
                     /locus_tag="VNN25_26290"
     CDS             complement(5306..8527)
                     /locus_tag="VNN25_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXH95111.1"
                     /translation="MSPVSDTPHVHAEDLAVSAAASPKDRDERTRFDPVAFEHDRPVR
                     TRIEHSDRVQTLNVDAALLAADLRREVRGEVRFDDGARALYATDASNYRQVPIGVVVP
                     RDAHDVVATVALARKHGAPILARGGGTSLAGQCCNVAVVLDFSKYMHAIPEMNPEARF
                     ARVQPGIILDDLRNAAEKHKLTFGPDPATHAYCTLGGMIGNNSCGVHALMAGKTVDNI
                     EELDILTYDGVRMRVGATSDSELEQIIAEGGRRGEIYAGLKRIRDTYGPLIRAKYPKI
                     PRRVSGYNLDALLSENGFNVAQALVGSECTCVVVLEAKCRLVDSPPSRTLVVLGYDDI
                     YLAADDVPRLLEFRPIGLEGIDDRLVEDMRAKGLNIDKLHLLPEGKGWLLVEFGGATK
                     EASDAYARTMIASLAASPLKIAHRVCNDRDAKLVWAIRESGLGATAFVPGKGHTWEGW
                     EDSAVDPKHLGAYLRELRVLLNKYGYSGDLYGHFGQACVHTRNNFDLESAAGIAKFRA
                     YLDEAADLCIKYNGSLSGEHGDGQARADLLPKMFGPELIQAFNEFKSLWDPEWKMNPG
                     KVVKPYHPTENLRLGADFKQWSPATHFQFPDDHGRIDSAVGRCVGVGKCRRLDGGTMC
                     PSFMVTREEEHSTRGRARLLFEMFEGQVIPADWNNEAVKDSLDLCLACKGCKGECPVN
                     VDMATYKAEFLSHYYERKLRPVAAYSMGWIYWWAQIASLAPGLANAIMSLPVFRKLGG
                     IAPERTMPHFAKRTFVEQFKRRSDSQSDKTKRRTRESDLRSSDLRSSDLRKVLLWPDT
                     FNNHFHPGTANAATEVLEAAGFEVIIPRKRLCCGRPLYDWGFLGMAKKLLLETMEALR
                     PELDAGIPIVGLEPSCISVFRDELPNLFPGNDVATRLSKAVKTLPEFLLQEGVELPKL
                     QGKALVQAHCHHKAVLHFDDEAQVLGKMGLEVDQPDSGCCGMAGAFGFEPDKYELSMR
                     VGERVLLPAVREAARNTIIVADGFSCREQIAQGTGREALHLAQVLHLAMKQNAPGALP
                     ERGYADEPAPADIKKVAWGAAGVAAGIALAAGVLALLRRWRKR"
     gene            complement(8639..9412)
                     /locus_tag="VNN25_26295"
     CDS             complement(8639..9412)
                     /locus_tag="VNN25_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95112.1"
                     /translation="MNYFLSAALAFSLLSSGVSAQSISRRTVPIDAATAPANPRLLVP
                     VLVSNLPGAMGSVWQTDMWITNVSSQPIVYSTNPCNQACCCAEINTIRPQSTIRYAEN
                     RPQGRYFATVDSIQLEVRLRDLTRNASSAGVNLPVVREDEFRADEMNLIAVPRDPRFR
                     VLVRFYMLDKALPLSVEQIDASGNVLQADQVQLNPPVGGFAVPAPAYGQFSLDPGTQE
                     AAEPVRIRVKAIYPNTYFWAFASVTNNDTSEVTLILPPR"
     gene            complement(9547..10086)
                     /locus_tag="VNN25_26300"
     CDS             complement(9547..10086)
                     /locus_tag="VNN25_26300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95113.1"
                     /translation="MQASIKRVFAYAIIVVSVVALRTGVLSACQRFGPVVPEDMVASA
                     DLIVRATVDYSRMPTAFGRTSWRPESTVRFRIEEVVKGRYALTDLDLPGYLESSDDFN
                     ELPVPYTFVRRGGRGGSCFANTYRQGGRYLLVLKMVAGAYTVDWYALGPTNEQLHSVD
                     DPWLKWVRSQVGPRIRSLR"
BASE COUNT         2034 a         3129 c         3196 g         1914 t
ORIGIN      
        1 tgcaaggatc gactgacgaa aggcggctag cgaaaagcga aatgacggcg acaaggaagt
       61 agaacggatt catgggtgat tcctccagcc atgcaaggta ggacttcgag tcagcttgaa
      121 gtcaagggct cctgctgtga actgttcgtg acgcatccga ttcgcagccg gatcgtgtga
      181 tcatgaatgc gcgagcggag cacttcgtga gcaacatcaa cagcgacgac ttgacgatcg
      241 gtgagctggc tcgccgggcc ggcatgagca cttctgccat tcggtactac gagtcggtgg
      301 gtctggtcga gccaaagcgc aggagcagtg gccggcgttt gtatggagag tttgctgtcg
      361 agcgtctggc gctcatctcg ttcgccaaag acatcggatt cagtctcggg caagttcgac
      421 aactcctggc cggattcccg gaagagacgc cggcgagcgc gcgatggacc gagctggcaa
      481 cttccaaact ggcggagctg gatgtggaag cacaccgcat cgaagtcatg cgcgccgcgt
      541 tgctgcgaat catgcgttgc cgctgcgacg acgtcgatca gtgcgcgcac gggatcgcgg
      601 cgtcccgttg ccgatgatcg cggcgcgctc gctatactcg ctctatgaag cgaagcatcg
      661 ccgtcatcgc gctcctcctg ttgtcgcaaa ccatgttcgc ggccacgcca ccgatcgatc
      721 gggtggccat gcaggccaaa gtccgcgccg agtttctcta ttcgtggaag gcgtacgaga
      781 agtacgcctg gggccacgat gagctgcggc cgctgagcaa acaaccgaaa gactggtacg
      841 gcgaatcgct cctgatgacg cctgtcgatt cgctcgacac gctgctcctc atgggactca
      901 acgatgaagc ggacaaggcg aagacgctga tcgtcgagca tctctcgttc gataaagaca
      961 tctcggtcaa ggtcttcgag atcacaatcc gcgagctcgg cggcctcctc tccgcttacg
     1021 agatgaccgg cgacgccaga cttctccacc tcgccgacga cctcggcacg cgcttactcc
     1081 cggcatttaa cacgccgacc ggcatgcctt acatgtacgt caacctgcgc acggggaaaa
     1141 cgagcggcgc gcggtcgaat cccgccgagg tcggaacgct gatcctcgaa ttcggaacgc
     1201 tcagcaaact gacgaacaag ccggtctaca tggagaaagc aaagggcgca ctcgtcgagc
     1261 tctacaaacg ccgctcgaag atcggcctcg tcggcgaaga gatcgacgtc gagaccggcg
     1321 cgtgggtcag caaggccagt cacgtaggcg gcgggatcga ttcgtactac gagtatgtgc
     1381 tgaagtgctc gcgccttttc ggcgaccagg agtgcgaatc gatgtggcag acgagcattc
     1441 gagcgctcaa caagcatctc gccgaggaag cgccatcagg tctctggtac ggcgaggtgg
     1501 atatgaacac cggcaagcgc acgaatcccg aattcggcgc gctccacgcc ttcctccccg
     1561 ccgtcctcga gctcggcgga gacacgaaac gcgcccgccg cctggaagaa tcatgcttcc
     1621 gcatgtggac gctcagcggt gtcgagcccg aggttctcga ctaccgcacg atgacgatca
     1681 aatcgcccgg ctaccagctc cgccccgaga tcatcgagtc cgcctactac ctccaccacg
     1741 gcacccacga cgcccgctac ctgatgatgg gccagacctt cttcgacgcc atcgtcgccc
     1801 actgccgcac cgacgccggc tacacgacgt tgaagagtgt ggtcacgatg gaaaaaggcg
     1861 acctgatgcc aagctacttc ctcgccgaga cgctgaagta cctctacctg ctgttcgcgc
     1921 cagactccgc gctcgatctc gacaaggtgg tgttcaacac ggaagcgcat cctttgaaaa
     1981 gagagctgaa gccttgaacg attcaaaaga tgacaagcaa ccattggcgg acgagctggg
     2041 cttgatgaaa tcgatcgtcg ctgccgcggt tgccgcggga tcgtcaacta cgagcaactc
     2101 gaatgagttt cgagcccgaa cgagcgatct tcagcaggta gagactctcc caaaaacaga
     2161 acggccgccc tctcgggcgg cctagccgga agctctcgct cctggcgcag tgtcgacgca
     2221 atgtaggatt ctcatcctat caatgtcaag tggcgagccg acaattccga cgcacaacac
     2281 ggtggtctac atcgacggat acaacctcta ccacggcttg cgagacaggc acggccgtaa
     2341 gtatctctgg cttgacctca ctcggtttgc tcgcgggttg ctctccgacc ccaggcagga
     2401 gctggtttca gtcaagtact tcacatcggc cgcgcccggg tcccgagatg gcgccaagcg
     2461 gcaacagatc tattggaagg cactggagac ctgcgcggga ctccagataa tcaagggccg
     2521 ataccgcgag aagcccacgc gttgctcagt atgcggaacg aaggaggcgg agtgtcgcat
     2581 ttgcggagca aagctgcgct tccggaacga aaagatgacg gacgtgaaca tcgcgacgca
     2641 tctcgtacga gacgcctgtt caggaacatt tgacgttgcg gtgttagtcg gaggagatac
     2701 cgacctcctg gccgctgttg aggcagcgaa ggctctgcgc cgaatcgtcg ttgcattccc
     2761 gccggatcgt gagaacgacg atatgaagcg cgcggcatca gggtggttcc gcttgagcga
     2821 aggcaaattc aagacggcac agttcccgga tgtcgtgaca gtcgctccgg gcatcaccgt
     2881 tacgcgaccc gttttgtgga ggtagcaaga ggtcctttca gctgagaacg actccggcac
     2941 gagacgcgca caacgcacat tcatgaacct cccctccatc cttacctggg gcctcgcggc
     3001 gacgatcgtg ctcacaaccc tttcgatcgc ggcgcaatcg ctcggcttca cgcggatcga
     3061 tataccgttc attgttggga cgatgttcac gcccaatcgc gaccgcgcca aagtcctcgg
     3121 gctcggcatg catatcatca acggctggat cttcgccctc gtgtacgcgt tcgcgttcga
     3181 gagccggcat atggcgtcgt ggtggtttgg cgggatgatc ggagctgttc aggggatgtt
     3241 catcgtgatc gtgctgctac cggtgctgcc gggattgcat ccgcggatgg tctcggattt
     3301 tcgcggcccc gagccgacgc gtttgctcga gccgccagga atcttcacca cgaactacgg
     3361 catcaagacg ccgttcgtgt tgctgttcgc gcacatggtt tatggcgtgg ttttggggac
     3421 gttttacacg ttgcggtgat tcacgttcca accgtttcac gcggagacgc ggaggcgcgg
     3481 ggtttggctt cgccctttcg ggatgcttct tgaagagcaa gcgcggcgtt gaggagcgcg
     3541 atatgcgtga gcgcctgcgg gaagttgccg agtaaggcgc ctgtgtggac gtcgacctcc
     3601 tcggaaaaca ggcccgcgtc gttggcgtgg cggactacgt cgtcaaagag tgcgtgggct
     3661 tcatcgacgc ggccggcgag ggccagcgct tcgactagcc agaaggtgca ggccacgaac
     3721 gcaccctcgc cggctggcag gccatcctcg gtcttgcggg tgtcgtagcg atagagcagg
     3781 ccgtcgccgc ccagggtgga gcgaatggcg tcgatggtgc cgagcatgcg cggatccttc
     3841 ggatcgatga ggcccacgcg ggcgataaag aggaggctgg cgtcgacctc cttctcatcg
     3901 aagatgccga cgaaggtgtt gcgctccgca tcgaagcccc gcgtgagaac ggcttccttg
     3961 atcgcggcct tcgtttgctc gagatgcgga cgaccgacct tgatgtggca acggtcggcg
     4021 atcttcaagg cactatcgag cgcggaccaa gccatcacct ttccgtgtac gtgctggcgc
     4081 gctccgccgc gcttctccca gatgccggcg tcgggctccc gccatcgttc gcagacgatc
     4141 tcggtcagac ggcgcagcag gcgttgcgag tcgcggtcga gagtcgcgcc gcgctgcgtg
     4201 gcctcatcga ccgctccgat cacctcaccg tagacgtcga gttgaaactg cgcgtgcgcg
     4261 gcgttgccga tccgcaccgg ccgcgaaccg cagtagccat cgagataatc gagcgtcttc
     4321 tccgggatgt gcgcctcgcc gaaaacgtcg tacaacatct gcagattcgg ctgcgtgagg
     4381 cgcgtcgagt agaggagcca gtcgagaaag gcctcgcctt cgttggagaa accgcagtcg
     4441 tagagcgcgc tgacggtcca cgatgcgtcg cgcagccagc agtaacgata gtcccagttc
     4501 cgctcggcgc cgatcgattc aggaagtgac gtcgtggccg cggcgacgat cgctccggac
     4561 ggcgtatagg tcagtaactt caacgcaagc gccgaacgaa ggacgagatc gcgatacggt
     4621 ccgtcgtacg tgagctggcc cgaccactcg cgccagaagc cgatcgtctt ctcgatctcc
     4681 gcggttgcct cgtcgccgat cggcgcgagc acgacggggg tgtgatcgtc gaagccgagc
     4741 gcgaatgtgc gcgactcgcc ggcgcgaagg ttgaagctcg atgtcgcggt gccgcgctcg
     4801 accaggaact ccgcatcgct gcgaagattg aggacgcgcg cgcgccatgc gcaggaaacg
     4861 gaatcgttgc gcaccttgag cgacggcgtg atgcggccgt agtcgggccg cggcgcgtag
     4921 gtgacggtga tcggaacctc tccgtcgacg cattcggcgc gccgcagcaa ttgccggaac
     4981 ggcgacagcc gcttttgctt ctccaactcg gtcatcaccg gcatcagatc gaggaggcgc
     5041 acgactccgg aagcggtggt gaacgtcgtc tcgatgacgt tcgtcccttc gaggtagcgg
     5101 cgtttcgact cgaatggaat cgcgggctgg atggcgaagt agccgccttt ctccgtatcg
     5161 agaattcgtc cgaagaccga ttccgaatcg aagcgcggcc agcagagcca gtcgatcgag
     5221 cccctgcgcg agagcagcgc gatggaacgc gtacagccga tggccgcgta atcggcgatc
     5281 ggaacgtatg gatcgatcac tttacctagc gtttccgcca tcgtcgcaac aaggccaaca
     5341 ccccggctgc gagagcgatg cctgcggcga ccccagccgc tccccaggcc actttcttga
     5401 tgtcggccgg cgccggctcg tcggcgtagc cgcgctccgg caacgcgccc ggcgcgttct
     5461 gcttcatcgc cagatggagc acctgcgcga ggtgcagcgc ttcgcgtccg gtgccctgcg
     5521 cgatctgctc gcggcaactg aaaccgtcgg cgacgatgat cgtgttgcgc gccgcttcgc
     5581 gcacagcagg cagcaggacg cgctcgccga cgcgcatcga cagctcgtac ttgtcgggct
     5641 cgaatccgaa cgcacccgcc atgccgcaac agccggagtc gggttgatcg acctcgagtc
     5701 ccatcttgcc gaggacctgc gcttcgtcgt cgaagtggag cacggccttg tgatggcagt
     5761 gcgcctgaac gagcgctttg ccctgtagct tgggcagctc gacgccctcc tgcagcagaa
     5821 actcgggcaa tgttttcaca gccttcgaca aacgcgttgc cacatcgttg cctggaaaga
     5881 gattcggaag ctcatcgcga aacacggaga tgcagctcgg ctcgaggccg acgatgggga
     5941 tgccggcgtc gagctcgggg cgcagcgcct ccatcgtctc gagcaggagc tttttggcca
     6001 tgccgaggaa accccagtcg tagagcgggc gtccgcagca gaggcgtttg cgcgggatga
     6061 tcacctcaaa cccggccgcc tccagcacct cggttgcggc gttcgcggtg ccgggatgga
     6121 agtgattgtt gaaggtgtcg ggccacaaga gaaccttgcg taggtcggac gaacgtaggt
     6181 cggacgaacg taggtcggac tctctagtcc gacgtttcgt cttgtcggac tgagagtccg
     6241 acctacgttt gaattgctcc acaaacgtgc gcttcgcaaa gtgaggcatc gtccgctccg
     6301 gcgcgatacc accaagtttc cgaaagaccg gcagcgacat gatcgcgttc gcgaggcccg
     6361 gcgcgagcga ggcgatctgt gcccaccagt agatccagcc catcgagtac gcagccaccg
     6421 gcctcaattt gcgttcgtag tagtgtgaaa ggaactccgc cttgtacgtg gccatgtcca
     6481 cgttgacggg acactcgccc ttgcagcctt tgcaggccag gcagagatcg agggaatcct
     6541 tgaccgcttc gttgttccaa tcggcgggga tgacctgccc ctcgaacatc tcaaacagga
     6601 gccgcgcgcg gccgcgcgtg gagtgctctt cctcgcgcgt gaccatgaag ctcggacaca
     6661 tcgtgccgcc atccagccgc cggcacttgc cgacgccgac gcagcgtccg accgcgctgt
     6721 cgatgcggcc gtgatcgtcg gggaactgga agtgcgtggc cggcgaccat tgtttgaagt
     6781 ccgcgccgag ccggagattc tcggtcgggt gatacggctt caccaccttg ccgggattca
     6841 tcttccactc cggatcccag agcgacttga actcgttgaa ggcctggatc aactcgggtc
     6901 caaacatctt cggcaagaga tcggcgcgag cttggccgtc gccatgctcg ccggatagcg
     6961 agccgttgta tttgatgcaa aggtcggccg cttcgtcgag ataggcgcgg aacttcgcga
     7021 tgcccgctgc gctctcgaga tcgaagttgt tgcgtgtgtg gacgcaggcc tggccgaagt
     7081 ggccgtagag atcgccgctg tatccgtact tgttgaggag cacgcgcagc tcgcgaaggt
     7141 aggcgccgag gtgtttggga tcgacggcgg aatcctccca accttcccat gtgtggccct
     7201 tgccgggaac gaacgcggtc gcgccgaggc cggactcgcg gattgcccag accagcttcg
     7261 catcgcggtc attgcagacg cggtgggcga tcttgagcgg cgacgcagcc agcgacgcga
     7321 tcatcgttcg tgcgtaggcg tccgaggctt ctttcgtcgc gccgccaaac tcgacgagca
     7381 gccaaccctt cccctcgggg agcagatgca gcttgtcgat gttgaggcct ttggcgcgca
     7441 tgtcttcgac gagacgatcg tcgatccctt ccagcccgat gggacgaaat tcgagcaggc
     7501 ggggcacatc gtcggcggcg aggtagatgt cgtcgtagcc gaggacgacg agggtgcgcg
     7561 agggaggcga atcgacgagg cggcacttcg cctcgagcac gaccacgcac gtgcactcgc
     7621 tgccgacaag cgcctgggcg acgttgaagc cattctcgga aagaagggcg tcgaggttgt
     7681 agccggaaac gcggcgcggg atcttcggat acttcgcgcg aatgaggggt ccgtacgtgt
     7741 cgcgaatcct cttcaatccc gcgtagatct ctccccgcct tcctccctcg gcgatgatct
     7801 gttcgagctc gctgtcgctc gtcgcgccga cccgcatgcg cacgccgtcg tacgtgagga
     7861 tgtcgagctc ctcgatgttg tcgacggtct tgccggccat cagcgcgtgt acgccgcagg
     7921 agttgttgcc gatcatcccg ccgagcgtgc aatacgcgtg ggtggccggg tctgggccga
     7981 aggtgagttt gtgcttctcc gcggcattgc ggagatcgtc gaggatgatg cccggctgca
     8041 cgcgcgcgaa ccgtgcctcc ggattcatct ccgggattgc gtgcatgtac ttcgagaagt
     8101 cgaggacgac agcgacgttg cagcattggc cggccagcga cgtcccaccg ccgcgcgcca
     8161 gaatcggcgc accatgcttg cgcgccagcg ccacggtggc gacgacatcg tgcgcatcgc
     8221 gcggcacgac gacaccgatc ggcacctggc gatagttgga ggcgtccgtc gcgtaaagcg
     8281 cgcgggcgcc atcatcgaaa cgaacttcac cgcgaacctc acgacgcagg tcggccgcga
     8341 gcaacgccgc atcgacgttc agcgtctgaa cacggtcgct gtgctcgatg cgcgtccgca
     8401 ccggccggtc atgctcgaac gcgaccggat cgaagcgcgt ccgctcgtcg cgatccttcg
     8461 gcgaagcggc agcgctgacc gcgaggtctt cggcgtgtac gtgcggcgtg tcggacacgg
     8521 gagacatggt attggttttc tgtcctctga cgacactcac gcgaagccgc gaagagatgc
     8581 aacgatgttc aaccagactt cgcgtcttcg cgtcttcgcg tgagactgtc aaacgccatt
     8641 accgcggcgg caggatcaga gtgacctcag acgtgtcgtt attcgtcacc gacgcaaatg
     8701 cccagaagta agtattggga tagatcgcct tgacgcgaat gcgcacgggc tccgcggctt
     8761 cctgcgtgcc cggatccaga gagaactgcc cataagcagg ggccggaacc gcaaacccac
     8821 cgacaggagg gttgagctgg acctgatcgg cttgaagaac atttccagat gcgtcaattt
     8881 gttcgacgct gagcggcagg gctttgtcga gcatgtagaa ccgcactaac acgcgaaacc
     8941 tcggatcgcg cggcacggcg atcaggttca tttcgtccgc acgaaactcg tcttcgcgaa
     9001 cgacgggaag gtttacgccg gcagacgacg cgttgcgagt gaggtcgcgc agcctcactt
     9061 cgagctggat tgaatccact gtggcaaagt atcggccttg gggacgattt tctgcatatc
     9121 ggatcgtgct ctgcgggcga atggtattga tctctgcgca gcaacaagcc tggttgcatg
     9181 ggttagtgct atagacgatc ggctggctgg aaacattcgt aatccacata tcggtctgcc
     9241 agaccgagcc cattgcaccg ggtaagttcg agactaatac cggaaccagg agccgggggt
     9301 tggctggcgc agttgcggca tcgatgggta ccgtacgccg tgaaatggac tgcgccgaga
     9361 caccactcga caacagcgaa aaggcaagcg cagcagacaa gaaatagttc acgtacgtct
     9421 cccgggaacg atatcaaata actgaaccgc tctcaggtag atttgttccc gggagtcggt
     9481 gatcgggagc cggagcggaa tgatcatttc aaactctccc actccccacc ccccacaaac
     9541 ccagcgtcaa cgcaacgaac ggattctcgg tcctacctgg cttcgcaccc atttcagcca
     9601 tggatcgtca acggaatgga gttgctcgtt cgttggccct aatgcatacc aatcaacggt
     9661 gtatgcacct gctaccatct tgagaaccag cagataacgg cccccttgac gataagtgtt
     9721 cgcgaagcag cttcctcctc tgccgccgcg gcgcacgaag gtatacggca ccggtagttc
     9781 gttgaaatcg tccgacgatt cgaggtaacc ggggagatcg agatcggtaa gcgcatatcg
     9841 ccccttcacg acttcctcga tccgaaaacg gacggtcgac tccggcctcc atgacgtgcg
     9901 accgaacgcc gtgggcatcc gactgtagtc gacggttgcg cgaacgatca ggtctgccga
     9961 cgccaccatg tcctcgggga ccacaggacc gaagcgctgg caagcggaga gaaccccggt
    10021 tcggagagcg accacgctga cgacaatgat cgcgtaggcg aagactctct tgatcgaagc
    10081 ttgcatctcg ttattctgga acggacgagg caaacttggc gaaatctgcg cacatctgcg
    10141 tccatctgcg gataaaaact gtatccgcag atgtcgcaga tgatacgcag ataaagactt
    10201 agaacttcac gccgacgatt actcttcgtc ccggcgacgc gtagcccacg acctcctgat
    10261 acgacgagtt ctg
//
LOCUS       DATLVW010000888         6080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_82235, whole genome shotgun sequence.
ACCESSION   DATLVW010000888 DATLVW010000000
VERSION     DATLVW010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82235"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1510
                     /locus_tag="VNN25_26305"
     CDS             179..1510
                     /locus_tag="VNN25_26305"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HXH95114.1"
                     /translation="MSDIAVAEKQWFGHPRGLATLFFTEMWERFSYYGMRALLVLYMT
                     GSVMQPGLGFPDAKATNIYGLYTMLVYLMGIPGGFIADRFLGHYRAVFVGGVIIASGH
                     FTMAIPGIPFFYTGLLLIILGTGLLKPNVSTIVGTLYTRDDPRRDAGFSLFYMGINLG
                     AFIAPLVTGWLGQKINWHIGFAAAGVGMVLGLIQYVYGRKYLIARSGPEKPVVAAIAA
                     TPVVQEKAFTKQEWGRIVAALILTLFALIFWAGFEQAGSSLTLFADRATRLTILGFNY
                     PSSWFQSVEPLFVIIFSPIFAWIWFTMSARRHEPSSPAKFTLGLFFLTFSFLLMVPAA
                     RYFQSTGSRVSPLWLVGLYFLQMAGELCLSPVGLSMVTKLSPPRVVGLMMGFWFFATA
                     MGNYVAGWVAGLVGHRTYSEVFMIAAVTAAVTTLILAVMIPIIKRLMAGVN"
     gene            complement(1543..2301)
                     /locus_tag="VNN25_26310"
     CDS             complement(1543..2301)
                     /locus_tag="VNN25_26310"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXH95115.1"
                     /translation="MPKIIFSSDENAYRITLADPPLHILDITMLEELRDAIARVAPDR
                     HVLIIDSQGEKAFSAGASVQDHLGDRVATMLSHFHDCFRMLAKLDLVTVALVRGAALG
                     GGCELALACDFVLASERAKFSQPEINLGVFPPVAAYQLSRQLSSRKGLELLLTGDSID
                     AAAADRLGLVNAVFPLAEFDARADAWLQRLYKQSASSLRFARRAFRIAQSADFYERLG
                     AVERLYLDELMETHDANEGLNAFVEKRKPEWTGH"
     gene            complement(2311..5034)
                     /locus_tag="VNN25_26315"
     CDS             complement(2311..5034)
                     /locus_tag="VNN25_26315"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95116.1"
                     /translation="MFGRVAVAVSLLLVSFAAFPQCNLTSVASVPFRASYLDIAIDGN
                     DLWAATSYGVSLFDRSVDPPALVVAFPLPGVTRVVRATNGLAYVGSGSSLYVVRKNGR
                     NLQLLRTIDAGATVNDLLLTTLDLYVATTAGLTQWDLLDPTTPSKTPAIFTTSTPNVR
                     SLAISGSTLFAADGDASVEAFNISVPSIPNGIGTITSLPGSVAVGAANGRLYVSDGFT
                     TDIFDSAEGGGSSAIKLARDSFGAVAVAPINTTAIFAAGADNRVRAFDLAASASPVEL
                     FRAELFPAGGTVNRIGRIAAASNRLYVAGGDLGLLTYDTTSFATPFPLRSYATGATTS
                     VVSLATSTGINVYVSRAATGITEFSQTSVGALTQLRSWDSNNDTVWDGGNNFLLTSSG
                     ATASFWALSSATPTLVSTLTLRAPIASAVLIGTTGYFVLNETTGGTLWSADLAPATPT
                     AQQITLTAIKPSFIARSGSALILAELKTDGTTSLSYFAAADFTKAPVTTNIAGLATGG
                     VALSGSTAAISTFSGVTLADFASGSIATLPQSNGAIARRLAFAGTTLVELTDNALLFW
                     NTQTRTLTKQFTLPAVPVSVHVGPDNAVADVATEDGITTVQLAATTRVPSLLSTSNGN
                     VYYKKLVAGSQRLYFFDGHNVDVFTNTMRYTGGIRTPLVVDVAASDSAVFTIQNNLAV
                     TAYGREGNALTTSAINEGTDAQAQSIASAGGTAWASIVLNCHSSGCEGKTIVFDPKSA
                     FAQTATMTGTVRDVVTSGNRAYVLTELPNEVRVVDITDPAHPVVLYTHATEGAQPPSS
                     IAFANGTVYVLGEKLYTYAAGDLSKTGEQLSSAVFDPATQKIRADGACVAYTGREFSP
                     QFLGGTPVVTSPATARFVASQPGTLYFLTDYSLEIWSAKPLPQPARRRPSR"
     gene            complement(5053..5196)
                     /locus_tag="VNN25_26320"
     CDS             complement(5053..5196)
                     /locus_tag="VNN25_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HXH95117.1"
                     /translation="MLETIHVFKSPNNARRLLDAFEQSTGGEGKPQTVEQLRAELGLD
                     ESS"
     gene            complement(5216..5788)
                     /locus_tag="VNN25_26325"
     CDS             complement(5216..5788)
                     /locus_tag="VNN25_26325"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXH95118.1"
                     /translation="MRFTTALAVLAVATTLHADSRTYTIAKGGNNVAEFHAEDTYDSF
                     DGKTSDVTGTIVADPANPASSSVQVSINIDSLDTGVSLRNKEMRERYLQTDKFGTATF
                     KSVSVTGPASIAPNQPAEISVTGDMTLHGVTKRMTIPVRVVLIPEGRIHATSNFKIHM
                     PDFGINVPHTIIVTVNDEVPVRLDVWAVGK"
     gene            complement(5809..>6080)
                     /locus_tag="VNN25_26330"
     CDS             complement(5809..>6080)
                     /locus_tag="VNN25_26330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95119.1"
                     /translation="GQLMRGMFHLPHGDTTLLTGAPLRVGIVETLGTFRHTVTNRRIT
                     FEVFAVTRRLRTRGREYAWIDPKLLAATPHPSYVRKALQLAGIGQ"
BASE COUNT         1092 a         1810 c         1981 g         1197 t
ORIGIN      
        1 cttcgcggat ctcatccacc gtctccgcac ggacgaaaag ccgcgccggc ccaccgattc
       61 cgagggtcgt cagcggggcg agaggaacat tccgctccag ccggcgcggt gcaacactct
      121 gcaacgactc cgtcatcgcc gttgcattgt gcatcatcgc tgcctgagaa tggccggcat
      181 gtcagacatt gccgtcgcag aaaagcagtg gttcggccat ccacgtggat tggcaacgct
      241 cttcttcacc gaaatgtggg agcgcttcag ctattacggg atgcgcgcgc tgctcgtgct
      301 ttacatgact ggttccgtca tgcagcccgg cctcggattt ccggacgcaa aggcgaccaa
      361 catctacggc ctctacacga tgctggttta cctgatggga atcccgggcg gtttcatcgc
      421 cgatcgtttt ctcggccact accgcgcagt ctttgtcggc ggagtcatca tcgccagcgg
      481 ccatttcacg atggcgattc ctggcattcc gttcttctac accggcctcc tgctgatcat
      541 cctcggaacg ggcctgctca aaccgaacgt cagcacgatc gtcggaacgc tctacacgcg
      601 cgacgatcca cgccgtgacg ccggcttctc gctgttctac atgggaatca acctcggtgc
      661 gttcatcgct cccctggtca ccggctggct tggacagaag atcaactggc acatcggctt
      721 tgcagccgcc ggcgtcggca tggtgctggg cctgattcag tacgtctacg gccgcaagta
      781 cctcatcgcc cgttcgggac cggaaaaacc ggtggtggca gcgatcgcgg cgacgccggt
      841 ggttcaggaa aaggcattta cgaagcagga gtggggccgc atcgtcgccg cactgatcct
      901 caccctcttt gcgctgattt tctgggccgg tttcgagcag gctggttcga gcctcactct
      961 cttcgcagac cgcgcgacgc ggttgacgat cctgggcttc aactacccat cgagctggtt
     1021 ccagtcggtg gaaccgttgt tcgtcatcat cttttcgcca atcttcgcct ggatctggtt
     1081 cacgatgtcc gcacgccgcc acgagccatc gagtcccgcc aagttcacgc tcggtctctt
     1141 ttttctcacg ttctcgttct tgctgatggt tcccgcagcg cggtacttcc aatctaccgg
     1201 ttcgcgcgtc agcccgctgt ggctcgttgg tctgtacttc ctgcagatgg ccggcgagct
     1261 ctgtctcagc ccggtcggtc tcagcatggt gacgaagctg tctccaccga gggtcgtcgg
     1321 cttgatgatg gggttctggt tcttcgccac ggcgatgggc aactacgtag cggggtgggt
     1381 ggcaggtctg gtcggccacc gcacctactc cgaggtattc atgattgcgg cggtcactgc
     1441 cgccgtcacg acgttgatcc ttgctgtgat gattccgatc atcaagcggt taatggcagg
     1501 agtgaactga acttcgggga tgcctcattg aacgggactg aatcagtgtc ccgtccactc
     1561 cggctttcgc ttttcgacga acgcattcag tccttcgttg gcgtcgtgcg tctccattag
     1621 ctcgtcgaga tagagacgct cgacggcacc gagccgctcg tagaagtcgg cgctctgggc
     1681 gattcggaac gcccggcgcg cgaaacgcag cgacgacgcg ctctgcttgt agagacgctg
     1741 cagccatgcg tcggcacgcg cgtcaaactc ggcgagcgga aagacagcgt tgacgaggcc
     1801 gaggcgatcg gcggccgcgg catcgatcga gtcgcccgtc aaaagaagct cgagcccttt
     1861 gcgcgacgac agttgcctcg agagttggta agcagcgacc ggaggaaaga cgccgaggtt
     1921 gatctcgggc tggctgaact tggcgcgttc ggaggccaga acgaagtcgc acgccagcgc
     1981 gagctcgcat ccgccgccga gcgccgcgcc gcgcacgagc gcgaccgtaa ctaggtcgag
     2041 cttcgccagc atgcgaaagc aatcgtggaa gtggctgagc atcgtggcca cacggtcgcc
     2101 gaggtgatcc tgcacgctgg cgccggcgga aaaggctttt tcgccctgcg agtcgatgat
     2161 cagaacgtgc cggtcgggtg cgacgcgtgc gattgcatcg cgaagctctt cgagcatggt
     2221 gatgtcgagg atgtggagtg gcggatcggc cagagtgatg cggtacgcgt tctcgtcgga
     2281 cgagaagatg atcttcggca tttgcagatg ctatcgcgac gggcgccggc gcgccggttg
     2341 cggcagcggc ttggccgacc agatctcgag cgagtagtcg gtcaggaaat agagcgtgcc
     2401 cggttgcgac gcgacaaaac gggcagtggc aggcgaggtc acgaccggcg tcccaccgag
     2461 gaattggggc gagaattcgc ggccggtgta ggctacgcat gctccgtcgg cgcgaatctt
     2521 ctgcgtggct ggatcgaaga cggcggacga gagttgctct cccgtcttcg agagatcgcc
     2581 cgccgcgtag gtgtagagct tctcgccgag aacgtaaacg gtgccgttgg cgaaggcgat
     2641 cgatgagggc ggctgtgcgc cctccgtcgc gtgggtgtac agaaccaccg gatgcgcagg
     2701 gtcggtgata tcgacgacgc gcacctcgtt cggcagttcg gtgaggacgt aggcgcggtt
     2761 gccgctggtc acgacgtcac gcacggtgcc ggtcatcgtc gcggtctgtg caaaggcgct
     2821 cttcggatcg aagacgatcg tctttccctc acaaccgctg ctgtggcaat tcaatacgat
     2881 cgaagcccat gccgtgccgc cggctgaggc gatcgactgc gcctgcgcgt cggttccttc
     2941 gttgatcgcc gaggtcgtga gcgcgtttcc ttcgcggccg taggccgtga ctgccaggtt
     3001 gttctggatc gtgaagacag cgctgtcgct cgcggcaacg tcgacgacga gcggtgtgcg
     3061 gatgccgccg gtgtaccgca tcgtgttcgt gaagacgtcg acattgtgcc cgtcgaagaa
     3121 gtagagccgc tggcttccgg cgacgagctt cttgtaatag acgttgccgt tcgacgtact
     3181 gagcagcgat gggacgcgcg tcgtcgctgc gagctgcacg gtcgtgatgc catcctcggt
     3241 cgctacatca gccacagcgt tgtcagggcc gacgtgcaca gaaacgggaa cggcgggcag
     3301 cgtgaattgc ttcgtcagcg ttcgtgtctg cgtgttccag aacaggaggg cgttgtcggt
     3361 cagctccacg agcgtcgttc ccgcaaaagc cagccggcgc gcgattgctc cattcgattg
     3421 cggcagtgtt gcgatcgagc cgctcgcgaa atcggcgagc gtcacgccgc tgaacgtcga
     3481 tatggctgcg gtcgagccgg agagtgcgac gccgccagtc gccagtccgg cgatgttcgt
     3541 ggtgacgggc gccttcgtga aatccgccgc ggcaaagtat gagagcgagg tcgtgccgtc
     3601 cgtcttcaac tcggccagga tcagcgcgga gccggaacgc gcgatgaacg acggcttgat
     3661 cgccgtgagc gtgatctgct gtgccgttgg agtcgccggc gcgagatccg cggaccagag
     3721 cgttcctccg gtagtttcat tgagaacaaa atagccagtc gtgccgatca gcaccgccga
     3781 ggcgatcggc gcgcgaagag tgagagtgga gacgagcgtc ggcgtcgctg agctcaaggc
     3841 ccagaaagag gcggtcgctc cggatgatgt caggagaaaa ttgttgccgc cgtcccagac
     3901 cgtatcgttg ttgctatccc acgaacgaag ctgcgtcagt gcaccgacgg acgtctggct
     3961 gaactcggtg atgccggtag cggcgcgcga aacgtaaaca ttgatgcctg tgctcgtcgc
     4021 aagcgagacg accgaagtcg ttgcgccggt tgcatagctg cgcagcggga acggtgtcgc
     4081 gaacgatgtc gtgtcgtagg tcagcaggcc gaggtcgccg ccggcaacgt agaggcgatt
     4141 ggaagcggca gcgatccgtc cgatgcgatt gaccgtgcct cctgcgggaa agagctcggc
     4201 gcgaaacaac tccacagggc tcgcggaagc ggcaagatcg aaggcgcgga ctcgattgtc
     4261 ggcgcccgct gcgaagatcg cggtggtgtt gatcggtgcg acagccacgg cgccgaacga
     4321 atcacgcgcc agtttgattg cactgctgcc gccgccttcg gcgctgtcga agatgtccgt
     4381 cgtgaagccg tcagagacat acaggcggcc gtttgctgcg ccgacggcga ccgagcccgg
     4441 aagcgatgtg atcgtgccga tcccgttcgg aatcgacggc acgctgatgt tgaacgcctc
     4501 gacggacgcg tcgccgtccg cggcaaacag cgtcgaaccg ctgatcgcga gcgagcgcac
     4561 gttcggggtg ctggtcgtga agattgccgg cgttttggac ggcgtggtcg ggtcgagcag
     4621 atcccattgc gtaaggccgg cggtggtggc cacgtagaga tcgagcgtcg tcaacagcag
     4681 gtcgttgacc gtcgccccag cgtcgatcgt tcgcagcagt tgcaggttcc gtccattttt
     4741 cctgaccacg tacagagaag acccgctgcc gacgtaggcc agaccattcg tagcgcggac
     4801 cacgcgcgta acgcccggca gaggaaacgc gacgaccagc gcgggcggat cgacgctccg
     4861 atcaaagaga gagacgccat acgaggttgc cgcccagagg tcgttcccat cgatggcgat
     4921 gtcgagatac gaagcgcgaa acgggacgct ggcaaccgac gtgagattgc actgcggaaa
     4981 ggctgcgaat gatacgagga gaagtgagac cgcgaccgcc acacgcccga acatgagagg
     5041 gattctaggc cactagctgc tctcgtccaa ccccaactcc gcgcgcagtt gctcgaccgt
     5101 ctgcggcttt ccttcgccac ccgtagattg ctcgaatgcg tccagcaatc gtcttgcgtt
     5161 gttgggcgac ttgaagacgt gaatggtttc caacaaaccg cgaaagtcgt atgtctcatt
     5221 tccccaccgc ccacacatcc aacctcaccg gaacctcgtc gtttaccgtc acgatgatcg
     5281 tgtgcgggac gttgatgccg aagtcgggca tgtggatctt gaagttgctc gtcgcgtgga
     5341 tgcggccttc cgggatgagg acgacccgca cgggaatcgt catccgcttc gtcactccgt
     5401 gcagcgtcat gtcgccagta acgctgatct cggctggctg gttcggcgcg atcgacgccg
     5461 ggccggtgac gctgaccgac ttgaatgttg cggtgccaaa cttgtccgtc tggagatagc
     5521 gctcgcgcat ctctttgttg cgcaggctta ctccagtgtc gagcgagtcg atgttgatgc
     5581 tgacttggac gctcgacgag gcagggttgg ccggatcggc gacgatcgtg ccggtcacgt
     5641 cgctcgtctt tccgtcgaac gagtcgtacg tatcttcggc gtgaaactcg gcgacgttgt
     5701 tgccgccctt cgcgatcgtg taggtgcgcg agtcggcgtg gagagttgtg gcgaccgcaa
     5761 gcactgccag cgcagttgtg aatctcatgc atccgcgaac gcgagcgatt actggccgat
     5821 tccggcgagc tgcaacgctt tgcggacgta ggacgggtgc ggagttgctg cgagcagttt
     5881 gggatcgatc caggcgtact cgcgcccgcg cgttcgaaga cgccgtgtca ccgcgaagac
     5941 ctcgaacgtg atcctgcggt tcgtcaccgt gtggcgaaac gtgccaagcg tttccacgat
     6001 cccgacgcgc agcggtgcgc cggtcagaag tgtcgtatcg ccgtgcggca gatgaaacat
     6061 cccgcgcatc agttggcccg
//
LOCUS       DATLVW010000889         5096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_407537, whole genome shotgun sequence.
ACCESSION   DATLVW010000889 DATLVW010000000
VERSION     DATLVW010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407537"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VNN25_26335"
     CDS             <1..385
                     /locus_tag="VNN25_26335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH95120.1"
                     /translation="NWIRRLKDLGCLIALDDFGAGFSSFGYLQALAVDYIKIDRTFVH
                     DLDTNATNRALVQAVNTVARTLGKEVIAEGVECEAHADALRAIGIEHGQGFHWGIPEE
                     LEATLLGMTKVSGYASDRAAQLVSR"
     gene            360..785
                     /locus_tag="VNN25_26340"
     CDS             360..785
                     /locus_tag="VNN25_26340"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HXH95121.1"
                     /translation="MPLSLFHDKNIPPSDDALRSALAGTAGLWRELIEQLGVDFAPLA
                     FVWGFTSKSTGWSLRVKRGERNILYMTPCEGHFLVSIGLGERAAAAARKSELREAILP
                     LIEAAPKYAEGRGIRFEVHGGQPEKVAVAVVLAGLKIAN"
     gene            1503..1895
                     /locus_tag="VNN25_26345"
     CDS             1503..1895
                     /locus_tag="VNN25_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXH95122.1"
                     /translation="MDREWNDSQPIYRQLRDRVVAMILDGMLKEGDPLPSVRTVAAEY
                     RVNPITVLKGYQQLVDEQLVETKRGRGMFINAGARDLLMQGERQKFLAEQWPRIHATI
                     QRLGLTPEELLDATTIETSKVEEETPKS"
     gene            1892..2755
                     /locus_tag="VNN25_26350"
     CDS             1892..2755
                     /locus_tag="VNN25_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH95123.1"
                     /translation="MTAIEARGLRKTFGSTIALDSIDLRVEEGRILGIIGPNGAGKTT
                     ALNAILGLTSYEGELTVLGRNPWTERDLLMRDVSFIADVAVLPRWMRVSQILDYVSGV
                     HPRFRRAKAEAFLTKTTIKRSSKIRELSKGMVTQLHLALVMAIDAKLLVLDEPTLGLD
                     LLYRKQFYDSLLNDYFDRTRTIVVTTHQVEEIQDVLTDLMFIDRGRIALTCSMEEFES
                     RYLEVMVLPEHVAAARALGPLHERQVLGRSILLFDHADRQQLAALGDVRTPGIADLFV
                     AVMGNQPGEIR"
     gene            2752..3666
                     /locus_tag="VNN25_26355"
     CDS             2752..3666
                     /locus_tag="VNN25_26355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH95124.1"
                     /translation="MSEQSSAVPEFAAASPVVVQAPRPMYWSVRRELWENRSIYVAPL
                     LVAAVVMFGFVLSTIGMPHRRRATLLLAPAQQHTTIAMPFDIAAAMLVVTAFVIGIFY
                     CLDALQGERRDRSILFWKSLPVSDRTTVLAKTSIPLLALPAFTFVIIVLTQWFMLLWS
                     TAVLLPSGLAGSTWMHFHWFTQSLLVFYCLIVLVLWHAPIYALLLLISSWARRGSYLW
                     AVLPPLAIAAVEKIVFNTAYFGSFLKYRLIGGIGEAFVFKAPHGTDSLSRLTPLNFLS
                     TPGLWLGLILAAIFITAAVRLRRDREPV"
     gene            3691..4971
                     /locus_tag="VNN25_26360"
     CDS             3691..4971
                     /locus_tag="VNN25_26360"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HXH95125.1"
                     /translation="MSAEFPLTFHSADASRTRYSLRIIAANALKAAARFWFIVTVAGQ
                     LLFAFAVASFYGLSALRGDFHRWGKSITNGHVPGDTMGNLVVELHLLSAVIIILAGAL
                     QLIPVVRQRFPRFHRWNGRIYMLAAVTVSGAGLDMLWIRGSVADFPQRIGFTGNAILI
                     WLCASMALRFAIARDFTRHRTWALRLFLVVAASWFLRVELFLSFLIFRGPFGFDPLSF
                     SGPFLTFLSYAQYLLPLAVLELYLYAQRGAGSLRRMATAFVLFVMTVGTAAATFALTM
                     AVWVPEVKAGFDTRTSITETLSATIASGGIDQAIRQYHQLKAAKAAAYNFDQGELNIL
                     GYELLRKKHFSDAIRIFQLNVEAYPQASNVYDSLAEAYADTLNVPLTVANYQKALQLN
                     PKNRTATAGLKKLAAYGAAHAGSPGGSQAEGASQ"
BASE COUNT          915 a         1681 c         1501 g          999 t
ORIGIN      
        1 aaactggatc cgccgtctga aggacctcgg ctgtctgatc gcgctcgacg atttcggcgc
       61 tgggttctcg tcctttggct atctgcaggc gctcgcggtt gactacatca agatcgaccg
      121 caccttcgtt cacgacctcg ataccaacgc caccaatcgc gcgctcgtgc aggcggtcaa
      181 caccgtcgcg cgcacgctcg gcaaggaagt catcgccgag ggtgtcgagt gtgaagcgca
      241 tgcggacgcc ctccgcgcca tcggaatcga gcacggccag gggttccact gggggattcc
      301 ggaggagctc gaagcgacgc tgctcggcat gaccaaagtg agcgggtatg cttctgaccg
      361 tgccgctcag cttgtttcac gataagaaca tcccgccttc cgacgacgcg ctgcgctcgg
      421 cgctcgccgg cacggctggc ttgtggcggg aactgatcga acagcttggg gtggacttcg
      481 cgccgttggc attcgtctgg ggcttcacca gcaagagcac tggatggtcg cttcgcgtca
      541 aacgcggcga acgcaacatt ctttatatga ctccgtgcga ggggcacttt ctggtgtcga
      601 tcggactcgg cgagcgagcg gcggcagcgg cccggaagag cgaactgcgc gaggcgatcc
      661 tccccctcat cgaggcagcg ccgaagtacg ccgaggggcg gggcatccgc tttgaggtac
      721 acggcggtca gccggagaag gtcgcggtcg ccgtggtcct ggctgggttg aagatcgcga
      781 actgactcgc tgactggttg gcacctggga ctcggcgtca cgctggcggc catcagcgat
      841 cgaaggtctg gcccccactc aagaaagcca ccattcggga acagcgcaaa ctctcggtat
      901 tcgcgcctga gtcgaaccac cggggcgggg acgccccgat ggccgcgagc gagacgctcg
      961 cactccgccg aaaggagtgc ggacgtctcg tccgcgggca ctcgggcgtc ctcgcccgag
     1021 tgctgtcggg cgggggcaga ggtccgttga ccgctccggc gttgcctgaa atacgtagcc
     1081 ggtgcgcaac ctcttggctc gcgcgaagat ccagctaact cgctgactga atgaaatgtg
     1141 ttgctgggcg acggccgcgt gagcgtgaac gactgaatgt ctggccccca ccccgaacag
     1201 cgcaaactgt cggtatcagg ccgagtcgaa ccaccgggcg ggacgcccgg atggccgcga
     1261 gcgagacgct cgcactcctc aggtcgctcg ccggcacatc agttcgagcc aaattactga
     1321 ctccgcgatg cttgcaacct gccatcgacg ctgacgagga gtgccggact cgcctctgaa
     1381 gcacacgtcc aaaggtctga ccccgccttt tcatgaaaca aatgtcttgc atgtgcgtta
     1441 gtgttgtagt caactacatc actagatagc ggcgcacacg gcgcagcgac gagaaaaacc
     1501 aaatggatcg cgagtggaac gacagtcagc cgatttaccg ccagcttcgc gaccgcgtcg
     1561 tcgcgatgat cctcgacggg atgctcaagg aaggcgatcc gctcccttcc gtccgcaccg
     1621 tcgccgccga atatcgagtc aatccgatca ccgttctgaa gggctatcaa caactggtcg
     1681 acgagcaact cgtcgagacg aaacgaggcc gcggcatgtt catcaacgcc ggcgcgcgcg
     1741 atctgctgat gcagggcgag cgtcagaagt ttcttgcgga gcagtggcct cgcatccacg
     1801 caaccatcca gcgcctcggt ttgacccccg aagagctgct ggacgccacg acgatcgaga
     1861 catcgaaggt ggaagaggag acgcctaaat catgacagcc atcgaagcac gcgggctccg
     1921 caagacgttc ggttccacga tcgcgctcga cagcatcgat cttcgcgtcg aagaaggccg
     1981 catcctcggc atcatcggtc ccaacggcgc aggcaagacc accgcgctca acgccatcct
     2041 cggcctcacc tcgtacgaag gcgaactcac ggtgctcgga cgaaatccct ggaccgagcg
     2101 cgatctgctc atgcgcgacg tctccttcat tgccgacgtc gccgtgctcc cgcgctggat
     2161 gcgcgtctcc cagatcctcg attacgtatc cggcgtgcac cctcgttttc gccgcgccaa
     2221 ggcggaagcg tttctgacga agacgacgat caagcgcagc agcaagatcc gggagttgtc
     2281 gaaaggcatg gtcacccagc ttcatctcgc gctggtcatg gccatcgacg cgaagctgct
     2341 ggtgctcgac gagccgacgc tcggcctcga ccttctctat cgcaaacagt tctacgactc
     2401 gctgctcaac gactacttcg accgcacgcg cacgatcgtc gtgacgacgc atcaggtgga
     2461 ggagattcag gacgtcctca cagacctgat gttcatcgac cgcggccgca tcgccctcac
     2521 atgcagcatg gaagagttcg agtcgcgcta tctcgaagtg atggtgctgc ccgagcacgt
     2581 cgccgccgca cgggcactcg gaccgctgca cgagcgccaa gtgttggggc gcagcatcct
     2641 cctcttcgat cacgccgatc gccagcaatt ggccgccctc ggcgacgtgc gcacgcccgg
     2701 catcgccgac ctcttcgttg ccgtgatggg caatcagccg ggagagattc gatgagcgag
     2761 caatcgagcg ccgttcccga attcgcagcc gcttcgccgg tggtcgtgca ggcgccgcgg
     2821 ccgatgtact ggtcggtgcg ccgcgaacta tgggagaacc gctcgatcta tgtcgcgccg
     2881 ctgctggtcg cggcagtcgt gatgttcgga ttcgtcctca gcacgatcgg gatgccgcac
     2941 cgcagacgag ctacgctctt gctcgccccg gcacaacagc acaccaccat cgcgatgccc
     3001 ttcgacatcg ccgcggccat gctggtcgtc acggcgttcg tcatcggcat cttctattgt
     3061 ctcgacgcac tgcagggcga gcgccgcgac cgcagcatcc tcttctggaa gtcgctgccg
     3121 gtctcggacc ggaccactgt tcttgccaaa acgagcatcc cgctgctggc tctgcccgcg
     3181 ttcaccttcg tcatcatcgt cctcacgcag tggttcatgc tcttgtggag caccgcggtc
     3241 ctgctgccga gcggcctggc cggttctacc tggatgcatt tccactggtt cacgcagtcg
     3301 ctgcttgtct tctattgcct gatcgtgctc gttctctggc acgccccgat ttacgccttg
     3361 ctcctgctga tctcgagctg ggcgcggcgt gggtcctatc tctgggcggt gcttccgcct
     3421 cttgcgatcg ccgccgtcga gaagatcgtc ttcaacactg cgtatttcgg ttcgttcctg
     3481 aagtaccggc tcattggcgg aatcggcgag gccttcgttt tcaaggcgcc gcacggcact
     3541 gattcgctat cacgcctcac gcccctcaat ttcctgagca cacccggtct atggctcggg
     3601 ctgattctcg cagcgatctt catcactgca gcagtgcggc tgcgccgcga tcgggaaccg
     3661 gtctaacccg accggcgaag gagacaccgc atgagcgccg aatttccatt gacctttcat
     3721 tccgccgacg catcgcggac gcggtattcg ctgcgcatca ttgccgcgaa tgccttgaag
     3781 gcggcggcgc ggttctggtt cattgtgacg gtcgccggcc aattgctgtt cgcgtttgcc
     3841 gtggcgtcgt tctatggcct ttcggcactg cgcggcgact ttcaccgctg gggaaaatcg
     3901 atcaccaacg gccacgtacc gggcgacacg atgggcaacc tggttgtcga gctccacctc
     3961 ctctcggcag tcatcatcat tctcgccgga gcgctgcagc tcatccccgt cgtccggcaa
     4021 cgcttcccgc gctttcaccg ctggaacgga cgcatctaca tgcttgccgc ggtcaccgtc
     4081 agcggggccg ggcttgacat gctctggatt cgaggcagcg ttgccgactt cccgcagcgc
     4141 atcggattca ctggcaatgc gattctgatc tggctctgcg cgtcgatggc cctgcgtttc
     4201 gcaatcgccc gcgacttcac gagacatcgc acctgggcgc tcaggctctt tctcgtggtc
     4261 gccgcatcgt ggttcctgcg cgtggagttg ttcctctctt tcctcatctt tcgaggcccc
     4321 ttcggattcg atcccctttc cttctccggc cctttcctca cgttcctctc gtacgcgcag
     4381 taccttcttc ctctggccgt tctggagctc tatctctatg cacagcgtgg cgccggttca
     4441 ctgcggcgga tggccaccgc cttcgttctc tttgtcatga cagtcgggac ggctgcggcg
     4501 actttcgcgt tgactatggc cgtgtgggtg cccgaggtca aagcaggctt cgacacgcgg
     4561 acatcgatta ccgagacgct ctccgcaacc atcgcctcgg gcggcatcga ccaggccatc
     4621 cggcaatacc atcaattgaa ggccgctaaa gcggcggcct acaacttcga ccagggtgag
     4681 ctcaacatac tcggctacga actgctccgg aagaaacatt tcagcgacgc gattcgcatc
     4741 ttccagctca acgtcgaagc ctatccgcaa gcgagcaacg tgtacgacag cctcgctgag
     4801 gcatacgccg atacgctcaa cgtgcctctg accgtcgcca attaccaaaa ggccctccaa
     4861 ctcaatccga agaaccgcac tgccacggcc gggctgaaga agctggcggc ttacggggca
     4921 gcacacgcag gatcgccggg cgggagccag gccgaaggag cgtcccaatg agcattcttc
     4981 cgaacgccat gggcgacgct gcggaaactc cgccgccagc aatggcgcca actatgtccc
     5041 cgacaagttc cttttactgg tcggtgcgcc gtgaattgtg ggagaaccgc tcgatc
//
LOCUS       DATLVW010000890         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_326745, whole genome shotgun sequence.
ACCESSION   DATLVW010000890 DATLVW010000000
VERSION     DATLVW010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326745"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..618
                     /locus_tag="VNN25_26365"
     CDS             109..618
                     /locus_tag="VNN25_26365"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXH95126.1"
                     /translation="MNDIDGPLALKPFYRRNLPHIVNARPLFLTFNTLNRWILPPGAR
                     TLALRHALHEHREKIFMDVAVIMPDHAHLIFSLLQDKFEVPYSLADVMKGIKGVSSRR
                     INQLLGRTGSFVRQDESFDHVVRQSERSLAKYEYVCNNPVRAGLVKHADEYPWLWRSW
                     IEGRGSEMA"
     gene            complement(748..1848)
                     /locus_tag="VNN25_26370"
     CDS             complement(748..1848)
                     /locus_tag="VNN25_26370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH95127.1"
                     /translation="MSCLLIVDDEPVIGIGLAAALECDGRRIVVCRDLESAQIILENE
                     PVTDILTDVKLSGSFRFEGLDFITEVIRVSPATRVILMTGHASDELVAEAERRGADAV
                     LSKPFEIDSLLPLLTPPSDATPSSLTMVPTLDEVIGSGQLRPAFQPIVSLADGTTHGV
                     ESLARYRGGEPFVRPDLLFAYATRKHDVAALEAACIEATMRAVPSLPPSSLIFMNVHP
                     AALEELGVVIALESNAHRFGIDLGRVVLEITEQQEVKQTAALFETIEHLRTLGVRFAF
                     DDVGVAYSHLPLIERIRPEYLKISQLFGTRFEADPTLTKIVRNLVALAAEFSCAIIIE
                     GVEDASTADAARDLGIAYGQGWLFGRPEDAPG"
     gene            complement(1947..2090)
                     /locus_tag="VNN25_26375"
     CDS             complement(1947..2090)
                     /locus_tag="VNN25_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95128.1"
                     /translation="MSKINGDKARTAIEKRRRTAQRAKDRANLAGIKSAAVHPAAKKK
                     NAK"
     gene            complement(2135..>2622)
                     /locus_tag="VNN25_26380"
     CDS             complement(2135..>2622)
                     /locus_tag="VNN25_26380"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXH95129.1"
                     /translation="AFAADAPQPPAEGTAAPKFSLQSQEAANVSLDQFKGKWVVLYFY
                     PKDFTSGCTLEAHNFQRDIAKYEAKNAVVVGVSLDPVDSHKGFCTKEGLSFKLLADTS
                     HTVSSSYGSVMDHDGTMYAARNTFLIDPSGVIRKVYLKVKPATHSDDVLADLSTLEAK
                     K"
BASE COUNT          499 a          753 c          824 g          546 t
ORIGIN      
        1 tagaggagcc aatcggtagg agtgatgtgg cacagacact cctgtctgtg cattccaacg
       61 ctggtagaag ttggtacccc tctgttttca ccgagggaaa ctgccgtcat gaacgacatt
      121 gacggcccgc tggctctcaa acctttctat cgacgcaacc tgccgcacat cgtcaacgca
      181 cggccgctct tcctgacttt caatacgctc aatcgctgga ttctgcctcc cggtgcacga
      241 acgctcgcgc tgcgtcatgc cctgcacgag catcgagaga agatcttcat ggacgttgcg
      301 gtcatcatgc ccgaccacgc gcatctgatc ttcagtctcc tccaagacaa gttcgaagtt
      361 ccctattcgc tggcggatgt gatgaaggga atcaagggcg tttcctcgcg gcggatcaat
      421 cagctgttgg gacgtaccgg gtcgttcgtg cggcaggacg agtccttcga ccatgtcgtc
      481 aggcaaagtg aaaggtcgtt ggccaagtac gagtacgtgt gcaacaaccc tgttcgtgcc
      541 ggcctggtga aacatgcgga tgagtacccg tggctgtgga ggtcatggat tgaaggcaga
      601 ggtagtgaga tggcatgagc agcacagaca agagtgtctg tgccacataa ccatcgcttg
      661 aggggaggag ccttctgcgc ggaggttgcc agtggccaga gtgatgtggc acagacactc
      721 ttgtctgtgc tgctctgtcg gatggactca acccggcgca tcctcaggcc gcccgaacaa
      781 ccatccctgc ccgtacgcga tgccgaggtc tcgcgcggca tcagctgtcg aagcatcctc
      841 gactccctcg atgatgatcg cgcacgagaa ctccgcggcc agtgccacga ggttgcggac
      901 gatctttgtc agcgttgggt cggcttcgaa acgggtgccg aagagttggc tgatcttcag
      961 gtactcgggg cggatgcgct cgattaacgg caggtgcgag taggccacgc cgacgtcatc
     1021 gaaggcgaaa cggacgccga gggtgcgcag atgttcgatg gtttcgaaga gcgcagcagt
     1081 ttgtttgacc tcctgctgtt cggtgatctc gagaacgacg cgcccgaggt cgattccgaa
     1141 gcggtgcgcg ttggattcga gtgcgatcac aacacccaat tcttcgagtg ctgccggatg
     1201 aacgttcatg aagatcagcg aggaaggagg cagcgacgga accgcgcgca tcgtcgcctc
     1261 aatgcaggcc gcctccagcg cggcgacgtc gtgtttgcgt gttgcgtacg cgaagagcag
     1321 gtccggtcgt acgaagggct cgccgccgcg gtaccgcgcc aacgactcga cgccgtgagt
     1381 cgtgccgtca gcaagcgaaa cgatcggttg aaacgcgggg cgcagttgcc cgctgccgat
     1441 cacttcgtcg agcgtcggca ccatcgtcag cgacgaaggc gttgcgtctg aaggaggagt
     1501 cagcagcggc aggagagaat cgatctcgaa cggtttcgac agcacggcat ccgcgccgcg
     1561 ccgttcggct tcggccacga gctcgtcaga tgcgtgaccc gtcatcagga tcacccgtgt
     1621 cgcgggcgac acccgaatga cctcggtgat gaagtcgagt ccttcgaatc ggaacgagcc
     1681 ggaaagcttg acgtcggtga ggatgtcggt gaccggctcg ttttccagaa tgatctgcgc
     1741 cgactccagg tcgcggcaga ccacaatgcg ccggccgtcg cattcgagcg cggcggccag
     1801 cccgatgccg atcaccggct cgtcatcgac gatcaaaagg caggacatgg gggtcaggtc
     1861 taaagtctaa agtgccgaag acgatcgtcg ctaaggcgat caggccaatt cggcacttta
     1921 gactttagac ctgaccctgc gcctcgctac tttgcgttct ttttctttgc agcgggatga
     1981 accgcagccg acttgatccc ggccaggttg gcccggtctt ttgcacgctg cgccgtgcgg
     2041 cgccgttttt cgatcgccgt tcgcgctttg tctccattga tctttgacat gatgcctccg
     2101 gcagccacct tatcaggtcg cgccgtctcg cgagctattt cttcgcctcg agagtggaca
     2161 gatcggcaag cacgtcatcg ctgtgcgtcg ccggtttcac tttgagatag accttgcgga
     2221 tgacgcccga ggggtcgatg aggaacgtgt tgcgcgcggc gtacatcgtg ccgtcgtggt
     2281 ccatcaccga gccgtacgag ctcgaaacgg tgtggctggt gtcggcgaga agcttgaagc
     2341 tgaggccctc tttcgtgcag aagcccttgt gcgaatcgac cggatcgagg ctgacgccga
     2401 cgacgacggc attcttcgcc tcgtacttgg cgatgtcgcg ctggaagttg tgcgcttcga
     2461 gtgtgcatcc cgaggtgaag tctttcgggt agaagtagag gacgacccac tttcccttga
     2521 actgatcgag cgagacgttt gctgcttcct ggctctgcag gctgaacttc ggtgcggcgg
     2581 ttccttccgc gggcggctga ggcgcgtcgg cggcaaatgc gt
//
LOCUS       DATLVW010000891         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_163228, whole genome shotgun sequence.
ACCESSION   DATLVW010000891 DATLVW010000000
VERSION     DATLVW010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163228"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /gene="ftsA"
                     /locus_tag="VNN25_26385"
     CDS             complement(<1..877)
                     /gene="ftsA"
                     /locus_tag="VNN25_26385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXH95130.1"
                     /translation="MPKTEEKYIVALDIGTSKVCVLVGEVTDRGQIEIIGKGTSPMKG
                     TRRGNIINLDQAIEAIKKAVDEAEVMAGLQIESVYVGVAGDHIRSVNSRGVISVTGKH
                     REISREDIDRVIEASKSINIPAELELLHVIPREFVVDGQDGIHDPLGMTAGRLEANVH
                     IVTGARTHNQNVLTGVNKAGIAVQELVLEQLAAAEAVLTQDEREMGVLLMDIGAGTTD
                     YAVFLEGNVVHTNVLPVGAGHFTSDISVVLRTPMEDAERIKKRYGCALASLVTEDDPI
                     EVPTVGGRAPKILSKH"
     gene            complement(896..1747)
                     /locus_tag="VNN25_26390"
     CDS             complement(896..1747)
                     /locus_tag="VNN25_26390"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXH95131.1"
                     /translation="MSAGYDSTSPRFFRPTDVARIRRNQRRIQVQRLAAIAGKAGIVV
                     GLVIGSVLVFQHTRGDNRFAVKSIEVTGVVHTPRAEIDAVTRAYIGENLFKIDIGRLR
                     HDLRGLGWIKRIEAEKKLPDTLRIRIVERTPVALVAGNGRITYVDEEGMAFAELAPSV
                     GDSDLPLITNASGTELQRSVALLQTLRTRDPQIYSRISEVRPIAPSSFALFDRELGAF
                     VYAEDSDLSAKWRDLYAVARAEHFGVHDIEYADLRFAGRIVVKPVHPAALQPVAPKPT
                     ILTAITN"
     gene            complement(1747..>2794)
                     /gene="murC"
                     /locus_tag="VNN25_26395"
     CDS             complement(1747..>2794)
                     /gene="murC"
                     /locus_tag="VNN25_26395"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXH95132.1"
                     /translation="GVSGTHGKTTTSAMIATVLEDAGLDPTIIIGGMLRNFASNAKSG
                     KGDVIVVEADEYDRTFHELRPEIAVITNIEADHLEYYGSFENIVEAFRIFTENVRPVG
                     AIIGCVDDPAVAALLDRRGRSKDPKVIRYGLGPGADLTTRNLTFDERGSSFEVEGVGF
                     FKLFVPGEHNVRNALAVIAVARELGVGHDAIAAGLAKFLGVDRRFQILGDYNGAIVVD
                     DYAHHPTEIRATLSAARSGYPRRRVVALFQPHLYSRTRDFATEFGSSLAGADVALVAP
                     IYAAREQPIDGISSRMIADSAEGVEFIDRSHAEILNELRRRLQPNDVFIAMGAGDVHE
                     IAEALVRGDDGGGV"
BASE COUNT          505 a          890 c          877 g          522 t
ORIGIN      
        1 cgtgcttcga gaggatcttc ggcgcacggc cacccacggt cggcacctcg atcgggtcat
       61 cctcggtgac cagcgacgcg agcgcgcatc cgtagcgctt cttgatccgc tccgcatcct
      121 ccatcggcgt ccgcaacacg acggaaatgt cgctggtgaa atgacccgca ccaaccggca
      181 gcacgttggt gtggacgacg ttcccttcga gaaagacggc gtagtccgtg gtccccgcgc
      241 cgatatccat gagcagcacg cccatctcgc gctcgtcctg cgtcaacacc gcttcggccg
      301 cggcaagctg ctcgagcaca agctcctgca cggcgatgcc cgctttgttc acgccggtca
      361 gaacgttctg gttgtgcgtg cgagcacccg tcacgatgtg gacattcgcc tccagccggc
      421 cggcagtcat gccgagggga tcgtggatgc cgtcctgtcc gtcgacgacg aactcgcgcg
      481 gaattacgtg cagcaactcc agctcggccg gaatgttgat cgacttcgag gcttcgatga
      541 cgcggtcgat gtcttcccgc gagatctcgc gatgctttcc ggtgaccgag atgacgccgc
      601 gcgagttgac cgagcggatg tgatcgccgg ccacgccgac gtagaccgat tcgatctgca
      661 gtccggccat cacctcggcc tcatcgacag ctttcttgat cgcctcgatg gcctgatcga
      721 ggttgatgat gttgccgcgg cgcgttccct tcatcggcga ggtgcctttg ccgatgatct
      781 cgatctgccc gcggtcggta acttccccga ccagcacaca gaccttggaa gtcccgatat
      841 cgagcgcgac gatgtatttc tcttcggtct tcggcattcg ttgtctcctg actggctaat
      901 tcgtgatcgc tgtgagaatc gttggctttg gcgcaaccgg ttgcagcgcg gcgggatgaa
      961 ccggcttcac gacgatgcgt ccggcaaagc gaagatcggc gtattcgatg tcgtgaacgc
     1021 cgaaatgctc ggcgcgcgcg accgcataaa ggtcccgcca ctttgcggaa agatcgctgt
     1081 cctcggcata gacaaacgcg cccagttcgc ggtcgaagag cgcgaacgag ctcggcgcga
     1141 tcgggcggac ctccgagatg cgcgagtaga tctgcggatc gcgcgtgcga agggtctgca
     1201 agagcgcgac gctgcgctgc agctcggtac ccgaggcatt ggtgatgagc ggcagatcgc
     1261 tgtcgccgac agaaggcgcc agctcagcga acgccatccc ctcttcgtcg acgtaggtga
     1321 tgcgcccgtt ccccgcgacg agtgccacag gcgtgcgctc gacgatgcga atgcgcaacg
     1381 tgtcgggaag cttcttttct gcctcgatac gcttgatcca cccaaggccg cgaagatcgt
     1441 gacgcagacg gccgatgtcg atcttgaaga ggttctcgcc gatgtacgcg cgggttacgg
     1501 catcgatctc ggcacgcggc gtatggacga cgcccgttac ctcaatcgac ttgactgcga
     1561 aacggttgtc gccgcgagtg tgctgaaaga caaggaccga accgatgacg agacctacca
     1621 cgatgccggc ctttccggcg atcgcggcaa gacgctgcac ctgaatccgg cgctgattgc
     1681 ggcggatgcg cgcgacgtcc gtggggcgga agaagcgagg cgaggtgctg tcgtaaccgg
     1741 ccgacatcag acccctcctc catcgtcgcc gcgcaccagc gcctcggcga tctcatgaac
     1801 gtcgcccgca cccatagcga tgaacacatc gttcggctgc aaccgccgcc gaagctcgtt
     1861 gaggatctcg gcgtggcttc gatcgatgaa ttccactccc tccgccgagt cggcgatcat
     1921 tcgggaggag ataccgtcga tgggctgttc ccgcgctgcg tagatcggcg cgaccagcgc
     1981 tacatcggca ccggccagcg aggaaccaaa ctcggtcgca aaatcgcggg ttcgcgaata
     2041 gagatgcggc tggaacagcg ccacgacacg acgacgcgga tagccgctgc gcgctgcgct
     2101 cagcgtggcc cggatctctg tcggatggtg cgcatagtcg tcgacgacga tcgctccgtt
     2161 gtagtcgccg aggatctgga agcggcggtc gacgccgagg aacttggcga gaccggcggc
     2221 gatggcatcg tgaccgactc cgagctcacg cgccacggcg atcaccgcca gcgcattgcg
     2281 aacgttgtgt tcgccgggaa cgaagagttt gaagaagccg actccttcga cctcgaacga
     2341 agaaccgcgc tcgtcgaacg tgaggttgcg cgtcgtcaga tcggcgccgg gcccgagtcc
     2401 gtaacggatg actttcgggt ccttcgacct cccgcggcga tcgagaagcg ccgccacggc
     2461 cggatcatcg acacagccga tgatcgcgcc gaccggccgt acgttctccg tgaagatccg
     2521 gaacgcttcg acgatgttct cgaagctgcc gtagtactcg aggtggtccg cctcgatgtt
     2581 cgtgatcacg gcgatctcgg gccgaagctc gtgaaaggtg cggtcgtact cgtccgcttc
     2641 gaccacgatc acgtcgccct tgccgctctt ggcgttcgac gcgaagtttc ggagcatgcc
     2701 gccgatgatg attgtgggat cgagacctgc gtcttcgaga accgtcgcga tcatcgccga
     2761 cgtcgtcgtc ttgccgtgcg taccggagac gccg
//
LOCUS       DATLVW010000892         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_615477, whole genome shotgun sequence.
ACCESSION   DATLVW010000892 DATLVW010000000
VERSION     DATLVW010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615477"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="sdaAA"
                     /locus_tag="VNN25_26400"
     CDS             <1..942
                     /gene="sdaAA"
                     /locus_tag="VNN25_26400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HXH95133.1"
                     /translation="PMIESFAHLEKLAGGRDLADVFLESDEQEHAVPGDQILEALRRR
                     RAVMRDCVERGKGGGTSMGKLVGNEGKLLNDAFLAGKTFMDPLTVKAEIYALSVMGEN
                     SRMGVIVAAPTAGAGGVVPGMLLALEEERHIDPERTVRALVVAGGVGSIIALSANISG
                     AAGGCQNEVGVASAMGAAAVAYMMGGSTRQSIHSAAIALKNTLGLVCDPVGGLVEVPC
                     VKRNALFAVHGMTAAQLALAGVESVIPMDEIVEAMVRIGRALPRGLRETAEGGLATTL
                     TGCTIGDRLKNEANDRRAERAAEREKAAQERKESVSS"
     gene            1068..1196
                     /locus_tag="VNN25_26405"
     CDS             1068..1196
                     /locus_tag="VNN25_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95134.1"
                     /translation="MPADETSALLARRSAVVSTAAIGASRLGGWILGRMTFEVSLP"
     gene            complement(1249..>2592)
                     /locus_tag="VNN25_26410"
     CDS             complement(1249..>2592)
                     /locus_tag="VNN25_26410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXH95135.1"
                     /translation="HAPTVRILPLPDELLSGTQHIETDIEGAGITVVEFYLDGQKVMS
                     KRQPPYALDLDLGSVPQVHRVRVLAMNDKGEMIAGDDAIVNSGNDPFRVRIVSPRIAR
                     KLHGPTRVEMAVNVPEGKQLDKLELFLNDTRVATLYGPPYTQVVDVPESEGIVYLRAV
                     ASLKDVAEQPPIEDVVMFNTPQFSEEVNVHLVELPTTVLRNGHTVNDLQEAAFTVLDD
                     GKPVKVAKFEHVTNLPLSIGLAIDTSGSMQPRMAEAQKAASQFFSNTLRKGDRAFLVS
                     FDSQPRLEQRWSPNLADINAGLAKLRADESTALFDAIVYSLYNFAGVRGQKALVVITD
                     GKDTASKFSFDQAIEYARRAAVPIYGIGIGIKATEVDVRYKFGRFCSETGGNVYYIDR
                     AEDLHRIYADIQNELRSQYILGIYPPEGVKPGSKWHEINVQVSDGKTKTLRGYYP"
BASE COUNT          434 a          795 c          873 g          490 t
ORIGIN      
        1 cccatgatcg aaagctttgc ccacctcgaa aaactcgctg gcggccgcga ccttgcggac
       61 gtctttctgg agagcgacga acaggagcac gccgttcccg gcgatcagat cctcgaagcg
      121 ttgcggcggc ggcgcgcggt catgcgcgat tgcgtcgagc gcggcaaggg cggcgggacg
      181 tcgatgggga agctcgtcgg caacgaaggg aagctgctta acgacgcctt cctcgccggt
      241 aagaccttca tggatccgct cacggtcaaa gcggagatct acgcgctctc cgtgatgggc
      301 gagaactcgc gcatgggcgt gatcgtcgcc gcgccgacgg caggcgcggg tggtgtcgtc
      361 cccggcatgc tgctggcgct ggaggaagag cgccacatcg atccggagcg aacggttcgc
      421 gcgctggtcg tcgctggcgg cgttggctcg atcatcgcgc tctcagcgaa catctccggc
      481 gccgccggcg gttgccagaa cgaggtcggc gtcgcctcgg ccatgggagc agcagccgtc
      541 gcctacatga tgggcggcag cacgagacaa tcgattcact ccgccgcgat cgcgctcaag
      601 aacacgctcg gcctcgtctg cgatccggtc ggcggtctcg tcgaggtgcc atgcgtcaaa
      661 cgcaacgcgc tctttgccgt acacggaatg actgccgcgc aactcgccct ggccggcgtc
      721 gaatccgtca tcccgatgga cgagatcgtc gaagcgatgg tccgaatcgg ccgcgccctc
      781 ccgcgcggcc tgcgcgagac cgccgagggc ggcctggcca ccacgctgac cggctgcacg
      841 atcggtgacc ggctgaagaa cgaagccaac gatcgccgcg cggaacgtgc cgcggagagg
      901 gagaaggccg cgcaggagag gaaggaatcc gttagctctt aggcagccgc cgacgcatga
      961 gacggtgaga ttcgcgcccg gcgcttccgc atgctgaagc cttcacgcca aggacgtcga
     1021 cgaattttcg acctccgccc tgaccgcact cgggcgagga cgcccgagtg cccgcggacg
     1081 agacgtccgc actccttgcg cggaggagtg cggtcgtctc gaccgcggcc atcggggcgt
     1141 cccgcctcgg tggttggatc ctcgggcgca tgacgttcga agtctcgctc ccctgacgga
     1201 ctgagtgtct ggtttgccgt attcctcaac gccgagggag aactgaacct acgggtaata
     1261 ccccctcaac gttttcgtct ttccgtcgct cacctgcacg ttgatctcgt gccattttga
     1321 gccgggcttg acgccttccg gcgggtagat gccgaggatg tattgggagc ggagctcgtt
     1381 ctggatgtcg gcgtagatgc ggtggaggtc ctcggcgcgg tcgatgtagt agacgttgcc
     1441 gccggtctcg ctgcagaagc ggccgaattt gtagcggacg tcgacctcgg tggccttgat
     1501 gccgattccg atgccgtaga tcgggacagc ggcgcggcgc gcgtattcga tggcctgatc
     1561 gaacgagaac ttcgaggccg tgtccttacc gtcagtgatc acgacgagtg ctttttgtcc
     1621 gcgcacgccg gcgaagttgt agagcgagta gacgatggcg tcgaagagcg cggtcgattc
     1681 atccgcgcgc aatttcgcga gcccggcgtt gatgtcggct agattcggcg accagcgttg
     1741 ttcgaggcgt ggctgcgaat cgaacgacac caggaaggcg cggtcgcctt tgcgcagtgt
     1801 gttgctgaaa aactgtgatg cagctttctg cgcttcggcc atgcgcggct gcatcgatcc
     1861 cgaggtgtcg atggccaggc cgatggagag gggaaggttc gtcacgtgct cgaacttcgc
     1921 caccttcacc ggcttgccgt catcgagcac cgtgaacgct gcctcttgca gatcgttcac
     1981 cgtgtggccg ttgcgcagca ccgtcgtcgg cagctcgacc aagtggacgt tgacctcttc
     2041 gctgaactgc ggcgtgttga acatcacgac atcttcgatt ggaggttgct cggcgacgtc
     2101 tttgagcgag gcgacggcgc ggaggtagac gatgccttcg ctttcgggga catcgacgac
     2161 ttgcgtatac ggcggaccgt agagcgtcgc cacgcgggtg tcgttgagga agagctcgag
     2221 cttgtcgagc tgcttgcctt ccggaacgtt gaccgccatc tccacgcggg ttgggccgtg
     2281 cagcttgcgg gcgatgcgcg gcgaaacgat acgcacgcgg aacggatcgt tgccgctgtt
     2341 cacgatcgca tcgtcgccgg cgatcatttc tcccttatcg ttcatcgcga gaacgcgcac
     2401 acgatggacc tgcggcacgc tgccgaggtc gagatcgagc gcgtagggtg gctggcgctt
     2461 gctcatcacc ttctgtccgt cgaggtagaa ctcgacgacc gtgatcccgg cgccttcgat
     2521 gtccgtttcg atgtgctgcg tgccgctgag caattcgtcc ggcagcggaa gaatgcgcac
     2581 cgtcggcgcg tg
//
LOCUS       DATLVW010000893         5466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_407691, whole genome shotgun sequence.
ACCESSION   DATLVW010000893 DATLVW010000000
VERSION     DATLVW010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407691"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VNN25_26415"
     CDS             complement(<1..1255)
                     /locus_tag="VNN25_26415"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXH95136.1"
                     /translation="MDAAHMTAETFSAPRPVSNSVFVEIGRFARLSIVGFSLLLPLAG
                     AAAAAEHVSARQLAALIAVGLAFHVFAYVSNDVFDLPIDRTEPLRAGSPLVRGLIRPS
                     LALVIALVPVPLTAALHLWAGGTPAAAAALLTAMALMLIYNRYGKRVAFPLASDAVQA
                     IGWVALALYGAFATTSAALTEPLGWLAAIVFVYVLLINGLHGGLRDIRNDAAHGARTT
                     AIWFGAGADDDGRLLVPRSLVRYGAALQLVLFFLGVLSVRRWPLAVAMIIAAYVVLFF
                     LGREALRADATKREVIRAGFRHLYLSLGVVLVPFALFGSASVATAIVAVYAVPVVILA
                     LRMLRVRPLLPVALLLAASLGAEPVRVVTKEELIEAMRLQQGYAILATPNGARFNSGL
                     ILHLARAAYAADPNGPPLFIDHRTYF"
     gene            complement(1237..4401)
                     /locus_tag="VNN25_26420"
     CDS             complement(1237..4401)
                     /locus_tag="VNN25_26420"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXH95137.1"
                     /translation="MVRVFISYAREDGEAFARGLEAKLQEYNPWYDRRQLIPGLLWDE
                     KIVEGIDQCDVFLVVLTRGYERGEFALKELQRAIDQRKKVIPLRFHADAGLPIEIQRH
                     QWILFTNGLDDDESSVRLREAIDLLARGGSLTARDLRAGRWDLVQLQTAEWSQEVVRA
                     HFDEKLYVAREASDEQFALFRKSATPALLIVGDSGTGKSSLLGRWTLDLLGDGDAVLL
                     YDCGALAEPDVEEEIARDFGIPREQLLDELEPGAAQAGKKVWFVFDSIGDYRGAESAP
                     VLLRSINKLVERVHAEQVRVIASCNSGMWGRFAFRPGPRWFHSEDDAFLLLDKFTGSE
                     RNEAYRHYREAFHLHSPIEQLDPVVQEQLREPIILRMAAEAYGDVAEPIRRGGLGVRV
                     FHKFLEDRVTTLGAEALVDDLAGAMWQEKKSALSLAELKKYPQIRAQIVAEDGRSAYV
                     PLLDSGVLQQLHDPRSGTVVRFSHTRIASYAVAKSLLGSGSILDTTRSVVADANQFPL
                     AWQTAKTLLLLSRDETAIEALASSHDAEERELASEALVELHADDARAACDLLQKLLNQ
                     PSEEGRRTALKAAYSIGPAVRDFFMRAAIDGGESLRESVRNTLYLIWRRESPAGRRAV
                     ADTIYLIGEHSRGFTYGFLTSMIDDFRITKVKKIRPTLEFALDLIITIYINHCDEDEV
                     IEQTAKLMHTLTVEKLPMPLLLKFERLDLLRKVVLRAFTRIFGSPILDFMMFGNPRSV
                     QEFFRLPAERRASLGRIGDAFDPAADIAAHHDQLAAMLSADLPLFSGSAGLAIAVHAA
                     HDFAATEPLVRQLWNESGPRARLWLLISFSVLIKDTPPEWVALLADLTRQYIEQHRDE
                     FLGDGSRLPGGLDIVALPLGLAYSKAGLSMSLFDDLLRDALAKNDTPLAARIITGLAA
                     VGFYDPLALFGILDPAFAYLGDERIADALVTTLAIVRTLHFDAVDRFLHEKNTPPPLR
                     HRVDTTAGVALVHDYIRVLGYYNNAVHFTLRYPRMREPLSTGALKLLATTPSAPAFAT
                     DYTMTAIRMLRDSEFDLKKWTLPI"
     gene            complement(4639..5061)
                     /locus_tag="VNN25_26425"
     CDS             complement(4639..5061)
                     /locus_tag="VNN25_26425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXH95138.1"
                     /translation="MRTTESRTRVRYKETDQMAIAHHANYVVWFEIGRTDLCREAGFP
                     YREIEERGLILVVTEIVCRYRAPYKYDDEVVIRTSVAEAASRMMRFDYELRDATGERL
                     HATGSSSHVWVDRTSRRPVVADTDVMKGFDPWLGAHSE"
     gene            5110..>5466
                     /locus_tag="VNN25_26430"
     CDS             5110..>5466
                     /locus_tag="VNN25_26430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95139.1"
                     /translation="MWRKRKNDWLNEIANSLLIGGATVLALDLGRRLFRRSQIFCPEP
                     DPVISWNPEDYGIPHGQTEVLRFETGDGEELYGWYCRAKNPIASALYCHGNTGNLSTP
                     AHVMPYLLDAGINILLF"
BASE COUNT          967 a         1768 c         1840 g          891 t
ORIGIN      
        1 cgaagtatgt gcggtgatcg atgaacagcg gcggcccgtt cggatcggcg gcatacgcgg
       61 cgcgggccag gtgcaggatc aggccggagt tgaagcgcgc gccgttggga gtggccagga
      121 tggcgtagcc ctgctgcaag cgcatcgcct cgatcagctc ttctttcgtc accacgcgta
      181 ccggctccgc gcccagcgac gccgccagca gcagcgcgac cgggagcagc gggcgcacgc
      241 gcagcatgcg caaggcgagg atgaccaccg gcaccgcgta caccgccacg atggcggtgg
      301 ccaccgacgc gctgccgaag agcgcgaacg gcacgagcac gacgccgagc gacagataga
      361 gatgccggaa gccggcgcgg atcacctcgc gtttcgtggc gtcggcacgc aaggcttcgc
      421 gcccgagaaa gaagagcacg acgtatgcgg cgatgatcat tgccacggcg agcggccacc
      481 tccggacgct gagcacgccg aggaagaaga gcaccagttg cagcgcggcg ccgtatcgca
      541 cgagcgatcg cggcacgagc agccgcccgt cgtcatccgc accggcgcca aaccagatcg
      601 cggtcgtgcg cgcgccatgg gccgcgtcgt tgcggatgtc gcgcagcccg ccgtggaggc
      661 cgttgatgag gaggacgtag acgaatacga tcgccgcgag ccagcccagc ggctcggtga
      721 gggcggcgct cgtcgtggcg aacgcaccgt acagcgccag cgccacccag ccgatcgcct
      781 gcacggcgtc gctcgccagc gggaaggcga cgcgcttgcc gtagcggttg tagatcagca
      841 tcagggccat ggccgtcagc agcgccgcgg ccgccgccgg cgtaccgccc gcccagaggt
      901 gcagggccgc ggtgagcggt accgggacga gtgcaatgac caacgccaag gagggacgga
      961 tcagtccccg cacgagcggc gagccggcgc gcagcggttc cgtccggtcg atcggcagat
     1021 cgaagacgtc gttcgagacg taggcgaaga cgtgaaacgc cagtcccacc gcgatcagcg
     1081 ccgccagttg ccgcgccgag acgtgctccg ccgccgccgc cgctcccgcc agcggcagca
     1141 gcagcgagaa gccgacaatg gagaggcgcg cgaagcgccc gatctcgacg aagacgctgt
     1201 tcgacacggg gcggggagcg gagaacgttt cggcggtcat atgggcagcg tccatttctt
     1261 caggtcgaac tccgagtctc gcagcatgcg gatggccgtc atcgtgtagt cggtggcgaa
     1321 cgcgggcgcg ctcggtgtcg tggccagcag cttcagcgct ccggtggaga gcggctcgcg
     1381 catgcgtgga tagcgcagcg tgaagtgcac ggcattgttg taatagccga ggacacggat
     1441 gtagtcgtgt acgagtgcca ctccggccgt ggtgtcgacg cggtggcgga gcggcggggg
     1501 agtgtttttc tcgtgaagga aacggtcgac cgcgtcgaag tgcagcgtac gcacgatggc
     1561 cagcgtcgtt acgagcgcgt cggcgatccg ctcgtcgccc aggtaggcga aagcgggatc
     1621 gagaatgccg aacagcgcca gaggatcgta gaagccgacc gcggccagac cggtgatgat
     1681 gcgcgcggcc agcggcgtgt cgttcttcgc cagtgcgtcc cgcagcaggt catcgaacag
     1741 cgacatcgac aatcctgcct tgctgtacgc gaggccgagg ggcagcgcga cgatgtccag
     1801 ccctccgggg agccggctgc cgtcgccgag aaactcgtcg cgatgctgct cgatgtactg
     1861 gcgcgtcagg tccgcaagca gcgcgaccca ttccggcggc gtgtctttta tgaggactga
     1921 gaaggagatc aggagccaga gacgcgctct tggaccactt tcgttccaga gctgccgcac
     1981 cagtggctcg gtcgcggcga aatcgtgcgc cgcgtgaacg gcgatagcca ggcccgcgga
     2041 gccgctgaag agcggcaagt ctgccgagag catcgccgcg agctggtcgt ggtgcgccgc
     2101 aatgtccgcg gccggatcga acgcgtcccc gatccggccg agggaggcgc gccgctcggc
     2161 cggcagccgg aagaactctt gcaccgaacg gggatttccg aacatcatga aatccaggat
     2221 cgggctgccg aagatccgcg tgaacgcacg aaggacgacc tttcggagca gatcgagccg
     2281 ctcgaatttc aacagcagcg gcatgggcag cttctcgacg gtcagcgtgt gcatcagctt
     2341 ggccgtctgc tcgatcacct cgtcctcgtc gcaatggttg atgtaaatgg tgatgatgag
     2401 gtcgagcgcg aactccaggg tgggcctgat cttcttgact ttcgtgatcc ggaagtcgtc
     2461 gatcatgctc gtaaggaagc cgtaggtgaa ccccctcgag tgctcgccga tcagatagat
     2521 cgtgtcggcg accgcgcggc ggccggcggg cgactccctg cgccagatca ggtagagcgt
     2581 gttccggacc gattcgcgca acgattcacc gccgtcgatg gcggcgcgca tgaagaagtc
     2641 acgcaccgcc ggaccgatgc tgtacgccgc cttcagcgcg gtgcgccgcc cttcctccga
     2701 cggctggttg aggagcttct gcaggaggtc gcacgcggcc cgcgcatcgt cagcatgcag
     2761 ctcgacgagc gcctccgagg ccagttcgcg ctcctccgcg tcgtgggacg acgccagcgc
     2821 ctcgatggcc gtttcgtcgc ggctcaggag cagcagcgtc ttcgccgtct gccacgccag
     2881 cgggaactgg ttcgcatcgg cgacgacgct gcgcgtcgta tcgaggatgc tcccgctgcc
     2941 caggagcgac ttcgccaccg cgtacgacgc gatgcgggtg tgggagaagc gcaccaccgt
     3001 cccggagcgg ggatcgtgga gctgctgcag cacgccgctg tcgagtaggg gcacgtacgc
     3061 cgaccgcccg tcttcggcga cgatctgcgc ccggatctgc ggatactttt tcagctcggc
     3121 cagtgagagg gcgctcttct tctcctgcca catcgcgccg gcgagatcgt cgaccagcgc
     3181 ttccgcgccg agcgtggtga cgcggtcttc gaggaacttg tggaagacgc ggacgccgag
     3241 gccgcctcgg cggatcggct cggccacgtc accatacgcc tccgcggcca tgcgcaggat
     3301 gatcggctcg cgcaactgct cctgcacgac cgggtcgagc tgttcgatcg gtgagtgaag
     3361 gtggaacgcc tcccgatagt ggcggtacgc ctcgttgcgc tcgctcccgg tgaacttgtc
     3421 cagcagcagg aacgcgtcgt cttcggaatg gaaccagcgc ggcccggggc ggaacgcgaa
     3481 gcgtccccac atgccgctgt tgcagctggc gatgacgcgg acctgctccg catgcacgcg
     3541 ctcgacgagt ttgttgatgc tgcgcaggag caccggcgcc gactccgcgc cgcgatagtc
     3601 tccgatcgaa tcgaagacga accagacctt cttcccggcc tgcgccgccc cgggctcgag
     3661 ctcgtccagc aactgctcgc gcggaatgcc gaagtcgcgg gcgatctctt cctccacgtc
     3721 gggctcggcc agcgcaccgc agtcgtatag cagtacggcg tctccgtcgc cgaggaggtc
     3781 gagtgtccag cggccgagga ggctgctctt gccggtgccg gaatcgccga cgatcagcaa
     3841 cgccggagta gcgctcttcc gaaacagcgc gaactgctcg tccgacgcct cgcgggcgac
     3901 gtagagcttc tcgtcgaaat gcgcgcggac cacttcctgc gaccattcgg ctgtctgcaa
     3961 ctgcacgagg tcccagcggc cggcgcgcag atcgcgcgcg gtgaggctgc cgccgcgggc
     4021 gaggagatcg atggcctcgc gcagccggac cgagctctcg tcgtcatcga ggccgttggt
     4081 gaacaggatc cactggtggc gctggatctc gatggggagg ccagcgtcgg cgtggaaacg
     4141 gagcgggatg accttcttcc gctgatcgat ggcccgctgc agctccttga gcgcgaactc
     4201 gccgcgctcg tagccgcgcg tgagaacgac gaggaagacg tcgcactggt cgatgccttc
     4261 tacgatcttc tcgtcccata gcaaaccggg aatcaactgc cgccggtcgt accacgggtt
     4321 gtattcctgc agcttcgcct cgaggcctcg cgcgaatgcc tcaccgtctt cccgcgcata
     4381 cgagatgaag acccggacca ttgtctgttg gaatgcttga cggaagaata tcaccacccc
     4441 ggcaaacgga acgtgccgat gcccacgtac tgcagagaga ggctcgatgc caggacgaaa
     4501 tcgtcaccgc ggccgagtcg cttctgcagc ggcgactcgc cctgaaccga attgtcgacc
     4561 gacagtgcgg aacacggtcg tctcgaacgc aacctacgcg aggctctcgg gcatcggcac
     4621 gggcccgcca acgcagactc actcgctatg tgcccccagc cacgggtcga atcccttcat
     4681 cacatccgtg tccgccacca ctgggcggcg tgatgtgcgg tccacccaga cgtgcgatga
     4741 gcttccggtg gcgtgcaggc gctcgccggt ggcgtcgcgc agctcgtagt cgaagcgcat
     4801 catccggctt gcggcttcgg cgaccgaggt gcggatcacg acttcgtcgt cgtacttgta
     4861 gggggcacgg tagcggcaga cgatttcggt gacgacgagg atcaatccac gctcctcgat
     4921 ctcgcgatac gggaatccag cctcgcggca aaggtccgtc cgtccgattt cgaaccaaac
     4981 gacgtaattc gcgtgatgcg cgatagccat ctggtcggtt tccttgtacc gtacacgggt
     5041 gcggctttcg gtcgtgcgca tgtcgcgcgc gattctaacc cggcagatct tttgctcttc
     5101 gaagggatcg tgtggcgcaa acgaaagaac gactggctta acgagatcgc aaactcgttg
     5161 ttgatcggcg gtgcgacggt cctggccctc gacctcggac gccgcctctt ccgacgttcc
     5221 cagattttct gccccgagcc tgatccggtg atcagttgga atccggagga ttacgggatt
     5281 ccacatggac agaccgaggt cctgcgcttc gaaacgggcg acggggagga gctgtacggc
     5341 tggtattgcc gggcgaagaa tccgatcgcc tccgcgcttt actgccacgg aaacaccggc
     5401 aacctgtcca ccccagcgca cgtcatgccc tatttgctgg acgccggaat caacatcctt
     5461 cttttt
//
LOCUS       DATLVW010000894         8142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_534118, whole genome shotgun sequence.
ACCESSION   DATLVW010000894 DATLVW010000000
VERSION     DATLVW010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534118"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1651)
                     /locus_tag="VNN25_26435"
     CDS             complement(17..1651)
                     /locus_tag="VNN25_26435"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXH95140.1"
                     /translation="MANEDEAGGVNRRHFLELAALGSAVALAKPLSAVAQAASAPVSV
                     KPELEEITISELQDGMKSGRWTARSIAEGYLARIDALDKRGPAINAIIEINPDALSIA
                     DAMDAERKAGHVRGPLHGVPVVIKDNIDTADKMKTSAGSLALAENVAARDAFVVSRLR
                     EAGAIILAKTNLSEWANFRSTHSTSGWSGRGGLTLNPYVLNRNACGSSSGTGSAIAAN
                     LAAIGVGTETDGSVVCPSSASGLVGIKPTLGLISRAGIIPIAHSQDTSGPMARTVRDA
                     AILLGILTGADERDPITVSSRTKGHSDYTRFLDAEGLKGARIGVARQYFGFNDATDKL
                     MKDAIALIQSLGATIVDPANIATNGKFDATENDVLQFEFKDGINQYLANAPSSVKSRT
                     LADLIKFNEEHRAEEMPYFGQEIFEQSEKRGPLTEKKYLDALAKNHRMSRTEGIDATI
                     AKHKLDAIIAPTGGPVWTTDLVNGDHFTGGYSTASAVAGYPHITVPAGLAFGLPVGLS
                     FFGGAWSEPSLIKYAYAFEQAAKARQRPKFLSATSA"
     gene            1707..3185
                     /locus_tag="VNN25_26440"
     CDS             1707..3185
                     /locus_tag="VNN25_26440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95141.1"
                     /translation="MKKLPLLLMLLALLPARMFAFDNTYYRSLAAMPLAVDACSRTGL
                     PDDQLGRFALQLNDANVPPPLFVETVRYAPIVLQQPDFFPFVDNEISQGVTGTALVTV
                     INQRLPRYGVTVRRIETTPAWVLSDNYIPRYDSYSSRRAVYSTRYTHPRYDRRSIQFL
                     IEMPLAVATVSELDGIREDDLGRFCGALNQGYLPPEEFVDTVRYAPAVFVERPDFVPF
                     IEAQIGNGVIGIQLYDTIYPRLPSYSTRSIAFTPDYVLNDQYYIPQPVRTVFTQAQLN
                     GGYPQQAYGQTYVPQPAIAQPVYTPQPVYTQQPVVQPSYAQQRGNRGWQQPRAVAQPS
                     FVPQPSFVPQPGMAQGQYVAPQVAHIPPGQAKKAERHAEMEVRQEHGNGHGHGRGQVF
                     QQPGQVFQQPIAAPPPMASAVPPVVRQEHGNGHGRGRAVQQPIVAPPPMASAVPAPAP
                     APVFVPPPPPQNNGQEHGRGPGGQGPPGQQKEKDKGKGHGKD"
     gene            3190..3510
                     /locus_tag="VNN25_26445"
     CDS             3190..3510
                     /locus_tag="VNN25_26445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95142.1"
                     /translation="MRTTFITLALSAALIAGGCSKANQTATTSSSSQPTAGSSVAATA
                     APAMTPEQLGQLGALINKQPANADKLLADQGLTQETFESAIRKVSSDPAASKRYAAAY
                     KSAS"
     gene            complement(3512..4570)
                     /locus_tag="VNN25_26450"
     CDS             complement(3512..4570)
                     /locus_tag="VNN25_26450"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXH95143.1"
                     /translation="MKRFAIFSLLAFAVSASAATYEARTAHYAVVIHALPAAGDDVVY
                     DVTVSELPGENTLASVPMRAKAGAAVDKQVDVDGQQVSIHLAPAGNVMAYKIEIRKGD
                     VIDSIQSAGATPSARAMYAPGDPPLRVGGDVKAPVVIHRVEPIYTVLARKSRISGIVI
                     LEAIIDHTGVVKDVQILKPLPFGLDQAAIDAVKQWTFRPGMLNGQPVDVIFNLTILFR
                     APEPSTSAVDSTQSSGATQPVLSSNAAGGPPFHVGGDVTPPIVLDHVEPVYPVEARRA
                     RISGIVIVEAVIDRAGVVKDVRVIKPLPFGLSEAAIKAVKQWKFRPGTLYGKPVDVLF
                     NLTVNFHMSQPPPQPPPD"
     gene            complement(4830..5312)
                     /locus_tag="VNN25_26455"
     CDS             complement(4830..5312)
                     /locus_tag="VNN25_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95144.1"
                     /translation="MNRLLRSLVVVSIVAAPAVFAAEVKETVHVVITGGANAGTYDGT
                     TDRGGCSAGMTGKGSWGNQFSLPKENNPKVLNSVQLIVPDAKGAASGTHSFFLNVAFG
                     RITARVAEYKVDTEHKSGSGTVTVADHGSTAVVTFDATTAAGVKLKGTIDCKSVLRAP
                     "
     gene            complement(5434..5754)
                     /locus_tag="VNN25_26460"
     CDS             complement(5434..5754)
                     /locus_tag="VNN25_26460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HXH95145.1"
                     /translation="MNDPRRGEVWLVDLGLAAKVRPCLVLSVPAADTDRALVALVPHT
                     TSLRGTRFECIVPVGFLKAGAFDVQGIVTVPHAKLLRRLGGLRPEQLAAVEAAVGRWL
                     GLRP"
     gene            complement(5751..5939)
                     /locus_tag="VNN25_26465"
     CDS             complement(5751..5939)
                     /locus_tag="VNN25_26465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95146.1"
                     /translation="MTAAAQHIVENFESLSDAEKREVLANLLRISRDIDYPEATDEEL
                     VGAADTVFLSYDRMEQGE"
     gene            complement(6061..6594)
                     /locus_tag="VNN25_26470"
     CDS             complement(6061..6594)
                     /locus_tag="VNN25_26470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95147.1"
                     /translation="MTTKDTVQTQVPSSDLAATDAAIQQAVAQLQGIYQILPDFVPAP
                     ADGVKGMRSARSFTDAFLEASAVAAENDTTMQASTGLDPVRTRLVITRNLRFEPLAAA
                     AEALARDIRYNMFRERWSVVQQALQVFSLAKGYTRNGRADALVAHVRTMQSALGRTGT
                     RRKVKKPALKLVPQQPQ"
     gene            complement(7471..8031)
                     /locus_tag="VNN25_26475"
     CDS             complement(7471..8031)
                     /locus_tag="VNN25_26475"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HXH95148.1"
                     /translation="MIHFEGTLTPELYRRALGVTGRSGRWVAWVLILAGVVNLFFAHL
                     DRPVTWGMPLLLALFGVMLLIMPRYAVTKAFATDRLLSEPMTGDADEQGVRLESAHGR
                     ADLPWSLMHKVVVTANLVTIYQSASIVRILPRECFADEESWQSLRRLASAVRPAAGPS
                     RPLLVLLLWAAIVIAVVLLWTLFNRT"
BASE COUNT         1595 a         2663 c         2423 g         1461 t
ORIGIN      
        1 ggtttttatc cgcggattac gcagatgtag cagatagaaa ctttggccgc tgacgcgctt
       61 tcgccgcttg ttcgaacgcg tacgcgtact tgatcagcga cggctcgctc cacgcgcctc
      121 cgaagaatga caggccgacc ggtaagccga aggccagtcc tgcgggaacc gttatatgcg
      181 gatatcccgc gacggcagac gctgtcgaat agccgcccgt gaagtggtcg ccgttcacga
      241 gatccgtcgt ccacaccggt ccgccggtcg gcgcgatgat cgcgtcgagc ttgtgtttcg
      301 ctatcgtcgc gtcgatgccc tcggtgcgcg acatccggtg attcttcgcc agcgcatcga
      361 ggtacttctt ctcggtcagc ggcccgcgct tttccgactg ctcgaagatc tcctgtccaa
      421 agtacggcat ctcctcggcg cgatgctctt cgttgaactt gatgagatcc gcgagcgtgc
      481 gggacttcac ggatgacggt gcgttcgcca ggtattggtt gatgccgtct ttgaactcga
      541 actgcagcac gtcgttctcg gtcgcgtcga acttcccgtt cgtggcgatg ttggcgggat
      601 cgacgatcgt ggcgccgagg ctttggatga gggcgatggc gtccttcatc agtttgtcgg
      661 tggcgtcgtt gaatccgaag tactgacgcg ccacgccgat gcgcgcccct ttcagtccct
      721 cggcatcgag aaagcgcgtg taatccgaat gccctttcgt ccggctcgaa accgtgatcg
      781 gatcgcgctc gtcggctccc gtcaggatgc cgaggaggat ggcggcgtcg cggacggtac
      841 gggccatcgg accggacgtg tcctgactgt gcgcgatcgg gatgattccg gcgcggctga
      901 tgagcccgag ggtcggtttg atgccgacga gccccgaggc ggaggagggg cagaccacgg
      961 aaccatcggt ttctgtgccg acgccgatcg ccgccagatt cgctgcgatc gccgatcccg
     1021 tccccgagct cgagccgcag gcattgcgat tgagcacgta cggattcagc gtcagtccgc
     1081 cgcggccgct ccatccgcta gtcgaatgcg tcgatcggaa attcgcccac tcgctgagat
     1141 tcgtttttgc caggatgatc gcgccggctt cgcggagtcg tgagaccacg aaggcatcgc
     1201 gcgcggccac gttctctgcg agtgccaacg aaccggccga cgtcttcatc ttgtccgccg
     1261 tgtcgatgtt gtctttgatc accaccggga cgccgtgcag cggcccgcgc acatgtcccg
     1321 ctttccgctc ggcatccatc gcgtccgcga tcgacagcgc atcaggattg atctcgatga
     1381 tggcgttgat cgccggcccg cgcttgtcga gcgcgtcgat cctcgccaga tatccctcgg
     1441 cgatcgaacg cgccgtccag cgtccgcttt tcatcccatc ctgtagctcg ctgatggtga
     1501 tttcttcgag ctcaggcttt acggaaaccg gcgctgaggc tgcttgtgca accgcactca
     1561 gcggcttcgc aagcgctact gctgagccca atgcggcgag ttccaggaag tgtctgcggt
     1621 tcactccgcc agcttcgtct tcatttgcca tgtaatcaat gataaggcct cgcacttgca
     1681 gtggtggaac agcggatggt gacctcatga agaaactccc actgctcttg atgcttctcg
     1741 cgctgttgcc cgcgcgcatg tttgcgttcg acaacaccta ctaccggtcg cttgccgcga
     1801 tgcctcttgc cgtcgatgcc tgctcgcgca ctggattgcc cgacgaccag ctcggacggt
     1861 ttgctttaca gctcaatgat gccaacgtac cgccgccgct gttcgtcgaa acggttcgct
     1921 acgcaccgat cgtgctgcag caacctgact tcttcccgtt cgtcgataac gagatctcgc
     1981 aaggcgttac cggaacggcg cttgtgacgg tgatcaacca gcggctgccg cgctacggcg
     2041 tgacggttcg ccgcatcgag acgacgccgg cctgggtgct gagcgacaac tacatccctc
     2101 gctacgactc ctactccagc cgccgcgcgg tgtacagcac gcgctacacg catccgcgct
     2161 acgaccggcg atcgatccag ttcctgatcg agatgccgct ggccgtggcc acggtctccg
     2221 agctcgatgg catccgcgag gacgacctcg ggcgattctg cggcgcactg aatcagggct
     2281 atctgccgcc cgaggagttc gtcgacaccg tgcggtacgc gcctgcggta ttcgtcgagc
     2341 gccccgactt cgttccgttc atcgaggctc agatcggaaa cggggtgatc ggcatccagc
     2401 tctacgacac gatctatccg cgccttccct cgtactcgac acggtcgatc gcgttcacgc
     2461 ccgactacgt gctcaacgat cagtactaca tcccgcagcc ggtgaggacc gtcttcacac
     2521 aggcacagct caacggcggc tacccgcagc aggcgtacgg gcagacctac gttccgcaac
     2581 ctgccatcgc acaaccggtc tacacgccac aacctgttta cacgcagcag ccggtcgtgc
     2641 agccgtccta cgcgcagcaa cgcggaaacc gcggatggca gcagccgcgc gcggtcgcgc
     2701 aaccgtcgtt cgtgccgcag ccctcattcg tgccgcagcc ggggatggcc cagggtcagt
     2761 acgttgcacc gcaagtcgcg cacatcccgc cgggacaggc gaagaaggct gagcgacatg
     2821 cagagatgga agtcaggcag gagcatggta atggtcacgg acacggacgc ggccaggtct
     2881 tccagcagcc aggtcaggtc ttccagcaac cgatcgccgc accgccgccg atggcctcag
     2941 ccgttccgcc agtagtcagg caggaacatg gcaacggtca cggacgcggc cgcgccgtcc
     3001 agcagccaat cgtcgcgcct ccgccgatgg cctcagcggt tccagcgcca gcacccgcac
     3061 cggttttcgt tcctccaccg ccaccgcaga acaatggcca ggagcatgga cgcggtcccg
     3121 gtggccaagg ccctcccgga cagcagaaag aaaaagataa aggcaaaggt catggaaagg
     3181 attagcgcaa tgagaacgac attcatcaca ctcgcactct ccgcggcgct catcgccggc
     3241 ggctgcagca aagcgaatca gacggcaacc acctcgtcat cgtcgcaacc gacagcgggc
     3301 agcagcgtgg cagcgacggc ggcgccagcg atgacaccgg agcagctcgg ccagctcggc
     3361 gcgctgatca acaagcagcc ggccaacgcc gacaagctcc tcgccgatca gggcctgacg
     3421 caggaaacgt tcgagagcgc gatccgcaaa gtcagctccg atccggcagc atcgaagcgc
     3481 tacgcggcgg cgtacaagag cgcgagctga ttcaatccgg cggtggctgg ggtggcggtt
     3541 gcgacatgtg gaagttgacc gtgagattga acaacacgtc gaccggtttt ccatagagag
     3601 tgcccggccg gaacttccac tgcttcaccg ctttgatcgc cgcctcggat aacccgaagg
     3661 gcagcggctt gatcacgcgc acgtccttga ccacgccagc acggtcgatc acagcctcca
     3721 cgatgacgat gccggagatc cgtgcccttc gcgcctcaac cgggtaaacc ggctccacgt
     3781 gatcgagaac gatcggaggc gtgacgtcgc cgccaacatg gaacggcgga ccgcctgccg
     3841 cattcgacga gagcaccggc tgcgtcgctc cggagctctg agtcgaatcg accgccgagg
     3901 ttgacggctc cggggcccgg aagaggatcg tgagattgaa gatcacgtcg accggctgtc
     3961 cattcagcat gccgggccgg aacgtccact gcttcaccgc gtcgatcgca gcctgatcca
     4021 gtccgaatgg caacggtttg aggatctgaa catccttgac gacaccggtg tggtcgatga
     4081 tggcctcgag gatgacgatg ccgctgatcc gcgattttct cgccaggacg gtgtagatcg
     4141 gctccacgcg gtggatgacg accggtgcct tgacgtcgcc gccaacgcgg agcggcggat
     4201 caccgggcgc gtacatcgct cgagcagacg gcgttgcccc cgcactctga atcgagtcga
     4261 tcacatcgcc ctttctgatc tcgatcttgt acgccatcac attgcctgcc ggcgccaggt
     4321 ggatggacac ttgctgcccg tcaacgtcca cctgtttgtc caccgcggcg ccggccttcg
     4381 cccgcatcgg cacggacgcg agcgtatttt cgccgggcaa ctcgctgacg gtcacatcgt
     4441 agacgacgtc gtcaccggcg gccgggaggg cgtggatgac gaccgcgtaa tgcgccgtgc
     4501 gggcttcgta agtcgccgcg ctcgcagaaa ccgcaaaggc aaggagcgaa aagatcgcga
     4561 atcgcttcat ggcgcatctc ctcgagaccg tcaggcagtg atgatgcggt cccatcttac
     4621 ggcgggttaa ctcaggaaga aaggcaaggg ctgttcttac cgcccggttg gcggcgagac
     4681 gccgccaagc cagccggcgg gacgccagcg ttccgccggc gacttgcgcg gccagcacaa
     4741 attaacgaac gcccgcgcca cttgcggatg gaacgctggc gtcctcgccg gctggcgtgg
     4801 cggcgtctcg ccgccacatc gcaaacaacc tacggcgccc gcaacacgct cttgcagtcg
     4861 atcgtccctt tcagcttcac gccggcagcg gtcgtcgcat caaacgtgac caccgccgtc
     4921 gatccgtgat cggcgaccgt caccgtcccg ctgccgctct tgtgctccgt gtccaccttg
     4981 tactcggcca cccgcgcggt gatccgtccg aacgcaacgt tgagaaagaa gctgtgcgtc
     5041 cccgacgccg cccccttcgc atccggcacg atcagctgca cactgttcag caccttcgga
     5101 ttgttctcct tcggcaacga gaactgattc ccccacgacc ccttgcccgt catccccgcc
     5161 gaacacccac cacgatcggt cgtcccgtca tacgtcccgg cattcgcccc acccgtgatg
     5221 acgacatgca ccgtctcctt aacctcggca gcaaaaacag caggagcagc aacgatgctg
     5281 acaaccacca gactacgaag caaacgattc aaatgaagcc tcctgttttt ctgcggcttc
     5341 gccgcgttgt cggttgtcgg ttgacggtag accacggcgc ggccacgttt caaattttgg
     5401 tgggtattgt aaacggaggg gacgggcgtt cgtttacggt ctgaggccaa gccaacgccc
     5461 gactgctgcc tcaaccgcag cgagctgctc cggccgaagg cccccaagcc ggcgcaggag
     5521 tttcgcgtgg ggaaccgtca cgatgccttg cacatcgaac gctcccgcct tcaagaagcc
     5581 aacggggaca atgcattcga aacgggttcc gcgaagtgat gtcgtatgcg gaaccagtgc
     5641 gaccagtgcc cgatcggtgt ccgccgccgg aacgcttagg accaggcacg gccggacctt
     5701 tgccgccaga ccaagatcca cgagccagac ctccccgcgt cgcggatcgt tcactcgccc
     5761 tgttccatcc gatcgtagct gaggaacacg gtgtccgccg cgcccaccaa ctcctcgtca
     5821 gttgcctcgg ggtagtcgat atcccgcgag attctgagaa ggttcgcaag gacttcgcgc
     5881 ttttcggcgt cggatagcga ctcgaaattc tccacgatgt gctgcgcagc ggcggtcatg
     5941 gcaagagtat gcaagcgctc gcaccagagt gcaaatttca gaggatgacg ggcaggaacc
     6001 caaaaaaaac gacccgcgcg acgtctcgcg cgggccgttc tcacatctac tgctactgcg
     6061 ttactgcggt tgctgcggaa cgagtttgag tgccggtttc ttcaccttgc ggcgagtacc
     6121 ggtacgcccc agtgccgact gcatcgtcct gacatgagcg acgagtgcgt ctgcacggcc
     6181 gttacgggtg tagcctttag cgaggctgaa cacctgcaac gcctgctgca cgacgctcca
     6241 gcgctcacgg aacatgttgt agcgaatgtc ccgagcaagc gcttcggcag cggcagcaag
     6301 aggctcgaac cgcagattac gggtgatgac cagccgcgtt ctgaccggat cgagtcccgt
     6361 cgacgcctgc atcgtcgtgt cattttcggc agcgacggcg ctggcttcga gaaacgcgtc
     6421 cgtgaacgag cgggccgaac gcatcccctt gacaccatca gccggtgccg gaacgaaatc
     6481 cggcaggatc tgatagatgc cttgaagctg cgcaaccgcc tgctggatgg cggcatcggt
     6541 tgccgccagg tccgaagacg gaacctgcgt ttgaacggta tctttagtag tcatagcctt
     6601 cccctccttt ctgccagggt tgtcctgcgc gcactgctcg gatcgcctga cactaattga
     6661 gacgtgtcag cttttggaag gttacggaat ttggaaagaa agtttgggaa gagagaacgg
     6721 gttcacgcga agccgcggag agcgcgggtt cctcctccgc ccctccgcgc ctccgcgtga
     6781 ccatctcccc tgaccttttc gccccgacca ccccgaatga tccaccaccc gaccgccgtg
     6841 cccccgccgg cgaagccggc gcaagaacgt aacccccgtc ttcagacggg ggacacgcga
     6901 ttccccccgt ttggcgagcc cgcttcagcg ggcgaaagag gggccgtcca cgcgattctc
     6961 tgtcgcccac tgaagcgggc taccaaccag ggggggcgtc cccctctttt cttcgccgga
     7021 tcgcctgacc tgatgaatac gggcgagctg aagaaggggt cacggaaaaa gaggcagggc
     7081 agcgactgtc acaaaaggtg gacaattaca ccaccaaaaa tggaagttag acaatatgag
     7141 aatccgttaa cggtaaacag gaatccatta acggtaaatg ggaatccgtt aacggtaaac
     7201 gggaatccat gaacggtaaa ccggaatccg ttaacggtaa accggaatcc atgaacggta
     7261 aaccggaatc cgtgaatggt aaacgggagt ccgtgaatgg gaaaccgaat cacacgaacg
     7321 tgaaatcaga tcacgtgggc gggaagccgg gacccgtgaa cgggaagtga gagcgggttc
     7381 acgcggagcc gcggaggccg cggggcttct ttctcggccc ctccgcgcct ccgcgtgacg
     7441 attgcccctc ggccccggtg ccccgctccg tcatgtccgg ttgaagagag tccacaggag
     7501 aaccaccgca atcacgatcg cagcccacag cagcagcacg agaagcggcc gcgaaggtcc
     7561 ggccgctggt cgaactgcgc tcgcgagccg gcggaggctc tgccacgact cttcgtcagc
     7621 gaagcactcc cgaggaagaa tgcggacgat gctggccgac tggtagatcg taaccagatt
     7681 cgcagtcacc acgactttgt gcatcagcga ccacggcaaa tcggcccgtc catgcgctga
     7741 ttccagacga acgccctgct catcggcgtc gccggtcatc ggttccgaga gcaaccggtc
     7801 ggtagcgaac gccttcgtca cggcatacct cggcatgatc aacagcatca cgccgaacaa
     7861 cgccagaagc agcggcatcc cccatgtgac aggccggtcg aggtgggcaa agaaaagatt
     7921 gacgactccc gccaggatca atacccaggc gacccaccgg cccgaccggc cggtaacacc
     7981 gagcgcgcga cgatagagct ccggcgtcag cgtcccctcg aaatggatca ttcgcccatt
     8041 ctgaacgaga ttcctcaacc acagagacac cgaggacgca gagagagctg accgcccgtc
     8101 ccgccggcga agccggcgca agatcgtagc ccccgtcttc ag
//
LOCUS       DATLVW010000895        10818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_327374, whole genome shotgun sequence.
ACCESSION   DATLVW010000895 DATLVW010000000
VERSION     DATLVW010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327374"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VNN25_26480"
     CDS             complement(<1..836)
                     /locus_tag="VNN25_26480"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH95149.1"
                     /translation="MDPVSILDGVISNLLFAKALGIDKKLSSIRSVARAISSASAEVP
                     FSDVHRILTVWVDCVDLSEISPGDRAGTQRLIAKYCEVASIAGVSTDELTAASVIERF
                     GVHLDRVRRLDVAEHDARESVRHAGLSEQIAQSAAESRNGHDAILKEIQKLTASVGGS
                     VSKLSPSAKNLASVEALVDAGLFVTARDRLKAIPCEVELDHADAVSYQTFSGICAYHL
                     GDVHGAVQSFQRASDLKPESSKTASNLAAAYLAQGDYDKAIGEARRARSLSPNLAPIN
                     LIA"
     gene            complement(900..3734)
                     /locus_tag="VNN25_26485"
     CDS             complement(900..3734)
                     /locus_tag="VNN25_26485"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXH95150.1"
                     /translation="MTSIEVPSSPLRPRSNSKPARKKETKGGGIEVERFFTKAGVDVY
                     DTCEWETRAAAISNESGGIVFEQKDVEMPKFWSQMATNVVVSKYFRGHLGSPDRETSV
                     RQLIGRVVRTITAWGRQGGYFNTEADGDTFRDELTHILLYQMAAFNSPVWFNVGIEPH
                     PQCSACFINSVNDTMDSILGLAKTEGMLFKYGSGTGSNLSSIRSSRELLAGGGTASGP
                     VSFMKGYDSFAGVIKSGGKTRRAAKMVILNADHPDILDFIESKEKEEKKAWALIDAGY
                     DGAFNAPGGAYDSVQFQNANHSVRVTDEFMKAYEKGEDWNTYTVTEPKTVMGTYKARD
                     LMRKMAESAHLCGDPGMQFDTTVNDWHPCPNTARINASNPCSEYMFLDDSACNLASLN
                     LMRFYNENTGFDVEAYRAALRIVITAQEITVDFASYPTKAIEKNSHAFRPLGIGYANL
                     GALLMARGIAYDSEDGRDYAAAITSILSGESYAQSARIAAKVGPFEGYAINQEPFLRV
                     IDKHRRAAYRINNRPLPADLAEAATQVWDEAYALGQEHGYRNAQISVLAPTGTIAFMM
                     DCDTTGIEPDIALVKYKKLVGGGMLKIVNQTVPTALKRLGYDPKQIGEIIQYIDENDT
                     IEGAPHLLDQHLTVFDCAFKPKNGTRSIHYIGHLKMMGAVQPFISGAISKTINMPANA
                     TIDEIELAYVEAWKLGLKAVAVYRDGCKRSQPLSTGKDKDKLTDLGVAPTAVRRKLPD
                     ERESITHKFSISGHEGYITVGKYEDGTPGEIFITMAKEGSTISGLMDSFATMTSLALQ
                     HGVPLNFLVDKFTHTRFEPSGFTKNPEIPMTKSIMDYIFKWLATKFLDRESQTQAGVI
                     VREEAPAPAPKPLPNVAPLSKDDATFVSSLGTSPIIAAPKDADGPNARNTATFRYQQD
                     APSCSDCGSIMVRNGACYKCLNCGSTSGCS"
     gene            complement(4537..5583)
                     /locus_tag="VNN25_26490"
     CDS             complement(4537..5583)
                     /locus_tag="VNN25_26490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95151.1"
                     /translation="MSWLPGPSVEEERTEALRRLHVADVAATPWRHNGIMLRIVFSVL
                     AIVALAALFGFFAILSLPKEWLTGIAAIGAAEWLIRRHHFFSTGLESTLWIAGLFCLI
                     FTLPSSGKVEAVLAFAFAAGLAGWRMRDAFFGVCAVVLVVVYVAAKWDFVVLTMFVAG
                     VLALAAAIAMRRVWQRPTTEWLFAGIALAMPVTGWAATIFMRVFHNFATSLPVALILT
                     ATAVVLLAMGVLCRDRVLLISGTISLALVAVELQDRISLPMEAKLVTFGLAFIAAGAI
                     LARSLRRATSGFVTTPVRVAAYDEAMQIGGILAVAPHGDHAAASHDAGGPQLSDSAGP
                     TDKSFGGAGAGGGF"
     gene            complement(5580..6527)
                     /locus_tag="VNN25_26495"
     CDS             complement(5580..6527)
                     /locus_tag="VNN25_26495"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HXH95152.1"
                     /translation="MISLEPELIRLRDRPLVEGAASPAPFAQLIAVERRDIVSVYAEL
                     RLLTWLGVMIVSIGVGVLISKHLDDLGPLAIAGGIGIAAAACYAWAFWKRRQQAGLID
                     EYVLLLAALLLSADLGYIEHQFHLLGDSWQRHFLLLALLHGATAYYFQSRLVLSLSIA
                     SLAAWLGIERRSVDTIFDAPVSTAGRAFVCAAIIAGWRVIDLRLRPATTFRSLFDNAV
                     ANLAFWGALTLAAHGATRYLGCAIAIALAIASSIYARRTNSTAFVIYAWVYGTIAVDI
                     AVCSAISEPVFIMFYLLISTIGAIAGLFISNARLKKAAA"
     gene            complement(6726..7679)
                     /locus_tag="VNN25_26500"
     CDS             complement(6726..7679)
                     /locus_tag="VNN25_26500"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXH95153.1"
                     /translation="MKHYIALARPFTLLPPLLGIVSGAVCAWGSAHNPDPTRALTSSV
                     IWTIVLGSLCASLLNAANNSLNQIYDLEIDRINKPKRPLVTGEVSIRGAWIFTWIMYA
                     LGLTPTWLVVVYPYTTWHAKFFAPLPWHECIGIYLIAFISTFVYSVPSLGRTKVHPIG
                     ANLTIAIPRGCLLKVAGWTMVAHATTLEPWFIGSVFMFFLLGAASTKDFSDIEGDRAG
                     GCRTLPITFGVKRAAWMIAPFFIFPWLLMPLGAATHVLTGNGTFLTILGIVLALWGAY
                     TVWLIVRNPDELTATENHPSWRHMYLMMMAAQVGFAVAYVV"
     gene            7851..8684
                     /locus_tag="VNN25_26505"
     CDS             7851..8684
                     /locus_tag="VNN25_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXH95154.1"
                     /translation="MSRKPNFENDAAEDDGNVGSPPSRSSMNESKIALFIDFENIAIG
                     VADAKFKKFEVALLLERLLEKGKIVVKRAYSDWDKFPDYKRELHEAAIELIEIPGRVY
                     SGKNSADIRMVVDAMDMAWSKDHINIFVVASGDSDFSPLVSKLKENDKHVIGVGVKNS
                     SSDLLIDNCDEFIFYEDLVRGTKAPSNVTFKDKKQGEVFKLLIDSIKALMRENKEILW
                     GSMVKQTMQRKKPTFNEEYYGYNTFSDLLEDAQKNDIIKIKKDVKSGSYVITGFAQPT
                     P"
     gene            complement(8692..8868)
                     /locus_tag="VNN25_26510"
     CDS             complement(8692..8868)
                     /locus_tag="VNN25_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95155.1"
                     /translation="MKSSSTIKLAIAAVLAILFGGLALRAAMRLVSTATHSLFFGVVL
                     LVIAVWIFATRKKD"
     gene            8896..9216
                     /locus_tag="VNN25_26515"
     CDS             8896..9216
                     /locus_tag="VNN25_26515"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HXH95156.1"
                     /translation="MRTIALALLLTLACGKAKIDNASKPLSVPGEKVYAMHGKIVGRD
                     TKENALSIDHQAIPGYMEAMTMDYIVRGAKVESLPANGTSVDAKLHVTDEKGIWITDV
                     TKSQ"
     gene            complement(9191..9751)
                     /locus_tag="VNN25_26520"
     CDS             complement(9191..9751)
                     /locus_tag="VNN25_26520"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HXH95157.1"
                     /translation="MPGYVTSVAPLIARENLDFVILSGGRTSPRSPHSEAWVMARQLR
                     GILQSPQVVLEEHAMTTLENLIFARGLAEHHVGAVERFVVFCDRVHERKVATLGRLIL
                     GARTIVRPVDHAVAVADRLFEPFSHFIETVIAMMPRLRKYLRAAAIFTKGVSGKPPDS
                     ALPPLLSDDQPLHRRRMNLTDSSSRP"
     gene            complement(9921..>10818)
                     /locus_tag="VNN25_26525"
     CDS             complement(9921..>10818)
                     /locus_tag="VNN25_26525"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HXH95158.1"
                     /translation="VAHVEITDAGNDVVRVFHPLRRGTNIRKRGEDMHTGDVVLRDGT
                     PLHAAEVGVLAGVQKMAVRVGRRPTVAILSTGDEIIDVGDMVTPGKVVNSNSYSLAAL
                     TREAGAIPRMIGIVRDTREATIAAIESALASDFVISSGGVSVGAYDFVKDALDALGAE
                     TKFWQVAMKPGKPVVLSRLRGRLYFGLPGNPVSCMVSFLLFIAPAIRKAMGQKENLLP
                     PIVQTRVMVSLKSRGDRRNYMRVRVLARDSELVSEPMTSQGSGVSTSMVQANGLVIVE
                     TGVTSVEAGSLIATVLVGPVQS"
BASE COUNT         2239 a         3126 c         3461 g         1992 t
ORIGIN      
        1 gcgatcaggt taatcggtgc caggtttggc gagagagagc gcgcccgcct cgcttcgccg
       61 atggctttgt cgtaatcacc ctgcgccaag taggccgcgg cgagattgga tgccgtcttg
      121 gaactctctg gtttcaggtc agacgcgcgt tgaaatgatt gaacagcccc atgaacgtcg
      181 cccaaatggt atgcgcagat tccagagaag gtctgataag aaaccgcgtc tgcgtgatcc
      241 agctcgactt cacacggtat agcttttaat cggtcgcgtg ccgtgacaaa taagccagcg
      301 tcaaccaatg cttcgacgga tgcgagattc tttgccgacg gcgaaagctt agagactgaa
      361 ccgccgacgg aagcagtaag cttctgaatt tctttgagga tggcgtcgtg gccattgcga
      421 ctctcggcag ccgactgggc tatttgttcg cttagtccgg catgtcgaac tgattcgcgc
      481 gcatcgtgct cagccacgtc cagacgacgc actctgtcaa gatgaacacc gaagcgctcg
      541 attacagaag ccgctgtcag ctcgtccgtt gagacgccgg caatactcgc gacttcacag
      601 tatttggcaa taagccgctg cgttccggcg cgatctcctg gcgagatttc cgacaagtcg
      661 acgcaatcaa cccacacagt aaggattcgg tgtacatcag agaacggaac ttcggcagag
      721 gcggatgata tcgcgcgcgc cacgctgcga atcgacgaaa gcttcttgtc gataccaaga
      781 gcctttgcaa aaagcaggtt actgattaca ccatcgagaa tagagaccgg gtccatacct
      841 caccaaaagc aaggggccgc ggcagaagcc gcggcccctt cagaaaaaca agtttcgccc
      901 tacgaacacc cgctcgtact cccgcaattc aagcacttgt agcaggcccc gtttctcacc
      961 atgatcgagc cgcaatccga gcaggacggc gcgtcctgct ggtagcggaa cgtggcggtg
     1021 ttgcgggcgt tcgggccgtc ggcatccttt ggggcggcga tgatcgggga ggtgccgagg
     1081 gaggagacga atgtggcgtc gtccttggag agaggagcga cgttggggag gggtttcgga
     1141 gcgggggcag gagcttcctc gcgcacgatc acgccggcct gcgtctggct ctcgcggtcg
     1201 aggaacttgg ttgccagcca tttgaagatg tagtccatga tcgatttggt catggggatt
     1261 tcggggttct tcgtgaagcc gctgggctcg aatctcgtgt gcgtgaattt gtcgaccagg
     1321 aagttcaggg ggacgccgtg ctgcagggcc aggctcgtca tcgtggcgaa gctgtccatc
     1381 aggccggaga tcgtgctgcc ttctttggcc atggtgatga agatctcgcc cggtgtgccg
     1441 tcttcgtatt tgccgacggt gatgtagcct tcgtggccgc tgatcgagaa cttgtgcgtg
     1501 atcgactcgc gctcgtccgg cagctttctc cggacggcgg tgggggcgac gccgaggtcg
     1561 gtgagcttgt ctttgtcttt gccggtggag agcggctgcg aacgtttgca gccgtcgcgg
     1621 tagacggcaa ccgccttgag gccgagcttc cacgcttcca catacgccag ctcgatctcg
     1681 tcgatcgtgg cgttggcggg catgttgatg gtcttggaga tcgcgccgct gatgaacggc
     1741 tgcacggcgc ccatcatctt gaggtggccg atgtaatgga tcgatcttgt gccgttcttc
     1801 ggcttgaacg cgcaatcgaa gacggtgagg tgctgatcga gcaggtgtgg cgccccttcg
     1861 atcgtgtcgt tctcgtcgat gtactggatg atctcgccga tctgtttggg gtcgtatccg
     1921 aggcgcttca aggccgtggg gacggtctga ttgacgatct tcagcatgcc gccgccgacg
     1981 agcttcttgt acttgaccag ggcgatgtca ggctcgatcc cggtagtgtc gcagtccatc
     2041 atgaacgcga tggttccggt cggcgcgagg acggagatct gcgcgttgcg gtatccgtgc
     2101 tcctgcccca gcgcatacgc ttcatcccac acctgcgtcg cggcctcggc aagatcggcc
     2161 ggcagcggac gattgttgat gcgatacgcg gcacggcggt gcttgtcgat cacgcgcagg
     2221 aacggttcct ggttgatggc gtagccttcg aacggaccga ctttcgcggc gatacgtgct
     2281 gactgcgcgt acgactcgcc ggagagaatg gaagtgatgg cggcggcgta atcgcggcca
     2341 tcctcggagt catacgcaat accgcgggcc atgagcagag cgccgaggtt ggcgtaaccg
     2401 atgccgaggg ggcggaaggc gtgggagttc ttctcgatcg ccttcgtcgg atacgacgcg
     2461 aaatcgacgg tgatctcctg cgcggtgatg acaatgcgca gcgcagcacg gtaggcctcg
     2521 acgtcgaagc cggtgttctc gttgtagaag cgcatcaggt tgagcgaagc aaggttgcag
     2581 gccgaatcat cgaggaacat gtactcggag cagggattcg aggcattgat gcgcgcggtg
     2641 ttcgggcatg gatgccaatc attgacagtg gtgtcgaact gcatgccggg atcgccgcag
     2701 aggtgcgccg actcggccat cttgcgcatc agatcgcgcg ctttgtacgt gcccatgacc
     2761 gtcttcggct cggtcacggt gtaggtgttc caatcctcgc ctttctcgta cgccttcatg
     2821 aactcgtcgg tgacgcggac ggaatggttg gcgttctgga actgcacgga gtcgtaagca
     2881 ccgccgggcg cgttgaaagc accgtcgtag ccggcatcga tgagggccca ggccttcttc
     2941 tcttcttttt ccttcgactc gatgaagtcg aggatgtcgg gatggtcggc gttgaggatg
     3001 accatcttgg cggcgcggcg cgtcttgccg ccgctcttga tgacgccggc gaagctgtcg
     3061 tatcccttca tgaaggagac ggggccggat gcggtgccgc cgccggcgag aagctcgcgc
     3121 gaggagcgga tgctggagag attcgatccc gttcccgatc cgtacttgaa gagcatgccc
     3181 tcggtcttag ccaatccgag aatggagtcc atcgtgtcgt tgacggagtt gatgaagcac
     3241 gccgagcact gcggatgcgg ctcgatgccg acgttgaacc agaccggcga attgaatgcg
     3301 gccatctggt agagaaggat gtgcgtgagc tcgtcgcgga acgtgtcccc gtccgcctcg
     3361 gtgttgaagt agccgccctg acgtccccac gcggtgatcg tgcggacgac gcggccgatg
     3421 agctggcgca ccgaagtctc gcggtcaggc gagccgaggt ggccgcggaa gtacttcgac
     3481 acgacgacgt tggtggccat ctgcgaccag aacttcggca tctcgacgtc tttctgctcg
     3541 aagacgatcc cgccgctctc gttgctgatg gcagccgcac gcgtttccca ctcgcacgtg
     3601 tcatagacgt cgacgcccgc tttcgtgaag aaacgctcga cttcgatacc gccgcctttt
     3661 gtttccttct tccgggcagg cttgctgttc gagcgtggac ggaggggaga gctgggaacc
     3721 tcgatggacg tcatgactga tctccttgct tcctgtcttt cgatgcaaaa gcgcccctac
     3781 ccggggcgct ttttgagttg agtgagacgt agttaacggg acagcaaccg aacgctggcg
     3841 ggtctggcct gtggtactgg ggttttgacc ttgacggtga cctgatactc gcgaatctgc
     3901 agcgataccg accgcaacgg gctccggaca gccaaacggg aaacgtgcct caaatccttc
     3961 atcaatcctc cccgcgccat gcagttgtcg ttggtgggag acaagctacg cccggcagaa
     4021 tcggatgtca agcaaatgac cacaagatgt tgtggtcttg ttgtgcttta acacaacatg
     4081 gagcgtaagt attcgctaaa tcatatgttt agtcgataac cggaaaatgg aatggggagc
     4141 cgttctccgc tgctgttcca ccggttcccc gcaggttatc cacagtattt tgcacaggct
     4201 ttcaaggaca aatccgcaga gaatcaacgt gttttccaca tcttttgcac atgatttcca
     4261 tgcaagtttg cagcactctt tttccgcagc tttttcgatg cgaatccgaa ggaaatagca
     4321 gactcggcca ggaagaaaaa tggcgtaaat ggggcggagt gctcagcgct gcggaaaaag
     4381 gtggcttcct cggtttgact tgcttttccg gcgtgtcgtt ttgatcggag agcgattctg
     4441 cacaaccgtt gtgacacgtg gaacattaag gcggaggtgc ttccgaaagg cgtttcacgc
     4501 ggagacgcgg ggatgctgag gctccgctaa acccgatcag aatccgccgc ccgcgccggc
     4561 gccaccgaac gatttgtcgg tcggtcccgc ggagtcggag agttgagggc cgccggcgtc
     4621 gtgcgacgct gccgcatgat cgccgtgcgg agcgacggcg aggatgccgc cgatctgcat
     4681 cgcttcatcg tacgcagcaa cacggacggg cgtggtgacg aagccgctgg tggcacgtcg
     4741 caacgagcgt gcgaggatgg cgcctgcggc tatgaatgca aggccgaacg tcaccagctt
     4801 cgcctccatc ggcaggctga tgcgatcctg taactccacg gcaaccaggg cgagcgagat
     4861 ggttccggaa atgaggagta cccggtcgcg gcacaggact cccatcgcca ggagcacaac
     4921 tgcggtcgct gtgaggatca gcgcaacggg aaggctggtg gcgaagttgt ggaagacgcg
     4981 catgaagatc gtggcagccc agccggttac cggcatcgcc agcgcgatcc cggcgaaaag
     5041 ccactcggtc gtcggccgct gccagacgcg tcgcatggcg atcgctgcgg ccaatgccag
     5101 gacccctgca acgaacatcg tgaggacaac aaagtcccac ttcgccgcga catacacgac
     5161 gacgagcacg acggcgcaaa caccgaagaa ggcgtcgcgc atccgccagc cggcgagtcc
     5221 ggcggcgaac gcgaacgcga gaaccgcctc gactttgccg gagctcggca gggtgaagat
     5281 caggcagaaa agaccggcga tccagagcgt cgattccaat ccggtggaaa agaagtgatg
     5341 acggcggatc agccactccg cggctccgat cgcagcgatg ccggtgagcc attccttcgg
     5401 caacgagagg atggcaaaga atccgaagag cgccgcaagc gccacgatcg ccagaaccga
     5461 gaagacgatg cggagcatga tgccgttgtg ccgccacggc gtagccgcca cgtccgcgac
     5521 atgcagacgg cgaagcgctt ccgtcctctc ttcttcgacc gatggtcccg gtaaccagct
     5581 catgctgccg ccttcttcaa ccgcgcgttc gagatgaaca gcccggcaat cgcgccgatg
     5641 gtcgagatga ggagatagaa catgatgaag accggctcgc tgatcgcgct gcagacggcg
     5701 atgtcgaccg cgatcgtgcc gtagacccag gcgtagatga caaacgccgt cgagttagtg
     5761 cgccgcgcgt agatcgagga cgcgatggcc agcgcgatcg cgatcgcgca cccgaggtag
     5821 cgcgtggcgc cgtgcgcggc aagggtcagc gcgccccaga acgcgagatt cgcgacggcg
     5881 ttgtcgaaca gcgaacggaa cgtcgtcgcc ggtcggaggc gcagatcgat gacccgccag
     5941 ccggcgataa ttgcggcgca gacgaacgcc cgccccgcgg tcgataccgg cgcatcgaag
     6001 atcgtatcga cgcttcggcg ctcgattccc agccacgccg ccagcgatgc aatcgagagc
     6061 gagagcacga gccgcgactg aaagtagtag gcggtggcgc cgtgcaggag cgctagcaga
     6121 aggaagtgac gttgccagct gtcgccgaga agatggaact gatgctcgat gtatccgagg
     6181 tccgcgctga gcaacagggc ggcaagcagg agcacgtact catcgatcaa acccgcctgc
     6241 tgcctgcgtt tccagaacgc ccaggcatag caggcggccg cggcaatgcc aatgccgccg
     6301 gcgatggcga gcgggccgag gtcgtcgaga tgtttcgaga tgagaacacc gacgccgatg
     6361 gagacgatca tcacgcccaa ccacgtcaga aggcgcaact cagcgtagac gctgacgatg
     6421 tcgcggcgct ccacggcgat gagttgcgcg aagggcgcgg gactggccgc accttcgacg
     6481 agcggacggt cgcgaagccg aatcagctcc ggttcgaggg agatcacgtt gcgattctac
     6541 gctggatctt tttgcgctgc gcgcgttccc ggttgtcggt tgattgcgct gcgcgcgttg
     6601 tcagaaggac gcgagatcga ttgcgatgcg attgcgccgg tcggacaatc gatatcaatt
     6661 ggtttccggc ggcaacgcgc gaagcgcaat caaccgacaa ccgagaacgc gcgcagcgca
     6721 attcgttaca ccacgtacgc gaccgcgaac ccgacctgcg cggccatcat catcaggtac
     6781 atgtggcgcc atgacggatg gttctcggtt gccgtgagct cgtcgggatt gcggacgatc
     6841 agccagacgg tgtaggcgcc ccagagcgcg aggacgatgc cgaggatggt gaggaacgtg
     6901 ccgttgccgg tgaggacgtg ggtggcggcg ccgaggggca tgagcaacca ggggaagatg
     6961 aagaatggcg cgatcatcca ggctgcgcgc ttcactccga acgtaatcgg aagggtgcgg
     7021 cagcctccgg cgcggtcgcc ttcgatgtcg ctgaaatcct tcgtcgatgc ggcgccgagg
     7081 aggaagaaca tgaagacgga gccgatgaac cacggctcga gcgtcgttgc gtgcgcgacc
     7141 atcgtccagc cggccacttt caggaggcag ccgcgtggaa tggcgatcgt gaggttggct
     7201 ccgatcggat ggactttcgt cctgccgagc gacgggacgg agtagacgaa cgtcgagatg
     7261 aaggcgatga gatagatgcc gatgcactca tgccacggca gcggcgcaaa gaacttcgcg
     7321 tgccacgtgg tgtagggata gacgaccacg agccacgtcg gtgtgagccc gagcgcgtac
     7381 atgatccagg tgaagatcca cgcgccgcga atcgagactt cgccggtgac gagcggccgc
     7441 ttcggcttgt tgatgcggtc gatctcgagg tcgtagatct ggttgaggga gttgttggcg
     7501 gcgttgagca gagacgcgca gagagacccg aggacgatgg tccagatcac ggacgaggtc
     7561 agcgcgcgcg tggggtcggg attgtgcgct gagccccacg cgcagaccgc tccggagacg
     7621 atgccgagga gcggaggaag cagtgtgaag ggacgggcga gggcgatgta gtgcttcacg
     7681 gagtgggagt gtacgcgagt ggcttaaacc gtttcacgcg gaggcgcgga gatgcggaga
     7741 aaacctccgc gccctccgcg tctccgcgtg accaatcttt ctctcaatca gagcgtaccc
     7801 gtccgggaat tgaaatcccc attcggtgtt agtctgccgg agcaacaccg atgagccgca
     7861 agccaaactt cgaaaacgat gcggccgagg acgacgggaa cgtcggatcg ccgccatcga
     7921 gaagcagcat gaacgaaagc aaaatcgccc ttttcatcga cttcgagaac atcgccatcg
     7981 gcgtggccga cgccaagttc aagaaattcg aagtggccct tcttctggaa cgcctgctgg
     8041 agaaggggaa gatcgtcgtc aagcgcgcct actccgactg ggacaagttc cccgactaca
     8101 agcgcgagct gcacgaagcg gccatcgagc tgatcgagat tccaggccgc gtctattcgg
     8161 gcaagaactc cgccgacatc cgcatggtcg tcgacgccat ggacatggcc tggtcgaagg
     8221 atcacatcaa catcttcgtc gtggcgtcgg gcgactccga tttctccccg ctggtctcga
     8281 agctgaaaga gaacgacaag cacgtcatcg gcgtcggcgt gaagaattca tcgagcgatc
     8341 tgctgatcga caattgcgac gagttcatct tctacgagga cctcgtgcgc ggcacgaagg
     8401 cgccgtcgaa cgtgacgttc aaggacaaga agcagggcga ggtgttcaag cttctcatcg
     8461 actcgatcaa ggcgctgatg cgcgagaaca aagagatcct ctggggctcg atggtcaagc
     8521 agacgatgca gcgcaagaag ccgacgttca acgaggagta ctacggttac aacacgttca
     8581 gcgatctcct cgaggacgcg cagaagaacg acatcatcaa gatcaagaag gacgtgaaga
     8641 gcgggtcgta cgtgatcacg ggattcgcgc agccgacgcc gtagcgcgtt ctcaatcctt
     8701 ctttctcgtc gcgaaaatcc acaccgcgat gaccagcaac acgacgccga agaacagcga
     8761 gtgcgtcgcc gtgctcacga ggcgcatggc cgcgcgcaag gcgaggccgc cgaagagaat
     8821 ggcgagaacg gcggcgatgg ccagtttgat tgtgctcgag gacttcaact ggccgtgaga
     8881 atagactaga tcgccatgcg aacgattgct ctcgccctcc tgctcacgct cgcctgcggc
     8941 aaagcgaaga tcgacaacgc ctcaaaaccg ctctcggttc caggcgagaa ggtctacgcg
     9001 atgcacggca agatcgtggg acgcgatacg aaagagaacg cactgtccat tgatcaccag
     9061 gccatccccg gctacatgga agcgatgacg atggactaca tcgtccgagg ggcgaaagtg
     9121 gagtcgctgc cggcgaacgg aacatccgtc gatgcaaagc tccacgtgac cgacgagaag
     9181 gggatctgga tcacggacgt gacgaagagt cagtgaggtt catcctccgg cgatgcagcg
     9241 gctggtcatc ggagagcagc ggcggcaagg ccgaatcagg cggtttcccg ctaacccctt
     9301 ttgtgaagat cgccgctgcg cgcaggtatt tgcgcaggcg aggcatcatg gcgatgacgg
     9361 tctcgatgaa gtgcgagaac ggctcgaaca gacgatcggc aacagcgacg gcgtgatcga
     9421 ccggcctcac gattgtgcgt gcgccaagaa tcagccttcc cagcgtggca accttccgct
     9481 cgtgaacacg gtcacagaag acgacgaacc gctccacggc tccgacgtga tgttcggcca
     9541 gcccacgcgc gaagatcaga ttctccagcg tggtcatggc gtgttcttcg aggacaacct
     9601 gcggtgactg cagaatcccg cgaagctgcc gggccatcac ccacgcctcg gaatgcggcg
     9661 atcggggcga agtgcgccca ccggatagaa tcacgaaatc gagattctca cgggcgatga
     9721 gcggcgcaac cgacgttacg tagccgggca actcgtcgtt gagttcgtag ccacagacaa
     9781 tcccgaccga acgcatatcg ctagccccct gccgcgcgat tcacgcctaa atgcacgcta
     9841 aacgcgaggg ttacgaatga gactgggcgt tcgttgaagt gttgacagaa acagattcgc
     9901 cagtcaccgg gagctagtat tcaggattgc accggaccca cgagcactgt cgcaatcagt
     9961 gaccccgctt cgaccgacgt gactcctgtt tccacgatca cgaggccgtt ggcctgcacc
    10021 atcgatgtcg acacgcccga tccctgcgat gtcatcggct cggagaccag ctcgctgtcg
    10081 cgggccagta cccgaacgcg catgtagttg cgccggtcgc cgcgcgactt cagcgatacc
    10141 atcacgcgcg tctgcacgat cggcggaagc aggttttctt tctgccccat cgccttccgg
    10201 atcgccggcg cgatgaagag aagaaacgag accatgcacg agaccgggtt accgggaagt
    10261 ccgaagtaga gccggccgcg cagccgcgaa aggacgaccg gcttaccggg tttcatcgcc
    10321 acctgccaga acttcgtttc ggcaccaagc gcgtcgagcg catccttcac gaaatcgtag
    10381 gcgccgacag acacgccacc cgaggagatg acgaagtcag atgcgagcgc cgattcgatc
    10441 gccgcgatcg tcgcctcgcg cgtgtcgcgc acgatgccga tcatccgcgg aatcgcgccc
    10501 gcttcgcgcg tcaacgcagc aagcgagtag gaattcgagt tgacgacttt gcccggtgtc
    10561 accatatcgc cgacgtcgat gatctcgtcg ccggtcgaga ggatggccac cgttggccgg
    10621 cgcccaacgc gtaccgccat cttctgcacg cccgccagca cgccaacttc tgcagcatga
    10681 agcggcgtgc cgtcgcgcag cacgacgtca ccggtgtgca tgtcctcgcc gcgcttgcga
    10741 atattcgttc cgcgccgtag cggatggaac acgcgtacga cgtcattgcc tgcgtccgtg
    10801 atctcgacat gcgcgaca
//
LOCUS       DATLVW010000896         2355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_247467, whole genome shotgun sequence.
ACCESSION   DATLVW010000896 DATLVW010000000
VERSION     DATLVW010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247467"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /locus_tag="VNN25_26530"
     CDS             complement(<1..2217)
                     /locus_tag="VNN25_26530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH95159.1"
                     /translation="MLPSIVAITTMLLTGGVQSLPPVGALATNVRRRAATPPIHADFF
                     VATNGSDSYSGTLPAPNSGKTDGPFQSLDRARRAVASLRALQPARTGAIIVMVRDGVY
                     ALRATVELTAADSGTAQSSTIYENYPGETPVLSGGTRVTNWTNAGGNLWKVTLPSQTA
                     AFENLFYNGVRRPRPRLGGPLGAFYRIASTVYLTAPQPPAPKPDPNCAVYVDGSGWEC
                     FDRFQYAAGDPISRTWKNLAPAAQNPCHQPAGNPALSGDIEVLDFEQFTTSKLRISCV
                     DDVNRIVYMTGPTGFSQSHASEAGFITGNRYLVENVQDALTQPGQWFLDRSASPWTLS
                     YLANPGENPNVDTVIIPQLAQVLVASNLRYVTFRGLTFEHDDYVVPPAGHVSHELEPD
                     VSAAVSFQNCQNITFDSGTITQTSGTGLELIPCLNGSSPDYCVSTDLKAVTAGNVVEN
                     SAFYDLGALGMRIGEPFLQVDTDANEPQSTTVQNNVVEGYGRTIPASFGIGQGMGHDN
                     LYTHNDVYDGYHCAISTSQSIADTTVPAGIGNGNNVISFNHVHDLLQGIMNDGGSIRI
                     DGGNAVFTAAGNKILNNKIHDVSDASALDSNGYGGDGVYLDDNTGLVDVENNLVYRVS
                     GFPVYTPHGPAAPNEANIIKNNILAYGREGMISVNFPYGNGVPSAVTQVFVIRNNLFY
                     FDRSRDSSPKFWVQGGCLYSAGFPYLGYQDWNQNMYWRTDGQFASDNKAFAVQPAAG"
BASE COUNT          432 a          682 c          765 g          476 t
ORIGIN      
        1 gccggcggcg ggctgcaccg caaacgcttt gttgtcgctc gcgaattggc cgtcggtgcg
       61 ccagtacatg ttctggttcc aatcctggta gccgaggtac ggaaatcccg cgctgtagag
      121 gcatccgccc tggacccaga acttcgggga ggagtcgcgg ctgcggtcga aatagaacag
      181 gttgttcctg atgacaaaga cctgtgtgac cgcggaaggc accccgtttc cataggggaa
      241 gttcacggaa atcatcccct cgcgtccata cgccagaatg ttgttcttga tgatgttcgc
      301 ttcgttgggc gcggccggac cgtgcggcgt gtaaacaggg aagcctgaga cgcgatagac
      361 gagattgttc tccacgtcca ccaggcccgt gttgtcatcc agatagacgc catcgccgcc
      421 atatccgttg gagtcgagcg cggacgcgtc gctcacgtcg tgaatcttgt tgttcaggat
      481 cttgttgccc gcggccgtaa aaaccgcgtt tcctccatcg attcgtatcg atcccccgtc
      541 gttcatgatt ccctgcagca ggtcgtggac gtggttgaac gagatgacgt tgttgccgtt
      601 gccgatgccg gccggcacgg tcgtgtcagc gatgctctgg gacgtggaga tggcgcaatg
      661 gtagccatcg taaacgtcat tgtgcgtgta cagattgtcg tgtcccatcc cctggccgat
      721 tccgaaggat gccggaatgg tgcggccata gccctccacc acgttgttct gcacggtggt
      781 cgattgcggt tcgttggcgt cggtgtccac ctgcaagaac ggttcgccga tgcgcatccc
      841 cagagctccg aggtcgtaga aagcgctgtt ttcgacgacg ttgccggccg tcaccgcctt
      901 gagatcggtc gagacgcaat agtccggcga ggagccgttg aggcacggaa tgagctcgag
      961 accggttccc gaggtctgcg tgatggttcc agaatcgaag gtgatgttct ggcaattctg
     1021 aaaggaaacg gcggcgctga cgtcgggctc gagctcatgg gaaacgtgtc ccgccggcgg
     1081 cacgacgtaa tcgtcatgct cgaaggtcag gcctcgaaac gtcacgtagc ggaggttgga
     1141 agcgacgaga acctgcgcga gctgcggaat gatcacggta tcgacgttcg ggttctcgcc
     1201 cggattggcc aggtaagaga gcgtccaggg cgatgcggag cggtcgagaa accactggcc
     1261 cggttgggtg agcgcgtcct ggacgttttc gacgagatac cggttccccg tgatgaatcc
     1321 tgcctcgctg gcgtggcttt gcgagaagcc cgtcgggcct gtcatgtaga cgatccggtt
     1381 cacgtcatcg acacaactga tcctcagctt cgaggtggtg aactgttcga agtcgaggac
     1441 ctcgatatcg ccggagagcg caggattccc ggccggctgg tgacatgggt tctgtgcggc
     1501 aggagccaga ttcttccacg tgcgcgagat cggatcgccc gcggcgtact gaaaccggtc
     1561 gaagcattcc cacccgctgc cgtcgacgta gaccgcacaa ttcggatcag gcttcggagc
     1621 cgggggctgc ggagcggtca aatagacagt gctggcgatg cggtagaacg cgccgagagg
     1681 gccgccaagc cgcggccggg gccgcctcac gccgttgtaa aagagattct cgaacgctgc
     1741 tgtctgcgag ggaagcgtta ccttccagag gttgccgccg gcgttcgtcc agttcgtgac
     1801 gcgcgtgccc ccgctcaaga ccggggtttc tccgggatag ttctcataga tcgtcgaaga
     1861 ctgcgccgtt ccggaatccg ccgccgtgag ctccacggtg gcgcggagcg cgtagacgcc
     1921 gtcgcgaacc atcacgatga tcgcgccggt tctcgctggc tgcaaggccc gcaacgaagc
     1981 gaccgctctc cgggcgcgat cgagcgactg aaagggtccg tccgtcttcc ccgaattcgg
     2041 agccggaagg gttccgctgt agctgtcgct tccgttcgtc gcgacaaaga aatcggcgtg
     2101 aatcggaggg gtggccgccc gccgcctgac gttcgtagcc aaagcgccaa cgggagggag
     2161 ggattggacg cctcccgtca agagcatcgt cgtgatcgcg acaatcgagg gcagcatcct
     2221 ttacctgacg aacgggttga cgctttcgtt gacttcttcc tgaatttccg ggagccgaga
     2281 ttgtagcccg gccttttacc gccgtcactt gccgtcgtca gcagtccggg tcaaggagct
     2341 cgacgcgatc aacgg
//
LOCUS       DATLVW010000897        23046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_534471, whole genome shotgun sequence.
ACCESSION   DATLVW010000897 DATLVW010000000
VERSION     DATLVW010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534471"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="VNN25_26535"
     CDS             <1..2811
                     /locus_tag="VNN25_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014392937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXH95160.1"
                     /translation="EYLVARKHFTSDRSLGAGEMWIYHRSGGEGLQLTKKRTEQKDSG
                     EPAFSPDGRYVYYSDDTTPGGIYQYNKDPNTQIYVIQRLDRQTGEIEPYVTGPGGSVR
                     PTPSPDGKSLAFIRRVRYKSTIFILDLESGKETPLYDGLDRDMQETWSIHGLYPTIAW
                     TPDNKSIVFWAGGHINRIDVASKQVTNIPFHVHGSRRIEEALRFPVDVAGATYPIKML
                     RWATVSPNGKQVVFGALGYVYIRDLPNGTPHRLTKQSDHFEFYPAWSRDGKSIVYTTW
                     NDQSLGTIRIASALGGEGRVITDKPGHYIEPAFSPDGSKVVYRTTSDGYLRSPLWSRE
                     PGIYVVPSAGGKSSLVTKKGAVPHFGASNDRVFFMTFEDEDKRGLHSIGIDRTDERSH
                     LMSASATEYALSPDEKWVAFREKFNAFIAPFVRTGKSVDIGPDTKSIPVAKVTKEAGE
                     YLHWSGDGSRLYWSLGPELFSRDLKDSFAFIAGAPEKLPAAPATGTNISFSQPSNVPS
                     GRLALTGARIITMHGDDVIADGTIVIDGNRIAAIGPRASVTIPSDAKVIDAAGKTIMP
                     GIIDVHWHGSMGADEIIPQQSWINYAALAFGVTTLHDPSNNSSEIFTAAEMQRAGEIV
                     GPRIFSTGTILYGAKGSFKADIANLDDALFHLRRMKAIGAISVKSYNQPRRDQRQQII
                     EAARQTGMMVVPEGGSLFEHNMTMVVDGHTGVEHSIPVAKAYDDVTQLWSKSHSGYTP
                     TLIVGYGGLWGENYWYAKTNVWEDKRLLTFVPRRIIDSRSRRRTLAPDDEWNHFSNAS
                     LAKKLNDAGVSVQLGAHGQREGLGAHWELWMFVQGGMTPLQAIRAATLSGAHYIGMDK
                     EIGSLEAGKLADLLVLDANPLENIRNSESIRYTIANGRLFDAMTMNESGNHPRVRQPF
                     YFELPGSEAWGGATTAASDDEE"
     gene            2921..3952
                     /locus_tag="VNN25_26540"
     CDS             2921..3952
                     /locus_tag="VNN25_26540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95161.1"
                     /translation="MKKALVVVVVLAAIVYIASWFWAGDQVDTAAARTWPDGLGTLAA
                     VPGRYPPQQENDAARTLTKLAGSVAENDAVTAYVRQEIARGDLTIAEPPRIGNVSAIR
                     DLLLREPVVWERDLMQQHGGLLPRVAMHLTLMRALVASALGHARQNDAAAWDDLHAAW
                     NLTKALHAHPHLVLRVVAMSMTRAINAVAWKMPLPVPAWFGEVQALDLVHSLTETLQF
                     ENWRESKDEMFPLKPFAEKIERERTLAGNLARMTVCESSPVIKTGDRSDPIFRLDIGW
                     QRAFRYRAEREATANALRARAGKAIEERSHCSDGRWSFDGKTLRFAKPIVLPERRDTS
                     MPLTLHIRE"
     gene            complement(3958..4368)
                     /locus_tag="VNN25_26545"
     CDS             complement(3958..4368)
                     /locus_tag="VNN25_26545"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXH95162.1"
                     /translation="MIFVAVLIRSSVGTQSYMTLVAAESFYDWRELFVNDRCEDCPVR
                     VTFTPLGTAADAKANETVLDAARRAEAPLGNSCGSVGVCARCRVRVLSGTENLSPPTT
                     IEIRVASQRGFASDERLACQAVVQGDVEVTTGYW"
     gene            4346..4681
                     /locus_tag="VNN25_26550"
     CDS             4346..4681
                     /locus_tag="VNN25_26550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95163.1"
                     /translation="MSTATKITDETKLAEAIVGILDADAVRVCSDDRESIRFSIRSAG
                     MKLRSIVLRRWALRRLLSDPAGPVKVEYLQRELRRSAGQRIEYTYPRKAVVRRDLPAS
                     FPSPLAHAR"
     gene            4829..6304
                     /locus_tag="VNN25_26555"
     CDS             4829..6304
                     /locus_tag="VNN25_26555"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXH95164.1"
                     /translation="MRKLSLFFVAVLCASVAAAQTNNQKNPTWWEKYQYLVKNGEMAG
                     GAATTSVTSGSNVDVSNECGPQSETYITLNTANPKNLAGGSNEIFRDPMRAYSSSDGG
                     KTWAGTDIPLPPPIGTNGIRFGSDPTLAFDSQGNLFYGSIVVFFGGGNGINGTQMSVA
                     RSSNGGKSFPLATFFDFSGGSDHFNDKPMITADANPSSPFRDRVYIAWDAASGGSGGG
                     GVRVARSSDHGVSFVSTRADDPQGPGRAIGACPFVGTTGELYLAWNDYAANTIAFNRS
                     FDGGATWEAQRVVASKVLPFDIAVPAEFNRGALIYPACDTDRSNGSHRGRLVCSWTDL
                     AGNGTTDIFTSWSDDKGTTWSQPKAVTDSLPFAVDRFNQWLSIDPTNGVVNVSFYDTR
                     NDTTGSRYMTDIYLSRSSDGGATFAPNVRASDVSSNEHDCNGLFPCSAINYGNQQGDY
                     SGLVAYGGVAHPIWTDSRRNQETSASCSRGLMEEVFTAAVK"
     gene            6380..6850
                     /locus_tag="VNN25_26560"
     CDS             6380..6850
                     /locus_tag="VNN25_26560"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXH95165.1"
                     /translation="MAADLDALDVKILTHLLRDGRAPAQQVADSIGLSRPAVADRIAR
                     LERDGVIRGHTVVVEPAALGHFVTAFIAARGQRLDARRKKAFDVILESDEIVEAHTVA
                     GDDCFFIKVRTDSIQSLNTLVSKLTAPPLSLATRTTIVMQTHCEKVGGITLGER"
     gene            6847..7785
                     /locus_tag="VNN25_26565"
     CDS             6847..7785
                     /locus_tag="VNN25_26565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXH95166.1"
                     /translation="MKQSRTLAYAAFAVVCIVWGTTYLAIRVAVTTIPPFLLTGVRFL
                     FGGVVLFSVAKLHGDAIPRDRRVLAEVALCGVLLVAIGNLSVIWAEQWVPSGMAALFV
                     GTAPFWVSLIELTRRSGERVELRRGIGMLIGFIGVALLVTPHGAGKAFDAHFVIGALV
                     IQGGCIAWQYGTVRGKYALASIPPLMSSALQMLAGGLVVSLAGLLLGELPHFHVTPKT
                     LGALAYLAIFGSVLAYTSYVYAARHLRTTTLSLYAYVNPLVAVILGWFFLREQLTWVS
                     ITAMVIILTGVGLVQSSRGGPKRATLMNVPLPKKAA"
     gene            7795..8532
                     /locus_tag="VNN25_26570"
     CDS             7795..8532
                     /locus_tag="VNN25_26570"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HXH95167.1"
                     /translation="MLRPRFAVVLAAAALIAALTLTMHGASPKLGPHPVVVELFTSQG
                     CSSCPPADALIREISHDPALRGRVIPLSFHVDYWDQLGWRDPFSSAEWSQRQARYSAA
                     MHLSSSYTPQAVVGGRRELVGSNRSGLNAALEAASNAKPAGEIGLTVRRDANSLIATI
                     HATVPARDDLFLAVTEDDVTTRVPHGENAGRTLTNDAIVRRLIRVASGQTTVTVPIEP
                     AWRNLSAVSFAQDRNTLAIEAASAARL"
     gene            8582..8809
                     /locus_tag="VNN25_26575"
     CDS             8582..8809
                     /locus_tag="VNN25_26575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH95168.1"
                     /translation="MIKKLTRTGNSVVLVLDEPLLEELGLDENAEVELFTNGQVLVVT
                     PRRDPARERRFRKSVSKINEKYAGLFRRLSE"
     gene            complement(8904..9380)
                     /gene="msrA"
                     /locus_tag="VNN25_26580"
     CDS             complement(8904..9380)
                     /gene="msrA"
                     /locus_tag="VNN25_26580"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXH95169.1"
                     /translation="MTETATLAGGCFWGVEELVRKLPGVIDTTVGYAGGTLDNPRYED
                     VKKGKTGHAESLQIVFDPSKITFGEILDFFFRLHDPTTVNRQGNDAGTQYRSAIFYHD
                     DAQRVAAESAKQRAQPKWPRPIVTEIVPFTNFYEAEDYHQDYLQRYPDGYTCHYVR"
     gene            complement(9377..9973)
                     /gene="msrB"
                     /locus_tag="VNN25_26585"
     CDS             complement(9377..9973)
                     /gene="msrB"
                     /locus_tag="VNN25_26585"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXH95170.1"
                     /translation="MSTIWKKWLPIAAGTVIATVLVNGMMQHPVTAAPASPQVHTKGD
                     KMENYHKPSDAELKKRLTPEQYEVTQHEGTEPPFRNAYAENHAAGIYVDIVSGEPLFS
                     SLDKFESGTGWPSFTRPLEPKNVVTKTDRKFFMSRTEVRSKHADSHLGHVFDDGPAPT
                     GLRYCMNSASLRFIPVDKLEAEGYGEYLPLFGKAATKK"
     gene            complement(10112..11812)
                     /locus_tag="VNN25_26590"
     CDS             complement(10112..11812)
                     /locus_tag="VNN25_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH95171.1"
                     /translation="MSALKNRDHYPALVVGTGIAGLTASFCLAKAGIRVLLVTKAADP
                     KDCNTFWAQGGIIYEGEGDSPAKLIEDVIAAGAGLSNEEAVRFLAEEGPRVVKELLLD
                     EMHVPFSVSEEGELDLTQEGAHSLPRIIHAGDATGRAIETSLLNRVRSEPNITLVTEM
                     TAIDLLTTQHHPTDIQVRYGLTNECAGAYLLDNRTNEVFTIFSDFTILATGGIGQIYL
                     HTTNTRSAIGDGVVMAQRAGARIMNAEYVQFHPTALADKKANHYLISEALRGEGARLR
                     NQKGEYFMEKYSPVLKDLAPRDVVARAIVEELTTNKDDFVYLDLANFYHGHEPIAERF
                     PNIRKACADIGVDIERDPIPVVPAAHYFCGGILANNVGESTLERLYAIGETSCTGLHG
                     GNRLASTSLLEGLTWGYFAAASIRNKYEASTTPVEAATGPIPRRARLGDAPAVRLSYS
                     SRTPGIGAIPDALLLSIPDWRPPGSQNMEDPALILQDWTTIQHTMWNYVGIVRTYERL
                     KRSVADMRELGSRLTKYYHESTISTAIVELFHGQQMAMIVSSAALRNPVSRGAHFRRD
                     "
     gene            complement(12503..13762)
                     /locus_tag="VNN25_26595"
     CDS             complement(12503..13762)
                     /locus_tag="VNN25_26595"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH95172.1"
                     /translation="MIASVVRAGWLNLRRDRSALILSFVVPIVFFSIFAGIFGAQKSK
                     TPKTTVLIADLDRSDTSRRLIEALHRESALDIVLTPKKSTTPFDAQSAEAAVRAGDAP
                     VALIIPKSFGATQIRFDAPNSGPAFRLLSDSADPIADQVVGGLLQKTMMMSMPEMMMN
                     AGVSEVDRWSGGLTAQQKATIDQNMKGYQALNARTPATTPRTQSIIRIETTDIIGQNK
                     ANVIAALYASGIGVMFMLFTASNAGGALLEENESGTLDRILTTRLTLTQLLLGKLAYL
                     WMLGFTQMCVMFLWGALVFRLQLFQHLGGFLIMALSTALATSAFGLLLASICRTRAQL
                     GAMSTLVVLSLSAIGGSMFPRFLMPAGLQKAGLVLFNSWAIDGFTNVFWRELPLSSIA
                     LPAVVLIGWGIVFFIAARQLTKRWELA"
     gene            complement(13759..14631)
                     /locus_tag="VNN25_26600"
     CDS             complement(13759..14631)
                     /locus_tag="VNN25_26600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH95173.1"
                     /translation="MDPLQIENVVKTFASRRALDGVSLELRDAEVLGLLGPNGAGKST
                     LVRTIMGRVRPDSGTVSIFGKPAAAGDTDARAEAGVVPQEIALYPNLTSRENLSVFGR
                     YLGLRGSALAEAMERSLEWAALTDRANEPVKTLSGGMKRRLNMAAGALHRPRILLLDE
                     PTVGVDPQSRERIYQMIEALRAEGVSLIYTTHYMEEAERLCDRVAVMDAGRVIATGTK
                     DELVRNTIGTKREVVVSFADGTQVTHLIDDAAEMPRVMREAESHGSPIHDLTMKSPSL
                     EKVFLHLTGRGLRE"
     gene            14717..15673
                     /locus_tag="VNN25_26605"
     CDS             14717..15673
                     /locus_tag="VNN25_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH95174.1"
                     /translation="MRTAIVIALLASSALAQPSFAPPPAISAAPVDTSIHSTLLLPKD
                     TTHAMPVVLLIAGSGPTDRDGNSPMLPGKNNSLRMLAEGLAMNGIASLRYDKRGIGES
                     AKAMTSEADLRFETYIDDAAAFCEQLRVDKRFSSVVIAGHSEGSLIGMVAAKKCSASG
                     FISIAGIGRPAAEIIRIQLAGKLPPALATQSDAILKDLEEGKTTEKPPVELLALYRPS
                     VQPYLISWFRYDPAKSIAALSMPVMIIQGTTDIQVSVDDAKRLAAANPRAKLLIVEGM
                     NHVLKSVSSDKAKQAASYSDPALMLAPDLLMNIVDFVRKAGK"
     gene            15686..15889
                     /locus_tag="VNN25_26610"
     CDS             15686..15889
                     /locus_tag="VNN25_26610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95175.1"
                     /translation="MARPETEEAWARYFAHQLSDSERAESRTRMLADLQQAKEEGVFR
                     DLQKLRGKIKWSISLDELREDRD"
     gene            15962..16210
                     /locus_tag="VNN25_26615"
     CDS             15962..16210
                     /locus_tag="VNN25_26615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95176.1"
                     /translation="MSELKTEEPATPVSDDGEDHWAHYRSIQLTPEQKAAGRARMQKE
                     IDKARAAGVYEGLLKWQGKVHWDIDIDELREDREFDRD"
     gene            16197..16568
                     /locus_tag="VNN25_26620"
     CDS             16197..16568
                     /locus_tag="VNN25_26620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH95177.1"
                     /translation="MIAIDTSSLRRWSRDEPGIDVVRTRQVILSGEAILPPIVLAEAL
                     SSPAVEQEFIDFVLTLPLLDIEDGYWRRAGDLRRSFLREGYKAKLSDALIAQACIDKD
                     IALITHDADFRHFLRAGLKLA"
     gene            complement(16555..17202)
                     /locus_tag="VNN25_26625"
     CDS             complement(16555..17202)
                     /locus_tag="VNN25_26625"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXH95178.1"
                     /translation="MQWFHHFFSIWMQFVLDWGYLGIFIMMAFESTALPIPAEVVIPP
                     AAYWASQGRFNIALVIIAATAGSWAGSAASYWIAYKLGRPLVTRYGRFVFMSPSRIDA
                     ADRWFEAYGSGGIFVARLLPGIRHLISIPAGLFRMPFARFSLMTILGAGLFNGALAWF
                     GMRVLGDQPSLMKDPDALRNALSSKSMWIIGFAVVVAVLYGVMVWMQKRAETTRA"
     gene            17245..18873
                     /locus_tag="VNN25_26630"
     CDS             17245..18873
                     /locus_tag="VNN25_26630"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HXH95179.1"
                     /translation="MQPHDYDVIILGGAFSGASTAILLRRDHPQLRVLIVEKAEAFDA
                     KVGEATTEMSAMFLTRRLAMWHHLESEQLPKEGLRYWFTNDKVTGHGNASEAGGYLRS
                     AVPAFQLRRDALDEYLLATAVREGAELLRPAKVVDVDLQDFNNTITVMLREDAERPPA
                     SVAPWEAADSSEASSKSNGAEPSRRTLTCRWLLDATGRVNFLGRRLGLIERNDEHPIA
                     AIWCRWNGLRHIDDIAARDGMLATRNISSRRLATNHYTGFGYWIWVIPLGNGETSIGV
                     VFDKRLIDLHHSKDRATDFIAFLKGIPSLAELLVEATPRLEDLRFYSHLPYFSKQYMG
                     NGWALLGDAASFLDPYYSPGLDHASFTAEATAEIVGLHAKGEDIAARITEHNDAFTRS
                     YTRFFRSIFKDKYYYMGEVDLLGAAMILDTAHYYIFVVIPAYRFAKKFQWMPVLGPKP
                     AFFSYHFIKFYHRRFKAIALARRAAGEEGVRNNGRRIKAYFDLNLSPFHMFLRGVKLW
                     AFAEADNVRLQVRRLFGRRAATTASRAEGAAAQG"
     gene            18870..19196
                     /locus_tag="VNN25_26635"
     CDS             18870..19196
                     /locus_tag="VNN25_26635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95180.1"
                     /translation="MSRRVAIVFDREFGDRLGDLAMRMPVWIVESNTNRPAVAAAWNR
                     ASEWPHISVTVFRPPDDLRLLLSQIGHPQRVDVIGLPLTDEARDTLIAAGFTTIAETS
                     DGFRAK"
     gene            complement(19203..20648)
                     /locus_tag="VNN25_26640"
     CDS             complement(19203..20648)
                     /locus_tag="VNN25_26640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXH95181.1"
                     /translation="MKILPIASNPQDLSAPAVDVPRGRLYPPSPTHMLERFYALAVAA
                     PETVALIDGATGRITTRAQLAERIDELGARFAGAGFRERDVVAIQLPNSVDLVAGFGA
                     ILKQNLVAILIDRDATETEVARILGHFSARGLVYRNGDEVLISTRTAQRAAIPENARL
                     IKLTSGSTGLPKGIVASDANLLADCLNICVTMNIREGDINLGAIPMSHSYGFSNLVTP
                     LLVQGTPVVISNDYLPQSVMNLCNQFACTVAPLIPMVFDHLVTAAAGEFSTVRTFLSA
                     GAPLPAVVSRKFRGRFGTPIHTFYGCSECGGITYDREGASVERGTVGQAMENVQLSSA
                     RGRLTVRGSNVSLGYLHDANTFERFENERFLADDLIELCDGGEIAITGRASDLINTAS
                     KKVNPREVEQVLLQIDGVRQAKVYGEPAGARGEVVAAAVVASPDVTREQIRTWCRDHL
                     SPHKVPRIIKLIESIPLDERGKVKRAALAEL"
     gene            20681..20953
                     /locus_tag="VNN25_26645"
     CDS             20681..20953
                     /locus_tag="VNN25_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXH95182.1"
                     /translation="MNDLKRKIKELLIERLKFEDMTPDDIKDDEPLFAGGLGLDSIDA
                     LEIVVMLESEFGIKVKNESAARDNFQSVAALAQFVEQRLAEQQAQA"
     gene            20950..21330
                     /locus_tag="VNN25_26650"
     CDS             20950..21330
                     /locus_tag="VNN25_26650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95183.1"
                     /translation="MSWLASLPHQIPFRAASAATRTGDQTIEGVFVCTVNDAMPAGVM
                     AVEAMAQLGGGLAFADSSSHAFLTGIDGCEVDRFIETGDVIRFVVTLDASFGGTFRFS
                     GRGLVDGVEVIRSRFYLASQDAQS"
     gene            21317..22540
                     /locus_tag="VNN25_26655"
     CDS             21317..22540
                     /locus_tag="VNN25_26655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXH95184.1"
                     /translation="MHKADTSTRLAVTGIGIICSIGRNQVEVWRSITESRSGIGGLTK
                     FPGDTFPTDIAAEVDDDLYDALEIDGREAKRMSRSDLLAVIAASEAMAQSDRGARAPL
                     PRDRAVVSTGTSTGGLLEGEDFYFDRLVHGRRRVATSRVLQQPTSGPSDAVARALNLG
                     GGVLSNATACASAGAAIGVAADLLRSRHVDVAVAGGSDALCRLTYSGFNVLQAVDEKP
                     CKPFAAERKGITLGEGAAYLVIERWDDAVARGASILAELCGYGATCDAHHPTAPHEEG
                     RGAEAAMRGAIAEAGDDPRIDYINAHGTGTLLNDSAETKAIIAAVGETVPVSSSKSYF
                     GHTLGAAGGIEAVITVLTLQHQLAPPTLRLDMPAADCTLDYIPHTPRPMPMTNVLSNT
                     FGFGGSNVSLLFRRA"
     gene            22560..>23046
                     /locus_tag="VNN25_26660"
     CDS             22560..>23046
                     /locus_tag="VNN25_26660"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXH95185.1"
                     /translation="MNRVAITGIGIVAPEAVGIEPFRAMLDAGTTAVQPIDRFDTEGL
                     AAHTAALVRDFKARDYIPAMKMRRMNTLSRYGVAAARMAIDDSAAKPASGSGVAIGTA
                     FGPVQTSVDYMQEYVSRGPALAPPQLFAESVANAPGSHIAIEFDLRGFNITMTQRESS
                     LL"
BASE COUNT         4326 a         7082 c         7241 g         4397 t
ORIGIN      
        1 gaatacctcg tcgcccgcaa gcacttcacc tccgaccgtt ccctcggcgc gggcgagatg
       61 tggatctatc accgcagcgg cggcgagggt ctgcaactca cgaaaaagcg aaccgagcag
      121 aaggacagcg gcgagcccgc gttttcgccc gacggccgct acgtttatta cagcgacgac
      181 acgacgccgg gcggaatcta ccaatacaac aaagatccga acacgcagat ctatgtcatc
      241 cagcgtctcg accggcagac cggcgagatc gagccgtacg tcacgggccc cggcggctct
      301 gtccggccga cgccgtcgcc tgacggcaaa tcgctcgcgt tcatccggcg cgttcgctac
      361 aagtcgacga tcttcatcct cgatctcgaa tcgggcaaag agacgccgct gtacgacggc
      421 ctcgatcgcg acatgcagga gacctggtcc attcacggcc tctatccgac gatagcatgg
      481 acgccggaca acaaatcgat tgtcttctgg gccggtggtc acatcaaccg catcgacgtc
      541 gcctcgaagc aggtcacgaa catcccgttc cacgtccacg ggagccggcg catcgaggag
      601 gcgcttcgct ttccggtcga cgttgcgggc gcgacgtatc cgatcaagat gcttcgctgg
      661 gcaacggttt cgccgaacgg caaacaggtt gtcttcggtg ccctcggtta cgtctacatc
      721 cgcgatcttc cgaacggcac gccgcatcgc ctgacgaagc agagcgacca cttcgagttc
      781 tacccggcct ggtctcgcga cggaaaatcg atcgtctaca cgacatggaa cgatcagtca
      841 ctggggacga ttcgaatcgc ctcggcgctc ggcggcgaag gccgcgtgat caccgacaag
      901 ccaggccact acatcgagcc cgctttctcg cccgacggat cgaaagtcgt ttaccgcacg
      961 accagtgatg gctatctccg ctcgccgctg tggtcgcgcg agcccggcat ctatgtcgtg
     1021 ccgtcggccg gcggcaaatc gtcgctcgtc accaagaaag gcgcggttcc gcattttggc
     1081 gcctcgaacg accgcgtctt cttcatgacc ttcgaggatg aggacaagcg cggcctgcac
     1141 tcgatcggca tcgaccgcac cgacgagcgg tcgcatctga tgtccgcgtc cgcgactgag
     1201 tacgcgctct cgcccgacga gaagtgggtg gctttccgcg agaagttcaa cgcgttcatc
     1261 gcgccgttcg tgcgaaccgg caagtcggtc gacatcggcc cggatacgaa gtcgatcccg
     1321 gtcgctaagg taacgaaaga agcgggcgag tatctgcact ggtccggcga cggatcgcgg
     1381 ctctactggt cgctcggccc cgagcttttc tcgcgcgact tgaaggattc cttcgccttc
     1441 atcgccggtg cgcccgagaa attgcccgcc gcgcctgcga ccggcaccaa catcagtttt
     1501 tcgcaaccct ccaacgtccc ctcgggacgc ctcgcactta ccggcgctcg catcatcacg
     1561 atgcacggcg atgacgtgat cgctgacggc acgatcgtca tcgacggcaa tcgcatcgca
     1621 gcaatcgggc cgcgcgcgag cgtgacgatt ccttccgatg cgaaagtgat cgacgccgcg
     1681 ggcaagacga tcatgcctgg catcatcgat gtccattggc acggctcgat gggcgcggac
     1741 gagatcattc cgcaacagag ctggatcaac tacgcagcgc tcgcgttcgg cgtgacgacg
     1801 ctccacgatc cttccaacaa ctcgtcggag atctttaccg cggcggaaat gcagcgcgcc
     1861 ggagagatcg tcggcccgcg catcttctcg accggcacga tcctctacgg cgcgaagggc
     1921 tcgttcaaag ccgacatcgc gaacctcgat gatgcgctct tccacttgcg ccggatgaag
     1981 gcgatcggcg ccatcagcgt gaagagctac aaccagccgc gtcgcgatca acggcaacag
     2041 atcatcgagg ccgcgcgcca gaccgggatg atggtcgtgc ccgagggtgg gtcgctgttc
     2101 gagcacaaca tgacgatggt ggtcgacgga cacaccggcg tcgagcactc catccccgtg
     2161 gccaaggcgt acgacgacgt cacgcaactc tggtcgaagt cgcacagcgg ctatacgccg
     2221 acgctgatcg tcggctacgg cggactgtgg ggcgagaatt actggtacgc gaaaacgaac
     2281 gtgtgggaag acaagcggct gctgacgttc gtgccgcgac ggatcatcga ctcgcgctca
     2341 cgccgccgca cgctcgcacc cgacgacgag tggaatcact tcagcaacgc gtctctcgca
     2401 aaaaaactca acgacgccgg cgtgtcggtc caactcggcg cacacggtca gcgcgaaggc
     2461 ctcggcgcgc actgggagct ctggatgttc gtgcagggcg gcatgacgcc gctccaggcc
     2521 atccgcgccg caacgctcag cggcgcgcat tacatcggga tggacaaaga gatcggttcg
     2581 ctcgaagccg gcaagctcgc cgatcttctt gtcctcgacg ccaatccgct tgagaacatc
     2641 cgcaactccg aatcgatccg ctacacgatc gcgaacggca gactctttga tgcgatgacg
     2701 atgaacgagt ccggcaatca tccgcgcgtt cgccagccgt tctacttcga gttgcccggc
     2761 agcgaggcat ggggaggggc gacaacggcg gcgagcgacg atgaggagtg aagcgaagcc
     2821 tggcggcgtc ctcgccgccg acgttcgttt ttgcgtaacg tcgtgccgaa gcgcgtcgat
     2881 tttgctggca atgagcgtct cggcgagcga tccttaatcc gtgaagaaag cattggttgt
     2941 ggttgtcgtg ctagccgcga tcgtctatat cgcctcgtgg ttctgggcgg gagaccaagt
     3001 cgataccgcg gcagcacgga cgtggccgga cggacttgga acgctcgccg ccgttcccgg
     3061 acggtatcca ccgcagcagg agaatgacgc cgcgcggacg ctgacaaagc tcgctggctc
     3121 ggtcgccgaa aacgacgctg tgacggctta cgttcgacag gagatcgcgc gcggggatct
     3181 gaccatcgcc gagccgccgc ggatcgggaa cgtctccgcg attcgcgacc tcttgctgcg
     3241 agagccggtc gtgtgggagc gcgacctcat gcagcagcac ggcggtcttc tgccgagggt
     3301 cgcgatgcac ctgacgctta tgcgcgctct ggtcgcgagc gccctcgggc acgcacgaca
     3361 gaacgatgcg gccgcgtggg atgacctgca cgcggcgtgg aacctgacga aagcgctcca
     3421 cgcacatccg cacctggtct tgcgtgtcgt ggcgatgtcg atgacgcgcg ccatcaacgc
     3481 cgtggcgtgg aagatgccgc ttccggtgcc ggcgtggttc ggcgaggtgc aggcgctcga
     3541 cctcgtgcac tccttgaccg agacattgca attcgagaat tggcgggagt cgaaggacga
     3601 aatgtttcct ctcaagccgt tcgccgagaa gatcgagcgc gagcgcactc tcgcgggtaa
     3661 cctcgcgcga atgaccgtct gcgagtccag ccctgtgatc aagacaggcg atcgctcgga
     3721 tccgatcttc aggctcgaca tcggctggca acgtgccttt cggtaccgcg cggagcgcga
     3781 ggcaaccgcg aacgctctgc gcgcacgcgc cggaaaggcg atcgaagaga ggtcgcactg
     3841 cagtgatggc cggtggagtt tcgacgggaa aacactccgg ttcgcgaagc cgatcgtttt
     3901 gcccgagcgg cgcgatacgt cgatgccgct gacgctgcac attcgcgagt gacgcgctca
     3961 ccaatacccg gtcgtcactt ccacatcgcc ctgcaccacc gcctgacacg ccaatcgctc
     4021 atcgctcgca aatccgcgct gggacgctac ccgtatttcg atcgtcgtgg gggggctcag
     4081 gttttcggtg ccggacagaa cccgaacgcg gcagcgcgcg cagacgccga cgctgccgca
     4141 ggaattgccg aggggtgcct cggcgcgacg ggctgcatcg agaacggtct cgtttgcctt
     4201 cgcgtcagcg gcggtaccga ggggggtgaa agtcacacgc actggacaat cttcgcaccg
     4261 gtcgttcaca aagagttcgc gccagtcgta gaaactttcg gccgcgacaa gcgtcatata
     4321 gctttgagta ccaacggagg aacgaatgag cacggcgacg aagatcacgg acgagacgaa
     4381 actggcagag gcaatcgtgg ggattctcga tgcggacgct gtacgcgttt gctcggacga
     4441 ccgcgagtcg atccgctttt ccattcgttc agccgggatg aagctgcgct cgatcgtcct
     4501 tcgccggtgg gccctgcgcc gcctgctcag cgatccggcc ggccctgtga aggtcgagta
     4561 cctccagcgt gagctgcgcc gttccgcagg acagcggatc gagtacacgt atccgaggaa
     4621 agcggtggtg cggcgcgacc tgccggcatc gttcccgtcg ccgctggcgc acgcacgttg
     4681 atgtgcagaa cttgatccgt aggaggggtc aatcaacgca aggttgtcca caaatggacc
     4741 cacgcgcgag ctgtaagtgc ttgattctgc gttgatccga tctggccctt ctcttgcgca
     4801 ggcatgcgcg cgaaaggagt tccccttcat gcgcaagctc tctctctttt tcgtggctgt
     4861 cctctgcgcc tcagtggcgg cggcacagac caacaaccag aagaatccca cgtggtggga
     4921 gaagtatcag tacctcgtca agaacggcga aatggccggc ggcgctgcca cgacctcggt
     4981 gacgtccgga agcaacgtcg acgtctcgaa cgaatgcggt ccgcaaagcg agacgtacat
     5041 cacgctcaat acggcgaacc ccaagaacct cgccggcgga tcgaacgaga tctttcgcga
     5101 tccgatgcgc gcctattcgt cctcggacgg cgggaagacc tgggcgggca ccgatatccc
     5161 tctgcctccg ccgatcggca cgaacggaat ccgcttcgga tccgatccca cgcttgcctt
     5221 tgattcgcag ggcaatctct tctacggctc catcgtcgtg ttctttggcg gcgggaacgg
     5281 aatcaacggt acgcagatgt cggtggcgcg gtcgagcaat ggcgggaagt cgtttccgct
     5341 tgcgaccttc ttcgatttct ccggcggaag cgatcatttc aacgataagc cgatgatcac
     5401 ggccgacgcg aacccgtcgt cgccgttccg agaccgcgtc tacatcgcgt gggatgcggc
     5461 gagcggcggg tcgggtggcg gcggtgtccg ggtggctcgt tcgagcgacc acggcgtctc
     5521 gttcgtgtcc acgcgtgctg acgatccgca aggtcccgga cgcgccatcg gcgcctgccc
     5581 gttcgtcggt acgaccggcg agctctacct cgcctggaac gattacgccg cgaacacgat
     5641 tgcattcaat cgctcgttcg atgggggcgc tacgtgggag gcgcagcgcg tcgtcgcctc
     5701 gaaagtgctt ccgttcgaca tcgctgttcc ggccgagttc aaccgcggcg cgctcatcta
     5761 tccggcatgc gacaccgacc ggagcaacgg ctcgcatcgc ggacgcctcg tttgttcgtg
     5821 gaccgatctc gccggaaacg ggacgaccga catcttcacg tcgtggtccg acgacaaggg
     5881 gactacctgg tcgcagccga aggctgtcac cgactcgttg ccatttgccg tcgaccgctt
     5941 caatcaatgg ctctcgatcg atccgaccaa cggcgtcgta aacgtctcgt tttatgacac
     6001 gcgcaatgac accacgggat cccgctatat gaccgacatc tacctgtccc gttcgtccga
     6061 tggcggtgcg acgttcgctc cgaacgtgcg cgcgtccgat gtcagctcca acgagcacga
     6121 ttgcaatggt ctcttccctt gctcggcgat caactacggc aaccagcagg gcgactactc
     6181 gggactcgtc gcctacggtg gcgtcgccca tccgatctgg accgacagcc gccgcaatca
     6241 ggagacctcc gcgagttgct cgcgagggct gatggaagaa gtgttcaccg ccgccgtcaa
     6301 gtaaccctgc gaagtagtac gccgacggat cgtcggcgta ctacttgatc ttccgtcgat
     6361 tcgggcgtac gttagcggca tggccgctga tctggatgct ctcgacgtca agattctgac
     6421 gcaccttctt cgtgacggcc gcgctccggc gcagcaggtg gcggacagta tcggcctctc
     6481 gcgtccggca gttgccgacc ggattgcacg cctcgagcgc gacggtgtca ttcgcggaca
     6541 cacagtcgtc gtcgagccgg ccgcactcgg ccacttcgtc acggccttca tcgccgcacg
     6601 cggtcagagg ctcgacgcga ggcgaaagaa agcgttcgac gtcattctcg agagcgacga
     6661 gatcgtcgag gcgcacacgg tcgctggcga cgattgcttc ttcatcaagg tccgcaccga
     6721 ctcgattcaa tcgctgaata cgctcgtctc gaaactgacg gcccctccgc tgtcgctggc
     6781 gacgcgcacg acgatcgtga tgcagacaca ttgcgaaaag gtgggcggca tcacgttggg
     6841 agagcgatga aacagagccg cacgcttgcc tacgccgcgt ttgcggtggt ctgcatcgtc
     6901 tgggggacga cgtatctggc gatccgcgtg gccgtgacga cgatcccgcc gtttctgctc
     6961 accggcgtgc gctttctctt cggcggggta gtgctattca gcgtggcaaa gctgcacggc
     7021 gatgccatcc cgcgcgaccg tcgtgtgctt gccgaggtcg cgctctgcgg cgtgttgctg
     7081 gtggcgattg gcaacctgag cgtcatctgg gctgagcagt gggtgccaag cgggatggct
     7141 gcgcttttcg tcggcacggc acctttctgg gtctcactga tcgaactcac ccggcgaagt
     7201 ggagagcggg tggaactgcg ccgcggcatc gggatgctga tcggattcat cggtgtcgca
     7261 ctgctcgtca cgccgcatgg cgcgggcaag gcattcgatg cgcactttgt gatcggcgcg
     7321 ctggtcatcc agggtggttg tatcgcctgg cagtacggca ccgtgcgcgg aaagtacgca
     7381 ctggccagca tcccacccct gatgtcgtcg gcgctgcaga tgctcgcggg tggcttggtg
     7441 gtctccctgg ccggccttct cctcggcgag cttccgcact ttcacgtcac gccgaaaact
     7501 ttgggtgcgc ttgcctatct cgcgatcttc ggatcggtgc tggcctacac gtcgtacgtt
     7561 tacgccgcgc gccatctccg tacgaccacg ctctccctct atgcctacgt caacccgctc
     7621 gtcgccgtga tcctcggatg gttcttcctg cgcgagcaat tgacttgggt gtcgatcacc
     7681 gcgatggtga tcattctcac cggcgtgggg ctggtgcaga gctcgcgcgg tggtccaaag
     7741 agagcgacgc tgatgaacgt tccgctgccg aagaaggcgg cgtagactcg tgtcgtgcta
     7801 cgtcctcgat tcgcagtcgt tctggccgcc gcagcgctaa tcgccgcact cacgctgacg
     7861 atgcacggcg cctcgccgaa gctcgggcct catccggtcg tcgtcgagct cttcacgtcg
     7921 cagggatgct ctagctgtcc gcccgcggat gcgttgattc gcgagatctc tcacgacccg
     7981 gcgctgcgtg gccgcgtcat tccactttcg tttcatgtcg actactggga ccaactcggc
     8041 tggcgcgatc ccttttcgtc cgccgagtgg tcgcagcggc aggcgcgcta ctccgccgcc
     8101 atgcatcttt catcgagtta tacgccgcaa gccgtcgtcg gcggcaggcg cgagctggtc
     8161 ggatcgaatc gttcggggct caacgccgcg ctcgaagctg cgtcgaacgc taaaccggcc
     8221 ggcgagatcg gcctgacggt tcgtcgggat gccaattcac tcatcgccac gattcatgcg
     8281 acagttcctg cccgcgacga cctctttctc gccgtgaccg aggacgatgt cacgacgagg
     8341 gttccgcacg gagaaaatgc cggacgaacg ctcacgaacg acgcgatcgt gcgccggctg
     8401 atccgagttg cgtccggaca gaccaccgtc acggttccga tcgagccggc atggcgcaat
     8461 ttgagcgctg tctcatttgc gcaggatcgg aacacgctgg ccatcgaggc agcgtcggcg
     8521 gcgcgccttt gaccttgccg tccggatcgc gtacgtacgg gaatgatatt ttgcacggac
     8581 gatgatcaag aagctcacgc ggactggaaa cagcgttgtc ctcgttttgg acgagccgct
     8641 gctcgaagag cttggcctcg atgaaaacgc ggaagtcgag ttgttcacga acgggcaggt
     8701 gctcgttgtg acgccgaggc gcgatcctgc acgcgaacgc aggttccgca agtccgtttc
     8761 aaagatcaat gaaaagtatg cgggtttgtt ccgccggttg agtgagtagt gaagggtctc
     8821 tttctcgcta cattagaaga gctcccttgg cgtgatgaac accggctcat atcgatcgcg
     8881 ccgatgacga ccagacgaat tgtctacctc acgtagtggc acgtgtaccc atccggatac
     8941 cgctgcaagt agtcctggtg atagtcctcg gcttcataga aattcgtgaa cgggacgatc
     9001 tcagtcacga tcggacgggg ccactttggt tgcgcgcgct gcttcgcgga ctcggcggcg
     9061 acgcgctggg cgtcgtcgtg atagaaaatc gcggagcggt attgcgtccc ggcgtcgttg
     9121 ccctggcgat tgaccgtggt cggatcgtgg aggcggaaga agaagtcgag gatctcgccg
     9181 aaggtgatct tcgaggggtc gaagacgatc tgcagcgact ccgcgtggcc ggtcttgcct
     9241 ttcttaacgt cctcatagcg cgggttgtcg agggtgccgc cggcgtaacc gacagtcgtg
     9301 tcgatcacgc cgggcagttt gcgtacgagc tcttcgacgc cccagaagca gccgccggcg
     9361 agcgttgcgg tctcggtcat ttctttgtcg ccgcctttcc gaacagcggg aggtattcgc
     9421 cgtagccctc ggcttcgagc ttatcgaccg ggatgaagcg gagtgacgcg gagttcatgc
     9481 agtagcgcag gccggtcggc gccggtccgt cgtcgaacac gtggccgagg tgcgagtcgg
     9541 cgtgtttgga gcggacttcg gtgcgggaca tgaagaactt gcgatcggtt tttgtcacga
     9601 cgttcttcgg ctcgagcgga cgggtgaagc tgggccagcc agtgccggac tcgaacttgt
     9661 cgagcgaact gaagagaggc tcgccactga cgatgtcgac gtagatgccg gctgcatgat
     9721 tctcagcgta cgcattgcgg aacggcggct cggttccctc atgctgggtc acttcgtact
     9781 gctcgggcgt gaggcgcttc ttcagctccg catcggaagg cttgtggtag ttctccatct
     9841 tgtctccttt ggtgtggacc tggggactgg cgggggcggc ggtgaccggg tgttgcatca
     9901 tgccgttgac gaggaccgta gcgatgaccg tccctgcggc gatcggcagc cactttttcc
     9961 atatcgtgct catcggatgc tccttgtgca cagtacgaaa cccgagggga tggcggattc
    10021 gttcccgacg taggtcggac tctccagtcc gactgcacgt gatgtcggac tagagagtcc
    10081 gacctacgta gactagagag tccgacctac gtcaatcgcg ccggaaatgg gccccgcgcg
    10141 agacaggatt ccgcagcgcc gcgctcgaca cgatcatggc catctgctgg ccgtggaaga
    10201 gctcgacgat cgcagtgctg atcgtcgact cgtggtagta cttcgtcagg cggctaccga
    10261 gctcgcgcat gtccgccacg ctccgtttca ggcgctcgta cgtgcgcacg atccctacgt
    10321 agttccacat ggtgtgctgg atcgtggtcc agtcctgaag gatcagcgcg gggtcttcca
    10381 tgttctggct gccgggaggc cgccagtcgg ggatcgagag gaggagggcg tcggggatcg
    10441 ctccgatgcc gggggtgcgc gacgaatacg acagacgaac tgcaggcgca tcgccgaggc
    10501 gggcacggcg ggggatgggt ccggttgcgg cctccactgg cgtggtggac gcttcgtact
    10561 tgttgcggat cgaagcggcg gcgaagtagc cccaagtcaa gccttccagg agtgacgttg
    10621 atgccagacg attgccgccg tgaaggccgg tacacgaagt ctcgccgatc gcgtagagcc
    10681 gctcaagggt cgactccccg acgttattgg cgaggatgcc tccgcagaag tagtgcgccg
    10741 caggaacgac gggaattgga tcgcgttcga tgtcgacgcc gatatccgcg caggctttgc
    10801 ggatgttcgg gaagcgctca gcgatcggtt cgtgtccatg gtagaagttg gcgaggtcga
    10861 gatagacgaa atcgtccttg ttcgtcgtca gctcttccac gatggcgcgc gccacgacgt
    10921 cgcggggagc gagatccttc agtaccggtg agtacttctc catgaagtac tcacccttct
    10981 gattgcgcag tctcgccccc tcgccgcgca acgcttcgga gataaggtag tgattggcct
    11041 tcttgtcggc cagcgcggtg gggtggaact gcacgtactc ggcattcatg attcgtgcgc
    11101 cggcgcgttg ggccatcacg acgccgtcgc cgatggcgct gcgggtgttc gtcgtgtgca
    11161 ggtagatctg cccgatgccg cccgtcgcca ggatcgtgaa atcgctgaag atcgtgaaga
    11221 cctcgtttgt ccggttgtcg agcaggtacg cgcccgcgca ttcgttggtg aggccgtagc
    11281 gaacctgaat gtcggtcgga tgatgttgcg tcgtcagcag atcgatggcc gtcatttcgg
    11341 taaccagcgt gatgttcggc tcgctgcgga cgcggttgag gagcgacgtc tcgatggcgc
    11401 ggccggtggc gtcgccggcg tggatgatgc gcggcagcga gtgcgccccc tcctgcgtca
    11461 gatcgagctc gccctcttcg ctgacggaga aagggacgtg catctcatcg agcagcagtt
    11521 ccttgacgac gcgcggcccc tcctcggcga ggaagcgcac tgcttcctca ttcgagagtc
    11581 cggcaccggc ggcaatgacg tcctcgatga gcttcgcggg tgagtcgccc tcgccctcgt
    11641 agatgatccc gccctgagcc cagaacgtgt tgcagtcctt gggatcggct gctttcgtga
    11701 cgagcaggac acggatgccg gccttcgcca gacagaacga ggccgtcagt ccggcgatcc
    11761 cggtgcctac gacgagggca ggatagtgat cgcggttctt gagcgcagac acgcggcgca
    11821 ttgtagtggt caatcggcgg ctgcgccgcg ttgtcggttg tcggttatcg ggtggtcctg
    11881 cgttgccgga ctgcttgcgg attcaagagc cggtcgtcgc acatctgatg tttggatcac
    11941 caagccgtcg tcatcgggtt ctccaagcgc ccgcaagagt aaggcggcat ttttgttgtt
    12001 cagcgacacc cttgcgggaa taactgaggt cagttgccac agcagttcgt cgatttcggg
    12061 tcattttcca ccaaaatcag gtgattctga ccctccgact gcgactcggt cgattttaaa
    12121 tttgaatacc gactcaaaaa atatgagtcc gtactcgaga aacatgagta ccgactcacc
    12181 gaacttgagt acggactcac cgaacttgag taccgactca ccgaacttga gtaccgactc
    12241 accgaacttg aatacggact caacgaacct gagtacggac tcaagagttg ggagtccgga
    12301 ctcacgtcac tcgagtccgt actcaaaagt tgtgagtccg gactcacgtc actcgagtac
    12361 cgactcacga gatttgagta ccgactcacc ggacgtgagt acggacccag atgatgtgag
    12421 taccgactca tcggttttga gtcggtactc gatgggtctg actctgccct cacgggacga
    12481 agatggcgac gtcagttgac gctcacgcta actcccaccg tttcgtcagt tgccgcgccg
    12541 cgatgaagaa cacgatcccc catccgatca gaacgacggc tggcaacgcg atcgaactca
    12601 aaggcagctc ccgccagaag acgttcgtga agccgtcgat tgcccaagaa ttgaagagca
    12661 ccagtcccgc tttctgcaat ccggcgggca tgaggaagcg cgggaacatc gagcctccga
    12721 ttgctgacaa cgacagcacg acgagcgtcg acatcgcgcc gagttgcgcc cgcgtgcggc
    12781 agatcgaggc gagaagaagg ccgaatgccg acgttgccag cgccgtcgag agggccatga
    12841 tgaggaagcc gccgaggtgc tggaagagtt gcagccggaa cacgagcgct ccccagagaa
    12901 acatcacaca catctgcgtg aaaccgagca tccagaggta ggcgagcttg ccgaggagga
    12961 gttgggtcag ggtgaggcgc gtggtgagaa tgcggtcgag tgtgccgctc tcgttctctt
    13021 cgagcagcgc gccgcccgcg ttcgatgccg tgaagagcat gaacatgacg ccgatgcccg
    13081 aggcgtagag agcggcaatc acgttcgctt tgttctggcc gatgatgtcg gtcgtctcga
    13141 tgcggatgat cgattgcgta cgtggcgtcg ttgcaggcgt ccgcgcgttg agagcctgat
    13201 agcccttcat gttctgatcg atcgttgcct tctgctgcgc cgtcaggccg ccgctccatc
    13261 gatcgacttc gctgacgccg gcgttcatca tcatctcggg catcgacatc atcatcgtct
    13321 tctgcagcag ccctccgacg acctggtccg cgatgggatc cgcggagtcg gagagcaggc
    13381 gaaatgccgg gccgctgttc ggcgcgtcga aacgaatctg cgtcgcgccg aagcttttcg
    13441 ggatgatcag cgctacaggc gcatctcccg ctcgcacggc cgcttccgct gactgcgcat
    13501 cgaacggtgt cgtgctcttc ttcggtgtca ggacgatatc gagtgccgat tcgcggtgca
    13561 gcgcttcgat gagccgtcgc gacgtgtcgc tgcgatcgag atcggcgatc agcacggtcg
    13621 tcttcggcgt tttcgacttc tgcgcgccga agattcctgc gaagatcgag aagaagacga
    13681 tcggcacgac gaaactgagg atgagcgccg agcggtcccg gcgcagattg agccatccgg
    13741 ctcggacgac agaggcaatc attcgcgtaa accccttccg gtcaggtgaa gaaagacttt
    13801 ctcgagcgat ggcgacttca tcgtcaggtc gtgaatcggg cttccgtgag attccgcttc
    13861 gcgcatgacg cgcggcattt ccgcggcgtc atcgatgagg tgcgtgacct gggtgccgtc
    13921 ggcaaacgag acgacgacct cgcgtttggt gccgatcgtg ttgcgcacca gctcatcctt
    13981 cgtgccggtg gcgatgacgc ggccggcatc catgacggcg acacggtcgc aaagccgctc
    14041 cgcttcttcc atgtagtgcg tcgtgtaaat gagcgacacc ccctcggcac gcagcgcttc
    14101 gatcatctga tagatgcgct cacgcgattg cggatcgacg ccaaccgttg gttcatcgag
    14161 aagtaggatg cgtggccggt gcagcgcgcc cgcagccatg ttgagacgcc gcttcatgcc
    14221 gccggaaagc gtctttaccg gttcgttggc gcgatcggtc aacgccgccc actcgagcga
    14281 tcgctccatc gcctcggcca gcgcgctacc gcggagcccg aggtagcgac cgaagacgga
    14341 gaggttctcg cgtgacgtga gattcggata gagcgcgatc tcctgcggga cgacgccggc
    14401 ctcggcgcgc gcatcggtgt cgcctgctgc cgcaggcttg ccgaagatcg acaccgtgcc
    14461 gctgtcgggg cggacgcggc ccatgatcgt gcggacgaga gtcgatttgc cggcgccgtt
    14521 agggccgagg agaccaagaa cttcggcatc gcgaagctcg agcgaaacac cgtcgagggc
    14581 ccggcgggat gcgaacgtct tcacgacgtt ctcgatttgt agtggatcca tgccgggcaa
    14641 tctactcggg gactacgggt ttgtcccctg tcgaaagtca tgagtgctgc atgacaaatg
    14701 taaggataat cggcacatgc gaaccgcaat cgtcatcgct cttctggctt cctccgccct
    14761 tgcgcagccg tcgttcgcgc cgccgccggc gatcagcgca gctcccgtcg atacgtccat
    14821 tcacagcacg ctgctgttgc cgaaagacac cacgcatgcg atgccagtcg tgttgttgat
    14881 cgcgggctcg gggccaactg atcgcgacgg caactcgccg atgctgcccg gcaagaacaa
    14941 ctcgctgcgg atgctggccg aagggctggc gatgaacggc atcgcctcac ttcgctacga
    15001 caagcgaggc atcggcgaga gcgcgaaggc gatgacgtcc gaggctgacc tccgcttcga
    15061 gacctacatc gatgacgcgg cagcgttctg cgaacaactg cgggtggaca agcgcttctc
    15121 gtcagtcgtc atcgccggtc acagcgaagg ctcgctgatc gggatggtcg cggcaaagaa
    15181 gtgcagcgcc tccggtttca tctccatcgc cgggatcggc cggcccgcag cggagatcat
    15241 ccgcattcag ctcgcaggga aactccctcc ggcattggcc acgcaaagcg acgcgattct
    15301 caaggatctc gaagaaggaa agaccacgga gaaaccgccg gtggagctcc tcgcgcttta
    15361 caggccgagc gtgcagccct acctgatttc gtggtttcgc tacgaccccg ctaaatcgat
    15421 cgcggctctg agcatgccgg tgatgatcat tcagggaacc accgacatcc aggtcagcgt
    15481 cgacgacgcg aagcgcctcg ccgccgcgaa ccctcgcgcg aagctcctca tcgtcgaagg
    15541 gatgaaccac gtcctcaaga gtgtttcgtc cgacaaggcg aagcaggcgg cgtcatacag
    15601 cgatccagcg ctgatgctcg cgcctgacct gctgatgaac attgtcgatt ttgtgcgaaa
    15661 ggcggggaag taggagaatt gggggatggc acgacccgag accgaagaag cttgggcccg
    15721 atactttgcg catcagctta gcgatagcga aagggccgag tcgcgcactc gcatgctggc
    15781 ggacttgcag caggcgaaag aagagggcgt ttttcgcgac ctccagaagt tgcgaggaaa
    15841 gattaaatgg tccatctcgc tcgacgagct tcgtgaggat cgcgattgat cgtgatcggc
    15901 ctgttcatcg aagaaatgag ccacgtactc gatagcgttc gcccgacaag gaatttggaa
    15961 tatgagtgag ctcaaaacag aagaacctgc gacaccggtc tcggatgatg gtgaggatca
    16021 ctgggcgcat taccgatcca ttcaactcac gccggagcag aaggcggctg gacgtgctcg
    16081 catgcagaag gagatcgaca aggctcgcgc cgcaggtgtc tatgaaggac tcttgaagtg
    16141 gcagggaaag gttcactggg atatcgatat cgacgaattg cgtgaggatc gcgaatttga
    16201 tcgcgattga cacgagttcc ctgcggcgtt ggtcccgaga cgagccggga atcgatgtcg
    16261 ttcgaacaag acaagtcatc ctttctggcg aagcgattct gccgccgatc gtgctcgctg
    16321 aggccctcag cagtccggct gttgaacaag aattcatcga cttcgtactg acgctccctc
    16381 tcctcgatat cgaagacggc tactggcggc gcgctggaga tctgagaagg agtttcttgc
    16441 gcgagggcta caaagccaaa ctgtcagatg ccctgatcgc gcaagcctgc atcgacaagg
    16501 acatagccct catcacccac gacgccgact tccgccactt cctccgcgcc ggactcaagc
    16561 tcgcgtagtt tccgcccgct tctgcatcca caccatcacg ccgtacagca cggccacgac
    16621 gactgcgaag ccgatgatcc acatcgactt cgatgacaga gcattgcgaa gcgcgtcggg
    16681 atctttcatc agtgacggtt gatcgccgag tacgcgcatg ccgaaccagg ccagcgcgcc
    16741 gttgaaaagg ccggcgccga ggatggtcat cagcgagaag cgggcgaagg gcatccggaa
    16801 aaggccagcc ggaatggaga tgagatgccg gattccgggc agcagccggg ccacgaagat
    16861 tccgccggat ccgtacgcct cgaaccatcg atcagctgca tcgattcttg atggcgacat
    16921 gaacacaaat cgtccatagc gtgtcaccag aggccgcccg agtttgtacg cgatccagta
    16981 gcttgcggcc gatccggccc acgagccggc cgtcgcagcg atgatgacga gcgcgatgtt
    17041 gaaccgtccc tgcgacgccc aatacgcggc gggagggatg acgacctcgg ccgggatcgg
    17101 cagcgcggtc gactcgaacg ccatcatgat gaagatgccg aggtaacccc agtcgaggac
    17161 gaactgcatc cagatggaga agaagtgatg gaaccattgc atgggcgcga atgctaaccg
    17221 aaaggccggt gttacgatcg gctcatgcag ccgcatgact atgacgtcat catcctcggc
    17281 ggggctttca gcggcgcctc gaccgcgatc ctgctgcgtc gcgaccatcc gcaactgcgc
    17341 gttctcatcg tggagaaggc cgaggcattc gacgcgaagg tcggcgaagc gacgactgaa
    17401 atgtcggcca tgtttctcac gcgccggctg gccatgtggc atcacctcga gtcggagcaa
    17461 ctgccgaaag aagggctgcg ctactggttc acgaacgaca aggtgaccgg ccacggcaac
    17521 gcgtcggaag ctggcggata tctgcgtagc gccgtgcctg cgtttcagct tcgccgcgat
    17581 gcgctcgatg agtacctgct cgcaaccgcc gtgcgcgaag gcgccgagct gttgcgcccg
    17641 gccaaagtcg tcgacgtcga tctccaggat ttcaacaaca cgatcacagt catgctgcgc
    17701 gaagatgccg aaagaccgcc cgcttctgtg gcgccgtggg aagctgcgga ttcttcggag
    17761 gcaagctcga agagcaacgg agcggaacca tcacgccgca cgttgacctg tcgctggctt
    17821 ctcgacgcta ccggacgcgt gaactttctc ggacgccgcc tcggcctgat cgagcgcaac
    17881 gacgagcatc cgatcgccgc gatctggtgc cgctggaacg gccttcgcca tatcgacgac
    17941 atcgccgcgc gcgacggcat gctggccacg cgcaacatca gttcgcgccg tctggccacg
    18001 aatcactaca ccggcttcgg ttactggatc tgggtcattc ccctcggcaa tggcgagact
    18061 tccatcggcg tcgtcttcga caagcggctg atcgaccttc atcacagtaa agatcgcgcg
    18121 accgatttca tcgccttcct gaaaggcatc ccgtcgcttg ctgaattgct cgtcgaagct
    18181 accccgcgcc tcgaagacct ccgcttctat tcgcatctac cgtacttctc gaagcagtac
    18241 atggggaacg gttgggcact cctcggcgat gcggcgtcat tcctcgatcc gtattattcg
    18301 cccggactgg atcatgcgtc ctttactgcc gaagcgacgg cggagatcgt cggtctccac
    18361 gcgaaaggcg aggacatcgc cgcgcggatc accgagcaca atgacgcgtt cacgcgctcg
    18421 tacacccgat tcttccgatc gatcttcaaa gacaagtact actacatggg agaggtcgac
    18481 cttctcggcg ctgcgatgat tctcgacacc gcccactact acatcttcgt cgtcattccc
    18541 gcgtatcgct tcgcgaagaa gtttcaatgg atgccggttc tcgggccgaa gccggcgttt
    18601 ttcagctacc acttcatcaa gttctaccat cgccgtttca aggcgatcgc gctggcgcgg
    18661 cgcgccgccg gtgaagaggg tgtgcgcaac aacggccgcc gcatcaaggc ctacttcgat
    18721 ctcaatctct ctcctttcca catgtttctg cgcggcgtga aattgtgggc gttcgccgaa
    18781 gcggacaacg tacggctgca agtacggcgg ctctttgggc gacgcgcggc gacgacggca
    18841 tcgcgggccg agggcgcggc agcacaggga tgagccgccg cgtcgcgatc gtgtttgacc
    18901 gggagttcgg tgaccgcctc ggcgacttgg cgatgcggat gcccgtctgg atcgtcgaga
    18961 gcaacacaaa ccgccccgcg gtcgccgccg cctggaaccg cgccagtgaa tggcctcaca
    19021 tcagcgtcac cgtgtttcgt ccacccgatg atttgcgcct gctcctatcg cagatcggcc
    19081 acccgcaacg tgtcgacgtg attgggttgc cactcaccga cgaagcgcgc gacactctga
    19141 tcgccgccgg cttcacaacg attgccgaga cgagcgacgg ttttcgggcg aagtgacgtc
    19201 gcctacaact ctgccagcgc cgcgcgcttg accttcccac gctcgtcgag cggaatcgac
    19261 tcgatcagtt tgatgatgcg cggcactttg tgcggcgaaa gatgatcgcg gcaccaagtg
    19321 cggatctgct cgcgcgtcac atcgggactt gcgaccaccg ctgcggcgac gacttcgccc
    19381 cgcgcacccg ccggttcacc atagactttc gcctggcgga cgccgtcgat ctgcagcaat
    19441 acctgctcga cctcgcgcgg gttcactttc ttgcttgccg tgttgatgag gtcgctggcg
    19501 cggccggtga tcgcgatctc gccgccgtcg cacagctcga tcagatcgtc ggccaggaag
    19561 cgttcgttct cgaatcgttc gaaagtgttc gcatcgtgca ggtagcccag cgagacgttg
    19621 gatccgcgca ccgtgagccg gccgcgcgcg gacgagagct gcacgttctc catcgcctgg
    19681 ccgactgtgc cgcgctcgac cgacgcacct tcccggtcgt aggtgatgcc gccgcattcg
    19741 ctgcagccgt aaaacgtgtg aatcggagtc ccgaaccgtc cgcgaaactt gcgcgatacc
    19801 acggcgggca acggcgcgcc ggcggaaaga aatgtccgaa ccgtcgagaa ttcacctgca
    19861 gccgcggtga ccagatgatc gaagaccatc ggaatcaatg gcgccaccgt gcacgcgaac
    19921 tggttgcaga gattcatgac tgactgcggc aagtagtcgt ttgagatgac gaccggtgtg
    19981 ccctgcacca acagcggcgt gaccagattc gagaatccgt acgagtgcga catcggaatt
    20041 gcgccgaggt tgatgtcgcc ctcgcggatg ttcatcgtca cgcagatgtt gaggcagtcg
    20101 gcaagcaggt ttgcatccga cgcaacgatc cccttcggca gtccggtcga tcccgaggtc
    20161 agcttgatga ggcgcgcgtt ttcaggaatg gcagcgcgtt gcgcggttcg cgtggagatc
    20221 agcacttcgt cgccgttgcg atagacgaga ccgcgggcgg agaagtgtcc gaggattctg
    20281 gccacttcgg tttccgtggc gtcgcggtcg atcaggatcg ccacgaggtt ctgcttcagg
    20341 atcgctccga atccagcaac caggtcgaca gaattcggca gctgaatcgc gacaacgtca
    20401 cgttcccgga atcccgcgcc cgcgaagcgt gccccaagct catcgatgcg ctccgcgagt
    20461 tgtgcgcgtg tggtgatccg gcccgtcgca ccgtcgatca gggcgacggt ctcaggcgct
    20521 gcaaccgcaa gagcgtagaa acgttcgagc atgtgcgtgg gggagggagg atagagtcgc
    20581 cctcgcggaa cgtcaaccgc cggtgcgctc aaatcctgag ggttgctggc aatcggcagg
    20641 atcttcatgt cccagcaatg tgtgagaatg tcgccagccc atgaacgacc taaaacgtaa
    20701 aatcaaggag ctgctgatcg agcggctcaa attcgaagat atgaccccgg acgacatcaa
    20761 agacgacgaa ccgctcttcg caggcggcct cgggctcgat tcgatcgacg cgctcgagat
    20821 cgtggtgatg ctcgaaagcg agttcggcat caaggtgaag aacgaatccg ctgcccggga
    20881 caactttcag tcggtcgccg ccctagccca attcgtcgag caacggctgg cggagcagca
    20941 ggcgcaggcg tgagctggct ggccagcctt cctcatcaga ttccgtttcg cgctgcctcc
    21001 gcggcgacgc gcaccggcga tcagacgatc gagggcgttt tcgtctgcac ggtcaacgat
    21061 gcgatgccgg cgggtgtgat ggcggtcgag gcgatggcgc agttgggagg cggcttggcg
    21121 tttgctgatt cgagctcgca cgcgtttctc accggcatcg acggctgtga agtcgatcgt
    21181 ttcatcgaga cgggtgatgt gatccgtttc gtcgttacgc ttgacgcatc tttcggcggc
    21241 acgttccgat tttcaggccg cggtctcgtc gatggagtgg aagtgatacg cagcaggttt
    21301 tatctcgcga gccaggatgc acaaagctga cacctcaacc cgcctcgcag tgaccggcat
    21361 cggcatcatc tgttcgatcg gccgcaatca ggtcgaagtg tggcgatcga tcaccgaatc
    21421 gcgctccgga atcggcgggc tgacgaagtt tccgggcgac acgtttccga cggatatcgc
    21481 agccgaggtt gatgacgatc tctatgacgc gctggagatc gatggccgcg aagcaaagcg
    21541 gatgtcgcgc agtgatctgc tcgccgtcat cgccgccagc gaagcaatgg cgcagtccga
    21601 ccgcggggcg cgcgcgccgc tgcctcgcga tcgtgccgtc gtctctaccg gaacatcgac
    21661 cggcggcctg ctcgaagggg aggactttta cttcgaccgg ctcgtacacg gacgccggcg
    21721 cgtggcgacg tcgcgcgtct tgcagcagcc gacgtccggg ccatctgacg cagtcgcacg
    21781 cgcgttgaat cttggcggcg gcgtgctgtc gaatgccacc gcgtgtgctt ccgccggtgc
    21841 cgcgatcggc gtagcggcgg atctgctgag gtcgcggcat gtggatgtcg ccgtcgctgg
    21901 aggaagtgac gcactctgcc ggctcaccta cagcggtttc aatgtcctgc aggctgtcga
    21961 tgagaagccg tgcaagccgt tcgcagcgga gcgcaagggg atcacgctcg gcgaaggggc
    22021 cgcatatctc gtgatcgagc gatgggatga cgccgtggcg cgcggtgcgt cgattctcgc
    22081 ggagctttgc ggttatggcg caacgtgcga tgcccatcat ccgaccgcgc cgcatgaaga
    22141 gggtcgtggc gccgaggcgg cgatgcgcgg cgcaatcgcg gaggcgggcg acgatccccg
    22201 catcgactac atcaacgcgc acggcacggg cacgctgctc aatgattcgg cggagacgaa
    22261 agccatcatt gccgcggtag gcgagacggt gcccgtctcg tcgagcaagt cgtacttcgg
    22321 tcatacgctg ggagccgctg gcggtatcga ggccgtgatc acagttctca ctctgcagca
    22381 ccagctcgcg ccgccgacgc tgcgtctcga catgcccgcc gcggattgca cgctcgacta
    22441 catcccgcac acgccgcggc cgatgccgat gacgaatgta ctgtccaata cattcggctt
    22501 cggtggctcg aacgtctcac tgctttttcg cagggcgtag tgctataacg cctcgccgca
    22561 tgaatcgcgt tgccatcacc ggcatcggga tcgttgcgcc ggaggcggtt ggtatcgagc
    22621 ccttccgcgc aatgctcgat gcgggaacga ctgccgtcca accgatcgac cgattcgaca
    22681 cggaaggcct ggctgcgcat accgcggcgc tcgtgcgcga tttcaaggcg cgtgactaca
    22741 tcccggcgat gaagatgcgg cggatgaata cgttgtcgcg ctacggtgtg gctgccgcgc
    22801 gcatggcgat cgacgacagt gcggcgaagc ctgccagcgg atcgggtgtc gcgatcggta
    22861 ccgcattcgg gccggtgcag acgtctgtcg attacatgca ggagtatgtg tcccgaggac
    22921 cggcgctggc gccgccgcaa ctcttcgccg agtcggttgc caacgcgccc ggatcacaca
    22981 tcgccatcga attcgacctg cgcggtttca acatcacgat gacgcagcgg gagtcgtcgc
    23041 tgctcg
//
LOCUS       DATLVW010000898         3776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_491129, whole genome shotgun sequence.
ACCESSION   DATLVW010000898 DATLVW010000000
VERSION     DATLVW010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491129"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1307
                     /locus_tag="VNN25_26665"
     CDS             159..1307
                     /locus_tag="VNN25_26665"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXH95186.1"
                     /translation="MYPHIRRSTISTAQTQQKKDWVNIAFLSLTPIIGIVGTVAWTWS
                     HGFHFWMLALLLSMYAAVGFSICAGYHRFFSHKTYEASPIVQSFFAFFGAMAAENSIL
                     WWSSAHRVHHKHVDKDWDPYNIQRGFWWAHIFWIFVKNEDFDRTFANAPDLLKNPIVM
                     WQHRWHKVILIVAGFGIPTLIGAAFGDPIAGLLWGGFLRIVVIHHTTFFVNSLAHMIG
                     KPLFNAEVSARDNWMVALLTLGEGYHSFHHRFPTDFRNGIRWYQWDPSKWLITAFRSL
                     GLASDLRTAPMPQIEQARMQAALRTVKTQIETTSGSLGDEIRRRAHVAMEHLETALKL
                     WRQHAEEKAEGLSKAWRETRRASRLHVKQSRRQWKWVVRTLRSMPEAA"
     gene            1473..>3776
                     /locus_tag="VNN25_26670"
     CDS             1473..>3776
                     /locus_tag="VNN25_26670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXH95187.1"
                     /translation="MNTTREQLGQYQILGPIGAGGMGEVYVARDPSLGRKVAIKLLPV
                     RLSGDRETLVRFTREARSASALNHPNIVTIYEVGMDQSSGSPYIVMEFIEGRDLRNLL
                     AEGPLPNRRTLDIAAQIADGLSAAHEQGIIHRDLKPENVMVTKDGYVKILDFGLAKMV
                     GTKEEEEDTARLDMPATNPGTILGTIGYMSPEQATGKGLDFRSDQFALGAILYELSTG
                     TAAFEAENAIDTLSAILHREIPSIARFNAQVPQAFCGIVDRLLSKNPKDRYASTRDLA
                     TDLHNIRDRFATKTRSEERPRPPKISRKRLMIAAIAGLVLLLAGTAAVWQRHAATIAA
                     AASPAAESKKYLAVMPFKNLTGEPNGQLVVEGLAETLSARLAHFTSVQLMRPTTPEAL
                     AETNPQKAGHNLGANIVLTGSMQRNGDRLRVAYEVVDLAKGTSHSGDLIEGSASDLFA
                     IQDKLTDSIAASLQLGAPAFRPTPHDTTISQTRYLEALGYLRRYDDESSVDSAIHILE
                     ELAAQSGSASVQAALGRAYLAKFQLTHDTKWATPAAAACERAIVADPQNPDVHVTLGE
                     LRRSMGQLDDAIKEYNAALAQQATNAEAILGLAETYKAKGKLPEAEAAYKKSIALTPN
                     YWSGYNKLGGFYANHGRPSDGISMFQKVVELSPDNLRGYNNLGAMYERLGRYDEAVQV
                     FSKSIAKRPTDQAYSNLGTCYYFLGRYADAAVAFERAAKLVPKRQVIWLNLGDAYRWV
                     PGAEAKTAAAYDEAIRLAKTDLQLNAAD"
BASE COUNT          770 a         1194 c         1126 g          686 t
ORIGIN      
        1 taatgaccgg cgatgatcgc cgatcagtgg actggtcggg aagcattatc gcctactcgc
       61 gtggtgcgtc aagtcggtta ttgccttgcg tgtcatctcg gttaaccacc ggacgctgct
      121 gcagttttgg caatacgacc ccaccgataa gggttatcgt gtatccgcac attcggaggt
      181 ccaccatcag cacagcgcag acgcagcaga aaaaagactg ggtgaatatc gcattcctct
      241 ctctcacacc gatcatcggc atcgtcggaa ccgtggcctg gacgtggtcg cacggcttcc
      301 acttctggat gctcgctctc ctcctgagca tgtatgcagc ggtcggtttc tcgatctgcg
      361 ccggctacca ccgcttcttc tcgcacaaga cgtatgaggc ctcgccgatc gtgcagtcgt
      421 tcttcgcatt tttcggagcg atggcagcgg agaactcgat cctctggtgg tcgtccgcgc
      481 atcgcgttca ccacaagcac gtcgacaagg attgggatcc gtacaacatc cagcgcggat
      541 tctggtgggc gcacatcttc tggatcttcg tgaagaacga ggatttcgac aggaccttcg
      601 cgaacgcgcc tgacctgctg aagaacccga tcgtgatgtg gcagcaccgc tggcacaagg
      661 tcatcctgat cgtggccggc ttcggcatcc ccacgctgat cggcgcagcg ttcggggatc
      721 cgatcgcggg attgctctgg ggcggattcc tccgcatcgt cgtcatccat cacacgacgt
      781 tcttcgtcaa ctcgctcgcg cacatgatcg gcaagccgct cttcaacgcc gaggtttcgg
      841 cgcgcgacaa ctggatggtg gcgctcctga cgctgggcga gggttaccac agcttccatc
      901 accgtttccc caccgacttc cgcaacggga tccgctggta ccagtgggat ccttcgaagt
      961 ggctgattac agccttccgc tcgctcggtc tcgcctcgga ccttcgcacg gcgccgatgc
     1021 cgcagatcga acaggctcgc atgcaggctg cgcttcgcac ggtcaagacg cagatcgaga
     1081 cgacctccgg ctcgctcggc gatgagatcc gccgccgcgc gcacgtggcg atggagcatc
     1141 tcgagacggc gctgaaactg tggcgccagc acgccgagga aaaagccgaa gggctttcga
     1201 aagcgtggcg cgagacacgc cgcgcgtcgc gtcttcacgt gaagcagtcg cgtcgccagt
     1261 ggaagtgggt cgtgcgcacg ctgcggagca tgccggaagc ggcgtaggaa cacgttctca
     1321 acgacgtagc gcagagccgt cttgatcgcc gtcagcccgg cggcggtcaa aagcttactt
     1381 ctgtccgtca cattacctgg tggcggatgc tctctcgaat agatgtgcct tatgtctcgg
     1441 agcgccgtag aatcgcgcta gtcccctctc cgatgaacac gacgcgcgaa caactcgggc
     1501 agtaccagat cctcggtccc atcggagccg gtgggatggg cgaggtctac gtcgcccgtg
     1561 atccctcgct cgggaggaag gtggccatca agcttctgcc cgtgcgtctc tccggcgacc
     1621 gcgagacgct ggtccgcttc acgcgcgagg cccgttccgc gtcggcgctc aatcatccga
     1681 acatcgtcac gatctacgaa gtaggcatgg accagagctc tggctcgccc tacatcgtga
     1741 tggagttcat cgaagggcgc gatctgcgca accttctcgc cgaggggccg ctgccgaatc
     1801 ggagaacgct cgacatcgcc gcgcagatcg ccgatggtct gagcgccgca cacgagcagg
     1861 gaatcatcca tcgcgatctc aagcccgaga acgtgatggt gacgaaggac ggctacgtca
     1921 agatcctcga cttcggtctg gccaaaatgg ttggaacgaa agaagaggaa gaggacacgg
     1981 cgcggctgga catgccggca acgaacccgg gcaccatcct cggcacgatc ggctacatgt
     2041 cgccggagca ggccacagga aagggcctcg attttcgttc cgatcagttc gcgctcggcg
     2101 cgatcctgta cgagctgtcg acgggaacgg cagcattcga agccgagaac gcgattgaca
     2161 ccctgtcggc gattctgcac cgcgagatcc cttcgatcgc ccgtttcaac gcgcaagtgc
     2221 cgcaagcgtt ctgcgggatc gtcgaccggc tgctttcgaa gaatccgaag gaccggtatg
     2281 cgtcgacgcg cgacctcgcc accgatctgc acaacatccg cgaccgcttc gctacgaaga
     2341 cgcggtcgga ggagaggccg cggcctccga agatttcgcg gaagcggctg atgatcgccg
     2401 cgatcgctgg tctcgtcctt ctgcttgcgg gcacggctgc ggtctggcag cgccacgcag
     2461 cgacgatagc cgcggcggcg tcacccgcag cggaatcgaa aaagtatctc gcggtaatgc
     2521 ccttcaagaa cctgacgggc gagccgaacg gacagctcgt cgtcgagggc ctggccgaga
     2581 cgctctccgc ccgtcttgcg cacttcactt cggtgcagtt gatgcggccg acgacacccg
     2641 aagcccttgc cgagaccaac ccacaaaagg ccggccacaa cctcggcgca aacatcgtgc
     2701 tgaccggctc gatgcaacgc aacggcgacc gcttgcgcgt cgcgtacgag gtcgtcgacc
     2761 tggcgaaagg aacgtcgcac tccggcgacc tgatcgaagg ctcagcctcc gacctcttcg
     2821 cgattcagga caagctcaca gacagcatcg ccgcgtcgct gcagctcggc gcacctgctt
     2881 tccgccccac gccacatgac acgacgatct cgcagacgcg ctatctcgaa gctctcggtt
     2941 acctccgccg ttacgacgac gagtcgtccg tcgacagcgc gatccacatc ctcgaagaac
     3001 tggccgcgca aagcggctcc gcgtcagtgc aagccgccct cggccgcgcc tatctggcga
     3061 agttccaact gactcacgac acgaaatggg caacgccggc agcggccgcg tgtgagcgag
     3121 cgattgttgc cgacccgcag aatcccgatg tgcacgtaac gctcggtgag ttgcgaaggt
     3181 ccatgggaca gctcgacgac gcgatcaagg aatacaacgc cgcgctggcc caacaagcaa
     3241 ccaacgctga agctattctg ggcttagccg agacctacaa agcgaaagga aagctccccg
     3301 aggccgaagc tgcttacaag aagtcaatcg ctttgacacc caattactgg agtggctaca
     3361 acaagttggg cgggttctac gcgaaccacg gacgaccgtc tgacggaatc tcgatgtttc
     3421 aaaaagtcgt ggaattgagc cctgacaatc ttcgcgggta caacaacctg ggtgccatgt
     3481 atgagcgact agggcgatac gacgaggccg tccaggtatt ctcgaaaagc atcgccaaga
     3541 gacctaccga tcaggcgtac tcgaacctgg gaacttgtta ctactttcta gggcgatatg
     3601 cggatgcggc tgtggcgttc gagcgcgcag ccaaactcgt tccgaaacgt caggttatct
     3661 ggctgaacct cggggacgca taccgatggg tgccgggcgc cgaggcgaag accgcggcag
     3721 catacgacga ggccattcgt ctcgcgaaaa cagatctgca gttgaatgcc gccgat
//
LOCUS       DATLVW010000899         3282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_408153, whole genome shotgun sequence.
ACCESSION   DATLVW010000899 DATLVW010000000
VERSION     DATLVW010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408153"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3224)
                     /locus_tag="VNN25_26675"
     CDS             complement(<1..3224)
                     /locus_tag="VNN25_26675"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXH95188.1"
                     /translation="MRLRASIILVLISAFPLFGGTFPDNAQTQKTQSITAFGSAGAVR
                     SATQDGRIIEGVSSTTGELRLIVEFRDTPLFARDRRAALATGAPALKSLQARFAQFAN
                     DLQTIHADLARKVNASSVKPTASSVVVPRITHTFAGVYAGASVIISPDALEGVSHLDY
                     VASVHLDREEHVYSTESDSKIGADQVWAQLGTQGKGIVVAVIDTGIDYNHVALGGGFG
                     PAFRVIGGHDFVNDDDDPMDDYGHGTHVAGIIGGNGGGVVGVAPQVSFLAYKVLDSSG
                     GGFDSTIMAGIERAADPNGDGNSSDHADVANMSLGRPGSPDDPVSKAVDTASAAGVVF
                     AIAAGNSGDFYTISTPANAPSAIAVGATDLDDNIAAFSSEGPVDGTFAIKPEVVAPGV
                     KIVSAKAGGGTLIASGTSMAAPHVAGVAALLRAIHHDWSVEDIKAAIVETAKVLGNDV
                     MSEGAGRIDALAAATDDLIAEPSTLNMGLDNGSSTTFSATKHVALSNRSTHAVTLSVT
                     ATGSRSGLSAAVDQPSLSLGPGETKSVSVTIVAANDQIVSPTKGSLAFSGALTVTGGS
                     VPIHVPWAMVKAATLTVTYDSANIFELFAASTSSTTLGHTISDVGNKSVDMTLPAGRY
                     DIKVSEIALQRDSNHRFFVVEDQPLENTTTVNLAPAMASNEVLLNASDDRGLPLTGIG
                     SGNGQCAQTIFLWFPSGSALTWSEVSASPNDHILLTPLSSRFTILPFERCVDFDHNAI
                     YVADFPQMRGLNGTVPATLDASAWSKQNVRILAPAGSVNPITTLVPGIIWSGANFSYM
                     DLFGTPFPLYGKAFDATLHLTPIQHPSMNGTGLFAVAVDPPPGTAVVTPNPLSHKIEA
                     VQSPAARRLGNSIVFRTYVTPKPTDYITDPAETITFGDGLLHPETSIWLDTTGQLTTT
                     YWYGAFGEIRDLEGANALLRILDGAGNVLRSARTFLPPYPPDAPGIYRLEETTPVISD
                     GISGTATLESRFDTRLEDSAPPTFTAMRLQDGNGRSVSRLQPHSAGSLLFAAIDKVPI
                     GDGGVNRALVRAEATTAQWKPHGSDTWRPLTCVIGTTDIANGQT"
BASE COUNT          642 a          982 c         1016 g          642 t
ORIGIN      
        1 tccgtctgtc cgttcgcgat atcggtggtg ccaatcacgc aggtgagtgg gcgccaggtg
       61 tcgctcccgt gaggtttcca ctgtgcggtt gtcgcttccg ctcgcaccaa agcgcggttc
      121 acccctccgt ctccaatcgg aaccttatcg atggcggcaa acaagagcga gcccgcacta
      181 tgaggctgaa gccgcgaaac ggatcgtcca ttgccgtcct gcaaccgcat cgccgtaaac
      241 gtcggcggcg cactgtcctc cagccgcgtg tcgaaccgtg actcgagggt cgcggttccg
      301 gagatgccat cgctgatgac cggcgtcgtt tcctcgaggc gatagattcc cggagcgtca
      361 ggtgggtacg gcggaaggaa cgttctggcg gaacgaagaa cgtttccggc tccgtcgagg
      421 attcgcagca gcgcgttggc gccttcaagg tctcggatct ctccaaacgc gccgtaccaa
      481 taggtcgttg tcaactggcc ggtggtgtcg agccagatgc tggtctcggg atgaagaaga
      541 ccgtcgccga atgtaatggt ctccgctgga tcggtgatgt agtccgtcgg cttcggcgtc
      601 acatacgtac ggaaaacaat gctgttgccg agacgacgcg ccgccggact ctgaacggcc
      661 tcgatcttgt ggcttagcgg gttcggcgtg acaacagcag tccccggggg tgggtccact
      721 gcgaccgcaa acaagcctgt cccgttcatg gaaggatgct ggatcggcgt aaggtgcagc
      781 gtggcatcga aggccttgcc atatagcgga aacggagtgc cgaacaaatc catataagag
      841 aagtttgccc cgctccagat gatcccgggc acgagagtcg taatggggtt caccgaaccc
      901 gcaggagcga ggatccgaac gttctgcttg ctccatgcag aggcatcgag agtagcggga
      961 acagtgccgt tgagcccgcg catctgcgga aaatcggcga catagattgc gttgtgatcg
     1021 aaatcgacac atctctcaaa cggaagaatg gtgaaccgcg agctcagtgg cgtaaggagg
     1081 atgtggtcat tcggacttgc cgacacctct gaccaggtca gagcggatcc ggacggaaac
     1141 cagagaaaga tcgtctgcgc acattgccca ttaccggaac ctattccggt gagtggaaga
     1201 ccgcggtcgt cggacgcatt gaggagaact tcgttcgagg ccatggctgg tgcgagatta
     1261 acggtggtgg tgttctcgag cggctgatct tccacgacga aaaagcggtg attcgaatcc
     1321 cgttgcagcg cgatctcgga aaccttgatg tcgtaccggc cggccggaag cgtcatgtcg
     1381 acactcttat tgcccacgtc gctgattgtg tgtcccaaag tggtcgacga agtcgatgcc
     1441 gcgaagagct cgaagatgtt ggccgaatcg tacgtaaccg taagcgtcgc ggccttcacc
     1501 atcgcccaag gcacgtggat tggcacagag ccgccggtca ctgtaagggc gcccgaaaac
     1561 gcaagcgaac ctttcgtggg tgacacgatc tggtcattcg ccgccacaat ggttaccgac
     1621 acgctcttcg tttcgcccgg gccgagcgag agtgacggct gatcaacagc cgcgctcagg
     1681 ccgctccggc tgccggtcgc agttaccgac agcgtcaccg cgtgagtcga acgattcgag
     1741 agagcgacat gtttggtcgc gctgaacgtt gtcgacgaac cgttgtcgag ccccatattc
     1801 agggtactcg gctctgcaat gaggtcgtcc gtcgccgccg caagagcatc gatacgccca
     1861 gctccttcgc tcatgacatc gttgccgagg accttggccg tctccacgat tgcagccttg
     1921 atgtcctcga cactccagtc gtgatggatc gcacggagaa gcgccgccac gccggcgacg
     1981 tgcggcgcgg ccatcgacgt gccactggca atcaacgtcc cgcctccggc cttggcggag
     2041 acgatcttca cacccggcgc caccacttcg ggcttgatcg cgaatgttcc atccaccggt
     2101 ccctcggagc tgaaagcggc gatgttgtcg tcgagatcgg tcgcgccgac cgcaattgcc
     2161 gagggtgcgt tggctggggt gctgatcgta taaaagtccc ccgagttgcc ggccgcaatg
     2221 gcgaatacca cacccgctgc cgaagccgta tcgactgctt tcgaaacagg atcatccggc
     2281 gagccgggcc ggccgagact catattggcg acgtcggcat ggtcgctcga attgccatcg
     2341 ccgttcggat ctgcggcgcg ctcgattccc gccatgatcg tggagtcgaa tccgccgccg
     2401 gacgaatcga gaaccttgta agccagaaag ctcacctggg gcgctacgcc caccacaccg
     2461 ccgccgttgc ccccgatgat tccggcgacg tgcgtgccgt ggccgtagtc gtccatcgga
     2521 tcgtcgtcgt cgttgacgaa gtcatgaccg ccgatgactc ggaatgcagg tccgaaccca
     2581 ccgccgaggg cgacgtggtt gtagtcgatt ccggtatcga tgacggccac cacgatgccc
     2641 ttgccctgcg ttccaagttg ggcccacacc tggtctgcgc cgatcttact gtcgctctcc
     2701 gtggagtaca catgctcttc ccggtcgaga tggacgctgg cgacgtagtc gaggtggctg
     2761 actccttcca gagcatcagg cgagatgatg acactcgctc cggcgtacac gcctgcaaag
     2821 gtatgtgtaa ttcgcggcac gacgacactc gaagctgtcg gtttgacgga gctcgcgttt
     2881 actttgcgcg cgagatccgc atggatcgtc tgcagatcat tggcgaattg agcaaacctg
     2941 gcttgcaagg acttgagggc tggcgcaccg gtcgcgagcg cagcacgccg gtcgcgagcg
     3001 aaaagtggcg tgtcgcggaa ctcgacgatg agccgaagct ctcccgtggt gctcgagacc
     3061 ccttcgatga ttcggccgtc ctgggtggcg cttcggaccg cacctgcgct tccgaaagcc
     3121 gtgatgctct gcgtcttctg cgtctgtgcg ttgtccggga acgtgccgcc aaaaagcggg
     3181 aacgcactga tgagcacgag aatgatcgat gcgcgaagcc tcatgagaat ctccattcgt
     3241 ccggatcccc ctgtgaagaa gcctgaccgg caatcgatcc gg
//
LOCUS       DATLVW010000900         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_122417, whole genome shotgun sequence.
ACCESSION   DATLVW010000900 DATLVW010000000
VERSION     DATLVW010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122417"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3642
                     /locus_tag="VNN25_26680"
     CDS             <1..>3642
                     /locus_tag="VNN25_26680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95189.1"
                     /translation="SDFQGSIWVVNTKGEIARYAGGNDTSGGNDSDDRLALTASIAPV
                     GIALDAANNLYLAERERNRVRKINAVTKIITTIAGNGTDGFSPDGTPAKQAKISGPTD
                     VAVDRQGNVFFSDTGNGLIRRIDAASGNISTWAGGGNPPTGNGDGGTATNAVITPKFM
                     AIDPKNDDLYVADENGLRLRKIDAKTQVITTVAGSGVDYTDREFSGDNGAAVKAKLNF
                     VFTESGVAIDAAGDLFISDTVNDRVRVVNACSTVTAPQLTQPADSANVSTAPKLAWAA
                     VDHAFRYDVLLDTVNPPVRVFAADISDVSFTPANLQPSTRYYWSVKAKGDPFCVPVST
                     AVSRVATFTTDATCAAGAFDSIAPQNGATVSSSPVQLSWQSAGDGATYDVYLGTLANP
                     PLVASAITTTSYSATIVSGQYSWFVVAHAGCDSSKTTASLTRSFTSSLSPGCSPGQLQ
                     VIASRPSDGATDISSTFDLGWVPLGVASSYDLYFGTSSQPPLLIGGLDRTSQTVTSLA
                     PGTKYFWRVVAKGPCDPNGVSSSIVSFTTRSCAAAGATSIVFAPSTVPEGATYAIVWS
                     VAPGLDSDGGYLVERSTNSSFSPLLDSQVISSTAASFFADNAGNIFHRVRALPGCDPS
                     KSGPPSAVIIVNVTPARPNVVFTLLPTALVTGLGDHLEDKRGSFALENLGTSSIQVIV
                     GRQELNGSAPFFTIVDTTATDSAFVTLEPRKPHLFEIRYAGPSNTIAASYQGVIFVAA
                     TGEGLAVTPYAFVNLKVGGGAAATPQFLIGGTPTDYVAFPPLTGDDANRSPLSVSIKN
                     NGTTTMELAAEIGPEVWLVPDATWNATGLAPGATRTLNLSTRRSRAPNGSPLPRYTYF
                     GVRTKDGQTARLLVQDSEELLLTTGRTIRLDLDTRSFIVPDAISRTNSQSLPLVTQLR
                     LSNIGSGSVQAELIFTPENADGFDTTAVKRVIVIVPANDVVTLTDPLVQIFHLTRPSR
                     GSIEVRLPPERVGLLRASASVITLGGGGGFVTPVVTRGEGARIAAPQSISGITSNASV
                     ITSLNLAETSGIDHAAVQLTLYDANGNSAGTLSVDVPAYGVKHFDNIVAAMNGPAIDP
                     GQLQIAVTAGGGSVIGVASAGSADGGATFVSTPLTSSAAKAILAFSRANDTTSSVNVK
                     TVVPVLATASGTSGSPATFKTAVAFAAPVGVPGTFFATFLDSAGGNTSPRQTITVAAS
                     ATKIYGDVI"
BASE COUNT          652 a         1307 c         1081 g          602 t
ORIGIN      
        1 tctccgattt ccagggctcg atctgggtcg tcaacaccaa aggcgagatc gcgcgatacg
       61 caggcggcaa tgatacgagc ggcggcaacg acagcgacga ccgcctcgcg ctcacagcca
      121 gcatcgcacc ggtcggcatc gccctcgacg cggccaacaa tctctacctc gccgagcggg
      181 aacgaaaccg cgtgcgcaag atcaacgcgg tcacgaagat catcaccacg atcgccggaa
      241 acggaacgga cggcttcagt ccggacggca caccggcgaa gcaagcaaag atcagcggac
      301 cgaccgacgt cgccgttgac cggcagggca acgtcttctt ctccgacacc ggcaatggcc
      361 tgatccgccg catcgacgcc gcgtcgggaa acatctccac ctgggcgggt ggcggtaacc
      421 cacccaccgg caacggcgac ggcggcaccg ccacgaacgc cgtgatcacg ccgaagttca
      481 tggccatcga tccgaagaac gacgacctct acgtcgccga cgagaacgga ttgcggctgc
      541 gcaagatcga cgcgaagacg caggtcatca ccaccgtcgc cggcagcggc gtcgattaca
      601 ccgaccgcga gttttccggc gacaacggcg cggcggtcaa ggcgaagttg aatttcgttt
      661 tcaccgagtc gggcgttgcc atcgacgccg cgggcgatct cttcatctcc gacacggtca
      721 atgaccgcgt gcgcgtcgtc aatgcctgct ccaccgtcac cgcgccgcag ttgacgcagc
      781 ctgccgactc cgcgaatgtc tcgaccgcgc cgaaactggc gtgggccgcg gtcgaccatg
      841 ccttccgcta cgacgtgctg ctcgacacgg tcaatcctcc cgtccgcgtg tttgctgccg
      901 acatcagcga cgtgtcattc acgcccgcga acctgcaacc atccacgcgc tattactgga
      961 gcgtgaaagc gaagggcgat ccgttctgtg tgccagtttc caccgccgtc tcgcgcgtcg
     1021 cgacattcac gaccgacgcg acgtgcgctg caggcgcgtt cgattcgatc gcaccgcaga
     1081 acggcgcgac cgtcagcagc tcgccggtgc agctctcatg gcagtccgcc ggcgacggtg
     1141 cgacctacga cgtctacctc ggcaccctcg ccaatccgcc gctcgtcgcc tccgccatca
     1201 cgacgacgtc gtacagcgcg acgatcgtct ccggccagta ctcgtggttc gtcgtcgcgc
     1261 acgccggctg cgattcatca aagacaacag cgagcttgac gcgctcgttc acctcgtcgc
     1321 tctcccccgg ctgttctccg ggccagttgc aggtgatcgc ctcccgcccg tccgacggcg
     1381 ccaccgacat ctcatccaca ttcgacttgg gatgggtccc gctcggcgtg gccagcagtt
     1441 acgacctcta cttcgggacg tcgtcgcaac cgccgctgct catcggcgga ctcgatcgca
     1501 ccagccagac cgtcacgtcg ctcgctcccg gcacgaagta cttctggcgc gtcgtcgcga
     1561 aaggcccgtg cgatccgaac ggcgtgtcct cgagcatcgt cagcttcacg acgcgaagct
     1621 gcgccgccgc aggcgcgacg tcaatcgttt tcgcgccgtc gacggtgccc gagggagcga
     1681 cgtacgcgat cgtgtggtcg gtggcgccgg gattggacag cgatggcggc tacctcgtcg
     1741 agcgatcgac gaattcgtca ttcagtccat tgctcgacag ccaggtcatc tcgtcgaccg
     1801 ccgcatcgtt cttcgccgat aacgcgggca acatctttca ccgcgtacgt gcgttgccgg
     1861 gatgcgatcc gtcgaagagc ggcccgcctt ccgctgtcat catcgttaac gtgacgccgg
     1921 cccggccgaa tgtggtcttc acgctgttgc cgacggcgct cgtcaccggt ctgggcgatc
     1981 acctcgagga caagcgcggc tccttcgcgc tcgaaaacct cggaacctcc tccattcaag
     2041 tgatcgtggg ccggcaggag ctgaacggct ccgccccctt cttcacgatc gtcgatacga
     2101 ccgccaccga ctccgctttc gtcacactcg agcctcgcaa accgcatctc ttcgagatcc
     2161 gctacgccgg gccgtcgaac acgatcgccg cgagctacca gggcgtgatc ttcgtcgctg
     2221 cgaccggcga agggctggca gtgactccct acgcgttcgt caatctcaaa gtcggcggcg
     2281 gcgcagcggc gacgccccag ttcctcatcg gcggcacgcc cacggactat gtcgccttcc
     2341 cgccgctcac cggcgacgac gccaatcgct ctccgttgag cgtctcgatc aagaacaacg
     2401 gcacgacgac gatggagctg gccgcggaaa tcggtcccga agtctggctc gttcccgatg
     2461 cgacctggaa cgcgaccggg ctggcgccgg gagcgacacg aactctgaat ctgtcgacgc
     2521 ggcgcagccg ggcgccgaac ggatcgccgc ttccgcgata cacgtacttc ggcgtccgca
     2581 cgaaagatgg ccagaccgcc cggctgctgg tgcaggacag cgaagagctg ctgctcacca
     2641 ccggacgcac catccgcctc gatctggaca cgcgatcgtt catcgtgccg gacgccatca
     2701 gccggaccaa ttcgcagtcc cttccgctgg tcacgcaact gcggctcagc aacatcggca
     2761 gcggctccgt gcaggccgag ttgatcttca ctcccgaaaa tgcagatggc ttcgacacca
     2821 ccgccgtcaa gcgcgtcatc gtgatcgtgc ccgccaacga cgtcgtgacg ctgaccgatc
     2881 cactcgtgca gattttccat ctgacgcggc catcgcgcgg ttcgatcgag gtgcgcctgc
     2941 cgcccgagcg cgtcggactg cttcgcgcga gcgcgtccgt gatcacgctc ggcggcggtg
     3001 gcgggttcgt tacgccggtg gtgacacgcg gtgaaggcgc acgcatcgcc gcacctcaaa
     3061 gcatcagcgg catcacatcg aacgcgtcgg tgatcacgtc gctgaacctc gcggagacga
     3121 gcggcatcga tcatgccgcc gttcaactga cgctgtacga cgcgaacggc aactccgccg
     3181 gaacgttgag cgtcgatgtc cccgcctacg gcgtgaagca tttcgacaac atcgtggccg
     3241 cgatgaacgg accggccatc gatcccggcc agttgcagat cgcggtcacg gccggcggcg
     3301 gatcggtcat cggcgttgcc agcgccggca gcgcggacgg cggcgccacg ttcgtcagca
     3361 cgccgctgac cagcagcgcg gccaaggcca tcctcgcctt ctcacgagcg aacgacacca
     3421 cgtcctccgt caacgtcaag actgtcgttc cggtgctggc cactgcatcc ggcacgagcg
     3481 gttcacccgc cacgttcaag accgcggtcg cattcgccgc gccggtcggc gtgccgggga
     3541 cgttcttcgc caccttcctc gactccgccg gaggcaacac atcgccccgg caaacgatca
     3601 cagtcgcggc ctcggccacc aagatctacg gcgatgtgat ca
//
LOCUS       DATLVW010000901         6685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_408205, whole genome shotgun sequence.
ACCESSION   DATLVW010000901 DATLVW010000000
VERSION     DATLVW010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408205"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VNN25_26685"
     CDS             complement(<1..597)
                     /locus_tag="VNN25_26685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95190.1"
                     /translation="MNRTRTILVAALFALFATGCFEVEQSINLNRDMSGTANFKLGID
                     MEPMVIIMAKVQKEMGGDKSPLTKAEIAAAKADFKKSEKKSTETPADPRKQTEGGLPP
                     GVKLLDASVTEKEFGVTTNMKFAFDKLSNLVGVKLGTKKDAGGPADPTKSSVIDSPFQ
                     GLEISETAKTISIHTKPQNPAEKVQSQANAEGGPKMDPE"
     gene            complement(690..854)
                     /locus_tag="VNN25_26690"
     CDS             complement(690..854)
                     /locus_tag="VNN25_26690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95191.1"
                     /translation="MNTTTQPDERDSMMDERVNTPDEPDRTAGKHDRTPVKRSSTPNE
                     CASTAVRRDG"
     gene            complement(1130..1699)
                     /locus_tag="VNN25_26695"
     CDS             complement(1130..1699)
                     /locus_tag="VNN25_26695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95192.1"
                     /translation="MVKYNVISDEWFYLLLPKGQPFDQFEAADGTQDREKTKTVLSQM
                     AGELPRSFSEILHLSHFEYSVTLLGRVDEALTPDVIESWMEKSDIESWMEKSDPDDPN
                     NFFKLTLSELAVYLGSLLIRHHGGEWHYSRFPNFFQSSVVINDIAFHVFDTLMKRCSE
                     DHGHEKLLSKWSIFNDVLASSAVTPDKVS"
     gene            complement(2049..3047)
                     /locus_tag="VNN25_26700"
     CDS             complement(2049..3047)
                     /locus_tag="VNN25_26700"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXH95193.1"
                     /translation="MKSSKKNLAERWTVESLRPIVQRLTTGGTIGPGASDIPLIDQEV
                     DLRGIHLDLNSAPGVHVITDMRALHDQPVRRIVGARFQSVNFSFASLPNLWVESCEFT
                     NVNFANADLSGWADHGNVFRNCTFEGASLESASLGHRGSTYTSCTFSRTKLRSTTFAR
                     PEFKGCAFADRRISDLDFFASSFERCAFSGRLEDLWFRGGYPAPAEAAEREFGKARPN
                     RMLHVDFSAAELIGTTFSDGCDLSTCVLPRDDQVSRYSGWQSTVSIAEELVSDFFSGD
                     DAVEAKTLLRAYRSDSRQEWYIINFRDVAAAVHDDEEFADRFRSLLTKANEAAAVA"
     gene            complement(3054..>6685)
                     /locus_tag="VNN25_26705"
     CDS             complement(3054..>6685)
                     /locus_tag="VNN25_26705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HXH95194.1"
                     /translation="SSDLPTQTSNGERGGSYSQTFGYNSAGETIRATLAGLSSDQHFD
                     QGGNVTSRTEPNRNEAKYEYDSRGALTKETLPDLKQIAHETSALGVSTKYTDQVAQDT
                     AVTDKDFIGRPLLRKYPDNTTEHFEWEGPRISTYTNRQGQKVTYNYYPDSGKLFQIVG
                     SVILDEFHYDAAGRISVMKTPDAEIDIDSYDNEGHPLHVKEMRIVDGVPSPSSTLEQT
                     YTWNAHGERTSWTMPRLGTPQNGWTDTVFEDHDEAGNVIGISRTQFGSGTSVPAALLG
                     AVFRNAGRPDQRTVTTSCAGVQVCSGGSILRKYGYNANAQMNSLTVTSSGGALAGSVA
                     NYEANGLQIHDVQILGLAGGAYYNVYGYDDRSRLQGAVLNTPDPNSPPGGSVPGTLGM
                     NYTNPDNPDGPADFLGGVTRVPRLDSATRSGLTQQGVDVDKIDPPSTTATPTSGGHKI
                     AKFGPVGGTPRTFGYAGSLVSDNGKYVYNWNEKEQLVAVTQKPTATAFPIRRIRYYYD
                     GRGRVVGRRAQTANVATLSDPLDSLQWQTETNPSLLAADGLPADATFAWDMVSDQLIA
                     VFNTNPPQGDPNAGLMRQIIHGGLTYDDPIEVTMLAADVSGKLNRLYPVFDEAGAGSL
                     QAVLNVDAQLVARNLPDDSDGSEDIDFAGATIDRASIHATKDSSGNLQSIDVTLHATE
                     PLEAATLASGLRLASVTTTGATVKTFAGAAQAVSGDAYSAKFTLSATDWSTLNDPAAQ
                     ALSIGVTPTLRAHGWAGGLPILAPTEWAKLNGIFTSPALPVELRDSLSSISTFITDIG
                     NNSEKTTTIYNVPSVALVGSTGTGADATSAIFTAKFQALPFSDPATGLVYARARWYDP
                     ETGAFLSGDPMAYEDSSNLYSFAGGDPVNRRDPTGDCWDLTDPKCRQEWADTGKEFVR
                     LTFTDPKAADRNAEHIIGGVEGVANFGKKTVTGLVSLAQDTSVVGLMTDPIGATQRNI
                     DRAKAIANFASHPIETIKKTHAESANRILDAEQRGDYEAAGAEAGEIGSADAAAIIGA
                     GEGAVSLAKLGTRLLGSAAATETVIAEAAETTVAVPEGGAPFETGAPVGFDSTESYLA
                     ELREMQHAEVTANMQAHVTRAAQAVDAQGLNAFTLRQRFAMLRNPNLSAAFRGQRIDR
                     LARTSIMNDPVIRGQLPTLRGSINLGPDFVSMSSGLWWDMTTLRSWAAHVAKYGAGGT
                     HLITR"
BASE COUNT         1204 a         1970 c         2114 g         1397 t
ORIGIN      
        1 ttcgggatcc attttcgggc cgccctccgc gttggcctgg gactgcactt tttccgcggg
       61 gttctgcggc ttcgtgtgaa tggagatcgt cttcgcggtc tcggagatct ccagtccctg
      121 gaacggcgaa tcgatgacgg agctcttcgt cggatcggcc ggccctcccg catctttctt
      181 cgtgccgagt ttcacaccga cgaggttgga gagcttgtcg aatgcgaact tcatgttggt
      241 ggtgacgccg aactcctttt cggtgaccga ggcgtcgaga agcttgacgc cgggcggaag
      301 gccgccctcc gtctgtttgc ggggatccgc gggtgtctcc gtgctcttct tctcgctctt
      361 cttgaagtcc gccttcgccg ccgcgatctc cgccttcgtc agcggactct tgtcgccgcc
      421 catctctttc tgaaccttgg ccatgatgat gaccatcggc tccatgtcga tgcccagctt
      481 gaaattcgcg gtaccggaca tgtcccgatt cagattgatc gattgctcga cttcaaaaca
      541 gccggtagcg aagagggcga agagcgccgc aacaaggatc gtgcgtgtgc gattcattcg
      601 gattctcctt ttcgggaaag tatagatcgg gaccagtcct gtcaggagaa tccgccggac
      661 tgtcgcggca aaccgccgga cgatcagggt tatccgtcgc ggcgaaccgc cgtgctggcg
      721 cattcgttcg gcgtgctgct gcgttttacg ggcgtgcggt cgtgcttgcc ggccgtgcgg
      781 tcgggttcat cgggcgtgtt gacgcgttca tccatcatgc tgtcgcgctc atctggctgt
      841 gtcgtcgtgt tcatcacgct cgatgcaagc cgtcgtcagt ttcggtttcg ccctgacccc
      901 acattgaagg cccaggacgc cagcctgctg gctctaggga aacgcaaggc cggcgtcgca
      961 ataaaggaag ttgcagcatg atcgtcacct gatgccgcag acttccttcg agcggcccgg
     1021 cagaggcgct cgacgcgctt catgaccatc gttctcgatc ttcgaatggg gggcacaaat
     1081 caaaaagagg tcgcgctcca aacggtctct gacgttggaa ccggccgctt caactcactt
     1141 tgtcaggggt taccgcggac gacgcaagca cgtcattgaa tatgctccac ttcgagagca
     1201 gcttctcgtg accgtgatct tcgctacacc tcttcatcaa cgtatcgaag acatgaaacg
     1261 cgatgtcgtt gatgaccaca ctcgactgaa agaaattcgg gaatcgtgag taatgccact
     1321 cgccgccatg atgacgaatg agaaggctgc ccagatagac agccaattcg gacagagtga
     1381 gcttgaaaaa gttgttggga tcatccggat cagacttttc catccatgac tcaatatcag
     1441 acttttccat ccatgactca attacgtcag gtgtcaaagc ctcgtcaacc cgtcccaaga
     1501 gtgtgaccga atactcaaaa tgggatagat gcaatatttc ggagaacgag cggggcaact
     1561 ccccggccat ctgagacagg actgtcttcg ttttttcacg gtcctgcgta ccatctgcag
     1621 cctcgaactg atcgaagggc tggcccttcg gcaagagcaa ataaaaccat tcatcagaaa
     1681 tcacgttgta cttgaccatt tacgggctcg ggtgcgtcgg cgtatggatg accggcaggc
     1741 tttggataat ggtacggccg gtcgagtaat caaacacttc gacaatgatg ccgagcctgg
     1801 tcgtctcagc aacaaactgt ctgaagactt gatcttacgg aaaagtcatc ttggggccaa
     1861 cgaagcgggg tcaggtcgaa accgaaactg gtgacaactc gctacaagca ttaggctcga
     1921 ggcacgccgt tttagtttcg gcctcaagct gtcccacatg gaaaagaact gccggatctc
     1981 cgattgcggg cgccgtgagc atcgccggcc tcgtcccaag tcatactcat gggtggtgcc
     2041 gcaggctgtt atgcgactgc ggccgcttcg ttagcctttg ttagcagaga tcggaacctg
     2101 tccgcgaatt cttcgtcgtc atggacggcg gcagcgacgt cgcgaaagtt gatgatgtac
     2161 cactcctggc gcgaatcgga acgataggcc cgcaacaagg ttttcgcttc taccgcgtcg
     2221 tcgccactaa agaagtcact tacgagttct tcagcgatcg agaccgtcga ctgccacccc
     2281 gaatatcgtg agacctgatc gtcacgcgga agtacgcacg tgctcagatc gcacccgtcg
     2341 ctgaacgtag ttccgatcag ctcggcagcg gagaaatcga cgtgcagcat tcgattgggc
     2401 cgagcttttc caaactctcg ctcggcggcc tcggccggag cggggtagcc gccgcgaaac
     2461 cagagatctt ccagccggcc gctgaacgcg cagcgttcga atgacgaggc aaagaaatcg
     2521 aggtcgctga ttctgcgatc ggcgaatgcg caccctttga attccgggcg cgcgaacgtg
     2581 gtagagcgaa gcttcgttcg cgaaaacgtg caagacgtgt atgtactacc gcgatgccct
     2641 agcgatgcgc tttcgagcga tgccccctcg aacgtgcagt tgcggaaaac gttcccatga
     2701 tcggcccagc cgctcagatc ggcgttcgcg aaatttacgt tcgtgaattc gcatgactcg
     2761 acccagagat tcggtagaga tgcgaagctg aagttgaccg actggaagcg agctccgacg
     2821 attcgtcgaa cgggttgatc atgcagagcg cgcatgtcgg tgatgacatg aactccgggt
     2881 gcgctattca gatcgaggtg aatgccccga agatcgactt cctgatcgat cagggggatg
     2941 tcggatgcgc cgggaccgat cgtgccgccg gtagtcaaac gctggacgat cggccgtagt
     3001 gactcgaccg tccagcgctc cgccaggttt ttcttgctgc tcttcattaa acctcaacga
     3061 gtgatcaagt gagtcccacc agctccatac ttcgcgacgt gcgcggccca cgaccgcaag
     3121 gtcgtcatat cccaccacag gcccgatgac atgctcacaa aatcaggacc aaggttgatc
     3181 gagcccctca gcgtcggtaa ctggccgcga atcacaggat cattcatgat cgaagttcga
     3241 gcaagcctgt cgatgcgttg accacggaat gctgcactta ggttcggatt cctgagcatt
     3301 gcaaaacgtt gtcgcaatgt aaatgcgttc aggccttgtg cgtcaaccgc ctgggcagct
     3361 cgcgtgacgt gcgcctgcat gttggctgtg acttccgcat gctgcatctc gcgaagctcg
     3421 gcgaggtagg actcggtgct gtcaaatccg acaggcgcgc cggtctcgaa cggtgcccct
     3481 ccctccggca ccgcgaccgt tgtttcagcg gcctccgcga tgactgtttc ggtggccgcg
     3541 gctgaaccga gcaggcgggt tcccaacttc gccagcgata cggcgccttc tcccgccccg
     3601 atgattgccg ccgcgtccgc cgagccgatc tcgccggcct cggcgccggc tgcttcgtag
     3661 tcacctcttt gctcggcatc gagaatccga ttcgctgact ccgcgtgcgt tttcttgatc
     3721 gtttcgatcg ggtggctggc gaagttcgcg atggccttcg cccgatcgat gtttcgctgg
     3781 gtggcaccga tcggatcggt catcagacca acaacgctcg tgtcctgcgc cagcgatacg
     3841 aggccggtca ctgtcttctt cccgaaattc gcgacgccct caacgccgcc gatgatgtgc
     3901 tccgcattgc gatcggccgc tttcgggtcg gtaaaggtga ggcggacgaa ttctttcccg
     3961 gtgtcagccc attcctggcg gcacttcgga tcggtcagat cccagcaatc gccggtcggg
     4021 tcgcgccggt tcaccggatc gccaccggcg aaggagtaca ggttcgagga gtcctcgtac
     4081 gccattggat cccccgaaag gaacgccccg gtctcggggt cgtaccaccg cgccctggcg
     4141 tagaccaatc ccgtcgccgg gtcgctgaat ggcagcgcct ggaacttcgc ggtgaagatt
     4201 gcgctggtgg cgtcagcgcc cgtgcccgta gagccgacga gggcgacgga cggcacgttg
     4261 tagatcgtcg tcgtcttttc gctgttgttg ccgatgtcgg tgatgaacgt cgagatggaa
     4321 ctgagcgaat cgcggagctc gaccgggagg gctgggctgg tgaagatgcc gttcagtttg
     4381 gcccactcgg tgggggcgag gatggggagg ccgccggccc agccatgggc gcggagggtg
     4441 ggggtcacgc cgatggagag cgcctgggcg gcggggtcgt tgagcgtgga ccaatccgtg
     4501 gcggacaggg tgaacttcgc ggagtaagcg tcgccgctga ccgcttgcgc ggcgccggcg
     4561 aaggtcttga ccgtcgcgcc ggtcgtggtg accgacgcga ggcgcaggcc gctggcaagc
     4621 gttgctgcct cgagcggctc ggtggcgtgc agcgtcacgt cgatcgactg caggttgccg
     4681 gacgagtcct tcgtggcgtg gatcgacgcg cggtcgatcg tcgcgccggc gaagtcgata
     4741 tcctccgagc cgtcactatc gtcggggaga ttgcgcgcga cgagctgggc gtcgacgttg
     4801 agcaccgcct gcagcgagcc agcgccggct tcgtcgaaga ccggatagag gcgattcaac
     4861 ttgccgctga catcggcggc gagcatcgtc acttcgatcg gatcgtcgta ggtcaggccg
     4921 ccgtggatga tctgccgcat caagccggcg ttcggatcgc cctgcggcgg attcgtgttg
     4981 aagaccgcga tcaactgatc cgagaccatg tcccacgcga acgtcgcgtc ggccggcagg
     5041 ccgtcggcgg cgaggagcga cggattcgtc tccgtctgcc attgcagcga gtcgagagga
     5101 tcggagagtg tggccacgtt cgcggtctga gcgcggcgac ccacgacgcg gccgcggccg
     5161 tcgtagtagt agcgaatgcg gcggatcggg aacgcggtgg ccgtaggctt ctgcgtcacc
     5221 gcgaccaact gctccttttc gttccagtta tagacgtact taccgttgtc gctgacgagc
     5281 gagccagcgt agccgaacgt ccgtggggtg ccgccgaccg gaccgaactt ggcgatcttg
     5341 tggccgcccg aggtcggagt ggcggtggtg ctgggcggat cgatcttgtc gacgtccacc
     5401 ccttgttggg tgagcccgga acgcgtggcg ctatcgagcc gcggaacgcg tgtgacgccg
     5461 ccgaggaaat cggcagggcc gtcgggatta tcgggattgg tgtagttcat gccgagcgtg
     5521 cccggaaccg agccgcccgg aggggagttt ggatcgggtg tattcagaac ggcgccctgc
     5581 aagcggctgc ggtcgtcgta gccgtagacg ttgtagtacg ccccaccggc caggccgagg
     5641 atctggacat cgtgaatctg caggccgttg gcctcgtagt tcgcgaccga gccagccaac
     5701 gcaccgccgc tgctcgtgac ggtgagcgag ttcatctgcg cgttcgcgtt gtagccatac
     5761 tttcggagaa tcgagccgcc gctgcagact tggacgccag cgcaactggt cgtgactgtg
     5821 cgctgatcgg ggcgaccggc gttgcggaac accgcgccga gcagcgcggc cggcacggac
     5881 gtgccggagc cgaactgcgt ccggctgatg ccgatgacat tcccggcctc gtcgtgatct
     5941 tcgaagacgg tgtcggtcca tccgttttga ggcgtgccga ggcgaggcat cgtccaggag
     6001 gtgcgctcgc cgtgcgcatt ccaggtgtag gtctgttcga gcgttgacga cggcgacggg
     6061 acgccgtcga cgatgcgcat ttccttcacg tggagcggat gaccttcgtt gtcgtacgaa
     6121 tcgatgtcga tctcggcatc cggcgtcttc atgaccgaga tgcggccggc ggcgtcgtaa
     6181 tgaaactcgt cgaggatcac gctgccgacg atctggaaca gtttccccga atcggggtag
     6241 tagttatagg tgaccttctg gccctgacgg ttcgtatacg tcgagatacg ggggccttcc
     6301 cactcgaagt gctcggtcgt attgtccgga tacttgcgga gcagcggccg gccgatgaag
     6361 tccttgtcgg tgacggcggt gtcctgcgcg acctggtcgg tgtacttggt ggaaacgccg
     6421 agggcgctgg tctcatgggc gatctgcttc aaatcgggca gcgtctcctt cgtcagcgca
     6481 ccgcggctgt cgtactcgta cttcgcctcg ttgcggttcg gctcggtccg gctcgtgaca
     6541 ttgccgccct ggtcgaagtg ctgatccgac gaaaggccgg cgagcgtcgc gcggatcgtt
     6601 tcgccggcgc tgttgtagcc gaacgtctgc gagtacgagc cgccgcgctc gccgttgctc
     6661 gtctgcgtcg ggaggtcgct cgatc
//
LOCUS       DATLVW010000902         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_327685, whole genome shotgun sequence.
ACCESSION   DATLVW010000902 DATLVW010000000
VERSION     DATLVW010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327685"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VNN25_26710"
     CDS             complement(<1..155)
                     /locus_tag="VNN25_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HXH95195.1"
                     /translation="MARSVWKGPFVDGYLLNKADAARQSGRNEIIRIWSRRSTILPQF
                     VGLTFGV"
     gene            complement(157..987)
                     /gene="rplB"
                     /locus_tag="VNN25_26715"
     CDS             complement(157..987)
                     /gene="rplB"
                     /locus_tag="VNN25_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXH95196.1"
                     /translation="MPVKNYKPTSAGIRFQSHHTFDEITKTKPEKSLTKGKPKTGGRN
                     SGGRITSRFIGGGHKQKYREIDFRREKHGIPAKVSAIEYDPNRTARIALLNYADGEKR
                     YILAPSGLEVGSTIMSGPDADILVGNSLPLSKIPLGTTIHNIELKEGKGGQMARSAGA
                     FAQLMAREGDWATLRLPSSEMRKVHVRCYATIGQVGNLEHENVSIGKAGRTRHAGKKP
                     HNRGVSMNPVDHPLGGGEGKTSGGRHPVTPWGKPTKGYKTRSNKRTDGMIIRRRNKGK
                     "
     gene            complement(1016..1309)
                     /locus_tag="VNN25_26720"
     CDS             complement(1016..1309)
                     /locus_tag="VNN25_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXH95197.1"
                     /translation="MNANQIIRRPLVTEKSTMMRDAGDNILAFEVDVKANKIQVKSAI
                     EELFKVKVQEVRLFNVRGKIKRMGRYQGKRRDWRKAYVRLEKGQKAPNFIEGV"
     gene            complement(1306..1932)
                     /gene="rplD"
                     /locus_tag="VNN25_26725"
     CDS             complement(1306..1932)
                     /gene="rplD"
                     /locus_tag="VNN25_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXH95198.1"
                     /translation="MPTIDIKDWNNKKVGSVDLPEAIFAYPYKEHLIHEAVRNYLASL
                     RQGTHKVKNRSEVSGSGKKPFRQKGTGRARQGGARPPIHRHGGTVFGPTPRDYSYKMN
                     AKEKKNALKSALSQRVKEGKLVLLNDLAVDEAKTQALAAKFTGIGVEGKALLVDSFEN
                     ANLALAARNNPKLTLVDALHVTVYDVVNSRFVVLSQDALQRLTEALES"
     gene            complement(1934..2575)
                     /gene="rplC"
                     /locus_tag="VNN25_26730"
     CDS             complement(1934..2575)
                     /gene="rplC"
                     /locus_tag="VNN25_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXH95199.1"
                     /translation="MAIEGIIGKKVGMTQVFADDGALVPVTVIQAGPCLVVQKKTSEK
                     DGYDAVQVGLVEKISNRRITSAARGHFEKAGVPPVRTLVEFPYEGDANVGDRVQVDIF
                     KAGDSIDVVGQSKGKGFQGVMKRHHFGGGRATHGSMFHRAPGSIGASAFPSRVMKGMR
                     MGGRMGGDQVTVKNLRVAKVDAENNLLYIRGAVPGGKNGLVVVRFSRNGKRGE"
     gene            complement(2614..2928)
                     /gene="rpsJ"
                     /locus_tag="VNN25_26735"
     CDS             complement(2614..2928)
                     /gene="rpsJ"
                     /locus_tag="VNN25_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXH95200.1"
                     /translation="MNEKIRIRLKAYDYRVLDQSTSEIVDTAKRTGARVAGPIPLPTQ
                     IARYTVLRSPHVDKKSREQFEMRTHKRLLDILEPTTQTVDALMKLELPAGVDVEIKAF
                     GK"
BASE COUNT          458 a          915 c          902 g          654 t
ORIGIN      
        1 tagacgccga aggtcagccc gacgaactgc ggcaggatgg tggaccgccg cgaccagatg
       61 cggatgatct cgttgcggcc cgactggcgc gcggcgtctg ccttgttcag cagatagccg
      121 tcgacgaacg gacccttcca gacggaacgc gccatcctat ttgcccttgt tgcggcggcg
      181 gatgatcatg ccatccgtcc gcttgttgct gcgcgtcttg taacccttgg tcggcttgcc
      241 ccacggcgtc accggatgac ggccgcccga ggtcttgcct tcaccgccgc cgagcgggtg
      301 atcgacaggg ttcatcgaga cgccgcggtt atgcggtttc tttcccgcgt gacgcgtgcg
      361 gcctgcttta ccgatcgaga cgttctcgtg ctcgagattt ccgacctgac cgatcgtcgc
      421 atagcagcgc acgtgaacct tgcgcatctc gctcgaaggg agacgaagcg tcgcccagtc
      481 gccttcgcgc gccatcaatt gagcgaaagc cccggccgaa cgagccatct gtccgccctt
      541 tccctctttc agctcgatgt tgtgaatcgt cgtgccgagg gggatcttgg agagcgggag
      601 ggagttgccg acgaggatgt cggcgtcggg gccggacatg atcgtcgaac cgacttccag
      661 tccgctcggc gcgaggatgt agcgcttctc gccgtcggcg tagttcagca gggcgatgcg
      721 cgccgtgcgg ttcgggtcgt attcgatcgc agagaccttc gccgggattc cgtgcttctc
      781 gcggcggaag tcgatctcgc gatacttctg cttgtggccg ccgccgatga agcgcgaagt
      841 gatccggccg cccgagttac ggccgccagt cttcggcttt cctttcgtca gcgacttctc
      901 cggcttcgtc ttcgtgatct catcgaacgt gtgatgggac tggaagcgga tgccggcaga
      961 cgtcggtttg taattcttga caggcatagt gtcctctgtt tatcgcctta ggaagctaga
     1021 cgccctcgat gaagttcggg gccttctgtc ccttttcgag ccgcacgtag gccttgcgcc
     1081 agtcgcggcg cttgccctga taacggccca tccgcttgat cttgccgcgg acgttgaaga
     1141 gccgcacttc ctggactttc actttgaaga gctcttcgat cgcgctcttg acctgaatct
     1201 tgttcgcctt cacgtcgact tcgaaggcca ggatgttgtc gccggcatcg cgcatcatcg
     1261 tcgatttctc ggtgacgagc gggcgccgga tgatctggtt cgcattcatg actcgagtgc
     1321 ctccgtcagc cgttgcagcg catcctggct gagaaccacg aagcgcgagt tgacgacgtc
     1381 gtacacggtg acgtgcagcg catcgacgag cgtcagcttc gggttgttcc gggctgccag
     1441 cgccaggttc gcgttctcga acgaatcgac gagaagcgct ttcccttcga cgccgatgcc
     1501 ggtgaacttc gcggccagcg cctgggtctt ggcctcatcg acggcgagat cattgagaag
     1561 gacgagcttc ccttccttga cccgctgcga gagcgccgac ttaagcgcgt tcttcttttc
     1621 cttcgcgttc atcttgtagg agtaatcgcg aggcgtggga ccgaaaaccg tgccgccatg
     1681 acgatggatc ggcgggcggg cgccgccctg acgtgcgcgg ccggtgccct tctgccggaa
     1741 cggcttcttg ccagagccgc tgacttccga gcggttcttg accttgtgcg ttccctggcg
     1801 aagcgaggcg aggtagttcc gcactgcttc gtggatgagg tgttctttgt acggataggc
     1861 gaagatcgct tccggaaggt cgaccgagcc caccttcttg ttgttccagt ctttgatgtc
     1921 gatggtcggc atgttattca ccccgcttcc cgttcctgga gaagcgaacg acgacgaggc
     1981 cgttctttcc gcccggaact gcgccgcgga tgtagagcag gttgttctct gcatccactt
     2041 tggcgacgcg gagattcttg accgtgacct gatcgccgcc catacggccg cccatgcgca
     2101 tgcccttcat gacacgcgac gggaacgccg aggcgccgat cgagccggga gcgcgatgga
     2161 acatcgagcc gtgagtggcc cggccgccgc cgaagtgatg acgcttcatc acgccctgga
     2221 agcctttgcc cttcgactgg ccgacaacgt cgatcgagtc gcccgccttg aagatgtcga
     2281 cctgcacgcg gtctcccacg ttggcgtcgc cttcgtacgg gaactcgacg agcgtccgaa
     2341 ccggcggaac gcccgccttc tcgaagtgcc cgcgcgccgc gctggtgatc cggcggttgg
     2401 agatcttctc gacgagaccg acctggacgg cgtcatagcc gtccttttcg ctcgtcttct
     2461 tctgtaccac gaggcaaggg ccggcctgaa tgaccgtcac cgggacgagt gctccgtcgt
     2521 cagcgaacac ctgggtcatt ccgaccttct tgccaatgat tccttcgatt gccatgtcgt
     2581 tcaccttgga accctttccc gggttcgtgt gtgctatttc ccgaacgcct tgatctcgac
     2641 gtctacacca gcgggcagct cgagcttcat gagcgcgtcg accgtctggg tcgtcggctc
     2701 gaggatgtcg agcagccgct tgtgcgtccg catttcgaac tgctcccgcg acttcttgtc
     2761 gacgtgcggg ctgcgcagaa ccgtgtaacg cgcaatctgc gtcggcagag gaatcggtcc
     2821 cgctacacgg gcgcccgttc gcttggccgt gtcgacgatt tcggaggtcg actggtcgag
     2881 aacgcggtag tcatacgctt tgagccggat gcggatcttt tcgttcatc
//
LOCUS       DATLVW010000903         9940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_534680, whole genome shotgun sequence.
ACCESSION   DATLVW010000903 DATLVW010000000
VERSION     DATLVW010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534680"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..575)
                     /locus_tag="VNN25_26740"
     CDS             complement(99..575)
                     /locus_tag="VNN25_26740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95201.1"
                     /translation="MRNLLIAAALVLAACSSSAPATPQQKANVPDPDFEIRQIVGPRE
                     QNADEGPLEVKYRLDIGNRADVPITLARVEMFTVNPPGGAYTLDVQHRTYYFRRAIEP
                     HQTASIEFWAKAYAYGAGPRENEPVTVRGTLYFESPNGYLRHVFVKELSQYPGQND"
     gene            731..3664
                     /locus_tag="VNN25_26745"
     CDS             731..3664
                     /locus_tag="VNN25_26745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXH95202.1"
                     /translation="MLDHRLAFERLISGISTNFINVDAAGVDREITAALESVGRFVGA
                     DRAYIYMFSDDGAWAVLTHEWNADPAVTRGLGEKVPFAAFPWTSGQLNDRRQLTISID
                     DLPGEAAAEREAYERAGNRSVAFVPMMYHHASLGALGVASTERRRWSEDAISLLRIIG
                     EIFVSAIQRHRTEAALRASEQQHRLLFERNLAGVYRTTIDGRILECNEALARILGFSS
                     RQELMERSARDLYQDPSHRDEYIAILRRERMMTSNEISLRRKDGRPCWLIESVHLLEG
                     DPEILEGTVIDITDRKLAENALRESEERYRLLVERMREGLTRTDNDGVLQFVNDRFCE
                     MVGYSRGELVGTKADLLLAYPEDVALLHQKTRLRIRHIADQYELRIRRKDGTVIWLEI
                     GGSPVVDAVGNVIGSIGVHNDITERRMAAEALRNSEARYRLMAENSTDMISRTSSRGI
                     ILYASDASRRLLGYEPSDLVGHSFYDFIFPPDREEVRHLSALINEAGPTTFAYRVEKK
                     DGSLVWFETTSRIILDVVSGEIREVVGVSRDITERKTVEEHIEYQAYHDALTGLPNRR
                     LFRDRLTVALAHARRLKHPLAVMFLDLDRFKVVNDTLGHSVGDEFLKAVAMRLQAALR
                     EEDSVARMGGDEFTVLLADLKTSNDAAKIAQKVLETVAQPLAIEGTELFLTTSIGIAL
                     FPSDGDTAETLLANADHAMYRAKDAGRNSYQMFTPAMNSRALERLSLESDLRHALGRE
                     ELELYYQPQINIATGRITGVEALIRWNRPGFGVVGPLEFIPVAEETRLIVPIGEWVLN
                     EACRQAKAWEQGRQSSFRMAVNLSPRQFQHSDLPQLIAAALELSGLAPSSLELEITES
                     LAMQNTARTIATLHRLREMGVQIAIDDFGTGHSSLNYLRSFPIDSVKIDQEFVQEIEA
                     SAADRAIVSAVIGMARGLRLRVTAEGVETERQLNFLREQGCEDVQGFLFGEPVPASAL
                     RPGQS"
     gene            4519..4785
                     /locus_tag="VNN25_26750"
     CDS             4519..4785
                     /locus_tag="VNN25_26750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95203.1"
                     /translation="MISWREIAISPKEIATSANEIGITQREIGTSEEEIGITSREIAI
                     SPKQIGRGQVETETDDTSLRTLRSMQPVVSFGFDLTPSDTANSA"
     gene            complement(4782..6449)
                     /locus_tag="VNN25_26755"
     CDS             complement(4782..6449)
                     /locus_tag="VNN25_26755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95204.1"
                     /translation="MKLLPLILLASLIVPRANAQWPQIGANPGHSGKVTVAAQPLLQL
                     MSDIVYDPFVAQEQAAFGGDLFVHYQVPLIDDDGVYMEFKSGTFTSPEHWETQDWSIH
                     GLRWQDGTLVDRWTAPSDWKPVPANGAAFFEPVFHSVLANGFLYMPGAGGTLMQIDRS
                     DGHLVTRINPFGSLDPNIYVSGVPAADAAGNIYYGAFRLDASNPWGKDITGAWLVKVQ
                     ADGNTRAIPFATLVPNAPKPTDQCTYAFSPGAPHPLPPSPSAVAPTIPCGSVRPGINA
                     APAIAADGTIYVAGVANFNPFWSYLVAVNADLTPKWSASLRNRFHDGCGVLIPYGTSS
                     GACRAGATLGVDPNDNQPGSGVVSDDSTSSPVVLPDGSVLYGTYTGYNGARGHLTHFS
                     SSGTFLISYDFGWDITPAVWEHDGTWSIVIKENFYAAPQKYFITQLDANLQREWQFKS
                     TNTLACGRGPDGNVFCADEQPNGFEWCVNAPAIDSRGVVYVNSEDGNLYAIGQNGIVQ
                     EKIFLQLALGAGYTPVSIGADGKIYAQNAGHLFAVGGSRPRVRAVRH"
     gene            complement(6446..6745)
                     /locus_tag="VNN25_26760"
     CDS             complement(6446..6745)
                     /locus_tag="VNN25_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HXH95205.1"
                     /translation="MRTTLTIDDQLTRELKEIALRTGRRFNAVVNETLLAGLQNLGTP
                     ARKPYRLETVSLGGLTKGFNLDKALQIADSLEDNEIARRLDASSHRRHRTSGNPK"
     gene            6855..7280
                     /locus_tag="VNN25_26765"
     CDS             6855..7280
                     /locus_tag="VNN25_26765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95206.1"
                     /translation="MKHAMLVAALLLSGCGTTEARLTLKLTSDGGFTGKGVGGVAIDG
                     TSIEASDMRRSCRGALRAEEESRLRELAAAARPEAWPESYASTDRPHGSPDQIRYTIT
                     VGAHSTSWYGEAGEGVPHEIMKLRDALVVVQQRVLKECP"
     gene            complement(7321..7572)
                     /locus_tag="VNN25_26770"
     CDS             complement(7321..7572)
                     /locus_tag="VNN25_26770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95207.1"
                     /translation="MNAKESLLKEAGYWYNFERMSYSNRLAKKVFAAETIEDNTEEWL
                     AERVAEPNTTGDWQFYDEPAGSVRRAFVAELENGRTAHS"
     gene            complement(8160..>9940)
                     /locus_tag="VNN25_26775"
     CDS             complement(8160..>9940)
                     /locus_tag="VNN25_26775"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HXH95208.1"
                     /translation="SIDSVMAIRTAATQLGAWDADIVDVPSLTYVVNEKSTAFLAWTA
                     KISFKDADGPELTQVYADASGSGDLVRWSGLFQRLKSRKVYTANNGTSLPGTLLWSEG
                     GTQTSDTTAQAAYNYAGNVYDYYKNVLGRDSYDNAGATIISSVHYSSSYNNAFWNGSQ
                     MVYGDGDGTTFIPLSKALDVDAHELTHAVTERTAALAYSNESGALNEATSDIMGNSCE
                     AYSKNAGTPNSNTWKVGEDIYTPNTAGDALRYMNNPTQDGSSKDWYPSRYTGTADNGG
                     VHSNSGIANLAYYLMVMGGTHPRSMSTVVVPALSATSSTSLDMAQRIWYRALTVYMTS
                     STNFQGARNATAQAAADLYGGTAGSAYNSVQKAWDCVGVPGGTGGGGGVTTLSNGQTV
                     TGIAGATGSWTYYKITVPASQASLKINMSGGTGDADLYVKLGAQPSATVYDYRPYLTG
                     NTESVSVTNPAAGDWYIGLNGYAAYSGVSLVATYTGGGGGGGCTTYSGTLSGTGASQI
                     QPNGTYYQTTVSGAQTAQLTGPASGADFDLYLYKWNGSSWAVVASSESSTANESINYS
                     GTAGYYEWKVLSYSGSGSYTLCISHP"
BASE COUNT         1888 a         2892 c         3180 g         1980 t
ORIGIN      
        1 acgagagatc agagatcaat agcgggggca gaactggagg ccagagacca gaaaggtact
       61 cctgacctcc gatctctggt ctctgatctc tgccccgatc aatcgttctg ccccggatac
      121 tggctgagct ctttcacgaa cacgtggcgc agataaccgt tgggcgactc gaaataaaga
      181 gtgcctcgca cggtgaccgg ctcgttttcc cgcggcccgg cgccgtaggc gtacgccttg
      241 gcccagaact cgatgctggc ggtctggtgc ggttcgatcg ctctgcgaaa gtaatacgtg
      301 cgatgctgga catcgagtgt gtacgccccg cccgggggat tcaccgtgaa catctcgact
      361 cgggcgagcg tgatcggcac gtccgcacga ttgccgatat cgaggcgata cttcacctcc
      421 aacggcccct cgtccgcgtt ctgctcccgc ggcccgacga tctgacggat ctcgaaatcg
      481 ggatcgggga cgttggcctt ctgttgcggg gtagccggtg ccgaggatga acaggctgcc
      541 agaacgagcg cggcggcgat gaggaggttt ctcatcctcc gattctagag caagaagcga
      601 tccgggcttc cgaaccagtg tgcagcaact cctgccggac ggtgctaacg taacgaattc
      661 accctgggag actcgattgt cggacgtgat ttcgctcact aaacagtctg gggcgcagtc
      721 tgcgcatgag atgctcgatc accgcctcgc cttcgagagg ctgatctccg gcatctccac
      781 gaacttcatc aacgttgacg ccgccggcgt cgatcgggag atcaccgccg cgctggagag
      841 cgtggggcgt ttcgtcggtg ccgaccgcgc gtacatctac atgttcagcg acgatggggc
      901 gtgggcggtg ctcacgcatg aatggaatgc cgatccagcc gtgacccgtg gcctcggcga
      961 gaaagttcct ttcgcggcat tcccctggac ttccgggcaa ttgaacgatc gccggcaact
     1021 gacgatctcg atcgacgatc tgccgggaga agcggccgcc gagcgggaag cgtacgagag
     1081 ggccggtaac cgctccgtcg ccttcgtgcc gatgatgtac caccacgcct cgctcggcgc
     1141 gcttggagtc gcttccacgg aacggcggcg ctggagcgag gacgccatct cgctgctgcg
     1201 aatcatcggc gagattttcg tcagtgccat ccagcgtcac cggacggagg cggcattgcg
     1261 cgccagcgag caacagcatc gtctactctt cgagcgcaat cttgccggcg tttatcgcac
     1321 cacgatcgac ggccgcatac tggaatgcaa cgaagccctg gcccgcattc tcggcttctc
     1381 ttctcgtcag gagttgatgg agcgctccgc gcgggacctc tatcaggatc cgtcgcatcg
     1441 cgacgaatac atcgccatcc ttcgccggga acggatgatg accagcaacg agatctccct
     1501 gcggcggaaa gacggccgcc cgtgctggct catcgaaagc gttcacctgc tcgaaggaga
     1561 tcccgagatt ctggaaggga ccgtcatcga catcaccgac cgcaagctgg ctgagaacgc
     1621 gctgcgggaa agcgaggagc gttaccgcct gctcgtcgag cggatgcgcg aaggactcac
     1681 caggacggac aacgacggtg tcctgcagtt cgtcaacgat cgtttttgcg agatggtcgg
     1741 ctactcgcgc ggagagctcg tcggcacgaa ggctgatctg ttgctcgcct atcccgaaga
     1801 cgtggccctg ttgcatcaga agacgcggct gcgaatacgc cacatcgccg atcagtatga
     1861 gctacgcatt cgacgaaagg atgggaccgt gatctggctg gagatcggcg gctcgcctgt
     1921 agtcgacgcc gtgggcaacg taatcggatc gatcggcgtg cacaacgaca tcaccgaacg
     1981 gcgcatggcc gcggaagcgc tgcggaatag cgaggcgcgc taccggctca tggccgagaa
     2041 ttcgaccgac atgatctcgc ggacttcgag ccgcggaatc attctctatg catcggacgc
     2101 gtcacgacgg ttgctcggat acgagccctc cgatctcgtc ggacactcgt tttacgactt
     2161 catcttcccg ccggaccgcg aagaagtccg tcatctctcg gcactgatca acgaggcggg
     2221 tccgacgacg tttgcctatc gcgtcgagaa gaaagacggc tcactggtct ggttcgagac
     2281 gacgagccgt atcatcctcg acgtcgtctc gggcgagatc cgcgaagtcg tcggcgtctc
     2341 ccgcgacatc accgagcgaa aaacggtcga agaacacatc gaatatcagg cgtatcacga
     2401 cgccctgaca ggacttccca accggcggct ctttcgcgac cggctcaccg ttgcgctcgc
     2461 ccacgcccgc cgcctgaaac acccgctggc ggtgatgttc ctcgatctcg atcgcttcaa
     2521 ggtggtcaac gacaccctcg gacattcggt cggcgacgag tttctgaaag ccgtggccat
     2581 gcgcctgcag gccgcgctcc gcgaagaaga ttcggtggcc cggatgggag gcgatgagtt
     2641 caccgtgctc ctcgccgatc tcaagacgag caacgatgct gcgaagatcg cgcagaaggt
     2701 gctcgagact gtcgcgcagc cgctggccat cgagggaacc gagctctttc tcacgaccag
     2761 catcggcatc gcgctctttc cgagcgacgg cgacacggcc gagacgctgc ttgcgaatgc
     2821 cgaccacgcc atgtatcggg ccaaggatgc cggccggaat tcctatcaga tgttcactcc
     2881 ggccatgaac agccgggccc tggaacggct gtctctggag agcgatctgc gtcacgcgct
     2941 cggacgcgag gagctggagt tgtactacca gccccagatc aacattgcca ccggccggat
     3001 caccggcgtc gaggcgctca tccgctggaa ccggccgggt tttggagtgg tgggaccgtt
     3061 ggagttcatt cctgtcgccg aggagacaag gctgatcgtg ccgatcgggg agtgggtgct
     3121 gaacgaagcc tgccgtcagg caaaggcgtg ggagcagggc cgtcagtcct cgtttcgcat
     3181 ggccgtcaac ctctctcctc ggcaattcca gcactccgat ctcccgcaac tgatcgccgc
     3241 agcgctcgag ctctccggat tggcgccgag tagcctggag ctggagatca cggagagcct
     3301 ggcgatgcag aacactgccc gaacgattgc aacgctgcac cgcttgcggg agatgggtgt
     3361 gcagatcgcc atcgacgact tcggcaccgg ccactcctcg ctcaattacc tgcgaagctt
     3421 cccgatcgac agcgtgaaga tcgatcagga gttcgtgcag gagatcgaag cgagtgccgc
     3481 cgaccgcgcc atcgtttccg ctgtcattgg catggctcgc ggtctccgcc ttcgcgtgac
     3541 ggccgaaggc gtggagaccg agcgccagct caatttcctg cgcgaacagg gatgcgaaga
     3601 cgtgcaggga ttccttttcg gcgagccggt gcctgcaagc gcgctgcggc cgggacagag
     3661 ctgacgatcc gttctcgcgg ttcgtgagtt tccaattccg agagcgaaac gatgttcaaa
     3721 cgtgcgggac tcctttcgca ggtgccgacc cagggcgaca tccgcagcgc cttcggaggc
     3781 ctcgcgatct cgaaacccca taccgatccg agacttggag gggtcgtttt gagatcgagg
     3841 ggcgtctttt taagaatcgg aggggtcgtt ttaagaatca ggggggtcga tttgagactc
     3901 ggagggggca atttgagatt gggaggggtc gcgatctcga aaaaggagat cgcgattcgt
     3961 tctcgcgaga tcgctttggg aaacctatag atcgctttgc gatttggaga ggtcgtttta
     4021 agaaaaaatc ccattctttt gagatcgggc ggggttgact tgagaaatgg aggagtcgaa
     4081 acgagattcc ggggggtcgt tttaagaatg ggtgtggtcg ttcagagctt cgggggggtc
     4141 gttttgagat cgggaggggt cgttttgaga ttgagagggg tatcgatatc aaaaatggaa
     4201 atcgcgatct cttcggagga aatcgcgatt tcggggaagg agatcgcgat ttcggggaag
     4261 gtgatcgatc tgaggttccg agtggtcgat ttgagatttc gcgaggtcgt ttcaagagac
     4321 cgatagatcg acttgcgatc gagagggatc gttttaggaa accggcagat cgttttgaga
     4381 tcaggagaga tccttttgag aaatggcggg gtcgaaaggg gacttcgcgt ggtcggttca
     4441 agaaaagggg agatagcgat ctaaaaaatg gagatcgcga taagaagggc gggaatcggg
     4501 acttcggaga aggagatcgt gatctcgtgg agagagatcg cgatctcgcc gaaggagatc
     4561 gcaacctcgg cgaacgagat cgggatcacg cagcgggaga tcggtacctc ggaggaggag
     4621 atcgggatca catcgaggga gattgcgatc tcgccgaagc agatcggaag gggtcaggtc
     4681 gaaaccgaaa ctgacgacac ctcgctacga acactccgct cgatgcaacc cgtcgtcagt
     4741 ttcggtttcg acctgacccc ttccgatacg gcgaacagtg cctaatgccg cactgcccgt
     4801 acgcgcggcc gggagccgcc gacagcgaaa aggtgtcccg cgttctgcgc gtagatcttt
     4861 ccgtcagcac cgatcgacac gggggtgtag ccggcgccga gggcgagttg gaggaagatc
     4921 ttctcctgga cgatgccgtt ctggccgata gcgtacagat tgccgtcctc gctgttcacg
     4981 tacacaactc ctcggctatc aatcgccggc gcgttcacgc accactcgaa gccgttcggc
     5041 tgctcgtcgg cgcagaacac attgccgtcc ggcccgcggc cgcaggccag ggtgttcgtg
     5101 ctcttgaatt gccactccct ctgcagattt gcatcgagtt gggtgatgaa gtacttctgg
     5161 ggagccgcgt agaagttctc cttgatgacg atcgaccacg tgccgtcgtg ctcccacacc
     5221 gcgggcgtaa tgtcccagcc gaaatcgtag gagatcagga acgttccgga cgaggagaag
     5281 tgcgtcagat gtccgcgcgc gccgttgtag ccggtgtagg tgccgtacag aacggagccg
     5341 tcggggagga cgaccggcga cgatgtggag tcgtcgctca cgactcccga gccgggctga
     5401 ttgtcgttcg gatcgacacc gagagttgcg ccggcgcggc atgcacccga ggaagttcca
     5461 tacgggatga gcacaccgca tccatcatga aaacgattgc gcagcgatgc gctccatttc
     5521 ggcgtgagat cggcgttgac cgcgacgagg tacgaccaga agggattgaa gttcgcgaca
     5581 ccagcaacgt agatcgtgcc gtcggcggcg atggcaggcg ctgcgttgat gccgggcctt
     5641 acggaaccgc atgggatcgt cggtgccacc gcggacggag atggtggcag tgggtgcggc
     5701 gcgccggggc tgaacgcata ggtgcactgg tccgttggct tcggcgcgtt cggaacgaga
     5761 gtggcgaagg ggatcgcgcg cgtgttgccg tccgcctgta ccttcaccag ccaggcgccg
     5821 gtgatgtcct ttccccaggg attcgaggca tcgagccgga acgcgccgta gtagatgttt
     5881 cccgccgcat cggcggcagg aacgccggag acgtagatgt tcggatcgag tgagccgaat
     5941 ggattgattc gcgtcaccag gtggccatcg ctccggtcga tctgcatcag cgtgccgccg
     6001 gcgcccggca tgtagagaaa gccgttcgca agcaccgagt gaaacaccgg ctcgaagaag
     6061 gcagcgccgt tcgccggcac cggcttccaa tcgctcggcg ccgtccagcg gtccaccagc
     6121 gtgccgtcct gccaccgcag gccgtggatg ctccagtcct gcgtctccca atgttccggc
     6181 gacgtaaacg tgcctgactt gaattccatg tagacgccgt cgtcatcgat gagcggcacc
     6241 tggtagtgaa cgaagaggtc gccgccgaac gcagcctgct cctgcgcgac gaacgggtcg
     6301 tagacgatgt cggacatgag ctgcagcagt ggctgcgccg cgaccgttac cttcccgcta
     6361 tggcccgggt ttgcaccgat ctgcggccac tgtgcgtttg cgcggggaac gatgagggac
     6421 gcgaggagga tcagtggcag gagtttcatt tgggattgcc gctggttcga tgtctccgat
     6481 gcgatgaagc atcgagtctt cgtgcgatct cgttgtcttc cagcgaatcc gcgatttgca
     6541 gggctttatc gaggttgaag cctttcgtca gtcctccgag cgagaccgtt tcgaggcggt
     6601 aaggtttccg cgcaggtgtg cccaggttct gtagtccggc gagaagcgtt tcgttgacga
     6661 cagcgttgaa gcgtctgccg gttcgaagcg cgatctcttt gagctcacgg gtcagttgat
     6721 catcaatcgt cagagttgtt cgcatgaagc acctcgcgca ctgaatactc tgcctcaaac
     6781 actacgacgt cgagaattgc ttcctgattg ccgggcacag atgctgcgtc catgtgagag
     6841 cgacgaggta tcgaatgaaa catgcgatgt tggtggccgc cctgctactg tcaggttgcg
     6901 gtacgacgga ggcgaggttg acgttgaaac tgacgagcga cggcggattc accggcaagg
     6961 gagttggcgg cgttgccatc gacggcacga gcatcgaggc cagcgatatg cggcgctctt
     7021 gccgtggcgc gctgcgcgcc gaggaagagt cgcggctgcg ggagctggcc gcggcggcac
     7081 gtcccgaagc atggccggag tcgtacgcca gtaccgaccg tccgcatggt tctcccgacc
     7141 agattcgtta cacgatcacc gtcggggcgc actccacgtc ctggtacggc gaagcgggag
     7201 agggagtgcc gcacgagatc atgaagttgc gcgacgctct cgttgtggtg cagcaacgcg
     7261 ttctcaaaga atgtccataa cgcaagaaac ccttggttgt tgcggctctg cagccggttc
     7321 tcaactgtgc gcggttcgcc cattctccag ttcggcgaca aacgcgcgtc gtactgagcc
     7381 ggccggttca tcgtagaact gccagtctcc cgttgtgttg ggctccgcga ctctctcagc
     7441 gagccattct tctgtattgt cctcgatcgt ttccgcagcg aacactttct tcgcgaggcg
     7501 gttgctgtag gacattcgtt cgaagttgta ccagtagcct gcttccttca ggaggctttc
     7561 tttcgcgttc atccgatcga ttctagtccc catttctcac acctttcagc aaggtgctgc
     7621 cgccaatccc ctacaagtat gagaatttca gggtcagggt gagtttcatc ccgactgatc
     7681 gcgcaatcga gattgtgtca tcctgagact gccggagccg cccgcgcgaa tcgcgatgtt
     7741 tgcgcagtgc gtgctcggcg gcgtaggacg tcgaaggatc tcaaacttcg cagatcgtgt
     7801 tcgtccagcg cgagcgatcg tgatcaataa cgctcgaacg agatcttcgc aacttgagat
     7861 ccttcgaccg tcctacgccg ccgatgcctg ccgggtgcga gcgtcgtgaa acttgcggcg
     7921 gcttcggcgg ctcaggatga cacacaacgt cttgcacgta tcggcaatag cgtcgccgga
     7981 tcagaatggc gcgatcgcgt catcacacct ctcggtaatg cgccgccgcg gcctgtgctc
     8041 caactagctg cggccttcac gtcttcgctt gagcaagcag agtgctccac gccgaagacg
     8101 caaaagcccg ccgcgcgttg cgcgcggcgg gccctttgtt cgatgcaaag agacgttgac
     8161 tacggatggc tgatgcagag cgtgtagctg ccgctgccgc tgtacgagag aaccttccac
     8221 tcgtagtagc cggccgtgcc gctgtagttg atgctctcgt tggccgttga gctttccgag
     8281 gatgccacca ctgcccagga cgaaccgttc cacttgtaga gatagagatc gaaatcagcg
     8341 ccgctggccg gcccggtgag ctgggctgtc tgcgcgccgc ttacggtcgt ctggtagtac
     8401 gtgccgttcg gctggatctg cgatgcgccc gtaccgctga gcgtgccgct gtaggtcgtg
     8461 cagccgccac caccaccgcc gccggtatac gtggcaacca gcgagacgcc gctgtacgcc
     8521 gcataaccat tgagtccgat gtaccagtcg cctgccgcag ggttggtgac cgaaacgctt
     8581 tcggtgttgc cggtgagata cggacgatag tcgtaaaccg tggcagacgg ctgcgccccg
     8641 agcttcacat agagatcggc gtcacctgtg ccaccggaca tgttgatctt gagcgaggcc
     8701 tggctcgctg gaaccgtgat cttgtagtag gtccagcttc cggttgcgcc ggcgatgccc
     8761 gttacggtct ggccgttcga aagcgtcgtt acaccaccgc cgccgcccgt gccgcccgga
     8821 acgccaacgc agtcccacgc cttctggacg ctgttgtacg cggagccagc ggtgccgcca
     8881 taaagatcgg ccgccgcctg cgcggtggcg ttacgtgcac cctggaagtt cgtcgatgac
     8941 gtcatgtaga cggtcagcgc gcgataccag atgcgctggg ccatatcgag cgacgtagac
     9001 gatgtcgccg aaagtgccgg cacgacgact gtgctcatgc tgcgcgggtg cgtaccgccc
     9061 atgaccatga ggtagtaggc gagattggcg atgcccgagt tggagtggac gccgccgttg
     9121 tcagcggttc cggtgtagcg cgacggatac caatccttcg agctgccgtc ctgcgtgggg
     9181 ttgttcatgt agcgaagcgc gtcacccgcg gtgttcggcg tgtagatgtc ttcgccgact
     9241 ttccaggtgt tcgagttcgg tgtgccggcg ttcttcgaat acgcctcgca cgagttgccc
     9301 atgatgtcgg aggttgcttc gttgagtgcg cccgactcat tcgagtaggc gagagcggcg
     9361 gtgcgctcgg tgacggcgtg cgtcaattcg tgcgcatcga cgtcgagtgc tttcgacagg
     9421 gggatgaacg ttgtgccgtc gccgtcgccg tacaccatct gcgaaccgtt ccagaaggcg
     9481 ttgttgtacg acgatgaata gtgaacggac gagatgatcg tggcgccggc gttgtcatac
     9541 gagtcgcggc cgaggacgtt cttgtaatag tcgtagacgt tgccggcgta gttgtaggca
     9601 gcctgcgcgg tcgtgtccga cgtctgcgtt ccaccctcgc tccacagcag cgtgcccgga
     9661 agcgatgtgc cattgttcgc ggtgtacacc ttgcggctct tgaggcgctg gaacaagccg
     9721 ctccagcgaa cgagatcgcc gctgccggat gcatcggcat agacctgcgt cagctcgggg
     9781 ccgtccgcat ctttgaacga gatctttgca gtccatgcca ggaaggccgt gctcttctcg
     9841 ttgacgacgt acgtcagtga cggtacatcg acgatgtccg cgtcccacgc gccgagctgc
     9901 gtcgccgcgg tccggatggc catgacggaa tcgatgctcg
//
LOCUS       DATLVW010000904         4887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_37458, whole genome shotgun sequence.
ACCESSION   DATLVW010000904 DATLVW010000000
VERSION     DATLVW010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37458"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VNN25_26780"
     CDS             <1..569
                     /locus_tag="VNN25_26780"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HXH95209.1"
                     /translation="LGVATARWAADYYRMSLAETTVAIESLVRDGELLPARIESVAGD
                     AFLHRDHLPTLAAIRRGRRPSHATLLSPFDPVVWDRKRALALFGFDYRIECYTPAPKR
                     RYGYFTLPILVGDRLVGRLDAKAHRVAGIFEVKSLHLEEGIAIDDALAEKIAAAIHAC
                     AAWHGTPRVQLRRVVPREAAAILRSALG"
     gene            complement(604..957)
                     /locus_tag="VNN25_26785"
     CDS             complement(604..957)
                     /locus_tag="VNN25_26785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95210.1"
                     /translation="MSLFTPAVVVILALAALCALLAFRLRSLFIKYPLLLVATALLVL
                     AIWLGASDAWAFRDGFPLPAPGYAWSETHGLAAAKAFLLGFWVSLLGAVAVAALIVAS
                     WRHPASPGTAQHSQH"
     gene            complement(1322..1807)
                     /locus_tag="VNN25_26790"
     CDS             complement(1322..1807)
                     /locus_tag="VNN25_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95211.1"
                     /translation="MAGVGSRTEADAIADLERWFSRLRTGTYKVTSAYDRKYNCAAFA
                     AGQTDTWWEPAPDGYWPPGVPSEYTVDAYAAAYGSIGFVECDTATSERGFEKIAIYGD
                     NGDFVHAARQLPSGVWTSKLGRAEDIEHETLQQLEGNGQWEYRFVVKFMKRRWTTKAD
                     G"
     gene            complement(2196..4385)
                     /locus_tag="VNN25_26795"
     CDS             complement(2196..4385)
                     /locus_tag="VNN25_26795"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HXH95212.1"
                     /translation="MRFVVQTPLPIDDDVAAIVAHVRAHRSAVVVAPPGSGKTTRVPP
                     ALTAIGRTILLQPRRVAARALARRIASERGWTIGNEIGWQIRFERRFSERTRLLVATE
                     GILTARMQSDPLLSDFDVIVLDEFHERSIHADVALAMAKQAMEARPDLAVVVMSATIA
                     ASDVSQFLGGARVFEVGTRRFPVDVVYRPGVSVAGAVREQLRNPAGDILCFLPGMREI
                     ERAAAELANADALVLPLHGSLDVDAQERALAPSPRRKVILATNIAETSLTVEGITDVI
                     DSGLHKVLRFDPDTAVDHLVAERIAGDSADQRAGRAGRTQPGRAIRLWDARDILRPHR
                     EPDIGRVDLAPSLLDIVAWGGDPRTFAWFERPDEERIEAGMELLTSLGAIDSGRLTPA
                     GMTLRSLPLHPRLGRILIDAHGADEAVTIVAKLGGGGPPEERELASMSRKLLGDDYRQ
                     RIDDATLRRALLAGYPDRVAQRREPKSARVLLSSGTGATLAREIDDGNGEFLVVLDIT
                     GDLVRMARVVEREWLSPTRKEIVHELEGNRVQAIERSWYGAILLHEQRVAPEHAEAER
                     ILAEHAKPEEMLVRRVAFAQLDVDWPSLIAIAVAGKRSLDQVQIELPFPLRRKLDELA
                     PLTIPLPSGRSARLEYRDDGSVLASAKLQELFGLAESPRLGPHRTPITFALLSPGGRP
                     VQITQDLRGFWNGAYQEVRKELRGRYPKHPWPEDPWTAPATHRTKRR"
     gene            4387..4638
                     /locus_tag="VNN25_26800"
     CDS             4387..4638
                     /locus_tag="VNN25_26800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95213.1"
                     /translation="MATKRDDDGIDYTAEGTTPPHEKNDPTNAGYDEAAHKGGAYGLK
                     EGEGGVFGTSGGGTYRGGLHVEDRDAVVNHSKKNEPQRH"
     gene            complement(4654..>4887)
                     /locus_tag="VNN25_26805"
     CDS             complement(4654..>4887)
                     /locus_tag="VNN25_26805"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HXH95214.1"
                     /translation="KEMARELLTTAELQSVIRRQGFDGIHEIETCILEPGGAFSIKGK
                     SPSADVKRHDELLHRLDALTAQIAAMEKKMAGG"
BASE COUNT          866 a         1559 c         1605 g          857 t
ORIGIN      
        1 cgctcggcgt cgctactgcc cgctgggccg cggactacta ccgcatgagc ctcgccgaga
       61 cgacggtggc gatcgagtcg ctcgtccgcg acggcgagct ccttcccgcg cgcatcgaga
      121 gcgtggctgg cgacgcgttt ctccatcggg accatctccc gacgctggct gcgatccgcc
      181 gcggccgccg tccctcgcat gcgacgctcc tctcgccctt cgatccggtg gtctgggacc
      241 ggaagcgcgc cctggcgctc ttcgggttcg actaccgcat cgagtgctac acgccggcgc
      301 cgaagcggcg ctacggctat ttcacgctcc cgatcctcgt tggcgaccgg ctggtcggcc
      361 gcctcgacgc gaaggcccat cgcgtcgccg gcatctttga ggtgaagtcg ctccatctcg
      421 aagaaggcat cgccatcgac gacgcgctcg cagagaagat cgccgccgcg atccatgcct
      481 gcgccgcctg gcacggcacc ccgcgcgtcc agctgcggcg cgtcgtgccg cgagaggccg
      541 ccgcgatcct acgcagcgcc ctcggctgac gcgaagcgga agcaatctgc cagccttgag
      601 caatcaatgc tgactgtgtt gcgcggttcc gggcgaagca gggtgccgcc agctcgctac
      661 gatcaacgcg gcgacggcga cggcaccaag tagtgatacc cagaagccga ggaggaacgc
      721 cttggcggcg gccagcccgt gcgtctcgga ccaggcgtaa ccgggcgccg gaagtggaaa
      781 gccgtcgcgg aacgcccacg cgtcgctggc gcccagccag atcgccagaa ccaggagagc
      841 agttgccacg aggaggagcg ggtatttgat gaacagggag cgcagtctga aggctaagag
      901 cgcgcaaagg gcggccaacg cgagaatcac gacaacagcg ggcgtgaaga ggctcatgtc
      961 gttctcaaga ttcggaacca gggattcatt gaaccacgga gcgggggatg aaggatgagg
     1021 gaagaagtcc tttttcctcc tcctctgtgt cctctgtgtc tctgtggttc aaccggcttt
     1081 gaatagcaca atcacggccg cctcttgctc aacgagaggc aacgtggcgc atcgtgggtt
     1141 ccggcgcgtt cggcggaaga gttacgacgc cttcggtatc gtgcttctca cgctctcgga
     1201 ggtactcgaa gaacgcgtga atgctttcac ctgtttgctc tacaagttcc gcgcgagctg
     1261 catgagtctc ttcgatgatt ggatcttccc acatctcagg ctcccatgag ctcctcacgt
     1321 ttcacccatc cgccttcgtc gtccagcgcc gtttcatgaa cttcacaaca aaacggtact
     1381 cccactggcc gtttccttcc agttgctgca gtgtttcgtg ttcgatgtcc tccgcccgtc
     1441 ccagcttact cgtccataca cccgatggaa gttgacgagc cgcgtgaacg aagtcgccgt
     1501 tgtctccata gatcgctatc ttctcaaagc ctctctcgga ggtggcggtg tcgcattcga
     1561 caaacccaat actcccgtat gccgcagcgt acgcatcaac ggtgtattcc gaaggaacac
     1621 caggaggcca gtaaccatca ggcgccggct cccaccaggt atcggtttgg cccgccgcaa
     1681 acgccgcgca gttgtatttc cggtcgtacg cgctcgtcac cttgtaggtg ccagtacgta
     1741 gcctggaaaa ccagcgctcg aggtctgcta tggcgtcagc ttctgttcgc gaccccacgc
     1801 cagccacgcc tccgccattc ttccgatctt accggcgtct cgcggatcga cagggtccac
     1861 accggtgatt gcccgtaacg cccagaacca cggatcagca tttactgcga gacggtcaag
     1921 gataagcggg agcacggggc ggcccatgcc aatgattcgc tgataactgg gatgcaggac
     1981 agtctgtcga atactcgatg aattcgcggt atcagcgagc caccgctcgg tgtgagcttg
     2041 aaactccttt attagcgacg ctgagtcgcc ttcaggctcg gagacttctg gaagcttcag
     2101 tgttgatgac acgcactcac ctactgaaat catagcgcaa caagtcacaa cctgatcttc
     2161 ccccagcgtt attgccccac tcttgtcttt tgctcctatc gccgcttcgt ccggtgcgtc
     2221 gcaggtgccg tccacggatc ctccggccat gggtgcttcg ggtaacgccc gcgcagctct
     2281 ttgcgcactt cctggtacgc gccgttccag aagccgcgga ggtcttgcgt gatctggacg
     2341 gggcggccgc cgggggagag gagggcgaag gtgattgggg tgcggtgggg gccgaggcgg
     2401 ggggattcgg cgaggccgaa gagctcttgc agtttggccg aggcgagtac ggagccgtcg
     2461 tcgcggtact ccaaacgggc gctgcggccg ctcggcagcg ggatcgtgag cggcgccagc
     2521 tcgtcgagct ttcggcggag cgggaagggg agctcgatct gaacctggtc gagacttcgt
     2581 tttccggcga cggcgatggc gatcaacgaa ggccaatcga cgtcgagctg cgcgaaggcg
     2641 acgcgccgca cgagcatctc ctcgggtttc gcgtgctccg cgaggatccg ttccgcctcg
     2701 gcatgctccg gtgccacgcg ttgctcgtgc agcaaaatcg cgccgtacca gcttcgctcg
     2761 atggcttgaa cgcggttccc ttcgagctcg tgaacgattt ctttccgagt cggggagagc
     2821 cactcgcgct cgacgacgcg ggccatgcgc acgagatcgc cggtgatgtc gaggacgacg
     2881 aggaactcgc cgttgccgtc gtcgatctcg cgagccagtg tcgcgccggt gccggacgac
     2941 aacagcacgc gggctgactt cggctcgcgg cgttgggcca cgcgatcggg atagccggcc
     3001 aggagagcgc gccgcaaggt ggcgtcgtcg atgcgctggc gatagtcgtc gccgaggagc
     3061 ttgcgggaca tcgaagccaa ctcgcgctcc tcgggtggcc cgccgccgcc gagtttcgcg
     3121 acgatcgtca ccgcttcgtc cgcgccgtgc gcgtcgatga gaatccggcc gaggcgcgga
     3181 tggagcggca gcgagcgaag cgtcatgccg gcgggcgtca ggcggccgct gtcgatggcg
     3241 ccgagggagg tgagcaattc cattccggct tcgatgcgct cttcatccgg ccgctcgaac
     3301 catgcgaacg tgcgcgggtc gccaccccag gccacgatgt cgagcaggga aggagcgaga
     3361 tcgacgcggc cgatgtcggg ctcgcgatgg gggcggagga tgtcgcgcgc atcccagagc
     3421 cggatggcgc gtcccggttg cgtgcggcct gcgcgtccgg cacgctggtc tgcagaatca
     3481 ccagcaatgc gctcggcgac gagatgatcg acggcggtgt cgggatcgaa acgcaacacc
     3541 ttgtgcaggc cggagtcgat gacgtcggtg atcccttcca cggtcagcga cgtctcggcg
     3601 atgttcgtcg ccaggatgac tttgcgccgc ggcgaagggg cgagcgcacg ctcctgtgcg
     3661 tcgacatcga gcgagccgtg caacggcagg acgagcgcgt cagcattcgc aagctccgcg
     3721 gctgctcgct cgatctcgcg catgccgggc aggaagcaga ggatgtcgcc agcaggattg
     3781 cggagttgct cgcggacggc accggcaacg gagacgccgg ggcgataaac aacatcgacc
     3841 ggaaagcggc gcgtgccgac ttcgaacacg cgggcgccgc cgaggaattg ggagacgtca
     3901 gaggcggcga tggtggcgga catgacgacg acggcgaggt cgggacgcgc ctccatcgcc
     3961 tgcttcgcca tcgccagcgc gacatcggcg tgaatgctcc gctcgtgaaa ttcgtcgagc
     4021 acgatgacgt cgaaatcgct cagcagggga tcgctttgca tccgcgccgt cagaatcccc
     4081 tcggtggcca cgagcagccg cgtccgctcg gagaagcgcc gctcgaaacg gatctgccag
     4141 ccgatctcgt tgccgatcgt ccagccgcgc tcgctggcga tgcgccgtgc cagcgctcgt
     4201 gcggccacgc ggcgcggctg cagcaggatc gtccggccga tcgcggtcag ggcaggaggg
     4261 acgcgcgtgg ttttgccaga gccgggcggc gcgacgacga ccgcgctgcg atgcgcgcgg
     4321 acgtgcgcga cgatcgcggc cacgtcatcg tcaatcggga gcggcgtttg caccacgaac
     4381 cgcattatgg cgacgaaacg cgacgacgat ggcatcgact acaccgcgga agggacgacc
     4441 ccgccgcacg agaaaaacga cccgacaaac gccggctacg atgaggccgc tcacaaaggc
     4501 ggcgcgtacg gcctgaaaga aggtgagggc ggcgtcttcg gcacgagcgg cggcgggacg
     4561 taccgcggcg ggctgcatgt cgaggatcgg gacgctgtgg ttaaccattc aaaaaagaat
     4621 gaaccacaga gacactgagg gcacagagag ctcttatcct cctgccatct tcttctccat
     4681 cgcggcgatc tgcgcggtca gtgcgtcgag gcggtgcagc agctcgtcgt ggcgctttac
     4741 atccgccgac ggcgactttc ccttgatact gaaagcgccg cccggctcga ggatgcaggt
     4801 ctcgatttcg tgaatcccgt cgaagccctg gcggcgaatg acgctttgca gctcggccgt
     4861 cgtgagcagc tccctggcca tctcttt
//
LOCUS       DATLVW010000905        14920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_122698, whole genome shotgun sequence.
ACCESSION   DATLVW010000905 DATLVW010000000
VERSION     DATLVW010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122698"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VNN25_26810"
     CDS             <1..1425
                     /locus_tag="VNN25_26810"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXH95215.1"
                     /translation="IDSLSQGRIVTIGGDPICASRPLPLFYSRRQFAPAWSARDRDDL
                     LAAIRDAKNDGLDPADYHLAAIERTSDPEQRDLLLTDAFFLLASHLLSGRVDPESIEP
                     TWCLTPRGNDLVAALETALELHDVRAAIARMPPAHPEYAALRRWLATYRRIAAAGGWP
                     DIDEPRDAKKSLRLGDRGPMVAQLAARLVASGDLAQASDEFDASLDHAVRRFQSLQGL
                     SADGVVGPRTRAELNIPAAARVRQIELNLERWRWLPSDLGDPHALINIPAFSLVLTEH
                     DRTVMSMRIVVGKDYLRTPIFSGNITQVVLSPDWNVPDSIAEKELWPKEHRTPGYLAR
                     EHIEVLRGGKLRQTPGPWNSLGLIKFNIPNKYDVYLHDTPAKSLFEASARAFSHGCMR
                     IEKPVDLAEWLLRDRPEWTRDRIVQESQRGIETTIPVTKPLPVHVLYWTAFVDESGEL
                     HFAPDVYHRDGRLDAAMRQAPSRF"
     gene            1573..2001
                     /locus_tag="VNN25_26815"
     CDS             1573..2001
                     /locus_tag="VNN25_26815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH95216.1"
                     /translation="MRIQDVMSKRVVSIAPKATISDARETLRTVEIDHLVVVSGKQIV
                     GVIAGKDLARAGDEQPVENAMTRDVVTIEPEATLRHAAGVMRGRAIGCLPVVDDERLV
                     GIVTTSDLLTALAKGDTHTSPPSERVTLRKRGPRKRAATI"
     gene            complement(2020..3417)
                     /locus_tag="VNN25_26820"
     CDS             complement(2020..3417)
                     /locus_tag="VNN25_26820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXH95217.1"
                     /translation="MRNIEHAATLAFDVLNALSDNIAVLDSRGVIVAVNDAWIRFASA
                     NGAADERFYVGTDYLSVCEDALARGEDGPLERMTDGLRSLLAGNSDEVSMEYPCDSPT
                     EKRWFALKVTRLRGESGAAAVVSHADITRRKRIEQSHREAERLLRTVLEALPVGVWIQ
                     DSGGRIVHGNPAGQQIWMGARFVGPEEFGQYKGWWLSTGLPVAAEDWAAARAIRNGET
                     SIGEEIEIECFDGTRKIILNSAVPLVGESGSIEGAIIVNQDISAQKRDEAVIMRAKES
                     VEATSRELAAALDRERTLGRIDDLTGLTNRRHFFDLAVHAVSEAARYHHGLALVMFDI
                     DNFKHTNDTLGHQAGDLLLKRIAKVGRTHLRESDIFARYGGDEFVILLPHTHAHEAAL
                     VSDRMRGDLSAQSIVMKQDVVTVTISSGIAELLPQNDSLDALIHRADIALYQAKNEGR
                     NRTKLFMGGEDLRLF"
     gene            complement(3553..4248)
                     /locus_tag="VNN25_26825"
     CDS             complement(3553..4248)
                     /locus_tag="VNN25_26825"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH95218.1"
                     /translation="MPSQPCTTCSAPRSFCSLSEELHAVFESLKTSVTFAKGEAAFHE
                     ADPCHSVFVVCAGSMKLVASSSEGKVLLLRFAGPGTLLGLAEAMLERRPYQCSAIAAE
                     ASALSVIPAETFVRFVTTYPEACLRLTVALSEHYKVAQRETKFLAMGGSSAARLAHLL
                     LEEASAHGILAEDGIHVPLHITHSEIGQSIGTTRETVTRILGELDHSGLIERTADAII
                     IHGTEELTRLAAY"
     gene            5364..5966
                     /locus_tag="VNN25_26830"
                     /pseudo
     CDS             5364..5966
                     /locus_tag="VNN25_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008975.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
     gene            6337..8271
                     /locus_tag="VNN25_26835"
     CDS             6337..8271
                     /locus_tag="VNN25_26835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95219.1"
                     /translation="MKRHFPILALIAACIALALIALAYSNFFHNSFHFDDDHVIVENA
                     SIKSLSHWTRFFTDAHTFSSQPSNATYRPIVTLSFALDYAVHGSLDPVPFHVTQLLLL
                     IIVGVLLSIVYTSLFGPGQETLAVIAAGIFCLHTANTETMNLLSSRSELLSAIGLLAA
                     LIVFIRWPALRRRGLYLIPLAIGALAKAPVVIFAGIVMAWVWLVERRRPTDALKAALP
                     SFITGIALLIGLNAMNAKEWIAGGGSRIGYLITQPYAWLHYARLVILPAGLSADTDLQ
                     PMAHWYDTNAIAGYAFVALLCLAIARFARTREEAPIAFSLAWFGITLLPTSSVFPLAE
                     VVNEHRLFFPLMGAAAAFVWTAELIVRRWPQAQRLAYSVVVISALALAYGTHARNEAW
                     HTEETLWRDVTQKSPRNGRGWMNYGLTQMEVGRYISAKEAFDRAAQFTPSFGTLEINR
                     GIVAAALGDNVAAEQHFRRALELHPDRNAHFYYARWLLRRGRGTEAEPHLAEAAKIAP
                     TWVPPRRLALQVAVGRGQDSKARALAAGILQIDPTDAEALAVQRDGADLRGGTYRACF
                     DQAWASMRDDQHALAASGFRAAIRFEPKATAYNNLGWSLASLGFRDDAAVAYQAAITA
                     DPQFERARNNLRDLDASTTR"
     gene            8252..9667
                     /locus_tag="VNN25_26840"
     CDS             8252..9667
                     /locus_tag="VNN25_26840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXH95220.1"
                     /translation="MPQQLGDYKRLRNLTGTQRWLLAAILIIAAALRIGWNNVETYSP
                     ADETVYANYTRTLVERGFFRGYPEIAQTFVRDPQRWNFPSPLRWGYFGLSTLAAHIKG
                     STEPRALAWLSTIAGILVVPLIFVFSLRLFDARVALLAAAFTAVSGIELALGRRGLQD
                     EVFCLSVLASTYCVMVIVTQRDDETPRWFIALAIASMTMSFAIKESFLYLIPAFAALL
                     LLHRRPRELRLLHVALFALPPFLYFAGFCLLTRSVGTFFRVGELVTSAMKAPYVVQYQ
                     SGPPHRLLLDFFITTPLVSILAAAAIVFIVLTASKRGSERSIALFVVVAVAVFAIVPS
                     KNLRFHVMLDPFFRLLAAWVIASSAVTANACKAWALAGVAAANAAIEIEIFHTIFIKH
                     GGYDPVTQTLLQALGALPRENTSADHMMIFPLVCAALIALWWMWPVNRALPGSEAERP
                     GKIKPIKQVVRPAPALRARQR"
     gene            complement(9618..10253)
                     /locus_tag="VNN25_26845"
     CDS             complement(9618..10253)
                     /locus_tag="VNN25_26845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXH95221.1"
                     /translation="MKALDAVTFTNVYPVKSVLFAEGQAARGAFVMCHGTAKLSISSN
                     DGKTLITKIVAPGEVLGLSGSISGNPYKTTAETLETSQVNFVSRIELLHFIETYHQAS
                     LSAGRTLALECEADVDHIRALELSHSATERLASLILSWCPPDQQPCRGCRFPMLMTHG
                     DLSQLIGSSRETVTRILKMFREKKLISMKGSTLTILNADALEGLAQDERPA"
     gene            10896..12161
                     /locus_tag="VNN25_26850"
     CDS             10896..12161
                     /locus_tag="VNN25_26850"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXH95222.1"
                     /translation="MSQTTGTRRRVARAIRGLMLAMVAGCAAVPRNPAPAAFVGTGRP
                     AGLPAEIRGSGLDEERFAALSPRVIERVRAAATDGTVDVLALSGGGAGGAFGAGALTG
                     LTQRGTRPQFEIVTGVSAGALIAPFAFAGTAWDPQLTAALAGVGTGGILQRRTIDLFF
                     RPSFYRGKPLAAFVNRVISDALIEKVADEAGTGRLLLVATTELDSRSTIIWSMGAIAH
                     ARTAASRKLFREVLIASASIPGIFPPVLIRVETDRGPFDEMHVDGGATVPFFVAPEVA
                     SIVPGLFAGLPGANLYVVMNTQLGGLPDPVPGRLGPIMARSSSVALTYMSRKELQVAA
                     ALAQDHGMKFLLTEIPIDYPFGGPIDFEANKMRMLFEYGQRCAAQGHLWTTPAEALRR
                     SRAAVAGAASRKERLSPENVPCPIDGSRP"
     gene            12579..12932
                     /locus_tag="VNN25_26855"
     CDS             12579..12932
                     /locus_tag="VNN25_26855"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HXH95223.1"
                     /translation="MVSPKERQSFFRDFSRIHDGALVTVTVAAPGAGSHDEIVNQSLR
                     GISEERDEIVVNTGNGGGRPRFARHVRNAGTVLLQQTDEGADAELDIASDDGSLTIVR
                     FRSPALPELLDPGVE"
     gene            12951..13358
                     /locus_tag="VNN25_26860"
     CDS             12951..13358
                     /locus_tag="VNN25_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HXH95224.1"
                     /translation="MKHDEFVGQLQHRAHLGSRGEAERAIRATLETLAGRIPEATAHH
                     LAAQLPPEIAESLLHGTAERLDLDDFFKHIAERERKSVATAAFHARLVLSLITQVVSP
                     GIMLKVRRELPESFGMLFLPERGAGTQPHHAHA"
     gene            13368..13922
                     /locus_tag="VNN25_26865"
     CDS             13368..13922
                     /locus_tag="VNN25_26865"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXH95225.1"
                     /translation="MNAQKLLVIFATHYGQAALIAKRIAGEAIASGAEATVRDVRKAS
                     DADLERCDSLVIVASVQFGRHARSVERFVKRNSDRLGSMHSAFISVSGSAARSETYDE
                     AETIVRKFFSATGWCPDEYQLIGGAVKFKRYNPLLRFVIKRIWASKGLAMDTKRDYDF
                     TDWDAVKRFTKAFVASETPHRAVA"
     gene            14022..>14920
                     /locus_tag="VNN25_26870"
     CDS             14022..>14920
                     /locus_tag="VNN25_26870"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXH95226.1"
                     /translation="MRRINWLIVSLVLSAPLHAQLLVTTDWLQAHRSDKRMVIVEIGD
                     RASYEKQHIAGARFIALSDLVTDRDGIPNELPEIAALERTFASAGIGNRDRIILYSRD
                     VIAAARAFFTLDYLGHGDSTSLLDGGFAKWSAENRPVEASAPLLLAKTTDFQARPRAA
                     TIARLKAMTVLVDCLPVLDGSWAVVDARSPEQFSGAEPGTGIKRGGHIQGALNVPWSE
                     NLTSSATPVFRSRDELEALYSDSGVAANASVVVYCRTGMQASVNYFVLRYLGRDVALY
                     DASYFEWSRSPHPLISTGISTIGG"
BASE COUNT         2779 a         4841 c         4485 g         2815 t
ORIGIN      
        1 atcgactcac tgagccaggg gcggatcgtg acgatcggag gcgatccgat ctgtgcgtcg
       61 cgtccccttc cgcttttcta cagccgccgg cagtttgcac cggcgtggag cgcgcgcgat
      121 cgcgacgacc tgctggccgc cattcgcgat gccaagaacg acggcctcga tccggcggac
      181 taccatctcg cagcaatcga acgaacgtct gatcccgagc agcgcgacct tctgctcacg
      241 gacgcgttct ttctgctcgc ctcgcacttg ctttcgggcc gcgtcgatcc ggaatcgatc
      301 gagccgacct ggtgtctgac gccgcgcggg aacgatctcg tcgctgcgtt ggagaccgcg
      361 ctcgagctgc acgacgtgcg cgcggcgatc gcgcggatgc cgcccgcaca tccggagtac
      421 gccgcacttc gccgctggct tgcgacatac cggcgcattg ctgcggccgg cggatggccg
      481 gatattgacg aaccccggga cgcgaagaaa tctctccggc tcggcgaccg cggcccgatg
      541 gtcgcgcaac tcgcggcgcg cctcgttgcg agcggagatc ttgcgcaggc cagcgacgaa
      601 tttgatgcct ctctcgatca cgcggtgcgg cgattccaat cactgcaggg cctcagcgcc
      661 gatggcgtgg tggggcctcg cacgcgcgcg gagctcaata tcccagcggc cgcgcgtgtg
      721 cggcagatcg agctgaatct cgagcgctgg cgctggctcc cctccgacct cggcgatccg
      781 cacgcgctca tcaacattcc cgcgttctcg ctcgtgttga cggaacacga ccgtaccgtc
      841 atgtcgatgc ggatcgtcgt cggcaaggac taccttcgca cgccgatctt cagcggcaac
      901 atcacgcagg tcgtcctcag ccctgattgg aatgtgcctg acagcatcgc cgagaaggag
      961 ttgtggccga aggaacatcg cactccgggc tacctggccc gtgagcacat tgaagtgctg
     1021 cgcggcggca agctgcggca gacacccggg ccgtggaact cgctcgggct gatcaagttc
     1081 aacattccga acaagtacga cgtgtacctg catgacactc ccgcgaagag tctcttcgag
     1141 gccagcgctc gcgcattcag tcacggctgc atgcgcatcg agaagcctgt ggatctggcc
     1201 gagtggctgt tgcgcgaccg gcccgaatgg acgcgcgacc gcatcgtgca ggagtcgcag
     1261 cgaggcatcg agacgacgat tccggtcacg aagccgcttc cggttcacgt cctctactgg
     1321 accgccttcg tcgacgagtc gggcgagctg cacttcgcgc ccgacgtgta ccaccgcgac
     1381 ggacggctgg acgctgcgat gcgccaggca ccttcccgct tctgaccggc tcgcgagatg
     1441 cggtgactct taaccgcacc gcgaacagtc tgcatcacgc cagcgtgcga cacgcatcat
     1501 cgctcgccaa tgcgcagcga cccatgttct cgcagggacg ttcttccgaa cgccgttccc
     1561 caggaggcac tcatgcgaat ccaggatgtc atgtcgaaga gagtcgtaag catcgccccg
     1621 aaggcgacca tctccgatgc gcgggaaaca cttcgtaccg ttgagatcga tcacctcgtg
     1681 gtcgtttccg gaaaacagat cgtcggcgta atcgcgggca aagatctcgc ccgtgccggc
     1741 gatgaacagc cggtcgagaa cgcgatgaca cgcgacgtcg tcacgatcga gccggaagca
     1801 acgctccggc atgcggccgg agtgatgcgc ggacgcgcca tcggatgtct gcccgttgtc
     1861 gatgacgaac gtctggttgg catcgtgacg acgtcggatc tgctcaccgc gctggcgaaa
     1921 ggggacaccc atacctcgcc gccgagcgag cgcgtcactc tccggaagcg aggcccgagg
     1981 aagcgcgcag cgacaatcta gatcgtggat tctcccgcct caaaatagcc ggaggtcttc
     2041 accgcccatg aacaattttg tgcgattccg tccctcgttc tttgcctgat acagcgcgat
     2101 gtcggcgcgg tggatcagcg catcgaggga gtcgttctgc ggcaacagtt cggcaattcc
     2161 gctgctgatc gtcaccgtga cgacatcctg tttcatgacg atgctctgcg cagaaagatc
     2221 gccgcgcatc ctgtcggaaa ccagggccgc ttcgtgtgcg tgtgtgtgag gaagcaggat
     2281 cacgaattcg tcgccgccgt accgcgcgaa gatgtcggac tcgcgcagat gcgtgcggcc
     2341 caccttcgcg atgcgtttca acaggaggtc gccggcctgg tgcccgagtg tgtcgttggt
     2401 gtgtttgaag ttgtcgatgt cgaacatgac cagcgccagt ccatggtgat agcgcgccgc
     2461 ttccgagacc gcatggaccg caagatcgaa gaagtgccgc cggttggtca ggccggtcag
     2521 atcatcgata cgcccgagcg tccgttcgcg atcgagtgcc gcggccagct cacggctcgt
     2581 cgcttccacg ctttccttgg cccgcatgat cacggcctcg tcgcgttttt gggcgctgat
     2641 gtcctgattg acgatgatgg ctccctcgat cgagccagac tcgccgacga gcgggacggc
     2701 tgaattgaga atgatcttgc gggttccgtc gaagcattca atctcgatct cttcgccgat
     2761 cgatgtctcc ccattgcgaa tggctctggc cgccgcccaa tcttctgcgg ccaccggaag
     2821 tcctgtgctc agccaccacc ctttgtactg accaaactcc tcgggtccaa caaaccgggc
     2881 gcccatccag atctgttgcc cggcggggtt tccgtggacg atccgcccac cggagtcctg
     2941 tatccatacg ccgacgggaa gcgcctccag caccgtacgc agcaggcgct cagcctcacg
     3001 atgcgactgt tcgattcgtt ttcgccgcgt gatgtctgca tgcgaaacca ccgccgccgc
     3061 tcccgattcg ccgcgaagcc gcgtcacctt caacgcgaac catcgcttct ccgttggcga
     3121 gtcgcagggg tactccatcg acacctcgtc cgagtttccg gcgagcagcg agcgcagccc
     3181 atcagtcatt cgttcgagtg gtccgtcttc accgcgcgca agggcatctt cgcagaccga
     3241 aaggtagtcg gtgccgacgt agaaccgttc gtctgcagcg ccgttggcag aagcaaatcg
     3301 gatccacgcg tcgtttacgg ccacgatgac gccgcgtgaa tcaaggaccg cgatgttgtc
     3361 gctcagcgcg ttcagaacgt cgaaggcaag cgttgcggca tgttcgatgt tacgcattga
     3421 tgttcaagtg ggcggcgcca gacgatcagt atcgcaggta tccgtgcgtc tcgacgcaga
     3481 tactcccttc agtcacgaga cgttgtcgcg cgaaaccctc cgtctattcg ttgagaatcg
     3541 caacggtagc cgtcagtacg cagcgaggcg ggtcaattcc tccgtaccgt ggatgatgat
     3601 cgcgtctgcc gtccgctcga tgagaccgct gtggtcaagc tctccgagga ttcgcgtgac
     3661 cgtctcgcgc gtcgtgccga tcgactggcc gatctccgaa tgcgtgatgt gcaatggaac
     3721 gtggattcca tcctctgcga gtatgccgtg ggccgacgct tcttcaagca ggagatgggc
     3781 aaggcgcgcg gccgatgagc ctcccatggc gagaaacttc gtttcccgct gcgcgacctt
     3841 gtaatgctcg ctcagcgcca ccgtcagccg gagacacgcc tcaggatacg ttgtcacgaa
     3901 ccggacgaag gtttctgcag gaatgacgga gagcgcagag gcctccgcgg cgatcgcgga
     3961 gcattggtac ggacggcgtt cgagcatcgc ttctgcaagg ccgagaagcg tgcccggccc
     4021 cgcaaagcgc agcagcagga cctttccctc gctcgaagag gccaccagct tcatgctccc
     4081 ggcgcacacg acgaatacgc tgtggcacgg gtccgcctcg tgaaatgcag cctctccctt
     4141 cgcgaaggtg acgctcgtct tgagcgactc gaagacggcg tgaagctcct ccgagagcga
     4201 acagaatgaa cgcggcgcgg agcatgtggt gcaaggctgt gaaggcatcg gcgaaaccgc
     4261 tggatgagac acgctgcact acctttcgtc tcgggagacg gcgcggccgt cgagtgtctc
     4321 cgcgacgttg atcacgctat cggctggccg cacatggttc ggtgatcgag agcaccgaac
     4381 cgtgtgatgc actgatcgca ctggatggct gacagcgcat caacggagag tctccttctg
     4441 tcagacgggc tgtcgcaccc gaaacctcgc ccattcgtcg agaaccgtaa cgtcagccgg
     4501 cacaggtccg cgtgagtctc ctaccgggta cacgagtcgg agtgagttcg aatcagacgc
     4561 gcattgagag caataagccg ctccgcgcgg atgcctggac atctcacgtc acttgtgacg
     4621 tgcagccgca acgcgtgtgg ggcgcgacac gccttcacgc acgtagcact cacgttcgct
     4681 gcgtcagcat gacctttccc tctgtcatgg atagacccat tgttcaccca gcgcaaaccg
     4741 attgcgcatt gtcagaaagg gagaccgaaa tgagaaaagc aatcaagaca ctcgcgcggc
     4801 tcctgctggt aggcacagca gtcatgattt tgacggcacc actctatgca caagccaccg
     4861 gatcgtcgcc ttacacgatt cccgggatgg gagtccgtac cgccctgcag atcgttacag
     4921 acgaaggcac acgcacgatg cctccggcaa catttcagga ggtcacgccc tgccggtttg
     4981 tcagcacgct tgcggccgac aaatacccgg cgccgtgggg tggcccggcc ttcgcgatcg
     5041 cggaacgccg gatttacacg gtgaggggac cgctgcaggt cgacaccttc gtcaatccct
     5101 gctacggcgt aatccccgac aaggcggttg cgatcgccgt ccgcgtgacg acgtccaatg
     5161 gaagcagcaa tggccaggtc gttgcgttca atccggatct tctgagtccc gatctaatgc
     5221 cggtaggctc gattaacctc aacggcaatt cgactgacga agcaggcgta atgctgggcg
     5281 gaggatgggc gttcggcatg atgacgaaag atgctggcgc tgacctgacg atcgacatcc
     5341 tcgggttctt cttccccgat ccgattgcca ccacccctgg acccgcgggt ccgcagggcg
     5401 aagtcggacc gcaggggccg gccggagcaa ctggtcccac cggcgttact ggtccgaaag
     5461 gtgagaccgg cccgaacggc atcactggcc ccaaaggcga gaccggtccg aacggcgaaa
     5521 ccggcccgaa gggtgaaact ggtccgaagg gcgagactgg cccgaacggc atcactggtc
     5581 ccaaaggcga aaccggtccg aagggcgaga ccggtcccaa cggcatcact ggccccaaag
     5641 gcgaaaccgg accgaacggc gagactggcc ccaagggtga cactggtccg aagggcgaga
     5701 ccggcccgaa cggcatcact ggtcccaagg gtgaaaccgg accgaacggc gagactggcc
     5761 ccaagggcga aaccggcccc gctggcatca ccggtcccaa tggcgagact ggtccgaagg
     5821 gtgacactgg cccgacaggc gaaaccggcc cgaagggtga tactggcccg accggcgaga
     5881 ctggcccgaa gggtgacacc ggtccgcagg gacaaaccgg tccgaagggc gacactggcc
     5941 ccaccggcga aaccggcccg caaggtctgc ccggaattca aggtccgcag ggacaaaagg
     6001 gcaccggagt cgtattcaac tccgctgtca acacaatccc catcagcggc aatatcaccg
     6061 tctatgacgc cactgtgacg acaagcagcg tcatcgtcct cacctactac catggcggag
     6121 gccccgaccc gctctcgctg gacggtcagg gcaacggatg gttcaccgtc tcgggcagca
     6181 agaacaagga attcatgtac gtcgtgctca acaccaacta gagctcgacg ccgcagtaag
     6241 caggaggcac cggcgcacac gcgccggtgc cttttgtcac atggcacatg gaccggcgat
     6301 acgtccttcc cttctccaac ctcctacgct gtccgtatga aacgccactt cccgattctc
     6361 gccctcatcg cagcgtgcat cgcattggct ctcatcgccc tcgcctattc gaatttcttt
     6421 cacaattcct ttcacttcga cgacgaccac gtcatcgtcg aaaacgcatc catcaagtcg
     6481 ctctcccact ggacgcgttt cttcaccgat gcccacacgt tcagcagcca gccgtccaac
     6541 gccacatacc ggcccattgt gacgctcagc ttcgcgctcg actatgcggt tcatggctca
     6601 ctggacccgg ttcccttcca cgtcacccaa ttactactgc tgatcatcgt tggcgtgctc
     6661 ctctcgatcg tctacacgtc gctgttcggg ccgggtcagg agacgctggc cgtcattgcc
     6721 gcgggcatct tttgtcttca cacggccaat acggagacga tgaatctcct ctccagccgg
     6781 agcgagttgc tctccgcgat cggattgctc gcagccttga tcgtattcat ccgttggccg
     6841 gcactgcggc gccgcgggct ctatctcatt ccactggcga tcggcgcact ggcgaaggct
     6901 cctgtcgtga tcttcgccgg aatcgtcatg gcctgggtct ggctagtcga gcggcggcgt
     6961 cccaccgacg cgctcaaggc ggcgctgccg tccttcatca ccggcatcgc gcttctgatc
     7021 gggctcaatg ccatgaacgc gaaggagtgg attgccggag gaggatcgcg catcggctat
     7081 ttgattacgc agccgtatgc ctggctccat tacgcacgtc tggtgattct tcccgcgggt
     7141 ttgtccgccg ataccgatct ccagccgatg gcgcactggt atgacacgaa cgcaatcgcc
     7201 ggctacgcat tcgtagcgct tctctgcctg gccatcgcaa gattcgccag aacacgcgag
     7261 gaggcaccga tcgcgttcag tctggcctgg ttcggaatca ctctactccc gacgtcgagc
     7321 gtgtttccgc tcgctgaagt cgtcaacgag caccgcctct tttttccttt gatgggtgcc
     7381 gcagcggcat tcgtctggac agcggagctc attgtgcggc gttggccaca agcgcagcgc
     7441 ctggcttact cagtcgtcgt catttctgca ttggcgctgg cttacggaac gcacgctcgc
     7501 aatgaagcct ggcacacaga ggagacgctc tggcgcgacg tgacgcagaa gagcccccga
     7561 aatgggcggg gctggatgaa ctacgggctt acacagatgg aggtgggccg gtatatcagc
     7621 gcaaaagagg cgttcgaccg cgccgcgcag ttcacccctt ccttcggaac actggaaatc
     7681 aaccgcggca tcgtcgccgc cgccctcggc gataacgtcg ccgccgagca gcacttccgc
     7741 cgcgctctcg aattgcatcc cgatcgcaat gcgcacttct attacgcacg atggctattg
     7801 cggcgcgggc ggggaacaga ggccgaacct catctggccg aggcggcaaa gattgcgccg
     7861 acgtgggttc cgccgcggcg gctggcgttg caggtagcgg tgggacgcgg tcaggacagc
     7921 aaagcgcgag cgcttgcggc aggcattctt cagatcgatc cgaccgacgc agaagcgctc
     7981 gccgtccagc gcgacggcgc cgatctgcga ggcggcacgt acagagcctg tttcgatcag
     8041 gcgtgggctt ccatgcgcga tgaccagcac gctctggccg cttccggctt ccgcgccgcg
     8101 atccgtttcg agccgaaggc gacggcatac aacaacctcg gctggtctct ggcctcgctg
     8161 ggattccgcg atgacgctgc agtggcgtac caggcggcga tcacggcgga tccgcagttt
     8221 gaacgtgcac gaaacaacct tcgagatctc gatgcctcaa caactcggtg attacaagcg
     8281 tcttcgcaat ctgacgggca cccagcgctg gttgctggcg gcgatcctca ttatcgccgc
     8341 cgcactccgc atcggatgga acaacgttga gacctattcg ccggccgacg agaccgttta
     8401 tgcgaactac acgcgcacgc tcgtcgagcg agggttcttt cgcgggtatc cggagatcgc
     8461 acagacgttc gtgcgtgatc cgcagcgctg gaacttcccg agccctcttc gctgggggta
     8521 tttcggcctg tcgacactgg ccgcgcacat taaaggaagc accgagccgc gcgctctggc
     8581 ctggttgtcg acgatcgccg gcatcctcgt cgttccgctg atctttgtct tctcgcttcg
     8641 tctcttcgac gcgcgcgtcg cgcttctggc ggccgctttc accgccgtct ccggcatcga
     8701 gctggccctc ggcaggcgcg gactgcagga cgaagtgttc tgcctttccg tgctcgccag
     8761 cacgtattgc gtcatggtca tcgtcacgca acgagacgac gagacaccgc ggtggttcat
     8821 cgctctcgca atcgcgagca tgacgatgtc attcgcgatc aaggagtcgt ttctctatct
     8881 gatccccgcc ttcgccgccc ttctccttct ccaccgacgg ccgcgggagc ttcgtctgct
     8941 ccacgtcgcg ctcttcgctc tcccaccgtt tctctatttc gccggattct gcctgctcac
     9001 gcgaagcgtc ggaacgttct ttcgcgtcgg agagctcgtg acgtccgcga tgaaggcgcc
     9061 gtacgtcgtg cagtatcaga gcgggccgcc acaccgccta ttgctcgatt tcttcattac
     9121 gacgccgctg gtgtcgatcc tcgccgcagc cgccatcgtg ttcatcgtgt tgacggcctc
     9181 caaacgcggc agcgagcgct cgattgcgct attcgtcgtc gtcgcggtgg ctgtcttcgc
     9241 gatcgtgcca tcgaagaatc tccgcttcca cgtcatgctc gatccgttct ttcgcctcct
     9301 ggcggcatgg gtaatcgctt cctcggccgt caccgcaaat gcgtgcaagg cctgggcgct
     9361 ggccggcgtg gccgccgcca atgccgcgat tgagatcgag atcttccaca cgatcttcat
     9421 caagcatggt gggtatgacc ccgtgacaca gacgcttctg caagccctcg gcgcgcttcc
     9481 gcgcgagaac accagtgccg atcacatgat gatctttccg ctcgtctgtg ctgcgttgat
     9541 cgcgctctgg tggatgtggc ctgtaaatcg ggcactgccg ggctccgaag cagaacggcc
     9601 cgggaagatc aagcccatca agcaggtcgt tcgtcctgcg ccagcccttc gagcgcgtca
     9661 gcgttgagga tcgtgagcgt cgatcctttc atcgagatca gcttcttttc acggaacatc
     9721 ttcagaatgc gcgttaccgt ttcgcgcgag ctgccgatca actgcgacaa atctccatgc
     9781 gtcatgagca taggaaagcg gcaaccgcgg cagggttgct gatcgggagg gcaccaggaa
     9841 agaatcaggc tggccagccg ctccgtggcg gagtgagaaa gctccagcgc acggatatgg
     9901 tccacgtccg cctcgcactc cagcgccagc gtgcggccgg cgcttagtga cgcctgatgg
     9961 taggtctcaa tgaagtgcaa cagctcgatg cggctgacga aattcacctg ggacgtttcc
    10021 agtgtttcgg cggtggtctt atacggattc cccgagatgg aaccgctcaa cccgaggact
    10081 tctccgggag cgacgatttt ggtgatgagc gtcttgccat cgttcgagga aatactcagc
    10141 ttcgcggtac cgtgacacat cacgaatgca ccgcgtgctg cctgcccctc tgcgaacagg
    10201 accgacttca ccggatagac gttggtgaac gtcacagcgt cgagtgcctt caacggagcc
    10261 ggcgcaagat cgcagaaaaa acctttgcgc cgccacgtgc acttcagaca actatcgaca
    10321 cgctcaaatc cataaccttg cctgcgcact acatttctct ctcttcctaa gacctgacgc
    10381 gtaccgagcg atcactccgc gttcaagcgc cgctcgttag cgccgtcgga tacgcgtaac
    10441 agattttcga cgacgaccgg catacaggca tcatggacgt tcccgcgccg gcagcagtga
    10501 aaccggtcac cggtggacgt gatggtccaa tccgacattg gtgtcatcct gagactgccg
    10561 gagccgcccg cgtgaatcgc gatgtttgcg cagtatgtgc tcggcggcgt aggacgtcga
    10621 aggatctcaa atttcgcaga tcgtgctcgt ccagcgcgag cgatcgtgat caaagacgaa
    10681 cgagatcttc gtattctgag atccttcgac cgtccttcgc cgccaatgcc tgccggggca
    10741 cgagcagcga atcttgcggc ggctcggcgg ctcaggatga cacatcacgt tttgcgcata
    10801 tcgtcaaccg ctttggcggc tcagatgaaa aagcaatcgc ggctacgagt gtccttctcg
    10861 ccccctgtga tgcctccgag tacactccat aacgaatgtc gcagaccacc ggaacgcggc
    10921 gtcgtgtcgc gcgggccata cgcggattga tgctcgccat ggtcgcggga tgtgctgcgg
    10981 tgccgcgaaa tccggcgccg gcggctttcg tgggtacggg ccggcctgcc ggtctgcctg
    11041 cagagatacg aggaagcggt ctcgatgaag agcgcttcgc agccctctcg cctcgggtaa
    11101 tcgaacgcgt tcgcgccgcg gccaccgatg gcacagtcga cgtgctcgcg ctttccggcg
    11161 gcggtgcggg tggtgccttc ggcgccggag cgctgacggg cctgacacaa agagggaccc
    11221 gaccgcagtt cgagatcgtg accggcgtga gtgccggagc actcatcgcc ccgttcgcgt
    11281 tcgcgggaac ggcgtgggat ccgcagttga ccgcggcact ggcgggagtg ggaacgggcg
    11341 gcattctgca gcgccgcacc atcgacctct tcttccgccc cagcttctac cgcgggaagc
    11401 ctttggccgc cttcgtgaac cgcgtgatct cggacgcgct gattgaaaag gtcgcggacg
    11461 aagcaggaac cggccgtctg cttctcgttg cgacgacgga gctggacagc cggtcgacga
    11521 tcatctggag catgggagca atcgcgcacg ccaggaccgc ggcatcgcgc aagctcttcc
    11581 gcgaggtgct catcgcctcg gccagcattc ccgggatctt ccctcccgtt ctgatccgcg
    11641 tcgaaacgga tcggggacca ttcgacgaga tgcacgtcga tggcggagcc accgttccgt
    11701 tcttcgtcgc tccggaagtt gcgtcgatcg ttcccgggct gttcgccggt cttcccggcg
    11761 ccaacctcta cgtcgtgatg aacacgcagc tcggcggatt gccggatcca gtgccggggc
    11821 ggctcggacc aatcatggct cgctcgtcat ccgtcgcgct gacgtacatg tcgcgcaagg
    11881 agcttcaggt tgctgccgcc ctcgctcagg accacggcat gaagttcctc ctgacagaga
    11941 ttccgatcga ctatccgttt ggcggcccga tcgacttcga agcaaacaag atgcgaatgc
    12001 tcttcgaata tggacagcga tgcgctgcgc aagggcacct ctggaccact ccggcagagg
    12061 ccctgcgccg ctcgcgtgcc gcggttgcgg gtgccgcttc gaggaaggaa cgcctttcgc
    12121 cggagaatgt cccctgcccg attgacggga gccgcccata acgtgagcgg gtggggtcac
    12181 accttcggac gtcgttcgct cccgatcatg ctcgcgcaag tttcggtctg gcagtgactt
    12241 acagcaaggg acacaattca cggcactgcg tcatgtgaga ggtcacgaag caggcaagcg
    12301 ctgcgtttgc agcagcttag gcacgaattc gagcctgacc acgcggcgat gccttcctgg
    12361 tatggcggac tcaactcaat gaaactgcgc gagttatgtc tgcgttcgag gagaccggcg
    12421 aagaccgaag gtgcgacccc aaggacgcca gagcgcgcgg cggcgcgctt caaccagtga
    12481 tcgatcgcac tccaatgcgt gcccgtgctc gcagccgccg gacggccgac aacctacctt
    12541 gcagtagacg gatcaggagg ccgcgtatga gaacttgcat ggtttccccc aaggagcggc
    12601 agtcgttctt tcgcgacttc agccgcattc acgacggagc actggtcaca gtcaccgtcg
    12661 ccgcgcccgg agccggttcg cacgacgaaa tcgtcaatca gtcgctccga gggatcagcg
    12721 aagagcggga tgagatcgtc gtcaacaccg gtaatggcgg cggccgcccg cgcttcgcac
    12781 gccacgtcag aaatgccggc accgtgctcc tgcagcagac ggatgaaggt gccgacgccg
    12841 aactcgatat cgcctcggac gacggcagcc tcaccatcgt tcgcttccgc tcacctgcat
    12901 tgcctgaact gctcgaccct ggcgtcgagt gacatcaaac ggaggacccc atgaaacacg
    12961 atgaatttgt cggacagctt caacatcgcg cacacctcgg ctcccgaggc gaagcggaaa
    13021 gagcgatccg agcgacgctc gagacactcg ccggccgtat cccggaagcc accgcgcacc
    13081 atcttgccgc ccagcttccg ccggagatcg ccgagtcact tcttcacggg accgcagaac
    13141 ggctcgatct cgacgatttc ttcaagcaca ttgcggagcg tgagcgcaag agtgtggcca
    13201 cggcagcgtt ccatgcgcgc ctcgtcctgt cgctcatcac gcaggtcgtg tcaccgggca
    13261 tcatgctgaa agttcggcgc gagctgccgg aatcgttcgg catgttgttc cttcctgagc
    13321 gtggcgcagg cacccagccg caccacgcac acgcgtaggg ggcgaccatg aacgcgcaga
    13381 agctgttggt catcttcgcc acccattacg gacaagccgc gctgattgcg aagcggatcg
    13441 ccggcgaagc aatcgcgtcg ggagccgagg cgacagtgcg cgacgtgcgc aaggcgtcgg
    13501 acgccgatct cgagcggtgt gactcgctcg tcatcgtcgc gtcggtccag ttcgggcgtc
    13561 acgcgcgttc ggttgagcgc tttgtgaagc gcaacagcga ccggctcgga tcgatgcact
    13621 ccgcattcat ctccgtcagt ggaagcgccg cacgatccga gacttacgat gaggccgaga
    13681 ccatcgtccg caagttcttc tcggccaccg gctggtgtcc ggacgagtat cagctcatcg
    13741 gcggcgcggt gaagttcaag cgttacaacc ccctgcttcg cttcgtgatc aaacgcatct
    13801 gggcaagcaa gggtctggcg atggacacga agcgcgacta cgacttcacc gactgggacg
    13861 ccgtgaagcg atttacaaaa gcgttcgtcg cgagcgaaac gccgcatcga gccgtcgcct
    13921 gaaggcgcga tgcggcccgt cgctcgcttg cgtggcgatg gtcactccga cccgtgacca
    13981 gacgcgcggc gctttcgcgg aagagcgcct acgttgattg catgagaaga atcaactggc
    14041 tcatcgtctc tctcgtcttg tccgctcccc tgcacgcgca actgctcgtc acgacggatt
    14101 ggctgcaggc acaccgctcc gacaaacgga tggtcatcgt cgaaatcggc gatcgcgctt
    14161 catacgagaa gcagcacatc gccggcgcgc ggttcatcgc gttgagcgac ctcgtgaccg
    14221 accgcgacgg cattcccaac gagctgccgg agattgcggc actcgaacgg acattcgcct
    14281 ccgccggcat cggaaatcgc gaccggatca ttctctacag ccgcgatgtc atcgcagccg
    14341 cgcgcgcctt cttcactctc gattacctcg ggcacggaga ctcgacgtcg ctcctcgacg
    14401 gcggcttcgc caagtggagc gccgagaatc gcccggtcga agccagcgcg ccactgctgc
    14461 tcgcgaagac gactgacttt caggcacgtc cgcgtgcggc aaccatcgcc cgcctgaagg
    14521 ccatgacagt cctggtcgat tgcctgccgg tacttgacgg ctcctgggct gtcgtcgatg
    14581 cacggtcgcc ggagcagttc agcggcgccg agccgggtac cggcatcaag cgcggcggtc
    14641 acatccaggg cgcgttgaac gtgccatggt ctgaaaacct caccagttcc gcgacgcccg
    14701 tctttcgcag ccgcgacgag ctcgaggcgt tgtacagcga ctccggcgtc gctgcgaatg
    14761 cgtccgtcgt cgtctactgc cgcaccggca tgcaagcctc ggtcaattac ttcgtgcttc
    14821 gctacctcgg ccgcgacgtc gccctctacg acgcctcgta cttcgaatgg agccgcagcc
    14881 cgcatcctct gataagcacc ggcatctcga ccatcggagg
//
LOCUS       DATLVW010000906         3708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_164200, whole genome shotgun sequence.
ACCESSION   DATLVW010000906 DATLVW010000000
VERSION     DATLVW010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164200"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VNN25_26875"
     CDS             <1..267
                     /locus_tag="VNN25_26875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95227.1"
                     /translation="LDDSNGHGAPYQTVGDDGRRHATTAYTRRQHDRCNNNDSSNLND
                     PAGDNFVRIQYPDPVRPASNHHARTETQTGAARRTRTGRRNSGA"
     gene            290..655
                     /locus_tag="VNN25_26880"
     CDS             290..655
                     /locus_tag="VNN25_26880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95228.1"
                     /translation="MLTYREKGDGHANDAALASLHQQIAGTTRVYLSGGDAEMIKQLS
                     DALRGRGVSLAEGAPIEIRFTGSVNSHGFGRKSRSGNATIKKNGRPIFQYVMPREDYR
                     VGDSPAEAFARILADALAR"
     gene            699..1097
                     /locus_tag="VNN25_26885"
     CDS             699..1097
                     /locus_tag="VNN25_26885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH95229.1"
                     /translation="MTYPAIKPRLTSFAPQFLVDDLERSIAYYRKIGFTFGEVWGGFY
                     AIGHLDGLELHLKEPPRNLVERQHRRDNEHLDAAAGVDGIESFYEQCVANGVAIHKSL
                     APTAWGTKDFYIEDPDGHIISFGGRPAPAT"
     gene            1373..1654
                     /locus_tag="VNN25_26890"
     CDS             1373..1654
                     /locus_tag="VNN25_26890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95230.1"
                     /translation="MQQIASMFSLQPDDIHHVVAHDDFWLRHVQRSPGPSDGFYLLAN
                     DPGYTTYEQERGAQFEKQRWDTFEQARAAYAAKLVRDLEVLRRNAAAGR"
     gene            complement(1889..2134)
                     /locus_tag="VNN25_26895"
     CDS             complement(1889..2134)
                     /locus_tag="VNN25_26895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95231.1"
                     /translation="MTPAPVAKSIASGTLRLRAERDANGRGRVYLIRITATDAGKALG
                     KRGRKSKSKPAPKPATAVSTESSPVTSPSSTNHPKSK"
     gene            2243..2902
                     /locus_tag="VNN25_26900"
     CDS             2243..2902
                     /locus_tag="VNN25_26900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95232.1"
                     /translation="MFAIISAPLGVAMSPLADAVRPGNAKKTASSRPATLELPEWVGQ
                     RFMLLAMPQKSQGYGYQSFEPNLPYAQWVGKILTVTAVDSGSPRTVTFKAEDGTPLTA
                     TAYSDKINYIAPMRDLTYARDTWLGRFLWLRGPDLLTFDAATEKFGSIHVGKGARIKV
                     TGVAAGMLDYEPVHLELVADDGRRGFLDIQLTGTNVSTALRGTDTFDDVFAETDPHSS
                     H"
     gene            2987..3520
                     /locus_tag="VNN25_26905"
     CDS             2987..3520
                     /locus_tag="VNN25_26905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95233.1"
                     /translation="MHRLRFALVVLLAVFATGATAATGVKSRWWERFYTRHVSSEGYE
                     ATFYLPDGWSVDPDQRVLVPSDENLAACRIRFDIYRGVPFRQGLAAGLRHDKESTGQA
                     YRSELARVSGAEAVWVWYTDRQGNGVGRTYIDRSTDQSARYVVWQFDIPRDAGANDCD
                     RDFEAIVRSSDVNPVEK"
     gene            3517..>3708
                     /locus_tag="VNN25_26910"
     CDS             3517..>3708
                     /locus_tag="VNN25_26910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95234.1"
                     /translation="MKTLFEPADRESIETRLLALRADSARGWGKMNASQMVTHCARAL
                     ETGTGDRPMKQAFIGKILMP"
BASE COUNT          735 a         1167 c         1115 g          691 t
ORIGIN      
        1 ctcgacgaca gcaacggcca cggcgccccc taccaaaccg tcggcgacga cggtagacgt
       61 cacgccacca cagcctatac tcgtcgacaa cacgaccgct gcaacaacaa cgactccagt
      121 aacctcaacg atcccgccgg cgacaacttc gtccgcatcc agtaccccga ccccgttcgt
      181 cccgcctcca accaccacgc acgcaccgaa acccaaaccg gtgccgcaag aagaacccgc
      241 accggtcgaa gaaacagcgg agcctgagcc ggaacccgca ccagcgagcg tgctgaccta
      301 tcgtgagaaa ggcgacggcc acgctaacga cgcggcgctg gcctcactgc atcaacagat
      361 cgccggcacc acgcgcgtct atctctccgg cggcgacgcg gagatgatca aacaactctc
      421 cgacgccctc cgcggccgcg gtgtcagcct ggccgagggc gccccgatcg agatccgctt
      481 caccggctcc gtcaactccc acggcttcgg ccgcaagagc cgctccggca acgccacaat
      541 caaaaagaac ggccgcccga tcttccagta cgtaatgccc cgcgaggatt accgcgtagg
      601 cgacagcccg gcagaggcgt tcgcgagaat tttggcggat gcgttggcaa ggtagtacca
      661 gagcagaatt gcggttcgcc cgctgaaagg agatttcagt gacctaccct gcgatcaaac
      721 cgcgactcac atccttcgct ccgcagttcc ttgtcgacga tctcgagcgc tcgatcgcgt
      781 actaccgaaa gataggcttc actttcggcg aggtgtgggg tggtttctac gccatcggtc
      841 atctcgacgg gctggaactt cacctgaagg agccgcccag gaacctggtg gagcgacagc
      901 accgccgcga caacgagcat ctcgacgccg cggcgggcgt cgacggcatc gaatccttct
      961 atgagcagtg tgtcgcgaac ggtgtggcga tccacaagtc actcgcgcca accgcctggg
     1021 gcacgaagga cttctacatt gaggatcccg acggccacat catctccttc ggcggccggc
     1081 ccgctccggc tacgtagatg gtcttgggaa ctggggtcag gtctgaagta tgaagtgacg
     1141 gtgccgaatt gtcgttagaa gcaggttctg tgatccgtca cttcatactt cagacctgac
     1201 ccccattccc gcacaaggcg gtacctgttc gcgtgcggct ccaagccgat gagcttgggt
     1261 ccgtttgggc gtccaaatga caagaccaac cgtcagtgac caggttaaag cgctcgcagc
     1321 gggcctcggg tactccctgg cattctccca ttttcatacc gaccgtccat cagtgcagca
     1381 gatcgcaagc atgttctcgc tacagcccga cgacattcat cacgtggtcg ctcacgacga
     1441 cttctggctt cggcacgttc aacgttcgcc aggaccctcc gatggcttct accttctcgc
     1501 gaacgatccc ggctacacca catatgagca ggaaagaggc gctcagttcg agaaacaacg
     1561 gtgggatacc ttcgaacagg cgagggcagc ctatgccgcc aagcttgtcc gagacctcga
     1621 ggtgctccgc cgaaacgccg ccgccggtcg gtgaacgccc aaccagactt ttgcagcggg
     1681 acgctgtctc gcaggcagcc gcgtcaggcg cacgttatga tccggcgtgc gccctgcggg
     1741 ggtttccgtc aaagctaacg atttgcgcac aggaaccacc gtcgggcggg gacgcccgac
     1801 gggagcagac aggaatgtct gcactacggt ctaaatgcgc aggcgcgcct tcggacgcgc
     1861 ccgcgcggga cttactgcac gagagcggtt acttgctctt tggatggttc gtcgatgacg
     1921 gtgatgtcac cggcgaggac tccgtggaaa ccgcggttgc cggtttcggg gccggcttgg
     1981 acttcgactt acgtccgcgc ttgccgaggg cttttcccgc gtcggttgcc gtgatgcgaa
     2041 tgaggtagac gcgtcccctg ccgtttgcat cgcgttcggc gcgcaggcgt agggttccgg
     2101 aagcgatcga cttcgccaca ggtgctggag tcaaggatgc tggtacgcgg cgctggcggg
     2161 ctccccaaac tccggtgcag tagaatcatt cgcgcgagga agtggggagg tcccaatgcg
     2221 aagcctgcgt gcggtaatgg tcatgttcgc aatcatctct gctcccctcg gtgttgcgat
     2281 gtctccgctt gcagatgctg tgcgcccggg aaacgcgaag aaaacggcgt cctcacgccc
     2341 cgcaacactg gagttgcccg agtgggtagg gcagcgtttc atgctcctcg cgatgccgca
     2401 aaaatctcaa ggctacgggt atcaatcatt cgagcccaat ttgccctacg cgcaatgggt
     2461 cggcaagatc cttactgtta ccgcggtcga ttccggaagt cctcgtaccg taacgtttaa
     2521 ggctgaggac ggaacacccc ttacagcgac ggcctacagc gacaagatca actacattgc
     2581 tccgatgcgc gatcttacct acgctcggga cacgtggctt ggccgattcc tatggctgcg
     2641 cggccctgac ctgctgacgt tcgatgctgc gacggagaaa ttcggaagta tccacgttgg
     2701 caagggggca cgcatcaaag tcacaggcgt cgcggcgggc atgctcgatt acgagccagt
     2761 gcatctcgag ctcgtggcgg acgacgggcg ccggggcttt ctggatattc agctcacggg
     2821 aacgaacgtg agcacggcgc tccgaggcac cgataccttc gatgatgtct ttgccgagac
     2881 ggacccgcac agcagccatt gagattcaag ccatccgcgt ctccgcgtga aacggttctc
     2941 tcgtgctacc tcgcaacgcg ttatgatttc gtttgcgagg tgacgcatgc atcgtctacg
     3001 gttcgctctc gtagtccttt tggcggtatt cgccaccggt gcgacggcgg cgaccggagt
     3061 caagagtcgg tggtgggagc gcttctacac gcggcacgtt tcctccgagg gatacgaggc
     3121 aacgttctat cttcctgacg gttggtcggt cgatccggat cagcgggtgc tcgttccctc
     3181 ggatgagaat ctcgccgctt gccggatccg attcgacatc tatcggggag ttccattccg
     3241 gcaagggctt gctgcgggtc tccggcacga caaagaatcg accggccaag cctatcgatc
     3301 ggagctcgcg cgggtgagcg gcgccgaggc ggtgtgggtt tggtacaccg accgccaggg
     3361 gaacggcgtc ggcaggacgt atatcgaccg gtcgaccgat cagagcgccc ggtatgtcgt
     3421 ctggcagttc gacattcctc gtgacgcggg tgccaacgac tgtgaccgcg acttcgaagc
     3481 aatcgtccgt tcgtctgacg tcaaccctgt ggagaaatga aaaccctctt cgaaccagcc
     3541 gaccgcgaat cgattgagac gcggcttctc gcgctgcggg cggacagcgc gcgcggatgg
     3601 gggaagatga atgcctcgca gatggtcaca cactgtgcgc gcgctctcga gacgggtacg
     3661 ggcgaccggc cgatgaaaca ggcgttcatc ggcaagatct tgatgccg
//
LOCUS       DATLVW010000907        22051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_37640, whole genome shotgun sequence.
ACCESSION   DATLVW010000907 DATLVW010000000
VERSION     DATLVW010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22051
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37640"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1052
                     /locus_tag="VNN25_26915"
     CDS             39..1052
                     /locus_tag="VNN25_26915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95235.1"
                     /translation="MTPPVVSPAPPDKRSAIERNDPALASYLGACDEAARRRELELIL
                     LRVQPVVTAVLNRFERREFHPRAEDRDDMTSVVTLRFIDKMRRLTAGGTTPIENLDGY
                     VAVLAQNAAADVLRERYPEMRRLRRRVRRLISSDERLAIWYSGNVTVCGKREWQGREA
                     APLAIAPDSASAAMCDHVNAAESLLAILTYTGHPVLFDELISLVAKLWDIADDMAIDV
                     AGTDPADGQASPETEYESRRFLEAMWEEVKALRTAQRAALLLNLRDIDGSNALSLLVI
                     LGIASFDDIAAALEITPQRLGDLWKGLPLEDLAIASMYGITRQRVINLRRAARERLFR
                     RLR"
     gene            1091..2005
                     /locus_tag="VNN25_26920"
     CDS             1091..2005
                     /locus_tag="VNN25_26920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95236.1"
                     /translation="MNKHLTVEELVRFRTGDLSAEETVRVGQHLAKCKECAAAARQSQ
                     DAAHVAQGFRDALRDGERTAGRRWKRMAAIAAAVLIGIVSVIAYRMTSVTRPAAPPSA
                     AHTARIDYGRSDWNELVNQALANGRVAIPDLTELSAAGGTVRSEEGAAQKVDPEGVVV
                     ESDRPRFSWPAVLKAATYEVVVYRGEREVLHSGKLRVTTYVPERSLERGAVYQWQVLV
                     TEEGGAVRILPTGPAAPALIRILPAPDAVALTDARRHFPGDALLAGTLKARYGLLDDA
                     RRDLTAAAQQHPGDAPVARLRDTVTNHR"
     gene            2012..4999
                     /locus_tag="VNN25_26925"
     CDS             2012..4999
                     /locus_tag="VNN25_26925"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH95237.1"
                     /translation="MILAMVLAAVSLNGQAATHRDIALAGIGALRRALANGARDREIR
                     SIRHRFPCIDVADVRVGPVEIDGKSAAVSLDVMAQEAGFPSHWLVRLRRGQSGWRAVR
                     FDVEESLLADRVAAAAGLDAGRRLLAEHPALITPELSRRLSERSDEIEEQSDGRHAEE
                     VAQLALEVAEDWDDVAGQARALWAAGRATNLLDRLDVAIRDYSEGYALAARVNDTALM
                     AANLVGLGTEYLRKGELNRTKELEEGLRLARLAHDDRIAWMATHRLANVDRVAGRYDA
                     AIRKYEEAVILAARAGDASGQAAATSDIGITYDYMNSRALAYVYIRRGVALYRRTANT
                     IGLIRNIRNMADMEAWDERYADAEKHLREVEALLRQHPNDRTSAFVAATRSTLAIARH
                     DMAGAEKACEQARALASRTDLPDLVTNMTEQLAGVRVRQKRYGEAASLAREAIERSRT
                     GTPDFDIYWQAQLELGRALAGEGKLNEARAAFEDSIGAIEGTLAEVPQGRDDQQAFFA
                     DKVFPYYEMFRTFIADRPEEAIPWVERARARTLLEFLAAKKRRASSRSLTTEEQTREA
                     AIERGIADLNIRISEERARSQRNDAQIEAIERDLAAKRLEFSVFVRRLYEAHPDLAMA
                     RGEVPAATVAEIRRTIPADGAVLEYICLPERCWVIVLTRDGPPRIDALQADRGTLHRL
                     ILEFSAELAARGQGFRAKGRRLYDLLIAPADSVVRNKRILCIIPDVPMGNLPFQALID
                     RYDKYLMERSAVFYAPSAAFLVWRATHAPAPPANDAANLLAFGNPRIAAETTRAAHAR
                     TRSESLASLPEAEEEVRALERMYGAHATVRVGAAATEADFKKEAPHFRLLHLATHGMY
                     DDADPMYSHLVLARRAGDGEDGLLEAREVADLDLHADLVVLSACETGRGGERAGEGMI
                     GLSWALLAAGSETAILTDFKVDSAASRDLMIAFHHRLLQQGGKRMSGRVAVEALRDAE
                     IAALKSRRSHPFYWASFFAAGGW"
     gene            5187..6941
                     /locus_tag="VNN25_26930"
     CDS             5187..6941
                     /locus_tag="VNN25_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95238.1"
                     /translation="MTLRRLSLVLLLAVTAFAYRAHACETDASNYSTNHFNPNFRNNT
                     NQDLGDYGLIFQDAIDRNCSGGSWSPQQGAYRALLNKINSTFTYQDCGSSPGMHNTPF
                     VGCSGPFQGWLEGVNVSLIFSSALLIGGHGDLTTELDQALMRVRDSYNGATSPYCGFT
                     ARPGFPTQWSPGDTCMDDYSIAASAFAWIALYERMRGRDATAYVSQARGRIAQTFRTY
                     DSVCIFDPTVAISSTRGQCNIDTGSMTESQQAAFLKQELFPNPNLGGHTLADIIALNR
                     GENIVYGAGLMTAVSSAFVALDEGGFFPSLTDSQQVIAAAFMDEAQRKTATNGANFGF
                     DCAIFDAAANRSDTQGCADGNATPMIYDLNSKAANLYYPDYGRSSFYDKYVSYKPADP
                     GTTIYDHANGGYIPIFGPFQFNTFSVAPFTKDANDTNENWGRQVFYHYLGFGWNTIDN
                     TRYQANTDHTSGDARPRLWGYLDDHDPIGYLDGIDANGVAIGWTCDNDLPTWTNAVDF
                     YVNGTSGQRITRAFANVASEPAVNSLCGGGYSHRFSVQMPSWTKGQLIYAYGLDATWR
                     GITLLNGWTCAQHPACTW"
     gene            7056..7442
                     /locus_tag="VNN25_26935"
     CDS             7056..7442
                     /locus_tag="VNN25_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95239.1"
                     /translation="MKASKKVFVAMALAVVICTWGHPVSAEPDQQRVYYNCVDAYNDP
                     LLTCFGDDSCIDEYGDVVNCADISGSQTCQQYLVTCESACSSQESADMNACARIQDPI
                     DQANCMAVAYTTAQACDNACLGICLN"
     gene            7476..8177
                     /locus_tag="VNN25_26940"
     CDS             7476..8177
                     /locus_tag="VNN25_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95240.1"
                     /translation="MPRCRTSLALAIVLAPLLVQSLAADAGAQETEKRFHYRFLPVPA
                     PLTDDPRIVRATAIVKSSDFKLIVDFGRRSWLIYDAQADRAHCTNHERIEYGPSDPSH
                     PPLLVDGPIADCVAGTMAPALTSVRAGTRTFTLGSEQWKAGGPAALDEESGPEMANAI
                     KSLIPVLAALNPYVRILCNRMAPLYGLRCDESQPAEAAYTVSPIPVDCVFDARHGAPC
                     VRRFMIAPAGLINGH"
     gene            complement(8174..8302)
                     /locus_tag="VNN25_26945"
     CDS             complement(8174..8302)
                     /locus_tag="VNN25_26945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95241.1"
                     /translation="MLGRCHPHFGGITDATLEALAARAGYRIETRWADARGWFTDF"
     gene            complement(8311..11361)
                     /locus_tag="VNN25_26950"
     CDS             complement(8311..11361)
                     /locus_tag="VNN25_26950"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH95242.1"
                     /translation="MRWVIAAALMAFCLPVLGASESRQALTEDFARALVNLDMRSLDA
                     IATHPIRYVAAWNSVETLVTSSRCIELRSQPRVDMIGSGMSLQGRVTIDAVATGARTG
                     RLRGVPKSWILTFARVQGHWRISEVETYEDLLAKRLATFASSDSYDGELRAGGYDSAA
                     LLTALTKQLQQYPAIPMRRRIADAALMVATSDAELARAWCINARAVLADAQQQSRLIE
                     RCRAAAVASQDTDVIAEASLAGASRSANSVRSRILSLENSVSMLHLVDDQSVPLNALA
                     TLADLYHAGGDERQALMLARRLQELAAVCRIGRAEFDAHTTMLGIYYEQDYWDLAIQE
                     AQAAVHFADWEGDKITLLSAHSNLGACHEMSHHFDASLREYERAAELAGDLGGPIAAI
                     NSANLAGSLMNHNRLQEASEWASIAVERARATHSTPAQIEALIARSRVAYAQSRFEAS
                     GDDAAEAASLARHDHNASELEALVAEADALECVGKPDQSLPILDQAIELIETRRSSLS
                     GEPDSQARFFSDHRTAHLSAIRILVDGGRLAEALSYAERATARVLLGSIQDIGSDPML
                     SLSEEQRKTLATLNQRIQHLNGMLIHGAEEPSSLAAKLNDARNELSRFRVARTIEHSG
                     RGVDKLPDLGVMDMDIESDTVALEYVVEQDRTTVFVAYKEHDGLHVEAHQIEISQQSL
                     NEKVDRFLRSLASRDLEYQRDADDLYRLLIAPVSERIAPYRVLCIVPNGSLWRLPFHV
                     LAPGSGEPLLLTHSIYYAPSLGYVHAAHVRARNRNHDQGHDLMAFGNPIPSGAPPANA
                     RGFTAGDLRPLPDAAKEAIGIAALYGTASSRVYTGTSARSSVFRTEAPKYRILHVGTH
                     ALLDDHAPMNSALVLMSDPTTDDDGILDAREIASMSLNADLAVLAACDTAMGRVDEGE
                     GMLGLSWSFMIAGCTTTVVSQWSVDSRATAILMVELHRRLRNGEAVADALRHAALTLR
                     SRPQYAHPYYWAPFIVSGDGTERLTPAAELNPNQALRAASASPK"
     gene            11516..12556
                     /locus_tag="VNN25_26955"
     CDS             11516..12556
                     /locus_tag="VNN25_26955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH95243.1"
                     /translation="MAKLVAPTREQFDQRQIIALNDPLLRPLIDGPDERSRQREIERL
                     VVSIARPIIDRVLVRHFARMIMISGEDADDVVATVMVRLMGKLRLVTGSIDAAIRNFD
                     DYVSEVAHHAARDIVRTRFPARTRLENRLRYVLTRDSRLALWSVSGSSVAGLAAWTDQ
                     AFVMTADELDAVNFGGIVLDPSRPADALAALFKTIRRPVRFSNLVSRMASLWHIHDEP
                     DLNTIESIIDERPSPLAALQSRQLMNILWDEIVQLPRNQCAALLLNLRDSESLDALAL
                     LPLSGVATTSQIADAIGIGVDQLMSLWKSLPLEDRAIGELLNVTRQQVINLRKSARER
                     LRRRVAKRTRQP"
     gene            12553..13632
                     /locus_tag="VNN25_26960"
     CDS             12553..13632
                     /locus_tag="VNN25_26960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95244.1"
                     /translation="MKTHASFDDLLAVRGRELPDRELRMILDHVAGCPKCSALATRVL
                     SLDNASIGLTEQVLGVDEHLFVDEELYPYVEGMLQSDALVRAERHVASCSTCQAEVED
                     LRAARRSLSRSPDAGRWQFMAAAAAALVLLVSIAVVFSTSRRHFPSTSVRPRAITRAT
                     PANDRDAHGSAAFDGEALDPPPLYLSLLSGPGQTRGFDSPRSSARALHPVGEIVESSR
                     PLFSWTSVPDAVYNVSVYRGLRPVASSGELHSTSWTTAEDLPRGVTYTWQVRVRSHGN
                     SVVIPLPTQPMARFAVLSENAESQLEEARRNRPDDHRSLGVLYARLGMLEDARSELRK
                     AAADPAQRDRVTPLLESLDRWPTSE"
     gene            complement(14266..16713)
                     /gene="leuS"
                     /locus_tag="VNN25_26965"
     CDS             complement(14266..16713)
                     /gene="leuS"
                     /locus_tag="VNN25_26965"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXH95245.1"
                     /translation="MNEPTPNDYKHDEIEAKWQQRWAESGIDRSDIDRTRPKHYALTM
                     LPYPSGDLHMGHWYSMAPPDARARFMRMRGYNVLFPMGFDAFGLPAENAAIKNNIHPK
                     EWTYRNIERMRRQLRSMGAMFDWRREMVSADPKYYRWTQWLFLKLFHGDLAYRKSAAV
                     DWCPSCNTTLAREQVWGDDRHCERCKTPVTRKELAQWFFRVTRYVEELLDFSQIDWPE
                     RVRTLQKNWIGRSTGSWIAFAIDAGAKLEVFTTRPDTLWGVTFLTIAPEHELARKWGE
                     ESPEVAQYLDQTARQSDIQREAADREKTGVFTGHYAISPVTSERIPIWVADYVLASYG
                     TGAVMGVPAHDERDFAFARKFGLEIRTVIEPEDGDRLPEPLDAAYTEHGRMINSGPLS
                     GVSADRAINEVIEHLEALDAGRRGVQYRLRDWLVSRQRYWGSPIPIIYCPACGIVPVP
                     EDQLPVTLPDDVEWRPTGESPLKLHPTWRHTTCPRCGGPAERETDTLDTFLCSSWYHL
                     RYLSPDCDDWPFDRDEYDYWMPVDTYTGGIEHATMHLIYTRFFHKALRDAGVTREPEP
                     MLQLRNQGIILGEDSEKMSKSQGNVVAPDELLVRYGADTMRAHLMFFARWDQGGPWSS
                     EGISGTARWLRRVWMSFVDEQDPAGTNDEATRRLRRKLHQTIRSVTHDFESFEFNTII
                     AALMTLHRELTLALNEGCSPEAWREARETYLRLLAPVAPHIADELWERIGGTESVHAQ
                     SWPAWSEGLVAEDEVTVAVQVNGKLRAKLTVSADSTDDQLRQLAFAEPNVAKYVQGAN
                     LERVIIVPRKLVSIVVN"
     gene            complement(16782..18407)
                     /locus_tag="VNN25_26970"
     CDS             complement(16782..18407)
                     /locus_tag="VNN25_26970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95246.1"
                     /translation="MASLSINLIPAEILSRFNADQIVLICNEQLEGKVFGTTTFDPSS
                     LQEIDGLESLSELRQREKVYSITATIMQLTPLMIDKAAREFEHRFRIISELDALLQHE
                     EKVYVVYAVTHFNHKPFEELTIFIGGDCYRTGSEIRDLETVLSRTKNLAQAMIRKAVL
                     VFPDILTLHGGKKGEWVIVDRAGRKIDGISEEAIVALGTLIIPKGIAFLNGYKEKVAI
                     EKGIYRSFPSRNFMRPDTASPDVLSGPNWKAMCDVWAKRGIDLSLVTCLPEPRGGPRV
                     TSAYPTGYGVVATALKLVSYRFEDRDRAGIQFLLEALGGVGQATVEALIKEKGISPNQ
                     ITAFDKSAEVCKQVSDKYGIKARAMTHEDFYKGLDNSQSYDVWINNGEGDNTRPEHVQ
                     KLLDCGVRLFCGAANNFLKLRDSEESSKENLKIESLQKIFHAGGWAWPDEAASGGGWT
                     LAVIDVLTRAKGEQSNAPGVKEQILKTIISRNEKLVDDVVLGVTNGSGANGETVWKRV
                     EQLINARVQSTLETSFPVGEILEKADVTQWSLT"
     gene            18708..19163
                     /locus_tag="VNN25_26975"
     CDS             18708..19163
                     /locus_tag="VNN25_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXH95247.1"
                     /translation="MSFEKQLADQGYTLAPLNLDAGRLLHATRSGNLVYTSGQVPVLD
                     DRSIKGKVGSDLTIEEAQEAARLCTINCLRAVQSLAGSIDLIARIVKVFGMVNVAPGF
                     DQTPSVIDGCSDLLISVFGEAGKHARSAVGMTIPLNFAVEIEMIVELRA"
     gene            complement(19222..21591)
                     /locus_tag="VNN25_26980"
     CDS             complement(19222..21591)
                     /locus_tag="VNN25_26980"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH95248.1"
                     /translation="MIVGGGIAGLYAAYRLRQVWESGDAKTRKTVKDRLNVKGKKLRV
                     AILEENPLELGGRVRAVKLPFPGGAVTAEVGPMRFTTRQKLVRRLLNDLKIGTMPFKG
                     DGFDTSYFLRGKHFRDADVKQGKSPYSLQNTSDRPEQNKHLEELVQMVFDSTVAELSI
                     DSDSYFDDGSVFAKKHYIAHPTRRALTDILKTESDRKAVARAKRQLDTDYLSTLRALE
                     KLRDKALRETLTHREWLEIQDHCLLRGKIHLRDIGLWNLLHHYLSPDGAKLVEDGFGY
                     ESVLGNWNLSDAIPWFIDDFSLGQSYEAVEKGFGDLIVRLKAALTDSEELKKHPTFTC
                     EIYLNTRVREIGFDLGADIYSVLATTTALRYEHQNDDDRVAASEESDAVKKAKELTWW
                     QFSARAVILAIPKEPLSKLHVDDLLSDWPSDDGKPTKELNRVHKLNRVHKRKWLGDLD
                     TVRAHRLAKLVQAFRTPWWRSPERAKGAGELIITDLPLRQIYYWDQEWLEKRGRYEFY
                     VKGNVTTDAPVRQPDIGGIIVAYLDGHYVSYWRFITTVQRLNARLDAYREDEDGSIEP
                     RETDEEKIIKRIKCAETAEEKRIKCFGDRIWGWSDRPEVSPRRWEDDSKEGNVARMRE
                     FYGRLKEEEPLKPWTDEERVRYLYFRKHGLFARASDKMTHILRDLHGSLKNGHKVTPE
                     PPVVGAYMFWDNFGDEPIPEAGWHTWEPGANSELTMEEMAQPFPDGHGNQGKHRVFVC
                     GEAYSSEQGWIEGALKSVERLIDKLDVRLAGDVCKHVGVCKKGHVHPEP"
     gene            complement(21716..>22051)
                     /locus_tag="VNN25_26985"
     CDS             complement(21716..>22051)
                     /locus_tag="VNN25_26985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95249.1"
                     /translation="VFPSRLNDRMTYQASVFTLHGGKKYVPSLRRHYLGNTIPEPISI
                     AELDDDEQAPLIKRFSIPKCAKKPILEELFKLGIHEGTLFPEVDRQKSYLKTLWWFPD
                     KTSTEGDGA"
BASE COUNT         4102 a         6766 c         6691 g         4492 t
ORIGIN      
        1 aatgttccgt tcccggataa gatgaccatt cgactttcgt gactcctccc gttgtctccc
       61 ccgcgccgcc cgacaagcgt tcggccatcg aacggaacga cccggcgctt gcgtcctacc
      121 tcggtgcctg tgatgaagcg gcgcgacggc gagagctcga attgattctg cttcgcgtcc
      181 agccggtcgt cacggcggtt ctcaaccgtt tcgagcgccg ggagttccat cctcgggccg
      241 aagatcgcga cgacatgacg agtgtcgtca cgctccgctt cattgacaag atgcggcgtc
      301 tcactgccgg cggcacgacg ccgatcgaaa acctcgacgg ttacgtcgcc gtgctggcgc
      361 agaacgcggc cgcggatgtt ctccgggaac gctatcctga aatgcggcgg ctgcgccgcc
      421 gcgtccgccg gctcatcagc agcgatgagc ggctcgccat ctggtattcc ggaaacgtca
      481 cggtctgcgg caaacgtgag tggcagggac gcgaagctgc cccgctcgca atcgcgcccg
      541 acagcgcgag cgccgcaatg tgcgatcacg tgaatgcggc ggaatcgctc ctggcgatcc
      601 ttacctatac gggtcatccg gttctcttcg acgagctgat ctcactggtg gcgaagttgt
      661 gggatatcgc ggatgacatg gccatcgacg ttgccgggac cgaccccgcg gacggtcagg
      721 cctcgccgga aaccgagtat gagagtcgcc ggtttctcga ggccatgtgg gaagaggtga
      781 aggcgctgcg taccgcgcaa cgggcggcgc tccttctcaa tctgcgcgac atcgacggct
      841 cgaacgcgct gtcgctgctc gtgattctgg gaatcgcatc gtttgacgac attgccgctg
      901 cgctcgagat cacgccacag cggctcggcg atctgtggaa gggacttccc ttggaggatc
      961 tggccatcgc gtcgatgtat ggcatcacac gccagcgggt catcaacctc cgtcgcgccg
     1021 cgcgcgaacg gctttttcgt cgtttgcgat agggcgtacc aatatgagtg gcctacggac
     1081 gtcagaatca ttgaataagc acttgaccgt cgaggaattg gttcgcttcc gcactggtga
     1141 cctgtcggcc gaggagacgg tacgggtggg acagcatctg gcgaaatgca aggagtgcgc
     1201 ggcggcggcg cggcagtcgc aggacgccgc gcatgtggca cagggcttcc gcgacgcgtt
     1261 gcgtgacggc gagcgcactg ccggccgccg atggaagcgc atggccgcga tcgctgcggc
     1321 agtgttgatc gggatcgtat cggtgatcgc gtatcgaatg acgtccgtga ccaggccggc
     1381 agcgcctcct tcggcggcgc acacggcgcg catcgactat ggccgcagcg actggaatga
     1441 actggtgaat caggcgctgg caaacggacg cgtcgccata ccggacctga ccgaactgtc
     1501 ggcggccgga ggcactgtcc gatcagagga aggcgccgcg cagaaagtcg atccggaagg
     1561 cgtcgtcgtc gagagcgatc gaccgcgctt ttcgtggccg gccgtgttga aggcagcaac
     1621 gtacgaggtc gtcgtttatc gcggtgagcg ggaagtactc cacagcggca aattgagagt
     1681 cacgacatac gtccctgagc ggtcgctcga gcgcggagcg gtttaccaat ggcaggtgct
     1741 ggtgacggag gagggtgggg cggtgcggat tcttcccacg ggacccgcgg cgcccgcgct
     1801 gatccgcatc ctcccggcgc cggacgctgt cgcgttgacg gatgcacggc ggcacttccc
     1861 tggcgacgcg ctgctcgccg gaacgctcaa ggcgcgatac gggctgctcg acgacgcacg
     1921 ccgcgatctc accgcggcgg cacaacaaca tcccggggat gcgccggtcg cgcgactccg
     1981 cgacacagtc acgaaccatc gttagacacc catgatcctt gccatggttc tcgctgcggt
     2041 ctccttgaac ggccaggccg cgacgcaccg cgatatcgcc ttagccggga tcggtgccct
     2101 gcgcagagcg ctcgccaacg gcgcgcgcga ccgcgagatc cggagcatcc ggcatcgatt
     2161 tccatgcatt gacgtcgcgg atgtgcgcgt gggtccggtc gagatcgatg gcaagtcggc
     2221 cgctgtgtcg ctcgacgtca tggcgcagga agcaggcttt ccttctcatt ggctcgtgcg
     2281 cttgcggcgt ggtcagtccg gctggagagc ggtgcgcttc gacgtggagg agtcgttgct
     2341 ggcggatcgc gtcgcagccg ctgccggcct cgatgcggga cgcagacttc tcgccgagca
     2401 tccggcgctc atcacgcccg agctgtcacg ccggctctcc gagcgcagcg atgaaatcga
     2461 ggaacagagc gacggacgcc acgccgagga ggttgcgcaa ctggcgctgg aagtcgctga
     2521 agattgggat gacgttgccg gtcaggcacg ggcattgtgg gccgcggggc gtgcaaccaa
     2581 tttgctcgac agactcgacg tggcgatccg tgactattcg gaaggctatg cattggccgc
     2641 gcgcgtgaat gacaccgctc tcatggccgc gaatctcgtc ggtctcggga cggagtatct
     2701 gcgcaaaggc gagctcaatc ggacaaagga gctggaggaa gggcttcgtc tggcgcggtt
     2761 ggcgcacgat gatcgcattg cgtggatggc gacgcatcgc ctggccaatg tcgatcgggt
     2821 ggcgggacgc tacgacgcgg ctatccgcaa gtacgaggag gcggtgattc tggcggcgcg
     2881 cgccggagac gccagcggcc aggcagcggc tacctccgac atcggcatca cttacgacta
     2941 catgaacagc cgcgcgctgg cgtatgtcta catccgtcgc ggcgtagcac tctatcggcg
     3001 aaccgccaac acgatcggat tgatccgcaa cattcgcaac atggcggaca tggaagcgtg
     3061 ggacgaacgc tatgcggacg ccgagaagca cttgcgcgag gtagaagctc tgctgcggca
     3121 acatccgaac gaccgtacca gcgcgttcgt ggccgccacg cgctccacgc tagcgatcgc
     3181 ccgccacgac atggccggcg ccgagaaagc gtgcgagcag gcccgcgccc tggccagccg
     3241 gacggacctg ccggatctgg tcacgaacat gaccgaacaa ctggcgggcg tgcgcgtccg
     3301 gcagaaacga tatggcgagg cggcctcact ggcgcgcgag gcgatcgaac gctcacgaac
     3361 aggcacgccg gacttcgaca tctattggca ggcacaactc gaattggggc gggctcttgc
     3421 gggagaaggg aagctcaacg aggcgcgcgc ggcattcgag gattcgattg gcgccattga
     3481 gggaacgctt gccgaggtgc cgcagggaag ggatgaccaa caggcattct tcgccgacaa
     3541 agtgtttccc tattacgaga tgtttcggac gttcatcgct gatcgcccgg aggaggcgat
     3601 cccctgggtc gagcgtgcgc gggctcgtac gttgctcgag tttctggccg cgaagaagcg
     3661 gcgcgcttcg agccgctccc tcaccaccga agagcagacg cgggaggcgg cgatcgagcg
     3721 cggcatcgct gacctgaaca ttcgcatcag cgaagagcgc gcacggtcgc agcgcaatga
     3781 cgcgcagatc gaagcgatcg aacgcgatct cgcggcgaag cgcctggagt tttcggtctt
     3841 cgtccggcgc ctctatgaag cgcatcccga tctggcgatg gcgcggggcg aggtgccggc
     3901 tgcgacggtt gccgagattc gacgcacgat tccggcggac ggcgccgttc tcgaatacat
     3961 ctgtctgccc gaacgatgct gggtcatcgt cctcacgcgc gacgggccgc cgcggatcga
     4021 tgcgctgcaa gcggatcgag gcacgttgca ccgcttgatc ctggagttca gcgccgaatt
     4081 ggcggcacga ggccagggct tccgcgcgaa agggagacgg ctctatgacc ttctcattgc
     4141 gcctgcggac agtgtggtgc gcaacaagag gatcctctgc atcatccctg atgtgccgat
     4201 gggcaatctg ccgtttcaag cattgatcga ccggtacgac aaatacctca tggagcggag
     4261 tgccgtcttc tacgcgccgt cggcggcgtt tcttgtatgg cgtgccacgc atgcgccagc
     4321 ccctccggcg aatgacgccg caaacctcct ggcgttcggc aatccacgga tcgcagccga
     4381 gacgacgcgg gcagcgcatg cgcgaacgcg aagtgagtcg ctcgcttcgc ttcccgaggc
     4441 ggaagaggag gtgcgggcgt tggagcggat gtacggcgcg cacgccacgg ttcgcgtcgg
     4501 tgccgcggca acggaggccg acttcaagaa agaagcgccg catttccgcc tgctgcatct
     4561 cgccacgcac ggtatgtacg acgacgcgga tccgatgtac tcgcatctcg tactggcgcg
     4621 ccgcgccggc gatggcgagg acggcctgct cgaagcgcgc gaagtggcgg atctcgatct
     4681 tcacgccgac ctggtcgttc tctccgcttg tgagaccggg cgtggcggcg agcgggcggg
     4741 cgaggggatg attggtctct cctgggcttt gctcgccgcc ggcagcgaga cggcgatcct
     4801 gaccgacttc aaagtcgatt ccgcggcgag ccgggacctg atgatcgcct ttcatcatcg
     4861 cctgctgcag caaggcggga aacggatgtc cggccgcgtt gcggtcgaag cgttgcgcga
     4921 cgccgagatc gccgcgttga agagccgccg ttcccatccg ttctattggg cttcgttctt
     4981 cgcggccggc ggttggtgat ccgccgttct tgcgcgggcg caccaaaaaa gtgaccgcac
     5041 caatatgccg gcgcagcggc cgtcaaacat ggcgaaggcg agaaggaagt ccgatggttc
     5101 gccgtcttct tagtcgcgtt gtcgtcttcg cggacttgac agagggcgat ctcgatcgcg
     5161 gctttctgat cccgccagga ggaaatatga cgctacgcag gctctctctc gtgttgctcc
     5221 tcgccgtgac cgcgttcgct taccgcgcgc acgcgtgcga gaccgatgcg agtaattact
     5281 cgaccaacca cttcaatccc aatttccgca acaacacgaa ccaggacctc ggcgactacg
     5341 gcctgatctt ccaggatgca atcgatcgca actgctccgg cggaagctgg tcgccgcaac
     5401 aaggcgcgta tcgcgcgctg ctcaacaaga tcaacagcac cttcacctac caggattgcg
     5461 gcagcagtcc cgggatgcac aacacgccct tcgtcggatg ctccgggcca tttcagggct
     5521 ggctcgaggg tgtcaacgtc tctctcatct tctcctccgc gctgcttatc ggtggacacg
     5581 gcgatcttac gacggagctc gaccaggctc tgatgcgcgt ccgcgattcc tacaacggcg
     5641 cgaccagtcc ctactgcggc ttcactgcga gaccggggtt tccgacacag tggtcgcccg
     5701 gcgacacctg tatggacgac tattcgatcg ccgcatcggc gttcgcgtgg attgcgctgt
     5761 acgaacggat gcgcggacgt gacgctacgg cctacgtttc acaagcgcgg ggaaggatcg
     5821 cgcagacgtt ccggacatac gacagcgtct gcatcttcga tcccacggtg gcgatcagct
     5881 ccacacgcgg gcagtgcaat atcgacacgg gcagcatgac ggagtcacag caggcggcct
     5941 ttctcaaaca ggagctcttc ccgaatccca atctcggcgg ccacacactc gcagacatca
     6001 tcgcgctgaa ccgaggcgaa aacatcgttt acggtgcggg actgatgacc gccgtctcct
     6061 ccgcgttcgt cgctctcgac gaaggcggat tctttccctc gctcacggat tcgcaacagg
     6121 tcattgccgc cgcgttcatg gacgaagcgc aacggaagac ggcaacgaac ggcgcgaact
     6181 tcggtttcga ctgcgcgatc ttcgatgccg ctgcaaatcg ctcggataca caaggctgcg
     6241 ccgatggtaa cgccacgcca atgatctacg acctcaattc gaaggcggcc aacctgtact
     6301 accccgacta cggacggagc tcgttctacg acaagtacgt gtcgtacaaa cccgccgatc
     6361 ccgggaccac gatttacgat catgcgaatg gagggtacat cccgatcttc ggtccgttcc
     6421 aattcaacac gttctcggta gcacccttca cgaaggacgc gaacgacacc aacgagaact
     6481 ggggacgtca ggtgttctac cactacctcg gcttcggttg gaacacgatc gacaacacgc
     6541 gctaccaggc aaacaccgac cacacgtccg gcgacgcgcg gccgcggctc tggggatatc
     6601 tcgacgatca cgatccgatc ggatatctcg acggcatcga cgcgaacggc gtcgccatcg
     6661 gctggacgtg tgacaacgat ctgccgacgt ggaccaacgc ggtcgacttc tacgtcaacg
     6721 gaacctcagg tcagcgcatt acgcgcgcat tcgcaaacgt tgcgagcgag ccggctgtca
     6781 acagcctttg cggcggcgga tactcgcatc gcttctccgt acaaatgccg agctggacga
     6841 aaggacagct catctatgcc tacggcctcg acgcgacgtg gcgcggcatc acgctcctca
     6901 acggctggac gtgcgcgcag cacccggcct gtacctggta attgctactc gcgagacacg
     6961 cgcgccctta gctcaatgga tagagcagta gaacttcgga ttctctcaag tcggtaagtg
     7021 tgatgttagt caagcacacc taaaggaggc tgcaaatgaa ggcgagcaag aaagttttcg
     7081 tggcaatggc actggcggtt gttatctgca cttgggggca tcccgtatcg gctgagcccg
     7141 atcagcagag ggtttactac aactgcgtcg atgcctataa cgatccgttg ctcacctgct
     7201 ttggcgacga ttcgtgcatc gacgagtacg gcgatgtcgt gaattgcgca gatatctctg
     7261 gttcacaaac ctgccaacag tatttggtaa cctgcgagag cgcctgtagc agccaggaga
     7321 gtgccgacat gaatgcctgc gcgagaattc aggatccgat cgaccaggcc aactgcatgg
     7381 ccgtcgccta cacgactgcg caggcgtgcg ataacgcgtg tctcggcatt tgcctcaatt
     7441 gattgagagt tgaggggagc agacaagcca tgaacatgcc ccgctgccgc acgtcgctgg
     7501 ctctcgcgat tgtgctcgca cctcttctgg ttcagagtct cgccgcagac gcaggcgcgc
     7561 aggagacgga gaagcgtttc cactaccgtt tcctgcccgt gcctgcgcct ctgacggacg
     7621 acccgcgtat cgtgcgcgcg accgcgatcg tcaagagcag cgatttcaag ctcatcgtcg
     7681 atttcggacg ccggtcctgg ttgatttatg atgcgcaggc cgatcgcgcc cactgcacga
     7741 atcacgagcg catcgagtac gggcccagtg atccttcgca tcccccgctc ctggtggacg
     7801 gtcctatcgc cgattgcgtg gccggaacca tggcgcccgc actgacctcg gtgcgggccg
     7861 gcacgcggac cttcactctc ggaagcgagc agtggaaggc tggcggtccg gccgcactcg
     7921 acgaggagag cggcccggaa atggcgaacg cgatcaagag tctgatcccg gttcttgcgg
     7981 cgctcaaccc atacgtccgg attctctgca accgtatggc gccgctgtac ggcctgaggt
     8041 gtgatgaatc ccagccggca gaggcggcgt acaccgtcag cccgattcct gtggattgcg
     8101 tgtttgacgc acgccacggg gcgccgtgcg tgcgccgatt catgatcgcg ccggcagggc
     8161 taatcaacgg tcattagaag tccgtgaacc acccgcgcgc gtcggcccac cgcgtctcga
     8221 tgcgatagcc ggcgcgcgcg gcgagcgcct cgagcgtcgc gtcggtgatg ccaccgaagt
     8281 ggggatgaca cctgccgagc aacgctggcg ttacttcggg ctcgcgctcg cggctcgcaa
     8341 cgcctggttg gggttgagct ccgccgccgg cgtgagtcgc tccgtcccgt cgccgctgac
     8401 gatgaacggc gcccagtagt acggatgcgc gtactgcggt ctgctacgca gggtcagcgc
     8461 cgcgtgacgc aatgcatcgg cgactgcctc tccgttcctc agacggcgat ggagctcgac
     8521 catcaaaatg gccgtggccc ttgagtcgac gctccactgg ctgacgacgg tggtcgtgca
     8581 gcccgcgatc atgaaagacc acgatagacc gagcattcct tcaccttcat cgacacggcc
     8641 catggcggtg tcgcacgcgg ccaggacggc caggtccgcg ttcaggctca tcgaggcaat
     8701 ctcgcgcgca tcgaggatcc cgtcgtcgtc cgtggtgggg tcgctcatca acacaagggc
     8761 ggagttcatc ggggcgtggt cgtccaggag ggcgtgcgtg ccgacgtgca ggattcggta
     8821 cttcggcgcc tcggtgcgaa aaacgctgga gcgcgcggag gtgcccgtgt acacgcggct
     8881 cgatgcggtt ccgtagagcg ccgcgatgcc gattgcttct ttggcagcat cgggcaatgg
     8941 ccggagatca cctgccgtaa aaccgcgagc gttcgctggc ggtgcaccgc tcgggatggg
     9001 attcccgaac gccatgagat cgtgaccctg gtcgtggttg cgattcctgg cgcgaacgtg
     9061 cgcggcgtga acgtagccga gggatggagc gtagtagatc gagtgggtga ggaggagcgg
     9121 ctctcctgac ccgggggcaa gaacgtggaa ggggagccgc cagagagagc cgttcggaac
     9181 gatacagagg acgcgatacg gcgcgattct ctcgctcacc ggagcgatca acagccggta
     9241 gagatcgtct gcatcgcgtt ggtactcgag atcgcgggag gccagtgacc tgaggaatcg
     9301 gtcgaccttc tcgttgagtg actgctggct gatctcgatc tggtgcgctt cgacgtgcag
     9361 tccgtcatgt tccttgtagg ccacgaatac ggtcgtgcga tcctgttcga cgacgtattc
     9421 caaagcgacc gtgtcggatt cgatgtccat atccatgact ccgagatccg gcaacttgtc
     9481 gactcctcgc cctgaatgct caatggtgcg cgcgacccgg aacctggata gctcgttgcg
     9541 cgcgtcgttg agttttgccg cgagggatga cggttcctcg gcgccgtgaa tcaacattcc
     9601 attcaggtgc tgaatccgct gattcaaggt ggccagggtc ttcctctgct cctcgctcag
     9661 ggagagcatg ggatcactgc cgatgtcctg aatcgatccc agcagcaccc gggctgtggc
     9721 gcgttctgcg tacgacagcg cctcggccag acgtccgccg tccaccagga tacggattgc
     9781 ggacagatgc gccgtccggt gatcgctgaa aaaccgtgcc tgactgtcgg gctcaccgga
     9841 aagactgctt cggcgggttt cgatcaattc gatggcctga tcgagaatgg gcaacgattg
     9901 gtcgggtttc cccacacact ccagcgcatc ggcttctgca accaaggcct ccaattcgct
     9961 tgcattgtgg tcgtggcgcg ccagagacgc cgcctcggcg gcgtcgtctc cggaagcttc
    10021 gaaacgactt tgcgcgtagg cgacacgcga gcgtgcaatg agcgcctcaa tctgtgcggg
    10081 cgtggagtgt gtagcgcggg cccgctccac tgcaatcgac gcccattccg acgcttcctg
    10141 gagtcggttg tggttcatga gcgatccggc cagattggcg ctgttgattg cggcaatcgg
    10201 gcctccgagg tcgcctgcca gctctgctgc gcgctcatat tcacgcaggc tagcgtcaaa
    10261 gtggtggctc atctcgtggc acgcgccgag attcgagtgt gcggacagga gcgttatctt
    10321 gtcgccctcc caatcggcga aatgcacggc agcctgcgcc tcctgaattg cgagatccca
    10381 gtagtcctgc tcgtagtaga tcccgagcat cgtggtatgg gcgtcgaact ccgctcgtcc
    10441 gatcctgcag actgcggcga gctcttgcaa ccgccgcgca agcatcaatg cctgccgttc
    10501 gtcgccgcca gcgtgataga ggtcggccag ggttgccagc gcgttgagag gcacactctg
    10561 gtcgtcaacg aggtgcagca tcgagacaga attctccaaa gacaggatcc ggctccgcac
    10621 gctattcgcc gatcgactcg cacccgcgag tgatgcctcc gcgatgacgt cagtgtcctg
    10681 ggaagctacg gcagctgcac ggcaacgttc aatcaggcgc gattgctgct gcgcatcggc
    10741 taataccgcg cgggcgttga tgcaccacgc gcgtgcgagc tcggcatccg acgttgcgac
    10801 catcagcgcg gcgtcagcaa tgcgtcgacg catcgggatg gcgggatatt gctggagctg
    10861 tttggtaagt gcagtcagaa gcgccgcgga atcgtagccg cctgcgcgta actcaccgtc
    10921 gtagctgtca gagctcgcaa aggtcgccag acgcttggca agcagatctt cgtatgtctc
    10981 gacttccgag attcgccaat gtccctgaac ccgtgcaaac gtcagaatcc agctcttcgg
    11041 gacccctcgg agcctgccgg ttcgcgcgcc ggttgctacc gcatcgatcg taacccggcc
    11101 ctggagtgac ataccgctgc cgatcatgtc gacgcgcggt tgcgaacgga gctcgatgca
    11161 tcgcgacgag gtcaccaggg tttccacgct gttccaggcg gccacataac ggatcggatg
    11221 cgtggcgatg gcgtccagac ttctcatgtc gaggttcacc agagcgcgcg cgaaatcctc
    11281 ggtgagtgcc tgacgagact cggacgcacc gagcacaggc aggcagaatg ccatcagggc
    11341 agcggcgatg acccagcgca tcattcaatg ataaacctgc gtgagtccgt cagtgtgcgg
    11401 ggccggtgct gctccattcc ggcgagtcac atccaactca cttgatcgat ctggtaataa
    11461 tttcgctcgt tttcgtctat cgatggatag ggatcagcgc ctaggatgcg ctgtggtggc
    11521 aaagttggtt gcgcctacca gggagcagtt cgaccagcgt cagatcatcg ccctgaatga
    11581 tcccttgctc cggccgctca tcgatgggcc tgacgagcgg tcgcggcaac gggagatcga
    11641 gcgcctcgtg gtctcgattg cgcggccgat catcgatcgc gttctggttc gccattttgc
    11701 ccggatgatc atgatcagtg gcgaagacgc cgatgacgtt gtggccacgg tgatggtgcg
    11761 gttgatggga aaactgcggc tggtgaccgg atcgatcgac gctgcgatcc gtaacttcga
    11821 tgactacgtt tcggaggtcg ctcatcacgc tgcccgcgac atcgtccgca cccgattccc
    11881 cgccagaacg cggctagaga accgattgcg atatgtcttg acgcgcgact cccggctggc
    11941 gttgtggagt gtgtccggca gttcggtcgc cggcctggcc gcatggaccg atcaggcgtt
    12001 cgtcatgacc gccgatgaac ttgacgccgt caatttcggc ggaatcgttc tcgacccgtc
    12061 gcggccagcg gatgccctgg cagccctctt caagaccatc cggcggccgg ttcgcttttc
    12121 gaatctggtg agcaggatgg cgagtctgtg gcacattcat gatgaaccgg atctcaatac
    12181 gatcgagtcg atcatcgacg agcgccccag tccgctcgct gcgcttcagt cgcggcagct
    12241 catgaacatt ctgtgggacg agatcgtcca gcttccccgc aaccagtgcg ctgcgctgct
    12301 gctgaatctc cgtgactcgg agagcctgga cgcacttgct cttctgccac tcagtggagt
    12361 ggccacgacc agccagatcg ccgatgccat cggcatcggg gtcgatcaat taatgagtct
    12421 ctggaaaagc ctgccccttg aagatcgcgc catcggagaa ctgctcaacg tcacccggca
    12481 acaggtgatc aatcttcgca agtcggcccg ggaacggctc cgccgccgcg ttgcgaaaag
    12541 gacgcgccaa ccatgaaaac acacgcgagc ttcgacgacc tgctggcggt tcggggcagg
    12601 gaattacccg acagagaact ccgcatgatt ctcgatcacg tcgccggctg tccgaagtgt
    12661 tccgcactcg ccacaagagt tctctccctg gacaacgcat cgatcggcct caccgagcaa
    12721 gtcctcggcg tcgacgaaca tctctttgtc gacgaagagc tctatcccta cgtcgaaggg
    12781 atgcttcaga gtgatgcgct ggtaagagcc gagcggcacg tcgcctcctg ctccacctgt
    12841 caggccgaag tcgaggatct tcgtgccgcg cgccgcagcc tgtcccgatc gccggacgcc
    12901 ggccgctggc agttcatggc cgctgccgcc gctgctctcg tgttgctggt gtcgatcgcg
    12961 gtagtgtttt caacgtcccg ccgccacttc ccttccactt ccgtcagacc acgtgcgatc
    13021 actcgtgcaa cgcccgcgaa cgatcgcgat gcgcatggga gtgccgcgtt tgatggtgaa
    13081 gcgctcgacc ctccaccgtt gtatctctct ctcttgtccg gaccggggca gacgcgaggt
    13141 ttcgattcgc cgaggagcag cgcgcgcgct cttcacccgg tcggggagat cgttgaaagt
    13201 tcccgtccgc tgttttcgtg gacatcggtt ccggacgcgg tttacaacgt ctcggtttac
    13261 cgcggcctgc gcccggtcgc gtcgagcgga gagctccatt cgacttcttg gacgacggcc
    13321 gaggatctgc cccgaggagt cacatacacc tggcaggttc gcgtccgcag tcacggcaac
    13381 tccgtggtga ttcctttgcc aacgcagccg atggccaggt ttgcggttct ctcggagaac
    13441 gcggaatccc aactcgagga ggctcggcgc aatcgccccg acgatcatcg atcgctcggc
    13501 gtcctctatg cccgcctggg aatgctcgag gatgcccgga gcgagctccg aaaggcagca
    13561 gccgatccag cgcaacgcga tcgggtcact ccgcttctcg aaagcctcga tcgctggcca
    13621 acgtcggagt aataacggct cgcaggccgc ggtaatcatc accgcctttt accgtcccgg
    13681 aagacttcct cagctccctc gtatagtgca gaaaaccttg ggaaaggacg atgggaagat
    13741 gaagagaatg aacgcaatcg tgttgttgat cgccacactt actctcgctt tacccctctc
    13801 tgcggcgcct gccggcggga gcggtgacac gttcctggca cgcctgctac gcacaatcgg
    13861 acgcgttaca cacgctctcg acgagccgca agggccgcca ccaagtaacg ggcgaccctg
    13921 agtctccgag agatccaatt cgagttacga gccctcactt tcacactcac aagttgaacg
    13981 gcgattcgcg cggctggttc gtgccgccag aacgccgaaa aggagaccga gccgatgaag
    14041 aaaatgaatg cagtcctgct gctgatcgcc actctcgcgc tgtcaatgcc agcagccgca
    14101 tcacctcgcg accggagcgg cgactcaatg ctggggcgta tgctccagag gttgacccaa
    14161 gtcattcaca cgctggaagg cccgatgatc ccccctcccg gacacgggta gccccatcct
    14221 ccgccgcggc cctcgttaga aggtgggccg cggcggtgag ggggctcagt tgacgacgat
    14281 gctgaccagc tttcgcggca cgatgatgac acgttccaga ttcgcacctt ggacgtattt
    14341 cgcgacgttc ggctcggcga acgcaagctg ccgcaattga tcgtcggttg aatccgcgct
    14401 cacagtcagc ttcgcgcgca gtttgccatt gacctgtacc gcgacagtga cctcgtcctc
    14461 tgccacgagc ccttcgctcc acgccggcca ggattgtgcg tgcaccgatt ctgtgccgcc
    14521 gatgcgctcc cataactcgt ctgcaatgtg cggcgcgacg ggcgccagca aacgcaggta
    14581 tgtctcccgg gcctcacgcc acgcttcggg cgaacagccc tcgttgagcg caagggtgag
    14641 ctcgcgatgc aacgtcatga gggcggcgat gatcgtattg aactcgaacg actcgaaatc
    14701 gtgcgtgacc gagcggatgg tctgatggag cttgcggcgg agccgccgcg tggcctcgtc
    14761 gttcgttcct gccggatctt gttcgtcgac gaacgacatc cagacccggc gcagccagcg
    14821 tgccgttccg gagattccct cgctgctcca tggtccgccc tggtcccaac gcgcgaagaa
    14881 catcaggtgc gcccgcatcg tatcggcgcc gtagcggacc agcagttcgt ccggcgcgac
    14941 gacgttgcct tggctcttcg acatcttttc cgagtcttcg ccgaggatga tgccctggtt
    15001 gcgcagttga agcatgggct ccggctcgcg ggtgacgccg gcgtcgcgca gcgccttgtg
    15061 gaagaatcgc gtgtagatga ggtgcatcgt ggcgtgctcg atgccgccgg tgtaggtatc
    15121 gaccggcatc cagtagtcgt attcgtcgcg gtcgaatggc cagtcgtcgc agtcagggct
    15181 caggtagcgg aggtggtacc acgacgaaca gaggaatgtg tcaagcgtgt cggtctcgcg
    15241 ctcggccgga ccaccacaac gcggacaggt cgtgtgccgc caagtcgggt gtagcttcag
    15301 gggactctcg ccggtcggac gccattcgac gtcgtcgggc agcgtgacgg gcaactgatc
    15361 ctccggtact ggaacgatgc cgcaagccgg gcagtagatg atcggaatcg gcgatcccca
    15421 gtagcgctgc cgcgaaacga gccagtcgcg cagccggtac tgcacgcctc gacggcccgc
    15481 gtccaatgct tcgagatgtt cgatcacttc gttgatggcg cgatctgcgg agacgccgct
    15541 cagcgggccg gagttgatca tgcggccatg ctccgtatac gcggcatcga gcggctcggg
    15601 aaggcgatcg ccgtcttcgg gctctatgac ggtacggatc tcgaggccga acttccgggc
    15661 gaacgcgaag tcgcgctcgt cgtgcgcggg gacgcccatc accgctccgg ttccgtatga
    15721 ggcaaggaca tagtcggcta cccagatcgg gatccgctcg cttgtcaccg ggctgatggc
    15781 gtagtggccg gtgaatacgc cggtcttctc acggtcggcg gcctcccgct ggatgtccga
    15841 ctgacgcgcg gtctggtcga gatattgtgc gacttcgggt gattcctctc cccacttcct
    15901 ggccagctca tgttccggag cgatggtcag gaaggtgact ccccaaagtg tgtcgggccg
    15961 ggtggtgaac acttccaatt tcgcgccggc atcgatggcg aaggcgatcc agcttccggt
    16021 ggagcggccg atccagttct tctgcagggt tcgcactctt tccggccagt cgatctggga
    16081 aaagtcgagc agctcttcga cataccgcgt cacgcggaag aaccattgcg cgagctcctt
    16141 gcgtgtgacc ggcgtcttgc agcgctcgca gtgacgatca tcgccccaca cctgttcgcg
    16201 cgccagtgtc gtattgcagc tcgggcacca gtccaccgcc gcgctcttgc ggtaagcgag
    16261 gtcgccatgg aacagcttca ggaatagcca ctgcgtccag cggtaatact ttggatcagc
    16321 agaaaccatt tcccggcgcc agtcgaacat cgctcccatt gagcggagct gtctgcgcat
    16381 ccgctcgatg ttgcggtagg tccactcttt cggatggatg ttgttcttga tggccgcgtt
    16441 ctccgcgggc agcccgaagg catcgaaccc catcggaaaa aggacgttgt agccgcgcat
    16501 gcgcatgaag cgcgcgcggg catcgggcgg tgccatcgag taccagtgcc ccatgtgcag
    16561 atcgcccgag gggtagggga gcatcgtcaa cgcgtaatgc ttcgggcgcg tacgatcgat
    16621 atcggatcga tcgattccgc tctcggccca gcgctgttgc cacttggctt cgatctcatc
    16681 gtgcttgtaa tcgttgggag tcggttcgtt catcggttca ccgccttaca gaacgcagga
    16741 tcggcggcgc gcgttccgtt gcgtctcctc tgtccgaagg atcatgtcag gctccactgt
    16801 gtcacgtcgg ccttctccag gatctcgccc acgggaaaac tcgtctcgag tgtcgattga
    16861 acccgggcat tgatgagctg ctcaaccctt ttccacacgg tttccccgtt cgctccgctg
    16921 ccattcgtca cgcccaggac gacgtcgtcc acgagctttt cgttccggga gatgatcgtc
    16981 ttcaggatct gctctttcac ccccggggcg ttggattgtt cgccttttgc ccgggtgagc
    17041 acgtcaatga ctgccagcgt ccagccgccg ccgcttgcgg cttcatccgg ccaggcccaa
    17101 ccacctgcgt ggaaaatctt ttgcagtgat tcgatcttca ggttctcctt gctgctctcc
    17161 tcgctgtccc tgagcttcag gaaattgttg gccgcaccgc aaaagagccg cactccgcag
    17221 tcgagaagct tttgcacgtg ctcgggacgg gtgttatcgc cttcgccgtt attgatccag
    17281 acgtcgtagc tctggctgtt gtcaagaccc ttgtagaaat cttcgtgggt catcgccctg
    17341 gccttgatcc cgtatttgtc ggatacctgt ttgcagacct cggcagactt gtcgaaggca
    17401 gtaatctgat tgggagagat ccctttctct ttgatcagcg cctcgaccgt ggcctgacca
    17461 acaccgccca gtgcttccag gagaaactgt atccctgctc tatcccggtc ctcgaatcga
    17521 tagctgacca gcttgagcgc ggtggccacg acgccatatc cggtcggata ggcggacgtc
    17581 acgcgtgggc cgccccgagg ttcgggcagg caggttacga gcgaaagatc gataccgcgc
    17641 ttcgcccata catcgcacat ggccttccag ttcggtccgg acagcacgtc aggcgacgct
    17701 gtatccggac gcatgaagtt tcgcgatggg aaggagcggt aaatgccttt ctcaatagcg
    17761 actttttctt tgtatccatt caagaacgcg attcccttgg gaatgatcag cgtgcccaat
    17821 gccacaatcg cttcctcgga aataccgtcg attttcctcc cggcccggtc cacaatgacc
    17881 cattcaccct tcttcccgcc gtgtaaagtt agaatgtccg ggaagaccag cacggctttg
    17941 cggatcatgg cctgggcgag gttctttgtc cggctcagaa cggtctcgag gtcccgaatc
    18001 tcgctgccgg tgcggtagca gtcgccgccg atgaagatgg tcagttcctc gaacggcttg
    18061 tgattgaagt gggtgacggc gtagacgacg tacacctttt cttcgtgctg caggagcgca
    18121 tccagttcag agatgattcg aaaccggtgc tcgaattctc tggccgcctt gtcgatcatg
    18181 agcggtgtca actgcatgat cgtggcagta atcgagtaga ccttctcacg ctgacggagc
    18241 tctgagagcg attccaatcc gtctatttcc tgaagcgacg atggatcgaa ggtggtggtt
    18301 ccgaagactt tcccttccag ttgttcgttg caaatcagga cgatttgatc ggcgttgaaa
    18361 cggctgagaa tctccgccgg aatcaggttg atcgagagag atgccatctt gcttcatcct
    18421 ccgtcggggt tactttggag tgcacagcct tcatcgctgg catccgctag tacgtgagag
    18481 cgccatcgcc ggccggcgtt gatgccggtt tccctgggtc gagcgcgacg cacgagcagc
    18541 ggactttcga ggtggcacgc acgaagaata tcagagtgca cgggaagagc cccgagcgtt
    18601 tttcttgctg ttgcagaggt cgggtaagat cacgacgcga tcttagcgtc ccggcctcgg
    18661 tgagaaaacc aacgccgcat cgctatttct gagggagggg atgaatcgtg tctttcgaaa
    18721 aacagctcgc tgatcaggga tacacgcttg caccactaaa cctcgacgcg ggacgactgc
    18781 ttcacgcgac ccgcagcggc aacctggtct acacctcagg ccaggttccg gtgctcgatg
    18841 atcgttcgat caagggaaag gtgggtagcg acctgacgat cgaagaggcg caggaagccg
    18901 cgcggctctg cacgatcaac tgcctccggg ccgtgcagtc gctcgccggc tcaatcgacc
    18961 tcatcgcccg gattgtgaag gtcttcggca tggtgaacgt cgcccccggc tttgatcaga
    19021 cgccatccgt gatcgacggc tgcagcgacc ttctcatcag cgtcttcggc gaggccggca
    19081 aacacgcacg aagcgccgta ggcatgacca tccctcttaa cttcgccgtg gaaatcgaaa
    19141 tgatcgtcga gttacgggcc taagttctcc aggcgccctc cgcgttgatc cagttttctt
    19201 gccgaggtgg ccgggggccg gctacggctc agggtgaacg tgcccctttt tgcaaacacc
    19261 aacgtgttta catacatcgc ccgcaagcct cacatcaagc ttgtctatta gtctctctac
    19321 cgacttcaga gcaccctcaa tccagccctg ctcactcgag tacgcctcgc cacaaacgaa
    19381 cactctgtgc ttcccttgat tgccgtgtcc gtcggggaaa ggctgcgcca tctcctccat
    19441 agtcagttcc gagttcgcgc ctggctccca cgtgtgccag cctgcctctg gtataggctc
    19501 atctccgaaa ttgtcccaga acatataggc acccacgacg ggcggttcgg gcgtgacctt
    19561 gtgtccgttc ttgagagacc cgtgcaggtc tctgagtatg tgcgtcatct tgtcggacgc
    19621 gcgtgcaaac aggccgtgtt tccgaaaata caaatatcga acccgctcct cgtcagtcca
    19681 gggctttaac ggctcttctt ccttcaaccg tccataaaac tccctcattc tcgcgacgtt
    19741 accctccttg ctatcgtcct cccatctcct cggagatacc tcgggccgat ccgaccagcc
    19801 ccaaattcga tcgccgaagc atttgattct cttctcttcg gcggtctcag cgcatttgat
    19861 tctcttgatt atcttctctt cgtcagtctc cctcggttcg atcgatccgt cttcgtcctc
    19921 acgatacgcg tccaggcggg cattgagtcg ctggaccgtc gtgatgaacc gccagtaaga
    19981 gacgtaatga ccgtcaaggt acgccacgat aatgccaccg atgtccggtt gtcttacagg
    20041 agcgtcagtc gtgacgttgc ccttcacata gaactcgtac cgacccctct tttcgagcca
    20101 ctcttgatcc cagtaataga tttggcgtaa aggcaagtcg gtgatgatta gttcgcccgc
    20161 tccctttgcg cgttcgggag accgccacca gggagtgcga aatgcctgca ccagtttggc
    20221 caatcgatgt gcccgcactg tatcgagatc ccccagccac ttcctcttgt gtacccgatt
    20281 taacttgtgt actcgattta actctttcgt tggtttgcca tcatcggaag gccagtcgct
    20341 taacagatca tcgacgtgca gtttagacag cggttctttg ggaatggcaa gtatgacggc
    20401 ccgcgcgctg aattgccacc atgtcagttc tttggctttc tttaccgcgt ctgactcttc
    20461 ggatgcggcc acacggtcat cgtcattttg atgttcgtaa cgcaacgctg tcgtggtggc
    20521 gagcacgctg tagatgtctg cccccaagtc aaacccgatc tcccgcacac gtgtgttgag
    20581 gtatatctca caggtaaatg tggggtgttt cttcagttct tccgagtccg tcaaagccgc
    20641 cttaagtcgc acgatcaggt cgccgaagcc tttttccacc gcttcatacg attgccctaa
    20701 gctgaagtcg tcgatgaacc acggaatggc gtccgagaga ttccaattgc cgaggaccga
    20761 ttcgtagcca aacccatcct cgaccagttt ggctccgtct ggactcagat agtggtgtag
    20821 aaggttccag agtccaatat ctctgagatg tattttgccg cgcagcagac agtggtcctg
    20881 gatctccaac cattctctat gggttagcgt ctctcgcaag gctttgtctc ggagtttctc
    20941 cagcgctcgc agcgtagaaa gatagtcggt gtccagctgc ctttttgctc ttgcgaccgc
    21001 tttacggtcg ctctccgttt tcagaatatc cgtcagtgcc cgacgagttg ggtgtgctat
    21061 ataatgcttt ttcgcaaaga ccgaaccatc gtcgaaatag gaatcagaat cgattgacag
    21121 ttctgccaca gtgctgtcga acaccatctg cactaactct tccaagtgtt tgttctgttc
    21181 cggacggtca gatgtgtttt gtaggctgta tggcgacttg ccttgcttga cgtcggcatc
    21241 ccgaaaatgt tttcctcgga ggaagtaact cgtatcaaaa ccatcgcctt tgaatggcat
    21301 tgttccgatt ttgagatcgt taaggagccg gcgcacgagc ttctgacggg ttgtaaacct
    21361 catcggtccc acctcagcgg tcaccgctcc gccgggaaac ggcaatttga ccgcgcgaac
    21421 acggcctccg agctcaagag ggttctcttc gagaatggcc acgcgaagtt tcttaccctt
    21481 cacattaagg cggtctttaa ccgttttgcg cgtcttggcg tcgcctgact cccacacttg
    21541 acgcaaacga tacgctgcat acagacctgc tataccgccg ccgacgatca cgacgtcgta
    21601 tagcccccta gtttgctcgt ccgcagaggt cgttgtgcta gaagtcatcg catcctcttt
    21661 ctctgaccgc gtggtttagt tgccaaaagt tggcgggact acttactgtc atgggctagg
    21721 cgccgtcgcc ctcggtcgaa gtcttgtcag ggaaccacca cagcgtcttt aaatacgatt
    21781 tttggcgatc gacctcaggg aagagcgtgc cctcgtggat gccgagcttg aagagctcct
    21841 caaggatcgg tttcttggcg cacttgggga tcgagaagcg cttgataagt ggagcttgct
    21901 catcgtcatc cagctcggcg atgctgatcg gttccgggat tgtgtttccg agataatgtc
    21961 ttctgagact tgggacgtac ttcttgcccc cgtgcagagt gaagacactc gcttgataag
    22021 tcattcgatc attaagccga ctcgggaaaa c
//
LOCUS       DATLVW010000908         7583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_408709, whole genome shotgun sequence.
ACCESSION   DATLVW010000908 DATLVW010000000
VERSION     DATLVW010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408709"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5333
                     /locus_tag="VNN25_26990"
     CDS             <1..5333
                     /locus_tag="VNN25_26990"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXH95250.1"
                     /translation="QITGISGTTITLSTAVSGAIGANVITTEHYTISYANTGAAGTGL
                     TLTDFLPANLLYAGVPTGALNPTSSPVVGANGNIVWDLSNIVSNVAVTAGGSGYGSAP
                     TVIFTGGGGAGAAATATISGGIVTSITITNAGHGYTSAPAVSFSSGSATATASIATSL
                     TNGATGSREFLAYPNAAGTYTNAATISDGTAANTRNASDTAITTFGALSPAKITNTPQ
                     VTNSGSDVATYVITVQNPLPSTTATSVKITDNLPSGFTYINGTTKINNVAAADPSCSG
                     STSSSPIWCGVAFNIAPNSTLTLQFNALVAANVATGSYDNEVLVSSSIPSLIFDYLAT
                     PAENVQVCDTAPPITAPAVCASSTGNVASIGILPSTTYSWSITNGTITNFNTGTVDRV
                     AIGSGGTGYTTPTISFTGGGGGVGATATATVSGGVITAITIVNPGSGYTSAPTVVITG
                     SSTTAATTQAVLGRGIIYTAASSGPVGLQVILTSGTCTATQTKSVTVNSAPSITTDTS
                     SKTACSGASVTFSAVFSGANSFQWQDSSDGITWTNIAGATTSSHTVTAGTAPDVDGHQ
                     YRVIAASGALLSGCLTTSSAATLHISCTLDLEVTSDIASPSPVSAGRNLTLTQIVSNV
                     SGNATTATVTFSQSVPANTTFVSMTPPAGWSCTTPAVGGTGAITCTTNSGVHLNANTS
                     TGSFTLVLATDPTTVDGTVATDTATVAVGAGDTDSVATNNSKSTSVTIQRQIDVQVVV
                     NDTANAYGPHFINPGNPPTPQSMDWIINVGNGGPSMGSNVVITDTIPSGFTYTGFSES
                     NLNGTCAPSSGNTTLTCTVGNLMPSTGLTLVGGTGSGASVYPTVSGGQITGITIVNGG
                     SGYGTTPPTVVFSGAGTGAVATAILTNGVITGFTISNPGSGYVTPGTITIHGTVNAVQ
                     TQIVDSAAATYNETDTNPSNDSSADTVSILAPTVVKILTMDGKQDRNGVTLTWKTTFE
                     ADNLGFYIWRESANGTREKISPNIIPGSALFTGKGIAAKTNRSYRFVDRAAKDLQFFQ
                     YYIEDVDLKGVHTMHGPVSPVAAPTGTTSAVTTDPDPTLGSVGGIFTTAPGMGVVAPA
                     PTAPDSQRLSQQWTLAGSPAAKLIVTQPGWYQIKKSDLVAAGFDPGTNSRNLALFADG
                     IEVPILVPNGSFGATDTIEFYGTGIDTPNTGGHVYYLTTKTGSGLRVKSASKVSGGSS
                     APANFTYGFDRTERTGFFAALTNNGDRENFFGAFVTTYPVTNSLTVSNLDPQGGNATV
                     RIVLQGANSDPFSHIVDANINGTDLGLIRYNGQARMVADLNVPLSALVNGENTLTLTA
                     MGGDDDFSALETVHLSYPHTYRADTDALEFTAPASSAITVTGFTKNTIRVVDLTDPQN
                     PTLVGTTITQAADGTSSAAFTTGGTGTRTLLAFADDRVLPPAQVALNEVSTLNAATNA
                     ADLVIITNHAFATAAASLQSARAAQGIKTVVVDVQNVYDEFSYGVHGPEAIRSFLQRA
                     NTSWTSHPKWVVLLGDASFDPRNYLGMGAFDFVPTKLVPTQYLKTASDDWFTDFSGTA
                     TAVIPVGRIPVRTADEANAVISKLVRRTTTPPSGPWASTVTLISDRVNGVPFEQGSDQ
                     LAQLVGPPYQTNRLSFASLSDPSTAVVNAFTNGNLLTNYLGHGSVEIWSDYVFTSTAA
                     AALANGDKLPFVVTMNCLNGYFHDVYTESLAEALLKNANGGAIGVWSSSALTSPDEQL
                     KVNLELYRQIFGSTSITIGDAILKAKLVTTDSDVRRTWILFGDPSMKLRP"
     gene            5407..>7583
                     /locus_tag="VNN25_26995"
     CDS             5407..>7583
                     /locus_tag="VNN25_26995"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXH95251.1"
                     /translation="MKQASLRFIRLVIVPVALALVCTASAFAQSQLRDFSVTQDKTNV
                     LISWQMNCEDGNAGFNVYREVSGQRTKINQHLIAGTMLGAAKHDSKAGNVYRFSDKLD
                     EGTFAQYWLEDIDTAGRHTMFGPVSPVSGRVSVMPNTMLTGLGSGGSEFTTPDGFGAA
                     HATAQEPASATQYAQQRDLASENGLKIFVSQEGWYRLTRSAMTAAGFDPGPDAHALSL
                     YMLGNEQSIIVDDGGDGKFDPNDAIEFYGYPLDSVSTGARTYWLRPKKGSEPRVTTSK
                     IKGGDPITGNVSFLYERIERSGFFAALTTNGEADNFFGEVIGDWTATEPLRVGNFDPA
                     YGGNATLDLVIQGGSLMEHRIAVAINGHDLSTVVLQNTEQPTFTFQVPQSWLVSGLNA
                     VTLTSLNGDDDISGMAKSRLTYQHLLRADNGALDVSLPGGRSVTIGGFGAKTVRAFDV
                     TDPRHPTELQATVAADPQGGFAATFSTLAGGMRTVLAIDGTRLLGHGELAANAPSSWS
                     ASSNNPKSPAFFIISHGSFLGAAATLKALRDSQGISTAVVDVDDLYDEFNFGIRSPEA
                     IRSFFRAAAGWKHPPSAALLLGDASMDPRNYLGLASVDFVPTKLVPTLYMKTASDDWF
                     TDFNGDGIADIPVGRIPVRTPQEASLVIGKITSRGTPSGAWSKSALFVADSPVDFDFP
                     GVAASLTTLLPSSITSQTIDFSRTSSPHADVVNGMNNGSLLVT"
BASE COUNT         1504 a         2551 c         2157 g         1371 t
ORIGIN      
        1 cgcagatcac tggtatcagc ggcacgacca tcactctcag cacggccgtc agcggtgcga
       61 tcggagcgaa tgtcatcacc accgagcact acacgatcag ctacgccaac accggggcgg
      121 ccggcaccgg cctgaccctg acagacttcc tccccgcgaa ccttctttat gcaggcgttc
      181 cgaccggcgc tctcaatccg acctcctcac cggtagttgg tgccaacgga aacatcgtct
      241 gggatctgag taacatcgtt tcgaacgtag ccgtcaccgc gggtggatcg gggtatggat
      301 cggcacccac tgtcattttc acaggtggcg gtggcgccgg cgcggcggca acggcaacga
      361 tctcgggtgg catcgtcacc tccatcacga ttaccaacgc aggccacggg tatacctccg
      421 ctccggccgt ttccttctca tccggcagcg cgacagcaac tgcgtcgatt gcgacgtctc
      481 tcacgaacgg agcaaccggc tcccgtgaat tccttgccta tcccaacgct gccggcacct
      541 acaccaatgc ggcgaccatc agcgacggca cggcagcgaa caccagaaat gcgtccgaca
      601 cggcgatcac cacgttcggt gccctcagcc cggcgaagat cacgaataca cctcaggtga
      661 ccaacagcgg gtccgacgtc gcgacgtacg tcatcaccgt gcagaacccg ctgccttcga
      721 ccacagcgac cagcgtcaag atcaccgaca acctgccgag cggattcacc tacatcaacg
      781 gaacgacaaa gatcaataac gtcgctgccg ccgatccttc ctgctcgggt agcacgtcgt
      841 cttcacccat ctggtgcggc gttgcgttca atatcgcgcc caacagcacc ctgactctcc
      901 agttcaacgc actcgttgcc gccaacgtgg caacaggcag ctacgacaac gaggttctgg
      961 tgagctcatc gattccttcg ctcatcttcg actatctggc caccccggca gagaacgtcc
     1021 aggtctgcga cacggccccg cccatcacgg cccctgcggt ctgcgccagt tcgacaggca
     1081 acgttgcctc aatcggcatc ctgccgtcga ccacgtatag ctggtccatt accaacggca
     1141 cgatcacgaa cttcaatacc ggcacagtcg accgcgtcgc gatcggcagc ggcggcaccg
     1201 gctacacgac accgacgatc agcttcacag gcggcggtgg cggagtcggc gccacggcga
     1261 ccgccacggt ttccggcggc gtgatcaccg cgatcacgat cgtcaatcca ggcagcggct
     1321 acacatccgc accgaccgtg gtcatcacag gcagcagcac gacagccgcc accacgcagg
     1381 ccgtcctcgg ccgcggcatc atctataccg ccgccagctc cggaccggtc gggcttcagg
     1441 tcatcctgac ttcgggaacg tgcacggcca cgcagacgaa gagcgttacc gtcaactccg
     1501 caccatcgat taccaccgat acgtcatcga agactgcctg ctccggagcg tccgtgacat
     1561 tcagcgccgt gttcagcggt gccaattcat tccagtggca ggattcgagc gatggcatca
     1621 cctggaccaa cattgcaggt gcgacgacga gctcccacac tgtcactgcg ggtaccgccc
     1681 cggatgttga cgggcatcaa tatcgcgtca tcgctgccag cggcgccctg ctgagcggat
     1741 gtctcaccac cagcagcgcc gcgacactac acatcagttg caccctcgat ctggaagtga
     1801 cgagcgacat cgcatcgcca tcgcccgtgt ccgcgggacg gaatctgacg ctgacgcaga
     1861 tcgtctcgaa cgtcagcggc aacgccacga ccgccaccgt gacgttctcg cagtcagtcc
     1921 ccgccaacac gacattcgtg tcgatgacac cgcctgccgg ttggagctgc accactcccg
     1981 cagtcggagg caccggtgcg atcacctgca ccacgaactc gggcgttcat ctcaacgcga
     2041 acacctctac gggaagcttc acgctggttc tcgcaacgga tccgaccacc gtggacggaa
     2101 ccgtcgccac cgataccgcg acggttgccg tcggagccgg cgataccgac tccgtcgcca
     2161 cgaacaattc gaagtccacg tccgtaacga tccagcgcca gatcgacgtg caggttgtcg
     2221 tcaacgacac ggccaacgcc tacggccctc acttcatcaa tccgggcaat ccacccacgc
     2281 cgcaatcgat ggactggatc atcaacgtcg gtaatggcgg cccatcgatg ggatcgaatg
     2341 tcgtcatcac cgacacgatt ccgagcggct tcacatatac cggcttcagc gaaagcaacc
     2401 tcaatggaac gtgcgcgccc agcagcggca acacgacgct gacctgcacg gtcggtaacc
     2461 tgatgccgag caccggcctg acgctcgtcg gcggcaccgg aagcggcgcc agcgtgtatc
     2521 caaccgtttc gggtggccag atcacaggaa tcacgatcgt caacggcggc agcgggtatg
     2581 gcacgacgcc cccgacggtc gtcttctcgg gcgctggaac gggcgccgtg gctaccgcca
     2641 tcctcaccaa cggtgtgatc accggattca ccatctcgaa ccctggctcc ggctacgtta
     2701 cgccgggcac gatcaccatc cacggcacag tgaacgccgt gcagacgcag atcgtcgaca
     2761 gcgctgccgc gacgtacaac gagaccgata ccaatccttc gaatgattcg agcgcagata
     2821 ccgtgagcat ccttgcaccc accgtggtca agattctgac catggacggc aagcaggatc
     2881 gcaacggcgt cacgctgacg tggaagacga cgttcgaggc cgacaacctc ggcttctata
     2941 tctggcgtga gtcggcgaat gggacccgcg agaagatcag cccgaacatc attcccggct
     3001 cggcgctctt caccggcaag gggatcgcgg cgaaaacgaa tcgcagctat cgcttcgtcg
     3061 accgcgccgc caaggatctg cagttcttcc agtactacat cgaggacgtc gatctcaaag
     3121 gcgtccatac gatgcacggg ccggtctcac cggtcgccgc tccgaccggt actacatctg
     3181 cggttaccac cgatcccgat ccgacgctcg gctctgtcgg cggcatcttt acgaccgcac
     3241 ccggcatggg cgtcgtcgcg ccggcgccta ccgcgcccga ctcgcagcgc ctcagtcagc
     3301 agtggactct cgccggttcg ccggcggcga agctgatcgt gacccagccc ggctggtatc
     3361 agatcaagaa gagcgacctt gtggctgcag gattcgatcc gggaacgaac agccggaacc
     3421 ttgcgctctt cgcggatggc atcgaggtgc cgatcctcgt gccgaacggc tcgtttggcg
     3481 cgacggacac gatcgagttc tacggcacgg gcatcgatac accaaacacc ggcggccatg
     3541 tctactacct gacgacgaag accggcagcg ggcttcgcgt caagagcgcc tcaaaggtga
     3601 gcggcggcag cagcgcaccg gcgaacttca cgtacggctt cgaccgcacc gagagaaccg
     3661 gcttctttgc ggcactgacg aacaacggcg acagggagaa cttctttggt gcgttcgtca
     3721 ccacgtatcc agtcacgaat tcgctgacgg tctcgaatct cgatcctcaa ggtggtaacg
     3781 ccaccgtgcg gatcgtgctg cagggcgcca acagcgatcc gttcagccac atcgtcgacg
     3841 caaacattaa cggtaccgac ctcggcctga tccgctacaa cggtcaggcg cggatggtcg
     3901 cagacctgaa cgttccgctg tctgctctgg taaacggtga gaacacgctc accctcacgg
     3961 cgatgggtgg cgacgacgac ttcagcgccc tcgagacggt tcacctctcg tatccacaca
     4021 cctatcgcgc cgacaccgat gcactggaat tcaccgctcc cgcatcgtcg gccatcactg
     4081 tgaccggctt cacgaagaac acgattcgcg tagtcgacct gacagaccct cagaatccga
     4141 cgctggttgg taccaccatc acccaggccg ccgacggcac cagctccgcg gcattcacga
     4201 ccggaggaac gggcaccagg acgctgcttg cgtttgccga tgaccgcgtc ctgccgccgg
     4261 ctcaggtcgc gctcaacgaa gtttccacgc tgaacgcagc cacgaacgct gcggatctgg
     4321 tcatcatcac gaaccacgca ttcgccacgg ccgcagcttc actgcaatcg gcgcgtgccg
     4381 cgcagggcat caagacagtc gttgtcgatg tgcagaatgt ctacgacgaa ttctcttacg
     4441 gcgtgcatgg ccccgaggcc atccgctcgt tcctgcagcg cgccaacaca tcgtggacga
     4501 gccatccgaa gtgggttgtg ctcctcggcg acgccagttt cgatcctcgc aattatctcg
     4561 gaatgggtgc cttcgatttc gttccgacca agttggtgcc gacgcagtac ctcaagacgg
     4621 cttccgacga ttggttcacg gacttcagcg gtaccgccac tgccgtgatc ccggtcgggc
     4681 gtattccggt gcgaacggct gacgaggcca acgctgtgat cagcaagctc gttcgccgca
     4741 ccaccacacc tccgtccggc ccgtgggcgt cgacggtcac gttgatctcc gaccgcgtca
     4801 acggcgtacc gttcgagcag ggcagcgatc aactcgccca gctcgttggg cccccgtacc
     4861 agacgaaccg cctgtcgttt gcatcgctca gcgacccgtc cacagcggtt gtgaacgcct
     4921 ttacgaatgg caacctgctc acaaactatc tgggtcatgg ctccgtcgag atctggagcg
     4981 attacgtctt cacgtcaacc gccgccgccg cgctcgcgaa cggcgacaag ctgccgttcg
     5041 tcgtgacgat gaactgcctc aacggctact tccacgatgt ctacaccgag agccttgccg
     5101 aggcgctgct caagaatgcg aacggcggcg cgatcggcgt ctggtcgtca tcggcgctga
     5161 cttcgccgga tgaacagttg aaggtgaacc tcgagctgta ccggcagatc ttcggcagta
     5221 cctcgatcac gattggcgac gccatcctga aagcgaagct cgtcacgacc gacagcgacg
     5281 tccgccgtac gtggatcctc ttcggcgatc cgtcgatgaa acttcggccc tagcagctct
     5341 tcactcaagg ggaggcggac caaacggccc gcctcccttc gtgccgttca actaaggagc
     5401 ctgggtgtga agcaagcatc tcttcgtttc atccgtttgg tcatcgttcc cgtcgccctt
     5461 gcgcttgtgt gcacggcttc ggcgttcgct cagtcccaac tgcgcgactt cagcgtcacg
     5521 caagacaaaa cgaacgttct gatctcatgg cagatgaact gcgaggacgg caatgccggc
     5581 ttcaatgtct atcgtgaggt ctccggacag cggacgaaga tcaatcagca tctcatcgcc
     5641 ggcacgatgc tcggagcggc gaaacacgac tccaaggccg ggaatgttta ccggttcagc
     5701 gacaaactgg acgaggggac gtttgcacag tattggctcg aggacatcga tactgccgga
     5761 cggcacacga tgttcggccc cgtctctccc gtctcgggtc gggtttccgt catgccgaat
     5821 accatgctta cagggctcgg ctccgggggt agtgagttca ccacgccgga cggtttcggc
     5881 gcggcgcacg ccaccgcgca ggagcctgct tctgcgacgc aatacgcgca acaacgtgat
     5941 ctggcttccg agaacggcct caagattttt gtgagtcagg aaggctggta ccgcctgacg
     6001 cgctccgcca tgacggccgc tggttttgat ccgggtcctg atgcccacgc gctttcgctc
     6061 tacatgctcg gtaacgagca gtcgatcatc gtcgacgatg gcggcgacgg taagttcgat
     6121 ccgaacgacg ccatcgagtt ttacggctac ccgctcgatt cggtctccac cggcgcgcgc
     6181 acgtactggc tccgcccgaa gaaggggagc gagccgcggg tgaccacatc aaagatcaag
     6241 ggtggtgatc cgatcaccgg caacgtttcg ttcctctacg agaggatcga acgaagcggc
     6301 ttctttgcgg cgctgacgac gaacggtgag gctgataact ttttcggaga ggtgatcggc
     6361 gactggacgg ctaccgagcc gctgagggtc ggaaatttcg acccggcgta tggcggcaac
     6421 gccacgctcg acctcgtcat tcagggcggt tctctcatgg agcaccgtat cgccgtcgcg
     6481 atcaacgggc atgacctgag caccgttgtc ctgcagaaca ccgagcagcc tacattcacg
     6541 ttccaggttc cgcagtcgtg gctggtttcg ggcttgaacg ctgtcacgct cacgtcgctg
     6601 aacggcgacg acgacatcag cggcatggcg aagtcccggc tcacgtatca gcatcttctc
     6661 cgcgccgata acggagcgct cgatgtgagt ctccccggcg gcagatccgt gacgatcggt
     6721 ggtttcggcg caaagacagt ccgtgccttc gacgtcacag atccgcggca cccgaccgag
     6781 ctccaggcca cggtcgctgc cgatccgcag ggcggtttcg cggctacgtt ctcgacgctg
     6841 gccggtggta tgcgcacagt gctcgccatc gacggcacgc gtttgcttgg gcatggagag
     6901 ctggccgcga acgcgccatc gtcgtggagc gcaagcagca acaacccaaa gagtccggcc
     6961 ttcttcatca tcagccacgg ttctttcctc ggcgccgccg cgacgttgaa ggccttgcgg
     7021 gacagccagg gaatcagcac cgcggtcgtc gacgtcgacg acctttacga cgaattcaat
     7081 ttcgggattc gctctccgga ggcgatccgt tccttcttcc gtgccgccgc cggttggaaa
     7141 cacccaccga gtgcggcact tcttcttggc gacgccagca tggatccgcg caactatctc
     7201 ggcctggcct cggtggactt cgtgccgacg aagctcgttc cgacgctcta catgaagacg
     7261 gcgtcggatg attggttcac agacttcaat ggcgacggca tcgcggacat cccggtgggc
     7321 cgcattccgg ttcgcactcc tcaggaagcg tcgctggtca tcggcaagat cacgtcgcgg
     7381 ggtacgccca gtggtgcatg gtcgaaaagc gcgctcttcg tggcggacag cccggtggat
     7441 ttcgactttc ccggtgtcgc ggcatcgctg acgacactac tgccgtcctc gatcacgagc
     7501 cagaccatcg acttctccag gacctcttcg ccgcacgcgg acgtcgtcaa tggcatgaac
     7561 aacggttcgc tgctcgtcac cta
//
LOCUS       DATLVW010000909         4387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_449663, whole genome shotgun sequence.
ACCESSION   DATLVW010000909 DATLVW010000000
VERSION     DATLVW010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449663"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VNN25_27000"
     CDS             <1..739
                     /locus_tag="VNN25_27000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95252.1"
                     /translation="ASVATKLDRYFCEGCGFRWSIVEDRPDAPEKPTERSKPSDELQL
                     PLKDILCGIADKLAALEKKLARVELLYESVDQPIGDLIIQLAEQCARIEAAQVKMLAR
                     VERSEQALVDLGDAVIDLINQVAADPAHLAGPWQERLDAIRQDLAKHSATAEITDPES
                     MSDEEILADVAASGLDPNAEASRVRKLMLDAAQKAMAEKRIERLERAFVQFAETPLAG
                     FSRDFLTALKAIRQDLAEIGWQRQPKE"
     gene            743..1030
                     /locus_tag="VNN25_27005"
     CDS             743..1030
                     /locus_tag="VNN25_27005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95253.1"
                     /translation="MTEENKTETNLEQARQLTVLDRHDAREDKRISLMERDVEQVLRI
                     REEQHQAWMQDVRHTKKHREEMQAIARDHAKSLASIAESLGTMAKAVAEKS"
     gene            1030..1392
                     /locus_tag="VNN25_27010"
     CDS             1030..1392
                     /locus_tag="VNN25_27010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95254.1"
                     /translation="MDIKLETDTIKAIVSEAVMASLTSEKREALIQNALNYLLTQKKD
                     GYSNHPPESLLESAFKQALGTIAREISSEMLANDTRVREVVRKMIADAVEKLLDPTTY
                     SETVERIKNAIEQGLSRR"
     gene            1389..1856
                     /locus_tag="VNN25_27015"
     CDS             1389..1856
                     /locus_tag="VNN25_27015"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HXH95255.1"
                     /translation="MSITWQRGDLLNDTADSLVNCVNCVGVMGAGIAAMFKLRFPSNY
                     EAYRYRCTHAPEKLLPGEIFYHRLADGRRIYNLATKGDFRDPSRLQWIREGLGRLAYR
                     VMDDGVQSIAVPALGCGRGGLAWSDVRPLIEHYLGDAMFQHVDVRVYEPMEGS"
     gene            1853..2155
                     /locus_tag="VNN25_27020"
     CDS             1853..2155
                     /locus_tag="VNN25_27020"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HXH95256.1"
                     /translation="MTVSRGWYRHYKGHAYVVLGVACLKHEGDRRVVIYTSVKTEEGS
                     EGFDFLARDEKDFEVWVDPENGQPFVGTQSESCFRRSGCVRRFERITDPEKSIVPA"
     gene            2152..2604
                     /locus_tag="VNN25_27025"
     CDS             2152..2604
                     /locus_tag="VNN25_27025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95257.1"
                     /translation="MISLDDKIRAQWERFLVDLPGFLLDDALAGKWICYLDGVHYAGA
                     DQNDAYRFGLERYGIDGGFVIAQVVEQKPLHVSRLGSAGWAIVQQQYDELKAKYDELQ
                     AENKRLCTIVAKARWFCEPRDAIRALLVINRHAFDELVAALDELEENQ"
     gene            2685..2942
                     /locus_tag="VNN25_27030"
     CDS             2685..2942
                     /locus_tag="VNN25_27030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95258.1"
                     /translation="MRSTKRIIAENAALLEKNVRLAAIVEAYRALRGVERELLQARAH
                     WDRVYYREEEPLVRQEATEAVRFLQERKQEIEAQLKKLEAT"
     gene            3035..3265
                     /locus_tag="VNN25_27035"
     CDS             3035..3265
                     /locus_tag="VNN25_27035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95259.1"
                     /translation="MREENTRLRAVIENLPVCAYGPCLNKGIWDFSDTEHGLRVYCQQ
                     HAEDRARDIGDFVEHCAELVSYADALGALETK"
     gene            3632..3988
                     /locus_tag="VNN25_27040"
     CDS             3632..3988
                     /locus_tag="VNN25_27040"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH95260.1"
                     /translation="MLNPEEPAIAGDDARLRAVTDAAVYAVLSGFCIDAEQELDESVR
                     EFERYDDPQRRLGRRLRALRKFCRKSMGDLAEATGETVVRVSDIERGIGEPAGDDFVT
                     KAAKYLGCIATFLEQP"
     gene            3985..>4387
                     /locus_tag="VNN25_27045"
     CDS             3985..>4387
                     /locus_tag="VNN25_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95261.1"
                     /translation="MNVQRYTLTLENSTTVTIELEHCGGTLWRHEILHGTPPFHYAFY
                     HTFGECLRVACLRVEERYGSPVVQCDRMFPLPVAEDQTFTEAELSYAQLRAAVAERLG
                     ELEAIVRAVAEMDPFCRDGDCVPCREWLGSAT"
BASE COUNT          967 a         1221 c         1410 g          789 t
ORIGIN      
        1 cgcaagcgtc gcaacgaaac tggaccggta cttctgcgaa ggttgtggct ttcgatggtc
       61 gattgtcgaa gaccgccccg acgctcccga gaagcccacc gagcgctcga agccaagcga
      121 cgagttgcaa cttccgctga aggacatcct gtgcggcatt gcagataagc ttgccgccct
      181 cgagaaaaag ctcgcgaggg tggagctgct gtacgaaagc gtggaccagc cgatcggcga
      241 cctcatcatt cagctggccg aacagtgcgc ccgaattgag gcagcgcagg tgaagatgct
      301 ggcaagagtg gagcgctcgg agcaagccct tgtggatcta ggtgatgcag tcatcgacct
      361 gatcaaccag gtcgcagccg accccgcgca cctcgcggga ccgtggcaag agcggctgga
      421 tgccatccgc caagacctcg ccaaacacag cgccacggcc gaaatcactg accccgagtc
      481 gatgagcgac gaggaaattc ttgcggacgt agcagcatca ggcctcgatc cgaacgcgga
      541 agcgagtcgc gtgcgtaagc ttatgctgga tgccgctcag aaggcgatgg ccgagaaacg
      601 catcgagcgg ctcgaacgag cgttcgtgca gttcgcagag acgccgctcg ctgggttcag
      661 ccgcgacttt ctgacagcac tgaaagccat ccgccaggac ctcgctgaga tcggctggca
      721 acgacaaccg aaggagtaga ccatgaccga agagaacaag accgaaacga acctcgagca
      781 agcccgtcaa ctcaccgtgc tagatcgcca cgatgcacgc gaggacaaac gcatctcgct
      841 catggagcga gacgtcgagc aagtcctacg cattcgcgag gagcagcacc aggcatggat
      901 gcaggatgtc cgccacacga aaaagcatcg ggaggagatg caggcgatcg cccgcgacca
      961 tgccaagagc ttggccagca tcgcggagtc gttagggacg atggcgaagg cagtggcgga
     1021 gaagtcgtaa tggacatcaa gctcgaaacc gacacgatta aggccatcgt cagcgaggct
     1081 gtcatggcct cgctcaccag cgaaaagcgt gaggcgttga tccagaacgc gctgaactat
     1141 ctgctaaccc agaagaagga cggctatagc aaccaccctc ccgaaagcct gctggagtcg
     1201 gcattcaagc aagcgcttgg taccattgcc cgagagatct ccagcgagat gctggcgaac
     1261 gatacccgtg tacgcgaggt ggtccgcaag atgatcgccg atgccgtcga gaagttgctg
     1321 gacccaacca cgtacagcga gacggtcgag cggatcaaga acgcgattga gcaaggtctt
     1381 agccggcgat gagcatcacg tggcaacgcg gggacctact gaacgacacg gcggacagcc
     1441 tcgtcaattg cgtcaattgc gtgggggtaa tgggcgccgg gatcgctgcg atgttcaagc
     1501 tcaggttccc gtcgaactat gaggcgtatc gatacaggtg cacgcacgcc ccggaaaagc
     1561 ttctgcccgg agagattttc taccatcgcc tcgcagacgg tcgacgcatc tacaacctgg
     1621 ccacgaaagg cgactttcgc gatccgtcca ggctgcaatg gattcgcgag gggctcggta
     1681 ggcttgcgta tcgcgtcatg gacgacggag tgcagtcgat tgcagtgccc gcgcttggct
     1741 gcggccgtgg cggtttagct tggtctgatg tgcgcccgct gatcgagcac taccttggtg
     1801 acgcaatgtt ccaacacgtc gacgttcgcg tttacgaacc catggaggga tcatgaccgt
     1861 ttcaagagga tggtaccgtc actacaaggg acacgcgtac gtggttttgg gcgtcgcttg
     1921 cttgaagcac gaaggcgatc gccgcgtggt gatctacacg tccgtgaaga ccgaagaggg
     1981 gagcgagggt ttcgactttc tcgcgcgaga cgagaaagat tttgaagtgt gggtggatcc
     2041 ggagaacggg caaccatttg tgggcacaca atccgagtcg tgttttaggc gatccggctg
     2101 cgtgcggcgg ttcgagcgca tcaccgatcc agagaagtcc atcgtgcccg catgattagc
     2161 ctcgacgaca agatccgcgc gcagtgggag cggtttctcg tggaccttcc tggattcctg
     2221 ctcgatgacg cgcttgcagg caagtggatt tgctacctgg acggcgtgca ttatgcgggc
     2281 gctgatcaga acgatgcata ccgatttggt ctggagcgct acggcatcga cggtgggttc
     2341 gtcatcgcgc aagtcgtcga gcagaagccg ctgcacgtgt cgcggctggg ctccgcagga
     2401 tgggcgattg ttcagcagca gtacgatgaa ctgaaagcga agtacgacga gcttcaggca
     2461 gagaacaagc gactgtgtac gatcgtggcc aaggcccgct ggttctgtga gccacgtgat
     2521 gccattcgcg ctttgctggt gatcaaccgg cacgcgtttg atgagctggt ggctgcgctg
     2581 gatgagctgg aggaaaatca gtgatccgct gctttagttg caacgcagag cttccaggcg
     2641 tggtgccatc ctgccaggta tgcgccgaga gagtggcttt gacgatgagg tctaccaagc
     2701 ggatcattgc cgagaacgct gcgctcctcg agaagaatgt gaggcttgcg gcaatcgtgg
     2761 aagcgtatcg ggctctgcgc ggcgttgagc gcgagctact ccaagcgcgg gcgcactggg
     2821 atcgagtgta ttaccgcgaa gaggaacctt tggtgcggca agaggcgacg gaagccgttc
     2881 gattcctgca ggaacgcaag caggagatcg aagcacagct caagaagctt gaggcaacat
     2941 gaagatgccg ggaccccagt ctaaaggcgt gtgtttcgcg tgtggattcg agcactggga
     3001 agactcgtgt ccggtgtgcg aaagaacgca gtctttgcgt gaagagaaca cccgcctgcg
     3061 agccgtgatc gaaaacttgc cggtatgcgc atacgggcct tgtctcaaca aaggcatctg
     3121 ggacttcagc gacaccgagc acggcctgcg tgtctactgc cagcagcatg ccgaagacag
     3181 agcgcgcgac attggcgact tcgtggagca ctgcgctgaa ctcgtttcgt atgccgatgc
     3241 ccttggcgcg ctggagacga agtgattacg tgtatccaag aagcctgggt ggagcagaac
     3301 ggcgaacact acatcgaatg ctactacttt tgtgatcaat gccgcggaac gaccatctca
     3361 ccggacccag cgccacactg ctccgtgagg tgccgtcagc cttcggatga attgacgcgc
     3421 ctacgcgccc tcgccgaagc cgccacgcgt ctggacatgc cccgattctg tgacgagcat
     3481 ggcccgaagg tccgcgagtg cccgcgtgct gtacccctac gggatacgat tgccgcgctg
     3541 agggcattgg aagggaagca tggcagttaa gtgcgggcta tgtggcggaa gctggccagc
     3601 gcagcgtgaa cgcgagctgg agaaggcacc cttgctgaac cctgaggagc cggcaatcgc
     3661 tggagatgac gcgaggctca gggcggtcac tgatgccgct gtgtatgcag tgctgtcagg
     3721 tttctgtatc gacgcagaac aggagctgga tgagtccgtg agggaattcg agcgttacga
     3781 cgatccacaa cgccgccttg gaagaagact gcgcgcgctg cggaagtttt gccgcaagag
     3841 catgggagac cttgcggaag ctaccggcga aaccgtcgtt cgcgtctctg acatcgagcg
     3901 cggcatcggc gaacctgcgg gtgatgactt cgtgacaaag gctgccaagt acctcggatg
     3961 catcgcgacg ttcttggagc aaccgtgaac gtccagcggt acaccctcac gctggagaac
     4021 agcacgaccg tcaccatcga gcttgagcac tgcggcggta cgctgtggcg acacgagatc
     4081 ttgcacggaa cgccaccgtt tcactacgcg ttctaccaca ccttcggcga gtgcctgcga
     4141 gtggcctgtc tgcgcgtcga ggaacgatat ggtagcccgg tcgtgcaatg cgaccggatg
     4201 ttcccactgc ctgtcgccga agaccagacg ttcacagagg ccgagctgag ctacgcccag
     4261 ctgagagccg cggtcgcgga acgccttggc gagctggagg caatcgtgcg ggcggtggca
     4321 gagatggatc cattctgccg agacggtgac tgtgtgccct gtcgcgaatg gctcggctca
     4381 gcaaccg
//
LOCUS       DATLVW010000910        12874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_164441, whole genome shotgun sequence.
ACCESSION   DATLVW010000910 DATLVW010000000
VERSION     DATLVW010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164441"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VNN25_27050"
     CDS             <1..1836
                     /locus_tag="VNN25_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95262.1"
                     /translation="VDDTRVVPLTKPTAVRASTFDEVESLVGGRESEKNLASNRDASS
                     PTPDSRQTTPADPTPDSRHPTPANPTPHSPPPTPPITDPQRHASAVLMWDLTLAPGAE
                     RIIDIATPLHAHDAQAIPADVAQQRASAIAYWRKIVDHVQIDIPAARDLVDTARANVA
                     YNLLGRDGPAIRGGARNYRRSWIRDGSLASTMLLRFGLDDDVRDYIRWFAPYQFADGK
                     IPCCVDSRGADPVAEHDSHGEFIYLVAEYARLTGDLTLAREIWPHVFAAGKYIDGLRG
                     VGSGGSGVGDKSAASTSTVSIESTVANPHAEPATTPHIPLPTPFTGILPPSISHEGYS
                     AKPMHSYWDDFFAMRGLKDATWLAEQLGERDAASRFAIVRDEFARDFHASIRNAIALH
                     HIDYIPGSADLGDFDPTSTTIGIEPGGDLANLPHDDVIDEFTRAWNESLSRMEGRRDW
                     KDYTPYELRQVGTFIHLGWRDRAQQLLQFFLDDRRPRGWNQWAEVVAQDYRAPTYLGD
                     IPHLWVGSDFARSFIDMLAYEREEDDALVLGAGIPDSWLERGVHVRGLRTIYGRLNFS
                     AQRQGGRVIVTISGVRVPKGGIVVMLPGSRERVVRKVPARIVLSQ"
     gene            2217..2561
                     /locus_tag="VNN25_27055"
     CDS             2217..2561
                     /locus_tag="VNN25_27055"
                     /inference="COORDINATES: protein motif:HMM:NF022671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HXH95263.1"
                     /translation="MAIPLDVRADAEIALAEFCKQNSSEAVADQSRYEFEILENHALL
                     VERRAGFLSHLEWTTVPVAKFRYSPAKFVWSLYWPDTNERWHRLSNSKAAKDIRTLLA
                     AVKSDTSGVFWE"
     gene            complement(2568..3362)
                     /locus_tag="VNN25_27060"
     CDS             complement(2568..3362)
                     /locus_tag="VNN25_27060"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXH95264.1"
                     /translation="MKLSRLGAVALALACTIPTAGAIELNCRGAANLESFRYSWHLRG
                     GLSWVAGIMFPTTGVGELKTTYQTAGPKTVDSSLLITAADGKSGFFAYETQMDAAGLR
                     TLMTYHAYSWKDKSRKDRTQFDYPAGVAHRHKETQEKAWDRNDPIPAGAFHDVVSVIH
                     FLRLSAGNIHGPLATTIYSDGKAYPVIVRPAERQTFSVAGQQIAAVGFELVDAPGGKK
                     WPGGMKIWLSDDARRIPFRIEIIQSMASLQLDLQSIDACSFLHAPK"
     gene            complement(3421..4263)
                     /gene="ispH"
                     /locus_tag="VNN25_27065"
     CDS             complement(3421..4263)
                     /gene="ispH"
                     /locus_tag="VNN25_27065"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXH95265.1"
                     /translation="MNVKRAAKYGFCSGVRIADLKVKRFAAGGGRGAILGQVVHNERV
                     VEEMEQLGVRTIDEIEEVAEPTIVFSAHGVPPSFHERATAAGLQVLDTTCKFVYDIHR
                     ESKQALANGYHLIFIGDPKHREVVGYTHDLDPASYHIVTKVDQADAIDWSQYPKLKVF
                     YQTTLNAEEFEDVVNAIERRAAQVERGDTICYATKENQDAARVLALDPEVDAIVVIGG
                     KMSANTKHLWDICREAKPSFLVQGASDLDPAWFANARTVGVTAGSSTPDYVIVEVEER
                     LSTM"
     gene            4676..5122
                     /locus_tag="VNN25_27070"
     CDS             4676..5122
                     /locus_tag="VNN25_27070"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH95266.1"
                     /translation="MDEVLIDTNILIYAHQPSETVKYDRALRAIETLTTSGLGRLSAQ
                     VLGEFMAATIRGRRSILGIDDALRQATLLASAFPVLDQTAMVVLEAGRGVREHQLSYF
                     DAQIWATARLNQVPTIFTEDFTNGRRLEGVHFLNPLLPSFDLERWS"
     gene            5179..5412
                     /locus_tag="VNN25_27075"
     CDS             5179..5412
                     /locus_tag="VNN25_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH95267.1"
                     /translation="MRITSKGQVTIPQEIRERFGLLPGTEVEFVANRHSVQIVKSKNP
                     ARPTDGEAMVRRLRGRSSVKMSTDEILALTRAS"
     gene            5493..6683
                     /locus_tag="VNN25_27080"
     CDS             5493..6683
                     /locus_tag="VNN25_27080"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH95268.1"
                     /translation="MTAAPAEPLFTPRFAMLWVYAFVTFFSAFQLLPAIPFRILELGG
                     SKAEAGWFLSVYTFSSAFAAPIMGTIADHLGRRRLLITASILFIAFSVAYGVVRNLPL
                     LFLIAAIHGAIWSGILASASALMSEYIPESRRTQGMAYWGLASTGAIAIAPAVGLWVY
                     HFGWMTLCFELAAISVVMVFGALLLRVDEKPASPRRLAVSDAWDWRVIATALSLTVAS
                     FGYGGMTSYAAIIAVERHITPVAIYLTTYAISIVLFRVCFSHLGDRIGPKRVLYPALV
                     LVPLAFAWLGIAHTRWEMIASATLFGIGFGAAYPAFVTIILGSTDPARRARTFGSIVW
                     AFDTGIGTGSFAIGAIGQHYGLTKAFLVAAALSCFSIPIFAWASARLTGNSFATEPEH
                     ARAN"
     gene            6667..6876
                     /locus_tag="VNN25_27085"
     CDS             6667..6876
                     /locus_tag="VNN25_27085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95269.1"
                     /translation="MPEPTEDERLRDRETVTTNAGDDDERVSRKVIYEHTATSGSSQS
                     ASITIIVIVVLALVLVGYILMHLHR"
     gene            6964..8016
                     /locus_tag="VNN25_27090"
     CDS             6964..8016
                     /locus_tag="VNN25_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HXH95270.1"
                     /translation="MIRLSIVALLLATTSLHAAEFDAKAAERFANLALACVHKEYPNK
                     IGHNLNSDNDVAPPRVLTPAFYGCYDWHSSVHGHWLLARLARTFPDAPFAGNAQAALK
                     QSLTPENIAQEAAYLRGEGRASFERPYGLAWLLQLAAELREWDDPLGRELSANLKPLE
                     QAANERLATWLPKLSHPVRSGEHSQSAFALGLIIDSTSDPALKQLARDAARRFFSTDK
                     ACPLTYEPSGEDFLSPCIAEGDVMRRVMPPKEYATWLRAFLPSLSSLRPEIVTDPSDP
                     KLAHLDGLNLSRAWMLEGIASGLPKSDPRVPLIRKIAAEHRDAGLASVTGEHYEGGHW
                     LGSFAVYLTTARGMKQ"
     gene            8084..8377
                     /locus_tag="VNN25_27095"
     CDS             8084..8377
                     /locus_tag="VNN25_27095"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXH95271.1"
                     /translation="MERSREETINVAEAKKQLSNLLGRVAFGRETILIMRRGRPMARL
                     VPPDEQPADQGLGAVRGWMSDDDPFLAEMEQIVSARVRHRPRTLRKPHGNGKR"
     gene            8374..8781
                     /locus_tag="VNN25_27100"
     CDS             8374..8781
                     /locus_tag="VNN25_27100"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXH95272.1"
                     /translation="MKYLLDTNVLSEVIRKDPNVGVLQRLNEVAPRDLVTSVVCVAEL
                     RHGAARVAHGARLWKRIRDEILSRVNVLPLGEAEAIRAGDLLATLEARGEPIGIEDVW
                     IGATALEYGLQVVTRNLKHFRRIPGLTSESWWT"
     gene            8895..9146
                     /gene="vapB"
                     /locus_tag="VNN25_27105"
     CDS             8895..9146
                     /gene="vapB"
                     /locus_tag="VNN25_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017995839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HXH95273.1"
                     /translation="MQIAKVFWSGRSQAVRLPKRFRLESREVQISKRGNEIILKPLPD
                     TWAWLDEIAGPLDPDFVEAAQEQPQTTIGQLSRKSSGRK"
     gene            complement(9164..10618)
                     /locus_tag="VNN25_27110"
     CDS             complement(9164..10618)
                     /locus_tag="VNN25_27110"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH95274.1"
                     /translation="MKNVLAIATAMFVMQTAAIAQVETTPPPPAAPREVQIPKPAEKT
                     LANGLRVIVIEKQGVPLVAARVLIKNGGEADPAALPGLADTTASLLTQGTATRSAEEI
                     ARGVEALGATLDTGAGWDQSSADISVMSSKLPQALAFMADVLLNPAFKQEEIDRLRKR
                     NSDALAVAMKQPGRLASFVATHVIFGDAPYGHNLGGTPQSLARITRDDIVAFHRDYYR
                     PDNAILIMGGDITSAKAFAVAQELFGEWKPATGSHLAETTSPKALPPSRVVVVDMPSA
                     GQAAVVVSRPGMRRADPAYYLALVTNSILGGGFSSRLNQEIRIKRGLSYGAGSSFDLR
                     RDTGPFTATAQTKNESAGEVAGLVIDEMNKLGTTVVDPTELGPRKAVLIGSFGRQLET
                     NAGLVNRLGTLALYGLNLDEVNRYISGVQAVSADDVEKFAASHVPGNDASIVIVGDAK
                     KFLPALQARFKTIDVIPIDDLDVSAATLRKGKTP"
     gene            complement(10615..11964)
                     /locus_tag="VNN25_27115"
     CDS             complement(10615..11964)
                     /locus_tag="VNN25_27115"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXH95275.1"
                     /translation="MSKPRRLAEFLIVLALASNAFAATPAKSPAVAPINYQHRSLANG
                     LEVYSIENHATPTVSIQVWYRVGSKNDPEGRSGFAHLFEHIMFKATAHMKSEMLDRLT
                     EDVGGMNNAYTHPDRTVYYEIVPSNYLQTLLWAEGERLGSLKIDEDTFKSERDVVKEE
                     YRTRVLAPSYGRLNYAITKDSFAVHPYRRPGIGSIEELDAATVPDVTAFHATYYRPDN
                     AVVIVAGDFDPKQLDTWVDRYLGTIPKPSTAIPRVTVQEPARTEEKHFAEHGANVPLP
                     AGALTWLIPSARSDDAEPLEIAATILSDGDSSRLYQSLVYRKQLAQSAGASANLQEDV
                     SLFTARAIMASGKKPEDGIAALREEIAALAAKQVTAAELEKAKNLIITSALRERETND
                     GKAGALGEAITQNHDATYVNRGLARLQAVTAADVQRVVKKYLVDGKAVVITYTSEAK"
     gene            complement(12004..>12874)
                     /locus_tag="VNN25_27120"
     CDS             complement(12004..>12874)
                     /locus_tag="VNN25_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95276.1"
                     /translation="AVLAAVGLSILMLPNLQALPYFGLVLPPAPPTPPTPPTPPATPA
                     MSSIDVVPEPPEAPEPPTPPEPVEMASMDVEVPEAPEPPEPPEPPAAWHERDGDAKYV
                     LSFGDMNVVNGDGYTNFEHLRHAYGRNFFWFRHEGTSYIVRDPEMVRELAELYRPQSE
                     LGREQGALGRKQGALGRQQGELGRRQGELGREQARIGVEQARMSRRYDDDDDPSFSAR
                     RRELERKERDLSRRQDELGREQSALGREQSKLGEEQSRLGERQSELSREIEHKLQPMI
                     REMIRKGVAERVR"
BASE COUNT         2334 a         4045 c         4020 g         2475 t
ORIGIN      
        1 gtcgacgaca cgcgcgttgt tccgctgacg aaaccgacgg cggtcagggc gtcgacgttt
       61 gatgaggtgg agtcgctggt cgggggtcgg gagtcggaga aaaacctggc gtcaaatcgg
      121 gacgcctcat ctccgacccc cgactcccga caaacgacac ccgcggatcc gacccccgac
      181 tcccgacacc cgactcccgc gaatcccacc ccccactccc caccccccac accccccatc
      241 accgatcccc aacgccacgc ctccgccgtc ctgatgtggg acctcactct cgcgcccggc
      301 gcggagcgca tcatcgacat cgccacgccg cttcacgcgc acgacgcaca agcgattccg
      361 gccgacgtcg cgcaacagcg cgcgagcgcg atcgcttatt ggcgcaagat cgtcgatcac
      421 gttcagatcg acatccccgc cgcgcgcgat ctcgtcgaca ccgcgcgcgc caacgtcgcc
      481 tacaacctcc tcgggcgcga tggtccggcc atccgcggcg gcgcgcgcaa ctaccgccgg
      541 agctggatcc gcgacggctc tctcgcctcg acgatgctgc tgcgtttcgg gctcgacgac
      601 gacgtccgcg actacatccg ctggttcgcg ccatatcaat tcgcagacgg caagatcccc
      661 tgctgcgtgg acagccgagg agccgatccg gtggccgagc acgacagcca cggcgaattc
      721 atctacctcg tcgccgagta cgcgagactg accggcgacc tcacgctcgc gcgcgagatc
      781 tggccgcatg tgtttgcggc agggaagtac atcgatggac tgcggggggt ggggagtggg
      841 gggtcggggg tgggagataa atccgcggcg agcacgagca cggtgagcat tgaatcaacg
      901 gtcgcaaatc cgcacgccga gcccgctacc accccccaca tcccactccc cactcccttc
      961 acaggcatcc ttcccccatc catcagccac gaaggctact ccgcgaagcc gatgcattcg
     1021 tactgggacg atttctttgc gatgcgcggc ctcaaagacg cgacctggct cgcggagcaa
     1081 ctcggcgagc gcgacgcggc atcgcgtttc gctatcgtgc gcgatgagtt tgcccgcgat
     1141 tttcacgcgt cgatccgcaa cgccatcgcg ctgcatcaca tcgactacat ccccggctcc
     1201 gccgacctcg gcgatttcga tccgacgtcg accacgatcg gcatcgagcc cggcggcgac
     1261 ctcgccaatc tgccgcacga cgacgtcatc gacgagttca cgcgcgcatg gaacgagtcg
     1321 ctctcgcgca tggaaggccg ccgtgattgg aaggactaca cgccatacga gctccgccag
     1381 gtcggcacgt tcatccacct cggatggcgc gaccgcgcac agcaactcct ccaattcttt
     1441 ctcgacgacc gccgcccgcg cggatggaat cagtgggccg aggtcgtagc gcaggattac
     1501 cgcgcgccaa cgtacctcgg ggacatcccc cacctctggg tcggctccga cttcgcccgc
     1561 tccttcatcg acatgctcgc ctacgagcgc gaagaagacg atgcgctcgt cctcggcgcc
     1621 ggcatccccg actcatggct cgagcgcggc gttcacgtac gcggacttcg cacgatctac
     1681 ggccggctca atttctcggc gcagcgccag ggagggcggg tcatcgtgac aatcagcggc
     1741 gtgcgcgtgc cgaagggtgg gatcgtggta atgctgccgg gatctcgcga gcgcgtcgtt
     1801 cgaaaagtgc cggcgcgaat cgtattgtct caatagcgag ttgttgggcg tgaatttgat
     1861 gtgtcatcct gaggctgccg gagccgccat ccgtactcga acggtcgcgc tgcaagatgt
     1921 ggcggcggag gacgccgaag gatctcaagt tacgacgcat atgccacacg cctcgtattc
     1981 cgagatcctt cgccgtcctg cggcgccatc tcatcatgcg tgaaaggcgc gagcagcgcg
     2041 ggcgcctccg gcaggctcag gatgacacat cacgtttagt cgcagcgcgc ttcttcctgg
     2101 gtcgcggtgc ttcagccagg gcagcgatga cagcccgaag caactggttg actgactccg
     2161 aattgcggaa gaccgacgca acatcaggat cgaggccgca tgagttacga ttgggtgtgg
     2221 caattcctct tgacgttcgc gctgatgccg aaattgccct cgccgagttt tgcaaacaga
     2281 actcgagtga ggcggtggct gatcagtcgc gctacgagtt tgagattctc gaaaaccacg
     2341 cgctcctggt cgagcggcgg gctggctttc tgagccatct cgaatggact accgtcccgg
     2401 tggcgaagtt ccgctactcg ccggccaagt tcgtctggtc gctctactgg cccgacacga
     2461 acgagcggtg gcacaggctg tcgaattcga aggcggcgaa ggacattcgc acgctgctgg
     2521 cggccgtgaa gtcggatacc tccggcgtgt tctgggagta gatccgacta cttcggcgcg
     2581 tgcagaaacg agcaggcatc gatcgactga agatcgagct gcagcgacgc catgctctgg
     2641 atgatctcga tgcggaacgg aatgcgccgc gcgtcatcgg aaagccagat cttcattccg
     2701 ccgggccact tctttccgcc gggagcatcg accagctcga atccgaccgc cgcgatctgc
     2761 tgtcctgcga cggaaaacgt ttgccgctcc gccggccgga cgatcaccgg gtacgccttc
     2821 ccatccgagt agatcgtcgt ggcaagagga ccgtggatgt tgccggcgct caatcgcagg
     2881 aagtgaatca cgctcacaac gtcatgaaac gcgccggccg gaatcggatc gttgcgatcc
     2941 catgccttct cctgcgtctc tttgtgccgg tgcgcgacgc cggccggata atcgaattgc
     3001 gttcggtcct tgcgtgattt gtccttccac gaatacgcgt gatacgtcat cagcgtccgc
     3061 aaaccggccg catccatctg cgtctcgtag gcgaagaaac cgcttttccc atccgcggcg
     3121 gtgatgagga gcgagctgtc gaccgtcttc gggcccgcgg tctggtacgt cgtcttgagc
     3181 tcgccgacgc ccgtggtggg aaacatgatc ccggcgaccc aggagagtcc gccgcgaaga
     3241 tgccaggagt agcgaaacga ctcgagattc gcagcgccgc ggcaattgag ctcgatcgct
     3301 cccgccgtgg gaatcgtgca ggctagcgcg agtgctacgg ccccgaggcg agagagcttc
     3361 atgaacgcga gggaaaacac gccacagaag gttgctattg ctggctttgt gccggagccg
     3421 ctacatcgtg ctcagccgtt cctcgacctc gacgatcaca tagtcgggag tcgacgagcc
     3481 ggcggtgacg cctaccgttc gtgcattcgc aaaccatgcc ggatcgagat cgctcgctcc
     3541 ctgcacgagg aacgaaggtt tggcctcccg gcaaatgtcc catagatgtt tcgtgttcgc
     3601 gctcatcttt ccgccgatga cgacgatggc atcgacttcg ggatcgagag ccagcacgcg
     3661 cgccgcatcc tgattctctt tcgtggcgta gcagatcgta tcgccgcgct ccacctgagc
     3721 ggcccggcgc tcgatcgcgt taacgacgtc ctcaaactct tcggcattga gcgtggtctg
     3781 gtagaaaacc ttcagtttcg gatactgcga ccaatcgatg gcatcggcct gatcaacctt
     3841 tgtcacgatg tggtaggacg cgggatcgag gtcgtgcgtg tatccgacga cttcacggtg
     3901 cttcggatcg ccgatgaaga tgaggtggta gccgttggcg agagcctgct tcgattcacg
     3961 atggatgtcg tatacaaact tgcaggtcgt atcgagcacc tgcaagcccg ccgccgtggc
     4021 acgctcgtga aacgacggcg gcacgccatg agcggaaaag acgatggtcg gctccgcaac
     4081 ttcttcgatc tcgtcgatcg tccgcacgcc gagctgttcc atctcttcga ccacgcgctc
     4141 attgtgaacg acctggccga ggatggcgcc gcggccgccg ccggcggcaa agcgtttcac
     4201 cttcagatca gcgatccgga caccggaaca gaagccgtac ttggccgcgc gtttgacatt
     4261 catcggaggt agggaacgca tcgaatgggt gggacattct ggggagggaa ccgacgatgc
     4321 cgatcgagag gaagtaccct caggaagaac ggtgttgatg gcaacggttg cggccatgat
     4381 gtatattgtg atgtatgaag gctggctcaa tgatacgaaa gcaggtctac atcgaagccc
     4441 gtcatgatcg aatgctgaaa cgacgggcca gtcaacgcgg cgtaaccgaa gcggagctca
     4501 ttcgggaggc cctcgatggt gtggaggtcg gtgctgtcgt ccgacgccgt gagcctgatg
     4561 cggctgccgc ccgaaaggct atcgcattca tgcgctccct ctcggcctca cgcggaggat
     4621 cgccctcgga tcggaagtgg tcgagagact cgctttatga agatcggatc ggtcgatgga
     4681 cgaagtcctg atcgatacga acattctcat ctacgcacac cagccatcgg agaccgtaaa
     4741 gtacgacaga gcgttacgcg cgatcgagac tctgacgaca agcggacttg gacggctaag
     4801 cgcgcaggtg ttgggggagt ttatggccgc gacgatccgc ggtcgccgct ccatccttgg
     4861 aatcgacgac gccttgaggc aggcgacatt gctcgccagc gcttttcccg ttcttgatca
     4921 gaccgcaatg gttgttctgg aagccggccg aggagttcgg gagcatcaac tcagctactt
     4981 cgacgcacag atctgggcca cagcacgcct aaatcaggtg ccaaccatct tcaccgagga
     5041 cttcacaaac ggacgccgcc tcgaaggcgt gcacttcctg aatccgcttc tgccctcgtt
     5101 cgacctcgaa cgatggtcgt gaagaatcct ctcgctggca gataattctt accggtggag
     5161 gtaagaaaaa atacgcccat gcgaatcact tcgaagggac aggtgaccat cccgcaggag
     5221 atccgggagc gattcggact tctgccgggc acggaagtgg agttcgtcgc gaacaggcac
     5281 tctgttcaaa tcgtgaaatc gaagaacccg gcgcggccaa cagacggcga agcgatggtt
     5341 cgccgtctgc gaggccgttc aagcgtaaag atgtcgaccg acgagatcct ggcactgaca
     5401 cgggcttcct gaatgtatac agtcattgcc accagcctcc cgcatcagag gcagccggac
     5461 gcatcggccc ggcgaataca atccgccttc cgatgaccgc cgccccagcc gaaccgctct
     5521 tcacgccccg ctttgcgatg ttgtgggtgt acgcgttcgt cacgttcttc tccgcgtttc
     5581 agcttcttcc cgccattccg tttcggatcc tggaactggg cggctcgaaa gcggaggcgg
     5641 gatggttcct ctccgtctac accttctcca gcgcatttgc cgcgccgatc atgggaacca
     5701 tcgccgatca cctcggacgg cggcggctgc tcatcaccgc gtcgattctc ttcatcgcgt
     5761 tttccgtcgc gtacggtgtg gtgcgcaacc ttccgctcct ctttctcatc gccgctattc
     5821 acggtgcgat ctggtcgggg atcctcgcct cggcatcggc actcatgagc gagtacattc
     5881 cggagtcgcg gcgcacgcag gggatggcgt actggggtct ggccagcacc ggcgcgatcg
     5941 ccatcgcacc cgcggtcggg ctctgggtct accacttcgg ctggatgaca ctctgcttcg
     6001 agctggccgc catctcggtg gtgatggtgt tcggcgcgct ccttctgcgt gtcgacgaaa
     6061 aaccagcgag cccgcgccgc ctcgccgtgt ccgacgcctg ggactggcgc gtcatcgcca
     6121 ccgcgctctc gctgaccgtg gcatcgttcg gatacggcgg gatgaccagc tacgcagcga
     6181 tcatcgccgt cgagcgccac atcaccccgg tagcgatcta tctgacgacg tacgcgatct
     6241 cgatcgttct ctttcgcgtc tgtttctcac acctcggcga ccgcatcgga ccgaagcgag
     6301 tgctgtatcc ggcactcgtg ctcgtgcctc tcgcattcgc atggctcggc atcgcgcaca
     6361 cacgctggga gatgatcgcc tcggccacgc tcttcggcat cggcttcggc gctgcctacc
     6421 cggcattcgt cacgatcatc ctcggcagca ccgacccggc gcggcgcgca cgcacgttcg
     6481 gctcaatcgt ctgggcattc gataccggca tcggaacggg ttcattcgca atcggcgcga
     6541 tcggccaaca ttacggcctg acgaaggcgt ttctcgttgc tgcggcgctg agttgcttct
     6601 ccattccgat cttcgcctgg gcctcggcgc gtctcactgg caattcattt gctactgaac
     6661 ccgagcatgc ccgagccaac tgaagacgaa cgcctccgcg accgcgaaac agtcaccacc
     6721 aacgccggcg acgacgacga gcgcgtctcg cgcaaagtga tctacgagca caccgcgacg
     6781 agcggctcat cacaatcggc ttcgatcacg atcatcgtca tcgtggtgct ggcgctggtg
     6841 ctggttgggt acatcctgat gcacttgcat cggtgatgat tcaatcgccg tcgagccgac
     6901 ggcggaccag ccggcgggcc gccggcgcta cgagtgtggg gcgccacgca ttaccattcg
     6961 cgcatgattc gactttcgat cgttgcactg cttctcgcca ccacctccct ccacgccgcc
     7021 gaatttgatg caaaggcggc cgaacgattt gcgaaccttg cgctggcgtg cgtgcacaag
     7081 gagtatccga acaagatcgg gcacaacttg aacagcgata atgacgtcgc gccgccgcgg
     7141 gtgttgactc cggcgttcta cggttgctac gactggcact cgtcggttca tggtcactgg
     7201 ttgctggcgc ggctggcgcg aacgttcccg gacgcgccgt ttgcggggaa cgcgcaagca
     7261 gctctgaaac agagcctgac gccggagaac atcgcgcagg aggcggcgta tctgcgcggc
     7321 gaggggcggg cgagtttcga gcggccgtat gggctggcgt ggctgctcca actggccgcg
     7381 gagttgcgcg aatgggatga cccgctcggg cgcgaattgt ccgcgaatct gaaaccgctc
     7441 gagcaagcgg cgaatgagcg gctggcgaca tggttgccga aactctcgca tccggtgcgc
     7501 tccggcgaac atagtcagtc tgccttcgcg ctcgggctca tcatcgattc gacgagcgat
     7561 cccgcactga aacaactcgc tcgcgacgcc gcgcgacgct tcttctcgac cgacaaagca
     7621 tgcccgctga cctacgagcc ctcgggcgaa gattttctct caccgtgcat cgccgaaggc
     7681 gacgtgatgc gccgcgtcat gccgccgaag gagtacgcga cgtggctgcg cgcatttctg
     7741 ccgagtctct catcgctgcg tccggagatc gtgacggacc cgagcgatcc gaaactcgca
     7801 cacctcgacg gactgaatct cagccgcgcc tggatgctgg aaggaatcgc ctcaggattg
     7861 ccgaagtccg atccgcgcgt tcctctgatc cgcaaaattg cagccgagca tcgcgatgcc
     7921 ggtctggcgt cggtcacggg agagcattac gaaggtgggc attggttagg gtcgttcgcg
     7981 gtgtatctga cgacggcacg agggatgaag cagtagtagc gtcttgttga ccttcttgac
     8041 ctagatgact atctggatca atcatggcct ggaagaggag ccaatggaaa gatcacgcga
     8101 agaaacgatc aacgtcgccg aggcaaagaa gcagctttcg aacttgctgg gacgcgtggc
     8161 gtttggcagg gaaacgattc tgatcatgag gcgaggccgt ccgatggcga gactcgttcc
     8221 tccggatgag caacctgctg accaggggct cggtgcggtg cggggatgga tgtccgacga
     8281 tgatcctttc ttggctgaga tggagcagat tgtctccgcg cgcgtccgac accgtcctcg
     8341 gacgttgcgc aaaccgcacg ggaatggcaa gcggtgaagt atctccttga caccaatgta
     8401 ctttctgagg tgatccgcaa agatccgaac gtaggggttt tacagcgatt aaacgaagtt
     8461 gcgccgcgag acttggtgac ttcggtggtc tgcgtagccg agcttcgtca cggagccgcc
     8521 cgcgttgctc atggcgcgcg cctttggaaa aggattcgtg atgagattct gagccgcgtc
     8581 aacgtgctcc ctctgggcga ggcggaggcc attcgtgccg gagacctcct cgccacactc
     8641 gaagcacgag gcgagccgat cggcatcgaa gacgtatgga tcggcgcgac ggccctcgaa
     8701 tatggattac aggtggtgac gcgaaatctg aagcatttca ggagaatccc tggcctcaca
     8761 tccgagtcgt ggtggacctg accgttgaga gctgcggcga tcgaggctcg tcctacgtca
     8821 acggcgcata cgttcggcca tgttccgcgg attgacgtcg cgctacctct cattcgcatc
     8881 tcacggaggc tgacatgcaa attgccaaag tattctggtc gggccgctcg caggcggtga
     8941 gactgcccaa gcgattccgt ttggaatctc gagaagtgca gatcagcaaa cgcgggaacg
     9001 agatcatcct caaaccactg ccagacacct gggcgtggct cgacgagatc gcagggccac
     9061 tggatccaga ttttgtcgag gccgctcaag agcagccgca gacaaccatc ggccagctct
     9121 cgcgaaagtc ttcaggtcgt aagtgacttg ctgaagacgc gcgttacggc gtcttcccct
     9181 tccgcagcgt cgccgcgctc acatccaaat cgtcgattgg gatcacgtcg atggtcttga
     9241 aacgcgcctg caacgctggc aggaacttct tcgcgtcgcc gacgatgacg atgctggcgt
     9301 cgttgccggg gacgtgggag gcggcgaact tctccacatc gtcggcgctt acggcctgta
     9361 cgccgctgat gtagcgattg acctcatcga ggttcaatcc gtaaagcgcc agggtgccga
     9421 ggcggttgac gagaccggcg ttcgtctcca gttggcggcc gaaacttccg atgagcacgg
     9481 cctttcgcgg accgagctcc gtcggatcga cgaccgtcgt gccgagcttg ttcatctcgt
     9541 cgatcaccaa tcccgccacc tcgcccgccg attcattctt cgtctgcgcg gtggcggtaa
     9601 acggcccggt atcgcggcga agatcgaagg agctgcccgc gccgtagctc aggccgcgct
     9661 tgatgcggat ctcttgattg agccgcgaag aaaagccgcc gccgaggatt gaattcgtga
     9721 cgagcgcgag gtaatacgcg ggatcggccc ggcgcatccc cgggcggctg acaacgactg
     9781 ctgcctgtcc ggccgagggc atgtccacaa caacgacgcg cgacggcgga agagctttcg
     9841 gcgaagtcgt ctccgccagg tgcgagccgg ttgccggctt ccattcgccg aacaactcct
     9901 gcgccaccgc gaatgctttc gccgaggtga tgtcgccgcc catgatgagg atggcgttgt
     9961 cgggacggta gtagtcgcgg tgaaacgcga cgatgtcgtc gcgcgtgatg cgggcgagtg
    10021 attgcggcgt accgccgagg ttgtgaccgt agggcgcgtc gccgaagatc acgtgcgtag
    10081 ccacaaagct ggccagccgg ccgggctgct tcatcgccac agccagggcg tcgctgttcc
    10141 gtttgcgcag gcggtcgatc tcttcctgct tgaacgccgg attgaggagg acatcggcca
    10201 tgaaggccag cgcctgcggc agcttcgacg acatcacgct gatgtcggcc gacgattgat
    10261 cccaccctgc gcccgtgtcg agtgtggcgc cgagcgcttc cacgccgcgc gcaatctctt
    10321 ccgcgctccg ggtggccgtc ccctgcgtga ggagggatgc agtcgtgtcg gcgagaccgg
    10381 gcagtgcggc gggatcggct tcgccgccgt tcttgatcag cacgcgcgcg gcgacgagcg
    10441 gcacgccctg tttctcgatg acgatgacgc gcaatccgtt ggcgagcgtc ttctcggccg
    10501 gcttcgggat ctgcacttcg cgcggtgcgg cgggcggcgg aggcgtggtc tcgacctgcg
    10561 caatggcagc ggtctgcatc acgaacatcg cagtggcgat agcaagcacg ttcttcattt
    10621 cgcctccgag gtgtacgtga tgacgacggc cttgccgtcg accagatact tcttgacgac
    10681 gcgttgcacg tcggcggcgg tcacagcctg caaacgcgcc agaccgcggt tcacgtaagt
    10741 agcatcgtga ttttgcgtga tcgcttcacc gagcgcgccc gctttgccgt cgttcgtttc
    10801 gcgctcgcgc agtgccgagg tgatgatgag gttcttcgcc ttctccagct ccgcggcggt
    10861 gacctgtttc gcggccagcg cggcaatctc ttcgcgcagc gcggcaatac catcctccgg
    10921 cttctttccg ctggccatga tcgctctggc agtgaagagg ctgacgtcct cctgcagatt
    10981 ggccgaggct cccgcgctct gcgccaactg tttccgatag accagcgatt gatagagccg
    11041 cgatgagtcg ccgtcggaga ggattgtcgc ggcgatctcc agcggctcgg cgtcgtcgct
    11101 tcgggccgat ggaatgagcc acgtcagcgc tccggcgggg agcggcacgt tcgcgccgtg
    11161 ctcggcgaag tgcttctctt cggtgcgcgc cggctcctgc accgtgacgc gaggaatcgc
    11221 cgtcgatggc ttcgggatcg tgcctagata gcggtccacc caggtgtcga gttgcttggg
    11281 atcgaagtcg ccggcgacga tgacgacggc gttgtcgggc cggtagtacg tcgcgtggaa
    11341 cgcagtgacg tcggggacgg tcgccgcgtc gagctcctcg atcgagccga tgcccgggcg
    11401 ccggtacgga tggacagcga aggagtcttt cgtgattgcg tagttcaacc ggccgtacga
    11461 cggtgccagg acgcgtgtcc ggtattcttc tttgacgacg tcgcgctccg acttgaacgt
    11521 gtcttcgtcg atcttgagcg atccgagccg ctcgccctcc gcccacagca gcgtctgcag
    11581 gtagttcgac ggaacgatct cgtagtagac cgtccggtcg ggatgcgtgt acgcgttgtt
    11641 catcccgccg acatcttcgg tgagccggtc gagcatctcc gacttcatgt gcgcggtcgc
    11701 cttgaacatg atgtgctcga agagatgcgc gaaaccgctg cgcccttccg gatcgttctt
    11761 cgagccgacg cgataccaga cctgaatcga gacggtcggc gtggcgtgat tctcgatcga
    11821 gtagacctcg agaccgttcg cgagcgagcg gtgctggtag ttgatcggtg cgacggccgg
    11881 gctctttgcc ggagtggccg cgaatgcgtt tgaggcgagc gcgagaacga tcagaaactc
    11941 tgcgagacgg cgcggcttgc tcataggggg cggactatat caacgtgagc agcagtactt
    12001 tcatcagcgc acccgctcgg cgacgccctt gcgaatcatc tcgcggatca tcggctgcag
    12061 tttgtgctcg atctcgcggc tcagctccga ctgacgctcg ccgagtcggg actgctcttc
    12121 gccgagtttg gactgttcgc gaccgagcgc cgactgctcg cgtccgagct cgtcctgcct
    12181 gcgcgagaga tcgcgttcct tccgttccag ttcgcggcgg cgtgcggaga acgacgggtc
    12241 atcgtcatcg tcgtatcgcc gggacatgcg cgcctgttcg acgccgatac gagcttgctc
    12301 gcgccccagt tcgccttgcc tgcgcccgag ctcaccctgc tgccgtccga gcgcaccttg
    12361 cttgcggcca agcgcgccct gttcgcggcc cagctcactc tgcggacgat agagctcagc
    12421 aagctcgcgc accatttccg ggtcgcggac gatgtacgac gttccctcgt gccggaacca
    12481 gaagaagttg cggccatagg catggcgaag atgttcgaaa ttggtgtagc cgtcgccgtt
    12541 gacgacgttc atgtcgccga aggagaggac gtatttggcg tcgccatcgc gttcgtgcca
    12601 cgcggcgggg ggctccggcg gctcaggtgg ttcgggtgct tcgggcacct ctacatccat
    12661 tgaggccatt tccaccggct cgggaggagt gggcggctcc ggcgcttccg gcggctccgg
    12721 cacgacatcg atcgacgaca tcgccggagt tgcaggcggt gtcggaggtg tgggaggcgt
    12781 cggtggggcg ggaggcagga ccagcccgaa gtacggaaga gcctgcaggt taggcaacat
    12841 gaggattgac aagccaacag ccgcgaggac ggcc
//
LOCUS       DATLVW010000911         8293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_328233, whole genome shotgun sequence.
ACCESSION   DATLVW010000911 DATLVW010000000
VERSION     DATLVW010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328233"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VNN25_27125"
     CDS             <1..937
                     /locus_tag="VNN25_27125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95277.1"
                     /translation="REDRERAFAIFTELVHIGRESIVISALGDRGADEVWETAFPIDA
                     CEPSADLLASIASFWEETIDPGTTHGALRLVATLIRHGVSETPAAMRAHVDAVRSLAA
                     SASAPGWHPQPGDASLQNAAGQGAPHSNALLIAIGRSRPELVTSVASTVRAAAIATNT
                     MIGSAFAGYALLLPIIAELEPPLERAARELVLAKCAAADDVFLDAILQLVQGDDVEDE
                     TTRSDDELETIANEVLASFGNRIPGFARSSAAHLRENFLDGTGAIRFERERIEILLPH
                     VPLALLLRMFGVHGKTFALPWLPERTITLLMPEDA"
     gene            934..3624
                     /locus_tag="VNN25_27130"
     CDS             934..3624
                     /locus_tag="VNN25_27130"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HXH95278.1"
                     /translation="MSERKSEHSATPEASPEPRRSSTSTQPLQMLLRRAGNNAVCGIV
                     QAKLRVGGASVPLEEEAERAASTLGGGCCAAKPEGEQCDECNVRRKAIAPDQASADAP
                     PSVDRALHGSGIPLDDATRTSMESHFDSDLSDVRVHTGGDASRSADEIGAAAYTSGRD
                     IVFSNDGYQPHSGDGRRLLAHELTHVLQQRGRTDRTVRRQPAKPIPTKAVDDPCSISA
                     TSLTNAELISVFANTRNYVKTHKRGEDQLWYDHGNLLRRLASEVDRRRKLRHVWLGDE
                     SLTSMPGVLYELRGGMGNAIQIYPADVKAEAGAPYGDGRASVMTTGQFNAYLEKQQIK
                     QIDAQEYFAKQDPKNIEPLTYMLPEPQVPKWAQGQQIWGPAASLLFSNPMSYGANPFN
                     PIGMAGSGWGMTELTAFGGYPNPFGTFASKSASQGIYKNNANPATLSVREQSGGEVNW
                     RGDIPEITTARSSWADVVIYSDLNKVKANFEVFDYRNRFSGDLVSVTHTLPDRKGKTS
                     FNQYDTKFLRAINADQAAAEQAKFGRALQQLNQKYGTSTQVIDANEKTYLAINEDHVT
                     GYRTQLAADVVADPGSYAPLLDALLRKDPVTFQNADGTRTTISTWNDLQNARATNAIP
                     AGDFDMAIGRLSTATSQRVISNGITTTQLQVMQKFRAQNFGVPQETWSRSVAPELLDA
                     MRYGEINPTTGLAEPNYAPMVKSAAKGGAVMGAGFSLLTDAVRFGRNPGQHPDMWREG
                     LVDVGLGGLGGAGSASVESYVAARTSAALMGETGGGAAAMRILGRAGGGALGSLAAPV
                     VTMASMALSSQDYTSLEYYGALGRSSATGAVGGIGGALATGTYFAIAGSEFPLVGNAI
                     GFVVGFGGALIFDAIWGEDIDRGIREAGGELGCVDKAPPK"
     gene            3621..5714
                     /locus_tag="VNN25_27135"
     CDS             3621..5714
                     /locus_tag="VNN25_27135"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH95279.1"
                     /translation="MNVHLLVAAAPFADARAHLDAELALLDLRLRRQVMRLRAARLLT
                     EDQFRGLYIPDDQADAILQRAENEDVTAAALTRHIDELRGAIDRRAALTVRGGKTLPL
                     VALRERFGLTAFEIEILIIAVAPEIDLRYHTLYAYAQNDMTRRYPTVDFALQLLFASR
                     EDRHEHRAAFRPDAPLMQHRLVRLGEDAQEKEQSLLARPLRTDERVTDQILGGDALDP
                     MLVAAARSVTPRKRLDDLVLAAAVREQVLNVIPLLARGGAALVLEGARGTGRKSIAEA
                     ICGAMNAGMIVVDGARAATLPDAAALLRREALLSGRALVIDDFEKLPAGSRFDGAIRF
                     LITTEPVSADGYRIDVPMPGAAMRARLWRDAGAAPADDALAAKFVLSGGQIRVAVEHA
                     RAAAALRGAPGIELRDLIAGAHAQSSASLRALAQKVGLHFGWSDIVLPAKPLQQLREI
                     CISVQYRRVVFDDWGFEGRVAGGKGVAVLFNGASGTGKTMAASIIARELGLQMYRVDL
                     STVVSKYIGETEKNLSRIFAEAQASSAVLFFDEADALFGKRSEVKDAHDRYANIEVAY
                     LLQRVESYEGIVILATNLGRNIDEAFARRMQHTIEFPFPEAAQREQIWRGMLPQGAPI
                     GDDTDFAFLAKQFELSGGNIRNVVVAAAFQAAADGGVIGMRQMIAATARELDKIGRLP
                     SRSEFRDYYDWIREP"
     gene            5711..7123
                     /locus_tag="VNN25_27140"
     CDS             5711..7123
                     /locus_tag="VNN25_27140"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HXH95280.1"
                     /translation="MKINETPEKLEHKATSSARPSRAVSKRGLGRLVGNHAMQHLLRD
                     DPMEAEADRVAASAGKGVPSSLARAAGAARLHTDDAASDSAAAQHSAAYTVGRDVYFG
                     HGLYDPQSTEGATLLAHELTHVVQQNGGDEAAGIHAAPAGVARWPLAPSSTLYPRPEE
                     YVTFEEFAEAWPDRVTSMDTLHEIWNRQTRRGWTIRALQAVGIDPSTWLPRLGFRANI
                     DNINKVYEYYAGLYLENPRLRWAAMAKLAGGEVYRGLETELQPAIEFAEALDDHDPYT
                     IGLNDIYDAYAGSMILILLEMQQDIFVDLAWQHQAYREKGISALKMAWERGEISWDNY
                     QAWLDIDSADEDRIWEGNKSLLYREQSMILEGGEEQLGLGPPGPAGPKQGFHAHYGRI
                     RNIPDDDNIPKDMSEKALSPIPGGTRFSKVVPGGDITLLKDRWKWIETDMFPKFRALA
                     VADLRRYLAMPVRSLAKRKF"
     gene            7127..>8293
                     /locus_tag="VNN25_27145"
     CDS             7127..>8293
                     /locus_tag="VNN25_27145"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HXH95281.1"
                     /translation="MKASVGPQQSEHAEAAPSPAPQVVTPAQRLQRTAGNFAIQQFVQ
                     TKLRAGSPGDPLEREADRVADEVVGNDPAADMARIFGRDFSSVRMHTGESDAERARSL
                     GARAFTLGNDIVFGRGEYQPGTKEGQRLIAHELTHVVQQSRGSASAGEIHRKPDDPPP
                     DTTKIPAGETVEIPLERLKRWPANDLIVGVFGTNLVALPLKGEAWTLAPATEVGTVAE
                     PGPILTIPTVGKEGLVAVKVSGNVAFLIDAGGEQAVVSPTALAEIQSALGVTSIKGTV
                     ITHVHKDHIQSLVEIVKAAQIRPENLHFAEAFENILNPYLNALRNDASLQPLGYSATA
                     SFTKIATPTTGTFFSSTQVEGEMQFDYYGLTGPMRTLSAARTAGGLGKTADTGSL"
BASE COUNT         1561 a         2771 c         2669 g         1292 t
ORIGIN      
        1 ccgtgaagat cgcgaacgcg cgttcgcgat cttcacggag ctcgtgcaca tcggccgcga
       61 gtccatcgtg atcagcgcgc tcggcgaccg cggcgcggac gaagtgtggg agacggcgtt
      121 tccgatcgat gcctgcgagc catccgccga tctcctggca tcgattgcct cgttctggga
      181 ggagacgatc gatccgggca cgacgcacgg cgcgctccgc cttgttgcca cgctaatccg
      241 ccacggcgtg tccgagacac ccgcggccat gcgcgcgcac gtcgacgccg tgcgcagcct
      301 cgccgcctct gcgtctgccc ctgggtggca cccccagccc ggcgatgcat ccctccaaaa
      361 cgccgcgggg cagggggcgc cacactcaaa cgctctcctc atcgcgatcg gacgcagccg
      421 tcccgagctc gttacctccg tcgccagcac cgttcgcgcg gcggccatcg ccaccaacac
      481 gatgatcggg agcgcgttcg ccggctacgc tttgcttctg cctatcatcg cggagctcga
      541 gccgccgctc gagcgcgccg cgcgcgaact ggtgctcgcg aagtgcgcgg cggcggacga
      601 tgtgttcctc gatgcgattc tgcagcttgt gcagggcgac gatgtagaag acgagacaac
      661 gcgcagcgac gacgagctcg agaccatcgc gaacgaagtc ctcgcctcgt tcgggaaccg
      721 catccccggc ttcgctcgca gcagcgccgc gcatctgcgg gagaactttc ttgacggtac
      781 cggcgcgatc cgcttcgagc gggagcgtat cgaaatcctc ctgccgcacg taccgctggc
      841 gcttctgttg cgcatgttcg gcgtgcacgg caaaacgttc gccctgccat ggctgccaga
      901 gcgcacaatc acactgctca tgccggagga tgcatgagcg agcggaagag cgagcattcg
      961 gcgacacccg aagcgtcgcc ggaaccgcgg agatcatcga cttccacaca accgctgcag
     1021 atgctgctgc ggcgcgccgg caacaacgcc gtgtgcggaa tcgtgcaggc gaagcttcgc
     1081 gtcggcggcg cgagtgttcc gctcgaggag gaagccgaac gcgccgcgtc caccctcggc
     1141 ggcggatgtt gcgccgccaa gccggaaggc gaacaatgcg acgagtgcaa cgtgcgccgg
     1201 aaggcaatcg ctccggatca ggcatccgcc gacgcgccac cctccgtcga tcgggcactg
     1261 cacggctccg gcattccgct cgacgacgcc acccgcacct cgatggaatc gcacttcgat
     1321 tcggatttga gcgacgtgcg cgttcatacc ggcggcgacg cctcgcggtc cgccgacgag
     1381 attggcgccg ccgcatacac gtccggccgc gacatcgtct tctccaatga tggataccag
     1441 ccgcactccg gcgacggccg tcgcctcctc gcgcacgagc tgacccacgt actacagcag
     1501 cgcggccgga ccgatcgcac cgtacggcgc cagccggcga aaccgattcc caccaaagcg
     1561 gtcgacgatc cctgctcgat ctcggccacg tcgctcacca acgcggagct gatctccgtc
     1621 ttcgccaaca cacgcaacta cgtgaagaca cacaaacgcg gcgaggacca gctctggtac
     1681 gaccacggca atctcctccg ccggctggca tcggaggtcg atcgccgcag aaagctgcgc
     1741 cacgtctggc ttggcgatga atcgctcacc agcatgcccg gagtgttgta cgagctccgc
     1801 ggcggcatgg gcaatgccat tcagatctat ccggccgacg tgaaagcgga agcgggcgcg
     1861 ccgtacggcg acggcagggc gtccgtgatg acgaccggtc agttcaacgc ctacctggag
     1921 aagcagcaga tcaaacagat cgacgcccag gagtactttg caaagcagga cccgaagaac
     1981 atcgagccgc tgacgtacat gctgccggag cctcaggtcc cgaagtgggc gcagggacaa
     2041 cagatctggg gaccggccgc atcgcttctc ttttccaatc cgatgagcta cggcgcgaat
     2101 ccgttcaacc cgatcgggat ggcgggctcg gggtggggga tgaccgagct cacggccttt
     2161 ggcggatacc cgaatccgtt cggaacattc gccagcaagt ccgcatccca ggggatttac
     2221 aagaacaatg cgaacccggc gacgctatcc gtcagagagc agagcggcgg cgaggtgaac
     2281 tggcgcggcg acatacccga gatcaccacc gcgcggtcaa gctgggcgga tgtggtcatc
     2341 tactccgacc tcaacaaagt caaagccaat ttcgaggtct tcgactatcg caatcgtttc
     2401 agcggcgatc tcgtctctgt cacgcacacc cttcccgatc gcaagggcaa gacctcattc
     2461 aatcagtacg acacaaagtt tctgcgcgcg atcaatgccg atcaggcggc ggcagagcag
     2521 gcgaaattcg ggcgtgcgct gcagcagctc aatcagaagt acggcacgag cacgcaggtg
     2581 atcgatgcca acgagaagac gtatctggcc atcaacgagg accacgtcac cggctatcgc
     2641 acccagctcg cggccgacgt cgtcgccgat ccggggtcat acgcgccgct gctcgacgcc
     2701 ctgctgcgaa aagacccggt gacgtttcag aacgcggacg gcacgcgaac gacgatcagc
     2761 acttggaacg atctgcagaa tgcgcgtgct accaacgcca tccccgccgg cgacttcgat
     2821 atggcgatcg gcaggctctc cacggccacg agccagcgcg tcatcagcaa cggcatcacc
     2881 acgacccagc tccaggtgat gcagaaattc cgcgcccaga acttcggcgt cccgcaggag
     2941 acgtggagcc ggagcgttgc gccggagttg ctcgatgcga tgcgttacgg cgaaatcaac
     3001 ccgacaacgg gattggccga gcccaattac gccccgatgg tcaaatccgc cgcgaaaggg
     3061 ggcgccgtga tgggcgcggg attctctctt ttgacggacg ccgtccgctt cggaaggaat
     3121 cccggccaac atccggacat gtggcgcgag ggtctcgtcg atgtcggtct cggcggcctg
     3181 ggcggcgcgg ggagcgcctc cgtcgagagc tacgtcgccg cgcgaacatc agccgcgttg
     3241 atgggtgaaa ccggtggcgg ggcggcagcg atgcggatcc tcggccgcgc gggcggcggc
     3301 gcgctcggca gcctcgccgc gccggttgtg accatggcct cgatggccct cagctcgcaa
     3361 gactacacat cgctcgaata ctacggcgcg cttggccgca gcagcgccac cggggccgtc
     3421 ggcggaatcg gcggcgcgct ggcaacgggt acctacttcg cgatcgccgg aagcgagttc
     3481 cctctggtgg gcaacgccat cggtttcgtc gtcggcttcg gcggcgcgct gatcttcgac
     3541 gccatatggg gtgaagacat cgaccgcggc atccgcgagg cgggcggtga gctgggctgc
     3601 gtggacaagg ctccgccgaa atgaacgtgc atctcctcgt cgccgccgct ccgttcgccg
     3661 atgcccgcgc gcatctcgac gccgagctcg cgctgctcga tctccggctt cggcgccagg
     3721 taatgcggct gcgcgcggcc cggttgctga ccgaggatca gttccgcggc ctctacatcc
     3781 ccgacgatca ggcggatgcg attctgcagc gcgctgaaaa tgaggacgtt accgccgcag
     3841 cattgacgcg gcacatcgac gagctgcgcg gcgcgatcga ccggcgggcg gcactcaccg
     3901 tgcgcggcgg aaagacgctc ccgctcgtcg cactgcgtga acgtttcggt ctcactgcct
     3961 tcgagatcga gatcctcatc atcgccgtgg cgccggagat cgatcttcgc tatcacacgt
     4021 tgtacgcata cgcgcagaac gacatgacgc gccgctatcc gaccgttgat ttcgcgctgc
     4081 aactgctttt cgcgtcgcgc gaggatcgcc acgagcaccg cgccgcattc cgtccggacg
     4141 caccgctgat gcagcaccgc ctggtgcgcc tcggcgaaga cgcgcaggag aaagagcagt
     4201 cgctcctggc gcggccgctg cgcaccgacg agcgtgtgac cgaccagatc ctcggaggcg
     4261 atgcgctcga cccgatgctc gtcgcggccg ctcgttcggt gacaccccgg aagcggctcg
     4321 atgacctcgt attggccgcc gccgtgcgcg aacaggtact caacgtgatt cccctgttgg
     4381 cgcgcggcgg agcggcactc gtgctcgaag gcgcgcgcgg aaccgggcga aagagcatcg
     4441 cagaggcgat ctgcggagcg atgaacgcgg gaatgatcgt cgtcgatggc gctcgcgctg
     4501 ccacgctgcc cgatgccgca gcgctcctcc gccgcgaagc gcttctgtcc ggacgggcgc
     4561 tagtcatcga cgatttcgag aaactccccg ccggcagccg tttcgacggc gccattcgtt
     4621 tcctcatcac gacggagcct gtgtccgccg atggataccg tatcgacgtt ccgatgcctg
     4681 gggccgccat gcgcgcccgc ttgtggcgcg atgcgggggc agcgccggcg gacgatgcac
     4741 tagccgcaaa attcgtattg agcggagggc agattcgcgt cgcggttgag cacgcgcgcg
     4801 ccgccgccgc gcttcgaggc gcgccgggga tcgagctccg ggatctcatc gccggcgcgc
     4861 atgcgcagtc gagcgcgtcg ctccgcgcgc tggcacagaa ggtcggcctt cacttcggct
     4921 ggagcgacat cgtcctgcca gcgaagccgc tgcagcagct ccgggagatc tgcatctccg
     4981 tgcagtaccg ccgggtggtc ttcgacgatt ggggattcga ggggcgggtg gcgggcggaa
     5041 agggagtggc cgtgctgttc aacggcgcaa gcggcaccgg gaagacgatg gcggcttcga
     5101 tcatcgcgcg cgagctcggc ctgcagatgt atcgcgtcga tctctccacc gtggtcagca
     5161 agtacatcgg cgagacggag aagaacctca gccgcatctt cgccgaggcg caggcgagca
     5221 gtgccgtgct cttcttcgat gaagccgacg cactgttcgg caaacggtcg gaagtgaagg
     5281 atgcccacga tcgatacgcg aacatcgaag tcgcctacct gctgcagcgc gtggagagtt
     5341 acgaaggaat cgtcatcctg gccacgaacc tcgggcgcaa catcgacgaa gccttcgcac
     5401 gccgcatgca gcacaccatc gagttcccct tccccgaagc cgcgcagcgc gagcagatct
     5461 ggcggggcat gcttccgcaa ggcgcgccaa tcggagatga caccgacttc gcattcctgg
     5521 cgaaacagtt cgagctctcc ggcggcaaca tccgcaacgt cgtcgtcgcc gctgcgtttc
     5581 aggccgcggc cgacggtggg gtcatcggca tgcggcagat gatcgccgcc accgcgcgcg
     5641 agctggataa gatcggacgc ctgccatcgc gaagcgagtt tcgcgattac tacgactgga
     5701 ttcgagagcc atgaagatca acgagacgcc ggaaaagtta gaacataagg cgacctcttc
     5761 cgcacgtccg tcgcgcgccg tctcgaagcg agggctgggg cgtctcgtcg gcaatcacgc
     5821 aatgcagcat ctcttgcggg acgacccgat ggaagcggag gccgatcgtg tcgccgcgtc
     5881 cgccggaaag ggagtgccgt catcgctggc tcgtgcggca ggtgctgcgc ggttgcacac
     5941 tgatgacgcg gccagtgact cggcggcggc gcagcactcg gcggcgtaca cggtcggccg
     6001 cgacgtctac ttcggccacg gtctctacga tccgcaatcg accgaaggcg ccacgctcct
     6061 cgcgcacgag ctgacgcatg tggtgcaaca gaacggcggc gatgaagcgg ccggcattca
     6121 cgccgctccg gccggcgtcg cgcgttggcc gctggctccg tcgagcaccc tttatccgcg
     6181 acccgaggag tacgtgacgt tcgaggagtt cgcggaagcg tggccggacc gcgtcacaag
     6241 catggacacg ctgcacgaga tatggaatcg ccagaccaga cgaggctgga ccatccgtgc
     6301 gctgcaggcc gtagggatcg atccgagcac ctggctaccg aggttggggt ttcgcgccaa
     6361 catcgacaac atcaacaagg tctacgagta ctacgccggt ctctacctcg agaacccgcg
     6421 actgcgctgg gccgcgatgg ccaagctggc cggcggcgag gtctatcgcg gactggaaac
     6481 cgagttgcag cccgcgatcg agttcgcgga agcgcttgac gaccatgacc cctacaccat
     6541 cggattgaac gacatctacg acgcctatgc cggctcgatg atcctgatcc tgctggagat
     6601 gcagcaggat atcttcgtcg acctcgcctg gcagcatcag gcgtatcgcg agaaaggcat
     6661 cagcgcgctg aagatggcgt gggagcgcgg cgagatcagt tgggacaact accaggcctg
     6721 gctcgacatt gactcggccg acgaggaccg gatctgggag ggcaacaaaa gcctgctgta
     6781 ccgcgagcaa agcatgattc tcgagggagg tgaggaacag ctcggactcg gaccgcccgg
     6841 ccccgccggc cccaaacagg gcttccacgc gcactacggc cggatccgga acatccccga
     6901 cgatgacaac atcccgaaag acatgtccga aaaggcgctc tcgcccatcc cgggaggcac
     6961 gcgcttttcg aaagtcgttc ccggcggcga catcacgctc ttaaaagacc gctggaagtg
     7021 gatcgagacc gacatgttcc cgaagttcag agcgctggcc gtcgccgacc tgcgccgcta
     7081 cctggcgatg ccggttcgca gtcttgcgaa acggaagttc tgagccatga aggccagtgt
     7141 tggaccgcag caatcggaac acgcggaagc ggcgccgtcg ccagcaccgc aggtcgtcac
     7201 gccggcgcag cggctgcagc gcacggccgg caacttcgcc atccagcagt tcgtgcagac
     7261 gaagcttcgc gccggctcgc ccggcgatcc gctcgagcgc gaggccgatc gcgtggctga
     7321 cgaggtagtg ggcaacgatc cggcagcaga catggcacgc atcttcggcc gcgacttcag
     7381 cagcgtgcgc atgcacaccg gcgaatccga cgcggaacgc gcccgatcgc tcggcgcgcg
     7441 tgcgttcaca ctcggcaacg acatcgtctt cggccgcggc gagtatcaac ccggcacgaa
     7501 ggaagggcag cgtctcatcg cgcacgagct gacccacgtc gtgcaacaaa gccgcggcag
     7561 cgcgtcggcc ggagagattc accgcaagcc cgacgatcct ccgccggata cgacgaagat
     7621 accggccggc gaaacggtcg agatccctct cgagcggctc aagcgctggc cggcgaatga
     7681 cttgatcgtc ggggtcttcg gcacgaacct ggtcgcattg ccgctcaagg gcgaagcgtg
     7741 gacgctcgcg ccggcgaccg aggtcggcac cgtcgccgag ccgggcccga tcctgaccat
     7801 ccccaccgtc ggcaaggaag gtctcgtcgc ggtcaaggtc agcggcaacg tggcattcct
     7861 gatcgatgcc ggcggagagc aggcggtcgt ctctcccacg gcgctggccg agatccagtc
     7921 cgcactcggg gtcacgtcga tcaagggcac ggtgatcacg cacgtccata aagaccacat
     7981 ccagagtctc gtggagatcg tgaaggccgc gcagatccgt cccgagaacc tgcacttcgc
     8041 cgaagcgttc gagaacatcc tgaaccctta cctgaatgcc cttcgcaacg acgcctcgct
     8101 tcagccgctc ggatacagcg cgacggcgag cttcaccaag atcgccactc cgacgaccgg
     8161 cacgttcttc agctccacgc aggtcgaagg cgagatgcag ttcgactact acggactaac
     8221 ggggccgatg cgcacactgt cggccgcgcg aacggcgggc ggcctgggta agaccgcgga
     8281 taccggctcg ctg
//
LOCUS       DATLVW010000912         4703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_574717, whole genome shotgun sequence.
ACCESSION   DATLVW010000912 DATLVW010000000
VERSION     DATLVW010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574717"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VNN25_27150"
     CDS             <1..829
                     /locus_tag="VNN25_27150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95282.1"
                     /translation="LLHMPPRLGARVARFAVLGNDDIDIGQALIKQLPFGVPVDLAAA
                     YAERLAKAATGDLLQFELREWVDYVDAIEPAVWQARSSQLTAAWLKTRALIYGYFAAP
                     PGDLRITLRDAVTSLEESVRLSDPGARFTPARAKRLLALLDPGRHDARQLLLDAALAG
                     DAEAQRLLAVRAAESGDCKSAAAWFSSLLWKSNHSPEVPYDYAMTMMRGPCRTADRRR
                     AIELLSASAEGGVPSAMFELGVQMATGAPPAAGANVWIERAASFGHPGAIELVRESQ"
     gene            1370..2455
                     /locus_tag="VNN25_27155"
     CDS             1370..2455
                     /locus_tag="VNN25_27155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95283.1"
                     /translation="MPTKRDFIPLDSIDGLLLDEDESFEDYLFFHNVLDKFCWLIKLH
                     YADRETVRRKRYRLDTLFQWYLRTNRVSPVAARKSLAAIVSPDQLEHNLTKGWHNELV
                     RSDPLHAEYLQVGTNIGNWAGPGSGGLAAWNVVQSYYAIFEFLSAIVNAYNPDLRIDG
                     HKKLAREFNNHVQGKISGSLLFYPFTISSVTSDRAFPEHPAHTKFHYATYPRAVGTSI
                     DQLEKLVRDAFAFAGEGRRTSILDFLYELRLWANYTGVGRLLKLRDGGYQAFLMKNLG
                     TVVFFAGGIAELAYISLFGQARYAALVHKFAREYIDRSERFARQKYLIPIYVRLRAYK
                     HVGIFTGSLRFVFPDTEDPIQFIQRDT"
     gene            complement(2751..4598)
                     /locus_tag="VNN25_27160"
     CDS             complement(2751..4598)
                     /locus_tag="VNN25_27160"
                     /inference="COORDINATES: protein motif:HMM:NF036725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH95284.1"
                     /translation="MDETKADDLGLALSLTHLTYFAKDESPPPAPPIVDWPAFRDLVP
                     LSLTEREQLHALRDLVAGYVKEPENGRPLCVAVFGPPGSGKSFAVKQIAKLVESDLGT
                     RLTTRTINLTQVSDAGELASTIAAALLSTRSHEVPLLFFDEFDAPRAGATYGWLSWFL
                     APMHDGEFLHGARSIPVTRAIFVFAGGTAASMKEFSDRRDDRVFREAKGPDFVSRLRG
                     YLDVAGPNAEPRMLRRAYVLRAELQKRARRLGNQKTLTVDPDVLTALLHTGRYRHGAR
                     SLATLIELCNPSEVAIGTHVLDWDALPKDLILDLLVDRGPLDPRAIHGAIALSGFGTT
                     TAVDECWAAVASALWNEGATLAYAGQWQGHNRSLTERLAEELAARPRELRRTGDARPD
                     APPWFRSYLQGFDYARSRQEAQQMISADDWSALGIALEEPQTLADDERAWGEHEWKAR
                     VVERFRRRLAVSEASVARFVVGGNLEPTGGLPSGIIEEAVLTLGLGRPIYVAGGFGGA
                     AADLGVVLGLSALRTGAVPTSLAFQPSRTNEATLQEIAPRLRPPPLLDLPVLPQERVQ
                     FLQRHALGGPGWPPNGLTTEENRELFASDDVRCVARLVVTGLLRLFDKR"
BASE COUNT          829 a         1520 c         1463 g          891 t
ORIGIN      
        1 gcttctgcac atgccccccc ggcttggcgc acgcgtggcg cggttcgccg tactcggcaa
       61 cgacgacatc gacatcgggc aggcattgat caaacagttg cccttcggag ttccggtcga
      121 cctcgccgcg gcgtatgcgg agcgcttggc gaaagcggcc actggcgatc tcctacagtt
      181 cgagttgcga gagtgggtgg actacgtcga cgcgattgag cccgccgtct ggcaggcacg
      241 atcgtctcag ttgacggcgg catggctgaa gacgcgcgca ctgatctatg gctacttcgc
      301 tgcgcctccc ggcgacctgc ggattacgct acgcgacgcc gtcacgtcgc tcgaagaaag
      361 cgtgcggttg tccgatccgg gcgctcgctt cacgcctgcg cgtgcgaagc gcctcctcgc
      421 cctgctcgac cccggacgtc acgacgcgcg gcaactcctg cttgacgctg cattggccgg
      481 cgatgcggaa gcgcagcgcc ttctggctgt gcgcgcggct gagagcggtg actgcaagtc
      541 agccgctgct tggttctcct cgttgttgtg gaagagcaat cactcgccgg aagtgccgta
      601 tgactacgcg atgacgatga tgcgtggccc gtgccgcacg gcagatcgtc ggcgggcgat
      661 cgagcttctg tccgcatccg cggagggcgg cgtgccttcc gctatgttcg agctgggcgt
      721 gcaaatggcg accggcgcac cgcctgctgc cggcgcgaac gtctggatcg agcgggcggc
      781 gtcgttcgga catcccggag cgatcgagct cgtgcgggag tcgcagtaac tatagccgcc
      841 tcacagttca catgattggc ttagggatca gaccagggtc agatcttcgg ttattgtttt
      901 ccgccagagc gcgtttgttt cgaaccgcaa accactcgct gctcgcctcg tatctgctct
      961 ccggccgcgt caatccggaa tcgatcgcgg ccaccagatc gttcgcgcgc ggagtcaggc
     1021 accacgtccg ctcaggagac gtcggtgagc agctggtcac gccgctccgc gtcggccctc
     1081 ttccagatgg cggcgagatt gggggttagg agggtcaggc ctgtgccgca cggaatgagt
     1141 gcggttcggg aggcgtgatc caagagatcc agggaggcag ggctctcctc tgaggttgat
     1201 ggtaaaagct ccctcgagac tcgcgacatg tgctaaccga tggtggtgaa gttggggtcg
     1261 tttcaggaag atcaacttaa cgttcagccg ctaagttgat tttccagata cgaccccaac
     1321 tcatccgaga gccggatgaa ccccccgtgg taggttgtcg cggcaagcca tgccgaccaa
     1381 gcgagacttc atcccactcg attcaatcga tgggctcctc ctcgacgagg atgagtcatt
     1441 cgaggactac ttgttctttc acaatgtgct cgacaagttc tgctggctca tcaagttaca
     1501 ttacgccgac cgcgaaacag tacgacgcaa acgatatcgg cttgatacgt tgttccagtg
     1561 gtacctgcga acgaaccgag tctcgccagt cgccgcgagg aagagcctcg ccgctatcgt
     1621 gtctccggac cagttggaac acaacctcac gaagggctgg cacaacgaac tcgtcagaag
     1681 cgatccgcta cacgccgaat accttcaagt tgggacgaac atcgggaact gggcggggcc
     1741 cggttccggt ggtctcgccg cctggaacgt agttcaaagc tactatgcca ttttcgagtt
     1801 tctgagcgca atcgttaacg cgtataaccc tgaccttcgc attgatggac acaagaaact
     1861 cgcgcgcgag ttcaataacc acgtccaagg caaaatatcg ggctccttgc tcttttatcc
     1921 attcacgatc tcatccgtga caagtgatcg agcatttcca gaacatcctg cacacacaaa
     1981 gttccactat gcgacttatc cgcgcgctgt gggcacgtca atcgatcaac ttgagaagct
     2041 tgttcgcgac gcatttgctt tcgcaggtga aggccgcaga acatccattc ttgacttcct
     2101 ctacgaactg cgtctgtggg ccaactacac tggcgttggt cggttactga aacttcggga
     2161 tggtggctac caggcatttc tgatgaaaaa cctcggcacc gttgtcttct ttgccggtgg
     2221 catcgcagaa ctcgcataca tttcgctctt cggtcaagcc cgctatgccg cactcgttca
     2281 caagttcgca cgtgagtaca ttgaccgcag cgagcgattt gcgcggcaga agtatctaat
     2341 tcctatctac gtgcgcttac gtgcttacaa gcacgtcggc atcttcacgg ggtccctgag
     2401 gttcgtgttc ccggacacag aagatcccat tcagttcatc cagagggaca cctaactttg
     2461 gtgtgaagct gctgcggccc tgagtcggtc agtctgtcga agaagctcac ctccggccga
     2521 tgccgagctt gtgattcttg ataccgtcac ttcggtacga gcgacgaaag agccgtcttg
     2581 tcgaaggcag acgtcagttc gccgaccttg cattccagag caagcgccag acgcgggctt
     2641 tcaagatggg gtcaggtcta aagttgaaag cgacgaaagg gacgtcagca tcgagaatga
     2701 cacttctgct tcgtcacttt caactttaga cctgacccca ttttgaaagc ctaacgcttg
     2761 tcgaacagcc gcaggagacc ggtcactaca agccgcgcca cgcaccgcac gtcgtcgctc
     2821 gcgaaaagct cgcgattctc ctcggtcgtc aacccgttgg gcggccaccc gggcccgccg
     2881 agcgcgtgcc gctggaggaa ctgtacgcgc tcctgcggca gcacggggag gtcgaggagc
     2941 ggcggcggcc ggaggcgcgg tgcgatctcc tgcagcgtcg cttcgttcgt cctggagggc
     3001 tggaacgcga gcgacgtcgg gacggcgccc gtgcgcagcg ccgacaggcc gagcacgacg
     3061 ccgaggtccg ccgcagcgcc gccgaagccg ccggcgacgt agatcggccg gccgaggcct
     3121 agcgtgagca ccgcctcctc gatgatgccc gacggcagcc cgcccgtggg ctccaagtta
     3181 ccgcccacta cgaagcgcgc cacgctcgct tcgctcaccg ccagtcgacg gcggaatcgc
     3241 tcgacgaccc tcgccttcca ctcgtgctcg ccccatgcgc gctcgtcgtc cgcgagcgtc
     3301 tgcggctcct cgagcgcgat gccgagcgcg gaccagtcgt ccgcggagat catctgctgc
     3361 gcctcctggc gcgatcgggc gtagtcgaat ccctggaggt agctgcggaa ccatggcggc
     3421 gcgtcgggac gcgcgtcgcc cgtgcggcgc agctcgcgcg gacgcgccgc gagctcctcg
     3481 gccagccgct cggtgaggct gcgattgtgc ccctgccact gccccgcgta ggcgagcgtc
     3541 gcgccctcgt tccacagcgc cgatgccacc gccgcccaac actcgtcgac tgccgttgta
     3601 gttccgaagc cgctgagcgc gatcgcgccg tggatcgcac gcgggtccag cggcccacga
     3661 tcgacaagga ggtcaaggat gagatccttc ggcagtgcgt cccagtcgag gacgtgcgtg
     3721 ccgatcgcca cctcggatgg gttgcacagc tcgatgagcg tggcgagcga gcgcgcgccg
     3781 tgccggtatc gccccgtgtg cagcagtgcc gtcagcacat ccgggtcgac ggtcagggtc
     3841 ttctggttgc cgaggcggcg cgcgcgcttt tgcagctcgg cgcgcagcac gtaggcgcgc
     3901 cgcagcatgc gcggctccgc gttcggcccc gcgacgtcga ggtatccgcg cagccggctg
     3961 acgaagtcgg ggcccttggc ctcgcggaac acgcggtcgt cgcggcggtc ggagaactcc
     4021 ttcatgctcg ccgcggtccc gcccgcgaac acgaagatgg ctcgcgtgac gggaatgctg
     4081 cgcgcgccgt ggaggaactc gccgtcgtgc atcggcgcga ggaaccagct cagccagccg
     4141 tacgtcgcgc ccgcgcgcgg cgcgtcgaac tcgtcgaaga acagcagcgg cacctcgtga
     4201 ctgcgcgtgc tgagcagtgc ggcggcgatg gtgctcgcca gctcccctgc gtcgctcacc
     4261 tgcgtcaggt tgatcgtccg cgtcgttagc cgcgtgccca aatcgctctc gacgagcttc
     4321 gcgatctgct tcacagcgaa cgacttcccg ctcccgggcg ggccgaacac cgccacgcac
     4381 agcggccggc cattctccgg ctccttcacg taccccgcca cgaggtcgcg cagcgcgtgg
     4441 agctgctcgc gctcggtcag gctcagcggc acgagatccc ggaacgcagg ccagtccacg
     4501 atcggcggtg ccggtggcgg gctctcgtcc ttggcgaagt acgtgaggtg cgtcagcgac
     4561 agcgcgagtc cgaggtcgtc cgccttcgtc tcgtccataa tgatggccag cttgcaacac
     4621 atgtctgctg cacgcaagtg ccgagtggcg tacccgtccc cgtagcagca ctttgcttat
     4681 ctccgtgaga aggcgctagc gtg
//
LOCUS       DATLVW010000913        10023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_164489, whole genome shotgun sequence.
ACCESSION   DATLVW010000913 DATLVW010000000
VERSION     DATLVW010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164489"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..956)
                     /locus_tag="VNN25_27165"
     CDS             complement(165..956)
                     /locus_tag="VNN25_27165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95285.1"
                     /translation="MRRFTRFCRRYAGDRPFTTVLFFISVVGMGVLLPLAWSLYRARP
                     VRVTVVVGTLGKQPALKAISIRVQRISNGPDHMQVVASLDPAAIASGTTLYVTVPTTF
                     KIGPVDASSWEVHEAGGSDGRKDIHAKPASSNDHSGCTFDFTGEIVPKASSVDIQFGI
                     FGGHNGREVQVPTELTIADLDDIAFDQLIPEPEQRTSRFIWYTLGLWREKIADGVITL
                     RGTDRAAAVRSQFYLFVVATLIGVFASLVTNLAQAFVAAFELADD"
     gene            1085..2320
                     /locus_tag="VNN25_27170"
     CDS             1085..2320
                     /locus_tag="VNN25_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HXH95286.1"
                     /translation="MRKPTPTEAFVHQVCRQSFLSLWSYASPEGKTHGREMCDILVVC
                     DPDIIIFSVKEVRPTKHADDVVRMTRWVRAAVDESVDQVYGAERFIQSGKHTRVRRED
                     GSDGLPYPSKNVRRIHRVAVALGGEEHFPLPFGNFGKGFVHVFDERSFRILTSELDTI
                     TDFAEYLTTKEELFSGNTTVMFGHEEDILAVYLHGNRSFPSADHVLIEDGAWQQFIAK
                     PEVQAKKIADQDSYLWDRLIERIGRDVAARNMIYENTIEEDESVLRVMTRENRFNRRI
                     VAGQLRSFFEASAANKVRSRMIESLSGVGYVFLAAKRDEDRKMRSMELGLRCYVARLR
                     MDPCATIIGIGTEQKTGELGASWDVELLHTAEIDDAFRARAEEISKDLDYFQNPVQTH
                     LHEDEYPGICPEDHDTTPR"
     gene            2467..3789
                     /locus_tag="VNN25_27175"
     CDS             2467..3789
                     /locus_tag="VNN25_27175"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH95287.1"
                     /translation="MPEEAYLHQVLYMLIQDLAELWLEDSPGTRALHSLHEKQLERIA
                     RKIQDITGVKVTPKTLRNYIQRKIGEKSQYLDLIAAAWLVATKQLDEKAVSFADGTTD
                     NRFSQCVALFRRRNNEIMKCLSRPEGQTLEFKEEMPGPNSLANTLSAFANSSGGMIVI
                     GVNEHHLPLIDGLTGNEQALTRMNAALRRLDPEPMLEYGWFYVYDGDKKLFFIRIPAP
                     PSNHKLVKSFGQAYCRRGSSDAPVETGPRIRGPKCKVTAARRKLLTALRRVLTIMGSS
                     AAGEAHNVVATFSLIYIAEIFSTYLIDLMAEIHTRHTEDAGFVGDMNNVIEHRLEILR
                     RGDIEAFLVGMKSTPTIFRALAEGREILERVFSLARIWRRNGGLTDEEYVASFGGALH
                     TERALTVAEVRALAGGIHALSGKIDSATNSAYRLQELSMEDLPPSSWV"
     gene            complement(3852..4586)
                     /locus_tag="VNN25_27180"
     CDS             complement(3852..4586)
                     /locus_tag="VNN25_27180"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HXH95288.1"
                     /translation="MSNITDTATDTTTKRAVRLLSIFATFPPYYRHGRENEHLRWLLS
                     LSSLELDRVIRFFNAWRPLSRYQPQLLLLLASVFSDAQKQKTIIMGNAVEEGGWTDGH
                     DPHWALLDDLIVTLGGTPVVLKRSEELMVSFLNGLRRPMSEAYAVGILAGIENPALDI
                     SDYFREVVRLTGRSDLLESNLYLSIHVKVEPLHIIDTHEMALEYMARGPQERAEVLAA
                     FKEVMLFWSEFWNAAFNDLMDIRLAS"
     gene            complement(5083..7476)
                     /locus_tag="VNN25_27185"
     CDS             complement(5083..7476)
                     /locus_tag="VNN25_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95289.1"
                     /translation="MRLSRLAGGIVLIVASVSLTNAQQADNPHPSAYSTAIPQSLRGP
                     FISDPAVVMALECPTSACEGRLNFARLMQARSPEENVAGQNPFNLAVAAKILATVKSD
                     LTDVRTTAGATEGQLNPDFLTDSGSRVELVGAINRMDRQFIKDPTVGLTHPQLACGEV
                     SLIYRFSYSIRDGKQASRLPVTLNMVFPALPSRAVVRLLAPSKTRSVDCADIAQRWLD
                     EGMKPAGRTPQQQAADLLDPANGPLAFIDGRDLIRLELNMQAYRKGASSDDTDFGTAA
                     AYLIRVFKWEPHQKLFLPEFLRCQIDRTTVLCDPSDTKTACAEKQRRRKRLIRYLSRP
                     DVMASIDKGTLEVPYDYGVLATRALSISPGGLQRSANQPYWKALTPSQEVISDAEIAA
                     AMKKAHAAGVAFSFVKSADDFRTRLNETSCTGCHQTRAIAGFHFPGADRPGTPAVNAV
                     LLPGSPQFYGDQPRRKEIVTMMAKRLRRRLSEYELATSYSARPMKRFTDNLRDTQLIG
                     GWGAACIISTALPATQRQWTCREELGLTCTQLFASANDPAVGTCVPSGRPQVGDPLQR
                     GNVVSTAFGIDKYTRSAPVSPDQRIPASVLPSPPPAGNAYYGAHQEFYEGDATSTDYA
                     IRRDALTGGFPAGMLRLSECIGLPPEATCGLIASSGFNGCIGRLATDPNYTIGTCFSN
                     FTSYAGIRACDAGSPCRDDYICVMPIGYTPDTAEQLFKERAARMATSPYFQQVNGRAY
                     DPNDFGQKSPDAAWIARNDQRGLCIPPYFVFQFRSDGHPAPPELTPSSPAPGMSVNR"
     gene            7809..8063
                     /locus_tag="VNN25_27190"
     CDS             7809..8063
                     /locus_tag="VNN25_27190"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXH95290.1"
                     /translation="MVSTNNSKLLAGVLAAALLLPATTALGNDWSKPKSRTKGTAIGA
                     VAGAMVGGPPGAVVGAAVGNGVQVVRHQATARHHRHHRHY"
     gene            complement(8118..8849)
                     /locus_tag="VNN25_27195"
     CDS             complement(8118..8849)
                     /locus_tag="VNN25_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95291.1"
                     /translation="MKWIPFVALILLAATARGQDNYEIQVYGSETVAPGMTMVEVHSN
                     VTARGEQHAVRETLELTHGFNDWFETGFYVFTTAGSGQGWSYVGDHIRPRVRVPPEWH
                     WPVGVSLSTEIGYVRPRYAADTWSWEVRPIVDKTLGRWYVALNPALEKSLHGENAGRG
                     FEFAPAMNVKFDVTPKVAAGIEYYAGLGPISHFDPSAAQSHQIYPSIDLNVSPEWEFN
                     AGVGFGLTKATDRVIVKFIVGRRMH"
     gene            complement(8937..9785)
                     /locus_tag="VNN25_27200"
     CDS             complement(8937..9785)
                     /locus_tag="VNN25_27200"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXH95292.1"
                     /translation="MLELLGRHPYLRELVISTLIGLGVYVVCALVIVAAELRQRSDMS
                     VYHTRSALNDLAYAVFYKCSIYNILVTPLFSFLSPRMQFARIGLLSSLPPLVSVIIYW
                     IAIDFWNYWIHRLQHGVPVLWAFHSVHHTQTRLTFLSANRIHAIEQLYSGLLMIVPAF
                     LLGVPQQLWLPLLFMQLFSETLQHARLNWSFGSLHGLIVSPLFHKLHHSADEREYNGN
                     YGRVFSLWDAMFGTFVRSSTTQRQYGVTGMDVPDTLTAQFIHPFRYLMSRRARSLDPA
                     IEQAQP"
     gene            complement(9778..>10023)
                     /locus_tag="VNN25_27205"
     CDS             complement(9778..>10023)
                     /locus_tag="VNN25_27205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95293.1"
                     /translation="MLKEHLGTIPDNWFSNYAYAVLDGRGVARNVSRDYLARVALLAV
                     RAAFRWNGFPSVSLLRTLASSLLHAHATVPPRIAIHA"
BASE COUNT         2080 a         2914 c         3109 g         1920 t
ORIGIN      
        1 ttgaggtcta catacaactc ctccgggctc tcggcgcagc cgtcattttt cagcaacgtc
       61 gccttttcct ccgccgggcg atgaaattcc gaagacgctc gcctctgtag ggagtcgcag
      121 aatcgggcgt ttctccacct accccggtca agccgctcgc agggttagtc gtccgccagc
      181 tcgaacgcgg caacgaatgc ttgcgcgaga ttcgtgacga gcgacgcgaa gacaccgatc
      241 agtgtcgcta caacgaacaa gtaaaactgg ctgcgcaccg cagcggcgcg atctgtgccg
      301 cgcagtgtga tcacaccgtc cgcaatcttc tcgcgccaca atcccaacgt gtaccagatg
      361 aacctcgacg tgcgctgctc aggctcgggg atcaactgat cgaaggcgat gtcgtcgaga
      421 tcggcgatcg tcagctccgt cggcacctgt acttcacggc cgttgtggcc accaaaaata
      481 ccaaactgga tgtcaacgct ggaggctttc ggcacgattt cgcccgtgaa gtcgaaagtg
      541 caaccactgt ggtcgttcga cgacgcgggc ttcgcgtgga tgtccttacg accgtctgac
      601 cccccagcct cgtgcacctc ccacgagctc gcgtcgactg gtccgatctt gaaagtcgtt
      661 ggaacggtga cgtacagcgt cgtgccagac gcaatcgcgg cagggtcgag cgatgcaacc
      721 acctgcatgt gatcaggacc attcgaaatc cgttggacgc gaatcgagat cgccttcaag
      781 gccggctgct taccgagagt gccaacaaca actgtcacgc ggacgggacg tgcccggtag
      841 agggaccatg ccagcggcag gagaacgccc atgccaacaa cggaaatgaa gaagaggacc
      901 gtggtgaacg gacgatcacc tgcatatcgg cggcagaagc gagtgaaccg tctcatcgtg
      961 ggcgattgta gcggtgcgcg tccgcgaacc tactagcaag cgaggcgcaa tccaattcag
     1021 gacaaaggct gatttgtcag tcacgctgag gaacgcctgc gcttggaagt gtgttgctga
     1081 ctcggtgagg aaacccaccc cgacagaggc ctttgttcat caggtttgca gacagtcgtt
     1141 cctgtcactc tggagctacg caagccccga gggaaagacc cacggacgcg agatgtgcga
     1201 catcctcgtg gtctgcgatc cggacatcat catcttcagc gtgaaggaag tgcggccgac
     1261 aaagcacgct gacgacgttg tccggatgac gcggtgggtt cgcgctgcgg tggatgaatc
     1321 cgtcgatcag gtctatggcg ccgagcgatt catccagtcg ggcaagcaca cgcgcgttcg
     1381 cagggaggac ggctctgacg gtctcccgta cccgagcaag aatgtccgtc gaatccaccg
     1441 cgtggcggtc gcgctcggtg gggaagaaca cttcccgctt cctttcggca acttcggaaa
     1501 gggattcgtg catgtctttg atgaacggtc gttccggata ctgacgtcgg aactcgacac
     1561 gatcaccgac ttcgccgagt acctgacgac gaaagaggaa ctgttctcag gcaacacgac
     1621 cgtgatgttc ggccacgaag aagacatcct cgccgtgtac cttcatggaa atcggagctt
     1681 cccatcggcg gaccatgtcc taatcgagga cggagcctgg cagcagttca tagcgaagcc
     1741 ggaggttcag gcgaagaaga tcgccgacca ggatagctac ctgtgggatc ggctcatcga
     1801 gcgaatcggc cgagacgtcg ctgcacggaa tatgatttac gagaacacca tcgaagagga
     1861 cgaaagcgta ctgcgcgtca tgacgcgcga gaaccgcttc aaccgccgta tcgttgcggg
     1921 ccaactgcgc tcgttctttg aagcttccgc tgcgaacaag gtccgctcac gcatgatcga
     1981 gtcgctatcc ggcgtcggct acgtttttct ggctgcaaaa cgcgacgagg atcgaaagat
     2041 gagatcgatg gagctagggc ttcggtgcta tgtcgctcgt ctcaggatgg atccttgcgc
     2101 cacgatcatt ggcatcggaa cggagcagaa gacaggcgag cttggcgcgt cgtgggacgt
     2161 tgaactgttg cacactgcgg agatcgatga tgcgttccgc gccagggcgg aggagatatc
     2221 gaaggatctg gattactttc agaaccccgt tcagacccac cttcacgaag acgagtaccc
     2281 gggcatctgc cccgaagatc acgacacgac gccgcgctag tcgctcatgc cacgtgccgg
     2341 caacgatcgg ttgccagcgt ggatcggctc gatcgcgttc ccacctcacc gggatattgg
     2401 cggactcatg aataattccg tcgccagcac gagttctggt tctcgtagga acgaggagcc
     2461 ggaacgatgc cagaagaagc ctatctacat caggtgctgt acatgctgat ccaggatctc
     2521 gccgaactct ggctcgagga tagccccggt acccgtgcct tacattcact tcatgagaag
     2581 cagctcgaaa gaatagcaag aaaaattcag gatattaccg gggtgaaagt cacgccgaaa
     2641 acgcttcgga actacatcca gcgaaagatc ggagaaaaaa gtcagtacct tgacctaatc
     2701 gctgctgcgt ggctcgtcgc tacgaagcaa ctggacgaga aagccgtgag tttcgcagac
     2761 ggcacgacgg acaaccgctt cagccaatgc gttgcgctct tccggcgccg gaacaacgag
     2821 attatgaagt gcctttctcg gcccgaaggg caaacgttgg aattcaagga ggagatgccg
     2881 gggccgaaca gccttgcgaa tacgctctcg gcattcgcca attcttccgg tggaatgatc
     2941 gtcatcggcg tgaacgagca tcatttgcct ctcatcgacg gactgactgg taacgagcag
     3001 gctcttacgc gtatgaatgc tgcgctgcgg cgactcgatc ctgagccaat gttggagtac
     3061 gggtggtttt atgtgtacga cggcgataag aagctcttct tcatccgcat tcctgcgcct
     3121 ccgtcgaacc acaagctcgt aaaatctttt ggccaagcat attgccgtcg aggttccagt
     3181 gatgctcccg tcgaaaccgg acctcgaatc cgcggaccaa agtgcaaagt gactgccgcc
     3241 cggcgcaaac ttctcacggc tctgcgccgc gttctcacca ttatggggtc ttcggctgcc
     3301 ggagaagcgc acaacgtcgt agcgacgttt tcattgatct acattgccga gatcttttct
     3361 acgtatctga tcgacctgat ggcagagatc catacgcgcc acacggagga cgcggggttc
     3421 gtcggggaca tgaacaacgt catcgagcat cgacttgaaa tcctacggcg tggggacatc
     3481 gaagccttcc tggtcggcat gaagtcgacg ccgacaatct tccgggctct cgccgaaggc
     3541 cgggaaatcc tcgagcgcgt attcagtctt gctcggatct ggcgacgaaa tggcggactc
     3601 acggatgagg agtacgttgc gtccttcggt ggtgccttgc acacggagcg cgcactcacg
     3661 gtcgccgaag tccgcgcact tgcgggcggt attcacgcgc tttccgggaa aattgattca
     3721 gcgacgaata gcgcctacag actccaggaa ctctcgatgg aagacttgcc gcctagttcc
     3781 tgggtctgac gtcgatactc tcttgtggcg ttccgggaga acagcaagtc ccggaacgcc
     3841 acgccagaaa tctacgacgc gaggcggatg tccatcaggt cgttgaacgc ggcgttccag
     3901 aactccgacc agaacagcat cacttccttg aaggcggcga gaacctcggc gcgctcctgc
     3961 ggcccgcgcg ccatgtactc gagagccatc tcgtgagtgt cgatgatatg cagtggctcc
     4021 actttcacgt ggatagaaag atagaggttc gattcgagaa ggtccgaacg tccggtgagc
     4081 ctgaccactt ctcggaagta gtcgctgatg tcgagcgccg ggttttcaat gcccgccaag
     4141 attccgacgg cgtaggcttc cgacatcggt cggcgcaggc cgttgaggaa cgagaccatg
     4201 agttcttcgg aacgcttcag aaccaccggt gtaccgccca acgttacgat gaggtcgtcg
     4261 agaagtgccc agtgaggatc gtggccatcg gtccaacctc cttcttcgac cgcatttccc
     4321 atgatgatcg ttttctgttt ctgcgcgtcc gagaacacag atgccaggag aagaagaagc
     4381 tgcggttggt agcgagaaag aggacgccat gcattgaaga agcggatgac acgatcgagt
     4441 tccaaggagc tgaggctgag gagccaacga agatgctcat tctctcgtcc atggcggtag
     4501 tacggaggaa acgttgcgaa gatgctcaat aggcggactg cacgtttggt ggttgtgtcg
     4561 gtggctgtgt cggtgatgtt gctcatcgct tcctccttgt tttctctgtc gagagacatg
     4621 gtggagcgag agccgcagcg cccgcttgga aagttcgaac actatttgag ccaggctaga
     4681 aagatacggg aaaggtagag tctggatagt gggcacggat ctctaagatg cccacgctga
     4741 agtacttcct cacttcttgc cgacgcgcgt cttccgccgt acggtcatat ccccaccgca
     4801 tttcaccgtt ttgtatgctc aagactatga aggaccctcg cgaggttcga agaagcggtg
     4861 ccagaccttc gttctttctg cggtcgtgac ctgttgaacg atcaatactt gcgatctccg
     4921 caactggtga tcacatcggc ggccagctat tcgtagctcg agacacttcg aacgccggga
     4981 gtcgggagtc gagtgtcgag aggctatcta tctagcgagc agccggttct tctccgaccc
     5041 cccactcccc actcccgacc cgcccgaggc gcgacgattg cgtcagcgat tgacggacat
     5101 tccgggggcc ggcgagctgg gtgtgagctc cggtggcgcg gggtggccgt ccgagcggaa
     5161 ctggaagacg aagtatgggg ggatgcagag gccgcgctgg tcgtttctgg ctatccacgc
     5221 tgcgtcggga ctcttttgac cgaagtcatt cgggtcgtac gcgcgtccgt ttacctgctg
     5281 aaagtacggc gaggttgcca tccgcgccgc tcgctctttg aacagttgct ccgcggtgtc
     5341 gggggtgtag ccgatcggca tgacgcagat gtagtcgtcg cggcagggcg aaccggcgtc
     5401 gcaggcgcgt atcccggcgt atgaggtgaa gttgctgaag caggtgccga tcgtgtagtt
     5461 cggatctgtg gccaggcgtc cgatgcagcc attgaagccg cttgatgcga tcaatccaca
     5521 cgttgcctcg ggtggaaggc cgatgcattc ggagagacgc aacatacccg cggggaagcc
     5581 gccggtgagc gcgtcgcggc gaatcgcgta gtccgtcgat gtcgcgtcgc cctcgtagaa
     5641 ctcctggtgg gcgccgtagt aggcgttgcc ggccggcggc ggcgaaggaa gcacggaggc
     5701 cgggatgcgc tgatcggggc tcacgggcgc gctccgtgtg tacttgtcga tgccaaacgc
     5761 ggtcgaaacc acattgccgc gctggagcgg atcgccgacc tgagggcggc cggatgggac
     5821 gcacgtaccg acggccggat cgttcgccga ggcgaacaat tgagtgcaag tcagtccaag
     5881 ctcttcgcgg caggtccatt gccgttgcgt cgcaggcagc gcagtggaga tgatgcaggc
     5941 ggcaccccat ccaccgatga gttgcgtgtc gcgcaggttg tctgtgaagc gcttcatcgg
     6001 ccgggccgaa taactggtcg ccagctcgta ttcggacaac cggcgcctca gacgtttcgc
     6061 catcatcgtc acgatctcct tacgccgcgg ctggtcgccg tagaactgcg gggagcccgg
     6121 gagcaggacg gcattgacag ccggcgtccc gggccggtcc gcgcccggga aatggaaacc
     6181 ggcgatggcg cgcgtctgat ggcagcccgt gcaactcgtt tcattgaggc gtgtgcggaa
     6241 atcgtccgcc gacttcacga agctgaacgc cacgcccgcg gcgtgagcct tcttcatcgc
     6301 ggcagcgatc tctgcgtccg agatgacttc ttgcgacggc gtcaacgctt tccagtaagg
     6361 ctggttggcg gagcgctgca agccgcccgg cgagatcgac agggcgcggg tggcaagcac
     6421 gccgtagtca tacggcacct cgagcgtccc tttgtcaatc gaggccatga cgtcgggccg
     6481 actcaggtag cggatgagcc gtttgcgccg ccgctgtttc tccgcgcacg cggtcttcgt
     6541 gtccgaggga tcgcagagga ctgtggtgcg atcgatctgg cagcgtagga actcggggag
     6601 gaacagcttc tggtgtggct cccatttgaa gacacgtatc agatacgcgg ccgccgtccc
     6661 gaaatcggtg tcatcgctgg acgcaccttt gcggtaagcc tgcatgttca gttcgagccg
     6721 gatgagatcg cggccatcaa tgaacgccag gggtccgttt gcagggtcga gaagatcggc
     6781 ggcctgctgc tgcggcgtcc ttcccgccgg cttcattcct tcgtcgagcc agcgttgggc
     6841 gatgtccgcg cagtcgactg accgggtctt tgacggagca agcaaccgca ccaccgcccg
     6901 cgacggcaat gccggaaaaa ccatattcaa tgtgacggga agccgcgacg cctgcttgcc
     6961 gtcgcgaatg gagtaggaga accggtagat gaggctgacc tcgccgcagg cgagttgcgg
     7021 gtgggtgagg cccacggtcg gatctttgat gaactgccgg tccatgcggt tgattgcgcc
     7081 gaccagttcc acgcgcgacc ccgagtcggt gaggaagtcg ggattgagct gcccttccgt
     7141 cgcgcccgcg gtggtgcgca cgtcggtcaa gtccgatttc accgtggcca ggatcttcgc
     7201 cgcaacggcc aggttgaacg gattctgtcc cgcgacgttc tcctccggac tgcgcgcctg
     7261 catcagccgg gcgaagttca gccgcccctc acacgcagac gtcggacact cgagtgccat
     7321 gaccacggcc ggatccgaga tgaaagggcc gcgcagcgat tgggggatgg ccgtggagta
     7381 ggcagatgga tgtggattgt ccgcctgctg ggcattcgtc aacgacacgc tcgcgacaat
     7441 cagaacgatt ccgcctgcca accgcgaaag acgcatggag acccgagatt atatgcgggt
     7501 gtggggagtg gggagtgggg agtggggggt ggaagaagaa ctggtctcct aactcggcac
     7561 ccaccggaca gacgacacac gttggaacgt cttcgggagt cgggagtcgg gagtcgggag
     7621 tcggaggtcg agatgctagc agccgctgct tcttcgacac ccacacccca caccccacac
     7681 cccactcccc accccccccc acacacacac accgctgcat ccgctactaa accgcccgtt
     7741 ttcatcgact tacacggtgg ctaacctggt atctccttcg caactaacaa ccagcaagga
     7801 ggctcactat ggtttccacc aacaacagca agcttctggc aggtgtgctc gccgcggccc
     7861 tccttctacc ggccacgacc gcacttggga atgactggtc caagccgaag agcaggacga
     7921 aaggtacggc gatcggtgcc gttgcaggcg ccatggtcgg tggccctccg ggagcggttg
     7981 tcggtgccgc ggttggtaat ggcgtgcagg tagtaaggca ccaggcaaca gcccgccacc
     8041 accgccacca tcgacattac taagcatcga gtacgcgagg ggcgagattc tcgcccctcg
     8101 ccattcagac cccgcagtca gtgcattcgc ctaccaacga tgaacttcac gatcacccgg
     8161 tccgttgcct tggtgagacc gaacccgacg ccggcgttga attcccactc cggcgaaaca
     8221 ttcagatcga tcgatggata gatctgatgg gactgcgccg cggacggatc gaagtgcgag
     8281 ataggcccga ggccggcgta gtactcgatg ccggcggcga ccttcggcgt gacatcgaac
     8341 ttcacgttca tggcgggggc gaactcgaat cctcgtccgg cgttctcgcc gtgcagcgat
     8401 ttctcgagcg cgggattaag ggccacgtac cagcgaccga gcgtcttgtc gacgatcgga
     8461 cgtacctccc acgaccacgt atcggcggcg tacctcgggc ggacataccc gatctccgtc
     8521 gagaggctaa caccgaccgg ccaatgccac tcgggcggca cccgcactcg cggccggata
     8581 tggtcgccga cgtagctcca accttgaccc gatccggccg tcgtgaagac atagaacccc
     8641 gtctcgaacc aatcgttgaa gccatgcgtc agctccagcg tctcccgtac cgcgtgttgc
     8701 tcccctcggg cggtcacatt gctatgcacc tccaccatcg tcatgcccgg cgccaccgtc
     8761 tcggacccat agacctgaat ctcgtagtta tcctgaccgc gcgccgtggc ggcgagcaga
     8821 atgagcgcga cgaacggaat ccatttcatg gcgatcattt cagcacaccg cgcgatgtat
     8881 ttgccgccgc caggttggcg acgcgaaaga cgttgccgga agttgatttg gccgcctcag
     8941 ggctgcgctt gctcgatcgc ggggtcaagg gaacgcgctc gccgcgacat gaggtagcgg
     9001 aatgggtgaa tgaactgggc ggtcagcgtg tcggggacgt ccattccagt cacgccgtat
     9061 tggcgttgcg tcgttgacga acggacgaac gttccgaaca ttgcgtccca gagcgaaaag
     9121 acgcggccgt agtttccgtt gtattcgcgc tcgtcggccg agtgatgaag tttgtggaag
     9181 aggggactca cgatcagccc gtgcagggag ccgaatgacc agttgaggcg ggcgtgctgg
     9241 agcgtctcgc tgaatagctg catgaaaagc agcggcagcc acaattgttg cggtacgccg
     9301 aggagaaagg cagggacgat catcaacaag ccgctataca gctgctcgat ggcgtggatg
     9361 cggttagcgc tgagaaatgt caaacgtgtc tgggtgtgat gaacgctgtg aaacgcccag
     9421 agcaccggca cgccgtgctg cagccggtgg atccaatagt tccaaaagtc gattgcgatc
     9481 caatagatga tgacggagac gagcggcggt aatgaggaca acagtccgat gcgtgcaaac
     9541 tgcatgcgtg gggagagaaa cgaaaagagc ggtgtgacca ggatgttgta gatgctgcac
     9601 ttgtagaaga ccgcgtacgc gaggtcgttg agtgcgctcc gcgtgtgata cacgctcata
     9661 tcgctccgct gccgcaattc ggcggctacg atcaccaggg cgcacaccac atagacgccc
     9721 agtccgatca gcgtcgatat gacgagctcg cggagatacg ggtgacggcc gagcagctca
     9781 agcatgaatc gcgatccttg gaggaacggt ggcgtgtgcg tggagcagcg atgaagccag
     9841 tgtccgcaga agagagacgg acgggaagcc gttccatcga aacgcggcgc ggactgccag
     9901 gagcgctacg cgcgcgaggt aatcgcggct gacattgcgc gctacgccgc ggccgtccag
     9961 taccgcgtag gcgtaattcg aaaaccagtt gtcggggatc gttccgaggt gctctttgag
    10021 cat
//
LOCUS       DATLVW010000914         3817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_616519, whole genome shotgun sequence.
ACCESSION   DATLVW010000914 DATLVW010000000
VERSION     DATLVW010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616519"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1257
                     /locus_tag="VNN25_27210"
     CDS             253..1257
                     /locus_tag="VNN25_27210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXH95294.1"
                     /translation="MLSKQQRRRSSIALRRAAEGESVRTIVIALLANLVIATAKLMAG
                     LASHSTGMLAEAAHSAADSVNEIFLAIGLHREKDPPDEIHPLGHGRERFLWAFMAAIA
                     SFLIGGCLSIVLAIRELETRHPVGATMAAWIVLAISFAADGASWLQSMRQARRQAGEY
                     NLTVWRYLVRASDPVVRAIVVEDSAALAGLTIAAGGLLASRVAGTNVPDSIASLLIGL
                     LLAVTAFGVARPLADFLIGRSLPPEQLKDVHALVSQNPAIEEVLTLRAVYSGPEEVVV
                     IAKVHPSPDMNIDELSRAMDGLDHKIRSTFPLVADVFIDVTNARGDGAITRAKSRHER
                     "
     gene            1247..1771
                     /locus_tag="VNN25_27215"
     CDS             1247..1771
                     /locus_tag="VNN25_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH95295.1"
                     /translation="MSDEKKVIIIAGPNGAGKTTFAREFLPEEAGLPLFVNADLIAAG
                     LSPFDPDAAAFRAGRLMLSEIDAHAAAGRSFAFETTLAGHTYLRRIDQWRGNGYLVEL
                     IFLSLPSVEDAIQRVATRVRQGGHAVAEVVIRRRFDAGMRNFQHTYRERVDFWIWYDN
                     SGTRRRLVERGRNR"
     gene            1768..1977
                     /locus_tag="VNN25_27220"
     CDS             1768..1977
                     /locus_tag="VNN25_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95296.1"
                     /translation="MRDTETFEDNADMAGVGPALLRAARRAREIARQTGTPLVIVRDG
                     VLMEVDPDDPSLDDEAMAADTIRDR"
     gene            complement(2072..2713)
                     /locus_tag="VNN25_27225"
     CDS             complement(2072..2713)
                     /locus_tag="VNN25_27225"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH95297.1"
                     /translation="MARSRNRALALTGLGAGCAGAFAWLSAMVLQGRANGIDRRIHEW
                     LEERANPALDSAMDLVGALSAPAFLVTATLFIAFALRRRGRAVSLPIVFSPLVAMTTG
                     SLMTALMPPRYPPDAARCGYEPCFPSGHTTGATSELLVSSYVLMREQILPPSLATGVL
                     SIPLLAGCARVYRGRHWYTDVAAGWLMGITIATLCAATYESGKKRSGPAVPRT"
     gene            complement(2890..>3817)
                     /locus_tag="VNN25_27230"
     CDS             complement(2890..>3817)
                     /locus_tag="VNN25_27230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH95298.1"
                     /translation="YDEAIAKYLAILADDPSNGLASYELAYSYQAKGDYAKCIAVLKP
                     LSVAESVLLPKILGSLGNCLDGAGDAQGAVSAYRKSLALAPDDPGTLYNLGVTLVGQS
                     KLDEAREVLKKELIARPGHASGHYALARVFDAQNFRAAAILEYLRFLALEPSTPRARI
                     AAANLLALLNLGVEDKGVGKDGKKGINVAIDPNSRTEEGDFHGWEMGISIMSAGRFTT
                     EDKDKKATEFDSVRGQLGGTLTMLIELKDAPADYSGKQNIPFFVAIDQHKLLDTFAGA
                     ALQSLGLKGSDAWMKENAKGLQDLAAFIAGPH"
BASE COUNT          723 a         1159 c         1213 g          722 t
ORIGIN      
        1 tgttgttgtc attgcgacgc ggggggctcg tcacgggggt gtcgcacgtg cggcacgggg
       61 ggcgtagcgt tgcgacacga ctgctggcgc gtgcgcaacg aggcgacagc attgctgccc
      121 gccgagcgcg ggttgtgcca acccaaatga tttgagcgac ttgtgagaat gttgtctgtt
      181 ccatgttgcg tgttgcatgc gtgccatcct cggttcatgc aacgctcaac caaatagaaa
      241 gcaagaccac tcatgctgag caaacagcag cggcggcgtt cctccatcgc tctgcggcgc
      301 gccgcggagg gagagagcgt ccgcacgatc gtgatcgcgc tgctggcgaa cctcgtcatc
      361 gcgacggcga agttgatggc cggcctggct tcacactcga cgggcatgct ggccgaggcg
      421 gcacattctg cggcggattc ggtcaacgag atctttctag ccatcggcct gcaccgcgaa
      481 aaggatcccc cagacgaaat ccatcccctc ggccacggac gcgagcgttt tctctgggcc
      541 ttcatggctg cgatcgcatc gtttctgatc ggcggctgtc tctccatcgt cctggcaatc
      601 cgggagttgg agacgcgtca cccggtcggc gcgacgatgg cggcgtggat cgtgctggcg
      661 atctccttcg cggccgatgg cgcgtcatgg ctgcagagca tgcggcaagc gcgccgtcag
      721 gccggcgagt acaacctcac cgtgtggcgc tacctcgtcc gcgccagcga tccggtcgtc
      781 cgcgccatcg tggtcgaaga cagcgcggcg ctggccggtc tgacgattgc tgcgggcggc
      841 cttctcgcga gcagagtcgc tggaacgaac gtgcccgatt ccatcgcctc gctgctgatc
      901 ggactccttc tggccgtgac cgcattcggc gtggcccggc cactggccga tttcttgatc
      961 gggcgatccc tgccgccgga gcagctgaag gacgttcacg cactcgtcag tcagaatccg
     1021 gcaatcgagg aagtgttgac gcttcgcgcg gtctattcag gtccggagga ggtcgtcgtc
     1081 atcgcgaagg tgcacccatc gccggatatg aacatcgacg agctttcgcg cgccatggac
     1141 ggcctcgatc acaagatccg atcgaccttt ccactcgtcg ccgacgtctt catcgatgtg
     1201 acgaacgcgc gtggtgatgg tgccatcaca agagcgaaat cacgtcatga gcgatgagaa
     1261 gaaagtgatc atcatcgcag ggccgaacgg cgcgggaaag acgacgttcg cccgcgagtt
     1321 tctgcccgag gaggcaggat tgccgctgtt tgtgaatgcg gatctgatcg ctgctggact
     1381 gtcgccattc gatcccgacg ccgccgcctt tcgagccggg cgtttgatgc tctccgagat
     1441 cgacgcgcac gccgccgcgg ggcgaagttt cgcgttcgag acgacgctgg cgggacatac
     1501 gtatctgcgg cgcatcgatc agtggcgtgg caatggttac ctcgtggagc tgatctttct
     1561 gtcgctgcct tccgtcgaag acgcgatcca gcgcgtcgca acacgggtga ggcagggcgg
     1621 acatgcagtc gcagaagtcg tgattcgccg cagatttgac gccggcatgc gaaactttca
     1681 gcatacatac agagaacgag tcgacttctg gatctggtac gacaatagcg gtacgagacg
     1741 acgccttgtg gaacgagggc gcaaccgatg agagacaccg aaacctttga agacaacgcc
     1801 gatatggcag gggttggacc agcgctgctt cgcgccgccc gccgcgcccg cgagatcgcg
     1861 cgccagaccg gcacgcctct ggtgatcgtc cgcgatggcg tgctgatgga ggtcgatccg
     1921 gacgacccca gcctcgatga cgaagcgatg gcggccgata cgattcgtga tcgttgaagt
     1981 gcccaatcat cgaggccagt gtttcaccgt tgggcgggac gcccaacggc cgcgtgcgag
     2041 acgcccgcac tccttgcgct gatcgctgtt ttcaagttct tggaacggcc ggtccgctgc
     2101 gctttttgcc cgattcgtac gttgcggcgc agagagtggc gattgtgatt cccatcagcc
     2161 atccggcagc gacgtcggta taccagtgac gacctcgata gacgcgcgca cagcctgcca
     2221 gtagagggat cgacaggacg ccggtcgcca acgacggtgg aagaatctgc tcccgcatca
     2281 gaacatagga gctcaccagc agttcggatg tcgcccccgt cgtgtgaccg cttggaaagc
     2341 acggctcata gccgcagcgc gcagcatccg gtggataacg agggggcatg agcgcggtca
     2401 tcaacgagcc tgtggtcatg gccacgagcg gagaaaagac gatcggcagc gagacagcgc
     2461 gtccacgccg gcgaagcgcg aatgcgatga agagcgtggc cgttaccaga aaagcgggag
     2521 cggagagagc gccgacgaga tccatcgcgg agtcgagagc cggattcgca cgctcttcca
     2581 gccactcgtg aatccgccga tcgattccgt ttgcccgccc ctgcagaacc atcgcactga
     2641 gccacgcgaa cgccccggca cagcctgcgc cgagaccggt cagcgcgagg gctcgatttc
     2701 gggagcgcgc catacggctc ctgatagcaa cgttgatacc gtttcggtgc tgatcaccaa
     2761 ggatcagcgt tgaggcgacc gtgaatcaac cgggcgcgtg atggaggtcg acccagacga
     2821 ccccagcctc gatgacgaag cgatggcggc cgatacgatt cacgatcgtt gaaggtcgac
     2881 gtctcccctt cagtgcggac cggcaatgaa cgccgccagg tcctgcaacc ccttcgcgtt
     2941 ctctttcatc caagcgtcgc tgcctttgag gccgagggac tggagagctg cgccggcgaa
     3001 ggtgtcgagg agtttgtgct ggtcgatggc cacgaagaac gggatgtttt gctttccgct
     3061 gtagtcggcc ggcgcatcct tcaattcgat gagcatcgtc agcgtaccgc cgagctgtcc
     3121 acgcacgctg tcgaactctg ttgccttttt gtccttgtcc tccgtcgtaa agcggccggc
     3181 ggacataata gagatcccca tctcccagcc gtgaaagtct ccttcctcgg tgcgcgagtt
     3241 tggatcgatc gcgacgttga tgcccttttt cccgtccttt cccacgccct tgtcctcgac
     3301 gcccaagttc agaagtgcga ggagatttgc ggcggctatc ctggcacgcg gcgttgacgg
     3361 ctcgagggcc aggaagcgga gatattcgag gatcgcggca gcgcggaaat tctgcgcgtc
     3421 gaacacacgg gctaaagcgt agtggccgct ggcgtgaccg gggcgtgcga tgagctcctt
     3481 cttaagaacc tcgcgggctt cgtccagctt cgactggcca acaagagtga cgccgaggtt
     3541 gtaaagcgta cccggatcat cgggagcgag agcgagggac tttcgatacg ccgataccgc
     3601 gccctgcgcg tcacccgcgc cgtcgaggca attaccgagg cttcccagaa tcttcgggag
     3661 caacaccgac tcggctacgg acagaggctt caagaccgcg atgcacttcg cgtaatcacc
     3721 ctttgcttga tacgaatacg ccagctcgta ggaggccaag ccattcgaag gatcatcggc
     3781 aagtatcgcc aggtacttgg cgatggcctc gtcgtat
//
LOCUS       DATLVW010000915         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_328524, whole genome shotgun sequence.
ACCESSION   DATLVW010000915 DATLVW010000000
VERSION     DATLVW010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328524"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..2089
                     /locus_tag="VNN25_27235"
     CDS             92..2089
                     /locus_tag="VNN25_27235"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HXH95299.1"
                     /translation="MTALPIALSDPRLPKTDNRNIELLFLAMFSAIPLYVTQTISMFP
                     LLVFHAAMLAITARVFFNKGPELIPSAVMRALAIAYVPFYVVDAALISRSAIAASTHL
                     VLFITVYQSIESVRSRNTTQRLLTASLIALASIATATHIAILPFIILFGVLLLRQLMQ
                     VSHDETEAMTGARLPAPPTGRAAALYVCVAMALGVLFFPFLPRIRNPLLPNMSGAMEK
                     ATTGISDSINLNEPRSIASDATVVSRVWMGPDAIPFFTPLRLKGTVYERFTNNTWRQG
                     RYTFVPIDSVEGNARVARPEGFTRRASVQQRFPVGNRLFLPVGTYQVSGAGQVVEGPT
                     RDIYSVWQPHRDNLSYDVAIAHSTTPLRPQRVRVANYPVTPAVRAMARQLAGAETAPL
                     RRAAAIETYLSTHFTYVPDPAAIGHAMTVDQFLLQERRGHCEYFAAGMVALMTALDVP
                     ARIVGGFYGGTLNPLTGYFSIRREDAHAWVEVWDGQTWQTFDPTPAALRPGNAHEGLV
                     RMYAAAVSDWVNYFWDRHILTFGLGDQVALASDMIDRLRLAIAAMSAAARGAAHPTFN
                     VNYVFLLTALLLAGFAAAVLSRRRRSVFDLLADHLRKLGIDAGPAMTMEEALAEVRRT
                     QPAAAAALRPLIELYEAERFSANVERGRVRRIRRGLAELKT"
BASE COUNT          466 a          886 c          702 g          501 t
ORIGIN      
        1 gagcgagcga cggatcgtcc gagcaacctg gcaacgcagt aaccaagcta cccagcaact
       61 caaccgagca acccaacctc ggagcccgat catgaccgcg cttccgatcg cactctccga
      121 cccccgactc ccgaaaaccg acaaccgcaa catcgagctc ctgttccttg cgatgttctc
      181 ggcgattcct ctctacgtca cgcagacgat ctcgatgttt ccgctgctcg ttttccacgc
      241 ggcgatgctg gccattacgg cgcgcgtgtt tttcaacaag ggaccggaac tcattccctc
      301 ggcggtgatg cgcgcgctcg ccatcgccta cgtcccgttc tacgtcgtcg acgcggcctt
      361 gatctcgcgc agcgcgatcg cggcgtcgac gcacctggtt ctcttcatca ccgtttatca
      421 gtcgatcgag tcggtgcgca gccgcaacac gacgcaaagg ctattgaccg cgtcgctcat
      481 cgccctggct agtatcgcca cggccacgca catcgcaatc ctccctttca tcatcctctt
      541 cggcgttctg ctccttcgtc agctcatgca ggtcagtcac gatgagacgg aggcgatgac
      601 cggcgcccga ttgcctgcac cgccgaccgg gcgcgccgcc gcgctctacg tctgcgtcgc
      661 gatggccctc ggcgtcctct tcttcccctt ccttccgcgc atccgcaatc cgctgttgcc
      721 gaacatgtcg ggggcgatgg agaaggcgac gaccggcatc agcgactcca tcaacctgaa
      781 cgagccgcgc tcgatcgcca gtgacgccac cgtggtcagc cgcgtctgga tggggccgga
      841 tgcgattccc tttttcactc cgttacgctt gaaggggacg gtctacgagc gcttcacgaa
      901 caatacgtgg cggcagggcc gctacacctt cgttccgatc gatagcgtcg aaggcaatgc
      961 gcgtgtggcg cgaccggaag ggttcacacg gcgagccagc gtgcagcagc gttttccggt
     1021 cgggaaccgc ctcttccttc ccgttggaac gtaccaggtt tccggcgccg gccaggtggt
     1081 cgaagggccg acgcgcgaca tctactcggt ctggcagccg caccgggaca acctgtcgta
     1141 cgacgtcgcc atcgcgcaca gcactacgcc gctacgtccc cagcgcgttc gggtcgcgaa
     1201 ctatccggtg acgcctgccg ttcgggcgat ggcccgccag ctcgccggcg ctgaaaccgc
     1261 tccgctccgc cgtgccgcgg cgatcgagac ctatctctcc acgcacttta cctatgttcc
     1321 cgatcccgca gcgatcggac acgccatgac cgtcgaccag ttccttcttc aggagcgccg
     1381 cggtcactgc gagtacttcg ccgccgggat ggtggccttg atgaccgcgc tcgacgtccc
     1441 ggcgcgcatc gtcggtggtt tctacggcgg tacgctcaat ccgctcaccg gctacttcag
     1501 catccgccgc gaggatgccc atgcctgggt cgaggtgtgg gacggccaga cgtggcagac
     1561 gttcgatccg actcctgcgg cactgcggcc gggcaacgcc catgaaggcc tcgtccgcat
     1621 gtacgccgcc gctgtcagcg attgggtcaa ctacttctgg gaccgccaca tcctcacctt
     1681 cggcctcggc gaccaggtcg cgctggccag cgacatgatc gaccgcttgc gactggccat
     1741 tgcggcgatg agtgccgccg cacgcggcgc ggcgcatccc accttcaacg tgaattacgt
     1801 ctttctcctg acagcactgc tgctcgccgg tttcgccgca gccgtgttat cgcgccggcg
     1861 tcgatccgtc ttcgatcttc tggcagacca tctgcgcaag ctcggcatcg acgccggccc
     1921 ggccatgacg atggaagaag cactcgccga ggtgcgccgc acgcaaccgg ccgccgctgc
     1981 ggcgctgcgg ccgctcatcg agctgtacga agcggaaagg ttctcggcca acgtggagcg
     2041 gggaagggtg cgcaggatcc ggagggggtt ggcggagttg aaaacgtaag aggttcccta
     2101 tcggttcggg tcgggccacg cagtcaatct ggcggaaaaa gcgtttcacg cggagacgcg
     2161 gagcacacgg tggagacgta tccagtgacg attccatcaa aaatgtcgtc aatttcctcg
     2221 aaatcaacgc cgacgttgag gaggaatgtc ctcaactttc aggacaatcg tcctcaactt
     2281 caggacgatc gtcctcaact ttcaggacaa tcgtcctcaa cttcaggacg accgtcctca
     2341 actttcagga caatcgtcct caacttgcat gacgaccgtc attaactttc atgaccattg
     2401 tcatcaactt tcatgacgac cgtcatcaac tttcatgacc atcgtcatca actttcatga
     2461 cgattgtcct caacattcgg caaattttac ccaaactaaa gagggatcgt cctcaactcc
     2521 cactcccact cccactccca ctccccaccc cccac
//
LOCUS       DATLVW010000916         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_38137, whole genome shotgun sequence.
ACCESSION   DATLVW010000916 DATLVW010000000
VERSION     DATLVW010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38137"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2086
                     /locus_tag="VNN25_27240"
     CDS             <1..>2086
                     /locus_tag="VNN25_27240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95300.1"
                     /translation="ARKAGDKAALADFALESVRRLHDDRWFAHSHLFAHRHPEASAPA
                     HREMVAAINRRIPRLSIEGFRGIGKTTYTEETALLKAVFREFHNLVIIGPSFARACDR
                     VTAIRNEIDTNPYFAEDGLFGKLKGETWADGKIVLGADIMIQALGREQSITGLKFLQW
                     RPDAFIIDDIEDPEEERTDAEREKTWRWLKQTFQPCLDDALRTWGRFLGTRRGKNSLP
                     ERLENDRMPVVKFPIESIGENGERVATWPSKFPIEKIDALREDYRGDLNLYEQEFMCR
                     AASDAARIFKREMFKYEERVRIWEAVYGMVDPARTSHGNAAATGYAIWSWVKNRLVVW
                     ACDALFLAPDEIVALCFDLCERFDLVQLGIERDGLEQFLLQPLRHEQVKRGVMLPLRP
                     IAAISGTQGRGQTRFIEGLQPYFQAREVIFAQPFPALEAQLLSFPHGIRDAANALAYA
                     PTLRPAQPIYDGFDPVTHIAEGIDIAAGQPLALAANATGGLTCAILVQAFEGTLRILA
                     DWVYEGSPAERVADIAQAAAQVVDSSRFVAVPVPRPWDNMLKVPLPDRMLARPNRPAW
                     IVPQLHSDRHMNVGLMQAVRAIPAEVRVGGTEVDGTLYIRDALSRTVRGMPALEVSPA
                     AKWTLRAFAGGYTRGMIRGRLQDSAEEGIYRVLMEGLEAFCGSIRFATVDSEEDQAQN
                     YRIDERTGRSYAS"
BASE COUNT          386 a          701 c          647 g          352 t
ORIGIN      
        1 cgcgcgcaag gccggcgaca aggccgcgct cgccgatttc gccctcgaat ccgtccgccg
       61 cctccatgac gaccgctggt ttgctcactc ccacctcttc gcccaccgcc accccgaagc
      121 ctcggccccc gcacatcggg agatggtcgc cgcgatcaac cgccggatcc cgcgcctctc
      181 gatcgagggc tttcgcggca tcggcaagac cacctacacc gaggaaaccg cgctcctgaa
      241 ggcggtcttc cgcgagttcc ataacctcgt cattatcggc ccctcgttcg cccgcgcctg
      301 cgatcgcgtg accgccattc gcaacgagat cgacactaac ccctacttcg ccgaggatgg
      361 cctcttcggc aagctaaagg gtgagacctg ggcggacggc aagatcgtcc tcggcgccga
      421 catcatgatc caggcgctcg gccgcgagca gtcgatcacc gggctcaaat tcctccagtg
      481 gcgccccgat gccttcatca tcgacgacat cgaagatcct gaggaagagc gcaccgacgc
      541 cgagcgcgag aagacctggc gctggctgaa gcagacgttc cagccctgcc tcgacgacgc
      601 gctccgaacc tggggccggt ttctggggac aagacggggg aaaaactcgc ttccggaacg
      661 cctcgagaac gaccggatgc cggtcgtcaa gttcccgatc gagagcatcg gagagaatgg
      721 cgagcgggtc gcaacctggc cctcgaaatt cccgatcgag aagatcgacg cgctcaggga
      781 ggattaccgc ggcgacctca acctctacga acaggagttc atgtgccgcg ccgcgagcga
      841 tgccgcacgc atcttcaagc gcgaaatgtt caagtatgag gagcgggtca gaatctggga
      901 ggcagtctat ggtatggtcg accccgctcg caccagccac ggtaacgccg ccgcaaccgg
      961 atatgcaatt tggtcgtggg tcaagaaccg gctggtggtg tgggcctgcg acgcattatt
     1021 cttggctccc gacgagatcg ttgccctctg cttcgaccta tgcgaacgct ttgatcttgt
     1081 gcagctcgga atcgagcgtg acgggcttga acagttcctg ctccaaccgc tccggcacga
     1141 gcaggtaaaa cgcggggtga tgctgccgct ccgcccgatc gccgcgatat caggcacgca
     1201 ggggcggggg cagacccgct ttatcgaggg gctgcaaccc tattttcagg cccgggaggt
     1261 gatctttgcc caacctttcc ctgcccttga ggctcaactg ctcagcttcc cgcacggcat
     1321 tcgcgacgcg gcaaatgcgc ttgcatatgc gccgacgctg cgcccagccc agccgatcta
     1381 cgacggcttc gatcccgtca cccatatcgc tgagggcatc gacatcgccg ccggccaacc
     1441 cctcgccctt gccgccaacg ccaccggagg actgacctgt gcgatcctgg ttcaagcgtt
     1501 cgagggcacg cttcgcatcc tcgccgattg ggtctacgaa gggagcccgg cggaacgggt
     1561 tgccgacatc gcgcaggccg ccgcgcaggt ggtagacagt agccgcttcg tcgcggtgcc
     1621 ggtcccgcgc ccgtgggaca acatgctcaa ggttccgctg ccggatcgga tgctcgctcg
     1681 accgaatcgt ccggcttgga tcgtcccgca gctccactcc gaccggcaca tgaatgtcgg
     1741 gctgatgcag gcggtgcgcg cgatccccgc ggaggttagg gtcggcggca ccgaagttga
     1801 cggcacactc tatataagag acgcgctctc gcgcacggtg cgcgggatgc cggcgctcga
     1861 agtcagccct gccgcgaagt ggacgctgcg cgcattcgcc ggcggctaca ccagagggat
     1921 gatccggggc cggttgcagg acagcgccga ggaaggcatc taccgcgtct tgatggaggg
     1981 gctggaagcc ttctgcggtt ccattcgctt cgccacggtg gacagcgagg aggatcaggc
     2041 gcaaaactac cggattgacg agcgcaccgg gcgcagttac gcgagc
//
LOCUS       DATLVW010000917         2268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_450200, whole genome shotgun sequence.
ACCESSION   DATLVW010000917 DATLVW010000000
VERSION     DATLVW010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450200"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1303
                     /locus_tag="VNN25_27245"
     CDS             227..1303
                     /locus_tag="VNN25_27245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXH95301.1"
                     /translation="MKAALPTGTVTFLFSDIEGSTNLSRQHGAAYGDLRADHRRLLGD
                     TFGAHDGSVVDAEGDALFAVFVRATDAVAAAVAAQRALAGMGAVRVRIGIHTTEPHIH
                     SDGYVGVGVSRAARICAAAHGGQIVLSHAAAGIIEDQEVRGLRLRDLGEHRLKDISHP
                     QRLFQVEAEGLLSEFPPLATAAAGSIATLLVTDLVGWGRIMREFGDDAAAGVAAAYYR
                     VVNDCVDAHDGRVVERGGDHTLSLFSAAEEAVIAAAQMREALRKGDWTAAGTEMPAIC
                     AAIHTGRLARSAEGLLGSPAFRVLRLCATAEPGQILVSHATHSLLEGQILGQLSLRDL
                     GERALPGEKPARVYELVDESPHVD"
     gene            1700..2101
                     /locus_tag="VNN25_27250"
     CDS             1700..2101
                     /locus_tag="VNN25_27250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95302.1"
                     /translation="MRRMLFATVACLAVVCASQTAAAAQASTRLEVPPRIATALRQAG
                     FPVQKRCGFVGERGAGVPACWLSIEQSGYSVHVILHPSLRDAKVVYRRLSNPWARGTQ
                     VAMFGKLIVSGFRVPPREWQTIVTTVKRSTR"
BASE COUNT          389 a          707 c          751 g          421 t
ORIGIN      
        1 ttgagatgcc tccacggcaa tgccccaagt cagagccgat tcacggagat cgcccggcgc
       61 agctaggcaa tcgctgtcac tgatctggcc accagcgcac atagggcgca aagggcgagg
      121 cgtcacgctc gtgatctaac ggcaggtaag cctgggttgg gccagcgtgc gggaatgtca
      181 ccagagaagc taggcctgct gttcgtcgct gcttacgatc cagggcgtga aggctgctct
      241 tcccactggg acggtcactt ttctcttcag cgacatcgag ggatcgacca acctctcccg
      301 gcagcacggt gcggcctatg gcgatctgcg agcagatcac cgccgccttc tcggcgatac
      361 gtttggtgct cacgacggat cagtcgtcga cgcagaaggt gatgcgttgt ttgcagtctt
      421 tgtgcgcgcg actgatgccg tggcagcggc cgtcgctgcc caacgcgcgc tcgcaggaat
      481 gggagccgta cgcgttcgga tcggaattca cacgaccgag ccgcacatcc atagcgatgg
      541 ctatgttggc gtcggcgtga gtcgggccgc acgtatctgc gcggcggccc acggcggaca
      601 gatcgtcctg tcgcacgcag ccgccggaat aatcgaggac caggaggtcc gaggtcttcg
      661 gttacgtgat ctcggcgagc accgcttgaa ggacatctcg cacccgcagc ggttgttcca
      721 ggttgaagcc gaaggattgc tcagcgagtt cccgcctctg gcgacggcgg ccgcgggctc
      781 catcgcgacg ttgctcgtaa ccgatctcgt cggctggggc cgcatcatgc gggagttcgg
      841 tgacgatgct gcagctggcg tggccgccgc ctattacagg gtcgtcaatg actgtgttga
      901 cgcgcatgac ggccgcgtgg tcgagcgtgg cggggaccac acgctgtcgc tcttttcggc
      961 agcagaggag gcggtcatcg cggcagctca gatgcgcgaa gcgttgcgga aaggagactg
     1021 gaccgcagcc ggaaccgaga tgccagcgat ctgcgcagcg atccatacag gccgattggc
     1081 acgctccgcc gaaggcctgc tcggttcgcc agcgtttcgc gtgcttcggc tctgtgcgac
     1141 cgccgagccg gggcagatct tggtgtcgca cgcgacacac tcgctgctcg aaggtcagat
     1201 ccttggccag ctctccctcc gcgatcttgg agagcgcgcg ctaccaggcg agaagccggc
     1261 gcgcgtctat gagctggttg acgagtcgcc tcacgtggac tagccgggag ggcctctcgc
     1321 ctgggccagt gtcccggaat gtcactagag cgcaggcgct actgtcggtg tcgtgagccg
     1381 agagcaagcg cagactgctt ccgcaaggct gcgcccgctc gtctcgcggc ggggtcgctg
     1441 atgctgcggt tttcaacggt ggctccgggt cggtagaggg ctagaccctg gtagagcacg
     1501 gctcgactct cgctcgccct ctcaccgtga ccactacagc cggtggcaag aactacgagc
     1561 aaaaggcgag agcagcacat ttcaccgtca tgctcctgac aatgcgccgc tgagagccca
     1621 aatagtcacg acgaggagtg tcctcccgac tgagccagcg tgcgggaatg tcaccagaag
     1681 aggtcgtatc atccgactcg tgaggcgaat gttgttcgcg acggtcgcgt gtctggcggt
     1741 cgtctgcgct tcgcagaccg cggctgcagc acaggctagt acccgcctcg aagtcccgcc
     1801 gcggattgcg acggcgctgc ggcaagcggg ctttcccgtt cagaaacgct gcggctttgt
     1861 cggtgaacgc ggcgccggcg ttccggcttg ctggctcagc atcgagcaat cgggctactc
     1921 ggtgcacgtc atcttgcatc ccagcctccg cgacgccaag gttgtgtacc ggcgactgtc
     1981 gaacccgtgg gctaggggga cgcaagttgc gatgttcggg aagctcatcg tctcgggctt
     2041 tcgggtgccc ccacgtgagt ggcagaccat cgtcacaacc gtcaaacgct caacccgtta
     2101 ggcgcgcggc tcgcgtgggc cagtgtccgg gaatgtcacc cgaccaacgg agcgccgccg
     2161 cttgcctggt ctgacggagt tctcttgcgt cgtaggctgt cgagagcgtg ccgccccaga
     2221 ttcgatacgc ccacagcgac ggtgtgaata tcgcctacca ggtcgccg
//
LOCUS       DATLVW010000918         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_450280, whole genome shotgun sequence.
ACCESSION   DATLVW010000918 DATLVW010000000
VERSION     DATLVW010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450280"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VNN25_27255"
     CDS             complement(<1..407)
                     /locus_tag="VNN25_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXH95303.1"
                     /translation="MKNCKAAFLLPVLAVLTASCLEAADRPKLIVQITVDQLRGDLPF
                     RYRDRFTEGGFRYFLDHGTWYSDAYHPHSLTETVVGHTTLATGAYPSRHGMVANSWFD
                     RSTNTFIDNIKSPQYPLLSVNGETQTGSGGSPV"
     gene            459..1421
                     /locus_tag="VNN25_27260"
     CDS             459..1421
                     /locus_tag="VNN25_27260"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXH95304.1"
                     /translation="MLDIGQPESPQSLPLIVSAGAQSAGSPEGLLERYASERESMEAH
                     LLQHGALLFRGFGIDTQEILSRVVRPLGGEALGYIDGNSPRRKLGSGVYTSTEYPPEF
                     FISLHNELSYSSRWPSRVFFCCAIAPQEGGQTPLADSRRILQRLPDDVRDAFVTRQVK
                     YIRNLHGGSGFGPSWQATFETDSRDEVSEFCRTEGTEARWNDDGSLTITQVRPATRIH
                     PKTGEEVWFNQADQFHPSTHPAVIYESLLALYGREELMPQTATFGDGSPIEVASLDEV
                     RRTVGEEMRLFSWQRGDLLMVDNMLVCHGRRPFSGPRKILVSMT"
     gene            complement(1955..2299)
                     /locus_tag="VNN25_27265"
     CDS             complement(1955..2299)
                     /locus_tag="VNN25_27265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HXH95305.1"
                     /translation="MVISRGDVWWADLPEPTGSGPGLRRPVVIIQGDAFNRSRIATVV
                     CVPLTTNTSWADLPGNVLLPAAITGLPRDSVANASQVIAFDKTLLLQRVGKLSSSKLG
                     LVLSGLDIVLGR"
     gene            complement(2293..2529)
                     /locus_tag="VNN25_27270"
     CDS             complement(2293..2529)
                     /locus_tag="VNN25_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXH95306.1"
                     /translation="MKTAVSIPDEVFEKVERLARREKRSRSEVFSAALREYVARHNPD
                     EITDAINRVCDQVGDQRDEFVAAAARRVLEKTEW"
     gene            complement(2658..>2911)
                     /locus_tag="VNN25_27275"
     CDS             complement(2658..>2911)
                     /locus_tag="VNN25_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95307.1"
                     /translation="EVCNDPDLAQPLAPNDLIFFEVYRDADAYSEHLRGAVNEFIVAN
                     RDYFHAPWQGHPRPETTYLDPKLLFVRPGIEGNGNGNGG"
BASE COUNT          563 a          907 c          891 g          550 t
ORIGIN      
        1 ctgaccggcg acccgccgga tcccgtctgc gtttcgccgt tgaccgaaag gagcggatat
       61 tgcggcgact tgatgttgtc gatgaacgtg ttcgtcgatc gatcgaacca cgaattcgcc
      121 accatcccat ggcgcgacgg atacgcgccc gtcgccagcg tcgtatgacc gacgaccgtc
      181 tccgtgagcg agtgcggatg ataggcatcg ctgtaccatg tgccgtgatc gaggaagtag
      241 cggaagccgc cttcggtgaa gcggtcccgg tagcgaaacg gaagatcgcc gcgaagctga
      301 tcgacggtga tctgcacgat gagcttggga cgatcggcag cttccaggca ggaagcggtc
      361 agcacggcga ggaccgggag caggaacgcg gccttgcagt tcttcattag aatcactccg
      421 attgtattcg tggatggatg taggtgatta tcgctcaaat gctcgatatt ggacagcctg
      481 aatcgcccca gtcactgccg ctgatcgtga gcgccggcgc gcaatcggcc ggttcgcccg
      541 aggggctgct ggaacggtac gcgtcggagc gcgagtcgat ggaggcgcac ctgctgcagc
      601 acggcgcgct cctctttcgc ggattcggta tcgacacgca ggagatcctt tcgcgcgtcg
      661 tccgccccct gggtggcgag gctctcgggt acatcgacgg caattcgccc cgccggaagc
      721 tcggctcggg cgtctacacg tcgaccgaat atccgcccga gttcttcatc tcgctgcaca
      781 acgagctgtc gtactccagc cggtggccct cgcgagtctt cttctgttgc gcgattgcgc
      841 cgcaggaagg aggccagaca ccgctggcgg acagccgccg catcctgcag cgcctgccgg
      901 acgacgtccg cgacgctttc gtgacgcgcc aggtgaagta catccgcaat cttcacggcg
      961 gcagcggctt cggcccgtca tggcaggcca cgttcgagac ggacagcaga gacgaggtgt
     1021 cggagttctg ccgcacggag ggcaccgagg cgcgctggaa cgacgacggc agcctgacga
     1081 tcacgcaggt ccggccggcc acgcgcatcc atccgaagac gggggaggaa gtgtggttca
     1141 accaggccga ccagttccat ccgtcgacgc atccggccgt gatctacgag tcactgctcg
     1201 cgctgtacgg acgtgaggag ctgatgccgc agaccgcgac gttcggagac ggaagcccga
     1261 tcgaggtggc cagcctcgac gaggtgcgca ggacggtggg cgaggagatg cgcctctttt
     1321 catggcagcg cggcgacctg ctcatggtcg acaacatgct ggtctgccac ggccggcgcc
     1381 cattctccgg cccgcggaag atcctcgtat cgatgacgta gccttcccga gatcagggtc
     1441 agcccggcct gtgcgaataa ctgacgaaac atcgtgacgg ccagtccagg tgcacactca
     1501 caagtaaccg tttttctatg gtttgcatgg agggaacgag ccaaggcttg accgcctgcc
     1561 ttcgtcgttt tcaaacggag cgccgctgat tcgaaacggt gcgcctccgt ttcgaaacga
     1621 cgcgcctctg attccaaaca gcgcgcctcc gtttcgaaag ggggcgtctc tgatatcaaa
     1681 cgacgcgtct ccgtttcgaa agggagcgtc tttgattcca aacggcgcgt ctccgtttcg
     1741 aaagggagcg cctctgattc caaacgacgc gtcttcgttt cgaaacgtag cgtttctgat
     1801 tacaaacggc gcgtctccaa tatcgatggg aatgacatcg tacgccgttc cccttcgcgc
     1861 ccttcgcggc ttcgcgtgac accgatgctc acgcgaagcc gctcacgcga agccgcgaag
     1921 aagacttctg ggaacagtgc ccctgatgct accgctatct ccccagaaca atatcgagac
     1981 cagacaggac caacccgagc ttggaactgg acagctttcc tacccgttga agcaatagtg
     2041 tcttatcgaa cgcgatgacc tgcgaggcat tcgcgaccga atctctgggc aggccagtga
     2101 tcgccgcggg caagagcaca ttgcccggca aatccgccca gctagtattc gtggtcagtg
     2161 gaacgcaaac gaccgtggcg atgcggcttc gattgaaggc gtcgccttga atgatcacta
     2221 ctggacgccg aagaccggga ccggagccgg tgggttcggg aagatccgcc caccagacgt
     2281 cgcctcgcga gatcaccact ccgttttctc cagcacgcga cgcgctgccg ctgcaacgaa
     2341 ttcgtcgcgc tgatctccca cctgatcgca aacgcggttg atagcgtcgg tgatctcgtc
     2401 cgggttgtga cgggcaacat actcgcgcag cgcggcactg aatacctcgc tcctcgacct
     2461 cttttcgcgc cgcgccaggc gctccacttt ctcgaatacc tcgtcaggaa tcgacaccgc
     2521 cgttttcata ccgccagtat aaccgctttg gattccaacg aatcaaaccg agagtctccg
     2581 gtccccttcg cgccttcgcg gcttcgcgtg acactgcgct cacgcgaaga cgcgaagaag
     2641 actccgggga acggtactca gcctccgttg ccgttaccgt tcccttcgat gcccggacgg
     2701 acgaaaagca gcttcggatc gaggtacgtc gtctctggac gtggatgtcc ctgccacggc
     2761 gcgtggaagt aatcgcggtt ggcgacgatg aactcgttga cggcgccgcg gagatgctcg
     2821 ctgtacgcgt cggcgtcgcg gtagacctcg aaaaagatca ggtcattcgg cgccagcggc
     2881 tgggcgaggt cggggtcgtt gcagacctca t
//
LOCUS       DATLVW010000919         4647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_450385, whole genome shotgun sequence.
ACCESSION   DATLVW010000919 DATLVW010000000
VERSION     DATLVW010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450385"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VNN25_27280"
     CDS             complement(<1..774)
                     /locus_tag="VNN25_27280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oligopeptidase"
                     /protein_id="HXH95308.1"
                     /translation="MTETIEVARPTIPIYNASALSAAADKALSDARRALAAIESLPLE
                     ALTPESVLEAWDRISITLEDAYGPVSILNSVHPDAAVRDAGDRALIEESVFMTELFQN
                     EQLFERVRRVEPRTAAQRQLKKDLLEAFEDSGVALPRERRDRFKAISERLTELAQEFS
                     RNIRENKTLLHFTPDECAGLPQSYLDRVARDDQGNIVVGFDYPDYVPFMANAVSEEAR
                     KRYYIANTNRGTARNLEILDEIVALRKETADLYDAPSYAH"
     gene            904..1764
                     /locus_tag="VNN25_27285"
     CDS             904..1764
                     /locus_tag="VNN25_27285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH95309.1"
                     /translation="MRPIIAALAGLIALPLLAQNSFMDQGRAAMTRNDAEAASALFEK
                     AVAQNPKSADAHYWLGSAYGSMAQKASFFSQASLASKTKEDFEKAVELDPNHLEARMG
                     LVEYYAFAPGFMGGSFEKASAQAAEIRKRDPLMGHRASAFIYSQQKKTAEAKNEYLAE
                     VKEFPKSPRAHLDLGILHISEKDYKAAGIEFDSALTLDPLYMPAVFRIGQLAVLSNGN
                     LAHGEEMLRKYLAYTPKKDEPPLARAHYWLGQLFEKQGKKAEAKANFAASLKLNPNQK
                     DVAEALKRVS"
     gene            complement(1972..2181)
                     /locus_tag="VNN25_27290"
     CDS             complement(1972..2181)
                     /locus_tag="VNN25_27290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95310.1"
                     /translation="MPLLTEALRFAENPATSVDQLRRACDLLALDSSGTPSEVRGRLL
                     AHLETLDAASPIVCLNPNVKSAGET"
     gene            complement(2343..4442)
                     /locus_tag="VNN25_27295"
     CDS             complement(2343..4442)
                     /locus_tag="VNN25_27295"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXH95311.1"
                     /translation="MEASVRDPEARAVRTVQLVTIVIAAFLAVQTVAAAPSTFCIEQR
                     CQAVNEIPGTVAPSDTVRDFVWISSDLKQIATGTIQAGANVLTLPASSQHHKLAPRLS
                     DGSTRATLDFELRCQDLTWKWSVTRVPVPGVDLRHPGSGCTLLVKSNGYRSVERQLRD
                     VEVGNVFLQKIPILSGTVIDAKTAIPLRDARVYLPNGNSLAVTDRKGNFRAPVADLWP
                     QFIRVAMAGYARRTVLLPKAVADTELSVRLSKGGSLTIRLAPPLGQEDVTWEVRRPLA
                     GSRGDELAHSGRLPKGEKEAVVDALEPGKYRVIVAGSNPLQRIGQLVTLTEGGEAEAI
                     VQISAAVLEATVRYDDKPLAGAILHIEPEDSSWTSSVTTDSGGSVTEEVWQQGTYTAI
                     VTQLPLIPFWRETEHIDGDKNRWSISVPNRRLLGRVTDDSSGQPIRDARVEVTMRNDD
                     RSTMVTLRSGEDGRFELGAFPTGSYALAVSKSGYQSLQTSWSTVDETATDQSPQVRLK
                     AAGNRPVVVTNSFGAPLSAAVYVSTRNGTHPVGATDEDGRILLPVSPEDYGMVFVVPR
                     SGSIGVAQFESLGKGADDIHIRVPEGNASLEIDCETTSGAALTGVAVLLRIDGVLLPI
                     PVWEGMINYQGLPMFSDGQGRLLLTHLPAGRYEIWPLDRQQLNAVTSGSPPPAQVIIA
                     VTAGYQSAKMVFASAKP"
BASE COUNT          942 a         1418 c         1404 g          883 t
ORIGIN      
        1 gtgtgcgtaa ctgggggcat cgtagaggtc ggcagtctct ttccgcagcg ccacgatctc
       61 gtcgaggatc tcgagattgc gcgccgtccc tcgattcgtg ttggcgatgt agtagcgctt
      121 tctcgcctcc tcgctgacgg cgttggccat gaaggggacg tagtcgggat agtcgaatcc
      181 gaccacgatg tttccctggt catcgcgcgc aacacggtcg agatacgact gcgggagacc
      241 ggcgcattca tcgggcgtga agtggaggag cgtcttattc tcgcggatgt tccggctgaa
      301 ctcctgagcc agttccgtga gccgctcgga gatggccttg aagcgatcgc gccgctcgcg
      361 cggcagcgcc acgccgctgt cctcgaacgc ttcgagaagg tccttcttca attgccgttg
      421 cgcggccgta cgcggctcga cgcggcgcac gcgctcgaac agttgctcgt tctggaacag
      481 ctcggtcatg aacaccgact cttcgatcag ggcccggtcc ccggcgtcac ggacggcggc
      541 atcggggtga acactgttga gaatggagac cgggccgtag gcgtcttcca gcgtgatcga
      601 gatccggtcc cacgcttcga gaaccgattc gggtgtgagc gcttcgaggg gaaggctttc
      661 gatcgccgca agcgcgcgcc gggcatcgga tagagccttg tccgccgcag cggaaagcgc
      721 cgaggcgttg tagatcggga tagtgggacg ggctacttcg atcgtttcag tcatcgttca
      781 agttatcgac gggaggcgca ttctaggagg ctccgcgaga aaacgcacgc aacgcctttc
      841 agcggacaac ctcacgcccg cgctgagccg aaacatgttc tacgcaaatc gcgtactaga
      901 tgcatgcgtc caatcattgc tgccctcgcc ggattgatcg cccttcctct gcttgcccag
      961 aattcgttca tggatcaggg ccgcgcggcc atgacccgca acgatgcaga ggcggcgtcc
     1021 gcgctcttcg agaaagcggt cgcacagaat ccgaaatccg ccgacgcgca ctactggctc
     1081 ggcagcgcct acggatcgat ggcccagaag gcaagtttct tcagtcaggc ctcgctcgca
     1141 tcgaaaacga aagaggattt cgagaaggcg gtcgagctcg acccaaacca tctcgaggcc
     1201 cgcatgggac tcgtcgagta ctacgcattc gcccccggct tcatgggcgg aagcttcgag
     1261 aaggcatccg cacaggccgc cgagatcagg aaacgcgatc cgctcatggg acaccgcgcc
     1321 tcggcgttca tctattcgca gcagaagaag accgccgagg cgaagaacga gtatctcgcc
     1381 gaggtgaagg agttcccgaa atcgcctcgt gcccacctcg acctcgggat cctgcacatc
     1441 tccgaaaagg actacaaggc cgccggcatc gaattcgact cggcgctgac gctcgatccg
     1501 ctctacatgc ccgccgtttt ccgcatcgga cagctcgccg ttctcagtaa cggaaatctc
     1561 gcgcacggcg aagaaatgct gcgcaaatac ctcgcgtaca cgccaaagaa agacgagccc
     1621 ccgctcgccc gcgcccacta ctggctcggc caactcttcg agaagcaggg aaagaaagcg
     1681 gaggcaaagg ccaactttgc ggcgtccctc aagctcaatc caaaccagaa ggatgtggcc
     1741 gaggcactga agcgggtttc gtagcgctca tcgaacgcgg gaaatcgatc gtagcgccgg
     1801 cggcctcgcc ggctgtaccg ccagcggccc gctggcgact ccagccctcg ctgcaaatta
     1861 acgatcgcga gacccgcagc ggataatcgc ccgcgcccga gctgccggac gtcaatggct
     1921 acgtcgactc gatcgcgccc cggcctccat acgcgtcatc tgcggatcgc tctatgtttc
     1981 accagcagat ttgacgttgg gattcagaca gacgatcggc gacgcagcat ccaacgtctc
     2041 caaatgcgcc agcagtcgcc cgcgaacctc ggatggcgta cccgaggagt cgagagccag
     2101 gaggtcgcag gcgcgacgga gttggtcaac gctcgtggcg ggattctccg caaaccgcaa
     2161 cgcctcggtg agaagtggca tagagagtca tccgcagatg acgcagatac acacagatga
     2221 agaccgatct gcgtccctct gcgtcatctg cggatcgtct ctcaaatcag aatgcgtctg
     2281 caagcctgcg agcaatcgcg gcgtagccaa gggctcccgg atagaaatgg tcttcgaaag
     2341 attcaaggtt tggccgatgc gaacaccatt ttcgcggact gataaccagc cgttactgcg
     2401 atgatcacct gcgcgggtgg tggtgagccg gatgtgaccg cgttcaattg ttgccggtcg
     2461 aggggccaga tctcatagcg gccggccgga agatgcgtta gcaaaagccg gccttggccg
     2521 tcactgaaca tgggcaagcc ttggtaattg atcattcctt cccagacagg aatcggcagc
     2581 aggactccgt cgatgcggag cagcaccgcg acaccagtca acgcggcgcc ggatgtcgtc
     2641 tcgcaatcga tttcgagaga ggcgttacct tcagggaccc gaatgtggat atcgtctgct
     2701 cctttcccga gcgattcgaa ttgagcgaca ccgatggagc ctgatcttgg gacaacgaaa
     2761 accataccgt agtcttcggg gctgaccggc aataagatgc ggccgtcctc gtcggtagct
     2821 cctacaggat gcgttccgtt ccgagtgctt acgtatacgg ctgccgaaag tggcgcgccg
     2881 aacgagttcg tcacaacgac cggccggttt ccggccgcct tgagccgcac ttggggagac
     2941 tggtcggttg ccgtctcgtc gacggtgctc cacgaggtct gcaaggattg atatccactc
     3001 ttcgagacag cgagcgcgta gctgccggtc ggaaaggcgc ccagctcgaa gcgtccatcc
     3061 tctccgctcc gcagcgtgac cattgttgag cgatcgtcgt ttctcatcgt gacttcgacg
     3121 cgagcgtcgc ggatgggctg tccactggag tcgtccgtga cgcgcccaag aagccgccgg
     3181 ttcggaacgg agatagacca ccggttctta tcgccgtcga tgtgctctgt ctctcgccag
     3241 aatggaatga gtggcaactg cgtaacgatc gcggtatagg tcccctgttg ccagacctct
     3301 tccgtcacgc tccccccgct gtcagtcgtc acggaggaag tccaggagct atcctcgggt
     3361 tcaatgtgga ggatggctcc ggcgagtggc ttgtcgtcgt agcggacggt ggcctcgagc
     3421 accgctgcag agatctgtac gattgcctct gcctctcccc cttcggtcaa cgtcacgagt
     3481 tggccgattc gctgaagtgg attcgaccct gcgacgatca cgcggtattt gcctggttcg
     3541 agtgcgtcga caacggcctc cttttcaccc tttggaagtc gtccgctgtg cgcgagctcg
     3601 tcgccacgtg acccggcgag cggtcgtcgc acttcccagg ttacgtcttc ctgcccaagc
     3661 ggcggtgcga gcctgatggt cagcgatcca cctttggaga gacgtactga gagctcagta
     3721 tccgcaacgg ccttgggcag gagaacagtg cgcctcgcgt aaccggccat ggccacgcga
     3781 atgaactgcg gccacaagtc agcaacaggc gcgcgaaagt tacctttccg gtcggtcacc
     3841 gcaagcgagt tcccgttggg cagatacacg cgggcatctc gaagcggaat tgcggttttc
     3901 gcgtcgatga ccgttcccga caggatgggt attttctgca ggaacacatt gccaacctct
     3961 acgtcgcgca gttgccgctc gacacttcgg tatccgttgc ttttgaccag cagggtacag
     4021 ccgctgccag gatggcggag atccacgccc ggcaccggga cgcgcgtgac agaccacttc
     4081 caggtcaaat cctggcatcg aagctcaaaa tcgagggtcg ctcgcgtcga gccatccgac
     4141 agacgcgggg ccagtttatg gtgctgtgag gaagcgggaa gagtgagtac attggcgccg
     4201 gcctggatcg ttccggttgc gatctgcttg aggtccgagg agatccaaac gaaatcccgg
     4261 acagtgtccg aaggggcaac ggtacccggg atctcgttga ctgcctggca acgctgctca
     4321 atgcagaacg tagaaggcgc tgctgcaacg gtctgcaccg ccaaaaacgc ggcgatcacg
     4381 atcgtcacaa gctggaccgt ccggacggcc cgcgcctcag gatcacggac actagcttcc
     4441 actcgccgga acggtagggc agttgaggtc cacgcctatc ttcagctaag acgacaatgc
     4501 aaccggattt cgtggagcct gcgcaattgt ccgcgcccaa ataaccgtcg cattccccct
     4561 gcatgaggca caccgtcgta ttaaatggac cgaagtctcg caaggcgcac aagatagagg
     4621 cgttgtaaga agtggtttca cagtacg
//
LOCUS       DATLVW010000920         8405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_123993, whole genome shotgun sequence.
ACCESSION   DATLVW010000920 DATLVW010000000
VERSION     DATLVW010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123993"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VNN25_27300"
     CDS             <1..592
                     /locus_tag="VNN25_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXH95312.1"
                     /translation="FPLDMLTAWHMLVARAQLRMGETVLVHAGGSGVGSAAIQIAKLF
                     GATVYATAGTAEKASRAQALGADATIVYTETDFLDDVRRLTGKRGVDVVFEHVGGETF
                     ERSLKALARGGRLVTCGATTGGEATINLRLVFFKLLSILGSTMGSLAELHQIVKLVEQ
                     GRLHPVIDRVLPLAEVAEGHRILEAREAFGKIVFEV"
     gene            697..2472
                     /gene="mutL"
                     /locus_tag="VNN25_27305"
     CDS             697..2472
                     /gene="mutL"
                     /locus_tag="VNN25_27305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXH95313.1"
                     /translation="MPRIQLLPSDLINQIAAGEVVERPASVVKELVENAIDAGAMVID
                     VLLENGGKKLIEVRDNGSGMAHDDALLALERHATSKIRGFDDLAQVHTLGFRGEALPS
                     IASVSRFTLITSTGDEAIEIECDPLTGSRNSRPAARDRGTTISVRELFENVPARRKFL
                     KSADAEFRAIVTIVSSYALPLPSRAFRVEHNGRTVVDLPPAATMRERVIQIIGDEAAP
                     YLEEIDATIGAVHAAGYVTRGLRYGSRRNQYFFVNGRLVKDRVLTHAANRGAEAFDFD
                     GHPAIVLFVGVEPEFVDVNVHPAKTEVRFRDSSAVHVAVEQGIKRALGGAEEGSQLLQ
                     AAGPAPAAAPAPSTLFAPQRFDDAPAGYRPEFTPLFQRQSLVQPPLREQTALIESQPQ
                     LQQSEPQLGDLQGRVIGQYRMSYIVIDTPTGLRLIDQHVAHERVLYDRYFDRAAANAP
                     VSQQLLTPILYESGAAESAVLESHIEELRAVGFDMERFSGNVFAIASVPSELVKKDVD
                     AFLRKLVDASLDDRSHLQHLRDKVCASLACQAAIKVHRPLSGEEMARLTAELLDSSNP
                     YACPHGRPIIVDIRHLDIEKHFHRK"
     gene            2605..3000
                     /locus_tag="VNN25_27310"
     CDS             2605..3000
                     /locus_tag="VNN25_27310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXH95314.1"
                     /translation="MRIPSRQLGARGERRAAWFYRLRGYRIVARNVRLRAGEIDLIVR
                     RGGKLVFVEVKARQSLFAGHGVDAVDRRKQLQLITLASAYLGRHPHDGEIRYDVLSLF
                     WTGWRFVITHFPDAFRPVSDSRRPWRWRV"
     gene            complement(2963..3547)
                     /locus_tag="VNN25_27315"
     CDS             complement(2963..3547)
                     /locus_tag="VNN25_27315"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXH95315.1"
                     /translation="MASIRDEFAARGLEFAVCGGIAMAIHGFTRATEDLDLFVRPEEV
                     EAIEAALEGLGYAIKSAPATVGAMNIRRVSKIDAAEDDNLAVDLLLVSPETSAVWNSR
                     EVVSWRDQPLPVVSREGLIALKRLRATSMDLVDIGRMETEGDDTSRPISLCIGRVAQL
                     RNFCLSLGKAGRAARKKGQLSPLTPATSKVSSNR"
     gene            complement(3571..6252)
                     /gene="alaS"
                     /locus_tag="VNN25_27320"
     CDS             complement(3571..6252)
                     /gene="alaS"
                     /locus_tag="VNN25_27320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXH95316.1"
                     /translation="MLTTNQIRQQFLEYFEKNQHRRVSSSSLVPAGDPTLLFTNAGMN
                     QFKDVFLGREPRDYKRATTSQKCVRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYF
                     KEDAIKFALDLIVNVWGLEVERLWFSVFEGGDGVPADEEAAALWVKAGASPDRVLRFG
                     KKDNFWSMGDTGPCGPCSEIHYFRGDDLSKNVAALVNGEGDDTMEIWNLVFMQYDRGA
                     DGVLTPLPAPSVDTGMGLERLASVLQKTRTNYDIDLFKPIIRDVQSLSGYDYGQPQHE
                     ELNVATRVLCDHSRAMTFLVADGVIPSNEGRGYVLRRIIRRAVRFSRKFYIKFRPSKL
                     VFSVVESMKDAYPELVARQEMVMKTLEAEEDRFERTLSTGMERVGQILDDIRARGERT
                     IGGAALFRLYDTFGVPVDLIGEMAEEEGVTLDREGFETMMADARVRAKASSKFQLSAG
                     AAVFAPIAEKTGPTEFVGYDQYVDVPSTVKAIVVAGVEQDAIHHGAEGEVVLSPTPFY
                     AESGGQIGDSGALEWDGGRATVLDTQKPASDLIVSRVRVDEGHLTLHSKVRASVPVPT
                     RLDTTANHTATHLLHQALKDVLGETVQQAGSLVAPDRLRFDYTYHQAPTEVQLRKVEE
                     IVNDKIRANLDVTKTVMPIAEAKKTGAVAMFGEKYGENVRIVTAGDFSREFCGGCHVN
                     RTGDIGLFKMVSERSLAAGVRRMEALTGRGALAYFQTIEDAAHELQQQLNVKLEDVPA
                     QLRTMQERQKSLEKELKQLKMRVATGGGASASSSNGEESVDIDGVALVARRFDDVSGG
                     DLRNLADTFRSKIKSGVVVLGSVADGKVTLLTAVTKDLLDRVQANTLINKLAPIVGGK
                     GGGKPDLAQAGGKDPDKLDEAIAQAPHALRELLGARA"
     gene            6349..7155
                     /locus_tag="VNN25_27325"
     CDS             6349..7155
                     /locus_tag="VNN25_27325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXH95317.1"
                     /translation="MFWVLLLIALTISPARNPPRIIILGYHEVEEDGLPPHEVIPRAG
                     ATATPDEMALYTISASALQQQLDALDRHGYEVIPLADVVDYIAGRRSALPRRSAVITV
                     DDGWRSTTTVIAPELAKRNWPFTAFVYPQAIDAHSHHPYNLTWSEVAGLTMEGADIES
                     HTYSHPLLSRAHASVADESNYEQFLDDELRCSRATIEEHINQRVRFLAYPYGDYDAGV
                     IAAAKAAGYDAAVTVTPGPVTRQSNPLALDRYLVFHDTTLQQFEAWLEGP"
     gene            complement(7152..7688)
                     /locus_tag="VNN25_27330"
     CDS             complement(7152..7688)
                     /locus_tag="VNN25_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXH95318.1"
                     /translation="MISGRPLDGEFAAYAKTDIDYVAGDDAVGALAAQGEEVAVLLES
                     LDEEATRAWRYAPGKWTVKEVFGHLIDDERIFAYRALCVARGDARPLPGFEENEYVAA
                     TNFESRPLASLISEYRAVRAGSLTLFESLTAEEWTRRGNVNGYEASVRGLAFHMAGHE
                     LHHLRTLRERYELFASVR"
     gene            complement(7798..8280)
                     /locus_tag="VNN25_27335"
     CDS             complement(7798..8280)
                     /locus_tag="VNN25_27335"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXH95319.1"
                     /translation="MSGLRPDVDLNACYLAAMRILNHRFNSEAELQRKLALKEFDAEV
                     ISATIEKLRREKWLDDDRFAASFVRARVRKGLGRHRITRELSAAGVESELIARALDES
                     VPDHDERAAALASARKRLAVLRRRDDDDAKIREKLVAYLFRQGYDGSLAVDVVRELMT
                     "
BASE COUNT         1522 a         2817 c         2477 g         1589 t
ORIGIN      
        1 gtttccactc gacatgctca ccgcctggca catgctcgtt gcccgcgccc agctccgcat
       61 gggcgagacc gtcctcgttc acgccggcgg ttccggcgtc gggagcgccg ccattcagat
      121 cgccaagctg tttggcgcga ccgtttacgc caccgccgga accgccgaga aagcctcccg
      181 tgcccaagcc ctcggcgccg acgccaccat cgtctacacc gagaccgact ttctcgatga
      241 cgtgcgccgc ctgaccggca agcgcggcgt cgatgtcgtc ttcgagcacg tcggcggcga
      301 gacgttcgag cgctcgctga aagccctggc gcgcggcggc cgcctcgtca catgcggcgc
      361 cacgacgggc ggcgaagcaa ccatcaatct ccgtctcgtt ttcttcaagc tcctctcaat
      421 cctcggctcc acgatgggca gcctggccga gttgcaccag atcgtgaagc tcgtcgagca
      481 gggccgcctc cacccggtga tcgaccgcgt cctcccccta gccgaagtcg ccgaaggaca
      541 tcgcatcctc gaagcgaggg aagcgttcgg gaagatcgtg ttcgaggtgt gatccgcaga
      601 tgacgcagat gatcgcagat cattttctta tctgggttcc atctgcgtca tctgcggatc
      661 gttttctcga ccgcgaaacc gcgagataat ccgcaagtgc cccgcatcca acttctcccc
      721 tccgatctca ttaaccagat cgctgccggc gaggtcgtgg agcggccggc atcagtcgtc
      781 aaggagcttg tcgagaatgc catcgacgcc ggcgcgatgg tcatcgacgt cctgctcgaa
      841 aacggcggca agaaactgat cgaggtgcgc gacaacggct cggggatggc gcacgacgat
      901 gccctcctcg ccctcgagcg tcacgccaca tcgaagattc gcggcttcga cgatctcgcg
      961 caggtacaca cgctcgggtt ccgcggcgaa gcgcttccct cgatcgccag cgtctcccga
     1021 ttcacgctca tcacctcgac gggcgatgaa gcgatcgaga tcgaatgcga tccgctcacc
     1081 ggctcgcgga attcgcgccc cgctgcgcgc gaccgcggca cgaccatcag cgtgcgcgag
     1141 ctgttcgaga acgtcccggc gcggcgcaag ttcctcaagt ccgccgacgc cgagttccgc
     1201 gccatcgtca cgatcgtctc gtcgtacgcg ctgccgcttc cgtcgcgcgc cttccgcgtc
     1261 gagcacaacg ggcgcacggt cgtcgatctc ccgccggcgg cgacgatgcg cgagcgcgtc
     1321 atccagatca tcggcgacga agcggcaccg tacctcgagg agatcgacgc cacgatcggt
     1381 gccgtccacg ccgccggcta cgtcacgcgc ggcctccgct acggcagccg gcgcaatcag
     1441 tatttcttcg tcaacggccg gctggtgaag gaccgcgtcc tgacgcatgc ggcgaatcgt
     1501 ggcgccgagg cgttcgactt cgatgggcat ccagcgatcg tgctttttgt gggcgtcgaa
     1561 ccggagtttg tcgatgtgaa cgtgcatccg gcgaagacgg aagtgcgttt ccgcgattcc
     1621 agcgccgttc atgtcgcggt cgagcaaggc atcaaacgcg ccctcggcgg cgcagaagaa
     1681 ggctcgcaac tgctgcaagc tgccggtccg gctcccgcag ctgcacctgc gccgtcaaca
     1741 ctctttgcac cgcagcggtt cgatgacgcg cccgccggtt accggcccga gttcacgccg
     1801 ctctttcaac gacagagcct cgtgcagcca cccctccgcg agcagactgc gctgatcgag
     1861 tcgcagccgc agttgcagca gagcgagccg cagttgggcg atttgcaggg acgcgtcatc
     1921 ggccaatacc ggatgagcta catcgtcatc gacacgccca ccggtctgcg tctcatcgat
     1981 cagcatgtcg ctcacgagcg cgttctgtac gaccgctact tcgaccgcgc cgccgcaaac
     2041 gcgcccgtga gccagcagct tcttacgccg attctttacg agagcggggc cgcggaaagt
     2101 gcggtgctcg aatcgcacat cgaggagctg cgcgcggtcg gtttcgacat ggagcgcttt
     2161 tccgggaatg tcttcgccat cgcctccgtc ccgtcggagc tcgtgaagaa agacgtcgat
     2221 gccttcctcc gcaaactcgt tgacgcctcg ctagacgacc gctcgcacct ccagcatctc
     2281 cgcgataagg tctgcgcctc cctcgcctgc caggccgcga tcaaggttca tcgtccgctc
     2341 agcggcgagg agatggcccg cctcaccgcc gagctcctcg actcgtcgaa cccctacgcc
     2401 tgcccccacg gccgcccgat catcgtcgac atccgccacc tcgacatcga aaagcacttc
     2461 caccgcaagt agaaccggtt tcaccactga gacacagagg acacagagag ctctctgtga
     2521 tctctgtgtc tctgtggtga gtctttccac ctccggtaga acttcgatgc gtttcgaatc
     2581 atccgaggga tcctcggcag cgacatgcgc atcccgtcac gacaactcgg cgcacgcggc
     2641 gagcggcgcg ctgcatggtt ctaccggctg cgcggctacc gcatcgtcgc gcgcaacgta
     2701 cgtctgcgcg ccggcgagat cgacctcatc gtccggcgcg gcgggaagct ggtcttcgtc
     2761 gaagtgaagg cgcggcaaag tctgtttgca ggacatggcg tcgatgccgt cgatcgccgc
     2821 aagcagctcc aactcatcac cctagccagc gcatacctcg gccgacatcc gcacgacggc
     2881 gagatccgct acgacgttct ctccctcttc tggaccggct ggcgtttcgt catcacgcac
     2941 tttccggatg cgttccgccc ggtcagcgat tcgaggagac cttggaggtg gcgggtgtga
     3001 gtggcgacaa ctgacctttc tttcgcgccg cgcgtcccgc cttcccaagc gacaggcaaa
     3061 aattgcgcag ttgcgctacg cgtccgatgc agagcgagat tggcctgctg gtgtcatcgc
     3121 cttcggtctc catccgcccg atatcgacga gatccatgct cgttgcccgg agacgtttca
     3181 acgcgatcag cccttcgcgc gatacgacag gcaacggttg gtcgcgccag ctcacgacct
     3241 cgcggctgtt ccaaacggcg cttgtttccg gagacacgag caacagatcc acagcaaggt
     3301 tgtcgtcctc ggctgcgtcg atctttgaaa cacgccggat gttcattgcg ccaaccgtag
     3361 ccggggccga cttgatcgca tagccgagcc cttccagcgc tgcttcgatt gcttcgactt
     3421 cctccggcct cacgaagaga tcaaggtcct cggtcgcgcg cgtgaaaccg tgaatggcca
     3481 tggcgattcc gccgcagaca gcgaactcga gacctcgggc agcaaactcg tctcggatgg
     3541 aagccaactc ctccgagaga tcgaacacgc ctacgccctc gcccccagca actcccgcag
     3601 cgcgtgcggc gcttgcgcga tcgcctcatc cagcttgtcc gggtccttcc cgccggcctg
     3661 cgcgagatcg ggcttgccgc cgcccttgcc gccgacgatc ggggcgagtt tattgatgag
     3721 cgtgttggcc tggacgcggt cgagcagatc cttcgtcacc gcggtcagca gcgtgacctt
     3781 cccgtcggcg acggagccga ggacgacgac gccggacttg atcttcgagc ggaacgtgtc
     3841 ggcaagattg cgcaggtcgc cgccgctgac gtcgtcgaaa cggcgggcga ccagcgccac
     3901 gccatcgatg tcgaccgact cctcgccgtt tgacgacgag gccgacgctc cgcctcccgt
     3961 ggctacgcgc atcttgagtt gcttgagctc cttctccagg ctcttctgcc gctcctgcat
     4021 cgtccgcaac tgcgccggga cgtcttccag cttcacgttg agctgctgct gcaactcgtg
     4081 cgcggcgtct tcgatcgtct ggaagtacgc gagcgcgccg cggcccgtga gcgcttccat
     4141 acgccgcacg ccggcggcaa gcgaacgttc gctgaccatc ttgaacaggc cgatgtcgcc
     4201 ggtgcgattg acgtggcagc cgccgcagaa ctcgcgcgag aaatcgcccg cggtcacgat
     4261 gcgtacgttc tcgccgtact tctcgccgaa catcgccacc gcgccggtct tcttcgcctc
     4321 ggcgatcggc atgaccgtct tcgtgacgtc gaggttcgcg cggattttgt cgttgacgat
     4381 ctcttcgacc ttgcgcagct gcacttccgt cggcgcctgg tgatacgtgt aatcgaagcg
     4441 cagccggtcc ggcgcgacga gcgagccggc ctgctgcacc gtctcgccga gtacgtcttt
     4501 cagcgcttga tgcagcagat gtgtcgccgt gtgattcgcg gtcgtgtcga gccgcgtcgg
     4561 caccggtacg gatgcgcgca ctttcgaatg gagcgtgaga tgtccttcgt ctacgcgaac
     4621 gcgcgacacg atgagatcgc tggccggctt ctgcgtgtca agcaccgtgg cccggccgcc
     4681 atcccactcc agcgcgccgc tgtcaccgat ctgaccgcct gactccgcgt agaacggcgt
     4741 cggggaaaga acgacctcac cttccgcgcc gtggtgaatg gcgtcctgct cgaccccggc
     4801 aacgacgatc gccttcaccg tcgagggaac gtcaacatac tgatcgtagc caacgaactc
     4861 cgtcggcccg gtcttctccg cgatcggcgc gaagactgcg gcgccggccg acaactggaa
     4921 cttcgacgac gccttggcgc gaacgcgcgc gtccgccatc atcgtctcga agccttcgcg
     4981 gtcgagagtg actccttcct cttccgccat ttctccgatg agatcaactg ggacgccaaa
     5041 ggtgtcgtac aagcgaaaga gtgccgcgcc accgattgtt cgttctcccc tggcgcgaat
     5101 gtcgtcgagg atctggccaa cacgctccat accggtggag agagttcgtt cgaaacgatc
     5161 ctcttccgcc tctagagtct tcatgaccat ctcttggcgt gccacaagtt ccggatacgc
     5221 gtccttcata gactcaacca ctgaaaaaac cagcttggat ggacggaatt tgatatagaa
     5281 cttgcgacta aatcgaactg cccgacgaat aatgcgtcgc aatacgtagc cgcggccttc
     5341 atttgacgga atgacaccgt cggcaacaag aaaagtcatt gcgcgcgaat gatcgcagag
     5401 cactcgagta gcgacgttga gctcttcgtg ctgaggctgg ccgtagtcgt atccgctcag
     5461 cgattgaacg tcacggatga tcggtttgaa caaatcaatg tcgtaattcg tcctcgtctt
     5521 ctgtaacacg ctggcgagcc gctccagccc catgcctgta tcgaccgagg gtgccggaag
     5581 cggcgtaagt acgccgtcgg caccgcggtc gtactgcatg aagaccaggt tccagatctc
     5641 catcgtgtcg tcgccctcgc cgttgacgag cgcagcaacg ttcttcgaca gatcgtctcc
     5701 gcgaaagtaa tggatctcgg agcagggccc gcacggaccg gtatccccca tcgaccagaa
     5761 gttgtccttc ttgccgaagc gcagcacgcg gtccggcgac gcgcccgctt tcacccaaag
     5821 cgcggccgcc tcttcgtcgg caggaacgcc gtcgccacct tcgaagacgg agaaccagag
     5881 ccgctccact tcaaggcccc agacgttgac gatgaggtcg agggcgaact tgatcgcatc
     5941 ctctttgaag tagtcgccga acgagaagtt gccgagcatc tcgaagaacg tgtgatgccg
     6001 cgccgtgcgg ccgacgttct ccagatcgtt gtgcttgccg ccggcgcgga cgcacttctg
     6061 cgacgtcgtg gcccgcttgt agtcgcgcgg ctcgcgcccg aggaaaacat ctttgaactg
     6121 attcatgccg gcgttcgtga aaaggagcgt cgggtcgccc gccggaacga gcgacgagct
     6181 cgacacgcgt cggtgctgat tcttctcgaa gtactcgaga aactgctggc ggatctgatt
     6241 ggtcgtgagc aaaaaggact ctcctcgaaa gcgcgagatc gtaacagccg aggttgggcg
     6301 tgtcatcccg atgaggtttc tggcattcct gttgattccg agaacggcat gttctgggtt
     6361 ctcctgctca tcgcgctcac catttccccg gcgcgaaatc cacctcgcat catcatcctc
     6421 ggctaccacg aagtcgaaga ggacggcctg ccgccgcatg aagtcattcc gcgtgccgga
     6481 gcaactgcga cacctgacga gatggcgctc tacacgatca gcgcctcggc gctccagcaa
     6541 cagctcgacg cgctcgaccg gcacggttac gaagtgatcc cgcttgcgga tgtcgtcgat
     6601 tacattgccg ggcggcgatc agcgctcccg cggcgctcgg ccgtgatcac cgtcgatgac
     6661 ggatggcgct cgaccacaac ggtgattgct ccggagctgg ccaaacgcaa ttggccgttc
     6721 acggcgttcg tctatccgca ggcgatcgac gcccactcgc atcatccgta caacctgacg
     6781 tggtccgagg tcgccggcct gacgatggag ggagccgata tcgagagcca cacctattct
     6841 catcctctcc tcagccgcgc tcacgcctcg gtcgccgacg aatcgaatta cgagcagttc
     6901 ctcgatgacg aacttcgctg cagccgcgcc acaattgagg agcacatcaa tcagcgggtg
     6961 aggttcctcg catacccgta cggcgattac gacgccggcg tgatcgctgc ggcgaaggcc
     7021 gccggctacg acgccgcggt gaccgtcacc cctggtccgg tgacgaggca atcgaatccc
     7081 ctcgcgctcg atcgatacct ggtatttcac gacacgacgt tgcagcagtt cgaagcgtgg
     7141 ttggaggggc cctagcgaac gcttgcgaag agctcatatc gctcccgcag cgttcgcaga
     7201 tgatgcagct cgtgacctgc catgtggaac gcaagtccgc gcaccgaggc ttcgtagccg
     7261 ttcacgttac cgcggcgcgt ccactcctcg gcggtcagcg attcgaaaag agtgagtgac
     7321 ccggcgcgga cggcccggta ttcagagatg agagaagcga gagggcgtga ctcgaagttg
     7381 gtcgctgcga cgtattcatt ctcctcgaat cccggcaacg gtcgcgcatc tcctcgggcc
     7441 acacaaagcg cgcgatacgc gaagatgcgc tcgtcgtcga tgagatggcc gaacacttcc
     7501 ttgaccgtcc atttgcccgg cgcataccgc cacgcgcgag tcgcttcttc gtcaagcgat
     7561 tcgagtaaca cggcgacctc ttcgccttgc gctgccagcg ctccgaccgc gtcgtcgccg
     7621 gcgacgtaat cgatgtcggt cttcgcgtac gcggcgaact caccatccaa cggccgtcca
     7681 ctgatcatga ttggtcccct catttcgcgc aaagcgcttt ggactgggtg gcgccagcca
     7741 ccgcttttga agtgtgcgaa tcgagctgac tccccattcc gtctcttcaa agatgatcta
     7801 cgtcatcagc tcgcgaacca cgtcgaccgc aagactgccg tcatagccct ggcggaagag
     7861 atatgccacg agtttctcgc ggatctttgc atcgtcatca tcgcgccggc gaagaacggc
     7921 tagccgtttt cgcgctgacg ccagcgccgc ggcgcgctca tcgtgatcgg gaacgctttc
     7981 gtccagcgcg cgggcgatca attcgctctc cacgccggcg gcggacaatt cgcgggtgat
     8041 gcgatggcgc ccgaggcctt tgcgaacgcg ggcgcgaacg aaggaggcgg cgaaacgatc
     8101 atcgtcgagc cacttctcgc ggcggagttt ctcgatcgtc gcgctgatca cctcggcatc
     8161 aaactctttc agcgccagct ttcgctgcag ctccgcctcg ctgttgaagc gatggttgag
     8221 gatgcgcatt gcggcgaggt agcaggcatt gagatccacg tcaggcctca acccgctcat
     8281 cgcggctgaa gattctcagg tcggggcgaa agctggcaga ccagaacttc ggcgcgcagc
     8341 aagggaacac cgggaggatc gaagttcacg cgccgtacgg ccatcgattc gagcaacgcg
     8401 cgggc
//
LOCUS       DATLVW010000921         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_124018, whole genome shotgun sequence.
ACCESSION   DATLVW010000921 DATLVW010000000
VERSION     DATLVW010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124018"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VNN25_27340"
     CDS             complement(<1..942)
                     /locus_tag="VNN25_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HXH95320.1"
                     /translation="MDRRRFLASAASLGIMGVWATRSAAAPSRVVWREDRASYPQGVA
                     SGDPDDHSVILWTRRPFDQGERQSLTLEVARDADFRRVVAKARAPVLAAADWTCRALV
                     GGLSPATTYWYRFTDDSGAGSRIGRTITAPRASDPRPVRFAFVSCNSVNEGAQNAYRR
                     MIWEDERAPADRKLGFVLHLGDFIYEVVEYPDEVSHRYARNVYDLGRIPDDRKVGNFH
                     VPTNLAGYRHVYRAHINDPDIQDARAHFPFVCIGDNHEFSWQGWQSFITYGGNKIEPA
                     QPLRVAANQAWWEYIPSRVRKASGPGLDQFGPPAVTIA"
     gene            1477..>2185
                     /locus_tag="VNN25_27345"
     CDS             1477..>2185
                     /locus_tag="VNN25_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXH95321.1"
                     /translation="MFRTILKSAAFLFVAAAVVAGAQEKTATDSLKSGFMNPPPSARP
                     RVWWHWMNGNITKEGIKLDLDWMHQIGLAGFQNFDAALQTPQVVDHRLAYMTPEWKEA
                     FKYAIGLGRQYGMEMAIAGSPGWSETGGPWVPASEGMKKYVWSETLVEGGKPFSGALK
                     HPPSRTGAFQDIGIHDELGATDQGKAPQFYADAAVFAYKRGPVDVSVEDLNAKITSNG
                     DSLDPAILSDGDLEKTTA"
BASE COUNT          450 a          648 c          676 g          411 t
ORIGIN      
        1 tgcgatggtc accgccggcg ggccgaactg gtcgagtcca gggcccgagg ccttgcggac
       61 gcgcgacggg atatattccc accacgcctg attggctgcc acgcgcagcg gctgcgcggg
      121 ctcgatctta ttgccgccgt atgtgatgaa gctctgccag ccttgccacg agaattcgtg
      181 gttgtcgccg atgcacacga agggaaaatg cgcgcgcgca tcctggatgt caggatcgtt
      241 gatatgcgcg cgatagacat ggcgatagcc ggcgaggttg gtcggcacgt ggaaattgcc
      301 cactttgcgg tcgtcgggaa tgcggccaag gtcgtagacg ttgcgcgcat agcggtgcga
      361 cacctcgtcc ggatattcaa ccacttcgta gatgaagtcg cccagatgga gaacgaagcc
      421 cagcttgcga tcggccggcg cgcgctcgtc ctcccagatc atccggcgat aggcgttctg
      481 cgcgccttcg ttgacgctgt tgcacgacac aaaggcgaag cggacggggc gcgggtcgct
      541 ggcacgcggg gcagtgatag tgcgcccgat tctgctgccg gcgccgctgt cgtcagtgaa
      601 gcggtaccaa taggtcgtcg ctggtgagag gccgccgacc agcgcgcggc aggtccagtc
      661 ggccgccgca agcaccggcg cgcgtgcctt cgcaacaact ctgcggaaat ccgcatctcg
      721 cgccacttcc aaagtcagtg actgcctctc gccctggtcg aacggccgac gggtccacaa
      781 gatcacgctg tgatcgtccg gatcgccgga tgcgacaccc tggggataag acgcacgatc
      841 ctcccgccat acgactcgcg acggcgcggc agccgatcgc gtcgcccaca cgcccatgat
      901 tcccagagaa gcagcacttg ccagaaatcg ccgtcgatcc atctcacgct ccctatttga
      961 aggctatcgt ttcataacca ccactggtca aaactggact caccctagca gtcgcaaaaa
     1021 tcgcgtgtaa agctattctt gacgaaacgg tctctggggg ggatgaagta cggccacgat
     1081 ctccgcaatc gcggctaccc ttgctcgccg aagtgttccc caggacggag gcttctcgca
     1141 ggcctgaagg tcatagttgt cggaggccat ccggacgatc cggaatcttt cgcggaaggg
     1201 tccatctctc gagtccggac atgatcttcg cgccggtggc ctgtcgattc ccttgaattc
     1261 tcgatgcagg ccggggtgga tcccgttccc cagggctggt gtcgaatagc tctccgatgc
     1321 cggtgcccgt aatacacagc agatagttgg acagttccca accgctgttg tcggggatga
     1381 agtgaagctg acaatagacc tcgatgtcgc taagcagtag actcaagttc gcaaaaagtt
     1441 tccgctctca tcgccgagcg agtcgaaagg agaagtatgt ttcgtacgat cctcaaatca
     1501 gccgcgttct tgttcgttgc agccgcagtt gtagccggcg cgcaggaaaa gacggcgacc
     1561 gattccctga agagcggttt catgaatccc cctcccagcg ccaggccgcg cgtgtggtgg
     1621 cactggatga acgggaacat caccaaggaa ggtatcaagc tggacctgga ctggatgcat
     1681 cagatcggac tggccggctt ccagaacttc gatgccgcgt tgcagacccc gcaggttgtc
     1741 gaccaccggc tggcttatat gactcctgag tggaaggagg cgttcaagta cgcgatcgga
     1801 ttgggccggc agtacgggat ggaaatggcg attgcgggtt cgcccggatg gagcgagacc
     1861 ggcggcccgt gggttccagc ctcagaggga atgaaaaaat acgtgtggag cgagacgctc
     1921 gtggaaggcg gcaaaccgtt ttcaggcgcg ctcaaacatc cgccttcgcg aaccggtgca
     1981 ttccaggaca taggaatcca cgatgagctt ggagcgaccg atcagggcaa ggcaccgcag
     2041 ttctacgcgg atgccgccgt cttcgcttac aagagaggcc ccgttgacgt gtcagtcgag
     2101 gatctgaatg cgaagatcac ctcgaatgga gactccctcg acccagcaat actctcggac
     2161 ggcgatctcg agaagactac cgcgg
//
LOCUS       DATLVW010000922         5389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_409526, whole genome shotgun sequence.
ACCESSION   DATLVW010000922 DATLVW010000000
VERSION     DATLVW010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409526"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VNN25_27350"
     CDS             complement(<1..412)
                     /locus_tag="VNN25_27350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95322.1"
                     /translation="MATTKPESRNAFINVPYAKRYEGVYLAFIAGIVGHGLVPTAAVR
                     DPSSRYQLERIYELISAAQYSFHDLSWMSLDGKAPRTPRLNMAFELGLAVALSRAEGA
                     NHQWFVFDTVPYRLDKALSDLGGIRPRIHDRSPES"
     gene            complement(424..636)
                     /locus_tag="VNN25_27355"
     CDS             complement(424..636)
                     /locus_tag="VNN25_27355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95323.1"
                     /translation="MSPKIIITTPFPTPEEVAAYYRIPAERVAELDRMIAEHRAEAVA
                     GPHKKASSRNGGRNASKVVKASRSKK"
     gene            complement(682..1800)
                     /gene="bshA"
                     /locus_tag="VNN25_27360"
     CDS             complement(682..1800)
                     /gene="bshA"
                     /locus_tag="VNN25_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HXH95324.1"
                     /translation="MRIGITCYPTFGGSGVVATELGIALARGGDDVHFISYQMPSRLS
                     VLHERVRFHEVTVTPYPLFDPYPPYTLALATKMAEVADYEKLDILHVHYAIPHAISAH
                     LAREILSSRKLHFVTTLHGTDITLVGRDPSYLPITRFGIEVSDGVTAVSEWLRVQTAR
                     NFGTSKDIEVIPNFVDPARFRKHDSALSHLFSSSGEKLLCHVSNFRPVKRIMDVLAVF
                     ERVTRIIPSRLVMIGDGPERSVAEAFCRDHGLREKVFFLGNVPNLEEVVGAADVFLLP
                     SEAESFGMAALEAMASEVPVIGSRAGGLPEVVVEGETGYLLPVGDIEGMAGRAVEILS
                     SPDLQRRLGRNGRDLAEGKFNVNRVVPRYREFYERVIG"
     gene            complement(1828..2532)
                     /gene="bshB1"
                     /locus_tag="VNN25_27365"
     CDS             complement(1828..2532)
                     /gene="bshB1"
                     /locus_tag="VNN25_27365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HXH95325.1"
                     /translation="MIFFGAHPDDIELSCGATIAKAVKDGLRVGIIELTRGELGTRGT
                     PKVRRREALKAAGILGARFREQLDFGDGGMRTGRDQEMQIIAAVRLCKPKVVFTMWPD
                     DRHPDHIRTGQIVAQASFLSGLRALTTDLPAHRPQVTIYYPQNYLVTPSFVVDVTKTW
                     KTKMRAIAAFKSQFYDPKSKEPPTFIADKKFLEMIEARGRHFGALIGVEYGEAFMTKQ
                     PPKVDDIIAAYEGREP"
     gene            complement(2575..4122)
                     /gene="bshC"
                     /locus_tag="VNN25_27370"
     CDS             complement(2575..4122)
                     /gene="bshC"
                     /locus_tag="VNN25_27370"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HXH95326.1"
                     /translation="MSSVKTEPPCLCFPLANYPGLSKFTSDWLAGNENATQFLKREPQ
                     LVEHRRPAGWTAAGTAATLVSALAESNRTWNHNVDDELARWSTGETFTVIAGQQVGFA
                     GGPIYTLAKLAGMLKLKRVNEAKGIPTTVFFWLATEDHDFAEVATLALTKRDPKQQLD
                     LTTLRAHKHDSRRVVGAMPIPEELTRSLIEYLDIPRPAWLREGITFRDSFAELLTSAV
                     DEKFILVDALLPELRRAGQPLFDGILKNWDGIQGELAQRSKALENAGYAPQVIPRDGE
                     PYTLLFELDEHGRRELLDGPRPIASPERTSTSALTRPLLQDFVLRPDLFVGGPSEVAY
                     YAQIAPLHELLDVPMPRVALRAHELLAPKRVVRTIARFDIQPEEIFAGADALLASREP
                     AGVSEVEQLASEARRALIEKIVRIGEIALPAEHALARAINRSIGHIEYHFDKLTERAI
                     RGLARKDRERYAAVRDLVTTLVPDGSPQDRVTGWFGYWNEHGRHLIDRMIEEAAPDED
                     VCKVVGL"
     gene            complement(4119..4832)
                     /locus_tag="VNN25_27375"
     CDS             complement(4119..4832)
                     /locus_tag="VNN25_27375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXH95327.1"
                     /translation="MTWYREWFGEEYLELYAHRDEHEARRQVLFFQEHFGRVDGVILD
                     LACGMGRHLTEFNGAGFDAVGCDLSFTLLRTGIDEYGPLPVARADMRSLPFCSGSFAG
                     LVNFFTSFGYFATEEENLSVVREMARVLRPDGVFLFDYLNVDRELEKLVERETRDTPS
                     GNVEIERWFDPRDRSFNKRITIGQKRYLERVRGYDLNEISTMFTSSGLSIRQVFGDFD
                     GAPFDAASPRLILVGSKAR"
     gene            complement(4950..>5389)
                     /locus_tag="VNN25_27380"
     CDS             complement(4950..>5389)
                     /locus_tag="VNN25_27380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95328.1"
                     /translation="MTADATYANNNLSALKIRGGYNGTAVQGSPRLDWEANITYAYDT
                     NDRLTKEELALTSMTKTYTERPYGAERQEIGTLYQDMRVRKPVEHMLSTGDRCGISGT
                     QVLGNPVDLRPFYALTPNLAIVLGNGVTRASVSFTYPDSYRVR"
BASE COUNT         1001 a         1601 c         1745 g         1042 t
ORIGIN      
        1 cactttccgg tgatcggtcg tggatgcgcg gccgtatccc tccgagatcg ctgagcgcct
       61 tgtcgagacg atagggaacg gtatcgaaca cgaaccactg atgatttgcg ccctcagctc
      121 ggctcaaagc aaccgcaagg ccgagctcga acgccatgtt cagacgcggt gtccgaggag
      181 ctttcccatc caacgacatc catgaaaggt catgaaatga gtattgagcg gcagagatca
      241 gctcataaat ccgctcgagt tgatagcgac ttgaaggatc gcgaacagcc gccgttggca
      301 ccagaccatg cccaacgatt ccagcgatga acgccaagta gacaccctcg taccgtttcg
      361 cgtaaggcac attgataaag gcgttccgcg attccggctt ggtcgtggcc accgcgacac
      421 cactcacttc tttgagcgcg aagccttaac gaccttcgaa gcgtttcgac ccccgttccg
      481 tgaagaggcc ttcttgtgcg gaccggctac cgcctccgcg cgatgctcgg cgatcatccg
      541 gtctagttcg gcgacccgct ccgcggggat ccggtaatac gcggctacct cctcgggcgt
      601 ggggaacggc gtggtgatga tgattttggg ggacatgatc gtgactattc taacgccgag
      661 cgcggatcgc gcgttccctc atcacccaat caccctctcg taaaactccc gataccgcgg
      721 cacgacccgg ttgacgttga acttcccttc ggccaaatcc cgcccgttgc ggccgaggcg
      781 gcgttggagg tcgggagagg agaggatctc gacagcgcga ccggccatcc cttcgatgtc
      841 gccgactggg aggaggtagc cggtctcgcc ttctacgacg acttctggta atcctccggc
      901 acggctgccg atgacgggga cttccgaggc catcgcttcc agggccgcca tcccgaacga
      961 ctcggcctcg gacggcagga ggaagacgtc tgctgcgccg acgacttcct cgaggttcgg
     1021 cacgttgccg aggaagaaga ctttctcgcg gaggccgtga tcgcggcaga acgcctcggc
     1081 gaccgagcgc tccgggccgt cgccgatcat gaccagacgg ctcggaatga tgcgcgtgac
     1141 acgctcgaag actgccagca cgtccatgat ccgtttcacg ggacggaagt tcgaaacgtg
     1201 gcagagcagc ttttcgccag acgacgaaaa taaatgcgag agcgcgctgt cgtgtttgcg
     1261 gaatcgtgcg ggatcgacaa agttcgggat gacctcgatg tctttgctcg tgccgaaatt
     1321 gcgcgccgtc tggacgcgca gccactcaga cacggcagtc acgccgtccg agacttcgat
     1381 tccgaaccgc gtgatcggaa ggtagctcgg atcgcggcct acgagcgtga tgtccgtccc
     1441 gtgaagcgtg gtaacaaagt gcagcttgcg tgacgaaagg atctcgcgcg ccaggtgggc
     1501 gctgatggcg tgggggatcg cgtagtgaac gtgaaggatg tccagctttt cgtaatccgc
     1561 gacctccgcc atcttcgtcg ccagcgccag cgtgtagggt ggatacggat cgaagagcgg
     1621 gtacggtgtg accgtgacct catgaaagcg cacacgctcg tggagcacgc tgaggcgcga
     1681 cggcatctgg taactgatga agtgaacgtc atcgccgccg cgcgccagcg cgatgccgag
     1741 ctccgtggcc acgacgccgg agccgccgaa tgtggggtag caggtgatgc cgattctcat
     1801 ggcagtctcg tggtcgccga ctcgaagtta cggctctctc ccttcatacg ccgcgatgat
     1861 gtcgtcgacc ttcggcggct gtttggtcat gaacgcttcg ccgtactcga ccccgatgag
     1921 tgcgccgaag tggcgtccgc gcgcttcgat catctcgagg aacttcttgt cggcgatgaa
     1981 cgtgggcggt tccttcgact tgggatcgta gaactgcgat ttgaaggcgg cgatggcgcg
     2041 catcttcgtc ttccaggtct tcgtcacgtc gacgacgaag ctcggcgtga cgaggtagtt
     2101 ctgcgggtag tagatcgtca cttgcggccg gtgcgcgggc aggtctgtcg tcagcgcgcg
     2161 aagtcccgac agaaatgagg cttgcgcgac gatctgtccc gtccggatgt ggtcgggatg
     2221 gcggtcgtcg ggccacatcg taaaaacgac cttcggtttg cagaggcgga cggcggcgat
     2281 gatctgcatc tcctgatcgc ggccggtgcg catgccgccg tctccgaagt cgagttgctc
     2341 gcggaaacgg gcgcccagga tgccggctgc tttgagcgcc tcgcggcggc gaaccttggg
     2401 agtgccgcgc gttcccagct caccgcgcgt cagttcgatg atgccgacgc gcagtccgtc
     2461 cttcaccgct ttcgcaatcg tcgcgccgca cgaaagctcg atgtcatcgg gatgcgctcc
     2521 gaagaagatc atatcgaccg gttcaagcaa tggatcgccc ccatttcata cgcttcacaa
     2581 acccaccacc ttgcacacgt cctcatccgg cgccgcttct tcgatcatcc gatcgatgag
     2641 gtgccggccg tgctcgttcc agtagccgaa ccacccggtt acgcggtctt gcggcgaacc
     2701 gtcggggacg agcgtcgtca ccagatcgcg gaccgcggcg taccgttccc ggtctttgcg
     2761 cgccaggccg cggatggcgc gctcggtcag cttgtcgaag tgatactcga tatggccgat
     2821 cgagcgattg atggcgcgcg ccagtgcatg ttcagcggga agtgcgatct cgccgatgcg
     2881 gacaatcttc tcgatgagtg cacgcctcgc ttcggaggcg agctgctcaa cctcgctcac
     2941 gccggccggc tcgcgcgacg ccagtaaagc gtcggcgccg gcgaagatct cctccggctg
     3001 aatgtcgaag cgcgcgatcg tgcggacaac gcgcttcggc gcgagcagct cgtgcgcacg
     3061 aagagcgacg cgcggcatcg ggacatcgag cagctcgtgc agcggggcga tctgcgcgta
     3121 atacgccacc tcggaaggtc cgccaacgaa gagatcgggg cgaagaacaa aatcctgcag
     3181 gagcgggcga gtcagcgcgg aggtcgatgt tcgttccggc gaagcaatcg gtcgcggacc
     3241 atcgagaagc tcgcgcctgc catgttcatc cagctcgaag agcagcgtgt acggttcgcc
     3301 gtcgcgtgga atgacctgcg gcgcgtagcc ggcgttctcc aatgccttgg aacgttgcgc
     3361 aagctcgccc tggatgccgt cccaattttt caggatgccg tcgaacagtg gctggcctgc
     3421 gcggcgcagc tcggggagaa gcgcatcgac gaggatgaat ttctcgtcga ccgcggacgt
     3481 gagcagctct gcgaaggagt cgcgaaaggt gattccttcg cgcaaccagg cggggcgggg
     3541 gatgtcgagg tattcgataa ggctgcgcgt caactcttcg ggtatcggca tcgcgccgac
     3601 gacgcgtctc gagtcgtgct tgtgcgcgcg tagcgttgtc agatccaact gctgcttcgg
     3661 atcgcgtttc gtcagcgcca gcgtcgctac ctcggcgaag tcgtgatctt cagtggccag
     3721 ccagaagaag acggttgtcg ggatgccttt cgcttcgttg acgcgcttca gcttgagcat
     3781 gccggcgagc ttggcgagtg tgtagatcgg accacctgcg aagccgacct gctggccggc
     3841 gatgactgtg aaggtttcgc cggtggacca acgggccagc tcgtcatcga cgttgtggtt
     3901 ccaagtgcgg ttcgattcgg cgagagcgct gacgagggtc gccgccgtgc cggcggcggt
     3961 ccagccggcg ggccgccggt gctccaccaa ttggggctcg cgctttagga actgtgttgc
     4021 gttttcattg ccggctagcc agtcgctcgt gaatttgctg aggccggggt agttcgccag
     4081 cggaaagcag agacagggag gttccgtctt cacgctgctc atcgggcttt gctcccgacg
     4141 aggatgaggc ggggcgaggc tgcgtcgaac ggcgcgccgt caaagtcgcc gaagacttgg
     4201 cggatcgaaa ggcccgagga agtgaacatc gtcgagatct cattgagatc gtaaccgcga
     4261 acgcgctcca ggtaacgctt ttgaccgatc gtgatccgtt tgttgaacga acggtcacgt
     4321 ggatcgaacc agcgctcgat ctccacgttg ccgctgggcg tatcacgcgt ctcgcgctcg
     4381 acaagctttt cgagctcgcg gtcgacgttg agatagtcaa acaggaaaac gccgtcgggc
     4441 cgcagcacgc gcgccatctc gcgcaccacg ctcagattct cctcttccgt cgcgaagtag
     4501 ccgaagctcg tgaagaagtt gaccagaccg gcgaagcttc cgctgcagaa ggggagcgag
     4561 cgcatgtccg cccgagcgac cggaagcggc ccatattcgt cgatgccggt gcgcagcagg
     4621 gtgaaggaaa gatcgcagcc gacggcgtcg aagccggcgc cgttgaattc cgtgagatga
     4681 cggcccatgc cgcaggcaag atcgagaatg actccgtcga cgcggccgaa gtgttcctgg
     4741 aagaagagca cctgacgcct ggcttcgtgc tcgtcgcgat gcgcgtagag ctcgaggtac
     4801 tcctcgccga accactcgcg ataccaggtc atgtcagcga gagatcaggg cagaagagat
     4861 cagggcagca gagatcagag acaagagatc ggagatcagg aaggctgttc tgatctctag
     4921 gctctgatct cttcatcgct ttcccacttc tagcgcactc tgtacgaatc cggatacgtg
     4981 aagctcacgc tcgcgcgcgt gacgccgttg ccgaggacga tggcaaggtt cggggtgagc
     5041 gcgtagaacg ggcgaaggtc gactggatta cccagaacct gcgtgccgga aatgccgcaa
     5101 cgatcgccgg tgctgagcat gtgctcgacc ggcttgcgta cgcgcatgtc ctggtagagc
     5161 gttccgatct cctgacgctc tgcgccgtac gggcgctcgg tgtaggtctt ggtcatcgag
     5221 gtgagtgcga gctcttcttt cgtcaatcga tcgttggtgt cgtaggcata ggtgatgttc
     5281 gcttcccagt cgaggcgagg cgatccctgc actgcggtgc cgttataacc gccgcggatc
     5341 ttgagagccg acaggttgtt gttcgcgtat gtggcatcgg cggtcatgg
//
LOCUS       DATLVW010000923         9380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_535892, whole genome shotgun sequence.
ACCESSION   DATLVW010000923 DATLVW010000000
VERSION     DATLVW010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535892"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VNN25_27385"
     CDS             <1..1494
                     /locus_tag="VNN25_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95329.1"
                     /translation="MVGGVSPFEAGAAWIWIRSAGVWTQQGPPLVGSGAAGSASFGSS
                     VSLSSDANTAAVGGFTDNNYAGAVWIWTRSGGVWTQQGTKLVSPESANAQQGRGVALS
                     ADGNLLIIGGPNDLTQTGGIWFWTRSAGVWAQRGSKIVGSGATGHAFQGISVSLATDG
                     DTALVGGSMDSNGHGATWVFVAAPDLIVTKSHTGTFKQGDNGDTYTITVSNLGLKPTS
                     GTVTVIDTVPVGLTPTAPTGSFDGWTCSINGQVLTCTQNDVLQSGASYAPITLTVNVA
                     NDASPIVTNTATVSGGGELNTANDAASDVTPIHAEFAPVTPTGVAATAVTTTQINITW
                     LAVNGAASYEIDRKAAGSGFSQIGTSAANSFSDTTPSADSAYLYRVRAVNEAGKSSSS
                     AADLATTVIFVDDPLLNSLVKAVHLSQLRTAANAVRLLAGLTAFSFTDTSTAGTPIRA
                     LHLTELRSSLDEARAPLGLTTGGYTDASPANVAIKTVHFQELRSRVQ"
     gene            1931..3607
                     /locus_tag="VNN25_27390"
     CDS             1931..3607
                     /locus_tag="VNN25_27390"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HXH95330.1"
                     /translation="MKLRRRIALLVIACAILCVPEAVLAQYTQQGPKLVGSGAVGNAE
                     QGISVAISGDGNTAIVGGILDDIQIGAAWVWTRSGGGVWTQQGPKLLASDAPRNITFV
                     GAEQGYSVALSADGNTAIVGGPQDGAHGAAWIWTRSGGVWTQQGLKLVGSGAVGDASQ
                     GWSVALSADGNTAIVGGDTDNDDHGAAWVWTRSGGVWTQQGAKLVGSGAVSRFGFEDQ
                     GSSVSLSADGNTALIGGSNDNDGVGAAWVWTRSGGVWTQQGGKLVGPGVGQSNEGISV
                     SLAADGNTAIIGGTLDPSQNSGFTGAAWVWVRSGGVWNQVGKLVGAGNAVSLSADGQS
                     AAVGGASGGVGGTWVWTRSGSAWVQSAKLVGTGAVDPANQGRSVSISADGKTVITGGN
                     DDDFVTGAAWVFAATADLSVTKSVTGGPSFAAGGNLNYSIHVVNNGPGSASSVVMTDV
                     VPSGSTFVSATSSQGSCSGTTTVTCTVGTLANGGGATISLVLKVPSAPGTVSNTASVT
                     ALELDPDPTNNSSTATVKTIDPNLIPVASSWVIVALAGMLALVGTMKMRG"
     gene            complement(3809..6127)
                     /locus_tag="VNN25_27395"
     CDS             complement(3809..6127)
                     /locus_tag="VNN25_27395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH95331.1"
                     /translation="MGGPDRRMRLLGLGAVGVAVFAIGVFVALRQLPEWRNLSIPEES
                     FFAARLQQIAGPADLRLESLPHVQLRSNGILLESDLLSARETAYDVLGPSAADWLARE
                     GRGPFVDATAQTRWAGGSERGQLRVVFSVRGVPISAIWIPEELLRSRPSASSAHLELD
                     RMFLPPGQTVPTTELTVLTETLRLAAMPASAPPEVLITTSIRGADAPTARRVVGTVGA
                     QRGRLENLTFGSFLTARMESLIVRAIFWIAVLVLFGLLLIRRRIELKKGLVLGALSIA
                     LSLPGALGNSASWILVVQDLGTVTSKALALFFLWSAAESWLRSTVPGFRTSLDTLRAG
                     RLGPKGGTALLAGWSIGASVAGLWLLAMSFGTMVPGVATNDGSIRMPFFGVATSPIEE
                     GAFRTGLIMLVVCIALRWPLLRRIRGGATLLGGLVLATRIPLSSFGIAFTAGLLLTAI
                     LVYAYAEFGLTALLTAAMMSAVLPAALYSLLHFYWVPLASVLLLTVAIAPVPLGLLGI
                     RRDDEAEEGPLPLPDFARRLEEENRLKYEMDLLARMQLGLLPKEMPQIEGYEIVASSI
                     LASEAGGDLYDFVRDSAGRLWIAAGDVSGHGYSCAIAQAMTKAGLASLVEAERTPAMV
                     LQRLDLVLREIGSPRTFTSLALLRLDPITGEALVSNAGHPYPWIVRGTDVRELDLPSL
                     PLGLGPAREYTDTPLSIPTGTTIVIFSDGLFEAHDTHARPYGFDRLREILGKVSRRPA
                     SAILTAILEDWRRHAGPATPADDTTVVAVKRT"
     gene            6308..8008
                     /locus_tag="VNN25_27400"
     CDS             6308..8008
                     /locus_tag="VNN25_27400"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXH95332.1"
                     /translation="MSESDTRGKWTRRRFLEAVGRVGGAAAVYESMVALGIVRVPDSA
                     YAKTLQIPEGAGNGKTIVILGAGVAGLTAAYELTKRKSGYNIIILEAQHRTGGRSYTV
                     RTGDKIIEKHGNDIWEQTSHFDKDQYLNAGPGRLPYHHTVALGYCRELKVAVEPYVMS
                     SRANLYQTQGSYRDKSVPNRRILNDTRGYIAELLTKSIDKGSLNDELSPAQQKQMLDL
                     LNTFGPLSNKKYIGSQRSGYTTEPGVTTPGDIEPPLKLIELLGSEFWLDRMYQPEDYE
                     WQPTLFQPVGGMDMLANGFLPFVKDFIRTNREVTYVHNVAGNKVEIHHRTAGDPSSAV
                     ETITADWCISTIPVWILAKDVDNNFSKPFRAALKAAKRGLTCKVGWQANRRFWELDNE
                     IYGGISYINHNITQMWYPSNGYLFEQKGVLTGAYNYTTRAEKLGNESLGERLKTAAAG
                     AHRLHGDTFTRDVPIDLGLSIAWHHVPYQLGGWADWEGVAPVHYETLLAPEGHVIVAG
                     DQVSYLPGWQEGAMLSAHHVLDQIIPRESRPKLKAAPAAKRRAPSTRAMTEGSGRTRE
                     "
     gene            8143..>9380
                     /locus_tag="VNN25_27405"
     CDS             8143..>9380
                     /locus_tag="VNN25_27405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXH95333.1"
                     /translation="MSELRRDIGLWRGIALNMIDMVGIGPFITIPLILAAMGGGRGML
                     AWILGGLLAISDGLVTAELSASIPGSGGSYVFLRHAYGPAGRLISFLFLFQILFSAPL
                     SMASGCIGFANYLRVVLPAAGNHVALTATIVCVLTTALLLRRIEAIGKFSILLWAGVL
                     ATLAIVIGAGLPHLRLDAFAFWRAPRLDGGIGYAGLGVALIFAVYDYLGYYNICYIGD
                     EVREPERTIPRVIVISILAIGVIYLVMNACLISVLPMKQAMVSPSIVADYLAVLLGTR
                     AAQWISILILWTAFASIFSLMLGYSRILYAAARDGNFFRFFGKLHATEAYPYVSILFL
                     GCVAAAFCSLSLKNVLQAILSIRAVIPFMAQIVGAVLLRQREPDRPRPFRMWLYPLPA
                     IIALGLWGYIVTSPQKGLKAG"
BASE COUNT         1853 a         2992 c         2910 g         1625 t
ORIGIN      
        1 atggttggag gcgttagtcc cttcgaagct ggcgcggcgt ggatctggat caggagcgcg
       61 ggagtgtgga cccagcaagg cccaccactc gtcggctcgg gagccgcagg gtcagccagc
      121 ttcggttcct cggtttcgct ctcgtccgat gccaatacag ccgccgtggg agggttcaca
      181 gacaacaatt acgccggagc cgtctggatc tggaccagga gcggcggagt gtggacccaa
      241 caaggaacga agcttgtcag cccggaatct gcgaacgctc agcaaggtcg cggcgtagcc
      301 ctttccgcag acggcaacct gctgatcatc ggaggcccaa acgacctcac ccaaactggt
      361 ggcatctggt tttggacgag aagcgcagga gtatgggcgc agcgcggatc gaagatcgtt
      421 ggctcgggag cgaccggaca cgcctttcag ggcatcagcg tgtctctggc caccgatggc
      481 gacaccgccc tcgtgggagg atcgatggac agcaatggcc acggcgccac ctgggtcttc
      541 gtcgctgcac ccgatctcat cgtcacgaag agccacaccg gaaccttcaa gcagggcgac
      601 aacggagaca cctacacgat caccgtgtcg aacctcggac tcaaacctac gtccggaaca
      661 gtgacggtca ttgacacggt gcccgtcgga ctgacgccaa cagcacccac cggatcattc
      721 gatggctgga cctgttcgat caacggacaa gtactgactt gtacgcagaa tgacgtgctc
      781 cagtcgggag cgagctacgc cccaatcact ctcacggtga acgtcgcgaa cgatgcctcg
      841 ccaatcgtga cgaacacagc taccgtctca gggggcggag agctcaacac cgccaacgac
      901 gccgccagcg atgtaacccc aattcatgcg gaattcgccc cggtgacccc cacgggagtt
      961 gccgccaccg ctgtcacgac tacgcagatc aatatcacct ggctcgcggt caatggagcc
     1021 gcctcatacg agatcgaccg caaagcggcc gggagtggct tctcgcagat cggcacgtcg
     1081 gcagcgaaca gcttctccga caccacgccg tccgccgatt ccgcgtatct gtaccgcgtt
     1141 cgcgcggtca atgaagcggg gaagtcctcg agcagcgccg ccgatctagc tacgacggtc
     1201 atcttcgtgg acgacccact gctgaacagc ctcgtgaaag cggttcatct ctcgcagctt
     1261 cgaactgcgg ccaacgccgt gcggttgctg gccggtttga ccgcattttc tttcaccgac
     1321 acgagcacgg cgggaacgcc gatccgcgca ttgcacctca ccgaacttcg ttcgagcctg
     1381 gacgaagccc gtgccccgct cggtctcacg acgggcggct acacggacgc atcgccggca
     1441 aacgtggcaa tcaagacggt ccactttcag gagctgcgca gtcgcgttca gtagggtcgc
     1501 cggcgagccg ccggcggtac agccgccggg ccggcggcgc tacgcttggc ctactactcg
     1561 ccgctacgca gttcgtttca caaccatgat gttcgtgtca tccgccgctt cggtcgtgat
     1621 acggattggg gaactgaaac acgaaccatc gtaagaaaag ggagcgaagc gaagcgtagc
     1681 gccgacggct cgtcggctgt atcgccaccg gctcggtggc ggttcacaag gtcgccgggg
     1741 ccggacgacg aacgtgcaga cgcggcaagg cgacacgcca tccgcgcggg tgctatcggc
     1801 aagtgcgcgt gcaccgcagc ggctacacgg atgcgttcgc tggcaaattg gcaattaaag
     1861 cgtcccacta cgttaagagc tcacagagtg tgtcaagatc actctcaaat cccgttcaac
     1921 cgaggctccc atgaaactgc gccgccgcat tgcgctattg gtcatcgctt gtgcaattct
     1981 ctgtgtcccc gaagcagtcc tcgcgcaata cacgcagcag gggccgaagt tggtcggttc
     2041 aggggcggtg ggcaacgctg aacaaggcat ttcagtcgcc atctccggcg acggcaatac
     2101 ggcgatcgtt ggaggaattc tcgacgatat tcaaatcggg gcggcgtggg tctggaccag
     2161 gagcggcggc ggcgtctgga cgcagcaggg tcccaagctg ctcgcctcag acgctccgcg
     2221 gaacatcacc tttgtcggcg cggaacaagg ctactccgtg gctctctcgg ccgacggcaa
     2281 tacagccatt gttggaggcc ctcaggatgg agcccatggc gctgcgtgga tctggaccag
     2341 gagtggaggc gtctggacgc agcagggact gaagctggtc ggctcgggcg ctgtcggtga
     2401 cgcgagtcag ggatggtccg tggccctctc agcagatgga aacacggcca tcgttggcgg
     2461 cgataccgac aacgatgacc atggggcagc ctgggtttgg acccgcagtg gaggagtatg
     2521 gacccagcag ggagccaagc tggtgggctc gggcgccgtg agccgattcg gattcgaaga
     2581 tcaaggtagt tccgtttcgc tctccgctga tggcaacacg gcactcatcg gaggatcgaa
     2641 cgacaacgat ggcgttggag ccgcgtgggt ctggacgagg agcggaggag tatggaccca
     2701 gcagggaggc aagctggtcg gccccggcgt gggacaatca aatgaaggca tatctgtgtc
     2761 actcgccgca gatggcaata cagcaatcat cggtggcact ctcgatccct cgcagaactc
     2821 tggatttacc ggcgcagcgt gggtctgggt caggagcgga ggggtctgga atcaagtcgg
     2881 aaagctcgtt ggcgcgggaa acgccgtctc cctttctgct gatggacaga gcgcagctgt
     2941 tggaggcgct tccggcggcg tggggggcac atgggtctgg acgaggagcg gaagcgcctg
     3001 ggtccagtcc gctaagcttg tcgggacggg cgccgtcgac ccggccaacc aaggcaggtc
     3061 cgtatcgatc tccgccgacg gaaagacagt catcaccgga ggcaacgacg acgacttcgt
     3121 gactggcgcg gcgtgggtat tcgccgcaac ggcggatctg agcgtcacca agtccgtcac
     3181 cggagggcca tccttcgctg ccggcggcaa cctcaattac agcatccatg tcgtcaacaa
     3241 cggacctggc agcgcaagca gcgtggtcat gacggatgtg gttccctccg gatcaacgtt
     3301 cgtatccgcg acgtcgtcgc agggatcgtg cagcggaacg acgacagtga cctgcacagt
     3361 cggaacactg gcaaacggcg ggggtgccac gatcagcctt gtactgaagg ttccttctgc
     3421 gccgggcacg gtttccaata cggcttcggt tactgcgttg gagctcgacc ctgatccaac
     3481 aaacaactcc tccacagcga cggtcaagac gatcgacccg aacctcattc cagtagcctc
     3541 gagctgggtg attgttgcgc tcgccggaat gctcgcgttg gttggaacga tgaaaatgcg
     3601 gggttgattg ttcaacgaga tgacataacg gccattcgcg tcagccttta gtactcacga
     3661 gcagagttgt ggacgtatcc cgatttcgca ctttcaaaat ggggtcaggt ctgcttttga
     3721 aagtgcccat gggcatcaag aagacgaaaa catgtggtca ctttcaaaag tggacctgac
     3781 cccattttga aagcgccgcg tgggcaacct acgtccgttt caccgccaca accgtcgtgt
     3841 catccgccgg cgttgccgga cctgcgtggc ggcgccagtc ttcgaggatc gcggtgagga
     3901 ttgcggaagc cgggcggcgg gagactttgc ccaggatttc gcggaggcgg tcgaagccgt
     3961 aggggcgggc gtgggtgtcg tgcgcttcga acaggccgtc ggagaagatg acgatcgtcg
     4021 tgccggtggg gatggacagc ggcgtatccg tgtattcgcg cgcggggccg agtccgaggg
     4081 ggagcgacgg gagatcgagc tcgcgaacgt cggtgccgcg aacgatccag ggatacgggt
     4141 gaccggcgtt gctgacgagc gcttcgccgg tgattggatc gaggcggaga agcgccagcg
     4201 atgtgaacgt tcttggcgag ccgatctcgc gcagcacgag gtcgaggcgt tgcaggacca
     4261 tcgccggagt tcgttccgct tcgacgagac tggccagccc tgccttcgtc atcgcctgcg
     4321 cgattgcgca ggagtagccg tgaccggaga cgtcgcccgc cgcgatccaa agcctgccgg
     4381 cggagtcacg cacgaagtcg tagagatcgc cgccggcttc gctcgcaagg atcgagctgg
     4441 ccacgatctc gtagccttcg atctgcggca tctccttcgg caggaggccg agctgcatgc
     4501 gcgccagcag atccatctcg tacttcagcc ggttttcttc ctccagtcgc cgcgcaaagt
     4561 cgggcagagg aagcggtccc tcttccgctt cgtcgtcgcg gcggatgccg agaaggccga
     4621 gggggaccgg ggctatggcc acggtgagga gcagcaccga cgccagtggc acccagtaga
     4681 aatgaagcag cgagtagagc gccgcgggaa gaaccgcaga catcatcgcc gcggtgagta
     4741 gcgcggtgag tccgaactcc gcgtacgcgt aaaccagaat cgccgtgaga agaaggcccg
     4801 cggtaaacgc gattccgaac gacgagagcg gaatgcgcgt ggccagaacc agtccgccga
     4861 ggagcgtcgc gccgccgcgg atgcgccgga gaagcggcca gcgcaacgcg atgcagacga
     4921 caagcatgat caggccggtg cgaaacgccc cttcctcgat cggcgacgtg gccacgccga
     4981 agaacggcat ccggatacta ccgtcgttcg ttgccacgcc cggcaccatc gttccgaacg
     5041 acatcgccag cagccagagg ccggcgaccg atgcgccgat cgaccagccg gcgagcagcg
     5101 ccgttccacc tttcggcccg aggcgtcccg cgcgaagcgt gtcgagcgac gtgcggaagc
     5161 cgggcacagt cgagcgcagc cacgactccg cggccgacca gaggaagaac aacgccaatg
     5221 ccttgctcgt gaccgtgccg agatcctgca caaccaggat ccacgaagcg gagttgccca
     5281 acgctccggg aagcgacagc gcaatcgaaa gcgcgccgag aacgaggcct ttcttcagtt
     5341 cgatccgccg gcggatcaga aggaggccga agagcaccaa aaccgcgatc cagaaaatcg
     5401 cgcgaacgat cagcgattcc atgcgcgcgg tcaggaagga tccgaacgtc aggttctcca
     5461 gccgcccccg ctgcgcgccg accgtaccga cgacgcgcct cgcagtgggc gcgtccgccc
     5521 ctcgtatcga cgtcgtgatg agaacttccg gcggcgcaga tgccggcatg gccgccagcc
     5581 gtaacgtctc ggtcagaacg gtcagttcag tcgtaggaac cgtctggccc ggtgggagaa
     5641 acatcctgtc cagttccagg tgcgccgaag aagcggacgg tcgtgaccgc agcaactctt
     5701 ccggaatcca gatggcggag atcggaacac cgcgcacgga gaaaacgacg cgcaactgcc
     5761 cacgctcgct gccgcctgcc catcgagttt gtgcagtcgc gtcgacgaac ggcccgcggc
     5821 cttcccgcgc cagccagtcg gcggccgatg ggccgagaac gtcgtacgcc gtctcgcgcg
     5881 cggaaagaag atcgctctcc aggaggatac cgttcgatcg cagctgcacg tgcggcagcg
     5941 attcgagccg gagatcggcg ggaccggcga tctgttgcag cctcgccgcg aagaaactct
     6001 cctcggggat gctcagattg cgccactccg gaagctggcg cagggcgacg aagacgccaa
     6061 tcgcgaacac cgcaactccg acggcaccaa gcccgaggag tcgcatgcgc cggtcaggtc
     6121 cacccattca gatcaaggta tacagcgaac aggaggaaaa gttccagcgc gactacctgg
     6181 atcagacgga tccgacggat cggaccgatc agacggatcg agcaagcgca ccacccccat
     6241 tgtccattct cgattaaccc gctacgctta cgtcatccca gaattcacgt gagacgcgga
     6301 ggcaccgatg agcgaatcgg atactcgtgg caagtggact cgcaggcgtt ttctcgaagc
     6361 ggttgggcgc gtcggcggcg ctgccgctgt ttacgaatcg atggtcgcgc tcgggatcgt
     6421 gcgcgtgccc gactctgcgt atgcgaagac gctgcagatt ccggaaggcg ccggcaacgg
     6481 taagaccatc gtcatcctcg gcgccggtgt ggccggactc accgccgcgt acgagctgac
     6541 caagcggaag agcggctaca acatcatcat cctcgaagcg cagcatcgca ccggcggacg
     6601 cagctacacc gtccgcaccg gcgacaagat catcgagaaa catgggaacg acatctggga
     6661 gcagacgtcc catttcgaca aggatcagta cctcaacgcc ggaccgggac ggcttcctta
     6721 ccaccacacc gtcgcactcg gatattgcag ggagttgaag gtcgcggtcg agccgtacgt
     6781 catgtcgtcg cgcgccaacc tctatcaaac gcaaggctcg tatcgcgaca agtccgtccc
     6841 caaccgccgc atcctcaacg acacgcgcgg ctacatcgcc gagctgctaa cgaagtcgat
     6901 cgacaagggc tcgctcaatg atgagctctc gcctgcgcag cagaagcaga tgctcgatct
     6961 gctcaacacg ttcggaccgc tgagcaacaa gaaatacatc ggctcgcagc gctccggcta
     7021 cacgaccgag ccgggagtga cgacgccggg cgacatcgag ccgcccctca agctcatcga
     7081 gctcctcggc tccgagttct ggctcgaccg catgtaccag ccggaagact acgaatggca
     7141 gccgacgctc ttccagccgg tcggcggcat ggacatgctg gcgaatggtt tcctgccgtt
     7201 cgtcaaggac ttcatccgca ccaatcgcga agtgacctac gtccacaacg tcgcaggcaa
     7261 caaggtcgag attcaccatc gcacggccgg cgatccgtca tccgcggtcg aaacgatcac
     7321 cgccgactgg tgcatctcga cgatcccggt ctggatcctc gccaaagacg tcgacaacaa
     7381 cttctcgaaa ccgttccgcg ccgcactcaa ggcggccaaa cgcggtctga cgtgcaaggt
     7441 gggatggcaa gccaaccggc gtttctggga gctggacaac gagatctacg gcggcatctc
     7501 gtacatcaat cacaacatca cgcagatgtg gtacccgtcg aacggctacc ttttcgaaca
     7561 gaagggtgtg ctgacgggcg cgtacaacta cacaacgcgc gccgaaaaac tcggcaacga
     7621 atcacttggc gaacggctga agaccgctgc cgccggtgcg catcggttgc acggcgacac
     7681 cttcaccagg gatgttccta tcgacctcgg cctctccatc gcctggcatc acgtccccta
     7741 tcaactgggc ggctgggcgg attgggaggg cgtcgcaccc gtacattacg agacgcttct
     7801 tgcgcccgag ggacacgtga tcgtcgccgg cgaccaggtg tcgtacttgc ccggatggca
     7861 ggaaggcgca atgctctccg cgcaccacgt gctcgatcag atcattccgc gcgaatcgcg
     7921 tccgaagctg aaagccgccc ccgccgcaaa gcgccgcgcc ccaagcaccc gcgccatgac
     7981 cgaaggctca ggccgcacgc gcgagtaacg accacttctc acagatctgt caatcgagcc
     8041 ccttctcccc atcgcttctt gatggggaga aggtggccga aggccggatg aggggccgtc
     8101 cactctctcg acaacgttgc cgccatgtag actccctttc caatgtctga gctgcggcgc
     8161 gacatcggac tgtggcgcgg gatcgccctg aacatgatcg acatggtcgg gatcggtccg
     8221 ttcatcacga tcccgctcat cctggcggcc atgggcggcg gccgcggaat gctggcttgg
     8281 atcctcggcg gcctgctcgc catcagcgac ggtctcgtca ccgccgagct ctccgcgtcc
     8341 atccccggtt ccggcggctc gtacgtcttt ctccgtcatg cctacggtcc cgccgggcgg
     8401 ctcatctcct ttctcttcct cttccagatc ctcttctcgg cgccgctgtc gatggcatcc
     8461 ggctgcatcg gtttcgcgaa ctacctgcgc gtcgtccttc ccgccgccgg aaaccacgtc
     8521 gcgctcaccg ccacgatcgt ctgcgtgctg acgacggcgc ttcttctgcg ccgcatcgaa
     8581 gcgatcggca agttctcgat cctgttgtgg gctggcgtgc tggcgacatt ggcgatcgtc
     8641 atcggcgcgg gcttgccgca ccttcgcctc gatgcattcg cgttctggcg cgcaccgcgt
     8701 cttgacggcg gcatcggcta cgcgggcctc ggcgtcgcgc tcatcttcgc cgtctacgac
     8761 tacctcggct actacaacat ctgctacatc ggcgacgaag tccgcgagcc cgagcggacg
     8821 attcctcgcg tgatcgtgat ctcgatcctg gccatcggcg tgatctatct cgtcatgaat
     8881 gcctgtctca tctccgtgct gccgatgaag caggccatgg tctccccatc gatcgtcgcc
     8941 gactacctgg ccgtcctcct cggcacgcgc gccgcgcaat ggatctcgat cctcattctc
     9001 tggaccgcct tcgcctcgat cttctcgctg atgctcggct actcgcgcat cctctacgcc
     9061 gctgcgagag acggaaactt cttccgcttc ttcggtaaac tgcacgccac cgaggcgtat
     9121 ccgtacgtgt cgatattgtt cctgggatgc gtggccgccg cgttttgctc gctgtcgttg
     9181 aagaacgtgc tgcaagcgat cctttcgatt cgcgccgtga ttcccttcat ggcgcagatc
     9241 gtcggcgcgg tacttttgcg gcaacgcgag ccagatcgtc cgcgcccatt ccgcatgtgg
     9301 ctctatccgc tccccgccat catcgccctc ggactgtggg gctacatcgt gacgtcgcca
     9361 caaaaaggat tgaaagcggg
//
LOCUS       DATLVW010000924         4711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_288631, whole genome shotgun sequence.
ACCESSION   DATLVW010000924 DATLVW010000000
VERSION     DATLVW010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288631"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..897)
                     /locus_tag="VNN25_27410"
     CDS             complement(133..897)
                     /locus_tag="VNN25_27410"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXH95334.1"
                     /translation="MDAPIRLIVTADDVGLHPGMTAGAIRAHREGIVTACSVVASGAA
                     FDDAVSQLKSVPLLEVGVHLALVEERSLTGMRLPKSYRSFVLGSKDFAAIERELRAQV
                     ERVLASGLRVTHLNGHQHLHMLPSIFAIVVRLAKEYGIGYVRRVLDRGGRGGPVRRAS
                     IAALNRLGRKAAAPRGNDITIGVMEAGHLTAARIVALLQHAEGTTELVTHPGIGVDAY
                     QHWRYAWDLETAALCDRSVREAIANRGIELIAPSQV"
     gene            complement(913..1839)
                     /locus_tag="VNN25_27415"
     CDS             complement(913..1839)
                     /locus_tag="VNN25_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXH95335.1"
                     /translation="MRATTQLIRATSQRVRLTSRAINRTAMKTKPSLFGIIACPNSIM
                     RTPSVSLVIPMFNEALNIEHAMTAAVEACTKYTDDYEIIIVDDASTDQSPFLVACRAA
                     ENPRIRMIRHERNRKLGGSLKTGFAAAKKDLVLYMDADLPFDPDALGRAMRAMAVTGA
                     DLIAGYRLDRTLEGPRRTLYSYLYNGLIGVLFGWPHRDINFSFKLMKREVLDAVELKS
                     EGSLIDAELIVKAKNRGFSIQQIGLDYFPRIRGESHLSSPRVILKIFAELWRLYPEMR
                     VVVSAEEVERVVREKRSAAGDVHVADRQPLET"
     gene            2580..3569
                     /locus_tag="VNN25_27420"
     CDS             2580..3569
                     /locus_tag="VNN25_27420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95336.1"
                     /translation="MRFGVCWQPRVWHGWLPPIPTLASWQAVQAAGITDLRLTYDPAL
                     PTGLFDTDLRNAHAAGLRINVNMGAVWESGGAPIDPDPAIIEAQAFDLATRFGDMIAL
                     YSFDNEPGAIAQTYEAANPGSDFMQDAYMPACTAFVRGIRRVAPDAIIGGCDADSVDI
                     QSRYIELADALLPVVVCDRRRVHPYGDVGGARYATMAGDDENDGFAQATDPRPLDVGE
                     IDHQQLPGATGIARPSDLQALLAFTRMIHAKYPTVGQIDYGTAEYFFTRLPASGIVPA
                     TTWSTWTFGPEPVVSDAGRQFTQLFHTINAAQPSADATAIAPPVFGGDPIVIQ"
     gene            complement(3563..>4711)
                     /locus_tag="VNN25_27425"
     CDS             complement(3563..>4711)
                     /locus_tag="VNN25_27425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95337.1"
                     /translation="GFNALRLYETKQMQYVAAHDASVSRGGMSTAMLVVRFIFHPWGW
                     KYVTLPLFACVTLGVAAFARLLRRDKVRSTLLPFIAFVIVQVTFELGWMDPADGARYS
                     LAFMPFIALLAALGFGVIRRSAGFAAAPWAVTAILAAATLVFVWPLAGTRTKLASPPA
                     AAAAWASANLAPNSVLGYDIALKPHAEYLMSRFRLLPVDKAIAEAYDRPGTGLVLLAN
                     GGAHGADSHVFAWPESDAYRKLTRGLYQEVTIENVRAAERYLPLSGAYPIERTLEGTE
                     WRWLGPDATIRLPQSHGATASLTLRIPGDLPYTADDVTISINGGDSAVLHVGREAVVA
                     NLELPSDPRIDVRLRSSRSFQPSTLMHNQDPRTLAVQLLHVETGTQLH"
BASE COUNT          887 a         1523 c         1480 g          821 t
ORIGIN      
        1 ccctctccgg gtacgagacg acgacatcgg agctgagcag ggctgttcaa gtgcggtgat
       61 gcagagatca gaaagacaga gatcagagat cagaaccccc tatctctgat ctcttgtctc
      121 tgatctctgc cgtcaaacct gcgaaggcgc aatcaactca atcccgcgat tggcaatcgc
      181 ctcgcgcacg ctccgatcgc agagcgccgc ggtctccaga tcccacgcat agcgccagtg
      241 ctgataggca tcgacgccga tgccgggatg cgtgacgagt tccgtggttc cctcggcgtg
      301 ctgcaacagc gcgacgattc gtgcggcggt gagatgcccc gcctccatga ctccgatcgt
      361 gatgtcgttg ccgcgcggag ccgccgcttt ccgcccgagc cggttcagtg ccgcgatcga
      421 tgcccttcgc accggccctc cccgcccgcc ccgatctaac acgcggcgca cgtagccaat
      481 cccgtactcc ttcgccagcc ggacgacgat ggcgaagatc gagggcagca tatgcagatg
      541 ctgatggccg ttgaggtgcg tcacgcgcag cccgctggcg agaacgcgct cgacctgcgc
      601 ccgcagctcg cgctcgatcg cggcgaagtc cttgctgccg aggacgaagc tgcgatagga
      661 cttcggtaag cgcatgcccg tcagcgaacg ctcttcgacc agcgccagat gaacgccgac
      721 ctcgagcaat ggcacgctct tcaactgcga caccgcgtca tcgaatgcgg cgccgctcgc
      781 gacaacgctg caagcggtca cgatgccttc acgatgcgcg cggatcgctc ccgccgtcat
      841 cccgggatgc agcccgacat cgtcggcggt gacgatgaga cggatcggtg cgtccacagg
      901 agtcacgcga agctacgtct cgagcggctg gcggtccgct acatgcacgt cgcccgctgc
      961 gctgcgcttc tcgcgcacga cccgctctac ctcttccgcc gacacgacca cgcgcatctc
     1021 cggatagagc ctccagagct cggcgaagat cttgaggatc acgcgcggtg aagaaaggtg
     1081 cgattcgccg cggatgcgcg ggaagtaatc gagtccgatc tgctggatcg agaagccgcg
     1141 gttcttcgct ttgacgatga gctccgcgtc gatcagcgat ccttccgact tcagctcgac
     1201 ggcatcgagc acctcgcgct tcatcagctt gaacgagaag ttgatgtcgc ggtgcggcca
     1261 gccgaagaga acgccgatca gaccgttgta gagatacgag tagagcgtcc gccttggccc
     1321 ttccagcgtc cggtcgagac gatatccggc gatgagatcc gctccggtca cagccatcgc
     1381 ccgcatggca cgtcccagcg cgtcgggatc gaagggtaag tctgcgtcca tgtagagcac
     1441 gagatctttc ttcgccgccg cgaatccggt cttgagcgac ccgcccagct tgcggttgcg
     1501 ctcgtggcgg atcatgcgga tgcgcggatt ctcggcagcg cggcaggcca ccaggaacgg
     1561 cgactgatcc gtcgaagcgt cgtcgacgat gatgatctcg tagtcgtccg tgtatttggt
     1621 gcaggcctcc accgccgcag tcatcgcatg ctcaatgttc aacgcttcgt tgaacatcgg
     1681 aatgacgagc gagacggatg gcgtgcgcat gatggaattt ggacaggcaa tgataccgaa
     1741 caacgacggc ttcgtcttca ttgccgtccg gttaatggct cgactggtca ggcggacgcg
     1801 ctgactggtg gcgcggatga gttgagtggt ggcgcgcacc agttaactgg tgacgctgac
     1861 cagttgactg gtggcgccca ccagttaact cgtggcgccc acgagacgac tcgtggcgct
     1921 gacgagttga ctcgtgacgc ccacgagttg actccgtggc gctgacgaga gcactcgtga
     1981 cgcccacgag ttgactcgtg acgctgacga gttgactcgt gacgctgacg agagcactcg
     2041 tgacgcccgc gagttgactc cgtggcgctg acgagaacac tcgtgacgcc caggagttga
     2101 ctccgtggcg ttgacgagag cactcgtgac gcccacgaat tcatccgtta cgcccacgga
     2161 atgactcgtg gcgctcacga gtcgagtgtt ggcgcgtagc attcgcggga tggcacggac
     2221 cggttcatcg gtgccacgca cgctctcgtg tgtcgcggtt acgggatcgg ccatgcgcgg
     2281 aatgagtgcg tccttcgcct cttcgcccac gaacgtcacc gtcatggccc acgcagccct
     2341 cgccgtagcc gctaggattg gtctgaggca aaggagtgtg ggcgtctcgc ccgcggccat
     2401 agggcgtctc gcccgatggt ggttagccag atgtcgcgcc agacaagtaa cgcaccggac
     2461 ccgtcgcgag atcaccgccg gacgagacgt ccgccggccg caggcgagac gcctgcactc
     2521 ctctgggtag ggaaaaccgc gcattcgtcc ctccttatgt gcagcacaag gagaacgcga
     2581 tgagattcgg tgtctgttgg caaccccgcg tgtggcacgg atggctacca ccgattccga
     2641 cgcttgcatc atggcaggcg gttcaggcag ccggcatcac ggacctgcgc ctgacctacg
     2701 atccggcgct cccgaccggc ctgttcgaca ccgatctgcg caatgcacat gcagctggct
     2761 tgcgcatcaa cgtgaacatg ggtgcggtgt gggagtccgg cggcgcgccg atcgacccgg
     2821 atccggcaat catcgaagcg caggcattcg atctcgccac caggttcggc gacatgatcg
     2881 cgctttacag tttcgacaac gagcccggcg ccatcgcaca aacgtacgag gccgcgaatc
     2941 cgggatcgga cttcatgcag gacgcctaca tgcctgcatg cactgcgttt gtgcgcggca
     3001 tccgccgcgt agctccggac gccatcatcg gcgggtgcga cgctgacagt gtcgacattc
     3061 agagccggta catcgaactt gcggacgcac tgctgccggt ggtcgtctgc gaccggcgac
     3121 gggttcatcc ctatggcgac gtcggcggtg cgcggtacgc cacgatggca ggcgatgatg
     3181 agaacgacgg cttcgcacag gcaacggacc cgcggccgct cgacgtcggt gagatcgatc
     3241 accagcaact cccgggagca accggcatcg ctcggccgag cgaccttcag gcactgctcg
     3301 ccttcacacg gatgatccat gcgaagtatc cgaccgtcgg tcagattgac tatggaacgg
     3361 ccgagtattt cttcacccgg ctgccagcgt ctgggatcgt tccggcaacg acatggagca
     3421 cctggacgtt cggaccggaa ccggtagtgt cggacgccgg ccgccagttc acgcagttgt
     3481 ttcatacgat caacgcggca cagccatcgg cagatgcgac cgcgatcgcg ccaccggttt
     3541 tcggcggcga tccgatcgtc attcagtgaa gttgagtacc cgtctccacg tgcagcagtt
     3601 gcaccgccag tgtgcgggga tcctggttgt gcatcagagt gctcggctgg aacgaccgcg
     3661 atgagcgcaa tcgcacgtcg atacgtggat cggatggcag ctccagattc gcgaccaccg
     3721 cctcccttcc gacgtgaagg accgcggaat cacctccatt gatcgagatc gtcacgtcat
     3781 cagcggtgta aggcagatct cccggtatcc gcagcgtgag cgacgcggtg gcgccgtgcg
     3841 attgcggcag acgaatggtc gcatcggggc cgagccagcg ccattccgtc ccctccagcg
     3901 tccgctcgat cggatacgcg ccgctcaacg ggagataacg ttcggcggcg cgcacattct
     3961 cgatcgtgac ttcctggtac aaaccacgcg tgagcttccg gtacgcgtca ctctccggcc
     4021 aggcgaagac atgcgagtcg gcgccatgcg ctccgccatt cgctaaaagc accagtcctg
     4081 ttcccggccg gtcatacgcc tcggcgatcg ctttatcgac cggcagcagc cggaagcgcg
     4141 acatcagata ctccgcatgc ggcttgagcg cgatgtcgta accgagcacg ctgttcggag
     4201 cgaggttcgc actcgcccag gccgctgctg cagcaggcgg tgatgccagc ttcgtccgcg
     4261 ttccggcgag cggccagaca aacaccagcg tcgccgcagc aaggatcgcg gtcactgccc
     4321 acggagccgc ggcaaagccg gcgctgcggc ggatcacgcc gaatccgagc gcggcgagca
     4381 gcgcgatgaa cggcatgaac gcgaggctgt agcgcgctcc gtccgccgga tccatccagc
     4441 cgagctcgaa ggtcacctgc acgatgacga aggcgatgaa cggcagaagc gttgagcgca
     4501 ccttgtcgcg gcgcagaagc ctggcgaaag cggcgacgcc gagcgtgacg caggcgaaga
     4561 gagggagcgt cacgtacttc cagccccacg gatgaaagat gaaacgcacc accagcatcg
     4621 ccgtcgacat cccgccgcgc gacaccgagg cgtcgtgcgc ggcgacgtat tgcatctgct
     4681 tcgtctcgta gaggcgcaga gcgttgaagc c
//
LOCUS       DATLVW010000925         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_535908, whole genome shotgun sequence.
ACCESSION   DATLVW010000925 DATLVW010000000
VERSION     DATLVW010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535908"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VNN25_27430"
     CDS             complement(<1..1918)
                     /locus_tag="VNN25_27430"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXH95338.1"
                     /translation="MLKIVLRTVIAAAFIVTPLVAQPGVAAKKAPTHEAIWMMKRVGT
                     PVPSPDGRWVVYPVTEPSYDEKEVSSDLWMIRPDASAPARIITNTKSPESDVTWSPDS
                     QRIAFAAKREGDDATQIYILDIGGGEAQRVTSISTGARSPQFSPGGTAILFTSVVYPG
                     ALDDDANRKIAKERKEQKYKVRTYQSFPIRSWDRWLDDMQVHILVQKLEPGAKPHDLL
                     APTQLVKQPGFAGRITEGSRDELDATWSPDGKSIVFVATTARNTSAYAEYGTDLYRIS
                     AVAGENAEPQVIAHAEGSYVRPRFSPDGKSLFAVFNANNGKVYNLDRLIRYDWPSMQN
                     RVVVTAPPFDRSVGSFAVAYDSKSVYFTAEDAGLEKIYSVGAGGGNVSLTVDPPRGVY
                     TSLTSAEHAPLLIAKWGSSVEPPEVVRVDLATKSHRNLTEINVANARQLDWQSPRHFW
                     FASKRGKKIHNMIVLPPGFDETKKYPLFVVIHGGAANMWRDEISLRWNYHLLAKPGYV
                     VLLTNYTGSTGFGEKFGQDIQGDPLKGPGEELNEAADEAVKRFAFIDGTRMAAGGASY
                     GGHLANWLEATTTRYKCLVSHAGEINLESQWGTSDGIYHRELTNLGPVWEQGKVWREQ
                     NPIRYAAKFHTPMLL"
BASE COUNT          358 a          660 c          693 g          457 t
ORIGIN      
        1 agaggagcat cggcgtgtgg aactttgccg cgtagcggat cgggttctgc tcgcgccaca
       61 ctttgccttg ctcccacacg ggcccgaggt tcgtgagctc gcgatgatag ataccgtcgc
      121 ttgtgcccca ttgcgactcc aggttgatct cgccggcgtg gctgacgagg cacttgtagc
      181 gagtggtcgt cgcttccagc cagttcgcga ggtggccgcc gtaactcgcg ccgcccgcgg
      241 ccatgcgtgt gccgtcgatg aacgcgaaac gcttgaccgc ctcgtccgcc gcttcgttga
      301 gctcctcgcc gggccctttc agcggatcgc cctgaatgtc ctgcccgaac ttctcgccga
      361 agcccgtcga tcccgtgtag ttcgtcagca ggacgacgta tcccggtttc gccagcaagt
      421 gatagttcca gcgcagcgag atctcgtcgc gccacatgtt ggcggcgccg ccatggataa
      481 cgacgaagag cgggtacttt ttcgtttcgt cgaagccggg tggcagcacg atcatgttgt
      541 ggatcttctt cccgcgcttg ctggcgaacc agaaatgccg cggtgactgc caatcgagtt
      601 ggcgcgcgtt cgcgacgttg atctccgtca agttgcggtg actcttcgtc gccagatcga
      661 cccgcacgac ctcgggtggc tcgaccgaac ttccccactt cgcgatgagc aacggcgcgt
      721 gctccgcgct ggtcaggctc gtgtagacgc cccgaggcgg atcgacagtg agcgaaacgt
      781 tgccgccacc cgcgccgacg ctgtaaatct tttccagccc cgcatcttcg gcggtgaagt
      841 agacggactt cgagtcgtag gccacggcaa acgagccgac cgagcggtcg aatggcggcg
      901 ccgtcacgac gacgcggttc tgcatcgacg gccagtcgta gcgaatcagg cgatcgaggt
      961 tgtagacctt gccgttgttg gcgttgaaga ctgcgaagag cgacttccca tcaggactga
     1021 atcgcggccg tacgtacgat ccctcggcat gcgcgatgac ctgcggctca gcgttctctc
     1081 cggcgaccgc ggagatgcga tacaaatcgg tgccgtactc tgcgtatgcc gaggtgttgc
     1141 gcgcggtcgt ggccacgaag acgatggact ttccatccgg cgaccacgtg gcgtcgagct
     1201 cgtcgcgcga gccttccgtg atccgccctg cgaagcccgg ctgtttcacg agctgcgtcg
     1261 gcgccaggag atcgtgcggt ttcgctcctg gctccagttt ctgaacgagg atgtgaacct
     1321 gcatgtcatc gagccatcga tcccaactgc ggatcgggaa cgactggtac gtgcgcactt
     1381 tgtacttttg ctctttgcgc tctttcgcga tcttgcgatt ggcatcgtca tcgagcgctc
     1441 ccggatagac gacgctggtg aaaaggatgg ccgttccgcc cgggctgaac tgcggcgagc
     1501 gcgcgccggt ggagatcgac gtgacgcgct gcgcctcgcc gccgccaatg tcgaggatgt
     1561 agatctgcgt cgcgtcgtcg ccttcgcgtt tggcagcgaa ggcgatgcgc tggctgtccg
     1621 gcgaccaggt gacgtcgctt tccggagact tcgtgttcgt gatgatgcgc gccggcgcgc
     1681 tggcgtcggg acggatcatc cagagatcgg acgacacttc cttctcgtcg tacgagggct
     1741 cggtcaccgg atagacgacc catctgccat cgggactcgg caccggcgtc ccgacgcgtt
     1801 tcatcatcca gatcgcctcg tgcgtcggcg ctttcttcgc ggcgacaccg ggttgcgcga
     1861 caagaggcgt gacgatgaac gcggcggcaa tgaccgttcg caggacgatt ttcaacatgc
     1921 gtgatctcct cagtgatgag tgtgatgcgg agagtatcgc agggatttgg cgcgttcgcg
     1981 tcatgcgccg cctctcgaca gatgaaagag tggggttggg gaggcttggc cgacctcgcc
     2041 tgaattgggc tctctacatg gatgcgagag actcaatcga gccgccatgg gcaagctgga
     2101 agagctcggt cgtatgccgt cgtttagcga tttttcggcg cggttaacag ctttcatgtc
     2161 agctactt
//
LOCUS       DATLVW010000926         3669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_616989, whole genome shotgun sequence.
ACCESSION   DATLVW010000926 DATLVW010000000
VERSION     DATLVW010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616989"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..694
                     /gene="rimP"
                     /locus_tag="VNN25_27435"
     CDS             227..694
                     /gene="rimP"
                     /locus_tag="VNN25_27435"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXH95339.1"
                     /translation="MSGLPERAAQEIETIVSSEGLELVHIEYRKQGHGFLLRIDIDKE
                     GGVTVEDCELVSHQVSAFLDVDDVVPAAYELQVSSPGLDRKFYKLSDYEKFIGRLVRV
                     RTSKAVRGLHVIVGRLKEFNGETVVVTDPVMKKDPDYVVPLADIKETRLEVEI"
     gene            805..2355
                     /gene="nusA"
                     /locus_tag="VNN25_27440"
     CDS             805..2355
                     /gene="nusA"
                     /locus_tag="VNN25_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXH95340.1"
                     /translation="MFDENLSKNIKALAYEKSIDVERVFKALEDALATAARKYYKTRE
                     PMETVIDRVSGAMTMYVIKDVVIDENEIEDPQAQWTLEQARKIDPEIGVGGRIRLSYI
                     TKEVVDVVKDSNTQIRTYEAQKIDSEVEQGDEVHIPLTKAAEDFGRIAAQSAKQVLYQ
                     KVREAEREKVYNEFIGKAGELENGYVKRFERGDMIIDLNGKTEGIIPRSQQSRAERYS
                     QGDRIKAVIAEVHTQPKGPQVVLSRTDPRLLIKLFEMEVPEIYDGTVVIKGAVREPGE
                     RAKIAVTSRERDVDPVGACVGMKGSRVQSIIRELRGEKIDIIPWNEDIVIYAQNSLAP
                     AKITRVSVTSDEQAHRPHLDVIVDNDQLSLAIGKRGLNVRLAAELIGAKIDIKSEEEV
                     KGEVADALSAMLQEAMAESRAETNVHDIEEMPEDWANTLEAAGYDDLDSVINASVEDL
                     IAIEGVDEEMAQQMIEIARKHEQIDETEPAGDEEEEEYQASEGDEGGEEAAEPENAET
                     PQEEAPVE"
     gene            2463..>3669
                     /locus_tag="VNN25_27445"
     CDS             2463..>3669
                     /locus_tag="VNN25_27445"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HXH95341.1"
                     /translation="MAAAKFRVSDIAKKMGKSEKDVIFQLQSLGAEITDASQVLEPEV
                     VQALITGKKLTMRTRNVIMREDKPQVAAKKEAVRPPRAIVKPTPRAKREVPVEEPQVV
                     APPPTVIIPEFVEAAEAEERAAKEAPPVAAKAPRPAAPPPPPPPPAPEPAAPPMPMAA
                     APAAATPAPTPAPAHPAPPPAAGPGLRSGPTGPRARAPQATRPNYAGQQPVARPSYPP
                     SPARPSGPGAPRPGGPMGTRPTGPAGSRPPMGTRPSGPGGPRPGGPMGARPSGPGSFR
                     PGVGRPGGGVTPPPPPPGTLPGGAMGRSKARRPDGGEDLSPKKKTTGKRGKSERPAEV
                     DNELFTKGPFSGTQIINDDLGPDIPILDTDEDGKPRVPSNRRDVRKQQAPVSGKVLNF
                     NRPTNKIPLS"
BASE COUNT          806 a         1121 c         1155 g          587 t
ORIGIN      
        1 gacgatccat cgtgcggcga agcatcagtt gtaagagcaa tttagactcg gtttgaatcg
       61 acggccgcgc tttcgggcgc ggccgttttt ctgtactgcc gcagcggaat cgcgccgttg
      121 ttcacgatgt tgtgacggct cgctgcaaag tggtatcatt cgtcagcttt cgagcttcaa
      181 cctatcgaaa gcggccgatc ggccgctttt tttgttgtca ggagatatga gcggtttacc
      241 cgaacgggct gcacaggaga tcgagaccat cgtttcgtcg gaaggtctgg agctcgttca
      301 cattgagtac cggaagcagg gtcatggatt cctgctgcgg atcgacatcg acaaggaggg
      361 aggcgtcacg gtcgaggatt gcgagctcgt ctcgcaccag gtcagcgcgt ttctcgacgt
      421 cgatgatgtc gtccccgcag cgtacgagct gcaggtgtcg tctcccggtc tcgaccggaa
      481 gttttacaag ctgtcggatt acgagaagtt catcggccgc cttgtccgcg tcaggacgtc
      541 gaaggcggtg cgggggcttc atgtgatcgt cggaagactg aaagaattca acggcgagac
      601 ggtcgtcgtc acggatccgg tgatgaaaaa ggatcctgac tacgtcgtcc cgctcgcaga
      661 catcaaagaa acgcggttgg aagtggaaat ctaggtaacg cagttgcgaa gtacgtaggt
      721 cggactctcc agtccgacta gacgagaagt cggactggag agtccggcct acgtaccccg
      781 caacttcgct actccgggag cagaatgttc gacgagaacc tcagtaaaaa catcaaggcg
      841 ctggcgtacg agaagtcgat cgacgtcgag cgtgtgttca aggcgctcga ggatgccctc
      901 gctaccgctg ccaggaagta ctacaagacc cgcgagccga tggaaaccgt catcgaccgc
      961 gtgagcggcg ccatgacgat gtacgtcatc aaagacgtgg tcatcgatga gaacgagatc
     1021 gaagatccgc aagcgcagtg gacgctggaa caagcccgga agattgatcc cgagatcggc
     1081 gtcggcggcc gcatccggct cagctacatc accaaggaag tggtcgacgt cgtcaaagac
     1141 tcgaacacgc agatccgcac gtacgaagcg cagaagatcg actccgaggt ggagcagggc
     1201 gacgaagtcc acatcccgct gacgaaggcc gccgaagact tcggccgcat cgccgcgcag
     1261 tcggccaagc aggtgctcta ccagaaggtg cgcgaagcgg agcgcgagaa ggtctacaac
     1321 gaattcatcg gcaaggcggg cgagcttgag aacggttacg tcaagcgctt cgagcgcggc
     1381 gacatgatca tcgatctcaa cggcaagacc gagggcatca tcccgcgctc gcagcagtcg
     1441 cgcgcggagc gctactcgca gggcgaccgc atcaaggcgg tcatcgccga agtccacacg
     1501 cagccgaaag gtccgcaggt ggttctctcg cgcaccgatc cgcgtctgct catcaagctc
     1561 ttcgaaatgg aagtgccgga gatctacgac ggcacggtcg tcatcaaagg cgccgtccgc
     1621 gagccgggcg agcgcgccaa gatcgcggtc acgtcgcgcg agcgcgacgt cgatccggtc
     1681 ggcgcatgcg tgggcatgaa aggctcgcgc gtgcaatcga tcatccgcga gttacgcggc
     1741 gagaagatcg acatcattcc ctggaacgaa gacatcgtca tctacgcgca gaactcgctg
     1801 gcaccggcca agatcacgcg cgtttcggtg acgagcgacg agcaggccca ccgtccgcac
     1861 ctcgacgtca tcgtcgacaa cgatcagctt tcgctcgcca tcggcaagcg cgggctgaac
     1921 gttcgtctcg ccgcggagct gatcggcgcg aagatcgaca tcaagtcgga agaggaagtc
     1981 aaaggcgagg tcgccgacgc cctcagcgcc atgctgcagg aagcaatggc cgagtcgcgc
     2041 gcggaaacga atgttcacga catcgaagag atgccggaag attgggcgaa cacgctcgaa
     2101 gcagccggtt acgacgatct cgattccgtg atcaacgcct cggtcgaaga tctcatcgcg
     2161 atcgaaggcg tcgacgagga aatggcccag cagatgatcg agattgcccg caagcacgag
     2221 cagatcgatg agactgagcc tgccggcgac gaagaggaag aagagtacca ggcatccgaa
     2281 ggggacgagg gcggcgaaga agccgctgaa ccggaaaatg ccgagacacc gcaggaagag
     2341 gcgcctgtgg agtagaagaa agttgttagg ttgcagggtt gctcagttgt tgagcgagtc
     2401 ggcggctgaa ccagcgcaac cacggcaact cggtaaccag tagtcaggaa ccaggagatt
     2461 caatggcggc agcaaaattt cgagtgagcg acatcgcaaa gaagatgggc aagtccgaga
     2521 aggacgtcat cttccagttg cagtcgctcg gcgctgagat cactgacgca agtcaggtcc
     2581 tcgagccaga ggtcgttcag gcgctcatca ccggcaagaa actgacgatg cgtacgcgca
     2641 acgtcatcat gcgcgaagac aagccgcagg tcgctgcgaa gaaagaagct gtccgcccac
     2701 cgcgtgccat cgtgaaaccg actccgcgtg cgaagcgcga ggtgccggtg gaagagccgc
     2761 aggttgtcgc tccgccgccc acggtgatca tccccgagtt cgtcgaggct gctgaagccg
     2821 aagagcgtgc tgcgaaagaa gctccgcccg tcgctgcgaa agcgccacgt cccgcagcac
     2881 cgccgccgcc accgccgccg cctgctcccg agccggccgc accgccaatg ccgatggcag
     2941 ccgcaccagc ggccgcgacg cctgcaccaa cgccagcgcc ggctcatccg gcgccgcctc
     3001 ccgccgccgg acctggactg cgctccggac cgactggtcc ccgcgcccgg gcaccgcaag
     3061 caacgcgtcc gaattacgcc ggtcagcagc cggtcgcccg tccaagctat ccaccaagcc
     3121 ctgcacgtcc ctcaggtccc ggcgccccgc gtccgggtgg tccaatggga acgcgtccaa
     3181 ccggtcctgc cggttcacga ccgccgatgg gaacacgtcc gtccggccct ggcggtccgc
     3241 gtcccggtgg tccgatgggc gctcgtccct ccggtcctgg aagctttcgt ccgggcgtcg
     3301 ggcgtcccgg cggcggcgtc actcctccgc cgccacctcc cggcacgttg ccgggtggag
     3361 cgatgggtcg cagcaaagcg cgtcgtcctg acggcggcga agatctttcg ccgaagaaga
     3421 agacgaccgg taaacgcggc aagagcgagc gcccggcaga agtcgataac gagctcttta
     3481 cgaagggtcc tttctcagga acccagatca tcaacgatga cctcgggccg gacatcccca
     3541 tcctcgacac tgatgaggat ggaaagccgc gcgttccgtc caatcgccgc gacgttcgca
     3601 aacagcaggc tccggtctcg ggcaaggtcc tgaacttcaa tcgtccgacg aacaagattc
     3661 cgctcagcg
//
LOCUS       DATLVW010000927         7005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_409648, whole genome shotgun sequence.
ACCESSION   DATLVW010000927 DATLVW010000000
VERSION     DATLVW010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409648"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VNN25_27450"
     CDS             <1..728
                     /locus_tag="VNN25_27450"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXH95342.1"
                     /translation="KFIVLDRVNPIGGAAFEGPLLHGEESFIAWHSLPIRHGMTVGEL
                     ARMFNDERHIGADLTVIPLQRWRRELWMDEAGLPWINTSPNMRSLTEATLYPGIGILE
                     QAVSVGRGTDTPFEILGAPYVDGATLARELTAMSLPGIRFEATEFTPASSNFAKQRCG
                     GVRMTITDRNAWRPVATGAAIALVLHRLYPNDFALDKLANLLRDPATLEAIRANKPLS
                     EITAMWRDDEAAFAVRRAKDLLY"
     gene            complement(812..2143)
                     /locus_tag="VNN25_27455"
     CDS             complement(812..2143)
                     /locus_tag="VNN25_27455"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXH95343.1"
                     /translation="MVTAVVTMLMTIGAAAQNNAAQHDHSMMAMSAEAEAEHLARLKA
                     VLEAGSSHGPVIPQPENVQGLAVQNIAMTAKSFSFTPNSITVNQGDLVNITFSVPSND
                     PSSVGHGLLMETYVENGVNVARGSSKLISFTATTAGNFQFACNVPDCGTGHSSMFGLL
                     IVKAVANPAPTISSIAPTSGVTPGGTQVTITGTGFLSGATVKFDGIAAFIVSVTATSI
                     VAVIPPHAAGKVDVVVANTDGQSATLPQGFTYALPAPTITDVSPATGPTTGGTLITIT
                     GTNFQSGATVKIGASQATDVNVVSSTSITARTPLGPVTAQLAVDVVVTNPDSTKATAT
                     GGFTYSVPPLAVISVTPNIVLPLGAPGAGPVVVTIFGAGFTTTLPTTVTVDGVAATGV
                     QVVDPVTLHATFPARAAGTKDVVVTVGSTSATLKNGFSWQNAPSRHRASKP"
     gene            complement(2234..3430)
                     /locus_tag="VNN25_27460"
     CDS             complement(2234..3430)
                     /locus_tag="VNN25_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HXH95344.1"
                     /translation="MSTIAPEPAPSERLLALDVFRGITIAGMLLVNNPGSWSAIYPPL
                     EHADWNGWTPTDLVFPFFLFIVGVTTHLSLSKRDPATLTMKIIRRGLMIVLAGVLLNA
                     FPFFWWGKMAGIPDPTFLQRVLWRAQHLRYMGVLQRIGFAYIGAALLTLRTSWRQQNA
                     IVVAILLGYWMIMTLVPVPGTGTIGYFLLDQPDKTLAAYTDRLILGTNHIWVSTKTWD
                     PEGPLSTLPAIATAMLGIFAGRWLTDKKRSLLERIAGLFGVGCLAMVAGELWSWVFAI
                     NKNLWTSSYVVFTAGFACVMLATCLWILDVNQVRGWSRPFAIYGLNPLVAFVGSGVMA
                     RCTDSLIKLDVGGKKVSLHQAVYDVVFAPWFPPKFASLLGGLAFVAFWLAILWLLERR
                     NIIVKL"
     gene            3505..4902
                     /locus_tag="VNN25_27465"
     CDS             3505..4902
                     /locus_tag="VNN25_27465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95345.1"
                     /translation="MRRTTRALLFLFCVVMLCIGATRRRTATPPQSAPLDARRSFAVT
                     DKAILDGFSFERVLTTLTAGTQTTPVQLYQQWLDTFNAKPGLVSPDGPHCDDFLVDGG
                     ASFNGFPRRCPVPEGLLANDPPFTPGRFIPIGIINRFDLAPADGSNCGQYRLIFARPS
                     PVVSDLFHIIFEAVLPNPHPEAGLYGCRPVAAFWADLSAVGSMDERRAQLEMFFFQGL
                     PGFSPVVRAENYSGAGGIRTSHNRTGATGLRMYQFRATRDCTAADCRITMRPDVLENM
                     IFGTFFDSHLDTPQARRFRDNFVTQVSTLAVRDVNEYFMNVPSEYLMAESNPSDTVPL
                     FILDSPFTIGLTTSEGTDFNNRIAAELTRIGSTLTPSQIVQRADTQTCIGCHVVSGPV
                     GEGVVFPRPGDHLQQIDEHLLEPGEAGARFTISAAMQVFIPHRMKVMQDFLNSGKAPV
                     HSDSGATIGGGRSVQ"
     gene            complement(4960..5787)
                     /locus_tag="VNN25_27470"
     CDS             complement(4960..5787)
                     /locus_tag="VNN25_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH95346.1"
                     /translation="MSYSVREVSAMLGLSAAQIRSYAAKGFLTPERDAGGDLHFGFQD
                     LVILRTAAELTAAHIPTRKIRRVLASVREQLPEGRSIAGVRIAADGERVVVRDGETVW
                     NPESGQSLFDFSVAEIADKTKPVALAAVRDARSRREDDLGADAWYELGSDLEVSAPEE
                     ARDAYEKAVALDPAHIDAHVNLGRLLHENGEPAAAEVHYRAALKADAQHPVAMFNLGV
                     ALDDLGRLTEAADTYRRALELDPENPDAHYNLAGIMERQGDKAGAVRHLTRYRALTR"
     gene            complement(5879..6775)
                     /locus_tag="VNN25_27475"
     CDS             complement(5879..6775)
                     /locus_tag="VNN25_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXH95347.1"
                     /translation="MPRAFASGQIAFGLVSIPVKLFSATEASEKISFNMIHKDCGNRV
                     QQQLFCPKDERTISREEVVKGFEFAKGQYVLFNEEELKTLEEKATQAIEISEFLPKEA
                     IDPIYFAKANYVAPDKGGERAYSLLTKAMEQTGRWALAKYAARGKQYLVILRPLGNGL
                     VMQQLFYPNEIRSMDELDLGDAIVKDNELKMAVQLAEMGAADEFHPENYRDEVADRIR
                     ATIQRKIEGEEITSNLVEEPRAQVIDLMEALKASLAKPRAKSSTKSNAAPVRMQAKAE
                     EKRKPAQASARKVAAKAGSRSR"
     gene            6876..>7005
                     /locus_tag="VNN25_27480"
     CDS             6876..>7005
                     /locus_tag="VNN25_27480"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXH95348.1"
                     /translation="MPLSDIRPPKPDTRRKAIYAAVKRIPRGRVATYGQIATIAGLD"
BASE COUNT         1283 a         2190 c         2173 g         1359 t
ORIGIN      
        1 tcaagttcat cgttctcgac cgcgtcaatc ccatcggcgg cgccgccttc gaaggaccgc
       61 tgctgcacgg cgaagagagt ttcatcgcct ggcattcact ccccattcgc cacggaatga
      121 ccgtgggcga gctggcgcgg atgttcaacg acgagcggca catcggcgcc gacttgaccg
      181 tgattccgct gcagcgttgg cggcgcgagc tctggatgga cgaggcaggc ctgccgtgga
      241 tcaacacctc gccgaacatg cgcagcctga ccgaagcgac gctctatccc gggatcggca
      301 tcctggagca ggctgtctcg gtcggacgcg gcaccgacac tcccttcgag atcctcggcg
      361 cgccatacgt ggatggcgca acgctcgcac gcgagctgac ggcaatgtcg cttcccggca
      421 tccgcttcga agcgacggag ttcacacctg cgtcaagcaa cttcgcgaag cagcgctgcg
      481 gcggcgtgcg gatgacgatc accgatcgca atgcatggcg gccggtcgca accggcgccg
      541 ccatcgcgct cgtgctgcac cggctctacc cgaacgattt cgcgctcgac aagctggcta
      601 acctgttgcg cgatccggcc acgctggagg cgattcgggc aaacaagccg ttgtccgaga
      661 tcaccgcgat gtggcgcgac gatgaagcgg cgttcgcggt acggcgggcg aaggatttgt
      721 tgtattgatt cgaaaagcat tcaaccacag agacacagag gacacagaga gctctgtgct
      781 ctctgtgcct ccgtggtgat gttctttctg ctcagggttt cgaggcgcga tgtcttgacg
      841 gcgcgttctg ccaggagaat ccgttcttca gcgttgcgct cgtgctgccc accgtaacca
      901 cgacgtcttt cgtcccggcg gctcgcgccg gaaacgtcgc atgcaacgtg accggatcga
      961 ccacctgcac accggtcgcc gcgacgccgt cgacggtcac agtcgtcggg agagtcgtgg
     1021 tgaaacccgc gccgaagatc gtgacgacta ccggtccggc gccaggcgcg ccaaggggga
     1081 ggacaatgtt cggggtcacg ctgatcaccg ccagcggcgg caccgagtag gtgaacccac
     1141 ctgtcgctgt cgctttcgtg ctgtcaggat tcgtgacgac gacatcaaca gcgagttgtg
     1201 ccgtcacggg accaagtggt gtccgcgcag tgatcgatgt cgagctgacc acattgacgt
     1261 ctgtcgcctg cgacgcgccg atcttcaccg tcgcgccgct ctggaagttc gtccccgtga
     1321 tcgtgattag cgtccctccg gttgttggtc cggtggccgg agaaacatcg gtgatcgttg
     1381 gcgcgggcaa agcgtaggtg aagccctgcg gcagcgtcgc cgactgtccg tcggtgttcg
     1441 cgaccacgac gtcgactttc cctgctgcat gagggggaat cacggccacg atcgaagtag
     1501 cagtcaccga tacgatgaat gccgcgattc cgtcgaattt gaccgtcgct ccgctcagaa
     1561 atcccgtccc ggtgatcgtg acctgcgtcc cgccgggcgt gaccccgctc gtgggagcga
     1621 tgctgctgat cgtcggtgcc ggattggcca ccgctttcac gatcagaagg ccgaacatgc
     1681 tggagtggcc ggtgccgcag tccgggacat tgcacgcaaa ctggaagttg ccggccgtcg
     1741 tggccgtgaa cgagatcagc ttggaagagc cgcgcgcaac gttcactccg ttctcgacgt
     1801 acgtctccat caataaaccg tgacccacgc tcgaagggtc gttggagggg acgctgaacg
     1861 taatgttcac cagatccccc tggttcaccg tgattgagtt gggcgtgaac gaaaagctct
     1921 tcgccgtcat ggcgatgttc tggaccgcga ggccctgaac gttctccggt tgcggaatca
     1981 ctggtccgtg cgacgagccc gcttcgagga cagccttcag ccgcgcaaga tgctccgcct
     2041 ccgcctcggc agacatcgcc atcatcgaat gatcgtgttg tgcagcgtta ttttgtgcag
     2101 ccgcgccgat cgtcatgagc atcgtgacaa cggctgtaac catcgctctc cgcatcaagg
     2161 tctcccgaaa catgagtttt tcaagtccaa gcttgaagaa tacccacatt cgacagccgc
     2221 gcaaccgcgc tgtttacaac ttcacaatga tgttgcgccg ttcgagcagc cagagaatgg
     2281 ccagccagaa cgcgacgaag gcgagtccgc cgaggaggga agcgaacttt ggggggaacc
     2341 agggagcgaa gacgacgtcg tagacggcct gatgaagcga gactttcttc ccgccaacgt
     2401 cgagcttgat gagcgagtcg gtgcagcgcg ccattacccc cgagccgacg aacgccacga
     2461 gaggattgag gccgtagatt gcgaacggcc gcgaccagcc gcgaacctgg ttcacgtcga
     2521 ggatccagag gcaggtggcc agcattacgc aggcgaagcc ggccgtgaag acgacatagc
     2581 tcgacgtcca gagattcttg ttgatcgcaa agacccaact ccacaactcg ccggcgacca
     2641 tggccagaca tcccacgccg aaaagccctg cgatccgctc gagcagcgag cgtttcttgt
     2701 ccgtcagcca acggccggcg aagatgccga gcatggcggt ggcgatcgcc ggcagcgtcg
     2761 agagcggacc ttcgggatcc cacgttttcg tcgagaccca gatgtgattc gtgccgagga
     2821 tgagacggtc ggtgtaggcg gccagagtct tatccggctg gtcgagaagg aagtagccga
     2881 tcgtgcccgt gccgggaacg ggaacgagcg tcatgatcat ccagtagccg aggaggatgg
     2941 ccacgacgat ggcgttctgc tgccgccacg aggtgcgcaa cgtcaggagc gccgcgccga
     3001 tgtacgcaaa tccgatccgc tgcaggacgc ccatgtagcg caggtgctgc gcgcgccaca
     3061 gaacccgctg aagaaatgtc gggtcaggga tgcccgccat cttcccccac cagaagaagg
     3121 ggaaggcgtt gagcagcacg ccggccagca cgatcatcag gccgcgacgg atgatcttca
     3181 tcgtcagcgt ggccgggtcg cgcttgctga gcgagagatg cgtcgtcacg ccgacgatga
     3241 agaggaagaa cgggaagacg aggtcagtcg gcgtccagcc gttccaatcc gcatgctcga
     3301 gcggcggata gatcgcgctc caactccccg gattgttgac gagcagcatt ccggcgatgg
     3361 tgatgccgcg aaagacgtcg agcgccagga gccgctcgga tggcgcaggc tcgggagcga
     3421 tggtggacac gtggtaaggg tagcaggtgt gactgtaacg accgcgaatt ccctgacgga
     3481 ggtgaacgcg ctaccatggg gattatgcgt agaacgactc gggcgctgct cttccttttc
     3541 tgcgtcgtga tgttgtgcat cggcgcgacg cggcgccgga ccgctactcc cccccaatca
     3601 gccccgctcg atgcccgccg gtcattcgct gtgacggaca aggccatcct cgatgggttt
     3661 tccttcgagc gggttctgac gacgcttacg gcaggaacac aaactacgcc tgttcagctc
     3721 taccagcaat ggctcgatac gtttaacgca aagcccgggc tggtctcgcc cgatggacct
     3781 cattgcgacg atttcctggt cgacggcggc gcttccttca acgggtttcc gcgccgctgc
     3841 ccggttccgg aagggctgtt agcgaacgat ccgcccttca cgccgggacg tttcatcccc
     3901 atcggcatca tcaatcgctt cgatctggct cctgccgacg gctcgaactg cgggcagtat
     3961 cgcctcatct ttgcccggcc ttcgcccgtc gtgtcggatc tatttcacat catcttcgag
     4021 gctgttcttc caaatccgca tcccgaagcc ggtctgtacg ggtgccgtcc cgtggcggca
     4081 ttctgggccg acttgtccgc cgtcggatcg atggacgaac gtcgcgcgca gctggaaatg
     4141 ttcttctttc agggattgcc cggcttctcg cccgttgtcc gcgccgagaa ttacagtggc
     4201 gccggcggca tccgaacgtc gcataatagg acgggcgcga cggggctccg catgtaccag
     4261 ttccgcgcca cgagagattg cacggcggct gattgccgca tcaccatgag acccgacgtt
     4321 ctggagaaca tgatcttcgg tacgttcttt gattcccatc tcgacacgcc gcaagcccgg
     4381 cgtttccgcg acaacttcgt cacgcaggtc agcaccctcg ccgttcgcga tgtcaatgag
     4441 tacttcatga atgttccgtc cgaatatctc atggccgaat ccaatcccag cgacacggtg
     4501 ccgctattca tacttgactc gccgttcaca atagggttga ccacatctga gggaacggac
     4561 ttcaacaacc gaatcgctgc cgagctgacg cggatcggaa gcactctgac accttcgcag
     4621 atcgttcaac gcgcggatac acagacctgc atcggctgcc acgttgtgag cggtcctgtc
     4681 ggtgaaggag tggtgttccc tcggccgggc gaccatcttc agcaaatcga tgagcacctg
     4741 ctggagcctg gcgaggccgg cgcacgtttt accatctctg ccgccatgca ggtcttcatc
     4801 ccccaccgaa tgaaggtgat gcaagatttc ctcaacagtg gcaaggcgcc ggtgcactcg
     4861 gactctggtg cgaccatcgg cggcggccgt agcgtgcagt agtcagaggg aatctctgtg
     4921 atctctgtgc ctctgtggtg aagcttcttt ggcacctacc tagcgcgtca acgcccgata
     4981 tctggtcaga tggcgcaccg ctcccgcctt gtccccttgc ctctccatga ttccagccag
     5041 gttgtagtgt gcgtcggggt tttcaggatc gagctcgaga gctcttcggt acgtgtcggc
     5101 ggcctccgtc aggcggccga ggtcatcgag tgcgacgccg aggttgaaca tcgccacggg
     5161 atgctgcgcg tcggctttga gcgcggcgcg atagtgaact tcggctgcgg cgggctcgcc
     5221 gttttcgtgc agcaacctgc cgaggttcac gtgggcgtcg atgtgggcag gatcgagagc
     5281 caccgctttc tcatacgcat cccttgcttc ctcgggcgcg ctgacttcga gatcggatcc
     5341 gagctcgtac caggcgtcgg caccgaggtc gtcctcgcgg cgagacctcg catcgcgcac
     5401 ggcggcgagc gcgaccggct tggtcttgtc cgcaatctcc gcgacggaaa aatcgaagag
     5461 cgattggcca gattccggat tccagaccgt ctcgccgtcg cggacgacga cacgctcccc
     5521 gtccgcggcg atgcggactc cggcgatcga tcgtccttcc ggcaattgct cgcgcacgct
     5581 cgccagcacg cggcggattt tgcgcgtcgg gatgtgtgcc gcggtcagct cggcggcagt
     5641 gcgaagaatg acgaggtcct gaaatccgaa gtggagatcg ccacccgcgt cgcgttcggg
     5701 cgtcagaaat cccttcgcgg catacgagcg gatctgcgcc gcggagagcc cgagcatggc
     5761 cgagacttcc cgcacggagt aggacatagc tcgattctag cggagagaac gcgcgtgagc
     5821 ggccggttgg cggcgagacg ccgccaggcc agcctccgga cggcagcgtt ccgctgtcct
     5881 aacgcgagcg cgagccggct ttggcggcaa cctttcgcgc cgacgcctgc gccggtttgc
     5941 gtttctcttc cgctttcgcc tgcatccgca ccggcgcggc gttgctcttc gtgctggact
     6001 tcgcgcgcgg cttggccaga ctggccttga gcgcttccat cagatcgatg acctgcgcac
     6061 gcggctcttc gacgaggttc gacgtgatct cctccccctc gatcttccgc tgaatcgtgg
     6121 cccggatccg gtcggcgact tcatcgcggt agttctccgg atggaactcg tcagcggctc
     6181 ccatctcggc gagctgcacg gccatcttga gctcgttgtc tttgacgatg gcgtcgccga
     6241 ggtcgagctc gtccatagac cggatctcgt tgggatagaa aagttgctgc atcaccaggc
     6301 cattgcctag cgggcggagg atgacgaggt actgcttgcc gcgcgccgca tacttcgcca
     6361 gcgcccagcg gccggtctgc tccatcgcct tggtgagcag cgagtaggcg cgctcgccgc
     6421 ctttgtccgg cgcaacgtaa ttcgctttcg cgaagtagat aggatcgatc gcttccttcg
     6481 gcaggaactc gctgatctcg atcgcctgcg tggccttctc ttccagcgtc ttcagctctt
     6541 cctcgttgaa gaggacgtac tgtcccttcg cgaattcgaa ccctttgacg acctcttcgc
     6601 gcgagatggt gcgctcgtct ttgggacaga agagctgctg ctggacgcga ttcccgcagt
     6661 ccttgtgaat catgttgaag ctgatcttct cgctcgcctc ggtggccgag aagagcttca
     6721 ccgggatcga gacgaggccg aaagcaatct gaccggaagc aaacgcgcga ggcatatcga
     6781 ctcctttagg tgaccaaaag ggtgtgcatg ttagcagatg gggggaatcg tgtgtcggga
     6841 gtcgggggtc ggagcgacgg cgataaactc tcgcggtgcc tctctccgac atccgacccc
     6901 cgaaacccga cacccgccgc aaagccatct acgcagcggt gaaacgcatc ccgcgcggac
     6961 gcgtggcaac gtacggacag atcgctacga tcgctggtct cgatg
//
LOCUS       DATLVW010000928         6218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_492646, whole genome shotgun sequence.
ACCESSION   DATLVW010000928 DATLVW010000000
VERSION     DATLVW010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492646"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VNN25_27485"
     CDS             complement(<1..523)
                     /locus_tag="VNN25_27485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXH95349.1"
                     /translation="MAEYFFELLTEEIPAWMHEAAHAALLQQLTKLTHDLGEPAGDRN
                     PVIVNSTPRRIIFFLSRIPLRESDREEEVKGPPIKAAYDAEGKPTQALNGFLKKNNAA
                     IEDLITGGDYIRIRRQIKGRAADEILQQRIPQIVESLRWPKMMRWGKGEHSYIRPIHS
                     VVSVLDGAHLPITI"
     gene            complement(523..1407)
                     /gene="glyQ"
                     /locus_tag="VNN25_27490"
     CDS             complement(523..1407)
                     /gene="glyQ"
                     /locus_tag="VNN25_27490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXH95350.1"
                     /translation="MPRLSFQDLIFRLQTFWAEHGCMIETPLDVAVGAGTMHPATFLR
                     VLGPEPWNAGYVQPSRRPADGRYGDNPFRLGKHYQFQVVLKPSPLNVQDLYLDSLRAI
                     GLDLTRHDIRFEEDNWEAPTLGAWGVGWQILLDGMEITQFTYFQQAGGIDLSPISAEI
                     TYGLERLTMFLSRQSSVYDIEWGAGFGYGPVRKQDEYEFSKYYFETADIDMHWQLFKL
                     YEKEAQHCLDESLVLPAYEWTLKCSHVFNTLDARGAVSVTERVGVMKRVRDLAVRCAH
                     QFLASREALGFPLLGEHA"
     gene            complement(1457..2074)
                     /locus_tag="VNN25_27495"
     CDS             complement(1457..2074)
                     /locus_tag="VNN25_27495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95351.1"
                     /translation="MTNRSLALIVAFALMACRGQDQPKRATPNYAVVSEGSADGVTST
                     INGPGEKPAPVTDTNADTTTNFTLPNNPDPLGNAATGTSVAGTLPSTPSYPASASTAP
                     VRRVSQPRTDTHGMASSSAPIAIDTIGSTTPPMAKETGTKPRSDSDADTTTASSDTTS
                     TDTTTSAGPGTTSSAPKKKTNAGKKPEDQPPPPPPPPPDTTGTQG"
     gene            complement(2164..3084)
                     /locus_tag="VNN25_27500"
     CDS             complement(2164..3084)
                     /locus_tag="VNN25_27500"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXH95352.1"
                     /translation="MNKMVTRLSLFVLMFLLVTMSASAQATRTWVSGVGDDVNPCSRT
                     APCKTFAGAISKTASGGEIDVLDPGGFGTVTITKPITIDGTGTFASSLASATTGFLIS
                     SSVANTSVTIRGISINGAGTTLGTNGIRVLTNAGATVFVHVEDCVIQNFSTAGINVAT
                     SAAASIFVRNSVIRNNAEGMHVVPTGGTVDLHIENCQIDQNSVWGVNADNNIKASIVG
                     TSLNHNLAGLLVSATTVEVDCDRCDMNFNTNGIFAGNGGSPVVRLTHCMITGNATNGV
                     FLNGGTVRGFTSNLIEGNGGNNTITTVAPQ"
     gene            3384..3857
                     /locus_tag="VNN25_27505"
     CDS             3384..3857
                     /locus_tag="VNN25_27505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXH95353.1"
                     /translation="MLKISKLTDYGLLAAVYLARHNGEVASAREIAGFYHLPVPMVTK
                     VLKMLHQSEVIESQRGVGGGYSFRGDAETVTLGYLIEALEGPWDLVECETTDDQGHAA
                     CAIRVACPSRRFMFGINRAVKGAFEQVTLGDLTRGVMPVAQIDKPMLSMRAESQL"
     gene            3871..5304
                     /gene="sufB"
                     /locus_tag="VNN25_27510"
     CDS             3871..5304
                     /gene="sufB"
                     /locus_tag="VNN25_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXH95354.1"
                     /translation="MTSTIEQIASQEYKYGFVTDIEADQAPPGLNEDIIRLISAKKNE
                     PEWMLEFRLKALRHWFTMKEPTWANVTYGPIDYQAIVYYSAPKQKKKLNSLDEVDPEM
                     RATFAKLGISLDEQKRLSGVAVDAVFDSVSVATTFRAELAKVGIIFCSFSDAVQNHPE
                     LVRKYLGSVVPYTDNFFATLNAAVFTDGSFCYIPKGVRCPMELSTYFRINSAGTGQFE
                     RTLIVADEGATVSYLEGCTAPMRDENQLHAAVVELVALDNASIKYSTVQNWYPGDKEG
                     KGGIYNFVTKRGKAFKNAKLSWTQVETGSAITWKYPSCILQGDNSVGEFYSVALTNHR
                     QQADTGTKMIHLGKNTRSTIVSKGISAGRGQQTYRGEVKIMKSAEGARNYSQCDSLLL
                     GDKCGAHTFPYIEVRNSTAKMEHEASTSKIGEDQLFYCQQRGISAEDAVSMIVNGFCR
                     EVFKELPMEFAVEAQKLLGVSLEGSVG"
     gene            5304..6062
                     /gene="sufC"
                     /locus_tag="VNN25_27515"
     CDS             5304..6062
                     /gene="sufC"
                     /locus_tag="VNN25_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXH95355.1"
                     /translation="MLDIKDLHAGIDGKEILKGLTLHVGVGEVHAIMGPNGSGKSTLA
                     NVLAGRPKYEVRSGSVLFQGKDLLTLPTEERSREGVFLAMQYPVEIPGVNNIYFLKAA
                     LNAVRKHRGEEELDAMEFLQLAREKMKLVELDEAFMSRGVNEGFSGGEKKRNEIFQMA
                     VLDPKLAILDETDSGLDIDALRIVAQGVNALRSEKHSVLVITHYQRLLRYLVPDFVHV
                     LADGRIVKSGGKELAEELEQKGYEWIEEEVAAAR"
BASE COUNT         1224 a         1807 c         1955 g         1232 t
ORIGIN      
        1 aaatcgtgat cggaagatgc gcgccgtcga gaacggagac gaccgagtga atcggacgga
       61 tgtaggagtg ctcgcctttg ccccatcgca tcatcttcgg ccagcgaagc gattcgacga
      121 tctgcggtat gcgttgctgc aggatctcat ccgccgcgcg gcctttgatc tgacgccgga
      181 tgcggatgta gtcgccgccc gtgatcaggt cctcaatcgc cgcgttgttc tttttcagga
      241 agccgttcag tgcctgcgtc ggctttcctt ccgcgtcata cgctgccttg atcggcgggc
      301 ctttgacttc ctcttcccgg tcgctctcgc gcaacggaat gcgggagaga aagaagatga
      361 tgcggcgcgg cgtgctgttg acgatgacgg ggttgcggtc gccggcaggc tcgccgaggt
      421 cgtgggtcag tttcgtcagt tgctggagca aagcagcgtg tgctgcttcg tgcatccacg
      481 cgggaatctc ttcggtgagc agctcgaaaa agtattcggc cattacgcgt gctccccgag
      541 gagcggaaat ccgagcgctt cgcgagacgc gaggaattga tgcgcgcagc gtaccgcgag
      601 atcacgaacg cgcttcatca cgccgacccg ctcggtcacg gagaccgcac cgcgtgcatc
      661 gagcgtgttg aagacgtgcg agcacttcag cgtccattca tacgcgggga gcacgagcga
      721 ttcatcgaga cagtgttgcg cttccttttc gtacaacttg aatagctgcc agtgcatgtc
      781 gatgtccgcg gtctcgaagt agtacttcga aaactcgtat tcatcctgct tgcgcaccgg
      841 cccgtagccg aagcctgctc cccactcgat gtcgtagacg ctcgactgac gcgaaaggaa
      901 catcgtcagg cgctccagac cgtatgtgat ctccgcggag atgggcgaca gatcgattcc
      961 tccggcctgt tggaagtagg tgaactgcgt gatctccatg ccgtcgagca gaatctgcca
     1021 gccgacgccc cacgcgccga gggtgggagc ctcccagttg tcctcctcga agcggatgtc
     1081 gtggcgggtg aggtcgaggc cgatcgcacg cagggagtcg agatagagat cctgcacgtt
     1141 caacggcgag ggcttgagga ccacctgaaa ctgatagtgt ttgccgaggc ggaaagggtt
     1201 gtcgccgtag cggccgtcgg cggggcggcg cgacggttgt acgtagccgg cgttccacgg
     1261 ttccggcccc agaacgcgca ggaaggttgc ggggtgcatc gtgccggcac caaccgcgac
     1321 gtcgagcggc gtttcgatca tgcagccgtg ttcggcccag aaggtctgga ggcgaaagat
     1381 caggtcttga aaggaaaggc gcggcatcgg gcgcgcattc taacgtggga gtgaggagtc
     1441 cgggagttgg agagcactat ccctgagtgc cggtcgtgtc tggcggcggc ggcggcggtg
     1501 gcggcggctg gtcttctggc ttcttgccag cgttcgtttt cttctttggt gcagaggacg
     1561 tcgttcccgg gccggccgaa gtggtcgtgt ccgtcgacgt cgtgtcggaa gaggccgtcg
     1621 ttgtgtcggc gtcggagtcc gagcgcggct tcgtcccagt ctccttcgcc attggcggcg
     1681 tcgtgctacc gatcgtgtcg atcgcgatcg gggcgctgga actggccatc ccgtgtgtgt
     1741 ccgtgcgtgg ttgggatact cggcggacgg gggcggtgct cgcgctggcc gggtagctcg
     1801 gagttgacgg cagcgttccg gcgacgctcg tgccggtggc tgcgttgccc agcggatcgg
     1861 gattgttcgg gagcgtgaag ttcgtcgttg tatcggcgtt tgtatccgtc acgggcgcgg
     1921 gcttctctcc cggaccgttg atcgttgacg tgacaccatc cgccgaaccc tctgagacaa
     1981 ctgcgtagtt cggcgtcgct cgcttgggct gatcctgacc ccggcaggcc atcagggcga
     2041 acgcgacgat aagcgcgaga gatcggttgg tcattgccca tgatccatcg catcgagcat
     2101 gccaataaaa aaccccttgc gtcacgcgac gcaaggggtt gaggctggca aacgggagcg
     2161 aaattactga ggtgcgacgg tcgtgatcgt gttgttgcca ccattccctt cgatgaggtt
     2221 actggtgaag cctctgacgg taccgccgtt caagaagact ccattggtcg cgttgcccgt
     2281 gatcatgcag tgggtcaggc ggaccaccgg ggaccctcca ttgccggcaa agataccgtt
     2341 cgtgttgaaa ttcatgtcgc agcggtcgca atcgacttcg accgtcgtcg ccgatacgag
     2401 caggcctgcc aggttgtggt tcaggctcgt accgacgatc gatgccttga tgttgttgtc
     2461 ggcgttcacg ccccagacgg agttctgatc gatctgacag ttctcaatat ggaggtcgac
     2521 cgtgccgcca gtcgggacca cgtgcattcc ctcggcgttg ttccgaatca ccgagttgcg
     2581 aacaaagatg ctcgccgcgg cgctcgtcgc aacgttgata ccggcggtgc tgaaattctg
     2641 gatgacgcag tcctcgacat gcacaaagac cgtcgcgcct gcgttcgtga gaacgcggat
     2701 gccgttagtg cccagggtcg ttccggctcc gttgatcgaa atgccgcgaa tagtgacgct
     2761 ggtgttggcg accgacgaac tgatgaggaa gccggtcgtg gctgaggcca gggacgaggc
     2821 aaacgtgccg gtgccgtcga tggtgattgg cttcgtgatc gtgaccgtgc cgaacccacc
     2881 cggatcgagc acatcgatct caccgccgga tgccgtcttc gagattgcgc ccgcgaacgt
     2941 cttgcaaggc gcggtgcggc tgcacgggtt gacgtcgtca ccgacgccgg aaacccacgt
     3001 acgcgtcgcc tgagcggaag cggacatggt gaccagcaag aacatgagta cgaaaagcga
     3061 gagccgggta accattttgt tcatacgatt tctcctcctg agtcctgatt gttagatgta
     3121 gatcgagcgg tgacgcccac gccaccgcaa ccgggctggt gtttggttgt ggaatttatg
     3181 tgtcttgcaa ccatcggcct gtgattccag tcacgatccc aaaaaaggat cagtcgctca
     3241 tcgctgattg aggttcgatc aatttagcac aaaagagtct cgcatgagta ccacatttga
     3301 cgggtggggg aaggtgtgag ccctcatggc ggttgtgcat accgtaccgg agtggtactg
     3361 tttcgctccc ttcgggaacc gcgatgctga aaatctccaa actcactgac tacgggctcc
     3421 ttgctgccgt ctatcttgcc cggcacaacg gcgaagtagc ctcggcgcgt gagatcgccg
     3481 gcttctatca cctgccggtt ccaatggtta cgaaagtgct aaaaatgctt caccaaagtg
     3541 aggtcatcga atcgcagcgc ggcgttggtg gtggctattc gtttcgcggc gatgcggaga
     3601 cggtgacgct cggatacctg attgaggcgc tcgaaggtcc ctgggatctc gtcgaatgcg
     3661 agacaaccga cgaccagggc catgccgcct gcgcgattcg tgtagcgtgc ccctcgcgcc
     3721 gcttcatgtt cggcatcaac cgcgcggtca agggagcctt cgaacaagtc acgctcggcg
     3781 atctcacccg cggggtcatg ccggtagcgc agatcgacaa gcccatgttg tccatgagag
     3841 ctgagagtca attatgaatt gcagggaatc atgaccagca ccatcgaaca gattgccagc
     3901 caggagtaca agtacggctt cgtcaccgac atcgaagccg atcaggcgcc gcccggcctg
     3961 aatgaagaca tcatccggct catctccgcg aagaagaacg agcctgagtg gatgctcgag
     4021 ttccggctca aagcccttcg tcactggttc accatgaaag aaccaacgtg ggccaacgtg
     4081 acgtacggac cgatcgacta ccaggcgatc gtctactact cggcgccgaa gcagaaaaag
     4141 aagctcaact cgctcgacga agtcgatccg gagatgcgcg cgacgttcgc aaagctcggc
     4201 atctcgctcg atgagcagaa gcgcttgtcg ggtgtcgccg tcgatgcagt cttcgactcc
     4261 gtctcggtcg cgacgacgtt ccgcgccgag ctggccaagg tcggcatcat cttctgctcg
     4321 ttctccgatg ccgttcagaa tcaccctgaa ctcgttcgca agtacctcgg atcggtcgtt
     4381 ccctacaccg acaacttctt tgccacgctc aatgccgctg tcttcaccga tggctcgttc
     4441 tgctacatcc cgaaaggcgt tcgctgcccg atggagctgt cgacctactt ccgcatcaac
     4501 agcgccggca ccggccagtt cgagcggacg ctgatcgtgg ccgatgaagg tgccaccgtc
     4561 agctatctcg aaggctgcac cgccccgatg cgcgatgaga atcagttgca cgccgcggtc
     4621 gtcgagctcg tcgccctcga caacgcgtcg atcaagtact cgacggtgca gaactggtat
     4681 ccgggcgaca aggaaggaaa gggcggcatc tacaacttcg tcaccaagcg cggcaaggct
     4741 ttcaagaacg cgaagctttc ctggacgcag gtggaaaccg gctccgcgat cacctggaag
     4801 tacccgagct gcatcctgca gggcgacaac tcggtgggcg agttttactc ggtcgcgctg
     4861 accaaccacc gccagcaggc tgataccggc acgaagatga ttcacctcgg caagaacacc
     4921 cgctcgacga tcgtgtcgaa gggaatctcc gccggccgcg gacagcagac ctatcgcggc
     4981 gaggtgaaga tcatgaagtc ggctgaaggg gcgcgcaatt actcgcaatg cgactcgctc
     5041 ctcctcggcg acaaatgcgg cgcgcacacg ttcccctaca tcgaagttcg caactcgacg
     5101 gcgaagatgg agcacgaagc gtcgacgtcg aagatcggcg aagaccagct cttctactgc
     5161 cagcaacgcg gcatctccgc ggaggacgcc gtctcgatga tcgtcaacgg cttctgccgc
     5221 gaagtgttca aagaattgcc gatggagttc gcagtggaag cacagaagtt gttgggagtc
     5281 tcgttggaag gatcagtcgg ttaatgctcg acatcaaaga cctgcacgcc ggaatcgacg
     5341 gcaaggaaat cctcaaaggc ctcacgctgc atgttggcgt aggcgaggtg cacgcaatca
     5401 tggggccgaa cggctccgga aaatcaacgc tcgcgaatgt tctggcggga cgcccgaagt
     5461 acgaagtgcg ctcgggaagc gtgctgtttc aggggaagga cctgctcact ctgccgaccg
     5521 aagagcgatc gcgcgaaggg gtgttcctgg ccatgcagta tccggtcgaa atccccggcg
     5581 tgaacaacat ctacttcctc aaggccgcgc tcaacgccgt caggaagcac cgcggcgagg
     5641 aagagctgga cgcgatggag ttcctgcagc tcgcacgcga gaagatgaag ctcgtcgagc
     5701 tcgacgaagc attcatgagc cgcggcgtca acgaaggatt ctcgggcggc gagaagaagc
     5761 gcaacgagat cttccagatg gccgtgctcg atccgaagct tgccatcctc gacgagaccg
     5821 actccggcct cgatatcgat gcgcttcgta tcgtcgccca gggtgtcaat gcccttcgca
     5881 gcgagaagca ttcggtgctc gtcatcacgc actaccagcg tctgcttcgt tatctcgtcc
     5941 ccgacttcgt tcatgttctc gccgacggca ggatcgtgaa gtcgggtggc aaggagctgg
     6001 cggaagagct cgagcagaag ggctacgagt ggattgaaga agaggtcgcg gcggcacgat
     6061 gagcagcgtt cttgatcgcg agatcccgct ctccgccatc gagccggaac acatcgatgg
     6121 cgagccggag cgcgtccgcc gcatccgttc gcaggcagcg gagcgcttca acgcccttgg
     6181 ctggccgacc acacggctcg aggagtggaa gtacacct
//
LOCUS       DATLVW010000929         6209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_575501, whole genome shotgun sequence.
ACCESSION   DATLVW010000929 DATLVW010000000
VERSION     DATLVW010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575501"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(574..1800)
                     /locus_tag="VNN25_27520"
     CDS             complement(574..1800)
                     /locus_tag="VNN25_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXH95356.1"
                     /translation="MTTNGTTAGTMPEIAEHEPAIAPSKAKRLYLILGGAVVLLLIGY
                     LLYAFMSAGKESTDDAQVGADIVPIAARVAGQVTAVYIVENQPVKAGQPIADLDPSDA
                     QVKVAQAEGDLATAQAQAADADARTAVTSANARGALTSAQGALQSSKESVDTSAASIN
                     EAHAAVTRATANAQKARLDFQRAEELGGKGDISKAQVDAARAAHESADADLAQARARV
                     SEAENSRQAAQANVQTAQGRVQQSAPVQEQITAAQAQARLAHARIATAEAALHAAQLN
                     LSYTKIIAPRDGIASKLAVHAGSYVTAGMPIVQLVPQTTYILANFKETQMAGMRPGQR
                     ATVRIDALGRRDFEGKVESLSGGTGASFSLLPPDNASGNFVKVVQRVPVRISWNGPPA
                     DQVAVGSSAEVTVFTK"
     gene            complement(1801..3429)
                     /locus_tag="VNN25_27525"
     CDS             complement(1801..3429)
                     /locus_tag="VNN25_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXH95357.1"
                     /translation="MSSAAATFDAAGSFPARPERKPVNKWLVTVSITFGTLMGAIDAS
                     IVNVAVPHLTGSLGVTIEQITWVTTGFVIATVMVMPLTGFLARLFGQKRVYMACLALF
                     VIGSALCGMVRTLPLLVFFRILQGLGAGALQPTEQAILRQTFPPKEQGMAMALFGMAV
                     VLGPAFGPTLGGYIVDNYSWPWIFYINVPIGIISLVMVSRFVHEPEDVRAAMQQMAVR
                     QRKNMDWGGIALLFLGLGTMQYVLEEGGRNDWFQSGEIKIIALVAIVSLLALVIRELS
                     ARVPAVDFSLFKDVVFASGTLIGAVMFAMLLAVTFLLPLFMQFELGFSATQAGLAMMP
                     RSLCMLVVMPIVGRLYNKVSPRLVVAFGILLFAYTAWLMGHYTLASGTVDVVKVLIIQ
                     GVAFSCLWIPLTTMSLSTIPRHRMSDATGLNSLLRQTGGSVGLAVFATLLARYATRAR
                     GDMLPHFSMSSPSMIARMGGIQRMLQARGLDAMAARNAAARMIDGGLRQQAMMLSFEK
                     LFYLSGILFLCVLPLVFFLKMPKSAEKIDVHVEM"
     gene            complement(3426..4763)
                     /locus_tag="VNN25_27530"
     CDS             complement(3426..4763)
                     /locus_tag="VNN25_27530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXH95358.1"
                     /translation="MLSKRLSLLLLAVTFSASAQTPAQPLRLTLEDAIHMALGNGTQA
                     ELARSNEQMARIARSEALSALMPQADVKLMQYSQSINLATFGFSLPGLPPVIGPFNVT
                     DGQLSAAVQVFNFAALRRYQSLKSSAEASHYQNEQAENDVAAAVARLYLIAQRAQSQI
                     DARQADVALFTRLLQTAQDEFKAGTGTRLDVAQGNVQLARARQALLIAQNDRQNATLA
                     LLNAIGADESSDVIFTALTSAPTSVPDLDPSLTTAKEHRPELRQATEQVRAAQLTVEA
                     ARARLLPSVSLDFEGDMSGNRTNDLHWTRRIAANLGVPLFRADINANIARAKVMLHDA
                     ETQLSQRQRDVEQDVRRSVMSLQNAESRVAVATESVTVAEEALTVAGDRRAAGYGSPV
                     EVDRAQDAYRQAHEDLIAAQADAAAAQFDYEHATGGIRRYITNASNSAPEGKP"
     gene            complement(4989..>6209)
                     /locus_tag="VNN25_27535"
     CDS             complement(4989..>6209)
                     /locus_tag="VNN25_27535"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXH95359.1"
                     /translation="WNEFWAAPERCAAVRAHSDGEPDPNSPIDPKEFAIGSEDWIEAK
                     HAWEAFWAPKSEALLHYLEEAADIQSEPVPPSSGTAKLSSIRQKLNKIRALNRKWGCP
                     SEPWASVSPNLLWNIANIPASQVSMIGRIVGPAFAPVAACASFGVALKLAADAIQLGE
                     AKAVVVGMTDPPPHPLVISAFYNANVLSADADISRPLTALKGTHIAGGSCVWIVGDAM
                     ELMAQGFRPVGMEIVGIGTSSDAHHIITPSKGGPQLAMKQAMEEVQAADVTTWDMHAT
                     ATPGDALEVENSLEMLHPDVLFTARKGTFGHGMSVGGGWELTAQHLGMAKGRLYPMAL
                     TEGELHADVKIQEAKFVSSDGCDNVGQGYSGKLSMGVGGINSCVISKPWDAKMIEENV
                     RKADVVPALAGKLD"
BASE COUNT         1071 a         1887 c         2076 g         1175 t
ORIGIN      
        1 ccttctcaaa gtgacggcgc tgggaagcag taaaaccggg agcgtcgccg aatgacgatg
       61 tgacgtttgt cgtgatgcga gcgaagggct aaagagtagc gcaggcattc ttgcctgctc
      121 ccgtcgggca ttcttgcccg gcggtgtctt ctgaccatcc gagaatcccg agattcccgc
      181 ggcgagccac tcgatcatta ttgtgcaatg accccttgca caatttaagc gcctggcgag
      241 gtgggcggcg aacatcggaa ttgtgaaaca aggtgtttca ctttctcgac cgccacagtt
      301 ctggctgccc gattcatagg gagtgttttt caggttccgg aggtggtcac agactgtgac
      361 cacctcatcc ttcaaggtca cagattgtga ccttgaccgc cgcatctcgt cgtcctcagc
      421 gcctgtggaa cgcgacgtcc cgagttttct gaacgcgaag cggcgattca ggcgcgcgtg
      481 ctgctgttct tgagggttcg ccagcgagcc gctggcggta cagccgtcga gccgacggcg
      541 ctacgaagcg cgaaactact tcggacgcag cccctacttc gtaaacaccg tcacttccgc
      601 cgaggagccg accgccactt gatcggctgg tgggccgttc caggagatgc gcactgggac
      661 gcgctggact accttcacga agttgccgga ggcgttgtcg ggggggagaa gggagaacga
      721 ggcgccggtg ccgccggaga ggctttctac tttgccttcg aagtcgcggc ggccgagggc
      781 gtcgatgcgg acggtggcgc gctgaccggg gcgcatgccg gccatctgcg tctctttgaa
      841 gtttgccagg atgtacgtcg tttgcggaac gagctggacg atcggcatgc ccgcggtgac
      901 gtaggagccg gcgtgaacgg cgagcttgga ggcgatgccg tcccgagggg cgatgatctt
      961 cgtgtaggaa aggttcaatt gcgcggcgtg aagcgcggct tcggcagtag cgatgcgtgc
     1021 gtgcgcgagg cgagcctgcg cctgtgctgc cgtgatctgc tcctgcacgg gtgcactctg
     1081 ctgcacgcgt ccctgtgcgg tctggacatt ggcctgtgca gcctgacgac tgttttcggc
     1141 ctcgctgacg cgggcgcgtg cctgcgcgag atcggcgtcg gcggattcgt gcgccgcgcg
     1201 tgctgcatcg acctgcgcct tcgagatatc tcccttgccg cccagctcct cggcgcgctg
     1261 gaagtcgagc cgcgctttct gcgcgtttgc ggttgcacgc gtgactgcgg catgcgcctc
     1321 gttgatcgac gccgcggaag tgtcgacgct ctccttcgag ctttgcagtg caccttgcgc
     1381 cgaggtgagt gcgccgcgcg cgttcgccga agtcactgcc gtgcgtgcgt cggcatcggc
     1441 agcctgtgcc tgcgcggtcg ccagatcgcc ctcggcctgc gcgactttca cctgcgcgtc
     1501 ggacggatcg aggtcggcga tcggttgacc ggctttgacc ggctgattct cgacgatgta
     1561 gaccgcggtc acttgtccgg ccacgcgcgc cgcgatgggc acgatatcgg cgccgacctg
     1621 cgcatcgtcg gtactctctt taccggcgga catgaacgcg taaagcaggt agccgatcag
     1681 cagcagcacg acggcgccgc cgaggatgag atagaggcgt ttcgccttcg agggcgcgat
     1741 tgccggctcg tgctcggcga tttccggcat cgtgccggcg gtggttccgt tggtcgtcat
     1801 ctacatctcc acatgaacgt cgatcttctc cgcactcttc ggcatcttca ggaagaagac
     1861 gagaggcaga acgcatagga acaggattcc tgacagataa aacaatttct cgaacgagag
     1921 catcatggcc tgctgccgaa gtccgccgtc gatcatgcgc gcggcggcgt ttcgcgcggc
     1981 catggcatcg agcccgcgcg cctgcagcat tcgttggatg ccgcccatgc gcgcgatcat
     2041 cgacggactc gacatcgaga agtgaggcag catgtcgccg cgggcgcgcg tggcgtagcg
     2101 ggcaaggagc gtcgcaaaca cggccagccc aaccgagcct ccggtctgcc gtagcagcga
     2161 gttcagtccg gtggcgtcgg acattcgatg acgcgggatc gtgctgaggg acatcgtcgt
     2221 cagcggtatc caaaggcaac tgaatgccac cccctggatg atcagaacct tgactacgtc
     2281 aacggtcccg ctggccagcg tgtagtgtcc catcagccag gccgtgtagg cgaagagcag
     2341 gatgccgaag gcgacgacca gacgcggaga aaccttgttg tagagccgcc cgacgatcgg
     2401 catcaccacg agcatgcaga ggcttcttgg catcatcgcc agacccgcct gcgttgcgga
     2461 gaaaccgagc tcaaactgca tgaatagcgg gagcaggaac gtcaccgcca atagcatggc
     2521 gaacatgacg gcgccgatca gcgtgccgga cgcgaacacg acgtccttga agagcgagaa
     2581 gtcgactgcc ggcacccgcg cactcagttc gcgaatgacc agagcaagga gcgacacgat
     2641 ggcgaccagc gcgatgatct tgatctcacc agactggaac cagtcgttgc gtcctccctc
     2701 ctcgaggacg tactgcatgg tccccaggcc gaggaagagg agcgcgatgc cgccccagtc
     2761 catgttcttc cgttgacgta ccgccatctg ctgcatcgcc gcgcggacgt cctcgggctc
     2821 gtgcacgaag cgcgagacca tgacgagcga aatgatcccg atggggacgt tgatgtagaa
     2881 gatccacggc cacgaatagt tgtcgacgat gtatccgccg agcgtggggc cgaaggcggg
     2941 gccgagcacg accgccatcc cgaatagcgc catggccatc ccttgctctt tcggaggaaa
     3001 ggtctggcga agaatggcct gctcggttgg ctgcaaggcg cccgccccaa gtccctgcag
     3061 gatgcggaag aaaacgagga gcggcagcgt gcgcaccatc ccgcaaagtg ccgatccgat
     3121 gacgaagagc gccaggcagg ccatgtagac gcgcttctgg ccaaagagcc gcgccagaaa
     3181 tcccgtcagc ggcatgacca tgaccgtggc gatgacgaac ccggtcgtga cccaggtgat
     3241 ctgttcgatc gtgacgccga gggagccggt gaggtgaggg acggcaacgt tgacgatgga
     3301 ggcgtcgatc gcgcccatca gcgtcccgaa tgtgatcgag actgtgacca gccatttgtt
     3361 gaccggcttg cgctccggcc gtgcgggaaa gcttcccgcg gcatcaaacg tcgctgcggc
     3421 ggaactcacg gctttccctc gggagcgctg ttgctcgcgt tcgtgatgta gcgacgaatg
     3481 ccgccggtgg cgtgctcgta gtcgaactgc gcggctgcgg catcggcctg cgcggcgatg
     3541 agatcttcgt gcgcctggcg ataggcgtcc tgggcgcggt cgacttcgac cggcgagccg
     3601 tagccggctg cgcggcggtc gcctgcaacg gtcagcgctt cttcggcgac cgtcacgctt
     3661 tctgttgcga cggccacgcg cgactcggcg ttctgcagcg acatgacgga gcgtcggaca
     3721 tcctgctcga cgtcgcgctg gcgttgcgac aactgcgttt cggcatcgtg caacatcact
     3781 ttggcgcggg cgatgttggc gttgatgtcg gcgcggaaga gcgggacgcc gaggttggcg
     3841 gcgatacggc gcgtccaatg gagatcgttg gtcctgttgc cgctcatgtc gccctcgaag
     3901 tcgagcgaga cactgggcag gaggcgcgca cgggcagcct caacggtgag ttgcgcggcg
     3961 cgcacctgct cggtggcctg acgcagctcg gggcgatgtt ctttggcggt cgtcagggag
     4021 ggatcgagat cgggaacact cgtcggggcg gaagtgaggg cggtgaagat gacgtcgctc
     4081 gattcgtccg cgcctatggc gttgaggagc gcgagcgtcg cgttctgccg gtcattctgg
     4141 gcgatgagca aggcctgacg ggcgcgcgca agctgcacat tcccttgtgc gacgtcgaga
     4201 cgggtgccgg tgcccgcttt gaattcatcc tgcgctgtct gcagcagccg cgtgaagagc
     4261 gccacgtcag cctgacgcgc gtcgatctgc gactgcgcac gttgcgcgat cagatagagc
     4321 cgtgccaccg ctgcggcgac gtcgttctcc gcctgctcgt tctggtagtg actcgcttcg
     4381 gccgagcttt tcagcgactg gtagcgccgc aaggccgcga agttgaagac ctggacggcc
     4441 gcggagagct gcccatcggt gacattgaag ggaccgatga ccggcggtag tccgggcaaa
     4501 gaaaagccga acgtggccag gttgatggac tggctgtatt gcatcagctt cacatccgcc
     4561 tgcggcatca gcgcgctcag cgcttcactg cgcgcaatgc gcgccatctg ctcgttcgac
     4621 cgcgctagct ccgcctgggt cccgttgccg agggccatgt ggatggcgtc ttcgagcgtg
     4681 aggcggagag gctgcgccgg cgtctgcgcg gaagcgctga acgtcactgc aagcaagagg
     4741 agggagagtc gtttcgaaag catgagcttg aagcgccgct gctgtggtgc aatcccgtcg
     4801 ccagcgcgcc gactgaccgt tcggtcagtt cgcgacgtaa aggagatgag ggcggcgttt
     4861 cttcccgtgg cgcagcttgt ttgaggtagc gaacgaaact cttgtcacca cagaggcaca
     4921 gaggtcacag agtgagtgtt cgactctgtg acctctgtgt ctctgtggta atcccttcga
     4981 tcgaaccttc agtccaactt gccggcaagc gcaggaacga cgtccgcctt gcgaacgttc
     5041 tcctcgatca tcttcgcgtc ccacggcttg ctgatcacgc aggagttgat gccgccgacg
     5101 cccatgctca gcttgccgct gtagccctgg ccgacgttgt cgcagccgtc gctggagacg
     5161 aatttcgctt cctggatttt cacatcggcg tgtagctcac cctcggtcag cgccatcgga
     5221 tagagacggc ctttggccat gcccagatgt tgggcagtga gctcccagcc gccgccgacc
     5281 gacatgccgt ggccgaacgt tcctttgcgt gcggtgaaga gcacgtcggg atgcagcatc
     5341 tccagcgagt tctcgacttc cagcgcgtcg ccgggtgtgg cggtggcgtg catgtcccag
     5401 gtcgtgacgt cggcggcctg cacttcctcc atcgcctgtt tcatcgcgag ctgcggaccg
     5461 cctttcgacg gcgtgatgat gtgatgggcg tccgacgacg tgccgatgcc gacgatctcc
     5521 atgccgacgg gacggaatcc ctgcgccatc agctccatgg catcgccgac gatccagacg
     5581 cacgacccgc cggcgatgtg cgttcctttg agcgccgtca gcgggcgcga gatgtcggcg
     5641 tcggcggaga ggacgttggc gttgtagaag gcggagatga cgagcggatg cggcggggga
     5701 tcggtcatgc ctacgacgac cgctttcgct tcgccgagct ggatggcgtc ggcggccagt
     5761 ttcagcgcca cgccgaatga ggcgcaggcg gcaaccggtg cgaacgctgg cccgacgatg
     5821 cggccgatca tcgacacctg cgatgccgga atgttggcga tgttccagag aaggttcggc
     5881 gagacgctgg cccatggctc gctcgggcat ccccacttcc gattcagcgc ccgaatcttg
     5941 ttcagcttct gacggatcga gctgagcttc gcggtgccgg aggatggcgg caccggctcg
     6001 gactggatgt ccgccgcttc ctcgagatag tgaagcagtg cctcgctctt cggtgcccag
     6061 aacgcttccc aggcatgctt ggcttcgatc caatcctcag agccgatcgc gaattctttg
     6121 ggatcgatcg gggagttggg atcgggctca ccgtcgctgt gtgcgcgcac ggcggcgcat
     6181 cgctccggcg cagcccagaa ctcgttcca
//
LOCUS       DATLVW010000930        19128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_165374, whole genome shotgun sequence.
ACCESSION   DATLVW010000930 DATLVW010000000
VERSION     DATLVW010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165374"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3924)
                     /locus_tag="VNN25_27540"
     CDS             complement(<1..3924)
                     /locus_tag="VNN25_27540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95360.1"
                     /translation="MADVEKQIVQVVVAVLGSLTPILIEFLKRRLSAAKDDTLGPRAT
                     GKSRSLLDLAILLAYSAFLSAILCHSIIVSGQAPSFDGMVLIGSACIAAGCAFGLYLA
                     WRRGIGEMAAGAMSLASLIVLLIAPGSSKTATQLHGVEPGLPLLLPLLALTFATAAAL
                     MYVSGNPLARDVTPARRRGVTLGLIALGLSTAIILGYDFVNDTIADEKAPKFEGSDDQ
                     KHSAEAALAQLRSLDLWNRGIFYRVASESILSDEYRRQYFEIVTEINAFEKKAAEAAA
                     AQTATLNATVSARGDGANALVGGADNDGQRSTPNQLSTALQAAGRETLKRMRFEWLKT
                     LESHVDKLDTPSLTDYLATRLPFIHPTPLMATTKQVPLSMPGLTASDRYRTVSEYRLL
                     YAFPTASAIPWDAAAFVYPSTVVASLRLRDGTMISGNFPAPPDARRAIERARIADMLF
                     PKTTDNAFNRHLRMQMALPQREEGFLAYRAALRLAVKERPTELESQLDAFDSLTPEQQ
                     SAFSEYVARSTPDLPVFDLLKSLADGKPKLDGFPTSAAARLDLSNHLRRNEADPCIAD
                     LGASVPVCKELVRIFQKNDTDRAQLAKLISDSDRNDISIAPLFSPGVLDFVTSTHALR
                     NSKEFLQIISDPVTPAMRVILKTPSYLSLSEKFETFRALTPANREQLLLHAAIMLYRA
                     SGKDSLAPIRVAALQANTLSPTLACLFATMLMLPGSVIAIAFGGFTARKLVERDRTRL
                     LVNAETQAGESTGDIGLPVDLLGREPLLDHVRKLSGRGWSTIALVGRRGIGKTRILNE
                     IYKPSEPTPADPAVSVWVAAPTNYEEADFVESLFERLALQTEHAVGKELGAKPLQVRL
                     LETKQASAGTWMFATTMAAFGVLVMFAFGEKARPELRMACMPVLVVAAMATLALVRYL
                     ASVQPVDLSPWLERDRSRTASIVLIYREARTALQYLVIRRGRAGAPTLPMGLINAVAI
                     ALGVTIGTLIGFIVWGYQDTSTTSEALFSIGCGVAAAVVATFAFRLAATASNRGDQSG
                     SSMMSLTSNYRRFAETVVRHVDAGAFGSRRRVMVCIDELDKIVEQKSVGEFLRKMKGI
                     FEVPGVYYYVSLAEDTLAGLYLASADGKNEIDSSLDHIVRVPQLGWNDCKEMVRAYCK
                     RRGFSDVGEQLLTVIAAVSYGVPRDVIRQCDEMLAGYIGRTDGKLVIENTAAIAAAVR
                     RDRVEIACERQGWPIETRDKLLSTPDQAATAILEMLRDEPKESVKRLLAMLWTLCASE
                     TAALLGGQTAEKQSELIFRLGYDVSSRPIVDILAAISQISIELTST"
     gene            complement(4109..4336)
                     /locus_tag="VNN25_27545"
     CDS             complement(4109..4336)
                     /locus_tag="VNN25_27545"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH95361.1"
                     /translation="MAVTPTIGELLGERVSQLRKKRALTQFQVSERCGVPQSRISAIE
                     KGSHVPNVETAIRLPWALGRKVTAPMSVFDV"
     gene            4431..4616
                     /locus_tag="VNN25_27550"
     CDS             4431..4616
                     /locus_tag="VNN25_27550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95362.1"
                     /translation="MHVIEEALGDDADGILPQAIRSAELHVVDEKPYPQMTQMERANG
                     FHLRSISVICGSFSRVT"
     gene            5889..6461
                     /locus_tag="VNN25_27555"
     CDS             5889..6461
                     /locus_tag="VNN25_27555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95363.1"
                     /translation="MSTTTPNPGTPEAGTPAPSPQTLDAQERIARLRVIADDFPDEAS
                     PKPLTTAQRQLASRTSIVFLEKAALFAEAAPNIGVALSVDTAALRDAIASELATGGVV
                     DETRTLISRMEDANTRKKLKAVKVARDVYRVAKSFVTTDLGHVVTPHVDEMKRALQRP
                     RAKKKAAPAEPPATTPAVTPNPSDSTSQQK"
     gene            complement(7034..8452)
                     /locus_tag="VNN25_27560"
     CDS             complement(7034..8452)
                     /locus_tag="VNN25_27560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95364.1"
                     /translation="MNRSSLVGLFSLVLLMVVAASLVGSVWFLLPQLTPVAALRTVAT
                     WFDPPFFGYNLLLLFFAVLFPALMAISYTFSMGKEKTRRIRRELGALWEPNRVWIEEY
                     IAKQFHWRDYVVAVSGMATVVAFGAAVLLLLKPVGGAGDMPRPGLDFSRGANLLLLGP
                     YVENYTKDGHFHMVAHSLTAFAFGFLGAYIYSIAQLIRGYFCVDLSPSAFVSAAVRIT
                     VASTTALVAAFGLSILFAEQDTAAAAGKAAFNVVSSVGNVTGIHRSYEQMAALLPVLG
                     FFFGYFPSRALKTIQHLANRVLRNAEGPGYGSTNLNKVSGMNGSHAFRLEREGVDNVE
                     NLATSEPIEIALRTGFSYPQVKTWVGEARLRTHLARHFVPFVERTGIRTFDQLTAFVQ
                     RWDKNARGSAYVFLADMMRADGETKQRIAAKLEAMVMLHDLIDPPIDAEARSENAQTE
                     PSPLVSNTLPIRRVSSSSIQAS"
     gene            complement(8854..9165)
                     /locus_tag="VNN25_27565"
     CDS             complement(8854..9165)
                     /locus_tag="VNN25_27565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95365.1"
                     /translation="MIEIVFNAVWLAVAVAAFVFVPRRSGKALLALAAILVLLFPIIS
                     VSDDLALDRNSLEEALAIIVAFLTLFVGLVAFARVEPRLLQPALVLLATPSDPRSPPR
                     A"
     gene            complement(9221..10231)
                     /gene="glnII"
                     /locus_tag="VNN25_27570"
     CDS             complement(9221..10231)
                     /gene="glnII"
                     /locus_tag="VNN25_27570"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HXH95366.1"
                     /translation="MAKITAEYIWIDGQKPTAKLRSKTKIIDSVKSLTDIPEWGYDGS
                     STEQATGHFSDLLLRPVKMMPDPLHRDGDVLVLCEVLNPDGTVHPSNTRAFLREVAER
                     YKEHDCWFGIEQEYTLFEGNRPLGWPASGFPAPQGGYYCGVGADEVFGRDLVEAHADA
                     CIRAGIHIVGTNAEVMPAQWEFQVGAVQAPDIADELWLARWLLYRIGEEFGISATLYP
                     KPVKGDWNGAGAHTNFSTKETRAEGGIKVIEEAMKKLAKRHDEHIKVYGAHNEERLTG
                     LHETAPIHEFRYGVSDRGASVRIPMATTNAGRGYFEDRRPAANMDPYQVCAKLIETVC
                     GP"
     gene            complement(10337..11485)
                     /locus_tag="VNN25_27575"
     CDS             complement(10337..11485)
                     /locus_tag="VNN25_27575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXH95367.1"
                     /translation="MIQFHALEIPQLERFLERQKAGLSRKSDVDLDLILRQILQKANE
                     FVPSESGSILLDDPFRKVAVRRENELVFIATFGGSSRDLIGQRLSAKFGIVGQVYQTG
                     TPYLSADVKEDDFFYSEFDQQLGHKTHSVVCVPIYIGKTVCGVLELINRLEGSIFTER
                     DKTLLEIFAGYTSFTLQNALDAKRAHELAKKDDLTGLYNDRWFNVRLHDSIAEARKSG
                     REGVLIFMDLDRFKAINDSHGHLAGSQVLREVGFLLKRIVPQEEATVARYGGDEFVIL
                     LPNTTLEEGMAVCEEIRQTIEQTVFLDREWGFSMQPLRLRGLLSASLGIAQHVPDTDP
                     NRSLELEQTDLLRRADSAMYQAKSLGKNRVVVSRVGQELQSASEASRV"
     gene            11594..12472
                     /locus_tag="VNN25_27580"
     CDS             11594..12472
                     /locus_tag="VNN25_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXH95368.1"
                     /translation="MRKLFVVIAALSATSLFAQQDFSKVEIKVTKVAGTVYMLAGAGG
                     NIGVSVGDDGIVIVDDEFAPLAPKIREALHGITATPIKFILNTHYHGDHTGGNEVFGH
                     DGPIIAHENVRKRLASGSKAAGRETPPAPKDALPVITFNDRATLHVNGEDIRAVHMPH
                     GHTDGDAVIYFTQSNVVHMGDDFFNGMFPFVDVDNGGSVRGMLASVDNVLKTLPDDAK
                     VIPGHGPLSDKAGLRAFAEVLRGTSNAVAAALAAGKSLDQMKQENILAPWDSWGQGFI
                     KSDAWLALLVSEAKKQ"
     gene            complement(13197..14720)
                     /locus_tag="VNN25_27585"
     CDS             complement(13197..14720)
                     /locus_tag="VNN25_27585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95369.1"
                     /translation="MISRIAAIVRADFLIRFRRASTVIVFLLLSGFAYVWVPATSTGR
                     TLMQIHGARVLYNSAAIGMGTSMLASMFIGLFGFYVISNAVRRDVDSRCGYVIASTNM
                     RTSEYVLGKFLGNVVFLSTFVFGFMLVSMAMQVVRGEAPLEPLLFLKQYAIVTPSTIV
                     FVSVVAIVFESIPWLSGRIGDVLYFFFYCGSLGIVASSMEHGGGGVLRYFDFSSFGYL
                     FEQIRHNFHTTSMSIGSSSFNRAMPPVTIAGLNLGGGAWATRIVSTVTPIPLLVFAQL
                     FFHRFDPARLRAAGAKGKRGWMRNFNALARPFGRPLAAIPVRGAALKDAMMTFSSTPF
                     TIFALIGISIASLAAPDSLPITFGIVAIFIADVASRDRRAGTTGLIYAAPRLRERFVT
                     WKLTSSVLVATLLLIAPVVKIAIAHPIRLPALLIGIAFVAASATALGIISGNPKTFIV
                     GFLTFWYVVVNDHGQTPAFDFAGFFSTPAMRVTAMYAAIAIVLLATAEVVHRARLRA"
     gene            complement(14717..15580)
                     /locus_tag="VNN25_27590"
     CDS             complement(14717..15580)
                     /locus_tag="VNN25_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH95370.1"
                     /translation="MLHLEGVSKRFRGGNYGVRDLSLSIGSGVLGLLGPNGAGKTTLM
                     QMIATITRPTAGTIQFDGVDVARNPNEIRRRLGYLPQDFGVYDNLTAHEFLSYFAALK
                     GVHSRARINEMLEMVNLHNVAKRPVGGFSGGMKQRLGIAQALVNDPDLVIVDEPTAGL
                     DPEERVRFRNVLADAGLGKLVILSTHIVSDVESVATHIAIMNGGSIVACATPEELMRG
                     AEGSVWELVVPSERFDELRRTARVSSAVRKSDGVHVRIVSGDEPRGGATAAEPTLEDA
                     FLYTMREKAAA"
     gene            complement(15555..17879)
                     /locus_tag="VNN25_27595"
     CDS             complement(15555..17879)
                     /locus_tag="VNN25_27595"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXH95371.1"
                     /translation="MSSFAALRRPRMTLALYLATAIAVLALAHRYITRLSRGVALVLL
                     LLPLVFTGRGLLTGRVYAPVELTYSSEPLYAHQSELAVPDPQNRMLSDIAFQMIPWRQ
                     AVRHSIGHGEWPLLNRFEGCGDILAGEAQPAPFSPFTLIALILPVAASFGFTASMAFF
                     LAALGAFLLARDLECSEMASLVAAAVFAFGAPIALQILWPLGFCWALFPLILLATRRV
                     VAVPSIRSVVILVVALTLEALAGHPETLLHVTAIGAAYGVFELARSDGKQRVRAFGYA
                     VGAGIIALLLSAIAILPMLDASNQTVEHRIRTEFFAPGTLRVAAGLPRAALLSDLLPF
                     ARNSFRNFPMARAEAGSIALALAIFALWRGRGRERWFFATLGFLTLLAGANAWPVAQL
                     LHRLPLFNLALNDRLASAVPLCLAILAALAIDALSDRAGIVMVGLLVALAVPASAMRA
                     GSIDMPRFVAEVVPLALAAIVVLVVANRKIAIPILFALILLQRTISDGALVPANDARI
                     AYPQLALFQPLQNIHERFRIVATGPGLFPNTATMYGLEDARVLTAMTLTSYDETFPAW
                     CRRGGFGFNQIDDLTRPMLSMLNVRFAVVPDGDPIPDGWCSVVADHGSRLVENMRVLP
                     RAFVPRNIRAGVANDVEDMVAATDFSELAWLDLPGAARDLANGPGSVAIHPRKLGFDL
                     DAVMTRDGFVVISETAWRGWRAYVDGKRVKIVRANHAFLGVFVPAGRHKVRVVYLPQA
                     FVTGRMISLITLALLITAVLALRIGAHASSRRRQ"
     gene            complement(17843..18745)
                     /locus_tag="VNN25_27600"
     CDS             complement(17843..18745)
                     /locus_tag="VNN25_27600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95372.1"
                     /translation="MTQMNADGNICVCLRHLREKKALPTLEQKNAATLWMNNDFSRKN
                     ATSRRVNATTRHVNARTRPMNATASRMNATAGHLNAANMADERESTPDKRDCMAHKRD
                     CTPVCRESTAVCRDSSACCLGIRPLVGLALPSAATVCRLLAGIQPAGPRYAQNVRCVI
                     LSRRSRRKIRNTRTRQTSAAQDGRRISEYENLVRAALITTARAGRTRSAKFEILRRPT
                     PPSKYCANIAIRAGRLRQSQDDTNLDWTVNSLRSRRLLFRASSMGKRSGKRMGCEQVG
                     QGEASDDPATIMMCHPSPRFAGPG"
     gene            18781..>19128
                     /locus_tag="VNN25_27605"
     CDS             18781..>19128
                     /locus_tag="VNN25_27605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95373.1"
                     /translation="MIIAQLLLPGASAYERKSQAIDAVSLAAEHDVRVGAVADADIVH
                     IYAPRTLDPAALRDITLPFVSNVRPKASRFSWRKVRQPARVITPLKEPRDLPKTTPNQ
                     EISATEDDETARRR"
BASE COUNT         3910 a         5743 c         5960 g         3515 t
ORIGIN      
        1 ggtgctggtc agctcgattg agatctgcga aattgccgca agaatgtcca cgatcggacg
       61 gctagagacg tcgtatccca gacggaagat aagctcgctt tgtttttcgg ccgtctgccc
      121 gccgagtaaa gctgcggtct cggacgcgca aagcgtccac aacattgcaa gcaggcgttt
      181 aacgctctct ttgggttcgt cacgaagcat ctccagtata gctgttgcag cctgatccgg
      241 agtgcttagc aacttgtcac gcgtctcgat cggccatcct tggcgttcgc aagcgatctc
      301 gacgcgatcg cgtctcacgg cggcggcgat cgctgcggtg ttctcgatga ctagcttgcc
      361 atccgtcctt ccgatatagc cggctagcat ctcatcacac tgacgaatca catcgcgagg
      421 cacgccgtac gagaccgccg cgatcaccgt cagcagttgt tcgccaacgt cggaaaagcc
      481 ccggcgctta caatacgcgc gaaccatctc cttacagtcg ttccagccaa gttgtggcac
      541 tcgcacgatg tgatccagcg aactgtcaat ctcattctta ccgtccgctg atgccagata
      601 gagcccggcc aaagtatcct cagcaagaga aacataataa taaacgccag gtacttcaaa
      661 gatacctttc atcttccgta ggaactcgcc cacagatttt tgctctacga tcttatcaag
      721 ttcatcgatg caaaccatga cgcggcgtcg ggagccaaac gcgccggcat ccacatggcg
      781 gacgacggtc tccgcgaagc gacgatagtt cgacgtcaaa gacatcatgc tagacccgga
      841 ttggtcgccg cggttggacg cggttgcggc caaccgaaag gcgaaagtcg caaccactgc
      901 cgcggcgacg ccgcatccga ttgagaacaa cgcctccgaa gtcgttgacg tgtcctggta
      961 tccccacact ataaagccga ttagggtccc aatcgtaacg ccgagcgcaa tcgccaccgc
     1021 gttgatcaag cccatcggca acgttggagc gcctgcgcga ccccggcgta tgacgaggta
     1081 ttgcagcgcg gtgcgcgcct cgcgataaat caacacaatg ctcgccgttc gcgaccgatc
     1141 gcgttcaagc catggcgaga gatctacagg ttgcacggac gctaaatatc gcacgagcgc
     1201 caaggtggcc atcgcggcaa cgacaagcac gggcatgcac gccatacgga gttccggccg
     1261 cgctttctcg ccgaacgcga acatcaccaa taccccgaat gccgccatgg tcgtcgcgaa
     1321 catccaggtt ccagcagaag cttgcttggt ttctagtaag cgcacttgca aaggcttcgc
     1381 accgagttct ttcccgacag catgctccgt ttgcaatgct aatcgctcaa ataggctttc
     1441 gacaaaatca gcttcctcat agtttgtggg agcggcaacc cacacgctta cagcagggtc
     1501 cgcgggggtt ggctcggaag gtttgtagat ttcattgagt atccgcgttt tgcctatacc
     1561 ccttcttccc actagagcga ttgtgctcca ccctcgaccg ctgagctttc ggacgtggtc
     1621 caggagtggt tcgcgcccga gcagatcaac tgggagtcca atgtcgccgg tcgattctcc
     1681 ggcttgcgtc tcggcgttaa ccaggaggcg cgtccgatcg cgctctacga gcttgcgtgc
     1741 cgtaaatcca ccgaaagcga tagcgattac gctccccggc agcatcagca tcgtcgcgaa
     1801 caaacaagcc agagttggcg atagagtgtt ggcctgcaag gcggcgacgc gaatcggcgc
     1861 gagtgagtcc ttacccgaag cccgataaag catgatcgcg gcgtgcagaa gtagttgctc
     1921 gcgattcgcc ggcgtgagag cccggaacgt ttcgaatttc tctgatagcg agagatacga
     1981 cggtgtcttc aggattacgc gcatagctgg agtcactggg tcggaaatga tttgcagaaa
     2041 ctctttcgaa ttccgcaggg cgtgagtaga cgtgacaaag tcgaggacac cgggagaaaa
     2101 gagcggcgca attgaaatat cgttgcggtc gctgtcggag attaatttcg cgagttgcgc
     2161 gcggtcagta tcgttcttct ggaaaatacg cacgagctct ttgcagacag gcacgcttgc
     2221 cccgaggtcg gcgatgcatg ggtcggcctc gttgcggcgg aggtgattgc tgagatccag
     2281 ccttgcggcg gccgatgtgg gaaaaccgtc aagcttaggc tttccgtcgg ccagggattt
     2341 cagaagatcg aaaactggaa ggtcgggtgt tgatcgagca acatattccg aaaacgcgct
     2401 ttgttgttcg ggtgttaacg aatcaaaggc gtcaagttgg ctctcgagct cggtcggccg
     2461 ctctttcact gctagcctga gcgctgctcg gtatgcgagg aacccctcct cgcgttgtgg
     2521 caaggccatc tgcatccgga ggtgacggtt gaaagcgttg tcagtagttt tcgggaaaag
     2581 catgtcggca atccgtgcgc gttcgattgc tcgacgggca tcgggtggcg ctgggaaatt
     2641 gccggatatc atcgttccat cgcgcagtcg aagcgatgcg acgaccgtcg acggatatac
     2701 gaaagcggcg gcatcccacg gaatggcgga cgcggtagga aacgcgtaga gcaacctgta
     2761 ttccgagacg gtcctgtaac ggtcgctcgc cgtaagcccc ggcattgata acgggacctg
     2821 ttttgtagtc gccatcagag gtgtaggatg gatgaacggg agacgtgtcg ctaggtagtc
     2881 ggtcagagaa ggggtatcga gtttgtcgac gtgactctca agggtcttaa gccattcgaa
     2941 tcgcatccgt tttagtgtct cgcgtcccgc agcttgaagg gctgtcgaaa gttggttagg
     3001 cgttgagcgt tggccgtcgt tatctgcgcc gccgacgagt gcgttggcgc catcccctct
     3061 cgcggataca gtcgcattca aggtcgccgt ttgagctgcc gcggcttccg cagctttctt
     3121 ctcaaaggcg ttgatttcag tcacgatttc aaagtattgg cgacggtatt cgtcactgag
     3181 gatgctttcg gatgccaccc gataaaatat cccgcggttc cacaggtcaa gtgagcgcaa
     3241 ctgggccagc gcagcttcag cggaatgttt ctgatcgtct gacccctcaa acttaggcgc
     3301 tttctcatcc gcgatggtat cgttaacgaa gtcgtaaccg aggattatcg cagttgacaa
     3361 ccctagagca ataagtccaa gcgtcacgcc tcgacggcgg gccggtgtca catcgcgtgc
     3421 tagcgggttg cctgatacat acatgagggc tgcggccgtt gcgaaggtta gcgcgagcaa
     3481 cggaagcaat agcggcaacc ctggctccac gccatgtagt tgggtcgctg ttttggagct
     3541 acccggcgca attagaagaa cgatcaatga cgcgagcgac attgcaccag cggccatctc
     3601 gccaattcct ctgcgccatg ccagatagag tccgaatgcg cagcctgcag cgatacatgc
     3661 ggagccaata agcaccatcc cgtcgaaact tggcgcctga cctgaaacga tgatcgaatg
     3721 gcagaggatc gcactcaaga acgcgctgta tgcaaggagg atcgcaagat cgagaagact
     3781 ccgcgacttc ccggtggcac gggggcccaa ggtatcgtcc tttgccgcgg acaggcgccg
     3841 tttcaggaac tcgatcagaa tcggagtcag cgaccccagg acggctacta ccacctggac
     3901 tatctgcttt tcgacatcgg ccatgccccc tcctcgcgtt cgcaggacgt tctaagtctt
     3961 tgttttggaa ggcacgaatt atagacacaa acgcggacgg agcaactcct tgggcaactc
     4021 tgtgcctacc gaacggactg aacctctcag cgcgtagcgg ttcggcccga gtccgtgaag
     4081 cggaggtcgc tggcttagtc ggacgacgct agacgtcgaa gaccgacatc ggtgcggtca
     4141 ctttgcggcc cagagcccat ggcagccgaa tcgccgtttc cacattcggc acgtgtgaac
     4201 ctttctcgat ggcgcttatc cggctctgag gaacaccgca ccgctccgag acttgaaact
     4261 gtgtcaatgc gcgcttcttt cgcagttggc tcacacgttc gccgagcagt tctccgatcg
     4321 ttggagttac tgccatcgct gcgccttcgt cttgtcgagc tgcggacgac ggcgctcggg
     4381 aagtggtttc cgcgggtgga gatgagagca aggggttgga gatctggccg atgcacgtca
     4441 tcgaagaggc gcttggggac gatgcggacg gcattctgcc gcaggcgatc cggagcgcgg
     4501 agctgcacgt ggttgatgaa aagccttatc cgcagatgac gcagatggag agagcgaacg
     4561 gctttcatct gcgctccatc agcgtcatct gtggatcgtt ttctcgcgta acgtaaccgg
     4621 cctgtttcca agtacttgcg caggtatttg cccacgcgtt tgccacagcc atgtaacctc
     4681 gcccgagctc atccgtctaa gggggtgtat ccaaaaaacg gaggcgagtc atgtccggtg
     4741 tgcaacctat cgggtccttc gaagagagtc tgacggatgg cagttccgat cgagtggacc
     4801 tttactggcg ttgggaatct gcggtcgtgg atcggggagc aagcgcgggg atcgacgccg
     4861 atcgagagca ggtcgagatc aaacgatgca ggcgtgagat caagcgctgc aagcgtgcac
     4921 tcgcgcagct cgaccgcgat acgacgcgct tccggctgca cgtcagcgag agcaggcggg
     4981 agatcagccg acgcaggcgt gagatcaagc ggcggcggca tgccagcagg cggcggagca
     5041 agttcattct ccaaggccgg cggcagagga agttccctct ccaacggcgc ctgcttcgtt
     5101 tcgaccagaa ggccgtcgcg ctcgcgcggc gtgatggcag ccgcggccag cgtgatgtca
     5161 gccggcgtct cgatgatctc gtccggattc tgaaagatct cagccggcgc gcgaaagatt
     5221 tcggagcggt tccgaaccct gctccgcgga gagaaagttt cgttccggct agagaaagct
     5281 ttactccagc tagagaaagt ttcgctccgc gtagagaagg tttcgctccg cgtagagaaa
     5341 gtttcgctcc gcgtagagaa agtttcactc cgcgtagaga aggtttcgct ccgcgcagag
     5401 aaagtttcac tccgcgtaga gaaggtttca ctccgcgcag agaaggtttc actccgcgta
     5461 gagaaggttt cactccgcag agagaacgtt tcactccgca gagagaaggt ttcactccgc
     5521 gcagagaagg tttcactccg cgtagagaag gtttcattcc gcgcagagaa agtttcgctc
     5581 cacttagaga aagtctcggt cgagctagaa gaagttttgc tccagctaga gaagattctg
     5641 gtcctgctct gacaggtttt gctccggttg cctccgaaaa aggacgctcg ccacgatgag
     5701 tgaccgccgt gtacggatgc tatgcccctg cggtgcgcgc gcccctcccg ccgccctctc
     5761 gatggcatcg cgcggatggc gtgtcgcctt gccgcgtctg cactctcgtc gtacggcgcc
     5821 gatggtggcg ctgacgactc catgaaccct tttcgaaggg atgcgacagc agcatcctgg
     5881 agtacccgat gtctaccacc acaccgaatc ccggtacccc ggaggcagga acgcctgccc
     5941 ccagtcccca aactctcgac gcgcaggaga ggatcgctcg ccttcgcgtc atcgctgacg
     6001 acttccccga cgaagcgtcg ccgaaaccac tgacgaccgc acagcgccaa ttggcgagcc
     6061 gtacgtcgat cgtcttcctg gagaaggcgg ctctcttcgc cgaggctgct ccgaacattg
     6121 gagtggcgct ttccgtcgat acggcggcat tgcgtgacgc cattgcctcg gagcttgcca
     6181 ccggaggtgt cgtcgacgaa acacggactc tgatctcgcg catggaggac gccaacaccc
     6241 gcaagaagct caaagcggtc aaggttgcgc gtgacgtcta tcgcgtggcc aagagtttcg
     6301 tgaccaccga cctggggcat gttgtgacgc cccatgtcga tgaaatgaag cgcgcattgc
     6361 aacggccgcg cgcgaagaag aaggcggcgc cagcagagcc gccggcaacg acgccggcgg
     6421 taacgcccaa tccgtccgat tcaacgtcgc aacagaagta accgaggggc cggaggagga
     6481 ttgtgattgg ctctcaccct ccccggctcc tcacacgact cgtagttacg agtcgagcag
     6541 tgcccgaccc gcggccacaa accgcgggtc ggattttatt acggaatgcg gcgagagcgt
     6601 ccggcgatct gttacagagc gcgaacgtca gctatcccct gactggaggc ggatgtccgg
     6661 gcggcattga tctctgactc tcgacgggag atatgtcggt atcttccaat cgcccgtcca
     6721 gcgcagaggc acagatcatt acgtcgaggc gagacgcctc gacgccgcgg gcgagacgcc
     6781 cgcactccta tctcaggcgc tcgaagcagc gatcgcacca tgcggcggag tatggtccct
     6841 tcggagcatg gcggagatga ccgggcgcgc cgcagttcac gcagatgccg cagaatcccg
     6901 gttccactcc ctgcgatcgc aacacctcgg gcttcgttgc ccagaggccg cagtagcatc
     6961 cccgcgcgtt gggctcgatc ggcgcgtcac cggcggtcag agcgacctcc tttgctgcag
     7021 tcttcgcagc aatctacgac gcctgaatcg acgatgacga cactctgcgg atcggcagcg
     7081 tattgctgac gagcggcgac ggctcggttt gggcgttctc gctccgagcc tccgcgtcga
     7141 tcggcggatc gatgagatcg tggagcatga ccatcgcttc gagcttggcc gcgatccgct
     7201 gcttcgtctc accatcggcg cgcatcatat cggccagaaa cacgtaggcc gagccgcgcg
     7261 cgttcttgtc ccatcgctgt acgaacgccg tcagttgatc gaatgtgcgg ataccggtgc
     7321 gctccacgaa cgggacgaag tgacgcgcca ggtgcgtgcg cagccgtgcc tcccccaccc
     7381 acgtcttgac ctgcgggtag gagaagccgg tgcggagcgc gatctcgatc ggctcgctgg
     7441 tggccaggtt ctccacgttg tcgactccct cccgctccag acgaaaggcg tggctcccgt
     7501 tcatgccgga cactttgttg agattcgtcg agccgtagcc tggtccttcg gcattgcgca
     7561 acacgcggtt ggccagatgc tgaatcgtct tcagcgcgcg gctcgggaaa tagccgaaga
     7621 agaacccgag cacgggaagc agcgccgcca tctgttcgta cgaccggtga atgcccgtga
     7681 cattgccgac ggaactgacg acgttgaagg cggccttacc cgcggccgcg gccgtatcct
     7741 gctcggcgaa caagatggag agtccgaatg cggcaacgag tgccgtcgtg ctggcgacgg
     7801 tgatccgcac cgcggcggag acgaaggcgc tcggcgagag gtcgacgcag aagtagccgc
     7861 ggatgagctg cgcaatcgaa tagatgtacg cgccgaggaa gccgaacgcg aacgctgtca
     7921 gactgtgggc gaccatgtga aagtggccgt ccttggtgta gttctcgacg tagggcccga
     7981 ggagcagcag attggcgccg cggctgaaat cgagacccgg ccggggcatg tcgccggcgc
     8041 caccgacagg cttgagcagc agcaacacgg ctgctccaaa cgcgacgacg gtcgccatgc
     8101 cggacacggc gaccacgtag tcacgccagt ggaactgctt ggcgatgtac tcctcgatcc
     8161 agaccctgtt cggctcccaa agcgcgccaa gttcccgccg gatgcggcgc gtcttctcct
     8221 ttcccatgga gaacgtgtac gaaatcgcca tgagcgccgg aaagagcacg gcgaagaaga
     8281 gaagaaggag gttgtagccg aagaagggag ggtcgaacca cgttgccacg gtcctcaatg
     8341 ccgcgaccgg tgtgagctgc ggcagcaaga accagacgct gccaaccaac gatgcggcga
     8401 caaccatcaa cagcacgagt gagaacaaac cgacgagcga tgaccggttc atggggcctc
     8461 cgggactgtt tccgtaacga catagtgcga cagagggggc tgtcgttaca agtgacggac
     8521 actcagaggt tcgggacgtg atcagagatc ggagatcaga gagcagagat cagagatagt
     8581 tcccttcctg gtctcttgtc tctgatctct tgtctctgat ctcttccgcc aacccgtcaa
     8641 gtggcggcac cctcgcagcc cgacgtcggc cagggggtgc cgacgcgcga ggatgccgcc
     8701 gcttcacggc cggctgtcaa atggcgacac cctcgagccc ggtgtgcagg ccaggaggcc
     8761 agcacgcgaa gatgccgccg cttcgcagcc ggatgcgcag tccaaatcca aaggagagtt
     8821 gtggcgtgaa tgccactgcg caaaccgatg cggttacgcg cgaggagggg aacgcgggtc
     8881 ggagggtgtg gcgagaagaa ccagcgctgg ctggagcagc cgcggctcca cgcgcgcgaa
     8941 cgcgacgagc cctacgaaca gcgtgaggaa cgccacgatg atggcgagcg cctcttcgag
     9001 cgaattacga tcgagggcga ggtcatccga gacagagata atcgggaaga gcaggacgag
     9061 gatggcggca agggcgagca gcgccttgcc cgagcgccgc ggcacgaaca cgaatgcggc
     9121 caccgccaca gccagccaga cagcgttgaa gacgatctcg atcattagcg tttcgcggca
     9181 gccggcttct ccaccggccg ccgcattgta aggctaagtc tcacggtccg cagacagttt
     9241 cgatcaactt tgcgcaaacc tgatacgggt ccatgttcgc ggcaggccgg cggtcttcga
     9301 agtatccgcg accagcattc gtggtcgcca ttgggatgcg cacggaagcg ccgcggtcgc
     9361 tgacaccgta gcggaactca tgaatcggag cggtttcgtg aagacccgtc agccgctctt
     9421 cgttgtgtgc gccgtagacc ttgatgtgct cgtcgtgacg cttggcgagt ttcttcatcg
     9481 cttcttcgat gaccttgatg ccgccctcgg cgcgcgtttc cttcgtcgag aagttcgtgt
     9541 gcgcgccggc tccgttccag tcgcctttga ccggcttcgg ataaagcgtg gcgctgatgc
     9601 cgaactcctc gccgatgcgg tacagcagcc accgcgccag ccagagctcg tcggcgatgt
     9661 caggcgcctg gaccgctccc acctggaact cccactgcgc cggcatgacc tcggcgttcg
     9721 ttccgacgat gtggattccg gcgcggatgc aggcgtccgc atgcgcttcg acaagatcgc
     9781 ggccgaagac ctcgtccgct ccgacgccgc agtagtagcc gccctgcggc gcggggaagc
     9841 cgctggcggg ccagccgagg ggacgattcc cttcgaagag cgtgtactcc tgctcgatcc
     9901 cgaaccagca gtcatgctct ttgtagcgct cggccacttc gcgcagaaac gcgcgcgtgt
     9961 tgctcggatg aactgtgccg tcaggattga gcacctcgca gagaacgagc acgtcgccgt
    10021 cgcggtgcag cggatcgggc atcatcttca ccggccgcag aagaaggtcg ctgaagtgcc
    10081 cggtagcctg ctcggtgctc gacccgtcgt agccccattc cgggatgtcg gtgagcgact
    10141 tcaccgagtc gatgatcttc gttttcgaac gcagcttagc cgtcggtttt tgcccatcga
    10201 tccaaatgta ttccgccgtg atttttgcca aggaactgac ctcctgcttg agatgtcaca
    10261 agatagcaga acggcccgcg ctcaaaaacg gctgccgttc agatccggcc tgttcaggga
    10321 acgatgaggc gatctgctag acgcggctcg cctcggacgc gctttgcaac tcctggccca
    10381 ccctgctcac cacgacccga ttctttccga gtgatttggc ttgatacatg gcggaatcgg
    10441 cgcgccggag caggtcggtc tgctccagct ccagcgaccg gttgggatcg gtgtcgggga
    10501 catgttgcgc gatgcccagt gacgccgaca gcagcccgcg cagccgcaac ggctgcatac
    10561 tgaaacccca ctcccggtcg agaaagacgg tctgctcgat cgtctgccgg atctcctcgc
    10621 agaccgccat cccctcttcg agcgtggtgt tcggaagaag gatgacgaac tcgtctccgc
    10681 cgtagcgcgc gacggtcgct tcctcctgcg gaacgatgcg cttcaggaga aaaccgacct
    10741 cgcgcaatac ctggctgccg gccagatggc cgtgagaatc gttgatggct ttgaagcggt
    10801 cgaggtccat gaagatcagc accccttcgc ggccgctctt ccgcgcctcg gcgatgctgt
    10861 cgtgcaagcg gacgttgaac cagcggtcgt tgtaaaggcc ggtgagatcg tctttctttg
    10921 cgagctcgtg ggcgcgcttg gcgtcgagcg cgttctgcaa ggtgaaggac gtgtagccgg
    10981 cgaagatctc cagcagcgtt ttgtcgcgct ccgtgaagat gctcccctcc agccggttga
    11041 tcaactccag tacgccgcaa acggttttgc cgatatagat cggcacgcag acgacggagt
    11101 gggtcttgtg gccgagctgt tgatcgaact ccgagtagaa gaagtcgtct tccttgacgt
    11161 cggcggagag atagggcgtc ccggtctggt agacctgccc gacgatgccg aacttcgcgg
    11221 agagccgctg accgatgaga tcgcggctgg atcccccgaa ggtggcgatg aagacgagct
    11281 cgttctcgcg gcgcaccgca accttccgga agggatcatc gaggaggatc gagcccgact
    11341 ccgaggggac gaactcgttg gccttctgca ggatctgtcg aaggatgaga tcgagatcga
    11401 cgtcggactt gcgggaaagc cccgctttct ggcgctccag gaagcgttcc aactgcggaa
    11461 tctcgagcgc gtgaaattga atcatggtgt cgtcgtgatg gagcgattat acgagttgct
    11521 tccctcagcg gcgaacgcat cgacctcgaa agagatccca cctcatccac cggcgtcccc
    11581 tacaatctgt ttcatgcgaa aactgttcgt tgtcatcgcc gcgctcagcg ccacatcgct
    11641 ctttgcccag caggatttca gcaaggtcga gatcaaggtt acaaaggttg ccggcacggt
    11701 gtacatgctc gccggcgcag gcggcaacat cggtgtgtcg gtgggcgacg acggcatcgt
    11761 catcgtcgac gacgagttcg cgccgctcgc gccgaagatc cgggaagcgc tgcacgggat
    11821 cacggccacg ccgatcaagt tcatcctgaa cacgcactat cacggcgatc acaccggcgg
    11881 caacgaagtg ttcggtcacg acggcccgat catcgctcac gagaacgtgc gaaagcgcct
    11941 cgcatccggc tcgaaggcag ccgggcgcga gacccctccc gcgccgaagg acgcgttgcc
    12001 ggtgatcacg ttcaacgacc gcgccacgct gcatgtgaac ggcgaggaca tccgcgcggt
    12061 ccacatgcct cacggccaca ccgatggcga cgcggtcatt tacttcacgc agtcgaacgt
    12121 cgtccacatg ggcgacgact tcttcaacgg tatgttccct ttcgtcgacg tcgataacgg
    12181 cggcagcgtg cgcggcatgc tggccagcgt cgacaacgtg ctgaagacgc tgcccgatga
    12241 cgcgaaagtc atccccggcc acggtccgct gtcggacaaa gcaggcctcc gcgccttcgc
    12301 cgaggttctg cgcggcacga gcaacgcagt cgccgcagca ctggcagcgg gcaaatcact
    12361 cgatcagatg aagcaggaga acattctcgc tccgtgggat agctggggcc aaggcttcat
    12421 caagagcgat gcatggctgg cactgctggt gtcggaggcg aagaagcagt aggtttcgtg
    12481 ccgctataat tcttcagcgt ggaagacaag agtatgttgc acggctccat gaaactacgc
    12541 gatcagctga cagaaatggg gcggcattcc gccgagctcg cggctgtcct aggagtcgtt
    12601 ttcctcaacg tggttgcgaa cggaacgcga acacgctcct ggtctttctc gcttttaatt
    12661 gccttgtctc tagtcggagt ggccattctc ttcgtgatca ccttcgcgat catcattcgg
    12721 ggattccgag atcaacctgc gggcgtgcgt gagatgcgcg attcactcgt ttcatcatat
    12781 ttggctgcgt tagaccgaac atcgttgaac cccgcaacgc agccgcacac tcagatatct
    12841 gtccggcgac acctcgatgg agacgacgag gggaagcagt gagttcggac aattccggat
    12901 ccagtttttc cgaccgcttc cttttcgttg gagtgctttc caatcgagcg ccttctcccc
    12961 ccgcgctttt tgcgggggga gaaggtggcc gaaggacgga tgagggggga gcagatacgc
    13021 agcgcgatca accacgcctc gcccgaacag cctgaaaacg cggcacatcc acgcgtatcg
    13081 tgctcgatgg accgccccct catccggctt cgccaccttc tcccccacaa aaagacgtgg
    13141 gggagaaggc gctcgattgg aaaggttgcg ataagagcgt gaaaagcggc caggtctcac
    13201 gctctcaatc gcgcgcgatg caccacctcg gcggtcgcga gcaggacgat cgcgatcgcg
    13261 gcgtacatcg ccgttacgcg catcgccggg gtcgagaaga agccggcgaa gtcgaaggcg
    13321 ggggtctggc cgtggtcgtt cacgacgacg taccagaacg tcaggaatcc gacgatgaag
    13381 gtctttggat tgccgctgat gatgccgagg gcggtggcgg aggcggcgac gaaggcgatt
    13441 ccgatcagca gcgccgggag gcggatgggg tgcgcgatcg caatcttcac caccggcgcg
    13501 atgagcagca gcgtggccac gagcacggat gatgtgagct tccacgttac gaagcgctcg
    13561 cgcagacggg gcgcggcata gatgaggcct gtggttcccg cgcggcgatc gcgtgaggcc
    13621 acatcggcga tgaagatagc cacgatcccg aaggtgatcg ggagcgagtc cggtgccgcg
    13681 agcgacgcga tgctgatacc gatcagcgcg aagatggtga acggcgtcga ggagaaggtc
    13741 atcatcgcgt ccttcagcgc cgcgccgcgg acagggatcg cggcgagtgg acgtccgaac
    13801 ggacgtgcca gcgcgttgaa attgcgcatc catccgcgtt tacctttggc gccggcagcg
    13861 cgcaggcggg ccggatcgaa gcggtgaaag aagagttgcg cgaacactag cagcggaatt
    13921 ggcgtcaccg tcgagacgat gcgcgtggcc caggcaccgc cgccgaggtt gagtccggcg
    13981 atcgtgaccg gcggcatcgc cctattgaaa ctagacgagc cgatcgacat cgatgtggtg
    14041 tgaaagttgt gccgaatctg ctcgaagagg taaccgaaac tgctgaagtc gaagtagcga
    14101 agaacgccgc cgcctccgtg ctccatggaa ctggccacga tgccgagcga tccgcagtag
    14161 aaaaagaagt agagaacgtc gccgatgcgg ccggagagcc agggaatcga ctcgaagacg
    14221 atcgcgacaa cggagacgaa cacgatcgtc gacggcgtga cgatcgcgta ttgcttcaag
    14281 aagaggagcg gctcgagggg cgcctcgccg cggacgacct gcatggccat cgacacgagc
    14341 atgaagccga agacgaaggt ggagaggaag acgacgttgc cgagaaactt gccgaggacg
    14401 tactcgctcg tccgcatgtt cgtcgaggca atcacgtacc cacagcgcga gtcgacgtcg
    14461 cggcgaacgg cgttggagat gacgtaaaac ccgaacaggc cgataaacat cgacgccagc
    14521 atcgacgtgc ccatcccgat tgctgcggag ttgtagagaa cccgcgcacc gtggatctgc
    14581 atcagcgttc ttccggtcga cgtggcggga acccagacgt acgcaaagcc gctcagcagc
    14641 aggaagacga tcaccgtcga agcgcgccgg aagcggatca gaaagtccgc gcgaacgatg
    14701 gctgcaatgc ggctgatcac gctgccgcct tctcccgcat cgtgtacaga aaggcatctt
    14761 cgagcgtcgg ttcggcggca gtcgcgccgc cccgcggctc atcgccgctg acgatgcgaa
    14821 cgtgaacgcc gtccgacttg cgcacggcgc tggaaacgcg cgcggtgcgc cgcagctcgt
    14881 cgaatcgctc cgacggcacc accagctccc aaacactccc ctcggctcct ctcatcaatt
    14941 cctcgggtgt ggcgcaggcg acgatcgagc cgccgttcat gatggcgatg tgcgtggcca
    15001 ccgactcgac atcggagacg atgtgcgtcg agaggatgac gagcttgccg agaccggcgt
    15061 cggcgagcac attccggaag cggacgcgct cttcgggatc gaggcccgca gtcggctcgt
    15121 caacgatgac gagatcggga tcgttgacca gcgcctgcgc gatgcccagc cgttgcttca
    15181 taccgccgga gaatccgccg accggccgct tggcgacgtt gtgcaggttc accatctcca
    15241 gcatctcgtt gatgcgcgcg cggctgtgga cgcccttcag cgcggcgaag tagctgagaa
    15301 actcgtgcgc ggtgaggttg tcgtaaacgc cgaagtcctg cgggagatag ccgagccgcc
    15361 ggcgaatctc gttggggttg cgggcgacgt cgacgccgtc gaactgaatc gttcccgcgg
    15421 tcggacgcgt gatcgtggcg atcatctgca tcaacgtcgt cttgcccgcg ccgttggggc
    15481 cgaggaggcc gaggacgccg ctgccgatcg aaagggagag atcgcggacg ccgtagttgc
    15541 cgccgcggaa ccgtttactg acgccttcga gatgaagcat gcgcacctat acggagcgcg
    15601 agcactgcgg ttatcaggag cgcgagcgtg atcaggctga tcatcctgcc ggtgacgaac
    15661 gcctgcggaa gatagacgac gcgaaccttg tgtcgaccgg cgggcacgaa gacgccgaga
    15721 aatgcgtgat tggcgcggac gattttcacg cgcttaccgt caacgtatgc acgccaacct
    15781 ctccacgcgg tctccgagat gacgacgaag ccatcgcgag tcatcacggc atcgagatca
    15841 aagccgagct tgcgcgggtg gatggcgacg ctgccgggac cgttcgccaa gtcgcgcgcg
    15901 gcgccgggaa gatcgagcca ggccagctcg gagaagtcgg ttgcggcaac catatcctcg
    15961 acgtcgttag cgactcccgc tcggatgttg cgcggcacaa acgcgcgcgg cagaacacgc
    16021 atgttttcga cgaggcgtga gccgtgatcg gcgacgacgc tgcaccagcc gtcggggatg
    16081 ggatcgccgt ccggcacgac tgcgaagcga acgttcagca tcgaaagcat cggacgcgtc
    16141 aaatcgtcga tctgattgaa tccgaaccct ccgcggcggc accacgcggg aaatgtctcg
    16201 tcgtacgacg tgagcgtcat cgccgtcagg acgcgggcat cctcgagacc gtacatcgtg
    16261 gcagtgttgg ggaagagtcc tggtccggtg gcaacgatgc ggaatcgctc atgaatgttc
    16321 tgcaacggct gaaagagcgc gagttgcgga taggcgatgc gcgcgtcgtt agcaggaaca
    16381 agcgcgccat ccgagatcgt ccgctgcagg aggatcaatg cgaagaggat cggaatggca
    16441 atcttccggt tcgccacgac cagcaccacg attgccgcca gcgccagcgg caccacctcg
    16501 gccacgaaac gcggcatatc gatcgatccg gcacgcatcg ccgaagctgg tacggcaaga
    16561 gccacgagca gtcccaccat cacgattccg gcgcgatccg aaagggcatc gatcgcgagc
    16621 gcggccagga tcgccaggca aagcgggacg gccgaggcga gccggtcgtt cagtgcgaga
    16681 ttgaagaggg gcaatcggtg aaggagctgc gcgaccggcc aggcattggc gccagcgaga
    16741 agggtgagaa agccgagtgt cgcgaagaac catcgctctc tacctcggcc tcgccacagc
    16801 gcgaagatcg ccagcgccag tgcgatgctt cccgcttccg ctcgcgccat cgggaaattg
    16861 cgaaaagagt tccttgcgaa gggcagcaaa tccgacaaca acgccgcgcg cggaaggccg
    16921 gcggcaacgc gcaatgtgcc gggcgcgaag aactcggtgc ggatgcgatg ctcgaccgtt
    16981 tggttgctcg cgtcgagcat cggcaggatg gcgatggcgc tgagcagcag cgcgatgatg
    17041 ccggcaccga ctgcgtaccc gaacgcgcgg acgcgctgtt tgccatcgct ccgcgccaac
    17101 tcgaagacgc cgtacgctgc gccgattgcg gtgacgtgga ggagggtctc gggatgacca
    17161 gccagcgcct cgagagtgag cgcaaccaca agaatcacta cggatcggat cgaaggcaca
    17221 gcgacgacgc gtcgcgtggc cagcagaatg agcggaaaca gcgcccagca gaatccaagc
    17281 ggccagagaa tctgcagcgc gatcggcgca ccgaacgcaa agaccgccgc ggcaacgagc
    17341 gacgccatct cgctgcactc caggtcgcgc gcgagcagaa acgcaccgag cgcggcgagg
    17401 aagaaggcca tcgaagccgt gaagccgaag ctcgccgcga cgggcagaat gagcgcaatc
    17461 agtgtgaatg gactgaacgg cgccggctgc gcctcccccg ccagaatgtc gccacatccc
    17521 tcgaaccgat tgagaagcgg ccactcgccg tggccgatgg aatggcgcac cgcctgccgc
    17581 cacggaatca tctgaaaggc gatgtcggac agcatccgat tctgcggatc gggcaccgca
    17641 agctcgctct gatgcgcata caacggctca ctcgaatacg tcagctcgac cggcgcgtaa
    17701 acgcgccccg ttagcaggcc ccgtccggtg aaaaccaatg gcagcagaag aaggacgagc
    17761 gccacgccgc gcgagaggcg cgtgatgtac ctatgcgcga gagcgagcac cgcgatggcg
    17821 gtcgcgaggt agagagcgag tgtcatcctg ggccggcgaa gcgcggcgaa ggatgacaca
    17881 tcatgatcgt cgccggatca tcgctcgcct caccctgccc cacttgctcg caccccatcc
    17941 tctttccgga acgctttccc atcgaggagg cgcggaaaag gagccttctt gaccgcaatg
    18001 agttgacggt ccaatcgaga ttggtgtcat cctgagactg ccggagccgc cccgcgcgaa
    18061 tcgcgatgtt tgcgcagtat ttgctcggcg gcgtaggacg tcgaaggatc tcaaacttcg
    18121 cagatcgtgt tcgtccagcg cgagcggtcg tgatcaaagc ggctcgaacg agattttcgt
    18181 attctgagat ccttcgaccg tcctgcgccg ccgatgtctg gcgggtgcgc gtgttgcgaa
    18241 tcttgcggcg gcttcggcgg ctcaggatga cacacctcac gttttgcgcg tatcttgggc
    18301 cagcgggctg gatgccagcg agaagtctgc aaactgtcgc tgcagatgga agagccagac
    18361 cgacaagcgg gcgaatacca aggcaacacg ccgagctgtc gcgacaaact gccgtgctct
    18421 cgcgacaaac tggcgtgcag tcgcgcttgt gcgccatgca gtcgcgtttg tcaggcgtac
    18481 tctcgcgttc atctgccatg tttgccgcgt tcaggtggcc agctgtcgcg ttcatgcggc
    18541 ttgctgtcgc gttcatgggt cgtgttctcg cgttcacatg gcgagttgtc gcgttcacgc
    18601 ggcgtgatgt cgcgtttttc cgactgaaat cgttgttcat ccacagtgtt gccgcgtttt
    18661 tctgctcaag agtcgggagc gccttctttt cccgcagatg acgcagacag acgcagatgt
    18721 ttccatctgc gttcatctgc gtcatctgcg gatcagtaat aacccgttta cgattaccac
    18781 gtgatcattg cccaactcct cctccccggc gcctccgcct acgagcgcaa atcgcaggcg
    18841 atcgacgccg tctctctggc ggcggagcac gatgtgcgcg ttggcgccgt cgcggatgcg
    18901 gatatcgtgc acatctacgc gcctcgaacg ctcgatcctg ccgcgctgcg ggacattacc
    18961 ctccccttcg tcagcaacgt ccggccgaag gcgtcccgct tctcctggcg caaggttcga
    19021 cagcccgcac gcgtcatcac gcccctcaaa gagcctcgtg atctcccgaa aaccacgccc
    19081 aaccaggaga tttcggcaac tgaagacgac gagaccgcga gacggcga
//
LOCUS       DATLVW010000931         3571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_450726, whole genome shotgun sequence.
ACCESSION   DATLVW010000931 DATLVW010000000
VERSION     DATLVW010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450726"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VNN25_27610"
     CDS             complement(<1..923)
                     /locus_tag="VNN25_27610"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HXH95374.1"
                     /translation="MTRCLSFAALLVLSVLALPARGQEPEHGDILGPLPVRDQYLLSN
                     GFFFFEPESARVLDDDDWLVDVHGADSNTFAKSRWISHNLAGDTDRRTGAQTLSLIRM
                     DDGQAVFLVDGEIHRVTITAHRGLGANLEVGIAVPFATIGGGTSDAFIEHFHATFHLG
                     GDERPAMQRNRETIYIRTDDGRTFFRQHTAGAQLGDVALSAKYELPALEDPNLALSVE
                     GALELPTGNAQTLDGSGSLDGGVQLLATRDYGRTRLHASAGMLLLGHNGALGTKSQFV
                     ITDSIAISSLIAGRTSATAQITIAQSPFRTIG"
     gene            1009..1266
                     /locus_tag="VNN25_27615"
     CDS             1009..1266
                     /locus_tag="VNN25_27615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95375.1"
                     /translation="MSRGFVATLGGIALTLLAWFGSWVWPGWPASFALDALGHYSAFP
                     DLPRAAKVAVVVGLIVINVGTWAVVIRGLMLLLRRSFFIRR"
     gene            1336..2334
                     /locus_tag="VNN25_27620"
     CDS             1336..2334
                     /locus_tag="VNN25_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HXH95376.1"
                     /translation="MDFRSDNTHGCSPEIVEALVRANRGTMTSYGDDAVTARLRDRCC
                     ELFGCDVDVFPVLTGTAANALSIASMTTPASRIVCHSEAHIHLEELGAAEFLSGGAKL
                     VPIDGVEGKLSDKDVAAVENFDCLSITQATEAGTLYRPDDLAALGGLAHARGAGVHMD
                     GARFANAVAALGCAPADLTRRVGVDVLSFGATKNGAMGAEMIVVFRKDLAARLSTLWH
                     RSGHRLSKSRFLSAQLEAYLTDDLWLRNARHANAAAARLAEGIRDHVEILQPGEVNVL
                     FVRMEDSLAAALRAQGFSFYDWELFGAGSKRLVTAFDSTDSEIGALIAAIQKSSRP"
     gene            2364..2648
                     /locus_tag="VNN25_27625"
     CDS             2364..2648
                     /locus_tag="VNN25_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95377.1"
                     /translation="MATVMLMHWPEVTKEKYEEVRRDVNWEGEPPAGAKFHTAWMAGD
                     GFHVLDLWDSPKDFETFVQQRLMPSVQKVGVDGQPKVEIFESLSVFAPNV"
     gene            complement(2749..>3571)
                     /locus_tag="VNN25_27630"
     CDS             complement(2749..>3571)
                     /locus_tag="VNN25_27630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH95378.1"
                     /translation="RLRLEAGAILDRRGRLRGRFRGSRKQDEIGDLSRALERLTRRLD
                     EHVRFIESFAADVSHEFKNPLASIRTATEMLAEVDEPDERGRFLRMVEQEIARMESLL
                     SGVREITHIDAQLTVEQRTRVDVHALLERIVDGFRMRERSNVSIVFEAPGQPMEVSAS
                     EDRLIQVFVNVLENAISFSPPAGVVTITLARLGNEILITISDQGAGIPEANMARVFDR
                     FFTHRPDPNRPRASHTGLGLSIVKAIVDGYGGSIAPHNNEAGGATFEIRLPGLTG"
BASE COUNT          569 a         1004 c         1296 g          702 t
ORIGIN      
        1 ccgatcgtgc ggaaggggct ctgggcgatg gtgatctgtg ccgttgccga tgtgcggccc
       61 gcgatcaggc tggagatggc gatcgaatcg gtgatgacga attgcgactt cgtgccgagg
      121 gcgccgttgt ggccgaggag gagcatgccg gccgaggcgt ggaggcgcgt gcggccgtaa
      181 tcgcgtgtgg ccaggagttg cacgcctccg tcgagcgagc ccgagccgtc gagcgtctgc
      241 gcgttgccgg tgggaagctc gagcgcgccc tcgacggaga gcgcgaggtt gggatcttcg
      301 agtgcgggga gctcgtactt cgcggagagg gcgacgtcgc cgagttgtgc gccggccgtg
      361 tgctggcgga agaaggttcg gccgtcatcg gtgcgaatgt agatggtctc gcggttgcgc
      421 tgcatggctg ggcgctcgtc gccgccgagg tggaaggtgg cgtggaagtg ctcgatgaag
      481 gcatccgagg tgccgccgcc gatggtcgcg aacggcacgg cgatgccgac ttcgagattc
      541 gcgccgaggc cgcggtgcgc tgtgatcgtg acgcggtgaa tctcgccgtc gacgagaaag
      601 acggcttgcc cgtcgtccat ccgaatcagg ctgagagtct gcgcgccggt tcggcggtcg
      661 gtatcgccgg cgaggttgtg gctgatccag cgcgatttgg cgaaggtgtt ggagtcggcg
      721 ccgtggacgt ccacgagcca gtcgtcatcg tcgagaacgc gcgcgctctc cggctcgaag
      781 aagaaaaagc cgttgctgag caggtactga tcgcgcacgg gcagcggccc gaggatgtcg
      841 ccgtgctccg gttcctggcc gcgtgcaggc agagccagta cggaaagtac caatagcgcc
      901 gcgaagctca ggcagcgagt catgtcggga atccggttgt gaaaacgatg acgcgggagg
      961 attggatttg tccagtgttt ctttcggccg cgcggataga ctgcctgcat gagccgcgga
     1021 ttcgtagcca cgctgggcgg tatcgcgctg acgctgctgg cgtggttcgg ctcgtgggtc
     1081 tggccgggct ggccggcgag cttcgcgctg gacgcgctcg gccactactc ggcgtttccc
     1141 gatttgccgc gcgcggcgaa ggttgcggtg gtggttgggc tgatcgtgat caatgtgggg
     1201 acgtgggcgg tagttatccg tgggctgatg ctacttctgc gacggagttt ttttatccgc
     1261 agatgacgca gatgagacgc agatacatct gcgtttcatc tgcgtcatct gcggatcaaa
     1321 ctatgattcg tggccatgga ttttcgatcg gacaacacgc atggctgctc gccggagatc
     1381 gtcgaggcgc tcgtgcgcgc gaatcgcggc acgatgacgt catatggcga tgacgcggtc
     1441 acggcgcggt tgcgcgatcg atgctgcgag ctgttcgggt gcgatgtcga cgtgtttccg
     1501 gtcctgaccg gcaccgcggc gaacgccctt tcaatcgcct cgatgacgac gccggcttcg
     1561 cggatcgtct gccacagcga ggcgcacatc catctggagg agttgggcgc cgcggagttc
     1621 ttgagcggcg gcgcgaaact ggtgccgatc gacggcgtcg aggggaagct gagcgacaag
     1681 gatgtcgctg cggtcgagaa tttcgactgt ctgagcatca cgcaggcgac agaagcgggg
     1741 acgctgtatc gccccgacga ccttgctgcg ctcggcggtc tcgcgcacgc gcgcggggcg
     1801 ggcgtacaca tggacggtgc gcgtttcgcg aatgcggttg ccgcgcttgg ttgcgcgccg
     1861 gcggatctga cgcggcgtgt gggcgtggac gtgctctcgt ttggcgcgac gaagaacggt
     1921 gcgatgggcg cggagatgat cgtcgtcttc cgcaaagatc tcgcggctcg tctaagtacg
     1981 ctctggcatc gcagcgggca tcggttgtcg aagtcgcgct ttctctcggc gcagctcgag
     2041 gcctatctca cggacgatct ctggcttcgc aatgcccgtc atgcgaatgc ggcggcggcc
     2101 cggctcgcgg agggcattcg cgatcatgtc gagatcctgc agccgggcga ggtgaacgtc
     2161 ctcttcgttc ggatggaaga ctcgctcgcc gctgcgttgc gggcgcaggg cttctcgttc
     2221 tacgactggg agctcttcgg cgcgggctcc aagcggctgg tcactgcatt cgattcgacg
     2281 gattcggaga tcggtgcgct catcgcggcg atccagaaat ccagccggcc ctgacgatcg
     2341 taattgttgc tcggagggtt cacatggcaa ctgtgatgct tatgcattgg cccgaggtga
     2401 cgaaagagaa gtacgaggaa gtgcggcgcg atgtgaattg ggaaggggag ccgccggccg
     2461 gcgcgaagtt tcatacggcg tggatggcgg gcgatgggtt ccacgttctc gatctgtggg
     2521 actcgccgaa ggatttcgag acgttcgtgc agcagcgttt gatgccgagc gtgcagaagg
     2581 tgggcgtcga cgggcagccg aaggtggaga tcttcgagag tctgtcggtt ttcgcgccga
     2641 acgtgtagct ttctaaaacc gatcaccaca gagacacaga ggtcaccgag aggcagttca
     2701 tgacctctgt gttctctgtg ttctctgtgt ctctgtggtg atcggttctc atcccgttaa
     2761 gccgggcagg cggatctcga acgtggctcc gcctgcctcg ttgttgtgtg gcgcgatcga
     2821 gccgccgtag ccgtcgacga tcgctttgac gatcgatagg ccgaggcctg tgtgggatgc
     2881 gcgcgggcga ttcgggtcgg ggcgatgcgt gaagaagcga tcgaacacgc gcgccatgtt
     2941 cgcctcgggg atgcccgctc cctggtcgga gatcgtgatc agaatctcgt tgccgaggcg
     3001 ggcgagggtg atggtgacga cgccggcggg cggggagaag ctgatcgcgt tctcgagcac
     3061 gttcacgaat acctgaatca gccgatcctc ggacgcactg acttccatcg gctgcccggg
     3121 cgcttcgaag acgatcgata cgttcgagcg ctcgcgcatg cggaagccgt cgacgatccg
     3181 ctcgagcagc gcgtgaacgt cgacgcgcgt tcgctgctcg acggtgagtt gggcgtcgat
     3241 gtgcgtgatc tcgcgcacgc cggagaggag cgattccatc cgcgcgatct cctgctcgac
     3301 catccgcagg aaccgtcccc gttcgtccgg ctcatcgacc tcggccagca tttccgtcgc
     3361 cgtgcggatg gaggccagcg ggttcttgaa ctcgtgcgag acgtcggcgg cgaacgattc
     3421 gatgaagcgg acgtgctcgt cgagacgtcg cgtgagccgc tccagcgcgc gggagaggtc
     3481 gccgatctca tcctgtttgc gcgaaccgcg gaatcgtcct cgcaaccggc cccggcgatc
     3541 gagaatcgcg cccgcttcga gccgcaaccg g
//
LOCUS       DATLVW010000932         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_38967, whole genome shotgun sequence.
ACCESSION   DATLVW010000932 DATLVW010000000
VERSION     DATLVW010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38967"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..580
                     /locus_tag="VNN25_27635"
     CDS             236..580
                     /locus_tag="VNN25_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXH95379.1"
                     /translation="MAVRKGDLLQGTLDLLVLRVLGAGPNHGYGITARLHQLSDDVLR
                     VEEGSLYPALYRMEEQGLIEAEWRPTSNNRNAKFYALTRKGRRAAQAELDGWLRLSGA
                     VARVLRALEQET"
     gene            654..>2103
                     /locus_tag="VNN25_27640"
     CDS             654..>2103
                     /locus_tag="VNN25_27640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH95380.1"
                     /translation="MEREMAFHLDSLARDYARDGLSDVDAQRAARRKFGNLTRLKEHG
                     HDELTMRLVEDVTRDVRHAARGLWRSPGFSLAVILTLALGIGGNTAVFSVVDQLLLRP
                     LPYPDGDQLVVVEETVGANPHADVSPANWLDWQRESRTFRRFAAWRSWSFTLTGTGEP
                     RRVNAQQVSSEFFPLLGVAPLLGRTISDEDDRPNGPRVAVLSYRAWQNELGGDPRAIG
                     RTVQLDDRAYEIVGVMPPGFRFVQQDVDLWTASQLDRTRPWRETEGRVIDVVGRLAAD
                     ATIGTARSEMEGIARRLAAMYTFNKNTSVTVTPLREVLTGQVRTSVLVLFAGVGVMLA
                     IACFNVANMLLARAASRQREIAIRASLGAGRWAIARSVLVESLLLAGAGGALGLALAR
                     GSLDALLAVAPTNLLGVSELFIDRRVLIYAFGLSLATGAVAGLVPTILFARRSMADAL
                     RMRGSKAGHAPRVRQALVVVQVAMTVVMLSGAG"
BASE COUNT          335 a          684 c          703 g          381 t
ORIGIN      
        1 cgaccccatt ggcggggact gccggtattt agtacatact atatagcgct tcagtaaagt
       61 cgccaatata caatgcgagt gtggattctc aagcaaggta gagcccgtga ccaatgcgca
      121 ccccccaaac ggagcgcgta cgtctggtct tcccttgacc atctaagtaa cgctgtgttc
      181 tactgacgct cactcagacg ctctaagtga ggtgtgctgg gaaggagtcg gatcgatggc
      241 cgtacggaaa ggcgatctgc tgcagggcac gttggatctc ctcgtgctga gagtgctggg
      301 ggccggaccg aatcacggct acggcatcac ggcgcggctg catcagctct ccgatgatgt
      361 gctgcgcgtg gaggaggggt cgctctatcc ggcgctctat cgcatggaag aacagggcct
      421 gatcgaggca gaatggcgcc ccactagcaa caatcgcaac gccaagttct acgcgctgac
      481 gcggaaggga cgccgcgcgg cgcaggccga gctcgacggc tggctgcggc tgtccggcgc
      541 cgtcgcgcgc gtgctgcgcg cgctcgagca ggagacctga catggccctg cgacctccgc
      601 tctttattcg tcgcgtactc gcattgttcc gatggcgctc gagggatcgc gacatggagc
      661 gcgagatggc gttccatctc gattcgctcg cacgcgacta cgctcgcgac gggctaagcg
      721 acgtcgacgc gcaacgcgcg gcccggcgga agttcggcaa tctgacgcga ctcaaggaac
      781 acgggcacga cgagctgacg atgcgcctgg tagaggatgt cacgcgggac gtcagacacg
      841 ccgctcgtgg gctctggcgc agcccgggct tttcgctcgc cgtgatcctt acgctggccc
      901 tcggcatcgg cggcaacact gctgtcttct ccgtcgtcga tcagctcctg ctgcggccgc
      961 tgccgtaccc agacggcgac caactcgtgg tggtcgaaga gacggttggc gccaacccgc
     1021 acgccgacgt ctcgcctgcc aactggctcg actggcagcg cgagagccgc acgtttcgac
     1081 gattcgcggc ctggcggtcg tggtcgttca cgctgaccgg caccggcgag ccgcggcgcg
     1141 tgaacgcgca acaggtgtca tcggaatttt ttcccctgct cggcgttgca ccgctcctcg
     1201 gtcgcacgat ctccgatgag gacgaccgtc cgaacggccc acgcgtcgcc gtactcagtt
     1261 accgcgcctg gcagaacgag cttggtggtg atccgcgcgc catcggccgc acggtccagc
     1321 tcgatgatcg cgcgtacgaa atcgtcggcg tgatgccgcc cggctttcgc ttcgtgcagc
     1381 aggatgtgga cctgtggacc gcatcgcagc tcgatcgaac ccggccgtgg cgggagaccg
     1441 agggccgtgt catcgatgtc gtcggacggc ttgctgccga cgcgacgatc ggcacggccc
     1501 gatcggagat ggagggcatc gcgcggcgtc tcgccgcgat gtacacgttc aacaagaaca
     1561 cgagcgtgac agtgacgcct ctacgcgagg tgctcacggg ccaggtgcgg acgtcggtgc
     1621 tcgtgctctt cgccggcgtg ggtgtgatgc tcgccattgc atgcttcaac gtcgccaaca
     1681 tgctcctggc gcgagcggcg tcgcgccagc gcgaaatcgc gattcgcgcg tcactcgggg
     1741 cgggacgatg ggcgattgcg cgatcggtgc tggtagaaag cctgctgctc gccggcgccg
     1801 gcggcgcgct cggcctcgcg ctcgcgcgcg ggagtctcga cgcgctgctc gccgtggccc
     1861 ccacgaacct gctcggcgtg tccgagctgt tcatcgatcg acgcgtccta atctatgcgt
     1921 tcggcctgtc gctcgcgacg ggcgcggtcg ctggcctcgt accgacgatc ctgttcgcgc
     1981 ggcgatcgat ggccgacgcg ttgcgaatgc gcggctcgaa ggccggtcac gcgccccgtg
     2041 tgcgacaggc gctcgtcgtc gttcaagtcg ccatgaccgt cgtcatgctc tccggcgccg
     2101 gtg
//
LOCUS       DATLVW010000933         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_617230, whole genome shotgun sequence.
ACCESSION   DATLVW010000933 DATLVW010000000
VERSION     DATLVW010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617230"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VNN25_27645"
     CDS             complement(<1..897)
                     /locus_tag="VNN25_27645"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXH95381.1"
                     /translation="MTAFGDIDVLEFGEVPTPAPRPGHVLIRVEAVGTNYYDTLVRSG
                     AVSRLIPLPHVVGSDIVGHVEKLGADVKTLMEGDRVIVAPGFPSDPTEWGLTPENEAS
                     SYFPTGTHGWGGYAQYVEVPARWVIRNDIDLAAEELATMPLVLVTAVHAVKTLGGVGP
                     GSRVLVQAGASGSGSMAIQVAKALGASVITTVSTPEKAALARSVGADEVVRYRETDVS
                     ASVRAWSGRDGIDVVIDPVGGTTMGANLDCLKPRGTLVNFGFGGGAEATIPHLYPFFR
                     YERRIVGSWMGSMAELLFGLDLV"
     gene            1007..1912
                     /locus_tag="VNN25_27650"
     CDS             1007..1912
                     /locus_tag="VNN25_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXH95382.1"
                     /translation="MFDIKRIDLNLLVVFDALLDERNVTHAAARLGYTQPTVSGMLTR
                     LRDLFGDPLFVRTQRGLLATPRAEELTAPLKQLLADSRRLVARDVFDPASAEMTFTIS
                     SNDYMQRTLLVPFIKVLRSEAKQVRLAIAPPIIEGIGDALARGQIDMAVTIAEFAMPD
                     LLSRLLYRERYVVAVRRQHPLARGSTMTVERFCNYDHVLVSPTGGSFEGPADRALAKL
                     KQRRKVRYSVPSFLLVPEILQTDDLVALVPSRLVREHKKQLVVFNPPIEVPGFDVIAV
                     WHPRVHKAMAHRWLRSRLAEIGKIP"
     gene            2324..2665
                     /locus_tag="VNN25_27655"
     CDS             2324..2665
                     /locus_tag="VNN25_27655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95383.1"
                     /translation="MAELRAQGKSPEEIRRFVADAFAQGIFQAPTRTGVDYMLSKENL
                     TPNARAGVVPFLPHLMFYAPYLTNADLGLDGTLGPDGNPVGPAFVAGGPHALIIVPIG
                     PHQSAGHSTAQ"
BASE COUNT          544 a          848 c          750 g          526 t
ORIGIN      
        1 gacgagatct agcccgaaca gcagttcagc catgctaccc atccaggacc caacgatccg
       61 ccgttcgtaa cggaagaacg gatagagatg tggaatcgtc gcctcagcgc cgccgccgaa
      121 cccgaaattg acgagggtgc cgcgcggctt cagacagtcc agattggccc ccatggtggt
      181 gccgccgact ggatcaatga cgacgtcgat gccatcgcgg ccggaccagg cacgcaccga
      241 cgcgctaacg tcggtctccc gatagcgaac gacctcgtca gcccccacag atcgcgcgag
      301 cgcggccttc tcaggagtgc tgaccgtcgt gatgacgcta gcgccgagag ccttggctac
      361 ctgaatcgcc atactgccgc tgcctgaggc gcccgcctga accagtaccc ggcttcctgg
      421 gccaactcct ccaagggtct tgactgcatg aaccgcggtg acgagcacga gcggcatcgt
      481 ggcgagttcc tccgccgcaa ggtcgatgtc gttgcggatc acccaacgtg ccggcacttc
      541 gacgtactgc gcatagccgc cccacccgtg cgttcccgtt gggaagtagc tcgacgcttc
      601 gttctccggc gtgaggcccc attccgtcgg atcggacgga aaacccggtg ccacaatgac
      661 ccgatctccc tccatcagtg tcttgacgtc ggcaccaagc ttctcgacgt gaccaacgat
      721 gtcggagccg accacatgtg gaagcggaat caaccggctg acggcgccgg agcgcaccag
      781 cgtgtcgtaa tagttggtac caactgcctc gaccctgatg agcacgtgac caggcctcgg
      841 agcaggggtc gggacttcac cgaattcaag aacgtcgata tcgccgaagg cagtcattgt
      901 gacggcttgc atagcgtaat ctccttgggg aagttttgag ttccaggcta aagtagccgc
      961 cggcccggca ttgctccaat cgcaaatctt tggatctggt atagccatgt tcgatatcaa
     1021 gcggatcgat ctcaacttgc tcgtagtctt cgatgcgtta ctcgatgagc gcaacgtcac
     1081 gcacgcagcc gctcggctgg gctacaccca gccaacggtc agtgggatgt tgacgcgcct
     1141 tcgtgacctc ttcggcgatc ctctgtttgt acgcacgcag cgcggactgc tggcgactcc
     1201 acgagcagag gaactgaccg cgccattgaa gcagttgctc gccgacagcc ggcgactcgt
     1261 cgcacgcgac gtattcgacc ctgccagtgc cgagatgacc tttacaattt ccagcaacga
     1321 ctacatgcag cgcacgcttc tcgtgccttt cattaaagtg ttgagaagtg aggcgaaaca
     1381 ggtccgccta gctatcgcac caccgatcat cgagggcatc ggcgacgcac tcgcgcgggg
     1441 acagatcgac atggctgtca ccattgccga atttgcgatg cctgatctgc tctcacgctt
     1501 gctgtaccgc gagcgctacg tcgtagccgt tcgccgtcag catccgctcg cgcgaggctc
     1561 gaccatgacc gtcgagcgtt tctgcaacta cgaccacgtt ctggtctcac cgacaggcgg
     1621 atcattcgaa ggcccagccg atcgagcgct tgcgaagctc aagcaacggc gcaaagtccg
     1681 ctactccgtc ccctcgttcc tgctcgtgcc ggaaatactc caaacggacg atctggtcgc
     1741 actggttcca tctcgtctgg tgcgcgagca caagaagcaa ctggttgtgt tcaatcctcc
     1801 cattgaggtt ccgggttttg acgtgatcgc ggtatggcat ccgcgcgtcc acaaagcaat
     1861 ggcccatcgc tggctacgta gtcgcctcgc ggagatcggc aagatcccgt agcgtggatc
     1921 gcgcatgacg cagcgcctcg cagcgctgat tcacagttag atacctttgc tcgcttcgtg
     1981 gtgggccaag gacgagacag gctcggacat cgcatgtcat cgagtccgag ctagtgcgaa
     2041 tcaaagaatc aaagccaaat cacttttcac tcatatcaac tcatggctcc gaaacgctgt
     2101 gccacatatg tgaccaactc agatcgcact ggctctcagt gcttgcccgg agtcgatagc
     2161 aagtaaagcg gcggtctatg tccttgagag gtctggctac gtcaaggttc gggagagcca
     2221 aaacgggttt accgctattg tccagcacgc gctgcccaac agccaagatc cgcagtgcat
     2281 ggatgccgag ggtacacgca ccttcctccc gcgttatctg aaggtggccg aactacgcgc
     2341 tcagggcaag agtcccgaag agattcggcg cttcgtagcc gacgccttcg cccaggggat
     2401 cttccaggcg cctacacgga cgggtgtcga ctacatgcta tcgaaagaga acctgactcc
     2461 gaacgcaagg gccggcgtcg tgccctttct gcctcatctc atgttttatg ccccgtacct
     2521 aaccaatgcc gacctcggct tagatggcac tctcggcccc gacgggaatc ctgtggggcc
     2581 ggccttcgtc gcgggtggtc cgcacgcgct aattatcgtt cccattgggc cgcatcagag
     2641 tgcgggtcac tccacggcgc aataagcc
//
LOCUS       DATLVW010000934         4972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_575738, whole genome shotgun sequence.
ACCESSION   DATLVW010000934 DATLVW010000000
VERSION     DATLVW010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575738"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..544
                     /locus_tag="VNN25_27660"
     CDS             191..544
                     /locus_tag="VNN25_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95384.1"
                     /translation="MPFMNRKYETDRSQSGNRGFRSPMTDFNRQSRGNGNGSARPSLK
                     ATPPETTNAESFYYLKQMSSRTPMVIVMTDGEEIHGSIEWYDKTSLKVNRTGAPNLLI
                     QKQWIKYLFKQEELD"
     gene            674..1588
                     /gene="pdxA"
                     /locus_tag="VNN25_27665"
     CDS             674..1588
                     /gene="pdxA"
                     /locus_tag="VNN25_27665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXH95385.1"
                     /translation="MKPLAITLGDPAGIGAEIVFKALRETDVPAIVFGSRALADPPDG
                     IEFVDVGGEAPVRYGVIDATYGRIALDSIDAALKGIRSGQCSALVTAPIHKQSIALAG
                     SPFPGHTELLAARAGLRRYAHHYAMYFDSPRLKSVLLSVHLPLREAISQINFVDIVGL
                     ADLMNREYKRIYGQTPRIAVAGVNPHAGEGGKFGDEERVIGKAVAHARKLGLTITGPH
                     PADTVFLSAHRGQYDVVLAMYHDQALIPIKAIAFDESVNVTIGLPYLRVSVDHGTAFD
                     IAGRGIADAAPMRYAISWAATHAEGYTR"
     gene            1585..1731
                     /locus_tag="VNN25_27670"
     CDS             1585..1731
                     /locus_tag="VNN25_27670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95386.1"
                     /translation="MNRWTARILGLILLIVFLTLMLNLEKQLQMLQKSQGGSGAGSTA
                     TTST"
     gene            1772..2227
                     /locus_tag="VNN25_27675"
     CDS             1772..2227
                     /locus_tag="VNN25_27675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95387.1"
                     /translation="MDTNRLLKWIIVIVLAIVAWKYGLPWVKEHMRGSAATSSSPESS
                     CTTTAQHASEIWGSGLHRFANPPYDMAAWSEFRGGVESEIAKAQTECRCSQQSCETTR
                     GAMSDLRSLVSEFDTAIRNGSPPPDNAVQRQESIDTTIETAAALARGGK"
     gene            complement(2228..3517)
                     /locus_tag="VNN25_27680"
     CDS             complement(2228..3517)
                     /locus_tag="VNN25_27680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXH95388.1"
                     /translation="MRRFVVPLLRLIANIFFRRIESVGWDNVPGAPVIFAVNHPNGLI
                     DPLFLLCFTPRPISFLAKAPILRMPLIGWLARGLDTIPVYRTQDNFSTTKNKEMFAAA
                     RALLMRGGAIAIFPEGTTHSEPRLKELKTGAARIALGCGADECPDIFIVPAAIFYTAK
                     QSFRSNALVYYGDPIRVAPVAVDDAGEPPREAVDALTSRIEKAMAELTLQADTREALD
                     LIGRAERVMSLGRAALPEQLDLRKRFIAGYARLRERDPARLEHLQSRFERFEAELGSA
                     NLEPETLPAPTVRGSVRTLSTLLITLPLALLGAIIHYPIYRLIGFIVRRFTHEEELVA
                     TVKAVAGMVFFPLVYAALALAAGLRFGFLYGAVVLVVLPFLGYVALRFFEQLDDVIGR
                     TRSLTWRIARRSAFERLLRTRRELRDEVVALAEEMGV"
     gene            complement(3514..4818)
                     /locus_tag="VNN25_27685"
     CDS             complement(3514..4818)
                     /locus_tag="VNN25_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXH95389.1"
                     /translation="MPAATDTPAAQRAARYALAILALINLFNYLDRWVVAAVVESLKK
                     SLQLSDTQIGLLPTGFIVIYTLTSPLFGTFGDRNARPPLIALGVAVWSVATALGGFAR
                     GFTSLFIARSAVGVGEAAYGTIAPALLADSFPIEKRGRVLAVFFAAIPIGSAAGYILG
                     GLADQHFGWRAAFWIAGAPGLLLALLVMLVKDPPRGLHDVGGEWGVGGRESETDANCT
                     MSGAESSDPRPPTPWTYAYRDLLRNRPFILTALGYGAYTFALGGLGYWMPAFLERVRG
                     MPRSEATVTFGAIALVTGFVGTFAGGWLGDLFLSRSKQSYLWVSGIATLLAAPATYIA
                     VSNPHRGVYLPAIAIAEVFIFMSTGPVNSAIINVVKPGERATALGLSVLVMHLVGDIP
                     SPPLIGLVSDHSTLERAFMLVPLAILVAGGIWCYTAWWGRQR"
BASE COUNT         1028 a         1582 c         1543 g          819 t
ORIGIN      
        1 tgatacgcgt ctcggtgtcg ggcacctgcg cgatacacac tacggaattc aaggtaggcc
       61 tccgggtaaa gcgggccgga ttctagggaa aaggcacgag gttcgcaaac aaggctccag
      121 tttccaaacg gagagagtcg gtcaccagcg agcaacggcc tcgccaaagg cagccgaacg
      181 gaggattgcg atgccattca tgaaccggaa gtacgagacg gatcggagcc agtcgggtaa
      241 tcggggattc cggtcaccga tgaccgactt caaccggcag tcgcggggaa acgggaacgg
      301 cagcgcacgc ccgagtctga aagcgacgcc tccggaaacg accaacgcgg agagtttcta
      361 ctacctcaag cagatgagct cgcgcacgcc gatggtgatc gtcatgaccg acggcgaaga
      421 gatccacggc tcgatcgagt ggtacgacaa gacctcactg aaggtgaatc gcaccggcgc
      481 tccgaatctc ctcattcaga aacagtggat caagtatctc ttcaagcagg aagagctcga
      541 ttagcgcgat caccggggga acggattcac gcggagatgc ggagcagaga gaaccactcc
      601 gcgactccgc gtctccgcgt gacccgttct ctcgcccgtt cagccgaact tcccccgcac
      661 tacaatccag ttcgtgaaac cgctcgcgat cacgctcggc gatccggcag gtatcggcgc
      721 tgagatcgtt ttcaaagcgc tccgggagac cgatgttcca gcgatcgtct ttggtagccg
      781 ggctctcgcc gatccgcccg acggcatcga gtttgtcgat gtcgggggag aggctccggt
      841 gcgctacggc gtcatcgatg ccacctacgg tcgcatcgcc ctcgactcga tcgacgccgc
      901 gctcaaaggg attcgcagcg gccaatgctc ggcgcttgtg accgcgccga ttcacaaaca
      961 gtcgattgct cttgccggct cgccgttccc cggccacacg gagctgctcg ctgcgcgcgc
     1021 cggcttgaga cgttatgcac accattacgc gatgtatttc gattcaccgc gtctgaagtc
     1081 cgtgctgctg tcggtgcatc tgccgttgcg cgaggcaatc tcgcaaatta acttcgtcga
     1141 catcgttggc ctggccgacc tgatgaatcg agagtacaag cgcatttacg gccaaacgcc
     1201 ccggatcgcc gtcgcgggcg tgaatccgca cgcgggcgaa gggggaaagt tcggcgacga
     1261 agagcgcgtc atcgggaagg cggtagcgca cgcgcggaaa ttggggctga ccatcaccgg
     1321 ccctcatccg gccgataccg tcttcctgtc tgcgcaccga ggacagtacg atgtggtgct
     1381 ggctatgtat cacgatcaag cgctcatccc gatcaaggcg attgcgttcg atgaatcggt
     1441 gaacgtcacg atcggcttgc catatcttcg cgtttccgtc gaccacggaa cagcgttcga
     1501 catcgccgga cgtggcatcg ccgatgcggc gccaatgcgc tacgcgatca gctgggcggc
     1561 tacgcatgcc gagggctaca cgcgatgaac cgctggaccg cccgcatact cgggctcatt
     1621 ctcctcatcg ttttcctgac actgatgctc aatctcgaga agcagcttca gatgctgcag
     1681 aagagtcagg gcggaagcgg cgccggctcg acggccacaa cgtcgacgtg aacggtccgc
     1741 gctcatcacc gcgcaggcgt aagatcagtg gatggatacg aaccgcctgc tgaaatggat
     1801 catcgtcatc gtgctggcga tcgtcgcctg gaagtacgga ctgccgtggg tgaaggaaca
     1861 catgcgcgga tccgccgcca catcttcttc ccccgagagc tcatgcacca ccaccgccca
     1921 acacgcttcg gagatctggg gcagcgggct ccatcgcttc gccaatccgc catatgacat
     1981 ggccgcgtgg tcggagtttc gcggcggcgt ggagtctgag atcgcgaaag cccagacgga
     2041 gtgccgttgc tcgcagcagt cgtgcgaaac aacgcgcggc gcaatgagcg atcttcgcag
     2101 tctcgtttcg gaattcgaca ccgcaatccg gaacggctcg ccgccgccgg ataacgcggt
     2161 gcagaggcag gaatcgatcg atacgaccat cgagaccgcc gcggcacttg ctcgcggcgg
     2221 caagtagtca gacccccatc tcctccgcca acgccacgac ctcatcacgc agctcgcgcc
     2281 tcgtgcgcag cagtcgctcg aacgcacttc gccgcgcgat gcgccaggtg agcgagcgcg
     2341 tgcgaccgat cacgtcgtcg agctgttcaa agaaacgaag cgcgacgtag ccgagaaacg
     2401 ggagaacgac cagcacaacg gcgccgtaca ggaatccgaa acgaagaccg gccgcgagcg
     2461 cgagcgcggc atacacgagc ggaaagaaga ccatgcctgc gaccgccttc accgtcgcaa
     2521 ccagctcttc ctcgtgcgtg aatcgcctga ctatgaatcc gatcagccga tagatcggat
     2581 agtgaatgat ggctcccagc aacgcgagcg gcagcgtgat cagcaatgtc gacagagttc
     2641 gcacactacc tcgcaccgtc ggcgccggca gcgtctccgg ctcgagattc gcgctgccaa
     2701 gctccgcctc gaatcgctcg aaccgcgatt gcagatgttc cagccgcgcg ggatcacgct
     2761 cgcgaaggcg cgcgtagcca gcgatgaagc gtttgcgcag atcgagctgc tccggaagcg
     2821 cagcacggcc aagcgacatc acacgctcgg cgcgtccgat gagatcgagc gcttcgcgcg
     2881 tgtctgcctg cagcgtcaac tccgccatcg ccttctcgat gcgcgatgtg agtgcatcga
     2941 ccgcctcgcg cggcggctcg ccggcatcat cgaccgccac cggcgcgact cggatcggat
     3001 cgccgtagta aacgagcgca ttgctgcgga aactttgctt tgccgtgtag aagatcgccg
     3061 ccggcacgat gaagatatcg gggcactcgt ctgctccgca gccgagggcg atgcgcgcgg
     3121 cgccggtttt cagctccttc agccgcggct cgctgtgcgt cgtcccctcg gggaaaatcg
     3181 cgatcgcgcc gccgcgcatc agcagcgcac gcgccgccgc gaacatctct ttgttcttcg
     3241 tcgttgaaaa attgtcctgc gttcggtaga ccggaatcgt gtccagtcct cgggcgagcc
     3301 aaccgatgag cggcatgcga aggatcggtg ccttggccag gaacgagatc ggccgcggcg
     3361 tgaaacaaag gagaaagagc ggatcgatca gaccgttggg gtgattgaca gcgaagatca
     3421 ccggcgcgcc gggaacgttg tcccatccga cactctcgat gcgccggaag aagatgtttg
     3481 cgatcagccg cagcaacgga acgacgaagc gtctcaacgc tggcggcccc accatgccgt
     3541 gtagcaccag atgccgccag cgacgaggat ggcgagcggc acgagcatga acgcgcgctc
     3601 cagcgtcgag tgatcggaga cgagaccgat cagcggcggc gaggggatgt cgccgacgag
     3661 atgcatcacg agaacggata agccgagcgc tgtggcccgc tcgccgggct tgaccacatt
     3721 gatgatcgcg gagttcaccg gtcccgtcga catgaagatg aacacctcgg cgatagcgat
     3781 cgccggcaga tacacgcccc gatgcggatt cgaaaccgcg atgtacgtcg cgggcgccgc
     3841 cagcaacgtg gcgatacctg agacccacag gtaggactgt tttgatcgcg ataagaagag
     3901 atcgcccagc cacccgccgg cgaacgttcc gacaaaccct gttaccagcg cgatcgcgcc
     3961 aaatgtcacc gtcgcttcac tccgcggcat gccgcgcacg cgctccagaa acgcgggcat
     4021 ccagtacccg aggccgccga gtgcgaaggt gtacgcgccg tagccgaggg ccgtgaggat
     4081 gaaggggcga ttgcggagaa gatcgcgata tgcgtaggtc cagggagtcg ggggtcgggg
     4141 gtcggagctt tctgctccgc tcatcgtgca gttcgcgtct gtctccgact cccgaccccc
     4201 gactccccac tccccgccaa catcgtgcaa tcccctcggc gggtccttca ccagcatcac
     4261 gagaagcgcg aggagtaggc ccggcgcgcc ggcgatccag aacgcggcgc gccagccaaa
     4321 gtgctggtcg gcgagaccgc cgaggatgta gccggctgcg gaaccgatcg ggatggcggc
     4381 gaagaatacc gccaggacac gtccgcgctt ctcgatcgga aacgagtctg ccaggagtgc
     4441 cggcgcgatc gttccgtagg cagcctcgcc gacgcccacc gccgaccgcg caatgaaaag
     4501 cgaagtgaag ccccgcgcga atccccccag cgcggtcgcc acactccaaa ccgccacgcc
     4561 gagtgcgatt agtgggggcc gggcgttccg gtcgccaaat gtcccgaaca gaggcgatgt
     4621 caacgtgtag atgacgatga acccggtcgg gagaagaccg atctgcgtat ccgagagttg
     4681 cagcgacttc ttcagcgact cgacaaccgc ggcgaccacc catcgatcga ggtagttgaa
     4741 aaggttgatg agcgcgagga ttgccagcgc gtatcgcgcg gcgcgctgcg cggcaggcgt
     4801 atcggtcgct gctggcatcc acagaagtgt aacggcgaaa ctcgccgagc gccggaacgt
     4861 attcaaagag cgcggttatc atggcgctta ctcactcatc atgaaacgag cccttcccta
     4921 cctggcgtgc attgccggca tcgtcgcgct cgtgctgatc attccgcgct tc
//
LOCUS       DATLVW010000935         3699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_575840, whole genome shotgun sequence.
ACCESSION   DATLVW010000935 DATLVW010000000
VERSION     DATLVW010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575840"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..907
                     /gene="trpA"
                     /locus_tag="VNN25_27690"
     CDS             98..907
                     /gene="trpA"
                     /locus_tag="VNN25_27690"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXH95390.1"
                     /translation="MNRVRKLFTRLRAGHRCGLIAYVTCGDGPTVEIVHALEEAGADA
                     IELGVPFSDPIADGPVIQMAAQRALKNGTNIGDVFAIADEVRTRSDIPLIAFSYLNPV
                     LRYGVDRFADDAATAGIDAVLLTDLPAEAAAEVRPSFRKRGLGSIFLLAPTSSDARIA
                     AVNRVSDDFIYYVSTTGVTGTRNELDPTLLTRLDEVRARVNKPLAVGFGISRHEHFEL
                     LKDRCDAIVVGSAIVRAVGNGDPAGAPARAAAVVKTILDRDAPAPLLSPAT"
     gene            1045..1338
                     /locus_tag="VNN25_27695"
     CDS             1045..1338
                     /locus_tag="VNN25_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HXH95391.1"
                     /translation="MDRTDAEQELTRLRDAIDRVDEVIVRLLNQRAKYAIEIGEIKAV
                     LDMPIYAPEREKQVLLHVERTSDGPLDPATVRRLFERIIDESRRVERHATDRS"
     gene            1359..2372
                     /gene="aroF"
                     /locus_tag="VNN25_27700"
     CDS             1359..2372
                     /gene="aroF"
                     /locus_tag="VNN25_27700"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXH95392.1"
                     /translation="MVIVMQKHASDTDIEHVVAVLSERGFDVHRSTGSDQTVLGVVGD
                     VASVDPREFEVLHGVQEAVRVSEPYKLSSRSFRREKTVVKVRDISIGGSDVVVMAGPC
                     TIENERQLFETARRVARAGARVLRGGAYKARTSPYAFQGMGVEGLKLLRAAGDENNMA
                     TISEVMEISQIEAMLEYVDILQVGARNMQNYNLLSALGQIRKPVLLKRGMSATIQEWL
                     LASEYILSGGNYDVILCERGIRTFETYTRNTLDISAIPVINKLSHLPIIADPSHAVGR
                     RDKVMPLARAIVAAGADGVLIEVHHEPEKAVCDGPQSLYPDQFARLMDELRIIVPAAG
                     RTL"
     gene            2526..3542
                     /locus_tag="VNN25_27705"
     CDS             2526..3542
                     /locus_tag="VNN25_27705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95393.1"
                     /translation="MNRQPVMRLSTLRSFACLVVIGLAGLSIPDAQAQQVLWVGNDNV
                     AGGSQQYALPISGSSTPNFTVVSSNVVAMAQDGSGNLVMGDFLGQLKFFTAPLSGAST
                     ASATFANGAGPNTGQFAFTPTGDFFVGTVGTAVNKFTHPFSNASTPSQVITTAGMTSA
                     IGVALDAAQNMYISDAGGSGSSIFVLAPPYTGAPIHTPAISGKFYRKVAVSGTQLFVA
                     DVSGTTGRVDVYNLPITAVSAPAFSITNGTNTPETVALDVSGNLYVGNLGNSTVTVYS
                     SPFSAASAPVTTMAAAGAPAIFGIAIGSTVGSPSIPAIGHLGLMALCALMMVIGLVAV
                     RTKS"
BASE COUNT          689 a         1223 c         1120 g          667 t
ORIGIN      
        1 cccccacaaa aagcgtgggg gagaaggacg ctcgattggg agtcttgtgt gattccatca
       61 acggcgcccg cggcacgagc cggctcaggg tggccgcatg aaccgcgtcc gcaaactctt
      121 cacccgcctt cgcgccggcc atcgttgtgg gctcatcgcg tacgtcacct gcggcgacgg
      181 tccgaccgtc gagatcgttc atgcgctgga agaggcgggc gcggatgcga tcgagctcgg
      241 tgtgcccttc tccgatccga tcgccgacgg cccggtcatc cagatggccg cccagcgtgc
      301 gttgaagaac gggacgaata tcggcgacgt cttcgccatc gcggacgagg ttcggacgcg
      361 gagcgatatt ccgttgattg ccttctccta tctcaatccc gtgctgcgat acggcgttga
      421 cagattcgca gacgacgcgg caacggccgg catcgatgcg gtgcttttga cggacctgcc
      481 agcggaagcg gcggcggagg tcagaccgtc attcagaaag cgcgggctgg gatcgatttt
      541 cctgctggcg ccgacatcga gtgatgcacg gattgcggcc gtaaatcgcg tcagcgacga
      601 cttcatctac tacgtctcca cgacaggcgt caccggaacg cgcaacgagc tcgatccgac
      661 gttgttgacg cggctcgatg aagtgcgcgc tcgcgtcaac aagccgcttg ccgtcggatt
      721 cggtatatcg aggcacgagc acttcgagct cttgaaagac agatgcgatg ccatcgtcgt
      781 cggcagcgcg atcgtgcgcg cagtggggaa cggcgaccct gcgggagcgc cagcgcgcgc
      841 ggccgccgtc gtcaaaacga ttcttgacag agatgcaccc gcgcccttac tctcacccgc
      901 cacatgactc tcaccgccca gcgctttgcg tattactacg gctactttac gtaagcgtgg
      961 ggcaaatatc gcgccccgct acgaaggggt gctgaccgct gagtcgatcc gaacctcttc
     1021 ccgcgcggca agaggttttt tcttatggat cgaacggacg cagaacaaga gctcacccgt
     1081 cttcgcgacg ccatcgaccg cgtcgacgag gtcatcgtgc gcctcctcaa tcaacgcgcg
     1141 aaatacgcga tcgagatcgg cgagatcaag gcagttctcg atatgcctat ctacgccccc
     1201 gagcgcgaga agcaggtcct cctgcacgtc gagcgcacct cggacggccc gctcgatccc
     1261 gcaactgtga ggcgtctttt tgaacgaatc atcgatgaat cgcggcgcgt agagcgccac
     1321 gcgacggatc gcagttagag agaggcaggg aggatttcat ggtcattgtg atgcaaaaac
     1381 acgcgtcgga tactgacatc gagcacgttg ttgcggtgct cagcgaacgc ggtttcgacg
     1441 tacaccgctc caccggttcg gatcagacag ttctcggcgt cgtcggcgat gtcgccagcg
     1501 tcgacccgcg cgagttcgag gtgctgcacg gcgtgcagga agcggtgcgc gtcagcgagc
     1561 cgtacaaact ctccagccgc tcgttccggc gcgagaagac cgtcgtaaaa gtgcgcgaca
     1621 tctcgatcgg cggcagcgac gtcgtcgtca tggccggccc gtgcacgatc gagaacgagc
     1681 gacaactgtt cgaaaccgca cgacgagttg cccgcgccgg agcgcgcgtc ctgcgcggcg
     1741 gcgcgtacaa ggcgcgcacc tcgccgtacg cgttccaggg catgggcgtc gaaggactga
     1801 aactgctgcg cgccgccggc gatgagaaca acatggccac gatctccgag gtgatggaga
     1861 ttagccagat cgaagcgatg ctggagtatg tggacatcct gcaggtcggc gcacgcaaca
     1921 tgcagaacta caacctgctc tccgccctcg gccagatccg caaaccggtg ctgctcaaac
     1981 gcggcatgtc cgccacgatt caggagtggc tcctggcctc ggagtacatc ctctccggcg
     2041 gcaactacga cgtcatcctc tgcgagcgcg gcatccgcac gttcgagacc tacacacgca
     2101 acaccctcga catctccgcg atcccggtca tcaacaaact gtcgcatctc ccgatcatcg
     2161 ccgacccgtc gcacgccgtc ggccgccgcg acaaagtgat gcccctggcc cgcgccatcg
     2221 tcgccgccgg cgccgacggc gtcctgatcg aagtccacca cgagcccgaa aaagccgtct
     2281 gcgacggccc ccagtcgctc taccccgacc agttcgcaag actaatggac gaactgcgga
     2341 tcatcgtccc cgcagcggga cgaacattgt gacgggcagt agcgtagcgc ccgcgaacgt
     2401 agcgccggcg gcccgccggc tgttccgccg ccggcccggc ggcacgtgcg gagttacgta
     2461 cccaacatta gcttcctctc cggattctca tccgttccgc gtttcgctcg cgtacactag
     2521 ttcgcatgaa tcgtcagccg gtaatgcgtc tgtccacact caggtccttc gcttgtcttg
     2581 tcgtgattgg cctcgccggt ctctcgatcc ccgacgcgca ggcgcagcag gtgctgtggg
     2641 tcggcaacga caatgtggcg ggcggatcgc agcagtacgc gctgccgatc agcggcagct
     2701 cgacgccgaa cttcacggtc gtatcgagca atgtcgtcgc aatggctcag gacggcagcg
     2761 ggaatctggt catgggcgac ttcctgggac aactgaaatt cttcaccgcg ccgctgtcgg
     2821 gggccagcac agcctccgcg accttcgcga acggtgcggg gccgaatacc ggccagttcg
     2881 cgttcacccc cactggcgac ttctttgtcg ggacggtcgg taccgcggtg aacaaattca
     2941 cccatccgtt cagcaacgcc agcacaccat cgcaggtgat caccaccgcc gggatgacct
     3001 cggcgatcgg cgtggcgctc gatgccgcac agaacatgta catctccgac gccggtggtt
     3061 ccggcagcag catcttcgtc ctggcgccgc cgtacacggg agcgccgatc catacgccgg
     3121 cgatctccgg caagttctac cgcaaggtcg ccgtcagcgg gacgcaactc ttcgtcgctg
     3181 acgtctcggg aacgacgggg agggtggacg tctacaacct cccgatcacg gcggtgagcg
     3241 cgcccgcctt ctcgatcacg aacggcacga acacgccgga aaccgtggcg cttgatgtat
     3301 caggaaacct ctatgtgggg aacctcggca actcgaccgt taccgtctac agttcgccat
     3361 tctcggccgc cagcgctccc gtgacgacga tggccgcagc cggcgcgccc gcgatcttcg
     3421 ggatcgccat cggcagcact gtcggttcgc cgtccattcc ggcgatcggc cacctgggtc
     3481 tcatggcact ctgcgcgctg atgatggtca tcggcctcgt ggcggtacgc acaaagtcgt
     3541 aattcgacct gaccctttgc tctgcctgcg ggacactcga tgaccgcggt tcactttcgc
     3601 agccctgtct gcgcctcact tactgatctg cagcaggtgt agtgatgctc gtgattgggc
     3661 ggccggcatc ggcttgtcgc ccttgatcat caaccagag
//
LOCUS       DATLVW010000936         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_493016, whole genome shotgun sequence.
ACCESSION   DATLVW010000936 DATLVW010000000
VERSION     DATLVW010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493016"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VNN25_27710"
     CDS             complement(<1..1342)
                     /locus_tag="VNN25_27710"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXH95394.1"
                     /translation="MATLFTAAAILLLGTAVLIRTRVTLVVRLFFVLSVAASGWLCCY
                     GLMYNAASAESALIFARIGGAFSSLLPAATFHFAASYTSRRGALRNYILFCWMFCGGI
                     ALLQLATPYFISGMRQFDWGFFPIGPRYNLSWILVFSAMFVVAIWMLWKVMDSSDGLE
                     RDRARALVIAFSVAMLSGVDFLPTIGINVPPFGFMPVLAFVAIAAHAVWRYRLVDLTP
                     EYAASQILATMKGAVIVVDLEGKIRVANHAASVMLGYRDPGLIGKHLRNVVSPDENLT
                     TGQLLHSLGMLEQNMVWSTAAGGRIDVLATSSFVRDEGGDPVGVVYAATDITERRRAE
                     QRLRESEERYRLLFERNLAGVYRSSLDGSILDCNDALARTFGFSNRGELLGQSAFSLY
                     NAAEDRQRLLAQVREHGSLSNAEVRMRRRDGSPVWVSENMTLLEGGILEGTILDI"
     gene            1523..2836
                     /locus_tag="VNN25_27715"
     CDS             1523..2836
                     /locus_tag="VNN25_27715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH95395.1"
                     /translation="MQISTMTAPHFARRVSLLGSENAFKIAPLIREIEQRGERVIKCH
                     VGEPDFPLPGHIAREVKRQIDLGMTHYGDPQGLESLRATVARDVGASRGIKITPERVV
                     IFPGAKPPIGMAQHIYCDPGDEVIYPSPGFPIYESFTRYLGAVPVPMHLHHENGFTCG
                     AAELAPLISERTRLIYLNFPSNPTGGVATREQLEELADVILRRGPDDVRVYSDEVYER
                     ILFDGARHHSIASLPGMEERTIIVSGVSKTYAWTGGRVGWAILPTVEEAQAFLNLNIN
                     YLSCVSGYNQLGAKLAIESPASEPEIKAMVAEFQHRRDVVVERLNSIPGVTCPTPKGA
                     FYLFPDIAGVCERVGAVEAWNRLPRSAREASSPSTLFQRYLLFDHHVATLDRRSFGAI
                     GHEGKHYLRISIATGLEDLMEATFRIALAADNPEGFQSFVARMTP"
     gene            2833..>3949
                     /locus_tag="VNN25_27720"
     CDS             2833..>3949
                     /locus_tag="VNN25_27720"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95396.1"
                     /translation="MTTASIEPQGATRIDLARFNRTRLDLDALRQDEVDALETLDLAY
                     RSLCAMLYNYAPLSGHPGGSISAGRILSMLFFRTMDYDALDSGRDDADVLSLGAGHKA
                     LGLYAMSALRSEILRIAGVRREGTGDLRLEDLLGFRRNPTTTTPLFRVLGAKALDGHP
                     TPATPFVPLATGASGVGMASSLGYALAAFDWFGEAAPRVHILEGEGGLTPGRVAEALA
                     FAGTASLGNVVVHVDWNQSSIDSDRVTREGTERGDYVQWDPAGLFHLHDWNVVSVPDG
                     TNLQQIAAAQRMALLSDNNRPTAIVYRTQKGWRYGIEGRASHGAGHKLCSHGFFDALS
                     IPAGDIPCCELNSSLCDDGTNAVMVEQCYWQALSTIRN"
BASE COUNT          755 a         1281 c         1220 g          693 t
ORIGIN      
        1 tgatgtcgag gatggttccc tcgagaatcc cgccttcgag cagcgtcatg ttctcgctga
       61 cccacaccgg cgagccgtcg cggcggcgca tgcgcacttc ggcattggag agtgagccgt
      121 gctcgcgcac ctgggcgagc aacctctgac gatcttctgc ggcgttgtag agcgagaacg
      181 cggactgccc gaggagctct ccgcgattac tgaagccgaa ggtgcgcgcc agggcatcgt
      241 tgcagtcgag gatggagcca tcgagtgagc ttcgataaac gccggccaga ttgcgctcga
      301 acagaagccg gtagcgctcc tcgctctcgc ggagacgctg ctcggcgcgg cggcgttcgg
      361 tgatgtcggt cgccgcgtag acgacgccga ccggatcgcc cccctcatcg cgcacaaatg
      421 acgatgttgc gagcacgtcg atgcgcccgc ccgcggccgt gctccagacc atgttctgct
      481 cgagcatgcc gagactgtga aggagttggc cggtggtgag attctcatcc ggcgagacca
      541 cgttgcggag gtgtttcccg atcagacccg gatcgcgata gccgagcatc accgacgctg
      601 cgtgattggc aacgcggatc ttcccctcca gatcgacgac gatcaccgca cccttcatcg
      661 tcgccagaat ctggctcgcc gcatactccg gcgtaaggtc gacgagccga taacgccaga
      721 cggcatgtgc ggcgatggca acgaaagcca gcaccggcat gaaaccgaac ggaggaacgt
      781 tgatcccgat cgtcggcagg aagtcgacgc cggacagcat ggccacgctg aaggcgatga
      841 cgagcgcacg ggcacgatcc cgctcgagtc cgtcgctgga atccatcacc ttccagagca
      901 tccagatcgc cactacgaac atggcggaga agaccaggat ccatgagagg ttgtagcgcg
      961 gtccgatcgg gaagaatccc cagtcgaact gccgcatacc gctgatgaag tacggcgttg
     1021 ccagttgcag cagcgcgatc ccgccgcaga acatccagca gaagagaatg tagttgcgca
     1081 gcgcgccgcg gcggctcgtg tacgaggctg cgaagtggaa ggtggctgcc gggaggaggc
     1141 tggaaaaagc accgccgatg cgcgcgaaga tcagcgcact ctcggcactc gcggcgttgt
     1201 acatgaggcc gtagcagcaa agccacccgc tcgcggcgac cgacagcacg aagaagagtc
     1261 gcacaacgag ggtgacacgg gtccggatca gaacggcggt gccgaggagg agaatcgccg
     1321 ccgccgtgaa aagcgtggcc accgcgagcg cgctggcggc acggtccgga aactgtatga
     1381 gagcgacgac aaaatgctcg atgcttcagt gtaacccgac tcgaatgcgc aggccgagtt
     1441 gcgccacccg atgcgaggtt catcacacct ccttgtgagc cgtggcactg tgacgggacg
     1501 ttcgtcgcag gacactgcct ctatgcaaat cagtacgatg acggcaccgc actttgcgcg
     1561 acgtgtgtct cttctcggct ccgagaatgc gttcaagatc gcgccgttga tccgcgagat
     1621 cgagcagcgt ggcgagcgcg tcatcaaatg ccacgtcgga gagcccgatt ttccgctccc
     1681 cggccacatc gcccgtgagg tgaagcgcca gatcgacctc ggcatgacgc attacggtga
     1741 cccgcaaggg ctggagtctc tgcgagccac ggtggcccgc gatgtgggcg cttcgcgcgg
     1801 aatcaagatc acgccggaac gcgtcgtgat ctttcccggc gccaaaccgc ccatcggaat
     1861 ggcgcagcac atctattgcg atcccggcga cgaggtcatc taccccagtc caggttttcc
     1921 aatctatgaa tcgttcacac gctatctggg cgccgtaccg gtaccgatgc acctgcatca
     1981 tgaaaacgga ttcacgtgcg gtgccgccga gcttgcgccg ctgatcagcg agcgcacgag
     2041 gctcatctac ctcaacttcc catcgaaccc taccggtggc gtggccacgc gcgaacagct
     2101 cgaggagctt gcggatgtga ttctccggcg cggtcccgac gatgtccgcg tctattccga
     2161 cgaggtgtac gaacggatcc tcttcgacgg agcgcggcac cattccatcg cctccctccc
     2221 gggaatggaa gaacggacga tcatcgtgtc gggcgtgtcg aagacctacg catggacggg
     2281 cggacgcgtc gggtgggcga tcctccccac cgtggaggaa gcgcaagctt tcctgaacct
     2341 caacatcaac tatctctcgt gcgtctccgg ctacaaccag ctcggcgcga aactcgccat
     2401 cgagtcgccg gcgagcgaac cagagatcaa ggcgatggtc gcggagttcc agcaccgccg
     2461 cgacgtcgtg gtggaacggc tcaactcgat ccccggcgtt acgtgtccta cgccaaaagg
     2521 ggccttctat ctctttcctg acatcgccgg cgtctgtgaa cgtgtcggcg cggtcgaggc
     2581 atggaacagg cttccccggt ccgcgcgcga agccagctct ccgtccacgc ttttccagcg
     2641 ctatctgctc ttcgaccatc acgttgccac gctggaccgc cgttcgtttg gtgcgatcgg
     2701 ccacgaagga aagcactatc tgcgcatctc gatcgccacg ggcctcgagg atctaatgga
     2761 agcgacattc cgcatcgctc tcgccgcgga taacccggag gggttccaga gcttcgtcgc
     2821 aaggatgacg ccgtgacgac ggcatcgatc gagccgcagg gcgccacgcg catcgatctc
     2881 gcgcgtttca atcgtacccg tctcgatctc gacgcgctcc gtcaggacga ggtcgatgcg
     2941 ctggagacgc tcgatctggc ctatcgctct ctttgcgcga tgctctacaa ctacgcgccg
     3001 ctgtccggcc acccgggcgg atcgatctcc gcgggccgca ttctctcgat gctgttcttc
     3061 cggacgatgg attacgacgc actcgattcc ggccgcgacg acgccgacgt cctctccctc
     3121 ggcgccggtc acaaggccct tggcctctat gcgatgtcgg cgctgcgctc cgaaatcctt
     3181 cgcatcgccg gagtacggcg cgaaggaacc ggcgaccttc gcctggaaga ccttctcggc
     3241 ttccgccgca atccaaccac caccactccc ctcttccgtg ttctcggcgc gaaagcactc
     3301 gacggccacc ccacaccggc gacacctttc gtgccgctcg ccaccggcgc atcgggcgtc
     3361 ggcatggcct cctcgctcgg atacgcactg gcagccttcg attggttcgg tgaagccgcg
     3421 ccgcgcgtgc acatcctcga gggagaaggc ggccttacgc cgggccgtgt cgccgaggcg
     3481 ctggcattcg caggaacggc ctcgctgggt aatgtcgtcg tgcatgtcga ttggaatcaa
     3541 tcgtcgatcg attccgatcg cgtcacgcgc gagggaacgg agcggggcga ctacgtgcag
     3601 tgggatccgg ccggactctt tcatctccac gactggaacg ttgtgtcggt tccggacggg
     3661 acgaatctgc agcagatcgc ggccgcacag cggatggcgc tgctcagcga caacaaccgg
     3721 ccgacggcca tcgtgtaccg cacacaaaag ggctggcgct acggcatcga aggacgagcc
     3781 tcgcatggtg cgggacacaa gctgtgctcg cacggctttt tcgatgcgct gtccatcccg
     3841 gcaggtgaca ttccctgctg cgagctgaat tcgtcacttt gcgatgacgg cacaaacgcg
     3901 gtgatggtgg agcaatgcta ctggcaggcg ctcagcacca ttcgcaact
//
LOCUS       DATLVW010000937        10488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_536280, whole genome shotgun sequence.
ACCESSION   DATLVW010000937 DATLVW010000000
VERSION     DATLVW010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536280"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VNN25_27725"
     CDS             complement(<1..259)
                     /locus_tag="VNN25_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH95397.1"
                     /translation="MSTNPFPGLRPFQEEEEHLFFGRESQVDAMVDTLAASHFLVVVG
                     TSGSGKSSLVNCGLRPALHRGMMARAGTSWRIAQFRPGNNPI"
     gene            complement(259..1725)
                     /locus_tag="VNN25_27730"
     CDS             complement(259..1725)
                     /locus_tag="VNN25_27730"
                     /inference="COORDINATES: protein motif:HMM:NF013729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXH95398.1"
                     /translation="MSFTRDAFISYAHIDNEPLPDEKEGWVTVFDKALRQVLSRYTGK
                     DANIWRDEKLQGNDVFSDEILSQFPETAVLVSVLSPRYLKSDWCSKEMTQFYEIAEKT
                     GGIIVGNKCRIFKIIKTPFGKDESFPPEVAGVLGRILGYEFYHLDADNTPQELDPAYG
                     DSARQEFLKSVTRVAWHIKELLDVMASGPAAGKARASIYLAECTRDQRDARATLEGEL
                     KRLGYDIVPDSQLPTDSAEYVAEVERLLQQCELSIHLIGSSYGAVPDGTDQSVVALQN
                     QSAVNISRKNGLARVISVPADLQTSDPRQQTFITALHQDAGLQFGADLITGGIEDLKS
                     AVRAALARVERATAAPVADTDKTLVYVICDPRDIQATVPLAKSLIARGMRVELTVFDG
                     DAAAVREANEQLMATAGAIVLFWGAGDDTWRAYQQSDIRKNEGARRDNPPKTITYIVG
                     PATSKKQFLLQTETGVIDALSAPEEQAIAALTEALGLK"
     gene            complement(1911..3233)
                     /locus_tag="VNN25_27735"
     CDS             complement(1911..3233)
                     /locus_tag="VNN25_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXH95399.1"
                     /translation="MTGSLFADDPFEPPARSDAPLPERLRPRSLEEIIGLDEILGEGR
                     FLRTAIESDRIPSMIFWGPPGSGKTTLARIIANKTRAKFVTFSATSTAIKEIRTLMEE
                     ARRERKARGTKTIVFIDEIHRFNKAQQDAFLPYVEAGDIILIGATTENPSFEVNSALL
                     SRTKVVVIPALEREQVMTILRRAVSDPSVRKYGIDVNDDALDFMAATSAGDARQALNS
                     LQLVVETATKADGAIDKTKVTQILQRRSLMYDKGGEEHYNIISALHKSLRNGDADASM
                     YWLVRMLEGGEDPMYIARRLVRAASEDIGNADPQALVFAIAVQQTVHFLGMPEAGVAL
                     GQLVTYLAAAPKSNAVYIGYGEAVREVRQGDNPGVPLHIRNAPTGLMKDLGYGRGYQY
                     AHDFEDQTAAMECLPDALAGRRFYEPKEAGAEAKIKARLDAMRDARKK"
     gene            complement(3258..4220)
                     /locus_tag="VNN25_27740"
     CDS             complement(3258..4220)
                     /locus_tag="VNN25_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HXH95400.1"
                     /translation="MNSSNIDASAVTIEDVREAALRIQPYVHRTPLLSSRSLSEAIGV
                     EVRLKCENLQRAGSFKIRGAMNALLQVPENQRGNGVVAFSSGNHAQGVALAARLLGMK
                     ATIVMPEGSNPSKVAATEAYGATVVTSGVTSATRDTVARDIASKSGASVIPPFDDWRI
                     IAGAGTVGLEIVDEWPDVETIVTPLGGGGLLSGVSIAATSSKPDIAVYGVEPRAGNDG
                     EQSFKSGRIVTIDPPKTIADGARTLAIGERNFAVIRERVHDVVSVEDGVLLDAVKFAM
                     YRTKLVIEPTGALGIAALLSGAIKPKGKTAIVVSGGNLDFSLLC"
     gene            4342..5949
                     /locus_tag="VNN25_27745"
     CDS             4342..5949
                     /locus_tag="VNN25_27745"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXH95401.1"
                     /translation="MWRRVTRGLVLAILVLFVVVPVLTAQTVETHGALEFPDSSVTQS
                     GVILVKGWFLDPASISKVAVYVDDQFVVNSNTNLPRIDVEQSYPNYPGIQHIAPGFQI
                     GMLASRFTNGAHTIAVRVFASDNTITELGRRTISVDNTINQSPFGNVDIPDLAATYNA
                     SGSFPVSGWALDTDGVAKVEVLIDNGVVQNAMYGDPRPDVANTIADWPDALYSAFIAN
                     VDTTRIPDGVHTLDVRATDRLGLSRLIGRRTVQIFNEGGNLKPFGYVDEPKPDAVLYG
                     TRCNVFPQASPAINPQSHITPVRGWALDLGTRADTGRVAYAQLLVDGRIWYSSGDCAF
                     NPIFGNYVNCLGIPRFDVERYYPGYPDAPHSGFLFTMDVGALLASGVAPGNHVLKVRV
                     GDQQQTFTELPNRDGISVFFECAEDRVGSALGFIDVPQTNDFMKGTVTFQGWALTEFS
                     ALTSVEIIVDGDFKGQAQYGFPRPDVVVQYPYLFNSQNSGWRFVIDTTKLGNSKHTLT
                     VRVLDSRGLRTEIGSTVFYTQNANPVP"
     gene            complement(6053..6682)
                     /locus_tag="VNN25_27750"
     CDS             complement(6053..6682)
                     /locus_tag="VNN25_27750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95402.1"
                     /translation="MAIVSVITFVLPTPARAVTLDRIAAVIDQQVLTVSEVDQMAEVR
                     FIPRIAGRSDDDYHHDILEALIAQALRYRDVERFGAQDIPKDSIEARLVEIQHRFAPP
                     DSLDAALLHAELTLDELRALIKRQLQVEAYIQERFAPLIFVANDDIAAYYNGPWRQQR
                     TARALPVPPLNDVREEIRTLIRSRQLEEQIATWTNQLRARANVDVYAWK"
     gene            7088..8161
                     /gene="hrcA"
                     /locus_tag="VNN25_27755"
     CDS             7088..8161
                     /gene="hrcA"
                     /locus_tag="VNN25_27755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXH95403.1"
                     /translation="MVQHIDRSGDDLDTRAREVLREIIMQHVATGEAISSRTLAKCGR
                     FQLSPASLRNVMADLEDLGYLQHPHTSAGRVPTDLGYRFFIDHLMRSRTLTQRERETI
                     DDQVSHANEIDEVLHLASRVLSRLSDQVGVIFMPTLLQFTVRSMDFVGVADNKIMCIL
                     VGQNGVVVNKIIETRMPFTRDELQKISRFITGEFSGMRLDSIRRRLIRMTEEERAMHD
                     GMLQKTITLGIEAVNDVAPLEHELYVEGTASILTKPEFADAVSMRKTFLALQEKEKLV
                     EILNSCLSEEGLQILIGSESNFTQIYNFSIVARRYGTQTAPLGMVGIIGPMRMEYARM
                     APLVDYLGLALSRKIEEEQEQQQ"
     gene            8158..8760
                     /gene="grpE"
                     /locus_tag="VNN25_27760"
     CDS             8158..8760
                     /gene="grpE"
                     /locus_tag="VNN25_27760"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXH95404.1"
                     /translation="MSEKNNPNDGDTLDDDRYVIEDTGDTVEDIEHEMEVAAQEAVDG
                     PAAPAAVPVADDFRDRYVRTLADFENFRKRSEREKNDFQRYALAGLIRDLLPVLDNFD
                     RGLDHAEEGDEFHKGMALIYKQLFDVLQRHGLKAITESGVRFDPNIHEGVVRVEDASV
                     PSHTVVEVMQKGYFLHDRLIRPAMVKVAVGGPDEAAPSGT"
     gene            8764..>10488
                     /gene="dnaK"
                     /locus_tag="VNN25_27765"
     CDS             8764..>10488
                     /gene="dnaK"
                     /locus_tag="VNN25_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXH95405.1"
                     /translation="MSKIIGIDLGTTNSCVAVVDVATPQVMANREGSRTTPSVVGFTE
                     DSERLVGQIAKRQAITNPFNTVFAIKRLIGRKYDTDEVSRAKQILPYQIVPADNGDMK
                     VKIRDREYSPEEISAFVLMEIKSFAEDFLGEPVTEAIITVPAYFNDSQRQATRDAGKI
                     AGLEVLRIINEPTAAALAYGLENKSNQRIAVYDLGGGTFDISILKLEDGLFEVLSTSG
                     DTYLGGEDFDKRIIDWLIEGFLKETGISLADDRMALQRLKEAAERAKHELSVAPESRI
                     TLPFISADASGPKHLNSVLTRSEFEKMVEDLIKRTIDPVNDALKEAGLTAADIDEVVL
                     VGGQTRMPRVVEAVQKVFGKEPNRNINPDEVVAVGAAVQAGILRGDIKDMILLDVTPL
                     SLGVETHGGVFTKVIERNSTIPTKNSLIFTTVADHQNKVTVHVLQGEREFVRDNKSLG
                     KFDLVGIPAAPRGVPQIEVTFSIDSNGIVSVTASDQATGQSTGVKINPAGGLSQDEID
                     RLVNEAAEHSRGDASRREIRMLQNKLEGMVYTNEKVFREFGKMLSDEDRDKVQKIITK
                     SKEQVTSEE"
BASE COUNT         1998 a         3281 c         3200 g         2009 t
ORIGIN      
        1 agatcggatt gttccccggc cggaactggg cgatgcgcca cgacgtgccc gcgcgcgcca
       61 tcattccgcg atgaagggcc gggcgcaggc cgcagttcac gagcgacgac ttccccgatc
      121 cggacgttcc gaccacgaca aggaaatgtg acgcggcaag cgtgtcgacc atggcatcga
      181 cctgcgattc gcgtccgaaa aagaggtgtt cttcttcctc ctggaatggc cgcaggccgg
      241 ggaacggatt cgtcgacatc acttcagtcc cagcgcctcc gtcaaagccg caatggcttg
      301 ctcctcggga gcgctcaacg cgtcgatgac gccggtctcg gtttgaagga gaaactgctt
      361 cttgctcgtc gcgggcccga cgatgtacgt gatcgtcttc ggcgggttgt cgcgacgtgc
      421 accttcgttc ttgcggatgt cactctgctg gtaggcgcgc cacgtgtcat cgccggcgcc
      481 ccagaagagc acgatcgcgc cggcggtcgc catgagctgt tcattagctt cacgcaccgc
      541 cgccgcgtcg ccgtcgaaaa cggtcagctc gactctcatc ccgcgggcga tgagactctt
      601 tgccagcggc acggtggctt gaatgtcacg agggtcgcag atgacgtaaa ccagcgtctt
      661 atcggtatcg gcaacgggag cggcggtagc acgctcgaca cgcgcaagtg ccgcacgcac
      721 ggcgctcttc agatcctcga tcccgccggt aatgaggtcg gcgccgaact gcaagccggc
      781 atcctgatgg agcgcggtga tgaaagtctg ctggcgcgga tcgctggtct gcaaatccgc
      841 cgggacggag atgacgcggg caagaccgtt cttccggctg atgttgaccg ccgattgatt
      901 ctgcagcgcc accaccgact gatcggttcc gtcagggacc gcaccgtagc tgctgccgat
      961 gagatggatc gatagttcgc actgctggag cagccgttcg acttcagcga cgtactccgc
     1021 cgagtcggtg gggagctggg aatcgggaac gatgtcgtat ccgaggcgct tcagctcgcc
     1081 ctcgagagtt gcgcgtgcgt cgcgctggtc acgcgtgcac tccgcgagat agatcgacgc
     1141 cctggccttg ccggccgcgg gcccgctcgc catcacgtcg agcaactcct tgatgtgcca
     1201 ggccacccgt gtaacggact tcagaaactc ctggcgtgcc gaatcgccat aagccgggtc
     1261 gagctcctgc ggcgtgttgt cggcgtcgag gtgatagaac tcgtagccga gaatgcgtcc
     1321 aagcactccc gccacttccg gcgggaatga ctcgtctttc ccgaacggtg tcttgatgat
     1381 tttgaagatg cggcatttgt tgccgacgat gatgccgccc gttttctccg cgatctcgta
     1441 aaactgagtc atctccttgc tgcaccagtc cgatttcaga tagcgcgggg agagaacgga
     1501 cacgagcaca gccgtctcgg ggaactgact gagaatctca tcggagaaga cgtcattccc
     1561 ctgcagcttc tcgtcccgcc agatgttcgc atccttgccc gtataccggc tgagcacctg
     1621 ccgaagcgcc ttgtcgaaga cggtcaccca cccttccttc tcatcaggaa gaggttcgtt
     1681 gtcgatatgg gcgtagctga tgaatgcgtc tcgcgtgaag ctcatggaag tcgccgtttc
     1741 ctgagggtga tggaggtatc gcagtataca gccggtgaac gagggcggat ttccctgcgg
     1801 agcgcaaggg ccttctcacg cgaagacgcg aagacgcgaa gggttgatgt ttgcgatgct
     1861 ctccttcgcg ccttcgcggc ttcgcgtgag cgtgccgtac ccttgcagga tcacttcttc
     1921 cgcgcgtcgc gcatcgcgtc cagtcgcgcc ttgatcttcg cctcggcgcc tgcttctttc
     1981 ggctcgtaga aacggcggcc ggccagtgcg tcgggaagac actccatcgc cgccgtctgg
     2041 tcttcgaagt cgtgggcgta ttgataaccg cggccgtagc cgaggtcttt catgaggcct
     2101 gtgggagcgt tgcggatgtg aaggggaaca cccgggttat cgccctgtcg cacttcgcga
     2161 acggcctcgc cgtagccgat gtagacggcg ttcgatttcg gtgcggcggc aagataggtg
     2221 accagttgcc ccagcgctac gccggcttcc ggcatgccga ggaagtgaac ggtttgctgg
     2281 acggcgatgg cgaagacgag cgcttgcgga tcggcgttgc cgatgtcttc cgatgcggcg
     2341 cgcacgaggc gacgggcgat gtacatcgga tcttcgcccc cttcgagcat gcgcaccagc
     2401 cagtacatcg aggcgtcggc gtcgccgttg cgaagcgatt tgtgaagcgc cgagatgatg
     2461 ttgtagtgct cttcgccgcc tttgtcgtac atcagcgaac ggcgctgaag gatctgcgtc
     2521 accttcgtct tgtcgatcgc gccgtcggcc ttcgttgccg tttcgacgac gagctgcagc
     2581 gagttgagcg cctggcgcgc gtcgcccgcc gaggtcgcgg ccatgaagtc gagagcatcg
     2641 tcgttgacgt cgatgccgta cttgcgcacc gagggatcgc tgacggcgcg gcggagaatc
     2701 gtcatgacct gttcgcgctc cagcgcgggg atgacgacga ctttcgtccg cgacagcagc
     2761 gccgagttca cttcgaacga cgggttctcg gtcgtggcgc cgatgaggat gatgtctccg
     2821 gcctcgacgt aggggaggaa ggcgtcctgc tgcgctttgt tgaagcgatg gatctcatcg
     2881 atgaagacga tggtcttcgt cccgcgcgct ttccgctcgc ggcgcgcttc ctccatcagt
     2941 gtgcggatct ccttgatggc agtgctggtg gccgagaacg tgacgaactt cgcccgggtc
     3001 ttgttggcga tgatgcgcgc cagcgtggtt ttcccggagc ctggcggccc ccagaagatc
     3061 atcgagggaa tccggtcgct ctcgattgcg gtgcggagga agcgtccctc gccgaggatc
     3121 tcgtcgaggc cgatgatctc ctcgagggag cgcgggcgca gccgctccgg cagcggcgca
     3181 tcgctgcgcg ctggcggttc gaaggggtcg tcagcgaaga gagaaccggt catggagtga
     3241 agagtaaaag ggatcgatca acagagcaga ctgaaatcga ggttgcctcc gctgaccacg
     3301 atcgcggtct ttcctttcgg tttgatcgcg cccgatagca gcgcggcaat tcccagcgcg
     3361 ccggtcggct cgatgacgag cttggttcga tacatcgcaa acttcacggc gtcgaggaga
     3421 acgccgtctt cgacgctgac gacgtcatga acccgttcgc ggatcacggc aaagttgcgc
     3481 tcgccgatag ccagcgtcct ggcgccgtcg gcgatcgtct tcggcggatc gatggtgacg
     3541 atcctgcccg acttgaacga ctgttcgcca tcgttgccgg cgcgtggctc gacgccgtag
     3601 accgcgatgt ccggcttgct cgacgtggcc gcgatcgaca cgcccgagag cagacctccg
     3661 ccgccgaggg gtgtaacgat tgtctccacg tccggccatt cgtcgacgat ttccagtccg
     3721 acggtgccgg cgccggcaat gatgcgccaa tcatcaaaag gagggatgac cgacgcgccg
     3781 ctcttgcttg cgatgtcgcg ggcaacagtg tcgcgcgtcg cggacgtcac gcccgaggtg
     3841 acgaccgtcg caccatacgc ttcggtcgcc gcaaccttcg acgggttgga tccctccggc
     3901 atcacgatgg tggccttcat gccgaggagt ctggcggcga gggcgacgcc ctgcgcgtga
     3961 ttgccggagg agaacgcgac gacgccgttg ccgcgctggt tctcaggcac ctgcaggagc
     4021 gcattcatcg ccccgcggat cttgaacgat ccggcgcgct ggaggttttc gcacttgaga
     4081 cgcacctcca cgccgatggc ttcggagagc gatcggctgc tcagaagggg ggtgcggtgg
     4141 acgtacggtt ggatacggag ggcggcttcc cggacatcct cgatcgtgac agcggacgca
     4201 tcgatatttg aagaattcac cgaggcagta taaactccgg caaactggcc atggattgaa
     4261 cgtcggttca cgaacgaacc ggcgcgatta cgcctcggct gagcagtatc gggcaaggtt
     4321 attttcgggg aggttggggt tatgtggaga agggttaccc gcggcctggt tctcgccatt
     4381 ctggtgttgt tcgttgtcgt gcccgtgctg accgcgcaaa cggtcgagac gcatggggcg
     4441 ctcgaatttc ctgactcgtc cgttacgcaa agcggcgtga tcctcgtcaa ggggtggttc
     4501 ctcgaccccg cgtcgatctc gaaggttgcc gtgtacgtcg acgatcagtt cgtggtcaac
     4561 tccaacacga atcttccgcg aatcgatgtg gagcagtcct acccgaacta tcccggcatc
     4621 cagcacatcg cgcccggctt tcagatcggc atgctagcca gccgtttcac gaatggagca
     4681 cacacgattg ccgtacgcgt gttcgccagc gacaacacga tcacggagct cggacgccgg
     4741 acgatctcgg tcgacaatac gatcaaccag tcgccattcg gcaatgtcga cattcccgac
     4801 cttgccgcca cgtacaacgc ctccggctcg ttcccggtct caggctgggc gctcgacacc
     4861 gacggcgtgg ccaaggtcga agtgctcatc gacaacggtg tcgtgcagaa cgccatgtac
     4921 ggcgatcccc gtccggatgt ggccaacacg atcgccgact ggcctgatgc gctttacagc
     4981 gcttttatcg ccaacgtcga cacgacgcga atcccggatg gcgtgcacac gctcgacgtg
     5041 cgcgcgaccg accgcctcgg cctctcgcgt ctcatcggcc gccgcaccgt gcagatcttc
     5101 aatgagggcg gaaacctgaa gccgttcggc tacgtcgatg agccgaagcc cgacgcggtg
     5161 ttgtacggca cgcgttgcaa cgtatttccc caggcctcac cggccatcaa tccgcaatcg
     5221 catatcacgc cggtccgcgg ctgggctctc gacctcggca cacgcgccga caccggccgc
     5281 gtcgcctacg ctcagctatt ggtcgacggc cgtatctggt attcgagcgg cgactgcgcc
     5341 ttcaatccga tcttcggcaa ttacgtgaac tgcctcggca tcccgcgttt cgacgtggag
     5401 cgttactacc ccggatatcc tgacgcgcca cactccggtt tcctcttcac gatggacgtc
     5461 ggcgcactgc tcgcgtccgg tgtggccccc ggcaatcacg tgctgaaagt gcgcgtcggc
     5521 gaccaacagc agacgttcac tgagctgccg aaccgcgatg gcatctccgt cttttttgag
     5581 tgcgcggagg accgcgtcgg ctccgccctg ggcttcattg atgtccccca gaccaacgac
     5641 ttcatgaagg ggacggtcac gttccagggc tgggctctga ccgaattcag cgcgctgacg
     5701 tcggttgaga tcatcgtcga cggagacttc aagggacagg cgcagtacgg cttcccgcgg
     5761 cccgatgtcg tggtgcagta tccgtatctg ttcaacagcc agaactcggg atggcgcttc
     5821 gtcatcgaca ccacgaagct cggcaactcg aagcacacac tgacggtcag ggttctggac
     5881 agccgcggcc tgcggacgga gataggttca accgtcttct acacgcagaa cgcgaacccg
     5941 gtaccttaga agacctggtc accacagagg cacagaggtc acagagtatg aattcgactc
     6001 tgtgtcctca gtgtctctgt ggttcatttg tcgtgcgacg cattccaaga agtcacttcc
     6061 acgcgtacac atccacattc gcacgcgctc gcaactgatt cgtccatgtt gcgatctgct
     6121 cttcgagctg gcgtgaccgg atgagcgtgc gaatttcttc gcggacgtcg ttgagcggcg
     6181 gcaccggaag tgctctcgcg gtacgttgct gacgccacgg accgttgtag tacgcggcga
     6241 tgtcgtcgtt ggcgacgaag atcagcgggg cgaagcgttc ctggatgtac gcctccacct
     6301 gcaactggcg cttgatgagt gcgcgcaact cgtcgagagt caattccgcg tggaggagcg
     6361 ctgcgtcgag cgaatccggc ggtgcgaaac ggtgctggat ctccaccagc cgcgcttcga
     6421 tcgagtcttt ggggatgtcc tgcgctccga agcgttccac gtcgcgatac cgcagtgcct
     6481 gcgcgatcag agcttcgagg atgtcgtggt ggtaatcgtc gtcgcttcgt ccggcaatgc
     6541 gcggaatgaa gcgaacctcg gccatctggt cgacttcgct gacggtcaga acctgttgat
     6601 cgatgacggc cgcgatgcgg tcgagagtca cggcgcgcgc gggcgtgggc agaacgaagg
     6661 ttatgacaga gacgatcgcc acgccgagtg attggatccg ccgccggacg tgatgtgtca
     6721 tcctgagcct gccggaggcg cccgcgctaa ccgctactct tgcgctgacc ttgctcggcg
     6781 ccgcaggacg gcgaaggatc tcaagttgcc aggcacacgg catgtgcgtc ctaagttgag
     6841 atccttcgcc gtccttcgcc gccaagccgt ctgtctggcg cgccgtgtga gtacgaatgg
     6901 cggctccggc tgtctcagga tgacacatca attgagctcc cctccttcaa cgtcaacctt
     6961 cctttcatcg ctccgcaaat cttgacacca tccggcgcaa ttcctacatt ctcgtgcttg
     7021 ttggcagtca ttcggcgaga ctgccagaca agcccggcca ttcgctgcca aactcgcaat
     7081 taaactcatg gtccagcata tcgatcgaag cggtgatgat ctcgacacgc gcgcccgcga
     7141 ggtgctgcgc gagatcatca tgcaacacgt ggccacgggt gaggccatca gctcgcgcac
     7201 cctggcgaag tgcgggcggt ttcagctctc gccggcaagc ctgcgcaacg tgatggcgga
     7261 tctcgaggat ctcggctacc tccagcatcc tcacacctca gccggccgcg tgccgacgga
     7321 ccttggctac cgtttcttca tcgaccatct catgcgctcg cgcacgctca cgcagcgcga
     7381 gcgcgagacg atcgatgacc aggtcagtca cgccaacgag attgacgaag tgcttcatct
     7441 cgcctctcgc gttctctcca ggctcagcga ccaggtcggc gtcatcttca tgccgacgtt
     7501 gctgcaattc acggtccgca gcatggactt cgtcggtgtc gccgacaaca agatcatgtg
     7561 catcctcgtc gggcagaacg gcgtggtcgt gaacaagatc atcgagacgc ggatgccgtt
     7621 cacgcgcgac gaacttcaga aaatctcgcg cttcatcacg ggcgagttca gcgggatgcg
     7681 gcttgacagc attcgccgtc gtctgattcg gatgaccgag gaagagcggg ccatgcacga
     7741 cggcatgctg cagaagacca tcaccctcgg catcgaagcc gtcaacgacg ttgcgccgct
     7801 cgagcacgag ctctacgtcg aaggaacggc ttcgatcctc accaaacccg agttcgccga
     7861 cgccgtctcc atgcgcaaga ccttcctggc cctgcaggaa aaagagaagc tggtggagat
     7921 cttgaactcc tgtctctccg aagaaggtct gcagatcctg atcggatcgg agagcaattt
     7981 cacgcagatc tacaacttca gcattgtggc gcggcgctac ggcacgcaga cggctccgct
     8041 cggcatggtc ggcatcatcg gaccgatgcg catggagtac gctcgcatgg cgccgctggt
     8101 cgactacctc ggtctcgcgc tcagccgcaa gatcgaagaa gaacaggagc aacagcagtg
     8161 agtgaaaaga acaacccgaa tgatggtgac accctggacg acgaccgcta cgtgatcgaa
     8221 gacaccggcg acaccgtcga ggacatcgag cacgaaatgg aagtggcggc ccaggaagcg
     8281 gttgatggtc cggcagcgcc ggccgccgta cccgttgcgg acgactttcg cgaccgctac
     8341 gtccgcacgc tcgccgactt cgagaacttt cgcaaacgct ccgagcgtga gaagaacgac
     8401 ttccagcgct acgccctggc agggttgatt cgcgatcttc tgccggtgct cgacaacttc
     8461 gaccgcggac tcgaccacgc ggaagagggc gacgagtttc acaagggcat ggccctcatc
     8521 tacaagcagc tcttcgacgt cctccagcgg cacggcctga aggcgatcac ggagtccggc
     8581 gtccgcttcg atccgaacat ccatgaagga gtggttcgcg tagaggacgc atccgtcccc
     8641 tcgcacaccg tcgttgaggt catgcagaag ggttactttc tccacgatcg tttgattcgc
     8701 ccggcaatgg tcaaggttgc ggtcggtggt ccggacgagg ctgcgccgtc aggcacgtaa
     8761 ttcatgtcga agatcatcgg aatcgatctc gggacgacga actcctgtgt tgccgtcgtc
     8821 gacgtcgcca cgccgcaggt gatggccaac cgggaaggca gccgcaccac tccttcggtc
     8881 gtcggattca ccgaagacag cgaacgtctg gtcggccaga tcgccaagcg ccaggccatc
     8941 acgaatccgt tcaacacggt tttcgccatc aagcgcctga tcggccgcaa gtacgacacg
     9001 gatgaggtca gccgcgccaa acagatcctc ccgtatcaga tcgttcccgc cgacaacggc
     9061 gacatgaaag tgaagatccg cgaccgcgaa tactcgccgg aagagatctc cgcattcgtt
     9121 ctcatggaga tcaaatcatt tgccgaggac tttctcggcg agccggtcac ggaggccatc
     9181 atcaccgtgc cggcgtactt caacgacagc cagcgccagg ccacgcgcga cgccggcaag
     9241 atcgccggac tggaagtgct tcgcatcatc aacgagccga cggccgcggc gctcgcgtat
     9301 ggtctggaaa acaagtcgaa ccagcgcatc gccgtctacg acctcggcgg cggcaccttc
     9361 gacatctcga ttctcaaact cgaggatggt ctcttcgaag tcctctccac gtccggcgat
     9421 acctacctcg gcggtgaaga cttcgacaag cgcatcatcg actggctcat cgaaggcttc
     9481 ctgaaggaaa ccggcatcag tctcgccgac gaccggatgg ccctgcagcg tttgaaggaa
     9541 gccgccgagc gcgccaagca cgagctttcc gtcgctcccg aatcgcgcat cacgcttccg
     9601 ttcatctccg ccgacgcctc ggggccgaag catctgaaca gcgtcctcac ccgaagcgag
     9661 ttcgagaaaa tggtcgagga cctgatcaag cggacgatcg atcccgtcaa cgacgcgctc
     9721 aaagaagccg ggctcaccgc cgccgacatc gatgaagtcg tcctcgtcgg cggtcaaacg
     9781 cgcatgcccc gcgtcgtcga ggccgtgcag aaagtcttcg gcaaagagcc caaccgcaac
     9841 atcaatcctg atgaagtcgt cgcggtcggc gccgccgttc aggccggtat tctgcgcggc
     9901 gacatcaagg acatgatcct gctcgatgtc accccactgt cgctcggtgt cgaaactcac
     9961 ggcggcgtct tcaccaaagt gatcgagcgc aactcgacga tcccgaccaa gaactcgctg
    10021 atcttcacca cggtcgccga tcatcagaac aaagtcaccg tgcacgtgct ccagggcgag
    10081 cgcgaattcg tgcgcgacaa caaatcgctc ggtaagttcg acctcgtcgg catcccggcg
    10141 gcgccgcgtg gcgtgccgca gatcgaagtc accttttcga tcgactccaa cggcatcgtc
    10201 agcgtcacgg ccagcgatca ggccaccggc cagtcgaccg gcgtgaagat caatccggcc
    10261 ggcggtctgt cgcaggacga gatcgaccga ctggtcaacg aagctgcaga acactcccgc
    10321 ggcgacgcct cgcgccgcga gatccgtatg ttgcagaaca agctcgaggg catggtttac
    10381 acgaacgaga aggtcttccg cgagtttggc aaaatgctca gcgacgaaga ccgcgacaaa
    10441 gtccagaaga tcatcaccaa atcgaaagag caggtgacct cggaagaa
//
LOCUS       DATLVW010000938         9522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_39220, whole genome shotgun sequence.
ACCESSION   DATLVW010000938 DATLVW010000000
VERSION     DATLVW010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39220"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VNN25_27770"
     CDS             <1..408
                     /locus_tag="VNN25_27770"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HXH95406.1"
                     /translation="HVKPHGALYNDAHKDRTLAEIVIDAIRAVDPSLPIVCSDSSKMA
                     AAARAASTPVIREAFADRRYNADGSLVARSEPGSLLDVAEAAAQAALLANEGAVIARD
                     GTHITIPFDTICIHADMKHAVERLRAIRAKITR"
     gene            complement(398..1048)
                     /locus_tag="VNN25_27775"
     CDS             complement(398..1048)
                     /locus_tag="VNN25_27775"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXH95407.1"
                     /translation="MHAGRTAFVIDGFNVYHSLVDASRSLGLPDESGTKWLNLRALCA
                     SYLPVFGVDANLAGVHYFSALAHHLQATHPGLTARHQLYVRVLKETGVSVELGRFKKR
                     PITCRACRKTIKCHEEKETDVAVASKLFEILALDLADRVVLVTGDTDLAPAVRTVKKL
                     YPDKEVCFAFPWNRKQAELEALVTTSISITREAYLRHQFSNPAVIGRKSFRKPVTW"
     gene            1278..3674
                     /locus_tag="VNN25_27780"
     CDS             1278..3674
                     /locus_tag="VNN25_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXH95408.1"
                     /translation="MKRIAAALLFAIVALAAAAQQFDLSIDNIMRGTGLYGWTPEEVR
                     WSPDGQHVYFSWKLYTDTLEKDRDTYVVNRDGSGLRKLSDEEKKDAPPANAQWTRDKH
                     RAVYTDEGDVFLWDAATNKRRALTQTNENESSPQFTFDETRVTFVRANNVYAIDLANG
                     SLTQLTNVVAPDDKGPNVTLWDDAAKKGTASQEYIKGEERKLLDIIDRKAKKREEDEA
                     KRKREHPIKPFKMDKKQTVATARLTPDGKYVVLVVNNESDKAKKTIVPNYITEAVYTD
                     TIPGREKVGDAQPPSRIAVVSSTTGEVKWFDHGLKPLTSPEKESTTATKATTEDQGKA
                     SEQKTEKSADTQKESTQPKEREVNLRAPIWSEDGKHAFVVVRSSDNKDAWIMAFDPAS
                     AKGRILVTMHDDAWLRFDIAFGWLADNATIYYLSEATGFMHLYEVGFDGGTAKALTSG
                     KWEVDSVSLSDDKKSFYLTTSEGSPFERHLYRMPVTGGPSTKLTSMPGDNEAEVSRDG
                     GAIANVYSYTNKPPELYIGSKRVTTSPAPDFAKQAWLDVPIVDVPARDGVNVPARIYK
                     PANWKRGGKAVVFVHGAGYLQNVHRWWSSYSHEYMFHHLLMTKGYLVLDVDYRGSRGY
                     GRDWRTAIYRHMGGKDLDDQVDAARWLVKEHGVDPKRIGVYGGSYGGFITLMAMFTTP
                     DVFAAGAALRPVTDWAQYNNGYTSGILNFPQTDAEAYRKSSPIYFAQGLKGKLLICHG
                     MVDTNVHFQDSVRLAQRLIELRKTNWELAPYPVENHGFVEPTSWADEYKRILKLFDTM
                     "
     gene            3840..6314
                     /locus_tag="VNN25_27785"
     CDS             3840..6314
                     /locus_tag="VNN25_27785"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HXH95409.1"
                     /translation="MNPVASPPEPAPAHAAANLREGIETFLRFARGKVWQDASRHEKL
                     HALSIAVRRPMLDAMRDTERRYRDQESKRLYYFSMEFLMGRALGNNLTNLNLYDEAKA
                     TIAELGVDFDEIQELEHDAALGNGGLGRLAACFLDSLASLDMPGFGYGINYEFGLFRQ
                     TFMNGYQHEKPDHWLEQDGSPWLIDRAGESITVPLYGSIAHTTIDGVYKPKWVGFKEI
                     VGVPHDMPIVGYGGRTVNVLRLYTARSSDEFDIGIFNSGDYIAAVQHKINTEAISKIL
                     YPSDSVDRGRELRLTQEYFLVACSVRDIVKRYLKTHSTFDEFAEKVAIQMNDTHPALT
                     VAELMRLFIDDYGLPWEQAWSITVATCGYTNHTLMPEALEKWPFDLIERLLPRHLQIV
                     QEINRRLLAEVERRFPGDATMVQRVSLFDDGERRNVRMGNLAMAGSHSINGVAALHSE
                     LVKTALAPEFHKLYPERFNNKTNGVTPRRWLLHANRPLASLITRAIGDSWIRNLDDLR
                     QLEGFVDDAGLLDRLDSVKSRNKLALAHLTKKMTNIVIDPSSLFDVQVKRMHEYKRQL
                     LNILHVVDRYWQLAEDRQTPLVPRTFFFAGKSAPGYFMAKLIIKLIHSVGEVVNADPR
                     TRDWLRVVFLPDYRVTLAEAIIPAAELSEQISTAGKEASGTGNMKLALNGALTIGTLD
                     GANIEIRDEVGEEYIFIFGLRADEVARLVADGYHPEHYLGSSRDLRRVVDSIASGHFS
                     RGDKDIFRPIVANLLSARDEYVHLADLEPYIEAQQRVDITWRDRAQWNRKSLLNIARM
                     GKFSSDRTIGEYAREIWNIKAAGVGR"
     gene            6671..8119
                     /locus_tag="VNN25_27790"
     CDS             6671..8119
                     /locus_tag="VNN25_27790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXH95410.1"
                     /translation="MVRFRDLSIRSKLMTGFMLTSLAALLLTIGALAAYDGSTFRGEV
                     LHDVSTLAGIIGENASSSVAFNDKESAKASLAALRAQPQITGAAIYDSNGALFATYTR
                     AKDGDTPGALPRDGEWIDAQHIGVTRPVLFNGARVGTVTVRSDLSELAIRRTRTLQIA
                     GGIYVGAAFLALLLASLFQRAITRPIHRLLRAEKRVSREKDYTLRVEKEANDEIGLLI
                     DGFNEMLSEIRARDAEILAKHDQEMALARAIQTSVLPRTFELPGYDISAIMLPAEEVG
                     GDFYEFRCTGNGAWIGVGDVTGHGVTSGLIMMMAQAMFTMLCEQNGHYQSPAKFVSLL
                     NRAMFYNLKSRLGQEKFMTMVVARIEGDGKIVYAGAHTDLLVYRAADKSVERLPTEGL
                     WLGISADVESHTIDRSISLRRGDVALLHTDGVTESRNTAGECYDIDRLTAELKRLHDE
                     PAETIVSSIAATAWQWAGTPKDDVSLMAVKRS"
     gene            8123..8605
                     /locus_tag="VNN25_27795"
     CDS             8123..8605
                     /locus_tag="VNN25_27795"
                     /inference="COORDINATES: protein motif:HMM:NF039711.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HXH95411.1"
                     /translation="MITERLSSDYVEMRFGPRWTYISCVRKFVASFFMIGLSDKERAE
                     QISMAASELLENAVKYASEEEGYLKISIAKAENEVDVCVKNKAEPHHINTLRRELALI
                     QTGQPEQVYLKKMEEAAMTEGQSRLGLIRILYEAGARLRLDVNDNEVSIHAIFKLEAA
                     "
     gene            8602..8991
                     /locus_tag="VNN25_27800"
     CDS             8602..8991
                     /locus_tag="VNN25_27800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95412.1"
                     /translation="MSAERGFVPLTVESLSIVPRLDGDTLRIAMSGAVEMRDPGDALN
                     PYWTLIDEEARRRNMKVVELDVQDLNFMNSSGILTLVRWITRCKSTPDCYRLVLRYDR
                     NVTWQRASIPTLAKLAPNVVVPMEIDA"
     gene            8984..>9522
                     /locus_tag="VNN25_27805"
     CDS             8984..>9522
                     /locus_tag="VNN25_27805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HXH95413.1"
                     /translation="MPEVERAPGYDLTCHACGAAVRPIDQFCSQCGRRDPAHLQGVQS
                     HDGSLTSTTLVSDGLTNEDTYPTFVAPEESMGQGTLIKSVRREHSTRLNTLAVETMLA
                     PGTVFARRYRIQRFLGSGAMGYVCCAIDESIDEVVALKVLSLPIHEDPDAYDRFKTEL
                     KLARRIRHRNVVQSFDLGFA"
BASE COUNT         1960 a         2978 c         2894 g         1690 t
ORIGIN      
        1 cacgtcaaac cccacggcgc gctctacaac gacgcgcaca aagatcgaac gctcgcagag
       61 atcgtgatcg atgcgattcg cgcagtcgat ccatcgctcc cgatcgtctg cagcgattcc
      121 tcgaagatgg ccgccgccgc gcgcgccgcc agcacacccg tgatccgcga ggccttcgcc
      181 gatcgccgct acaacgctga cggctccctc gtcgcacgca gcgagcccgg ttcgctgctc
      241 gatgtcgccg aagccgcagc gcaggcggct ctactcgcaa acgaaggggc cgtcatcgcc
      301 cgcgacggca cgcacatcac catcccattc gacacgatct gcattcatgc cgatatgaag
      361 cacgccgtcg agaggttacg ggcaatacga gcgaagatca ccaggtgacc ggcttgcgga
      421 acgacttcct gcctatgaca gcgggatttg agaactgatg acgtagatag gcctcgcgag
      481 taatgctgat ggaggtcgtc acgagagctt ccaactccgc ctgtttcctg ttccacggga
      541 acgcgaagca cacttccttg tctggataga gcttcttcac ggttcgaacg gctggggcga
      601 gatccgtgtc tccggtcacc agaacaacgc ggtcggcgag atcaagagca aggatctcga
      661 agagcttgga cgccacggca acgtctgtct ctttctcctc atgacatttg atcgtctttc
      721 gacaggcacg gcaggtaatc ggcctctttt tgaatcgacc cagttcaact gagacgcccg
      781 tctccttcag aactcgaacg tacagttgat ggcgtgcggt gagtcccgga tgtgttgcct
      841 gaagatgatg ggcaagcgct gagaaataat gaactccggc cagattggca tccactccga
      901 ataccggcaa gtacgacgca cacaaggcac gcaggtttag ccacttggtt ccgctctcgt
      961 ccggtaaacc gagacttctg ctcgcatcta ccagcgagtg gtagacgttg aagccatcga
     1021 tcacgaacgc tgtgcgacct gcgtgcataa aaaaaaacct ccggatcgta acccggaggg
     1081 ggccagcgaa ccctaccgat accctgctag gcgtgtcgct ggtggggacg cttttaatat
     1141 aagtactacc cagaggtcag tcaagaagat agtggctgca tgccgtaact gttataccaa
     1201 tgagaccgat tccccgcaca agcaacgcat caaagtcgga acccgaagca acggttccct
     1261 ccggtacgat acgcggcatg aaacgcatcg ccgccgctct ccttttcgct atcgtcgcgc
     1321 tcgccgcagc cgcgcagcag ttcgatctct cgatcgacaa catcatgcga gggaccgggc
     1381 tttacggctg gacgcccgag gaggtgcggt ggtcgccgga tgggcagcac gtctactttt
     1441 cgtggaagct ctacaccgac acgctggaaa aggatcgcga cacctacgtc gtcaaccgcg
     1501 acggcagcgg gctgcgcaaa ctcagcgatg aggagaagaa ggacgccccg ccggcgaatg
     1561 cgcagtggac gcgcgacaag catcgcgctg tttacaccga tgaaggcgac gtctttctct
     1621 gggatgcggc aacgaacaag cgccgcgcac tgacgcagac caacgagaac gagagctcgc
     1681 cgcagttcac cttcgatgag acgcgcgtga cgttcgtccg ggcgaacaac gtctacgcta
     1741 tcgacctcgc gaacggatcg ctgacgcagc tcacgaacgt tgtcgcgccc gacgacaagg
     1801 ggccgaatgt cacgttgtgg gacgacgccg cgaagaaggg aacggccagt caggagtaca
     1861 tcaaaggcga ggagcgcaag ctgctcgaca tcatcgaccg caaggccaag aagcgcgaag
     1921 aggacgaggc caagcggaag cgcgaacatc ccatcaaacc gttcaagatg gacaagaaac
     1981 agactgtcgc cactgcgcgg ctcactccgg acggcaagta cgtcgtcctc gtcgtcaaca
     2041 acgagtccga caaagcgaaa aagacgatcg tgccgaatta catcaccgag gcggtgtaca
     2101 cggacacgat tcccggacgc gagaaagtgg gggatgccca gccgccgtcg cgcatcgctg
     2161 tggtcagttc aacgactggc gaggtcaagt ggttcgatca cggactcaag ccgctgacgt
     2221 cgcccgagaa agaatcgacg actgctacga aggctacgac cgaagatcag ggaaaggcgt
     2281 ccgagcagaa gactgaaaag agcgccgaca cgcagaagga gagcacgcag ccgaaggagc
     2341 gcgaggtcaa cctgcgtgct ccgatctggt ctgaggacgg caagcacgcc ttcgtcgtcg
     2401 tccgttcgtc ggacaacaaa gacgcctgga tcatggcttt cgatccggcc tcggcgaagg
     2461 gacgaatcct cgttacgatg cacgacgacg cctggctgcg cttcgacatc gcgtttggct
     2521 ggctggccga caacgccacg atttactacc tctccgaggc caccggcttc atgcatctgt
     2581 acgaagttgg attcgacggc ggcaccgcga aggcgctcac gagcggcaag tgggaagtcg
     2641 acagcgtgag tttgtcagac gataagaagt cgttctacct cacgaccagc gaagggagtc
     2701 cgttcgagcg ccacctctac cggatgccgg tcactggcgg accctcgacg aagctgacgt
     2761 cgatgcccgg cgacaacgag gcagaagttt cgcgcgatgg aggcgccatc gccaacgtct
     2821 actcgtacac gaacaagccg cccgagctgt acatcggttc gaagcgcgtc acgacttcgc
     2881 ctgcgcccga cttcgcgaag caggcatggc tcgacgtccc gatcgtcgac gttcctgcgc
     2941 gcgacggcgt gaacgtaccg gcccgcatct acaagccggc gaactggaag aggggcggca
     3001 aagcagtggt cttcgtgcac ggcgccggct atctgcagaa cgttcatcgc tggtggtcga
     3061 gctactcgca cgagtacatg tttcatcact tgctgatgac gaagggctac ctcgtgctcg
     3121 acgtcgatta ccgcggttcg cgcggctacg ggcgcgactg gcgcacggcc atctaccggc
     3181 atatgggcgg caaggatctc gacgatcagg tcgacgccgc gcgctggctc gtgaaggagc
     3241 acggcgtcga tccgaaacga atcggcgtct acggcggcag ttacggcggc ttcatcacgc
     3301 tgatggcgat gttcacgacg ccggatgtct tcgccgccgg cgccgcgctg cgtcccgtca
     3361 ccgactgggc ccaatacaac aacggctaca cctcgggcat cctcaacttc ccgcagaccg
     3421 acgccgaggc gtatcgcaaa tcgtcgccga tctacttcgc gcagggactc aaaggaaagc
     3481 tgttgatctg ccacggcatg gtcgacacga acgtgcactt ccaggacagc gtccgcttgg
     3541 cgcaacgcct gatcgaactg cgcaaaacga actgggagct ggctccatac ccggtcgaga
     3601 accacggctt cgttgagccg acgagctggg cggatgagta caagaggatt ttgaagctgt
     3661 tcgacacgat gtagcgcttc gcgcgttgtc ggttgtcggt tgattgcgct tcgcgcgttg
     3721 tcaggggatg aaccggcctc gcatggcacg tgagcgacca tcggcagctg acaacgcgcg
     3781 aagcgcaatc aaccgacaac cgacaacgcg cggccgtcag gccgcgcaat aatgtcccta
     3841 tgaatcccgt cgcctcccca cccgaacccg ctcccgccca cgccgccgcc aatctgcgcg
     3901 aaggcatcga gacctttctt cgattcgcgc ggggcaaggt gtggcaggac gcatcgaggc
     3961 acgagaagct tcacgccctt tccattgcgg tccgccgtcc gatgctcgac gccatgcgcg
     4021 ataccgaacg gcgctatcgc gatcaggagt cgaagcgcct ctactacttt tcgatggagt
     4081 tcctcatggg ccgtgccctc gggaacaacc tgaccaacct gaatctctac gacgaggcga
     4141 aggcgacgat cgccgagctt ggtgtcgact tcgacgagat ccaggagctc gagcacgacg
     4201 ctgctctcgg caacggcggc ctcgggcggc tggccgcgtg cttcctcgat tcgctagcat
     4261 cgctcgacat gcccggcttc ggctacggca tcaactacga gttcggtctc ttccgtcaga
     4321 cgttcatgaa tggctatcag catgagaagc ccgatcactg gctcgagcag gacggctcgc
     4381 cgtggctcat cgatcgggcc ggcgaatcga tcacggttcc gctctacggc tccattgcgc
     4441 acacaacgat cgacggcgtt tacaaaccga agtgggtcgg gttcaaagag atcgtcggcg
     4501 ttccgcacga catgcccatc gtcggctacg gcgggcggac ggtgaacgtg ctccgtctct
     4561 acactgcccg ctcttccgac gagttcgaca tcggcatctt caactccggc gactacatcg
     4621 cggcggtgca gcacaagatc aacaccgagg ccatctcgaa gatcctctat ccctcggatt
     4681 ccgtcgatcg gggccgtgaa ctgcgcttga cgcaggagta ttttctcgtt gcctgttccg
     4741 tgcgcgacat cgtgaagcgc tatctgaaga cgcacagcac cttcgatgag tttgcggaga
     4801 aggtggcaat ccagatgaac gacacgcacc cagcgctcac ggtcgccgaa ctgatgcgcc
     4861 tcttcatcga cgattacggg ctgccatggg agcaggcgtg gtcgatcacg gtggcgacgt
     4921 gcggatacac gaatcacacg ctgatgccgg aggcgctgga gaagtggcca ttcgacctga
     4981 tcgagcgcct gctgccgcgt catctgcaga ttgtgcagga gatcaatcga cgtctgttgg
     5041 ctgaggtgga gcggcgcttt cccggcgacg cgaccatggt gcagcgcgtg tcgcttttcg
     5101 acgacggcga gcgccgcaat gtgcggatgg ggaacctggc gatggccggc agccattcga
     5161 tcaacggagt tgcggctctg cactccgagc tcgtcaagac ggcgctggcc ccggagtttc
     5221 acaagctcta tcccgagcgt ttcaacaaca agacgaacgg cgttacgccg cggcgctggc
     5281 tgctgcacgc caaccggccg ctggcgtcgc tcatcacgcg ggcgatcggc gatagttgga
     5341 tccgcaatct agacgacttg cggcagctcg aaggattcgt cgatgacgcc ggcctgctcg
     5401 accgcctcga ctcggtcaag agccggaaca agctcgccct cgcgcacctg acgaagaaga
     5461 tgaccaacat cgtcatcgat ccgagctcgc tgttcgatgt gcaggtcaag cggatgcacg
     5521 agtacaagcg ccaactgctg aacatcctgc acgtcgtcga ccgctattgg cagctcgccg
     5581 aagaccgtca aacgccgctc gtgccgcgca cgttcttctt cgcgggcaag tcggcgcccg
     5641 gctatttcat ggccaagctg atcatcaagc tgattcactc cgtcggcgag gttgtgaacg
     5701 ccgacccgcg aacgcgcgac tggctgcgcg tcgtgtttct tcctgattac cgcgtcacgt
     5761 tggccgaggc gatcattccg gcggcggagc tgagcgagca gatctcgacc gcgggaaaag
     5821 aagcctcggg taccgggaac atgaagctcg cgctcaacgg cgcgctgacg atcggtacgc
     5881 tggacggagc gaacatcgag atccgcgacg aggtgggcga ggagtacatc ttcatcttcg
     5941 gccttcgcgc ggatgaggtg gcgcgccttg ttgccgatgg ctatcacccc gagcactacc
     6001 tcggctccag ccgcgacctg cgccgcgtcg tcgactccat tgcctcgggg catttctccc
     6061 gcggcgacaa agacatcttc cgccccatcg tcgccaacct gctcagtgcc cgcgacgagt
     6121 acgtacatct cgccgacctc gagccgtaca tcgaggcgca gcagcgagtc gatatcacct
     6181 ggcgcgatcg cgcgcagtgg aaccgcaagt cgctcctgaa catcgcgcga atgggcaagt
     6241 tctcctcgga tcgcacgatc ggcgagtacg cgcgcgagat ctggaacatc aaggccgcgg
     6301 gcgtcggtcg ataacttacg acctccccaa tcgtgtcatc ctgagcctgc cggagccgcc
     6361 cccgctgctt gatcccttcg cggcgcgtga agtggcggcg aaggacggcg aaggatctcc
     6421 ggttgcgagg catgtggctt gtgcgtcgta gtttgagatc cttcgccgtc ctacgccgcc
     6481 tcatcgagcc gcgcaaattg atcgagcatg gaggcggctc cggcagtctc aggatgacac
     6541 cgtggatgaa gccggatgac acttcgatag aaccttcgct gacaccaaac ccgacgccgt
     6601 aaagaacgca cgaagccctg tcgaagcgtt gattcctacc cgcccgcgat agacttagcc
     6661 actcacacaa atggtccgtt ttcgcgatct ctccattcgc agcaagttga tgaccggctt
     6721 catgctgacg agtctggcag cgctgctgct gacgatcggc gcgctggctg cgtacgacgg
     6781 ctcgacgttc cgcggcgaag tgctgcacga cgtcagcacg ctcgccggca tcatcggcga
     6841 gaacgcgtcg tcatcggttg cgttcaacga caaagagagc gcgaaagcat cgctcgcggc
     6901 gctgcgggcg cagccgcaga ttaccggcgc cgcgatctac gacagcaacg gcgctctgtt
     6961 cgcgacgtac acccgcgcaa aagacggcga cacgccgggc gcattgccgc gcgatggcga
     7021 gtggatcgat gcccagcaca tcggcgttac gcggccagtg ctctttaatg gcgcacgcgt
     7081 cgggacagtc accgtccgtt ccgatctgag tgagctggcg atacgaagga cgcgaacgct
     7141 gcagatcgcc ggcggcatct acgtcggcgc tgccttcctc gcgctccttc tcgcatcgct
     7201 cttccagcgc gccatcacgc gtccgattca ccggttgctg cgcgcagaga agcgcgtctc
     7261 ccgcgagaag gactacaccc tgcgcgtcga gaaggaagcg aacgatgaga tcggactcct
     7321 catcgacgga ttcaacgaga tgctctccga gatccgcgcg cgcgacgcgg agatcctggc
     7381 gaagcacgat caggagatgg cgctggcgcg tgccatccag acgtcggtcc tgccgcggac
     7441 gttcgagctg cccggctacg acatctcggc gatcatgctg cctgccgaag aggtcggcgg
     7501 cgacttttat gagttccgct gcacgggcaa cggcgcatgg attggtgtcg gcgacgttac
     7561 cggccacggc gtgacttcgg gcctgatcat gatgatggcg caggcgatgt tcacgatgct
     7621 ttgcgagcag aatgggcact accagtcgcc cgcaaaattc gtctcgcttc tgaatcgcgc
     7681 catgttctac aacctcaagt cacgactcgg ccaggagaag ttcatgacga tggtcgtggc
     7741 gcgcatcgaa ggcgacggca agatcgtcta cgccggtgcg cacaccgatc tgctcgtcta
     7801 tcgtgccgcg gacaagagcg tcgagagatt gccgaccgag gggttgtggc tcggcatctc
     7861 tgccgatgtc gagtcgcaca cgatcgacag gtcgatctct ctccgccggg gcgacgtggc
     7921 attgcttcac accgacggag tgaccgaatc gcgcaacaca gccggcgagt gctacgacat
     7981 cgaccggctg accgcggaac tgaagagact tcacgatgag cccgcggaga cgatcgtctc
     8041 atcgatcgcc gcgacggcat ggcagtgggc cgggacgccg aaggatgacg tcagcctgat
     8101 ggcagtcaaa aggagctgaa gaatgatcac ggagcggcta tcttccgact acgtcgagat
     8161 gcggttcggg ccgcgatgga cgtacatcag ttgcgtgcgc aagttcgtgg cgagcttttt
     8221 catgatcgga ctctcggaca aggaacgcgc cgagcagatc tcgatggctg cctccgagct
     8281 cctcgaaaac gccgtcaagt acgcctcgga agaggagggc tacctcaaaa tctcgatcgc
     8341 caaggccgag aacgaggtcg acgtctgcgt gaagaacaag gctgagccgc atcacatcaa
     8401 cacgctgcgc cgcgagctcg ccctcattca gaccggtcag ccggagcagg tctatctgaa
     8461 gaaaatggag gaggcggcga tgaccgaggg gcagagccgg ctcggactga tccggatttt
     8521 gtacgaggcg ggcgctcgat tgcgtctcga cgtcaacgac aacgaggtct ccattcacgc
     8581 catcttcaag ctggaggcag catgagcgcc gagcgtggtt tcgttcctct cacggtcgaa
     8641 tcgctctcga tcgtcccccg gctcgacggc gatacgctcc gcatcgccat gagcggcgcg
     8701 gtggagatgc gcgatcccgg cgatgcgttg aatccttact ggactctgat cgacgaagaa
     8761 gcgcgccgcc gcaacatgaa agtggtcgag ctcgatgttc aggatctcaa cttcatgaac
     8821 agctcgggca tcctcacgct cgtccgctgg atcacgcgct gcaaatcgac acccgattgc
     8881 tatcgtctcg tgttgcgcta tgaccgcaac gtaacctggc agcgagcttc catccccacg
     8941 ttggccaagc tcgctccgaa tgtcgtcgta ccgatggaga tcgatgcctg aagttgaacg
     9001 cgcgcctggt tacgacctga cgtgccatgc ctgtggcgcc gcagtgcggc ccatcgacca
     9061 gttctgcagc cagtgcggac ggcgcgaccc tgcgcacttg cagggtgtgc agagccacga
     9121 cggcagtctg acgtcgacca cgctcgtgag cgacggtctc accaacgaag acacgtaccc
     9181 gacattcgtc gcgcccgagg agtcgatggg gcaggggacg ttgatcaagt cggtacggcg
     9241 cgagcactcg acgcgtctga acacactcgc ggtggagacg atgctcgcgc cgggcacggt
     9301 cttcgcgcgg cgctatcgca tccaacgctt ccttgggtcg ggcgcgatgg gctacgtctg
     9361 ctgcgcgatc gatgagtcga tcgacgaggt cgtggcgctg aaggtgctct cgctgccgat
     9421 ccacgaggac cccgacgcgt acgatcgatt caagaccgag ttgaagctgg cgcgccgcat
     9481 ccgccatcgc aacgtcgtgc agagtttcga cctcgggttc gc
//
LOCUS       DATLVW010000939         4739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_289306, whole genome shotgun sequence.
ACCESSION   DATLVW010000939 DATLVW010000000
VERSION     DATLVW010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289306"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..2247
                     /locus_tag="VNN25_27810"
     CDS             97..2247
                     /locus_tag="VNN25_27810"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXH95414.1"
                     /translation="MTTQPWPLPPAESSPAESSSPGIATGIDRRLFCKLLGGGIVVLM
                     TSKPADLFAQRRGYPEDLNAYLRIDENGGVTLFSGKIEMGQGVHTSLAQMAAEELGVS
                     LDSITMVMGDTDLCPWDAGTWGSQSTRMFGPAVRAAAAEARAVLMKLASEKLGSPKEQ
                     LVVENGVVSNGSQKVTYGELAKGKEIARLVGEKAVLRTVKQFKAIGTPTKRMDAHEKV
                     TGRAKYAGDIRVPGMLYARILRPPMHGATRKSLDTSKAKAMSGVTVVEHDDLVAALHA
                     DPERAAEALALIKAEWDRPAAAFDTESVADYFVKSGGDGETGTSKGDVTTAAAGTHVF
                     QSTFRTGYVAHAPMEPHTALAEWKDGKMTVWASTQSPFGARSRIANVLGLDEKSVRVI
                     TPFVGGGFGGKSPTEQGVEAAQLAKITGKPVAVVWTRAEEFFFDTFGPASVIKINSSI
                     DDAGKMTMWDYNVYAAGGSSEVLYDVPNVRVKTYLGRRSGGEKVHPFATGPWRAPGAG
                     PNVFARESQIDIMAAAAKIDPLEFRLRNASDERLRRVLKAAADAFGWKAGAGPSGQGR
                     AIAVALDVGTYCAIAAEVAVDRKTGAINVKRIVAAQDMGLVINPWGAKMQMSGCLNMG
                     LGYVLSEELKFNGGDIVDKNFDTYELPRFAAVAPHIETILIKNDDLAPQGGGEPAIVP
                     MGAVIANAVFDATGVRMYRLPMTPERVLAAIKQA"
     gene            2752..2913
                     /locus_tag="VNN25_27815"
     CDS             2752..2913
                     /locus_tag="VNN25_27815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95415.1"
                     /translation="MLRVTRCDEREDKKRNNGTEALQSAFGRADDILVIGAGETIRVR
                     RRTGRESAT"
     gene            3135..>4739
                     /locus_tag="VNN25_27820"
     CDS             3135..>4739
                     /locus_tag="VNN25_27820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95416.1"
                     /translation="MKRIVIGAVIIIGLIGTANAGSNHAQSGAKPDLPDCRGIASPEQ
                     IASAKSAKSDPAFAFAKKHAKTAMPASVVCQSKLWQALHGNLPQPLSVEKDPALSASR
                     DNRERKQATSTGSVTPLAARPTPGPVGPPSEMKLQKASGKVFDLRTLPSTPPRKRERP
                     EREAPPVTPQPMDGGVPTVGESNSTVQAMAGPRSLANAPAPGPLTTFDGLDNANWGAG
                     HPPDTNGDAGPTYYIQTINTSIGIYRKSDGFRMAAFTFDTFMSQGNFGNLCDTDNFGD
                     PVVLYDSFEDRWIISDFAFQLDGSNNVINPPGSFQCFAVSMTGDPVSGGWNYYSINTT
                     GGLGDYPKLGIWPDGLYMSANMFDYAAAGAFQNSRLYAFNKAQMYAGAANIQSVSFNL
                     PSDQFTVLPANARLQTGTPPVGSPNYFASVWNFLNSVQIWKFHVDWGHPSLSSVTGPF
                     SSTMTFWWEQFARTTGVTTAPTPANFLDTLYPRLMVQNQYSNIGGVESLWNSHTVGAG
                     NPTTSVTSTQSAVRYYQLNVTGGTVAA"
BASE COUNT          937 a         1474 c         1493 g          835 t
ORIGIN      
        1 catcgtcgat gagatgaacg ggaacctctg ccgctgcggc gcgcatcagg gaatcatcgc
       61 cgcgatcgaa gacgcgtcga aggcaggagg tgcccgatga cgacgcagcc ctggcccctc
      121 cctccggcag aatcttctcc ggccgaatcg tcttcgccag gaatcgctac cggcatcgac
      181 cgccgccttt tctgcaagct cctcggcggc ggcatcgtcg tgttgatgac gtcgaagccg
      241 gccgatttgt tcgcgcagcg gcgcggctat cccgaggatc tcaacgccta tctgcggatc
      301 gatgagaacg gaggcgtgac cctcttctcc ggaaaaatcg agatgggtca gggcgtacac
      361 acgtcgctgg cgcaaatggc ggccgaggag ctcggcgtct cgctcgattc gatcaccatg
      421 gtcatgggcg acaccgatct ctgtccgtgg gatgccggca cgtggggatc gcagtcgacg
      481 cggatgttcg gcccagcggt gcgggcagcg gcagccgagg cgcgagcggt gctgatgaag
      541 ctggcgtcag agaagctcgg gagcccgaaa gagcagctcg tcgtcgagaa cggtgtggtg
      601 tcgaacggct cgcagaaggt cacgtacggc gagctggcga agggaaaaga gatcgcgcgg
      661 ctcgtcggcg agaaggccgt gctgcggacc gtcaagcaat tcaaggccat cggaacgccg
      721 acgaagcgga tggacgctca cgagaaggtg accggccgcg cgaagtacgc aggcgacatc
      781 cgcgtaccag gcatgctcta cgcgcgcatt ctccgcccgc cgatgcacgg ggccacgcgc
      841 aagtcgctcg acacctcgaa agcgaaggcc atgagtggcg tgacggtcgt cgagcacgac
      901 gaccttgtcg ccgcactgca tgcggatccc gagcgcgccg ccgaggcgct ggcgctgatc
      961 aaggccgagt gggatcggcc agccgccgcg ttcgacaccg agtcggtcgc cgactacttc
     1021 gtgaagagcg gcggtgacgg cgagaccggc acctcgaagg gcgacgtcac caccgccgcc
     1081 gcaggcaccc acgtcttcca gtcgacattc cgcaccggct acgtcgcgca tgccccgatg
     1141 gagccgcaca ccgcgctcgc cgagtggaag gacgggaaga tgacggtgtg ggcgtcgacg
     1201 cagagcccgt tcggcgcgcg ctcacgtatc gcgaacgtcc tcggcctcga cgagaagagc
     1261 gtgcgcgtca tcacgccgtt tgtcggcggc ggtttcggcg ggaagagccc caccgagcaa
     1321 ggcgtcgagg ctgctcaact ggcgaagatc accggcaagc cggtcgcggt ggtgtggacg
     1381 cgcgccgagg agtttttctt cgacacgttc ggccccgcct cagtgatcaa aatcaactcg
     1441 tccattgacg acgccggcaa gatgacgatg tgggactaca acgtctacgc ggctggcggc
     1501 tcgtcggagg ttctctacga cgtaccgaac gtccgcgtga aaacatacct cggccgtcgt
     1561 tccggtggcg agaaggtcca tccgttcgct accgggccgt ggcgcgctcc gggcgctggg
     1621 ccgaatgtct tcgcccgcga gtcgcagatc gacatcatgg ccgccgcggc gaagatcgat
     1681 ccgctcgaat tccgcctccg caatgcctcg gatgaacggc tgcgccgcgt tctgaaggcg
     1741 gcagccgatg cgttcggatg gaaagcgggc gcggggccat cgggtcaggg acgcgccatt
     1801 gccgtcgctc tcgacgtcgg cacgtactgc gcgatcgccg ccgaagtggc ggtcgatcgg
     1861 aagacgggcg cgatcaacgt gaagcgcatc gtcgccgctc aggacatggg gctggtcatc
     1921 aacccatggg gcgcgaagat gcagatgtcc ggctgcctca acatgggcct cggctacgtg
     1981 ctgagcgagg agttgaagtt caacggcggc gacatcgtcg acaagaactt cgacacctac
     2041 gagctgccgc gtttcgccgc agtcgcgccg cacatcgaaa cgatactgat caagaatgac
     2101 gacctcgcgc cacaaggcgg cggcgagccg gccatcgttc ctatgggcgc cgtgatcgcc
     2161 aatgcagtat tcgacgcaac cggcgtacgg atgtatcgcc tgccgatgac gccggagcgg
     2221 gttttggcgg cgatcaagca agcgtgagta cctgctcggg ggcacggaac aatcgcagag
     2281 agtggagaaa ctcacacacg atcggtagga gcttgcagcg cgctgccaag gtctcttggc
     2341 gtccaggagg tttctgtagt gcggacgcgg cggctaccga tctcgtgacc gtttctcacc
     2401 tcggccacgt caaacaagcc cgcatttctc gcagtctgga gttgtgcaca ctacgttttc
     2461 gatcgagcgc cttctccccc acgctttttt gtgggggaga aggtggcgaa gccggatgag
     2521 ggggtgtttc ctggcgcggg gaggcttgca tgacgggaga aaccagacct gtcgatcagc
     2581 ctttcgcgag agaacatcgc ttgtcgagag ttcgcccccc tcatccgcct tcggcacctt
     2641 ctccccccga agaagccggg gggagaaggc gctcgatgga agggagcgcc gaatttttca
     2701 atggtatgag aactgcgggc tagatgtgtc gcgccgcagc gatgtgcggt gatgttgcgc
     2761 gtgactcggt gtgacgagcg agaagacaag aagcgaaaca acggaaccga ggcgctgcaa
     2821 tcggcgttcg ggcgtgcgga cgacatcctc gtcatcggcg ccggcgagac gatccgagtg
     2881 cgacgacgga caggaagaga atccgccacc tgacctcgcg cacggggatg cgagttcagt
     2941 tcccctcaaa catcacgttg acaaccgtcc gcgtagcgac cattgttgcc gaaatatctt
     3001 tctggtgacg accggagaca accctcgtgt gccacctgcg gggttttgac gccggaacga
     3061 cgtagaacaa ttgcccgcag aacgctccat ctgtgtcgga tcggactaaa tccaacgctt
     3121 ctcgaggatc gcccatgaaa cgaatcgtca tcggagcagt catcatcatc ggtctgatcg
     3181 ggaccgcaaa cgcgggttcg aatcatgcgc aaagcggcgc gaaacctgat ctacctgact
     3241 gccgtggaat cgcttcaccg gaacagatcg caagcgcaaa atccgcgaag agcgatccgg
     3301 cctttgcgtt cgcgaagaaa catgcgaaga ccgcgatgcc cgcgtcggtc gtttgtcaga
     3361 gcaagctttg gcaggcgctg cacggaaacc tgccccagcc gctatccgtc gagaaagatc
     3421 ctgcgctttc ggcctcccgc gataaccgcg agcggaagca ggcgacttcg accggcagcg
     3481 taacgccttt agcggcgaga cccacgcccg gcccggtcgg tcctccatcc gagatgaagt
     3541 tgcagaaggc ctcgggcaag gtattcgacc tgcgcacgct gccctcaacg ccgccgagga
     3601 agagagagcg gccggagcgg gaagcgcctc ctgtaactcc gcagccgatg gatggcggcg
     3661 tgccgactgt cggtgaatcg aacagcacgg tgcaggcaat ggccggcccg cgctccctgg
     3721 ccaatgcccc ggcccccgga cctttgacga cgtttgatgg tctcgacaat gccaattggg
     3781 gtgcaggaca tccgccggac acaaacggtg atgccggtcc cacttactac atccagacaa
     3841 tcaacacctc gatcggtatt taccgcaagt ccgacggctt ccggatggcc gcatttacgt
     3901 tcgacacgtt catgagccag ggaaacttcg gcaatctctg cgacaccgac aacttcggtg
     3961 atccggtcgt cctgtacgac tcgttcgagg atcgctggat catttcggat ttcgcgttcc
     4021 agctcgacgg ctcgaacaac gtcatcaatc ctcccggctc gttccagtgt ttcgccgttt
     4081 cgatgaccgg cgatccggtc agtggcggct ggaactacta ctccatcaac accaccggcg
     4141 gtctcggcga ttatccgaag ctcggcatct ggccggacgg tctctacatg tcggccaaca
     4201 tgtttgacta cgccgcggcc ggagcgtttc agaactctcg tctctatgcc ttcaacaagg
     4261 cacagatgta tgcgggcgcg gcgaacatcc agtcagtatc gttcaacctt ccatccgacc
     4321 agttcacggt gcttcctgcc aatgcgcggc tgcagaccgg taccccgccg gtcggctctc
     4381 ccaactactt cgcatcggtc tggaacttcc tcaactcggt tcagatctgg aagttccacg
     4441 tcgactgggg tcatccctcg ctatcgagtg tcacgggacc gttcagttcg acgatgacgt
     4501 tctggtggga gcagtttgca cggacgacag gcgttacgac agcgccgact ccggcaaact
     4561 tcctcgatac actttatccg cgcctgatgg tgcagaacca atattcgaat attgggggcg
     4621 ttgagtcgct ttggaacagt cacaccgtcg gcgccggcaa tccgacgacg agcgtgacat
     4681 ccacacaatc cgcggtccgc tactaccagc tcaatgtgac cggtggaacc gtcgccgcc
//
LOCUS       DATLVW010000940         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_493558, whole genome shotgun sequence.
ACCESSION   DATLVW010000940 DATLVW010000000
VERSION     DATLVW010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493558"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VNN25_27825"
     CDS             <1..899
                     /locus_tag="VNN25_27825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95417.1"
                     /translation="DSRDKKRDAQLREDILAGRDEWSLRFANASPEADRVTFERARGE
                     LIRAEHVARIDVADLQKIFFDVAVSAVVTRGDGWAELAIYPGNSTRATRQQRDDSEKK
                     LRAYSRRAVRYFEAVRAMYDYMNDNPPRARVIFSTLFRDEDDPRPPLIDDKEHDLVEA
                     VRRAIDGLTKDEDTDALEAEAEMVFNPLPARIVVRVPSEPLLVEGFTRSKEGELIAEP
                     LSLLEAIASLEGRWVTPDPLAFSIRPGGGGDEEKEAALIAAMPRQTSAVIGEIEVADA
                     LVAKMRPAPRYRVRWITKARTD"
     gene            complement(903..1463)
                     /gene="rimI"
                     /locus_tag="VNN25_27830"
     CDS             complement(903..1463)
                     /gene="rimI"
                     /locus_tag="VNN25_27830"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXH95418.1"
                     /translation="MRAPRPSDRVARRRRALSRRHADLRPAGRGRSETATEAMTTEGI
                     IIERAAMGDIAAVHALEQRCFPATWRREFFESEVITPGRFNLVARRDGVIIAYLFAAW
                     IVDEMHINKIAVDAGARRQGIALALMERCTAFAVEQGVTSIWLEVRQSNEEAQQFYHL
                     LHFEPEYIRKGYYPDGEAAVVMTRDL"
     gene            complement(1346..1942)
                     /gene="tsaB"
                     /locus_tag="VNN25_27835"
     CDS             complement(1346..1942)
                     /gene="tsaB"
                     /locus_tag="VNN25_27835"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXH95419.1"
                     /translation="MLILAADTSLSILSAALLLDDALIGAVALEGKHSRNEKLLPAID
                     WLLTEASIERSAIDLFAVTRGPGSFTGVRIGLATMQGLALALGKPVCAMSTHEVIAPE
                     RGRAAIVDDAGRGEFYVSVFEDGQEVVAPHLAGRDEVESLEDVTRVPDFVQRSNVALA
                     CARRAQAIALRGDGERYRDVTPIYVRLAEAEVKLQQKR"
     gene            complement(2052..2507)
                     /locus_tag="VNN25_27840"
     CDS             complement(2052..2507)
                     /locus_tag="VNN25_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HXH95420.1"
                     /translation="MRAGWNEYFMGIAHQAATRSTCPRKRVGAVIVRDRTVLSTGYNG
                     SLRGLPHCDDAGCVMEDGHCVTTVHAEANAILQAAKNGIAIDGAELYTTASPCWNCFK
                     LIANAGIRKIYFGEFYRDERSREVAKETGIELIDLGAEPGSRESEVGAS"
     gene            2551..2913
                     /locus_tag="VNN25_27845"
     CDS             2551..2913
                     /locus_tag="VNN25_27845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXH95421.1"
                     /translation="MPSKAKVLIVEDNSDVRRLYAIGLNQRGFEVKLAANGAEAVERI
                     MVEKPDILLLDWLMPLMDGDEVLRRIGVPNGNGMPVIVISGQPEPDQRDPRIRSWLTK
                     PVSIDELVAEIERPVVAM"
     gene            2952..3428
                     /locus_tag="VNN25_27850"
     CDS             2952..3428
                     /locus_tag="VNN25_27850"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXH95422.1"
                     /translation="MAKLFFVGLDGTEKSYRLQTHRPFTVGRDPGNDIILRDPKVSRH
                     HAEIIFERGFFVVHDLSSANGTYINSKRVRVAPLTHAAKVRMGNTYGRFSEELPTESD
                     GMPAAEPFEQPLPIPTGSQGPFAAIESMQTSQLDRYSLSTQPHELLSEEDLKKKAE"
     gene            3578..4243
                     /locus_tag="VNN25_27855"
     CDS             3578..4243
                     /locus_tag="VNN25_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95423.1"
                     /translation="MSGIRAGETAYIRGEDGDALLSYRSFASVVGIIAALMSAVVLVA
                     GAAGVLFLLLERRPLPAIAAMILSAAFAAMIVMLIPATNATIFEGATPVITIAQQPGV
                     SFPTTTWIVSAPDGKPIVRIHKSFLARFGRNRWKMAGETSRGEAVEESLTRAIIRKLF
                     GKFARAYQSNLRLIWDGDEAGWIIRRTDALGRADVLDLHPALTLDRRAAVALAVLVLG
                     SEP"
     gene            complement(4247..>5150)
                     /locus_tag="VNN25_27860"
     CDS             complement(4247..>5150)
                     /locus_tag="VNN25_27860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95424.1"
                     /translation="DVLTYLAAALGIDRYAGVVTMPEDRSYYFPQTAAKETPPYRAAA
                     FLIKFGFLPAQAIDRADMNAAMPRDELYGLLGSWLRKHAAVSEATGKIVSVSARNVTM
                     KIDGKQTHFLLPANVLVFRKFGDRYQEYRNAPVTIGDRATIDSDVRKMPVALIINAYL
                     DGASFDRSSSFASWTRSFRADDLVVSINKRNPITKLQGIRPLTVDASERIAELEVTAE
                     NGRTFVLRGLPIRWSLNVPDNLFVYEKTQDPDGMDRYTFYGKGWGHGVGFCQVGAYGM
                     AMKGWTAQQILTHYYTGIEIQPMK"
BASE COUNT          984 a         1701 c         1567 g          898 t
ORIGIN      
        1 aggactcccg agacaaaaag cgcgacgcgc agttgcgtga agacatcctg gccggacgcg
       61 acgagtggag tctgcgcttc gccaacgcca gcccggaggc ggaccgcgtc accttcgagc
      121 gcgcacgcgg tgagctgatc cgcgccgagc acgtggcacg catcgacgtg gccgacctgc
      181 agaagatctt tttcgacgta gccgtctccg cggtcgtcac gcgcggcgac ggctgggcgg
      241 aactggccat ctatcccggc aactccacgc gcgcaacgcg gcagcagcgc gacgactcgg
      301 agaaaaagtt gcgggcctac tcccggcgcg ccgtccgtta cttcgaagcg gttcgcgcga
      361 tgtacgacta catgaacgac aacccgccac gcgcacgagt gatcttcagc acgctctttc
      421 gcgatgaaga cgacccacga ccgccgctca tcgacgacaa agaacacgat ctcgtcgagg
      481 ccgtcagaag agcgatcgac ggtctgacga aagatgagga caccgacgcg ctcgaagctg
      541 aagccgagat ggtcttcaac ccgctgccgg cgcgcattgt cgtgcgcgtc cccagcgaac
      601 cgctcctcgt cgaaggattc acgcgcagta aagaaggtga gctgatcgcc gagccgctgt
      661 cgctgctcga agcgatcgca tcgctggaag ggcgttgggt gactcccgat ccgctggcgt
      721 tctctatccg cccgggcgga ggcggcgatg aggagaagga agccgcgctc atcgccgcaa
      781 tgccgcgtca aacctcagcc gtcatcggcg agatcgaagt ggccgacgcg ctcgtcgcga
      841 aaatgcggcc ggcgccgagg tatcgggtac ggtggatcac gaaggcgcga acggactgag
      901 aatcacaggt ctctcgtcat caccacagcc gcctcgccgt ccgggtaata gcccttgcgg
      961 atgtactccg gctcgaaatg gagcaggtgg tagaactgct gcgcctcctc attcgactga
     1021 cggacctcga gccagatcga cgtcacgccc tgttcgacgg cgaaggctgt acagcgctcc
     1081 atcaatgcca gagcgatccc ctggcgccgc gctccggcat cgacagcgat cttgttgatg
     1141 tgcatctcgt cgacgatcca tgccgcgaaa aggtaggcga tgatcacgcc atcgcggcgg
     1201 gccacgagat tgaagcggcc cggcgtgatg acctcactct cgaagaactc gcggcgccac
     1261 gtagccggga agcagcgttg ctccagcgca tgcacggcag caatgtcgcc cattgcggcg
     1321 cgctcgatga tgatcccctc ggtggtcatc gcttctgttg cagtttcact tcggcctcgg
     1381 ccagccggac gtagatcggc gtgacgtcgc gatagcgctc gccgtcgccg cgcaacgcga
     1441 tcgcttgggc gcggcgcgcg caagcgaggg caacgttgct acgctggacg aaatcgggaa
     1501 cgcgcgtgac atcttcgagt gattcgactt cgtcgcgccc cgccagatgc ggcgcgacaa
     1561 cctcctgccc atcctcgaac accgaaacgt aaaactcgcc ccgccccgca tcgtcgacga
     1621 tcgccgcacg tccccgctcg ggagcgatca cctcgtgcgt cgacatcgcg cagaccggct
     1681 tgccgagtgc gagcgcgaga ccttgcatcg tggccaggcc gatgcgaacg ccggtgaacg
     1741 agcccggccc ccgagtgacc gcgaacagat cgatcgccga acgctcgatc gacgcctcgg
     1801 tcagcagcca atcgatggcc ggaagcagct tctcgttccg cgagtgcttt ccctccaacg
     1861 caaccgcgcc gatcagcgcg tcatcgagca gcagcgcggc agagagaatc gagagcgacg
     1921 tatccgcggc gaggatcagc atgtgtgcgg gggtcgcgtg tcgggtgtcg gggatcggag
     1981 agattttggg caagtagcag acgcaaaatt ggggaacgaa ccgttgggag ccgatgcgga
     2041 gtggatcgga atcacgacgc tccgacctcc gactcccgac tccccggttc cgcccccaga
     2101 tcgatcaact cgatccccgt ctcctttgcc acttcccgcg agcgctcgtc ccggtaaaac
     2161 tcaccgaagt aaattttgcg gattccggcg ttggcgatga gcttgaagca gttccagcag
     2221 ggcgatgccg tggtgtacag ctcggcgccg tcgatcgcga tgccattctt ggccgcctgc
     2281 aggatcgcat tggcctcagc atgcacagtc gtgacgcagt gcccatcctc catcacgcag
     2341 ccggcgtcgt cacagtgcgg caaacctcgc agcgagccgt tgtaaccggt cgaaagcact
     2401 gtacgatcac gcacgattac cgccccgacc cgtttgcgcg gacaggtcga gcgcgtggct
     2461 gcctggtgcg cgattcccat gaagtattcg ttccatccgg cacgcatgac cgggattcta
     2521 catgcgcacg aaacctgcag agctgccaac atgccctcaa aagccaaggt gctcatcgta
     2581 gaggacaact ctgacgtccg gagactctat gcaatcggcc tcaaccagcg tggcttcgag
     2641 gtaaagcttg ccgcgaatgg tgccgaagcg gtcgaacgga tcatggtcga gaagccggac
     2701 atcctgctcc tcgactggct catgcccctc atggacggtg acgaagtgct gcgccggatc
     2761 ggcgtaccca acggtaacgg gatgcctgtc atcgtgatca gcgggcagcc agagcccgac
     2821 cagcgcgatc cacgcatccg aagctggctc acgaaaccgg tctcgatcga cgagcttgtc
     2881 gccgagatcg agcggccggt cgtggccatg taggggcacc ttgcggcaat cggctacaat
     2941 tcgttccatc catggccaag ctctttttcg tcgggctcga cggcactgag aagagttatc
     3001 gcttgcagac gcaccggccg ttcacggtcg gacgcgatcc cggcaacgac atcatcctcc
     3061 gcgacccgaa agtctcacgg catcacgccg agatcatctt cgagcgcgga ttcttcgtcg
     3121 tccacgacct ttccagcgcc aacggcacct acatcaacag caaacgcgtc cgcgtcgccc
     3181 ctctgaccca cgccgcgaag gttcgcatgg gcaatacgta cggacgcttc tcggaagagc
     3241 tgcctaccga gagcgacggg atgcctgccg cggagccgtt cgaacagccg ctgcccattc
     3301 ctaccggcag ccagggcccc ttcgccgcga tcgagtcgat gcagacatca caactcgacc
     3361 gttacagcct ctccacccaa ccgcatgagc tactctccga ggaagatcta aaaaaaaaag
     3421 cggagtagag gatgacgatg cgacggtaac gacggccgat ccgccgaccg agcctcttct
     3481 tgatcttccg ccgcctccgc cagccaaacc cgcaccgccc actccccctg ccacgccacc
     3541 gcccgcggct gtgttcactc gttcgcgcta ccagttcatg agcggcatcc gcgcgggcga
     3601 gacggcgtac atccgcggag aagacggaga tgcccttctg tcgtaccgaa gctttgccag
     3661 cgtcgtcggc atcatcgccg cgctgatgtc ggcagtcgtg ctcgtcgccg gagccgccgg
     3721 cgtgctgttc ctgttgctgg aacggcggcc gctgccagcc atcgccgcca tgatcctcag
     3781 cgccgccttt gccgcaatga tcgtcatgct cattccggcg acgaacgcaa cgatcttcga
     3841 gggagcgact ccggtgatca cgatcgcaca gcagccgggc gtcagcttcc cgacgacaac
     3901 ctggatcgtc tccgctccgg atggcaagcc gatcgtgcgc attcataaat cgtttctcgc
     3961 ccgtttcggc cgcaaccgct ggaagatggc cggcgagacc tcgcgcggcg aagcggtcga
     4021 ggagtcgctc acccgcgcca tcatccgcaa actcttcggc aagttcgccc gcgcttacca
     4081 gtcgaacctc cgcctgattt gggatggcga tgaagccgga tggatcatcc gccggaccga
     4141 tgcgctcggc cgcgccgacg tgctcgatct gcatcccgcg ctgacactcg atcgccgcgc
     4201 ggcggtggcg ctggcggtgc tggtgttggg ctcggagccg tgacccctac ttcatcggct
     4261 gaatctcgat gccggtgtag tagtgcgtca ggatctgctg ggcggtccat cccttcatcg
     4321 ccattccata cgcgccgacc tggcagaagc cgacgccgtg gccccatccc ttgccgtaga
     4381 acgtgtagcg gtccatgccg tcgggatcct gggtcttctc gtagacgaac aggttgtcgg
     4441 gcacgttgag cgaccagcga atcggcagac cgcgcaacac gaacgtgcgc ccgttttccg
     4501 ccgtcacttc gagctcggcg atgcgctccg atgcgtcgac cgtcagcggg cggataccct
     4561 gcagtttcgt gatcggattg cgcttgttga tcgagacgac gagatcgtcc gcgcggaaac
     4621 tgcgcgtcca actggcgaag ctcgagcttc ggtcgaatga cgctccatcg agataggcgt
     4681 tgatgatcag cgccaccggc atcttgcgaa cgtcggaatc gatcgtggca cggtccccga
     4741 tcgttaccgg cgcgtttcgg tactcctgat agcgatcgcc gaacttgcga aagacgagaa
     4801 cgttggccgg caagagaaag tgcgtctgtt tgccgtcgat cttcatcgtc acgttgcgcg
     4861 ccgacacgct gacgatcttg ccggttgctt cgctgactgc cgcatgtttg cgaagccacg
     4921 agccgagcag gccatatagc tcatcgcgcg gcatcgccgc gttcatatct gcacggtcga
     4981 tcgcctgcgc aggtaggaag ccgaacttga tgagaaacgc cgcggcacga tacggcggcg
     5041 tctctttggc cgcggtctgc gggaagtagt agctgcggtc ctcaggcatc gtcacgacgc
     5101 cggcgtagcg atcgatgccg agcgcagcgg cgagatacgt gaggacatcg
//
LOCUS       DATLVW010000941         6752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_617962, whole genome shotgun sequence.
ACCESSION   DATLVW010000941 DATLVW010000000
VERSION     DATLVW010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617962"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1700
                     /locus_tag="VNN25_27865"
     CDS             141..1700
                     /locus_tag="VNN25_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXH95425.1"
                     /translation="MEVNPLATPNDVAGLDFERDIAFPGEFPYTRGIHPGGYRGRLWT
                     MRQFAGFGSATHTNERFKYLLSQGQTGLSTAFHLPTLYGYDSDHHFSAGEVGKCGVAI
                     DTLADMETLFDGINLEDVTVSMTINSTGPIALAMYLVVAEKQGADWKKISGTLQNDIL
                     KEYIAQKEYIFPPRPSMRLITDTFRFCAENIPKYNPISISGYHIREAGSTALQELAFT
                     LRDGMEYVEYGVKAGLDIDDFAPRLSFFFNSHNDLFEEVAKFRAARRVWAKVMRDRYG
                     AKDPRSWQLRFHTQTAGCSLTAQQPYNNIVRVAIQALAAVFGGTQSLHTNSLDETLAL
                     PTEQAARIALRTQQIIAHESGVINSVDPLGGSFFVEELTNRMEQGCFDYIARIDAYGG
                     MVEAIEAGFPQREIWDAAYQFQRSVDSGEKVVVGMNAFKMETEEPYDALYIDDSVAAE
                     QVERLNRVRADRDATKVVDAIQSLRNAAADPNVNTMPFIIDAVRVYASVGEISDALRD
                     VFGTYQEPAAF"
     gene            1826..6679
                     /locus_tag="VNN25_27870"
     CDS             1826..6679
                     /locus_tag="VNN25_27870"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HXH95426.1"
                     /translation="MTITPEQRKADLVERLATEAHKRVKEEEAESAEAFVRRYFALVA
                     PDDIIYTAFDTLLGGALSLWQFGSQREVGSPKIRLFNPTTETNGWGSEHTVIEVVNDD
                     MPFLVDSVSAEINRLGRNIHLLLHPVVRVRRDSAGRRVELTSTHDAPKDAMVESYMHV
                     EMDQETDAAELESMRAAIERVLHDVRAAVTDWQAMQKQLKADVAELDPAKLPMPEEEV
                     SEARAFLEWLDKGNFILLGYRRYGFETRNGKDYLPVVPGTGLGILRDVRAESAARGTA
                     PLTTEFSDYARAKDLLIITKANSRSTVHRPVPLDRIGIKRYNDKGDLIGEDRFLGLFT
                     SAAYSRSVREIPMLRLKAMRTIEQAGLDPHSHNGKALVDILETFPRDEFFQITDAQLY
                     EIARGILLLQERHRVALFTRKDVFERFISCYVYVPRDQNTAEFREKAKQILEENFGGR
                     DTAIYEHVTDAPLARILFVVRTTAGQIPEVDEKRIEAILSDAARTWADRLLDALCVNH
                     GEEQGIELQRRYKKAFSAAYMERFRAGEAMYDIQHVEEVLATGKLVVDLYRHRGGERQ
                     FHCKILHSGPPVPLSEIMPRLENMGLKVLAEEPYEVHPAGAASLVRIRDFNLDAEGMQ
                     DDLTPIKEKFQEAFVRVWSREAESDGFNRLVLAAELEWHEIVVLRAYARYMRQIGVNV
                     SEPLIQQTLATHAAVARLLIQLFQTHFDPSLGAATLRGASMASPGVTGRHMAAGGIRT
                     LVEDALDKVKNPDEDRILRLYLTLIEATLRTNYYQRDASGNRKAYLSFKLDSQSIREL
                     PLPRPMFEISVYSPTMEGIHLRAGKVARGGIRWSDRREDFRTEILGLMKAQNVKNVVI
                     VPMGSKGGFVVKNPSADRATFQKEGVAAYQTLLRGMLDITDNLRGTDVIPPRDVVRRD
                     ADDPYLVVAADKGTATFSDIANAISAEYGFWLGDAYASGGSAGYDHKAMGITARGGWE
                     AVKRHFRELGTNIQREDFTAVGVGDMSGDVFGNAMLLSPHTKLIAAFNHVHIFLDPNP
                     DPVTSLAERQRMFDERLGWNGYKPELLSKGGAVLERSAKVVTVSEEVMRALDLERKTL
                     APAELMKAILRARADLLWLGGIGTYVKSSDEDHAAARDRANDALRVDASELRVRVVGE
                     GANLGFTQSGRIEYALREGHINTDAIDNSAGVDTSDHEVNIKILLYDAIEQGELAGIE
                     ERNKQLASMTDEVGQLVLCDNYEQTQAISLTQALGESVLDEQARFMRALERAGRLDRA
                     LEGLPDDETIAQRHTAHIGLTRPELSVLMAYSKIGLFSDLLASDLPDDPLLVEDLVLY
                     FPKPLRQQYRAAIERHRLRREIIGTYVTNNIINRIRPTFVWQMTEETGKPTADVARAF
                     TIIRESFDLRSLWSEIEAQDNILPARVQIEMLTEVGRLLERAIGWLLISDYDRLDTAK
                     YVAEFRPAIAAIADRLHDILPAALLGSLKARQGELEMDGVPERVAYRIASLGVMGAAL
                     DIVRLSRTGRPVQDVAKVYFSVGARFGLERLRSAGASIAAETPWQKAAVAAAVDDLFN
                     YQSVIASRVLGESNGAGEPLDGWLTARPRVVERIDQMMTDFRAAQTVDLAMLTVASRQ
                     LRALVES"
BASE COUNT         1307 a         2302 c         2007 g         1136 t
ORIGIN      
        1 caaacgaaca gctcccgctc ttctccgcag aggcgttgag cgcgattcgc aaagaagaag
       61 caagctggcg ccgcgacacc ttcgcgccgc tcacggcgaa gaaagctccc tggaagaaag
      121 acttcaccac cgtcagcgcg atggaggtca atccgctggc cacgcccaac gatgtcgctg
      181 gcctcgactt cgagcgcgac atcgccttcc ccggcgagtt tccctacaca cgcggcatcc
      241 acccgggcgg ataccgcggc aggctctgga cgatgcggca gttcgccggt ttcggcagcg
      301 ccacgcatac caacgagcga ttcaagtacc tgctctcgca gggacagacc ggcctctcca
      361 cggcgttcca tctgccgacg ctttacggct acgactccga tcatcacttc agcgctggcg
      421 aggtcggcaa gtgcggcgta gccatcgaca cgctggccga catggagacc ctcttcgacg
      481 gcatcaacct cgaggacgtc accgtctcga tgacgatcaa ctccaccggc cccatcgccc
      541 tggccatgta tctggtcgtt gccgaaaagc agggagcgga ctggaagaag atctccggca
      601 cgctgcagaa cgacatcctc aaggagtaca tcgcccagaa ggagtacatc ttcccgccgc
      661 ggccttccat gcggctgatc accgatacgt tccgcttctg cgccgagaac attccgaagt
      721 acaacccgat ctcgatcagc ggctatcaca tccgcgaagc cggctcgacc gcgcttcagg
      781 agctggcatt cacgctccgc gacgggatgg agtacgtcga gtacggcgtc aaagccggac
      841 tcgatatcga cgatttcgcg ccgcgtctct ccttcttctt caactcgcac aacgacctgt
      901 tcgaggaagt tgcgaagttc cgcgcggcgc gccgcgtgtg ggccaaagtc atgcgcgacc
      961 gctacggcgc gaaggacccg cgctcgtggc agcttcgttt ccacacccag accgcaggct
     1021 gttcgctgac cgcgcagcag ccgtacaaca acatcgtccg cgtcgccatc caggcgctgg
     1081 cggcggtctt cggcggtacg cagtcgctgc acacgaactc gctcgatgag acgctggcgc
     1141 tgccgacgga acaggcagcg cgcattgcgc tgcgcacgca gcagatcatc gcgcacgaat
     1201 ccggcgtcat caactccgtc gatcccctcg gcggctcgtt cttcgtcgag gaattgacga
     1261 accggatgga gcagggctgc ttcgactaca tcgccaggat cgacgcttac ggcgggatgg
     1321 tcgaggcgat cgaggccggc ttcccgcagc gcgagatctg ggatgcggcc taccagttcc
     1381 agcgctcggt cgactccggc gagaaggtcg tcgtcggcat gaacgcgttc aagatggaga
     1441 cggaagagcc gtacgacgcc ctctacatcg acgattcggt cgctgccgag caggtcgagc
     1501 ggttgaatcg cgtgcgagcc gatcgcgatg caaccaaagt agtggacgcc atccagtcgc
     1561 tccgaaatgc cgctgccgat cccaacgtaa acacgatgcc gttcatcatc gacgccgtcc
     1621 gcgtttacgc cagcgtcggc gagatcagcg acgcccttcg cgatgtcttc ggcacctatc
     1681 aggagccggc ggcgttctag cggtcgaaag tctcgcgcgg gcggctgtgg ccgcggtcac
     1741 gtgagctcgt gaccgcgttc gtgacctcgg accccgcagg tcatatgatc tgtccccgca
     1801 tcgtcaccac gcaggagtct cgttcatgac catcacccca gagcagcgca aagccgatct
     1861 tgtcgagcgg cttgctacgg aagcgcacaa acgcgtcaaa gaggaagaag cggagagcgc
     1921 cgaagcattt gtgcgccgct actttgccct cgtcgcgcct gacgacatca tctacacggc
     1981 cttcgatacg cttctcggcg gggcgctctc cctctggcaa ttcggttcgc agcgcgaagt
     2041 gggatcgccc aagatacggc tgttcaatcc gactacggaa accaacggat gggggagtga
     2101 gcacacggtc atcgaagtcg taaatgacga catgcccttc ctcgtcgact cggtcagcgc
     2161 cgagatcaac cggctcggcc gcaacatcca tctgctgctt catcccgtcg tgcgcgttcg
     2221 ccgcgactcc gccggccgcc gcgtcgagct cacgtcgacg catgacgcgc ccaaagacgc
     2281 catggtcgag tcgtacatgc acgtggagat ggatcaggag accgacgccg ccgagctcga
     2341 gtcgatgcgc gccgcgatcg agcgggtgct tcacgatgtg cgcgccgccg ttaccgactg
     2401 gcaggcgatg caaaagcagc tcaaggcgga tgtcgccgag ctcgatcccg cgaaactgcc
     2461 gatgccggaa gaagaagtca gcgaggcgcg cgccttcctc gagtggctcg ataagggcaa
     2521 cttcatcctc ctcggctacc gccgctacgg attcgagacc cgcaacggga aggactatct
     2581 gcccgtcgtc cccggcaccg gcctcggcat ccttcgcgac gtccgcgccg agtctgcggc
     2641 gcgcggaact gcaccgctca ccaccgaatt cagcgactac gcgcgcgcga aagacctcct
     2701 catcatcacg aaggccaaca gccgcagcac cgtgcatcgc cccgtgccgc tcgatcgcat
     2761 cgggatcaaa cgttacaacg acaagggcga tctgatcggc gaggaccgct tcctcgggct
     2821 cttcacctct gccgcgtaca gccgcagcgt gcgcgagatt cctatgctgc gcctcaaggc
     2881 gatgcgcacg atcgagcagg ccggactcga tccgcacagt cacaacggca aggcgctcgt
     2941 cgacatcctc gagacctttc cgcgcgacga gttctttcag attaccgacg cgcagcttta
     3001 cgagattgcg cgcggcatcc tcttgctcca ggagcgccac cgcgtcgcgc tcttcacgcg
     3061 caaagacgtc ttcgagcgct tcatctcctg ctacgtctac gttccgcgcg accagaacac
     3121 ggccgagttc cgcgagaaag cgaagcagat cctcgaagag aatttcggcg gacgcgatac
     3181 cgcgatttac gagcacgtca ctgatgcgcc gctggcgcgg attcttttcg tcgtccgcac
     3241 cactgcgggg cagatccccg aggttgatga gaagcggatc gaggccatcc tctccgacgc
     3301 cgcgcgcacc tgggccgacc gtctgctcga cgcgctctgt gtcaatcacg gtgaggagca
     3361 gggcatcgag ctgcagcgcc gctacaagaa agccttctcc gcggcataca tggagcgctt
     3421 ccgggccggc gaggcgatgt acgacatcca gcacgtggaa gaagtgctgg ccaccggcaa
     3481 gctcgtcgtc gatctctacc gtcatcgcgg cggtgagcgc cagttccact gcaagatcct
     3541 gcactcaggt ccgccggtgc cgctatccga gatcatgccg cggctcgaga acatgggcct
     3601 caaggtcctc gccgaagagc cttacgaagt gcatcccgcg ggcgcagcgt cgcttgtgcg
     3661 gattcgcgac ttcaatctcg acgccgaggg gatgcaggac gacctcacgc cgatcaagga
     3721 gaagtttcag gaagcgttcg tgcgcgtctg gagccgcgaa gccgagagcg acggattcaa
     3781 tcgactcgtc ctcgccgccg agctcgaatg gcacgaaatc gtcgtcctgc gggcctacgc
     3841 gaggtacatg cggcagatcg gcgtcaacgt cagcgagccg ctgattcagc agacgctcgc
     3901 cacgcacgcc gccgtggccc gactcctcat tcagctcttc cagacacact tcgatccctc
     3961 gcttggcgcg gcaaccctgc gtggcgcgag catggcctcg cccggcgtga ccggacggca
     4021 catggccgcc ggcggaatcc gtacgctggt cgaagacgcg ctcgacaaag tgaagaaccc
     4081 cgatgaggac cgcatcctgc ggctttacct cacgctcatc gaggccacgt tgcgaacgaa
     4141 ttactaccag cgcgatgcga gtggcaatcg caaggcgtac ctctcgttca agctcgactc
     4201 gcagtcgatc cgcgagctgc cgctgccgcg tccgatgttc gagatctccg tctactcgcc
     4261 gacgatggaa ggcatccatc ttcgcgccgg caaggttgcg cgcggcggca tccgctggtc
     4321 ggaccgccgc gaagatttcc gcaccgagat cctcggcctc atgaaagcgc agaacgtgaa
     4381 gaacgtcgtc atcgtgccga tgggatcgaa aggcggtttc gtcgtcaaga atccttcagc
     4441 cgaccgcgcc acattccaaa aggagggcgt ggccgcgtac cagacgctcc tgcgcggcat
     4501 gctcgacatc accgacaacc tgcgcggcac cgacgtcatc ccgccgcgtg atgtcgtccg
     4561 ccgcgatgcc gacgatccgt atctcgtcgt cgccgcggac aagggcaccg cgacgttctc
     4621 cgacatcgcc aacgccatct ccgccgagta cggcttctgg ctcggcgacg cctacgcctc
     4681 gggcggcagc gccggctacg accacaaggc aatgggcatc accgcgcgcg gtggatggga
     4741 ggcggtcaag cgccacttcc gcgagttggg gaccaacatt cagcgcgagg acttcaccgc
     4801 ggtcggtgtc ggcgacatgt ccggcgacgt cttcggaaac gcaatgctcc tttcgccgca
     4861 caccaagctc attgcggcgt tcaatcacgt gcatatcttt ctcgacccga atcccgatcc
     4921 ggtcacgagt cttgccgagc gtcagcggat gtttgacgaa cgcctcgggt ggaacggcta
     4981 caagcccgaa ttgctgtcca aagggggcgc cgtactcgaa cgaagcgcga aggtcgtgac
     5041 cgtttccgaa gaagtgatgc gtgctctcga tctcgaaagg aagacgctcg ctccggccga
     5101 actgatgaag gccattctcc gcgctcgcgc cgacctgctc tggctcggcg gcatcggaac
     5161 ttacgtcaag tcgagcgacg aagatcatgc cgccgcgcgc gaccgcgcga acgacgcact
     5221 ccgcgtcgat gccagcgagc ttcgcgtacg cgtcgtcggc gagggcgcaa acctcggctt
     5281 cacgcagagc ggccgcatcg agtacgcgct gcgcgaaggt cacatcaaca ccgacgccat
     5341 cgacaactcc gccggcgtcg acacctcgga ccacgaggtc aacatcaaga tccttcttta
     5401 cgacgccatc gagcagggcg agctggccgg catcgaagag cgcaacaaac aactcgcgtc
     5461 gatgacggac gaagtcgggc aactcgtcct ctgcgacaac tacgagcaga cccaggccat
     5521 ctcgctgacg caggccctcg gcgagtctgt gctcgacgag caggcccgct tcatgcgcgc
     5581 actcgaacgc gccggccgcc tcgatcgcgc gctcgaaggg ctgcccgacg acgagaccat
     5641 cgcgcagcgg cacaccgccc acatcggcct cacgcgtccc gagctgtccg ttctcatggc
     5701 ctacagcaag atcggcctct tctccgatct gctcgccagc gatctgccgg atgatccgct
     5761 cctcgtcgaa gacctcgtgt tgtatttccc gaaaccgttg cgccagcaat accgcgccgc
     5821 catcgagcgc caccgtctgc gccgcgagat catcggcacc tacgtcacca acaacatcat
     5881 caaccgcatc cgcccgacgt tcgtttggca gatgaccgag gagacgggga agccgacggc
     5941 ggacgtggcg cgcgcgttca cgatcatccg cgaatcgttc gatctccgct cgctctggtc
     6001 cgagatcgaa gcgcaggata acatcctgcc ggcacgcgtg cagatcgaga tgctgacgga
     6061 agtaggacgc ctgctcgagc gcgccatcgg ctggcttctc atcagcgact acgaccggct
     6121 cgacaccgca aagtacgttg ccgaatttcg tcccgccatc gccgcaatcg ccgatcggtt
     6181 gcacgacatc cttcccgccg cgctcctcgg ctcactcaaa gcacggcagg gcgagctgga
     6241 gatggacggc gttcccgagc gcgtcgccta ccgcatcgcc agcctcggcg tgatgggagc
     6301 cgccctcgac atcgtccgcc tctcgcgcac cggtcgcccc gttcaggatg tcgccaaggt
     6361 ctacttcagc gtcggtgcac gtttcggact ggagcgcctc cgctccgccg gcgcgtcgat
     6421 tgccgcggaa acgccgtggc agaaagcagc agtggcagcg gcggtcgatg atctcttcaa
     6481 ctaccaaagc gtcatcgcgt cgagagtcct cggcgaatcg aacggcgccg gagagcccct
     6541 cgacggatgg ctgaccgccc gcccgcgcgt cgtcgagcgc atcgaccaga tgatgaccga
     6601 cttccgcgcc gcgcaaaccg tcgatctggc gatgctgacg gtcgcaagca ggcagttgcg
     6661 tgctcttgtg gagagctaga agaatcatca cagagacaca ggggacacag agctgaagcc
     6721 cgtcctctgt gtcctctgtg tctctgtggt ga
//
LOCUS       DATLVW010000942         6373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_576529, whole genome shotgun sequence.
ACCESSION   DATLVW010000942 DATLVW010000000
VERSION     DATLVW010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576529"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..1412
                     /locus_tag="VNN25_27875"
     CDS             561..1412
                     /locus_tag="VNN25_27875"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXH95427.1"
                     /translation="MKQIVVIGAGTMGNGIAHTAAASGFDVTLIDVAEAFLERGMSTI
                     SGNLQRGVDKGKITAEEKQQVLGRIRATSDIASASDAEIVIEAIIENLAAKAQLFEKL
                     DALTRPDCILASNTSSISITKIAAATRRPDKVIGMHFMNPVPVMTLVEVIRGIATSDE
                     TYGRVEQLSKQMGKTAIEVNDYPGFISNRVLMPMINEAIFALFEGVATAESIDGVMKL
                     GMNHPMGPLTLADFIGLDVCLAILRVLEEGFGDPKYRPCPLLVKMVDAGWLGRKSGRG
                     FYDYRKS"
     gene            1460..3316
                     /locus_tag="VNN25_27880"
     CDS             1460..3316
                     /locus_tag="VNN25_27880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXH95428.1"
                     /translation="MLCQTCSALNDDEREYCFRCQAKLLVLSGVNSFEEDEVEEYDEE
                     GDVSLDEHLLERVSGLEEIVKRSAETIRSIVDAIQKHERAIFINQTGLLSVKELLERK
                     NVVSADELVELWESKMGEQMLALEKKQRFTERKDRIMSLFRGERRERFVQLVAEAESA
                     IDAFDPDRGIKALEEAFKLDRDNYELSFFLGEMFFNDGNLDRAKHYLERTLEVQPDHF
                     DAAVFYGVLLHERQDLKGAERWLRRAIQISQESFLPYFSLGAIYARKGKLLRAQKFLE
                     KAIQIEQLPQAHYLLGTIFYDKGQLERAIKSFQSALKLDPEYEEAIYHLGLCYLDRNW
                     NRKAIDCFQEALELNPNKIEYQQAVKIYEGISGHIPLEGPAADEFKNAEVLATSGKYL
                     EALDHYRRASAVDPDNTSILMPYALLCSHLEMNTEAIAAARRVLRQRPTDVVAAAAYT
                     TLVEALRAEGNFKDANRALEEMLRDYTSNYAKSIAYYEKAYNLAEMEESLDEALESAQ
                     LALRYSPKELKQFPLAALGWVYFKRREFDNAVDFLTKSADLGPTATNLMHLGMALLES
                     GQKDRARQVFRKAKSFKAQGAGLEEKILEQIRSTTKLIDRLHAKRRRTANKA"
     gene            complement(3331..3750)
                     /locus_tag="VNN25_27885"
     CDS             complement(3331..3750)
                     /locus_tag="VNN25_27885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95429.1"
                     /translation="MAIAALHRGVIFRAEHDDSYPFWGDNEPPRSKLCLVFNADPVQE
                     GDVHYFLATSKVDRYRESPSILSDVLILPPASYPFFPRETLIDFRRLCVVSIEKLVTK
                     RLTLRGVLSDEDIRRCEDVAAKARILDNRHKRLLRLR"
     gene            complement(3760..4341)
                     /locus_tag="VNN25_27890"
     CDS             complement(3760..4341)
                     /locus_tag="VNN25_27890"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HXH95430.1"
                     /translation="MKTIRFRYDQDKVMNALAFFSQNGVADLDQMKTSKLLFLADRAH
                     LLRYGRTITGDTYVCMENGPVPSTTRDLMNARFADDPDDVSMREYFDVRHSSTYPQLI
                     ARHEPDLDVFSDSDIEVLRETVEVYGKKTAWQLRELTHEFPEIGAALAARSAAKRNVI
                     PIPFESFFDPSAPMIELVRQDQDDRDFAESLTW"
     gene            complement(4503..6062)
                     /locus_tag="VNN25_27895"
     CDS             complement(4503..6062)
                     /locus_tag="VNN25_27895"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXH95431.1"
                     /translation="MKPTIRILIAVAALFPATHLLATTFQPNNRIVRQLTIEPGGSVW
                     IDNPFGSIDVIGGEGTLISVTADRSIVASDAVALKEAADAVAIAFDGDSKVRLIRTVM
                     PALRNAHATIDYTIRVPKNVAVKIGSKSADHIRVSHITGSVTVNGFAGTVIMEDVSGA
                     SALNVVNGRVIYDYQQNPIAKADVQAVNADIDVYVPASAKFEWLADTINGDILTTFPI
                     HGGRFAGTIFRGQVNGPGGPTINTATMMGRVLLLAKGSNPGQASSMRLNPPQHRPTPG
                     DVMLAPAVKVQLPFHNGDWPFVASIADIAIGEIRGNANIRIGAGEVELGAVFGQCTVT
                     LMGGPLNLGDITGLLSAHTGAGDILVRAARMGGEVSTDGGLLRVLYTGGPMTLRSGGG
                     DIIVGQTSGAIDAQTTSGDITITGDPRHRTLKVGARTTQGNVNLIVSPRFGADIDATI
                     VTSDPDVNEMHIDFTGLTVRREQAGAKTRIHATGKINGGGERVELYAEEGDIHISAQT
                     ITPVRLANPRR"
     gene            complement(6059..>6373)
                     /locus_tag="VNN25_27900"
     CDS             complement(6059..>6373)
                     /locus_tag="VNN25_27900"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXH95432.1"
                     /translation="TEALANVLRNDEHQGVRIKAADTLQSMQSAMTDGTRDALVEALK
                     NDPNPAVRVKAVEALAKLARSGAVLDPDMLDTLRKKAAQGDENVYVRVKAAEALSSIQ
                     PR"
BASE COUNT         1291 a         1935 c         1919 g         1228 t
ORIGIN      
        1 tcttcggtga cgaacttcac gctgcggtcg gcagaaagga cgatcgccgg cgcgcccgcc
       61 gccatgagga cgttagcgat gctggtaatt gccggcgagg ccgactcgac aggaaacggc
      121 agaagaggcg ctttcgggaa aggttccagt tccggagcag gcgccatggc cgtagcggcc
      181 gcggcctggc cgccgcttcg cggagctgat gtctcgcgtg gcggttgcgg ctgaggctgc
      241 gctttcggtg gggcgggtgg cggcgcgctc gcgcgttgcg gctcaacggg agggggagag
      301 gtctgttccg agactgcgct agcgggcgtg ggtggcttcg attttctggg cttgtcgcaa
      361 cgctcgcaga ggattccccg cgtaatcgcc gcaccgcaca tgaaacattt cgaggccatg
      421 gaacaaactc aggtggggga tcttacgccc gctactcaca gagctgcaag atgatagctc
      481 acgggtcagt ttattacagc catcgcgatt caacctgacc cacagccaga tgataggctt
      541 cgccgccgtc ggagggcttc atgaaacaga tcgtcgtgat cggcgcaggc accatgggaa
      601 acggcattgc gcacactgcc gccgcatctg gttttgacgt aaccctcatc gacgtggccg
      661 aggcattcct cgagcgcggc atgtcgacga tctccggcaa cctccagcgc ggcgtcgaca
      721 aaggaaagat caccgccgag gaaaagcagc aggttctcgg gcggattcgc gccacgagcg
      781 acatcgcctc tgcctctgat gccgaaatcg tcatcgaggc gatcatcgag aacctcgctg
      841 ccaaagccca gctcttcgag aagctcgatg cgctcacgcg gcccgactgc attctcgcct
      901 cgaacacgtc gtcgatctcg atcacgaaga tcgccgctgc gaccaggcgg ccggacaaag
      961 tcatcggcat gcacttcatg aatcccgtgc cggtcatgac gctcgtcgaa gtcatccgcg
     1021 gcatcgccac cagcgacgag acctatggcc gcgtcgaaca actctccaaa cagatgggca
     1081 aaacggcgat cgaggtgaac gactatcccg gcttcatctc caatcgcgtt ctgatgccga
     1141 tgatcaacga ggcgatcttc gccctgttcg aaggcgtggc aacagccgaa tcgatcgacg
     1201 gcgtgatgaa gcttggcatg aaccatccga tggggccact gacactggcc gacttcatcg
     1261 gactcgatgt ctgtctggcc attcttcgcg tgctcgaaga aggattcggc gatccgaaat
     1321 accgtccctg tccgctgctg gtcaagatgg tcgatgccgg ctggcttggc cgcaagagcg
     1381 gccgcggttt ttacgactat cggaagtcct gaataacagg gatgacgtaa cgcctcagcg
     1441 gtgttctaat cgccaccaca tgctctgcca gacctgtagc gcgttgaacg atgacgagcg
     1501 ggagtattgc tttcgttgtc aggcgaaact tctcgttctc tccggagtga actcgttcga
     1561 agaggacgaa gtcgaggagt acgacgaaga aggcgatgta tcgctggacg aacatctgct
     1621 cgagcgcgtc tccggtctcg aggagatcgt caagcgcagc gccgaaacga tccgcagcat
     1681 cgtcgacgcc atccagaagc acgagcgggc catctttatc aaccagaccg gcctgctctc
     1741 cgtgaaggag ttgctggagc ggaagaacgt cgtctccgcc gacgaactag tcgagctgtg
     1801 ggaatcgaag atgggcgagc agatgctcgc actcgagaag aagcagcgct ttaccgagcg
     1861 caaagaccgg atcatgtcgt tgttccgcgg tgagcggagg gagcgcttcg tacagctcgt
     1921 cgccgaggcc gagtcggcca tcgacgcgtt cgatcctgat cgcgggatca aggcgttgga
     1981 agaggcgttc aagctcgacc gcgacaacta cgagctctcc ttctttctcg gcgagatgtt
     2041 cttcaacgac ggcaatctcg accgcgccaa gcactacctc gagcgtaccc tcgaagtgca
     2101 gcccgatcat ttcgacgccg ccgttttcta cggcgtgctg cttcacgagc gccaggacct
     2161 caagggcgct gagcgctggc tgcggcgcgc gattcagatt tcgcaggagt cgttcctgcc
     2221 gtacttttcc ctcggtgcga tctacgcccg caaaggaaag ctgctgcgcg cgcagaagtt
     2281 cctcgagaag gccattcaga tcgaacagtt gccgcaggcg cactatctgc tcggcacgat
     2341 cttctacgac aaaggccagc tcgagcgcgc catcaagtcg ttccaatccg cgctgaagct
     2401 cgaccccgag tacgaggaag cgatctatca cctcggcctc tgctatctcg atcgcaactg
     2461 gaaccgcaaa gcgatcgatt gcttccagga ggcgctggag ctcaatccga acaagatcga
     2521 gtaccagcag gccgtcaaga tctacgaagg catctccggg cacatcccgc tcgaaggtcc
     2581 ggccgcggac gagttcaaga acgccgaggt gctggcgacg agcggtaaat acctcgaagc
     2641 gctcgatcac taccgtcgcg cctcggccgt cgatcccgac aacaccagca tcctcatgcc
     2701 gtacgcgctt ctctgctcgc acctcgagat gaataccgaa gcgatcgcgg ccgcgcggcg
     2761 ggtactgcgc cagcggccga ccgatgtcgt tgcagcggct gcgtacacga ccttggtcga
     2821 agcgcttcgt gccgagggaa acttcaagga tgccaatcgc gcgctggaag agatgctgcg
     2881 cgactacacc tccaattacg cgaagtcgat cgcctactac gagaaggcct acaaccttgc
     2941 ggagatggaa gagagtctcg atgaagcgct cgaatccgcg cagctcgccc ttcgctattc
     3001 acccaaagag ctgaagcagt tcccgctcgc cgccctcggc tgggtctact tcaagcgccg
     3061 cgagttcgac aacgccgtcg atttcctgac gaagtccgcc gacctcggcc cgaccgccac
     3121 aaacctcatg cacctcggca tggccctcct cgaaagcggc cagaaagacc gcgcccgcca
     3181 ggtcttccgc aaagccaaga gcttcaaagc gcagggcgcc ggcctcgaag agaagatcct
     3241 cgagcagatc cgctccacca cgaagctcat cgaccgcctg cacgcgaagc ggcggcgcac
     3301 ggcgaataag gcgtagaaag cgcggccaga ttaccgaagc ctcagcaacc tcttgtgcct
     3361 gttatcaaga attcgtgctt tggctgccac gtcctcgcag cgccgaatat cctcgtcgct
     3421 gaggactccg cgcaacgtca gtcgcttggt cacaagcttt tcgatcgaca ccacgcaaag
     3481 gcggcgaaag tcgatcaatg tctccctcgg aaagaacgga tacgaggcag gcggcagaat
     3541 caacacatcg gaaagaatgg atggagactc acgatacctg tcgaccttcg acgtggcgag
     3601 aaagtagtgg acatcgccct cttgaacggg atcagcgttg aacaccaaac acagcttgct
     3661 ccgaggtggt tcgttgtcgc cccagaaagg gtaagagtca tcatgttcgg cgcggaaaat
     3721 cacgcctcga tgaagggcgg cgattgccat cgatccgacc taccaggtca gtgactcggc
     3781 gaagtcgcga tcatcctggt cctgccgaac gagttcgatc atcggcgcgg acgggtcgaa
     3841 aaaagactca aaaggaatag ggattacgtt tcgtttcgcg gcgctgcgtg cggccaatgc
     3901 tgccccgatc tcaggaaact catgcgtcag ctcgcggagt tgccatgccg ttttcttccc
     3961 gtaaacttcg acagtctctc gcagcacttc gatgtcgcta tccgagaaaa cgtcgaggtc
     4021 cggttcatgc ctggcgatca actgggggta ggtcgaagaa tgccgcacat cgaaatactc
     4081 gcgcatggat acatcatcag gatcgtcggc aaaccgcgcg ttcatgaggt cgcgtgtagt
     4141 cgagggaact ggcccgttct ccatgcagac ataggtgtca ccggtgatcg tccgtccgta
     4201 acgtagcagg tgcgcgcgat cggcgagaaa cagcaacttc gacgtcttca tctgatcgag
     4261 gtcagccacg ccattctggg agaagaacgc gagagcattc atcaccttgt cttgatcgta
     4321 gcgaaaccgg atcgttttca taggcgtcct cggttcagag ccgacatgaa acgcacggcc
     4381 gatgcctctg gagactgatg caagaatagc actcacaaca ttgaccgcgt ataacctcat
     4441 cccgcggtga ggtagggctc atcccactgt cgacaggatt gcaaacttcg acatcccggc
     4501 tcctaacgcc gcggattcgc cagcctcacc ggtgtgatcg tttgcgcgga gatgtggata
     4561 tcgccttctt cggcgtaaag ctcgacgcgc tcgccgcctc cgttgatttt gccggttgcg
     4621 tggatgcgcg ttttggcgcc ggcctgctcg cgccggacgg tgaggccggt gaagtcgatg
     4681 tgcatctcgt tcacgtcagg atcagacgtc acgatcgtag cgtcgatgtc ggcaccgaag
     4741 cgggggctga cgatcaggtt cacattcccc tgcgtggtgc gggctcccac cttcagagtc
     4801 cgatgtctgg gatcgccagt gatggtgatg tcgccggatg tggtctgcgc gtcgatcgca
     4861 ccggatgtct gcccgacgat gatgtcgccg ccgccggaac ggagcgtcat ggggccgccg
     4921 gtgtacagca cgcgaaggag gccaccgtcg gttgaaacct cgcctcccat gcgtgcggcg
     4981 cgcacgagga tgtctcccgc gccggtgtgt gcggaaagca agccggtaat gtcgccgagg
     5041 ttcaggggac cgcccatcaa cgttaccgtg cactgtccaa atacagcgcc caactccact
     5101 tcgccggcgc cgattcgaat attcgcattg ccacgaattt caccgatagc gatatcggca
     5161 atcgatgcga cgaagggcca gtcgccgtta tgaaacggca attgaacctt taccgcagga
     5221 gccagcatca cgtcgcccgg tgtcggccgg tgctgcggcg ggttgagccg catggaagag
     5281 gcctgaccgg ggttcgatcc tttggccaac agaagaaccc gccccatcat cgtcgcggtg
     5341 ttgatcgtcg gtccgcccgg gccgttgact tgtccgcgaa aaatcgttcc cgcgaagcgc
     5401 cctccgtgaa tcggaaacgt cgtcaggatg tcgccgttga tggtgtccgc gagccattcg
     5461 aacttcgccg atgcgggcac atagacatcg atgtcggcgt tgaccgcctg cacatctgct
     5521 ttggcgatcg ggttctgctg atagtcgtag atgacccggc cgttgacgac gttgagcgct
     5581 gaggcgccgc tgacatcttc catgatcact gtgcccgcga acccgttgac cgtcacactg
     5641 ccggtgatgt gcgaaacacg aatatggtcc gctgatttgg aaccgatttt cacggcgacg
     5701 ttcttcggca cgcggatcgt gtagtcgatc gtggcgtggg cattgcgcaa tgccggcatc
     5761 accgtgcgga tcaggcggac ttttgagtcg ccgtcgaagg cgatggccac cgcatctgcc
     5821 gcttccttca gcgcgacggc gtccgaagcg acgatcgagc ggtccgccgt gaccgaaatc
     5881 aaggtgccct cgccaccgat gacgtcgatg ctgccgaagg gattgtcgat ccagacggat
     5941 ccccctggct cgatggtgag ctggcgcacg atgcggttgt ttggctggaa cgtcgtcgcc
     6001 agtaggtgtg tcgccggaaa cagcgccgcg actgcgatga gaattcgaat ggttggtttc
     6061 atcggggttg aatgctgctc agtgcctctg ccgctttgac gcgaacatag acgttttcgt
     6121 caccctgcgc cgccttcttg cgcagcgtgt cgagcatgtc gggatcgagg accgcgccgc
     6181 tgcgggcaag tttcgcgagc gcctcgactg ccttgacgcg caccgcggga ttgggatcgt
     6241 tcttgagcgc ttcgacgagc gcgtcgcgcg tgccgtcggt catcgccgac tgcatcgact
     6301 gcagcgtatc ggctgccttg atgcgcacgc cctggtgctc atcgttgcgc agcacgtttg
     6361 ccagcgcctc ggt
//
LOCUS       DATLVW010000943         5685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_40029, whole genome shotgun sequence.
ACCESSION   DATLVW010000943 DATLVW010000000
VERSION     DATLVW010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40029"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VNN25_27905"
     CDS             <1..279
                     /locus_tag="VNN25_27905"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199463.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HXH95433.1"
                     /translation="IPALFLVTFPELQALNIMRLATPQSAILSAVIFNALIIIALIPL
                     ALRGVKYRPLGAGRILRRNLLLYGVGGILIPFIGIKAIDLLLHAVHLA"
     gene            292..864
                     /gene="kdpC"
                     /locus_tag="VNN25_27910"
     CDS             292..864
                     /gene="kdpC"
                     /locus_tag="VNN25_27910"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HXH95434.1"
                     /translation="MLEHIKISLRITVVLLVITCGLYPAIVWGIGQIAFRHQANGSLI
                     ERNGKPIGSEIIGQNFAGERYFHPRPSAAGNDGYDGTVSGGSNLGPTSKKLLDTINDR
                     VTKYAATKPSGGIPADAVTASASGLDPHISPANALSQVARVARARGLSNSQIESMIHA
                     RTEGRFLGIYGEPRVNVLLLNLDLDAVPKK"
     gene            complement(949..1416)
                     /locus_tag="VNN25_27915"
     CDS             complement(949..1416)
                     /locus_tag="VNN25_27915"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXH95435.1"
                     /translation="MNGAVSTSLSEILKSDDILVNLRTETLRDAVSQLLAKPLASAGL
                     TPAAVAAAVETVMKREADGTTCIPPVALPHGRTGEVTQVVAGLGINARGVVGERPEMQ
                     IILAFVSPLDASSLHLRFLSEAARVLRQEHVIAALLSAHDGAEALAIIRAGDR"
     gene            2012..2302
                     /locus_tag="VNN25_27920"
     CDS             2012..2302
                     /locus_tag="VNN25_27920"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXH95436.1"
                     /translation="MPRAMRIDPDAARFGAILKRLREQKGWTRQKLAQRAGMTPTYIG
                     ILEYGGNTPSLPTVLELIEVLGGDIADIMRRLASARVTPAPVLPPLPPASEK"
     gene            complement(2312..3628)
                     /locus_tag="VNN25_27925"
     CDS             complement(2312..3628)
                     /locus_tag="VNN25_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HXH95437.1"
                     /translation="MTATIDVSATSEKTETLCEIRNVSHDFAMPNGSRLRVLEDINVA
                     IKPSEVVALLGPSGCGKSTILRILAGLIRPTQGEVLYHGQKVRGLTPGISIVFQSFAL
                     YPWMTVTQNVEAVLQAAGLPLDERRSVAERAIRTVGLAGFEEAYPRELSGGMKQRLGI
                     ARALSINPECLFMDEPFSHVDALTAEGLRAEVIDLWEPRDSNPSSILMVSHDIKEVVY
                     MADRIIVLASNPGRVRTIVENKLPRPRDQRSRESEALVDYLHEIITGAEMPDIPVSAV
                     ARANRIEVLPRTDTSDVIGLLEYLDAKNGVDDIFEVAAATGREFGHIMATTKAAEILD
                     LVDTPKQDIVLTAQGRRFVRAEAGERKAIWREQLLKLRLFQDVYAMLKQRPDHGANGD
                     LVREMIIMALPRENYEDMFDTLVQWARFGNLFAYEDVADWLSLQEG"
     gene            complement(3649..5388)
                     /locus_tag="VNN25_27930"
     CDS             complement(3649..5388)
                     /locus_tag="VNN25_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXH95438.1"
                     /translation="MRPREGAWGEATAFFERFRRGIWIDAVLLVGITGIVFGLSSLAG
                     EWRTLRPAVHIDLSLSALPLYTFYSLTRGLIAYFLSLLFTLTYGYWAAKDPIAERVLV
                     PLLDILQSIPVLGFMPGVVLALVSLFPNSNVGLELASVLMIFTGQAWNMTFSFYHSLR
                     SIPKDQIEASTIYRFDWWQRLKWLELPFSTIGLVWNSMMSMAGGWFFLMITESFVLGA
                     RDFRLPGIGSYMSVAVAKGDVRAMVWAIFAMITMIVVLDQLLWRPVVVWAQKFRVEEG
                     GSDEPTSTSWFYDWLRRSSVIDWISNSLAALHRRLGKHDQPVAHAQEVKRERPDASRV
                     LSFSLFTFLVIVLLFGSYRLFLLLGQLPARGYLPILGACGWTLGRVLLSTALGTIWTL
                     PAGLAIGLSPKLSRIFQPIVQIAASFPAPMLFPLAIAVLKAAGVSLGWGSVVLMLLGT
                     QWYILFNVIAGASSIPSDLGEAARSYRLSRWLRFRFLYLPAVFPYLVTGWVTAAGGAW
                     NASIVAEYVTFKGQVMTANGLGAQISLAAEHADFPRLGACVIVMAAVVVTFNRTVWKR
                     LYGLAESRFSLSK"
     gene            5475..>5685
                     /locus_tag="VNN25_27935"
     CDS             5475..>5685
                     /locus_tag="VNN25_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95439.1"
                     /translation="MPRVTDDARPDPDELLRLTKTDDKGRGRLKVYLGMAAGVGKTMR
                     MLDDAHRMRRAGVDIAIGLVETHGRE"
BASE COUNT         1181 a         1797 c         1689 g         1018 t
ORIGIN      
        1 atcccagcgc ttttcctcgt cacgttcccc gaactgcaag cgctcaacat catgcggctg
       61 gcaacgccgc agagcgcgat cctctcggcg gtgatcttca acgcgctcat catcatcgcg
      121 ctgattccgc tggcgttgcg cggcgtcaaa taccgcccgc tcggcgcagg ccgcatcctt
      181 cgccgcaacc tgctgctcta cggcgtcggc ggcatcctca tcccgttcat cggcatcaag
      241 gcgatcgatc ttctgctgca cgcggtccat ctcgcctgag gagtcatgac aatgctcgag
      301 cacatcaaga tctctcttcg catcaccgtc gtgttgctcg tcatcacgtg cggcttgtat
      361 cccgcaatcg tctggggcat cgggcagatc gcgttccggc accaggcgaa cggctcgttg
      421 atcgagcgca atggaaagcc gatcggctcc gagatcatcg gccagaactt cgccggcgag
      481 cgctacttcc acccacgtcc atccgcggcc ggcaacgatg gctacgacgg caccgtatca
      541 ggcggctcga accttggccc gacatcaaag aagctgctcg acacgatcaa cgatcgcgtg
      601 acgaagtacg cggccacgaa gccgtccggc ggaatcccgg ccgacgcagt gactgcctcg
      661 gcttccggac tcgatccaca catctcgccc gcgaatgccc tctcgcaggt ggcacgtgtc
      721 gcacgcgcgc gagggctctc gaattcccaa atcgaatcga tgatccacgc gcgcaccgaa
      781 ggccggttcc tcggcatcta cggcgagccg cgcgtcaacg tgctgctact gaatctcgat
      841 ctcgatgctg tgccgaagaa ataggaacca cagagactca gaggtcacag agaacagctt
      901 caacctctgt gtcctctgtg tctctgtggt tcagcttctt gtgtcccgct agcgatcgcc
      961 ggcccgaatg atcgcgagcg cctcggcccc atcgtgcgcc gacaggagag ccgcgatgac
     1021 atgctcctgc cgcagaaccc gagccgcttc cgacaggaag cgaagatgga gcgaggacgc
     1081 atccagcggg gagacgaacg cgaggatgat ctgcatctcg ggacgctcgc cgacaactcc
     1141 tcgggcgttg atgccgaggc cagcgacgac ttgcgtcact tcgcccgtgc gaccatgcgg
     1201 caacgccaca ggcggaatgc acgtagtgcc atctgcctcg cgcttcatca ctgtctcgac
     1261 cgccgcggca acggctgccg gcgtgagtcc cgcgcttgcc agcggcttgg ccagcagttg
     1321 cgatacagcg tcgcgcagcg tttccgtgcg gaggttgacg aggatgtcat cgcttttcag
     1381 aatctcggac agagacgtcg ataccgcgcc gttcatcgcg gttcggatcg tatcagcagg
     1441 agcaatcggg aaatgctcgt actcttacca aggcaaggat gacctctcgc gtttcttcag
     1501 acactctgcc ggcggcagca tcgatgatcg cttttcttaa acgtgcttca ccatggtagt
     1561 gaccgaatcc tcgctttcct ccgtccgttc tgctgcgcta atcgtcgatg gttgcttttc
     1621 cagcatcgac tcttaccaag gtcagcgtcg atggtcgatt ttgtaccgtg tgcgattgcc
     1681 aaggtcagca tcgagctact tttcgtacca taagctgtta ccaaggtaag catcgatcca
     1741 tcgtttcgaa ccatcgttga ttcatccgcc acctccgacg gccactgaag atcgccgaac
     1801 tgttaccttg gtatgcgcag accgtgcgaa acgaaccatc ggctctttcc aaggaaagag
     1861 cgctgggttc gaaataagca acgacgctag cctgggtaag cgcgagtgtg tcattttcaa
     1921 ccaagtttgc ttcaaatcga agcatcgatg gatgataatc gagcatcgac tctttccatg
     1981 gtttgagccg gaggtgagtt atgatgcatc gatgccacgc gcgatgcgaa tcgaccctga
     2041 tgcggcacgc ttcggcgcga tcctgaagcg gctgcgcgag cagaaagggt ggacgcggca
     2101 gaaactcgcg cagcgcgccg gtatgacacc aacgtacatc ggtattctcg agtacggcgg
     2161 caacactccc agtctcccga ccgtcctgga actgatcgaa gtcctcggcg gcgacatcgc
     2221 cgacatcatg cgccgcctcg cctctgcgcg cgtgacaccg gcacccgttc ttccgccgct
     2281 accgccggcc tctgaaaagt agctcgcggg cctagccctc ctgaagcgac agccaatcgg
     2341 cgacatcttc gtaggcgaag aggtttccga accgcgccca ctgcaccagc gtatcgaaca
     2401 tgtcctcgta gttctcgcgc ggcagcgcca tgatgatcat ctcgcgaacg agatcgccgt
     2461 tggcgccgtg atccggccgc tgtttgagca tggcgtagac gtcctgaaac agccggagct
     2521 tgaggagctg ctcgcgccag atggctttgc gctcccccgc ctcggcacgc acaaagcgcc
     2581 ggccctgagc ggtcagcacg atgtcctgtt tcggtgtgtc gaccaagtcg agaatctccg
     2641 cggccttggt cgtggccatg atgtgtccga actctcggcc ggtggcggcc gcgacttcga
     2701 agatgtcgtc gacgccgttc ttcgcatcga gatactcgag cagaccgatg acgtccgaag
     2761 tatccgtgcg cggaaggact tcgatgcggt tggcccgcgc aaccgccgag acaggaatgt
     2821 cgggcatctc ggcgccggtg atgatctcgt gcagatagtc gacgagcgcc tccgattcac
     2881 gagaccgctg atcgcgcggc ctcggcagtt tgttctcgac gatcgttcga acgcgtccgg
     2941 gattgctggc caggacgatg atgcgatcgg ccatgtacac cacttctttg atgtcgtgac
     3001 tgaccatgag aatcgacgac ggattcgaat cgcgcggttc ccacaggtcg atcacttcgg
     3061 ccctcaaccc ctcggcagtc agcgcatcga cgtggctaaa cggctcgtcc atgaacagac
     3121 actcgggatt gatggagagc gctcgggcga tgccgaggcg ctgcttcatg ccgcccgaca
     3181 gctcgcgcgg ataggcttct tcgaatcctg cgagaccaac ggtacgaatg gcgcgctcgg
     3241 cgaccgatct ccgctcgtcc agcggcagac ccgccgcctg cagcaccgcc tcgacgttct
     3301 gcgtgaccgt catccacggg tagagcgcga agctctgaaa gacgatcgag atgccgggcg
     3361 tgaggccgcg aaccttctgc ccgtgataca gaacctcgcc ctgggtcgga cggatgagtc
     3421 ccgcgagaat gcgaaggatg gttgatttgc cgcagcccga gggtccaagg agcgcaacca
     3481 cttccgacgg tttgattgcg acgttgatgt cctcgagcac acgaaggcgg gagccattcg
     3541 gcatcgcgaa gtcgtgcgac acattgcgga tttcgcagag ggtctccgtc ttttcagaag
     3601 ttgcggaaac atcgatcgtc gctgtcatcg gtcacctcac ctgcgccatt acttactcag
     3661 ggaaaatcgg gactctgcca gtccgtacag ccgcttccag accgttcgat taaacgtcac
     3721 aaccaccgcc gccatgacga tgacgcaagc cccgagccgc ggaaaatcgg cgtgctcggc
     3781 ggcaagactg atctgcgcgc cgagtccgtt cgcggtcatc acctggcctt tgaacgtcac
     3841 gtactcggca acgatgcttg cgttccatgc gccgcccgca gccgtcaccc aacccgtcac
     3901 gagatacgga aacactgccg gaagatagag aaatctaaag cgaagccagc ggctcagccg
     3961 gtaactgcgc gccgcttcgc caagatccga ggggatggac gaggcaccgg cgataacgtt
     4021 gaagaggatg taccactgcg tgccgaggag catgagaacg acgctgcccc atccgaggga
     4081 gacacccgcg gccttcaaca ctgcgatcgc gagcggaaac agcatcggcg ccggaaacga
     4141 agcggcgatc tgcacgatcg gctgaaagat ccgcgatagc ttaggcgaaa ggccgatggc
     4201 gagaccggcg ggaagcgtcc agatcgtccc gagggcagtc gagagaagca cgcgtcccag
     4261 cgtccaacca caagcgccga ggatcggcag gtagcctcgt gcaggcaatt gcccgagcag
     4321 caggaacagg cgatagctac cgaagagaag aacgatcacg aggaaggtga agagcgaaaa
     4381 cgacaatacg cgcgaagcat ccgggcgctc tcgcttcact tcctgcgcat gcgccactgg
     4441 ctgatcgtgc ttgcccaggc ggcggtgaag agcggcgagt gagttgctga tccagtcaat
     4501 gacgctcgat cgtcgcagcc agtcgtaaaa ccacgacgtc gacgtcggct catccgagcc
     4561 gccttcctcg acacgaaatt tctgcgccca gacgacgacc ggccgccaca gcagttgatc
     4621 aaggacgacg atcatcgtga tcatcgcgaa gatcgcccac accatcgcgc gaacgtcgcc
     4681 cttcgccacg gcgacgctca tgtacgagcc gataccggga aggcggaagt ccctggcgcc
     4741 gaggacgaac gactcggtga tcatcaggaa aaaccagccg ccggccatgc tcatcatgct
     4801 gttccagacg aggccgatcg tggagaacgg caactcgagc cacttcagcc gctgccacca
     4861 gtcgaaacga tagatggtcg acgcttcgat ctggtctttc ggaatcgatc ggagcgagtg
     4921 gtagaaactg aaggtcatgt tccacgcctg gccggtgaag atcatgagca cggaggccag
     4981 ctccaggccg acgttgctgt tcgggaagag cgaaacgagc gctagaacga cgcccggcat
     5041 gaagccgaga acgggaatgc tctgaagaat gtcgagcagg ggtacgagga cgcgctccgc
     5101 gatgggatcc tttgccgccc aatagccgta cgtcagcgta aagagcagcg aaaggaagta
     5161 cgcgatcagg ccgcgtgtga gcgaatagaa ggtgtagagc ggaagcgcgg agagcgagag
     5221 atcgatgtgg acggcaggcc ggagcgtgcg ccactcgccg gctagcgaag agagtccgaa
     5281 gacgatgccg gtgatgccga caagcaggac ggcatcgatc cagatgccgc gccggaagcg
     5341 ctcgaagaac gcggtggctt cgccccatgc tccttcccgc ggccgcatcg tattgaccct
     5401 gtctccatca ctcgcatgct ctcacactcg gaaccttcgc gcagccacaa agctcggtca
     5461 ggtattctga gcgcatgccc cgcgttaccg acgatgcccg tccagatccc gacgagctgc
     5521 tgcgcctcac gaaaaccgac gacaagggac gcggccggct gaaggtctac ctcggcatgg
     5581 ccgccggcgt cggcaagacg atgcgcatgc tcgacgacgc ccatcgcatg cgccgcgccg
     5641 gtgtcgacat cgccatcggt ctcgtcgaga cgcacggccg cgagg
//
LOCUS       DATLVW010000944         5317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_577115, whole genome shotgun sequence.
ACCESSION   DATLVW010000944 DATLVW010000000
VERSION     DATLVW010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577115"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VNN25_27940"
     CDS             <1..764
                     /locus_tag="VNN25_27940"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXH95440.1"
                     /translation="SVGKRTGAYSSGGAYDVHPFMLINYNGRYEDMSTVAHELGHTMQ
                     SYFSNKTQPYPTAGYPIFVAEVASTFNEALLNEYMLKQIKDPATRLSLLGSYLENMKG
                     TVFRQTQFAEFELRIHEAAEKGEPLTGDTFDKIYYDIVKRYYGNDQGICIVDDVVKHE
                     WAYIPHFYRTYYVYQYATSGTASAALSEKVLSGDASATKRYLAFLSAGGSKYPIDLLK
                     DAGVDMTTSEPLDLTMKKLNRVMDEMEAILKSMGK"
     gene            complement(909..1502)
                     /locus_tag="VNN25_27945"
     CDS             complement(909..1502)
                     /locus_tag="VNN25_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95441.1"
                     /translation="MKRIRIAHALFLTLITIAFMSCSSDSDDRDRGPRSYPGAAEGGG
                     RPGGFARAGAGDGLDTMPPADWWKDPKISVAVALSADQIASLDRISHEQAEEIAKLER
                     DSMVAARELRETFDGSQPATADIVAAGQRMRGIRDALFDRRVQMFAAERTLLTQQQWQ
                     TLQQQIAANNRPERGDRGNNGPRGGRGGRGGRGRFPG"
     gene            1601..2677
                     /locus_tag="VNN25_27950"
     CDS             1601..2677
                     /locus_tag="VNN25_27950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95442.1"
                     /translation="MRSTIVAVLTLFIASAVLAQWEPPDAPKDLPDTGGTPRVYFAHS
                     YFPKAPKTAGLDDRLSLHIQNFSTLLKAVNGNCQGIVLFLDGMPIKGIKPESCDPVLG
                     HVRYRLLRTPDDEAAWHTLLGSPKGYVNPIEASVGSNAQFPIATSARFNLEVIPRPQL
                     LVFITLLFLALCVMIYLCRTTSLLRSGTHPVPSERPYSLSLFQMAFWFFLVIAAYVFM
                     WLITDELDTITDSILALIGIGAATALGATLIDRTKTAPAPDEPGGKSRGFINDVISDP
                     TGVSLHRFQMFVWTLVLGVIFVGSVYKNLEMPEFSATLLGLMGISSGTYLGFKVPEKQ
                     GSDTTPGATPDAEPVKPPEPPVKQ"
     gene            complement(2841..3212)
                     /locus_tag="VNN25_27955"
     CDS             complement(2841..3212)
                     /locus_tag="VNN25_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXH95443.1"
                     /translation="MAVEEEDRLYKIGEVCKVAEVQPYVLRYWETEFPPLAPNKSGGG
                     QRLYTRHEIDIILRIKQLLYSEGFTIAGAKKRLEIEMASPAPIAASEPSVQSTVQSKS
                     TDKALGDVKRELTAILRILDE"
     gene            complement(3232..4281)
                     /gene="waaF"
                     /locus_tag="VNN25_27960"
     CDS             complement(3232..4281)
                     /gene="waaF"
                     /locus_tag="VNN25_27960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HXH95444.1"
                     /translation="MASETVVVGLNWVGDNVLALPTYRALYHRYRAEGGITVAAPKHI
                     ASLLASAGIFRNVIAWNGDTRERIRLLKAGRFRRAVILPNSFRAAMVAFAAGIGERWG
                     YSTDVRGMLLTHGVRRDPARGHQLDDYSKLLAAMNAPRVVDEIPTMSIPIHLREQAGR
                     QLRDAGVRLDNGPLFGLHAGGLYGRAKHWGDERYGQLAERLQAAGFSVVLLTSPGEKH
                     QAAAISATTRGLPIVGGDGDVLQLAAAISHCSVVVTNDSGPLHLATALAVPSVSIFGP
                     TDPDRTVIPGASRVVRKPFACQPCYKRDCPLMHHGCMNEISVDDVFAAAVGLFNELDG
                     KIETDGAGAVAFGTE"
     gene            complement(4271..5203)
                     /locus_tag="VNN25_27965"
     CDS             complement(4271..5203)
                     /locus_tag="VNN25_27965"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXH95445.1"
                     /translation="MSRRRKNALLQRVEYLFYRAIAGAVRSASLSGLRRWGSRLGTLA
                     RHVLRGRDRLAMRNLVATFPEKSIAECRAILDECWRHFGREALAAIRMQHVPIEEIPS
                     HCRFVNEHILEDAIAQGRGVLLLSAHYGAWEIAGLAIVALVKNVHTVARPLDNELLER
                     DLAFIRARTGVQVVDRKGAARSLLKALSQKGVIVLLPDQAVQAREGILVPFLGRPAWT
                     TDAPAKLALRLQSPIVFGFCIADEAGHKLEFEEAIRIDQLDDDEKNATALTERINDVI
                     SRRIEARPELWLWMHDRWKGNASGEIEVKTTDGQ"
BASE COUNT         1004 a         1669 c         1621 g         1023 t
ORIGIN      
        1 tgagtgttgg caagcgcacc ggcgcctact ccagcggcgg cgcgtacgac gtccatccgt
       61 tcatgctgat caactacaac ggccgctacg aggacatgag caccgtcgca cacgagctcg
      121 ggcacacgat gcagagctac ttctcgaaca agacgcagcc gtatccgacg gccggctatc
      181 cgatctttgt ggccgaggtg gcgtcgacgt tcaatgaggc gctgctgaac gagtacatgc
      241 tcaagcagat caaagatccg gccacgcgcc tctcactgct cggcagctat ctcgagaaca
      301 tgaaggggac cgtgttccgc cagacacagt tcgcggagtt cgagctgcgc attcacgaag
      361 cagcggagaa gggcgagcct ctcaccggcg acacgttcga caagatctat tacgacatcg
      421 tcaagcgcta ctacgggaac gatcagggca tctgcatcgt cgacgacgtc gtgaaacacg
      481 aatgggcgta catcccccat ttctaccgca cgtactacgt ctaccagtac gcgacgtcgg
      541 gtaccgcatc ggccgcgctg tcagagaaag tgctctccgg cgacgcaagc gccacgaagc
      601 gctacctggc tttcctctcg gccggcggat cgaagtaccc gatcgacctg ctgaaagatg
      661 ccggcgtcga catgacgacg tcggagccgc tcgacctgac gatgaagaag ctcaatcgcg
      721 tgatggacga gatggaggcg attctgaaat cgatgggcaa gtgatttgtt ttgcgcggcc
      781 tggcggccgc gcgttgtcgg ttgtcggttg attgcgcctt cggcgcgttg tcgattgccc
      841 agcttcgcta actgacaacg cgcgaagcgc aatcaaccga caacgcgcgc agcgcaataa
      901 agcagcgcct atcccgggaa ccgtccccgc ccaccgcgcc cgccccggcc tccgcgaggg
      961 ccgttgttgc cgcggtctcc gcgctcgggg cgattgtttg cggcgatctg ctgctggagg
     1021 gtctgccact gttgctgcgt caggagcgtt cgttcggcgg cgaacatctg cacgcgccgg
     1081 tcgaagagcg catcgcggat gccgcgcatc ctctgaccgg ctgcgacgat gtccgcggtt
     1141 gccggctggc tcccgtcgaa cgtctcgcgc aactcgcgtg cggcgaccat gctgtcccgc
     1201 tcgagcttcg cgatctcctc ggcctgttcg tgactgatgc gatcgagcga tgcgatctga
     1261 tcggccgata gggcaacggc aacgctgatc ttcggatctt tccaccagtc cgcgggcggc
     1321 attgtgtcca gaccatcgcc tgcgcctgcc cgcgcgaagc cgccgggcct ccctcctccc
     1381 tcggcagcgc ctggataact gcgagggccg cggtcgcggt catcagagtc cgaagagcag
     1441 ctcatgaagg cgatggtgat gagcgtgagg aagagggcgt gcgcgatgcg aatgcgtttc
     1501 atgtccccag cctacgacct ccgcttcgat ctgttgagcc gtagtctgtc ctttgtctta
     1561 gaatcgttgc agtccacaaa tccggaaaag cgaggtgcgt atgcggtcga cgatcgtcgc
     1621 ggttctcacg ctgttcatcg ccagcgcggt gctggctcag tgggagccgc ccgacgcacc
     1681 gaaggatctt ccggacaccg gcggcactcc gcgcgtttat ttcgcgcatt cctactttcc
     1741 caaagccccg aagacagccg gcctcgatga ccggctttcg ttacacatac agaacttctc
     1801 gacgctcctc aaggccgtga acggaaactg tcagggcatc gttctctttc tcgatggaat
     1861 gccgatcaaa ggaatcaagc cggagtcatg cgacccggtc ctcgggcatg tgcgctaccg
     1921 cctcctgcga acgccggacg acgaggccgc gtggcacacg ctcctcggga gtccgaaggg
     1981 ctatgtgaac ccgatcgaag ccagtgttgg ctccaacgcg cagttcccga ttgcaacctc
     2041 agcaaggttc aatctcgaag tcatcccacg tccgcaactc ttggttttca tcacgttact
     2101 ttttcttgca ctgtgcgtca tgatctatct ctgccgcacg acaagtttgc ttcgcagcgg
     2161 cacgcatccg gtgcccagcg agcgtcccta ctccctctcg ctgttccaga tggcgttctg
     2221 gttctttctc gtcatcgccg cctatgtctt catgtggctg atcaccgacg agctggacac
     2281 gatcaccgac tcgatcctcg cgctcatcgg catcggcgct gctacggccc tcggcgcgac
     2341 gctcatcgac cggaccaaga cggcgcctgc accagacgag ccgggcggca aatcgcgagg
     2401 cttcatcaac gatgtgatca gcgatccgac cggcgtcagc ctgcatcgtt ttcagatgtt
     2461 cgtctggacg ctagtcctcg gagtgatctt cgtcggatcc gtctacaaga atctggagat
     2521 gccggagttc agcgccacgc tgctcggact catgggaatc agctccggca cgtacctcgg
     2581 cttcaaagtt ccggagaagc aaggtagcga cacgacgccg ggtgcgaccc cggacgcgga
     2641 gccggtcaaa ccgccggagc cgccggtgaa gcagtgatga gtggagagaa tcagagacaa
     2701 gagatcagag acaagagatc aggaggcggt ctccttgaac tctgatctct gatctccaaa
     2761 tctctggtct ctagtctctg aatctctggt ctctgatctc cgaatctctg atctctgatc
     2821 tctgatctct ggtttctgac ctactcgtcg aggatccgca gaatcgcggt cagctcacgc
     2881 ttcacatcgc ccaacgcttt atcggtgctt ttcgactgca ccgtcgattg cactgacggc
     2941 tccgaggctg cgatgggggc cggcgacgcc atctcgatct cgaggcgttt cttggcgccc
     3001 gcgatcgtga agccctccga atagagaagc tgtttgatgc ggaggatgat gtcgatctcg
     3061 tggcgggtgt agaggcgctg gcctccgccg ctcttgttgg gcgccagcgg tggaaactcc
     3121 gtctcccagt atcgaagcac gtacggctgc acctcggcga ctttgcaaac ctcaccgatc
     3181 ttgtacagcc ggtcttcctc ttccacagcc atcccgaacg attatagggc gctactcggt
     3241 gccgaaggcc accgcgccgg ccccgtcggt ctcgatcttc ccatcgagct cgttgaaaag
     3301 gccgaccgcg gcggcgaaaa cgtcgtcgac gctgatctcg ttcatgcagc cgtggtgcat
     3361 caacggacag tcgcgtttgt agcaaggctg gcaggcgaac ggtttgcgga cgacgcgcga
     3421 ggcgccggga atcacggtcc ggtcgggatc ggtcggcccg aagatcgaca cggatggcac
     3481 ggcgagggcg gtggccagat gcagcggacc gctatcgttc gtgacgacga ccgaacaatg
     3541 cgaaatggca gcggccagtt gcaggacgtc gccgtcgccg ccgacgatgg gtagaccgcg
     3601 ggtcgttgcg gagattgctg ctgcctgatg cttctcgccg ggacttgtca gcagaacgac
     3661 cgagaagccg gcggcctgca gacgttccgc gagttggcca taccgctcgt cgccccaatg
     3721 tttggcgcgt ccgtaaaggc ctcccgcgtg caggccgaat agagggccgt tgtcgagccg
     3781 aacgccggcg tcccggagct ggcggccggc ctgttcgcga agatggatcg gaatcgacat
     3841 cgtcggaatc tcatcgacga cgcgcggtgc attcatcgct gcaagcagct tcgaatagtc
     3901 gtcgagctgg tgccctcggg cggggtcacg ccgtacgccg tgagtgagca gcatgccgcg
     3961 cacgtcggtc gagtagcccc accgttcgcc gatgccggcc gcaaacgcca ccatcgcggc
     4021 gcggaatgag ttcggaagga tcacggcgcg gcggaagcgg ccggccttga gcaatcggat
     4081 gcgctcccgc gtgtcgccgt tccaggcgat gacgttgcgg aagatgcctg ccgaggcgag
     4141 gagcgaggcg atgtgcttgg gcgcagcgac cgtgatgccg ccctcggcgc gatagcgatg
     4201 gtacagcgcg cggtacgtgg gcagagcaag gacattgtct ccaacccaat tgagtccgac
     4261 tacgactgtc tcactggcca tctgttgttt tgacttcgat ctcacccgat gcgttcccct
     4321 tccaccggtc gtgcatccat aaccataact ccgggcgtgc ttcgatgcgc cgggaaatta
     4381 cgtcgttgat acgctccgtc agtgctgtgg cgtttttctc gtcatcgtcc aattgatcga
     4441 ttctgatcgc ttcctcgaac tccagcttgt gcccggcttc gtccgcaatg caaaaaccga
     4501 acacaatcgg cgattgcagt cgcagcgcca acttcgcggg ggcatcggtc gtccatgccg
     4561 gacggcccag aaacggaacg agaatccctt cacgagcctg caccgcctga tccggaagga
     4621 ggacgatgac ccccttttgc gagagcgctt tgagtaaaga gcgcgcggcg cccttgcggt
     4681 cgacgacctg tacgccggtg cgagctcgaa tgaaagcgag gtcgcgctcg aggagctcgt
     4741 tgtcgagcgg cctggcgacg gtatgcacat tcttcacgag ggcgacgatc gccagcccgg
     4801 cgatctccca cgcgccatag tgggcactca ggaggaggac tccacgtccc tgcgcgatcg
     4861 catcctcgag aatgtgctcg ttgacgaagc ggcagtgcga cgggatttcc tcgatcggga
     4921 catgctgcat ccgaatggcc gccagcgcct cgcggccgaa atgccgccag cactcgtcga
     4981 ggatcgcgcg gcactccgcg atgctcttct ccggaaacgt cgccacgaga ttgcgcatcg
     5041 ccagccggtc acggcctcga aggacgtgcc gggccagagt gccgaggcgc gatccccagc
     5101 ggcgcagacc agagagggat gccgagcgaa ccgcgccggc aatggcccga taaaacagat
     5161 actcgacgcg ctgcagaagc gcgtttttgc ggcggcggct catggtgggt tgaattagag
     5221 gatcagagat caggaaatca gagacaagag atcagagatc aggacgcagg gtgatgatct
     5281 cactcatcat cgccactgat tccctgatct caatccc
//
LOCUS       DATLVW010000945         4891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_330750, whole genome shotgun sequence.
ACCESSION   DATLVW010000945 DATLVW010000000
VERSION     DATLVW010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330750"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VNN25_27970"
     CDS             <1..689
                     /locus_tag="VNN25_27970"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXH95446.1"
                     /translation="ILHPSTGYAVHDFAEAWTLVRATRLRTRWSDAIAAVCAAAIIAL
                     IWRLADAPVTAWAERVQSSGIQDTIRFANRFGGGMNPPMVVLFFLIAGVAYRYHRWIA
                     YAVAMAFSGAAAGVAAQIIKYAAGRTRPELWLGPFQHARAAATSFPSGHTVGAFALGG
                     VLMLASPSRTMRVAAFLLALSVAVSRVLAFRHWTSDVLASAAIGILIAAVVVRGVNVT
                     SGVAAEAQSE"
     gene            complement(823..1422)
                     /locus_tag="VNN25_27975"
     CDS             complement(823..1422)
                     /locus_tag="VNN25_27975"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXH95447.1"
                     /translation="MKTTMRSLLSILILVSAAACVNADELAIGAKAPQFSLVNAVDGK
                     TVAFHPGDGKVSVVVFTCNQCPYAKAFEPRIIELARQYQAKGIAFYAIDSNDESQYSE
                     ESLPEMKSRATERGYPYPYLKDGNSAIAHAYGARVTPHVYVIDGRGTLRYRGYVDDSA
                     KPEERRTTGLANALDELIAGKTVSNQTTRAFGCTIKFKA"
     gene            complement(1419..1886)
                     /locus_tag="VNN25_27980"
     CDS             complement(1419..1886)
                     /locus_tag="VNN25_27980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXH95448.1"
                     /translation="MLALLLLATTASAADLHDVASPPRINAFFPRHAKIRVVNIWATW
                     CVPCVAEMPDLRMINAAFGPEVTMIGVSMDDMIPGAKRENVARFLDQHWIGYLNVYYK
                     GNADDLSNYYHFEGEIPVTIAFDAAGHEIWRHQGPIKSSLTIAELRKLLRRMR"
     gene            2195..3202
                     /locus_tag="VNN25_27985"
     CDS             2195..3202
                     /locus_tag="VNN25_27985"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXH95449.1"
                     /translation="MTIIVLFGGRSDERHVSVASAQNVVRALDAPLAWFWAPNGAVHD
                     VHRDDLLHHENPFTNDFEPSRPAIWPDLEQALDTLPVEDPVFLLALHGGEGEDGGVQR
                     LMEARGIPFTGSGSAASAAAFDKGRAKEMVENHLRVAESLVVDDFRAIDGSIATMLKR
                     HEKIVLKPVAGGSSRGLFFVDQTTDLAPVVAAIHALKVPYILEQFIAGRELTVGVIDS
                     ENGPRALPVIEIETDPGFSFDYEGKYLGKGTREICPARIPDAIRDEAQQMALTAHSVL
                     GCEGYSRSDLIFADDGLYFLELNTLPGLTSTSLVPQQLREAGVSFRDFLEEQVAIGRR
                     R"
     gene            complement(3281..3910)
                     /locus_tag="VNN25_27990"
     CDS             complement(3281..3910)
                     /locus_tag="VNN25_27990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXH95450.1"
                     /translation="MVAIRFTGNYEDLSTDRGYQFKFYCEKCNNGYMSSFNTSTVGVL
                     SSAARVAGSLFGGVFGNVANSSYEVQRAIGGPAHDSALKEAVDEISPIFKQCTRCGHW
                     VCEPVCWNKKAGLCENCAPDLDEELAAAQAEAAREQVQTKAREVDWTKSRQIDQVSGA
                     VCGSCGAKSQGGKFCPECGTAYSAKKKCASCGHEAEGTPKFCPECGGKY"
     gene            4095..>4891
                     /locus_tag="VNN25_27995"
     CDS             4095..>4891
                     /locus_tag="VNN25_27995"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXH95451.1"
                     /translation="MRKYLAICLFFISALPLVAQQPAPLKIPRVSQHQVITQTVGLTD
                     ITIDYSRPVAKGRVIFGGLVPYDQVWRTGANEATQITFSDDVTIDGKPLPKGAYSLHT
                     IPGKDSWTLIFNKTAKQWGSFNYEQAQDALRVTAKPQKGPFAEVFTITFPDVTTDDAV
                     VALRWADTMVLFKVGTNTTANVMAAATAAVAGAAPTDAATPARAASFAFDSGNNALAM
                     KWIDQSLKIKPSMGNLYLKSRIQAKGGDRAGAIATAEGALKMATPKD"
BASE COUNT          867 a         1556 c         1529 g          939 t
ORIGIN      
        1 tgatccttca tccgtcgacc ggttacgccg tgcacgattt cgcagaggcg tggacgctcg
       61 tgcgggccac gcgcctgcga acgcgctgga gcgatgcgat cgccgccgtt tgcgcggcgg
      121 cgatcatcgc gctcatctgg agactcgccg atgcgccggt cacggcctgg gccgagcgcg
      181 tgcagtcgtc ggggattcag gacaccatcc gttttgccaa ccgtttcggc ggcggcatga
      241 acccgcccat ggtcgtcctc ttctttctca tcgccggcgt ggcctatcgc tatcaccgct
      301 ggattgccta cgccgtggcg atggcgttca gcggcgcggc agccggcgtc gccgcccaga
      361 tcatcaaata cgccgccggc cggacgcgcc ccgagctctg gctcggcccg ttccagcacg
      421 cccgcgccgc cgcgacatcc tttcccagcg gccacaccgt cggcgccttc gccctcggcg
      481 gcgtgctgat gcttgcctcg ccctcgcgca cgatgcgcgt cgccgccttc ctcctggcgc
      541 tctccgtcgc cgtctcgcgc gtgctcgcct tccgccactg gacgagcgac gtcctcgcct
      601 ccgccgcaat cggcatcctg atcgcagcgg tggtggtgcg gggagtgaac gttacaagcg
      661 gcgtggccgc cgaggcgcag tcagagtgag cgatcgctcg ccgtctggtt aagaaccttg
      721 tcacgcggag acgcggagtt gcggagaaag cactccgcgt tctccgcgtc tccgcgtgaa
      781 accgtctccc caataatcaa tcagggaaag tgtatttgct gatcacgcct tgaacttgat
      841 cgtgcaaccg aacgcgcgtg tggtctggtt cgacaccgtc ttcccagcga tcagctcatc
      901 gagggcgttg gcgaggccgg tcgtgcggcg ctcttcgggc ttggcgctgt cgtcgacgta
      961 accgcggtag cggagcgtgc ccctgccgtc gatcacgtag acgtgcggcg tgacccgcgc
     1021 tccgtacgcg tgggcgatcg cgctgttgcc atccttgagg tacggatacg gataacccct
     1081 ctccgtcgcg cgcgacttca tctccggcag cgactcttcc gaatactgcg actcatcgtt
     1141 cgaatcgatg gcgtagaacg cgattccctt cgcctgatac tgcctggcca gctcgatgat
     1201 gcgcggctcg aacgctttcg cgtaggggca ctggttgcag gtaaagacga cgaccgagac
     1261 cttaccgtcg ccgggatgaa acgccacggt cttgccatcg acggcgttca cgagcgagaa
     1321 ctgcggcgct ttggcgccga tcgccagctc atcggcgttc acgcacgcgg cagcggaaac
     1381 gagaatcaga atcgaaagaa gtgacctcat ggtggttttc atcgcatcct cctgagcagc
     1441 ttgcgcagct cggctattgt taacgaactc ttgatcgggc cctgatggcg ccagatctcg
     1501 tgtccggcgg cgtcgaaggc aatcgtgacg gggatttccc cttcgaaatg gtaatagttg
     1561 gagagatcgt cggcgttgcc tttgtagtaa acattgaggt agccgatcca gtgctggtcg
     1621 aggaagcggg ccacgttctc gcgtttcgcg cccgggatca tgtcgtccat cgacactccg
     1681 atcatcgtca cttccggacc gaacgcggca ttgatcatgc gcagatccgg catctcggca
     1741 acgcagggga cgcaccacgt cgcccagatg ttcacgacgc ggattttggc gtgccgagga
     1801 aagaaggcgt tgatccttgg gggagaggcg acgtcgtgca gatcggccgc cgaggcggtg
     1861 gtcgcgagga ggagcagggc tagcatcagc gggttagttc gcagcggcat atgcgcgggc
     1921 aacgcgttgt gcgattgtga tattcgacgg cggagatgaa cgggagtcgg gagtcgggag
     1981 tcggagcaaa gctgcacggt cccaacgctc gcgcgatacg ctgctccgac cccgggcacg
     2041 cgacgacgac ggggatgaac ggggagtcgg gggtcgggag tcggagaaaa agctggcgat
     2101 caacgcgata cgctgccctt ctccgacccc cgacccccga ctcccgaccc ccgactcccg
     2161 cacccacgca tgccgcttgc acaatggaca cgcaatgaca atcatcgttc tcttcggcgg
     2221 ccgcagcgat gagcggcacg tctcggtagc atcggcgcaa aacgtcgttc gcgcgctcga
     2281 cgcgccgctg gcctggttct gggctccgaa cggcgccgtg cacgacgtcc atcgcgacga
     2341 tctgctgcat cacgaaaacc ccttcacgaa cgatttcgag ccgtcgcggc cggccatctg
     2401 gcctgacctc gagcaagccc tcgacacgct tccggtcgag gaccccgtct ttcttctcgc
     2461 gcttcatggc ggtgaagggg aggacggcgg cgtacaacgt ttgatggagg cgcgcgggat
     2521 tccgttcacc ggctccgggt cggcagcaag cgccgcggcc ttcgacaagg gacgggcgaa
     2581 ggagatggtg gagaatcatc tgcgcgtcgc cgaatcgctc gtcgtcgacg acttccgcgc
     2641 gatcgatggc agcatcgcca cgatgctcaa gcgccacgag aagatcgtgc tgaagccggt
     2701 cgccggcggt tcgtcacgcg gtctcttctt cgtcgaccag acaaccgatc ttgctccggt
     2761 cgtggcggcg atccacgcgc tgaaagttcc ttacatcctc gagcagttca tcgccggccg
     2821 cgagctgact gttggtgtca tcgatagcga gaacggaccg cgtgcgctgc cggtgatcga
     2881 gatcgagacc gatcccggct tttccttcga ctacgagggc aagtaccttg gcaaaggaac
     2941 ccgcgagatc tgtcccgcca ggatccccga cgcgatccgc gacgaagcgc agcagatggc
     3001 gctgaccgcg cacagcgtgc tcggatgcga ggggtactca cgtagcgatc tgatcttcgc
     3061 ggacgacggg ctctactttc tggaactgaa tacgctgcca ggactcacga gtacttcgct
     3121 tgtaccgcag cagttgcggg aggcgggtgt gtcgtttcgc gattttctgg aagagcaggt
     3181 ggcgattggg cgaaggcgtt agtcacgcga agaagcgaag acgcgaagga gatattgact
     3241 ccttcttctt cgtgtcttcg cgtcttcgcg tgagaagttg ttaatacttc cccccacact
     3301 ccggacagaa cttcggcgtt ccctcggcct catgaccgca cgaggcgcac tttttcttcg
     3361 cagagtatgc cgtgccgcac tcgggacaga acttgccgcc ctgcgacttc gctccgcacg
     3421 atccgcagac cgcgccggag acctgatcga tctggcgcga cttcgtccag tccacctcgc
     3481 gcgcttttgt ctgtacctgt tcccgtgccg cctcggcttg cgctgccgcc agctcctcat
     3541 ccaggtccgg tgcgcagttc tcgcagagtc cggctttctt gttccagcag accggctcgc
     3601 agacccaatg tccgcagcgc gtgcactgtt tgaagatcgg cgagatctca tcgaccgctt
     3661 ctttcagcgc gctgtcgtgc gccggaccgc caatggcgcg ctggacctcg tacgaactgt
     3721 tggcgacgtt tccgaagacg cctccgaaca ggcttccggc cacgcgcgcg gcggaactga
     3781 gaacgccgac ggtcgaggta ttgaaacttg acatgtaacc gttgttgcac ttctcgcagt
     3841 aaaacttgaa ctgataaccg cggtccgtcg ataggtcttc gtagtttccg gtgaagcgga
     3901 tagcgaccat gtgttctcct tggtgttcgg ggtgtgggga gtagggggtg gggagtggga
     3961 gcaaaggcag agtgaagcgc atatctacaa cgcttgcgct tttggttctg ctcccactcc
     4021 ccacacccca ctccctacac cccgacattt tgctacgatc cggccctggt tacgaaaccg
     4081 aaaggagctt ttcgatgcgc aagtaccttg cgatttgtct tttcttcatc tccgcgctgc
     4141 cgctggtagc gcagcagccg gcgccactca agattccgcg cgtgtcccag catcaggtca
     4201 tcacgcaaac ggtcgggctc accgacatca cgatcgatta cagccgcccc gtggccaagg
     4261 gccgcgtaat cttcggcggt cttgttccgt acgaccaggt gtggcgcacc ggcgcgaacg
     4321 aggcgaccca gatcaccttc agcgacgacg tgacgatcga cggcaagccg ttgccgaagg
     4381 gcgcgtattc gctgcacacg atccccggga aggattcgtg gacgctgatc ttcaacaaga
     4441 cggcgaagca gtggggctcg ttcaactacg agcaggcgca ggacgcgctg cgcgtgaccg
     4501 cgaagccgca gaagggaccg tttgcggagg tgttcacgat cacgtttcct gacgtgacca
     4561 ccgacgacgc cgtagtcgcg ctgcgctggg ccgacacgat ggtgctcttc aaagtcggca
     4621 cgaacacgac agcgaatgtg atggcagcgg cgactgctgc cgtcgctggc gccgctccga
     4681 cggatgcggc cactcccgcc cgcgcggcat cgttcgcatt cgactccggc aacaacgcgc
     4741 tggcgatgaa atggatcgat cagtcgttga agatcaagcc gtcgatgggc aatctctacc
     4801 tcaagtcgcg aatccaggcg aagggtggcg accgcgcggg agcgatcgcg accgccgagg
     4861 gcgcgctaaa gatggccacg ccgaaagacg a
//
LOCUS       DATLVW010000946         6217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_330795, whole genome shotgun sequence.
ACCESSION   DATLVW010000946 DATLVW010000000
VERSION     DATLVW010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330795"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VNN25_28000"
     CDS             <1..1021
                     /locus_tag="VNN25_28000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95452.1"
                     /translation="VDARVTYATDSNDFTMKRLDLEGNRWVSQRWDADLSLGRHEFEH
                     AGDSVYASTISVGASYRYTDRTTARGRVGLDFYDSGLGTRPYFALGAEFMPSLQSRAS
                     VDFNHYDLVYDVFTLTSLGTPGPTSQLNLRDPLSINDFRGHYDWNGGGFFSWLADGSY
                     GFVSDNNKRAGAHGLATFRVLKSPFLAFKVDGRYLAYDFRTNRYWSPNDYRSIAGVVQ
                     IGQTVRQKFRWDFEVKAGKAYESGFSSDLRAYEGNITVPLSDSFDLIGNYGYGKSGRL
                     DSVFAGSTSSSDFVNYWQRHWFVGVRAKQLFARASERHPHDQYYYDARPLSESPVVPP
                     LGGTN"
     gene            1021..2340
                     /locus_tag="VNN25_28005"
     CDS             1021..2340
                     /locus_tag="VNN25_28005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH95453.1"
                     /translation="MATVKKPLRPATQLIISIAIIALVVIATLFLFRYPQSLGPIFIT
                     ALVVTLASFLLLLVLRHFVLIWFSYLHQRELADQPPAESYPFVSIVVPAYNESEVIQA
                     SLSSLLELRYPYYEIIAVDDGSTDGTFDKMKEFEGNHYGVKVSVYRKENSGKADTLNY
                     GIRRSRAPVVVCMDSDSRLTPDALRYAIRPFSDPNVGAVAGNVKVINRHNIWTKLQAL
                     EYIEGLNIVRKAQAYFRTVNVIPGPIGIFRRSAFDATGGYDSDTFAEDFDMTVKILAD
                     GWKIYYEPKAVAYTEAPEELLDVIKQRYRWSRGILQALRKQKRLLVSRGSSVTTPLSL
                     WYMVFEGLVWPAMNIFASVFFVWIALIYGMTKLLVLWWMLLTALDLLIAIHAIVMEGE
                     QMSLAPYAIFYRFFYITIIDVTKVFATLEELVGLDMSWGKLARKGRI"
     gene            2344..2529
                     /locus_tag="VNN25_28010"
     CDS             2344..2529
                     /locus_tag="VNN25_28010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95454.1"
                     /translation="MRFDWVNILATVIFVSIVVTLILAVASYFAYKVREARRPKTAAE
                     LRQDGETKVFFTQYVPK"
     gene            2595..3656
                     /locus_tag="VNN25_28015"
     CDS             2595..3656
                     /locus_tag="VNN25_28015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95455.1"
                     /translation="MPRHHDAHVHWKHLASYGAVMFLVAAIGAFALWTFFEKQSLVVV
                     PDPLPKITVVTVDANSRLAAAWVNLLTRSGLQATLVPMEKFEPVEGVVVICDVPALPP
                     KLASMLEAFVRRGGSLAFAGTPPSTPIGKLLLNAEVGPSDTAIKLSESASPILARLNP
                     GSEVQMRRSSVALLKESPQMIVDARWQEKARAAVMHMEQQGARYLWMGFDPDAMVEKD
                     DRQMLLLIRTAFRWAAGQPVSQGAVGEAATAKTLTPESRRDARAANFAFGVDRLANRR
                     FFLLHMANRGPKPIDNPSVMIWLPPNTTDVTLGGDFFMRRNVSLTAMQGERAWVVSVP
                     RLGPNEERVIKLKVETGAR"
     gene            3798..5489
                     /locus_tag="VNN25_28020"
     CDS             3798..5489
                     /locus_tag="VNN25_28020"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXH95456.1"
                     /translation="MNPEVKDLQPKLKEVLYDCAVEIRATKAALFLYDSVGRYEIVAE
                     YGFKGAIRQSVDQKDPVVDRCGRGRSAFFVNGLSAEPRFSEIMYESASDRLLAAPIYN
                     RGQLVGLIDMRDKAQKQLFDNADIPKAQKIAERIVALFAGKNVFGQRFISLSHPDDPQ
                     NVVPRSTIQAAPLPSAAADVARVPVSSSVKSTSPAPATPAKASIEPGARVPRLSALII
                     DGRAAANRIVLPPSPESLTENELAAVRDVLRSILLIPSVAVVSFTAFGHTGGIQEMAA
                     KSVISEEALAFLQSKLNVWLAKRGESGGHTRRNVQLPLGPGTASIQSSQMVKVFTAPV
                     TAGAFRSLYLTVAFNANPDRVTHELLAAMLTQLQVAIERSVDRTSLQSLRERAAIALL
                     EPDFTKYPELRTHCDRVSSRAESLARYLEMSPSEIENVKLAALVHDVGMRLLDYDRLY
                     RKPDISLDEIHLLREHTTVGAALVEPILGHEVARAVLCHHERWDGTGYPNELAGGEIP
                     RIARIIHVCDVYESMISTESYKPAQPRAAALAMIAENGGTQFDAAIANRFIEMVR"
     gene            5778..6155
                     /locus_tag="VNN25_28025"
     CDS             5778..6155
                     /locus_tag="VNN25_28025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95457.1"
                     /translation="MADCPEFVIWNPLKPGWKFFAQASAATSTGTASDDFDASVRIVP
                     PAGSPADWVKADLNPGPESIDSLEAGGYGASARLVSGPSGPTVTLRAWIVDSEGAKPF
                     DCTWTNSDAGSEFHISIMLVVKA"
BASE COUNT         1148 a         1953 c         1882 g         1234 t
ORIGIN      
        1 ggtcgacgcc cgcgtgacgt acgcgaccga ctcgaacgat ttcacgatga agcgcctgga
       61 tctcgaaggc aatcgctggg tgtcgcaacg atgggatgcc gatctgtcgc tgggccgtca
      121 tgagttcgag cacgccggcg acagcgtcta cgcgagtacg atcagcgtgg gtgcgtcgta
      181 tcgctacacg gaccgcacca ccgcccgcgg tcgcgtcgga ctcgacttct acgacagcgg
      241 actcggtacc cgcccctatt ttgcactcgg cgccgagttc atgcccagcc tccagagccg
      301 cgcatcagtc gacttcaatc attacgacct cgtctacgac gtcttcacgc tgacctccct
      361 cggcacgccc ggtcccacgt cgcagctcaa tctgcgcgat ccgctctcca tcaatgactt
      421 tcgcggtcat tacgactgga acggcggcgg tttcttctcc tggctggcgg acggttcgta
      481 cggcttcgtc tcggacaaca acaagcgcgc cggcgcgcac ggcctggcaa cgttccgtgt
      541 cctgaagtcg ccgttcctcg ctttcaaagt ggacggccgc taccttgcgt acgacttccg
      601 tacgaatcgc tactggtctc cgaacgacta ccgatcgatc gccggtgtgg tgcagatcgg
      661 ccagaccgtg cgccagaagt tccgttggga cttcgaagtg aaagccggga aggcgtacga
      721 gtccgggttc agctccgacc tccgcgccta cgaaggaaac atcaccgtgc ccctcagcga
      781 ctcgttcgat ctgatcggca actacggcta cggcaagagc ggcaggctcg actctgtctt
      841 cgcagggagc acgagctcga gcgatttcgt gaactactgg cagcgtcact ggttcgttgg
      901 tgtgagggcg aagcagcttt tcgccagggc cagcgagcgc catcctcacg accagtatta
      961 ctacgacgcg cggccgctca gtgagtcgcc cgtcgttccg ccgctgggag gaacaaacta
     1021 atggcaaccg tcaagaagcc gcttcgcccg gcaacgcagt taatcatctc gattgcgatc
     1081 atcgccctcg tggtgatcgc cactctcttc ctgttccgct atccgcagtc actgggaccg
     1141 attttcatta cggcactcgt cgtgacgctt gcctcgttcc tgctgctgct ggtgctgcgg
     1201 catttcgtgt tgatctggtt cagctatctg caccagcgcg agctcgccga tcagccgccc
     1261 gcggagtcgt atccgttcgt gtcgatcgtc gttccggcgt acaacgaatc cgaggtcatt
     1321 caggcgtcgc tctcgtcgct gctggagctc cgctatccgt actacgagat catcgcggtt
     1381 gatgacggct cgacggacgg cacgttcgac aagatgaagg agttcgaagg gaaccactat
     1441 ggcgtgaagg tttccgtcta tcggaaggag aacagcggaa aagcggacac gctcaactac
     1501 gggatccgcc gctcgcgtgc tcccgtcgtc gtctgcatgg acagtgattc gcggctcact
     1561 cccgatgcgt tgcgttatgc gatccgtccc ttctccgacc cgaatgttgg cgccgtggcc
     1621 ggaaacgtca aggtcatcaa ccgtcacaac atctggacga agctgcaggc gctcgaatac
     1681 atcgaaggtc tcaatatcgt ccgcaaggcg caggcgtact tccgaacggt caacgtgatt
     1741 ccaggaccaa tcggcatctt ccggcgctcc gcgttcgacg cgacgggagg ctacgacagc
     1801 gacacgttcg cggaagactt cgacatgacg gtcaagatcc tcgcggacgg ctggaagatc
     1861 tattacgagc cgaaggcggt tgcttatacc gaggcgccgg aggagctgct cgacgtcatc
     1921 aagcaacgct atcgttggag ccgcggcatt ctgcaggcgc tgcgcaaaca gaagcggctg
     1981 ctcgtctcgc gcgggagcag tgttaccact cccctctcgc tctggtacat ggtgttcgaa
     2041 ggcctcgtat ggccggccat gaatatcttc gccagcgtct ttttcgtgtg gatcgcactc
     2101 atctacggca tgacgaagct gctcgtgttg tggtggatgc tgctgactgc tctcgatctg
     2161 ctcatcgcca ttcacgccat cgtgatggag ggcgagcaga tgagtcttgc gccgtacgcg
     2221 attttctacc gtttcttcta catcaccatc atcgacgtca caaaggtttt cgccacgctc
     2281 gaggagctcg tcgggctcga catgtcgtgg ggaaagctcg ctcggaaagg acgcatctaa
     2341 atcatgagat tcgattgggt caatattctt gcaaccgtca tcttcgtttc gatcgtcgtc
     2401 acgctgattc tcgccgtcgc gtcctacttc gcgtacaagg tgcgcgaggc acgccgtccc
     2461 aagaccgccg ccgaactgcg ccaagacggc gagacgaagg tgttcttcac ccagtacgtg
     2521 ccgaaatgac gtgctggatg cctgtgctga ccatgatgtg cggtcagcac aggactcgtt
     2581 cgcagcactc ctgaatgccc cgtcatcatg acgcacacgt tcattggaaa cacctcgcca
     2641 gctacggcgc ggtgatgttc ctggtggcgg cgatcggcgc gttcgccctc tggacctttt
     2701 tcgaaaagca atcgctggtg gtcgtgcccg atccgctgcc gaagatcacg gtggtgacgg
     2761 tcgacgccaa ttcgcggctg gcagcggcgt gggtgaatct gctcacgcga tcgggactgc
     2821 aagccacgct cgtgccgatg gagaagttcg aaccggtcga aggcgtggtc gtcatctgtg
     2881 acgttcccgc actccctccg aagcttgcct ccatgctgga ggcctttgtg cgccgcggtg
     2941 gctcgctcgc attcgccggt accccgccgt cgacgccaat cggcaaactg cttctgaacg
     3001 ctgaggtggg accgtcggac acggccatca aattgtcgga gagcgcctcc ccgatcctgg
     3061 cgcggctcaa tcccggctcc gaagtgcaga tgcggcgttc ctcggtcgca ctattaaagg
     3121 agtcaccgca gatgatcgtc gacgcgcgct ggcaggagaa ggcgcgcgcg gcggtaatgc
     3181 acatggagca gcaaggcgca cgctatctgt ggatgggctt cgatcccgac gcgatggtgg
     3241 agaaagacga tcgccagatg ttgttgctca tccgcaccgc gtttcgctgg gcggcaggac
     3301 aaccggtctc ccagggggcg gtgggagagg cagcgacggc gaagacgctc acgcccgaat
     3361 cacggcgcga cgcacgcgcc gcaaacttcg cattcggcgt cgatcgtctg gcgaaccgtc
     3421 gcttctttct ccttcacatg gcgaaccgcg ggccgaagcc gatcgacaat ccgagcgtga
     3481 tgatctggct gccgccgaac acgaccgacg tcaccctcgg cggagacttc ttcatgaggc
     3541 gcaatgtttc gctgaccgcg atgcagggcg aacgcgcgtg ggtggtgtcg gtgccgcgtc
     3601 tcgggccgaa tgaagagcgt gtcatcaagc tgaaggttga aacgggggcg cgctgagttt
     3661 gaaaaacgga tcaccacaga gacacagagg tcacagagat cccctctgtg ttctctgtgt
     3721 ctctgtggtt gcctttgctt cggcgacaac gactacccaa taaccacatt gccgcctccc
     3781 gccttacaat ctcgcgcgtg aatcccgagg taaaggatct tcagccgaaa ctgaaggaag
     3841 ttctttatga ctgcgcggtg gagatccgcg ctacgaaggc cgcgctcttt ctctacgaca
     3901 gcgtcggacg ctacgagatc gtggccgagt acggattcaa gggcgcgatc cgccagtcgg
     3961 tcgaccagaa ggatccagtc gtcgaccgct gcggccgcgg acggtcggcg ttcttcgtca
     4021 acggcctcag cgccgagccg cgcttctccg agatcatgta tgagtcggcc agcgaccgcc
     4081 tcctcgccgc gccgatctac aaccgcgggc aactcgtcgg gctgatcgac atgcgcgaca
     4141 aagcgcagaa gcaactgttc gataacgccg acatcccgaa agcgcaaaag atcgctgagc
     4201 gtatcgtcgc gcttttcgcg ggcaagaacg tcttcgggca gcgcttcatc tccctttcgc
     4261 accccgacga cccacagaat gtcgtgccgc gctccacgat tcaggcggcg ccgttgccgt
     4321 cggcggccgc ggacgtggcg cgcgttcctg tttcttcatc ggtcaagagc acctcgccgg
     4381 ctccggccac gcctgcgaaa gcatccatcg aaccaggcgc ccgtgttcct cggctctccg
     4441 cattgatcat cgacggccgc gccgcagcaa accgcattgt gctgccaccg tcgccggaat
     4501 cgctgaccga gaacgagctc gccgcagtcc gtgacgtgct ccgatcgatt cttctcattc
     4561 cgagcgtggc cgttgtctcg ttcaccgcgt tcggacacac gggcggcatc caggagatgg
     4621 ccgcgaagtc cgtaatctcg gaggaggcgt tggcgtttct gcagtcgaag ctcaacgtct
     4681 ggctggccaa gcgcggcgag tcagggggac acacgcgcag gaatgtgcag ttgccgctcg
     4741 gacccggcac ggcatcgatc cagtcctcac agatggtgaa ggtgttcacg gcgcccgtga
     4801 cggccggagc gttccggtcg ctttatctga ccgtggcctt caacgcgaac cccgaccgcg
     4861 tcacgcacga actgctcgcg gcgatgctga cgcagttgca ggtggccatc gaacgatcag
     4921 tggaccggac gtcgctgcag tcgctgcggg agcgcgcggc tatcgcgctg ctcgagcccg
     4981 acttcacgaa ataccccgag ttgcgcactc actgcgatcg ggtgtcgtcg cgcgccgaat
     5041 cgctggcgcg ctatctggag atgtcgccga gtgagatcga aaacgtgaaa cttgcggcgc
     5101 tcgttcacga tgtggggatg cgactgctcg actacgaccg cctctatcgc aagcccgaca
     5161 tctcgctcga cgagatccac ctgctccgcg agcacaccac ggtcggcgcc gccctggtcg
     5221 agccgatcct ggggcacgag gtcgcccgcg ccgtgctctg tcatcacgaa cgctgggacg
     5281 gcaccggcta tccgaacgag ttggccggcg gcgagatccc gcgcatcgcg cgcatcattc
     5341 acgtgtgcga tgtctacgag tcgatgatct cgacggagag ctacaagccc gcgcaaccgc
     5401 gcgccgccgc cctggcgatg atcgccgaaa acggcggcac gcagttcgat gccgcaatcg
     5461 cgaaccggtt catcgagatg gtgcggtagg gggcggatgg ggagtgggta gtagggagtg
     5521 gggggtggga gccggcggtg tcgctagtgc tgcgaataac tggactctcg cgcaattacc
     5581 aaattcaatc ggtcttaagt taagttgtgt cagtatcttg cctcgttcca acgaccgaag
     5641 gtctggcccc catgctcgga gttgcggcgg atctcttcgc gtccttcgcg tcttcgcgtg
     5701 aaatgcttct tgtcctttcc cttagaatgc cgcgtccgct catccgtccc actcctctat
     5761 gaaagggttg aatcgtcatg gctgattgcc cggagtttgt catctggaac ccgttgaaac
     5821 ccggctggaa gttcttcgcg caagcgagtg ccgccacgag caccgggacg gcgagcgatg
     5881 acttcgatgc cagcgtccgt atcgtgccgc cagccggcag cccggccgac tgggtcaaag
     5941 cggatctcaa tccaggaccg gagtccatcg actcactgga agccggcggc tacggcgcaa
     6001 gcgcgagact cgtcagcggt cccagcggcc ccacggtcac gctgcgcgcc tggatcgtcg
     6061 attccgaagg cgcaaagcca ttcgactgca cgtggacgaa ttcggacgcg ggatccgagt
     6121 ttcacatctc gatcatgctg gtggtgaaag cgtgatgaac agagtcatca agcttctcag
     6181 tgcgtcgctg ttggtgatgg ccttcgccgc aagcgcg
//
LOCUS       DATLVW010000947         4524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_577236, whole genome shotgun sequence.
ACCESSION   DATLVW010000947 DATLVW010000000
VERSION     DATLVW010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577236"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1309)
                     /locus_tag="VNN25_28030"
     CDS             complement(254..1309)
                     /locus_tag="VNN25_28030"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXH95458.1"
                     /translation="MRKFALVIALFAAVAVHAEQKNVQLLTNLSDWQLNAVMDNFTAS
                     LGVHCDACHVRNAETKQWDFPSDAKGEKKTAREMIRLVLDLNEKSFHGHTVVGCYTCH
                     RGKESPSLMVALPVPPIPPTKSEAEETAERKSYPAAKDVVAKYVTAIGGEAAAKKLST
                     ATMTAKASRVGGNGQTMPIEVYRSGGKFLQRGTPAEGPAMVQMYGPEGGWMTGRQGAH
                     TITGGDAAIAVASARAYEPFDGSLSDKARVQGKETIDGHETWIVGAPIDEHTRQRLYF
                     DATTGLVVRRVVSMDSPVGRLPTQTDFDDYRDVAGVKVPFTVKVASVNGGQSATRKYT
                     AIEFGAPVDEKMFEAPK"
     gene            1396..2430
                     /locus_tag="VNN25_28035"
     CDS             1396..2430
                     /locus_tag="VNN25_28035"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXH95459.1"
                     /translation="MADRVEVHIPFTTLLKVALTLLLCICVIKLWPVILMIIVAILLA
                     VMLDPIVTFLERHRVHRGLGVAIVAIVLLALLVVVFALLVPAMSRQLVDLSKTLPQVE
                     QSISQRFPAVAPYLKSLHGGPPPELETWLTRGIAAGKYAIELVTAMVFVLIVALYLLI
                     EGRTVFEWLITLAPKNKRPRLRRTAVEMAAVMLAFTRGQAICCSICATWTFLLLTLLH
                     IPAAVPLAGMAFIADLVPVVGTIAMMIPAVMLALTAGPVKALLVFAGFLLYHVAESYF
                     IIPKVYGSELRLSTLTVILAIMIGGTLQGVAGAILILPFVAAYPIVERLWLRDVLPED
                     AVARHEELAE"
     gene            2540..2965
                     /locus_tag="VNN25_28040"
     CDS             2540..2965
                     /locus_tag="VNN25_28040"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXH95460.1"
                     /translation="MKRKASAVWNGSLKEGKGTISTESGLLSNAQYSFGTRFEDGKGT
                     NPEELIAAAHAGCFSMALSGQLTAAGTPPSKVATSATVTLEKLEAGFTVTSVHLDVVA
                     SVPGATQEGFDTAAGNAKKGCPISRLLNAEITMTARLES"
     gene            3441..3647
                     /locus_tag="VNN25_28045"
     CDS             3441..3647
                     /locus_tag="VNN25_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HXH95461.1"
                     /translation="MPEQGTVKWYNDAKGYGFIVRERGADVFVHHASIIAEGSKSLAE
                     GDKVSFDVVEGPKGLQAKNVVKIS"
     gene            complement(3772..>4524)
                     /locus_tag="VNN25_28050"
     CDS             complement(3772..>4524)
                     /locus_tag="VNN25_28050"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXH95462.1"
                     /translation="FYIDIGAHDPVVGSVTQHFYLSGWAGINVEPLPAFFECLVAARP
                     NDINLNVAVSQNAGTLDLIVDHTEPGLSTMTQELADEYARAGHRLERIEVPTRPLADI
                     VREHCAGRTVDFLKIDAEGHELEILSGIDLSVWRPRIILIEAGYKPEAWAPMIVSAGY
                     ILAGSDLWNRYYVRTEDAERADLLRCPANVTDRYIAFDHVRALQAQAEWDSFGPLTRR
                     IARRLHRLLNANPRFKGAVKKVAQRIGLLRDS"
BASE COUNT          820 a         1385 c         1389 g          930 t
ORIGIN      
        1 gtggggtgtg gggagtggga gatcgatgct ttcggcgggt gtcggtgcgc gggagtcggg
       61 agtcggagca gtcgaaatca ggatgctcga tacgtgcgtg ggagcgccca atcgcagttg
      121 ggatgagagc gggtttcaat tacaacaccc gacgaccgac tcccgacatt ctcaatggag
      181 cgaaggagag aagggtgcag cgcgaggggt ttggctacca ccccccacgc cccacacccc
      241 actcccatat tcactatttc ggcgcttcga acatcttctc gtccacaggt gctccgaact
      301 cgatcgccgt gtacttccgc gtcgcgctct ggccgccgtt gacgctggca accttgaccg
      361 tgaacggcac cttcacgccg gccacatcgc gataatcatc gaaatccgtc tgggtcggaa
      421 ggcggccgac ggggctatcc atcgacacga cgcgcctcac gacaaggccg gtggtcgcat
      481 cgaaataaag ccgctgccgt gtatgctcgt cgatcggagc accgacgatc caggtctcat
      541 gtccgtcgat cgtttccttc ccctggacgc gggctttgtc ggaaagtgat ccgtcgaacg
      601 gctcgtacgc gcgcgccgag gcgacggcga tcgcagcgtc cccgccagtg atcgtgtgag
      661 ctccctgccg tcccgtcatc catccaccct cggggccgta catctgcacc atggccggac
      721 cttctgccgg cgtgccgcgc tgcaggaact taccgcccga gcgatagacc tcgatcggca
      781 tcgtctgtcc gtttccgccg acgcgtgagg ctttggctgt catcgtcgcg gtggagagtt
      841 tcttcgccgc ggcctcgcca ccgatggcgg tgacatattt cgcgacgacg tctttcgcgg
      901 cgggatacga cttacgctca gccgtttcct cggcttcgga cttcgtgggc ggaatcggag
      961 gaaccggcag cgcgaccatc agcgacggac tctcttttcc gcggtggcac gtgtagcagc
     1021 cgacgacggt gtgaccgtga aagctcttct cgttgaggtc gaggaccagg cggatcattt
     1081 cgcgcgcggt cttcttttcc cccttcgcgt cgctggggaa atcccactgt ttcgtctctg
     1141 cgttacggac gtgacaggcg tcgcagtgaa cgccgaggga ggcggtgaag ttatccataa
     1201 ctgcgttgag ctgccagtcg gagaggttcg tcagcaactg cacgttcttc tgttccgcgt
     1261 gaaccgcgac tgcagcaaat aaagcaatga ccagggcgaa tttccgcatg aatgctcctt
     1321 cgtttttccg ttgcgcgacg gaggatagcg caaccccctc cgcagcggcg tctgttgtgc
     1381 attacgtaac acaccgtggc cgaccgcgtc gaagtacaca tcccgttcac cactctgctg
     1441 aaagtggcgc tgacgctcct tctctgcatt tgcgtgatca agctttggcc ggtgattctg
     1501 atgatcatcg tcgcgattct gctggccgtg atgctcgatc cgatcgtcac gttcctcgag
     1561 cgtcaccgcg ttcaccgcgg cctcggcgtc gcaatcgtgg ccatcgtcct gctcgcgttg
     1621 ctcgtcgtag tgttcgccct tttggtacct gcgatgagcc ggcaactggt ggacttgtcg
     1681 aagacactgc cgcaggtcga gcaatcgatc tcccaacgct tcccggccgt ggcgccctat
     1741 ctcaagagtc ttcacggcgg cccgccgccg gaactggaga cctggctgac tcgcggaatc
     1801 gcggccggaa agtacgccat cgagctcgtc acggcgatgg ttttcgtgct catcgtcgcg
     1861 ctctatcttc tgatcgaggg acggacggtc ttcgaatggc tcattaccct cgcgccgaag
     1921 aacaaacgtc cgcggctgcg gcggaccgcg gtcgagatgg ctgctgtgat gctcgcattt
     1981 acccgcggcc aggccatctg ctgctccatc tgcgcgacct ggaccttcct cctgctgaca
     2041 ctgctccaca ttcccgccgc ggtcccgctg gcaggcatgg ccttcatcgc ggacctagtg
     2101 ccggttgtcg gcacgatcgc catgatgatt cccgccgtga tgcttgcgct gaccgcgggt
     2161 cctgtgaagg cgctcctggt cttcgccggt ttcttgctct atcacgttgc cgagagctac
     2221 ttcatcatcc cgaaggtcta cggatcggag cttcgccttt cgacgcttac cgtgattctg
     2281 gcgatcatga tcggagggac gctgcagggc gtcgccggtg cgatcctgat cctcccgttc
     2341 gtcgcggcct atccaattgt ggagcgcctc tggctccgcg acgttctacc cgaggacgcc
     2401 gtggcccgcc acgaagagtt ggcggagtaa caggagtgcg gtcgtctcga ccgcgggttg
     2461 actttcgaat cgaatagccc ggcgtcgccg ccctttcctc ggtctacaat cgccgccact
     2521 caaaaccaag gagcaattga tgaaacggaa agcatccgca gtttggaacg gaagtctgaa
     2581 ggaaggaaaa ggaacgatct cgacggagag cgggctgctc agcaatgccc agtattcgtt
     2641 cggaacgcgc ttcgaagatg ggaaggggac gaatcccgag gaattgatcg cggccgcgca
     2701 tgcaggctgc ttctcgatgg cgttgtcggg acagctcacc gccgccggca cgccgccgtc
     2761 gaaggtcgcc acgagcgcaa cggtgaccct ggagaagctg gaagccggct tcaccgtgac
     2821 gtcggttcat ctcgatgtcg tcgccagcgt ccccggcgcg acgcaggaag gtttcgatac
     2881 ggccgccggc aatgccaaga agggttgtcc gatctcgcgc cttctgaacg ccgagatcac
     2941 gatgacggcg cgcctggaat cgtagtgcgt cgggggtcgc gagtcggggg tcggagcaga
     3001 accggcggcg ctagccgctc gaacgttctt gggagctgcc ggacgtcgcg agcgcctctt
     3061 cctgtttctc tagagtgctt tcccatcgag cgccttctcc ccccgcgctt tttgcggggg
     3121 gagaaggtgc cgaaggcgga tgaggggggc gtcaatgcgc ggggcgatca accacgcctc
     3181 acccgaaccg cctgtaaacg cgtcagatgc acggatgtcc tacttgttgg agcgccccct
     3241 catccggcct tcggccacct tctcccccac aagaagcgtg ggggagaagg cgctcgatgg
     3301 gaatggtcgc gataagagtg tgagaaaaac gagttagccc tggttagcga ccccagctcc
     3361 gactcccgac tcccgacacc tggtatccgt tgacgcacgt catttgcggc gctaacgtag
     3421 ctccaattag tgaggtacgt atgccggaac agggaaccgt caagtggtac aacgacgcga
     3481 agggctacgg tttcatcgtg cgggagcgcg gcgcggatgt gtttgtgcac cacgcttcga
     3541 tcatcgccga ggggtccaag tcgttggcgg aaggcgacaa ggtgtccttt gacgttgtcg
     3601 aagggcccaa aggattgcag gcgaagaacg ttgtgaagat ctcgtagcga gggtggcggg
     3661 cggtgcggtg aggcagagat tagaggcgag agactagaga tcagggcatt gtcagggcac
     3721 acccatctga tcccttgtct cttatctcct atctcttgcc tctgattcac ctcaggaatc
     3781 gcgcagtagc ccgattcttt gcgcaacctt cttaaccgcg cccttaaacc gcggattggc
     3841 attcaatagc cggtgcagac ggcgtgcgat tcgccgagtg agcggcccga acgaatccca
     3901 ctccgcctgc gcctgtaatg cgcgaacgtg atcgaacgcg atgtagcgat cggtgacatt
     3961 cgccgggcat cgcagcagat ccgctcgttc tgcgtcttcg gtgcggacgt agtaacgatt
     4021 ccagagatcg ctgccggcaa ggatgtatcc ggcacttacg atcatcggag cccacgcttc
     4081 gggcttgtag ccggcttcga tgagaatgat ccgaggacgc cagacagaga gatcgatccc
     4141 gctcagaatc tccagttcgt ggccttctgc gtcaatcttg agaaaatcga ccgtgcgtcc
     4201 ggcgcaatgt tcgcgcacga tatcggcgag gggacgcgtc ggaacctcga tccgttcgag
     4261 acggtgcccg gcgcgcgcat actcatccgc gagctcctgc gtcattgtgc tcagtcccgg
     4321 ctccgtatga tcgacgatga ggtcaagcgt gcctgcattc tgcgacacgg ccacattgag
     4381 attaatgtcg tttggccgcg cggccacgag gcactcaaag aacgccggca atggctcgac
     4441 gttgatcccg gcccatcctg ataagtaaaa gtgctgcgtc acggaaccga ccaccggatc
     4501 gtgcgcgccg atgtcgatgt agaa
//
LOCUS       DATLVW010000948         6574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_411275, whole genome shotgun sequence.
ACCESSION   DATLVW010000948 DATLVW010000000
VERSION     DATLVW010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411275"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VNN25_28055"
     CDS             <1..582
                     /locus_tag="VNN25_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXH95463.1"
                     /translation="RREMTEPAGPSEITELLKEWSEGKDEVLDQLMPLVYAELRRQAA
                     RHLRRERRDHTLQTTGLIHEAYLKLIGQKNVQWESRTHFFAIASQAMRRVLVDYARAR
                     HRAKRGGDDVRISITAAGEIASETKPVDLIALDEALTRLAAIDEQQSRVVELRYFSGL
                     SLEETAAILNISRATAAREWSMAKAWLHRELSR"
     gene            592..3642
                     /locus_tag="VNN25_28060"
     CDS             592..3642
                     /locus_tag="VNN25_28060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXH95464.1"
                     /translation="MDADRWQEIKRVFEGAMEQAPGQRLDYLTEACQGDETLLQEVNS
                     LLRSLDDAHSFMEQPAVGEVAGQITASPVHDYSKGQSVAHYRIISELGAGGQGTVYKA
                     LDTKLGRTIALKLLPPEMTSVDDTVRKRFRREAQLASSLDHPNICTVHDFIEADGASF
                     IIMQFVAGSTVRQLVGGRPLAISTALRIAIQVCDALAAAHSKGIIHRDIKAQNIIVSE
                     NGQAKILDFGIAKLIGEGSDGKDQTEITALGSPYGTPTYAAPEQSRGEKVDHRADIFS
                     TGVLLYEMFSGTWPFQGRTAIEARHAVLYDEPRPISENRGGPIPAKLEAIVSRALSKE
                     PAHRYQRMADMRDALIEVLRELPESKEDSAAGFLEGFRPSTLRHSPRLNWKFGLTAAA
                     LLVVVAAILAYGLYSRHRASTAIGKKDTILITDFTNTTGDAVFDDTLKQGLAMQLQQS
                     PFLNIFPGARVRETLRQMERSPDERVTVETGREICQRNDLKALIAGSIAALGRHYVIT
                     LEAINAKSGETIGRQQTEADSKEEVLKSLSSAASGIRETLGESLSSIRQSDIPLYQLT
                     TPSVEALKSFALGFDLSNRGEYFKSIPMFKRSVELDANFAYGYSLLAGNYTIVNEPRR
                     AAENAAKAFALKDKGTDRERLYITSVYDNYTLGDLDKAIEAFRVYDQFYANDFRASGN
                     LSLAYLLLGQYEKALGEARESVRLNPGISAWQVTLGTSLLRLNRFRDAKETFDRAIVM
                     GLNDARMHDALYQLAFVQHDEKGMQQQLDWARGRPEEYVAAGLQSAAAAYRGQWRESQ
                     TLTQRAIDLAVHVDVKEVAARYAAEEGLRAAVLGRCDQAKVYAEQSLALDHNQVTQER
                     IALSQALCGDRRAQSSLQGDVQQRPGDTLLNGLWVPVVRAASELNDGNAEKAVASLDN
                     TRTYEPAAEFWTHYIRGEAELKLNRGTDAASEFRQILSHAGEAPLSILYPLAQLGLAR
                     ATAQTGARSQSLKAYQDFLNSWKDVDPDLPIVRSARAEYAKLNR"
     gene            complement(3707..6004)
                     /locus_tag="VNN25_28065"
     CDS             complement(3707..6004)
                     /locus_tag="VNN25_28065"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HXH95465.1"
                     /translation="MKRFVCALLLLLAATALTAAIQTPSQFLGFEVGADRKLADYRQI
                     VSYFHALAAASPRVKVESLGKTTLGEDFIMAVVTSEGNMRNLEKIRETARKLADPRGL
                     SDEQISQLAREGKSIVLVTCNIHSSEIASSQMAMEWVHALASANDAETKRRLDNVVLL
                     LVPSLNPDGQIMITDYYRKYLGTRYEGGRLPWLYHHYVGHDNNRDWFMLTQLETKELS
                     RAVYHEWYPQVWVDEHQMGTDGPRMFIPPFADPLDPTVHPLIWREANLVGTNMAFRLE
                     QQEKAGLIYGFSFDAYWLGGTRNTGWWKNITGLLLETASARIASPIVIDPSELRGGQK
                     GLIDYKATINHPHPWAGGTWRMRDIMDYERIASDALLETAADRREDLLRDVAVRARAA
                     VAFGATSAYRIPHDQHDWPTAQFLAALLLEHGVEIQQAGNGDYWISMAQPYSRFLDEL
                     LEPQRYPEVRLQPGKDILRPYDVASWTLPLAMGVTVEKTVMPPKTGLQQVTSIKPETE
                     EVIKRHASQPKPRIAIYNPWGGALDEGWTRWLLDSYGFTPKSLHPQEVKAGLQNFDVF
                     IIPDIDKEEVATGRRAGGEGSMKYIDELPPENRGALEKEGAQTLRTFVENGGTLIAFN
                     AGCDYVIDNFNIPVRNVLAKATPNDFSVPGSLARVHIRTDHPVTAGMPDETAVFIDHP
                     IAFETTLPGNEMQRWVLATYPDDARDVLLSGWINGEEKLTRKAAAVAMTFGKGRIVLF
                     GFRPQHRGQTHATYPMVFNALYWGR"
     gene            complement(6015..6362)
                     /locus_tag="VNN25_28070"
     CDS             complement(6015..6362)
                     /locus_tag="VNN25_28070"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXH95466.1"
                     /translation="MTRHRDIVPLGGMLDTSHRLMSITENKSRADFDADENLQYALTH
                     LLQRIGRNAERVSDAVRTQHPEIDWPHVASLQTRVSLDYKTIIHDAVWFGATVEVPRL
                     LTALEAFMPSDPP"
     gene            complement(6346..>6574)
                     /locus_tag="VNN25_28075"
     CDS             complement(6346..>6574)
                     /locus_tag="VNN25_28075"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXH95467.1"
                     /translation="GSVVRDDFDPERSDVDVLVELRPDTTATGFAFFGAQEELSQIFG
                     RKVDMSTPAALSRWMIKDVLTEAVEEYDQTS"
BASE COUNT         1302 a         1931 c         2092 g         1249 t
ORIGIN      
        1 cgcagagaaa tgaccgagcc tgccggccca tccgagatca ccgagctgtt gaaggagtgg
       61 agcgagggga aggatgaggt gctcgatcag ctcatgccgc tcgtctatgc ggagctgcgg
      121 cgtcaggcgg cgcggcactt acggcgcgag cggcgggatc acacgctgca gaccaccggg
      181 ctgattcacg aggcctacct gaagctgatc ggccagaaga atgtgcaatg ggagagccgc
      241 acgcatttct ttgccatcgc ctcgcaagcc atgcgccgcg ttctggtgga ttacgcacgg
      301 gcgcgacatc gggcgaaacg gggcggagac gatgttcgga tctccatcac ggcagccggg
      361 gagatcgcca gcgagacgaa gccggtcgat ctgatcgcgc tcgacgaagc cttgacgcgc
      421 ttggcggcaa tcgacgaaca gcaatcgcgc gttgtcgagc tacgctactt cagcggcttg
      481 agtctcgaag agaccgcggc gattctgaac atctcacgag cgaccgccgc gcgcgaatgg
      541 agcatggcca aggcctggct gcatcgcgaa ttgtcgaggt aacgctgcgc aatggacgcc
      601 gatcgctggc aggaaatcaa acgagtgttc gagggcgcca tggagcaggc tcccggccag
      661 cggctcgact atctcaccga ggcctgccag ggcgacgaga cgctcctgca ggaggtcaat
      721 tcccttcttc gctctctcga cgacgcgcac agtttcatgg agcaaccggc agtgggagaa
      781 gttgcgggtc agatcactgc gagcccggtt cacgattact caaagggtca gagcgtcgcg
      841 cattatcgaa tcatttccga gttgggcgcc ggaggtcagg gcaccgtcta caaggcgctg
      901 gacacaaagc tgggccgcac gatcgcactc aagctgctgc cgccggagat gacctccgtt
      961 gacgacactg tccgcaagcg atttcgccgc gaagcgcagc ttgcctcgtc gctggatcat
     1021 ccgaacatct gcactgtgca tgacttcata gaggctgacg gcgcgagctt catcatcatg
     1081 cagttcgtcg ccgggagcac agtccgccaa ctcgtcggag gtcgtcctct cgcaatcagc
     1141 acagctctga gaatcgcaat ccaggtctgc gacgcgctgg cggccgcaca ttccaaaggg
     1201 atcattcacc gcgacatcaa agcgcagaac atcatcgtct ccgaaaacgg acaggcgaag
     1261 attctcgact tcggcattgc gaaattgatc ggagaaggga gcgacggaaa ggatcagacg
     1321 gagatcaccg ccctcggctc gccgtatgga acgccgacct acgcggcgcc ggagcaatcg
     1381 cgaggggaga aagtcgatca ccgcgcggac atcttttcga cgggcgtatt gctttacgag
     1441 atgttttcag gcacctggcc gtttcagggg agaacggcaa tcgaggcccg ccacgccgtc
     1501 ctgtacgacg agccgcgccc gatcagtgaa aaccgaggcg gcccgatacc cgcgaagctg
     1561 gaagcgatcg tcagccgggc gctttcgaaa gagcccgcgc accgctatca acgaatggcg
     1621 gatatgcgcg atgccttgat cgaagtcttg cgggagctgc cggagtcgaa ggaagacagc
     1681 gcagcgggtt ttctggaagg cttccggccg tcaacgcttc gccattcgcc acgattgaat
     1741 tggaaattcg ggttgacggc ggccgccctt ctcgtcgtag tcgccgcgat actggcttac
     1801 ggcctctact cgagacaccg cgcgtctacc gcgatcggca agaaagacac aatcctcatc
     1861 accgatttca cgaatacgac cggcgacgcc gtctttgacg acacgctcaa acagggatta
     1921 gccatgcagc tgcagcagtc gccctttctg aacatcttcc ccggagcgcg cgttcgcgaa
     1981 acactgcgcc agatggagcg ctcgccggat gagcgggtga cggtggagac tggacgagag
     2041 atctgccagc gtaacgacct caaagctctg atcgccggtt cgattgccgc gctcggccgc
     2101 cattacgtca tcacgctcga agcaatcaac gccaagagcg gtgagacgat tggccgccaa
     2161 cagactgagg cggacagcaa ggaagaagtc ctgaagtcgc tctcatccgc cgccagtggc
     2221 atccgcgaga cactgggcga aagcctcagc tccatccgac aatcagacat tcctttgtat
     2281 caactcacga cgccgtcggt tgaagcgcta aagtcgttcg cactcggctt tgatctctcg
     2341 aacagggggg agtacttcaa atcaatcccg atgttcaagc gatccgtaga gctcgatgcg
     2401 aactttgctt atggctactc gctgctggcc ggcaattaca cgattgtaaa cgagccacgc
     2461 cgggccgcgg agaacgccgc gaaagcattt gctctcaaag acaagggaac ggatcgcgaa
     2521 cggctctaca ttacttccgt ttatgacaat tacacgctgg gtgacctgga caaggcaatc
     2581 gaagcttttc gtgtctacga tcagttctac gcaaacgatt tccgcgcttc gggcaatctg
     2641 tcgcttgcct atctgcttct gggacaatac gagaaggccc tcggagaggc gcgggagtcc
     2701 gtgcgcctca atccgggtat ttctgcctgg caggtgacgc tggggacgtc actcctccgc
     2761 ctcaaccgtt tccgagacgc caaagagacc ttcgatcgcg cgattgtgat gggtctcaat
     2821 gacgcacgga tgcacgacgc gctgtatcag cttgcgttcg ttcagcatga tgagaaaggg
     2881 atgcagcaac agctcgactg ggcgcgcggc cggccggagg agtacgtcgc cgccggcttg
     2941 caaagtgccg cagctgcgta tcgggggcaa tggagggagt cacagaccct tacgcagcgc
     3001 gccatcgacc tggcagtcca cgtcgacgtc aaagaagtgg ccgcgcgtta cgctgccgag
     3061 gagggtttgc gtgcggcggt gctgggaagg tgcgatcaag cgaaggtcta cgcggaacag
     3121 tcgctcgcac tcgatcataa tcaggtgaca caggagcgca tcgcgctgag ccaggctctt
     3181 tgcggcgacc gccgggcaca gtcatcgctg caaggggatg ttcaacaacg tccgggcgac
     3241 acactcctca acggcctctg ggtaccagtg gttcgtgccg catcggagct gaacgatggg
     3301 aacgcggaga aagcggtggc gtcgctggac aacacgcgga cctacgagcc cgccgcggaa
     3361 ttctggacgc actatattcg gggagaggct gagctgaagc tcaacagagg cacggacgcc
     3421 gcgtcagaat tccggcagat cctttcccat gccggagagg cgccgttgtc tattctctat
     3481 ccgcttgcgc aactcggact ggcacgggcc accgcgcaaa ccggcgctcg atcccagtcg
     3541 ctcaaggcgt accaggactt tctcaacagt tggaaagacg tcgatcccga tcttccaatt
     3601 gtccggtcgg cacgcgcgga atatgcgaaa ttgaaccgct aggtcctctg tgtctctgtg
     3661 gttaaaaacc tatgaaccac agagacacag agatcacaga gcagcgctac ctcccccaat
     3721 acaacgcatt gaaaaccatc ggataggtcg cgtgcgtctg gccgcggtgt tgcgggcgga
     3781 agccgaagag gacgatgcgg cctttgccga acgtcatcgc caccgcagcg gccttgcgtg
     3841 tcagtttttc ttcgccgttg atccagccgg agagaaggac gtcgcgggcg tcgtcgggat
     3901 acgttgccag aacccagcgc tgcatctcgt ttcccggcag cgtcgtctcg aacgcgatcg
     3961 gatgatcgat gaagactgct gtctcatccg gcatgcccgc cgtgaccgga tgatcggtgc
     4021 ggatgtggac gcgggccagc gagcctggaa cggagaaatc gttcggcgtc gctttcgcca
     4081 ggacgttgcg gacggggatg ttgaagttgt cgatgacgta gtcgcagccg gcgttgaagg
     4141 cgatgagggt gccgccgttc tcgacgaacg tgcgcaacgt ctgcgctcct tctttctcaa
     4201 gcgcgccgcg gttctcgggc ggcagctcgt cgatgtactt catcgatcct tcccctccgg
     4261 cgcggcgtcc ggtagcgacc tcctctttgt cgatgtcggg aatgatgaat acgtcgaagt
     4321 tctgcaggcc ggctttgact tcctgcgggt ggagcgactt cggcgtgaag ccgtaggaat
     4381 cgagcagcca gcgcgtccac ccctcgtcga gcgcgccgcc ccatgggttg tagatggcta
     4441 tgcgcggctt tggctgcgac gcgtgccgct tgatgacttc ctccgtctcc ggcttgatgc
     4501 tggtgacctg ttgaagcccg gttttcggag gcatgactgt cttctcgacc gtgacgccca
     4561 tcgcaagggg tagcgtccag gaggcgacgt cgtaagggcg gaggatgtct ttccccggtt
     4621 gcagacggac ctcgggatag cgttggggct cgagcagttc atccaggaat cgcgaatatg
     4681 gctgcgccat cgagatccag taatcgccgt tacccgcctg ctggatctcg acgccatgtt
     4741 cgaggaggag cgccgcgagg aactgtgcgg tcggccagtc gtgctgatcg tggggaatcc
     4801 ggtaggcgga cgtggcgccg aacgccaccg cagcgcgcgc acgcaccgct acatcgcgca
     4861 gaagatcttc gcggcggtcg gcggcggttt cgagtagtgc gtccgaggcg atgcgctcgt
     4921 agtccatgat gtcgcgcatc cgccacgtgc caccggccca tggatgcgga tggttgatcg
     4981 tcgctttgta gtcgatgagc cctttctgcc cgccgcgcag ctcgctcggg tcgatgacga
     5041 tgggcgatgc gatgcgcgct gacgccgtct cgagcagtag cccggtgatg ttcttccacc
     5101 agcctgtgtt gcgtgttccg ccgagccagt aggcatcgaa ggagaagccg tagatcagtc
     5161 ccgccttctc ctgctgctcg aggcgaaagg ccatgttcgt gccgacgagg ttcgcttcac
     5221 gccagatgag gggatggaca gtgggatcga gcggatcggc gaacggcggg atgaacatcc
     5281 gcggtccgtc ggtgcccatc tgatgctcgt cgacccaaac ctgcggatac cactcgtggt
     5341 acacggcgcg gctcagctcc ttcgtttcga gctgcgtgag catgaaccag tcgcggttgt
     5401 tgtcgtggcc gacgtagtga tggtagagcc agggaagccg gccgccctcg tagcgcgtgc
     5461 cgaggtactt gcggtagtag tcggtgatca tgatctggcc gtcagggttg agggacggca
     5521 cgagcaggag gacaacgttg tcgaggcggc gctttgtctc ggcgtcgttg gcgctggcca
     5581 gcgcgtgcac ccactccatc gccatctggc tcgaggcgat ctcggacgaa tggatgttgc
     5641 aggtcacgag gacgatcgat ttgccttcgc gggcgagttg cgagatctgt tcgtcgctga
     5701 gcccccgcgg gtccgccagt ttgcgcgcgg tctcccggat cttctccagg ttgcgcatgt
     5761 tcccctcaga cgtcacgaca gccatgatga agtcttcgcc gagggtggtc ttgccgagcg
     5821 attcgacctt gacgcgcggc gaggcagcgg ccagcgcgtg aaagtaggag acgatctgcc
     5881 ggtagtcggc aagcttgcgg tcggcgccga cttcgaaccc gagaaactgg gagggtgtct
     5941 ggatcgcggc ggtgagggcg gtggcggcga ggagaaggag gagggcgcag acgaagcgtt
     6001 tcatgcgcgg gggattatgg cggatcggag ggcatgaagg cttcgagagc tgttagaagg
     6061 cgcgggacct cgacggtggc tccaaaccaa acggcatcgt gaatgatggt cttgtagtcg
     6121 agagagactc tggtttgcag actcgcaacg tggggccaat cgatctccgg atgttgcgta
     6181 cgaactgcgt cgctcactcg ttccgcgttt ctgccgatcc gctgaagaag gtgagtcagg
     6241 gcgtattgaa gattctcatc agcgtcgaag tccgctcgtg acttattctc ggtgatcgac
     6301 atgaggcgat gagaggtgtc gagcattccg ccgagcggaa cgatgtcacg atgtctggtc
     6361 atactcctcg acagcttcgg tcaggacatc cttgatcatc caccggctga gcgctgccgg
     6421 cgtagacatg tcgacttttc gcccgaagat ttgagacagc tcctcctgcg ccccgaagaa
     6481 tgcaaaacct gtcgccgtcg tatccggccg caactccacc agcacatcca catcgctccg
     6541 ctccggatcg aagtcgtctc gcacgaccga cccg
//
LOCUS       DATLVW010000949         7285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_452157, whole genome shotgun sequence.
ACCESSION   DATLVW010000949 DATLVW010000000
VERSION     DATLVW010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452157"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..2408
                     /locus_tag="VNN25_28080"
     CDS             243..2408
                     /locus_tag="VNN25_28080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXH95468.1"
                     /translation="MTSDRGSSADGKTADESAASSSGELIDQRGGWWTTWGFAIAGSI
                     CLANVLFLQFVADRGTSSVLILKVSLLVAAAATFGASLWRAETRRRHLASLVRMAQES
                     SRRREAEVVAMREAKERAEAVSAAKSELFAGIAHELRTPLQGIIGMLRVAADSDASEK
                     RTQLLEMARRSAQSLLATIGDVLDFTKLEAGQPNLEPVYFSIRLLLADTLEPLGIAAA
                     EKSLTLAYAVAPEVPDRLWGDPGRVRQILVNLTGNAIKFTGSGEIVVRVGCEAIAADG
                     VTVVFEISDTGVGIDPSLRALLGNPLPSSSLRRQHGLGLPIVSRLLGAMGGAMTIESE
                     VGRGSTFRVLVPFSCEPIGTEPSPEWEKRLAGTRVLLIEPNATTRGILGEILRAHGMV
                     AEAYDSIERALQPSIRAAYGCLVADANLLAATPWIPPVPVVRIASPLVPPVGDGVFVT
                     RPCSERVLIQAVGAALGIAEPAIAYTLERRREILRPLRVLVVDDDGLNQELIADAARR
                     LGHVVTKTTAAEAPNLLAGREFDVVLVDVQAPQFDGLELARRFRAIERDGRTAAIAVT
                     TNATGEERDRCIAAGFDAVLQAPASQTAIGSILRDVTSPLTTARVPPQSAGSIFDAVG
                     GNANLLLRVRDAFATQIPRLLTAMRDAVEARDRDGLVQAARTLRASISNFDVPEPLAA
                     TLQIERAAAADDFARAAALLPAIETAIRELQEKIDAAVG"
     gene            complement(2535..6344)
                     /locus_tag="VNN25_28085"
     CDS             complement(2535..6344)
                     /locus_tag="VNN25_28085"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HXH95469.1"
                     /translation="MSSVTQTYGANTAFIEDLYESYRANPDSVSASWREFFHDYEPRF
                     EEEFEEEIEEQKAAAAGSTAFVAPISIPPAAPQASKPATQQPVTPQPLTPATPQPQPA
                     PRPTPVPSNQTTSPLRGAAGKIAQNMELSLSMPTATSIRNIPVKVLEENRRVVNNHLA
                     LTGQPKASFTHIIAWAIVNAVKQFPRMNAAFAMQADGTPSRIDHEDVNVGIAIDVERK
                     DGSRSLLVPNVKRGQTMDFAQFLKAYNEVVRKARNSTLEMSDFEGTTISLTNPGTIGT
                     IASVPRLMQSQGTIIATGQIDYPAEYSASDPSVLADLGISKVMTMTSTYDHRIIQGAE
                     SGAFLARVHELLLGADGFYEAIYKDLRIPYEPVVWGKDRHRLNASEDEKVAREAAVLQ
                     MINAYRVRGHLLADLDPLEYEVKRHPELDPAFYGLTIWDLDREFISGGLCGKLTAKLR
                     DILDTLRETYCGKIGPEYMHISETVQKKWLQDRMEPSRNHQPLELATKRRILMKLNDS
                     ESFEKFLHTKYVGHKRFSMEGAESTIPMLDYLFNEATQDGVAEAVIGMAHRGRLNVLA
                     NILGKSYEKIFHEFEGDVDPNTTQGSGDVKYHLGADGVHQTPDGSHMKLTLASNPSHL
                     EAVDPIVEGMVHAKQKLIGDHKRAWVLPVLLHGDAAFAGQGVVAETLNLSQLPGYRTG
                     GTIHLVINNQIGFTTGPESARSSVYATDVAKMVQAPIFHVNGDDPEACIRAVKLAYDF
                     RQQFHKDVVIDMICYRRHGHNEGDEPSLTQPKMYKSIKEHRSVRKIYTETLLRKGDID
                     PKEAESWLDNFQNKLQEAFDRTREDDAPPPSNERGALWTDEEITGFQNQPSPDTSVPR
                     NALDVVSKALTTVPADLTLHPKLKPIIARRTAMAEGREPIDWAFAEMLAFGTVMTDGF
                     RVRLSGQDSGRGTFSSRHAILFDYVTGKGLVPLNALATTGGASPATPASNATSSAPHV
                     IAENALGEQTASEGGAEVSFAVYDSLLSEYGVLGFEYGYSVADPGALVLWEAQFGDFV
                     NGSQIVIDQFISSAEEKWGQHSGLVLLLPHGYEGQGPEHSSARLERFLTLCAEGNMQV
                     IYPTTPAQHFHALRRQMKNDPRKPLIVMTPKSLLRHPQATSSIEELTSGRFEPVLIDP
                     ARDAAAVTRIVLSSGKVYYDLRTAREKANANLPLIRLEQFYPFPQAMLSAALAQYPSA
                     TEIVWVQDEPRNMGAWPFLHERLGALTSANQTLRYVGRPISASPATGSHHRHEEQQQA
                     LVAAAIG"
     gene            complement(6470..>7285)
                     /locus_tag="VNN25_28090"
     CDS             complement(6470..>7285)
                     /locus_tag="VNN25_28090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95470.1"
                     /translation="IASNVFGLDGKNVFRSVVMRIPFDALLRSMDRGDSLELSFLAES
                     DSGTFRCTLGARGTMYVVGQRDTQSLRIFAWPDEDEHPTARDVAVSEWIDGLEGYSAP
                     GPDGREWLRRADDRITAAWVAKGRVGVMWSVDRIDDRRPFPYVRVAILDEQTFRVIAE
                     PDIWSRDYAHVWPDACPNENGVVGITVFRGGRALYPSHTIGVLDDRTMTWTLVETIDG
                     TSGPSDKKWGDYLTCRAQSPDGSGWIAAGYTLQGGASVDRVEPFAVLFHVSDG"
BASE COUNT         1224 a         2208 c         2421 g         1432 t
ORIGIN      
        1 tccggtgatc ttgtcgccaa gcatgttctt ccggatctct tcctcaactg ctttgttgtc
       61 catccctgcg ggtgtgtttc ggttccagtt ggagcagccg atggacgaga ccagtgcggc
      121 ggaaagaacg agtgatgcga atgccttctt catactgata cctcctcgcg cgtgttgagc
      181 aatgagtgcg ccagaacgct ccggcgcgca ccggggcgcg agaacgcgta aagtctccgt
      241 acatgaccag tgatcgcggc agttccgctg acgggaaaac cgcggatgaa tccgctgcgt
      301 cttcttcagg cgagctgatc gatcagcggg gcggatggtg gacgacatgg ggattcgcga
      361 tcgccggttc gatctgtctg gcgaacgttc tgttcctgca gttcgtcgcc gatcgcggga
      421 cgtcgtctgt tctcattctg aaagtctctc tcctcgttgc cgcggcggcc accttcggcg
      481 cctcattgtg gcgtgccgag acacggcgcc ggcacctggc gagcctcgtg cggatggcgc
      541 aggagtcgtc gcggagacgt gaagccgagg tcgtcgcgat gcgggaggcg aaggagcgcg
      601 ccgaggcggt cagcgccgcg aagagcgagc tcttcgccgg catcgcccac gagctgcgca
      661 cgcctctgca aggcatcatc ggcatgctgc gggtggctgc cgacagcgat gcctcggaga
      721 agcgcacaca actgctcgaa atggcccgcc gttcggcgca gtcgctgctc gccacgatcg
      781 gagacgttct cgacttcacg aagctcgagg ccggccaacc taacctggag ccggtttact
      841 tttcgatccg gctgctgctc gccgacacgc tcgagccgct cggtattgcg gcggcggaga
      901 agagcctcac gctcgcctac gcggtcgcgc ccgaggttcc cgaccggctg tggggtgatc
      961 ccggacgcgt ccgacagatc ctcgtcaacc tgaccggtaa cgccatcaag ttcaccggca
     1021 gcggcgagat cgtcgttcgc gttggctgcg aagcgattgc ggccgacggc gtgacggtcg
     1081 tgttcgagat ctccgacacc ggcgtgggga tcgatccgtc gctgcgcgcg ctccttggca
     1141 atccattgcc gtcgtcgtcc ctgcgccgtc aacacggcct ggggttgccg atcgtgtcgc
     1201 gcttgctcgg cgcgatgggc ggcgcgatga cgatcgagag cgaagtggga cgaggaagca
     1261 cgtttcgcgt tctcgtcccg ttttcctgcg agccgattgg aacggagccg tcgccggaat
     1321 gggaaaagcg tctggcaggc acccgcgttt tgctgatcga gcccaacgcg acgacgcgcg
     1381 gcatcctcgg cgagattctg cgggcgcacg gcatggtggc cgaggcgtac gactcgatcg
     1441 agcgcgcgct gcagccgtcg atccgggctg cctacggctg tctggtcgcc gacgcgaacc
     1501 tgctcgcagc gacgccgtgg attccgcctg tgccggtcgt gcggatcgca tcgccactcg
     1561 ttccgccggt gggcgatggg gtcttcgtta cgcggccttg cagcgagcgc gtgttgatcc
     1621 aagccgtcgg cgctgccctc ggcatcgcgg agccggcgat cgcctacacg ctggaacggc
     1681 ggcgcgagat ccttcgcccc cttcgcgtat tggttgtcga tgacgacggc ttgaatcagg
     1741 agctcatcgc agacgcagcc cgtcgcctcg gccacgtcgt cacgaagacg accgctgccg
     1801 aagcgccgaa tcttctcgcc ggcagagagt tcgatgtcgt tctcgtcgac gttcaggcgc
     1861 cccaattcga cggactggaa ctggcgcggc gttttcgagc gatcgagcgc gatggccgaa
     1921 cggcggcgat cgcggtcacg acgaacgcga cgggggaaga gcgggaccgg tgcatcgctg
     1981 ccggtttcga tgccgtgctg caagcgccgg cgtcgcagac ggcgatcggg tcgatcctcc
     2041 gcgacgtgac gagcccgctg acgacggcgc gtgttccgcc gcaatccgcg ggctcgatct
     2101 tcgatgccgt cggcggcaac gcgaacctat tgctgcgcgt ccgcgatgcg ttcgccacgc
     2161 agatcccgcg cttgcttacc gcgatgcgcg atgctgtcga agcgcgagat cgcgacgggc
     2221 tcgtccaggc cgcacgcaca ctccgcgcgt cgatcagcaa tttcgatgtc cccgagccgc
     2281 tcgccgcaac gctacagatc gagcgcgcag cggccgcgga tgactttgcg cgcgcggccg
     2341 cactgcttcc cgcgatcgag accgcgatcc gcgagttgca ggagaagatt gacgccgcgg
     2401 tggggtgaat caatattagt gaatcaatat tagtcaataa gaagacacga accacagaga
     2461 cacagaggac acagaggtga agactttctc tgtgtcctct gtgtctctgt ggtgaaaagg
     2521 gtttgccagc gaaactaccc gatcgctgcg gcaaccagcg cctgctgctg ttcctcgtgg
     2581 cgatgatgtg aacctgtcgc cggcgatgcg ctgatcggac gtccgacgta gcgcagcgtt
     2641 tggttcgcgg aggtcagcgc gccgaggcgc tcgtggagga atggccaggc gcccatgttg
     2701 cgcggctcgt cctgcaccca gacgatctcc gtcgcgctcg gatactgcgc cagcgcggcg
     2761 ctgagcatcg cctgcggaaa cggatagaac tgttcgaggc ggatcaacgg caggttggcg
     2821 ttcgccttct cgcgtgcggt gcgaaggtcg tagtagacct tgccgctcga gagaacgatg
     2881 cgtgtgacgg ctgccgcgtc gcgcgcagga tcgatcagca ccggctcgaa gcggccgctc
     2941 gtcaattctt cgatcgacga tgttgcctgc ggatggcgca gcagcgactt cggcgtcatc
     3001 acgatcagcg gcttgcgcgg atcgttcttc atctgccgcc gcaatgcgtg gaaatgttgc
     3061 gcgggcgtcg tcggatagat cacctgcatg ttgccttccg cgcaaagcgt gaggaagcgc
     3121 tccaggcgcg ccgacgaatg ctccgggccc tgtccttcgt aaccgtgcgg aagaaggagc
     3181 acgaggccgc tgtgctgtcc ccacttctct tccgcggacg agatgaattg atcgatgacg
     3241 atctggctgc cgttcacgaa atcgccgaac tgcgcctccc acaatacgag cgcgccggga
     3301 tcggcgaccg agtagccgta ttcgaagccg agaactccgt actcgctgag tagtgagtca
     3361 tagacggcaa agctgacttc ggcgccgcct tcgctcgccg tctgctcgcc gagcgcgttc
     3421 tccgcgatga cgtgcggagc gctgctggtt gcgttcgacg ccggcgtggc aggcgatgcg
     3481 ccacccgtcg tggccagcgc gttgagcggc acgaggcctt tgcctgtgac gtagtcgaag
     3541 aggatcgcgt gccgcgacga gaacgtgccg cggccggagt cctgtccgga gaggcggacg
     3601 cggaagccgt cggtcatcac cgtgccgaat gcaagcatct ccgcaaatgc ccagtcgatc
     3661 ggttcgcgtc cctccgccat cgctgtccgc cgcgcgatga tcggtttgag tttcggatgc
     3721 agcgtcagat cagccgggac cgttgtcagc gctttgctga cgacgtcgag cgcgttgcgc
     3781 ggcacggacg tatccggcga aggttggttc tggaagccgg tgatctcctc gtcggtccag
     3841 agtgcgccgc gctcgttgga cggaggcggc gcgtcatctt cgcgcgtccg gtcgaacgct
     3901 tcctgcaatt tgttctggaa gttgtcgagc cacgactccg cttctttcgg atcgatgtcg
     3961 cctttgcgca gcagcgtctc ggtgtagatc ttccgcaccg agcgatgctc cttgatcgac
     4021 ttgtacatct tcggctgcgt gagcgacggc tcgtcgccct cgttgtggcc gtgacggcgg
     4081 tagcagatca tgtcgatgac gacgtctttg tgaaactgct ggcggaagtc gtacgccagt
     4141 ttcacggcgc ggatgcacgc ctccggatcg tcgccgttca cgtgaaagat cggcgcctgc
     4201 accatcttgg cgacgtcggt tgcgtatacc gaggagcgcg ctgattccgg accggttgtg
     4261 aagccgatct ggttgttgat cacgagatgg atcgtgccgc cggtgcgata gcccggaagt
     4321 tgcgagaggt tcagtgtctc ggcaacgacg ccctgtccgg cgaaggcggc atcgccgtga
     4381 agcagcaccg gcagcaccca ggcgcgtttg tgatcgccga tgagcttctg cttcgcgtgg
     4441 accatgccct cgacgatcgg atcgaccgcc tcgagatggc tcgggttcga cgccagcgtc
     4501 agcttcatgt gcgagccgtc cggcgtctgg tgcacgccgt cggcgccgag gtggtacttc
     4561 acgtcgccgg agccctgtgt ggtgttcgga tcgacgtcgc cttcgaactc gtggaagatc
     4621 ttctcgtacg acttgccgag gatgttggcg agcacgttga gtcgcccgcg atgggccatg
     4681 ccgatcaccg cctcggccac gccatcctgc gtcgcttcgt tgaagaggta gtcgagcatc
     4741 gggatggtgc tctcggcgcc ttccatcgag aagcgtttgt ggccgacgta cttcgtgtgc
     4801 agaaacttct cgaacgactc ggaatcgttg agcttcatca ggatgcgccg cttggtggcc
     4861 agctcgagcg gctggtgatt gcgcgacggc tccatccggt cctgcagcca tttcttctgc
     4921 acggtttcgg agatatgcat gtactccggc ccgatcttgc cgcagtaggt ttcgcggagc
     4981 gtgtcgagga tgtcgcgcag cttcgcggtc agcttgccgc acaagccgcc gctgatgaac
     5041 tcgcggtcga ggtcccagat ggtgaggccg tagaaggcgg gatcgagctc gggatggcgt
     5101 ttgacctcgt actcgagcgg gtcgagatcg gcgagcaggt gaccgcgcac gcgataggcg
     5161 ttgatcatct gcagcacggc cgcttcgcgc gccaccttct cgtcctcgct ggcgttcagt
     5221 cgatggcggt ccttgcccca gaccaccggc tcgtagggga tgcggagatc cttgtagatc
     5281 gcttcgtaga aaccgtcggc gccgaggagg agctcgtgga cgcgcgccag gaaggcgccc
     5341 gattccgcac cctgaatgat gcgatggtcg tacgtcgacg tcatcgtcat cactttggag
     5401 atgccgaggt cggcgagaac ggagggatcg ctggcggagt actcggccgg gtagtcgatc
     5461 tggccggtgg cgatgatcgt tccctgcgat tgcatcaggc gagggaccga cgcgatcgtg
     5521 ccgatcgtgc cggggttggt gagcgagatc gtcgtgcctt cgaagtccga catctccagc
     5581 gtgctgttgc gcgccttgcg cacgacttcg ttgtaggcct tgaggaactg cgcgaagtcc
     5641 atcgtctgtc cgcgcttgac gttgggaacg agcagtgacc gcgaaccatc tttgcgctcg
     5701 acgtcgatgg cgatgccgac gttgacgtct tcgtgatcga tgcgcgaagg cgtgccgtcc
     5761 gcttgcatcg cgaaagcagc gttcatgcgc ggaaactgtt tgaccgcgtt gacgatggcc
     5821 caggcgatga tgtgcgtgaa cgacgctttg ggctggccgg tgagggcgag gtgattgttg
     5881 acgacgcgcc ggttttcctc gagcaccttg acggggatgt tgcggatgga ggtcgccgtc
     5941 ggcatcgaca gcgagagctc catgttctga gcgatcttcc cggccgcgcc gcggagcggg
     6001 ctggtggtct ggttcgaggg gacgggagtg gggcggggag cgggttgcgg ttgcggggta
     6061 gcgggggtga gaggctgcgg tgtgacgggt tgctgcgttg ccggcttgct ggcttgcgga
     6121 gcggcaggtg ggatcgagat cggagcgaca aacgcagtcg agcccgcggc tgccgccttc
     6181 tgctcctcga tctcctcttc gaactcctcc tcgaagcgag gctcgtaatc gtggaagaac
     6241 tcgcgccagc tagcgctcac cgaatcagga ttggcgcggt agctctcgta aagatcttcg
     6301 atgaatgccg tgttggcgcc gtaagtctgg gtgacagatg acatagcggg cttcctaacc
     6361 gtgattgggc tgctctgatt tcgatgacag acggatcagg gaggcgtcgt acctcgtccg
     6421 ggctgcgtcg caactgggat gcaggaaatg agcgttgatg ctgcgagatt tatccatcgg
     6481 agacatgaaa gagcaccgcg aatggctcca cgcggtcgac cgaagcacca ccttgcagcg
     6541 tatatccggc tgcaatccag ccgcttccat ccggagattg cgcgcggcaa gtgaggtagt
     6601 caccccattt cttgtcagaa ggaccgcttg ttccatcgat cgtctcgacc agcgtccaag
     6661 tcatcgtacg gtcgtcgaga acgccgatgg tatgcgaggg gtagagcgca cggccaccgc
     6721 gaaaaaccgt gataccgacg acaccgtttt cgttgggaca agcatcgggc catacgtgcg
     6781 cgtagtctct gctccagatg tccggctcgg caatcactcg gaatgtttgc tcgtcgagaa
     6841 tggccacgcg gacatagggg aacgggcggc ggtcgtcgat ccgatcaacg gaccacatga
     6901 ctccaacacg acctttcgca acccatgccg ctgtgatgcg atcgtcggca cgcctcagcc
     6961 attcgcggcc atccggaccg ggcgccgaat acccttccag tccgtcaatc cactcactca
     7021 ccgcgacatc gcgtgctgtc ggatgctcgt cttcgtcggg ccaggcaaag atccgcagcg
     7081 actgggtatc acgctgaccg acgacgtaca tcgtgccgcg ggctccgagc gtgcagcgga
     7141 aggtaccact gtcgctttca gcaagaaaac taagctccaa cgaatctcca cgatccatcg
     7201 atcgcagcag cgcgtcgaac ggaatgcgca tcacgaccga gcgaaagacg ttcttgccgt
     7261 cgagtccgaa cacgtttgag gcgat
//
LOCUS       DATLVW010000950        13075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_494311, whole genome shotgun sequence.
ACCESSION   DATLVW010000950 DATLVW010000000
VERSION     DATLVW010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494311"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..748)
                     /locus_tag="VNN25_28095"
     CDS             complement(41..748)
                     /locus_tag="VNN25_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXH95471.1"
                     /translation="MSVRPRAKFQRLTPMLTGVAEEIEVSYNPTELTFSKSAQIAEIP
                     IPGLDSPILQFVRGQTETLSVELFFDSTDDGMGDDATAVTEKTDRFYQLIKIDRSTHA
                     PPVCRFIWGDKGFAGSKMTGTWASQNRQNGFQCVVESVKQRFTLFNAKGRPLRATLTL
                     SLKEYRTLERQIAEIFQQSPDHTHAHVVQRGDTLARIAAERYANPAQWRAIADENRID
                     DPLELVPGTILEVPPLR"
     gene            complement(745..930)
                     /locus_tag="VNN25_28100"
     CDS             complement(745..930)
                     /locus_tag="VNN25_28100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95472.1"
                     /translation="MDVHVGEMNSTVRVGDSGGLSAETFQRIVDAVLARAREELARDA
                     RVKDELRLRTSASEQQP"
     gene            complement(941..1468)
                     /locus_tag="VNN25_28105"
     CDS             complement(941..1468)
                     /locus_tag="VNN25_28105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HXH95473.1"
                     /translation="MAIGLFGQRLDPVRTYNFVITLADSASVLGTILSGIQNTLLGGF
                     SECSGLEMDLKTEDFNEGGNNGTVLKFPTRVTWANIHLKRGAALNDELWKWHYSFVEG
                     TGTRRDGIIMLQDDEHQPVKMWTFKRGLPVKWTGPTFNAMQSQLAIEELEIAHEGLSI
                     QNPGSIIGGIVKSLF"
     gene            complement(1468..3780)
                     /locus_tag="VNN25_28110"
     CDS             complement(1468..3780)
                     /locus_tag="VNN25_28110"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HXH95474.1"
                     /translation="MYTAPGVFFEAQDEAAGQVRRLRTDIAGFTGVCERGPLDTPVRV
                     TSWEQFRTAFGNLISNALLPWAVKAFFENGGQRCWIVRVAAPEATSSSAGVQPADRMS
                     SIVPAGTTNGFAAGAAVTLMQSSVTFTSALAQPADRLSSLIVDPAGFTQGSLVRIVQA
                     AKPSTYAHLIAVDAVAKQFFWDRALDPAYDITLPIELHTTQQRNRLLLDADATTLFWS
                     RPVDDVFDLLRPIDFATGASAASAMLLDQHSNPTLRVDAASTGAWGNELQVSVARTSL
                     HATRTTGAQPASGLSSNVESVTGFGPGTVVRLFQNGAGITHRVVTHVDYARRSLVWST
                     AIVLPFDVTKPIAFESVEFTITVSESGRIREAFEDLSLERTDRNRYAPDVVNARSRFI
                     RIDDQLSPSPVPESNPDPQSPALDRGRLVLRGGRDGIAALRTIEVTGSMATDERKGLR
                     ALELIDEVAILAIPDVLIERVPPVESAPPPQPPEPDPCFPAPPAPPVAIPPPPKIREQ
                     SATFSEDEIVLVQSAMIDQCERLLDRFTLLDPPNFRSTDFGEIESWRRRFETKYAALY
                     FPWIVVVDPLSRGASLTRALPPSGHVAGIYARSDLAIGVHKAPANEELQWAQDVTLEV
                     TQNLHEVLNPIGVNAIRSIAARGLRVNGARTVSSDPAWRYVNVRRLISMIEEALDESL
                     QWAVFEPNNDQLRVKVATAIAAFLEQLWLSGMLAGKTLGEAFYVDAHVDEERLLAEVG
                     VAPAIPAEYVVVRVGRTEDVLEISEPEGAA"
     gene            complement(3802..4239)
                     /locus_tag="VNN25_28115"
     CDS             complement(3802..4239)
                     /locus_tag="VNN25_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HXH95475.1"
                     /translation="MATGKRIDPYHGYNFEVQIDNTSVGGFRECGGLSFTTDPVEYRE
                     GTDVPLHVRKLHGLRKFANITLKRGITKSADLWTWYKNVLNGKDDRRNGAIILHDEQH
                     NPVVRWNFENGWITKWEGPAVNATSNDVALESVEIAVERVELV"
     gene            complement(4252..6330)
                     /locus_tag="VNN25_28120"
     CDS             complement(4252..6330)
                     /locus_tag="VNN25_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HXH95476.1"
                     /translation="MAEYLAPGVYVEEVDSGIKTIEGVSTSIAGFAGVTARGPVKGLP
                     KLVTSFSEFERNFGGYFDFGAAFAGHNYLPYAVNGFFANGGRQLYISRVLKGDATVAE
                     GTAKGGLITRLRADALVGGTTFNPVTQRGMKIAVVGPPAQVATKVRLVMVKNGIVYQS
                     SDRTVTVVNPATGVVTVNPALDITPTGPATFEAKYTYVMTDIKDLNGGGVINALGAVG
                     GTRNDAIVLKANDKGSWGRDIVVTAAHETPVRAEMDAGGDIGGTGAADSTRIGLKSAK
                     GFYKGAWVEVERNDYKYFRLVDSVDGNIIRLHGPSIAAADLGGSVFVSVCEFRLTVSY
                     GGVTETFSGLTLEDVIGKNFKRRINGESALLDNVDLAGGALNDPTYFPSGDNGLNLVL
                     GTNGTNGTAAPGINDYIGTGDPGDRSGIKALEDIDRISIIAVPGRTDLAVQNALIEQC
                     ERLKDRFAIADPANSKVVDILTQRGQFDTKYAAIYFPRILVFDPLTENDLAVPPSGHM
                     AGIYARVDIERGVHKAPANEVVRSITGYEATINKETQDTLNPSPTNVNVFRDFRADGR
                     GLRIWGARVMTSDTEWKYINVRRLFIFLEESIDEGTQWVVFEPNDERLWARVTQSISA
                     FLTRVWRDGALLGTTAEEAFFVKCDRTTMTQDDLDNGRLICIVGVAPVKPAEFVIIRI
                     SQFTASAATE"
     gene            complement(6353..7363)
                     /locus_tag="VNN25_28125"
     CDS             complement(6353..7363)
                     /locus_tag="VNN25_28125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95477.1"
                     /translation="MTTIVTDTGTFRVVPDLPLDRVAVFVARLVDEVSGAEPRSLVGI
                     QTNREAAYAREASGGMLAVSGRVEHLFPELATKAYSLDLTIDGPGYRPVKRTDNISIG
                     SLFPIDLGTIPLRPYPIRVEGRVTQDSNRNPIAGASVSVITPKLALLRVPAYRDHPAG
                     TNVTVQTLTPGSTRSLGAGAPAGTLTFLLSSVASLAANGVLLFGDSEYGIIDSIDSPA
                     KTVTLKHALQRSYAINATVKPVTTAAGAATTTARDVNAGDGILILSATLTGNAVDIGG
                     AEYHDLGVLSDAGGFYAANGVGGVRELTFRASAGGFIDLDVPLTIDYASPNNPLTFKL
                     RT"
     gene            complement(7360..7953)
                     /locus_tag="VNN25_28130"
     CDS             complement(7360..7953)
                     /locus_tag="VNN25_28130"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HXH95478.1"
                     /translation="MSEFTAMRAVSMTLQSMLDAEITQSSDPQLKGVPIHLFSPKVVH
                     LKEKTGISLWLYRVQREPDTLNRPQERITPTQLRHRPMPLMLHYLVCAVFDEPKDEQA
                     LLGKVVQVLNDHAIIRGSALKDSMQGEDLELRLVLEALTLEELARVWDSLNEPYQLSL
                     SYVVQVVEIDSDRDIVQRAPVLVGTTLANQILERTGS"
     gene            complement(7950..8225)
                     /locus_tag="VNN25_28135"
     CDS             complement(7950..8225)
                     /locus_tag="VNN25_28135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95479.1"
                     /translation="MSIPSEQAAVRDVVRYFVEHPESADSLEGIVRWRLLERRFRETS
                     DETAAAVGWLVGHGFLQQIDIPGGRQLYRLPAERLSQAAQLLGENVS"
     gene            complement(8382..9719)
                     /locus_tag="VNN25_28140"
     CDS             complement(8382..9719)
                     /locus_tag="VNN25_28140"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXH95480.1"
                     /translation="MSCLLVIGTTAEVSSFRQAAPEPATQCAFVDWQQFRPEMLRERT
                     ERLVVIFASGADGELCRWLRLHPIPQAILAIFTGELHVELLETVDDFLLWPATSAELQ
                     CRIERLMHGLASDSGEVCRKLLDELAVAQLVGSEPCFVETIRQLALFARSEMPILITG
                     ETGTGKELCARAAHFLSARRNGPFVAVDCSAIPDHLFENEMFGHARGAYTDARAEQKG
                     LATLSEHGTLFLDEVDSLSLAAQRKLLRFLQEKTFKPLGGEKFLHADVTVLAATNRDL
                     AQTVATGAFRSDLYYRLNVLQVCVPPLRERRGDVNLLARHFLESACARMQHRKTFSPQ
                     TLHKLTMYDWPGNVRELMNLVQRAAIVAEGDVVLPCHVPLPGFDVAEEEATFRSARAK
                     TLEIFERAYVRDMLAKHDGNVSRAARAAGKERRAFGRLVKKYNLARGSVTTRT"
     gene            10163..11977
                     /locus_tag="VNN25_28145"
     CDS             10163..11977
                     /locus_tag="VNN25_28145"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HXH95481.1"
                     /translation="MHIRILIALSLFAACGSVRAADPPQPAPASSRSPAGEGYVAVQP
                     APRFHRGVVKGPFGPPRKVSYQVINGHARFEGDIDLGPVDAQGNLISKPINTAVGVID
                     IAQVWPDCVVPFRFDASVSSDMMALMTSAMTTWSSATPCTFPAHTTEKDYVTFQTYKD
                     TKSNGGGHSDIGHIGGEQGVHINEKETNPFVIVHEIGHTLGLFHEHTRHDRDQFVTIV
                     WKNIDSTYSHDFEIDKDSQDIGVYNFTSVMHYPSTAFGSPKGATTILPNVSGVTIGQR
                     SFLDPGDISGAMEVQGIYRYWTPFETMQSPPAAASPGVIGPFDSAYYFVPFFSGGRDV
                     GQLCRRYFPWYPPEDCDTGAPPEPLRGIPAATVSPVAKGETAIFVRGLDTNSYLDYRR
                     DYLTNKAGGAFHLKTARAGTNWLSDPASITLNLQRIIVFGLRGRVDHPGETVVALVYK
                     DSADGSWTGPIELPPVSTGPMFEPAVISTRPGIWTLFVFDTTGTLWVTEGNESGTVVT
                     PWKPVTKFSVASAGPKSGPSVTVTGVPNDQILLTINGRSNHLFYLGMDNSFTVTDDWH
                     DIGGLLNSRPSVAAIGPGAFVLANIQGGIPLWWRWWKP"
     gene            12462..>13075
                     /locus_tag="VNN25_28150"
     CDS             12462..>13075
                     /locus_tag="VNN25_28150"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXH95482.1"
                     /translation="MSVDGSSNEERPGTGRRYRTDRTEAISDGVFAVSLTLLVLDVRP
                     PEGGVSQLVHGLVLIAPRLGTFALSFAIVAYYWLVHHLIFASLRGAKVALMWANMLFL
                     FTIAVLPFSTAVLGRYSLAPVALAIYGANLFACTSTLAGVWLVADHSHITEQPTASQR
                     RYIVRRFGVQLIVALLGIACAFLAPALALSIFVALPIVFALTYHR"
BASE COUNT         2209 a         4207 c         4179 g         2480 t
ORIGIN      
        1 cctctttgta aatcggtacg ggcgacatgt cgttcaggcc tcagcgaagg ggcgggacct
       61 ccaggatcgt tccagggacg agctcgagcg gatcgtcgat gcggttctcg tcggcgatcg
      121 ctctccattg cgcgggattg gcgtagcgct ccgcggcgat gcgggcgagc gtgtcgccgc
      181 gctgcacgac gtgcgcgtgg gtgtggtcgg gcgactgctg aaagatctcc gcgatctgcc
      241 gctccagcgt gcggtattcc ttcaggctca gcgtcagcgt cgcgcgcagc ggccgcccct
      301 tcgcattgaa gagcgtgaag cgctgcttga cgctctccac cacgcactgg aatccgttct
      361 gccggttctg gctcgcccag gttccggtca tcttcgagcc ggcgaatccc ttatcgcccc
      421 agatgaaacg gcagaccgga ggggcgtgcg tggagcggtc gatcttgatc agttgataga
      481 agcgatcggt cttctccgtc accgccgtgg cgtcgtctcc catcccgtcg tcggtgctgt
      541 cgaagaaaag ctcgacggag agcgtctccg tctgaccgcg gacgaattgc aggatcgggc
      601 tgtcgagccc ggggattggg atctccgcga tctgcgcgct cttgctgaac gtcagctcgg
      661 tcgggttgta cgagacctcg atctcctcgg cgacgccagt gagcatcggc gtgaggcgct
      721 ggaatttcgc gcgcggacgg acgctcatgg ctgctgctcc gaggcgctcg tgcgcagccg
      781 caactcgtcc ttcacccggg cgtcgcgggc cagctcttcg cgggcgcgcg ccagcacggc
      841 atcgacaatc cgctggaagg tctcggcact gaggccgccg ctgtctccca cccgcacggt
      901 gctgttcatt tcgcctacgt gaacgtccat cgcatcgctc ctagaaaaga ctcttcacga
      961 ttccgccgat gatcgagccg gggttctgga tgctcagccc ttcgtgggcg atctccagtt
     1021 cttcgatcgc cagttgactc tgcatggcgt tgaaggtggg accggtccac ttgaccggca
     1081 gcccgcgctt gaaggtccac atcttgacgg gttgatgctc gtcgtcctgg agcatgatga
     1141 ttccgtcccg ccgcgtcccc gttccttcga cgaagctgta gtgccacttc caaagctcgt
     1201 cgttcagcgc cgcgccccgc ttcaggtgga tgtttgccca cgtaacgcgc gtggggaact
     1261 tgaggacggt gccgttattg cccccttcgt tgaagtcctc cgtcttgagg tccatctcca
     1321 gcccgctgca ctcgctgaat ccgccgagga gcgtgttctg gatgccgctg aggatcgtgc
     1381 ccaggacgct tgcactgtcg gcgagcgtga tcacgaagtt gtacgtgcga accggatcga
     1441 ggcgttgacc gaatagtccg atggccatca cgccgctcct tcgggttcgc tgatctcgag
     1501 cacgtcctcg gtgcgcccga cgcgcacgac gacgtattcc gccgggatcg caggggccac
     1561 gccgacttcg gcgagcaggc gctcctcgtc gacgtgcgcg tcgacgtaga acgcctcgcc
     1621 gagcgtcttg ccggcgagca tgccggagag ccagagctgt tcgaggaacg cggcgatcgc
     1681 cgtcgccacc ttcacgcgca gttgatcgtt gttcggctcg aacaccgccc actgcagcga
     1741 ttcgtcgagc gcttcctcga tcatcgagat gagtcgccgg acgttgacgt accgccaggc
     1801 cggatcgctg ctcaccgtgc gcgcgccgtt gacgcgcagg ccgcgcgcgg cgatgctgcg
     1861 gatggcgttg acaccgatcg gattaagaac ttcgtgcagg ttctgcgtca cttccagcgt
     1921 gacgtcctgc gcccactgca gctcttcgtt cgccggcgcc ttgtgcacgc cgatggcgag
     1981 atcgctgcgg gcgtagatgc cggcgacatg tccgctcggc ggaagcgcgc gtgtgagcga
     2041 cgcgcctcgg ctcagcggat cgacgacgac gatccagggg aaatagagcg cggcgtactt
     2101 cgtctcgaat cgccggcgcc agctttcgat ctcgccgaag tcggtggagc ggaagttcgg
     2161 cggatcgagg agcgtgaagc ggtccagcaa ccgctcgcac tgatcgatca tcgcgctctg
     2221 caccagaacg atctcgtcct ccgaaaacgt ggcgctctgc tcgcggatct tcggcggcgg
     2281 cggaatcgct acaggcggcg cgggaggtgc ggggaagcat ggatcgggct ccggcggctg
     2341 tgggggcggc gcgctctcga ccggtggcac gcgctcgatg aggacgtcgg gaatggccag
     2401 gatggccacc tcatcgatca gctcgagcgc gcgcagacct ttccgctcgt cggtagccat
     2461 cgagccggtg acctcgatcg tgcgcaacgc ggcgatgccg tcgcgtcctc cgcgcagcac
     2521 gagccgcccg cgatcgaggg ctggcgactg cggatcggga ttcgattccg gaacgggcga
     2581 cggcgacagt tgatcgtcga tgcggatgaa gcgcgagcgg gcgttcacga cgtcgggcgc
     2641 gtaacggttc ctgtcggtgc gctcgagact cagatcttcg aacgcctcgc gaatgcgccc
     2701 gctctcggaa accgtgatcg tgaactcgac gctttcgaat gcgatcggct ttgtgacgtc
     2761 gaacggcagc acgatcgccg tgctccagac gagacttcgc cgcgcgtagt cgacgtgtgt
     2821 caccacgcga tgcgtgatcc cggcgccgtt ctggaaaagg cggacgaccg tgccggggcc
     2881 gaagccggtg acgctttcga cgttcgatga gagtccgctc gccggctgcg cgccggtggt
     2941 gcgcgtggcg tgcagactcg tgcgggccac gctcacctgc agctcgttgc cccacgcgcc
     3001 ggtactggcg gcatcgaccc gtagcgtcgg attggagtgc tgatcgagca gcattgccga
     3061 cgccgccgag gcgccggtgg cgaagtcgat cggccggaga aggtcgaaca catcatcgac
     3121 cggccgcgac cagaagagcg tcgtagcatc ggcgtcgagc aggagcctgt tgcgctgctg
     3181 cgtcgtgtgc agctcgatcg gcaaggtgat gtcgtacgcg gggtccagcg ctcgatccca
     3241 gaagaactgt ttcgccaccg cgtcgacggc gatcagatgc gcgtaagtgg acggcttcgc
     3301 cgcctgcacg atgcgcacga gcgagccttg cgtgaagccg gccggatcga cgatcagcga
     3361 tgagaggcgg tctgctggtt gcgcgagcgc gctggtgaac gtgacgctcg actgcatcag
     3421 tgtcaccgcg gcaccggcgg cgaatccgtt tgtcgttccc gccggaacga tggaggacat
     3481 gcggtccgcc ggctgcaccc cggcgctgga gctcgtcgcc tccggcgcgg cgacgcgtac
     3541 gatccagcag cgctgcccgc cgttttcgaa gaacgccttc accgcccacg gcagcagggc
     3601 gttggagatg aggttgccga aggccgtgcg gaactgttcc cacgacgtaa cgcgtaccgg
     3661 cgtgtcgagc gggccgcgct cgcacacgcc ggtgaatccg gcgatgtcgg tgcgcaggcg
     3721 gcgcacctgt ccggcggctt cgtcctgagc ctcgaaaaag acacccggcg ctgtgtacat
     3781 cggcacggca aggccctccg attacacgag ctcgacgcgc tcgacggcga tctcgacgct
     3841 ctcgagcgcg acgtcgttgc tggtggcgtt cacggccgga ccttcccact tggtgatcca
     3901 gccgttctcg aagttccagc gcacgaccgg gttgtgctgc tcgtcgtgca ggatgatggc
     3961 gccattgcgc cggtcgtcct tgccgttgag aacgttcttg taccaggtcc agaggtcggc
     4021 gcttttcgtg atgccgcgct tgagcgtgat gttcgcaaat ttgcgcaggc cgtggagctt
     4081 gcgcacgtgc agcggcacgt ccgtcccttc gcggtattcg accggatcgg tggtgaagct
     4141 caggcctccg cactcgcgga agccgccgac cgaggtgttg tcgatctgca cctcgaagtt
     4201 gtagccgtgg taagggtcga tgcgtttgcc tgtggccatg gcattcctcc gttactcggt
     4261 cgccgcactg gcggtgaact ggctgatgcg gatgatcacg aactcggctg gtttaaccgg
     4321 cgcgacgccg acgatgcaga tcagccttcc gttgtcgagg tcgtcctgcg tcatcgtggt
     4381 ccggtcgcac ttcacgaaga acgcctcctc ggcggtggtg cccagcaatg cgccgtcgcg
     4441 ccagacgcgc gtgaggaacg cggagatgct ctgcgtcacg cgcgcccaga ggcgctcgtc
     4501 gttcggctcg aagaccaccc actgcgtccc ttcgtcgatc gactcctcga ggaagatgaa
     4561 gagccggcgg acgttgatgt acttccattc cgtgtcgctg gtcatcaccc gcgcgcccca
     4621 tatccggagc ccgcggccgt cggctcggaa gtcgcggaag acgttgacat tcgtcggact
     4681 gggattgagc gtgtcctgcg tctctttgtt gatcgtcgct tcgtagccgg tgatgctccg
     4741 cacgacctcg ttggccggtg ccttgtgcac gccacgctcg atgtcgacgc gggcgtagat
     4801 gccggccatg tgtccgctcg gcggaacggc cagatcgttc tcggtcagcg gatcgaacac
     4861 gaggatgcgc gggaagtaga tggccgcgta cttggtgtcg aactgaccac gctgcgtgag
     4921 gatgtcgacc actttcgagt tcgccggatc ggcgatggcg aagcggtcct tgagccgttc
     4981 gcactgttcg atgagcgcgt tctgcaccgc gagatcggtg cggccgggga cggcgatgat
     5041 gctgatgcgg tcgatgtcct cgagcgcttt gattccactg cggtcgcccg gatcgccggt
     5101 gccgatgtag tcgttgatgc ccggcgcggc ggtgccgttg gtgccgttcg tgccgaggac
     5161 gagattcagg ccgttgtcgc cagacgggaa ataggttgga tcgttcagcg cgccgccggc
     5221 cagatcgacg ttgtcgagca gcgccgattc gccgttgatc cgccgcttga agttcttgcc
     5281 gatgacatct tccagcgtca gtccgctgaa agtctccgtc acgccgccgt acgagacggt
     5341 gaggcggaac tcgcaaaccg agacgaagac ggagccgccg agatcggccg ccgcaatcga
     5401 cggcccgtgc aggcggatga tgttgccgtc gaccgagtcg acgaggcgga agtacttgta
     5461 gtcgttgcgc tccacctcta cccatgctcc cttgtagaac cccttcgccg acttcagtcc
     5521 gattcgcgtc gagtcggcgg cgccggtgcc accgatgtcg ccgccagcgt ccatctccgc
     5581 gcgcaccggc gtctcgtgcg cagcggtgac gacgatgtcc cgtccccacg accctttgtc
     5641 attcgccttg agcacgatgg cgtcgttgcg cgtaccgccg accgccccca gcgcgttgat
     5701 gacgccgccg ccgttgaggt ccttgatgtc ggtcatgaca taggtgtatt tcgcctcgaa
     5761 cgtggcgggc ccggtaggcg tgatgtcgag cgccggattg accgtgacga ctccggtggc
     5821 ggggttgacg acggtgacgg tgcggtcaga tgactggtag acaatcccgt tcttcaccat
     5881 caccagccgc actttcgtcg ccacctgcgc tggtggcccg acgacggcga tcttcatgcc
     5941 ccgctgcgtg acgggattga acgtcgtccc gcccaccagc gcatcggcgc gcaggcgcgt
     6001 gatcaggccg cccttggccg tcccttcggc gacagtggca tcgcctttga gcacgcgcga
     6061 gatgtagagc tgccgcccgc cgttggcgaa gaatccgttc accgcatacg gcagatagtt
     6121 gtggccggcg aacgctgctc cgaaatcgaa gtagccgccg aaattccgtt cgaactcgct
     6181 gaagctcgtc accagcttcg gcagtccctt caccggcccg cgcgcggtca ccccggcgaa
     6241 accggcgatg ctcgtgctca ccccctcgat ggtcttgatg ccactgtcga cctcttcgac
     6301 gtagacgcca ggcgctagat attcagccac gacttcatct cctatgtttc ggtcaggtcc
     6361 tcaacttgaa ggtcagaggg ttgttcggac tcgcgtagtc gatcgtcagc ggcacatcga
     6421 gatcgatgaa gccgccggcg gatgcacgga aagtgagctc gcgtacgcct ccgacgccgt
     6481 tcgcagcgta gaagccgccg gcgtcggaga gcacgcccag gtcgtgatac tcggcgccgc
     6541 cgatatcgac ggcgttgccg gtgagcgtcg cgctcaggat caggatgccg tcgccggcgt
     6601 tcacgtcgcg cgctgtggtc gtggccgcgc cggctgcggt ggtcaccggc ttcacggtcg
     6661 cgttgatggc gtagcttcgc tgcagggcgt gcttcagcgt gacggtcttc gctggagaat
     6721 cgatgctgtc gatgatgccg tactcgctgt caccgaagag cagtacgcca ttcgcggcta
     6781 acgacgccac gctgctcaga aggaaggtca gcgtgccagc gggtgccccg gcgccgagcg
     6841 agcgcgttga tcccggcgtg agcgtctgca cggtcacgtt cgtgcctgcg ggatggtcgc
     6901 gatacgccgg aacgcgcagc agggcgagct tcggtgtgat gacgctgacg gaagcacccg
     6961 cgatcggatt gcgattgctg tcctgcgtga cccggccctc gactcggatc ggatagggcc
     7021 gcagtgggat cgtgcctaga tcgatcggaa acaacgagcc gatggagata ttgtctgttc
     7081 gcttcaccgg ccggtagccg ggaccatcga tcgtcaggtc gagcgagtag gctttcgtcg
     7141 ccagctcggg gaagaggtgc tcgacgcgcc cggacaccgc cagcatcccg ccgcttgctt
     7201 cgcgggcata cgccgcctca cgattcgtct ggatcccgac cagcgaacgc ggctcggcgc
     7261 cgctcacctc gtcgacgagt ctggccacga acaccgccac gcggtccaac ggcaggtcgg
     7321 ggacaacccg gaacgtcccg gtgtcggtca cgatcgtcgt catgaccccg tcctctcgag
     7381 aatctgattc gccagcgtcg tcccgacgag tacgggcgct cgctgcacga tgtcgcggtc
     7441 ggagtcgatc tcgaccacct gcacgacgta cgaaagcgag agttgatacg gctcgttcag
     7501 cgagtcccag actcgcgcga gctcttccag cgtgagtgcc tccagtacga gccgcagctc
     7561 gaggtcttca ccctgcatcg agtccttgag cgcgctgccg cgaatgatcg cgtgatcgtt
     7621 gagcacctgc acgaccttgc cgaggagggc ctgctcgtct ttcggttcgt cgaagaccgc
     7681 gcagacgagg taatgcagca tgagcggcat cggacgatgg cggagctgtg tgggagtgat
     7741 gcgttcctgt ggccggttca gcgtatcggg ctcgcgctgc acgcgataca gccaaagcga
     7801 gatgccggtc ttctctttca ggtgcacgac cttcggcgag aagagatgga tcggcacgcc
     7861 cttgagctgc gggtcgctgc tctgcgtgat ctcggcgtcg agcatcgact gcagcgtcat
     7921 gctgacggcg cgcatggcgg tgaattcgct cacgacacgt tctccccgag cagttgcgcg
     7981 gcctgactca aacgttcggc aggaagtcga tagagctgac gcccgcccgg tatgtcgatc
     8041 tgctgcagaa aaccgtgtcc gacgagccag ccgaccgcgg ctgcggtctc gtcgctagtc
     8101 tcgcgaaagc gccgctcgag cagccgccaa cgaacgatcc cttcgagcga gtcggccgac
     8161 tcgggatgct cgacgaaata gcgcacgacg tcgcggacgg ccgcctgctc gctcggaatc
     8221 gacatgaggt gccgatccgg ttcgcaagcg ggacgccagg cttaagtcat tggaaacact
     8281 agaagtgtgg aagcggtgtg ggttgcgcgc gacccgcgtg gacgctccgg aaaatctcca
     8341 gcgagcggag acactctcat ctgagccggg tcatctccac cctacgtccg ggtcgttact
     8401 gacccacggg cgaggttgta cttcttcacc agccgtccga aagcgcggcg ctctttccct
     8461 gccgcacgcg cggcgcgcga gacgttgccg tcgtgcttcg ccagcatgtc gcggacgtac
     8521 gcgcgctcga agatctccag cgtcttcgcg cgtgccgacc ggaacgtcgc ctcttcctcg
     8581 gccacgtcga aaccgggcag cggtacgtga cacggcagca cgacatcccc ctcggccacg
     8641 atcgccgcgc gctgcacgag attcatcagc tcgcggacat tacccggcca gtcgtacatc
     8701 gtcagcttgt gcagcgtctg cggcgaaaac gtcttccgat gctgcatacg cgcgcatgcg
     8761 ctctcgagga aatggcgcgc cagcaggttg acgtcgccgc gccgctcgcg cagcggcggc
     8821 acacacacct gcaggacgtt gagccggtag tagaggtcac tgcggaacgc gcccgtggcc
     8881 acggtttgcg ccagatcgcg attcgtcgcg gccagcaccg tcacgtccgc atgaagaaac
     8941 ttttcgccgc cgagcggctt gaaagtcttc tcctgcagga agcgcaggag cttccgctgc
     9001 gccgcgagcg aaagggaatc gacctcgtcc aggaagagcg tgccgtgctc ggaaagcgtg
     9061 gccagtccct tctgttcggc acgcgcatcg gtgtacgcac cgcgcgcgtg gccgaacatc
     9121 tcattctcga agaggtgatc gggaatcgcg ctgcagtcga ccgcgacgaa cggtccgttg
     9181 cgccgggcgc tgaggaaatg cgcggcgcgc gcgcagagct ctttcccggt tccggtctcg
     9241 ccggtgatca ggatcggcat ctcgctcctg gcgaagagcg cgagctggcg gatggtctcg
     9301 acgaagcacg gctcgctacc gacgagctgc gccaccgcta gctcgtcaag gagcttgcgg
     9361 cagacctcgc cggagtcgga cgccaggccg tgcatcaatc gctcgatgcg gcattgcaac
     9421 tccgcggacg tcgcaggcca gagcaggaag tcatccacgg tctcgaggag ctcgacgtgc
     9481 aactcgccgg tgaagatcgc gaggatcgcc tgcggaatcg gatgcaagcg cagccatcga
     9541 cagagctcgc catcggctcc ggacgcaaag atgacgacga ggcgttccgt gcgctctcgc
     9601 aacatctcag gccggaattg ctgccagtcg acgaacgcgc attgcgtggc cggctcaggg
     9661 gcagcctgtc gaaaagacga cacttccgcc gtcgtaccga tgacaagcag gcatgacatc
     9721 gttgaacctg ggccttcgtt ctctcgagtc gcctatgagt tccgttgttc aggtagtaat
     9781 agcacacccg cgtacttccg tcatgaggag aactgaagaa atctcttccc cccgggtgac
     9841 cggttgaacc ggatcgttgc atctgtcgcg aacgaaagga acgtcgtcga tatccgatcg
     9901 accggccacc tcccccggat agtcgcggtt ttcttatcca gggccataat ggtattcaga
     9961 cggacagggt tcgagaaatc tggcaggcag ggggagccgg atggcttgtg caattggtcg
    10021 tgggtcaatg cgtcccggtg catggatcac gagctcatgc tcccccaagg ggacgtccgt
    10081 cttgagagcg gtgaactgac cgtcggtgag cgaacctcgc ggaccgaggt agggaaactg
    10141 ccgagctccg cctctcctcg gtatgcacat tcgcattttg atcgctctat ctctgttcgc
    10201 cgcgtgcggc tccgttcgag ccgccgatcc cccgcaaccg gcacccgcct catcgcgttc
    10261 gccggccggc gagggctacg tcgccgtgca gccggcgccg cgattccata gaggtgtcgt
    10321 caaaggacca ttcggcccac ctcgaaaggt cagctatcag gtcatcaacg gccacgcgcg
    10381 gttcgaaggc gacatcgacc tcgggccggt cgacgcgcaa gggaatctga tctcgaagcc
    10441 gatcaacacc gcggtcggcg tcatcgacat cgcccaagtc tggccggact gcgtcgttcc
    10501 cttccgcttc gacgccagcg tcagttccga catgatggcc ctcatgacca gcgccatgac
    10561 cacctggtcg agcgccacgc cgtgcacctt ccccgcgcac accaccgaga aggactacgt
    10621 cacatttcag acctacaaag atacaaagag caacggcggc ggtcactctg atatcgggca
    10681 cattggcggc gagcagggcg tccacatcaa cgagaaggaa accaatccct tcgtcatcgt
    10741 gcacgagatc gggcacacgc tcggcctttt ccacgagcac acgcgccatg accgcgacca
    10801 gttcgtcacc atcgtctgga agaacatcga ttccacctac agccacgact tcgagatcga
    10861 caaagactca caggacatcg gcgtttacaa cttcacctcg gtgatgcact acccatcgac
    10921 agcattcggc agtccgaaag gcgcaaccac gatcttgccc aatgtgtcgg gcgtgacgat
    10981 cgggcagcgc tcgttcctcg atccgggcga catcagcggc gcgatggaag tccagggcat
    11041 ctatcgctac tggacgccgt ttgagacgat gcagtcgccg ccggccgcgg cctctcccgg
    11101 ggtcatcggt ccgttcgatt cggcctacta cttcgtgccc ttcttcagcg gcgggcgcga
    11161 cgtcggacaa ctctgccgcc gctacttccc atggtatcca cccgaggact gcgacaccgg
    11221 cgcaccgccc gagccgctcc ggggcatccc ggcagcgacc gtgagtccgg tggcgaaagg
    11281 cgagactgcc atcttcgtcc gcggcttgga caccaatagc tatctcgact accgccgcga
    11341 ctatctcacc aacaaggcgg gcggcgcgtt tcacttgaag acggcgcgtg cgggaaccaa
    11401 ctggctgtcg gatccagcat cgatcacgct caaccttcag cgcatcatcg tcttcggtct
    11461 ccgcggccgc gtcgatcatc cgggtgagac ggtcgtggcg ctggtctaca aagacagcgc
    11521 cgacggatcg tggaccgggc cgatcgaact gccgccggtc agcaccggtc cgatgttcga
    11581 gccggccgtc atctccacgc ggcccggcat ctggacgctg ttcgtcttcg acacgacggg
    11641 aacgctgtgg gtgacggagg gcaacgagag cggcacggtc gtgacgccgt ggaagcccgt
    11701 gacgaaattc agcgtcgcca gcgcggggcc gaagagcggt ccttccgtca cagtcactgg
    11761 agtgccgaac gaccagatcc tgctaacgat caatggccgt tccaatcatc tcttctacct
    11821 cggtatggac aacagcttca ccgtgacgga cgactggcac gacattggcg gcctactcaa
    11881 cagccgcccg tccgtggccg ccatcgggcc cggcgcgttc gtactggcca acatccaggg
    11941 cggcattccg ctctggtggc gttggtggaa gccttagcgc gaatcgaggt atgttccaag
    12001 acagccgcgg aattggaaat caggagttcg ccgccgtctt catagacgcg ttcctgatct
    12061 tcacgtaacg ccgcctgagg gtcaggtcta aagtctaaag tgccgaattg gcctgatcgc
    12121 attagcgcca atcgtcgtcg gcactttaga ctttagacct gaccctcaac ccaccctcct
    12181 gacgttcgcg ctccgtaaca gaatctattg acgcggtggc acttttcggc cataaaatcc
    12241 aagtgctcga ctcgtaacgc ggtcgtgtga ggggccgggg aggtgagcgc caatcacaat
    12301 ctcctccggc tcctcggttt aacctctgcg gcggggttga atcagacggc ttgggcggcg
    12361 tcggcgaaga ttctgccggc gctgccttct tgcgtttcgt cggtttcccc aacgcgcgct
    12421 tcgtcggatc ccgctcaaaa ggcaattgtc gggagaattt catgagcgtc gacggttcgt
    12481 cgaacgagga aagaccagga accggtcgga gatatcgaac cgaccggaca gaagcgatta
    12541 gcgacggagt cttcgcggtt tcgctgacgc tcctcgtcct cgacgtacga cctcctgaag
    12601 gcggcgtgtc ccaactcgtg cacgggctcg tcctgattgc accgcggctc gggaccttcg
    12661 cgctgagctt cgcaatcgtt gcgtactact ggctcgtcca ccacctgatc ttcgcctcac
    12721 tgcgcggcgc aaaggtcgct ctgatgtggg cgaacatgct gtttctattc acgattgccg
    12781 tattgccctt ctccaccgcg gtgctcggtc gttactctct cgcgccggtg gcgctcgcaa
    12841 tttatggcgc caatctcttc gcttgtacgt cgacgctggc aggtgtgtgg ctcgtcgcgg
    12901 accattctca catcacagaa caacccacgg ccagccaacg acgctacatt gtgcggcgtt
    12961 tcggtgttca gttgattgtc gcgctgctgg ggatcgcatg cgcgttcctc gctccggcac
    13021 tcgcactgag catcttcgtc gcgctgccga ttgtgttcgc gctgacctac caccg
//
LOCUS       DATLVW010000951         4572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_411402, whole genome shotgun sequence.
ACCESSION   DATLVW010000951 DATLVW010000000
VERSION     DATLVW010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411402"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VNN25_28155"
     CDS             complement(<1..317)
                     /locus_tag="VNN25_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HXH95483.1"
                     /translation="MLNDYHFITRWRVRGTVQEVLDILGDAEDLPRWWPSVYIEVKKQ
                     GDIVELFTKGWLPYTLRWSFRVTEKRPDGFDIEAFGDFAGRGEWLLTQDGDDVGIIYD
                     WRIT"
     gene            complement(385..3777)
                     /locus_tag="VNN25_28160"
     CDS             complement(385..3777)
                     /locus_tag="VNN25_28160"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXH95484.1"
                     /translation="MNHERLERRDWFLIAACLAIAAVSIFVVLNWFSAAFPEASIEFR
                     YDRDSSQQLAARVVAAQQIDTRGMKHSATFDSDEEARIFLERSVGLKKANEIFKRDVR
                     LWSWSHRWFRPLQEEEIRVEIAPTGEVTGFSDRIPEDRAIASPDVAGARRIAEGFLSS
                     VHANLGDLQLVSQSERRLPHRMQRIFTWESRTVHPAGAPYRTVVTVDGNRIRSFSTRV
                     KVPEDWQRSYRELRSKNLLAGNVDTVFFIITGICIVAVFVTRLIRGDLRLRLLLGIGT
                     AAIVLVTGTTLNSWPSALAGYDTTNSYPAFLAQFAFGAVLQGVGVAMLLVVIVGAGEV
                     MFRGRFPEKLSFARIWTPRALTSKRVFLSFIIGYTLVAFFMCYQTAFYLIADKFGAWA
                     PAEVPYDDMLNTAFPWIAVLFAGFFPALSEEFMSRAFSIPFFEKVLRSRVAAIVAAGF
                     IWGFGHATYPNQPFYIRGVEVGLAGVLIGFLMMRFGILPLLIWHYTVDALYTAMLLLR
                     SGNRYYVVSGGLASLVFAIPMLISIALYIRNKGFVPDDDLSNETLPLKPVPERPEVTV
                     GPAFTFPAMPVTRGKLLACIVIILLAAGALALHLPSIDDAVDYRITKEDAKRIASRIG
                     PPPTAAMRTIATPVEGFRSWERDSPREDGGTPGGYDGLAVDYILRNGMKVPALVSVMK
                     TKIPTATWMVRSFAPQVKDEYFIEVDPRTSRVTGYHKYQDERRAGPRLEQPQALAIAQ
                     GAFAQYNEDPRAFDLKEALSYQQQNRRDWLFHFQEKQPLAADAWRRISVRVAGADVTE
                     FTTTVKVPDSVYREPTTLLNIVFTITRIVGGLAILSLIVAGFVIALRRQRFPWRRALR
                     WTLALAIIPLIAFAARWESTLFGYNTSIRWETFISGQVIDVVRNVGLQLGLVFLAVAA
                     LAAAYPQAFHLARRDARAALGRSAVVAALTAIGIALLFRIGEQALALRFPSLAAMQSF
                     NVPDAVALPLPALLGIFSAILRTLIFCAVFGLFLLALRGFRAKPWLPPTIGIAALFFA
                     TLDSSANLKQTPLMLVTAAATAILAFVVIRYVFGANLLAYPLTIAVALLVGNGADLLQ
                     NHRPDLRVNGIIEIVVAVGLLLWAALPNPLHHRDTESTEIPL"
     gene            complement(3785..3949)
                     /locus_tag="VNN25_28165"
     CDS             complement(3785..3949)
                     /locus_tag="VNN25_28165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95485.1"
                     /translation="MIGSLGCPELIIIGAIGLVFPAGIFALGFFIGRRSARRDTNVTP
                     TDATSGPPHV"
     gene            complement(3949..>4572)
                     /locus_tag="VNN25_28170"
     CDS             complement(3949..>4572)
                     /locus_tag="VNN25_28170"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXH95486.1"
                     /translation="EGGDNHFRPEYFRAVRQLCDENDMLLILDEVQSGVGLTGKMWAF
                     QHYGIEPDMFCFGKKTQVCGFAAGNRLFDIDDNVFAVSSRINSTWGGNLTDMVRSQRY
                     FEVIDEEGLVENAAVVGAYFKSELQRFADEFPDMVSNVRGRGLMTAFDLPSAELRDAA
                     LKSFLANDVMALSSGSRSARFRPPLNLSQDEAAEGIKRMEKALQGLV"
BASE COUNT          919 a         1377 c         1524 g          752 t
ORIGIN      
        1 gtgatgcgcc agtcgtagat gatgccgacg tcgtcgccgt cttgcgtcag cagccactcc
       61 ccgcggccgg cgaagtcgcc gaaggcttcg atgtcgaatc cgtcggggcg tttttcggtg
      121 acgcggaagc tccagcgcaa cgtgtacggg agccagcctt tggtgaagag ctcgacgatg
      181 tcgccttgct tcttcacttc gatgtagaca gagggccacc agcgcggaag atcttcggcg
      241 tcgccgagga tgtcgaggac ctcctggacc gttccgcgga cgcgccagcg ggtgatgaag
      301 tggtagtcgt tgagcatgtt cttctctacg acggaatgtt gattgcggat tcgctaaaag
      361 ctttgcacca cagagacaca gaggtcacag aggtatctct gtgctctctg tgtctctgtg
      421 gtgcaaaggg tttggtaaag cagcccagag aagcagcccc acagcaacga cgatctcgat
      481 gatgccattg acacgaagat cggggcgatg gttctgcagc agatccgcgc cgttgccgac
      541 gagtagcgct accgcgatcg tcagcgggta ggcgagcagg ttcgcgccga atacgtagcg
      601 gatcacgacg aaggcgagga tcgcagtcgc cgcggcggtc acgagcatca aaggtgtctg
      661 cttcaggttc gcggacgagt cgagagtggc gaagaagagc gccgcgattc cgatcgttgg
      721 cgggagccac ggcttcgccc ggaacccgcg cagcgccagc aggaacaggc cgaagaccgc
      781 gcagaagatg agcgtgcgca agatcgcgga gaagatgccg aggagggcgg ggagtggcag
      841 ggcgacagcg tcggggacgt tgaatgattg catcgctgcc agcgacggga agcgcagcgc
      901 gagcgcctgc tcgccgatcc ggaagagaag ggcgattccg attgccgtca gcgcggcaac
      961 gacggctgag cggccaagcg cggcacgagc atcgcggcga gcgagatgga atgcctgcgg
     1021 atacgcggcc gcgagcgcgg cgacggcgag gaacactaat cccaactgca gaccgacatt
     1081 gcgcacgacg tcgatcacct gtccgctgat gaacgtctcc cagcgaatcg acgtgttgta
     1141 tccgaacagc gtcgactccc agcgcgcggc gaaggcgatc agcggaatga tcgccagcgc
     1201 cagcgtccaa cgcaacgcgc gtcgccacgg gaagcgctgc cgccgcaacg cgatcacgaa
     1261 gccggccacg atcagcgaca gaatcgccaa tcctccgacg atgcgcgtga tggtgaaaac
     1321 gatattgagc agcgtggtcg gctcgcggta gacactgtcc ggcaccttca ccgtggtcgt
     1381 gaattcggtg acgtccgcgc ccgccacgcg cacgctgatg cgccgccagg cgtcggcggc
     1441 gagcggctgt ttttcctgga agtggaagag ccaatcgcgg cggttctgct gctgatagct
     1501 cagcgcttct ttcagatcga acgcacgagg gtcttcgttg tactgcgcaa acgcgccttg
     1561 cgcgatcgcc agcgcctgcg gctgctcgag gcgcggtccg gcgcggcgct catcctgata
     1621 cttgtgatag ccggtcacgc gcgacgtacg cggatcgact tcgatgaagt actcatcctt
     1681 cacctgcggc gcgaacgagc gcaccatcca cgtcgccgtc gggatcttcg tcttcatcac
     1741 gctgacgagc gcagggacct tcatcccgtt gcgcaggatg tagtcgacgg ccagcccgtc
     1801 gtagccgccc ggcgtgccgc cgtcctcgcg cggcgagtcg cgctcccatg aacggaagcc
     1861 ttccaccggc gtcgcaatcg tgcgcatggc cgcggtgggc ggagggccga tgcgcgaggc
     1921 gatgcgtttc gcgtcttctt tcgtgatgcg gtaatcgacc gcgtcatcga tcgacggaag
     1981 atgcagcgca agagcgccag cggccagcaa gatgatgacg atgcaggcca gcagcttgcc
     2041 gcgcgtcacc ggcatggccg ggaacgtgaa cgctggcccg accgtcacct cggggcgctc
     2101 aggaacaggt ttcagcggaa gcgtttcatt cgagagatcg tcgtccggga cgaagccttt
     2161 gttgcgaatg tacaaagcga tcgagatcag catcgggatc gcaaagacga ggctggccag
     2221 accgccggag acgacgtagt aacgattgcc ggagcgcagc aacagcatcg ccgtgtacag
     2281 cgcgtcgacc gtgtaatgcc agatcagcag cggcaggatc ccgaaacgca tcatcaggaa
     2341 tccgatcagg acgccagcaa gcccgacttc gacgccgcgg atgtagaacg gctgattcgg
     2401 atacgtggcg tggccgaatc cccagatgaa tccggcggcg acgatcgcgg ccactcgcga
     2461 ccgcagaacc ttctcgaaga acggaatcga gaacgcgcgc gacatgaact cttcgctgag
     2521 cgcggggaag aatccggcga agagcaccgc gatccacggg aacgccgtgt tgagcatgtc
     2581 gtcgtacggc acctcggccg gcgcccacgc gccaaacttg tcggcgatca aatagaacgc
     2641 cgtctggtag cacatgaaga atgcgaccag cgtgtagccg atgatgaacg agaggaagac
     2701 gcgcttcgac gtgagagcgc gcggcgtcca gatgcgcgcg aaggagagtt tttccgggaa
     2761 gcggccgcgg aacatcacct cgcccgcgcc gacgatgacg acgagcaaca tcgccacgcc
     2821 gacgccctgc aggacggcgc cgaacgcgaa ttgcgccagg aacgcgggat aggaattggt
     2881 cgtgtcgtag ccggccagcg cactcggcca agagttcagc gtcgttccgg tgacgagaac
     2941 gatggccgct gttccgatgc cgaggaggag acggaggcgc aggtcgcctc gtatgaggcg
     3001 cgtgacgaac accgccacga tgcagatgcc ggtgatgatg aagaagacgg tgtcgacatt
     3061 tccggccagc aggttcttcg agcgcagctc ccgatacgag cgctgccaat cctcggggac
     3121 cttgacccgg gtagagaaac tgcgaatgcg gttgccgtcg acggtgacga cggttcgata
     3181 cggcgcgccg gcaggatgga cagtgcgcga ttcccacgtg aagatgcgct gcatccggtg
     3241 gggcagccgc cgctcgcttt gggagacgag ctgaagatcg ccgaggttgg cgtgaacgct
     3301 ggagaggaac ccctcggcga tgcggcgcgc gcccgcgacg tcgggagacg cgatggcgcg
     3361 atcctcggga atccgatcgc tgaagccggt cacttcgccg gtcggcgcga tctcgacgcg
     3421 gatctcttcc tcctgcaacg gccggaacca gcgatgcgac cacgaccaga gacgcacgtc
     3481 gcgtttgaag atctcgttcg ctttcttcag gccgaccgag cgttccagaa agatgcgcgc
     3541 ctcctcgtcg gagtcgaacg tcgcgctgtg cttcatccca cgcgtgtcga tctgttgtgc
     3601 agcgacgacg cgcgccgcga gctgctgcga tgagtcgcgg tcgtatcgaa actcgatcga
     3661 cgcctcgggg aacgcagcac tgaaccaatt aaggacgacg aagatcgaga cggcagcaat
     3721 ggcgaggcag gcggcgatga gaaaccagtc gcgccgttcg agtcgctcgt gattcattcg
     3781 ttgactatac gtgcggcggc ccggacgttg cgtcggtcgg tgtgacgttt gtgtcccgtc
     3841 gcgccgaacg gcggccgatg aagaatccaa gcgcgaagat accggccggg aagacgagtc
     3901 cgatcgcgcc gatgatgatc aattcggggc aacccagcga gccaatcatc agaccagccc
     3961 ctgtagcgct ttttccatcc gcttgatgcc ctccgccgct tcgtcctgcg agagattcag
     4021 aggcggacgg aatcgcgcgg agcgactgcc ggaggagagc gccatcacgt cgttggcgag
     4081 gaatgacttc agcgcggcgt cgcggagctc ggcactgggg aggtcgaacg cagtcatcag
     4141 cccgcgcccg cgaacgttgg aaaccatgtc cgggaactcg tcggcgaagc gctgcaattc
     4201 gctcttgaag tacgcgccga ccactgcggc attctcgacg agcccttctt cgtcgatgac
     4261 ctcgaagtag cgctgcgagc ggaccatgtc ggtgaggttg ccgccccacg tggagttgat
     4321 gcgcgagctg acggcgaaga cgttgtcgtc gatgtcgaaa agacggttgc ccgcggcgaa
     4381 tccgcagacc tgcgtcttct tgccgaagca gaacatgtcc ggctcgatgc cgtaatgctg
     4441 gaacgcccac atcttgccgg tcaggccgac gcccgactgc acctcgtcga gaatgaggag
     4501 catgtcgttc tcatcgcaga gctggcgcac ggcgcggaag tactcgggac gaaaatggtt
     4561 gtcgccccct tc
//
LOCUS       DATLVW010000952         4840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_577644, whole genome shotgun sequence.
ACCESSION   DATLVW010000952 DATLVW010000000
VERSION     DATLVW010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577644"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..900
                     /locus_tag="VNN25_28175"
     CDS             112..900
                     /locus_tag="VNN25_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXH95487.1"
                     /translation="MDVDQPAGHEIHIPPKPREALEDRKLLDRPSRQELLQSESWRVL
                     RITSEFVAGFDHLAEVYPAVSIFGSARTQLANEYYEAAVKTAELLGKAGFAVITGGGP
                     GIMEAANKGARQAGALSIGCNIELPFEQRVNQYVDKAMTFRYFFVRKTMFIKYSEAFI
                     TFPGGFGTLDELMGALTLIQTKKITNFPVILYGSEYWGGLLDWLRKSVLGANNITKED
                     VDSLHLVDDPVKVRDIVCAFHDRAVVTNGDEDESRPPAHVRRED"
     gene            1458..1652
                     /locus_tag="VNN25_28180"
     CDS             1458..1652
                     /locus_tag="VNN25_28180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95488.1"
                     /translation="MRYRYFLRAAITSTPRFTHVDDHFAYTVAIQGAAGENQQMYPAV
                     RFGDCDDNLPDPATAPPAKL"
     gene            1667..3073
                     /locus_tag="VNN25_28185"
     CDS             1667..3073
                     /locus_tag="VNN25_28185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95489.1"
                     /translation="MRKLRTIFLAFMFTSLLDVSAQAQSSISGTGGSVTTTGTDVTVT
                     TGSTNSTSGFHIFNSSATELLRVQSNGNVGIGTSPARRLHVVDTGSDIQARFGDGSRD
                     IAIEGATGAGTIELFRLQNVLGSKMLLKGGAVAIDASGYVGIGTTTPAYRLHSNGLIG
                     ITNLGTAVVGGGMRFDKDATPTVSWSIGGSDKDGTLTSDLYIAQFLSGAWTDRVTLQS
                     GGNVGIGTASPARRLNVVDTGADIQAKFGDGSRDIAIEGATGAGTIEMFRLQNVAGSK
                     MLLKGGAVAIDGSGNVGIGTAGTPITAKLDVRGSLVVSGGDLAVSGNITGGTVRATFQ
                     DVAEWVPATGDVPQGTVVVLDPTTSNAVVPSSHAYDTTVAGVVSARPGLLLGEAGTSK
                     AKVATTGRVKVRVDATTHAIHIGDLLVTSDQPGTAMFSEPLDLAGVRLHRPGTLIGKA
                     LEPLASGQGEILVLLSLQ"
     gene            complement(3108..4436)
                     /locus_tag="VNN25_28190"
     CDS             complement(3108..4436)
                     /locus_tag="VNN25_28190"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXH95490.1"
                     /translation="MPTWYLLLALLFIIANGFFVAAEFSIIKVRPTQLAEMAAAGSAR
                     ARMTRRIVKKLDSYLSATQLGVTLASLALGWVGEPAFAQLVQPRLSFFGVYSNSVAHT
                     IAASVAFIVISSLHIIFGELGPKYLAIDKTLGTALWSAHLLRAFYLVMFPIIWALNRA
                     SNGVLRLIGVKPASGAEMVHSQDELRLILAHSEKAGILSEENREIIEGVFQFSKRTAR
                     QIMVPRTDVSVLSTLKSIEENLEIIRITRHTRYPLCDGTLDQTIGLIHVKDLFLAQLR
                     GPGRTMLELKRDVLFVPENSTVEALLSQFIEHKTHMAIVLDEYGGASGIVSLENITEE
                     LFGQIQDEFDRERPEIEPLGNGRYRVRGDYLIEDLADRLKVDVGEPAEETIGGYVAAR
                     IGHEVVPGDRTQLGDLAIEVLDAERFRVRWVIVQTEPPATEEAEESEEQS"
     gene            complement(4523..>4840)
                     /locus_tag="VNN25_28195"
     CDS             complement(4523..>4840)
                     /locus_tag="VNN25_28195"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXH95491.1"
                     /translation="LASSPVEIVNVANLRVKESDRLATVTSELRRLGVTVEERPDSLL
                     IQPGWTSEPVTVATYNDHRIAMAFAIAGLARGNVTIADEQVVSKSYPRFWKTLDEVTA
                     PPS"
BASE COUNT          956 a         1436 c         1511 g          937 t
ORIGIN      
        1 atggggggtt gggagtgggg agtgggagaa tagctctcag cagttttttt ctctcgatcc
       61 tcgatctacc actccccact ccccactccc cgaacccgca taatgtcttc gatggacgtt
      121 gatcaaccgg ccggacacga aatccatatc cctcccaaac cgcgggaagc gctcgaggac
      181 cgcaagctcc tcgaccgccc cagccggcag gagttgctgc agagtgagtc gtggcgcgtg
      241 ctgcgcatca cgagcgagtt cgtcgctggc ttcgatcatc ttgccgaggt gtatccggcg
      301 gtgtcgatct tcggatcggc gcgcacacag ctcgcgaacg agtactacga agcggcggtc
      361 aaaaccgctg agctcctcgg caaggctggc ttcgcggtca tcaccggagg cgggcccggc
      421 atcatggagg ccgccaacaa gggggcgcgg caggcgggtg cgctctcgat cggatgcaac
      481 atcgagctgc cgttcgagca gcgcgtcaac caatacgtcg acaaggcgat gacgttccgc
      541 tacttcttcg tgcgcaagac gatgttcatc aaatactcgg aggcgttcat cacgtttccg
      601 ggcggcttcg gaacactcga cgagctgatg ggcgcgctga ccttgatcca gaccaagaag
      661 atcacgaact tcccggtgat cctctacggg agcgagtact ggggcggtct gctcgactgg
      721 cttcgcaagt ccgtcctcgg cgcaaacaac atcacgaagg aagacgtgga cagcctccac
      781 ctcgtcgacg atccggtcaa ggtccgcgac atcgtctgcg cattccacga ccgcgcagtg
      841 gtcacgaatg gcgacgagga tgagtcgcgg cctccagccc acgtgagacg cgaggactga
      901 acgccgtttc gtgggctccc tctttgcagg tcatttgacg catgggggaa acgccgatgc
      961 gaattcagga gacggacaag atcctcaaag aagcgcgcta cgcccgacac ggccacgagc
     1021 ttcatcagcg cagccgcggc ggcctctggc agcgagcgtg gcgatcggtg acggccctct
     1081 tcaaacattc gtcaaccaat cggcgggagg ttacgactgt tccccgctct cttccgcctc
     1141 ttcggtcgcc ggcatctccg tctgcacgat gacccagcca gtcggagcat gaagcgcacg
     1201 cggagcatga aaggtcgtcc agcctgacga aagcgttctc aacgtaccgg tcaattgctt
     1261 ctggctgaaa tattactgcc tgaatgcgcg ccgcctaacc ccgataaaaa actcgtgggt
     1321 gtggatttct cttcccgcga cactcctacc agtgaaggcc cgattcgggt cctcggcgat
     1381 tcaagggcga tcagtgtgag gcctgcaggc gcaagcgtgg ccggcggtcg tctgtccagc
     1441 gaaagggagg ttgttctatg cggtaccgtt actttcttcg ggctgcaatt acgtcgacac
     1501 cgcgcttcac gcacgtcgac gatcacttcg cttacactgt cgccatccag ggcgcagcgg
     1561 gcgagaacca gcagatgtac ccggctgtgc ggttcggtga ttgcgacgac aatctccctg
     1621 atccagctac cgcgccgccc gcgaagctgt aaggcatgag acgatcatgc gaaaacttcg
     1681 aacgatattc ctggcattca tgtttacctc actgctcgac gtatccgcgc aggcacaaag
     1741 tagtatcagt ggcaccggcg gctcggtgac cacaactggc accgatgtca ccgttaccac
     1801 cggcagcacc aattcgacct ctggatttca catattcaac tcgagtgcga cagagctact
     1861 acgagtgcag tccaacggca atgtcggtat tggaacgagc cccgctaggc gtctacacgt
     1921 cgtcgatacg ggcagtgata ttcaggccag atttggtgat ggaagcaggg acattgccat
     1981 agaaggagcg acaggcgccg gaaccataga actttttcgt ctgcaaaacg tcctcggttc
     2041 aaagatgctc ctgaagggcg gggccgttgc gattgatgct tctggctacg tcgggattgg
     2101 gacgacgacg ccagcttaca gattgcattc taatgggctg ataggaatca ccaatttagg
     2161 caccgccgtt gtgggtggcg ggatgagatt tgataaggat gccactccaa cagtttcctg
     2221 gtcaatcggc ggtagtgata aagacgggac actcaccagt gatttgtaca ttgcccaatt
     2281 cctttccggt gcgtggaccg acagggtcac gctgcaatcc ggcggtaacg tcggtattgg
     2341 aacggcgagc ccagcccggc gtctaaacgt cgttgatacg ggcgctgata ttcaggctaa
     2401 atttggtgat ggaagcagag atattgccat agaaggagcc acaggcgctg gaaccataga
     2461 gatgtttcgt ctccagaacg tcgccggttc aaaaatgctc ctgaagggtg gggccgttgc
     2521 tattgatggt tctggcaacg tcggtattgg tactgcggga acgccgatca ctgcgaagct
     2581 tgacgtgcgc ggcagcctcg tggtcagcgg cggcgacctc gcggtcagcg gcaacattac
     2641 cggcggcaca gtaagggcta cgtttcagga tgtggcggag tgggtgcctg ctactggcga
     2701 cgtgccgcag ggcacggtcg tcgttcttga tccaacaacg agcaatgcag tcgtgccctc
     2761 atcgcatgcc tacgacacca cggtcgcagg ggtcgtctcg gcccggcctg ggcttctgct
     2821 cggtgaggca ggtacttcaa aggcgaaggt ggccaccacc gggcgcgtca aagtccgagt
     2881 cgacgctaca acgcacgcga ttcacatcgg cgatctcctt gtcacgagcg accagccggg
     2941 caccgccatg ttctccgagc cactcgattt ggccggcgtg aggctccacc gacccggcac
     3001 cctcataggc aaggcccttg agccgctcgc gagcgggcag ggcgagattc tcgtgttact
     3061 cagcctccag taagcggcct gtccttctcg cgcagctcat tgggtactta agactgctct
     3121 tcgctctctt ccgcctcttc ggtcgccggc ggctccgtct gcacgatgac ccagcgcacg
     3181 cggaagcgtt ccgcatcgag cacttcgatt gccagatcgc ctagctgcgt gcggtcgccg
     3241 gggacgactt cgtggccgat gcgtgctgcg acgtagccgc cgatcgtctc ctctgccggc
     3301 tcgccgacgt cgactttcaa tcgatcggca aggtcctcga tgaggtaatc gccgcgaacg
     3361 cggtatctgc cgttgccgag cggctcgatc tccggccgct cccggtcaaa ctcgtcctga
     3421 atctgcccga acaactcctc ggtgatgttt tcgagcgaca cgatgcccga cgcgccgccg
     3481 tactcgtcga gcacgatggc catgtgcgtc ttgtgctcga tgaactgcga gagcagcgct
     3541 tcgacggttg agttttcggg cacgaagagc acgtcgcgct tgagctcgag catcgtccgt
     3601 cccgggccac gaagctgtgc caggaagaga tccttgacgt gaatgaggcc gatggtctga
     3661 tcgagggtgc cgtcgcagag cggatagcgc gtgtggcgcg tgatgcggat gatctccagg
     3721 ttctcttcga tcgacttgag cgtggagagg acggaaacgt cggtgcgcgg caccatgatc
     3781 tgccgcgccg tccgcttcga gaactggaag acgccctcga tgatctcgcg gttctcttcg
     3841 gagaggattc cggccttctc cgagtgcgcg aggatgaggc gcagctcatc ctgcgagtga
     3901 accatttccg cgccactggc cggcttcacg ccgatgagac gaagcacgcc gttcgaagcg
     3961 cggttcaacg cccagatgat cgggaacatc accaggtaga aagcgcggag cagatgcgcc
     4021 gaccagaggg cggtgccgag ggtcttgtcg atggcgaggt acttcggtcc gagctcgcca
     4081 aagatgatgt gcagagacga gatgacgatg aaggcgacgc tggcggcgat cgtgtgcgcc
     4141 accgagttgc tgtagacgcc gaagaaggaa agacgcggct gcacgagctg cgcgaatgcc
     4201 ggctcgccta cccagcccag cgcgagagag gcgagcgtga cgccgagctg cgtggcggaa
     4261 aggtagctgt cgagcttctt gacgatgcgc cgcgtcatcc gggcgcgcgc gctgccggcg
     4321 gcggccatct ccgcgagctg tgtcggccgc accttgatga tcgagaactc ggcggcgaca
     4381 aaaaagccgt tcgcgatgat gaacagcaag gcgagaagca agtaccaggt aggcatgctc
     4441 accgagcgcg aagggcgaac gcgctacgag atgggtcgac gtccatgaga aggccggtat
     4501 tgtatgccac gtctttcagg cttcaactcg gcggcgcggt cacctcgtcg agcgttttcc
     4561 agaatcgtgg gtacgacttc gataccacct gctcgtcggc gatcgtgacg ttgccgcgcg
     4621 ccagaccggc gatggcgaac gccatggcga tacggtgatc gttgtacgtt gcgaccgtga
     4681 ccggttctga tgtccagccc ggttggatga gtagggagtc cggccgctcc tcgacggtaa
     4741 cgccgaggcg gcggagctcc gaggtgacgg tggcgaggcg gtcggattct ttgacccgta
     4801 aattcgctac gttcacgatc tcgaccggcg atgacgccag
//
LOCUS       DATLVW010000953         7406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_494358, whole genome shotgun sequence.
ACCESSION   DATLVW010000953 DATLVW010000000
VERSION     DATLVW010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494358"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VNN25_28200"
     CDS             <1..1139
                     /locus_tag="VNN25_28200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH95492.1"
                     /translation="LSEALPADGFETLLVTGTVPPGEEDMSAFAAAHGVTPIVIPSMS
                     RELSPRDLSTIWQLWRLMVRFRPDIVHTHTAKAGAVGRIAGLFYRLMSRPSQRCRFVH
                     TYHGHVFHSYYGKWKTRFFLAIERTLARLNTDRIVVLGEQQLREIRDTFRVGRPNQFV
                     VLPLGIDLGELDAEPGSGDVLRAQLGIEAPEIVVGIVGRLTAIKNHDLFLRVATRLGN
                     AARFVIYGDGGDREALEQRAGSNIIFAGTQPATAIYDSLDIAALTSRNEGTPLALIEA
                     MACGRPVISTAVGGVVDLLGEVEERVSDDNGATFEIRERGITAASDDDLGFAAGLARL
                     LRDEPLRQTLAGRGKAYVRKAHSKERLVADMIRLYRGLAAADHA"
     gene            1156..2172
                     /locus_tag="VNN25_28205"
     CDS             1156..2172
                     /locus_tag="VNN25_28205"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXH95493.1"
                     /translation="MRVLITGGAGFIGSHLADACIARGDEVFVIDDLSTGSLKNIEQL
                     ADHPRFHCTIDSVRNEPVLEALVDDCDVIFHLAAAVGVKLIVESPVRTIETNVRGTEV
                     VLAAAERKRKRVLIASTSEVYGLSEQVPFREDGNLVMGATTKGRWSYACSKAIDEFLA
                     LAYHHEKGLPAIIVRLFNTVGPRQIGRYGMVVPTLVSQALAGEPLTVYGNGRQTRCFG
                     YVADVVEALLRLIDHPDTPGQVFNIGSTEEVSITQLAERILALTGSGSRIIYVPYSEA
                     YEEGFEDMPRRVPDTSKAARVIGFRPRTSLDDILRAVIEFRSSREGAEAVLAASSRAR
                     SSAS"
     gene            2169..3995
                     /locus_tag="VNN25_28210"
     CDS             2169..3995
                     /locus_tag="VNN25_28210"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95494.1"
                     /translation="MSPQFLTTAILAAAGAFLSSVALTPMVGAIARRRGAVAKPKADR
                     WHTKPTAMFGGVAIVIAVIGTQLLLLPMTRESRIVMTASVALFLVGLADDLLHIKPYQ
                     KLIGQLIGAAGVVSFGLVLPWTGSFALNVLITMFWLIGITNAVNMLDNMDGLAAGVSA
                     IAASFLGLNFIANQQWPEALMLAAFAGALLGFLIYNHQPASIFMGDCGSMFVGFLLAS
                     SALVSGPGGGRSRSVVAVLAVPVLVFVVPIFDTTFVTLMRKLAGRAASQGGRDHTSHR
                     LVALGLSERHAVWMLYTFAVAAGSLAMLVRHAALDVSISAIASFTLILTFLGVSLAHV
                     RVYDENDAGAARPRALFAFLIDFSYKRRVFEVALDVLLIVLSYYFAHVLVFGPASDSA
                     GWHLFLATLPIAIALKLAALLMSGIYRGLWRYAGLSDVFTYARGVALGSAATIVYVAL
                     IARREGVSLSVFVIDAMLLLLAVTSSRLAFRALRRLFPAARNPTGRRVVIYGATDAGE
                     LLLRQLHLNTALQRIPVAFLDADPRKAGRFVHGLPIGNATGAESIAGFCRQHDAAELL
                     IATLGMPAATLQEIIDACSRAGVAVARMNIDIRELSSSGRAE"
     gene            complement(4030..5835)
                     /locus_tag="VNN25_28215"
     CDS             complement(4030..5835)
                     /locus_tag="VNN25_28215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95495.1"
                     /translation="MILDLLHIPLAVAACFALWKLWTSLAGRGRASLIITLGFLIRAF
                     GGEILFWISYLRLPVARSLQLGDGFWFFAVDGPGMLEYTGQLIAGGPGALLLTGREIP
                     SHAFIQVFTVFAWTLGTFASVAILLNCACFLATCAIILWLGEAGARGERLRLVALAAM
                     AFGPGTILWSLQLLKDTFFILLIAAMIACCFLWQELWRGTLADWRKKAVVAGAMVAVL
                     YMMAGIRWYIAVFFWGAWIVFALMTTLAARRKLAAVLANAVLLVLLAQVVRIGGGSDV
                     PESVRRILDPRPSIAATFRPAALTKEISGRRHGFENTPGGTTILAGRTLEPDAPPGGA
                     ITKQQPAQPALPAPSVQPAPSAQVAPSVQAAPSVPAPTMQSAPTVQQPAPSVQVVPSV
                     QVAPSVQVAPSVAAPAGQPAPAPAVQPAPSVQVAPSVQVAPSVQAAPSVPAPSMQLGP
                     SVQVAPSVQLADRSEQHPLAKTMAGLAAEFLPRSVGQAFGLVRIGGGRGFWLFAEIDT
                     LVLDVLLIFTIVVCARSLRAHRRITPLFVLLLIVFLTTAGPMAYTVNNFGTLFRLRQT
                     IYAILAFLPLTLGSRENVASEPAEPVHHRDAEVAE"
     gene            complement(5854..6963)
                     /locus_tag="VNN25_28220"
     CDS             complement(5854..6963)
                     /locus_tag="VNN25_28220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXH95496.1"
                     /translation="MTRATSDRRLRVLALASYPTEAASSRYRIVQYIEPLAARGIDVT
                     FSPFLDARLFAVLYKPGRLVGRLPALALRTLARIGAVIGSWRADIVLVQREAMLFGPP
                     LIEWIAARLLRRPLVLDLDDATWVSYASPVYGRIATLLKWPSKTNRLIRWASAVACGS
                     PNVASYARSLGASVTLVPTTVDTRVFRPARNAAARVPVIGWIGSHGTYAYLERLFPVL
                     TRLRREVAFTLLIAGAGHDGIDVPGVETIARAWSLETEVADFQSLDIGLYPIAEDEWS
                     AGKSGLKAVQYMACGVPFVMSPVGVCATMGITGETHFAAITDDDWYGHLRALVTDEKL
                     RERMGASGRAFAETHYGIEAGADLLSELILAVART"
     gene            complement(6960..>7406)
                     /locus_tag="VNN25_28225"
     CDS             complement(6960..>7406)
                     /locus_tag="VNN25_28225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXH95497.1"
                     /translation="LTATANKMAEELEQRGFTKDDFRVTQIDPGEIPRYLRAADVALS
                     LILTSFARRSASPTKMAEYFAAGLPVIVTPGVGDVDAHVAECQGGVVLRGFDDAAYDE
                     ALDAIEELRRDPGLAARCQAQARLRYDLGEVGGKRYRRLYEAILRS"
BASE COUNT         1368 a         2391 c         2367 g         1280 t
ORIGIN      
        1 ggttgagcga ggcgcttccg gccgacggct tcgaaacgct gctcgttacg ggaaccgtgc
       61 ctcccggcga agaagacatg agcgcgttcg cagccgcaca tggggtgaca ccgatcgtga
      121 ttccatcgat gagccgcgag ctttcgccgc gcgatctctc tacgatctgg caactctggc
      181 ggctgatggt ccgcttccgc ccggacatcg tgcacacgca cacggcgaag gccggcgcgg
      241 tgggccgcat cgccggtctc ttctaccggc tgatgagcag accatcgcag cgatgccgtt
      301 tcgtgcacac ctatcacggg catgtctttc acagttacta cgggaagtgg aagacgcgtt
      361 tttttctggc gatcgagcgg acgctggcgc gtctcaacac cgaccgcatc gtcgttctcg
      421 gcgagcagca gctccgcgag atccgggaca cgttccgggt cggacgtccg aatcaattcg
      481 tcgtcctgcc gctcggcatc gaccttggcg agcttgacgc ggagccaggc tccggcgacg
      541 tgttgcgcgc gcagctcggc atcgaagcac ccgagatcgt cgtcggaatc gtcggccgtc
      601 tcacggcaat caagaaccac gacctcttcc tgcgcgttgc cacgcgattg ggtaacgcgg
      661 cgcgattcgt catctacggc gacggcggcg accgtgaggc tctcgaacaa cgggcgggga
      721 gcaacatcat cttcgcaggc acgcagccgg ccacggcgat ctatgactcg ctcgacatcg
      781 ccgcgcttac atcgcgcaac gaaggaacgc cgctggctct gatcgaggcg atggcctgcg
      841 gcaggccagt catcagcacg gctgtgggag gcgtcgtcga tctgctcggc gaggtcgagg
      901 agcgcgtcag cgacgacaac ggcgcaacct ttgagattcg cgagcgtggg atcaccgccg
      961 cctccgacga cgacctggga ttcgcggccg gcctcgcccg gcttctgcgc gatgaaccgt
     1021 tgcgacagac actggccggg cgcggcaagg cgtacgtccg gaaagcccac tccaaagaac
     1081 ggctcgttgc cgatatgatc cggctgtatc gtggcctcgc agcggcagac cacgcttaga
     1141 ccgggaaaac cagacttgag agttctcatt acgggcggag cgggcttcat cggctcgcat
     1201 ctcgccgatg cctgcatcgc gcgcggtgac gaggtcttcg tcatcgacga tttatccacc
     1261 ggcagcctga aaaacatcga gcagctcgcc gaccacccgc gcttccattg cacgatcgat
     1321 tcggtccgca acgagccggt tctcgaggcg ctcgtcgacg actgcgacgt gatttttcac
     1381 ctcgccgccg cggtcggcgt caaactgatc gtcgagagtc cggtccgcac gatcgagacg
     1441 aacgttcgcg gaacggaagt cgttctcgct gccgccgaac gcaagcggaa gcgtgtgctg
     1501 atcgcctcga cgtccgaggt ttatggactg agcgaacagg tcccgttccg cgaagacggc
     1561 aacctcgtga tgggagccac gacgaagggg cgctggagct acgcctgctc caaggccatc
     1621 gacgagtttc tggcgctggc ctaccatcat gagaagggac tccccgcgat catcgtgcgt
     1681 ctcttcaaca cggtcgggcc gcgtcagatc gggcgctacg ggatggtcgt gccgacgctc
     1741 gtcagccagg cgctcgccgg cgagcccctc acggtctacg gcaacggacg gcagacgcga
     1801 tgtttcggat acgtcgccga cgtcgtcgag gcgttgctcc gcctcatcga tcatcccgac
     1861 acgccgggcc aggtcttcaa catcggatcg accgaagagg tttcgatcac gcaacttgcc
     1921 gagcggattc tggcgctcac cggatccggc tcgcgcatca tctacgtgcc gtacagcgag
     1981 gcatacgagg aaggttttga agacatgccg cgccgcgtgc ccgacacatc gaaagcagcg
     2041 cgcgtgatcg ggttccgtcc tcgcacgtcg ctcgacgaca tcctgcgcgc ggtgatcgaa
     2101 ttccgttctt cgcgcgaagg tgccgaggcg gttctggcgg catcgtctcg agcgcgaagc
     2161 agcgcctcat gagcccgcag tttctgacga cggccatcct cgccgccgct ggcgccttcc
     2221 tatcgtccgt ggcgttgacg ccgatggttg gtgcgattgc acgccgccga ggtgctgtcg
     2281 cgaagccgaa ggccgatcgt tggcacacga agccgacggc gatgttcggc ggcgtcgcca
     2341 tcgtaatcgc cgtcatcgga acgcagctcc tcctgctccc gatgacgcgc gaaagccgca
     2401 tcgtgatgac ggccagcgtt gcgcttttcc tggtcggact cgccgatgac ttgctgcaca
     2461 tcaagccgta ccagaagttg atcgggcagc tcatcggcgc tgccggtgtc gtctcgttcg
     2521 gattggtgct tccgtggacg gggtcgttcg cgctgaacgt gctgatcacg atgttctggc
     2581 tcatcgggat cacgaatgcc gtgaacatgc tcgacaacat ggacgggctg gccgcgggcg
     2641 tgtccgcgat cgctgcgtcg tttctggggc tgaacttcat cgcaaatcag caatggccgg
     2701 aagccctgat gctcgccgcg ttcgccggcg ctctcctcgg gttcctgatc tacaaccatc
     2761 aacccgcatc gattttcatg ggcgactgcg gatcgatgtt cgtcggcttt ctgctggcgt
     2821 cgagcgcgct ggtcagcggg ccgggcggcg ggcgctcgag gagcgtcgtg gccgtcctgg
     2881 ccgtgccggt gctcgtgttc gtcgttccga ttttcgacac gacgttcgtg acactgatgc
     2941 gcaagctggc tggccgcgcc gcatcgcagg gcggccgcga tcacacatcg catcgcctcg
     3001 tcgcgctcgg tctttcagag aggcatgcgg tctggatgct ctacacgttc gccgtggccg
     3061 cgggctcgct ggcgatgctc gtgcgtcatg cagcgctgga cgtcagcatc agcgcgatcg
     3121 catcgtttac gctgatcctc acgtttctcg gcgtctcgct ggcgcacgtt cgcgtgtacg
     3181 acgagaacga tgcgggcgcc gcacgaccca gagcgctttt tgcgttcctc atcgatttct
     3241 cctacaagcg ccgcgtcttc gaagtggctc tcgacgtgct gctgatcgtg ttgtcgtact
     3301 acttcgcgca cgttctcgtg ttcggaccag cctcggactc cgcgggatgg cacctctttc
     3361 tggccacgct gccgatcgcg attgcactga agctggccgc cttgctgatg agcggcattt
     3421 accgcggctt atggcgctac gccggcctgt ccgatgtatt cacctacgcg cgtggcgtag
     3481 cgctcggctc tgcggcgacg atcgtctatg ttgcgctgat cgcgcggcgt gaaggtgtgt
     3541 cgttatcggt cttcgtcatc gacgccatgc tgctgctcct ggcggtgacg tcgagccggc
     3601 tggcgtttcg ggcactgcgg cggttgtttc cggcggcgcg aaatccaacc ggacggcgcg
     3661 tcgtgatcta cggtgcaacc gatgccggcg agttgctgct tcgccagttg cacctcaaca
     3721 ccgcgctgca gcgcattccg gtggcgttcc tcgacgccga tccgcgcaag gccggacgat
     3781 tcgtgcacgg actaccgatc ggtaacgcaa ccggcgcgga atcaatcgcc gggttctgcc
     3841 gccagcacga cgccgcagag ctcctgattg cgacgctcgg gatgccggcc gcaacgctgc
     3901 aggagatcat cgatgcctgc tcacgcgccg gcgttgcagt ggcccgtatg aacatcgata
     3961 tccgcgagct atcgtcgagc ggtcgggcag aatgaaccac agagacacca aggacacaga
     4021 gtccaaacgc tactctgcga cctctgcgtc tctgtggtgg acaggttctg ctggttcgga
     4081 cgcaacgttt tcgcgcgatc ccagcgtcag cggcaggaag gcgaggatcg cgtagatcgt
     4141 ctggcgaagg cgaaagagcg tgccgaaatt gttgacggtg taggccatcg gtccggctgt
     4201 cgtcaggaaa acgatcagca gcaacacgaa gaggggcgtt atgcgtctgt gcgcgcgaag
     4261 gctgcgcgcg cagacgacga tcgtaaagat cagcaagacg tcgagaacga gcgtgtcgat
     4321 ctccgcgaaa agccagaagc ctcgtccgcc tccgatgcgg accagcccaa aggcctgtcc
     4381 gacgctgcgc ggaagaaact cggccgctag ccccgccatc gtcttcgcca ggggatgctg
     4441 ttctgagcgg tccgcgagct gcacggacgg cgcaacctgt acagacggcc caagctgcat
     4501 ggacggtgca ggcacagacg gggcggcctg cacagagggg gcaacctgta cggacggcgc
     4561 aacctgtacg gacggcgcgg gctgcacggc cggcgcaggg gccggctgcc ccgccggcgc
     4621 agccacggac ggcgcaactt gcacggacgg cgcaacttgc acggacggca caacctgcac
     4681 ggacggcgcg ggctgctgca cggtcggcgc ggactgcatg gtcggcgcag gcactgacgg
     4741 ggcggcctgc acggacggcg cgacctgcgc agacggagca ggctgcacgg acggcgcggg
     4801 caaggcgggc tgcgcgggct gctgtttcgt gatcgcgccg ccaggggggg catccggctc
     4861 gagcgtacgg ccggcaagga tcgtcgttcc tccaggcgtg ttttcgaatc cgtggcggcg
     4921 accgctgatt tccttcgtca gggccgccgg gcggaacgta gcggcaatcg aaggacgcgg
     4981 atcgaggatg cggcggaccg attccggcac gtcggaacca ccaccgatgc ggacaacttg
     5041 cgcgagcaga accaggagga ctgcgttggc gagaaccgcc gcgagcttcc tccgtgccgc
     5101 cagcgtcgtc atcagtgcaa acacgatcca tgcgccccag aagaagacgg cgatgtacca
     5161 gcggatgccg gccatcatgt acagaacggc caccatcgcg cctgcgacga cggctttctt
     5221 tcgccagtcg gcgagcgtcc cccgccacag ttcctgccac aagaaacagc aggcgatcat
     5281 cgcggcgatc aggagaatga agaacgtgtc cttgagaagc tgcagagacc agagaatcgt
     5341 ccccgggcca aaggccatgg ctgccagagc gacgaggcgc agccgctcac cgcgcgcacc
     5401 ggcttccccc agccacagga tgatcgcgca cgtggcgaga aagcacgcac agttgagcag
     5461 gattgccacc gacgcaaacg ttccgagcgt ccaggcaaac acggtgaaca cctggatgaa
     5521 cgcatgcgaa ggtatttctc tcccggtcag caagagcgcg cccggaccgc ctgcgatcaa
     5581 ctgacccgtg tattcaagca tccccggtcc gtcgacggcg aaaaaccaga agccgtcgcc
     5641 gagttgcagc gacctcgcca ccggcagtct cagatacgag atccagaaga ggatctcacc
     5701 gccgaaggcc cgaatcagaa agccgagggt aatgatgagc gatgcacggc cacgtcctgc
     5761 aagcgacgtc cagagcttcc acagggcaaa gcatgcggcg acagcaaggg ggatgtgaag
     5821 aaggtcgaga atcatcaacg gcggcgcagt gtctcacgtt cgtgccacgg cgagaataag
     5881 ctcagaaagc aggtcggcgc cggcttcgat cccgtagtgc gtctccgcga atgcccgtcc
     5941 gctcgcgccc atccgttcgc gaagtttttc gtccgtcacc agcgcacgca gatggccata
     6001 ccagtcatcg tcggtgatcg ccgcgaagtg cgtctcgccg gtgatgccca tcgtcgcgca
     6061 aaccccgacc gggctcatga cgaagggcac gccgcaggcc atgtactgca ccgctttgag
     6121 tccggacttc cccgcgctcc attcgtcctc ggcaatcgga tagagcccga tgtcgagcga
     6181 ctggaaatcg gcaacctcgg tctccagact ccacgctctc gcgatcgtct cgactcccgg
     6241 aacatcgatg ccatcatgcc cggccccggc aatcagcaac gtgaacgcaa cctcgcgccg
     6301 cagccgcgtg agaacaggga agaggcgctc cagataggcg taggtgccat gcgagccgat
     6361 ccaacctatg acgggcacgc gcgcggctgc gttgcgtgca ggacggaaaa cgcgggtatc
     6421 gaccgtcgtc ggaacgagcg tgacggatgc gccgagggaa cgggcgtaac ttgcgacgtt
     6481 cgggctaccg cacgcaacgg cactcgccca tcgaatgagc cggttcgtct tcgaaggcca
     6541 cttcaggagt gttgcaatgc gaccatagac cggactggca tacgaaaccc acgtggcatc
     6601 atcgaggtcg agcacgagcg gacgacggag caggcgcgcg gcaatccact cgatcagcgg
     6661 cgggccaaac aacatggcct cgcgctgcac gaggacgatg tcggcgcgcc acgatccaat
     6721 caccgcgccg attcgcgcca acgtccgcaa cgcgagcgca ggcaaccggc cgacgagtct
     6781 cccaggtttg tagagcaccg cgaaaagccg ggcatcgaga aatggcgaga aggtgacgtc
     6841 gatgcctcgg gcggcaagcg gctcgatgta ttgcacgata cggtatcgcg acgatgctgc
     6901 ctccgtggga tacgaggcca aagcaagaac acggaggcgt cgatccgaag tggcgcgagt
     6961 catgagcgga ggattgcttc gtagagccgc cggtaccgtt tgccgcccac ttccccgagg
     7021 tcgtagcgaa gacgcgcttg cgcctggcag cgcgccgcaa gaccaggatc gcggcgcagc
     7081 tcttcgatcg cgtcgagcgc ttcgtcgtac gccgcgtcat caaagcctcg cagcacgaca
     7141 cctccctggc actctgcaac atgcgcgtcg acgtcaccga cacccggggt gacgatcacc
     7201 ggcaaccccg ctgcgaaata ctccgccatt tttgtcggcg acgccgatct ccgcgcaaac
     7261 gacgtaagta tgagcgacaa cgctacatcg gctgcccgca gatacctcgg gatctctccc
     7321 ggatcgatct gcgtcacgcg aaagtcatct ttcgtgaagc cgcgctgctc gagctcttcg
     7381 gccatcttgt ttgccgtcgc cgtcag
//
LOCUS       DATLVW010000954         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_537537, whole genome shotgun sequence.
ACCESSION   DATLVW010000954 DATLVW010000000
VERSION     DATLVW010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537537"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1001
                     /locus_tag="VNN25_28230"
     CDS             204..1001
                     /locus_tag="VNN25_28230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95498.1"
                     /translation="MKPVILSLVVLFAAAGAFAQPAGFEKVLFPIVIHPLQQTPGEFG
                     TSWVTDISILNVGSSPARLAGSYYCFDCHSEQPLQPGVTYSTVPVPAPGNIGGAFLFV
                     DSAAIGQIRFGLRVRDVSREADGFGSEVPVVRTQGFRAEGVSLLGIPNQPSLRLMLRM
                     YALDGVGGEVRVRIYEQQGGVIHLGDLTGDALFAERTYTLTNIHNSLVPQDQDNYPAY
                     AQAADLPMPSTPLARIDIVPITNGMRIWAFASATNNTTQQVTVISPH"
     gene            complement(1026..1295)
                     /locus_tag="VNN25_28235"
     CDS             complement(1026..1295)
                     /locus_tag="VNN25_28235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95499.1"
                     /translation="MRFTIEQRPLTDPSGRPSTHDPKAVSFHHCEAESAGDAIIRFLT
                     ANAGNIVGDIVKFPGLQAVGTVRLADGVYTLQVTPSSQNLAPIGN"
     gene            1398..>2584
                     /gene="guaB"
                     /locus_tag="VNN25_28240"
     CDS             1398..>2584
                     /gene="guaB"
                     /locus_tag="VNN25_28240"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXH95500.1"
                     /translation="MSDERVLSDLPVIGRIDIHEAERRDPLPSDRFADPLEVGLTFDD
                     VLLRPAKSDIHPNFVEVGTQLTRDIRLNIPIISSAMDTVTEARLAIAMAQQGGLGIIH
                     KNMPIDVQAEEVDKVKRSEAGMIVDPVTMRPWQTITEALEVMAKYKISGVPVTDANGK
                     LVGILTNRDLRFETRFDLPIAERMTKDGLVTAPVGTTLEAAREVLHQHRIEKLLVVDS
                     NGDLKGLITVKDIQKKLRYPNAAKDSLGRLRVGAAIGVGKEGLDRARALIDAKVDVVV
                     IDSAHAHSTGVMEVIRQFKKLFPDTPLIAGNVATYEGASDLIALGVDAIKVGIGPGSI
                     CTTRVVAGAGVPQVSAVIECARAAEKFGIPIISDGGIKFSGDITKAIAAGAHCVMIGS
                     LFAG"
BASE COUNT          503 a          809 c          767 g          505 t
ORIGIN      
        1 gccgcttcga tggctcccga tcagtggcag cctctcttca atcaggccat cgtgctgaac
       61 gatctgcacc gcaccgacga ggcccgcgtc gtcgcggcga agctgcagca gatgaagccg
      121 aacgatcctg atgtgcagaa actgctgact tcgctcgcag caaagcagta gacggcgatc
      181 gtgagcgggc cgtacctgcc ggcatgaaac cggtgatcct ttctctcgtt gttctcttcg
      241 ctgccgctgg agcctttgcg caaccagccg ggtttgaaaa agttctgttt ccgatcgtca
      301 ttcatccgct gcagcaaacg ccgggagaat tcggcacgag ctgggtaacg gacatctcga
      361 tcctgaacgt gggttcttcg ccggcgcgtc tcgcgggctc gtactattgc tttgattgcc
      421 acagcgaaca acctctccaa ccgggcgtga cctacagcac cgttccggtc cccgcgccag
      481 ggaacatcgg cggggcattt ctcttcgtcg acagtgcagc tatcggccag atccgattcg
      541 gcttgcgtgt gcgggacgtc tctcgagagg ccgatggatt cggaagtgaa gtgccagtcg
      601 tgcgcacgca gggcttccgt gcggagggcg tgtccctgct cggcattcct aatcagccga
      661 gccttcgact gatgctgcgg atgtacgcgc tcgatggtgt tggtggcgag gtgcgtgtgc
      721 gaatttatga acagcaaggt ggggtcatcc atctgggtga tctgactggc gatgcactct
      781 tcgcggaacg cacctacaca ctgaccaaca ttcacaattc ccttgtccct caggaccagg
      841 ataattaccc ggcgtacgcg caggctgccg atctgccgat gccatcgacg ccgctggcgc
      901 gcatcgatat cgttccaatc acaaacggta tgcgtatctg ggccttcgca tcggcgacga
      961 acaacaccac gcagcaggtg actgtgatca gtccgcatta gtgtttcggg gtgcggagaa
     1021 actgcttaat tgccgatcgg cgcgagattc tggctcgacg gcgtcacctg cagcgtgtac
     1081 acgccatcag cgaggcgaac ggtgccgacc gcttgcagtc ccgggaactt cacgatgtcg
     1141 ccgacgatgt tgccggcgtt cgcggtcagg aatcgaatga tcgcatcgcc ggccgactcc
     1201 gcttcgcagt gatgaaacga cacggccttg ggatcgtgcg tggaaggacg cccactcgga
     1261 tcggtgagtg gacgttgctc aatcgtgaat cgcatcatca ttgtcgtatt tcgcgcagaa
     1321 acctcattgc gctccgctgt ctccatgcaa tttcggtgcg ctgcgggcct gttctatact
     1381 caacctatgg agctgacatg agcgacgaac gtgtactctc cgaccttccc gtcatcggca
     1441 gaatcgacat tcacgaagcc gagcggcgcg atccattgcc gtccgaccgc ttcgccgatc
     1501 cgctcgaagt cggtttgaca ttcgacgatg tcctccttcg tccagcgaag tccgacattc
     1561 atccgaactt cgtcgaagtc ggcacgcaac tgacccgcga catccgcctc aacattccca
     1621 tcatctcctc ggcgatggat acggtgaccg aggccaggct ggcgatcgcg atggcccagc
     1681 agggcggact cggcatcatc cacaagaaca tgccgatcga cgtgcaggcc gaagaggtcg
     1741 acaaagtgaa gcggtcggaa gcggggatga tcgtcgatcc cgtcacgatg cggccgtggc
     1801 agaccatcac cgaggcgctc gaggtgatgg cgaaatacaa gatctccggt gtgcccgtga
     1861 ctgacgcgaa cggcaagctc gtcggcatcc tcacgaaccg cgatctccgt ttcgaaacgc
     1921 gcttcgacct gccgatcgcc gagcgcatga ccaaggacgg gctcgtcacc gctccggtcg
     1981 gaacgacgct cgaggccgcg cgcgaagtgc tccaccagca ccgcatcgag aaacttctcg
     2041 tcgtcgattc caacggcgac ctcaagggcc tcatcaccgt caaagacatt cagaagaagc
     2101 ttcgctaccc gaatgccgcg aaagactcgc tcggccgttt gcgcgtcggc gcggcgatcg
     2161 gtgtgggcaa agaagggctc gaccgtgccc gtgcactgat cgacgccaag gtcgacgtcg
     2221 tcgtcatcga ctcggcgcac gcgcattcga ccggcgtcat ggaagtgatc cgccagttca
     2281 agaagctctt tcccgatacg ccgctcatcg ccggcaacgt cgccacctac gaaggtgcga
     2341 gcgatctcat cgcactcggc gtcgatgcca tcaaggtcgg tatcgggcct ggctcgatct
     2401 gcaccacgcg cgtcgtggcc ggagcgggcg tgccgcaggt cagcgccgtg atcgagtgcg
     2461 cccgcgccgc ggagaagttc ggcatcccga tcatcagcga tggcggaatc aagttctccg
     2521 gcgatatcac caaagcgatc gccgcaggag cgcattgcgt gatgatcggc tcgctcttcg
     2581 ccgg
//
LOCUS       DATLVW010000955         8915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_452544, whole genome shotgun sequence.
ACCESSION   DATLVW010000955 DATLVW010000000
VERSION     DATLVW010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452544"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..882)
                     /locus_tag="VNN25_28245"
     CDS             complement(301..882)
                     /locus_tag="VNN25_28245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95501.1"
                     /translation="MDSLIWPGTGGLIHEWRLNGARMRLENGTTVRERHSSFETCMPS
                     LRAIMPNVGPPSKEAIEDENLRRAACIFDVTHGTLSAGTLREGGAVFGMLRTETEGSP
                     RLSIRSFGSGLPKEIFLRDGAEITISNLGATEQHDGSHDFYLHYELAATIPTNPAIPA
                     APSSSCVVNKNPRPTWPPGFTSVDVGCSNSAYP"
     gene            complement(870..4313)
                     /locus_tag="VNN25_28250"
     CDS             complement(870..4313)
                     /locus_tag="VNN25_28250"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXH95502.1"
                     /translation="MTDCIDAETLALFAEGKLSRAEIRPILDHVTSCASCMRILKIAR
                     AHAATLEEETAEETPPRTWPYWLAAAAVLALLAIPAALRFRATPVDRLIALAPRSARI
                     VEPRLAGGFAWAPWRGSARAGDVAADAARLKLDGVAGELMERAGEEKSPDAQHAAGVA
                     LLLTERPLDALPRLSDAAERTPGQPRAWSDLAAANYAAASSTGRASLYPEALAAADRA
                     LRIDAKFPEARFNRALILERMGAKDAARTAWERYLEVDPASSWATEAREHLRRLSSIT
                     GELQFRSDLPQLERAAIQDDRTRVAELVAKDPQMSRLYAEGEFLGGWAEAQLRGAAAE
                     AAARLAVARAVGAALERQTGEAMVAGAVRAVDSATADQRNALAEAHAAYRRGRIAYSR
                     NRPADAEADLRRAATGFASAGSPMAYLARYFAACTRYDQNDLVAARAELTSLLAEAGA
                     HPELTALGAQVRWQLSLCFMNDDDWSSALPLLRAAETAFDRLGERNHLGVIRGLLADT
                     LACIGRSDESWSARMQSFALQSAEGRRDQFLAVNFGSAARTALRAGRLNVARSMFDLE
                     IEACRRLKSDVLLTNALARAAVLAAALDDDASASQMVSESTESARRIEDTALRARVLE
                     NAAFASGAAMLRTNPRGARAQLTRAIDGYRNSERAFFLPESYLLRARAARALGDVAAA
                     ADDLERGISELERHRIRYSGTVIGTGVFDAGRSLFHEAIRVSLQRADPARALAYAERS
                     HPQVAGDAPMTVAELQTRLRNTGTSVLEIVALPDETISFCVTAGALRVSRAPIEREAL
                     SGLATRAANGNRDALSRLYAILVQPAEAAILSSDRLIVVSDPVLDAVPFGALYDARSQ
                     RHLIERTQVALALSALSLRDGEPAKTPQSLTMVSLPEGERGGSTGLPHVRDELDDVRR
                     LYAQSFERATFADVVSIRTDVLHIAGHTQVRTGSEEPMLGFAAPHGDAREWMSWRRIA
                     GKPFLSRTTVVLAACETLRRPDSQAFSLSLGGGFLAAGAGDVIGTLAAIGDQDAHRVF
                     YEIHRHLAAGADPAEAVRRAQIDALSRHPTDQLEWFAVGVLTNHIRSSETRGGSRLWV
                     SASTSSVSVRTFGGTRTRRRCPRASYSSTRRKAAPFTRRSTRTFPRSGSPARTSSIWI
                     P"
     gene            complement(4310..5161)
                     /locus_tag="VNN25_28255"
     CDS             complement(4310..5161)
                     /locus_tag="VNN25_28255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95503.1"
                     /translation="MDPRELLESNLEVIDRIAATACRRARRYGPDAEDFAASVRLALV
                     EDDYAVLRKYQGRCAFSTYLTVVIERLMEDDRNRAMGRWRASAEATRLGPAAVLLESL
                     VRRDNRSLEEALPLVQAVDRSLTRERIEATLRQIPERAERPRPMPLDDAVSAVPGSDE
                     TDALALANEARPLSGRTNEVVRETIAALPQEDRALLQFRFAESMKISDISRMMRLPQR
                     PLYRRIEALLDRFRASLAAAGVDGSAALGLLQKTSVDALDFGFEERSSARQSNEEDGP
                     ARAAEPW"
     gene            5287..6411
                     /locus_tag="VNN25_28260"
     CDS             5287..6411
                     /locus_tag="VNN25_28260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95504.1"
                     /translation="MTTISLYSHLTSKQFAEQILDSLERDLDVDLRPIFADNSKSEAQ
                     RRLAVSARLMEALIERAENSPDRAEWISEQDEDHKFLRAFSSGEVDIEPNLCYDVENV
                     GTLKSFRGDEIKDFLYSLTKRFENMSKVTNPAILAAQIVGGGMISVSIPMAAGTIQAL
                     RAGQTLVAAVKAGITNVGMKTAIAAVVIMLAALLIWLFLENPKKFLGMVINQTDSNLV
                     VNDWRKGIDGDTGGDLFMRHGSMVSFPEDYESGRLTKKVQISKMDRIGPKDDLCYAGI
                     FFANKNFGGYGVEGVAIYSAQDGSAKFAQLFACPYSEDNGTAVRVLNGATKDVPALFR
                     DMYPKRKTQDTTSSGGFTLVSTVNDNRGGVVGCITCFSKA"
     gene            6475..7560
                     /locus_tag="VNN25_28265"
     CDS             6475..7560
                     /locus_tag="VNN25_28265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95505.1"
                     /translation="MRNDQEVVHEGRELALQVLARLRDDLGIDLLPLFEDESLPERER
                     LRRARTELFHALCVRGGFDEQAFDIAGVEIEPNTYVTFKEARRLSWLTGPAVFQYISK
                     LTSSMEAASKAAGPESLAHLIIDAGLVSVGSSMMRETYVFLRGGQVLQAALRSGITRI
                     GMASAIGCIALALVALVAWLIAINPKKLLGVIINGTASTIVVKNWRAGVDGAKGSDLF
                     MKHGSMNSFMQDYEAGHLSKVIQINARLDIGEGLDGRICAAGLFFADKNIGVYGAEGV
                     MILTAGDGAFEIAHMFAVPYNKDNGTNISFVRGPFPDLERLFRDLYNQRATRREIFNT
                     NYAASSTINDARGGTVGCIAYVGPTSG"
     gene            7591..8139
                     /locus_tag="VNN25_28270"
     CDS             7591..8139
                     /locus_tag="VNN25_28270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95506.1"
                     /translation="MRSSRPRALGRPRPNAVRAEVEAEYRLTFAEHVRAQLNELNAGL
                     EYQVLAASNSHARQANFITPAAEPLFRRVDLFNAGNLGEGLGIRGCVITMGDIFNGNL
                     SIACTRHTPSGQPNTVPTRGERVDWWKATTAGRVRPDPFQRPVEDLPLPRAVGRSRNV
                     FDRYRAAVPPADHGGQHHPRQG"
     gene            8184..8573
                     /locus_tag="VNN25_28275"
     CDS             8184..8573
                     /locus_tag="VNN25_28275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95507.1"
                     /translation="MPAAVTRFSAAPGSTSPRTTSKWNGGLTEGWNDRYFDAANLRAG
                     MLYRGTLEWNAANAPKDQYIEVNKYSYARSKKHVKYPKGGELTLHGSFGDTIPKVCGD
                     TACADFEQPGPQPPPPSLQAGVRRPLR"
     gene            8551..8844
                     /locus_tag="VNN25_28280"
     CDS             8551..8844
                     /locus_tag="VNN25_28280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95508.1"
                     /translation="MFGGRSADVSVRAALVIDNDGAAGSNGLVWEKKGTSDSGFEDRL
                     KAGNESKRIRLDNSGTYIHFGGGIKMNAQTSTTSKTQWWMFGLVFIENAYLTE"
BASE COUNT         1652 a         2897 c         2922 g         1444 t
ORIGIN      
        1 gagcaatttc cgccatatgg cgaaactcgg tgctgcgcac attacgccat atggtggaag
       61 cgatgtcaag tcactactcg cggctttcag ccgggcggca gatctgtcgc ccgttgcgag
      121 gcgtgaaaat acggtccccc cgcgtgcttc tcggctataa aacgggggct agtcacttga
      181 atctgagggg gggccaatgg gctctcacac ccagcgacct ccggcgagac ggccggagac
      241 gcgggtaata gcaaagggat gtctaccacc gcaagctgtt gggtcatgcc ccgcgcaacc
      301 tcacggatac gccgagttcg aacaccctac atcgaccgaa gtgaatcctg gcggccaggt
      361 tggacgtggg tttttgttga cgacgcagga cgaactgggg gcggcaggga tggcgggatt
      421 cgtcgggatc gtcgcggcca gttcgtagtg gaggtagaag tcgtggctgc cgtcgtgctg
      481 ttcggtcgcg ccgaggttcg agatcgtgat ttcggcgccg tcgcggagaa agatctcttt
      541 cggaagtccg gatccgaacg agcgaatgct caaccgcggg gacccctctg tctccgtccg
      601 cagcatgccg aacaccgcgc cgccttccct gagcgtgccg gcggagaggg tgccgtgcgt
      661 gacgtcgaag atgcaggcgg cccttcgcag attttcgtcc tcgatggcct ctttcgaggg
      721 cggaccgacg ttcggcatga ttgcgcgaag cgagggcatg caggtctcga aggatgaatg
      781 ccgttcgcga acggtcgttc cgttttccag gcgcatgcgc gcgccgttga gtcgccactc
      841 gtggatcagc ccacccgttc ccggccagat cagggaatcc atattgagga tgtcctggcc
      901 ggcgatccgg agcgtgggaa cgtgcgcgtg gatctccggg tgaacggagc tgccttccgt
      961 cgcgttgacg agtacgacgc gcgggggcaa cgccggcgtg tcctcgtccc accaaacgtg
     1021 cgtacagata ccgatgaagt agacgctgat acccatagtc gcgatcctcc cctcgtttca
     1081 gaacttcgga tgtgattcgt cagaacgccg acggcaaacc attccagttg atcggtggga
     1141 tgccgggaca atgcgtcgat ctgtgcgcgg cggacggctt cggccggatc ggcgcccgcc
     1201 gcgagatggc ggtggatctc gtaaaagacg cgatgcgcgt cctgatcacc gatcgcggcg
     1261 agggtgccga tgacatcgcc ggcgcccgcg gcgagaaacc cgccgccgag gctcaacgaa
     1321 aacgcctgcg agtccgggcg gcggagcgtc tcgcatgccg caagcaccac ggtggtacgg
     1381 gaaagaaaag gcttgccggc gatgcgccgc cacgacatcc actcgcgcgc atcgccgtgc
     1441 ggagcggcga acccgagcat cggctcttcc gagccggtgc gtacctgcgt gtgcccggcg
     1501 atgtggagaa catcggtccg aatcgacacg acgtctgcga aggtcgcgcg ctcgaacgac
     1561 tgcgcgtaga gccggcgcac atcgtccagc tcatcgcgca cgtgcggaag tcccgtcgaa
     1621 ccgccacgtt ccccctccgg cagcgagacc atcgtcagcg attgcggtgt ctttgccggc
     1681 tcgccatccc gcaatgagag cgcgctcagc gcgagcgcga cctgcgtgcg ctcaatcaga
     1741 tgacgctggg accgcgcgtc gtatagagca ccgaacggca cagcatcgag cacggggtcg
     1801 gagacgacga tcaggcgatc cgaggagagg attgctgcct ccgccggctg cacgaggatc
     1861 gcgtagaggc gggagagcgc gtcgcggttc ccgttcgccg cgcgcgtggc gaggccggac
     1921 agcgcctccc gctcgatggg ggcgcgcgaa acgcgcagcg caccggcggt cacgcagaag
     1981 gagatcgttt catcgggcaa cgccacgatc tccagtacgg acgtgccggt gttccgcagc
     2041 cgcgtctgca actcggccac ggtcatcggt gcgtcgccgg cgacctgcgg atgcgaacgt
     2101 tcggcatacg cgagagcacg cgcgggatcg gcgcgttgca gggagacgcg gatcgcttcg
     2161 tgaaacagcg atctccccgc gtcaaagacg cccgtgccga tcaccgtacc ggaatagcgg
     2221 atgcgatgac gctcgagctc ggagattccg cgctcgagat cgtccgcggc ggcggcgacg
     2281 tcgcccagcg cccgcgccgc ccgcgcacgg agtagatagg actcgggcag gaagaaggcc
     2341 ctctcgctgt tccggtagcc gtcgatcgcg cgggtgagtt gcgcgcgcgc gccgcgcgga
     2401 ttcgtgcgca gcatcgccgc gcccgacgca aacgcggcgt tctcgagcac ccgcgcgcgg
     2461 agcgcagtgt cctcgatgcg tcgtgcactt tcagtcgatt ccgaaaccat ctgtgacgcg
     2521 gacgcgtcgt cgtcgagtgc ggcggcgagc accgccgcgc gcgccagcgc attcgtcagc
     2581 agcacgtcgc tcttcagccg gcggcaggcc tcgatctcga gatcgaacat cgagcgcgcc
     2641 acattcaggc gtccggcacg caatgccgtc cgcgccgcgc tgccgaagtt cactgcgaga
     2701 aactgatcgc gccgtccctc ggcgctctgc aatgcgaatg actgcatgcg ggcgctccac
     2761 gactcatcgg aacgtccgat gcaagccagc gtgtcggcga gcagtccgcg gatgacgccg
     2821 agatggttac gctcgccgag acgatcgaac gccgtttccg cggcgcgcag cagcggcagg
     2881 gcgctgctcc agtcgtcgtc gttcatgaaa cagagcgaca gttgccatcg cacctgcgcg
     2941 ccgagcgcgg tcaactccgg atgagcgccg gcttcggcga ggagcgaggt cagctccgca
     3001 cgggccgcga cgaggtcgtt ctggtcgtaa cgcgtacacg cggcgaagta gcgcgcgaga
     3061 tatgccatcg gactgcccgc gctcgcgaaa cccgtcgccg cgcggcgaag gtccgcctcg
     3121 gcatccgcgg gacggttgcg gctgtaggcg atgcgcccgc ggcggtacgc ggcgtgcgct
     3181 tcggcaagag cgtttctctg atctgcggtt gcgctgtcga cagcccgcac tgcgccggcc
     3241 accatcgctt cgccggtctg ccgctcgagg gccgcaccga ccgcgcgcgc gacagccaat
     3301 cgcgccgcag cttcggccgc agcgccgcgc aactgcgcct cagcccatcc gccgaggaac
     3361 tctccctcgg cgtagaggcg gctcatctgc ggatctttcg cgaccagctc cgccacgcgc
     3421 gtgcgatcgt cttggattgc agcccgctca agctgcggca gatcgctccg gaactgcagt
     3481 tcgccggtga tcgaggagag tcgacgcaga tgctctcgtg cttccgtcgc ccacgacgac
     3541 gccggatcga cctcgagata gcgttcccac gccgtgcgcg cggcgtcctt cgcgcccatc
     3601 cgctcgagga tgagcgcgcg gttgaaacgt gcttcgggga acttggcgtc tatgcgcagc
     3661 gcgcggtccg cggcggccag cgcctcggga taaagcgaag cgcgcccggt gctcgacgcg
     3721 gcggcatagt tcgccgcggc gagatcgctc cacgcgcgcg gctggcccgg cgtgcgctcg
     3781 gccgcatcgc tgagacgcgg gagcgcgtcg agcggccgct ccgtgagcag aagggccacg
     3841 ccggccgcat gttgcgcgtc gggcgacttc tcctcgccgg cgcgctccat caattcgccg
     3901 gccactccgt cgagcttgag acgcgccgcg tccgccgcga catcgcctgc gcgcgcagac
     3961 ccgcgccatg gcgcccatgc aaaaccgccg gcgaggcgcg gctcgacgat gcgcgcggag
     4021 cgcggcgcga gcgcgatcaa tcggtccacc ggcgtggcgc ggaagcggag cgctgccgga
     4081 atcgccagga gcgcgagaac cgcggccgcg gcgagccagt acggccaggt gcgaggcggt
     4141 gtttcctcgg ccgtctcttc ctcgagcgta gccgcgtgcg cgcgggcgat tttcaggatc
     4201 cgcatgcacg atgcgcacga cgtcacgtga tcgaggatcg gacgtatctc cgcgcggctg
     4261 agctttccct cggcgaacag cgcgagtgtc tcggcatcga tgcaatcggt caccatggct
     4321 ccgcggccct cgccgggcca tcctcctcat tggactggcg cgcgctactt ctttcctcga
     4381 atccgaagtc gagtgcatcg acagacgtct tctgcaacag acccagggcc gcgctgccgt
     4441 cgacgcctgc tgcggcaagc gacgcacgga agcggtcgag cagcgcttcg atgcgccggt
     4501 agagcgggcg ctgcggcaat cgcatcatgc gcgagatgtc ggagatcttc atcgactcgg
     4561 cgaagcggaa ctgcagtaag gcgcgatctt cctgcggaag cgcggcgatg gtctcgcgca
     4621 cgacttcgtt cgtgcgtccg gacagcgggc gcgcctcgtt cgcaagcgcc agagcatcgg
     4681 tctcgtcgga cccaggcacc gcggaaaccg catcgtcgag aggcatgggc cgcggcctct
     4741 ccgcacgttc ggggatctgg cgcagagttg cttcgatccg ttcgcgtgtc agagagcggt
     4801 cgacggcctg cacgagcggc agtgcctctt cgagagatcg attgtcgcgg cgcacgagcg
     4861 actcgagcag cacggccgcg ggcccgaggc gcgtcgcctc ggcagacgcc cgccagcggc
     4921 ccatcgcccg attgcggtca tcctccatga gccgctcgat cacgaccgtc agatacgtcg
     4981 agaacgcaca acggccctgg tatttgcgga gcactgcgta gtcgtcctcg acgagcgcga
     5041 gacgcaccga tgccgcgaag tcctcggcat ccggaccgta gcgccgcgcg cggcggcatg
     5101 cggtggccgc gattctgtcg atgacctcga ggttcgattc gagtagctcg cgcgggtcca
     5161 ttgtttttgg gaagcgagaa aaaggatacg cccccacccg caggcgtgga aaaaaaaagc
     5221 gtccgccagt ccaacccgga tgtctcagca aattgattcg aggaaaaaga aaggaaggac
     5281 aagcacatga cgacaatttc cctgtattcc catctcacca gcaagcagtt cgcggagcag
     5341 attctcgaca gcctggagcg cgacctggac gtggatctgc gtcccatttt cgccgacaac
     5401 tcgaagagcg aagcgcagcg ccgccttgcg gtgagtgcac gcctcatgga ggccctcatc
     5461 gagcgcgcgg agaacagccc tgaccgcgcc gaatggattt cggagcagga cgaggatcac
     5521 aagttccttc gcgccttctc gtccggcgaa gtggacatcg agccgaacct ctgctacgac
     5581 gtcgagaacg tcgggactct caagtccttc cgcggcgacg agatcaagga ctttctctac
     5641 tcgctcacga agcgcttcga gaacatgtcc aaggtaacca atcccgccat tctggcggcg
     5701 cagattgtcg gcggaggcat gatcagcgtc tccattccca tggccgcggg aacgatccag
     5761 gcgctgaggg caggccagac actcgtggcc gcagtgaaag ccggcatcac gaacgtcggc
     5821 atgaagacgg ccatcgccgc ggtcgtgatc atgctggccg cgctcctgat ctggctgttt
     5881 ctcgagaacc cgaagaagtt cctcgggatg gtcatcaacc aaaccgattc gaatctcgtc
     5941 gtcaacgatt ggcgcaaagg catcgacggc gacaccggcg gcgatctatt catgaggcac
     6001 ggctcgatgg tctcgttccc ggaggactac gaaagcggca ggctgacgaa gaaggtccag
     6061 atcagcaaga tggatcgaat cggccccaag gacgacctct gctacgccgg gattttcttt
     6121 gcgaacaaga acttcggcgg ctacggcgtg gagggagtgg cgatctattc ggcgcaggac
     6181 ggcagcgcga aattcgcgca actgttcgca tgcccgtaca gcgaagacaa cggcacggcc
     6241 gttcgcgtgt tgaacggagc cacgaaggac gttccggcac tgttccgcga catgtacccg
     6301 aagcgtaaga cccaggacac aacgagcagc ggcggattca ctctcgtcag tactgtcaac
     6361 gacaaccgcg gtggcgtggt cggctgcatc acctgtttca gcaaggcctg atcttcattc
     6421 agcgtggggc cggcgatcct gccggcccca cattgccaac aaggagccat cgtcatgcgt
     6481 aacgatcagg aagtggttca cgaagggcgc gagctcgcgt tgcaggtcct ggcgagactt
     6541 cgcgatgacc tcggcatcga tctccttccg ctcttcgagg acgagtcgtt gccggagcgt
     6601 gaaaggctcc ggcgcgcgcg tacggagttg tttcacgccc tgtgcgtgcg cggcggcttc
     6661 gacgagcagg cgttcgacat cgccggcgtg gagatcgagc cgaacaccta cgtcacattc
     6721 aaggaagcgc ggaggttgag ctggctcacc ggaccggccg tgttccagta catctcaaag
     6781 ctgacctcgt cgatggaagc ggcgagcaag gccgccggcc cggagtccct cgcgcatctg
     6841 atcatcgacg cgggcctggt gagcgtcggc agttcgatga tgcgcgagac gtacgtgttt
     6901 cttcgcggag gccaggtcct gcaggccgcc ttgcgaagcg gcatcacgcg catcggaatg
     6961 gcatccgcga tcggctgcat cgcgctcgcg ctcgtcgcgc tcgtggcctg gctgatcgcc
     7021 atcaatccga agaagctcct cggcgtgatc atcaacggca cggcctcgac gatcgtggtg
     7081 aagaactggc gcgccggagt cgacggcgca aaagggagcg atctcttcat gaagcacggc
     7141 agcatgaatt cattcatgca ggactacgag gccggacact tgtcgaaggt catccagatc
     7201 aacgccaggc tggacatcgg cgagggcctc gatggacgca tctgcgcggc gggcctgttc
     7261 ttcgccgaca agaacatcgg cgtctacggc gccgaagggg tgatgatcct cacagcaggc
     7321 gacggcgcgt tcgagatcgc gcacatgttc gccgtgccgt acaacaagga caacggaacg
     7381 aacatctcgt tcgtccgcgg gccgttcccg gatctcgaac ggctgtttcg cgacctctac
     7441 aaccagcgcg ccacgcgccg cgaaatcttc aacacgaact acgccgcgtc gagcacgatc
     7501 aacgacgccc gcggcggcac ggtggggtgc atcgcatacg tgggaccgac gagcggatag
     7561 tgatcacgcc cggagctcga cgcctgagga gtgcggtcgt ctcgaccgcg ggcactcggg
     7621 cgtcctcgcc cgaatgcggt cagggcggag gtcgaagccg aataccgcct caccttcgcc
     7681 gagcacgtcc gcgcgcagct caacgaactg aacgccggcc tcgaatacca ggtccttgcc
     7741 gccagcaact cgcacgcgcg gcaggccaac ttcatcactc ccgccgccga gcctctcttc
     7801 cgtcgcgtcg atctctttaa cgccggcaac ctcggcgaag gcctcgggat ccgtggctgt
     7861 gtcatcacca tgggagacat cttcaacggc aacctctcca tcgcctgcac acggcacacg
     7921 ccttccggcc agccgaacac cgtccccacc cgcggtgagc gcgtcgactg gtggaaggcc
     7981 actaccgcgg ggcgcgtacg accagatcca tttcagcgac cagtggaaga tctaccacta
     8041 ccacgtgccg tcggtcggag caggaacgta ttcgatcgat accgtgctgc cgtaccgccc
     8101 gcagatcacg gtggccaaca tcacccgcgg caagggtgac accgagacga gcgttccctt
     8161 cggcagcgtc accgccatcg accgtgccgg cggcggttac gcgtttctca gcggcgcctg
     8221 ggagcacgtc accaaggacg acatcaaaat ggaacggagg cctcaccgag ggttggaacg
     8281 accgttactt cgacgcggcg aatctgcggg ccgggatgct ctatcgcggc accctcgagt
     8341 ggaacgcagc gaacgcgccg aaagatcaat acatcgaagt caacaagtac tcatacgctc
     8401 ggagcaagaa gcacgtcaag tatccgaagg gcggcgaact gacgctgcac gggagcttcg
     8461 gcgacacgat tccgaaagtc tgcggcgaca cggcctgcgc cgacttcgag caaccaggtc
     8521 ctcagccgcc tcctccttct ctccaagccg gtgttcggcg gccgctccgc tgatgtgtcg
     8581 gtgcgcgcgg cgctggtgat cgacaacgac ggcgccgccg gcagcaacgg cctggtgtgg
     8641 gaaaagaaag ggacgtccga ttcgggattc gaagaccgtc tgaaggccgg caacgagtca
     8701 aagcgcatcc gcctcgacaa cagcggcaca tacatccact tcggcggcgg cataaagatg
     8761 aacgcgcaga cgtcgacaac cagcaagacg caatggtgga tgttcggcct ggtcttcatc
     8821 gagaacgcct atctgacgga gtgatcaggg ggcagtctga acgggtagta gaagagaccg
     8881 attgacgcca gcatgagggc gccggcgttc gcggg
//
LOCUS       DATLVW010000956         4830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_577675, whole genome shotgun sequence.
ACCESSION   DATLVW010000956 DATLVW010000000
VERSION     DATLVW010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577675"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VNN25_28285"
     CDS             complement(<1..948)
                     /locus_tag="VNN25_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXH95509.1"
                     /translation="MSIHARVDALLGRLASEPQFPTQGQTIFVDLEHQEVRRAYIPLR
                     VVESLLGGRGVNMFLLHNLLDETLEPIHPDVPLIFGTGILTSMVPSASRGNVTSWSPE
                     SRVLLDSNAGDYFPSFMKLNGIDHLVLYGKAAKWTLLHFEGNEPKFIDAAPWVGLDNL
                     DLRARIAHDFKGVEGKDYGLAAITTAGENLVLCSGIMAGPKAIYARGGPGAKMGGLHL
                     KAVLIQGRAADLTTARPHKLRNRDVALKLLSTSVVKNALKVRGTPFLYKPSRMLGAMG
                     TKNNQETTWTDKLDAENIDPYRPGMEGCFACPVNCRPLND"
     gene            1142..1750
                     /locus_tag="VNN25_28290"
     CDS             1142..1750
                     /locus_tag="VNN25_28290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXH95510.1"
                     /translation="MQREEKSERSRRQVLDAALQLFSHQGFRATTVREIAEAAGVSTG
                     NVYHHFPDKESIFRTLLDEFWRITETKRYPYTRALSIGQFPDNIEQLGLAARDSIREF
                     KPYFALIFVDVIEFEGTHIRKFYGGLGQRFGAFVGEEALGSMKQRLRPSVSPISALLI
                     ASRVFFNYFTIEILFNVPEPFGNPAPQIIKELSDIVRHGILM"
     gene            1836..4736
                     /locus_tag="VNN25_28295"
     CDS             1836..4736
                     /locus_tag="VNN25_28295"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXH95511.1"
                     /translation="MKRFALLILIATSALAQQQNDADYTRQIQQFTTGPQFTTEMVDH
                     LPASARVPTPLKFFGYVAGADGHLTYSQDVNRYMRALEAASPRVKVFSIGKSEEGREM
                     IAVAVASDEAIAHLDHYRDITKRLADPRKLTDDDASKLIASGKPIYYITGSIHSPETG
                     SPEMLMELAYRLAVEDSPLIRKIRDNVIVVITPVVEVDGRDRQVDLARWRDANPNRPV
                     PSLVYWGHYVAHDNNRDNLGLALALSRNVLHTFFEFHPQVLHDLHESVPFMYISTGTG
                     PYNPSLDPLVIDEWQRMAWHEVQELTKRGLPGVWTYGFYDGWAPNYMMWAGQGHNAIG
                     RFYETFGNRFPMTADRVVRGQSDRAWFRPNPPLGTVKWSLRNNINYQQSGLLLALSDF
                     ADRRQHFLDQFYLLGKRSIAKAANEGPAAWVFDGAQKRQGQLTDLMNLLRMHGIEVQQ
                     SDAPFSMAVNWPPPAPKPEGSSERRSDSPVRQSSEAINEKSRTGESDLRSSDARPPKP
                     DPSQPVNFAKGSFIVRMDQPYARLADAMLDVQYVRSDEKVYDDTGWTLGYLKNVDFKR
                     VANPDVLKVAAHEWTGGTATSSSPLILENEADTSLPRAIWSTPQARWAVADEAFTVGA
                     KKYAAGTIVAASSTPSTKTHELHLPRIAVLHTWLRTQDEGWFRLGLESLGIPFTYLST
                     QEVAKMPNLRDRFDVILFAPTGGANSSTEIVNGLPPGPPLPWRKTALTPNLGGTDETD
                     DMRPGLGLSGVDHLTRFVEDGGLLITARDTSIWAVQYGLARWVRVIEPQKLRAPGTIL
                     LASITDKKSPIAAGYDDTMPVYYAGAPIFQVGFGAPPPESRPSGRGGKNDPDVAQGRA
                     FTPMPERPKPAPGEEGFQTPEDAPWNSDYAQIRVEDRPRVIVSFAKQADQLLLSGMLE
                     GGDEIAGKPLVVDSPRGKGHILLFANNPMWRQNTQGSYALVTNAIMNWDHLR"
BASE COUNT          962 a         1576 c         1430 g          862 t
ORIGIN      
        1 atcgttcagc ggccggcaat tcacgggaca ggcgaagcag ccttccatcc cgggtcgata
       61 cggatcgata ttctcggcat caagcttgtc ggtccaagtc gtctcctgat tgttcttcgt
      121 ccccatcgcg ccgagcatgc gcgacggttt gtaaaggaac ggcgtgcccc gaaccttcag
      181 agcgttcttg acaaccgatg tcgagaggag cttcagcgcg acatcgcggt tgcgaagttt
      241 gtgcgggcgg gcggtggtga ggtcggccgc gcggccttgg atgaggaccg ccttgagatg
      301 cagaccaccc atctttgcgc ccggtccgcc gcgcgcataa atcgctttcg ggccggccat
      361 gatcccagag caaagaacga ggttctcgcc cgcggtggta atcgcggcca gaccgtagtc
      421 cttcccttcg acgcctttga agtcgtgcgc gatgcgtgcc cggagatcga gattgtcgag
      481 accaacccac ggcgcggcat cgatgaactt cggctcgttg ccttcgaagt ggagcagcgt
      541 ccacttcgca gctttcccat aaagaacaag gtgatcgata ccgttgagct tcatgaacga
      601 cgggaagtag tcgccggcgt tcgagtccag caacacgcgc gactccggcg accacgatgt
      661 gacgttgccc cgcgatgccg agggcaccat cgacgtcagg atgccggtgc cgaagatcag
      721 cgggacgtca ggatggatcg gttccagggt ctcatcgagc aggttgtgaa gcaggaacat
      781 gttcacgccg cgcccgccga ggagggactc gacgacgcgg agggggatgt aggcgcgcct
      841 cacttcctgg tgctcgaggt cgacgaagat cgtctggccc tgcgtgggaa actgcggctc
      901 ggaagcgagc cgcccgagga gcgcatcgac acgcgcgtgg atggacatga gggtcagagt
      961 ttagaacagg gacggggagg gtgctgttcc gagacgaatg gacaattgac agtggatagc
     1021 gactagcggt gcgaaattca cggcactgtc cgtccactca cacggtatcg tgatcgcccg
     1081 tgcccgcgat cgaggggttg accccatatc cttgcgcggg tagctttcgc gcggggtgtc
     1141 catgcagaga gaggagaagt ctgagcgcag ccggcggcag gtgctcgacg ccgctctgca
     1201 attgttctcg catcaggggt ttcgagcgac gactgtacgc gagatcgcgg aagccgcggg
     1261 cgtctcgacc gggaacgtct accatcactt ccccgacaag gaatcgatct tccgcacgct
     1321 gctcgacgag ttctggcgca tcacggagac gaagcgctac ccgtacacgc gggcgctgtc
     1381 gatcggccag ttccccgaca acatcgagca gcttggactg gccgcgcgcg attcgattcg
     1441 cgagttcaag ccgtacttcg cgctgatctt tgtcgacgtc atcgagttcg aaggaacgca
     1501 catccgcaag ttctacggcg gactcggcca gcgctttggc gccttcgtcg gtgaagaagc
     1561 tctcggcagc atgaaacaac gcctccgccc ttcggtctcg ccgatctcgg ccctgctgat
     1621 cgcctcgcgt gtcttcttca actacttcac gatcgagatc ctcttcaatg tcccggaacc
     1681 gttcggaaac ccggccccgc agatcatcaa agagctttcg gacatcgtga gacacgggat
     1741 attgatgtga gcggatgggt tgccgctcga acccactaac tctgcaacgc agcctccaaa
     1801 accggtaacc ttgcgacttc acgcaaggag gctccttgaa acgtttcgct ctcctgattc
     1861 tgatcgccac cagtgccctg gcccagcagc agaatgacgc cgactacacg cggcagatcc
     1921 agcagttcac gaccggaccg cagttcacga ccgagatggt cgatcacctg ccggcctccg
     1981 ctcgcgtgcc gacgccgctg aagttcttcg gatacgtcgc cggcgctgac ggccatctca
     2041 cctattcgca ggacgtgaac cgttacatgc gtgcgctcga ggcagcatcg ccgcgcgtga
     2101 aggttttctc gatcgggaag agcgaagaag gacgcgagat gatcgctgtc gccgtcgcat
     2161 ccgacgaagc gatcgcgcat ctcgaccact accgcgacat caccaaacgg ctggcggatc
     2221 ctcgcaaact gaccgacgac gacgcatcga agctcatcgc ctcgggcaag ccgatctact
     2281 acatcaccgg ctcgattcac tcgcccgaaa cgggaagccc ggagatgctg atggaactgg
     2341 cctacaggct ggctgtcgag gactcgccgc tcatccgcaa gatccgcgac aacgtcatcg
     2401 tcgtcatcac gccggtcgtt gaagtggacg ggcgcgatcg ccaggtcgat ctggcccgct
     2461 ggcgcgacgc caatcccaat cgtcctgttc cgtcgctcgt ctactggggc cactacgttg
     2521 cccacgacaa caaccgcgac aacctcggac tagccctcgc gctcagccgg aacgtgctgc
     2581 atacattctt cgagttccac ccgcaggtcc tccacgatct ccacgagtcc gtccccttca
     2641 tgtacatctc cacaggcacc gggccgtaca acccatcgct cgatcctctc gtcattgatg
     2701 agtggcagcg gatggcgtgg cacgaagtac aggagctgac gaagcgtggg cttcccggcg
     2761 tttggacgta cggcttctac gatggctggg ctccgaacta catgatgtgg gccggacagg
     2821 gacacaacgc catcggccgt ttctacgaga ccttcggcaa ccgtttcccg atgaccgccg
     2881 accgcgtcgt gcgcggacaa agcgaccgcg cctggttccg tcccaatccg ccgctcggaa
     2941 ccgtgaagtg gtcacttcgc aataacatca attatcagca gagcggcctg ctgctcgcgc
     3001 tctccgattt cgccgaccgg cgccaacact tcctcgatca gttctacctc ctcggtaaac
     3061 gctcgatcgc caaagcagcg aatgaaggac ccgccgcatg ggtcttcgac ggcgcacaga
     3121 agcgtcaggg acagctcacc gatctgatga acctcctgcg catgcatggc atcgaggtgc
     3181 agcagtcgga tgccccgttc agcatggcgg tgaactggcc gccgcccgcg ccaaaacctg
     3241 aaggctcctc ggaacgtagg tcggactctc cagtccgaca gtcttctgaa gcgatcaatg
     3301 aaaagagtcg gactggagag tccgacttac gttcgtccga cgctcgtccg ccgaaacccg
     3361 acccgagcca gccggtcaac ttcgccaaag gcagcttcat cgtccggatg gaccagccct
     3421 acgcgcggct cgcagacgcg atgctcgacg tccagtacgt ccgcagcgac gagaaggtgt
     3481 acgacgacac gggctggacg ctcggctatc tcaagaacgt cgatttcaaa cgcgtggcga
     3541 atcccgacgt gctgaaggtt gctgctcacg aatggacggg aggcacggcg acatcatcgt
     3601 cgccactgat tctcgagaac gaagccgata ccagccttcc gcgcgccatc tggtcgacgc
     3661 cgcaggctcg ctgggccgtc gccgacgaag cattcacggt cggagcgaag aaatacgctg
     3721 ccggcacgat cgtcgcggca tcatcaacgc catccacgaa gacacacgag ctccacctcc
     3781 cccgcatcgc cgtcctccac acctggctgc gcacgcagga cgaaggatgg ttccggctcg
     3841 ggctcgaatc gcttggcatt cccttcacct acctctcaac gcaggaagtc gcgaagatgc
     3901 cgaaccttcg cgatcgattt gacgtcatcc tcttcgcccc gaccggcggc gccaattcat
     3961 cgacggagat cgtgaacggt cttcctcccg gtccgcctct cccatggcgc aagaccgcgc
     4021 tgacacccaa cctcggcggc accgacgaga ccgacgacat gcgtcccggc ctcggccttt
     4081 ccggcgtcga tcatctgacg cgtttcgtcg aagacggtgg cctgttgatc acggcgcgcg
     4141 acacgtcgat ctgggccgtg cagtacggcc tggcgcgatg ggtgcgcgtc atcgagccgc
     4201 aaaagttgcg cgcccccggc acgatccttc tcgccagcat caccgacaaa aagagtccga
     4261 tcgccgccgg gtacgacgac acgatgccgg tctactacgc aggcgcgccg atcttccagg
     4321 tcggattcgg cgcgccgcct cccgagtcac ggccgagtgg ccggggcggc aagaacgatc
     4381 ccgacgtcgc ccagggccgt gccttcacgc cgatgccgga acggccgaag cccgctccgg
     4441 gcgaggaagg attccagacg ccggaggatg caccctggaa ctccgactac gcacagattc
     4501 gcgtcgaaga ccgtccgcgc gtcatcgtct ccttcgccaa acaggccgac cagctactcc
     4561 tctccggcat gctcgaaggc ggcgacgaga tcgccggcaa accgctcgtc gtcgactcgc
     4621 cccgcggcaa aggccacatc ctccttttcg ccaacaaccc gatgtggcgt cagaacacgc
     4681 agggcagcta tgcgctggtg acaaacgcga tcatgaattg ggatcatttg agatgagggt
     4741 gtggggagtg gggggtaggg tgtgggagag tgaatcggac tcggtcaaga cgatcgacga
     4801 gatcatccaa ctctttctcc caccccccac
//
LOCUS       DATLVW010000957        10033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_411862, whole genome shotgun sequence.
ACCESSION   DATLVW010000957 DATLVW010000000
VERSION     DATLVW010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411862"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..599)
                     /locus_tag="VNN25_28300"
     CDS             complement(24..599)
                     /locus_tag="VNN25_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXH95512.1"
                     /translation="MSLPEPSLLTPEEYLDIERKSEIRSEYINGRMIAMWGASWRHSI
                     VSGNLFRELSSQLRGKACEAHTNDLRVKVSPTGMYTYPDIVALCGEAELEDEHLDTLL
                     NPSVIIEVLSDSTEAYDRGEKFAHYRRIDSLREYVLVSQNKIRIELFRRDADQWIFSE
                     VTGPDAVLHLESIDCHVAVSTIYEKVEFETK"
     gene            complement(721..1254)
                     /locus_tag="VNN25_28305"
     CDS             complement(721..1254)
                     /locus_tag="VNN25_28305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95513.1"
                     /translation="MRKTQRIAIAVAAVVAVAGITSIVLRHGNDATTRQQDRAQTVTP
                     QLPDTAIVKAVQDANIRIDGLSATNVGGIVVLKGTADPAAAQQAAAVIHQLGFARVAN
                     LIVPRSAIDDDWMRREAEKRLASTRALDGCTLHVSCAKGILRVEGTSYSDMQAEVARN
                     VLRRLGASEVQVQLTKL"
     gene            1411..1806
                     /locus_tag="VNN25_28310"
     CDS             1411..1806
                     /locus_tag="VNN25_28310"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXH95514.1"
                     /translation="MFAINPTDPAPIWRQIEEGVRRMIALGALQPGASVPSVRDLAKD
                     LRVNPNTVARAYQRLTEGGVLAVKRGEGTYVADVPVQPRKNERNEALRDAAIRYASTA
                     IAVGSELEDAVDEVESAYERVVREHRRKA"
     gene            1803..2702
                     /locus_tag="VNN25_28315"
     CDS             1803..2702
                     /locus_tag="VNN25_28315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH95515.1"
                     /translation="MSDSAIQIADLTVNYGRKVAVDQVSLSVTRGAVYALVGRNGAGK
                     SSLVRCLLGQQKPGDGKVTLFDEDVWSRRSELMQRVGVVSEDADAPPEMSVAAIARFC
                     ARLYRKWDQESVEERLRMFGVPLSSRFGSLSKGQRKQVSLALALANTPELLVLDDPTL
                     GLDVVARKSLFEEVIGDLADRGTTVFITTHDLIGVETFADRVGMIENGKLVLDEEVDA
                     LKSRFRRIRFATDPAALDTTVMRPTVMRAWGGGAEAIVSNYDDIAFEQMRSTSSIGAA
                     EVQAMSLEEIFIAIAGGNNGRNE"
     gene            2699..4522
                     /locus_tag="VNN25_28320"
     CDS             2699..4522
                     /locus_tag="VNN25_28320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXH95516.1"
                     /translation="MRNALVIAAREFEEKRFVAYAAIAFAVLPFVILNLIPMSKGKSP
                     GEAIVILAVILSSGFTVGLGVITGATFIGDVSAGRMSFYFSRPVGSLSIWFGKLTAGI
                     VLIVGCFGIIAAPARFIGNSWASVWDITFAQLSVAVLGAAVALFLIAHVIGTFVRSRS
                     PLIAADFVAAVVCGAVIWLLSLPLALGLAANLVKWLLIPLAIALTIAIVAGGAWQLER
                     GRTDRRRGHLALSHFLWGAMAVALLLAAGFVWWVVSVKPADITDAVVSHSPGTAFMTI
                     AGKTSNRGDYVAAWLMNIDDGRSIRIDAQSARGAVQYTRDGRSALWPVLVGNVAELRL
                     YTVGDAAPIDTGLTVWGDFIFPSDDGGRIATVNQGILSIYDVATRRSFASTRIPQTKF
                     ARGLFVTPDLFRIYLQIDSELRIFELDVRTRALRETGVISSPKYAFLSLEPTAARMVV
                     RVAQSNVVTLNDARTGTVIRTLLTGSRASVSRCLRDGRIVVIETQPGGTVMHLFAPDG
                     SPVRDIALGPSAPAWFAGDDGTHVVLLTAMSGTRNLVSIDVNRGVVERREPNVGQWTQ
                     GGFYELRPAIGPLREVVYQDMQKHVMAWNPTTGASRRIVGG"
     gene            4831..5367
                     /locus_tag="VNN25_28325"
     CDS             4831..5367
                     /locus_tag="VNN25_28325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95517.1"
                     /translation="MRQRIGIARAVLGDRPLLVVDEPTTGLDIEARHRLRSLLTSIAG
                     NRIVILSSHISSDIETTATHILLLARGELRWQGSVDSLIAKARGRVFETIVPDAEVRS
                     LSHQYRIITRVRVPTGIRLRGVDAEGQPLHTAEVKPTLEEAYLSEVSTGAIRRGSFAF
                     VFERAEAKASRAPKCCAT"
     gene            5620..>10033
                     /locus_tag="VNN25_28330"
     CDS             5620..>10033
                     /locus_tag="VNN25_28330"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HXH95518.1"
                     /translation="MLLFGAPLAHAALLSITTTALNPPTATVGVGYAAQQAIAATGGV
                     TPYSWSASGLPNGMAINSSSGALFGTPTVAGTFNFTVTVGDSGSPHQTASKGLSITVS
                     VGSTPALSITTTSLNPPTAAVGVGYAAQQAIAATGGQTPYNWSASGLPNGMGINSSSG
                     AVFGTPTVAGTFNFTVTVQDSSSPQQVASKVLSLPIAGTISVTSFGASPSTINAGQSS
                     TLSWATTNATTVSISGVSGTQPNNGSVSVSPTTTTTYTLTATGFGGTTTAATTVTVTQ
                     VTGALDQLRFSFRNARQRAVNGQPAGNIIFLTKRSQDETFRPPVTASINDQPLTQIGV
                     ADGVLTLSGAAGGRSLNDWFPSGGTVRISITNGDGKHFAVILRRSSTMITVVTPKLDS
                     LSSSSASPAATITLSGSNLSPVALENDVILTLPDGRLFHLPAKNASGGSLDVVIPLLP
                     SVDGLAFYSGSFSLSIGTAPGYVSPPVPGVIVELPQLTTPPGQILTQMLQGLKNSLDA
                     RIPAAGAAMAANGVPAPNVQSLQAFPYVLLSMLGDLRDAALAGTPRSFDLETANGTER
                     VYVDAQTISLIERLLMASDSIRSAQAATLSLHSRARALTSACGPTAEELDILIAATAW
                     EAVDIGSDINKAVVECQVGPGSLATKLAAVLMDIASIVDHVILGLGNFELQSLHVNPE
                     IHVRAGKPTKEFVYGVFQSPLPSYLADETIKKLIERYIDAETGGQIPECASVGVGSLI
                     DWIGSLLDDALKAHGYDIHLPAISLSKVPLSAATTRFSTKAGGPPISVDGPCNGEVDL
                     MTQVTGDAVSAFTLHTDRDSFPLMTDPGDSSHLGSSNNASGVFVVDSVSTTPIAAIDA
                     SYGSKTGVENTMLSLTVPAGSQALVDLSAARSSDPQHGTLTYAWLVDGIASGTGLQST
                     AALAPGSSHSIELIVRNLSGGTASAGLIVAVSYPLGSPTAGFFMEAHGVRVTDGDTLH
                     LTTSDTRTTVTLSAVGRSVDPSGQGLAYSWRVGTIDLTGPDVSASLPVGTFGVTLSAT
                     NTNNVIAKATGTIVITKAQTGVPTISDVEPTVTAQPEFVILVHGQDFAPATVLASVDG
                     PGCAPTCFVANGQLLTKTSTAISFTAHGWQSGAYQITVRNGSSGTLSNATSITVLPAP
                     MSGTLNSPSCTIVSGASSCNVMLTWTTTNPVGTSQVTSNTPSAGTVVGNGNNSSTTVN
                     VPHSSRTFFLYNNAVLLTQSTATSSCISGTSWNGSTCQSSVSPMSGTLTSPSCTIDSG
                     ASSCNVTLTWTTTNPVGTSQVTSNTPSAGTVVGNGNNNSTTANVPYSSRVFFLYNNAV
                     LLTQSTATSSCISGTSWNGSTCQSSVSPMSGTLTSPSCTIASGASSCNVTLTWTTTNP
                     VGTSQVTSNTPSAGTVVGNGNNGTATIDVPYSSRSFFLYNNAVLLAKSTATSSCTSGT
                     NWNGSSCQAAPMSGTLSSPSCTISSGASSCNVSLT"
BASE COUNT         1959 a         3103 c         2971 g         2000 t
ORIGIN      
        1 agggtaaatc tgcgagtcgg cctttacttc gtttcgaact ccaccttctc gtagatcgtg
       61 ctgacggcga cgtggcagtc gatcgattcg agatggagaa cggcgtcggg gccagtgacc
      121 tcggagaaga tccactgatc tgcgtcgcgc cggaaaagct ctatacggat cttgttctgc
      181 gagacgagga catactcgcg caaggagtcg atacgacggt agtgggcgaa cttttccccc
      241 cggtcatacg cctcggtcga atcggaaagc acttcgatga tcactgaggg attcagaaga
      301 gtgtcgagat gctcgtcttc gagctcagct tcaccgcaaa gcgcgacgat gtccggatac
      361 gtgtacatcc cagtggggct gaccttcacc cgaaggtcgt ttgtgtgagc ctcgcaagct
      421 ttgccgcgta actgagaact gagctcccga aagaggttgc cgctgacaat gctgtgccgc
      481 cagcttgccc cccacatcgc aatcatccgg ccgttgatgt actcgcttcg aatctccgac
      541 ttgcgttcga tatcgaggta ctcctcaggt gtgaggaggc ttggttcagg caacgacata
      601 gcctgctaag tatgcgccgc gtccctgaaa gatcaagttg acccgccgca gttttgaggg
      661 gacggtttgc gtcggcttct atatcgcgcg acctcgatac ccgaagtcgc gcgacgtcac
      721 ctacaacttt gtcaactgca cctgtacttc gctggcgccg aggcggcgga ggacgttgcg
      781 ggcgacttcg gcttgcatgt cgctgtacga cgtcccttcg acgcggagaa tgcctttggc
      841 gcagctcacg tgaagcgtgc agccatcgag agcgcgtgtc gatgccagcc gcttctctgc
      901 ttcgcggcgc atccagtcgt catcaatcgc tgagcgcggc acgatgaggt ttgccacgcg
      961 cgcgaagccg agctggtgga tcaccgcggc ggcctgttgc gctgcggccg ggtctgccgt
     1021 tcctttgaga acgacgatcc cgccgacgtt cgttgcggac aatccgtcga tgcggatgtt
     1081 cgcatcctga accgccttca cgatcgcggt atcgggaagc tgcggggtca ccgtttgcgc
     1141 ccggtcttgc tgtcgggtcg tcgcgtcgtt gccatgccgc agaacgatgg atgtgattcc
     1201 ggcgacggcc acaaccgccg cgactgcgat agctatcctc tgggtctttc tcataccccg
     1261 aaactactca aacggcatgc cacttgacag caagtacact ctgacttact gtattaggta
     1321 tctagtacac gttgccgagt gagatcgggt tgttcagggg tgcgagagcg tttcggtttt
     1381 ccactcccaa ccaccaaccc cttacacaac atgttcgcca tcaatccgac agaccccgcc
     1441 cccatctggc gccagatcga agaaggcgtc cggcgcatga tcgccctcgg cgcgctgcaa
     1501 ccgggcgcat ccgtgccgtc cgtccgcgac ctcgccaaag atcttcgcgt caatcccaat
     1561 accgtcgcac gcgcctatca gcggctcacc gaaggtggtg tgctcgcggt caagcgtggc
     1621 gaaggaacgt acgtcgctga cgtgccggtg cagccgagga agaacgagcg gaatgaagcg
     1681 ctgcgcgatg ccgcgatccg ctatgcctcg actgcgatcg cggtcggctc ggagctcgaa
     1741 gatgccgtcg atgaagtcga gtcggcatac gagcgtgtcg tgcgcgagca caggagaaaa
     1801 gcatgagcga cagtgcgatt cagattgcgg atctcacagt gaactacggc cgaaaggtcg
     1861 cggtcgatca ggtgtctcta agcgtgacgc ggggcgctgt ctacgcgctt gtgggtcgca
     1921 acggcgccgg caagtcttcc ctcgtccgct gtctcctcgg ccagcagaaa cctggcgacg
     1981 gcaaggtcac gctgtttgac gaagacgtct ggtcgcgccg ttcggagctc atgcaacgcg
     2041 taggcgtcgt cagcgaagat gcggacgccc cgccggaaat gagtgtcgcc gcaatcgccc
     2101 gcttttgtgc gcgcctgtat cgcaagtggg atcaggagag cgtcgaggag cgcctccgca
     2161 tgtttggcgt accgctgagc agccgctttg gcagcctctc gaaaggtcag aggaagcagg
     2221 tgtcgctggc gctcgcgctt gcgaacacgc cggagctgct ggtcctcgat gatccgacgc
     2281 tcggtctcga cgtcgtcgcg cgcaagtcgc tgttcgagga agtgatcggc gaccttgccg
     2341 accgcggaac gacggtcttc atcacgacgc acgatctgat cggcgtggag acgttcgcgg
     2401 accgcgtcgg gatgatcgag aacggcaagc tcgtactcga cgaagaagtc gacgcgctca
     2461 aatcgcgctt ccgacgcatc cgcttcgcca cggatccggc cgcgctcgac acgaccgtga
     2521 tgcgtccgac agtgatgcga gcctggggcg gcggtgccga ggcgatcgtc agcaactacg
     2581 acgacatcgc cttcgagcag atgcgcagca cgagcagcat cggcgcggcc gaggtgcagg
     2641 cgatgtcgct ggaagagatc ttcatcgcca tcgccggcgg gaacaacggg agaaatgaat
     2701 gagaaacgca ctcgtcattg cggccagaga attcgaggag aagcgcttcg tggcctacgc
     2761 ggcgatcgcg ttcgcggttc tgccctttgt cattctcaac ttgattccga tgagcaaggg
     2821 caagtcgcca ggcgaggcca tcgtgatcct ggcggtcatc ctgtcgagcg gcttcacggt
     2881 cggactcggc gtgatcaccg gcgccacctt cattggtgac gtctccgcgg gacggatgtc
     2941 gttctatttc tcgcgaccgg tcggatcgct gtcgatctgg ttcggcaagc tgaccgccgg
     3001 catcgtgctc atcgtcggct gcttcggcat catcgccgcg ccggcgcgct tcatcggcaa
     3061 ctcgtgggcg agtgtctggg atatcacgtt cgcgcagttg agtgtggccg ttttgggagc
     3121 ggccgtagcg ctctttctga ttgctcacgt catcggcacg ttcgtccgtt ctcgctcgcc
     3181 gctgattgcc gcggattttg tcgcggccgt cgtttgcggc gccgtgatct ggcttctcag
     3241 cctgccgctc gcgctcggcc tagccgcgaa cctcgtcaag tggctgctga tccctcttgc
     3301 aattgcgttg acgatcgcca tcgtcgccgg cggagcgtgg cagctcgagc gcgggcgcac
     3361 cgaccggcgg cgcggccatc ttgcgctgtc ccactttctt tggggcgcta tggccgttgc
     3421 gctgctcctc gcggcgggat ttgtgtggtg ggttgtctcc gtgaagccgg ctgacattac
     3481 ggacgccgtc gtctcgcact cgcccggaac agcgttcatg acgatcgccg gaaagacctc
     3541 gaatcgaggc gattacgtcg ctgcctggct gatgaacatt gatgacggac gaagcatccg
     3601 catcgatgca cagtcagcgc ggggagcggt tcaatacacg cgcgacggtc gctctgccct
     3661 gtggcctgtt ctcgtgggaa atgtagccga gttgcgcctc tacacagtcg gcgacgcggc
     3721 accgatcgat acgggtctca ccgtttgggg cgatttcatt tttccgtccg atgatggagg
     3781 gcgaatcgcc acggtcaatc aaggcatcct ctcgatctac gacgtggcca cgagacgttc
     3841 gttcgcatcg acgcgcatcc ctcagacgaa gtttgcacgt ggtcttttcg tgacgcccga
     3901 tctcttccgc atttacctgc agatagattc cgagctccgc atcttcgagc tcgatgtgcg
     3961 aacacgagca ctgcgcgaga ccggcgtgat cagctctccg aagtacgcct tcctttctct
     4021 cgagcccacc gccgcgcgca tggtcgtgcg cgtggcgcaa tcaaacgtcg tcaccctaaa
     4081 cgacgcgcgc acgggaacgg tgatccgcac gctgctgacc ggatcgaggg cttcggtgtc
     4141 gcgctgcctt cgcgatggac gcatcgtcgt catcgaaacg cagcccggcg gcaccgtcat
     4201 gcacctcttc gcgcccgacg gttcgcccgt tcgagacatc gcgctcggac ccagcgcgcc
     4261 cgcgtggttc gccggcgacg atggcacgca tgtcgtgctg ctgaccgcga tgtccggcac
     4321 gcgcaacctc gtctcgatcg acgtgaatcg tggcgtggtc gaacggcgtg agccgaacgt
     4381 cggccaatgg acgcagggcg gcttttacga gcttcgcccc gcgatcggtc cgctgcgcga
     4441 ggttgtctac caggacatgc agaagcatgt gatggcctgg aatcccacaa cgggtgcgag
     4501 tcggaggatc gtgggcggat agaccgagac atcgcgccat gccacactcg tcgagcgcgc
     4561 cttcgctgcg cagccgacgc ggaaggatca cctggtgcgg agtcccgatc agcgccgaaa
     4621 acatcgccgc gttccgccgg tcgatcggat tcctcccgca agagttcacc gcctacgcag
     4681 gcctgaccgc aacgcaattc ctcgacttct gggccctcga gcgcggcatg gaccggcccc
     4741 cgcagcggca cagacagatc gaagagctgc tcgtcgccgt cggactcgaa gagcacgaca
     4801 accgccgcgt ccgcgatttc tccggcggca ttgcgccagc gcatcggcat cgcccgcgcc
     4861 gtcctcggcg accggccact cctcgtcgtc gacgaaccaa ccaccggact cgacatcgaa
     4921 gcccgccacc gcctccgcag cctcctgacc tcgatcgccg gaaaccggat cgtgatcctc
     4981 tccagccaca tctccagcga catcgagacc accgccacgc acatcctcct cctggcccgc
     5041 ggcgagctac gctggcaagg ctccgtcgac tccctcatcg ccaaagcccg aggccgcgtc
     5101 ttcgaaacga tcgtccccga cgccgaggtg cggtcgctct cccatcaata ccgcatcatc
     5161 accagagtgc gagtccccac cggaatccgc ctccgcggcg tcgacgcgga aggccagcca
     5221 ctccacaccg cagaagtgaa acccaccctc gaagaggcct acctcagcga ggtctcgacc
     5281 ggcgcgataa ggagggggag cttcgcgttt gtgtttgaga gggcagaagc gaaagcgtct
     5341 cgggctccca agtgctgcgc gacgtgaacg aggtacttgc caagcctcgg aatttccaaa
     5401 tttcgccatc ccgatagctt tccgtactaa gtgatagagt gcgcaacgag agtagagatc
     5461 ggcacgccat acccatagtt cgttagccct ttcaggggcc gaggtttgcc cgctggcgac
     5521 gggctggagg tagctatgca gtcttctctt cggttgcggt tctcaatcga gcggagttgg
     5581 tcaccagaaa caacacgcgc gtgtcttctt gctttcctga tgctcctttt tggagcacct
     5641 ctagcgcacg cggccctcct ttcgatcacc accaccgcgc tcaacccacc gacagcgact
     5701 gtcggagtgg gttatgcggc gcagcaggcc atagcagcca ctggcggagt gactccgtat
     5761 agttggtcgg caagcggcct gcccaatggc atggcgatca attcgtcgtc aggcgccctg
     5821 ttcggaacgc cgacggtcgc tggtaccttc aacttcacgg tcaccgtcgg ggatagcggc
     5881 agtccacatc aaaccgcctc aaaggggctg tcgattaccg tctcagtcgg ctccacaccg
     5941 gcgctttcga tcacaacaac gtcgctcaac ccgcctacag cagccgtcgg agttgggtac
     6001 gcagcacagc aggcgattgc tgcaaccgga ggtcagaccc catacaactg gtcggcaagc
     6061 ggcctgccca atggcatggg cattaattcg tcatcaggag ctgtgttcgg aacgcccacg
     6121 gttgccggta ctttcaattt cacggtcacg gttcaggaca gcagcagccc gcagcaggtg
     6181 gcctcgaagg tcctgtcgct ccccattgcc ggtactatat cggtcacctc gttcggggcc
     6241 agcccatcaa caataaatgc aggtcagtct tccacgctca gctgggccac cacgaacgct
     6301 acaacagtct cgatcagcgg agtctccggg acgcaaccaa acaatggctc cgtctcggtt
     6361 agtccgacta cgacgacaac ctacacgctt accgcgacgg gatttggcgg gaccacgacc
     6421 gcggcgacaa cggtcaccgt cacccaagtg acgggagcgc tcgaccaact tcgattcagt
     6481 ttccggaacg cccggcaacg cgctgtaaac ggtcagccag caggcaacat catcttcctc
     6541 acaaagagat cacaggacga gacttttaga cctcctgtca ccgcctcgat taacgaccaa
     6601 ccgttaacgc agatcggcgt agccgatggc gtcctcacac tctcgggtgc agcaggtggg
     6661 agatcgctca atgattggtt tccgagtggg gggaccgtcc gaatcagcat cactaacggc
     6721 gacggcaagc atttcgcggt gattctgcga cggtcatcga cgatgatcac tgtcgtgacg
     6781 ccaaaactgg actccttgag ttcctcctct gcatcgccag ccgctaccat cactcttagt
     6841 ggaagcaatc tgagcccggt tgccttagag aatgacgtca ttctgacgct ccccgacgga
     6901 aggctctttc acctcccggc gaagaatgcc tccggaggat ctctcgatgt tgtgatacca
     6961 ctccttccta gtgtggatgg gcttgccttc tatagtggaa gcttctcact atccattgga
     7021 acagcgccgg ggtatgtctc accaccggtg ccgggcgtta ttgttgagct cccccagctc
     7081 actacaccac ccggccaaat tctcacacag atgctgcaag ggctcaagaa ctcactggac
     7141 gcgcgaattc cagctgcggg cgcggcaatg gcagcgaatg gcgtaccggc gcccaatgtc
     7201 cagtcgctac aggctttccc gtacgtcctc ctgtcgatgt tgggcgatct gcgtgatgcc
     7261 gcacttgcgg gaactccgcg atcctttgac ttggagactg cgaacgggac tgagcgcgtt
     7321 tacgtggatg cgcagaccat cagcctgatc gagcgtctac ttatggcctc ggactctatt
     7381 cgcagtgcgc aagcggcgac tctatcattg catagccgcg cgcgggcgct gacttcggcg
     7441 tgtggtccga ctgctgagga actcgatatc cttatagcgg caaccgcgtg ggaagctgtc
     7501 gacattggtt ccgacatcaa caaggcagtt gttgaatgcc aggttggtcc cggaagcctt
     7561 gccacgaaac tagccgccgt cctaatggat atagcatcca tagtggatca cgtgatactt
     7621 ggtctcggca attttgaact gcaatcgctt cacgtgaatc ccgagattca cgtgcgcgcc
     7681 ggcaaaccga ccaaggaatt cgtatacggc gtgttccagt caccattgcc gagctatctc
     7741 gccgacgaga ccataaagaa attaatcgaa agatacattg acgcagagac cggggggcag
     7801 attcccgagt gcgcctccgt tggggtgggt tcgttgatcg actggatagg atcgttgcta
     7861 gacgacgccc tcaaggccca tggttacgac atccatctac cggccatcag cctaagcaag
     7921 gtacctctct ctgcggctac gactcgattc tcaacgaaag caggtggccc gcccatttcg
     7981 gtagatggtc cttgtaacgg tgaagtggac cttatgaccc aggtaacggg cgacgcggtg
     8041 agcgcattca cgctccacac cgatagggat tcatttccgc tcatgactga tccgggcgac
     8101 agttcgcatc tgggcagcag caacaacgca tcaggtgtct ttgtcgtgga cagtgtctcg
     8161 actacaccga ttgccgcgat tgacgcatca tacggttcga agacaggcgt agagaatact
     8221 atgctgagcc ttacggtgcc ggcgggtagc caagcgctgg tcgatttgtc cgcagcacgg
     8281 agctcagacc cacaacatgg gacgcttacg tatgcttggc tggtagatgg cattgcctca
     8341 ggcactggac tccagagcac ggctgcgctg gcgccggggt cgtcccatag catcgaactg
     8401 atcgtcagga acttgagcgg cggtacggcg tcagctggct taattgtagc ggtatcttat
     8461 ccgctgggct cgccgacggc cggattcttt atggaagcgc atggtgtaag agtaaccgac
     8521 ggcgatacat tgcacctgac gacatctgat acgcggacca ccgtgacgct ttccgcggtt
     8581 ggtagaagtg tcgatccgag cggacaagga ctggcgtact cgtggagggt ggggacgatt
     8641 gatctgacgg gcccggacgt gtcagcttcg ctaccggtcg gtacatttgg cgtgacactg
     8701 agcgcgacca acacgaacaa cgttatcgcc aaggcaaccg gcacgatcgt gattacaaaa
     8761 gctcagaccg gtgttccaac gataagtgac gtggaaccca ccgttactgc tcagcccgag
     8821 ttcgtcatcc ttgtccacgg gcaggatttt gcgcccgcca cggtattggc aagtgtagac
     8881 ggcccgggat gcgcgccgac gtgttttgtt gcgaatggac agttgttaac gaaaacgtcg
     8941 acggcgatct cttttacggc gcatgggtgg cagagcggtg cgtatcaaat cactgttagg
     9001 aatggatcaa gtggcacgct gtcaaacgca acatcaataa ctgtgctgcc ggccccgatg
     9061 tcggggaccc tcaactctcc ttcgtgcacc attgtttcgg gtgctagcag ttgcaatgtc
     9121 atgctgacgt ggacaaccac caacccggta ggaacttcgc aagtgacgtc aaatacgccc
     9181 agcgctggga cggtcgttgg caacggcaac aacagctcga ccacggtcaa cgtgccccac
     9241 agcagccgga cgttcttcct gtacaacaac gcggtgctct tgacgcagag tacggccacg
     9301 tcgagctgca tttcggggac gagctggaac gggagcacgt gtcaatcgtc ggtttcgcca
     9361 atgtccggaa ccctcacttc tccttcatgc accatcgact ctggagccag cagctgcaac
     9421 gtgacgctga cgtggacgac gaccaacccg gtaggaacat cacaggtgac gtcaaatacg
     9481 cccagcgctg ggacggtcgt tggcaacggc aacaacaact cgaccacagc taacgtgccc
     9541 tacagcagtc gggtgttctt cctgtacaac aacgcggtgc ttctgacgca gagcacggcc
     9601 acgtcgagct gcatttcggg gacgagctgg aacgggagta cgtgtcaatc gtcggtctcg
     9661 ccgatgtccg ggacgctcac gtctccttca tgcaccatcg cctccggtgc cagcagttgt
     9721 aacgtcacgc tgacgtggac gacgaccaac cccgttggaa cctcgcaggt aacatcaaac
     9781 acacccagcg caggcacggt ggtgggcaat ggcaacaacg ggacagcgac gatcgatgtg
     9841 ccctacagca gcaggtcgtt cttcctgtac aacaacgctg ttctgcttgc gaagagcacg
     9901 gccacgtcga gctgcacgtc ggggacgaac tggaacggga gcagctgtca ggctgcgccg
     9961 atgtcgggga cgctctcgtc gccttcgtgc accatttcct cgggtgccag cagttgtaac
    10021 gtcagcctga cgt
//
LOCUS       DATLVW010000958        11535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_494878, whole genome shotgun sequence.
ACCESSION   DATLVW010000958 DATLVW010000000
VERSION     DATLVW010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494878"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1566
                     /locus_tag="VNN25_28335"
     CDS             85..1566
                     /locus_tag="VNN25_28335"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXH95519.1"
                     /translation="MTTMESPATTQYPDVRNYIGGTFVEGADRYLDVFNPADGSIISR
                     VPLSAASDVDRAVAAAKKAFPGWSATPIKERVQVFYRFKALLEQNIDELARLVTAENG
                     KIDSEARAEVLKSAELCEFACSLPQITAGEVLEVSRGVECRVEHFPVGVVASITPFNF
                     PNMVPNWTIPNAIALGNCMVLKPSELVPISSGRIAELLRDAGLPDGVLNIVHGGQETV
                     EAICDHPGIEGISFVGSTKVAKIVYRRGTSNYKRVLALGGAKNHLIVMPDADPEMTSS
                     NVVASMSGCAGQRCMAASVMMAVGKSDHIIERMVSIAQSMQPGVHIGPVISAAAKARI
                     ESYITEAEKMGAKVLVDGRGYKVKGKENGYYVGPTLIDRVTPDMRIAQEEVFGPVMVI
                     IRANDVAEAVGVQQRSPYGNAASVFTESGGVARYVMENASAGMVGVNVGVPVPREPFS
                     FGGWNDSKFGVGDITGRGSIEFWTKSKKMTTKWNKEAGVNWMS"
     gene            2316..2509
                     /locus_tag="VNN25_28340"
                     /pseudo
     CDS             2316..2509
                     /locus_tag="VNN25_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonate-semialdehyde dehydrogenase (CoA
                     acylating)"
     gene            2580..3350
                     /locus_tag="VNN25_28345"
     CDS             2580..3350
                     /locus_tag="VNN25_28345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95520.1"
                     /translation="MRLWLFVIVLFSCVVANAAYVFVTPNNLGSWHNGPLLVCGAPPG
                     TVDFVLGPGTPPLGSGSLQIDIPSNSPLVVFFGSALDGVPLTDLTELRYITYVVISSS
                     PGIAPRLLLSIDTNNPSAPSDQIEFQPRLQDGSTPGIPNQGAVVTGAWQSWNALAGGW
                     RSLFGPINAPLVTLATYAAAHPGARLQSGNLGPGMAILAGCDGARMLANVDDFRIAVN
                     GAQTIFDFEVFPPVPAFEPWVAALLAIVLVVLGLHKMS"
     gene            3366..4001
                     /locus_tag="VNN25_28350"
     CDS             3366..4001
                     /locus_tag="VNN25_28350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95521.1"
                     /translation="MSDNKTWGSTVMGWFVVKDQAEGSDEPVPAAADDAAAAAPQADV
                     PVFSTPPPPAPGGKVDFDQVFEAAGIDADERQRVSRTLELLTSLPPDTEAAVKKQIVM
                     ASLRAFGVPIEKIIEAGAQELQALDGYIRAGAADTDKVTSEADQRIKQYEEEIVKLKT
                     VMQDRVAEQQSVVSSCNEKKLEVQKVLEFFGQEAVARVVRESPKLHEPSGS"
     gene            4013..4705
                     /locus_tag="VNN25_28355"
     CDS             4013..4705
                     /locus_tag="VNN25_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HXH95522.1"
                     /translation="MFSRLGNLWKGFLSLWIADIEQDHPEIAYENAINSMIAKYAKLK
                     GATAAIIRRREDLDDRFKKANADLAQTTAELNTAVETNQDDLAVILIQKKNQLTADIA
                     EMQTDLQTAHSDAESAKTSLLGVQSEIKKLQAERETMLAKMQSAQARIKIQEQLDGLS
                     VDDEVRALDNVRTHIKNTIAEANLGKELSDSSLDSRLAGLRSQVGDVQAKQELAEMKA
                     RKAAQQQGQKTM"
     gene            4776..6410
                     /locus_tag="VNN25_28360"
     CDS             4776..6410
                     /locus_tag="VNN25_28360"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HXH95523.1"
                     /translation="MKLTPFAKFFITVVVLAVVGYAAWHYKGAEIRRWATGSDKGQSQ
                     TSTSAVGSNDFDALKNAPADPSRDAAVTGVSAVSVNGGKLNRPLVVAINTWAGHSPGI
                     VFNNGLEPNDASNFRKKYGLDVKFVLIEDPASKLAAFRKGDVDIMWNTVDNWAREASI
                     LAENGQKAKSIIMQDWSRGGDGIVSLASINSIEQLKGHRIACTQFTPSHFLLLYLLSQ
                     SGLSADDRAAVEKNIIFTQDAPAAAAMFKAKQVDAAVTWEPDLSGAVTARGDEAHVLV
                     STTAATNIIADTLCARQDVIDKAPESVRDFVHGWLDGIEMMKNDPANSYAIVGKALKL
                     DTDTVSGMLSGLKLTTYADNAQFYGLSGGKAHYDTLFETAFVIWRKKGLVTRSVEAKD
                     WVDTRFLNALAANYGTQKVEEPKIAAKAPSANDRAIINKQIEVHFTPGSDDIMPGSFF
                     VLDSLGDTMTSFGNTYLRVEGNTDATGSPTKNMTLSERRALAVKNYIVKTFPNIDPNR
                     FQTIGRGSTNPVADNSSEAGRQLNRRTEIKVVLTTQ"
     gene            6410..7219
                     /locus_tag="VNN25_28365"
     CDS             6410..7219
                     /locus_tag="VNN25_28365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXH95524.1"
                     /translation="MTAHRRNSIWSIRQPVPRGTALALAFVMPIVVIGAWSIVSYGHL
                     APADFLPSPSEVIRGTLQLFIQYELFTSIVISTQRIVTAFLLASAVAIPLGILMGAFN
                     PINHLFDSIVAPLRYMPISAFIPLLILWFGIYDQEKIAFLFLGVFVYLLPVVVSAIRA
                     VPEELVQTALTLGASKWQVVRTVLLPAALPEIFESFRVMNAILWTYVILAEAVNPEHG
                     LGYMVELARTHQKASWSFAGLIVIGGIGLITDQVIRMISALLFRWKEDADG"
     gene            7212..8087
                     /locus_tag="VNN25_28370"
     CDS             7212..8087
                     /locus_tag="VNN25_28370"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXH95525.1"
                     /translation="MADEQPQAGEPGTVAVTVDAKSSKATYVSERSEQARLRITDLGV
                     TYVGHDGRETEAVREVTLDIVDKPNVGEIVVFLGPSGCGKSTILKSVAGLLAPTRGEI
                     LIDGVPVTGVGRDRGMVFQAYTSFGWLTVRQNVEYGLRLQGLDAKERATRSKKYLEAV
                     GLADFADRYPKDLSGGMKQRVAIARTLINKPKLVLMDEPFGALDPQTRWGMQSLLLDV
                     SRDEDNTILFVTHDVSEAVYLADSIYVMAPRPTKILHRVDVPFFQNRDMALKSSKEFR
                     AVEQKLLDFLYAPAS"
     gene            8097..9044
                     /locus_tag="VNN25_28375"
     CDS             8097..9044
                     /locus_tag="VNN25_28375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95526.1"
                     /translation="MPDPPKKDASPLQTVTPQRAPGQTAAAKPVAKPAQPQADLGRAI
                     RETATDAVKEAAFHNRPYLKMAFANPYNLSLLLGGLTASVLTANPLPGVIALGAEALW
                     LLHGSDSQYLRRILWDPRLEKIRLAMDEQDRNEKMKDLPDSEKERVNGLVAREQQINK
                     LASANPSFTGDLLRIELVKTHKLVESFLEMALTVNRYENYLGTVDVDALERDRERWHD
                     RSIAPQASAPERDIALKNFAVIQKRVERVHEIGRYLGVARAQLDLIENSFQLIADQIV
                     TMQSPNELSGQLDDLLDGVDSIRQTAIDTEKLLNGTESV"
     gene            9041..10870
                     /locus_tag="VNN25_28380"
     CDS             9041..10870
                     /locus_tag="VNN25_28380"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXH95527.1"
                     /translation="MTKAILPKWADELRSRYLRGEASIFVLHGNVYDAVISDGRIVTL
                     TEFLGEVLLDQSKETIAVYNVATGIRFLKRAPTVNIDEALLSGDKPRVLAALERMLVG
                     GTRTAVILEYAEAIAPAGDPNFQSEADRAALVTLHRWSFLEEIERSDNIVILIAENLT
                     ELATKLVSNPRVAVVELPMPDRDERNAAVRVADKRLDDKDVARYGEITAGLKSIQIIS
                     ILTPPPATEEESRQREDFITSILTGPDTAERAKKIAALTAGLSHDEIAKLIAPGAQPA
                     SIPQDRDARARKEVDTLIAHRKREILERECFGLIEFVEPAHGFEVVGGMDEVKKDLLV
                     IANSIRNGETARVPMGVLFTGPMGTGKTFVAEAFARECGLTTIKLKNFRSKWVGATEG
                     NLEKILNVIRAIGQVVVIIDEGDRAFGNTDGEGDGGTSSRVIARIKEFMSDTSNRGRI
                     LFLVMTNRPDKLDVDLKRAGRLDRKIPFLYSQSAEEVEAVANALLKKNAIVTAVNFIA
                     LRENFSQKLIGYSNADVEAVILMANDDAARAGATTVSADNFTHAASDYFPSRDSEMLE
                     YMELLAVFESSSRRLLPPKYAAMTPDELDLRMRALRAVVGSRR"
     gene            complement(10884..11357)
                     /locus_tag="VNN25_28385"
     CDS             complement(10884..11357)
                     /locus_tag="VNN25_28385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95528.1"
                     /translation="MEASELLQNLRLIVRDEMTPFMERMEAGFDRVNSLFDGVYVRLD
                     RLETEYQALRAGLARLEARLDRVESRLGNVESRLSNVESRLESVESRLGCVESELQLI
                     KVALARLEARVSAIETRLADVEGKVDQLASQSEIIELKQEIAMHKARIAKLEARR"
BASE COUNT         2342 a         3551 c         3422 g         2220 t
ORIGIN      
        1 gcccgctcgg aatgacagct cgtcaaattt gattcttcgc gctacccgga tagagttcgg
       61 agcggaatac ccgaggagac ttttatgacc acgatggaaa gccctgccac cactcagtac
      121 cccgacgtcc gcaactacat cgggggcacg tttgtcgaag gggcggatcg ctacctcgac
      181 gttttcaacc ccgccgacgg atcgatcatc tcgcgtgtgc cgttgtcggc cgcaagcgac
      241 gtcgaccgcg ccgtcgcggc cgcgaagaaa gcgtttcctg gctggtccgc gactccgatc
      301 aaggaacgcg tccaggtttt ctatcgattc aaagcgctcc tcgagcagaa cattgacgag
      361 ctggcccgcc ttgtgacggc cgagaacgga aagatcgaca gcgaagcgcg cgccgaggtg
      421 ctgaagtcgg cagagctttg cgagtttgcc tgctcgctgc ctcagatcac ggccggcgaa
      481 gttctcgaag tctcgcgcgg cgtcgaatgc cgtgtcgagc atttcccggt cggcgtcgtc
      541 gcttcgatca caccgttcaa cttccccaac atggttccga actggacgat cccgaatgca
      601 atcgccctcg gcaactgcat ggtcctgaaa ccgagcgagc tcgttcccat ctcttcgggc
      661 cgcatcgccg agcttctccg cgatgccggt ctgcctgacg gcgtgctcaa catcgttcac
      721 ggtggccagg agacggtcga ggcgatctgc gaccatcctg gaatcgaagg gatcagcttc
      781 gtcggttcga cgaaagtggc aaagatcgtt tatcgccgcg gtacgtcgaa ctacaaacgc
      841 gtcctggccc tcggcggggc aaagaaccac ctcatcgtca tgccggacgc cgatccggag
      901 atgacgtcga gcaacgtcgt ggcctcgatg tcaggctgtg ccggtcagcg ctgcatggcc
      961 gcctcggtga tgatggccgt aggcaagagc gatcacatca tcgagcggat ggtctcgatc
     1021 gctcagtcga tgcagccggg cgttcacatc ggaccggtca tctcggctgc agccaaagcg
     1081 cgcatcgaga gctacatcac agaggccgag aagatgggcg cgaaggtgct cgtcgacggg
     1141 cgcggctaca aggtgaaggg caaagagaac ggctactacg tcggcccgac gttgatcgac
     1201 cgcgtcactc ctgacatgcg gatcgcgcag gaggaagtct tcggaccggt gatggtgatc
     1261 attcgcgcga atgacgtggc cgaggctgtc ggtgtgcagc agcgctcgcc gtatggcaat
     1321 gccgccagcg tcttcaccga aagcggtggc gtggcgcgct acgtcatgga aaacgccagc
     1381 gctggaatgg tcggcgtgaa cgtcggcgtg ccggtgcctc gggagccgtt cagcttcggc
     1441 ggctggaacg attcgaagtt cggcgtcggc gacatcaccg gccgcggctc gatcgagttc
     1501 tggacgaagt cgaagaagat gacgaccaag tggaacaagg aagcaggcgt gaactggatg
     1561 tcgtaacggt gaacgtgaca gggtgatggc cgggctctga atgtgagacg cagcagccca
     1621 tcggaaggtt acgggactgg ttttgtcgag tcgcggactc gcagttccgg ctcgccgatc
     1681 tgcgaggttg ccgggttgcc tcatcgcgat gatgcgagag tcgttgctcg cggcagcgga
     1741 tgactcatcg acgggccagg aagcgggaag cggaggtcac gaagcaatca cgaatcttcc
     1801 agaccggcaa cgtcgacatc cgggcaaccg cgaaccgcat cgctatcttc gtcccgtgag
     1861 tggggagcca aacttctcga atccggactc gagaccgatg agtcggtact cacacctctc
     1921 gagtcggtac tcatgtcatt tgagtccgta ctcatgcccg gcgagtccgt actcacttcc
     1981 ggtgagtccg tactcgcatc gtgtgagtcg gtactcaagt ttggtgagtc cggactcact
     2041 acgtttgagt cggtattcaa gtctcttgag tcggtactca tgttctttga gtccgtactc
     2101 acattgctcg agtccggact caaatccggt gagtcggtac tcatgtttct cgagtccgta
     2161 ctcatatttt ttgagtcggt actcaaattg aaaatcgacc aagtcgccct cggagggtcg
     2221 gaatcacccg attttggtta aaaacggtcc aaaatcgccg aagtactgcc gcaacggacc
     2281 tcgcttattc ccgcgaacgc aatttcatcg ttctctcgat ggcagggatg gccggcgtga
     2341 acgccggtgt gccggtgccg cggcggagcc attcagctgc agcggctgga acgattcgaa
     2401 gttcggcgtc ggcgacatca ccggctgcgg ctcaattgag ttctggacaa agtcgaagaa
     2461 gatgacggcc aagtggaaca aggaagcggg cgtgaactgg atgtcgtgag tagcaagagc
     2521 gcaccggatc attgtcccgt cgtggtaaac tgacaccctt aagtatttct tggtgtgtca
     2581 tgcggctctg gctgttcgtt atcgttcttt tctcgtgcgt cgtcgccaac gctgcgtacg
     2641 tgttcgtgac gccaaacaac ctcgggtcgt ggcacaacgg accgctgttg gtctgcggtg
     2701 cccctcctgg cacagtcgat ttcgtccttg ggccaggtac tcctcccctt ggctcgggca
     2761 gcctccaaat cgatatcccg tcaaactcgc ctctggtcgt cttctttggt tcggcactcg
     2821 acggcgtacc acttaccgat ctcaccgagc ttcgatacat cacgtacgtc gtcatctcgt
     2881 cctcccccgg aatcgcgccg cggcttcttc tgagcatcga taccaataac ccgtccgcac
     2941 cctccgatca gatcgagttc caaccgcgat tacaggacgg ctcgacgccg ggcatcccca
     3001 atcaaggcgc ggtggtcact ggcgcttggc aatcgtggaa cgcactcgca ggtggttgga
     3061 ggtcgctctt cggaccgatc aatgctccgc ttgtaacgct cgccacctat gcagccgcgc
     3121 atcccggcgc ccgtctccaa tccggcaacc tcggacccgg catggccatc ctggccggct
     3181 gtgacggtgc ccgaatgctg gctaacgtcg acgacttcag aatcgccgtc aatggagcgc
     3241 agacgatctt cgatttcgag gtctttccgc ccgtcccggc gttcgagcct tgggtggccg
     3301 cgctgctggc aattgtcctg gtcgtactcg gcttgcacaa gatgtcgtaa cattcccctc
     3361 gttccatgag cgacaacaag acgtggggat cgaccgtgat gggctggttc gtggtcaagg
     3421 accaggccga gggctcggat gagccggtgc cggcggccgc tgacgacgcg gccgctgcgg
     3481 cgccgcaagc ggacgttcct gtcttcagca cgccccctcc gcccgcgccg ggtggcaagg
     3541 tcgacttcga tcaagtcttc gaggccgctg gcatcgatgc cgacgagcgg cagcgcgtct
     3601 cgcgcacgct ggagttgctg acttcacttc cgcccgacac cgaggctgcg gtcaagaagc
     3661 agatcgtgat ggcgtcgctg cgggcgttcg gcgtcccgat cgaaaagatc atcgaagcgg
     3721 gcgcgcagga gttgcaggcg ctcgacggct atatccgtgc tggtgctgca gatacggaca
     3781 aagtgacctc cgaggcggac cagcgcatca aacaatacga agaagaaatc gtaaaactga
     3841 agactgtgat gcaggatcgg gtcgcggaac agcaaagcgt cgtaagctcc tgcaacgaga
     3901 agaagctcga ggtgcagaaa gtcctcgagt tcttcgggca ggaagcggtc gcgcgcgtcg
     3961 ttcgcgaatc gcccaagcta cacgaaccat cagggagctg agaactatgg gagtgttttc
     4021 taggctgggt aatctctgga agggctttct ctctctttgg atcgcggaca tcgagcagga
     4081 ccaccccgag atcgcgtacg agaacgcgat caactcgatg atcgccaagt acgccaagct
     4141 gaagggcgcc accgcggcga tcatccgccg ccgcgaagat ctcgacgatc gtttcaagaa
     4201 ggcgaacgct gatctggccc agacaacggc cgagctgaac acagcggtcg agacgaatca
     4261 ggatgatctt gcggtcatcc tcattcagaa gaagaatcag ctcacggccg acatcgcgga
     4321 gatgcagacg gatctccaga cggctcattc cgatgcggag tcggcgaaga cgtcgctcct
     4381 cggcgtgcag tcagagatca agaagctgca ggccgagcgc gaaacgatgc tggccaagat
     4441 gcagtcggca caggctcgta tcaaaattca ggagcagctc gacggtcttt cggtcgacga
     4501 cgaggtgcgc gcgctcgaca acgtgcggac tcacatcaag aacacgatcg ccgaagcgaa
     4561 cctcggcaaa gagctctccg attcgtcgct cgactcccgt ctggccggcc ttcgcagtca
     4621 ggtcggcgac gttcaggcca aacaggagct cgcagagatg aaggctcgca aagccgcgca
     4681 gcaacagggc cagaagacga tgtagtcatt ggccatcccg ggcctgagcg cgggagctgc
     4741 tcatatcggg ctcgcaagat tttttggagg atctgatgaa actcactccg ttcgcaaaat
     4801 tcttcattac cgtggtggtc cttgccgtcg tcggctatgc ggcctggcac tacaagggcg
     4861 ccgaaattcg tagatgggcc accggcagcg acaaagggca atcccagacc agcacgagcg
     4921 cggtcggcag caacgatttc gatgccctca agaacgcgcc cgccgaccca tcgcgcgatg
     4981 ccgcggtcac cggtgtctcc gctgtcagcg tcaatggcgg caagttgaat cgtccgctcg
     5041 tcgttgccat caacacgtgg gccggtcact cccctgggat cgtcttcaac aacggcttgg
     5101 aaccgaatga tgcgtccaac ttccgcaaga aatatgggct cgacgtgaag ttcgtgttga
     5161 tcgaagatcc ggcctcgaag ctcgctgcat tccgcaaggg cgacgtcgac atcatgtgga
     5221 acaccgtcga caactgggcg cgcgaggctt cgatcctcgc cgagaatggg cagaaggcga
     5281 agtcgatcat catgcaggac tggtcccgcg gcggcgacgg catcgtctcg ctggcgtcga
     5341 tcaactcgat cgagcaactc aaggggcaca ggattgcctg cacccagttc acaccgtcgc
     5401 acttcctgct gctctatctc ctgtcgcagt caggcctgtc ggccgatgat cgggccgccg
     5461 tcgagaagaa catcatcttc acgcaggatg cgccggccgc ggccgcaatg ttcaaagcga
     5521 agcaggtcga tgctgccgta acgtgggagc ccgacctctc cggagccgtc acggcacgcg
     5581 gcgacgaagc gcacgttctg gtctccacta ccgccgccac taacatcatc gccgacacgc
     5641 tttgcgcgcg gcaggatgtg atcgacaagg cccccgaatc ggtgcgtgat ttcgttcacg
     5701 gctggctcga cggcatcgaa atgatgaaga acgatccagc caattcttac gcgatcgttg
     5761 gcaaagcgct gaagctggac accgacacgg tgtcgggaat gctctccgga ctcaagctca
     5821 cgacctatgc ggacaacgcg cagttctacg ggctgtccgg gggcaaagcc cactacgaca
     5881 ctctgttcga gaccgcgttc gtaatctggc gcaagaaggg gctggtcaca cgctcagtcg
     5941 aggcgaagga ctgggtcgac acacgcttcc tcaacgcgct ggccgcgaac tacggaacgc
     6001 agaaggttga agagccgaag attgccgcga aagcgccgtc agcgaacgac cgcgccatca
     6061 tcaacaaaca gatcgaggtt cacttcacgc cggggtccga cgacatcatg ccgggctcgt
     6121 tcttcgtcct cgactccctc ggcgacacga tgacgtcgtt cggaaacacc tacctgcgcg
     6181 tcgaaggaaa caccgacgcc accgggtcac caaccaaaaa catgacgctc tccgaacggc
     6241 gtgctctggc ggtgaagaac tacatcgtca agacgttccc gaacatcgat ccgaaccgtt
     6301 tccagaccat cggccgcggc tcgacgaacc cggtcgccga caactccagc gaagccggac
     6361 gtcagttgaa tcgccgcacg gaaatcaagg tcgttctcac gactcagtaa tgaccgcaca
     6421 tcgcaggaat tcgatctggt cgattcgcca gcccgtgccc cgaggcacgg cgctggcgct
     6481 cgcgttcgtg atgccgatcg tcgtcatcgg cgcgtggagc atcgtcagct acgggcactt
     6541 ggcgcccgcg gactttcttc cgtcgccgag cgaggtgatt cgcggcacgc tacagctctt
     6601 cattcagtac gagctcttca cctcgatcgt aatctcgacg cagcggatcg tgaccgcgtt
     6661 cctcctggcc tctgcggtgg cgattccgct cgggatcctg atgggggcgt ttaacccgat
     6721 caatcacctc ttcgactcga tcgtcgcgcc gttgcggtac atgccgatct cggcattcat
     6781 tccgctgctc attctctggt tcggcattta cgaccaggag aagatcgcct ttctgttcct
     6841 tggggtgttc gtctatctgc ttccggtagt ggtttcggcg atccgtgccg ttcccgagga
     6901 gttggtgcag accgcgctga ccctcggcgc gtccaaatgg caggtcgtcc gcacagtgct
     6961 cctaccggcg gcgcttccag agatcttcga gtcgttccga gtgatgaacg cgatcctctg
     7021 gacctacgtc atcctggccg aggccgtgaa tcccgagcac ggactcggat acatggtcga
     7081 gctggcgcga acgcatcaga aggcttcgtg gtcgttcgcc ggattgatcg tcatcggtgg
     7141 cattggcctc attaccgatc aggtcattcg catgatctct gcgcttctct ttcgctggaa
     7201 ggaagacgct gatggctgac gaacaaccgc aagcaggcga accgggaact gtcgctgtca
     7261 ccgtcgacgc caagtcgtcg aaggcaacct acgtcagcga gcgttccgaa caagcgcggc
     7321 tgcgcatcac tgacctcggc gtgacgtacg tcggccacga tggccgggaa acggaagcgg
     7381 tgcgcgaagt gacgctcgac atcgtcgaca agccgaacgt cggcgagatc gtcgtttttt
     7441 tggggccgtc cggatgcggc aagtcgacga ttctcaaatc ggtcgccggt ctgctcgctc
     7501 cgacgagggg cgagatcctg atcgacggcg tgccggttac cggcgtcggc cgcgatcgcg
     7561 gaatggtgtt ccaggcctac acctcgtttg gatggctgac agtccgccag aacgtcgagt
     7621 acggccttcg gctgcagggt ctcgacgcta aagagcgtgc gacgcgctca aagaagtatc
     7681 tcgaagcagt tggactcgcc gacttcgctg accgttatcc gaaagacctc tctggcggca
     7741 tgaagcagcg cgtggccatc gctcggacgc tgatcaacaa gccgaagctc gttttgatgg
     7801 acgagccgtt cggcgcgctc gatccgcaga cccgttgggg gatgcagagt ctgctcctcg
     7861 acgtatcgcg tgacgaggac aacacgatcc tgttcgtcac gcatgacgta tcggaagcgg
     7921 tctacctcgc cgattcgatc tacgtcatgg ccccgcgtcc gaccaaaatc ctccatcgtg
     7981 tcgacgtgcc ctttttccag aatcgggaca tggctctgaa gtcgtcaaag gagtttcgag
     8041 cagtggaaca gaaactcctc gacttcctgt acgcgccagc ctcgtagaat gatcccgtgc
     8101 ccgatcctcc gaaaaaggac gcctcgccgc tacagaccgt gacgccgcag cgcgcgccag
     8161 ggcaaactgc tgccgcaaag cctgtcgcga agccggcgca accccaggcg gacctcggcc
     8221 gcgccattcg cgagaccgcc accgacgcgg tcaaagaggc tgcctttcat aaccggccct
     8281 acctgaagat ggcattcgcc aatccctaca acctctcgct gctgctcggc ggattgacgg
     8341 cgtccgtgct cacagccaat ccattgcctg gcgtcatcgc tctcggcgcc gaggcgctat
     8401 ggcttctgca cggatccgac tcgcaatacc ttcgccgcat tctctgggac ccgcgcctcg
     8461 aaaagatccg cctggcgatg gatgagcagg accgcaatga gaagatgaag gatcttcccg
     8521 acagcgagaa ggagcgcgtg aatgggctgg tcgcccgcga gcagcagatc aataagctcg
     8581 catctgccaa cccttcattc accggtgacc tgctgcgtat cgagctagtg aagacgcaca
     8641 aactcgtcga gtcgtttctg gagatggccc tgaccgtgaa ccgctatgaa aactacctcg
     8701 gcacggtcga cgtggatgcg ctcgagcgcg atcgggagcg ctggcacgat cgatcgatcg
     8761 caccgcaggc cagtgcgccc gagcgcgaca ttgccctgaa gaactttgcc gtgattcaaa
     8821 agcgcgtcga gcgcgttcac gagatcggcc gatacctcgg cgttgcccgc gcccaactcg
     8881 atctcattga gaactcgttc cagctcatcg ccgatcagat cgtgaccatg caatcgccga
     8941 atgaactgtc cggacaactc gatgatctgc tcgacggtgt cgattccatc cgccagaccg
     9001 caatcgacac agagaaacta ctcaacggaa ccgagagcgt atgacgaaag ccattcttcc
     9061 gaagtgggcc gatgagctgc gcagccgtta cctgcgaggc gaggcctcca tcttcgtcct
     9121 gcacggcaac gtgtatgacg cagtgatcag cgacggccgc atcgttacgc tcaccgagtt
     9181 cctcggcgag gttctgctcg atcagtccaa agagacgatc gcggtttaca acgtggccac
     9241 gggcatccgc tttctcaaac gcgcgcccac ggtcaacatc gacgaggcgc tgctgtccgg
     9301 cgacaagccg cgcgttctgg cggcgctcga gcggatgctc gtgggcggca cgaggacggc
     9361 tgtcatcctc gaatatgcgg aagcgatcgc ccccgccggt gatccgaact ttcaaagtga
     9421 agccgaccgc gctgcgctcg tgacgttgca tcgctggtcg ttcctcgaag agatcgagcg
     9481 aagcgacaac atcgtcatcc tcattgccga gaatttgacc gagctagcga cgaaactcgt
     9541 gtccaatccg cgcgttgctg tggtcgagct cccgatgccc gatcgcgacg agcgtaatgc
     9601 ggcggtccgc gtcgctgaca aacgtcttga tgacaaggat gtcgctcgct acggcgagat
     9661 cacagccggt ctcaagtcca tccagatcat ctccatcctc acgccaccgc ctgcgactga
     9721 ggaggaatcg cgccagcgcg aggacttcat caccagtatc ctcaccggtc ccgacaccgc
     9781 cgaacgcgcc aagaagattg cggcgctgac ggccggtctc agtcacgacg agatcgcgaa
     9841 gttgattgcg cccggtgcgc agcccgccag cattccgcag gatcgcgacg cccgcgcccg
     9901 caaagaagtc gatacgctca tcgcgcaccg caagcgcgag attctcgagc gcgagtgttt
     9961 cggtctgatc gagttcgtgg aaccggcgca cggattcgaa gtcgtcggcg ggatggacga
    10021 ggtcaagaag gacctcctcg tgatcgccaa cagcatccgc aacggcgaga cagcgcgcgt
    10081 tccgatgggt gttctcttca ccggaccgat gggaacgggg aagacgttcg tggccgaggc
    10141 gttcgcgcgc gagtgcggtc tgacgacgat caagctgaag aactttcgct cgaagtgggt
    10201 tggcgcgacg gagggaaatc tagagaagat cctcaacgtc atccgcgcca tcggacaggt
    10261 cgtcgtgatc atcgacgaag gagaccgcgc gttcggcaac acggatggcg aaggcgacgg
    10321 cgggacgtcg tcgcgggtca ttgcccgcat caaggagttc atgtcggaca cgtcgaatcg
    10381 cggacgaatc cttttcctgg tgatgacgaa ccggcccgac aagctggacg tcgatctcaa
    10441 acgcgccggc cgcctcgatc gcaagatccc gttcctctat tcgcagtctg cggaagaggt
    10501 ggaagccgtc gccaatgccc tcctgaagaa gaacgccatc gtcacggcgg tcaacttcat
    10561 cgctctgcgc gagaacttct cgcagaagct catcggctac agcaacgccg acgtggaagc
    10621 agtcatcctg atggcgaatg atgacgctgc gcgcgccggc gcgaccacag tttccgcgga
    10681 caacttcacg catgcggcgt ccgactattt cccaagccgc gactccgaga tgctggagta
    10741 catggagttg cttgccgtct tcgaatcatc gagccggcga ctgctgccgc caaagtacgc
    10801 cgcgatgaca cccgacgagc tcgacctgcg gatgcgcgca ttgcgcgcgg tcgttggctc
    10861 gcggcggtga gctgcaaccg cggttaacgg cgcgcttcga gcttcgcgat gcgagccttg
    10921 tgcatcgcga tctcctgctt gagctcgatg atctcggatt gggatgcgag ttgatcgacc
    10981 tttccttcta catcagcaag gcgggtctca atcgctgaga cacgcgcttc gagtcttgcc
    11041 agagctacct taattagctg caactctgac tcgacgcatc ccagtctgct ctcaacgctc
    11101 tcaagtctgc tctcaacgtt gctcaatctg ctctcaacgt tgccgagtct gctctcaacg
    11161 cgatcaagtc tagcctcgag gcgcgccaat cctgccctga gcgcctgata ctctgtctcc
    11221 agacgatcga gtcggacata tacaccgtcg aacagcgaat tgactcgatc gaaacccgct
    11281 tccatgcgtt ccatgaaggg cgtcatctcg tcgcgcacga ttagtctcag gttctgcagc
    11341 agctcgctcg cttccatcat cacgaatgct acctcggtcc gaatctactg tcacattctc
    11401 tactccatgt agcgtggtat cgttttcgaa ctcagcaaac aggaggggtc catgggcgaa
    11461 cgcgcggtgc gtgaaacggc tcccatccag gtcgagagct ccgaaaatct caacccctat
    11521 gccattgcgc aggcg
//
LOCUS       DATLVW010000959         9935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_453626, whole genome shotgun sequence.
ACCESSION   DATLVW010000959 DATLVW010000000
VERSION     DATLVW010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453626"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="nrfD"
                     /locus_tag="VNN25_28390"
     CDS             complement(<1..856)
                     /gene="nrfD"
                     /locus_tag="VNN25_28390"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXH95529.1"
                     /translation="MKPPRVPDSATEKRLDDIREQAALGRLDAKGVRPSGSPFPEADA
                     EHGYCGLPIVKAPVWTWEVPVYFFVGGAAGASSVLALAAQLSGAPHSLVRDARWIAAI
                     GAAASGPLLILDLGKPERFLNMLRVFKPQSPMSVGAWTLSLFGSASAAAAIGEELGLR
                     RIGDAAAFVAALSGVVMATYTGVLLGATSIPVWAAHARTLPIHFGASALASATAVLEL
                     RGHDARALNVLGLAAAIAETSMGVEIESDKAISSDPLRHGATGTTIRIGGFFSGPLPL
                     ALRLLGIRS"
     gene            complement(853..1686)
                     /locus_tag="VNN25_28395"
     CDS             complement(853..1686)
                     /locus_tag="VNN25_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXH95530.1"
                     /translation="MAVTGFLTDSTLCIGCKACEVACKEWNDVPDDGFVWSGLSYDNT
                     LALGHSTYRHVKFVERQPQAGCGGNSGERMTWEFSSDVCKHCGNAGCLEACPTGSIVR
                     TEFGSVFVQPDICNGCGYCVVSCPFGVIDRRPEDGRAFKCTFCYDRQKAGAEPACAKA
                     CPTGSILFGKLDELEVAADRRIAQLHAAGMNDATIWNPKETSVGGTHAFFIVRGDPRA
                     YNLPSNPEVPTRLLKQSWSAAAIAAGFMIAGTIAAFSLSARNAGVPPAGPAASRRRGA
                     R"
     gene            complement(1724..3865)
                     /locus_tag="VNN25_28400"
     CDS             complement(1724..3865)
                     /locus_tag="VNN25_28400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95531.1"
                     /translation="MDKRVSIVVVLFLLTSLQSARAATEIQLSQPSIGDLALPVRLRT
                     SVASNGRGYIVAWEASSGSIDDVTAIYIRALGADGIPLRPFPTFLGVGREPRAVWNGH
                     EYLLVWGITSPTTGSQFTPSVVGVRLREDGSLIDADPVALVSEANPFSTLTTVVWNGS
                     RYLVTWDRGMALVDADLQHFNLVLMPSTGGNPTYSATSGGSFMVLSQGFSLPQMRLVV
                     IPVSAAGELGTVNLLEGGRGNIIGVDGGYVMLRDDETNLHLSRLRGDGTLLSSSIVAP
                     GRVGYPRLATRDGRIVSSWESLPDETHTRVCVARFDAFTPTLCSTVSAGLQHDPSIGT
                     ASTSILAVWSDTSNGRDNVRGLVAPLSTLPLVSAGAGISVSDASSTPYVERRADATVA
                     VAWSEYNQSSKHFEVHLGGRSSQGEKLAERAVFPNGSDQTVPAIAGGSRRTMALWEEG
                     AGGSSKIRMTIVDDVTKAVVATLPLADGTAPSLSFDGKEWLAAWQSPSGVIRYALIDS
                     DGNAVATGALPGPTGGSFFQTAPAMIWSGKTFFVAWHETVATAPGFQAGESIKMAAID
                     PGGALQVATTLDSADAGLALPSAASSGGRLLVSWGAPAGTLRQMLFDSAGKQLGALIE
                     FASPFAVSRSRTHAMANGFATLAGSRIALTSLDGRALDSFDVPETAAGGDFAVDAANR
                     FILVYSRTLATPSIATFAQTIGLPRRHASDH"
     gene            4020..4601
                     /locus_tag="VNN25_28405"
     CDS             4020..4601
                     /locus_tag="VNN25_28405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95532.1"
                     /translation="MIGFDAAMRGSDAGSNGSDVAMIGSDIGSNGFDAAMIGSDIGSN
                     GFDAAMIGSDIGSNGFDAAMIGFDAGNNGFDAAMIGFDVGSNGFDAAMIEFDAGSNGS
                     DAAMIGFDVGNNGFDAAMIAFDAESDGSGFAIGDGPSEGVRPEVEVTEDGIGEAATIF
                     VTSTLGLTPYTANRGKSSAARSCARCTSALSLP"
     gene            complement(4534..7674)
                     /gene="fdh"
                     /locus_tag="VNN25_28410"
     CDS             complement(4534..7674)
                     /gene="fdh"
                     /locus_tag="VNN25_28410"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7114..7116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HXH95533.1"
                     /translation="MLAELLLKWPLIRQLKTGADGTGVEAMSDATHALRPKNEGTEVA
                     RSICPYCGVGCGQLVYHAEGKVVSIEGDPESPISRGHLCPKGSASYQLVTHDGRATKV
                     KYRAARSREWTELPLDQAMRMVARRMWETRQRTFTETLDGKPVMQCTGMAHLGGATLD
                     NEENYLIKKLFTGGLGMVCISNQARIUHSSTVPGLGTSFGRGGATTAQQDLANADAIL
                     IMGSSMAENHPVGFQWVIEAREKNGATIIHVDPRFNRTSAMADYWLPLRAGSDIVFLG
                     AMINYTLSNERYFREYVVPYTNAATILREDFRDTEDLGGLFSGWDPEKKQYNSKSWLY
                     EGAEKKAAGPDPAMDENRGGHGKDRGGEAQNLHEPHRDPTLQHPRCVFQVLKRHFARY
                     TPEMVEQNCGIPRERFLEVADIFTSASGREKTGVICYAVGWTQHSSGVQIIRAAAILQ
                     LLLGNIGRPGGGIMALRGHASIQGSTDIPTLYDILPGYLPMPMHNAADDNNLAAWIKT
                     HKSPAGWWNNIDKYIVSLLKAYYGDGATKENDFGFEWLPRLTGDHSHFGYWLDMADGK
                     LEGLFVMGQNPAVGAPNARLERKALSKLKWLVVRDLVETETATFWLDSPEISSGELRT
                     EEIETEVFLFPAASHVEKEGTFTNTQRLLQWREQAVEPPGAARSELHFLYHLGRLLKE
                     KATDAPRDAGLRALTWDYPCHGAIQEPSADAVLREIHGWKTDDERSPIKNFSELKADG
                     STASGCWIYSGVYDGENKANGRAPKGKYAHGWGYAWPMDRRILYNRASAAPDGTPWSE
                     RKKLVWWDEAKGEWGGDDIADFTKTKRPDFEGDQKKGGDATLRGDAPFIMHPDGAGWL
                     YVPSGLKDGPLPAHYEPLESNLVNDLYPDQLTNPASHRKERPDNAYATSPDARFPYVL
                     TTYRLTEHHTAGGMSRTLAHLAELQPELFAEISPELAAEVGVINGDHIIVSTARGEIE
                     ARALVTGRMPSLDVQGKRVHQVGLPYHWGGRGLVRGDVVNDLVAISEEPNVRIMEAKA
                     LTCSVRRSEPLTTSLD"
     gene            complement(7677..8201)
                     /locus_tag="VNN25_28415"
     CDS             complement(7677..8201)
                     /locus_tag="VNN25_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95534.1"
                     /translation="MKVISPANRALMTARRDRLRRRGWIALVCAPLGLAPFVITVVLS
                     DRVPTDKQVVVTGLTMFFGILGVIPVCMKLGWDSFAEARRITNFLSDGIAPEPPRLRR
                     PPKWLHPLLLVVALLHNPVAIFRGTERCFGVYAAPWWMVALTLFLFLEAMNLYLWLRS
                     SRLRARGRSFAHKI"
     gene            8225..9010
                     /gene="fdhD"
                     /locus_tag="VNN25_28420"
     CDS             8225..9010
                     /gene="fdhD"
                     /locus_tag="VNN25_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXH95535.1"
                     /translation="MSDTGDATRRVTVRRADGDAYADLVVVEEPLEIRVGDKPVSVTL
                     RTPGHDFDLAVGFLFTESILRSREEIESIRHWGSPNVVRVALREGASVDMQRLKRHFY
                     STSSCGVCGKASIDALRVAIAPIEDEIRVRYELLVSLPDVLRGEQAAFDATGAIHGAA
                     AFARDGSMLYIREDIGRHNAVDKVIGSLFLDGGRADILVVSGRTSFEIVQKCAVARIP
                     ILVAVGAPSSLAIELAHEFGITLLGFVRDGRANVYAGEQRLAN"
     gene            complement(9163..9639)
                     /locus_tag="VNN25_28425"
     CDS             complement(9163..9639)
                     /locus_tag="VNN25_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HXH95536.1"
                     /translation="MTINSEVQALKVHVSLNVRNVEASIDFYRRMLGIEPSKVRTGYA
                     KFDVQNPPVNLALNEVPDLAAPGALSHLGIQVGSTADVLATRLRWERVGLLTRDEMQT
                     SCCYAKQDKTWVRDPDGNEWEVFVVLEDNLPEAAGTSAACCVSKAATSDGAVAASV"
BASE COUNT         1838 a         3059 c         3172 g         1866 t
ORIGIN      
        1 tcgatcggat gccgaggagg cggagtgcga gcggaagagg tccggagaag aagccgccga
       61 tgcggatcgt ggtaccggtc gcgccgtggc gcaacgggtc ggagctgatc gccttgtcag
      121 actcgatctc cacgcccatc gatgtctcgg cgatcgcagc ggcgaggcct aatacgttga
      181 gcgctcgcgc atcgtgaccg cgaagctcga ggactgcggt cgcggacgcc agggctgatg
      241 cgccgaaatg gattggcaat gtgcgcgcgt gcgcggccca taccggaatc gatgtcgcgc
      301 cgagcagcac gccggtatag gtggccatga ccacgccgga gagtgctgcg acgaacgcgg
      361 cagcatcgcc gattcgtctc agaccgagct cttctccgat cgcggcggca gcgctcgcgc
      421 tgccaaagag tgacagcgtc caggctccga ccgacatcgg cgattgcggc ttgaatacgc
      481 ggagcatgtt gaggaagcgc tcaggcttgc cgaggtcgag gatgaggagc ggtccggaag
      541 cggcagcgcc gattgcggcg atccagcgag cgtcgcgcac cagcgagtgc ggcgcgccgc
      601 tcagttgcgc ggcaagcgca aggaccgagg aagcaccggc ggcacctccg acgaagaagt
      661 agacgggaac ttcccacgtc cacaccggcg ctttgacgat cggcaatccg cagtagccat
      721 gttccgcatc cgcctcgggg aacggcgatc cggatgggcg gacgcctttg gcgtcgagac
      781 gtccgagggc ggcttgctcg cggatgtcgt cgaggcgctt ttcggtagcg ctgtccggaa
      841 cacgcggcgg tttcatcgcg cacctcggcg gcgggacgcc gccgggccag ccggcgggac
      901 gccggcgttc cttgcgctaa gtgagaacgc ggcgatggtt ccggcgatca tgaatcctgc
      961 tgcgattgcc gcggcactcc acgattgctt cagcaagcgc gtgggcacct cgggattact
     1021 gggcaggttg taggcgcgcg ggtcgccgcg cacgatgaaa aaggcgtgcg ttccgccgac
     1081 gctcgtctct ttcggattcc agattgtggc gtcgttcatc ccagcggcgt ggagctgggc
     1141 gatgcgacga tcggcggcga cttccagctc atcgagcttg ccgaacaaga tcgatccggt
     1201 cgggcacgct ttcgcgcatg ccggctccgc gcccgctttc tgcctgtcgt agcagaacgt
     1261 gcatttgaat gcgcggccat cttcgggacg ccggtcgatg acgccgaacg gacaggagac
     1321 gacgcagtag ccgcagccgt tgcagatgtc gggctgcacg aagacgctgc cgaattcggt
     1381 gcgcacgatc gaaccggtcg gacacgcttc gaggcagccg gcgttcccgc aatgcttgca
     1441 gacgtcagac gagaactccc acgtcatccg ctcgccagag ttacctccgc atccggcctg
     1501 cggctgccgc tcgacgaact tgacgtgccg gtacgttgaa tggccgagcg cgagcgtgtt
     1561 gtcgtacgaa agcccgctcc aaacgaaacc gtcgtcgggg acgtcgttcc attccttgca
     1621 cgccacttcg cacgctttgc agccgataca aagggtcgag tcggtcagga aaccggtgac
     1681 ggccatgcgc gtcgagctta tcgcattcga aatggccggc gcatcagtgg tcggaagcgt
     1741 gtctgcgcgg caatccaatc gtctgcgcga aggtagcaat tgagggcgtc gccaaggtac
     1801 gcgagtagac gaggatgaat cggttagcgg catcgacggc gaagtctccg ccggcggcgg
     1861 tttccggtac atcgaacgag tcgagagcgc ggccatccag cgaggtcagg gcgatgcggc
     1921 ttcccgcaag cgttgcgaaa ccgtttgcca tcgcgtgggt acgcgagcgc gagacggcaa
     1981 acggcgacgc gaattcgatg agtgcaccga gctgtttgcc ggcggaatca aaaagcatct
     2041 ggcggagcgt cccggccggc gcgccccatg aaaccaggag gcgaccgccg ctggaagccg
     2101 cggacggcaa ggccagcccg gcgtcggcgg agtccagcgt cgtcgctacc tgcaacgctc
     2161 cgccagggtc aatggctgcc atcttgatgc tctcgcctgc ctggaaaccg ggcgccgtcg
     2221 ccaccgtctc gtgccacgcc acgaagaaag tctttccgct ccagatcatc gcgggcgccg
     2281 tttggaaaaa tgatccgccg gtcgggccgg gcaaggcacc cgtggccacc gcgtttccat
     2341 cgctatcgat cagcgcataa cgaatgacgc cggagggtga ttgccacgct gccagccatt
     2401 cctttccgtc gaacgaaaga gacggtgccg tgccgtcggc cagcggcaga gtggcgacga
     2461 cggccttcgt gacatcgtcg acgatcgtca tccggatctt cgacgaacca cctgctcctt
     2521 cctcccacaa tgccattgtg cgtctgctac cgccggcaat tgccggaacg gtctgatcgg
     2581 atccattcgg gaagacggca cgctccgcca gcttttcgcc ctggctgctt ctgccgccga
     2641 ggtggacttc gaaatgcttt gagctctggt tgtactcact ccacgccaca gcgaccgtgg
     2701 catcggcgcg tcgttccacg tacggagtgg acgaagcatc ggagacgctg atccccgcac
     2761 cggccgatac cagcgggagt gtggacaggg gcgcgaccag gccgcggacg ttgtcgcggc
     2821 cattgctcgt gtcgctccat accgcaagaa tcgacgtcga cgccgtaccg atggatgggt
     2881 cgtgttggag ccctgcgctg acggtcgagc aaagagtcgg agtaaacgca tcgaaacgcg
     2941 ctacacaaac acgcgtgtgg gtttcgtcgg ggagcgattc ccacgacgag acgatccggc
     3001 cgtccctcgt tgccagcctc gggtatccga cacgtcccgg cgccacaatg gagctcgaaa
     3061 gcaaggtgcc gtcgccgcgg agccggctca ggtgaagatt cgtctcgtcg tcccgcagca
     3121 tcacgtagcc gccatcgaca ccgatgatgt tcccgcgtcc cccctccagc aaattcacgg
     3181 ttcccaattc tccggccgcg gaaacgggta tgacgacaag cctcatttga gggagcgaga
     3241 agccctgcga aaggaccatg aagctcccgc ccgacgtcgc ggaatacgtg gggttacccc
     3301 cggtcgacgg catcagaacg agattgaaat gctgaaggtc cgcatcgacg agcgccatcc
     3361 cgcgatccca ggtaacgaga taccggctgc cgttccagac aacagtagtg agcgtcgaga
     3421 agggattcgc ctcgctcacc aacgcgaccg ggtcggcatc gatgagtgac ccgtcctcgc
     3481 gtagccgtac gccgactacc gacggggtga attgcgatcc ggttgtgggc gatgtgatgc
     3541 cccacacgag gaggtattcg tgaccgttcc agacggcacg aggctcacgc cccactccga
     3601 gaaacgtagg gaatggccgc agaggaatgc cgtccgcgcc gagggcgcgg atgtagatag
     3661 ccgtgacatc gtcaatcgag cctgaactcg cttcccacgc cactatgtag ccgcgtccgt
     3721 tcgaagcaac agaggtcctc aaccgaacgg gcaacgccaa atcgccgatg ctcggttgcg
     3781 atagctggat ctcggtcgct gcgcgcgcgg actgaaggct ggtcagcaga aagaggacga
     3841 cgacaatcga cactcgcttg tccatgctga aacagcatat tcactaccga acctcgtgtc
     3901 atcatcgaat tccggaactt gcgggcttct cacatgcgca ccaccctttc cggcaaggag
     3961 gacgcaccaa gtgaggcgaa atgcacgcta aaagacggaa agggcggtaa taagtcggaa
     4021 tgattggttt cgatgccgca atgcgcggtt ccgatgccgg aagcaacggt tccgatgtcg
     4081 caatgattgg ttccgatatc ggaagcaacg gtttcgatgc cgcaatgatc ggttccgaca
     4141 tcggaagcaa cggtttcgat gccgcaatga tcggttccga tatcggaagc aacggtttcg
     4201 atgccgcaat gatcggtttc gatgccggaa acaacggttt cgatgccgca atgatcggtt
     4261 tcgatgtcgg aagcaacggt ttcgatgccg caatgatcga attcgatgcc ggaagcaacg
     4321 gttccgatgc cgcaatgatc ggtttcgatg tcggaaacaa cggtttcgat gccgcaatga
     4381 tcgctttcga tgccgaaagc gacggttccg gtttcgcaat cggtgacgga ccttcggaag
     4441 gggtcagacc cgaagtggaa gtgacggaag atggcattgg cgaagccgcg acaattttcg
     4501 tcacttccac tttgggtctg accccttaca cggctaatcg agggaagtcg tcagcggctc
     4561 gctcctgcgc acgctgcacg tcagcgcttt cgcttccatg atgcgcacgt tgggctcttc
     4621 agaaatagcg acgaggtcgt tgacgacgtc gccgcgaacg aggccgcgtc cgccccagtg
     4681 atacggcagg ccgacttggt gtacgcgctt gccttgcaca tcgagtgaag gcatccgccc
     4741 ggtgacgagg gcgcgcgctt cgatctcgcc gcgtgcagtc gagacgatga tgtgatcccc
     4801 gttgatgaca ccgacttccg ccgccagctc gggcgagatc tcggcaaaga gctcgggctg
     4861 cagctcggcg agatgcgcaa gcgtgcgcga catgccgcct gcggtgtgat gctcggtcag
     4921 gcgataggtc gtcagcacgt aggggaagcg cgcgtcgggc gatgtcgcgt aggcgttgtc
     4981 ggggcgctct ttgcgatgcg aggcaggatt cgtgagttga tcgggataga gatcgttgac
     5041 gagattcgat tcgagcggct cgtagtgcgc gggaagcggt ccgtccttca agcctgacgg
     5101 gacgtagagc cagccggcgc catcgggatg catgatgaag ggcgcgtccc cgcgaagcgt
     5161 cgcatcgcca cccttcttct gatcgccttc gaaatcaggg cgcttggtct tcgtgaagtc
     5221 ggcgatgtcg tcgccccccc actccccttt tgcctcatcc caccagacga gtttctttcg
     5281 ctcgctccac ggcgtcccgt cgggtgcggc cgaggcgcgg ttgtaaagga tgcgccggtc
     5341 catcggccag gcgtagcccc atccgtgcgc atacttccct ttcggggctc ggccattcgc
     5401 cttgttctcg ccgtcgtaaa cgcccgagta gatccagcag ccggatgcgg tcgatccgtc
     5461 cgcctttagc tcgctgaagt tcttgatggg agagcgttca tcgtcggtct tccacccgtg
     5521 gatctcgcgc agcaccgcat ccgcgctcgg ctcttggatc gcgccgtggc atgggtagtc
     5581 ccacgtcagc gcacggagtc cggcatcgcg cggcgcatcg gtggctttct ccttcagtag
     5641 ccgcccgagg tgataaagga agtgaagctc gctgcgggcg gcgccgggag gttcgacggc
     5701 ctgttcgcgc cattgcagga gacgttgtgt gttcgtgaac gtcccttctt tctcgacgtg
     5761 cgacgcggcg ggaaagagga agacttcggt ttcgatctct tcggtccgca gctcgccgct
     5821 cgagatctcg ggggagtcga gccagaacgt ggccgtctcc gtctcgacga gatcgcgcac
     5881 gacgagccac ttcagtttcg acaacgcttt tcgctccagc cgcgcgttcg gcgcgccgac
     5941 ggcggggttc tggcccatca cgaacaagcc ttcgagcttg ccgtcggcca tatcgagcca
     6001 gtagccgaag tgcgaatgat cgccggtcag ccgcggcagc cattcgaagc cgaagtcgtt
     6061 ctccttcgtc gcaccatcgc cgtagtaagc cttgagcagc gacacgatgt acttgtcgat
     6121 gttgttccac catcccgccg gcgacttgtg cgtcttgatc cacgcagcga gattgttgtc
     6181 gtcggccgcg ttgtgcatcg gcatcggcaa gtagccgggc aggatgtcgt aaagcgtggg
     6241 gatgtcggtc gatccctgaa tcgatgcgtg gccgcggagc gccatgatgc cgccgccggg
     6301 acggccgata ttgccgagga gaagctgcag gatcgccgcg gcgcgaatga tctgcacgcc
     6361 cgacgagtgc tgcgtccagc cgaccgcgta gcagatgacg ccggtcttct cgcgcccgga
     6421 agcgcttgtg aagatgtccg ctacctcgag gaagcgctcg cgcgggatgc cgcagttctg
     6481 ctcgaccatc tccggcgtgt agcgcgcgaa gtggcgcttc aacacctgga agacgcatcg
     6541 tggatgttgc agcgtcggat cgcgatgagg ctcgtgcagg ttctgcgctt cgccgccgcg
     6601 gtctttgccg tgaccgccgc gattctcgtc catcgcagga tccggccctg ccgccttctt
     6661 ctccgcaccc tcgtagagcc agctcttgga gttgtactgc ttcttctcag gatcccagcc
     6721 gctgaacaac ccgcccagat cctcggtgtc gcggaagtct tcgcgcagaa tcgtggcggc
     6781 gttcgtgtac ggaacgacgt actcgcgaaa gtagcgctcg ttgctgagcg tgtagttgat
     6841 catcgcgccg aggaagacga tgtcgcttcc tgcgcggagt ggcagccagt aatccgccat
     6901 cgccgaggtg cggttgaagc gcggatcgac atggatgatc gtcgcgccgt tcttctcacg
     6961 cgcttcgatc acccactgga acccgaccgg atgattctcg gccatcgacg agcccatgat
     7021 gaggatggca tcggcgttgg cgagatcctg ctgcgcggtc gtcgcgccgc cccgtccgaa
     7081 cgacgtcccc agaccgggga cggtggagct gtgtcatatc cgggcctggt tgctgatgca
     7141 aaccatgccc agacctcctg tgaagagttt cttgatgagg tagttctctt cgttgtcgag
     7201 cgtggcgccg ccgaggtggg ccatgccggt gcattgcatg acgggcttgc cgtccagcgt
     7261 ctcggtgaag gtgcgctgcc gcgtctccca catccgccgc gccaccatgc gcatggcctg
     7321 atcgagcggc agctcggtcc attctcttga tcgcgcagcg cgatacttca ctttcgtggc
     7381 gcggccgtca tgcgtgacga gttgatacga ggcggagcct ttgggacaga gatgaccgcg
     7441 cgagatcggc gactccggat cgccttcgat cgacacgacc ttcccctcgg catggtaaac
     7501 aagttgtcca catccgacac cgcagtaggg acagatggac cgcgcaacct ctgtgccctc
     7561 gttcttcggc cgcagcgcgt gcgtggcatc cgacatcgct tcgacgccag tgccgtccgc
     7621 gcctgttttc aactggcgga tgagcggcca cttcagaagg agctcggcaa gcatcgtcaa
     7681 atcttatgtg caaatgatct tccacgtgct cgcaggcgcg acgagcgcaa ccagaggtag
     7741 agattcatcg cttcgagaaa caagaacagc gtcaacgcaa ccatccacca tggcgccgca
     7801 taaacgccga agcatcgctc ggtgcctcga aagatggcca ccgggttgtg aagaagggcc
     7861 acgaccagca agagcgggtg cagccatttc ggcggccggc gcaggcgcgg tggttcgggc
     7921 gcgatgccgt cgctcaggaa gttcgtgatg cggcgagcct cggcgaacga atcccaaccg
     7981 agcttcatgc aaacaggaat cacaccgagg attccgaaga acatcgtgag gccggtaacg
     8041 accacttgct tgtccgtcgg aactcggtca gagagaacga cggtgatcac aaagggcgcg
     8101 agccccagcg gcgcacaaac cagggcaatc catcctcggc gtctcaaacg gtcgcgcctc
     8161 gccgtcatca acgcacgatt cgccggactg atgaccttca cccgctcatc ttagaatggc
     8221 cgtcgtgtcc gatacaggag atgcaaccag gcgagtcacg gttcgccgcg ccgacgggga
     8281 cgcgtacgcc gacctcgtcg tcgtcgagga gccgctcgag atccgcgtcg gcgacaagcc
     8341 ggtctcggtc acgttgcgga cccctggtca cgatttcgat ctcgcggtcg ggttcctgtt
     8401 cacggagtcg attctgcgca gccgcgaaga gatcgagtcg atccgtcact ggggctcgcc
     8461 gaatgtcgta cgggtcgcgc tgcgtgaggg cgcatcggtg gacatgcagc gcctgaagcg
     8521 gcacttctat tcgacatcgt cgtgtggggt ttgcgggaag gcgtcgatcg atgcgctgcg
     8581 cgtggcgatt gcgccgatcg aggatgagat ccgagtgcgg tacgagctgt tggtctcgct
     8641 gcccgatgtg ttgcgcggcg agcaggccgc attcgatgcc accggcgcga ttcacggcgc
     8701 agcggcgttc gcgcgtgacg gctcgatgct ttatatccgc gaagacatcg gccgccacaa
     8761 cgcggtcgac aaggtgatcg gatcgctctt tctcgacggc ggtcgagcgg atatcctcgt
     8821 cgtcagcggg cggacgtcgt tcgagatcgt gcagaagtgc gcagtggcgc gcatcccgat
     8881 cctggtcgcg gtcggcgccc cctcctccct ggccatcgag cttgcccacg aattcggtat
     8941 cacgctcctc ggcttcgttc gcgatgggcg agcgaatgtc tatgcgggcg agcagcgcct
     9001 ggcaaactga tagagacaag agacaagaga gcagattgcg tactgatctc ttgtctctcg
     9061 atctctgatc tcttgtctct cgatctctga tctctgacta ctctgatctc tgactactct
     9121 gatctctgac tatctccgac ccccgactcc cgactcccgc gctcagaccg atgccgctac
     9181 ggctccatcg ctcgtcgccg ctttcgaaac gcagcacgcc gccgacgttc ccgcagcctc
     9241 gggcagattg tcctcgagca cgacgaacac ttcccactcg ttgccgtcgg gatcgcggac
     9301 ccaggtctta tcctgcttcg cgtagcagca gcttgtctgc atctcgtcgc gcgtcagcag
     9361 cccgacacgc tcccagcgca gccgcgtggc aagaacgtcg gcggtcgagc cgacctggat
     9421 gccgaggtgg gagagggctc cgggcgcggc gaggtccggg acctcgttca gcgcgagatt
     9481 caccggggga ttctgcacgt cgaacttcgc gtagccggtg cggactttgg agggctcgat
     9541 cccgagcatg cggcgataga agtcgatgct cgcttcgaca ttgcggacgt ttagggaaac
     9601 gtgaaccttg agggcctgca cttccgagtt gatggtcatg gtgtctcctt cacatgcgtg
     9661 attacggata tgaagtggaa gataggttcg gaagatacat ttgtcaatgc gaatgtttga
     9721 tggttggaaa ggcgtgtcac gcggaggcgc ggagatgcgg agagaaacac cgcggcctcc
     9781 gcgtcgccgc gtgaaaccgt ctcctgagac aaccgaaact acacgtccgc tttcgtctca
     9841 tacgccgacc cgatccggca aagctgatcg agcgtttcgg cgaagagcag gaagagttga
     9901 tgcaggcgcc actcgtcctt gatcacgccc gtcac
//
LOCUS       DATLVW010000960         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_578750, whole genome shotgun sequence.
ACCESSION   DATLVW010000960 DATLVW010000000
VERSION     DATLVW010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578750"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VNN25_28430"
     CDS             <1..690
                     /locus_tag="VNN25_28430"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXH95537.1"
                     /translation="NFTVVGVMEKRGSSLGNDPDDVVYIPFATAQILYGPDRMTHLPI
                     ALQVKTRDDIDLAKEQVTDILRARHHLKKGQPDDFQITSQEELMKTISGILNTTTGIM
                     GAVVGIALLVGGIGIMNIMLVSVTERTREIGIRKAIGARRYDVLIQFLIEAVTLSGFG
                     GIIGIAGGYGTAALVRLFLKRWFELPPVHTPLWAIVLAFMFCAILGIIFGIYPAAKAS
                     KLDPIEALRYE"
     gene            687..1070
                     /locus_tag="VNN25_28435"
     CDS             687..1070
                     /locus_tag="VNN25_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95538.1"
                     /translation="MSTRMLMASSALVMAALGVTATFLPQEIIDALGGNGRTLPLLVQ
                     VLGATYLGFAMLNWMAKESLIGGIYSRPVSMGNFLHFAVAGIALVKAVAAGERAVATL
                     VCTAIYVVFAVAFGAVVFGRANVAK"
     gene            1552..3291
                     /locus_tag="VNN25_28440"
     CDS             1552..3291
                     /locus_tag="VNN25_28440"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXH95539.1"
                     /translation="MKRLASLIALLIAGAAFAAPKKTTSPMKPITPAELHSLAHDYYE
                     WQKKEYPVGASDQGFHAADDKLDDFSPATTARNAAHVRALLDRVRAADVKGWSKDDTI
                     DWLLFRSQLERDDFPERVQKPEETDPQVYVGEASNAIFSLLKKDYAPGRTRALAATAR
                     LKAIPAMIEQAQRNLKHPVRLYSQLAIDSARSIDPLFSTSLMTIAGDLTPSERESLDA
                     AREEAVAAMHNFADWLEKKLPEMAAWKPMGEANYNYMLRNILLLPMDSRDVAHLGDVE
                     LARYRALEAMLPDPSLADPDPARAKSIPADQQAFLAAYESREQEMIDFLKAKNLVTLP
                     PYLGAFQIRQLPEAFKPTSPGGFMNPPGIYDEDNGGFYFIPTYNPQSKNFYIRAAIED
                     PRPILGHEGIPGHFLQLSIANHLTNEIRRHHGENVFVEGWALYGEEMLMREGLYAPDS
                     SSAGQVLRLSRYRAARIGVDVNLHTGKWTFEQGVNYFMEGGGLDKEAAEGEAAGAASS
                     PTQKISYITGKWQIMRLLGRYRDAKGSAFRLGQFHDDLIANGSLPVSILTWIMLDDPS
                     EIEKATGMKFAAK"
     gene            3316..>3590
                     /locus_tag="VNN25_28445"
     CDS             3316..>3590
                     /locus_tag="VNN25_28445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXH95540.1"
                     /translation="MAIIEKYEPGMFSWIELATSDAAAAKAFYTSIFGWETKDMPIPD
                     GVYTILLKNGNDLGALYQTKEIPPNWATYISVANVDDSAKKATALGAT"
BASE COUNT          739 a         1171 c         1024 g          656 t
ORIGIN      
        1 aacttcaccg tcgtcggcgt gatggagaag cgcggctcgt cgctcggcaa cgatcctgac
       61 gacgtcgtct acatcccgtt cgccacggcg cagatcctct acgggcccga caggatgacg
      121 catctcccga tcgcgttaca ggtgaagacc cgcgacgata tcgatctggc caaagaacag
      181 gtgaccgaca tcctgcgcgc gcgccatcac ttgaaaaaag gccagcccga cgattttcag
      241 atcacctcgc aggaagagtt gatgaagacc atctccggga tcctgaacac gaccacagga
      301 atcatgggcg ccgtcgtcgg catcgcgctg ctcgtcggcg ggatcggcat catgaacatc
      361 atgctcgtat cggtgacgga gcggacgcgg gagatcggga tccgcaaagc gatcggcgcg
      421 cggcgttacg acgtcctcat ccagttcctg atcgaggccg tcacgctctc cggattcggc
      481 gggatcatcg gcatcgccgg cggctacggg accgctgcac tcgtgcgcct tttcctgaag
      541 cgctggttcg agcttccgcc ggtccatacg ccgctctggg caatcgttct cgcgttcatg
      601 ttctgcgcga ttctggggat catcttcggc atctacccgg ccgcgaaagc atcgaagctc
      661 gatccgatcg aagcgcttcg gtacgaatga gcacgaggat gctcatggca tcgagcgcgc
      721 tggtcatggc cgcgctcgga gttaccgcga cgttcctgcc gcaggagatc atcgacgcgc
      781 tcggcggcaa cggccgcacg ctcccgctgc tggtccaggt tctcggcgcc acctacctcg
      841 gattcgccat gctcaactgg atggccaaag agagcctgat cggcggcatc tacagccgcc
      901 cggtatcgat gggcaacttc ctgcacttcg cggtcgctgg catcgcgctg gtgaaggccg
      961 tcgccgccgg cgaacgtgcc gtcgcgaccc tggtgtgcac cgcgatctac gtcgtcttcg
     1021 cagtggcgtt cggagcagtc gtcttcggac gagccaacgt tgcgaagtga atttggcaga
     1081 aaggcctttc acgccgagac gcggagcacg cggagaaagg cttctgcttc cgggttcaac
     1141 tgaagttgag gttatcggac cttaaattga ggttacccga cctgaattta aggttgtcag
     1201 acctcaaatt aaggttactc aaccttaatt taagggttgg tcaaccttaa tttaaagtca
     1261 ggagtcctca aattgaggtt accgaacctg attttgaggt tgcacaacct caatttcaag
     1321 tcgtccaacc ttaatttaag gttgtccaac cacaacttaa ggtcatctaa cctcaattta
     1381 aggtcatcca acctcagttt gaagtcgacg aacccaaatt tgtggtttgc caaactgaat
     1441 gctgcctgcg tcggctacga ggtgacgtcc gacgtccttc aaagagtctt cgcgccttcg
     1501 cgtcttcgcg tgaccggctt tgaaaacgcg accgcgctac actccgcctt catgaaacgt
     1561 ttggcctctc tcatcgcact actgatcgcc ggtgccgcat tcgccgcgcc gaagaaaacc
     1621 acctcgccca tgaaaccaat cacccccgcc gagctccatt cgctcgctca cgactactac
     1681 gaatggcaga agaaggagta cccggtcggc gccagcgacc agggctttca tgcggccgac
     1741 gacaaactgg atgacttctc gcccgctacg accgctcgca atgccgcaca cgtccgggca
     1801 ctgctcgatc gcgtacgcgc ggccgacgtc aaaggctggt cgaaggacga caccatcgac
     1861 tggctcctct tccgctcgca gctcgagcgc gacgacttcc ccgagcgcgt gcagaagccg
     1921 gaggagaccg acccgcaggt ctacgtcggc gaggccagca acgccatctt ctcgctgctg
     1981 aagaaggact acgcgcccgg ccgcactcgc gcgctcgcgg ccaccgcgcg gctcaaagcc
     2041 attccggcca tgatcgagca ggcccagcgc aatctcaaac atcccgtccg cctctactcg
     2101 caactggcca tcgactctgc gcgctccatc gatccgctct tctccacgag cctgatgacg
     2161 atcgccggcg atctgacgcc ttccgaacgg gagtcgctcg atgccgcccg cgaagaagcg
     2221 gtcgccgcga tgcacaactt cgccgactgg ctcgaaaaga agctccccga gatggcggcc
     2281 tggaagccga tgggggaggc caactacaac tacatgctgc gcaacatcct ccttcttccg
     2341 atggattcgc gcgacgtcgc gcacctcggc gacgtggagc tggcgcgcta tcgtgccctc
     2401 gaggctatgt tgcccgatcc gtcgctggcc gatcctgatc ccgcgcgcgc caagagcatt
     2461 cccgcggatc agcaggcctt tctcgccgcg tacgagagcc gcgagcagga gatgatcgac
     2521 ttcctcaagg ctaagaacct ggtcacgctc ccgccttacc tcggcgcatt ccagatccgc
     2581 cagcttccgg aagcgttcaa gccgaccagc ccgggaggat tcatgaaccc gccgggcatc
     2641 tacgacgagg acaacggcgg cttctacttc atccccacct acaacccgca gagcaagaac
     2701 ttctacatcc gcgccgccat cgaagacccg cgccccatcc tcggacacga aggcattccc
     2761 ggccacttcc tgcaactctc gatcgccaat cacctcacca acgagatccg ccggcatcac
     2821 ggtgagaacg tcttcgtcga aggttgggcg ctgtacggcg aggagatgct catgcgcgaa
     2881 ggtctctacg cgcccgactc gtcgtcggcg gggcaggtgc tgcgcctttc gcgctatcgc
     2941 gccgcgcgca tcggcgtcga cgtcaacctg cacaccggca aatggacctt cgaacagggc
     3001 gtgaactact tcatggaagg cggcgggctc gataaggaag ccgcggaagg agaggccgcc
     3061 ggcgcggcat catcgcccac gcagaagatc agttacatca ccgggaagtg gcagatcatg
     3121 cgccttctcg gccgctaccg cgatgccaaa ggcagcgcgt tccggctggg acagtttcac
     3181 gatgatctga tcgcgaacgg atcgctgccc gtatcgatcc tgacgtggat catgctcgac
     3241 gatccgagcg agatcgagaa agcgaccggc atgaagttcg ccgcgaagta gaattcgcgc
     3301 cgcggaggaa tagcgatggc catcattgaa aaatacgagc ccgggatgtt cagctggatc
     3361 gagctggcca caagcgacgc cgcggcggcg aaggccttct atacctcgat cttcgggtgg
     3421 gagacgaagg acatgccgat cccggacggc gtctacacga tcctgctgaa gaacggcaac
     3481 gacctcggcg cgctgtacca gaccaaggag attccaccga actgggcgac gtacatctcc
     3541 gtcgcgaacg tcgacgattc ggcgaagaag gcgaccgcgc tcggcgccac
//
LOCUS       DATLVW010000961         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_454357, whole genome shotgun sequence.
ACCESSION   DATLVW010000961 DATLVW010000000
VERSION     DATLVW010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454357"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..517
                     /locus_tag="VNN25_28450"
     CDS             212..517
                     /locus_tag="VNN25_28450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95541.1"
                     /translation="MADSRQFLELASGASGILTKLASIPSGADNAIIRVDAGTVRLSE
                     SSGSWLNASIGIMFSTGDQAFRIGNGIPLTGFAFVPLGGAAAIQISYYSYHGIGQYL"
     gene            607..801
                     /locus_tag="VNN25_28455"
     CDS             607..801
                     /locus_tag="VNN25_28455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95542.1"
                     /translation="MPGLFSKDELAVVERLTLGKRLTEEDKELLPGLREKLGAGTQAD
                     IADQFLLLTGWTNKPTEYPA"
     gene            959..1720
                     /locus_tag="VNN25_28460"
     CDS             959..1720
                     /locus_tag="VNN25_28460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95543.1"
                     /translation="METASPRAEHWTPRVPLTAAERRVDFPAHNAHIIQRRAAVEHAA
                     APAARRLAHLVAEHGLHTSQVQQELEARLYVSLTRSVDFGYAQVQRELRGVRPTIRAA
                     LRIPGIGDRGRTVLQGLPGIHGLVRSRATLVASNVADEARAAKHDAALNPAATPVTVR
                     LAVGDAVRKALHNDVLQLVGEALNLGRAAGAMETPNPPEYAMRSEQLDENTCDSCDEL
                     HGLIVQVDSAEYLDEMPPAECEGGGRCRGIYVYGD"
     gene            1721..>2476
                     /locus_tag="VNN25_28465"
     CDS             1721..>2476
                     /locus_tag="VNN25_28465"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HXH95544.1"
                     /translation="MPTIHEQQPKLEKLKRELEAGGEMSAVGVFTAAATEVGGDGTSW
                     IEVMPAVTEAKNGPYFFTITKEDLGLLVADIEAHPDKISIDYDHAGAEKGDTRAAGWY
                     TGEAQVVLAGEKTPRGDTAEQDSVWAKVKWTPKAVQEIRDGEFRFVSAEWSMETKDSK
                     SGLWTKFEELAASTLTNRPFFKDLAPVTAKEYASAVAELVVAVEDDDTVHLREAVDAF
                     LAADNAPYGDVEYADPGYQKDGQKRYPLDTETHI"
BASE COUNT          494 a          782 c          784 g          416 t
ORIGIN      
        1 agaagatcga gaagttgcag gcgcgcgtcg ctgaacttga agcgaccgcc ccggctcctg
       61 tcggtgacgc tgaccgcgtg ttcggtcctg acgctgtccc gttggagcag cggtaatgcc
      121 accgcccaag cctcctcgcc cggatgctgg tgggccggca tcaccgatcg acacgatcaa
      181 accgatcggc ccgatcccta tggaggacta gatggccgac agcagacaat ttctcgaact
      241 cgcctccggc gcgagcggca tcctgacgaa gctcgcctcg atcccgtccg gtgccgacaa
      301 cgcgatcatc cgtgtcgatg ctggcacggt gcgcttgtcc gaatcgagcg ggtcttggtt
      361 gaacgcttcg atcgggatca tgttctcaac tggcgaccag gcgttccgga tcggcaacgg
      421 gattccgctg accgggttcg cgttcgtgcc cctcggcgga gccgccgcaa tccaaatctc
      481 gtactacagc taccacggca tcggccagta cctctaagga ggagccatga ctttcacctt
      541 cggcgacgtg tgcctcgcca tcatcgcggt gtgctccgtc atcctcaccg tccacttcgt
      601 cttctaatgc cgggcctgtt cagcaaggac gaactggcgg tcgtcgagcg gttgacgctc
      661 ggcaaacggc tgaccgagga agacaaagaa ctgttgccgg gacttcgcga gaagctcggg
      721 gcaggcacgc aggctgacat cgccgaccag ttcctgctgt tgaccggctg gacgaataag
      781 ccgacggaat acccggcatg agcgcgcagg cagcgccggc gaagcgtacg gccacgaccg
      841 aactctcgcg gaaattcatc cagatctcgt ctgggcagcc attcggtcaa ggactgaccc
      901 tgttcgcact agacgaagcc ggtgacgtct ggcagatcat ccccggaagg gaagaccagt
      961 ggaaactgct tccccgcgtg cggagcactg gacaccccgc gtcccgctga ccgcggccga
     1021 gcggcgcgtc gacttcccgg cccacaacgc gcacatcatc cagcggcgcg cggccgtcga
     1081 gcacgccgcc gcacccgccg cccgtaggct cgcccacctc gtagccgaac acggacttca
     1141 cacctcccaa gtgcaacagg agctggaggc gcgcctctat gtatcgctta cccggtcagt
     1201 cgacttcggc tacgcacagg ttcagcgtga acttcgagga gtccggccga cgatcagggc
     1261 ggcgttacgt atccctggta tcggcgaccg cgggcgaacc gttctccaag ggctgccggg
     1321 tattcacggg ctggtcagga gtcgggccac ccttgtggcg tcgaacgtcg ccgatgaggc
     1381 tcgcgctgcg aagcacgacg ccgcgctgaa cccagccgcg acgccagtca ccgtccgtct
     1441 agccgtcggg gacgctgttc gcaaggcact ccataacgat gtgttgcagc ttgtcggtga
     1501 ggcgctcaac cttggccggg ccgccggagc gatggagacg ccgaacccgc ctgagtatgc
     1561 gatgcggagc gagcagctcg acgagaacac ctgcgacagc tgtgacgagt tgcacggcct
     1621 gatcgtccaa gtcgattccg ccgaatatct cgacgagatg ccaccggccg agtgtgaggg
     1681 cggaggacgc tgccgcggaa tctacgtcta cggagactga atgccaacca tccatgagca
     1741 gcagcccaag ctcgagaagc tgaaacgcga gctcgaggca ggaggtgaga tgagcgcagt
     1801 aggagttttc actgccgcgg ccaccgaggt gggcggcgac ggaacctcat ggatcgaggt
     1861 gatgccagcc gtcaccgaag ccaagaacgg cccctacttc ttcacgatca cgaaggagga
     1921 tctcggtctc ctcgtcgcgg acatcgaggc gcaccccgac aaaatctcga tcgactacga
     1981 ccacgcagga gccgaaaagg gcgacacgcg cgccgcgggt tggtacaccg gcgaggccca
     2041 ggttgtcctg gctggtgaga agacgccgcg cggcgatacg gccgagcaag actcggtgtg
     2101 ggcgaaagtg aagtggacgc ccaaggcggt tcaggagatc cgtgacggag agtttcgttt
     2161 cgtcagcgct gagtggtcga tggagaccaa ggattcgaag tcgggtctgt ggacgaagtt
     2221 cgaggagctc gccgcgtcca cactcacgaa tcgtccgttc ttcaaggatc tcgctcccgt
     2281 cacggccaag gagtatgcat ctgcagtcgc cgagctggtg gttgctgtcg aggacgatga
     2341 cacagtgcat ctcagagaag cggtcgatgc ttttctggct gcagacaatg cgccgtacgg
     2401 cgatgtcgaa tacgccgacc ctggctacca gaaggacggc cagaagcggt atccgctcga
     2461 cacggaaacg catatt
//
LOCUS       DATLVW010000962        10788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_495705, whole genome shotgun sequence.
ACCESSION   DATLVW010000962 DATLVW010000000
VERSION     DATLVW010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495705"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VNN25_28470"
     CDS             <1..285
                     /locus_tag="VNN25_28470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95545.1"
                     /translation="AASCTPDEMFCTRDAAVCTHDAAVCTHYATACTDDAAACTHDAT
                     ACTHDAAGCTHDAAFCTGDAAVSAPDAADCTHEGAFHTRHAVFRTIDRDS"
     gene            455..814
                     /locus_tag="VNN25_28475"
     CDS             455..814
                     /locus_tag="VNN25_28475"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HXH95546.1"
                     /translation="MRTLLHAVIQNAIVTSAATLWPVSLRVDAFVLHAAEILPFEEVE
                     VVNTSTGERFRTWIEPAQSGSGEVALHAGTRSPVRSGDTISILSFATFHEGQTIAHKP
                     RIVTLDANNHVVSVGEG"
     gene            811..1851
                     /locus_tag="VNN25_28480"
     CDS             811..1851
                     /locus_tag="VNN25_28480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95547.1"
                     /translation="MKSRSLIPRVVCALLFLSLAATTLPRLVARTREALTLLPMPYES
                     RRERQMGAWYASVQSLRKTLPRKEPVALVLGIRDTEAAIFANYYLYPIRTRLFAGRND
                     YRDAAPDPTRPKTIVAVTSEKVEKTSYEILRDRDLRAGHRVVVAPEMSEPATSFVLPI
                     AASLDGAAPETFVIEATLADQTGPALSNASMERLPRTNEVRITFWPKGESRTLRIGAG
                     DTVSYYDLVYQLFGVMDSGWIQVESRLPLRAAFYFVNRGRADATRLPNVQRFATRIRP
                     SPLHRDAKLFLLNWGDSIAVTTVGAETIPIMPHAMVTKPISTIPAVSGNVYAFVTTRE
                     LNARTNFYWPEP"
     gene            1859..3163
                     /locus_tag="VNN25_28485"
     CDS             1859..3163
                     /locus_tag="VNN25_28485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95548.1"
                     /translation="MIGLLITLVLFPLVGWSVGRRSWGEAFLFGAGLVGTILFLAGVV
                     HVPLVPAFALVLLTSLGTAVWSYRTRKTSCPTPRPTHHTLLPTIAMALPLIAITFIAS
                     ITPLNDFDGRAFWVLKAKGIAHEHAIDGPFFQGATLDPRNQYPILIPIDGAVIFALAH
                     DLDDRQLRWLYVGLLAALALLVRERISNLVSPAAGAWCAALLVWIPQFAVATEGGALS
                     AYNDIAIAAFAAGAFFELVAEVLPPRDSDIVQPRYREIRFGLWLVFLILTKSEGLPFA
                     LVFLLIGAFAFRKRIATPAILAAIAAVARLVWRARIPAGDEENFVRMLPTIPEKLDNL
                     RAALLAFPRHMAALSSWGAFWIAVIIAAACAIRVNRRPATIAIAVIASMMAVYCGVYV
                     ATEWVVTDLIAVTANRLLMHLAAPALFLLAFAAQWWTRTKSM"
     gene            complement(3133..4620)
                     /locus_tag="VNN25_28490"
     CDS             complement(3133..4620)
                     /locus_tag="VNN25_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXH95549.1"
                     /translation="MTDEHTHNGCNHSSMLQDVARQAMIDKGLAPDFSPEAEKQAETV
                     TGPAQDNDASIKDLRDLLWCSIDNDDSRDLDQLTVAEDLGGGNVRAMIAVADVDAVVK
                     KGSPIDDHARQNTTSVYTAARIFPMLPEKLSTNLTSLNENEERIALVMDIVVDSTGSV
                     TKGDVYRARVHNKAQLAYNALGMWLEGKGELPPKAKAVPGLAENLKLQDKLADTMREL
                     RHEHGALDLETIEARAVMSNGQVVDLQVEARNNAKNLIEDFMIAANGVSARYLAAKGF
                     PSLRRVVRSPERWDRIEAIAAQYGEKLPGEADAQALEEFLVRRRKADPLRFPDLSLAI
                     VKAMGAGEYVVEMPGQTPIGHFGLAVKDYTHSTAPNRRFPDLITHRLIKAALAGQTSP
                     YTRDELEALATHCTEAEDAANKVERQVRKSAAACILEPRIGDRFDGLVTGASEKGTWV
                     RVFHPPVEGKVVHGAQGLDVGDKVTVKLIDVNVERGYIDFVRVHH"
     gene            4651..6105
                     /locus_tag="VNN25_28495"
     CDS             4651..6105
                     /locus_tag="VNN25_28495"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXH95550.1"
                     /translation="MTPKVTRAGDRGLLADFGPDVAALELHARAAALRARDDVIACIV
                     GHQSLYVIFRGVPELDFDDVPPITVTPRVHVIDVDFSGPDLDELLAHTRVTRDAFLAR
                     VPSLRLTARYLGFRAGFAYLEGWPEEWRMPRRPRSRNLVPRGSFAIAGAMAGFYPVDS
                     PGGWNILGRTNATLWDPNVEPPNRFAPGDVVQLRAVPIDRFDAPPGDRVVASGEVVAE
                     VVMPGQLTTIVGARDWKRAAYGVSPGGAFDAMAAASANRAAGNGDVPLLECVLVAPRL
                     RFRTARVAAFCDGSGLVRTFTLSAGEALDIGRFHGGLHGYLAIEGGIDEMRASFAEGP
                     HVLRKGDELRVAGGGSSAVAQSFDGRRDPHLIRVIAGPHEAPPLPEEWEVTSEMNRIG
                     IRLRRRAGGGTAGGPPALHQTDARHDLRELPSCGMQFGTLQWHPDGSLVAMGPDHPVT
                     GGYLQPATVISEDLWKLAQLAPGERIRFEVVGEA"
     gene            complement(6188..7477)
                     /gene="rho"
                     /locus_tag="VNN25_28500"
     CDS             complement(6188..7477)
                     /gene="rho"
                     /locus_tag="VNN25_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXH95551.1"
                     /translation="MLNKRSDPTLAAETLDIRVLKEMKLPELTKVAKEHGIENATGMR
                     KQDLIFSVLQVQTEKSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRR
                     FDLHTGDTVSGQVRPPKEGERYFALIKVEAVNFEHPDVAREKILFDNLTPLYPEQRIK
                     LEVPSNMSSRVLDLIAPVGKGQRGLIVAPPRTGKTMILQAIANSITSNHPEVTLIVLL
                     IDERPEEVTDMQRSVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHRRDVVILLDS
                     ITRLARAYNTVVPPSGKVLSGGVDSNALQRPKRFFGSARNVEEGGSLTIIATALIDTG
                     SRMDDVIFEEFKGTGNMEVHLDRKLSDKRVFPAIDINKSGTRKEELLLERNELNRIWV
                     LRKVFSPLSTVESMELLVEKLEKTRTNSEFLNAMSSM"
     gene            complement(7803..8381)
                     /gene="yihA"
                     /locus_tag="VNN25_28505"
     CDS             complement(7803..8381)
                     /gene="yihA"
                     /locus_tag="VNN25_28505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXH95552.1"
                     /translation="MDIRTVEFSRAAYKADDFPTDRRPQFAMVGRSNVGKSSVINAVL
                     KRKGIARVSQTPGKTQAIQFYLINERFYIVDFPGYGYAKVPRDIARSWGELVRGYLES
                     ADALKLIFILLDVRRTPSPEDRQMHDWTVASGIDERILLTKTDKLSNNQLGKSRAAIA
                     KELEITEDRLITSSVITKTGIEQVRREIASRL"
     gene            complement(8629..9009)
                     /locus_tag="VNN25_28510"
     CDS             complement(8629..9009)
                     /locus_tag="VNN25_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXH95553.1"
                     /translation="MRAVDTNVLVRLITGDDERQAVVAEAFVNRGVWISHVVLVETAW
                     VLRRAYKLDDAGIAAAIEMLLNQEAVSLQDADVVTSALDQYRLRPSLGFSDCLVIEIA
                     RKAGHLPLGTFDKNLAKLDGAERL"
     gene            complement(8996..9256)
                     /locus_tag="VNN25_28515"
     CDS             complement(8996..9256)
                     /locus_tag="VNN25_28515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXH95554.1"
                     /translation="MALPQSKVTAQGQISVPAEIRKELGIGPGSVLEWHHHGDDIVVR
                     KAGKYSFEDIRRALFPTRPRARSLEELKQAIGDHLLEKHARR"
     gene            complement(9328..>10788)
                     /gene="lon"
                     /locus_tag="VNN25_28520"
     CDS             complement(9328..>10788)
                     /gene="lon"
                     /locus_tag="VNN25_28520"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXH95555.1"
                     /translation="EIKDIDLAESILNEDHYGLDKIKERILEFLAVRQLIKGPKGTIL
                     CFVGPPGVGKTSLAKSIARATGRKFVRLSLGGVRDEAEIRGHRRTYIGAFPGQIIQRM
                     KKAGTINPVFLLDEVDKMSMDFRGDPSAALLEVLDPEQNNAFTDHYLDVEYDLSKVMF
                     IATANVTHTIPAPLKDRMEIISLSGYTHLEKLAIGRQFLVPKQRQEQGIEESQVDFLD
                     TAIEELVDSYTREAGVRSLEREIGSVLRKIARQVVKKGPEIKTIMDRSKVRELLGKPK
                     FRSQTIHDKSEIGLATGLAWTEVGGEILSTEVAISRGKGNLTLTGKLGDVMQESARAA
                     LSYVRSRAELFGLDADFHASLDIHIHVPEGAIPKDGPSAGITMATALLSAVTRIPVHR
                     DIAMTGEITLRGKVLPVGGVKDKILAAARAGITRIILPAENERDLEDIPADVREKMEF
                     NLVESMDEVVLHALDGTIVPLVKKLPDAVVSEPPIH"
BASE COUNT         1819 a         3300 c         3419 g         2250 t
ORIGIN      
        1 gcggcgtcct gcacccccga tgagatgttc tgcacccgcg atgctgcggt ctgcacccac
       61 gatgctgcgg tctgcaccca ctatgctacg gcctgcaccg acgatgctgc ggcctgcacc
      121 cacgatgcta cggcctgcac ccacgatgct gcgggctgca cccacgatgc tgcgttctgt
      181 accggcgatg ctgctgtttc cgcccccgac gctgcggact gcacccacga aggcgcgttt
      241 cacacgcgcc atgccgtgtt tcgaaccatc gatcgtgact cttgaaccgc cactggtgtg
      301 ttttgggtag tggtctggtt tgaaacctgg gccgtagccg gttgtcgcgt cgttctgctt
      361 ccctcgacaa cgcggcgcgc cagcgccgca atcaaccgac aacggacaac gcactcggca
      421 ccgccgagtg caactagcta gtacgattac attgatgcgc acgcttctcc atgcggtcat
      481 tcagaacgcg atcgtcacca gcgcggctac gctctggccg gtctctctgc gcgtcgatgc
      541 cttcgttctg cacgccgcgg agatcctgcc gttcgaggag gtcgaggtcg tcaacaccag
      601 caccggcgag cgatttcgca cgtggatcga gccggcgcag tcgggaagcg gcgaggtcgc
      661 gctgcatgcg ggaacgcgct cgccggtgcg gagcggcgac acgatcagca tcctctcctt
      721 tgccacgttt catgagggac agaccatcgc gcacaagccg cgcatcgtga ctctggacgc
      781 gaacaatcat gtcgtgtcgg tcggcgaagg atgaaaagcc gctcgttgat tccgcgcgtc
      841 gtttgcgcgc tgctgttcct gtcactcgca gcgacgacct tgccacgatt ggtggcacgc
      901 acgcgcgaag ctttgacgct gttgcctatg ccgtacgagt cgcgaaggga gcggcagatg
      961 ggagcgtggt acgcatcggt tcagtcgctg cgtaaaacgc ttccaaggaa ggagccggtc
     1021 gcgctggtcc ttggtattcg cgatactgaa gcggccattt tcgccaacta ctacctgtac
     1081 ccgatccgca ccagactctt tgcaggacgc aacgactatc gagacgccgc tcccgatcct
     1141 acgcggccga agacgattgt cgcggtgacg tcagagaaag tggagaagac gtcgtacgaa
     1201 atcctccgcg accgcgattt gcgcgcggga caccgcgtcg tcgtcgcgcc agagatgagc
     1261 gagccggcaa cttcattcgt tcttcctatc gcggcctcac tggatggtgc tgcgccggag
     1321 acgttcgtca tcgaggcaac gctcgccgat cagaccggac ccgcgctctc taacgcgtcg
     1381 atggaacggc ttcctcgcac gaacgaggtt cgcatcacgt tctggccaaa aggagagagc
     1441 agaacactga ggattggggc cggagatacc gtctcgtact acgacctcgt ctatcaactc
     1501 ttcggcgtca tggatagcgg ctggatacag gttgagtcac ggcttccgct gcgcgccgcg
     1561 ttctatttcg tgaaccgagg acgtgccgat gcgacgaggc tgccaaacgt gcagcggttc
     1621 gccacgcgca ttcgtccgtc gccgcttcat cgcgacgcca aactttttct tctcaattgg
     1681 ggcgattcga tcgcggtgac caccgtcggc gccgagacaa tcccgatcat gccgcacgcg
     1741 atggtgacga agccgatcag cacgattccg gccgtcagcg gaaacgtgta tgcgttcgtg
     1801 acgacccgtg agctgaacgc caggactaac ttctactggc cggaaccgtg acctcgacat
     1861 gatcggtctg ctcatcacgc tcgtcctttt cccgctcgtt ggctggagtg ttgggcgacg
     1921 aagctggggc gaagcgttcc tcttcggcgc gggtctcgtc gggacgatcc tcttcctcgc
     1981 cggcgtcgtg cacgtgccat tggttccggc gttcgcgctg gtgctgctta caagcctcgg
     2041 cactgcagtg tggagctatc gaacccgcaa gacgagctgc cccaccccac gccccacgca
     2101 ccacacccta ctccccacga tcgcgatggc cttaccgctg atcgcgatca ccttcatcgc
     2161 ctccatcacg cccctcaacg acttcgatgg ccgcgcgttc tgggtgctga aggcgaaggg
     2221 gatcgcgcat gagcacgcaa tcgacgggcc gttctttcag ggcgccacac tcgatccgcg
     2281 caatcaatac cctattctga ttccgatcga cggtgccgtg atcttcgccc tcgcccacga
     2341 tctcgacgac cgtcaacttc gctggctcta cgtcgggctc ctcgccgcgc tcgcgctgct
     2401 agtgcgcgag cggatctcga atctcgtctc gcccgcagcg ggcgcgtggt gcgcggctct
     2461 gcttgtctgg attccgcagt ttgccgttgc cacggaagga ggtgcgcttt cggcgtacaa
     2521 cgacatcgct atcgccgcgt tcgccgcagg cgcatttttc gagttagtcg cggaggtctt
     2581 accccctcgc gacagcgaca ttgtccaacc acgctaccgc gagatccgtt tcggcctctg
     2641 gctggtcttc ctcatcctca ccaagagcga agggctgccc ttcgcgctcg ttttcctcct
     2701 cattggcgca ttcgcctttc gcaaacggat cgctacgcct gcaatcctcg cggcgatcgc
     2761 tgccgtcgcc cgtctggtgt ggcgtgctcg catcccggca ggcgatgaag agaatttcgt
     2821 tcgcatgctg ccgacgattc cggagaagtt ggacaacttg cgcgccgcgc tgctcgcatt
     2881 tccgcgtcac atggccgccc tctcgagctg gggcgccttc tggatcgcag tcatcatcgc
     2941 ggcggcatgc gcgatccgtg tcaatcgccg tcctgccacg atcgcgatcg ccgtcatcgc
     3001 gtcgatgatg gctgtctact gcggcgtcta cgtcgccacg gaatgggtcg tcacggatct
     3061 gatcgccgtc acggccaacc ggctactgat gcatctcgcc gcgccggcgc tcttccttct
     3121 cgccttcgcc gctcagtggt ggacgcggac gaagtcgatg tagccgcgct caacgttcac
     3181 gtcgatcagc ttgacggtta ctttgtcgcc gacgtcgagc ccctgcgcgc cgtgcacgac
     3241 cttcccttcg accggcggat gaaagacgcg cacccacgtc cctttttcgg atgcgccggt
     3301 gacgagtcca tcgaagcggt ctccgatgcg cggctcgaga atgcatgcgg cagcggactt
     3361 gcgaacctgc cgctccactt tattcgccgc atcctccgcc tcggtgcagt gcgtcgccaa
     3421 cgcctccagc tcatcgcgcg tgtacggcga cgtctggccc gccagggcgg ccttgatgag
     3481 gcgatgcgtg atgagatcgg gaaagcggcg gttcggcgcc gtcgagtgtg tgtaatcctt
     3541 cacggccaat ccaaaatggc cgatcggtgt ctggccgggc atttcgacga cgtactcacc
     3601 ggctcccatc gccttcacga tcgccagaga gagatcgggg aaacggagcg gatcagcttt
     3661 gcgacggcgg acgagaaact cttcgagagc ctgcgcgtcg gcctcgccgg gcagcttctc
     3721 gccgtactgc gcggcgatgg cttcgatgcg atcccagcgt tccggtgacc gcaccacgcg
     3781 ccgcaggctg gggaaccctt tcgccgcaag gtaacgtgcg ctgacgccgt tcgccgcgat
     3841 catgaagtcc tcgatcaggt tcttggcgtt gttacgcgcc tcgacctgca gatcgacgac
     3901 ttggccgttg ctcatgaccg cgcgcgcctc gatggtctcg agatcgagcg ctccatgctc
     3961 gtgccgcaat tcgcgcatcg tgtcggcaag cttgtcctga agcttcagat tctccgcgag
     4021 gcccggcacc gctttggcct tcggcggcag ttcgcccttc ccttccagcc acatgccgag
     4081 cgcgttgtag gcgagctgcg ctttgttgtg aacgcgggcg cgatagacat cgcccttcgt
     4141 caccgagccg gtggaatcga cgacgatgtc catgaccagc gcgatccgct cttcgttctc
     4201 attcagagac gtcaggttcg tcgacaactt ctcgggcagc atcggaaaaa tgcgtgctgc
     4261 ggtgtagacc gacgtcgtgt tctggcgagc gtgatcgtcg atcggcgagc ctttcttgac
     4321 gacggcatcg acatcggcga cggcgatcat cgcgcggacg tttccaccgc cgaggtcttc
     4381 ggcgacggtg agctggtcga ggtcgcggga gtcgtcgttg tcgatcgagc accagaggag
     4441 atcgcggaga tctttgatcg acgcgtcgtt atcctgcgcc ggtcccgtca ccgtctccgc
     4501 ctgcttctca gcttcaggcg agaagtcggg agcgagacct ttgtcgatca tcgcctggcg
     4561 ggcaacgtcc tgaagcatcg agctgtggtt gcagccgttg tgcgtgtgtt cgtctgtcat
     4621 gatcgacctc gcacgttacc atctgcacgt atgacgccga aagtcacccg tgccggcgat
     4681 cgaggccttc tcgccgattt cggacctgat gtcgctgccc tcgagcttca tgcgcgcgcg
     4741 gcagcgttgc gcgctcgcga cgacgtcatc gcatgtatcg tcggccatca gtcgctctac
     4801 gtgatctttc gcggagtgcc ggagctcgac ttcgacgatg tcccgccgat cacggtcacg
     4861 cctcgggtac acgtcatcga cgtcgacttc tcaggtcccg atctcgacga gcttctcgcg
     4921 cacacgcgcg ttacgcgcga cgcgttcctt gcccgcgttc cgtcgctgcg cctcaccgca
     4981 cgctacctcg gctttcgcgc cggcttcgcg tatctcgaag ggtggccgga ggagtggcga
     5041 atgccgcgcc gtccgcggtc gcggaacctc gtgccccgag gaagctttgc gatcgctggg
     5101 gcgatggcgg ggttctatcc cgtcgactcg ccggggggct ggaacatcct cgggcgcaca
     5161 aacgccacgc tctgggatcc aaatgtcgag ccgcccaatc ggttcgctcc cggcgacgtc
     5221 gtccagcttc gcgcggttcc gatcgatcgc ttcgatgcgc cacctggcga tcgcgtcgtc
     5281 gcgagtggag aggttgttgc cgaggtcgtg atgccgggcc agctcacgac gattgttggc
     5341 gcccgcgact ggaagcgcgc cgcgtacggt gtctcgccgg gcggagcgtt cgacgcgatg
     5401 gcggcggcga gcgcgaatcg cgcggctgga aacggcgacg tgccgttgct ggagtgcgtg
     5461 ctggtcgcgc cgcgattgcg gtttcggacg gcgagagttg cggcgttctg cgatggaagc
     5521 ggtttggtac gaacgttcac gttgtctgcc ggagaggcgc tcgacatcgg gcgttttcat
     5581 ggcggtcttc atggatacct ggccatcgag ggcggtatcg acgagatgcg ggcatcgttt
     5641 gcggaggggc cgcatgtgct gcggaagggg gatgagcttc gggtggccgg tggtgggtcg
     5701 tctgccgttg cgcagtcatt cgacgggcgg cgcgatcctc atttgattcg agtcattgca
     5761 gggccgcacg aggcgccgcc gctgccggag gagtgggagg tgacttcgga gatgaatcgg
     5821 attgggattc gacttcgccg ccgggccggc ggcggaacgg ccggcgggcc gccggcgcta
     5881 caccaaacgg atgcgcgaca cgaccttcgc gagctgcctt cgtgcgggat gcagtttggg
     5941 acgctacagt ggcatccgga tgggagtctc gtagccatgg ggccggatca tccggtcacg
     6001 ggcggctatc tccagcctgc gaccgtgatc tcggaggatt tgtggaagct cgcccagctt
     6061 gcaccgggcg aaaggatcag gttcgaagtt gtcggtgaag cgtgacgcag agacagttct
     6121 gcggttcgcc gctcagtgag gcggaaaaat ccgagtctcc cactccccac ccccgacacc
     6181 ccactcccta catcgacgac atcgcgttca ggaactcgga gtttgttctt gtcttttcga
     6241 gtttctcgac gagcagctcc atcgactcga ccgtcgacag cggcgagaag actttgcgca
     6301 gaacccagat gcgattgagc tcgttgcgct cgaggagcag ttcctctttg cgcgtgcccg
     6361 acttgttgat gtcgatggca gggaagacac gcttgtcgga gagcttgcgg tcgaggtgca
     6421 cttccatgtt gccggtgcct ttgaactctt cgaagatgac gtcgtccatg cgcgagccgg
     6481 tgtcgatcag cgcggtggcg atgatcgtca gcgagccgcc ttcttcgacg ttacgcgcgg
     6541 agccgaagaa gcgcttcggg cgctgcaggg cgttggaatc gacgccgccg gagagaacct
     6601 tccccgacgg cggcacgacg gtgttgtagg cacgcgccag acgggtaatc gagtcgagaa
     6661 gaatgacgac gtcgcggcga tgctcgacca ggcgcttggc cttctcgatg accatctcgg
     6721 cgacctgcac gtggcgcgaa gccggctcgt cgaacgtcga ggagatcact tcgcccttca
     6781 cgctgcgctg catgtcggtg acttcttcag gccgctcatc gatgagcaga acgatcagcg
     6841 tcacttcagg atggttgctg gtgatggagt tggcgatagc ttgcagaatc atcgtcttac
     6901 cggtacgcgg cggagcgacg atcagaccac gctgtccttt gccgaccggc gcgatgaggt
     6961 cgagcacgcg cgacgacatg ttcgacggca cttccagctt gatgcgctgc tcgggataga
     7021 gcggcgtcag gttgtcgaag aggatcttct cgcgcgcgac atcggggtgc tcgaagttga
     7081 ccgcctcgac tttgatgagg gcgaagtagc gctccccttc cttcggcggg cgaacctggc
     7141 cggaaacggt gtcgccggtg tgaagatcga agcggcggat ctgcgacggc gaaacgtaga
     7201 tgtcgtccgg accgggaaga tagttgtact cgggcgcgcg cagaaagccg aaaccgtccg
     7261 gcaggcactc caggactccc tcggaaaaga tgagtccgga cttttcggtc tgcacttgca
     7321 ggactgagaa gatcagatcc tgtttgcgca tccccgtggc gttctcgatg ccgtgctcct
     7381 tggcgacttt cgtcagttcc gggagcttca tctctttgag cacgcggata tcgagcgtct
     7441 cggcggccag cgtcgggtcg ctgcgcttgt tgaggatcgc cggagcgggg cggtcgttct
     7501 cgacatcgac cggcggaggc gacgcgacga gcggctcgtc ttcggcatca gcctcgttgc
     7561 cgtttccact cacctcgact gccggctcga ccgctggatc gtcagcggaa actggatcct
     7621 cgtcaggagc ctcttcagcc acctcggccg gtgcggcaac cttctccgct ttcggtttgc
     7681 gcgtggtttt acgcacgggc ttttccgctt tcgggacggg cagctcggct acagctaccg
     7741 gagtttcatc ttcgaattca gtttcagaca ttgttcagac ctcattgatt gggatggcat
     7801 tgctagagcc gcgaagcgat ctcgcgccgt acttgctcga ttcctgtctt cgtgatgacc
     7861 gaactggtga tgagccggtc ttcggtgatc tcgagctctt tcgcgatcgc tgcgcggctc
     7921 ttgccgagtt ggttgttcga gagtttgtcg gttttggtca ggaggattcg ttcgtcgatt
     7981 cccgaagcga ccgtccagtc gtgcatttga cgatcctcgg ggctgggagt tcgccgcaca
     8041 tcgagaagaa tgaaaatcag tttgagcgca tcggcgctct ccaggtatcc gcgcacgagc
     8101 tcgccccagg agcgggcgat gtcgcgcgga acttttgcgt atccgtaacc cggaaagtcg
     8161 acgatgtaga agcgttcatt gatgagatag aactgaatgg cctgcgtttt tccgggcgtc
     8221 tggctcacgc gggcgattcc ttttcgtttc agaaccgcgt tgatgacact cgattttccg
     8281 acgttcgacc ggccgaccat cgcgaattgc ggtctgcggt cagtcggaaa gtcgtcggct
     8341 ttgtacgccg cccgggagaa ctcgactgtt ctgatgtcca agagattggt tgttaaggga
     8401 aatgcaaagc agaggtgctg cgaagaggta ctagctgggt tttggatgat taacgacgga
     8461 gattgtgcag gtattctcgc ctttcggtca agagatggtc acggaggggt gaggatggaa
     8521 ggtccgtgcc agtggagggg ggtggggagt tgggggtggg gtgtgggagg gactcgcgtc
     8581 gagacagatc ccatgtggaa gtgacgcgat ggttgccgag cgccgctctc acaggcgctc
     8641 agcgccatcg agcttggcca ggttcttgtc gaacgtgccg agcgggagat ggccggcttt
     8701 gcgcgcgatc tcaatcacta ggcaatcgga gaacccaagc gagggtctga gtcgatactg
     8761 atccagcgcc gaggtcacga catccgcgtc ctgaagactt accgcttcct gattgagtag
     8821 catctcgatc gctgcggcga tacctgcatc atcgagtttg tatgccctcc gcagtaccca
     8881 cgcggtctcg acaagaacga catgggaaat ccatacgccg cgattgacga aggcctctgc
     8941 caccaccgcc tgtctttcat cgtcgccggt gatcaggcga acgagaacat tcgtatcaac
     9001 ggcgcgcatg cttctctaga aggtgatcgc caatcgcttg cttgagctct tccagtgaac
     9061 gagccctcgg gcgcgtcgga aacagggcgc gtcgaatatc ttcgaacgag tacttaccag
     9121 ctttgcgaac gactatgtca tcgccatggt gatgccattc gagaaccgag cctgggccta
     9181 tgccgagctc ttttctgatc tcggcaggaa cggagatctg tccctgagca gtgaccttcg
     9241 attgcggtag agccatgcct ccatagtacc acggtactat ggaggagtgt cgactggcgg
     9301 cgaggcgccg ccagtccggt aacaggctca gtgaatcggc ggttccgata cgacggcgtc
     9361 gggcagcttc ttcacgagcg gcacgatcgt gccgtcgagc gcgtgcagga cgacctcgtc
     9421 catcgactcg acgaggttga actccatctt ttcgcggaca tccgcgggga tgtcttcgag
     9481 atcgcgctcg ttctcggcgg gaagaataat gcgcgtgatg ccggcgcggg cagcggcgag
     9541 gatcttgtcc ttcaccccgc caaccgggag caccttgccg cgcagggtga tctcgccggt
     9601 catggcgatg tcgcgatgca cggggatgcg ggtgacggct gacaggagcg ccgtcgccat
     9661 cgtgatgccc gccgatgggc catccttcgg gatcgcaccc tcgggaacgt ggatgtggat
     9721 gtcgaggctg gcgtggaaat cggcatcgag accgaacaac tccgcgcgcg aacgaacgta
     9781 ggacagggcg gctcttgccg attcctgcat cacgtcgccg agcttgccgg tgagcgtaag
     9841 gttgcctttg ccgcgcgaga tggctacctc ggtcgacagg atctcaccgc cgacttcggt
     9901 ccaggccaga ccggtagcca gaccgatctc gctcttgtcg tggatcgttt gcgagcggaa
     9961 ctttggcttg ccgaggagct cgcggacctt ggagcggtcc atgatggtct tgatctcggg
    10021 accttttttc acgacctgac gggcgatctt gcgcaggacc gaaccgatct cgcgttccag
    10081 gctgcgaaca ccggcctcgc gcgtgtacga gtcgaccagc tcttcgatgg cagtgtcgag
    10141 aaaatcgacc tggctctcct cgatcccctg ctcctggcgc tgcttcggaa cgaggaactg
    10201 gcggccgatg gcgagcttct cgaggtgcgt gtagccggag agcgagatga tctccatccg
    10261 gtctttgagc ggagccggaa tggtgtgcgt cacgttggcc gtggcgatga acatcacctt
    10321 cgacaggtcg tactcgacgt cgagatagtg atcggtgaag gcgttgttct gctcgggatc
    10381 gaggacttcg agaagggcgg ccgagggatc gccgcggaag tccatcgaca tcttgtcgac
    10441 ttcatcgagg aggaagaccg ggttgatcgt gcctgccttt ttcatccgct ggatgatctg
    10501 gccggggaac gcgccgatat aggtccgccg atgcccgcga atctccgcct cgtcgcgcac
    10561 gccgccgagg gaaaggcgaa cgaacttgcg gccggtggcg cgggcgatcg atttcgccag
    10621 cgacgtcttg ccgacgcccg gcggtccgac gaagcagagg atcgtcccct tcggtccttt
    10681 gatgagctga cgcacggcca ggaactcaag gatgcgctct ttgatcttgt cgaggccgta
    10741 gtgatcttcg ttgaggatcg actcggcgag atcgatgtcc ttgatctc
//
LOCUS       DATLVW010000963         4022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_412740, whole genome shotgun sequence.
ACCESSION   DATLVW010000963 DATLVW010000000
VERSION     DATLVW010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412740"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VNN25_28525"
     CDS             <1..554
                     /locus_tag="VNN25_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXH95556.1"
                     /translation="RIDREALEMEVSLDHDPPAALPLTLILALPRPKVLNRVIASAAS
                     LGIKRIVLINAWRVEKSYWKSPRLSPENLRNQSILGLEQSRDTVIPSIYTRRFFRAFV
                     EDELPSLARGTRALVAHPHALESCPRDVREPVTLVIGPEGGFIDAEIASLQAAGCTPV
                     TIGERILRVETVVPYLAARLLPP"
     gene            complement(614..1369)
                     /locus_tag="VNN25_28530"
     CDS             complement(614..1369)
                     /locus_tag="VNN25_28530"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXH95557.1"
                     /translation="MNEDARPIALLRLSKDRLEELFPIELSDADPLSTAEPSIGGLVQ
                     LNSGPYAVVIYGKITGETEVSLPVSAPMARHWAAFLCEVPLGAKEIVWTSDRIANARS
                     QGSLTQIGNIIERRSLAHASPSDTVRAVAKKMSDRNIGAIAVLDSGRLVGIFSERDVM
                     RRIVAEGRNPDETRVDAVMTKDLIVAAPGDDIDDALLRMHSCNCRHLPVVDRGHFLGM
                     VSMGDLLQIDVDNSRARAILLLELVTESRDYEN"
     gene            1523..1669
                     /locus_tag="VNN25_28535"
     CDS             1523..1669
                     /locus_tag="VNN25_28535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95558.1"
                     /translation="MKNSIPHRPGGNDPKRAFTLGNSPAAMFLSGRPPINPVLLISSF
                     AAAS"
     gene            complement(1708..>4022)
                     /locus_tag="VNN25_28540"
     CDS             complement(1708..>4022)
                     /locus_tag="VNN25_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HXH95559.1"
                     /translation="KEDADSLAIRITAHNRGPERAPLHFIPQLWFRNTWSWSGEQVAA
                     PSITAASGASGNPAIRADDTHTPALAGLLHDAHVGPYTLEFPAGTQLLFTDNETNGPR
                     VWGPGAQSRSRFVKDAFHRHIVNGEHGAVNPEQIGSKACGWMRAEIEPGASHTMSMRL
                     RPTGAKVDAASLEQSVEATIDLRRAEADEFYNSIHPARATAEERNIQRQAFAGLLWSK
                     QSYLLDVDIWLDGDDPSDPPPAERQHGRNARWRHLNSMRILSMPDKWEYPWFAAWDLA
                     FHTVVHALIDAHFAKEQLWLMLFEQFQHPNGQLPAYEWEFSDLNPPVHAWAVWRVYNM
                     DRIRNGGDRPFLEKCFHKLMLNFTWWVNKVDREGNNVFEGGFLGLDNIAVFDRSETLP
                     GGASLEQSDATGWMGMFSLVMMRMALELAKDNPTYEGLATKFFEHYVYIGAAMKMMRG
                     NRTLWDDADGFFYDLLRFPDGRNVPFRVRSLVGLIPLYAVERLERKWVEPFAVFRENF
                     DWFMKHRADIVEHSVTTAHHDGETTHVLAVVDQNQLQHLLARVADPDEFLSTYGLRSL
                     SKAHQAQPFVFGNASVTYEPAEASTKLKGGNSNWRGPIWFPTTFLMIETLRKLEKAYS
                     GNVRIAIGNGHDPSHETPPHNDPDTTLADTRADEALGAGAARPRRRGMTLTNVPVPPP
                     ENTTTLTGLAADLAKRMTSIFLRDADGYRPAHGPRGKRQAELFAHDPHWKDLIWFFEY
                     FNGDTGEGLGASHQTGWTALVASLIDEWSV"
BASE COUNT          742 a         1222 c         1248 g          810 t
ORIGIN      
        1 cgcgcatcga ccgcgaagcg ctggagatgg aggtatcgct cgatcacgat ccgccggctg
       61 cgttgccgct caccctgatc cttgctctcc cgcgaccaaa ggttctcaac cgtgtcatcg
      121 ccagcgcggc gtcgctggga atcaagcgaa tcgtgctgat caacgcctgg cgcgtggaga
      181 agagctactg gaaatcccca cgcctttctc ccgagaacct gcggaatcaa tccatcctcg
      241 gcctggaaca atcgcgcgac acggtgatcc cgtcgatcta tacgcgccgt ttctttcgag
      301 cgttcgtcga ggacgagctg ccttcgctgg cacgcggaac gcgcgcgctg gtcgcgcatc
      361 cgcacgcgct agagtcctgc ccacgcgacg ttcgcgagcc tgtcacactg gtcatcggcc
      421 ccgagggcgg cttcatcgac gcggagatcg catcgctgca agcggccgga tgcactccgg
      481 tgacgatcgg agagaggatt ctgcgcgtgg agacggtggt gccgtatctg gcggcgcggt
      541 tgttgccgcc gtaaacttcg tcgccgtgtc gggttgtcat tcgctgccgt tcgacaactt
      601 ggttataggt aggtcaattc tcatagtccc gcgattcggt caccaactcc agcaagagaa
      661 tggcgcgagc gcggctatta tcgacgtcga tctgtaggag gtcgcccatc gagaccattc
      721 cgaggaagtg accccgatcg acaacgggaa ggtgacggca gttgcaagag tgcatcctga
      781 gcagcgcgtc gtcgatatcg tcccctggcg cggcaacgat gaggtctttt gtcatgaccg
      841 cgtcgacgcg cgtctcatcc gggttacgtc cctcggcgac gatgcgcctc attacgtcgc
      901 gctccgagaa gatacccacg agcctgccgg aatcgagcac cgcgatcgcg ccaatgttgc
      961 gatcgctcat ctttttggcc actgcacgaa cagtatctga aggcgacgcg tgagctagcg
     1021 aacgccgctc aatgatattt ccgatctgcg tcaaggaacc ttgtgatcgc gcattcgcga
     1081 tcctatcgga cgtccagaca atctccttcg cgcccaaggg gacttcgcag aggaaagctg
     1141 cccaatggcg agccattggc gcactcacgg gcaaggacac ctcggtttca ccagtaatct
     1201 tgccgtagat gacaaccgca taagggccgc tgttgagttg cacgagccca ccaatcgatg
     1261 gctcggcggt cgaaagggga tcagcatcgg acaattcgat cggaaacaat tcctcaagac
     1321 ggtctttcga gagccggaga agcgcgatcg gtctcgcatc ttcgttcaac tcctcgggac
     1381 ttgctcttct cactggtcaa ctcgctttac aattctagca gcgattagca caacatccgc
     1441 gtcatcgtcc gatcgcacta cgcctgcttt tccgatggtc catgtaggtg attgaggtac
     1501 atcatcatcc ggagcaatgg tcgtgaagaa ttcgatcccg catcgaccgg gtggcaacga
     1561 tcccaaacgc gccttcaccc tcggaaacag tccagccgca atgtttctgt ccggccgccc
     1621 tccgatcaac cctgtgctgc tcatcagctc gtttgcagct gcatcctgaa accggtggaa
     1681 cggcccatat tcacctggcg ccgccaatca cacgctccac tcatcgatca gactcgccac
     1741 cagcgcggtc catcctgtct ggtgtgaggc gccgaggcct tcgccggtgt cgccgttgaa
     1801 gtattcgaaa aaccagatca ggtctttcca gtgggggtcg tgcgcgaaga gctcggcttg
     1861 tcgcttgcct cggggaccgt gggcggggcg gtatccgtcg gcgtcgcgga ggaagatgct
     1921 ggtcattcgt ttggccagat cggcggccag gccggtgagc gtcgtcgtgt tctcgggcgg
     1981 cggaacagga acattcgtca gcgtcatgcc gcggcggcgt gggcgggcag ctccggcgcc
     2041 gagggcctcg tccgcgcgcg tgtcggcgag agtcgtgtcg ggatcgttgt gcggcggtgt
     2101 ttcatgcgag ggatcgtgtc cattgccaat cgcgatgcgc acgttgccgc tgtacgcctt
     2161 ctccagttta cgcagcgtct cgatcatcag gaacgtggtc gggaaccata tggggccgcg
     2221 ccagttgctg ttgccgcctt tgagtttcgt cgatgcttcc gccggctcgt aggtcaccga
     2281 cgcgttgccg aagacgaacg gctgcgcttg atgtgccttg ctaagactgc gcagtccgta
     2341 cgtcgagaga aactcgtccg gatcggcgac acgcgcaaga agatgctgga gctgattctg
     2401 atcgacgacg gccaaaacat gcgtcgtctc gccgtcgtga tgcgccgtcg tgacggagtg
     2461 ctcgacgatg tcggcgcgat gtttcatgaa ccagtcgaag ttctcacgga agacggcgaa
     2521 cggctcgacc cacttccgct ccagccgctc gacagcgtaa agcggaatca gtcccacgag
     2581 cgagcgaacg cggaacggga cattcctgcc gtcagggaat cggagcaggt cgtagaagaa
     2641 gccgtcggcg tcatcccaca acgtgcggtt gccgcgcatc atcttcatcg ccgcaccgat
     2701 gtagacgtag tgctcgaaga atttggtggc caacccttcg taggtcggat tgtctttggc
     2761 caactccaag gccatgcgca tcatcacgag cgagaacatc cccatccagc cggtggcgtc
     2821 gctctgctcc agtgacgcgc cgccgggaag tgtttcgctg cggtcgaaca cggcgatgtt
     2881 gtcgagcccg aggaagccgc cttcgaagac gttgttgcct tcgcggtcga ccttgttcac
     2941 ccaccaggtg aagttcagca tcaacttgtg aaagcacttc tcgagaaatg gccggtcgcc
     3001 gccgttgcgg atgcggtcca tgttgtagac gcgccagacg gcccaggcgt gcaccggagg
     3061 attgaggtcg gagaactccc actcatacgc ggggagctgc ccgttcggat gctggaactg
     3121 ttcgaacagc atcagccata gctgttcttt ggcgaagtgc gcatcgatga gcgcgtggac
     3181 gacagtgtgg aaggcgaggt cccaggcggc gaaccacggg tactcccatt tgtcgggcat
     3241 cgagaggatg cgcatcgagt tgagatggcg ccaacgcgca ttgcgtccgt gctgtcgttc
     3301 ggccggaggc gggtcgctgg ggtcgtcgcc gtcgagccag atgtcgacgt cgagcaggta
     3361 gctctgcttg gaccagagca gtcccgcgaa ggcctgcctc tgaatgttcc tctcctcggc
     3421 agtcgcacgc gccggatgaa tgctgttata gaactcgtcg gcctcggcgc gccgaagatc
     3481 gatggtggcc tcaacgctct gctccagcga ggcggcgtca accttcgccc cggtcggccg
     3541 caaacgcatc gacatcgtgt gggatgcacc cggctcgatc tcggcgcgca tccatccgca
     3601 cgctttcgat ccgatctgct ccggattcac ggcgccgtgc tcgccattga cgatgtggcg
     3661 gtgaaaagca tctttcacga agcggcttcg cgattgcgcg cccggtcccc atacccgagg
     3721 accattggtc tcgttgtcgg tgaagaggag ctgcgttccg gcgggaaact cgagtgtgta
     3781 cgggccgacg tgcgcgtcgt ggaggaggcc ggccagagcg ggcgtatgcg tgtcgtcggc
     3841 ccgaatcgca ggattgccac ttgctccgct tgcagcagta atggacggag ccgcaacttg
     3901 ctccccgctc cagctccatg tattccggaa ccagagctga gggatgaaat gaagcggcgc
     3961 gcgttcggga ccgcggttgt gcgcagtgat gcgaatcgcg agcgagtcgg cgtcttcctt
     4021 gg
//
LOCUS       DATLVW010000964         4432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U13202
            k141_413169, whole genome shotgun sequence.
ACCESSION   DATLVW010000964 DATLVW010000000
VERSION     DATLVW010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942616
            Sequence Read Archive: SRR7687010
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 14:32:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,712
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 50
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 4 of 29
            Pseudo Genes (incomplete)         :: 25 of 29
            Pseudo Genes (internal stop)      :: 2 of 29
            Pseudo Genes (multiple problems)  :: 2 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413169"
                     /isolate="SMAG_U13202"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.4449 N 87.0297 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VNN25_28545"
     CDS             complement(<1..468)
                     /locus_tag="VNN25_28545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95560.1"
                     /translation="MNRTSRFFRQVWRVNALLILVAAAAAAFAVCAFVWSQLEGSLRR
                     QSAETVAPIIPGKPATHELHLGGLVAIDGTSLYRATLSTPADRSGSSFSSGGYSSDTR
                     NILVVDGASATSHWLLPTDKELIVFNQDVGNPESKEGHAQPPLATVVLVKTLSP"
     gene            complement(525..722)
                     /locus_tag="VNN25_28550"
     CDS             complement(525..722)
                     /locus_tag="VNN25_28550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXH95561.1"
                     /translation="MAPNADSNCRLCGSRVPESELRRQLCPVCDEYAQHVALQSNKGL
                     DRIVGELQNGAVFRAALRNGH"
     gene            801..2153
                     /gene="der"
                     /locus_tag="VNN25_28555"
     CDS             801..2153
                     /gene="der"
                     /locus_tag="VNN25_28555"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXH95562.1"
                     /translation="MATSLKKIVIVGRPNVGKSTLFNRLAGKRRALTHDLPGMTRDRL
                     SDVITLDNGRRFELTDTGGLEYGDSPMSAFAGEIRDQARRALDTADFILFVVDGSAGI
                     LTEDRDIAQELRAASDRTLLLVNKVDRKDSAENVSDFYELGYNRILALSAEHGEGVDE
                     LFDIIGEIVPEDSIDETEEGDLGPIRIAIIGRPNVGKSSLLNRLANEERAVVSPISGT
                     TRDAIDSEIERNGQRYLIIDTAGIRRKGKTHDEAEKLSVISARKAIERCEIALVMIDA
                     VDGVTGQDATVAGYAEDAGRAAMLLVNKWDAAAHTQDDYKKVEEEIRFKLKFLPYAPI
                     EFISAKTGRRVEKVFPRIDEIMTGYRKRFTTSQLNQILERAIATHQPPALRGRPRRFY
                     YTTQLKAAPPTFALFSNMDDPLHFSYRRFLENQFREALGLVGCPIHLVLRARKGMKKE
                     "
     gene            complement(2241..4376)
                     /gene="glgX"
                     /locus_tag="VNN25_28560"
     CDS             complement(2241..4376)
                     /gene="glgX"
                     /locus_tag="VNN25_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXH95563.1"
                     /translation="MLRGRRSESTVLVWPGHPYPLGATYDGAGTNFSLFSEVATRVEL
                     CFFDNAGNQRCVDLPEMTGFIWHGYVPSVSPGQRYGFRVHGPWDPGAGQRCNPSKLLL
                     DPYGKAVDGAVRWNEAVFGRYFDNPDVRNDADSAPFMPHSVVTNPLFNWAGDRYPRTP
                     WHETIIYELHVKGFTQRHPDIPEQLRGTYAGLAHPAAISHLKTLGVSAVELLPVHQFI
                     HDSLLIERGLRNYWGYNSISYLAAHDEYSSGGAAGQQVHEFKQMVKTLHTEGIEVILD
                     VVYNHTAEGNHLGPMLSMKGIDNAAYYRLMPDDPRYYMDYTGTGNSLNMRHPHVLQLI
                     MDSLRYWHLEMHVDGFRFDLASTLARELHDVDRLSAFFDIIQQDPVLSQVKLIAEPWD
                     VGEGGYQIGNFPPLWSEWNGRYRDSVRDYWRGTDQVLGEFANRLTGSSDLYAWTGRRP
                     YASINFITAHDGFTLRDLVSYNEKHNDANGENGKDGADDNRSWNCGAEGPTDDEAINE
                     LRSRQQRNFLTTLILSVGVPMICGGDELGRTQSGNNNAYCQDNEISWLDWEHVDDGLF
                     AFVSRVIAFRRQHPVFQRRRWFYGRPLRGANVSDIGWFRPDGGEMTDDDWQSATARSI
                     GVFFSGEGIPAPDERGQPIVDDSFLILFNAHYEPMPFRLPMGPWGDRWESAIDTSHPV
                     PDLRAHHELLAGEEVVVEAYAMLVLRRVR"
BASE COUNT          868 a         1346 c         1407 g          811 t
ORIGIN      
        1 cggggagagt gttttgacta atacaaccgt tgcgagaggc ggctgcgcat gtccctcctt
       61 gctctcaggg ttgcctacat cctggttgaa gacgatcagc tctttgtcgg ttgggagaag
      121 ccaatgggaa gtggctgatg cgccgtccac cacgagaatg ttccgcgtat ccgaggaata
      181 gccgcccgag ctgaagctgg agccactccg gtcagcaggc gtcgaaagag tcgcgcgata
      241 gagggaagtg ccatcgattg caacgagacc accgagatgg agctcatggg ttgccggctt
      301 cccgggaatg atgggtgcca ctgtctcggc tgactggcgg cggagactgc cttcgagttg
      361 cgaccacacg aaggcgcaga cagcaaaggc agccgcagcc gctgcaacga gaatcaggag
      421 cgcgttgact cgccacacct gtctgaagaa tcgcgaggtt ctgttcatcc tcggagtgta
      481 actgacagga atcgtttact gcgcggccct tgcgggccac gcaattaatg tccgttgcgc
      541 aaggccgccc tgaacaccgc tccgttctgg agttcgccga cgatgcggtc gaggcctttg
      601 ttgctctgca gggcgacgtg ctgcgcgtac tcatcgcaga cggggcagag ctggcggcgt
      661 agctcgcttt ccggaacgcg cgatccacaa agcctgcagt tggaatcagc gttcggagcc
      721 atgtgtgaca ttgtagcatt cgccgtgccg ccgccctctc ggatttcgcg cgcggcgaag
      781 gaaatcccag aaggaaacga atggctacca gcctcaagaa aatcgtcatc gtcggacgtc
      841 cgaatgtcgg aaagtcgacg ctcttcaacc ggctggccgg gaagcgccgc gccctcacgc
      901 acgacctgcc ggggatgaca cgcgaccggc tctccgacgt catcacgctc gacaacggac
      961 gacggttcga gctgaccgac acgggcggac tcgagtacgg cgactcgccg atgtccgcct
     1021 tcgctggcga gatccgcgat caggcccgcc gcgccctgga cacagccgac ttcatccttt
     1081 tcgtcgtcga tggctccgca ggcatcctca cggaagaccg cgacatcgct caggagctgc
     1141 gcgctgcctc ggatcgcacg ctcctgctcg tcaacaaagt cgaccgtaaa gactcggcgg
     1201 agaacgtctc cgacttctac gagctcggct acaaccggat tctggcgttg tccgccgagc
     1261 atggtgaagg agttgacgag ctgttcgaca tcatcggcga gatcgtgccc gaggattcga
     1321 tcgatgagac ggaggaaggg gatcttggac cgatccgcat cgccatcatc gggaggccga
     1381 atgtcggcaa gtcatcgctg ctcaaccgcc ttgcgaacga agagcgcgcg gtggtctcgc
     1441 cgatctccgg gaccacgcgc gatgccatcg actccgagat cgagcgcaac ggccagcgtt
     1501 atctgatcat cgacaccgcc ggcattcgcc gaaaggggaa gacgcacgat gaggcggaga
     1561 agctctcggt catctccgcg cggaaggcaa tcgagcgctg cgagatcgcg ctcgtcatga
     1621 tcgatgcggt cgacggcgtg accggacagg atgcgaccgt tgccggatac gccgaggatg
     1681 cgggcagggc tgcgatgctg ctggtgaaca agtgggatgc cgccgcgcac acgcaggacg
     1741 attacaagaa agtggaagag gagatccgct tcaagctgaa gttcctgccg tacgctccga
     1801 tcgagttcat ctcggcgaag accgggcgcc gcgtcgagaa agtctttccg cgcatcgacg
     1861 agatcatgac cggctaccgc aaacgcttca cgacgtcgca actgaaccag atcctggagc
     1921 gcgccatcgc cacacaccag ccgcccgccc tgcgcggccg cccgcgccgg ttctactaca
     1981 ccacgcaact caaggccgca cccccaacgt tcgccctctt ctcgaacatg gacgacccgc
     2041 tccacttctc ctaccgccgc ttcctcgaga accagttccg cgaagccctg ggcctcgtcg
     2101 gctgcccgat ccatctcgtg ctaagagcgc gcaaagggat gaagaaggag tagtactact
     2161 cacgcgaaga cgcgaaggcg cgaaggttca aggattggat ctctccttcg tgtcttcgcg
     2221 tcttcgcgtg agaggtcttt ttaccgcacc cgccgcagca ccaacatcgc gtaggcctct
     2281 acgacgacct cttcgccggc gagcagctcg tgatgcgcgc gcagatcggg gacggggtgg
     2341 ctcgtgtcga ttgcagactc ccatcgatcg ccccatggac ccatcggcag gcggaacggc
     2401 atcggctcgt agtgcgcgtt gaagaggatg aggaagctgt cgtcgacgat cggctggccg
     2461 cgttcgtcgg gggcggggat gccctcgccg ctgaagaaga ctccgatgct gcgggcggtc
     2521 gcgctctgcc aatcgtcgtc ggtcatctcg ccgccgtcgg gcctaaacca gccgatatcg
     2581 ctgacgttgg cgccgcgcag tgggcggcca tagaaccagc gccggcgttg aaagacggga
     2641 tgctggcggc gaaaggcaat cacgcgcgag acgaacgcga atagtccgtc gtcgacgtgc
     2701 tcccaatcga gccacgagat ctcgttgtcc tggcagtaag cattgttgtt tccgctttgc
     2761 gtgcgcccga gctcgtcgcc gccgcagatc atcggcacgc ccaccgagag gatcaacgtc
     2821 gtcaggaagt tccgctgctg tcgcgaccgt aactcattga tcgcctcatc atccgtcggt
     2881 ccctcggcgc cgcaattcca cgaccgattg tcatccgcgc cgtctttgcc gttctcgcca
     2941 ttcgcgtcat tgtgcttctc gttgtacgag acgagatcgc ggagtgtgaa gccgtcatgt
     3001 gcggtgatga aattgatcga tgcgtacggc cgcctgccgg tccacgcgta gaggtccgag
     3061 ctgccggtca agcgattcgc gaactcgccg aggacctgat cggtgccgcg ccagtaatcg
     3121 cggacggaat cgcgataacg accgttccac tctgaccaga gcggcggaaa gttgccgatc
     3181 tggtagccgc cttcgccgac atcccacggc tcggcgatga gcttgacctg cgacagaacc
     3241 gggtcctgct ggatgatgtc gaagaacgcg ctgagccggt cgacgtcgtg cagctcgcgg
     3301 gcgagcgtcg atgccagatc gaaacggaat ccatcgacgt gcatctccag atgccagtaa
     3361 cgcagcgagt ccatgatgag ctgcagcacg tgcggatgcc gcatgttcag gctgttgccc
     3421 gtgccggtgt agtccatgta gtagcgggga tcgtcgggca tgaggcggta gtaggcggcg
     3481 ttgtcgattc ccttcatcga caacatcggc ccgaggtgat tgccctcggc ggtgtggttg
     3541 tagacgacgt cgaggatcac ctcgatccct tccgtgtgaa gcgtcttcac catctgcttg
     3601 aattcatgaa cctgctggcc ggccgcgccg cccgatgaat actcgtcatg agccgcgaga
     3661 tagctgatcg agttgtaacc ccagtagttg cgaaggccgc gctcgatcag caatgagtcg
     3721 tgaatgaact gatgcacggg gaggagctcg acggcgctga cgccgagggt cttgagatga
     3781 ctgatggccg cgggatgggc gaggccggcg taggtaccgc gcagctgttc gggaatgtcc
     3841 ggatgccgct gggtgaagcc tttgacgtgg agctcataga tgatcgtctc gtgccacggc
     3901 gtgcggggat agcgatcgcc agcccagtta aagagcggat tcgtgacgac cgagtgcggc
     3961 atgaacggcg ccgagtcggc atcgttcctg acatcgggat tgtcgaagta gcgtccgaag
     4021 accgcttcgt tccaccgaac cgcgccatcg accgctttgc cgtaaggatc gagcagtagc
     4081 ttcgatggat tgcaacgctg tcccgcgcca ggatcccacg gtccatgcac acggaatccg
     4141 tagcgctggc caggactgac gcttggcacg tagccatgcc agatgaagcc ggtcatctcc
     4201 ggcaggtcga cgcagcgctg attgcccgca ttatcaaaga agcagagctc gacgcgagtc
     4261 gccacttcgg aaaagagcga gaaattggtt cccgcgccgt cataggtagc acccagcgga
     4321 taagggtggc ccggccacac cagtaccgtt gattcgctgc ggcgtccgcg gagcatgttc
     4381 acaggtgagc tcggcaaggt gtgtgccatg ggcggagggt ggggagtggg ag
//
